bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biological and Clinical Availability of Adipose-Derived Stem Cells for Pelvic Dead Space Repair
Authors: Takahashi Hidekazu , Haraguchi Naotsugu , Nishikawa Shimpei , Miyazaki Susumu , Suzuki Yozou , Mizushima Tsunekazu , Nishimura Junichi , Takemasa Ichirou , Yamamoto Hirofumi , Mimori Koshi , Ishii Hideshi , Doki Yuichiro , Mori Masaki 
Published: October 2012

Abstract
Adipose-derived stem cells (ADSCs) are a very attractive cell source for regenerative and reconstructive medicine. Although ADSCs have already been used in cardiovascular disease and cosmetic surgery, they have not yet been used in gastroenterological surgery. In this study, we clarified the utility of the combined application of ADSCs and resected intraperitoneal fatty tissues as a sealant for the pelvic dead space that sometimes causes severe and fatal complications in colorectal and gynecological surgeries. In pelvic dead space model mice, mouse ADSCs efficiently maintained transplanted intraperitoneal fatty tissues without any incidence of adhesion to surrounding organs. In vivo and in vitro analyses revealed that transplanted ADSCs differentiated into endothelial cells by expressing the angiogenic factors vascular endothelial growth factor and hepatocyte growth factor. Mouse and human ADSCs contained a CD45−CD34+ subset possessing high colony formation and sphere formation abilities. In addition, the CD45−CD34+ subset consisted of two characteristic subsets: the CD34+CD90+ angiogenic subset and the CD34+CD90− adipogenic subset. Grafts of human ADSCs with fat transplanted into mice were efficiently maintained for more than 12 months without volume reductions. A comparative study of graft maintenance efficacy between cultured human ADSCs and freshly isolated ADSCs indicated that the cultivation of ADSCs decreased their graft maintenance ability. These findings suggested that the angiogenic and adipogenic subsets act in coordination with each other and are essential for efficient graft maintenance.
Constructivism in Teaching Elementary Algebra: It’s Influence in Mathematics Performance of Students in Large Classes
Authors: Lopez Edmundo Cabiltes 
Published: October 2012

This study determined the effect of constructivist method in students’ mathematics achievement. It sought to answer the influence of teaching methods in students’ achievement scores in large classes in terms of knowledge, comprehension, application, and analysis questions. A pretest- posttest quasi-experimental control group design was used. The experimental group was exposed to constructivist method while the control group to traditional method. To determine the influence of the methods in the students’ achievement scores, the one way ANCOVA was used. The researcher handled the two classes. Results showed that there was a significant difference in the students’ achievement scores in terms of knowledge, comprehension; application and analysis questions. The control group performed better than the experimental group in all levels of questions. Constructivist approach is ineffective in large classes while the lecture method is an appropriate teaching approach for large classes. Keywords - constructivism, large classes, mathematics achievement, experimental
Adherence to the Use of ICT for Classroom Instruction: Its Impact on Students’ Learning
Authors: Gomez Rizalina G. 
Published: October 2012

This study investigated the teachers’ adherence to the use of ICT for classroom instruction and its impact on the students’ learning. One hundred twenty-five (125) student teachers from the College of Education accomplished the questionnaires. Student teachers were found to have strong adherence to the use of ICT in teaching. This analysis elucidated teacher educators’ access to ICT, their intensity of use, their training skills, and their confidence in using ICT. Pedagogical practices included the use of web-enhanced learning activities and a combination of Web 2.0 tools in teaching. Exploratory multiple regression analysis addressed the importance of these different factors at the level of the educator in the use of ICT in teaching practice. Results showed students’ academic performance generally improved with increased teachers’ training skills. The intensive use of ICT as a tool in teaching had posed a significant impact on the students’ learning. It was therefore recommended for teacher educators to sustain the use of ICT as an effective strategy and continue to access the skills-based approach in the integration of ICT in education. Keywords - Academic Performance, ICT, Web 2.0 tools, Web- Enhanced Learning Activities, Pre-service Teachers, Pedagogical Practices
Prime‐, Stress‐, and Cue‐Induced Reinstatement of Extinguished Drug‐Reinforced Responding in Rats: Cocaine as the Prototypical Drug of Abuse
Authors: Beardsley Patrick M. , Shelton Keith L. 
Published: October 2012

AbstractThis unit describes the testing of rats in prime‐, footshock‐, and cue‐induced reinstatement procedures. Evaluating rats in these procedures enables the assessment of treatments on behavior thought to model drug relapse precipitated by re‐contact with an abused drug (prime‐induced), induced by stress (footshock‐induced), or by stimuli previously associated with drug administration (cue‐induced). For instance, levels of reinstatement under the effects of test compound administration could be compared to levels under vehicle administration to help identify potential treatments for drug relapse, or reinstatement levels of different rat strains could be compared to identify potential genetic determinants of perseverative drug‐seeking behavior. Cocaine is used as a prototypical drug of abuse, and relapse to its use serves as the model in this unit, but other self‐administered drugs could readily be substituted with little modification to the procedures. Curr. Protoc. Neurosci. 61:9.39.1‐9.39.40. © 2012 by John Wiley & Sons, Inc.
Electrospun Fibers as a Solid‐State Real‐Time Zinc Ion Sensor with High Sensitivity and Cell Medium Compatibility
Authors: Syu Jia‐Hao , Cheng Yi‐Kai , Hong Wun‐Yan , Wang Hsing‐Ping , Lin Yu‐Chao , Meng Hsin‐Fei , Zan Hsiao‐Wen , Horng Sheng‐Fu , Chang Gao‐Fong , Hung Chen‐Hsiung , Chiu Yu‐Cheng , Chen Wen‐Chang , Tsai May‐Jywan , Cheng Henrich 
Published: October 2012

Abstractmeso‐2,6‐Dichlorophenyltripyrrinone (TPN‐Cl2), a probe molecule for zinc II ions, is dispersed in a polymer host. The red fluorescence peak at 620 nm appears when the molecule forms a complex with zinc at its center. TPN‐Cl2 has a high selectivity for zinc II and tolerates many common metal ions present in the human body. The probe molecules are blended with a hydrogel polymer, poly(2‐hydroxyethyl methacrylate) (poly HEMA), with 30 wt% dimethylformamide (DMF). The fiber structure with 1 μm diameter is made by electrospinning in DMF solution of the probe and poly HEMA mixture. The fibrous film detects zinc ions with concentrations as low as 10−6 M in real‐time both in water and in the commonly used cell culture liquid media Dulbecco's modified Eagle medium (DMEM) and fetal bovine serum (FBS), which contain many metal ions and proteins. The time‐resolution is 5 min for 10−6 M and 1 min for 10−5 M. This sensitivity and response speed satisfy the requirements for non‐invasive biomedical studies.
Nanofiber adsorbents for high productivity downstream processing
Authors: Hardick Oliver , Dods Stewart , Stevens Bob , Bracewell Daniel G. 
Published: October 2012

AbstractElectrospun polymeric nanofiber adsorbents offer an alternative ligand support surface for bioseparations. Their non‐woven fiber structure with diameters in the sub‐micron range creates a remarkably high surface area. To improve the purification productivity of biological molecules by chromatography, cellulose nanofiber adsorbents were fabricated and assembled into a cartridge and filter holder format with a volume of 0.15 mL, a bed height of 0.3 mm and diameter of 25 mm. The present study investigated the performance of diethylaminoethyl (DEAE) derivatized regenerated cellulose nanofiber adsorbents based on criteria including mass transfer and flow properties, binding capacity, and fouling effects. Our results show that nanofibers offer higher flow and mass transfer properties. The non‐optimized DEAE‐nanofiber adsorbents indicate a binding capacity of 10% that of packed bed systems with BSA as a single component system. However, they operate reproducibly at flowrates of a hundred times that of packed beds, resulting in a potential productivity increase of 10‐fold. Lifetime studies showed that this novel adsorbent material operated reproducibly with complex feed material (centrifuged and 0.45 µm filtered yeast homogenate) and harsh cleaning‐in‐place conditions over multiple cycles. DEAE nanofibers showed superior operating performance in permeability and fouling over conventional adsorbents indicating their potential for bioseparation applications. Biotechnol. Bioeng. 2013; 110: 1119–1128. © 2012 Wiley Periodicals, Inc.
Enhanced nitrogen removal in single‐chamber microbial fuel cells with increased gas diffusion areas
Authors: Yan Hengjing , Regan John M. 
Published: October 2012

AbstractSingle‐chamber microbial fuel cells (MFCs) with nitrifiers pre‐enriched at the air cathodes have previously been demonstrated as a passive strategy for integrating nitrogen removal into current‐generating bioelectrochemical systems. To further define system design parameters for this strategy, we investigated in this study the effects of oxygen diffusion area and COD/N ratio in continuous‐flow reactors. Doubling the gas diffusion area by adding an additional air cathode or a diffusion cloth significantly increased the ammonia and COD removal rates (by up to 115% and 39%), ammonia removal efficiency (by up to 134%), the cell voltage and cathode potentials, and the power densities (by a factor of approximately 2). When the COD/N ratio was lowered from 13 to 3, we found up to 244% higher ammonia removal rate but at least 19% lower ammonia removal efficiency. An increase of COD removal rate by up to 27% was also found when the COD/N ratio was lowered from 11 to 3. The Coulombic efficiency was not affected by the additional air cathode, but decreased by an average of 11% with the addition of a diffusion cloth. Ammonia removal by assimilation was also estimated to understand the ammonia removal mechanism in these systems. These results showed that the doubling of gas diffusion area enhanced N and COD removal rates without compromising electrochemical performance. Biotechnol. Bioeng. 2013; 110: 785–791. © 2012 Wiley Periodicals, Inc.
Zonal rate model for axial and radial flow membrane chromatography. Part I: Knowledge transfer across operating conditions and scales
Authors: Ghosh Pranay , Vahedipour Kaveh , Lin Min , Vogel Jens H. , Haynes Charles A. , von Lieres Eric 
Published: October 2012

AbstractThe zonal rate model (ZRM) has previously been applied for analyzing the performance of axial flow membrane chromatography capsules by independently determining the impacts of flow and binding related non‐idealities on measured breakthrough curves. In the present study, the ZRM is extended to radial flow configurations, which are commonly used at larger scales. The axial flow XT5 capsule and the radial flow XT140 capsule from Pall are rigorously analyzed under binding and non‐binding conditions with bovine serum albumin (BSA) as test molecule. The binding data of this molecule is much better reproduced by the spreading model, which hypothesizes different binding orientations, than by the well‐known Langmuir model. Moreover, a revised cleaning protocol with NaCl instead of NaOH and minimizing the storage time has been identified as most critical for quantitatively reproducing the measured breakthrough curves. The internal geometry of both capsules is visualized by magnetic resonance imaging (MRI). The flow in the external hold‐up volumes of the XT140 capsule was found to be more homogeneous as in the previously studied XT5 capsule. An attempt for model‐based scale‐up was apparently impeded by irregular pleat structures in the used XT140 capsule, which might lead to local variations in the linear velocity through the membrane stack. However, the presented approach is universal and can be applied to different capsules. The ZRM is shown to potentially help save valuable material and time, as the experiments required for model calibration are much cheaper than the predicted large‐scale experiment at binding conditions. Biotechnol. Bioeng. 2013; 110: 1129–1141. © 2012 Wiley Periodicals, Inc.
Corporate Environmental and Economic Performance of Japanese Manufacturing Firms: Empirical Study for Sustainable Development
Authors: Fujii Hidemichi , Iwata Kazuyuki , Kaneko Shinji , Managi Shunsuke 
Published: October 2012

ABSTRACTThis study examines the relationship between environmental performance and economic performance in Japanese manufacturing firms. The environmental performance indicators include CO2 emissions and the aggregate toxic risk associated with chemical emissions relative to sales. Return on assets (ROA) is used as an indicator of economic performance. We demonstrate that there is a significant inverted U‐shaped relationship between ROA and environmental performance calculated by aggregated toxic risk. We also find that the environmental performance increases ROA through both returns on sales and improved capital turnover. However, we observe a significant positive relationship between financial performance and environmental performance based on CO2 emissions. These findings may provide evidence for the consequences of firms' environmental behavior and sustainable development. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment.
Sustainable Development in the Transport Sector: Influencing Environmental Behaviour and Performance
Authors: Oberhofer Peter , Fürst Elmar 
Published: October 2012

ABSTRACTSustainable Development and Environmental Policy are becoming increasingly important in the development of business strategies. Consequently, corporate road freight transport comes under particular scrutiny due to its substantial impact on the environment. This paper explores the implementation of environmental management in the Austrian road freight transport sector. A model based on an extensive literature review is developed and adapted to show the impact of attitude, company size and sector affiliation. It is tested using data from a quantitative survey. Based on an extensive literature review a model of influencing factors is developed and tested using data from a quantitative survey. According to our findings, we adopted the model with respect to the impact of attitude, company size and sector affiliation. Although decision‐makers' attitudes have a significant influence on the companies' actual environmental performance, firm size and sector affiliation are shown to have a far greater influence. It is therefore necessary to offer external incentives and support in order to improve environmental behaviour, especially among smaller and transport companies. Such instruments, however, need to be aligned on an international basis and across industries to prevent friction and competitive disadvantage. In addition to policy intervention (legal restrictions and incentives) and improvements on a corporate level, consumer awareness should be raised in order to create public pressure for companies to implement environmental management. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment.
Lithium Choreography: Intramolecular Arylations of Carbamate‐Stabilised Carbanions and Their Mechanisms Probed by In Situ IR Spectroscopy and DFT Calculations
Authors: Fournier Anne M. , Nichols Christopher J. , Vincent Mark A. , Hillier Ian H. , Clayden Jonathan 
Published: October 2012

AbstractDeprotonation of O‐allyl, O‐propargyl or O‐benzyl carbamates in the presence of a lithium counterion leads to carbamate‐stabilised organolithium compounds that may be quenched with electrophiles. We now report that when the allylic, propargylic or benzylic carbamate bears an N‐aryl substituent, an aryl migration takes place, leading to stereochemical inversion and C‐arylation of the carbamate α to oxygen. The aryl migration is an intramolecular SNAr reaction, despite the lack of anion‐stabilising aryl substituents. Our in situ IR studies reveal a number of intermediates along the rearrangement pathway, including a “pre‐lithiation complex,” the deprotonated carbamate, the rearranged anion, and the final arylated carbamate. No evidence was obtained for a dearomatised intermediate during the aryl migration. DFT calculations predict that during the reaction the solvated Li cation moves from the carbanion centre, thus freeing its lone pair for nucleophilic attack on the remote phenyl ring. This charge separation leads to several alternative conformations. The one having Li+ bound to the carbamate oxygen gives rise to the lowest‐energy transition structure, and also leads to inversion of the configuration. In agreement with the IR studies, the DFT calculations fail to locate a dearomatised intermediate.
Supramolecular Polymer Hydrogels from Bolaamphiphilic L‐Histidine and Benzene Dicarboxylic Acids: Thixotropy and Significant Enhancement of EuIII Fluorescence
Authors: Liu Yaqing , Wang Tianyu , Liu Minghua 
Published: October 2012

AbstractSupramolecular polymers from the bolaamphiphilic L‐histidine (BolaHis) and benzene dicarboxylic acids (o‐phthalic acid, OPA; isophthalic acid, IPA and terephthalic acid, TPA) were found to form hydrogels although neither of the single components could gel water. It was suggested that the hydrogen bond and ionic interactions among different imidazole and carboxylic acid groups are responsible for the formation of the supramolecular polymer as well as the hydrogel formation. Depending on the structures of the dicarboxylic acids, different behaviors of the gels were observed. The hydrogels from OPA/BolaHis and IPA/BolaHis showed thixotropic properties, that is, the hydrogel was destroyed by hand shaking and then slowly gelated again at room temperature. However, the hydrogels of TPA/BolaHis could not. Interestingly, when EuIII was doped into IPA/BolaHis supramolecular polymers, very strong luminescence enhancement was observed. FT‐IR spectroscopies and XRD analysis revealed that the strong luminescence enhancement could be attributed to the matched supramolecular nanostructures, which render the correct binding and a good dispersion of EuIII ions. The work offers a new approach for fabricating functional hydrogels through the supramolecular polymers.
Density Functional Theory Investigation on the Second‐Order Nonlinear Optical Properties of Chlorobenzyl‐o‐Carborane Derivatives
Authors: Liu Yan , Yang Guochun , Sun Shiling , Su Zhongmin 
Published: October 2012

AbstractThe structures and second‐order nonlinear optical (NLO) properties of a series of chlorobenzyl‐o‐carboranes derivatives (1–12) containing different push‐pull groups have been studied by density functional theory (DFT) calculation. Our theoretical calculations show that the static first hyperpolarizability (βtot) values gradually increase with increasing the π‐conjugation length and the strength of electron donor group. Especially, compound 12 exhibits the largest βtot (62.404×10−30 esu) by introducing tetrathiafulvalene (TTF), which is about 76 times larger than that of compound 1 containing aryl. This means that the appropriate structural modification can substantially increase the first hyperpolarizabilities of the studied compounds. For the sake of understanding the origin of these large NLO responses, the frontier molecular orbitals (FMOs), electron density difference maps (EDDMs), orbital energy and electronic transition energy of the studied compounds are analyzed. According to the two‐state model, the lower transition energy plays an important role in increasing the first hyperpolarizability values. This study may evoke possible ways to design preferable NLO materials.
A Convenient Hydrogenation Method for the Synthesis of Metallo‐mesoporphyrin IX Dimethyl Esters via Self‐catalyzed CoCl2‐NaBH4 Reagent System
Authors: Xu Shichao , Hu Bingcheng , Hu Tianjing , Wang Huan , Huang Xiuyou , Lou Xingkun , Liu Zuliang 
Published: October 2012

AbstractA convenient protocol has been developed for the hydrogenation of metallo‐protoporphyrin IX dimethyl esters (MPPDMEs) to their mesoporphyrin analogues using CoCl2‐NaBH4 reagent system. Metallo‐porphyrin complexes were found to perform as self‐catalysts in this procedure. This method provides several advantages such as safe and simple procedure, short reaction time, high yields and low cost.
Synthesis of 2(5H)‐Furanone Derivatives with Bis‐1,2,3‐triazole Structure
Authors: Huo Jingpei , LÜ Meixiang , Wang Zhaoyang , Li Yizhong 
Published: October 2012

AbstractA series of new chiral 2(5H)‐furanone derivatives containing bis‐1,2,3‐triazole moiety were designed and synthesized from (5S)‐5‐alkoxy‐3,4‐dihalo‐2(5H)‐furanones 1, dicarboxyl amino acids 2, propargyl bromide, and organic azides 5 under mild conditions via the sequential three steps, including asymmetric Michael addition‐elimination, substitution and no‐ligand click reaction. Twelve new intermediates, including N‐[5‐alkoxy‐2(5H)‐furanonyl] dicarboxyl amino acids 3 and their corresponding propargyl esters 4, and twelve target molecules 6 were characterized by FTIR, 1H NMR, 13C NMR, MS and elemental analysis. The influences of different synthetic conditions and substrates in each step were investigated. The research provides a new method and idea for the synthesis of 2(5H)‐furanone compounds with polyheterocyclic structure due to the diversities of four basic unit molecules.
Theoretical Investigation on the Electron and Energy Transfer between Peripheral Carrier Transport Groups and Central Chromophores in Electroluminescent Materials
Authors: Pan Yuyu , Liu Dandan , Xu Hai , Liu Xiaodong , Sun Guannan , Yang Bing , Ma Yuguang 
Published: October 2012

AbstractThe molecular materials with structures of luminescent core and peripheral carrier groups (e.g. carbazoles), have exhibited high‐performance in organic light‐emitting diodes (OLEDs). Present work is to understand the basic process of electronic and energy exchange between the peripheral functional groups and the central core through quantum chemical analysis. As an example, 4,7‐bis(9,9‐bis(6‐(9H‐carbazol‐9‐yl)hexyl)‐9H‐fluoren‐2‐yl)benzo[c]‐[1,2,5]thiadiazole (TCBzC) is investigated in regards to optoelectronic properties using density functional theory (DFT). The results suggest that the forbidden transition from peripheral carbazole to the central chromophore core makes for separated electrical and optical properties, and high performance electroluminescence (EL) is mainly attributed to the energy‐transfer from carbazoles to the fluorene derivative core