bluuebunny/crossref_metadata_2025_split huggingface dataset:
A retrospective, multicenter study evaluating the prognostic value of minor salivary gland histology in a large cohort of patients with primary Sjögren’s syndrome
Authors: Carubbi F , Alunno A , Cipriani P , Bartoloni E , Baldini C , Quartuccio L , Priori R , Valesini G , De Vita S , Bombardieri S , Gerli R , Giacomelli R 
Published: October 2014

Objective The objective of this report is to investigate the prognostic value of minor salivary glands (MSG) assessment, routinely performed with hematoxilin-eosin (H&E) staining, for the diagnosis of primary Sjögren’s syndrome (pSS). Methods We retrospectively evaluated clinical, serological and histological features of 794 pSS patients. H&E-stained sections were assessed using the Chisholm and Mason grading system and/or the focus score (FS). Results FS allowed the identification of a number of differences in the disease spectrum, and its prognostic role was further confirmed by quantifying the association between FS value and clinical/serological variables with binary logistic regression. Moreover, hypocomplementemia and FS resulted the only variables associated with lymphoma at univariate analysis, and FS appeared to be associated with lymphoma independently on complement fraction concentrations. Conversely, when patients were divided according to the Chisholm and Mason grading system, we failed to observe any significant difference between subgroups. Conclusion In addition to its diagnostic role, our data seem to support that the routine assessment of MSG-FS with H&E staining is useful to predict at the time of diagnosis the adverse outcomes, such as lymphoma and extraglandular manifestations, that complicate the pSS course. On this basis, it should be recommended that an MSG biopsy be performed even in those patients displaying clinical and serological criteria, allowing the diagnosis of pSS independent of histological status.
Diagnostic performance of CE-MRA in grading stenosis and therapy planning with TASC II classification
Authors: Wurz Clemens , Davari Amir , Ackermann Hanns , Vogl Thomas J 
Published: October 2014

Objectives To assess to what extent results of CE-MRA coincide with DSA in grading of stenosis and planning of therapy in patients with PAOD. Materials and methods Retrospectively, images of 71 PAOD-patients were studied by three observers. For evaluation, the lower limb was subdivided into 31 segments and categorized with TASCII-score. Results In grading stenosis, both modalities agreed in 93.26%. CE-MRA achieved sensitivity of 92.69% and specificity of 96.87% (κ = 0.88). The concordance of TASCII-classification was almost perfect. Conclusion CE-MRA is an excellent method for the evaluation of PAOD with some tendency to overrate the grade of stenosis.
Comparison of morphological and rheological conditions between conventional and eversion carotid endarterectomy using computational fluid dynamics – a pilot study
Authors: Demirel S , Chen D , Mei Y , Partovi S , von Tengg-Kobligk H , Dadrich M , Böckler D , Kauczor HU , Müller-Eschner M 
Published: October 2014

Purpose: To compare postoperative morphological and rheological conditions after eversion carotid endarterectomy versus conventional carotid endarterectomy using computational fluid dynamics. Basic methods: Hemodynamic metrics (velocity, wall shear stress, time-averaged wall shear stress and temporal gradient wall shear stress) in the carotid arteries were simulated in one patient after conventional carotid endarterectomy and one patient after eversion carotid endarterectomy by computational fluid dynamics analysis based on patient specific data. Principal findings: Systolic peak of the eversion carotid endarterectomy model showed a gradually decreased pressure along the stream path, the conventional carotid endarterectomy model revealed high pressure (about 180 Pa) at the carotid bulb. Regions of low wall shear stress in the conventional carotid endarterectomy model were much larger than that in the eversion carotid endarterectomy model and with lower time-averaged wall shear stress values (conventional carotid endarterectomy: 0.03–5.46 Pa vs. eversion carotid endarterectomy: 0.12–5.22 Pa). Conclusions: Computational fluid dynamics after conventional carotid endarterectomy and eversion carotid endarterectomy disclosed differences in hemodynamic patterns. Larger studies are necessary to assess whether these differences are consistent and might explain different rates of restenosis in both techniques.
Oral verapamil in paroxysmal supraventricular tachycardia recurrence control: a randomized clinical trial
Authors: Shaker Hossein , Jahanian Fatemeh , Fathi Marzieh , Zare MohammadAmin 
Published: October 2014

Background: Adenosine is the first-line medication in patients with paroxysmal supraventricular tachycardia. Because it is cleared so rapidly from the circulation, recurrence of paroxysmal supraventricular tachycardia after initial successful conversion may occur. Objective: This study was conducted to evaluate the role of oral verapamil administration to control early recurrences of paroxysmal supraventricular tachycardia after adenosine infusion. Methods: Patients with acute paroxysmal supraventricular tachycardia and no contraindications for adenosine or verapamil treatment were included in study. All patients received an adenosine protocol (6 mg rapid bolus intravenous injection followed by two repeated doses of 12 mg if necessary). Patients in the adenosine-only group did not received any other medications but patients in the adenosine/verapamil group received 40 mg verapamil orally immediately after converting the rhythm to sinus rhythm. All patients were followed up for 6 h in the acute care area of the emergency department under continuous cardiac monitoring. Results: A total of 113 patients were assessed for eligibility and 92 patients were randomized into two groups (adenosine only versus adenosine/verapamil). There was no statistically significant difference in paroxysmal supraventricular tachycardia recurrence rate between the two groups in the first 30 min after treatment. Recurrence rate was statistically significantly lower in the adenosine/verapamil group than in the adenosine-only group between 30 and 120 min after treatment and thereafter. Two patients in the adenosine-only group experienced flushing and one patient in the adenosine/verapamil group experienced decreased systolic blood pressure. Conclusion: Oral verapamil can decrease paroxysmal supraventricular tachycardia recurrence after successful control with intravenous adenosine.
Outpatient rigid cystoscopy: Is it acceptable to women?
Authors: Basak Sambita , Johnson Helen , Pradhan Ashish 
Published: October 2014

Objective: Flexible cystoscopy is commonly performed as an outpatient procedure whereas rigid cystoscopy is performed under general anaesthesia by urogynaecologists because of a perception that it is painful. The aim of this study is to assess women’s experience of outpatient cystoscopy (OC) with rigid cystoscope. Materials and methods: The first 56 women who attended our newly established OC service with rigid cystoscope were recruited prospectively for the study. They were requested to complete a questionnaire (with visual analogue scale) based on their expectations prior to the procedure. A post-procedure questionnaire was given to quantify their experience after the OC. Two patients were excluded from the study as they did not complete the questionnaire appropriately. Wilcoxon signed ranks test was used to compare the scores for comfort, pain and anxiety in the pre- and post-procedure questionnaires. Results: A total of 52/54 patients (96.3%) were fully satisfied at the end of the procedure as indicated in their visual analogue scale score of 10/10. Most patients found the procedure more comfortable and less painful than expected. There were no post-operative complications. Conclusion: OC with a rigid cystoscope is a well-tolerated, safe, cost-effective ambulatory service resulting in good patient experience.
Developing role models for health in the fire service: a pilot case study
Authors: Cheskin Lawrence J , Abel Michelle L , Bailey Maryanne M , Burnett Monica , Frutchey Robin , Goheer Attia , Ram Anita , Pollack Keshia M 
Published: October 2014

Summary Objectives The objectives of this case study were to develop role models for health in the fire service through knowledge and behaviour change, to improve the role models’ own health, and to facilitate behaviour change in other firefighters through their example. Design Volunteers interested in improving their own health and serving as role models to others in the fire service were identified at a statewide Maryland fire service leadership meeting. Setting Participants worked with the Johns Hopkins Weight Management Center to learn how to improve their own health and shared that knowledge with others in their fire departments. Participants Three Maryland fire service leaders were recruited at a leadership meeting with the goal of improving their own health and becoming role models. One participant dropped out shortly after beginning, while two male participants, aged 57 and 61, completed the study. Main outcome measures Quantitative measures were collected at baseline and 12 months, and included weight, blood pressure and fasting glucose and cholesterol. Semi-structured interviews were conducted approximately 14 months postintervention to determine the participants’ perceptions of their own health and impact on others in the fire service. Results Each participant had biweekly to monthly visits over a one-year period to learn the knowledge and skills that would assist him with improving dietary behaviours, increasing fitness and achieving a healthy weight. Case study participants experienced reductions in body weight (−13% and −11% of total body weight), glucose and blood pressure. Qualitative one-on-one interviews conducted postintervention with the participants revealed that they embraced their status as role models and felt their success inspired other firefighters. Their experiences suggest that role models can play an important role in helping firefighters increase self-efficacy, self-regulation and social support in the workplace environment. Conclusions These findings provide promising evidence for the use of role models to improve health, especially in the workplace.
Hospital-wide infection control practice and Meticillin-resistant Staphylococcus aureus (MRSA) in the intensive care unit (ICU): an observational study
Authors: Thompson David S , Workman Rella 
Published: October 2014

Summary Objectives To estimate trends in infection/colonisation with meticillin-resistant Staphylococcus aureus (MRSA) in an intensive care unit (ICU). Design Observational study of results of ICU admission and weekly screens for MRSA. Setting and Participants All ICU admissions in 2001–2012. Interventions ICU admissions were screened for MRSA throughout. In late 2006, screening was extended to the whole hospital and extra measures taken in ICU. Main outcome measures Prevalence of MRSA in ICU admissions and number acquiring MRSA therein. Results In all, 366 of 6565 admissions to ICU were MRSA positive, including 270 of 4466 coming from within the hospital in which prevalence increased with time prior to transfer to ICU. Prevalence in this group was 9.4% (8.2–10.6) in 2001–2006, decreasing to 3.4% (2.3–4.5) in 2007–2009 and 1.3% (0.6–2.0) in 2010–2012, p < 0.001, due to decreased prevalence in those spending >5 days on wards before ICU admission: 18.9% (15.6–22.2) in 2001–2006, 7.1% (4.0–10.2) in 2007–2009 and 1.6% (0.1–3.1) in 2010–2012, p < 0.001. In addition, 201 patients acquired MRSA within ICU, the relative risk being greater when known positives present: 4.34 (3.98–4.70), p < 0.001. Acquisition rate/1000 bed days decreased from 13.3 (11.2–15.4) in 2001–2006 to 3.6 (2.6–4.6) in 2007–2012, p < 0.0001. Of 41 ICU-acquired MRSA bacteraemias, 38 were in 2001–2006. The risk of bacteraemia in those acquiring MRSA decreased from 25% (18.1–31.9) in 2001–2006 to 6.1% (0–12.8) thereafter, p = 0.022. Conclusions Following better hospital-wide infection control, fewer MRSA-positive patients were admitted to ICU with a parallel decrease in acquisition therein. Better practice there reduced the risk of bacteraemia.
Retrospective observational study examining indications for hospitalisation among haemodialysis patients at one of the Ministry of Health Hospitals in Makkah, Saudi Arabia
Authors: Hassanien Amal A , Majeed Azeem , Watt Hilary 
Published: October 2014

Summary Objectives To examine the indications for hospitalisations among haemodialysis patients. Design A retrospective observational study. Setting Alnoor Kidney Centre in Al Noor Specialist Hospital, Makkah City, Saudi Arabia, which is a Ministry of Health hospital. Participants Participants were prevalent patients with end-stage renal disease on regular haemodialysis in 2011, who had received haemodialysis for more than three months. Each patient was followed up retrospectively, from the first date of initiating haemodialysis to the end of 2011. Main outcome measures (i) The primary reasons for hospital admissions and (ii) risk factors that increase the number of hospital admissions and which increase length of stay in hospital. Results The primary reasons for hospital admissions associated with increases in the length of stay in hospital were diseases of the circulatory system (which increased hospital bed days by 70%; 95% CI: 11–161%; p value = 0.01 compared to all other reasons). The risk factors that increased the number of hospital admissions per patient-year at risk were increasing age (incidence rate ratio [IRR] = 1.02 per 1 year of age; 95% CI: 1.01–1.03; p value = < 0.0001); receiving haemodialysis through a catheter compared to arteriovenous fistula (IRR = 2.55; 95% CI: 1.14–4.97; p value = 0.001) and diabetes as a cause of renal disease compared to hypertension (IRR = 1.84; 95% CI: 1.29–2.63; p value = 0.001). Conclusion Indications for hospitalisation and consequences of practices related to hospitalisation for haemodialysis patients should be studied in further research to provide a comprehensive evidence-based management policy for haemodialysis patients in Saudi Arabia.
Using stakeholder perspectives to develop an ePrescribing toolkit for NHS Hospitals: a questionnaire study
Authors: Lee Lisa , Cresswell Kathrin , Slee Ann , Slight Sarah P , Coleman Jamie , Sheikh Aziz 
Published: October 2014

Summary Objective To evaluate how an online toolkit may support ePrescribing deployments in National Health Service hospitals, by assessing the type of knowledge-based resources currently sought by key stakeholders. Design Questionnaire-based survey of attendees at a national ePrescribing symposium. Setting 2013 National ePrescribing Symposium in London, UK. Participants Eighty-four delegates were eligible for inclusion in the survey, of whom 70 completed and returned the questionnaire. Main outcome measures Estimate of the usefulness and type of content to be included in an ePrescribing toolkit. Results Interest in a toolkit designed to support the implementation and use of ePrescribing systems was high ( n = 64; 91.4%). As could be expected given the current dearth of such a resource, few respondents ( n = 2; 2.9%) had access or used an ePrescribing toolkit at the time of the survey. Anticipated users for the toolkit included implementation ( n = 62; 88.6%) and information technology ( n = 61; 87.1%) teams, pharmacists ( n = 61; 87.1%), doctors ( n = 58; 82.9%) and nurses ( n = 56; 80.0%). Summary guidance for every stage of the implementation ( n = 48; 68.6%), planning and monitoring tools ( n = 47; 67.1%) and case studies of hospitals’ experiences ( n = 45; 64.3%) were considered the most useful types of content. Conclusions There is a clear need for reliable and up-to-date knowledge to support ePrescribing system deployments and longer term use. The findings highlight how a toolkit may become a useful instrument for the management of knowledge in the field, not least by allowing the exchange of ideas and shared learning.
Risk for Revision After Anterior Cruciate Ligament Reconstruction Is Higher Among Adolescents
Authors: Faunø Peter , Rahr-Wagner Lene , Lind Martin 
Published: October 2014

Background: The number of children and adolescents with anterior cruciate ligament (ACL) reconstructions is increasing, and disturbing reports on high rerupture rates in this group have been noted. Purpose: To describe the outcome of ACL reconstruction in children and adolescents based on data from the Danish Knee Ligament Reconstruction Registry (DKRR). Study Design: Cohort study; Level of evidence, 3. Methods: Data were retrieved from the DKRR, a national population-based registry. The analysis was based on a population of 14,806 ACL-reconstructed patients. The outcome was evaluated using risk of ACL revision, subjective outcome score (Knee injury and Osteoarthritis Outcome Score [KOOS]), Tegner function score, and objective knee laxity. Three age groups were defined (A, <13 years; B, 13-15 years; and C, 15-20 years) and compared with D, patients ≥20 years (adults). There were 95 patients in group A, 327 in B, 2888 in C, and 11,496 in D. Results: There was a significantly increased risk of revision surgery in the age groups B (6.7%) and C (4.9%) compared with the adults in group D (2.0%). Objective knee laxity did not differ between the 4 groups. Groups A, B, and C had a higher score on the combined KOOS symptoms, pain, sport, and quality of life subscales (KOOS4; 79.6, 76.6, and 73.1, respectively) compared with the adults (69.7). Group B had higher KOOS quality of life (76.6) and sports (71.1) scores than did group C (73.1 and 66.4, respectively). The Tegner activity score did not differ between the 4 groups. No impact of the use of extracortical graft fixation was detected in the youngest age group. Conclusion: Study results indicated an increased risk of graft failure in patients between 13 and 20 years of age. This is in contrast to the better subjective and equal objective knee score found in the same age groups. Clinical Relevance: The new knowledge about the high revision rate among ACL-reconstructed teenagers is important for evidence-based preoperative information of ACL patients and their parents.
The formin DIAPH1 (mDia1) regulates megakaryocyte proplatelet formation by remodeling the actin and microtubule cytoskeletons
Authors: Pan Jiajia , Lordier Larissa , Meyran Deborah , Rameau Philippe , Lecluse Yann , Kitchen-Goosen Susan , Badirou Idinath , Mokrani Hayat , Narumiya Shuh , Alberts Arthur S. , Vainchenker William , Chang Yunhua 
Published: October 2014

Key PointsDIAPH1 (mDia1) is involved in both Rho-mediated actin polymerization and microtubule assembly and stability during proplatelet formation.
Interleukin 27 inhibits cytotoxic T-lymphocyte-mediated platelet destruction in primary immune thrombocytopenia
Authors: Zhou Hai , Qiu Ji-hua , Wang Tong , Yu Ying-yi , Liu Xue-na , Li Xin , Wang Ya-wen , Hou Yu , Li Li-zhen , Liu Xin-guang , Hou Ming , Peng Jun 
Published: October 2014

Key Points
IL-27 inhibits CTL cytotoxicity toward autologous platelets via decreasing granzyme B expression in ITP.
Neonatal bone marrow transplantation prevents bone pathology in a mouse model of mucopolysaccharidosis type I
Authors: Pievani Alice , Azario Isabella , Antolini Laura , Shimada Tsutomu , Patel Pravin , Remoli Cristina , Rambaldi Benedetta , Valsecchi Maria Grazia , Riminucci Mara , Biondi Andrea , Tomatsu Shunji , Serafini Marta 
Published: October 2014

Key Points
Allogeneic BMT into newborn MPS I mice allows high donor-derived hematopoietic engraftment and prevents bone deformities. Bones of transplanted MPS I mice show significant improvements at radiographic, microcomputed tomography, and histological analyses.
Nonlinearities distribution Laplace transform-homotopy perturbation method
Authors: Filobello-Nino Uriel , Vazquez-Leal Hector , Benhammouda Brahim , Hernandez-Martinez Luis , Hoyos-Reyes Claudio , Perez-Sesma Jose Antonio Agustin , Jimenez-Fernandez Victor Manuel , Pereyra-Diaz Domitilo , Marin-Hernandez Antonio , Diaz-Sanchez Alejandro , Huerta-Chua Jesus , Cervantes-Perez Juan 
Published: October 2014

Abstract
This article proposes non-linearities distribution Laplace transform-homotopy perturbation method (NDLT-HPM) to find approximate solutions for linear and nonlinear differential equations with finite boundary conditions. We will see that the method is particularly relevant in case of equations with nonhomogeneous non-polynomial terms. Comparing figures between approximate and exact solutions we show the effectiveness of the proposed method.
Alteration in basal and depolarization induced transcriptional network in iPSC derived neurons from Timothy syndrome
Authors: Tian Yuan , Voineagu Irina , Paşca Sergiu P , Won Hyejung , Chandran Vijayendran , Horvath Steve , Dolmetsch Ricardo E , Geschwind Daniel H 
Published: October 2014

Abstract
Background
Common genetic variation and rare mutations in genes encoding calcium channel subunits have pleiotropic effects on risk for multiple neuropsychiatric disorders, including autism spectrum disorder (ASD) and schizophrenia. To gain further mechanistic insights by extending previous gene expression data, we constructed co-expression networks in Timothy syndrome (TS), a monogenic condition with high penetrance for ASD, caused by mutations in the L-type calcium channel, Cav1.2.

Methods
To identify patient-specific alterations in transcriptome organization, we conducted a genome-wide weighted co-expression network analysis (WGCNA) on neural progenitors and neurons from multiple lines of induced pluripotent stem cells (iPSC) derived from normal and TS (G406R in CACNA1C) individuals. We employed transcription factor binding site enrichment analysis to assess whether TS associated co-expression changes reflect calcium-dependent co-regulation.

Results
We identified reproducible developmental and activity-dependent gene co-expression modules conserved in patient and control cell lines. By comparing cell lines from case and control subjects, we also identified co-expression modules reflecting distinct aspects of TS, including intellectual disability and ASD-related phenotypes. Moreover, by integrating co-expression with transcription factor binding analysis, we showed the TS-associated transcriptional changes were predicted to be co-regulated by calcium-dependent transcriptional regulators, including NFAT, MEF2, CREB, and FOXO, thus providing a mechanism by which altered Ca2+ signaling in TS patients leads to the observed molecular dysregulation.

Conclusions
We applied WGCNA to construct co-expression networks related to neural development and depolarization in iPSC-derived neural cells from TS and control individuals for the first time. These analyses illustrate how a systems biology approach based on gene networks can yield insights into the molecular mechanisms of neural development and function, and provide clues as to the functional impact of the downstream effects of Ca2+ signaling dysregulation on transcription.