bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo analysis of protein quality control in response to aging using transgenic mice
Authors: MARQUES C , MATAFOME P , SANTOS A , LOBO C , SHANG F , PEREIRA P , GIRAO H 
Published: November 2013

AbstractPurpose It has been suggested that aging affects the cells ability to protect protein integrity, which is essential for cellular homeostasis. On cell aging there is impaired protein homeostasis, which can lead to protein insolubilization that is toxic to cells. The carboxyl terminus of Hsp70‐interacting protein (CHIP) is an important ubiquitin ligase for protein quality control, and particularly in selective degradation of aggregation prone proteins. The objective of this study was to characterize the transgenic mice that overexpress CHIP/Ubc5 in lens epithelial cells and investigate the role of these proteins in the process that maintains proteins in solution and therefore participates in a number of age‐related diseases, including cataract.Methods To overexpress CHIP, the α‐crystallin promoter was used to drive the expression of CHIP‐Ubc5 in lens epithelial cells of transgenic mice. The CHIP levels of transgenic mice (Tg) were determined with western blotting analysis. Cataract was induced by single subcutaneous injection of sodium selenite and the lens transparency was assessed by slit lamp examination. The soluble and insoluble protein content in all groups was evaluated.Results The data show that the transgenic mice only overexpress the transgene in lens epithelial cells.The slit lamp analysis showed that transgenic mice appears developed less cataract compared with wild type, with concomitantly increase in ratio of the soluble and insoluble proteins.Conclusion Together the data suggest that overexpression of CHIP/Ubc5 in lens epithelial cells could prevent cataract formation by promoting reduction of protein insolubilization.
Comparison of different techniques to evaluate the biomechanics of the accommodative apparatus in human eyes
Authors: PINILLA L , BURD HJ , MONTENEGRO GA , MIKIELEWICZ M , BARRAQUER RI , MICHAEL R 
Published: November 2013

AbstractPurpose For the study of the biomechanical properties of lens and zonules in a stretching device, we wanted to investigate the effect of keeping the ciliary body intact or cutting it radially.Methods We studied the anterior eye segment of 3 pairs of presbyopic human donor eyes (ages 51‐85). These segments, comprising of lens, zonules, ciliary body and sclera, were radially stretched in our device which allows force and tissue elongation measurements to be made. First, each pair (OD and OS) was stretched with the ciliary body intact and the lens in place, afterwards, in OD, the ciliary body was cut radially and the lens was kept, and in OS, the ciliary body was kept intact and the lens removed, then we stretched again. Forces are given at 500 µm stretch, measured from lens centre to ciliary body edge.Results The mean force with the ciliary body intact and the lens in place was 74 mN. When the ciliary body was cut (with lens in place) the required force was 35 mN. When the ciliary body was keep intact and the lens removed we measured a force of 47 mN, which represents the contribution of the circumferential stretch of the ciliary body.Conclusion Stretching anterior eye segments showed that the required forces were considerably higher when the ciliary body was kept intact as compared when it was cut radially. These forces were increased due to the circumferential resistance by the ciliary body.
Quality of vision with traditional monovision versus modified monovision
Authors: VANDERMEER G , LEGRAS R , GICQUEL JJ , PISELLA PJ 
Published: November 2013

AbstractPurpose To compare binocular subjective quality of vision with modified monovision (MMV) (ie different combinations of spherical (SA4) and secondary spherical (SA6) aberration on each eye) versus traditional monovision (TMV) with different level of anisometropiaMethods A numerical eye model was used to generate images (ie three 0.4 logMAR high contrast letters) viewed through a 4.7mm pupil, degraded by various condition of SA and for proximities from ‐5D to 5D (Naked eye, SA4‐0.4μm, SA4+0.4μm, SA4‐0.4 and SA6+0.2μm, SA4+0.4 and SA6‐0.2μm). Binocular vision was simulated using a 3D‐NVIDIA video device projecting different image on each eye. Binocular through‐focus (TF) quality of vision was evaluated using a grading scale (ITU‐R 500 recommendation) by four subjects with TMV for different level of anisometropia (ie 0.5 to 3D at 0.5D step) and with various combination of MMVResults Area under TF curve (ie a way to evaluate quality of vision) measured for a fair or higher image quality was increased with TMV and MMV compared to naked eye because of the summation of the two best monocular curves. With TMV, a 2.5D anisometropia was the more effective to increase area under TF curve with however a lack of intermediate vision. With MMV the greatest increase of quality of vision was obtained with reverse profile of SA4 and SA6 on each eye (ie SA4‐0.4 and SA6+0.2μm on one eye and SA4+0.4 and SA6‐0.2μm on the other eye). Compared to TMV, reverse profiles of MMV showed a significant benefit in improving area under TF curve thanks to a better quality of intermediate visionConclusion MMV especially with reverse profiles of SA improved overall quality of vision without the lack of intermediate quality of vision intermediate quality of vision induced by TMV
Synchronous malignant transformation of bilateral symmetrical retinocytoma
Authors: DE FRANCESCO S , DE LUCA M , GALIMBERTI D , CAINI M , HADJISTILIANOU T 
Published: November 2013

AbstractPurpose To report the outcome of a 4‐year‐old girl who was found to have bilateral malignant transformed retinocytoma.Methods The patient was treated with systemic chemotherapy and photocoagulation followed by intraarterial chemotherapy.Results Complete remission was achieved.Conclusion Chemotherapy destroyed malignant retinoblastoma and the “retinoma” component remained unchanged.
Müller cell response during degenerative retinopathy in mice
Authors: MENDES‐JORGE L , VALENÇA A , PIRES VMR , CATITA J , RAMOS D , LOPEZ‐LUPPO M , NACHER V , NAVARRO M , CARRETERO A , RODRIGUEZ‐BAEZA A , RUBERTE J 
Published: November 2013

AbstractPurpose Structural retinal alterations were evaluated in a murine model of retinopathy with photoreceptor degeneration, in order to establish response patterns for each stage of the inflammatory process.Methods In this work, a murine model of retinopathy with photoreceptor degeneration was studied. Groups of 4 animals were intraperitoneally injected with 100 mg/Kg of sodium iodate, and euthanized 24, 48 and 72 hours after injection. A non‐injected animal was used as a control in each group. The retinas were analyzed by means of histological techniques, immunohistochemistry, Western Blotting and q‐RT‐PCR.Results In the retina, aggressive stimuli are known to induce reactive gliose. In our murine model of retinopathy, Müller cell activation was accompanied by cell proliferation and glial fibrillar acidic protein (GFAP) over‐expression with maximum values 48h after injection.Conclusion The differences in GFAP expression allowed us to establish expression patterns of this protein which may be used to identify the phase of the inflammatory response in progress.
Secondary glaucoma in familial amyloid polyneuropathy
Authors: ROUSSEAU A , BARREAU E , KASWIN G , CAUQUIL C , THEAUDIN M , ADAMS D , LABETOULLE M 
Published: November 2013

AbstractPurpose To describe the clinical features of secondary glaucoma associated with transthyretin (TTR)‐related Familial Amyloid Polyneuropathy (FAP).Methods In this retrospective monocentric study, 5 patients with FAP associated secondary glaucoma were seen at the ophtalmologic consultation of the french national center for FAP between 2011 and 2012. The mutation of the amyloidogenic TTR variants was analysed for all patients. All patients had a complete ophthalmic examination including BCVA, IOP, slit lamp and optic disc photographs, gonioscopy, pachymetry, automated perimetry, and OCT‐RNFL. Glaucomatous optic neuropathy was diagnosed based on the presence of visual field abnormalities, neuroretinal rim thining, excavation or RNFL defects. Medical and surgical treatments were analysed for all patients.Results All cases had bilateral involvement except 2 monophtalmic patients. All patients were of portuguese origin and carriers of the Val30Met mutation. There were 4 women and 1 man with a mean age of 58.8±7.7 years. Mean BCVA was 0,83±0.9 LogMAR. Three eyes had a BCVA below 20/400. Mean IOP was 26.6±6.6 mmHg. Mean deviation was ‐17.6±11.2dB. Fringed pupil and anterior chamber amyloid deposition were noted in all affected eyes. Three patients had concomitent vitreous involvement. Patients were treated with 1.2±1.5 ocular hypotensive drugs. Four eyes had been treated with at least 1 filtrating surgery.Conclusion FAP‐associated secondary glaucoma is a very severe disease, associated with amyloid deposits in the anterior chamber and characteristic pupil deformation. Systematic and comprehensive eye examination should be performed in all patients affected with FAP in order to improve early detection of glaucoma.
Effectiveness of the glaucoma screening in employees of the University Hospital St. Luc, UCL, in Brussels
Authors: STINGLHAMBER A , POURJAVAN S 
Published: November 2013

AbstractPurpose To assess the effectiveness of free glaucoma screening for the employees of University Hospital St. Luc, UCL, Brussels, during the World Glaucoma Week 2013.Methods During 1 week, the employees in the university hospital St.Luc, were invited to Glaucoma outpatients' free screening consultation. 320 responded positively. The participants filled before the examination a questionnaire: age, sex, personal ocular trauma or surgery, and familial history was asked. The subjects were examined by an ophthalmologist in training and supervised by an experienced glaucoma consultant if needed (SP). The examination of anterior segment (Van Herick’s, depth, Krukenberg’s spindle, iris transillumination and pseudoexfolliation), the optic disc (size, rim and peripapillary hemorrhages) and the IOP by GAT were performed. Depending of clinical examination, a visual field test was performed. Finally, according to the results, the examiner classified the subjects in ‘Normal’, ‘suspect’ and ‘Glaucoma’ group. A second appointment was scheduled for the patients in the ‘Suspect’ or ‘Glaucoma’ classification.Results From the 640 eyes, there were in total 15% in ‘suspect group’ classified. The aspect of their optic disc and a suspect visual field was the main reason for this classification.5% of the total eyes undergone a preventive iridotomy, none of these patients had experienced any ocular symptoms. 3% of all eyes showed glaucoma with structural and functional glaucomatouse damage.Conclusion Glaucoma is still not widely known. Even for the employees of a large university hospital white easy access to the care, is screening important. The preventive iridotomy was the best result of this WGW screening.
Comparing the use of steroid vs artifitial tears following selective laser trabeculoplasty
Authors: ALI ALJASIM L 
Published: November 2013

AbstractPurpose To compare between using potent steroid, mild steroid or just artificial tears following selective laser trabeculoplasty (SLT) , and its effect on outcomeMethods Retrospective file review of all patients underwent SLT in our institute for a year, the data divided into 3 groups: group 1 received Prednisolone acetate 1% ophthalmic suspension ± their glaucoma medications, in the immediate post laser period, group 2 had Fluorometholone eye drops, and group 3 had artificial tears. The rate of complication & outcome was compared between the groups.Results 215 eyes of 129 patients underwent SLT, from October 2012‐October 2013. During the post‐laser period, Prednisolone acetate 1% was used in 134 eyes, for 8.79±3.6days (3‐14 days), Fluorometholone for 41 eyes for 6.85±2.1 (3‐14 days) & 40 patients had neither. Most of the eyes had 360* treatment, half of the patients had DM & 40% had hypertension Pre‐SLT intraocular pressure(IOP) in group 1 was 19±6mmHg on 2.4 glaucoma medications, in group 2 19.4±5.4mmHg on 2.4 medications & in group 3 was 21±6.2mmHg on 2.5 medications. The first group treated with 78.6±13.2 shots of 0.56mj per shot, group 2 with 81.1±16.6 shots of 0.59mj, and group 3 with 75.9±15.2 of 0.59mj. The resulted decrease of IOP in the first group was 2.4±5.3mmHg (12.7%), in group 2 was 3.4±4.8mmHg (17.7%) & in the last group was 5.5±6.1mmHg (26.1%). One patient had persistent anterior chamber reaction for more than 2 week, in both eyes, in the first group( that was treated with prednisolone). In the other groups, no eye showed any degree or AC reaction.Conclusion The use of steroid had an adverse effect on the result of SLT, while the use of artificial tears post‐SLT did not result in increased rate of complication.
Ganglion cell complex measurement and glaucoma diagnosis using two SD‐OCT
Authors: FRANCOZ M , EL CHEHAB H , FENOLLAND JR , DELBARRE M , MARéCHAL M , OUANEZAR S , GIRAUD JM , MAY F , RENARD JP 
Published: November 2013

AbstractPurpose Macular retinal ganglion cell analysis from Cirrus HD‐OCT (Carl Zeiss Meditec, Dublin, CA) and RTVue‐OCT (Optovue Inc., Fremont, USA) were evaluated in their glaucoma diagnostic ability.Methods A total of 169 eyes were enrolled in 3 groups: (1) 70 early primary open angle glaucoma patients (POAG), (2) 44 moderate‐to‐advanced POAG, (3) 55 normal subjects. All patients underwent a complete examination and a macular analysis using GCA algorithm from Cirrus HD and GCC algorithm from RTVue. Macular average, minimum and 6 sectoral GCL‐IPL thicknesses obtained with Cirrus and average, inferior and superior GCC thicknesses and FLV and GLV obtained with RTVue were analyzed. Each eye underwent cpRNFL analysis using Cirrus and RTVue. Glaucoma discrimination ability was assessed using area under the receiver operator characteristic curve (AUC) for all parameters.Results Average GCL‐IPL thickness with Cirrus was 65.6 ± 7.4 μm, 57.8 ± 13.1 μm and 80.3 ± 9.9 μm and average GCC with RTVue was 75.7 ± 10.7 μm, 72.6 ± 9.7 μm et 93.9 ± 7.7 μm, for group 1, 2 and 3 respectively (p<0.05 between each group). AUCs ranged from 0.836 to 0.925 for Cirrus macular parameters and from 0.878 to 0.949 for RTVue macular parameters. These values were compared to the discrimination ability of cpRNFL parameters from each OCT.Conclusion The two OCT macular ganglion cell analysis showed similar glaucoma diagnostic ability and were comparable with that of cpRNFL. Specific macular parameters: GCL‐IPL minimum, GCL‐IPL inferotemporal, FLV and GLV have a high diagnosis performance.
Analyse of segmented macular inner layers by spectral domain optical coherence tomography in glaucoma
Authors: DELBARRE M , EL CHEHAB H , FRANCOZ M , ZERROUK R , MARECHAL M , OUANEZAR S , GIRAUD JM , MAY F , RENARD JP 
Published: November 2013

AbstractPurpose To evaluate the glaucoma discriminating ability of macular inner layers measured by SD‐OCT (Cirrus HD‐OCT, Carl Zeiss Meditec (CZM) Dublin, CA, USA).Methods Our study concerns 252 primary open angle glaucoma (POAG) eyes (early, moderate and advanced glaucoma) and 337 normal eyes which underwent a visual field testing (Humphrey Field Analyser, SITA‐Standard 24‐2, CZM), and Cirrus HD‐OCT imaging (macular and optic disc cube 200x200). OCT macular scans were segmented into macular nerve fibre layer (mNFL), ganglion cell layer with inner plexiform layer (GCIPL) and ganglion cell complex (GCC=GCIPL+mNFL). Area under the receiver operator characteristic curve (AUC) for macular parameters and mean circumpapillary retinal nerve fibre layer (cpRNFL) were used.Results There is a statistically significant difference between glaucomatous and control eyes for all parameters (p <0.05). For early POAG group the best AUCs were respectively minimum GCIPL (0.747), average GCIPL (0.716), GCC (0.71), cpRNFL (0.704), minimum mNFL (0.673) and average mNFL (0.655). For moderate POAG group the best AUCs were respectively minimum GCIPL (0.864), GCC (0.858), cpRNFL (0.853), average GCIPL (0.838), average mNFL (0.82) and minimum mNFL (0.815). For advanced POAG group the best AUCs were respectively minimum GCIPL (0.938), GCC (0.928), minimum mNFL (0.92), average GCIPL (0.908), cpRNFL (0.854) and average mNFL (0.854).Conclusion Minimum GCIPL would have the highest sensitivity to diagnosis glaucoma. It may represent an interesting complementary parameter to cpRNFL thickness.
Correlation between retinal ganglion cell count and morpho‐functional parameters in glaucoma
Authors: MILANO G , LOMBARDO S , DISTANTE P , TINELLI C , LANTERI S , VERTICCHIO VERCELLIN AC , CUTOLO CA , RAIMONDI M , ROLANDO M , ROSSI GCM 
Published: November 2013

AbstractPurpose To evaluate the correlation between retinal ganglion cell (RGC) count and morpho‐functional parameters provided by spectral‐domain optical coherence tomography (SD‐OCT) and standard automated perimetry (SAP)Methods 52 eyes evaluated for open angle glaucoma underwent a complete ophthalmologic examination including SAP and SD‐OCT evaluation of retinal nerve fiber layer (RNFL) thickness and macular ganglion cell complex (GCC). RGC counts were obtained using a previously derived algorithm proposed by Medeiros et al. Patients were divided into 3 groups according to glaucoma severity staged by Glaucoma Staging System 2: suspects (stage 0‐border), early (stage 1‐2) and manifest glaucoma (stage 3‐4‐5)Results Average RGC count estimate was 933803±132506 in suspects, 685583±158345 in early and 385316±185374 in manifest glaucoma (p<0.001). The same trend was noticed for the considered parameters, even if the major loss was found between early and manifest glaucoma in functional data (MD and PSD) and between suspects and initial glaucoma in structural data (RNFL and GCC). RGC count showed very poor correlation with PSD in all groups (r<‐0.3), a progressively increased correlation with MD according to glaucoma severity (r>0.3 in suspects, r>0.9 in manifest glaucoma), a moderate correlation with RNFL (r>0.6) and GCC (r>0.5) in all groupsConclusion A substantial loss of RGCs is detectable in all groups and proportional to glaucoma stages. Structural evaluation of optic nerve (RNFL) and inner macular retina (GCC) can provide a useful diagnostic tool in all stages while SAP is well correlated only to substantial loss of RGC. Macular RGC are involved in early glaucoma loss
Ocular Pulse Amplitude is associated with ocular blood flow velocities in healthy subjects and patients with primary open angle glaucoma, but not normal tension glaucoma
Authors: PINTO LA , VANDEWALLE E , STALMANS I 
Published: November 2013

AbstractPurpose Purpose: to determine the correlation between ocular blood flow changes throughout the cardiac cycle on ocular pulse amplitude (OPA) in glaucoma patients and healthy subjects.Methods Method: A prospective, case‐control study was designed with dynamic contour tonometry and color Doppler Imaging performed in healthy controls and glaucoma patients. An offline waveform analysis of the Doppler signal was performed by a masked observer for each of the retrobulbar arteries. Linear regression models using intraocular pressure (IOP), age and Doppler waveform variables [(resistance index (RI) and systolic/diastolic velocity ratio (Sm/Dm)] were designed to identify independent variables associated with OPA.Results Results: 192 patients were included [healthy controls: 55; normal tension glaucoma (NTG): 63, primary open‐angle glaucoma (POAG): 74]. OPA showed a significant associated with the constructed model in healthy individuals and in POAG patients (r=0.54, p<0.001; r=0.43, p<0.001, respectively). Short ciliary arteries flow variables were the only vascular‐related parameters independently associated with OPA in these groups (p<0.05). IOP was independently associated with OPA in the POAG group (p<0.001), but not the healthy group (p=0.48). OPA in the NTG group did not correlate with the constructed model (r=0.24, p=0.21).Conclusion Conclusions: OPA seems to reflect blood flow changes in the short ciliary arteries in healthy individuals and POAG patients, but not in NTG patients. In the latter, the ocular pulse may relate to other variables than blood flow velocities.
Distribution of ocular biometric parameters during the eye growth
Authors: YEKTA A , HASHEMI H , OSTADIMOGHADDAM H , JAFARZADEHPOUR E , KHABAZKHOOB M , REZVAN F 
Published: November 2013

AbstractPurpose To determine the distribution of ocular biometric parameters and their correlation with age and gender during the eye growth.Methods In a cross‐sectional study, a multi‐stage stratified cluster sampling was used to sample the study population from Dezful schools, a city in the southwest of Iran. Participants were selected from the all school grades and both genders. Examinations were performed after obtaining consent from the subjects's parent. LENSTAR/BioGraph (WaveLight AG, Erlangen, Germany) was used for ocular biometry.Results Of 864 selected subjects, 683 participated in the study (response rate:79.1%); 377 participants (55.2%) were males. Mean age of the subjects was 12±3.4 years (range: 6.0‐18 years). Mean and 95% confidence interval(CI) of axial length (AL),anterior chamber depth (ACD), lens thickness (LT),central corneal thickness (CCT),corneal diameter (CD), and pupilary diameter(PD) were 23.13 mm (22.93‐23.33), 3.01 mm (2.96‐3.06), 3.58 mm (3.55‐3.61), 549.33 mic (546.59‐552.07), 12.34 mm (12.31‐12.38), and 4.97 (4.91‐5.03), respectively. Ocular biometric parameters were normally distributed. Distribution of LT and CD were leptokurtic and the distribution of the PD was platykurtic. The mean of AL, ACD, CD and CR were significantly higher in boys and the mean of LT was significantly higher in girls. Axial length and ACD increased and the LT decreased significantly with age. Axial length and CR had the highest correlation (r=0.669) and ACD had the strongest correlation with theConclusion The results of this study indicated that AL and ACD were intermediate and shorter respectively. Corneal diameter was bigger in comparison with the other studies. The majority of the changes were observed in AL and LT.
Interleukin‐8 promoter polymorphism is associated with the initial repose to bevacizumab in AMD treatment
Authors: IMMONEN I , HAUTAMÄKI A , KIVIOJA J , VAVULI S , KAKKO S , SAVOLAINEN ER , SAVOLAINEN M , LIINAMAA J , SEITSONEN S , ONKAMO P , JÄRVELÄ I 
Published: November 2013

AbstractPurpose To study the association of potential key single‐nucleotide polymorphisms with the short‐term anatomic response to bevacizumab treatment in exudative age‐related macular degeneration (AMD).Methods Clinical data of 96 bevacizumab‐treated exudative age‐related macular degeneration patients were analyzed retrospectively. Blood DNA was collected. Based on the disappearance of intra‐ or subretinal fluid in optical coherence tomography, patients were graded as responders, partial responders, or nonresponders after 3 initial treatment visits and a median time of 3.5 months. Representative single‐nucleotide polymorphisms of interleukin 8, vascular endothelial growth factor, erythropoietin, complement factor H, complement component C3, and LOC387715 genes were analysed.Results Interleukin 8 promoter polymorphism −251A/T,conferring a more acitve interleuking‐8 system, was significantly associated with persisting fluid in optical coherence tomography. The A allele was more frequent in nonresponders than in responders (P = 0.033). In multivariate modeling, the AA genotype of −251A/T (P = 0.043) and occult (P = 0.042) or predominantly classic (P = 0.040) lesions predicted a poorer outcome.Conclusion The interleukin ‐8 pathway may modulate the early anatomic response to anti‐VEGF treatment in AMD. A possible activation of interleukin ‐8 production in patients may represent a compensatory mechanism to chronic blockade of VEGF signalling in AMD lesions