bluuebunny/crossref_metadata_2025_split huggingface dataset:
Typhoon-Position-Oriented Sensitivity Analysis. Part I: Theory and Verification
Authors: Ito Kosuke , Wu Chun-Chieh 
Published: February 2013

Abstract
A new sensitivity analysis method is proposed for the ensemble prediction system in which a tropical cyclone (TC) position is taken as a metric. Sensitivity is defined as a slope of linear regression (or its approximation) between state variable and a scalar representing the TC position based on ensemble simulation. The experiment results illustrate important regions for ensemble TC track forecast. The typhoon-position-oriented sensitivity analysis (TyPOS) is applied to Typhoon Shanshan (2006) for the verification time of up to 48 h. The sensitivity field of the TC central latitude with respect to the vorticity field obtained from large-scale random initial perturbation is characterized by a horizontally tilted pattern centered at the initial TC position. These sensitivity signals are generally maximized in the middle troposphere and are far more significant than those with respect to the divergence field. The results are consistent with the sensitivity signals obtained from existing methods. The verification experiments indicate that the signals from TyPOS quantitatively reflect an ensemble-mean position change as a response to the initial perturbation. Another experiment with Typhoon Dolphin (2008) demonstrates the long-term analysis of forecast sensitivity up to 96 h. Several additional tests have also been carried out to investigate the dependency among ensemble members, the impacts of using different horizontal grid spacing, and the effectiveness of ensemble-Kalman-filter-based perturbations.
Multiyear Predictions of North Atlantic Hurricane Frequency: Promise and Limitations
Authors: Vecchi Gabriel A. , Msadek Rym , Anderson Whit , Chang You-Soon , Delworth Thomas , Dixon Keith , Gudgel Rich , Rosati Anthony , Stern Bill , Villarini Gabriele , Wittenberg Andrew , Yang Xiasong , Zeng Fanrong , Zhang Rong , Zhang Shaoqing 
Published: February 2013

AbstractRetrospective predictions of multiyear North Atlantic Ocean hurricane frequency are explored by applying a hybrid statistical–dynamical forecast system to initialized and noninitialized multiyear forecasts of tropical Atlantic and tropical-mean sea surface temperatures (SSTs) from two global climate model forecast systems. By accounting for impacts of initialization and radiative forcing, retrospective predictions of 5- and 9-yr mean tropical Atlantic hurricane frequency show significant correlations relative to a null hypothesis of zero correlation. The retrospective correlations are increased in a two-model average forecast and by using a lagged-ensemble approach, with the two-model ensemble decadal forecasts of hurricane frequency over 1961–2011 yielding correlation coefficients that approach 0.9. These encouraging retrospective multiyear hurricane predictions, however, should be interpreted with care: although initialized forecasts have higher nominal skill than uninitialized ones, the relatively short record and large autocorrelation of the time series limits confidence in distinguishing between the skill caused by external forcing and that added by initialization. The nominal increase in correlation in the initialized forecasts relative to the uninitialized experiments is caused by improved representation of the multiyear tropical Atlantic SST anomalies. The skill in the initialized forecasts comes in large part from the persistence of a mid-1990s shift by the initialized forecasts, rather than from predicting its evolution. Predicting shifts like that observed in 1994/95 remains a critical issue for the success of multiyear forecasts of Atlantic hurricane frequency. The retrospective forecasts highlight the possibility that changes in observing system impact forecast performance.
The Transient versus the Equilibrium Response of Sea Ice to Global Warming
Authors: Li Chao , Notz Dirk , Tietsche Steffen , Marotzke Jochem 
Published: February 2013

Abstract
To examine the long-term stability of Arctic and Antarctic sea ice, idealized simulations are carried out with the climate model ECHAM5/Max Planck Institute Ocean Model (MPI-OM). Atmospheric CO2 concentration is increased over 2000 years from preindustrial levels to quadrupling, is then kept constant for 5940 years, is afterward decreased over 2000 years to preindustrial levels, and is finally kept constant for 3940 years.
Despite these very slow changes, the sea ice response significantly lags behind the CO2 concentration change. This lag, which is caused by the ocean's thermal inertia, implies that the sea ice equilibrium response to increasing CO2 concentration is substantially underestimated by transient simulations. The sea ice response to CO2 concentration change is not truly hysteretic and is in principle reversible.
The authors find no lag in the evolution of Arctic sea ice relative to changes in annual-mean Northern Hemisphere surface temperature. The summer sea ice cover changes linearly with respect to both CO2 concentration and temperature, while the Arctic winter sea ice cover shows a rapid transition to a very low sea ice coverage. This rapid transition of winter sea ice is associated with a sharply enhanced ice–albedo feedback and a sudden onset of convective-cloud feedback in the Arctic.
The Antarctic sea ice cover retreats continuously without any rapid transition during the warming. Compared to Arctic sea ice, Antarctic sea ice shows a much more strongly lagged response to changes in CO2 concentration. It even lags behind the surface temperature change, which is caused by a different response of ocean deep convection during the warming and the cooling periods.
Reconstruction of Osteochondral Lesions of the Talus With Autologous Spongiosa Grafts and Autologous Matrix-Induced Chondrogenesis
Authors: Valderrabano Victor , Miska Matthias , Leumann André , Wiewiorski Martin 
Published: February 2013

Background: Osteochondral lesions (OCLs) of the talus are a common entity in sports orthopaedics. There are several operative techniques with a good outcome on follow-up examinations. However, limitations such as sacrificing healthy cartilage (osteochondral autograft transfer system [OATS], mosaicplasty), multiple-stage operative procedures (matrix-induced autologous chondrocyte transplantation [MACI], autologous chondrocyte implantation [ACI]), high costs (ACI, allograft), and limited availability (allograft) do remain and reflect potential drawbacks of the currently used techniques. Purpose: To describe a novel operative technique for the treatment of OCLs of the talus in the form of an economically efficient, 1-step procedure combining OCL debridement, spongiosaplasty, and sealing of the OCL area with a collagen matrix. Study Design: Case series; Level of evidence, 4. Methods: Twenty-six patients underwent surgery receiving a modified autologous matrix-induced chondrogenesis (AMIC)–aided repair of OCLs of the talus consisting of debridement, autologous grafting, and sealing of the defect with a collagen scaffold. Ligament repair was performed in 17 of 26 cases. A corrective calcaneal osteotomy was performed in 16 of 26 cases. Clinical and radiological assessment was performed before and a minimum of 24 months after surgery (mean, 31 months; range, 24-54 months). Clinical examination included the American Orthopaedic Foot and Ankle Society (AOFAS) ankle score and the visual analog scale (VAS) for pain. Radiological imaging included single-photon emission computed tomography–computed tomography (SPECT-CT) and magnetic resonance imaging (MRI). The magnetic resonance observation of cartilage repair tissue (MOCART) score was applied, and sport activity was documented. Results: The AOFAS ankle score improved significantly from a mean of 60 points preoperatively (range, 17-79 points) to 89 points (range, 61-100 points) postoperatively ( P < .01). The preoperative pain score averaged 5 (range, 2-8), improving to an average of 1.6 (range, 0-7) postoperatively ( P < .01). The MOCART score for cartilage repair tissue on postoperative MRI averaged 62 points (range, 20-95 points). Complete filling of the defect at the level of the surrounding cartilage was found in 35%, and complete filling with a hypertrophic cartilage layer was found in 50% of the patients. Normal signal intensity of the repair tissue compared with the adjacent native cartilage was seen in 15%, with nearly normal activity in 69%. Nineteen patients (73%) participated in sports before the onset of symptoms compared with 3 (12%) at the time of surgery. The number increased to 16 patients (62%) at postoperative follow-up. Conclusion: The modified AMIC procedure is safe for the treatment of OCLs in the ankle with overall good clinical and MRI results.
“KYDS”: an innovative service addressing the health needs of youth and families in an Australian community
Authors: Citer David , Carlson Kym , McClellan Danni , McGaw Pam , Walter Sophie , Bennett David , Walter Garry 
Published: February 2013

Objective: To describe the evolution of KYDS Youth Development Service (“KYDS”), a pioneering youth service in Sydney, Australia, its range of programs and the challenges that it has faced. Conclusions: KYDS emerged in the context of the escalating presentation of mental health and other problems affecting young people, and the desire of a local community to provide a service for its youth. The service has expanded since opening in 2005 and now includes, among other elements, therapy for referred clients, parent educational forums, and group programs such as a young women’s program and a program for teenagers with parents affected by mental illness. The challenges for the service include financial pressures, service expansion, and how to best complement other services in the public and private sector.
Correction of Multiplanar Deformity of the Second Toe With Metatarsophalangeal Release and Extensor Brevis Reconstruction
Authors: Ellis Scott J. , Young Elizabeth , Endo Yoshimi , Do Huong , Deland Jonathan T. 
Published: February 2013

Background: Surgical treatment of multiplanar (varus and dorsal angulation) second toe deformities due to degenerative instability can lead to recurrence, stiffness, and pain. The goal of this study was to evaluate the short-term outcomes associated with a new technique using an extensor digitorum brevis (EDB) tendon reconstruction to correct such deviation of the second metatarsophalangeal (MTP) joint. Methods: Ten patients (10 female, 0 male) with 11 operated second toes having undergone correction with a reconstruction using the EDB tendon were assessed at an average of 18.5 months (range, 12-34) after surgery. The technique was indicated when MTP and medial partial plantar plate release alone were not sufficient to correct multiplanar deformity. Radiographic parameters (AP and lateral metatarsal-proximal phalanx angles), physical exam (MTP joint range of motion), and subjective outcomes (the Foot and Ankle Outcome Score [FAOS]) were assessed. A toe-specific survey captured more detailed subjective information, including patient satisfaction. Results: Preoperatively, the average MTP joint angle was 4.5 degrees in the varus direction, which changed to 14.2 degrees in the valgus direction postoperatively. On exam, the average MTP joint range of motion was 60.9 ± 11.6 degrees dorsiflexion and 11.1 ± 2.5 degrees plantarflexion. Postoperative FAOS scores demonstrated an average of 89.9 ± 9.8 for the symptoms domain. In all, 9 of 11 patients were either highly satisfied or moderately satisfied (none dissatisfied). Conclusion: With the stated limitations, the EDB tendon reconstruction technique described in conjunction with collateral ligament and partial plantar plate release provided powerful correction. Minimal release of the medial plantar plate and not overtightening the reconstruction are recommended to avoid shifting the toe in the opposite direction. Level of Evidence: Level IV, retrospective study.
Ilizarov External Frame Technique for Pirogoff Amputations With Ankle Disarticulation and Tibiocalcaneal Fusion
Authors: Gessmann Jan , Citak Mustafa , Fehmer Tobias , Schildhauer Thomas Armin , Seybold Dominik 
Published: February 2013

Background: The high rates of wound failure, persisting infection, and nonunion of the tibiocalcaneal arthrodesis are the main reasons why the Pirogoff ankle disarticulation is rarely used for limb salvage. Use of the Ilizarov external frame has increased our fusion rate. The purpose of this study was to review our experience with the use of the Ilizarov external frame as a technique for Pirogoff amputations with ankle disarticulation and tibiocalcaneal fusion. Methods: Twenty-four patients (median age, 57.4 years; range, 29-76 years) underwent a Pirogoff amputation with Ilizarov external frame use between January 2004 and June 2011. The most common indications were gangrene with uncontrollable infection due to Charcot arthropathy or chronic osteomyelitis. Four patients had sustained crush injuries of the foot. All patients were clinically and radiographically followed for a minimum of 12 months. Additionally, 15 patients were evaluated using the Taniguchi rating scale for Pirogoff amputations after a mean follow-up of 44.9 months (range, 12-86 months). Results: In 21 patients (87.5%), a well-healed Pirogoff stump was achieved after a mean external fixation time of 18.1 weeks (range, 12.7-26.6 weeks). Impaired vascular perfusion was found to be the limiting factor for successful wound healing and an overall successful Pirogoff amputation. According to the Taniguchi scale, 67% of the patients achieved good or excellent functional results. Fair (27%) and poor (6%) results were observed only in the diabetic patients. Conclusion: Using the Ilizarov external frame allowed safe fixation and a high success rate, even in neuropathic feet. The frame allowed for immediate weight-bearing and soft tissue control; however, frame-associated complications were common and could result in revision surgery. Level of Evidence: Level IV, case series.
Open Ankle Arthrodeses via an Anterior Approach
Authors: Gordon David , Zicker Robyn , Cullen Nicholas , Singh Dishan 
Published: February 2013

Background: In open ankle arthrodesis, debate remains as to which surgical approach and fixation devices should be used. The purpose of this study was to identify union, complication, and patient satisfaction rates of ankle fusions performed at our institution, using the plane between extensor hallucis longus and tibialis anterior with medial tibiotalar screw internal fixation. Materials and Methods: A retrospective review was performed of all isolated primary fusions between 2005 and 2009. Eighty-two ankles were identified in 73 patients. All patient records were reviewed, and 57 patients (65 ankles) attended for clinical evaluation and scoring. Age range at surgery was 18 to 75 years (mean, 56.1 years); 8 patients were smokers. Diagnoses were trauma in 52 patients (63%), osteoarthritis in 17, rheumatoid arthritis in 7, Charcot-Marie-Tooth disease in 3, congenital talipes equinovarus in 2, and talar avascular necrosis in 1. Follow-up range was 7 months to 8.3 years (mean, 4 years). Results: Time to union ranged from 8 to 39 weeks (mean, 13.3) with a union rate of 100%. The AOFAS range was 12 to 93 (mean, 70). Eighty percent were “very satisfied” or “satisfied.” Major complication rate was 14.6%: 7 malalignments; 3 wound problems; 2 complex regional pain syndrome; and 2 delayed unions, both smokers. Conclusions: An excellent union rate, high patient satisfaction, and low complication rate were achieved with this technique. Varus malalignment and persistent pain resulted in dissatisfaction. Many patients remained highly active, and bilaterally fused patients functioned as well as unilateral ones. Level of Evidence: Level IV, retrospective case series.
Deletion of pic results in decreased virulence for a clinical isolate of Shigella flexneri2a from China
Authors: Zhang Junqi , Qian Lisheng , Wu Yang , Cai Xia , Li Xueping , Cheng Xunjia , Qu Di 
Published: February 2013

Abstract

Background

Shigella is a major pathogen responsible for bacillary dysentery, a severe form of shigellosis. Severity of the disease depends on the virulence of the infecting strain. Shigella pathogenicity is a multi-gene phenomenon, involving the participation of genes on an unstable large virulence plasmid and chromosomal pathogenicity islands.

Results
A multiplex PCR (mPCR) assay was developed to detect S. flexneri 2a from rural regions of Zhengding (Hebei Province, China). We isolated and tested 86 strains using our mPCR assay, which targeted the ipaH, ial and set1B genes. A clinical strain of S. flexneri 2a 51 (SF51) containing ipaH and ial, but lacking set1B was found. The virulence of this strain was found to be markedly decreased. Further testing showed that the SF51 strain lacked pic. To investigate the role of pic in S. flexneri 2a infections, a pic knockout mutant (SF301-∆ pic) and two complementation strains, SF301-∆ pic/pPic and SF51/pPic, were created. Differences in virulence for SF51, SF301-∆ pic, SF301-∆ pic/pPic, SF51/pPic and S. flexneri 2a 301 (SF301) were compared. Compared with SF301, both SF51 and SF301-∆ pic exhibited lower levels of Hela cell invasion and resulted in reduced keratoconjunctivitis, with low levels of tissue damage seen in murine eye sections. The virulence of SF301-∆ pic and SF51 was partially recovered in vitro and in vivo through the addition of a complementary pic gene.

Conclusions
The pic gene appears to be involved in an increase in pathogenicity of S. flexneri 2a. This gene assists with bacterial invasion into host cells and alters inflammatory reactions.
Vitamin B6 deficiency and diseases in elderly people – a study in nursing homes
Authors: Kjeldby Ida K , Fosnes Gunvor S , Ligaarden Solveig C , Farup Per G 
Published: February 2013

Abstract

Background
Vitamin deficiency is a cause of health related problems in elderly people. The aims were to study associations between vitamin B6 (B6) and diseases (primarily functional gastrointestinal disorders) in elderly people in nursing homes, the prevalence of B6 deficiency and factors associated with B6 deficiency.

Methods
This cross-sectional study included residents in nursing homes. Demographics, nutritional status (Mini Nutritional Assessment, MNA® ), physical activity, activity of daily living (Katz Index), dietary habits, use of drugs, and psychiatric and somatic diseases were recorded. A blood sample was collected for haematological and biochemical screening, including B6 (p-PLP); p-PLP values < 20 nmol/l indicates B6 deficiency. The results are given as mean (SD).

Results
Sixty-one residents (men/women: 22/39) with an age of 85.3 (6.8) years and BMI 25.7 (4.5) kg/m2 were included. Malnutrition and risk of malnutrition were present in 11.5% and 61% respectively. Dietary intake of B6 (mg/day) in men and women were 1.60 (0.30) and 1.18 (0.31) (recommended 1.6 and 1.2 respectively), and 14 (23%) used B6 supplements. Median p-PLP was 20.7 (range <4.0-175.8), 30 subjects (49%) had B6 deficiency. B6 deficiency was associated with old age, low s-alanine aminotransferase and s-albumin, elevated s-homocysteine and inactivity (p-values 0.01-0.03). There were no clinically significant associations between B6 deficiency and somatic or psychiatric disorders, and B6 deficiency was not observed in subjects given B6 supplements.

Conclusions
Half of the residents had vitamin B6 deficiency. Vitamin supplement was effective prophylaxis for deficiency and should be recommended to all elderly people in nursing homes.
A case-control study of physical activity patterns and risk of non-fatal myocardial infarction
Authors: Gong Jian , Campos Hannia , Fiecas Joseph Mark A , McGarvey Stephen T , Goldberg Robert , Richardson Caroline , Baylin Ana 
Published: February 2013

Abstract

Background
The interactive effects of different types of physical activity on cardiovascular disease (CVD) risk have not been fully considered in previous studies. We aimed to identify physical activity patterns that take into account combinations of physical activities and examine the association between derived physical activity patterns and risk of acute myocardial infarction (AMI).

Methods
We examined the relationship between physical activity patterns, identified by principal component analysis (PCA), and AMI risk in a case-control study of myocardial infarction in Costa Rica (N=4172), 1994-2004. The component scores derived from PCA and total METS were used in natural cubic spline models to assess the association between physical activity and AMI risk.

Results
Four physical activity patterns were retained from PCA that were characterized as the rest/sleep, agricultural job, light indoor activity, and manual labor job patterns. The light indoor activity and rest/sleep patterns showed an inverse linear relation (P for linearity=0.001) and a U-shaped association (P for non-linearity=0.03) with AMI risk, respectively. There was an inverse association between total activity-related energy expenditure and AMI risk but it reached a plateau at high levels of physical activity (P for non-linearity=0.01).

Conclusions
These data suggest that a light indoor activity pattern is associated with reduced AMI risk. PCA provides a new approach to investigate the relationship between physical activity and CVD risk.
Targeted inhibition of heat shock protein 90 disrupts multiple oncogenic signaling pathways, thus inducing cell cycle arrest and programmed cell death in human urinary bladder cancer cell lines
Authors: Karkoulis Panagiotis K , Stravopodis Dimitrios J , Konstantakou Eumorphia G , Voutsinas Gerassimos E 
Published: February 2013

AbstractBackgroundGeldanamycin (GA) can be considered a relatively new component with a promising mode of action against human malignancies. It specifically targets heat shock protein 90 (Hsp90) and interferes with its function as a molecular chaperone.MethodsIn this study, we have investigated the effects of geldanamycin on the regulation of Hsp90-dependent oncogenic signaling pathways directly implicated in cell cycle progression, survival and motility of human urinary bladder cancer cells. In order to assess the biological outcome of Hsp90 inhibition on RT4 (grade I) and T24 (grade III) human urinary bladder cancer cell lines, we applied MTT assay, FACS analysis, Western blotting, semi-quantitative (sq) RT-PCR, electrophoretic mobility shift assay (EMSA), immunofluorescence and scratch-wound assay.ResultsWe have herein demonstrated that, upon geldanamycin treatment, bladder cancer cells are prominently arrested in the G1 phase of cell cycle and eventually undergo programmed cell death via combined activation of apoptosis and autophagy. Furthermore, geldanamycin administration proved to induce prominent downregulation of several Hsp90 protein clients and downstream effectors, such as membrane receptors (IGF-IR and c-Met), protein kinases (Akt, IKKα, IKKβ and Erk1/2) and transcription factors (FOXOs and NF-κΒ), therefore resulting in the impairment of proliferative -oncogenic- signaling and reduction of cell motility.ConclusionsIn toto, we have evinced the dose-dependent and cell line-specific actions of geldanamycin on cell cycle progression, survival and motility of human bladder cancer cells, due to downregulation of critical Hsp90 clients and subsequent disruption of signaling -oncogenic- integrity.