bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of tramadol on bowel function: A randomised, placebo‐controlled trial
Authors: Larsen Isabelle M. , Okdahl Tina , Mark Esben Bolvig , Frøkjær Jens Brøndum , Drewes Asbjørn Mohr 
Published: August 2024

AbstractTramadol is a weak opioid used to treat moderate pain. Stronger opioids inhibit gastrointestinal function, but little is known about the gastrointestinal effects of tramadol. Our aim was to investigate if tramadol causes opioid‐induced bowel dysfunction (OIBD). Twenty healthy male participants (mean age 24 [range 20–31] years) were included. Tramadol (extended‐release formulation, 200 mg/day) or placebo was administered for 10 days in two study periods separated by 3 weeks. Gastrointestinal transit times and segmental volume, motility and water content were investigated with the 3D‐transit system and magnetic resonance imaging. Bowel movements and gastrointestinal symptoms were recorded daily. Tramadol prolonged colonic transit time (34 h vs. 25 h, p < 0.001) and increased small bowel motility (p < 0.01) and water content (p = 0.002) compared to placebo. Across all days of treatment, tramadol reduced the number of mean daily bowel movements (p = 0.001) and increased mean stool consistency (p = 0.006). Gastrointestinal symptom scores increased with tramadol (indigestion: +358%, p = 0.01; constipation: +475%, p = 0.01). Additionally, more participants fulfilled the diagnostic criteria for constipation after tramadol treatment compared to placebo (40% vs. 0%, p < 0.001). This study showed that tramadol treatment is associated with OIBD, and management of constipation and other bowel symptoms should, therefore, be prioritised when treating pain patients with tramadol.
Egg coverings in insects: ecological adaptation to abiotic and biotic selective pressures
Authors: Li Tian‐Hao , Wang Xingeng , Desneux Nicolas , Wang Su , Zang Lian‐Sheng 
Published: August 2024

ABSTRACTInsects have evolved a spectrum of strategies that facilitate survival in the face of adverse environmental conditions and bottom‐up or top‐down pressures. The egg is the first stage in the life cycle of most insects. It is not only immobile but in many insects is the stage that survives unfavourable seasons when food resources are unavailable. Eggs are targeted by oophagous natural enemies and also are subject to abiotic stresses. In response to these diverse stresses, insects have developed various egg protection strategies. Females of many insects lay eggs in clusters and then use their own body resources to cover them to provide protection from harsh environments and biotic attack. Such egg protection strategies have allowed some herbivorous insects to thrive in new environments and become serious invasive pests. Females of many insects protect their eggs in other ways (e.g. laying eggs in concealed places, direct parental care) while others do not provide protection at all. Here, we review various egg protective strategies in insects. Our focus is on adaptive ecological mechanisms and temporal variation as well as the benefits and costs of egg coverings. We highlight several case studies on how these egg protective traits might impede biological control of globally important agricultural and forest pests and propose a framework for incorporating egg protective traits into biological control programs especially for invasive insect pests.
Cervical mucus can be used for metabolite screening in cervical cancer
Authors: Kawasaki Rie , Kukimoto Iwao , Tsukamoto Tetsuya , Nishio Eiji , Iwata Aya , Fujii Takuma 
Published: August 2024

AbstractApproximately 660,000 women are diagnosed with cervical cancer annually. Current screening options such as cytology or human papillomavirus testing have limitations, creating a need to identify more effective ancillary biomarkers for triage. Here, we evaluated whether metabolomic analysis of cervical mucus metabolism could be used to identify biomarkers of cervical intraepithelial neoplasia (CIN) and cervical cancer. The case–control group consisted of 181 CIN, 69 squamous cell carcinoma (SCC) patients, and 48 healthy controls in the primary cohort. We undertook metabolomic analyses using ultra‐HPLC–tandem mass spectrometry. Univariate and multivariate analyses were carried out to profile metabolite characteristics, and receiver operating characteristic (ROC) analysis identified biomarker candidates. Five metabolites conferred the highest discriminatory power for SCC: oxidized glutathione (GSSG) (area under the ROC curve, 0.924; 95% confidence interval, 0.877–0.971), malic acid (0.914, 0.859–0.968), kynurenine (0.884, 0.823–0.945), GSSG/glutathione (GSH) (0.936, 0.892–0.979), and kynurenine/tryptophan (0.909, 0.856–0.961). Malic acid was the best marker for detection of CIN2 or worse (0.858, 0.793–0.922) and was a clinically useful metabolite. We confirmed the reproducibility of the results by validation cohort. Additionally, metabolomic analyses revealed eight pathways strongly associated with cervical neoplasia. Of these, only the tricarboxylic acid cycle was strongly associated with all CINs and cancer, indicating active energy production. Aberrant arginine metabolism by decreasing arginine and increasing citrulline might reduce tumor immunity. Changes in cysteine‐methionine and GSH pathways might drive the initiation and progression of cervical cancer. These results suggest that metabolic analysis can identify ancillary biomarkers and could improve our understanding of the pathophysiological mechanisms underlying cervical neoplasia.
SCYL2‐related autosomal recessive neurodevelopmental disorders: Arthrogryposis multiplex congenita‐4 and beyond?
Authors: Malbos Marlène , Vera Gabriella , Sheth Harsh , Schnur Rhonda E. , Juven Aurélien , Brehin Anne‐Claire , Sheth Jayesh , Gandhi Ajit , Shapiro Faye L. , Bruel Ange‐Line , Marguet Florent , Begtrup Amber , Monaghan Kristin G. , Safraou Hana , Brasseur‐Daudruy Marie , Mau‐Them Frédéric Tran , Duffourd Yannis , Faivre Laurence , Thauvin‐Robinet Christel , Benke Paul J. , Philippe Christophe 
Published: August 2024

AbstractSCY1‐like protein 2 (SCYL2) is a member of the SCY1‐like pseudokinase family which regulates secretory protein trafficking. It plays a crucial role in the nervous system by suppressing excitotoxicity in the developing brain. Scyl2 knockout mice have excess prenatal mortality and survivors show severe neurological dysfunction. Bi‐allelic loss‐of‐function (LOF) variants in SCYL2 were recently associated with arthrogryposis multiplex congenita‐4 (AMC4) following the report of 6 individuals from two consanguineous unrelated families. The AMC4 phenotype described included severe arthrogryposis, corpus callosum agenesis, epilepsy and frequently, early death. We describe here two additional similarly affected individuals with AMC4, including one diagnosed in the prenatal period, with bi‐allelic LOF variants in SCYL2, and two individuals homozygous for missense variants in the protein kinase domain of SCYL2 and presenting with developmental delay only. Our study confirms the association of SCYL2 with AMC4 and suggests a milder phenotype can occur, extending the phenotypic spectrum of autosomal recessive SCYL2‐related disorders.
Integrating adult occurrence and reproduction data to identify conservation measures for amphibians
Authors: Lo Parrino Elia , Ficetola Gentile Francesco , Devin Michel , Manenti Raoul , Falaschi Mattia 
Published: August 2024

AbstractMonitoring programs are pivotal to establishing sound management. Due to economic, logistic, and time limitations, monitoring programs often overlook differences among life‐history stages. However, species occurrence does not necessarily mean population viability, and it is unclear to what extent monitoring programs that do not consider separately adult presence and reproduction provide effective management indications. Unfortunately, collecting data on certain life stages requires high sampling effort, leading to a trade‐off between model reliability and resources needed for monitoring. We collected data on presence and reproduction of amphibians by monitoring 207 waterbodies in Lombardy (northern Italy) in 2017–2022. We then used multistate occupancy models to test whether certain environmental features, namely, pond area, hydroperiod, forest cover, shade, aquatic vegetation, and predators’ presence, differentially affected adult occurrence and breeding probabilities of multiple amphibian species. To assess optimal sampling efforts, we modeled the detection probabilities of adults and reproduction across multiple species. Finally, we identified the optimal monitoring strategy under different scenarios of resource availability, comparing adult‐only monitoring versus joint assessment of the occurrence of adults and reproduction. In many cases, the main drivers of adult occurrence and reproduction did not coincide because most investigated ecological variables affected one life stage or the other. Forest area, for instance, increased occurrence probabilities of adults of the endemic Rana latastei but showed no effect on their reproduction probabilities. Quantitative estimates of the sampling effort showed that occurrence of adults was easier to spot in 4 out of 7 species. Multicriteria decision analyses showed that when resources were scarce, monitoring adults was the optimal strategy for those 4 species. Conversely, with more resources, monitoring both adults and reproduction emerged as the best strategy for all the considered species. Integrated monitoring of adults and reproduction is essential to comprehensively identify effective conservation measures for amphibians.
Capacity and capability of remote sensing to inform invasive plant species management in the Pacific Islands region
Authors: Chan Carrol M. H. , Owers Christopher J. , Fuller Sascha , Hayward Matt W. , Moverley David , Griffin Andrea S. 
Published: August 2024

AbstractThe Pacific Islands region is home to several of the world's biodiversity hotspots, yet its unique flora and fauna are under threat because of biological invasions. These invasions are likely to proliferate as human activity increases and large‐scale natural disturbances unfold, exacerbated by climate change. Remote sensing data and techniques provide a feasible method to map and monitor invasive plant species and inform invasive plant species management across the Pacific Islands region. We used case studies taken from literature retrieved from Google Scholar, 3 regional agencies’ digital libraries, and 2 online catalogs on invasive plant species management to examine the uptake and challenges faced in the implementation of remote sensing technology in the Pacific region. We synthesized remote sensing techniques and outlined their potential to detect and map invasive plant species based on species phenology, structural characteristics, and image texture algorithms. The application of remote sensing methods to detect invasive plant species was heavily reliant on species ecology, extent of invasion, and available geospatial and remotely sensed image data. However, current mechanisms that support invasive plant species management, including policy frameworks and geospatial data infrastructure, operated in isolation, leading to duplication of efforts and creating unsustainable solutions for the region. For remote sensing to support invasive plant species management in the region, key stakeholders including conservation managers, researchers, and practitioners; funding agencies; and regional organizations must invest, where possible, in the broader geospatial and environmental sector, integrate, and streamline policies and improve capacity and technology access.
Global treatment of haemorrhoids—A worldwide snapshot audit conducted by the International Society of University Colon and Rectal Surgeons
Authors: Samalavicius Narimantas E. , Gupta Rakesh Kumar , Nunoo‐Mensah Joseph , Fortunato Richard , Lohsiriwat Varut , Khanal Bhawani , Kumar Abhijeet , Sah Bikash , Cerkauskaite Dovile , Dulskas Audrius ,   2023 International Society of Universities of Colon and Rectal Surgeons (ISUCRS) Collaborating Group
Published: August 2024

AbstractAimThere is no universally accepted treatment consensus for haemorrhoids, and thus, management has been individualized all over the world. This study was conducted to assess a global view of how surgeons manage haemorrhoids.MethodsThe research panel of the International Society of University Colon and Rectal Surgeons (ISUCRS) developed a voluntary, anonymous questionnaire evaluating surgeons' experience, volume and treatment approaches to haemorrhoids. The 44 multiple‐choice questionnaire was available for one month via the ISUCRS email database and the social media platforms Viber and WhatsApp.ResultsThe survey was completed by 1005 surgeons from 103 countries; 931 (92.6%) were in active practice, 819 (81.5%) were between 30 and 60 years of age, and 822 (81.8%) were male. Detailed patient history (92.9%), perineal inspection (91.2%), and digital rectal examination (91.1%) were the most common assessment methods. For internal haemorrhoids, 924 (91.9%) of participants graded them I–IV, with the degree of haemorrhoids being the most important factor considered to determine the treatment approach (76.3%). The most common nonprocedural/conservative treatment consisted of increased daily fibre intake (86.9%), increased water intake (82.7%), and normalization of bowel habits/toilet training (74.4%). Conservative treatment was the first‐line treatment for symptomatic first (92.5%), second (72.4%) and third (47.3%) degree haemorrhoids; however, surgery was the first‐line treatment for symptomatic fourth degree haemorrhoids (77.6%). Rubber band ligation was the second‐line treatment in first (50.7%) and second (47.2%) degree haemorrhoids, whereas surgery was the second‐line treatment in third (82.9%) and fourth (16.7%) degree symptomatic haemorrhoids. Rubber band ligation was performed in the office by 645(64.2%) of the participants. The most common surgical procedure performed for haemorrhoids was an excisional haemorrhoidectomy for both internal (87.1%) and external (89.7%) haemorrhoids – with 716 (71.2%) of participants removing 1, 2 or 3 sectors as necessary.ConclusionAlthough there is no global haemorrhoidal treatment consensus, there are many practice similarities among the different cultures, resources, volume and experience of surgeons around the world. With additional studies, a consensus statement could potentially be developed.
Small molecule valproic acid enhances ventral patterning of human neural tube organoids by regulating Wnt and Shh signalling
Authors: Zheng Yuanyuan , Zhang Fangrong , Nie Haifeng , Li Xinyu , Xun Jiali , Fu Jianping , Wu Lijun 
Published: August 2024

AbstractValproic acid (VPA), a clinically approved small molecule, has been reported to activate Wnt signalling that is critical for dorsal–ventral (DV) patterning of neural tube. However, little is known about the impact of VPA on DV patterning process. Here, we show that even though VPA has a negative impact on the early formation of human neural tube organoids (hNTOs), it significantly enhances the efficiency of ventrally patterned hNTOs, when VPA is added during the entire differentiation process. RNA sequencing and RT‐qPCR analysis demonstrates VPA activates endogenous Wnt signalling in hNTOs. Surprisingly, transcriptome analysis also identifies upregulation of genes for degradation of GLI2 and GLI3 proteins, whose truncated fragment are transcriptional repressors of Shh signalling. The Western‐blot analysis confirms the increase of GLI3R proteins after VPA treatment. Thus, VPA might enhance ventral patterning of hNTOs through both activating Wnt, which can antagonise Shh signalling by inducing GLI3 expression, and/or inhibiting Shh signalling by inducing GLI protein degradation. We further obtain results to show that VPA still increases patterning efficiency of hNTOs with a weak influence on their early formation when the initiation time of VPA is delayed and its duration is reduced. Taken together, this study demonstrates that VPA enhances the generation of more reproducible hNTOs with ventral patterning, opening the avenues for the applications of hNTOs in developmental biology and regenerative medicine.
Graft Steatosis and Donor Diabetes Mellitus Additively Impact on Recipient Outcomes After Liver Transplantation—A European Registry Study
Authors: Sonneveld Milan J. , Parouei Fatemeh , den Hoed Caroline , de Jonge Jeroen , Salarzaei Morteza , Porte Robert J. , Janssen Harry L. A. , de Rosner‐van Rosmalen Marieke , Vogelaar Serge , van der Meer Adriaan J. , Maan Raoel , Murad Sarwa Darwish , Polak Wojciech G. , Brouwer Willem Pieter 
Published: August 2024

ABSTRACTBackground and AimsBiopsy‐proven severe graft steatosis is associated with adverse outcomes after liver transplantation. The concomitant presence of metabolic risk factors might further increase this risk. We studied the association between graft steatosis and metabolic risk factors in the donor, with recipient outcomes after liver transplantation.MethodsWe analyzed data from all consecutive first adult full‐graft donation after brain death (DBD) liver transplantations performed in the Eurotransplant region between 2010 and 2020. The presence of graft steatosis and metabolic risk factors was assessed through a review of donor (imaging) reports, and associations with recipient retransplantation‐free survival were studied through survival analyses.ResultsOf 12 174 transplantations, graft steatosis was detected in 2689 (22.1%), and donor diabetes mellitus (DM), hypertension, and dyslipidemia were present in 1245 (10.2%), 5056 (41.5%), and 524 (4.3%). In multivariable Cox regression analysis, graft steatosis (adjusted HR [aHR] 1.197, p < 0.001) and donor DM (aHR 1.157, p = 0.004) were independently associated with impaired retransplantation‐free survival. Graft steatosis and donor DM conferred an additive risk of retransplantation or death (DM alone, aHR: 1.156 [p = 0.0185]; steatosis alone, aHR: 1.200 [p < 0.001]; both steatosis and DM, aHR: 1.381 [p < 0.001]). Findings were consistent in sensitivity analyses focusing on retransplantation‐free survival within 7 days.ConclusionsGraft steatosis and donor diabetes mellitus additively increase the risk of retransplantation or death in adult DBD liver transplantation. Future studies should focus on methods to assess and improve the quality of these high‐risk grafts. Until such time, caution should be exercised when considering these grafts for transplantation.
Personhood: An emergent view from Africa and the West
Authors: Jecker Nancy S. , Atuire Caesar A. 
Published: August 2024

AbstractAfrican understandings of personhood are complex, with different accounts emphasizing distinct aspects of what it means to be a person. Some accounts stress excellence of character and performing well in social roles and relationships, while others focus on innate moral qualities of individuals independent of their conduct and character. This paper sheds new light on these twin aspects of personhood. It proposes a way to navigate these dual features by bringing African and Western personhood into conversation, building on the strengths of each approach, and developing a new view of personhood that we call, Emergent Personhood. Section 1 introduces diverse approaches to personhood within African thought. Section 2 compares African and Western approaches. Section 3 evaluates advantages and disadvantages of each and identifies conditions that any account of personhood must meet to leverage the advantages and avoid the disadvantages identified. Section 4 introduces Emergent Personhood, which meets these conditions. Section 5 concludes that expanding the conversation about personhood across cultures enriches an ongoing conversation about what it means to be a person.
Degree of joint risk factor control and incident chronic kidney disease among individuals with obesity
Authors: Tang Rui , Kou Minghao , Li Xiang , Wang Xuan , Ma Hao , Heianza Yoriko , Qi Lu 
Published: August 2024

AbstractAimTo investigate the extent to which joint risk factor control might attenuate the excess risk of chronic kidney disease (CKD) in participants with obesity.Patients and MethodsWe included a total of 97 538 participants who were obese at baseline and matched 97 538 normal weight control participants from the UK Biobank in the analysis. The degree of joint risk factor control was assessed based on six major CKD risk factors, including blood pressure, glycated haemoglobin, low‐density lipoprotein cholesterol, albuminuria, smoking and physical activity. The Cox proportional hazards models were used to estimate associations between the degree of risk factor control and risk of CKD, following participants from their baseline assessment until the occurrence of CKD, death, or the end of the follow‐up period.ResultsAmong participants with obesity, joint risk factor control showed an association with a stepwise reduction of incident CKD risk. Each additional risk factor control corresponded to an 11% (hazard ratio: 0.89; 95% confidence interval: 0.86–0.91) reduced risk of CKD among participants with obesity, with the optimal controlling of all six risk factors associated with a 49% (hazard ratio: 0.51; 95% confidence interval: 0.43–0.61) decrease in risk of CKD. Furthermore, in individuals with obesity who jointly controlled all six risk factors, the excess risk of CKD associated with obesity was effectively neutralized compared with normal weight control subjects. Notably, the protective correlations between the degree of joint risk factor control and the incidence of CKD were more pronounced in men compared with women, in individuals with a lower healthy food score versus a higher score, and among diabetes medication users as opposed to non‐users (pinteraction = 0.017, 0.033 and 0.014, respectively).ConclusionThe joint risk factor control is associated with an inverse association of CKD risk in an accumulative manner among individuals with obesity. Achieving ideal control over risk factors may effectively counterbalance the excessive risk of CKD typically associated with obesity.
Financial inclusion and financial crisis: Arguments, stylized facts and evidence
Authors: Ozili Peterson K. 
Published: August 2024

AbstractThe literature has examined the relationship between financial inclusion and financial stability, but no studies have examined the relationship between financial inclusion and financial crisis. This study examines the effect of financial inclusion on financial crisis using data from 28 countries from 2006 to 2017. Three stylised facts were established based on real‐world observation. One, the level of financial inclusion, in terms of number of bank depositors, decreases during domestic financial crisis. Two, the level of financial inclusion, in terms of ATM penetration, does not decrease during global and domestic financial crises. Three, the level of financial inclusion, in terms of number of bank branch, decreases during global and domestic financial crises and the contraction is stronger during a domestic financial crisis. Using the panel regression, logit and probit regression estimation methods, the empirical results show that low levels of financial inclusion, measured by fewer bank depositors and fewer bank branches, increase the likelihood that a financial crisis will occur. Low levels of financial inclusion, measured by fewer bank depositors, increase the likelihood that a financial crisis will occur in low financial‐inclusion countries. In contrast, greater ATM penetration increases the likelihood that a financial crisis will occur in low financial‐inclusion countries. The interaction analyses show that all indices of financial inclusion have a joint positive impact on financial crisis, implying that high levels of financial inclusion increases the likelihood that a financial crisis will occur.