bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of Psychological Entitlement in 28 Countries
Authors: Żemojtel-Piotrowska Magdalena A. , Piotrowski Jarosław P. , Cieciuch Jan , Calogero Rachel M. , Van Hiel Alain , Argentero Piergiorgio , Baltatescu Sergiu , Baran Tomasz , Bardhwaj Gopa , Bukowski Marcin , Chargazia Melania , Clinton Amanda , Halik Murnizam H. J. , Ilisko Dzintra , Khachatryan Narine , Klicperova-Baker Martina , Kostal Jaroslav , Kovacs Monika , Letovancova Eva , Liik Kadi , Marganski Alison , Michalowski Jaroslaw , Nord Iwo , Paspalanova Elena , Perez de Leon Pablo , Techera José , Rojas Mariano , Rozycka Joanna , Sawicka Aleksandra , Seibt Beate , Semkiv Iryna , Tiliouine Habib , Khanh Truong Ha , van den Bos Kees , Wills-Herrera Eduardo 
Published: October 2015

Abstract. This article presents the cross-cultural validation of the Entitlement Attitudes Questionnaire, a tool designed to measure three facets of psychological entitlement: active, passive, and revenge entitlement. Active entitlement was defined as the tendency to protect individual rights based on self-worthiness. Passive entitlement was defined as the belief in obligations to and expectations toward other people and institutions for the fulfillment of the individual’s needs. Revenge entitlement was defined as the tendency to protect one’s individual rights when violated by others and the tendency to reciprocate insults. The 15-item EAQ was validated in a series of three studies: the first one on a general Polish sample (N = 1,900), the second one on a sample of Polish students (N = 199), and the third one on student samples from 28 countries (N = 5,979). A three-factor solution was confirmed across all samples. Examination of measurement equivalence indicated partial metric invariance of EAQ for all national samples. Discriminant and convergent validity of the EAQ was also confirmed.
Confirmatory Factor Analysis of the Gotland Male Depression Scale in an Australian Community Sample
Authors: Rice Simon M. , Aucote Helen M. , Möller-Leimkühler Anne Maria , Amminger G. Paul 
Published: October 2015

Abstract. The Gotland Male Depression Scale (GMDS) was developed to improve the assessment and identification of depression in men by assessing symptoms that fall outside DSM-V/ICD-10 diagnostic criteria for depression. However, research findings from the GMDS have been markedly discrepant. Attempts to validate the latent GMDS factor structure using exploratory factor analysis (EFA) have yielded inconsistent results, bringing the validity and public health utility of the scale into question. The current study used confirmatory factor analysis to test the construct validity of five competing latent factor models identified from prior research. Data were obtained and analyzed separately from an Australian community sample of adult males (n = 318) and females (n = 345). Fit indices demonstrated the original GMDS two-factor model (distress, depression) to be a poor fit to the data. An alternative mixed three-factor model demonstrated improved model fit, although indices remained marginal. Results question the factor structure validity of the GMDS in the present sample and highlight the need for further psychometric development of the scale.
The Self-Control Cognition Questionnaire
Authors: Maas Joyce , Keijsers Ger P. J. , Cangliosi Claudia M. , van der Veld William , Tanis-Jacobs Jorg , van Minnen Agnes 
Published: October 2015

Abstract. Self-control cognitions arise right before or after someone gives in to an unwanted habit. This paper reports on the development of the 11-item Self-Control Cognition Questionnaire (SCCQ) in a series of three studies. In the first study (N = 308), we found that the SCCQ has a two-factor structure and is reliable. The factors were named “Giving way is rewarding” and “Resistance is impossible.” The construct validity of the SCCQ was assessed in the second study (N = 138). As expected, the SCCQ correlated positively and strongly with the preoccupation with unwanted habits and with the experience of craving, and correlations with one’s tendency to consider the long-term consequences of actions were small. The third study demonstrated that the SCCQ discriminates between patients with habit disorders (N = 63) and controls with non-pathological unwanted habits (N = 106). The SCCQ was sensitive to therapeutic change in two patient samples, one suffering from hair pulling disorder and the other from pathological skin picking. The SCCQ is applicable to unwanted habits in general, both pathological and non-pathological. It is proven to have sound psychometric properties and is suitable for use in practice.
Resident c-kit+ cells in the heart are not cardiac stem cells
Authors: Sultana Nishat , Zhang Lu , Yan Jianyun , Chen Jiqiu , Cai Weibin , Razzaque Shegufta , Jeong Dongtak , Sheng Wei , Bu Lei , Xu Mingjiang , Huang Guo-Ying , Hajjar Roger J. , Zhou Bin , Moon Anne , Cai Chen-Leng 
Published: October 2015

AbstractIdentifying abona fidepopulation of cardiac stem cells (CSCs) is a critical step for developing cell-based therapies for heart failure patients. Previously, cardiac c-kit+cells were reported to be CSCs with a potential to become myocardial, endothelial and smooth muscle cellsin vitroand after cardiac injury. Here we provide further insights into the nature of cardiac c-kit+cells. By targeting thec-kitlocus with multiple reporter genes in mice, we find thatc-kitexpression rarely co-localizes with the expression of the cardiac progenitor and myogenic markerNkx2.5, or that of the myocardial marker, cardiactroponin T (cTnT). Instead, c-kit predominantly labels a cardiac endothelial cell population in developing and adult hearts. After acute cardiac injury, c-kit+cells retain their endothelial identity and do not become myogenic progenitors or cardiomyocytes. Thus, our work strongly suggests that c-kit+cells in the murine heart are endothelial cells and not CSCs.
Genetic pathways for differentiation of the peripheral nervous system in ascidians
Authors: Waki Kana , Imai Kaoru S. , Satou Yutaka 
Published: October 2015

AbstractAscidians belong to tunicates, the sister group of vertebrates. Peripheral nervous systems (PNSs) including epidermal sensory neurons (ESNs) in the trunk and dorsal tail regions of ascidian larvae are derived from cells adjacent to the neural plate, as in vertebrates. On the other hand, peripheral ESNs in the ventral tail region are derived from the ventral ectoderm under the control of BMP signalling, reminiscent of sensory neurons of amphioxus and protostomes. In this study, we show that two distinct mechanisms activate a common gene circuit consisting of Msx, Ascl.b, Tox, Delta.b and Pou4 in the dorsal and ventral regions to differentiate ESNs. Our results suggest that ventral ESNs of the ascidian larva are not directly homologous to vertebrate PNSs. The dorsal ESNs might have arisen via co-option of the original PNS gene circuit to the neural plate border in an ancestral chordate.
Predicting the behavior of microfluidic circuits made from discrete elements
Authors: Bhargava Krisna C. , Thompson Bryant , Iqbal Danish , Malmstadt Noah 
Published: October 2015

AbstractMicrofluidic devices can be used to execute a variety of continuous flow analytical and synthetic chemistry protocols with a great degree of precision. The growing availability of additive manufacturing has enabled the design of microfluidic devices with new functionality and complexity. However, these devices are prone to larger manufacturing variation than is typical of those made with micromachining or soft lithography. In this report, we demonstrate a design-for-manufacturing workflow that addresses performance variation at the microfluidic element and circuit level, in context of mass-manufacturing and additive manufacturing. Our approach relies on discrete microfluidic elements that are characterized by their terminal hydraulic resistance and associated tolerance. Network analysis is employed to construct simple analytical design rules for model microfluidic circuits. Monte Carlo analysis is employed at both the individual element and circuit level to establish expected performance metrics for several specific circuit configurations. A protocol based on osmometry is used to experimentally probe mixing behavior in circuits in order to validate these approaches. The overall workflow is applied to two application circuits with immediate use at on the bench-top: series and parallel mixing circuits that are modularly programmable, virtually predictable, highly precise and operable by hand.
Enhanced ethanol production via electrostatically accelerated fermentation of glucose using Saccharomyces cerevisiae
Authors: Mathew Anup Sam , Wang Jiapeng , Luo Jieling , Yau Siu-Tung 
Published: October 2015

AbstractThe global demand for ethanol as an alternative fuel continues to rise. Advancement in all aspects of ethanol production is deemed beneficial to the ethanol industry. Traditional fermentation requires 50–70 hours to produce the maximum ethanol concentration of 7–8% (v/v). Here we demonstrate an electrostatic fermentation method that is capable of accelerating the fermentation of glucose using generic Saccharomyces cerevisiae as the fermenting microorganism to produce ethanol. The method, when applied to the batch fermentation of 1 liter fermenting mixture containing dry yeast without pre-culture, is able to achieve ethanol yield on the high gravity level (12.3% v/v) in 24 hours. The fermentation results in almost complete consumption of glucose. With pre-cultured yeast, ethanol yield can reach 14% v/v in 20 hours. The scale-up capability of the method is demonstrated with 2 liter fermenting mixture. The method does not consume external energy due to its electrostatic nature. Our results indicate the applicability of the fermentation technique to industry applications.
Complex stability and dynamic subunit interchange modulates the disparate activities of the yeast moonlighting proteins Hal3 and Vhs3
Authors: Abrie J. Albert , Molero Cristina , Ariño Joaquín , Strauss Erick 
Published: October 2015

AbstractSaccharomyces cerevisiae Hal3 and Vhs3 are moonlighting proteins, acting both as inhibitors of the serine/threonine protein phosphatase Ppz1 and as subunits (together with Cab3) of the unique heterotrimeric phosphopantothenoylcysteine decarboxylase (PPCDC) enzyme of Hemiascomycetous yeast. Both these roles are essential: PPCDC catalyses the third step of coenzyme A biosynthesis, while Ppz1 inhibition is required for regulation of monovalent cation homeostasis. However, the mechanisms by which these proteins’ disparate activities are regulated are not well understood. The PPCDC domains (PDs) of Hal3, Vhs3 and Cab3 constitute the minimum requirement for these proteins to show both PPCDC activity and, in the case of Hal3 and Vhs3, to bind to Ppz1. Using these PD proteins as a model system to study the possibility of dynamic interchange between these roles, we provide evidence that Hal3 binds Ppz1 as a monomer (1:1 stoichiometry), requiring it to de-oligomerize from its usual homo- and heterotrimeric states (the latter having PPCDC activity). This de-oligomerization is made possible by structural features that set Hal3 apart from Vhs3, increasing its ability to undergo monomer exchange. These findings suggest that oligomer interchange may be a significant factor in the functional regulation of these proteins and their various unrelated (moonlighting) functions.
Ultrafast traveling wave dominates the electric organ discharge of Apteronotus leptorhynchus: an inverse modelling study
Authors: Shifman Aaron R. , Longtin André , Lewis John E. 
Published: October 2015

AbstractIdentifying and understanding the current sources that give rise to bioelectric fields is a fundamental problem in the biological sciences. It is very difficult, for example, to attribute the time-varying features of an electroencephalogram recorded from the head surface to the neural activity of specific brain areas; model systems can provide important insight into such problems. Some species of fish actively generate an oscillating (c. 1000 Hz) quasi-dipole electric field to communicate and sense their environment in the dark. A specialized electric organ comprises neuron-like cells whose collective signal underlies this electric field. As a step towards understanding the detailed biophysics of signal generation in these fish, we use an anatomically-detailed finite-element modelling approach to reverse-engineer the electric organ signal over one oscillation cycle. We find that the spatiotemporal profile of current along the electric organ constitutes a travelling wave that is well-described by two spatial Fourier components varying in time. The conduction velocity of this wave is faster than action potential conduction in any known neuronal axon (>200 m/s), suggesting that the spatiotemporal features of high-frequency electric organ discharges are not constrained by the conduction velocities of spinal neuron pathways.
Dental enamel cells express functional SOCE channels
Authors: Nurbaeva Meerim K. , Eckstein Miriam , Concepcion Axel R. , Smith Charles E. , Srikanth Sonal , Paine Michael L. , Gwack Yousang , Hubbard Michael J. , Feske Stefan , Lacruz Rodrigo S. 
Published: October 2015

AbstractDental enamel formation requires large quantities of Ca2+ yet the mechanisms mediating Ca2+ dynamics in enamel cells are unclear. Store-operated Ca2+ entry (SOCE) channels are important Ca2+ influx mechanisms in many cells. SOCE involves release of Ca2+ from intracellular pools followed by Ca2+ entry. The best-characterized SOCE channels are the Ca2+ release-activated Ca2+ (CRAC) channels. As patients with mutations in the CRAC channel genes STIM1 and ORAI1 show abnormal enamel mineralization, we hypothesized that CRAC channels might be an important Ca2+ uptake mechanism in enamel cells. Investigating primary murine enamel cells, we found that key components of CRAC channels (ORAI1, ORAI2, ORAI3, STIM1, STIM2) were expressed and most abundant during the maturation stage of enamel development. Furthermore, inositol 1,4,5-trisphosphate receptor (IP3R) but not ryanodine receptor (RyR) expression was high in enamel cells suggesting that IP3Rs are the main ER Ca2+ release mechanism. Passive depletion of ER Ca2+ stores with thapsigargin resulted in a significant raise in [Ca2+]i consistent with SOCE. In cells pre-treated with the CRAC channel blocker Synta-66 Ca2+ entry was significantly inhibited. These data demonstrate that enamel cells have SOCE mediated by CRAC channels and implicate them as a mechanism for Ca2+ uptake in enamel formation.
Modeling the effect of comprehensive interventions on Ebola virus transmission
Authors: Shen Mingwang , Xiao Yanni , Rong Libin 
Published: October 2015

AbstractSince the re-emergence of Ebola in West Africa in 2014, comprehensive and stringent interventions have been implemented to decelerate the spread of the disease. The effectiveness of interventions still remains unclear. In this paper, we develop an epidemiological model that includes various controlling measures to systematically evaluate their effects on the disease transmission dynamics. By fitting the model to reported cumulative cases and deaths in Guinea, Sierra Leone and Liberia until March 22, 2015, we estimate the basic reproduction number in these countries as 1.2552, 1.6093 and 1.7994, respectively. Model analysis shows that there exists a threshold of the effectiveness of isolation, below which increasing the fraction of latent individuals diagnosed prior to symptoms onset or shortening the duration between symptoms onset and isolation may lead to more Ebola infection. This challenges an existing view. Media coverage plays a substantial role in reducing the final epidemic size. The response to reported cumulative infected cases and deaths may have a different effect on the epidemic spread in different countries. Among all the interventions, we find that shortening the duration between death and burial and improving the effectiveness of isolation are two effective interventions for controlling the outbreak of Ebola virus infection.
Midgut of the non-hematophagous mosquito Toxorhynchites theobaldi (Diptera, Culicidae)
Authors: Godoy Raquel S. M. , Fernandes Kenner M. , Martins Gustavo F. 
Published: October 2015

AbstractIn most mosquito species, the females require a blood-feeding for complete egg development. However, inToxorhynchitesmosquitoes, the eggs develop without blood-feeding and both females and males exclusively feed on sugary diets. The midgut is a well-understood organ in blood-feeding mosquitoes, but little is known about it in non-blood-feeding ones. In the present study, the detailed morphology of the midgut ofToxorhynchites theobaldiwere investigated using histochemical and ultrastructural methods. The midgut of female and maleT. theobaldiadults consists of a long, slender anterior midgut (AMG) and a short, dilated posterior midgut (PMG). The AMG is subdivided into AMG1 (short, with folds) and AMG2 (long, without folds). Nerve branches and enteroendocrine cells are present in AMG and PMG, respectively. Compared with the PMG of blood-feeding female mosquitoes, the PMG ofT. theobaldiis smaller; however, in both mosquitoes, PMG seems be the main region of food digestion and absorption and protein secretion. The epithelial folds present in the AMG ofT. theobaldihave not been reported in other mosquitoes; however, the midgut muscle organization and endocrine control of the digestion process are conserved in bothT. theobaldiand blood-feeding mosquitoes.
Littoral lichens as a novel source of potentially bioactive Actinobacteria
Authors: Parrot Delphine , Antony-Babu Sanjay , Intertaglia Laurent , Grube Martin , Tomasi Sophie , Suzuki Marcelino T. 
Published: October 2015

AbstractCultivable Actinobacteria are the largest source of microbially derived bioactive molecules. The high demand for novel antibiotics highlights the need for exploring novel sources of these bacteria. Microbial symbioses with sessile macro-organisms, known to contain bioactive compounds likely of bacterial origin, represent an interesting and underexplored source of Actinobacteria. We studied the diversity and potential for bioactive-metabolite production of Actinobacteria associated with two marine lichens (Lichina confinis and L. pygmaea; from intertidal and subtidal zones) and one littoral lichen (Roccella fuciformis; from supratidal zone) from the Brittany coast (France), as well as the terrestrial lichen Collema auriforme (from a riparian zone, Austria). A total of 247 bacterial strains were isolated using two selective media. Isolates were identified and clustered into 101 OTUs (98% identity) including 51 actinobacterial OTUs. The actinobacterial families observed were: Brevibacteriaceae, Cellulomonadaceae, Gordoniaceae, Micrococcaceae, Mycobacteriaceae, Nocardioidaceae, Promicromonosporaceae, Pseudonocardiaceae, Sanguibacteraceae and Streptomycetaceae. Interestingly, the diversity was most influenced by the selective media rather than lichen species or the level of lichen thallus association. The potential for bioactive-metabolite biosynthesis of the isolates was confirmed by screening genes coding for polyketide synthases types I and II. These results show that littoral lichens are a source of diverse potentially bioactive Actinobacteria.
Immunoproteasome subunit LMP7 Deficiency Improves Obesity and Metabolic Disorders
Authors: Kimura Hiroaki , Usui Fumitake , Karasawa Tadayoshi , Kawashima Akira , Shirasuna Koumei , Inoue Yoshiyuki , Komada Takanori , Kobayashi Motoi , Mizushina Yoshiko , Kasahara Tadashi , Suzuki Koichi , Iwasaki Yusaku , Yada Toshihiko , Caturegli Patrizio , Takahashi Masafumi 
Published: October 2015

AbstractInflammation plays an important role in the development of obesity and metabolic disorders; however, it has not been fully understood how inflammation occurs and is regulated in their pathogenesis. Low-molecular mass protein-7 (LMP7) is a proteolytic subunit of the immunoproteasome that shapes the repertoire of antigenic peptides on major histocompatibility complex class I molecule. In this study, we investigated the role of LMP7 in the development of obesity and metabolic disorders using LMP7-deficient mice. LMP7 deficiency conveyed resistant to obesity and improved glucose intolerance and insulin sensitivity in mice fed with high-fat diet (HFD). LMP7 deficiency decreased pancreatic lipase expression, increased fecal lipid contents and inhibited the increase of plasma triglyceride levels upon oral oil administration or HFD feeding. Using bone marrow-transferred chimeric mice, we found that LMP7 in both bone marrow- and non-bone marrow-derived cells contributes to the development of HFD-induced obesity. LMP7 deficiency decreased inflammatory responses such as macrophage infiltration and chemokine expression while it increased serum adiponection levels. These findings demonstrate a novel role for LMP7 and provide new insights into the mechanisms underlying inflammation in the pathophysiology of obesity and metabolic disorders.
The evolution of tumor metastasis during clonal expansion with alterations in metastasis driver genes
Authors: Yamamoto Kimiyo N. , Nakamura Akira , Haeno Hiroshi 
Published: October 2015

AbstractMetastasis is a leading cause of cancer-related deaths. Carcinoma generally initiates at a specific organ as a primary tumor, but eventually metastasizes and forms tumor sites in other organs. In this report, we developed a mathematical model of cancer progression with alterations in metastasis-related genes. In cases in which tumor cells acquire metastatic ability through two steps of genetic alterations, we derive formulas for the probability, the expected number and the distribution of the number of metastases. Moreover, we investigate practical pancreatic cancer disease progression in cases in which both one and two steps of genetic alterations are responsible for metastatic formation. Importantly, we derive a mathematical formula for the survival outcome validated using clinical data as well as direct simulations. Our model provides theoretical insights into how invisible metastases distribute upon diagnosis with respect to growth rates, (epi)genetic alteration rates, metastatic rate and detection size. Prediction of survival outcome using the formula is of clinical importance in terms of determining therapeutic strategies.
A DNA Mini-Barcoding System for Authentication of Processed Fish Products
Authors: Shokralla Shadi , Hellberg Rosalee S. , Handy Sara M. , King Ian , Hajibabaei Mehrdad 
Published: October 2015

AbstractSpecies substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences—mini-barcodes—for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species commonly used in processed fish products in North America. Six mini-barcode primer pairs targeting short (127–314 bp) fragments of the cytochrome c oxidase I (CO1) DNA barcode region were developed by examining over 8,000 DNA barcodes from species in the U.S. Food and Drug Administration (FDA) Seafood List. The mini-barcode primer pairs were then tested against 44 processed fish products representing a range of species and product types. Of the 44 products, 41 (93.2%) could be identified at the species or genus level. The greatest mini-barcoding success rate found with an individual primer pair was 88.6% compared to 20.5% success rate achieved by the full-length DNA barcode primers. Overall, this study presents a mini-barcoding system that can be used to identify a wide range of fish species in commercial products and may be utilized in high throughput DNA sequencing for authentication of heavily processed fish products.