bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between obesity and sleep disorder in the elderly: evidence from NHANES 2005–2018
Authors: Wang Weifei , Chen Zhong , Zhang Wenyuan , Yuan Rui , Sun Yaqi , Yao Qi , Lu Jian , Zheng Jungang 
Published: August 2024

BackgroundThe available data exhibit inconsistent findings regarding the association between obesity and sleep problems among older adults. The objective of this study was to assess the potential association between obesity and sleep disorders in the older population.MethodsThe data utilised in this cross-sectional investigation was obtained from the National Health and Nutritional Examination Survey (NHANES) conducted between 2005 and 2018. The study employed a multivariate logistic regression model and conducted subgroup analysis to evaluate the association between obesity and sleep disturbance.ResultsThe investigation consisted of 2,570 older people who provided complete information, out of which 324 individuals satisfied the criteria for sleep disturbance. The findings from the multivariable adjusted logistic regression model indicate that individuals in the overweight and normal weight groups exhibited decreased odds of experiencing sleep disorder, as evidenced by the adjusted odds ratios (AOR) of 0.46 (95% confidence interval [CI] = 0.34–0.61) and 0.33 (95% CI = 0.22–0.47), respectively. These results were statistically significant (p &lt; 0.001) when compared to individuals in the obese group. The investigation of age and gender subgroups demonstrated similar associations between various BMI categories and sleep disorders in the older population.ConclusionIn summary, there exists a correlation between obesity and sleep disorders in the senior population. A significant association was observed between BMI and the likelihood of experiencing sleep disorders, indicating a dose–response relationship. Individuals with a higher BMI demonstrated a heightened likelihood of experiencing sleep disorders compared to those with a lower BMI.
Sulforaphane regulates cell proliferation and induces apoptotic cell death mediated by ROS-cell cycle arrest in pancreatic cancer cells
Authors: Cho Yongmin , Park Moon Nyeo , Choi Min , Upadhyay Tarun Kumar , Kang Han Na , Oh Jeong Min , Min Soonki , Yang Ji-Ung , Kong Moonkyoo , Ko Seong-Gyu , Rahman Md Ataur , Harrath Abdel Halim , Kim Bonglee 
Published: August 2024

BackgroundPancreatic cancer (PC), sometimes referred to as pancreatic ductal adenocarcinoma (PDAC), is a major cause of global mortality from cancer. Pancreatic cancer is a very aggressive and devastating kind of cancer, characterized by limited options for therapy and low possibilities of survival. Sulforaphane (SFN), a naturally occurring sulfur-containing compound, is believed to possess anti-inflammatory, anti-obesity, and anti-cancer characteristics.ObjectiveHowever, efficient preventative and treatment measures are essential and SFN has been studied for its ability to suppress pancreatic cancer cell proliferation and induce apoptosis.MethodsHere, SFN induced cytotoxicity and apoptosis in PDAC cell lines such as MIA PaCa-2 and PANC-1 cells, as evaluated by cytotoxicity, colony formation, western blot analysis, fluorescence-activated cell sorting (FACS), reactive oxygen species (ROS) detection, caspase-3 activity assay, immunofluorescence assay, and mitochondrial membrane potential assay.ResultsIn MIA PaCa-2 and PANC-1 cells, SFN inhibited cell survival and proliferation in a dose-dependent manner. The activation of caspase zymogens results in cleaved PARP and cleaved caspase-3, which is associated with an accumulation in the sub G1 phase. Furthermore, SFN increased ROS level and γH2A.X expression while decreasing mitochondrial membrane potential (ΔΨm). Notably, the ROS scavenger N-Acetyl-L-cysteine (NAC) was shown to reverse SFN-induced cytotoxicity and ROS level. Subsequently, SFN-induced cell cycle arrest and apoptosis induction as a Trojan horse to eliminate pancreatic cancer cells via ROS-mediated pathways were used to inhibit pancreatic cancer cells.ConclusionCollectively, our data demonstrates that SFN-induced cell death follows the apoptosis pathway, making it a viable target for therapeutic interventions against pancreatic cancer.
A novel and apparent de novo ALAS2 missense variant associated with congenital sideroblastic anemia
Authors: Cai Jianling , Liu Tianming , Huang Yuxuan , Chen Hongxing , Yu Meidie , Zhang Dongqing , Huang Zhanqin 
Published: August 2024

BackgroundCongenital sideroblastic anemia (CSA) constitutes a group of inherited erythropoietic disorders. Some affect mainly or exclusively erythroid cells; other syndromic forms occur within multisystem disorders with extensive nonhematopoietic manifestations. In this study, we have performed clinical and molecular investigations on a 10-year-old boy suspected of having CSA.MethodsRoutine blood examination, peripheral blood and bone marrow smears, and serum iron tests were performed. Gene mutation analysis was conducted using whole-exome sequencing (WES) and the results were confirmed using Sanger sequencing. Furthermore, the functional impact of the identified variant was assessed/predicted with bioinformatics methods.ResultsThe patient presented with severe microcytic anemia (hemoglobin, 50 g/L), iron overload and ring sideroblasts in the bone marrow. Moreover, WES revealed the presence of a hemizygous missense variant in ALAS2 (c.1102C &gt; T), changing an encoded arginine to tryptophan (p. Arg368Trp). This variant was verified via Sanger sequencing, and neither of the parents carried this variant, which was suspected to be a de novo variant. Using in silico analysis with four different software programs, the variant was predicted to be harmful. PyMol and LigPlot software showed that the p. Arg368Trp variant may result in changes in hydrogen bonds. The patient was treated with vitamin B6 combined with deferasirox. After 6 months, the hemoglobin increased to 99 g/L and the serum ferritin decreased significantly.ConclusionWe report a novel pathogenic variant in the ALAS2 gene (c.1102C &gt; T:p. Arg368Trp), which caused CSA in a 10-year-old boy. Mutational analysis is important in patients with CSA when family history data are unavailable. Anemia due to the ALAS2 Arg368Trp variant responds to pyridoxine supplements.
Identification of biomarkers and potential drug targets in osteoarthritis based on bioinformatics analysis and mendelian randomization
Authors: Cheng Feng , Li Mengying , Hua Haotian , Zhang Ruikun , Zhu Yiwen , Zhu Yingjia , Zhang Yang , Tong Peijian 
Published: August 2024

BackgroundOsteoarthritis (OA) can lead to chronic joint pain, and currently there are no methods available for complete cure. Utilizing the Gene Expression Omnibus (GEO) database for bioinformatics analysis combined with Mendelian randomization (MR) has been widely employed for drug repurposing and discovery of novel therapeutic targets. Therefore, our research focus is to identify new diagnostic markers and improved drug target sites.MethodsGene expression data from different tissues of synovial membrane, cartilage and subchondral bone were collected through GEO data to screen out differential genes. Two-sample MR Analysis was used to estimate the causal effect of expression quantitative trait loci (eQTL) on OA. Through the intersection of the two, core genes were obtained, which were further screened by bioinformatics analysis for in vitro and in vivo molecular experimental verification. Finally, drug prediction and molecular docking further verified the medicinal value of drug targets.ResultsIn the joint analysis utilizing the GEO database and MR approach, five genes exhibited significance across both analytical methods. These genes were subjected to bioinformatics analysis, revealing their close association with immunological functions. Further refinement identified two core genes (ARL4C and GAPDH), whose expression levels were found to decrease in OA pathology and exhibited a protective effect in the MR analysis, thus demonstrating consistent trends. Support from in vitro and in vivo molecular experiments was also obtained, while molecular docking revealed favorable interactions between the drugs and proteins, in line with existing structural data.ConclusionThis study identified potential diagnostic biomarkers and drug targets for OA through the utilization of the GEO database and MR analysis. The findings suggest that the ARL4C and GAPDH genes may serve as therapeutic targets, offering promise for personalized treatment of OA.
The mechanisms and drug therapies of colorectal cancer and epigenetics: bibliometrics and visualized analysis
Authors: Tian Siyu , Chen Min 
Published: August 2024

BackgroundNumerous studies have demonstrated a link between epigenetics and CRC. However, there has been no systematic analysis or visualization of relevant publications using bibliometrics.Methods839 publications obtained from the Web of Science Core (WoSCC) were systematically analyzed using CiteSpace and VOSviewer software.ResultsThe results show that the countries, institutions, and authors with the most published articles are the United States, Harvard University, and Ogino and Shuji, respectively. SEPT9 is a blood test for the early detection of colorectal cancer. Vitamin D and gut microbiota mediate colorectal cancer and epigenetics, and probiotics may reduce colorectal cancer-related symptoms. We summarize the specific epigenetic mechanisms of CRC and the current existence and potential epigenetic drugs associated with these mechanisms. It is closely integrated with clinical practice, and the possible research directions and challenges in the future are proposed.ConclusionThis study reviews the current research trends and hotspots in CRC and epigenetics, which can promote the development of this field and provide references for researchers in this field.
Ovarian tissue autotransplantation improves longevity in mice
Authors: Ruhliada Nikolai N. 
Published: August 2024

In this study, we show the improvement in life longevity in an experimental mouse model after step-by-step autologous ovarian transplantation and compare its effects with exogenic transdermal estradiol usage. This has proven to be more efficient than “traditional” hormonal replacement therapy. Despite the high speed and effectiveness of estradiol replacement deficiency in blood by its oral or transdermal use, no significant increase in the life longevity of animals and possibly in women was noted. The function of the transplanted fragment is usually limited to 6–12 months. This is enough for oncofertility purposes, sometimes, but not for longevity improvement. We performed periodical tissue return (autologous transplantation), containing both the cortex and medulla in the experimental mouse model, which resulted in a statistically reliable improvement in longevity. Our experience indicates the important role of medullary ovarian factors in slowing the aging process in the body and increasing the life expectancy in the experiment. As shown, the transdermal estrogen supportive therapy for ovarian deficiency improves estrogen levels but causes much slower decreases in the follicle stimulating hormone (FSH) and luteinizing hormone (LH). Moreover, we attained the best longevity with step-by-step periodic ovarian autotransplantation, thus making “prosthetics” of ovarian function longer than it is preplanned physiologically [direct correlation between the levels of FSH and lifespan (r = 0.98)]. The experimental model we suggested could be projected to other mammals or humans as cortical transplantation provides the same results for reproduction restoration in mice and humans and even for hormone level normalization, but there is still a lack of information about anti-aging factors in the ovarian medulla and cortex. Hence, we consider that the most important factor for the anti-aging ovarian transplantation technology is to preserve and transfer both the medulla and cortex as parts of the whole ovary.
A theory on political violence in hybrid regimes: insights from Colombia
Authors: Cendales Andrés , Guerrero-Sierra Hugo , Oñate Laura 
Published: August 2024

IntroductionThis article presents a model of political competition in which political parties, through clientelism strategies, vie for control of legislative seats. Parties exercise political violence to prevent potential rivals from gaining power and threatening their position within the hybrid political regime. The theory suggests that the degree of political violence exerted by parties in hybrid regimes will increase (decrease) as they concentrate more (less) power in the legislature.MethodsUsing the methodology of analytical narratives, we examine the narrative on political violence in the Colombian political regime to identify key actors, strategies, information sets, and institutional changes. From these identified elements, we construct a theoretical model of political competition within the mathematical theory of games to explain the institutional changes highlighted in the narrative. Finally, we develop an econometric model to find statistical evidence supporting the predictions of the theoretical model derived from the narrative.ResultsThe narrative and the analytical narrative indicate that, in a hybrid regime, a higher degree of political power concentration causes a higher rate of political violence. The estimation of the Vector Auto-regression model allows us to analyze the response of the violence rate to a shock in the concentration index. Following an unexpected increase in the concentration of political power, the violence rate shows an initial increase of approximately 0.3 percentage points above its initial value. Subsequently, the effect attenuates slightly and stabilizes at around 0.2 percentage points above the baseline, maintaining this level throughout the period.Discussion/ConclusionThe theoretical model proposed in this paper suggests an explanation of political violence that diverges from the explanations offered by traditional theories. We suggest that the theoretical model proposed here captures the historical logic of the relationship between violence, political clientelism, and exclusion in Colombia, a country with a relatively long tradition as a formal democracy (since 1958), leading us to interesting conclusions that have not been proposed so far in the literature on violence in Latin America.
The mediating role of perceived emotional intelligence: examining the impact of affective job satisfaction on organizational identification among Chinese technological workers
Authors: Lai Yuege , Gao Ge , Du Baiyan 
Published: August 2024

IntroductionBreaking new ground in the exploration of workplace dynamics, this study pioneers an investigation into the mediating role of perceived emotional intelligence (PEI) in the relationship between affective job satisfaction and organizational identification among Chinese technological workers. This novel focus addresses a critical gap in existing research, particularly in understanding the psychological underpinnings within this specific cultural and professional context.MethodsInvolving 392 workers aged 23 to 60, our research offers a comprehensive examination of how the three subdimensions of PEI - attention, clarity, and emotional repair - interact with job satisfaction to influence organizational identification. Through extensive questionnaires, we assessed these subdimensions alongside affective job satisfaction and the workers’ identification with their organization.ResultsOur findings reveal a significant, positive correlation between job satisfaction and all PEI subdimensions. Notably, while emotional clarity and emotional repair showed a positive relationship with organizational identity, attention did not. Further analysis highlighted the substantial direct impact of Affective Job Satisfaction on Organizational Identification, with emotional clarity and emotional repair playing critical mediating roles.DiscussionThese insights illuminate the unique function of Perceived Emotional Intelligence as a mediator and enhancer in the relationship between job satisfaction and organizational commitment. The results underscore the necessity of integrating strategies to cultivate emotional intelligence in the workplace, potentially leading to stronger organizational ties and improved overall worker well-being. By shedding light on these complex psychological mechanisms, our study not only enriches the theoretical landscape but also offers practical guidance for fostering healthier, more productive work environments.
Effects of work-family conflict, social support and burnout on job satisfaction among primary care physicians in Huaihai economic zone
Authors: Wen Zongliang , Wu Shenqin , Bai Long , Jintao Xu , Zhao Yun , Fang Jinhua , Abdirizak Jama Hamdi 
Published: August 2024

BackgroundPrimary care physicians (PCPs) are doctors in primary health care institutions, namely village clinics, township health centers and community health service centers (stations) who are the main providers of primary health care services in primary health care settings. Improving the overall health status of the population requires the support of a large number of primary care physicians; however, the job satisfaction of this group has not been sufficiently emphasized and recognized.ObjectiveThe purpose of this study was to examine the effects of primary care physicians’ work-family conflict on their job satisfaction, as well as the mediating role of burnout and the moderating role of social support.MethodsThis cross-sectional study was conducted from February 2023 to March 2023. Participants were 749 primary care physicians from four cities of Xuzhou, Linyi, Huaibei, and Shangqiu in the Huaihai Economic Zone of China. SPSS statistical analysis was used to evaluate the relationship between work-family conflict, social support, burnout and job satisfaction among medical workers.ResultsWork–family conflict had a significant negative effect on job satisfaction (β = −0.36, p&lt; 0.001), after adding burnout in the model, work–family conflict also negatively predicted job satisfaction (β = −0.32, p&lt; 0.001). Social support had a moderating effect on the direct effect of burnout on job satisfaction (β = 0.00, t = 2.66, p&lt; 0.01, 95%CI [0.001, 0.007]), the predictive effect of burnout on job satisfaction at high level of social support (β = −0.45, p&lt; 0.001) was higher than a low level of social support (β = −0.33, p&lt; 0.001).ConclusionsThis study demonstrated the negative impact of work-family conflict on primary care physicians’ job satisfaction, as well as the mediating role of burnout and the moderating role of social support on burnout and job satisfaction, which are important for improving primary care physicians’ job satisfaction and enhancing the quality of primary care in the future.
Prevalence and associated factors of insomnia symptoms among doctors and nurses under the context of high prevalence of multiple infectious diseases: a cross-sectional study
Authors: Chen Xu , Wang Bin , Wang Yu , He Surui , Liu Dongmei , Yan Dongmei 
Published: August 2024

IntroductionInsomnia symptoms are prevalent among healthcare workers and represent a potential public health problem. However, there is currently insufficient evidence on insomnia symptoms among doctors and nurses under the context of high prevalence of multiple infectious diseases after the pandemic in China. The purpose of this study was to analyze the prevalence of insomnia symptoms among doctors and nurses in third-grade class-A general hospitals under the context of high prevalence of multiple infectious diseases, and to explore the influence of demographic characteristics, work-related factors, health and lifestyle-related factors on insomnia symptoms.MethodsAn institution-based cross-sectional survey was conducted among doctors and nurses in two third-grade class-A general hospitals. A structured questionnaire was used to collect information on demographic characteristics, work-related factors, health and lifestyle-related factors, and insomnia symptoms among doctors and nurses. Multivariate logistics regression analysis was applied to identify factors significantly associated with insomnia symptoms among doctors and nurses, respectively.ResultsA total of 1,004 participants were included in this study, including 503 doctors and 501 nurses. The prevalence of insomnia symptoms in doctors and nurses was 47.7 and 51.3%, respectively. Multivariate logistics regression analysis showed that workplace violence (OR: 1.631, 95% CI: 1.050–2.532), doctor-patient relationship (OR: 1.603, 95% CI: 1.049–2.450), chronic pain (OR: 4.134, 95% CI: 2.579–6.625), chronic disease (OR: 1.825, 95% CI: 1.164–2.861), and anxiety symptoms (OR: 2.273, 95% CI: 1.357–3.807) were associated factors with insomnia symptoms in doctors. Education (OR: 0.301, 95% CI: 0.106–0.851), service years (OR: 1.978, 95% CI: 1.304–3.002), weekly working hours (OR: 1.694, 95% CI: 1.061–2.705), chronic pain (OR: 5.359, 95% CI: 3.241–8.860), and anxiety symptoms (OR: 2.472, 95% CI: 1.478–4.136) were associated factors with insomnia symptoms in nurses.ConclusionThe prevalence of insomnia symptoms among doctors and nurses was high, and affected by many factors. This information can inform tailored interventions to insomnia symptoms by doctors and nurses who play an important role in public health.
The differential role of socioeconomic status dimensions in depressive symptoms of aging adults: data from the Hamburg City Health cohort Study
Authors: Klimesch Anne , Ascone Leonie , Schmager Axel , Petersen Elina , Hoven Hanno , von dem Knesebeck Olaf , Gallinat Jürgen , Kühn Simone 
Published: August 2024

BackgroundSocioeconomic status (SES) has consistently been associated with depressive symptoms, however, it remains unclear which subset of SES variables is most relevant to the development of depressive symptoms. This study determined a standardized SES-Index to test the relationship of its sub-dimensions with depressive symptoms.MethodsHCHS data (N = 10,000; analysis sample n = 8,400), comprising participants 45+ years of age, was used. A standardized approach to quantify SES was employed. Depressive symptoms were quantified using the Patient Health Questionnaire-9 (PHQ-9). Using multiple linear regression models, PHQ-9-scores were modeled as a function of age and sex, and (1a) total SES-Index score versus (1b) its three sub-dimension scores (education, occupational status, income). Models were compared on explained variance and goodness of fit. We determined risk ratios (RR, concerning a PHQ-9 sum score ≥ 10) based on (low, middle, high; 2a) SES-Index scores and (2b) the sub-dimension scores, with groups further differentiated by sex and age (45–64 versus 65+). We distinguished between the total SES-Index score and its three sub-dimension scores to identify relevant SES sub-dimensions in explaining PHQ-9-variability or risk of depression.ResultsAmong all regression models (total explained variance 4–6%), income explained most variance, but performance of the SES-Index was comparable. Low versus high income groups showed the strongest differences in depressive trends in middle-aged females and males (RRs 3.57 and 4.91). In older age, this result was restricted to females (RR ≈ 2). Middle-aged males (versus females) showed stronger discrepancies in depressive trends pertaining to low versus high SES groups. In older age, the effect of SES was absent. Education was related to depressive trends only in middle-aged females and males. In an exploratory analysis, marital status and housing slightly increased model fit and explained variance while including somatic symptoms lead to substantial increases (R2adj = 0.485).ConclusionIn line with previous research, the study provides evidence for SES playing a significant role in depressive symptoms in mid to old age, with income being robustly linked to depressive trends. Overall, the relationship between SES and depressive trends appears to be stronger in males than females and stronger in mid compared to old age.