bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activation of coagulation and platelets by candidate membranes of implantable devices in a whole blood model without soluble anticoagulant
Authors: Sokolov A. , Hellerud B. C. , Tønnessen T. I. , Johannessen E. A. , Mollnes T. E. 
Published: September 2012

AbstractImplantable devices are challenged with thrombus formation at their biomaterial interface. Thus the importance of identifying compatible biomaterials that will help to improve the performance of these devices are becoming increasingly paramount. The aim of this study was to evaluate the activation of coagulation and platelets by candidate membranes considered for use in implantable devices on the basis of an adapted whole blood model without soluble anticoagulants. Evaluated materials were incubated with whole blood without soluble anticoagulant in wells coated with heparin. Prothrombin fragment 1+2 (PTF 1+2), thrombin‐antithrombin complex (TAT), and β‐thromboglobulin (BTG) were analyzed in plasma samples using enzyme immunoassays. The C5 inhibitor eculizumab was used to evaluate the role of complement. Incubation of two of the polyamide membranes PAR and PATF led to an increase in concentration of PTF 1+2 and TAT (p < 0.01 for PAR, ns for PATF). The BTG concentration was significantly increased for five materials [PAR, PATF, polycarbonate (PC), and two polyarylethersulphone membranes PAES‐1 and PAES‐2]. Complement inhibition had no effect on coagulation or platelet activation induced by PAR and PATF. In conclusion, PAR and PATF were not compatible with blood and should be avoided for use in implantable devices. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Cell fate conversion: Direct induction of hepatocyte‐like cells from fibroblasts
Authors: Ji Shuyi , Zhang Ludi , Hui Lijian 
Published: September 2012

AbstractOne of the essential features of stem cells is their cellular plasticity to differentiate into daughter cells with defined functions. Recently, induction of pluripotent stem cells from somatic cells by defined transcription factors led to the focus on cellular plasticity of terminally differentiated cells. This approach is adopted by other studies to demonstrate the cell fate conversion between different lineages of terminally differentiated cells. We and others showed that induced hepatocyte‐like (iHep) cells are directly converted from mouse fibroblasts by overexpression of liver‐enriched transcription factors. iHep cells as well as pluripotent stem cell‐ or mesenchymal stem cell‐derived hepatocyte‐like cells provide potential cell sources for disease modeling, transplantation, and tissue engineering independent of donor organs. Here, we review the latest advances in generating hepatocyte‐like cells and summarize general criteria for evaluating these cells. In addition, we propose a possible role of the p19Arf/p53 pathway in cell fate maintenance, which apparently limits the formation of induced pluripotent stem (iPS) cells and iHep cells. J. Cell. Biochem. 114: 256–265, 2013. © 2012 Wiley Periodicals, Inc.
The cancer‐related transcription factor Runx2 modulates cell proliferation in human osteosarcoma cell lines
Authors: Lucero Claudia M.J. , Vega Oscar A. , Osorio Mariana M. , Tapia Julio C. , Antonelli Marcelo , Stein Gary S. , van Wijnen Andre J. , Galindo Mario A. 
Published: September 2012

AbstractRunx2 regulates osteogenic differentiation and bone formation, but also suppresses pre‐osteoblast proliferation by affecting cell cycle progression in the G1 phase. The growth suppressive potential of Runx2 is normally inactivated in part by protein destabilization, which permits cell cycle progression beyond the G1/S phase transition, and Runx2 is again up‐regulated after mitosis. Runx2 expression also correlates with metastasis and poor chemotherapy response in osteosarcoma. Here we show that six human osteosarcoma cell lines (SaOS, MG63, U2OS, HOS, G292, and 143B) have different growth rates, which is consistent with differences in the lengths of the cell cycle. Runx2 protein levels are cell cycle‐regulated with respect to the G1/S phase transition in U2OS, HOS, G292, and 143B cells. In contrast, Runx2 protein levels are constitutively expressed during the cell cycle in SaOS and MG63 cells. Forced expression of Runx2 suppresses growth in all cell lines indicating that accumulation of Runx2 in excess of its pre‐established levels in a given cell type triggers one or more anti‐proliferative pathways in osteosarcoma cells. Thus, regulatory mechanisms controlling Runx2 expression in osteosarcoma cells must balance Runx2 protein levels to promote its putative oncogenic functions, while avoiding suppression of bone tumor growth. J. Cell. Physiol. 228: 714–723, 2013. © 2012 Wiley Periodicals, Inc.
IGF binding protein‐3 mediates stress‐induced apoptosis in non‐transformed mammary epithelial cells
Authors: Leibowitz Brian J. , Agostini‐Dreyer Allyson , Jetzt Amanda E. , Krumm Christopher S. , Cohick Wendie S. 
Published: September 2012

AbstractMammary epithelial cell (MEC) number is an important determinant of milk production in lactating dairy cows. IGF‐I increases IGF binding protein‐3 (IGFBP‐3) production in these cells, which plays a role in its ability to enhance proliferation. In the present study, we show that the apoptotic factor anisomycin (ANS) also increases IGFBP‐3 mRNA and protein in a dose‐ and concentration‐dependent manner that mirrors activation of caspase‐3 and ‐7, with significant increases in both IGFBP‐3 protein and caspase activation observed by 3 h. Knock‐down of IGFBP‐3 with small interfering (si) RNA attenuated the ability of ANS to induce apoptosis, while knock‐down of IGFBP‐2, the other major IGFBP made by bovine MEC, had no effect. Reducing IGFBP‐3 also decreased the ability of ANS to induce mitochondrial cytochrome c release, indicating its involvement in the intrinsic apoptotic pathway. In contrast, transfection with IGFBP‐3 in the absence of ANS failed to induce apoptosis. Since both the mitogen IGF‐I and the apoptotic inducer ANS increase IGFBP‐3 production in MEC, we proposed that cellular localization might determine IGFBP‐3 action. While both IGF‐I and ANS stimulated the release of IGFBP‐3 into conditioned media, only ANS induced nuclear localization of IGFBP‐3. A pan‐caspase inhibitor had no effect on ANS‐induced nuclear localization of IGFBP‐3, indicating that nuclear entry of IGFBP‐3 precedes caspase activation. Treatment with IGF‐I had no effect on ANS‐induced nuclear localization, but did block ANS‐induced apoptosis. In summary, our data indicate that IGFBP‐3 plays a role in stress‐induced apoptosis that may require nuclear localization in non‐transformed MEC. J. Cell. Physiol. 228: 734–742, 2013. © 2012 Wiley Periodicals, Inc.
A Kinetic Study on Catechol‐Based Belousov–Zhabotinsky Reaction
Authors: Shah Ishfaq Ahmad , Peerzada G. M. , Ganaie Nadeem B. , Dar Nisar A. 
Published: September 2012

AbstractThe present investigation pertains to the kinetic study on bromate‐driven and manganese ion catalyzed Belousov–Zhabotinsky oscillatory chemical reaction having catechol (1,2‐dihydroxybenzene) as the organic substrate in aqueous acid media at 30 ± 0.1oC under stirred conditions. Although some polyphenols as chemical oscillators are known in the literature but the role of catechol has been marginalized. The reaction system with catechol shows long‐time series of periodic as well as aperiodic oscillations, which were monitored potentiometrically in the oxidation reduction potential (ORP) mode under batch conditions. The various oscillatory parameters such as induction time (tin), time period (tp), amplitude (A), and number of oscillations (N) were obtained by drawing a plot between redox potential (mV) versus time (s). It is found that the oscillatory parameters of the system vary with the concentration of the reacting species. The reaction system was also studied in different aqueous acid media, but the detailed studies with respect to experimental parameters have been made in aqueous sulfuric acid medium as it showed a broad oscillatory window and better oscillatory characteristics. Moreover, the oscillatory parameters in general and induction time in particular vary significantly with temperature (15 to 45 ± 0.1oC). The activation parameters of the system have also been calculated. The oscillatory behavior of the reaction system was also confirmed spectrophotometrically. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 45: 141–151, 2013
Droplet‐in‐Droplet Polymer Blend Microstructures: a Potential Route Toward the Recycling of Co‐mingled Plastics
Authors: Le Corroller Pierre , Favis Basil D. 
Published: September 2012

AbstractThe problem of recycling co‐mingled plastics is primarily related to the problem of multiple interfaces in multicomponent immiscible polymer blends. Here, we study a new concept where thermodynamically driven polymer segregation and phase encapsulation are used to locate multiple phases within one of two major phases. Starting from a co‐continuous blend of HDPE and PP, hierarchically encapsulated PS/PMMA/PC composite droplets are found to be exclusively located within PP when the principal HDPE/PP interface is compatibilized. The mechanical properties of the structures demonstrate an improved tensile strength and modulus as well as ductility under the appropriate processing conditions. This approach has potential as a novel route toward the recycling of co‐mingled plastics.
Role of cyclin B1/Cdc2 in mediating Bcl‐XL phosphorylation and apoptotic cell death following nocodazole‐induced mitotic arrest
Authors: Choi Hye Joung , Zhu Bao Ting 
Published: September 2012

AbstractTreatment of cancer cells with microtubule inhibitors causes mitotic arrest, which subsequently leads to cell death via activation of the intrinsic apoptotic pathway. Mitotically arrested cells typically display increased phosphorylation (i.e., inactivation) of two key anti‐apoptotic proteins, Bcl‐2 and Bcl‐XL, but the mechanisms that regulate their phosphorylation as well as their role in apoptotic cell death following mitotic arrest are still poorly understood at present, which are the focus of this study. We recently showed that cyclin B1 and cell division cycle 2 (Cdc2) proteins are strongly up‐regulated in human breast cancer cells following treatment with nocodazole (a prototypical microtubule inhibitor), and their up‐regulation plays a critical role in the development of mitotic prometaphase arrest. In this study, we present evidence showing that the up‐regulated cyclin B1/Cdc2 complex in nocodazole‐treated human breast cancer cells is also responsible for the increased phosphorylation of Bcl‐2 and Bcl‐XL. However, only the increased phosphorylation of Bcl‐XL, but not the phosphorylation of Bcl‐2, contributes to subsequent activation of the intrinsic cell death pathway. In addition, evidence is presented to show that mitotic arrest deficient 2 (MAD2) is a key upstream mediator of the up‐regulation of cyclin B1/Cdc2 as well as the subsequent increase in phosphorylationof Bcl‐2 and Bcl‐XL in nocodazole‐treated cancer cells. Together, these results reveal that the up‐regulated cyclin B1/Cdc2 complex not only mediates prometaphase arrest in nocodazole‐treated cells, but also activates the subsequent intrinsic cell death pathway in these cells via increased phosphorylation of Bcl‐XL. © 2012 Wiley Periodicals, Inc.
Proteomic profiling of osteosarcoma cells identifies ALDOA and SULT1A3 as negative survival markers of human osteosarcoma
Authors: Chen Xiang , Yang Tong‐Tao , Zhou Yong , Wang Wei , Qiu Xiu‐Chun , Gao Jie , Li Cun‐Xiao , Long Hua , Ma Bao‐An , Ma Qiong , Zhang Xian‐zhi , Yang Lian‐Jia , Fan Qing‐Yu 
Published: September 2012

AbstractOsteosarcoma (OSA) is the most common primary malignancy of bone. Molecular mechanism underlying OSA remains to be fully elucidated. It is critical to identify reliable diagnostic and prognostic markers for OSA at the molecular levels. This study is designed to investigate possible molecular mechanisms behind OSA development and to identify novel prognostic markers related to OSA survival. We conduct a comprehensive proteomic profiling analysis of human OSA cell lines with differential metastatic potential. Through comprehensive combinatorial analyses of the proteomic data and the previously obtained cDNA microarray results, we identify 37 candidate proteins which are differentially expressed in OSA sublines. Among them, ALDOA and SULT1A3 are selected for further investigation. The expressions of protein are confirmed by Western blotting analysis. We further analyze the expression levels of ALDOA and SULT1A3 from 40 clinical cases of OSA. The results demonstrate that the expression of ALDOA and/or SULT1A3 is significantly higher in patients with worse survival time than patients with better survival time. Five‐year survival analysis shows there is a statistically significant difference between two patient populations. The data strongly suggest that ALDOA and/or SULT1A3 expression level in biopsy samples may predict the clinical outcomes of OSA patients. Furthermore, the biological functions of ALDOA and SULT1A3 may be implicated in OSA development and/or progression. © 2012 Wiley Periodicals, Inc.
Selected nutraceutic screening by therapeutic effects on doxorubicin‐induced chronic kidney disease
Authors: Peng Chiung‐Chi , Hsieh Chiu‐Lan , Ker Yaw‐Bee , Wang Hsi‐Yi , Chen Kuan‐Chou , Peng Robert Y. 
Published: September 2012

ScopeThe number of patients with chronic kidney disease (CKD) are increasing. Interventions such as controlling hypertension and specific pharmacologic options are recommended. Some nutraceutics may have benefits in this regard.Methods and resultsNaringenin (a flavanon), catechin (a flavanol), and quercetin (a flavonol) and rutin (a flavonol rutinoside) were tried on CKD in a Sprague Dawley rat model. Results indicated quercetin to be the most effective therapeutic candidate with respect to renal edema, hypertension, serum creatinine, hematocrit, cardiopathy, aorta calcification, glomerular amyloidosis, erythrocyte depletion in bone marrow, collagen deposition, expressions of TNF‐α, cleaved caspase‐3, IκBα, PPARα, and serum insulin. But quercetin was only partially effective in restoring glomerular filtration rate, albuminuria, serum cholesterol, triglyceride, blood urea nitrogen (BUN), uric acid, malondialdehyde, superoxide dismutase; urinary BUN and urinary creatinine. As for signaling, quercetin was completely effective in alleviating the cleaved caspase‐3, being only partially effective in suppressing Bax and Bad, restoring Bcl‐2, and rescuing DNA damage.ConclusionThe CKD status cannot to be ameliorated by naringenin, rutin, and catechin. Comparatively, quercetin may be a better therapeutic candidate.
Stress and neuroticism in Spanish nursing students: A two‐wave longitudinal study
Authors: Fornés‐Vives Joana , García‐Banda Gloria , Frías‐Navarro Dolores , Hermoso‐Rodríguez Enrique , Santos‐Abaunza Pilar 
Published: September 2012

AbstractThe aim of this study was to clarify the direction of the stress–neuroticism relationship in a sample of 200 nursing students from three Spanish universities before their entry into the work force using a two‐wave longitudinal design. The Stressful Life Events Scale and NEO‐FFI Neuroticism subscale were administered at the beginning (T1) and end (T2) of nursing studies. Female students reported higher scores in both perceived stress due to life events and neuroticism than males. Older students scored higher in life events stress than younger ones. High neuroticism was associated with a high level of stress. Finally, neuroticism scores rose in the group in which stress increased from T1 to T2. Our findings partially support the stress causation interactionist model of stress in which life events can modify personality traits. © 2012 Wiley Periodicals, Inc. Res Nurs Health 35:589–597, 2012
Interactive performance and focus groups with adolescents: The power of play
Authors: Norris Anne E. , Aroian Karen J. , Warren Stefanie , Wirth Jeff 
Published: September 2012

AbstractConducting focus groups with adolescents can be challenging given their developmental needs, particularly with sensitive topics. These challenges include intense need for peer approval, declining social trust, short attention span, and reliance on concrete operations thinking. In this article, we describe an adaptation of interactive performance as an alternative to traditional focus group method. We used this method in a study of discrimination experienced by Muslims (ages 13–17) and of peer pressure to engage in sexual behavior experienced by Hispanic girls (ages 10–14). Recommendations for use of this method include using an interdisciplinary team, planning for large amounts of disclosure towards the end of the focus group, and considering the fit of this method to the study topic. © 2012 Wiley Periodicals, Inc. Res Nurs Health 35:671–679, 2012
Doxorubicin or daunorubicin given upfront in a therapeutic window are equally effective in children with newly diagnosed acute lymphoblastic leukemia. A randomized comparison in trial CoALL 07‐03
Authors: Escherich Gabriele , Zimmermann Martin , Janka‐Schaub Gritta ,   on behalf of the CoALL study group
Published: September 2012

AbstractBackgroundThe anthracyclines daunorubicin (DNR) and doxorubicin (DOX) are among the most important drugs in the treatment of childhood acute lymphoblastic leukemia, however there are conflicting in vitro data about the comparative efficacy and equivalent doses of both anthracyclines. To address the question of in vivo efficacy of both anthracyclines, patients enrolled in the CoALL 07‐03 trial were randomized to receive one single dose of either doxorubicin 30 mg/m2, daunorubicin 30 mg/m2, or daunorubicin 40 mg/m2 upfront induction therapy.ProcedureChildren with newly diagnosed B‐Precursor ALL or T‐ALL were eligible for the randomized comparison. From the percentage of blasts and the white blood cell count (WBC) the absolute number of leukemic cells per µl peripheral blood (PB) was calculated and the initial value before DOX/DNR infusion equated as 100%. Main target criterion of this study was the leukemic cell decrease from Day 0 to Day 7.ResultsSeven hundred forty three patients were randomized: 247 to the DOX; 252 to the DNR 30 mg/m2; and DNR to the 40 mg/m2 arm. The in vivo response was similar in all three treatment arms with a comparable blast decline in the peripheral blood. The percentages of patients with a clear non‐response (M3 marrow) and moreover, the level of minimal residual disease (MRD) on Day 15 or at the end of induction were similar.ConclusionIn vivo efficacy of a single dose daunorubicin 30 or 40 mg/m2 is similar to that of doxorubicin given in a dose of 30 mg/m2. Pediatr Blood Cancer 2013;60:254–257. © 2012 Wiley Periodicals, Inc.
Cognitive functioning in allogeneic hematopoietic stem cell transplantation recipients and its medical correlates: a prospective multicenter study
Authors: Scherwath Angela , Schirmer Lena , Kruse Margitta , Ernst Gundula , Eder Matthias , Dinkel Andreas , Kunze Sabine , Balck Friedrich , Bornhäuser Martin , Ehninger Gerhard , Dolan Karin , Gramatzki Martin , Kolb Hans‐Jochem , Heußner Pia , Wilhelm Hans , Beelen Dietrich W. , Schulz‐Kindermann Frank , Zander Axel R. , Koch Uwe , Mehnert Anja 
Published: September 2012

AbstractBackgroundOwing to its neurotoxicity, allogeneic hematopoietic stem cell transplantation (HSCT) carries risks for cognitive impairment. In this multicenter study, we prospectively evaluated cognitive functioning and its medical and demographic correlates in patients undergoing allogeneic HSCT.MethodsA total of 102 patients were consecutively assessed prior to (T0), 100 ± 20 days (T1) after, and 12 ± 1 months (T2) after HSCT (61% men, 41% acute myeloid leukemia). A comprehensive neuropsychological test battery was applied to evaluate attention, memory, executive function, and fine motor function, summing up into 14 test scores.ResultsBefore and after HSCT, patients performed below test norms in up to 50% of the test scores. Patients were mostly impaired on word fluency (24%, T0), fine motor function, and verbal delayed recall (19% each, T2). Impairment on ≥1/5 cognitive domains occurred in 47% (T0) and 41% (T2) of the patients. Performance (mean z‐scores) partially improved over time (i.e., visual span forward, verbal learning, and word fluency). However, from baseline to T2, 16% of the patients showed reliable decline on ≥3/14 test scores (reliable change index method). For the majority of neuropsychological subtests, no associations with conditioning intensity, total body irradiation, graft‐versus‐host disease, cyclosporine treatment, and length of hospital stay were found. Age and premorbid intelligence level were consistently associated with cognition.ConclusionsBelow average cognitive performance is common in this patient group. In addition, a subgroup shows reliable cognitive decline after allogeneic HSCT. Healthcare professionals should be aware of these treatment‐related cognitive side effects. Copyright © 2012 John Wiley & Sons, Ltd.
New reference equations to improve interpretation of infant lung function
Authors: Nguyen The Thanh Diem , Hoo Ah‐Fong , Lum Sooky , Wade Angie , Thia Lena P. , Stocks Janet 
Published: September 2012

AbstractRationaleWith increasing use of infant pulmonary function tests (IPFTs) in both clinical and research studies, appropriate interpretation of results is essential.ObjectivesTo investigate the potential bias associated with “normalising” IPF by expressing results as a ratio of body size and to develop reference ranges for tidal breathing parameters, passive respiratory mechanics (compliance [Crs] and resistance [Rrs]) and plethysmographic functional residual capacity (FRCp) for white infants during the first 2 years of life.MethodsIPFTs were measured using the Jaeger BabyBody system and standardized protocols. Reference equations, adjusted for body size, age, and sex where appropriate, were created using multilevel modeling.ResultsThe ratio of lung function to body length changes markedly with growth, thereby precluding its use for any outcome. While the ratio of tidal volume and Crs to body weight remained relatively constant with growth, this was not the case for FRCp. Even in healthy infants, a strong inverse relationship was observed between lung function/body weight and weight z‐score which could distort interpretation of results in growth‐restricted infants with lung disease, such as cystic fibrosis. Reference equations were derived from 153 healthy white infants on 232 test occasions (median age 35.5 weeks [range: 2.6–104.7]). Crown‐heel length was the strongest predictor of IPF.ConclusionsWhen reporting IPF, use of size‐corrected ratios should be discouraged, with interpretation instead based on appropriate reference equations. The current equations are applicable to white infants and young children up to 2 years of age, studied using the same commercially available equipment. The extent to which these equations are applicable to infants and young children of other ethnic backgrounds or who are tested with different equipment needs to be established. Pediatr Pulmonol. 2013; 48:370–380. © 2012 Wiley Periodicals, Inc.