bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein analysis by desorption electrospray ionization mass spectrometry
Authors: Venter Andre R. 
Published: July 2024

AbstractThis review presents progress made in the ambient analysis of proteins, in particular by desorption electrospray ionization‐mass spectrometry (DESI‐MS). Related ambient ionization techniques are discussed in comparison to DESI‐MS only to illustrate the larger context of protein analysis by ambient ionization mass spectrometry. The review describes early and current approaches for the analysis of undigested proteins, native proteins, tryptic digests, and indirect protein determination through reporter molecules. Applications to mass spectrometry imaging for protein spatial distributions, the identification of posttranslational modifications, determination of binding stoichiometries, and enzymatic transformations are discussed. The analytical capabilities of other ambient ionization techniques such as LESA and nano‐DESI currently exceed those of DESI‐MS for in situ surface sampling of intact proteins from tissues. This review shows, however, that despite its many limitations, DESI‐MS is making valuable contributions to protein analysis. The challenges in sensitivity, spatial resolution, and mass range are surmountable obstacles and further development and improvements to DESI‐MS is justified.
Patient Experience and Feasibility of a Remote Monitoring System in Parkinson's Disease
Authors: Maas Bart R. , Speelberg Daniël H.B. , de Vries Gert‐Jan , Valenti Giulio , Ejupi Andreas , Bloem Bastiaan R. , Darweesh Sirwan K.L. , de Vries Nienke M. 
Published: July 2024

AbstractBackgroundRemote monitoring systems have the potential to measure symptoms and treatment effects in people with Parkinson's disease (PwP) in the home environment. However, information about user experience and long‐term compliance of such systems in a large group of PwP with relatively severe PD symptoms is lacking.ObjectiveThe aim was to gain insight into user experience and long‐term compliance of a smartwatch (to be worn 24/7) and an online dashboard to report falls and receive feedback of data.MethodsWe analyzed the data of the “Bringing Parkinson Care Back Home” study, a 1‐year observational cohort study in 200 PwP with a fall history. User experience, compliance, and reasons for noncompliance were described. Multiple Cox regression models were used to identify determinants of 1‐year compliance.ResultsWe included 200 PwP (mean age: 69 years, 37% women), of whom 116 (58%) completed the 1‐year study. The main reasons for dropping out of the study were technical problems (61 of 118 reasons). Median wear time of the smartwatch was 17.5 h/day. The online dashboard was used by 77% of participants to report falls. Smartphone possession, shorter disease duration, more severe motor symptoms, and less‐severe freezing and balance problems, but not age and gender, were associated with a higher likelihood of 1‐year compliance.ConclusionsThe 1‐year compliance with this specific smartwatch was moderate, and the user experience was generally good, except battery life and data transfer. Future studies can build on these findings by incorporating a smartwatch that is less prone to technical issues.
Clinical approach for managing patients with unexpected CDH1 mutations: A case report
Authors: Garitaonaindia Yago , Méndez Miriam , Valentín Fátima , Gutiérrez Lourdes , de Tejada Alberto Herreros , Sánchez Ruiz Antonio , Provencio Mariano , Romero Atocha 
Published: July 2024

AbstractBackgroundHereditary diffuse gastric cancer (HDGC) (OMIM# 137215) is an autosomal dominant cancer syndrome associated with CDH1 (OMIM# 192090) mutations. Prophylactic total gastrectomy (PTG) is the most recommended preventive treatment when a pathogenic mutation is found. However, the increasing use of genetic testing has led to the identification of incidental CDH1 mutations in individuals without a family history of gastric cancer. It remains unclear whether these patients should undergo prophylactic total gastrectomy.MethodsGermline DNA, obtained from peripheral blood, was analysed by NGS.ResultsA 47‐year‐old woman was diagnosed with high‐grade serous ovarian carcinoma, FIGO stage IIIC, with a Homologous Recombination Deficiency (HRD) GIS status of 78 (positive, cut‐off: 43). She received chemotherapy and niraparib treatment. A multigene panel test revealed no pathogenic mutations in BRCA1 (OMIM# 113705)/BRCA2 (OMIM# 600185) genes, but a de novo deletion of exon 16 in CDH1 was found incidentally. She had no previous family history of gastric or breast cancer. The patient was enrolled in a surveillance program involving periodic endoscopy and was diagnosed with diffuse gastric cancer through biopsies of a pale area in the antrum after 1 year of close endoscopic follow‐up.ConclusionThis case presents supportive evidence for the pathogenic classification of the loss of the last exon of CDH1.
Medial prefrontal cortex neurotransmitter abnormalities in posttraumatic stress disorder with and without comorbidity to major depression
Authors: Swanberg Kelley M. , Prinsen Hetty , Averill Christopher L. , Campos Leonardo , Kurada Abhinav V. , Krystal John H. , Petrakis Ismene L. , Averill Lynnette A. , Rothman Douglas L. , Abdallah Chadi G. , Juchem Christoph 
Published: July 2024

Posttraumatic stress disorder (PTSD) is a chronic psychiatric condition that follows exposure to a traumatic stressor. Though previous in vivo proton (1H) MRS) research conducted at 4 T or lower has identified alterations in glutamate metabolism associated with PTSD predisposition and/or progression, no prior investigations have been conducted at higher field strength. In addition, earlier studies have not extensively addressed the impact of psychiatric comorbidities such as major depressive disorder (MDD) on PTSD‐associated 1H‐MRS‐visible brain metabolite abnormalities. Here we employ 7 T 1H MRS to examine concentrations of glutamate, glutamine, GABA, and glutathione in the medial prefrontal cortex (mPFC) of PTSD patients with MDD (PTSD+MDD+; N = 6) or without MDD (PTSD+MDD−; N = 5), as well as trauma‐unmatched controls without PTSD but with MDD (PTSD−MDD+; N = 9) or without MDD (PTSD−MDD−; N = 18). Participants with PTSD demonstrated decreased ratios of GABA to glutamine relative to healthy PTSD−MDD− controls but no single‐metabolite abnormalities. When comorbid MDD was considered, however, MDD but not PTSD diagnosis was significantly associated with increased mPFC glutamine concentration and decreased glutamate:glutamine ratio. In addition, all participants with PTSD and/or MDD collectively demonstrated decreased glutathione relative to healthy PTSD−MDD− controls. Despite limited findings in single metabolites, patterns of abnormality in prefrontal metabolite concentrations among individuals with PTSD and/or MDD enabled supervised classification to separate them from healthy controls with 80+% sensitivity and specificity, with glutathione, glutamine, and myoinositol consistently among the most informative metabolites for this classification. Our findings indicate that MDD can be an important factor in mPFC glutamate metabolism abnormalities observed using 1H MRS in cohorts with PTSD.
The CCL2–CCR2 axis determines whether glomerular endocapillary hypercellularity or wire‐loop lesions develop through glomerular macrophage and neutrophil infiltration in lupus nephritis
Authors: Zoshima Takeshi , Baba Tomohisa , Nakatani Kimihiko , Nagata Michio , Mukaida Naofumi , Kawano Mitsuhiro 
Published: July 2024

AbstractThe CCL2–CCR2 axis is involved in lupus nephritis, however the precise roles in the mechanisms by which different pathological lesions develop after glomerular immune complex deposition remain elusive. Previously, we demonstrated that genetic CCR2 inhibition induced a histological switch from glomerular endocapillary hypercellularity to wire‐loop lesions in murine lupus nephritis. This study aimed to clarify the CCL2–CCR2 axis‐mediated cellular mechanism in the formation of these different pathological lesions. We injected MRL/lpr mouse‐derived monoclonal IgG3 antibody‐producing hybridomas, 2B11.3 or B1, into wild‐type (WT) mice to selectively induce glomerular endocapillary hypercellularity or wire‐loop lesions. The expression of chemokine and chemokine receptors was analyzed using RT‐quantitative PCR and/or immunofluorescence. We found 2B11.3 caused glomerular endocapillary hypercellularity in WT mice with glomerular infiltration of larger numbers of CCR2‐expressing macrophages and neutrophils phagocyting immune complex, whereas B1 induced wire‐loop lesions. In glomerular endocapillary hypercellularity, CCL2 was identified as the ligand involved in the CCR2‐positive cell infiltration; it was expressed by glomerular endothelial cells and macrophages. Notably, 2B11.3‐induced glomerular endocapillary hypercellularity converted to wire‐loop lesions with reduced glomerular macrophage and neutrophil infiltration in CCL2‐deficient (Ccl2−/−) mice similarly observed in Ccr2−/− mice. Moreover, this histological conversion was also observed when both glomerular macrophage and neutrophil infiltration were inhibited in anti‐Ly6G antibody‐treated Ccr5−/− mice but not when only glomerular macrophage infiltration was inhibited in Ccr5−/− mice or when only glomerular neutrophil infiltration was inhibited in anti‐Ly6G antibody‐treated WT mice. In contrast, B1 injection caused wire‐loop lesions in Ccl2−/− and Ccr2−/− mice, as observed in WT mice. Moreover, 2B11.3 induced CCL2 from glomerular endothelial cells to a larger extent than B1 when injected into Ccr2−/− mice. In conclusion, the CCL2–CCR2 axis determines whether glomerular endocapillary hypercellularity or wire‐loop lesions develop by regulating glomerular infiltration of phagocytic cells: macrophages and neutrophils. © 2024 The Pathological Society of Great Britain and Ireland.
Splenic complications in pediatric sickle cell disease: A retrospective cohort review
Authors: George Alex , Conneely Shannon E. , Mangum Ross , Fasipe Titilope , Lupo Philip J. , Scheurer Michael E. 
Published: July 2024

AbstractObjectiveTo delineate the natural history of splenic complications other than loss of splenic function in children with sickle cell disease (SCD), we performed a retrospective chart review of patients with SCD treated at the Texas Children's Hospital.MethodsWe determined the dates of diagnoses of splenic complications, the number of acute splenic sequestration crises (ASSC), and hydroxyurea treatment in pediatric patients with SCD. We also examined the association of hydroxyurea therapy with the onset and severity of ASSC.ResultsThe cumulative prevalence of splenic complications was 24.7% for splenomegaly, 24.2% for ASSC, 9.6% for hypersplenism, and 5.6% for splenectomy. The cumulative prevalence of splenic complications was highest in patients with hemoglobin Sβ0 (69.2%), intermediate in hemoglobin SS (33.3%), low in hemoglobin SC (9.0%), and non‐existent in hemoglobin Sβ+. The overall event rate of ASSC was 8.3 per 100 patient‐years. The event‐rate was 28.4 for hemoglobin Sβ0, 10.9 for hemoglobin SS, and 3.5 for hemoglobin SC. Patients with hemoglobin SS and hemoglobin Sβ0 on hydroxyurea therapy had a significantly higher occurrence of ASSC than those who were not, with event rates of 14.2 and 3.1, respectively. The event rate was also higher for children who started hydroxyurea before age 2 years than for those who started after this age (19.8 and 9.2, respectively).ConclusionsThe prevalence and severity of splenic problems vary widely between different sickle cell genotypes, with hemoglobin Sβ0 having the most severe complications. Hydroxyurea therapy is associated with increased incidence of ASSC, particularly when initiated before 2 years of age.
Blinatumomab infusion interruptions in pediatric patients rarely lead to readmission
Authors: Moody Jonathan , Barker Patricia J. , Sciasci Joseph , Pauley Jennifer L. , Bragg Allison , McMillan Cody , Triplett Brandon M. , Swanson Hope D. 
Published: July 2024

AbstractBlinatumomab is a bispecific T‐cell engager administered as a 28‐day continuous infusion. Infusions can be associated with interruptions requiring support from clinical staff, but the frequency of interventions with outpatient blinatumomab has not been characterized. This study is a single‐center, retrospective review of patients who received blinatumomab between December 3, 2014 and October 31, 2021 to determine frequency and type of interventions. Forty patients received blinatumomab for 69 cycles. Clinical staff intervention was required in 31 (45%) cycles, only six (8.7%) cycles needed readmission. Management of outpatient blinatumomab infusions requires education and training of clinical staff and caregivers to quickly troubleshoot interruptions.
Study on the anticancer effects of cold atmospheric plasma on deep subcutaneous tumor cells
Authors: Zhang Jishen , Xu Shengduo , Chen Jinkun , Yang Weiji , Wu Tong , Wang Zifeng , Guo Li , Liu Dingxin , Zhang Hao , Xu Yujing , Rong Mingzhe 
Published: July 2024

AbstractCold atmospheric plasma (CAP) therapy has been proven to effectively inhibit the growth of subcutaneous tumors. However, there is no research to explain how plasma‐induced anticancer effects can be transmitted to cancer cells through normal cells. Here, we monitored the effects of plasma‐irradiated HaCaT cells on nonirradiated A375 and HaCaT cells in terms of cell viability and intracellular reactive oxygen species levels in vitro. The experimental results indicated that plasma irradiation may induce plasma‐irradiated HaCaT cells to release soluble factors into the cell culture medium, thereby selectively killing nonirradiated A375 cells. The plasma‐generated short‐lived species play a crucial role in the release of soluble factors. Our research showed that plasma‐induced anticancer effects can be transmitted to deep nonirradiated cancer cells through plasma‐irradiated normal cells.
Cystic fibrosis year in review 2023
Authors: Swetland David V. , Savant Adrienne P. 
Published: July 2024

AbstractThis past year, there were many important advances for patients with cystic fibrosis (CF). Of the many publications related to CF in 2023, there was further evaluation of highly effective modulator therapy, new assessments and guidelines for clinical manifestations and therapies for CF, advances in newborn screening and diagnosis, and evaluation of outcomes for people with CF transmembrane conductance regulator‐related metabolic syndrome/CF screen positive, inconclusive diagnosis. The aim of this review article is not to provide a full assessment of the wide range of articles published in 2023, but to provide a brief review of publication that may lead to changes in clinical care.
Toward enhancing nonlinear observers for Lipschitz system: Exploiting the matrix multipliers‐based LMIs
Authors: Mohite Shivaraj , Alma Marouane , Zemouche Ali 
Published: July 2024

AbstractThis article is focused on the design of an LMI‐based observer for the class of disturbance‐affected nonlinear systems. Two novel LMI conditions are derived by deploying a more general form of the matrix multiplier compared to the one used in the literature. The first method is based on the use of the criterion, while the second one utilizes an ISS notion. Both LMIs are developed by employing the reformulated Lipschitz property, a well‐known LPV approach and the new variant of Young inequality. The key element of the proposed LMI conditions is the incorporation of the novel matrix multipliers which allow us to include some additional decision variables as compared to the methods proposed in the literature. These additional variables add extra degrees of freedom, thus enhancing the LMI feasibility. Furthermore, the effectiveness of the proposed methodologies is showcased through a numerical example.
Robust fault detection for switched systems with unstable modes: A bumpless transfer piecewise filtering approach
Authors: Liang Zhanhong , Gao Jinfeng , Yao Lina 
Published: July 2024

AbstractThis investigation addresses the bumpless transfer fault detection (FD) problem within switched systems that possess unstable modes. A switched piecewise FD filter is developed as a residual generator by segmenting each dwell time interval. The transition between different filters precipitates pronounced, abrupt jumps in the residual signal. Consequently, a novel definition of the residual bumpless transfer performance is introduced to constrain the magnitude of residual fluctuations during switching instances. Additionally, an alternating switching strategy—characterized by slow and fast mode‐dependent average dwell time and supported by the multiple discontinuous Lyapunov function method—is devised to assure the asymptotic stability and the weighted performance of the augmented system. Concurrently, sufficient conditions for the solvability of the bumpless transfer FD problem are articulated in the form of linear matrix inequalities. Lastly, a switched buck‐boost circuit and a numerical example are presented to substantiate the merits of the proposed bumpless transfer piecewise filtering method.
Assisted migration of coho salmon: Influences of passage and habitat availability on population dynamics
Authors: Benjamin Joseph R. , Dunham Jason B. , Scheidt Nicholas , Rothenbuecher Carla , Sipher Cory 
Published: July 2024

AbstractAssisted migration is a means of introducing a species into a previously unoccupied area. Although this idea is relatively new for many species, there are many extant examples involving fish that can be instructive. We studied a case of assisted migration where upstream access of migrating adult coho salmon Oncorhynchus kisutch over a naturally impassible barrier was established through construction of fish ladders. Although these passage structures have successfully allowed coho salmon to colonize upstream locations, managers had concerns regarding how efficiently these structures passed fish, as well as questions regarding access to specific upstream habitats, and passage barriers further upstream. To address these concerns, we developed a stage‐based population model to explore: (1) influences of passage over structures, (2) rearing habitats upstream of the structures, and (3) consequences of additional barriers to passage in the system. Model simulations suggest high fish passage at the ladders was associated with the highest smolt and adult abundance of coho salmon. The importance of passage was strongly influenced by juveniles rearing in a lake, where increased lake rearing at each passage scenario increased abundance of smolts and adults. Opening habitat further upstream was estimated to increase adult and smolt abundance up to 12%. Results of model simulations also helped to identify uncertainties that could be evaluated further (e.g., juvenile rearing in the lake). In general, our findings point to the importance of considering a full range of processes that can drive expected outcomes for assisted migration.
Soil health influenced more by conservation tillage and cropping practice than irrigation level in a sandy semiarid cotton system
Authors: Petermann Billi Jean , Lewis Katie , Acosta‐Martinez Veronica , Laza Haydee E. , Steffan Joshua J. , Slaughter Lindsey C. 
Published: July 2024

AbstractCropping systems in semiarid regions have frequently relied on continuous tillage and irrigation, but declining groundwater resources have prompted a greater focus on conservation practices to improve soil health and water storage. We compared soil health responses from cotton production systems in semiarid, coarse‐textured soils with different crop management strategies under high or low irrigation levels. Management systems included continuous cotton with conventional tillage (CCCT) compared to no‐till cotton with a rye cover crop (NTCR) and no‐till cotton with a wheat‐fallow rotation (NTCW), including high or low irrigation zones within each system. Samples were collected annually from two bulk soil depths (0–10 cm and 10–20 cm) and root‐associated soils 7 years after systems were established and continued for 2 years. We found that cropping system, but not irrigation level, altered soil microbial communities and other soil health indicators. Despite variation between study years and sampling zones, the conservation systems had greater soil microbial community size via ester‐linked fatty acid methyl ester (EL‐FAME or FAME) analysis, extracellular enzyme activities, and soil organic matter than the CCCT system. The NTCW system also had greater arbuscular mycorrhizal fungi FAME abundance. Our study suggests that no‐till and conservation strategies such as cover cropping and rotation can improve biological soil health indicators in these sandy semiarid soils even with limited irrigation.
Observation of a Reversible Order‐Order Transition in a Metal‐Organic Framework – Ionic Liquid Nanocomposite Phase‐Change Material
Authors: Nozari Vahid , Azar Ayda Nemati Vesali , Sajzew Roman , Castillo‐Blas Celia , Kono Ayano , Oschatz Martin , Keen David A. , Chater Philip A. , Robertson Georgina P. , Steele James M. A. , León‐Alcaide Luis , Knebel Alexander , Ashling Christopher W. , Bennett Thomas D. , Wondraczek Lothar 
Published: July 2024

AbstractMetal‐organic framework (MOF) composite materials containing ionic liquids (ILs) have been proposed for a range of potential applications, including gas separation, ion conduction, and hybrid glass formation. Here, an order transition in an IL@MOF composite is discovered using CuBTC (copper benzene‐1,3,5‐tricarboxylate) and [EMIM][TFSI] (1‐ethyl‐3‐methylimidazolium bis(trifluoromethanesulfonyl)imide). This transition – absent for the bare MOF or IL – provides an extended super‐cooling range and latent heat at a capacity similar to that of soft paraffins, in the temperature range of ≈220 °C. Structural analysis and in situ monitoring indicate an electrostatic interaction between the IL molecules and the Cu paddle‐wheels, leading to a decrease in pore symmetry at low temperature. These interactions are reversibly released above the transition temperature, which reflects in a volume expansion of the MOF‐IL composite.
Catalytic Potential of Supported Superatoms
Authors: Kilic Mehmet Emin , Jena Puru 
Published: July 2024

AbstractThe importance of catalysts in industrial products is a driving factor in the search of efficient and cost‐effective catalysts, creating considerable interest in the past decade in single‐atom catalysis. One of the first requirements of a good catalyst is that it should bind to the molecules with energies intermediate between physisorption and chemisorption while simultaneously activating them. Herein, it is shown that superatoms, which are atomic clusters with fixed size and composition, can meet this challenge even better than the atoms whose chemistry they mimic. The reactions of molecules such as H2, O2, N2, CO, NO, and CO2 with an atom (Li) and its corresponding superatom (Li3O) are confirmed through study. As these clusters need to be supported on a substrate for practical applications, the study focuses on the reaction of CO2 with Li and Li3O supported on graphene, Au(111), and Cu(111) substrates. Using density functional theory, it is shown that the Li3O superatom can activate CO2 far greater than the Li atom – stretching the CO bond from 1.16 Å to as large as 1.30 Å and bending the O─C─O bond angle from 180° to as low as 120°. Equally interesting, the results are not very sensitive to the substrate.