bluuebunny/crossref_metadata_2025_split huggingface dataset:
Composite cryogels for lysozyme purification
Authors: Baydemir Gözde , Türkoğlu Emir Alper , Andaç Müge , Perçin Işık , Denizli Adil 
Published: June 2014

AbstractBeads‐embedded novel composite cryogel was synthesized to purify lysozyme (Lyz) from chicken egg white. The poly(hydroxyethyl methacrylate‐N‐methacryloyl‐L‐phenylalanine) (PHEMAPA) beads of smaller than 5 µm size were synthesized by suspension polymerization and then embedded into a poly(hydroxyethyl methacrylate) (PHEMA)‐based cryogel column. The PHEMAPA bead‐embedded cryogel (BEC) column was characterized by swelling tests, scanning electron microscopy (SEM), surface area measurements by the Brunauer–Emmett–Teller (BET) method, elemental analysis, and flow dynamics. The specific surface area of the PHEMAPA BEC was found as 41.2 m2/g using BET measurements. Lyz‐binding experiments were performed using aqueous solutions in different conditions such as initial Lyz concentration, pH, flow rate, temperature, and NaCl concentration of an aqueous medium. The PHEMAPA BEC column could be used after 10 adsorption–desorption studies without any significant loss in adsorption capacity of Lyz. The PHEMAPA BEC column was used to purify Lyz from chicken egg white, and gel electrophoresis was used to estimate the purity of Lyz. The chromatographic application of the PHEMAPA BEC column was also performed using fast protein liquid chromatography.
UV fluorescence of tryptophan residues effectively measures protein binding to nucleic acid fragments immobilized in gel elements of microarrays
Authors: Zasedateleva Olga A. , Vasiliskov Vadim A. , Surzhikov Sergey A. , Sazykin Alexey Y. , Putlyaeva Lidiya V. , Schwarz Anton M. , Kuprash Dmitry V. , Rubina Alla Y. , Barsky Victor E. , Zasedatelev Alexander S. 
Published: June 2014

AbstractMicroarrays allow for the simultaneous monitoring of protein interactions with different nucleic acid (NA) sequences immobilized in microarray elements. Either fluorescently labeled proteins or specific fluorescently labeled antibodies are used to study protein–NA complexes. We suggest that protein–NA interactions on microarrays can be analyzed by ultraviolet (UV) fluorescence of tryptophan residues in the studied proteins, and this approach may eliminate the protein‐labeling step. A specialized UV microscope was developed to obtain fluorescent images of microarrays in the UV wavelengths and to measure the fluorescence intensity of individual microarray elements. UV fluorescence intensity of BSA immobilized in microarray gel elements increased linearly with increased BSA amount with sensitivity of 0.6 ng. Real‐time interaction curves between the DNA‐binding domain of the NFATc1 transcription factor (NFATc1‐DBD) and synthetic hairpin‐forming oligodeoxyribonucleotides immobilized within 0.2 nL microarray gel elements at a concentration 5 × 10–5 M and higher were obtained. The UV fluorescence intensities of microarray gel elements containing NFATc1‐DBD–DNA complexes at equilibrium allowed the estimation of the equilibrium binding constant for complex formation. The developed method allows the protein–NA binding to be monitored in real time and can be applied to assess the sequence‐specific affinity of NA‐binding proteins in parallel studies involving many NA sequences.
Oxidative refolding of rPA in l‐ArgHCl and in ionic liquids: A correlation between hydrophobicity, salt effects, and refolding yield
Authors: Tischer Alexander , Lilie Hauke , Auton Matthew , Lange Christian 
Published: June 2014

ABSTRACTThe ionic liquid 1‐ethyl‐3‐methyl imidazolium chloride (EMIM Cl) and the amino acid l‐arginine hydrochloride (l‐ArgHCl) have been successfully used to improve the yield of oxidative refolding for various proteins. However, the molecular mechanisms behind the actions of such solvent additives—especially of ionic liquids—are still not well understood. To analyze these mechanisms, we have determined the transfer free energies from water into ionic liquid solutions of proteinogenic amino acids and of diketopiperazine as peptide bond analogue. For EMIM Cl and 1‐ethyl‐3‐methyl imidazolium diethyl phosphate, which had a suppressive effect on protein refolding, as well as for l‐ArgHCl favorable interactions with amino acid side chains, but no favorable interactions with the peptide backbone could be observed. A quantitative analysis of other ionic liquids together with their already published effects on protein refolding showed that only solvent additives within a certain range of hydrophobicity, chaotropicity and kosmotropicity were effective for the refolding of recombinant plasminogen activator. © 2014 Wiley Periodicals, Inc. Biopolymers 101: 1129–1140, 2014.
Development and validation of an LC‐MS/MS method for the determination of mesalazine in beagle dog plasma and its application to a pharmacokinetic study
Authors: Qin Juan , Di Xin , Wang Xin , Liu Youping 
Published: June 2014

AbstractA simple, specific and sensitive LC‐MS/MS method was developed and validated for the determination of mesalazine in beagle dog plasma. The plasma samples were prepared by protein precipitation, then the separation of the analyte was achieved on a Waters Spherisorb C6 column (150 × 4.6 mm, 5 µm) with a mobile phase consisting of 0.2% formic acid in water–methanol (20:80, v/v). The flow rate was set at 1.0 mL/min with a split ratio of 3:2. Mass spectrometric detection was achieved by a triple‐quadrupole mass spectrometer equipped with an electrospray source interface in positive ionization mode. Quantitation was performed using selected reaction monitoring of precursor–product ion transitions at m/z 154 → m/z 108 for mesalazine and m/z 285 → m/z 193 for diazepam (internal standard). The linear calibration curve of mesalazine was obtained over the concentration range 50–30,000 ng/mL. The matrix effect of mesalazine was within ±9.8%. The intra‐ and inter‐day precisions were <7.9% and the accuracy (relative error) was within ±3.5%. The validated method was successfully applied to investigate the pharmacokinetics of mesalazine in healthy beagle dogs after rectal administration of mesalazine suppository. Copyright © 2014 John Wiley & Sons, Ltd.
Modulating the Helicity of Sugar‐Substituted Perylene Diimide Self‐assemblies by Solvent Polarilities
Authors: Guo Qiang , Wang Junchao , Zhu Lingyun , Wei Zhixiang 
Published: June 2014

AbstractModulating the helicity of self‐assemblies of conjugated molecules is important for their application in chiral electronics. In this communication, solvent effect has been investigated on how to modulate the helicity of supramolecular assemblies of sugar‐substituted perylenediimide (PTCDI‐BAG) by using UV‐Vis, circular dichroism spectroscopy and transmission electron microscopy. Left‐handed helical nanowires are obtained in the mixed solvent system of both N,N‐dimethylformamide/ethanol (DMF/ethanol) and N,N‐dimethylacetamide/ethanol (DMAC/ethanol). As changing the solvents into dimethyl sulfoxide/ethanol (DMSO/ethanol), ethylene glycol/ethanol (EG/ethanol), or N‐methyl‐2‐pyrrolidone/ethanol (NMP/ethanol), only right‐handed heical nanowires are observed. The chirality of supramolecular structures can be tuned by the controlling of the polarity of the solvents. A theoretical calculation is carried out to explain the chiral optical inversion phenomena of PTCDI‐BAG aggregates in different solvents, which reveals that reversed CD spectra is due to the difference of clockwise and anticlockwise rotation of the dimer.
Design, Synthesis, Structure‐Activity Relationship and Insecticidal Activities of Trifluoromethyl‐Containing Sulfiliminyl and Sulfoximinyl Phthalic Acid Diamide Structure
Authors: Zhou Sha , Yan Tao , Zhou Sha , Hua Xuewen , Wang Baolei , Gu Yucheng , Xiong Lixia , Li Yongqiang , Li Zhengming 
Published: June 2014

AbstractDue to a novel mode action, low toxicity to mammals and low residue characteristics, phthalic acid diamides have aroused considerable interests in agricultural chemistry. With introduction of N‐cyano, N‐trifluoroacetyl, N‐carbamoylsulfiliminyl and sulfoximinyl substituents into phthalamides, 12 novel derivatives containing trifluoromethyl moiety were designed and synthesized. All title compounds were characterized by 1H NMR and high‐resolution mass spectrometry. The preliminary results of biological activity assessment indicated that some title compounds exhibited moderate to good insecticidal activities against oriental armyworm (Pseudaletia separata Walker). In particular, Va gave higher activity against oriental armyworm and diamondback moth. The present work reported that the new trifluoromethylated diamides incorporating N‐trifluoroacetylsulfoximinyl moieties are potential lead compounds for further structure optimization, providing some insight into the relating structure‐activity relationship.
Synthesis, Structure and Properties of an Unusual 2D Network Zinc Coordination Polymer Based on Pentanuclear Zinc Cluster
Authors: Ding Jiangang , Yin Chao , Li Ke , Li Baolong , Wu Bing 
Published: June 2014

AbstractAn unusual zinc(II) coordination polymer [Zn5(OH)2(bbtz)2(bdc)4]n (1) [bbtz1,4‐bis(1,2,4‐triazol‐1‐ylmethyl)benzene, bdc1,2‐benzenedicarboxylate] was synthesized and characterized. 1 contains an unusual 2D network based on pentanuclear zinc(II) cluster [Zn5(OH)2]. Two adjacent pentanuclear Zn(II) clusters are bridged by four bdc ligands and extend to form the one‐dimensional chain. The bbtz ligands further link the one‐dimensional chains, resulting in a two‐dimensional coordination network. The luminescence and thermal stability were investigated.
Straightforward Synthesis of Various 2,3‐Diarylimidazo[1,2‐a]pyridines in PEG400 Medium through One‐Pot Condensation and C–H Arylation
Authors: Hiebel Marie‐Aude , Fall Yacoub , Scherrmann Marie‐Christine , Berteina‐Raboin Sabine 
Published: June 2014

AbstractPEG400 is described herein as a suitable medium for the condensation of various 2‐amino pyridines with α‐bromo ketones. 2‐Arylimidazo[1,2‐a]pyridines were synthetized in a short time through microwave irradiation in moderate to excellent yields. After optimization, a convenient one‐pot process enabled access to 2,3‐diarylimidazo[1,2‐a]pyridines by using a reduced amount of palladium catalyst without ligand for the C–H arylation step in the same environmentally‐sound medium.
Same task, different strategies: How brain networks can be influenced by memory strategy
Authors: Sanfratello Lori , Caprihan Arvind , Stephen Julia M. , Knoefel Janice E. , Adair John C. , Qualls Clifford , Lundy S. Laura , Aine Cheryl J. 
Published: June 2014

AbstractPrevious functional neuroimaging studies demonstrated that different neural networks underlie different types of cognitive processing by engaging participants in particular tasks, such as verbal or spatial working memory (WM) tasks. However, we report here that even when a WM task is defined as verbal or spatial, different types of memory strategies may be used to complete it, with concomitant variations in brain activity. We developed a questionnaire to characterize the type of strategy used by individual members in a group of 28 young healthy participants (18–25 years) during a spatial WM task. A cluster analysis was performed to differentiate groups. We acquired functional magnetoencephalography and structural diffusion tensor imaging measures to characterize the brain networks associated with the use of different strategies. We found two types of strategies were used during the spatial WM task, a visuospatial and a verbal strategy, and brain regions and time courses of activation differed between participants who used each. Task performance also varied by type of strategy used with verbal strategies showing an advantage. In addition, performance on neuropsychological tests (indices from Wechsler Adult Intelligence Scale‐IV, Rey Complex Figure Test) correlated significantly with fractional anisotropy measures for the visuospatial strategy group in white matter tracts implicated in other WM and attention studies. We conclude that differences in memory strategy can have a pronounced effect on the locations and timing of brain activation and that these differences need further investigation as a possible confounding factor for studies using group averaging as a means for summarizing results. Hum Brain Mapp 35:5127–5140, 2014. © 2014 Wiley Periodicals, Inc.
Lack of a clinically important pharmacokinetic interaction between sofosbuvir or ledipasvir and hormonal oral contraceptives norgestimate/ethinyl estradiol in HCV‐Uninfected female subjects
Authors: German P. , Moorehead L. , Pang Phillip , Vimal M. , Mathias A. 
Published: June 2014

AbstractThis study evaluated the potential for a drug–drug interaction between HCV direct‐acting antivirals sofosbuvir or ledipasvir and oral hormonal contraceptive (OC) norgestimate/ethinyl estradiol (norgestimate 0.18/0.215/0.25 mg with ethinyl estradiol 25 μg). This was a 112‐day, open‐label, fixed‐sequence pharmacokinetic (PK) study in healthy female subjects that included a lead‐in cycle (OC only; N = 21), cycle 1 (OC only; N = 15), cycle 2 (OC + sofosbuvir; N = 15), and cycle 3 (OC + ledipasvir; N = 15). Administration of sofosbuvir with OC did not alter PK of norelgestromin (primary norgestimate metabolite) or ethinyl estradiol. Small increases in norgestrel (secondary norgestimate metabolite) AUCtau (19%) and Ctau (23%) with sofosbuvir were noted. Ledipasvir did not impact PK of norelgestromin or norgestrel but modestly increased ethinyl estradiol Cmax (40%). Sofosbuvir, GS‐ 331007 (predominant circulating metabolite of SOF), and ledipasvir PK were similar to historical data. Pharmacodynamic markers luteinizing hormone, follicle‐stimulating hormone, and progesterone values were generally comparable in all cycles. No loss in contraceptive efficacy is expected upon administration of sofosbuvir or ledipasvir/sofosbuvir with oral contraceptives containing norgestimate and ethinyl estradiol. The use of sofosbuvir or ledipasvir/sofosbuvir FDC with oral contraceptives is permitted.
Antibiotic prophylaxis for endoscopic retrograde chlangiopancreatography increases the detection rate of drug‐resistant bacteria in bile
Authors: Minami Tomoyuki , Sasaki Tamito , Serikawa Masahiro , Ishigaki Takashi , Murakami Yoshiaki , Chayama Kazuaki 
Published: June 2014

AbstractBackgroundNo consensus has yet been reached regarding the utility of antibiotic prophylaxis for endoscopic retrograde cholangiopancreatography (ERCP). However, there has been little discussion of potential adverse effects of antibiotic use. This study investigated the impact of antibiotic prophylaxis on overall levels of bacterial infiltration of the biliary tract and the prevalence of drug‐resistance among that population.MethodsNinety‐three patients, from whom intraoperative bile samples were collected after performing ERCP, were assigned to either an antibiotic‐prophylaxis group (AP, n = 58) or a no‐antibiotic‐prophylaxis group (NAP, n = 35). Detection rates of biliary bacteria and antibiotic resistance were determined for each group. Multivariate analysis was also performed to identify risk factors for the development of drug‐resistant biliary bacteria.ResultsThe bile contamination rate was 37.1% for the NAP group and 55.2% for the AP group (P = 0.09). Drug‐resistant bacteria were found in 5.7% of the NAP group and 29.3% of the AP group (P = 0.006). Biliary drainage and antibiotic prophylaxis for ERCP were identified as risk factors for the presence of drug‐resistant bacteria.ConclusionAdministration of antibiotic prophylaxis prior to ERCP can be a risk factor for the selection of drug‐resistant bacteria in the biliary tract.
Pharyngeal weakness and upper esophageal sphincter opening in patients with unilateral vocal fold immobility
Authors: Domer Amanda S. , Leonard Rebecca , Belafsky Peter C. 
Published: June 2014

Objectives/HypothesisTo evaluate pharyngeal strength and upper esophageal sphincter opening in patients with unilateral vocal fold immobility (UVFI).Study DesignCase control study.MethodsCharts of individuals with UVFI who underwent a videofluoroscopic swallow study were reviewed. To exclude confounding variables associated with pharyngeal weakness, inclusion was limited to patients with iatrogenic and idiopathic UVFI. Data abstracted included patient demographics, etiology of UVFI, pharyngeal constriction ratio (PCR), and upper esophageal sphincter (UES) opening (UESmax). Data were compared to age/gender‐matched controls with no history of dysphagia or UVFI. Discrete variables were analyzed using a chi‐square test of independence, and an independent samples t test was used to compare the UVFI and control groups (P = 0.05). A one‐way analysis of variance (ANOVA) was used to compare iatrogenic and idiopathic UVFI groups.ResultsThe mean age of the cohort (n = 25) was 61 (±14 SD) years and 52% was female. The etiologies of UVFI were iatrogenic (n = 17) and idiopathic (n = 8). Thirty‐eight percent of UVFI patients (n = 25) aspirated compared to 0% of controls (P < 0.05). The mean PCR for the UVFI group was 0.14 (±0.02) compared to 0.06 (±.01) for controls (P < 0.05). The mean UESmax for the UVFI group was 0.82 cm (±0.04) compared to 1.0 cm (±0.05) for controls (P > 0.05).ConclusionIndividuals with UVFI of iatrogenic and idiopathic etiologies with subjective dysphagia demonstrate objective evidence of pharyngeal weakness. The increased prevalence of aspiration in this population may not be solely the result of impaired airway protection.Level of Evidence3b. Laryngoscope 124:2371–2374, 2014
A traction‐based equilibrium finite element free from spurious kinematic modes for linear elasticity problems
Authors: Wang Li , Zhong Hongzhi 
Published: June 2014

SUMMARYThis paper presents equilibrium elements for dual analysis. A traction‐based equilibrium element is proposed in which tractions of an element instead of stresses are chosen as DOFs, and therefore, the interelement continuity and the Neumann boundary balance are directly satisfied. To be solvable, equilibrated tractions with respect to the space of rigid body motion are required for each element. As a result, spurious kinematic modes that may inflict troubles on stress‐based equilibrium elements do not appear in the element because only equilibrium constraints on tractions are required. An admissible stress field is eventually constructed in terms of the equilibrated tractions for the element, and hence, equilibrium finite element procedures can proceed. The element is also generalized to accommodate non‐zero body forces, nonlinear boundary tractions and curved Neumann boundaries. Numerical tests including a single equilibrium element, error estimation of a cantilever beam and an infinite plate with a circular hole are conducted, displaying excellent convergence and effectiveness of the element for error estimation. Copyright © 2014 John Wiley & Sons, Ltd.
Dietary self‐monitoring and long‐term success with weight management
Authors: Peterson Ninoska D. , Middleton Kathryn R. , Nackers Lisa M. , Medina Kristen E. , Milsom Vanessa A. , Perri Michael G. 
Published: June 2014

ObjectiveTo examine the contributions of frequency, consistency, and comprehensiveness of dietary self‐monitoring to long‐term weight change.MethodsParticipants included 220 obese women (mean ± SD, age = 59.3 ± 6.1 years; BMI = 36.8 ± 4.9 kg/m2) who achieved a mean weight loss of −10.39 ± 5.28% from baseline during 6 months of behavioral treatment and regained 2.30 ± 7.28% during a 12‐month extended‐care period. The contributions of cumulative frequency of self‐monitoring (total number of food records), consistency across time (number of weeks with ≥3 records), and comprehensiveness of information recorded were examined as predictors of weight regain in a hierarchical linear regression analysis. The mediating role of adherence to daily caloric intake goals was tested using a bootstrapping analysis.ResultsThe association between high total frequency of self‐monitoring and reduced weight regain was moderated by weekly consistency of self‐monitoring, P = 0.004; increased frequency produced beneficial effects on weight change only when coupled with high consistency (>3 days/week). There was no impact of comprehensiveness on weight change, P > 0.05. The favorable effect of high frequency/high consistency self‐monitoring on weight change was partially mediated by participants' success in meeting daily caloric intake goals (P < 0.001).ConclusionThe combination of high frequency plus high consistency of dietary self‐monitoring improves long‐term success in weight management.
Ring opening polymerization of epoxides with urea‐derivatives of 4‐aminopyridine as thermally latent anionic initiator
Authors: Makiuchi Naoyuki , Sudo Atsushi , Endo Takeshi 
Published: June 2014

ABSTRACTIn this study, a series of urea‐derivatives of 4‐aminopyridine (4AP) were evaluated as thermally latent initiators for the anionic ring‐opening polymerization of diglycidyl ether of bisphenol A (DGEBA). The urea‐derivatives were synthesized by the reactions of 4AP with the corresponding iso(thio)cyanates (phenyl isocyanate, tert‐butyl isocyanate, methylene diphenyl diisocyanate, and phenyl isothiocyanate). The ability of the urea‐derivatives as latent initiators was investigated with differential scanning calorimetry (DSC): Upon heating formulations comprised of DGEBA and the urea‐derivatives in a heating rate at 10 °C/min, the resulting DSC profiles indicated exothermic peaks to confirm that DGEBA underwent the polymerization efficiently. The corresponding DSC‐peak top temperatures (Tpeak  top) was higher than that observed for the formulation comprised of DGEBA and pristine 4AP, to clarify that the urea are useful initiators with thermal latency. A possible mechanism for the initiation step involves the thermal dissociation of the urea into 4AP and the corresponding isocyanates. 4AP thus generated readily initiated the ring‐opening polymerization of epoxide. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 2518–2522
Methyllycaconitine‐ and scopolamine‐induced cognitive dysfunction: differential reversal effect by cognition‐enhancing drugs
Authors: Andriambeloson Emile , Huyard Bertrand , Poiraud Etienne , Wagner Stéphanie 
Published: June 2014

AbstractThere is a growing body of evidence pointing to the pivotal role of alpha‐7 nicotinic acetylcholine receptor (α7 nAchR) dysfunction in cognitive disorders such as Alzheimer's disease or schizophrenia. This study was undertaken to establish and characterize an in vivo model for cognitive disorder secondary to the blockade of α7 nAChR by its specific antagonist, methyllycaconitine (MLA). The results show that MLA elicited cognitive dysfunction as assessed by reduced spontaneous alternation of mice in the T‐maze. The maximal effect of MLA produced 25–30% reduction in the spontaneous alternation of mice, a level comparable with that induced by the muscarinic antagonism of scopolamine. Donepezil and galantamine fully reversed both MLA and scopolamine‐induced cognitive dysfunction. However, the ED50 of donepezil and galantamine was significantly shifted to the left in the MLA‐ compared to scopolamine‐treated mice (0.0005 and 0.002 mg/kg for donepezil; 0.0003 and 0.7 mg/kg for galantamine). Moreover, memantine elicited marked reversion of cognitive dysfunction (up to 70%) in MLA‐treated mice while only a weak reversal effect at high dose of memantine (less than 20%) was observed in scopolamine‐treated mice. The above findings indicate that MLA‐induced cognitive dysfunction in the mouse is highly sensitive and more responsive to the current procognitive drugs than the traditional scopolamine‐based assay. Thus, it can be of value for the preclinical screening and profiling of cognition‐enhancing drugs.