bluuebunny/crossref_metadata_2025_split huggingface dataset:
Destabilization of Egg Yolk Emulsion After IgY Removal Through Enzymatic Treatments
Authors: Navidghasemizad Sahar , Acero‐Lopez Alexandra , Curtis Jonathan , Temelli Feral , Wu Jianping 
Published: September 2014

AbstractThe objective of this study was to destabilize the protein–lipid complex in egg yolk precipitate obtained after the removal of soluble proteins, referred to as the pellet, through enzymatic treatment for further phospholipids extraction. A combination of proteolytic and lipolytic enzymes was applied to release the lipids from the pellet or weaken the pellet emulsion. Emulsions prepared using Protease P/Lipase AY30, Protease II/Lipase AY30 and Protease M/Lipase AY30 treated pellets had larger oil droplets (78, 65, 56 µm) and higher coalescence rates (51, 41, 35 %) than those of Protex 51FP, pellet, Protex 7L and Protease A with oil droplet size of 20, 18, 15 and 13 µm and coalescence rates of 31, 8, 7.5 and 8 %, respectively. Cream and liquid subnatant fractions obtained after further centrifugation of hydrolysates were subjected to lipid analyses. Over 90 % of phosphatidylcholine (PC) present in the pellet and 80 % of that in the original egg yolk were recovered in the cream from Protease P/Lipase AY30 treatment, while the recovery of PC from the egg yolk was significantly lower in creams from Protex 7L or Protease 51FP treatments (12 and 10 %, respectively). Pellets treated with Protease M, Protex 7L or Protex 51FP in combination with Lipase AY30 led to a significant loss of PC due to the conversion of PC to lysophosphatidylcholine or its degradation. Cream fractions obtained from the study represented a better material for the recovery of PL than intact egg yolk using environmentally‐friendly techniques such as supercritical carbon dioxide (SC‐CO2) extraction.
Impaired VLDL assembly: a novel mechanism contributing to hepatic lipid accumulation following ovariectomy and high-fat/high-cholesterol diets?
Authors: Côté Isabelle , Chapados Natalie A. , Lavoie Jean-Marc 
Published: September 2014

The aim of the present study was to identify molecular mechanisms involved in liver fat and cholesterol accumulation in ovariectomised (Ovx) rats fed with high-cholesterol diets. VLDL assembly and bile acid metabolism were specifically targeted. After being either Ovx or sham-operated, the rats were fed a standard diet or a high-fat diet containing 0, 0·25 or 0·5 % cholesterol for 6 weeks. Although Ovx rats exposed to dietary cholesterol intake accumulated the greatest amount of hepatic fat and cholesterol, plasma cholesterol levels were lower (P< 0·05) in these animals than in the corresponding control rats. Accompanying this observation, ovariectomy and dietary cholesterol intake resulted in a down-regulation (P< 0·05) of the expression of genes associated with VLDL assembly, including microsomal TAG transfer protein, diacylglycerol acyltransferase 2, acyl-CoA:cholesterol acyltransferase 2 and apoB-100 as well as genes associated with bile acid metabolism including farnesoid X receptor and bile salt export pump (P< 0·01). These results indicate that high-fat/high-cholesterol diets and ovariectomy concomitantly disrupt hepatic lipid output through defects in VLDL assembly and, most probably, secretion. The results also point to a defect in hepatic bile acid secretion. The present study offers novel insights into intrahepatic lipid metabolism, which may be relevant to metabolic complications found in postmenopausal women.
Allophanic and ferric root-associated stalactites: biomineralization induced by microbial activity (Galeria da Queimada lava tube, Terceira, Azores)
Authors: DAZA R. , BUSTILLO M. A. 
Published: September 2014

AbstractRoot-associated stalactites (rootsicles) in Galeria da Queimada lava tube have a mineralogical composition and developmental association with microbes that render them unique. Samples were examined by X-ray diffraction, micro-Raman spectrometry and scanning electron microscopy/X-ray energy-dispersive spectroscopy. Three types of rootsicle were defined: incipient; hard (white and red); and black spongy. The incipient rootsicles still contained rotten organic material and showed the beginning of mineralization by allophane. The white hard and black spongy types were also composed of allophane, while the red hard type was composed of hydrous ferric oxi-hydroxide minerals (HFO). The allophane and HFO in the andisol covering the cave roof precipitated out of the dripwater running along the roots to form the studied rootsicles. All three types of rootsicle showed black layers, coatings, spots or patches composed of manganese oxide minerals and, occasionally, hisingerite (iron (III) phyllosilicate). An alternation of organic precipitation caused by filamentous bacteria and inorganic precipitation (the latter facilitated by pH changes in the dripwater and the cave's temperature) built up both the porous and compact rings observed in the white and red hard rootsicles. The largely straight filaments seen in the porous rings of the white hard rootsicles may be indicative of the previous presence ofLeptothrixspp., while the helical morphologies seen in the red hard rootsicles may be indicative of that ofGallionellaspp. The manganese oxide minerals detected probably formed via microbial activity. This study reflects the important role of filamentous bacteria in rootsicle formation, independent of their mineralogy.
Staphylococcus aureusreservoirs during traditional Austrian raw milk cheese production
Authors: Walcher Georg , Gonano Monika , Kümmel Judith , Barker Gary C. , Lebl Karin , Bereuter Othmar , Ehling-Schulz Monika , Wagner Martin , Stessl Beatrix 
Published: September 2014

Sampling approaches following the dairy chain, including microbiological hygiene status of critical processing steps and physicochemical parameters, contribute to our understanding of howStaphylococcus aureuscontamination risks can be minimised. Such a sampling approach was adopted in this study, together with rapid culture-independent quantification ofStaph. aureusto supplement standard microbiological methods. A regional cheese production chain, involving 18 farms, was sampled on two separate occasions. Overall, 51·4% of bulk milk samples were found to beStaph. aureuspositive, most of them (34·3%) at the limit of culture-based detection.Staph. aureuspositive samples >100 cfu/ml were recorded in 17·1% of bulk milk samples collected mainly during the sampling in November. A higher number ofStaph. aureuspositive bulk milk samples (94·3%) were detected after applying the culture-independent approach. A concentration effect ofStaph. aureuswas observed during curd processing.Staph. aureuswere not consistently detectable with cultural methods during the late ripening phase, but >100Staph. aureuscell equivalents (CE)/ml or g were quantifiable by the culture-independent approach until the end of ripening. Enterotoxin gene PCR and pulsed-field gel electrophoresis (PFGE) typing provided evidence that livestock adapted strains ofStaph. aureusmostly dominate the post processing level and substantiates the belief that animal hygiene plays a pivotal role in minimising the risk ofStaph. aureusassociated contamination in cheese making. Therefore, the actual data strongly support the need for additional sampling activities and recording of physicochemical parameters during semi-hard cheese-making and cheese ripening, to estimate the risk ofStaph. aureuscontamination before consumption.
Doha stalemate: The end of trade multilateralism?
Authors: MUZAKA VALBONA , BISHOP MATTHEW LOUIS 
Published: September 2014

AbstractThis article challenges conventional narratives that suggest that the travails in the Doha Round, the shift to bilateral free trade agreements, and the broader unfolding of the global crisis collectively presage the decline of either the WTO or the broader institution of multilateral trade. We question the extent to which recent trends can indeed be said to constitute a genuine crisis of trade multilateralism by reflecting upon the contradictory and ambiguous nature of the multilateralism of the past, and also upon how contemporary multilateralism has been framed with reference to it. Our main finding is that, in contrast to the many short and medium-term symptoms which tend to appear in the conventional story of multilateral decline, there is actually a far more worrying long-term trend which underpins the varied conflicts that characterise contemporary trade politics: the fundamental lack of a shared social purpose between the developed countries and the more powerful emerging countries on which a stable, equitable, and legitimate edifice of multilateral trade rules can be erected, institutionalised, and enhanced.
Brain Functional Correlates of Working Memory: Reduced Load-Modulated Activation and Deactivation in Aging without Hyperactivation or Functional Reorganization
Authors: Kaup Allison R. , Drummond Sean P.A. , Eyler Lisa T. 
Published: September 2014

AbstractWe aimed to identify brain functional correlates of working memory performance in aging, in hopes of facilitating understanding of mechanisms that promote better versus worse working memory in late-life. Among 64 healthy adults, aged 23 to 78, we examined the relationship between age, working memory performance, and brain functional response during task performance. We focused on the association between working memory load-modulated functional response and individual differences in performance and whether these function-performance relationships differed with age. As expected, older age was associated with poorer working memory performance. Older age was also associated with reduced load-modulated activation including in bilateral prefrontal and parietal regions and left caudate as well as reduced deactivation including in the medial prefrontal cortex. Contrary to findings of hyperactivation in aging, we found no evidence of increased activation with older age. Positive associations identified between brain response and performance did not differ with age. Our findings suggest that the neural mechanisms underlying better versus worse working memory performance are age-invariant across adulthood, and argue against a pattern of functional reorganization in aging. Results are discussed within the broader literature, in which significant heterogeneity in findings between studies has been common. (JINS, 2014, 20, 1–6)
A Sufi Saint in Sixteenth-Century East Turkistan: New Evidence Concerning the Life of Khwāja Isḥāq
Authors: EDEN JEFF 
Published: September 2014

AbstractThe rise of Khwāja Isḥāq, one of the most influential figures in the history of Central Asian Sufism, has often been explained by the patronage of particular royals. This “royal patron” model has, however, been based on a flawed reading of hagiographical sources by which crucial questions of genre are overlooked in the effort of mining these complex narratives for apparent historical facts. New evidence, presented here, allows us to question the “royal patron” model, along with the commonly-accepted chronology of Khwāja Isḥāq's life, and calls for a different approach to Sufi hagiography as a historical source.
Prevalence of hyperthyroidism according to type of vegetarian diet
Authors: Tonstad Serena , Nathan Edward , Oda Keiji , Fraser Gary E 
Published: September 2014

AbstractObjectiveVegetarian diets may be associated with low prevalence of autoimmune disease, as observed in rural sub-Saharan Africans. Graves’ disease, an autoimmune disorder, is the most common cause of hyperthyroidism. We studied prevalence of hyperthyroidism according to dietary pattern in a population with a high proportion of vegetarians.DesignCross-sectional prevalence study. The association between diet and prevalence of hyperthyroidism was examined using multivariate logistic regression analyses controlling for sociodemographic characteristics and salt use.SettingThe Adventist Health Study-2 conducted in the USA and Canada.SubjectsChurch members (n 65 981) provided demographic, dietary, lifestyle and medical history data by questionnaire.ResultsThe prevalence of self-reported hyperthyroidism was 0·9 %. Male gender (OR=0·32; 95 % CI 0·26, 0·41) and moderate or high income (OR=0·67; 95 % CI 0·52, 0·88 and OR=0·73; 95 % CI 0·58, 0·91, respectively) protected against hyperthyroidism, while obesity and prevalent CVD were associated with increased risk (OR=1·25; 95 % CI 1·02, 1·54 and OR=1·92; 95 % CI 1·53, 2·42, respectively). Vegan, lacto-ovo and pesco vegetarian diets were associated with lower risk compared with omnivorous diets (OR=0·49; 95 % CI 0·33, OR=0·72, 0·65; 95 % CI 0·53, 0·81 and OR=0·74; 95 % CI 0·56, 1·00, respectively).ConclusionsExclusion of all animal foods was associated with half the prevalence of hyperthyroidism compared with omnivorous diets. Lacto-ovo and pesco vegetarian diets were associated with intermediate protection. Further study of potential mechanisms is warranted.
XTACC3–XMAP215 association reveals an asymmetric interaction promoting microtubule elongation
Authors: Mortuza Gulnahar B. , Cavazza Tommaso , Garcia-Mayoral Maria Flor , Hermida Dario , Peset Isabel , Pedrero Juan G. , Merino Nekane , Blanco Francisco J. , Lyngsø Jeppe , Bruix Marta , Pedersen Jan Skov , Vernos Isabelle , Montoya Guillermo 
Published: September 2014

AbstractchTOG is a conserved microtubule polymerase that catalyses the addition of tubulin dimers to promote microtubule growth. chTOG interacts with TACC3, a member of the transforming acidic coiled-coil (TACC) family. Here we analyse their association using the Xenopus homologues, XTACC3 (TACC3) and XMAP215 (chTOG), dissecting the mechanism by which their interaction promotes microtubule elongation during spindle assembly. Using SAXS, we show that the TACC domain (TD) is an elongated structure that mediates the interaction with the C terminus of XMAP215. Our data suggest that one TD and two XMAP215 molecules associate to form a four-helix coiled-coil complex. A hybrid methods approach was used to define the precise regions of the TACC heptad repeat and the XMAP215 C terminus required for assembly and functioning of the complex. We show that XTACC3 can induce the recruitment of larger amounts of XMAP215 by increasing its local concentration, thereby promoting efficient microtubule elongation during mitosis.
Genomes of diverse isolates of the marine cyanobacterium Prochlorococcus
Authors: Biller Steven J. , Berube Paul M. , Berta-Thompson Jessie W. , Kelly Libusha , Roggensack Sara E. , Awad Lana , Roache-Johnson Kathryn H. , Ding Huiming , Giovannoni Stephen J. , Rocap Gabrielle , Moore Lisa R. , Chisholm Sallie W. 
Published: September 2014

AbstractThe marine cyanobacterium Prochlorococcus is the numerically dominant photosynthetic organism in the oligotrophic oceans, and a model system in marine microbial ecology. Here we report 27 new whole genome sequences (2 complete and closed; 25 of draft quality) of cultured isolates, representing five major phylogenetic clades of Prochlorococcus. The sequenced strains were isolated from diverse regions of the oceans, facilitating studies of the drivers of microbial diversity—both in the lab and in the field. To improve the utility of these genomes for comparative genomics, we also define pre-computed clusters of orthologous groups of proteins (COGs), indicating how genes are distributed among these and other publicly available Prochlorococcus genomes. These data represent a significant expansion of Prochlorococcus reference genomes that are useful for numerous applications in microbial ecology, evolution and oceanography.
The angiogenic asset of soft tissue sarcomas: a new tool to discover new therapeutic targets
Authors: Rocchi Laura , Caraffi Stefano , Perris Roberto , Mangieri Domenica 
Published: September 2014

STS (soft tissue sarcomas) are rare malignant tumours deriving from cells of mesenchymal origin and represent only 1% of all malignant neoplasms. It has been extensively demonstrated that angiogenesis has an important role in cancer malignancy. Particularly, a lot of studies demonstrate the importance of angiogenesis in the development of carcinomas, whereas little is known about the role of angiogenesis in sarcomas and especially in STS. This review aims at summarizing the new discoveries about the nature and the importance of angiogenesis in STS and the new possible therapeutic strategies involved. Only a few studies concerning STS focus on tumour neovascularization and proangiogenic factors and look for a correlation with the patients prognosis/survival. These studies demonstrate that intratumoural MVD (microvessels density) may not accurately represent the angiogenic capacity of STS. Nevertheless, this does not exclude the possibility that angiogenesis could be important in STS. The importance of neoangiogenesis in soft tissue tumours is confirmed by the arising number of publications comparing angiogenesis mediators with clinical features of patients with STS. The efficacy of anti-angiogenic therapies in other types of cancer is well documented. The understanding of the involvement of the angiogenic process in STS, together with the necessity to improve the therapy for this often mortal condition, prompted the exploration of anti-tumour compounds targeting this pathway. In conclusion, this review emphasizes the importance to better understand the mechanisms of angiogenesis in STS in order to subsequently design-specific target therapies for this group of poorly responding tumours.
Determinants of insurance adoption among Bulgarian farmers
Authors: Lefebvre Marianne , Nikolov Dimitre , Gomez-y-Paloma Sergio , Chopeva Minka 
Published: September 2014

Purpose
– The purpose of this paper is to analyze the determinants of agricultural insurance adoption in Bulgaria, using a purpose-built survey of 224 farmers interviewed in 2011. The insurance decision is analyzed conjointly with other risk management decisions on the farm such as having contracts with retailers or processors, diversifying farm activities and using irrigation.

Design/methodology/approach
– The agricultural insurance sector in Bulgaria is presented in the broader context of the transition to a market-oriented economy and integration of Bulgarian agriculture into the EU Common Agricultural Policy. The recent developments on the determinants of farm insurance adoption in the agricultural economics and finance literature are discussed. A multivariate probit model is used in order to determine the factors explaining the adoption or non-adoption of various risk management tools by the surveyed farmers, including farm insurance.

Findings
– The authors find that farmers with diversified activities, using irrigation or having contracts with retailers or processors, are more likely to adopt insurance, after controlling for farms and farmers’ structural characteristics. Additionally, the authors find that the main characteristics distinguishing farmers who purchase agricultural insurance from non-users are farm size and farm location. The existence of strong regional effect suggests the importance of adapting the insurance products to the different regional contexts in Bulgaria.

Originality/value
– This paper contributes to the (limited) literature on agricultural insurance adoption in transition countries, currently shifting from a system where compensation against natural hazards tended to come from a State damage mitigation fund, inherited from the centrally planned governments to private and voluntary agricultural insurance. This research provides a unique data source on the Bulgarian case study.
Quality performance of SMEs in a developing economy: direct and indirect effects of service innovation and entrepreneurial orientation
Authors: Oly Ndubisi Nelson , Agarwal James 
Published: September 2014

Purpose– The purpose of this paper is to examine how innovation and entrepreneurial orientation (EO) affect organizational performance on quality in Asian small enterprise context.Design/methodology/approach– Drawing from the strategic management literature, we hypothesize and test the direct and indirect relationship between EO, innovation and quality performance in the context of small information technology (IT) firms in an Asian economy. Data analyses follow standard procedures for testing direct and mediating effects.Findings– Findings indicate a significant direct and indirect positive relationship between EO dimensions and three types of innovation and quality performance. Innovation mediates in the relationship of EO with quality performance.Research limitations/implications– The paper adds resource-based view and dynamic capabilities theories to extant strategic management literature. Poor representation of women-owned small firms in the study resulting from low participation of females in the IT business sector is a limitation which needs to be addressed in the future, as it hinders a clearer understanding of the perspectives of women business owners.Practical implications– The paper contributes to managerial practice by underscoring the need for owner-managers of small enterprises to pursue EO-focused and innovation enhancement strategies in an integrated manner.Originality/value– An integrated model of EO, innovation and performance, tested in small IT service firms in the context of a developing economy. Context does matter. The combination of a developing country context and the significance of IT enhance the contextual contribution of the paper.
A multi-level exploration of empowerment mediators
Authors: Anne Ritter Barbara , Venkatraman Srinivasan , Schlauch Carrie 
Published: September 2014

Purpose– The purpose of this paper is to empirically explore the underlying mechanisms through which empowerment affects organizational outcomes via a multi-dimensional model. Specifically, the paper suggests that empowerment climate (EC) is positively related to psychological empowerment (PE) and the effect of PE on the outcome variable of job satisfaction is mediated by justice perceptions and role clarity.Design/methodology/approach– In total, 765 employees at the executive level across 26 distinct groups in a large manufacturing firm participated in the survey. Multi-level analyses based on both hierarchical linear modeling and multi-level structural equation modeling techniques were utilized to test the hypotheses.Findings– This research demonstrated that EC significantly affected PE and that perceptions of justice and role clarity mediated the relationship between PE and job satisfaction. Additional analyses demonstrated that EC did not significantly affect organizational outcomes above and beyond PE, justice, and role clarity.Practical implications– The current study suggests that managers concerned with increasing employee perceptions of justice may be able to do so by increasing perceptions of employee empowerment. As only individual employee perceptions of empowerment related directly to organizational outcomes, this demonstrates the importance for managers to understand perceptions of employees.Originality/value– Exploring these mechanisms will strengthen the knowledge regarding how empowerment works to enhance organizational effectiveness. This will enable practitioners to better determine how and when empowerment will be most effective.