bluuebunny/crossref_metadata_2025_split huggingface dataset:
How to assess the quality of your analytical method?
Authors: Topic Elizabeta , Nikolac Nora , Panteghini Mauro , Theodorsson Elvar , Salvagno Gian Luca , Miler Marijana , Simundic Ana-Maria , Infusino Ilenia , Nordin Gunnar , Westgard Sten 
Published: September 2015

AbstractLaboratory medicine is amongst the fastest growing fields in medicine, crucial in diagnosis, support of prevention and in the monitoring of disease for individual patients and for the evaluation of treatment for populations of patients. Therefore, high quality and safety in laboratory testing has a prominent role in high-quality healthcare. Applied knowledge and competencies of professionals in laboratory medicine increases the clinical value of laboratory results by decreasing laboratory errors, increasing appropriate utilization of tests, and increasing cost effectiveness. This collective paper provides insights into how to validate the laboratory assays and assess the quality of methods. It is a synopsis of the lectures at the 15th European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) Continuing Postgraduate Course in Clinical Chemistry and Laboratory Medicine entitled “How to assess the quality of your method?” (Zagreb, Croatia, 24–25 October 2015). The leading topics to be discussed include who, what and when to do in validation/verification of methods, verification of imprecision and bias, verification of reference intervals, verification of qualitative test procedures, verification of blood collection systems, comparability of results among methods and analytical systems, limit of detection, limit of quantification and limit of decision, how to assess the measurement uncertainty, the optimal use of Internal Quality Control and External Quality Assessment data, Six Sigma metrics, performance specifications, as well as biological variation. This article, which continues the annual tradition of collective papers from the EFLM continuing postgraduate courses in clinical chemistry and laboratory medicine, aims to provide further contributions by discussing the quality of laboratory methods and measurements and, at the same time, to offer continuing professional development to the attendees.
Atmospheric circulation and storm events in the Baltic Sea
Authors: Surkova G. V. , Arkhipkin Victor S. , Kislov Alexander V. 
Published: September 2015

Abstract
The storm events in the Baltic Sea are examined in connection with the main weather patterns grouped into the circulation types (CTs), and their changes in present climate. A calendar of storms was derived from results of wave model SWAN (Simulating WAves Nearshore) experiments for 1948-2011. Based on this calendar, a catalogue of atmospheric sea level pressure (SLP) fields was prepared for CTs from the NCEP/NCAR dataset. SLP fields were then analyzed using a pattern recognition algorithm which employed empirical orthogonal decomposition and cluster analysis. For every CT we conducted an analysis of their seasonal and interannual changes, along with their role in storm event formation. An increase of the storm CTs’ frequency in the second part of the 20th century was shown to be in a close agreement with teleconnection circulation patterns such as the Arctic Oscillation, North Atlantic Oscillation and the Scandinavian blocking.
Development of Dynamically Optimized Wireless Transmission System for Wireless Sensor Networks in Highrise Apartment Building Heating Systems / Optimizētas Dinamiskās Bezvadu Sensoru Tīklu Pārraides Risinājuma Izstrāde Daudzstāvu Ēku Apkures Sistēmas Parametru Izgūšanai Un Vadībai
Authors: Isakovs V. , Kondratjevs K. , Kunicina N. , Freliha B. , Zabasta A. 
Published: September 2015

Abstract
Smart meters will be at the centre of the integrated solutions, including interaction with other networks in the next decade. The developed solution for multi-apartment building ventilation and heating parameter monitoring aims to increase energy efficiency and optimal control of the existing system. Dynamic control of heat and ventilation systems, heat loss detection, calculation and mitigation, and individual heat energy accounting are difficult tasks to accomplish. This article deals with the data transmission system using battery powered ISM band radio transmitters. The temperature measurement sensors with cumulative temperature reading are used as sensor part for this solution. The offered approach to monitoring system is used for overall building and individual apartment monitoring. Data coding and antenna designs are explained for this particular application.
Development of Solar Powered Feeding Scheme for Wireless Sensor Networks in low Solar Density Conditions / Bezvadu Sensoru Tīklu Elektroapgādes Sistēmas Izstrāde, Kas Izmanto Saules Paneļus Un Darbojas Pazeminātas Saules Radiācijas Apstākļos
Authors: Kondratjevs K. , Zabasta A. , Selmanovs-Pless V. 
Published: September 2015

Abstract
In the recent years, there has been significant research focus on the safety and reliability of data harvesting and optimal energy consuming by wireless sensor network nodes. If external electrical power fails, the node needs to be able to send notifications to the utility demanding the use of backup energy strategies. The authors of the research offer an approach that can help to use PV panels as an alternative power source for WSN nodes in particular irradiation conditions. Survey and testing of the main types of PV panels offered on the market in conditions closed to real ones, in which WSN nodes are maintained, have been implemented. Based on the test results, maximum power control module parameters can be calculated in order to achieve the best effectiveness of the power control system for a selected type of PV panel or panel group. The novelty of the research is an approach that includes an original test bed design for PV testing, PV testing method and selection of design and MPP control module parameters, which ensure maximum effectiveness of WSN node power feeding.
Imaging of myocardial infarction with thermoacoustic tomography: An ex vivo study
Authors: Weizhi Qi , Wei Cai , Xi Lu , Jian Rong , Lin Huang 
Published: September 2015

AbstractIn the present study, we evaluated the feasibility of thermoacoustic tomography (TAT) for imaging of ex vivo mouse hearts with myocardial infarction. A circular scanning TAT system with an unfocused transducer was used to recover the dielectric property distribution of normal andmyocardial infarcted mouse heart tissues. The applicability of this myocardial infarction imaging system was validated using a model of myocardial infarction in two Sprague-Dawley rats and verified through comparison with magnetic resonance imaging (MRI). TAT results not only indicated the location and ischemia and the extent of myocardial ischemia (MI), but also showed good imaging contrast between infarcted and normal myocardium without the use of contrast agent. The experimental results suggest that TAT may provide a unique opportunity to enable real-time precision imaging to determine the site of injury intraoperatively
Genetic variability of Colobanthus quitensis from King George Island (Antarctica)
Authors: Androsiuk Piotr , Chwedorzewska Katarzyna , Szandar Kamil , Giełwanowska Irena 
Published: September 2015

AbstractAntarctic pearlwort (Colobanthus quitensis) is one of the flowering plant species considered native to maritime Antarctica. Although the species was intensively analyzed towards its morphological, anatomical and physiological adaptation to local environment, its genetic variability is still poorly studied. In the presented study, a recently developed retrotransposon-based DNA marker system (inter Primer Binding Site – iPBS) was applied to assess the genetic diversity and differentiation of C. quitensis populations from King George Island (South Shetland Islands, West Antarctic). A total of 143 scoreable bands were detected using 7 iPBS primers among 122 plant specimens representing 8 populations. 55 (38.5%) bands were found polymorphic, with an average of 14.3% polymorphic fragments per primer. Nine of all observed fragments were represented as a private bands deployed unevenly among populations. Low genetic diversity (on average He= 0.040 and I = 0.061) and moderate population differentiation (FST= 0.164) characterize the analyzed material. Clustering based on PCoA revealed, that the populations located on the edges of the study area diverge from the central populations. The pattern of population differentiation corresponds well with their geographic location and the characteristics of the sampling sites. Due to the character of iPBS markers, the observed genetic variability of populations may be explained by the genome rearrangements caused by mobilization of mobile genetic elements in the response to various stress factors. Additionally, this study demonstrates the usefulness of iPBS markers for genetic diversity studies in wild species.
Proton pump inhibitors: recent developments in analytical methodologies
Authors: Patel Mehul M. , Bhuva Satish D. , Patel Miketa M. 
Published: September 2015

AbstractAn extensive survey of the literature published in various analytical and pharmaceutical chemistry-related journals have been conducted, and the instrumental analytical methods that were developed and used for the determination of proton pump inhibitors in bulk drugs, formulations, and biological fluids have been reviewed. This review covers the time period from 1990 to 2011 during which 80 analytical methods, including all types of spectrophotometric and chromatographic techniques were reported. High-performance liquid chromatography (HPLC) with ultra violet (UV) detection was found to be the technique of choice for many workers, and more than 50 methods were based on liquid chromatography (LC) and ultra violet (UV). A critical analysis of the reported data was carried out and the present state of the art of the analytical techniques for the determination of omeprazole, esomeprazole, pantoprazole, rabeprazole, dexrabeprazole, tenatoprazole, lansoprazole, and dexlansoprazole is discussed.
Assessing Intake of Water and Sugar-Sweetened Beverages in Adolescents: its Relationship with Weight Status
Authors: Mihalache Laura , Niță Otilia , Gherasim Andreea , Ringhilescu Irina , Popa Alina Delia , Graur Mariana , Arhire Lidia Iuliana 
Published: September 2015

Abstract
Background and aims. The aim of the study is to evaluate fluid intake during adolescence and correlate it with weight status. Material and methods. We assessed fluid intake using a validated questionnaire in a group of 106 adolescent students (22 boys - 20.8%), aged 15-19 years. Weight status was evaluated with the BMI-for-age values,using growth normograms. Results. There were no statistically significant differences in the frequency of water intake between sexes (p>0.05). Water intake at least 3 times a day was declared by 72.16% of normal weight students and in 66% of overweight and obese, the difference being statistically significant (p=0.003). Boys consumed larger amounts of water (p=0.042) than girls. Intake of 100% natural fruit juice was significantly higher in boys compared to girls (p=0.002). A significantly higher percentage of normal weight adolescents consumed≤500 mL/day non-carbonated (p=0.004) and carbonated (p<0.001) sugar-sweetened beverages compared to the overweight or obese, who consumed ≥500 mL/day. Conclusions. The quantitative and qualitative assessment of fluid intake among adolescents is a mandatory step in the assessment of calorie and nutritional intake. Promoting low-calorie fluid intake in this age group, along with the principles of healthy eating, could contribute to achieving an optimal weight status.
The Prevalence of Diabetogenic Risk Factors in Newly Diagnosed Diabetic Patients
Authors: Mitrache Marilena , Ancuceanu Robert , Ionescu-Tîrgoviște Constantin 
Published: September 2015

Abstract
Background and Aims: The aim of this paper was the improvement of diabetes mellitus primary prevention through analysis of the prevalence of diabetogenic risk factors. Materials and Methods: The study group comprises 1590 newly diagnosed subjects with diabetes mellitus in a 24 month period in Ploiesti Municipal Hospital. We analyzed the prevalence in this population of some diabetogenic risk factors reported by different risk scores, including gender, age, body mass index (BMI), waist circumference (CA), physical activity at least 30 minutes a day, daily fruit and vegetable consumption, blood pressure history, family history of diabetes, etc. Results: Two-thirds of the patients declared a recent major stress. 74% had dyslipidemia at enrolment or hypolipidemic treatment. The presence of fetal macrosomia in the personal history was about 21%, from which 66% with a familial diabetes mellitus history. Conclusions: The risk factors` increased prevalence in diabetes mellitus detected in the analyzed sample population should determine an increased vigilance for an early screening of the people at risk, and to an early diagnosis of the disease.
Communication as a Key Issue in the Care of Diabetes Mellitus
Authors: Grigorescu Elena-Daniela , Lăcătuşu Cristina Mihaela , Botnariu Gina Eosefina , Popescu Raluca Maria , Popa Alina Delia , Onofriescu Alina , Mihai Bogdan-Mircea 
Published: September 2015

Abstract
The physician-patient communication has an essential role in establishing and supporting the relationship between these two partners. Moreover, modern medicine highlights the patient-centered approach. Publications assessing the impact of an efficient physicianpatient communication on medical care results in diseases such as diabetes and hypertension have revealed a positive correlation between patient’s satisfaction about the communication with the physician and values of blood pressure, glycated hemoglobin and pain intensity. Interventions needed in both doctors and patients for developing communication abilities were paid special attention in order to achieve an appropriate improvement in their communicative interaction during periodical appointments. In the field of diabetes mellitus, the medical challenge is to improve patients’ knowledge about medical care; this aim is achieved only by therapeutic education, using high-quality communication techniques.
Personal and Social Responsibility in Obesity
Authors: Arhire Lidia Iuliana 
Published: September 2015

Abstract
The health of a population is a social asset. The individual and the society contribute to it through biology and behaviour, environment and health systems. An ethical and responsible conduct from all parties is expected but difficult to legislate while seeking to respect personal and collective rights and freedoms. Out of all chronic diseases related to lifestyle, obesity is the best example to give when discussing if individuals are the only ones responsible for engaging in healthy behaviours or not, or whether environmental factors undermine their ability to act in their best interest and erode their freedom. It is still a controversial issue if the heath systems resources could be distributed according to the personal responsibility for health. The aim of this article is to review literature data about personal and social responsibility in relation to obesity.
Long-Lasting Gene Conversion Shapes the Convergent Evolution of the Critical Methanogenesis Genes
Authors: Wang Sishuo , Chen Youhua , Cao Qinhong , Lou Huiqiang 
Published: September 2015

AbstractMethanogenesis and its key small-molecule methyltransferase Mtr complex are poorly understood despite their pivotal role in Earth’s global carbon cycle. Mtr complex is encoded by a conserved mtrEDCBAFGH operon in most methanogens. Here we report that two discrete lineages, Methanococcales and Methanomicrobiales, have a noncanonical mtr operon carrying two copies of mtrA resulting from an ancient duplication. Compared to mtrA-1, mtrA-2 acquires a distinct transmembrane domain through domain shuffling and gene fusion. However, the nontransmembrane domains (MtrA domain) of mtrA-1 and mtrA-2 are homogenized by gene conversion events lasting throughout the long history of these extant methanogens (over 2410 million years). Furthermore, we identified a possible recruitment of ancient nonmethanogenic methyltransferase genes to establish the methanogenesis pathway. These results not only provide novel evolutionary insight into the methanogenesis pathway and methyltransferase superfamily but also suggest an unanticipated long-lasting effect of gene conversion on gene evolution in a convergent pattern.
Linkage Mapping Reveals Strong Chiasma Interference in Sockeye Salmon: Implications for Interpreting Genomic Data
Authors: Limborg Morten T , Waples Ryan K , Allendorf Fred W , Seeb James E 
Published: September 2015

AbstractMeiotic recombination is fundamental for generating new genetic variation and for securing proper disjunction. Further, recombination plays an essential role during the rediploidization process of polyploid-origin genomes because crossovers between pairs of homeologous chromosomes retain duplicated regions. A better understanding of how recombination affects genome evolution is crucial for interpreting genomic data; unfortunately, current knowledge mainly originates from a few model species. Salmonid fishes provide a valuable system for studying the effects of recombination in nonmodel species. Salmonid females generally produce thousands of embryos, providing large families for conducting inheritance studies. Further, salmonid genomes are currently rediploidizing after a whole genome duplication and can serve as models for studying the role of homeologous crossovers on genome evolution. Here, we present a detailed interrogation of recombination patterns in sockeye salmon (Oncorhynchus nerka). First, we use RAD sequencing of haploid and diploid gynogenetic families to construct a dense linkage map that includes paralogous loci and location of centromeres. We find a nonrandom distribution of paralogs that mainly cluster in extended regions distally located on 11 different chromosomes, consistent with ongoing homeologous recombination in these regions. We also estimate the strength of interference across each chromosome; results reveal strong interference and crossovers are mostly limited to one per arm. Interference was further shown to continue across centromeres, but metacentric chromosomes generally had at least one crossover on each arm. We discuss the relevance of these findings for both mapping and population genomic studies.
Whole-Genome Sequencing Suggests Schizophrenia Risk Mechanisms in Humans with 22q11.2 Deletion Syndrome
Authors: Merico Daniele , Zarrei Mehdi , Costain Gregory , Ogura Lucas , Alipanahi Babak , Gazzellone Matthew J , Butcher Nancy J , Thiruvahindrapuram Bhooma , Nalpathamkalam Thomas , Chow Eva W C , Andrade Danielle M , Frey Brendan J , Marshall Christian R , Scherer Stephen W , Bassett Anne S 
Published: September 2015

Abstract
Chromosome 22q11.2 microdeletions impart a high but incomplete risk for schizophrenia. Possible mechanisms include genome-wide effects of DGCR8 haploinsufficiency. In a proof-of-principle study to assess the power of this model, we used high-quality, whole-genome sequencing of nine individuals with 22q11.2 deletions and extreme phenotypes (schizophrenia, or no psychotic disorder at age &gt;50 years). The schizophrenia group had a greater burden of rare, damaging variants impacting protein-coding neurofunctional genes, including genes involved in neuron projection (nominal P = 0.02, joint burden of three variant types). Variants in the intact 22q11.2 region were not major contributors. Restricting to genes affected by a DGCR8 mechanism tended to amplify between-group differences. Damaging variants in highly conserved long intergenic noncoding RNA genes also were enriched in the schizophrenia group (nominal P = 0.04). The findings support the 22q11.2 deletion model as a threshold-lowering first hit for schizophrenia risk. If applied to a larger and thus better-powered cohort, this appears to be a promising approach to identify genome-wide rare variants in coding and noncoding sequence that perturb gene networks relevant to idiopathic schizophrenia. Similarly designed studies exploiting genetic models may prove useful to help delineate the genetic architecture of other complex phenotypes.
Proteomic Analysis of Dhh1 Complexes Reveals a Role for Hsp40 Chaperone Ydj1 in Yeast P-Body Assembly
Authors: Cary Gregory A , Vinh Dani B N , May Patrick , Kuestner Rolf , Dudley Aimée M 
Published: September 2015

AbstractP-bodies (PB) are ribonucleoprotein (RNP) complexes that aggregate into cytoplasmic foci when cells are exposed to stress. Although the conserved mRNA decay and translational repression machineries are known components of PB, how and why cells assemble RNP complexes into large foci remain unclear. Using mass spectrometry to analyze proteins immunoisolated with the core PB protein Dhh1, we show that a considerable number of proteins contain low-complexity sequences, similar to proteins highly represented in mammalian RNP granules. We also show that the Hsp40 chaperone Ydj1, which contains an low-complexity domain and controls prion protein aggregation, is required for the formation of Dhh1−GFP foci on glucose depletion. New classes of proteins that reproducibly coenrich with Dhh1−GFP during PB induction include proteins involved in nucleotide or amino acid metabolism, glycolysis, transfer RNA aminoacylation, and protein folding. Many of these proteins have been shown to form foci in response to other stresses. Finally, analysis of RNA associated with Dhh1−GFP shows enrichment of mRNA encoding the PB protein Pat1 and catalytic RNAs along with their associated mitochondrial RNA-binding proteins. Thus, global characterization of PB composition has uncovered proteins important for PB assembly and evidence suggesting an active role for RNA in PB function.
La minería ilegal arrasando las entrañas de la tierra
Authors: Pinilla Pedraza Claudia Patricia , Santos Morán Clara Yesenia 
Published: September 2015

<p>Este artículo presenta los resultados de un trabajo de grado de trabajo social sobre las percepciones y efectos de la minería ilegal en el río Cotuhé (norte del Trapecio Amazónico colombiano). Se presenta información de las percepciones de los indígenas tikuna de la comunidad de Caña Brava sobre el significado del territorio, la llegada de las balsas mineras al Cotuhé, los efectos ambientales y sociales de la minería, la impotencia de las organizaciones indígenas y el abandono por parte de las autoridades. La minería es percibida dentro de la comunidad de dos maneras contradictorias. Por una parte, es entendida como una bonanza que explota sus recursos naturales sin generar ningún beneficio económico y ocasionando graves daños ambientales y sociales. Por otra parte, algunos indígenas, sobre todo los más jóvenes, perciben la minería como una oportunidad laboral o económica para la comunidad. La actividad minera ha generado un desligamiento entre los miembros de la comunidad, mientras que las sustancias tóxicas empleadas por los mineros han expuesto peligrosamente a los habitantes de la comunidad, cuya fuente primaria de proteína es el consumo de peces. </p>
No solo crear la memoria de nosotros como pueblos indígenas, sino enseñar a ver esa memoria
Authors: Calambás Marisol 
Published: September 2015

<p>Marisol Calambás Soscué presenta su recorrido creativo como una búsqueda de nuevas técnicas y formas plásticas para expresar la memoria ancestral del pueblo Nasa al que pertenece. Es necesario escapar del estancamiento artificial impuesto por el mercado turístico que etiqueta las obras indígenas como ‘artesanías’ y exige la repetición de patrones supuestamente ancestrales. La creatividad de los artistas contemporáneos indígenas, muestra Marisol, fluye transformando constantemente los canales de comunicación de la memoria. Es necesario, por tanto, educar la mirada del público para que aprenda a ver dichas transformaciones. </p>