bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feline malignant lymphoma in an uncommon location as a differential diagnosis for neurological disease
Authors: Lépine Maximilien , Schmitz Sarah , Körber Svenja , Köhler Kernt 
Published: February 2025

Case summary A 12-year-old male castrated domestic shorthair cat exhibited right Horner’s syndrome, right facial nerve paresis, difficulty swallowing, coughing, gait abnormalities and weight loss. Despite prior unspecific treatment by a primary care veterinarian with cortisone and antibiotics, the cat’s condition worsened, culminating in tetraparesis and right hemispasms. Imaging studies, including CT and MRI, identified a mass extending from the carotid body into the neurocranium, causing displacement of adjacent brain structures and meningeal contrast uptake. Histopathology confirmed a malignant B-cell lymphoma. Differential diagnoses are explored, with a particular focus on carotid body tumours, which originate from the chief cells of the carotid body. These neoplasias are rare in non-human primates, dogs, cats and horses, possibly influenced by genetic predisposition and environmental factors such as hypoxia. Relevance and novel information Carotid body tumours are rare in cats, as they are in other animal species. Although lymphomas are the most common feline neoplasms, to our knowledge, no previous case of a B-cell lymphoma in the carotid body has been described in the feline species to date. This case underscores the importance of considering rare and common neoplastic entities in feline patients with atypical clinical presentations and locations. Thereby highlighting the diagnostic challenges in veterinary oncology.
The striatal matrix compartment is expanded in autism spectrum disorder
Authors: Waugh Jeff L. , Hassan Asim O. A. , Funk Adrian T. , Maldjian Joseph A. 
Published: February 2025

Abstract

Background
Autism spectrum disorder (ASD) is the second-most common neurodevelopmental disorder in childhood. This complex developmental disorder manifests with restricted interests, repetitive behaviors, and difficulties in communication and social awareness. The inherited and acquired causes of ASD impact many and diverse brain regions, challenging efforts to identify a shared neuroanatomical substrate for this range of symptoms. The striatum and its connections are among the most implicated sites of abnormal structure and/or function in ASD. Striatal projection neurons develop in segregated tissue compartments, the matrix and striosome, that are histochemically, pharmacologically, and functionally distinct. Immunohistochemical assessment of ASD and animal models of autism described abnormal matrix:striosome volume ratios, with an possible shift from striosome to matrix volume. Shifting the matrix:striosome ratio could result from expansion in matrix, reduction in striosome, spatial redistribution of the compartments, or a combination of these changes. Each type of ratio-shifting abnormality may predispose to ASD but yield different combinations of ASD features.

Methods
We developed a cohort of 426 children and adults (213 matched ASD-control pairs) and performed connectivity-based parcellation (diffusion tractography) of the striatum. This identified voxels with matrix-like and striosome-like patterns of structural connectivity.

Results
Matrix-like volume was increased in ASD, with no evident change in the volume or organization of the striosome-like compartment. The inter-compartment volume difference (matrix minus striosome) within each individual was 31% larger in ASD. Matrix-like volume was increased in both caudate and putamen, and in somatotopic zones throughout the rostral-caudal extent of the striatum. Subjects with moderate elevations in ADOS (Autism Diagnostic Observation Schedule) scores had increased matrix-like volume, but those with highly elevated ADOS scores had 3.7-fold larger increases in matrix-like volume.

Conclusions
Matrix and striosome are embedded in distinct structural and functional networks, suggesting that compartment-selective injury or maldevelopment may mediate specific and distinct clinical features. Previously, assessing the striatal compartments in humans required post mortem tissue. Striatal parcellation provides a means to assess neuropsychiatric diseases for compartment-specific abnormalities. While this ASD cohort had increased matrix-like volume, other mechanisms that shift the matrix:striosome ratio may also increase the chance of developing the diverse social, sensory, and motor phenotypes of ASD.
Hypocitraturia and urolithiasis risk: Insights from a 24-h urinary metabolic evaluation in South-Asian stone formers
Authors: Arjun N. , Dhanshekar N. , Kamble Nitin Kumar , Varghese Jofin John , Gaonkar Koustubh Jeevan , Gowda Anoushka , Nagaraj H. K. , Iqbal Tariq 
Published: February 2025

Abstract

Background
Urolithiasis is a chronic, systemic condition, with a high prevalencet and rising incidence. It leads to a lower quality-of-life for patients affected by it—even though asymptomatic. Lithogenesis is a metabolic process where urinary chemical abnormalities promote stone formation in the urinary tract. As a consequence, there needs to be an assessment of urinary chemicals that may prevent stone formation. The purpose of this study was to observe changes in a 24-h urine collection for metabolites to suggest an intervention to reduce the risk of recurrence in stone formers (SFs).

Methods
An observational study performed at a tertiary care hospital, with a cohort of 40 stone formers—after 4 weeks of any intervention and when found to be stone-free, were included in this prospective study. All patients underwent a basic metabolic work-up followed by a single 24-h urine collection which was evaluated for metabolites. Patients were categorized based on the risk for stone formation and critically analyzed.

Results
The mean age was 35 years (range: 9–65). Risk stratification identified 17 (42.5%) as first-time stone formers and 23 (57.5%) as recurrent stone formers. Overall, 27 (67.5%) patients were categorized as high risk for stone formation. 17 (42.5%) had at least one abnormality, the most common (26/40, 65%) being hypocitraturia, with only isolated hypocitraturia in 18 (45%) stone formers. Low urine volume (r = 0.317, p = 0.046) was significantly co-related with low magnesium and citrate levels (r = 0.534, p = 0.001). A solitary patient was identified to have gouty diathesis with no abnormality identified in 12 (30%). The mean body mass index (BMI) was 24.3 ± 3.12 kg/m2 and a higher BMI was significantly associated with high-risk status (χ
2 (2) = 17.43, p < 0.001) for stone formation.

Conclusions
Clinicians should consider dietary modifications as the primary intervention for South-Asian patients with urolithiasis based on findings of hypocitraturia in 24-h urine collections. Future studies should validate dietary interventions to reduce stone recurrence and compare outcomes between South-Asian and Western populations.
Inhalational versus intravenous anesthetic for cerebrovascular accident outcomes after surgical revascularization for adult moyamoya disease
Authors: Cheng Yifei , Zha Chaochao , Che Xuehua , Wang Yingwei 
Published: February 2025

Abstract

Purpose
To compare the effects of inhalational anesthetics and intravenous anesthetics on the neurological function of patients with moyamoya disease (MMD) after vascular bypass surgery.

Methods
The clinical anesthesia data of patients were retrospectively collected. Patients who underwent bypass grafts with general anesthesia from January 1st, 2019, to December 31st, 2020, in Huashan Hospital affiliated with Fudan University, were selected. The primary endpoint was stroke incidence within seven days after anesthesia, and the secondary endpoints included transient neurological deficits (TNDs) and incidence of postoperative Epilepsy.

Results
We compared the data of MMD patients who received inhalational anesthetics (Sevoflurane anesthetics, n = 197, group S) and intravenous anesthetics (Propofol anesthetics, n = 219, group P). The stroke incidence in the two groups (group S vs. group P) was 6.6% vs. 5.9% (OR = 0.893; 95% CI, 0.404–1.976; p = 0.780), and the group S vs. group P of TNDs incidence was 32.5% vs. 31.1% (OR = 0.936; 95% CI, 0.619-0.1.415, p = 0.753). At discharge, anesthetics didn’t affect the neurological endpoint. Intravenous anesthetics provided patients with better hemodynamics compared with inhalational anesthetics during MMD vascular bypass surgery (group S vs. group P, ARVSBP: 6.4 vs. 5.2, p < 0.001, ARVDBP: 3.9 vs. 3.3, p = 0.002, ARVMBP: 4.5 vs. 3.8, p = 0.001,). There were statistical no differences in the NHISS score (S group vs. P group = 2:1, p = 0.082) at 7 days after surgery, but mRS score (S group vs. P group = 2:1, p < 0.001) at 7 days after surgery, as well as the mRS score at 6 months of follow-up (S group vs. P group = 0:0, p < 0.001), although the difference in scores was small.

Conclusion
Our data indicated that both inhalational and intravenous anesthetics had protective effects on patients who underwent MMD bypass grafts. MMD patients who received inhalational anesthetics and intravenous anesthetics had similar odds of neurological deficits. When comparing long-term clinical data, most patients experience good neurological recovery after receiving inhalation or intravenous anesthesia, when compared p75 mRS score(S group vs. P group = 3:1)in 6 month indicate that intravenous anesthetics might be more suitable for patients undergoing MMD bypass grafts. During the operation hemodynamic stability in the propofol group is greater than that in the sevoflurane anesthesia group.
Understanding and supporting the mental health and professional quality of life of academic mental health researchers: results from a cross-sectional survey
Authors: Bennett Jacks , Di Cara Nina , Winstone Lizzy 
Published: February 2025

Abstract

Background
Academic mental health research is critical to understanding, treating and preventing poor mental health. Researchers often have their own lived experience of a mental health condition, but despite potential exposure to distressing research material, the mental health and work-related quality of life of mental health researchers is not systematically supported in UK universities. This study aimed to quantitatively characterise the mental health experiences, professional quality of life and workplace support needs of this group.

Methods
UK academic mental health researchers (n = 254) answered an online survey in March 2024. Using linear regression modelling, we tested associations between socio-demographic, mental and physical health and work-related factors alongside negative and positive aspects of professional quality of life, i.e., secondary traumatic stress, burnout and compassion satisfaction, as well as maladaptive coping strategies such as alcohol and drug use or sickness absence from work. We also compared researchers’ workplace support experience with their perceived support need and examined implications for funding applications.

Results
Having personal lived experience of a mental health condition showed the strongest association with poorer professional quality of life outcomes. Mental health researchers using qualitative methods also reported higher levels of secondary traumatic stress and burnout than those using quantitative methods, as did those with a disability or chronic illness (compared to those without). Researchers with personal lived experience of a mental health condition also showed ten times the odds of taking sickness leave to cope with work related feelings. There were important differences between the types of workplace support researchers experience with those they report needing. Our evidence also points to more guidance needed on factoring support into research projects.

Conclusions
Our findings highlight the wealth of lived experience amongst mental health researchers, and the importance of providing systematic proactive support for this group, as well as for those with a disability or chronic illness, or those using qualitative methodologies. With sickness absence having considerable economic and organisational consequences for employers and funders, recommendations include developing researcher well-being plans, regular end of project debriefs, development and training on challenging topics, and clearer consideration of researcher support in funding applications.
Computational development of mushroom-6-glucan/paclitaxel as a synergistic complementary medicine for breast cancer therapy
Authors: EL-Deeb Nehal M. , Ibrahim Omar M. , Kamel Ayman M. , Gomaa Ahmed I. , Kenawy Ahmed M. 
Published: February 2025

Abstract

Background
Breast cancer is chemo-resistant and highly metastatic, often resulting in patient mortality. One of the primary factors contributing to the metastasis and chemotherapy resistance is the presence of cancer stem-like cells. We posited that the natural polysaccharide known as 6-glucans, derived from Pleurotus ostreatus, could effectively counteract the chemotherapy resistance associated with cancer stem-like cells in breast cancer.

Methods
We computationally developed a specific dual combinatorial therapy involving 6-glucans and Paclitaxel (PTX) and tested on preclinical 3D mammosphere human tumor models representing receptor-positive and receptor-negative breast cancer. Using this preclinical 3D spheroid technology, we tested the anti-cancer properties of these predicted treatment combinations on mammospheres containing human breast cancer stem cells.

Results
Among the 40 distinct combinations examined, computational prediction revealed that the addition of 2.0 mg/mL of 6-glucans to a low dose of 3.0 µg/mL PTX was the sole combination demonstrating a synergistic effect. This optimized synergistic combination therapy displayed a significant inhibitory impact on human cancer epithelial and stem cell migration, evasion, and colony formation. The inclusion of 6-glucans also augmented apoptosis in both breast cancer cells and stem cells, leading to a six-fold reduction in BrdU labeled cells and an increased arrest of cells in the sub-G0 phase. These effects were mediated through mitochondrial dysfunction and the downregulation of associated oncogenes.

Conclusion
Our study revealed that the computationally predicted 6-glucans-based binary complementary medicine exhibited sequence- and concentration-dependent anticancer synergistic effects.
SWOT analysis of the application of three digital media in OLPE physical education teaching: Edmodo, Zoom, and Google Meet
Authors: Gao Yongqi , Zhu Li , Tian Miao 
Published: February 2025

Abstract
Digital education emphasizes the use of technology to enhance and transform teaching, promoting the improvement of instructional models. It is poised to become a significant driving force in advancing high-quality educational development. However, numerous practical challenges remain, such as disadvantages of digital technology resources, ethical norms, and professional training. This study aims to examine the advantages, disadvantages, opportunities and challenges of the three Online physical education(OLPE) teaching modes–Edmodo, Zoom and Google Meet–through literature review, case-based empirical method, survey-based empirical method, mathematical statistics analysis and SWOT analysis. It explores the international OLPE teaching concept, aiming to integrate digital sports teaching within the OLPE model with current traditional teaching practices, and to develop a paradigm better aligned with modern educational methods. The results revealed that based on constructivist learning theory and situational learning, technological enhancements, correct ethics, and the integration of OLPE resources could help advance the development of digital-physical education teaching. The study offers valuable insights into the challenges and disadvantages of OLPE in digital education. It will provide both theoretical and practical guidance for advancing digital teaching practices.
Anemonefish use sialic acid metabolism as Trojan horse to avoid giant sea anemone stinging
Authors: Roux Natacha , Delannoy Clément , Yu Shin-Yi , Miura Saori , Carlu Lilian , Besseau Laurence , Nakagawa Takahiro , Sato Chihiro , Kitajima Ken , Guerardel Yann , Laudet Vincent 
Published: February 2025

Abstract

Background
Anemonefish association with sea anemones is a prime example of mutualistic symbiosis. These fish live inside the sea anemone, benefitting from the protection of its toxic nematocysts, and in return, protect the anemone from its own predators. How anemonefish manage to avoid their host toxic stings remains unclear. One hypothesis suggests that low levels of sialic acids in anemonefish mucus prevent nematocyst discharge.

Results
This study verified four predictions: (i) anemonefish mucus has lower sialic acid levels than non-symbiotic damselfish; (ii) this reduction is specific to mucus; (iii) during development, sialic acid levels inversely correlate with protection; (iv) sea anemone mucus has minimal sialic acids.

Conclusions
We conclude that anemonefish regulates the level of sialic acids in their mucus to avoid nematocyst discharge. We also highlight several genes implicated in sialic acid removal that could explain the protection mechanisms in place. This mechanism, potentially used by Dascyllus trimaculatus juveniles, suggests a convergent strategy for mutualistic associations with sea anemones.
Assessing the adoption of biosecurity measures among extensive livestock producers: a case study in the free-range pig sector of Corsica
Authors: Loeillot Theo , Gisclard Marie , Trabucco Bastien , Charrier François , Jori Ferran , Antoine-Moussiaux Nicolas , Delabouglise Alexis 
Published: February 2025

Abstract

Background
Increasingly exposed to emerging sanitary risks, extensive livestock farming systems are confronted with the imperative of incorporating biosecurity measures in their production models in order to limit the risk of introduction and diffusion of animal pathogens. Yet, ex-ante assessment methods of the likelihood of biosecurity measures implementation are poorly documented. Our study aimed at comparing alternative methods of elicitation of preferences to assess the attitude of extensive livestock farmers towards biosecurity measures. We used, as a case study, the Regional Porcine Sanitary Plan (RPSP) elaborated for the free-range pig sector of Corsica Island to meet the newly established national disease prevention requirements of France in the face of risk of African Swine Fever introduction.

Methods
The RPSP imposed (1) a fencing of the breeding pigs’ area, (2) the neutering of the pigs not used for breeding, and (3) a management process of dead pigs’ carcasses found on pastures. We evaluated four attributes of the sanitary plan, including (1) proportion of the implementation cost covered by state subsidies, (2) mandatory carcass management, (3) people allowed to neuter gilts, (4) the age limit for neutering. We performed interviews of a sample of free-range pig farmers using three methods in parallel, namely (1) direct qualitative elicitation, (2) attributed-based stated choices and (3) semi-quantitative ranking of attributes.

Results
Farmers’ preference for a high subsidization of the sanitary plan and for enforcing the neutering of pigs at an early age was consistent across all used methods. Participants expressed heterogeneous preferences for the two other attributes. Half of the respondents were reluctant to entrust veterinarians with neutering gilts while the other half deemed veterinarians’ intervention compulsory. Contradictory preferences were obtained on rendering carcass management mandatory depending on the elicitation method.

Conclusion
Our study demonstrates the added value of using choice-based methods, where respondents weigh the individual costs and benefits associated with different options, in combination with qualitative or semi-quantitative ranking methods in which farmers express their opinions and give more consideration to their community interest. It also reveals potential issues of heterogeneities among farmers’ preferences that need to be taken into consideration in similar surveys.
A surrogate in vitro experimental model for off-label drug repurposing: inhibitory effect of montelukast on bovine respiratory syncytial virus replication
Authors: Kurucay Hanne Nur , Yazici Zafer , Bayrakal Vahide , Muftuoglu Bahadir , Ozan Emre , Tamer Cuneyt , Gozel Seda , Barry Gerald , Igde Mahir , Okur-Gumusova Semra , Albayrak Harun , Elhag Ahmed Eisa , Baskin Huseyin 
Published: February 2025

Abstract

Background
Repurposing off-label drugs during epidemics or pandemics with unknown/known pathogens, particularly when their side effects and complications are already known, can be a strategic approach, as seen during the COVID-19 pandemic. Developing surrogate in vitro experimental models (passage-to-passage), which mimic epidemic/pandemic-like transmission (human-to-human), may enhance this repurposing process. This study evaluates montelukast sodium (MLS), a US FDA-approved leukotriene receptor antagonist for asthma, to explore its potential repurposing antiviral effects against bovine respiratory syncytial virus (BRSV), which has basic similarities to human respiratory syncytial virus (HRSV) as both belong to the Pneumoviridae family.

Methods
An in vitro serial passage model was developed using MDBK cells infected with a local wild-type strain of BRSV (43TR2018). The cytotoxicity of MLS was assessed via the trypan blue exclusion method, identifying non-toxic concentrations. The impact of MLS on viral spread and infectivity was measured through TCID50 values over 10 passages. Viral loads were confirmed by nested RT-PCR and quantified using qPCR, while apoptosis, necrosis, and nitric oxide production were evaluated through staining and nitrite assays. Data were analyzed using ANOVA and Tukey's test (p < 0.05).

Results
Control cells exhibited 97.16% viability, with 10 µM and 20 µM MLS concentrations maintaining viabilities of 89.2% and 87.3%, respectively. Viral titers significantly decreased at higher concentrations of MLS (up to 99.94% inhibition). Apoptosis rates decreased in MLS-treated cells, and live cell percentages improved, especially at 20 µM. Nitric oxide levels showed no significant differences across groups.

Conclusion
MLS demonstrated a dose-dependent antiviral effect against BRSV, achieving 99% viral inhibition properties in MDBK cells. These promising results warrant further investigation into the antiviral mechanisms of MLS.