bluuebunny/crossref_metadata_2025_split huggingface dataset:
“They still phone even though they know I'm deaf”: exploring experiences of deaf people in health services in Wales, UK
Authors: Terry Julia , Meara Rhian , England Rachel 
Published: June 2024

Abstract

Background
Deaf and hard of hearing people persistently experience barriers accessing health services, largely due to ineffective communication systems, a lack of flexible booking arrangements, and a lack of Deaf awareness training for health professional staff.

Methods
Face to face focus groups were conducted with 66 Deaf and hard of hearing people in Deaf clubs across Wales, UK. Thematic analysis was undertaken.

Results
Responses identified from focus groups are reported as barriers faced using health services, improvements that would make a difference, impact of accessibility of health services, and a potential Sign language badge for healthcare staff.

Conclusions
Deaf people report that health professionals lack training on Deaf awareness and do not know how to communicate effectively with Deaf and hard of hearing people. Further research into Deaf awareness and training resources for health professionals are needed to establish what improves Deaf cultural competencies, and ultimately makes healthcare experiences more positive for people who are Deaf.
Zearalenone modulates the function of goat endometrial cells via the mitochondrial quality control system
Authors: Zhang Guomin , Zeng Cheng , Sun Xuan , Zhang Qi , Wang Yifei , Xia Rongxin , Mai Qingyang , Xue Gang , Huang Han , Wang Feng 
Published: June 2024

AbstractZearalenone (ZEN) is a mycotoxin known for its estrogen‐like effects, which can disrupt the normal physiological function of endometrial cells and potentially lead to abortion in female animals. However, the precise mechanism by which ZEN regulates endometrial function remains unclear. In this study, we found that the binding receptor estrogen receptors for ZEN is extensively expressed across various segments of the uterus and within endometrial cells, and a certain concentration of ZEN treatment reduced the proliferation capacity of goat endometrial epithelial cells (EECs) and endometrial stromal cells (ESCs). Meanwhile, cell cycle analysis revealed that ZEN treatment leaded to cell cycle arrest in goat EECs and ESCs. To explore the underlying mechanism, we investigated the mitochondrial quality control systems and observed that ZEN triggered excessive mitochondrial fission and disturbed the balance of mitochondrial fusion‐fission dynamics, impaired mitochondrial biogenesis, increased mitochondrial unfolded protein response and mitophagy in goat EECs and ESCs. Additionally, ZEN treatment reduced the activities of mitochondrial respiratory chain complexes, heightened the production of hydrogen peroxide and reactive oxygen species, and caused cellular oxidative stress and mitochondrial dysfunction. These results suggest that ZEN has adverse effects on goat endometrium cells by disrupting the mitochondrial quality control system and affecting cell cycle and proliferation. Understanding the underlying molecular pathways involved in ZEN‐induced mitochondrial dysfunction and its consequences on cell function will provide critical insights into the reproductive toxicity of ZEN and contribute to safeguarding the health and wellbeing of animals and humans exposed to this mycotoxin.
How Many Imaging Studies Do Patients With Neuromuscular Early Onset Scoliosis (EOS) Receive?
Authors: Lin Adrian J. , Chen Vivian , Wong Cynthis , Tamrazi Benita , Skaggs David L. , Illingworth Kenneth D. , Heffernan Michael J. , Andras Lindsay M. 
Published: June 2024

Background:
Children with neuromuscular early onset scoliosis (EOS) receive numerous radiographic studies both from orthopaedic and other specialties. Ionizing radiation doses delivered by computed tomography (CT) are reportedly 100 times higher than conventional radiography. The purpose of this study was to evaluate the number of radiographic studies ordered for neuromuscular EOS patients during their care.

Methods:
Retrospective review at a tertiary children’s hospital from January 2010 to June 2021 included all patients with neuromuscular EOS followed by an orthopaedic specialist for a minimum of 3 years. Patients were excluded if the majority of their nonorthopaedic care was provided by outside institutions.

Results:
Eighteen patients met inclusion criteria with mean follow up of 6.4±2.3 years. A total of 1312 plain radiographs and 35 CT scans were performed. Of the plain radiographs, 34.7% were ordered by orthopaedic providers and 65.3% (857/1312) were ordered by other providers. Of the CT scans, 4 were ordered by orthopaedic providers, while 88.5% (21/35) were ordered by other providers. An average of 74.7 (range: 29 to 124) radiographs and 1.9 (range: 0 to 9) CT scans ordered over the course of each patient’s treatment for an average of 13.0±6.0 radiographs and 0.3 CT scans per year.

Conclusions:
With an average of 75 radiographs and 1.9 CT scans performed per patient, consideration for steps to limit exposure to ionizing radiation should be made a particularly high priority in this unique subset of patients. This requires interdisciplinary coordination as 65% of the radiographs and over 80% of the CT scans were ordered by nonorthopaedic providers.

Level of Evidence:
Level III.
Cutaneous “Microcystic” Microsecretory Adenocarcinoma With Marked Adnexal Hyperplasia: A Simulant of Microcystic Adnexal Carcinoma
Authors: Novice Taylor , Vellaichamy Gautham , McCalmont Timothy H. , Moesch John 
Published: June 2024

Abstract:
Microsecretory adenocarcinoma (MSA) was first described in 2019 as a low-grade salivary gland neoplasm of intraoral origin with distinct histopathologic features and a characteristic MEF2C::SS18 fusion. Recently, skin was also identified as a primary site for MSA in a report by Bishop et al. Due to its rarity and resemblance to other adnexal tumors, MSA is a challenging diagnosis. Herein, we present a case of cutaneous MSA that was unique for the presence of a significant microcystic component and marked adnexal hyperplasia, which mimicked myxoid microcystic adnexal carcinoma (MAC). A 58-year-old presented with a 1 year history of an enlarging eyelid nodule. Histopathologic analysis revealed dermal tumor composed of small tubules containing inspissated bluish mucinous material. Accompanying marked adnexal hyperplasia and many microcysts were also present. Tumor cells expressed S100 protein, which is distinct from MAC, while p63 immunohistochemistry showed marked loss of myoepithelial labeling, as is common in primary adenocarcinomas. Next generation gene sequencing detected the characteristic MSA fusion protein MEF2c::SS18. We report a novel presentation of MSA that simulated MAC because of the presence of associated microcystic change. The presence of S100 immunopositivity and the identification of MEF2C::SS18 fusion confirmed the diagnosis of cutaneous MSA.
Impact of Illness Uncertainty on Health-Related Quality of Life in Patients With Unruptured Intracranial Aneurysms After Coil Embolization
Authors: Lee JiEun , Lee SukJeong 
Published: June 2024

ABSTRACT

BACKGROUND: This study aimed to identify the impact of illness uncertainty on physical and mental health-related quality of life (HRQoL) in patients with unruptured intracranial aneurysms (UIAs) after coil embolization. METHODS: A cross-sectional study was conducted and HRQoL was evaluated using the Short Form-12 Health Survey Questionnaire, which assesses physical and mental HRQoL. RESULTS: The participants had a mean age of 56.4 years, and among them, 190 (71.2%) were women. Physical and mental HRQoL were negatively correlated with physical symptoms, anxiety, depression, and illness uncertainty. Mental HRQoL was positively correlated with social support. Physical HRQoL was significantly influenced by depression (β = −0.26, P = .004) and was not influenced by illness uncertainty (β = −0.10, P = .101). Mental HRQoL was significantly influenced by anxiety (β = −0.45, P < .001), depression (β = −0.19, P = .003), social support (β = 0.14, P = .004), and illness uncertainty (β = −0.14, P = .005). The finding that illness uncertainty influences HRQoL suggests a basis for interventions aimed at improving mental HRQoL by reducing illness uncertainty in patients with UIAs. CONCLUSION: The physical and mental HRQoL in patients with UIAs after coil embolization is negatively influenced by depression, whereas mental HRQoL is also affected by anxiety, illness uncertainty, and social support. These results may serve as reference data for the design and development of interventions to improve HRQoL in patients with UIAs after coil embolization.
Effects of Exercise on Balance in Cancer Survivors With Chemotherapy-Induced Peripheral Neuropathy
Authors: Khemthong Usa , Hawsawi Samah , Schneider Joanne Kraenzle 
Published: June 2024

Background
Balance impairment in cancer survivors can be a consequence of chemotherapy-induced peripheral neuropathy (CIPN). Previous meta-analyses suggested that exercise significantly improved balance, but the results were only based on 3 and 4 primary studies.

Objectives
This meta-analysis examined the effects of exercise on balance in cancer survivors with CIPN and investigated the moderating effects of source, methods, interventions, and participant characteristics.

Methods
We searched 12 electronic databases and 5 websites without date restriction through December 18, 2023, for primary studies examining the effect of exercise to improve balance in cancer survivors with CIPN reported in English. We retrieved 12 studies that provided 14 comparisons (N = 576), coded them, assessed quality indicators, and evaluated effect sizes across studies.

Results
Using the random-effects model, the estimated summary effect (Hedges’ g) of exercise on balance was 0.68 (Knapp-Hartung adjusted 95% confidence interval, 0.41–0.94; P = .0001) compared with comparison groups. Subgroup analysis revealed that blinded data collectors, intervention fidelity examination, and difference in exercise protocol significantly influenced effect size. Meta-regression analysis showed that session minutes was associated with higher effect sizes.

Conclusion
Exercise with balance training significantly improved balance in cancer survivors. Balance training should be integrated into the current exercise guidelines for cancer survivors with CIPN.

Implications for Practice
Nurses should encourage cancer survivors to engage in exercise with balance training when undergoing chemotherapy. Physical function and barriers to exercise should be assessed before delivering exercise interventions. Exercises should be tailored according to individual performance.
Implementing a Frailty-Specific Postoperative Order Set to Improve Postoperative Outcomes in Frail Adults After Elective Thoracic Surgery
Authors: Allen Amani , Francisco Mary Ann , Jatis Jessica , Turner Yonous , Nordgren Rachel , Rubin Daniel , Huisingh-Scheetz Megan , Bryan Darren S. , Donington Jessica , Ferguson Mark K. , Gleason Lauren J. , Madariaga Maria Lucia 
Published: June 2024

Background:
Frailty is independently associated with adverse patient outcomes after surgery. The current standards of postoperative care rarely consider frailty status.

Local Problem:
There was no standardized protocol to optimize specialized postoperative care for frail patients at an academic medical center.

Methods:
A quasi-experimental pre-/postimplementation study design, using the Reach, Effectiveness, Adoption, Implementation, Maintenance implementation framework, was utilized.

Interventions:
A frailty-specific postoperative order set (FPOS) was developed, including tailored nursing care, activity levels, and nutritional goals.

Results:
There were significant improvements in nurse’s self-reported familiarity with frailty (P = .003) and FPOS awareness (P < .001). The number of orders for delirium prevention, elimination, nutrition, sleep promotion, and sensory support increased (P < .001).

Conclusions:
Implementing an FPOS showed improvements in nurse frailty knowledge, awareness, and order set utilization.
Food and water uptake are regulated by distinct central amygdala circuits revealed using intersectional genetics
Authors: Fermani Federica , Chang Simon , Peters Christian , Gaitanos Louise , Morales Pilar L. Alcala , Ramakrishnan Charu , Deisseroth Karl , Klein Rüdiger 
Published: June 2024

ABSTRACTThe central amygdala (CeA) plays a crucial role in defensive and appetitive behaviours. It contains genetically defined GABAergic neuron subpopulations distributed over three anatomical subregions, capsular (CeC), lateral (CeL), and medial (CeM). The roles that these molecularly- and anatomically-defined CeA neurons play in appetitive behavior remain unclear. Using intersectional genetics, we found that neurons driving food or water consumption are confined to the CeM. Separate CeM subpopulations exist for water only versus water or food consumption.In vivocalcium imaging revealed that CeMHtr2aneurons promoting feeding are responsive towards appetitive cues with little regard for their physical attributes. CeMSstneurons involved in drinking are sensitive to the physical properties of salient stimuli. Both CeM subtypes receive inhibitory input from CeL and send projections to the parabrachial nucleus to promote appetitive behavior. These results suggest that distinct CeM microcircuits evaluate liquid and solid appetitive stimuli to drive the appropriate behavioral responses.
A database of digital line drawings that depict expected and unexpected action-place relationships
Authors: Aydemir Sabiha Tezcan , Umul Seray Tezcan , Urgen Burcu A. 
Published: June 2024

AbstractIn the present study, we introduce an image database created with a simple digital line-drawing tool to represent the expected and unexpected action-place relationships. This database consists of 70 drawings. We validated the dataset with 207 participants. They evaluated the actions, places, and the probability of an action taking place in the respective location. The comprehensibility of each drawing was evaluated using three measures: H-statistics (entropy), which is a measure of the uncertainty of the definition; the definition similarity percentages which is a measure of the naming agreement that is consistent among participants; and the mean probability of the depicted action taking place in the depicted location. Each drawing includes different agents and environments, providing researchers with the opportunity to use this dataset in various fields of cognitive neuroscience, including visual recognition, memory, predictive coding, and novelty detection.
The iMab antibody selectively binds to intramolecular and intermolecular i-motif structures
Authors: Ruggiero Emanuela , Marusic Maja , Zanin Irene , Peña Martinez Cristian David , Plavec Janez , Christ Daniel , Richter Sara N. 
Published: June 2024

ABSTRACTi-Motifs are quadruplex nucleic acid conformations that form in cytosine-rich regions. Because of their acidic pH dependence, iMs were thought to form onlyin vitro. The recent development of an iM-selective antibody, iMab, has allowed iM detection in cells, which revealed their presence at gene promoters and their cell cycle dependence. However, recently evidence emerged which seemed to suggest that iMab recognizes C-rich sequences regardless of their iM conformation. To further investigate the selectivity of iMab, we examined the binding of iMab to C-rich sequences, using a combination of pull-down and Western blot assays. Here we observe that the composition of buffers used during binding and washing steps strongly influences the selectivity of antibody binding. In addition, we demonstrate by NMR that several of the previously reported C-rich sequences, which were not expected to form iMs, actually formintermoleculariMs which are selectively recognized by iMab. Our results highlight the specificity of the iMab antibody, emphasize the importance of optimizing DNA concentrations, blocking and washing conditions, and confirm iMab selectivity not only for intramolecular iMs, but also for intermolecular iMs.
Nanoparticles co-delivering siRNA and mRNA for simultaneous restoration and silencing of gene/protein expressionin vitroandin vivo
Authors: Manturthi Shireesha , El-Sahli Sara , Bo Yuxia , Durocher Emma , Kirkby Melanie , Popatia Alyanna , Mediratta Karan , Daniel Redaet , Lee Seung-Hwan , Iqbal Umar , Côté Marceline , Wang Lisheng , Gadde Suresh 
Published: June 2024

AbstractRNA-based agents such as siRNA, miRNA, and mRNA can selectively manipulate gene expression/proteins and have the potential to revolutionize the current therapeutic strategies for various diseases, including cancer. To address the poor stability and inherent limitations of RNA agents, nanoparticle (NP) platforms have been developed to deliver functional mRNA or siRNA inside the cells. Recent studies have focused on either siRNA to knock down proteins causing drug resistance or mRNA technology to introduce tumor suppressors. However, complex diseases like cancer need multi-targeted approaches to selectively target multiple gene expressions/proteins. In this proof-of-concept study, we developed co-delivery nanoparticles containing Luc-mRNA and siRNA-GFP as model RNA agents ((M+S)-NPs) and assessed their effectsin vitroandin vivo. Our studies show that NPs can effectively deliver both functional mRNA and siRNA together, simultaneously impacting the expression of two genes/proteinsin vitro. Additionally, afterin vivoadministration, co-delivery NPs successfully knocked down GFP while introducing luciferase in a TNBC mouse model, indicating our NPs have the potential to develop RNA-based anticancer therapeutics. These studies pave the way to develop RNA-based, multitargeted, multi-delivery approaches for complex diseases like cancer.TOC
Restoring hippocampal glucose metabolism rescues cognition across Alzheimer’s disease pathologies
Authors: Minhas Paras S. , Jones Jeffrey R. , Latif-Hernandez Amira , Sugiura Yuki , Durairaj Aarooran S. , Uenaka Takeshi , Wang Qian , Mhatre Siddhita D. , Liu Ling , Conley Travis , Ennerfelt Hannah , Jung Yoo Jin , Prasad Praveena , Jenkins Brenita C. , Goodman Ryan , Newmeyer Traci , Heard Kelly , Kang Austin , Wilson Edward N. , Ullian Erik M. , Serrano Geidy E. , Beach Thomas G. , Rabinowitz Joshua D. , Wernig Marius , Suematsu Makoto , Longo Frank M. , McReynolds Melanie R. , Gage Fred H. , Andreasson Katrin I. 
Published: June 2024

ABSTRACTImpaired cerebral glucose metabolism is a pathologic feature of Alzheimer Disease (AD), and recent proteomic studies highlight a disruption of glial carbohydrate metabolism with disease progression. Here, we report that inhibition of indoleamine-2,3-dioxygenase 1 (IDO1), which metabolizes tryptophan to kynurenine (KYN) in the first step of the kynurenine pathway, rescues hippocampal memory function and plasticity in preclinical models of amyloid and tau pathology by restoring astrocytic metabolic support of neurons. Activation of IDO1 in astrocytes by amyloid-beta42and tau oligomers, two major pathological effectors in AD, increases KYN and suppresses glycolysis in an AhR-dependent manner. Conversely, pharmacological IDO1 inhibition restores glycolysis and lactate production. In amyloid-producingAPPSwe-PS1ΔE9and 5XFAD mice and in tau-producing P301S mice, IDO1 inhibition restores spatial memory and improves hippocampal glucose metabolism by metabolomic and MALDI-MS analyses. IDO1 blockade also rescues hippocampal long-term potentiation (LTP) in a monocarboxylate transporter (MCT)-dependent manner, suggesting that IDO1 activity disrupts astrocytic metabolic support of neurons. Indeed, in vitro mass-labeling of human astrocytes demonstrates that IDO1 regulates astrocyte generation of lactate that is then taken up by human neurons. In co-cultures of astrocytes and neurons derived from AD subjects, deficient astrocyte lactate transfer to neurons was corrected by IDO1 inhibition, resulting in improved neuronal glucose metabolism. Thus, IDO1 activity disrupts astrocytic metabolic support of neurons across both amyloid and tau pathologies and in a model of AD iPSC-derived neurons. These findings also suggest that IDO1 inhibitors developed for adjunctive therapy in cancer could be repurposed for treatment of amyloid- and tau-mediated neurodegenerative diseases.
ColocZStats: A Z-Stack Signal Colocalization Extension Tool for 3D Slicer
Authors: Chen Xiang , Thakur Teena , Jeyasekharan Anand D. , Benoukraf Touati , Meruvia-Pastor Oscar 
Published: June 2024

ABSTRACTConfocal microscopy has evolved as a widely adopted imaging technique in molecular biology and is frequently utilized to achieve accurate subcellular localization of proteins. Applying colocalization analysis on image z-stacks obtained from confocal fluorescence microscopes is a dependable method to reveal the association between different molecules. In addition, despite the established advantages and growing adoption of 3D visualization software in various microscopy research domains, there has been a scarcity of systems supporting colocalization analysis within a user-specified region of interest (ROI). In this context, several broadly employed biological image visualization platforms were meticulously explored in this study to comprehend the current landscape. It has been observed that while these applications can generate three-dimensional (3D) reconstructions for the z-stacks and in some cases transfer them into an immersive Virtual Reality (VR) scene, there is still a lack of support for performing quantitative colocalization analysis on such images based on a user-defined ROI and thresholding levels. To address these issues, an extension called ColocZStats has been developed for 3D Slicer, a widely used free and open-source software package for image analysis and scientific visualization. With a custom-designed user-friendly interface, ColocZStats allows investigators to conduct intensity thresholding and ROI selection on imported 3D image stacks. It can deliver several essential colocalization metrics for structures of interest and produce reports in the form of diagrams and spreadsheets.
Out-of-Anatolia: cultural and genetic interactions during the Neolithic expansion in the Aegean
Authors: Koptekin Dilek , Aydoğan Ayça , Karamurat Cansu , Altınışık N. Ezgi , Vural Kıvılcım Başak , Kazancı D. Deniz , Doğu Ayça Küçükakdağ , Kaptan Damla , Gemici Hasan Can , Yüncü Eren , Umurtak Gülsün , Duru Refik , Fidan Erkan , Çevik Özlem , Erdoğu Burçin , Korkut Taner , Knüsel Christopher J. , Haddow Scott , Larsen Clark Spencer , Özbal Rana , Gerritsen Fokke , Özdoğan Eylem , Usanmaz Uygar Ozan , Derici Yasin Cemre , Uçmazoğlu Mine , Jay Flora , Özdoğan Mehmet , Götherström Anders , Erdal Yılmaz Selim , Malaspinas Anna-Sapfo , Atakuman Çiğdem , Özer Füsun , Somel Mehmet 
Published: June 2024

AbstractWestern Anatolia has been a crucial yet elusive element in the Neolithic expansion from the Fertile Crescent to Europe. Using 30 new palaeogenomes from Anatolia c.8000-6000 BCE we describe the early Holocene genetic landscape of Western Anatolia, which reveals population continuity since the late Upper Pleistocene. Our findings indicate that the Neolithisation of Western Anatolia in the 7thmillennium BCE was a multifaceted process, characterised by the assimilation of Neolithic practices by indigenous groups and the influx of populations from the east, their admixed descendants eventually laying the foundations of Neolithic Southeast Europe. Intriguingly, the observed diversity in material culture among Aegean Early Neolithic communities correlates with their geographical distances but not their genetic differences, signifying a decoupling between cultural developments and genetic admixture processes.