bluuebunny/crossref_metadata_2025_split huggingface dataset:
DArTseq genotyping facilitates identification of Aegilops biuncialis chromatin introgressed into bread wheat Mv9kr1
Authors: Gaál Eszter , Farkas András , Türkösi Edina , Kruppa Klaudia , Szakács Éva , Szőke-Pázsi Kitti , Kovács Péter , Kalapos Balázs , Darkó Éva , Said Mahmoud , Lampar Adam , Ivanizs Laszlo , Valárik Miroslav , Doležel Jaroslav , Molnár István 
Published: August 2024

Abstract

Aegilops species, wild relatives of wheat, offer valuable traits for wheat improvement through interspecific hybridization. Despite many Ae. biuncialis accessions maintained in gene banks, only a few have been utilized in wheat breeding. Gene transfer from Aegilops has been hampered by difficulties in detecting introgressed Ub- and Mb-genome chromatin at high resolution in the wheat background.
The present study applied DArTseq technology, to genotype two backcrossed populations (BC382, BC642) derived from crosses of a wheat line Mv9kr1 with Ae. biuncialis accession, MvGB382 (earlyflowering and drought tolerant) and MvGB642 (leaf rust-resistant).
11,952 Aegilops specific Silico-DArT markers were obtained, while 8998 markers were specific to wheat. Using the results obtained on diploid progenitors and wheat-Aegilops addition lines, 7686 markers were assigned to the Ub-genome chromosomes and 4266 for Mb-genome chromosomes and ordered by the use of chromosome assemblies of hexaploid wheat and Ae. umbellulata.
In the BC3 populations, 5.7% of BC382 and 22.7% of BC642 lines contained Ub-genome chromatin, while Mb-genome chromatin was detected in 88.5% of BC382 and 84% of BC642 lines. Notably, chromosomes 4Mb and 5Mb were frequently present in both populations, whereas chromosomes 3Mb and 6Mb were rarer. A Mv9kr1-Ae. biuncialis MvGB382 4Mb and 5Mb disomic addition lines were identified together with a new 5DS.5DL-5MbL recombination. Moreover, previously developed Mv9kr1-MvGB642 introgression lines were identified more precisely by DArTseq. A possible effect of the 5MbL distal region on seed length has been also observed and discussed. The new cytogenetic stocks represent valuable genetic resources for wheat improvement.
Resected Gastric Volume / Number of Staplers Fired Ratio as a Tool in Predicting Complication and Midterm Results in Sleeve Gastrectomy
Authors: Kizilkaya Mehmet Celal , Basaran Ceren , Kalafat Utku Murat , Mutlu Arda Ulaş , Saracoglu Can , Aytac Erman 
Published: August 2024

Abstract

Background
Laparoscopic sleeve gastrectomy is a restrictive surgery that aims to decrease the patients’ gastric volume. This study hypothesized that increased resected gastric volume/number of staplers fired ratio is associated with better postoperative 12th -month outcomes.
Objectives
To analyze the effects of resected gastric volume/number of staplers fired ratio on postoperative results of patients with sleeve gastrectomy.
Setting:
University Hospital, Istanbul, Türkiye
Methods
We analyzed the data of 407 patients who underwent laparoscopic sleeve gastrectomy between 2018–2022. The primary outcome was the effect of resected gastric volume/number ratio on postoperative 12th -month excess weight loss.
Results
A total of 407 patients were included in the study. The higher resected gastric volume/number of staplers fired ratio was associated with higher excess weight loss in the postoperative 12th -month, and no effect was found in complications (p = 0.001 and p = 0.785, respectively).
Conclusion
This study revealed that resected gastric volume/number of staplers fired could be used to predict weight loss. Further studies should inspect the effect of resected gastric volume/number of staplers fired on complications.
Distinct muscle regenerative capacity of human induced pluripotent stem cell-derived mesenchymal stromal cells in Ullrich congenital muscular dystrophy model mice
Authors: Goto Megumi , Takenaka-Ninagawa Nana , Zhao Chengzhu , Bourgeois Clémence Kiho Yoshioka , Miki Mayuho , Motoike Souta , Inada Yoshiko , Zujur Denise , Theoputra William , Jin Yonghui , Toguchida Junya , Ikeya Makoto , Sakurai Hidetoshi 
Published: August 2024

Abstract

Background
Ullrich congenital muscular dystrophy (UCMD) is caused by a deficiency in type 6 collagen (COL6) due to mutations in COL6A1, COL6A2, or COL6A3. COL6 deficiency alters the extracellular matrix structure and biomechanical properties, leading to mitochondrial defects and impaired muscle regeneration. Therefore, mesenchymal stromal cells (MSCs) that secrete COL6 have attracted attention as potential therapeutic targets. Various tissue-derived MSCs exert therapeutic effects in UCMD mouse models. However, no reports have compared the effects of MSCs of different origins on UCMD pathology.
Methods
To evaluate which MSC population has the highest therapeutic efficacy for UCMD, in vivo (transplantation of MSCs to Col6a1-KO/NSG mice) and in vitro experiments (muscle stem cell [MuSCs] co-culture with MSCs) were conducted using adipose tissue-derived MSCs (Ad-MSCs), bone marrow-derived MSCs (BM-MSCs), and xeno-free-induced iPSC-derived MSCs (XF-iMSCs).
Results
In transplantation experiments on Col6a1-KO/NSG mice, the group transplanted with XF-iMSCs showed significantly enhanced muscle fiber regeneration compared to the other groups one week after transplantation. At 12 weeks after transplantation, only the XF-iMSCs transplantation group showed a significantly larger muscle fiber diameter than the other groups without inducing fibrosis, which was observed in the other transplantation groups. Similarly, in co-culture experiments, XF-iMSCs were found to more effectively promote the fusion and differentiation of MuSCs derived from Col6a1-KO/NSG mice than the other primary MSCs investigated in this study. Additionally, in vitro knockdown and rescue experiments suggested that the IGF2 secreted by XF-iMSCs promoted MuSC differentiation.
Conclusion
XF-iMSCs are promising candidates for promoting muscle regeneration while avoiding fibrosis, offering a safer and more effective therapeutic approach for UCMD than other potential therapies.
Evaluation of Ocrelizumab (Xacrel) on Walking Ability in Multiple Sclerosis Patients: A First Report from Iran
Authors: Mahyad Mahshid , Saeidi Morteza , Kohandel Kosar , Ebrahimian Maryam , Baghaei Mahdieh , Jahani Shima , Nahayati Mohammadali 
Published: August 2024

Abstract

Introduction:
Ocrelizumab (OCR) and Rituximab (RTX) are monoclonal antibodies targeting CD20 on B cells, a promising approach for relapsing-remitting Multiple Sclerosis (RRMS) and primary progressive MS (PPMS). They aim to modulate the immune system and reduce B cells, potentially leading to fewer relapses and delayed disease progression. Xacrel, The Iranian-made Ocrelizumab biosimilar, requires further investigation for its effectiveness in MS treatment.
Objective
we aim to assess the effectiveness of Xacrel (Iranian Ocrelizumab) for MS treatment by evaluating alteration in expanded disability status scale (EDSS) score and timed 25-foot walk (T25FW) test. This study also explores the potential benefits of switching patients drug from RTX to OCR.
Material and Methods
This prospective cohort study at Qaem Hospital (February 2022–May 2024) on 143 MS patients evaluates Xacrel in MS patients using EDSS and T25FW scores before treatment and at 6 and 12months post-treatment. Additionally, we assessed 29 MS patients whose drug transitioned from RTX to OCR to compare the effectiveness of these treatments. For this purpose, MS progression was assessed using the EDSS score and T25FW test at baseline, six months, and twelve months after switching their medication.
Results
In our study, the average age was 38.48 ± 8.73 years, and over 70% were women. 76.2% were between 30–50 years old, with a mean disease duration of 6 years. About 19.6% were treatment-naive, with dimethyl fumarate as the most common first-line drug. Over 12 months, significant declines in EDSS scores and improvements in T25FW tests were noted at 6 and 12 months compared to baseline (all P < 0.05), but not between 6 and 12 months. Significant factors were RRMS for 6-month EDSS score changes (P = 0.011) and treatment-naive patients for T25FW at 6 months (P = 0.018) and 12 months (P = 0.004). Switching from Rituximab to Ocrelizumab showed no significant changes in EDSS or T25FW scores, despite trends of decreases in EDSS and increases in T25FW times at 6 and 12 months. Subgroup analyses by gender, age, disease duration, type, and previous medication history showed no significant differences.
Conclusion
Xacrel (Iranian-produced Ocrelizumab) effectively prevented EDSS progression and improved walking ability in treatment-naive RRMS patients, while switching from Rituximab to Ocrelizumab did not significantly impact disability scores or walking abilities.
New insights to select antigens to leishmaniasis diagnoses
Authors: Santiani Manuel Hospinal , Guzmán Yessenia Cristhel Manrique , Minozzo João Carlos , Vandenberghe Luciana Porto de Souza , Boschero Raphael Aparecido , Costa Jean Vedova , Santos Germana Davila dos , Peace Violetta Dias , Soccol Carlos Ricardo , Soccol Vanete Thomaz 
Published: August 2024

Abstract

The leishmaniases represent a group of chronic diseases with epidemiological diversity and a broad clinical spectrum that affect 350 million people worldwide. Early diagnosis and appropriate treatment are the best tools for controlling the disease. Immunological methods have been widely used as a screening tool for diagnosis. In this work a platform for the selection, production, and validation of antigens for the diagnosis of leishmaniasis was created. 540 peptides were obtained and selected through SPOT-synthesis and immunodetection using serum of visceral leishmaniasis (VL), where 14 antigens were selected. The antigens were valued by immunoassay test (ELISA) and intradermo reaction in experimental model. The best antigen for serological diagnosis of LV was Pep.S.LV57.p.395 with 80% of sensitivity and 92% of specificity. The avidity test demonstrated an improved sensitivity, which was able to differentiate patients with leishmaniasis from those with Chagas disease. For IDR a mix of six peptides were encapsulated in a cationic liposome and valued to observe cellular immunological. The best results, when compared with Montenegro antigen, was the induration on 72 h. This platform showed excellent results to search antigens and can be used for screening and in the diagnosis of neglected or emergent diseases.
Association Between Disconnected Networks and Surgical Outcome in Temporal Lobe Epilepsy
Authors: Huang Xiaoting , Zhou Chunyao , Guo Danni , Du Yangsa , Xie Fangfang , Feng Li 
Published: August 2024

Abstract

The purpose of the current study was to assess the change characteristics of structural network connection strength (SNCS), functional network connection strength (FNCS), and structural-functional correlation (SFC) of preoperative disconnected networks in temporal lobe epilepsy (TLE) and the capability of these measures to predict postoperative seizure outcomes. This study enrolled 39 TLE patients with 29 healthy controls (HCs). Patients were divided into seizure-free (SF) and non-seizure-free (NSF) groups. Each individual network was divided into four-tier subnets. FNCS was significantly lower in the 1st level (p = 0.007/p = 0.009), 2nd level (p = 0.002/p < 0.001), and 3rd level disconnect networks (p < 0.001/p < 0.001) in SF and NSF(27.0 ± 8.4, 16 men; 30.9 ± 10.9, 8 men). SNCS was significantly lower in SF than HCs (33.3 ± 8.9, 15 men) and NSF (p = 0.008/p = 0.037) in the 1st level disconnected network. Further, significant SFC in the 1st level (p = 0.026/R²=0.169), 2nd level (p = 0.002/R²=0.283), and 3rd level disconnected networks (p = 0.011/R²=0.213) in HCs. Meanwhile, the SF group showed a positive SFC at the 2nd level disconnected network (p = 0.039/R²=0.159). Our study demonstrated that the consistency in SNCS and FNCS of the regions indirectly affected by surgery is associated with lower risk of post-surgical seizure. Preoperative multimodal magnetic resonance imaging may serve as an important tool to guide surgical planning.
Molecular characterization of a novel fusarivirus infecting the plant-pathogenic fungus Nigrospora sphaerica
Authors: Lu Xun , Lu Jianhua , Peng Shuming , Xia Qingzhu , Li Le , Long Binqing , Liu Hong 
Published: August 2024

Abstract

Here, we describe a novel mycovirus, tentatively designated as “Nigrospora sphaerica fusarivirus 2”(NsFV2), which was isolated from the phytopathogenic fungus Nigrospora sphaerica strain HNXX-Ns20. NsFV2 has a single-stranded positive-sense RNA (+ ssRNA) genome of 6,156 nucleotides, excluding the poly(A) tail, and contains two putative open reading frames (ORFs). The ORF1 encodes a large polypeptide of 1,509 amino acid (aa) containing a conserved RNA-dependent RNA polymerase (RdRp) domains and a viral helicase domain. The ORF1-encoded polypeptide shares 29.40-68.48% sequence identity with other fusariviruses and shares the highest sequence identity (68.48%) with Nigrospora sphaerica fusarivirus 1 (NsFV1) .The small ORF2 encodes a polypeptide with 483 a and contains a conserved chromosome segregation ATPase (Smc) domain. Multiple sequence alignments and phylogenetic analysis based on the ORF1-encoded polypetide indicated that NsFV2 should be new member of the genus Alphafusarivirus of family Fusariviridae.
Growth and survival of three hatchery-bred giant clams (Tridacna derasa, T. squmosa, and T. crocea) in an ocean nursery: a pilot of restocking study in China
Authors: Li Jun , Zhao Zhen , Guo Shuming , Ma Haitao , Qin Yanpin , Yu Ziniu , Zhang Yuehuan 
Published: August 2024

Abstract

Giant clams are threatened by overexploitation and global climate change (e.g., global warming and ocean acidification) throughout their wide geographical range in the Indo-Pacific. Restocking of juvenile giant clams in coral reefs could be an important strategy to restore their populations. In the present study, three species two-year-old giant clams, including Tridacna derasa (shell length: 97.9 ± 13.2 mm), T. squamosa (96.2 ± 17.4 mm), and T. crocea (44.5 ± 7.6 mm), were firstly released to two coral reefs (Wuzhizhou Island and Luhuitou) to compare their performance for one year’s duration in Southern China. No significant difference was found in survival rates among species of giant clams, while their growth of giant clams varied greatly between reef sites with better performance in Wuzhizhou Island than in Luhuitou during 12 months. Among three giant clams, T. derasa had the fastest growth at a rate of 4.45 ± 0.36 and 3.38 ± 0.32 mm month-1 in Wuzhizhou Island and Luhuitou, respectively, and their survival rates ranged from 41.04 ± 1.63% to 44.00 ± 0.82% in Wuzhizhou Island and from 30.69 ± 1.89% to 37.67 ± 4.19% in Luhuitou. Turbidity was the main factor affecting the performance of giant clams, whereas typhoons can cause mass mortality of giant clams. By restocking giant clams in the natural coral reefs, their populations can be restored, which is beneficial to the stability of coral reef ecosystems.
Leopard spot retinopathy represents the initial sign of rhegmatogenous retinal detachment in children: a report of two cases
Authors: Chen Boya , Duan Jialiang 
Published: August 2024

Abstract

Background: Leopard spots can appear in a variety of diseases; however, they are extremely rare in children with rhegmatogenous retinal detachment. This study presents two such rare cases in which leopard spot retinopathy was the initial manifestation of rhegmatogenous retinal detachment.
Case Presentation: Case 1 involved a 4-year-old boy had previously been diagnosed with left eye uveitis and received systemic steroid therapy at a local hospital, but symptoms persisted. Extensive leopard-spot changes were observed in the posterior pole of the left eye and optical coherence tomography (OCT) showed multifocal, shallow retinal detachment in the left eye. Case 2 involved a16-year-old girl who visited for decreased vision in her right eye. Examination of the right eye revealed leopard-spot changes at the posterior pole and a retinal hole in the fundus; multifocal retinal detachment was noted on OCT. In both cases, the retinas were successfully reattached after retinal laser photocoagulation or scleral buckling, with no recurrence of retinal detachment at late follow-up.
Conclusions: When leopard-spot changes are observed, the fundus must be carefully examined for any potential retinal holes, especially in pediatric patients.
A Comparison of Machine Learning Models for Mucopolysaccharidosis Early Diagnosis: A Retrospective Cohort Study using UAE SEHA Electronic Medical Records
Authors: AlShehhi Aamna , Alblooshi Hiba , Fadul Ruba , Tumzghi Natnael , Tenaiji Amal Al , Harbi Mariam Al , Al-Jasmi Fatma 
Published: August 2024

Abstract

Rare diseases, such as Mucopolysaccharidosis (MPS), present unique challenges to the healthcare system. Some of the most critical challenges are the delay and the lack of accurate disease diagnosis. Early diagnosis of MPS is crucial, as it has the potential to significantly improve patients' response to treatment, thereby reducing the risk of complications or death. This study aims to compare the performance of different machine learning (ML) models for MPS diagnosis using electronic health records (EHR) from the Abu Dhabi Health Services Company (SEHA). Our retrospective cohort study consists of 115 registered patients whose age <= 19 Years old from 2004 to 2022. Using nested cross-validation, we trained different feature extraction algorithms in combination with various ML algorithms, and we evaluated them using various evaluation metrics. Finally, the models with the highest performance were further interpreted using Shapley additive explanations (SHAP). We found that Naive Bayes trained on the domain expert features reported the highest performance: accuracy: 0.93 (0.08), AUC: 0.96(0.04), F1-score: 0.91(0.1), and MCC: 0.86 (0.16). The top reported features were acute pharyngitis, accretions on teeth, and body mass index pediatric, greater than or equal to the 95th percentile for age. This study offers a cost-effective screening method for MPS patients using non-invasive EHR.
The effect of psychological empowerment program on clinical competence and burnout of nurses working in intensive care units
Authors: Moghaddasi Jaefar , Nemati Shahnaz , Raeisi Hadi , khabazi malihe , Shirvani fatemeh 
Published: August 2024

Abstract

Introduction and aim: Burnout, perceived stresses and threats in the workplace are a global phenomenon in nursing. Meanwhile, the psychological ability of nurses through increasing their efficiency, job motivation and creative behaviors can play an important role in increasing their efficiency in therapeutic settings. Therefore, due to the high rate of burnout in intensive care unit nurses, the present study was conducted to investigate the effect of psychological empowerment program on clinical competence and burnout of intensive care unit nurses in Shahrekord teaching hospitals.
Methods: In this quasi-experimental study that was performed on 61 ICU nurses of teaching hospitals in Shahrekord, the samples were selected by convenience sampling method and randomly assigned to experimental and control groups. In the next stage, for the intervention group, the workshop and psychological empowerment sessions were implemented for eight sessions of 90 minutes and the control group only received the routine education. At the end of each session, the booklet was given to the members of the intervention group. CIRN and Maslach Burnout Questionnaire was completed for both groups before, immediately and one month after the intervention. Data has been analysed by SPSS 21 softwaer using descriptive analytic tests.
Results: The results showed that the mean of clinical competence before the intervention in the intervention group was (161. 00 ± 11.34) and in the control group was (154.53 ± 41.64) which were not significantly different (P = 0.51). The mean score of clinical competence immediately after the intervention in the intervention group was (176.68 ± 23.87) and in the control group was (160.27 ± 39.48) that no significant difference was observed between the two groups (P = 0.13). Also, the mean score of clinical competence one month after the intervention was (181.35 ± 26.99) and in the control group was (153.13± 41.10), which was a significant difference (P<0.001). Burnout before the intervention in intervention group was (52.70 ± 15.17) and in the control group was (45.07 ± 16.74), which were not significantly different (P = 0.08).
Conclusion: According to the results of a recent study, the implementation of empowerment training program is effective in the clinical performance of nurses and improves performance in the level of service to patients and treatment teams. Psychological empowerment of nurses More interventions should be done.
Defining a quantum active particle using a non-unitary quantum walk
Authors: Yamagishi Manami , Hatano Naomichi , Obuse Hideaki 
Published: August 2024

Abstract

The main aim of the present paper is to define an active particle in a quantum framework as a minimal model of quantum active matter and investigate difference and commonalities of quantum and classical active matters. Although the research field of active matter has been expanding wider and wider, most research is conducted in classical systems. We here propose a truly deterministic quantum active-particle model with a non-unitary quantum walk as minimal models of quantum active matter. We aim to reproduce similar results obtained previously with classical active Brownian particle; that is, a Brownian particle, with a finite energy take-up, becomes active and climbs up a potential wall. We realize such a system with non-unitary quantum walks. We introduce new internal states, the ground state |G⟩ and the excited state |E⟩, and a new non-unitary operator N(g) for an asymmetric transition between |G⟩ and |E⟩. The non-Hermiticity parameter g promotes transition to the excited state and hence the particle takes up energy from the environment. With our quantum active particle, we successfully observe that the movement of the quantum walker becomes more active in a non-trivial way as we increase the non-Hermiticity parameter g, which is similar to the classical active Brownian particle. Meanwhile, we also observe three unique features of quantum walks, namely, ballistic propagation of peaks in one dimension, the walker staying on the constant energy plane in two dimensions, and oscillations originating from the resonant transition between the ground state |G⟩ and excited state |E⟩ both in one and two dimensions.