bluuebunny/crossref_metadata_2025_split huggingface dataset:
Codistribution of Collagen Type IV and Laminin in Liver Fibrosis of Elderly Cadavers: Immunohistochemical Marker of Perisinusoidal Basement Membrane Formation
Authors: Mak Ki M. , Chen Lucy L. , Lee Ting‐Fang 
Published: June 2014

ABSTRACTLiver sinusoids are lined by a fenestrated endothelium that lacks a basement membrane. Formation of perisinusoidal basement membranes beneath the endothelium is an integral feature of capillarization of sinusoids that is a significant pathology found in advanced fibrosis. Liver fibrosis is prevalent in elderly cadavers; however, basement membrane formation in these liver samples has yet to be studied. Collagen type IV and laminin are major basement membrane proteins and their codistribution around sinusoids provides an immunohistochemical marker of basement membrane formation. Here, we examined the intralobular sites of perisinusoidal basement membrane formation in elderly cadaveric livers having various stages of fibrosis. Collagen IV and laminin codistributed in basement membranes of portal and septal ductular and vascular structures, providing a positive control. In the parenchyma, collagen IV immunostaining of sinusoids was panlobular in all stages of fibrosis, and the stain was continuous along the sinusoids. In contrast, laminin was not detected in livers, showing minimal fibrotic change. It was rarely seen in perisinusoidal/pericellular fibrosis, but frequently in septa formation, bridging fibrosis, and cirrhosis. The laminin stain was patchy, occurring principally in sinusoids of periportal and periseptal areas, less commonly in mid‐lobular and rarely in centrilobular areas. Consecutive sections revealed that laminin codistributed with collagen IV in these sinusoidal locations, thus marking the sites of perisinusoidal basement membrane formation in aged fibrotic livers. This development is presumably related to aging of the liver and exacerbated by liver injury caused by advanced liver fibrosis, possibly resulting in sinusoidal capillarization. Anat Rec, 296:953–964, 2013. © 2013 Wiley Periodicals, Inc.
The Shape and Presentation of the Catarrhine Talus: A Geometric Morphometric Analysis
Authors: Turley Kevin , Frost Stephen R. 
Published: June 2014

ABSTRACTThe distal component of the talo‐crural joint, the talus, was compared, using geometric morphometrics, in 219 specimens from nine extant taxa to identify differences in shape and the factors influencing them. The specimens were laser scanned, digitally reconstructed, and landmarked. The whole talus, proximal and distal articular facet subgroups were analyzed using Generalized Procrustes analysis, linear regression, principal component analysis, analysis of percent variance, dot‐product vector analysis, and pair‐wise permutation tests to evaluate shape, and were visualized by TPS deformation of an exemplar surface. Significant percentages of shape variation among taxa were due to body mass, talar size, superfamily, and substrate preference. Shape and presentational morphology associated with these factors were documented, along with the similarities and differences among individual taxa. Nearly all taxa were significantly different in overall, proximal and distal shapes. The most important factors influencing whole talar shape were log centroid size and substrate preference. Substrate preference was also the most important factor defining proximal articular morphology and unrelated to other factor such as mass, while distal articular morphology was influenced by superfamily (head angle and shape). Results demonstrated that substrate preference and superfamily significantly influenced distal presentation, while substrate preference influenced proximal articular shape. Anat Rec, 296:877–890, 2013. © 2013 Wiley Periodicals, Inc.
Cranial Muscle Development in the Model Organism Ambystoma mexicanum: Implications for Tetrapod and Vertebrate Comparative and Evolutionary Morphology and Notes on Ontogeny and Phylogeny
Authors: Ziermann Janine M. , Diogo Rui 
Published: June 2014

ABSTRACTThere is still confusion about the homology of several cranial muscles in salamanders with those of other vertebrates. This is true, in part, because of the fact that many muscles present in early ontogeny of amphibians disappear during development and specifically during metamorphosis. Resolving this confusion is important for the understanding of the comparative and evolutionary morphology of vertebrates and tetrapods because amphibians are the phylogenetically most plesiomorphic tetrapods, concerning for example their myology, and include two often used model organisms, Xenopus laevis (anuran) and Ambystoma mexicanum (urodele). Here we provide the first detailed report of the cranial muscle development in axolotl from early ontogenetic stages to the adult stage. We describe different and complementary types of general muscle morphogenetic gradients in the head: from anterior to posterior, from lateral to medial, and from origin to insertion. Furthermore, even during the development of neotenic salamanders such as axolotls, various larval muscles become indistinct, contradicting the commonly accepted view that during ontogeny the tendency is mostly toward the differentiation of muscles. We provide an updated comparison between these muscles and the muscles of other vertebrates, a discussion of the homologies and evolution, and show that the order in which the muscles appear during axolotl ontogeny is in general similar to their appearance in phylogeny (e.g. differentiation of adductor mandibulae muscles from one anlage to four muscles), with only a few remarkable exceptions, as for example the dilatator laryngis that appears evolutionary later but in the development before the intermandibularis. Anat Rec, 296:1031–1048, 2013. © 2013 Wiley Periodicals, Inc.
Accessible and Informative Sectioned Images, Color‐Coded Images, and Surface Models of the Ear
Authors: Park Hyo Seok , Chung Min Suk , Shin Dong Sun , Jung Yong Wook , Park Jin Seo 
Published: June 2014

ABSTRACTIn our previous research, we created state‐of‐the‐art sectioned images, color‐coded images, and surface models of the human ear. Our ear data would be more beneficial and informative if they were more easily accessible. Therefore, the purpose of this study was to distribute the browsing software and the PDF file in which ear images are to be readily obtainable and freely explored. Another goal was to inform other researchers of our methods for establishing the browsing software and the PDF file. To achieve this, sectioned images and color‐coded images of ear were prepared (voxel size 0.1 mm). In the color‐coded images, structures related to hearing, equilibrium, and structures originated from the first and second pharyngeal arches were segmented supplementarily. The sectioned and color‐coded images of right ear were added to the browsing software, which displayed the images serially along with structure names. The surface models were reconstructed to be combined into the PDF file where they could be freely manipulated. Using the browsing software and PDF file, sectional and three‐dimensional shapes of ear structures could be comprehended in detail. Furthermore, using the PDF file, clinical knowledge could be identified through virtual otoscopy. Therefore, the presented educational tools will be helpful to medical students and otologists by improving their knowledge of ear anatomy. The browsing software and PDF file can be downloaded without charge and registration at our homepage (http://anatomy.dongguk.ac.kr/ear/). Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.
Is Mda‐7/IL‐24 a Potential Target and Biomarker for Enhancing Drug Sensitivity in Human Glioma U87 Cell Line?
Authors: Wang Qin , Zhu Yu , Yang Ping 
Published: June 2014

ABSTRACTGliomas are the most common form of primary brain tumor with the highest mortality rates. Drug resistance is a major cause of treatment failure in patients with glioma. Melanoma differentiation associated gene‐7/interleukin‐24 (mda‐7/IL‐24) has been demonstrated to play an important role in drug resistance in human cancer cell lines. However, the reversing effect of mda‐7/IL‐24 on drug resistance of human glioma is not fully clear. Here, we investigated the effects of overexpression of the mda‐7/IL‐24 gene in human glioma. We established a cisplatin‐resistant U87 glioma cell line and found that mda‐7/IL‐24 was highly correlated with drug resistance. Furthermore, we investigated the apoptotic rate, intracellular accumulation of Rhodamine‐123, and expression of glutathione and P‐glycoprotein. The over‐expression of mda‐7/IL‐24 enhanced cisplatin cytotoxicity and reversal of drug resistance in glioma cells. The reversing effect of mda‐7/IL‐24 on drug resistance was induced mainly through the regulation of drug resistance‐related genes and efflux drug pumps. Thus, mda‐7/IL‐24 can be used as a promising predictive biomarker and potential therapeutic target for chemotherapy in glioma. Anat Rec, 2013. © 2013 Wiley Periodicals, Inc.
Odorant‐Binding Proteins and Xenobiotic Metabolizing Enzymes: Implications in Olfactory Perireceptor Events
Authors: Heydel Jean‐Marie , Coelho Alexandra , Thiebaud Nicolas , Legendre Arièle , Bon Anne‐Marie Le , Faure Philippe , Neiers Fabrice , Artur Yves , Golebiowski Jérôme , Briand Loïc 
Published: June 2014

ABSTRACTAt the periphery of the olfactory system, the binding of odorants on olfactory receptors (ORs) is usually thought to be the first level of the perception of smell. However, at this stage, there is evidence that other molecular mechanisms also interfere with this chemoreception by ORs. These perireceptor events are mainly supported by two groups of proteins present in the olfactory nasal mucus or in the nasal epithelium. Odorant‐binding proteins (OBPs), the first group of proteins have been investigated for many years. OBPs are small carrier proteins capable of binding odorants with affinities in the micromolar range. Although there is no absolute evidence to support their functional roles in vertebrates, OBPs are good candidates for the transport of inhaled odorants towards the ORs via the nasal mucus. The second group of proteins involves xenobiotic metabolizing enzymes, which are strongly expressed in the olfactory epithelium and supposed to be involved in odorant transformation, degradation, and/or olfactory signal termination. Following an overview of these proteins, this review explores their roles, which are still a matter of debate. Anat Rec, 296:1333‐1345, 2013. © 2013 Wiley Periodicals, Inc.
The Olfactory System as a Puzzle: Playing With Its Pieces
Authors: Díaz D. , Gómez C. , Muñoz‐Castañeda R. , Baltanás F. , Alonso J. R. , Weruaga E. 
Published: June 2014

AbstractThe mammalian olfactory bulb (OB) has all the features of a whole mammalian brain but in a more reduced space: neuronal lamination, sensory inputs, afferences, or efferences to other centers of the central nervous system, or a contribution of new neural elements. Therefore, it is widely considered as “a brain inside the brain.” Although this rostral region has the same origin and general layering as the other cerebral cortices, some distinctive features make it very profitable in experimentation in neurobiology: the sensory inputs are driven directly on its surface, the main output can be accessed anatomically, and new elements appear in it throughout adult life. These three morphological characteristics have been manipulated to analyze further the response of the whole OB. The present review offers a general outlook into the consequences of such experimentation in the anatomy, connectivity and neurochemistry of the OB after (a) sensory deprivation, mainly by naris occlusion; (b) olfactory deinnervation by means of olfactory epithelium damage, olfactory nerve interruption, or even olfactory tract disruption; (c) the removal of the principal neurons of the OB; and (d) management of the arrival of newborn interneurons from the rostral migratory stream. These experiments were performed using surgical or chemical methods, but also by means of the analysis of genetic models, some of whose olfactory components are missing, colorless or mismatching within the wild‐type scenario of odor processing. Anat Rec, 296:1383‐1400, 2013. © 2013 Wiley Periodicals, Inc.
Exotic Models May Offer Unique Opportunities to Decipher Specific Scientific Question: The Case of Xenopus Olfactory System
Authors: Gascuel Jean , Amano Tosikazu 
Published: June 2014

ABSTRACTThe fact that olfactory systems are highly conserved in all animal species from insects to mammals allow the generalization of findings from one species to another. Most of our knowledge about the anatomy and physiology of the olfactory system comes from data obtained in a very limited number of biological models such as rodents, Zebrafish, Drosophila, and a worm, Caenorhabditis elegans. These models have proved useful to answer most questions in the field of olfaction, and thus concentrating on these few models appear to be a pragmatic strategy. However, the diversity of the organization and physiology of the olfactory system amongst phyla appear to be greater than generally assumed and the four models alone may not be sufficient to address all the questions arising from the study of olfaction. In this article, we will illustrate the idea that we should take advantage of biological diversity to address specific scientific questions and will show that the Xenopus olfactory system is a very good model to investigate: first, olfaction in aerial versus aquatic conditions and second, mechanisms underlying postnatal reorganization of the olfactory system especially those controlled by tyroxine hormone. Anat Rec, 296:1453‐1461, 2013. © 2013 Wiley Periodicals, Inc.
A Cytological and Experimental Study of the Neuropil and Primary Olfactory Afferences to the Piriform Cortex
Authors: Vargas‐Barroso Víctor , Larriva‐Sahd Jorge 
Published: June 2014

ABSTRACTThe microscopic organization of the piriform cortex (PC) was studied in normal and experimental material from adult albino rats. In rapid‐Golgi specimens a set of collaterals from the lateral olfactory tract (i.e., sublayer Ia) to the neuropil of the Layer II (LII) was identified. Specimens from experimental animals that received electrolytic lesion of the main olfactory bulb three days before sacrificing, were further processed for pre‐embedding immunocytochemistry to the enzyme glutamic acid decarboxylase 67 (GAD 67). This novel approach permitted a simultaneous visualization at electron microscopy of both synaptic degeneration and GAD67‐immunoreactive (GAD‐I) sites. Degenerating and GAD‐I synapses were separately found in the neuropil of Layers I and II of the PC. Previously overlooked patches of neuropil were featured in sublayer Ia. These areas consisted of dendritic and axonal processes including four synaptic types. Tridimensional reconstructions from serial thin sections from LI revealed the external appearance of the varicose and tubular dendrites as well as the synaptic terminals therein. The putative source(s) of processes to the neuropil of sublayer Ia is discussed in the context of the internal circuitry of the PC and an alternative model is introduced Anat Rec, 296:1297‐1316, 2013. © 2013 Wiley Periodicals, Inc.
Characterization of Growth, Glomerular Number, and Tubular Proteins in the Developing Rhesus Monkey Kidney
Authors: Batchelder Cynthia A. , Keyser Jennifer L. , Lee C. Chang I. , Tarantal Alice F. 
Published: June 2014

ABSTRACTAn essential step in the translation of cell‐based therapies for kidney repair involves preclinical studies in relevant animal models. Regenerative therapies in children with congenital kidney disease may provide benefit, but limited quantitative data on normal development is available to aid in identifying efficient protocols for repair. Nonhuman primates share many developmental similarities with humans and provide an important translational model for understanding nephrogenesis and morphological changes across gestation. These studies assessed monkey kidney size and weight during development and utilized stereological methods to quantitate total number of glomeruli. Immunohistochemical methods were included to identify patterns of expression of tubular proteins including Aquaporin‐1 (AQP1), AQP2, Calbindin, E‐Cadherin, and Uromodulin. Results have shown that glomerular number increased linearly with kidney weight, from 1.1 × 103 in the late first trimester to 3.5 × 105 near term (P < 0.001). The ratio of glomeruli to body weight tripled from the late first to early second trimester then remained relatively unchanged. Only AQP1 was expressed in the proximal tubule and descending Loop of Henle. The ascending Loop of Henle was positive for AQP2, Calbindin, and Uromodulin; distal convoluted tubules stained for Calbindin only; and collecting tubules expressed AQP2 and E‐Cadherin with occasional Calbindin‐positive cells. These findings provide quantitative information on normal kidney ontogeny in rhesus monkeys and further support the importance of this model for human kidney development. Anat Rec, 296:1747–1757, 2013. © 2013 Wiley Periodicals, Inc.
Sensory ability in the narwhal tooth organ system
Authors: Nweeia Martin T. , Eichmiller Frederick C. , Hauschka Peter V. , Donahue Gretchen A. , Orr Jack R. , Ferguson Steven H. , Watt Cortney A. , Mead James G. , Potter Charles W. , Dietz Rune , Giuseppetti Anthony A. , Black Sandie R. , Trachtenberg Alexander J. , Kuo Winston P. 
Published: June 2014

ABSTRACTThe erupted tusk of the narwhal exhibits sensory ability. The hypothesized sensory pathway begins with ocean water entering through cementum channels to a network of patent dentinal tubules extending from the dentinocementum junction to the inner pulpal wall. Circumpulpal sensory structures then signal pulpal nerves terminating near the base of the tusk. The maxillary division of the fifth cranial nerve then transmits this sensory information to the brain. This sensory pathway was first described in published results of patent dentinal tubules, and evidence from dissection of tusk nerve connection via the maxillary division of the fifth cranial nerve to the brain. New evidence presented here indicates that the patent dentinal tubules communicate with open channels through a porous cementum from the ocean environment. The ability of pulpal tissue to react to external stimuli is supported by immunohistochemical detection of neuronal markers in the pulp and gene expression of pulpal sensory nerve tissue. Final confirmation of sensory ability is demonstrated by significant changes in heart rate when alternating solutions of high‐salt and fresh water are exposed to the external tusk surface. Additional supporting information for function includes new observations of dentinal tubule networks evident in unerupted tusks, female erupted tusks, and vestigial teeth. New findings of sexual foraging divergence documented by stable isotope and fatty acid results add to the discussion of the functional significance of the narwhal tusk. The combined evidence suggests multiple tusk functions may have driven the tooth organ system's evolutionary development and persistence. Anat Rec, 297:599–617, 2014. © 2014 Wiley Periodicals, Inc.
Patterns of Morphological Variation of Extant Sloth Skulls and their Implication for Future Conservation Efforts
Authors: Hautier Lionel , Billet Guillaume , Eastwood Bethany , Lane Jemima 
Published: June 2014

ABSTRACTSeveral studies have shown an increased morphological variability of sloths from mammalian norms, affecting varied phenotypic traits from skeletal parts to soft tissues. We present here the first descriptive comparison of the whole skull morphology within the two extant sloth genera, combining geometric morphometric approaches with comparative anatomy. We used these methods to explore the patterns of the intra‐ and interspecific morphological variation of the skull with regard to several factors such as phylogeny, geography, allometry, or sexual dimorphism. Our study first revealed strong phylogenetic and geographical imprints on the cranial and mandibular morphological traits. This result demonstrates the importance of accurate knowledge of species and their geographical distributions; here we show from an example pertaining to Bradypus variegatus populations the implications this has on conservation management. Moreover, in order to control the amount of this detected variation, we tentatively compared sloths to a wide range of mammalian species. Our analysis found no significant increase in the average deviation of skull shape within each investigated sloth species compared to other mammals. This suggests that the intraspecific cranial variation in sloths does not depart significantly from the variation observed in other mammals. This result has positive implications for the demarcation of anatomical regions that maintain high levels of morphological variation in sloths. Anat Rec, 297:979–1008, 2014. © 2014 Wiley Periodicals, Inc.
Regional and Epi‐ to Endocardial Differences in Transmural Angles of Left Ventricular Cardiomyocytes Measured in Ex Vivo Pig Hearts: Functional Implications
Authors: Smerup Morten , Agger Peter , Nielsen Eva Amalie , Ringgaard Steffen , Pedersen Michael , Niederer Peter , Anderson Robert H. , Lunkenheimer Paul P. 
Published: June 2014

ABSTRACTRecent studies point toward the existence of a significant population of cardiomyocytes that intrude transmurally, in addition to those aligned tangentially. Our aim was to investigate the extent of transmural angulation in the porcine left ventricle using diffusion tensor magnetic resonance imaging (DTMRI). Hearts from eight 15 kg pigs were arrested in diastole. The ventricles were filled with polymer to maintain the end‐diastolic dimensions. All hearts were examined using DTMRI to assess the distribution of transmural angulation of the cardiomyocytes at 12 predetermined locations covering the entirety of the left ventricle. We found significant differences between the regions, as well as within the transmural subcomponents. In eight out of the 12 predetermined mural segments, the highest mean transmural angle was located sub‐endocardially. The greatest mean transmural angles were found in the anterior basal region, specifically 14.9 ± 6.0‐degree angle, with the greatest absolute value being 34.3‐degree angle. This is the first study to show the significant heterogeneities in the distribution of helical and transmural angles within the entirety of the left ventricular walls, not only for different depths within the ventricular walls, but also between different ventricular regions. The results show unequivocally that not all the contractile elements are aligned exclusively in tangential fashion within the left ventricle. The main function of the transmurally intruding component is most likely to equalize and normalize shortening of the cardiomyocytes at all depths within the myocardium, but our findings also support the notion of antagonistic forces existing within the myocardial walls. Anat Rec, 296:1724–1734, 2013. © 2013 Wiley Periodicals, Inc.