bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural regulation and property balancing of ethylene‐propylene‐diene monomer‐based oil‐absorbing expandable rubber modified with flexible α‐olefins
Authors: Zou Jincheng , Liang Junyi , Luo Siyu , Hong Xiaofeng , Yin Kaiju , Fu Yujie , Fan Minmin , Zhang Xi 
Published: August 2024

AbstractTo regulate the comprehensive performance of ethylene‐propylene‐diene monomer (EPDM)‐based oil‐absorbing expandable rubber, a series of irradiated α‐olefin‐EPDM7001 rubbers (EOR7001‐X‐Y) are prepared. The influence of the carbon chain length and content of α‐olefins on the structure and properties of the rubber is systematically investigated. Results show that EOR7001‐X‐Y possesses a cross‐linked network structure, and its gel fraction decreases gradually with the increase in carbon chain length and content of α‐olefins. The incorporation of α‐octadecene successfully creates micro‐nanoscale porous structures within the rubber matrix, providing favorable conditions for oil absorption. The introduction of α‐olefins and changes in irradiation dose do not significantly affect the thermal stability of EPDM7001 rubber. The tensile strength slightly reduces, while elongation at break significantly increases, improving the deformation capability. EOR7001‐18‐20 demonstrates exceptional resistance to aging and withstands external forces even after oil absorption. This study supports the development of high‐performance oil‐absorbing expandable rubber.
Bio‐based poly(trimethylene terephthalate)/polyamide 56 blend fibers via melt spinning: Preparation, structure, and properties
Authors: Wang Aming , Duan Zeping , Yu Dongzheng , Qin Shihua , Liu Qingsheng , Li Dawei , Li Haoxuan , Deng Bingyao , Xu Weilin 
Published: August 2024

AbstractMelt‐spun poly(trimethylene terephthalate) (PTT) and polyamide 56 (PA56) fibers are generally prepared individually. Herein, inspired by the complementary properties of these two materials, PTT/PA56 blend fibers were prepared by two‐step melt spinning and drawing processes. The regularity of crystalline regions, overall orientation in crystalline and amorphous regions, and dimensional stability under hot and humid conditions can be improved by increasing drawing temperature. The most suitable drawing temperature is 90°C, at which 4‐fold drawn PTT and PA56 fibers show tenacity of 2.94 and 3.54 cN/dtex, respectively. An increase in draw ratio leads to increase in the order of crystal regions and overall orientation in crystalline and amorphous phases. Interestingly, increasing dispersed phase content from 0 to 10 wt% is conducive to the increase in tenacity of samples. The PTT/PA56 (10/90) blend fiber suggests the maximum tenacity value, and tenacity of 4‐fold drawn fiber is 4.02 cN/dtex. As PA56 content increases, dimensional stability of samples under dry conditions increases. Comprehensive analysis demonstrates that the PTT/PA56 (10/90) blend fiber achieves the best combination of tensile properties and dimensional stability. The prepared PTT/PA56 blend fibers are potentially competitive in the fields of fibers, garment, and decoration due to their satisfactory comprehensive performances.
Phase equilibrium and mixing thermodynamics of polystyrene with copolymers of styrene and methyl methacrylate
Authors: Nikulova Uliana V. , Chalykh Anatoly E. 
Published: August 2024

AbstractData on the solubility of oligomer polystyrene and random copolymers of styrene and methyl methacrylate with different styrene contents have been obtained. Phase diagrams were constructed and the values of critical points were determined. The upper critical solution temperature is localized in the temperature range above 420 K and grows with increasing molecular weight of PS and decreasing styrene content in the copolymer. Based on the compositions of the coexisting phases, paired interaction parameters were calculated and their temperature dependences were plotted. The dependence of the interaction parameter on the copolymer composition and the correlation dependence between its enthalpy and entropy components were constructed. A method for simulation phase equilibrium for copolymers of arbitrary composition and molecular weight is proposed.
Preparation of polyaniline‐aniline modified silicon dioxide composite with synergistic anticorrosion effect for waterborne epoxy anticorrosive coatings
Authors: Deng Haiyang , Zhang Hong , Li Hongqiang , Chen Zhonghua 
Published: August 2024

AbstractOwing to environmental friendliness, waterborne coatings have attracted considerable attention for the protection of mechanical equipment. Herein, polyaniline (PANI)‐aniline modified silicon dioxide (ANSiO2) composite was prepared to improve the corrosion resistance of waterborne epoxy coatings through the modification of SiO2 nanoparticles by aniline‐methyl‐triethoxysilane and the subsequent in situ polymerization of aniline (AN). When the molar ratio of AN to ANSiO2 was 6:1, the obtained PANI‐ANSiO2 composite appeared spherical‐planar shape and was able to obviously improve the anticorrosive performance of the epoxy coatings. The impedance value of the PANI‐ANSiO2 composite modified coatings maintained above 108 Ω cm2 after being immersed in 3.5 wt% NaCl solution for 30 days, and the surface of the coating had no corrosion spread after 30 days of salt spray erosion test. The excellent anticorrosive effect of the PANI‐ANSiO2 was mainly attributed to not only the filling of the defective parts of the coatings by hydrophobic ANSiO2 but also the formation of passivation layer on the coating surface by PANI to impede electrochemical corrosion reaction. The preparation method in this work is facile, low‐cost, and easy produced on a large scale, and the environment friendly high‐anticorrosion coating shows great application potential in the field of metal protection.
Genetic Diversity and Population Dynamics of Lobodon carcinophaga in Danco Coast, Antarctica
Authors: Prosdocimi Laura , Ibañez Ezequiel Alejandro , Gariboldi María Constanza , Negrete Javier , Bobinac Magalí , Cappozzo Humberto Luis , Túnez Juan Ignacio 
Published: August 2024

ABSTRACTThe crabeater seal (Lobodon carcinophaga) is the most common Antarctic seal; however, it remains understudied regarding genetic aspects. This research examined the genetic diversity and population dynamics of the Danco Coast population in the Antarctic Peninsula and compared it with the Western Antarctica population. Twenty novel haplotypes were identified, revealing a stable population phase and a brief expansion. The high genetic diversity aligns with those found in similar species and environments. Historical population dynamics correlate with Pleistocene climate shifts, shedding light on climate change's impact on Antarctic ecosystems. This study increases our understanding of Antarctic seal species, contributing to the region's guidelines to improve environmental conservation policies in the Antarctic sector.
Cyclization of acyl thiosemicarbazides led to new Helicobacter pylori α‐carbonic anhydrase inhibitors
Authors: Gumus Arzu , D'Agostino Ilaria , Puca Valentina , Crocetta Valentina , Carradori Simone , Cutarella Luigi , Mori Mattia , Carta Fabrizio , Angeli Andrea , Capasso Clemente , Supuran Claudiu T. 
Published: August 2024

AbstractThe eradication of Helicobacter pylori, the etiologic agent of gastric ulcer and adenocarcinoma, is a big concern in clinics due to the increasing drug resistance phenomena and the limited number of efficacious treatment options. The exploitation of the H. pylori carbonic anhydrases (HpCAs) as promising pharmacological targets has been validated by the antibacterial activity of previously reported CA inhibitors due to the role of these enzymes in the bacterium survival in the gastric mucosa. The development of new HpCA inhibitors seems to be on the way to filling the existing antibiotics gap. Due to the recent evidence on the ability of the coumarin scaffold to inhibit microbial α‐CAs, a large library of derivatives has been developed by means of a pH‐regulated cyclization reaction of coumarin‐bearing acyl thiosemicarbazide intermediates. The obtained 1,3,4‐thiadiazoles (10–18a,b) and 1,2,4‐triazole‐3‐thiones (19–26a,b) were found to strongly and selectively inhibit HpαCA and computational studies were fundamental to gaining an understanding of the interaction networks governing the enzyme–inhibitor complex. Antibacterial evaluations on H. pylori ATCC 43504 highlighted some compounds that maintained potency on a resistant clinical isolate. Also, their combinations with metronidazole decreased both the minimal inhibitory concentration and minimal bactericidal concentration values of the antibiotic, with no synergistic effect.
Heavily Labeled Signal Probe for Electrogenerated Chemiluminescence Peptide‐Based Biosensing of Matrix Metalloproteinase 2
Authors: Zhang Danyang , Qian Manping , Yang Xiaolin , Zhang Chengxiao , Qi Honglan 
Published: August 2024

AbstractThe field of electrogenerated chemiluminescence (ECL) biosensing has witnessed remarkable growth, emphasizing the need for precise detection of biomarkers. The synthesis approach of peptide‐based signal probe with high recognition ability and high ECL efficiency is a significant issue in the ECL biosensing. Here, a heavily labeled signal probe was synthesized for ECL peptide‐based biosensing tactic by using a new aldehyde bearing cyclometalated Ir(III) complex ([Ir(bt)2(bpy‐CHO)PF6 (bt=2‐phenylbenzothiazole, bpy‐CHO=4’‐methyl‐[2,2’‐bipyridine]‐4‐carbaldehyde, denoted as Ir1) as ECL signal reagent and streptavidin (SA) as carrier protein. One ECL peptide‐based biosensing method was exemplified for the detection of matrix metalloproteinase 2 (MMP‐2) by using Ir1 labeled SA (SA−Ir1) as heavily labeled signal probe and biotinylated peptide as molecular recognition substrate. MMP‐2 was sensitively detected in the range from 5 to 100 ng/mL with a detection limit of 1.5 ng/mL. Importantly, two detection modes differing in the order of cleavage recognition by MMP‐2 and signal transduction with SA−Ir1 were compared for the first time. First cleavage and second signal transduction were proposed to be beneficial to sensitive detection of target, which provides some ideas for biomarker diagnostics in disease screening at an early stage.
A 3D bioprinted antibacterial hydrogel dressing of gelatin/sodium alginate loaded with ciprofloxacin hydrochloride
Authors: Cao Liuyuan , Lu Yueqi , Chen Hezhi , Su Ya , Cheng YuneYee , Xu Jie , Sun Huanwei , Song Kedong 
Published: August 2024

AbstractSkin plays a crucial role in human physiological functions, however, it was vulnerable to bacterial infection which delayed wound healing. Nowadays, designing an individual wound dressing with good biocompatibility and sustaining anti‐infection capability for healing of chronic wounds are still challenging. In this study, various concentrations of the ciprofloxacin (CIP) were mixed with gelatine (Gel)/sodium alginate (SA) solution to prepare Gel/SA/CIP (GAC) bioinks, following the fabrication of GAC scaffold by an extrusion 3D bioprinting technology. The results showed that the GAC bioinks had good printability and the printed GAC scaffolds double‐crosslinked by EDC/NHS and CaCl2 had rich porous structure with appropriate pore size, which were conducive to drug release and cell growth. It demonstrated that the CIP could be rapidly released by 70% in 5 min, which endowed the GAC composite scaffolds with an excellent antibacterial ability. Especially, the antibacterial activities of GAC7.5 against Escherichia coli and Staphylococcus aureus within 24 h were even close to 100%, and the inhibition zones were still maintained 14.78 ± 0.40 mm and 14.78 ± 0.40 mm, respectively, after 24 h. Meanwhile, GAC7.5 also demonstrated impressive biocompatibility which can promote the growth and migration of L929 and accelerate wound healing. Overall, the GAC7.5 3D bioprinting scaffold could be used as a potential skin dressing for susceptible wounds with excellent antibacterial activity and good biocompatibility to meet urgent clinical needs.
A closed 3D printed microfluidic device for automated growth and differentiation of cerebral organoids from single‐cell suspension
Authors: Kandra Mario , Vanova Tereza , Jongen Vincent A. , Pospíšil Jakub , Novák Josef , Chochola Václav , Buryška Tomáš , Prokop Zbyněk , Hodný Zdeněk , Hampl Ales , Bohaciakova Dasa , Jaros Josef 
Published: August 2024

AbstractThe development of 3D organoids has provided a valuable tool for studying human tissue and organ development in vitro. Cerebral organoids, in particular, offer a unique platform for investigating neural diseases. However, current methods for generating cerebral organoids suffer from limitations such as labor‐intensive protocols and high heterogeneity among organoids. To address these challenges, we present a microfluidic device designed to automate and streamline the formation and differentiation of cerebral organoids. The device utilizes microwells with two different shapes to promote the formation of a single aggregate per well and incorporates continuous medium flow for optimal nutrient exchange. In silico simulations supported the effectiveness of the microfluidic chip in replicating cellular microenvironments. Our results demonstrate that the microfluidic chip enables uniform growth of cerebral organoids, significantly reducing the hands‐on time required for maintenance. Importantly, the performance of the microfluidic system is comparable to the standard 96‐well plate format even when using half the amount of culture medium, and the resulting organoids exhibit substantially developed neuroepithelial buds and cortical structures. This study highlights the potential of custom‐designed microfluidic technology in improving the efficiency of cerebral organoid culture.
Dialysis rolled scaffold bioreactor allows extended production of monoclonal antibody with reduced media use
Authors: Wu Tongyao , Norouzi Mohsen , Park Kidong 
Published: August 2024

AbstractRapidly expanding biopharmaceutical market demands more cost‐effective platforms to produce protein therapeutics. To this end, novel approaches, such as perfusion culture or concentrated fed‐batch, have been explored for higher yields and lower manufacturing costs. Although these new approaches produced promising results, but their wide‐spread use in the industry is still limited. In this study, a dialysis rolled scaffold bioreactor was presented for long‐term production of monoclonal antibodies with reduced media consumption. Media dialysis can selectively remove cellular bio‐wastes without losing cells or produced recombinant proteins. The dialysis process was streamlined to significantly improve its efficiency. Then, extended culture of recombinant CHO cells for 41 days was successfully demonstrated with consistent production rate and minimal media consumption. The unique configuration of the developed bioreactor allows efficient dialysis for media management, as well as rapid media exchange to harvest produced recombinant proteins before they degrade. Taken together, it was envisioned that the developed bioreactor will enable cost‐effective and long‐term large‐scale culture of various cells for biopharmaceutical production.
F‐actin microfilaments affect the LIPUS‐promoted osteogenic differentiation of BMSCs through TRPM7
Authors: Yao Huan , Tang Li , Wang Dong , Pang Hua , Yang Ke 
Published: August 2024

AbstractThe differentiation of bone marrow mesenchymal stem cells (BMSCs) toward osteogenesis can be induced by low‐intensity pulsed ultrasound (LIPUS). However, the molecular mechanisms responsible for LIPUS stimulation are unclear. The possible molecular mechanisms by which LIPUS promotes osteogenic differentiation of BMSCs were investigated in this study. The quantification of alkaline phosphatase (ALP) activity, Alizarin Red S staining, ALP staining, and the establishment of a calvarial defect model were used to evaluate osteogenic effects. Immunofluorescence was performed to observe the expression of microfilaments and transient receptor potential melastatin 7 (TRPM7). The levels of F‐actin/G‐actin and osteogenesis‐related proteins under LIPUS alone or LIPUS combined with cytoskeleton interfering drugs (Cytochalasin D [CytoD] or Jasplakinolide [JA]) were assayed by western blot. Quantitative real‐time reverse transcription polymerase chain reaction was utilized to measure the expression of Trpm7 mRNA. Moreover, adenoviral Trpm7 knockdown was verified using western blot. The results demonstrated that LIPUS promoted bone formation in vivo. Under osteogenic induction in vitro, the osteogenesis of BMSCs induced by LIPUS was accompanied by the depolymerization and rearrangement of microfilaments and increased levels of TRPM7. By perturbing intracellular actin dynamics, CytoD enhanced the pro‐osteogenicity of LIPUS and increased TRPM7 level, while JA inhibited the pro‐osteogenicity of LIPUS and reduced TRPM7 level. Additionally, the knockdown of Trpm7 suppressed the osteogenic promotion of BMSCs induced by LIPUS. The transient depolymerization and rearrangement of the cytoskeleton microfilaments mediated by LIPUS can affect TRPM7 expression and subsequently promote the osteogenesis of BMSCs. This study provides further direction for exploring the molecular mechanism of LIPUS, as a mechanical stress, in facilitating the osteogenic differentiation of BMSCs.
Bicarbonate concentration influences carbon utilization rates and biochemical profiles of freshwater and marine microalgae
Authors: Kusi Philip Asare , McGee Donal , Tabraiz Shamas , Ahmed Asma 
Published: August 2024

AbstractSelecting the optimal microalgal strain for carbon capture and biomass production is crucial for ensuring the commercial viability of microalgae‐based biorefinery processes. This study aimed to evaluate the impact of varying bicarbonate concentrations on the growth rates, inorganic carbon (IC) utilization, and biochemical composition of three freshwater and two marine microalgal species. Parachlorella kessleri, Vischeria cf. stellata, and Porphyridium purpureum achieved the highest carbon removal efficiency (>85%) and biomass production at 6 g L−1 sodium bicarbonate (NaHCO3), while Phaeodactylum tricornutum showed optimal performance at 1 g L−1 NaHCO3. The growth and carbon removal rate of Scenedesmus quadricauda increased with increasing NaHCO3 concentrations, although its highest carbon removal efficiency (∼70%) was lower than the other species. Varying NaHCO3 levels significantly impacted the biochemical composition of P. kessleri, S. quadricauda, and P. purpureum but did not affect the composition of the remaining species. The fatty acid profiles of the microalgae were dominated by C16 and C18 fatty acids, with P. purpureum and P. tricornutum yielding relatively high polyunsaturated fatty acid content ranging between 14% and 30%. Furthermore, bicarbonate concentration had a species‐specific effect on the fatty acid and chlorophyll‐a content. This study demonstrates the potential of bicarbonate as an effective IC source for microalgal cultivation, highlighting its ability to select microalgal species for various applications based on their carbon capture efficiency and biochemical composition.
Anti‐Diabetic and Insulinotropic Effects of p‐Anisic Acid in High‐Fat Diet and Streptozotocin Induced Type‐2 Diabetic Rats
Authors: Vora Vishal , Tirgar Pravin , Raval Keval 
Published: August 2024

AbstractPurpose: To evaluate the antidiabetic effect of p‐anisic acid (p‐AA) against type 2 diabetes mellitus (T2DM). Methods: Thirty‐six male rats were utilized for the study. The animals were provided with the high‐fat diet (HFD) and T2DM was induced through 35 mg/kg streptozotocin (STZ). Subsequently, the animals were allocated to 6 groups and subjected to the treatments for 4 weeks followed by a 2‐week observation. Three treatment groups were administered with p‐AA (25 mg/kg, 50 mg/kg or 100 mg/kg). Glibenclamide (3 mg/kg) was provided to the standard control group. The normal control and disease control groups were supplied with 0.1 % carboxymethyl cellulose (CMC). Subsequently, the effects of treatment on body weight (BW), blood glucose level (BGL), glycosylated haemoglobin (HbA1c), insulin, and lipid parameters were measured and the pancreas was isolated for histopathology. Results: A statistically significant (P‐value<0.001) increase in BW was observed in the p‐AA (50 mg/kg and 100 mg/kg) groups. The BGL decreased significantly (P‐value<0.001) in p‐AA groups. p‐AA significantly (P‐value<0.001) regulated the levels of HbA1c and serum insulin and lipid parameters. A notable improvement in the morphology of the pancreas was observed. Conclusion: Treatment with p‐AA exhibited significant antidiabetic effects against STZ‐HFD‐induced T2DM.
Discovery of Bioactive Terpenes Derived from a Polar Fungus
Authors: Ning Yaodong , Qu Yingxin , Fu Yeqin , Zhang Shi , Xu Yao , Jiao Binghua , Lu Xiaoling 
Published: August 2024

AbstractPolar fungi play a vital role as prolific sources of unique chemical structures and diverse bioactive compounds. Eutypella sp. D‐1 is a fungus isolated from the Arctic, and six compounds were extracted from the fermentation broth. Their structures are elucidated from HRESIMS, NMR spectroscopy, and ECD calculations. Compounds 1–5 are newly discovered compounds, with compound 1 possessing a rare peroxide‐bridge structure. Compounds 1–4 are categorized as pimarane‐type diterpenes, while compounds 5 and 6 belong to the eudesmanolide sesquiterpenes. Compound 4 demonstrates anti‐inflammatory activity by inhibiting lipopolysaccharide‐induced nitric oxide release in RAW264.7 cells. Compounds 4 and 5 show antibacterial activity against Escherichia coli and Staphylococcus aureus.
Three New Steroids from the Roots of Marsdenia tenacissima
Authors: Du Ting‐Ting , Fan Ming‐Hui , Liu Jin‐Ling , Mu Dan‐Dan , Yang Ming‐Xia , Wang Yong‐li , Xu Hong‐Tao , Chou Gui‐Xin 
Published: August 2024

AbstractThree undescribed pregnane steroids, 12β‐O‐4‐hydroxybenzoyl tenacigenin D (1), 12β‐O‐4‐hydroxybenzoyl tenacigenin A (2), and 11α‐nicotinoyl‐17β‐marsdenin (3), along with two known analogues (4 and 5), were isolated from the roots of Marsdenia tenacissima. Their structures were elucidated using one‐ and two‐dimensional NMR, high‐resolution electron ionization‐mass spectrometry, single‐crystal X‐ray diffraction data, and experimental and density‐functional‐theory‐calculated electronic circular dichroism measurements. All isolated compounds were evaluated for their cytotoxic activities against human lung cancer cells (A549), ovarian carcinoma cells (SKOV‐3), gastric cancer cells (MGC 803) and breast cancer cells (MCF‐7). Notably, 3 exhibited significant cytotoxic activity against both A549 (median inhibitory concentration (IC50)=16.79 μM) and SKOV‐3 (IC50=12.30 μM) cells while exhibiting moderate cytotoxicity on MGC803 and MCF‐7 cells.
Levilactobacillus brevis IBARAKI‐TS3 Isolated From Pickles Promotes Production of Interleukin‐10 via Toll‐Like Receptor 2 in Human M2 Macrophages
Authors: Tobita Keisuke , Iwasa Satoru 
Published: August 2024

ABSTRACTM2 macrophages play an important role in food allergy. Several studies have reported that lactic acid bacteria isolated from pickles exert antiallergic effects. We investigated the effects of several strains of lactic acid bacteria on the immune function of M2 macrophages. M2 macrophages differentiated from THP‐1 cell line by interleukin‐4 (IL‐4) and IL‐13 strongly expressed CD163, CD206, and HMOX1 mRNA. Levilactobacillus brevis IBARAKI‐TS3 (IBARAKI‐TS3) isolated from pickles was identified as a lactic acid bacterium that enhances the expressions of IL‐10 and EBI3 mRNA in M2 macrophages. IBARAKI‐TS3 induced the expression of genes involved in Toll‐like receptor (TLR) signaling, such as IRAK, mitogen‐activated protein kinases (MAPKs), and NF‐κB mRNA. IBARAKI‐TS3–induced IL‐10 production was suppressed by anti‐TLR2–neutralizing antibodies. Furthermore, the IBARAKI‐TS3–induced increase in IL‐10 levels was significantly reduced in TLR2–knockdown M2 macrophages compared to M2 macrophages. These results suggest that IBARAKI‐TS3 promotes of IL‐10 production via TLR2 in M2 macrophages.