bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome
Authors: Li John T. , Melton Andrew C. , Su George , Hamm David E. , LaFemina Michael , Howard James , Fang Xiaohui , Bhat Sudarshan , Huynh Kieu-My , O’Kane Cecilia M. , Ingram Rebecca J. , Muir Roshell R. , McAuley Daniel F. , Matthay Michael A. , Sheppard Dean 
Published: May 2015

Abstract
Acute respiratory distress syndrome (ARDS) is a devastating disorder characterized by increased alveolar permeability with no effective treatment beyond supportive care. Current mechanisms underlying ARDS focus on alveolar endothelial and epithelial injury caused by products of innate immune cells and platelets. However, the role of adaptive immune cells in ARDS remains largely unknown. In this study, we report that expansion of Ag-specific αβTh17 cells contributes to ARDS by local secretion of IL-17A, which in turn directly increases alveolar epithelial permeability. Mice with a highly restrictive defect in Ag-specific αβTh17 cells were protected from experimental ARDS induced by a single dose of endotracheal LPS. Loss of IL-17 receptor C or Ab blockade of IL-17A was similarly protective, further suggesting that IL-17A released by these cells was responsible for this effect. LPS induced a rapid and specific clonal expansion of αβTh17 cells in the lung, as determined by deep sequencing of the hypervariable CD3RβVJ region of the TCR. Our findings could be relevant to ARDS in humans, because we found significant elevation of IL-17A in bronchoalveolar lavage fluid from patients with ARDS, and rIL-17A directly increased permeability across cultured human alveolar epithelial monolayers. These results reveal a previously unexpected role for adaptive immune responses that increase alveolar permeability in ARDS and suggest that αβTh17 cells and IL-17A could be novel therapeutic targets for this currently untreatable disease.
Evaluation of meat quality after application of different feed additives in diet of broiler chickens
Authors: Haščí­k Peter , Trembecká Lenka , Bobko Marek , Čuboň Juraj , Bučko Ondřej , Tkáčová Jana 
Published: May 2015

The present study was conducted to investigate the effect of natural feed additives, namely bee pollen extract, propolis extract and probiotic preparation, on technological properties of meat in order to evaluate the meat quality of Ross 308 broiler chickens. The feeding of chickens (180 pcs) lasted for a period of 42 days. The experiment was carried out without segregation between the genders. The chickens were randomly divided into 4 groups. The control group was fed a basal diet, whereas the other three groups were fed diets supplemented with natural additives, i.e. bee pollen extract at level of 400 mg.kg-1 of feed mixture, propolis extract at level of 400 mg.kg-1 of feed mixture, and probiotic preparation based on Lactobacillus fermentum (1.109 CFU per 1 g of bearing medium) in an amount of 3.3 g added to water (for 30 pcs chickens until 21 days of age, for 20 pcs chickens from 22nd to 42nd day of age) given to group E1, group E2 and group E3, respectively. The feed mixtures were produced without any antibiotic preparations and coccidiostatics. During the whole period of experiment, the broiler chickens had ad libitum access to feed and water. The following technological properties were examined: cooling loss (after 24 h of storage at 4 °C), freezing loss (after 3 months of storage at -18 °C), roasting loss (performed on roasted meat that was stored at -18 °C for 3 months before thawing), colour parameters based on CIELab system (the L*, a*, b* values of raw breast and thigh muscle), and tenderness (as shear force of roasted breast and thigh muscle). We have made a finding, that the examined additives had only little impact on meat quality in most of the investigated parameters, except the significant increase (p ≤0.05) in redness (a*) values and the slight decrease in roasting loss and shear force determination after propolis extract supplementation. Therefore, it may be inferred that propolis extract has been shown as the most appropriate feed additive among the applied supplements.
Oxidative stability of chicken meat during storage influenced by the feeding of alfalfa meal
Authors: Tkáčová Jana , Angelovičová Mária , Haščí­k Peter , Bobko Marek 
Published: May 2015

The aim of our experiment was to determine the effect of the alfalfa meal component in feed mixtures of Ross broiler chickens on oxidative stability of meat. Proportion of alfalfa meal in feed mixtures was 4 and 6%. The results were compared to the control group without alfalfa meal in feed mixtures. At the end of the experiment (day 38), 6 pcs of broiler chickens from each group with an average live body weight over 1 800 g were randomly selected. The samples for chemical analysis consisted of identical proportion of breast and thigh muscle, and about 1 cm2 of skin with subcutaneous fat. Fat from the meat was obtained after the samples drying. A fat was determined by extraction by means of laboratory instrument Det N Gras Selecta P. The oxidative stability of meat on the basis of acid number of fat was determided by chemical analysis. Chicken meat was stored at -18 °C for 12 months and 18 months. The acid number of fat of stored meat for 12 months was 7.38 mg KOH per g in the control group, 7.42 mg KOH per g in the group with a proportion of 4% alfalfa meal, and 11.18 mg KOH per g in the group with proportion 6% alfalfa meal. An acid number of fat of stored meat for 18 months was 5.90 mg KOH per g in the control group, 4.65 mg KOH per g in the group with a proportion of 4% alfalfa meal, and 7.07 mg KOH per g in the group with a proportion of 6% alfalfa meal. Chicken meat is notably sensitive to lipid oxidation because of its high content of polyunsaturated fatty acids. Legislation in Title 5 of Part 3 of the Codex Alimentarius of the Slovak Republic and the Government Regulation No. 286/2003 Coll. in the Annex 4 in Part B provide the requirements for animal fats and meat products. Regulation of the European Parliament and Council (EC) No. 853/2004 lays down specific hygiene rules for food of animal origin. In particular, determination of free fatty acids content of rendered animal fat (tallow, lard, other animal fat). Legislative regulation does not contain requirements for the quality of chicken meat, the acid number of fat of fresh or frozen chicken meat, respectively. A chicken meat is preferred over other kinds of meat. It is characterized by certain dietary and nutritional properties that consumer prefers. A price of this kind of meat remains attractive. In terms of human health, oxidative stability of chicken meat is important, especially of stored meat. In general terms, the various food additives are currently used to maintain the food stability. Great attention is currently paid to additives of natural origin. Similar focus is presented in our study. We can state, on the basis of the oxidative stability results of chicken meat, that natural feed component has its justification. This issue requires further research.
Quality of meat of rabbits after application of epicatechin and patulin
Authors: Kalafová Anna , Bulla Jozef , Bučko Ondrej , Emrichová Jana , Zbyňovská Katarí­na , Petruška Peter , Schneidgenová Monika , Chrastinová Ľubica , Ondruška Ľubomí­r , Jurčí­k Rastislav , Mellen Martin , Capcarová Marcela 
Published: May 2015

The aim of the present study was to determinate the effect of epicatechin and patulin on selected parameters of meat quality of rabbits. Adult female rabbits (n=25), maternal albinotic line (crossbreed Newzealand white, Buskat rabbit, French silver) and paternal acromalictic line (crossbreed Nitra's rabbit, Californian rabbit, Big light silver) were used in experiment. Animals were divided into five groups: control group (C) and experimental groups E1, E2, E3, and E4. Animals from experimental groups E1, E2, E3, E4 received patulin through intramuscular injection (10 µg.kg-1) twice a week and animals from groups E2, E3, E4 received epicatechin three times a week through intramuscular injection. After 30 days animals were slaughtered. For analysing of meat quality the samples of Musculus longissimus dorsi (50 g) were used. Application of epicatechin and patulin to rabbits had slight or no effect on the pH levels in stomach, small intestine, large intestine and urinary bladder contents, however differences among the groups were insignificant (p ˃0.05). Application of epicatechin and patulin to rabbits had slight or no effect on total water, protein, fat and differences among the groups were insignificant (p >0.05). The values of amino acids concentrations were not influenced after application of epicatechin and patulin. The fatty acid profiles in animals after application of different doses of epicatechin and 10 µg.kg-1 patulin were similar (p >0.05). Concentration of cholesterol increased in experimental groups in comparison with the control group, but differences were insignificant (p >0.05). pH levels of meat of rabbits in experimental group E3 was lower when compared with the control group, but differences was not significant (p >0.05). Electric conductivity parameter was increased in each experimental group (in E3 the highest) against the control but without significant differences (p >0.05). Colour L parameter was slightly decreased in experimental groups with comparison to the control group (in E3 the lowest). Generally we can conclude that intramuscular application of epicatechin or patulin did not affect parameters of meat quality as well as pH values of internal organs content. Further investigations are needed to prove the final answer concerning the health promoting effects of epicatechin and patulin.
Selected parameters of quality and safety of herbal tea
Authors: Bobková Alica , Fikselová Martina , Bobko Marek , Lopašovský Ľubomí­r , Tóth Tomáš , Zeleňáková Lucia 
Published: May 2015

The aim of this work was to assess the heavy metal presence and possible microbiological contamination in herbal teas. Evaluation of selected tea products was performed from Nitra locality during years 2009 - 2013. Microscopic filamentous fungi detection, bacteria such as Escherichia coli and Salmonella spp. were compared to requirements given in the Codex Alimentarius of Slovakia. The highest permissible limit for microscopic filamentous fungi was not exceeded (in 32 observed herbal tea samples). For incidence of Escherichia coli, 93 samples were investigated and for Salmonella spp., 91 herbal tea samples. No sample showed the presence of Salmonella spp., and at E. coli maximum permitted presence was detected below limit. Among chemical parameters, cadmium, lead and mercury content were monitored. The highest amount of lead and mercury was found in year 2012. In 2009, the highest cadmium content was found. The average content of lead in all 100 inspected herbal tea samples was 0.784 mg.kg-1 so all the samples met requirements defined in the legislation. The mean content of mercury (98 investigated herbal tea samples) was 0.0161 mg.kg-1 so all samples met the requirements as well. Average cadmium content was 0.1702 mg.kg-1 while the highest permitted limit for cadmium is 1.0 mg.kg-1. All herbal tea samples were in accordance with the legislation except one (white willow bark tea) with a very high content of cadmium (4.36 mg.kg-1).
Health safety aspects of foodstuffs intented for phenylketonurics
Authors: Matejová Stanislava , Fikselová Martina , Buňka František 
Published: May 2015

Phenylketonuria is a rare metabolic disorder that occurs due to the lack of liver enzyme phenylalanine hydroxylase. Untreated it leads to mental retardation, delayed development of speech, microcephaly, epilepsy, behavioral problems etc. Its treatment consists of strict diet, very low in phenylalanine content. The aim of this study was to determine the health safety and suitability of 17 selected foods labeled as „low in protein" respectively „foods low in phenylalanine". Analyses were focused on their phenylalanine content and aminoacid representation. The other aim was to compare phenylalanine measured with the content declared by the producers on their label. By product origin, the largest amount of foodstuffs originated from Poland (59%), followed by Germany (23%) and equal representation (6%) had Slovakia, Hungary and Sweden. Automatic analyzer AAA 400, using ion exchange chromatography, analyzed the samples. The lowest determined phenylalanine content was 1 mg.100g-1 (in rubber candy) and the highest 299 mg.100g-1 (in paté). Powdered egg alternative was monitored as the second highest source of phenylalanine. Phenylalanine content in the monitored foods ranged from 1 to 299.6 mg. 100 g-1 respectively, and increased in the following order: Gum candies (1 mg.100g-1) < Cherry jelly (1.4 mg. 100g-1) < Flour (5.8 mg.100g-1) < Flour (5.9 mg.100g-1) < Spaghetti (8.7 mg.100g-1) < Pasta (9.9 mg.100g-1) < Waffle (14.2 mg.100g-1) < Salty sticks (23.8 mg.100g-1) < Chocolate (24.1 mg.100g-1) < Bread (26 mg.100g-1) < Breadcrumbs (27.1 mg.100g-1) < Sticks with salt (30.3 mg.100g-1) < Ice Cornets (30.8 mg.100g-1) < Walnut cookie (37.2 mg.100g-1) < Instant soup with noodles (46.1 mg.100g-1) < Powdered egg alternative (58 mg.100g-1) < Pate (299.6 mg.100g-1). Only seven products of 17 observed showed low phenylalanine content, less than 20 mg.100g-1.
Colonization of grapes berries and cider by potential producers of patulin
Authors: Tančinová Dana , Felšöciová Soňa , Rybárik Ľubomí­r , Mašková Zuzana , Cí­sarová Miroslava 
Published: May 2015

The aim of this study was to detect potential producers of mycotoxin patulin from grapes (berries, surface sterilized berries - endogenous mycobiota and grape juice) of Slovak origin. We analyzed 47 samples of grapes, harvested in 2011, 2012 and 2013 from various wine-growing regions. For the isolation of species we used the method of direct plating berries and surface-sterilized berries (using 1% freshly pre-pared chlorine) berries on DRBC (Dichloran Rose Bengal Chloramphenicol agar). For the determination of fungal contamination of grape juice we used plate-dilution method and DRBC and DG18 (Dichloran 18% Glycerol agar) as media. The cultivation in all modes of inoculation was carried at 25 ±1 °C, for 5 to 7 days. After incubation Aspergillus and Pencillium isolates were inoculated on the identification media. The potential producers of patulin were isolated from 23 samples berries, 19 samples of surface-sterilized berries and 6 samples of grape juice. Overall, the representatives of producers of patulin were detected in 32 (68.1%) samples (75 isolates). In this work we focused on the detection of potential producers of patulin, Penicillium expansum (the most important producer of patulin in fruits), Penicillium griseofulvum and Aspergillus clavatus were isolated. Chosen isolates of potential patulin producers were tested for the ability to produce relevant mycotoxins in in vitro conditions using thin layer chromatography method. The ability to produce patulin in in vitro condition was detected in 82% of isolates of Penicillium expansum, 65% of Penicillium griseofuvum and 100% of Aspergillus clavatus. Some isolates of Penicillium expansum were able to produce citrinin and roquefortine C, Penicillium griseofulvum cyclopiazonic acid, griseofulvin and roquefortin C, also.
Evaluation of microbiological quality of selected cheeses during storage
Authors: Kunová Simona , Kačániová Miroslava , Čuboň Juraj , Haščí­k Peter , Lopašovský Ľubomí­r 
Published: May 2015

The aim of this article was to evaluate and compare the microbiological quality of selected types of cheeses immediately after opening and after 5 days storage in the refrigerator. Total viable counts (TVC), coliform bacteria (CB) and microscopic filamentous fungi (MFF) were determined by microbiological analysis. We analyzed 8 samples of cheese of Slovak origin. Plate dilution method was used for microbiological analysis. The Codex Alimentarius of Slovak republic (2006) just indicates number of coliforms bacteria (102) and microscopic fungi (5 × 102). The TVC values after opening of cheeses ranged from 1.68 × 103 CFU.g-1 (3.22 log CFU.g-1) in the sample no. 1 to 1.71 × 105 KTJ.g-1 (5.23 log CFU.g-1) in the sample no. 4 after storage in the refrigerator. All samples were negative for the presence of coliform bacteria after opening. The values of CB were 1.18 × 102 CFU.g-1 (2.07 log CFU.g-1) in sample no. 7 and 1.90 × 102 CFU.g-1 (2.27 log CFU.g-1) in the sample no. 8 after storage in refrigerator. These values are not in accordance with Codex Alimentarius of Slovak Republic (2006). Other samples were negative for presence of CB after storage at 4 °C. The values of MFF in samplesranged from 1.81 × 101 CFU.g-1 (1.25 log CFU.g-1) in the sample no. 1 after opening to 1.68 × 102 CFU.g-1 (2.22 log CFU.g-1) in sample no. 7 after storage of samples. All analysed samples were in accordance with Codex Alimentarius of Slovak republic (2006).
Rep-PCR typing of Staphylococcus spp. strains in meat paste production line and identification of their origin
Authors: Manga Ivan , Klimešová Marcela , Horáček Jiří­ , Koláčková Ivana , Bjelková Marie , Poní­žil Antoní­n , Nejeschlebová Ludmila 
Published: May 2015

A meat paste production line and its microbial parameters have been evaluated in single Czech company. The raw meat paste samples before heat treatment were tested positively for the presence of three staphylococci species: Staphylococcus aureus, Staphylococcus haemolyticus and Staphylococcus epidermidis. Subsequent microbial analysis of meat paste components and ingredients (fresh meat, water, spices, equipment) identified only the spices used as positive for S. aureus (coriander, cinnamon, badian, mustard – (10 - 40 cfu/g)) and S. haemolyticus strains (juniper, ginger). The collection of sixteen collected strains (S. aureus (n = 4), S. haemolyticus (n = 4), S. epidermidis (n = 8)) has been typed with the rep-PCR method utilising (GTG)5 primer. Analysis of the fingerprints using the unweighted pair-group method using arithmetic averages (UPGMA) clustering method revealed presence of eleven strain clusters with similarity lower than 90%: two fingerprint clusters of S. aureus, three individual clusters characteristic for S. haemolyticus and six different S. epidermidis specific clusters. The S. aureus strains from different types of spice were identical, resp. very similar. Molecular tracking composed from the rep-PCR analysis of acquired isolates and comparison among all collected fingerprints confirmed the spices to be the source of both S. aureus and S. haemolyticus strains identified in raw meat paste. The additional rep-PCR analysis of the S. epidermidis collection confirmed usability and performance of this method. The antibiotic susceptibility to fourteen individual antibiotics has been examined among the collected staphylococci strains. The predominant erythromycin resistance (68.8%) was followed with the resistance to amoxicillin/clavulanic acid (56.2%). Other resistances observed were less frequent (clindamycin – 12.5%, oxacillin – 6.3%, tetracycline – 6.3%, sulphamethoxazole-trimethoprim – 6.3%, chloramphenicol – 6.3%, novobiocin – 6.3%). As shown by our experimental results, rep-PCR with the (GTG)5 primer is an applicable tool for typing of bacterial strains and may be used for identifying the source of contamination.
Bioinformatic approach in the identification of arabidopsis gene homologous in amaranthus
Authors: Žiarovská Jana , Záhorský Michal , Gálová Zdenka , Hricová Andrea 
Published: May 2015

Bioinfomatics offers an efficient tool for molecular genetics applications and sequence homology search algorithms became an inevitable part for many different research strategies. Appropriate managing of known data that are stored in public available databases can be used in many ways in the research. Here, we report the identification of RmlC-like cupins superfamily protein DNA sequence than is known in Arabidopsis genome for the Amaranthus - plant specie where this sequence was still not sequenced. A BLAST based approach was used to identify the homologous sequences in the nucleotide database and to find suitable parts of the Arabidopsis sequence were primers can be designed. In total, 64 hits were found in nucleotide database for Arabidopsis RmlC-like cupins sequence. A query cover ranged from 10% up to the 100% among RmlC-like cupins nucleotides and its homologues that are actually stored in public nucleotide databases. The most conserved region was identified for matches that posses nucleotides in the range of 1506 up to the 1925 bp of RmlC-like cupins DNA sequence stored in the database. The in silico approach was subsequently used in PCR analysis where the specifity of designed primers was approved. A unique, 250 bp long fragment was obtained for Amaranthus cruentus and a hybride Amaranthus hypochondriacus x hybridus in our analysis. Bioinformatic based analysis of unknown parts of the plant genomes as showed in this study is a very good additional tool in PCR based analysis of plant variability. This approach is suitable in the case for plants, where concrete genomic data are still missing for the appropriate genes, as was demonstrated for Amaranthus.
New Dimensions in Nigerian Women's Pentecostal experience: The Case of DODIM, Nigeria
Authors: Olademo Oyeronke 
Published: May 2015

ABSTRACT
The place and role of women in Christianity have been marked by dynamic experiences in Nigeria. It is suffice to note that the gender paradigm at the advent of Christianity in Nigeria is no longer tenable. This is due mainly to the influence of culture as a potent negotiating tool for women in the church. Presently, women have gained remarkable visibility in the church in Nigeria through roles including presiding clergy, church founders, choir leaders and church council members to mention a few.
However, a new dimension to the experience of women in their interaction with the divine is the Daughters of Deborah International Ministry, Ilorin, Kwara State, Nigeria. The ministry provides avenues for women from the three broad divisions of churches in Nigeria to access the divine. These classifications of churches are: Orthodox/Mission, African Independent, and Pentecostal/Charismatic churches. DODIM as the ministry is known creates forum of meetings for women from these different churches in a non-structural setting. Again, these forums are managed by women for women focussing mainly on women issues. This provides a rare opportunity for women solidarity, networking and experience sharing in Christianity.
Methodology for this paper will be phenomenological with interviews and participant observations as tools of research. This paper attempts to contribute significantly to literature on women in religion in Nigeria.