bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen‐lactoferrin fibrillar coatings enhance osteoblast proliferation and differentiation
Authors: Vandrovcova Marta , Douglas Timothy E. L. , Heinemann Sascha , Scharnweber Dieter , Dubruel Peter , Bacakova Lucie 
Published: April 2014

AbstractLactoferrin is a milk‐derived glycoprotein with anabolic effects on the bone tissue. In this study, artificial extracellular matrices (aECM) consisting of collagen type I fibrils formed in the presence of lactoferrin at two different concentrations (0.5 and 1 mg mL−1) were prepared on the surface of poly(lactic‐co‐glycolic acid) (PLGA) foils. The aim of the study was to investigate the effects of aECM on the adhesion, growth and osteogenic differentiation of human osteoblast‐like Saos‐2 cells. On days 1 and 3 after seeding, higher numbers of cells were found on samples with collagen and collagen‐lactoferrin coatings (particularly on those formed at the higher concentration of lacroferrin) than on control microscopic glass coverslips. Cells on coatings formed in the presence of lactoferrin had more numerous and better developed vinculin‐containing focal adhesion plaques. On day 7, cells on coatings with and without lactoferrin produced significantly higher levels of osteocalcin than cells on control polystyrene cell culture dishes, the highest average values being found on samples with the lower concentration of lactoferrin. Expression of collagen I and alkaline phosphatase was on a similar level in cells on all tested samples and control polystyrene. Thus, lactoferrin promotes adhesion, growth and osteogenic differentiation of Saos‐2 cells and is promising as a bone implant coating component. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 525–533, 2015.
The role of fore‐ and hindlimbs during jumping in the Dybowski's frog (Rana dybowskii)
Authors: Wang Zhongyuan , Ji Aihong , Endlein Thomas , Samuel Diana , Yao Ning , Wang Zhouyi , Dai Zhendong 
Published: April 2014

AbstractAnurans are well known for their jumping abilities, making use of their strong hindlimbs. In contrast, the function of the forelimbs during take‐off has rarely been studied. We measured the ground reaction forces exerted by forelimbs and hindlimbs during short jumps in the Dybowski's frog Rana dybowskii. Take‐off occurred in two phases. Phase one (from the initial time until the forelimbs took off), which lasts a relatively long time (63.2 ± 4.1% of the total take‐off phase, N = 20), provides sufficient time for the forelimbs to elevate the body to a suitable posture to deliver the best take‐off angle. Phase two (from the forelimbs lift‐off until hindlimbs lift‐off) was dominated by the hindlimbs which provided a constant and fast elevation. The force angle (angle of the resultant vector from fore‐aft and normal force components towards the plane of the substrate) of the hindlimbs and body trajectory was variable before the forelimbs lifted off of the substrate and then primarily followed the direction of the line from the foot‐substrate point to the center of mass (COM). The preparation angle adopted when the forelimbs lifted off of the substrate was a good predictor of the take‐off angle. The total normal force oscillated around body weight (BW) before the forelimb normal force peaked. The BW shifted from the hindlimbs to the forelimbs during the initial phase of take‐off. A simple lever model suggests that the forelimbs are responsible for raising the COM, thus influencing the take‐off angle in short jumps. J. Exp. Zool. 321A: 324–333, 2014. © 2014 Wiley Periodicals, Inc.
Hedgehog pathway as a potential treatment target in human cholangiocarcinoma
Authors: Riedlinger Dorothee , Bahra Marcus , Boas‐Knoop Sabine , Lippert Steffen , Bradtmöller Maren , Guse Katrin , Seehofer Daniel , Bova Roberta , Sauer Igor M , Neuhaus Peter , Koch Arend , Kamphues Carsten 
Published: April 2014

AbstractBackgroundInnovative treatment concepts targeting essential signaling pathways may offer new chances for patients suffering from cholangiocarcinoma (CCC). For that, we performed a systematic molecular genetic analysis concerning the Hedgehog activity in human CCC samples and analyzed the effect of Hh inhibition on CCC cells in vitro and in vivo.MethodsActivation of the Hh pathway was analyzed in 50 human CCC samples using quantitative polymerase chain reaction (qPCR). The efficacy of Hh inhibition using cyclopamine and BMS‐833923 was evaluated in vitro. In addition, the effect of BMS‐833923, alone or in combination with gemcitabine, was analyzed in vivo in a murine subcutaneous xenograft model.ResultsExpression analysis revealed a significant activation of the Hh‐signaling pathway in nearly 50% of CCCs. Hh inhibition resulted in a significant decrease in cell proliferation of CCC cells. Moreover, a distinct inhibition of tumor growth could be seen as a result of a combined therapy with BMS‐833923 and gemcitabine in CCC xenografts.ConclusionThe results of our study suggest that the Hh pathway plays a relevant role at least in a subset of human CCC. Inhibition of this pathway may represent a possible treatment option for CCC patients in which the Hh pathway is activated.
Diagnostic yield of head computed tomography for the hospitalized medical patient with delirium
Authors: Theisen‐Toupal Jesse , Breu Anthony C. , Mattison Melissa L. P. , Arnaout Ramy 
Published: April 2014

BACKGROUNDDelirium is common in hospitalized patients and warrants early diagnosis and treatment. Often the evaluation of delirium includes head computed tomography imaging. However, in hospitalized medical patients, the yield of head computed tomography is unknown.OBJECTIVETo determine the diagnostic yield of head computed tomography when evaluating a hospitalized medical patient with delirium in the absence of a recent fall, head trauma, or new neurologic deficit.DESIGN AND SETTINGRetrospective medical record review at a large academic medical center in Boston, Massachusetts.PARTICIPANTSWe reviewed all medical records for head computed tomography scans performed from January 2010 through November 2012 in patients on the general medicine or medical subspecialties units.MAIN OUTCOMESA “positive” head computed tomography was defined as an intracranial process that could explain delirium. An “equivocal” head computed tomography was defined as the presence of a finding of unclear significance in relation to delirium.RESULTSThere were 398 patients hospitalized for >24 hours who underwent head computed tomography for delirium. Two hundred twenty head computed tomography studies met eligibility criteria, with 6 (2.7%) positive and 4 (1.8%) equivocal results. All positive and equivocal findings resulted in change in management.CONCLUSIONSThe diagnostic yield of head computed tomography in determining the cause of delirium in hospitalized patients is low. Due to the low rate of positive findings, head imaging is unnecessary in the majority of cases of delirium. However, there may be a subset of high‐risk individuals in which head imaging is indicated. Journal of Hospital Medicine 2014;9:497–501. © 2014 Society of Hospital Medicine
Simple enrichment of thiol‐containing biomolecules by using zinc(II)–cyclen‐functionalized magnetic beads
Authors: Fujioka Haruto , Tsunehiro Masaya , Kawaguchi Maho , Kuramoto Yasuhiro , Kurosaki Hiromasa , Hieda Yuhzo , Kinoshita‐Kikuta Emiko , Kinoshita Eiji , Koike Tohru 
Published: April 2014

A simple and efficient method based on magnetic‐bead technology has been developed for the enrichment of thiol‐containing biomolecules, such as l‐glutathione and cysteine‐containing peptides. The thiol‐binding site on the bead is a mononuclear complex of zinc(II) with 1,4,7,10‐tetraazacyclododecane (cyclen); this is linked to a hydrophilic cross‐linked agarose coating on a particle that has a magnetic core. All steps for the thiol‐affinity separation are conducted in aqueous buffers with 0.10 mL of the magnetic beads in a 1.5 mL microtube. The entire separation protocol for thiol‐containing compounds, from addition to elution, requires less than one hour per sample, provided the buffers and the zinc(II)–cyclen‐functionalized magnetic beads have been prepared in advance. The thiol‐affinity magnetic beads are reusable at least 15 times without a decrease in their thiol‐binding ability, and they are stable for six months at room temperature.
Clinical use of the Kessler psychological distress scales with culturally diverse groups
Authors: Stolk Yvonne , Kaplan Ida , Szwarc Josef 
Published: April 2014

AbstractThe Kessler 10 (K10) and embedded Kessler 6 (K6) was developed to screen for non‐specific psychological distress and serious mental illness in mental health surveys of English‐speaking populations, but has been adopted in Western and non‐Western countries as a screening and outcome measure in primary care and mental health settings. This review examines whether the original K6/K10's validity for culturally diverse populations was established, and whether the cultural equivalence, and sensitivity to change of translated or culturally adapted K6/K10s, has been demonstrated with culturally diverse client groups. Evidence for the original K6/K10's validity for culturally diverse populations is limited. Questions about the conceptual and linguistic equivalence of translated/adapted K6/K10s arise from reports of changes in item connotation and differential item functioning. Evidence for structural equivalence is inconsistent, as is support for criterion equivalence, with the majority of studies compromising on accuracy in case prediction. Research demonstrating sensitivity to change with culturally diverse groups is lacking. Inconsistent evidence for the K6/K10's cultural appropriateness in clinical settings, and a lack of clinical norms for either majority or culturally diverse groups, indicate the importance of further research into the psychological distress construct with culturally diverse clients, and the need for caution in interpreting K6/K10 scores. Copyright © 2014 John Wiley & Sons, Ltd.
Role of child weight status and the relative reinforcing value of food in children's response to portion size increases
Authors: Kral Tanja V.E. , Remiker Adriane M. , Strutz Erin M. , Moore Reneé H. 
Published: April 2014

ObjectiveFood portion size is an important determinant of intake in children. It remains unknown if children's weight status and relative reinforcing value of food (RRVF) interact with portion size to affect intake.MethodsIn a randomized crossover design, 25 normal‐weight and 25 obese children, ages 8‐10 years, consumed dinner once a week for 3 weeks. At each dinner, the same meal was served, but the portion size of all foods (chicken nuggets, hash browns, ketchup, green beans, brownies) and a sugar‐sweetened beverage (fruit punch) varied across conditions (100, 150, and 200%). Children's RRVF was assessed using a behavioral choice task.ResultsThere was a significant main effect of portion size condition (P = 0.003) and weight status (P = 0.0005) and a nonsignificant trend for a portion size‐by‐weight status interaction (P = 0.108) on intake. Mean intakes across conditions (100, 150, and 200%) were 801 ± 57, 964 ± 58, and 873 ± 57 kcal for normal‐weight children and 1041 ± 57, 1129 ± 57, and 1210 ± 57 kcal for obese children, respectively. Neither the main effect of RRVF status nor the condition‐by‐RRVF status interaction was significant (P > 0.48).ConclusionsEnvironments that offer large portions of palatable foods affect all children's intake irrespective of their weight status or how reinforcing they find food to be.
Constructing a performance‐based accountability system for the Chinese government
Authors: Tan XiuXia 
Published: April 2014

PurposeThis study constructs a valid performance‐based system to monitor accountability within the Chinese government.Design/methodology/approachFirst, a normative analysis model was constructed to include four factors, which form a complete accountability system: accountability to whom, accountability for what, how to demand accountability, and its effectiveness. This normative model was then used to explore existing practices of Chinese government performance evaluation and accountability.FindingsOur study revealed the need to accelerate ‘two integrations’ in order to develop performance‐based accountability. This would involve accelerating the integration of government performance assessment and accountability and accelerating the integration of external control and internal control.Research ImplicationsThis study is a preliminary study that points out the direction of performance‐based accountability system reform in Chinese government. It will further promote the transformation of government functions and change the way administration is carried out, promote to achieve the goal of the responsible government, and service‐oriented government. Establishing an effective performance‐based accountability system in China may yet require further research and policy recommendations.Originality/valueThis article is original in its attempt to construct an accountability index for the Chinese government. Its specific focus on China increases its originality as no known study has attempted to construct such an index. Copyright © 2014 John Wiley & Sons, Ltd.
Insulin pump therapy: a practical guide to optimising glycaemic control
Authors: Wilmot Emma G , Choudhary Pratik , Grant Paul , Hammond Peter 
Published: April 2014

AbstractInsulin pump services have been widely available in the UK for over 10 years. Despite this, the recent national Insulin Pump Audit identified that only 6% of patients with type 1 diabetes are managed with insulin pump therapy, far lower than anticipated. A key reason for the UK continuing to lag behind other European countries in the provision of insulin pump services is the lack of trained health care professionals.This paper aims to provide diabetologists and diabetes specialist nurses with a basic understanding of the clinical approach to the patient with type 1 diabetes on insulin pump therapy. Copyright © 2014 John Wiley & Sons.
Notoriety bias in a database of spontaneous reports: the example of osteonecrosis of the jaw under bisphosphonate therapy in the French national pharmacovigilance database
Authors: de Boissieu Paul , Kanagaratnam Lukshe , Abou Taam Malak , Roux Marie‐Paule , Dramé Moustapha , Trenque Thierry 
Published: April 2014

ABSTRACTPurposeThe purpose of this study was to identify a notoriety bias in a database of spontaneous reports and its consequences on the calculation of the reporting odds ratio (ROR).MethodsWe used the case/noncase methodology to calculate the ROR for bisphosphonates and osteonecrosis of the jaw (ONJ) in the French national pharmacovigilance database (from 1985 to 2013). To evaluate notoriety bias, drug‐related risk factors for ONJ [as specified in the summary of product characteristics (SPC) of bisphosphonates] were systematically scanned for notifications of reports of ONJ occurring under bisphosphonate therapy. When a risk factor was present, the ONJ was considered as not due to bisphosphonates, and a second ROR was calculated under the hypothesis of maximum bias.ResultsIn total, 148 cases of ONJ were reported (143 with bisphosphonates and five without). The raw ROR was 3448 (95% confidence interval 1413–8417). After analysis of the reports, only 86 had no mention of a risk factor for ONJ. The ROR under the maximum bias hypothesis was 87 (95% confidence interval 63–121). Among ONJ where chemotherapy was being administered simultaneously to bisphosphonates, 27 reports did not consider the chemotherapy to be implicated, despite seven of these occurring in cases where ONJ was mentioned in the summary of product characteristics.ConclusionsThe existence of a notoriety bias has an impact on measures of disproportionality. The detection of pharmacovigilance signals might be delayed. It is advisable to list all drugs being taken when an adverse drug reaction occurs, and not only those known to be associated with the observed reaction. Copyright © 2014 John Wiley & Sons, Ltd.
Australian men with cancer practice complementary therapies (CTs) as a coping strategy
Authors: Klafke Nadja , Eliott Jaklin A. , Olver Ian N. , Wittert Gary A. 
Published: April 2014

AbstractObjectiveThe aim of this study was to explore how and why Australian men with cancer practice complementary therapies (CTs) and how their significant others (SOs) contribute to the regular uptake of CTs.MethodsThis qualitative study employed semi‐structured interviews with 26 male cancer patients and 24 SOs. Participants were purposefully sampled from a preceding Australian survey investigating the use of CTs in men with cancer (94% response rate and 86% consent rate for follow‐up interview). Interviews were conducted in a metropolitan location, and the 43 interview transcripts were analyzed thematically.ResultsThree core themes were identified: men used CTs as (a) problem‐focused coping (e.g., diet modification), (b) emotion‐focused coping (e.g., meditation), and (c) meaning‐based coping (e.g., prayer). Practicing CTs helped men to cope with physical, emotional, and spiritual concerns, although some men spoke of difficulties with practicing meditation to regulate their emotions. SOs were supportive of men's coping strategies but were only rarely involved in men's emotion‐focused coping.ConclusionsComplementary therapies have the potential to facilitate coping with cancer, independent of any measurable physiological benefit. Our findings suggest that when clinicians engage in conversations about CTs use, they should consider the type of coping strategy employed by their patient. Such information may enhance the efficacy of some interventions (e.g., meditation) and also provide for an opportunity to discuss patients' expectations concerning CTs. Copyright © 2014 John Wiley & Sons, Ltd.
Using the International Classification of Functioning, Disability and Health in Physiotherapy in Multidisciplinary Vocational Rehabilitation: A Case Study of Low Back Pain
Authors: Finger Monika E. , Selb Melissa , De Bie Robert , Escorpizo Reuben 
Published: April 2014

AbstractObjectivesMultidisciplinary teamwork is increasingly recognized as a critical factor of success in vocational rehabilitation. Although its clinical implementation is still challenging, the International Classification of Functioning, Disability and Health (ICF) has shown to be a useful framework to facilitate communication between stakeholders, to help structure rehabilitation plans and for setting goals and clarifying team roles. With this in mind, the objective of this teaching case study is to illustrate an application of ICF‐based tools in a multidisciplinary return to work (RTW) program for patients with non‐specific low back pain (NLBP) from the perspective of the physiotherapist.ParticipantThe participant is a 42‐year‐old kindergarten teacher, who was on sick leave for 10 weeks due to NLBP.MethodsThis case study describes the use of ICF‐based tools such as the Rehabilitation Management Sheet (RehabManagement‐Sheet) to guide the rehabilitation process and facilitate team‐based and physiotherapist goal setting and documentation in a multidisciplinary RTW program for NLBP.ResultsUtilizing ICF tools, we defined long‐term and short‐term goals, documented improvements in the patient's impairments, activity limitations, and participation restrictions and formulated action steps.ConclusionThe use of ICF‐based tools in multidisciplinary rehabilitation allows for a comprehensive assessment, common goal setting and coordinated intervention planning. ICF‐based tools like the RehabManagement‐Sheet support the physiotherapist's role within the rehabilitation team by enhancing transparency in goal setting and intervention planning across disciplines. Copyright © 2014 John Wiley & Sons, Ltd.
Investigation of ISFET device parameters to optimize for impedimetric sensing of cellular adhesion
Authors: Susloparova Anna , Vu Xuan Thang , Koppenhöfer Dieter , Law Jessica Ka‐Yan , Ingebrandt Sven 
Published: April 2014

In this study we describe how we were able to use an equivalent‐electrical circuit for the cell–transistor contact to optimize a future chip design for better performance in cell–substrate adhesion experiments. From our simulations we found that higher capacitances of the source and drain contact lines are able to shift the cell adhesion effects to more moderate frequencies in the range of up to 200 kHz. For larger cell–substrate adhesion effects, the transconductance value of the FET devices needs to be increased as well. We used these simulation results to design a new generation of FET devices. These devices were successfully fabricated in our clean room facilities. In electronic cell–substrate adhesion experiments this new generation of devices showed superior performance compared to an earlier version. Most importantly, our new devices have now an almost flat topology enabling future single‐cell migration experiments. HEK293 cells adhered with a flat morphology to a newly fabricated FET sensor device (scale bar 50 µm)
The change of crystallite sizes and magnetocaloric effect in rapidly quenched dysprosium
Authors: Zvonov A. I. , Pankratov N. Yu. , Karpenkov D. Yu. , Smarzhevskaya A. I. , Karpenkov A. Yu. , Nikitin S. A. 
Published: April 2014

AbstractSynthesis of nanocrystalline and microcrystalline dysprosium was carried out. Temperature and field dependences of magnetocaloric effect were performed by direct method in field up to 1.2 T and temperature range 80‐190 K. It was found that the nanocrystalline state leads to a significant reduction of the magnetic phase transition temperatures and to a small increase of the maximum value of the magnetocaloric effect in dysprosium. It is associated with the large amount of interface atoms in nanocrystalline Dy ribbons with a lower number of the nearest‐neighbor atoms, causing the magnetic phase transition temperature decrease. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Competition between FFLO and BCS superconducting states in clean asymmetrical ferromagnet‐superconductor structures
Authors: Proshin Yurii N. , Khusainov Marat M. , Minnullin Arthur 
Published: April 2014

AbstractThe theory of proximity effect, based on the boundaryvalue problem for the Eilenberger function in view of the in‐plane Fulde‐Ferrell‐Larkin‐Ovchinnikov (FFLO) states, is proposed for thin asymmetrical structures FS, where F is a ferromagnetic metal and S is a BCS superconductor. The dependencies of critical temperature on an exchange field of the F metal, electronic correlations in the S and F metals, and thicknesses of layers F and S are calculated for four‐layered FS systems and FS superlattices. A proposed classification of states includes up to 8 different states which are characterized by phase shifts between superconducting order parameters for neighboring S(F) layers and mutual orientation of magnetizations in adjacent F layers. For asymmetrical FS systems the solitary reentrant superconductivity is predicted. It is shown that the 2D‐FFLO state prevails over the BCS one on the solitary peaks wings. The real candidate for observing predicted phenomena is Gd/La system, for which we found the sign and value of the constant of electronelectron interaction in gadolinium and explain the experimentally observed absence of the suppression of three dimensional superconductivity for symmetrical Gd/La superlattice. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)