bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘Let other people do it…’: the role of emergency department nurses in health promotion
Authors: Shoqirat Noordeen 
Published: December 2013

Aims and objectivesTo explore emergency department nurses’ roles in health promotion and contributing factors involved.BackgroundIn many healthcare systems, emergency departments are increasingly urged to play a crucial role in health promotion. Although much has been written about health promotion and nurses in acute care settings, less has focused on emergency departments.DesignA qualitative design was used.MethodsIn‐depth semi‐structured interviews were used with a convenient sample of 15 nurses in a Jordanian emergency department. Data were analysed using nvivo 9 (QSR International, Doncaster, Victoria, Australia).ResultsThe analysis identified two themes. The first focused on nurses’ roles in health promotion, comprising two categories: having no roles and ‘let other people do it’. The second theme focused on the context and constraints of health promotion comprising fear of violence, the nature of healthcare service and patients’ beliefs. The majority of nurses in emergency departments do not associate health promotion with their practice, hold some negative attitudes and devote more time to clinical tasks than health promotion, which is seen as a second priority.ConclusionsThe ideology of medicalised tasks has penetrated deeply into the core principles of health promotion, and thus, nurses’ roles and potential in this area are questioned.Relevance to clinical practiceDecision‐makers and nurses within the hospital are urged to address the constraints identified in this study and debate them further. Failure to do so may lead to emergency nursing not being optimally achieved, with the absence of sustained and concerted health promotion work matching patients’ cultural needs and sensitivities.
α‐Smooth muscle actin‐positive myofibroblasts, in association with epithelial–mesenchymal transition and lymphogenesis, is a critical prognostic parameter in patients with oral tongue squamous cell carcinoma
Authors: Ding Lei , Zhang Ziwen , Shang Duo , Cheng Jie , Yuan Hua , Wu Yunong , Song Xiaoling , Jiang Hongbing 
Published: December 2013

Backgroundα‐Smooth muscle actin (α‐SMA)‐positive myofibroblasts play a pivotal role in progression and metastasis of solid carcinomas. Epithelial–mesenchymal transition (EMT) of cancer cells and lymphogenesis of tumor microenvironment are the important events in tumor metastasis. This study aimed to investigate the relationship between the expression of myofibroblasts marker, α‐SMA, and clinicopathological features, EMT, lymphogenesis, and prognostic status in oral tongue squamous cell carcinoma (OTSCC).MethodsImmunohistochemisty was used to detect α‐SMA expression in 50 OTSCCs. EMT and lymphogenesis were also identified by immunostaining with N‐cadherin, vimentin, and lymphatic vessel endothelial hyaluronan receptor 1 (LYVE‐1).ResultsThere was a significant correlation respectively between the α‐SMA (P = 0.002), vimentin (P < 0.001), N‐cadherin (P = 0.025) expression and cervical lymph node metastasis of OTSCC. Carcinomas with α‐SMA (P = 0.001), vimentin (P = 0.003), and N‐cadherin (P = 0.012) expression were more advanced in terms of tumor‐node‐metastases status. Univariate analysis showed that pathologic node status (P < 0.001), α‐SMA (P = 0.001), and vimentin expression (P = 0.044) was significantly associated with overall survival time, but multivariate analysis just showed the α‐SMA expression (P = 0.008) and pathologic node status (P = 0.003) was independently predictive of prognosis. Furthermore, statistical analysis showed significant correlation between α‐SMA expression and vimentin (P = 0.037), N‐cadherin (P = 0.019), or LYVE‐1 positive vessel count (P = 0.041).ConclusionOur results indicate that α‐SMA‐positive myofibroblasts have important impacts on cancer progression, metastasis, and survival prognosis of patients with OTSCC. The functions of α‐SMA‐positive myofibroblasts in OTSCC may be associated with promoting EMT of tumor cells and lymphogenesis of metastasis microenvironment.
Measuring individual vulnerability to sleep loss—the CHICa scale
Authors: Oginska Halszka , Mojsa‐Kaja Justyna , Fafrowicz Magdalena , Marek Tadeusz 
Published: December 2013

SummaryThe aim of this project was to construct a psychometrically satisfying scale to describe subjective reactions to sleep deprivation. First, on the basis of a literature review, a list of items was generated which reflected the negatively affected mood and reduced wellbeing associated with sleep loss. Additionally, psychology students were asked to describe their cognitive and emotional symptoms following a night with curtailed sleep. As a result, 69 items were included in the experimental set. University students (n = 102, females, mean age 22.5 ± 1.9 years) completed the form several times during 1 week in June (while preparing for examinations) and on a free day in September; a total of 460 forms were collected. The final, 26‐item version of the scale was validated in a sleep deficit experiment lasting 1 week, conducted with 25 female participants (mean age 23.4 ± 1.9 years). Factor analysis showed 71.7% of total variance explained by four components: impaired thermoregulation (C for cold), disrupted appetite (H for hunger), affective problems (I for irritability) and lowered level of cognitive functioning (Ca for cognitive attenuation). A Polish version of the CHICa scale showed satisfactory psychometric properties. Cronbach's alpha of the subscales were between 0.90 and 0.95. All four subscales exhibited a significant increase with an experimental 3‐h daily sleep restriction over a period of 1 week; cognitive attenuation was the most symptomatic. Cognitive problems (reduced concentration, comprehension and accuracy) and a lack of energy seem to be the most specific subjective manifestations of the chronic sleep deficit state. CHICa may be helpful in research on inter‐and intra‐individual differences and on the efficacy of various counteractive treatments for the consequences of sleep deprivation.
Cathodoluminescence microscopy and spectroscopy of forsterite from Kaba meteorite: An application to the study of hydrothermal alteration of parent body
Authors: Gucsik Arnold , Endo Taro , Nishido Hirotsugu , Ninagawa Kiyotaka , Kayama Masahiro , Bérczi Szaniszló , Nagy Szabolcs , Ábrahám Péter , Kimura Yuki , Miura Hitoshi , Gyollai Ildikó , Simonia Irakli , Rózsa Péter , Posta József , Apai Dániel , Mihályi Krisztián , Nagy Mihály , Ott Ulrich 
Published: December 2013

AbstractHighly forsteritic olivine (Fo: 99.2–99.7) in the Kaba meteorite emits bright cathodoluminescence (CL). CL spectra of red luminescent forsterite grains have two broad emission bands at approximately 630 nm (impurity center of divalent Mn ions) in the red region and above 700 nm (trivalent Cr ions) in the red–IR region. The cores of the grains show CL blue luminescence giving a characteristic broad band emission at 400 nm, also associated with minor red emissions related to Mn and Cr ions. CL color variation of Kaba forsterite is attributed to structural defects. Electron probe microanalyzer (EPMA) analysis shows concentrations of Ca, Al, and Ti in the center of the forsterite grain. The migration of diffusible ions of Mn, Cr, and Fe to the rim of the Kaba meteoritic forsterite was controlled by the hydrothermal alteration at relatively low temperature (estimated at about 250 °C), while Ca and Al ions might still lie in the core. A very unusual phase of FeO (wüstite) was also observed, which may be a terrestrial alteration product of FeNi‐metal.
Split Sample Methods for Constructing Confidence Intervals for Binomial and Poisson Parameters
Authors: Decrouez Geoffrey , Hall Peter 
Published: December 2013

SummaryWe introduce a new method for improving the coverage accuracy of confidence intervals for means of lattice distributions. The technique can be applied very generally to enhance existing approaches, although we consider it in greatest detail in the context of estimating a binomial proportion or a Poisson mean, where it is particularly effective. The method is motivated by a simple theoretical result, which shows that, by splitting the original sample of size n into two parts, of sizes n1 and n2=n−n1, and basing the confidence procedure on the average of the means of these two subsamples, the highly oscillatory behaviour of coverage error, as a function of n, is largely removed. Perhaps surprisingly, this approach does not increase confidence interval width; usually the width is slightly reduced. Contrary to what might be expected, our new method performs well when it is used to modify confidence intervals based on existing techniques that already perform very well—it typically improves significantly their coverage accuracy. Each application of the split sample method to an existing confidence interval procedure results in a new technique.
On Semiparametric Inference of Geostatistical Models via Local Karhunen–Loève Expansion
Authors: Chu Tingjin , Wang Haonan , Zhu Jun 
Published: December 2013

SummaryWe develop a semiparametric approach to geostatistical modelling and inference. In particular, we consider a geostatistical model with additive components, where the form of the covariance function of the spatial random error is not prespecified and thus is flexible. A novel, local Karhunen–Loève expansion is developed and a likelihood-based method is devised for estimating the model parameters and statistical inference. A simulation study demonstrates sound finite sample properties and a real data example is given for illustration. Finally, the theoretical properties of the estimates are explored and, in particular, consistency results are established.
Angiostatic Effects of NK Cell‐Derived IFN‐γ Counteracted by Tumour Cell Bcl‐xL Expression
Authors: Wallin R. P. A. , Sundquist V. S. , Bråkenhielm E. , Cao Y. , Ljunggren H.‐G. , Grandien A. 
Published: December 2013

AbstractAnti‐apoptotic proteins that block death receptor‐mediated apoptosis favour tumour evasion of the immune system, leading to enhanced tumour progression. However, it is unclear whether blocking the mitochondrial pathway of apoptosis will protect tumours from immune cell attack. Here, we report that the anti‐apoptotic protein Bcl‐xL, known for its ability to block the mitochondrial pathway of apoptosis, exerted tumour‐progressive activity in a murine lymphoma model. Bcl‐xL overexpressing tumours exhibited a more aggressive development than control tumours. Surprisingly, Bcl‐xL protection of tumours from NK cell‐mediated attack did not involve protection from NK cell‐mediated cytotoxicity. Instead, Bcl‐xL‐blocked apoptosis resulting from hypoxia and/or nutrient loss associated with the inhibition of angiogenesis caused by NK cell‐secreted IFN‐γ. These results support the notion that NK cells may inhibit tumour growth also by mechanisms other than direct cytotoxicity. Hence, the present results unravel a pathway by which tumours with a block in the mitochondrial pathway of apoptosis can evade the immune system.
Pretransplant C‐reactive Protein as A Prognostic Marker in Allogeneic Stem Cell Transplantation
Authors: Jordan K. K. I. , Christensen I. J. , Heilmann C. , Sengeløv H. , Müller K. G. 
Published: December 2013

AbstractWe evaluated the prognostic role of baseline levels of C‐reactive protein (CRP) as well as CRP levels during conditioning in patients undergoing myeloablative allogeneic stem cell transplantation (SCT). Furthermore, we studied the impact of baseline clinical factors and conditioning regimens on CRP levels in the same period. We conducted a population‐based retrospective study of 349 patients undergoing SCT at the National Danish SCT centre between January 2000 and January 2009. CRP levels increased significantly during the conditioning and peaked at day −3 before infusion of the graft. Elevated CRP was associated with older age, non‐malignant disease, reduced pretransplant Karnofsky score and high‐risk leukaemia. By univariate and multivariate analyses, increased CRP levels (>10 mg/l) before the start of treatment (day −7) and at the day of graft infusion (day 0) were associated with decreased overall survival [HR 1.35 (95%CL) (1.18–1.54); P < 0.0001] and increased treatment‐related mortality [1.5 (1.24–1.82); P < 0.0001]. Similar findings were seen for mean CRP levels during the conditioning. CRP was not associated with risk of relapse or aGvHD in multivariate analysis. This study suggests that increased CRP levels before and during the conditioning are associated with baseline clinical factors and that elevated pretransplant CRP levels predict a poorer survival in SCT.
Shortcomings in the Application of Multicolour Flow Cytometry in Lymphocyte Subsets Enumeration
Authors: Njemini R. , Onyema O. O. , Renmans W. , Bautmans I. , De Waele M. , Mets T. 
Published: December 2013

AbstractThe lymphoid system is composed of numerous phenotypically distinct subsets of cells, each of which has a unique role in the effectiveness of an immune response. To distinguish specifically between these subsets, it is mandatory to detect simultaneously different cell surface antigens. This became feasible by the development of multicolour flow cytometric technologies. With these techniques, researchers now have the opportunity to study individual cells in far greater detail than previously possible. However, proper data analysis, interpretation and presentation of results will require a high level of understanding of the intricacies of the technology and the inherent limitations of the acquired data. The present report is intended to contribute to the better understanding of how the flow cytometer operates. This report may help new and inexperienced users to work appropriately with the flow cytometer.
Structural characterization of a plant photosystem I and NAD(P)H dehydrogenase supercomplex
Authors: Kouřil Roman , Strouhal Ondřej , Nosek Lukáš , Lenobel René , Chamrád Ivo , Boekema Egbert J. , Šebela Marek , Ilík Petr 
Published: December 2013

SummaryCyclic electron transport (CET) around photosystem I (PSI) plays an important role in balancing the ATP/NADPH ratio and the photoprotection of plants. The NAD(P)H dehydrogenase complex (NDH) has a key function in one of the CET pathways. Current knowledge indicates that, in order to fulfill its role in CET, the NDH complex needs to be associated with PSI; however, until now there has been no direct structural information about such a supercomplex. Here we present structural data obtained for a plant PSI–NDH supercomplex. Electron microscopy analysis revealed that in this supercomplex two copies of PSI are attached to one NDH complex. A constructed pseudo‐atomic model indicates asymmetric binding of two PSI complexes to NDH and suggests that the low‐abundant Lhca5 and Lhca6 subunits mediate the binding of one of the PSI complexes to NDH. On the basis of our structural data, we propose a model of electron transport in the PSI–NDH supercomplex in which the association of PSI to NDH seems to be important for efficient trapping of reduced ferredoxin by NDH.
Boron bridging of rhamnogalacturonan‐II, monitored by gel electrophoresis, occurs during polysaccharide synthesis and secretion but not post‐secretion
Authors: Chormova Dimitra , Messenger David J. , Fry Stephen C. 
Published: December 2013

SummaryThe cell‐wall pectic domain rhamnogalacturonan‐II (RG‐II) is cross‐linked via borate diester bridges, which influence the expansion, thickness and porosity of the wall. Previously, little was known about the mechanism or subcellular site of this cross‐linking. Using polyacrylamide gel electrophoresis (PAGE) to separate monomeric from dimeric (boron‐bridged) RG‐II, we confirmed that Pb2+ promotes H3BO3‐dependent dimerisation in vitro. H3BO3 concentrations as high as 50 mm did not prevent cross‐linking. For in‐vivo experiments, we successfully cultured ‘Paul's Scarlet’ rose (Rosa sp.) cells in boron‐free medium: their wall‐bound pectin contained monomeric RG‐II domains but no detectable dimers. Thus pectins containing RG‐II domains can be held in the wall other than via boron bridges. Re‐addition of H3BO3 to 3.3 μm triggered a gradual appearance of RG‐II dimer over 24 h but without detectable loss of existing monomers, suggesting that only newly synthesised RG‐II was amenable to boron bridging. In agreement with this, Rosa cultures whose polysaccharide biosynthetic machinery had been compromised (by carbon starvation, respiratory inhibitors, anaerobiosis, freezing or boiling) lost the ability to generate RG‐II dimers. We conclude that RG‐II normally becomes boron‐bridged during synthesis or secretion but not post‐secretion. Supporting this conclusion, exogenous [3H]RG‐II was neither dimerised in the medium nor cross‐linked to existing wall‐associated RG‐II domains when added to Rosa cultures. In conclusion, in cultured Rosa cells RG‐II domains have a brief window of opportunity for boron‐bridging intraprotoplasmically or during secretion, but secretion into the apoplast is a point of no return beyond which additional boron‐bridging does not readily occur.
Different breeding systems of three varieties ofBidens pilosainTaiwan
Authors: Huang Y‐L , Kao W‐Y 
Published: December 2013

SummaryThree varieties of the introducedBidens pilosa(Hairy beggar‐ticks;Asteraceae) co‐exist inTaiwan.Bidens pilosavar. minorand var. pilosaare naturalised, while only var. radiatais invasive. Differences in seed set in bagged versus open‐pollinated capitula, pollen–pistil interactions and the pollen/ovule (P/O) ratio were investigated among these three varieties. In contrast with the resulting 45–55% seed set in each bagged capitulum of var. minorand var. pilosa, almost no mature seeds (1–2 seeds) were found in bagged capitula of var. radiata, showing that var. radiatahas a low capability of autonomous seed production. In bagged capitula, only 1.5% of pistils of var. radiata, but 76% and 52% of those of var. minorandpilosa,respectively, had pollen tubes penetrating into their styles, suggesting that var. radiatahas strong self‐incompatibility mechanisms preventing autonomous autogamy. The P/O ratio, with mean values of 8189, 2053 and 1613 for var. radiata, var. minorand var. pilosa,respectively, were counted. These results support the hypothesis that the three varieties bear different breeding systems; the invasive var. radiatais highly self‐incompatible, whereas the other two naturalised varieties are self‐compatible.
Complete Genome of Bacillus pumilus Siphophage Blastoid
Authors: Mash Scott J. , Minahan Nicholas T. , Chamakura Karthik R. , Kuty Everett Gabriel F. 
Published: December 2013

ABSTRACT

Phage Blastoid is a siphophage that infects
Bacillus pumilus
.
B. pumilus
is widely used in agriculture but has recently been linked to cases of food poisoning. Here, we report the complete genome of Blastoid and discuss unique genomic characteristics.
Draft Genome Sequence of Deinococcus xibeiensis R13, a New Carotenoid-Producing Strain
Authors: Hu Yaochi , Xu Xian , Song Ping , Jiang Ling , Zhang Zhidong , Huang He 
Published: December 2013

ABSTRACT

Deinococcus xibeiensis
strain R13, isolated from radiation-contaminated soils, synthesizes a unique ketocarotenoid, deinoxanthin. Here, we present a 3.49-Mb assembly of its genome sequence, which can help us find the key genes of the deinoxanthin biosynthesis pathways and modify genes obtaining a high yield of the new carotenoid.
Draft Genome Sequence of Shewanella decolorationis S12, a Dye-Degrading Bacterium Isolated from a Wastewater Treatment Plant
Authors: Xu Meiying , Fang Yun , Liu Jun , Chen Xingjuan , Sun Guoping , Guo Jun , Hua Zhengshuang , Tu Qichao , Wu Liyou , Zhou Jizhong , Liu Xueduan 
Published: December 2013

ABSTRACT

Shewanella decolorationis
is a valuable microorganism for degrading diverse synthetic textile dyes. Here, we present an annotated draft genome sequence of
S. decolorationis
S12, which contains 4,219 protein-coding genes and 86 structural RNAs. This information regarding the genetic basis of this bacterium can greatly advance our understanding of the physiology of this species.
Genome Sequence of Enterobacter turicensis Strain 610/05 (LMG 23731), Isolated from Fruit Powder
Authors: Stephan Roger , Grim Christopher J. , Gopinath Gopal R. , Mammel Mark K. , Sathyamoorthy Venugopal , Trach Larisa H. , Chase Hannah R. , Fanning Séamus , Tall Ben D. 
Published: December 2013

ABSTRACT

We report the draft genome sequence of
Enterobacter turicensis
strain 610/05 (LMG 23731), isolated from fruit powder. The draft genome has a size of 4,182,790 bp and a G+C% content of 58.0.
Draft Genome Sequence of the Psychrophilic and Alkaliphilic Rhodonellum psychrophilum Strain GCM71
T
Authors: Hauptmann Aviaja L. , Glaring Mikkel A. , Hallin Peter F. , Priemé Anders , Stougaard Peter 
Published: December 2013

ABSTRACT

Rhodonellum psychrophilum
GCM71
T
, isolated from the cold and alkaline submarine ikaite columns in the Ikka Fjord in Greenland, displays optimal growth at 5 to 10°C and pH 10. Here, we report the draft genome sequence of this strain, which may provide insight into the mechanisms of adaptation to these extreme conditions.
Complete Genome Sequence of Bacillus thuringiensis Serovar Israelensis Strain HD-789
Authors: Doggett Norman A. , Stubben Chris J. , Chertkov Olga , Bruce David C. , Detter J. Chris , Johnson Shannon L. , Han Cliff S. 
Published: December 2013

ABSTRACT

Bacillus thuringiensis
is an important microbial insecticide for controlling agricultural pests. We report the finished genome sequence of
Bacillus thuringiensis
serovar israelensis strain HD-789, which contains genes encoding 7 parasporal crystals consisting of Cry4Aa3, Cry4Ba5 (2 genes), Cry10Aa3, Cry11Aa3, Cry60Ba3, and Cry60Aa3, plus 3 Cyt toxin genes and 1 hemagglutinin gene.
Extracellular Vesicles Modulate the Glioblastoma Microenvironment via a Tumor Suppression Signaling Network Directed by miR-1
Authors: Bronisz Agnieszka , Wang Yan , Nowicki Michal O. , Peruzzi Pierpaolo , Ansari Khairul I. , Ogawa Daisuke , Balaj Leonora , De Rienzo Gianluca , Mineo Marco , Nakano Ichiro , Ostrowski Michael C. , Hochberg Fred , Weissleder Ralph , Lawler Sean E. , Chiocca E. Antonio , Godlewski Jakub 
Published: December 2013

Abstract
Extracellular vesicles have emerged as important mediators of intercellular communication in cancer, including by conveying tumor-promoting microRNAs between cells, but their regulation is poorly understood. In this study, we report the findings of a comparative microRNA profiling and functional analysis in human glioblastoma that identifies miR-1 as an orchestrator of extracellular vesicle function and glioblastoma growth and invasion. Ectopic expression of miR-1 in glioblastoma cells blocked in vivo growth, neovascularization, and invasiveness. These effects were associated with a role for miR-1 in intercellular communication in the microenvironment mediated by extracellular vesicles released by cancer stem–like glioblastoma cells. An extracellular vesicle–dependent phenotype defined by glioblastoma invasion, neurosphere growth, and endothelial tube formation was mitigated by loading miR-1 into glioblastoma-derived extracellular vesicles. Protein cargo in extracellular vesicles was characterized to learn how miR-1 directed extracellular vesicle function. The mRNA encoding Annexin A2 (ANXA2), one of the most abundant proteins in glioblastoma-derived extracellular vesicles, was found to be a direct target of miR-1 control. In addition, extracellular vesicle–derived miR-1 along with other ANXA2 extracellular vesicle networking partners targeted multiple pro-oncogenic signals in cells within the glioblastoma microenvironment. Together, our results showed how extracellular vesicle signaling promotes the malignant character of glioblastoma and how ectopic expression of miR-1 can mitigate this character, with possible implications for how to develop a unique miRNA-based therapy for glioblastoma management. Cancer Res; 74(3); 738–50. ©2013 AACR.