bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ethanol facilitates hepatitis C virus replication via up-regulation of GW182 and heat shock protein 90 in human hepatoma cells
Authors: Bukong Terence N. , Hou Wei , Kodys Karen , Szabo Gyongyi 
Published: August 2012

Abstract
Alcohol use and hepatitis C virus (HCV) infection synergize to cause liver damage, and microRNA-122 (miR-122) appears to play a key role in this process. Argonaute 2 (Ago2), a key component of the RNA-induced silencing complex (RISC), has been shown to be important in modulating miR-122 function during HCV infection. However, GW182, a critical component of processing bodies (GW bodies) that is recruited by Ago2 to target messenger RNA (mRNA), has not been assessed in HCV infection. To characterize the role of GW182 in the pathogenesis of HCV infection, we determined its transcription and protein expression in an HCV J6/JFH1 culture system. Transcript and protein levels of GW182 as well as HCV RNA and protein expression increased with alcohol exposure. Specific silencing of mRNA expression by small interfering RNA against GW182 significantly decreased HCV RNA and protein expression. Overexpression of GW182 significantly increased HCV RNA and protein expression in HCV J6/JFH1 infected Huh7.5 cells. Furthermore, GW182 colocalized and coimmunoprecipitated with heat shock protein 90 (HSP90), which increased upon alcohol exposure with and without HCV infection and enhanced HCV gene expression. The use of an HSP90 inhibitor or knockdown of HSP90 decreased GW182 and miR-122 expression and significantly reduced HCV replication. Conclusion: Overall, our results suggest that GW182 protein that is linked to miR-122 biogenesis and HSP90, which has been shown to stabilize the RISC, are novel host proteins that regulate HCV infection during alcohol abuse. (Hepatology 2013)
Distribution of Cyprideis torosa (Ostracoda) in Quaternary Athalassic Sediments in Germany and its Application for Palaeoecological Reconstructions
Authors: Pint Anna , Frenzel Peter , Fuhrmann Roland , Scharf Burkhard , Wennrich Volker 
Published: August 2012

AbstractCyprideis torosa (Jones, 1850) is a very common brackish water ostracod of the German coasts, but, despite empty valves are found occasionally in surface sediments of some modern inland waters, C. torosa could not be found living in modern athalassic waters of Germany so far. During interglacial periods, including the Holocene, however, fossils of this species are quite common in Central Germany, at a distance of more than 300 km away from the coasts of the Baltic and North Seas. All 31 Quaternary localities with C. torosa known so far from Germany are documented. C. torosa is an indicator for brackish waters and widely used as index‐fossil in palaeosalinity reconstructions relying on water chemistry bound morphological changes (nodes, sieve‐pores). The comparisons imply a general underestimation of palaeosalinity in oligo‐ to mesohaline athalassic waters if using nodes and sieve‐pores of C. torosa as proxy. A water chemistry (ionic composition) driven morphological response is assumed instead one by salinity only. Palaeosalinity estimations for athalassic waters, relying on morphological variability alone, should therefore be used with caution. Palaeosalinity trends, however, can be detected. Distinguishing thalassic and athalassic sediments with C. torosa is possible by using the associated ostracod fauna as a discriminator. Regarding the ecology and distribution of C. torosa, permanent, brackish, and shallow water bodies under relatively warm conditions are required for its settlement. The source of the salt are brines originating from Zechsteinian or Triassic underground evaporites. Warm and relatively dry climates could enhance the process for such water bodies of becoming salty, a situation present in Holocene Central Germany. The occurrence of C. torosa can therefore be used for palaeoclimatological studies. The most probable migration path of this ostracod species to athalassic waters is by avian transport. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Late Glacial and Holocene Ostracoda of the Gulf of Gdańsk, the Baltic Sea, Poland
Authors: Krzyminska Jarmila , Namiotko Tadeusz 
Published: August 2012

AbstractTo trace environmental changes in water hydrology and salinity in the Late Glacial to Holocene of the Gulf of Gdańsk, a south‐eastern bay of the Baltic Sea within the maritime zone of Poland, the distribution of ostracod valves was studied in 20 sediment cores collected from both the shallow‐ and deep‐water zones (depth 10.9–67.5 m). The studied sediment sequences yielded ca. 3000 valves of 21 ostracod species, of which only five are known to live today in the Gulf, which has a present maximum depth of 118 m and water salinity up to 7–8‰. The majority of the studied sediment layers that contained ostracod valves corresponded to the period of the Late Glacial to Mid‐Holocene and was dominated by non‐marine species, of which the most common were Candona neglecta (present in 17 cores), Cytherissa lacustris (15 cores) and Candona candida (14 cores). By clustering classification five major ostracod assemblage types were recognised in the studied cores. Initial assemblage types dominated mostly by inhabitants of the profundal/sub‐littoral zones of modern oligo‐mesotrophic lakes (C. lacustris and C. neglecta) in some sediment sequences were replaced in stratigraphical order by the assemblages dominated by brackish‐water species (Cyprideis torosa or Cytheromorpha fuscata). The structure and species composition of the distinguished ostracod assemblage types as well as their successional transitions indicate that the studied sediments were deposited initially in the Late Glacial in freshwater lacustrine conditions, and subsequently, during the Holocene marine transgression, covered by marine sands. The present results confirm and consolidate inferences based on previously published data on ostracods from the western part of the Gulf of Gdańsk as well as on other biotic (molluscs, diatoms) and abiotic (seismoacoustic) indices from this area (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Correlation of temperature rise and optical coherence tomography characteristics in patient retinal photocoagulation
Authors: Koinzer Stefan , Schlott Kerstin , Portz Lea , Ptaszynski Lars , Baade Alexander , Bever Marco , Saeger Mark , Caliebe Amke , Denner Renè , Birngruber Reginald , Brinkmann Ralf , Roider Johann 
Published: August 2012

AbstractWe conducted a study to correlate the retinal temperature rise during photocoagulation to the afterward detected tissue effect in optical coherence tomography (OCT). 504 photocoagulation lesions were examined in 20 patients. The retinal temperature increase was determined in real‐time during treatment based on thermoelastic tissue expansion which was probed by repetitively applied ns laser pulses. The tissue effect was examined on fundus images and OCT images of individualized lesions. We discerned seven characteristic morphological OCT lesion classes. Their validity was confirmed by increasing visibility and diameters. Mean peak temperatures at the end of irradiation ranged from approx. 60 °C to beyond 100 °C, depending on burn intensity. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The Course of Case Linkage Never Did Run Smooth: A New Investigation to Tackle the Behavioural Changes in Serial Car Theft
Authors: Davies Kari , Tonkin Matthew , Bull Ray , Bond John W. 
Published: August 2012

AbstractThis study aimed to investigate the case linkage principles, behavioural consistency and distinctiveness, with a sample of serial car thieves. Target selection, acquisition, and disposal behaviours, as well as geographical and temporal behaviours, were examined. The effects of temporal proximity and offender expertise were also investigated as moderating factors of behavioural consistency. As in previous case linkage research, geographical and some target selection behaviours were able to predict whether crime pairs are linked or unlinked at a statistically significant level. Crucially, it was also found that temporal behaviours demonstrate a significant capability to predict linkage status, a variable which has never before been applied to the prediction of linkage in serial car theft. Furthermore, it was demonstrated that changing the operationalisation of the behavioural domains can affect the results obtained. No support was found for the moderation of behavioural consistency on the basis of temporal proximity or expertise. Overall, the results support previous case linkage studies, furthering their practical applicability within the criminal justice system. Copyright © 2012 John Wiley & Sons, Ltd.
When does centrality matter? Scientific productivity and the moderating role of research specialization and cross‐community ties
Authors: Rotolo Daniele , Messeni Petruzzelli Antonio 
Published: August 2012

SummaryThe present study addresses the ongoing debate concerning academic scientific productivity. Specifically, given the increasing number of collaborations in academia and the crucial role networks play in knowledge creation, we investigate the extent to which building social capital within the academic community represents a valuable resource for a scientist's knowledge‐creation process. We measure the social capital in terms of structural position within the academic collaborative network. Furthermore, we analyse the extent to which an academic scientist's research specialization and ties that cross‐community boundaries act as moderators of the aforementioned relationship. Empirical results derived from an analysis of an Italian academic community from 2001 to 2008 suggest academic scientists that build social capital by occupying central positions in the community outperform their more isolated colleagues. However, scientific productivity declines beyond a certain threshold value of centrality, hence revealing the existence of an inverted U‐shaped relationship. This relationship is negatively moderated by the extent to which an academic focuses research activities in few scientific knowledge domains, whereas it is positively moderated by the number of cross‐community ties established. Copyright © 2012 John Wiley & Sons, Ltd.
Rapid determination of alkylphenols in aqueous samples by in situ acetylation and microwave‐assisted headspace solid‐phase microextraction coupled with gas chromatography–mass spectrometry
Authors: Wu Yu‐Pei , Wang Yu‐Chen , Ding Wang‐Hsien 
Published: August 2012

A rapid and solvent‐free procedure for the determination of 4‐tert‐octylphenol and 4‐nonylphenol isomers in aqueous samples is described. The method involves in‐situ acetylation and microwave‐assisted headspace solid‐phase microextraction prior to their determination using gas chromatography–ion trap mass spectrometry operated in the selected ion storage mode. The dual experimental protocols to evaluate the effects of various derivatization and extraction parameters were investigated and the conditions optimized. Under optimized conditions, 300 μL of acetic anhydride mixed with 1 g of potassium hydrogencarbonate and 2 g of sodium chloride in a 20 mL aqueous sample were efficiently extracted by a 65 μm polydimethylsiloxane‐divinylbenzene fiber that was located in the headspace when the system was microwave irradiated at 80 W for 5 min. The limits of quantitation were 5 and 50 ng/L for 4‐tert‐octylphenol and 4‐nonylphenol isomers, respectively. The precision for these analytes, as indicated by relative standard deviations, were less than 8% for both intra‐ and inter‐day analysis. Accuracy, expressed as the mean extraction recovery, was between 74 to 88%. A standard addition method was used to quantitate 4‐tert‐octylphenol and 4‐nonylphenol isomers, and the concentrations ranged from 120 to 930 ng/L in various environmental water samples.
Angular effect of optical fiber movement on endoscopic laser prostatectomy
Authors: Rajabhandharaks Danop , Kang Hyun Wook , Oh Junghwan 
Published: August 2012

AbstractBackground and ObjectiveThe optimal fiber manipulation during laser prostatectomy has been highlighted as a critical element to achieve desirable clinical outcomes. However, scientific understanding of the physical interplay between fiber movement and ablative tissue response is still lacking. The objective of this study was to quantitatively investigate the effect of angular movement of an optical fiber on tissue ablation performance.Study Design/Materials and MethodsPorcine kidney was employed as a tissue model in vitro. A 180 W 532 nm surgical laser with 750 µm side‐firing fibers was utilized to mimic clinical laser prostatectomy. The effect of fiber manipulation parameters on the tissue such as irradiance, number of overlapping pulses (OP), and beam path length (BPL) was assessed at various fiber sweeping (rotational) angles ranging from 0° to 120°. Morphological properties of the post‐irradiated tissue were also evaluated in light of ablation depth, coagulative necrosis, and volumetric ablation density (VAD).ResultsAs sweeping angle (SA) increased, both laser irradiance and number of OP decreased but BPL increased. Ablation depth was maximized (5.4 ± 1.0 mm) at SAs less than 30° but decreased at higher SAs. The SAs of 15° and 30° demonstrated the minimal thickness of denaturized tissue (0.74 ± 0.14 mm) and VAD (total laser energy/ablation volume (AV) ≈ 4.6 ± 0.46 J/mm3). Decreasing depth and increasing tissue coagulation associated with increasing SA resulted from substantial reduction in both beam irradiance and number of OP, eventually impeding ablation process. Excessive tissue denaturation also occurred when no rotational motion was applied to the fiber possibly due to plume shielding.ConclusionInefficient tissue ablation could lead to adverse post‐operative complications due to unwanted thermal injury to peripheral tissue. A SA of 30° was found to be desirable for effective tissue ablation, and further clinical investigations will validate the current findings. Lasers Surg. Med. 44: 653–663, 2012. © 2012 Wiley Periodicals, Inc.
Photodynamic therapy with systemic meta‐tetrahydroxyphenylchlorin in the treatment of anal intraepithelial neoplasia, grade 3
Authors: van der Snoek Eric M. , den Hollander Jan C. , Aans Jan B. , Sterenborg Henricus J.C.M. , van der Ende Marchina E. , Robinson Dominic J. 
Published: August 2012

AbstractBackground and ObjectiveAnal cancer and preneoplastic anal lesions (anal intraepithelial neoplasia, AIN) rising especially in men having sex with men (MSM). There are no widely accepted treatment standards for AIN. Photodynamic therapy (PDT) using the systemic sensitizer meta‐tetrahydroxyphenylchlorin (mTHPC) has the potential to treat the anal area even when the exact borders of the preneoplastic anal lesion cannot easily be visualized.Study Design/Materials and MethodsIn this prospective intervention study, 15 HIV‐positive MSM with AIN 3 were treated in 25 PDT‐sessions using mTHPC intravenously administered at drug doses of 0.075–0.15 mg ml−1 and illumination at 48 hours. The illumination was performed using a custom made applicator using either red light (652 nm) to a measured intended fluence of 10 and 20 J cm−2 and green light (532 nm) to a measured intended fluence of 105, 210, and 340 J cm−2. Red and green illuminations were performed at a (green) equivalent fluence rate of 105 mW cm−2.ResultsInitial complete response was seen in 7/25 (28%) of treatments and another 4/25 (16%) initial partial responses. After an average 8 months, recurrences were detected in 7/11 (64%) of sessions that initially showed response. A total 4/25 (16%) showed persistent complete response 6–15 months after green light illumination. Red light illuminations caused more significant side effects combined with no persistent complete response. Reported side effects were intense pain, bloody and purulent rectal discharge, and anal stricture formation, in one patient.ConclusionThe results show that the use of systemic mTHPC is partially effective for the treatment of AIN 3. Lasers Surg. Med. 44: 637–644, 2012. © 2012 Wiley Periodicals, Inc.
Evaluation of combined fractional radiofrequency and fractional laser treatment for acne scars in Asians
Authors: Yeung Chi K. , Chan Nicola P.Y. , Shek Samantha Y.N. , Chan Henry H.L. 
Published: August 2012

AbstractBackground and ObjectivesFractionated radiofrequency (RF) induces deep dermal heating and leaves the epidermis less affected. We studied the efficacy and safety of bipolar RF and fractional diode laser followed by fractional RF in Asians with acne scars.Materials and MethodsTwenty‐four patients (skin types III and IV) with acne scars received up to five treatments of combined fractional 915‐nm laser and bipolar RF using a Matrix IR applicator (Syneron Medical Ltd, Yokneam, Israel) with fluence ranging from 50 to 70 J/cm2, RF at 70–100 J/cm3, double passes followed by full‐face bipolar fractional RF treatment using Matrix RF at energy ranging from 50 to 62 mJ/pin, at 4‐week intervals. Changes in acne scars, skin texture, pore size, pigmentation irregularity, and complications were assessed up to 3 months post‐treatment by standardized photographs obtained with Canfield Visia‐CR system®. Subjective improvement and patient satisfaction were assessed by questionnaire.ResultsTwenty patients (age 27.7 ± 8.4 years) completed the study. Modest but statistically significant improvement was noted in acne scars, with the mean grade decreased by 29% (P < 0.001), and 52% were rated with at least moderate objective global improvement at 3 months. Mean pain score was 2.6 on a scale of 0–4. There were also objective improvements in all secondary endpoints. Post‐inflammatory hyperpigmentation (PIH) occurred mainly over bony areas in 6.5% of all treatments. Subjective improvement was moderate to significant for 36.8% of patients, and 63% reported being satisfied with the treatment results at 3 months despite considerable pain level.ConclusionUse of fractional laser with RF followed by fractional RF was shown to be safe and effective for acne scars with modest improvement and low PIH rate comparable to other resurfacing techniques in this Asian case series. Adequate pain control and recduced energy level when treating areas in close proximity to bone are advised. Lasers Surg. Med. 44: 622–630, 2012. © 2012 Wiley Periodicals, Inc.
Exact solution for granular flows
Authors: Faccanoni Gloria , Mangeney Anne 
Published: August 2012

SUMMARYIn this paper, we present the exact solution of the Riemann problem for the nonlinear one‐dimensional so‐called shallow‐water or Saint‐Venant equations with friction proposed by SAVAGE and HUTTER to describe debris avalanches. This model is based on the depth‐averaged thin layer approximation of granular flows over sloping beds and takes into account a Coulomb type friction law with a constant friction coefficient. A particular configuration of the Riemann problem corresponds to a dam of infinite length in one direction from which granular material is released from rest at a given time over an inclined rigid or erodible bed. We solve analytically and numerically the depth‐averaged long‐wave equations derived in a topography‐linked coordinate system for all the possible Riemann problems. The detailed mathematical proof of the derivation of the analytical solutions and the analysis of their structure and properties is intended, first of all, for geophysicists, mathematicians, and physicists because of the possible extension of this study to more complex problems (geometries, friction laws, …). The numerical solution of the first‐order finite‐volume method based on a Godunov‐type scheme is compared with the proposed exact Riemann problem solution. This solution is used to solve the dam‐break problem and analyze the influence of the thickness of the erodible bed on the speed of the granular front. Comparison with existing experimental results shows that, for an erodible bed, the equations lack fundamental physical significance to reproduce the observed dynamics of erosive granular flows. Copyright © 2012 John Wiley & Sons, Ltd.
Molecular characterization of mucinous ovarian tumours supports a stratified treatment approach with HER2 targeting in 19% of carcinomas
Authors: Anglesio Michael S , Kommoss Stefan , Tolcher Mary C , Clarke Blaise , Galletta Laura , Porter Henry , Damaraju Sambasivarao , Fereday Sian , Winterhoff Boris J , Kalloger Steve E , Senz Janine , Yang Winnie , Steed Helen , Allo Ghassan , Ferguson Sarah , Shaw Patricia , Teoman Attila , Garcia Joaquin J , Schoolmeester John K , Bakkum‐Gamez Jamie , Tinker Anna V , Bowtell David D , Huntsman David G , Gilks C Blake , McAlpine Jessica N 
Published: August 2012

AbstractMucinous ovarian carcinomas (MCs) typically do not respond to current conventional therapy. We have previously demonstrated amplification of HER2 in 6 of 33 (18.2%) mucinous ovarian carcinomas (MCs) and presented anecdotal evidence of response with HER2‐targeted treatment in a small series of women with recurrent HER2‐amplified (HER2+) MC. Here, we explore HER2 amplification and KRAS mutation status in an independent cohort of 189 MCs and 199 mucinous borderline ovarian tumours (MBOTs) and their association to clinicopathological features. HER2 status was assessed by immunohistochemistry (IHC), FISH, and CISH, and interpreted per ASCO/CAP guidelines, with intratumoural heterogeneity assessment on full sections, where available. KRAS mutation testing was performed with Sanger sequencing. Stage and grade were associated with recurrence on both univariate and multivariate analysis (p < 0.001). Assessment of HER2 status revealed overexpression/amplification of HER2 in 29/154 (18.8%) MCs and 11/176 (6.2%) MBOTs. There was excellent agreement between IHC, FISH, and CISH assessment of HER2 status (perfect concordance of HER2 0 or 1+ IHC with non‐amplified status, and 3+ IHC with amplified status). KRAS mutations were seen in 31/71 (43.6%) MCs and 26/33 (78.8%) MBOTs, and were near mutually exclusive of HER2 amplification. In the 189 MC cases, a total of 54 recurrences and 59 deaths (53 of progressive disease) were observed. Within MCs, either HER2 amplification/overexpression or KRAS mutation was associated with decreased likelihood of disease recurrence (p = 0.019) or death (p = 0.0041) when compared to cases with neither feature. Intratumoural heterogeneity was noted in 26% of HER2‐overexpressing cases. These data support the stratification of MCs for the testing of new treatments, with HER2‐targeted therapy as a viable option for HER2+ advanced or recurrent disease. Further research is required to delineate the molecular and clinical features of the ∼34% of MC cases with neither HER2 amplification nor KRAS mutations.
Vapor phase oxidative synthesis of conjugated polymers and applications
Authors: Bhattacharyya Dhiman , Howden Rachel M. , Borrelli David C. , Gleason Karen K. 
Published: August 2012

AbstractSince their discovery, electrically conductive polymers have gained immense interest both in the fields of basic and applied research. Despite their vast potential in the fabrication of efficient, flexible, and low‐cost electronic and optoelectronic devices, they are often difficult to process by wet‐chemical methods due to their very low to poor solubility in organic solvents. The use of vapor‐based synthetic routes, in which conductive polymers can be synthesized and deposited as a thin film directly on a substrate from the vapor phase, provides many unique advantages. This article discusses oxidative vapor deposition processes, primarily vapor phase polymerization and oxidative chemical vapor deposition, of conjugated polymers and their applications. The mild operating conditions (near room temperature processing) allow conformal and functional coatings of conjugated polymers on delicate substrates. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012