bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of a nutrition award scheme on the food and nutrient intakes of 2- to 4-year-olds attending long day care
Authors: Bell Lucinda K , Hendrie Gilly A , Hartley Jo , Golley Rebecca K 
Published: January 2015

AbstractObjectiveEarly childhood settings are promising avenues to intervene to improve children’s nutrition. Previous research has shown that a nutrition award scheme, Start Right – Eat Right (SRER), improves long day care centre policies, menus and eating environments. Whether this translates into improvements in children’s dietary intake is unknown. The present study aimed to determine whether SRER improves children’s food and nutrient intakes.DesignPre–post cohort study.SettingTwenty long day care centres in metropolitan Adelaide, South Australia, Australia.SubjectsChildren aged 2–4 years (n 236 at baseline, n 232 at follow-up).MethodsDietary intake (morning tea, lunch, afternoon tea) was assessed pre- and post-SRER implementation using the plate wastage method. Centre nutrition policies, menus and environments were evaluated as measures of intervention fidelity. Comparisons between baseline and follow-up were made using t tests.ResultsAt follow-up, 80 % of centres were fully compliant with the SRER award criteria, indicating high scheme implementation and adoption. Intake increased for all core food groups (range: 0·2–0·4 servings/d, P<0·001) except for vegetable intake. Energy intake increased and improvements in intakes of eleven out of the nineteen nutrients evaluated were observed.ConclusionsSRER is effective in improving children’s food and nutrient intakes at a critical time point when dietary habits and preferences are established and can inform future public health nutrition interventions in this setting.
The Composer as ‘Good European’: Musical Modernism,Amor fatiand the Cosmopolitanism of Frederick Delius
Authors: COLLINS SARAH 
Published: January 2015

AbstractThis article argues that early twentieth-century debates about both musical modernism and the idea of Europe were conditioned by prevailing attitudes towards autonomy. It will challenge the current rendering of modernist autonomy as depoliticized by showing how the attribution of ‘cosmopolitan’ characteristics to the music and persona of Frederick Delius indicated both an absence of affiliation and a definitive marker of Englishness. Underpinning this argument is the idea that attending to the dialectical interplay between independence and cooperation in the notion of ‘rooted cosmopolitanism’ can offer a model for a renewed conception of autonomy and commitment in musical modernism. Delius’s devotion to the writings of Friedrich Nietzsche, and Nietzsche's own analysis of European nihilism, will act as the backdrop to this discussion and help to suggest how both ‘Europe’ and musical modernism can be understood – via the notion of cosmopolitanism – as dispositions extending beyond their conventional geographical and historical demarcations.
The Genealogy of a Song: Lady Gaga's Musical Intertexts on The Fame Monster (2009)
Authors: BURNS LORI , WOODS ALYSSA , LAFRANCE MARC 
Published: January 2015

AbstractLady Gaga's work is noted for her references to the work of other artists and is often criticized for borrowed content that is perceived as exceeding mere generic norms. In this study, we engage closely with the musical and lyrical content of two songs from her extended play release, The Fame Monster (2009), with the goal of identifying and interpreting the intertextual pathways that led to these works. Relying upon theories of intertextuality, we unearth a network of texts invoked in each of her songs and consider these connections to comprise a musical genealogy, invoking Foucault's conception of genealogy as the process of revealing historical knowledge.
Blood protein polymorphism and genetic diversity in locally adapted Muscovy duck (Cairina moschata) in Nigeria
Authors: Oguntunji A.O. , Ayorinde K.L. 
Published: January 2015

SummaryCharacterization and genetic diversities among members of a species are fundamental to their improvement and conservation. This study was conducted to characterize and estimate genetic diversity in the ecotypes of the locally adapted Muscovy duck in Nigeria using blood proteins (haemoglobin, transferrin and albumin) and enzyme (carbonic anhydrase) markers. Blood samples collected from 20, 40 and 20 adult Muscovy ducks sampled randomly from the Rain Forest, Derived Savanna and Guinea Savanna ecotypes, respectively, were typed for blood proteins and enzyme polymorphism with cellulose acetate paper. Genetic variability in the studied population was accessed using heterozygosity (H), effective number of allele (ne) and polymorphism (percent P). All the 13 allelic variants expressed at the four loci were expressed in the Derived Savanna ecotype but 12 each in the Rain Forest and Guinea Savanna ecotypes. Results showed that all the four loci were polymorphic (100 percent) and the estimated heterozygosity among them was similar (0.424–0.481). Similarity in the estimated genetic variability parameters among ecotypes indicate that the sub-populations understudy was under similar evolutionary forces and there were no appreciable differences among them.
New loci and coding variants confer risk for age-related macular degeneration in East Asians
Authors: Cheng Ching-Yu , Yamashiro Kenji , Jia Chen Li , Ahn Jeeyun , Huang Lulin , Huang Lvzhen , Cheung Chui Ming G. , Miyake Masahiro , Cackett Peter D. , Yeo Ian Y. , Laude Augustinus , Mathur Ranjana , Pang Junxiong , Sim Kar Seng , Koh Adrian H. , Chen Peng , Lee Shu Yen , Wong Doric , Chan Choi Mun , Loh Boon Kwang , Sun Yaoyao , Davila Sonia , Nakata Isao , Nakanishi Hideo , Akagi-Kurashige Yumiko , Gotoh Norimoto , Tsujikawa Akitaka , Matsuda Fumihiko , Mori Keisuke , Yoneya Shin , Sakurada Yoichi , Iijima Hiroyuki , Iida Tomohiro , Honda Shigeru , Lai Timothy Yuk Yau , Tam Pancy Oi Sin , Chen Haoyu , Tang Shibo , Ding Xiaoyan , Wen Feng , Lu Fang , Zhang Xiongze , Shi Yi , Zhao Peiquan , Zhao Bowen , Sang Jinghong , Gong Bo , Dorajoo Rajkumar , Yuan Jian-Min , Koh Woon-Puay , van Dam Rob M. , Friedlander Yechiel , Lin Ying , Hibberd Martin L. , Foo Jia Nee , Wang Ningli , Wong Chang Hua , Tan Gavin S. , Park Sang Jun , Bhargava Mayuri , Gopal Lingam , Naing Thet , Liao Jiemin , Guan Ong Peng , Mitchell Paul , Zhou Peng , Xie Xuefeng , Liang Jinlong , Mei Junpu , Jin Xin , Saw Seang-Mei , Ozaki Mineo , Mizoguchi Takanori , Kurimoto Yasuo , Woo Se Joon , Chung Hum , Yu Hyeong-Gon , Shin Joo Young , Park Dong Ho , Kim In Taek , Chang Woohyok , Sagong Min , Lee Sang-Joon , Kim Hyun Woong , Lee Ji Eun , Li Yi , Liu Jianjun , Teo Yik Ying , Heng Chew Kiat , Lim Tock Han , Yang Suk-Kyun , Song Kyuyoung , Vithana Eranga N. , Aung Tin , Bei Jin Xin , Zeng Yi Xin , Tai E. Shyong , Li Xiao Xin , Yang Zhenglin , Park Kyu-Hyung , Pang Chi Pui , Yoshimura Nagahisa , Yin Wong Tien , Khor Chiea Chuen 
Published: January 2015

AbstractAge-related macular degeneration (AMD) is a major cause of blindness, but presents differently in Europeans and Asians. Here, we perform a genome-wide and exome-wide association study on 2,119 patients with exudative AMD and 5,691 controls, with independent replication in 4,226 patients and 10,289 controls, all of East Asian descent, as part of The Genetics of AMD in Asians (GAMA) Consortium. We find a strong association between CETP Asp442Gly (rs2303790), an East Asian-specific mutation, and increased risk of AMD (odds ratio (OR)=1.70, P=5.60 × 10−22). The AMD risk allele (442Gly), known to protect from coronary heart disease, increases HDL cholesterol levels by 0.17 mmol l−1 (P=5.82 × 10−21) in East Asians (n=7,102). We also identify three novel AMD loci: C6orf223 Ala231Ala (OR=0.78, P=6.19 × 10−18), SLC44A4 Asp47Val (OR=1.27, P=1.08 × 10−11) and FGD6 Gln257Arg (OR=0.87, P=2.85 × 10−8). Our findings suggest that some of the genetic loci conferring AMD susceptibility in East Asians are shared with Europeans, yet AMD in East Asians may also have a distinct genetic signature.
Draft genome sequence of adzuki bean, Vigna angularis
Authors: Kang Yang Jae , Satyawan Dani , Shim Sangrea , Lee Taeyoung , Lee Jayern , Hwang Won Joo , Kim Sue K. , Lestari Puji , Laosatit Kularb , Kim Kil Hyun , Ha Tae Joung , Chitikineni Annapurna , Kim Moon Young , Ko Jong-Min , Gwag Jae-Gyun , Moon Jung-Kyung , Lee Yeong-Ho , Park Beom-Seok , Varshney Rajeev K. , Lee Suk-Ha 
Published: January 2015

AbstractAdzuki bean (Vigna angularis var. angularis) is a dietary legume crop in East Asia. The presumed progenitor (Vigna angularis var. nipponensis) is widely found in East Asia, suggesting speciation and domestication in these temperate climate regions. Here, we report a draft genome sequence of adzuki bean. The genome assembly covers 75% of the estimated genome and was mapped to 11 pseudo-chromosomes. Gene prediction revealed 26,857 high confidence protein-coding genes evidenced by RNAseq of different tissues. Comparative gene expression analysis with V. radiata showed that the tissue specificity of orthologous genes was highly conserved. Additional re-sequencing of wild adzuki bean, V. angularis var. nipponensis and V. nepalensis, was performed to analyze the variations between cultivated and wild adzuki bean. The determined divergence time of adzuki bean and the wild species predated archaeology-based domestication time. The present genome assembly will accelerate the genomics-assisted breeding of adzuki bean.
Fabrication of high-Q lithium niobate microresonators using femtosecond laser micromachining
Authors: Lin Jintian , Xu Yingxin , Fang Zhiwei , Wang Min , Song Jiangxin , Wang Nengwen , Qiao Lingling , Fang Wei , Cheng Ya 
Published: January 2015

AbstractWe report on fabrication of high-Q lithium niobate (LN) whispering-gallery-mode (WGM) microresonators suspended on silica pedestals by femtosecond laser direct writing followed by focused ion beam (FIB) milling. The micrometer-scale (diameter ~82 μm) LN resonator possesses a Q factor of ~2.5 × 105 around 1550 nm wavelength. The combination of femtosecond laser direct writing with FIB enables high-efficiency, high-precision nanofabrication of high-Q crystalline microresonators.
M‐sequence‐based ultra‐wideband sensor network for vitality monitoring of elders at home
Authors: Sachs Jürgen , Herrmann Ralf 
Published: January 2015

The western societies are increasingly faced with the challenges of enabling a self‐determined and safe life for a growing number of elderly people. Remote monitoring of vitality is an important tool for health risk mitigation but many of the current methods either deliver only infrequent information or require conscious cooperation of the senior. The authors propose an ultra‐wideband (UWB) radio sensor network based on M‐sequence technology specifically designed for the vitality monitoring of persons living alone. It uses the frequency band in the range from 6 to 8.5 GHz (electronic communications committee (ECC)‐band), and provides precise localisation, captures breathing motion during rests, can be used for fall detection and allows the extraction of gait features. The sensor network can be installed in common living spaces. It operates continuously in the background without any user interaction. Vitality monitoring by radar sensors is based on motion detection and tracking. This poses some challenges to radar devices because of the harsh multi‐path conditions in apartments. This study discusses these challenges, illustrates how to meet them and gives examples for vitality features which can be extracted from UWB‐radar data.
Treatment of Hypertensive Patients With Diabetes and Microalbuminuria With Combination Indapamide SR/Amlodipine: Retrospective Analysis of NESTOR
Authors: Hanon Olivier , Boully Clemence , Caillard Laure , Labourée Florian , Cochiello Sophie , Chaussade Edouard 
Published: January 2015

BACKGROUND
Combination treatments for hypertension most often include a renin–angiotensin–aldosterone system (RAAS) inhibitor. However, systolic blood pressure (SBP) remains difficult to control. Non-RAAS-inhibiting strategies such as calcium channel blocker/thiazide-like diuretic combinations may offer effective alternatives.

METHODS
Hypertensive diabetic patients with microalbuminuria were included in this retrospective, post-hoc analysis of the Natrilix SR Versus Enalapril Study in Hypertensive Type 2 Diabetics With MicrOalbuminuRia (NESTOR) trial if they were uncontrolled on monotherapy (indapamide slow release (SR) 1.5mg or enalapril 10mg) and had been given add-on amlodipine 5mg. Patients uncontrolled with monotherapy/amlodipine 5mg were uptitrated to 10mg.

RESULTS
After 52 weeks, supine SBP/diastolic BP (DBP) decreased from baseline by 26±13/14±9mm Hg in the indapamide SR/amlodipine group (n = 135) and by 21±14/11±9mm Hg in the enalapril/amlodipine group (n = 156) (P = 0.006 for ΔSBP). In the amlodipine 10mg subgroup, SBP/DBP decreased from baseline by 26±13/13±9mm Hg in the indapamide SR/amlodipine group (n = 62) and by 20±13/12±8mm Hg in the enalapril/amlodipine group (n = 77) (P = 0.02 for ΔSBP). Treatment with indapamide SR/amlodipine was well tolerated. Few patients experienced edema, with no between-group differences. As expected with diuretics, slight changes in kalemia and in uricemia were observed in the indapamide SR/amlodipine group. Changes in fasting glucose, lipids, natremia, and creatinine clearance were similar between groups.

CONCLUSIONS
Indapamide SR/amlodipine results in superior SBP reduction with a safety profile in line with that of its components and tolerability equivalent to that of an angiotensin-converting enzyme inhibitor/amlodipine strategy.
Human regulator of telomere elongation helicase 1 (RTEL1) is required for the nuclear and cytoplasmic trafficking of pre-U2 RNA
Authors: Schertzer Michael , Jouravleva Karina , Perderiset Mylene , Dingli Florent , Loew Damarys , Le Guen Tangui , Bardoni Barbara , de Villartay Jean-Pierre , Revy Patrick , Londoño-Vallejo Arturo 
Published: January 2015

Abstract
Hoyeraal-Hreidarsson syndrome (HHS) is a severe form of Dyskeratosis congenita characterized by developmental defects, bone marrow failure and immunodeficiency and has been associated with telomere dysfunction. Recently, mutations in Regulator of Telomere ELongation helicase 1 (RTEL1), a helicase first identified in Mus musculus as being responsible for the maintenance of long telomeres, have been identified in several HHS patients. Here we show that RTEL1 is required for the export and the correct cytoplasmic trafficking of the small nuclear (sn) RNA pre-U2, a component of the major spliceosome complex. RTEL1-HHS cells show abnormal subcellular partitioning of pre-U2, defects in the recycling of ribonucleotide proteins (RNP) in the cytoplasm and splicing defects. While most of these phenotypes can be suppressed by re-expressing the wild-type protein in RTEL1-HHS cells, expression of RTEL1 mutated variants in immortalized cells provokes cytoplasmic mislocalizations of pre-U2 and other RNP components, as well as splicing defects, thus phenocopying RTEL1-HHS cellular defects. Strikingly, expression of a cytoplasmic form of RTEL1 is sufficient to correct RNP mislocalizations both in RTEL1–HHS cells and in cells expressing nuclear mutated forms of RTEL1. This work unravels completely unanticipated roles for RTEL1 in RNP trafficking and strongly suggests that defects in RNP biogenesis pathways contribute to the pathology of HHS.
Detecting riboSNitches with RNA folding algorithms: a genome-wide benchmark
Authors: Corley Meredith , Solem Amanda , Qu Kun , Chang Howard Y. , Laederach Alain 
Published: January 2015

Abstract
Ribonucleic acid (RNA) secondary structure prediction continues to be a significant challenge, in particular when attempting to model sequences with less rigidly defined structures, such as messenger and non-coding RNAs. Crucial to interpreting RNA structures as they pertain to individual phenotypes is the ability to detect RNAs with large structural disparities caused by a single nucleotide variant (SNV) or riboSNitches. A recently published human genome-wide parallel analysis of RNA structure (PARS) study identified a large number of riboSNitches as well as non-riboSNitches, providing an unprecedented set of RNA sequences against which to benchmark structure prediction algorithms. Here we evaluate 11 different RNA folding algorithms’ riboSNitch prediction performance on these data. We find that recent algorithms designed specifically to predict the effects of SNVs on RNA structure, in particular remuRNA, RNAsnp and SNPfold, perform best on the most rigorously validated subsets of the benchmark data. In addition, our benchmark indicates that general structure prediction algorithms (e.g. RNAfold and RNAstructure) have overall better performance if base pairing probabilities are considered rather than minimum free energy calculations. Although overall aggregate algorithmic performance on the full set of riboSNitches is relatively low, significant improvement is possible if the highest confidence predictions are evaluated independently.
Structural insight into how the human helicase subunit MCM2 may act as a histone chaperone together with ASF1 at the replication fork
Authors: Richet Nicolas , Liu Danni , Legrand Pierre , Velours Christophe , Corpet Armelle , Gaubert Albane , Bakail May , Moal-Raisin Gwenaelle , Guerois Raphael , Compper Christel , Besle Arthur , Guichard Berengère , Almouzni Genevieve , Ochsenbein Françoise 
Published: January 2015

Abstract
MCM2 is a subunit of the replicative helicase machinery shown to interact with histones H3 and H4 during the replication process through its N-terminal domain. During replication, this interaction has been proposed to assist disassembly and assembly of nucleosomes on DNA. However, how this interaction participates in crosstalk with histone chaperones at the replication fork remains to be elucidated. Here, we solved the crystal structure of the ternary complex between the histone-binding domain of Mcm2 and the histones H3-H4 at 2.9 Å resolution. Histones H3 and H4 assemble as a tetramer in the crystal structure, but MCM2 interacts only with a single molecule of H3-H4. The latter interaction exploits binding surfaces that contact either DNA or H2B when H3-H4 dimers are incorporated in the nucleosome core particle. Upon binding of the ternary complex with the histone chaperone ASF1, the histone tetramer dissociates and both MCM2 and ASF1 interact simultaneously with the histones forming a 1:1:1:1 heteromeric complex. Thermodynamic analysis of the quaternary complex together with structural modeling support that ASF1 and MCM2 could form a chaperoning module for histones H3 and H4 protecting them from promiscuous interactions. This suggests an additional function for MCM2 outside its helicase function as a proper histone chaperone connected to the replication pathway.
Functional regulation of the DNA damage-recognition factor DDB2 by ubiquitination and interaction with xeroderma pigmentosum group C protein
Authors: Matsumoto Syota , Fischer Eric S. , Yasuda Takeshi , Dohmae Naoshi , Iwai Shigenori , Mori Toshio , Nishi Ryotaro , Yoshino Ken-ichi , Sakai Wataru , Hanaoka Fumio , Thomä Nicolas H. , Sugasawa Kaoru 
Published: January 2015

Abstract
In mammalian nucleotide excision repair, the DDB1–DDB2 complex recognizes UV-induced DNA photolesions and facilitates recruitment of the XPC complex. Upon binding to damaged DNA, the Cullin 4 ubiquitin ligase associated with DDB1–DDB2 is activated and ubiquitinates DDB2 and XPC. The structurally disordered N-terminal tail of DDB2 contains seven lysines identified as major sites for ubiquitination that target the protein for proteasomal degradation; however, the precise biological functions of these modifications remained unknown. By exogenous expression of mutant DDB2 proteins in normal human fibroblasts, here we show that the N-terminal tail of DDB2 is involved in regulation of cellular responses to UV. By striking contrast with behaviors of exogenous DDB2, the endogenous DDB2 protein was stabilized even after UV irradiation as a function of the XPC expression level. Furthermore, XPC competitively suppressed ubiquitination of DDB2 in vitro, and this effect was significantly promoted by centrin-2, which augments the DNA damage-recognition activity of XPC. Based on these findings, we propose that in cells exposed to UV, DDB2 is protected by XPC from ubiquitination and degradation in a stochastic manner; thus XPC allows DDB2 to initiate multiple rounds of repair events, thereby contributing to the persistence of cellular DNA repair capacity.
NTF2-like domain of Tap plays a critical role in cargo mRNA recognition and export
Authors: Katahira Jun , Dimitrova Lyudmila , Imai Yumiko , Hurt Ed 
Published: January 2015

Abstract
Metazoan Tap-p15 (also called Nxf1-Nxt1) and yeast Mex67-Mtr2 heterodimers are the general mRNA export receptors. The RNA binding activity of Tap-p15, which is essential for mRNA nuclear export, has been attributed to the amino-terminal RNA binding module of Tap consists of RNA recognition motif (RRM) and leucine-rich repeat. In this study, we identified a novel RNA interaction surface in the NTF2-like (NTF2L) domain of Tap, which is analogous to the rRNA binding platform of Mex67-Mtr2. Tap-p15 uses the three domains to tightly bind the retroviral constitutive transport element. The RNA binding through the NTF2L domain is functionally relevant as introduction of mutations in this region reduced CTE-containing mRNA export activity. In contrast, only when the RRM and NTF2L domains were mutated simultaneously, bulk poly (A)+ RNA export and in vivo poly (A)+ RNA binding activities of Tap-p15 were significantly attenuated. Moreover, an engineered human cell line harboring the NTF2L domain mutation in the NXF1 gene showed a synthetic growth phenotype and severe mRNA export defect under Aly/REF and Thoc5 depleted condition. These data suggest that Tap-p15 recognizes bulk mRNAs through combinatorial use of the distinct RNA binding domains.
Engineered ribosomal RNA operon copy-number variants of E. coli reveal the evolutionary trade-offs shaping rRNA operon number
Authors: Gyorfy Zsuzsanna , Draskovits Gabor , Vernyik Viktor , Blattner Frederick F. , Gaal Tamas , Posfai Gyorgy 
Published: January 2015

Abstract
Ribosomal RNA (rrn) operons, characteristically present in several copies in bacterial genomes (7 in E. coli), play a central role in cellular physiology. We investigated the factors determining the optimal number of rrn operons in E. coli by constructing isogenic variants with 5–10 operons. We found that the total RNA and protein content, as well as the size of the cells reflected the number of rrn operons. While growth parameters showed only minor differences, competition experiments revealed a clear pattern: 7–8 copies were optimal under conditions of fluctuating, occasionally rich nutrient influx and lower numbers were favored in stable, nutrient-limited environments. We found that the advantages of quick adjustment to nutrient availability, rapid growth and economic regulation of ribosome number all contribute to the selection of the optimal rrn operon number. Our results suggest that the wt rrn operon number of E. coli reflects the natural, ‘feast and famine’ life-style of the bacterium, however, different copy numbers might be beneficial under different environmental conditions. Understanding the impact of the copy number of rrn operons on the fitness of the cell is an important step towards the creation of functional and robust genomes, the ultimate goal of synthetic biology.