bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct medical resource utilization associated with osteoporosis-related nonvertebral fractures in postmenopausal women
Authors: Jean Sonia , Bessette Louis , Belzile Étienne L , Davison K Shawn , Candas Bernard , Morin Suzanne , Dodin Sylvie , Brown Jacques P 
Published: September 2012

Abstract
The purposes of this study were to assess direct medical resource utilization related to the treatment of nonvertebral osteoporotic fractures within 1 year postfracture and to evaluate whether age impacts resource utilization. A previously-validated algorithm for physician claims databases identified 15,327 women aged 50 years or older with incident fracture at nonvertebral osteoporotic sites between January 1, 2004 and December 31, 2005. Administrative databases of the health services available to all residents in Quebec served to study fracture-related health resource utilization in the year after fracture. Data were linked by a unique personal identifier, creating a longitudinal cohort of all fracture cases for health resource utilization. The proportions of fractures treated by open reduction, closed reduction, immobilization or follow-up by an orthopedic surgeon (OS) were evaluated. The mean number of claims for consultation with an OS or other clinicians in inpatient and outpatient visits, the hospitalization rate and length of stay (LOS) were assessed. Hip/femur fractures represented the highest rate of resource utilization because the majority of them required surgery (91.1%) and hospitalization (94.5%) with a mean (median) LOS of 39.2 (31) days. However, other nonvertebral fracture types needed significant clinical care related to surgery (27.9%), follow-up consultation with an OS (77.6%), and hospitalization (27.3% of total LOS). Even pelvic fractures, which often do not require surgical treatment, commanded high resource utilization due to the high hospitalization rate (67.4%) with mean (median) LOS of 34.2 (26) days. Moreover, age was an important determinant of health resource utilization, being associated with an increased number of visits to other physicians, hospitalization, and length of hospitalization (LOS), admissions to long term care (LTC), and death. Osteoporosis-related fractures accounted for substantial healthcare resource utilization. With an aging population and increased prevalence of fractures, strategies for osteoporosis management need to be introduced to reduce the healthcare burden. © 2013 American Society for Bone and Mineral Research
Meta-analysis of vertebral augmentation compared with conservative treatment for osteoporotic spinal fractures
Authors: Anderson Paul A , Froyshteter Alexander B , Tontz William L 
Published: September 2012

Abstract
Cement augmentation is a controversial treatment for painful vertebral compression fractures (VCF). Our research questions for the meta-analysis were: Is there a clinical and statistical difference in pain relief, functional improvement, and quality of life between conservative care and cement augmentation for VCF and, if so, are they maintained at longer time points? We conducted a search of MEDLINE from January 1980 to July 2011 using PubMed, Cochrane Database of Systematic Reviews and Controlled Trials, CINAHL, and EMBASE. Searches were performed from Medical Subject Headings. Terms “vertebroplasty” and “compression fracture” were used. The outcome variables of pain, functional measures, health-related quality of life (HRQOL), and new fracture risk were analyzed. A random effects model was chosen. Continuous variables were calculated using the standardized mean difference comparing improvement from baseline of the experimental group with the control group. New vertebral fracture risk was calculated using log odds ratio. Six studies met the criteria. The pain visual analog scale (VAS) mean difference was 0.73 (confidence interval [CI] 0.35, 1.10) for early (&lt;12 weeks) and 0.58 (CI 0.19, 0.97) for late time points (6 to 12 months), favoring vertebroplasty (p &lt; 0.001). The functional outcomes at early and late time points were statistically significant with 1.08 (CI 0.33, 1.82) and 1.16 (CI 0.14, 2.18), respectively. The HRQOL showed superior results of vertebroplasty compared with conservative care at early and late time points of 0.39 (CI 0.16, 0.62) and 0.33 (CI 0.16, 0.51), respectively. Secondary fractures were not statistically different between the groups, 0.065 (CI −0.57, 0.70). This meta-analysis showed greater pain relief, functional recovery, and health-related quality of life with cement augmentation compared with controls. Cement augmentation results were significant in the early (&lt;12 weeks) and the late time points (6 to 12 months). This meta-analysis provides strong evidence in favor of cement augmentation in the treatment of symptomatic VCF fractures. © 2013 American Society for Bone and Mineral Research
PTH-enhanced structural allograft healing is associated with decreased angiopoietin-2–mediated arteriogenesis, mast cell accumulation, and fibrosis
Authors: Dhillon Robinder S , Xie Chao , Tyler Wakenda , Calvi Laura M , Awad Hani A , Zuscik Michael J , O'Keefe Regis J , Schwarz Edward M 
Published: September 2012

Abstract
Recombinant parathyroid hormone (rPTH) therapy has been evaluated for skeletal repair in animal studies and clinical trials based on its known anabolic effects, but its effects on angiogenesis and fibrosis remain poorly understood. We examined the effects of rPTH therapy on blood vessel formation and osseous integration in a murine femoral allograft model, which caused a significant increase in small vessel numbers, and decreased large vessel formation (p &lt; 0.05). Histology showed that rPTH also reduced fibrosis around the allografts to similar levels observed in live autografts, and decreased mast cells at the graft-host junction. Similar effects on vasculogenesis and fibrosis were observed in femoral allografts from Col1caPTHR transgenic mice. Gene expression profiling revealed rPTH-induced angiopoietin-1 (8-fold), while decreasing angiopoietin-2 (70-fold) at day 7 of allograft healing. Finally, we show anti-angiopoietin-2 peptibody (L1-10) treatment mimics rPTH effects on angiogenesis and fibrosis. Collectively, these findings show that intermittent rPTH treatment enhances structural allograft healing by two processes: (1) anabolic effects on new bone formation via small vessel angiogenesis, and (2) inhibition of angiopoietin-2–mediated arteriogenesis. The latter effect may function as a vascular sieve to limit mast cell access to the site of tissue repair, which decreases fibrosis around and between the fractured ends of bone. Thus, rPTH therapy may be generalizable to all forms of tissue repair that suffer from limited biointegration and excessive fibrosis. © 2013 American Society for Bone and Mineral Research.
Tooth root dentin mineralization defects in a mouse model of hypophosphatasia
Authors: Foster BL , Nagatomo KJ , Tso HW , Tran AB , Nociti FH , Narisawa S , Yadav MC , McKee MD , Millán JL , Somerman MJ 
Published: September 2012

Abstract
Tissue-nonspecific alkaline phosphatase (TNAP) is expressed in mineralizing tissues and functions to reduce pyrophosphate (PPi), a potent inhibitor of mineralization. Loss of TNAP function causes hypophosphatasia (HPP), a heritable disorder marked by increased PPi, resulting in rickets and osteomalacia. Tooth root cementum defects are well described in both HPP patients and in Alpl−/− mice, a model for infantile HPP. In Alpl−/− mice, dentin mineralization is specifically delayed in the root; however, reports from human HPP patients are variable and inconsistent regarding dentin defects. In the current study, we aimed to define the molecular basis for changes in dentinogenesis observed in Alpl−/− mice. TNAP was found to be highly expressed by mature odontoblasts, and Alpl−/− molar and incisor roots featured defective dentin mineralization, ranging from a mild delay to severely disturbed root dentinogenesis. Lack of mantle dentin mineralization was associated with disordered and dysmorphic odontoblasts having disrupted expression of marker genes osteocalcin and dentin sialophosphoprotein. The formation of, initiation of mineralization within, and rupture of matrix vesicles in Alpl−/− dentin matrix was not affected. Osteopontin (OPN), an inhibitor of mineralization that contributes to the skeletal pathology in Alpl−/− mice, was present in the generally unmineralized Alpl−/− mantle dentin at ruptured mineralizing matrix vesicles, as detected by immunohistochemistry and by immunogold labeling. However, ablating the OPN-encoding Spp1 gene in Alpl−/− mice was insufficient to rescue the dentin mineralization defect. Administration of bioengineered mineral-targeting human TNAP (ENB-0040) to Alpl−/− mice corrected defective dentin mineralization in the molar roots. These studies reveal that TNAP participates in root dentin formation and confirm that reduction of PPi during dentinogenesis is necessary for odontoblast differentiation, dentin matrix secretion, and mineralization. Furthermore, these results elucidate developmental mechanisms underlying dentin pathology in HPP patients, and begin to explain the reported variability in the dentin/pulp complex pathology in these patients. © 2013 American Society for Bone and Mineral Research
Annexin A1 mediates the anti‐adhesive effects of dexamethasone during the cell–cell interaction between the all‐trans retinoic acid‐treated acute promyelocytic leukemic cells and endothelial cells
Authors: Tsai Wen‐Hui , Lai Shu‐Lien , Li I‐Ting , Chien Hong‐Yu , Shih Chung‐Hung , Kou Yu Ru , Hsu Hui‐Chi 
Published: September 2012

AbstractAnnexin A1 (AnxA1) is an important anti‐inflammatory mediator during granulocytic differentiation in all trans‐retinoic acid (ATRA) treated acute promyelocytic leukemic (APL) cells. Dexamethasone has been used successfully to prevent complications in ATRA‐treated APL patients, although its mechanism of action is still not clear. In the present study, we have examined the effect of dexamethasone on the modulation of AnxA1 in ATRA‐APL NB4 (ATRA‐NB4) cells, ATRA‐NB4 cells‐derived microparticles (MPs) and its role during cell–cell interaction between ATRA‐NB4 cells and endothelial cells. Our results have shown that dexamethasone can inhibit the percentage of ATRA‐NB4 cells expressing surface AnxA1 and its receptor FPR2/ALX in a time‐dependent manner based on flow cytometric analysis. However, dexamethasone treatment of ATRA‐NB4 cells has no significant effect on the level of AnxA1 mRNA, the total cellular level of AnxA1 protein or the release of AnxA1 from these cells, as determined by RT‐PCR, Western blotting, and ELISA, respectively. Further studies demonstrate that dexamethasone is able to significantly inhibit the adhesion of ATRA‐NB4 cells to endothelial cells, and this anti‐adhesive effect can be inhibited if the cells were pre‐treated with a neutralizing antibody specific for AnxA1. Finally, dexamethasone also enhances the release of AnxA1‐containing MPs from ATRA‐NB4 cells which can in turn prevent the adhesion of the ATRA‐NB4 cells to endothelial cells. We conclude that biologically active AnxA1 originating from dexamethasone‐treated ATRA‐APL cells and their MPs plays an anti‐adhesive effect and this contributes to inhibit the adhesion of ATRA‐APL cell to endothelial cells. J. Cell. Biochem. 114: 551–557, 2013. © 2012 Wiley Periodicals, Inc.
Involvement of NOS3 in RA‐Induced neural differentiation of human NT2/D1 cells
Authors: Jezierski Anna , Deb‐Rinker Paromita , Sodja Caroline , Walker P. Roy , Ly Dao , Haukenfrers Julie , Sandhu Jagdeep K. , Bani‐Yaghoub Mahmud , Sikorska Marianna 
Published: September 2012

AbstractNitric oxide (NO) plays a key role in neurogenesis as a regulator of cell proliferation and differentiation. NO is synthesized from the amino acid L‐arginine by nitric oxide synthases (NOS1, NOS2, and NOS3), which are encoded by separate genes and display different tissue distributions. We used an in vitro model of RA‐induced neural differentiation of NT2 cells to examine which of the three NO‐synthesizing enzymes is involved in this process. The results revealed a transient induction of NOS3 (known as the constitutively expressed endothelial nitric oxide synthase; eNOS) during the time course of the RA treatment. The peak of gene expression and the nuclear presence of NOS3 protein coincided with cell cycle exit of NT2‐derived neuronal precursors. The subsequent analysis of cytosine methylation and histone H3 acetylation of the human NOS3 5′ regulatory sequences indicated that epigenetic modifications, especially upstream of the proximal promoter (−734 to −989, relative to exon 2 TSS at +1), were also taking place. NOS1 was expressed only in the differentiated neurons (NT2‐N), whereas NOS2 was not expressed at all in this cellular model. Thus, a burst of NO production, possibly required to inhibit neural cell proliferation, was generated by the transient expression of NOS3. This pattern of gene expression, in turn, required epigenetic remodeling of its regulatory region. Published 2012 Wiley Periodicals, Inc.
Statewide and local recruitment of mourning doves in Missouri
Authors: Schulz John H. , Bonnot Thomas W. , Mong Tony W. , Millspaugh Joshua J. 
Published: September 2012

AbstractIntensive harvests have the potential to greatly affect local mourning dove (Zenaida macroura) populations, a popular gamebird and songbird. To evaluate if recruitment was commensurate with harvest, we applied a ratio‐based method to estimate local and statewide mourning dove recruitment across 7 public hunting areas in Missouri from 2005 to 2011. We estimated recruitment from preharvest adult sex ratios and harvest age ratios that incorporated various methods to address potential inherent biases (e.g., bias in the adults of unknown sex in preharvest samples, bias in unknown age wings, and local differential vulnerability; DV). Data from 356 radio‐marked doves revealed a DV rate, where hatch year doves were, on average 2.7× more likely to be harvested than adult doves. Recruitment estimates for local areas were highly variable and in some cases, biologically unrealistic (e.g., >10 offspring/female), because of small preharvest sample sizes. However, data pooled statewide provided recruitment estimates of 3.1 offspring/female (±0.3 SE) or 4.1 offspring/female (±0.3 SE), assuming samples of unknown sex doves were female biased or male biased, respectively. Although statewide estimates agree with directly observed rates, the sex ratios and differential vulnerability comprising them vary considerably from what has been previously assumed. Whether preharvest sex ratios are biased from trapping methods has 2 important implications; either regional approaches have overestimated recruitment or the number of females in Missouri's population is much less than originally thought. Because each of these scenarios are important to understanding the effects of regional harvest management on Missouri's dove population, they highlight the importance of a better understanding of biases involved in estimating recruitment. © 2012 The Wildlife Society.
Idiopathic ulcerative laryngitis causing midmembranous vocal fold granuloma
Authors: Sinclair Catherine F. , Sulica Lucian 
Published: September 2012

AbstractIdiopathic ulcerative laryngitis (IUL) is characterized by bilateral midmembranous vocal fold ulceration, which follows upper respiratory infection with cough. In contrast, granuloma of the membranous vocal fold can occur rarely following microlaryngoscopy, presumably secondary to surgical violation of deep tissue planes. We report a novel case of noniatrogenic membranous vocal fold granulation developing in a patient with IUL. Although the presence of granulation implied injury to the entire microstructure of the vibratory portion of the vocal fold, the lesion resolved with conservative management without adverse sequelae.
Remarkable differences between three evidence‐based guidelines on management of obstructive sleep apnea‐hypopnea syndrome
Authors: Aarts Mark C. J. , van der Heijden Geert J. M. , Rovers Maroeska M. , Grolman Wilko 
Published: September 2012

AbstractObjectives/HypothesisThe aim of this study was to compare available guidelines for the diagnosis and treatment of patients with obstructive sleep apnea‐hypopnea syndrome (OSAHS) regarding their content, conclusions on the available evidence, and recommendations.Study Design:Literature review/systematic review.MethodsWe retrieved guidelines from Embase, PubMed, Web of Science, and Web sites of several health care improvement centers and with a Google Scholar search. We appraised the quality of selected guidelines according to the Appraisal of Guidelines for Research and Evaluation instrument. For similar clinical questions we compared the conclusions, the attached levels of evidence, and the references used. If differences were found, we checked search strategies, appraisal criteria, and publication date as possible sources for these differences.ResultsWe selected the guidelines on diagnosis and treatment of OSAHS of the Scottish Scottish Intercollegiate Guidelines Network, the Dutch Institute for Healthcare Improvement, and the Institute for Clinical Systems Improvement in the United States for this comparison. For similar clinical questions these three guidelines showed conflicting conclusions (11%–18%), differences in attached levels of evidence (32%–62%), and remarkable discrepancies in cited studies. A plausible reason for these differences is the citation preference for articles from members of the guidelines workgroup and from their own country. Despite different publication dates, more recent guidelines fail to cite earlier published guidelines.ConclusionsDespite the generally accepted approach regarding the development of evidence‐based guidelines, remarkable differences exist between guidelines from different countries on the same clinical subject. Laryngoscope, 2013
The influence of a vestibular dysfunction on the motor development of hearing‐impaired children
Authors: De Kegel Alexandra , Maes Leen , Baetens Tina , Dhooge Ingeborg , Van Waelvelde Hilde 
Published: September 2012

AbstractObjectives/Hypothesis:To identify the predictive ability of vestibular function test results on motor performance among hearing‐impaired children.Study Design:Cross‐sectional study.Methods:Fifty‐one typically developing children and 48 children with a unilateral (n = 9) or bilateral hearing impairment (n = 39) of more than 40 dB HL between 3 and 12 years were tested by the Movement Assessment Battery for Children–Second Edition (M ABC‐2), clinical balance tests, posturography, rotatory chair testing, and vestibular evoked myogenic potential (VEMP). From the group of hearing‐impaired children, 23 had cochlear implants.Results:Balance performance on M ABC‐2, clinical balance tests, as well as the sway velocity assessed by posturography in bipedal stance on a cushion with eyes closed and in unilateral stance differed significantly between both groups. Presence of a VEMP response is an important clinical parameter because comparison of the motor performance among hearing‐impaired children between those with present and absent VEMPs showed significant differences in balance performance. The three most important predictor variables on motor performance by bivariate regression analyses are the vestibular‐ocular reflex (VOR) gain value of the rotatory chair test at 0.01 and 0.05 Hz frequency, as well as the VEMP asymmetry ratio. Multivariate regression analyses suggest that the VOR asymmetry value of the rotatory chair test at 0.05 Hz and the etiology of the hearing loss seem to have additional predictive value.Conclusions:Hearing‐impaired children are at risk for balance deficits. A combination of rotatory chair testing and VEMP testing can predict the balance performance.
Laryngeal pathologies in older Korean adults and their association with smoking and alcohol consumption
Authors: Byeon Haewon , Lee Yunhwan 
Published: September 2012

AbstractObjectives/Hypothesis:This study's objectives were to assess the prevalence rate of laryngeal pathologies in the Korean elderly, and to examine the association of smoking and alcohol consumption with laryngeal diseases.Study Design:Cross‐sectional study.Methods:Data were from the 2008 Korea National Health and Nutritional Examination Survey. Subjects were 663 elderly persons (261 men and 402 women) between the ages of 65 and 84 years who completed the laryngoscopic examination. Weighted prevalence of laryngeal pathologies was compared by demographic characteristics, smoking, alcohol drinking, body mass index, and self‐reported voice problems. Multiple logistic regression analyses were conducted to examine the independent as well as combined influence of smoking and alcohol consumption on laryngeal lesions.Results:The prevalence of laryngeal pathologies in the Korean elderly between the ages of 65 and 84 years was 8.1%. The prevalence of laryngeal lesions increased with age and was higher among men, middle school and high school graduates, manual workers, current smokers, current alcohol drinkers, and those with self‐reported voice problems. Adjusting for covariates, current smokers, compared with nonsmokers, were more likely to have laryngeal pathologies (odds ratio [OR], 2.18; 95% confidence interval [CI], 1.01–4.67). Current alcohol drinking was not independently associated with laryngeal diseases. Concurrent smoking and alcohol drinking, however, were associated with a significantly higher risk of laryngeal pathologies (OR, 3.29; 95% CI, 1.22–8.88).Conclusions:Smoking and alcohol consumption may increase the risk of laryngeal diseases in later life.
Spectrum of immune‐mediated inner ear disease and cochlear implant results
Authors: Malik Mohammad U. , Pandian Vinciya , Masood Hamid , Diaz David A. , Varela Voss , Dávalos‐Balderas Alfredo José , Parra‐Cardenas Martha , Seo Phillip , Francis Howard W. 
Published: September 2012

AbstractObjectives/Hypothesis:To characterize the progression of hearing loss in patients with immune‐mediated inner ear disease (IMIED), and to identify disease‐ and patient‐specific factors associated with cochlear implant (CI) performance.Study Design:Retrospective cohort study.Methods:Subjects consisted of CI patients suspected to have lost their hearing due to IMIED. The primary dependent variable for functional decline was time to deafness, whereas for CI benefit it was post‐CI speech perception scores. Independent variables included presence or absence of systemic autoimmune disease, age at CI, and insertion depth of the cochlear electrode.Results:A transient favorable response to immunosuppressive therapy was reported in 16 of 26 patients (66.67%). The time to deafness differed between an organ (ear)‐specific immune‐mediated group, a systemic immune‐mediated group including Cogan syndrome and relapsing polychondritis (subgroup A), and a systemic immune‐mediated group associated with other autoimmune diseases (subgroup B; P = .001). Disease group (−15.52; P = .04), insertion depth of the CI electrode (40.71; P = .01), and the age at CI (−0.48, P = .05) were associated with speech perception results.Conclusions:Triaging IMIED cases based on presence and type of systemic autoimmune disease may aid in selecting a management strategy. Knowledge about the predictors of CI outcome will help clinicians select appropriate patients for CIs. In the setting of significant and irreversible hearing deficit, the restoration of hearing using a cochlear prosthesis may be appropriate earlier rather than later.