bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined 585 nm pulsed‐dye and 1,064 nm Nd:YAG lasers for the treatment of basal cell carcinoma
Authors: Jalian H. Ray , Avram Mathew M. , Stankiewicz Kelly J. , Shofner Joshua D. , Tannous Zeina 
Published: November 2013

Background and ObjectiveBasal cell carcinomas (BCCs) have supporting vasculature that serves as a target for vascular selective lasers. The objective of this study was to determine the effect of repeated treatment with a combined 585 nm pulsed dye laser (PDL) and 1,064 nm Neodymium Yttrium Aluminum Garnet (Nd:YAG) laser on BCCs of superficial and nodular subtypes of varying diameters.Study Design/Materials and MethodsTen subjects with 13 biopsy‐proven BCCs received four combined PDL and Nd:YAG at treatments 2–4 week intervals. None of the BCCs met the criteria for Mohs micrographic surgery. The tumor and 4 mm of peripheral skin were treated using standardized parameters delivered with a 7 mm spot with 10% overlap. The treated area was excised and evaluated histologically for residual tumor. The primary study endpoint was histologic clearance of tumor. The secondary study endpoint was blinded investigator assessment of clinical endpoint and adverse effects.ResultsApproximately half of all tumors showed a complete response to four combined PDL and Nd:YAG treatments (n = 7/12, 58%). When stratified by size, 75% of all tumors <1 cm in diameter (n = 6/8) showed complete response. Tumor histologic types among the complete responders included superficial and nodular BCCs. All subjects with incompletely responding BCCs were on various forms of anticoagulation, which we hypothesize, may inhibit laser‐mediated thrombosis necessary for the clinical effect. Blinded investigator assessment suggests that biopsy related erythema improves with subsequent laser treatments.ConclusionsCombined PDL and Nd:YAG laser is an effective means of reducing tumor burden in patients with BCC and may be a promising, emerging alternative therapy. Factors influencing treatment response includes the concomitant use of anticoagulation. Further studies are needed to investigate and optimize the utility of this treatment protocol. Lasers Surg. Med. 46:1–7, 2014. © 2013 Wiley Periodicals, Inc.
Intraoperative use of spectroscopic detection of protoporphyrin IX from oral 5‐aminolevulinic acid on basal cell carcinoma and squamous cell carcinoma during Mohs surgery
Authors: Aoyagi Satoru , Hata Hiroo , Homma Erina , Horie Keita , Shimizu Hiroshi 
Published: November 2013

Background and ObjectivePhotodynamic diagnosis (PDD) using 5‐aminolevulinic acid (5‐ALA) has been used as an in vivo presurgical diagnostic tool for detecting occult tumor borders of ill‐defined basal cell carcinomas (BCCs), especially before Mohs micrographic surgery (MMS). However, most techniques reported in the literature involve gross examination or various digital detection systems to observe the amount of red light fluorescence after topical application of 5‐ALA. This study aimed to conduct a preliminary examination on the accuracy of intraoperative use of PDD by spectroscopic detection from oral 5‐ALA to identify tumor fluorescence from the peritumoral surgical pieces of tissue.Materials and MethodsQualitative evaluation of fluorescence spectroscopic detection was performed on 77 specimens from 13 patients with high‐risk BCC and 10 patients with high‐risk squamous cell carcinoma (SCC). All patients were orally administered with 5‐ALA preoperatively. The excised specimens were intraoperatively evaluated by scanning of the fluorescence spectrum. Results were compared with those of histopathology.ResultsOverall sensitivity and specificity were, respectively, 100% and 84.3% for spectroscopic detection. Although the negative predictive value was 100%, the positive predictive value was not sufficiently high for BCC (20%) nor for SCC (62.5%).ConclusionFluorescence spectroscopic detection is as a step towards a possibly useful technique for decreasing the number of excisions in MMS. In its current state, however, it has no substantial clinical advantage. Lasers Surg. Med. 46:132–139, 2014. © 2013 Wiley Periodicals, Inc.
Inductively coupled plasma mass spectrometry‐based immunoassay: A review
Authors: Liu Rui , Wu Peng , Yang Lu , Hou Xiandeng , Lv Yi 
Published: November 2013

AbstractThe last 10 years witnessed the emerging and growing up of inductively coupled plasma mass spectrometry (ICPMS)‐based immunoassay. Its high sensitivity and multiplex potential have made ICPMS a revolutionary technique for bioanalyte quantification after element‐tagged immunoassay. This review focuses on the major developments and the applications of ICPMS‐based immunoassay, with emphasis on methodological innovations. The ICPMS‐based immunoassay with elemental tags of metal ions, nanoparticles, and metal containing polymers was discussed in detail. The recent development of multiplex assay, mass cytometry, suspension array, and surface analysis demonstrated the versatility and great potential of this technique. ICPMS‐based immunoassay has become one of the key methods in bioanalysis. © 2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 373–393, 2014
Jean‐Antoine Nollet: The father of experimental electrospray
Authors: Dumont Quentin , Cole Richard B. 
Published: November 2013

AbstractThe development of electrospray ionization mass spectrometry (ESI‐MS) was a 20th century occurrence that underwent rapid acceleration especially in the 1990's. However, long prior to its coupling with mass spectrometry, the electrification of liquids had been studied in a variety of contexts. Although initial reports describing cone formation upon electrification of water drops came out of England, the first true experiments investigating the electrospray phenomenon were performed in the middle of the 18th century by Abbé Jean‐Antoine Nollet. The current report, associated with the French Regional Issue of Mass Spectrometry Reviews, examines the contributions of Abbé Nollet to the earliest understanding of the electrospray phenomenon. A description of his accomplishments is placed in the context of the societal and scientific developments of the “Age of Enlightenment” out of which Jean‐Antoine Nollet arose. © 2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 418–423, 2014.
GABAA receptor modulation by terpenoids from Sideritis extracts
Authors: Kessler Artur , Sahin‐Nadeem Hilal , Lummis Sarah C. R. , Weigel Ingrid , Pischetsrieder Monika , Buettner Andrea , Villmann Carmen 
Published: November 2013

ScopeGABAA receptors are modulated by Sideritis extracts. The aim of this study was to identify single substances from Sideritis extracts responsible for GABAA receptor modulation.Methods and resultsSingle volatile substances identified by GC have been tested in two expression systems, Xenopus oocytes and human embryonic kidney cells. Some of these substances, especially carvacrol, were highly potent on GABAA receptors composed of α1β2 and α1β2γ2 subunits. All effects measured were independent from the presence of the γ2 subunit. As Sideritis extracts contain a high amount of terpenes, 13 terpenes with similar structure elements were tested in the same way. Following a prescreening on α1β2 GABAA receptors, a high‐throughput method was used for identification of the most effective terpenoid substances on GABA‐affinity of α1β2γ2 receptors expressed in transfected cell lines. Isopulegol, pinocarveol, verbenol, and myrtenol were the most potent modifiers of GABAA receptor function.ConclusionComparing the chemical structures, the action of terpenes on GABAA receptors is most probably due to the presence of hydroxyl groups and a bicyclic character of the substances tested. We propose an allosteric modulation independent from the γ2 subunit and similar to the action of alcohols and anesthetics.
Short repetition time multiband echo‐planar imaging with simultaneous pulse recording allows dynamic imaging of the cardiac pulsation signal
Authors: Tong Yunjie , Hocke Lia M. , Frederick Blaise deB. 
Published: November 2013

PurposeRecently developed simultaneous multislice echo‐planar imaging (EPI) sequences permit imaging of the whole brain at short repetition time (TR), allowing the cardiac fluctuations to be fully sampled in blood‐oxygen‐level dependent functional MRI (BOLD fMRI). A novel low computational analytical method was developed to dynamically map the passage of the pulsation signal through the brain and visualize the whole cerebral vasculature affected by the pulse signal. This algorithm is based on a simple combination of fast BOLD fMRI and the scanner's own built‐in pulse oximeter.MethodsMultiple, temporally shifted copies of the pulse oximeter data (with 0.08 s shifting step and coverage of a 1‐s span) were downsampled and used as cardiac pulsation regressors in a general linear model based analyses (FSL) of the fMRI data. The resulting concatenated z‐statistics maps show the voxels that are affected as the cardiac signal travels through the brain.ResultsMany voxels were highly correlated with the pulsation regressor or its temporally shifted version. The dynamic and static cardiac pulsation maps obtained from both the task and resting state scans, resembled cerebral vasculature.ConclusionThe results demonstrated: (i) cardiac pulsation significantly affects most voxels in the brain; (ii) combining fast fMRI and this analytical method can reveal additional clinical information to functional studies. Magn Reson Med 72:1268–1276, 2014. © 2013 Wiley Periodicals, Inc.
B1 estimation using adiabatic refocusing: BEAR
Authors: Jordanova Kalina V. , Nishimura Dwight G. , Kerr Adam B. 
Published: November 2013

PurposeAccurate measurement of the nonuniform transmit radiofrequency field is useful for many applications in magnetic resonance imaging, such as calibrating the scanner transmit system, evaluating coil performance, and improving image quality and quantitation. The radiofrequency field excitation amplitude (B1) is often obtained by acquiring a B1 map. In this study, a new B1 mapping method is proposed.Theory and MethodsThe use of two adiabatic full passage pulses with different magnitudes applied as successive refocusing pulses results in a linear relationship between phase and B1 field strength that is insensitive to the repetition time, off‐resonance effects, T1, and T2. Using this method, B1 mapping can be localized to a slice or three‐dimensional (3D) volume, with a spin‐echo acquisition that is appropriate for fast projection measurements.ResultsThis new method is shown to agree well with the Bloch‐Siegert B1 mapping method for both phantom and in vivo B1 measurements at 1.5T, 3T, and 7T. The method's ability to acquire accurate projection B1 measurements is also demonstrated.ConclusionThis method's high dynamic range, ability to make fast projection measurements, and linear quantitative relationship between phase and B1 make it an ideal candidate for use in robust transmitter gain calibration. Magn Reson Med 72:1302–1310, 2014. © 2013 Wiley Periodicals, Inc.
Electrical impedance myography correlates with standard measures of Als severity
Authors: Rutkove Seward B. , Caress James B. , Cartwright Michael S. , Burns Ted M. , Warder Judy , David William S. , Goyal Namita , Maragakis Nicholas J. , Benatar Michael , Sharma Khema R. , Narayanaswami Pushpa , Raynor Elizabeth M. , Watson Mary Lou , Shefner Jeremy M. 
Published: November 2013

ABSTRACTIntroduction: Electrical impedance myography (EIM) can be used to assess amyotrophic lateral sclerosis (ALS) progression. The relationship between EIM values and standard assessment measures, however, is unknown. Methods: EIM 50 kHz phase data from 60 subjects who participated in a longitudinal natural history study of ALS were correlated with handheld dynamometry (HHD), the ALS Functional Rating Scale‐Revised (ALSFRS‐R) score, and motor unit number estimation (MUNE). Results: Moderate strength correlations between EIM parameters and HHD were observed for both whole‐body and individual upper and lower extremity values. Similarly, moderate strength correlations were observed between EIM and ALSFRS‐R upper and lower extremity subscores, but not total ALSFRS‐R scores. MUNE correlated significantly with single muscle EIM data but not with whole body or upper or lower extremity values. Conclusions: These results support the concept that EIM can serve as a meaningful measure of disease severity in ALS. Muscle Nerve 49:441–443, 2014
A new pitfall in a sensory conduction study of the lateral antebrachial cutaneous nerve: Spread to the radial nerve
Authors: Oishi Chizuko , Sonoo Masahiro , Kurono Hiroko , Hatanaka Yuki , Shimizu Teruo , Chiba Atsuro , Sakuta Manabu 
Published: November 2013

ABSTRACTIntroduction: We describe a previously unreported pitfall, spread of the stimulus at the elbow to the radial nerve, in an antidromic sensory nerve conduction study of the lateral antebrachial cutaneous (LAC) nerve. Methods: Subjects consisted of 80 healthy volunteers, and both sides were examined for each subject. Besides routine recording of the LAC nerve, sensory nerve action potentials (SNAPs) of the radial nerve were recorded distally. Results: The spread phenomenon occurred in 73 of 160 arms (46%), and the SNAP amplitude increased due to contamination of the radial SNAP up to 6.7 times the genuine LAC SNAP. In 10 arms (6%), the spread started before the LAC SNAP was saturated, and the genuine LAC SNAP was unknown due to an anatomical variation in at least 1 arm. Conclusions: Without monitoring distal radial SNAPs, the spread phenomenon will remain unrecognized. This pitfall undermines the reliability of the test. Muscle Nerve 50:186–192, 2014
Etifoxine provides benefits in nerve repair with acellular nerve grafts
Authors: Zhou Xiang , He Bo , Zhu Zhaowei , He Xinhua , Zheng Canbin , Xu Jian , Jiang Li , Gu Liqiang , Zhu Jiakai , Zhu Qingtang , Liu Xiaolin 
Published: November 2013

ABSTRACTIntroduction: Acellular nerve grafts are good candidates for nerve repair, but the clinical outcome of grafting is not always satisfactory. We investigated whether etifoxine could enhance nerve regeneration. Methods: Seventy‐two Sprague‐Dawley rats were divided into 3 groups: (1) autograft; (2) acellular nerve graft; and (3) acellular nerve graft plus etifoxine. Histological and electrophysiological examinations were performed to evaluate the efficacy of nerve regeneration. Walking‐track analysis was used to examine functional recovery. Quantitative polymerase chain reaction was used to evaluate changes in mRNA level. Results: Etifoxine: (i) increased expression of neurofilaments in regenerated axons; (ii) improved sciatic nerve regeneration measured by histological examination; (iii) increased nerve conduction velocity; (iv) improved walking behavior as measured by footprint analysis; and (v) boosted expression of neurotrophins. Conclusions: These results show that etifoxine can enhance peripheral nerve regeneration across large nerve gaps repaired by acellular nerve grafts by increasing expression of neurotrophins. Muscle Nerve 50:235–243, 2014
On Fröhlich's solution for Boussinesq's problem
Authors: Selvadurai A. P. S. 
Published: November 2013

SUMMARYThe concentration factor introduced by O. K. Fröhlich is visualized as a procedure for examining the pattern of load transfer from surface loads to the interior of a geomaterial. The historical details that led to the introduction of the concentration factor are scant although it is widely used in the area of soil mechanics problems associated with tillage‐induced soil compaction. The purpose of this note is to examine the concentration factor in terms of the geomechanics of an elastic continuum and to identify the precise conditions that are satisfied by the distribution of stresses and strains that accommodate the concentration factor. Copyright © 2013 John Wiley & Sons, Ltd.
Novel LEPR mutations in obese Pakistani children identified by PCR‐based enrichment and next generation sequencing
Authors: Saeed Sadia , Bonnefond Amélie , Manzoor Jaida , Philippe Julien , Durand Emmanuelle , Arshad Mohsin , Sand Olivier , Butt Taeed A , Falchi Mario , Arslan Muhammad , Froguel Philippe 
Published: November 2013

ObjectiveMutations in leptin receptor gene (LEPR) result in early onset extreme adiposity. However, their prevalence in different populations is not known. Indeed, LEPR screening by gold standard Sanger sequencing has been limited by its large size and the cost. One‐step PCR‐based targeted enrichment could be an option for rapid and cost effective molecular diagnosis of monogenic forms of obesity.MethodsThe study is based on 39 unrelated severely obese Pakistani children, previously shown to be negative for leptin (LEP) and melanocortin 4 receptor (MC4R) gene mutations, from an initial cohort of 62 probands. Patient samples were analyzed by microdroplet PCR‐enrichment (RainDance technologies) targeting coding exons of 26 obesity‐associated genes combined with next generation sequencing. Hormone levels were analyzed by ELISA.ResultsThe analysis revealed two novel homozygous LEPR mutations, an essential splice site mutation in exon 15 (c.2396‐1 G>T), and a nonsense mutation in exon 10 (c.1675 G>A). Both probands had high leptin levels and were phenotypically indistinguishable from age‐matched leptin‐deficient subjects from the same population.ConclusionsThe two subjects carrying homozygous LEPR mutations, reported here for the first time in the Pakistani population, constitute 3% of the whole cohort of severely obese children (compared to 17% for LEP and 3% for MC4R).
Oncostatin M receptor is a novel therapeutic target in cervical squamous cell carcinoma
Authors: Caffarel Maria M , Coleman Nicholas 
Published: November 2013

AbstractCervical carcinoma is the second most common cause of cancer deaths in women worldwide. Treatments have not changed for decades and survival rates for advanced disease remain low. An exciting new molecular target for the treatment of cervical squamous cell carcinoma (SCC), and possibly for SCCs at other anatomical sites, is the oncostatin M receptor (OSMR). This cell surface cytokine receptor is commonly copy number gained and overexpressed in advanced cervical SCC, changes that are associated with significantly worse clinical outcomes. OSMR overexpression in cervical SCC cells results in enhanced responsiveness to the major ligand oncostatin M (OSM), which induces several pro‐malignant effects, including a pro‐angiogenic phenotype and increased cell migration and invasiveness. OSMR is a strong candidate for antibody‐mediated inhibition, a strategy that has had a major impact on haematological malignancies and various solid tumours such as HER2‐positive breast cancers. © 2013 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of Pathological Society of Great Britain and Ireland.
SpliceProt: A protein sequence repository of predicted human splice variants
Authors: Tavares Raphael , de Miranda Scherer Nicole , Pauletti Bianca Alves , Araújo Elói , Folador Edson Luiz , Espindola Gabriel , Ferreira Carlos Gil , Paes Leme Adriana Franco , de Oliveira Paulo Sergio Lopes , Passetti Fabio 
Published: November 2013

The mechanism of alternative splicing in the transcriptome may increase the proteome diversity in eukaryotes. In proteomics, several studies aim to use protein sequence repositories to annotate MS experiments or to detect differentially expressed proteins. However, the available protein sequence repositories are not designed to fully detect protein isoforms derived from mRNA splice variants. To foster knowledge for the field, here we introduce SpliceProt, a new protein sequence repository of transcriptome experimental data used to investigate for putative splice variants in human proteomes. Current version of SpliceProt contains 159 719 non‐redundant putative polypeptide sequences. The assessment of the potential of SpliceProt in detecting new protein isoforms resulting from alternative splicing was performed by using publicly available proteomics data. We detected 173 peptides hypothetically derived from splice variants, which 54 of them are not present in UniprotKB/TrEMBL sequence repository. In comparison to other protein sequence repositories, SpliceProt contains a greater number of unique peptides and is able to detect more splice variants. Therefore, SpliceProt provides a solution for the annotation of proteomics experiments regarding splice isofoms. The repository files containing the translated sequences of the predicted splice variants and a visualization tool are freely available at http://lbbc.inca.gov.br/spliceprot.
The C‐terminal domain of the transcriptional regulator BldD from Streptomyces coelicolor A3(2) constitutes a novel fold of winged‐helix domains
Authors: Kim Jeong‐Mok , Won Hyung‐Sik , Kang Sa‐Ouk 
Published: November 2013

ABSTRACTBldD regulates transcription of key developmental genes in Streptomyces coelicolor. While the N‐terminal domain is responsible for both dimerization and DNA binding, the structural and functional roles of the C‐terminal domain (CTD) remain largely unexplored. Here, the solution structure of the BldD‐CTD shows a novel winged‐helix domain fold not compatible with DNA binding, due to the negatively charged surface and presence of an additional helix. Meanwhile, a small elongated groove with conserved hydrophobic patches surrounded by charged residues suggests that the BldD‐CTD could be involved in protein–protein interactions that provide transcriptional regulation. Proteins 2014; 82:1093–1098. © 2013 Wiley Periodicals, Inc.
Invasion of the Q2 mitochondrial variant of Mediterranean Bemisia tabaci in southern Italy: possible role of bacterial endosymbionts
Authors: Parrella Giuseppe , Nappo Anna Giulia , Manco Elena , Greco Barbara , Giorgini Massimo 
Published: November 2013

AbstractBACKGROUNDThe whitefly Bemisia tabaci (Gennadius) is a complex of cryptic species, some of which, namely the Mediterranean (MED) and the Middle East–Asia Minor 1 (MEAM1), are highly invasive and injurious crop pests worldwide and able to displace local genotypes. Invasiveness of B. tabaci may depend on the phenotype of inherited bacterial endosymbionts. Here, the B. tabaci genetic diversity variation that has occurred in recent years in southern Italy was examined. Whitefly was genotyped by restriction fragment length polymorphism analysis of polymerase‐chain‐reaction‐amplified fragments (PCR‐RFLP) of the COI gene and molecular identification of endosymbionts. Possible factors leading to the observed genetic diversity were examined.RESULTSQ1 and Q2 mitochondrial types of MED, the only species found, coexisted in the field, while MEAM1 disappeared. A large spreading of Q2 (70% of individuals) was observed for the first time in Italy. Q2 showed a significant female‐biased sex ratio and largely outnumbered Q1 on solanaceous hosts, in greenhouses and on insecticide‐treated plants. Q1, with an even sex ratio, slightly prevailed on non‐solanaceous hosts, especially on wild and untreated plants. Endosymbiont composition was associated with the mitochondrial type. Hamiltonella and Rickettsia were found at near fixation in Q1 and Q2 respectively; Arsenophonus, Cardinium and Wolbachia were found in both types, although at different frequencies.CONCLUSIONSQ2 invasion seems to have been favoured by the agroecological conditions of southern Italy and by the female‐biased sex ratio. Endosymbionts may have a role in Q2 invasiveness, acting as sex‐ratio manipulators (e.g. Rickettsia) and possibly by benefiting the host fitness. © 2013 Society of Chemical Industry