bluuebunny/crossref_metadata_2025_split huggingface dataset:
Issues in the design of tissue-engineered collagen constructs and some approaches to their solution: A review
Authors: Farion I. A. , Burdukovskii V. F. 
Published: July 2024

This review article analyzes modern literature sources on the design of bioinks and tissue-engineered constructs on the basis of soluble forms of collagen, including gelatin. The choice of soluble forms of collagen as a biopolymer basis for bioinks and this type of constructs is determined by their unique biocompatibility, bioresorbability, as well as the presence of adhesive sites (motifs) for binding cells with their subsequent proliferation and organ or tissue maturation. However, the poor mechanical properties of products derived from soluble collagens, rapid biodegradation, tendency to lose the solubility of highly viscous solutions when stored or with pH increase limit their application in tissue engineering. The use of more stable low-viscosity collagen solutions does not enable the creation of dimensionally stable tissue-engineered constructs. It is shown that the introduction of various water-soluble biocompatible polymeric additives into hydrogels on the basis of soluble collagens allows the above-mentioned problems to be solved, as well as providing a means to customize the required characteristics of bioinks and tissue-engineered constructs. The additives that improve their characteristics include biopolymers: silk sericin and fibroin, as well as alginates and fibrinogen, which can form cross-links in the presence of Ca2+. This type of crosslinking is shown to further improve the performance of these constructs. All of these biopolymers are commercially available. The article comparatively analyzes approaches to stabilizing the shape, improving the mechanical properties, and adjusting the bioresorption time of 3D printed tissue-engineered constructs during organ or tissue maturation.
Prethermalization in coupled one-dimensional quantum gases
Authors: Łebek Maciej , Panfil Miłosz , Konik Robert M. 
Published: July 2024

We consider the problem of the development of steady states in one-dimensional Bose gas tubes that are weakly coupled to one another through a density-density interaction. We analyze this development through a Boltzmann collision integral approach. We argue that when the leading order of the collision integral, where single particle-hole excitations are created in individual gases, is dominant, the state of the gas evolves first to a non-thermal fixed point, i.e. a prethermalization plateau. This order is dominant when a pair of tubes are inequivalent with, say, different temperatures or different effective interaction parameters, \gammaγ. When both tubes are in the strongly interacting regime we additionally characterize this non-thermal prethermalization plateau by constructing the quasi-conserved quantities that control the existence of this plateau as well as the associated generalized Gibbs ensemble.
A new look at the distributive incidence of Chile’s means-tested water subsidy scheme
Authors: Errázuriz Clemente , Gómez-Lobo Andrés 
Published: July 2024

ABSTRACT

Chile employs subsidies to reduce the bill paid by lower-income households for piped water and sanitation. Previous research has concluded that this system has a lower Gini coefficient (0.29) than expected, meaning it is not appropriately targeted to low-income households. This paper takes a new approach to assessing the subsidy system, taking advantage of Chile’s 2016 adoption of a new means-test instrument in the welfare system. We refine the analysis by (1) discarding data arising from rural unconnected households, on the theory that they cannot feasibly be connected to piped water systems and thus cannot use the subsidy; (2) recognizing Chile’s use of an equivalence scale to adjust household incomes for household size and relative disadvantage, and (3) adjusting the analysis to reflect the different tariff levels across the country. With these adjustments, the Gini coefficient of benefits increases to 0.47, meaning that the Chilean subsidy program is in fact meeting its goal of targeting assistance to those who need it. In other words, previous research has underestimated the targeting progressivity of the Chilean subsidy scheme. We also use the Shapley value to apportion the improvement from 2015 to 2022 among the various changes introduced in the analysis.
Effect of Ozone Therapy on Diabetes-related Foot Ulcer Outcomes: A Systematic Review and Meta-analysis
Authors: Izadi Morteza , Jafari-Oori Mehdi , Eftekhari Zohre , Jafari Nematollah Jonaidi , Maybodi Mohamad Kazem-Emami , Heydari Soleyman , Vahedian-Azimi Amir , Atkin Stephen L. , Jamialahmadi Tannaz , Sahebkar Amirhossein 
Published: July 2024

Purpose:
This study aimed to assess the effectiveness of ozone therapy in treating Diabetes-related
Foot Ulcer (DFU) and its outcomes.

Methods:
A systematic search was conducted in PubMed/MEDLINE, Scopus, Web of Science, and ProQuest
databases for published studies evaluating the use of ozone as an adjunct treatment for DFU, from inception to
December 21, 2022. The primary outcome measure was the change in wound size after the intervention compared
to pretreatment. Secondary outcomes included time to complete ulcer healing, number of healed patients,
adverse events, amputation rates, and hospital length of stay. Quantitative data synthesis for the meta-analysis
was performed using a random-effects model and generic inverse variance method, while overall heterogeneity
analysis was conducted using a fixed-effects model. Interstudy heterogeneity was assessed using the I2 index
(>50%) and the Cochrane Q statistic test. Sensitivity analysis was performed using the leave-one-out method.

Results:
The meta-analysis included 11 studies The meta-analysis included 11 studies comprising 960 patients with DFU. The results demonstrated a
significant positive effect of ozone therapy on reducing foot ulcer size (Standardized Mean Difference (SMD):
-25.84, 95% CI: -51.65 to -0.04, p = 0.05), shortening mean healing time (SMD: -38.59, 95% CI: -51.81 to -25.37,
p < 0.001), decreasing hospital length of stay (SMD: -8.75, 95% CI: -14.81 to -2.69, p < 0.001), and reducing amputation
rates (Relative Risk (RR): 0.46, 95% CI: 0.30-0.71, p < 0.001), compared to standard treatment.

Conclusion:
This meta-analysis indicates that ozone therapy has additional benefits in expediting complete
DFU healing, reducing the amputation rates, and decreasing hospital length of stay, though its effects do not
differ from standard treatments for complete ulcer resolution. Further research is needed to address the heterogeneity
among studies and to better understand the potential beneficial effects of ozone therapy.

Registration Number::
CRD42023386828.
Effects of Sex Differences and Combined Use of Clozapine on Initial Dosage Optimization of Valproic Acid in Patients with Bipolar Disorder
Authors: Shen Wei , Hu Ke , Shi Hao-Zhe , Jiang Lei , Zhang Yi-Jia , He Su-Mei , Zhang Cun , Chen Xiao , Wang Dong-Dong 
Published: July 2024

Background:
Due to the narrow therapeutic window and large pharmacokinetic variation of valproic
acid (VPA), it is difficult to make an optimal dosage regimen. The present study aims to optimize the initial
dosage of VPA in patients with bipolar disorder.

Methods:
A total of 126 patients with bipolar disorder treated by VPA were included to construct the VPA
population pharmacokinetic model retrospectively. Sex differences and combined use of clozapine were found
to significantly affect VPA clearance in patients with bipolar disorder. The initial dosage of VPA was further
optimized in male patients without the combined use of clozapine, female patients without the combined use
of clozapine, male patients with the combined use of clozapine, and female patients with the combined use of
clozapine, respectively.

Results:
The CL/F and V/F of VPA in patients with bipolar disorder were 11.3 L/h and 36.4 L, respectively. It
was found that sex differences and combined use of clozapine significantly affected VPA clearance in patients
with bipolar disorder. At the same weight, the VPA clearance rates were 1.134, 1, 1.276884, and 1.126 in male
patients without the combined use of clozapine, female patients without the combined use of clozapine, male
patients with the combined use of clozapine, and female patients with the combined use of clozapine, respectively.
This study further optimized the initial dosage of VPA in male patients without the combined use of
clozapine, female patients without the combined use of clozapine, male patients with the combined use of
clozapine, and female patients with the combined use of clozapine, respectively.

Conclusion:
This study is the first to investigate the initial dosage optimization of VPA in patients with bipolar
disorder based on sex differences and the combined use of clozapine. Male patients had higher clearance,
and the recommended initial dose decreased with increasing weight, providing a reference for the precision
drug use of VPA in clinical patients with bipolar disorder.
Modified Microwave-assisted Solid-liquid Halex Reaction using Phosphonium Salt as Efficient and Robust Phase Transfer Catalyst
Authors: Mohite Dhanaji M. , Chavhan Pandurang M. , Basu Arghya 
Published: July 2024

:
A modified solid-liquid halex reaction was developed in the presence of a robust
phase transfer catalyst under microwave conditions. A fast, mild, and practical microwave-
assisted synthesis of 2,3-difluoro-5-chloropyridine 3 starting from 2,3,5-
trichlorpyridine 1 and spray-dried KF in polar aprotic solvent was developed. The addition
of Tetrakis (piperidino) phosphonium chloride as phase transfer catalyst A was studied under
microwave irritation (450W) and increased the yield and significantly reduced the reaction
time in contrast to the conventional heating procedure. The highest reaction rate was
observed at 5 wt% phase transfer phosphonium salt catalyst to 2,3,5-trichloropyridine 1.
Preparation and Evaluation of Nanoemulsion Formulation Containing Kojic Acid and Kojyl 3-aminopropylphosphonic Acid
Authors: Chang Nai-Fang , Wu Pey-Shiuan , Yang Hsiang-Ju , Zheng Ya-Min , Lin Chih-Chien 
Published: July 2024

Background:
The kojyl 3-aminopropylphosphonic acid (KAP) was synthesized by
kojic acid (KA) with a 3-aminopropylphosphonic acid. Which is more stable than KA and
showed better skin penetration and anti-pigmentation efficacy in melanocytes. However, up till
now, there have been no studies aimed at incorporating KAP into an emulsion system and evaluating
its effectiveness.

Objective:
We develop a novel skin-lightening agent using KAP as the active ingredient and a
low-cytotoxic nanoemulsion as the delivery system in this study.

Method:
The sorbitan monooleate and polysorbate surfactants with polyethylene glycol (PEG)
co-surfactant were used to generate a nanoemulsion system.

Result:
The transparency and particle size stability over various storage times indicate that the
formulated nanoemulsions are suitable for long-term storage. Besides, results demonstrate that
the anti-pigmentation function of KA and KAP-containing nanoemulsions (NE-KA and NEKAP)
evidently outperformed that of the non-packed KA and KAP group. Despite having the
lowest concentration among other treatments, NE-KAP was able to reduce melanin content to
approximately 80% of the blank.

Conclusion:
Our findings suggest that this newly developed nanoemulsion containing KAP
could potentially serve as a sustainable alternative to hydroquinone for treating dermal hyperpigmentation
disorders in future applications.
The Pattern of Anemia in Pediatric Solid Tumors Prior to and after Chemotherapy- A Retrospective Cohort Study
Authors: Aron Tomal Barman , Roy Sagor Kumar , Mao Shuting , Li Bai , Richard Seidu A. , Liu Yufeng 
Published: July 2024

Background:
Solid pediatric tumors refer to cancers that affect children and adoles-cents, and they present unique challenges due to their distinct biological characteristics and their vulnerability to young patients. This study aims to shed light on addressing anemia and the causes of anemia in patients with solid pediatric tumors.

Materials and Methods:
This retrospective cohort comprised 200 healthy children as controls and 235 patients with solid tumors. The study was conducted at first Affiliated Hospital of Zhengzhou University between January 2020 and June 2023. We evaluated different parameters of blood components in controls and patients with solid tumors such as medulloblastoma, neuroblastoma, rhabdomyosarcoma, germ cell tumors, hepatoblastoma and nephroblastoma before and patients with only these tumors 3 weeks after the first cycle of chemotherapy. Further, we evaluated the relationship between serum ferritin and the weight of patients and assessed the relationship between anemia and metastasis to the bone marrow in patients with neuroblastoma and hepatoblastoma.

Results:
We observed various combinations of derangements in blood parameters such as hemoglobin, red blood cells, mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, hematocrit, red cell distribution width, white blood cells, and plate-let in medulloblastoma, neuroblastoma, rhabdomyosarcoma, germ cell tumors, hepatoblastoma and nephroblastoma before and 3 weeks after first cycle of chemotherapy. We found a significant correlation between serum ferritin levels and weight in neuroblastoma patients who are ≤ 2 years (p = 0.022). Involvement of tumor cells in bone marrow correlates with decreased Hb level in both neuroblastoma (CI = 93.21-106.68, p = 0.001) and hepatoblastoma (CI = 113.36-121.00, p = 0.001).

Conclusion:
Anemia may manifest as an early symptom in neuroblastoma, hepatoblastoma, and nephroblastoma. Also, anemia may be worse in patients with neuroblastoma and hepatoblastoma after chemotherapy and might warrant anemia therapy.
Rings in “Lead-like Drugs”
Authors: Mendes Lampert Luca , Ruszczyk Machado Bruno , Rocha Joaquim Angelica , Fumagalli Fernando 
Published: July 2024

Background:
Lead-like drugs, which present molecular weight (MW) < 300 Da, occupy
an important space in the pharmaceutical area. Most of these small molecules have ring systems,
which are important for their physicochemical properties and biological activity. Previous studies
have evaluated ring systems in historic drugs or drug candidates in clinical trials.

Objective:
The purpose of this work was to analyze ring systems, focusing on this group of drugs
with MW < 300 Da, to obtain specific insights.

Methods:
The lead-like drugs (n = 219) were obtained from previous publications and the new FDA
drug approvals were obtained after that and analyzed using the DataWarrior software.

Results:
Most of the lead-like drugs (> 92%) present one or two rings, with the benzene ring and
heterocycle ring systems being predominant. Pyridine, imidazole, piperidine, 4,5-dihydro-1Himidazole,
and indole are the most frequent heterocycles in this set. The higher frequency of the 4,5-
dihydro-1H-imidazole ring in the lead-like drugs is worth noting, as it is not observed in other drugs.
The introduction of new rings has been similar in the lead-like drugs and the historic drugs, over the
years; an example would be the 1,2,4-thiadiazinane 1,1-dioxide, which is present in the antimicrobial
Taurolidine, a lead-like drug, and engages in the metabolic activation of the drug.

Conclusion:
In general, the ring systems in the lead-like drugs appear to follow similar patterns to
the historic drugs. Additionally, few new ring systems are being introduced, which suggests that this
is an emergent field to be explored in drug discovery.
Robust U-HPLC Method Development of Desonide and its Application to In Vitro Release Testing (IVRT) of Topical Cream Products
Authors: Jayadev Seelam , Yusuff Ismail 
Published: July 2024

Background:
The study focuses on establishing In Vitro Release Testing (IVRT) parameters
for Desonide cream, following the guidelines of the Topical Classification System (TCS),
to assess the bioequivalence between the Reference Listed Drug (RLD) and test.

Aim:
This study aimed to develop a reliable IVRT method using Franz diffusion cells. An environmentally
friendly U-HPLC method was created to analyze Desonide in the samples.

Objectives:
To evaluate the drug release in Desonide products in accordance with SUPAC guidance,
quantify the drug concentration using an analytical method, as per bioanalytical method validation
guidelines, and ensure that the results meet the acceptance criteria. Linearity was established from
0.50 μg/mL to 40 μg/mL with acceptable regression values. Precision was confirmed three times,
with an average % RSD of below 15% for 3 sets of 6QC level sample preparations. Stability tests
demonstrated Desonide stability in receptor fluid (LLOQ and ULOQ) for 72 hours at 2-8°C and 25°C.
Autosampler stability at LQC and HQC levels was proven at 25°C for 72 hours. Additionally, the
stock solution remained stable at both 25°C and 2-8°C for 72 hours.

Methods:
The study involved evaluating the dosing regimen, release medium, and membrane
while optimizing the U-HPLC method based on three variables including column temperature, mobile
phase composition, and flow rate. After experimentation, it was determined that Nylon membrane
and 0.9% NaCl: Methanol release media (70:30 v/v) with 1000 mg dose were used to maximize
the release profile of desonide.

Results:
The created explanatory strategy is precise, delicate, and exact for measuring Desonide,
with satisfactory Limits of Location LOD and Lower Limits of Measurement LLOQ measured at
0.15 and 0.50 ng /mL, respectively. The Regression coefficient r2 was identified to be 0.9996. The
degree of Desonide measurement lessening was considered palatable, basically since the recuperation
was underneath 30.00, additionally due to the favourable linear relationship watched within
the Desonide discharge rates amid the IVRT study.

Conclusion:
All three generic products analyzed were found to be equivalent to the RLD, meeting
for "sameness" outlined in the FDA's SUPAC-SS guidance. A novel U-HPLC method was developed
for Desonide, covering the range from 0.5 to 40 μg/ml, with intra and inter-day variability below
2% RSD. Additional characterizations were established, and the stability of Desonide was successfully
determined.
GenRepAI: Utilizing Artificial Intelligence to Identify Repeats in Genomic
Suffix Trees
Authors: Kaniwa Freeson 
Published: July 2024

Background:
The human genome is densely populated with repetitive DNA sequences
that play crucial roles in genomic functions and structures but are also implicated in over 40 human
diseases. The computational challenge of identifying and characterizing these repeats is significant
due to the complexity and size of the genome, which are overwhelming traditional algorithms.

Methods:
To address these challenges, we propose GenRepAI, a deep learning framework to navigate
and analyze genomic suffix trees. GenRepAI employs supervised machine learning classifiers
trained on labeled datasets of repeat annotations and unsupervised anomaly detection to identify
novel repeat sequences. The models are trained using convolutional neural networks (CNNs), long
short-term memory networks (LSTMs), and vision transformers to classify and annotate repeats
within the human genome.

Results:
GenRepAI is designed to comprehensively profile repeats that underlie various neurological
diseases, allowing researchers to identify pathogenic expansions. The framework will integrate
into existing genomic analysis pipelines, with the capability to screen patient genomes and highlight
potential causal variants for further validation.

Conclusion:
GenRepAI is set to become a foundational tool in genomics, leveraging artificial intelligence
to enhance the characterization of repetitive sequences. It promises significant advancements
in the molecular diagnosis of repeat expansion disorders and contributes to a deeper understanding
of genomic structure and function, with broad applications in personalized medicine.
Reliability and Factor Structure of the Well-Being and Respect for Human Rights Questionnaire in Measuring Caregivers' Perception
Authors: Atzeni Michela , Carta Mauro G. , Primavera Diego , Aviles Gonzales Cesar Ivan , Galletta Maura , Marchegiani Sonia , Carboni Giorgio , Sancassiani Federica , Nonnis Marcello , Urban Antonio , Cantone Elisa , Nardi Antonio E. , Preti Antonio , Atzeni Michela , Carta Mauro G. , Primavera Diego , Aviles Gonzales Cesar Ivan , Galletta Maura , Marchegiani Sonia , Carboni Giorgio , Sancassiani Federica , Nonnis Marcello , Urban Antonio , Cantone Elisa , Nardi Antonio E. , Preti Antonio 
Published: July 2024

BackgroundThis study has investigated perceptions of respect for users' rights among informal caregivers in mental healthcare settings, aligning with the guidelines outlined in the United Nations Convention on the Rights of Persons with Disabilities (CRPD) and the World Health Organization QualityRights initiative. The study has employed the questionnaire on Well-being at Work and Respect for Rights (WWRR) among informal caregivers and tested whether the questionnaire's factor structure among informal caregivers aligns with that of users and health workers. We have hypothesized that informal caregivers prioritize users' needs and rights over the care context's climate.MethodsThis was a cross-sectional study. The “Well-being at Work and Respect for Human Rights” questionnaire was distributed to 100 caregivers in 4 territorial mental health facilities in Sardinia, Italy. Confirmatory Factor Analysis (CFA) was utilized to assess the participants' responses.ResultsParticipants reported high satisfaction with their relatives' treatment, perceiving a high level of respect for human rights among users and healthcare professionals. However, they highlighted insufficient resources for services, particularly the need for additional staff. CFA revealed that a scale with the first five items demonstrated good reliability, convergent validity, and discrimination. Mean scores indicated high satisfaction and perception of respect for human rights across the sample, with no significant differences by age or gender.ConclusionSatisfaction with users' rights is closely correlated with other factors comprising the notion of organizational well-being within a healthcare service.