bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of alteration of caveolin‐1 expression on doxorubicin‐induced apoptosis in H9c2 cardiac cells
Authors: Takaguri Akira , Kamato Maiko , Satoh Yoshiaki , Ohtsuki Kazuaki , Satoh Kumi 
Published: April 2015

AbstractDoxorubicin is an anthracycline antibiotic widely used in cancer treatment. Although its antitumor efficacy appears to be dose dependent, its clinical use is greatly restricted by the development of cardiotoxicity associated with apoptosis. Although caveolin‐1, the major structural protein in caveolae, can positively or negatively regulate apoptosis depending on the stimulus or cell types, the contribution of caveolin‐1 to doxorubicin‐induced apoptosis remains unknown. This study was performed to identify the regulatory role of caveolin‐1 on doxorubicin‐induced apoptosis in H9c2 cardiac cells using a genetic approach. Caveolin‐1 knockdown with a short hairpin (sh) RNA adenovirus, but not overexpression of wild‐type caveolin‐1, resulted in a marked inhibition of doxorubicin‐induced caspase‐3 cleavage. However, caveolin‐1 knockdown tended to protect against doxorubicin‐induced decrease in cell viability, but it did not significantly reverse cell death induced by doxorubicin. Doxorubicin stimulated the phosphorylation of p38 and extracellular signal regulated kinase (ERK). Doxorubicin‐induced caspase‐3 cleavage was inhibited by U0126, a MEK inhibitor or SB203580, a p38 inhibitor. Caveolin‐1 knockdown markedly inhibited doxorubicin‐induced p‐38 phosphorylation but not ERK‐mediated p‐53 phosphorylation in H9c2 cardiac cells. Our results suggest that reduced caveolin‐1 expression plays an anti‐apoptotic role in doxorubicin‐induced apoptosis but that it is insufficient to prevent such an apoptosis in H9c2 cardiac cells.
Untersuchungen zur Steigerung der Trocknungsgeschwindigkeit der Gefriertrocknung
Authors: Pliske Roland , Müller Ulrich , Kohlus Reinhard 
Published: April 2015

AbstractDie Gefriertrocknung bietet gegenüber anderen Trocknungsmethoden den Vorteil einer schonenden Behandlung. Ein Nachteil ist jedoch die längere Trocknungszeit. Abhilfe kann diesbezüglich die dynamische Gefriertrocknung schaffen. Hierbei wird der Wärmeübergang auf das Produkt verbessert und die sich bildende Trockenstoffschicht wird permanent entfernt. Somit wird der Wärme‐ und Stofftransportwiderstand der Trockenschicht reduziert und es wird in der Nähe der Eiskristalle getrocknet. Dies bringt eine Steigerung der Trocknungsgeschwindigkeit mit sich, da im ersten Trocknungsabschnitt getrocknet werden kann.
Prediction with missing data via Bayesian Additive Regression Trees
Authors: Kapelner Adam , Bleich Justin 
Published: April 2015

AbstractWe present a method for incorporating missing data into general prediction problems which use nonparametric statistical learning. We focus on a tree‐based method, Bayesian Additive Regression Trees (BART), enhanced with “Missingness Incorporated in Attributes,” a recently proposed approach for incorporating missingness into decision trees. This procedure extends the native partitioning mechanisms found in tree‐based models and does not require imputation. Simulations on generated models and real data indicate that our procedure offers promise for both selection model and pattern‐mixture frameworks as measured by out‐of‐sample predictive accuracy. We also illustrate BART's abilities to incorporate missingness into uncertainty intervals. Our implementation is readily available in the R package bartMachine. The Canadian Journal of Statistics 43: 224–239; 2015 © 2015 Statistical Society of Canada
Assertive Anger Mediates Effects of Dialectical Behaviour‐informed Skills Training for Borderline Personality Disorder: A Randomized Controlled Trial
Authors: Kramer Ueli , Pascual‐Leone Antonio , Berthoud Laurent , de Roten Yves , Marquet Pierre , Kolly Stéphane , Despland Jean‐Nicolas , Page Dominique 
Published: April 2015

Dialectical behaviour therapy (DBT)‐informed skills training for borderline personality disorder (BPD) aims at the development of specific emotion regulation skills in patients, particularly with regard to the regulation of problematic anger. While the effects of dialectical behaviour skills training have been shown, their processes of change are rarely examined. Neacsiu, Rizvi and Linehan (2010) found that patient's self‐reported use of emotion regulation skills was a mediator of therapeutic change in these treatments; however, they found no effect for problematic anger. From an integrative perspective on anger (Pascual‐Leone & Greenberg, 2007; Pascual‐Leone & Paivio, 2013), there are several forms of anger, varying in their degree of therapeutic productivity. The present add‐on randomized controlled trial included n = 41 patients with BPD (n = 21 DBT‐informed skills training versus n = 20 treatment as usual). The first study examined the outcome of the DBT‐informed skills training encompassing basic components of training in mindfulness, distress tolerance, interpersonal effectiveness and emotion regulation. Results showed that symptom reduction was significantly greater in the DBT‐informed skills training, compared with the treatment as usual. The second study used process assessment, for which all patient completers underwent a 50‐min‐long psychological interview both early and late in treatment, which was rated using the Classification of Affective Meaning States. DBT‐informed skills training produced increased levels of primary ‘assertive’ anger, as compared with the treatment as usual, whereas no effect was found for ‘rejecting’ secondary anger. Most importantly, we showed that changes in assertive anger mediated the reported symptom reduction, in particular in patient's social roles. We discuss these results in the context of underlying mechanisms of change in DBT skills group treatments, in particular towards developing more productive forms of anger in this patient population. Copyright © 2015 John Wiley & Sons, Ltd.Key Practitioner Message

A 20‐session dialectical behaviour therapy (DBT)‐informed skills training is a promising adjunct intervention for patients with borderline personality disorder, in particular for reducing problems related to social role.
Increases in assertive anger mediate the effects of DBT‐informed skills training, whereas rejecting anger remains unchanged over the course of treatment.
Short‐term objectives for intervention might involve the specific increase of assertive anger in BPD, by using DBT‐informed skills training; long‐term objectives for intervention might involve a specific decrease of rejecting anger in BPD.
Motor neuron‐expressed microRNAs 218 and their enhancers are nested within introns of Slit2/3 genes
Authors: Punnamoottil Beena , Rinkwitz Silke , Giacomotto Jean , Svahn Adam J. , Becker Thomas S. 
Published: April 2015

SummarymiR218‐1 and miR218‐2 are embedded in introns of SLIT2 and SLIT3, respectively, an arrangement conserved throughout vertebrate genomes. Both miR218 genes are predicted to be transcribed in the same orientation as their host genes and were assumed to be spliced from Slit2/3 primary transcripts. In zebrafish miR218 is active in cranial nerve motor nuclei and spinal cord motor neurons, while slit2 and slit3 are expressed predominantly in the midline. This differential expression pattern suggested independent regulation of miR218 genes by distinct enhancers. We tested conserved noncoding elements for regulatory activity by reporter gene transgenesis in zebrafish. Two human enhancers, 76 kb and 130 kb distant from miR218‐2, were identified that drove GFP expression in zebrafish in an almost complete miR218 expression pattern. In the zebrafish slit3 locus, two enhancers with identical activity were discovered. In human SLIT2 one enhancer 52 kb upstream of miR218‐1 drove an expression pattern very similar to the enhancers of miR218‐2. This establishes that miR218‐1/‐2 regulatory units are nested within SLIT2/3 and that they are duplicates of an ancestral single locus. Due to the strong activity of the enhancers, unique transgenic lines were created that facilitate morphological and gene functional genetic experiments in motor neurons. genesis 53:321–328, 2015. © 2015 Wiley Periodicals, Inc.
Ecological gradients driving the distribution of four Ericaceae in boreal Quebec, Canada
Authors: Thiffault Nelson , Grondin Pierre , Noël Jean , Poirier Véronique 
Published: April 2015

AbstractUnderstory species play a significant role in forest ecosystem dynamics. As such, species of the Ericaceae family have a major effect on the regeneration of tree species in boreal ecosystems. It is thus imperative to understand the ecological gradients controlling their distribution and abundance, so that their impacts can be taken into account in sustainable forest management. Using innovative analytical techniques from landscape ecology, we aimed to position, along ecological gradients, four Ericaceae found in the boreal forest of Quebec (Canada) (Rhododendron groenlandicum, Kalmia angustifolia, Chamaedaphne calyculata, and Vaccinium spp), to regionalize these species into landscape units relevant to forest management, and to estimate the relative importance of several ecological drivers (climate, disturbances, stand attributes, and physical environment) that control the species distribution and abundance. We conducted our study in boreal Quebec, over a study area covering 535,355 km2. We used data from 15,339 ecological survey plots and forest maps to characterize 1422 ecological districts covering the study region. We evaluated the relative proportion of each ericaceous species and explanatory variables at the district level. Vegetation and explanatory variables matrices were used to conduct redundancy, cluster, and variation partitioning analyses. We observed that ericaceous species are mainly distributed in the western part of the study area and each species has a distinct latitudinal and longitudinal gradient distribution. On the basis of these gradients, we delimited 10 homogeneous landscape units distinct in terms of ericaceous species abundance and environmental drivers. The distribution of the ericaceous species along ecological gradients is closely related to the overlaps between the four sets of explanatory variables considered. We conclude that the studied Ericaceae occupy specific positions along ecological gradients and possess a specific abundance and distribution controlled by the integration of multiple explanatory variables.
Cross‐regulation between cytokine and microRNA pathways in T cells
Authors: Amado Tiago , Schmolka Nina , Metwally Hozaifa , Silva‐Santos Bruno , Gomes Anita Q. 
Published: April 2015

microRNA (miRNA) mediated regulation of protein expression has emerged as an important mechanism in T‐cell physiology, from development and survival to activation, proliferation, and differentiation. One of the major classes of proteins involved in these processes are cytokines, which are both key input signals and major products of T‐cell function. Here, we summarize the current data on the molecular cross‐talk between cytokines and miRNAs: how cytokines regulate miRNA expression, and how specific miRNAs control cytokine production in T cells. We also describe the inflammatory consequences of deregulating the miRNA/cytokine axis in mice and humans. We believe this topical area will have key implications for immune modulation and treatment of autoimmune pathology.
Out‐of‐plane strength reduction of unreinforced masonry walls because of in‐plane damages
Authors: Dolatshahi Kiarash M. , Yekrangnia Mohammad 
Published: April 2015

SummaryThere are numerous studies on the behavior of Unreinforced Masonry (URM) walls in both in‐plane (IP) and out‐of‐plane (OP) directions; however, few aimed at understanding the simultaneous contribution of these intrinsic responses during earthquakes. Undoubtedly, even a strong URM wall shows weakened capacity in the OP direction because of minor cracks and other damages in the IP direction, and this capacity reduction has not yet been accounted for in seismic codes. In this study, performance of three URM walls is evaluated by several numerical analyses in terms of the OP capacity reduction because of IP displacements and failure modes. Several parameters influencing the OP capacity have been studied including aspect ratio, roof boundary condition, IP displacement and IP loading patterns. The results indicate that reduction in the OP capacity of URM walls varies from negligible to very high depending on boundary conditions, IP failure mode and IP damage severity. Moreover, IP loading pattern is more important in walls with higher aspect ratios because of their IP failure modes. Copyright © 2015 John Wiley & Sons, Ltd.
Habits of Practice
Authors: Wickens Corrine M. , Manderino Michael , Parker Jenny , Jung Jinhong 
Published: April 2015

AbstractIn recent years, disciplinary literacy has been at the forefront of adolescent literacy research and practice but has largely focused on the four core content areas: English language arts, social studies, science, and mathematics. Drawing on a physical education lens, this article is a call to expand the definitions, approaches, and framework of disciplinary literacy by examining three critical issues for disciplinary literacy: focus on the disciplinary expert (i.e., thinking like a historian, a mathematician, a scientist, etc.), emphasis on cognition over other forms of engagement and disciplinary practice, and notions of student participation and activity.
Transgenic and cloned animals in the food chain – are we prepared to tackle it?
Authors: Jagadeesan Premanandh , Salem Samara Bin 
Published: April 2015

AbstractTransgenic and cloned animal production for various purposes has been increasing rapidly in recent times. While the actual impact of these animals in the food chain is unknown, the significance of tracking and monitoring measures to curb accidental and or deliberate release has been discussed. Religious perspectives from different faiths and traditions have been presented. Although the concept of substantial equivalence satisfies the technical and nutritional requirements of these products when assessed against comparators, public opinion and religious concerns should also be considered by the regulators while developing policy regulations. In conclusion, measures to prevent real or perceived risks of transgenic and cloned animals in food production require global coordinated action. It is worthwhile to consider establishing effective tracking systems and analytical procedures as this will be a valuable tool if a global consensus is not reached on policy regulation. © 2015 Society of Chemical Industry
Contribution of portal‐drained viscera to heat production in Iberian gilts fed a low‐protein diet: comparison to Landrace
Authors: González‐Valero Lucrecia , Rodríguez‐López José M , Lachica Manuel , Fernández‐Fígares Ignacio 
Published: April 2015

AbstractBACKGROUNDIn terms of whole‐body metabolism, visceral tissues have a disproportionate influence with respect to their masses, and under certain circumstances their high metabolic rate may compromise nutrient availability to the tissues of productive/economic interest. The low energy efficiency of Iberian compared to modern breeds could be partly explained by the contribution of portal‐drained viscera to heat production. The objective of this study was to determine the relative contribution of portal‐drained viscera heat production (PDVHP) to total heat production (THP) in Iberian and Landrace gilts fed a diet with low protein content.RESULTSCompared to Landrace, Iberian gilts (29 ± 0.9 kg body weight) had lower pre‐ and postprandial portal vein blood flow (654 vs. 965, and 746 vs. 1133 mL min−1, respectively; P < 0.01), postprandial PDVHP (3.09 vs. 3.88 kJ h−1 kg−0.75; P < 0.01) and PDVHP/THP ratio (0.08 vs. 0.13; P < 0.05), and greater THP (38.5 vs. 31.8 kJ h−1 kg−0.75; P < 0.05), when offered 0.25 of their daily ration in a 6 h sampling period.CONCLUSIONIn spite of the low energetic efficiency and growth rate of Iberian compared to modern breeds, the contribution of PDV energy expenditure relative to THP was lower in Iberian compared to Landrace gilts in the present study. © 2015 Society of Chemical Industry
DNA damage protection against free radicals of two antioxidant neolignan glucosides from sugarcane molasses
Authors: Asikin Yonathan , Takahashi Makoto , Mizu Masami , Takara Kensaku , Oku Hirosuke , Wada Koji 
Published: April 2015

AbstractBACKGROUNDSugarcane molasses is a potential by‐product of the sugarcane manufacturing industry that is rich in antioxidant materials. The present study aimed to obtain antioxidative compounds from sugarcane molasses and to evaluate their ability to protect DNA from oxidative damage.RESULTSTwo neolignan glucosides were isolated from sugarcane molasses using bioassay and UV spectra monitoring‐guided fractionation. The compounds were elucidated as (7R,8S)‐dehydrodiconiferyl alcohol‐4‐O‐β‐d‐glucoside (1) and (7S,8R)‐simulanol‐9′‐O‐β‐d‐glucoside (2). Neolignan glucoside 2 protected against DNA damage caused by free radicals more effectively than did neolignan glucoside 1 (13.62 and 9.08 µmol L−1 for peroxyl and hydroxyl radicals, respectively, compared to 48.07 and 14.42 µmol L−1). Additionally, neolignan glucoside 2 exhibited superior DNA protection against free radicals compared with various known antioxidative compounds, including p‐coumaric acid, ferulic acid, vanillic acid and epigallocatechin gallate.CONCLUSIONThe isolated neolignan glucosides from sugarcane molasses are able to protect DNA from oxidative damage caused by free radicals. This is the first identification of these two compounds in sugarcane molasses. The sugarcane molasses can therefore be used as potential nutraceutical preventative agents, and the findings may foster the utilization of this by‐product as a bioresource‐based product. © 2015 Society of Chemical Industry
Fowler's syndrome of urinary retention: A retrospective study of co‐morbidity
Authors: Hoeritzauer Ingrid , Stone Jon , Fowler Clare , Elneil‐Coker Suzy , Carson Alan , Panicker Jalesh 
Published: April 2015

AimsTo study the frequency of pain, psychological, or functional disorders in patients with Fowler's syndrome.MethodsWe carried out a retrospective chart review of patients with a diagnosis of Fowler's syndrome attending the Uro‐Neurology centre at the National Hospital for Neurology and Neurosurgery between 2009 and 2013 looking at triggering events, physical and psychological comorbidities.ResultsOf 62 patients with clinical and electromyographic diagnosis of Fowler's syndrome, 31 (50%) had unexplained chronic pain syndromes, 12 (19%) of these were taking opiates. Fifteen (24%) had “functional” neurological symptoms. Abdominopelvic surgery with general anesthesia was the leading trigger (n = 21, 35%).ConclusionsWe found high levels of co‐morbidity with patients having some form of pain (50%), a probable functional disorder (24%), or psychological symptoms (31%). There are several potential explanations for this association including the effect of developing an apparently unexplained distressing condition, confounding effect of opiate use or referral bias. The findings suggest a need for prospective systematic study of comorbidity for this disabling condition. Neurourol. Urodynam. 35:601–603, 2016. © 2015 Wiley Periodicals, Inc.
Survival of a child with spinal muscular atrophy and acute respiratory distress syndrome
Authors: Wampole Anthony , Schroth Mary , Boriosi Juan 
Published: April 2015

SummarySpinal muscular atrophy (SMA), a lower motor neuron anterior horn cell disease, causes significant respiratory morbidity and mortality in children. Acute respiratory distress syndrome (ARDS) accounts for 1–4% of all Pediatric Intensive Care Unit (PICU) admissions. Management outcomes for ARDS in patients with SMA have not been described. We present the case of a 5‐year‐old boy with Type II SMA and ARDS requiring invasive mechanical ventilation. He improved with meticulous management of mechanical ventilation, airway clearance, fluid/nutrition, and sedation/analgesia. He was successfully extubated after 14 days of invasive mechanical ventilation and discharged home after a 20 day hospitalization. Pediatr Pulmonol. 2015; 50:E29–E31. © 2015 Wiley Periodicals, Inc.
Antidepressive‐like effect of a Kampo (traditional Japanese) medicine, kososan (Xiang Su San) in a stress‐induced depression‐like mouse model: Proteomic analysis of hypothalamus
Authors: Nagai Takayuki , Hashimoto Ryoko , Okuda Sari M. , Kodera Yoshio , Oh‐Ishi Masamichi , Maeda Tadakazu , Ito Naoki , Hanawa Toshihiko , Kiyohara Hiroaki , Yamada Haruki 
Published: April 2015

ABSTRACTAimA Kampo medicine, kososan (KS), has been used clinically for the treatment of certain depression‐like symptoms. Our previous studies using the stress‐induced depression‐like model mice, showed that oral KS treatment leads to an antidepressive‐like effect via the normalization of dysfunction of hypothalamic‐pituitary‐adrenal axis. In the present study, proteomic analysis was used to identify brain hypothalamus proteins that are affected by KS treatment.Methods
KS was orally administered to stress‐induced depression‐like model mice at 1.0 g/kg/day for 9 days, and the hypothalamus was then analyzed using agarose 2‐D gel electrophoresis, followed by mass spectrometry‐based protein identification, and then western blot analysis and immunohistochemical analysis.ResultsThe expression of eight proteins was increased or decreased in the hypothalamus of the model mice, but recovered on oral KS treatment. Among them, metabotropic glutamate receptor 2 (mGluR2) and 2′,3′‐cyclic nucleotide 3′‐phosphodiesterase 1 (CNPase1) were identified as depression‐related proteins. Western blot of the hypothalamus confirmed these results and showed that the expression of CNPase2 was opposite to that of CNPase1. On immunohistochemistry, mGluR2 expression in the hypothalamus paraventricular nucleus was downregulated and CNPase2 expression was upregulated in the cerebral fornix in the model mouse, but KS returned them to normal.Conclusion
mGluR2 and CNPase in the hypothalamus are associated with the antidepressive‐like activity of KS.
Preparing English Learners for Effective Peer Review in the Writers' Workshop
Authors: Kim Soo Hyon 
Published: April 2015

AbstractEnglish Language Learners (ELLs) often face challenges when participating in peer review activities in writers' workshops. This article identifies some of the potential difficulties that ELL writers may experience, and provides teachers with strategies to address these problems. The author describes a simple three‐step peer review training model that she has successfully used with her students during writers' workshops. The training model involves establishing a shared set of evaluation criteria and expectations before peer review, supporting ELLs in their formulation and articulation of feedback during peer review, and helping ELLs reflect on the peer feedback they received after peer review. By implementing this three‐step peer review training model, teachers can strengthen the positive effects of using peer review during the writers' workshop, while supporting ELL writers who are still developing their proficiency in English.