bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prediction of teicoplanin plasma concentration in critically ill patients: a combination of machine learning and population pharmacokinetics
Authors: Ma Pan , Shang Shenglan , Liu Ruixiang , Dong Yuzhu , Wu Jiangfan , Gu Wenrui , Yu Mengchen , Liu Jing , Li Ying , Chen Yongchuan 
Published: August 2024

Abstract

Background
Teicoplanin has been widely used in patients with infections caused by Staphylococcus aureus, especially for critically ill patients. The pharmacokinetics (PK) of teicoplanin vary between individuals and within the same individual. We aim to establish a prediction model via a combination of machine learning and population PK (PPK) to support personalized medication decisions for critically ill patients.

Methods
A retrospective study was performed incorporating 33 variables, including PPK parameters (clearance and volume of distribution). Multiple algorithms and Shapley additive explanations were employed for feature selection of variables to determine the strongest driving factors.

Results
The performance of each algorithm with PPK parameters was superior to that without PPK parameters. The composition of support vector regression, categorical boosting and a backpropagation neural network (7:2:1) with the highest R2 (0.809) was determined as the final ensemble model. The model included 15 variables after feature selection, of which the predictive performance was superior to that of models considering all variables or using only PPK. The R2, mean absolute error, mean squared error, absolute accuracy (±5 mg/L) and relative accuracy (±30%) of external validation were 0.649, 3.913, 28.347, 76.12% and 76.12%, respectively.

Conclusions
Our study offers a non-invasive, fast and cost-effective prediction model of teicoplanin plasma concentration in critically ill patients. The model serves as a fundamental tool for clinicians to determine the effective plasma concentration range of teicoplanin and formulate individualized dosing regimens accordingly.
Therapeutic effects of mitoquinol during an acute heat stress challenge in growing gilts
Authors: Mayorga Edith J , Freestone Alyssa D , Rudolph Tori E , Roths Melissa , Abeyta Megan A , Rodríguez-Jiménez Sonia , Goetz Brady M , Opgenorth Julie , Selsby Joshua T , Baumgard Lance H 
Published: August 2024

Abstract
Study objectives were to evaluate the effects of mitoquinol (MitoQ) on production parameters, gastrointestinal tract (GIT; stomach and small and large intestines) weight, and circulating leukocytes during a 24-h acute heat stress (HS) challenge. Crossbred gilts [n = 32; 49.1 ± 2.4 kg body weight (BW)] were blocked by BW and randomly assigned to 1 of 4 environmental-therapeutic treatments: 1) thermoneutral (TN) control (n = 8; TNCON), 2) TN and MitoQ (n = 8; TNMitoQ), 3) HS control (n = 8; HSCON), or 4) HS and MitoQ (n = 8; HSMitoQ). Pigs were moved into individual pens and allowed to acclimate for 6 d. The study consisted of 2 experimental periods (P). During P1 (2 d), all pigs remained in TN conditions (20.6 ± 1.5 °C) and were fed ad libitum. During P2 (24 h), pigs were fed ad libitum and exposed to either TN or constant HS (37.3 ± 1.3 °C). Mitoquinol (40 mg/d) was orally administered twice daily (0700 and 1800 hours) during P1 and P2. As expected, pigs exposed to HS had increased rectal temperature, skin temperature, and respiration rate (+1.5 °C, +8.7 °C, and +86 bpm, respectively; P &lt; 0.01) compared to their TN counterparts. Compared to TN, HS pigs had decreased feed intake (67%; P &lt; 0.01) and significant BW loss (+1.5 vs. −1.9 kg, respectively; P &lt; 0.01). Total GIT weight was decreased in HS relative to TN pigs (P &lt; 0.01), and this was influenced by decreased luminal contents (2.43 vs. 3.26 kg, respectively; P &lt; 0.01) and reduced empty GIT mass (3.21 vs. 3.48 kg, respectively; P = 0.03). Stomach contents remained similar between TN and HS pigs (P &gt; 0.54) but tended to increase in MitoQ relative to CON pigs (0.90 vs. 0.63 kg, respectively; P = 0.08). Stomach content as a percentage of the previous 24 h feed intake was increased in HS compared to the TN controls (93% vs. 31%; P &lt; 0.01). In contrast, small and large intestinal contents were decreased in HS compared to TN pigs (23% and 49%, respectively; P &lt; 0.01). Liver weight decreased in HS relative to TN pigs (1.15 vs. 1.22 kg, respectively; P = 0.02), and was decreased in MitoQ compared to CON pigs (1.13 vs. 1.24 kg; P &lt; 0.01). Circulating lymphocytes tended to be decreased in HS relative to TN pigs (16%; P = 0.07). In summary, acute HS increased all body temperature indices, negatively influenced animal performance, and differentially altered GIT motility as evidenced by decreased gastric emptying and increased intestinal transit. However, MitoQ supplementation did not appear to ameliorate these effects.
Eagle syndrome: tissue characteristics and structure of the styloid process
Authors: de Ruiter Ruben D , Treurniet Sanne , Bravenboer Nathalie , Busse Björn , Hendrickx Jan Jaap , Jansen Jeroen C , Dubois Leander , Schreuder Willem H , Micha Dimitra , Teunissen Bernd P , Raijmakers Pieter G H M , Eekhoff Elisabeth M W , von Brackel Felix N 
Published: August 2024

Abstract
Eagle syndrome is a bone disease where elongation of the styloid process leads to throat and neck pain, and in severe cases neurovascular symptoms such as syncope and neuralgia. The pathophysiology of Eagle syndrome is poorly understood with various theories having been proposed how this elongation is caused. To better understand the pathophysiology, we performed a work-up in 6 patients presenting with Eagle syndrome. Patients mainly presented with pain on turning the neck (100%), foreign body sensation (67%), tension in the neck (67%), and dysphagia (50%). The typical length of the styloid process ranges from 25 to 30 mm; however, [18F]NaF (sodium fluoride) PET/CT showed elongated styloid processes with an average length of 52.1 ± 15.6 mm (mean ± SD) with increased turnover at the base of one of the styloid processes. The removed styloid processes were further examined by histology, micro-CT, quantitative backscatter electron imaging (qBEI), Fourier transform infrared spectroscopy (FTIR), and circularly polarized light imaging. Histology revealed one case of a fractured styloid process healing through callus formation and one case of pseudarthrosis. Bone mineral density and mineralization was similar in the styloid processes when compared to cortical bone samples derived from the mandibular bone of different patients. Circular polarized light microscopy showed a collagen orientation in the styloid process comparable to the cortical bone samples with a distinct separation of collagen structure between the mineralized structure and the surrounding soft tissue with FTIR analysis demonstrating a typical composition of bone. This altogether suggests that the elongated styloid processes in Eagle syndrome are mature bone, capable of endochondral repair, possibly growing from the base of the process through endochondral ossification, rather than being a form of secondary calcification of the stylohyoid ligament as previously postulated.
Investigating pulmonary neutrophil responses to inflammation in mice via flow cytometry
Authors: Song Zhimin , Clemens Regina A , Zhang Yun , Chen Jingjing , Wang Yaofeng , Dinauer Mary C , Meng Shu 
Published: August 2024

Abstract
Neutrophils play a crucial role in maintaining lung health by defending against infections and participating in inflammation processes. Here we describe a detailed protocol for evaluating pulmonary neutrophil phenotype using a murine model of sterile inflammation induced by the fungal cell wall particle zymosan. We provide step-by-step instructions for the isolation of single cells from both lung tissues and airspaces, followed by comprehensive staining techniques for both cell surface markers and intracellular components. This protocol facilitates the sorting and detailed characterization of lung neutrophils via flow cytometry, making it suitable for downstream applications such as mRNA extraction, single-cell sequencing, and analysis of neutrophil heterogeneity. We also identify and discuss essential considerations for conducting successful neutrophil flow cytometry experiments. This work is aimed at researchers exploring the intricate functions of neutrophils in the lung under physiological and pathological conditions with the aid of flow cytometry.
CHST4 associates with high-abundance immune infiltration in hormone receptor–positive breast cancer
Authors: Liu Yansong , Hu Anbang , Shakila Suborna S , Cheng Weilun , Wang Ting , Zhang Jiarui , Yu Tianshui , Duan Yunqiang , Feng Jianyuan , Ding Yu , Zhang Hanyu , Li Yanling , Li Mingcui , Rong Zhiyuan , Shang Yuhang , Fang Zhengbo , Liu Jiangwei , Kong Fanjing , Guo Baoliang 
Published: August 2024

Abstract
Hormone receptor–positive breast cancer (HR+ BRCA) with high-risk factors such as lymph node metastasis has a relatively poor prognosis. However, the biological basis of tumor cell migration is still poorly understood, especially as some of the metastatic events occur at an early stage. Here, we identified that CHST4 (carbohydrate sulfotransferase 4), which has an important role in lymphocyte homing, was abnormally downregulated in HR+ BRCA and associated with lymph node metastasis. By enrichment analysis and immune infiltration evaluation, we predicted the potential ability of CHST4 to enhance immune cell infiltration. Then, immunohistochemical staining further demonstrated the contribution of CHST4 to the infiltration abundance of CD8+ T cells and CD4+ T cells in HR+ BRCA. Immunohistochemical staining of MECA-79 further identified the correlation between CHST4 and sulfated peripheral node addressin. Finally, we demonstrated that CHST4 was connected to increased tumor-immune cell communication by analyzing single-cell sequencing data. In summary, our study provided novel insights into the regulation of HR+ BRCA immune infiltration by CHST4.
Predation risk for hoary marmots in Washington’s North Cascades Mountains
Authors: Whiles Logan , Shipley Lisa A , Akins Jocelyn R , Christophersen Roger G , Ransom Jason I , Levi Taal , Thornton Daniel H 
Published: August 2024

Abstract
Declines in populations of small mammals associated with high elevations, e.g., marmots (Marmota spp.) and pikas (Ochotona spp.), have been attributed to both direct and indirect effects of environmental changes caused by humans. For example, populations of Olympic marmots (M. olympus) and Vancouver Island marmots (M. vancouverensis) have declined in response to increased predator access to high-elevation marmot habitats. In the North Cascades National Park Service Complex (NOCA), observed mean abundance of hoary marmots (M. caligata) declined by 74% from 2007 to 2016. Although these declines have been linked to harsh winter conditions, the role of predation and its association with decreasing snowpack has yet to be explored in this system. We noninvasively examined these predator–prey dynamics by coupling behavioral surveys of hoary marmots with indices of predation risk. We conducted 145 vigilance samples and 39 flight initiation distance trials to measure Hoary Marmot antipredator behavior. We derived a risk index for each predator by weighting its probability of use estimates (the probability of a predator–prey encounter) with its observed proportion of Hoary Marmot predation (probability of prey death given an encounter). We used genetic metabarcoding of vertebrate DNA from 413 predator scat samples to quantify predation on hoary marmots. We surveyed 9,930 trap-nights using 130 remote cameras to model predator probability of use near Hoary Marmot colonies. From camera traps and scat DNA, we detected 10 predator species in the study area, and we detected hoary marmots in the scats of 5 of these species. The proportion of observed Hoary Marmot predation was highest for coyotes (Canis latrans) at 50%. To our knowledge, we also report the first record of hoary marmots being consumed by Pacific martens (Martes caurina), which were also significant predators (proportion of observed predation = 32%). We expected predators with low-elevation habits to use sites with lesser snow persistence; this prediction was supported for coyotes but not for other typically low-elevation predators. We found estimated risk experienced by hoary marmots was highest from Pacific martens and lowest from Canada Lynx (Lynx canadensis). Contrary to our predictions and despite hoary marmots allocating &gt;50% of their time to vigilance, neither estimates of predator risk nor human presence explained variation in marmot antipredator behavior. Based on the results from both our dietary and probability of use analyses, we expect coyotes to have an increasing effect on NOCA’s Hoary Marmot population as the climate warms and Coyote range continues to expand. Thus, our work highlights the need to better understand the effect of climate-driven shifts in predator–prey ecology in high-elevation systems.
Rheumatoid arthritis associated vasculitis: a rare entity; case and review
Authors: Campos Ramos Marco A , Chao Zefr , Orozco Ronald , Reiter Kim , Glass Joseph , Vigil Anthony 
Published: August 2024

Abstract
We present a case of a 60-year-old male with known seropositive rheumatoid arthritis and cerebral vasculitis who presented to the emergency room with abrupt onset lower back and abdominal pain. The patient developed peritonitis which led to an abdominal laparotomy where jejunal ischemia, necrosis, and perforation were found, requiring bowel resection. On pathology examination, the patient had mesenteric vessel intramural inflammation indicative of vasculitis. He developed an anastomotic leak on postoperative Day 4 and elected hospice care. A high index of suspicion for mesenteric vasculitis should be considered in patients presenting with abdominal pain in the setting of known rheumatoid arthritis associated vasculitis, especially patients with long-standing rheumatoid arthritis. The high mortality represented by gastrointestinal involvement in rheumatoid arthritis associated vasculitis warrants investigation in high-risk patients, despite its low prevalence. Treatment may consist of high-dose corticosteroids, immunosuppressive agents, biologic therapies that target the underlying autoimmune process, and in severe cases, bowel resection.
TOI-2379 b and TOI-2384 b: two super-Jupiter mass planets transiting low-mass host stars
Authors: Bryant Edward M , Bayliss Daniel , Hartman Joel D , Sedaghati Elyar , Hobson Melissa J , Jordán Andrés , Brahm Rafael , Bakos Gaspar Á , Almenara Jose Manuel , Barkaoui Khalid , Bonfils Xavier , Cointepas Marion , Collins Karen A , Dransfield Georgina , Evans Phil , Gillon Michaël , Jehin Emmanuël , Murgas Felipe , Pozuelos Francisco J , Schwarz Richard P , Timmermans Mathilde , Watkins Cristilyn N , Wünsche Anaël , Butler R Paul , Crane Jeffrey D , Shectman Steve , Teske Johanna K , Charbonneau David , Essack Zahra , Jenkins Jon M , Lewis Hannah M , Seager Sara , Ting Eric B , Winn Joshua N 
Published: August 2024

ABSTRACT
Short-period gas giant planets have been shown to be significantly rarer for host stars less massive than the Sun. We report the discovery of two transiting giant planets – TOI-2379 b and TOI-2384 b – with low-mass (early M) host stars. Both planets were detected using Transiting Exoplanet Survey Satellite photometry and for both the transit signal was validated using ground-based photometric facilities. We confirm the planetary nature of these companions and measure their masses using radial velocity observations. We find that TOI-2379 b has an orbital period of 5.469 d and a mass and radius of $5.76\pm 0.20$ M$_{J}$ and $1.046\pm 0.023$ R$_{J}$ and TOI-2384 b has an orbital period of 2.136 d and a mass and radius of $1.966\pm 0.059$ M$_{J}$ and $1.025\pm 0.021$ R$_{J}$. TOI-2379 b and TOI-2384 b have the highest and third highest planet-to-star mass ratios, respectively, out of all transiting exoplanets with a low-mass host star, placing them uniquely among the population of known exoplanets and making them highly important pieces of the puzzle for understanding the extremes of giant planet formation.
Conflict as a cause of migration
Authors: Crippa Andrea , d’Agostino Giorgio , Dunne John Paul , Pieroni Luca 
Published: August 2024

Abstract
This article provides an empirical analysis of the relation between conflict and migration across a range of countries for which data are available and accounts both for the direct impact of conflict on migration and the indirect impact through its effect on economic opportunity. A model of migration is developed, where conflict affects wages and so migration decisions. The model is operationalized and estimated using data from the UN International Migration Stock and World Development Indicators and the International Country Risk Guide. This allows internal conflict to be measured as a continuous variable and its effect on net migration to be evaluated and the direct and indirect channels identified. A significant effect of conflict on net migration is found for low-income countries and it is shown to be robust to changes in the measurement of conflict and estimation methods.
Emotion-enriched moral perception
Authors: Hutton James 
Published: August 2024

Abstract
This article provides a new account of how moral beliefs can be epistemically justified. I argue that we should take seriously the hypothesis that the human mind contains emotion-enriched moral perceptions, i.e. perceptual experiences as of moral properties, arising from cognitive penetration by emotions. Further, I argue that if this hypothesis is true, then such perceptual experiences can provide regress-stopping justification for moral beliefs. Emotion-enriched moral perceptions do exhibit a kind of epistemic dependence: they can only justify moral beliefs if the emotions from which they arise are themselves justified. However, to have a justified emotion, one only needs (1) to possess some non-moral information and (2) to respond fittingly to this information. Neither (1) nor (2) requires one to possess any justification for moral beliefs antecedently, so emotion-enriched moral perceptions can halt the regress of moral justification.
Social Signaling and Childhood Immunization: A Field Experiment in Sierra Leone
Authors: Karing Anne 
Published: August 2024

Abstract
This article explores the use of social signaling as a policy tool to sustainably affect childhood immunization. In a 26-month field experiment with public clinics in Sierra Leone, I introduce a verifiable signal—in the form of color-coded bracelets—given to children upon timely completion of the first four or all five required vaccinations. Signals increase parents’ belief in the visibility of their actions and knowledge of other children’s vaccine status. The impact of signals varies significantly with the cost and perceived benefits of the action. There are no discernible effects on timely and complete immunization when the signal is linked to an easier-to-complete vaccine with low perceived benefits, and large positive effects when the signal is linked to a costlier-to-achieve vaccine with high perceived benefits. Parents adjust their behavior nine months before realizing the social image benefit, demonstrating the motivational strength of signaling incentives. Of substantive policy importance, bracelets increase full immunization at one year of age by 9 percentage points, with impacts persisting at two years of age. At a marginal cost of US$24.7 per fully immunized child, social signals can be as cost-effective as financial or in-kind incentives.
Neurostructural, Neurofunctional, and Clinical Features of Chronic, Untreated Schizophrenia: A Narrative Review
Authors: Tang Biqiu , Yao Li , Strawn Jeffrey R , Zhang Wenjing , Lui Su 
Published: August 2024

Abstract
Studies of individuals with chronic, untreated schizophrenia (CUS) can provide important insights into the natural course of schizophrenia and how antipsychotic pharmacotherapy affects neurobiological aspects of illness course and progression. We systematically review 17 studies on the neuroimaging, cognitive, and epidemiological aspects of CUS individuals. These studies were conducted at the Shanghai Mental Health Center, Institute of Mental Health at Peking University, and Huaxi MR Research Center between 2013 and 2021. CUS is associated with cognitive impairment, severe symptoms, and specific demographic characteristics and is different significantly from those observed in antipsychotic-treated individuals. Furthermore, CUS individuals have neurostructural and neurofunctional alterations in frontal and temporal regions, corpus callosum, subcortical, and visual processing areas, as well as default-mode and somatomotor networks. As the disease progresses, significant structural deteriorations occur, such as accelerated cortical thinning in frontal and temporal lobes, greater reduction in fractional anisotropy in the genu of corpus callosum, and decline in nodal metrics of gray mater network in thalamus, correlating with worsening cognitive deficits and clinical outcomes. In addition, striatal hypertrophy also occurs, independent of antipsychotic treatment. Contrasting with the negative neurostructural and neurofunctional effects of short-term antipsychotic treatment, long-term therapy frequently results in significant improvements. It notably enhances white matter integrity and the functions of key subcortical regions such as the amygdala, hippocampus, and striatum, potentially improving cognitive functions. This narrative review highlights the progressive neurobiological sequelae of CUS, the importance of early detection, and long-term treatment of schizophrenia, particularly because treatment may attenuate neurobiological deterioration and improve clinical outcomes.
Designing for data sharing: Considerations for advancing health equity in data management and dissemination
Authors: Rabin Borsika A , Smith Justin D , Dressler Emily V , Cohen Deborah J , Lee Rebekka M , Goodman Melody S , D’Angelo Heather , Norton Wynne E , Oh April Y 
Published: August 2024

Abstract
Data sharing, the act of making scientific research data available to others, can accelerate innovation and discoveries, and ultimately enhance public health. The National Cancer Institute Implementation Science Centers in Cancer Control convened a diverse group of research scientists, practitioners, and community partners in three interactive workshops (May–June 2022) to identify and discuss factors that must be considered when designing research for equitable data sharing with a specific emphasis on implementation science and social, behavioral, and population health research. This group identified and operationalized a set of seven key considerations for equitable data sharing—conceptualized as an inclusive process that fairly includes the perspectives and priorities of all partners involved in and impacted by data sharing, with consideration of ethics, history, and benefits—that were integrated into a framework. Key data-sharing components particularly important for health equity included: elevating data sharing into a core research activity, incorporating diverse perspectives, and meaningfully engaging partners in data-sharing decisions throughout the project lifecycle. As the process of data sharing grows in research, it is critical to continue considering the potential positive and adverse impact of data sharing on diverse beneficiaries of health data and research.