bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhodium‐Catalyzed Annulative Coupling of 3‐Phenylthiophenes with Alkynes Involving Double C‐H Bond Cleavages
Authors: Iitsuka Tomonori , Hirano Koji , Satoh Tetsuya , Miura Masahiro 
Published: November 2013

AbstractDouble CH bond activation took place efficiently upon treatment of 3‐phenylthiophenes with alkynes in the presence of a rhodium catalyst and a copper salt oxidant to form the corresponding naphthothiophene derivatives. Dehydrogenative coupling with alkenes was also found to occur on the phenyl moiety rather than the thiophene ring. These reactions provide straightforward synthetic methods for π‐conjugated molecules involving a thiophene unit from readily available, simple building blocks.
Differential localization of NT‐3 and TrpM5 in glomeruli of the olfactory bulb of mice
Authors: Rolen S.H. , Salcedo E. , Restrepo D. , Finger T.E. 
Published: November 2013

ABSTRACTOlfactory sensory neurons that express transient receptor potential channel M5 (TrpM5) or neurotrophin‐3 (NT‐3) project to defined clusters of glomeruli situated ventrally in the main olfactory bulb. Using genetically labeled mice, we investigated whether expression of NT‐3‐driven βgal and TrpM5‐driven GFP marked overlapping sets of glomeruli and whether expression of these markers was coordinated. Our results indicate that these markers largely characterize independent sets of olfactory sensory neuron axons and glomeruli. Further, in glomeruli in which both TrpM5‐GFP and NT‐3‐βgal labeled axons occur, they are expressed independently. The nature of staining for these two markers also differs within glomeruli. Within each labeled TrpM5‐positive glomerulus, the level of TrpM5‐GFP expression was similar throughout the glomerular neuropil. In contrast, NT‐3‐driven βgal expression levels are heterogeneous even within heavily labeled glomeruli. In addition, a population of very small TrpM5‐GFP positive glomeruli is apparent while no similar populations of NT‐3‐βgal glomeruli are evident. Taken together, these data suggest that TrpM5 and NT‐3 characterize two largely independent receptor populations both conveying odorant information to the ventral olfactory bulb. J. Comp. Neurol. 522:1929–1940, 2014. © 2013 Wiley Periodicals, Inc.
Subcortical connections of area V4 in the macaque
Authors: Gattass Ricardo , Galkin Thelma W. , Desimone Robert , Ungerleider Leslie G. 
Published: November 2013

ABSTRACTArea V4 has numerous, topographically organized connections with multiple cortical areas, some of which are important for spatially organized visual processing, and others which seem important for spatial attention. Although the topographic organization of V4's connections with other cortical areas has been established, the detailed topography of its connections with subcortical areas is unclear. We therefore injected retrograde and anterograde tracers in different topographical regions of V4 in nine macaques to determine the organization of its subcortical connections. The injection sites included representations ranging from the fovea to far peripheral eccentricities in both the upper and lower visual fields. The topographically organized connections of V4 included bidirectional connections with four subdivisions of the pulvinar, two subdivisions of the claustrum, and the interlaminar portions of the lateral geniculate nucleus, and efferent projections to the superficial and intermediate layers of the superior colliculus, the thalamic reticular nucleus, and the caudate nucleus. All of these structures have a possible role in spatial attention. The nontopographic, or converging, connections included bidirectional connections with the lateral nucleus of the amygdala, afferent inputs from the dorsal raphe, median raphe, locus coeruleus, ventral tegmentum and nucleus basalis of Meynert, and efferent projections to the putamen. Any role of these structures in attention may be less spatially specific. J. Comp. Neurol. 522:1941–1965, 2014. © 2013 Wiley Periodicals, Inc.
Phthalocyanine‐Based Organometallic Nanocages: Properties and Gas Storage
Authors: Zhu Guizhi , Li Yawei , Lü Kun , Sun Qiang 
Published: November 2013

AbstractPhthalocyanine (Pc) molecules are well‐known flexible structural units for 1D nanotubes and 2D nanosheets. First‐principles calculations combined with grand canonical Monte Carlo simulations are used to obtain the geometries, electronic structures, optical properties, and hydrogen‐storage capacities of nanocages consisting of six Pc molecules with six Mg or Ca atoms. The primitive Pc cage has Th symmetry with twofold degeneracy in the highest occupied molecular orbital (HOMO), and threefold degeneracy in the lowest unoccupied molecular orbital (LUMO); the corresponding HOMO–LUMO gap is found to be 0.97 eV. The MgPc and CaPc cages have Oh symmetry with a HOMO–LUMO gap of 1.24 and 1.13 eV, respectively. Optical absorption spectra suggest that the Pc‐based cages can absorb infrared light, which is different from the visible‐light absorption in Pc molecules. We further show that the excess uptake of hydrogen on MgPc and CaPc cages at 298 K and 100 bar (1 bar=0.1 MPa) is about 3.49 and 4.74 wt %, respectively. The present study provides new insight into Pc‐based nanostructures with potential applications.
Immobilization of LaW10 onto Ionic‐Liquid‐Modified Mesoporous Silica: Deep Desulfurization with Zero‐Order Reaction Kinetics
Authors: Chen Yang , Song Yu‐Fei 
Published: November 2013

AbstractThe polyoxometalate (POM) cluster Na7H2LaW10O36⋅32 H2O (denoted LaW10) is immobilized onto dihydroimidazolium‐based ionic‐liquid (IL)‐modified mesoporous silica, resulting in the formation of a new catalyst: LaW10/IL‐SiO2. Compared with other reported extractive catalytic oxidative desulfurization (ECODS) systems, LaW10/IL‐SiO2 has the following advantages: 1) the heterogeneous catalyst LaW10/IL‐SiO2 achieves deep desulfurization of dibenzothiophene (DBT), benzothiophene (BT), and 4,6‐dimethyldibenzothiophene (4,6‐DMDBT) in 25, 30, and 25 min, respectively, under mild conditions; 2) deep desulfurization of DBT follows zero‐order reaction kinetics; 3) there is no need to add ionic liquid each time the catalyst is reused; 4) the catalyst can be separated easily by filtration and reused at least ten times without a decrease in catalytic efficiency. As such, LaW10/IL‐SiO2 shows great potential for practical applications.
Age‐related changes in stem cell dynamics, neurogenesis, apoptosis, and gliosis in the adult brain: A novel teleost fish model of negligible senescence
Authors: Traniello Ian M. , Sîrbulescu Ruxandra F. , Ilieş Iulian , Zupanc Günther K.H. 
Published: November 2013

ABSTRACTAdult neurogenesis, the generation of new neurons in the adult central nervous system, is a reported feature of all examined vertebrate species. However, a dramatic decline in the rates of cell proliferation and neuronal differentiation occurs in mammals, typically starting near the onset of sexual maturation. In the present study, we examined possible age‐related changes associated with adult neurogenesis in the brain of brown ghost knifefish (Apteronotus leptorhynchus), a teleost fish distinguished by its enormous neurogenic potential. Contrary to the well‐established alterations in the mammalian brain during aging, in the brain of this teleostean species we could not find evidence for any significant age‐related decline in the absolute levels of stem/progenitor cell proliferation, neuronal and glial differentiation, or long‐term survival of newly generated cells. Moreover, there was no indication that the amount of glial fibrillary acidic protein or the number of apoptotic cells in the brain was altered significantly over the course of adult life. We hypothesize that this first demonstration of negligible cellular senescence in the vertebrate brain is related to the continued growth of this species and to the lack of reproductive senescence during adulthood. The establishment of the adult brain of this species as a novel model of negligible senescence provides new opportunities for the advancement of our understanding of the biology of aging and the fundamental mechanisms that underlie senescence in the brain. © 2013 Wiley Periodicals, Inc. Develop Neurobiol 74: 514–530, 2014
Modeling human epilepsy by TALEN targeting of mouse sodium channel Scn8a
Authors: Jones Julie M. , Meisler Miriam H. 
Published: November 2013

SummaryTo evaluate the efficiency of TALEN technology for introducing mutations into the mouse genome we targeted Scn8a, a member of a multigene family with nine closely related paralogs. Our goal was to generate a model of early onset epileptic encephalopathy by introduction of the Scn8a missense mutation p.Asn1768Asp. We used a pair of TALENs that were highly active in transfected cells. The targeting template for homologous recombination contained a 4 kb genomic fragment. Microinjection of TALENs with the targeting construct into the pronucleus of 350 fertilized mouse eggs generated 67 live‐born potential founders, of which 5 were heterozygous for the pathogenic mutation, a yield of 7% correctly targeted mice. Twenty‐four mice carried one or two Scn8a indels, including 12 frameshift mutations and the novel amino acid deletion p.Asn1759del. Nine off‐site mutations in the paralogs sodium channel genes Scn5a and Scn4a were identified. The data demonstrate the feasibility and efficiency of targeting members of multigene families using TALENs. The Scn8atm1768DMm mouse model will be useful for investigation of the pathogenesis and therapy of early onset seizure disorders. genesis 52:141–148. © 2013 The Authors genesis Published by Wiley Periodicals, Inc.
Towards consistent hybrid overset mesh methods for rotorcraft CFD
Authors: Jarkowski M. , Woodgate M.A. , Barakos G.N. , Rokicki J. 
Published: November 2013

SUMMARYThe overset mesh method chimera is popular within the rotorcraft research community, because the use of multiple, non‐matching grids make the CFD simulations of bodies in relative motion much simpler. Consequently, the relative motion between the helicopter blades and fuselage can be accurately accounted for. In this paper, the method for treating overset grids within CFD codes is presented. It is compatible with multi‐block, structured‐grid solvers. The proposed method is based on hierarchy of overset, non‐matching grids, whose cells are automatically identified as computational or non‐computational and localised with respect to all grids they overlap with. The efficiency of the method relies on the hierarchical, multi‐step approach, for the overset mesh localisation and the use of a tree search. Because of the high efficiency of the algorithm, the search for overlapping cells can be carried out on‐the‐fly, during time‐marching of the unsteady, implicit CFD solver. In addition, the algorithm is suitable for parallel execution. The method has been demonstrated for several flows, ranging from simple aerofoils to rotor‐body interaction. The paper presents and demonstrates the method and shows that it has a low CPU overhead. It also highlights the limitations of the method and suggests remedies for improvement. Copyright © 2013 John Wiley & Sons, Ltd.
Transoral robotic surgery of the parapharyngeal space: A case series and systematic review
Authors: Chan Jason Y. K. , Tsang Raymond K. , Eisele David W. , Richmon Jeremy D. 
Published: November 2013

ABSTRACTBackgroundThe purpose of this study was to evaluate the current use of transoral robotic surgery (TORS) in the treatment of parapharyngeal space (PPS) neoplasms through a case series and systematic analysis.MethodsA case series review of 4 patients was combined with a PubMed, Web of Science, and Scopus search that identified 82 reports. Fifty‐three articles remained after screening for duplicates, finally, 8 reports with adequate patient data were included. Statistical analyses and graphical representations were performed with Microsoft Excel (Redmond, WA) and GraphPad Prism software (La Jolla, CA).ResultsForty‐four patients had TORS resection of PPS neoplasms. Overall, mean length of stay was 3.0 days with mean time to oral diet of 1.0 day. There were no recurrences but there was a mean follow‐up time of only 18.5 months. Twenty‐nine of these neoplasms (65.9%) were pleomorphic adenomas of which 7 (24%) had unintended capsule violation or tumor fragmentation during surgery and 2 patients had pharyngeal dehiscence that was managed conservatively. There were no neurovascular complications.ConclusionTORS is a viable approach to resection of neoplasms of the PPS with minimal surgical morbidity. However, further long‐term evaluation, especially for pleomorphic adenomas, is needed to define patient selection and the role of TORS for PPS salivary gland neoplasms. © 2013 Wiley Periodicals, Inc. Head Neck 37: 293‐298, 2015
Regional risk assessment of the Puyallup River Watershed and the evaluation of low impact development in meeting management goals
Authors: Hines Eleanor E , Landis Wayne G 
Published: November 2013

Abstract
The Relative Risk Model (RRM) is a tool used to calculate and assess the likelihood of effects to endpoints when multiple stressors occur in complex ecological systems. In this study, a Bayesian network was used to calculate relative risk and estimate uncertainty (BN-RRM) in the Puyallup River Watershed. First, we calculated the risk of prespawn mortality of coho salmon. Second, we evaluated the effect of low impact development (LID) as a means to reduce risk. Prespawner mortality in coho salmon within the Puyallup watershed was the endpoint selected for this study. A conceptual model showing causal pathways between stressors and endpoints was created to show where linkages exist. A relative risk gradient was found throughout the watershed. The lowest risk was found in risk regions with the least urban development, and the greatest risk of prespawner mortality was found in the highly urbanized risk regions with the largest amounts of impervious surface. LID did reduce risk but only when implemented at high intensities within the urban watersheds. The structure of the BN-RRM also provides a framework for water quality- and quantity-related endpoints within this and other watersheds. The framework is also useful for evaluating different strategies for remediation or restoration activities. The adaptability of using BNs for a relative risk assessment provides opportunities for the model to be adapted for other watersheds in the Puget Sound and Salish Sea region. Integr Environ Assess Manag 2014;10:269–278. © 2013 SETAC
Key Points
The Bayesian network relative risk model (BN-RRM) estimated risk of prespawn mortality to Coho salmon in the Puyallup River watershed in a spatially explicit manner. The pattern of risk was that a greater risk existed as the watershed became more urban as it transitions through National Park, agriculture, suburbs, manufacturing and eventually to the Port of Tacoma. The effect of low impact development (LID) was successfully introduced into the model to examine the changes in risk. To successfully alter the pattern of risk, a substantial amount of area would have to be transferred to LID, especially in the high-risk regions of the watershed. The adaptability of the BN-RRM allows for both an evaluation of risk and an examination of how management alternatives alter the risk. The fundamental approach should be applicable to other sites in Puget Sound and to other scenarios.
INTEGRATED STEPWISE APPROACH FOR OPTIMAL WATER ALLOCATION IN IRRIGATION CANALS
Authors: Kanooni Amin , Monem Mohammad Javad 
Published: November 2013

ABSTRACTOptimal water management in irrigation networks should be an integrated approach which includes both canal and field systems. Integrated optimal water management of irrigation networks requires consideration of both optimal allocation of water among different crops at field level, and optimal water delivery scheduling at the canal level, which is often neglected. In this study, an integrated stepwise approach has been presented for considering both farm‐ and canal‐level optimization problems. Three modules are proposed for the optimization model. In the first module, crop–water production functions for different crops are produced, and optimal seasonal allocation of available water among several crops is determined using an LP algorithm. The objective function of this module is to maximize total net benefit of all crops. The second module takes the optimal allocated water to each crop from the first module, and determines its optimal distribution through the growing season for each crop, such that the total yield at the end of the season is maximized. Taking into account the output of the second module, and cultivated area of each crop, the water requirement volume for each secondary canal is computed. The third module determines optimal water delivery scheduling among outlets of the secondary canal using a genetic algorithm (GA). The objective of the third module is to minimize canal flow, and to maximize delivery adequacy and beneficial water delivery duration. The decision variables are delivery discharge to each outlet, the number of outlets grouped in a block which receive water sequentially, and the sequence of water delivery to the outlets in each block. The proposed approach was applied on the K canal of the Moghan Irrigation Network in the north‐west of Iran. The optimal water delivery scheduling of a secondary canal, based on the optimal water allocation among several crops, and intra‐seasonal irrigation scheduling are presented, which shows the utility of the integrated approach. Copyright © 2013 John Wiley & Sons, Ltd.
In vitro analysis of the antibacterial effect of nanohydroxyapatite–ZnO composites
Authors: Grenho Liliana , Jorge Monteiro Fernando , Pia Ferraz Maria 
Published: November 2013

AbstractHydroxyapatite (HA) is a biocompatible and bioactive synthetic material for biomedical applications as it binds to bone and enhances bone tissue formation. Particularly, nanophased HA can mimic the dimensions of constituent components of natural tissues; can modulate enhanced osteoblast adhesion and resorption with long‐term functionality of tissue engineered implants. However, HA does not inhibit bacteria from adhering onto its surface, and this has implications in the bone healing process required for patient recovery, since infection can lead to the implant failure. In the present work a composite that combines the favorable biological characteristics of nanohydroxyapatite (nanoHA) and, simultaneously, possesses antimicrobial activity as expressed by ZnO was synthesized. To determine whether the size of ZnO particles was playing an important role in inhibiting bacterial growth, ZnO particle of different sizes (from the microscale down to the nanoscale) and concentration were incorporated into nanoHA and tested. The composite samples were characterized by SEM, FT‐IR, XRD, XPS and zeta potential. The antibacterial activity of the composites was investigated, as well as the biofilm formation, using both Gram‐positive (Staphylococcus aureus) and Gram‐negative (Escherichia coli) microorganisms. The characterization revealed that ZnO particles were dispersed homogeneously within the nanoHA matrix. The composites antibacterial activity increased with decreasing ZnO particle size and increasing concentration. Biofilm formation tests revealed that the nanoHA‐ZnO composites exhibit a strong effect against the common pathogens S. aureus and E. coli. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3726–3733, 2014.
MicroRNA‐590 Promotes Cervical Cancer Cell Growth and Invasion by Targeting CHL1
Authors: Chu Yanxia , Ouyang Yunwei , Wang Fei , Zheng Ai , Bai Liping , Han Ling , Chen Yali , Wang Hui 
Published: November 2013

ABSTRACTMicroRNAs (miRNAs) may function as oncogenes or tumor suppressors. Here, we identified that miR‐590‐5p was up‐regulated in human cervical cancer. Over‐expression of miR‐590‐5p promoted cervical cancer cell growth, cell cycle and invasion via Growth curve, Colony formation, FACS and Transwell assays in HeLa and C33A cell lines. Subsequently, CHL1 was identified as a potential miR‐590‐5p target by bioinformatics analysis. Moreover, we showed that CHL1 was negatively regulated by miR‐590‐5p at the posttranscriptional level, via a specific target site within the 3′UTR by luciferase reporter assay. Furthermore, the mRNA and protein levels of CHL1 in cervical cancer cells were downregulated by miR‐590‐5p. And we identified the cell phenotype altered by miR‐590‐5p can be rescued by over‐expression of CHL1. Therefore, our findings suggest that miR‐590‐5p acts as an oncogene by targeting the CHL1 gene and promotes cervical cancer proliferation. The findings of this study contribute to current understanding of the functions of miR‐590‐5p in cervical cancer. J. Cell. Biochem. 115: 847–853, 2014. © 2013 Wiley Periodicals, Inc.
Cytoplasmic PML: From Molecular Regulation to Biological Functions
Authors: Jin Guoxiang , Gao Yuan , Lin Hui‐Kuan 
Published: November 2013

ABSTRACTThe tumor suppressor promyelocytic leukemia protein (PML) is predominantly localized in the nucleus, where it is essential for the formation and stabilization of the PML nuclear bodies (PML‐NBs). PML‐NBs are involved in the regulation of numerous cellular functions, such as tumorigenesis, DNA damage and antiviral responses. Despite its nuclear localization, a small portion of PML has been found in the cytoplasm. A number of studies recently demonstrated that the cytoplasmic PML (cPML) has diverse functions in many cellular processes including tumorigenesis, metabolism, antiviral responses, cell cycle regulation, and laminopothies. In this prospective, we will summarize the current viewpoints on the regulation and biological significance of cPML and discuss the important questions that still need to be further answered. J. Cell. Biochem. 115: 812–818, 2014. © 2013 Wiley Periodicals, Inc.
Environmental changes in Baffin Bay during the Holocene based on the physical and magnetic properties of sediment cores
Authors: ST‐ONGE MARIE‐PIER , ST‐ONGE GUILLAUME 
Published: November 2013

ABSTRACTThe physical and magnetic properties of four long sediment cores (HU2008‐029‐034PC, ‐038PC, ‐042PC and ‐070PC) sampled in Northern (Smith Sound and Jones Sound) and Eastern (Disko Bugt) Baffin Bay were analysed to reconstruct the Holocene environmental changes in Baffin Bay. Radiocarbon dating of each core revealed sedimentation rates of up to 136 cm ka−1. Except for specific intervals, magnetic properties and ratios reveal that the magnetic remanence is mostly carried by magnetite and that changes in magnetic grain size and concentration are indicative of environmental variations associated with ice‐rafted debris, sea‐ice, meltwater pulses or terrigenous inputs. These variations indicate that all four cores, which cover a period from 12 360 cal a BP to the present, have sedimentary facies that correspond to the major climatic changes of the Holocene: deglaciation and the climatic optimum. In addition, two cores (HU2008‐029‐038PC and ‐070PC) present the signal of two climatic events with a local influence during the Neoglacial period.