bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of G and P genotypes of bovine group A rotavirus with emergence of unusual G- and P-type combinations from neonatal calf diarrhea in Kashmir, India
Authors: Hassan M.N. , Shah Iqra Hussain , Farooq S. , Wani S.A. , Qureshi S. 
Published: August 2024

AbstractA total of 490 diarrhoeic samples from calves aged between 0 and 6 months were screened for the presence of different G- and P-genotypes of rotavirus circulating in bovines in the Kashmir Valley. Of the 490 diarrhoeic samples, Group A rotavirus was detected in 68 (13.87%) samples by polymerase chain reaction (PCR) followed by RNA-PAGE. Genotyping analysis revealed G10, G6, G3, P[11] and P[5] to be the predominant types. The most common types of combinations detected were G10P[11] (27.90%) and G6P[11] (20.60%). The prevalence rate of G10 and P[11] decreased from 60% to 36.76% and 100%–69.11%, respectively. Genotypes G6, G3, P[1] and P[5], which were not previously reported, were detected and unusual combinations such as G6P[11], G3P[11], G10P[5], G3P[5], G6P[1], G6P[5], G6+G8P[11] were also observed for the first time. Fluctuations in the predominant types, emergence of new types and possible genetic reassortment events suggest an unstable epidemiological situation and the need for continuous surveillance of the circulating types to ensure the suitability of the vaccination programme. The present data suggests G10, G6, P[11] and P[5] genotypes could be incorporated in the polyvalent vaccine to offer increased protection against bovine rotavirus infection in India.
Residue dynamics, decontamination, and dietary risk assessment of pyriproxyfen and fenpropathrin in okra fruits
Authors: Sharma Pooja , Ahlawat Sushil , Agroiya Pooja , Chauhan Reena , Kumari Nisha , Yadav Surender Singh , Singh Gajender 
Published: August 2024

AbstractSumiprempt containing Pyriproxyfen and fenpropathrin insecticides is used for pest control in okra crop. Pyriproxyfen can disrupt endocrine function, while fenpropathrin disrupts voltage-gated sodium channels in neurons. Based on their health risk to consumers and impact on environmental deposition, an experiment under field was planned to evaluate deposition, decontamination and related dietary risks. A methodology was developed and validated to identify and quantify the pyriproxyfen + fenpropathrin residues, extracted via QuEChERS method using GC-MS/MS. The foliar application of ready-mix formulation (Pyriproxyfen 5% + Fenpropathrin 15% EC) @ 750 gm mL−1 ha−1 and 1,500 gm mL−1 ha−1 leads average initial deposition pyriproxyfen 5% (0.359, 0.584 ppm) and fenpropathrin (0.643, 1.005 ppm) at respective doses. Residues reached below limit of quantification (0.01 ppm) on 15th and 20th day for pyriproxyfen and 10th and 15th day for fenpropathanin at the recommended and double the recommended doses, respectively. Dissipation followed the first-order kinetics with half-life value ranged in between 1.79–1.84 (pyriproxyfen) and 2.27–2.31 days (fenpropathrin) at the respective doses. The pre-harvest interval of 12 days was recommended for safe consumption of okra fruit. Even though all the decontamination treatments were successful in lowering the residues of both chemicals to substantial levels, washing followed by boiling was found to be the most effective treatment for reducing the residues. The risk quotient values were less than 0.01, implying that pyriproxyfen and fenpropathrin offer no dietary risk to consumers.
Effect of Maternal Iodine Excess during Pregnancy on Neonatal Thyroid Function and Neurodevelopmental Status at 12 Weeks
Authors: K Rao Deepashree , Jindal Ankur , Dabas Aashima , Sait Haseena , Yadav Sangeeta , Kapoor Seema 
Published: August 2024

Objective. This study aims to determine the effect of iodine excess in pregnant mothers on thyroid function, growth and neurodevelopment in the neonates when assessed at 12 weeks of age.
Methodology. This prospective study enrolled term neonates with birth weight >2500 gm of mothers having urine iodine concentration (UIC) ≥500 µg/L documented in the third trimester of the peripartum period. Neonatal TSH was collected by heel prick on dried blood spots within 24-72 hours of age and measured by time-resolved fluroimmunoassay. Neonates with TSH ≥11 mIU/L at birth were followed up at 2 and 12 weeks to monitor thyroid dysfunction, growth and development.
Results. A total of 2354 (n = 1575 in the delivery room) maternal urine samples were collected of which 598 (25.4%) had elevated UIC. Forty-nine (12.2%) neonates had TSH ≥11mIU/L on newborn screening of whom 18 and 3 neonates had residual elevated TSH at 2 and 12 weeks of life, respectively. Maternal iodine levels correlated weakly with TSH at 2 weeks (rho = 0.299; p = 0.037). No child required treatment for congenital hypothyroidism. Eight babies additionally had TSH >5 mIU/L at 12 weeks of life. The growth and development of babies with or without TSH elevation was comparable at three months (p >0.05).
Conclusion. Maternal iodine excess in pregnancy and peripartum period causes transient hyperthyrotropinemia in neonates that did not affect the growth and development at 3 months of age.
Association of Genetically Predicted Skipping of COL4A4 Exon 27 with Hematuria and Albuminuria
Authors: Lona-Durazo Frida , Omachi Kohei , Fermin Damian , Eichinger Felix , Troost Jonathan P. , Lin Meei-Hua , Dinsmore Ian R. , Mirshahi Tooraj , Chang Alexander R. , Miner Jeffrey H. , Paterson Andrew D. , Barua Moumita , Gagliano Taliun Sarah A. 
Published: August 2024

Key Points

Using transcriptome-wide association studies, we identified an association between splicing out of exon 27 of COL4A4 and hematuria.We confirmed the presence of COL4A4 exon 27 splicing in an independent cohort.Functional assays revealed that the COL4A4 transcript with exon 27 spliced out affects collagen IV trimer assembly and secretion.

Background
Hematuria is an established sign of glomerular disease and can be associated with kidney failure, but there has been limited scientific study of this trait.

Methods
Here, we combined genetic data from the UK Biobank with predicted gene expression and splicing from Genotype Tissue Expression kidney cortex samples (n=65) in a transcriptome-wide association study to identify additional potential biological mechanisms influencing hematuria.

Results
The transcriptome-wide association study using kidney cortex identified significant associations for five genes in expression and three significant splicing events. Notably, we identified an association between the skipping of COL4A4 exon 27, which is genetically predicted by intronic rs11898094 (minor allele frequency 13%), and hematuria. Association between this variant was also found with urinary albumin excretion. We found independent evidence supporting the same variant predicting this skipping event in glomeruli-derived mRNA transcriptomics data (n=245) from the Nephrotic Syndrome Study Network. The functional significance of loss of exon 27 was demonstrated using the split NanoLuc-based α3α4α5(IV) heterotrimer assay, in which type IV collagen heterotrimer formation was quantified by luminescence. The causal splicing variant for this skipping event is yet to be identified.

Conclusions
In summary, by integrating multiple data types, we identified a potential splicing event associated with hematuria and albuminuria.
Vulnerabilidade e sofrimento psíquico: condições de acesso em saúde durante o pré-natal
Authors: Das Neves Juliana Bittencourt , Yavo Ivete de Souza 
Published: August 2024

INTRODUÇÃO: Observa-se nas últimas décadas a necessidade cada vez maior de repensarmos os modelos de atenção à saúde, bem como a criação de políticas públicas efetivas para parcelas significativas da população brasileira em condições de maior vulnerabilidade, visando assim melhoria de oferta de produtos e serviços que favoreçam melhora na qualidade de vida especialmente no âmbito da saúde. Tal demanda é reforçada principalmente quando atravessada pelo racismo estrutural que circunda o acesso à assistência em saúde da mulher negra. OBJETIVO: Desta forma, o presente estudo pretendeu compreender a vivência psicológica de mulheres gestantes frente ao atendimento que receberam durante o pré-natal, bem como conhecer possíveis fatores geradores de sofrimento psíquico. MÉTODO: Para tal, foi realizada uma pesquisa de caráter qualitativo através de um questionário com questões semi abertas e um questionário sócio-demográfico. A amostra foi composta por seis mulheres. CONSIDERAÇÕES FINAIS: Os dados foram compreendidos à luz da teoria psicanalítica associada a teóricos que abordam questões transversais em saúde.
Reference 1D Seismic Velocity Models for Volcano Monitoring and Imaging: Methods, Models, and Applications
Authors: Pesicek Jeremy D. , Ryberg Trond 
Published: August 2024

Abstract
Seismic velocity models of the crust are an integral part of earthquake monitoring systems at volcanoes. 1D models that vary only in depth are typically used for real-time hypocenter determination and serve as critical reference models for detailed 3D imaging studies and geomechanical modeling. Such models are usually computed using seismic tomographic methods that rely on P- and S-wave arrival-time picks from numerous earthquakes recorded at receivers around the volcano. Traditional linearized tomographic methods that jointly invert for source locations, velocity structure, and station corrections depend critically on having reasonable starting values for the unknown parameters, are susceptible to local misfit minima and divergence, and often do not provide adequate uncertainty information. These issues are often exacerbated by sparse seismic networks, inadequate distributions of seismicity, and/or poor data quality common at volcanoes. In contrast, modern probabilistic global search methods avoid these issues only at the cost of increased computation time. In this article, we review both approaches and present example applications and comparisons at several volcanoes in the United States, including Mount Hood (Oregon), Mount St. Helens (Washington), the Island of Hawai’i, and Mount Cleveland (Alaska). We provide guidance on the proper usage of these methods as relevant to challenges specific to volcano monitoring and imaging. Finally, we survey-published 1D P-wave velocity models from around the world and use them to derive a generic stratovolcano velocity model, which serves as a useful reference model for comparison and when local velocity information is sparse.
Giant congenital melanocytic nevus in an 8-year-old female: a case report
Authors: Mariel Sánchez Guevara , Miriam Hidalgo Ostoa , María Elena García Campos , Iiliana Soto Piña Pamela 
Published: August 2024

Giant congenital melanocytic nevus are melanocytic lesions secondary to abnormal melanoblast migration during the embryogenesis. The incidence reported is in 1 case for every 20 000 newborns and it is present since birth. The importance of its detection relies on the associated complications, such as melanoma development or neurocutaneous melanosis, as well as the psychological and social impact they generate; patients will require a long-term multidisciplinary attention. There is no elective treatment, management is still controversial, it must be individualized according to the characteristics of the nevus and the age of the patient. We present the case of an 8-year-old patient where the diagnosis of giant congenital melanocytic nevus is determined for the first time.
Manifestation of ocular tuberculosis in a north Indian tertiary care centre: Case series
Authors: Singh Neha , Singh Praveen 
Published: August 2024

Ocular tuberculosis acts as a challenge throughout the world, and its pathophysiology, diagnosis, and treatment are a topic for debate. The present case series represents retrospective study focusing on the variable clinical presentations, the diagnostic and therapeutic characteristics of ocular tuberculosis. We report 12 patients of ocular tuberculosis with variable presentations. : Retrospective analysis of records of patients diagnosed with ocular tuberculosis from August 2022 till March 2023 was done. Diagnosis of ocular tuberculosis was based on detailed ocular and systemic examination including best corrected visual acuity (BCVA), Slit lamp examination, fundus examination, OCT. The patients presenting with features suggestive of ocular tuberculosis were further investigated with complete blood count (CBC), erythrocyte sedimentation rate (ESR), Monteaux test, Chest X-ray, Cartridge Based Nuclear Amplification Test (CBNAAT) and rheumatological work up. Out of 12 of ocular tuberculosis patients, 4 were diagnosed with active retinal vasculitis, 3with choroiditis, 3 had panuveitis, and remaining 2 patients had unilateral nodular episcleritis. The diagnosis was based on compatible clinical picture, highly positive Tuberculin skin test and dramatic response to anti-tuberculous drugs. All 12 cases were strongly Mantaux test positive (2 developed injection site ulceration). All cases were started on ATT immediately with pulmonologist consultation and showed clinical improvement after 1 month. Ocular tuberculosis can have myriad presentations. Positive Mantaux test plays an important role in supporting diagnosis of ocular tuberculosis.
A spectrophotometric analysis of copper and zinc released from stainless steel brackets coated with a combination of copper oxide and zinc oxide nanoparticles in artificial saliva
Authors: Duraisamy Sangeetha , Anandan Nirmala , Patil Digvijay 
Published: August 2024

Nanocoating stainless steel orthodontic brackets with a combination of copper and zinc oxide nanoparticles might alter the quantity of ions released from them in saliva. The purpose of the study was to evaluate the quantity of copper and zinc ions released from stainless steel brackets coated with a combination of copper oxide and zinc oxide. Stainless steel orthodontic brackets (Ormco Mini -Diamond series 0.22” slot, MBT prescription) (ORMCO CORP Glendora, California, USA) (n= 15 in each group) were coated with nanoparticles of copper oxide (Group I) , zinc oxide (Group II) and a combination of copper oxide –zinc oxide (Group III) nanoparticles using a spray pyrolysis method . The quantity of copper and zinc ions released from these three groups of brackets, when stored in artificial saliva and intubated at 37 C was evaluated at 24hrs, 7th day, 14th day and 28th day using an atomic absorption spectrometer. The three groups of coated brackets released significantly more copper and zinc ions than the uncoated brackets. The copper oxide nanocoated and zinc oxide nano coated stainless steel orthodontic brackets released more copper and zinc ions when compared to the copper oxide - zinc oxide combination nanocoated orthodontic brackets and uncoated brackets. The highest surge of ion release was noted at the 7th day in all the three coated groups for both the ions evaluated. Brackets coated with a combination of copper oxide and zinc oxide nanoparticles demonstrated reduced levels of copper and zinc ion release in artificial saliva when compared to copper oxide nanocoated brackets and zinc oxide nanocoated brackets..
Stability of skeletal open bite correction in adult patients by molar intrusion: A meta analysis
Authors: Prakash V. S.  , Sharma Anil , Verma Santosh , Chauhan Arun Kumar 
Published: August 2024

To review and compile literature to check the post retention stability of skeletal open bite correction by molar intrusion. Articles were searched in Electronic database of English literature i.e. PubMed, Cochrane Library, Embase and google scholar. Titles and abstracts of potential articles with inclusion criteria were examined and included based on consensus agreement. Abstracts of articles having characteristics that were uncertain were examined, with full article retrieval if necessary. The reference list of the retrieved articles were also searched. On comparing the mean post treatment and post retention of overbite derived from their respected forest plot graphs, there was 32 percent relapse in the overbite correction. This study states that skeletal open bite patients treated with molar intrusion have 68 % stability, whereas the intruded molar have a 95% stability.
Perubahan Pekerjaan Beton Lining in Situ ke Beton Precast pada Jaringan Irigasi D.I. Tapin Kabupaten Tapin
Authors: Darmawan Hafiz 
Published: August 2024

Penilitian ini bertujuan untuk mengevaluasi dan menganalisis perubahan metode pada lining beton pada saluran irigasi, antara beton insitu dan beton Precast. Dengan dilakukannya evaluasi terhadap perubahan metode pekerjaan tersebut akhirnya didapatkan bahwa metode dengan menggunakan beton Precast memiliki beberapa kelebihan dibandingkan beton insitu. Dari segi mutu pelaksanaan, mutu beton menjadi mutu K-225 yang semula hanya beton mutu K-175. Selain itu beton Precast bisa meminimalkan kehilangan/material losses dalam waktu pelaksanaan karena dicetak pada bidang yang presisi, dan waktu pelaksanaan yang secara signifikan bisa lebih cepat. Pada Analisis biaya penggunaan beton Precast mengalami kenaikan harga yang diakibatkan oleh kenaikan mutu beton, langsir, dan penambahan dowel bar. Namun dari hasil analisis terlihat juga bahwa semakin banyak volume beton Precast maka biaya konstruksi bisa semakin turun.
Penggunaan Beton Plastis pada Dinding Halang Tubuh Bendung Proyek Bendungan Jlantah
Authors: Adhi Bagas Wahyu , Markawie Markawie 
Published: August 2024

Pondasi merupakan bagian yang sangat penting bagi suatu struktur tak terkecuali untuk bangunan air seperti bendungan. Perbaikan pondasi bendungan bertujuan agar tegangan tanah yang timbul sebagai akibat berat sendiri dari bendungan, tekanan air, gaya gempa, dan muatan -muatan yang bekerja tidak melebihi daya dukung tanah pondasi bendungan di bagian bawah maupun di tebing kiri dan kanan bendungan. Pada Proyek Pembangunan Bendungan Jlantah terdapat permasalahan berkaitan dengan pondasi bendungan. Perbaikan fondasi Bendungan Jlantah semula didesain menggunakan grouting tirai, tetapi dari hasil uji grouting pada bagian palung sungai hasilnya tidak dapat mencapai nilai kekedapan air (Lugeon) yang disyaratkan. Mengingat endapan alluvial dan lapisan batuan yang lapuk kuat masih cukup porus, maka diperlukan perbaikan pondasi secara khusus menggunakan dinding halang (cut-off wall atau diaphragma wall).Tujuan dari penggunaan dinding halang / Diaphragm wall adalah sebagai perkuatan pondasi pada tubuh bending. Metode yang digunakan dalam pembuatan beton plastis adalah trial eror di laboratorium untuk mendapatkan hasil yang sesuai dengan standar. Kesimpulan yang diperoleh dari beton plastis pada dinding halang adalah sudah sesuai dengan standar yang berlaku.
The Growth Dynamics of King Grass (Pennisetum purpureophoides) in The Application of Beef Cattle Dung Enriched Azolla microphylla
Authors: Hidayat Nur , Prasetyo Prasetyo , Haryoko Imbang , Setiyaningrum Agustinah , Harwanto Harwanto , Ulfah Annistia Rahmadian 
Published: August 2024

Research on the cultivation of king grass (Pennisetum purpureophoides) was conducted to obtain information on growth, productivity, and continuity in providing forage for a sustainablee ruminant farming business. One of the cultivation factors is fertilization using beef cow manure as a renewable, inexhaustible natural resource or organic fertilizer. Improving the quality beef manure is important for better stimulation of plant growth and production. Fertilizer can be enriched with Azolla microphylla as a good nitrogen contributor. This study used beef cow dung fertilizer at doses of 10, 20, 30 tons per hectare per defoliation, and Azolla microphylla enrichment by 0%, 10%, and 20 % as treatments in a completely randomized design (CRD). The observed variables were plant growth (plant height, stem diameter, number of plants, and number of leaves as production), every 14 days until harvesting on day 42. The data obtained were analyzed descriptively based on the dynamics of plant growth. The result showed that the growth dynamics of the king grass were strongly influenced by fertilizer and plant’s age but no interaction each other. In conclusion fertilizer made of beef cattle dung enriched with Azolla microphylla is a feasible nitrogen source. The optimum results were obtained from using 30 tons beef cattle dung enriched with 20% Azolla microphylla to fertilize per hectare per defoliation soil.
Evaluation of Onion Peel as Feed Additive on Performance Production Broiler
Authors: Nurul Jannah Saadatin , Edhy Sudjarwo  , Osfar Sjofjan  
Published: August 2024

This study evaluated the impact of adding onion (Allium cepa L.), shallot (Allium ascalonicum L.), and garlic (Allium sativum L.) peels as additives to the broiler production. The material used was 189 one-day-old commercial broiler chicken Lohmann strain (unsexing) and assigned to nine treatments and three replications: T0(-): basal diet, T0(+): basal diet + antibiotic (zinc bacitracin 0.1%), T1: basal diet + 0.5% garlic peel, T2: basal diet + 0.5% shallot peel, T3: basal diet + 0.5% onion peel, T4: basal diet + 0.25% garlic peel and 0.25% shallot peel, T5: basal diet + 0.25%. garlic peel + 0.25% onion peel, T6: basal diet + 0.25% shallot peel + 0.25% onion peel, T7: basal diet + 0.167% garlic peel + 0.167% shallot peel + 0.167% onion peel. Feed intake, final body weight, feed conversion ratio (FCR), mortality, productivity index, and income over feed cost were the variables that were observed in the current study. The data were analysed using Analysis of Variance (ANOVA). The results of the study show that the addition of the three garlic powders do not give any effect (P>0.05) on feed intake, final body weight, FCR, mortality, production index and IOFC. The conclusions of this study showed that garlic peel, shallot peel, and onion peel could be used as feed additive. This choice yields positive effects on production performance, particularly in terms of Feed Conversion Ratio (FCR) and the production index.
Media Sosial dan Aktivisme Digital Perempuan: Analisis Wacana #Ibutunggalmelawan di Instagram
Authors: Dwityas Nindyta Aisyah , Marta Rustono Farady , Briandana Rizki 
Published: August 2024

Digital activism, commonly referred to as online activism, is the practice of using electronic communication tools as a type of activism aimed at fostering the development of community movements. Digital activism is connected to online discourse through various mechanisms, including social media platforms, connectivity actions and the discursive construction of digital technology. The purpose of this study is to examine the discourse that is growing in the comments section of the online petition content for the #MotherSingleMelawan on the Instagram account @changeorg_id, as well as the signs of female digital activism that are emerging. Marie-Anne Paveau pioneered the qualitative discourse analysis research methodology, which is particularly relevant when applied to online communication. The results of the research show that women's digital activism in the comments column of the #SingleMotherMelawan online petition consists of four main discourse themes, namely; 1) the discourse of belief-based gender oppression, 2) the discourse of gender relations in parental relations, 3) the discourse of justice for victims of gender-based violence, 4) the discourse of discrimination against single mothers.