bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulatory variation at serotonin receptor 1F (HTR1F) modulates arousals and risk for sleep apnea
Authors: Strausz Satu , Broberg Martin , Jones Samuel E , Koskela Jukka , Kiiskinen Tuomo , FinnGen  , Palotie Aarno , Palotie Tuula , Bachour Adel , Saxena Richa , Ripatti Samuli , Abner Erik , Ollila Hanna M. 
Published: August 2024

AbstractBackgroundSleep apnea is a common sleep disorder affecting at least ten percent of the population. It is caused by lack of breathing during sleep, typically mediated by obstruction of airways or less frequently by misdirected central signals for breathing. The primary risk factor is a high body mass index (BMI), causing airway obstruction. However, understanding risk factors for sleep apnea in non-obese (BMI < 30) individuals requires further exploration.AimOur goal was to elucidate genetic risk factors for sleep apnea in non-obese individuals.MethodsWe performed genome-wide association testing in individuals with BMI < 30 in FinnGen including 20,413 cases with sleep apnea diagnosis (ICD-10 G47.3 or ICD-9 3472) and 443,463 disease free controls. We replicated our analysis in Estonian Biobank.ResultsWe identified a significant association within the Serotonin receptor 1F (HTR1F) locus (rs1818163, beta = 0.059, se = 0.010, P < 1.58e-8), and replicated the association in Estonian Biobank (beta =0.042, se = 0.021, P = 0.046). The association signal co-localized withHTR1Fexpression across multiple tissues (posterior probability > 0.8), and single cell sequencing implicatedHTR1Fexpression particularly in neurons. Analysis of eQTL data further supported a possible regulatory role in neurons (beta = -0.03, P = 1.2e-4). Finally, objectively measured sleep-activity data showed association with number awakenings during night (P = 5.6e-8).ConclusionsThe findings indicate association ofHTR1Fin sleep apnea particularly in the patient population within the non-obese BMI range and provide insight into the growing evidence of serotonin signaling as a factor modulating liability to sleep apnea.
Functional divergence of conserved developmental plasticity genes between two distantly related nematodes
Authors: Wighard Sara , Witte Hanh , Sommer Ralf J. 
Published: August 2024

AbstractGenes diverge in form and function in multiple ways over time; they can be conserved, acquire new roles, or eventually be lost. However, the way genes diverge at the functional level is little understood, particularly in plastic systems. We investigated this process using two distantly related nematode species,Allodiplogaster sudhausiandPristionchus pacificus. Both these nematodes display environmentally-influenced developmental plasticity of mouth-form feeding structures. This phenotype can be manipulated by growth on particular diets, making them ideal traits to investigate functional divergence of developmental plasticity genes between organisms. Using CRISPR-engineered mutations inA. sudhausimouth-form genes, we demonstrate examples of the various ways ancestral genes regulate developmental plasticity and how these roles can progressively diverge. We examined four ancestral genes, revealing distinct differences in their conservation and functional divergence in regulating the mouth phenotype in both species. Specifically, certain genes retain the same characteristics, while others have acquired a new function. Additionally, two ancestral genes retain their functions as switch genes, which completely prevent a phenotype, and the other two display quantitative effects, with knockouts in these genes displaying intermediate phenotypes. Remarkably, despite the evolutionary distance, all genes examined were involved in mouth-form regulation. Finally, multiple gene knock-out mutants were engineered, with key sulfatase-encoding genes acting downstream of all others, suggesting they play a major role in mouth-form plasticity. Together, this study represents the first mutant-based functional analysis of the evolution of developmental plasticity between two highly diverged species, offering new insights into the genetic mechanisms underlying phenotypic evolution.Article SummaryWhile evolutionary divergence of genes is well-studied at the sequence level, the resulting functional and phenotypic consequences are less known, particularly in plastic systems. Here, we examined functional divergence of a set of genes involved in developmental plasticity of mouth-form between two highly diverged nematode species. We found that all studied genes control mouth-form plasticity in both species; however, with strong functional divergence and gene-specific quantitative effects or even novel functions. Thus, there is a spectrum from full conservation, partial conservation to the gain of a new function; with genes involved in sulfation showing the strongest conservation during evolution.
CRISPR/dCas-mediated counter-silencing – Reprogramming dCas proteins into antagonists of xenogeneic silencers
Authors: Wiechert Johanna , Roigé Biel Badia , Dohmen-Olma Doris , Hindra  , Zhang Xiafei , Stella Roberto G. , Elliot Marie A. , Frunzke Julia 
Published: August 2024

ABSTRACTLsr2-like nucleoid-associated proteins function as xenogeneic silencers (XSs) inhibiting expression of horizontally acquired, AT-rich DNA in actinobacteria. Interference by transcription factors can lead to counter-silencing of XS target promoters, but relief of this repression typically requires promoter engineering. In this study, we developed a novel CRISPR/dCas-mediated counter-silencing (CRISPRcosi) approach by using nuclease-deficient dCas enzymes to counteract the Lsr2-like XS protein CgpS inCorynebacterium glutamicumor Lsr2 inStreptomyces venezuelae. Systematicin vivoreporter studies with dCas9 and dCas12a and various guide RNAs revealed effective counter-silencing of different CgpS target promoters in response to guide RNA/dCas DNA binding – independent of promoter sequence modifications. The most prominent CRISPRcosi effect was observed when targeting the CgpS nucleation site, an effect that was also seen inS. venezuelaewhen targeting a known Lsr2 nucleation site within the chloramphenicol biosynthesis gene cluster. Analyzing the system inC. glutamicumstrains lacking the XS protein CgpS revealed varying strengths of counteracting CRISPR interference (CRISPRi) effects based on the target position and strand. Genome-wide transcriptome profiling in sgRNA/dCas9 co-expressingC. glutamicumwild-type strains revealed high counter-silencing specificity with minimal off-target effects. Thus, CRISPRcosi provides a promising strategy for the precise upregulation of XS target genes with significant potential for studying gene networks as well as for developing applications in biotechnology and synthetic biology.IMPORTANCELsr2-like nucleoid-associated proteins act as xenogeneic silencers (XSs), repressing the expression of horizontally acquired, AT-rich DNA in actinobacteria. The targets of Lsr2-like proteins are very diverse, including prophage elements, virulence gene clusters and biosynthetic gene clusters. Consequently, the targeted activation of XS target genes is of interest for fundamental research and biotechnological applications. Traditional methods for counter-silencing typically require promoter modifications. In this study, we developed a novel CRISPR/dCas-mediated counter-silencing (CRISPRcosi) approach, utilizing nuclease-deficient dCas enzymes to counteract repression by Lsr2-like proteins inCorynebacterium glutamicumandStreptomyces venezuelae. The strongest effect was observed when targeting the Lsr2 nucleation site. Genome-wide transcriptome profiling revealed high specificity with minimal off-target effects. Overall, CRISPRcosi emerges as a powerful tool for the precise activation of genes silenced by xenogeneic silencers, offering new opportunities for exploring gene networks and advancing biotechnological applications.
Does carbon performance payoff? An empirical evidence from Asia-Pacific region
Authors: Jangid Mohan Lal , Sharma Anil Kumar 
Published: August 2024

PurposeThis study primarily examines the link between carbon and financial performance in the Asia-Pacific region. In addition, the study also explores how the economic impact of carbon performance varies in carbon-intensive and non-carbon-intensive industries.Design/methodology/approachThis study takes a sample of 1,539 non-financial firms from 13 Asia-Pacific countries from 2014 to 2021. It employs a firm-fixed effect panel regression model to examine the objective.FindingsThe findings indicate that carbon performance improvement enhances accounting-based and market-based financial performance. The positive impact of carbon abatement stems from increased operational efficiency, energy efficiency and lower production costs. Further, the stock market participants also reward the firm for carbon efficiency. However, the carbon intensity of industrial sectors presents a conflicting picture for this association.Originality/valueThis study adds insights to the literature by providing a contemporary reflection on the nexus between carbon emissions and economic outcomes in the understudied Asia-Pacific region. It also unveils the nuanced difference in the carbon-financial performance relationship attributed to industries' carbon sensitivity.
The planning of Greater Amman: migrants to migrant capital
Authors: Abu-Dayyeh Nabil 
Published: August 2024

PurposeClose inspection of some of the more intricate details of the two most recent planning efforts, the award-winning Amman Plan 2025 and the strategic master plan known as the Amman Development Corridor Study (ADC), particularly in their most direct area of overlap, that is, the Metropolitan Growth Strategy.Design/methodology/approachStudy and interpretation of published documents relevant to the plans in question.FindingsThe study reveals that the emerging objectives of accommodating migrant capital within the context of state-wide neo-liberal restructuring, particularly at the city’s eastern and south-eastern edge, have yet to benefit from recent scholarship on productive suburbanization.Research limitations/implicationsLack of data on Foreign Direct Investment in Amman.Practical implicationsThe results have implications for the future urban growth scenario of Greater Amman.Social implicationsInformal (illegal) building on the fringes of the city continues unabated. It is encouraged by permissive planning practice, a long-standing aspect of local practice dating to the 1970s. The longer that planning action lags, particularly at the eastern fringes, the more intense will be the informal building, and the higher the prospects for social conflict.Originality/valueThere has been only one, rather uncritical, published research on the Amman Plan, but none so far discussing the ADC study and its proposals.
Exploring the impacts of leader anger expressions on employee boundary-spanning behavior in construction projects
Authors: Kang Fei , Shi Yifei , Li Jiyu , Zhang Han 
Published: August 2024

PurposeDespite the increasing importance of boundary-spanning behaviors in construction projects, the research on how leader anger expressions impact employees’ boundary-spanning behaviors remains unclear. This study aims to investigate the impact of leader anger expressions on employees’ boundary-spanning behaviors in construction projects while exploring the mediating effect of work hope and the moderating effect of power distance orientation through the lens of social information processing theory.Design/methodology/approachThe empirical data were collected from a questionnaire survey of 235 employees in construction projects, and the hypotheses were tested using the PROCESS program developed by Hayes.FindingsThis study demonstrated that leader anger expressions negatively affect employees’ work hope, and work hope mediates the relationship between leader anger expressions and employees’ boundary-spanning behaviors. Besides, the stronger the employees’ power distance orientation, the stronger the negative relationship between leader anger expressions and employees’ work hope, and the above mediating effect was also stronger.Research limitations/implicationsThe use of cross-sectional data was unable to entirely infer a causal relationship between leader anger expressions and employees’ boundary-spanning behaviors. Additionally, the reliance on self-reported data inevitably introduced some common method bias.Originality/valueApplying social information processing theory, this paper enriches the discourse on factors influencing employees’ boundary-spanning behaviors in construction projects. It also offers valuable insights for leaders in construction projects to manage their teams effectively.
Strategizing green marketing in times of uncertainty: does it pay off?
Authors: Papadas Karolos A. , Piha Lamprini , Davvetas Vasileios , Leonidou Constantinos N. 
Published: August 2024

Purpose
This study aims to investigate the impact of green marketing strategy (GMS) and firms’ decision to invest in or divest from green marketing activities during a crisis on business performance.

Design/methodology/approach
The study collected survey data from 245 Greek firms during the 2015 Eurozone crisis to investigate the impact of GMS and green marketing investments on firm resilience during crisis. Time-lagged, objective performance data for a subset of these firms helped examine the impact of GMS on postcrisis financial performance.

Findings
Pursuing a GMS builds resilience, especially for companies that decided not to reduce resources allocated to green marketing activities during a recession. Beyond resilience, firms investing in GMS during the crisis experienced improved financial performance in the long run. Finally, this research proposes a typology of GMS responses during a crisis.

Research limitations/implications
This study does not specify which types of green marketing activities lead to more investment or divestment during a crisis.

Practical implications
The study offers insights for allocating resources to green marketing during recessions. Supporting GMSs during unpredictable times is important to successfully navigate performance both during and after a crisis. Six crisis response profiles are offered: green-nonbelievers, dis-investors, reluctants and cautious-, opportunistic- and strategic-green investors.

Social implications
The study proposes a balanced approach to environmental sustainability, marketing strategy and firm performance during a crisis.

Originality/value
The study argues that GMSs enable firms to survive a crisis and recover from financial shocks.
An integrated numerical and analytical investigation on cilia-generated MHD flow of Jeffrey fluid through a porous medium
Authors: Zeeshan A. , Javed Hamza , Shehzad N. , Sait Sadiq M. , Ellahi R. 
Published: August 2024

Purpose
This study aims to examine the cilia-driven flow of magnetohydrodynamics (MHD) non-Newtonian fluid through a porous medium. The Jeffrey fluid model is taken into account. The fluid motion in a two-dimensional symmetric channel emphasizes the dominance of viscous properties over inertial properties in the context of long wavelength and low Reynolds number approximations.

Design/methodology/approach
An integrated numerical and analytic results are obtained by hybrid approach. A statistical method analysis of variance along with response surface methodology is used. Sensitivity analysis is used to validate the accuracy of nondimensional numbers.

Findings
The impact of various flow parameters is presented graphically and in numerical tables. It is noted that the velocity slip parameter is the most sensitive flow parameter in velocity and relaxation to retardation time ratio in temperature.

Originality/value
A model on cilia-generated flow of MHD non-Newtonian Jeffrey fluid is proposed.
Self-efficacy of school principals for effective school functioning during the COVID-19 crisis
Authors: Qadach Mowafaq 
Published: August 2024

PurposeThe current study seeks first to examine the prediction of school functioning in crises during the COVID-19 pandemic by school principals’ self-efficacy; second, to explore the differences in all dimensions of self-efficacy and school functioning during crises in Arab and Jewish schools in Israel and third, to determine which of school principals’ self-efficacy dimensions best predicted school functioning during the COVID-19 crisis.Design/methodology/approachData were aggregated at the school level for structural equation modeling (SEM) analysis using AMOS analysis of 103 middle schools across Israel, 53 from Jewish and 50 from Arab society. Participants included 103 school principals (who answered the school principals’ self-efficacy questionnaire) and 1,031 teachers who answered the school functioning during crises questionnaire (477 Jewish teachers and 554 Arab teachers).FindingsThe findings showed that the principals’ self-efficacy positively predicted school functioning during the crisis. Among the five self-efficacy dimensions (general management efficacy, leadership efficacy, human relations efficacy, efficacy in managing external relations and pedagogical management efficacy), significant differences were found only in “external relations efficacy,” which was higher for “Arab” school principals; the only dimension that predicted school functioning during crises in both societies was “human relations efficacy.”Originality/valueThe current results emphasize the importance of principals’ self-efficacy in general and specifically caring leadership practices “human relations efficacy” in their relations with the school staff, the students and the parents for effective school coping and functioning during crises in two societies in Israel: Arab and Jewish. Further, no previous studies have explored this correlation.
Shared high-performance work system perceptions as a competitive advantage: mediating role of trust in management in the HPWS-performance link
Authors: Pak Sunjin , Ju Boreum (Jenny) 
Published: August 2024

Purpose
This study aims to investigate the mediating role of trust in management and the moderating role of employee-management congruence in high-performance work system (HPWS) perceptions on the relationship between HPWS and firm performance.

Design/methodology/approach
Survey data on HPWS practices and employee–manager perceptions from a large sample of South Korean firms were integrated with objective financial performance data. Path analysis using STATA 18.0 with robust standard errors was used to test the hypothesised moderated mediation model.

Findings
Trust in management partially mediated the relationship between HPWS and firm performance. While employee–management congruence in HPWS perceptions did not moderate the direct effect of HPWS on firm performance, it significantly moderated the indirect effect through trust in management. The positive influence of HPWS on performance via trust was stronger when employee–management congruence was high.

Originality/value
This study extends the social exchange perspective on the HPWS–performance relationship by incorporating trust in management as a critical mediator and employee–management congruence in HPWS perceptions as a moderator. The findings highlight the importance of fostering shared understandings of human resource practices between employees and managers to optimise the trust-building and performance-enhancing effects of HPWS.
Managing supply chain risk through inter-organisational justice
Authors: Khamaiseh Odai , Alghababsheh Mohammad , Lekhavat Saowanit , Rahman Mushfiqur 
Published: August 2024

PurposeThis study examines the impact of inter-organisational justice (i.e. distributive, procedural and interactional) in the buyer–supplier relationship on supply risk and, in turn, on a firm’s marketing and financial performance.Design/methodology/approachA structured survey was administered both online and in-person to Jordan-based manufacturing companies. The 137 responses received were analysed using partial least structural equation modelling.FindingsThe study found that while establishing both procedural and interactional justice in the relationship has a negative impact on supply risk, promoting distributive justice, surprisingly, has no impact. Moreover, supply risk was found to be detrimental to the firm’s marketing and financial performance.Research limitations/implicationsThis study considers only the direct role of inter-organisational justice in reducing supply risk. Future research could enhance our understanding of this role by exploring the underlying mechanisms and conditions that could govern it.Practical implicationsManagers can alleviate supply risk by ensuring procedural and interactional justice in the relationship through involving suppliers in the decision-making processes, consistently adhering to established procedures and communicating transparent and ample information.Social implicationsAddressing supply risk can help in maintaining community resilience and economic stability.Originality/valueThe study highlights inter-organisational justice as a new approach to mitigating supply risk. Moreover, by examining how supply risk can affect a firm’s marketing performance, it also highlights a new implication of supply risk. Furthermore, by exclusively examining the impact of supply risk on a firm’s financial performance, the study provides a more nuanced interpretation of the effect of supply risk and how it can be reduced.
Managing consumer trust in e-commerce: evidence from advanced versus emerging markets
Authors: Quintus Michaela , Mayr Kathrin , Hofer Katharina Maria , Chiu Yen Ting 
Published: August 2024

PurposeGaining and maintaining trust in e-commerce is crucial for online purchases. Specifically, understanding trust formation and its consequences in a cross-market online shopping context is important, as cross-market studies are scarce. Therefore, this study examines antecedents and consequences of consumer trust in online shopping (TOS) by comparing advanced and emerging markets.Design/methodology/approachTo test the formulated hypotheses, data including 397 responses from Austria and 205 from Moldova are analysed. Using partial least squares (PLS) path modelling, implications for theory and practice in cross-market e-commerce are obtained.FindingsEmpirical findings show that company reputation, perceived security and website quality positively influence consumer TOS. TOS corresponds directly positively with purchase intentions (PI). Our research confirms the negative relationship between trust and perceived risk (PR) as well as that between PR and PI. Furthermore, a significant difference between Austria and Moldova regarding the influence of experience and perceived website quality (PWQ) on TOS is observed.Originality/valueOur study fills research gaps concerning TOS within the context of cross-market e-commerce. It contributes theoretically and practically and reveals the importance of customer trust and risk reduction for online retailers within advanced and emerging markets in order to provoke online PI.