bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emotion Socialization in the Context of Risk and Psychopathology: Maternal Emotion Coaching Predicts Better Treatment Outcomes for Emotionally Labile Children with Oppositional Defiant Disorder
Authors: Dunsmore Julie C. , Booker Jordan A. , Ollendick Thomas H. , Greene Ross W. 
Published: February 2015

AbstractWe examined whether maternal emotion coaching at pretreatment predicted children's treatment response following a 12‐week program addressing children's oppositional defiant disorder (ODD) symptoms. A total of 89 mother–child dyads participated. At pretreatment, mothers and children engaged in an emotion talk task. Mothers also reported their beliefs about emotions at pretreatment and their child's disruptive behavior symptoms, emotion regulation, and emotion lability/negativity at pre‐, mid‐, and post‐treatment. Clinicians reported children's symptom severity at pre‐ and post‐treatment. Children's emotion lability/negativity moderated effects of maternal emotion coaching on children's post‐treatment ODD symptoms, with stronger benefits of emotion coaching for children high in emotion lability/negativity. Results suggest that emotion coaching may promote treatment response for children with ODD who are especially at risk due to their emotionality.
Exploring the Bidirectionality of Emotion Understanding and Classroom Behavior with Spanish‐ and English‐speaking Preschoolers Attending Head Start
Authors: Strand Paul S. , Barbosa‐Leiker Celestina , Arellano Piedra Maria , Downs Andrew 
Published: February 2015

AbstractThe present study investigated time‐dependent relationships between emotion understanding and the behavioral adjustment of preschoolers over a single school year using a latent variable structural equation modeling framework. Teacher reports of child behavior (hyperactivity, emotion symptoms, conduct problems, peer problems, and prosocial behavior) and performance assessments of emotion understanding were obtained twice at a 6‐month interval for a sample of 281 preschoolers (159 boys and 122 girls, with mean age = 52.40 months) from English‐ (N = 158) and Spanish‐speaking (N = 123) backgrounds. Emotion understanding and behavior were stable over time, and cross‐sectional associations between them were in expected directions. Cross‐lagged paths revealed that the behavior variables significantly associated with emotion understanding across time were hyperactivity, emotion symptoms, and peer problems, and that behavior variables were generally better predictors of emotion understanding than vice versa. Differences across gender and language groups suggest a stronger and more complex bidirectional relationship between emotion understanding and behavior for girls and for Spanish‐speaking children compared wth boys and English‐speaking children. Results are discussed with respect to the value of exploring cross‐lagged relationships and the potential importance of gender and culture as determinants of those relationships.
Dimensions of Parenting Associated with Child Prekindergarten Emotion Regulation and Attention Control in Low‐income Families
Authors: Mathis Erin T. B. , Bierman Karen L. 
Published: February 2015

AbstractDelays in emotion regulation and attention control are common among children growing up in poverty, and they contribute to significant socioeconomic gaps in school readiness and later school attainment. In this study, the emotion regulation and attention control skills of 210 prekindergarten Head Start participants were assessed (M age = 4.80 years old). Home interviews and videotaped parent–child interactions were used to evaluate three aspects of parenting (e.g., warm‐sensitive, directive‐critical, and parenting stress). Structural equation models documented significant, unique associations linking directive‐critical parenting and parenting stress with poor child emotion regulation skills. Directive‐critical parenting was also uniquely associated with low levels of child attention control. Warm‐sensitive parenting was not uniquely related to either emotion regulation or attention control at this age. The findings suggest that, by prekindergarten, parent stress management and reduced directiveness emerge as the primary correlates of child emotion regulation and attention control whereas warm‐sensitive parenting plays a diminished role.
EQOFIX: a combined economic and quality‐of‐life study of hemophilia B treatments in France
Authors: Polack Benoît , Calvez Thierry , Chambost Hervé , Rothschild Chantal , Goudemand Jenny , Claeyssens Ségolène , Borel‐Derlon Annie , Bardoulat Isabelle , Maurel Frédérique , Woronoff‐Lemsi Marie‐Christine ,   for the EQOFIX Study Group
Published: February 2015

BACKGROUNDEQOFIX is a medicoeconomic study that analyzed the health‐related quality of life (HRQoL) and costs of care of the moderate and severe forms of hemophilia B, treated on demand or by prophylaxis with either plasma‐derived Factor IX (pdFIX) or recombinant FIX (rFIX).STUDY DESIGN AND METHODSThe primary objectives were evaluations of the impact of hemophilia B on HRQoL and of the costs associated with its management. The secondary objectives were evaluations of the clinical efficacy and costs of care of pdFIX and rFIX. In this observational study we included and followed for 1 year severe and moderate hemophilia B patients without inhibitor. HRQoL was evaluated through generic and disease‐specific questionnaires. Information on the health resources consumed was collected every 3 months.RESULTSThe EQOFIX cohort was composed of 155 patients, including 51 children and 104 adults, with 114 having severe disease and 41 having moderate disease. The regimens were prophylactic for 61 and on demand for 94. Altogether, 78 were treated with rFIX and 77 with pdFIX. There was no difference in the QoL between the pdFIX and rFIX treatments. The extra cost of prophylaxis was €22,605 per bleeding event prevented. The consumption of FIX was 1.4‐fold higher for the patients treated with rFIX than for the patients treated with pdFIX.CONCLUSIONOur findings in a cohort composed of 25% of the French population of moderate and severe hemophilia B patients show, with similar clinical and HRQoL results, that treatment with rFIX is more expensive than treatment with pdFIX.
Evaluation of Osteogenic Potentials of Avian Demineralized Bone Matrix in the Healing of Osseous Defects in Pigeons
Authors: Sanaei Reza , Abu Jalila , Nazari Mojgan , Zuki Mohd A. B. , Allaudin Zeenathul N. 
Published: February 2015

ObjectivesTo evaluate avian allogeneic demineralized bone matrix (DBM) in the healing of long bone defects as a function of geometry and time in a pigeon model.Study DesignExperimental.AnimalsAdult rock pigeons (n = 60).MethodsMidshaft ulnar osseous defects were grafted with 2 geometric forms of DBM (tubular vs. chipped) and stabilized with a hybrid fixator. Autologous chips of sternal keel were used in a third group as control. Outcomes were evaluated by radiography and histology/histomorphometry at 4, 8, 12, and 24 weeks postoperatively.ResultsDespite an early rapid healing response, autografts plateaued (histologic score and new bone area) by 8 weeks with no significant improvement afterwards. Conversely, allogeneic DBM implants demonstrated continuous temporal improvement in bone healing, and tubular DBM finally outpaced autograft implants after week 12 with values for metrics achieving statistical significance by week 24. Chip DBM was inferior to tubular DBM and autograft.ConclusionsAvian DBM is osteogenic, biocompatible, and safe in orthotopic sites with potential usefulness in avian bone grafting. Implant geometry (shape and size) affects such osteogenic potentials.
The KCNQ1 channel – remarkable flexibility in gating allows for functional versatility
Authors: Liin Sara I. , Barro‐Soria Rene , Larsson H. Peter 
Published: February 2015

AbstractThe KCNQ1 channel (also called Kv7.1 or KvLQT1) belongs to the superfamily of voltage‐gated K+ (Kv) channels. KCNQ1 shares several general features with other Kv channels but also displays a fascinating flexibility in terms of the mechanism of channel gating, which allows KCNQ1 to play different physiological roles in different tissues. This flexibility allows KCNQ1 channels to function as voltage‐independent channels in epithelial tissues, whereas KCNQ1 function as voltage‐activated channels with very slow kinetics in cardiac tissues. This flexibility is in part provided by the association of KCNQ1 with different accessory KCNE β‐subunits and different modulators, but also seems like an integral part of KCNQ1 itself. The aim of this review is to describe the main mechanisms underlying KCNQ1 flexibility.
Cholesterol modulates open probability and desensitization of NMDA receptors
Authors: Korinek Miloslav , Vyklicky Vojtech , Borovska Jirina , Lichnerova Katarina , Kaniakova Martina , Krausova Barbora , Krusek Jan , Balik Ales , Smejkalova Tereza , Horak Martin , Vyklicky Ladislav 
Published: February 2015

Key points
NMDA receptors (NMDARs) are tetrameric cation channels permeable to calcium; they mediate excitatory synaptic transmission in the CNS and their excessive activation can lead to neurodegeneration.
Although these receptors are in direct contact with plasma membrane, lipid–NMDAR interactions are little understood.
Using cultured rat cerebellar granule cells, we show that acute and chronic pretreatments resulting in cell cholesterol depletion profoundly diminish NMDAR responses and increase NMDAR desensitization, and also that cholesterol enrichment potentiates NMDAR responses; however, cholesterol manipulation has no effect on the amplitude of AMPA/kainate receptor responses.
Diminution of NMDAR responses by cholesterol depletion is the result of a reduction of the ion channel open probability, whereas the increase in receptor desensitization is the result of an increase in the rate constant of entry into the desensitized state.
These results demonstrate the physiological role of membrane lipids in the modulation of NMDAR activity.
AbstractNMDA receptors (NMDARs) are glutamate‐gated ion channels that mediate excitatory neurotransmission in the CNS. Although these receptors are in direct contact with plasma membrane, lipid–NMDAR interactions are little understood. In the present study, we aimed at characterizing the effect of cholesterol on the ionotropic glutamate receptors. Whole‐cell current responses induced by fast application of NMDA in cultured rat cerebellar granule cells (CGCs) were almost abolished (reduced to 3%) and the relative degree of receptor desensitization was increased (by seven‐fold) after acute cholesterol depletion by methyl‐β‐cyclodextrin. Both of these effects were fully reversible by cholesterol repletion. By contrast, the responses mediated by AMPA/kainate receptors were not affected by cholesterol depletion. Similar results were obtained in CGCs after chronic inhibition of cholesterol biosynthesis by simvastatin and acute enzymatic cholesterol degradation to 4‐cholesten‐3‐one by cholesterol oxidase. Fluorescence anisotropy measurements showed that membrane fluidity increased after methyl‐β‐cyclodextrin pretreatment. However, no change in fluidity was observed after cholesterol enzymatic degradation, suggesting that the effect of cholesterol on NMDARs is not mediated by changes in membrane fluidity. Our data show that diminution of NMDAR responses by cholesterol depletion is the result of a reduction of the open probability, whereas the increase in receptor desensitization is the result of an increase in the rate constant of entry into the desensitized state. Surface NMDAR population, agonist affinity, single‐channel conductance and open time were not altered in cholesterol‐depleted CGCs. The results of our experiments show that cholesterol is a strong endogenous modulator of NMDARs.
Local patient dose diagnostic reference levels in pediatric interventional cardiology in Chile using age bands and patient weight values
Authors: Ubeda Carlos , Miranda Patricia , Vano Eliseo 
Published: February 2015

Purpose:To present the results of a patient dose evaluation program in pediatric cardiology and propose local diagnostic reference levels (DRLs) for different types of procedure and age range, in addition to suggesting approaches to correlate patient dose values with patient weight. This study was the first conducted in Latin America for pediatric interventional cardiology under the auspices of the International Atomic Energy Agency.Methods:Over three years, the following data regarding demographic and patient dose values were collected: age, gender, weight, height, number of cine series, total number of cine frames, fluoroscopy time (FT), and two dosimetric quantities, dose‐area product (DAP) and cumulative dose (CD), at the patient entrance reference point. The third quartile values for FT, DAP, CD, number of cine series, and the DAP/body weight ratio were proposed as the set of quantities to use as local DRLs.Results:Five hundred and seventeen patients were divided into four age groups. Sample sizes by age group were 120 for <1 yr; 213 for 1 to <5 yr; 82 for 5 to <10 yr; and 102 for 10 to <16 yr. The third quartile values obtained for DAP by diagnostic and therapeutic procedures and age range were 1.17 and 1.11 Gy cm2 for <1 yr; 1.74 and 1.90 Gy cm2 for 1 to <5 yr; 2.83 and 3.22 Gy cm2 for 5 to <10 yr; and 7.34 and 8.68 Gy cm2 for 10 to <16 yr, respectively. The third quartile value obtained for the DAP/body weight ratio for the full sample of procedures was 0.17 (Gy cm2/kg) for diagnostic and therapeutic procedures.Conclusions:The data presented in this paper are an initial attempt at establishing local DRLs in pediatric interventional cardiology, from a large sample of procedures for the standard age bands used in Europe, complemented with the values of the ratio between DAP and patient weight. This permits a rough estimate of DRLs for different patient weights and the refining of these values for the age bands when there may be large differences in child size. These DRLs were obtained at the largest pediatric hospital in Chile, with an active optimization program, and could be used by other hospitals in the Latin America region to compare their current patient dose values and determine whether corrective action is appropriate.
Computer aided wireless capsule endoscopy video segmentation
Authors: Li Baopu , Xu Guoqing , Zhou Ran , Wang Tianfu 
Published: February 2015

Purpose:Wireless capsule endoscopy (WCE) opens a new door for the digestive tract examination and diagnosis. However, the examination of its video data is tedious. This study aims to assist a physician to interpret a WCE video by segmenting it into different anatomic parts in the digestive tract.Methods:A two level WCE video segmentation scheme is proposed to locate the boundary between the stomach, small intestine, and large intestine. In the rough level, the authors utilize color feature to draw a dissimilarity curve for a WCE video and obtain an approximate boundary. Meanwhile, training data for the fine level segmentation can be collected automatically between the two approximate boundaries of organs to overcome the difficulty of training data collection in traditional approaches. In the fine level, color histogram in the HSI color space is used to segment the stomach and small intestine. Then, color uniform local binary pattern (CULBP) algorithm is applied for discrimination of the small intestine and large intestine, which includes two patterns, namely, color norm and color angle pattern. The CULBP feature is robust to variation of illumination and discriminative for classification. In order to increase the performance of support vector machine, the authors integrate it with the Adaboost approach. Finally, the authors refine the classification results to segment a WCE video into different parts, that is, the stomach, small intestine, and large intestine.Results:The average precision and recall are 91.2% and 90.6% for the stomach/small intestine classification, 89.2% and 88.7% for the small/large intestine discrimination. Paired t‐test also demonstrates a significant better performance of the proposed scheme compared to some traditional methods. The average segmentation error is 8 frames for the stomach/small intestine discrimination, and 14 frames for the small/large intestine segmentation.Conclusions:The results have demonstrated that the new video segmentation method can accurately locate the boundary between different organ regions in a WCE video. Such a segmentation result may enhance the efficiency of WCE examination.
Programming colloidal phase transitions with DNA strand displacement
Authors: Rogers W. Benjamin , Manoharan Vinothan N. 
Published: February 2015

DNA control of bonding interactions
Colloidal particles have been used as atom mimics and are often connected together using complementary DNA strands. Rogers and Manoharan controlled the strength of the colloidal “bond” by using a set of competing strand displacement reactions. They capitalized on the reversible chemical equilibrium between the DNA strands connecting different particles to control the temperature dependence of the equilibrium state.

Science
, this issue p.
639
Efficacy of Human Papillomavirus 16 and 18 (HPV-16/18) AS04-Adjuvanted Vaccine against Cervical Infection and Precancer in Young Women: Final Event-Driven Analysis of the Randomized, Double-Blind PATRICIA Trial
Authors: Apter Dan , Wheeler Cosette M. , Paavonen Jorma , Castellsagué Xavier , Garland Suzanne M. , Skinner S. Rachel , Naud Paulo , Salmerón Jorge , Chow Song-Nan , Kitchener Henry C. , Teixeira Julio C. , Jaisamrarn Unnop , Limson Genara , Szarewski Anne , Romanowski Barbara , Aoki Fred Y. , Schwarz Tino F. , Poppe Willy A. J. , Bosch F. Xavier , Mindel Adrian , de Sutter Philippe , Hardt Karin , Zahaf Toufik , Descamps Dominique , Struyf Frank , Lehtinen Matti , Dubin Gary 
Published: February 2015

ABSTRACT

We report final event-driven analysis data on the immunogenicity and efficacy of the human papillomavirus 16 and 18 ((HPV-16/18) AS04-adjuvanted vaccine in young women aged 15 to 25 years from the PApilloma TRIal against Cancer In young Adults (PATRICIA). The total vaccinated cohort (TVC) included all randomized participants who received at least one vaccine dose (vaccine,
n
= 9,319; control,
n
= 9,325) at months 0, 1, and/or 6. The TVC-naive (vaccine,
n
= 5,822; control,
n
= 5,819) had no evidence of high-risk HPV infection at baseline, approximating adolescent girls targeted by most HPV vaccination programs. Mean follow-up was approximately 39 months after the first vaccine dose in each cohort. At baseline, 26% of women in the TVC had evidence of past and/or current HPV-16/18 infection. HPV-16 and HPV-18 antibody titers postvaccination tended to be higher among 15- to 17-year-olds than among 18- to 25-year-olds. In the TVC, vaccine efficacy (VE) against cervical intraepithelial neoplasia grade 1 or greater (CIN1+), CIN2+, and CIN3+ associated with HPV-16/18 was 55.5% (96.1% confidence interval [CI], 43.2, 65.3), 52.8% (37.5, 64.7), and 33.6% (−1.1, 56.9). VE against CIN1+, CIN2+, and CIN3+ irrespective of HPV DNA was 21.7% (10.7, 31.4), 30.4% (16.4, 42.1), and 33.4% (9.1, 51.5) and was consistently significant only in 15- to 17-year-old women (27.4% [10.8, 40.9], 41.8% [22.3, 56.7], and 55.8% [19.2, 76.9]). In the TVC-naive, VE against CIN1+, CIN2+, and CIN3+ associated with HPV-16/18 was 96.5% (89.0, 99.4), 98.4% (90.4, 100), and 100% (64.7, 100), and irrespective of HPV DNA it was 50.1% (35.9, 61.4), 70.2% (54.7, 80.9), and 87.0% (54.9, 97.7). VE against 12-month persistent infection with HPV-16/18 was 89.9% (84.0, 94.0), and that against HPV-31/33/45/51 was 49.0% (34.7, 60.3). In conclusion, vaccinating adolescents before sexual debut has a substantial impact on the overall incidence of high-grade cervical abnormalities, and catch-up vaccination up to 18 years of age is most likely effective. (This study has been registered at
ClinicalTrials.gov
under registration no. NCT001226810.)
Development of a Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against the 3B Protein of Foot-and-Mouth Disease Virus
Authors: Yang Ming , Parida Satya , Salo Tim , Hole Kate , Velazquez-Salinas Lauro , Clavijo Alfonso 
Published: February 2015

ABSTRACTFoot-and-mouth disease (FMD) is one of the most highly contagious and economically devastating diseases, and it severely constrains the international trade of animals. Vaccination against FMD is a key element in the control of FMD. However, vaccination of susceptible animals raises critical issues, such as the differentiation of infected animals from vaccinated animals. The current study developed a reliable and rapid test to detect antibodies against the conserved, nonstructural proteins (NSPs) of the FMD virus (FMDV) to distinguish infected animals from vaccinated animals. A monoclonal antibody (MAb) against the FMDV NSP 3B was produced. A competitive enzyme-linked immunosorbent assay (cELISA) for FMDV/NSP antibody detection was developed using a recombinant 3ABC protein as the antigen and the 3B-specific MAb. Sera collected from naive, FMDV experimentally infected, vaccinated carrier, and noncarrier animals were tested using the 3B cELISA. The diagnostic specificity was 99.4% for naive animals (cattle, pigs, and sheep) and 99.7% for vaccinated noncarrier animals. The diagnostic sensitivity was 100% for experimentally inoculated animals and 64% for vaccinated carrier animals. The performance of this 3B cELISA was compared to that of four commercial ELISA kits using a panel of serum samples established by the World Reference Laboratory for FMD at The Pirbright Institute, Pirbright, United Kingdom. The diagnostic sensitivity of the 3B cELISA for the panel of FMDV/NSP-positive bovine serum samples was 94%, which was comparable to or better than that of the commercially available NSP antibody detection kits. This 3B cELISA is a simple, reliable test to detect antibodies against FMDV nonstructural proteins.
Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Identifies Pseudomonas aeruginosa High-Risk Clones
Authors: Cabrolier Nadège , Sauget Marlène , Bertrand Xavier , Hocquet Didier 
Published: February 2015

ABSTRACT

We show here that matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) accurately and quickly identified the five high-risk clones of
Pseudomonas aeruginosa
sequence type 111 (ST111), ST175, ST235, ST253, and ST395. The use of this screening technique by clinical microbiology laboratories may tackle the spread of high-risk clones by the quick implementation of hygiene control procedures for relevant patients.
Comparison of MGIT and Myco/F Lytic Liquid-Based Blood Culture Systems for Recovery of Mycobacterium tuberculosis from Pleural Fluid
Authors: Harausz Elizabeth , Lusiba John Kafuluma , Nsereko Mary , Johnson John L. , Toossi Zahra , Ogwang Sam , Boom W. Henry , Joloba Moses L. 
Published: February 2015

ABSTRACT

The specificities and sensitivities of the Bactec mycobacterial growth indicator tube (MGIT) system for the recovery of
Mycobacterium tuberculosis
from pleural fluid are not statistically different than those of the Myco/F lytic liquid culture system. The time to positivity is shorter in the MGIT system (12.7 versus 20.7 days, respectively;
P
= 0.007). The Myco/F lytic culture system may be an alternative to the MGIT system for diagnosing pleural tuberculosis.