bluuebunny/crossref_metadata_2025_split huggingface dataset:
The DEAD-box helicase Ded1 from yeast is an mRNP cap-associated protein that shuttles between the cytoplasm and nucleus
Authors: Senissar Meriem , Saux Agnès Le , Belgareh-Touzé Naïma , Adam Céline , Banroques Josette , Tanner N. Kyle 
Published: July 2014

AbstractThe DEAD-box helicase Ded1 is an essential yeast protein that is closely related to mammalian DDX3 and to other DEAD-box proteins involved in developmental and cell cycle regulation. Ded1 is considered to be a translation-initiation factor that helps the 40S ribosome scan the mRNA from the 5′ 7-methylguanosine cap to the AUG start codon. We used IgG pull-down experiments, mass spectrometry analyses, genetic experiments, sucrose gradients, in situ localizations and enzymatic assays to show that Ded1 is a cap-associated protein that actively shuttles between the cytoplasm and the nucleus. NanoLC-MS/MS analyses of purified complexes show that Ded1 is present in both nuclear and cytoplasmic mRNPs. Ded1 physically interacts with purified components of the nuclear CBC and the cytoplasmic eIF4F complexes, and its enzymatic activity is stimulated by these factors. In addition, we show that Ded1 is genetically linked to these factors. Ded1 comigrates with these proteins on sucrose gradients, but treatment with rapamycin does not appreciably alter the distribution of Ded1; thus, most of the Ded1 is in stable mRNP complexes. We conclude that Ded1 is an mRNP cofactor of the cap complex that may function to remodel the different mRNPs and thereby regulate the expression of the mRNAs.
Efficacy of Fecal Microbiota Transplantation in 2 Children With Recurrent Clostridium difficile Infection and Its Impact on Their Growth and Gut Microbiome
Authors: Walia Ritu , Garg Shashank , Song Yang , Girotra Mohit , Cuffari Carmen , Fricke Wolfgang Florian , Dutta Sudhir K. 
Published: July 2014

ABSTRACTFecal microbiota transplantation (FMT) is recognized as an alternative therapeutic modality for recurrent Clostridium difficile infection (RCDI); however, data on its efficacy in children are lacking, including its effect on their growth and fecal microbiota. We report on 2 young children (<3 years old) who failed available therapeutics for RCDI, but responded remarkably well to FMT. Besides resolution of clinical features of C difficile infection (CDI), FMT administration led to marked improvement in their growth, along with increased microbiota diversity, especially proportion of Bacteroides. Our 2 cases illustrate the efficacy of FMT in children with RCDI and its positive effect on their growth and gut microbiota.
Extracting robust trends in species’ distributions from unstructured opportunistic data: a comparison of methods
Authors: Isaac Nick J.B. , van Strien Arco J. , August Tom A. , de Zeeuw Marnix P. , Roy David B. 
Published: July 2014

AbstractPolicy-makers increasingly demand robust measures of biodiversity change over short time periods. Long-term monitoring schemes provide high-quality data, often on an annual basis, but are taxonomically and geographically restricted. By contrast, opportunistic biological records are relatively unstructured but vast in quantity. Recently, these data have been applied to increasingly elaborate science and policy questions, using a range of methods. At present we lack a firm understanding of which methods, if any, are capable of delivering unbiased trend estimates on policy-relevant timescales.We identified a set of candidate methods that employ either selection criteria or correction factors to deal with variation in recorder activity. We designed a computer simulation to compare the statistical properties of these methods under a suite of realistic data collection scenarios. We measured the Type 1 error rates of each method-scenario combination, as well as the power to detect genuine trends.We found that simple methods produce biased trend estimates, and/or had low power. Most methods are robust to variation in sampling effort, but biases in spatial coverage, sampling effort per visit, and detectability, as well as turnover in community composition all induced some methods to fail. No method was robust to all forms of variation in recorder activity.We warn against the use of simple methods. We identify three methods with complementary strengths and weaknesses that are useful for estimating timely trends. Sophisticated correction factor methods, including Occupancy and Frescalo, offer the greatest potential in the long-term. Methods based solely on selection criteria are inherently limited, but a combination or ensemble of approaches may be required to generate trends that are both robust and powerful. Small amounts of information about sampling intensity, captured at the point of data collection, would greatly enhance the utility of opportunistic data and make future trend estimates more reliable.
Stable heteroplasmy at the single cell level is facilitated by inter-cellular exchange of mtDNA
Authors: Jayaprakash Anitha D , Benson Erica , Gone Swapna , Liang Raymond , Shim Jaehee , Lambertini Luca , Toloue Masoud M , Wigler Mike , Aaronson Stuart A , Sachidanandam Ravi 
Published: July 2014

AbstractEukaryotic cells carry two genomes, nuclear (nDNA) and mitochondrial (mtDNA), which are ostensibly decoupled in their replication, segregation and inheritance. It is increasingly appreciated that heteroplasmy, the occurrence of multiple mtDNA haplotypes in a cell, plays an important biological role, but its features are not well understood. Accurately determining the diversity of mtDNA has been difficult, due to the relatively small amount of mtDNA in each cell (< 1% of the total DNA), the intercellular variability of mtDNA content and mtDNA pseudogenes (Numts) in nDNA. To understand the nature of heteroplasmy, we developed Mseek, a novel technique to purify and sequence mtDNA. Mseek yields high purity (> 90%) mtDNA and its ability to detect rare variants is limited only by sequencing depth, providing unprecedented sensitivity and specificity. Using Mseek, we confirmed the ubiquity of heteroplasmy by analyzing mtDNA from a diverse set of cell lines and human samples. Applying Mseek to colonies derived from single cells, we find heteroplasmy is stably maintained in individual daughter cells over multiple cell divisions. We hypothesized that the stability of heteroplasmy could be facilitated by inter-cellular exchange of mtDNA. We explicitly demonstrate this exchange by co-culturing cell lines with distinct mtDNA haplotypes. Our results shed new light on the maintenance of heteroplasmy and provide a novel platform to investigate features of heteroplasmy in normal and diseased states.
Strengthening reliability in high hazard industries: reconciling tensions for impact
Authors: H. Offstein Evan , Kniphuisen Raymond , Robin Bichy D. , Stephen Childers Jr J. 
Published: July 2014

Purpose
– Recent lapses in the management of high hazard organizations, such as the Fukushima event or the Deepwater Horizon blast, add considerable urgency to better understand the complicated and complex phenomena of leading and managing high reliability organizations (HRO). The purpose of this paper is to offer both theoretical and practical insight to further strengthen reliability in high hazard organizations.

Design/methodology/approach
– Phenomenological study based on over three years of research and thousands of hours of study in HROs conducted through a scholar-practitioner partnership.

Findings
– The findings indicate that the identification and the management of competing tensions arising from misalignment within and between public policy, organizational strategy, communication, decision-making, organizational learning, and leadership is the critical factor in explaining improved reliability and safety of HROs.

Research limitations/implications
– Stops short of full-blown grounded theory. Steps were made to ensure validity; however, generalizability may be limited due to sample.

Practical implications
– Provides insight into reliably operating organizations that are crucial to society where errors would cause significant damage or loss.

Originality/value
– Extends high reliability research by investigating more fully the competing tensions present in these complex, societally crucial organizations.
E-service quality: a study of online shoppers in India
Authors: Kandulapati Suresh , Shekhar Bellamkonda Raja 
Published: July 2014

Purpose
– The purpose of this paper is to measure the service quality of online shops using E-S-QUAL and validating the tool in an Indian context. Also, the study examines the mediating role of service value on the relationship between service quality and customer satisfaction in the context of online services.

Design/methodology/approach
– An online survey is conducted by administering a structured questionnaire to online shoppers in India. The dimensions of E-S-QUAL (Parasuraman et al., 2005) are adopted without revision to measure electronic service quality (e-SQ) of online shops. Structural equation modeling, reliability tests, analysis of variance (one-way), correlation and regression analysis are performed through SPSS and AMOS.

Findings
– The study reveals that E-S-QUAL as developed by Parasuraman et al. (2005) is valid for measuring e-SQ in the context of Indian online shopping. The study proves that there is a significant impact of perceived e-SQ on perceived service value. Further, perceived service value mediates the relationship between e-SQ and customer satisfaction.

Originality/value
– The study helps the online service providers/e-commerce industry to understand e-shoppers’ perception and e-SQ in India. Also, it can contribute to formulating the best e-marketing strategies by which to tap into the e-shopping market in India.
A study on health care consumers’ diabetes term usage across identified categories
Authors: Zhang Jin , Zhao Yiming , Dimitroff Alexandra 
Published: July 2014

Purpose– The purpose of this paper is to investigate health care consumers’ diabetes term usage patterns based on Yahoo!Answers social question and answers (Q&A) forum, identified characteristics and relationships among terms within three pairs of related categories identified from the Q&A log, and revealed users’ diabetes term usage patterns.Design/methodology/approach– The Q&A analysis method allowed first-hand investigation of massive data from health care consumers. Visual term clustering analysis across categories was conducted using a multi-dimensional scaling (MDS) visualization method which provides an intuitive and interactive way to explore and discover term association patterns in a visual environment. Closely related categories were identified and corresponding visual term clustering analyses between categories (Sign & Symptom and Organ & Body Part; Diagnosis and Test; and Diagnosis and Medication) as well at the term level were analyzed.Findings– The findings show that there are close relationships between terms in two related categories. Related terms were grouped and patterns were revealed. All the stress values of the MDS analyses fall below 0.10 and RSQ for each of the combined categories is over 0.90 which indicate the investigated terms were well clustered in the visual analyses.Originality/value– The study provides a unique research methodology for similar consumer health research studies. The results of this study offer insight into consumer health term use behavior, and enrich existing thesauri and subject heading lists, enhance diabetes-related web sites or portals, and improve effectiveness of internal search engines.
Health and safety coordinators in building projects
Authors: Almén Lena , J. Larsson Tore 
Published: July 2014

Purpose
– In order to reduce the number of injuries on construction sites, a European Directive prescribes that the clients shall appoint safety and health coordinators in their projects. The purpose of this paper is to find out who are appointed to be health and safety coordinators for the design and planning phase and what they do in order to prevent injuries on sites.

Design/methodology/approach
– Since the 1st of January 2009, there shall be a coordinator for the design and planning phase in Swedish construction projects. Telephone interviews were made with the coordinators in 42 Swedish building projects.

Findings
– The coordinators’ education and experiences varied widely, as well as their descriptions of their duties: no duties, administration and active injury prevention. The coordinators who were classified as most active had at least one additional leading role in the projects.

Research limitations/implications
– The study is qualitative for an increased understanding, not a statistical reflection of the coordinator population.

Practical implications
– The legislation needs to clarify whether the early conceptual phase of the project is included in the coordinator's commission and whether she/he is supposed to participate in identifying, assessing and reducing risks through design changes. These clarifications will have an influence on when the coordinator should be appointed and what competence she/he needs.

Originality/value
– The study increases the understanding of how the health and safety coordinators of the planning and projecting phase of building projects perceive their mission and what factors, according to them, have an influence on what they do.
Could refurbishment of “traditional” buildings reduce carbon emissions?
Authors: Atkins Richard , Emmanuel Rohinton 
Published: July 2014

Purpose
– Evaluate the post occupancy performance of a typical “traditional” building using multiple post occupancy evaluation (PoE) protocols against design intents to learn lessons about their suitability in meeting UK's climate change reduction targets. The paper aims to discuss these issues.

Design/methodology/approach
– PoE studies of a single case study, Norton Park, using three PoE methodologies. Gaps and overlaps between the PoE protocols are assessed and their role in improving energy and carbon emission performance of traditional buildings is explored.

Findings
– Refurbishment of the type undertaken in this case study could halve the energy use in traditional buildings with comparable savings in CO2 emission.

Research limitations/implications
– Traditional buildings could positively contribute to achieving climate change reduction targets; regular feedback loops improve performance over time.

Practical implications
– Quantification of the likely national benefit of focusing retrofit actions on traditional buildings is explored.

Originality/value
– The research study demonstrates that very high levels of energy saving can be achieved when traditional buildings are refurbished. In addition on-going monitoring and PoE studies highlight opportunities to optimise the performance of traditional buildings.
Determining the cost of poor quality and its impact on productivity and profitability
Authors: Mahmood Shahid , M. Ahmed Syed , Panthi Kamalesh , Ishaque Kureshi Nadeem 
Published: July 2014

Purpose
– The purpose of this paper is to examine how effectively the cost appraisal system proposed measures the cost of poor quality (COPQ) in a construction project. The paper first formulates how COPQ can be measured and later clarifies the relationship between COPQ, labor productivity, and profitability.

Design/methodology/approach
– In order to measure COPQ, the researchers prepared data entry forms for recording COPQ items on a daily basis and formulated the cost contribution of lost material, lost man-hours, lost machinery hours, and lost overhead on the overall COPQ for the project. The proposed method was then applied in a case study.

Findings
– The results showed that, for the 60-days study period, COPQ decreased by about 24 percent while labor productivity and profitability increased by about 17 and 11 percent, respectively, after the implementation of COPQ measuring system. This study further supports the use of the COPQ system in construction projects as a mechanism to facilitate continuous improvement.

Originality/value
– COPQ is a major cost that is often ignored in construction projects due to the difficulty of measuring it. This paper presents a COPQ measuring and recording system capable of identifying COPQ. The implementation of the system is shown to increase productivity and profitability as demonstrated by the project used for the case study.
Constraints in implementing Public Private Partnership (PPP) in Malaysia
Authors: Ismail Suhaiza , Azzahra Haris Fatimah 
Published: July 2014

Purpose
– The purpose of this paper is to study two objectives. First, it intends to scrutinize the challenges in implementing Public Private Partnership (PPP) by examining the factors that hinder the successful adoption of PPP in Malaysia. Second, it aims to investigate the differences in the perceptions of the government and the private sector pertaining to the hindrance factors.

Design/methodology/approach
– A questionnaire survey was used to elicit the perceptions of the public and private sectors concerning the constraints of PPP implementation in Malaysia. A total of 122 usable responses were obtained, which were analysed using Statistical Package for Social Sciences software. The mean score and mean score ranking were used to examine the importance of the hindrance factors based on the overall responses as well as on the respective responses of the public and the private sectors. An independent sample t-test was used to examine the differences in the perceptions between the two sectors.

Findings
– The overall results show that “lengthy delays in negotiation”, “lack of government guidelines and procedures on PPP”, “higher charge to direct users”, “lengthy delays because of political debate” and “confusion over government objectives and evaluation criteria” are the top five constraints for adopting PPP in Malaysia. In terms of the differences in the perceptions between the public and private sector groups, the statistical test results indicate that there are only significant differences in the perceptions for two hindrance factors.

Originality/value
– The paper contributes to the existing literature on PPP, which is currently limited, by focusing on the challenges of implementing PPP in a developing country (i.e. Malaysia). More importantly, this paper provides evidence concerning the differences in the opinion of two key players (i.e. the government and the private sector) on the PPP hindrance factors.
Shopping orientation as a stable consumer disposition and its influence on consumers’ evaluations of retailer communication
Authors: B. Büttner Oliver , Florack Arnd , S. Göritz Anja 
Published: July 2014

Purpose
– The present aims to examine whether interindividual differences in consumers’ shopping orientations reflect a stable consumer disposition (i.e. chronic shopping orientation; CSO). Furthermore, it examines whether this disposition influences consumers’ evaluations of retailer communication. Consumers may shop under an experiential or a task-focused shopping orientation.

Design/methodology/approach
– This research builds on four studies; three were conducted online and one was conducted in the laboratory. Study 1 applied a longitudinal design, Studies 2 and 3 applied a cross-sectional design and Study 4 applied an experimental design.

Findings
– Study 1 shows that CSO is stable over time. Study 2 finds that interindividual differences in CSO are stable across different retail domains. Studies 3 and 4 demonstrate that experiential shoppers prefer stimulation-oriented claims, whereas task-focused shoppers prefer efficiency-oriented claims.

Originality/value
– The value of shopping orientation for customer segmentation and tailored marketing largely depends on whether interindividual differences in CSO are stable. The present research is the first to demonstrate that CSO, indeed, exists as a stable consumer disposition. In addition, the research demonstrates that shopping orientation moderates the evaluation of retailer communication. Overall, the results demonstrate that CSO is a valuable construct for customer segmentation and tailored communication in retailing.
Looking beyond impulse buying
Authors: Sharma Piyush , Sivakumaran Bharadhwaj , Marshall Roger 
Published: July 2014

Purpose
– This paper aims to conceptualize consumer impulsiveness (CI) as a global trait to explore its influence on a wider range of consumer behaviours and also presents a revised CI scale. Prior research on CI focuses on the impulse buying context and does not establish the cross-cultural invariance of the CI scale.

Design/methodology/approach
– Two studies with undergraduate and MBA students in Singapore, UK and USA were used to develop the revised CI scale and to test its cross-cultural measurement invariance and predictive validity.

Findings
– CI is a three-dimensional construct with cognitive (imprudence), affective (self-indulgence) and behavioural (lack of self-control) dimensions. However, self-indulgence and lack of self-control positively (do not) correlate for consumers with independent (interdependent) self-concepts. These three dimensions also vary in their influence on different types of self-regulatory failures.

Research limitations/implications
– The student participants used in all the studies may be relatively younger and better educated compared to average consumers. Hence, there is a need to test the revised CI scale with diverse consumer populations.

Practical implications
– The revised CI scale would help future researchers study the influence of CI across diverse cultures and self-regulatory failures in a reliable and rigorous manner.

Social implications
– Our findings may help control the onset and spread of self-regulatory failures among young consumers by early identification of their psychological origins.

Originality/value
– This paper extends the scope of CI beyond impulse buying to study its impact on self-regulatory failure across five diverse behavioural domains (driving, eating, entertainment, shopping and substance abuse).
Exploring the fit of e-books to the needs of medical academics in Australia
Authors: Shimizu Wilson Concepción , D’Ambra John , Drummond Robyn 
Published: July 2014

Purpose
– The purpose of this study is to explore the extent to which e-books fit the needs of medical academics of the University of New South Wales (UNSW) in the performance of their academic tasks.

Design/methodology/approach
– A web-based survey was distributed to all UNSW academics in medicine, and 224 completed responses were analyzed according to the attributes of a task-technology fit (TTF) model developed for e-books in academic settings.

Findings
– Although the UNSW Library had access to > 225,000 e-books, usage by medical academics was relatively low (38 per cent); however, most (92 per cent) predicted that they would be using e-books within the next five years. Nearly two-thirds (65 per cent) had portable devices including smartphones, and 90 per cent rated the ability to search across full text in an e-book of moderate-to-high importance. Research tasks dominated the use of e-books, and 71 per cent agreed that e-books helped improve their overall productivity.

Research limitation/implications
– Only 224 (8 per cent) of 2,790 medical academics at UNSW participated in the study. The low response rate and over-representation of research only academics limit the extent of generalization of the findings.

Originality/value
– This is the first study on the use of e-books among academics in the Faculty of Medicine – comprising nearly 64 per cent of all UNSW academic staff. The findings highlight the extent of e-books used by medical academics and their enthusiasm for access to digital resources. There is also the suggestion that the library must continue to develop services to ensure delivery of task-compatible e-books to medical academics in increasingly mobile environments.