bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping and Candidate Gene Analysis of an All-Stage Stem Rust Resistance Gene in Durum Wheat Landrace PI 94701
Authors: Li Hongyu , Li Kairong , Li Hongna , Yang Chen , Perera Geetha , Wang Guiping , Lyu Shikai , Hua Lei , Rehman Shams ur , Zhang Yazhou , Ayliffe Michael , Yu Haitao , Chen Shisheng 
Published: August 2024

Puccinia graminis f. sp. tritici (Pgt), the causal agent of wheat stem rust, poses a significant threat to global wheat production. Genetic resistance offers a cost-effective and sustainable solution. The durum wheat landrace PI 94701 was previously hypothesized to carry two stem rust resistance (Sr) genes, but their chromosomal locations were unknown. In this study, we mapped and characterized an all-stage Sr gene in PI 94701, temporarily designated as SrPI94701. In seedling tests, SrPI94701 was effective against all six Pgt races tested. Using a large segregating population, we mapped SrPI94701 on chromosome arm 5BL within a 0.17-cM region flanked by markers pku69124 and pku69228, corresponding to 1.04 and 2.15 Mb genomic regions in the Svevo and Chinese Spring reference genomes. Within the candidate region, eight genes exhibited differential expression between the Pgt-inoculated resistant and susceptible plants. Among them, two nucleotide-binding leucine-rich repeat (NLR) genes, TraesCS5B03G1334700 and TraesCS5B03G1335100, showed high polymorphism between the parental lines and were upregulated in Pgt-inoculated resistant plants. However, the flanking and completely linked markers developed in this study could not accurately predict the presence of SrPI94701 in a survey of 104 wheat accessions. SrPI94701 is a promising resource for enhancing stem rust resistance in wheat breeding programs.
Implementing a Compliance Monitoring Process to Promote Chlorhexidine Gluconate Bathing and Hand Hygiene: An Initiative to Decrease Central Line–Associated Bloodstream Infections
Authors: Davis Tanisha , Wittmann Susanne , Prairie Beth A. , Dugan Nancy , Reiser Patricia , Goclano Leah , Dziobak Rose 
Published: August 2024

Background Chlorhexidine gluconate (CHG) bathing has proven to reduce central line–associated bloodstream infections (CLABSI) in hospitalized patients. The aim of this study is to evaluate whether the implementation of a compliance monitoring process for CHG bathing and strict hand hygiene as part of the traditional CLABSI prevention bundle will reduce the overall hospital CLABSI standardized infection ratio (SIR). Methods A quasi-experimental study was conducted in intensive care and non-intensive care inpatient hospital units in an urban 361-bed teaching hospital in Western Pennsylvania. The pre-intervention period consisted of January–August 2021, the intervention period consisted of September–November 2021, and the post-intervention and sustainability period consisted of December 2021–June 2022. A compliance monitoring process for the initiation of a daily bath using a 4% CHG solution and strict hand hygiene surveillance was implemented as part of the standard CLABSI prevention bundle (which includes standardized insertion checklists and processes, daily necessity assessment, dressing change and integrity standards, five moments hand hygiene standards, CHG disk [Biopatch], end caps [Curos], and care of tubing). Outcomes A statistically significant increase in hand hygiene (p=<0.001) and in CHG bathing compliance (p=0.014) helped reduce the overall hospital CLABSI SIR from 1.45 to 0.82, standing for an overall 43.4% decrease. Cost reduction was statistically significant (p=0.011) and was an estimated $1.4 million dollars in savings for the hospital. Next Steps Incorporate the compliance monitoring component for CHG bathing and hand hygiene as part of the best strategy for CLABSI bundle prevention within the healthcare system.
Challenges to quality in contemporary, hybrid general practice a multi-site longitudinal case study
Authors: Payne Rebecca , Dakin Francesca , MacIver Ellen , Swann Nadia , Pring Tabitha , Clarke Aileen , Kalin Asli , Moore Lucy , Ladds Emma , Wherton Joseph , Rybczynska-Bunt Sarah , Husain Laiba , Hemmings Nina , Wieringa Sietse , Greenhalgh Trisha 
Published: August 2024

Background
Since 2022, general practice has shifted from responding to the acute challenges of COVID-19 to restoring full services using a hybrid of remote, digital, and in-person care.

Aim
To examine how quality domains are addressed in contemporary UK general practice.

Design and setting
Multi-site, mostly qualitative longitudinal case study, placed in UK national policy context.

Method
Data were collected from longitudinal ethnographic case studies of 12 general practices (2021–2023), multi-stakeholder workshops, stakeholder interviews, patient surveys, official reports, and publicly accessible patient experience data. Data were coded thematically and analysed using multiple theories of quality.

Results
Quality efforts in UK general practice occur in the context of cumulative impacts of financial austerity, loss of resilience, increasingly complex patterns of illness and need, a diverse and fragmented workforce, material and digital infrastructure that is unfit for purpose, and physically distant and asynchronous ways of working. Providing the human elements of traditional general practice (such as relationship-based care, compassion, and support) is difficult and sometimes even impossible. Systems designed to increase efficiency have introduced new forms of inefficiency and have compromised other quality domains such as accessibility, patient-centredness, and equity. Long-term condition management varies in quality. Measures to mitigate digital exclusion (such as digital navigators) are welcome but do not compensate for extremes of structural disadvantage. Many staff are stressed and demoralised.

Conclusion
Contemporary hybrid general practice features changes (digitalisation, physical distancing, extension of roles, and protocolisation) that have had the unintended effect of dehumanising, compromising, and fragmenting care. Policymakers and practices should urgently address the risks to patients and the traditional core values of general practice should be urgently addressed.
Tratamento água de abastecimento humano a partir de carvão granulado de açaí ativado quimicamente
Authors: Vieira José Sebastião Cidreira , Ano João Darlon Alves do , Melo Gabriela Ribeiro , Pereira Agustinho Rodrigues , Reis Alexandre da Costa , Carvalho Francisco Wanderson Pinto de , Carvalho Edson Mauro Viana de , Rodrigues Makson Rangel de Melo 
Published: August 2024

O carvão ativado é um dos materiais mais utilizados no processo de adsorção, principalmente no tratamento de água para abastecimento humano. Neste contexto, o objetivo deste trabalho foi produzir um carvão granulado ativado quimicamente a partir de caroços de açaí. A carbonização do precursor foi realizada a 700 ºC durante 30 minutos, o ácido fosfórico (H3PO4) foi empregado como agente de ativação. No decurso do tratamento de água de poço artesiano desativado o carvão apresentou elevado potencial adsortivo eliminando e/ou redimindo os inconvenientes físico e químicos aumentando significativamente a potabilidade da água destinada ao abastecimento humano e contribuindo para a melhoria da qualidade de vida dos
Análise criminológica do encarceramento feminino pela prática do crime de tráfico de drogas
Authors: Araújo Mayara Stéffany da Silva , Silva Ivan Luiz Rufino da 
Published: August 2024

O presente artigo pretende, assim como o tema proposto remete, a analisar o crime de tráfico de drogas praticado por mulheres, verificando de forma crítica os fatores condicionantes da criminalidade feminina. A pergunta de pesquisa que guia esta pesquisa é: quais fatores que condicionam o ingresso feminino na prática do crime de tráfico de drogas? A partir da indagação apresentada, fora analisado o fenômeno da criminalidade feminina no tráfico de drogas a partir de um viés crítico, buscando compreender, através dos estudos da criminologia feminista marginal e das teorias que justificam o ingresso na atividade delituosa, as principais motivações que influenciam a entrada da mulher no crime em comento. Superado o problema de pesquisa, buscou-se alternativas aptas a produção de efeitos positivos no tocante a redução do encarceramento feminino pelo tráfico de drogas, destacando que medidas desencarceradoras apresentam-se como possível solução a problemática, vez que a atual política probicionista de drogas é ineficaz. Desta forma, para alcançar os resultados obtidos nesta pesquisa, foram utilizadas as seguintes técnicas e procedimentos metodológicos: i) revisão bibliográfica em obras de ciências sociais, direito penal e criminologia, quanto das autoras que se debruçam sobre as relações das mulheres com o crime a partir de um referencial teórico feminista; ii) análise de dados quantitativos e qualitativos extraídos de órgãos oficiais, atentando-se a limitação que eles apresentam, vez que existe uma grande restrição de informações.
Pembuatan Pulp dan Kertas dengan Memanfaatkan Ampas Sereh Wangi (Cymbopogon Nardus L)
Authors: Marlina Ari , Widiastuti Endang , Suryadi Joko 
Published: August 2024

Ampas atau limbah sereh wangi merupakan limbah dari penyulingan minyak sereh wangi yang banyak mengandung serat selulosa, hemiselulosa dan lignin. Kandungan selulosa tersebut, berpotensi sebagai sumber bahan baku pembuatan pulp dan kertas. Dalam penelitian ini, ampas tersebut dibuat pulp dan kertas melalui proses semi-mekanik. Mula mula dibuat pulp dengan penambahan larutan pemasak NaOH yang berbeda-beda konsentrasinya, dengan perbandingan 1:8. Proses pemasakan berlangsung pada suhu 100oC, selama 90 menit dengan perbandingan padat-cair 1:8 (b/v). Pulp terbaik diperoleh dengan kadar selulosa 68,7% dan kadar lignin 10,10% dalam larutan NaOH 20%, dimasak pada suhu 100oC selama 90 menit. Selanjutnya pulp diproses untuk pembuatan kertas, dengan penambahan tapioka1,5%; 2%; 2,5%; 3%; 3,5%. Berdasar hasil uji karakterisasi terhadap hasil kertas, nilai tertinggi dari ketahanan tarik dan ketahanan sobek diperoleh sebesar 1,04 kN/m dan 781,8 N dengan gramatur 91,3 g/m2 pada penambahan tapioka 3%.
Pengaruh Kawasan Wisata Ke’te’ Kesu’ Terhadap Lingkungan Sekitarnya Di Kabupaten Toraja Utara
Authors: Lembang Lembang , Salim Agus , Rasyidi Emil Salim 
Published: August 2024

Abstract. This study aims to analyze how the influence of the Ke'te' Kesu' tourist area on the surrounding environment in North Toraja Regency and what are the community empowerment strategies in supporting the development of the Ke'te' Kesu' area. This study uses a quantitative approach method with the process of collecting data through observation, documentation and questionnaire surveys where the sampling method uses purposive sampling. The data obtained is then analyzed using the chi-square analysis test and SWOT analysis. From the results of the chi-square test analysis that has been carried out, it can be concluded that the areas that have the most influence on the environment of the Ke'te' Kesu' tourist area are the variables of attractiveness, facilities, safety and comfort. Then, from the results of the SWOT analysis, a community empowerment strategy was obtained, namely increasing the social and economic aspects of the community, involving the community in managing the potential of the ke'te' kesu' area, involving the support of the ke'te' kesu' community to make it a strategic tourist attraction and establishing good communication between the sub-district government and the North Toraja Tourism Office.
Abstrak.Penelitian ini bertujuan untuk menganalisis mengetahui bagaimana pengaruh kawasan wisata Ke’te’ Kesu’ terhadap lingkungan sekitarnya di Kabupaten Toraja Utara dan bagaimana strategi pemberdayaan masyarakat dalam mendukung pengembangan kawasan Ke’te’ Kesu’.Penelitian ini menggunakan metode pendekatan kuantitatif dengan proses pengambilan data melalui kegiatan observasi,dokumentasi dan survei kuesioner dimana penarikan sampelnya menggunakan metode purpose sampling.data yang diperoleh selanjutnya dianalisis dengan menggunakan uji analisis chi-square dan analisis SWOT.Dari hasil analisis uji chi-square yang telah dilakukan dapat disimpulkan bahwa kawasan yang paling berpengaruh terhadap lingkungan kawasan wisata Ke’te’ Kesu’ adalah variabel daya tarik, sarana, keamanan dan kenyamanan. Kemudian dari hasil analisis SWOT diperoleh strategi pemberdayaan masyarakat yaitu meningkatan aspek sosial dan ekonomi masyarakat,Mengikutsertakan masyarakat dalam mengelola Potensi kawasan ke’te’ kesu’,melibatkan dukungan masyarakat ke’te’ kesu’ untuk menjadikan objek wisata yang strategis dan Menjalin komunikasi yang baik antara pemerintah kelurahan dengan dinas pariwisata toraja utara.
Aplikasi Teknologi Crispr/Cas9 Dalam Anti-Aging Medicine
Authors: Siswanto Ferbian Milas , Kartiko Bambang Hadi 
Published: August 2024

ABSTRAK Seiring dengan proses penuaan, kemampuan gen yang mengontrol viabilitas sel mengalami penurunan, sejalan dengan meningkatnya aktivitas gen yang menyebabkan kematiansel dan berujung pada patogenesis penyakit. Dengan memanipulasi aktivasi dan non-aktivasi gen yang terlibat dalam proses penuaan, maka kesehatan akan meningkat dan proses penuaandapat dihambat bahkan dikembalikan. Saat ini telah ditemukan teknologi CRISPR/Cas-9 yangmemungkinkan manipulasi pada tingkat genetik. CRISPR sebenarnya merupakan bagian prosesfisiologis pada bakteri, yang merupakan salah satu sistem imunitas bakteri. Namun saat iniCRISPR telah banyak dibuktikan dan diaplikasikan untuk reprogram DNA pada sel mamaliadan dapat mengembalikan perubahan genetik yang tidak diinginkan.Kata kunci: Penuaan, CRISPR/Cas-9, anti-aging medicine, manipulasi genetik ABSTRACTAs all living thing undergo aging, genes that maintain cellular viability and vitality are downregulated, in line with upregulated expression of genes that promote cells senescence anddisease-related pathogenesis. By manipulating the initiation and inhibition of aging-relatedgene expression, the youthful health may be systemically restored. Recently, a new technologycalled CRISPR is found to be usefull for manipulating gene and rapidly transform senescentcells to regain youthful function and structure. CRISPR is originally developed in nature by bacteria as a way to destroy the DNA of viruses which part of their immune system. A naturalversion of CRISPR has been adapted by scientists to enable the reprogramming of cellular DNAto rid cells of unfavorable genetic changes. Keyword: Aging, CRISPR/Cas-9, anti-aging medicine, genetic manipulation
Potensi Ekstrak Etanol Daun Kelor (Moringa oleifera) Terhadap Penurunan Kadar Histamin Pada Ikan Lemuru (Sardinella longiceps)
Authors: Valent Febryna Aurora , Oka Adi Parwata I Made , Susanah Rita Wiwik 
Published: August 2024

ABSTRAK Tanaman kelor merupakan suku Moringaceae yang telah menjadi obyek pada banyakpenelitian karena mengandung senyawa aktif seperti flavonoid, saponin, tanin, dan senyawafenolik lain yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahuipotensi ekstrak etanol daun kelor (Moringa oleifera) terhadap penurunan kadar histamin padaikan lemuru (Sardinella longiceps). Penelitian ini juga bertujuan untuk mengetahui konsentrasi minimum ekstrak etanol daun kelor dan penentuan kadar total flavonoid yang terdapat di dalamekstak etanol daun kelor. Variasi konsentrasi ekstrak etanol yang digunakan adalah 0%, 7%,8%, 9%, 10%, dan 15% dengan lama perendaman selama 0, 30, 60, dan 90 menit. Kadarhistamin diuji dengan alat Biofish dan hasilnya dianalisis dengan menggunakan Two WayANOVA untuk mengetahui perbedaan yang berarti sebagai respon terhadap perlakuan. Hasilpenelitian menunjukkan bahwa variasi konsentrasi ekstrak dan lama perendaman memilikiperbedaan yang nyata (P<0,05) dan konsentrasi minimum ekstrak yang dapat menurunkan kadar histamin adalah 8%. Kadar total flavonoid pada ekstrak etanol daun kelor yang digunakanadalah 3177,11 mg/100 g QE.Kata kunci: Sardinella longiceps, Moringa oleifera, histamin, flavonoidABSTRACT Moringa plants are Moringaceae tribes that have been the object of many studiesbecause they contain active compounds such as flavonoids, saponins, tannins, and other potentially antibacterial phenolic compounds. The aim of this research is to know the potency ofmoringa leaf ethanol extract (Moringa oleifera) to decrease histamine levels in lemuru fish(Sardinella longiceps). This study also aims to determine the minimum concentration ofmoringa leaf ethanol extract and the determination of total flavonoid content c ontained inethanol extract of moringa leaf. Variations of ethanol extract concentration used were 0%, 7%,8%, 9%, 10%, and 15% with soaking time for 0, 30, 60, and 90 min. Histamine levels weretested with a Biofish tool and the results were analyzed using Two Way ANOVA to determinethe significant differences in response to the treatment. The results showed that v ariation of extract concentration and soaking time had significant difference (P<0,05) and the minimumconcentration of extract which could decrease histamine level was 8%. The total flavonoidcontent of moringa leaf ethanol extract used was 3177.11 mg/100 g QE.Keywords: Sardinella longiceps, Moringa oleifera, histamine, flavonoids
Fitoremediasi Limbah Cair UPT Laboratorium Analitik Universitas Udayana Menggunakan Tanaman Eceng Gondok (Eichhornia crassipes) Ditinjau dari Penurunan Nilai COD dan Kandungan Logam Berat Cu dan Cr
Authors: Welhelmus Djo Yuliana Herman , Suastuti Dwi Adhi , Eka Suprihatin Iryanti , Sulihingtyas Wahyu Dwijani 
Published: August 2024

ABSTRAKUPT Laboratorium Analitik Universitas Udayana menghasilkan limbah khususnya limbah cair yang mengandung bahan kimia berbahaya. Sistem pengolahan limbah yaituFitoremediasi menggunakan eceng gondok (Eichhornia crassipes) bertujuan untuk mengetahui efektivitas penurunan COD (Chemical Oxygen Demand), kandungan logam berat Cu dan Crlimbah cair laboratorium analitik dan mengetahui daya serap eceng gondok terhadap COD danlogam berat Cu dan Cr. Penelitian ini dilakukan dengan membiarkan 840 gram eceng gondoktumbuh dalam 5 liter sampel limbah cair UPT Laboratorium Analitik Universitas Udayanaselama 14 hari. COD dan kandungan logam berat Cu dan Cr dalam limbah diukur setiap hariselama perlakuan. Hasil penelitian menunjukkan terjadinya penurunan COD, kandungan logamberat Cu dan Cr, yang diduga akibat adanya aktivitas biologi yang mengoksidasi senyawa organik maupun anorganik yang terkandung dalam air limbah. Konsentrasi awal COD, Cu danCr sebelum perlakuan adalah 47,04; 0,375; dan 2,58 mg/L dan setelah perlakuan selama 14 harimenjadi 26,34; 0,111; dan 0,72 mg/L. Efektivitas penurunan COD, Cu dan Cr berturut-turut42,36%, 68,73%, dan 42,40%. Daya serap eceng gondok terhadap COD, Cu, dan Cr berturutturut0,1232;0,0016; dan 0,0051 mg/g eceng gondok. Kata kunci: COD, daya serap, eceng gondok, fitoremediasi, limbah laboratorium ABSTRACT The study research processing of liquid waste of the Udayana University AnalyticalLaboratory in way phytoremediation using water hyacinth (Eichhornia crassipes).Phytoremediation using water hyacinth (Eichhornia crassipes) was conducted to reduce theCOD, Cu, and Cr contents of liquid waste of the Udayana University Analytical Laboratory.The study was conducted by allowing 840 grams of water hyacinth to grow in 5 dm of theliquid waste for 14 days. The COD, Cu and Cr concentrations in the liquid waste weremeasured everyday for 14 days, to determine the reduction efectivity and the adsorption capacity of the water hyacinth on those parameters. Results show that all parameters droppedwithin the 14 day treatment from 47.04; 0.375; and 2.58 mg/L to 26.34; 0.111; and 0.72 mg/Lfor COD, [Cu], and [Cr] respectively. The reduction efectivity for the respective parameterswere 42.36%, 68.73%, and 42.4%. The adsorption capacity were 0.1232; 0.0016; and 0.0051mg/g water hyacinth respectively.Keywords: Adsorption,COD, Eceng Gondok, Liquid Waste, Phytoremediation
Struktur Morfologi Bunga Dan Anatomi Serbuk Sari Buah Naga Super Merah (Hylocereus costaricensis)
Authors: Yunita Sari Ni Kadek 
Published: August 2024

ABSTRAK Penelitian ini dilakukan untuk mengetahui struktur morfologi bunga dan anatomi serbuk sari buah naga super merah (Hylocereus costaricensis). Hasil pengamatan menunjukkan strukturmorfologi bunga buah naga super merah dari luar ke dalam terdiri dari kelopak, mahkota bunga,putik dengan kepala putik lebih tinggi dari kepala sari, dan bakal buah. Anatomi serbuk saribunga buah naga daging super merah memiliki kelas ukuran berdasarkan indeks P/E bentuksubspheroidal, apertura tritreme dengan tipe sulkus, dan termasuk kelompok magna.Kata kunci: morfologi,anatomi, serbuk sari,buah naga, sulkus ABSTRACT The purpose of this study is to know the morphological structure of the flowers andanatomy of the pollen of super red dragon fruit (Hylocereus costaricensis). The results showedthe morphological structure of super red dragon fruit flowers from outside to inside consists ofsepala, petala, pistil with stigma more higher than anthera, and ovary. Anatomical pollen ofsuper red dragon f ruits have a size class based on P / E index of the subspheroidal shape, apertura tritreme with sulcus type, and included magna group. Keywords: morphology, anatomy, pollen, dragon fruit, sulcus
Terapi Testosteron Meningkatkan Jumlah Sel Leydig dan Spermatogenesis Mencit (Mus Musculus) yang Mengalami Hiperlipidemia
Authors: Putri Permatasari Anak Agung Ayu , Widhiantara I Gede 
Published: August 2024

ABSTRAKTujuan penelitian ini adalah untuk memperoleh data dan informasi mengenai efek pemberian terapi testosteron untuk meningkatkan jumlah sel Leydig dan sel sel spermatogenikpada mencit yang mengalami hiperlipidemia. Sampel dalam penelitian ini adalah mencit jantandewasa strain Balb-C umur 2 – 3 bulan dengan kisaran berat badan 20-25 gram, sebanyak 24ekor mencit secara random dibagi dua kelompok yaitu 12 ekor mencit kelompok kontrol(hiperlipidemia dan terapi aquadest steril) dan 12 ekor mencit kelompok perlakuan(hiperlipidemia dan terapi testosteron secara intramuscular). Sebelum perlakuan, mencit-mencittersebut dibuat hiperlidemia dengan memberi makanan lemak babi selama 30 hari. Pada prettestdilakukan pemeriksaan jumlah sel Leydig dan sel-sel spermatogenik secara histopatologi kelompok mencit hiperlipidemia sebanyak 6 ekor, dan 6 ekor sisanya merupakan posttestsetelah diberikan perlakuan terapi aquadest pada kelompok kontrol dan testosteron selama 30hari pada kelompok perlakuan. Hasil penelitian menunjukkan adanya penurunan jumlah selLeydig dan sel-sel spermatogenik secara bermakna (p<0,05) pada kelompok hiperlipidemia dansetelah diberi terapi testosteron menunjukkan adanya peningkatan jumlah sel Leydig dan sel-selspermatogenik secara bermakna (p<0,05) apabila dibandingkan dengan kelompokhiperlipidemia. Berdasarkan hasil penelitian ini dapat disimpulkan bahwa terapi testosteron dapat meningkatkan jumlah sel Leydig dan sel-sel spermatogenik pada mencit yang mengalamihiperlipidemia.Kata kunci : Testoseron, Sel Leydig, Spermatogenesis, Mencit, Hiperlipidemia ABSTRACTThis study aims to look at the histopathologic features of corvus muscle cavernosum penis wistar rat (Rattus novergicus) hyperlipidemia seen in terms of thickness as well as apicture of tissue abnormality. Hyperlipidemia is a risk factor for erectile dysfunctionateriosklerosis in men. This research is an experimental research with one group prettest -posttest design. Samples in this study were adult male aged 3-4 months wistar rats with a weightrange 150-200 grams, as many as 10 tails. Prior to treatment, surgery was performed for histopathology of 5-tailed rat samples at pretest. Sample mice were made hyperhidemic byfeeding pig fat for 50 days. Then performed surgery for histopathological preparation of corvuscavernosum muscle in posttest. The histopathological data of the corvus muscle of the wistar ratcavernosum was analyzed descriptively by comparing the pretest condition with posttest. Themean data of corvus cavernosum muscle thickness before and after treatment were analyzedstatistically to see the level of difference in the pretest group with posttest. Kata kunci : Testoseron, Sel Leydig, Spermatogenesis, Mencit, Hiperlipidemia