bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor Protein Tyrosine Phosphatase α–Mediated Enhancement of Rheumatoid Synovial Fibroblast Signaling and Promotion of Arthritis in Mice
Authors: Stanford Stephanie M. , Svensson Mattias N. D. , Sacchetti Cristiano , Pilo Caila A. , Wu Dennis J. , Kiosses William B. , Hellvard Annelie , Bergum Brith , Muench German R. Aleman , Elly Christian , Liu Yun‐Cai , den Hertog Jeroen , Elson Ari , Sap Jan , Mydel Piotr , Boyle David L. , Corr Maripat , Firestein Gary S. , Bottini Nunzio 
Published: September 2015

ObjectiveDuring rheumatoid arthritis (RA), fibroblast‐like synoviocytes (FLS) critically promote disease pathogenesis by aggressively invading the extracellular matrix of the joint. The focal adhesion kinase (FAK) signaling pathway is emerging as a contributor to the anomalous behavior of RA FLS. The receptor protein tyrosine phosphatase α (RPTPα), which is encoded by the PTPRA gene, is a key promoter of FAK signaling. The aim of this study was to investigate whether RPTPα mediates FLS aggressiveness and RA pathogenesis.MethodsThrough RPTPα knockdown, we assessed FLS gene expression by quantitative polymerase chain reaction analysis and enzyme‐linked immunosorbent assay, invasion and migration by Transwell assays, survival by annexin V and propidium iodide staining, adhesion and spreading by immunofluorescence microscopy, and activation of signaling pathways by Western blotting of FLS lysates. Arthritis development was examined in RPTPα‐knockout (KO) mice using the K/BxN serum–transfer model. The contribution of radiosensitive and radioresistant cells to disease was evaluated by reciprocal bone marrow transplantation.ResultsRPTPα was enriched in the RA synovial lining. RPTPα knockdown impaired RA FLS survival, spreading, migration, invasiveness, and responsiveness to platelet‐derived growth factor, tumor necrosis factor, and interleukin‐1 stimulation. These phenotypes correlated with increased phosphorylation of Src on inhibitory Y527 and decreased phosphorylation of FAK on stimulatory Y397. Treatment of RA FLS with an inhibitor of FAK phenocopied the knockdown of RPTPα. RPTPα‐KO mice were protected from arthritis development, which was due to radioresistant cells.ConclusionBy regulating the phosphorylation of Src and FAK, RPTPα mediates proinflammatory and proinvasive signaling in RA FLS, correlating with the promotion of disease in an FLS‐dependent model of RA.
An Arthritis‐Suppressive and Treg Cell–Inducing CD4+ T Cell Epitope Is Functional in the Context of HLA‐Restricted T Cell Responses
Authors: de Wolf Charlotte , van der Zee Ruurd , den Braber Ineke , Glant Tibor , Maillère Bernard , Favry Emmanuel , van Lummel Menno , Koning Frits , Hoek Aad , Ludwig Irene , van Eden Willem , Broere Femke 
Published: September 2015

ObjectiveWe previously showed that mycobacterial Hsp70‐derived peptide B29 induced B29‐specific Treg cells that suppressed experimental arthritis in mice via cross‐recognition of their mammalian Hsp70 homologs. The aim of the current study was to characterize B29 binding and specific CD4+ T cell responses in the context of human major histocompatibility complex (MHC) molecules.MethodsCompetitive binding assays were performed to examine binding of peptide B29 and its mammalian homologs to HLA molecules. The effect of B29 immunization in HLA–DQ8–transgenic mice with proteoglycan‐induced arthritis was assessed, followed by ex vivo restimulation with B29 to examine the T cell response. Human peripheral blood mononuclear cells were used to investigate the presence of B29‐specific T cells with immunoregulatory potential.ResultsThe binding affinity of the B29 peptide was high to moderate for multiple HLA–DR and HLA–DQ molecules, including those highly associated with rheumatoid arthritis. This binding was considered to be functional, because B29 immunization resulted in the suppression of arthritis and T cell responses in HLA–DQ8–transgenic mice. In humans, we demonstrated the presence and expansion of B29‐specific CD4+ T cells, which were cross‐reactive with the mammalian homologs. Using HLA–DR4+ tetramers specific for B29 or the mammalian homolog mB29b, we showed expansion of cross‐reactive T cells, especially the human FoxP3+ CD4+CD25+ T cell population, after in vitro stimulation with B29.ConclusionThese results demonstrated a conserved fine specificity and functionality of B29‐induced Treg cell responses in the context of the human MHC. Based on these findings, a path for translation of the experimental findings for B29 into a clinical immunomodulatory therapeutic approach is within reach.
Integrated economic models to support decisions on water pricing in biofuel production river basins: three case studies from Brazil
Authors: de Moraes Marcia M. G. A. , Ribeiro Márcia M. R. , Watkins David W. , Viana Jorge H. N. , Figueiredo Luiz E. N. , da Silva Gerald S. , Carneiro Ana C. G. 
Published: September 2015

AbstractEnergy securities, increases in fossil fuel prices, and concern about climate change have been major causes of increased bioenergy production. Because biomass production is rural‐based and generally labor‐intensive, bioenergy development happens mainly in less developed countries. Comprehensive analysis is needed to develop appropriate policies to ensure the sustainability of resource use while promoting economic development. Given this, public policies must promote more efficient inter‐ and intra‐sector water allocation schemes. Economic instruments, such as water pricing and water markets, can be used to avoid transferring negative environmental and economic impacts of meeting global renewable energy targets to production regions. In Brazil, water markets are not allowed, but the law has established raw water charges as a management instrument at the river basin level. However, the majority of bulk water charge systems have been defined exclusively for raising revenue. Integrated economic modeling can be a useful tool to support the design of effective economic instruments. This paper describes the main features of two economic modeling approaches that can support effective water pricing decisions. Three case studies illustrate sugarcane ethanol production river basins in Brazil where water prices have already been established but fail to reflect the real scarcity value of water in the region. In addition, preliminary results of a national‐level economic model being built for Brazil show that increasing water charges for the agricultural sector can have significant effects on water demand and agriculture production. The potential benefits of water pricing policy decisions based on economic modeling results are assessed. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd
Self‐regulation and metacognition in young children: Does it matter if adults are present or not?
Authors: Robson Sue 
Published: September 2015

This paper brings together two areas of considerable interest to researchers, practitioners and policy makers: young children's developing self‐regulation and metacognition, and the impact of adult (practitioner) presence or absence on their behaviour and learning. One hundred and twenty‐eight observations of 29 children aged 4–5 years in a reception class were analysed using a behavioural coding scheme. While the data show that adult presence and absence were both supportive, children were often significantly more likely to show evidence of self‐regulation and metacognition when adults were absent. When present, adults were a focus for the children's attention, and children appeared to cede some responsibility to them, more frequently looking to adults to set goals, allocate roles, check and correct progress and resolve disputes. At the same time, adults played a crucial role in supporting children's procedural knowledge when they were present. Children were keen to display their knowledge to these significant people and often chose to direct their comments to adults. It is suggested that the question of how adults can supportively become involved in young children's activity deserves closer attention, particularly in the context of the development and display of young children's self‐regulation and metacognition.
Facile Immobilization of a Lewis Acid Polyoxometalate onto Layered Double Hydroxides for Highly Efficient N‐Oxidation of Pyridine‐Based Derivatives and Denitrogenation
Authors: Liu Kai , Yao Zhixiao , Miras Haralampos N. , Song Yu‐Fei 
Published: September 2015

AbstractN‐Oxides are a class of highly important compounds that are used widely as synthetic intermediates. In this paper, we demonstrate for the first time the use of a polyoxometalate‐based composite material as a highly efficient heterogeneous catalyst for the N‐oxidation of pyridines and its derivatives in the presence of H2O2 at room temperature. The composite was prepared by the intercalation of the [La(PW11O39)2]11− anion into a layered double hydroxide (LDH) modified with tris(hydroxymethyl)aminomethane (Tris). Additionally, the Tris‐LDH‐La(PW11)2‐based catalyst has been employed for the denitrogenation of a model oil mixture in the presence of 1‐butyl‐3‐methylimidazolium tetrafluoroborate and H2O2. Denitrogenation can be achieved in 40 min at 75 °C. Finally, the heterogeneous catalyst can be recovered easily and reused at least 10 times without a measurable decrease of the catalytic activity and disintegration of the Tris‐LDH‐La(PW11)2 structure.
Template Design and Economical Strategy for the Synthesis of SSZ‐13 (CHA‐Type) Zeolite as an Excellent Catalyst for the Selective Catalytic Reduction of NOx by Ammonia
Authors: Xu Ruinian , Zhang Runduo , Liu Ning , Chen Biaohua , Zhang Qiao Shi 
Published: September 2015

AbstractSSZ‐13 zeolite, a CHA‐type aluminosilicate, has attracted wide attention recently because of its extraordinary physiochemical properties and excellent potential for applications in catalysis. In this work, SSZ‐13 zeolite was synthesized using choline chloride as a CHA‐topology construction template. This new template has a low cost and is environmentally friendly. Specifically it has the appropriate geometry and functional groups for easy self‐assembly as dual molecules to form the CHA topology, which was verified by self‐prepared organic templates with similar structure and functional groups as well as IR spectroscopy and DFT and potential energy distribution calculations. After ion exchange by Cu cations, the as‐synthesized SSZ‐13 exhibited an outstanding performance for the selective catalytic reduction of NO and showed ≈100 % NO conversions across a wide temperature window of 150–450 °C.
A Solid Phase Vibrational Circular Dichroism Study of Polypeptide–Surfactant Interaction
Authors: Novotná Pavlína , Urbanová Marie 
Published: September 2015

AbstractWe studied the interaction of poly‐l‐lysine (PLL) and poly‐l‐arginine (PLAG) with sodium dodecyl sulfate (SDS) surfactant and the interaction of poly‐l‐glutamic acid (PLGA) and poly‐l‐aspartic acid (PLAA) with tetradecyltrimethylammonium bromide (TTAB) surfactant using vibrational circular dichroism (VCD) spectroscopy in the region of C‐H stretching vibration and in the Amide I region both in solution and in mulls. A chirality transfer from polypeptides to achiral surfactants was observed in the C‐H stretching region, where measurements in solution were impossible. This observation was enabled by a special sample treatment technique using lyophilization and the preparation of mulls. This technique demonstrated itself as an interesting and beneficial tool for VCD measurements. In addition, we observed that SDS changed the secondary structure of PLL to the β‐sheet and of PLAG to the α‐helix. TTAB disrupted the PLGA and PLAA structure. These results were obtained in the mull but were confirmed by the VCD spectra measured in solution and by electronic circular dichroism. The chirality transfer from the polypeptides to SDS was caused by polypeptides ordered into a specific conformation during the interaction, while in the TTBA system it was induced primarily by the chirality of the amino acid residues. Chirality 27:965–972, 2015. © 2015 Wiley Periodicals, Inc.
The Effect of Remote Ischemic Conditioning and Glyceryl Trinitrate on Perioperative Myocardial Injury in Cardiac Bypass Surgery Patients: Rationale and Design of the ERIC‐GTN Study
Authors: Hamarneh Ashraf , Sivaraman Vivek , Bulluck Heerajnarain , Shanahan Hilary , Kyle Bonnie , Ramlall Manish , Chung Robin , Jarvis Claire , Xenou Maria , Ariti Cono , Cordery Roger , Yellon Derek M. , Hausenloy Derek J. 
Published: September 2015

ABSTRACTRemote ischemic conditioning (RIC) using transient limb ischemia/reperfusion has been reported to reduce perioperative myocardial injury in patients undergoing coronary artery bypass grafting and/or valve surgery. The role of intravenous glyceryl trinitrate (GTN) therapy administered during cardiac surgery as a cardioprotective agent and whether it interferes with RIC cardioprotection is not clear and is investigated in the ERIC‐GTN trial (
http://www.clinicaltrials.gov: NCT01864252). The ERIC‐GTN trial is a single‐site, double‐blind, randomized, placebo‐controlled study. Consenting adult patients (age > 18 years) undergoing elective coronary artery bypass grafting ± valve surgery with blood cardioplegia will be eligible for inclusion. Two hundred sixty patients will be randomized to 1 of 4 treatment groups following anesthetic induction: (1) RIC alone, a RIC protocol comprising three 5‐minute cycles of simultaneous upper‐arm and thigh cuff inflation/deflation followed by an intravenous (IV) placebo infusion; (2) GTN alone, a simulated sham RIC protocol followed by an IV GTN infusion; (3) RIC + GTN, a RIC protocol followed by an IV GTN infusion; and (4) neither RIC nor GTN, a sham RIC protocol followed by IV placebo infusion. The primary endpoint will be perioperative myocardial injury as quantified by the 72‐hour area‐under‐the‐curve serum high‐sensitivity troponin T. The ERIC‐GTN trial will determine whether intraoperative GTN therapy is cardioprotective during cardiac surgery and whether it affects RIC cardioprotection.
6‐Methyluracil Derivatives as Bifunctional Acetylcholinesterase Inhibitors for the Treatment of Alzheimer's Disease
Authors: Semenov Vyacheslav E. , Zueva Irina V. , Mukhamedyarov Marat A. , Lushchekina Sofya V. , Kharlamova Alexandra D. , Petukhova Elena O. , Mikhailov Anatoly S. , Podyachev Sergey N. , Saifina Lilya F. , Petrov Konstantin A. , Minnekhanova Oksana A. , Zobov Vladimir V. , Nikolsky Evgeny E. , Masson Patrick , Reznik Vladimir S. 
Published: September 2015

AbstractNovel 6‐methyluracil derivatives with ω‐(substituted benzylethylamino)alkyl chains at the nitrogen atoms of the pyrimidine ring were designed and synthesized. The numbers of methylene groups in the alkyl chains were varied along with the electron‐withdrawing substituents on the benzyl rings. The compounds are mixed‐type reversible inhibitors of cholinesterases, and some of them show remarkable selectivity for human acetylcholinesterase (hAChE), with inhibitory potency in the nanomolar range, more than 10 000‐fold higher than that for human butyrylcholinesterase (hBuChE). Molecular modeling studies indicate that these compounds are bifunctional AChE inhibitors, spanning the enzyme active site gorge and binding to its peripheral anionic site (PAS). In vivo experiments show that the 6‐methyluracil derivatives are able to penetrate the blood–brain barrier (BBB), inhibiting brain‐tissue AChE. The most potent AChE inhibitor, 3 d (1,3‐bis[5‐(o‐nitrobenzylethylamino)pentyl]‐6‐methyluracil), was found to improve working memory in scopolamine and transgenic APP/PS1 murine models of Alzheimer's disease, and to significantly decrease the number and area of β‐amyloid peptide plaques in the brain.
The Angelina effect revisited: Exploring a media‐related impact on public awareness
Authors: Lebo Patricia Beatrice , Quehenberger Franz , Kamolz Lars‐Peter , Lumenta David Benjamin 
Published: September 2015

BACKGROUNDIn 2013, Angelina Jolie's double mastectomy and publication of her personal treatment choice for BRCA1 positivity generated considerable media attention. To the authors’ knowledge, the current study is the first prospective survey conducted among the general public to measure a quantifiable media‐related effect on public awareness.METHODSThe authors analyzed the changes in the general public's awareness of reconstructive options in breast cancer among 2 female population‐matched cohorts aged 18 to 65 years (1000 participants in each cohort) before (March 2013; poll 1) and after (June 2013; poll 2) the announcement of Ms. Jolie's mastectomy in May 2013.RESULTSThere was an observed increase in public awareness: significantly more women from poll 2 were aware of reconstructive breast surgery being possible after breast cancer‐related mastectomy, notably with regard to autologous tissue and single‐stage reconstructions. Approximately 20% of the women in poll 2 (205 women) indicated that media coverage regarding Ms. Jolie affected their interest in breast cancer. A question that was exclusive to poll 2 revealed a preference for autologous (66.2%) versus implant‐based (8.2%) reconstructions, with the remainder indicating no preference (25.6%). None of the stratification variables were found to be associated with the above findings.CONCLUSIONSTo the best of the authors’ knowledge, this is the first prospective study to demonstrate a statistically significant impact of a celebrity announcement on public awareness regarding breast cancer treatment. The results underscore the importance of a media‐related impact for professionals in the health care sector, which can serve as a tipping point for raising awareness and improving knowledge concerning a specific disease among the general public. Cancer 2015;121:3959–3964. © 2015 American Cancer Society.
Comparing symptomatic and functional outcomes over 5 years in two nonclinical cohorts characterized by binge eating with and without objectively large episodes
Authors: Palavras Marly A. , Hay Phillipa J. , Lujic Sanja , Claudino Angélica M. 
Published: September 2015

ABSTRACTObjectiveThe aim was to compare symptomatic and functional outcomes over 5 years in women with regular subjective (SBEs), objective (OBEs), and no regular binge eating episodes.MethodData were derived from two cohorts of 330 women with high levels of eating disorders symptoms followed over 5 years. Three groups were formed: (a) regular SBEs but no regular OBEs (N = 68), (b) regular OBEs with or without regular SBEs (N = 154), and (c) with no regular binge eating episodes (N = 108).ResultsAt baseline, the groups did not differ significantly in restraint scores and quality of life. People in the OBE group scored higher than those in the SBE group in body mass index (BMI). Those who had no regular binge eating had lower global Eating Disorder Examination Questionnaire (EDE‐Q) and weight and shape concern scores than those with regular SBEs, and lower eating concern scores than either binge eating groups. Across the follow‐up, there were no significant effects of being in either binge eating or the nonbinge eating group on the rates of change in BMI, general psychological distress, quality of life, or EDE‐Q scores with the exception that OBE group had a significantly different rate of change in eating concern and psychological distress compared to the group without regular binge eating.DiscussionIndividuals that report regular SBEs without regular OBEs represent a group with similar mental hardship and outcomes to those with regular OBEs. The findings support inclusion of regular SBEs in criteria for eating disorder diagnostic categories characterized by recurrent binge eating. © 2015 Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:1158–1165).
Solid lipid microparticles of hardfats produced by spray cooling as promising crystallization modifiers in lipid systems
Authors: Lopes Julice Dutra , Grosso Carlos Raimundo Ferreira , de Andrade Calligaris Guilherme , Cardoso Lisandro Pavie , Basso Rodrigo Corrêa , Ribeiro Ana Paula Badan , Efraim Priscilla 
Published: September 2015

Spray cooling (SC) is a simple and quick technique without the use of solvents that can be applied for the production of microparticles of different materials with diameters in the micrometer range. In this work, the SC technique was evaluated to obtain solid lipid microparticles (SLMs) of fully hydrogenated vegetable oils (hardfats) of soybean, cottonseed, palm, and crambe. SLMs were produced using a laboratory‐scale SC, varying the temperature of the hardfat (70–85°C), SLM solidification temperature (0 and 24°C), nozzle diameter (0.7–1 mm), and air pressure (1.25–1.75 kgf/cm2). The melting point and preferential polymorphisms of the soybean, cottonseed, palm, and crambe hardfats were, respectively, 69.5°C (β), 63.2°C (β′), 60.4°C (β′), and 73.8°C (β′ + β). The analysis of the morphology and particle size distribution showed that all SLMs had a spherical shape, with mean diameters ranging from 60.15 to 2013.99 µm (soybean), 101.25 to 302.85 µm (cottonseed), 56.12 to 2514.32 µm (palm), and 66.10 to 877.93 µm (crambe), depending on the process conditions. It was verified that the atomization process changed the polymorphic habit of all hardfats to the α form. These results indicate that the SC technique may be applied to obtain SLMs of hardfats.Practical applications: Solid lipid microparticles, which are composed of fully hydrogenated vegetable oils and obtained by the spray cooling technique, have the potential to modulate or optimize conventional fat crystallization processes for several different industrial purposes.Solid lipid microparticles of fully hydrogenated crambe oil, obtained by spray cooling technique, under the conditions of Test 9 (T = 80°C; P = 1.50 kgf/cm2; A = 0.7 mm).
Lifelong diet including common unsaturated fatty acids extends the lifespan and affects oxidation in Caenorhabditis elegans consistently with hormesis model
Authors: Fang Bing , Zhang Ming , Ren Fa Zheng , Zhou Xiao Dan 
Published: September 2015

Diets abundant in unsaturated fatty acids (UFAs) help to support a longer and healthier life. We evaluated effects of dietary oleic acid (OA), linoleic acid (LnA), and linolenic acid (ALA) on lifespan and physical activities of Caenorhabditis elegans (C. elegans). Supplementation at doses per plate of 0.5 mg OA, 0.1 mg LnA, or 0.5 mg ALA extended the lifespan of C. elegans by 10.49, 14.17, or 8.47%, respectively (p < 0.05). At these doses, LnA and ALA significantly inhibited growth, pharyngeal pumping, reproduction, and respiration (p < 0.05), while OA did not influence these physiological activities (p > 0.05). Furthermore, OA significantly increased superoxide dismutase, catalase, and glutathione peroxidase activities. Based on results obtained with wild‐type N2, eat‐2, and sod‐2‐mutated C. elegans, OA extended C. elegans lifespan through a hormesis mechanism by activating antioxidant enzymes. In contrast, LnA and ALA acted via a caloric restriction mechanism. Consistent with calorie restriction being a type of stress, however, lifespan was shortened as LnA and ALA doses were further increased. Therefore, the effects of all three fatty acids might be regarded as being consistent with a classic hormesis effect.Practical applications: Oleic acid, linoleic acid, and linolenic acid are the three most common unsaturated fatty acids in vegetable oils. The beneficial effects of oleic acid, linoleic acid, and linolenic acid supplementation on lifespan, normal physiological activities, and oxidation in our study support consumers choosing vegetable oils in their diets.Supplementation with 0.5 mg OA, 0.1 mg LnA or 0.5 mg ALA extended the lifespan of C. elegans by 10.49%, 14.17% or 8.47%, respectively (p < 0.05). At these doses, LnA and ALA significantly inhibited growth, pharyngeal pumping, reproduction and respiration, while OA did not influence these physiological activities and significantly increased superoxide dismutase, catalase and glutathione peroxidase activities. Using the eat‐2, and sod‐2 mutated strains, the anti‐aging mechanism of OA was hormesis, while LnA and ALA acted via caloric restriction.