bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydrogen Peroxide as a Damage Signal in Tissue Injury and Inflammation: Murderer, Mediator, or Messenger?
Authors: van der Vliet Albert , Janssen‐Heininger Yvonne M.W. 
Published: October 2013

ABSTRACTTissue injury and inflammation are associated with increased production of reactive oxygen species (ROS), which have the ability to induce oxidative injury to various biomolecules resulting in protein dysfunction, genetic instability, or cell death. However, recent observations indicate that formation of hydrogen peroxide (H2O2) during tissue injury is also an essential feature of the ensuing wound healing response, and functions as an early damage signal to control several critical aspects of the wound healing process. Because innate oxidative wound responses must be tightly coordinated to avoid chronic inflammation or tissue injury, a more complete understanding is needed regarding the origins and dynamics of ROS production, and their critical biological targets. This prospect highlights the current experimental evidence implicating H2O2 in early epithelial wound responses, and summarizes technical advances and approaches that may help distinguish its beneficial actions from its more deleterious actions in conditions of chronic tissue injury or inflammation. J. Cell. Biochem. 115: 427–435, 2014. © 2013 Wiley Periodicals, Inc.
ErbB2 Activation Upregulates Glutaminase 1 Expression Which Promotes Breast Cancer Cell Proliferation
Authors: Qie Shuo , Chu Clarissa , Li Weihua , Wang Chenguang , Sang Nianli 
Published: October 2013

AbstractActive glutamine utilization is critical for tumor cell proliferation. Glutaminolysis represents the first and rate‐limiting step of glutamine utilization and is catalyzed by glutaminase (GLS). Activation of ErbB2 is one of the major causes of breast cancers, the second most common cause of death for women in many countries. However, it remains unclear whether ErbB2 signaling affects glutaminase expression in breast cancer cells. In this study, we show that MCF10A‐NeuT cell line has higher GLS1 expression at both mRNA and protein levels than its parental line MCF10A, and knockdown of ErbB2 decreases GLS1 expression in MCF10A‐NeuT cells. We further show that in these cells, ErbB2‐mediated upregulation of GLS1 is not correlated to c‐Myc expression. Moreover, activation of neither PI3K‐Akt nor MAPK pathway is sufficient to upregulate GLS1 expression. Interestingly, inhibition of NF‐κB blocks ErbB2‐stimulated GLS1 expression, whereas stimulation of NF‐κB is sufficient to enhance GLS1 levels in MCF10A cells, suggesting a PI3K‐Akt‐independent activation of NF‐κB upregulates GLS1 in ErbB2‐positive breast cancer cells. Finally, knockdown or inhibition of GLS1 significantly decreased the proliferation of breast cancer cells with high GLS1 levels. Taken together, our data indicate that ErbB2 activation promotes GLS1 expression via a PI3K‐Akt‐independent NF‐κB pathway in breast cancer cells, identifying another oncogenic signaling pathway which stimulates GLS1 expression, and thus promoting glutamine utilization in cancer cells. These findings, if validated by in vivo model, may facilitate the identification of novel biochemical targets for cancer prevention and therapy. J. Cell. Biochem. 115: 498–509, 2014. © 2013 Wiley Periodicals, Inc.
Dab2, Megalin, Cubilin and Amnionless Receptor Complex Might Mediate Intestinal Endocytosis in the Suckling Rat
Authors: Vázquez‐Carretero María D. , Palomo Marta , García‐Miranda Pablo , Sánchez‐Aguayo Inmaculada , Peral María J. , Calonge María L. , Ilundain Anunciación A. 
Published: October 2013

ABSTRACTWe previously proposed that Dab2 participates in the endocytosis of milk macromolecules in rat small intestine. Here we investigate the receptors that may mediate this endocytosis by studying the effects of age and diet on megalin, VLDLR, and ApoER2 expression, and that of age on the expression of cubilin and amnionless. Of megalin, VLDLR and ApoER2, only the megalin expression pattern resembles that of Dab2 previously reported. Thus the mRNA and protein levels of megalin and Dab2 are high in the intestine of the suckling rat, down‐regulated by age and up‐regulated by milk diet, mainly in the ileum. Neither age nor diet affect ApoER2 mRNA levels. The effect of age on VLDLR mRNA levels depends on the epithelial cell tested but they are down‐regulated by milk diet. In the suckling rat, the intestinal expressions of both cubilin and amnionless are similar to that of megalin and megalin, cubilin, amnionless and Dab2 co‐localize at the microvilli and in the apical endocytic apparatus. Co‐localization of Dab2 with ApoER2 and VLDLR at the microvilli and in the apical endocytic apparatus is also observed. This is the first report showing intestinal co‐localization of: megalin/cubilin/amnionless/Dab2, VLDLR/Dab2 and ApoER2/Dab2. We conclude that the megalin/cubilin/amnionless/Dab2 complex/es participate in intestinal processes, mainly during the lactation period and that Dab2 may act as an adaptor in intestinal processes mediated by ApoER2 and VLDLR. J. Cell. Biochem. 115: 510–522, 2014. © 2013 Wiley Periodicals, Inc.
Nanotopography Drives Stem Cell Fate Toward Osteoblast Differentiation Through α1β1 Integrin Signaling Pathway
Authors: Rosa A.L. , Kato R.B. , Castro Raucci L.M.S. , Teixeira L.N. , de Oliveira F.S. , Bellesini L.S. , de Oliveira P.T. , Hassan M.Q. , Beloti M.M. 
Published: October 2013

ABSTRACTThe aim of our study was to investigate the osteoinductive potential of a titanium (Ti) surface with nanotopography, using mesenchymal stem cells (MSCs) and the mechanism involved in this phenomenon. Polished Ti discs were chemically treated with H2SO4/H2O2 to yield nanotopography and rat MSCs were cultured under osteogenic and non‐osteogenic conditions on both nanotopography and untreated polished (control) Ti surfaces. The nanotopography increased cell proliferation and alkaline phosphatase (Alp) activity and upregulated the gene expression of key bone markers of cells grown under both osteogenic and non‐osteogenic conditions. Additionally, the gene expression of α1 and β1 integrins was higher in cells grown on Ti with nanotopography under non‐osteogeneic condition compared with control Ti surface. The higher gene expression of bone markers and Alp activity induced by Ti with nanotopography was reduced by obtustatin, an α1β1 integrin inhibitor. These results indicate that α1β1 integrin signaling pathway determines the osteoinductive effect of nanotopography on MSCs. This finding highlights a novel mechanism involved in nanosurface‐mediated MSCs fate and may contribute to the development of new surface modifications aiming to accelerate and/or enhance the process of osseointegration. J. Cell. Biochem. 115: 540–548, 2014. © 2013 Wiley Periodicals, Inc.
Synthesis and Photocatalytic Properties of Nano‐crystalline In2O3
Authors: Dai Yong‐Ming , Lee Wen‐lian William , Lin Wei‐Chieh , Chen Chiing‐Chang 
Published: October 2013

AbstractA nano‐crystalline In2O3 was synthesized using calcinations methods and was used as a photocatalyst to degrade sulfan blue (SB) dye. In addition, this study addresses the conditions of the degradation and the factors that influenced the catalysis. In2O3 was prepared by calcining In(OH)3 at heat ranges of 100–700 °C for 24 h. The In2O3 was characterized using field emission scanning electron microscopy (FE‐SEM), an X‐ray diffractometer (XRD), thermogravimetric analysis (TGA), and high‐resolution X‐ray photoelectron spectroscopy (HR‐XPS). The activities of these samples were tested for the photocatalytic degradation of SB dye. The results indicated that the In(OH)3 that was calcined at 300 °C for 24 h had the best performance.
Development of Voltammetric Sensor for Determination of Tryptophan Using MWCNTs‐modified Sol‐gel Electrode
Authors: Majidi Mir Reza , Salimi Arezu , Alipour Esmaeel 
Published: October 2013

AbstractIn the present work, an electrochemical sensor was developed for simple and sensitive determination of tryptophan (Trp) using multi‐walled carbon nanotubes modified sol‐gel electrode (MWCNTs/SGE). The electrocatalytic oxidation of tryptophan was investigated by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was found that the oxidation peak current of Trp at the MWCNTs/SGE was greatly improved compared with that of the bare SGE. Furthermore, at the MWCNTs/SGE, the anodic peak potential of Trp is shifted about 220 mV to more negative value indicated that modified electrode has better electrocatalytic activity for electro‐oxidation of Trp. The anodic peak currents increased linearly with the concentration of tryptophan in the range of 0.2 × 10−6 to 15 × 10−6 M with a detection limit of 0.139 × 10−6 M (at an S/N = 3).
Orientable Hamilton Cycle Embeddings of Complete Tripartite Graphs I: Latin Square Constructions
Authors: Ellingham M. N. , Schroeder Justin Z. 
Published: October 2013

AbstractIn an earlier paper the authors constructed a hamilton cycle embedding of in a nonorientable surface for all and then used these embeddings to determine the genus of some large families of graphs. In this two‐part series, we extend those results to orientable surfaces for all . In part I, we explore a connection between orthogonal latin squares and embeddings. A product construction is presented for building pairs of orthogonal latin squares such that one member of the pair has a certain hamiltonian property. These hamiltonian squares are then used to construct embeddings of the complete tripartite graph on an orientable surface such that the boundary of every face is a hamilton cycle. This construction works for all such that and for every prime p. Moreover, it is shown that the latin square construction utilized to get hamilton cycle embeddings of can also be used to obtain triangulations of . Part II of this series covers the case for every prime p and applies these embeddings to obtain some genus results.
Mental Health Professionals’ Natural Taxonomies of Mental Disorders: Implications for the Clinical Utility of the ICD‐11 and the DSM‐5
Authors: Reed Geoffrey M. , Roberts Michael C. , Keeley Jared , Hooppell Catherine , Matsumoto Chihiro , Sharan Pratap , Robles Rebeca , Carvalho Hudson , Wu Chunyan , Gureje Oye , Leal‐Leturia Itzear , Flanagan Elizabeth H. , Correia João Mendonça , Maruta Toshimasa , Ayuso‐Mateos José Luís , de Jesus Mari Jair , Xiao Zeping , Evans Spencer C. , Saxena Shekhar , Medina‐Mora María Elena 
Published: October 2013

ObjectiveTo examine the conceptualizations held by psychiatrists and psychologists around the world of the relationships among mental disorders in order to inform decisions about the structure of the classification of mental and behavioral disorders in World Health Organization's International Classification of Diseases and Related Health Problems 11th Revision (ICD‐11).Method517 mental health professionals in 8 countries sorted 60 cards containing the names of mental disorders into groups of similar disorders, and then formed a hierarchical structure by aggregating and disaggregating these groupings. Distance matrices were created from the sorting data and used in cluster and correlation analyses.ResultsClinicians’ taxonomies were rational, interpretable, and extremely stable across countries, diagnostic system used, and profession. Clinicians’ consensus classification structure was different from ICD‐10 and the American Psychiatric Association's Diagnostic and Statistical Manual of Mental Disorders 4th Edition (DSM‐IV), but in many respects consistent with ICD‐11 proposals.ConclusionsThe clinical utility of the ICD‐11 may be improved by making its structure more compatible with the common conceptual organization of mental disorders observed across diverse global clinicians.
Hypoxia Simultaneously Alters Satellite Cell‐Mediated Angiogenesis and Hepatocyte Growth Factor Expression
Authors: Flann K.L. , Rathbone C.R. , Cole L.C. , Liu X. , Allen R.E. , Rhoads R.P. 
Published: October 2013

AbstractSkeletal muscle regeneration is a multifaceted process requiring the spatial and temporal coordination of myogenesis as well as angiogenesis. Hepatocyte growth factor (HGF) plays a pivotal role in myogenesis by activating satellite cells (SC) in regenerating muscle and likely plays a role as a contributor to revascularization. Moreover, repair of a functional blood supply is critical to ameliorate tissue ischemia and restore skeletal muscle function, however effects of hypoxia on satellite cell‐mediated angiogenesis remain unclear. The objective of this study was to examine the role of HGF and effect of hypoxia on the capacity of satellite cells to promote angiogenesis. To characterize the role of HGF, a microvascular fragment (MVF) culture model coupled with satellite cell conditioned media (CM) was employed. The activity of HGF was specifically blocked in SC CM reducing sprout length compared to control CM. In contrast, MVF sprout number did not differ between control or HGF‐deficient SC CM media. Next, we cultured MVF in the presence of CM from satellite cells exposed to normoxic (20% O2) or hypoxic (1% O2) conditions. Hypoxic CM recapitulated a MVF angiogenic response identical to HGF deficient satellite cell CM. Hypoxic conditions increased satellite cell HIF‐1α protein abundance and VEGF mRNA abundance but decreased HGF mRNA abundance compared to normoxic satellite cells. Consistent with reduced HGF gene expression, HGF promoter activity decreased during hypoxia. Taken together, this data indicates that hypoxic modulation of satellite cell‐mediated angiogenesis involves a reduction in satellite cell HGF expression. J. Cell. Physiol. 229: 572–579, 2014. © 2013 Wiley Periodicals, Inc.
Pharmacokinetics of dalfampridine extended release 7.5‐mg tablets in healthy subjects and individuals with mild and moderate renal impairment: An open‐label study
Authors: Samara Emil , Winkle Peter , Pardo Patricia , Henney Herbert R. , Way Susan L. , Brown Eppie , Lee Angela , Blight Andrew R. 
Published: October 2013

AbstractDalfampridine extended release tablets (D‐ER; prolonged‐release fampridine in Europe) are available to improve walking in patients with multiple sclerosis (MS). D‐ER is mainly renally eliminated; the approved 10‐mg twice daily dose is contraindicated in the United States in patients with moderate or severe renal impairment. This study evaluated single‐dose and steady‐state pharmacokinetics of a 7.5‐mg dose of D‐ER in healthy subjects (n = 13) and subjects with mild (n = 17) and moderate (n = 12) renal impairment. D‐ER plasma concentrations were consistently higher in subjects with renal impairment relative to healthy individuals with a significant (P < .0001) inverse linear relationship between creatinine clearance and drug exposure. Steady‐state AUC0–12 among healthy subjects, 167.0 ± 55.3 ng h/mL, increased 74% and 151% with mild and moderate renal impairment, respectively. The overall incidence of adverse events was 61.5%, 47.1%, and 33.3% in healthy subjects, and subjects with mild and moderate renal impairment, respectively, and for treatment‐related adverse events the rates were 0%, 17.6%, and 8.3%, respectively. The most common adverse events were headache, dizziness, and arthralgia. The pharmacokinetics of D‐ER 7.5‐mg twice daily in subjects with mild renal impairment was comparable to 10‐mg twice daily in patients with MS who had normal renal function. Exposure was significantly higher in moderate renal impairment.
Prediction of overall survival or progression free survival by disease control rate at week 8 is independent of ethnicity: Western versus Chinese patients with first‐line non‐small cell lung cancer treated with chemotherapy with or without bevacizumab
Authors: Claret Laurent , Gupta Manish , Han Kelong , Joshi Amita , Sarapa Nenad , He Jing , Powell Bob , Bruno René 
Published: October 2013

AbstractCategorizations of best response observed at week 8 (between week 3 and 14) of first‐line treatment in two studies of bevacizumab plus chemotherapy in Western (878 patients) and Chinese (198 patients) patients with non‐small cell lung cancer were assessed together with baseline prognostic factors in multivariate parametric models to predict overall survival (OS) and progression free survival (PFS). Predictive performances of the models were assessed by simulating multiple replicates of the studies. Disease control rate (DCR) was the best response categorization to predict OS and PFS. In the OS model, DCR fully captured bevacizumab effect. For PFS, DCR did not fully capture bevacizumab treatment effect. The models adequately predicted OS and PFS distributions in each arm as well as bevacizumab hazard ratio (HR) for OS and PFS, for example, in Western patients (model prediction [95% prediction interval]: 0.84 [0.71–0.98] vs. observed: 0.77 for OS and 0.59 [0.49–0.72] vs. observed: 0.58 for PFS). Covariates in the models captured endpoint differences seen in Chinese patients. There was no impact of Chinese ethnicity on the DCR relationship to OS or PFS. DCR predicted OS benefit with bevacizumab in first‐line NSCLC patients. Western data can be used to inform design of studies in Chinese patients.
Effect of dosing schemes of amoxicillin on eradication rates of Helicobacter pylori with amoxicillin‐based triple therapy
Authors: Furuta Takahisa , Sugimoto Mitsushige , Yamade Mihoko , Uotani Takahiro , Sahara Shu , Ichikawa Hitomi , Yamada Takanori , Osawa Satoshi , Sugimoto Ken , Watanabe Hiroshi , Umemura Kazuo 
Published: October 2013

AbstractObjectivesIn standard regimens for Helicobacter pylori infection, amoxicillin is dosed twice daily, although the bactericidal effect of amoxicillin depends on the %time‐above‐MIC. We aimed to examine whether dosing schemes of amoxicillin influenced eradication rates of amoxicillin‐based regimens.MethodsOne hundred eighty‐seven patients infected with clarithromycin‐sensitive strains of H. pylori were treated with PPI, clarithromycin 200 mg bid and amoxicillin 750 mg bid, 500 mg tid or 500 mg qid for 1 week and 125 infected with clarithromycin‐resistant strains were treated with PPI, metronidazole 250 mg bid and amoxicillin 750 mg bid, 500 mg tid or 500 mg qid for 1 week.ResultsEradication rates (ITT) of the triple PPI/amoxicillin/clarithromycin therapy with bid, tid and qid dosings of amoxicillin were 77.8% (49/63), 93.5% (58/62), and 91.9% (57/62), respectively. Those of the triple PPI/amoxicillin/metronidazole therapy were 80.5% (33/41), 90.5% (38/42), and 95.2% (40/42), respectively. Eradication rates in regimens with tid and qid dosing of amoxicillin were higher than those of regimens with the bid dosing of amoxicillin.ConclusionsThe dosing scheme of amoxicillin significantly influenced eradication rates of triple therapies. Although amoxicillin is empirically dosed twice daily, amoxicillin should be dosed at least three times daily in amoxicillin‐based triple therapies.
Esterification of renewable levulinic acid to ethyl levulinate biodiesel catalyzed by highly active and reusable desilicated H‐ZSM‐5
Authors: Nandiwale Kakasaheb Y. , Niphadkar Prashant S. , Deshpande Shilpa S. , Bokade Vijay V. 
Published: October 2013

AbstractBACKGROUNDIn the near future, fossil fuel will have limitations in terms of availability and also great concerns over its environmental impact. New routes and related technologies based on renewable feedstocks can overcome most of these problems associated with fossil fuel. Among current biodiesel sources, ethyl levulinate (EL) biodiesel obtained from catalytic esterification of renewable levulinic acid (LA) with ethanol has received a great deal of attention. The use of desilicated H‐ZSM‐5 (DH‐ZSM‐5) as heterogeneous acid catalyst for EL biodiesel production in a closed system (under autogeneous pressure) was studied.RESULTSThe effect of reaction parameters such as ethanol to LA molar ratio (4:1 to 10:1), catalyst to LA ratio (0.10–0.25), speed of agitation (100–400 rpm), particle size (53–355 µm), reaction temperature (363–403 K) and reaction time (7 h) was investigated to maximize LA conversion.CONCLUSIONLevulinic acid conversion reached 95% over DH‐ZSM‐5 and the catalyst was reusable for up to six cycles. This LA conversion and the catalyst reusability values are higher than others reported in the literature. A pseudo‐homogeneous (P‐H) kinetic model indicated that reaction rate constants increased with increasing molar ratio, catalyst to LA ratio and reaction temperature. The activation energy decreased from 73.14 to 21.08 kJ mol‐1 when increasing the catalyst to LA ratio from 0.10 to 0.25, which implies a kinetically controlled reaction. © 2013 Society of Chemical Industry
Use of carbon dioxide as feedstock for chemicals and fuels: homogeneous and heterogeneous catalysis
Authors: Dibenedetto Angela , Angelini Antonella , Stufano Paolo 
Published: October 2013

AbstractCO2is considered to play a key role in an eventual climate change, due to its accumulation in the atmosphere. The control of its emission represents a challenging task that requires new ideas and new technologies. The use of perennial energy sources and renewable fuels instead of fossil fuels and the conversion ofCO2into useful products are receiving increased attention. The utilization ofCO2as a raw material for the synthesis of chemicals and fuels is an area in which scientists and industrialists are much involved: the implementation of such technology on a large scale would allow a change from a linear use of fossil carbon to its cyclic use, mimicking Nature. In this paper the use ofCO2as building block is discussed.CO2can replace toxic species such as phosgene in low energy processes, or can be used as source of carbon for the synthesis of energy products. The reactions with dihydrogen, alcohols, epoxides, amines, olefins, dienes, and other unsaturated hydrocarbons are discussed, under various reaction conditions, using metal systems or enzymes as catalysts. The formation of products such as formic acid and its esters, formamides, methanol, dimethyl carbonate, alkylene carbonates, carbamic acid esters, lactones, carboxylic acids, and polycarbonates, is described.The factors that have limited so far the conversion of large volumes ofCO2are analyzed and options for large‐scaleCO2catalytic conversion into chemicals and fuels are discussed. Both homogeneous and heterogeneous catalysts are considered and the pros and cons of their use highlighted. © 2013 Society of Chemical Industry
Effects of crystal habit modifiers on the morphology of calcium sulfate dihydrate grown in strong CaCl2‐HCl solutions
Authors: Feldmann Thomas , Demopoulos George P. 
Published: October 2013

AbstractBACKGROUNDThis work deals with the effect of several additives on the morphology of calcium sulfate dihydrate (DH) crystals, produced by reactive crystallization involving strong CaCl2–HCl solutions. Experiments were conducted at 40 °C under various conditions, namely homogeneous and seeded (heterogeneous) crystallization in CaCl2/HCl solutions ranging from very dilute (0.42 mol L−1/0.04 mol L−1) to very concentrated (1.8 mol L−1/6.3 mol L−1).RESULTSIn the absence of additives a distinct difference in crystal morphology was observed depending on solution composition. Homogeneously formed DH crystals from dilute solution were in the form of needles. DH crystals formed in concentrated acid solution were thick elongated plates. The most influential additives in concentrated solutions were CTAB (cetyltrimethylammoniumbromide), PAA (polyacrylic acid) and PVS (polyvinyl sulfonic acid). CTAB favoured formation of industrially interesting thick slab crystals. In contrast PAA favoured the formation of elongated and thinner crystals as a result of preferential crystal plane adsorption. PVS had a detrimental effect on DH crystal nucleation and growth explained by its polymeric structure.CONCLUSIONApart from PVS, CTAB and PAA no influence was seen for the majority of additives tested. This was attributed to lack of ionization caused by the highly acidic solutions. © 2013 Society of Chemical Industry