bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhodamine‐Ethylenediol, A Novel Vital Fluorescent Probe for Labelling Alkaline Phosphatase‐Rich Organelles
Authors: Chen Yau‐Hung , Chiou Chien‐Hui , Chen Wei‐Li , Jhou Yu‐Ru , Lee Ya‐Ting , Cheng Chien‐Chung 
Published: May 2015

AbstractZebrafish have received considerable attention as an organism‐based model in the development of pharmacological agents.1,2Many small molecules applied to zebrafish show important behaviours and may constitute new kinds of markers for clinical purposes.3Analysis of these molecules can facilitate the development of useful tools for monitoring environmental changes.4Many chemicals that are toxic to the environment are known to influence the sensory systems of humans5and fish.6One important sensory system in all fish is the lateral line organ,7which is readily accessible for the assessment of environmental changes.8Neuromasts, which are located on the surface of the fish body, are one of the major components of the lateral lines of the zebrafish.9Copper‐enriched water is known to affect the olfactory system in fish. Therefore, small molecules that induce specific patterns in the neuromasts of zebrafish should provide an important animal model with which to explore the effects of environmental changes on the sensory system.10,11Recently, chemical sensors based on the rhodamine skeleton12have been designed to specifically detect metal ions, such as Cu(II)13and Fe(III)/Hg(II),14in zebrafish. However, there has been no report of these rhodamine derivatives used in the specific recognition of the sensory system of zebrafish. Commonly, the sensory system is studied with antibody staining assays of scarified fish. Here, we report that a new rhodamine derivative can be used as a fluorescent chemical probe to visualize the neuromasts and intestinal villi of living zebrafish. Based on the specific recognition of this area in zebrafish, we narrowed the possible enzymes targeted by this rhodamine probe to alkaline phosphatase and confirmed this with a binding assay. It is a well‐recognized challenge to develop a fluorescent chemical probe that specifically recognizes a particular enzyme. Furthermore, the transfer of phosphate groups to certain enzymes can activate their catalytic reactivity, triggering a cascade reaction in a signal transduction pathway. The alkaline‐phosphatase‐specific recognition by this rhodamine derivative may be applicable to clinical purposes.
Synthesis of Novel Aryloxy Propanoyl Thiadiazoles as Potential Antihypertensive Agents
Authors: Samel Amarish B. , Pai Nandini R. 
Published: May 2015

Abstract2‐Amino‐5‐aryl/alkyl‐1,3,4‐thiadiazoles 3a‐e were synthesized from aliphatic and aromatic acids and thiosemicarbazide. These 2‐amino‐5‐aryl/alkyl‐1,3,4‐thiadiazoles 3a‐e were condensed with 2‐(naphthalenyloxymethyl) oxirane 4a‐b to prepare some naphthalenyloxy‐propanol amine derivatives 5a‐j. These compounds were synthesized as potential antihypertensive agents.
Pharmacometrics‐guided drug development of antihyperhidrosis agents
Authors: Mehrotra Shailly , Schmith Virginia D. , Dumitrescu Teodora Pene , Gobburu Jogarao 
Published: May 2015

AbstractThe objective of the present work was to use modeling and simulation to inform trial design of a proof‐of‐concept study for agents used in the treatment of hyperhidrosis. Data were available from 36 subjects who received the vehicle, 2% or 4% topical glycopyrrolate wipes daily for 4 weeks, with response (hyperhidrosis disease severity scale [HDSS] and sweat production [SP]) measured weekly. The HDSS and SP time courses were best described using a longitudinal model with maximum response achieved by 1 week. Glycopyrrolate 4% had a higher HDSS responder rate than 2% (50% vs 33%) and placebo (0%) at week 1. Mean change from baseline (mg/5 min [SD]) in SP at week 1 was ‐90 (220), ‐185 (214), and ‐271 (265) for placebo, 2%, and 4% glycopyrrolate, respectively. Subjects with higher baseline SP had higher sweat reduction from baseline. Virtual clinical trials were simulated and analyzed using conventional (at the end of the study) versus model‐based methods to determine sample size for achieving 80% power to identify a dose–response relationship. Twenty‐seven subjects compared with at least 120 subjects would be needed using model‐based and conventional methods, respectively. Thus, the model‐based method using longitudinal data required fewer subjects than the conventional single‐point method.
Normal T2 map profile of the entire femoral cartilage using an angle/layer‐dependent approach
Authors: Kaneko Yasuhito , Nozaki Taiki , Yu Hon , Chang Andrew , Kaneshiro Kayleigh , Schwarzkopf Ran , Hara Takeshi , Yoshioka Hiroshi 
Published: April 2015

PurposeTo create standard T2 map profiles from the entire femoral cartilage of healthy volunteers in order to assess regional variations using an angular and layer‐dependent approach.Materials and MethodsTwenty healthy knees were evaluated using 3T sagittal images of a T2 mapping sequence. Manual segmentation of the entire femoral cartilage was performed slice‐by‐slice by two raters using MatLab. Inter‐ and intrarater reliabilities were calculated using intraclass correlation coefficient (ICC) and Bland–Altman analysis. T2 values were analyzed with respect to specific locations (medial condyle, trochlea, and lateral condyle), angles to B0, and layers of cartilage (whole, deep, and superficial).ResultsInter‐ and intrarater reliability obtained from the entire femoral cartilage was excellent (ICC = 0.84, 0.86, respectively). The ICCs around the trochlea were lower than those of the medial and lateral condyle. Both the inter‐ and intrarater Bland–Altman plots indicated larger differences in pixel count are seen as the size of the angular segment becomes larger. T2 values were significantly higher in the superficial layer compared to the deep layer at each femoral compartment (P < 0.001). A magic angle effect was clearly observed, especially within the whole and deep layer over the medial and lateral femoral condyles, except for the superficial layer at the medial condyle.ConclusionThe normal T2 map profiles of the entire femoral cartilage showed variations in ICCs by location and in T2 values by angles and layers. These profiles can be useful for diagnosis of early cartilage degeneration in a specific angle and layer of each condyle and trochlea. J. MAGN. RESON. IMAGING 2015;42:1507–1516.
Framework for the simulation of wireless power transmission over ultra wide band links in multipath propagation environments in the vicinity of the human body
Authors: Cuyckens Thomas , Rogier Hendrik 
Published: May 2015

SummaryIn this contribution, a modular framework for ultra wide band wireless power transmission in proximity of the human body is presented. The goal of this work is to simplify the study of wireless links by considering antenna models, including radiation characteristics when the antenna is deployed on the human body, and the power transferred over them in multipath, shadowed propagation environments, such as powering textile antenna systems completely integrated inside garments. To obtain this goal, antenna links are partitioned in black box models for all elementary building blocks, such as antenna transfer functions, propagation channels, active components and more, that can be recombined as one sees fit. The recombination allows for accurate simulation of intricate systems. For small examples, considerable speed and memory gains were observed (a factor 6.20 in time and 1.75 in memory), while for more elaborate examples, it is even impossible to simulate a reference solution. Copyright © 2015 John Wiley & Sons, Ltd.
Painful Mouth and Moving Tongue: Syndrome or Casual Association?
Authors: Fernández José M. , Ibañez Ane M. , Aranzabal Inés M. , Freijo María del Mar , Septién Miren Karmele 
Published: April 2015

AbstractThis study presents a case series with a clinical syndrome characterized by burning pain in the mouth area and choreic movements of the tongue. This symptom complex is chronic and it predominantly affects elderly women. Pathophysiology of this entity is uncertain. Many of the patients had been treated with dopaminergic blocking agents previously, and therefore it could be a peculiar tardive syndrome. Similarities with other conditions involving pain and abnormal movements affecting the same anatomical area are highlighted.
Manual classification strategies in the ECOD database
Authors: Cheng Hua , Liao Yuxing , Schaeffer R. Dustin , Grishin Nick V. 
Published: April 2015

ABSTRACT ECOD (Evolutionary Classification Of protein Domains) is a comprehensive and up‐to‐date protein structure classification database. The majority of new structures released from the PDB (Protein Data Bank) each week already have close homologs in the ECOD hierarchy and thus can be reliably partitioned into domains and classified by software without manual intervention. However, those proteins that lack confidently detectable homologs require careful analysis by experts. Although many bioinformatics resources rely on expert curation to some degree, specific examples of how this curation occurs and in what cases it is necessary are not always described. Here, we illustrate the manual classification strategy in ECOD by example, focusing on two major issues in protein classification: domain partitioning and the relationship between homology and similarity scores. Most examples show recently released and manually classified PDB structures. We discuss multi‐domain proteins, discordance between sequence and structural similarities, difficulties with assessing homology with scores, and integral membrane proteins homologous to soluble proteins. By timely assimilation of newly available structures into its hierarchy, ECOD strives to provide a most accurate and updated view of the protein structure world as a result of combined computational and expert‐driven analysis. Proteins 2015; 83:1238–1251. © 2015 Wiley Periodicals, Inc.
A review on climate‐model‐based seasonal hydrologic forecasting: physical understanding and system development
Authors: Yuan Xing , Wood Eric F. , Ma Zhuguo 
Published: May 2015

Climate‐model‐based seasonal hydrologic forecasting (CM‐SHF) is an emerging area in recent decade because of the development of coupled atmosphere‐ocean‐land general circulation models (CGCMs) and land surface hydrologic models, and increasing needs for transferring the advances in climate research into hydrologic applications within the framework of climate services. In order to forecast terrestrial hydrology from monthly to seasonal time scales, a CM‐SHF system should take advantage of important information from initial land surface conditions (ICs) as well as skillful seasonal predictions of atmospheric boundary conditions that mostly rely on the predictability of large‐scale climate precursors such as the El Niño Southern Oscillation (ENSO). The progresses in the understanding of seasonal hydrologic predictability in terms of ICs and climate precursors are reviewed, and future emphases are discussed. Both the achievements and challenges of the CM‐SHF system development, including multimodel ensemble prediction, seamless hydrologic forecasting, dynamical downscaling, hydrologic post‐processing, and seasonal forecasting of hydrologic extremes with the hyper‐resolution modeling framework that is able to address both the climate change and water resources management impacts on terrestrial hydrology, are presented. Regardless of great strides in CM‐SHF, a grand challenge is the effective dissemination of the information provided by the seasonal hydrologic forecasting system to the decision‐makers, which cannot be resolved without cross‐disciplinary dialog and collaboration. WIREs Water 2015, 2:523–536. doi: 10.1002/wat2.1088This article is categorized under:

Engineering Water > Planning Water
Science of Water > Water Extremes
High Saturated Fat Diet Alters the Lipid Composition of Triacylglycerol and Polar Lipids in the Femur of Dam and Offspring Rats
Authors: Miotto Paula M. , Castelli Laura M. , Amoye Foyinsola , Ward Wendy E. , LeBlanc Paul J. 
Published: April 2015

AbstractPrevious work has shown that dietary lipids alter femur lipid composition. Specifically, we have shown that exposure to high saturated fatty acid (SFA) diets in utero, during suckling, or post‐weaning alters femur total lipid composition, resulting in higher percent bone mass in males and females and bone mineral density (BMD) in female offspring with no effect on bone mineral outcomes in dams. Comparatively, high n‐3 polyunsaturated fatty acid (PUFA) diets increase femur polar (PL) lipid n‐3 content, which has been associated with increased bone mineral content and strength. However, the extent that PL or triacylglycerol (TAG) lipids change with high SFA diets is unknown. The current investigation examined the influence of a high SFA diet (20 % lard by weight) on femur PL and TAG lipid composition in 5‐month old female Wistar rats (fed high SFA diet from age 28 days onwards; dams) and their 19‐day old offspring (exposed to high SFA in utero and during suckling; pups). High SFA exposure resulted in increased monounsaturates and decreased n‐3 and n‐6 PUFA in the TAG fraction in both dams and pups, and higher SFA and n‐6:n‐3 ratio in dams only. The PL fraction showed decreased n‐6 PUFA in both dams and pups. The magnitude of the diet‐mediated responses, specifically TAG 18:1 and PL n‐6 PUFA, may have contributed to the previously reported altered BMD, which was supported with correlation analysis. Future research should investigate the relationship of diet‐induced changes in bone lipids on bone structure, as quantified through micro‐computed tomography.
Infection Prevention: The Surgical Care Continuum
Authors: Barnes Sue 
Published: May 2015

AbstractLack of careful attention to the increasing complexity of surgical procedures, instrument cleaning and processing, and the transition of surgical and other invasive procedures from the OR to areas outside the traditional hospital OR can contribute to surgical site infection (SSI) risk. Regardless of the location of an intervention, when basic infection prevention measures are applied reliably, even low infection rates can be reduced. To address infection prevention challenges, infection preventionists (IPs) must be well informed regarding infection risk and prevention during surgical and other invasive procedures and the effect a facility type may have on patients’ infection risk. The IP must have a solid understanding of surgical asepsis, instrument disinfection, and sterilization to educate and support clinical teams in identifying opportunities for improvement relative to infection prevention.
Guideline Implementation: Surgical Instrument Cleaning
Authors: Cowperthwaite Liz , Holm Rebecca L. 
Published: May 2015

AbstractCleaning, decontaminating, and handling instructions for instruments vary widely based on the type of instrument and the manufacturer. Processing instruments in accordance with the manufacturer's instructions can help prevent damage and keep devices in good working order. Most importantly, proper cleaning and disinfection may prevent transmission of pathogenic organisms from a contaminated device to a patient or health care worker. The updated AORN “Guideline for cleaning and care of surgical instruments” provides guidance on cleaning, decontaminating, transporting, inspecting, and storing instruments. This article focuses on key points of the guideline to help perioperative personnel implement appropriate instrument care protocols in their practice settings. The key points address timely cleaning and decontamination of instruments after use; appropriate heating, ventilation, and air conditioning parameters for the decontamination area; processing of ophthalmic instruments and laryngoscopes; and precautions to take with instruments used in cases of suspected prion disease. Perioperative RNs should review the complete guideline for additional information and for guidance when writing and updating policies and procedures.
Disks aligned in a turbulent channel
Authors: Voth Greg A. 
Published: April 2015

Anisotropic particles are suspended in a wide range of industrial, environmental and biological fluid flows. The orientations of these particles are sometimes randomized by turbulence, but often they are brought into preferential alignment by the fluid flow. In a recently published study, Challabotla, Zhao & Andersson (J. Fluid Mech., vol. 766, 2015, R2) performed the first numerical simulations of inertial disks in a turbulent channel flow. They find that disks can be made to preferentially align either parallel or perpendicular to the wall depending on the particle density. Particle shape also affects alignment, particularly for lower density particles, and the alignment of disks is quite different from the alignment of fibres.
Unusual ambulacral branching pattern in a new Ordovician giant edrioasteroid,Bizarroglobus
Authors: Sumrall Colin D. , Sprinkle James 
Published: April 2015

AbstractAn unusual, new, giant edrioasteroidBizarroglobus medusaen. gen. n. sp. is described from the Middle Ordovician Kanosh Shale of west-central Utah. This species has a pattern of ambulacral branching with side ambulacra arising alternately from a main ambulacral trunk, previously undocumented in edrioasteroids. This pattern is interpreted as a strategy for allometrically increasing the feeding surface during ontogeny.Bizarroglobusfurther differs from other isorophid edrioasteroids in the plating of the peripheral rim, and the presence of pores in the interambulacral plates primarily along the edges of the ambulacra.
A columnal-bearing eocrinoid from the Cambrian Burgess Shale (British Columbia, Canada)
Authors: Sumrall Colin D. , Zamora Samuel 
Published: April 2015

AbstractA new eocrinoid ?Ubaghsicystissp. from the middle Cambrian (Series 3, Stage 5) Burgess Shale is reported based on a single known specimen. This species extends the stratigraphic range of columnal-bearing eocrinoids in Laurentia significantly from Cambrian Stage 7 (Guzhangian) to Stage 5. It increases the diversity of echinoderms in this well-known fossil-Lagerstätte, provides the oldest evidence of columnal-bearing eocrinoids from Laurentia, and further documents the cosmopolitan distribution of middle Cambrian echinoderm clades.
Variable and invariable liaison in a corpus of spoken French
Authors: MEINSCHAEFER JUDITH , BONIFER SVEN , FRISCH CHRISTINE 
Published: April 2015

ABSTRACTUsing texts selected from the C-Oral-Rom corpus, this study considers how linguistic and sociolinguistic variables affect liaison. In the majority of cases, liaison appears on monosyllabic function words. Individual lexemes differ greatly in rate of liaison. With regard to sociolinguistic variation, female speakers realize liaison consonants more often than male speakers, younger speakers realize it more often than older speakers, and liaison rates for speakers without university degree are higher than for speakers with university degree. Results are discussed in the light of models of prosodic structure and with respect to their implications for models of socio-linguistic variation.
237 Gbit/s unrolled hardware polar decoder
Authors: Giard P. , Sarkis G. , Thibeault C. , Gross W.J. 
Published: May 2015

A new architecture for a polar decoder using a reduced complexity successive‐cancellation (SC) decoding algorithm is presented. This novel fully unrolled, deeply pipelined architecture is capable of achieving a coded throughput of over 237 Gbit/s for a (1024, 512) polar code implemented using a field‐programmable gate array. This decoder is two orders of magnitude faster than state‐of‐the‐art polar decoders.
Compact CPW‐fed triple‐band antenna for diversity applications
Authors: Wu Rui‐Zhi , Wang Peng , Zheng Qiang , Li Rui‐Peng 
Published: May 2015

A compact coplanar waveguide (CPW)‐fed triple‐band monopole antenna with high‐band isolation for various wireless applications is presented. The proposed antenna is composed of a CPW‐fed structure and a hexagon‐shaped patch on which a hexagon‐shaped slot is etched for covering the wireless local area network (WLAN) band (2.4/5.2/5.8 GHz), and occupies a very compact size of 26 × 25 mm2. By introducing a half‐hexagon‐shaped stub, additional bands can be realised covering the worldwide interoperability for microwave access (WIMAX) band (2.5/3.5/5.5 GHz). The proposed antenna has been fabricated and measured; results show a higher isolation between adjacent bands, good radiation pattern characteristics and stable gains in the operating bands. The simple feeding structure, compact size and uniplanar design make it easy to be integrated within portable devices for wireless communication.
Design of Ku‐band transformer‐based cross‐coupled complementary LC‐VCO
Authors: Jalalifar M. , Byun G.‐S. 
Published: May 2015

A transformer‐based cross‐coupled complementary LC voltage‐controlled oscillator (VCO) is investigated which is capable of operating at the Ku‐band frequency range with low power consumption. The VCO simultaneously achieves low phase noise and low power consumption. The VCO demonstrates a measured tuning range of 12.1–13.1 GHz and a phase noise of −116.4 dBc/Hz at 1 MHz at a centre frequency of 12.4 GHz. The VCO consumes 0.96 mW from a 0.45 V supply voltage with a figure of merit of 198.4. It is implemented in a standard 0.13 µm CMOS process with an area of 0.23 mm2.
Genetically encoded sensors of protein hydrodynamics and molecular proximity
Authors: Hoepker Alexander C. , Wang Ariel , Le Marois Alix , Suhling Klaus , Yan Yuling , Marriott Gerard 
Published: May 2015

Significance
The lumazine binding protein (LUMP) emits a cyan-colored fluorescence and has the longest average fluorescence lifetime of any genetically encoded fluorescent protein complex. Coupled with a small mass of 20 kDa, LUMP and its fusion with capture sequences are exploited as unique sensors of protein hydrodynamics and are shown to enable quantitative fluorescence anisotropy imaging of specific target proteins in vitro and in vivo. Moreover, the surface location of the lumazine probe is shown to improve the efficiency of Förster resonance energy transfer (FRET) with the Venus acceptor protein compared with CFP, which is used in the development of a new class of FRET-based sensor.
‘Flatten plus’: a recent implementation in WSxM for biological research
Authors: Gimeno Alejandro , Ares Pablo , Horcas Ignacio , Gil Adriana , Gómez-Rodríguez José M. , Colchero Jaime , Gómez-Herrero Julio 
Published: May 2015

Abstract
Summary: Scanning probe microscopy (SPM) is already a relevant tool in biological research at the nanoscale. We present ‘Flatten plus’, a recent and helpful implementation in the well-known WSxM free software package. ‘Flatten plus’ allows reducing low-frequency noise in SPM images in a semi-automated way preventing the appearance of typical artifacts associated with such filters.
Availability and implementation: WSxM is a free software implemented in C++ supported on MS Windows, but it can also be run under Mac or Linux using emulators such as Wine or Parallels. WSxM can be downloaded from http://www.wsxmsolutions.com/.
Contact: ignacio.horcas@wsxmsolutions.com