bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two-Dimensional Fluorescence In-Gel Electrophoresis of Coronary Restenosis Tissues in Minipigs
Authors: Lu Lin , Wang Ya Nan , Sun Wei Hua , Liu Zhu Hui , Zhang Qi , Pu Li Jin , Yang Ke , Wang Ling Jie , Zhu Zhen Bin , Meng Hua , Yang Ping , Du Run , Chen Qiu Jing , Wang Li Shun , Yu Hong , Shen Wei Feng 
Published: February 2013

Objective—
We aimed to uncover the protein changes of coronary artery in-stent restenosis (ISR) tissue in minipigs with and without streptozotocin-induced diabetes mellitus by quantitative 2-dimensional fluorescence in-gel electrophoresis (2D-DIGE), and to investigate the influences of crucial proteins identified, particularly adipocyte fatty acid binding protein (AFABP), in human arterial smooth muscle cells.

Methods and Results—
Sirolimus-eluting stents were implanted in the coronary arteries of 15 diabetic and 26 nondiabetic minipigs, and angiography was repeated after 6 months. The intima tissue of significant ISR and non-ISR segments in both diabetic and nondiabetic minipigs was analyzed by 2D-DIGE and MALDI-TOF/TOF mass spectrometry. AFABP level was significantly increased in ISR tissue than in non-ISR tissue in both diabetic and nondiabetic minipigs, with level being higher in diabetic ISR than in nondiabetic ISR tissue. In human arterial smooth muscle cells, overexpression of AFABP significantly altered phenotype and promoted growth and migration, with effects more prominent in high-glucose than in low-glucose medium, whereas AFABP knockdown inhibited these effects. AFABP overexpression increased reactive oxygen species production by upregulating the expression of NADPH oxidase subunits Nox1, Nox4, and P22 through multiple pathways, with elevation of downstream gene cyclin D1, matrix metalloproteinase-2, and monocyte chemoattractant protein-1. However, AFABP-induced effects were inhibited by diphenyleneiodonium, pathway inhibitors, and small interfering RNA. In addition, the supernatant from AFABP-expressing human arterial smooth muscle cells and recombinant AFABP also promoted cellular growth and migration.

Conclusion—
This study has demonstrated that AFABP is significantly increased in coronary artery ISR segments of both diabetic and nondiabetic minipigs. Increased AFABP expression and secretory AFABP of human arterial smooth muscle cells promote growth and migration via reactive oxygen species-mediated activation.
New White Matter Brain Injury After Infant Heart Surgery Is Associated With Diagnostic Group and the Use of Circulatory Arrest
Authors: Beca John , Gunn Julia K. , Coleman Lee , Hope Ayton , Reed Peter W. , Hunt Rodney W. , Finucane Kirsten , Brizard Christian , Dance Brieana , Shekerdemian Lara S. 
Published: February 2013

Background—
Abnormalities on magnetic resonance imaging scans are common both before and after surgery for congenital heart disease in early infancy. The aim of this study was to prospectively investigate the nature, timing, and consequences of brain injury on magnetic resonance imaging in a cohort of young infants undergoing surgery for congenital heart disease both with and without cardiopulmonary bypass.

Methods and Results—

A total of 153 infants undergoing surgery for congenital heart disease at <8 weeks of age underwent serial magnetic resonance imaging scans before and after surgery and at 3 months of age, as well as neurodevelopmental assessment at 2 years of age. White matter injury (WMI) was the commonest type of injury both before and after surgery. It occurred in 20% of infants before surgery and was associated with a less mature brain. New WMI after surgery was present in 44% of infants and at similar rates after surgery with or without cardiopulmonary bypass. The most important association was diagnostic group (
P
<0.001). In infants having arch reconstruction, the use and duration of circulatory arrest were significantly associated with new WMI. New WMI was also associated with the duration of cardiopulmonary bypass, postoperative lactate level, brain maturity, and WMI before surgery. Brain immaturity but not brain injury was associated with impaired neurodevelopment at 2 years of age.

Conclusions—
New WMI is common after surgery for congenital heart disease and occurs at the same rate in infants undergoing surgery with and without cardiopulmonary bypass. New WMI is associated with diagnostic group and, in infants undergoing arch surgery, the use of circulatory arrest.
Guidelines for the Early Management of Patients With Acute Ischemic Stroke
Authors: Jauch Edward C. , Saver Jeffrey L. , Adams Harold P. , Bruno Askiel , Connors J.J. (Buddy) , Demaerschalk Bart M. , Khatri Pooja , McMullan Paul W. , Qureshi Adnan I. , Rosenfield Kenneth , Scott Phillip A. , Summers Debbie R. , Wang David Z. , Wintermark Max , Yonas Howard 
Published: February 2013

Background and Purpose—
The authors present an overview of the current evidence and management recommendations for evaluation and treatment of adults with acute ischemic stroke. The intended audiences are prehospital care providers, physicians, allied health professionals, and hospital administrators responsible for the care of acute ischemic stroke patients within the first 48 hours from stroke onset. These guidelines supersede the prior 2007 guidelines and 2009 updates.

Methods—
Members of the writing committee were appointed by the American Stroke Association Stroke Council’s Scientific Statement Oversight Committee, representing various areas of medical expertise. Strict adherence to the American Heart Association conflict of interest policy was maintained throughout the consensus process. Panel members were assigned topics relevant to their areas of expertise, reviewed the stroke literature with emphasis on publications since the prior guidelines, and drafted recommendations in accordance with the American Heart Association Stroke Council’s Level of Evidence grading algorithm.

Results—
The goal of these guidelines is to limit the morbidity and mortality associated with stroke. The guidelines support the overarching concept of stroke systems of care and detail aspects of stroke care from patient recognition; emergency medical services activation, transport, and triage; through the initial hours in the emergency department and stroke unit. The guideline discusses early stroke evaluation and general medical care, as well as ischemic stroke, specific interventions such as reperfusion strategies, and general physiological optimization for cerebral resuscitation.

Conclusions—
Because many of the recommendations are based on limited data, additional research on treatment of acute ischemic stroke remains urgently needed.
The Modified Graeb Score
Authors: Morgan Timothy C. , Dawson Jesse , Spengler Danielle , Lees Kennedy R. , Aldrich Chanel , Mishra Nishant K. , Lane Karen , Quinn Terence J. , Diener-West Marie , Weir Christopher J. , Higgins Peter , Rafferty Mark , Kinsley Katie , Ziai Wendy , Awad Issam , Walters Matthew R. , Hanley Daniel 
Published: February 2013

Background and Purpose—
Simple and rapid measures of intraventricular hemorrhage (IVH) volume are lacking. We developed and validated a modification of the original Graeb scale to facilitate rapid assessment of IVH over time.

Methods—
We explored the relationship between the modified Graeb scale (mGS), original Graeb scale, measured IVH volume, and outcome using data from the Clot Lysis: Evaluating Accelerated Resolution of Hemorrhage with rtPA B (CLEAR B) study. We also explored its reliability. We then evaluated the relationship between mGS and outcome in a large sample of participants with IVH using data contained within the Virtual International Stroke Trials Archive (VISTA). We defined outcome using the modified Rankin scale (>3 signifying poor outcome).

Results—

The CLEAR B study included 360 scans from 36 subjects. The mGS score and IVH volume were highly correlated (R = 0.80,
P
<0.0001,
R
2
0.65). Baseline mGS was predictive of poor outcome (area under receiving operating characteristic curve 0.74, 95% confidence interval, 0.57–0.91), whereas the original Graeb scale was not. The VISTA study included 399 participants. Each unit increase in the mGS led to a 12% increase in the odds of a poor outcome (odds ratio, 1.12; 95% confidence interval, 1.05–1.19). Measures of reliability (intra- and inter- reader) were good in both studies.

Conclusions—
The mGS, a semiquantitative scale for IVH volume measurement, is a reliable measure with prognostic validity suitable for rapid use in clinical practice and in research.
Is Intracerebral Hemorrhage a Time-Dependent Phenomenon After Successful Combined Intravenous and Intra-Arterial Therapy?
Authors: Mosimann Pascal J. , Sirimarco Gaia , Meseguer Elena , Serfaty Jean-Michel , Laissy Jean-Pierre , Labreuche Julien , Lapergue Bertrand , Gonzalez-Valcarcel Jaime , Lavallée Philippa C. , Cabrejo Lucie , Guidoux Celine , Klein Isabelle F. , Olivot Jean-Marc , Schouman-Claeys Elisabeth , Amarenco Pierre , Mazighi Mikael 
Published: February 2013

Background and Purpose—
Onset-to-reperfusion time (ORT) has recently emerged as an essential prognostic factor in acute ischemic stroke therapy. Although favorable outcome is associated with reduced ORT, it remains unclear whether intracranial bleeding depends on ORT. We therefore sought to determine whether ORT influenced the risk and volume of intracerebral hemorrhage (ICH) after combined intravenous and intra-arterial therapy.

Methods—
Based on our prospective registry, we included 157 consecutive acute ischemic stroke patients successfully recanalized with combined intravenous and intra-arterial therapy between April 2007 and October 2011. Primary outcome was any ICH within 24 hours posttreatment. Secondary outcomes included occurrence of symptomatic ICH (sICH) and ICH volume measured with the ABC/2.

Results—

Any ICH occurred in 26% of the study sample (n=33). sICH occurred in 5.5% (n=7). Median ICH volume was 0.8 mL. ORT was increased in patients with ICH (median=260 minutes; interquartile range=230–306) compared with patients without ICH (median=226 minutes; interquartile range=200–281;
P
=0.008). In the setting of sICH, ORT reached a median of 300 minutes (interquartile range=276–401;
P
=0.004). The difference remained significant after adjustment for potential confounding factors (adjusted
P
=0.045 for ICH; adjusted
P
=0.002 for sICH). There was no correlation between ICH volume and ORT (
r
=0.16;
P
=0.33).

Conclusions—
ORT influences the rate but not the volume of ICH and appears to be a critical predictor of symptomatic hemorrhage after successful combined intravenous and intra-arterial therapy. To minimize the risk of bleeding, revascularization should be achieved within 4.5 hours of stroke onset.
Sensitivity of Warm-Frontal Processes to Cloud-Nucleating Aerosol Concentrations
Authors: Igel Adele L. , van den Heever Susan C. , Naud Catherine M. , Saleeby Stephen M. , Posselt Derek J. 
Published: February 2013

Abstract
An extratropical cyclone that crossed the United States on 9–11 April 2009 was successfully simulated at high resolution (3-km horizontal grid spacing) using the Colorado State University Regional Atmospheric Modeling System. The sensitivity of the associated warm front to increasing pollution levels was then explored by conducting the same experiment with three different background profiles of cloud-nucleating aerosol concentration. To the authors’ knowledge, no study has examined the indirect effects of aerosols on warm fronts. The budgets of ice, cloud water, and rain in the simulation with the lowest aerosol concentrations were examined. The ice mass was found to be produced in equal amounts through vapor deposition and riming, and the melting of ice produced approximately 75% of the total rain. Conversion of cloud water to rain accounted for the other 25%. When cloud-nucleating aerosol concentrations were increased, significant changes were seen in the budget terms, but total precipitation remained relatively constant. Vapor deposition onto ice increased, but riming of cloud water decreased such that there was only a small change in the total ice production and hence there was no significant change in melting. These responses can be understood in terms of a buffering effect in which smaller cloud droplets in the mixed-phase region lead to both an enhanced vapor deposition and decreased riming efficiency with increasing aerosol concentrations. Overall, while large changes were seen in the microphysical structure of the frontal cloud, cloud-nucleating aerosols had little impact on the precipitation production of the warm front.
Delineating the Eddy–Zonal Flow Interaction in the Atmospheric Circulation Response to Climate Forcing: Uniform SST Warming in an Idealized Aquaplanet Model
Authors: Chen Gang , Lu Jian , Sun Lantao 
Published: February 2013

Abstract
The mechanisms of the atmospheric response to climate forcing are analyzed using an example of uniform SST warming in an idealized aquaplanet model. A 200-member ensemble of experiments is conducted with an instantaneous uniform SST warming. The zonal mean circulation changes display a rapid poleward shift in the midlatitude eddy-driven westerlies and the edge of the Hadley cell circulation and a slow equatorward contraction of the circulation in the deep tropics. The shift of the poleward edge of the Hadley cell is predominantly controlled by the eddy momentum flux. It also shifts the eddy-driven westerlies against the surface friction, at a rate much faster than the expectation from the natural variability of the eddy-driven jet (i.e., the e-folding time scale of the annular mode), with much less feedback between the eddies and zonal flow.
The transient eddy–zonal flow interactions are delineated using a newly developed finite-amplitude wave activity diagnostic of Nakamura. Applying it to the transient ensemble response to uniform SST warming reveals that the eddy-driven westerlies are shifted poleward by permitting more upward wave propagation in the middle and upper troposphere rather than reducing the lower-tropospheric baroclinicity. The increased upward wave propagation is attributed to a reduction in eddy dissipation of wave activity as a result of a weaker meridional potential vorticity (PV) gradient. The reduction allows more waves to propagate away from the latitudes of baroclinic generation, which, in turn, leads to more poleward momentum flux and a poleward shift of eddy-driven winds and Hadley cell edge.
Evaluation of Precipitation Simulated by Seven SCMs against the ARM Observations at the SGP Site*
Authors: Song Hua , Lin Wuyin , Lin Yanluan , Wolf Audrey B. , Neggers Roel , Donner Leo J. , Del Genio Anthony D. , Liu Yangang 
Published: February 2013

Abstract
This study evaluates the performances of seven single-column models (SCMs) by comparing simulated surface precipitation with observations at the Atmospheric Radiation Measurement Program Southern Great Plains (SGP) site from January 1999 to December 2001. Results show that although most SCMs can reproduce the observed precipitation reasonably well, there are significant and interesting differences in their details. In the cold season, the model–observation differences in the frequency and mean intensity of rain events tend to compensate each other for most SCMs. In the warm season, most SCMs produce more rain events in daytime than in nighttime, whereas the observations have more rain events in nighttime. The mean intensities of rain events in these SCMs are much stronger in daytime, but weaker in nighttime, than the observations. The higher frequency of rain events during warm-season daytime in most SCMs is related to the fact that most SCMs produce a spurious precipitation peak around the regime of weak vertical motions but rich in moisture content. The models also show distinct biases between nighttime and daytime in simulating significant rain events. In nighttime, all the SCMs have a lower frequency of moderate-to-strong rain events than the observations for both seasons. In daytime, most SCMs have a higher frequency of moderate-to-strong rain events than the observations, especially in the warm season. Further analysis reveals distinct meteorological backgrounds for large underestimation and overestimation events. The former occur in the strong ascending regimes with negative low-level horizontal heat and moisture advection, whereas the latter occur in the weak or moderate ascending regimes with positive low-level horizontal heat and moisture advection.
Spatially Inhomogeneous Trends of Tropical Cyclone Intensity over the Western North Pacific for 1977–2010
Authors: Park Doo-Sun R. , Ho Chang-Hoi , Kim Joo-Hong , Kim Hyeong-Seog 
Published: February 2013

Abstract
The spatial distribution of trends in tropical cyclone (TC) intensity over the western North Pacific Ocean (WNP) during the period 1977–2010 was examined using five TC datasets. The spatial distribution of the TC intensity was expressed by seasonally averaged maximum wind speeds in 5° × 5° horizontal grids. The trends showed a spatial inhomogeneity, with a weakening in the tropical Philippine Sea (TP) and a strengthening in southern Japan and its southeastern ocean (SJ). This distribution could be described by TC intensification rate and genesis frequency, with the aid of the climatological direction of TC movement. The increasing intensification rate around the center of the WNP could mostly account for the increasing intensity over the SJ region, while the influence of both intensification rate and local genesis frequency mattered in the TP region because of the effect of the newly generated and less-developed weak TCs on the TC intensity. Thermodynamic variables (e.g., sea surface temperature, potential intensity, and 26°C isotherm depth) showed almost homogeneous changes in space, possibly favoring intensification rate and genesis frequency over the entire WNP. However, the decreasing intensification rate and genesis frequency in some tropical regions conflicted with the impact of thermodynamic variables; rather, they were in accord with the impact of dynamic variables (i.e., vorticity and wind shear). In conclusion, the spatially inhomogeneous trends in TC intensity could be explained by considering the thermodynamic and dynamic aspects in combination through intensification rate and genesis frequency.
On the Relationship between Winter Sea Ice and Summer Atmospheric Circulation over Eurasia
Authors: Wu Bingyi , Zhang Renhe , D'Arrigo Rosanne , Su Jingzhi 
Published: February 2013

Abstract
Using NCEP–NCAR reanalysis and Japanese 25-yr Reanalysis (JRA-25) data, this paper investigates the association between winter sea ice concentration (SIC) in Baffin Bay southward to the eastern coast of Newfoundland, and the ensuing summer atmospheric circulation over the mid- to high latitudes of Eurasia. It is found that winter SIC anomalies are significantly correlated with the ensuing summer 500-hPa height anomalies that dynamically correspond to the Eurasian pattern of 850-hPa wind variability and significantly influence summer rainfall variability over northern Eurasia. Spring atmospheric circulation anomalies south of Newfoundland, associated with persistent winter–spring SIC and a horseshoe-like pattern of sea surface temperature (SST) anomalies in the North Atlantic, act as a bridge linking winter SIC and the ensuing summer atmospheric circulation anomalies over northern Eurasia. Indeed, this study only reveals the association based on observations and simple simulation experiments with SIC forcing. The more precise mechanism for this linkage needs to be addressed in future work using numerical simulations with SIC and SST as the external forcings. The results herein have the following implication: Winter SIC west of Greenland is a possible precursor for summer atmospheric circulation and rainfall anomalies over northern Eurasia.
Extracting the Dominant SST Modes Impacting North America's Observed Climate*
Authors: Wang Fuyao , Liu Zhengyu , Notaro Michael 
Published: February 2013

Abstract
The seasonal impacts of the dominant sea surface temperature (SST) modes to North American climate are assessed comprehensively in observations using the multivariate generalized equilibrium feedback assessment (GEFA) method. The GEFA method is first validated before applying it to observations. Impacts of each individual SST mode are quantified and the associated mechanisms are discussed. Four critical SST modes for North American climate are found: the ENSO mode, Indian Ocean Basin (IOB) mode, North Pacific first empirical orthogonal function (EOF) mode, and tropical Atlantic second EOF mode. The impacts of the ENSO mode are consistent with previous studies qualitatively, while the impact strength is further quantified here. The IOB mode has a strong influence on surface air temperature across North America, and it is demonstrated for the first time that its impact strength might even exceed that of ENSO during both winter and summer. The IOB mode also affects the year-round precipitation. A deeper understanding of the impact of North Pacific SSTs on wintertime surface air temperature is achieved: namely, positive SST anomalies in the Kuroshio Extension region correspond to colder (warmer) air in western (eastern) North America. The tropical Atlantic has a more significant influence on North American precipitation than does the extratropical Atlantic, with colder than normal tropical North Atlantic SSTs supporting wetter conditions across much of the United States, especially during autumn. Because of the linearity of GEFA, the total impacts of multiple SST modes can be obtained by the linear combination of each individual mode's impact. The GEFA method is a potentially powerful tool for seasonal climate prediction.