bluuebunny/crossref_metadata_2025_split huggingface dataset:
CSF1R inhibition depletes brain macrophages and reduces brain virus burden in SIV-infected macaques
Authors: Bohannon Diana G , Zablocki-Thomas Laurent D , Leung Evan S , Dupont Jinbum K , Hattler Julian B , Kowalewska Jolanta , Zhao Miaoyun , Luo Jiangtao , Salemi Marco , Amedee Angela M , Li Qingsheng , Kuroda Marcelo J , Kim Woong-Ki 
Published: July 2024

Abstract
Perivascular macrophages (PVMs) and, to a lesser degree, microglia are targets and reservoirs of HIV and simian immunodeficiency virus (SIV) in the brain. Previously, we demonstrated that colony-stimulating factor 1 receptor (CSF1R) in PVMs was upregulated and activated in chronically SIV-infected rhesus macaques with encephalitis, correlating with SIV infection of PVMs. Herein, we investigated the role of CSF1R in the brain during acute SIV infection using BLZ945, a brain-penetrant CSF1R kinase inhibitor.
Apart from three uninfected historic controls, nine Indian rhesus macaques were infected acutely with SIVmac251 and divided into three groups (n = 3 each): an untreated control and two groups treated for 20–30 days with low- (10 mg/kg/day) or high- (30 mg/kg/day) dose BLZ945. With the high-dose BLZ945 treatment, there was a significant reduction in cells expressing CD163 and CD206 across all four brain areas examined, compared with the low-dose treatment and control groups.
In 9 of 11 tested regions, tissue viral DNA (vDNA) loads were reduced by 95%–99% following at least one of the two doses, and even to undetectable levels in some instances. Decreased numbers of CD163+ and CD206+ cells correlated significantly with lower levels of vDNA in all four corresponding brain areas. In contrast, BLZ945 treatment did not significantly affect the number of microglia. Our results indicate that doses as low as 10 mg/kg/day of BLZ945 are sufficient to reduce the tissue vDNA loads in the brain with no apparent adverse effect. This study provides evidence that infected PVMs are highly sensitive to CSF1R inhibition, opening new possibilities to achieve viral clearance.
Differential reorganization of episodic and semantic memory systems in epilepsy-related mesiotemporal pathology
Authors: Cabalo Donna Gift , DeKraker Jordan , Royer Jessica , Xie Ke , Tavakol Shahin , Rodríguez-Cruces Raúl , Bernasconi Andrea , Bernasconi Neda , Weil Alexander , Pana Raluca , Frauscher Birgit , Caciagli Lorenzo , Jefferies Elizabeth , Smallwood Jonathan , Bernhardt Boris C 
Published: July 2024

Abstract
Declarative memory encompasses episodic and semantic divisions. Episodic memory captures singular events with specific spatiotemporal relationships, whereas semantic memory houses context-independent knowledge. Behavioural and functional neuroimaging studies have revealed common and distinct neural substrates of both memory systems, implicating mesiotemporal lobe (MTL) regions such as the hippocampus and distributed neocortices. Here, we explored declarative memory system reorganization in patients with unilateral temporal lobe epilepsy (TLE) as a human disease model to test the impact of variable degrees of MTL pathology on memory function.
Our cohort included 31 patients with TLE and 60 age- and sex-matched healthy controls, and all participants underwent episodic and semantic retrieval tasks during a multimodal MRI session. The functional MRI tasks were closely matched in terms of stimuli and trial design. Capitalizing on non-linear connectome gradient-mapping techniques, we derived task-based functional topographies during episodic and semantic memory states, in both the MTL and neocortical networks.
Comparing neocortical and hippocampal functional gradients between TLE patients and healthy controls, we observed a marked topographic reorganization of both neocortical and MTL systems during episodic memory states. Neocortical alterations were characterized by reduced functional differentiation in TLE across lateral temporal and midline parietal cortices in both hemispheres. In the MTL, in contrast, patients presented with a more marked functional differentiation of posterior and anterior hippocampal segments ipsilateral to the seizure focus and pathological core, indicating perturbed intrahippocampal connectivity. Semantic memory reorganization was also found in bilateral lateral temporal and ipsilateral angular regions, whereas hippocampal functional topographies were unaffected. Furthermore, leveraging MRI proxies of MTL pathology, we observed alterations in hippocampal microstructure and morphology that were associated with TLE-related functional reorganization during episodic memory. Moreover, correlation analysis and statistical mediation models revealed that these functional alterations contributed to behavioural deficits in episodic memory, but again not in semantic memory in patients.
Altogether, our findings suggest that semantic processes rely on distributed neocortical networks, whereas episodic processes are supported by a network involving both the hippocampus and the neocortex. Alterations of such networks can provide a compact signature of state-dependent reorganization in conditions associated with MTL damage, such as TLE.
State-dependent effects of responsive neurostimulation depend on seizure localization
Authors: Chiang Sharon , Khambhati Ankit N , Tcheng Thomas K , Loftman Audra Plenys , Hasulak Nicholas R , Mirro Emily A , Morrell Martha J , Rao Vikram R 
Published: July 2024

Abstract
Brain-responsive neurostimulation (RNS) is firmly ensconced among treatment options for drug-resistant focal epilepsy, but over a quarter of patients treated with the RNS® System do not experience meaningful seizure reduction. Initial titration of RNS therapy is typically similar for all patients, raising the possibility that treatment response might be enhanced by consideration of patient-specific variables. Indeed, small, single-centre studies have yielded preliminary evidence that RNS System effectiveness depends on the brain state during which stimulation is applied. The generalizability of these findings remains unclear, however, and it is unknown whether state-dependent effects of responsive neurostimulation are also stratified by location of the seizure onset zone where stimulation is delivered. We aimed to determine whether state-dependent effects of the RNS System are evident in the large, diverse, multi-centre cohort of RNS System clinical trial participants and to test whether these effects differ between mesiotemporal and neocortical epilepsies.
Eighty-one of 256 patients treated with the RNS System across 31 centres during clinical trials met the criteria for inclusion in this retrospective study. Risk states were defined in relation to phases of daily and multi-day cycles of interictal epileptiform activity that are thought to determine seizure likelihood. We found that the probabilities of risk state transitions depended on the stimulation parameter being changed, the starting seizure risk state and the stimulated brain region. Changes in two commonly adjusted stimulation parameters, charge density and stimulation frequency, produced opposite effects on risk state transitions depending on seizure localization. Greater variance in acute risk state transitions was explained by state-dependent responsive neurostimulation for bipolar stimulation in neocortical epilepsies and for monopolar stimulation in mesiotemporal epilepsies.
Variability in the effectiveness of RNS System therapy across individuals may relate, at least partly, to the fact that current treatment paradigms do not account fully for fluctuations in brain states or locations of simulation sites. State-dependence of electrical brain stimulation may inform the development of next-generation closed-loop devices that can detect changes in brain state and deliver adaptive, localization-specific patterns of stimulation to maximize therapeutic effects.
Nanoparticle Assisted Fabric Phase Sorptive Extraction for Azo Dye Determination in the Industrial Sewage
Authors: Rahimian Nayereh , Feizy Javad , Es’haghi Zarrin 
Published: July 2024

Abstract
Currently, one of the significant environmental problems is the presence of azo dye materials in water sources. In this study, for the first time, a fast and sensitive sample preparation approach using nanoparticle-assisted fabric phase sorptive extraction (NFPSE) followed by high-performance liquid chromatography was examined to remove some azo dyes such as methyl red and sunset yellow from aqueous solutions. Primarily, the significance of several parameters affecting NFPSE, such as fabric type, the kind of sorbent, the number of contacts with sol–gel and the time of contact, was investigated. In addition, experiments were performed to determine the effect of different adsorption parameters, such as sample volume, adsorption time, adsorbent value, desorption time, ionic strength and pH. It was found that the calibration curve was linear within two ranges of concentrations (0.05–0.1 and 0.5–15 ng/L for methyl red; 0.05–0.5 and 0.5–15 ng/L for sunset yellow) with correlation coefficients better than 0.9683. The limit of detection was 0.014 ng/L for methyl red and 0.015 ng/L for sunset yellow. Repeatability Relative Standard Deviation (RSD) with three replicated experiments was 1.5–10% for methyl red and 2.5–5.8% for sunset yellow. Relative recovery percentages of 88–96% for methyl red and 62–92% for sunset yellow were obtained in the samples. Moreover, the results have shown that acceptable accuracy, precision and linearity make the “fabric phase sorptive extraction” a proper method for the determination of dyes from industrial sewage samples.
Firms’ recruitment practices: the powers of segmentation and of valuation
Authors: de Larquier Guillemette , Rieucau Géraldine 
Published: July 2024

Abstract
This article proposes an original framework to show the plurality of matching dynamics, the processes by which firms and applicants meet and match in the labour market. It combines segmentation theory and economics of convention to characterise the powers of segmentation and valuation of firms when they recruit. Drawing on a national survey of French employers, the article identifies four matching dynamics. Thirty-five percent of the matches correspond to proximity-based matching dynamics, wherein firms do not control the candidates’ profiles. Thirty-four percent of matches result from standard dynamics and 17% from professional-based dynamics. In both cases, firms know the outline of the offer, evaluated through personalised criteria. Partnership-based matching dynamics (14% of the matches) are relatively open for candidates placed by intermediaries, whose evaluation is standardised. The article argues that no matching dynamic is more effective than others, and that employment policies must adapt to the plurality of labour market functioning.
Impact of peripheral thyroid hormone balance on liver fat: insights from the NutriAct trial
Authors: Sommer-Ballarini Miriam , Nguyen Thu-Huong , Pletsch-Borba Laura , Wernicke Charlotte , Tacke Frank , Schwerdtle Tanja , Pellowski Denny , Machann Jürgen , Spranger Joachim , Wirth Eva Katrin , Mai Knut 
Published: July 2024

Abstract

Objective
Hypothyroidism has been proposed as a potential contributor to steatotic liver disease (SLD), but existing data shows conflicting results in euthyroid subjects. Therefore, we investigated the association between thyroid function and intrahepatic lipids (IHLs) during a 36-month randomized controlled trial evaluating a diet known to reduce liver fat.

Design
502 eligible subjects (aged 50–80 years, ≥1 risk factor for unhealthy aging) were randomly assigned to either follow a diet rich in unsaturated fatty acids, plant protein, and fiber (intervention group, IG), or dietary recommendations of the German Nutrition Society (control group, CG).

Methods
Serum levels of thyroid hormones (THs) as well as IHLs, defined via magnetic resonance spectroscopy, were measured within an euthyroid subgroup without significant alcohol consumption at baseline (n = 332) and after 12 months (n = 243). A ratio of T3/T4 was used to assess whole-body deiodinase activity. Estimates of glucose and lipid metabolism were analyzed.

Results
Only fT3 and T3/T4 ratios showed a significant positive correlation with IHL at baseline. We observed a significant decline in fT3, T3, fT3/fT4 ratio, and T3/T4 ratio in CG and IG after 12 months without significant differences between groups. TSH, fT4, and T4 remained stable. A larger improvement of IHL during dietary intervention was seen in those subjects with a lower decline in T3 concentrations.

Conclusions
Altered TH balance indicates a possible compensatory upregulation of whole-body TH activity in subjects with increased liver fat. This might be also relevant during the improvement of hepatic steatosis.
The role of glucagon-like peptide 1 in the postprandial effects of metformin in type 2 diabetes: a randomized crossover trial
Authors: Hansen Laura S , Gasbjerg Lærke S , Brønden Andreas , Dalsgaard Niels B , Bahne Emilie , Stensen Signe , Hellmann Pernille H , Rehfeld Jens F , Hartmann Bolette , Wewer Albrechtsen Nicolai J , Holst Jens J , Vilsbøll Tina , Knop Filip K 
Published: July 2024

Abstract

Aims
Although metformin is widely used for treatment of type 2 diabetes (T2D), its glucose-lowering mechanism remains unclear. Using the glucagon-like peptide 1 (GLP-1) receptor (GLP-1R) antagonist exendin(9-39)NH2, we tested the hypothesis that postprandial GLP-1-mediated effects contribute to the glucose-lowering potential of metformin in T2D.

Methods
In a randomized, placebo-controlled, double-blind, crossover study, 15 individuals with T2D (median HbA1c 50 mmol/mol [6.7%], body mass index 30.1 kg/m2, age 71 years) underwent, in randomized order, 14 days of metformin and placebo treatment, respectively. Each treatment period was preceded by 14 days without any glucose-lowering medicine and concluded by two 4 h mixed meal tests performed in randomized order and separated by &gt;24 h with either continuous intravenous exendin(9-39)NH2 or saline infusion.

Results
Compared to placebo, metformin treatment lowered fasting plasma glucose (mean of differences [MD] 1.4 mmol/L × min [95% CI 0.8-2.0]) as well as postprandial plasma glucose excursions during both saline infusion (MD 186 mmol/L × min [95% CI 64-307]) and exendin(9-39)NH2 infusion (MD 268 mmol/L × min [95% CI 108-427]). The metformin-induced improvement in postprandial glucose tolerance was unaffected by GLP-1R antagonization (MD 82 mmol/L × min [95% CI −6564-170]). Metformin treatment increased fasting plasma GLP-1 (MD 1.7 pmol/L × min [95% CI 0.39-2.9]) but did not affect postprandial GLP-1 responses (MD 820 pmol/L × min [95% CI −1750-111]).

Conclusions
Using GLP-1R antagonization, we could not detect GLP-1-mediated postprandial glucose-lowering effect of metformin in individuals with T2D. We show that 2 weeks of metformin treatment increases fasting plasma GLP-1, which may contribute to metformin's beneficial effect on fasting plasma glucose in T2D.
Trial registration: Clinicaltrials.gov NCT03246451
Uterine changes in transgender men receiving testosterone therapy
Authors: da Silva Eliane Dias , Riveri Raquel Camara , Spritzer Poli Mara , Fighera Tayane Muniz 
Published: July 2024

Abstract

Objectives
Despite regular gender-affirming hormone therapy (GAHT), the presence of uterine bleeding can occur occasionally and cause profound discomfort. This study aimed to evaluate the histologic features and immunohistochemical expression of estrogen (ER), progesterone (PR), and androgen receptors (AR) in the endometrium and myometrium of transgender men receiving testosterone therapy and relate them to clinical and hormonal characteristics.

Design
Retrospective cross-sectional study.

Methods
Thirty-four transgender men undergoing gender-affirming surgery were included. Clinical, sociodemographic, and laboratory data as well as anatomopathological and immunohistochemical findings were evaluated.

Results
The participants’ mean age was 42.35 (SD, 10.00) years, and body mass index was 28.16 (SD, 5.52) kg/m2. The mean GAHT duration before surgery was 5.36 (SD, 3.24) years. The mean testosterone levels were 814.98 (SD, 407.13) ng/dL, and estradiol levels were 55.22 (SD, 25.27) pg/mL. The endometrium was atrophic in 61.8%, proliferative in 17.6%, and secretory in 20.6%. Immunohistochemical receptor analysis revealed that endometrial epithelial cells expressed ER (90%) and PR (80%), with a lower expression of AR (30%). In stromal tissue, the median ER, PR, and AR expression was lower than that in the epithelium (60%, 70%, and 25%, respectively). The myometrium showed high expression of PR (90%) and ER (70%), with the highest expression of AR (65%) being localized to this region.

Conclusions
In the present study, GAHT induced an atrophic condition of the endometrium in two-thirds of the transgender men, with a limited AR expression in the endometrial region. The present results suggest that testosterone-based GAHT for a mean of 5 years is safe in transgender men achieving amenorrhea.
May Measurement Month 2021: an analysis of blood pressure screening results from Thailand
Authors: Chotruangnapa Chavalit , Kunanon Sirisawat , Sukonthasarn Apichard , Srinonprasert Varalak , Beaney Thomas , Clarke Jonathan , Poulter Neil R , Roubsanthisuk Weranuj 
Published: July 2024

Abstract
Blood pressure (BP) measurement is the mainstay for diagnosing and treating hypertension. Blood pressure screening in the May Measurement Month (MMM) project is helpful for the early detection of hypertension, which is usually asymptomatic. This study aimed to investigate the epidemiology of hypertension from data in the MMM21 project in Thailand. A cross-sectional study was conducted according to the MMM clinical study protocol for MMM21 in all regions of Thailand from August to November 2021. We included adults aged ≥18 years. Baseline characteristics, history of hypertension diagnosis, and treatment were collected through the questionnaires followed by three standardized BP measurements, 1 min apart. We included 10 940 participants with a mean age of 41.3 (SD ± 13.5) years. Only 6% never had their BP measured before. Of all participants, 30.2% had hypertension, and among this hypertensive population, 50.3% were aware, and 46.2% were taking antihypertensive medications. Of participants with hypertension, 31.6% had controlled BP (&lt;140/90 mmHg), and BP control was 68.5% among those taking antihypertensive medication. The proportion of participants who had diabetes, were on aspirin, and were on statin were 6, 7.2, and 11.4%, respectively. Almost all participants (93.7%) had received at least one COVID-19 vaccination, and 8.0% reported a previous COVID-19 positive test. Most of the participants (97.4%) received hypertension care at no cost. Hypertension awareness and overall BP control rate were relatively low, while most subjects had previously had their BP checked. The widespread use of BP measurement may improve the diagnosis and treatment of hypertension to improve the care of those with hypertension in Thailand.
May Measurement Month 2021: an analysis of blood pressure screening results from Republic of the Congo, in the context of COVID-19 pandemic
Authors: Kouala Landa Christian-Michel , Bakekolo Paterne Rog , Makani-Bassakouahou Jospin , Ngolo Letomo Kivié Mou-Moué , Abena Exaucé , Macaire Batchi Dieuveil , Chocolat Raoul , Kwealeu Jean Pierre , Ngouloubi Offouma Gildas , Beaney Thomas , Telders Carolina Janssen , Poulter Neil R , Gombet Thierry Raoul , Ellenga Mbolla Bertrand 
Published: July 2024

Abstract
To determine the proportion of hypertension among opportunistic screenees in the Republic of the Congo, and the relationships between, blood pressure (BP), alcohol intake, and history of coronavirus disease 2019 (COVID-19). Screening was carried out in July and August 2021 in Brazzaville, Pointe-Noire, and Pokola, after ethical clearance had been obtained. The campaign followed the guidelines outlined in the MMM21 protocol, collecting ideally three BP readings for each participant. The screening was carried out during the partial confinement of the population, due to the COVID-19 pandemic. Social distancing measures and protection against the transmission of COVID-19 were adhered to. A total of 2135 subjects were screened of which 1138 (53.3%) were male. The mean age was 29.9 ± 9.9 years (range: 18–69 years), and 2110 participants (98.8%) were of black ethnic origin. A previous COVID-19 positive test was reported in 217 (10.2%) and vaccination in 93 (4.4%). Diabetes was reported in 113 (5.3%), 16 (0.7%) were current smokers, 171 (8.0%) reported using alcohol intake at least once per month, and 539 (25.3%) reported meeting the WHO guidelines on physical activity. The proportion of participants with hypertension (≥140/90 mmHg or taking BP-lowering medication) was 34.1%, of whom 24.5% were aware, 11.9% were on medication, and only 4.0% were controlled (&lt;140/90 mmHg). Rates of hypertension are high in the Republic of Congo, with low rates of awareness and treatment, and national authorities should address the contributing factors highlighted in this report to improve health care for the population.
Trajectories of satisfaction with work–family reconciliation among midlife employees: the role of family-related factors and quality of life
Authors: Neupane Subas , Lallukka Tea , Salonsalmi Aino , Haukka Eija , Leino-Arjas Päivi 
Published: July 2024

Abstract
We studied the developmental trajectories of satisfaction with work–family reconciliation (WFS) and their associations with family—related factors and quality of life measures among municipal employees. The study was based on the Helsinki Health Study of municipal employees of the City of Helsinki in 2001–02 and its follow-up surveys in 2007, 2012, and 2017. Employees aged 40–50 at baseline and working at all timepoints were analysed (n = 1681, 84% women). Growth Mixture Models were applied to identify trajectories of WFS (dissatisfied vs. satisfied). Associations of family-related and quality-of-life factors (physical functioning and emotional well-being) with the WFS trajectories were studied using log-binomial regression models, adjusting for sociodemographic and lifestyle variables. Two WFS trajectories, low (women 45%; men 53%) and high were identified. In a fully adjusted model among women, having ≥1 children aged 0–6 years was associated with increased odds of belonging to the low WFS trajectory (OR 1.52, 95% CI 1.19–1.95). Among men, having ≥1 children aged 7–18 was associated with decreased odds (0.39, 0.19–0.80). High emotional well-being was inversely associated with the low WFS trajectory among both genders (women 0.32, 0.23–0.45; men 0.20, 0.09-0.46). High physical functioning (0.59, 0.42–0.83) was inversely associated with the low WFS trajectory among women only. Less than half of the women and more than half of the men participants belonged to a low WFS trajectory, which associated with the age of children in the family and quality-of-life measures.