bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tartessos
Authors: López‐Ruiz Carolina 
Published: June 2015

Abstract
Tartessos appears in the classical sources as an area in southern Iberia, with the Guadalquivir River (Roman Baetis) as its main axis, roughly comprising Huelva, Cádiz, Seville, and Córdoba in southern Spain and part of the Portuguese Algarve. In modern scholarship, the term is applied to the protohistorical culture that flourished between the eighth to sixth centuries BCE, predating the later Turdetanians. This culture is manifest through archaeological and literary (Greek and Roman) sources and marked by the complex interaction between indigenous groups and Phoenician settlers along the Mediterranean and Atlantic coasts of the Iberian Peninsula. Tartessos is the first widely known urban and literate culture from the far western Mediterranean.
Theory of Hydrogen Migration in Organic–Inorganic Halide Perovskites
Authors: Egger David A. , Kronik Leeor , Rappe Andrew M. 
Published: June 2015

AbstractSolar cells based on organic–inorganic halide perovskites have recently been proven to be remarkably efficient. However, they exhibit hysteresis in their current–voltage curves, and their stability in the presence of water is problematic. Both issues are possibly related to a diffusion of defects in the perovskite material. By using first‐principles calculations based on density functional theory, we study the properties of an important defect in hybrid perovskites—interstitial hydrogen. We show that differently charged defects occupy different crystal sites, which may allow for ionization‐enhanced defect migration following the Bourgoin–Corbett mechanism. Our analysis highlights the structural flexibility of organic–inorganic perovskites: successive iodide displacements, combined with hydrogen bonding, enable proton diffusion with low migration barriers. These findings indicate that hydrogen defects can be mobile and thus highly relevant for the performance of perovskite solar cells.
Ein Redoxhydrogel schützt die O2‐empfindliche [FeFe]‐Hydrogenase aus Chlamydomonas reinhardtii vor oxidativer Zerstörung
Authors: Oughli Alaa Alsheikh , Conzuelo Felipe , Winkler Martin , Happe Thomas , Lubitz Wolfgang , Schuhmann Wolfgang , Rüdiger Olaf , Plumeré Nicolas 
Published: June 2015

AbstractDie Integration empfindlicher Katalysatoren in Redoxpolymere ist eine Möglichkeit, diese vor desaktivierenden Molekülen wie O2 zu schützen. [FeFe]‐Hydrogenasen sind Enzyme, die die Oxidation sowie Produktion von H2 katalysieren. Da sie aber durch O2 irreversibel desaktiviert werden, war die Verwendung dieser Enzyme unter aeroben Bedingungen bisher unmöglich. Die Integration solcher Hydrogenasen in mit Viologenderivaten modifizierten Hydrogelfilmen ermöglicht auch in Gegenwart von O2 katalytische Ströme für die H2‐Oxidation und demonstriert damit einen Schutzmechanismus unabhängig von Reaktivierungsprozessen. Im Hydrogel werden die Elektronen aus der durch die Hydrogenase katalysierten H2‐Oxidation zur Hydrogel‐Elektrolyt‐Grenzfläche transportiert, um dort die schädlichen O2‐Moleküle abzufangen, bevor sie die Hydrogenase desaktivieren können. Wir illustrieren mögliche Anwendungen dieses Schutzmechanismus für eine Biobrennstoffzelle bei gemischter H2/O2‐Zufuhr.
A Redox Hydrogel Protects the O2‐Sensitive [FeFe]‐Hydrogenase from Chlamydomonas reinhardtii from Oxidative Damage
Authors: Oughli Alaa Alsheikh , Conzuelo Felipe , Winkler Martin , Happe Thomas , Lubitz Wolfgang , Schuhmann Wolfgang , Rüdiger Olaf , Plumeré Nicolas 
Published: June 2015

AbstractThe integration of sensitive catalysts in redox matrices opens up the possibility for their protection from deactivating molecules such as O2. [FeFe]‐hydrogenases are enzymes catalyzing H2 oxidation/production which are irreversibly deactivated by O2. Therefore, their use under aerobic conditions has never been achieved. Integration of such hydrogenases in viologen‐modified hydrogel films allows the enzyme to maintain catalytic current for H2 oxidation in the presence of O2, demonstrating a protection mechanism independent of reactivation processes. Within the hydrogel, electrons from the hydrogenase‐catalyzed H2 oxidation are shuttled to the hydrogel–solution interface for O2 reduction. Hence, the harmful O2 molecules do not reach the hydrogenase. We illustrate the potential applications of this protection concept with a biofuel cell under H2/O2 mixed feed.
A Strongly Spin‐Frustrated FeIII7 Complex with a Canted Intermediate Spin Ground State of S=7/2 or 9/2
Authors: Mondal Kartik Chandra , Mereacre Valeriu , Kostakis George E. , Lan Yanhua , Anson Christopher E. , Prisecaru Ion , Waldmann Oliver , Powell Annie K. 
Published: June 2015

AbstractA disk‐shaped [FeIII7(Cl)(MeOH)6(μ3‐O)3(μ‐OMe)6 (PhCO2)6]Cl2 complex with C3 symmetry has been synthesised and characterised. The central tetrahedral FeIII is 0.733 Å above the almost co‐planar FeIII6 wheel, to which it is connected through three μ3‐oxide bridges. For this iron‐oxo core, the magnetic susceptibility analysis proposed a Heisenberg–Dirac–van Vleck (HDvV) mechanism that leads to an intermediate spin ground state of S=7/2 or 9/2. Within either of these ground state manifolds it is reasonable to expect spin frustration effects. The 57Fe Mössbauer (MS) analysis verifies that the central FeIII ion easily aligns its magnetic moment antiparallel to the externally applied field direction, whereas the other six peripheral FeIII ions keep their moments almost perpendicular to the field at stronger fields. This unusual canted spin structure reflects spin frustration. The small linewidths in the magnetic Mössbauer spectra of polycrystalline samples clearly suggest an isotropic exchange mechanism for realisation of this peculiar spin topology.
Probing the Diastereoselectivity of Staudinger Reactions Catalyzed by N‐Heterocyclic Carbenes
Authors: Hans Morgan , Wouters Johan , Demonceau Albert , Delaude Lionel 
Published: June 2015

AbstractThe reaction of ethylphenylketene with 1,3‐dimesitylimidazol‐2‐ylidene (IMes) or 1,3‐dimesitylimidazolin‐2‐ylidene (SIMes) afforded the corresponding azolium enolates in high yields. The two zwitterions were fully characterized by various analytical techniques. Their thermal stabilities were monitored by thermogravimetric analysis and the molecular structure of SIMes⋅EtPhCCO was determined by means of X‐ray crystallography. A mechanism was proposed to account for the trans‐diastereoselectivity observed in the [2+2] cycloaddition of ketenes and N‐protected imines catalyzed by N‐heterocyclic carbenes and an extensive catalytic screening was performed to test its validity. The steric bulk of the NHC catalyst markedly affected the cis/trans ratio of the model β‐lactam product. The nature of the solvent used to carry out the Staudinger reaction also significantly influenced its diastereoselectivity. Conversely, the nature of the substituent on the N‐sulfonated imine reagent and the reaction temperature were less critical parameters.
Controllable Preparation of Polyaniline–Graphene Nanocomposites using Functionalized Graphene for Supercapacitor Electrodes
Authors: Liu Xianbin , Zheng Yuying , Wang Xiaoli 
Published: June 2015

AbstractIn order to explore the effect of graphene surface chemistry on electrochemical performance based on polyaniline–graphene hybrid material electrodes, four different polyaniline–graphene nanocomposites were fabricated with graphene oxide, reduced graphene oxide, aminated graphene and sulfonated graphene as carriers, respectively. The nanocomposites feature various structures and morphologies, which could be used to more deeply understand the morphology and structure effects caused by surface chemistry on electrochemical performance. The experimental results reveal that functionalized electronegative graphene was conducive to the vertical and neat growth of polyaniline (PANI) nanorods. The array architecture endowed the PANI–GS nanocomposite with a large ion‐accessible surface area and high‐efficiency electron‐ and ion‐transport pathways. Meanwhile, the introduction of sulfonic acid functional groups accelerated the redox reaction with doping and dedoping of the PANI. Thereby, the PANI–GS nanocomposite exhibited a high specific capacitance of 863.2 F g−1 at a current density of 0.2 A g−1 and the excellent rate capability of 67.4 % (581.6 F g−1 at 5 A g−1), which were much better than the other three nanocomposites produced.
Comparing Cyclophellitol N‐Alkyl and N‐Acyl Cyclophellitol Aziridines as Activity‐Based Glycosidase Probes
Authors: Jiang Jianbing , Beenakker Thomas J. M. , Kallemeijn Wouter W. , van der Marel Gijsbert A. , van den Elst Hans , Codée Jeroen D. C. , Aerts Johannes M. F. G. , Overkleeft Herman S. 
Published: June 2015

AbstractThe synthesis and evaluation as activity‐based probes (ABPs) of three configurationally distinct, fluorescent N‐alkyl cyclophellitol aziridine isosteres for profiling GH1 β‐glucosidase (GBA), GH27 α‐galactosidase (GLA) and GH29 α‐fucosidase (FUCA) is described. In comparison with the corresponding acyl aziridine ABPs reported previously, the alkyl aziridine ABPs are synthesized easily and are more stable in mild acidic and basic media, and are thus easier to handle. The β‐glucose‐configured alkyl aziridine ABP proves equally effective in labeling GBA as its N‐acyl counterpart, whereas the N‐acyl aziridines targeting GLA and FUCA outperform their N‐alkyl counterparts. Alkyl aziridines can therefore be an attractive alternative in retaining glycosidase ABP design, but in targeting a new retaining glycosidase both N‐alkyl and N‐acyl aziridines are best considered at the onset of a new study.
Length‐Scale‐Dependent Phase Transformation of LiFePO4: An In situ and Operando Study Using Micro‐Raman Spectroscopy and XRD
Authors: Siddique N. A. , Salehi Amir , Wei Zi , Liu Dong , Sajjad Syed D. , Liu Fuqiang 
Published: June 2015

AbstractThe charge and discharge of lithium ion batteries are often accompanied by electrochemically driven phase‐transformation processes. In this work, two in situ and operando methods, that is, micro‐Raman spectroscopy and X‐ray diffraction (XRD), have been combined to study the phase‐transformation process in LiFePO4 at two distinct length scales, namely, particle‐level scale (∼1 μm) and macroscopic scale (∼several cm). In situ Raman studies revealed a discrete mode of phase transformation at the particle level. Besides, the preferred electrochemical transport network, particularly the carbon content, was found to govern the sequence of phase transformation among particles. In contrast, at the macroscopic level, studies conducted at four different discharge rates showed a continuous but delayed phase transformation. These findings uncovered the intricate phase transformation in LiFePO4 and potentially offer valuable insights into optimizing the length‐scale‐dependent properties of battery materials.
A New Process for Maleic Anhydride Synthesis from a Renewable Building Block: The Gas‐Phase Oxidehydration of Bio‐1‐butanol
Authors: Pavarelli Giulia , Velasquez Ochoa Juliana , Caldarelli Aurora , Puzzo Francesco , Cavani Fabrizio , Dubois Jean‐Luc 
Published: June 2015

AbstractWe investigated the synthesis of maleic anhydride by oxidehydration of a bio‐alcohol, 1‐butanol, as a possible alternative to the classical process of n‐butane oxidation. A vanadyl pyrophosphate catalyst was used to explore the one‐pot reaction, which involved two sequential steps: 1) 1‐butanol dehydration to 1‐butene, catalysed by acid sites, and 2) the oxidation of butenes to maleic anhydride, catalysed by redox sites. A non‐negligible amount of phthalic anhydride was also formed. The effect of different experimental parameters was investigated with chemically sourced 1‐butanol, and the results were then confirmed by using genuinely bio‐sourced 1‐butanol. In the case of bio‐1‐butanol, however, the purity of the product remarkably affected the yield of maleic anhydride. It was found that the reaction mechanism includes the oxidation of butenes to crotonaldehyde and the oxidation of the latter to either furan or maleic acid, both of which are transformed to produce maleic anhydride.
Rapid phenotypic fingerprinting of cell products by robust measurement of ubiquitous surface markers
Authors: Baradez Marc‐Olivier , Lekishvili Tamara , Marshall Damian 
Published: June 2015

AbstractFlow cytometry is one of the most versatile and powerful approach for the quantitative analysis of cell suspensions. With widespread applications in basic and clinical research, its commonest use is in the detection of cell populations labelled against markers specific for a particular phenotype. In this study, we aimed to expand the potential of flow cytometry by describing a method based on robust automated quantification of ubiquitous cell surface markers. We demonstrate that automatic fluorescence standardization combined with whole cell population analysis yields highly reproducible results and can alleviate many of the difficulties associated with conventional analyses. This new generic approach is very flexible for quantifying the uniqueness of entire cell populations regardless of their composition. It provides quantitative phenotypic fingerprints rapidly, does not incorporate subjective factors, is more amenable to standardization, and is easily transferable across a wide diversity of applications, such as quality control for cell manufacture and authentication of cell products. © 2015 International Society for Advancement of Cytometry
An Optimized Bioassay for Mucin1 Detection in Serum Samples
Authors: Florea Anca , Ravalli Andrea , Cristea Cecilia , Săndulescu Robert , Marrazza Giovanna 
Published: June 2015

AbstractTwo simple and sensitive electrochemical approaches for Mucin1 (MUC1) tumor marker using magnetic beads coupling screen‐printed arrays were developed. The single‐use bioassays are based on a sandwich format in which aptamers or antibodies were coupled respectively to Streptavidin or Protein G‐modified magnetic beads. The bioreceptor‐modified beads are used to capture the MUC1 protein from the sample and sandwich assay is performed by the addition of a labeled secondary aptamer or antibody. The enzyme alkaline phosphatase and its substrate (1‐naphthyl phosphate) are then used for the electrochemical detection by differential pulse voltammetry (DPV). The analytical performances of the designed bioassays were compared in terms of sensitivity, selectivity and reproducibility. Using the optimized conditions, a linear range from 0 to 0.28 nM was obtained, with 0.19 nM LOD using antibody‐based and 0.07 nM LOD using aptamer‐based sandwich assay in MUC1 buffered solutions. The results also showed that the aptamer‐based approach exhibited higher selectivity for MUC1, allowing the detection of the protein in complex matrices. The developed aptasensor for MUC1 detection was applied on serum samples obtained from cancer patients, providing promising perspectives for clinical applications.
Fabrication of Silver Nanoparticles Decorated on Activated Screen Printed Carbon Electrode and Its Application for Ultrasensitive Detection of Dopamine
Authors: Palanisamy Selvakumar , Thirumalraj Balamurugan , Chen Shen‐Ming , Ajmal Ali M. , Muthupandi K. , Emmanuel R. , Prakash P. , Al‐Hemaid Fahad M. A. 
Published: June 2015

AbstractIn the present study, we report the fabrication of silver nanoparticles (AgNPs) decorated on activated screen printed carbon electrode (ASPCE). The AgNPs were prepared by using Justicia glauca leaf extract as a reducing and stabilizing agent and the ASPCE was prepared by a simple electrochemical activation of screen printed carbon electrode (SPCE). The ASPCE/AgNPs shows a reversible electrochemical behaviour with enhanced response for DA than that of other modified SPCEs. Under optimum conditions, the electrochemical oxidation current response of DA is linear over the concentration range from 0.05 to 45.35 µM. The limit of detection is found as 0.017 µM with a high sensitivity of 7.85 µA µM−1 cm−2.
Electrocatalytic Oxidation of Acyclovir on Poly(p‐Aminobenzene Sulfonic Acid) Film Modified Glassy Carbon Electrode
Authors: Can Saide , Yilmaz Selehattin , Saglikoglu Gulsen , Sadikoglu Murat , Menek Necati 
Published: June 2015

AbstractAcyclovir is an antiviral effective drug active compound. A glassy carbon electrode (GCE) was modified with an electropolymerized film of p‐aminobenzene sulfonic acid (p‐ABSA) in phosphate buffer solution (PBS). The polymer film‐modified electrode was used to electrochemically detect acyclovir. Polymer film showed excellent electrocatalytic activity for the oxidation of acyclovir. The anodic peak potential value of the acyclovir at the poly(p‐ABSA) modified glassy carbon electrode was 950 mV obtained by DPV. A linear calibration curve for DPV analysis was constructed in the acyclovir concentration range 2×10−7–9×10−6 mol L−1. Limit of detection (LOD) and limit of quantification (LOQ) were obtained as 5.57×10−8 and 1.85×10−7 mol L−1 respectively. The proposed method exhibits good recovery and reproducibility.
Nitric oxide regulates cell behavior on an interactive cell‐derived extracellular matrix scaffold
Authors: Xing Qi , Zhang Lijun , Redman Travis , Qi Shaohai , Zhao Feng 
Published: June 2015

AbstractDuring tissue injury and wound healing process, there are dynamic reciprocal interactions among cells, extracellular matrix (ECM), and mediating molecules which are crucial for functional tissue repair. Nitric oxide (NO) is one of the key mediating molecules that can positively regulate various biological activities involved in wound healing. Various ECM components serve as binding sites for cells and mediating molecules, and the interactions further stimulate cellular activities. Human mesenchymal stem cells (hMSCs) can migrate to the wound site and contribute to tissue regeneration through differentiation and paracrine signaling. The objective of this work was to investigate the regulatory effect of NO on hMSCs in an interactive ECM‐rich microenvironment. In order to mimic the in vivo stromal environment in wound site, a cell‐derived ECM scaffold that was able to release NO within the range of in vivo wound fluid NO level was fabricated. Results showed that the micro‐molar level of NO released from the ECM scaffold had an inhibitory effect on cellular activities of hMSCs. The NO impaired cell growth, altered cell morphology, disrupted the F‐actin organization, also decreased the expression of focal adhesion related molecules integrin α5 and paxillin. These results may contribute to the elucidation of how NO acts on hMSCs in wound healing process. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3807–3814, 2015.
Simple and Sensitive Voltammetric Determination of Esculetin Using Electrochemically Reduced Graphene Oxide Modified Electrode
Authors: Li Yinfeng , Li Ying , Li Kunjing , Ye Baoxian 
Published: June 2015

AbstractA simple and sensitive voltammetric sensor for esculetin, based on electrochemically reduced graphene oxide film modified glassy carbon electrode, was reported for the quantitative determination of esculetin in the Chinese traditional herbal drug Viola yedoensis Makino. Electrochemical impedance spectroscopy and scanning electron microscopy were employed to study the characteristic of the graphene oxide film. The electrochemical behavior of esculetin on this sensor was investigated in pH 3.0 phosphate buffer solution by cyclic voltammetry. Significant advantages were achieved by the excellent conductivity and the high surface‐to‐volume ratio of electrochemically reduced graphene oxide. A calibration plot of oxidation peak currents versus esculetin concentrations was linear in the range of 4.0 ξ 10‐8 mol L‐1 to 5.0 ξ 10‐6 mol L‐1 with a detection limit of 2.0 ξ 10‐8 mol L‐1. The practical application of the present sensor was demonstrated by determining the concentration of esculetin in real sample with no interference.
Quinine Derivative Bonded the Hybrid Mesoporous Silicas Chiral Stationary Phase: Preparation and Application in Liquid Chromatography
Authors: Zhou Jie , Du Qiuzheng , Sun Fang , Zheng Xuxia , Chen Shanshan , Luo Pei 
Published: June 2015

AbstractA novel chiral stationary phase (QN‐APEPMOs) was synthesized by immobilizing quinine derivative (QN) onto spherical ammoniapropyl‐functionalized ethane‐bridged periodic mesoporous organosilicas (APEPMOs). The mesoporous material was prepared by a one‐step co‐condensation of 1,2‐bis (triethoxysilyl) ethane (BTSE) and 3‐ammoniapropyl triethoxysilane (KH‐550) using cetyltrimethyl‐ammoniumchlorine (C18TACl) as a template with the aid of ethanol (co‐solvent) in basic medium. And O‐9‐(tert‐butyl carbamoyl) quinine derivative was immobilized on APEPMOs particles through the vinylic double bond. All of the products were characterized. The results showed that APEPMOs were perfect substrates for chiral stationary phase (CSP) and quinine moieties have been successfully immobilized onto the silica gel. To evaluate the chiral discrimination ability of the synthesized CSP, some analytes have been investigated on QN‐APEPMOs in high performance liquid chromatography (RP‐HPLC) in reversed phase. Three acidic compounds were baseline separated. The results demonstrated that QN‐APEPMOs had high selectivity for acidic compounds such as: carboxylic and sulfonic acids. And the reproducibility of the chiral column was good, while the stability was not very good.
Pharmacokinetics and pharmacodynamics of a human monoclonal anti‐FGF23 antibody (KRN23) in the first multiple ascending‐dose trial treating adults with X‐linked hypophosphatemia
Authors: Zhang Xiaoping , Imel Erik A. , Ruppe Mary D. , Weber Thomas J. , Klausner Mark A. , Ito Takahiro , Vergeire Maria , Humphrey Jeffrey , Glorieux Francis H. , Portale Anthony A. , Insogna Karl , Carpenter Thomas O. , Peacock Munro 
Published: June 2015

AbstractIn X‐linked hypophosphatemia (XLH), serum fibroblast growth factor 23 (FGF23) is increased and results in reduced renal maximum threshold for phosphate reabsorption (TmP), reduced serum inorganic phosphorus (Pi), and inappropriately low normal serum 1,25 dihydroxyvitamin D (1,25[OH]2D) concentration, with subsequent development of rickets or osteomalacia. KRN23 is a recombinant human IgG1 monoclonal antibody that binds to FGF23 and blocks its activity. Up to 4 doses of KRN23 were administered subcutaneously every 28 days to 28 adults with XLH. Mean ± standard deviation KRN23 doses administered were 0.05, 0.10 ± 0.01, 0.28 ± 0.06, and 0.48 ± 0.16 mg/kg. The mean time to reach maximum serum KRN23 levels was 7.0 to 8.5 days. The mean KRN23 half‐life was 16.4 days. The mean area under the concentration–time curve (AUCn) for each dosing interval increased proportionally with increases in KRN23 dose. The mean intersubject variability in AUCn ranged from 30% to 37%. The area under the effect concentration–time curve (AUECn) for change from baseline in TmP per glomerular filtration rate, serum Pi, 1,25(OH)2D, and bone markers for each dosing interval increased linearly with increases in KRN23 AUCn. Linear correlation between serum KRN23 concentrations and increase in serum Pi support KRN23 dose adjustments based on predose serum Pi concentration. © 2015 The Authors. The Journal of Clinical Pharmacology Published by Wiley Periodicals, Inc. on behalf of American College of Clinical Pharmacology