bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isopsoralen Improves Glucocorticoid-induced Osteoporosis by Regulating Purine Metabolism and Promoting cGMP/PKG Pathway-mediated Osteoblast Differentiation
Authors: Liu Defeng , Ma Lingyun , Zheng Jihui , Zhang Zhenqun , Zhang Nana , Han Zhongqian , Wang Xuejie , Zhao Jianyong , Lv Shuquan , Cui Huantian 
Published: July 2024

Background:
The effects of Isopsoralen (ISO) in promoting osteoblast differentiation and inhibiting
osteoclast formation are well-established, but the mechanism underlying ISO's improvement of Glucocorticoid-
Induced Osteoporosis (GIOP) by regulating metabolism remains unclear.

Methods:
This study aims to elucidate the mechanism of ISO treatment for GIOP through non-targeted
metabolomics based on ISO's efficacy in GIOP. Initially, we established a GIOP female mouse model and assessed
ISO's therapeutic effects using micro-CT detection, biomechanical testing, serum calcium (Ca), and
phosphorus (P) level detection, along with histological analyses using hematoxylin and eosin (HE), Masson,
and tartrate-resistant acidic phosphatase (TRAP) staining. Subsequently, non-targeted metabolomics was employed
to investigate ISO's impact on serum metabolites in GIOP mice. RT-qPCR and Western blot analyses
were conducted to measure the levels of enzymes associated with these metabolites. Building on the
metabolomic results, we explored the effects of ISO on the cyclic Guanosine Monophosphate (cGMP)/Protein
Kinase G (PKG) pathway and its role in mediating osteoblast differentiation.

Results:
Our findings demonstrate that ISO intervention effectively enhances the bone microarchitecture and
strength of GIOP mice. It mitigates pathological damage, such as structural damage in bone trabeculae, reduced
collagen fibers, and increased osteoclasts, while improving serum Ca and P levels in GIOP mice. Non--
targeted metabolomics revealed purine metabolism as a common pathway between the Control and GIOP
groups, as well as between the ISO high-dose (ISOH) group and the GIOP group. ISO intervention upregulated
inosine and adenosine levels, downregulated guanosine monophosphate levels, increased Adenosine Deaminase
(ADA) expression, and decreased cGMP-specific 3',5'-cyclic phosphodiesterase (PDE5) expression. Additionally,
ISO intervention elevated serum cGMP levels, upregulated PKGI and PKGII expression in bone tissues,
as well as the expression of Runt-related transcription factor 2 (Runx2) and Osterix, and increased serum
Alkaline Phosphatase (ALP) activity.

Conclusion:
In summary, ISO was able to enhance the bone microstructure and bone strength of GIOP mice
and improve their Ca, P, and ALP levels, which may be related to ISO's regulation of purine metabolism and
promotion of osteoblast differentiation mediated by the cGMP/PKG pathway. This suggests that ISO is a potential
drug for treating GIOP. However, further research is still needed to explore the specific targets and clinical
applications of ISO.
Effect of Marham-i-Bāsalīqūn on Cervical Erosion (Qurūḥ al-Raḥim): A Randomized Comparative Clinical Study
Authors: Khanum Nadiya , Shameem Ismath , Siddiqui Sumaiya 
Published: July 2024

Background and Objective:
Cervical erosion (Qurūḥ al-Raḥim) is one of the most frequent
gynecological conditions seen in clinical practice. Therefore, the objective of the current
study was to assess the impact of the topical application of Marham-i-Bāsalīqūn in Qurūḥ al-
Raḥim.

Material and Method:
A randomized comparative clinical study was carried out at the Institute
Hospital in Bengaluru. Diagnosed cases (n=40) of cervical erosion were randomly assigned to test
(n=20) and control (n=20) groups. A total of 6 g ointment of Marham-i-Bāsalīqūn (test drug) and
tested Unani formulation (control drug) was applied with an applicator per vaginum over the eroded
area of the cervix daily once at bedtime for 14 days. A single dose of the antibiotic regimen
(azithromycin-1g and cefixime-400 mg) was administered orally in both groups. Subjective parameters
like vaginal discharge, pruritus vulvae, low back ache, lower abdominal pain, and postcoital
bleeding were assessed during the trial. The main outcome measure was an improvement in
the cervical erosion grading score. The inference was executed with appropriate statistical analysis.

Result:
Improvement in the cervical erosion grading score was achieved in 50% and 40% of patients
in the control and test groups, respectively, after 2 weeks of treatment with P<0.0001, which
was considered highly significant. A highly significant improvement in subjective parameters was
observed during the trial with P<0.0001.

Conclusion:
Considering the high prevalence of cervical erosion among women of reproductive
age group, effective treatment and safe strategies are highly recommended. Marham-i-Bāsalīqūn is
cost-effective and has several therapeutic actions that can help to promote the healing of erosion
and relieve the associated symptoms. The clinical trial was registered at the Clinical Trial Registry
of India (CTRI) with registration number CTRI/2022/02/040419.
Global Highly Cited Publication Trends and Research Hotspots in Osteoporosis and Bone Metabolic Cells: A Bibliometric and Visualization Analysis from 2013 to 2023
Authors: Ye Lingshan , Hua Zhen , Ding Xinxin , Wang Jianwei 
Published: July 2024

Background:
Bone metabolic diseases such as osteoporosis are caused by disruption
of the metabolic balance between osteoblasts and osteoclasts. Thousands of papers have been
published on osteoporosis and bone metabolizing cells. The purpose of this study is to draw the
publication trend of highly cited literature in this field through bibliometrics and to explore the
research hotspot analysis.

Objective:
This paper provides a comprehensive analysis of the impact of countries/regions,
research institutions, authors, keywords, relevant journals, and references in the field of osteoporosis
and bone metabolic cells research, with a specific focus on the theme of "Osteoporosis
and bone metabolic cells". Furthermore, utilizing bibliometric methods, the study aims to offer
valuable insights and references for future research endeavors, as well as clinical prevention and
treatment strategies in this domain.

Methods:
The Web of Science (WOS) Core Collection database was examined in order to identify
articles with high citation counts from 2013 to 31 October 2023. The citation counts, authors,
year of publication, source, journal, geographical origin, subject, article type, and level of
evidence were further analyzed using the R bibliometric package. The VOSviewer software was
utilized to visualize word co-occurrence in a total of 251 articles.

Results:
Our search strategy included 251 highly cited articles published between 2013 and
2023 in the field of osteoporosis and bone metabolic cells. The number of publications in this
field remains consistently high, indicating ongoing research interest. Notably, the United States
has made significant achievements and contributions in this area. Xie Hui, Cao Xu, and Goodman,
Stewart are among the main contributors to these advancements. Nature medicine has the
highest journal impact factor of 82.9, highlighting its prominence. The journal of bone and mineral
research ranks first with 1,322 citations. Keyword research topics in this field include osteoclast
differentiation, osteoblast differentiation, and mesenchymal stem cells. Through citation
analysis, we found that 195 articles have been cited more than 100 times, demonstrating their
significance and impact.

Conclusion:
This study analyzed the relationship between osteoporosis and bone metabolic
cells using a bibliometric method. The results of these analyses can help researchers gain a more
direct and scientific understanding of trends in the field. Additionally, it can provide guidance in
identifying hot research directions and offer new ideas for the prevention and treatment of osteoporosis.
Time- and Region-specific Effect of Vortioxetine on Central LPS-induced Transcriptional Regulation of NLRP3 Inflammasome
Authors: Ciani Miriam , Rigillo Giovanna , Benatti Cristina , Pani Luca , Blom Johanna M.C. , Brunello Nicoletta , Tascedda Fabio , Alboni Silvia 
Published: July 2024

Background:
Inflammasome overactivation, multiprotein complexes that trigger inflammatory
responses, plays a critical role in Major Depressive Disorder (MDD) pathogenesis and treatment
responses. Indeed, different antidepressants alleviate depression-related behaviours by specifically
counteracting the NLRP3 inflammasome signalling pathway. The immunomodulatory effects of vortioxetine
(VTX), a multimodal antidepressant with cognitive benefits, were recently revealed to counter
memory impairment induced by a peripheral lipopolysaccharide (LPS) injection 24 hours (h) postchallenge.
The potential link between VTX and NLRP3, along with other inflammasomes, remains
un-explored.

Methods:
The potential link between VTX and NLRP3, along with other inflammasomes, remains unexplored.
Hence, adult C57BL/6J male mice (n = 73) were fed with a standard or VTX-enriched diet
(600 mg/kg of food, 28 days), injected with LPS (830 μg/kg) or saline, and sacrificed 6/24 h post-LPS.
At these time-points, transcriptional effects of LPS and VTX on NLRP3, NLRP1, NLRC4, AIM2 (inflammasomes),
ASC and CASP1 (related subunits) and NEK7 mediator (NLRP3 regulator) were assessed
in dorsal and ventral hippocampal subregions, frontal-prefrontal cortex and hypothalamus,
brain regions serving behavioural-cognitive functions impaired in MDD.

Results:
Varied expression patterns of inflammasomes were revealed, with long-term NLRP3 and
ASC transcriptional changes observed in response to LPS. It was demonstrated that VTX counteracted
the LPS-mediated NLRP3 and ASC upregulation in memory-related brain areas like the dorsal hippocampus
at 24 h time-point, potentially via regulating NEK7 expression. No VTX-mediated transcriptional
effects were observed on other inflammasomes, reinforcing a potentially specific modulation on
the NLRP3 inflammasome signalling pathway.

Conclusion:
Thus, a novel VTX molecular mechanism in modulating the NLRP3 inflammasome in a
time- and area-specific manner in the brain was highlighted, with significant clinical implications in
treating depression and cognitive impairments.
Study to Evaluate the Comparative Efficacy of Medhya Rasayana (Pharmacological) Versus Nonpharmacological Interventions in Management of Gadget Addiction in Children: Protocol for Parallel, Triple-Arm, Randomized Clinical Trial
Authors: Yewale Prasad , Rathi Renu , Mate Swapnali 
Published: July 2024

Background
Gadget addiction is a common behavioral problem among children. It is known to hamper social and academic life as well as adversely affect the lives of children. Ayurveda offers many therapeutic modalities and Ayurvedic medicines that can be used in the management of gadget addiction in children. The purpose of this study is to evaluate and compare the effectiveness of nonpharmacological therapies and the pharmaceutical intervention Medhya Rasayana in treating childhood gadget addiction.

Objective
This study aims to provide a detailed description of the study methodology that will be used to compare the efficacy of nonpharmacological versus pharmaceutical interventions in the treatment of children’s gadget addiction.

Methods
A randomized, parallel, triple-arm interventional study will be conducted on diagnosed participants of gadget addiction with an age group of 6- to 16-year-old children, which will be selected and equally distributed in 2 groups. Group P will be given Medhya Rasayana (pharmacological intervention), group N will be nonpharmacological Ayurveda intervention, and group C (cognitive behavioral therapy) will be an external group. The Study duration is 180 days with assessment at baseline, midpoint, and endpoint Appropriate statistical techniques, such as ANOVA and regression analysis, will be used to examine the data and evaluate the efficacy of the 3 groups’ interventions. We will perform subgroup analysis according to initial addiction severity, gender, and age. Primary outcome measures include a reduction in gadget addiction and changes in the psychosocial well-being of participants. Standardized questionnaires and instruments will be used to collect data.

Results
In December 2023, the randomized controlled study got underway. Since participants may begin at any time, our goal is for everyone to be finished by December 2024.

Conclusions
This research will provide crucial new information about the relative effectiveness of Ayurveda nonpharmacological therapies and Medhya Rasayana in treating children’s gadget addiction. The results will guide evidence-based treatments aimed at reducing the negative impact of excessive gadget use on this susceptible population’s psychosocial development. In the end, the findings are meant to help policy makers and medical professionals create sensible plans to deal with the rising issue of childhood gadget addiction.

International Registered Report Identifier (IRRID)
PRR1-10.2196/51833
Éléments de sémiotique stratégique. La pertinence dans les portefeuilles de marque
Authors: Perusset Alain 
Published: July 2024

L’intérêt des sémioticiens pour le marketing a porté initialement sur les publicités et la communication, puis, à partir des années 1990, sur les enjeux de sens de la consommation et du branding. A cette époque, le marketing s’intéressait déjà aux marques, avec le développement de modèles d’architecture de marque comme ceux de D.A. Aaker ou J.-N. Kapferer. Aujourd’hui, les propositions théoriques de ces deux spécialistes continuent à marquent la pratique du brand management mais elles ne suffisent pas à lever certaines ambiguïtés et tensions inhérentes à l’élaboration de portefeuilles stratégiques de marque. L’objectif du présent article est de proposer une façon pertinente de construire un portefeuille de marque, conformément aux enseignements de la sémiotique structurale (A.J. Greimas) et de la sémantique interprétative (Fr. Rastier). Pour appuyer nos propositions, nous analysons le portefeuille de la marque de céréales Farmer (groupe Migros). Cette démarche conceptuelle a vocation à institutionnaliser une « sémiotique stratégique » offrant un appareil théorique, méthodologique et pratique pour optimiser les décisions stratégiques dans le domaine du brand management.
Helminth Fauna of Bats of the Rostov Region: Review. 2. Cestoda
Authors: Evsyukov A. P. , Potapenko I. O. , Tsygankova M. G. 
Published: July 2024

Introduction. The class Cestoda is divided into two subclasses: the Cestodaria — unsegmented tapeworms, and the Eucestoda — true cestodes. The representatives of the orders Pseudophyllidea and Cyclophyllidae, which are part of the subclass of true cestodes parasitize in carnivorous mammals. At the same time, only representatives of the latter order parasitize in chiropterans. Data on the species composition and distribution of cestodes in the Rostov region have not been published yet. The list of bat species living in the Rostov region have been compiled previously. In this article, the authors study the cestode fauna parasitizing in chiropterans in the region under study. This review aims to analyse the current state of knowledge about the species composition of helminths parasitizing in bats in the Rostov region.Materials and Methods. The research materials have been obtained from the libraries and open access resources: PubMed (pubmed.ncbi.nlm.nih.gov), CyberLeninka (cyberleninka.ru), Google Scholar (scholar.google.com), BHL (www.biodiversitylibrary.org ), JSTOR (www.jstor.org), etc. Some data has been provided by the colleagues. Results. 17 species of cestodes of 4 genera, 1 family and 1 order can be found in 15 species of bats living in the Rostov region. Among them dominate the species of the genus Vampirolepis (11 species).Discussion and Conclusion. The results of the review carried out showed that maximum diversity of cestodes was found in one species of bats — the serotine bat (10 species). The smallest number of cestode species (1 per each) was found in the giant and lesser noctules. None of the cestode species found in bats in the Rostov region were recorded in companion animals or humans. However, some cestode species of the Hymenolepididae family, common in rodents, can infect the humans.
KINERJA SISTEM AGRIBISNIS USAHA PENGGEMUKAN SAPI DI DESA ADIREJO KECAMATAN TERBANGGI BESAR KABUPATEN LAMPUNG TENGAH (STUDI KASUS DI PT INDO PRIMA BEEF)
Authors: Indah Iva Mutiara , Lestari Dyah Aring Hepiana , Sayekti Wuryaningsih Dwi 
Published: July 2024

This study aims to analyze the performance of the agribusiness system of the beef cattle fattening business at PT Indo Prima Beef. The research method used is the case study method, located at PT Indo Prima Beef, Terbanggi Besar District of Central Lampung Regency. Data were collected in November-December 2022. Data were analyzed using qualitative and quantitative descriptive analysis. The results showed that the performance of the production facilities supply subsystem was good, as analyzed by the agribusiness index analysis and six right. However, there are still insufficiencies, such as locations close to settlements, feed is not in the right place, and labor is not in the right type, quality, and quantity. The performance of the cultivation subsystem is suitable for cultivation because it has an R/C ratio value of >1, but currently, the company has not conducted mating with artificial insemination. The performance of the marketing subsystem is good as analyzed by the agribusiness index and marketing mix analysis (4P). Beef cattle marketing channels are large traders (abattoirs), retailers, and consumers. The performance of the supporting service subsystem is good, the services utilized by PT Indo Prima Beef are financial institutions, research institutions, government policies, transportation facilities, communication and information technology facilities, and supply stores for production facilities. Overall, the performance of the agribusiness system at PT Indo Prima Beef has been running well with a weighted agribusiness index of 81.51%. Keywords: Agribusiness index, agribusiness system, beef cattle.
SIKAP, KEPUASAN DAN LOYALITAS KONSUMEN SUSU SEGAR YU-ONE MILK DI KOTA BANDAR LAMPUNG
Authors: Fitri Ayu Aulia , Indriani Yaktiworo , Rufaidah Erlina 
Published: July 2024

This research aims to determine the attitudes, purchasing patterns, satisfaction, and loyalty of fresh milk consumers of Yu-One Milk at Jalan Sultan Agung, Way Halim, Kedaton District of Bandar Lampung City. The research method is a survey. The number of respondents in this research are 60 people, selected by non-probability sampling technique, namely accidental sampling. The research data are analyzed by quantitative descriptively using the Fishbein multi-attribute model, Customer Satisfaction Index (CSI) and the loyalty pyramid. Data collection was carried out in October-November 2022. The results showed that the consumers’ attitude toward multi-attribute of Yu-One Milk fresh milk was in the good category, in which score was 181.72 and the most favored attribute by consumers was friendliness services. Most of Yu-One Milk consumers (25%) bought fresh milk with chocolate flavor variants. The average frequency of purchasing Yu-One Milk fresh milk was twice per month with a purchase expenditure of IDR 51,883.00/month. The average purchases amount of Yu-One Milk fresh milk was 1,377 ml/month with the purpose of purchasing to eliminate hunger/thirst (46.67%). Yu-One Milk fresh milk consumer satisfaction was in the very satisfied category with a CSI value of 83.61. Yu-One Milk consumer loyalty was quite good in the form of an inverted pyramid. The switcher buyer value was 46.67%, the habitual buyer value was 48.83%, the satisfied buyer value was 90.00%, the liking the brand value was 86.67% and the committed buyer value was 83.33%. Keywords: attitude, consumer loyalty, fresh milk, purchase pattern, satisfaction.
PENGARUH SUBTITUSI TEPUNG SORGUM PUTIH DALAM PEMBUATAN VANILLA CUPCAKE TERHADAP KUALITAS FISIK DAN DAYA TERIMA KONSUMEN
Authors: Fairuz Nadhira Alya , S I Gusti Ayu Ngurah , Dahlia Mutiara 
Published: July 2024

This research seeks to analyze the impacts of substituting white sorghum flour in the making of vanilla cupcakes on both their physical quality and consumer acceptance. This research was carried out at the Pastry and Bakery Laboratory of Culinary Education, Faculty of Engineering, State University of Jakarta in July 2022 - January 2024. The method used in this research is the experimental method involving vanilla cupcakes with white sorghum flour substitution of 20 percent, 30 percent, and 40 percent. A consumer acceptability test was conducted on 30 semi-trained panelists evaluating various organoleptic aspects. The statistical analysis, utilizing Analysis of Variance (ANOVA) for the physical quality test, revealed a significant influence of white sorghum flour substitutions (20%, 30%, and 40%) on both cupcakes rising power and stability. After conducting the Friedman Test on several aspects, results showed that there is no statistically significant difference between treatments on these following aspects: 1) the color of crumbs; 2) sorghum aroma; 3) sweetness; and 4) the texture of the top of the cupcake. On the other hand, the test showed that there is a statistically significant difference between treatments on the following aspects: 1) butter aroma; 2) the taste of sorghum; 3) crumbs texture; and 4) the color of the top of the cupcake. Further investigation using Tukey's test is necessary for the aspects with observed differences. Ultimately, the most preferred product was the vanilla cupcakes with a 20 percent sorghum flour substitution.Keywords: consumer acceptability, physical quality, vanilla cupcake, white sorghum flour
KINERJA SISTEM AGRIBISNIS KOPI PADA KOPERASI PRODUSEN SRIKANDI MAJU BERSAMA KECAMATAN ULU BELU KABUPATEN TANGGAMUS
Authors: Misrianti Nunik , Lestari Dyah Aring Hepiana , Adawiyah Rabiatul 
Published: July 2024

The purpose of the research is to investigate the performance of coffee agribusiness system at the cooperative namely Produsen Srikandi Maju Bersama. The method used in this study was a case study method, involving 71 coffee farmers members of the cooperative Produsen Srikandi Maju Bersama, five cooperative managements as processor, and three retailers. The samples were selected by snowball sampling method. The data analysis was carried out using the agribusiness index system. The data were collected from May to - July 2022. The results show that subsystem index for the procurement of production, farming, processing, facilities, and supporting services are in good categories. In the meantime, the marketing subsystem’s index for the coffee is categorized as not good. Overall, coffee agribusiness system at the cooperative Produsen Srikandi Maju Bersama is categorized as good with total score of 11,95 (79,68 percent) from a maximum score of 15,00. keywords: agribusiness system performance, coffee, cooperative