bluuebunny/crossref_metadata_2025_split huggingface dataset:
Partners for the optimal organisation of the healthcare continuum for high users of health and social services: protocol of a developmental evaluation case study design
Authors: Hudon Catherine , Chouinard Maud-Christine , Couture Martine , Brousselle Astrid , Couture Eva Marjorie , Dubois Marie-France , Fortin Martin , Freund Tobias , Loignon Christine , Mireault Jean , Pluye Pierre , Roberge Pasquale , Rodriguez Charo 
Published: December 2014

IntroductionCase management allows us to respond to the complex needs of a vulnerable clientele through a structured approach that promotes enhanced interaction between partners. Syntheses on the subject converge towards a need for a better description of the relationships between programmes and their local context, as well as the characteristics of the clienteles and programmes that contribute to positive impacts. The purpose of this project is thus to describe and evaluate the case management programmes of four health and social services centres in the Saguenay-Lac- Saint-Jean region of Québec, Canada, in order to inform their improvement while creating knowledge on case management that can be useful in other contexts.Methods and analysisThis research relies on a multiple embedded case study design based on a developmental evaluation approach. We will work with the case management programme for high users of hospital services of each centre. Three different units of analysis will be interwoven to obtain an in-depth understanding of each case, that is: (1) health and social services centre and local services network, (2) case management programme and (3) patients who are high users of services. Two strategies for programme evaluation (logic models and implementation analysis) will guide the mixed data collection based on qualitative and quantitative methods. This data collection will rely on: (1) individual interviews and focus groups; (2) participant observation; (3) document analysis; (4) clinical and administrative data and (5) questionnaires. Description and comparison of cases, and integration of qualitative and quantitative data will be used to guide the data analysis.Ethics and disseminationThe study protocol was approved by the Ethics Research Boards of the four health and social services centres (HSSCs) involved. Findings will be disseminated by publications in peer-reviewed journals, conferences, and policy and practice partners in local and national government.
Children who leave the emergency department without being seen: a retrospective case note review
Authors: Sparrow Nicola , Pestell Trudie , Clancy Mike 
Published: December 2014

IntroductionChildren who attend an emergency department and then leave without being seen (LWBS) are a major concern as they are a potentially vulnerable group who may come to harm through failure to provide a timely and accessible service.ObjectivesWe wished to establish the size of this population and importantly what subsequently happened to them over the following 7 days from their initial attendance, and the relevance of the College of Emergency Medicine LWBS Quality Indicator (QI 4) to this group.MethodsA retrospective case note review over 6 months of all paediatric attendances who LWBS.SettingSouthampton Paediatric Emergency Department.ResultsDuring a 6-month study period, 10 795 attended, of which 544 (5%) LWBS. 12.6% (69/544) reattended over the next 7 days, of which 14 were admitted, 7 for <12 h.ConclusionsThe majority who LWBS do so during peak times. Very few paediatric patients who LWBS then reattended required admission for >12 h (7/544, 1.3%). The rate of reattendance of those who LWBS and review of their case notes is potentially more valuable than the LWBS rate alone.
Expanding Epigenomics to Archived FFPE Tissues: An Evaluation of DNA Repair Methodologies
Authors: Siegel Erin M. , Berglund Anders E. , Riggs Bridget M. , Eschrich Steven A. , Putney Ryan M. , Ajidahun Abidemi O. , Coppola Domenico , Shibata David 
Published: December 2014

Abstract
Background: Epigenome-wide association studies are emerging in the field of cancer epidemiology with the rapid development of large-scale methylation array platforms. Until recently, these methods were only valid for DNA from flash frozen (FF) tissues. Novel techniques for repairing DNA from formalin-fixed paraffin-embedded (FFPE) tissues have emerged; however, a direct comparison of FFPE DNA repair methods before analysis on genome-wide methylation array to matched FF tissues has not been conducted.
Methods: We conducted a systematic performance comparison of two DNA repair methods (REPLI-g Ligase vs. Infinium HD Restore Kit) on FFPE-DNA compared with matched FF tissues on the Infinium 450K array. A threshold of discordant methylation between FF-FFPE pairs was set at Δβ &gt; 0.3. The correlations of β-values from FF–FFPE pairs were compared across methods and experimental conditions.
Results: The Illumina Restore kit outperformed the REPLI-g ligation method with respect to reproducibility of replicates (R2 &gt; 0.970), highly correlated β-values between FF-FFPE (R2 &gt; 0.888), and fewest discordant loci between FF-FFPE (≤0.61%). The performance of the Restore kit was validated in an independent set of 121 FFPE tissues.
Conclusions: The Restore kit outperformed RELPI-g ligation in restoring FFPE-derived DNA before analysis on the Infinium 450K methylation array. Our findings provide critical guidance that may significantly enhance the breadth of diseases that can be studied by methylomic profiling.
Impact: Epigenomic studies using FFPE tissues should now be considered among cancers that have not been fully characterized from an epigenomic standpoint. These findings promote novel epigenome-wide studies focused on cancer etiology, identification of novel biomarkers, and developing targeted therapies.
See all the articles in this CEBP Focus section, “Biomarkers, Biospecimens, and New Technologies in Molecular Epidemiology.”
Cancer Epidemiol Biomarkers Prev; 23(12); 2622–31. ©2014 AACR.
Methods for Sample Acquisition and Processing of Serial Blood and Tumor Biopsies for Multicenter Diffuse Large B-cell Lymphoma Clinical Trials
Authors: Nielsen Torsten Holm , Diaz Zuanel , Christodoulopoulos Rosa , Charbonneau Fredrick , Qureshi Samia , Rousseau Caroline , Benlimame Naciba , Camlioglu Errol , Constantin André Marc , Oros Kathleen Klein , Krumsiek Jan , Crump Michael , Morin Ryan D. , Cerchietti Leandro , Johnson Nathalie A. , Petrogiannis-Haliotis Tina , Miller Wilson H. , Assouline Sarit E. , Mann Koren K. 
Published: December 2014

Abstract
Increasingly, targeted therapies are being developed to treat malignancies. To define targets, determine mechanisms of response and resistance, and develop biomarkers for the successful investigation of novel therapeutics, high-quality tumor biospecimens are critical. We have developed standard operating procedures (SOPs) to acquire and process serial blood and tumor biopsies from patients with diffuse large B-cell lymphoma enrolled in multicenter clinical trials. These SOPs allow for collection and processing of materials suitable for multiple downstream applications, including immunohistochemistry, cDNA microarrays, exome sequencing, and metabolomics. By standardizing these methods, we control preanalytic variables that ensure high reproducibility of results and facilitate the integration of datasets from such trials. This will facilitate translational research, better treatment selection, and more rapid and efficient development of new drugs.
See all the articles in this CEBP Focus section, “Biomarkers, Biospecimens, and New Technologies in Molecular Epidemiology.”
Cancer Epidemiol Biomarkers Prev; 23(12); 2688–93. ©2014 AACR.
Effects of Preanalytic Variables on Circulating MicroRNAs in Whole Blood
Authors: Zhao Hua , Shen Jie , Hu Qiang , Davis Warren , Medico Leonardo , Wang Dan , Yan Li , Guo Yuqing , Liu Biao , Qin Maochun , Nesline Mary , Zhu Qianqian , Yao Song , Ambrosone Christine B. , Liu Song 
Published: December 2014

Abstract
Research in the last decade suggests the clinical potential of circulating microRNAs in whole blood as biomarkers for cancer detection. However, before applying the identified circulating microRNAs clinically, biospecimen-focused research has to be performed to identify possible preanalytic variables that may significantly affect the levels of circulating microRNAs. In this study, using a unique resource of the Data Bank and BioRepository (DBBR) at Roswell Park Cancer Institute, we conducted a two-step analysis to identify internal control circulating microRNAs in whole blood and then to study how selected major preanalytic variables (namely, processing delay, storage condition, storage time, and freeze/thaw cycles) might affect the detection of circulating microRNAs. In the discovery phase of the first step, we identified three microRNAs, including miR346, miR134, and miR934, whose levels exhibited the smallest variation between the case–control groups, as well as within each group interindividually. In the further validation analysis, the consistency was validated for miR346 and miR134 but not for miR934. At the second step, using miR346 and miR134 as internal controls, we observed that as the numbers of freeze/thaw cycles increased, levels of both miR346 and miR134 were significantly decreased (Ptrend &lt; 0.0001); varying other processing and storage conditions did not affect miRNA levels. In the paralleled analysis in plasma samples, levels of miR16 were significantly decreased by increasing processing delay and increasing numbers of freeze/thaw cycles but not affected by storage condition and duration. The results from this study highlight the necessity of biospecimen-focused research on circulating microRNAs before clinical utilization.
See all the articles in this CEBP Focus section, “Biomarkers, Biospecimens, and New Technologies in Molecular Epidemiology.”
Cancer Epidemiol Biomarkers Prev; 23(12); 2643–8. ©2014 AACR.
Circulating Sex Hormones and Terminal Duct Lobular Unit Involution of the Normal Breast
Authors: Khodr Zeina G. , Sherman Mark E. , Pfeiffer Ruth M. , Gierach Gretchen L. , Brinton Louise A. , Falk Roni T. , Patel Deesha A. , Linville Laura M. , Papathomas Daphne , Clare Susan E. , Visscher Daniel W. , Mies Carolyn , Hewitt Stephen M. , Storniolo Anna Maria V. , Rosebrock Adrian , Caban Jesus J. , Figueroa Jonine D. 
Published: December 2014

Abstract
Background: Terminal duct lobular units (TDLU) are the predominant source of breast cancers. Lesser degrees of age-related TDLU involution have been associated with increased breast cancer risk, but factors that influence involution are largely unknown. We assessed whether circulating hormones, implicated in breast cancer risk, are associated with levels of TDLU involution using data from the Susan G. Komen Tissue Bank (KTB) at the Indiana University Simon Cancer Center (2009–2011).
Methods: We evaluated three highly reproducible measures of TDLU involution, using normal breast tissue samples from the KTB (n = 390): TDLU counts, median TDLU span, and median acini counts per TDLU. RRs (for continuous measures), ORs (for categorical measures), 95% confidence intervals (95% CI), and Ptrends were calculated to assess the association between tertiles of estradiol, testosterone, sex hormone–binding globulin (SHBG), progesterone, and prolactin with TDLU measures. All models were stratified by menopausal status and adjusted for confounders.
Results: Among premenopausal women, higher prolactin levels were associated with higher TDLU counts (RRT3vsT1:1.18; 95% CI: 1.07–1.31; Ptrend = 0.0005), but higher progesterone was associated with lower TDLU counts (RRT3vsT1: 0.80; 95% CI: 0.72–0.89; Ptrend &lt; 0.0001). Among postmenopausal women, higher levels of estradiol (RRT3vsT1:1.61; 95% CI: 1.32–1.97; Ptrend &lt; 0.0001) and testosterone (RRT3vsT1: 1.32; 95% CI: 1.09–1.59; Ptrend = 0.0043) were associated with higher TDLU counts.
Conclusions: These data suggest that select hormones may influence breast cancer risk potentially through delaying TDLU involution.
Impact: Increased understanding of the relationship between circulating markers and TDLU involution may offer new insights into breast carcinogenesis. Cancer Epidemiol Biomarkers Prev; 23(12); 2765–73. ©2014 AACR.
Arrhythmia Burden in Elderly Patients With Severe Aortic Stenosis as Determined by Continuous Electrocardiographic Recording
Authors: Urena Marina , Hayek Salim , Cheema Asim N. , Serra Vicenç , Amat-Santos Ignacio J. , Nombela-Franco Luis , Ribeiro Henrique B. , Allende Ricardo , Paradis Jean-Michel , Dumont Eric , Thourani Vinod H. , Babaliaros Vasilis , Francisco Pascual Jaume , Cortés Carlos , del Blanco Bruno García , Philippon François , Lerakis Stamatios , Rodés-Cabau Josep 
Published: December 2014

Background—
This study sought to evaluate the prevalence of previously undiagnosed arrhythmias in candidates for transcatheter aortic valve replacement (TAVR) and to determine the impact on therapy changes and arrhythmic events after the procedure.

Methods and Results—

A total of 435 candidates for TAVR underwent 24-hour continuous ECG monitoring the day before the procedure. Newly diagnosed arrhythmias were observed in 70 patients (16.1%) before TAVR: paroxysmal atrial fibrillation (AF)/atrial tachycardia (AT) in 28, advanced atrioventricular block or severe bradycardia in 24, nonsustained ventricular tachycardia in 26, and intermittent left bundle-branch block in 3 patients. All arrhythmic events but one were asymptomatic and led to a therapy change in 43% of patients. In patients without known AF/AT, the occurrence of AF/AT during 24-hour ECG recording was associated with a higher rate of 30-day cerebrovascular events (7.1% versus 0.4%;
P
=0.030). Among the 53 patients with new-onset AF/AT after TAVR, 30.2% had newly diagnosed paroxysmal AF/AT before the procedure. In patients who needed permanent pacemaker implantation after the procedure (n=35), 31.4% had newly diagnosed advanced atrioventricular block or severe bradycardia before TAVR. New-onset persistent left bundle-branch block after TAVR occurred in 37 patients, 8.1% of whom had intermittent left bundle-branch block before the procedure.

Conclusions—
Newly diagnosed arrhythmias were observed in approximately a fifth of TAVR candidates, led to a higher rate of cerebrovascular events, and accounted for a third of arrhythmic events after the procedure. This high arrhythmia burden highlights the importance of an early diagnosis of arrhythmic events in such patients to implement the appropriate therapeutic measures earlier.
Cortical Microinfarcts Detected In Vivo on 3 Tesla MRI
Authors: van Dalen Jan Willem , Scuric Eva E.M. , van Veluw Susanne J. , Caan Matthan W.A. , Nederveen Aart J. , Biessels Geert Jan , van Gool Willem A. , Richard Edo 
Published: December 2014

Background and Purpose—
Cortical microinfarcts (CMIs) are a common postmortem finding associated with vascular risk factors, cognitive decline, and dementia. Recently, CMIs identified in vivo on 7 Tesla MRI also proved retraceable on 3 Tesla MRI.

Methods—
We evaluated CMIs on 3 Tesla MRI in a population-based cohort of 194 nondemented older people (72–80 years) with systolic hypertension. Using a case–control design, participants with and without CMIs were compared on age, sex, cardiovascular risk factors, and white matter hyperintensity volume.

Results—
We identified 23 CMIs in 12 participants (6%). CMIs were associated with older age, higher diastolic blood pressure, and a history of recent stroke. There was a trend for a higher white matter hyperintensity volume in participants with CMIs.

Conclusions—
We found an association of CMIs with clinical parameters, including age and cardiovascular risk factors. Although the prevalence of CMIs is relatively low, our results suggest that the study of CMIs in larger clinical studies is possible using 3 Tesla MRI. This opens the possibility of large-scale prospective investigation of the clinical relevance of CMIs in older people.
Scaling Behaviors of Global Sea Surface Temperature
Authors: Luo Ming , Leung Yee , Zhou Yu , Zhang Wei 
Published: December 2014

AbstractTemporal scaling properties of the monthly sea surface temperature anomaly (SSTA) in global ocean basins are examined by the power spectrum and detrended fluctuation analysis methods. Analysis results show that scaling behaviors of the SSTA in most ocean basins (e.g., global average, South Pacific, eastern and western tropical Pacific, tropical Indian Ocean, and tropical Atlantic) are separated into two distinct regimes by a common crossover time scale of 52 months (i.e., 4.3 yr). It is suggested that this crossover is modulated by the El Niño/La Niña–Southern Oscillation (ENSO), indicating different scaling properties at different time scales. The SSTA time series is nonstationary and antipersistent at the small scale (i.e., crossover). It is, however, stationary and long range correlated at the large scale (i.e., crossover). For both time scales, scaling behaviors of SSTA are heterogeneously distributed over the ocean, and the fluctuation of SSTA intensifies with decreasing latitude. Stronger fluctuation appears over the tropical regions (e.g., central-eastern tropical Pacific, tropical Atlantic, tropical Indian Ocean, and South China Sea), which are directly or indirectly linked to ENSO. Weaker fluctuation and stronger persistence are found in mid- and high-latitude areas, coinciding with the “reemergence” areas.
Enhancing Coping and Supporting Protective Factors After a Disaster
Authors: Powell Tara , Thompson Sanna J. 
Published: December 2014

Objective: This article presents the Journey of Hope (JoH), a school-based intervention for children who have experienced a collective trauma such as a natural disaster. Through the use of group work, the JoH focuses on building coping skills and enhancing protective factors to help children recover. Method: This quasi-experimental research included 102 children impacted by tornadoes in Tuscaloosa, Alabama in 2011. Results: Through an hierarchical linear model analysis with ( n = 48) from the JoH group and ( n = 54) from a wait-list control group, the outcomes indicate that after participation in the JoH youth had increased coping skills, F(100) = 5.270, p < .05, and prosocial behaviors, F(95) = 4.286, p < .05. This is the first quasi-experimental design to be conducted on the JoH; findings provide preliminary evidence of the effectiveness of this broad-based postdisaster intervention. Conclusion: Future replication studies with larger samples in other societies impacted by a natural disaster are needed to further evaluate the JoH’s impact in enhancing coping and building resilience.
Conflict adaptation and congruency sequence effects to social–emotional stimuli in individuals with autism spectrum disorders
Authors: Worsham Whitney , Gray Whitney E , Larson Michael J , South Mikle 
Published: December 2014

Background: The modification of performance following conflict can be measured using conflict adaptation tasks thought to measure the change in the allocation of cognitive resources in order to reduce conflict interference and improve performance. While previous studies have suggested atypical processing during nonsocial cognitive control tasks, conflict adaptation (i.e. congruency sequence effects) for social–emotional stimuli have not been previously studied in autism spectrum disorder. Methods: A total of 32 participants diagnosed with autism spectrum disorder and 27 typically developing matched controls completed an emotional Stroop conflict task that required the classification of facial affect while simultaneously ignoring an overlaid affective word. Results: Both groups showed behavioral evidence for emotional conflict adaptation based on response times and accuracy rates. However, the autism spectrum disorder group demonstrated a speed-accuracy trade-off manifested through significantly faster response times and decreased accuracy rates on trials containing conflict between the emotional face and the overlaid emotional word. Conclusion: Reduced selective attention toward socially relevant information may bias individuals with autism spectrum disorder toward more rapid processing and decision making even when conflict is present. Nonetheless, the loss of important information from the social stimuli reduces decision-making accuracy, negatively affecting the ability to adapt both cognitively and emotionally when conflict arises.
Modulation of tumor fatty acids, through overexpression or loss of thyroid hormone responsive protein spot 14 is associated with altered growth and metastasis
Authors: Wellberg Elizabeth A , Rudolph Michael C , Lewis Andrew S , Padilla-Just Nuria , Jedlicka Paul , Anderson Steven M 
Published: December 2014

Abstract
Introduction
Spot14 (S14), encoded by the THRSP gene, regulates de novo fatty acid synthesis in the liver, adipose, and lactating mammary gland. We recently showed that S14 stimulated fatty acid synthase (FASN) activity in vitro, and increased the synthesis of fatty acids in mammary epithelial cells in vivo. Elevated de novo fatty acid synthesis is a distinguishing feature of many solid tumors compared with adjacent normal tissue. This characteristic is thought to be acquired during tumor progression, as rapidly proliferating cells have a heightened requirement for membrane phospholipids. Further, overexpression of FASN is sufficient to stimulate cell proliferation. While many studies have focused on the FASN enzyme in cancer biology, few studies have addressed the roles of proteins that modify FASN activity, such as S14.

Methods
Tumor fatty acids were modulated using two mouse models, mouse mammary tumor virus (MMTV)-neu mice overexpressing S14 and MMTV-polyomavirus middle T antigen (PyMT) mice lacking S14, and associations between elevated or impaired fatty acid synthesis on tumor latency, growth, metastasis, and signaling pathways were investigated. We evaluated S14-dependent gene expression profiles in mouse tumors by microarray and used publicly available microarray datasets of human breast tumors.

Results
S14 overexpression in the MMTV-Neu transgenic model is associated with elevated medium-chain fatty acids, increased proliferation and a shorter tumor latency, but reduced tumor metastasis compared to controls. Loss of S14 in the MMTV-PyMT model decreased FASN activity and the synthesis of medium-chain fatty acids but did not alter tumor latency. Impaired fatty acid synthesis was associated with reduced solid tumor cell proliferation, the formation of cystic lesions in some animals, and decreased phosphorylation of Src and protein kinase B (Akt). Analysis of gene expression in these mouse and human tumors revealed a relationship between S14 status and the expression of genes associated with luminal epithelial differentiation.

Conclusions
This study demonstrates a potential role for S14 in regulating mammary tumor growth and fatty acid synthesis in vivo. Furthermore, these results suggest that modulating the amount of medium chain fatty acids, by changing the levels of S14, has the potential to impact malignant mammary tumor phenotypes.