bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propuesta metodológica para la determinación de las épocas climáticas con aplicación a los estudios de producción y reproducción bovina en el trópico
Authors: Rivera Magda , Ramírez Julio , Moncada Hemerson , Trujillo Luis E. 
Published: August 2024

Resumen El efecto del clima sobre el comportamiento productivo y reproductivo en plantas y animales se evidencia a través de la producción de forraje de la pradera, la ganancia de peso de los animales o la distribución de sus partos a lo largo del año. Con el fin de determinar el período climático más favorable para el desempeño productivo del ganado es prioritario caracterizar y clasificar el clima con base en los factores que más lo afectan. La caracterización se logra a través de la elaboración del balance hídrico o contabilidad del agua en el suelo, metodología que tiene en cuenta la precipitación pluvial, la evapotranspiración potencial y real y el movimiento del agua a través del perfil del suelo. El efecto del clima sobre los parámetros productivos y reproductivos se evalúa tomando en cuenta las características climáticas en los cuatro o cinco meses previos a la fecha de corte del análisis y con base en este criterio se crea la base de datos. El análisis estadístico emplea los procedimientos del Modelo Lineal General (GLM) del Statistical Analysis System (SAS) y los coeficientes de correlación y regresión. La metodología climática y estadística propuesta es aplicable a los sistemas de producción animal como una herramienta para el análisis e interpretación de los eventos productivos y reproductivos en los bovinos. Palabras clave: bovinos, clima, evapotranspiración, hídrico, producción, reproducción. Summary The climatic effect on productive and reproductive performance in plants and animals is reflected on the forage production of a prairie, the weight gain of the animals or the distribution of their calvings along the year. In order to determinate the most favorable climatic period to the productive behavior of cattle, it's necessary to characterize and classify the climate depending on the factors that most affect it. The characterization is achieved through the elaboration of the hydric balance, that is the account of soil water, a methodology that takes in account the rainfall, the potential and real evapotranspiration and the movement of water through the soil outline. The climatic effect on productive and reproductive characteristics is evaluated considering the climate during four or five months prior to the date of analysis. The database is created based on this criteria. The statistical analysis uses the General Lineal Model procedures of Statistical Analysis System, as also correlation and regression coefficients. The climatic and statistic methodology is applicable to animal production systems as a tool for the analysis and explanation of productive and reproductive events in bovine cattle. Key words: bovine, climate, evapotranspiration, hydric, production, reproduction.
Injertos óseos - Nueva alternativa. Fase I. Extracción de proteínas morfogenéticas parcialmente purificadas de hueso bovino
Authors: Rivera Posada Jairo , Riaño Carlos H. , Monsalve Paula A. , Osorio Adriana 
Published: August 2024

Resumen El propósito de esta investigación fue extraer a partir de hueso bovino y mediante procedimientos químicos, Proteínas Morfogenéticas Óseas (PMO), parcialmente purificadas que posean características osteoinductivas, de alta calidad, baja antigenicidad y alta biodisponibilidad, para ser utilizadas como material de injerto óseo. El procedimiento se llevó a cabo mediante cinco etapas básicas así: Molienda del material; extracción de lípidos; desmineralización; extracción del colágeno; obtención de la fracción proteica. Para verificar la efectividad de la desmineralización y demás procesos, se procedió como sigue: Método de Soxhlet; espectrometría de Absorción Atómica de Calcio; tinción de picrofucsina de Van Gieson; electroforesis en Gel de Poliacrilamida-Dodecil Sulfato Sódico. Se obtuvo un material con proteínas de peso molecular aparente en el rango de 18 a 120 KDa, principalmente en las bandas correspondientes a 18, 24, 34, 68, 72, 90, 120 KDa, mediante electroforesis en Gel de Poliacrilamida Dodecil Sulfato Sódico (SDS-PAGE) el cual fue liofilizado y almacenado en ampollas de vidrio selladas al vacío para su conservación y uso posterior. Palabras clave: biomateriales, factores de crecimiento, osteoregeneración. Summary The purpose of this research was to extract from bovine bone and through physical and chemical procedures, Bone Morphogenetic Proteins (BMPs) partially purified, with high-quality osteoinduction characteristics, low antigenicity and high bioavailability. These proteins will be utilized as bone graft material. The procedure was performed through five basic steps: Milling the bovine bone, lipid extraction, demineralization, collagen extraction, obtention of the protein fraction. These steps were verified respectively through the following analysis: The Soxhlet method, Atomic Absorption for Calcium determination, Van Gieson Pycrofucsin Stain., Polyacrilamide Gel-Electrophoresis-SodiumDuodecyl-Sulphate (PAGE-SDS). BMPs partially purified were obtained through Polyacrilamide Gel-Electrophoresis-Sodium-Duodecyl-Sulphate (PAGE-SDS., with molecular weights between 18 - 120 Kda, mainly bands at 18, 24, 34, 68, 72, 90 and 120 KDa, This material was lyophilized, packed and stored in a vacuum sealed narrow-neck bottle for posterior use. Key words: biomaterials, growth factors, osteoregeneration.
Manejo médico y quirúrgico del síndrome de las vías aéreas superiores del braquicéfalo. Reporte de un caso
Authors: Orozco P. Sonia C. , Gómez G. Leonardo F. 
Published: August 2024

Resumen En este artículo se describe el manejo médico y quirúrgico de un paciente de raza Pug, macho de 3 años, que presentaba frecuentemente disneas inspiratorias, las cuales eran debidas a las anormalidades anatómicas que hacen parte del síndrome braquicefálico. Palabras clave: canino, colapso, disnea, narinas, paladar blando. Summary This article is a case report, that describes the surgical and medical treatment of a 3 year old male Pug, that had severe dyspneas after a convulsive crisis due to anatomical abnormalities that make part of the brachycephalic airway syndrome. Key words: canine, collapse, dyspnea, nares, soft palate.
Interrupción temporal de la lactancia (ITA) y presentación de estro en vacas cebú posparto
Authors: Mahecha Liliana , Henao Damary , Cardona Mariluz , Olivera A. Martha 
Published: August 2024

Resumen Este estudio se llevó a cabo en vacas cebú amamantando bajo condiciones de trópico bajo, pastoreando brachiaria en condiciones semintensivas y con días abiertos mayores a 150. Con el fin de determinar si la interrupción temporal del amamantamiento (ITA) disminuye los días abiertos, se escogieron 34 vacas con 80 a 120 días abiertos y ciclando. Se dividieron en dos grupos al azar, uno de 19, a las que se les retiró el ternero durante 72 horas y otro de 15 controles. Todas las vacas pastorearon juntas. La semana previa al experimento, durante la restricción y hasta dos semanas después, se realizaron ecografías y detección de calor. Las variables consideradas fueron la tasa de crecimiento folicular durante el ITA y la tasa de crecimiento del folículo dominante, así como la presentación de calor. El 94.7% de las vacas con ITA presentaron calor durante el tratamiento, o en la semana siguiente, aparentemente ejercieron un efecto social sobre las compañeras sin tratamiento, ya que en estas se presentaron 46.7% de calores simultáneamente. El tratamiento afectó positivamente el diámetro folicular de las vacas tratadas. Palabras clave: amamantamiento, divergencia folicular, ovulación. Summary This study was carried out in suckled Zebu Brahman under low altitude, tropical semi-intensive conditions, characterized by long opened-day periods (>150 days). This low reproductive efficiency is said to be due to suckling that takes place for about 8 months postpartum. 34 cows grazing Brachiaria humidicola were studied, beginning non earlier than 80 days postpartum and all of them presenting follicular dynamics as detected by ultrasonography. The cows were allocated to one of two groups: restricted (19) or non-restricted (15). The restriction consisted of a one- time isolation of calves for 72 hours. During the restriction and for two more days daily ecographic examination was performed. There after the cows were examined ecographically twice a week for two more weeks.. Observation for clinical signs of heat was done twice a day for one-hour period during the restriction and 10 days after. The variables considered were dominant follicular growth at divergency during restriction, and estrus presentation. Effect of restriction on estrus was analyzed with a logistic regression model, and the follicular growth with a general linear model p< 0.05. No significant difference was found in the growth rate of divergent follicles between control and treatment cows (0.87 mm/day vs. 0.93 mm/day). All but one (94.7%) of restricted cows presented heat during the treatment, but interestingly, seven out of fifteen (46.6%) of the control did too, and this was significantly different (p< 0.05). The practical end result is as good as expected but it remains to be determinined why a high unexpected number of control cows did present estrus too. Key words: divergency, ovulation, restriction.
Injertos óseos - Nueva alternativa. Fase II. Evaluación de las propiedades osteoinductoras de las proteínas morfogenéticas óseas parcialmente purificadas, implantadas en tejido subcutáneo de un modelo experimental lapino
Authors: Rivera Posada Jairo A. , Riaño Carlos H. , Monsalve Paula A. , Restrepo Luis F. , Aranzazu Diego , Giraldo Gloria E. , Alzate G. Gildardo J. , Jaramillo Carlos D. , De León María V. , Hernández Elizabeth 
Published: August 2024

Resumen El propósito de esta investigación fue evaluar las características osteoinductivas de las Proteínas Morfogenéticas Óseas (PMO) parcialmente purificadas de hueso bovino, mediante implantación subcutánea en un modelo experimental lapino, para ser utilizadas posteriormente como material de injerto óseo en combinación con un material osteoconductor. El procedimiento se llevó a cabo mediante la implantación subcutánea de ocho mg de PMO en la región dorsal a nivel de la escápula, en 21 conejos de Raza Blanca de Nueva Zelanda, los cuales se distribuyeron en 6 grupos de evaluación así: tres, seis, nueve, doce, veintiún días y ocho semanas de evolución, transcurridos estos tiempos se retiraron los implantes para ser evaluados histopatológicamente por microscopía óptica convencional y así caracterizar los diferentes elementos tisulares. Los resultados obtenidos se analizaron utilizando el método factorial de correspondencias múltiples con clasificación jerárquica, el cual mostró que las PMO estimulan la inducción de hueso en sitios ectópicos, teniendo actividad osteogénica ya que se observó presencia de trabéculas óseas en todas las muestras tomadas a partir del sexto día postimplante. Palabras clave: factores de crecimiento, implantes, osteoinducción. Summary The purpose of this research was to evaluate the osteoinductive characteristics of partially purified bone morphogenetic proteins from bovine bone, through subcutaneous implantation in an experimental lapin model, to be used later as bone graft material mixed with an osteoconductive material. The procedure was carried out through subcutaneous implantation of 8 mg of BMP in the dorsal region at scapula level, in 21 New Zealand rabbits. They were distributed into 6 evaluation groups at 3,6,9,12,21 days and 8 weeks of evolution, when the implants were removed and sent to histopatologic evaluation through convencional optic microscopy to characterize different tissue elements, that included bone neoformation. The results were analyzed with multiple correspondence factorial method with jerarquic classification, and showed that BMP stimulate bone induction in ectopic sites, having osteogenic activity, because osseous trabeculae were noticed in all samples taken since day 6 post implantation. Key words: grafts, growth factors, osteoinduction.
Naturaleza y control de la quimiorresistencia en ectoparásitos
Authors: Errecalde Carlos , Prieto Guillermo , Lüders Carlos , García Hugo 
Published: August 2024

Resumen En la actualidad, con trascendencia e intensidad variable, la resistencia se extiende a todos los ectoparásitos combatidos y comprende a todos los compuestos químicos disponibles condicionando seriamente la efectividad. Es un fenómeno preadaptativo, heredable, originado de una mutación, por lo tanto su concepción es genética. La habilidad de supervivencia se difunde en una población determinada pues el principio activo excluye los individuos susceptibles, seleccionando en los sobrevivientes genes heredados por generaciones sucesivas. La resistencia se manifiesta por diferentes mecanismos, entre los cuales se destaca la penetración reducida, detoxificación incrementada o menor sensibilidad por el sitio blanco, no obstante ciertas características genéticas y biológicas del parásito que favorecen la expresión, son significativos los factores operacionales, inherentes al manejo del principio activo que favorecen la presión de selección, tales como el empleo de dosis elevadas, administraciones repetidas o de acción residual intrínseca, en consecuencia la prevención de la resistencia no sólo debe contemplar el monitoreo permanente de la susceptibilidad del parásito que se pretende controlar sino también es necesario adoptar medidas tendientes a reducir la presión de selección. Palabras clave: control, ectoparásitos, insecticidas, resistencia. Summary At the present time, with variable importance and intensity, the resistance extends to all the fought ectoparasites and includes all chemical compounds available, conditioning seriously its effectiveness. It is a preadaptive, inheritable phenomenon, originated from a mutation, with a genetic conception. The survival ability spreads in a certain population because the active principle excludes the susceptible individuals, selecting in the surviving ones, genes inherited by successive generations. The resistance is pronounced by different mechanism; between them, the reduced penetration stands out, increased detoxification or smaller sensitivity by the target site, despite certain genetic and biological characteristics of the parasite that favors the expression, the operational and inherent factors are significant to the handling of the active principle which favors the selection pressure, such as the use of elevated doses, repeated administrations or of intrinsic residual action, consequently the prevention of the resistance must contemplate permanent monitoring of the susceptibility of the parasite to be controlled, and, if necessary, measuring to reduce the selection pressure. Key words: control, ectoparasites, insecticide, resistance.
Ehrlichiosis canina
Authors: Benavides Javier A. , Ramírez Gines F. 
Published: August 2024

Resumen En este artículo se reporta un caso de Ehrlichiosis canina, diagnosticado mediante la detección de antígenos de Dirofilaria immitis, Borrelia burgdorferi y anticuerpos de Ehrlichia canis en un canino macho labrador dorado, con un peso de 36 kg y 6 años de edad. Se mencionan los procedimientos realizados para el diagnóstico, incluida la valoración clínica, exámenes de laboratorio y seguimiento terapéutico. Palabras clave: canino, ehrlichia, ricketsia. Summary In this article a case of canine Ehrlichiosis is reported, diagnosed by means of antigens detection of Dirofilaria immitis, Borrelia burgdorferi and Ehrlichia canis antibodies, in a canine male, golden labrador, with a 36 kg weight and 6 years old. The procedures made for the diagnosis are mentioned, including the clinical examitatión, laboratory tests and therapeutic. Key words: canine, ehrlichia, ricketsia.
Developing Psychomotor Skills and Social Attributes Through Integrated Psychomotor and Unified Football Training: A Special Olympics Intervention Study
Authors: Stefanica Valentina , Mercea Ionut-Traian , Ursu Vasile Emil , Gorban Corneliu Florin , Dragos Cristian , Mihaiu Costinel , Gogean Groza Gina 
Published: August 2024

This research investigates the combined effects of a tailored psychomotor development training and Unified Sports football program on psychomotor abilities and social attributes among Special Olympics athletes with intellectual disabilities aged 16-18 in Romania. The study aims to assess the impact of the integrated psychomotor and Unified Football training program on the psychomotor abilities of youth Special Olympics athletes with intellectual disabilities, evaluate the influence of the intervention on their social attributes, and examine the relationship between psychomotor abilities and social attributes among participants. The findings indicate significant improvements in psychomotor skills, including Static Balance (t = -8.042, p < 0.001), Identified Stimuli (t = -7.000, p < 0.001), and Friendship Activity Scale scores (t = -8.990, p < 0.001), reflecting substantial enhancements from initial to final testing. Moreover, Pulse Processing Speed showed a significant improvement (t = -2.299, p = 0.042). However, while Dynamic Balance exhibited a negative t-value (t = -2.021), the p-value of 0.068 indicates a marginally significant improvement. These results suggest notable advancements in psychomotor skills and social interaction among Special Olympics athletes, underscoring the effectiveness of the integrated training program. The implications of these findings extend to coaches and practitioners involved in designing tailored training programs aimed at enhancing psychomotor and social skills among athletes with intellectual disabilities.
Optimization of Hand Sanitizer Formulation with the Addition of Lemongrass (Cymbopogon citratus (DC.) Stapf) Essential Oil and a Combination of Essential Oils of Local Indonesian Empon-empon
Authors: Ariani Sri Retno Dwi , Van Hayus Elfi Susanti , Mulyani Sri , Utomo Suryadi Budi , Wathon Muhammad Hizbul , Bilhaq Natiq , Pramesti Anastasia Diaz 
Published: August 2024

ABSTRACT: This research aimed to optimize the formulation of hand sanitizer by incorporating lemongrass (Cymbopogon citratus (DC.) Stapf) essential oil in combination with essential oils of local Indonesian empon-empon: temu giring (Curcuma heyneana Val. & van Zipj.), temu kunci (Kaempferia pandurata Roxb.), and temu mangga (Curcuma amada Roxb.). The optimization of hand sanitizer formulation was done based on several tests. The first was organoleptic test by observing the aroma, color, and form; the second was pH, homogeneity, and spreadability testing, and; the third was testing the antibacterial activities of each hand sanitizer formula against Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25922. The research stages encompassed isolation of essential oils, formulation and evaluation of hand sanitizers, antibacterial activities test, optimization of hand sanitizer formulation, and identification of the chemical compounds in the hand sanitizer with the optimum formulation using the GC-MS method. The study resulted in the hand sanitizer with the optimum formulation containing the essential oils of lemongrass (3%) and temu giring (3%), demonstrating very strong antibacterial bioactivities against both E. coli ATCC 25922 and S. aureus ATCC 25923, with inhibition zones measuring 29.70 mm and 60.08 mm, respectively. This hand sanitizer had a distinctive aroma, combining the scents of lemongrass and temu giring. It was in liquid form, clear, and homogeneous, with pH and spreadibility values of 5.4 and 6.4 cm, respectively. The quality complied with SNI quality standards No. 06-2588. The primary active ingredients were E-citral (22.10%), Z-citral (17.88%), and 1,8-cineol (17.63%).

Keywords: Essential oil, hand sanitizer, lemongrass, temu giring, temu kunci, temu mangga.
The Potential of Paku Gajah (Angiopteris evecta) as Antitumor Through In Vitro and In Silico Studies
Authors: Rasyid Herlina , Asmirah Asmirah , Firdausiah Syadza , Arief Ihsanul , Soekamto Nunuk Hariani 
Published: August 2024

ABSTRACT. Paku gajah (Angiopteris evecta) is one of the largest ferns which has been used empirically by the Dayak tribe of Kalimantan, Indonesia as a traditional medicine to treat various diseases, one of which is tumors. This research aims to determine the potential utilization of A. evecta stem extract as an antitumor by secondary metabolites analysis, toxicity and antitumor assay. The methods used in this study were gradual maceration using three solvents (n-hexane, ethyl acetate, and methanol), phytochemical screening, toxicity test, antitumor activity assay with the Alamarblue method, Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, and molecular docking analysis. This study indicated that A. evecta stem extract contained secondary metabolites such as flavonoids, saponins, tannins, and steroids. The ethyl acetate and methanol were found as toxic extracts with LC50 130.67 and 314.31 µg/mL, respectively. In line with the toxicity, the antitumor activity of the ethyl acetate extract was the highest with an IC50 of 240.94 µg/mL and phytochemical analysis revealed the presence of violanthin and angiopteroside in the extract. Molecular docking showed that the binding energy and inhibition constants of violanthin and angiopteroside against receptors were higher than standard ligand F82. The interaction between violanthin and the receptor results five hydrogen-bond (H-bond) with Lys920, Cys919, Asp1046, and Leu840, while the angiopteroside produces four H-bonds with Leu836, Leu834, and Arg831.

Keywords: Antitumor, molecular docking, paku gajah (Angiopteris evecta).
Application of FTIR Spectroscopy and Chemometric to Differentiate Azadirachta excelsa (Jack.) Jacobs Leaves Extracts Based on Solvent Polarity and Assessment of Antibacterial Activity
Authors: Adfa Morina , Erliana Dina , Wiradimafan Khafit , Triawan Deni Agus , Yudha S. Salprima , Avidlyandi Avidlyandi , Rafi Mohamad 
Published: August 2024

ABSTRACT. Azadirachta excelsa is a plant belonging to the same genus as the Indian neem (Azadirachta indica) which is expected to have similar biological activities. However, its active components and pharmacological effects are limited. The composition and quantity of these metabolites in A. excelsa may differ due to different polarities of extracting solvents, so selecting an effective extractive solvent with a high level of biological activity is important. Therefore, in this study, we examined differences in the metabolite finger printing using FTIR-based metabolomics, as well as evaluated their antibacterial activity against Staphylococcus aureus (ATCC 6538) and Escherichia coli (ATCC 8739). A. excelsa was extracted using chloroform, ethyl acetate, ethanol p.a., 70% and 50% ethanol. Extracts obtained were analyzed using FTIR and the inhibition zones were then determined. The results showed that principal component analysis (PCA) could distinguish each sample based on the extraction solvent. In this study, we found 50% and 70% ethanol extracts had similar metabolite compositions and concentrations based on their respective FTIR spectrum. The inhibition zone of A. excelsa extracts ranged from 12.37-17.20 mm and 13.88-15.89 mm against S. aureus and E.coli, respectively. The chloroform extract was more effective against both bacteria. Duncan's further test showed that chloroform extract reduced E. coli similarly to ethyl acetate but not S. aureus. While 50%, 70%, and ethanol p.a. extracts inhibited S. aureus and E. coli equally. Based on these results, the polarity of the extracting solvent had an important influence on the metabolite profile and antibacterial activity of A. excelsa.

Keywords: Azadirachta excelsa, antibacterial activity, metabolite fingerprinting, FTIR.