bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of surgery and mitoxantrone chemotherapy in a dog with disseminated lymphangiosarcoma
Authors: Sicotte Véronique , Benamou Jérôme , Fifle Lyanne C. , Planté Jérôme , Lacoste Hugues I. , Joly Hugo , Stern Adam W. 
Published: December 2012

Abstract
Case Description—A 5-year-old sexually intact male Giant Schnauzer was evaluated because of difficulty breathing and left pelvic limb swelling. Eighteen months previously, the patient had had intermittent left pelvic limb swelling, but the owner declined further testing at that time.
Clinical Findings—Physical examination revealed severe pitting edema of the left pelvic limb and prepuce and muffled heart sounds on thoracic auscultation. Results of thoracic radiography and thoracocentesis were consistent with chylothorax, and CT imaging of the thorax and abdomen revealed a mass involving the whole left sublumbar area.
Treatment and Outcome—In an attempt to treat the chylothorax, pleural omentalization and pericardectomy were performed. Histologic evaluation of several biopsy specimens harvested in the abdominal and thoracic cavities revealed disseminated lymphangiosarcoma. The patient recovered well from surgery, and mitoxantrone chemotherapy was administered. As of 10 months after surgery, the dog was clinically normal except for mild pelvic limb edema.
Clinical Relevance—The combination of clinical signs, multiple imaging features, surgical findings, and histologic examination findings enabled the final diagnosis of lymphangiosarcoma. Clinical management that included medical and surgical treatments and chemotherapy resulted in improved quality of life and extended survival time in a dog with metastatic lymphangiosarcoma.
From macro to lab-scale: Changes in bacterial community led to deterioration of EBPR in lab reactor
Authors: Muszyński Adam , Łebkowska Maria , Tabernacka Agnieszka , Miłobędzka Aleksandra 
Published: December 2012

AbstractA laboratory scale sequencing batch reactor (SBR), fed with synthetic wastewater containing a mixture of organic compounds, was operated for nearly six months. Despite maintaining the same operational conditions, a deterioration of enhanced biological phosphorus removal (EBPR) occurred after 40 days of SBR operation. The Prel/Cupt ratio decreased from 0.28 to 0.06 P-mol C-mol−1, and C requirements increased from 11 to 32 mg C h−1 g−1 of mixed liquor suspended solids. A FISH analysis showed that the percentage of Accumulibacter in an overall community of polyphosphate accumulating organisms (PAOs) and glycogen accumulating organisms (GAOs) dropped from 93% to 13%. An increase in abundance of Gammaproteobacteria (from 2.6% to 22%) and Alphaproteobacteria (from 1.8% to 10%) was observed. The number of Competibacter increased from 0.5% to nearly 9%. Clusters 1 and 2 of Defluviicoccus-related GAOs, not detected before deterioration, constituted 35% and 27% of Alphaproteobacteria, respectively. We concluded that lab-scale experiments should not be extended implicitly to full-scale EBPR systems because some bacterial groups are detected mainlyin lab-scale reactors. Well-defined, lab-scale operational conditions reduce the number of ecological niches available to bacteria.
DREAM regulates insulin promoter activity through newly identified DRE element
Authors: Daneva Teodora , Pashova Shina , Emilova Radoslava , Padeshki Plamen , Gagov Hristo , Georgiev Volodia 
Published: December 2012

AbstractDownstream regulatory element antagonist modulator (DREAM) protein is a 31 kDa Ca2+-regulated transcriptional repressor. It functions as a silencer of the gene transcription. In low intracellular free Ca2+ concentration DREAM tightly binds to the downstream regulatory element (DRE) of gene promoter and impedes the transcription. In higher Ca2+ concentrations DREAM binds Ca2+ and disconnects from DRE of the gene promoter enabling transcription. We report that DREAM is expressed in different human tissues including the pancreas, where it is located in the islets of Langerhans. Location of DREAM in RIN-F5 cells in cultures is restricted to the nucleus and membranes and changes after increased Ca2+-levels. The proteins dissociate from dimmers to monomers and translocate out of the nucleus. The expression of DREAM in β-cells in the islets of Langerhans regulates the promoter activity of the insulin gene by directly interacting with the sequence located between +52 bp and +81 bp downstream of the transcriptional start site of the promoter. Our results provide evidence for the existence of DRE sequence in the insulin gene promoter. It is suggested that DREAM is a repressor of insulin gene transcription, whose effect is mediated by direct binding to DRE sequence.
Sulphur distribution in the products of waste tire pyrolysis
Authors: Susa Dalibor , Haydary Juma 
Published: December 2012

AbstractThe aim of the presented work was to investigate the distribution of sulphur in tire pyrolysis products as well as the influence of process parameters (temperature and residence time) on sulphur distribution due to environmental concerns. Among modern methods used for waste tire recycling, pyrolysis is one of the most reasonable alternatives meeting current environmental standards. However, waste tire sulphur content can be a potential drawback for pyrolysis products utilisation as fuels. Sulphur is present in tires in different concentrations, depending on the type and age of the tires. Typical sulphur content in tires is about 1.6 mass %. In this paper, the distribution of sulphur in tire pyrolysis products was investigated. Tire pyrolysis yields three different products: liquid, gaseous, and solid residue composed mostly of carbon black (chars). Temperature and residence time are the two most important parameters affecting the yield and composition of the volatile fraction and they are therefore expected to affect the sulphur content in residues. Pyrolysis experiments were carried out in a laboratory pyrolysis reaction unit in the temperature range of 650°C to 750°C at different residence times: 88.6 s, 80.2 s, and 73.9 s. Liquid and solid products were analysed by elemental analysis and the distribution of total sulphur in tire pyrolysis products was calculated.
Long-term variations of the seasonal snow cover in Nordland, Norway: the influence of the North Atlantic Oscillation
Authors: Theakstone Wilfred H. 
Published: December 2012

AbstractTemporal and spatial variations of the seasonal snow cover at 40 sites in Nordland county, Norway, since the last decade of the 19th century are examined. Nordland lies across the Arctic Circle. Annual maximum snow depths there have varied, reflecting the interaction of synoptic conditions, temperature and terrain. North/south and coastal/inland differences are evident, but common temporal trends are identified. Maximum snow depths are strongly related to the winter North Atlantic Oscillation index. Early in the 20th century, the index was positive and the associated stormy conditions resulted in a deep, prolonged snow cover. As the index declined in the 1920s, snow depths decreased sharply. Through much of the second half of the 20th century they increased as the index tended to become more positive. The start and duration of the period of continuous snow cover is influenced by the autumn NAO index. A decrease of duration around 1990 was particularly evident at low-lying stations and those in northern Nordland. The NAO has varied considerably over the past 120 years. Because of its influence, forecasting future trends of snow depth and snow-cover duration is not a simple task.
Association of Age with Thyroid Hormone Status and Ischaemic Heart Disease (IHD)
Authors: Lovely Nasreen Sultana , Begum Rokeya , Akhter Qazi Shamima , Akhter Salma , Mili Nilufar Yasmin , Ashraf-uz-zaman Md 
Published: December 2012

Background: Aging is associated with increased prevalence of thyroid function abnormalities including hypothyroidism. A meta-analysis showed an increased prevalence and incidence of cardiovascular mortality only in a relatively younger population. Objective: To compare the thyroid function status in IHD patients of different age groups. Methods: This cross sectional study was carried out on 31 IHD subjects aged 35-59 years (Group B1) and 19 IHD subjects aged 60-85 years (Group B2) in the Department of Physiology, Dhaka Medical College, Dhaka from July 2009 to June 2010. For comparison 24 healthy subjects aged 35-59 years (Group A1) and 26 with age 60-85 years (Group A2) were studied. The IHD subjects were selected from coronary care unit of cardiology department and OPD of Dhaka Medical College Hospital, Dhaka. Serum FT3, FT4 and TSH of all subjects were measured by radioimmunoassay. Statistical analysis was done by unpaired Students t test. Results: The mean ± SD of FT3 and FT4 were significantly lower and TSH was significantly higher in Group B1 IHD patients than that of Group A1 healthy subjects, but no difference was found between Group A2 and Group B2 and between Group B1 and Group B2.Conclusion: Thyroid hormone levels are significantly lower in younger IHD population (age 35-59 years) than the age-matched normal controls. DOI: http://dx.doi.org/10.3329/jemc.v2i2.12842 J Enam Med Col 2012; 2(2): 80-84
A Case of Childhood Ovarian Teratoma
Authors: Bhuiyan Helal Uddin , Afsana Faria , Yasmin Sharmin , Begum Khodeja 
Published: December 2012

Germ cell tumors are malignant (cancerous) or nonmalignant (benign, noncancerous) tumors that are comprised mostly of germ cells, the cells that develop in the embryo and become the cells that make up the reproductive system in male and female. Most ovarian tumors are of germ cell origin. A 5-year-old female child was taken to the Paediatrics department at Tawau General Hospital (TGH), Sabah, Malaysia on 10.4.2005 with gradual distension of abdomen which had been noticed by parents for the last 2 months. As bedside ultrasonography (USG) showed mixed echogenic mass in the lower abdomen, CT scan of abdomen was requested for further delineation. CT scan of abdomen done on 18.4.2005 showed a very large (about 12 × 9 cm) mixed density mass with homogenously enhancing well defined borders in the abdomen extending from the pelvic cavity to the upper abdomen causing significant pressure effect downwards towards urinary bladder and contrast material filled guts outwards and backwards mostly occupying the anterior part of abdominal cavity. The tumor was removed surgically and diagnosed as ovarian teratoma histopathologically. DOI: http://dx.doi.org/10.3329/jemc.v2i2.12846 J Enam Med Col 2012; 2(2): 94-96
Microsatellite loci for tucumã of Amazonas (Astrocaryum aculeatum) and amplification in other Arecaceae
Authors: Ramos Santiago L. Ferreyra , de Macêdo Jeferson L. Vasconcelos , Lopes Maria T. Gomes , Batista Jacqueline S. , Formiga Kyara M. , da Silva Perla Pimentel , Saulo‐Machado Antonio C. , Veasey Elizabeth Ann 
Published: December 2012

• Premise of the study: Microsatellite loci were developed for tucumã of Amazonas (Astrocaryum aculeatum), and cross‐species amplification was performed in six other Arecaceae, to investigate genetic diversity and population structure and to provide support for natural populations management.• Methods and Results: Fourteen microsatellite loci were isolated from a microsatellite‐enriched genomic library and used to characterize two wild populations of tucumã of Amazonas (Manaus and Manicoré cities). The investigated loci displayed high polymorphism for both A. aculeatum populations, with a mean observed heterozygosity of 0.498. Amplification rates ranging from 50% to 93% were found for four Astrocaryum species and two additional species of Arecaceae.• Conclusions: The information derived from the microsatellite markers developed here provides significant gains in conserved allelic richness and supports the implementation of several molecular breeding strategies for the Amazonian tucumã.
Une mission de sauvetage : Exhibitions. L'invention du sauvage au musée du quai Branly
Authors: Arndt Lotte 
Published: December 2012

Résumé Présentée en 2012 par le musée du quai Branly, l’exposition Exhibitions. L’invention du sauvage a été visitée par des milliers de spectateurs et spectatrices et récompensée par le prix de la presse française pour les arts et la culture. Cette exposition ambitieuse proposait de montrer comment la distinction entre « nous » et « les autres » a été mise en scène à travers les siècles. Cette motivation se trouve prise au piège de plusieurs failles conceptuelles et finit par reproduire ce qu’elle aspirait à déconstruire : une rétroprojection anhistorique de concepts, l’accaparement de la parole par la voix omnisciente des commissaires et la surimposition de représentations raciales figées.
Crise de l'offre foncière économique en Algérie : du blocage à l'investissement informel. Le cas de la ville d'Annaba
Authors: Boulbir Laala , Boukhemis Kaddour , Zeghiche Anissa 
Published: December 2012

Avec la libéralisation économique, l’État algérien s’assigne des missions régaliennes et managériales en vue de garantir et attirer les investissements étrangers pour créer des richesses et offrir de l’emploi. Dans cette perspective, l’accès au foncier a été placé au centre de dispositifs procéduraux pour parer à d’éventuels spéculateurs et ne pas brader le patrimoine public de l’État. Après une décennie (1990) d’improvisations, la décennie 2000 marque une volonté de prise en charge sérieuse du dossier foncier. Un état des lieux des ZI (Zones Industrielles) et ZAC (Zones d’Activités Commerciales) a été dressé en vue de rationaliser l’utilisation du potentiel foncier public existant et de le mobiliser pour la promotion de l’investissement privé. L’objet de ce travail est l’analyse du système de pilotage de l’offre foncière publique instauré pour promouvoir l’investissement privé en prenant pour étude de cas, Annaba, la 4 e ville d’Algérie.
Des dispositifs de jugement pour et par les consommateurs ?
Authors: Rodet Diane 
Published: December 2012

Résumé Présentant les propriétés des singularités, les biens issus du commerce équitable, de l’agriculture biologique et les AMAP, offrent un terrain inédit pour étudier la participation des consommateurs aux dispositifs de jugement. Plusieurs réseaux de ces secteurs mettent en effet en place des « systèmes participatifs de garantie » impliquant l’ensemble des parties prenantes de la production dans le processus d’évaluation et de garantie. Ils participent ainsi à une démarche plus globale de résistance face au risque perçu de désingularisation qu’entraînerait la certification par tiers.
Gαi2 Is the Essential Gαi Protein in Immune Complex–Induced Lung Disease
Authors: Wiege Kristina , Ali Syed R. , Gewecke Britta , Novakovic Ana , Konrad Franziska M. , Pexa Katja , Beer-Hammer Sandra , Reutershan Jörg , Piekorz Roland P. , Schmidt Reinhold E. , Nürnberg Bernd , Gessner J. Engelbert 
Published: December 2012

Abstract
Heterotrimeric G proteins of the Gαi family have been implicated in signaling pathways regulating cell migration in immune diseases. The Gαi-protein–coupled C5a receptor is a critical regulator of IgG FcR function in experimental models of immune complex (IC)–induced inflammation. By using mice deficient for Gαi2 or Gαi3, we show that Gαi2 is necessary for neutrophil influx in skin and lung Arthus reactions and agonist-induced neutrophilia in the peritoneum, whereas Gαi3 plays a less critical but variable role. Detailed analyses of the pulmonary IC-induced inflammatory response revealed several shared functions of Gαi2 and Gαi3, including mediating C5a anaphylatoxin receptor–induced activation of macrophages, involvement in alveolar production of chemokines, transition of neutrophils from bone marrow into blood, and modulation of CD11b and CD62L expression that account for neutrophil adhesion to endothelial cells. Interestingly, C5a-stimulated endothelial polymorphonuclear neutrophil transmigration, but not chemotaxis, is enhanced versus reduced in the absence of neutrophil Gαi3 or Gαi2, respectively, and knockdown of endothelial Gαi2 caused decreased transmigration of wild-type neutrophils. These data demonstrate that Gαi2 and Gαi3 contribute to inflammation by redundant, overlapping, and Gαi-isoform–specific mechanisms, with Gαi2 exhibiting unique functions in both neutrophils and endothelial cells that appear essential for polymorphonuclear neutrophil recruitment in IC disease.
Nuclear Translocation of MEK1 Triggers a Complex T Cell Response through the Corepressor Silencing Mediator of Retinoid and Thyroid Hormone Receptor
Authors: Guo Lei , Chen Chaoyu , Liang Qiaoling , Karim Mohammad Zunayet , Gorska Magdalena M. , Alam Rafeul 
Published: December 2012

Abstract
MEK1 phosphorylates ERK1/2 and regulates T cell generation, differentiation, and function. MEK1 has recently been shown to translocate to the nucleus. Its nuclear function is largely unknown. By studying human CD4 T cells, we demonstrate that a low level of MEK1 is present in the nucleus of CD4 T cells under basal conditions. T cell activation further increases the nuclear translocation of MEK1. MEK1 interacts with the nuclear receptor corepressor silencing mediator of retinoid and thyroid hormone receptor (SMRT). MEK1 reduces the nuclear level of SMRT in an activation-dependent manner. MEK1 is recruited to the promoter of c-Fos upon TCR stimulation. Conversely, SMRT is bound to the c-Fos promoter under basal conditions and is removed upon TCR stimulation. We examined the role of SMRT in regulation of T cell function. Small interfering RNA-mediated knockdown of SMRT results in a biphasic effect on cytokine production. The production of the cytokines IL-2, IL-4, IL-10, and IFN-γ increases in the early phase (8 h) and then decreases in the late phase (48 h). The late-phase decrease is associated with inhibition of T cell proliferation. The late-phase inhibition of T cell activation is, in part, mediated by IL-10 that is produced in the early phase and, in part, by β-catenin signaling. Thus, we have identified a novel nuclear function of MEK1. MEK1 triggers a complex pattern of early T cell activation, followed by a late inhibition through its interaction with SMRT. This biphasic dual effect most likely reflects a homeostatic regulation of T cell function by MEK1.
Ofatumumab Is More Efficient than Rituximab in Lysing B Chronic Lymphocytic Leukemia Cells in Whole Blood and in Combination with Chemotherapy
Authors: Bologna Luca , Gotti Elisa , Da Roit Fabio , Intermesoli Tamara , Rambaldi Alessandro , Introna Martino , Golay Josée 
Published: December 2012

Abstract
Ofatumumab (OFA) is a human anti-CD20 Ab approved for treatment of fludarabine-refractory B chronic lymphocytic leukemia (B-CLL). The efficacy of different immunotherapeutic strategies is best investigated in conditions that are as physiologic as possible. We have therefore compared the activity OFA and rituximab (RTX), alone or in combination with chemotherapeutic agents in unmanipulated whole blood assays, using flow cytometry. OFA (10–100 μg/ml) lysed B-CLL targets in whole blood more efficiently and with faster kinetics than RTX, with a mean 56% lysis at 24 h compared with 16%. This activity of OFA was fully complement dependent, as shown by &gt;99% inhibition by anti-C5 Ab eculizumab and a lack of NK cell activation in whole blood. OFA-mediated NK cell activation was blocked by complement. OFA-mediated lysis could be increased an additional 15% by blocking CD55 and CD59 complement inhibitors. Interestingly, OFA-mediated lysis correlated significantly with CD20 expression levels (r2 = 0.79). OFA showed overlapping dose response curves similar to those for RTX in phagocytosis assays using either human macrophages or neutrophils. However, phagocytosis was inhibited in the presence of serum or whole blood. Finally, combined treatment with mafosfamide and fludarabine showed that these therapeutic drugs are synergistic in B-CLL whole blood assays and show superior activity when combined with OFA compared with RTX. These results confirm in B-CLL samples and in physiologic conditions the superior complement mediated cytotoxicity induced by OFA alone compared with RTX, the lack of NK cell activation, and phagocytosis in these conditions and suggest effective chemoimmunotherapy strategies using this new generation anti-CD20 Ab.
Design of Porous Three-Dimensional PDLLA/nano-hap Composite Scaffolds Using Stereolithography
Authors: Ronca Alfredo , Ambrosio Luigi , Grijpma Dirk W. 
Published: December 2012

Purpose The stereolithography process is based on the photopolymerization through a dynamic mask generator of successive layers of photocurable resin, allowing the manufactory of accurate micro objects with high aspect ratio and curved surfaces. In the present work the stereolithography technique is applied to produce nanocomposite bioactive scaffolds from Computer Assisted Design (CAD) files. Methods Porous scaffolds are designed with computer software and built with a composite poly(D,L-lactide)/nano hydroxyapatite based resin. Triply-periodic minimal surfaces are shown to be a more versatile source of biomorphic scaffold designs and scaffolds with Double Gyroid architecture are realized and characterized from morphological, mechanical and biological point of view. Results The structures show excellent reproduction of the design and good mechanical properties. Human marrow mesenchimal cells (hMSC) are seeded onto porous PDLLA composites for 3 weeks and cultured in osteogenic medium. Presence of nano-hap seems to increase the mechanical properties without affecting the morphology of the structures. The composite Double Gyroid scaffolds exhibit good biocompatibility and confirm that nano-hap enhances the scaffold bioactive and os-teoconductive potential. Conclusion The presented technology and materials enable an accurate preparation of tissue engineering composite scaffolds with a large freedom of design, and really complex internal architectures. Results indicate that the scaffolds fulfill the basic requirements of bone tissue engineering scaffold, and have the potential to be applied in orthopedic surgery.
Integrated Bi‐Layered Scaffold for Osteochondral Tissue Engineering
Authors: Galperin Anna , Floreani Rachael A. , Florczyk Stephen J. , Bryers James D. , Zhang Miqin , Ratner Buddy D. 
Published: December 2012

AbstractOsteochondral tissue engineering poses the challenge of combining both cartilage and bone tissue engineering fundamentals. In this study, a sphere‐templating technique was applied to fabricate an integrated bi‐layered scaffold based on degradable poly(hydroxyethyl methacrylate) hydrogel. One layer of the integrated scaffold was designed with a single defined, monodispersed pore size of 38 μm and pore surfaces coated with hydroxyapatite particles to promote regrowth of subchondral bone while the second layer had 200 μm pores with surfaces decorated with hyaluronan for articular cartilage regeneration. Mechanical properties of the construct as well as cyto‐compatibility of the scaffold and its degradation products were elucidated. To examine the potential of the biphasic scaffold for regeneration of osteochondral tissue the designated cartilage and bone layers of the integrated bi‐layered scaffold were seeded with chondrocytes differentiated from human mesenchymal stem cells and primary human mesenchymal stem cells, respectively. Both types of cells were co‐cultured within the scaffold in standard medium without soluble growth/differentiation factors over four weeks. The ability of the integrated bi‐layered scaffold to support simultaneous matrix deposition and adequate cell growth of two distinct cell lineages in each layer during four weeks of co‐culture in vitro in the absence of soluble growth factors was demonstrated.