bluuebunny/crossref_metadata_2025_split huggingface dataset:
Checklist clerking document improves health promotion among medical admissions
Authors: Cousins Sarah 
Published: October 2013

AbstractHealth promotion, as defined by the Ottawa Charter (1) is the 'process of enabling people to increase control over, and to improve their health'. Four life style risk factors: smoking, alcohol, obesity and physical activity are recognised as leading to health inequalities. These factors have implications at an individual, community and national level and the burden of disease has large cost implications for the NHS and country as a whole. Therefore the assessment of these risk factors, and the provision of good health promotion assist to address these problems. A pilot and initial audit demonstrated that assessment for the key life style risk factors and the provision of health promotion was poor. This quality improvement audit developed an easy to use, checklist based, amended clerking document. This improved both assessment and provision of health promotion and will be carried forward to influence the designing of a new clerking document.
Introduction of 'Anaphylaxis Packs' to improve patient safety in a hospital setting
Authors: Meade Susanna , Douglas Jenny 
Published: October 2013

AbstractThree reported episodes of anaphylaxis, where 1:1000 adrenaline was not immediately obtainable, triggered us to assess its availability on all adult wards. We found adrenaline was unavailable on 50% of audited wards. A questionnaire for doctors and nurses revealed lack of knowledge on both the management of anaphylaxis and location of emergency drugs. Given that anaphylaxis is a treatable but potentially fatal condition, we held a meeting to discuss the situation with senior pharmacists, resuscitation managers, and senior doctors. Our intervention was to advise the production of 'anaphylaxis packs' as part of the crash trolley kit. This was to be added to the laminated crash trolley check list and to include adrenaline, chlorphenamine, hydrocortisone, and the anaphylaxis algorithm. The aim was to improve ward stock, staff knowledge, and create a consistent location for emergency drugs, so minimising human error, and patient harm. With a PDSA approach we trialled the intervention on four pilot wards. The packs have now been dispersed trust-wide. Re-audit at four months showed 100% ward stock of anaphylaxis packs, more consistent drug location and improved staff knowledge. There were 17 coded incidents of anaphylaxis at this hospital in 2011, the actual figure likely being higher. We feel our project has greatly improved patient safety in this area.
Tectonometamorphic evolution of the Åreskutan Nappe – Caledonian history revealed by SIMS U–Pb zircon geochronology
Authors: Ladenberger Anna , Be'eri-Shlevin Yaron , Claesson Stefan , Gee David G. , Majka Jarosław , Romanova Irina V. 
Published: October 2013

Abstract

Secondary ionization mass spectrometry (SIMS) U–Pb dating of zircons from the Åreskutan Nappe in the central part of the Seve Nappe Complex of western central Jämtland provides new constraints on the timing of granulite–amphibolite-facies metamorphism and tectonic stacking of the nappe during the Caledonian orogeny. Peak-temperature metamorphism in garnet migmatites is constrained to
c.
442±4 Ma, very similar to the ages of leucogranites at 442±3 and 441±4 Ma. Within a migmatitic amphibolite, felsic segregations crystallized at 436±2 Ma. Pegmatites, cross-cutting the dominant Caledonian foliation in the Nappe, yield 428±4 and 430±3 Ma ages. The detrital zircon cores in the migmatites and leucogranites provide evidence of Late Palaeoproterozoic, Mesoproterozoic to Early Neoproterozoic source terranes for the metasedimentary rocks. The formation of the ductile and hot Seve migmatites, with their inverted metamorphism and thinning towards the hinterland, can be explained by an extrusion model in which the allochthon stayed ductile for a period of at least 10 million years during cooling from peak-temperature metamorphism early in the Silurian.

In our model, Baltica–Laurentia collision occurred in the Late Ordovician–earliest Silurian, with emplacement of the nappes far on to the Baltoscandian platform during the Silurian and early Devonian, Scandian Orogeny lasting until
c.
390 Ma.
Temporal Variation in Stable Isotopic Composition of Rainfall and Groundwater in a Tropical Dry Forest in the Northeastern Caribbean
Authors: Govender Y. , Cuevas E. , Sternberg L. D. S. , Jury M. R. 
Published: October 2013

AbstractKarst topography links rainfall to groundwater recharge; therefore, possible changes in the hydrology can play an important role in ecosystem function especially in tropical dry forests where water is the most limiting resource. This study investigates the temporal variation in isotopic composition (δ18O and δD values) of rainwater and groundwater in the Guánica Dry Forest of southwestern Puerto Rico. The study not only establishes a dataset of oxygen and hydrogen isotopic composition of rainwater to assist in local ecohydrological studies but also establishes the origin of rainfall in the semiarid region of the island. The geographical position of Puerto Rico in the northeastern Caribbean causes the study site to receive marine air masses from the North Atlantic Ocean and Caribbean Sea. This research documents the monthly to annual variability in stable isotopic composition of rainwater and estimates the source of groundwater recharge in the Guánica Dry Forest.To calculate the local meteoric water line (LMWL), the authors analyzed the isotopic signatures of rainwater, collected at near-monthly intervals from January 2008 to December 2011. The LMWL (δD = 7.79δ18O + 10.85) is close to the global meteoric water line (δD = 8.17δ18O + 11.27). Isotopic signatures of rainwater for the Guánica Dry Forest are consistent with southeastern Caribbean, where rainfall is of marine origin with an annual cycle contributed by sea surface temperature (SST) and significant intermonthly fluctuations due to rainfall and winds during tropical weather events. The d-excess values in the period of data collection (2008–11) respond to the rainfall–evaporation balance, with little seasonal cycle and strong pulsing events. Comparison of rain and groundwater isotopic compositions in the United Nations Educational, Scientific and Cultural Organization (UNESCO) Man and the Biosphere Programme (MAB) Guánica Dry Forest indicates that groundwater recharge is confined to rainfall events of more than 90 mm. Imbalances between rainfall and drought place cumulative stresses on ecosystems where plants and animals synchronize their growth phenology and reproduction to climatic patterns, especially in areas with variable annual cycles. Therefore, it is useful in ecohydrological studies to determine the origins and temporal dynamics of rainfall and groundwater recharge in the Caribbean, where predictions of climate models indicate drying trends.
Introducing Hysteresis in Snow Depletion Curves to Improve the Water Budget of a Land Surface Model in an Alpine Catchment
Authors: Magand Claire , Ducharne Agnès , Le Moine Nicolas , Gascoin Simon 
Published: October 2013

Abstract
The Durance watershed (14 000 km2), located in the French Alps, generates 10% of French hydropower and provides drinking water to 3 million people. The Catchment land surface model (CLSM), a distributed land surface model (LSM) with a multilayer, physically based snow model, has been applied in the upstream part of this watershed, where snowfall accounts for 50% of the precipitation. The CLSM subdivides the upper Durance watershed, where elevations range from 800 to 4000 m within 3580 km2, into elementary catchments with an average area of 500 km2. The authors first show the difference between the dynamics of the accumulation and ablation of the snow cover using Moderate Resolution Imaging Spectroradiometer (MODIS) images and snow-depth measurements. The extent of snow cover increases faster during accumulation than during ablation because melting occurs at preferential locations. This difference corresponds to the presence of a hysteresis in the snow-cover depletion curve of these catchments, and the CLSM was adapted by implementing such a hysteresis in the snow-cover depletion curve of the model. Different simulations were performed to assess the influence of the parameterizations on the water budget and the evolution of the extent of the snow cover. Using six gauging stations, the authors demonstrate that introducing a hysteresis in the snow-cover depletion curve improves melting dynamics. They conclude that their adaptation of the CLSM contributes to a better representation of snowpack dynamics in an LSM that enables mountainous catchments to be modeled for impact studies such as those of climate change.
Collaborative action around implementation in Collaborations for Leadership in Applied Health Research and Care: towards a programme theory
Authors: Rycroft-Malone Jo , Wilkinson Joyce , Burton Christopher R , Harvey Gill , McCormack Brendan , Graham Ian , Staniszewska Sophie 
Published: October 2013

Objectives In theory, greater interaction between researchers and practitioners should result in increased potential for implementation. However, we know little about whether this is the case, or what mechanisms might operate to make it happen. This paper reports findings from a study that is identifying and tracking implementation mechanisms, processes, influences and impacts in real time, over time in the Collaborations for Leadership in Applied Health Research and Care (CLAHRCs). Methods This is a longitudinal, realist evaluation case study. The development of the conceptual framework and initial hypotheses involved literature reviewing and stakeholder consultation. Primary data were collected through interviews, observations and documents within three CLAHRCs, and analysed thematically against the framework and hypotheses. Results The first round of data collection shows that the mechanisms of collaborative action, relationship building, engagement, motivation, knowledge exchange and learning are important to the processes and outcomes of CLAHRCs' activity, including their capacity for implementation. These mechanisms operated in different contexts such as competing agendas, availability of resources and the CLAHRCs’ brand. Contexts and mechanisms result in different impact, including the CLAHRCs’ approach to implementation, quality of collaboration, commitment and ownership, and degree of sharing and managing knowledge. Conclusion Emerging features of a middle range theory of implementation within collaboration include alignment in organizational structures and cognitive processes, history of partnerships, responsiveness and resilience in rapidly changing contexts. CLARHCs’ potential to mobilize knowledge may be further realized by how they develop insights into their function as collaborative entities.
Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae
Authors: Liddell Lauren C , Manthey Glenn M , Owens Shannon N , Fu Becky XH , Bailis Adam M 
Published: October 2013

Abstract

Background
In Saccharomyces cerevisiae, Rad59 is required for multiple homologous recombination mechanisms and viability in DNA replication-defective rad27 mutant cells. Recently, four rad59 missense alleles were found to have distinct effects on homologous recombination that are consistent with separation-of-function mutations. The rad59-K166A allele alters an amino acid in a conserved α-helical domain, and, like the rad59 null allele diminishes association of Rad52 with double-strand breaks. The rad59-K174A and rad59-F180A alleles alter amino acids in the same domain and have genetically similar effects on homologous recombination. The rad59-Y92A allele alters a conserved amino acid in a separate domain, has genetically distinct effects on homologous recombination, and does not diminish association of Rad52 with double-strand breaks.

Results
In this study, rad59 mutant strains were crossed with a rad27 null mutant to examine the effects of the rad59 alleles on the link between viability, growth and the stimulation of homologous recombination in replication-defective cells. Like the rad59 null allele, rad59-K166A was synthetically lethal in combination with rad27. The rad59-K174A and rad59-F180A alleles were not synthetically lethal in combination with rad27, had effects on growth that coincided with decreased ectopic gene conversion, but did not affect mutation, unequal sister-chromatid recombination, or loss of heterozygosity. The rad59-Y92A allele was not synthetically lethal when combined with rad27, stimulated ectopic gene conversion and heteroallelic recombination independently from rad27, and was mutually epistatic with srs2. Unlike rad27, the stimulatory effect of rad59-Y92A on homologous recombination was not accompanied by effects on growth rate, cell cycle distribution, mutation, unequal sister-chromatid recombination, or loss of heterozygosity.

Conclusions
The synthetic lethality conferred by rad59 null and rad59-K166A alleles correlates with their inhibitory effect on association of Rad52 with double-strand breaks, suggesting that this may be essential for rescuing replication lesions in rad27 mutant cells. The rad59-K174A and rad59-F180A alleles may fractionally reduce this same function, which proportionally reduced repair of replication lesions by homologous recombination and growth rate. In contrast, rad59-Y92A stimulates homologous recombination, perhaps by affecting association of replication lesions with the Rad51 recombinase. This suggests that Rad59 influences the rescue of replication lesions by multiple recombination factors.
Decreased miR-106a inhibits glioma cell glucose uptake and proliferation by targeting SLC2A3 in GBM
Authors: Dai Dong-Wei , Lu Qiong , Wang Lai-Xing , Zhao Wen-Yuan , Cao Yi-Qun , Li Ya-Nan , Han Guo-Sheng , Liu Jian-Min , Yue Zhi-Jian 
Published: October 2013

Abstract

Background
MiR-106a is frequently down-regulated in various types of human cancer. However the underlying mechanism of miR-106a involved in glioma remains elusive.

Methods
The association of miR-106a with glioma grade and patient survival was analyzed. The biological function and target of miR-106a were determined by bioinformatic analysis and cell experiments (Western blot, luciferase reporter, cell cycle, ntracellular ATP production and glucose uptake assay). Finally, rescue expression of its target SLC2A3 was used to test the role of SLC2A3 in miR-106a-mediated cell glycolysis and proliferation.

Results
Here we showed that miR-106a was a tumor suppressor miRNA was involved in GBM cell glucose uptake and proliferation. Decreased miR-106a in GBM tissues and conferred a poor survival of GBM patients. SLC2A3 was identified as a core target of miR-106a in GBM cells. Inhibition of SLC2A3 by miR-106a attenuated cell proliferation and inhibited glucose uptake. In addition, for each biological process we identified ontology-associated transcripts that significantly correlated with SLC2A3 expression. Finally, the expression of SLC2A3 largely abrogated miR-106a-mediated cell proliferation and glucose uptake in GBM cells.

Conclusions
Taken together, miR-106a and SLC2A3 could be potential therapeutic approaches for GBM.
Implementing disability evaluation and welfare services based on the framework of the international classification of functioning, disability and health: experiences in Taiwan
Authors: Chiu Wen-Ta , Yen Chia-Feng , Teng Sue-Wen , Liao Hua-Fang , Chang Kwang-Hwa , Chi Wen-Chou , Wang Yen-Ho , Liou Tsan-Hon 
Published: October 2013

Abstract

Background
Before 2007, the disability evaluation was based on the medical model in Taiwan. According to the People with Disabilities Rights Protection Act, from 2012 the assessment of a person’s eligibility for disability benefits has to be determined based on the International Classification of Functioning, Disability, and Health (ICF) framework nationwide. The purposes of this study were to: 1) design the evaluation tools for disability eligibility system based on the ICF/ICF-Children and Youth; 2) compare the differences of grades of disability between the old and new evaluation systems; 3) analyse the outcome of the new disability evaluation system.

Methods
To develop evaluation tools and procedure for disability determination, we formed an implementation taskforce, including 199 professional experts, and conducted a small-scale field trial to examine the feasibility of evaluation tools in Phase I. To refine the evaluation tools and process and to compare the difference of the grades of disability between new and old systems, 7,329 persons with disabilities were randomly recruited in a national population-based study in Phase II. To implement the new system smoothly and understand the impact of the new system, the collaboration mechanism was established and data of 168,052 persons who applied for the disability benefits was extracted from the information system and analysed in Phase III.

Results
The measures of the 43 categories for body function/structure components, the Functioning Scale of Disability Evaluation System for activities/participation components, and the needs assessment have been developed and used in the field after several revisions. In Phase II, there was 49.7% agreement of disability grades between the old and new systems. In Phase III, 110,667 persons with a disability received their welfare services through the new system. Among them, 77% received basic social welfare support, 89% financial support, 24% allowance for assistive technology, 7% caregiver support, 8% nursing care and rehabilitation services at home, and 47% were issued parking permits for persons with disability.

Conclusion
This study demonstrated that disability evaluation system based on the ICF could provide a common language between disability assessment, needs assessment and welfare services. However, the proposed assessment protocol and tools require additional testing and validation.
The Aeromonas salmonicida subsp. salmonicida exoproteome: global analysis, moonlighting proteins and putative antigens for vaccination against furunculosis
Authors: Vanden Bergh Philippe , Heller Manfred , Braga-Lagache Sophie , Frey Joachim 
Published: October 2013

Abstract

Background

Aeromonas salmonicida subsp. salmonicida, the etiologic agent of furunculosis, is a major pathogen of fisheries worldwide. Despite the identification of several virulence factors the pathogenesis is still poorly understood. We have used high-throughput proteomics to display the differences between in vitro secretome of A. salmonicida wild-type (wt, hypervirulent, JF5054) and T3SS-deficient (isogenic ΔascV, extremely low-virulent, JF2747) strains in exponential (GP) and stationary (SP) phases of growth.

Results
Among the different experimental conditions we obtained semi-quantitative values for a total of 2136 A. salmonicida proteins. Proteins of specific A. salmonicida species were proportionally less detected than proteins common to the Aeromonas genus or those shared with other Aeromonas species, suggesting that in vitro growth did not induce the expression of these genes. Four detected proteins which are unidentified in the genome of reference strains of A. salmonicida were homologous to components of the conjugative T4SS of A. hydrophila pRA1 plasmid. Polypeptides of three proteins which are specific to the 01-B526 strain were also discovered. In supernatants (SNs), the number of detected proteins was higher in SP (326 for wt vs 329 for mutant) than in GP (275 for wt vs 263 for mutant). In pellets, the number of identified proteins (a total of 1536) was approximately the same between GP and SP. Numerous highly conserved cytoplasmic proteins were present in A. salmonicida SNs (mainly EF-Tu, EF-G, EF-P, EF-Ts, TypA, AlaS, ribosomal proteins, HtpG, DnaK, peptidyl-prolyl cis-trans isomerases, GAPDH, Enolase, FbaA, TpiA, Pgk, TktA, AckA, AcnB, Mdh, AhpC, Tpx, SodB and PNPase), and several evidences support the theory that their extracellular localization was not the result of cell lysis. According to the Cluster of Orthologous Groups classification, 29% of excreted proteins in A. salmonicida SNs were currently poorly characterized.

Conclusions
In this part of our work we elucidated the whole in vitro exoproteome of hypervirulent A. salmonicida subsp. salmonicida and showed the secretion of several highly conserved cytoplasmic proteins with putative moonlighting functions and roles in virulence. All together, our results offer new information about the pathogenesis of furunculosis and point out potential candidates for vaccine development.
Proteomic profiling of serum samples from chikungunya-infected patients provides insights into host response
Authors: Puttamallesh Vinuth N , Sreenivasamurthy Sreelakshmi K , Singh Pradeep Kumar , Harsha H C , Ganjiwale Anjali , Broor Shobha , Pandey Akhilesh , Narayana Jayasuryan , Prasad T S Keshava 
Published: October 2013

Abstract

Background
Chikungunya is a highly debilitating febrile illness caused by Chikungunya virus, a single-stranded RNA virus, which is transmitted by Aedes aegypti or Aedes albopictus mosquito species. The pathogenesis and host responses in individuals infected with the chikungunya virus are not well understood at the molecular level. We carried out proteomic profiling of serum samples from chikungunya patients in order to identify molecules associated with the host response to infection by this virus.

Results
Proteomic profiling of serum obtained from the infected individuals resulted in identification of 569 proteins. Of these, 63 proteins were found to be differentially expressed (≥ 2-fold) in patient as compared to control sera. These differentially expressed proteins were involved in various processes such as lipid metabolism, immune response, transport, signal transduction and apoptosis.

Conclusions
This is the first report providing a global proteomic profile of serum samples from individuals infected with the chikungunya virus. Our data provide an insight into the proteins that are involved as host response factors during an infection. These proteins include clusterin, apolipoproteins and S100A family of proteins.