bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Peanut Seed Treatments for Organic Management in Georgia
Authors: Tubbs R. S. , Cantonwine E. G. , Brenneman T. B. 
Published: November 2013

ABSTRACT
One of the most critical and influential factors determining ultimate crop success is plant stand establishment. Because synthetic seed treatments are not allowed in organic production systems, alternatives are needed to assist in resistance of pathogens during germination and seedling growth. Several biological control materials were evaluated in laboratory assays and field trials to determine their potential for minimizing disease impact and maximizing yield in organic peanut production. These included Bacillus subtilis and several application methods of copper sulfate. Bacillus subtilis demonstrated excellent control of Aspergillus in the laboratory, but this was not confirmed in the field. Copper sulfate had no benefit against Aspergillus, but had some activity against Rhizopus in the laboratory. When applied dry to the seed, copper sulfate improved plant stands and reduced postemergence plant mortality (damping-off) compared to either B. subtilis or untreated seed. When applied in combination with cola as a sticking agent, copper sulfate did an excellent job of minimizing damping-off, but caused delayed emergence or a reduced plant stand compared to all other treatments. Because there was no evidence of direct toxicity against A. niger by the copper sulfate treatments in the lab assay, the field effect may be the result of enhanced host resistance to Aspergillus crown rot, or activity on another pathogen.
Evidence of rapid subglacial water piracy under Whillans Ice Stream, West Antarctica
Authors: Carter S.P. , Fricker H.A. , Siegfried M.R. 
Published: November 2013

AbstractThe subglacial water system of lower Whillans Ice Stream on the Siple Coast, West Antarctica, contains numerous connected subglacial lakes in three hydrological basins (northern, central and southern). We use Ice, Cloud and land Elevation Satellite (ICESat) data to derive estimates of lake volume change and regional thickness changes. By combining these results with a water budget model, we show that a uniform, localized thickness increase perturbed the hydropotential, resulting in a change in course of a major flow path within the system in 2005. Water originating from upper Whillans and Kamb Ice Streams that previously supplied the southern basin became diverted toward Subglacial Lake Whillans (SLW). This diversion led to a tenfold filling rate increase of SLW. Our observation suggests that water piracy may be common in the Siple Coast region, where the gentle basal relief makes the basal hydropotential particularly sensitive to small changes in ice thickness. Given the previously inferred connections between water piracy and ice-stream slowdown elsewhere in the region, the subtle and complex nature of this system presents new challenges for numerical models.
β2 Integrin–Mediated Crawling on Endothelial ICAM-1 and ICAM-2 Is a Prerequisite for Transcellular Neutrophil Diapedesis across the Inflamed Blood–Brain Barrier
Authors: Gorina Roser , Lyck Ruth , Vestweber Dietmar , Engelhardt Britta 
Published: November 2013

Abstract
In acute neuroinflammatory states such as meningitis, neutrophils cross the blood–brain barrier (BBB) and contribute to pathological alterations of cerebral function. The mechanisms that govern neutrophil migration across the BBB are ill defined. Using live-cell imaging, we show that LPS-stimulated BBB endothelium supports neutrophil arrest, crawling, and diapedesis under physiological flow in vitro. Investigating the interactions of neutrophils from wild-type, CD11a−/−, CD11b−/−, and CD18null mice with wild-type, junctional adhesion molecule-A−/−, ICAM-1null, ICAM-2−/− , or ICAM-1null/ICAM-2−/− primary mouse brain microvascular endothelial cells, we demonstrate that neutrophil arrest, polarization, and crawling required G-protein–coupled receptor–dependent activation of β2 integrins and binding to endothelial ICAM-1. LFA-1 was the prevailing ligand for endothelial ICAM-1 in mediating neutrophil shear resistant arrest, whereas Mac-1 was dominant over LFA-1 in mediating neutrophil polarization on the BBB in vitro. Neutrophil crawling was mediated by endothelial ICAM-1 and ICAM-2 and neutrophil LFA-1 and Mac-1. In the absence of crawling, few neutrophils maintained adhesive interactions with the BBB endothelium by remaining either stationary on endothelial junctions or displaying transient adhesive interactions characterized by a fast displacement on the endothelium along the direction of flow. Diapedesis of stationary neutrophils was unchanged by the lack of endothelial ICAM-1 and ICAM-2 and occurred exclusively via the paracellular pathway. Crawling neutrophils, although preferentially crossing the BBB through the endothelial junctions, could additionally breach the BBB via the transcellular route. Thus, β2 integrin–mediated neutrophil crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed BBB.
Cell-Intrinsic In Vivo Requirement for the E47–p21 Pathway in Long-Term Hematopoietic Stem Cells
Authors: Santos Patricia M. , Ding Ying , Borghesi Lisa 
Published: November 2013

Abstract
Major regulators of long-term hematopoietic stem cell (LT-HSC) self-renewal and proliferation have been identified, but knowledge of their in vivo interaction in a linear pathway is lacking. In this study, we show a direct genetic link between the transcription factor E47 and the major cell cycle regulator p21 in controlling LT-HSC integrity in vivo under repopulation stress. Numerous studies have shown that E47 activates p21 transcription in hematopoietic subsets in vitro, and we now reveal the in vivo relevance of the E47–p21 pathway by reducing the gene dose of each factor individually (E47het or p21het) versus in tandem (E47hetp21het). E47hetp21het LT-HSCs and downstream short-term hematopoietic stem cells exhibit hyperproliferation and preferential susceptibility to mitotoxin compared to wild-type or single haploinsufficient controls. In serial adoptive transfers that rigorously challenge self-renewal, E47hetp21het LT-HSCs dramatically and progressively decline, indicating the importance of cell-intrinsic E47–p21 in preserving LT-HSCs under stress. Transient numeric recovery of downstream short-term hematopoietic stem cells enabled the production of functionally competent myeloid but not lymphoid cells, as common lymphoid progenitors were decreased, and peripheral lymphocytes were virtually ablated. Thus, we demonstrate a developmental compartment–specific and lineage-specific requirement for the E47–p21 pathway in maintaining LT-HSCs, B cells, and T cells under hematopoietic repopulation stress in vivo.
Metastatic prostate cancer in transsexual diagnosed after three decades of estrogen therapy.
Authors: Turo Rafal , Jallad Samer , Cross William Richard , Prescott Stephen 
Published: November 2013

The incidence of prostate cancer in transsexual patients is very low with only few reported cases. Many years before presenting with prostate cancer, these patients receive hormone ablation as a part of their gender therapy. Their disease is already defined as castrate resistant, and the treatment and follow-up of such patients remains a challenge. We report a case of a male-to-female transgender woman who was diagnosed with metastatic prostate cancer, 31 years post-feminization.
Sexual function outcomes following fracture of the penis.
Authors: Nason Gregory J , McGuire Barry B , Liddy Stephen , Looney Aisling , Lennon Gerald M , Mulvin David W , Galvin David J , Quinlan David M 
Published: November 2013

Introduction: Fracture of the penis is a rare urological emergency which occurs as a result of abrupt trauma to an erect penis. There is paucity of data regarding long-term sexual function or erectile potency following fracture of the penis. The aim of this study is to objectively assess the overall sexual function following fracture of the penis.Methods: A retrospective analysis of 21 penile fractures was performed. A voluntary telephone questionnaire was performed to assess long term outcomes using three validated questionnaires-the Erection Hardness Grading Scale, the International Index of Erectile Function (IIEF-5) and the Brief Male Sexual Function inventory (BMSFI).Results: The mean age was 33.1 years (range: 19-63). The median follow up was 46 months (range: 3-144). All fractures were a result of sexual misadventure and all were surgically repaired. There were two concomitant urethral injuries. Seventeen patients were contactable. Fourteen patients demonstrated no evidence of erectile dysfunction (ED) (IIEF-5>22), 1 patient reported symptoms of mild ED (IIEF-5, 17-21) and one patient reported mild to moderate ED (IIEF-5, 12-16). No patients reported insufficient erection for penetration (EHGS: 1 or 2). Regarding the overall BMSFI, 13 (83%) patients were mostly satisfied or very satisfied with their sex life within the previous month.Conclusion: In a small surgical series of men with penile fracture managed within a short time frame from presentation, we demonstrate erectile potency is maintained. Long-term overall sexual satisfaction is promising.
Metabolic Activation and Inactivation of Irinotecan when Combined with the Human Monoclonal Antibody Bevacizumab
Authors: Martin Czejka  , Andreas Kiss  , Eva Ostermann  , Johannes Schueller  , Mansoor Ahmed  , Najia Mansoor  , Tasneem Ahmad  
Published: November 2013

Purpose: This pharmacokinetic study was designed to investigate whether the co-administration of the monoclonal antibody bevacizumab (BVC) shows potential to modulate the plasma disposition of irinotecan (CPT-11) and its metabolites.
Patients and Methods: Ten patients suffering from advanced colorectal cancer entered this pharmacokinetic study. Patients received CPT-11 as a 60 min i.v. - infusion (180 mg/m2, total dose 339 ± 32 mg) weekly for six weeks. BVC was administered biweekly as an intravenous 90 min infusion containing 5 mg BVC per kg body weight in 100 ml balanced sodium chloride solution. Pre-medication consisted of tropisetrone (3 mg i.v. push) and atropine (0.5 mg i.v.) one hour before CPT-11 infusion. Plasma samples were analysed during / after the first (MONO) and after the third CPT-11 infusion (BVC regimen).
Results: BVC did not alter plasma disposition and pharmacokinetics of the parent compound CPT-11, but in contrary BVC appeared to lower the plasma concentrations of the metabolites SN-38, SN-38gluc and APC.
Conclusion: Overall, our findings indicate that administration of BVC prior to chemotherapy showed no clinically significant impact on the pharmacokinetics and metabolic activation of CPT-11.
A DNA Vaccine Targeting the Fetal Liver Kinase-1 (Flk-1) can Activate the Special CD8+ T Cell and Inhibit the Metastasis of Orthotopic Lewis Lung Cancer Model
Authors: Xin Liu  , Yan Chen  , Zhi Ping Wu  , Cong Guo Jin  , Xiao Qun Chen  , Jia Li  , Yong Chun Zhou  , Xi Cai Wang  
Published: November 2013

Lung cancer is the leading cause of cancer related deaths and need new more effective therapies. In this study, we investigated the anti-tumor effect of recombinant orally DNA vaccine delivered by attenuated S.typhimurium strain SL3261 (aroA mutant) targeting vascular endothelial growth factor receptor (VEGFR-2), also known as fetal liver kinase-1 (Flk-1) in mouse. The cDNA of extracellular domains (ECD) of VEGFR-2 (Flk-1ECD) was amplified by RT-PCR and cloned into the pcDNA3.1 (+) vector, then transformed to the attenuated S.typhimurium strain to construct the oral DNA vaccine. Then pcDNA3.1-Flk-1ECD was successfully transfected into COS-7 cells and the recombinant protein was detected by Western blot. The effect of the oral DNA vaccine was analyzed by flow cytometry (FCM) analysis and cytotoxicity assay. For mimic the local and regional growth pattern seen in lung cancer patients, the effect of the oral DNA vaccine on tumor growth and metastasis was analyzed by orthotopic cancer cells challenge in vivo. The results demonstrated that the oral DNA vaccine can overcome peripheral immune tolerance, and generated Flk-1- specific CD8+ cytotoxic T cell response. Moreover, this oral DNA vaccine could effectively reduce tumor growth, metastasis and increase the survival. It indicated that the oral VEGFR2 DNA vaccine encoding Flk-1ECD delivered by salmonella might act a potential strategy for immunotherapy of lung cancers.
Making sense of wildlife habitat use on active oil sands mines: Quasi‐experiments, occupancy models, trends assessments and upland habitat reclamation
Authors: Hawkes Virgil C. , Gibeau Pascale , Challenger Wendell 
Published: July 2024

Abstract

Intensive resource extraction activity in the oil sands of Canada alters the quantity, structure and distribution of native ecosystems, which in turn creates substantive challenges for conservation, land management and habitat reclamation. Progressive reclamation occurs on active mine sites in the Athabasca oil sands region (AOSR) of Canada, along with concurrent assessments of reclamation effectiveness. Yet, little is known about the ability of reclaimed habitats to mitigate for the short‐ and long‐term impacts associated with open‐pit mines and provide functional habitat for wildlife.

We used a robust quasi‐experiment that combined camera trap data from an observational study with an innovative occupancy model to assess the effectiveness of upland reclamation to provide habitat for wildlife in the AOSR. The dynamic occupancy models were applied to 15 years of camera trap data to assess wildlife usage patterns of nine species of wildlife over seven types of habitats. The habitats sampled ranged from mining‐disturbed habitats reclaimed to upland forest ecosites common in the region, habitats disturbed by natural (fire) and human disturbances (clear‐cut logging), with comparisons to mostly intact mature forests.

Our results indicate that the nine species of wildlife assessed used habitats in a manner consistent with expectations: some preferred disturbance‐dominated habitats while others used mature forest to a higher degree. We demonstrate that the application of dynamic occupancy models to camera trap data reliably discerned these trends, providing the means to predict wildlife usage patterns in an ever‐changing landscape, including one containing bitumen extraction as a contributor to landscape‐level modifications.

Practical implication: Our work illustrates how continued monitoring of wildlife using camera traps contributes to assessments of reclamation effectiveness with respect to wildlife occurrence, distribution and usage patterns in anthropogenically and naturally disturbed landscapes. Evaluating the effectiveness of reclamation is especially important given the expected increase in habitat reclamation on active oil sands mines over the next several decades, coupled with the need to ensure that disturbed habitats regain ecological function able to sustain wildlife in the future.
Emerging Solid–State Thermal Switching Materials
Authors: Jia Junjun , Li Shuchen , Chen Xi , Shigesato Yuzo 
Published: July 2024

AbstractGrowing technical demand for thermal management stems from the pursuit of high–efficient energy utilization and the reuse of wasted thermal energy, which necessitates the manipulation of heat flow with electronic analogs to improve device performance. Here, recent experimental progress is reviewed for thermal switching materials, aiming to achieve all–solid–state thermal switches, which are an enabling technology for solid–state thermal circuits. Moreover, the current understanding for discovering thermal switching materials is reshaped from the aspect of heat conduction mechanisms under external controls. Furthermore, current challenges and future perspectives are provided to highlight new and emerging directions for materials discovery in this continuously evolving field.
Strategic Fabrication of Au4Cu2 NC/ZIF‐8 Composite Via In Situ Integration Technique for Enhanced Energy Storage Applications
Authors: Ahmad Muhammad , Nawaz Tehseen , Eddahani Yassine , Hussain Iftikhar , Chen Xi , Low Kam Hung , He Jian , Zhang Kaili 
Published: July 2024

AbstractMetal–organic frameworks (MOFs), known for their extensive porosity and versatile crystallinity, play a crucial role in the development of advanced energy storage materials. However, their application is limited by stability and conductivity issues. This study addresses these challenges by integrating ultrasmall metal nanoclusters, specifically Au4Cu2 NC, synthesized using a mixed ligand strategy combining 2, 4‐Dimethyl benzene thiol (2,4‐DMBTH) and 1,2‐bis(diphenylphosphino)ethane (dppe). The bimetallic Au4Cu2 NC, characterized by Single Crystal X‐Ray Diffraction (SCXRD), is applied to zeolitic imidazolate framework‐8 (ZIF‐8) using both in situ and ex situ methods to explore their electrochemical and physicochemical properties in energy storage. The in situ Au4Cu2 NC/ZIF‐8 composite demonstrated a specific capacitance that is almost two times higher than its ex situ counterpart, attributed to homogeneous dispersion and hence enhanced conductivity. This in situ integration of atomically precise bimetallic nanoclusters on MOFs significantly boosts supercapacitor performance, offering a more effective and reliable solution for energy storage. Further, in practical applications, this device demonstrated an energy density of 87.2 Wh kg−1 at a power density of 1474 W kg−1, highlighting its robustness and potential for high‐performance energy storage applications. This approach effectively combats the issue of nanocluster aggregation on substrates, marking a significant progression in supercapacitor technology.
Current‐Adaptive Li‐Ion Storage Mechanism in High‐Rate Conversion‐Alloying Metal Chalcogenides
Authors: Li Wangyang , Deng Liying , Cao Jiaqi , Ke Bingyuan , Wang Xinghui , Ni Shibing , Cheng Shuying , Lu Bingan 
Published: July 2024

AbstractBeyond the high theoretical capacity, conversion‐alloying metal chalcogenides (CAMCs) exhibit exceptional high‐rate performance as Li‐ion battery electrodes. However, the inherent origin of the high‐rate performance remains elusive, especially given the lower intrinsic conductivity of CAMCs. Here, the correlation between phase evolution and charge transport dynamics in fully activated CAMCs is systematically investigated, elucidating a current‐adaptive Li‐ion storage mechanism to explain the anomalous high‐rate performance. Briefly, the deconversion reaction manipulated by ion diffusion acts as a “regulator” to adaptively modulate the transition from metal (high electronic conductivity) and lithium chalcogenides (high ionic conductivity) to CAMCs, thus removing the charge transport bottleneck without affecting the formation of the metal feedstock required for the alloying reaction. On this basis, the high capacity can be maintained at high rates through a “fading‐free” alloying reaction. This study offers a novel perspective for the design of high‐rate electrode materials.
Manipulation of Linked Phosphorus Chemistry to Enhance the Battery Performance of Phenazine‐Based Porous Polymers
Authors: Zeng Jing , Chen Ling , Zhang Sen , Xing Fangfang , Li Hongyan , Wang Xiujuan , He Xiaoming 
Published: July 2024

AbstractOrganic p‐type cathodes have captured increasing attention for lithium‐organic batteries due to their high voltage output. However, because of the low electronic and ionic conductivity, the p‐type organic electrode materials often show sluggish reaction kinetics and hence low capacities and poor cyclability. In this work, three conjugated polymers (Pz‐TPPO, Pz‐TPPS, and Pz‐TPP) based on p‐type phenazine linked with triphenyl phosphine are reported with different chemical modifications of phosphorus atom. Through oxidation and sulfuration of phosphorus atoms, the electron‐accepting property strength is increased, resulting in higher electronic conductivity of Pz‐TPPO and Pz‐TPPS. It is also found that the P═O unit in Pz‐TPPO exhibits stronger Li+ coordination, which can bring anions into close proximity to the redox centers, further improving the anion shuttling during charge and discharge. All these lead to the best lithium‐organic battery performance of Pz‐TPPO, such as high voltage output (3.1–3.9 V), high reversible specific capacity (149 mAh g−1 at 0.2 A g−1) and excellent cycle stability. The potential application has also been demonstrated in Pz‐TPPO//graphite full battery. This work provides a novel strategy for designing donor–acceptor (D–A) conjugated polymer by simple phosphorus modification toward high‐performance organic batteries.
Donor Sex and Passage Conditions Influence MSC Osteogenic Response in Mineralized Collagen Scaffolds
Authors: Kolliopoulos Vasiliki , Tiffany Aleczandria , Polanek Maxwell , Harley Brendan A. C. 
Published: July 2024

AbstractContemporary tissue engineering efforts often seek to use mesenchymal stem cells (MSCs) due to their multi‐potent potential and ability to generate a pro‐regenerative secretome. While many have reported the influence of matrix environment on MSC osteogenic response, few have investigated the effects of donor and sex. Here, a well‐defined mineralized collagen scaffold is used to study the influence of passage number and donor‐reported sex on MSC proliferation and osteogenic potential. A library of bone marrow and adipose tissue‐derived stem cells from eight donors to examine donor viability in osteogenic capacity in mineralized collagen scaffolds is obtained. MSCs displayed reduced proliferative capacity as a function of passage duration. Further, MSCs showed significant sex‐associated variability in osteogenic capacity. Notably, MSCs from male donors displayed significantly higher cell proliferation while MSCs from female donors displayed significantly higher osteogenic response via increased alkaline phosphate activity, osteoprotegerin release, and mineral formation in vitro. The study highlights the essentiality of including donor‐reported sex as an experimental variable and reporting culture expansion in future studies of biomaterial regenerative potential.
Tissue/Organ Adaptable Bioelectronic Silk‐Based Implants
Authors: Zhu Ziyi , Yan Zhiwen , Ni Siyuan , Yang Huiran , Xie Yating , Wang Xueying , Zou Dujuan , Tao Chen , Jiang Wanqi , Jiang Jianbo , Su Zexi , Xia Yuxin , Zhou Zhitao , Sun Liuyang , Fan Cunyi , Tao Tiger H. , Wei Xiaoling , Qian Yun , Liu Keyin 
Published: July 2024

AbstractImplantable bioelectronic devices, designed for both monitoring and modulating living organisms, require functional and biological adaptability. Pure silk is innovatively employed, which is known for its excellent biocompatibility, to engineer water‐triggered, geometrically reconfigurable membranes, on which functions can be integrated by Micro Electro Mechanical System (MEMS) techniques and specially functionalized silk. These devices can undergo programmed shape deformations within 10 min once triggered by water, and thus establishing stable bioelectronic interfaces with natively fitted geometries. As a testament to the applicability of this approach, a twining peripheral nerve electrode is designed, fabricated, and rigorously tested, demonstrating its efficacy in nerve modulation while ensuring biocompatibility for successful implantation.