bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation and properties of tannic acid cross‐linked collagen scaffold and its application in wound healing
Authors: Natarajan Venkatachalam , Krithica Natarajan , Madhan Balaraman , Sehgal Praveen Kumar 
Published: December 2012

AbstractA biodurable porous scaffold of collagen with good biocompatibility and enhanced wound healing potential is prepared through casting technique using tannic acid (TA) as crosslinker. The morphological analysis of the tannic acid cross‐linked collagen scaffold (TCCs) distinctively shows scaly interlinks with large pores. The enzymatic stability of the scaffold is characterized in vitro to detail the role of TA in stabilization of collagen matrix against collagenolytic degradation. TCCs shows more stability (>54%) against collagenase than that of the collagen scaffolds (Cs). The attenuated total reflectance Fourier transform infrared analysis of the TCCs confirms the noncovalent interaction between collagen and TA. The biocompatibility of the scaffold (TCCs) in vitro has been established using 3T3 fibroblasts. Therapeutic and wound healing potential of the TCCs has been studied in vivo using excision wound model in rats. The results clearly indicates that the TCCs has greater and significant effect in wound closure and increased the wound healing rate compared with native Cs. This biocompatible and biodurable scaffold may find broad applications in the tissue engineering and drug delivery applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Treatment of melasma with the 1,927‐nm fractional thulium fiber laser: A retrospective analysis of 20 cases with long‐term follow‐up
Authors: Niwa Massaki Ane Beatriz M. , Eimpunth Sasima , Fabi Sabrina G. , Guiha Isabella , Groff William , Fitzpatrick Richard 
Published: December 2012

AbstractBackgroundMelasma is a common acquired symmetrical hyperpigmentation that is often recurrent and refractory.ObjectivesTo investigate the efficacy and safety of a single administration of high‐density fractional thulium fiber laser (1,927 nm) for the treatment of refractory melasma in 20 patients.Materials and MethodsA retrospective chart and photographs review of 20 women (Fitzpatrick skin type II–IV) with clinical diagnosis of melasma treated with the 1,927‐nm fractionated thulium laser at 10 or 20 mJ/cm2, with 60–70% surface area coverage. Four investigators independently evaluated Melasma Area Severity Index (MASI) scores before, 4 weeks, 3–6 months, and 6–12 months after treatment.ResultsMean MASI scores decreased dramatically from 13.2 ± 5.4 before treatment to 8.5 ± 3.5 at 4 weeks after laser treatment (P = 0.004). Patient assessment revealed that 12 of the 20 subjects had more than 50% clearance of their melasma. Recurrence was reported by 7 out of 15 patients who were successfully followed‐up (mean 10.2 months). Two patients developed postinflammatory hyperpigmentation that subsided with topical bleaching after 3 months.ConclusionHigh‐density coverage fractional 1,927‐nm thulium laser proved to be safe and effective for melasma with long‐term remission. Lasers Surg. Med. 45: 95–101, 2013. © 2012 Wiley Periodicals, Inc.
Topical perfluorodecalin resolves immediate whitening reactions and allows rapid effective multiple pass treatment of tattoos
Authors: Reddy Kavitha K. , Brauer Jeremy A. , Anolik Robert , Bernstein Leonard , Brightman Lori , Hale Elizabeth , Karen Julie , Weiss Elliot , Geronemus Roy G. 
Published: December 2012

AbstractBackground and ObjectiveLaser tattoo removal using multiple passes per session, with each pass delivered after spontaneous resolution of whitening, improves tattoo fading in a 60‐minute treatment time. Our objective was to evaluate the safety and efficacy of topical perfluorodecalin (PFD) in facilitating rapid effective multiple‐pass tattoo removal.Study DesignIn a randomized, controlled study using Q‐switched ruby or Nd:YAG laser, 22 previously treated tattoos were treated with 3 passes using PFD to resolve whitening after each pass (“R0 method”). In previously untreated symmetric tattoos, seven were treated over half of the tattoo with the R20 method, and the opposite half with 4 passes using PFD (R0 method); two were treated over half with a single pass and the opposite half with 4 passes using PFD (R0 method); and six treated over half with a single pass followed by PFD and the opposite half with a single pass alone. Blinded dermatologists rated tattoo fading at 1–3 months. Optical coherence tomography (OCT) imaging of whitening was performed in two tattoos.ResultsTopical PFD clinically resolved immediate whitening reactions within a mean 5 seconds (range 3–10 seconds). Tattoos treated with the R0 method demonstrated excellent fading in an average total treatment time of 5 minutes. Tattoo areas treated with the R0 method demonstrated equal fading compared to the R20 method, and improved fading compared to a single pass method. OCT imaging of whitening demonstrated epidermal and dermal hyper‐reflective “bubbles” that dissipated until absent at 9–10 minutes after PFD application, and at 20 minutes without intervention.ConclusionsMultiple‐pass tattoo removal using PFD to deliver rapid sequential passes (R0 method) appears equally effective as the R20 method, in a total treatment time averaging 5 minutes, and more effective than single pass treatment. OCT‐visualized whitening‐associated “bubbles,” upon treatment with PFD, resolve twice as rapidly as spontaneous resolution. Lasers Surg. Med. 45: 76–80, 2013. © 2012 Wiley Periodicals, Inc.
PKM2 inhibitor shikonin suppresses TPA‐induced mitochondrial malfunction and proliferation of skin epidermal JB6 cells
Authors: Li Wenjuan , Liu Joan , Zhao Yunfeng 
Published: December 2012

AbstractChemoprevention has been a pivotal and effective strategy during the skin cancer treatment. Using human skin normal and tumor samples, we demonstrated that both the expression and activity levels of pyruvate kinase M2 (PKM2) were higher in skin tumor tissues than normal tissues, suggesting that PKM2, one of important metabolic enzyme, might serve as a target for skin cancer prevention and/or therapy. Shikonin, a small‐molecule active chemical, has been studied as an anti‐cancer drug candidate in human cancer models. However, the mechanism of action and the chemopreventive potential of shikonin are unclear. Herein, we used the skin epidermal JB6 P+ cells and demonstrated that shikonin suppressed the tumor promoter 12‐O‐tetradecanoylphorbol 13‐acetate (TPA) induced neoplastic cell transformation and PKM2 activation in the early stage of carcinogenesis. Mitochondrial functions were inhibited by TPA treatment, as indicated by reduced mitochondrial membrane potential and mitochondrial respiration, which were restored by shikonin. We also examined the levels of lactate as a glycolysis marker, and shikonin suppressed its increase caused by tumor promoter treatment. Modulation of cell metabolism by shikonin was associated with G2–M phase accumulation, and Fra‐1 (a major subunit of activator protein 1 in skin tumorigenesis) downregulation. In addition, we demonstrated that AMP‐activated protein kinase (AMPK), an energy sensor, which is inactivated by TPA, shikonin could reverse AMPK activity. These results suggest that shikonin bears chemopreventive potential for human skin cancers in which PKM2 is upregulated, which might be mediated by inhibiting oncogenic activation, PKM2 activation, and mitochondrial dysfunction. © 2012 Wiley Periodicals, Inc.
IL‐32γ enhances TNF‐α‐induced cell death in colon cancer
Authors: Park Eun‐Seok , Yoo Jae‐Myung , Yoo Hwan‐Soo , Yoon Do‐Young , Yun Yeo‐Pyo , Hong JinTae 
Published: December 2012

AbstractInterleukin (IL)‐32 is a recently discovered cytokine that appears to play an important role in human colon cancer growth. We investigated that IL‐32γ in combination with TNF‐α remarkably inhibited cell growth of human colon cancer cells (HCT116 and SW620) and tumor growth in xenograft‐bearing nude mice. The transient enforced overexpression of IL‐32γ potentiated the inhibitory effect of TNF‐α on DNA synthesis, cell number and protein content, and enhanced apoptosis in colon cancer cells. We also found that knockdown of IL‐32γ by siRNA showed the abolishment of cell growth inhibitory effect of TNF‐α. The IL‐32γ‐overexpressing colon cancer cells further increased TNF‐α‐mediated expression of p38 MAPK as well as that of Bax, cleaved caspase‐3 and ‐9, but decreased that of antiapoptotic proteins such as Bcl‐2, cellular inhibitor of apoptosis protein (IAP) and X chromosome IAP. In xenograft model, the lipopolysaccharide (LPS)‐injected (1.25 mg/kg) mice inoculated with IL‐32γ‐transfected HCT116 colon cancer cells were more decrease tumor volume and weight than inoculated with vector. Tumor tissues isolated from LPS‐injected mice inoculated with IL‐32γ‐overexpressing colon cancer cells potentiated the expression levels of pro‐apoptotic proteins such as cleaved caspase‐3, 9 and Bax, but decreased that of Bcl‐2. Furthermore, the mice increased IL‐10 production, but decreased IL‐6 levels in serum. In conclusion, our results suggest that IL‐32γ may potentiate TNF‐α‐induced cell growth inhibition through activation of p38 MAPK pathways.
Utility of multi‐channel surface electromyography in assessment of focal hand dystonia
Authors: Sivadasan Ajith , Sanjay M. , Alexander Mathew , Devasahayam Suresh R. , Srinivasa Babu K. 
Published: December 2012

ABSTRACTIntroductionSurface electromyography (SEMG) allows objective assessment and guides selection of appropriate treatment in focal hand dystonia (FHD).MethodsSixteen‐channel SEMG obtained during different phases of a writing task was used to study timing, activation patterns, and spread of muscle contractions in FHD compared with normal controls. Customized software was developed to acquire and analyze EMG signals.ResultsSEMG of FHD subjects (20) showed “early onset” during motor imagery, rapid proximal muscle recruitment, agonist‐antagonist co‐contraction involving proximal muscle groups, “delayed offset” after stopping writing, higher rectified mean amplitudes, and mirror activity in contralateral limb compared with controls (16). Muscle activation latencies were heterogenous in FHD.ConclusionsAnticipation, delayed relaxation, and mirror EMG activation were noted in FHD. A clear pattern of muscle activation cannot be ascertained. Multi‐channel SEMG can aid in objective assessment of temporal‐spatial distribution of activity and can refine targeted therapies like chemodenervation and biofeedback. Muscle Nerve 48: 415–422, 2013
WEB‐spline based mesh‐free finite element analysis for the heat equation and the time‐dependent Navier–Stokes equation: A survey
Authors: Chaudhary Sudhakar , Srinivas Kumar V.V.K. 
Published: December 2012

AbstractIn this article, we give some numerical techniques and error estimates using web‐spline based mesh‐free finite element method for the heat equation and the time‐dependent Navier–Stokes equations on bounded domains. The web‐spline method uses weighted extended B‐splines on a regular grid as basis functions and does not require any grid generation. We demonstrate the method by providing numerical results for the Poisson's and stationary Stokes equation. © 2012 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2013
An association of craniopharyngioma in Turner syndrome
Authors: Farooque Aqeel , Atapattu Navoda , Amarasena Sujeewa , Högler Wolfgang , English Martin W. , Kirk Jeremy M.W. 
Published: December 2012

AbstractTurner syndrome (TS) (approximately 1:5,000 births) and craniopharyngioma (CP) (1:50,000 children) are both rare conditions. We present three cases of TS with CP, an association not previously described. Visual failure, poor growth or headache led to MRI diagnosis of CP. Whilst two had evidence of hypopituitarism at diagnosis of CP, they all developed hypopituitarism following surgical debulking. Two required radiotherapy due to regrowth. Whether CP and TS share a similar aetiology is unknown. Clinicians need to be aware of this association, and should perform urgent MRI scanning in TS patients with headache, visual impairment or clinical/biochemical evidence of hypopituitarism. Pediatr Blood Cancer 2013; 60: E7–E9. © 2012 Wiley Periodicals, Inc.
Children's Oncology Group's 2013 blueprint for research: Nursing discipline
Authors: Landier Wendy , Leonard Marcia , Ruccione Kathleen S. 
Published: December 2012

AbstractIntegration of the nursing discipline within cooperative groups conducting pediatric oncology clinical trials provides unique opportunities to maximize nursing's contribution to clinical care, and to pursue research questions that extend beyond cure of disease to address important gaps in knowledge surrounding the illness experience. Key areas of importance to the advancement of the nursing discipline's scientific knowledge are understanding the effective delivery of patient/family education, and reducing illness‐related distress, both of which are integral to facilitating parental/child coping with the diagnosis and treatment of childhood cancer, and to promoting resilience and well‐being of pediatric oncology patients and their families. Pediatr Blood Cancer 2013; 60: 1031–1036. © 2012 Wiley Periodicals, Inc.
Children's Oncology Group's 2013 blueprint for research: Behavioral science
Authors: Noll Robert B. , Patel Sunita K. , Embry Leanne , Hardy Kristina K. , Pelletier Wendy , Annett Robert D. , Patenaude Andrea , Lown E. Anne , Sands Stephen A. , Barakat Lamia P. ,   On behalf of the COG Behavioral Sciences Committee
Published: December 2012

AbstractBehavioral science has long played a central role in pediatric oncology clinical service and research. Early work focused on symptom relief related to side effects of chemotherapy and pain management related to invasive medical procedures. As survival rates improved, the focused has shifted to examination of the psychosocial impact, during and after treatment, of pediatric cancer and its treatment on children and their families. The success of the clinical trials networks related to survivorship highlights an even more critical role in numerous domains of psychosocial research and care. Within the cooperative group setting, the field of behavioral science includes psychologists, social workers, physicians, nurses, and parent advisors. The research agenda of this group of experts needs to focus on utilization of psychometrically robust measures to evaluate the impact of treatment on children with cancer and their families during and after treatment ends. Over the next 5 years, the field of behavioral science will need to develop and implement initiatives to expand use of standardized neurocognitive and behavior batteries; increase assessment of neurocognition using technology; early identification of at‐risk children/families; establish standards for evidence‐based psychosocial care; and leverage linkages with the broader behavioral health pediatric oncology community to translate empirically supported research clinical trials care to practice. Pediatr Blood Cancer 2013; 60: 1048–1054. © 2012 Wiley Periodicals, Inc.
Children's Oncology Group's 2013 blueprint for research: Rare tumors
Authors: Rodriguez‐Galindo Carlos , Krailo Mark , Frazier Lindsay , Chintagumpala Murali , Amatruda James , Katzenstein Howard , Malogolowkin Marcio , Spector Logan , Pashankar Farzana , Meyers Rebecka , Tomlinson Gail ,   on behalf of the COG Rare Tumors Disease Committee
Published: December 2012

AbstractIn the US, approximately 2,000 children are diagnosed with rare cancers each year, with 5‐year survival ranging from <20% for children with advanced carcinomas to >95% for children with intraocular retinoblastoma or localized germ cell tumors. During the last years, 12 clinical studies have been successfully completed in children with retinoblastoma, liver tumors, germ cell tumors, and infrequent malignancies, including therapeutic, epidemiologic, and biologic studies. Current efforts are centered in the development of large international collaborations to consolidate evidence‐based definitions and risk stratifications that will support international Phase 3 clinical trials in germ cell tumors, hepatoblastoma, and other rare cancers. Pediatr Blood Cancer 2013; 60: 1016–1021. © 2012 Wiley Periodicals, Inc.
Polymeric phthalates: Potential nonmigratory macromolecular plasticizers
Authors: Braslau Rebecca , Schäffner Friederike , Earla Aruna 
Published: December 2012

AbstractThe synthesis of 4‐vinyl‐1,2‐phthalate esters via Suzuki coupling is described, followed by nitroxide‐mediated polymerization to prepare short homopolymers (degree of polymerization [DP] = 10–40, polydispersity index [PDI] = 1.1–1.3). Random copolymers with n‐butyl acrylate (NBA) were prepared. Copolymers rich in phthalate ester residues of medium lengths (DP = 16–48, PDI = 1.2–1.8) and of shorter lengths (DP = 8–17, PDI = 1.2–1.3) were prepared. Copolymers rich in NBA residues were also prepared (DP = 13–19, PDI = 1.2–1.3). All polymers were oily liquids, with glass transitions temperatures undetected between 75 and −40 °C, indicating these polymeric phthalates hold promise as potential nonmigratory phthalate plasticizers. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Copolymerization of ethylene with 1,1‐disubstituted olefins catalyzed by ansa‐(fluorenyl)(cyclododecylamido)dimethyltitanium complexes
Authors: Nakayama Yuushou , Sogo Yuuichi , Cai Zhengguo , Shiono Takeshi 
Published: December 2012

AbstractA series of titanium complexes with ansa‐(fluorenyl)(cyclododecylamido) ligands, Me2Si(η3‐R)(N‐c‐C12H23)TiMe2 [R = fluorenyl (5), 2,7‐tBu2fluorenyl (6), 3,6‐tBu2fluorenyl (7)], was synthesized. The crystal structure of complex 6 revealed η3‐coordination of the fluorenyl moiety to the metal. Upon activation with trialkylaluminum‐free modified methylaluminoxane, complexes 5–7 as well as the corresponding tBu amide complexes, Me2Si(η3‐R)(NtBu)TiMe2 [R = fluorenyl (2), 2,7‐tBu2fluorenyl (3), 3,6‐tBu2fluorenyl (4)], were adopted as the catalysts for the copolymerization of ethylene (E) and isobutylene (IB). Among these complexes, complex 6 was found to achieve the highest IB incorporation to produce alternating E‐IB copolymers. Complex 6 system also achieved copolymerization of E and limonene. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Convenient synthesis of cyclic carbonates from CO2 and epoxides by simple secondary and primary ammonium iodides as metal‐free catalysts under mild conditions and its application to synthesis of polymer bearing cyclic carbonate moiety
Authors: Aoyagi Naoto , Furusho Yoshio , Endo Takeshi 
Published: December 2012

AbstractHydroiodides of secondary and primary amines effectively catalyzed the reaction of carbon dioxide and epoxides under mild conditions such as ordinary pressure and ambient temperature, to obtain the corresponding five‐membered cyclic carbonates in moderate to high yields. Detailed investigation showed that the catalytic activity was highly affected by the counter anions of the ammonium salts; the iodides catalyzed efficiently the carbonate‐forming reactions, whereas the bromide and chloride counterparts exhibited almost no catalysis. We also revealed that two important factors on the amine moieties that affected the catalytic reactions. First, the catalytic activity increased with increasing bulkiness of the substituents on the ammonium nitrogen atoms. Second, the catalysis became more efficient as the parent amines become more basic. Dicyclohexylammonium iodide was the best catalyst among the ammonium salts investigated in this study. As an application of this reaction system, we synthesized homo‐ and copolymers bearing epoxide pendant groups as substrates, which were converted with high efficiency into the corresponding homo‐ and copolymers bearing cyclic carbonate pendant groups under 1 atm at 45 °C. All polymers were easily purified simply by precipitation in water, and were isolated in high yields (>95%). © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Structure of polymer and particle aggregates in hydrogel composites
Authors: Berts Ida , Gerelli Yuri , Hilborn Jöns , Rennie Adrian R. 
Published: December 2012

AbstractKnowledge of the structure of a biomaterial is usually vital to control its function. This article provides a structural characterization of a hyaluronan scaffold that has demonstrated good biocompatibility and is used to induce bone regeneration. Hyaluronan hydrogels are appealing materials that can function as a matrix to incorporate both organic and inorganic substances to enhance tissue growth. Because of the intrinsic properties of this swollen matrix, one needs a very sensitive technique that can be applied in situ to determine the organization of the polymers in a gel. Small‐angle neutron scattering is used to determine the characteristics of the inhomogeneous structure of the hydrogel both with and without added particles. The results are interpreted using models of structure with two length scales that are beyond the traditional picture of homogeneous gels. The observed structure and the dimensions can explain the previously reported rheological properties of gels containing different amount of polymers. Hydroxyapatite nanoparticles added to the gel are frozen in the gel matrix. We are able to determine the distribution and shape of these particles as they aggregate around the polymer chains. We have also concluded, in this case, that the particle structure is concentration independent. Information about the nanostructure for an applicable biomaterial guides the formulation, preparation, and use that should lead to further understanding of its exploitation. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2013
Prediction of Amine‐Based Liquid Rocket Propellant Shelf Life
Authors: Mohammadi Kaveh , Gorji Mahmoud 
Published: December 2012

AbstractThe storage stability of amine based rocket fuel called Samine was studied over 9 months for the first time. It was found that storage stability significantly depends on fuel oxidation with the air trapped in the storage tanks. A reduction of the triethylamine concentration from 50.2 % (wt) to less than 48 % was taken as the end of shelf life of the propellant. After obtaining experimental data from accelerated storage stability tests, the shelf life of Samine was estimated using the Arrhenius equation. According to the kinetic studies, the oxidation reaction of fuel was a zero order reaction and the shelf lives of Samine in stainless‐steel tanks at 20 °C, 25 °C, and 30 °C were obtained to be 5.7, 3.7, and 2.4 years, respectively. Additionally, it was observed that for long time storage of amine based fuels like Samine it is better to use stainless‐steel or aluminum tanks, which are charged with an inert gas like nitrogen.