bluuebunny/crossref_metadata_2025_split huggingface dataset:
A MUSICALIZAÇÃO E APRENDIZAGEM: AS CONTRIBUIÇÕES DA MÚSICA NO CONTEXTO ESCOLAR
Authors: Dos Reis Silva Julia Nathaly , Alves dos Reis Elisangela , Pereira De Souza Ferrari Meire , Gaiotto Paulo Alexandre 
Published: February 2025

O objetivo desta pesquisa é apresentar os pressupostos teóricos que destacam a relevância da musicalização no espaço escolar, a fim de compreendê-la como um recurso potencialmente significativo que pode contribuir para o desenvolvimento das funções psicológicas superiores, sobretudo no que se refere à atenção, concentração e memória. Para tanto, levantamos as seguintes problemáticas: Qual o conceito de música? Qual a relevância da música no contexto escolar? Qual o histórico da educação musical no Brasil? Quais práticas de musicalização podem ser utilizadas pelos docentes? Partimos do pressuposto de que a musicalização promove um ambiente receptivo e acolhedor para a aprendizagem pois, representa uma importante fonte de estímulos, equilíbrio e felicidade para os estudantes. Trata-se de uma pesquisa cujo caráter é bibliográfico, toma por base os aportes teóricos que discutem a temática e demonstra a importância da musicalização no contexto escolar; a linguagem musical como recurso lúdico de aprendizagem; a música no contexto do componente curricular de Arte; legislação e a educação musical; o valor educativo da música e metodologias para trabalhar a educação musical. Nesse sentido, apresentamos o conceito de música, a história e a memória da educação musical no Brasil, a Arte enquanto disciplina e componente curricular na escola, com intuito de problematizar sobre a relevância da música e seus benefícios no melhoramento das capacidades cognitiva, social e cultural, colaborando em diversas áreas do conhecimento como a matemática, linguagens e filosofia. Em virtude dessa flexibilidade, a interdisciplinaridade entre a música e a educação é vista como um poderoso instrumento para viabilizar um crescimento exponencial da capacidade de aprendizagem de inúmeros discentes, possibilitando um ambiente de inclusão e socialização.
ANÁLISE COMPARATIVA DE MÉTODOS DE INTERPOLAÇÃO ESPACIAL APLICADOS A DADOS DE RESERVATÓRIO DE PETRÓLEO
Authors: Pelegrino Lohann , Aciole Diego , De Souza Grazione , Custódio Lis 
Published: February 2025

Na exploração e produção de óleo e gás, o alto custo da aquisição das informações do sistema poço-reservatório, como as propriedades da formação rochosa e as características de sua geometria, fazem com que a coleta de dados utilizados para estimar o comportamento de um dado reservatório seja realizada em um número limitado de localizações. Uma vez obtido os dados, os métodos de interpolação desempenham um papel crucial no mapeamento da propriedade estudada no reservatório sob análise, permitindo que seja possível estimar seus valores em regiões de interesse em função dos dados previamente coletados. Tendo em vista este cenário, é importante conhecer as limitações do método de interpolação adotado, assim como seu desempenho quando aplicado a diferentes configurações de dados. Neste trabalho, apresentamos uma análise comparativa de diferentes métodos de interpolação espacial aplicados na estimativa das propriedades de rochas de um reservatório de petróleo.
Modelagem do Perfil de 6.2 μm
Authors: Avelaneda Vitor das Neves , Canelo Carla Martinez , Sales Dinalva Aires de , Fischer Julia de Campos 
Published: February 2025

Estudar os hidrocarbonetos aromáticos policíclicos (PAHs) é fundamental para o entendimento do meio interestelar (ISM) de galáxias, especialmente por estarem presentes em quase todos ambientes astrofísico, desde regiões de formação estelar até núcleos ativos de galáxias (AGNs). Uma característica peculiar dessas moléculas pode ser encontrada nas variações do perfil da banda de 6.2 μm, essa banda pode indicar o processo de nitrogenação dos anéis aromáticos dos PAHs. Neste trabalho, 279 galáxias com emissão dominada por AGN (AGN-dominated), extraídas do projeto Spitzer/IRS ATLAS, tiveram seu contínuo e perfil de 6.2 μm modelados e classificados conforme as classes de Peeters (A, B e C). Os resultados obtidos neste estudo mostram uma predominância de objetos classe A que só conseguem ser explicados pela presença de Heterocíclicos Policíclicos Aromáticos Nitrogenados (PANHs).
In silico and In vitro Analysis of Coniferin Mediates Apoptosis by Intervening the
PTGS2/Apoptosis Pathway to Alleviate Rheumatoid Arthritis
Authors: Shi Mei-Feng , Ma Xiao-Na , Zou Fang-shu , Feng Wei , Xu Qiang 
Published: February 2025

Purpose:
Liquidambaris Fructus (LF), a prevalent Chinese medicinal herb, has been effectively utilized
in the clinical treatment of rheumatoid arthritis (RA). Coniferin, is the active ingredient in LF, and there
is a paucity of research examining its potential anti-RA properties. This study employs in silico analysis and
experimental validation to delve into the therapeutic potential of Coniferin against RA and to elucidate its
mechanism of action.

Methods:
In silico Analysis was employed to construct a drug-disease target protein-protein interaction (PPI)
network, to perform Functional enrichment analysis, and to molecular docking of the principal compounds
and target proteins. Subsequently, the effects of coniferin on the proliferation, migration, and invasion of
rheumatoid arthritis - Fibroblast-like synoviocytes (RA-FLSs) were observed using a CCK8 assay and
Transwell assay. ELISA was employed to detect the inflammatory response of RA-FLSs in coniferin. Flow
cytometry was utilized to detect the effects of coniferin on apoptosis, oxidative stress, and mitochondrial
transmembrane potential in RA-FLSs. Ultimately, the expression of pivotal proteins and apoptosis markers
within the PTGS2/Apoptosis signaling pathway was discerned through the utilization of Real-time quantitative
PCR (RT-qPCR) and Western blot.

Results:
It was observed that coniferin promotes apoptosis of RA-FLSs through the PTGS2/Apoptosis signaling
pathway and inhibits the proliferation, migration, and invasion of RA-FLSs, with anti-inflammatory, oxidative
stress-reducing, and mitochondrial transmembrane potential disruption effects.

Conclusion:
The potential mechanism of coniferin for the treatment of RA is to promote apoptosis of RAFLSs
by intervening in the PTGS2/apoptosis signaling pathway.
Identification of Mitochondrial-related Characteristic Biomarkers in
Osteosarcoma using Bioinformatics and Machine Learning
Authors: Hou Jingyi , Zhang Yu , Yang Ning , Chen Bin , Chang Chengbing , Gu Haipeng , Liu Yanqi , Zhu Naiqiang 
Published: February 2025

Background/Aims:
Osteosarcoma (OS), a malignant tumor originating in bone or cartilage,
primarily affects children and adolescents. Notably, substantial alterations in mitochondrial
energy metabolism have been observed in OS; however, the specific contribution of mitochondrial-
related genes (MRGs) to OS pathogenesis and prognosis remains unclear. Herein, we
identified novel diagnostic biomarkers associated with mitochondrial-related processes in OS via
comprehensive bioinformatics analysis.

Methods:
OS mRNA expression profiles were retrieved from GSE16088 and GSE19276 databases.
Mitochondrial-related differentially expressed genes (MitoDEGs) were identified by integrating
differentially expressed analysis with mitochondrial-localized genes. A protein-protein
interaction network was constructed, and machine learning algorithms (LASSO regression analysis
and SVM-RFE) identified characteristic MitoDEGs. Subsequently, immune cell infiltration,
microenvironment analysis, and single-cell RNA sequencing (scRNA-seq) analyzed differences
in characteristic MitoDEGs, and RT-PCR was used for in vitro verification of characteristic MitoDEGs.

Results:
MitoDEGs in OS were significantly enriched in the pathways associated with mitochondrial
function and immune regulation. Two MitoDEGs, UCP2 and PRDX4, were identified
via LASSO and SVM-RFE. Correlation analysis demonstrated a close association between
UCP2 and PRDX4 expression levels and immune cell infiltration, particularly in CD8+ T and native
CD4+ T cells, as observed in both immune cell and scRNA-seq analyses. Furthermore, RTPCR
confirmed the expression levels of UCP and PRDX4 at the cellular level, which was consistent
with the bioinformatics results.

Conclusion:
This study identified UCP2 and PRDX4 as characteristic MitoDEGs and potential
diagnostic biomarkers for OS using machine learning algorithms. These findings provide novel
insights into the clinical applications of these biomarkers for OS diagnosis.
2-DG Promotes the Proliferation, Differentiation, Migration, and Resistance to Oxidative Stress of Mesenchymal Stem Cells through Hippo Signaling
Authors: Dong Liang , Li Lang 
Published: February 2025

Background:
Hippo signaling regulates the behavior and fate of
mesenchymal stem cells (MSCs), which are crucial for the repair and cure of acute
respiratory distress syndrome (ARDS). However, whether 2-deoxy-D-glucose (2-DG),
a specific activator of Hippo signaling, would further enhance the reparative effect of
MSCs in ARDS remains unclarified.

Objective:
This study aimed to determine whether 2-DG could promote the
proliferation, differentiation, migration, and resistance to oxidative stress of mouse
bone marrow-derived MSCs (mBMSCs).

Methods:
mBMSCs were isolated from C57BL/6 mice and differentiated into alveolar
type II epithelial (ATII) cells by noncontact coculture. The specific activator and
inhibitor 2-DG and 4-[(5,10-dimethyl-6-Oxo-6,10-dihydro-5h-pyrimido[5,4-B]thieno[3,2-
E][1,4]diazepin-2-Yl)amino]benzenesulfonamide (XMU-MP-1) were used to activate
and inhibit Hippo signaling, respectively. Oxidative stress-induced injuries were
induced by H2O2 treatment.

Results:
We observed that 2-DG activated Hippo signaling and promoted mBMSC
proliferation in a dose-dependent manner. 2-DG also promoted the differentiation of
mBMSCs into ATII cells and enhanced not only the horizontal and vertical migration of
mBMSCs but also mBMSC homing to injured lung tissue. H2O2 treatment inhibited
Hippo signaling and reduced the viability of mBMSCs by decreasing the Bcl-2/Bax
ratio, but 2-DG activated Hippo signaling and conferred mBMSCs with resistance to
oxidative stress by increasing the Bcl-2/Bax ratio. However, XMU-MP-1 suppressed
these effects to some extent.

Conclusion:
Through Hippo signaling, 2-DG promoted the proliferation, migration,
differentiation, and resistance to oxidative stress of mBMSCs, suggesting a novel
strategy for enhancing the reparative effects of MSCs in ARDS.
Anti-Alzheimer’s, Anticancer, and Antimicrobial Activities of Transition Metal Complexes of 4-[(E)-(5-chloro-2-hydroxy-3-{(E)-(3-nitrophenyl)
diazenyl}benzylidene)amino]-1,5-dimethyl-2-phenyl-1H-pyrazol-3(2H)-one
Authors: Sarwade Kirti Navnath , Thakur Shailendrasingh Virendrasingh , Sakhare Kuldeep Bhaskar , Sakhare Mahadeo Abarao , Bharate Yogesh Navnath 
Published: February 2025

Background:
Age-related Alzheimer's disease is a common neurodegenerative
disease. The number of Alzheimer's patients worldwide is expected to reach approximately 150
million by 2050. Breast cancer is one of the most common cancers in females, and its prevalence
rises each year. Annually, worldwide, almost 500,000 women pass away due to breast cancer.
Current treatments for Alzheimer’s disease and cancer aim to stop or slow the progression of the
disease. Developing more effective methods of treatment has become a global effort. Many
scientists from around the world are busy in finding effective treatment solutions for Alzheimer’s
disease and cancer. The metal-ligand chemistry shows a variety of applications, including
physiological and pharmacological activities.

Methods:
The green and efficient synthesis of coordination complexes of novel azo-Schiff
base ligand 4-[(E)-(5-chloro-2-hydroxy-3-{(E)-(3-nitrophenyl)diazenyl}benzylidene)amino]-1,5-
dimethyl-2-phenyl-1H-pyrazol-3(2H)-one was performed by grinding technique. Further, metal
salts were introduced to this azo-Schiff base ligand, and metal complexes were synthesized. These
synthesized products were characterized by 1H-NMR spectra, mass spectra, FTIR spectra, electronic
spectra, elemental analysis, thermal analysis, X-ray powder diffraction, molar conductivity,
etc., and their anti-Alzheimer’s, anticancer, and antimicrobial activities were assessed by MTT
assay and disc diffusion method, respectively.

Result:
The structures of the azo-Schiff base ligand and its metal complexes were confirmed by
using various spectroscopic techniques. In the biological activity study, many complexes showed
higher inhibition than the standard reference drug.

Conclusion:
The synthesized metal complexes exhibited higher biological activities than the azo-
Schiff base ligand. All metal complexes, except for some, served as anti-Alzheimer’s and anticancer
agents.
Melittin, A Potential Game-changer in the Fight Against Breast Cancer: A Systematic Review
Authors: Prince Gilles , Assi Ahmad , Aoude Marc , Kourie Hampig Raphael , Haddad Fadi 
Published: February 2025

Introduction:
Breast cancer is the most common cancer in women. Traditional treatments include
endocrine therapy, chemotherapy, surgery, radiation, and immunotherapy. Recent studies suggest melittin, a
component of bee venom, as a promising breast cancer treatment due to its anticancer properties: inducing cytotoxicity,
apoptosis, and gene regulation.

Method:
This manuscript aims to review melittin's potential therapeutical and future implications in treating
breast cancer. An extensive literature search was conducted on MEDLINE and Cochrane databases up to July
2024 using Boolean operators with a combination of keywords. After screening, data extraction, and descriptive
analysis, 40 articles were retained.

Results:
Experimental data and different therapeutical strategies were collected. Melittin disrupts tumor cell
membranes and modulates key apoptotic pathways. Advanced delivery systems enhance their effectiveness and
reduce toxicity. Combining melittin with chemotherapy shows synergistic effects, improving outcomes. Thus,
melittin could be a valuable addition to breast cancer therapies.

Conclusion:
Further clinical trials are essential to validate its potential and establish its role in breast cancer therapy.
Preliminary Investigations on Acyl Hydrazones Bearing Sulfonamides as Inhibitors of the Human Carbonic Anhydrase Isoforms I, II, IX, and XII
Authors: Dincel Efe Doğukan , Kuran Ebru Didem , Ece Abdulilah , Başoğlu-Ünal Faika , Renzi Gioele , Badii Gloria , Carta Fabrizio , Supuran Cladiu T. , Ulusoy-Güzeldemirci Nuray 
Published: February 2025

Aim:
The present study aims to identify the synthesis and structural characterization of acyl hydrazone-
sulfonamide-containing compounds that were tested in vitro on human carbonic anhydrase (hCA) isoforms
I, II, IX, and XII.

Methods:
Herein, acyl hydrazone derivatives containing the primary sulfonamide moiety were synthesized via a
three-step synthetic pathway starting from the commercially available 4-sulfamoyl benzoic acid. Structural characterizations
of the final compounds were assessed through IR IR, 1H-NMR, 13C-NMR, and elemental analyses.
The in vitro profiling activity of the final compounds on the Carbonic Anhydrases (CAs; EC 4.2.1.1) I, II, IX, and
XII were performed by means of the stopped-flow technique and revealed nanomolar inhibitory potencies on the
selected targets. Molecular docking and molecular dynamic simulations afforded a detailed understanding of the
binding modes of the most effective compounds.

Results:
We reported the synthesis and structural characterization of 25 acyl hydrazone-sulfonamide-containing
compounds that were tested in vitro on the hCAs I, II, IX, and XII isoforms for their inhibitory features. Overall,
all compounds showed nanomolar inhibition potencies on the panel of hCAs considered, and their binding modes
were deciphered by means of in-silico studies. Molecular docking followed by MD simulations confirmed the
stability of 4l-hCA I, 4n-hCA II, 4t-hCA II, 4v-hCA XII, and 4w-hCA XII complexes.

Conclusion:
This study presents a deep understanding of the structural determinants influencing the affinity and
selectivity of the designed compounds towards different hCAs, thus offering valuable insights for further optimization
and development in the field.
Sphingolipids in Cancer: Metabolism, Signaling, and Clinical Implications
Authors: Desai Sharav A. , Nagare Sandip D. , Patel Vipul P. , Pagare Nishant B. , Jangam Tejas C. 
Published: February 2025

Abstract:
Sphingolipids are bioactive lipids that are essential for cellular functions like signaling, apoptosis, and proliferation. They are also important in the biology of cancer. The complex dynamics of sphingolipid metabolism and its consequences for the advancement of cancer are examined in this review. It highlights the regulatory functions of important enzymes such as ceramide kinase (CERK) and sphingosine ki-nases (SPHKs) in preserving the equilibrium between sphingosine-1-phosphate (S1P), a pro-survival chemical, and ceramides, which encourage cell death. Tumour growth, metastasis, and treatment resistance are all significantly affected by disturbances in this equilibrium. The review emphasizes the potential of sphingolipids as biomarkers for cancer prognosis and stratification, providing information on the course of the dis-ease and the effectiveness of treatment. Their crucial functions in cellular signalling pathways that affect angiogenesis, immunological evasion, and drug resistance, all of which are linked to cancer, are also reviewed. Their role in the tumor microenviron-ment further highlights sphingolipids' significance as targets for novel therapeutic ap-proaches. Improved clinical results and personalized cancer treatments are made pos-sible by developments in sphingolipid biology and their potential as biomarkers. This thorough synthesis provides the groundwork for further studies that will use sphin-golipid metabolism and signalling to create potent cancer treatments. In the fight against cancer, we can improve therapeutic efficacy and diagnostic accuracy by under-standing these intricate relationships.
Pharmacognostic, GC-MS, and In Silico Molecular Docking Studies of Phytoconstituents from Urtica dioica and Zanthoxylum armatum for Male Antifertility Potential
Authors: Wadhawan Swati , Chaudhary Anurag , Koul Sameksha 
Published: February 2025

Background:
Research on non-hormonal alternatives to traditional methods of male contraception has been prompted by the growing world population as well as the need for effective and reversible treatments. This study aimed to examine the possible antifertility potential of two medicinal herbs, i.e., Urtica dioica and Zanthoxylum armatum, that have been utilized for centuries in different cultures.

Methodology:
A thorough pharmacognostical analysis was carried out to identify the ingre-dients of these plants. The phytoconstituents from Urtica dioica aqueous 96% methanolic leaf extract and Zanthoxylum armatum fruit essential oil were subjected to in silico molec-ular docking screening. The phytoconstituents were profiled using GC-MS analysis. To in-vestigate how these phytoconstituents interact with important proteins involved in male fer-tility and identify potential candidates for the development of a plant-based oral contracep-tive for men, further in silico molecular docking (receptor protein ID of 7FIH (3.20 Å), 7BPR (1.95 Å), and 2Q7J (1.90 Å) ) experiments were carried out.

Results:
The GC-MS analysis revealed a wide variety of bioactive chemicals. Promising interactions between the discovered phytoconstituents and specific protein targets were found by the in silico molecular docking investigation, indicating the possibility of antifertil-ity effects.

Conclusion:
The results of this study demonstrate the medicinal use of these plants and provide the basis for the development of novel male contraceptive medicines and their fur-ther experimental validation.
The Use of Technology in Enhancing Nurses' Pain Management Competencies: A Narrative Review
Authors: Afolalu Olamide O. , Afolalu Adeniran S. , Akpor Oluwaseyi A. 
Published: February 2025

Background:
Effective pain management is a critical aspect of nursing care, and technological advancements have the potential to improve nurses' competency in assessing, monitoring, and intervening as a strategy for improved patients' pain experiences and outcomes.

Objective:
This review aimed to explore various technologies employed in pain management, their implications on nurses' competencies, and the challenges and benefits associated with their implementation.

Methods:
Using keywords from relevant studies, we searched the following electronic databases for pertinent literature and freely accessible full text: PubMed, ScienceDirect, IEEE Xplore, and Google Scholar.

Results:
Findings from the literature provide valuable insights into the various technologies employed by nurses to assess pain, such as wearable technology, virtual reality, mobile applications, and telehealth platforms, that give nurses a chance to develop their expertise in pain management, put evidence-based interventions into practice, and track patient response to care. Additionally, the benefits of implementing technology applications in pain management, including its ability to broaden nurses’ knowledge, hone their decision-making skills, and customize patient care with the use of simulation platforms and remote monitoring tools, were identified. Furthermore, issues like technological literacy, time restraints, privacy concerns, and ethical considerations need to be addressed for the effective incorporation of technology into pain management procedures.

Conclusion:
To improve patient care and outcomes, nurses can use technology to improve their pain management skills by recognizing the possible benefits and resolving related problems. Conclusively, areas for future research and development and implications to nursing practice, education, and research were outlined.
Effectiveness of AI for Enhancing Computed Tomography Image Quality and Radiation Protection in Radiology: Systematic Review and Meta-Analysis
Authors: Zhang Subo , Zhu Zhitao , Yu Zhenfei , Sun Haifeng , Sun Yi , Huang Hai , Xu Lei , Wan Jinxin 
Published: February 2025

Background
Artificial intelligence (AI) presents a promising approach to balancing high image quality with reduced radiation exposure in computed tomography (CT) imaging.

Objective
This meta-analysis evaluates the effectiveness of AI in enhancing CT image quality and lowering radiation doses.

Methods
A thorough literature search was performed across several databases, including PubMed, Embase, Web of Science, Science Direct, and Cochrane Library, with the final update in 2024. We included studies that compared AI-based interventions to conventional CT techniques. The quality of these studies was assessed using the Newcastle-Ottawa Scale. Random effect models were used to pool results, and heterogeneity was measured using the I² statistic. Primary outcomes included image quality, CT dose index, and diagnostic accuracy.

Results
This meta-analysis incorporated 5 clinical validation studies published between 2022 and 2024, totaling 929 participants. Results indicated that AI-based interventions significantly improved image quality (mean difference 0.70, 95% CI 0.43-0.96; P<.001) and showed a positive trend in reducing the CT dose index, though not statistically significant (mean difference 0.47, 95% CI –0.21 to 1.15; P=.18). AI also enhanced image analysis efficiency (odds ratio 1.57, 95% CI 1.08-2.27; P=.02) and demonstrated high accuracy and sensitivity in detecting intracranial aneurysms, with low-dose CT using AI reconstruction showing noninferiority for liver lesion detection.

Conclusions
The findings suggest that AI-based interventions can significantly enhance CT imaging practices by improving image quality and potentially reducing radiation doses, which may lead to better diagnostic accuracy and patient safety. However, these results should be interpreted with caution due to the limited number of studies and the variability in AI algorithms. Further research is needed to clarify AI’s impact on radiation reduction and to establish clinical standards.