bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peran Zakat Dalam Meningkatkan SDM Santri Di Yayasan Al-Kamilah
Authors: Sa'diyah Khotimatus , Zami R. Mohd Zam , AN Wiwik Hasbiyah 
Published: February 2025

Pengabdian kepada masyarakat bermula atas permasalahan kondisi masyarakat khususnya pada anak-anak yatim dan dhu’afa dalam kondisi yang sangat memprihatinkan karena keterbatasan ekonomi dan sebagian besar mereka banyak yang tidak sekolah atau putus sekolah. Hal ini menyebabkan rendahnya kualitas sumber daya manusia. Al Kamilah merupakan salah satu yayasan kesejahteraan sosial dimana pemberdayaan SDM santri sangatlah diperlukan guna mewujudkan kemandirian individu agar nantinya anak-anak yatim dan dhua’fa dapat meningkatkan kesejahteraannya. Pelaksanaan pengabdian kepada masyarakat ini berfokus pada Peran Zakat Dalam Meningkatkan SDM Santri di Yayasan Al-Kamilah. Metode Pelaksanan pengabdian masyarakat ini adalah melalui penyuluhan dan pendampingan melalui rangkaian kegiatan identifikasi, pengkajian serta proses terencana dengan 3 tahapan yaitu 1) Pengumpulan data, dengan memberikan data wawancara kepada santriwan santriwati tentang keluhan dan kelemahan dalam pengelolaan dana zakat 2). Penyuluhan pendayagunaan dan pengelolaan zakat 3). Pelatihan tentang implementasi pendayagunaan zakat pada santriwan santriwati berdasarkan UU No 23 tahun 2011. Untuk mendukung hal itu diperlukan sebuah rencana kegiatan melalui tahap persiapan dengan studi literatur, persiapan PKM, dan melakukan koordinasi dengan instansi lembaga yayasan. Tahap penentuan lokasi dengan melakukan kunjungan ke lokasi. Dan tahap pelaksanaan melalui implementasi penyuluhan dan sosialisasi. Hasil Pengabdian masyarakat yang bertempat di Yayasan Al-Kamilah adalah melalui pengelolaan dan pemberdayaan zakat yang professional diharapkan dapat meningkatkan kualitas SDM santri di Yayasan Al Kamilah guna meningkatkan kesejahteraannya baik secara ekonomi maupun sosial.
The Overriding of Product Innovation, Aesthetic Value, and Social Media Marketing to Increase Consumer Purchase Intention of ‘Malangan’ Batik
Authors: Aliyyuldafa Natasya Citra , Anjaningrum Widiya Dewi 
Published: February 2025

Competition among businesses is becoming increasingly intense to create products that can increase consumer purchase intention, especially in the same industry. This includes what happens to ‘Malangan’ batik products, where challenges must be faced not only with local products but also with products from other countries. This study intends to determine the influence of product innovation, social media marketing, and aesthetic value on customers' purchase intentions toward ‘Malangan’ batik. The study population comprises all ‘Malangan’ batik consumers in Malang city, with an undetermined number of individuals. The accidental sampling technique was used to select respondents and gather data for this study by distributing questionnaires to the final sample size of 180 people. This research uses quantitative methods analyzed with SmartPLS-4.1.0.8. The results of this study found that continuous product innovation, effective implementation of social media marketing, and a strong emphasis on aesthetic value in products are essential strategies for businesses to significantly increase consumer purchase intention. Future studies should consider expanding the scope of the research to include additional variables that may influence consumer purchase intentions regarding ‘Malangan’ batik.
Metal-dependent cell death resistance contribute to lymph node metastasis of oral squamous cell carcinoma
Authors: Liu Xuan-Hao , Wang Guang-Rui , Zhong Nian-Nian , Zhu Zheng-Rui , Xiao Yao , Li Zheng , Bu Lin-Lin , Liu Bing 
Published: February 2025

ObjectivesFerroptosis and cuproptosis can be summarized as metal-dependent cell death. This study aimed to explore the expression of metal-dependent cell death resistance (MCDR) characteristics in tumor cells of oral squamous cell carcinoma (OSCC) and to explore its relationship with lymph node metastasis (LNM).MethodsBy integrating single-cell data of OSCC from public databases, an expression matrix comprising 127,149 cells was constructed. Gene set scores were calculated using the irGSEA package, and GO and KEGG analyses were performed to identify enriched pathways. The R package monocle3 was employed to calculate the cell trajectory and infer evolutionary patterns. The MuSiC2 package was employed to enable the evaluation of cell proportions. Cell-cell interaction information was analyzed using the CellChat package. The expression of cathepsin V (CTSV), glutathione peroxidase 4 (GPX4), and cyclin-dependent kinase inhibitor 2A (CDKN2A) was validated via immunohistochemistry and multiplex immunohistochemistry in oral mucosa (OM), non-metastatic primary tumors (nPT), and metastatic primary tumors (mPT). Additionally, R package oncoPredict was utilized to identify potential drug sensitivities.ResultsThe malignant cells in OSCC were divided into five subtypes, among which Epi_2 existed more in mPT and had higher MCDR characteristics. In addition, Epi_2 enriched multiple malignant-related pathways such as HEDGEHOG, NOTCH, and MYC. The spatial transcriptome and bulk RNA data verified that the proportion of Epi_2 in mPT was higher than that in nPT. Cell communication analysis showed that the effect of Epi_2 on endothelial cells was enhanced, which was mainly reflected in VEGFR and CXCL signaling pathways. Immunohistochemical results showed that the expression of Epi_2 characteristic markers CTSV and GPX4 in mPT was significantly higher than that in nPT. Multiplex immunohistochemical results showed that the co-expression cells of CTSV, GPX4 and CDKN2A in mPT were more than those in nPT. OSCC patients with high Epi_2 characteristics may have immunotherapy resistance and anti-EGFR treatment resistance. Doramapimod was identified as a sensitive drug.ConclusionThere is a type of malignant cells with characteristics of MDCR in OSCC, which is related to LNM and treatment resistance. It provides a predictive marker for early diagnosis of LNM.
The results of polymerase chain reaction and MALDI-TOF mass spectrometry versus phenotypic distinction between Klebsiella pneumoniae and Klebsiella oxytoca
Authors: Palusiak Agata , Maciejewska Anna , Łukasiewicz Jolanta 
Published: February 2025

IntroductionKlebsiella pneumoniae and K. oxytoca are members of Enterobacteriaceae. They are Gram-negative, non-motile rods that are ubiquitous in the environment and part of the human intestinal microbiota. These opportunistic pathogens may cause pneumonia and urinary tract infections. Klebsiella species are genetically and biochemically similar; therefore, it is important to find reliable methods for their differentiation.MethodsThis study presents the results of biochemical assays, PCR, and MALDI-TOF mass spectrometry (MS) performed on 35 Klebsiella isolates obtained from the urine of patients from central Poland.ResultsAmong biochemical methods, the indole test demonstrated the highest discriminatory power, whereas the determination of growth at 10°C was the least effective. For all strains biochemically identified as K. pneumoniae, a 108-bp amplicon was detected, indicating the presence of the rpoB gene in their genome. Only 12 K. oxytoca isolates produced a product of the pehX gene. All tested strains were analyzed using the MALDI-TOF Biotyper, which confirmed, with high-quality scores, their identification based on api 20E and indole tests. Strain 0.011 was identified as Raoultella ornithinolytica.ConclusionMALDI-TOF MS analysis proved to be the most reliable method for identifying K. oxytoca and K. pneumoniae, with the potential for phylogroup differentiation.
Loss of Bmal1 impairs the glutamatergic light input to the SCN in mice
Authors: Korkmaz Hüseyin , Anstötz Max , Wellinghof Tim , Fazari Benedetta , Hallenberger Angelika , Bergmann Ann Kathrin , Niggetiedt Elena , Güven Fatma Delâl , Tundo-Lavalle Federica , Purath Fathima Faiba A. , Bochinsky Kevin , Gremer Lothar , Willbold Dieter , von Gall Charlotte , Ali Amira A. H. 
Published: February 2025

IntroductionGlutamate represents the dominant neurotransmitter that conveys the light information to the brain, including the suprachiasmatic nucleus (SCN), the central pacemaker for the circadian system. The neuronal and astrocytic glutamate transporters are crucial for maintaining efficient glutamatergic signaling. In the SCN, glutamatergic nerve terminals from the retina terminate on vasoactive intestinal polypeptide (VIP) neurons, which are essential for circadian functions. To date, little is known about the role of the core circadian clock gene, Bmal1, in glutamatergic neurotransmission of light signal to various brain regions.MethodsThe aim of this study was to further elucidate the role of Bmal1 in glutamatergic neurotransmission from the retina to the SCN. We therefore examined the spontaneous rhythmic locomotor activity, neuronal and glial glutamate transporters, as well as the ultrastructure of the synapse between the retinal ganglion cells (RGCs) and the SCN in adult male Bmal1−/− mice.ResultsWe found that the deletion of Bmal1 affects the light-mediated behavior in mice, decreases the retinal thickness and affects the vesicular glutamate transporters (vGLUT1, 2) in the retina. Within the SCN, the immunoreaction of vGLUT1, 2, glial glutamate transporters (GLAST) and VIP was decreased while the glutamate concentration was elevated. At the ultrastructure level, the presynaptic terminals were enlarged and the distance between the synaptic vesicles and the synaptic cleft was increased, indicative of a decrease in the readily releasable pool at the excitatory synapses in Bmal1−/−.ConclusionOur data suggests that Bmal1 deletion affects the glutamate transmission in the retina and the SCN and affects the behavioral responses to light.
Clinical application of repetitive transcranial magnetic stimulation in the treatment of chronic pelvic pain syndrome: a scoping review
Authors: Luo Chunmei , Zhang Baocheng , Zhou Jing , Yu Keqiang , Chang Degui 
Published: February 2025

IntroductionChronic pelvic pain syndrome is a common condition characterized by persistent symptoms that are difficult to treat. Repetitive transcranial magnetic stimulation (rTMS) is considered a safe treatment option for alleviating chronic pelvic pain, but different stimulation protocols can affect pain relief outcomes. Establishing an optimal stimulation protocol can enhance the uniformity and consistency of rTMS to provide a potentially effective therapeutic intervention. This review sought to systematically review and assess the existing literature on transcranial magnetic stimulation in patients experiencing chronic pelvic pain syndrome, evaluate the therapeutic efficacy, and determine the most effective stimulation protocol.MethodsA comprehensive search was conducted across three databases, supplemented by manual searches. Two researchers independently reviewed and extracted relevant studies and subsequently performed a thorough analysis of all available clinical data.ResultsA total of eight studies were ultimately incorporated into the analysis. These comprised two randomized controlled trials, one self-controlled trial, two case reports, and three prospective studies. All studies demonstrated a notable reduction in pain scores post-treatment.ConclusionrTMS has demonstrated efficacy in alleviating pain in individuals suffering from chronic pelvic pain syndrome. It is regarded as a safe intervention with minimal adverse effects. Nonetheless, the variability observed across studies hindered our ability to conclusively determine the most effective stimulation sites and parameters. Additional research is essential to reduce bias, enhance methodological rigor, and ascertain the optimal conditions and indications for brain stimulation to optimize the therapeutic effectiveness of rTMS.Systematic Review Registrationhttps://inplasy.com/projects/, identifier INPLASY2023120112.
Computer-assisted stereoelectroencephalography planning: center-specific priors enhance planning
Authors: Dasgupta Debayan , Elliott Cameron A. , O’Keeffe Aidan G. , Rodionov Roman , Li Kuo , Vakharia Vejay N. , Mirza Farhan A. , Tahir M. Zubair , Tisdall Martin M. , Miserocchi Anna , McEvoy Andrew W. , Ourselin Sebastien , Sparks Rachel E. , Duncan John S. 
Published: February 2025

ObjectivesThis study aims to refine computer-assisted planning (CAP) of SEEG implantations by adding spatial constraints from prior SEEG trajectories (“Priors”) to improve safety and reduce manual adjustments, without increasing planning time.MethodsRetrospective validation based on 159 previously implanted trajectories (11 cases) planned by the clinical standard CAP and CAP constrained with spatial priors (“CAP + Priors”). Constraints included 31 target and 51 entry zones, created from 98 consecutive patients (763 implanted SEEG trajectories). Each of the 159 previously implanted trajectories was planned by two fellows, once with CAP and once with CAP + Priors, in a randomized order. The time taken to generate the initial computer-generated plan (T1) and the user-edited final plan (T2) were recorded together with the proportions of electrodes that required subsequent adjustments. Clinical implantability was assessed via a blinded review of each trajectory by five independent epilepsy neurosurgeons with expertise in SEEG implantation.ResultsExpert raters considered 88.5% of trajectories implantable, with no difference in acceptability between CAP alone and CAP + Priors (p = 0.79). Median (IQR) T1 for CAP to produce complete automated implantation was 4.6 (0.85) min vs. CAP + Priors was 6.3 (2.6) min (p = 0.03). There was no significant difference in T2 (time to complete surgeon-edited plan): CAP median (IQR) 105 (22) min, and CAP + Priors median (IQR) 96 (68) min (p = 0.92). The CAP + Priors risk score was significantly lower than that for the previously actually implanted trajectories for the 11 plans analyzed (p = 0.004), and no different from CAP alone planning. A significant reduction was observed in manual adjustments required with CAP + Priors in the cingulate gyrus.ConclusionUsing spatial priors from previous implantations enhances SEEG CAP and increases the granularity of trajectory planning. This approach facilitates more standardized planning and allows for the incorporation of experience from multiple expert centers, decreasing the risk of the resultant trajectories and reducing the proportion of trajectories that require manual planning without significantly increasing planning time.
Assessment of the impact of reconstitution therapies—cladribine tablets and alemtuzumab—on the atrophy progression among patients with relapse-remitting multiple sclerosis
Authors: Pogoda-Wesołowska Aleksandra , Stachura Ignacy , Zegadło Arkadiusz , Maciągowska-Terela Marzena , Sobolewska Karolina , Dębiec Aleksander , Staszewski Jacek , Stępień Adam 
Published: February 2025

IntroductionImmune reconstitution therapies (IRT) are highly effective therapies for multiple sclerosis (MS). Among IRT, we can distinguish partially selective therapies such as cladribine in tablets (CLAD) and non-selective therapies, which include alemtuzumab (ALEM). Today, it is known that these therapies are effective in controlling the relapse activity of the disease and the progression of clinical disability, which has been proven both in clinical trials and in real world evidence (RWE). However, there is a lack of data assessing the effect of IRT on the neurodegenerative process, which is intensified in patients with MS. The aim of the study was to assess the effect of IRT treatment on the degree and pattern of brain atrophy in patients with MS during 3 years of observation.MethodsPatients with relapsing-remitting MS (RRMS) treated with CLAD and ALEM were retrospectively recruited for the study. Demographic, clinical, and magnetic resonance imaging (MRI) data were collected at 4 time points: before the treatment and one, two, and three years after the treatment. MRI examinations were analyzed volumetrically using Freesurfer software. Global and regional changes in atrophy were assessed by calculating percentage changes in volume between time points. Results of drug groups were compared with each other.ResultsAfter 3 years of follow-up, statistically significant differences between groups were observed in hippocampus [p &lt; 0.01] and amygdala volume changes [p &lt; 0.01]. Ventral diencephalon atrophy was noted in both groups. On the other hand, in both groups, no significant atrophy of white and grey matter was noted. In addition, an increase in the thalamus volume was observed.DiscussionIn the studied groups, IRT therapies were shown to slow down the atrophy process in MS patients to a similar extent. These therapies may play a neuroprotective role by increasing the volume of the thalamus and hippocampus. The study was limited by the small number of both groups. Therefore, further studies are needed to fully assess the effect of reconstitution therapies on neurodegenerative processes in patients with RRMS.
Tumor gene expression signatures associated with outcome in large B−cell lymphoma treated with CD19-directed CAR T−cell therapy (axicabtagene ciloleucel)
Authors: Tian Yuan , Budka Justin , Locke Frederick L. , Westin Jason R. , To Christina , Tiwari Gayatri , Mao Daqin , Bedognetti Davide , Shen Rhine R. , Andrade Jorge , Filosto Simone 
Published: February 2025

IntroductionCAR T cell therapy provided transformative outcomes for patients with B-cell lymphoma; however, a large fraction of patients remains at risk for relapse, underlying the need to uncover mechanisms of resistance and predictive biomarkers. Herein, we leveraged the ZUMA-7 phase III randomized trial of relapsed/refractory large B-cell lymphoma (LBCL) patients treated with axicabtagene ciloleucel (axi-cel; CD19-targeting CAR T cells) to discover tumor gene expression signatures (GES) associated with outcome.MethodsWith tumor transcriptomics from 134 axi-cel patients, we employed multivariate penalized Cox models analyzing event-free survival (EFS), progression-free survival (PFS), and duration of response (DOR).Results and DiscussionWe identified two novel GES, a six-gene/transcript signature (6-GES; CD19, CD45RA, CCL22, KLRK1, SOX11, SIGLEC5) correlated with improved outcome after axi-cel (HR: 0.27, 95% CI: 0.16–0.44 for EFS), representing lymphomas with abundant target antigen (CD19) expression, adhesion molecules, and relatively low immune infiltration mostly composed of cytotoxic lymphocytes (T and NK cells) and DCs, and secondly, a 17-gene/transcript signature (17-GES; CD45RO, BCL2, IL-18R1, TNFSF4 [OX40L], KLRB1 [CD161], KIR3DL2, ITGB8, DUSP5, GPC4, PSMB5, RPS6KB1, SERPINA9, NBN,GLUD1, ESR1, ARID1A, and SLC16A1) correlated with disease progression after axi-cel (HR: 6.12, 95% CI: 3.57–10.50 for EFS), consistent with high immune inflammation and escape mechanisms, such as the upregulation of genes involved in repair of damaged DNA or chromatin remodeling, inhibition of apoptosis, and a metabolically restrictive environment. These signatures did not correlate with outcome in the standard-of-care arm of ZUMA-7 (chemotherapy, followed by transplant) or frontline therapy, supporting their predictive rather than prognostic value. The findings were technically reproduced in a subset of ZUMA-7 samples profiled by RNA-seq (axi-cel, n=124; SOC, n=125). The 6-GES was reduced, whereas the 17-GES was elevated at progression post axi-cel, consistent with the notion that these signatures represent features relevant for response and resistance to CAR T-cell therapy.ConclusionOur transcriptomic analysis identified gene expression signatures potentially predictive of outcome with CD19-directed CAR T-cell therapy, and these findings are informative for risk stratification and development of next-generation products.
Efficacy and safety of Prunella vulgaris L. combined with antithyroid drugs for hyperthyroidism: a systematic review and meta-analysis
Authors: Wei Maoying , Zhao Qiyao , Yuan Mingyi , Fan Yuyun , Li Mingdi 
Published: February 2025

ObjectiveTo systematically evaluate the efficacy and safety of Prunella vulgaris L. (PVL) preparations combined with antithyroid drugs (ATDs) for the treatment of hyperthyroidism.MethodsEight Chinese and English databases were searched for randomized controlled trials (RCTs) comparing PVL preparations combined with ATDs and ATDs for hyperthyroidism treatment. The Cochrane risk-of-bias assessment tool was used to evaluate the quality of included studies. Statistical analyses were performed using the Revman 5.3 software. Stata software (version 16.0) was used to detect publication bias. The GRADE system was used to assess the level of evidence.ResultsSeventeen studies were analyzed. The total sample size was 1,366 patients. Meta-analysis revealed that treatment with PVL preparations in combination with ATDs effectively reduced free triiodothyronine [standardized mean difference (SMD) = −0.98, 95%CI (−1.39, −0.57), P &lt; 0.00001], free thyroxine [SMD = −0.82, 95% confidence interval (CI) (−1.16, −0.47), P &lt; 0.00001], thyrotropin receptor antibody [SMD = −1.11, 95%CI (−1.52, −0.71), P &lt; 0.00001], thyroid isthmus thickness [mean difference (MD) = −0.13, 95%CI (−0.15, −0.10), P &lt; 0.00001], width of left thyroid lobe [MD = −0.22, 95%CI (−0.27, −0.17), P &lt; 0.00001], thickness of left thyroid lobe [MD = - 0.22, 95%CI (−0.33, −0.10), P = 0.0003], length of left thyroid lobe [MD = −0.63, 95%CI (−0.79, −0.47), P &lt; 0.00001], width of right thyroid lobe [MD = −0.21, 95%CI (−0.26, −0.16), P &lt; 0.00001], thickness of right thyroid lobe [MD = −0.27, 95%CI (−0.32, −0.22), P &lt; 0.00001], length of right thyroid lobe [MD = −0.45, 95%CI (−0.61, −0.28), P &lt; 0.00001], incidence of adverse events [risk ratio (RR) = 0.34, 95%CI (0.24, 0.50), P &lt; 0.00001], tumor necrosis factor-α [SMD = −2.05, 95%CI (−2.85, −1.25), P &lt; 0.00001], and increasing thyroid-stimulating hormone [SMD = 0.71, 95%CI (0.43, 0.99), P &lt; 0.00001], and interleukin-10 [MD = 1.73, 95%CI (1.35, 2.10), P &lt; 0.00001] better than that of ATDs alone. Combination therapy with PVL preparations was comparable to the efficacy of ATDs alone in improving relapse rates and interleukin-6 and interferon gamma levels.ConclusionTreatment of hyperthyroidism with PVL preparations in combination with ATDs was superior to treatment with ATDs alone in terms of improvements in thyroid function, thyroid antibodies, thyroid gland size, inflammation, and incidence of adverse events. However, owing to the low strength of evidence from the included studies, this conclusion requires further validation in more high-quality RCTs.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42024572591.