bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers and facilitators of telehealth for caregivers and older adults with dementia
Authors: Schwenger Layana Liss , Tsen Carolina , Molina Andréa Rodrigues Amorim , Neto Décio Bueno , Ansai Juliana Hotta , Andrade Larissa Pires , Silva Maria Jasmine Gomes , Porto Mariana , Gomes Grace Angelica Oliveira 
Published: July 2024

Abstract

Introduction: Telehealth programmes have become an alternative to the social isolation imposed by the pandemic. However, few studies analyze existing barriers and facilitators on programmes for caregivers and older people with dementia.
Purpose: To analyze the barriers and facilitators of a telehealth program for caregivers of older people with dementia during the COVID-19 pandemic.
Methods: This quantitative-qualitative study took place online, from 2021 to 2022. 41 caregivers and 41 older people with dementia participated in an online intervention consisting of psychoeducation and barriers management techniques, a 12 week physical exercise programme and participants meetings. Barriers and facilitators were measured in 6 time-points throughout the intervention by questionnaire. After intervention, a focus group was applied with the caregivers. Descriptive and comparative analysis between the six time-points were carried out by generalized estimating equations. Qualitative data were analyzed and categorized through Bardin's content analysis.
Results: From a total of 254 barriers, 24.4% were related to the caregiver; 49.2% to the older person and 26.4% to other aspects. The facilitators were the offered programme being online, flexible schedules, the high quality of services provided, a self-explanatory platform and the quality of the materials available. The number of barriers were higher in the first time-point compared to the fourth time point (p=0.023). Modifiable barriers (n=69) were different between the six time-points with superior values from the first to the third and fourth time-point (p=0.003).
Conclusion: This study highlights the importance of analyzing conditions and factors that can hinder or contribute to the functioning and adherence to telehealth programs. Future similar programs to be offered may benefit from the results of this investigation.
Trial Registration Number: Research Ethics Committee on Human Beings at the Federal University of São Carlos-SP (CAAE: 34696620.0.0000.5504/CEP: 6.428.315)
Study registration: Registered in the Brazilian Registry of Clinical Trials (accession number RBR-825p57). Date of Registration: 10/08/2020.
Exploring the Molecular Mechanism of Action of rhein for the Treatment of chronic kidney disease, Using Literature review, NetworkPharmacology and Molecular Docking
Authors: Wu Xiaoxiao , Peng Li , Cheng LiangHua , Wang Fan , Liu Meiyou , Wang Jingwen , Jia Yanyan 
Published: July 2024

Abstract

Purpose
To predict the possible mechanism of rhein in the treatment of chronic kidney disease (CKD) through literature review, network pharmacology and molecular docking.
Methods
Databases including PubMed, Web of Science and CNKI were searched the article about the mechanisms of rhein in treating CKD. The related targets of rhein were obtained from the Traditional Chinese Medicine Systems Pharmacology, SwissTargetPrediction and BATMAN-TCM database. And the tumor-related targets were screened out from GeneCards, Online Mendelian Inheritance in Man (OMIM) and therapeutic target database (TTD) databases. The common targets of rhein and CKD via the Venn diagram. The protein interactions network was constructed using the STRING database. The hub genes of rhein against CKD were constructed by using Cytoscape 3.6.0 software. GO and KEGG pathways involved in the targets were analyzed by using the DAVID database. Autodock Vina software was used to verify the molecular docking of rhein and 5 key targets.
Results
Literature review showed that the signaling pathways of rhein against CKD were Klotho, STAT3, BMP7, TLR4, TRK, NF-ƙB, SHH-Gli1-Snail, ROS/Akt, MMP-9/TIMP-1, PPAR–α–CPT1A, SIRT3/FOXO3a, SirT1/STAT3/Twist1, AMPK/mTOR, TGF-β1/α-SMA, lincRNA-Cox2/miR-150-5p/STAT1.In the BATMAN-TCM database, the rhein enrichment analysis pathways were PPAR, Jak-STAT, VEGF, p53, NF-kappa B, HIF-1, TNF and AMPK. The PPI network revealed that ALB, MPP9, CASP3, IL1B, PTGS2, ICAM1, AKT1, MPP2, PPARG and SERPINE1 were the ten most relevant targets. GO and KEGG analysis showed that rhein and CKD were regulating 8 important signaling pathways in 3 biological processes. According to the results of molecular docking, the rhein binding with PTGS2 showed the highest binding energy (− 9.6 kcal/mol).
Conclusions
Literature review, network pharmacology and molecular docking predicate the possible mechanisms of rhein in treating CKD, and provided a new direction for the development of new drugs for the treatment of CKD.
Long-term after-effects of mental rotation training: a behavior and ERP study
Authors: Ke Yufeng , Dong Linlin , Zhu Xiaodong , Liu Shuang , Ming Dong 
Published: July 2024

Abstract

Mental rotation is a crucial cognitive skill underlying performance in various spatial tasks. It can be enhanced through repetitive practice in previous studies. However, the long-term efficacy of training and non-invasive brain stimulation and its associated neurophysiological correlates are yet to be elucidated. This study investigated the long-term after-effects of 10 days of mental rotation training with high-definition transcranial direct current stimulation (HD-tDCS) on behavioral performance and neural electrophysiology in 34 healthy participants. Mental rotation tests along with EEG recording were performed 1 day before training, 1 day, 20 days, and 90 days after training. While no significant group differences emerged due to stimulation, training resulted in improved task accuracy, decreased response time, and increased task-evoked EEG responses. These effects diminished over time in the follow-up tests but remained superior to pre-training levels at 90 days. Surprisingly, there was still a sustained elevation in task-evoked neurophysiological responses 20 days after training compared to 1 day after training. Individual differences, such as gender and baseline performance, probably modulated training outcomes and long-term after-effects. These findings shed light on the potential of mental rotation training to enhance cognitive function and highlight the feasibility and necessity for further exploration of training to enhance cognitive-related neuroplasticity.
Low sintering, characterization of Nd 3+ substituted Ni-Mg-Cu spinel ferrites, containing structural, optical, and magnetic properties for medicinal Applications
Authors: Jyothirmai E. , Pandari J. , Prasad K.Venu , Aparna Dode , Ludhiya V. , Rani P. , Nathanial V. 
Published: July 2024

Abstract

The citric acid-assisted auto combustion procedurewas used to successfully synthesis cubic spinel ferrites comprising Ni0.25Mg0.25Cu0.5NdxFe2−xO4 (where x = 0.00, 0.03, 0.06, and 0.1). The presence of cubic spinel ferrites has been verified by X-ray diffraction. Which also showed decreasing crystallite morphologies with a range of 37 to 25 nm. The findings for the confirmation of cubic spinel ferrites are highly supported by FTIR, which shows the creation of two metallic bands. FE-SEM analysis of the morphology of produced ferrites showed aggregation and porous, spherical-like formations. The findings of the UV-vis investigation indicate a violet shift and a range of 1.48 to 1.28 eV for the estimated direct band gap energy values. All synthetic ferrite samples have a vibrating sample magnetometer, which contributes to their soft ferromagnetic nature and the reduction of super exchange interactions.
Identification of novel prognostic targets in Gastric Cancer using bioinformatics analysis
Authors: Chen Huanting , Zhang Xiaomin , Li Guoqiang , Li Xin , Yang Siran , Liu Yajie , Yang Mengqi 
Published: July 2024

Abstract

Background: Gastric cancer (GC) is a deadly malignancy with an ever-increasing incidence worldwide. The potential prognostic biomarkers and novel therapeutic targets remain to be explored. This study builds up predictive gene targets of expression alterations in GC utilizing bioinformatics analysis.
Methods and results: Themicroarray datasets (GSE19826, GSE54129 and GSE79973) associated with GC were obtained from Gene Expression Omnibus (GEO) database to identify the differentially expressed genes (DEGs) between GC and non-tumor tissues. Among all these up-regulated genes, we focused on WISP1 belong to the cellular communication network (CCN) family. We analyzed the mRNA and protein expressions of CCN family in GC utilizing GEPIA, ONCOMINE, and HPA databases. A significant correlation was found between the expression of WISP1 and the pathological stage of patients with GC. The prognostic value of distinct CCN family members in patients were analyzed using the Kaplan–Meier plotter database. Patients with low transcription levels of WISP1 were significantly associated with better prognosis. To furtherly clarify the important role of WISP1 in GC cells, we performed colony formation assay and scratch test. The experimental data showed that inhibiting the expression of WISP1 led to attenuatedcell proliferation and migration.
Conclusions: Analysis of multiple datasets combined with global network information and experimental verification provides a successful approach to uncover the potential prognostic biomarkers and therapeutic targets of GC. Our investigation identified the risk and prognostic related gene WISP1 and presented a novel perspective to improve the prognostic and therapeutic outcomes of GC.
Screening and validation of key genes associated with osteoarthritis
Authors: He MingLiu , Yu QiFan , Xiao Han , Dong HengDa , Li DaZhuang , Gu WenGuang 
Published: July 2024

Abstract

Background
Osteoarthritis is recognized as a common geriatric condition characterized by irregular chronic pain. Its prevalence is steadily increasing, posing significant challenges to global public health, while some studies indicate a trend towards younger individuals being affected. This condition severely impacts patients' quality of life.
Methods
Using the Gene Expression Omnibus (GEO) database, we downloaded datasets GSE114007, GSE169077, and GSE206848. We utilized R software to screen and confirm differentially expressed genes (DEGs) related to the development of osteoarthritis. A cross-analysis of the three datasets was conducted, with the least overlapping dataset, GSE206848, selected as the validation set. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed on the DEGs from GSE114007 and GSE169077. Weighted Gene Co-Expression Network Analysis (WGCNA) was employed to identify modules closely associated with osteoarthritis, and genes from these intersecting modules were entered into the STRING database to construct Protein-Protein Interaction Networks. The top ten genes by connectivity were identified and validated using GSE206848. Key genes were identified and preliminarily validated using Quantitative Real-Time PCR (QPCR). Subsequent validation of related genes was carried out through Western Blot (WB) analysis.
Results
Differentially expressed genes were identified from the GSE114007 and GSE169077 datasets and validated in the GSE206848 dataset, with ANGPTL4 selected as the key gene. QPCR results indicated a significant difference in ANGPTL4 expression levels between normal and osteoarthritic chondrocytes. Western Blot analysis confirmed a significant difference in ANGPTL4 protein expression between normal and osteoarthritic chondrocytes.
Conclusion
Based on the experimental findings, ANGPTL4 appears to be a potential key gene in osteoarthritis.
Comprehensive Characterization of Visceral Adipose Tissue Proteome Reveals Distinct Metabolic Adaptations and Inflammatory Signatures in Severe Obesity
Authors: Nuutila Pirjo , Dadson Prince , Honka Miikka-Juhani , Suomi Tomi , Rokka Anne , Salminen Paulina , James Peter , Elo Laura , Olkkonen Vesa M. 
Published: July 2024

Abstract

Background and objectives
The global prevalence of severe obesity is a major public health concern due to its association with increased risk of cardiometabolic complications and mortality. Abdominal visceral adipose tissue (VAT) exhibits distinct biomolecular characteristics that contribute to these pathological processes. This study aimed to comprehensively characterize the VAT proteome in severe obesity compared to non-obese controls and investigate its association with serum biomarkers reflecting metabolic health.
Methods
We conducted a cross-sectional analysis of 46 patients with severe obesity undergoing bariatric surgery and 17 healthy non-obese controls. VAT proteomics were analyzed using liquid chromatography tandem mass spectrometry (LC-MS/MS), while serum metabolites were quantified using nuclear magnetic resonance-based high throughput metabolomics.
Results
LC-MS/MS identified 69 differentially expressed proteins (FDR < 0.01), with 44 downregulated and 25 upregulated in the obese group. Downregulated proteins were associated with carbohydrate, lipid, amino acid metabolism, and energy production. Conversely, upregulated proteins were involved in inflammation, immune activation, cellular stress response, and protein turnover.
Conclusions
These findings provide critical insights into the molecular basis of VAT in obesity, potentially paving the way for targeted therapeutic strategies. Furthermore, the differentially expressed proteins may serve as promising candidates for understanding VAT's role in obesity development.
ClinicalTrials.gov registration numbers: NCT00793143 and NCT01373892.
Intraoperative pelvic rotation in total hip arthroplasty with lateral direct anterior approach
Authors: Liu Shiwei , Liu Mingyang , Song Wenjing , Wang Jun , Zhao Shibo , Yang Chen , Qi Xin 
Published: July 2024

Abstract

The position of the acetabular cup is important for the outcome of total hip arthroplasty (THA). In THA with a lateral direct anterior approach (LDAA), the pelvis can easily move back and forth on the operating table during surgery. Intraoperative assessment of pelvic rotation is an important component for successful positioning and placement of the acetabular cup in LDAA-THA. We prospectively studied 30 cases of primary THA with LDAA. We used a specially designed mechanical brace and smartphone to measure pelvic motion in the horizontal and coronal planes to improve the accuracy of acetabular cup placement. The results showed that axial rotation of the pelvis occurred in all cases, with a rotation angle of 9.1°±1.0° (range 7.4°-10.9°). The pelvis also tilted upward or downward simultaneously, but the tilt angle was relatively small and not clinically significant. There was no statistically significant correlation between pelvic rotation and patients’ height, weight, or body mass index (BMI). The study found that the pelvis was mainly tilted backward during surgery, with an average backward tilt of 9° in the horizontal plane. Pelvic motion primarily occurred when the Hohmann retractor was used to expose the acetabulum.
The Risk of Upper Gastrointestinal Bleeding and Coagulopathy in Patients with Inflammatory Bowel disease and Cirrhosis
Authors: Rajamanuri Medha , Danduboyina Anirudh , pannala sreeram , Dar Sophia , scaife Steve , Arshad Sharjeel 
Published: July 2024

Abstract

Introduction: In patients with cirrhosis, systemic inflammation disrupts hemostatic balance similarly to inflammatory bowel disease (IBD), potentially leading to a procoagulant or anticoagulant state. Despite guidelines supporting anticoagulation use in hospitalized IBD patients, evidence is weak, and there are no specific guidelines for those with cirrhosis. This study aims to explore how cirrhosis affects coagulopathy and gastrointestinal bleeding in IBD patients to inform decisions on anticoagulation therapy. Patients and Methods: Using the National Inpatient Sample database, hospitalizations with IBD and cirrhosis diagnoses from 2016 to 2020 were identified. Pregnant women, patients on chronic anticoagulation, and those with malignancies were excluded. 1:1 matching for comorbidities was performed. Primary outcome: upper GI bleed; secondary outcomes: mortality, venous thromboembolism (VTE), length of stay. Results: Among IBD patients, 5,375 had cirrhosis. After matching, both groups comprised 4,195 patients. In matched cohorts, IBD patients with cirrhosis had increased risk of upper GI bleeding (RR-3.60, p < 0.0001) and mortality (RR-2.78, p < 0.002), with no significant difference in VTE events (RR-1.70, p-0.446). Length of stay did not differ significantly. Conclusion: This study reveals a higher incidence of upper GI bleeding in IBD patients with cirrhosis, emphasizing the need for close monitoring. The efficacy of anticoagulation in preventing thromboembolic events in these patients remains uncertain due to increased bleeding risk. Further randomized trials are needed to evaluate anticoagulation effects considering varying cirrhosis severity.
High-frequency oscillatory ventilation with or without volume guarantee during neonatal transport
Authors: Belteki Gusztav , Balog Vera , Liszkay Gabor , Lantos Lajos , Jermendy Agnes 
Published: July 2024

Abstract

Objectives
To analyse and compare ventilator parameters during high-frequency oscillatory ventilation (HFOV) with or without volume guarantee (VG) in emergency neonatal transport.
Study design:
Data were downloaded with 0.5 Hz during transport, using a fabian™ HFOi ventilator over three years (n = 51), and analysed computationally.
Results
Median deviation of mean airway pressure from its target was < 1 cmH2O. During HFOV (n = 26), the pressure amplitude differed from the target by < 1 cmH2O. During HFOV-VG (n = 22), median deviation of the oscillation volume (VThf) from its target was − 0.07 mL/kg, but in some cases VThf was by > 0.38 mL/kg below target. Increasing the maximum allowed amplitude by 10 cmH2O improved the maintenance of VThf. HFOV and HFOV-VG parameters were similar, except the lower amplitude during HFOV-VG. A VThf < 2.5 mL/kg avoided hypercapnia in most cases.
Conclusions
HFOV and HFOV-VG can be safely used during neonatal transport, maintaining ventilator parameters close to their targets.
Impact of dietary zinc on stimulated zinc secretion MRI in the healthy and malignant mouse prostate
Authors: Jordan Veronica Clavijo , Martins André F. , Dao Erica , Geraki Kalotina , Chirayil Sara , Wen Xiaodong , Khalighinejad Pooyan , Parrott Daniel , Wang Xiaojing , Pagan Patricia Gonzalez , Rofsky Neil , Farquharson Michael , Sherry A. Dean 
Published: July 2024

Abstract

The long-term goal of this work is to use glucose-stimulated zinc secretion (GSZS) MRI in men undergoing scans for prostate cancer. Given that dietary choices may affect an individual’s zinc content we examined how dietary zinc impacts prostate cancer detection via GSZS MRI in healthy and TRAMP mice. Mice were fed a low, normal, and high zinc content diet. GSZS was evaluated by MRI, tissue zinc content by synchrotron radiation X-ray fluorescence (SR-XRF), and zinc bioavailability by ICP-MS of serum. Results showed zinc deficiency caused systemic and organ-level dysregulation, weight loss, and altered zinc bioavailability. Healthy mice increased serum zinc, while TRAMP mice maintained low levels. SR-XRF indicated reduced prostate zinc in zinc-deficient diets for both WT and TRAMP mice. Normalized GSZS MRI data indicated stimulated zinc efflux increased with dietary zinc in healthy mice but not in TRAMP mice. The study underscores the potential role of dietary zinc in prostate cancer detection.
Exploring Alternative Techniques for Laparoscopic Sleeve Gastrectomy with Stapling First: the In Situ Method
Authors: Jeon Dongjae , Kim Yong Jin , Chung Yoona 
Published: July 2024

Abstract

Introduction:
Laparoscopic sleeve gastrectomy (LSG) is the most frequently performed procedure in metabolic and bariatric surgery (MBS) worldwide. Despite its technical simplicity, LSG presents challenges, such as the safe division of short gastric vessels and the uniform stapling of the stomach. This study introduces a modified approach to LSG termed the 'in situ' method, and reports its outcomes.
Materials and Methods:
A retrospective analysis was conducted on data from patients who underwent LSG using the in situ method between December 2019 and January 2022. Patient demographics, perioperative profiles, early postoperative complications, and one-year postoperative weight loss outcomes were collected.
Results:
Among 1038 primary procedures, 668 cases of LSG were analyzed. The mean age was 34.57 ± 9.90 years, with a mean preoperative BMI of 37.87 ± 5.02 kg/m². Major postoperative complications occurred in 1.2% of cases, with a bleeding rate of 1.0% and a leakage rate of 0.1%. No cases of sleeve stenosis were reported. Weight loss outcome at one-year showed BMI loss of 11.08 ± 3.84 kg/m² and %TWL of 28.67 ± 7.94 %.
Conclusion:
The in situ method is a safe and feasible approach for LSG, allowing for the safe division of short gastric vessels and uniform stapling.
⮚ The in situ method consists of applying the stapler first and subsequently transecting the short gastric vessels in a retrograde fashion.
⮚ The in situ method demonstrated comparable outcomes to the conventional approach in LSG.
⮚ The in situ method allows for the safe division of the short gastric vessels and the execution of uniform stapling on both sides of the stomach with ease.
Pre- and Post-Operative Evaluation of the Changes in Anthropometric Parameters On Female Laparoscopic Sleeve Gastrectomy Patients: Short Term Follow-Up Study
Authors: ULCAY Tufan , GÖRGÜLÜ Özkan , ÖNCÜ Ruken , ERBESLER Zeynel Abidin , ÖZDEN Hüseyin , BULUT Bekir , YAĞMUR Berat , ALADAĞ Muhammed Fırat , YÜCEL Hasan Esat , UZUN Ahmet , DOĞAN Kadirhan , ARPAÇAY Burcu KAMAŞAK 
Published: July 2024

Abstract

Purpose: Obesity is accepted as one of the most serious problem in the world. Today's advanced technology, computer systems, unmanned production processes have caused people to move less than before and cause obesity. Among treatments, bariatric interventions are the most effective for weight reduction. Here, we observe the short term (2 months) changes in anthropometric parameters in females after bariatric surgery, assess the impact of weight loss on muscle strength, and measure how much fat reduction occurs in different regions of the body during the post-operative period.
Materials and Methods: In this observational follow-up prospective study, anthropometric measurements were recorded between October 2022 and March 2023 for patients who had laparoscopic sleeve gastrectomy procedure. Measurements were taken before the surgery and in the 1st and 2nd months after the surgery.
Results: Additionally, there was a significant decrease in skinfold thickness related to fat mass, while grip strengths also showed a significant increase during this short-term 8-week period post-surgery.
Conclusion: Our study is important in terms of being the first study to evaluate skinfold thicknesses comprehensively in the short term, and it is also noteworthy that it is the first study to apply the pinch strength test in addition to the hand grip strength test.