bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of Vitis GRF4-GIF1 improves regeneration efficiency in diploid Fragaria vesca Hawaii 4
Authors: Sanchez Esther Rosales , Price R. Jordan , Marangelli Federico , McLeary Kirsty , Harrison Richard J. , Kundu Anindya 
Published: July 2024

Abstract

Background
Plant breeding played a very important role in transforming strawberries from being a niche crop with a small geographical footprint into an economically important crop grown across the planet. But even modern marker assisted breeding takes a considerable amount of time, over multiple plant generations, to produce a plant with desirable traits. As a quicker alternative, plants with desirable traits can be raised through tissue culture by doing precise genetic manipulations. Overexpression of morphogenic regulators previously known for meristem development provides an efficient strategy for easier regeneration and transformation in multiple crops. In this study, we show the results for overexpression of chimeric GRF4-GIF1 in diploid strawberry Fragaria vesca Hawaii 4 (strawberry) where Vitis GRF4-GIF1 chimera provides significantly higher regeneration efficiency.
Results
We present here a comprehensive protocol for strawberry regeneration and transformation under control condition as compared to ectopic expression of GRF4-GIF1 chimeras from different plants. We report that ectopic expression of Vitis vinifera VvGRF4-GIF1 provide significantly higher regeneration efficiency during retransformation over wild-type plants. On the other hand, deregulated expression of miRNA resistant version of Vitis GRF4-GIF1 or TaGRF4-GIF (wheat) resulted in abnormalities. Transcriptomic analysis between the different chimeric GRF4-GIF1 lines indicate that differential expression of FvExpansin might be responsible for the pleiotropic effects. Similarly, cytokinin dehydrogenase/oxygenase and cytokinin responsive response regulators also showed differential expression indicating GRF4-GIF1 pathway playing important role in controlling cytokinin homeostasis.
Conclusion
Our data indicate that ectopic expression of Vitis vinifera VvGRF4-GIF1 chimera can provide significant advantage over wild-type plants during strawberry regeneration without producing any pleiotropic effects seen for the miRNA resistant VvGRF4-GIF1.
Carbon Sequestration Strategies in the Oil and Gas Industry: Focus on Reservoir Storage
Authors: Kumar B. Santhosh , Venkat GS. Kapilesh , JAYAKUMAR C 
Published: July 2024

Abstract

Carbon sequestration is of crucial importance in combating the negative effects of greenhouse gas emissions and is becoming increasingly important in the current environmental debate. This study examines different methods of carbon sequestration, particularly in the reservoirs of major oil and gas industries. Important factors to consider in this complicated area are the identification of suitable storage sites, thorough evaluation of injection methods, implementation of strong monitoring techniques, and comprehensive assessment of potential environmental impacts. Through a thorough analysis of current research and real-world examples, this study provides valuable perspectives on the effectiveness and practicality of carbon storage in reservoirs developed specifically for the oil and gas sector.
Age-related changes in the prevalence of negative responses to the online body shaming among teenagers aged 11-17
Authors: Kvintova Jana , Novotny Jan Sebastian , Vachova Lucie , Szotkowski René , Liu Hongyang , Kopecky Kamil 
Published: July 2024

Abstract

The increased time spent online and the growth of social networking among teenagers are associated with a heightened risk of online body shaming, eliciting diverse negative responses. Understanding how these responses vary with age remains an area requiring further exploration. This research investigates age-related variations in the prevalence of reactions to online body shaming among teenagers aged 11–17. Findings indicate distinct trends in the prevalence of responses across different age groups, with early teenagers (11–13 years) commonly experiencing somatic difficulties, intense negative emotions, and a propensity to withdraw from social interactions. Conversely, an escalation in drug use, diminished trust, and a rise in negative thoughts and uncontrollable emotions are noted among middle to late teenagers (14–17 years). Additionally, the occurrence of negative responses is markedly higher in girls than in boys. These insights underscore the importance of tailoring prevention programs and interventions to address the specific needs and experiences of teenagers at various stages of adolescence. The study provides significant implications for future research and practitioners working with youth to mitigate the adverse effects of online body shaming.
Malaria Outbreak Investigation And Associated Risk Factors In Siraro District, Oromia Region, Ethiopia,2021
Authors: Mideksa Girma 
Published: July 2024

Abstract

Background
In Ethiopia, malaria is highly seasonal in many communities ,with epidemic-prone transmission patterns and serious public health emergencies that have a major impact on health and the economy. This investigation aimed to assess the malaria outbreaks, determine risk factors for infection and preventive measures in the Siraro district.
Methods
A unmatched case-control study with a 1:1 case-to-control ratio was conducted in the Siraro District from 16 June 2021 to 03 August 2021. The calculated sample size for this study was 148 and a simple random sampling technique was used to select cases and controls. We reviewed the previous five year’s malaria data to establish a threshold level. Both descriptive and analytical statistical procedures were performed. The data were entered using Epi-Info version 7.2.4.0 software and analyzed by using SPSS version 25. Multivariable logistic regression analysis was conducted to identify independent factors associated with malaria infection.
Results
Of the total malaria cases (n = 183), Plasmodium falciparum species are about 149(81.4%) and almost 100% of the cases were confirmed by RDT/Microscopy. Age group ≥ 5 years were the most affected with an attack rate (AR) of 2.04/1000. Multivariate analysis revealed the place of stay during night adjusted Odds Ratio (AOR) 5.62, 95% CI = 1.016–31.069, a person with malaria in the house with AOR 15.74,95% CI = 3.029–81.865, presence of stagnant water near to house AOR of 25.60, 95% CI = 4.696-139.649, availability of plants with temporary water pools in the compound an AOR of 90.94,95% CI = 9.019-917.012 and a person who does not hear about malaria prevention on insecticide-treated bed nets utilization adjusted Odds Ratios (AOR) of 0.14,95% CI: 0.076, 1.061 were the main associated risk factors for the occurrence of the outbreak.
Conclusions
The malaria outbreak in Siraro District mostly affected children above 5 years of age. Of the variables studied, a people with malaria in the house, stagnant water, and plants with temporary water pools and lack of awareness about malaria transmission were all significantly associated with the occurrence of malaria outbreaks. The community should be aware of malaria prevention and control measures.
Interaction of Genetics Risk Score (GRS) and Plant-Based Diet on factors Atherogenic and body adiposity indexes among overweight and obese women: a cross-sectional study
Authors: Hajmir Mahya Mehri , Mirzababaei Atieh , Abaj Faezeh , Aali Yasaman , Samadi Mahsa , Mirzaei Khadijeh 
Published: July 2024

Abstract

Background:
The association between plant-based foods with obesity, cardiovascular diseases (CVD), and their novel predictive biomarkers considering genetic predisposition remains uncertain. Given that diet is a significant and modifiable risk factor, we sought to investigate the interactions between plant-based diet and genetic susceptibility with atherogenic factors, and visceral and body adiposity indices in Iranian women.
Methods:
This cross-sectional study was conducted on 377 obese and overweight women, aged 18–48 from Iran. Using standard protocols, anthropometric indices, body composition, physical activity, and serum profiles were measured. A validated 147-item semi-quantitative food frequency questionnaire (FFQ) was used to create three plant-based diets including the overall plant-based diet index (PDI), healthy plant-based diet index (hPDI), and unhealthy plant-based diet index (uPDI). A genetic risk score (GRS) was calculated based on the risk alleles of the three BMI-related SNPs. The interaction between GRS and PDI was analyzed using a generalized linear model (GLM).
Result
There was a negative significant interaction between tertile 2 of PDI and moderate risk allele and high-risk alleles with AIP, TGy, LAP, and VAI compared to the low-risk allele (P-value < 0.05). There was a negative borderline significant interaction between tertile 2 of hPDI and moderate risk allele (P-value = 0.05) with ABSI compared to low-risk allele participants. There was a positive significant interaction between tertile 2 of uPDI and moderate risk allele with CRI.I (P-value = 0.03), CRI.II (P-value = 0.03) compared to low-risk allele participants. Also, there was a positive significant interaction between tertile 3 of uPDI and high-risk allele with ABSI (P-value = 0.02) compared to low-risk allele participants.
Conclusion
The present study provides evidence that interaction between PDI, hPDI, and uPDI with GSR is associated with atherogenic index and body composition. Prospective and interventional studies in different populations and ethnicities need to be conducted to further the knowledge about examining the interaction between PDI, hPDI, and uPDI with GSR are associated with atherogenic index and body composition.
Calibrated CAR Signaling Enables Low-Dose Therapy in Large B-Cell Lymphoma
Authors: Park Jae , Palomba M.Lia , Perica Karlo , Devlin Sean , Shah Gunjan , Dahi Parastoo , Lin Richard , Salles Gilles , Scordo Michael , Nath Karthik , Valtis Yannis , Lynch Alec , Cathcart Elizabeth , Zhang Honglei , Schöder Heiko , Leithner Doris , Liotta Kelly , Yu Alina , Stocker Kelsey , Li Jia , Dey Agnish , Sellner Leopold , Singh Reshma , Sundaresan Varsha , Zhao Faye , Mansilla-Soto Jorge , He Changhao , Meyerson Joel , Hosszu Kinga , McAvoy Devin , Wang Xiuyan , Riviere Isabelle , Sadelain Michel 
Published: July 2024

Abstract

We designed a CD19-targeted CAR comprising a calibrated signaling module, termed 1XX, that differs from that of conventional CD28/CD3z and 4-1BB/CD3z CARs. Here we report the first-in-human, phase 1 clinical trial of 19(T2)28z-1XX CAR T cells in relapsed/refractory large B-cell lymphoma. We hypothesized that 1XX CAR T cells may be effective at low doses and investigated 4 doubling dose levels starting from 25x106 CAR T cells. The overall response rate (ORR) was 82% and complete response (CR) rate 71% in the entire cohort (n=28) and 88% ORR and 75% CR in 16 patients treated at 25x106. With the median follow‐up of 24 months, the 1-year EFS was 61% (95% CI: 45-82%). Overall, grade ≥3 CRS and ICANS rates were low at 4% and 7%. The calibrated potency of the 1XX CAR affords excellent efficacy at low cell doses and may benefit the treatment of other hematological malignancies, solid tumors and autoimmunity.
Identification of prognostic gene markers for the early diagnosis of colorectal cancer
Authors: sharma kirti 
Published: July 2024

Abstract

Background:
Colorectal Cancer (CRC) is the frequently occurring malignant tumor in colon and rectum with high mortality rate. The signaling pathway involved in CRC and CRC driven genes are largely unknown.
Methods
To identify the gene signatures which help in early diagnosis of CRC, we downloaded three datasets (GSE24514, GSE8671 and GSE21510) from the Gene Expression Omnibus (GEO) Database. GO and KEGG pathway enrichment analysis were conducted using DAVID database. A protein–protein interaction (PPI) network was constructed using STRING and cytoscape software. These hub genes were verified by survival analysis using GEPIA database.
Results
A total of 120 DEGs were identified including (75 upregulated genes and 45 downregulated genes). Seven modules were identified from protein –protein interaction network using MCODE plug in tool of cytoscape, only three Modules (1, 2 and 3) selected with score ≥ 5 and node ≥ 10. Module 1 contained downregulated genes and Module 2 and 3 contained upregulated genes. Hub genes identified from Module 1 with connectivity score ≥ 16 included CDK1, CCNB1, FOXM1, RRM2, MAD2L1, NEK2, MCM4 and PBK. Out of 8 genes examined, only 3 exhibited significant correlations with overall survival among CRC patients (p > 0.05). MAD2L1, MCM4, and PBK demonstrated relatively lower expression levels of these genes were correlated with poor prognosis in CRC patients. Hub genes from Modules 2 and 3 (connectivity score ≥ 6) included MYL9, CNN1, MYH11, MYLK, TAGLN, GUCA2A, GUCA2B, ZG16 and SLC26A3. Survival analysis indicated that higher expression of MYL9, CNN1 and TAGLN correlated with poor prognosis, while lower expression of ZG16 and SLC26A3 was linked to poorer outcomes in CRC patients (p < 0.05). These eight hub genes, believed to promote tumor activity, are promising candidates for new CRC therapeutic targets.
Conclusion
Eight hub DEGs (MAD2L1, MCM4, PBK, MYL9, CNN1, TAGLN, ZG16 and SLC26A3) were identified, to be strongly correlated with the overall survival of patients with CRC based on GEO and GEPIA data. These eight genes have the potential as novel and independent prognostic biomarkers for early diagnosis of CRC and forecasting clinical results of CRC patients. Several studies revealed that suppression of these genes inhibits the proliferation of CRC.
Bibliometric Analysis on Recent Topics in ILS Research
Authors: Rosser Pablo , Soler  Seila 
Published: July 2024

Abstract

This article presents a bibliometric analysis of recent topics in ILS (Individual Learning Styles) research. The methodology employed was based on a systematic review of documents and scientific publications related to the study object from 2004 to the present utilizing the Scopus database and the 'bibliometrix' package in the R programming language. Several decision steps were followed to ensure the reliability and validity of the analysis. The findings allowed for the identification of the most cited journals, the most prominent authors, and the most common affiliations among the authors of the analyzed documents. Additionally, different models of learning style preferences and their impact on the teaching process were explored. The findings contribute significantly to understanding the dynamics of research in the studied discipline. Regarding the most cited journals, "Computers & Education" was found to be the leading journal in terms of the total number of citations, followed by "Educational Research Review" and "Learning and Instruction". As for the most prominent authors, several relevant names were identified, such as David Kolb, Richard Felder, and Peter Honey. Regarding the most common affiliations among the authors, it was found that several Spanish universities lead this list. On the other hand, this study explored different learning style models and their impact on the educational process. It was found that there is a wide variety of theoretical models on learning style, but there is no clear consensus on which model is best to apply in an educational context. However, the importance of considering students' learning styles to adapt the educational process to their individual needs was highlighted. In conclusion, this bibliometric analysis provides an overview of the current state of ILS research and can be useful for researchers, educators, and professionals interested in this field. The obtained results can also be used to identify current trends in ILS research and to guide future investigations in this area.
Geospatial Analysis Applied to Epidemiological Studies of Rabies Disease: A Systematic Review
Authors: Cruz Zito Viegas da , Adnyana I Made Dwi Mertha , de Souza Jeronimo 
Published: July 2024

Abstract

Rabies is a deadly zoonotic disease that remains a global health problem. The incidence of this disease is increasing, but it has not yet been overcome by various parties. The easy transmission of this disease from animals to humans necessitates the use of epidemiological analysis methods to accelerate its eradication and control. This systematic review aimed to assess the types of spatial methods used in rabies epidemiological studies published between January 2014 and April 2024. Thirty-eight studies were selected, and 28 different spatial methods were used in rabies studies during that period, with two methods being the most frequently used. Few articles have applied spatial analysis methods in rabies studies; however, whenever they were applied, they contributed to a better understanding of the geospatial diffusion of rabies. This review highlights the importance of geospatial analysis for understanding the spread of rabies, identifying hotspots, and identifying the need for more targeted and effective interventions.
An Incomplete Picture: A Scoping Review of Global, Original Qualitative Rare Disease Research
Authors: Gush Marianne , Moosa Shahida 
Published: July 2024

Abstract

Background
Rare diseases, globally and collectively, pose a significant social and economic burden with far reaching implications in both high and middle- to low-income countries. An in-depth understanding of the first-hand experiences of rare disease patients, caregivers and service providers is required to fully understand the challenges these individuals and groups face. Only once such an understanding has been developed, can steps be taken to collaborate towards effectively addressing the relevant issues.
Results
The current scoping review searched the most prominent databases in order to isolate original, qualitative, rare disease research published between 2004 and 2024 to determine the scope and nature of existing qualitative rare disease research. The analysis of the 150 identified studies shows a substantial overrepresentation of research from both Europe (53%) and North America (29%), and a severe underrepresentation of qualitative rare disease research from Africa (1%).
Conclusions
The lack of qualitative rare disease research from Africa represents a lack of understanding, not only of the lived experiences of Africans, but also an ignorance of the complex cultural factors which would potentially affect the experience of “commonly” identified themes, such as interactions with healthcare professionals and parent advocacy, stigma and social support.
Мутации в генах KRAS/NRAS и эффективность индукционной терапии по трехкомпонентным схемам на основе бортезомиба у пациентов с впервые диагностированной множественной миеломой
Authors: Сергеева Анна Михайловна , Сурин В. Л. , Чабаева Ю. А. , Крайзман А. А. , Соловьев М. В. , Куликов С. М. , Менделеева Л. П. 
Published: July 2024

AIM. To identify the KRAS and NRAS gene mutations in patients with newly diagnosed multiple myeloma (ММ) and to classify them according to the depth of antitumor response to bortezomib-based triplet induction therapy.
MATERIALS & METHODS. The trial enrolled 89 patients with newly diagnosed MM prior to chemotherapy. Among them, there were 45 women and 44 men aged 30–82 years (median 58.5 years). ММ was diagnosed according to IMWG criteria (2014). Bone marrow (BM) plasma cells were isolated from the aspirate using gradient method with subsequent immunomagnetic CD138 marker selection. The KRAS and NRAS gene mutations in BM CD138+ cells were identified with Sanger sequencing method. The proteomic programs MutationTaster, Polyphen2, and FATHMM-XF were used for mutation analysis in the KRAS and NRAS genes. All patients received bortezomib-based triplet chemotherapy as first-line treatment. The response depth was assessed after completing 6 cycles of PAD and VCD regimens. Antitumor response was evaluated according to IMWG (2016) criteria.
RESULTS. The mutation rate in the gene family RAS was 42 % (37/89). The analysis focused on the data from 33 patients with mutations detected and response identified after 6 cycles of treatment. In 22 out of 33 patients, deep response was not achieved, whereas 11 patients showed complete remission (CR) + very good partial remission (VGPR). In the group of patients without mutations in the gene family RAS, the response to therapy meeting the CR + VGPR criteria was 64 % (27/42). The differences appeared to be significant (p = 0.008). The clinical data and the evaluation of primary treatment outcomes provided the basis for distinguishing a group of 9 prognostically unfavorable mutations: NRAS Gly13Asp, Gln61His; KRAS Gly12Ala, Gly12Asp, Gly12Val, Gly13Asp, Gln61Arg, Gln61His, and Ala146Val.
CONCLUSION. The mutations in KRAS and NRAS belonging to the gene family RAS had a negative effect on the efficacy of the bortezomib-based triplet induction therapy. Mutation variants in the RAS family genes differed in prognostic significance. The analysis results helped to identify the mutation variants associated with the worse response to therapy: NRAS Gly13Asp, Gln61His; KRAS Gly12Ala, Gly12Asp, Gly12Val, Gly13Asp, Gln61Arg, Gln61His, and Ala146Val.
Chezy's resistance coefficient in vaulted rectangular conduit
Authors: Loukam Imed , Achour Bachir 
Published: July 2024

ABSTRACT

Calculating resistance coefficients for flows in pipes and channels, such as the Darcy–Weisbach coefficient of friction and the Chezy or Manning coefficients, is a complex process aimed at accurately reflecting flow under load or a free surface. Generally, these coefficients are given as constants, although they can be challenging to ascertain due to implicit models expressing these coefficients. Therefore, the design of pipes and channels necessitates an explicit and comprehensible expression of the resistance coefficient, incorporating numerous flow parameters, including aspect ratio, kinematic viscosity, pipe slope, and roughness of internal walls. To achieve this outcome, we suggest employing the rough model method (RMM) giving the volume flowrate to identify the Chezy's resistance coefficient C in uniform and free surface flow for the vaulted rectangular conduit. This applies in both cases where the diameter of the geometric profile of the conduit is known or unknown.