bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole transcriptome analyses of six thoroughbred horses before and after exercise using RNA-Seq
Authors: Park Kyung-Do , Park Jongsun , Ko Junsu , Kim Byung Chul , Kim Heui-Soo , Ahn Kung , Do Kyoung-Tag , Choi Hansol , Kim Hak-Min , Song Sanghoon , Lee Sunghoon , Jho Sungwoong , Kong Hong-Sik , Yang Young Mok , Jhun Byung-Hak , Kim Chulhong , Kim Tae-Hyung , Hwang Seungwoo , Bhak Jong , Lee Hak-Kyo , Cho Byung-Wook 
Published: September 2012

Abstract

Background
Thoroughbred horses are the most expensive domestic animals, and their running ability and knowledge about their muscle-related diseases are important in animal genetics. While the horse reference genome is available, there has been no large-scale functional annotation of the genome using expressed genes derived from transcriptomes.

Results
We present a large-scale analysis of whole transcriptome data. We sequenced the whole mRNA from the blood and muscle tissues of six thoroughbred horses before and after exercise. By comparing current genome annotations, we identified 32,361 unigene clusters spanning 51.83 Mb that contained 11,933 (36.87%) annotated genes. More than 60% (20,428) of the unigene clusters did not match any current equine gene model. We also identified 189,973 single nucleotide variations (SNVs) from the sequences aligned against the horse reference genome. Most SNVs (171,558 SNVs; 90.31%) were novel when compared with over 1.1 million equine SNPs from two SNP databases. Using differential expression analysis, we further identified a number of exercise-regulated genes: 62 up-regulated and 80 down-regulated genes in the blood, and 878 up-regulated and 285 down-regulated genes in the muscle. Six of 28 previously-known exercise-related genes were over-expressed in the muscle after exercise. Among the differentially expressed genes, there were 91 transcription factor-encoding genes, which included 56 functionally unknown transcription factor candidates that are probably associated with an early regulatory exercise mechanism. In addition, we found interesting RNA expression patterns where different alternative splicing forms of the same gene showed reversed expressions before and after exercising.

Conclusion
The first sequencing-based horse transcriptome data, extensive analyses results, deferentially expressed genes before and after exercise, and candidate genes that are related to the exercise are provided in this study.
Central poststroke pain: somatosensory abnormalities and the presence of associated myofascial pain syndrome
Authors: de Oliveira Rogério Adas Ayres , de Andrade Daniel Ciampi , Machado André Guelman Gomes , Teixeira Manoel Jacobsen 
Published: September 2012

Abstract

Background
Central post-stroke pain (CPSP) is a neuropathic pain syndrome associated with somatosensory abnormalities due to central nervous system lesion following a cerebrovascular insult. Post-stroke pain (PSP) refers to a broader range of clinical conditions leading to pain after stroke, but not restricted to CPSP, including other types of pain such as myofascial pain syndrome (MPS), painful shoulder, lumbar and dorsal pain, complex regional pain syndrome, and spasticity-related pain. Despite its recognition as part of the general PSP diagnostic possibilities, the prevalence of MPS has never been characterized in patients with CPSP patients. We performed a cross-sectional standardized clinical and radiological evaluation of patients with definite CPSP in order to assess the presence of other non-neuropathic pain syndromes, and in particular, the role of myofascial pain syndrome in these patients.

Methods
CPSP patients underwent a standardized sensory and motor neurological evaluation, and were classified according to stroke mechanism, neurological deficits, presence and profile of MPS. The Visual Analogic Scale (VAS), McGill Pain Questionnaire (MPQ), and Beck Depression Scale (BDS) were filled out by all participants.

Results
Forty CPSP patients were included. Thirty-six (90.0%) had one single ischemic stroke. Pain presented during the first three months after stroke in 75.0%. Median pain intensity was 10 (5 to 10). There was no difference in pain intensity among the different lesion site groups. Neuropathic pain was continuous-ongoing in 34 (85.0%) patients and intermittent in the remainder. Burning was the most common descriptor (70%). Main aggravating factors were contact to cold (62.5%). Thermo-sensory abnormalities were universal. MPS was diagnosed in 27 (67.5%) patients and was more common in the supratentorial extra-thalamic group (P <0.001). No significant differences were observed among the different stroke location groups and pain questionnaires and scales scores. Importantly, CPSP patients with and without MPS did not differ in pain intensity (VAS), MPQ or BDS scores.

Conclusions
The presence of MPS is not an exception after stroke and may present in association with CPSP as a common comorbid condition. Further studies are necessary to clarify the role of MPS in CPSP.
A tool to facilitate clinical biomarker studies - a tissue dictionary based on the Human Protein Atlas
Authors: Kampf Caroline , Bergman Julia , Oksvold Per , Asplund Anna , Navani Sanjay , Wiking Mikaela , Lundberg Emma , Uhlén Mathias , Ponten Fredrik 
Published: September 2012

Abstract
The complexity of tissue and the alterations that distinguish normal from cancer remain a challenge for translating results from tumor biological studies into clinical medicine. This has generated an unmet need to exploit the findings from studies based on cell lines and model organisms to develop, validate and clinically apply novel diagnostic, prognostic and treatment predictive markers. As one step to meet this challenge, the Human Protein Atlas project has been set up to produce antibodies towards human protein targets corresponding to all human protein coding genes and to map protein expression in normal human tissues, cancer and cells. Here, we present a dictionary based on microscopy images created as an amendment to the Human Protein Atlas. The aim of the dictionary is to facilitate the interpretation and use of the image-based data available in the Human Protein Atlas, but also to serve as a tool for training and understanding tissue histology, pathology and cell biology. The dictionary contains three main parts, normal tissues, cancer tissues and cells, and is based on high-resolution images at different magnifications of full tissue sections stained with H & E. The cell atlas is centered on immunofluorescence and confocal microscopy images, using different color channels to highlight the organelle structure of a cell. Here, we explain how this dictionary can be used as a tool to aid clinicians and scientists in understanding the use of tissue histology and cancer pathology in diagnostics and biomarker studies.
Altered regulatory mechanisms governing cell survival in children affected with clustering of autoimmune disorders
Authors: Palamaro Loredana , Giardino Giuliana , Santamaria Francesca , Ramenghi Ugo , Dianzani Umberto , Pignata Claudio 
Published: September 2012

Abstract
Clustering of Autoimmune Diseases (CAD) is now emerging as a novel clinical entity within monogenic immune defects with a high familial occurrence. Aim of this study is to evaluate the regulatory mechanisms governing cell survival, paying a particular attention to Fas-induced apoptosis, in a cohort of 23 children affected with CAD. In 14 patients, Fas stimulation failed to induce cell apoptosis and in 1 case it was associated with Fas gene mutation. Our study highlights the importance to evaluate cell apoptosis in the group of children with CAD, which, with this regard, represents a distinct clinical entity.
ELF and super-diversity: a case study of ELF multilingual practices from a business context
Authors: Cogo, Alessia 
Published: September 2012

Abstract
This article explores the link between English as a Lingua Franca (ELF) and super-diversity in the multilingual business context of a small IT company, where English is used as a lingua franca and various linguistic resources play an important role in the company practices. The aim of the study is to examine the practices, orientations to and use of ELF and multilingual resources within an ethnographically-oriented approach, with data collected through observations, interviews, focus groups and recordings of naturally-occurring interactions. The findings show that the company's practices are highly multilingual, whereby ‘languaging’ is a common and positively valued phenomenon. Results also show that ELF is highly collaborative, both in spoken and written communication, and the staff's sociolinguistic repertoire is sensitive to the interlocutors' communicative resources.
ELF in international supervision: a nexus analysis approach
Authors: Soukup, Barbara , Kordon, Kathrin 
Published: September 2012

Abstract
In the literature on professional international consulting, the use of a foreign language (such as English) is typically cast as problematic and as an obstacle to successful intervention. In this article, we present evidence to the contrary, showing that conducting a specific form of professional consulting called ‘supervision’ in English as a Lingua Franca (ELF) can be beneficial rather than detrimental to the outcome. We analyze data of one international supervision event using the ethnographic framework of ‘nexus analysis’ (Scollon and Scollon 2004). Nexus analysis operates by investigating how macro-level societal discourses (e.g. professional discourses of supervision) and cultural tools (e.g. the use of ELF) interplay in and mediate local moments of interaction. In our case, using ELF in international supervision can slow down the process, which in turn is actually an important intervention strategy in supervision because it facilitates reflection work and is thus conducive to positive outcomes. We argue that nexus analysis is well poised to investigate the effects of such interplay of ELF use and societal (professional) discourses on the ground. Further, conducting studies that show the benefits of ELF use in supervision can ultimately engender more positive attitudes among professionals in this regard.
Radiolarian assemblages of Middle and Late Jurassic to early Late Cretaceous (Cenomanian) ages from an olistolith record pelagic deposition within the Bornova Flysch Zone in western Turkey
Authors: Tekin Ugur Kagan , Göncüoglu M. Cemal , Uzuncimen Seda 
Published: September 2012

Abstract
The Bornova Flysch Zone (BFZ) in NW Anatolia comprises several olistoliths or tectonic slivers, representing various parts of the Izmir-Ankara ocean. Radiolarian assemblages extracted from one of the olistoliths of the BFZ, cropping out along the Sögütlü section, to the NE Manisa city, were studied in detail. The lowermost part of the section contains latest Bajocian – early Callovian radiolarian taxa, followed by radiolarian assemblages indicating Late Jurassic to early Late Cretaceous (Cenomanian) ages. Previous studies reveal that the Izmir-Ankara oceanic basin was initially opened during late Ladinian – early Carnian. The new radiolarian data obtained from this olistolith reveals that relatively condensed, and possibly more or less continuous, pelagic sedimentation took place during the late Middle Jurassic to early Late Cretaceous in a non-volcanic oceanic basin closer to the Tauride-Anatolide platform margin.
Assessment of role of rosmarinic acid in preventing oxidative process of low density lipoproteins
Authors: Tache Andreia , Radu Gabriel-Lucian , Litescu Simona-Carmen 
Published: September 2012

AbstractThis study aimed to assess the antioxidant compound effects on oxidisable substrates, using an effective bio-mimetic system based on human low density lipoproteins (LDL). Thermally generated radicals induce LDL oxidative changes to be identified and quantified. The bio-mimetic system thus developed responded linearly to radicals’ concentration over a range of 10−6-10−5 mol L−1. Cu2+ accentuates lipoperoxidation but, when rosmarinic acid was present, Cu2+ produced an unexpected effect, i.e. increased antioxidant efficiency against lipoperoxidation. Rosmarinic acid inhibits production of lipoperoxides by up to 30 % in the absence of Cu2+ and up to 70 % in its presence when the rosmarinic acid-to-Cu molar ratio is 1: 1.
Glaucoma suspect & Humphrey Field Analyzer a correlation
Authors: Dahal P , Rizal AP , Karki DB 
Published: September 2012

Glaucoma originally meant "clouded", in Greek.The term glaucoma refers to a group of diseases that have in common characteristic optic neuropathy with associated visual field loss for which elevated intraocular pressure is one of the primary risk factor. The purpose of the study is to correlate the clinically diagnosed cases of glaucoma suspect with the Humphrey Field Analyzer (HFA). Fifty cases of glaucoma suspect who attended the glaucoma clinic of Nepal Eye Hospital Tripureswor, Kathmandu, Nepal and who meets at least two criteria, among the four types of glaucoma suspects were advised for the HFA for the study. In this study out of 50 patient, 36 (72%) patients had normal visual field. 14 (28%) patients had thinning of the neural retinal rim (NRR) in both eyes. The significant relation with thinning of neural retina rim and glaucomatous hemifield test was found in the study. Journal of College of Medical Sciences-Nepal,2012,Vol-8,No-1, 23-28 DOI: http://dx.doi.org/10.3126/jcmsn.v8i1.6822
A Comparative Pharmacoeconomic Assessment of Two Surfactants for the Prevention of Respiratory Distress Syndrome
Authors: Gdovin Joette M. , Moya Fernando , Vishalpura Tejal , Grogg Amy 
Published: September 2012

OBJECTIVE The use of exogenous surfactants among preterm infants for the prevention and treatment of respiratory distress syndrome (RDS) has led to economic and cost-effectiveness evaluations of these products. Lucinactant (Surfaxin), a novel, peptide-based, synthetic surfactant, has been shown to significantly reduce RDS-related mortality, compared with the most commonly prescribed animal-derived surfactant, beractant (Survanta). Infants who survive expend significant healthcare resources; therefore, the impact of improved survival through 1-year corrected age was evaluated in a prospectively defined pharmacoeconomic analysis. The objectives of this study were to estimate the healthcare resource utilization, economic impact, and cost-effectiveness of lucinactant versus beractant for the prevention of RDS among surviving very low birth weight (VLBW) preterm infants weighing 600 to 1250 grams.
METHODS A decision-analytic model was developed to compare the healthcare resource utilization, economic impact, and cost-effectiveness of lucinactant versus beractant.
RESULTS Infants who received lucinactant had fewer neonatal intensive care unit (NICU) days and fewer NICU days on mechanical ventilation compared with infants who received beractant. Total healthcare costs for the initial stay in the NICU were lower by $8,803 among infants who received lucinactant compared with infants who received beractant. The incremental cost per life saved was $40,309 for lucinactant compared with beractant.
CONCLUSIONS Administration of lucinactant to surviving VLBW preterm infants resulted in fewer NICU days and fewer NICU days on mechanical ventilation compared with beractant. Fewer NICU days translates into lower total costs among infants who received lucinactant. This comprehensive pharmacoeconomic analysis indicates that lucinactant is a cost-effective therapy for the prevention of RDS among preterm infants.
Evaluation of Conformity of a First Prescription of Lipid-Based Formulation of Amphotericin B in a University-Teaching Pediatric Hospital
Authors: Agogué Claire , Bussières Jean-François , Dehaut Catherine , Lebel Denis , Brochet Marie-Sophie 
Published: September 2012

OBJECTIVE Invasive fungal infections are an important cause of morbidity and mortality in immunodeficient children. Amphotericin B is an important therapeutic agent for the treatment of invasive fungal infections but is associated with significant toxicities and high acquisition costs. The purpose of this study was to evaluate physician adherence to a local guideline for the use of lipid-based amphotericin B.
METHODS The study was approved through Pharmacology & Therapeutics (P&T) committee activities. A retrospective drug utilization review (DUR) was conducted. All orders written between January 1, 2003, and December 31, 2004, were reviewed. Demographic and descriptive clinical data were collected as well as variables related to the drug order process. Conformity rates were calculated for the primary objective criteria (authorized prescribers – infectious disease members; recommended drug of choice—Abelcet; accepted indications; and presence of underlying conditions).
RESULTS A total of 109 orders for 70 patients were reviewed by a single research assistant for a 2-year period. Global conformity rate for all four criteria was calculated at 7.3%. Non-conformity was mostly associated with the absence of underlying conditions (e.g., prerenal insufficiency or presence of nephrotoxicity due to amphotericin B desoxycholate) in 84.5% of the cases. Infusionrelated adverse drug reactions partly explained a switch to a non-formulary lipid-based amphotericin B product. External factors (newly published results since the adoption of the guideline and continuous marketing practices) and internal factors (availability of non-formulary process, inefficient DUR process) could have contributed to non-adherence to a local guideline.
CONCLUSION This study shows low adherence to P&T committee drug guidelines on lipid-based amphotericin B. Continuous and efficient DUR processes should be in place to monitor drug guideline adherence.
A Cost Minimization Comparison of Two Surfactants—Beractant and Poractant alfa—Based Upon Prospectively Designed, Comparative Clinical Trial Data
Authors: Marsh Wallace , Smeeding James , York John M. , Ramanathan Rangasamy , Sekar Krishnamurthy 
Published: September 2012

OBJECTIVES To compare the pharmacoeconomic profiles of beractant (Survanta®, Ross Laboratories, Columbus, Ohio) and poractant alfa (Curosurf®, DEY LP, Napa, CA) via a cost-minimization analysis.
METHODS This analysis was based upon clinical data from two previously published studies (Speer C, et al. Arch Dis Child 1995;72: F8-13; and Ramanathan R, et al. Am J Perinatol 2004; 21:109-19) where investigators found significant differences in the number of doses required to achieve a similar clinical response. Our analyses employed several models based upon single-use or multiple-use of single-use vial scenarios, average wholesale pricing, and costs computed on a per-patient basis. Model 1 involved single-dose vials and mean weight of the infants (both trials). Models 2 and 3, based on individual patient weights, assessed single-dose and multiple-use of single-dose vials cost scenarios, respectively. Individual patient weights allowed for statistical evaluation in Models 2 and 3.
RESULTS Model 1 savings with poractant alfa treatment was $949.67 (53%) based upon Speer and $617.90 (46%) based upon Ramanathan. Models 2 and 3 reported savings for poractant alfa of $220.50 (20%) (P = 0.11) and $180 (20%) (P = 0.018), respectively over beractant.
CONCLUSIONS These analyses would suggest poractant alfa may offer a less costly, clinically-equivalent option. Savings may vary with vial usage and mix, patient weight distribution, and how surfactants are used in practice. Institutions utilizing surfactants may wish to examine usage patterns, dosing protocols, and patient mix to determine what potential savings may exist.
MDI versus Nebulizers for Acute Asthma
Authors: Raissy Hengameh H. , Kelly H. William 
Published: September 2012

OBJECTIVE: To evaluate studies comparing metered dose inhalers with holding chambers to nebulizers in the emergency department for the treatment of asthma exacerbation.
DATA SOURCE: Primary articles and systematic review provided by the Cochrane Airways Review Group of the Cochrane Library identified by MEDLINE search (1966–February 2004) and through secondary sources.
DATA SYNTHESIS: The Cochrane review included 21 randomized clinical trials conducted in hospital emergency departments comparing clinical outcomes following β2 agonist administration via a nebulizer or a metered dose inhaler with holding chamber. Although the relative risk ratio of hospital admission with metered dose inhaler and holding chamber did not differ in children or in adults compared to the nebulizer delivery, none of the individual studies reviewed were powered to detect a difference in the rate of hospital admission. Specific factors in the treatment of acute asthma such as assessment of severity, appropriate outcome selection, appropriate dose selection, and appropriate delivery systems need to be considered to critically evaluate the literature.
CONCLUSION: Although available randomized clinical trials suggest equivalency of metered dose inhaler plus holding chambers and nebulized delivery of inhaled β2 agonists, these trials are biased to show no difference in response. There is no data to support the advantage of one method over the other in mild to moderate asthmatic patients either clinically or economically.
La compétence de contextualisation au coeur de la situation d’enseignement-apprentissage
Authors: Sauvage Luntadi Laetitia , Tupin Frédéric 
Published: September 2012

La notion de « situation professionnelle », telle que nous proposons de la visiter, suppose de questionner, dans un même mouvement, la place des contextes et le rôle des acteurs au sein des situations d’enseignement-apprentissage. Il s’agit pour nous d’interroger la contextualisation de la démarche d’enseignement engagée au regard des publics accueillis et des conditions d’exercice de la profession d’enseignant. Définir la contextualisation comme « un art de faire » qui relève d’une compétence professionnelle suppose alors de poser la légitimité du/des « contexte(s) » comme vecteur(s) explicatif(s). Le modèle conceptuel développé s’inscrit dans la lignée de la théorie de la structuration d’Anthony Giddens (1987). Il est complété par le modèle écologique du développement humain proposé par Bronfenbrenner (1986).
Isis et le destin en Grèce hellénistique : la circulation d’Isis-Tychè
Authors: Briaud Stéphanie 
Published: September 2012

Nous pouvons dire qu’il existe une Isis égyptienne puis une Isis gréco-romaine, cette dernière se diffusant dans tout le bassin méditerranéen grâce à un réseau de fidèles et de prêtres très investis dans son culte. Pour accroître le succès de leur divinité, cette communauté n’hésite pas, à travers les textes gravés (les arétalogies) et l’iconographie, à la parer d’un « habillage » grec, en développant certains aspects esquissés dans la théologie pharaonique, mais avec une sensibilité hellénistique, susceptible de toucher un plus large public. Tel est le cas des rapports entre Isis et le destin.