bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissemination intelligence information on social media
Authors: Liao Hao , Zhang Jiwei , Zhang Wei , Zhou Mingyang , Wu Xiaoqun , Chao Naipeng 
Published: February 2025

Abstract
Social media platforms, as complex socio-technical ecosystems, have reshaped the way individuals access, share, and interpret information. The interplay of diverse users, rapid content flow, and intricate network structures exemplifies the complexity science domain, where dynamic interactions give rise to emergent patterns of influence and opinion formation. In this perspective, we review three key areas of intelligent information dissemination on social media: (1) rumor and fake news detection, (2) competitive dissemination, and (3) the identification of influential nodes. We highlight recent advancements that leverage complexity-aware models, graph-based algorithms, and deep learning techniques to manage content credibility and guide the spread of verified information. Finally, we propose future research directions that bridge theory and practice, ultimately contributing to a more informed, reliable, and adaptive digital information ecosystem.
Deciphering sperm-carried IGF2 Transcript Variants: Cloning, qPCR Detection, and Variant Analysis
Authors: Cannarella Rossella , Suryavanshi Mangesh , Calogero Aldo E , Desai Nina , Lundy Scott D , Miller Aaron W 
Published: February 2025

Abstract

Background
The insulin-like growth factor 2 (IGF2) gene, a paternally imprinted gene inactive in oocytes, plays a vital role in early embryo development. While five IGF2 variants have been described, the specific variants expressed in human spermatozoa compared to granulosa cells (GCs) remain unclear.

Objective
To characterize the quantity and variants of IGF2 transcripts expressed in human spermatozoa.

Methods
Post-gradient sperm samples were collected from two healthy, fertile men with normal semen parameters, while GCs were isolated following an oocyte retrieval procedure of a woman undergoing in vitro fertilization due to male factor infertility. RNA extraction, cDNA synthesis, PCR amplification, and cloning were performed. PCR products were ligated into PCR4-TOPO vectors and transformed into Escherichia coli DH-10α. A total of 96 positive clones (32 per sample) were characterized via Sanger sequencing to identify variants. qPCR with gene-specific primers analyzed transcript quantities, SNPs, product sizes, and melting temperatures.

Results
Of the 96 true-positive IGF2 cDNA clones, 14 distinct variants were identified, including deletions, insertions, and SNPs, resulting in amino acid sequence changes. Two common variants were present in both sperm and GCs, while two were GC-specific, and the remaining were exclusive to spermatozoa. Some clustered with known NCBI variants, while others formed two novel phylogenetic clusters.

Conclusion
This study expands the repertoire of IGF2 variants and highlights differences between spermatozoa and GC transcripts. It is the first to analyze IGF2 variants in sperm from fertile men, paving the way for future research into their role in embryogenesis.
Glucose-Dependent Insulinotropic Polypeptide in Incretin Physiology: Role in Health and Disease
Authors: Wolfe M Michael , Boylan Michael O , Chin Wiliam W 
Published: February 2025

Abstract
Glucose-dependent insulinotropic polypeptide (GIP) is a 42-amino acid hormone that is synthesized and released from upper intestinal enteroendocrine K-cells in response to the ingestion of glucose or fat. The structure of GIP places it in the secretin/vasoactive intestinal polypeptide family of gastrointestinal regulatory peptides. Although originally named “gastric inhibitory polypeptide” on the basis of its ability to inhibit gastric acid secretion, GIP accounts for 60%-80% of the postprandial insulin response, consistent with the notion that this regulatory peptide constitutes the principal physiological incretin. Under normal conditions, GIP plays a major role in nutrient deposition and storage, both directly through its insulin mimetic properties and indirectly by enhancing insulin release. GIP is overexpressed in obese individuals, which may exacerbate insulin resistance manifested by many patients with type 2 diabetes mellitus. Enhanced postprandial secretion of GIP also initiates a vicious cycle characterized by increased nutrient uptake and storage in adipocytes, leading to insulin resistance and hyperinsulinemia, which then further increases adipocyte nutrient uptake and storage. Despite the deleterious consequences of GIP overexpression, when combined with glucagon-like peptide-1 analogues, GIP agonism has been demonstrated to provide benefit in treating obesity by mechanisms currently not fully elucidated. In contrast, consistent with the etiologic role of GIP overexpression in the pathogenesis of obesity, both genetic abrogation and immunoneutralization of GIP signaling have been shown to reduce the development of obesity in preclinical models. Whether these beneficial effects of GIP antagonism will be extended to humans needs to be determined.
Treatment with Evinacumab Links a New Pathogenic Variant in the LPL Gene to Persistent Chylomicronemia
Authors: Larouche Miriam , Banerjee Poulabi , Brisson Diane , Pordy Robert , Gaudet Daniel 
Published: February 2025

Abstract

Background
Persistent chylomicronemia is caused by lipoprotein lipase deficiency (LPLD) or lack of LPL bioavailability. This disorder is characterized by plasma triglyceride (TG) levels above 10mmol/L, increased acute pancreatitis risk and features of the familial chylomicronemia syndrome (FCS). Evinacumab is an angiopoietin-like protein 3 (ANGPTL3) monoclonal antibody (mab) and its efficacy in decreasing plasma TG levels depends on LPL bioavailability.

Objective
To identify FCS-causing pathogenic variants in patients with persistent chylomicronemia treated with evinacumab.

Methods
A phase II clinical trial was conducted with evinacumab in patients with severe hypertriglyceridemia. Plasma TG values were measured at baseline and every 2 weeks for 24 weeks. Three FCS patients homozygotes for a P234L pathogenic variant in the LPL gene (HoLPL P234L) known to be associated with low post-heparin LPL activity (proven LPLD) participated in the study and were used as tracers. Genotype-specific efficacy of evinacumab to decrease TG levels in other participants was compared to that achieved in HoLPL P234L patients.

Results
After 24 weeks of evinacumab treatment, TG levels decrease &lt;20% in HoLPL P234L patients known to lack LPL. Similarly, a participant homozygote for a E282X variant in the exon 6 of the LPL gene that was suspected to be pathogenic due to its location did not respond to evinacumab (TG decrease &lt;10% and remained &gt; 10 mmol/L).

Conclusion
The efficacy of ANGPTL3 inhibitors in decreasing TG levels is LPL-dependent. Poor response to evinacumab supports the evidence that the E282X variant in the LPL gene is pathogenic and associated with persistent chylomicronemia (FCS phenotype).
Quality of life of patients with alcohol-associated cirrhosis
Authors: Sharma Tarika , Kang Harmeet Kaur , Shalimar  
Published: February 2025

Background: The incidence of alcohol-associated cirrhosis is increasing as a result of the ongoing rise in worldwide alcohol consumption. The current study was undertaken to assess the quality of life (QoL) of individuals with alcohol-associated cirrhosis and explore the association of this QoL measure with various clinicodemographic variables. Methods: A cross-sectional survey was conducted on individuals with alcohol-associated cirrhosis who visited the outpatient department of a tertiary care hospital for treatment from October to December 2023. Patients were screened for alcohol use with the Alcohol Use Disorders Identification Test (AUDIT) and QoL was measured through the Chronic Liver Disease Questionnaire. Clinicodemographic variables were also assessed. Statistics such as frequency, percentage, independent t-test, ANOVA and Bonferroni tests were used for data analysis. Results: Survey results showed that most of the participants (85.6%) had a poor quality of life; the mean score of overall QoL of the study cohort was 3.79±0.95. Analysis at QoL domain level showed that the ‘worry’ domain was the most impacted (mean score 2.88±0.76) followed by ‘Abdominal symptoms’ (mean score 3.32±1.39). QoL scores were found to be statistically significantly associated with AUDIT scores (F=14.328, P<0.001). Post-hoc analysis using the Bonferroni test showed that patients who were alcohol dependent had poorer QoL compared to abstainers or low-risk consumers. Also, patients who were not working because of their disease condition were found to have poor QoL compared to their working counterparts. Conclusions: This study suggests that most patients with alcohol-associated cirrhosis have a poor QoL. Patients who were alcohol dependent had poor QoL scores compared to abstainers or low-risk consumers of alcohol. There is a strong need for motivational enhancement, rehabilitation counselling and alcohol use disorder treatment in liver disease care settings. Healthcare professionals must make every effort to help these patients achieve complete abstinence. Approaches such as telehealth could be used by healthcare professionals for ongoing patient monitoring and motivation to make every effort in helping patients achieve complete abstinence.
Predictive Value of Serum N-Terminal Pro-Brain Natriuretic Peptide, D-Dimer, Albumin Combined with T-Cell Subsets in Detecting Coronary Artery Damage in Children with Kawasaki Disease
Authors: Wang Yanfei , Xing Jing , Gao Hongbo , Zhao Huanhuan , Li Mengjia , Liu Zhenkui 
Published: February 2025

Aims/Background Kawasaki disease (KD) is the common acute, self-limiting vasculitis in children, often affecting coronary arteries, which may lead to coronary artery dilation, stenosis, or in severe cases, myocardial infarction. This study aimed to identify new approaches for reducing or preventing coronary artery lesions (CAL) in KD patients by analyzing specific serological markers across various paediatric groups. Methods Clinical data were collected from 100 children diagnosed with Kawasaki disease (KD) admitted at First Affiliated Hospital of Hebei North University between May 2023 and June 2024. These children were divided into two groups based on coronary artery injury status: Occurrence group (n = 31) and Non-occurrence group (n = 69). Additionally, data from 100 children with acute upper respiratory tract infections (URTI) and 100 healthy children who underwent routine physical examination during the same period (Healthy group) were included for comparison. Serum levels of N-terminal pro-brain natriuretic peptide (NT-proBNP), D-dimer (D-D), albumin (ALB), and T-cell subsets were measured and compared across groups to evaluate their clinical utility in diagnosing coronary artery damage in KD. Results NT-proBNP and D-D levels were highest in KD children with coronary artery injury and lowest in the healthy group, while ALB levels were lowest in KD children with coronary artery injury and highest in the healthy group, with statistically significant differences (p < 0.001). Analysis of T-cell subsets revealed that cluster of differentiation (CD)3+, CD4+, and CD4+/CD8+ levels were highest in the Healthy group, while CD8+ levels were highest in the Occurrence group, with statistically significant differences (p < 0.001). The combined diagnostic model demonstrated an area under the curve (AUC) value of 0.885 (95% CI: 0.829–0.941), showing higher specificity and AUC value compared to each marker individually. Conclusion The combination of serum NT-proBNP, D-D, ALB, and T-cell subsets offers valuable predictive insights for coronary artery damage in KD children and may serve as an auxiliary diagnostic tool.
Combining Circulating Tumour DNA with Clinical Pathological Risk Factors for Developing Peritoneal Metastasis Prediction Model in Patients with Colorectal Cancer
Authors: Han Bohan , Hu Huabin , Zhang Jianwei , Xie Xiaoyu , Deng Yanhong 
Published: February 2025

Aims/Background Peritoneal metastasis in colorectal cancer (CRC) indicates a poor prognosis for patients. Circulating tumour DNA (ctDNA) effectively predicts recurrence and metastasis. Therefore, this study aims to construct a predictive model for peritoneal metastasis by integrating ctDNA with clinicopathological factors in stage I–III CRC patients. Methods We conducted a retrospective analysis of 299 CRC patients who underwent ctDNA detection at The Sixth Affiliated Hospital, Sun Yat-sen University between January 2010 and December 2022. Patients were randomly divided into training (n = 209) and validation (n = 90) sets in a 7:3 ratio using a random number table method. The least absolute shrinkage and selection operator (LASSO) regression model optimized feature selection, and multivariable logistic regression constructed the predictive model. Results Among the study cohort, 59 patients were ctDNA-positive. Postoperative ctDNA positivity was associated with an 8.522-fold increased risk of peritoneal metastasis (p < 0.001, odds ratio (OR) 8.522, 95% confidence interval (CI) 4.371–16.615). The model included preoperative carbohydrate antigen 125 (CA-125), pathological lymph node staging, perineural invasion, and ctDNA levels, achieving an area under the curve (AUC) of 0.808 (95% CI 0.727–0.888) in the training set and 0.784 (95% CI 0.658–0.910) in the validation set. Conclusion This model can accurately identify high-risk patients for peritoneal metastasis in postoperative CRC, facilitating early detection and timely intervention.
Recent climate change strongly impacted the population dynamic of a North American insect pest species
Authors: Boulanger Yan , Desaint Adèle , Martel Véronique , Marchand Maryse , Massoda Tonye Salomon , Saint-Amant Rémi , Régnière Jacques 
Published: February 2025

Climate change is redefining the dynamics of forest ecosystems globally, particularly through its impact on forest pest populations such as the spruce budworm (SBW, Choristoneura fumiferana [Clem.]), a major defoliator in North American boreal forests. This study investigates the shifts in the population dynamics of spruce budworm across its range in response to recent climate change. We used a process-based, temperature-dependent ecophysiological model combined with the ERA5 reanalysis to assess changes in SBW phenology, reproduction rate, winter survival and population growth rates from 1950 to 2022 across North America. Our findings demonstrate a pronounced northward expansion of suitable climate conditions for SBW, accompanied by earlier phenological events and increased reproduction rates in northern regions. Conversely, the southern parts of its range are experiencing increased winter mortality due to warmer temperatures. This study highlights the significant impact of elevated temperatures, particularly during critical developmental windows such as spring and summer, which are pivotal for spruce budworm survival and reproduction. Additionally, our results reveal that the observed shifts in pest dynamics are more strongly driven by climate change than by changes in landscape composition and structure. We estimated that suitable growth rates have shifted northward by over 68 km on average, but this shift reached more than 200 km in the easternmost portions of its range. Climate-induced shift in suitable conditions for SBW underscores the need for adaptive forest management strategies that consider the rapid ecological changes and the potential for increased forest vulnerability due to climatic and biotic stressors. This study provides vital insights that can inform adaptive management ensuring the sustainability of forest ecosystems in the face of ongoing climate change.
Barriers to participation in Iran’s Integrated Geriatric Care Program: A qualitative study
Authors: Nazaripanah Neda Sadat , Nadrian Haidar , Bahrevar Vali , Lotfalinezhad Elham , Hashemiparast Mina 
Published: February 2025

Background
A significant number of older adults still have unfulfilled needs related to healthcare and social services. Policymakers and health administrators can turn this challenge into a golden opportunity through proper planning and policymaking. This study aimed to identify barriers to participation in Iran’s “Integrated Geriatric Care Program (IGCP)”.

Methods
A qualitative study with conventional content analysis approach was conducted in Yazd City, Iran, from January to March 2023. A purposive sampling method with maximum variation was used. Nineteen healthcare providers and community-dwelling older adults participated in the study. Data were collected using semi-structured in-depth interviews until data saturation and were concurrently analyzed.

Results
Nine older persons (3 women and 6 men) with a Mean±SD age of 71.33±5.75 and ten healthcare providers (6 women and 4 men) with a Mean±SD age of 38.2±9.16 participated in the study. Two main categories, with ten sub-categories, emerged from the data analysis. The main categories were “personal challenges of older adults” and “structural defects of Integrated Geriatric Care Program”.

Conclusion
The participation of community-dwelling older adults in the Iranian IGCP is constrained by a combination of personal and structural factors. Our findings seem to be informative for health policymakers and health practitioners who aim to enhance the quality and quantity of healthcare services for older adults in developing communities.
Long-term consumption of hydrogen-rich water provides hepatoprotection by improving mitochondrial biology and quality control in chronically stressed mice
Authors: He Qi , Lan Xiang , Ding Mengyuan , Zhang Na 
Published: February 2025

Background
Chronic stress has emerged as a prevalent facet of contemporary existence, significantly jeopardizing overall bodily health. The liver, a pivotal organ responsible for metabolic equilibrium, is particularly vulnerable to its adverse effects. This study delves into the hepatoprotective properties of extended consumption of HRW in mice subjected to chronic stress.

Methods
Mice subjected to chronic stress via CUMS and HRW administration for seven months underwent liver pathological examination. Key liver function indicators (AST, ALT), oxidative stress markers (SOD, CAT, GSH), and markers related to lipid peroxidation and ferroptosis (MDA, Fe) were measured using standard kits. ELISA determined corticosterone and 4-HNE levels. Immunofluorescence evaluated ROS, Nrf2, and apoptosis in liver tissues. Western blotting analyzed markers for ferroptosis (GPX4, SLC7A11, HO-1, Nrf2), apoptosis (Bax, Bcl-2, Cytc, Caspase-3, Caspase-8), mitochondrial biogenesis (Nrf1, PGC-1α, Tfam), and quality control (Drp1, Fis1, Mfn1, Mfn2, OPA1, PINK1, Parkin, LC3 I/II).

Results
The findings indicate a noteworthy improvement in liver health among mice exposed to HRW, as evidenced by histological analysis. Furthermore, the consumption of HRW exhibited hepatoprotection, as evidenced by the normalization of AST and ALT levels. Mechanistically, our results indicate that HRW elevates the levels of SOD, CAT, and GSH, while effectively clearing ROS within mitochondria. It was observed led to a regulation in the expression of mitochondrial quality control proteins, consequently improving mitochondrial biogenesis (Nrf1, PGC-1α, Tfam), and increasing ATP production. Furthermore, HRW decreased Cytc, Bax, Caspase-3, and Caspase-8 levels, and increasing the expression of Bcl-2. Additionally, HRW reduced MDA and 4-HNE levels, alleviating ferroptosis through the Nrf2/HO-1 pathway, and upregulating the expression of GPX4 and SLC7A11. By mitigating hepatocyte death through the aforementioned mechanisms, HRW fulfills its crucial role in safeguarding liver health.

Conclusions
This study reveals that long-term hydrogen-rich water (HRW) consumption provides significant hepatoprotection in mice under chronic stress. HRW normalizes liver enzyme levels, enhances antioxidant capacity, and reduces lipid peroxidation and ferroptosis. It improves mitochondrial biogenesis, function, and ATP production, and attenuates apoptosis by modulating related proteins. Behavioral tests show HRW alleviates stress-induced anxiety and enhances exploratory behavior. These findings suggest HRW is a promising non-invasive intervention for preventing and treating stress-related liver disorders by targeting oxidative stress and mitochondrial dysfunction.
Combining 3D printing technology with customized metal plates for the treatment of complex acetabular fractures: A retrospective study
Authors: Xu RongDa , Zhang He , Duan SiYu , Liang HaiRui , Sun Ming , Wen Hang , Liu HanFei , Zhou XueTing , Cai ZhenCun 
Published: February 2025

Purposes
The purpose of this study is to evaluate the clinical outcomes of combining 3D printing technology with customized metal plates in the treatment of complex acetabular fractures.

Methods
A retrospective study was conducted on 42 patients with complex acetabular fractures treated at our hospital between September 1, 2020 and May 31, 2022. The patients were divided into two groups: the conventional group and the 3D printing group, with 21 individuals in each group.The conventional group underwent surgery using traditional surgical techniques, with appropriate bending and adjustment of the metal plates during the procedure. In the 3D printing group, preoperative 3D printing technology was utilized to create a physical model of the fracture, and individualized metal plates were customized based on the model after virtual reduction. Comparison was made between the two groups of patients regarding surgical approach, operative time, instrument handling time, intraoperative blood loss, number of fluoroscopy scans, fracture healing time, quality of fracture reduction postoperatively, hip joint function at 12 months postoperatively, and occurrence of complications during the follow-up period.

Results
The 3D printing group showed significantly shorter surgical time (124.76±12.89 minutes vs. 174.05±12.51 minutes), instrument operation time (44.57±5.32 minutes vs. 62.9±7.47 minutes), intraoperative blood loss (337.38±51.95 mL vs. 545.24±74.39 mL), and intraoperative fluoroscopy frequency (8.25±1.18 times vs. 10.52±1.6 times) compared to the conventional group (P<0.001). The postoperative fracture reduction quality in the 3D printing group was good in 95.24% (20/21) of cases, significantly higher than the 61.90% (13/21) in the conventional group (P = 0.02). The excellent and good hip function rate in the 3D printing group was 90.48% (19/21), which was also significantly higher than 57.14% (12/21) in the conventional group (P = 0.01). No significant difference was observed between the two groups in fracture healing time (13.95±1.07 weeks vs. 13.81±1.17 weeks) and complication rate (9.52% vs. 28.57%) (P = 0.14; P = 0.24).

Conclusion
The application of 3D printing technology in conjunction with individualized customization of metal plates for the treatment of complex acetabular fractures can shorten surgical and instrument handling time, reduce intraoperative blood loss, and improve the quality of fracture reduction as well as the recovery of hip joint function.These results provide new insights and technical support for the treatment of complex acetabular fractures.