bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proliferating Cell Nuclear Antigen/Cyclin in Cultured Human Keratinocytes
Authors: Okada Natsuko , Miyagawa Sachiko , Steinberg Mark L. , Yoshikawa Kunihiko 
Published: April 2015

AbstractExpression of proliferating cell nuclear antigen (PCNA)/cyclin in cultured human keratinocytes was studied using an antibody from an SLE patient as the reagent. By indirect immunofluorescence staining, SV40‐transformed human keratinocytes expressed PCNA/cyclin in 40~45% of the cells as a nuclear granular fluorescence. After synchronization of these cells, their nuclear distribution pattern during the S phase was sequential and showed a clear correlation with DNA synthesis. Primary cultured keratinocytes grown in high Ca# medium expressed PCNA/cyclin in 10~15% of the cells with a similar staining pattern. These positively stained cells were confined to the basal and immediate suprabasal layers of the stratified culture sheet. The keratinocytes disaggregated by trypsin were separated according to cell size through a screen of Nitex monofilament cloth. The cells smaller than 15 µm in diameter synthesized abundant PCNA/cyclin, while the larger cells expressed very low levels. These results indicate that the expression of PCNA/cyclin correlates with DNA synthesis in cultured keratinocytes, but is not associated with their differentiation process.
Expression of Interleukin 1 in Burn Scars
Authors: Sawada Yukimasa , Takahashi Masaaki , Nomura Kazuo 
Published: April 2015

AbstractA histochemical study using a Biotin‐Streptavidin procedure for demonstrating interleukin 1 (IL‐1) in burn scar specimens is described. Among seventeen scar specimens, five positive reactions to IL‐1 were observed in the epidermis. No positive reactions in the dermal tissue, however, were detected. The twenty specimens of normal skin used as controls showed no positive reaction in either the dermis or the dermis.
A Comparative Study of Fluorescence in Malignant Melanoma and Nevocellular Nevus Using a Fluorescence Microscope and Formalin‐fixed Specimens
Authors: Shukuwa Tetsuo , Nonaka Shigeo , Yoshida Hikotaro 
Published: April 2015

AbstractFluorescence in malignant melanoma cells was investigated. The specimens from 18 cases of malignant melanoma and 26 cases of nevocellular nevus, which were fixed with formalin and embedded in paraffin wax, were studied by the fluorescence microscopic method.On the fluorescence microscope, the malignant melanoma cells emitted intense fluorescence from the cytoplasm. The nevus cells with large amounts of melanin granules showed moderate fluorescence. The tumor cells of melanoma in situ and nevus cells with few melanin granules emitted little fluorescence. Not only malignant melanoma cells but also nevus cells in the formalin fixed specimens had various degrees of fluorescence. Many cases of malignant melanoma emitted intense fluorescence, but this was rarely found in nevocellular nevus. This method is also useful in differentiating melanoma from nevocellular nevus.
Treatment of a Case of Mycosis Fungoides and One of Parapsoriasis en Plaque with Topical PUVA Using a Monofunctional Furocoumarin Derivative, 4,6,4'‐Trimethylangelicin
Authors: Morita Akimichi , Takashima Akira , Nagai Morihito , Dall'Acqua Francesco 
Published: April 2015

AbstractA case of plaque stage mycosis fungoides and one of parapsoriasis en plaque were treated with topical PUVA therapy using a monofunctional furocoumarin derivative, 4,6,4'‐trimethylangelicin (TMA). Both patients showed complete clearance of eruptions within 16 treatments. The therapeutic effectiveness of TMA was confirmed by the fact that those eruptions exposed to UVA alone, without TMA application, showed slower and less significant improvement. Histologically, dermal infiltrates of mycosis cells and associated epidermotrophism disappeared almost completely in response to TMA PUVA. No side effects or changes in values in laboratory examinations were observed during treatment.
Gold Sodium Thiomalate (GTM) Induces Hypersensitivity to Thiomalate, the Thiol Carrier of GTM
Authors: Ikezawa Zenro , Kitamura Kazuko , Nakajima Hiroshi 
Published: April 2015

AbstractA case of the gold sodium thiomalate (GTM)‐induced eruptions with thiomalate (TM) hypersensitivity was reported. A 61‐year‐old Japanese woman developed lichenoid and seborrheic dermatitis (SD)‐like eruptions with alopetia, when the total dosage of GTM administered for rheumatoid arthritis became 110 mg. The eruptions slowly disappeared with pigmentation after discontinuance of the GTM therapy, and the resumption resulted in the development of similar eruptions. She showed a positive reaction to GTM in an intradermal test. She also showed a positive response to TM, which is the thiol carrier of GTM, in the patch test, but a negative one to metallic gold. After administration of auranofin (AF), she also developed the SD‐like eruptions with hypersensitivity to metallic gold as well as AF on patch testing, but did not develop the lichenoid ones. Our animal experiments revealed an almost complete cross reaction between GTM and TM, but only a partial one between GTM and aurothioglucose, which have dissmilar structures in the carrier part for gold. Probable roles of hypersensitivity to TM and metallic gold, which are metabolites of GTM, were discussed, respectively, in the genesis of the GTM‐induced lichenoid eruptions and the AF‐induced SD‐like eruptions.
SH Abundant Body in Infantile Digital Fibromatosis
Authors: Kanamori Masashi , Hashimoto Kenji , Tanaka Hideyuki , Shimizu Masayuki 
Published: April 2015

AbstractA case of infantile digital fibromatosis was studied with regard to the distribution of ‐SH groups and SS linkages, using N‐(7‐dimethylamino‐4‐methyl‐3‐coumarinyl) maleimide (DACM) stain. Tumor cells showed a strong fluorescence in the cytoplasm from the ‐SH stain. A brilliant ‐SH fluorescence was also homogenously present on the intracytoplasmic inclusions. SS reactivity was negative. Although further studies should be necessary to ascertain this finding, the DACM stain may make a simple and objective diagnostic method of infantile digital fibromatosis.
Dermal Nerve Sheath Myxoma
Authors: Yamashita Noriko , Minami Shoichiro , Yu Masaaki , Miyazaki Takao , Sagami Seiichiro 
Published: April 2015

AbstractA case of dermal nerve sheath myxoma on the left palm of 26‐year‐old Japanese man is presented. We examined this tumor by light and electron microscopies. On light microscopic examination, this tumor was characterized by a multilobulated myxoid tumor composed of stellate cells in an abundant mucous matrix. This histological feature resembled previously reported cases of dermal nerve sheath myxoma. On electron microscopic examination, the findings of these tumor cells were similar to those of the perineurial cells. These microscopical examinations have provided evidence for an origin from perineurial cells rather than Schwann cells.
Intradermal Spitz Nevus Differentiated from Reticulohistiocytic Granuloma by Immunoreactivity to S‐100 Protein
Authors: Kato Naoko , Ueno Hiroo 
Published: April 2015

AbstractA firm, dark reddish, smooth surfaced nodule on the forearm of a 16‐year‐old boy was diagnosed as a Spitz nevus with the aid of a strong positive reactivity to S‐100 protein. Histological examination revealed an intradermal epithelioid cell tumor with prominent multinucleated giant cells, suggesting the diagnosis of reticulohistiocytic granuloma. However, immunoperoxidase staining of the tumor cells showed strong positive reactivity to S‐100 protein and vimentin; it was negative for lysozyme and alpha‐1 antitrypsin. Although a few melanosomes in the tumor cells seen in electron microscopic examination provided crucial proof for the diagnosis of Spitz nevus, the positive reactivity to S‐100 protein in ordinary formalin‐fixed, paraffin‐embedded tissues proved to be very useful in the differentiation of Spitz nevus from tumors of histiocytic origin, especially those of the mononuclear phagocytic system.
A Possible Pathogenesis for Blackfoot Disease
Authors: Chen Gwo‐Shing , Asai Toshiya , Suzuki Yuhsuke , Nishioka Kiyoshi , Nishiyama Shigeo 
Published: April 2015

AbstractBlackfoot disease (BFD) is an endemic peripheral vascular occlusive disease found among the inhabitants of the southwest coast of Taiwan. The clinical features of BFD are similar to those of Buerger's disease. Pathology shows arteriosclerosis obliterans and thromboangiitis obliterans. The high arsenic content of artesian well water in the area is regarded as the main causal factor of this disease. Therefore, the purpose of this study was to observe the toxic effects of various arsenic concentrations on cultured human umbilical vein endothelial cells (HUV‐EC). The methods of this study included cell growth assay, 51Cr‐release assay, and staining of Factor VIII related antigen (FVIII‐RAg) and Ulex europaeus agglutinin I (UEA‐I) binding sites of HUV‐EC. The following data were obtained: 1) no obvious cytotoxicity in 51Cr‐release assay; 2) inhibition of the synthesis of both FVIII‐RAg and UEA‐I binding sites when the arsenic concentration was above 100 ng/ml; 3) dose‐dependent inhibition of growth of HUV‐EC by any concentration of arsenic. At a higher concentration of more than 100 ng/ml, arsenic inhibited endothelial cell proliferation and glycoprotein synthesis, whereas it only inhibited the proliferation at a lower concentration of less than 50 ng/ml. It is suggested that arsenic, at both higher and lower concentrations, may damage endothelial cells. Such damage may play an important role in the pathogenesis of BFD.
Cutaneous Involvement as a Presenting Feature of Monocytic Leukemia: Morphological and Immunohistochemical Studies
Authors: Watanabe Shinichi , Fujimura Mami , Kashima Masato , Mizoguchi Masako , Takahashi Hisashi , Fujita Akira 
Published: April 2015

AbstractThe clinical and pathological findings in a patient with monocytic aleukemic leukemia presenting initially as multiple monoblastic tumors of the skin is described. The patient was a 35‐year‐old Japanese woman, who had first noticed multiple, asymptomatic, reddish‐brown papules on her trunk. Asymptomatic enlargements of several lymph nodes were present in the bilateral cervical and axillary areas. There was no hepatosplenomegaly, sternal tenderness, bruising, or bleeding. The skin and lymph node biopsies were originally interpreted as malignant lymphoma. The diagnosis of acute monocytic leukemia was established when bone marrow involvement was detected.Immunohistochemical observation of the skin eruptions revealed the following: Positive staining with lysozyme was noted in almost half of the infiltrating atypical cells. Most of the infiltrating cells reacted positively with antisera to Leu‐M5 and some of them reacted to Leu‐M1. The helper T cell antibody, Leu‐3a+3b, showed weak positive staining of most infiltrating cells. However, there were no reactions with antisera to Leu‐6, Leu‐7, Leu‐14, CALLA, OKT 6, OKT 8, OKT 16, OKB 19, OKM 14, βF1, or δTCS1. OKM 5‐positive keratinocytes were observed in some parts of the upper epidermis, although no OKM 5 expression could be detected on any tumor cells.Cytochemistry, immunohistochemistry, and electron microscopy can aid in the diagnosis of monocytic leukemia. This case illustrates the importance of using an expanded panel of monoclonal antisera in certain hematopoietic tumors.
Laboratory Observations of Haemaphysalis longicornis Neumann after a Human Bite
Authors: Maeoka Yoshiaki , Baba Shunichi , Suzuki Hiroyuki 
Published: April 2015

AbstractA case of a tick bite with Haemaphysalis longicornis Neumann is reported. The tick was found on the left lower abdomen of a 62‐year‐old female. The tick bite occurred in Fuchu city, a suburb of Tokyo. This paper presents laboratory observations of the process of Haemaphysalis longicornis Neumann laying eggs and becoming larvae after biting a human. The results of our observations include the size of female adult (length: 7.5 mm, width: 5.2 mm, weight: 96 mg) after blood sucking, preoviposition period (7 days), oviposition period (24 days), number of eggs (760 eggs), egg size (length: 0.58~0.62 mm, width: 0.39~0.42 mm), size of unfed larva (length: 0.45~0.5 mm, width: 0.45~0.5 mm) and unfed larva period until death, which ranged from 10 to 62 days.
Subclass Characteristics of IgG Autoantibodies in Bullous Pemphigoid and Pemphigus
Authors: Shirakata Yuji , Shiraishi Satoshi , Sayama Koji , Miki Yoshiharu 
Published: April 2015

AbstractImmunoglobulin (Ig) G subclasses in anti‐basement membrane zone (BMZ) autoantibodies found in the sera of bullous pemphigoid (BP) and in anti‐intercellular substance (ICS) autoantibodies of pemphigus were investigated using immunofluorescent (IF) staining. In BP, IgG4, IgG1, and IgG2 were detected in 13, 5 and 6 of 15 patients, respectively; IgG3 was not detected. In pemphigus, IgG4 was detected in all of 10 patients, IgG1 in 7, IgG2 in one, and IgG3 in one patient, respectively. In both BP and pemphigus, the most prominent subclass in intensity of IF staining was IgG4. Although one BP and one PV patient had only IgG4 autoantibodies, C3 deposition was detected.The quantification of IgG subclasses in the sera of the patients was performed by enzymelinked immunosorbent assays (ELISA). Serum levels of IgG4 in both BP and pemphigus were elevated approximately 3‐fold over those in normal controls; those of whole IgG and IgG1–3 were not significantly elevated. Using direct IF staining, the deposition of C3 at the BMZ and at the ICS was demonstrated in 9 of 10 BP and in 3 of 8 pemphigus patients, respectively.The prominent IgG subclasses of anti‐BMZ and anti‐ICS antibody were IgG4, a non‐complement‐fixing antibody, suggesting that the deposition of C3 in the lesional skin occurred via the alternative pathway, or that small amounts of IgG1–3 subclass autoantibodies activated the classical pathway.
Co‐culture of Human Hair Follicles and Dermal Papillae in a Collagen Matrix
Authors: Arase Seiji , Sadamoto Yasushi , Katoh Shouji , Urano Yoshio , Takeda Katsuyuki 
Published: April 2015

AbstractHuman hair follicles, either alone or in combination with dermal papillae, were cultured in a collagen matrix. When plucked hair follicles were cultured alone, spike‐like structures composed of outer root sheath cells started growing around the follicle and then radiated into the gel. When isolated dermal papillae were embedded close to the follicles, spikes started growing earlier and grew more rapidly than without the papillae. In cultures of excised follicles from which the dermal papilla had been removed, epithelial cells (possibly hair bulb cells) started growing out from the bulbous portion and then also formed spikes. In the presence of a papilla, the spikes elongated toward the papilla, finally reaching and surrounding it. These findings suggest that dermal papilla cells produce a factor(s) that enhances growth of follicular epithelial cells and also attracts those cells.In cultures of whole excised follicles, two major characteristic patterns of cellular growth were recognized. When the dermal papilla remained inside the bulb in contact with the hair bulb matrix, the hair matrix cells proliferated and differentiated in the normal manner, resulting in elongation of the hair shaft and follicle. But when the papilla was detached from the hair bulb matrix, epithelial cells proliferated from the bulbous portion and finally formed hair follicle‐like structures. Thus, attachment of the dermal papilla to the hair bulb matrix in the bulbous portion appears to be necessary for growth of the hair and follicle in the normal manner.Our model may be useful for examining the interaction between follicular epithelial cells and dermal papillae and for studying the growth of hair and follicles in vitro.
Psoriasiform Intradermal Test Reaction to ABPC in a Patient with Psoriasis and ABPC Allergy
Authors: Saito Sumi , Ikezawa Zenro 
Published: April 2015

AbstractWe experienced a case of psoriasis with ABPC allergy which showed a psoriasiform intradermal test reaction to ABPC. A 22‐year‐old man with a history of psoriasis since the age of 17 was admitted to our department because he developed erythrodermic psoriasis after oral administration of ABPC. About 1 year later, he again developed erythroderma after administration of ABPC. Intradermal testing with 2% ABPC was performed. We found infiltrating erythema at day 2, followed by a gradually increasing psoriasiform reaction at 6 days on the site of ABPC skin test. We examined the infiltrating cells in the skin test reaction immunohistochemically. Most of the infiltrating cells in the dermis were activated helper/DTH‐type T cells. Among the infiltrating cells in the epidermis, we observed more cytotoxic/suppressor‐type T cells than helper/DTH‐type T cells. A possible role of a DH reaction to ABPC in inducing the psoriatic lesion is discussed in relation to the pathomechanisms of psoriasis.
Epidermolysis Bullosa Atrophicans Generalisata Mitis
Authors: Yamada Yoshitaka , Dekio Satoshi , Jidoi Joji , Ishimoto Takako , Yoshioka Takafumi 
Published: April 2015

AbstractA male adult epidermolysis bullosa atrophicans generalisata mitis (EBAGM) patient with renal dysfunction is described. The abnormal urological findings were improved together and healing of the generalized skin erosions occurred. To our best knowledge, he is the 3rd EBAGM patient with urological abnormalities in the world.
Pleomorphic Adenoma of the Lip
Authors: Narita Hajime , Kobayashi Taisuke , Kanzaki Tamotsu 
Published: April 2015

AbstractA case of pleomorphic adenoma in the upper lip is reported. A 61‐year‐old male had a tumor at the left side of the upper lip for 8 years. The tumor was surgically excised without recurrence. Histopathologically, the tumor was a typical pleomorphic adenoma with keratinization and glandular differentiation of tumor cells and fibroblastic, mucinous, hyalinized, and cartilagenous stroma.
Treatment of Cutaneous T‐cell Lymphomas (CTCL) with Extracorporeal Photochemotherapy
Authors: Nagatani Tetsuo , Matsuzaki Toshiko , Kim Shu‐taku , Baba Naoko , Osawa Junko , Sugiyama Asami , Komatsu Hitoshi , Ichiyama Shinichi , Takahashi Yasuhide , Miyamoto Hideaki , Nakajima Hiroshi , Shimoda Nagayoshi 
Published: April 2015

AbstractSince August of 1988, we have treated seven CTCL patients with extracorporeal photochemotherapy, including two with tumor‐stage mycosis fungoides (MF) showing mucinous degeneration, two with plaque‐stage MF, and two with erythrodermatous MF. One was withdrawn just after the first trial. For each patient, the phenotypes of peripheral blood lymphocytes were analyzed by flow cytometry in terms of the percentages of OKT11, OKT3, OKT4, Leu9, OKT8, B1, Tac, OKT9, OKIal, Leu7, Leu3a/4B4, and Leu3a/2H4 cells. These parameters were compared with the clinical responses according to skin score. The two patients with tumors died, but the five patients without tumors did not. Three of the 6 patients responded to the treatment. Side effects that are often associated with standard chemotherapy, such as bone marrow suppression, gastrointestinal symptoms and hair loss, were not observed. One cardiovascular event (1 patient) occurred. No significant changes in T‐cell subsets were seen during the course of therapy. These preliminary data suggest that extracorporeal photochemotherapy may be effective for CTCL other than tumor‐stage MF.
Poikiloderma‐like Lesions on the Neck in Atopic Dermatitis: A Histopathological Study
Authors: Mukai Hideki , Nishioka Kiyoshi , Kamimura Kimio , Katayama Ichiro , Nishiyama Shigeo 
Published: April 2015

AbstractReticulate pigmentation with or without skin atrophy, depigmentation and telangiectasia is frequently encountered on the neck of severe cases of adult type atopic dermatitis. These skin changes were graded clinically into 3 stages. Based on histological features, hyperplasia of the sebaceous gland, dilated tortuous capillaries, and mild degeneration of elastic fibers were noted in stage I lesions. Lesions of both stages II and III contained increased melanin in the basal cell layer with incontinence of pigment, remarkable destruction and degeneration of elastic fibers, proliferated and dilated capillaries, and deposition of mucinous substances. The numbers of mast cells in papillary dermis were significantly increased in late stage I and stage II lesions. Poikiloderma‐like lesions on the neck could be attributable to chronic inflammation and delay of wound healing process, possibly caused by long‐standing topical corticosteroid therapy.
Topical PUVA, Etretinate, and Combined PUVA and Etretinate for Palmoplantar Pustulosis: Comparison of Therapeutic Efficacy and the Influences of Tonsillar and Dental Focal Infections
Authors: Matsunami Eriko , Takashima Akira , Mizuno Nobuyuki , Jinno Takuzo , Ito Hirotaka 
Published: April 2015

AbstractTwenty patients with palmoplantar pustulosis (PPP) were treated with topical PUVA, oral etretinate (Re), or combined PUVA and etretinate (Re‐PUVA). Re and Re‐PUVA treated sites improved and/or cleared more rapidly than PUVA treated sites. Complete clearance was observed in six of ten sites treated with Re‐PUVA, two of ten with Re, and one of ten sites with PUVA within 12 weeks. UVA‐control sites failed to be cleared within 12 weeks. Remission periods after stopping the treatment were 1.5 ± 0.5 weeks (n=2) with Re, 10.5 ± 11.4 weeks (n=6) with Re‐PUVA, and one year (n=1) with PUVA. These results overall suggested that Re‐PUVA is the most effective treatment for PPP.Tonsillar focal infection (TFI) and dental focal infection (DFI) were found in 6/20 and 17/20 patients, respectively. However, the presence of focal infection (FI), TFI and/or DFI, did not appear to interfere with the therapeutic activities of Re and/or PUVA, because the complete clearance rates and remission periods in FI(+) patients were comparable with those in FI(—) patients.
A Case of Sclerosing Lymphangitis of the Lip
Authors: Inamura Kei‐ichi , Suzuki Yuhsuke , Nishioka Kiyoshi , Nishiyama Shigeo 
Published: April 2015

AbstractA 62‐year‐old woman noted a cord‐like swelling on the inner surface of her upper lip. A cross‐section of the lesion showed a radial‐shaped lumen surrounded by a thickened fibrous wall. Immunohistochemical staining with the anti‐factor VIII‐related antigen (FVIII‐RAg) antibody was negative on the luminal surface and swollen wall, although the vasa vasorum in the swollen wall were positive. The lesion was thus considered to be of lymphatic origin; that is, it derived from a lymphatic collecting vessel. For the present case, the term ‘lymphangiopathia obliterans’ is considered appropriate. This is the second report of such a lesion appearing on the lip in the literature.
Mal de Meleda‐like Palmoplantar Keratoderma
Authors: Iio Tomoe , Shiraishi Satoshi , Sayama Koji , Miki Yoshiharu 
Published: April 2015

AbstractA 38‐year‐old Japanese man was seen for severe palmoplantar keratoderma, extending to the dorsal aspects with red rims. He had had spontaneous amputations of the toes and surgical amputation of the right lower leg because of squamous cell carcinoma of the right sole. The clinical symptoms suggested those of mal de Meleda, except for the absence of consanguinity and of granular layers in the epidermis. The keratoderma improved with oral etretinate treatment.
Fixed Drug Eruption Due to Pipemidic Acid
Authors: Miyagawa Sachiko , Yamashina Yukio , Hirota Sachiko , Shirai Toshihiko 
Published: April 2015

AbstractA 71‐year‐old woman with fixed drug eruption caused by pipemidic acid is reported. The patient developed typical lesions on rechallenge with pipemidic acid, but not with nalidixic acid, an analogue of pipemidic acid.