bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue Oxygen Demand in Regulation of the Behavior of the Cells in the Vasculature
Authors: Barvitenko Nadezhda N. , Aslam Muhammad , Filosa Jessica , Matteucci Elena , Nikinmaa Mikko , Pantaleo Antonella , Saldanha Carlota , Baskurt Oguz K. 
Published: February 2013

AbstractThe control of arteriolar diameters in microvasculature has been in the focus of studies on mechanisms matching oxygen demand and supply at the tissue level. Functionally, important vascular elements include EC, VSMC, and RBC. Integration of these different cell types into functional units aimed at matching tissue oxygen supply with tissue oxygen demand is only achieved when all these cells can respond to the signals of tissue oxygen demand. Many vasoactive agents that serve as signals of tissue oxygen demand have their receptors on all these types of cells (VSMC, EC, and RBC) implying that there can be a coordinated regulation of their behavior by the tissue oxygen demand. Such functions of RBC as oxygen carrying by Hb, rheology, and release of vasoactive agents are considered. Several common extra‐ and intracellular signaling pathways that link tissue oxygen demand with control of VSMC contractility, EC permeability, and RBC functioning are discussed.
Defence responses regulated by jasmonate and delayed senescence caused by ethylene receptor mutation contribute to the tolerance of petunia to Botrytis cinerea
Authors: Wang Hong , Liu Gang , Li Chunxia , Powell Ann L. T. , Reid Michael S. , Zhang Zhen , Jiang Cai‐Zhong 
Published: February 2013

SummaryEthylene and jasmonate (JA) have powerful effects when plants are challenged by pathogens. The inducible promoter‐regulated expression of the Arabidopsis ethylene receptor mutant ethylene‐insensitive1‐1 (etr1‐1) causes ethylene insensitivity in petunia. To investigate the molecular mechanisms involved in transgenic petunia responses to Botrytis cinerea related to the ethylene and JA pathways, etr1‐1‐expressing petunia plants were inoculated with Botrytis cinerea. The induced expression of etr1‐1 by a chemical inducer dexamethasone resulted in retarded senescence and reduced disease symptoms on detached leaves and flowers or intact plants. The extent of decreased disease symptoms correlated positively with etr1‐1 expression. The JA pathway, independent of the ethylene pathway, activated petunia ethylene response factor (PhERF) expression and consequent defence‐related gene expression. These results demonstrate that ethylene induced by biotic stress influences senescence, and that JA in combination with delayed senescence by etr1‐1 expression alters tolerance to pathogens.
Variations in type III effector repertoires, pathological phenotypes and host range of Xanthomonas citri pv. citri pathotypes
Authors: Escalon Aline , Javegny Stéphanie , Vernière Christian , Noël Laurent D. , Vital Karine , Poussier Stéphane , Hajri Ahmed , Boureau Tristan , Pruvost Olivier , Arlat Matthieu , Gagnevin Lionel 
Published: February 2013

SummaryThe mechanisms determining the host range of Xanthomonas are still undeciphered, despite much interest in their potential roles in the evolution and emergence of plant pathogenic bacteria. Xanthomonas citri pv. citri (Xci) is an interesting model of host specialization because of its pathogenic variants: pathotype A strains infect a wide range of Rutaceous species, whereas pathotype A*/AW strains have a host range restricted to Mexican lime (Citrus aurantifolia) and alemow (Citrus macrophylla). Based on a collection of 55 strains representative of Xci worldwide diversity assessed by amplified fragment length polymorphism (AFLP), we investigated the distribution of type III effectors (T3Es) in relation to host range. We examined the presence of 66 T3Es from xanthomonads in Xci and identified a repertoire of 28 effectors, 26 of which were shared by all Xci strains, whereas two (xopAG and xopC1) were present only in some A*/AW strains. We found that xopAG (=avrGf1) was present in all AW strains, but also in three A* strains genetically distant from AW, and that all xopAG‐containing strains induced the hypersensitive response (HR) on grapefruit and sweet orange. The analysis of xopAD and xopAG suggested horizontal transfer between X. citri pv. bilvae, another citrus pathogen, and some Xci strains. A strains were genetically less diverse, induced identical phenotypic responses and possessed indistinguishable T3E repertoires. Conversely, A*/AW strains exhibited a wider genetic diversity in which clades correlated with geographical origin and T3E repertoire, but not with pathogenicity, according to T3E deletion experiments. Our data outline the importance of taking into account the heterogeneity of Xci A*/AW strains when analysing the mechanisms of host specialization.
Fusarium keratitis in South India: causative agents, their antifungal susceptibilities and a rapid identification method for the Fusarium solani species complex
Authors: Homa Mónika , Shobana Coimbatore S. , Singh Yendrembam R. B. , Manikandan Palanisamy , Selvam Kanesan P. , Kredics László , Narendran Venkatapathy , Vágvölgyi Csaba , Galgóczy László 
Published: February 2013

SummarySeventy Fusarium isolates derived from human keratomycosis were identified based on partial sequences of the β‐tubulin (β‐TUB) and translation elongation factor 1α (EF‐1α) genes. Most of the isolates were confirmed as members of the F. solani species complex (75.71%), followed by the F. dimerum species complex (8.57%), the F. fujikuroi species complex (8.57%), the F. oxysporum species complex (4.29%) and the F. incarnatum‐equiseti species complex (2.86%). A combined phylogenetic tree was estimated including all the 70 isolates. Isolates belonging to different species complexes formed separate clades. In this study, we also report the first isolation of F. napiforme from human keratomycosis. A new method based on a specific EcoRI restriction site in the EF‐1α gene was developed for the rapid identification of F. solani. In vitro antifungal susceptibilities of the isolates to seven antifungals were determined by broth microdilution method. Terbinafine, natamycin and amphotericin B proved to be the most effective drugs, followed by voriconazole. The minimal inhibitory concentrations of clotrimazole, econazole and itraconazole were generally high (≥64 μg ml−1). The interactions between the two most effective antifungals (natamycin and terbinafine) were determined by checkerboard microdilution method. Synergism (71.8%) or no interaction (28.2%) was revealed between the two compounds.
Invasive fungal infections in paediatric acute myeloid leukaemia
Authors: Johnston D. L. , Lewis V. , Yanofsky R. , Gillmeister B. , Ethier M. C. , Mitchell D. , Cellot S. , Dix D. , Portwine C. , Price V. , Silva M. , Zelcer S. , Michon B. , Bowes L. , Stobart K. , Brossard J. , Beyene J. , Sung L. 
Published: February 2013

SummaryInvasive fungal infections (IFIs) are a major cause of morbidity and mortality in paediatric acute myeloid leukaemia (AML). This study describes risk factors for IFI and IFI‐related sepsis in this population. We conducted a population‐based, retrospective cohort study of children with AML in Canada. IFIs during chemotherapy and prior to haematopoietic stem cell transplantation, relapse, persistent disease or death were identified. Risk factors for proven or probable IFI were examined. Among courses complicated by IFI, risk factors for sepsis were also evaluated. There were 341 children with AML included of which 41 (12.0%) experienced 46 different episodes of IFI. Candida species accounted for 23 (50.0%) of IFIs and Aspergillus spp. accounted for 14 (30.4%). Days of broad‐spectrum antibiotics, days of corticosteroids and neutropenia at start of the course were independently associated with IFI. Only days of fever were independently associated with IFI‐related sepsis. Invasive fungal infections occurred in 12.0% of paediatric AML patients. Risk factors for IFI and IFI‐related sepsis were identified. This knowledge may help to consider targeted strategies.
The Mediterranean red alga Asparagopsis taxiformis has antifungal activity against Aspergillus species
Authors: Genovese Giuseppa , Leitner Sandra , Minicante Simona A. , Lass‐Flörl Cornelia 
Published: February 2013

SummaryThe red algae Asparagopsis taxiformis collected from the Straits of Messina (Italy) were screened for antifungal activity against Aspergillus species. EUCAST methodology was applied and extracts showed antifungal activity against A. fumigatus, A. terreus and A. flavus. The lowest minimum inhibitory concentrations observed were <0.15 mg ml−1 and the highest were >5 mg ml−1 for Aspergillus spp. tested. Agar diffusion assays confirmed antifungal activity of A. taxiformis extracts in Aspergillus species.
Wechsler Intelligence Scale for Children 4th edition‐Chinese version index scores in Taiwanese children with attention‐deficit/hyperactivity disorder
Authors: Yang Pinchen , Cheng Chung‐Ping , Chang Chen‐Lin , Liu Tai‐Ling , Hsu Hsiu‐Yi , Yen Cheng‐Fang 
Published: February 2013

AimThe Wechsler Intelligence Scale for Children 4th edition‐Chinese version (WISC‐IV‐Chinese) has been in clinical use in Taiwan since 2007. Research is needed to determine how the WISC‐IV, modified from its earlier version, will affect its interpretation in clinical practice in a Mandarin‐speaking context.MethodsWe attempted to use WISC‐IV‐Chinese scores to identify the cognitive strengths and weaknesses in 334 Taiwanese children with attention‐deficit/hyperactivity disorder (ADHD). Comparison of cognitive profiles of WISC‐IV‐Chinese scores between subtypes of ADHD was also performed.ResultsThe results indicated that the four‐factor model of the WISC‐IV‐Chinese fitted well for Taiwanese children with ADHD. The profiles showed that performance in the index score of the Processing Speed Index was the weakness domain for the Taiwanese children with ADHD, as confirmed by two different kinds of analytic methods. Cognitive profile analysis of ADHD subtypes revealed children with inattentive subtypes to have a greater weakness in processing speed performance.ConclusionThe implications of the profiles of the index scores on the WISC‐IV‐Chinese version for Taiwanese children with ADHD were explored.
Study of the efficacy and safety of switching from risperidone to paliperidone in elderly patients with schizophrenia
Authors: Suzuki Hidenobu , Gen Keishi , Otomo Masahiro , Inoue Yuichi , Hibino Hiroyuki , Mikami Ayako , Matsumoto Hideo , Mikami Katsunaka 
Published: February 2013

AimWe investigated the clinical efficacy and safety of switching to paliperidone (PAL) in elderly schizophrenia patients receiving risperidone.MethodsThe subjects were 27 inpatients who had been diagnosed with schizophrenia according to the DSM‐IV. The patient's clinical symptoms were assessed using the Positive and Negative Syndrome Scale and the Clinical Global Impression‐Severity of Illness Scale, and their safety was assessed using the Drug‐induced Extrapyramidal Symptoms Scale, bodyweight, body mass index, and laboratory tests. We also investigated patient satisfaction using the Drug Attitude Inventory, a subjective outcome measure.ResultsNo significant differences in clinical symptom improvement efficacy were seen between the PAL‐switching group and the control group. The mean changes from baseline on the Drug‐induced Extrapyramidal Symptoms Scale total score, Drug Attitude Inventory score, and prolactin level were significantly greater in the PAL‐switching group than in the control group. Furthermore, patients with PAL needed less biperiden, even though they had similar risperidone‐equivalent daily dosages to the control group.ConclusionsThe results of this study suggest that switching elderly patients from risperidone to PAL may result in superior safety and patient satisfaction, and may also make it possible to reduce the dosage of biperiden.
Fetus Papyraceus: Congenital Pulmonary Anomalies Associated with Congenital Aplasia Cutis on the Surviving Twin
Authors: Louise Lagier , Annabel Maruani , Hubert Lardy , Isabelle Gibertini , Gerard Lorette 
Published: February 2013

AbstractAplasia cutis congenita (ACC) can be associated with fetus papyraceus. We report here the first case of ACC linked to fetus papyraceus with pulmonary anomalies. At birth, the patient presented with skin lesions of the trunk consisting of well‐defined, symmetrically distributed, bilateral atrophic ulcerations. Physical examination was otherwise normal. Persistent bronchospasm occurred at the age of 7 months; computed tomography images showed small bilateral pulmonary bullae. At the age of 5 years, skin and pulmonary lesions had not extended. Although the mechanisms of ACC linked to fetus papyraceus are unclear, vascular ischemia is strongly suggested, and could explain the bilateral and symmetric congenital skin and lung aplasia.
Midchildhood Acne Associated with Inhaled Corticosteroids: Report of Two Cases and Review of the Literature
Authors: Liu Kristina J. , Antaya Richard J. 
Published: February 2013

AbstractMidchildhood acne has been attributed to a number of causes in the literature, including adrenocortical tumor, hyperandrogenemia due to hypothalamic dysfunction, and contact with greasy topical skin care products. There are only a few case reports of inhaled steroids causing acneiform eruptions, all of which occurred in adults and with symptoms suggesting that the acne resulted from systemic absorption. We present two cases of comedonal and inflammatory midchildhood acne temporally associated with the use of inhaled corticosteroids administered through face masks, implicating a causative relationship between topical steroid exposure and midchildhood acne that does not necessitate systemic absorption.
Childhood Lichen Sclerosus—A Challenge for Clinicians
Authors: Lagerstedt Maria , Karvinen Kaisa , Joki‐Erkkilä Minna , Huotari‐Orava Riitta , Snellman Erna , Laasanen Satu‐Leena 
Published: February 2013

AbstractChildhood lichen sclerosus (LS) is a rare and often misdiagnosed inflammatory dermatitis with an unpredictable course. The complications of LS are architectural changes of the vulva; malignant transformation is possible. The objective of our study was to define the background and the long‐term course of childhood LS. A registery study identified 44 children with LS treated at Tampere University Hospital, Tampere, Finland, from 1982 to 2010. A questionnaire was sent to the identified patients and 15 responded. The clinical depiction of LS varied significantly. LS was diagnosed in only 16% of the patients at the referring unit. Autoimmune disorders were observed in 6 of the 44 patients. High prevalences of Turner's syndrome (2/44) and kidney disease (2/44) were noted. The majority of the patients were treated with topical corticosteroids. Eight developed architectural changes of the vulva. The questionnaire revealed that three of six patients who were asymptomatic at the end of the registery study follow‐up experienced a recurrence of symptoms. None of them were undergoing follow‐up. Nine of the 15 patients reported reduced quality of life. Childhood LS is a heterogeneous disease with a remarkable effect on quality of life. The misdiagnosis of childhood LS is common. The association between LS and autoimmune diseases should be noted. The high prevalence of Turner's syndrome raises questions regarding the influence of low estrogen levels on the development of LS. The prognosis cannot be predicted, so long‐term follow‐up is recommended. New tools for diagnosis and surveillance are needed.
Reducing Biotic and Abiotic Land‐Use Legacies to Restore Invaded, Abandoned Citrus Groves
Authors: Weiler Annalisa , Von Holle Betsy , Nickerson David M. 
Published: February 2013

AbstractLand‐use legacies associated with agriculture, such as increased soil fertility and elevated soil pH, promote invasions by non‐native plant species on former agricultural lands. Restoring natural soil conditions (i.e. low fertility and low pH) may be an effective, long‐term method to control and reduce the abundance of non‐native and ruderal species that invade abandoned agricultural lands. In this study, we examined how soil manipulation treatments of lowering soil fertility with carbon additions and lowering soil pH by applying sulfur affect non‐native and ruderal native plant species abundance in two former citrus groves in central Florida. Non‐native plant biomass was removed by one of two methods (tilling or topsoil removal), and was combined with a soil amendment of sulfur, carbon, sulfur + carbon, or none. The biomass removal treatments significantly decreased non‐native abundance, with topsoil removal as the most effective. Carbon additions did not affect soil fertility or vegetation. Sulfur and sulfur + carbon additions significantly decreased soil pH in both groves for at least 1 year post‐treatment; however, we did not see a significant vegetation response. Overall, our results suggest that removing vegetation by tilling and topsoil removal is an effective method for reducing non‐target species cover. Although we did not see a response of vegetation to our treatments, we were able to restore the initial soil characteristics, which can be a first step toward complete restoration.
Artificial Spawning Habitats Improve Egg Production of a Declining Diadromous Fish, Galaxias maculatus (Jenyns, 1842)
Authors: Hickford Michael J. H. , Schiel David R. 
Published: February 2013

AbstractAnthropogenic impacts from urbanization, deforestation, and agriculture have degraded the riparian margins of waterways worldwide. In New Zealand, such impacts have caused changes in native vegetation, enhanced invasion by exotic grasses, and altered river bank morphology. One consequence has been a great reduction in obligate spawning habitat of a diadromous fish, Galaxias maculatus. Juvenile G. maculatus comprise a culturally important fishery that has been considerably reduced over recent decades. Rehabilitation of riparian vegetation needed for spawning is relatively straightforward, but slow. We hypothesized that artificial spawning habitats could accelerate restoration of fish egg production by creating an environment that would support at least the same density and survival of eggs as non‐impacted vegetation. We tested three artificial devices (straw bales, straw tubes, and moss tubes) in degraded and intact sites. Eggs were laid in all of these with numbers and survival usually exceeding that in riparian grasses. Where habitat was degraded, artificial spawning habitats yielded up to 10,000 eggs compared to none in nearby natural spawning habitat. The ground‐level environment of artificial habitat was similar to that of intact vegetation in buffering ambient temperature and humidity fluctuations. Crucial properties of the artificial habitats were (1) shelter to provide shade and hold moisture; (2) accessibility to allow adult fish to deposit and fertilize eggs; and (3) robustness to provide reliable surfaces and protection for the eggs during their development. We show that artificial spawning habitats are a viable short‐term alternative to rehabilitating spawning habitat while legacy effects abate and riparian vegetation recovers.
Effect of health professional education on outcomes of chronic obstructive pulmonary disease in primary care: A non randomized clinical trial
Authors: Monteagudo Mònica , Rodriguez‐Blanco Teresa , Llagostera Maria , Valero Carles , Bayona Xavier , Granollers Sílvia , Ferro Juan J. , Acedo Mateo , Almeda Jesús , Rodriguez‐Latre Lluisa , Muñoz Laura , Miravitlles Marc 
Published: February 2013

AbstractBackground and objectiveThe best strategy to achieve optimal integrated management to patients with chronic obstructive pulmonary disease (COPD) in primary care (PC) is not clear. We aimed to evaluate the effectiveness of an intervention in PC based on an integrated programme, which combines diverse strategies directed at health professionals to improve quality of life and clinical outcomes of their patients with COPD. We compared the outcome with results from standard practice.MethodsA prospective, multicentre, quasi‐experimental study and a 12‐month follow up was performed. Intervention consisted of an integrated education programme in PC. The main outcome variable was the change in total score of the St. George's Respiratory Questionnaire (SGRQ) at the end of follow up between the control and intervention group.ResultsA total of 801 patients participated in the study with a mean age of 70.2 years and a mean FEV1 (% predicted) of 55%. At 1‐year follow up, the SGRQ score did not significantly differ. Although the intervention group showed an improvement in dietary, exercise and smoking habits, there was an increase in reported exacerbations and hospital admissions (P < 0.001).ConclusionsImplementation of an integrated education programme in a PC setting, which combines diverse strategies directed at health professionals, did not achieve the expected changes in quality of life measured by SGRQ at 1‐year follow up. Nonetheless, this study observed an improvement in patients’ lifestyle choices, even though this did not result in a significant change in the clinical evolution or heath status over 12 months.
Automatic 3D segmentation of multiphoton images: a key step for the quantification of human skin
Authors: Decencière Etienne , Tancrède‐Bohin Emmanuelle , Dokládal Petr , Koudoro Serge , Pena Ana‐Maria , Baldeweck Thérèse 
Published: February 2013

Background/purposeMultiphoton microscopy has emerged in the past decade as a useful noninvasive imaging technique for in vivo human skin characterization. However, it has not been used until now in evaluation clinical trials, mainly because of the lack of specific image processing tools that would allow the investigator to extract pertinent quantitative three‐dimensional (3D) information from the different skin components.MethodsWe propose a 3D automatic segmentation method of multiphoton images which is a key step for epidermis and dermis quantification. This method, based on the morphological watershed and graph cuts algorithms, takes into account the real shape of the skin surface and of the dermal–epidermal junction, and allows separating in 3D the epidermis and the superficial dermis.ResultsThe automatic segmentation method and the associated quantitative measurements have been developed and validated on a clinical database designed for aging characterization. The segmentation achieves its goals for epidermis–dermis separation and allows quantitative measurements inside the different skin compartments with sufficient relevance.ConclusionsThis study shows that multiphoton microscopy associated with specific image processing tools provides access to new quantitative measurements on the various skin components. The proposed 3D automatic segmentation method will contribute to build a powerful tool for characterizing human skin condition. To our knowledge, this is the first 3D approach to the segmentation and quantification of these original images.