bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimizing fucosylation in insect cell‐derived glycoproteins reduces binding to IgE antibodies from the sera of patients with allergy
Authors: Palmberger Dieter , Ashjaei Kazem , Strell Stephanie , Hoffmann‐Sommergruber Karin , Grabherr Reingard 
Published: April 2014

AbstractThe baculovirus/insect cell system has proven to be a very powerful tool for the expression of several therapeutics. Nevertheless, these products sometimes suffer from reduced biological activity and unwanted side effects. Several studies have demonstrated that glycosylation can greatly influence the structure, function, half‐life, antigenicity and immunogenicity of various glycoproteins. Yet, the glycosylation pattern of insect cell‐derived products is not favorable for many applications. Especially, the presence of core α1,3‐linked fucose bears the risk of causing immediate hypersensitivity reactions in patients with allergy. In this study, we evaluated the impact of fucose residues on the allergenic potential of an insect cell‐expressed vaccine candidate. In order to block the GDP‐L‐fucose de novo synthesis pathway, we integrated the Pseudomonas aeruginosa GDP‐6‐deoxy‐D‐lyxo‐4‐hexulose reductase (RMD) gene into a baculovirus backbone. This virus was then used for the expression of soluble influenza A virus hemagglutinin (HA). Expression studies showed that the co‐expression of RMD did not influence the overall level of recombinant protein secretion. We confirmed the result of our strategy by analyzing PNGase A‐released N‐glycans using MALDI‐TOF‐MS. In order to evaluate the biological impact of defucosylation of influenza HA we tested the binding activity of IgE derived from the sera of patients with allergy to the purified antigen. The non‐fucosylated HA showed a 10‐fold decrease in IgE binding levels as compared to wildtype variants.same article under 10.1002/biot.201300061.
Early sac shrinkage predicts a low risk of late complications after endovascular aortic aneurysm repair
Authors: Bastos Gonçalves F , Baderkhan H , Verhagen H J M , Wanhainen A , Björck M , Stolker R J , Hoeks S E , Mani K 
Published: April 2014

Abstract

Background
Aneurysm shrinkage has been proposed as a marker of successful endovascular aneurysm repair (EVAR). Patients with early postoperative shrinkage may experience fewer subsequent complications, and consequently require less intensive surveillance.

Methods
Patients undergoing EVAR from 2000 to 2011 at three vascular centres (in 2 countries), who had two imaging examinations (postoperative and after 6–18 months), were included. Maximum diameter, complications and secondary interventions during follow-up were registered. Patients were categorized according to early sac dynamics. The primary endpoint was freedom from late complications. Secondary endpoints were freedom from secondary intervention, postimplant rupture and direct (type I/III) endoleaks.

Results
Some 597 EVARs (71·1 per cent of all EVARs) were included. No shrinkage was observed in 284 patients (47·6 per cent), moderate shrinkage (5–9 mm) in 142 (23·8 per cent) and major shrinkage (at least 10 mm) in 171 patients (28·6 per cent). Four years after the index imaging, the rate of freedom from complications was 84·3 (95 per cent confidence interval 78·7 to 89·8), 88·1 (80·6 to 95·5) and 94·4 (90·1 to 98·7) per cent respectively. No shrinkage was an independent risk factor for late complications compared with major shrinkage (hazard ratio (HR) 3·11; P &lt; 0·001). Moderate compared with major shrinkage (HR 2·10; P = 0·022), early postoperative complications (HR 3·34; P &lt; 0·001) and increasing abdominal aortic aneurysm baseline diameter (HR 1·02; P = 0·001) were also risk factors for late complications. Freedom from secondary interventions and direct endoleaks was greater for patients with major sac shrinkage.

Conclusion
Early change in aneurysm sac diameter is a strong predictor of late complications after EVAR. Patients with major sac shrinkage have a very low risk of complications for up to 5 years. This parameter may be used to tailor postoperative surveillance.
The influence of COMT Val158Met genotype on the character dimension cooperativeness in healthy females
Authors: Baeken Chris , Claes Stephan , De Raedt Rudi 
Published: April 2014

AbstractObjectivesAlthough the Val158Met catechol‐O‐methyltransferase (COMT) gene has been linked with the temperament dimension Novelty Seeking (NS), new insights in this polymorphism might point to a major role for character features as well. Given that individual life experiences may influence Val158 and Met158 allele carriers differently it has been suggested that the character trait cooperativeness could be implicated.Case reportA homogeneous group of eighty right‐handed Caucasian healthy female university students were assessed with the TCI and genotyped for the COMT Val158Met polymorphism (rs4680). Gene determination showed that eighteen were Val158 homozygotes, forty‐four Val/Met158 heterozygotes, and eighteen were Met158 homozygotes. All were within the same age range and never documented to have suffered from any neuropsychiatric illness. Bonferroni corrected non‐parametric analyses showed that only for the character scale cooperativeness Val158 homozygotes displayed significant higher scores when compared to Met158 homozygotes. No significant differences on cooperativeness scores were found between Val158 and Val/Met158 carriers or between Met158 and Val/Met158 carriers. No differences were observed for the COMT Val158Met polymorphism and the other temperament and character scales.ConclusionsOur findings support the assumption that the Val158Met single nucleotide polymorphism (SNP) influences character traits and not only temperament. Our results add to the notion that Val158 homozygotes are considered to be helpful and empathic and it suggest that these cooperativeness character traits are related to the dopaminergic system.
Perfluorobutyric Acid and Its Monohydrate: A Chirped Pulse and Cavity Based Fourier Transform Microwave Spectroscopic Study
Authors: Thomas Javix , Serrato Agapito , Lin Wei , Jäger Wolfgang , Xu Yunjie 
Published: April 2014

AbstractRotational spectra of perfluorobutyric acid (PFBA) and its monohydrate were studied with a broadband chirped pulse and a narrow‐band cavity based Fourier transform microwave spectrometer, and high‐level ab initio calculations. Extensive conformational searches were performed for both the acid and its monohydrate at the MP2/6‐311++G(2d,p) level of theory. Two and three conformers were predicted to exist for PFBA and its monohydrate, respectively. One set of rotational transitions was observed and assigned for each, PFBA and its monohydrate. Based on the measured broadband spectra, we confidently conclude that only one dominant conformer exists in each case. The orientation of the hydroxyl group in PFBA was determined by using isotopic analysis. Comparison of the observed transition intensities and the calculated electric dipole moment components allowed us to identify the most stable monohydrate conformation, which takes on an insertion hydrogen‐bonding topology. Comparisons to the shorter chain analogues, that is, trifluoroacetic acid, perfluoropropionic acid, and their monohydrates, are made to elucidate the general trend in their conformational preference and binding topologies.
Combinatorial Approach to Chiral Tris‐ligated Carbophilic Platinum Complexes: Application to Asymmetric Catalysis
Authors: Pradal Alexandre , Gladiali Serafino , Michelet Veronique , Toullec Patrick Y. 
Published: April 2014

AbstractA straightforward methodology for the synthesis of libraries of chiral tris‐ligated cationic platinum complexes and their in situ evaluation as asymmetric carbophilic catalysts in a model domino hydroarylation/cyclization reaction of a 1,6‐enyne was developed. A catalyst‐generation process based on a combination of a monodentate and a bidentate phosphorus ligand allowed the formation of 108 chiral complexes. One‐pot screening of the stereoinduction obtained with this library in a test domino addition/cyclization reaction validated this approach and stressed the key role played by the monodentate ligand partner in obtaining high enantioselectivities. In the case of two challenging substrate/nucleophile combinations, the combinatorial approach resulted in a significant gain in enantioselectivity.
Copper‐Catalyzed Dehydrogenative Cross‐Coupling Reaction between Allylic CH Bonds and α‐CH Bonds of Ketones or Aldehydes
Authors: Huang Xing‐Fen , Salman Muhammad , Huang Zhi‐Zhen 
Published: April 2014

AbstractA dehydrogenative cross‐coupling reaction between allylic CH bonds and the α‐CH bond of ketones or aldehydes was developed using Cu(OTf)2 as a catalyst and DDQ as an oxidant. This synthetic approach to γ,δ‐unsaturated ketones and aldehydes has the advantages of broad scope for both ketones and aldehydes as reactants, mild reaction conditions, good yields and atom economy. A plausible mechanism using Cu(OTf)2 as a Lewis acid catalyst was also proposed (DDQ=2,3‐dichloro‐5,6‐dicyano‐1,4‐benzoquinone; Tf=trifluoromethanesulfonate).
Multivalent Recognition of Concanavalin A by {Mo132} Glyconanocapsules—Toward Biomimetic Hybrid Multilayers
Authors: Barboiu Mihail , Mouline Zineb , Silion Mihaela , Licsandru Erol , Simionescu Bogdan C. , Mahon Eugene , Pinteala Mariana 
Published: April 2014

AbstractHerein, we consider Müller’s spherical, porous, anionic, molybdenum oxide based capsule, (NH4)42‐ [{(MoVI)MoVI5O21(H2O)6}12{MoV2O4(CH3COO)}30]⋅10 CH3COONH4⋅ 300 H2O≡(NH4)42⋅1 a⋅crystal ingredients≡1, {Mo132}, as an effective sugar‐decorated nanoplatform for multivalent lectin recognition. The ion‐exchange of NH4+ ions of 1 with cationic‐sugars, D‐mannose‐ammonium chloride (2) or D‐glucose‐ammonium chloride (3) results in the formation of glyconanocapsules (NH4)42−n2n⋅1 a and (NH4)42−m3m⋅1 a. The Mannose (NH4)42−n2n⋅1 a capsules bind selectively Concanavalin A (Con A) in aqueous solution, giving an association avidity constant of ${K{{{\rm multi}\hfill \atop {\rm a}\hfill}}}$=4.6×104 M−1 and an enhancement factor of β=K${{{{\rm multi}\hfill \atop {\rm a}\hfill}}}$/K${{{{\rm mono}\hfill \atop {\rm ass}\hfill}}}$=21.9, reminiscent of the formation of “glycoside clusters” on the external surface of glyconanocapsule. The glyconanocapsules (NH4)42−n2n⋅1 a and (NH4)42−m3m⋅1 a self‐assemble in “hybrid multilayers” by successive layer‐by‐layer deposition of (NH4)42−n2n⋅1 a or (NH4)42−m3m⋅1 a and Con A. These architectures, reminiscent of versatile mimics of artificial tissues, can be easily prepared and quantified by using quartz crystal microgravimetry (QCM). The “biomimetic hybrid multilayers” described here are stable under a continual water flow and they may serve as artificial networks for a greater depth of understanding of various biological mechanisms, which can directly benefit the fields of chemical separations, sensors or storage‐delivery devices.
Activation of Elemental Sulfur by Aluminum Dihydride: Isolation of Mono‐ and Bis(hydrogensulfide) Complexes of Aluminum
Authors: Franz Daniel , Inoue Shigeyoshi 
Published: April 2014

AbstractThe conversions of the dimeric imidazolin‐2‐iminato aluminum dihydride {μ‐LDippAlH2}2 (1) with 1/4 or 1/2 equivalents of S8 gave the elusive hydrogensulfide {μ‐LDippAl(H)SH}2 (2) and the bisthiol {μ‐LDippAl(SH)2}2 (3), respectively (LDipp=NC(N(Dipp)CH)2, Dipp=2,6‐diisopropylphenyl). Notably, these transformations proceeded in the absence of a promoter. The reaction of {μ‐LMesAlH2}2 (4), a less bulky congener of 1, with phenyl disulfide produces the phenyl sulfide {μ‐LMesAl(H)SPh}2 (5; LMes=NC(N(Mes)CH)2, Mes=2,4,6‐trimethylphenyl). The hitherto unknown compounds 2–5 were characterized by using spectroscopic methods and single‐crystal X‐ray diffraction analysis.
Hyperbranched Supramolecular Polymer of Tris(permethyl‐β‐cyclodextrin)s with Porphyrins: Characterization and Magnetic Resonance Imaging
Authors: Sun Mo , Zhang Hengyi , Hu Xinyue , Liu Bowen , Liu Yu 
Published: April 2014

AbstractA hyperbranched supramolecular polymer (HSP) is constructed based on the intermolecular inclusion complexation of bridged tris(permethyl‐β‐cyclodextrin) with MnIII‐porphyrin bearing poly(ethylene glycol) (PEG) side chains (MnIII‐TPP), and characterized by UV/vis absorption spectroscopy, isothermal titration calorimetry (ITC), NMR, dynamic light scattering (DLS), atomic force microscopy (AFM) and transmission electron microscopy (TEM), respectively. The presence of permethyl‐β‐cyclodextrin can stabilize the low‐valent MnII‐TPP. In vitro cell experiment indicates that this HSP has no remarkable cellular toxicity against NIH 3T3 cells. In vitro MR imaging experiment indicates a slightly increased T1 relaxivity for this HSP compared to that for the linear supramolecular polymer.
Microporous Carbon and Mesoporous Silica by Use of Twin Polymerization: An Integrated Experimental and Theoretical Approach to Precursor Reactivity
Authors: Kitschke Philipp , Auer Alexander A. , Löschner Tina , Seifert Andreas , Spange Stefan , Rüffer Tobias , Lang Heinrich , Mehring Michael 
Published: April 2014

AbstractSpirocyclic silicon alkoxides were synthesized by reaction of Si(OMe)4 with derivatives of salicylic alcohol and studied by in situ differential scanning calorimetry with regard to twin polymerization (TP). Both, thermally induced and proton‐assisted TP gave nanostructured hybrid materials composed of a phenolic resin and silica. Carbonization and subsequent treatment with HF(aq) resulted in microporous carbon, whereas oxidation in air provided mesoporous silica. DFT calculations were performed to obtain a more detailed insight into the first reaction steps of proton‐assisted TP and to support the hypothesis of a reactivity scale based on steric and electronic features of the silicon‐containing precursors (twin monomers). The calculated reaction barriers for the initial reaction steps of proton‐assisted TP are qualitatively in accordance with the Hammett constants of the substituents at the salicylate moiety. This result offers a simple method to predict the reactivity for twin monomers.
Combination of Carbon Nitride and Carbon Nanotubes: Synergistic Catalysts for Energy Conversion
Authors: Gong Yutong , Wang Jing , Wei Zhongzhe , Zhang Pengfei , Li Haoran , Wang Yong 
Published: April 2014

AbstractDue to their versatile features and environmental friendliness, functionalized carbon materials show great potential in practical applications, especially in energy conversion. Developing carbon composites with properties that can be modulated by simply changing the ratio of the original materials is an intriguing synthetic strategy. Here, we took cyanamide and multiwalled carbon nanotubes as precursors and introduced a facile method to fabricate a series of graphitic carbon nitride/carbon nanotubes (g‐C3N4/CNTs) composites. These composites demonstrated different practical applications with different weight ratios of the components, that is, they showed synergistic effects in optoelectronic conversion when g‐C3N4 was the main ingredient and in oxygen reduction reaction (ORR) when CNTs dominated the composites. Our experiments indicated that the high electrical conductivity of carbon nanotubes promoted the transmission of the charges in both cases.
Neurotransmitter map of the asymmetric dorsal habenular nuclei of zebrafish
Authors: deCarvalho Tagide N. , Subedi Abhignya , Rock Jason , Harfe Brian D. , Thisse Christine , Thisse Bernard , Halpern Marnie E. , Hong Elim 
Published: April 2014

SummaryThe role of the habenular nuclei in modulating fear and reward pathways has sparked a renewed interest in this conserved forebrain region. The bilaterally paired habenular nuclei, each consisting of a medial/dorsal and lateral/ventral nucleus, can be further divided into discrete subdomains whose neuronal populations, precise connectivity, and specific functions are not well understood. An added complexity is that the left and right habenulae show pronounced morphological differences in many non‐mammalian species. Notably, the dorsal habenulae of larval zebrafish provide a vertebrate genetic model to probe the development and functional significance of brain asymmetry. Previous reports have described a number of genes that are expressed in the zebrafish habenulae, either in bilaterally symmetric patterns or more extensively on one side of the brain than the other. The goal of our study was to generate a comprehensive map of the zebrafish dorsal habenular nuclei, by delineating the relationship between gene expression domains, comparing the extent of left–right asymmetry at larval and adult stages, and identifying potentially functional subnuclear regions as defined by neurotransmitter phenotype. Although many aspects of habenular organization appear conserved with rodents, the zebrafish habenulae also possess unique properties that may underlie lateralization of their functions. genesis 52:636–655, 2014. © 2014 Wiley Periodicals, Inc.
Left–right asymmetry of the gnathostome skull: Its evolutionary, developmental, and functional aspects
Authors: Compagnucci Claudia , Fish Jennifer , Depew Michael J. 
Published: April 2014

SummaryMuch of the gnathostome (jawed vertebrate) evolutionary radiation was dependent on the ability to sense and interpret the environment and subsequently act upon this information through utilization of a specialized mode of feeding involving the jaws. While the gnathostome skull, reflective of the vertebrate baüplan, typically is bilaterally symmetric with right (dextral) and left (sinistral) halves essentially representing mirror images along the midline, both adaptive and abnormal asymmetries have appeared. Herein we provide a basic primer on studies of the asymmetric development of the gnathostome skull, touching briefly on asymmetry as a field of study, then describing the nature of cranial development and finally underscoring evolutionary and functional aspects of left–right asymmetric cephalic development. genesis 52:515–527, 2014. © 2014 Wiley Periodicals, Inc.
Influence of whitebark pine decline on fall habitat use and movements of grizzly bears in the Greater Yellowstone Ecosystem
Authors: Costello Cecily M. , van Manen Frank T. , Haroldson Mark A. , Ebinger Michael R. , Cain Steven L. , Gunther Kerry A. , Bjornlie Daniel D. 
Published: April 2014

AbstractWhen abundant, seeds of the high‐elevation whitebark pine (WBP;Pinus albicaulis) are an important fall food for grizzly bears (Ursus arctos) in the Greater Yellowstone Ecosystem. Rates of bear mortality and bear/human conflicts have been inversely associated withWBPproductivity. Recently, mountain pine beetles (Dendroctonus ponderosae) have killed many cone‐producingWBPtrees. We used fall (15 August–30 September) Global Positioning System locations from 89 bear years to investigate temporal changes in habitat use and movements during 2000–2011. We calculated Manly–Chesson (MC) indices for selectivity ofWBPhabitat and secure habitat (≥500 m from roads and human developments), determined dates ofWBPuse, and documented net daily movement distances and activity radii. To evaluate temporal trends, we used regression, model selection, and candidate model sets consisting of annualWBPproduction, sex, and year. One‐third of sampled grizzly bears had fall ranges with little or no mappedWBPhabitat. Most other bears (72%) had aMCindex above 0.5, indicating selection forWBPhabitats. From 2000 to 2011, meanMCindex decreased and median date ofWBPuse shifted about 1 week later. We detected no trends in movement indices over time. Outside of national parks, there was no correlation between theMCindices forWBPhabitat and secure habitat, and most bears (78%) selected for secure habitat. Nonetheless, meanMCindex for secure habitat decreased over the study period during years of goodWBPproductivity. The wide diet breadth and foraging plasticity of grizzly bears likely allowed them to adjust to decliningWBP. Bears reduced use ofWBPstands without increasing movement rates, suggesting they obtained alternative fall foods within their local surroundings. However, the reduction in mortality risk historically associated with use of secure, high‐elevationWBPhabitat may be diminishing for bears residing in multiple‐use areas.
Asymmetric Diastereoselective Synthesis of Spirocyclopropane Derivatives of Oxindole
Authors: Ošeka Maksim , Noole Artur , Žari Sergei , Öeren Mario , Järving Ivar , Lopp Margus , Kanger Tõnis 
Published: April 2014

AbstractA new asymmetric organocatalytic synthesis of spirocyclopropane oxindoles has been developed. The method is based on the Michael addition of N‐Boc‐protected 3‐chlorooxindole to unsaturated 1,4‐dicarbonyl compounds, affording trans‐substituted spirocyclopropane oxindole derivatives in high diastereo‐ and enantioselectivity.
Spectrum trading algorithm based on memory in cognitive radio network
Authors: Zhang Shibing , Zhang Guodong , Bao Zhihua , Zhang Xiaoge 
Published: April 2014

AbstractIn cognitive radio networks, the most important goal of spectrum sharing is to benefit both the seller and buyer. In order to increase the total benefits generated in spectrum sharing, this paper introduces a memory mechanism between the spectrum agent and multi‐primary service providers (PSPs) and proposes a spectrum trading algorithm based on the mechanism. In this algorithm, all PSPs compete with each other and sell their idle spectrum resource to the agent to maximise their own profits. Then, the agent auctions the spectrum resources obtained in multi‐secondary users. Finally, a new auction pricing function is designed to decrease the auction trading prices and increase the benefits of secondary users. Nash equilibrium is considered to be the optimal result. Simulation results show that the proposed algorithm provides 10–15% margin over the conventional algorithm both in the price and spectrum quantity. Copyright © 2014 John Wiley & Sons, Ltd.
Interference management in LTE‐based HetNets: a practical approach
Authors: Font‐Bach O. , Bartzoudis N. , Pascual‐Iserte A. , Payaró M. , Blanco L. , López Bueno D. , Molina M. 
Published: April 2014

AbstractInterference is a major obstacle in radio communications, especially when opportunistic frequency reuse is an inherent requirement for maximising spectral efficiency in heterogeneous networks. A typical example is encountered in cellular communications where macrocell‐edge users receive interference from small cell transmissions that use the same radio frequency band. Innovating interference management algorithms are employed towards this end, which because of their interdependencies with numerous parameters of the target operating scenario and various low‐level implementation aspects, need to be prototyped in real‐time signal processing platforms in order to be credibly verified. In this paper, we present the development and experimental validation of a macrocell/femtocell coexistence scenario in close to real‐life conditions. The inclusion of an agile interference management scheme increased the signal processing complexity at the physical layer. This overhead was appropriately addressed by engaging advanced parallel processing techniques, optimisations of the arithmetic operations and intelligent reuse of logic and memory resources in the field programmable gate array (FPGA)‐based baseband processing architecture. Copyright © 2014 John Wiley & Sons, Ltd.