bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Integrated Nutrient Management (INM) on Growth Attributes of Strawberry (Fragaria × ananassa Duch.) cv. Winter Dawn
Authors: Yadav Harish Chandra , Singh Anand , Srivastava Akhilesh Kumar , Singh Bijendra Kumar , Vikram Balaji , Rai Ashutosh , Bakash Hari , Prajapati Paramanand 
Published: July 2024

A study entitled Impact of Integrated Nutrient Management (INM) on growth attributes of strawberry (Fragaria × ananassa Duch.) in district Banda, Uttar Pradesh was carried out at the "Green shade Net house" located in the Department of Fruit Science, College of Horticulture, Banda University of Agriculture & Technology, Banda (U.P.), during the academic years of 2022-23 and 2023-24. Eleven treatments using various combinations of N, P, K, nano urea, bio fertilizers, and organic manures were tested in a Randomised Block Design with three replicates. The experiment's primary objective was to determine the effect of nano technology on the vegetative growth traits of strawberry cv. Winter Dawn. According to the findings of 2 years study and pooled results, application of treatment T4 [75% N (Nano Urea) + 75% PK (Basal) + 250 kg VC + AZO + PSB] proved to be most effective treatment to increase growth attributes of strawberry i.e., plant height 9.09 cm, 13.61 cm and 17.78 cm at 30, 60 and 90 DAP, respectively; plant spread i.e., 12.75 cm, 21.32 cm, and 23.44 cm at 30 ,60 and 90 DAP, respectively;number of leaves i.e., 5.75, 13.81 and 17.87 at30, 60 and 90 DAP, respectively; leaf length i.e., 4.70 cm, 5.80 cm and 8.29 cm at 30, 60 and 90 DAP, respectively; leaf width i.e., 5.94 cm, 7.53 cm and 11.60 cm at 30, 60 and 90 DAP, respectively; Leaf area (cm2) i.e., 40.31 cm2, 51.93 cm2,66.38 cm2 number of runners per plant i.e., 5.27, number of crowns per plant i.e., 4.39, biomass of fresh weight (g) i.e., 147.37 g and Biomass of dry weight (g) i.e., 36.84 g. Therefore, application of 75%N (Nano Urea) + 75% PK (Basal) + 250 Kg VC + AZO + PSB] proved to be most effective treatment to increase growth attributes of strawberry and same can be recommended to the growers.
Reliability Indices
Authors: Frattini Flavio , Bovenzi Antonio , Alonso Javier , Trivedi Kishor 
Published: January 2013

AbstractModern complex systems are expected to be reliable and to provide certain levels of performance during operation. Hence definition and evaluation of reliability and performability indices become necessary. State‐space models, such as continuous time Markov chains, are often used because of their capacity of handling different failure/repair behaviors, such as imperfect coverage, correlated failures, and repair dependencies. However time‐dependent rates associated to events and particular repair strategies make computation of indices harder. In such cases, nonhomogeneous continuous time Markov chains, semi‐Markov processes, Markov regenerative processes (MRGPs), or phase‐type approximation can be used. In this article, by means of a simple example, we show how to compute the reliability of such systems with three techniques: piecewise constant approximation, phase type expansion, and simulation.
8p23.1 duplication syndrome; common, confirmed, and novel features in six further patients
Authors: Barber John C.K. , Rosenfeld Jill A. , Foulds Nicola , Laird Sophie , Bateman Mark S. , Thomas N. Simon , Baker Samantha , Maloney Viv K. , Anilkumar Arayamparambil , Smith Wendy E. , Banks Valerie , Ellingwood Sara , Kharbutli Yara , Mehta Lakshmi , Eddleman Keith A. , Marble Michael , Zambrano Regina , Crolla John A. , Lamb Allen N. 
Published: January 2013

AbstractThe 8p23.1 duplication syndrome is a relatively rare genomic condition that has been confirmed with molecular cytogenetic methods in only 11 probands and five family members. Here, we describe another prenatal and five postnatal patients with de novo 8p23.1 duplications analyzed with oligonucleotide array comparative genomic hybridization (oaCGH). Of the common features, mild or moderate developmental delays and/or learning difficulties have been found in 11/12 postnatal probands, a variable degree of mild dysmorphism in 8/12 and congenital heart disease (CHD) in 4/5 prenatal and 3/12 postnatal probands. Behavioral problems, cleft lip and/or palate, macrocephaly, and seizures were confirmed as additional features among the new patients, and novel features included neonatal respiratory distress, attention deficit hyperactivity disorder (ADHD), ocular anomalies, balance problems, hypotonia, and hydrocele. The core duplication of 3.68 Mb contains 31 genes and microRNAs of which only GATA4, TNKS, SOX7, and XKR6 are likely to be dosage sensitive genes and MIR124‐1 and MIR598 have been implicated in neurocognitive phenotypes. A combination of the duplication of GATA4, SOX7, and related genes may account for the variable penetrance of CHD. Two of the duplications were maternal and intrachromosomal in origin with maternal heterozygosity for the common inversion between the repeats in 8p23.1. These additional patients and the absence of the 8p23.1 duplications in published controls, indicate that the 8p23.1 duplication syndrome may now be considered a pathogenic copy number variation (pCNV) with an estimated population prevalence of 1 in 58,000. © 2013 Wiley Periodicals, Inc.
Nickel‐catalyzed Hydrodecyanation of Carbon–Cyano Bonds
Authors: Weidauer Maik , Someya Chika I. , Irran Elisabeth , Enthaler Stephan 
Published: January 2013

AbstractNickel‐catalyzed hydrodecyanation of aryl and alkyl cyanides with tert‐butylmagnesium chloride has been examined in detail. With a straightforward nickel complex as the precatalyst, good to excellent yields and chemoselectivity were achieved for a variety of organic cyanides.
iCutter: a direct cut‐out tool for 3D shapes
Authors: Meng Min , Fan Lubin , Liu Ligang 
Published: January 2013

ABSTRACTWe present a novel sketch‐based tool, called iCutter (short for intelligent cutter), for cutting out semantic parts of 3D shapes. When a user performs a cutting task, he only needs to draw a freehand stroke to roughly specify where cuts should be made without much attention. Then, iCutter intelligently returns the best cut that meets the user's intention and expectation. We develop a novel scheme for selecting the optimal isoline from a well‐designed scalar field induced from the input stroke, which respects the part saliency as well as the input stroke. We demonstrate various examples to illustrate the flexibility and applicability of our iCutter tool. Copyright © 2011 John Wiley & Sons, Ltd.
Using a Fragment‐Based Approach To Target Protein–Protein Interactions
Authors: Scott Duncan E. , Ehebauer Matthias T. , Pukala Tara , Marsh May , Blundell Tom L. , Venkitaraman Ashok R. , Abell Chris , Hyvönen Marko 
Published: January 2013

AbstractThe ability to identify inhibitors of protein–protein interactions represents a major challenge in modern drug discovery and in the development of tools for chemical biology. In recent years, fragment‐based approaches have emerged as a new methodology in drug discovery; however, few examples of small molecules that are active against chemotherapeutic targets have been published. Herein, we describe the fragment‐based approach of targeting the interaction between the tumour suppressor BRCA2 and the recombination enzyme RAD51; it makes use of a screening pipeline of biophysical techniques that we expect to be more generally applicable to similar targets. Disruption of this interaction in vivo is hypothesised to give rise to cellular hypersensitivity to radiation and genotoxic drugs. We have used protein engineering to create a monomeric form of RAD51 by humanising a thermostable archaeal orthologue, RadA, and used this protein for fragment screening. The initial fragment hits were thoroughly validated biophysically by isothermal titration calorimetry (ITC) and NMR techniques and observed by X‐ray crystallography to bind in a shallow surface pocket that is occupied in the native complex by the side chain of a phenylalanine from the conserved FxxA interaction motif found in BRCA2. This represents the first report of fragments or any small molecule binding at this protein–protein interaction site.
Formation and Properties of a Hybrid Organosilica with a p‐Phenylene Vinylene Polymer Partially Grafted to the Walls
Authors: Buaki‐Sogo Mireia , de Miguel Maykel , Atienzar Pedro , Álvaro Mercedes , García Hermenegildo 
Published: January 2013

AbstractThe present manuscript reports a mesoporous organosilica (mpSiO2) containing a p‐phenylene vinylene (PPV) co‐polymer partially grafted to the walls of the hybrid material (PPV${ \subset }$mpSiO2). This material was obtained by using a bis‐silylated 2,5‐bis(chloromethylphenylene) as the silicon precursor in combination with tetraethyl orthosilicate (TEOS) and cetyltrimethylammonium bromide (CTABr) as the surfactant. The final polymer was formed by adding appropriate amounts of 2,2′‐{[2,5‐bis(chloromethyl)‐1,4‐phenylene]bis(oxy)}diethanol as the co‐monomer and KtBuO as the base. The resulting PPV${ \subset }$mpSiO2 was characterized by XRD, SEM, TEM, FTIR spectroscopy, and magic angle spinning 29Si NMR spectroscopy; all spectroscopic data were in agreement with the presence of a conducting polymer. The resulting PPV${ \subset }$mpSiO2 material exhibits electrical conductivity, particularly after I2 doping, an electrochemical response, and electroluminescence. Laser flash photolysis studies of PPV${ \subset }$mpSiO2 indicate that this material can form PPV.+ polarons that could be responsible for the electrochemical and electroluminescent response.
Electrocatalytic Hydrogenation and Deoxygenation of Glucose on Solid Metal Electrodes
Authors: Kwon Youngkook , Koper Marc T. M. 
Published: January 2013

AbstractThis Full Paper addresses the electrocatalytic hydrogenation of glucose to sorbitol or 2‐deoxysorbitol on solid metal electrodes in neutral media. Combining voltammetry and online product analysis with high‐performance liquid chromatography (HPLC), provides both qualitative and quantitative information regarding the reaction products as a function of potential. Three groups of catalysts clearly show affinities toward: (1) hydrogen formation [on early transition metals (Ti, V, Cr, Mn, Zr, Nb, Mo, Hf, Ta, We, and Re) and platinum group metals (Ru, Rh, Ir, and Pt)], (2) sorbitol formation [on late transition metals (Fe, Co, Ni, Cu, Pd, Au, and Ag) and Al (sp metal)], and (3) sorbitol and 2‐deoxysorbitol formation [on post‐transition metals (In, Sn, Sb, Pb, and Bi), as well as Zn and Cd (d metals)]. Ni shows the lowest overpotential for the onset of sorbitol formation (−0.25 V) whereas Pb generates sorbitol with the highest yield (<0.7 mM cm−2). Different from a smooth Pt electrode, a large‐surface‐area Pt/C electrode hydrogenates glucose to sorbitol from −0.21 V with relatively low current. This emphasizes the importance of the active sites and the surface area of the catalyst. The mechanism to form 2‐deoxysorbitol from glucose and/or fructose is discussed according to the observed reaction products. The yield and selectivity of hydrogenated products are highly sensitive to the chemical nature and state of the catalyst surface.
A liquid‐phase sensor using shear horizontal surface acoustic wave devices
Authors: Kondoh Jun 
Published: January 2013

AbstractA surface acoustic wave (SAW) is perturbed due to physical and/or chemical changes in an adjacent medium. If a liquid‐phase sensor is to be realized, a shear horizontal SAW (SH‐SAW) must be used. SH‐SAW sensors have been investigated since 1987. Objects measured using an SH‐SAW sensor include not only immunoreactions but also liquid properties. The advantage of an SH‐SAW sensor is the simultaneous detection of the mechanical and electrical properties of liquids. In this paper, first, the theory behind the SH‐SAW sensor is described. Then experimental results using the SH‐SAW sensor on 36YX‐LiTaO3 are introduced. In addition, the sensor sensitivities of the SH‐SAW sensors that have been fabricated on piezoelectric substrates are theoretically compared. © 2013 Wiley Periodicals, Inc. Electron Comm Jpn, 96(2): 41–49, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/ecj.10407
Tissue accumulation and species-specific metabolism of technical pentabrominated diphenyl ether (DE-71) in two predator fish
Authors: Zeng Yan-Hong , Yu Le-Huan , Luo Xiao-Jun , Chen She-Jun , Wu Jiang-Ping , Mai Bi-Xian 
Published: January 2013

Abstract
The tissue-specific accumulation and species-specific metabolism of polybrominated diphenyl ethers (PBDEs) in two predator fish species (redtail catfish and oscar fish) feeding on the same prey (tiger barb) that was exposed to technical pentabrominated diphenyl ether (DE-71) in the laboratory were investigated. The trends in the wet-weight tissue concentration of PBDEs in two predatory fish species suggested that the tissue distribution of PBDEs occurs through a series of events involving passive diffusion to the lipid compartment. A comparison of the fugacities of PBDEs in various tissues and in the serum revealed that the liver, gill, and perivisceral adipose tissue readily achieved equilibrium with the serum, but the muscle, kidney, and intestine exhibited the potential to accumulate PBDEs. The lower fugacities of PBDEs in the intestine may have significance in the transportation of PBDEs from prey to predatory fish. No tissue-specific differences in PBDE congener profiles were found, while interspecies differences in PBDE profiles were evident. The difference in profiles between two species could be attributed to species-specific debromination of PBDE. No metabolic debromination of PBDE was observed in redtail catfish, but extensive debromination of PBDEs occurred in oscar fish. Several hydroxylated PBDEs (OH-PBDEs) were detected in serum samples from the two fish species, but no methoxylated PBDEs were found. The similarities in the OH-PBDE congener profile and the ratio of OH-PBDEs to total PBDEs between the two fish species indicated that the hydroxylation of PBDEs might not be species-specific. Environ. Toxicol. Chem. 2013;32:757–763. © 2013 SETAC
The molecular profile of adult T‐cell acute lymphoblastic leukemia: Mutations in RUNX1 and DNMT3A are associated with poor prognosis in T‐ALL
Authors: Grossmann Vera , Haferlach Claudia , Weissmann Sandra , Roller Andreas , Schindela Sonja , Poetzinger Franziska , Stadler Kathrin , Bellos Frauke , Kern Wolfgang , Haferlach Torsten , Schnittger Susanne , Kohlmann Alexander 
Published: January 2013

AbstractT‐cell acute lymphoblastic leukemia (T‐ALL) is an aggressive and heterogeneous disease. The diagnosis is predominantly based on immunophenotyping. In addition to known cytogenetic abnormalities molecular mutations were recently identified. Here, 90 adult T‐ALL cases were investigated for mutations in NOTCH1, FBXW7, PHF6, CDKN2A, DNMT3A, FLT3, PTEN, and RUNX1 using 454 next‐generation amplicon sequencing and melting curve analyses. These data were further complemented by FISH, chromosome banding, array CGH, and CDKN2B promoter methylation analyses. NOTCH1 was the most frequently mutated gene with a 71.1% frequency followed by FBXW7 (18.9%), PHF6 (39.5%), DNMT3A (17.8%), RUNX1 (15.5%), PTEN (10.0%), CDKN2A (4.4%), FLT3‐ITD (2.2%), and FLT3‐TKD (1.1%). In total, 84/90 (93.3%) cases harbored at least one mutation. Combining these data with CDKN2A/B deletions and CDKN2B methylation status, we detected minimum one aberration in 89/90 (98.9%) patients. Survival analyses revealed the subtype as defined by the immunophenotype as the strongest independent prognostic factor. When restricting the survival analysis to the early T‐ALL subtype, a strong association of RUNX1 (P = 0.027) and DNMT3A (P = 0.005) mutations with shorter overall survival was observed. In conclusion, RUNX1 and DNMT3A are frequently mutated in T‐ALL and are associated with poor prognosis in early T‐ALL. © 2013 Wiley Periodicals, Inc.
Freedom of Expression and the Verification of Identity Policy in Korea
Authors: Choi Yu Jung , Choi Setbyol , Lee Myoung‐Jin 
Published: January 2013

AbstractThis study investigates the Korean Government's Verification of Identity Policy by looking at the discourse surrounding its introduction and using a survey to determine the extent to which the policy was a success. While the Internet allows unprecedented freedom of expression and public opinion gathering, it also facilitates anonymity and facelessness, which increases the possibility of damage caused by deviant online behavior. The Verification of Identity Policy was aimed at fostering a more responsible Internet culture. At the beginning, it faced fierce resistance from civic groups but media coverage of innocent and high‐profile victims has changed public opinion and facilitated the implementation of the policy in 2006. Although arguments about freedom of anonymous expression and relief of damage by restrictions on freedom continue, the Verification of Identity Policy is evaluated here as a partial success, having maintained public support despite the possibility of restrictions on freedom of expression. Nevertheless, this policy perspective recommends that the Korean Government should invest in a broader and long‐term Internet usage plan than the development of just one universal policy.
Reassessing the type I dehydroquinate dehydratase catalytic triad: Kinetic and structural studies of Glu86 mutants
Authors: Light Samuel H. , Anderson Wayne F. , Lavie Arnon 
Published: January 2013

AbstractDehydroquinate dehydratase (DHQD) catalyzes the third reaction in the biosynthetic shikimate pathway. Type I DHQDs are members of the greater aldolase superfamily, a group of enzymes that contain an active site lysine that forms a Schiff base intermediate. Three residues (Glu86, His143, and Lys170 in the Salmonella enterica DHQD) have previously been proposed to form a triad vital for catalysis. While the roles of Lys170 and His143 are well defined—Lys170 forms the Schiff base with the substrate and His143 shuttles protons in multiple steps in the reaction—the role of Glu86 remains poorly characterized. To probe Glu86′s role, Glu86 mutants were generated and subjected to biochemical and structural study. The studies presented here demonstrate that mutant enzymes retain catalytic proficiency, calling into question the previously attributed role of Glu86 in catalysis and suggesting that His143 and Lys170 function as a catalytic dyad. Structures of the Glu86Ala (E86A) mutant in complex with covalently bound reaction intermediate reveal a conformational change of the His143 side chain. This indicates a predominant steric role for Glu86, to maintain the His143 side chain in position consistent with catalysis. The structures also explain why the E86A mutant is optimally active at more acidic conditions than the wild‐type enzyme. In addition, a complex with the reaction product reveals a novel, likely nonproductive, binding mode that suggests a mechanism of competitive product inhibition and a potential strategy for the design of therapeutics.
Metal binding sites of human H‐chain ferritin and iron transport mechanism to the ferroxidase sites: A molecular dynamics simulation study
Authors: Laghaei Rozita , Evans Deborah G. , Coalson Rob D. 
Published: January 2013

AbstractWe study via all atom classical molecular dynamics (MD) simulation the process of uptake of ferrous ions (Fe2+) into the human ferritin protein and the catalytic ferroxidase sites via pores (“channels”) in the interior of the protein. We observe that the three‐fold hydrophilic channels serve as the main entrance pathway for the Fe2+ ions. The binding sites along the ion pathway are investigated. Two strong binding sites, at the Asp131 and Glu134 residues and two weak binding sites, at the His118 and Cys130 are observed inside the three‐fold channel. We also identify an explicit pathway for an ion exiting the channel into the central core of the protein as it moves to the ferroxidase site. The diffusion of an Fe2+ ion from the inner opening of the channel to a ferroxidase site located in the interior region of the protein coat is assisted by Thr135, His136 and Tyr137. The Fe2+ ion binds preferentially to site A of the ferroxidase site. © 2013 Wiley Periodicals, Inc.
Structure and function of an acetyl xylan esterase (Est2A) from the rumen bacterium Butyrivibrio proteoclasticus
Authors: Till Marisa , Goldstone David C. , Attwood Graeme T. , Moon Christina D. , Kelly Willam J. , Arcus Vickery L. 
Published: January 2013

AbstractButyrivibrio proteoclasticus is a significant component of the microbial population of the rumen of dairy cattle. It is a xylan‐degrading organism whose genome encodes a large number of open reading frames annotated as fiber‐degrading enzymes. We have determined the three‐dimensional structure of Est2A, an acetyl xylan esterase from B. proteoclasticus, at 2.1 Å resolution, along with the structure of an inactive mutant (H351A) at 2.0 Å resolution. The structure reveals two domains—a C‐terminal SGNH domain and an N‐terminal jelly‐roll domain typical of CE2 family structures. The structures are accompanied by experimentally determined enzymatic parameters against two model substrates, para‐nitrophenyl acetate and para‐nitrophenyl butyrate. The suite of fiber‐degrading enzymes produced by B. proteoclasticus provides a rich source of new enzymes of potential use in industrial settings. Proteins 2013. © 2012 Wiley Periodicals, Inc.
Adsorption and folding dynamics of MPER of HIV‐1 gp41 in the presence of dpc micelle
Authors: Hartono Yossa Dwi , Mun Yip Yew , Zhang Dawei 
Published: January 2013

AbstractMembrane‐proximal ectodomain region (MPER) of HIV‐1 gp41 is known to have several epitopes of monoclonal antibodies. It also plays an important role in the membrane fusion process that is well‐evidenced, though not well‐elucidated. There are also disputes over the true structure of MPER. In this study, MPER NMR structure in the presence of dodecylphosphatidylcholine micelle is used in the molecular dynamic simulation to elucidate structural dynamics and adsorption to model MPER interaction in a membrane environment. Polarized protein‐specific charge derived from its NMR structure is found to better preserve the helical structure found in the NMR structure compared to AMBER03 calculation. The preserved helical structure also adsorb to the micelle using the hydrophobic side‐chains, consistent to the NMR structure. Ab initio folding of MPER predicts a structure quite in well agreement with the NMR structure (RMSd 3.9 Å) and shows that the micelle plays a role in the folding process. Proteins 2013; © 2012 Wiley Periodicals, Inc.
Photosensitizer‐Incorporated Quadruplex DNA‐Gated Nanovechicles for Light‐Triggered, Targeted Dual Drug Delivery to Cancer Cells
Authors: Chen Cuie , Zhou Li , Geng Jie , Ren Jinsong , Qu Xiaogang 
Published: January 2013

AbstractA novel light‐operated vehicle for targeted intracellular drug delivery is constructed using photosensitizer‐incorporated G‐quadruplex DNA‐capped mesoporous silica nanoparticles. Upon light irradiation, the photosensitizer generates ROS, causing the DNA capping to be cleaved and allowing cargo to be released. Importantly, this platform makes it possible to develop a drug‐carrier system for the synergistic combination of chemotherapy and PDT for cancer treatment with spatial/temporal control. Furthermore, the introducing of targeting ligands further improves tumor targeting efficiency. The excellent biocompatibility, cell‐specific intracellular drug delivery, and cellular uptake properties set up the basis for future biomedical application that require in vivo controlled, targeted drug delivery.