bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two-step process for preparation of oligosaccharide propionates and acrylates using lipase and Cyclodextrin Glycosyl Transferase (CGTase)
Authors: Ayres Bianca T , Valença Gustavo P , Franco Telma T , Adlercreutz Patrick 
Published: March 2014

Abstract

Background
Oligosaccharide esters are attractive candidates for applications as surfactants, hydrogels and other materials, but direct enzymatic acylation is difficult with carbohydrates longer than disaccharides.

Results
A combination of one lipase-catalyzed step and one transglycosylation step catalyzed by a cyclodextrin glycosyl transferase (CGTase) was used to synthesize oligosaccharide esters. The conversion of glucose and maltose with vinyl propionate catalyzed by Candida antarctica lipase B (Novozym 435) in dioxane proceeded to full conversion to mixtures of mono and diesters. When ethyl acrylate was used as acyl donor, mono and diesters were formed, but full conversion was not reached. The CGTase catalyzed reactions between the glucose and maltose esters and α-cyclodextrin were carried out in water. In the initial phase, addition of the glucose residues of the cyclodextrin to the ester substrate occurred (coupling reaction), followed by disproportionation reactions yielding a range of oligosaccharide esters with varying chain length. The monoesters were efficient acceptors in the CGTase-catalyzed reactions, while the diesters were not converted to a significant extent. As a consequence, the glucose propionate which contained large amounts of diesters was converted to 40% conversion while the maltose propionate which contained mainly monoesters was converted to 86% conversion.

Conclusions
A two-step enzymatic process for preparation of oligosaccharide esters has been developed. Oligosaccharide propionates were produced in high yield with a total reaction time of 5 h. The double bond of the acrylate moiety reduced the reaction rate of the lipase catalyzed transesterification, but in both cases, the CGTase efficiently converted the monoesters to oligosaccharide esters.
[18F]DPA-714 as a biomarker for positron emission tomography imaging of rheumatoid arthritis in an animal model
Authors: Pottier Géraldine , Bernards Nicholas , Dollé Frédéric , Boisgard Raphael 
Published: March 2014

Abstract
Introduction
Rheumatoid arthritis (RA) is a chronic disease, affecting 0.5 to 1% of adults in industrialized countries, in which systemic inflammation and synovitis drive joint destruction. [18F]DPA-714 is a specific tracer of the 18 kDa translocator protein (TSPO), which is overexpressed on activated macrophages, and proposed as a biomarker of neuroinflammation. Today, diagnosis of patients with early inflammatory arthritis is limited by poor sensitivity and specificity. The present study aims to investigate the potential of [18F]DPA-714 to monitor in vivo inflammatory processes at a preclinical stage via positron emission tomography (PET).

Methods
RA was induced in Dark Agouti rats by subcutaneous injection of inactivated Mycobacterium tuberculosis. Development of arthritis clinical signs was investigated daily and the severity of the disease evaluated. Animals were imaged at the peak of inflammation using [18F]DPA-714 and a small-animal PET-CT tomograph.

Results
The first clinical signs appeared at 10 days post-injection, with a peak of inflammation at 20 days. At this time, PET-analyses showed a clear uptake of [18F]DPA-714 in swollen ankles, with mean values of 0.52 ± 0.18% injected dose (ID/cc) for treated (n = 11) and 0.19 ± 0.09 for non-treated (n = 6) rats. A good correlation between [18F]DPA-714’s uptake and swelling was also found. Immunohistochemistry showed an enhanced TSPO expression in hind paws, mainly co-localized with the macrophages specific antigen CD68 expressing cells.

Conclusion
These preliminary results demonstrate that the TSPO 18kDa specific radioligand [18F]DPA-714 is adapted for the study and follow-up of inflammation linked to RA in our experimental model, suggesting also a strong potential for clinical imaging of peripheral inflammation.
Vacuolar-ATPase isoform a2 regulates macrophages and cytokine profile necessary for normal spermatogenesis in testis
Authors: Jaiswal Mukesh K , Katara Gajendra K , Mallers Timothy , Chaouat Gerard , Gilman-Sachs Alice , Beaman Kenneth D 
Published: March 2014

Abstracta2V is required for maturation of sperm. The decreased expression of a2V at the feto-maternal interphase causes poor pregnancy outcome. The present study examined the role of a2V in spermatogenesis and inflammatory network in the testis. A single dose of anti-a2V mouse IgG or mouse IgG isotype (3 μg/animal) was injected i.p. into male mice on alternate days for 10 days. Anti-a2V-treated males exhibit severe deficiencies of spermatogenesis, which is indicated by the presence of less numbers of postmeiotic cells. Sperm counts and sperm motility were reduced significantly in anti-a2V-treated males. The release of the cleaved a2NTD was significantly lower in anti-a2V-treated testes. The TMs were identified as M2-like macrophages, and this population and the expression of various cytokines/chemokines (Tgf-β, Il-6, Nos2, Tnf, Lif, Mcp1, Ccl5) were decreased significantly in anti-a2V-treated testis compared with control testis. Moreover, the cleaved a2NTD acts as a key mediator of TMs and significantly up-regulates the secretion of testicular cytokines/chemokines, which are associated with normal spermatogenesis. When these anti-a2V-treated males were used for mating with normal females, the number of implantation sites was decreased significantly in the females mated with anti-a2V-treated males than the females mated with control males. These observations suggest that a2V plays a crucial role in spermatogenesis by regulating testicular immune responses, and its inhibition in males leads to poor pregnancy outcome in females.
Survey of stereotactic ablative body radiotherapy in the UK by the QA group on behalf of the UK SABR Consortium
Authors: Distefano G , Baker A , Scott A J D , Webster G J ,   on behalf of the UK SABR Consortium Quality Assurance Group
Published: March 2014

Objective:
To ascertain the progress being made towards the implementation of stereotactic ablative body radiotherapy (SABR) treatment in the UK, to obtain details of current practice in centres with an active treatment programme and to assess the projected future provision.

Methods:
In August 2012, an online questionnaire was sent to all 65 UK radiotherapy institutions. The included questions covered the current number of patients being treated and the intended number of patients for each clinical site; immobilization and motion management methods; CT scanning protocols; target and organ-at-risk delineation; treatment planning; image-guidance and treatment protocols; and quality assurance methods.

Results:
48/65 (74%) institutions responded by the end of November 2012, with 15 indicating an active SABR programme. A further four centres indicated that a SABR protocol had been established but was not yet in clinical use. 14 of the 29 remaining responses stated an intention to develop a SABR programme in the next 2 years.

Conclusion:
The survey responses confirm that SABR provision in the UK is increasing and that this should be expected to continue in the next 2 years. A projection of the future uptake would suggest that by the end of 2014, UK SABR provision will be broadly in line with international practice.
Balancing Acquisition and Retention Spending for Firms with Limited Capacity
Authors: Ovchinnikov Anton , Boulu-Reshef Béatrice , Pfeifer Phillip E. 
Published: March 2014

This paper discusses the interaction between revenue management and customer relationship management for a firm that operates in a customer retention situation but faces limited capacity. We present a dynamic programming model for how the firm balances investments in customer acquisition and retention, as well as retention across multiple customer types. We characterize the optimal policy and discuss how the policy changes depending on capacity limitations. We then contrast the modeling results with those of a behavioral experiment in which subjects acted as managers making acquisition and retention decisions. In the modeling part of the paper, we introduce a concept of the value of an incremental customer (VIC), and show that when capacity is unlimited, VIC equals customer lifetime value (CLV), but when capacity is limited, VIC is much smaller and changes dynamically depending on the number of customers and their mix. As a result, the optimal spending is constant and depends on CLV for the firms with unlimited capacity, but changes dynamically and is generally unrelated to CLV when capacity is limited. In the experimental part, we introduce a concept of conditional optimality for the analysis of state-dependent decisions. Applying this concept to our data, we document a number of decision biases, specifically the subjects' tendency to overspend on retaining high-value customers and underspend on lower-value customers retention and acquisition. We show that providing CLV information exacerbates these biases and leads to a loss of net revenue when capacity is limited, but providing information about the marginal costs of acquisition and retention eliminated these biases and increases net revenue. Data, as supplemental material, are available at http://dx.doi.org/10.1287/mnsc.2013.1842 . This paper was accepted by Yossi Aviv, operations management.
Effect of compression wood on dimensional stability of medium density fiberboard
Authors: Ayrilmis Nadir 
Published: March 2014

This study evaluated the effect of compression wood on dimensional stability of medium density fiberboards (MDF) manufactured from fiber furnishes of pine (Pinus nigra Arnold var. pallasiana) containing compression wood. Two panel types were manufactured from two different compression wood (CW) portion / normal wood (NW) portions in the furnish, 75/25 and 10/90, respectively. Linear and thickness variations of the panels exposed to various relative humidites at 20Â Â°C, linear expansion/contraction and thickness swelling/shrinkage, were measured according to the procedures defined by DIN EN 318 (2005) standard test method. Panels made from fiber furnish containing 75% the CW had higher linear expansion and linear contraction values with an average value of 0.286% and 0.247% than those of panels made from fiber furnish containing 10% the CW with an average value of 0.184% and 0.152%, respectively. As for thickness swelling and thickness shrinkage properties, panels made from fiber furnish containing 75% the CW had the thickness swelling and thickness shrinkage values with an average of 5.042% and 4.402% while panels made from fiber furnish containing 10% the CW had the values with 3.621% and 2.861%, respectively. Consequently, based on the findings obtained from this study, expansion and swelling properties of the MDF panels were negatively affected by compression wood increase.
„Glaub nicht immer, daß Du Deine Worte von Tatsachen abliest.“
Authors: Arnswald Ulrich 
Published: March 2014

Zusammenfassung
Erinnerung ist für Wittgenstein ein komplexer Vorgang, der mit einer Vielzahl von Schwierigkeiten verbunden ist. Dies gilt besonders für seine Überlegungen zur Erinnerung einer Absicht – ein in der Wittgenstein-Literatur bis dato vernachlässigter Aspekt, obwohl er in diversen Manuskripten des Nachlasses hervorsticht. Nach Wittgenstein erlaubt uns der Erinnerungsvorgang nicht, uns eine vermeintliche Absicht „vor Augen zu führen“, dennoch verwenden wir den sprachlichen Ausdruck, um mittels einer Erinnerungsbeschreibung eine vergangene Absicht zu äußern. Nur was bringen wir damit aber zum Ausdruck? – Da die Worte sich weder auf Tatsachen beziehen noch mit diesen korrespondieren, kommt es zur paradoxen Situation, dass jemand sich des Inhalts einer Absicht zu erinnern meint, nicht aber der einstigen Worte. Dies wirft im Umkehrschluss die Frage auf, was unter diesen Umständen die Artikulation der Erinnerung einer Absicht überhaupt aussagen soll und kann, und weiterhin, was der Unterschied zu einer gegenwärtigen Absichtserklärung ist.
Physicochemical Studies of Aqueous Amphiphilic Drug + Hydrotrope Solutions
Authors: Kabir-ud-Din  , Al-dahbali Gamal A. , Naqvi Andleeb Z. , Akram Mohd. 
Published: March 2014

Abstract
The micellization and interfacial behaviours of two amphiphilic drugs
(adephinine and clomipramine hydrochlorides) in the presence of
hydrotropes (sodium salicylate and sodium benzoate) have been
investigated by surface tension measurements. Micellar and interfacial
compositions at different stoichiometeric mole fractions of the
hydrotropes have been evaluated using Rubingh's and Rosen's
models. Synergism was found in mixed micelles as well as in mixed
monolayers. Thermodynamic parameters (ΔG
mic

o
,
ΔG
ex, G
min , ΔG
ads

o
)
indicate that mixed systems are more stable than the individual
systems with CLP-hydrotrope > ADP-hydrotrope systems.
The SKPO-1 Peroxidase Functions in the Hypodermis to ProtectCaenorhabditis elegansFrom Bacterial Infection
Authors: Tiller George R , Garsin Danielle A 
Published: March 2014

AbstractIn recent years, the synergistic relationship between NADPH oxidase (NOX)/dual oxidase (DUOX) enzymes and peroxidases has received increased attention. Peroxidases utilize NOX/DUOX-generated H2O2 for a myriad of functions including, but not limited to, thyroid hormone biosynthesis, cross-linking extracellular matrices (ECM), and immune defense. We postulated that one or more peroxidases produced by Caenorhabditis elegans would act in host defense, possibly in conjunction with BLI-3, the only NOX/DUOX enzyme encoded by the genome that is expressed. Animals exposed to RNA interference (RNAi) of the putative peroxidase genes were screened for susceptibility to the human pathogen Enterococcus faecalis. One of three genes identified, skpo-1 (ShkT-containing peroxidase), was studied in depth. Animals mutant for this gene were significantly more susceptible to E. faecalis, but not Pseudomonas aeruginosa. A slight decrease in longevity was also observed. The skpo-1 mutant animals had a dumpy phenotype of incomplete penetrance; half the animals displayed a dumpy phenotype ranging from slight to severe, and half were morphologically wild type. The SKPO-1 protein contains the critical catalytic residues necessary for peroxidase activity, and in a whole animal assay, more H2O2 was detected from the mutant compared to the wild type, consistent with the loss of an H2O2 sink. By using tissue-specific skpo-1 RNAi and immunohistochemical localization with an anti-SKPO-1 antibody, it was determined that the peroxidase is functionally and physically present in the hypodermis. In conclusion, these results characterize a peroxidase that functions protectively in the hypodermis during exposure to E. faecalis.
Annealing pressure dependence of Cu2ZnSnSe4 composition and properties
Authors: Yu Zhou , Liu Lian , Yan Yong , Li Shasha , Jin Yaxin , Ou Yufeng , Yan Chuanpeng , Zhang Yong , Zhao Yong 
Published: March 2014

ABSTRACTCu2ZnSnSe4 (CZTSe) precursor films were deposited by one-step RF sputtering process at room temperature under various sputtering power, and then films were annealed at different pressure of 10-3 Pa and 100 Pa. Films annealed at high vacuum of 10-3 Pa exhibit significant loss of Sn element and they construct with two phases of Cu1.8Se and ZnSe. Higher annealing pressure at 100 Pa can drastically reduce the loss of Sn element and result in single kesterite CZTSe phase of the annealed films. Loss of Se element is found in all the annealed films and the values of [Se]/[Metal] and [Sn]/[Zn] are related with sputtering power. High vacuum annealed films show cracks and porous structure on the surface, meanwhile, films annealed at 100 Pa show compact, densely packed homogeneous morphology.
Structure, dynamics and primitive path network of polymer nanocomposites containing spherical nanoparticles
Authors: Karatrantos Argyrios , Clarke Nigel , Composto Russel J. , Winey Karen I. 
Published: March 2014

ABSTRACTWe investigate the effect of nanoparticles on polymer structure, polymer dimensions and topological constraints (entanglements) in polymer melts for nanoparticle loading above percolation threshold as high as 40.9% using stochastic molecular dynamics (MD) simulations. We show unambiguously that short polymer chains are not disturbed by the presence of repulsive nanoparticles. In contrast entangled polymer chains can be perturbed by the presence of attractive nanoparticles when the polymer radius of gyration is larger than the nanoparticle radius. They can expand under the presence of attractive nanoparticles even at low nanoparticle loadings of very small nanoparticle size. We observe an increase in the number of entanglements (decrease of Ne with 40.9% volume fraction of nanoparticles dispersed in the polymer matrix) in the nanocomposites as evidenced by larger contour lengths of the primitive paths. Attraction between polymers and nanoparticles affects the entanglements in the nanocomposites and alters the primitive path.
Relation Between Higher Physical Activity and Public Transit Use
Authors: Saelens Brian E. , Vernez Moudon Anne , Kang Bumjoon , Hurvitz Philip M. , Zhou Chuan 
Published: March 2014

Objectives. We isolated physical activity attributable to transit use to examine issues of substitution between types of physical activity and potential confounding of transit-related walking with other walking. Methods. Physical activity and transit use data were collected in 2008 to 2009 from 693 Travel Assessment and Community study participants from King County, Washington, equipped with an accelerometer, a portable Global Positioning System, and a 7-day travel log. Physical activity was classified into transit- and non–transit-related walking and nonwalking time. Analyses compared physical activity by type between transit users and nonusers, between less and more frequent transit users, and between transit and nontransit days for transit users. Results. Transit users had more daily overall physical activity and more total walking than did nontransit users but did not differ on either non–transit-related walking or nonwalking physical activity. Most frequent transit users had more walking time than least frequent transit users. Higher physical activity levels for transit users were observed only on transit days, with 14.6 minutes (12.4 minutes when adjusted for demographics) of daily physical activity directly linked with transit use. Conclusions. Because transit use was directly related to higher physical activity, future research should examine whether substantive increases in transit access and use lead to more physical activity and related health improvements.
Socioeconomic Status, Food Security, and Dental Caries in US Children: Mediation Analyses of Data From the National Health and Nutrition Examination Survey, 2007–2008
Authors: Chi Donald L. , Masterson Erin E. , Carle Adam C. , Mancl Lloyd A. , Coldwell Susan E. 
Published: March 2014

Objectives. We examined associations of household socioeconomic status (SES) and food security with children’s oral health outcomes. Methods. We analyzed 2007 and 2008 US National Health and Nutrition Examination Survey data for children aged 5 to 17 years (n = 2206) to examine the relationship between food security and untreated dental caries and to assess whether food security mediates the SES–caries relationship. Results. About 20.1% of children had untreated caries. Most households had full food security (62%); 13% had marginal, 17% had low, and 8% had very low food security. Higher SES was associated with significantly lower caries prevalence (prevalence ratio [PR] = 0.77; 95% confidence interval = 0.63, 0.94; P = .01). Children from households with low or very low food security had significantly higher caries prevalence (PR = 2.00 and PR = 1.70, respectively) than did children living in fully food-secure households. Caries prevalence did not differ among children from fully and marginally food-secure households (P = .17). Food insecurity did not appear to mediate the SES–caries relationship. Conclusions. Interventions and policies to ensure food security may help address the US pediatric caries epidemic.
“I Did It for Us and I Would Do It Again”: Perspectives of Rural Latinos on Providing Biospecimens for Research
Authors: Hohl Sarah D. , Gonzalez Claire , Carosso Elizabeth , Ibarra Genoveva , Thompson Beti 
Published: March 2014

Objectives. We elicited perspectives of rural Latino farmworkers and non-farmworkers about their participation in a community-based participatory pesticides exposure study in which they provided multiple biospecimens. Methods. Between March and April 2012, we conducted semistructured, one-on-one interviews with 39 rural Latino farmworkers and non-farmworkers in Washington State (n = 39). Nineteen open-ended interview questions aimed to elicit participants’ attitudes toward, expectations and experiences of biospecimen collection for research, and willingness to participate in future biomedical research studies. We reviewed and coded transcriptions using qualitative principles of grounded theory in which concepts were identified and themes derived from interview data. Results. We grouped themes into 3 major categories: (1) motivation to participate, (2) challenges of participation, and (3) perceived rewards of participation. Many participants were motivated by the perceived importance of the study topic and a desire to acquire and contribute to new knowledge. Respondents said that the benefits of participation outweighed the challenges, and many expressed satisfaction to be able to contribute to research that would benefit future generations. Conclusions. Our findings supported the use of community-based participatory research to engage minorities as participants and invested parties in such studies.
Long-Term Consequences of Adolescent Gang Membership for Adult Functioning
Authors: Gilman Amanda B. , Hill Karl G. , Hawkins J. David 
Published: March 2014

Objectives. We examined the possible public health consequences of adolescent gang membership for adult functioning.Methods. Data were drawn from the Seattle Social Development Project, a longitudinal study focusing on the development of positive and problem outcomes. Using propensity score matching and logistic regression analyses, we assessed the effects of adolescent gang membership on illegal behavior, educational and occupational attainment, and physical and mental health at the ages of 27, 30, and 33 years.Results. In comparison with their nongang peers, who had been matched on 23 confounding risk variables known to be related to selection into gang membership, those who had joined a gang in adolescence had poorer outcomes in multiple areas of adult functioning, including higher rates of self-reported crime, receipt of illegal income, incarceration, drug abuse or dependence, poor general health, and welfare receipt and lower rates of high school graduation.Conclusions. The finding that adolescent gang membership has significant consequences in adulthood beyond criminal behavior indicates the public health importance of the development of effective gang prevention programs.