bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic differentiation and diversity of Callosobruchus chinensis collections from China
Authors: Duan C.X. , Li W.C. , Zhu Z.D. , Li D.D. , Sun S.L. , Wang X.M. 
Published: November 2015

AbstractCallosobruchus chinensis (Linnaeus) is one of the most destructive pests of leguminous seeds. Genetic differentiation and diversity analysis of 345 C. chinensis individuals from 23 geographic populations using 20 polymorphic simple sequence repeats revealed a total of 149 alleles with an average of 7.45 alleles per locus. The average Shannon's information index was 1.015. The gene flow and genetic differentiation rate values at the 20 loci ranged from 0.201 to 1.841 and 11.0–47.2%, with averages of 0.849 and 24.4%, respectively. In the 23 geographic populations, the effective number of alleles and observed heterozygosity ranged from 1.441 to 2.218 and 0.191–0.410, respectively. Shannon's information index ranged from 0.357 to 0.949, with the highest value in Hohhot and the lowest in Rudong. In all comparisons, the fixation index (FST) values ranged from 0.049 to 0.441 with a total FST value of 0.254 among the 23 C. chinensis populations, indicating a moderate level of genetic differentiation and gene flow among these populations. Analysis of molecular variance revealed that the genetic variation within populations accounted for 76.7% of the total genetic variation. The genetic similarity values between populations varied from 0.617 to 0.969, whereas genetic distances varied from 0.032 to 0.483. Using unweighted pair-group method using arithmetical averages cluster analysis, the 23 geographic collections were classified into four distinct genetic groups but most of them were clustered into a single group. The pattern of the three concentrated groups from polymerase chain reactions analysis showed a somewhat different result with cluster.
The effect of triterpenoid saponins fromSaponaria officinalison some blood hormones, metabolic parameters and fatty acid composition in dairy cows
Authors: SZKUDELSKA K. , SZUMACHER-STRABEL M. , SZCZECHOWIAK J. , BRYSZAK M. , PERS-KAMCZYC E. , STOCHMAL A. , CIESLAK A. 
Published: November 2015

SUMMARYAn experiment was performed to determine the effect of triterpenoid saponins from powdered root ofSaponaria officinalisL. (SO) on some blood metabolic parameters and hormone concentrations in dairy cows. Three dairy Polish Holstein-Friesian cows were used in the experiment in a 3 × 3 Latin Square arrangement. Animals were fed twice a day with a control diet and two experimental diets – a diet supplemented with either 440 or 660 g/d of the powderedS. officinalisroot (SO1 and SO2, respectively). Each of the three experimental cycles lasted for 26 days, including a 23-day adaptation period and a 3-day sample collection period. Blood samples were collected from the jugular vein from 24th to 26th day of the experiment. Total high-density lipoproteins cholesterol (HDL) and low-density lipoproteins cholesterol (LDL), as well as triglycerides, free fatty acids (FFA) and glucose, were assayed in serum. Fatty acid composition in the blood was also analysed. Moreover, concentrations of insulin, glucagon, leptin, triiodothyronine (T3) and thyroxine (T4) were measured. It was demonstrated that total and HDL-cholesterol concentrations were significantly elevated in the blood of cows treated with experimental diets; however, LDL-cholesterol remained unchanged. No concentrations of triglycerides, FFA or glucose were influenced by saponins. Two fatty acids (C16 : 0 and C16 : 1c9) were markedly reduced when SO was used, while C18 : 1t11decreased with increasing levels of SO. The significant increase of C20 : 4n-6in animals treated with SO was observed. Both saponin diets resulted in a slight increase in insulin concentration and the SO2 diet evoked an emphatic rise of glucagon concentration. The concentration of T3 also increased after consumption of the experimental diet. The current study shows for the first time that triterpenoid saponins fromS. officinaliscan alter blood parameters in ruminants. These effects seem to result from saponin-induced changes in the rumen.
Antarctic values and Malaysia's involvement in Antarctica: perceptions among young citizens of Malaysia
Authors: Ahmad Shabudin Ahmad Firdaus , Abdul Rahim Rashidah , Md Nor Norizan , Ibrahim Kamarulazizi 
Published: November 2015

ABSTRACTLack of support and interest among the public is one of the major challenges in strengthening and sustaining the future national agendas on Antarctica. The main goal of this study is to identify the perception of Malaysia's public with regard to Antarctic values and Malaysian involvement in the region. A survey on Malaysia's young citizen perception on Antarctic has been conducted for mapping out the future landscape of Malaysian involvement in the Antarctic region. Surveys have been conducted on respondents from secondary schools and universities in Malaysia. A questionnaire was designed to seek respondents’ knowledge and awareness on the values of Antarctica and their opinions on Malaysia's involvement. The results of the study showed that respondents had a high level of awareness of Antarctic environmental issues. However, general knowledge about the Antarctic region was still intermediate. On the other hand, the results of this study showed that the young citizens had positive and strong support for strengthening Malaysia's Antarctic involvement. This study hopes to contribute as baseline data on the matter. Such background information will reflect on the national strategy in strengthening Malaysia's current policy and future involvement in Antarctica.
Perception et réceptivité des proches-aidants à l’égard de la vidéosurveillance intelligente pour la détection des chutes des aînés à domicile
Authors: Lapierre Nolwenn , Proulx Goulet Chloë , St-Arnaud Alain , Ducharme Francine , Meunier Jean , Turgeon Londei Sophie , Saint-Arnaud Jocelyne , Giroux Francine , Rousseau Jacqueline 
Published: November 2015

ABSTRACTTo address the issue of falls, which are increasing as the population ages, an intelligent video-monitoring system is being developed. The aim of the study is to explore caregivers’ perceptions of and receptiveness to a prototype of this fall detection system. A cross-sectional mixed-method study was carried out with individual interviews of 18 caregivers. Statistical frequencies and content analysis were conducted (SPSS and N’Vivo). The results show that most participants (n = 15/18) liked the intelligent video-monitoring system and were willing to use it. They would worry less if they could be alerted if a care recipient fell, but they were concerned about privacy and cost. Participants had a positive perception of the system and expressed their wishes regarding the kind of alert and the person to contact in case of a fall.
Staphylococcus aureusbacteraemia associated with injected new psychoactive substances
Authors: GRIFFITH D. J. , MACKINTOSH C. L. , INVERARITY D. 
Published: November 2015

SUMMARYInjecting drug use is often associated with deep-seated infection. In Lothian in Scotland there has been a recent increase in the use of injected new psychoactive substances (NPS). Patients who have injected NPS have presented withStaphylococcus aureusbacteraemia (SAB) with life-threatening complications. We describe a unique case-series of 14 episodes of SAB in ten patients. Users of injected NPS had a significantly higher incidence of endocarditis and cavitating pulmonary lesions (P< 0·05) compared to those who inject only opiates. Cases of SAB in people who inject NPS have contributed to a significant rise in the overall incidence of SAB in people who inject drugs (P< 0·05) which has in turn impacted on the ability of Lothian to meet national targets for reducing the incidence of SAB.
Epidemiological analysis of pneumococcal serotype 19A in healthy children following PCV7 vaccination
Authors: TÓTHPÁL A. , LAUB K. , KARDOS S. , TIRCZKA T. , KOCSIS A. , VAN DER LINDEN M. , DOBAY O. 
Published: November 2015

SUMMARYAfter the introduction of conjugate vaccines, a strong rearrangement of pneumococcal serotypes was observed globally. Probably most concerning was the emergence of serotype 19A, which has not only high invasive disease potential, but also high antibiotic resistance. In the current study we focused on the increased prevalence of serotype 19A after the PCV vaccination rate became widely used in Hungary. A total of 2262 children aged 3–6 years were screened for pneumococcus carriage using nasal swabs. Children were divided into two groups according to the vaccination rates, low level (group 1) vs. high level (group 2). While the carriage rate did not change over time (average 32·9%), the serotype distribution differed greatly in the two groups. The prevalence of serotype 19A increased >eightfold. Almost all 19A isolates had high-level macrolide resistance and elevated penicillin minimum inhibitory concentrations. Genotyping methods revealed that these new 19A isolates are different from the previously frequent Hungary19A−6 PMEN clone. Both the carriage rate and the overall penicillin and macrolide resistance remained stable over time, but while several serotypes were represented in group 1, serotype 19A alone was clearly dominant in group 2.
Increased Delay Discounting on a Novel Real-Time Task among Girls, but not Boys, with ADHD
Authors: Rosch Keri S. , Mostofsky Stewart H. 
Published: November 2015

AbstractThe aim of this study was to examine delay discounting in girls and boys with ADHD-Combined type (ADHD-C) relative to typically developing (TD) children on two tasks that differ in the extent to which the rewards and delays were experienced by participants. Children ages 8–12 years with ADHD-C (n=65; 19 girls) and TD controls (n=55; 15 girls) completed two delay discounting tasks involving a series of choices between smaller, immediate and larger, delayed rewards. The classic delay discounting task involved choices about money at delays of 1–90 days and only some of the outcomes were actually experienced by the participants. The novel real-time discounting task involved choices about an immediately consumable reward (playing a preferred game) at delays of 25–100 s, all of which were actually experienced by participants. Participants also provided subjective ratings of how much they liked playing the game and waiting to play. Girls with ADHD-C displayed greater delay discounting compared to boys with ADHD-C and TD girls and boys on the real-time discounting task. Diagnostic group differences were not evident on the classic discounting task. In addition, children with ADHD-C reported wanting to play the game more and liking waiting to play the game less than TD children. This novel demonstration of greater delay discounting among girls with ADHD-C on a discounting task in which the rewards are immediately consumable and the delays are experienced in real-time informs our understanding of sex differences and motivational processes in children with ADHD. (JINS, 2016, 22, 12–23)
A Meshless Algorithm to Model Field Evaporation in Atom Probe Tomography
Authors: Rolland Nicolas , Vurpillot François , Duguay Sébastien , Blavette Didier 
Published: November 2015

AbstractAn alternative approach for simulating the field evaporation process in atom probe tomography is presented. The model uses the electrostatic Robin’s equation to directly calculate charge distribution over the tip apex conducting surface, without the need for a supporting mesh. The partial ionization state of the surface atoms is at the core of the method. Indeed, each surface atom is considered as a point charge, which is representative of its evaporation probability. The computational efficiency is ensured by an adapted version of the Barnes–Hut N-body problem algorithm. Standard desorption maps for cubic structures are presented in order to demonstrate the effectiveness of the method.
Gingival fibroblasts resist apoptosis in response to oxidative stress in a model of periodontal diseases
Authors: Cheng R , Choudhury D , Liu C , Billet S , Hu T , Bhowmick NA 
Published: November 2015

AbstractPeriodontal diseases are classified as inflammation affecting the supporting tissue of teeth, which eventually leads to tooth loss. Mild reversible gingivitis and severe irreversible periodontitis are the most common periodontal diseases. Periodontal pathogens initiate the diseases. The bacterial toxin, lipopolysaccharide (LPS), triggers the inflammatory response and leads to oxidative stress. However, the progress of oxidative stress in periodontal diseases is unknown. The purpose of this study is to examine oxidative stress and cell damage in gingivitis and periodontitis. Our results showed that LPS increases reactive oxygen species (ROS) accumulation in gingival fibroblast (GF). However, oxidative stress resulting from excessive ROS did not influence DNA damage and cell apoptosis within 24 h. The mechanism may be related to the increased expression of DNA repair genes, Ogg1, Neil1 and Rad50. Detection of apoptosis-related proteins also showed anti-apoptotic effects and pro-apoptotic effects were balanced. The earliest damage appeared in DNA when increased γH2AX, an early biomarker for DNA damage, was detected in the LPS group after 48 h. Later, when recurrent inflammation persisted, 8-OHdG, a biomarker for oxidative stress was much higher in periodontitis model compared to the control in vivo. Staining of 8-OHdG in human periodontitis specimens confirmed the results. Furthermore, TUNEL staining of apoptotic cells indicated that the periodontitis model induced more cell apoptosis in gingival tissue. This suggested GF could resist early and acute inflammation (gingivitis), which was regarded as reversible, but recurrent and chronic inflammation (periodontitis) led to permanent cell damage and death.
Cisplatin-induced apoptosis in auditory, renal, and neuronal cells is associated with nitration and downregulation of LMO4
Authors: Rathinam R , Ghosh S , Neumann WL , Jamesdaniel S 
Published: November 2015

AbstractCytotoxic effects of cisplatin occur primarily through apoptosis. Though several pro- and anti-apoptotic signaling molecules have been identified to play an important role in mediating the ototoxic, nephrotoxic, and neurotoxic side effects of cisplatin, the underlying mechanism is yet to be fully characterized. We reported that nitration of LIM domain-only 4 (LMO4), a transcriptional regulator, facilitates cochlear apoptosis in cisplatin-induced ototoxicity. However, its role in cisplatin-mediated nephrotoxicity and neurotoxicity is poorly understood. Therefore, HK2 and SH-SY5Y cells were used along with UBOC1 cells, to investigate the perturbations of LMO4 in cisplatin-induced cytotoxicity, in renal, neuronal, and auditory cells, respectively. Cisplatin induced an increase in the expression of active caspase-3, indicating cellular apoptosis, and increased the nitration of proteins, 24 h post treatment. Immunostaining with anti-nitrotyrosine and anti-LMO4 indicated that nitrotyrosine co-localized with LMO4 protein in cisplatin-treated cells. Immunoblotting with anti-LMO4 indicated that cisplatin induced a decrease in LMO4 protein levels. However, a corresponding decrease in LMO4 gene levels was not observed. Inhibition of protein nitration with SRI110, a peroxynitrite decomposition catalyst, attenuated cisplatin-induced downregulation of LMO4. More importantly, overexpression of LMO4 mitigated the cytotoxic effects of cisplatin in UBOC1 cells while a dose-dependent decrease in LMO4 protein strongly correlated with cell viability in UBOC1, HK2, and SH-SY5Y cells. Collectively, these findings suggested a potential role of LMO4 in facilitating the cytotoxic effects of cisplatin in auditory, renal, and neuronal cells.
Planar-integrated single-crystalline perovskite photodetectors
Authors: Saidaminov Makhsud I. , Adinolfi Valerio , Comin Riccardo , Abdelhady Ahmed L. , Peng Wei , Dursun Ibrahim , Yuan Mingjian , Hoogland Sjoerd , Sargent Edward H. , Bakr Osman M. 
Published: November 2015

AbstractHybrid perovskites are promising semiconductors for optoelectronic applications. However, they suffer from morphological disorder that limits their optoelectronic properties and, ultimately, device performance. Recently, perovskite single crystals have been shown to overcome this problem and exhibit impressive improvements: low trap density, low intrinsic carrier concentration, high mobility, and long diffusion length that outperform perovskite-based thin films. These characteristics make the material ideal for realizing photodetection that is simultaneously fast and sensitive; unfortunately, these macroscopic single crystals cannot be grown on a planar substrate, curtailing their potential for optoelectronic integration. Here we produce large-area planar-integrated films made up of large perovskite single crystals. These crystalline films exhibit mobility and diffusion length comparable with those of single crystals. Using this technique, we produced a high-performance light detector showing high gain (above 104 electrons per photon) and high gain-bandwidth product (above 108 Hz) relative to other perovskite-based optical sensors.
Enhancing the linear flow of fine granules through the addition of elongated particles
Authors: Guo Zhiguo , Chen Xueli , Xu Yang , Liu Haifeng 
Published: November 2015

AbstractSandglasses have been used to record time for thousands of years because of their constant flow rates; however, they now are drawing attention for their substantial scientific importance and extensive industrial applications. The presence of elongated particles in a binary granular system is believed to result in undesired flow because their shape implies a larger resistance to flow. However, our experiments demonstrate that the addition of elongated particles can substantially reduce the flow fluctuation of fine granules and produce a stable linear flow similar to that in an hourglass. On the basis of experimental data and previous reports of flow dynamics, we observed that the linear flow is driven by the “needle particle effect,” including flow orientation, reduced agglomeration and local perturbation. This phenomenon is observed in several binary granular systems, including fine granules and secondary elongated particles, which demonstrates that our simple method can be widely applied to the accurate measurement of granular flows in industry.
The role of H4 flagella in Escherichia coli ST131 virulence
Authors: Kakkanat Asha , Totsika Makrina , Schaale Kolja , Duell Benjamin L. , Lo Alvin W. , Phan Minh-Duy , Moriel Danilo G. , Beatson Scott A. , Sweet Matthew J. , Ulett Glen C. , Schembri Mark A. 
Published: November 2015

AbstractEscherichia coli sequence type 131 (ST131) is a globally dominant multidrug resistant clone associated with urinary tract and bloodstream infections. Most ST131 strains exhibit resistance to multiple antibiotics and cause infections associated with limited treatment options. The largest sub-clonal ST131 lineage is resistant to fluoroquinolones, contains the type 1 fimbriae fimH30 allele and expresses an H4 flagella antigen. Flagella are motility organelles that contribute to UPEC colonisation of the upper urinary tract. In this study, we examined the specific role of H4 flagella in ST131 motility and interaction with host epithelial and immune cells. We show that the majority of H4-positive ST131 strains are motile and are enriched for flagella expression during static pellicle growth. We also tested the role of H4 flagella in ST131 through the construction of specific mutants, over-expression strains and isogenic mutants that expressed alternative H1 and H7 flagellar subtypes. Overall, our results revealed that H4, H1 and H7 flagella possess conserved phenotypes with regards to motility, epithelial cell adhesion, invasion and uptake by macrophages. In contrast, H4 flagella trigger enhanced induction of the anti-inflammatory cytokine IL-10 compared to H1 and H7 flagella, a property that may contribute to ST131 fitness in the urinary tract.
Tears of wine: new insights on an old phenomenon
Authors: Venerus David C. , Nieto Simavilla David 
Published: November 2015

AbstractAnyone who has enjoyed a glass of wine has undoubtedly noticed the regular pattern of liquid beads that fall along the inside of the glass, or ‘tears of wine.’ The phenomenon is the result of a flow against gravity along the liquid film on the glass, which is induced by an interfacial tension gradient. It is generally accepted that the interfacial tension gradient is due to a composition gradient resulting from the evaporation of ethanol. We re-examine the tears of wine phenomenon and investigate the importance of thermal effects, which previously have been ignored. Using a novel experiment and simple model we find that evaporative cooling contributes significantly to the flow responsible for wine tears and that this phenomenon occurs primarily because of the thermodynamic behavior of ethanol-water mixtures. Also, the regular pattern of tear formation is identified as a well-known hydrodynamic instability.
Investigation of the fatty acid transporter-encoding genes SLC27A3 and SLC27A4 in autism
Authors: Maekawa Motoko , Iwayama Yoshimi , Ohnishi Tetsuo , Toyoshima Manabu , Shimamoto Chie , Hisano Yasuko , Toyota Tomoko , Balan Shabeesh , Matsuzaki Hideo , Iwata Yasuhide , Takagai Shu , Yamada Kohei , Ota Motonori , Fukuchi Satoshi , Okada Yohei , Akamatsu Wado , Tsujii Masatsugu , Kojima Nobuhiko , Owada Yuji , Okano Hideyuki , Mori Norio , Yoshikawa Takeo 
Published: November 2015

AbstractThe solute carrier 27A (SLC27A) gene family encodes fatty acid transport proteins (FATPs) and includes 6 members. During fetal and postnatal periods of development, the growing brain requires a reliable supply of fatty acids. Because autism spectrum disorders (ASD) are now recognized as disorders caused by impaired early brain development, it is possible that functional abnormalities ofSLC27Agenes may contribute to the pathogenesis of ASD. Here, we confirmed the expression ofSLC27A3andSLC27A4in human neural stem cells derived from human induced pluripotent stem cells, which suggested their involvement in the developmental stage of the central nervous system. Additionally, we resequenced theSLC27A3andSLC27A4genes using 267 ASD patient and 1140 control samples and detected 47 (44 novel and 29 nonsynonymous) and 30 (17 novel and 14 nonsynonymous) variants for theSLC27A3andSLC27A4, respectively, revealing that they are highly polymorphic with multiple rare variants. The SLC27A4 Ser209 allele was more frequently represented in ASD samples. Furthermore, we showed that a SLC27A4 Ser209 mutant resulted in significantly higher fluorescently-labeled fatty acid uptake into bEnd3 cells, a mouse brain capillary-derived endothelial cell line, compared with SLC27A4 Gly209, suggesting that the functional change may contribute to ASD pathophysiology.