bluuebunny/crossref_metadata_2025_split huggingface dataset:
Female oviposition decisions are influenced by the microbial environment
Authors: Fowler Emily K. , Friend Lucy A. , Churchill Emily R. , Yu Douglas W. , Archetti Marco , Bourke Andrew F.G. , Bretman Amanda , Chapman Tracey 
Published: July 2024

AbstractIn ovipositing animals, egg placement decisions can be key determinants of offspring survival. One oviposition strategy reported across taxa is egg clustering, whereby a female lays multiple eggs next to one another or next to the eggs of other females. The fitness benefits of egg clustering, especially in mixed maternity clusters, are unknown. In some species, mothers provision eggs with diffusible defence compounds, such as antimicrobials, raising the possibility of public good benefits arising from egg clustering. Here we report thatDrosophila melanogasterfemales frequently lay eggs in mixed maternity clusters. We tested two hypotheses for potential drivers of this oviposition behaviour: (i) the microbial environment affects fecundity and egg placement in groups ofD. melanogasterfemales; (ii)D. melanogastereggs exhibit antimicrobial activity. The results partially supported the first hypothesis. Females exposed to environmental microbes that naturally colonised the oviposition substrates in the absence of antimicrobial preservatives reduced their levels of fecundity but did not significantly alter egg clustering. In contrast, the presence of commensal (fly-associated) microbes did not affect oviposition. The second hypothesis was not supported. There was no evidence of antimicrobial activity, either in whole eggs or in soluble surface material extracted from them. In conclusion, while there was no evidence that oviposition decisions are shaped by the opportunity to share antimicrobials, there is evidence that the microbial environment provides cues that females use to make sophisticated decisions on fecundity and egg placement.
Pathophysiology of hypereosinophilia-associated heart disease
Authors: Sunusi Usman , Ziegelmeyer Ben , Osuji Immaculeta , Medvedovic Mario , Todd Haley , Abou-Khalil Joe , Zimmermann Nives 
Published: July 2024

AbstractBackgroundCardiac complications in patients with hypereosinophilia cause significant morbidity and mortality. However, mechanisms of how eosinophilic inflammation causes heart damage are poorly understood.MethodsWe developed a model of hypereosinophilia-associated heart disease by challenging hypereosinophilic mice with peptide from the cardiac myosin heavy chain. Disease outcomes were measured by histology, immunohistochemistry, flow cytometry, and measurement of cells and biomarkers in peripheral blood. Eosinophil dependence was determined by using eosinophil-deficient mice (ΔdblGATA). Single cells from heart were subjected to single cell RNA sequencing to assess cell composition, subtypes and expression profiles.ResultsMice challenged with myocarditic and control peptide had peripheral blood leukocytosis, but only those challenged with myocarditic peptide had heart inflammation. Heart tissue was infiltrated by eosinophil-rich inflammatory infiltrates associated with cardiomyocyte damage. Disease penetrance and severity were dependent on the presence of eosinophils. Single cell RNA sequencing showed enrichment of myeloid cells, T-cells and granulocytes (neutrophils and eosinophils) in the myocarditic mice. Macrophages were M2 skewed, and eosinophils had an activated phenotype. Gene enrichment analysis identified several pathways potentially involved in pathophysiology of disease.ConclusionEosinophils are required for heart damage in hypereosinophilia-associated heart disease. Additionally, myeloid cells, granulocytes and T-cell cooperatively or independently participate in the pathogenesis of hypereosinophilia-associated heart disease.
Niche-Based Priority Effects Predict Microbe Resistance toErwinia amylovorain Pear Nectar
Authors: McDaniel Christopher S. , Vannette Rachel L. , Arroyo-Flores Alondra , Boundy-Mills Kyria , Crowder David W. , Grilley Michelle M. , Pathak Honey , Schaeffer Robert N. 
Published: July 2024

ABSTRACTFire blight is a devastating disease affecting pome fruit trees that is caused byErwinia amylovoraand leads to substantial annual losses worldwide. While antibiotic-based management approaches like streptomycin can be effective, there are concerns over evolved resistance of the pathogen and non-target effects on beneficial microbes and insects. Using microbial biological control agents (mBCAs) to combat fire blight has promise, but variable performance necessitates the discovery of more effective solutions. Here we used a niche-based predictive framework to assess the strength of priority effects exerted by prospective mBCAs, and the mechanisms behind growth suppression in floral nectar. Throughin vitroandin vivoassays, we show that antagonist impacts on nectar pH and sucrose concentration were the primary predictors of priority effects. Surprisingly, overlap in amino acid use, and the degree of phylogenetic relatedness between mBCA andErwiniadid not significantly predict pathogen suppressionin vitro, suggesting that competition for limited shared resources played a lesser role than alterations in the chemical environment created by the initial colonizing species. We also failed to detect an association between our measures ofin vitroandin vivo Erwiniasuppression, suggesting other mechanisms may dictate mBCA establishment and efficacy in flowers, including priming of host defenses.
SARS-CoV-2 vaccination during pregnancy enhances hippocampal neurogenesis and working memory in offspring via IFN-gamma responsive microglia
Authors: Liu Fangfang , Tang Jiaoling , Zou Juntao , Liu Hao , Zuo Zejie , Wang Lingxiao , Wang Na , Li Zhihui , Kumar Ashutosh , Guo Kaihua , Hu Dandan , Yao Zhibin 
Published: July 2024

AbstractIn the face of severe adverse outcomes of pandemic Coronavirus disease 2019 (COVID-19) infection, vaccines proved potently immunogenic and safe in humans and are today strongly recommended in pregnancy. This study investigates, in offspring mice, the effect of maternal COVID-19 vaccination on postnatal physical development, behavior and neurogenesis. After inoculation with inactivated COVID-19 vaccine (Vero Cell) at gestational day 14.5, antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) were detected in serum of both dams and pups. At one month of age, pups born to vaccinated dams, but not the offspring of non-vaccinated dams, exhibited greater working memory and more neural cell proliferation, neuroblast formation, neuronal stem cell activity and larger numbers of mature neurons within the dentate gyrus (DG). Luminex multiplex assay revealed elevated levels of hippocampal cytokines/chemokines critical to neurogenesis and memory function, namely interferon-γ [IFN-γ] and CX3C motif chemokine ligand 1 [CX3CL1]. Conditional knockout technology implicated microglial IFN-γ receptor 1 (IFNγR1) and CX3C motif chemokine receptor 1 (CX3CR1) as crucial intercellular participants in the neuronal developmental process, via regulating microglial activation and chemotaxis, respectively. We propose that, rather than posing risk for neurodevelopmental abnormalities, maternal SARS-CoV-2 vaccination transiently enhances hippocampal neurogenesis and working memory in offspring.
Structural models predict a significantly higher binding affinity between the NblA protein of cyanophage Ma-LMM01 and the phycocyanin ofMicrocystis aeruginosaNIES-298 compared to the host homolog
Authors: Meza-Padilla Isaac , McConkey Brendan J. , Nissimov Jozef I. 
Published: July 2024

AbstractHorizontal gene transfer events between viruses and hosts are widespread across the virosphere. In cyanophage-host systems, such events often involve the transfer of genes involved in photosynthetic processes. The genome of the lytic cyanomyovirus Ma-LMM01 infecting the toxic, bloom-forming, freshwaterMicrocystis aeruginosaNIES-298 contains a homolog of thenon-bleaching A(nblA) gene, which was probably acquired from its host. The function of the NblA protein is to disassemble phycobilisomes, cyanobacterial light harvesting complexes that can comprise up to half of the cellular soluble protein content. NblA thus plays an essential dual role in cyanobacteria: it protects the cell from high light intensities and increases the intracellular nitrogen pool under nutrient limitation. NblA has previously been shown to interact with phycocyanin, one of the main components of phycobilisomes. Using structural modeling and protein-protein docking, we show that the NblA dimer of Ma-LMM01 is predicted to have a significantly higher binding affinity forM. aeruginosaNIES-298 phycocyanin (αβ)6hexamers, compared to the host homolog. Protein-protein docking suggests that the viral NblA structural model is able to bind deeper into the phycocyanin groove. The main structural difference between the virus and host NblA appears to be an additional α-helix near the N-terminus of the viral NblA, which could be partly responsible for the deeper binding into phycocyanin. This unique helical region, absent in the cellular NblA, would be expected to constitute a viral evolutionary innovation. We propose that a higher binding affinity of NblA to the host phycocyanin may represent a selective advantage for the virus, whose rapid infection cycle requires an increased phycobilisome degradation rate that is not fulfilled by the NblA of the host.
Risk and outcomes of healthcare-associated infections in three hospitals in Bobo Dioulasso, 2022 (Burkina Faso): a longitudinal study
Authors: Hema Arsène , Some Satouro Arsène , Kaboré Odilon , Sanou Soufiane , Poda Armel , Meda Ziemlé Clément , Ouedraogo Abdoul Salam , Savadogo Léon. 
Published: July 2024

AbstractBackgroundHealthcare-associated infections (HAIs) are one of the most common adverse events in healthcare and represent a major public health problem. However, 40% to 70% of HAIs are considered to be preventable. The present study was conducted to analyze the incidence, etiological factors, and outcomes of HAIs through active surveillance in three hospitals in the city of Bobo Dioulasso.MethodsA prospective, longitudinal, multicenter study was conducted from May 1thto November 30rd, 2022, in two district hospitals (DO and Dafra) and the Sourô Sanou Teaching Hospital (CHUSS). Consenting patients hospitalized for reasons other than infection, cancer, immunosuppression in the postoperative care ward of DO or of Dafra district hospitals, intensive care unit (ICU)/CHUSS, neonatal ward/CHUSS, and gynecology and obstetrics postoperative care ward/CHUSS during a 2-month inclusion period in district hospitals and 4 months for CHUSS wards. For this study, we used the operational definitions of the French Technical Committee for Nosocomial Infections and Healthcare-associated Infections, with slight modifications. Logistic regression was used to analyze predictors of HAIs.ResultsOf the 664 patients enrolled, 166 experienced an HAI, with a cumulative incidence rate of 25% (CI: 21.7%-28.3%) or an incidence density rate of 36.7 per 1000 patient-days (CI: 31.7-42.9). Surgical site infections (SSI) (44%), followed by neonatal infections (42%) were the most common HAIs. Enterobacteriaceae represented 60% of the bacteria identified in HAIs, and 38.9% of them were extended spectrum β-lactamase (EBLSE) producers. Factors associated with HAIs were admission in the neonatal ward (aOR=7.4; CI:1.3-42.7), ICU (aOR=3.7; CI:1.4-9.5), hospital stay longer than 2 days (aOR=2.1; CI:1.2-3.4), or male sex (aOR=1.8; CI:1.1-3.1). In addition, HAIs were associated with longer follow-up, hospitalization, and mortality (18.1%; 95% CI:12.1 - 24.4). Deaths were only recorded in the ICU and neonatal ward, with case fatality rates of 45.4% (95% CI: 27.5 - 63.4) and 21.4% (95% CI: 11.6 - 31.3), respectively, p=0.019.ConclusionsThe incidence of HAIs was relatively high in the three hospitals in Bobo Dioulasso. A national strategy to reduce HAIs should be implemented to achieve better control of HAIs.
G-quadruplex formation in long non-coding RNAs dysregulated in colorectal cancer
Authors: Sharma Shubham , Shukla Chinmayee , Mitteaux Jérémie , Pipier Angélique , Pirrotta Marc , Penouilh Marie-José , Monchaud David , Datta Bhaskar 
Published: July 2024

AbstractNon-coding RNAs (ncRNAs) in human cells do not lead to protein synthesis and constitute a substantial portion of the transcriptome. Human long non-coding RNAs (lncRNAs) orchestrate critical cellular functions influencing development, differentiation, and metabolism. Dysregulation of lncRNAs has been correlated with several pathological conditions such as neurodegenerative and autoimmune disorders, diabetes, and cancer. Recent reports have suggested the involvement of G4s in lncRNAs to regulate colorectal cancer (CRC) carcinogenesis. In this study, we investigate the occurrence and distribution of G4s in theLINC01589,MELTF-AS1,andUXT-AS1lncRNAs, which have been reported to be dysregulated in CRC. Using a combination ofin silicotools andin vitrobiophysical techniques, we show that these lncRNAs form stable, parallel, and intramolecular G4s. Furthermore, we establish the formation of G4s within these lncRNAs in CRC using cell-based assays, including RNA G4-Immuno-FISH and G4RP-RT-qPCR. This is the first systematic study of G4s in lncRNAs dysregulated in CRC, and our findings highlight the diagnostic and therapeutic potential of G4s in CRC.
Examining the issues related to consumer co-created value: from policy perspective
Authors: Agrawal Amit Kumar , Rahman Zillur , Hussain Zahid , Chatterjee Sheshadri 
Published: July 2024

Purpose
This research article investigates the legal issues related to consumer co-created value (CCV) especially from the business law perspective. This study also aims to investigate how the active participation of consumers, firms and fellow consumers influences consumer CCV from the policy perspectives.

Design/methodology/approach
A research model has been developed to explore the connections between various constructs through an extensive examination of prior literature. There are ten hypotheses which were framed and were tested using structural equation modelling technique.

Findings
The results indicated that CCV mediates the relationship between customer satisfaction and antecedents of CCV. This has a ramification from the business law perspective. It was found that enhanced, benefit laden, experientially rich and interactive value is the key driver behind consumer participation and improving satisfaction level of the participating consumers and has policy implications.

Research limitations/implications
This study examines the co-creation related issues from the business law standpoint. This study suggests that value co-creation can be used as a strategy to achieve competitive advantage by inviting active consumer participation which can be key to achieve satisfaction of consumers. Legal fraternity and policymakers can use the findings to improve value creation and delivery to their consumers.

Originality/value
This study holds value both for legal fraternity, researchers and business law practitioners as emergence of internet has changed the way in which banking industry used to operate and has opened vistas for the organisations to allow customers to be part of value creation and enhancing their experience and satisfaction multifield time. Therefore, management researchers and policymakers would be intrigued with processes and tools through which consumers and fellow consumers can mutually benefit and also exchange value with each other with organisations acting as catalyst to the process from policy standpoint.
Student accommodation characteristics, perceived overall satisfaction and academic performance: evidence from six Scottish universities
Authors: Zaman Fahim Uz , Hosain Md Sajjad 
Published: July 2024

Purpose
The aim of this empirical study is to identify the relationship between student accommodation characteristics (SACs) and academic performance (AP). SACs, considered as the single independent variable, were divided into three further constructs: perceived location advantage (PLA), perceived facilities (PFs) and perceived safety and security (PSS). This study also intends to uncover the mediating role of perceived overall satisfaction (POS) over the direct relationships.

Design/methodology/approach
The study used a cross-sectional quantitative survey methodology to investigate the hypothesized relationships based on 384 sample responses (n) selected using purposive sampling techniques from six Scottish universities. The study used IBM SPSS version 29 and partial least squares-structural equation modeling version 4 for descriptive statistics and for testing the hypotheses, respectively.

Findings
Based on survey responses and the application of proper statistical measures, this study found that all three independent constructs (PLA, PFs and PSS) have a significant positive association with the dependent variable, AP. The mediator (POS) was also found to be positively correlated with AP. Furthermore, this study identified that POS can partially mediate all three direct relationships.

Originality/value
By extending and validating current theories such as satisfaction frameworks, student engagement models and servicescape psychology, this study can significantly advance the current state of the literature. It highlights how crucial holistic satisfaction can mediate the association between academic achievement and the features of student housing. The results provide theoretical justification for incorporating accommodations into planning and policy for education. Additionally, by emphasizing on the crucial elements that enhance students’ well-being and academic achievement, such as location, safety, aesthetics and extensive support services, the study offers significant management insights.
Properties and characterization of cross-linked VAc-VeoVa10 latex emulsified with reactive surfactants
Authors: Chen Lijun , Zhao Wanting , Gong Zheqing 
Published: July 2024

Purpose
The traditional vinyl ester of neodecanoic acid-vinyl acetate (VeoVa10-VAc) copolymer latex is a linear structure with poor film formation, thus causing solvent resistance and wear resistance of the latex film to be poor. This study aims to investigate the use of cross-linkers in emulsion polymerization to modify the latex. During the course of film formation, the reactive functional groups react to form cross-linkage. The network structure can effectively improve the compactness of the resin, thereby greatly improving the water resistance, solvent resistance and heat resistance of the resultant film. In addition, the reactive emulsifier is used to replace the conventional emulsifier. Thus, the drawbacks of the conventional emulsifier molecules migrate and desorb can be avoided when the polymer latex is stored.

Design/methodology/approach
The cross-linked VAc-VeoVa10 latex has been synthesized with the reactive surfactants, in which VAc and VeoVa10 are used as the main monomers and ethylene glycol dimethacrylate (EGDMA) was used as the cross-linked monomer. Potassium persulfate (KPS) and mixed surfactants of alkyl allyl polyoxyethylene ether ammonium sulfate (JS-20) and allyl nonyl phenol polyoxyethylene ether (ANPEO-10) were used as the initiator and emulsifier, respectively. The structure of resultant latex film was characterized by Fourier transform infrared spectroscopy. The latex films were tested by thermogravimetric analysis, differential scanning calorimetry and contact angle (CA). The particle size and its distribution of the latex were measured by the nano particle size analyzer.

Findings
The factors that had an influence on the properties of the latex and the film were investigated in detail. The stability of the resultant latex is good. The average particles of the latex and its distribution are small and uniform, respectively. In comparison with the conventional latex film, the thermal stability and hydrophobicity of the resultant latex film are improved obviously.

Practical implications
The resultant latex can be used in both the waterborne interior and exterior wall coatings, pickering stabilized waterborne polymer dispersions, polymer powders, environmentally friendly polymer-modified waterproof mortar and other fields, which can be satisfied with the high demand of thermal stability and hydrophobicity.

Originality/value
The modification of poly (VAc-VeoVa10) by reactive emulsifier and cross-linker is seldom reported. In this study, the cross-linked poly (VAC-VeoVa) latex is prepared through the reactive surfactants, with VAc and VeoVa10 used as the main monomers and EGDMA used as the cross-linked monomer. KPS and mixed surfactants of JS-20 and ANPEO-10 are used as the initiator and emulsifier, respectively.
Exploring the bidirectional causal associations between pain and circulating inflammatory proteins: A Mendelian randomization study
Authors: Wang Yu , Zhou Wenyu , Zhang Faqiang , Wei Juan , Wang Sheng , Min Keting , Chen Yuanli , Yang Hao , Lv Xin 
Published: July 2024

AbstractMultisite chronic pain (MCP) and site‐specific chronic pain (SSCP) may be influenced by circulating inflammatory proteins, but the causal relationship remains unknown. To overcome this limitation, two‐sample bidirectional Mendelian randomization (MR) analysis was used to analyse data for 91 circulating inflammatory proteins, MCP and SSCP encompassing headache, back pain, shoulder pain, hip pain, knee pain, stomach abdominal pain and facial pain. The primary MR method used was inverse variance weighting, sensitivity analyses included weighted median, MR pleiotropy residual sum and outlier and the Egger intercept method. Heterogeneity was also detected using Cochrane's Q test and leave‐one‐out analyses. Finally, a causal relationship between 29 circulating inflammatory proteins and chronic pain was identified. Among these proteins, 14 exhibited a protective effect, including MCP (T‐cell surface glycoprotein cluster of differentiation 5), headache (4E‐binding protein 1 [4EBP1], cluster of differentiation 40, cluster of differentiation 6 and C‐X‐C motif chemokine [CXCL] 11), back pain (leukaemia inhibitory factor), shoulder pain (fibroblast growth factor [FGF]‐5 and interleukin [IL]‐18R1), stomach abdominal pain (tumour necrosis factor [TNF]‐α), hip pain (CXCL1, IL‐20 and signalling lymphocytic activation molecule 1) and knee pain (IL‐7 and TNF‐β). Additionally, 15 proteins were identified as risk factors for MCP and SSCP: MCP (colony‐stimulating factor 1, human glial cell line‐derived neurotrophic factor and IL‐17C), headache (fms‐related tyrosine kinase 3 ligand, IL‐20 receptor subunit α [IL‐20RA], neurotrophin‐3 and tumour necrosis factor receptor superfamily member 9), facial pain (CXCL1), back pain (TNF), shoulder pain (IL‐17C and matrix metalloproteinase‐10), stomach abdominal pain (IL‐20RA), hip pain (C‐C motif chemokine 11/eotaxin‐1 and tumour necrosis factor ligand superfamily member 12) and knee pain (4EBP1). Importantly, in the opposite direction, MCP and SSCP did not exhibit a significant causal impact on circulating inflammatory proteins. Our study identified potential causal influences of various circulating inflammatory proteins on MCP and SSCP and provided promising treatments for the clinical management of MCP and SSCP.
Faithful men and false women: Love‐suicide in early modern English popular print
Authors: Knox Imogen 
Published: July 2024

AbstractThis article explores the representation of suicide committed for love in English popular print in the seventeenth and early eighteenth centuries. It shows how, within ballads and pamphlets, suicide resulting from failed courtship was often portrayed as romantic and an expression of devotion. Within this literature, however, young women often bore the blame for male suicide, influenced by early modern ideas about the innate characteristics of men and women. This examination interrogates a context in which suicide, rather than being condemned, was consistently depicted sympathetically. It also sheds crucial light on the representation and performance of masculinity and femininity in courtship and matters of love.