bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution of cerebrospinal fluid sCD19 levels to the detection of CNS lymphoma and its impact on disease outcome
Authors: Muñiz Carmen , Martín-Martín Lourdes , López Antonio , Sánchez-González Blanca , Salar Antonio , Almeida Julia , Sancho Juan-Manuel , Ribera José María , Heras Cecilia , Peñalver Francisco Javier , Gómez Marta , González-Barca Eva , Alonso Natalia , Navarro Belén , Olave Teresa , Sala Francisco , Conde Eulogio , Márquez José Antonio , Cabezudo Elena , Cladera Antonia , García-Malo María , Caballero María Dolores , Orfao Alberto 
Published: February 2014

Key PointsIncreased levels of sCD19 protein in the CSF are associated with CNS disease in DLBCL and BL patients at risk of CNS lymphoma. Presence of lymphoma cells by FCM and/or increased CSF sCD19 levels are related with a poorer EFS and/or OS in DLBCL and BL patients.
Quantitative phosphoproteomics unveils temporal dynamics of thrombin signaling in human endothelial cells
Authors: van den Biggelaar Maartje , Hernández-Fernaud Juan Ramon , van den Eshof Bart L. , Neilson Lisa J. , Meijer Alexander B. , Mertens Koen , Zanivan Sara 
Published: February 2014

Key PointsThis is the first time-resolved quantitative phosphoproteomic analysis of thrombin signaling in human endothelial cells. We provide 2224 phosphosites regulated by thrombin as a unique resource for future studies on thrombin and PAR signaling.
VEGF-D-induced draining lymphatic enlargement and tumor lymphangiogenesis promote lymph node metastasis in a xenograft model of ovarian carcinoma
Authors: Du Li-Cheng , Chen Xian-Cheng , Wang Dong , Wen Yan-Jun , Wang Chun-Ting , Wang Xue-Mei , Kan Bing , Wei Yu-Quan , Zhao Xia 
Published: February 2014

Abstract

Background
Vascular endothelial growth factor (VEGF)-D has been shown to promote lymph node metastasis in several cancers. Although generally overexpressed in ovarian carcinoma, its role in nodal dissemination of this cancer is unclear. To clarify the role of VEGF-D and the underlying molecular mechanisms, we investigated the function of VEGF-D using a mouse xenograft model of ovarian cancer.

Methods
Human ovarian serous adenocarcinoma SKOV3 cells were transfected with VEGF-D recombinant plasmid DNA, or with control vectors. The cells were injected subcutaneously into the footpads of nude mice. Tumor growth was evaluated weekly. Draining lymphatics were observed grossly with Evan’s blue lymphangiography. Tumoral lymphatics were delineated with both Evan’s blue and LYVE-1 immunostaining. Tumor metastases to lymph nodes were evaluated by H&E and CA125/CD40 staining. Expression of VEGF-D in primary tumors and levels of CA125 in involved lymph nodes were examined by immunohistochemistry. Tumor cell apoptosis was analyzed by Hoechst dyeing.

Results
Mice bearing VEGF-D overexpressing xenografts showed a significantly higher rate of lymph node metastasis and markedly greater tumor volume compared with the controls. The functional lymphatic vessels were denser and enlarged in marginal and central tumor portions. Additionally, higher CA125 expression was observed in the involved lymph nodes. Mice bearing VEGF-D overexpressing xenografts also exhibited a markedly lower apoptotic index compared with the controls.

Conclusions
Our data demonstrate the important role of VEGF-D in promoting lymph node metastasis by increasing tumor lymphangiogenesis, stimulating draining lymphatic vessel formation, and enhancing tumor invasiveness. Our findings show that VEGF-D can be a promising therapeutic target for ovarian cancer.
Fast urinary screening of oligosaccharidoses by MALDI-TOF/TOF mass spectrometry
Authors: Bonesso Laurent , Piraud Monique , Caruba Céline , Van Obberghen Emmanuel , Mengual Raymond , Hinault Charlotte 
Published: February 2014

Abstract

Background
Oligosaccharidoses, which belong to the lysosomal storage diseases, are inherited metabolic disorders due to the absence or the loss of function of one of the enzymes involved in the catabolic pathway of glycoproteins and indirectly of glycosphingolipids. This enzymatic deficiency typically results in the abnormal accumulation of uncompletely degraded oligosaccharides in the urine. Since the clinical features of many of these disorders are not specific for a single enzyme deficiency, unambiguous screening is critical to limit the number of costly enzyme assays which otherwise must be performed.

Methods
Here we provide evidence for the advantages of using a MALDI-TOF/TOF (matrix-assisted laser desorption ionization time-of-flight) mass spectrometric (MS) method for screening oligosaccharidoses. Urine samples from previously diagnosed patients or from unaffected subjects were randomly divided into a training set and a blind testing set. Samples were directly analyzed without prior treatment.

Results
The characteristic MS and MS/MS molecular profiles obtained allowed us to identify fucosidosis, aspartylglucosaminuria, GM1 gangliosidosis, Sandhoff disease, α-mannosidosis, sialidosis and mucolipidoses type II and III.

Conclusions
This method, which is easily run in less than 30 minutes, is performed in a single step, and is sensitive and specific. Invaluable for clinical chemistry purposes this MALDI-TOF/TOF mass spectrometry procedure is semi-automatizable and suitable for the urinary screening of oligosacharidoses.
An exploration of parents' preferences for foot care in juvenile idiopathic arthritis: a possible role for the discrete choice experiment
Authors: Hendry Gordon J , Turner Debbie E , Gardner‐Medwin Janet , Lorgelly Paula K , Woodburn James 
Published: February 2014

AbstractBackgroundAn increased awareness of patients' and parents' care preferences regarding foot care is desirable from a clinical perspective as such information may be utilised to optimise care delivery. The aim of this study was to examine parents' preferences for, and valuations of foot care and foot‐related outcomes in juvenile idiopathic arthritis (JIA).MethodsA discrete choice experiment (DCE) incorporating willingness‐to‐pay (WTP) questions was conducted by surveying 42 parents of children with JIA who were enrolled in a randomised‐controlled trial of multidisciplinary foot care at a single UK paediatric rheumatology outpatients department. Attributes explored were: levels of pain; mobility; ability to perform activities of daily living (ADL); waiting time; referral route; and footwear. The DCE was administered at trial baseline. DCE data were analysed using a multinomial‐logit‐regression model to estimate preferences and relative importance of attributes of foot care. A stated‐preference WTP question was presented to estimate parents' monetary valuation of health and service improvements.ResultsEvery attribute in the DCE was statistically significant (p < 0.01) except that of cost (p = 0.118), suggesting that all attributes, except cost, have an impact on parents' preferences for foot care for their child. The magnitudes of the coefficients indicate that the strength of preference for each attribute was (in descending order): improved ability to perform ADL, reductions in foot pain, improved mobility, improved ability to wear desired footwear, multidisciplinary foot care route, and reduced waiting time. Parents' estimated mean annual WTP for a multidisciplinary foot care service was £1,119.05.ConclusionsIn terms of foot care service provision for children with JIA, parents appear to prefer improvements in health outcomes over non‐health outcomes and service process attributes. Cost was relatively less important than other attributes suggesting that it does not appear to impact on parents' preferences.
Radio with pictures: Radio visualization in BBC national radio
Authors: Berry Richard 
Published: February 2014

Abstract
It is popular to claim that radio has always had pictures – the ones listeners created in their own minds – and this is often seen as one of the medium’s greatest strengths. The process of visualization of radio in the digital age has most often focused on how producers can fill the screen of the various devices through which we receive radio today. This article focuses on the other ways in which radio is visualized online, in a process that deploys transmedia storytelling techniques. It looks at specific examples and explores the way in which these transmedia techniques build relationships with the audience, strengthen brands and create opportunities for broadcasters to tell stories in ways never before possible.
What attracts listeners to Web radio? A case study from Germany
Authors: Stark Birgit , Weichselbaum Philipp 
Published: February 2014

Abstract
With the advent of Web radio in a changing media environment, radio has once more reinvented itself in terms of both scope (emerging new forms such as online-only radio) and listening experience (interactivity, personalization and content modularization). Scholars have identified these technological changes as evidence for an evolving radio. The audience perspective, however, has been researched far less. We go beyond existing studies and are interested in the role signature features of Web radio play for its listeners. We answer two research questions: (1) What do users expect from listening to Web radio as an interactive medium? (2) What are the gratifications sought when tuning in to Web radio in comparison to offline radio? The research is based on an exploratory online survey among Web radio listeners in Germany. Our findings show that interactive Web radio features play only a minor role for listeners. Instead, they focus on music. When compared to other online media, listening to Web radio is ranked highest among affective gratification items. We discuss theoretical and practical implications of our findings, as well as Web radio’s potential impact on traditional radio.
Relevance of SGK1 in structural, functional and molecular alterations produced by aldosterone in heart
Authors: Martín-Fernández Beatriz , Muñoz María Valero , de las Heras Natalia , Ballesteros Sandra , Lahera Vicente 
Published: February 2014

Abstract
Aldosterone regulates sodium (Na+) and potassium (K+) transports in epithelial cells. Besides, aldosterone participates in cardiac alterations associated with hypertension, heart failure, diabetes, and other pathological alterations. One of the main cardiac alterations induced by aldosterone is cardiac hypertrophy in which different mechanisms are involved such as increased cardiomyocyte, calcium concentration, oxidative stress, and inflammatory and fibrotic mediators stimulation. Many epidemiological studies have demonstrated that left ventricular hypertrophy is associated with significantly increased risk of heart failure and malignant arrhythmias. SGK1 is a member of the serine/threonine kinase gene family that plays an important role in the absorption of Na+ and water through the Na+ channel in the apical membrane of tubular epithelial cells. SGK1 has been related to fibrotic mediator increase such as connective tissue growth factor (CTGF) and transforming growth factor-β (TGF-β) as well as inflammatory [tumor necrosis factor-α (TNF-α) and interleukin (IL)-1β] and oxidative (NADPH oxidase) species. It has been shown that aldosterone induces SGK1 gene expression not only in kidneys but also in the heart. Supporting the central role of SGK1 in cardiac alterations induced by aldosterone, treatment with the mineralocorticoid antagonist spironolactone is able to reduce the gene expression of SGK1 in aldosterone-treated rats. Taken together, data suggest the involvement of SGK1 in a complex intracellular signaling, involving fibrotic, inflammatory, and oxidative pathways, which lead to cardiac hypertrophy and fibrosis induced by aldosterone.
An Emotion Theory Approach to Artificial Emotion Systems for Robots and Intelligent Systems: Survey and Classification
Authors: Scheuring Sylvia Tidwell , Agah Arvin 
Published: February 2014

AbstractTo assist in the evaluation process when determining architectures for new robots and intelligent systems equipped with artificial emotions, it is beneficial to understand the systems that have been built previously. Other surveys have classified these systems on the basis of their technological features. In this survey paper, we present a classification system based on a model similar to that used in psychology and philosophy for theories of emotion. This makes possible a connection to thousands of years of discourse on the topic of emotion. Five theories of emotion are described based on an emotion theory model proposed by Power and Dalgleish. The paper provides classifications using a model of 10 new questions, for 14 major research projects that describe implementations or designs for systems that use artificial emotions for either robotics or general artificial intelligence. We also analyze the trends in the usage of various theories and complexity changes over time.
Echtzeitfähige Parameternachführung
zur modellbasierten Zustandsbestimmung automobiler
Abgasnachbehandlungssysteme
Authors: Schödel Sebastian , Fischerauer Gerhard 
Published: February 2014

Zusammenfassung
Moderne Verfahren zur Regelung von Ottomotoren berücksichtigen,
zusätzlich zu den gemessenen Luftzahlen im Abgas, den
Sauerstofffüllstand in Drei-Wege-Katalysatoren. Auf Grund dessen, dass
die Rechenleistung der im Fahrzeug verfügbaren Steuergeräte
eingeschränkt ist, werden für diese modellbasierte
Füllstandsschätzung stark vereinfachende Annahmen getroffen. Mit
Hilfe einer hochfrequenzbasierten Zustandsüberwachung, woraus sich der
Sauerstofffüllstand direkt bestimmen lässt, kann die Genauigkeit
bestehender Katalysatormodelle jedoch verbessert werden. Neben der
hochfrequenzbasierten Zustandsüberwachung stellen wir ein Verfahren vor,
welches die Parameter des Katalysatormodells im Betrieb nachführt, um
trotz alterungsbedingter Änderungen der Systemeigenschaften die
gewünschte Genauigkeit zu gewährleisten.
Abstandsensorsystem basierend auf dem Prinzip der optischen Reflektion
für Anwendungen im Bereich Weiße Ware: Intensitätsreferenzierung
und Linearisierung des Ausgnagssignals
Authors: Baumer Johannes , Shamonin Mikhail 
Published: February 2014

Zusammenfassung
Ein auf optischer Reflektion basierendes Abstandssensorsystem für die
„Weiße Ware“ wird hier vorgestellt. Die technische Lösung greift
auf ein allseits bekanntes Intensitätsreferenzierungsprinzip – die
optische Brücke – zurück. Der Einfluss der Umgebungstemperatur kann
kompensiert werden. Störeffekte wie alterungsbedingte Drift der
optischen Elemente oder Systemvariationen der Empfindlichkeit verursacht
durch die Streuung der einzelnen Komponenten können erfolgreich
gehandhabt werden. Ein Algorithmus zur Ausgangslinearisierung und dessen
Optimierung in Hinblick auf einen kostengünstigen Mikrokontroller wird
beschrieben. Zum Schluss wird ein Prototyp eines Fertigungsautomaten, der
zur Herstellung dieses Sensors dient, und dessen grundlegende Funktion
vorgestellt.
Nanostructured LB Films Developed from Ferrocenylthioamide and Ferrocenylselenoamide Compounds
Authors: Lazo-Jiménez Rosa E. , Ortega-Alfaro María C. , López- Cortés José G. , Chávez-Carvayar José A. , Ignés-Mullol Jordi , Sagués Francesc , Álvarez-Venicio Violeta , Carreón-Castro María P. 
Published: February 2014

ABSTRACTIn this work, the synthesis of two amphiphilic π-conjugated compounds such as ferrocenylthioamide and ferrocenylselenoamide, with the general formula FcC=MNH(CH2)15CH3 with M = S or Se, are reported. The ferrocenyl group is a donor moiety forming a π-conjugated system with the amides of sulfur and selenium; both elements have also bioactivity with pharmacological interest. These two compounds formed Langmuir (L) monolayers at the air-water interface, which were characterized by isotherms of surface pressure versus molecular area (π-A) and compression/expansion cycles (hysteresis curves); Brewster angle microscopic images were also obtained. By using the Langmuir-Blodgett method molecular monolayers were transferred onto glass substrates. These nanostructures, in form of Langmuir-Blodgett (LB) films, were characterized through atomic force microscopy (AFM).
Association Between Obesity and Periodontitis in Pregnant Females
Authors: Lee Hyo‐Jin , Jun Jong‐Kwan , Lee Seung‐Mi , Ha Jung‐Eun , Paik Dai‐Il , Bae Kwang‐Hak 
Published: February 2014

Background: The purpose of this study is to investigate whether overweight and obesity before pregnancy are associated with periodontitis during pregnancy.Methods: This study examined a total of 315 pregnant females at 21 to 24 weeks of gestation. Overweight and obesity were defined based on criteria proposed by the World Health Organization Expert Consultation. Periodontal conditions were assessed by measuring clinical periodontal attachment loss (AL). To investigate whether obese pregnant females have increased risk according to the extent of periodontitis, the data were divided into two groups: 1) generalized periodontitis and 2) localized periodontitis. A comparison among underweight, normal‐weight, and overweight/obese groups for explanatory variables was analyzed using the χ2 test for categorical variables and an analysis of variance for continuous variables. Multivariate logistic regression analysis was performed with adjustments for age, health and oral health behaviors, and obstetric information.Results: Age, age at first delivery, periodontitis, and periodontal conditions (two or more interproximal sites with AL ≥4 mm not on the same tooth) were significantly associated with body mass index (BMI) (P <0.05). The adjusted odds ratio of periodontitis was 4.57 (95% confidence interval = 2.30 to 9.07) for overweight and obese females (BMI ≥23 kg/m2), after adjusting for all of the covariates.Conclusion: There is a strong association between prepregnancy overweight/obesity and periodontitis in pregnant females.
Effects of Locally Administered Tiludronic Acid on Experimental Periodontitis in Rats
Authors: Furlaneto Flávia A.C. , Nunes Nara L.T. , Oliveira Filho Ivan L. , Frota Nicolly P.R. , Yamamoto Kely O. , Lisboa Mario R.P. , Ervolino Edilson , Taba Mario , Rêgo Rodrigo Otávio , Messora Michel R. 
Published: February 2014

Background: It appears there are no studies evaluating the influence of the bisphosphonate tiludronic acid (TIL) on periodontitis. The purpose of this study is to evaluate via microtomographic, histopathologic, histometric, and immunohistochemical analyses the effects of local administration of TIL on ligature‐induced periodontitis in rats.Methods: Forty‐eight rats were divided into six groups: C (control), EP (experimental periodontitis), EP‐Saline, EP‐TIL0.1, EP‐TIL0.3, and EP‐TIL1. In EP, a ligature was placed around maxillary second molars. In EP‐TIL0.1, EP‐TIL0.3, and EP‐TIL1, TIL solutions of 0.1, 0.3, and 1 mg/kg body weight, respectively, were injected into the subperiosteal palatal area adjacent to maxillary second molars every other day. EP‐Saline received 0.9% NaCl solution instead. Animals were euthanized at day 11. Bone changes were evaluated by microtomographic and histometric analyses. Histopathologic analysis and immunohistochemical detection of tartrate‐resistant acid phosphatase (TRAP) were also performed. Data were statistically analyzed (analysis of variance or Kruskal–Wallis, P <0.05).Results: Histometric and microtomographic analyses (at buccal, interproximal, and furcation sites) demonstrated that EP‐TIL1 presented less alveolar bone loss (ABL) than EP (P <0.05), whereas EP‐TIL0.1 and EP‐TIL0.3 did not demonstrate significant differences in alveolar bone level compared to EP (P >0.05). Also, EP‐TIL1 showed significantly fewer TRAP‐positive multinucleated osteoclasts than EP and EP‐Saline (P <0.05).Conclusion: It can be concluded that locally administered TIL solution (1 mg/kg body weight) reduced alveolar bone loss in experimental periodontitis and the dosage of TIL may influence its anti‐inflammatory and antiresorptive properties.
Salivary Biomarkers Associated With Gingivitis and Response to Therapy
Authors: Syndergaard Ben , Al‐Sabbagh Mohanad , Kryscio Richard J. , Xi Jing , Ding Xiuhua , Ebersole Jeffrey L. , Miller Craig S. 
Published: February 2014

Background: Salivary biomarkers are potentially important for determining the presence, risk, and progression of periodontal disease. However, clinical translation of biomarker technology from lab to chairside requires studies that identify biomarkers associated with the transitional phase between health and periodontal disease (i.e., gingivitis).Methods: Eighty participants (40 with gingivitis, 40 healthy) provided saliva at baseline and 7 to 30 days later. An additional sample was collected from gingivitis participants 10 to 30 days after dental prophylaxis. Clinical parameters of gingival disease were recorded at baseline and the final visit. Salivary concentrations of interleukin (IL)‐1β, IL‐6, matrix metalloproteinase (MMP)‐8, macrophage inflammatory protein (MIP)‐1α, and prostaglandin E2 (PGE2) were measured.Results: Clinical features of health and gingivitis were stable at both baseline visits. Participants with gingivitis demonstrated significantly higher bleeding on probing (BOP), plaque index (PI), and gingival index (GI) (P ≤0.002) and a significant drop in BOP, PI, and GI post‐treatment (P ≤0.001). Concentrations of MIP‐1α and PGE2 were significantly higher (2.8 times) in the gingivitis group than the healthy group (P ≤0.02). After dental prophylaxis, mean biomarker concentrations did not decrease significantly from baseline in the gingivitis group, although concentrations of IL‐1β, IL‐6, and MMP‐8 approached healthy levels, whereas MIP‐1α and PGE2 concentrations remained significantly higher than in the healthy group (P ≤0.04). Odds ratio analyses showed that PGE2 concentrations, alone and in combination with MIP‐1α, readily discriminated gingivitis from health.Conclusions: Salivary PGE2 and MIP‐1α discriminate gingivitis from health, and patients with gingivitis who return to clinical health continue to produce inflammatory mediators for weeks after dental prophylaxis.
Determinants of Intrauterine Contraceptive Device Use Among the Women of Urban Areas of Nepal
Authors: Joshi R , Bhattarai S , Simkhada K , Thapa S 
Published: February 2014

Aims: This study aimed to identify the determinants of Intrauterine Contraceptive Device (IUCD) use among the married women of reproductive age group living in urban areas of Nepal. Methods: This study was an analytical cross-sectional study which aimed to compare different factors between 110 IUCD users and 110 non-users. Both groups were selected randomlyand interviewed by using semi-structured questionnaire. Chi-square test was used to detect the difference between two groups and corresponding odds ratios with 95% confidence intervals were also computed. Results: When the IUCD users were compared to non-users, religion, occupational status of women, occupational status of the husbands, total number of children, sources of information about IUCD, having wrong beliefs about IUCD, availability of IUCD services, reproductive intention and spousal communication were significantly associated with the use of IUCD.The main reason for using IUCD was due to the effectiveness for longer duration and for not using IUCD was due to the feeling of no need. Conclusions: Wrong beliefs about the device discouraged women from using IUCD, hence, awareness programs are strongly recommended.Nepal Journal of Obstetrics and Gynaecology / Vol 8 / No. 2 / Issue 16 / July-Dec, 2013 / 16-20 DOI: http://dx.doi.org/10.3126/njog.v8i2.9760