bluuebunny/crossref_metadata_2025_split huggingface dataset:
A high‐performance liquid chromatography method for the serotonin release assay is equivalent to the radioactive method
Authors: Sono‐Koree N. K. , Crist R. A. , Frank E. L. , Rodgers G. M. , Smock K. J. 
Published: November 2015

SummaryIntroductionThe serotonin release assay (SRA) is considered the gold standard laboratory test for heparin‐induced thrombocytopenia (HIT). The historic SRA method uses platelets loaded with radiolabeled serotonin to evaluate platelet activation by HIT immune complexes. However, a nonradioactive method is desirable. We report the performance characteristics of a high‐performance liquid chromatography (HPLC) SRA method.MethodsWe validated the performance characteristics of an HPLC‐SRA method, including correlation with a reference laboratory using the radioactive method. Serotonin released from reagent platelets was quantified by HPLC using fluorescent detection. Results were expressed as % release and classified as positive, negative, or indeterminate based on previously published cutoffs.ResultsSerum samples from 250 subjects with suspected HIT were tested in the HPLC‐SRA and with the radioactive method. Concordant classifications were observed in 230 samples (92%). Sera from 41 healthy individuals tested negative. Between‐run imprecision studies showed standard deviation of <6 (% release) for positive, weak positive, and negative serum pools. Stability studies demonstrated stability after two freeze–thaw cycles or up to a week of refrigeration.ConclusionThe HPLC‐SRA has robust performance characteristics, equivalent to the historic radioactive method, but avoids the complexities of working with radioactivity.
Predictive or not predictive: understanding the mixed messages from the patient's DNA sequence
Authors: Janssens A Cecile J W , Patch Christine , Skirton Heather 
Published: November 2015

Aims and objectivesThe aim of this discussion paper is to enable nurses to understand how deoxyribonucleic acid analysis can be predictive for some diseases and not predictive for others. This will facilitate nurses to interpret genomic test results and explain them to patients.BackgroundAdvances in technology mean that genetic testing is now commonly performed by sequencing the majority of an individual's genome or exome. This results in a huge amount of data, some of which can be used to predict or diagnose disease.DesignThis is a discussion paper.MethodsThis paper emerged from multiple discussions between the three authors over many months, culminating in a writing workshop to prepare this text.ResultsThe results of DNA analysis can be used to diagnose or predict rare diseases that are caused by a mutation in a single gene. However, while there are a number of genetic factors that contribute to common diseases, the ability to predict whether an individual will develop that condition is limited by the overall heritability of the condition. Environmental factors (such as lifestyle) are likely to be more useful in predicting common disease than genomic testing. Genomic tests may be of use to inform management of diseases in specific situations.ConclusionsGenomic testing will be of use in diagnosing disorders due to single gene mutations, but the use of genomic testing to predict the chance of a patient being affected in the future by a common disease is unlikely to be a realistic option within a health service setting.Relevance to clinical practiceNurses will increasingly be involved in the use of genomic tests in mainstream patient care. However, they need to understand and be able to explain to patients the practical applications of and limitations of such tests.
Rheological and Textural Properties of Sodium Reduced Salt Soluble Myofibrillar Protein Gels Containing Sodium Tri‐Polyphosphate
Authors: Çarkcioğlu E. , Rosenthal A.J. , Candoğan K. 
Published: November 2015

AbstractThe effect of partial replacement of NaCl (50%) with KCl in the presence of sodium tri‐polyphosphate (STPP) on the cooking loss (CL), water‐holding capacity (WHC), rheological and textural properties of salt soluble myofibrillar proteins (SSMP) gels was investigated. KCl substitution, either alone or in combination with STPP, was found worse than NaCl alone in terms of elasticity of the gels (G′). Both KCl and STPP reduced the CL of the gels. While the gels with replacement of NaCl with KCl had lower (P < 0.05) WHC, addition of STPP to this gels gave rise to similar (P > 0.05) WHC to only NaCl added gels. Substitution of NaCl with KCl resulted in a decrease in hardness of gels whereas STPP addition improved the hardness. Results obtained from the present study suggest that substitution 50% of NaCl with KCl in presence of STPP would be a sound salt reduction alternative in meat systems.Practical ApplicationsSalt is one of the most crucial ingredients affecting meat products functionality. However, recent research has focused on reducing salt in meat products due to its association with some health problems such as hypertension. Reducing sodium chloride (NaCl) decreases extractability of salt soluble myofibrillar proteins (SSMP), thus, negatively affects functional properties of the meat systems. One of the approaches in reducing salt is to replace NaCl with other chloride salts, the most common of which is potassium chloride (KCl). This study focused on determining the effect of partially substituting of NaCl with KCl in the presence of sodium tri‐polyphosphate (STPP) on some functional characteristics of meat SSMP gels. Results indicated that 50% NaCl reduction using KCl and STPP could be achieved with an improvement of textural and functional properties in meat gels. Future research should focus on combined effects of salt substitutes on the rheological and textural characteristics of meat gel systems.
Pharmacokinetics of single‐dose oral ponazuril in weanling goats
Authors: Love D. , Gibbons P. , Fajt V. , Jones M. 
Published: November 2015

Ponazuril (toltrazuril sulfone) is a triazine antiprotozoal agent that targets apicomplexan organisms. Ponazuril may have clinical application in the treatment of clinical coccidiosis due to Eimeria species in goats, along with other protozoal infections. To evaluate the absorption, distribution and elimination characteristics of ponazuril in goats, a sensitive, validated high‐pressure liquid chromatography and mass spectroscopy method for ponazuril in caprine plasma was developed. After a single oral dose of ponazuril at 10 mg/kg, plasma samples from seven weanling goats were collected and assayed. Plasma concentrations of ponazuril in the goats peaked at 36 ± 13 h post drug administration at a concentration of 9 ± 2 μg/mL. Concentrations declined to an average of 4.2 ± 0.8 μg/mL after 168 h with an average elimination half‐life of 129 ± 72 h post drug administration. This study shows that ponazuril is relatively well absorbed after a single oral dose in goats. Efficacy trials are underway to determine clinical efficacy of ponazuril in the treatment of clinical coccidiosis in goats at 10 mg/kg dosage.
Longitudinal Study on the Association Between Cardiorespiratory Fitness, Anthropometric Parameters and Blood Lipids
Authors: Mertens Evelien , Clarys Peter , Lefevre Johan , Charlier Ruben , Knaeps Sara , Deforche Benedicte 
Published: November 2015

Background:Longitudinal evidence concerning the association between cardiorespiratory fitness (CRF) and blood lipids and between anthropometric parameters (ANTP) and blood lipids is limited. This study aimed to investigate the association between changes in CRF and ANTP and changes in blood lipids.Methods:In 2002–2004 and 2012–2014, 652 participants were tested. CRF was measured as VO2peak using a maximal ergometer test. Waist circumference (WC) and Body Mass Index (BMI) were used as ANTP. Blood samples were analyzed for total cholesterol (TC), HDL cholesterol, LDL cholesterol and triglycerides. A linear regression analysis was performed to investigate associations between changes in CRF and ANTP and changes in blood lipids.Results:After adjustment a decrease in CRF was associated with an increase in triglycerides and a decrease in HDL cholesterol in men. An increase in WC was associated with an increase in TC, LDL cholesterol and ratio total/HDL cholesterol and a decrease in HDL cholesterol, while an increase in BMI was associated with an increase in ratio total/HDL cholesterol and a decrease in HDL cholesterol.Conclusions:WC and BMI were more longitudinally associated with blood lipids compared with CRF. Improving ANTP can enhance the blood lipid profile, while CRF had only limited influence.
Complete Genome Sequence of Bartonella ancashensis Strain 20.00, Isolated from the Blood of a Patient with Verruga Peruana
Authors: Hang Jun , Mullins Kristin E. , Clifford Robert J. , Onmus-Leone Fatma , Yang Yu , Jiang Ju , Leguia Mariana , Kasper Matthew R. , Maguiña Ciro , Lesho Emil P. , Jarman Richard G. , Richards Allen L. , Blazes David 
Published: November 2015

ABSTRACT

Here we present the complete genome sequence of
Bartonella ancashensis
strain 20.00, isolated from the blood of a Peruvian patient with verruga peruana, known as Carrion's disease.
Bartonella ancashensis
is a Gram-negative bacillus, phylogenetically most similar to
Bartonella bacilliformis
, the causative agent of Oroya fever and verruga peruana.
Whole-Genome Sequence of “
Candidatus
Profftella armatura” from
Diaphorina citri
in Guangdong, China
Authors: Wu F. , Deng X. , Liang G. , Huang J. , Cen Y. , Chen J. 
Published: November 2015

ABSTRACT

The genome of “
Candidatus
Profftella armatura” strain YCPA from
Diaphorina citri
in Guangdong, China, was sequenced. The strain has a chromosome of 457,565 bp, 24.3% G+C content, 364 predicted open reading frames (ORFs), and 38 RNAs, and a plasmid, pYCPA54, of 5,458 bp with 23.9% G+C content and 5 ORFs.
Efficient Self-Stabilizing Algorithm for Independent Strong Dominating Sets in Arbitrary Graphs
Authors: Neggazi Brahim , Guellati Nabil , Haddad Mohammed , Kheddouci Hamamache 
Published: November 2015

In computer networks area, the minimal dominating sets (MDS) and maximal independent sets (MIS) structures are very useful for creating virtual network overlays. Often, these set structures are used for designing efficient protocols in wireless sensor and ad-hoc networks. In this paper, we give a particular interest to one kind of these sets, called Independent Strong Dominating Set (ISD-set). In addition to its domination and independence properties, the ISD-set considers also node’s degrees that make it very useful in practical applications where nodes with larger degrees play important role in the networks. For example, some network clustering protocols chose nodes with large degrees to be cluster-heads, which is exactly the result obtained by an ISD-set algorithm. Thence, we propose the first distributed self-stabilizing algorithm for computing an ISD-set of an arbitrary graph (called ISDS). Then, we prove that ISDS algorithm operates under the unfair distributed scheduler and converges after at most [Formula: see text] rounds requiring only [Formula: see text] space memory per node where Δ is the maximum node degree. The complexity of ISDS algorithm in rounds has the same order as the best known self-stabilizing algorithms for finding MDS and MIS. Moreover, performed simulations and comparisons with well-known self-stabilizing algorithms for MDS and MIS problems showed the efficiency of ISDS, especially for reducing the cardinality of dominating sets founded by the algorithms.
Finanzas y Salud Mental
Authors: Lopez Fernando 
Published: November 2015

Los esfuerzos para cuantificar y mitigar los daños asociados a eventos inesperados que producen perjuicios económicos, como desastres naturales o caídas inesperadas en el valor de los activos, se concentran en los daños materiales. Sin embargo, esta aproximación ignora un tipo de daño altamente relevante para las personas afectadas y la sociedad: el daño a la salud mental. Este artículo discute la medida en que los servicios financieros podrían contribuir a mitigar el efecto de shocks adversos en la salud mental, presenta una selección de la evidencia científica disponible y describe los desafíos que enfrentan los estudios orientados a analizar esta hipótesis.
DISCUTINDO CITY INFORMATION MODELING (CIM) E CONCEITOS CORRELATOS
Authors: Amorim Arivaldo Leão de 
Published: November 2015

<p>Este artigo discute o paradigma <em>City Information Modeling</em> (CIM) a partir de uma abordagem ampla, considerando um viés teórico e conceitual deste e de outros termos relacionados. O CIM tem sido apontado por alguns autores como uma extensão do conceito de BIM para o espaço urbano. Contudo, face às características da cidade em relação à edificação isolada<em> </em>e à profusão de conceitos ora em uso, algumas questões precisam ser aprofundadas. Estes conceitos estão intimamente relacionados, apresentando algum tipo de sobreposição, e talvez por isto, sejam confundidos. Assim, pretende-se ampliar a discussão sobre esses conceitos, contribuindo para a utilização dos mesmos e avançando no estabelecimento de uma conceituação e terminologias que facilitem o desenvolvimento das aplicações e a adoção das tecnologias nas práticas relativas ao planejamento, à gestão e ao monitoramento da cidade. Não e tem a pretensão de esgotar o tema, seja pela vastidão das questões envolvidas, seja ainda pelo reduzido espaço a para discussão das mesmas, mas principalmente, porque estas questões estão “em aberto”, sendo objeto de discussões e controvérsias. </p>
Integrative Mouse and Human Studies Implicate
ANGPT1
and
ZBTB7C
as Susceptibility Genes to Ischemic Injury
Authors: Du Rose , Zhou Jing , Lorenzano Svetlana , Liu Wenming , Charoenvimolphan Nareerat , Qian Baogang , Xu Jun , Wang Jian , Zhang Xinmu , Wang Xin , Berndt Annerose , Devan William J. , Valant Valerie J. , Wang Jinyi , Furie Karen L. , Rosand Jonathan , Rost Natalia , Friedlander Robert M. , Paigen Beverly , Weiss Scott T. 
Published: November 2015

Background and Purpose—
The extent of ischemic injury in response to cerebral ischemia is known to be affected by native vasculature. However, the nonvascular and dynamic vascular responses and their genetic basis are not well understood.

Methods—
We performed a genome-wide association study in 235 mice from 33 inbred strains using the middle cerebral artery occlusion model. Population structure and genetic relatedness were accounted for using the efficient mixed-model association method. Human orthologs to the genes associated with the significant and suggestive single-nucleotide polymorphisms from the mouse strain survey were examined in patients with M1 occlusions admitted with signs and symptoms of acute ischemic stroke.

Results—

We identified 4 genome-wide significant and suggestive single-nucleotide polymorphisms to be associated with infarct volume in mice (rs3694965,
P
=2.17×10
–7
; rs31924033,
P
=5.61×10
–6
; rs32249495,
P
=2.08×10
–7
; and rs3677406,
P
=9.56×10
–6
). rs32249495, which corresponds to angiopoietin-1 (
ANGPT1
), was also significant in the recessive model in humans, whereas rs1944577, which corresponds to
ZBTB7C
, was nominally significant in both the additive and dominant genetic models in humans.
ZBTB7C
was shown to be upregulated in endothelial cells using both in vitro and in vivo models of ischemia.

Conclusions—

Genetic variations of
ANGPT1
and
ZBTB7C
are associated with increased infarct size in both mice and humans.
ZBTB7C
may modulate the ischemic response via neuronal apoptosis and dynamic collateralization and, in addition to
ANGPT1
, may serve as potential novel targets for treatments of cerebral ischemia.
The full-length cDNA cloning and expression profiles of 14-3-3 genes from the mud crab Scylla paramamosain Estampador, 1949
Authors: Shu Miao-An , Long Chen , Dong Wei-Ren , Zhang Pan , Xu Bin-Peng , Guo Xiao-Ling 
Published: November 2015

Two full-length cDNA sequences of14-3-3genes were isolated from the mud crab,Scylla paramamosainEstampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both14-3-3genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp14-3-3genes show 96 and 97% identity, respectively, and both Sp14-3-3cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp14-3-3genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp14-3-3genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp14-3-3genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the14-3-3family in both crustaceans and (in)vertebrates as a whole.
Does the NMME Capture a Recent Decadal Shift toward Increasing Drought Occurrence in the Southwestern United States?
Authors: Barnston Anthony G. , Lyon Bradfield 
Published: November 2015

Abstract
A global-scale decadal climate shift, beginning in 1998/99 and enduring through 2013, has been documented in recent studies, with associated precipitation shifts in key regions throughout the world. These precipitation shifts are most easily detected during March–May when ENSO effects are weak. Analyses have linked this climate shift to a shift in the Pacific decadal variability (PDV) pattern to its negative phase. Here the authors evaluate the predictive skill of the North American Multimodel Ensemble (NMME), and the CFSv2 model alone, in maintaining the observed precipitation shifts in seasonal forecasts, emphasizing the southwestern United States where deficient precipitation has tended to prevail since the late 1990s.
The NMME hindcasts out to 6 months lead are found to maintain the observed decadal precipitation shifts in key locations qualitatively correctly, but with increasingly underestimated amplitude with increasing lead time. This finding holds in the separate CFSv2 model hindcasts. The decadal precipitation shift is relatively well reproduced in the southwestern United States. The general underestimation of the precipitation shift is suggested to be related to a muted reproduction of the observed shift in Pacific sea surface temperature (SST). This conclusion is supported by runs from a different (but overlapping) set of atmospheric models, which when forced with observed SST reproduce the decadal shifts quite well. Overall, the capability of the NMME model hindcasts to reflect the observed decadal rainfall pattern shift, but with weakened amplitude (especially at longer leads), underscores the broader challenge of retaining decadal signals in predictions of droughts and pluvials at seasonal-to-interannual time scales.
The target orientation of the multivariate analysis using algebraic model of constructive logic
Authors: Fedorov S. , Kitanina K. , Khromushin V. , Khromushin O. 
Published: November 2015

Multivariate analysis, including algebraic model of constructive logic, is often used in medical practice and biological research. To carry out such studies, it is necessary a array of source information (analyzed cases) and purpose, which is most often selected one of the values of the factors. At the same time, in the practice of analytical calculations there are cases when the target value cannot be set explicitly. The aim of this work is to provide a method of calculating target values for specific cases of morbidity and mortality.
The proposed method is based on counting the number of instances of each value of each factor and their share in the total number of cases. The product of the assessed values of each involved factor, compared with the set of the threshold value, determines a value corresponding to the achievement of the goal. To confirm the proposed method on the array of 208269 deaths, the authors built a mathematical model using algebraic model of constructive logic. Evaluation of a mathematical model confirmed the performance of the proposed method of calculating the target value, since the simulation results are most consistent with known estimates obtained by other methods.
A prospective study of a modified pin-in-plaster technique for treatment of fractures of the distal radius
Authors: Mirghasemi S. A. , Rashidinia S. , Sadeghi M. S. , Talebizadeh M. , Rahimi N. 
Published: November 2015

ObjectivesThere are various pin-in-plaster methods for treating fractures of the distal radius. The purpose of this study is to introduce a modified technique of ‘pin in plaster’.MethodsFifty-four patients with fractures of the distal radius were followed for one year post-operatively. Patients were excluded if they had type B fractures according to AO classification, multiple injuries or pathological fractures, and were treated more than seven days after injury. Range of movement and functional results were evaluated at three and six months and one and two years post-operatively. Radiographic parameters including radial inclination, tilt, and height, were measured pre- and post-operatively.ResultsThe average radial tilt was 10.6° of volar flexion and radial height was 10.2 mm at the sixth month post-operatively. Three cases of pin tract infection were recorded, all of which were treated successfully with oral antibiotics. There were no cases of pin loosening. A total of 73 patients underwent surgery, and three cases of radial nerve irritation were recorded at the time of cast removal. All radial nerve palsies resolved at the six-month follow-up. There were no cases of median nerve compression or carpal tunnel syndrome, and no cases of tendon injury.ConclusionOur modified technique is effective to restore anatomic congruity and maintain reduction in fractures of the distal radius. Cite this article: Bone Joint Res 2015;4:176–180