bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow Cytometry Analysis of Pparα Receptors in Metabolic Syndrome / Studiul Receptorilor Pparα prin Metoda Citometriei în Flux în Sindromul Metabolic
Authors: Popa Elena , Zugun-Eloae Florin , Zlei Mihaela , Jitaru Daniela , Pintilie Oana Maria , Coman Adorata Elena , Traian Maria , Ungureanu Didona Anca , Carasevici Eugen 
Published: January 2015

AbstractIntroduction. Metabolic syndrome (MS) is a cluster of distinct metabolic alterations with an increased cardiovascular risk. Peroxisome Proliferator-Activated Receptor - Alpha (PPARα), member of the nuclear receptor superfamily of transcription factors, is critically involved in the management of lipid metabolism during homeostasis or inflammatory stresses in various cell types and represents one of the therapeutic targets in MS. We analysed the PPARα expression in leukocytes of pacients with MS, in order to address PPARα involvement in these group of diseases. Material and method. Our study included 57 adult patients recruited under informed voluntary consent, investigated in order to establish whether they present MS, according to International Diabetes Federation (IDF) European guidelines and grouped in 2 lots: the MS Lot (26 patients) and control group, non-MS Lot (31 subjects). Common clinical and laboratory parameters targeted in MS evaluation were determined for all the studied cases. The expression levels of 2 molecules, PPARα and CD36 were evaluated in various circulating leukocyte populations of these patients by an optimized flow cytometry method. Statistic analysis clarifying the significance of value differences for various parameters measured was performed under SPSS and simple statistical tests (Pearson, t-Student, Chi -test). Results and discussion. The fluorescence staining for PPARα were significantly dimmer when comparing the cellular expression in eosinophils (p<0.05) of MS versus the Control group of subjects. Conclusions: Our study is the first to show that circulating eosinophils display significantly reduced PPARα protein expression in MS patients. The differences in key molecule expression in circulating leukocytes (like PPAR species, CD36, and other) might be evocatory for the endothelial dysfunction and obesity and might be of use in the therapeutic decision.
Comparing the anti-inflammatory effects of Simvastatin and Rosuvastatin by measuring IL-1β, IL-6 and TNF-α levels using a murinic caecal ligation and puncture induced sepsis model / Compararea efectelor anti-inflamatoare ale Simvastatinei și Rosuvastatinei măsurând nivelele serice ale IL-1β, IL-6 si TNF-α folosind un model de sepsis la șobolan indus prin ligatură și puncție cecală
Authors: Mărginean M Mihai , Trancă Sebastian , Ardelean-Maghiar (Mărginean) Alina , Dîrzu Dan , Huțanu Adina , Platon (Antal) Oana , Dobreanu Dan 
Published: January 2015

AbstractBackground: Sepsis is a systemic host response to an infection which may evolve into severe sepsis and septic shock. It raises many health care related concerns around the world, carrying almost 30% mortality rates and a high financial burden. The disease is characterized by the triggering of some inflammatory pathways that are ultimately proven deleterious to the host organism. Although antibiotics, fluid administration, vasopressor therapy and infectious source control remain the recommended management strategies, emerging scientific data proposes statins as a new line of treatment. These drugs were first introduced in clinical practice for their cholesterol-lowering effect but the inhibition of HMG-CoA reductase and cholesterol biosynthetic pathway exhibits some less studied effects generally referred to as pleiotropic: anti-inflammatory, antithrombotic, immunomodulatory and antioxidant properties. Objective: To asses and compare the anti-inflammatory effect of two statins - Simvastatin and Rosuvastatin - measuring blood levels of IL-1β, IL-6 and TNFα using a previously described murinic model of sepsis. Methods: We compiled four groups (C, n=7; SEP, SV, RV, n=8). Statins were administered in two doses 18 and 3 hours before surgical intervention. Sepsis was induced using the caecal ligation and puncture technique. Blood samples were obtained by venepuncture from each subject in day 1, 4, 7 and 14 (the last samples were obtained by cardiac puncture). Complete blood count, Procalcitonin, IL-1β, IL-6 and TNF-α levels were assessed. Results: White blood cell counts differed across the groups showing a higher count for the septic but untreated group. Procalcitonin reacted in all septic groups but both statin treated groups had lower levels when compared to untreated group. IL-1β levels were higher in the Rosuvastatin treated group. IL-6 levels were more heterogeneously dispersed but higher levels were noticed in the untreated septic group. The Simvastatin treated group had higher levels compared to the Rosuvastatin treated one. TNFα levels were higher in the septic untreated group and in the Rosuvastatin treated one. For the Simvastatin treated subjects, the level of TNFα was similar with the control group. Conclusion: We concluded that both drugs showed anti-inflammatory effects on the murinic CLP-induced sepsis model. Between the two, Simvastatin had greater impact by lowering blood levels of established pro-inflammatory markers.
Identification of Exonic Copy Number Variations in Dystrophin Gene Using Mlpa / Identificarea Variaţiilor Numărului de Copii în Gena Distrofinei Folosind Metoda Mlpa
Authors: Rusu Cristina , Sireteanu Adriana , Butnariu Lăcrămioara , Pânzaru Monica , Braha Elena , Mihăilă Doina , Popescu Roxana 
Published: January 2015

AbstractDuchenne and Becker muscular dystrophies (DMD/BMD) are X-linked progressive muscle disorders determined by mutations of the dystrophin (DMD) gene. Multiplex Ligation - Dependent Probe Amplification (MLPA) is a simple, inexpensive and reliable test for molecular diagnosis of DMD gene mutations. It identifies exonic copy number variations in the DMD gene, but the test should be completed with sequencing analysis in case of single exon deletions/duplications. The aim of this study was to evaluate the efficiency of MLPA as a DMD mutation screening tool in affected males and carrier females, as well as to appreciate the frequency of different types of mutations and to check the validity of the “reading frame rule”. We have used MLPA for the detection of deletions/ duplications in DMD gene in 53 individuals (30 affected males and 23 asymptomatic female relatives) referred for evaluation and genetic counseling due to the clinical suspicion of DMD/BMD. In the affected males (21 DMD and 9 BMD) MLPA had a detection rate of 63.5% (53.5% deletions and 10% duplications). The most frequently deleted exon was exon 45 and the most frequent duplication involved exons 3-5, confirming the presence of the two hotspot mutation regions reported in the literature. Mutations detected in our study have a slightly different location compared to literature data. Reading frame rule was valid in 84% of our cases.
Prognostic value of immunohistochemical expression of ZAP-70 and CD38 in chronic lymphocytic leukaemia detected on bone marrow and lymph node biopsies / Valoarea prognostică a expresiei imunohistochimice a ZAP-70 si CD38 în leucemia limfocitară cronică detectată pe biopsii osteomedulare și limfoganglionare
Authors: Fetica Bogdan , Fit Ana-Maria , Blaga Luminița , Fulop Annamaria , Pop Bogdan , Dima Delia , Cucuianu Andrei , Petrov Ljubomir 
Published: January 2015

AbstractChronic lymphocytic leukemia (CLL) has a heterogeneous clinical course. Among useful markers in identifiyng patients with poor outcome are unmutated IgVH, ZAP-70 and CD38 expression. Both ZAP-70 and CD38 were shown to be capable of identifying aggressive CLL.We analysed data from 35 patients diagnosed with CLL based on morphological and immunophenotypical criteria. In all cases peripheral blood immunophenotyping was performed as initial diagnostic test. Immunohistochemical expression of ZAP-70 and CD38 was evaluated on 21 cases of lymph node biopsies and 14 cases of bone marrow biopsies, performed at the time of diagnosis. In addition in-situ hybridization for EBER-1 was evaluated.The median age of patients was 60 years and we noted a slight male predominance. The immunophenotypic criteria (C23Prognostic information given by ZAP-70 and CD38 could be used in guiding treatment decisions and they probably should be recommended to all patients with B-CLL in trying to obtain a more clear profile of the disease at the time of diagnosis.
The relationship between micronutrients and anthropometric measurements in malnourished children / Relaţia între micronutrienţi şi măsurătorile antropometrice la copiii malnutriţi
Authors: Man Lidia , Man Adrian , Mărginean Cristina Oana , Pitea Ana Maria , Baghiu Maria Despina 
Published: January 2015

AbstractPurpose: to evaluate the serum levels of micronutrients in children with nutritional disorders, and to find if there is a direct correlation between them and the anthropometric measurements. Materials and methods: the study was conducted on 125 children (0-18 years); the working group consisted in children with Z-score < -2 standard deviations for at least one anthropometric measurement, while the children without growth disorders were considered as controls. Thus, for each anthropometric measurement, we had different working/control groups that were used for the assessment of correlation with laboratory findings. We followed eight anthropometric parameters and their relation with five of the micronutrients (Ca, Mg, Fe, Zn and Cu). Results: no statistical differences were found in micronutrients serum levels between genders or provenance. Most mean serum levels of micronutrients were lower in the children with Z-scores < -2 standard deviations (except Cu). Mg and Ca were positively correlated with most of the anthropometric measurements. For Fe, Zn and Cu, we found no correlation with any of the anthropometric measures. Differences in mean serum levels were found for Mg, with lower values in children with low weight-forage and triceps-skinfold-thickness, and for Cu, with higher levels in children with low triceps-skinfold-thickness. The red blood cell indices were positively associated with Fe and Zn levels. Conclusions: correlations between the serum level of micronutrients and anthropometric evaluation scores were found for Mg and Ca, but not for Fe and Zn, which were instead directly correlated with red blood cells indices. Mg, Fe and Zn tend to present small serum values in children with growth deficits. Considering the costs, the routine evaluation of Zn and Cu serum levels in growth disorder suspicion is not justified in our geographic area.
Atypical case of B-cell Chronic Lymphocytic Leukemia presenting with extreme hyperleukocytosis / Hiperleucocitoză extremă într-un caz atipic de leucemie limfatică cronică cu celulă B
Authors: Bacârea Anca , Bănescu Claudia , Macarie Ioan , Köpeczi Judit Beáta , Dorcioman Bogdana 
Published: January 2015

AbstractVery few cases of chronic lymphocytic leukemia (CLL) presenting with extreme hyperleukocytosis are reported in the literature. We describe the case of a 66 years old woman, with newly diagnosed CLL presenting with extreme hyperleukocytosis of 774.2 x 109/liter, Rai stage III and Binet stage C. The patient has no comorbidities and the CIRS score (cumulative illness rating scale) is well below 6, with normal creatinine clearance. Some other interesting aspects related with this case are the atypical immunophenotype, the expression of Cyclin D1, and the B hepatitis viral infection, which made her diagnosis and treatment challenging. The patient was tested for NOTCH1 mutation and it was positive. There is important evidence that NOTCH1 mutations are associated with rapidly progressive disease and resistance to treatment. The distinction of CLL from mantle cell lymphoma (MCL) is not always easy because some MCLs may mimic CLL clinically, histologically, and/or phenotypically. The hepatitis B prophylaxis for viral reactivation was not available an in the end the patient was treated only with fludarabine and cyclophosphamide, without rituximab. CD200 should be introduced in the routine panel for flow cytometry to distinguish CLL from mantle cell lymphoma and NOTCH1 mutation is associated with poor prognosis and should be evaluated at diagnosis. CLL with extreme hyperleukocytosis presentation is very rare and sometimes an atypical CLL may represent a diagnostic pitfall.
Egalitarian Awareness in the Capitalistic Reality. The Entanglement of Poverty and Wealth in Daily Social Sphere 25 Years after the System Change in Poland
Authors: Żuk Piotr 
Published: January 2015

Abstract
The purpose of the article is to illustrate the social order preferred by the Polish together with their assessment of the existing social inequalities. On the basis of the empirical material collected among the sample of 1000 respondents, the findings show manners of their definition of equity, perception of discrepancies between the ideal of equity and the existing real status, together with their assessment of the existing differences in the treatment of rich and poor people by various institutions (banks, police, hospitals, courts). The article also describes the causes of social inequalities as indicated by the respondents and their assessment of state policies in reference to them. The article concludes that 25 years after the collapse of “real socialism”, Polish society presents a firmly egalitarian awareness that is in conflict with the current market order.
Diagnosis-Related Groups (DRG) and Hospital Business Performance Management
Authors: Szynkiewicz Piotr , Iltchev Petre , Piechota Anna , Sierocka Aleksandra , Marczak Michał 
Published: January 2015

Abstract
The goal of this article is to present the possibility of using Diagnosis- Related Groups (DRG) in the hospital management process and to analyse the need for business performance management on the part of hospital management staff. The following research methods were used: literature analysis, case studies, and poll analysis. It is not possible to increase the effectiveness of operation of healthcare entities without increasing the importance of IT systems and using DRG more effectively in the management process. Training users in IT and the use of DRGs is important to achieving hospital effectiveness. The increased importance of analyses and planning in a hospital should be reflected in the organisational structure of service providers. Hospital controllers should have a similar role to those present in most companies in other industries.
The saker falcon (Falco cherrug) population, diet and nest boxes in Slovakia: LIFE-project report 2011–2014
Authors: Chavko Jozef , Slobodník Roman , Deutschová Lucia , Lipták Ján , Mihók Jozef , Obuch Ján , Nemcek Vladimír 
Published: January 2015

Abstract
From October 201 0 until December 201 4 the LIFE09 NAT/HU/000384 project was implemented in four Special Protected Areas in Slovakia. The recent project was complementary to a previous LIFE project implemented between 2006 and 201 0. Both projects supported regular monitoring of the population of saker falcon and new approaches to conservation of the species. The LIFE09 project helped to increase knowledge about the prey composition and the behaviour of the species. The population was strongly affected in 201 3 by extremely bad weather conditions (1 .79 fledglings/breeding attempt). Although the total number of saker falcon pairs in Slovakia is thought to be higher, 31 pairs were breeding in 201 4. By analysis of the diet (2991 individuals), 47 prey species were identified, from which birds were predominant (80%). More than half of the diet consisted of feral pigeons (Columba livia f. domestica) (59%)
Movement of satellite-tracked juvenile saker falcons (Falco cherrug) in SW Slovakia
Authors: Nemcek Vladimír , Chavko Jozef , Deutschová Lucia 
Published: January 2015

Abstract
During the period 2008-2011 nine juvenile saker falcon females from SW Slovakia were tracked with satellite telemetry. The movement of four of those females tagged with satellite tracking devices was analysed in 2011 (two juveniles for 6 months, one for 5 months and one for 3 months). The analysis of the collision risk with wind turbines also used telemetry data from previous years (2008-2009) - five birds tracked from abandoning their nest to abandoning their post-fledging areas (i.e. 45, 48, 37, 11 3 and 1 34 days). The juveniles visited 1 3 different countries during their post-fledging dispersal and migration. Maximum distance from the nest varied between 1 53 and 2094 km. For two birds the cause of death was recorded: one female was electrocuted and other female was probably also electrocuted. We also evaluated two factors of potential wind turbine threat: a) the distance ofGPS locations from the nearest wind turbines in the vicinity of post-fledging areas, b) numbers ofwind turbines in post-fledging areas. Assessment of the collision risk with wind turbines showed low risk for the tracked individuals. Two of four tracked birds were at medium risk (2-5 km) from wind turbines. The highest risk for the young falcons comes from wind turbines in neighbouring countries, where they are constructed near borders
Winter ranging behaviour of a greater spotted eagle (Aquila clanga) in southeast Spain during four consecutive years
Authors: Pérez-García Juan M. , Sellis Urmas , Väli Ülo 
Published: January 2015

Abstract
Knowing the winter behaviour is essential for deciding on conservation strategies for threatened migratory species such as the greater spotted eagle (Aquila clanga). Fidelity and inter-annual variation in winter home range of an Estonian greater spotted eagle were studied during the first four years of its life by means ofGPS satellite telemetry in south-eastern Spain. Results show the eagle exploited a small area (1 2.7 km2, 95% kernel) with high inter-annual fidelity during all winter stages. The A. clanga preferred marshes and water bodies and avoided irrigated crops and urban areas. Waterfowl hunting did not show any effect on the spatial pattern of the eagle’s behaviour, although water level management in reservoirs could influence their use by the A. clanga. Our study highlights that the wintering home range may be limited to a small suitable habitat patch where human activities, especially water reservoir management, should be regulated
Can in Shakespeare and Marlowe
Authors: Skorasińska Monika 
Published: January 2015

ABSTRACT
This paper seeks to present the main meanings and the use of the modal verb can in the plays of two Early Modern English playwrights, William Shakespeare and Christopher Marlowe. In particular, the study aims at presenting a comparative analysis and provides descriptive as well as quantitative data. The research is based on the analysis of the corpus consisting of the plays written by Shakespeare and Marlowe between 1593-1599. The choice of the works is not random but includes the plays which bear the strongest resemblance in terms of theme, structure, and most importantly, the language of both authors.
Prewintering Aquatic and Semiaquatic True Bugs in the Timiş River Valley: Adaptations, Diversity, Community Structure and the Role of Antropic Impact
Authors: Olosutean Horea , Ilie Daniela 
Published: January 2015

ABSTRACT
In November 2011 during a normal overwintering period, a small number of active, aquatic and semiaquatic adult insects were collected from several habitats along the Timiș River basin. This active state for the insects was closely related to higher temperatures found in certain deeper, stagnant water habitats, which also had greater amounts of aquatic vegetation and more stable living conditions. Most such habitats seem to be of anthropic origin, emphasizing the role of human impacts on the life of the discussed group of insects and the need to consider anthropic activities in the conservation of certain species of insects. The state of Aphelocheirus aestivalis is also discussed, along with a proposal to study the species on a larger scale and its possible inclusion on the Romanian Red List.
Timiş River (Banat, Romania) Benthic Macroinvertebrate Communities Structure Spatial Dinamic
Authors: Curtean-Bănăduc Angela , Farcaş Adina Nicoleta 
Published: January 2015

ABSTRACT
This study presents the description of the structure of benthic macroinvertebrate communities of the Timiș River (Danube Watershed) in correlation with environmental parameters. The results are based on quantitative benthic macroinvertebrates (105 samples) taken in 2011 (June-September) from 21 stations of the the Timiş River, situate between its sources and the Romanian-Serbian border (241 km). The assessed biotope variables were: altitude, slope, riverbed width, depth, substratum types, channel modification and water physico-chemical caracteristics. The results of the study reveal that the spatial structure of benthic macroinvertebrates is induced by the substrate type, by the minor riverbed modifications in comparison with the natural conditions and the quantities of oxidable matters in the water.
The Diversity of Caddisflies (Insecta, Trichoptera) Species in TimiȘ River Catchment Area (Western Romania)
Authors: Pârvu Mălina , Pârvulescu Lucian 
Published: January 2015

ABSTRACT
In order to determine the benthic macro invertebrates communities role as bioindicators, researchers worldwide carried out analyses into the structure, dynamics and diversity of the different groups as well as into the physical-chemical factors. A total of twenty one species of caddisfly larvae were identified in the study. Numerical abundance, frequency and diversity values recorded for the caddisfly species varied according to the physicalchemical conditions specific to each sample collecting station. The physical and chemical parameters monitored in Timiș River water catchment basin have corresponded with the limits of the Ministry of Environment and Water Management (MEWA) Order 161/2006, which states the ecological status of surface bodies of water with, few exceptions being identified.
Alkaline Lake System in Danube and Tisza Interfluve (Szeged, Hu) – Climate Change and Landscape Degradation
Authors: Halmos László , Bozsó Gábor , Pál-Molnár Elemér 
Published: January 2015

Abstract
Sodic areas can be found in every continent. The main condition of the salt accumulation in sodic soils is the near-surface groundwater. There is a serious environmental problem in the Danube-Tisza Interfluve which threatens the natural alkaline lakes and sodic areas with landscape destruction. The aim of this study is to collect information about the seasonal geochemical cycles in the sediments of Lake Fehér. Based on the results of this research, the protection of the similar alkaline wetlands could be more effective. The study area was an alkaline lake system sorrounded by sodic areas called Lake Fehér by Szeged. The investigation was carried out in three periods based on the seasonal distribution of precipitation. There were eight sampling dates on the same site during one meteorological year. For the short-term monitoring, the type of the soil, the particle content, the pH and the EC were investigated. The groundwater and precipitation data of the last century were used for the long-term observation. The results show that the precipitation decreased in the southern part of the Danube-Tisza Interfluve in the last 100 years. Along with the decreasing precipitation, the groundwater level has been sinking. Knowing the dynamics of the area, the problem could be managed; otherwise, the ecosystems of the alkaline lakes will vanish.