bluuebunny/crossref_metadata_2025_split huggingface dataset:
L-Carnitine sustainably affects bioenergetic profile of bovine blastocysts and transcriptome profile of elongation-stage embryos
Authors: Held-Hoelker Eva , Kurzella Jessica , Salilew-Wondim Dessie , Rings Franca , Tesfaye Dawit , Tholen Ernst , Grosse-Brinkhaus Christine , Hoelker Michael 
Published: June 2024

In brief
In the present study the sustainable effect of L-carnitine during the culture period on the post-transfer development was investigated. Taken together, we uncovered direct effects of L-carnitine on the bioenergetic profile of day 7 blastocysts along with sustainable effects on mtDNA copy numbers and transcriptome profile of bovine day 14 embryos.

Abstract
L-Carnitine (LC) is known to play key roles in lipid metabolism and antioxidative activity, implicating enhanced cryotolerance of bovine blastocysts. However, sustainability of LC supplementation during culture period on preimplantation development beyond the blastocyst stage has not been investigated so far. Therefore, all embryos were cultured under fatty acid-free conditions, one group with LC (LC embryos) and the control group without LC (control) supplementation. Transfer to recipients was conducted on day 6. Elongation-stage embryos were recovered on day 14; metrics of embryo recollection, developmental rates as regards early elongation-stage as well as mean embryo length did not differ between the groups. Gene expression analyses via NGS revealed 341 genes to be differentially regulated between elongation-stage embryos derived from LC supplementation compared to controls. These played mainly a role in molecular functions and biological processes like oxidoreductase activity, ATP-dependent activity, cellular stress, and respiration. Pathways like oxidative phosphorylation and thermogenesis, extracellular matrix receptor signaling, PI3K-Akt, and focal adhesion were affected by differentially regulated genes. Moreover, all DEGs located on the mitochondria were significantly downregulated in LC embryos, being in line with lower mitochondrial copy number and mtDNA integrity compared to the control group. Finally, we uncovered alterations of the bioenergetic profile on day 7 as a consequence of LC supplementation for the first time, revealing significantly higher oxygen consumption rates, ATP linked respiration and spare capacity for LC embryos. In summary, we uncovered direct effects of LC supplementation during the culture period on the bioenergetic profile along with sustainable effects on mtDNA copy numbers and transcriptome profile of bovine day 14 embryos.
To Cover or to Hide? The Role of the Media in the Trials of the Nazis and Their Collaborators
Authors: Vaiseta Tomas 
Published: June 2024

Rec.: Ruth Leiserowitz, Gintarė Malinauskaitė, Hektoras Vitkus (eds.), Making Justice Visible: War Crimes Trials, Media and Memory after World War II, Osnabrück: fibre Verlag, 2022.
Evaluating Cardiorespiratory Fitness Level of College Students: A Comparative Analysis Between Basketball and Football Classes
Authors: Arrum Dian Noer Anggita , Tomoliyus  , Alim Abdul , Miftachurochmah Yulvia 
Published: June 2024

Objectives. The study aimed to compare the cardiorespiratory fitness levels among male students majoring in football and basketball.
Materials and methods. A cross-sectional study was conducted with a sample size of 60 students, divided into two groups: 30 students from the basketball class and 30 students from the football class. The average age of students in the football class was 22.03 ± 0.81, while in the basketball class, it was 22.07 ± 0.74. All participants were male and active strata 1 students in semesters 4 and 6, equivalent to second and third-year students. The cardiorespiratory fitness level was assessed using the Cooper 1.5 mile or 2.4 km Run Test. The data analysis was performed using a two-tailed independent sample t-test to determine differences between the two groups at a significance level of less than 5% (p<0.05). Prior to the t-test, normality was assessed using the Shapiro-Wilk test, and homogeneity was assessed using Levene’s statistics, with a significance level higher than 5% (p>0.05).
Results. The findings revealed that students in the football class showed a higher VO₂max (42.54 ± 5.40) compared to those in the basketball class (37.86 ± 5.62), demonstrating a statistically significant difference (p = 0.002).
Conclusions. The study found a significant disparity in cardiorespiratory fitness levels between the football and basketball student groups within the campus setting.
Jovens cientistas: enfrentamento às arboviroses e participação juvenil no território
Authors: Padrão Maria Regina Araújo de Vasconcelos , Da Rocha Fernando Gomes , Siqueira Mariana , Garcez Loureine Rapôso Oliveira , Mourão Caroline Barbosa Farias , Cavaca Aline Guio , Sepúlveda Luciana 
Published: June 2024

This study analyzes a Scientific Initiation (SI) program on arboviroses, from the perception of young high school students in Ceilândia – Distrito Federal. Objective: It aims to understand to what extent the SI mobilizes these young people to participate in surveillance and health promotion actions in the territory and understand their motivation to participate in this project. Methodology: The methodology included the analysis of the essays written for the selection to the Training Course for Scientific Initiation in Basic Education and the Arboviroses, the administration of this course, and later a focus group with students to analyze the SI educational process they experienced. Results: The result of the Content Analysis showed that the students’ perception about the SI could be grouped into six categories: students’ motivation for the scientific initiation; the learning process; the training of young scientists; health and environment; youth participation and empowerment, and perceptions of the Public health system in Brazil (SUS). Conclusion: Confronting arboviroses requires broad-spectrum policies and interventions, and the SI strengthened these young students as subjects of rights, broadened their perception of science, health, relationship with the environment, with the Sistema Único de Saúde and stimulated interest in preventive actions to combat arboviroses in these territories.
Submission: 12/19/23| Review: 03/27/24| Approval: 04/24/24
O cuidado interprofissional na Atenção Primária à Saúde
Authors: Vieira Levandovski Cristina , Pekelman Renata 
Published: June 2024

Objetivo: identificar a interprofissionalidade no cuidado em saúde realizado por profissionais de uma unidade de saúde de Porto Alegre, Rio Grande do Sul, após o contexto de agudização da pandemia de Covid-19. Método: pesquisa qualitativa de tipo descritivo-exploratória. A observação participante e o registro em diário de campo foram as técnicas de pesquisa utilizadas. Ao total realizam-se quatro observações, sendo duas em cada uma das equipes de referência escolhidas. Resultados: a interprofissionalidade está associada a uma forma de agir no trabalho em saúde, sendo algo que se estabelece de modo processual a partir das relações entre os (as) trabalhadores (as) e com a população usuária. As duas equipes de referência possuem preocupação com a concretização da atenção centrada nas necessidades do (a) usuário (a), sendo isso uma das características do trabalho em equipe a partir da perspectiva interprofissional. Contudo, há empecilhos para efetivação da interprofissionalidade no cuidado em saúde, relacionado à organização da reunião de equipe de referência, ao entendimento de cada trabalhador (a) acerca de sua contribuição nesse espaço e ao cenário atual da atenção primária à saúde. Conclusões: A interprofissionalidade esteve presente nas reuniões de equipe de referência observadas. Por outro lado, as alterações no processo de trabalho decorrentes do contexto de pandemia de Covid-19 foram identificadas como limitações para a efetivação da interprofissionalidade no cuidado em saúde.

Palavras-chave: Trabalho em equipe; Interprofissionalidade; Cuidado em saúde; Atenção Primária à saúde.
FAKTOR YANG MEMPENGARUHI PERSEPSI MAHASISWA MENGENAI PRAKTIK CREATIVE ACCOUNTING DENGAN RELIGIUSITAS ISLAMI SEBAGAI PEMODERASI
Authors: Masrika Puspa Desi 
Published: June 2024

Penelitian ini bertujuan untuk mengetahui faktor- faktor yang mempengaruhi persepsi mahasiswa akuntansi angkatan 2018-2020 Fakultas Ekonomi dan Bisnis Universitas Muhammadiyah Riau mengenai Creative Accounting. Penelitian ini adalah penelitian kuantitatif. Populasi penelitian ini ialah mahasiswa akuntansi angkatan 2018-2020 yang berada di Universitas Muhammadiyah Riau. Populasi penelitian ini sebanyak 640 mahasiswa, dan sampel penelitian ini sebanyak 314 responden dengan teknik pengambilan sampel yaitu teknik purposive sampling. Pengumpulan data penelitian ini dengan cara penyebaran kuesioner kepada responden melalui Google Form. Analisis data yang digunakan penelitian ini ialah uji statistik dekriptif, uji asumsi klasik dan uji hipotesis dengan alat bantu SPSS 22. Hasil penelitian ini menunjukkan bahwa pengetahuan etika profesi akuntan dan orientasi etis berpengaruh terhadap persepsi mahasiswa akuntansi mengenai creative accounting. Hasil penelitian ini juga menunjukkan bahwa religiusitas islami memoderasi orientasi etis mengenai persepsi mahasiswa akuntansi mengenai creative accounting. Sedangkan, religiusitas islami bukan varibel pemoderasi orientasi etis mengenai persepsi mahasiswa akuntansi mengenai creative accounting.
The Interplay among Wnt/β-Catenin Family Members in Colorectal Adenomas and Surrounding Tissues
Authors: D'Antonio Domenica Lucia , Fantini Fabiana , Moscatello Carmelo , Alessio Ferrone , Scaringi Stefano , Valanzano Rosa , Ficari Ferdinando , Efthymakis Konstantinos , Neri Matteo , Aceto Gitana Maria , Curia Maria Cristina 
Published: June 2024

Background: Colorectal adenoma undergoes neoplastic progression via the normal epithelium-adenoma-adenocarcinoma sequence as reported in the Vogelgram. The risk of developing cancer is strongly associated with the number and size of adenoma and with the subtype. Currently, adenomatous polyps can be distinguished on the basis of histology: the prevalence are tubular, 5-15% are villous and tubular/villous. Considering the increased risk for malignant transformation described for tubular/villous adenomas, patients diagnosed with adenomatous polyposis are at increased risk of developing CRC. The Wnt/β-catenin pathway plays a key role in the onset of colorectal adenoma, in particular intestinal cells first acquire loss-of-function mutations in APC gene that induce the formation of adenomas.
Methods: Wnt/β-catenin pathway APC, Wnt3a, Wnt5a, LEF1, BCL9 genes and protein expression analyses were conducted by qRT-PCR and western blot in 68 colonic samples (polyps and adjacent mucosa) from 41 patients, of which 17 affected by FAP. Ten normal colonic mucosal samples were collected from 10 healthy donors.
Results: In this study both APC gene and protein resulted less expressed in colon tumor compared to the adjacent colonic mucosa. Conversely, activated β-catenin was more expressed in polyps than in the adjacent mucosa. All results confirmed literature data on carcinomas. A statistically significant correlation between Wnt3a and BCL9 both in polyps and in the adjacent mucosa underlies that the canonical Wnt pathway is activated in early colon carcinogenesis and that the adjacent mucosa is already altered.
Conclusion: This is the first study analyzing the difference in expression of Wnt/β-catenin pathway in human colorectal adenomas. Understanding the progression from adenomas to colorectal carcinomas is essential for the development of new therapeutic strategies and improving clinical outcomes.
Additional feedforward mechanism of Parkin activation via binding of phospho-UBL and RING0 in trans
Authors: Lenka Dipti , Dahe Shakti , Antico Odetta , Sahoo Pritiranjan , Prescott Alan R. , Muqit Miratul M. K. , Kumar Atul 
Published: June 2024

Abstract

Loss of function Parkin mutations lead to early-onset of Parkinson’s disease. Parkin is an auto-inhibited ubiquitin E3 ligase activated by dual phosphorylation of its ubiquitin-like (Ubl) domain and ubiquitin by the PINK1 kinase. Herein, we demonstrate a competitive binding of the phospho-Ubl and RING2 domains towards the RING0 domain, which regulates Parkin activity. We show that phosphorylated Parkin can complex with native Parkin, leading to the activation of autoinhibited native Parkin in trans. Furthermore, we show that the activator element (ACT) of Parkin is required to maintain the enzyme kinetics, and the removal of ACT slows the enzyme catalysis. We also demonstrate that ACT can activate Parkin in trans but less efficiently than when present in the cis molecule. Furthermore, the crystal structure reveals a donor ubiquitin binding pocket in the linker connecting REP and RING2, which plays a crucial role in Parkin activity.
A rapid aureochrome opto-switch enables diatom acclimation to dynamic light
Authors: Li Xiaobo , Zhang Huan , Xiong Xiaofeng , Guo Kangning , Zheng Mengyuan , Cao Tianjun , Yang Yuqing , Song Jiaojiao , Cen Jie , Zhang Jiahuan , Jiang Yanyou , Feng Shan , Tian Lijin 
Published: June 2024

Abstract

Diatoms often outnumber other eukaryotic algae in the oceans, especially in coastal environments characterized by frequent fluctuations in light intensity. The identities and operational mechanisms of regulatory factors governing diatom acclimation to high light stress remain largely elusive. Here, we identified the AUREO1c protein from the coastal diatom Phaeodactylum tricornutum as a crucial regulator of non-photochemical quenching (NPQ), a photoprotective mechanism that dissipates excess energy as heat. AUREO1c detects light stress using a light-oxygen-voltage (LOV) domain and directly activates the expression of target genes, including LI818 genes that encode NPQ effector proteins, via its bZIP DNA-binding domain. In comparison to a kinase-mediated pathway reported in the freshwater green alga Chlamydomonas reinhardtii, the AUREO1c pathway exhibits a faster response and enables accumulation of LI818 transcript and protein levels to comparable degrees between continuous high-light and fluctuating-light treatments. We propose that the AUREO1c-LI818 pathway contributes to the resilience of diatoms under dynamic light conditions.
Effects of water surface photovoltaic systems on the water physiochemical parameters and biodiversity
Authors: Ma Zhijun , Yang Sen , Zhang Youzheng , Tian Dengfeng 
Published: June 2024

Abstract

The implementation of water surface photovoltaic (WSPV) systems as a source of renewable power has expanded rapidly worldwide in recent decades. WSPV prevents negative impacts on terrestrial ecosystems, while the impacts on aquatic physiochemical traits and biodiversity are unclear. To understand the ecological and environmental impacts of WSPV systems, we conducted a field survey on water physicochemical parameters, plankton, and birds of 26 WSPV systems in the Yangtze River basin in China during the winter and summer of 2022. We found that WSPV systems decreased water temperature, dissolved oxygen and uncovered area of the water surface, which caused a reduction in species richness and individual density and altered the community composition of plankton. WSPV systems also caused an overall decrease in bird diversity and changed bird community compositions. These findings suggested that WSPV systems have long-term impacts on the water ecology and environment.
Using a barrier analysis to identify determinants of dietary diversity among adult men and women in rural Odisha, India
Authors: Sivalogan Kasthuri , Salinger Allison P. , Kalam Md. Abul , Bisoyi Alokananda , Chapnick Melissa , Clasen Thomas F. , Sinharoy Sheela S. 
Published: June 2024

Abstract

Introduction: Dietary diversity, a predictor of micronutrient adequacy, is low in rural Odisha, India. We conducted formative research to understand determinants of dietary diversity and to inform the design of an agriculture-nutrition intervention in rural Odisha.
Methods: We conducted a sequential mixed-method study in June-July 2022. First, an abbreviated 24-hour dietary recall was conducted with 429 respondents from 11 villages to capture food consumption patterns. Then, a barrier analysis was conducted among 41 doers, who consumed ≥5 of 10 food groups, and 40 non-doers, who consumed <5 food groups, to identify enablers and barriers to dietary diversity. Closed-ended responses were analyzed using Fishers exact test. Open-ended responses were analyzed using rapid qualitative methods.
Results: Participants in the barrier analysis included men and women from indigenous and non-indigenous groups. Most doers (88%) reported consuming 5 food groups in the past 24 hours. Non-doers reported consuming 2-4 food groups with almost half (47.5%) consuming 3 food groups in the past 24 hours. Accessibility to non-governmental policies, programmes or information channels was the only significant determinant of dietary diversity [p=0.016]. Qualitative results indicated that self-efficacy, access and affordability, perceived consequences of a diverse diet, and dietary practices associated with religious and cultural events influenced dietary diversity.
Conclusion: These results indicate that individual, market, socio-cultural, and policy level factors were all determinants of dietary diversity in rural Odisha. Findings will be used to design a homestead food production intervention to target determinants for improved nutrition.
Proper ink management impact on Overall Environmental Equipment Efficiency for Sustainability – a case study for the printing company
Authors: Krystosiak Krzysztof , Kluczek Aldona , Werpachowski Wojciech 
Published: June 2024

Abstract

Printing as a process itself generates many environmental concerns. The paper presents the subject matter of ink management in terms of environmental issues in the label printing industry. The goal of this paper is to present how a proper ink management system has an impact on the overall printing process efficiency for environmental sustainability in a printing company.
A case study-based methodology has been used to depict the impact of ink management on the overall equipment effectiveness (OEE) in terms of the material quantity and costs of a typical printed job. Results have shown a positive aspect of sustainability in the printing process due to the decrease of wasted inks, printing substrates, and energy.
A modified approach to the management of components to produce labels and packaging has been presented using automatic ink dispensing systems in companies producing labels and packaging. Proper ink management in a printing house might provide significant effects in the following areas: efficiency, quality, environmental impact, and social aspects. In addition, potential future directions, and the practical implications for supporting operational excellence on production in manufacturing have also been described. Through the case study, this research has turned attention to the reality of company operations at the intersection of sustainability and industry.
JEL Classifications L0, L6, L8, L9
Cuff Shaving Procedure for Distal Cuff Erosion in Peritoneal Dialysis Patients – Three-Year Follow-up
Authors: Maroz Natallia , Gilkeson Julie , Kamel Tony 
Published: June 2024

Abstract

Background
Erosion of the distal cuff of peritoneal dialysis (PD) catheters can lead to exit site infections, tunnel infections, peritonitis, and often culminates in catheter loss, necessitating catheter replacement or permanent transition to hemodialysis (HD). Surgical shaving of the distal cuff has been reported as an alternative option for management of distal cuff erosion. In this paper, we report promising results of a three year follow up study of a patient with distal cuff erosion who was treated with surgical shaving of the distal cuff.
Methods
We performed a retrospective review for a patient with end stage renal disease (ESRD) who uses PD, who had documented subclinical erosion of the PD cuff and was managed with surgical shaving of the eroded portion. The PD catheter was originally inserted by a vascular surgeon with the outer cuff implanted subcutaneously, approximately 2–3 cm away from the exit site. The patient did not experience any post-surgical complications following catheter insertion. The PD catheter was in use for more than one year prior to erosion. Shaving of the distal cuff was performed and the patient’s case was reviewed over a period of 3 years.
Results
We identified a patient with distal cuff erosion for whom the cause of erosion was documented as mechanical force. The patient was noted to have normal function of their PD catheter at the time of diagnosis without any signs or symptoms of infection. Over a period of 3 years after cuff shaving, there were no episodes of exit-site infection, tunnel infection, or peritonitis. The patient experienced excellent catheter function following surgical shaving, and throughout follow up.
Conclusion
Shaving of the distal Dacron cuff is a viable option for the management of select peritoneal dialysis patients with erosion of the distal cuff of the catheter. This treatment modality has the potential to reduce the incidence of catheter related infections, spare patients from surgical complications related to catheter removal and replacement, and prevent a permanent transition to hemodialysis due to cuff erosion.
Comparison of the safety of flexible ureteroscopy with the different irrigation methods in a 3D print kidney model
Authors: Bai Baohua , Jiang Shuangjian , Zhang Junlong , Zeng Qinsong , Mo Chengqiang , Wu Rongpei 
Published: June 2024

Abstract

Objective
To compare intrarenal pressure(IRP) and irrigation flow at various suspended water heights and use hand pumping during flexible ureteroscopy in an ex vivo 3D print kidney model.
Methods
A 3D print silica gel model was used to simulate the kidney. The ureteral access sheath(UAS) was connected to the kidney model and positioned at the ureteropelvic junction. Central venous pressure tubing was connected to monitor the pressure in the renal pelvis under different conditions. 12Fr and 14Fr sheath sizes were tested with different flexible ureteroscope(fURS) sizes of 7.5, 8.5, and 9.5Fr, respectively. The irrigation was gravity-based, and the hung water heights were set at 60, 90, 120, 150, and 180 cm. A manual pumping group was also measured.
Results
Using a 12Fr sheath with a 9.5Fr fURS loading with no accessories resulted in IRP ranging from 8.4–17.5 cmH2O while manual pumping perfusion pressure exceeded 60 cmH2O. Loading a 200um laser fiber decreased the pressure to 6.4–10.5 cmH2O and 4.0–5.0 cmH2O when loading a stone basket. Using a 14Fr sheath with a 9.5Fr fURS resulted in an IRP of 2.5-6.0 cmH2O, compared to 17cmH2O with manual pumping. With a 12Fr sheath and a 7.5Fr fURS, the IRP ranged from 5.4–8.2 cmH2O, while manual pumping resulted in 25.5 cmH2O. With a 14Fr sheath and a 7.5Fr fURS, the IRP ranged from 1.5-4.3cmH2O, while manual pumping resulted in 9.0 cmH2O.
Conclusion
When using a UAS in a flexible ureteroscopy, the IRP can be maintained in a safe range with different fURS/UAS combos with a suspended water height of less than 180cm. While in specific fURS/UAS(9.5Fr/12Fr) combos, the IRP exceeded the safe limit when using manual pumping. Gravity irrigation of less than 180cm hung water height is safe in this simulated clinical experiment environment.