bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and Engineering of Novel N‐Methyltransferases
Authors: Heinz Florian , Meinert Hannes , Somvilla Ina , Menke Marian J. , Dörr Mark , Bayer Thomas , Bornscheuer Uwe 
Published: February 2025

The methylation of small molecules is of immense value to various chemical industries. Since chemical alkylation requires rather unselective and harsh reaction conditions, sustainable biocatalytic approaches are highly desired. Methyltransferases, particularly N‑methyltransferases (NMTs), facilitate the selective introduction of methyl (and other alkyl) groups under mild conditions in aqueous solution and are a promising alternative to chemical synthesis. In this work, we expanded the toolbox of NMTs through the bioinformatic‑assisted mining of databases and the high‐throughput characterization of novel NMTs by a fluorescence‐based assay. The latter can assess enzyme activity in cell lysates, independent of the investigated substrate, and was used to guide the engineering of newly identified NMTs. Mutants, exhibiting up to 8‐fold improved selective methylation of (non‐)natural N‑heterocyclic drug precursors, were identified and promise great potential for synthetic applications.
Effect of carrier volume and nozzle type on spray deposition within the canopy, disease control and yield in peanut
Authors: Sapkota Madan , Virk Simerjeet S. , Kemerait Robert C. , Prostko Eric P. , Butts Thomas R. 
Published: February 2025

AbstractEffective disease and pest management in peanut (Arachis hypogea L.) requires adequate spray penetration within the canopy during pesticide applications. Field studies were conducted to assess spray deposition within the peanut canopy at three carrier volumes of 10, 15 and 20 gallons per acre (GPA), with each volume applied using three different nozzle types (extended range [XRC], air induction extended range [AIXR], and Turbo TeeJet Induction [TTI]). Spray deposition was assessed using water at various application timings (45, 60, 90, and 120 DAP) by placing water‐sensitive paper at upper, middle, and lower positions within the peanut canopy. Fungicide applications using different carrier volume and nozzle treatments were made at regular intervals throughout the season, and disease ratings along with peanut yield were recorded at harvest. The carrier volume of 20 GPA consistently provided the greatest deposition in the upper and middle canopy, followed by 15 and 10 GPA. The XRC nozzle exhibited the greatest deposition in the upper canopy, followed by the AIXR and TTI nozzles. Within the lower canopy, the effect of carrier volume and nozzle type on spray deposition varied among the application timings. For disease control, the lower carrier volume of 10 GPA and XRC nozzle showed an increased incidence of late leaf spot (Nothopassalora personata) and southern stem rot (Sclerotium rolfsii Sacc.) in one of the study years. Carrier volume and nozzle type did not affect peanut yield during both years. Overall, the findings suggest that spray deposition within the peanut canopy is influenced by carrier volume and nozzle type; however, it does not necessarily lead to reduced peanut yield, especially in most fields with low to moderate disease pressure.
Difference and Influencing Factors of the City Logistics Development Level in China
Authors: Zhang Man , Kou Zheng 
Published: February 2025

ABSTRACTUrban logistics is an important bridge connecting urban supply and demand, and its development level is crucial to urban economic development. To explore the development level, spatial and temporal differences, and influencing factors of urban logistics in China, this article uses the entropy weight method, Theil index, and hierarchical regression model to analyze the panel data of urban logistics in 31 provinces (autonomous regions and municipalities) in China. The research conclusion provides a reference for the government to formulate a balanced development policy for urban logistics. It has important practical significance for guiding logistics enterprises to adjust their regional distribution.
B‐Corp Certification: Systematic Literature Review and Research Agenda
Authors: Attanasio Giovanna , Battistella Cinzia , Chizzolini Elia 
Published: February 2025

ABSTRACTThis paper aims to study the adoption and impact of B‐Corp certification by systematically reviewing key papers on this topic published in peer‐reviewed journals from 2006 to 2024. The paper proposes a conceptual framework that provides a comprehensive overview of research themes, subdivided into five broad research focuses: drivers and enablers for organisations seeking certification, barriers they encounter, implementation issues of B‐Corp certification and the outcomes achieved post‐certification. The study offers a thorough overview of the academic literature on B‐Corp certification, laying a strong foundation for future research on the subject. The literature reveals similarities in objectives, approaches and methods, often yielding overly optimistic results that fail to capture the complex impact of B‐Corp certification. A range of research opportunities is presented, establishing a research agenda to guide future studies in the field. The findings can support decision‐making regarding the adoption and renewal of certification by evaluating its cost–benefit. Policymakers can utilise this comprehensive overview of knowledge to inform their decisions on regulatory actions.
Building Resource Capabilities Through Green Servitization and ISO 14001 for Sustainable Performance: Perspectives From Manufacturing Firms
Authors: Oyelakin Idris Oyewale , Ting Ding Hooi , Yusuf Asnidar Hanim , Arbak Salwani , Dhar Bablu Kumar 
Published: February 2025

ABSTRACTManufacturing firms are increasingly adopting green servitization and ISO 14001 to enhance resource capabilities and achieve sustainable performance amid escalating challenges of resource depletion and waste management. This study explicitly links these practices to broader CSR principles, focusing on stakeholder engagement and accountability to drive environmental and social sustainability. Specifically, it examines the interplay between green servitization and ISO 14001 in fostering sustainable performance within manufacturing firms. The findings reveal the critical mediating role of ISO 14001 in translating green servitization initiatives into tangible sustainability outcomes while building stakeholder trust. Practical contributions emphasize fostering green innovation, integrating biodegradable materials, and leveraging green digital technologies to enhance resilience and operational efficiency. The research also advances CSR‐related theoretical contributions by framing green servitization and ISO 14001 as strategic resources for achieving Sustainable Development Goals.
Fox on the Run—Cheaper Camera Traps Fail to Detect Fast‐Moving Mesopredators
Authors: McHenry R. , Mitchell L. J. , Marshall C. , Smart J. , de Raad A. L. , Andersen R. 
Published: February 2025

ABSTRACTCamera trapping for detecting wildlife is increasingly used as a primary method of non‐invasive wildlife monitoring. Yet understanding among researchers and conservationists on how camera trap make, and model affect detection rates is limited. Published studies often fail to make clear why a given camera trap model was chosen or what specifications or parameters were used to capture target species within a given study area, prohibiting replicability. Here we present a comparison of predator and herbivore detection efficacy using three makes and models of camera trap at differing price ranges, year of release (hereafter vintages) and specifications. We used a passive monitoring survey design at six sites in open field conditions across the Flow Country, Northern Scotland. Detection efficacy varied substantially between grades and vintages of camera traps and depended on species captured. Older models of camera with lower trigger speed and night vision range performed particularly poorly for nocturnal predatory mammal detection. This has implications for how researchers, conservationists, developers and other users approach experimental design and analyses, but also on the conclusions that may be drawn from studies. We caution against using the results of one or more camera trap studies using different makes and models of cameras to inform experimental design or policy interventions.
An Established Plant Invader May Still Benefit From Increasing Genetic Diversity—Insights From Artificial Populations in a Common Garden Experiment
Authors: Watermann L. Y. , Durka W. , Erfmeier A. 
Published: February 2025

ABSTRACTGenetic diversity and competitive ability, though extensively studied in the context of biological invasions, are still poorly understood in their relative importance, especially when shifting the perspective from an individual plant's phenotype to overall population performance. Most approaches addressing the role of genetic diversity involve the comparison of standing genetic variation in field populations combined with experimental treatments on individual plants. Composing predefined mixtures of populations to manipulate genetic diversity would be an experimental approach to test for direct effects on population performance. We determined pairwise genetic distances among 16 invasive and 22 native populations of Jacobaea vulgaris GAERTN. using single nucleotide polymorphisms (SNP). Based on this information, we created each 15 de‐novo populations with different levels of genetic diversity for both origins. These de‐novo populations were subjected to three levels of decreasing microsite availability by using a matrix of either 0, 5, or 10 individuals of Festuca rubra. We monitored population performance continuously throughout two growing seasons to study effects of interactions between origin, microsite availability, and genetic diversity with (generalized) linear effects models. This allowed us to uncover whether the relative importance of those factors varies with the life‐stage of this biennial species. We found no ambiguous patterns on the hypothesized beneficial effect of genetic diversity for J. vulgaris populations. Native populations tended to respond negatively to increasing genetic diversity, especially under more favorable site conditions, but this was not a persistent pattern and was only evident through continuous monitoring. Invasive populations could benefit from increasing genetic diversity during early establishment, but not in interaction with restricted microsite availability. Our results do suggest that genetic variation supports population establishment and performance under certain environmental conditions. Therefore, for recommendations in nature conservation, efforts should still aim at limiting propagule addition in already invaded areas, even in well‐established invasive species.
Enhancing the performance of molybdenum disulfide‐based solar water evaporation systems by tuning the synthesis temperature
Authors: Hasmin Hudya Fitra , Djajasuminta Livia Ilona , Dwiputra Muhammad Adam , Emrinaldi Tengku , Roza Liszulfah , Umar Akrajas Ali , Ginting Titian Riski , Fauzia Vivi 
Published: February 2025

AbstractMoS2 has been developed for use in solar water evaporation systems. Studies have shown that the temperature and duration of synthesis significantly affect the phase, number of layer, and particle size of MoS2. However, there is a lack of comprehensive research on how these factors, including the wettability of MoS2, influence the water evaporation rate. In this study, we synthesized MoS2 at two distinct temperatures: 180 and 200°C using a hydrothermal method. This study shows MoS2 synthesized at 180°C forms smaller 2H‐MoS2 nanosheets with small grains size and more defects and exhibits higher evaporation rates kg m−2 h−1. This increased evaporation is due to smaller nanosheets provides a larger surface area, enhancing light absorption and thermal energy conversion. The defective sites in MoS2, especially along its edges, act as preferential adsorption sites for water molecules. This facilitates water diffusion, and consequently increases wettability and accelerates evaporation. The use of MoS2 on air‐laid paper as photothermal materials also demonstrated excellent salt rejection (>99%). This work demonstrates the novelty of tuning the efficiency of MoS2‐based solar water evaporation systems by simply adjusting the synthesis temperature. This approach is an innovative method for industrial‐scale solar evaporation applications.
Any neonatal morbidityAdverse perinatal outcomes of unvaccinated pregnant women with respiratory symptoms during the COVID‐19 pandemic: Evidence from a Brazilian multicenter study
Authors: Da‐Costa‐Santos Juliana , dos Reis Junior Paulo Sergio , Luz Adriana Gomes , Cecatti Jose Guilherme , Souza Renato T. , Tedesco Ricardo P. , Fernandes Karayna G. , Martins‐Costa Sérgio H. A. , Peret Frederico J. A. , Feitosa Francisco E. , Traina Evelyn , Cunha Filho Edson V. , Vettorazzi Janete , Haddad Samira M. , Andreucci Carla B. , Guida José P. , Correa Junior Mario D. , Dias Marcos A. B. , Oliveira Leandro G. , Melo Junior Elias F. , Da Luz Marília G. Q. , Costa Maria Laura ,   for the REBRACO Study Group
Published: February 2025

AbstractObjectiveThe current study aimed to describe risk factors for adverse perinatal outcomes (APOs) among pregnant women nonvaccinated for COVID‐19 who had respiratory symptoms.MethodsA nested case–control study was performed within the REBRACO (in Portuguese, the Brazilian Network of COVID‐19 During Pregnancy) initiative. Women were recruited during pregnancy in 15 maternity hospitals in Brazil from February 1, 2020, to February 28, 2021, while seeking medical care for respiratory symptoms, and were followed up until childbirth regardless of confirmation of COVID‐19. For this analysis, women were divided into two groups: (1) those with APOs, defined as the occurrence of fetal or neonatal death, preterm delivery, 5‐min Apgar score <7, neonatal respiratory distress, neonatal mechanical ventilation, admission to the neonatal intensive care unit, small‐for‐gestational‐age newborn, or any neonatal morbidity; and (2) those without APOs.ResultsThe total number of women included in this analysis was 481, with 210 having APOs (43.7%). The characteristics independently associated with APOs were a composite outcome of severe acute respiratory syndrome, maternal admission at the intensive care unit, or maternal death (relative risk [RR], 3.30 [95% confidence interval (CI), 1.38–7.89]), living in the North and Northeastern regions of Brazil (RR, 3.09 [95% CI, 1.13–8.41]), and pre‐eclampsia (RR, 2.77 [95% CI, 1.19–6.43]).ConclusionSevere maternal illness was strongly associated with APO regardless of COVID‐19 confirmation. It is essential to provide sufficient and timely health care for women who have respiratory symptoms compatible with COVID‐19.
MMCAF: A Survival Status Prediction Method Based on Cross‐Attention Fusion of Multimodal Colorectal Cancer Data
Authors: Tan Xueping , Wu Dinghui , Wang Hao , Zhao Zihao , Ge Yuxi , Hu Shudong 
Published: February 2025

ABSTRACTThe employment of artificial intelligence methods in computer‐assisted diagnosis systems is critical for colorectal cancer survival analysis and prognosis. However, due to the low prediction accuracy of single‐modal data research and the complexity of multimodal data fusion methods, the current study's effect on colorectal cancer is minimal. To address this issue, the authors offer a multimodal cross attention fusion (MMCAF) technique for predicting colorectal cancer survival status. First, feature engineering is used to create feature sets for every mode and to address the heterogeneity of multimodal data. Second, a three‐mode fusion technique is used to allocate weight to single‐mode and multimodal features via channels and cross‐attention processes. Lastly, the cross‐entropy loss function is minimized in order to estimate the classification survival. The experimental results reveal that the MMCAF approach predicts survival states with 97.73% accuracy and an area under the receiver operating characteristic curve (AUC) of 0.99. When compared to the best outcome of other fusion algorithms (feature concatenation), the prediction accuracy increases by about 6 percentage points, while the AUC increases by 7 percentage points. This finding thoroughly demonstrates MMCAF's efficacy in predicting colorectal cancer survival.
Modelling the Spatio‐Temporal Effects of Water Level Control in an Agricultural Collector Ditch Network
Authors: Paavonen Elina , Salo Heidi , Salla Aleksi , Koivusalo Harri 
Published: February 2025

ABSTRACTThe study focused on modelling open channel flow in an agricultural ditch network. The objectives were (1) to set up a computational tool to explore the hydraulic impacts of water level control in a collector ditch network and (2) to test the effects of damming height and ditch flow resistance on the water level and its relation to field drainage. The model platform FLUSH is a hydrological model that couples field hydrology with a description of open channel flow network hydraulics under subarctic climate conditions. A one‐dimensional model setup described an existing collector ditch network in North Ostrobothnia, Finland, where fields under normal farming practices were instrumented to explore agricultural water management designs. In the model, drainage was fed to the ditch network as a spatially varied inflow. Four weir heights and flow resistances were used to run 16 simulations. Although flow resistance increased water levels in the ditch network, the absolute damming effect was the highest with low flow resistance. The water level control decreased the flow velocity within the control, which increased to 20%. According to the model simulations, a single weir downstream affected field drainage in up to 25% of the field area. The model provides a transparent means to investigate water management methods in fields and in collector ditches and to support the design of catchment‐scale water level control structures.
Are Neurocognitive Deficits Associated With Gastrointestinal Symptoms in Children due to COVID‐19?
Authors: Megari Kalliopi , Thomaidou Evanthia , Palioura Maria Eleni , Parasxiakos Stelios , Skoutara Aglaia , Stougioude Panagiota , Theodoratou Maria , Sofologi Maria , Efthymiou Efthymia , Papadopoulou Soultana , Katsarou Dimitra V. 
Published: February 2025

ABSTRACTBackground and ObjectivesChildren with gastrointestinal symptoms due to COVID‐19 may experience neurocognitive deficits. Symptoms include difficulty in concentration, memory and other cognitive functions. The deficits could be caused by the virus itself or due to the physical and psychological stress of the pandemic.Materials and MethodsIn this study, we examined 65 children, 32 children with gastrointestinal symptoms, and we compared them to 33 children without gastrointestinal symptoms due to COVID‐19, to investigate whether gastrointestinal symptoms affected neurocognitive status in children with COVID‐19.ResultsWe found that there was a significant relationship between cognitive function and GI symptoms, as well as between cognitive function and the severity of these symptoms. This indicates that COVID‐19 patients with GI symptoms may be at risk for developing problems with their memory and other aspects of cognition.ConclusionsEarly detection and intervention may help young children recover faster and return to their typical cognitive functioning.
Zn(II) Affinity and Structural Conformations of 2His‐2Cys Zinc Finger‐Like Motif Peptide Determined by Ion Mobility–Mass Spectrometry and PM6 Molecular Modeling
Authors: Adomako Richmond A. , Owusu Michael B. , Oberdick Rebekah K. , Senyah Kwabena , Asare Perfect , Spezia Riccardo , Angel Laurence A. 
Published: February 2025

ABSTRACTThis study focuses on investigating the conformational structure and zinc(II) affinity of a zinc finger‐like motif (ZFM) peptide with the sequence acetyl‐His1‐Cys2‐Gly3‐Pro4‐Gly5‐His6‐Cys7, where bold highlights the potential zinc(II) binding sites. Zinc fingers are crucial protein motifs known for their high specificity and affinity for zinc ions. The ZFM peptide's sequence contains the 2His‐2Cys zinc‐binding sites similar to those in natural zinc finger proteins but without the hydrophobic core, making it a valuable model for studying zinc(II)–peptide interactions. Previous research on related peptides showed that collision cross sections and B3LYP modeling predicted that the His‐2Cys‐carboxyl terminus coordination of zinc(II) was more stable than the 2His‐2Cys. Employing a comprehensive approach integrating ion mobility–mass spectrometry and theoretical modeling techniques, various zinc(II) binding modes of the ZFM have been thoroughly compared to ascertain their influence on the competitive threshold collision‐induced dissociation method for measuring the relative gas‐phase Zn(II) affinity of the ZFM peptide. The measured Zn(II) affinity of ZFM is greater than those measured recently for two peptides with similar primary structures, acetyl‐His1‐Cys2‐Gly3‐Pro4‐Gly5‐Gly6‐Cys7 and acetyl‐Asp1‐His2‐Gly3‐Pro4‐Gly5‐Gly6‐Cys7, indicating the preference for the His1‐Cys2‐His6‐Cys7 side groups for coordinating zinc(II) over the His‐2Cys‐carboxyl terminus or Asp‐His‐Cys‐carboxyl terminus in these related heptapeptides.
Predictors of Adherence to Diabetes Self‐Care Management Among Diabetes Patients in Public Health Facilities of Central Ethiopia: A Multicenter Cross‐Sectional Study
Authors: Lonsako Arega Abebe , Samuel Bereket , Ayele Abdurehman , Kasse Tsehaynew , Haile Addisalem 
Published: February 2025

ABSTRACTBackgroundDiabetes mellitus is a global public health challenge. Adherence to self‐care management is critical to reducing complications. Evidence on adherence levels and influencing factors among diabetes patients in central Ethiopia is scarce. This study assessed adherence levels and predictors among diabetes patients in Kembata Zone, Central Ethiopia.MethodsA multicenter cross‐sectional study was conducted from May 1 to 30, 2024, involving 414 diabetes patients. Data were collected via interviewer‐administered questionnaires and analyzed using Statistical Package for Social Science (SPSS) version 26. Systematic random sampling was employed, and multivariable logistic regression identified adherence predictors. Adjusted odds ratios (AORs) with 95% confidence intervals (CIs) were calculated, with statistical significance set at p < 0.05.ResultsOverall good adherence to diabetes self‐care was 24.4% (95% CI: 20.3–28.5). Adherence by domain was 35.5% for dietary management, 20.5% for exercise, 42.3% for hypoglycemia management, 24.9% for insulin administration, and 29.7% for blood glucose testing. Urban residence (AOR = 1.70, 95% CI: 1.02–2.84) and diabetes duration of 1–5 years (AOR = 2.99, 95% CI: 1.39–6.48) positively predicted adherence, whereas primary education was negatively associated (AOR = 0.33, 95% CI: 0.15–0.74).ConclusionsAdherence to diabetes self‐care management in the study area was low, particularly in exercise and insulin administration. Urban residence and shorter diabetes duration were positive predictors, whereas lower education hindered adherence. Urgent health education tailored to rural and less‐educated populations is needed, emphasizing exercise and insulin management to improve outcomes.