bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequence of a Foot-and-Mouth Disease Virus of Serotype A Isolated from Vietnam in 2013
Authors: Hwang Ji-Hyeon , Nguyen Tho Dang , Mai Duoung Thuy , Kim Su-Mi , Park Jong-Hyeon , Kim Byounghan , To Thanh Long , Lee Kwang-Nyeong 
Published: September 2015

ABSTRACT
The complete genome sequence of a foot-and-mouth disease virus (FMDV) found in an isolate collected in northern Vietnam in 2013 appears to be closely related to a genetic cluster formed with isolates from China, Mongolia, and Russia in 2013. All of these are classified to fall within the Sea-97 lineage, for which little complete genome data are available.
Complete Genome Sequence of the Type Strain Corynebacterium epidermidicanis DSM 45586, Isolated from the Skin of a Dog Suffering from Pruritus
Authors: Rückert Christian , Eimer Janine , Winkler Anika , Tauch Andreas 
Published: September 2015

ABSTRACT

The complete genome sequence of
Corynebacterium epidermidicanis
DSM 45586 comprises 2,692,072 bp with 58.06% G+C content. The annotation revealed 2,466 protein-coding regions, including genes for surface-anchored proteins with Cna B-type or bacterial Ig-like domains and for an adhesive SpaABC-type pilus with similarity to fimbrial subunits of
Corynebacterium resistens
DSM 45100.
Genome Sequences of Two Multidrug-Resistant Acinetobacter baumannii Clinical Strains Isolated from Southern India
Authors: Balaji Veeraraghavan , Rajenderan Sangeetha , Anandan Shalini , Biswas Indranil 
Published: September 2015

ABSTRACT

Acinetobacter baumannii
is an emerging nosocomial pathogen causing infections worldwide. In this study, we determined the genome sequences of two multidrug-resistant
A. baumannii
clinical strains isolated from a hospital in southern India. Genome analyses indicate that both the strains harbor numerous horizontally transferred genetic elements and antibiotic resistance cassettes.
Complete Genome Sequence and Methylome of Staphylococcus schleiferi, an Important Cause of Skin and Ear Infections in Veterinary Medicine
Authors: Misic Ana M. , Cain Christine L. , Morris Daniel O. , Rankin Shelley C. , Beiting Daniel P. 
Published: September 2015

ABSTRACT

Staphylococcus schleiferi
, a Gram-positive and coagulase-variable organism, is an opportunistic human pathogen and a major cause of skin and soft tissue infections in dogs. Here, we report the first
S. schleiferi
genome sequence and methylome from four canine clinical isolates.
Complete Genome Sequence of Papaya Ringspot Virus Isolated from Genetically Modified Papaya in Hainan Island, China
Authors: Zhao Guangyuan , Yan Pu , Shen Wentao , Tuo Decai , Li Xiaoying , Zhou Peng 
Published: September 2015

ABSTRACT
The complete genome sequence (10,326 nucleotides) of a papaya ringspot virus isolate infecting genetically modified papaya in Hainan Island of China was determined through reverse transcription (RT)-PCR. The virus shares 92% nucleotide sequence identity with the isolate that is unable to infect PRSV-resistant transgenic papaya.
Cancer Stem Cell Marker Phenotypes Are Reversible and Functionally Homogeneous in a Preclinical Model of Pancreatic Cancer
Authors: Dosch Joseph S. , Ziemke Elizabeth K. , Shettigar Amrith , Rehemtulla Alnawaz , Sebolt-Leopold Judith S. 
Published: September 2015

Abstract
Survival rates associated with pancreatic cancer remain dismal despite advancements in detection and experimental treatment strategies. Genetically engineered mouse models of pancreatic tumorigenesis have gained considerable attention based on their ability to recapitulate key clinical features of human disease including chemotherapeutic resistance and fibrosis. However, it is unclear if transgenic systems exemplified by the KrasG12D/Trp53R172H/Pdx-1-Cre (KPC) mouse model recapitulate the functional heterogeneity of human pancreatic tumors harboring distinct cells with tumorigenic properties. To facilitate tracking of heterogeneous tumor cell populations, we incorporated a luciferase-based tag into the genetic background of the KPC mouse model. We isolated pancreatic cancer cells from multiple independent tumor lines and found that roughly 1 out of 87 cells exhibited tumorigenic capability. Notably, this frequency is significantly higher than reported for human pancreatic adenocarcinomas. Cancer stem cell (CSC) markers, including CD133, CD24, Sca-1, and functional Aldefluor activity, were unable to discriminate tumorigenic from nontumorigenic cells in syngeneic transplants. Furthermore, three-dimensional spheroid cultures originating from KPC tumors did not enrich for cells with stem-like characteristics and were not significantly more tumorigenic than cells cultured as monolayers. Additionally, we did not observe significant differences in response to gemcitabine or salinomycin in several isolated subpopulations. Taken together, these studies show that the hierarchical organization of CSCs in human disease is not recapitulated in a commonly used mouse model of pancreatic cancer and therefore provide a new view of the phenotypic and functional heterogeneity of tumor cells. Cancer Res; 75(21); 4582–92. ©2015 AACR.
Mitochondrial Superoxide Dismutase Has a Protumorigenic Role in Ovarian Clear Cell Carcinoma
Authors: Hemachandra L.P. Madhubhani P. , Shin Dong-Hui , Dier Usawadee , Iuliano James N. , Engelberth Sarah A. , Uusitalo Larissa M. , Murphy Susan K. , Hempel Nadine 
Published: September 2015

Abstract
Epithelial ovarian cancer (EOC) is the fourth leading cause of death due to cancer in women and comprises distinct histologic subtypes, which vary widely in their genetic profiles and tissues of origin. It is therefore imperative to understand the etiology of these distinct diseases. Ovarian clear cell carcinoma (OCCC), a very aggressive subtype, comprises &gt;10% of EOCs. In the present study, we show that mitochondrial superoxide dismutase (Sod2) is highly expressed in OCCC compared with other EOC subtypes. Sod2 is an antioxidant enzyme that converts highly reactive superoxide (O2•−) to hydrogen peroxide (H2O2) and oxygen (O2), and our data demonstrate that Sod2 is protumorigenic and prometastatic in OCCC. Inhibiting Sod2 expression reduces OCCC ES-2 cell tumor growth and metastasis in a chorioallantoic membrane (CAM) model. Similarly, cell proliferation, migration, spheroid attachment and outgrowth on collagen, and Akt phosphorylation are significantly decreased with reduced expression of Sod2. Mechanistically, we show that Sod2 has a dual function in supporting OCCC tumorigenicity and metastatic spread. First, Sod2 maintains highly functional mitochondria, by scavenging O2•−, to support the high metabolic activity of OCCC. Second, Sod2 alters the steady-state ROS balance to drive H2O2-mediated migration. While this higher steady-state H2O2 drives prometastatic behavior, it also presents a doubled-edged sword for OCCC, as it pushed the intracellular H2O2 threshold to enable more rapid killing by exogenous sources of H2O2. Understanding the complex interaction of antioxidants and ROS may provide novel therapeutic strategies to pursue for the treatment of this histologic EOC subtype. Cancer Res; 75(22); 4973–84. ©2015 AACR.
Combining miR-10b–Targeted Nanotherapy with Low-Dose Doxorubicin Elicits Durable Regressions of Metastatic Breast Cancer
Authors: Yoo Byunghee , Kavishwar Amol , Ross Alana , Wang Ping , Tabassum Doris P. , Polyak Kornelia , Barteneva Natalia , Petkova Victoria , Pantazopoulos Pamela , Tena Aseda , Moore Anna , Medarova Zdravka 
Published: September 2015

Abstract
The therapeutic promise of microRNA (miRNA) in cancer has yet to be realized. In this study, we identified and therapeutically exploited a new role for miR-10b at the metastatic site, which links its overexpression to tumor cell viability and proliferation. In the protocol developed, we combined a miR-10b–inhibitory nanodrug with low-dose anthracycline to achieve complete durable regressions of metastatic disease in a murine model of metastatic breast cancer. Mechanistic investigations suggested a potent antiproliferative, proapoptotic effect of the nanodrug in the metastatic cells, potentiated by a cell-cycle arrest produced by administration of the low-dose anthracycline. miR-10b was overexpressed specifically in cells with high metastatic potential, suggesting a role for this miRNA as a metastasis-specific therapeutic target. Taken together, our results implied the existence of pathways that regulate the viability and proliferation of tumor cells only after they have acquired the ability to grow at distant metastatic sites. As illustrated by miR-10b targeting, such metastasis-dependent apoptotic pathways would offer attractive targets for further therapeutic exploration. Cancer Res; 75(20); 4407–15. ©2015 AACR.
Feed-Forward Reciprocal Activation of PAFR and STAT3 Regulates Epithelial–Mesenchymal Transition in Non–Small Cell Lung Cancer
Authors: Chen Jie , Lan Tian , Zhang Weimin , Dong Lijia , Kang Nan , Zhang Shumin , Fu Ming , Liu Bing , Liu Kangtai , Zhan Qimin 
Published: September 2015

Abstract
Platelet-activating factor receptor (PAFR), a G-protein–coupled receptor, has been implicated in tumorigenesis, but its contributions to metastatic progression have not been investigated. Here, we show that PAFR is overexpressed in non–small cell lung cancer (NSCLC) as well as in breast, colorectal, and gastric carcinomas. Expression of PAFR correlates closely with clinical stages, survival time, and distant metastasis. In human NSCLC cells, activation of the PAF/PAFR signaling axis accentuated malignant character, including by stimulating epithelial–mesenchymal transition (EMT). In contrast, silencing PAFR in aggressive NSCLC cells inhibited these effects. Mechanistic investigations showed that PAFR stimulated EMT by activating STAT3 via upregulation of G-protein–dependent SRC or JAK2 kinase activity. Notably, STAT3 transcriptionally elevated PAFR expression. Thus, activation of PAFR in NSCLC cells initiated a forward feedback loop responsible for mediating the aggressive malignant character of NSCLC cells in vitro and in vivo. Reinforcing this reciprocal activation loop, PAF/PAFR signaling also upregulated IL6 expression and thereby STAT3 activation. Overall, our results elucidated an important role for PAFR dysregulation in the pathogenicity of NSCLC and unraveled a forward feedback loop between PAFR and STAT3 that acts to drive the malignant progression of NSCLC. Cancer Res; 75(19); 4198–210. ©2015 AACR.
Neutralization of KIT Oncogenic Signaling in Leukemia with Antibodies Targeting KIT Membrane Proximal Domain 5
Authors: Le Gall Marianne , Crépin Ronan , Neiveyans Madeline , Auclair Christian , Fan Yongfeng , Zhou Yu , Marks James D. , Pèlegrin André , Poul Marie-Alix 
Published: September 2015

AbstractKIT is a cell surface tyrosine kinase receptor whose ligand stem cell factor (SCF) triggers homodimerization and activation of downstream effector pathways involved in cell survival, proliferation, homing, or differentiation. KIT-activating mutations are major oncogenic drivers in subsets of acute myeloid leukemia (AML), in mast cell leukemia, and in gastrointestinal stromal tumors (GIST). The overexpression of SCF and/or wild-type (WT) KIT is also observed in a number of cancers, including 50% of AML and small cell lung cancer. The use of tyrosine kinase inhibitors (TKI) in these pathologies is, however, hampered by initial or acquired resistance following treatment. Using antibody phage display, we obtained two antibodies (2D1 and 3G1) specific for the most membrane proximal extracellular immunoglobulin domain (D5) of KIT, which is implicated in KIT homodimerization. Produced as single chain variable antibody fragments fused to the Fc fragment of a human IgG1, bivalent 2D1-Fc and 3G1-Fc inhibited KIT-dependent growth of leukemic cell lines expressing WT KIT (UT7/Epo) or constitutively active KIT mutants, including the TKI imatinib-resistant KIT D816V mutant (HMC1.2 cell line). In all models, either expressing WT KIT or mutated KIT, 2D1 and 3G1-Fc induced KIT internalization and sustained surface downregulation. However, interestingly, KIT degradation was only observed in leukemic cell lines with oncogenic KIT, a property likely to limit the toxicity of these antibodies in patients. These fully human antibody formats may represent therapeutic tools to target KIT signaling in leukemia or GIST, and to bypass TKI resistance of certain KIT mutants. Mol Cancer Ther; 14(11); 2595–605. ©2015 AACR.
Impact of p53 Status on Radiosensitization of Tumor Cells by MET Inhibition–Associated Checkpoint Abrogation
Authors: Mikami K. , Medová M. , Nisa L. , Francica P. , Glück A.A. , Tschan M.P. , Blaukat A. , Bladt F. , Aebersold D.M. , Zimmer Y. 
Published: September 2015

Abstract
Signaling via the MET receptor tyrosine kinase has been implicated in crosstalk with cellular responses to DNA damage. Our group previously demonstrated that MET inhibition in tumor cells with deregulated MET activity results in radiosensitization via downregulation of the ATR–CHK1–CDC25 pathway, a major signaling cascade responsible for intra-S and G2–M cell-cycle arrest following DNA damage. Here we aimed at studying the potential therapeutic application of ionizing radiation in combination with a MET inhibitor, EMD-1214063, in p53-deficient cancer cells that harbor impaired G1–S checkpoint regulation upon DNA damage. We hypothesized that upon MET inhibition, p53-deficient cells would bypass both G1–S and G2–M checkpoints, promoting premature mitotic entry with substantial DNA lesions and cell death in a greater extent than p53-proficient cells. Our data suggest that p53-deficient cells are more susceptible to EMD-1214063 and combined treatment with irradiation than wild-type p53 lines as inferred from elevated γH2AX expression and increased cytotoxicity. Furthermore, cell-cycle distribution profiling indicates constantly lower G1 and higher G2–M population as well as higher expression of a mitotic marker p-histone H3 following the dual treatment in p53 knockdown isogenic variant, compared with the parental counterpart.
Implications: The concept of MET inhibition-mediated radiosensitization enhanced by p53 deficiency is of high clinical relevance, as p53 is frequently mutated in numerous types of human cancer. The current data point for a therapeutic advantage for an approach combining MET targeting along with DNA-damaging agents for MET-positive/p53-negative tumors. Mol Cancer Res; 13(12); 1544–53. ©2015 AACR.
A política do grupo: modos de produção como ato político
Authors: Moreira Carina Maria Guimarães 
Published: September 2015

<div class="page" title="Page 2"><div class="layoutArea"><div class="column"><p><span>O presente artigo propõe pensar a relação estreita entre os processos históricos socioeconômicos de configuração dos modos de produção da arte e seus rumos temáticos e formais. A partir da experiência da Cia. São Jorge de Variedades e de seu trabalho teatral </span><em>Barafonda</em><span>, buscaremos refletir sobre possíveis relações entre os movimentos considerados neste trabalho como políticos que se deflagraram historicamente na conformação de determinados modos de produção dos grupos teatrais paulistas nos últimos anos, ancorados pela Lei Municipal de Fomento ao Teatro e suas possíveis inscrições nas propostas estético-formais da companhia e do espetáculo. </span></p></div></div></div>
Apolipoprotein A-I Limits the Negative Effect of Tumor Necrosis Factor on Lymphangiogenesis
Authors: Bisoendial Radjesh , Tabet Fatiha , Tak Paul P. , Petrides Francine , Cuesta Torres Luisa F. , Hou Liming , Cook Adam , Barter Philip J. , Weninger Wolfgang , Rye Kerry-Anne 
Published: September 2015

Objective—
Lymphatic endothelial dysfunction underlies the pathogenesis of many chronic inflammatory disorders. The proinflammatory cytokine tumor necrosis factor (TNF) is known for its role in disrupting the function of the lymphatic vasculature. This study investigates the ability of apolipoprotein (apo) A-I, the principal apolipoprotein of high-density lipoproteins, to preserve the normal function of lymphatic endothelial cells treated with TNF.

Approach and Results—
TNF decreased the ability of lymphatic endothelial cells to form tube-like structures. Preincubation of lymphatic endothelial cells with apoA-I attenuated the TNF-mediated inhibition of tube formation in a concentration-dependent manner. In addition, apoA-I reversed the TNF-mediated suppression of lymphatic endothelial cell migration and lymphatic outgrowth in thoracic duct rings. ApoA-I also abrogated the negative effect of TNF on lymphatic neovascularization in an ATP-binding cassette transporter A1-dependent manner. At the molecular level, this involved downregulation of TNF receptor-1 and the conservation of prospero-related homeobox gene-1 expression, a master regulator of lymphangiogenesis. ApoA-I also re-established the normal phenotype of the lymphatic network in the diaphragms of human TNF transgenic mice.

Conclusions—
ApoA-I restores the neovascularization capacity of the lymphatic system during TNF-mediated inflammation. This study provides a proof-of-concept that high-density lipoprotein–based therapeutic strategies may attenuate chronic inflammation via its action on lymphatic vasculature.
Trends in Stroke Incidence and 28-Day Case Fatality in a Nationwide Stroke Registry of a Multiethnic Asian Population
Authors: Tan Chuen Seng , Müller-Riemenschneider Falk , Ng Sheryl Hui Xian , Tan Pei Zheng , Chan Bernard P.L. , Tang Kok-Foo , Ahmad Aftab , Kong Keng He , Chang Hui Meng , Chow Khuan Yew , Koh Gerald Choon-Huat , Venketasubramanian Narayanaswamy 
Published: September 2015

Background and Purpose—
This study investigated trends in stroke incidence and case fatality overall and according to sex, age, ethnicity, and stroke subtype in a multiethnic Asian population.

Methods—

The Singapore Stroke Registry identifies all stroke cases in all public hospitals using medical claims, hospital discharge summaries, and death registry data. Age-standardized incidence rates and 28-day case-fatality rates were calculated for individuals aged ≥15 years between 2006 and 2012. To estimate the annual percentage change of the rates, a linear regression model was fitted to the log rates, and a Wald test was performed to test for trend.
P
values <0.05 were considered significant.

Results—
A total of 40 623 cases were recorded. The total stroke incidence fell by ≈12.0%, and case fatality fell by 17.2% in the study. Declining trends in stroke incidence were stronger in women (female: −2.94; 95% confidence interval [CI], −3.43 to −2.44; male: −1.80; 95% CI, −2.58 to −1.02); in the older age groups (≥65 years: −3.62; 95% CI, −4.30 to −2.94; 50–64 years: −1.26; 95% CI, −1.97 to −0.55; <50 years: 3.33; 95% CI, 1.49 to 5.20), in Chinese (−2.64; 95% CI, −3.15 to −2.13), Indians (−3.78; 95% CI, −5.93 to −1.58), and others (−12.73; 95% CI, −18.93 to −6.06) compared with Malays (2.58; 95% CI, 1.17 to 4.02); and in ischemic stroke subtype (ischemic: −2.43; 95% CI, −3.13 to −1.73; hemorrhagic: −1.02; 95% CI, −2.04 to 0.01). Subgroup-specific findings for case fatality were similar.

Conclusion—
This is the first countrywide hospital-based registry study in a multiethnic Asian population, and it revealed marked overall reductions in stroke incidence and case fatality. However, it also identified important population groups with less favorable trends, especially younger adults and those of Malay ethnicity.
“I am the Buddha, the Buddha is Me”: Concentration Meditation and Esoteric Modern Buddhism in Burma/Myanmar
Authors: Foxeus Niklas 
Published: September 2015

In postcolonial Burma, two trends within lay Buddhism — largely in tension with one another — developed into large-scale movements. They focused upon different meditation practices, insight meditation and concentration meditation, with the latter also including esoteric lore. An impetus largely shared by the movements was to define an “authentic” Buddhism to serve as the primary vehicle of the quest for individual, local, and national identity. While insight meditation was generally considered Buddhist meditationpar excellence, concentration meditation was ascribed a more dubious Buddhist identity. Given this ambiguity, it could be considered rather paradoxical that concentration meditation could be viewed as a source of “authentic” Buddhism.The aim of this article is to investigate the issue of identity and the paradox of authenticity by examining the concentration meditation practices of one large esoteric congregation and tentatively comparing its practices with those of the insight meditation movement. It will be argued that the movements represented two varieties of so-called modern Buddhism (rationalist modern Buddhism and esoteric modern Buddhism) drawing on different Buddhist imaginaries and representing two main trends that are largely diametrically opposed to one another. They therefore represent two ways of constructing an individual, local, and national identity.
Nematicidal activities of bacterial volatiles and components from two marine bacteria, Pseudoalteromonas marina strain H-42 and Vibrio atlanticus strain S-16, against the pine wood nematode, Bursaphelenchus xylophilus
Authors: Yu Jie , Du Guicai , Li Ronggui , Li Li , Li Zi , Zhou Changjing , Chen Congcong , Guo Daosen 
Published: September 2015

Bacterial volatiles represent an important source for new natural nematicidal compounds that may be developed as novel nematicidal agents to control plant-parasitic nematodes. In this study, two marine bacteria,Pseudoalteromonas marinastrain H-42 andVibrio atlanticusstrain S-16, were isolated from seawater and the bay scallop (Argopecten irradians), respectively, collected from the subtidal beds at Qingdao, China.In vitrobioassays indicated that culture supernatants of the two bacteria displayed strong nematicidal activities with corrected mortalities of more than 93% after 24 h exposure against the pine wood nematode (PWN),Bursaphelenchus xylophilus. Maximal nematicidal activity was exhibited by culture supernatants of the two marine bacteria incubated at 15-20°C for 3 days in Zobell 2216E medium prepared with 100% seawater and initial pH 6.0-7.0, inoculating 19-h-old cultures. Through analysis using solid-phase microextraction-gas chromatography/mass spectrometry, the volatiles produced by the two bacteria were shown to contain mainly dimethyl disulphide, benzaldehyde, dimethyl trisulphide,tert-butylamine, acetone and dimethylamine, N-(diisopropylphosphino)methyl-. Results from nematicidal assay using pure commercial compounds instead of the candidate volatiles demonstrated that the four volatile compounds, dimethyl trisulphide (LC90 = 0.060 mmol l−1), benzaldehyde (LC90 = 0.309 mmol l−1), dimethyl disulphide (LC90 = 0.162 mmol l−1) andtert-butylamine (LC90 = 0.366 mmol l−1), showed strong nematicidal activities against PWN. This is the first report on the nematicidal activity of volatile organic compounds (VOC) from marine microorganisms. This study indicates that the nematicidal VOC produced by marine bacteria are potential substitutes for current chemical control options of pine wilt disease caused by PWN which greatly threatens global forest resources.