bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of gene expression of intracellular calcium channels, pumps and exchangers with epidermal growth factor-induced epithelial-mesenchymal transition in a breast cancer cell line
Authors: Davis Felicity M , Parsonage Michelle T , Cabot Peter J , Parat Marie-Odile , Thompson Erik W , Roberts-Thomson Sarah J , Monteith Gregory R 
Published: July 2013

Abstract

Background
Epithelial-mesenchymal transition (EMT) is a process implicated in cancer metastasis that involves the conversion of epithelial cells to a more mesenchymal and invasive cell phenotype. In breast cancer cells EMT is associated with altered store-operated calcium influx and changes in calcium signalling mediated by activation of cell surface purinergic receptors. In this study, we investigated whether MDA-MB-468 breast cancer cells induced to undergo EMT exhibit changes in mRNA levels of calcium channels, pumps and exchangers located on intracellular calcium storing organelles, including the Golgi, mitochondria and endoplasmic reticulum (ER).

Methods
Epidermal growth factor (EGF) was used to induce EMT in MDA-MB-468 breast cancer cells. Serum-deprived cells were treated with EGF (50 ng/mL) for 12 h and gene expression was assessed using quantitative RT-PCR.

Results and conclusions
These data reveal no significant alterations in mRNA levels of the Golgi calcium pump secretory pathway calcium ATPases (SPCA1 and SPCA2), or the mitochondrial calcium uniporter (MCU) or Na+/Ca2+ exchanger (NCLX). However, EGF-induced EMT was associated with significant alterations in mRNA levels of specific ER calcium channels and pumps, including (sarco)-endoplasmic reticulum calcium ATPases (SERCAs), and inositol 1,4,5-trisphosphate receptor (IP3R) and ryanodine receptor (RYR) calcium channel isoforms. The most prominent change in gene expression between the epithelial and mesenchymal-like states was RYR2, which was enriched 45-fold in EGF-treated MDA-MB-468 cells. These findings indicate that EGF-induced EMT in breast cancer cells may be associated with major alterations in ER calcium homeostasis.
The anti-inflammatory effect of kaempferol on early atherosclerosis in high cholesterol fed rabbits
Authors: Kong Lingxi , Luo Cheng , Li Xiuying , Zhou Yuanda , He Haixia 
Published: July 2013

AbstractBackgroundAtherosclerosis has been widely accepted as an inflammatory disease of vascular, adhesion molecules play an important role in the early progression of it. The aim of the present study was to evaluate the effect of kaempferol on the inflammatory molecules such as E-selectin (E-sel), intercellular adhesion molecule-1 (ICAM-1), vascular cell adhesionmolecule-1 (VCAM-1) and monocyte chemotactic protein-1 (MCP-1) in high cholesterol induced atherosclerosis rabbit models.MethodsThirty male New Zealand white (NZW) rabbits were randomly divided into five groups, control group, model group, fenofibrate (12mg/kg) group and kaempferol groups (150 mg/kg and 30 mg/kg). The rabbits were fed with a normal diet or a high cholesterol diet for 10 weeks. Levels of blood lipids, serum tumour-necrosis factor-alpha (TNF-α) and serum interleukin-1beta (IL-1β) were detected at the end of the sixth and tenth week. Malonaldehyde (MDA) level and superoxide dismutase (SOD) activity in serum were also determined. Lesion areas of the aorta were measured with morphometry analysis after ten weeks. Gene expression of E-sel, ICAM-1, VCAM-1 and MCP-1 in aortas was determined by RT-PCR (reverse transcription-polymerase chain reaction). Immunohistochemical staining was employed to measure protein expression of E-sel, ICAM-1, VCAM-1 and MCP-1.ResultsModel rabbits fed with ten weeks of high-cholesterol diet developed significant progression of atherosclerosis. Compared with the control, levels of blood lipids, TNF-α, IL-1β and MDA increased markedly in serum of model rabbits, while SOD levels decreased. Gene and protein expressions of E-sel, ICAM-1, VCAM-1 and MCP-1 in atherosclerotic aortas increased remarkably in model group. However, comparing to the model rabbits, levels of TNF-α, IL-1β and MDA decreased significantly and serum SOD activity increased, gene and protein expressions of E-sel, ICAM-1, VCAM-1 and MCP-1 in aortas decreased significantly with the treatment of kaempferol.ConclusionKaempferol shows anti-atherosclerotic effect by modulating the gene and protein expression of inflammatory molecules.
Mitochondrial translocation of cofilin is required for allyl isothiocyanate-mediated cell death via ROCK1/PTEN/PI3K signaling pathway
Authors: Li Guo-bing , Cheng Qi , Liu Lei , Zhou Ting , Shan Chang-yu , Hu Xiao-ye , Zhou Jing , Liu E-hu , Li Ping , Gao Ning 
Published: July 2013

Abstract

Background
Cofilin is a member of the actin depolymerizing factor (ADF)/cofilin family, which regulates actin dynamics. Increasing evidence suggests that mitochondrial translocation of cofilin appears necessary for the regulation of apoptosis.

Results
We report that allyl isothiocyanate (AITC) potently induces mitochondria injury and apoptosis. These events were accompanied by a loss of polymerized filamentous actin (F-actin) and increase in unpolymerized globular actin (G-actin). AITC also induces dephosphorylation of cofilin through activation of PP1 and PP2A. Only dephosphorylated cofilin binds to G-actin and translocates to mitochondria during AITC-mediated apoptosis. Mechanistic study revealed that interruption of ROCK1/PTEN/PI3K signaling pathway plays a critical role in AITC-mediated dephosphorylation and mitochondrial translocation of cofilin and apoptosis. Our in vivo study also showed that AITC-mediated inhibition of tumor growth of mouse leukemia xenograft model is in association with dephosphorylation of cofilin.

Conclusions
These findings support a model in which induction of apoptosis by AITC stems primarily from activation of ROCK1 and PTEN, and inactivation of PI3K, leading in turn to activation of PP1 and PP2A, resulting in dephosphorylation of cofilin, which binds to G-actin and translocates to mitochondria, culminating in the dysfunction of mitochondria, release of cytochrome c and apoptosis.
The PAMPA datasets: a metagenomic survey of microbial communities in Argentinean pampean soils
Authors: Rascovan Nicolás , Carbonetto Belén , Revale Santiago , Reinert Marina D , Alvarez Roberto , Godeas Alicia M , Colombo Roxana , Aguilar Mario , Novas María Victoria , Iannone Leopoldo , Zelada Alicia M , Pardo Alejandro , Schrauf Gustavo , Mentaberry Alejandro , Vazquez Martín P 
Published: July 2013

Abstract

Background
Soil is among the most diverse and complex environments in the world. Soil microorganisms play an essential role in biogeochemical cycles and affect plant growth and crop production. However, our knowledge of the relationship between species-assemblies and soil ecosystem processes is still very limited. The aim of this study was to generate a comprehensive metagenomic survey to evaluate the effect of high-input agricultural practices on soil microbial communities.

Results
We collected soil samples from three different areas in the Argentinean Pampean region under three different types of land uses and two soil sources (bulk and rhizospheric). We extracted total DNA from all samples and also synthetized cDNA from rhizospheric samples. Using 454-FLX technology, we generated 112 16S ribosomal DNA and 14 16S ribosomal RNA amplicon libraries totaling 1.3 M reads and 36 shotgun metagenome libraries totaling 17.8 million reads (7.7 GB). Our preliminary results suggested that water availability could be the primary driver that defined microbial assemblages over land use and soil source. However, when water was not a limiting resource (annual precipitation >800 mm) land use was a primary driver.

Conclusion
This was the first metagenomic study of soil conducted in Argentina and our datasets are among the few large soil datasets publicly available. The detailed analysis of these data will provide a step forward in our understanding of how soil microbiomes respond to high-input agricultural systems, and they will serve as a useful comparison with other soil metagenomic studies worldwide.
Epilepsy: neuroinflammation, neurodegeneration, and APOE genotype
Authors: Aboud Orwa , Mrak Robert E , Boop Frederick A , Griffin W Sue T 
Published: July 2013

Abstract

Background
Precocious development of Alzheimer-type neuropathological changes in epilepsy patients, especially in APOE ϵ4,4 carriers is well known, but not the ways in which other APOE allelic combinations influence this outcome. Frozen and paraffin-embedded tissue samples resected from superior temporal lobes of 92 patients undergoing temporal lobectomies as a treatment for medication-resistant temporal lobe epilepsy were used in this study. To determine if epilepsy-related changes reflect those in another neurological condition, analogous tissue samples harvested from 10 autopsy-verified Alzheimer brains, and from 10 neurologically and neuropathologically normal control patients were analyzed using immunofluorescence histochemistry, western immunoblot, and real-time PCR to determine genotype effects on neuronal number and size, neuronal and glial expressions of amyloid β (Aβ) precursor protein (βAPP), Aβ, apolipoprotein E (ApoE), S100B, interleukin-1α and β, and α and β secretases; and on markers of neuronal stress, including DNA/RNA damage and caspase 3 expression.

Results
Allelic combinations of APOE influenced each epilepsy-related neuronal and glial response measured as well as neuropathological change. APOE ϵ3,3 conferred greatest neuronal resilience denoted as greatest production of the acute phase proteins and low neuronal stress as assessed by DNA/RNA damage and caspase-3 expression. Among patients having an APOE ϵ2 allele, none had Aβ plaques; their neuronal sizes, like those with APOE ϵ3,3 genotype were larger than those with other genotypes. APOE ϵ4,4 conferred the weakest neuronal resilience in epilepsy as well as in Alzheimer patients, but there were no APOE genotype-dependent differences in these parameters in neurologically normal patients.

Conclusions
Our findings provide evidence that the strength of the neuronal stress response is more related to patient APOE genotype than to either the etiology of the stress or to the age of the patient, suggesting that APOE genotyping may be a useful tool in treatment decisions.
Conversion of C6 and C5 sugars in undetoxified wet exploded bagasse hydrolysates using Scheffersomyces (Pichia) stipitis CBS6054
Authors: Biswas Rajib , Uellendahl Hinrich , Ahring Birgitte K 
Published: July 2013

Abstract
Sugarcane bagasse is a potential feedstock for cellulosic ethanol production, rich in both glucan and xylan. This stresses the importance of utilizing both C6 and C5 sugars for conversion into ethanol in order to improve the process economics. During processing of the hydrolysate degradation products such as acetate, 5-hydroxymethylfurfural (HMF) and furfural are formed, which are known to inhibit microbial growth at higher concentrations. In the current study, conversion of both glucose and xylose sugars into ethanol in wet exploded bagasse hydrolysates was investigated without detoxification using Scheffersomyces (Pichia) stipitis CBS6054, a native xylose utilizing yeast strain. The sugar utilization ratio and ethanol yield (Yp/s) ranged from 88-100% and 0.33-0.41 ± 0.02 g/g, respectively, in all the hydrolysates tested. Hydrolysate after wet explosion at 185°C and 6 bar O2, composed of mixed sugars (glucose and xylose) and inhibitors such as acetate, HMF and furfural at concentrations of 3.2 ± 0.1, 0.4 and 0.5 g/l, respectively, exhibited highest cell growth rate of 0.079 g/l/h and an ethanol yield of 0.39 ± 0.02 g/g sugar converted. Scheffersomyces stipitis exhibited prolonged fermentation time on bagasse hydrolysate after wet explosion at 200°C and 6 bar O2 where the inhibitors concentration was further increased. Nonetheless, ethanol was produced up to 18.7 ± 1.1 g/l resulting in a yield of 0.38 ± 0.02 g/g after 82 h of fermentation.
Dziwna etniczność
Authors: Wasyl Franciszek 
Published: July 2013

Францішек Василь, Дивна етнічність. Форми вірменської ідентичності в Галичині
Стаття присвячена питанню про ідентичність польських вірменів в Галичині. Центральною постаттю аналізу у тексті є польсько- та русинськомовний австрійський барон та палкий польський патріот і господар вірменський в одній особі – Григорій Ромашкан. Метою цієї статті є на згаданому прикладі довести існування окрім традиційних етнічних маркерів як мова та віросповідання інших важливих для польських вірменів чинників збереження ідентичності. Автору вдалося довести, що складовими етнічної ідентичності цієї групи є пам’ять про минуле родини, обрядовість та переконання про етнічну самобутність. Автор переконаний у тому, що через їх емоційний характер вони западали у пам’ять частіше ніж інші швидкоплинні життєві події. Тим більше, що участь в них гуртувала представників багатьох соціальних верств, поколінь тобто переважної більшості невеликого числа місцевої вірменської громади. Таким чином урочистості на цвинтарі чи у костелі ставали простором у якому гартувалась вірменська ідентичність. Разом із тим автор дійшов висновку, що тип ідентичності польських вірменів в Галичині можна окреслити як сентиментальний. Адже пам’ять про родинні місця та емоційно важливі події впливає на особистість сильніше ніж сповідуванні ідеології та акультурація. У статті використано мемуаристику та метричні книги і списки віруючих.
Caritas na pograniczu kulturowym
Authors: Białoń Adam 
Published: July 2013

Адам Бялонь, «Карітас» на культурному пограниччі. Римо-католицькі моделі та їх вплив на форми благодійності Греко-католицької Церкви в Галичині
Розвиток форм організованої доброчинності в Римсько-католицькій як і в Грекокатолицькій церквах з самого початку виявляв виразні диспропорції у своєму масштабі і динаміці. Масова полонізація руських еліт причинялася до того, що спочатку Православна, а пізніше Греко-католицька Церква безповоротно втрачали впливових опікунів і меценатів однаково церковних структур як і окремих сфер діяльності Церкви, в тому, й добродійної діяльності. Скромні умови матеріального буття всієї установи Греко-католицької Церкви, порівняно з Римсько-католицькою, а також слабка активність її духівництва в суспільній сфері, засуджували її доброчинність на вегетацію. Структурноорганізаційне оживлення Греко-католицької Церкви на території Галичині в другій половині XIX віку і опора розвитку його структур на зразках, запозичених з Римсько-католицької Церкви, спричинило динамічний розвиток різних форм організованої добродійної діяльності. У міжвоєнний період почали з’являтися нові діючі монаші згромадження на зразок на римсько-католицьких а також сучасні добродійні організації у всіх грекoкатолицьких єпархіях.
Providing Critical Laboratory Results on Time, Every Time to Help Reduce Emergency Department Length of Stay
Authors: Blick Kenneth E. 
Published: July 2013

Abstract

Objectives:
To develop a fully automated core laboratory, handling samples on a “first in, first out” real-time basis with Lean/Six Sigma management tools.

Methods:
Our primary goal was to provide services to critical care areas, eliminating turnaround time outlier percentage (TAT-OP) as a factor in patient length of stay (LOS). A secondary goal was to achieve a better laboratory return on investment.

Results:
In 2011, we reached our primary goal when we calculated the TAT-OP distribution and found we had achieved a Six Sigma level of performance, ensuring that our laboratory service can be essentially eliminated as a factor in emergency department patient LOS. We also measured return on investment, showing a productivity improvement of 35%, keeping pace with our increased testing volume.

Conclusions:
As a result of our Lean process improvements and Six Sigma initiatives, in part through (1) strategic deployment of point-of-care testing and (2) core laboratory total automation with robotics, middleware, and expert system technology, physicians and nurses at the Oklahoma University Medical Center can more effectively deliver lifesaving health care using evidence-based protocols that depend heavily on “on time, every time” laboratory services.
68Ge/68Ga production revisited: excitation curves, target preparation and chemical separation – purification
Authors: Adam-Rebeles R. , Hermanne A. , Van den Winkel P. , De Vis L. , Waegeneer R. , Tárkányi F. , Takács S. , Takács M. P. 
Published: July 2013

SummaryTargets withstanding high beam power for production of68Ge by proton irradiation of Ga were developed using electrodeposition of Ga/Ni alloy (70/30%) on thick Cu backings. Cross sections for reactions, induced by protons (up to 36MeV) and deuterons (up to 50MeV), producing γ-emitting radioisotopes with half-lives longer than 2 h were measured in a stacked-foil irradiation using thin Ga/Ni targets produced with this technique. Excitation functions for68,69Ge are discussed, relative to the monitor reactionsnatCu(p, x)62Zn ornatAl(d, x)24,22Na. The results are compared to the scarce literature values. From derived thick target yields, optimal energy domain and batch yields for68Ge production were calculated and contamination (from69Ge and71Ge) is discussed. A PC-controlled, automated dissolution and separation chemistry set-up for delivery of nca69Ge was developed (yield about 90%) and implemented in an industrial system ready to be mounted in a hot cell.
Globalizing Mediterranean Identities
Authors: Boozer Anna Lucille 
Published: July 2013

This article furthers recent gains made in applying globalization perspectives to the Roman world by exploring
two Romano-Egyptian houses that used Roman material culture in different ways within the city known
as Trimithis (modern day Amheida, in Egypt). In so doing, I suggest that concepts drawn from globalization
theory will help us to disentangle and interpret how homogeneous Roman Mediterranean goods may appear
heterogeneous on the local level. This theoretical vantage is broadly applicable to other regions in the Roman
Mediterranean, as well as other environments in which individuals reflected a multifaceted relationship
with their local identity and the broader social milieu.
Granulation of After Reclamation Dusts from the Mixed Sands Technology: Water Glass – Resolit / Granulacja pyłów poregeneracyjnych z technologii mas mieszanych: szkło wodne – rezolit
Authors: Kamińska J. , Dańko J. 
Published: July 2013

Abstract
A technology of sands with water glass hardened by liquid esters is a cheap and ecologic method of producing moulding sands. Due to these advantages, this technology is still very important in several foundry plants for production of heavy iron and steel castings. Reclamation of the mixed moulding and core sands generates significant amounts of dusts, which require further treatments for their reuse. The results of investigations of a pressureless granulation of dusts generated in the dry mechanical reclamation process of the mixture consisting in app. 90 % of moulding sands from the Floster S technology and in 10 % of core sands with phenolic resin resol type, are presented in the hereby paper. Investigations were aimed at obtaining granulates of the determined dimensional and strength parameters.
Granules were formed from the mixture of dusts consisting of 75 mass% of dusts after the reclamation of sands mixture and of 25 mass% of dusts from bentonite sands processing plant. Wetted dusts from bentonite sands were used as a binding agent allowing the granulation of after reclamation dusts originated from the mixed sands technology.
Study of the BA1044 Alloy Properties and Their Interpretation with the Use of Fuzzy Logic
Authors: Górny Z. , Kluska -Nawarecka S. , Wilk-Kołodziejczyk D. , Saja K. 
Published: July 2013

Abstract
The study presents the results of research aimed at the construction of a model of the relationship between the physical properties of metal and the types of toughening treatment and modifiers used in the modification of BA1044 alloy.
Samples of melts were subjected to four variants of the heat treatment and to five types of modification. Studies of the samples consisted in measurements of five physical parameters. Consequently, it was necessary to seek a relationship between the nine input parameters and five output parameters. With this number of the variables and a limited number of samples, searching for the relationships by way of statistical methods was obviously impossible, so it was decided to create an approximate model through the use of fuzzy logic. This study describes the process of creating a model and presents the results of some simulation experiments that confirm the validity of the correct approach.
Investigations of the Temperature Influence on Formation of Compounds from the BTEX Group During the Thermal Decomposition of Furan Resin / Badania wpływu temperatury na powstawanie związków z grupy BTEX podczas termicznego rozkładu żywicy furanowej
Authors: Kubecki M. , Holtzer M. , Żymankowska-Kumon S. 
Published: July 2013

Abstract
Organic binders applied in foundry plants based on synthetic resins, from the one side influence obtaining the required technological properties by the moulding sand and - in consequence - obtaining good quality castings, and on the other side are the source of volatile organic compounds (VOC). Together with synthetic resins their hardeners, which although added in very small amounts emit during their thermal decomposition substances negatively influencing the natural environment, are also used. Both, resins and hardeners only at the influence of high temperatures accompanying moulds pouring with liquid metal generate harmful volatile organic compounds including compounds from the BTEX group. Investigations of the temperature influence on the kind and amount of organic compounds formed during the thermal decomposition of selected binders and hardeners and their mixtures allow to determine temperature ranges the most favourable for emitting harmful substances as well as to compare their emission from the selected materials.
The aim of this study was the determination the temperature influence on formation substances from the BTEX group, during thermal decomposition of the selected binder, its hardener and their mixture. The BTEX group emission constitutes one of the basic criteria in assessing the harmfulness of materials applied for moulding and core sands and it can undergo changes in dependence of the applied system resin-hardener. Investigations were carried out on the specially developed system for the thermal decomposition of organic substances in the temperature range: 500ºC - 1300ºC, at the laboratory scale. The investigations subject was the furan resin, its hardener and hardened furan resin. The assessment of the emission degree of the BTEX group in dependence of the system subjected to the temperature influence was performed, within the studies. The temperature range, in which maximal amounts of benzene, toluene, ethylbenzene and xylenes were emitted from tested materials - was defined. The qualitative and quantitative analysis of the BTEX group were carried out with using the gas chromatography technique coupled with the mass spectrometry (GC/MS).