bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dependence of Cloud Macrophysical Properties and Phase Distributions on Environmental Conditions Over the North Atlantic and Southern Ocean: Results From COMBLE and MARCUS
Authors: Xia Zeqian , McFarquhar Greg M. 
Published: June 2024

AbstractThe accurate representation of Cold Air Outbreaks (CAOs) and affiliated mixed‐phase boundary layer (BL) clouds in models is challenging. How BL cloud properties evolve during CAOs and their dependence on meteorological conditions is not well understood but is important for the simulation of Earth's energy budgets. Here the properties of polar BL clouds over the North Atlantic (NA) and Southern Ocean (SO) are compared using observations from the Measurements of Aerosol Radiation and CloUds over the SO (MARCUS) and CAOs in the Marine BL Experiment (COMBLE) conducted over the NA. MARCUS observations show a stronger BL inversion than COMBLE, with a higher mean EIS (estimated inversion strength)/LTS (lower tropospheric stability) of −0.03 K/13 K compared to COMBLE’s −3.2 K/9.3 K. 39% of CAOs observed during COMBLE were intense with M > 5 K, while MARCUS only had 1.3%. 78%/72% of clouds sampled in CAOs during COMBLE/MARCUS had cloud top heights <4 km. The mean BL cloud top height was over 400 m higher, and the BL was over 500 m deeper for M of 10 K compared to 0 K for both regions. MARCUS observed a 27% moister BL structure than COMBLE when M > 5 K due to stronger BL inversion trapping more moisture within the BL. Under the same LTS, EIS, and M conditions, MARCUS observed a 12% drier BL structure, and clouds were 46% more turbulent than COMBLE. During CAOs, 54% of single‐layer BL clouds sampled during MARCUS had liquid‐dominated bases compared to 39% during COMBLE.
Contributions of Anthropogenic Greenhouse Gases and Aerosol Emissions to Changes in Summer Precipitation Over Southern China
Authors: Xu Huiwen , Chen Huopo , He Wenyue , Chen Lefeng 
Published: June 2024

AbstractThis study investigates the anthropogenic contribution to the summer precipitation changes over southern China and the underlying physical mechanisms. Observations show a wetting trend over southeastern China (SEC) but a drying trend over southwestern China (SWC) in summer of 1961–2014. The dipole pattern can be reasonably reproduced by the anthropogenic forcing simulations of CMIP6 models but with weak trends under the external natural forcing simulations, suggesting the vital human contribution to the observed changes. Particularly, anthropogenic greenhouse gases (GHG) dominate the wetting trend over SEC, while the drying trend over SWC is primarily attributed to anthropogenic aerosol (AA) emissions. Further analysis shows that the GHG concentrations enhance the subtropical high over the western North Pacific (WNP) via the heterogeneous warming of the sea surface temperature, decrease the sea level pressure over eastern China, and increase the atmospheric moisture, facilitating the moisture flux convergence (MFC) and the precipitation over SEC. The GHG‐induced wetting trend is somewhat offset by the inhibited evaporation due to the AA forcing. For SWC, the decreased precipitation is influenced by the anomalous high pressure from India to WNP, which is closely associated with the enhanced Asian AA emission and the interhemispheric asymmetrical distribution of AA emissions. In the upper troposphere, the uneven AA emissions between South and East Asia and Europe weaken the East Asian summer subtropical jet, resisting the western moisture to SWC. Both factors in the low‐and‐high levels suppress the MFC and precipitation over SWC, counteracted by the thermodynamical effects of GHG forcing.
Magma Chamber Depressurization and the Creation of Concentric Graben and Late‐Stage Flow Units at Sapas Mons, Venus
Authors: O’Hara Sean T. , McGovern Patrick J. , vonLembke Danielle 
Published: June 2024

AbstractSapas Mons is a large shield volcano in Atla Regio on Venus. Its summit region is partially encircled by a system of pits and extensional faults (graben). The existence and configuration of this system have been attributed to stresses generated above the margin of a magma chamber spanning the region beneath the summit. The proposed stress‐generating mechanism includes either withdrawal of magma from or solidification of magma within such a chamber (Keddie & Head, 1994, https://doi.org/10.1007/bf00644896). To explore these hypotheses, we calculate Finite Element Method models of stresses and deformations resulting from magma chamber depressurization beneath a Sapas Mons‐sized edifice with axisymmetric geometry. For a range of magma chamber depths and vertical thicknesses, we determine the minimum under pressure that produces a stress state predicting failure in circumferential normal mode at the observed position of the graben system. We also determine maximum under pressure under two conditions: (1) no failure (thrust fault mode) predicted at the summit, and (2) predicted failure (thrust fault mode) limited to within 10 km of the summit. We find that successful models require sill‐like chamber geometry with vertical thicknesses <1.5 km (diameter to thickness aspect ratios >66:1), and chamber depth <8 km beneath the summit. Calculated reductions in chamber volume are comparable to volumes of late‐stage eruptive units mapped at Sapas Mons, favoring the magma withdrawal hypothesis for graben system formation. Evidence that unit 5 of Keddie and Head (1994, https://doi.org/10.1007/bf00644896) overlapped in time with, but largely postdated, the graben forming event renders it the most likely destination for magma removed from the chamber.
Constraining Plio‐Pleistocene Shifts in Northwest African Hydroclimate, Ecosystem Distributions, and Marine Productivity: New Paleo‐Records Across the Mid‐Pleistocene Transition
Authors: O’Mara Nicholas A. , Skonieczny Charlotte , McGee David , Winckler Gisela , Bory Aloys J.‐M. , Bradtmiller Louisa I. , Malaizé Bruno , Polissar Pratigya J. 
Published: June 2024

AbstractNorthwest Africa transitioned from a wet/vegetated landscape toward drier/sparser conditions sometime between the late‐Pliocene and the late‐Pleistocene. However, our understanding of the precise timing and nature of this transition is hampered by a paucity of paleo‐records which bridge these two intervals. Here we report new plant‐wax isotope as well as dust and opal flux records from the relatively brief interval ∼1.1–1.0 million years ago (Ma) to evaluate the astronomical timescale controls of Northwest African hydroclimate and vegetation during the Mid‐Pleistocene Transition (MPT) and, in context with published records, the drivers of long‐term climate and ecological trends over the Plio‐Pleistocene. The tempo and amplitude of the Northwest African monsoon rainfall swings closely track low latitude insolation forcings over the last 5 Ma. However, we demonstrate that a pronounced mean state decline in monsoon strength likely occurred following the MPT most likely instigated by increasing Atlantic meridional sea surface temperature gradients or declines in the strength of the meridional overturning circulation. The northward extent of vegetation does not track changes in monsoon strength over the Plio‐Pleistocene and thus may be more strongly influenced by changes in monsoon rainfall extent or ecosystem disturbances. Progressively diminished dust fluxes following a decline in monsoon strength after 1.0 Ma is consistent with reduced production and subsequent depletion of fine‐grained sediments in the Sahara. Synchroneity between dust and opal fluxes across timescales suggests nutrient delivery to the surface ocean via dust plays a key role in marine primary productivity off the coast of Northwest Africa.
Structural Evolution, Exhumation Rates, and Rheology of the European Crust During Alpine Collision: Constraints From the Rotondo Granite—Gotthard Nappe
Authors: Ceccato A. , Behr W. M. , Zappone A. S. , Tavazzani L. , Giuliani A. 
Published: June 2024

AbstractThe rheology of crystalline units controls the large‐scale deformation geometry and dynamics of collisional orogens. Defining a time‐constrained rheological evolution of such units may help unravel the details of collisional dynamics. Here, we integrate field analysis, pseudosection calculations and in situ garnet U–Pb and mica Rb–Sr geochronology to define the structural and rheological evolution of the Rotondo granite (Gotthard nappe, Central Alps). We identify a sequence of four (D1–D4) deformation stages. Pre‐collisional D1 brittle faults developed before Alpine peak metamorphism, which occurred at 34–20 Ma (U–Pb garnet ages) at 590 ± 25°C and 0.9 ± 0.1 GPa. The reactivation of D1 structures controlled the rheological evolution, from D2 reverse mylonitic shearing at amphibolite facies (520 ± 40°C and 0.8 ± 0.1 GPa) at 18–20 Ma (white mica Rb–Sr ages), to strike‐slip, brittle‐ductile shearing at greenschist‐facies D3 (395 ± 25°C and 0.4 ± 0.1 GPa) at 14–15 Ma (white mica and biotite Rb–Sr ages), and then to D4 strike‐slip faulting at shallow conditions. Although highly misoriented for the Alpine collisional stress orientation, D1 brittle structures controlled the localization of D2 ductile mylonites accommodating fast (∼3 mm/yr) exhumation rates due to their weak shear strength (<10 MPa). This structural and rheological evolution is common across External Crystalline Massifs (e.g., Aar, Mont Blanc), suggesting that the European upper crust was extremely weak during Alpine collision, its strength controlled by weak ductile shear zones localized on pre‐collisional deformation structures, that in turn controlled localized exhumation at the scale of the orogen.
Crust‐Derived CO2 Production From a Shallow Pluton in Limestone Is Driven by Metamorphic Decarbonation, Not Assimilation
Authors: Morris R. A. , Canil D. 
Published: June 2024

AbstractTraditionally, it is assumed that contributions of crust‐derived CO2 emissions at arc settings are minor, but this requires well‐constrained field studies to determine the extent of magma‐carbonate reaction and concomitant C released. The Jurassic Bonanza arc on Vancouver Island (Canada) was built on a Triassic limestone platform and makes for an ideal setting to examine arc magma‐carbonate interactions beneath an island arc. We examine how km‐scale magma bodies might react with carbonate from a well‐constrained study of a gabbro pluton that intrudes limestone. The pluton shows muted to nil carbonate interaction preserving primary igneous 87Sr/86Sr values (∼0.703) except for a thin (<2 m wide) marginal gabbro (∼0.706) in contact with a decarbonized metamorphic aureole. A lack of 87Sr/86Sr or δ18Ocpx enrichment in gabbro ∼10 to >1,000 m from the contact suggests that any reaction with limestone wallrock is limited to at most the outer ∼10 m of the pluton. More enhanced magma‐carbonate interaction and CO2 production occurs via a network of shallow dikes and sills (<0.2 GPa) compared to deeper plutons, consistent with experimental data showing increased carbonate assimilation at shallower depths (≤0.5 GPa). Plutons are an important heat source to release CO2 from carbonate wallrock by contact metamorphism, where >89% of crust‐derived CO2 is liberated by wallrock decarbonation and <11% is liberated by magma that assimilated limestone. Nonetheless, we show that neither magmatic nor metamorphic crust‐derived CO2 contributions compare to mantle‐derived CO2. Our study places realistic and quantitative limits on arc‐derived CO2 from upper crustal limestone sources.
Evaluation of the Planetary Boundary Layer Height From ERA5 Reanalysis With MOSAiC Observations Over the Arctic Ocean
Authors: Xi Xingya , Yang Qinghua , Liu Changwei , Shupe Matthew D. , Han Bo , Peng Shijie , Zhou Shaohui , Chen Dake 
Published: June 2024

AbstractThe planetary boundary layer height (PBLH) is a crucial indicator reflecting the region of the atmosphere characterized by continuous turbulence. Here, we use radiosonde and surface meteorological observations (4–7 times per day, year‐round measurements) during the Multidisciplinary drifting Observatory for the Study of Arctic Climate (MOSAiC) expedition to derive the PBLH (PBLHMOSAiC), and further evaluate the PBLH from the ERA5 reanalysis (PBLHERA5). Comparisons between PBLHMOSAiC and PBLHERA5 from different perspectives reveal that: (a) The overestimation of PBLHERA5 when the sea ice concentration is >90% is significant with the centered root mean squared error reaching up to 201 m; (b) The difference between the two products is notably pronounced in cold seasons, while it is comparatively diminished in warm seasons; (c) In neutral boundary layers, differences in PBLHERA5 are larger compared with stable and convective boundary layers. In addition, the analysis of error sources indicates that the bias of PBLHERA5 is sensitive to the bias of vertical thermal structure and wind speed profiles in ERA5 data sets in all conditions. Finally, we find a Random Forest model effectively reduces the bias of PBLHERA5 with the index of agreement reaching up to 0.71 in the test data set, while a multiple linear regression demonstrates comparable performance to the Random Forest model.
Widespread occurrence of pesticides in low-income housing
Authors: Vaezafshar Sara , Siegel Jeffrey A. , Jantunen Liisa , Diamond Miriam L. 
Published: June 2024

Abstract
Background
Low socioeconomic status (SES) residents living in social housing, which is subsidized by government or government-funded agencies, may have higher exposures to pesticides used in indoor residences since pesticides are applied due to structural deficiencies, poor maintenance, etc.

Objective
To estimate exposure of residents in low-SES social housing built in the 1970s to legacy and current-use pesticides and to investigate factors related to exposures.

Methods
Twenty-eight particle-phase pesticides were measured in the indoor air of 46 units in seven low-income social housing, multi-unit residential buildings (MURBs) in Toronto, Canada using portable air cleaners deployed for 1 week in 2017. Pesticides analyzed were legacy and current use in the classes: organochlorines, organophosphates, pyrethroids, and strobilurins.

Results
At least one pesticide was detected in 89% of the units with detection frequencies (DF) for individual pesticides of up to 50%, including legacy organochlorines and current-use pesticides. Current-use pyrethroids had the highest DF and concentrations, with the highest particle-phase concentration for pyrethrin I at 32,000 pg/m3. Heptachlor, restricted for use in Canada in 1985, had the highest estimated maximum total air (particle plus gas phase) concentration of 443,000 pg/m3. Heptachlor, lindane, endosulfan I, chlorothalonil, allethrin, and permethrin (except in one study) had higher concentrations than those measured in low-income residences reported elsewhere. In addition to the intentional use of pesticides to control pests and their use in building materials and paints, tobacco smoking was significantly correlated with the concentrations of five pesticides used on tobacco crops. The distribution of pesticides with high DF in individual buildings suggested that pest eradication programs by the building management and/or pesticide use by residents were the major sources of measured pesticides.

Impact
Low-income social housing fills a much-needed demand, but the residences are prone to pest infestation and hence pesticide use. We found exposure to at least 1 of 28 particle-phase pesticides in 89% of all 46 units tested, with the highest DF and concentrations for current-use pyrethroids and long-banned organochlorines (e.g., DDT, heptachlor) due to very high persistence indoors. Also measured were several pesticides not registered for use indoors, e.g., strobilurins used to treat building materials and pesticides used on tobacco crops. These results, which are the first Canadian data for most pesticides indoors, show widespread exposure to numerous pesticides.
Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis
Authors: Kural Mehmet H. , Djakbarova Umidahan , Cakir Bilal , Tanaka Yoshiaki , Chan Emily T. , Arteaga Muniz Valeria I. , Madraki Yasaman , Qian Hong , Park Jinkyu , Sewanan Lorenzo R. , Park In-Hyun , Niklason Laura E. , Kural Comert 
Published: June 2024

AbstractThe transmembrane death receptor Fas transduces apoptotic signals upon binding its ligand, FasL. Although Fas is highly expressed in cancer cells, insufficient cell surface Fas expression desensitizes cancer cells to Fas-induced apoptosis. Here, we show that the increase in Fas microaggregate formation on the plasma membrane in response to the inhibition of endocytosis sensitizes cancer cells to Fas-induced apoptosis. We used a clinically accessible Rho-kinase inhibitor, fasudil, that reduces endocytosis dynamics by increasing plasma membrane tension. In combination with exogenous soluble FasL (sFasL), fasudil promoted cancer cell apoptosis, but this collaborative effect was substantially weaker in nonmalignant cells. The combination of sFasL and fasudil prevented glioblastoma cell growth in embryonic stem cell-derived brain organoids and induced tumor regression in a xenograft mouse model. Our results demonstrate that sFasL has strong potential for apoptosis-directed cancer therapy when Fas microaggregate formation is augmented by mechano-inhibition of endocytosis.
Overexpression of TBX3 suppresses tumorigenesis in experimental and human cholangiocarcinoma
Authors: Deng Shanshan , Lu Xinjun , Wang Xue , Liang Binyong , Xu Hongwei , Yang Doris , Cui Guofei , Yonemura Andrew , Paine Honor , Zhou Yi , Zhang Yi , Simile Maria Maddalena , Urigo Francesco , Evert Matthias , Calvisi Diego F. , Green Benjamin L. , Chen Xin 
Published: June 2024

AbstractTBX3 behaves as a tumor suppressor or oncoprotein across cancer. However, TBX3 function remains undetermined in intrahepatic cholangiocarcinoma (iCCA), a deadly primary liver malignancy with few systemic treatment options. This study sought to investigate the impact of TBX3 on iCCA. We found that overexpression of TBX3 strongly inhibited human iCCA cell growth. In the Akt/FBXW7ΔF mouse iCCA model, overexpression of Tbx3 reduced cholangiocarcinogenesis in vivo, while inducible genetic knockout of Tbx3 accelerated iCCA growth. RNA-seq identified MAD2L1 as a downregulated gene in TBX3-overexpressing cells, and ChIP confirmed that TBX3 binds to the MAD2L1 promoter. CRISPR-mediated knockdown of Mad2l1 significantly reduced the growth of two iCCA models in vivo. Finally, we found that TBX3 expression is upregulated in ~20% of human iCCA samples, and its high expression is associated with less proliferation and better survival. MAD2L1 expression is upregulated in most human iCCA samples and negatively correlated with TBX3 expression. Altogether, our findings suggest that overexpression of TBX3 suppresses CCA progression via repressing MAD2L1 expression.
USP14 deficiency inhibits neointima formation following vascular injury via degradation of Skp2 protein
Authors: Xia Xiaohong , Liu Xiaolin , Xu Qiong , Gu Jielei , Ling Sisi , Liu Yajing , Li Rongxue , Zou Min , Jiang Siqin , Gao Zhiwei , Chen Canshan , Liu Shiming , Liu Ningning 
Published: June 2024

AbstractUbiquitin-proteasome system (UPS) is involved in vascular smooth muscle cell (VSMC) proliferation. Deubiquitinating enzymes (DUBs) have an essential role in the UPS-regulated stability of the substrate; however, the function of DUBs in intimal hyperplasia remains unclear. We screened DUBs to identify a protein responsible for regulating VSMC proliferation and identified USP14 protein that mediates cancer development, inflammation, and foam cell formation. USP14 promotes human aortic smooth muscle cell and A7r5 cell growth in vitro, and its inhibition or deficiency decreases the intimal area in the mice carotid artery ligation model. In addition, USP14 stabilizes Skp2 expression by decreasing its degradation, while Skp2 overexpression rescues USP14 loss-induced issues. The current findings suggested an essential role of USP14 in the pathology of vascular remodeling, deeming it a promising target for arterial restenosis therapy.
DNA methylation as an epigenetic mechanism in the regulation of LEDGF expression and biological response in aging and oxidative stress
Authors: Bhargavan Biju , Chhunchha Bhavana , Kubo Eri , Singh Dhirendra P. 
Published: June 2024

AbstractThe physiological quantum of stress-inducible transcriptional protein, Lens Epithelium-Derived Growth Factor (LEDGF), is vital for the maintenance of cellular physiology. Erratic epigenetic reprogramming in response to oxidative stress or with advancing age is found to be a major cause in the gene silencing, leading to pathobiologies. Using aging human (h) eye lens/lens epithelial cells (LECs) coupled with redox-active Peroxiredoxin 6 (Prdx6)-deficient (Prdx6−/−) mLECs as model systems, herein, we showed that in aging/oxidative stress, the human LEDGF gene was regulated by unique methylation patterns of CGs nucleotides within and around the Sp1 binding site(s) of CpG island of the LEDGF promoter (−170 to −27nts). The process caused the repression of LEDGF and its target, Hsp27, resulting in reactive oxygen species (ROS) amplification and cellular insults. This phenomenon was opposed to the unmethylated promoter in LECs. Clinically, we observed that the loss of LEDGF in the Prdx6−/− mLECs or aging lenses/LECs, correlating with increased expression of DNMT1, DNMT3a, and DNMT3b along with the methyl CpG binding protein 2 (MeCP2). Upon oxidative stress, the expression of these molecules was increased with the dramatic reduction in LEDGF expression. While demethylating agent, 5-Aza deoxycytidine (5-AzaC) transposed the aberrant methylation status, and revived LEDGF and Hsp27 expression. Mechanistically, the chloramphenicol acetyltransferase (CAT) reporter gene driven by the LEDGF promoter (−170/ + 35) and ChIP assays uncovered that 5-AzaC acted on GC/Sp1 sites to release LEDGF transcription. The data argued, for the first time, that de novo methylation of CGs around and within Sp1 sites of the CpG island directly disrupted Sp1 activity, which ensued in LEDGF repression and its biological functions. The findings should improve our understanding of cellular insults-associated with aberrant DNMTs-mediated LEDGF’s activity, and can offer strategies for therapeutic intervention to halt aging/oxidative stress-induced abnormalities.