bluuebunny/crossref_metadata_2025_split huggingface dataset:
NAD+ Metabolic Enzyme Inhibitor as Radiosensitizer for Malignant Meningioma and its Modulation of P53 Expression
Authors: Lv Yifan , Deng Yuxuan , Feng Jie , Liu Jinqiu , Yang Mingxu , Pu Zhuonan , Zhang Shaodong , Wu Zhen , Ji Nan , Park Deric M. , Hao Shuyu 
Published: July 2024

Abstract
Surgical resection followed by radiotherapy (RT) is recommended for malignant meningioma, but poor outcome is unavoidable. To improve the efficacy of RT in malignant meningioma, a targeted radiosensitizer can be added. Nicotinamide phosphoribosyltransferase (NAMPT), highly expressed in high-grade meningiomas, may play a role in determining the radioresponse. Herein, we evaluated the impact of NAMPT inhibition on radiosensitivity in malignant meningioma in vivo and in vitro. IOMM-Lee and TTMM705 cells were treated with NAMPT inhibition (FK866 or shRNA NAMPT) before irradiation. The subsequent clonogenic assay demonstrated significantly increased radiosensitivity. Combination treatment with FK866 and irradiation significantly increased the number of G2/M-phase cells, percentage of apoptotic cells, and γ-H2A.X level compared with FK866 or RT alone. We examined the effect of NAMPT inhibition on NMI and p53 expression in IOMM-Lee and TTMM705 cells. NAMPT inhibition by FK866 and shRNA treatment increased NMI, p53, CDKN1A and BAX expression. Additionally, we assessed the efficacy of FK866/RT combination treatment in vivo. The combination treatment exhibited increased antitumor efficacy compared with either treatment alone. The Ki67 level was significantly lower, and the p53 and γ-H2A.X levels were significantly higher in the combination treatment group than in the other three groups. In conclusion, these results indicate that FK866 improves radiosensitivity in malignant meningioma, an effect that may be attributed to the increase in p53 expression.
Acetalax (Oxyphenisatin Acetate, NSC 59687) and Bisacodyl Cause Oncosis in Triple-Negative Breast Cancer Cell Lines by Poisoning the Ion Exchange Membrane Protein TRPM4
Authors: Mizunuma Makito , Redon Christophe E. , Saha Liton Kumar , Tran Andy D. , Dhall Anjali , Sebastian Robin , Taniyama Daiki , Kruhlak Michael J. , Reinhold William C. , Takebe Naoko , Pommier Yves 
Published: July 2024

Abstract

Triple-negative breast cancer (TNBC) is clinically aggressive and relatively unresponsive to current therapies. Therefore, the development of new anticancer agents is needed to satisfy clinical needs. Oxyphenisatin acetate (Acetalax), which had been used as a laxative, has recently been reported to have anticancer activity in murine models. In this study, we demonstrate that Acetalax and its diphenolic laxative structural analogue bisacodyl (Dulcolax) exhibit potent antiproliferative activity in TNBC cell lines and cause oncosis, a nonapoptotic cell death characterized by cellular and nuclear swelling and cell membrane blebbing, leading to mitochondrial dysfunction, ATP depletion, and enhanced immune and inflammatory responses. Mechanistically, we provide evidence that transient receptor potential melastatin member 4 (TRPM4) is poisoned by Acetalax and bisacodyl in MDA-MB468, BT549, and HS578T TNBC cells. MDA-MB231 and MDA-MB436 TNBC cells without endogenous TRPM4 expression as well as TRPM4-knockout TNBC cells were found to be Acetalax- and bisacodyl-resistant. Conversely, ectopic expression of TRPM4 sensitized MDA-MB231 and MDA-MB436 cells to Acetalax. TRPM4 was also lost in cells with acquired Acetalax resistance. Moreover, TRPM4 is rapidly degraded by the ubiquitin–proteasome system upon acute exposure to Acetalax and bisacodyl. Together, these results demonstrate that TRPM4 is a previously unknown target of Acetalax and bisacodyl and that TRPM4 expression in cancer cells is a predictor of Acetalax and bisacodyl efficacy and could be used for the clinical development of these drugs as anticancer agents.

Significance:
Acetalax and bisacodyl kill cancer cells by causing oncosis following poisoning of the plasma membrane sodium transporter TRPM4 and represent a new therapeutic approach for TNBC.
Inflammation‐Derived and Clinical Indicator‐Based Predictive Model for Ischemic Stroke Recovery
Authors: Luo Jiao , Cai You , Xiao Peng , Cao Changchun , Huang Meiling , Zhang Xiaohua , Guo Jie , Huo Yongyang , Tang Qiaoyan , Zhao Liuyang , Liu Jiabang , Ma Yaqi , Yang Anqun , Zhou Mingchao , Wang Yulong 
Published: July 2024

Background
Neuroinflammatory responses are closely associated with poststroke prognosis severity. This study aimed to develop a predictive model, combining inflammation‐derived markers and clinical indicators, for distinguishing functional outcomes in patients with subacute ischemic stroke.

Methods and Results
Based on activities of daily living assessments, ischemic stroke participants were categorized into groups with little effective (LE) recovery and obvious effective (OE) recovery. Initial biocandidates were identified by overlapping differentially expressed proteins from proteomics of clinical serum samples (5 LE, 5 OE, and 6 healthy controls) and differentially expressed genes from an RNA sequence of the ischemic cortex in middle cerebral artery occlusion mice (n=3). Multidimensional validations were conducted in ischemia–reperfusion models and a clinical cohort (15 LE, 11 OE, and 18 healthy controls). Models of robust biocandidates combined with clinical indicators were developed with machine learning in the training data set and prediction in another test data set (15 LE and 11 OE). We identified 194 differentially expressed proteins (LE versus healthy controls) and 174 differentially expressed proteins (OE versus healthy controls) in human serum, and 5121 differentially expressed genes (day 3) and 5906 differentially expressed genes (day 7) in middle cerebral artery occlusion mice cortex. Inflammation‐derived biomarkers TIMP1 (tissue inhibitor metalloproteinase‐1) and galactosidase‐binding protein LGLAS3 (galectin‐3) exhibited robust increases under ischemic injury in mice and humans. TIMP1 and LGALS3 coupled with clinical indicators (hemoglobin, low‐density lipoprotein cholesterol, and uric acid) were developed into a combined model for differentiating functional outcome with high accuracy (area under the curve, 0.8).

Conclusions
The combined model is a valuable tool for evaluating prognostic outcomes, and the predictive factors can facilitate development of better treatment strategies.
Reduced expression of fMRI subsequent memory effects with increasing severity across the Alzheimer’s disease risk spectrum
Authors: Soch Joram , Richter Anni , Kizilirmak Jasmin M. , Schütze Hartmut , Altenstein Slawek , Dechent Peter , Fliessbach Klaus , Glanz Wenzel , Herrera Ana Lucia , Hetzer Stefan , Incesoy Enise I. , Kilimann Ingo , Kimmich Okka , Lammerding Dominik , Laske Christoph , Lohse Andrea , Lüsebrink Falk , Munk Matthias H. , Peters Oliver , Preis Lukas , Priller Josef , Rostamzadeh Ayda , Roy-Kluth Nina , Scheffler Klaus , Schneider Anja , Spottke Annika , Spruth Eike Jakob , Teipel Stefan , Wiltfang Jens , Jessen Frank , Düzel Emrah , Schott Björn H. 
Published: July 2024

Abstract
In functional magnetic resonance imaging (fMRI) studies, episodic memory is commonly investigated with the subsequent memory paradigm in which brain activity is recorded during encoding and analyzed as a function of subsequent remembering and forgetting. Impaired episodic memory is common in individuals with or at risk for Alzheimer’s disease (AD), but only few studies have reported subsequent memory effects in AD or its risk states like mild cognitive impairment (MCI). One reason for this might be that subsequent memory responses may be blunted in AD or MCI and thus less likely to manifest in fMRI signal differences. Here, we used Bayesian model selection of single-subject fMRI general linear models (GLMs) for a visual novelty and memory encoding experiment to compare the model performance of categorical and parametric subsequent memory models as well as memory-invariant models in a clinical cohort (N = 468) comprising healthy controls (HC) as well as individuals with subjective cognitive decline (SCD), MCI, and AD, plus healthy relatives of AD patients (AD-rel). We could replicate the previously reported superiority of parametric subsequent memory models over categorical models (Soch, Richter, Schütze, Kizilirmak, Assmann, Knopf, et al., 2021) in the HC and also in the SCD and AD-rel groups. However, memory-invariant models outperformed any model assuming subsequent memory effects in the MCI and AD groups. In the AD group, we additionally found substantially lower model preference for models assuming novelty compared to models not differentiating between novel and familiar stimuli. Our results suggest that voxel-wise memory-related fMRI activity patterns in AD and also MCI should be interpreted with caution and point to the need for additional or alternative approaches to investigate memory function.
Electrodiagnosis of polyneuropathy, organmegaly, endocrinopathy, M-protein, skin changes syndrome patients with peripheral neuropathy and potential-related risk factors
Authors: Niu Guanru , Liu Yuchen , Zhang Zhen , Song Siyuan , Li Tian , Shen Yijun , Dong Jihong 
Published: July 2024

Objectives: To explore the correlation between classification and electrophysiology of polyneuropathy, organmegaly, endocrinopathy, M-protein, skin changes syndrome (POEMS)-related peripheral neuropathy (PN). Methods: We analyzed the data of 30 POEMS patients admitted to Zhongshan Hospital affiliated with Fudan University between February 2017 and February 2023. The degree of PN was determined according to its classification. All three groups of patients underwent neuroelectromyography, and the nerve conduction velocity and amplitude of the three groups were analyzed. Results: The compound motor active potentials (CMAP) of the peroneal, tibial, and ulnar nerves decreased significantly with increasing disease grade, and the motor conduction velocity of the peroneal, median, and tibial nerves decreased significantly in grade 3 compared with grade 1 and 2. The action potential of sensory nerves (sensory nerve action potential) and the conduction speed of sensory impulses (sensory conduction velocity (SCV) in the sural nerve in grade 3 were significantly lower than those in grades 1 and 2. Linear regression analysis showed that there was a linear correlation between CMAP of peroneal nerve and vascular endothelial growth factor. The SCV of the ulnar nerve significantly correlated with the course of the disease. Discussion: Neuroelectromyography can effectively evaluate the degree of PN in patients with POEMS, providing a reliable reference for further clinical treatment.
Patient preferences for teleradiology services and remote image interpretation: An empirical study
Authors: Alanzi Turki M , Alanzi Nouf , Arif Wejdan M. , Alkhunaifer Alhanoof , Al Ghaseb Lamia , Albadrani Yara Hamad , Hasoosah Norah , Abdullah Ruya Adel , Al Shullah Batool 
Published: July 2024

Study purpose This study aims to examine patient preferences for teleradiology services and remote image interpretation. In this context, this study aims to address the following research questions: (i) How do patients perceive teleradiology services, focusing specifically on comfort, quality of care and satisfaction, communication and information, and accessibility and ease of use; (ii) How do patient demographics (age, gender, urban vs. rural residence) influence the perceptions on teleradiology services? Methods A cross-sectional survey design is adopted in this study. The survey comprises five sections targeting demographic information, comfort, and preferences regarding remote image interpretation, perceived quality of care and satisfaction, communication and information clarity, and accessibility and technology aspects using five-point Likert scale ratings. A total of 406 patients (209 males and 197 females; 170 urban residents; 174 semi-urban residents, and 62 rural residents) using teleradiology services participated in the study. Results Participants reported high satisfaction with remote image interpretation (3.78 ± 1.19), quality of care (3.31 ± 1.19), understanding (3.84 ± 1.43), and user-friendliness (3.67 ± 1.29). Key issues were technical problems (3.81 ± 1.35), feedback difficulties (3.19 ± 1.58), privacy concerns (2.43 ± 1.46), and low awareness (2.37 ± 1.12). Urban participants scored significantly better in comfort, preferences, and communication than those from semi-urban and rural areas. Conclusion Teleradiology design and implementation should be optimized to align with patient preferences and enhance overall satisfaction.
The performance and charge behaviour in melter/smelter for the production of hot metal in hydrogen DRI-based steelmaking
Authors: Shahabuddin M. , Rahbari Alireza , Sabah Shabnam , Brooks Geoffrey , Pye John , Rhamdhani M. Akbar 
Published: July 2024

Direct reduced iron (DRI)-based steelmaking requires high-grade iron ore (Fe > 65 wt-%). However, a swift decline in ore grade has forced steelmakers to use lower-grade ores for DRI making, creating challenges in subsequent processes dealing with high gangue content for steelmaking. Hence, an intermediate melting/smelting furnace has been proposed to treat high gangue content from the DRI before transferring hot metal into an electric arc furnace (EAF)/basic oxygen furnace (BOF) for steelmaking. This study investigates and models the performance and charge behaviour in a melter/smelter with respect to the gangue content, DRI carbon content and temperature. The results showed that the specific energy consumption (SEC) and off-gas production increase with increasing temperature, carbon in DRI and gangue content. At 90 wt-% metallisation ratio (MR) using the lowest-grade ore (gangue 16 wt-%), the SEC at 1300 °C was calculated to be 706 kWh/tHM compared to 850 kWh/tHM at 1600 °C using 3.5 wt-% carbon. The MR is predicted to profoundly impact the SEC, as the increasing MR decreases the SEC markedly. At 90 wt-% MR, the SEC using the lowest-grade ore was 805 kWh/tHM, decreasing to 684 kWh/tHM using 100 wt-%MR. Increasing MR, carbon in DRI and temperature decreases slag production consistently while increasing with the increasing gangue. Using hot DRI onsite is predicted to significantly reduce the SEC compared to cold DRI. Overall, the energy demand from cold DRI is predicted to be 120 kWh/tHM higher than hot DRI. Increasing the scrap-to-DRI ratio is predicted to decrease the energy demand, that is, every 1.0 wt-% increase in scrap decreases the SEC by 2.5 kWh/tHM.
Parameters Predictive of Propofol-Associated Acute Pancreatitis in Critically Ill Patients with COVID-19 Pneumonia: A Retrospective Cohort Study
Authors: Alabdul Razzak Iyiad , Korchemny Nikolay , Smoot Daniel , Jose Aju , Jones Allison , Price Lori Lyn , Jaber Bertrand L. , Moraco Andrew H. 
Published: July 2024

Background Propofol, a commonly used agent for short- and long-term sedation, is associated with acute pancreatitis. The main indirect mechanism of propofol-associated acute pancreatitis is by inducing hypertriglyceridemia. Patients with severe coronavirus disease 2019 (COVID-19) pneumonia often require prolonged mechanical ventilation and sedation. We examined the incidence rate of acute pancreatitis among critically ill adults with COVID-19 pneumonia on mechanical ventilation receiving propofol. In addition, we attempted to determine cutoff levels of serum triglycerides and doses of propofol that are predictive of propofol-associated acute pancreatitis. Methods This was a multicenter retrospective cohort study using a large dataset of hospitalized patients with COVID-19. The collected data included the number of days on propofol, cumulative doses of propofol, peak levels of serum triglycerides, serum lipase levels, and abdominal imaging findings. We used receiver-operating characteristic analysis in conjunction with Youden's index to identify the optimal thresholds for propofol administration parameters and levels of triglycerides that would provide maximal sensitivity and specificity for predicting acute pancreatitis. Results Out of 499 critically ill patients with COVID-19 pneumonia, 154 met the inclusion criteria. Six (4%) patients had suspected acute pancreatitis based on elevated serum lipase levels. Cutoff values greater than 688 mg/dL for peak level of triglycerides, 4.5 days on propofol, 3007 mg/day for average daily propofol dose, and 24 113 mg for cumulative propofol dose were associated with high risk of suspected acute pancreatitis. The negative predictive values for suspected acute pancreatitis using these cutoffs ranged from 98% to 100%. Conclusions Propofol use in critically ill COVID-19 patients is associated with a low incidence rate of acute pancreatitis. We identified cutoff values for serum triglycerides and cumulative propofol dose that are linked to higher risk of propofol-associated pancreatitis. More research is needed to examine the true incidence of propofol-associated pancreatitis and help develop optimal cutoff values for certain parameters to help guide safe propofol administration.
Optimization on manifold injection in DI diesel engine fuelled with acetylene
Authors: Sonachalam M. , Manieniyan V. , Senthilkumar R. 
Published: July 2024

Researchers demonstrated that implementing new combustion technology and optimising fuel quantity results in a significant reduction in traditional fossil fuel usage and emission levels. The Reactivity Controlled Compression Ignition (RCCI) combustion strategy is one of the low temperature combustion technologies, and it is used to reduce the overall combustion temperature while also providing better combustion control. This study looks into RCCI combustion technology, which uses conventional diesel fuel as the high reactivity fuel (HRF) injected through the injector and acetylene gas as the low reactivity fuel (LRF) injected into the cylinder via a modified inlet manifold alongside air. The modified engine setup was tested for performance, emissions, and combustion under various load conditions, as well as different mass flow rates of acetylene gas, a low reactivity fuel that is injected with air. The flow field of the low reactivity fuel at the inlet manifold is analysed using the Computational Fluid Dynamics principle, which is used to determine the best flow rate for improving combustion quality. According to the simulation results, the optimal acetylene flow rate is 3 Litres Per Minute (LPM), and experimentation shows that at 3 LPM acetylene injection, the brake thermal efficiency (BTE) improves by about 3.2%, and emissions such as carbon monoxide (CO), hydrocarbon (HC), smoke intensity, and oxides of nitrogen (NOx) are reduced by about 35%, 17%, 10%, and 21%, respectively.
Evaluating the performance of spherical, hemispherical, and tubular solar stills with various configurations - A detailed review
Authors: Jodah Faiz T , Alawee Wissam H , Dhahad Hayder A , Omara ZM 
Published: July 2024

Solving global water shortages has become an urgent challenge, hindering sustainable development. Therefore, comparing different solar still designs from application and economic perspectives is necessary. Solar distillation is considered a major innovation in the alternative energy sector for purifying brackish or brine water into clean water. Despite the extensive literature on improved solar stills, determining the most efficient designs for residential and industrial applications remains difficult. This review compares the productivity of spherical, hemispherical, and tubular solar still designs. The aim is to study the factors that influence the efficiency of each type and to analyze recent research and results obtained under different conditions. The results show that innovations in solar distillation design can take many forms to improve efficiency and productivity. For example, adding parabolic mirrors can increase productivity in spherical, hemispherical, and tubular stills by 35 to 70%. Likewise, innovative designs such as rotating spheres and changing bowl shapes significantly increased the productivity of spherical and hemispherical stills. Likewise, retrofitting a still with vacuum generation technology can significantly increase yields by 50 to 70%. In addition, using nanomaterials, especially nanophase change materials (NPCM), has increased the efficiency of the spherical and tubular stills by 116.5%, producing 7.62 kg/m2 per day. Therefore, the NPCM-equipped model was still the most efficient option among the three designs.
Impact of Palliative Care Referral on End-of-Life Outcomes for Patients With Hematologic Malignancy
Authors: Seecof Olivia M. , Jang Charley , Abdul Hay Maher 
Published: July 2024

Context Compared to patients with solid malignancies, less is known about the role of palliative care in patients with hematologic malignancies, leading to underutilization of palliative care. Objectives Evaluate the timing and impact of palliative care referrals on end-of-life outcomes over a 5-year period with intent to improve the utilization of palliative care in patients with advanced hematologic malignancies. Methods A retrospective cohort of patients from an urban, NCI-designated comprehensive cancer center, aged 18 years and older with a diagnosis of an advanced hematologic malignancy were separated into groups of early, late, very late, or no specialty palliative care. Logistic regression models were constructed to examine variables associated with timing of palliative care referral. Groups were compared using the Kruskal Wallis test and Dunn’s test with a Bonferroni correction method. Results 222 patients with advanced hematologic malignancies who died between July 1, 20218 and June 30, 2023 were included. 50 (23%), 41 (18%), and 51 (23%) patients received an early, late, and very late palliative care referral, respectively and 80 (36%) patients did not receive a palliative care referral. There was a significantly high completion of ACP documentation among the palliative care cohorts. There was no significant difference among all cohorts in end-of-life outcomes in the last 14 or 30 days of life. Conclusion ACP documentation improved with palliative care, however, end-of-life outcomes did not. These results are likely due to the majority of late, inpatient palliative care referrals. Future studies with targeted interventions are needed to improve these outcomes.