bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global SUMOylation on active chromatin is an acute heat stress response restricting transcription
Authors: Niskanen Einari A. , Malinen Marjo , Sutinen Päivi , Toropainen Sari , Paakinaho Ville , Vihervaara Anniina , Joutsen Jenny , Kaikkonen Minna U. , Sistonen Lea , Palvimo Jorma J. 
Published: July 2015

Abstract
Background
Cells have developed many ways to cope with external stress. One distinctive feature in acute proteotoxic stresses, such as heat shock (HS), is rapid post-translational modification of proteins by SUMOs (small ubiquitin-like modifier proteins; SUMOylation). While many of the SUMO targets are chromatin proteins, there is scarce information on chromatin binding of SUMOylated proteins in HS and the role of chromatin SUMOylation in the regulation of transcription.

Results
We mapped HS-induced genome-wide changes in chromatin occupancy of SUMO-2/3-modified proteins in K562 and VCaP cells using ChIP-seq. Chromatin SUMOylation was further correlated with HS-induced global changes in transcription using GRO-seq and RNA polymerase II (Pol2) ChIP-seq along with ENCODE data for K562 cells. HS induced a rapid and massive rearrangement of chromatin SUMOylation pattern: SUMOylation was gained at active promoters and enhancers associated with multiple transcription factors, including heat shock factor 1. Concomitant loss of SUMOylation occurred at inactive intergenic chromatin regions that were associated with CTCF-cohesin complex and SETDB1 methyltransferase complex. In addition, HS triggered a dynamic chromatin binding of SUMO ligase PIAS1, especially onto promoters. The HS-induced SUMOylation on chromatin was most notable at promoters of transcribed genes where it positively correlated with active transcription and Pol2 promoter-proximal pausing. Furthermore, silencing of SUMOylation machinery either by depletion of UBC9 or PIAS1 enhanced expression of HS-induced genes.

Conclusions
HS-triggered SUMOylation targets promoters and enhancers of actively transcribed genes where it restricts the transcriptional activity of the HS-induced genes. PIAS1-mediated promoter SUMOylation is likely to regulate Pol2-associated factors in HS.
Early medication use in new-onset rheumatoid arthritis may delay joint replacement: results of a large population-based study
Authors: Moura Cristiano S. , Abrahamowicz Michal , Beauchamp Marie-Eve , Lacaille Diane , Wang Yishu , Boire Gilles , Fortin Paul R. , Bessette Louis , Bombardier Claire , Widdifield Jessica , Hanly John G. , Feldman Debbie , Maksymowych Walter , Peschken Christine , Barnabe Cheryl , Edworthy Steve , Bernatsky Sasha ,   CAN-AIM
Published: July 2015

Abstract
Introduction
Use of disease-modifying anti-rheumatic drugs (DMARDs) in rheumatoid arthritis (RA) may prevent joint damage and potentially reduce joint replacement surgeries. We assessed the association between RA drug use and joint replacement in Quebec, Canada.

Methods
A cohort of new-onset RA patients was identified from Quebec’s physician billing and hospitalization databases from 2002–2011. The outcome was defined using procedure codes submitted by orthopedic surgeons. Medication use was obtained from pharmacy databases. We used alternative Cox regression models with time-dependent variables measuring the cumulative effects of past use during different time windows (one model focussing on the first year after cohort entry) for methotrexate (MTX), and other DMARDs. Models were adjusted for baseline sociodemographics, co-morbidity and prior health service use, time-dependent cumulative use of other drugs (anti-tumor necrosis factor [anti-TNF] agents, other biologics, cyclooxygenase-2 inhibitors [COXIBs], nonselective nonsteroidal antiinflammatory drugs [NSAIDs], and systemic steroids), and markers of disease severity.

Results
During follow-up, 608 joint replacements occurred among 11,333 patients (median follow-up: 4.6 years). The best-fitting model relied on the cumulative early use (within the first year after cohort entry) of MTX and of other DMARDs, with an interaction between MTX and other DMARDs. In this model, greater exposure within the first year, to either MTX (adjusted hazard ratio, HR = 0.95 per 1 month, 95 % confidence interval, 95 % CI 0.93-0.97) or other DMARDs (HR = 0.97, 95 % CI 0.95-0.99) was associated with longer time to joint replacement.

Conclusions
Our results suggest that longer exposure to either methotrexate (MTX) or other DMARDs within the first year after RA diagnosis is associated with longer time to joint replacement surgery.
Emergence of spatial behavioral function and associated mossy fiber connectivity and c-Fos labeling patterns in the hippocampus of rats
Authors: Comba Rachel , Gervais Nicole , Mumby Dave , Holahan Matthew 
Published: July 2015

Improvement on spatial tasks is observed during a late, postnatal developmental period (PND18 – PND24). The purpose of the current work was 1) to determine whether the emergence of spatial-behavioral function was based on the ability to generate appropriate behavioral output; 2) to assess whether mossy fiber connectivity patterns preceded the emergence of spatial-behavioral function; 3) to explore functional changes in the hippocampus to determine whether activity in hippocampal networks occurred in a training-dependent or developmentally-dependent fashion. To these ends, male, Long Evans rats were trained on a spatial water or dry maze task for one day (PND16, PND18 or PND20) then euthanized. Training on these 2 tasks with opposing behavioral demands (swimming versus exploration) was hypothesized to control for behavioral topology. Only at PND20 was there evidence of spatial-behavioral function for both tasks. Examination of synaptophysin staining in the CA3 region (i.e., mossy fiber projections) revealed enhanced connectivity patterns that preceded the emergence of spatial behavior. Analysis of c-Fos labeling (functional changes) revealed developmentally-dependent increases in c-Fos positive cells in the dentate gyrus, CA3 and CA1 regions whereas training-dependent increases were noted in the CA3 and CA1 regions for the water-maze trained groups. Results suggest that changes in mossy fiber connectivity in association with enhanced hippocampal functioning precede the emergence of spatial behavior observed at PND20. The combination of neuroanatomical and behavioural results confirms the hypothesis that this time represents a sensitive period for hippocampal development and modification and the emergence of spatial/ cognitive function.
Characterisation of human prostate epithelial progenitor differentiation in response to androgens
Authors: Heer R 
Published: July 2015

INTRODUCTION A stem cell model of prostate cancer tumourigenesis explains progression to castration resistant prostate cancer (CRPC) and offers novel perspectives in targeting this cancer in its more advanced forms. Androgen receptor (AR) regulated pathways are central mechanisms in progression to CRPC. However, AR was thought to be lacking in prostate stem cell enriched fractions. Potential low levels of AR expression in stem cell enriched cells were investigated and potential direct effects of androgen were examined. METHODS Human prostate stem cell enriched populations, based on high α2β1 integrin expression (α2β1hi), were selected from primary human prostate tissue in men undergoing transurethral prostatectomy or cystoprostatectomy. Effects on differentiation were assayed with flow cytometry using differentiation-specific markers. RESULTS Low levels of AR were demonstrable in α2β1hi cells following inhibition of the proteasome using MG132. Furthermore, a direct effect of androgen was shown in stabilising/inducing AR expression. Androgen treatment of α2β1hi cells was associated with the induction of differentiation using a number of differentiation-specific markers (prostatic acid phosphatase, cytokeratin 18 and AR) with increases ranging from 49% to 67% (p<0.05). These effects were blocked with the AR-specific inhibitor bicalutamide (p<0.05). These data support a role of direct androgen activity on stem cell enriched cells in the prostate and the implications of these findings are discussed.
Early and Long-Term Outcomes of Patients Undergoing Liver Resection and Diaphragm Excision for Advanced Colorectal Liver Metastases
Authors: Lordan Jeffrey T , Riga Angela , Worthington Tim R , Karanjia Nariman D 
Published: July 2015

INTRODUCTION At present, liver resection offers the best long-term outcome and only chance for cure in patients with colorectal liver metastases. However, there are no large series that report the early and long-term outcomes of patients who require simultaneous diaphragm excision. This study was designed to investigate these patients. PATIENTS AND METHODS A total of 285 consecutive liver resections were performed over a 10-year period. Of these, 258 had liver resections alone and 27 underwent liver resection and simultaneous diaphragm excision. Data were collected prospectively and analysed retrospectively. Pre-operative assessment was standardised. The outcomes between the two groups were compared. RESULT There was no difference in age, hospital stay or intra-operative blood loss. The diaphragm was histologically involved in four out of 27 resections. As a result, the cancer involved resection margin incidence was greater in the liver resection and diaphragm excision group (14.8% versus 3.9%; P = 0.12). The median tumour size was also different between the two groups (60 mm versus 30 mm; P = 0.001). The liver and diaphragm resection group had a greater peri-operative complication rate (44.4% versus 21.3%; P = 0.02) and mortality (7.4% versus 1.6%; P = 0.25). Overall and disease-free survival was significantly worse in the group who underwent simultaneous diaphragm excision and liver resection (P = 0.04 and P = 0.005, respectively). Diaphragm invasion was found to be an independent predictor of poor overall outcome (P = 0.02). CONCLUSION Liver resection and simultaneous diaphragm excision have a greater incidence of peri-operative morbidity and mortality and a significantly worse long-term outcome compared with liver resection alone. However, these data suggest that liver resection in the presence of diaphragm invasion may still offer a favourable outcome compared with chemotherapy treatment alone. Therefore, we believe that diaphragm involvement by tumour should not be a contra-indication to hepatectomy.
Management of fracture-dislocation of the lower cervical spine with the cervical pedicle screw system
Authors: Zhou Feng , Zou Jun , Gan Minfeng , Zhu Ruofu , Yang Huilin 
Published: July 2015

INTRODUCTION Usually, cervical pedicle screw fixation has been considered too risky for neurovascular structures. The purpose of this study was to investigate the method and efficacy of the cervical pedicle screw system for fracture-dislocation of the cervical spine because of its rigid fixation. PATIENTS AND METHODS A prospective study was conducted involving 48 patients with cervical spine fracture-dislocation who underwent cervical pedicle screw fixation surgery between January 2003 and January 2007. All patients had various degrees of cord injury, and they were classified according to the American Spinal Cord Injury Association (ASIA) Impairment Scale: 18 cases were grade A, 15 grade B, 10 grade C, and 5 grade D. RESULTS Six months after the operation, all patients had achieved solid bony fusion and stable fixation of the related segments. Thirty patients with incomplete spinal cord injury improved their ASIA Impairment Scale classification by 1 to 2 grades after the operation. Eighteen patients with complete spinal cord injury had no improvement in neural function. However, nerve root symptoms such as pain and numbness were alleviated to some extent. CONCLUSIONS The cervical pedicle screw system is an effective and reliable method for the restoration of cervical stability. Sufficient pre-operative imaging studies of the pedicles and strict screw insertion technique should be emphasised.
How accurately does a simulation glove reflect function compared to rheumatoid arthritis sufferers?
Authors: Hall TC , Nixon MF , Dias JJ , Graham T , Cook S 
Published: July 2015

INTRODUCTION This study assessed the ability of gloves to simulate rheumatoid arthritis of the hand. SUBJECTS AND METHODS Assessments were made in the dominant hand of 24 healthy volunteers with no glove, glove A (simulating stiffness only) and glove B (simulating stiffness and pain). Results were compared to data held on 23 rheumatoid arthritis patients. Sollerman score was used as a standardised measure of hand function and time taken to complete testing was recorded. Grip strength was also measured in volunteers. RESULTS Both gloves simulate a reduction in power and prolong time taken to complete Sollerman hand-function testing. The gloves are less able to simulate a matched reduction in function when compared to rheumatoid arthritis sufferers. Sollerman score is 9.7% less in rheumatoid arthritis hands than a healthy volunteer using the glove. CONCLUSIONS The glove could, therefore, be used to guide future design of tools and aides that accommodate for hand disorders. More work on the usefulness of such disease simulation in the design of tools for such patients is needed.
A meta-analysis of fast track surgery for patients with gastric cancer undergoing gastrectomy
Authors: Chen S , Zou Z , Chen F , Huang Z , Li G 
Published: July 2015

Introduction This meta-analysis evaluated the safety and efficacy of fast track surgery (FTS) for patients with gastric cancer undergoing gastrectomy. Methods Randomised controlled trials (RCTs) published between 1 January 1995 and 21 June 2013 comparing FTS with conventional perioperative care for patients with gastric cancer undergoing gastrectomy were identified in the PubMed, Embase™ and Cochrane Library databases, and were analysed systematically using RevMan software (Nordic Cochrane Centre, Copenhagen, Denmark). Results Seven RCTs (524 patients) were analysed. Compared with conventional perioperative care, FTS treatment with/without laparoscopy was associated with shorter postoperative hospitalisation, less hospitalisation expenditure (both p<0.00001), less pain and better quality of life. Short-term morbidity and readmission rates did not differ between treatments. No incidents of death occurred during the short-term follow-up period. Conclusions In patients with gastric cancer undergoing gastrectomy, the FTS pathway reduces the length and cost of postoperative hospitalisation while maintaining short-term morbidity, readmission and mortality rates comparable with those of conventional care.
Biodiversity And Dynamics Of Occurence Of Epigeic Groups In Different Types Of Farming
Authors: Porhajašová Jana , Noskovič Jaroslav , Rakovská Alena , Babošová Mária , Čeryová Terézia 
Published: July 2015

Abstract
The aim of this work was to determine and compare the occurrence of epigeic groups in two methods of farming, ecological one and integrated one. The research was conducte in the locality Nitra – Dolná Malanta in the years 2013 and 2014. The monitoring locality is situated in the south-western part of Slovakia, in altitude 175–180 m on highly productive soils. For the collection of biological material, the earth traps method was applied, used during the vegetation period (from April to October), within both farming systems, at Hordeum sativum, Triticum aestivum and Vicia faba undersowing with Medicago sativa. In canopy of these crops, two soil traps were installed, renewed in monthly intervals. The total of 7,722 exemplars of epigeic groups was obtained, of which 4,355 exemplars were in ecological farming and 3,367 exemplars in integrated farming system. In both treatments, 19 epigeic groups were determined, with dominant abundance of Coleoptera, Collembola, Acarina, Araneae. Also other groups such as Diplopoda, Heteroptera, Chilopoda etc. were observed in lower occurrence. Based on the evaluation of influence of the crop in terms of the occurrence of epigeic groups, the most suitable conditions created Vicia faba with undersowing Medicago sativa (integrated farming) and Triticum aestivum (ecological farming). On the basis of calculated indexes, both farming systems can be evaluated as homeostatically balanced, providing present epigeic groups with topical and trophic conditions.
Application of divergence entropy to characterize the structure of lipid-binding proteins
Authors: Rosicka Roksana , Banach Mateusz , Roterman-Konieczna Irena 
Published: July 2015

Abstract
The lipid-binding protein present in the human brain is the object of this analysis. The expression of these proteins is especially important for nervous cell differentiation and their migration in the process of the development of the brain. The “fuzzy oil drop” model applied to the analysis of these proteins may suggest the mechanism of complex generation. It is shown that this type of complex may appear spontaneously in water environment. The presence of ligand does not imply any form of adaptation of the polypeptide chain to the ligand molecule. It can be speculated that ligand binding is of a static character without the necessity for mutual structural fitting. The structures of polypeptide in the apo- and complexed forms do not differ in respect to hydrophobic core formation. Such an interpretation is different than that observed in other ligand-binding proteins where the binding cavity needs to be specially fitted to the specific ligand. It can also be concluded that the lipid-binding process is of low specificity in this case.
Transdisciplinary Methodology of the Dialogue Between People, Cultures, and Spiritualities
Authors: Nicolescu Basarab 
Published: July 2015

Abstract
When two people try to communicate there is, inevitably, confrontation: representation against representation, subconscious against subconscious. As this confrontation is subconscious, it often degenerates into conflict. A new model of civilisation is necessary, whose keystone is the dialogue between human beings, nations, cultures and religions for the survival of humanity. Inthe formation of a new model of civilization, the methodology of transdisciplinarity is crucial.
Ştefan Aug. Doinaş and Basarab Nicolescu, Epistolary Exchange and Aesthetic Transfiguration of Certain Transdisciplinary Concepts
Authors: Chețan Maria 
Published: July 2015

Abstract
Ştefan Aug. Doinaş and Basarab Nicolescu, two great spirits related through the generosity of the humanist vision, met, held an epistolary dialogue and had common projects. Doinaş commented upon a few of the innovative concepts proposed by Basarab Nicolescu and he also aesthetically transfigured, in literary pages, certain concepts of transdisciplinarity.
Assessment of Kwara State Social Studies Teachers of the Adequacy of Upper Basic Social Studies Curriculum Content for Sustainable Development in Nigeria
Authors: Yusuf A. , Daramola D.S. , Bello M.B. , Obafemi K.E. 
Published: July 2015

Abstract
The purpose of the study was to find out the adequacy of upper basic Social Studies curriculum content for sustainable development in Nigeria as assessed by Social Studies teachers. 306 Social Studies teachers (109 male and 197 female teachers) from 341 upper basic schools in Kwara State participated in the study. A researcher-designed questionnaire was used to collect data that were analysed using descriptive statistics and chi-square. The results showed that the content of upper basic Social Studies was not adequate, as assessed by teachers. It was also revealed that teachers’ assessment was significantly influenced by teaching experience, qualification and school type. However, the research showed that gender did not significantly influence Social Studies teachers’ assessment. Based on the findings, it was suggested that upper basic Social Studies curriculum should be reviewed and taught so as to ensure sustainable development in Nigeria.
Determination of rheological behaviour of wine lees
Authors: Lachman Jaromír , Rutkowski Kazimierz , Trávníček Petr , Vítěz Tomáš , Burg Patrik , Turan Jan , Junga Petr , Višacki Vladimir 
Published: July 2015

Abstract
This study deals with the rheological properties of wine lees. Samples of wine lees of the Saint Laurent variety were used in this experiment. The investigated wine lees arose in the process of production red grape wine in 2013 (Czech Republic). At first, the chemical background was determined. The chemical background includes the following chemical parameters: total acidity, pH, alcohol content, reduced sugars, free SO2, total SO2, and volatile acid. In the second phase of the study, physical properties were determined. Specifically, a sample of wine lees was subjected to rheological tests. These tests consisted in determination of dependence of dynamic viscosity on the temperature, hysteresis loop tests, dependence of dynamic viscosity on the time and step-down in the shear rate test. The experiment demonstrated that the dynamic viscosity increased with increased temperature – this phenomenon is caused by thermolabile proteins – and the wine lees has rheopectic behaviour; the degree of rheopexy was found to rise with the increasing temperature.
Effect of tannery wastes and biochar on soil chemical and physicochemical properties and growth traits of radish
Authors: Nabavinia Fereshteh , Emami Hojat , Astaraee Alireza , Lakzian Amir 
Published: July 2015

Abstract
Tannery wastes can be used in soil as organic manure. The aim of this research was to study the effects of tannery wastes (before a chromium addition step) and biochar on soil chemical and physicochemical properties and radish yield. Treatments included three levels of tannery waste (0, 2.5, and 5%) and two levels of biochar (0 and 2.5 t ha−1) in a completely randomized design (factorial) with three replications. The treated pots were kept under field capacity condition for three months; thereafter, five radish seeds were sown at 1 cm depth in soil of each pot. The results showed that tannery wastes and biochar treatments resulted in an increase in soil nitrogen and phosphorus contents and their concentrations in radish root and above ground biomass, but electrical conductivity of soil was decreased and had no effect on soil potassium content. The tannery waste treatment increased soil pH after plant harvest. It also significantly increased radish fresh and dry matter weight, but the biochar treatment yielded a slight increase. It is concluded that application of tannery wastes and biochar may be useful due to their high nutrient contents in soils lacking organic carbon under arid and semi-arid regions, thereby improving growth and increasing plant yield.