bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide identification and expression profile analysis of the NF-Y transcription factor gene family in Eucalyptus grandis
Authors: Li Juan , Gong Chaoyan , Zhuang Li , Li Guangyou , Xu Jianmin , Lu Zhaohua 
Published: July 2024

Abstract

Background
The NF-Y (NUCLEAR FACTOR-Y) transcription factor in plants is composed of NF-YA, NF-YB and NF-YC subunits. It is known to play an important role in plant growth and development and response to stress. Although the NF-Y gene family has been systematically studied in many species, the understanding of the NF-Y gene family in Eucalyptus remains unknown.
Results
In this study, 31 (7 EgrNF-YA, 16 EgrNF-YB and 8 EgrNF-YC) EgrNF-Y genes were identified in E. grandis using Arabidopsis NF-Y protein sequences as queries and their structural characteristics were comprehensively analyzed. Phylogenetic, conserved domain and exon-intron structure analyzed that the closer relationship in each subfamily. Multiple alignments showed that all EgrNF-Y proteins had conserved core regions. Chromosomal localization of these genes revealed that they were randomly distributed across 11 chromosomes. Cis-element analysis of promoter indicated that EgrNF-Y gene was affected by various hormonal and abiotic stresses. Furthermore, tissue-specific expression showed that all 30 EgrNF-Y genes were widely expressed in various tissues and organs. Additionally, the stress response pattern of EgrNF-Ys was identified under phosphate-starved, and 12 genes and 3 genes were upregulated more than 2-fold in the leaves and roots, respectively.
Conclusion
Our studies have provided a general understanding of the conservation and characteristics of the EgrNF-Y genes family in E. grandis. And it has been demonstrated that members of the EgrNF-YB1 and EgrNF-YB11 may play important roles in the regulation of floweringin of E. grandis. To provide reference for further study on the role of NF-Y gene in the regulation of flowering in E. grandis. In addition, our also established a theoretical basis for further functional studies on this family.
The Analysis of Switching Losses of IGBT with RPWM Control
Authors: Yi Caihua , Ma Jiaqing , Wu Gangqin , Chen Changsheng , He Zhiqin , Wu Qinmu , Liu Hongju , Li Yongjie 
Published: July 2024

Abstract

When the random pulse width modulation (RPWM) technique is applied to insulated gate bipolar transistor (IGBT) modules, the switching losses are decreased to different degrees. In order to study the principle of this control method, this paper starts from the perspective of Joule heating principle. According to the energy losses and heating characteristics of IGBT module during on-state and off-state processes, the effects of different random pulse width modulation (RPWM) techniques on the switching losses of IGBT module are analyzed and the corresponding theoretical models are established. Experimenting in a three-phase permanent magnet synchronous motor (PMSM) environment, in order to compare the switching Joule losses incurred by the IGBT module with different modulation techniques, four modulation techniques such as random carrier frequency PWM, random duty cycle PWM, random dead zone PWM and non-random PWM modulation techniques are adopted. In addition, the effectiveness of random PWM techniques in reducing switching losses are further verified by comparing the experimental results with different controllers. These research results provide important theoretical guidance for the design and application of power electronic devices, and offer new ideas and methods for improving system performance and reducing energy losses.
Genetic variation, trait association and heritability of root traits in parental and hybrid Brassica napus genotypes under PEG-treated hydroponic culture
Authors: Azim Jaber Bin , Hassan Lutful , Robin Arif Hasan Khan 
Published: July 2024

Abstract

Background: Rapeseed (Brassica napusL.) is an important oilseed crop susceptible to drought stress. Research on rapeseed root systems is limited. This study aimed to determine the effects of Polyethylene Glycol (PEG)-induced osmotic stress in selected rapeseed breeding lines and hybrids, particularly on the root morphology.
Methodology: Plants were cultured hydroponically for 25 days and subjected to two PEG concentrations: 0 (control) and 5%. Two weeks later, nine root traits, five shoot traits, and four biochemical traits were measured after a destructive harvest.
Results: PEG-induced osmotic stress enhanced root dry weight by producing long and thick main and lateral roots and longer root hairs while suppressing the morphological growth of shoots. A negative association among root and shoot traits was found from principal component analysis. PEG treatment also altered the synthesis of antioxidants and reactive oxygen species. There was a trait-specific genotypic response to the two PEG levels. Among the root traits, lateral root length, root hair length, and root dry weight accounted for high variability and heritability.
Conclusion: Root traits showing high heritability can be selected for advancing generations to select rapeseed genotypes with osmotic stress-tolerant root systems. The results imply that rapeseed breeders may consider lateral root traits in drought-stress breeding.
Intraspecific variation in the alkaloids of Adalia decempunctata (Coleoptera, Coccinellidae): sex, reproduction and colour pattern polymorphism.
Authors: ASLAM MUHAMMAD , Nedvěd Oldřich , SLOGGETT JOHN J. 
Published: July 2024

Abstract

In this paper, we examine intraspecific variation in the quantity of alkaloid chemical defence in field collected individuals of the polymorphic ladybird beetle Adalia decempunctata (10spot ladybird). Like its more widely studied relative Adalia bipunctata (2-spot ladybird), A. decempunctata possesses the alkaloids adaline and adalinine, which are, respectively, the major alkaloids of A. bipunctata. We focused especially on alkaloid concentration in relation to colour pattern morph, sex, and the relationship between female and egg parameters. There was a marked sexual dimorphism in the balance of the two alkaloids, with adaline predominating in females and adalinine predominating in males: in males, on average, over 70% of total alkaloid was adalinine. Females had a lower proportion of adalinine (< 10%) than their eggs (> 15%) and relationships between egg alkaloid and female alkaloid or fecundity were weak or non-existent. Colour pattern morph had a borderline significant relationship with adaline concentration and total alkaloid concentration, which could be further explored with laboratory reared individuals. The sexual dimorphism in alkaloid content, which seems likely due to differences in synthesis, might be related to their relative costs to the two sexes and might provide insight into the evolution of alkaloid diversity in ladybirds.
Complete biosynthetic pathway of furochromones and its evolutionary mechanism in Apiaceae plants
Authors: Ye Min , Zou Jianlin , Li Hongye , Nie Bao , Wang Zi-Long , Zhao Chunxue , Tian Yungang , Lin Liqun , Xu Weizhe , Hou Zhuangwei , Sun Wenkai , Han Xiaoxu , Zhang Meng , Wang Hao-Tian , Li Qingyan , Wang Li 
Published: July 2024

Abstract

Furochromones are bioactive and specific secondary metabolites of many Apiaceae plants. Their biosynthesis remains largely unexplored. In this work, we dissected the complete biosynthetic pathway of major furochromones in the medicinal plant Saposhnikovia divaricata by characterizing novel prenyltransferase, peucenin cyclase, methyltransferase, hydroxylase, and glycosyltransferases. De novo biosynthesis of prim-O-glucosylcimifugin and 5-O-methylvisamminoside was then realized in tobacco leaves. Through comparative genomic and transcriptomic analyses, we further found that proximal duplication and high expression of a pentaketide chromone synthase gene SdPCS, together with the presence of a lineage-specific peucenin cyclase gene SdPC, led to the predominant accumulation of furochromones in the roots of S. divaricata among surveyed Apiaceae plants. This study paves the way for metabolic engineering production of furochromones, and sheds light into evolutionary mechanism of furochromone biosynthesis among Apiaceae plants.
Bacteriological quality and safety of handwashing water among food service establishments in Gondar Town, Northwest Ethiopia
Authors: Melkamu Misganaw , Sitotaw Baye , Tadesse Beselam 
Published: July 2024

ABSTRACTUnsafe handwashing water (HWW) has been one of the public health concerns in developing nations like Ethiopia. This study aimed to assess the bacteriological quality and safety of HWW and associated risk factors in food service establishments (FEs) (hotels, restaurants, and cafes) in Gondar Town, Northwest Ethiopia. A cross-sectional study was conducted from February to August 2023. Total coliforms (TC) and fecal coliforms (FC) were enumerated using the membrane filtration method. In addition, potentially pathogenic bacteria were isolated from samples that tested positive for coliforms. The antibacterial susceptibility profile of the isolated bacteria was also determined using the Kirby–Bauer disk diffusion method. Based on the results, 87.2% (for TC) and 52.9% (for FC) of the HWW did not meet water quality standards. The presence of unlicensed managers, untrained food handlers, and poor levels of knowledge and practices on hygiene among HWW were found to be associated risk factors for FC contamination. All the isolated bacteria were resistant to amoxicillin and tetracycline, but susceptible to ciprofloxacin. Moreover, 66–77% of the isolates were multi-drug resistant. Thus, the HWW in FEs of Gondar Town is unsafe for human health, and appropriate measures are needed to control the risks.
Study of the Anticonvulsant Activity of the Simultaneous Administration of Melatonin, Agmatine and Vitamin D3 on Pentylenetetrazole-Induced Seizures in Mice
Authors: Naeini Ali Jamshidi , Mehrzadi Saeed , Azimirad Fahime , Hosseinzadeh Azam 
Published: July 2024

Background:
Epilepsy is a neurological disorder characterized by a decreased level of
vitamins and endogenous antioxidants and an enhanced level of oxidative stress.

Objective:
We designed this study to define the effects of combining melatonin, vitamin D3 (Vit
D3), and agmatine on seizures caused by pentylenetetrazole (PTZ) in male NMRI mice.

Methods:
The experimental groups were as follows: the first group was administered normal saline
(0.5 ml, i.p.) on the last day of the trial, and the second group was administered intraperitoneal
PTZ (60 mg/kg) on that day. Pre-treatment with diazepam (4 mg/kg), vitamin D3 (6000
IU/kg, p.o.), agmatine (40 mg/kg, p.o.), and melatonin (20 mg/kg, p.o) was given to the next four
groups before injecting PTZ (60 mg/kg, i.p.). The last group received a compound therapy of vitamin
D3, melatonin, and agmatine before PTZ injection. Afterward, the latency, duration, and
oxidative stress indications were evaluated in the brains of mice.

Results:
Treatments enhanced the latency of seizure and reduced seizure duration in comparison
with the PTZ group; the compound group increased seizure latency more than Vit D3, agmatine,
and melatonin groups and decreased the duration more than pre-treatment with Vit D3. We observed
enhancement in superoxide dismutase (SOD) and catalase (CAT) activity in the treatment
groups, while in the combination group, elevating CAT activity was comparable with Vit D3 and
agmatine groups. The malondialdehyde level decreased in diazepam, agmatine, melatonin, and
combination groups.

Conclusion:
The compound treatment was more efficient than each one alone in improving PTZinduced
seizure, which may result from suppressing oxidative stress.
Long-term Remission in Functioning Pituitary Adenomas after Medical
Therapy Withdrawal: A Chance for Cushing’s Disease
Authors: Mondin Alessandro , Ceccato Filippo , Scaroni Carla , Barbot Mattia 
Published: July 2024

Background:
The possibility of sustained disease remission in functioning pituitary adenomas
after drug withdrawal is well-known for prolactinomas and it has also been described in a
subset of acromegalic patients. Similarly, medical treatment for Cushing’s Disease (CD) is generally
considered a life-long measure except for previously radio-treated patients. Sparse evidence
of spontaneous remissions in CD has been reported, mainly related to a possible pituitary tumor
apoplexy. To the best of our knowledge, none of these cases has included the use of a pituitary targeting
agent.

Case Presentation:
Herein, we have reported the case of a radiotherapy-naïve patient with persistent
CD after pituitary surgery who participated in the CSOMG230 trials, presenting sustained remission
after Long-acting Release (LAR) pasireotide withdrawal. We have also briefly reviewed
previous cases of sustained remission after somatostatin analogues withdrawal in other functioning
pituitary adenomas. Under monthly pasireotide LAR 40 mg, the patient achieved urinary hormone
control and clinical signs of cortisol excess normalization. After 8 years of treatment, the patient
completed the study protocol and had to withdraw the drug as it was no longer available for
CD in Italy. Before starting new therapies, we reassessed hormone levels that were surprisingly
within normal ranges. At 24 months after the last dose of pasireotide, the patient was still in clinical
and biochemical full remission.

Conclusion:
Far from the general rule, this case suggests that prolonged treatment with
pasireotide LAR might induce a durable CD remission. A dose down-titration/suspension might
be considered in patients well-controlled on long-term therapy and with negative pituitary imaging.
However, close monitoring is recommended given the high rate of complications in untreated
patients.
Assessing the Effectiveness of eHealth Interventions to Manage Multiple Lifestyle Risk Behaviors Among Older Adults: Systematic Review and Meta-Analysis
Authors: Shi Beibei , Li Guangkai , Wu Shuang , Ge Hongli , Zhang Xianliang , Chen Si , Pan Yang , He Qiang 
Published: July 2024

Background
Developing adverse lifestyle behaviors increases the risk of a variety of chronic age-related diseases, including cardiovascular disease, obesity, and Alzheimer disease. There is limited evidence regarding the effectiveness of eHealth-based multiple health behavior change (MHBC) interventions to manage lifestyle risk behaviors.

Objective
The purpose of this systematic evaluation was to assess the effectiveness of eHealth MHBC interventions in changing ≥2 major lifestyle risk behaviors in people aged ≥50 years.

Methods
The literature search was conducted in 6 electronic databases—PubMed, Embase, Web of Science, Scopus, Cochrane Library, and SPORTDiscus—from inception to May 1, 2024. Eligible studies were randomized controlled trials of eHealth interventions targeting ≥2 of 6 behaviors of interest: alcohol use, smoking, diet, physical activity (PA), sedentary behavior, and sleep.

Results
A total of 34 articles with 35 studies were included. eHealth-based MHBC interventions significantly increased smoking cessation rates (odds ratio 2.09, 95% CI 1.62-2.70; P<.001), fruit intake (standardized mean difference [SMD] 0.18, 95% CI 0.04-0.32; P=.01), vegetable intake (SMD 0.17, 95% CI 0.05-0.28; P=.003), self-reported total PA (SMD 0.22, 95% CI 0.02-0.43; P=.03), and objectively measured moderate to vigorous PA (SMD 0.25, 95% CI 0.09-0.41; P=.002); in addition, the interventions decreased fat intake (SMD –0.23, 95% CI –0.33 to –0.13; P<.001). No effects were observed for alcohol use, sedentary behavior, or sleep. A sensitivity analysis was conducted to test the robustness of the pooled results. Moreover, the certainty of evidence was evaluated using the GRADE (Grading of Recommendations Assessment, Development, and Evaluation) framework.

Conclusions
eHealth-based MHBC interventions may be a promising strategy to increase PA, improve diet, and reduce smoking among older adults. However, the effect sizes were small. Further high-quality, older adult–oriented research is needed to develop eHealth interventions that can change multiple behaviors.

Trial Registration
PROSPERO International Prospective Register of Systematic Reviews CRD42023444418; https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023444418
Rank Ordered Design Attributes for Health Care Dashboards Including Artificial Intelligence: Usability Study
Authors: Malkani Melina , Madan Eesha , Malkani Dillon , Madan Arav , Singh Neel , Bamji Tara , Sabharwal Harman 
Published: July 2024

Background
On average, people in the United States visit a doctor 4 times a year, and many of them have chronic illnesses. Because of the increased use of technology, people frequently rely on the internet to access health information and statistics. People use health care information to make better-educated decisions for themselves and others. Health care dashboards should provide pertinent and easily understood data, such as information on timely cancer screenings, so the public can make better-informed decisions. In order to enhance health outcomes, effective dashboards should provide precise data in an accessible and easily digestible manner.

Objective
This study identifies the top 15 attributes of a health care dashboard. The objective of this research is to enhance health care dashboards to benefit the public by making better health care information available for more informed decisions by the public and to improve population-level health care outcomes.

Methods
The authors conducted a survey of health care dashboards with 218 individuals identifying the best practices to consider when creating a public health care dashboard. The data collection was conducted from June 2023 to August 2023. The analyses performed were descriptive statistics, frequencies, and a comparison to a prior study.

Results
From May 2023 to June 2023, we collected 3259 responses in multiple different states around the United States from 218 people aged 18 years or older. The features ranking in descending order of importance are as follows: (1) easy navigation, (2) historical data, (3) simplicity of design, (4) high usability, (5) use of clear descriptions, (6) consistency of data, (7) use of diverse chart types, (8) compliance with the Americans with Disabilities Act, (9) incorporated user feedback, (10) mobile compatibility, (11) comparison data with other entities, (12) storytelling, (13) predictive analytics with artificial intelligence, (14) adjustable thresholds, and (15) charts with tabulated data.

Conclusions
Future studies can extend the research to other types of dashboards such as bioinformatics, financial, and managerial dashboards as well as confirm these top 15 best practices for medical dashboards with further evidentiary support. The medical informatics community may benefit from standardization to improve efficiency and effectiveness as dashboards can communicate vital information to patients worldwide on critically prominent issues. Furthermore, health care professionals should use these best practices to help increase population health care outcomes by informing health care consumers to make better decisions with better data.
Videoconference-Delivered Cognitive Behavioral Therapy for Parents of Adolescents With Internet Addiction: Pilot Randomized Controlled Trial
Authors: Horita Hideki , Seki Yoichi , Yamaguchi Takumi , Shiko Yuki , Kawasaki Yohei , Shimizu Eiji 
Published: July 2024

Background
The rise in internet addiction, including web-based gaming and social networking services, is a serious concern. Even with access to medical institutions and counseling services, individuals with internet addiction, particularly adolescents, often refuse medical treatment or counseling. Parent-focused psychological intervention may lead to positive outcomes by improving the parent-adolescent relationship and helping parents identify and modify their adolescent’s problematic behaviors, including internet addiction.

Objective
This study was a pilot randomized controlled trial to test the feasibility of remote cognitive behavioral therapy via videoconferencing for parents of adolescents with internet addiction.

Methods
A total of 13 parents of adolescents aged 12-20 years with internet addiction were recruited and randomly assigned to either 12 sessions of the videoconference-delivered cognitive behavioral therapy (vCBT) group (n=6, 46%) or the waitlist control group (n=7, 54%). The study period was from March 1, 2018, to March 31, 2022. The primary outcome was the scores of the Young Internet Addiction Test reported by the adolescents. The secondary outcomes were adolescents’ hours of internet use per day (Internet Addiction Test), reported by the adolescents and by their parents; the Young Diagnostic Questionnaire, completed by the parents; and the quality of life of the adolescents and the parents, measured by the EQ-5D-5L. These were evaluated at weeks 0 and 13.

Results
As the primary outcome, the mean total Internet Addiction Test score decreased from 67.7 (SD 18.3; 6/13, 46%) at week 0 to 56.2 (SD 25.1; 5/9, 56%) at week 13 in the vCBT group, compared to an increase from 66.9 (SD 21.9; 7/13, 54%) to 68.0 (SD 18.7; 4/9, 44%) in the control group. For all outcomes, no significant differences were found between the 2 groups (all P>.05).

Conclusions
This study suggested the practical feasibility of vCBT for parents of adolescents with internet addiction. Further large-scale, multicenter randomized controlled trials are necessary to examine the effectiveness.

Trial Registration
UMIN Clinical Trials Registry UMIN000032483; https://tinyurl.com/yuhen6c9
Exercise and Cognitive Function in Chronic Kidney Disease
Authors: Bradshaw Ellen , Alejmi Abdulfattah , Rossetti Gabriella , D'Avossa Giovanni , Macdonald Jamie Hugo 
Published: July 2024

Key Points

Cognitive impairment is common in CKD. Exercise targets multiple risk factors of cognitive decline.Meta-analysis found that exercise had a small but positive effect on cognitive function in CKD, albeit the quality of evidence was low.Further analyses revealed that aerobic exercise was particularly beneficial and that exercise did not substantially increase harms.

Background
People living with CKD are at higher risk of cognitive impairment. Exercise may improve cognitive function. This systematic review and meta-analysis of randomized controlled trials was completed to determine the efficacy and harms of exercise in improving cognitive function in people living with CKD.

Methods
A systematic literature review identified randomized controlled trials of people with any stage of CKD, with an intervention that exercised large-muscle groups, and with a validated outcome measure of cognitive function. First, harms were analyzed. Then a random-effects meta-analysis was completed with subsequent planned subgroup analyses to investigate heterogeneity between CKD stages and treatments; between different exercise types, durations, and intensities; and between different outcome methodologies. Finally, quality of evidence was rated.

Results
Nineteen trials randomized 1160 participants. Harms were reported on 94 occasions in intervention groups versus 83 in control. The primary analysis found that exercise had a small but statistically significant effect on cognition in CKD (effect size=0.22; 95% confidence intervals, 0.00 to 0.44; P = 0.05). However, the quality of evidence was rated as low. Subgroup analyses found that type of exercise moderated the effect on cognition (chi square=7.62; P = 0.02), with positive effects only observed following aerobic exercise (effect size=0.57; 95% confidence interval, 0.21 to 0.93; P = 0.002).

Conclusions
Across the spectrum of CKD, exercise had a small but positive and clinically meaningful effect on cognitive function and did not seem to be harmful. Aerobic exercise was particularly beneficial. However, the results must be interpreted cautiously because of the low quality of evidence. Nevertheless, care teams may choose to recommend aerobic exercise interventions to prevent cognitive decline. Researchers should design unbiased studies to clarify what intensity and duration of exercise is required to maximize efficiency of such exercise interventions.