bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental exposure of eggs to polybrominated diphenyl ethers BDE-47 and BDE-99 in red-eared sliders (Trachemys scripta elegans) and snapping turtles (Chelydra serpentina) and possible species-specific differences in debromination
Authors: Eisenreich Karen M. , Rowe Christopher L. 
Published: November 2012

Abstract
Polybrominated diphenyl ethers (PBDEs) are a bioaccumulative, persistent, and toxic class of flame retardants that can potentially impact turtles in natural habitats via exposure through maternal transfer. To simulate maternal transfer in the present study, PBDE congeners BDE-47 and BDE-99 were topically applied to the eggshell and were allowed to diffuse into the egg contents of the red-eared slider (Trachemys scripta elegans) and snapping turtle (Chelydra serpentina). Eggs were topically dosed over 8 d to achieve a target concentration of 40 ng/g in the egg contents. Transfer efficiency was higher for BDE-47 than for BDE-99 in the red-eared sliders (25.8 ± 1.9% vs 9.9 ± 1.1%) and snapping turtles (31.3 ± 1.6% vs 12.5 ± 1.4%), resulting in greater BDE-47 and lower BDE-99 egg content concentrations relative to the 40 ng/g target. However, only 25.8 and 31.3% of the total BDE-47 and 9.9 and 12.5% of the total BDE-99 dose applied could be accounted for in the red-eared slider and snapping turtle egg contents, respectively. Additionally, increased BDE-47 in red-eared slider egg contents dosed with only BDE-99 indicate that BDE-99 might have been debrominated to BDE-47. The efficacy of topical dosing for administering desired embryonic exposures is clearly affected by the chemical properties of the applied compounds and was more successful for BDE-47 in both species. Environ. Toxicol. Chem. 2013;32:393–400. © 2012 SETAC
Time-dependent species sensitivity distributions
Authors: Fox David R. , Billoir Elise 
Published: November 2012

Abstract
Time is a central component of toxicity assessments. However, current ecotoxicological practice marginalizes time in concentration–response (C-R) modeling and species sensitivity distribution (SSD) analyses. For C-R models, time is invariably fixed, and toxicity measures are estimated from a function fitted to the data at that time. The estimated toxicity measures are used as inputs to the SSD modeling phase, which similarly avoids explicit recognition of the temporal component. The present study extends some commonly employed probability models for SSDs to derive theoretical results that characterize the time-dependent nature of hazardous concentration (HCx) values. The authors' results show that even from very simple assumptions, more complex patterns in the SSD time dependency can be revealed. Environ. Toxicol. Chem. 2013;32:378–383. © 2012 SETAC
Derivation of a benchmark for freshwater ionic strength
Authors: Cormier Susan M. , Suter Glenn W. , Zheng Lei 
Published: November 2012

Abstract
Because increased ionic strength has caused deleterious ecological changes in freshwater streams, thresholds for effects are needed to inform resource-management decisions. In particular, effluents from surface coal mining raise the ionic strength of receiving streams. The authors developed an aquatic life benchmark for specific conductance as a measure of ionic strength that is expected to prevent the local extirpation of 95% of species from neutral to alkaline waters containing a mixture of dissolved ions in which the mass of SO 42− + HCO 3− ≥ Cl−. Extirpation concentrations of specific conductance were estimated from the presence and absence of benthic invertebrate genera from 2,210 stream samples in West Virginia. The extirpation concentration is the 95th percentile of the distribution of the probability of occurrence of a genus with respect to specific conductance. In a region with a background of 116 µS/cm, the 5th percentile of the species sensitivity distribution of extirpation concentrations for 163 genera is 300 µS/cm. Because the benchmark is not protective of all genera and protects against extirpation rather than reduction in abundance, this level may not fully protect sensitive species or higher-quality, exceptional waters. Environ. Toxicol. Chem. 2013;32:263–271. © 2012 SETAC
Extraction of sediment-associated polycyclic aromatic hydrocarbons with granular activated carbon
Authors: Rakowska M.I. , Kupryianchyk D. , Grotenhuis T. , Rijnaarts H.H.M. , Koelmans A.A. 
Published: November 2012

Abstract
Addition of activated carbon (AC) to sediments has been proposed as a method to reduce ecotoxicological risks of sediment-bound contaminants. The present study explores the effectiveness of granular AC (GAC) in extracting polycyclic aromatic hydrocarbon (PAH) from highly contaminated sediments. Four candidate GAC materials were screened in terms of PAH extraction efficiency using single-step 24-h GAC extractions, with traditional 24-h Tenax extraction as a reference. Subsequently, sorption of native PAHs to the best performing GAC 1240W (0.45–1.70 mm) was studied for sediment only and for GAC–sediment mixtures at different GAC–sediment weight ratios, using 76-µm polyoxymethylene (POM) passive samplers. Granular AC sorption parameters for PAHs were determined by subtracting the contribution of PAH sorption to sediment from PAH sorption to the GAC–sediment mixture. It appears that the binding of PAHs and the effectiveness of GAC to reduce sediment porewater concentrations were highly dependent on the GAC–sediment mixing ratio and hydrophobicity of the PAH. Despite the considerable fouling of GAC by organic matter and oil, 50 to 90% of the most available PAH was extracted by the GAC during a 28-d contact time, at a dose as low as 4%, which also is a feasible dose in field-scale applications aimed at cleaning the sediment by GAC addition and removal. Environ. Toxicol. Chem. 2013;32:304–311. © 2012 SETAC
Use of a novel sediment exposure to determine the effects of triclosan on estuarine benthic communities
Authors: Ho Kay T. , Chariton Anthony A. , Portis Lisa M. , Proestou Dina , Cantwell Mark G. , Baguley Jeffrey G. , Burgess Robert M. , Simpson Stuart , Pelletier Marguerite C. , Perron Monique M. , Gunsch Claudia K. , Bik Holly M. , Katz David , Kamikawa Anthony 
Published: November 2012

Abstract
Triclosan (5-chloro-2-[2,4-dichlorophenoxy]phenol) is a relatively new, commonly used antimicrobial compound found in many personal care products. Triclosan is toxic to marine organisms at the micrograms per liter level, can photodegrade to a dioxin, can accumulate in humans, and has been found to be stable in marine sediments for over 30 years. To determine the effects of triclosan on marine benthic communities, intact sediment cores were brought into the laboratory and held under flowing seawater conditions. A 2-cm layer of triclosan-spiked sediment was applied to the surface, and after a two-week exposure the meio- and macrofaunal communities were assessed for differences in composition relative to nonspiked cores. A high triclosan treatment (180 mg/kg dry wt) affected both the meio- and the macrobenthic communities. There were no discernible differences with a low-triclosan treatment (14 mg/kg dry wt). This exposure method is effective for testing the benthic community response to sediment contaminants, but improvements should be made with regard to the amount and method of applying the overlying sediment to prevent smothering of fragile benthic organisms. Environ. Toxicol. Chem. 2013;32:384–392. © 2012 SETAC
A Roe scheme for a compressible six‐equation two‐fluid model
Authors: Morin Alexandre , Flåtten Tore , Munkejord Svend Tollak 
Published: November 2012

SUMMARYWe derive a partially analytical Roe scheme with wave limiters for the compressible six‐equation two‐fluid model. Specifically, we derive the Roe averages for the relevant variables. First, the fluxes are split into convective and pressure parts. Then, independent Roe conditions are stated for these two parts. These conditions are successively reduced while defining acceptable Roe averages. For the convective part, all the averages are analytical. For the pressure part, most of the averages are analytical, whereas the remaining averages are dependent on the thermodynamic equation of state. This gives a large flexibility to the scheme with respect to the choice of equation of state. Furthermore, this model contains nonconservative terms. They are a challenge to handle right, and it is not the object of this paper to discuss this issue. However, the Roe averages presented in this paper are fully independent from how those terms are handled, which makes this framework compatible with any treatment of nonconservative terms. Finally, we point out that the eigenspace of this model may collapse, making the Roe scheme inapplicable. This is called resonance. We propose a fix to handle this particular case. Numerical tests show that the scheme performs well. Copyright © 2012 John Wiley & Sons, Ltd.
HBx‐dependent activation of twist mediates STAT3 control of epithelium‐mesenchymal transition of liver cells
Authors: Teng Jing , Wang Xiaoqian , Xu Zhenxing , Tang Nanhong 
Published: November 2012

AbstractThis study investigated the molecular mechanisms of liver cells with HBx expression on epithelium–mesenchymal transition (EMT) change using Western blot analysis and Transwell assay to assess EMT‐related protein expression and cell mobility. Luciferase reporter assay and chromatin immunoprecipitation (ChIP) assay were used to test the Twist promoter containing different STAT3 binding loci. Electrophoretic mobility band‐shift assay (EMSA) was used to detect Twist activity. Results showed that HBx expression affected the EMT‐related protein expression and the cell mobility of liver cancer cells (MHCC97) and liver cells (HL‐7702) in vitro or in vivo. These proteins exhibited reversed expression to a certain extent after Twist inhibition. In addition, the wound‐healing capability and the mobility of HL‐7702/HBx cells were lower than those treated with control‐siRNA. The expressions of p‐STAT3 and Twist were positively correlated with HBx expression. The second STAT‐3 binding sequence in the Twist promoter region of the HL‐7702/HBx cells was the first locus. Twist activity in the HL‐7702/HBx2 cells was higher than that in HL‐7702 cells. Moreover, the activity decreased when the cells were treated with HBx‐siRNA to inhibit HBx expression, or with STAT3 inhibitor to reduce STAT3 activation. Therefore, Twist is essential for the regulation of the mobility of liver cells with HBx expression. HBx activates the Twist promoter by activating STAT3 and promotes EMT occurrence in liver cells. J. Cell. Biochem. 114: 1097–1104, 2013. © 2012 Wiley Periodicals, Inc.
TGF‐β/BMP signaling pathway is involved in cerium‐promoted osteogenic differentiation of mesenchymal stem cells
Authors: Liu Dan‐Dan , Zhang Jin‐Chao , Zhang Qun , Wang Shu‐Xiang , Yang Meng‐Su 
Published: November 2012

AbstractThe extensive applications of cerium (Ce) increased the chance of human exposure to Ce and its compounds. It was reported that Ce was mainly deposited in the bone after administration. However, the potential effect and mechanism of Ce on bone metabolism are not well understood. In this study, we investigated the cellular effects of Ce on the differentiation of mesenchymal stem cells (MSCs) and the associated molecular mechanisms. The results indicated that Ce promoted the osteogenic differentiation and inhibited the adipogenic differentiation of MSCs at cell level. Genes involved in transforming growth factor‐β/bone morphogenetic proteins (TGF‐β/BMP) signaling pathway were significantly changed when the MSCs were exposed to 0.0001 µM Ce by RT2Profiler™ PCR Array analysis. The expression of genes and proteins related to pathways, osteogenic, and adipogenic biomarkers of MSCs upon interaction with Ce was further confirmed by quantitative real‐time reverse transcriptase polymerase chain reaction (Q‐PCR) and Western blot analysis. The results suggest that Ce exerts the effects by interacting with bone morphogenetic protein receptor (BMPR) and activates TGF‐β/BMP signaling pathway, leads to the up‐regulation of the osteogenic master transcription factor, runt‐related transcription factor 2 (Runx 2), and the down‐regulation of the adipocytic master transcription factor, peroxisome proliferator‐activated receptor gamma 2 (PPARγ2). Runx2, which subsequently up‐regulates osteoblast (OB) marker genes collagen I (Col I) and BMP2 at early stages, alkaline phosphatase (ALP), and osteocalcin (OCN) at later stages of differentiation, thus driving MSCs to differentiate into OBs. The results provide novel evidence to elucidate the mechanisms of bone metabolism by Ce. J. Cell. Biochem. 114: 1105–1114, 2013. © 2012 Wiley Periodicals, Inc.
Dopamine agonists and delusional jealousy in Parkinson's disease: A cross‐sectional prevalence study
Authors: Poletti Michele , Perugi Giulio , Logi Chiara , Romano Anna , Del Dotto Paolo , Ceravolo Roberto , Rossi Giuseppe , Pepe Pasquale , Dell'Osso Liliana , Bonuccelli Ubaldo 
Published: November 2012

AbstractBackground:Delusional jealousy (DJ) has been described in patients with Parkinson's disease (PD) on dopaminergic therapy, but a role for dopaminergic therapy in DJ has not been established.Methods:The current cross‐sectional study on DJ investigated its association with dopaminergic therapies compared with their associations with hallucinations and its prevalence in PD patients. Eight hundred five consecutive patients with PD were enrolled between January 2009 and June 2010.Results:DJ was identified in 20 patients (2.48%) and hallucinations in 193 patients (23.98%). In the multivariate logistic regression analyses, dopamine agonists were significantly associated with DJ (odds ratio, 18.1; 95% CI, 3.0–infinity; P = .0002) but not with hallucinations (odds ratio, 0.73; 95% CI, 0.49–1.10; P = .133).Conclusions:These findings suggest that dopamine agonist treatment represents a risk factor for DJ in PD independent of the presence of a dementing disorder, and the presence of this additional nonmotor side effect should be investigated in this clinical population. © 2012 Movement Disorder Society
The relationship between intracellular and plasma levels of folate and metabolites in the methionine cycle: A model
Authors: Duncan Tanya M. , Reed Michael. C. , Nijhout H. Frederik 
Published: November 2012

ScopeFolate status and the status of the methionine cycle are typically assessed by measuring folate and metabolites in the plasma. It is assumed that plasma metabolite levels are proportional to their levels in tissues, but there is little information to support this assumption.Methods and resultsWe developed a mathematical model, based on known kinetics of the methionine cycle in the liver and tissues, and the transport kinetics of metabolites into and out of the plasma. We use the model to explore the relationship between folate status and metabolite values in the plasma, the relationships between metabolite values and methylation capacity, the response to a methionine load, and the half‐life of folate in plasma and tissues. We also use the model to study the effects of Down syndrome and oxidative stress on metabolite values in plasma and tissues. The model accurately reproduces measured metabolite values pre‐ and post‐folate fortification. The model shows that a high acute intake of folate remains largely restricted to the plasma and is rapidly excreted; only a prolonged exposure to increased folate elevates tissue folate significantly.ConclusionThe model accurately reproduces experimental and clinical findings and can serve as a platform to study, in silico, the relationships between metabolite values in tissues and plasma, and how these vary with methionine and B vitamin input, and with mutations in the genes for enzymes in the methionine cycle.
Determining crane areas for balancing workload among interfering and noninterfering cranes
Authors: Boysen Nils , Emde Simon , Fliedner Malte 
Published: November 2012

AbstractThis article treats the problem of subdividing an area for storing containers such that the workload is evenly shared among the cranes operating the resulting subareas. We consider two crane sets: while noncrossing constraints between cranes of the same set need to be observed, cranes of different sets do not interfere. Such a problem setting is, for instance, relevant for scheduling the (un‐)loading of vessels by parallel quay cranes operating on opposing berths or in container yards with cross‐over cranes. We formalize the resulting optimization problem, prove computational complexity, and present exact and heuristic solution procedures. © 2012 Wiley Periodicals, Inc. Naval Research Logistics, 2012
Establishing Project Portfolio Management: An Exploratory Analysis of the Influence of Internal Stakeholders’ Interactions
Authors: Beringer Claus , Jonas Daniel , Gemünden Hans Georg 
Published: November 2012

Project portfolio management (PPM) is viewed as a management innovation that must be further established and professionalized in many firms. Stakeholder behavior and stakeholder management are key success factors for project portfolios. Furthermore, stakeholder management must not only focus on single stakeholders but also account for stakeholders influencing one another in fairly complex interactions of multiple and potentially interdependent stakeholders. Our quantitative study of internal key stakeholders’ engagement in the PPM process is based on a survey using 223 project portfolios from medium to large firms in Austria, Germany, and Switzerland. We investigate the intensity of stakeholders’ engagement in the three phases of the PPM process compared with expectations from PPM standards and role descriptions. Further, we analyze the interdependencies between the levels of stakeholder engagement and identify patterns of stakeholder behavior. Our study assists in explaining the relevance of stakeholders to PPM. We further integrate stakeholder theory with a highly relevant management approach (PPM) and thus enhance the explanatory value of stakeholder theory. Finally, the current findings enable managers to address stakeholders more effectively through an increased understanding of stakeholder behavior and its consequences.
Interactive energy transfer between Cu2+ and Yb3+ in Ca1‐xCuSi4O10: Ybx
Authors: Zhuang Yixi , Tanabe Setsuhisa 
Published: November 2012

AbstractX‐ray diffraction patterns, photoluminescence, excitation spectra, and luminescence decay curves of polycrystals with compositions of Ca1‐xCuSi4O10: Ybx (x = 0.00‐0.10) were measured. The samples showed intense absorption bands at 540, 630, 800 nm due to Cu2+: 2B1g→ 2A1g, 2Eg, 2B2g transitions, and efficient photoluminescence bands at 920 nm from Cu2+ and 1007 nm from Yb3+, respectively. With increasing of Yb concentration, the intensity of Yb3+ peaks increased, while that of the Cu2+ broadband luminescence did not decrease much. Possible energy transfer mechanisms were discussed based on result of luminescence decay curves of both active ions. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
New organic‐inorganic hybrid ureasil‐based polymer and glass‐polymer composites with ion‐implanted silver nanoparticles
Authors: Kavetskyy Taras , Lyadov Nikolay , Valeev Valery , Tsmots Volodymyr , Petkova Tamara , Boev Victor , Petkov Plamen , Stepanov Andrey L. 
Published: November 2012

AbstractThe micro‐/nanoscopic structure of the hybrid organic‐inorganic materials, based on polyether chains covalently linked to a silica framework through urea bridges, referred as ureasilicates or ureasils, and As2S3‐ureasil composites with ion‐implanted Ag nanoparticles is investigated. The formation of Ag nanoparticles is confirmed using optical transmission (surface plasmon resonance band of Ag nanoparticles) at ion‐implantation doses of 2.5×1016 and 5.0×1016 ion/cm2 on the example of ureasil. It is established with scanning electron microscopy that incorporation of the As2S3 clusters into ureasil assists to ion‐synthesis of Ag nanoparticles in polymer matrix, more effectively at higher doses of ion‐implantation and for silver containing (As2S3)95Ag5‐ureasil composite. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Investigating the Relative Importance of Individual Differences on the Work–Family Interface and the Moderating Role of Boundary Preference for Segmentation
Authors: Michel Jesse S. , Clark Malissa A. 
Published: November 2012

AbstractThis study examines the relative importance of individual differences in relation to perceptions of work–family conflict and facilitation, as well as the moderating role of boundary preference for segmentation on these relationships. Relative importance analyses, based on a diverse sample of 380 employees from the USA, revealed that individual differences were consistently predictive of self‐reported work–family conflict and facilitation. Conscientiousness, neuroticism, negative affect and core self‐evaluations were consistently related to both directions of work–family conflict, whereas agreeableness predicted significant variance in family‐to‐work conflict only. Positive affect and core self‐evaluations were consistently related to both directions of work–family facilitation, whereas agreeableness and neuroticism predicted significant variance in family‐to‐work facilitation only. Collectively, individual differences explained 25–28% of the variance in work–family conflict (primarily predicted by neuroticism and negative affect) and 11–18% of the variance in work–family facilitation (primarily predicted by positive affect and core self‐evaluations). Moderated regression analyses showed that boundary preference for segmentation strengthened many of the relationships between individual differences and work–family conflict and facilitation. Implications for addressing the nature of work and family are discussed. Copyright © 2012 John Wiley & Sons, Ltd.
High‐Sensitivity Real‐Time Analysis of Nanoparticle Toxicity in Green Fluorescent Protein‐Expressing Zebrafish
Authors: Pan Yu , Leifert Annika , Graf Michael , Schiefer Frank , Thoröe‐Boveleth Sven , Broda Janine , Halloran Mary C. , Hollert Henner , Laaf Dominic , Simon Ulrich , Jahnen‐Dechent Willi 
Published: November 2012

AbstractGold nanoparticles (AuNP) show great potential for diagnostic and therapeutic application in humans. A great number of studies have tested the cytotoxicity of AuNP using cell culture. There is, however, an urgent need to test AuNP in vertebrate animal models that interrogate biodistribution and complex biological traits like organ development, whole body metabolism, and cognitive function. The sheer number of different compounds precludes the use of small rodent model for initial screening. The extended fish embryo test (FET) is used here to bridge the gap between cell culture and small animal models. A study on the toxicity of ultrasmall AuNP in wild type and transgenic zebrafish is presented. FET faithfully reproduce all important findings of a previous study in HeLa cells and add new important information on teratogenicity and hepatotoxicity that could not be gained from studying cultured cells.
Syntheses and Structures of Terpyridine‐Metal Complexes
Authors: Zhang Na , Yang Juan , Hu Rui‐Xiang , Zhang Man‐Bo 
Published: November 2012

AbstractSeven complexes, [Ln(ctpy)(NO3)2]n and M(ctpy)2·4H2O [Ln = Gd (1), Dy (2), Er (3); M = Co (4), Ni (5), Cu (6), Zn (7)] with the ligand 2, 2′:6′,2′′‐terpyridine‐4′‐carboxylic acid (Hctpy) were hydrothermally synthesized. X‐ray diffractional analysis reveals that the isomorphous compounds 1–3 adopt one‐dimensional chain‐like structures, whereas 4–7 are isomorphic monomers. Luminescence spectroscopy measurements indicates that compound 7 exhibits photoluminescence in the solid state at room temperature.