bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thoracic aorta 3D hemodynamics in pediatric and young adult patients with bicuspid aortic valve
Authors: Allen Bradley D. , van Ooij Pim , Barker Alex J. , Carr Maria , Gabbour Maya , Schnell Susanne , Jarvis Kelly B. , Carr James C. , Markl Michael , Rigsby Cynthia , Robinson Joshua D. 
Published: January 2015

BackgroundTo evaluate the 3D hemodynamics in the thoracic aorta of pediatric and young adult bicuspid aortic valve (BAV) patients.Methods4D flow MRI was performed in 30 pediatric and young adult BAV patients (age: 13.9 ± 4.4 (range: [3.4, 20.7]) years old, M:F = 17:13) as part of this Institutional Review Board‐approved study. Nomogram‐based aortic root Z‐scores were calculated to assess aortic dilatation and degree of aortic stenosis (AS) severity was assessed on MRI. Data analysis included calculation of time‐averaged systolic 3D wall shear stress (WSSsys) along the entire aorta wall, and regional quantification of maximum and mean WSSsys and peak systolic velocity (velsys) in the ascending aorta (AAo), arch, and descending aorta (DAo). The 4D flow MRI AAo velsys was also compared with echocardiography peak velocity measurements.ResultsThere was a positive correlation with both mean and max AAo WSSsys and peak AAo velsys (mean: r = 0.84, P < 0.001, max: r = 0.94, P < 0.001) and AS (mean: rS = 0.43, P = 0.02, max: rS = 0.70, P < 0.001). AAo peak velocity was significantly higher when measured with echo compared with 4D flow MRI (2.1 ± 0.98 m/s versus 1.27 ± 0.49 m/s, P < 0.001).ConclusionIn pediatric and young adult patients with BAV, AS and peak ascending aorta velocity are associated with increased AAo WSS, while aortic dilation, age, and body surface area do not significantly impact AAo hemodynamics. Prospective studies are required to establish the role of WSS as a risk‐stratification tool in these patients. J. Magn. Reson. Imaging 2015;42:954–963.
In vivo visualization and ex vivo quantification of murine breast cancer cells in the mouse brain using MRI cell tracking and electron paramagnetic resonance
Authors: Danhier Pierre , Magat Julie , Levêque Philippe , De Preter Géraldine , Porporato Paolo E. , Bouzin Caroline , Jordan Bénédicte F. , Demeur Gladys , Haufroid Vincent , Feron Olivier , Sonveaux Pierre , Gallez Bernard 
Published: January 2015

Cell tracking could be useful to elucidate fundamental processes of cancer biology such as metastasis. The aim of this study was to visualize, using MRI, and to quantify, using electron paramagnetic resonance (EPR), the entrapment of murine breast cancer cells labeled with superparamagnetic iron oxide particles (SPIOs) in the mouse brain after intracardiac injection.For this purpose, luciferase‐expressing murine 4 T1‐luc breast cancer cells were labeled with fluorescent Molday ION Rhodamine B SPIOs. Following intracardiac injection, SPIO‐labeled 4 T1‐luc cells were imaged using multiple gradient‐echo sequences. Ex vivo iron oxide quantification in the mouse brain was performed using EPR (9 GHz). The long‐term fate of 4 T1‐luc cells after injection was characterized using bioluminescence imaging (BLI), brain MRI and immunofluorescence.We observed hypointense spots due to SPIO‐labeled cells in the mouse brain 4 h after injection on T2*‐weighted images. Histology studies showed that SPIO‐labeled cancer cells were localized within blood vessels shortly after delivery. Ex vivo quantification of SPIOs showed that less than 1% of the injected cells were taken up by the mouse brain after injection. MRI experiments did not reveal the development of macrometastases in the mouse brain several days after injection, but immunofluorescence studies demonstrated that these cells found in the brain established micrometastases. Concerning the metastatic patterns of 4 T1‐luc cells, an EPR biodistribution study demonstrated that SPIO‐labeled 4 T1‐luc cells were also entrapped in the lungs of mice after intracardiac injection. BLI performed 6 days after injection of 4 T1‐luc cells showed that this cell line formed macrometastases in the lungs and in the bones.Conclusively, EPR and MRI were found to be complementary for cell tracking applications. MRI cell tracking at 11.7 T allowed sensitive detection of isolated SPIO‐labeled cells in the mouse brain, whereas EPR allowed the assessment of the number of SPIO‐labeled cells in organs shortly after injection. Copyright © 2015 John Wiley & Sons, Ltd.
Maximum principle for optimal control of anticipated forward–backward stochastic differential delayed systems with regime switching
Authors: Lv Siyu , Tao Ran , Wu Zhen 
Published: January 2015

SummaryThis paper is concerned with a Pontryagin maximum principle for optimal control problem of stochastic system, which is described by an anticipated forward–backward stochastic differential delayed equation and modulated by a continuous‐time finite‐state Markov chain. We establish a necessary maximum principle and sufficient verification theorem for the optimal control by virtue of the duality method and convex analysis. To illustrate the theoretical results, we apply them to a recursive utility investment‐consumption problem, and the optimal consumption rate is derived explicitly. Copyright © 2015 John Wiley & Sons, Ltd.
Acaricidal activity of compounds from Cinnamomum camphora (L.) Presl against the carmine spider mite, Tetranychus cinnabarinus
Authors: Chen Yijuan , Dai Guanghui 
Published: January 2015

AbstractBACKGROUNDTetranychus cinnabarinus (Boisduval) is one of the most important, highly polyphagous pests of a wide range of field and greenhouse crops throughout the world. The control of this mite is still based primarily on the use of synthetic chemical pesticides. In this study, we screened eight plant extracts from China and evaluated the natural compounds showing acaricidal properties from the plant extract, considering their potential use as an alternative to synthetic pesticides.RESULTSIn bioassay screening assays, the Cinnamomum camphora (L.) Presl extract showed significantly greater acaricidal activity against T. cinnabarinus than the other seven plant extracts tested. Five compounds were identified from the C. camphora extract via repeated column chromatography and gas chromatography–mass spectrometry analysis. All the compounds presented acaricidal activity, with 2,4‐di‐tert‐butylphenol and ethyl oleate exhibiting the greatest activity. At 7 days after treatment in a potted seedling experiment, the LC50 values of 2,4‐di‐tert‐butylphenol and ethyl oleate were found to be 1850.94 and 2481.65 mg kg−1 respectively. Microscopic observations showed that the mites displayed the symptomology of poisoning.CONCLUSIONThese results demonstrated that the C. camphora extract and its two active components show the potential to be developed as new natural acaricides for controlling carmine spider mites. © 2014 Society of Chemical Industry
Processes discriminating adaptive and maladaptive Internet use among European adolescents highly engaged online
Authors: Tzavela Eleni C. , Karakitsou Chryssoula , Dreier Michael , Mavromati Foteini , Wölfling Klaus , Halapi Eva , Macarie George , Wójcik Szymon , Veldhuis Lydian , Tsitsika Artemis K. 
Published: January 2015

ABSTRACTToday adolescents are highly engaged online. Contrary to common concern, not all highly engaged adolescents develop maladaptive patterns of internet use. The present qualitative study explored the experiences, patterns and impact of use of 124 adolescents (Mage = 16.0) reporting signs of internet addictive behaviors. The focus was to discern adaptive and maladaptive use patterns, which promote or interfere with adolescents' development, respectively. Semi‐structured individual interviews were conducted in seven European countries (Greece, Spain, Poland, Germany, Romania, Netherlands and Iceland) and qualitatively analyzed using grounded theory. Considerable variability emerged in the way adolescents satisfied their personal needs online and offline, in the experienced impact from high online engagement and functional value ascribed to the internet, and in the self‐regulatory processes underlying use. Variability in these discriminating processes was linked to adaptive or maladaptive adolescent internet use patterns. The emerged processes can provide direction for designing prevention and intervention programs promoting adaptive use.
Recommended dairy product intake modulates circulating fatty acid profile in healthy adults: a multi-centre cross-over study
Authors: Abdullah Mohammad M. H. , Cyr Audrey , Lépine Marie-Claude , Labonté Marie-Ève , Couture Patrick , Jones Peter J. H. , Lamarche Benoît 
Published: January 2015

Dairy products are rich sources of an array of fatty acids (FA) that have been shown individually and in certain clusters to exert varying effects on cardiovascular health, for which the circulating lipid profile is a powerful biomarker. Whether the profile of these FA is reflected in blood upon short terms of intake, possibly contributing to the lipid-related health impacts of dairy products, remains to be fully established. The objectives of the present study were to assess a recommended dairy product consumption in relation to circulating FA and lipid profiles, and to evaluate certain FA in dairy fat as potential biomarkers of intake. In a free-living, multi-centre, cross-over design, 124 healthy individuals consumed 3 servings/d of commercial dairy (DAIRY; 1 % fat milk, 1·5 % fat yogurt and 34 % fat cheese) or energy-equivalent control (CONTROL; fruit and vegetable juice, cashews and a cookie) products for 4 weeks each, separated by a 4-week washout period. Plasma FA and serum lipid profiles were assessed by standard methods at the end of each dietary phase. After 4 weeks of intake, plasma levels of FA pentadecanoic acid (15 : 0) and heptadecanoic acid (17 : 0) were higher (0·26v.0·22 % and 0·42v.0·39 % of the total identified FA, respectively) after the DAIRY phase than after the CONTROL phase (P< 0·0001). This was accompanied by a small but significant increase in serum LDL-cholesterol levels after the DAIRY phase compared with the CONTROL phase (+0·08 mmol/l;P= 0·04). In conclusion, intake of 3 servings/d of conventional dairy products may modify certain circulating FA and lipid profiles within 4 weeks, where 15 : 0 and 17 : 0 may be potential short-term biomarkers of intake.
From Babylon to Baghdad: Cultural Heritage and Constitutional Law in the Republic of Iraq
Authors: Davis Tess 
Published: January 2015

Abstract:The Constitution of the Republic of Iraq entered force in 2005, placing such “national treasures” as “antiquities, archeological sites, cultural buildings, manuscripts, and coins” under federal jurisdiction to be “managed in cooperation with the regions and governorates.” This provision may not immediately appear significant or controversial, but it is both. Federalism remains a heated and even deadly issue in Iraq, which is still balancing authority between its capital and other parts of the country. The Constitution’s handling of heritage—like its comparable treatment of oil and gas—therefore raises many questions. The answers to these have massive implications, as they not only determine who governs culture in Iraq but also could void much existing domestic law and unravel the country’s entire heritage management system. This study thus aims to clarify the Constitution’s treatment of antiquities and archaeology, resolving who controls one of Iraq’s most important historic, cultural, and economic resources.
The first pathogenicYersinia enterocoliticabioserotype 4/O:3 strain isolated from a hunted wild boar (Sus scrofa) in Poland
Authors: BANCERZ-KISIEL A. , PLATT-SAMORAJ A. , SZCZERBA-TUREK A. , SYCZYŁO K. , SZWEDA W. 
Published: January 2015

SUMMARYThe objective of this study was to identify the bioserotypes and virulence markers ofYersinia enterocoliticastrains isolated from wild boars in Poland. Bacteriological examination of 302 rectal swabs from 151 wild boars resulted in the isolation of 40Y. enterocoliticastrains. The majority of the examined strains (n= 30), belonged to bioserotype 1A/NI. The presence of individualY. enterocoliticastrains belonging to bioserotypes 1B/NI (3), 1A/O:8 (2), 1A/O:27 (2), 2/NI (1), 2/O:9 (1) and 4/O:3 (1) was also demonstrated. Amplicons corresponding toailandystAgenes were observed only in oneY. enterocoliticastrain – bioserotype 4/O:3. TheailandystBgene amplicons were noted in 11Y. enterocoliticabiotype 1A strains, although single amplicons ofystBgene were found in 28 of the tested samples. In four out of eight cases when twoY. enterocoliticastrains were isolated from the same animal, the strains differed in biotype, serotype or virulence markers. The European population of wild boars continues to grow and spread to new areas, therefore, wild boars harbouring potentially pathogenicY. enterocolitica4/O:3 strains pose a challenge to public health.
Vascular chronic Q fever: quality of life
Authors: HAGENAARS J. C. J. P. , WEVER P. C. , SHAMELIAN S. O. A. , VAN PETERSEN A. S. , HILBINK M. , RENDERS N. H. M. , DE JAGER-LECLERCQ G. L. , MOLL F. L. , KONING O. H. J. 
Published: January 2015

SUMMARYThe aim of this study was to evaluate the quality of life in patients with vascular chronic Q fever at time of diagnosis and during follow-up. Based upon the SF-36 questionnaire, the mean physical and mental health of each patient were assessed at 3-month intervals for up to 18 months. A total of 26 patients were included in the study. At time of diagnosis, the mean physical health and mental health score was 50·6 [95% confidence interval (CI) 46·7–54·4] and 44·6 (95% CI 41·6–47·5), respectively. During treatment, the mean physical health score declined significantly by 1·7 points each 3 months (P < 0·001) to 40·8 (95% CI 34·4–45·1). The mean mental health score significantly and steadily increased towards 51·2 (95% CI 46·9–54·3) during follow-up (P = 0·026). A total of 23% of patients were cured after 18 months of follow-up. In conclusion, quality of life at time of diagnosis for patients with vascular chronic Q fever is lower compared to a similar group of patients, matched for age and gender, with an aortic abdominal aneurysmal disease, and physical health decreases further after starting treatment. Considering the low percentage of cure, the current treatment of vascular chronic Q fever patients may require a separate strategy from that of endocarditis in order to increase survival.
Bmi1 regulates auditory hair cell survival by maintaining redox balance
Authors: Chen Y , Li L , Ni W , Zhang Y , Sun S , Miao D , Chai R , Li H 
Published: January 2015

AbstractReactive oxygen species (ROS) accumulation are involved in noise- and ototoxic drug-induced hair cell loss, which is the major cause of hearing loss. Bmi1 is a member of the Polycomb protein family and has been reported to regulate mitochondrial function and ROS level in thymocytes and neurons. In this study, we reported the expression of Bmi1 in mouse cochlea and investigated the role of Bmi1 in hair cell survival. Bmi1 expressed in hair cells and supporting cells in mouse cochlea. Bmi1−/− mice displayed severe hearing loss and patched outer hair cell loss from postnatal day 22. Ototoxic drug-induced hair cells loss dramatically increased in Bmi1−/− mice compared with that in wild-type controls both in vivo and in vitro, indicating Bmi1−/− hair cells were significantly more sensitive to ototoxic drug-induced damage. Cleaved caspase-3 and TUNEL staining demonstrated that apoptosis was involved in the increased hair cell loss of Bmi1−/− mice. Aminophenyl fluorescein and MitoSOX Red staining showed the level of free radicals and mitochondrial ROS increased in Bmi1−/− hair cells due to the aggravated disequilibrium of antioxidant–prooxidant balance. Furthermore, the antioxidant N-acetylcysteine rescued Bmi1−/− hair cells from neomycin injury both in vitro and in vivo, suggesting that ROS accumulation was mainly responsible for the increased aminoglycosides sensitivity in Bmi1−/− hair cells. Our findings demonstrate that Bmi1 has an important role in hair cell survival by controlling redox balance and ROS level, thus suggesting that Bmi1 may work as a new therapeutic target for the prevention of hair cell death.
Age-related proteostasis and metabolic alterations in Caspase-2-deficient mice
Authors: Wilson C H , Shalini S , Filipovska A , Richman T R , Davies S , Martin S D , McGee S L , Puccini J , Nikolic A , Dorstyn L , Kumar S 
Published: January 2015

AbstractAgeing is a complex biological process for which underlying biochemical changes are still largely unknown. We performed comparative profiling of the cellular proteome and metabolome to understand the molecular basis of ageing in Caspase-2-deficient (Casp2−/−) mice that are a model of premature ageing in the absence of overt disease. Age-related changes were determined in the liver and serum of young (6–9 week) and aged (18–24 month) wild-type and Casp2−/− mice. We identified perturbed metabolic pathways, decreased levels of ribosomal and respiratory complex proteins and altered mitochondrial function that contribute to premature ageing in the Casp2−/− mice. We show that the metabolic profile changes in the young Casp2−/− mice resemble those found in aged wild-type mice. Intriguingly, aged Casp2−/− mice were found to have reduced blood glucose and improved glucose tolerance. These results demonstrate an important role for caspase-2 in regulating proteome and metabolome remodelling during ageing.
NF-κB-dependent and -independent epigenetic modulation using the novel anti-cancer agent DMAPT
Authors: Nakshatri H , Appaiah H N , Anjanappa M , Gilley D , Tanaka H , Badve S , Crooks P A , Mathews W , Sweeney C , Bhat-Nakshatri P 
Published: January 2015

AbstractThe transcription factor nuclear factor-kappaB (NF-κB) is constitutively active in several cancers and is a target of therapeutic development. We recently developed dimethylaminoparthenolide (DMAPT), a clinical grade water-soluble analog of parthenolide, as a potent inhibitor of NF-κB and demonstrated in vitro and in vivo anti-tumor activities in multiple cancers. In this study, we show DMAPT is an epigenetic modulator functioning in an NF-κB-dependent and -independent manner. DMAPT-mediated NF-κB inhibition resulted in elevated histone H3K36 trimethylation (H3K36me3), which could be recapitulated through genetic ablation of the p65 subunit of NF-κB or inhibitor-of-kappaB alpha super-repressor overexpression. DMAPT treatment and p65 ablation increased the levels of H3K36 trimethylases NSD1 (KMT3B) and SETD2 (KMT3A), suggesting that NF-κB directly represses their expression and that lower H3K36me3 is an epigenetic marker of constitutive NF-κB activity. Overexpression of a constitutively active p65 subunit of NF-κB reduced NSD1 and H3K36me3 levels. NSD1 is essential for DMAPT-induced expression of pro-apoptotic BIM, indicating a functional link between epigenetic modification and gene expression. Interestingly, we observed enhanced H4K20 trimethylation and induction of H4K20 trimethylase KMT5C in DMAPT-treated cells independent of NF-κB inhibition. These results add KMT5C to the list NF-κB-independent epigenetic targets of parthenolide, which include previously described histone deacetylase 1 (HDAC-1) and DNA methyltransferase 1. As NSD1 and SETD2 are known tumor suppressors and loss of H4K20 trimethylation is an early event in cancer progression, which contributes to genomic instability, we propose DMAPT as a potent pharmacologic agent that can reverse NF-κB-dependent and -independent cancer-specific epigenetic abnormalities.
Interaction of differentiated human adipocytes with macrophages leads to trogocytosis and selective IL-6 secretion
Authors: Sárvári A K , Doan-Xuan Q-M , Bacsó Z , Csomós I , Balajthy Z , Fésüs L 
Published: January 2015

AbstractObesity leads to adipose tissue inflammation that is characterized by increased release of proinflammatory molecules and the recruitment of activated immune cells. Although macrophages are present in the highest number among the immune cells in obese adipose tissue, not much is known about their direct interaction with adipocytes. We have introduced anex vivoexperimental system to characterize the cellular interactions and the profile of secreted cytokines in cocultures of macrophages and human adipocytes differentiated from either mesenchymal stem cells or a preadipocyte cell line. As observed by time-lapse microscopy, flow, and laser-scanning cytometry, macrophages phagocytosed bites of adipocytes (trogocytosis), which led to theirde novo,phagocytosis and NF-κB-dependent synthesis, then release of interleukin (IL)-6 and monocyte chemoattractant protein (MCP)-1. IL-6 secretion was not accompanied by secretion of other proinflammatory cytokines, such as tumor necrosis factor (TNF)-αand IL-8, except MCP-1. LPS-induced release of TNF-α, IL-8 and MCP-1 was decreased in the presence of the differentiated adipocytes but the IL-6 level did not subside suggesting that phagocytosis-dependent IL-6 secretion may have significant regulatory function in the inflamed adipose tissue.
Analyzing large-scale samples confirms the association between rs16892766 polymorphism and colorectal cancer susceptibility
Authors: Liao Mingzhi , Wang Guangyu , Quan Baoku , Qi Xingsi , Yu Zhihui , Feng Rennan , Zhang Liangcai , Jiang Yongshuai , Zhang Yanqiao , Liu Guiyou 
Published: January 2015

AbstractColorectal cancer (CRC) is a common complex disease caused by the combination of genetic variants and environmental factors. Genome-wide association studies (GWAS) have been performed and reported some novel CRC susceptibility variants. The rs16892766 (8q23.3) polymorphism was first identified to be significantly associated with CRC in European ancestry. The following studies investigated this association in Chinese, Japanese, Romanian, Swedish, African American, European American and Croatian populations. These studies reported consistent and inconsistent results. Here, we reevaluated this association using the relatively large-scale samples from 13 studies (N = 59737, 26237 cases and 33500 controls) using a meta-analysis by searching the PubMed, Google Scholar and CRCgene databases. We observed no significant heterogeneity among the included studies. Our results showed significant association between rs16892766 polymorphism and CRC (P = 1.33E-35, OR = 1.23, 95% CI 1.20-1.27). Collectively, our analysis further supports previous findings that the rs16892766 polymorphism is significantly associated with CRC susceptibility. We believe that our findings will be very useful for future genetic studies on CRC.
Predicting and Influencing Voice Therapy Adherence Using Social–Cognitive Factors and Mobile Video
Authors: van Leer Eva , Connor Nadine P. 
Published: January 2015

Purpose
Patient adherence to voice therapy is an established challenge. The purpose of this study was (a) to examine whether adherence to treatment could be predicted from three social–cognitive factors measured at treatment onset: self-efficacy, goal commitment, and the therapeutic alliance, and (b) to test whether the provision of clinician, self-, and peer model mobile treatment videos on MP4 players would influence the same triad of social cognitive factors and the adherence behavior of patients.

Method
Forty adults with adducted hyperfunction with and without benign lesions were prospectively randomized to either 4 sessions of voice therapy enhanced by MP4 support or without MP4 support. Adherence between sessions was assessed through self-report. Social cognitive factors and voice outcomes were assessed at the beginning and end of therapy. Utility of MP4 support was assessed via interviews.

Results
Self-efficacy and the therapeutic alliance predicted a significant amount of adherence variance. MP4 support significantly increased generalization, self-efficacy for generalization, and the therapeutic alliance. An interaction effect demonstrated that MP4 support was particularly effective for patients who started therapy with poor self-efficacy for generalization.

Conclusion
Adherence may be predicted and influenced via social–cognitive means. Mobile technology can extend therapy to extraclinical settings.