bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing human‐like collagen accumulation by deleting the major glucose transporter ptsG in recombinant Escherichia coli BL21
Authors: Luo Yan'e , Zhang Tao , Fan Daidi , Mu Tingzhen , Xue Wenjiao , Hui Junfeng , Ma Xiaoxuan 
Published: October 2013

AbstractCollagen has been proven to be a valuable biomedical material for many medical applications. Human‐like collagen (HLC) is a novel important biomedical material with diverse medical applications. In this work, recombinant Escherichia coli BL21 3.7 ∆ptsG was constructed, the characters of ptsG mutant strain were analyzed, and real‐time quantitative polymerase chain reaction (PCR) was applied to investigate the effect of ptsG gene deletion on the transcriptional level of the phosphotransferase system (PTS) genes responsible for glucose transport. The HLC production and cell growth ability were 1.33‐ and 1.24‐fold higher than those of its parent strain in the fermentation medium, respectively, and 1.16‐ and 1.17‐fold in the modified minimal medium individually. The acetate accumulation decreased by 42%–56% compared to its parent strain in the fermentation medium, and 70%–87% in the modified minimal medium. The results of RT‐qPCR showed that the transcriptional level of crr, ptsH, ptsI, and blgF in ptsG mutant all decreased dramatically, which inferred a decrease in the glucose uptake rate, but the transcriptional level of FruB and manX increased slightly, which demonstrated the activation of fructose‐ and mannose‐specific transport pathways in the ptsG mutant. This study demonstrates that ptsG deletion is an effective strategy to reduce acetate accumulation and increase biomass and HLC production.
Acetazolamide triggers death inducing autophagy in T‐47D breast cancer cells
Authors: Mohammadpour Raziye , Safarian Shahrokh , Ejeian Fatemeh , Sheikholya‐Lavasani Zahra , Abdolmohammadi Mohammad Hossein , Sheinabi Nader 
Published: October 2013

AbstractThe inhibitory effects of acetazolamide on the growth and proliferation of epithelial breast cancer cells (T‐47D) were investigated. Analysis of morphological changes indicated little apoptosis in T‐47D cells incubated with acetazolamide, according to data from flow cytometry, DNA laddering, and expression of AIF. However, an increase in caspase‐3 activity was detected in cells. This was concomitant with an increase in DFF45/DFF40 ratio leading to inhibition of caspase‐3 activity, DNA fragmentation and progression of apoptosis. Flow cytometry also confirmed that acetazolamide had no significant effect on cell cycle progression. These results are consistent with lack of change in the expression of cell cycle regulatory proteins p21, p27, cdc2 and cyclinD1. Increased expression of ATG5, p53 and DRAM, along with an increase in BCLN1/Bcl‐2 ratio, indicated that acetazolamide inhibited the proliferation of T‐47D cells by inducing autophagy. Increased expression of PTEN, along with decreased expression of Akt1, also showed that acetazolamide treatment resulted in death inducing autophagy. Collectively the results indicate that autophagy is an adequate mechanism mediating the anti‐cancer effects of acetazolamide in T‐47D cells through engagement of p53/DRAM pathway and attenuation of Akt survival signalling.
Breathing Effect in a Cobalt Phosphonate upon Dehydration/Rehydration: A Single‐Crystal‐to‐Single‐Crystal Study
Authors: Zheng Tao , Clemente‐Juan Juan M. , Ma Jing , Dong Lin , Bao Song‐Song , Huang Jian , Coronado Eugenio , Zheng Li‐Min 
Published: October 2013

AbstractTwo cobalt phosphonates, [Co2(2,2′‐bpy)2(H2O)(pbtcH)] (1) and [Co2(H2O)(pbtcH)(phen)2] (2; pbtcH5=5‐phosphonatophenyl‐1,2,4‐tricarboxylic acid, 2,2′‐bpy=2,2′‐bipyridine, phen=1,10‐phenanthroline), with layer structures are reported. Compound 1 contains O‐C‐O and O‐P‐O bridged tetramers of Co4, which are further connected by pbtcH4− units to form a layer. In compound 2, the cobalt tetramers made up of water‐bridged Co2 dimers and O‐P‐O linkages are connected into a layer by pbtcH4− units. Upon dehydration, compounds 1 and 2 experience single‐crystal‐to‐single‐crystal (SC–SC) structural transformations to form [Co2(2,2′‐bpy)2(pbtcH)] (1 a) and [Co2(pbtcH)(phen)2] (2 a), respectively. The process is reversible in each case. Notably, a breathing effect is observed for 1, accompanied by pore opening and closing due to the reorientation of the coordinated 2,2′‐bpy molecules. The transformation was also monitored by in situ IR measurements. Magnetic studies reveal that antiferromagnetic interactions are mediated between the magnetic centers in compounds 1 and 1 a, whereas ferromagnetic interactions are dominant in compound 2.
Nighttime and Seasonal variations of the Sodium Layer Measured by Lidar at Different Latitudes in China
Authors: Shao‐Hua GONG , Guo‐Tao YANG , Ji‐Yao XU , Xiang‐Hui XUE , Jing JIAO , Da‐Wei TIAN , Sai GUAN , Zheng‐Kuan LIU 
Published: October 2013

AbstractThis study is based on long‐term observations of the sodium layer by lidars at Beijing (40.47°N, 115.97°E), Hefei (31.87°N, 117.23°E) and Hainan (19.99°N, 110.34°E), which are part of the Meridian Project of China. The purpose is to reveal characteristics of nighttime and seasonal variations of the background sodium layer over China along 120°E. The observations from three lidar stations on Dec. 17, 2010 show that, the nighttime variations of the sodium layer are not correlated to each other. The fitted long‐term variations with annual plus semiannual changes reveal that the column abundance of the sodium layer over Beijing and Hefei varied annually, while that over Hainan changed semiannually. The seasonal variations of centroid heights and RMS widths of the sodium layer present apparent semiannual variation tendency, excluding the long‐term change of RMS width for Hainan. Statistics illustrate that the seasonal variations of column density of background sodium layer is correlated with the change of temperature near the mesopause. It has the maximum in winter and minimum in summer, respectively. With the growth of the latitude, the column abundance increases and the annual variation tendency becomes more obvious. There is no clear latitude dependence on the centroid height of the sodium layer, and it changes semiannually with seasons at three locations. The RMS width of the sodium layer is the largest at Beijing (middle and upper latitude) and the least at Hefei (middle latitude) in every month of one year, and it is the medium at Hainan (lower latitude).
Simulation of Number and Distribution of Compass Occultation Events
Authors: Xian‐Yi WANG , Yue‐Qiang SUN , Wei‐Hua BAI , Qi‐Fei DU , Dong‐Wei WANG , Di WU , Qing‐Long YU , Ying HAN 
Published: October 2013

AbstractAs a China's self‐developed satellite navigation system, Compass has broad application prospects in the field of radio occultation. Focused on the task of carrying out Compass occultation observations with a receiver set on LEO satellite, this work developed a simulation system of LEO orbit and Compass constellations, based on which the occultation events can be simulated taking into account different LEO orbit parameters. The effect of LEO satellite's orbit parameters on occultation events’ number and distributions are analyzed considering argument of perigee, right ascension of ascending node, orbit height and inclination. Occultation events for different types of Compass satellites, including GEO, IGSO and MEO, are discussed individually. The characteristics of all three kinds of Compass satellite occultation events were summarized. The results can be referenced when using the Compass navigation system for radio occultation.
TWIST1 is a molecular marker for a poor prognosis in oral cancer and represents a potential therapeutic target
Authors: da Silva Sabrina Daniela , Alaoui‐Jamali Moulay A. , Soares Fernando Augusto , Carraro Dirce Maria , Brentani Helena Paula , Hier Michael , Rogatto Silvia Regina , Kowalski Luiz Paulo 
Published: October 2013

BACKGROUNDLocoregional recurrence and distant metastases are ominous events in patients with advanced oral squamous cell carcinoma (OSCC). The objective of this study was to identify functional biomarkers that are predictive of OSCC progression to metastasis.METHODSThe expression profile of a network of epithelial‐mesenchymal transition (EMT) genes was investigated in a large cohort of patients with progressive OSCC using a complimentary DNA microarray platform coupled to quantitative reverse transcriptase‐polymerase chain reaction and immunohistochemical analyses. Therapeutic potential was investigated in vitro and in vivo using an orthotopic mouse model of metastatic OSCC growing in the tongue microenvironment.RESULTSAmong deregulated EMT genes, the Twist‐related protein 1 (TWIST1) transcription factor and several of its regulated genes were significantly overexpressed across advanced stages of OSCC. This result was corroborated by the clinical observation that Twist1 up‐regulation predicted the occurrence of lymph node and lung metastases as well as poor patient survival. In support of Twist1 as a driver of OSCC progression, the up‐regulation of Twist1 was observed in cells isolated from patients with metastatic OSCC. The inhibition of Twist1 in these metastatic cells induced a potent inhibition of cell invasiveness in vitro as well as progression in vivo.CONCLUSIONSThe current results provide evidence for the prognostic value and therapeutic potential of a network of Twist genes in patients with advanced OSCC. Cancer 2014;120:352–362. © 2013 American Cancer Society.
HER‐2/neu gene amplification in relation to expression of HER2 and HER3 proteins in patients with esophageal adenocarcinoma
Authors: Yoon Harry H. , Sukov William R. , Shi Qian , Sattler Christopher A. , Wiktor Anne E. , Diasio Robert B. , Wu Tsung‐Teh , Jenkins Robert B. , Sinicrope Frank A. 
Published: October 2013

BACKGROUNDHuman epidermal growth factor receptor 2 (HER2) is a therapeutic target in patients with esophageal adenocarcinoma (EAC), with gene amplification used as a selection criterion for treatment, although to the authors' knowledge the concordance between amplification and HER2 protein expression remains undefined in EAC. Furthermore, the association between HER2 and its interacting partner, human epidermal growth factor receptor 3 (HER3), is unknown yet appears to be of potential therapeutic relevance.METHODSPatients with untreated EACs (N = 673) were analyzed for HER2 amplification and polysomy 17 by fluorescence in situ hybridization in parallel with immunohistochemistry (IHC) (IHC scores of 0‐1+, 2+, and 3+). Amplification was defined as HER2/CEP17 ≥ 2. HER3 expression by IHC was analyzed in randomly selected cases (n = 224). IHC and fluorescence in situ hybridization results were compared using least squares linear regression.RESULTSOverall, 17% of the EACs (116 of 673 EACs) were HER2‐amplified with an amplification frequency that was highest among IHC3+ cases (89%) and declined among IHC2+ cases (13%) and IHC0 to IHC1+ cases (4%). Among HER2‐amplified cases, the level of amplification increased linearly with HER2 membranous expression (HER2/CEP17 ratio: 7.9 in IHC3+ and 5.5 in IHC2+ vs 2.8 in IHC0 to IHC1+ [P < .0001]), with 14% of amplified tumors demonstrating absent/faint expression (IHC0 to IHC1+). Polysomy 17 was not found to be associated with HER2 expression. Cytoplasmic HER3 expression was detected in 87% of tumors (195 of 224 tumors) and was found to be significantly associated with better differentiation (P < .0001). Stepwise increases in HER3 expression were associated with higher HER2 expression levels (P = .0019).CONCLUSIONSLevels of HER2 protein expression and amplification were found to be linearly associated and highly concordant. Among amplified tumors with absent/faint expression, the level of amplification was low. Frequent expression of HER3 suggests its relevance as a therapeutic target, and its significant association with HER2 supports ongoing efforts to inhibit HER2/HER3 in patients with EAC. Cancer 2014;120:415–424. © 2013 American Cancer Society.
Seismic response of 20‐story base‐isolated and fixed‐base reinforced concrete structural wall buildings at a near‐fault site
Authors: Calugaru Vladimir , Panagiotou Marios 
Published: October 2013

SUMMARYThis paper investigates numerically the seismic response of six seismically base‐isolated (BI) 20‐story reinforced concrete buildings and compares their response to that of a fixed‐base (FB) building with a similar structural system above ground. Located in Berkeley, California, 2 km from the Hayward fault, the buildings are designed with a core wall that provides most of the lateral force resistance above ground. For the BI buildings, the following are investigated: two isolation systems (both implemented below a three‐story basement), isolation periods equal to 4, 5, and 6 s, and two levels of flexural strength of the wall. The first isolation system combines tension‐resistant friction pendulum bearings and nonlinear fluid viscous dampers (NFVDs); the second combines low‐friction tension‐resistant crosslinear bearings, lead‐rubber bearings, and NFVDs. The designs of all buildings satisfy ASCE 7‐10 requirements, except that one component of horizontal excitation, is used in the 2D nonlinear response history analysis. Analysis is performed for a set of ground motions scaled to the design earthquake and to the maximum considered earthquake (MCE). At both the design earthquake and the MCE, the FB building develops large inelastic deformations and shear forces in the wall and large floor accelerations. At the MCE, four of the BI buildings experience nominally elastic response of the wall, with floor accelerations and shear forces being 0.25 to 0.55 times those experienced by the FB building. The response of the FB and four of the BI buildings to four unscaled historical pulse‐like near‐fault ground motions is also studied. Copyright © 2013 John Wiley & Sons, Ltd.
Clinical presentation and differential diagnosis of dementia with Lewy bodies: a review
Authors: Morra L. F. , Donovick P. J. 
Published: October 2013

BackgroundDementia with Lewy bodies is one of the most prevalent dementia diagnoses. However, differential diagnosis between dementia with Lewy bodies, Alzheimer's disease, and Parkinson's disease with dementia can still be very difficult given the overlap in neuropathology, clinical presentation, cognitive, and neuroanatomical changes.MethodA literature review of dementia with Lewy bodies, Alzheimer's disease, and Parkinson's disease with dementia was conducted using PubMed.Results and ImplicationsAccurate diagnosis of dementia with Lewy bodies is crucial in order to more accurately predict the progression of the disease and negative side effects from pharmacological treatment. The differences and similarities between dementia with Lewy bodies, Alzheimer's disease, and Parkinson's disease with dementia are highlighted in order to aid clinicians in differential diagnosis. Copyright © 2013 John Wiley & Sons, Ltd.
Local label learning (LLL) for subcortical structure segmentation: Application to hippocampus segmentation
Authors: Hao Yongfu , Wang Tianyao , Zhang Xinqing , Duan Yunyun , Yu Chunshui , Jiang Tianzi , Fan Yong ,   for the Alzheimer's Disease Neuroimaging Initiative
Published: October 2013

AbstractAutomatic and reliable segmentation of subcortical structures is an important but difficult task in quantitative brain image analysis. Multi‐atlas based segmentation methods have attracted great interest due to their promising performance. Under the multi‐atlas based segmentation framework, using deformation fields generated for registering atlas images onto a target image to be segmented, labels of the atlases are first propagated to the target image space and then fused to get the target image segmentation based on a label fusion strategy. While many label fusion strategies have been developed, most of these methods adopt predefined weighting models that are not necessarily optimal. In this study, we propose a novel local label learning strategy to estimate the target image's segmentation label using statistical machine learning techniques. In particular, we use a L1‐regularized support vector machine (SVM) with a k nearest neighbor (kNN) based training sample selection strategy to learn a classifier for each of the target image voxel from its neighboring voxels in the atlases based on both image intensity and texture features. Our method has produced segmentation results consistently better than state‐of‐the‐art label fusion methods in validation experiments on hippocampal segmentation of over 100 MR images obtained from publicly available and in‐house datasets. Volumetric analysis has also demonstrated the capability of our method in detecting hippocampal volume changes due to Alzheimer's disease. Hum Brain Mapp 35:2674–2697, 2014. © 2013 Wiley Periodicals, Inc.
Temporal variation of suspended sediment transport in the Koga catchment, North Western Ethiopia and environmental implications
Authors: Yeshaneh Eleni , Eder Alexander , Blöschl Günter 
Published: October 2013

AbstractEvent sediment transport and yield were studied for 45 events in the upstream part of the 260 km2 agricultural Koga catchment that drains to an irrigation reservoir. Discharge and turbidity data were collected over a period of more than a year, accompanied by grab sampling. Turbidity was very well correlated with the sediment concentrations from the samples (r = 0.99), which allowed us to estimate the temporal patterns of sediment concentrations within events. The hysteresis patterns between discharge and sediment concentrations were analysed to provide insight into the different sediment sources. Anticlockwise patterns are the dominant hysteresis patterns in the area, suggesting smaller contributions of suspended sediment from the river channels than from the hillslopes and agricultural areas. Complicated types of hysteresis patterns were mostly observed for long events with multiple peaks. For a given discharge, sediment yields in August and September, when the catchment was almost completely covered with vegetation, were much smaller than during the rest of the rainy season. The hysteresis patterns and timing suggest that the sediment availability from the agricultural areas and hillslopes affects sediment yields more strongly than does peak discharge. Two distinct types of sediment rating curves were observed for the season when the agricultural land was covered with vegetation and when it was not, indicating the dominating contribution of land use/cover to sediment yields in the catchment. The rate of suspended sediment transport in the area was estimated as 25.6 t year−1 ha−1. Copyright © 2013 John Wiley & Sons, Ltd.
Relating pore size variation of poly (ɛ‐caprolactone) scaffolds to molecular weight of porogen and evaluation of scaffold properties after degradation
Authors: Columbus Soumya , Krishnan Lissy K. , Kalliyana Krishnan V. 
Published: October 2013

AbstractThe major challenge in designing a scaffold for fabricating tissue engineered blood vessels is optimization of its microstructure for supporting uniform cellular in‐growth with good mechanical integrity and degradation kinetics suitable for long‐term implantation. In this study, we have investigated the feasibility of varying the pore size of poly(ɛ‐caprolactone) (PCL) scaffold by altering the molecular weight of porogen and studied the effect of degradation on morphological characteristics and mechanical properties of scaffolds by correlating to the extent of degradation. Scaffolds with two different pore sizes were prepared by solvent casting and particulate leaching where poly(ethylene glycol) (PEG) porogens having two molecular weights (3400 and 8000) were used and subjected toin vitrodegradation in phosphate buffered saline (PBS) upto six months. Microcomputed tomography studies of scaffolds revealed narrower pore size distribution when PEG‐3400 was used as porogen and had 78% pores in the 12–24 µ range, whereas incorporation of PEG‐8000 resulted in broader distribution with only 65% pores in the same range. Degradation resulted in scaffolds with narrower pore size distribution to have better retention of morphological and mechanical characteristics compared to scaffolds with broader distribution. Gravimetric and molecular weight studies also showed that scaffold degradation in both cases was only in initial stages after 6 months and PCL scaffolds had potential to be recommended for vascular tissue engineering applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 789–796, 2014.
Methodology of fibroblast and mesenchymal stem cell coating of surgical meshes: A pilot analysis
Authors: Gao Yue , Liu Li‐Jia , Blatnik Jeffrey A. , Krpata David M. , Anderson James M. , Criss Corry N. , Posielski Natasza , Novitsky Yuri W. 
Published: October 2013

AbstractCoating of various synthetic, absorbable, and biologic meshes with mesenchymal stem cells (MSCs) and fibroblasts was analyzed qualitatively and quantitatively. Five hernia meshes—light weight monofilament polypropylene (Soft Mesh), polyester (Parietex‐TET), polylactide composite (TIGR), heavy weight monofilament polypropylene (Marlex), and porcine dermal collagen (Strattice)—were coated with three cell lines: human dermal fibroblasts (HFs), rat kidney fibroblasts (NRKs), and rat MSCs. Cell densities were determined at different time points. Samples also underwent histology and transmission electron microscopic (TEM) analyses. It required HFs 3 weeks to cover the entire mesh, while only 2 weeks for NRKs and MSCs to do so. MSCs had no preference for any of the meshes and produced the highest cell densities on Parietex and TIGR. Substrate‐preference accounted for the significantly lower fibroblast densities on TIGR than Parietex. Fibroblasts failed to coat Marlex. Strattice, which had the least surface area, generated comparable cell densities to Parietex. Both histology and TEM confirmed cell coating of mesh surface. Various prosthetics can be coated by certain cell strains. Both mesh composition and cell preference dramatically influence the coating process. This methodology provides foundation for novel avenues of modulation of host response to various modern synthetic and biologic meshes. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 797–805, 2014.