bluuebunny/crossref_metadata_2025_split huggingface dataset:
An exploration of the solution of direct methanol fuel cell cost effectiveness
Authors: Wang Tengyi , Luo Zhiwei , Wang Changsheng , Li Yang , Chen Xi , Tang Yang , Wang Xinsheng , Zhou Zhiquan 
Published: August 2024

The work in this paper incorporated printed circuit board (PCB) technology into micro-direct methanol fuel cells (µDMFCs) and conjectured and verified the performance degradation factors of PCB current collectors in µDMFCs by testing different designed configuration µDMFCs. The experiment results showed that all kinds of PCB coating can benefit from the porous stainless-steel plates covering to a great extent. At the end of 48 h discharging, µDMFCs with porous stainless-steel plates between MEA and PCB coating achieved higher performance than those of the direct contacting series. It can be inferred from various types of experimental data that because of stainless-steel porous plate isolating, the impact of corrosion on the surface of the PCB electrode plate was reduced to a certain extent. The corrosion of the electrode plate was slowed down in the µDMFC discharging as a result of the passivation behavior on the iron surface and a decrease in corrosion current. Consequently, the attenuation of the PCB performance was delayed. The conclusion of this work explores a practical direction to enhance the cost-effectiveness of fuel cells, promoting the large-scale application of DMFCs in the future.
Smartwatch step counting: impact on daily step-count estimation accuracy
Authors: Düking Peter , Strahler Jana , Forster André , Wallmann-Sperlich Birgit , Sperlich Billy 
Published: August 2024

IntroductionThe effect of displayed step count in smartwatches on the accuracy of daily step-count estimation and the potential underlying psychological factors have not been revealed. The study aimed for the following: (i) To investigate whether the counting and reporting of daily steps by a smartwatch increases the daily step-count estimation accuracy and (ii) to elucidating underlying psychological factors.MethodsA total of 34 healthy men and women participants wore smartwatches for 4 weeks. In week 1 (baseline), 3 (follow-up 1), and 8 (follow-up 2), the number of smartwatch displayed steps was blinded for each participant. In week 2 (Intervention), the number of steps was not blinded. During baseline and follow-ups 1 and 2, the participants were instructed to estimate their number of steps four times per day. During the 4-week wash-out period between follow-ups 1 and 2, no feedback was provided. The Body Awareness Questionnaire and the Body Responsiveness Questionnaire (BRQ) were used to elucidate the psychological facets of the assumed estimation accuracy.ResultsThe mean absolute percentage error between the participants’ steps count estimations and measured steps counts were 29.49% (at baseline), 0.54% (intervention), 11.89% (follow-up 1), and 15.14% (follow-up 2), respectively. There was a significant effect between baseline and follow-up 1 [t (61.7) = 3.433, p &lt; 0.001] but not between follow-up 1 and follow-up 2 [t (60.3) = −0.288, p = 0.774]. Only the BRQ subscale “Suppression of Bodily Sensations” appeared to be significant at the Baseline (p = 0.012; Bonferroni adjusted p = 0.048) as a factor influencing step-count estimation accuracy.ConclusionThe counting and reporting of daily steps with a smartwatch allows improving the subjective estimation accuracy of daily step counts, with a stabilizing effect for at least 6 weeks. Especially individuals who tend to suppress their bodily sensations are less accurate in their daily step-count estimation before the intervention.
Byte-Sized Finance, Bushel-Sized Benefits: unraveling digital financial inclusion impact on China’s agricultural green development
Authors: Zhang Ludan , Qi Yanbin , Deng Xin 
Published: August 2024

IntroductionChina’s rural financial landscape has long been characterized by exclusion, leaving countless farmers without access to essential financial services. However, the advent of digital financial inclusion presents a promising solution, offering low-cost, high-penetration avenues to bolster agricultural sustainability. This paper unravels how “Byte-Sized Finance” can yield “Bushel-Sized Benefits” in China’s agricultural green development.MethodsMethodologically, we pioneer a novel system to gauge agricultural green development across 31 Chinese provinces spanning from 2013 to 2021. Empirically, employing fixed-effect, mediation effect, and spatial Durbin models.ResultsOur study reveals the intricate pathways through which digital financial inclusion influences agricultural green development. We find that it exerts its impact through the lenses of industrial structure and entrepreneurship, operating at both macro and micro levels. Furthermore, our analysis uncovers spatial spillover effects, shedding light on the differential roles played by these mechanisms across regions.DiscussionThis groundbreaking discovery underscores the transformative potential of leveraging digital financial inclusion to propel China toward agricultural green development. By shedding light on these crucial dynamics, our findings offer insights for policymakers, researchers, and practitioners striving to foster sustainability within China’s agricultural sector.
A systematic review on heavy metals contamination in Bangladeshi vegetables and their associated health risks
Authors: Nowar Abira , Islam Md. Hafizul , Islam Saiful , Jubayer Ahmed , Nayan Md. Moniruzzaman 
Published: August 2024

This systematic review aimed to identify the heavy metal contents of Bangladeshi vegetables and evaluate the subsequent health hazards of these heavy metals. Relevant studies were obtained from systematic searches of electronic databases. Average (mean) concentrations of Arsenic (AS), Lead (Pb), Cadmium (Cd), Chromium (Cr), Manganese (Mn), Nickel (Ni), Zinc (Zn), and Copper (Cu) in the vegetables were calculated on a fresh-weight basis. The health hazards associated with these metals’ intake were assessed using estimated daily intake (EDI) and carcinogenic and noncarcinogenic risks were measured as the target cancer risk (TR), target hazard quotient (THQ), and hazard index (HI). The results showed that the mean concentration of As, Pb, Cd, Cr, Mn, Ni, Zn, and Cu was 0.02–0.45, Not detectable (ND)-9.45, 0.00–0.51, 0.05–2.19, 0.28–43.43, Not detectable (ND)-5.80, 2.005–175.77, and 0.20–23.85 mg/kg of fresh vegetables respectively. The concentrations of As, Pb, and Cd were higher than the maximum allowable concentration (MAC) in the vegetables. However, all the metals were consumed daily in amounts below the maximum tolerable daily intake (MTDI) for all vegetables. THQ values for all metals except As and Pb were below the safety level (THQ &lt;1) in all the vegetables. Potential non-carcinogenic risk (THQ &gt;1) was posed by As and Pb in some vegetables like cabbage, string bean, bottle gourd, green papaya, sponge gourd, etc. The TR levels of As, Cd, Cr, and Ni were higher than the acceptable levels (10−4) for most vegetables, suggesting that long-term exposure to these toxic metals may raise the risk of developing various malignancies, including stomach and lung cancer. An integrated approach is required to address the contamination burden to preserve the health of the consumers.
Using preimplantation genetic testing for monogenic disease for preventing citrullinemia type 1 transmission
Authors: Wu Zubo , Liang Tao , Liu Yi , Ding Xiaofang , Shu Defeng 
Published: August 2024

AimThe aim of this study is to investigate if Preimplantation Genetic Testing (PGT) can effectively identify unreported variants according to American College of Medical Genetics and Genomics (ACMG)to prevent citrullinemia type 1 affection.DesignThis study involves a detailed case analysis of a family with history of citrullinemia type 1, focusing on the use of PGT for monogenic diseases (PGT-M). The genetic variants were identified using ACMG guidelines, and PGT was employed to prevent the inheritance of these variants. The study included haplotype analysis and Sanger sequencing to confirm the results.ResultsThe study identified previously unreported variations in the ASS1 gene causing citrullinemia type 1. PGT successfully prevented the transmission of these variants, resulting in the birth of a healthy fetus. However, challenges such as allele dropout (ADO) and gene recombination were encountered during haplotype analysis, which could potentially defeat the diagnosis. The study demonstrated that combining haplotype analysis with Sanger sequencing can enhance the accuracy of PGT.ConclusionPreimplantation Genetic Testing (PGT) targeting likely pathogenic and pathogenic variants in the ASS1 gene, as rated by ACMG, allows the birth of healthy infants free from citrullinemia type 1. Additionally, the establishment of single haplotypes and Sanger sequencing can reduce the misdiagnosis rate caused by allele dropout (ADO) and genetic recombination.
Integrative analysis reveals novel insights into juvenile idiopathic arthritis pathogenesis and shared molecular pathways with associated traits
Authors: Pudjihartono N. , Ho D. , O’Sullivan J. M. 
Published: August 2024

BackgroundJuvenile idiopathic arthritis (JIA) is an autoimmune joint disease that frequently co-occurs with other complex phenotypes, including cancers and other autoimmune diseases. Despite the identification of numerous risk variants through genome-wide association studies (GWAS), the affected genes, their connection to JIA pathogenesis, and their role in the development of associated traits remain unclear. This study aims to address these gaps by elucidating the gene-regulatory mechanisms underlying JIA pathogenesis and exploring its potential role in the emergence of associated traits.MethodsA two-sample Mendelian Randomization (MR) analysis was conducted to identify blood-expressed genes causally linked to JIA. A curated protein interaction network was subsequently used to identify sets of single-nucleotide polymorphisms (i.e., spatial eQTL SNPs) that regulate the expression of JIA causal genes and their protein interaction partners. These SNPs were cross-referenced against the GWAS catalog to identify statistically enriched traits associated with JIA.ResultsThe two-sample MR analysis identified 52 genes whose expression changes in the blood are putatively causal for JIA. These genes (e.g., HLA, LTA, LTB, IL6ST) participate in a range of immune-related pathways (e.g., antigen presentation, cytokine signalling) and demonstrate cell type-specific regulatory patterns across different immune cell types (e.g., PPP1R11 in CD4+ T cells). The spatial eQTLs that regulate JIA causal genes and their interaction partners were statistically enriched for GWAS SNPs linked with 95 other traits, including both known and novel JIA-associated traits. This integrative analysis identified genes whose dysregulation may explain the links between JIA and associated traits, such as autoimmune/inflammatory diseases (genes at 6p22.1 locus), Hodgkin lymphoma (genes at 6p21.3 [FKBPL, PBX2, AGER]), and chronic lymphocytic leukemia (BAK1).ConclusionOur approach provides a significant advance in understanding the genetic architecture of JIA and associated traits. The results suggest that the burden of associated traits may differ among JIA patients, influenced by their combined genetic risk across different clusters of traits. Future experimental validation of the identified connections could pave the way for refined patient stratification, the discovery of new biomarkers, and shared therapeutic targets.
Citrulline and ADI-PEG20 reduce inflammation in a juvenile porcine model of acute endotoxemia
Authors: Vonderohe Caitlin , Stoll Barbara , Didelija Inka , Nguyen Trung , Mohammad Mahmoud , Jones-Hall Yava , Cruz Miguel A. , Marini Juan , Burrin Douglas 
Published: August 2024

BackgroundArginine is a conditionally essential amino acid that is depleted in critically ill or surgical patients. In pediatric and adult patients, sepsis results in an arginine-deficient state, and the depletion of plasma arginine is associated with greater mortality. However, direct supplementation of arginine can result in the excessive production of nitric oxide (NO), which can contribute to the hypotension and macrovascular hypo-reactivity observed in septic shock. Pegylated arginine deiminase (ADI-PEG20, pegargiminase) reduces plasma arginine and generates citrulline that can be transported intracellularly to generate local arginine and NO, without resulting in hypotension, while maintaining microvascular patency. The objective of this study was to assess the efficacy of ADI-PEG20 with and without supplemental intravenous citrulline in mitigating hypovolemic shock, maintaining tissue levels of arginine, and reducing systemic inflammation in an endotoxemic pediatric pig model.MethodsTwenty 3-week-old crossbred piglets were implanted with jugular and carotid catheters as well as telemetry devices in the femoral artery to measure blood pressure, body temperature, heart rate, and respiration rate. The piglets were assigned to one of three treatments before undergoing a 5 h lipopolysaccharide (LPS) infusion protocol. Twenty-four hours before LPS infusion, control pigs (LPS; n=6) received saline, ADI-PEG20 pigs (n=7) received an injection of ADI-PEG20, and seven pigs (ADI-PEG20 + CIT pigs [n=7]) received ADI-PEG20 and 250 mg/kg citrulline intravenously. Pigs were monitored throughout LPS infusion and tissue was harvested at the end of the protocol.ResultsPlasma arginine levels decreased and remained low in ADI-PEG20 + CIT and ADI-PEG20 pigs compared with LPS pigs but tissue arginine levels in the liver and kidney were similar across all treatments. Mean arterial pressure in all groups decreased from 90 mmHg to 60 mmHg within 1 h of LPS infusion but there were no significant differences between treatment groups. ADI-PEG20 and ADI-PEG20 + CIT pigs had less CD45+ infiltrate in the liver and lung and lower levels of pro-inflammatory cytokines in the plasma.ConclusionADI-PEG20 and citrulline supplementation failed to ameliorate the hypotension associated with acute endotoxic sepsis in pigs but reduced systemic and local inflammation in the lung and liver.
Astrocyte-derived exosomal miR-378a-5p mitigates cerebral ischemic neuroinflammation by modulating NLRP3-mediated pyroptosis
Authors: Sun Ruiting , Liao Wenxin , Lang Ting , Qin Keyi , Jiao Keyan , Shao Le , Deng Changqing , She Yan 
Published: August 2024

ObjectiveThis study aimed to investigate the regulatory role of astrocyte-derived exosomes and their microRNAs (miRNAs) in modulating neuronal pyroptosis during cerebral ischemia.MethodsAstrocyte-derived exosomes were studied for treating cerebral ischemia in both in vitro and in vivo models. The effects of astrocyte-derived exosomes on neuroinflammation were investigated by analyzing exosome uptake, nerve damage, and pyroptosis protein expression. High throughput sequencing was used to identify astrocyte-derived exosomal miRNAs linked to pyroptosis, followed by validation via qRT‒PCR. The relationship between these miRNAs and NLRP3 was studied using a dual luciferase reporter assay. This study used miR-378a-5p overexpression and knockdown to manipulate OGD injury in nerve cells. The impact of astrocyte-derived exosomal miR-378a-5p on the regulation of cerebral ischemic neuroinflammation was assessed through analysis of nerve injury and pyroptosis protein expression.ResultsOur findings demonstrated that astrocyte-derived exosomes were internalized by neurons both in vitro and in vivo. Additionally, Astrocyte-derived exosomes displayed a neuroprotective effect against OGD-induced neuronal injury and brain injury in the ischemic cortical region of middle cerebral artery occlusion (MCAO) rats while also reducing pyroptosis. Further investigations revealed the involvement of astrocyte-derived exosomal miR-378a-5p in regulating pyroptosis by inhibiting NLRP3. The overexpression of miR-378a-5p mitigated neuronal damage, whereas the knockdown of miR-378a-5p increased NLRP3 expression and exacerbated pyroptosis, thus reversing this neuroprotective effect.ConclusionAstrocyte-derived exosomal miR-378a-5p has a neuroprotective effect on cerebral ischemia by suppressing neuroinflammation associated with NLRP3-mediated pyroptosis.Further research is required to comprehensively elucidate the signaling pathways by which astrocyte-derived exosomal miR-378a-5p modulates neuronal pyroptosis.
Prediction of adenomyosis according to revised definitions of morphological uterus sonographic assessment features
Authors: Yavuz Onur , Akdöner Asli , Özgozen Mehmet Eyüphan , Ertan Begüm , Kurt Sefa , Ulukuş Emine Cagnur , Güney Mehmet 
Published: August 2024

ObjectivesThis study aimed to predict the diagnosis of adenomyosis by revised definitions of morphological uterus sonographic assessment (MUSA) features in individuals who had hysterectomy.MethodsThis was retrospective cohort research conducted at a tertiary facility. Between January 2022 and January 2023, 196 individuals who had hysterectomy were analyzed in the research. The revised definitions of MUSA features of the adenomyosis approach were used to record the direct and indirect results of the sonography. The cases were classified as Group 1 (adenomyosis; n = 40, 20.4%) and Group 2 (control; n = 156, 79.6%) according to histopathology reports.ResultsHyperechogenic islands and echogenic subendometrial buds and lines were the most predictive direct features (p = 0.02). Globular uterus and irregular junctional zone were the most predictive indirect features (p = 0.04; p = 0.03, respectively). Among all indirect features, the globular uterus was the most predictive (p = 0.02). Total feature &gt;4 was determined as the significant cutoff value to predict adenomyosis (p &lt; 0.001).ConclusionThis study shows that combinations with a total number of features &gt;4 can be practically used in the evaluation of adenomyosis using the revised definitions of MUSA features.
Causality between various cytokines and asthma: a bidirectional two-sample Mendelian randomization analysis
Authors: Zheng Yansen , Chen Qi , Shi Xiaqing , Lei Lei , Wang Donglin 
Published: August 2024

BackgroundMany studies have shown that cytokines play an important role in the pathogenesis of asthma, but their biological effects on asthma remain unclear. The Mendelian randomization (MR) method was used to evaluate the causal relationship between various cytokines [such as interleukins (ILs), interferons (IFNs), tumor necrosis factors (TNFs), colony-stimulating factors (CSFs), transforming growth factor (TGF), etc.,] and asthma.MethodsIn this study, inverse variance weighting was used to evaluate the causal relationship between asthma and cytokines. In addition, the reliability of the results is ensured by multiple methods such as MR-Egger, weighted median, MR-Raps, MR-Presso, and RadialMR, as well as sensitivity analysis.ResultsThe results showed that none of the 11 cytokines was associated with the risk of asthma. In contrast, asthma can increase levels of IL-5 [odds ratio (OR) = 1.112, 95% confidence interval (CI): 1.009–1.224, P = 0.032] and IL-9 (OR = 1.111, 95% CI: 1.013–1.219, P = 0.025).ConclusionGenetically predicted asthma was positively associated with elevated levels of IL-5 and IL-9, indicating the downstream effects of IL-5 and IL-9 on asthma. Medical treatments can thus be designed to target IL-5 and IL-9 to prevent asthma exacerbations.
Longitudinal evaluation of manufacturer-specific differences for high-sensitive CRP EQA results
Authors: Weiss Nathalie , Vierbaum Laura , Kremser Marcel , Kaufmann-Stoeck Anne , Kappler Silke , Ballert Silvia , Kabrodt Kathrin , Hunfeld Klaus-Peter , Schellenberg Ingo 
Published: August 2024

BackgroundC-reactive protein (CRP) is an established serum biomarker for different pathologies such as tissue injury and inflammatory events. One rising area of interest is the incorporation of low concentrations of CRP, so called high-sensitive (hs-) CRP, in the risk assessment and treatment monitoring of cardiovascular diseases (CVDs). Many research projects and the resulting meta-analyses have reported controversial results for the use of hs-CRP, especially in the risk assessment of CVDs. However, since these analyses used different assays to detect hs-CRP, it is important to assess the current level of assay harmonization.MethodsThis paper analyzes data from 17 external quality assessment (EQA) surveys for hs-CRP conducted worldwide between 2018 and 2023. Each EQA survey consisted of two blinded samples. In 2020 the sample material changed from pooled serum to single-donor samples. The aim was to assess the current status of assay harmonization by a manufacturer-based approach, taking into consideration the clinical decision limits for hs-CRP risk-stratification of CVDs as well as the scatter of results.ResultsOur analyses show that harmonization has increased in recent years from median differences of up to 50% to below 20%, with one exception that showed an increasing bias throughout the observed period. After changing sample materials from pools to single-donor samples, the coefficient of variation decreased to below 10% with one exception. Nevertheless, even these differences in the clinical setting could lead to disparate classification of patients depending on the assay used.ConclusionWhile there was a positive trend towards harmonization, meta-analysis of different risk-score publications should stratify their analysis by assay to account for the manufacturer-specific differences observed in this paper. Furthermore, assays are currently traceable to different international standard preparations, which might have a negative impact on future harmonization.
From inner to dyadic connection: the role of mindfulness in mother–infant interaction during the first year of life
Authors: Passaquindici Ilenia , Pastore Massimiliano , Nardozza Odette , Lionetti Francesca , D’Urso Giulio , Palumbo Riccardo , Fasolo Mirco , Spinelli Maria 
Published: August 2024

IntroductionMother–infant attunement is fundamental to supporting infant socio-emotional development. Based on the assumption that we connect better with others if we are aware of and connected with our own experience, mindfulness could affect the maternal ability to attune to the infant. However, little is known about this topic in the first year of life. Study 1 aimed to investigate the role of maternal dispositional mindfulness and mindful parenting in mother–infant physiological and behavioral attunement at 3 months of age. Study 2 aimed to explore the effect of a mindfulness-based intervention not specific to parenting experience on mother–infant behavioral and physiological attunement and on maternal wellbeing at 9 months of age.MethodsIn Study 1, mother–infant (n = 67) behavioral and physiological attunement (i.e., co-regulation and RSA) were collected simultaneously each 20 s during face-to-face interaction. Mothers completed questionnaires about their dispositional mindfulness and mindful parenting. In Study 2, mother–infant dyads were randomly divided into a control (n = 20) and an intervention group (n = 29). The intervention group attended a 5-week mindfulness-based intervention. At T1 and T2, the same procedure described in Study 1 was applied and mothers reported about their wellbeing.ResultsResults showed that maternal mindfulness was associated with high physiological and behavioral attunement at 3 months and with more positive maternal behaviors and less stress at 9 months. Analysis evidenced a slight improvement in the intervention group in maternal dispositional mindfulness and a reduction in parenting stress at T2.DiscussionFindings from both studies suggested that maternal mindfulness could represent a protective factor that could support mothers in fostering better dyadic interactions with their infants. The implementation of mindfulness-based interventions for mothers could have preventive and clinical implications.