bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the fluorescence resonance energy transfer between CdS quantum dots and Eosin Y
Authors: Yan Zhengyu , Zhang Zhengwei , Yu Yan , Chen Jianqiu 
Published: June 2014

ABSTRACTWater‐soluble CdS quantum dots (QDs) were prepared using mercaptoacetic acid (TGA) as the stabilizer in an aqueous system. A fluorescence resonance energy transfer (FRET) system was constructed between water‐soluble CdS QDs (donor) and Eosin Y (acceptor). Several factors that impacted the fluorescence spectra of the FRET system, such as pH (3.05–10.10), concentration of Eosin Y (2–80 mg/L) and concentration of CdS QDs (2–80 mg/L), were investigated and refined. Donor‐to‐acceptor ratios, the energy transfer efficiency (E) and the distance (r) between CdS QDs and Eosin Y were obtained. The results showed that a FRET system could be established between water‐soluble CdS QDs and Eosin Y at pH 5.0; donor‐to‐acceptor ratios demonstrated a 1: 8 proportion of complexes; the energy transfer efficiency (E) and the distance (r) between the QDs and Eosin Y were 20.07% and 4.36 nm,respectively. Copyright © 2014 John Wiley & Sons, Ltd.
Insulin receptor antibody‐iduronate 2‐sulfatase fusion protein: Pharmacokinetics, anti‐drug antibody, and safety pharmacology in Rhesus monkeys
Authors: Boado Ruben J. , Ka‐Wai Hui Eric , Zhiqiang Lu Jeff , Pardridge William M. 
Published: June 2014

ABSTRACTMucopolysaccharidosis (MPS) Type II is caused by mutations in the gene encoding the lysosomal enzyme, iduronate 2‐sulfatase (IDS). The majority of MPSII cases affect the brain. However, enzyme replacement therapy with recombinant IDS does not treat the brain, because IDS is a large molecule drug that does not cross the blood‐brain barrier (BBB). To enable BBB penetration, IDS has been re‐engineered as an IgG‐IDS fusion protein, where the IgG domain is a monoclonal antibody (MAb) against the human insulin receptor (HIR). The HIRMAb crosses the BBB via receptor‐mediated transport on the endogenous BBB insulin receptor, and the HIRMAb domain of the fusion protein acts as a molecular Trojan horse to ferry the fused IDS into brain from blood. The present study reports on the first safety pharmacology and pharmacokinetics study of the HIRMAb‐IDS fusion protein. Juvenile male Rhesus monkeys were infused intravenously (IV) weekly for 26 weeks with 0, 3, 10, or 30 mg/kg of the HIRMAb‐IDS fusion protein. The plasma clearance of the fusion protein followed a linear pharmacokinetics profile, which was equivalent either with measurements of the plasma concentration of immunoreactive HIRMAb‐IDS fusion protein, or with assays of plasma IDS enzyme activity. Anti‐drug antibody (ADA) titers were monitored monthly, and the ADA response was primarily directed against the variable region of the HIRMAb domain of the fusion protein. No infusion related reactions or clinical signs of immune response were observed during the course of the study. A battery of safety pharmacology, clinical chemistry, and tissue histopathology showed no signs of adverse events, and demonstrate the safety profile of chronic treatment of primates with 3–30 mg/kg weekly IV infusion doses of the HIRMAb‐IDS fusion protein. Biotechnol. Bioeng. 2014;111: 2317–2325. © 2014 Wiley Periodicals, Inc.
Efficient odd straight medium chain free fatty acid production by metabolically engineered Escherichia coli
Authors: Wu Hui , San Ka‐Yiu 
Published: June 2014

ABSTRACTFree fatty acids (FFAs) can be used as precursors for the production of biofuels or chemicals. Different composition of FFAs will be useful for further modification of the biofuel/biochemical quality. Microbial biosynthesis of even chain FFAs can be achieved by introducing an acyl‐acyl carrier protein thioesterase gene into E. coli. In this study, odd straight medium chain FFAs production was investigated by using metabolic engineered E. coli carrying acyl‐ACP thioesterase (TE, Ricinus communis), propionyl‐CoA synthase (Salmonella enterica), and β‐ketoacyl‐acyl carrier protein synthase III (four different sources) with supplement of extracellular propionate. By using these metabolically engineered E. coli, significant quantity of C13 and C15 odd straight‐chain FFAs could be produced from glucose and propionate. The highest concentration of total odd straight chain FFAs attained was 1205 mg/L by the strain HWK201 (pXZ18, pBHE2), and 85% of the odd straight chain FFAs was C15. However, the highest percentage of odd straight chain FFAs was achieved by the strain HWK201 (pXZ18, pBHE3) of 83.2% at 48 h. This strategy was also applied successfully in strains carrying different TE, such as the medium length acyl‐ACP thioesterase gene from Umbellularia californica. C11 and C13 became the major odd straight‐chain FFAs. Biotechnol. Bioeng. 2014;111: 2209–2219. © 2014 Wiley Periodicals, Inc.
Rapid determination of corticosterone in mouse plasma by ultra fast liquid chromatography‐tandem mass spectrometry
Authors: Li Huan , Liu Xiao , Poh Yanhong , Wu Li , Zhou Qi‐Gang , Cai Bao‐Chang 
Published: June 2014

ABSTRACTMajor depressive disorder is a severe, life‐threatening and highly prevalent psychiatric disorder. A high percentage of people suffering from depression are characterized by hyperactivity of the hypothalamic–pituitary–adrenal axis, resulting in plasma glucocorticoid (cortisol in human and corticosterone in rodent) elevations. Glucocorticoid is a critical molecule in the onset of pathology of depression. A simple, highly sensitive and specific method based on ultra‐fast liquid chromatography–tandem mass spectrometry method has been developed for the quantitation of corticosterone in mouse plasma for the first time, which provides technical support for the high‐throughput measurement for clinical determination of corticosterone in biological samples. Samples were spiked with methanol to precipitate the protein, and then chromatographed on an Agilent Zorbax Eclipse Plus C18 (100 × 2.1 mm,1.8 µm) column by linear gradient elution with methanol and 0.1% formic acid as the mobile phase within 5 min. The detection of corticosterone was performed on ultra‐fast liquid chromatography–triple quadrupole tandem mass spectrometry in the positive ion. The ions [M + H]+ m/z 347.2 → m/z 311.1 for corticosterone and [M + H]+ m/z 363.2 → m/z 327.2 for hydrocortisone (internal standard) were used for quantitative determination. The lower quantification limit for corticosterone was 1 ng/mL. The validated method was successfully applied to the quantitation of corticosterone in mouse plasma. Copyright © 2014 John Wiley & Sons, Ltd.
Simultaneous quantification of catecholamines in rat brain by high‐performance liquid chromatography with on‐line gold nanoparticle‐catalyzed luminol chemiluminescence detection
Authors: Mu Chunlei , Zhang Qi , Wu Dong , Zhang Yunjing , Zhang Qunlin 
Published: June 2014

ABSTRACTA new method based on high‐performance liquid chromatography (HPLC) coupled with on‐line gold nanoparticle‐catalyzed luminol chemiluminescence (CL) detection was developed for the simultaneous quantitation of catecholamines in rat brain. In the present CL system, gold nanoparticles were produced by the on‐line reaction of H2O2, NaHCO3−Na2CO3 (buffer solution of luminol) and HAuCl4. Norepinephrine (NE), epinephrine (EP) and dopamine (DA) could strongly enhance the CL signal of the on‐line gold nanoparticle‐catalyzed luminol system. The UV−visible absorption spectra and transmission electron microscopy studies were carried out, and the CL enhancement mechanism was proposed. Catecholamines promoted the on‐line formation of more gold nanoparticles, which better catalyzed the luminol–H2O2 CL reaction. The good separation of NE, EP and DA was achieved with isocratic elution using a mixture of methanol and 0.2% aqueous phosphoric acid (5:95, v/v) within 8.5 min. Under the optimized conditions, the detection limits, defined as a signal‐to‐noise ratio of 3, were in the range of 1.32–1.90 ng/mL, corresponding to 26.4−38.0 pg for 20 μL sample injection. The recoveries of catecholamines added to rat brain sample were >94.6%, with the precisions <5.5%. The validated HPLC−CL method was successfully applied to determine NE and DA in rat brain without prior sample purification. Copyright © 2014 John Wiley & Sons, Ltd.
A simple, rapid and sensitive liquid chromatography–tandem mass spectrometry method for the determination of dienogest in human plasma and its pharmacokinetic applications under fasting
Authors: Pallapothu Leela Mohan Kumar , Batta Neelima , Pigili Ravi Kumar , Yejella Rajendra Prasad 
Published: June 2014

AbstractA simple, rapid and sensitive analytical method using liquid chromatography coupled to tandem mass spectrometry (LC‐MS/MS) detection with positive ion electrospray ionization was developed for the determination of dienogest in human K2EDTA plasma using levonorgestrel d6 as an internal standard (IS). Dienogest and IS were extracted from human plasma using simple liquid–liquid extraction. Chromatographic separation was achieved on a Zorbax XDB‐Phenyl column (4.6 × 75 mm, 3.5 µm) under isocratic conditions using acetonitrile–5 mm ammonium acetate (70:30, v/v) at a flow rate of 0.60 mL/min. The protonated precursor to product ion transitions monitored for dienogest and IS were at m/z 312.30 → 135.30 and 319.00 → 251.30, respectively. The method was validated with a linearity range of 1.003–200.896 ng/mL having a total analysis time for each chromatograph of 3.0 min. The method has shown tremendous reproducibility with intra‐ and inter‐day precision (coefficient of variation) <3.97 and 6.10%, respectively, and accuracy within ±4.0% of nominal values. The validated method was applied to a pharmacokinetic study in human plasma samples generated after administration of a single oral dose of 2.0 mg dienogest tablets to healthy female volunteers and was proved to be highly reliable for the analysis of clinical samples. Copyright © 2014 John Wiley & Sons, Ltd.
Development of a complete human anti‐human transferrin receptor C antibody as a novel marker of oral dysplasia and oral cancer
Authors: Nagai Kentaro , Nakahata Shingo , Shimosaki Shunsuke , Tamura Tomohiro , Kondo Yuudai , Baba Takashi , Taki Tomohiko , Taniwaki Masafumi , Kurosawa Gene , Sudo Yukio , Okada Seiji , Sakoda Sumio , Morishita Kazuhiro 
Published: June 2014

AbstractOral squamous cell carcinoma (OSCC) is the sixth most common cancer worldwide. Up to 20% of oral dysplasia cases have been suggested to undergo malignant transformation to OSCC; however, there are no methods to predict OSCC development. In this study, to identify the genes associated with oral dysplasia progression, we performed genomic copy number analyses of genomic DNA samples isolated from primary oral dysplasia and OSCC via the microdissection method and found elevated expression of transferrin receptor C (TfR1/TFRC) with genomic amplification in oral dysplasia and OSCC. The expression rate of TFRC in OSCC was significantly higher than that in dysplasia, suggesting that OSCC disease progression might be related to TFRC expression. Additionally, we investigated the in vitro and in vivo impacts of a newly established anti‐human TFRC monoclonal antibody, which was isolated from a human cDNA library using the phage‐display method, on cell proliferation and survival. The anti‐TFRC antibody blocked the interaction between transferrin and TFRC and consequently inhibited iron uptake, leading to the iron deprivation‐mediated suppression of cell growth and induction of apoptosis. Moreover, we demonstrated that the anti‐TFRC antibody efficiently inhibited tumor growth in a murine xenograft OSCC model. Therefore, we suggest our developed complete human anti‐human TFRC antibody as a useful, novel treatment for oral dysplasia and OSCC.
Let‐7 microRNA‐binding‐site polymorphism in the 3′UTR of KRAS and colorectal cancer outcome: a systematic review and meta‐analysis
Authors: Langevin Scott M. , Christensen Brock C. 
Published: June 2014

AbstractThere is a small but growing body of literature regarding the predictive utility of a Let‐7 microRNA‐binding‐site polymorphism in the 3′‐untranslated region (UTR) of KRAS (KRAS‐LCS6) for colorectal cancer outcome, although the results are conflicting. We performed a review and meta‐analysis in an attempt to better clarify this relationship. A PubMed search was conducted to identify all studies reporting on KRAS let‐7 microRNA‐binding site polymorphism (LCS6; rs61764370) and colorectal cancer outcome. Hazard ratios (HR) and corresponding 95% confidence intervals (CI) were extracted or estimated from each manuscript. Log HRs and log CIs were combined across studies using the inverse‐variance weight to calculate fixed‐ and random‐effects summary estimates and corresponding 95% CIs for overall and progression‐free survival. We did not observe any significant association between overall or progression‐free survival, neither when considering all colorectal cancer patients nor for subgroup analyses (metastatic, anti‐EGFR [epidermal growth factor receptor] treatment, or KRAS wild type). There was substantial heterogeneity across studies, overall and among subgroups analyzed. We have found no clear evidence to support an association between the KRAS‐LCS6 genotype and overall or progression‐free survival among colorectal cancer patients, even after conducting subgroup analyses by stage and anti‐EGFR treatment status. This information helps to clarify the confusing body of literature regarding the clinical implications of the KRAS‐LCS6 genetic variant on colorectal cancer outcomes, indicating that it should not be used at the present time to personalize therapeutic strategies (PROSPERO registration number: CRD42013005325).
Synthesis of Different Ruthenium Nickel Bimetallic Nanostructures and an Investigation of the Structure–Activity Relationship for Benzene Hydrogenation to Cyclohexane
Authors: Zhu Lihua , Cao Maohong , Li Li , Sun Hanlei , Tang Yanqing , Zhang Nuowei , Zheng Jinbao , Zhou Hua , Li Yunhua , Yang Lefu , Zhong Chuan‐Jian , Chen Bing H. 
Published: June 2014

AbstractThe catalytic properties of catalysts are generally highly dependent on their nanostructures in most heterogeneous catalytic reactions. Therefore, to acquire targeted catalytic activity, selectivity, and stability, catalysts with a specific nanostructure should be designed and synthesized. Herein, Ru‐Ni bimetallic nanoparticles with different nanostructures, Ru‐Ni alloy, Ru@Ni, and Ru clusters‐on‐Ni on carbon, have been synthesized by annealing Ru‐Ni/C in flowing N2+10 % H2 at different temperatures. The various nanostructures of the Ru‐Ni bimetallic nanoparticles have been characterized and their catalytic behaviors were evaluated using benzene hydrogenation to cyclohexane. The relationship between the Ru‐Ni bimetallic nanostructures and their catalytic performance is presented. It was found that Ru‐Ni alloy/C and Ru clusters‐on‐Ni/C are much more active than Ru@Ni/C. This study also provides a simple method to design and control the nanostructures of the Ru‐Ni bimetallic nanoparticles.
Stereo‐ and Chemoselective Character of Supported CeO2 Catalysts for Continuous‐Flow Three‐Phase Alkyne Hydrogenation
Authors: Vilé Gianvito , Wrabetz Sabine , Floryan Leonard , Schuster Manfred Erwin , Girgsdies Frank , Teschner Detre , Pérez‐Ramírez Javier 
Published: June 2014

AbstractTiO2‐, Al2O3‐, and ZrO2‐supported CeO2 catalysts with different Ce loadings were prepared by wet impregnation of the carriers with an acidified solution of cerium ammonium nitrate. The calcined catalysts were characterized by bulk and surface‐sensitive techniques, which included microcalorimetry, and evaluated in the three‐phase hydrogenation of alkynes under continuous‐flow conditions at variable temperature (293–413 K) and pressure (1–90 bar). A number of acetylenic compounds, which contain terminal or internal triple bonds, conjugated unsaturations, and additional functionalities, were systematically assessed. The results revealed the full stereo‐ and chemoselective character of the ceria catalysts, which outperform the well‐known Lindlar catalyst, and open promising perspectives for the revolutionary use of a cost‐effective oxide for the production of olefinic compounds in the vitamin and fine chemical industries.
Revealing the Fine Details of Functionalized Silica Surfaces by Solid‐State NMR and Adsorption Isotherm Measurements: The Case of Fluorinated Stationary Phases for Liquid Chromatography
Authors: Ciogli Alessia , Simone Patrizia , Villani Claudio , Gasparrini Francesco , Laganà Aldo , Capitani Donatella , Marchetti Nicola , Pasti Luisa , Massi Alessandro , Cavazzini Alberto 
Published: June 2014

AbstractThe structural and chromatographic characterization of two novel fluorinated mesoporous materials prepared by covalent reaction of 3‐(pentafluorophenyl)propyldimethylchlorosilane and perfluorohexylethyltrichlorosilane with 2.5 μm fully porous silica particles is reported. The adsorbents were characterized by solid state 29Si, 13C, and 19F NMR spectroscopy, low‐temperature nitrogen adsorption, elemental analysis (C and F), and various chromatographic measurements, including the determination of adsorption isotherms. The structure and abundance of the different organic surface species, as well as the different silanol types, were determined. In particular, the degree of so‐called horizontal polymerization, that is, Si‐O‐Si bridging parallel to the silica surface due to the reaction, under “quasi‐dry” conditions, of trifunctional silanizing agents with the silica surface was quantified. Significant agreement was found between the information provided by solid‐state NMR, elemental analysis, and excess isotherms regarding the amount of surface residual silanol groups, on the one hand, and the degree of surface functionalization, on the other. Finally, the kinetic performance of the fluorinated materials as separation media for applications in near‐ultrahigh‐performance liquid chromatography was evaluated. At reduced velocities of about 5.5 (ca. 600 bar backpressure at room temperature) with 3 mm diameter columns and toluene as test compound, reduced plate heights on the order of 2 were obtained on columns of both adsorbents.
ChemInform Abstract: Probing the Zintl—Klemm Concept: A Combined Experimental and Theoretical Charge Density Study of the Zintl Phase CaSi.
Authors: Kurylyshyn Iryna M. , Faessler Thomas F. , Fischer Andreas , Hauf Christoph , Eickerling Georg , Presnitz Manuel , Scherer Wolfgang 
Published: June 2014

AbstractThe nature of the chemical bonds in the Zintl phase CaSi is investigated by a combined experimental and theoretical charge density analysis to test the validity of the Zintl‐Klemm concept.
ChemInform Abstract: Synthesis, Structure, and Magnetic Behavior of (LaxCe1‐x)1.33Pt4Ga10 (0 ≤ x ≤ 1).
Authors: Macaluso Robin T. , et al. et al. 
Published: June 2014

AbstractPlate‐like crystals of (LaxCe1‐x)1.33Pt4Ga10 (0 ≤ x ≤ 1) are prepared by flux melting of the elements with excess Ga (alumina crucible in evacuated quartz tube, 1150 °C, 7 h, cooling to 350 °C with a rate of 8 °C/h).
ChemInform Abstract: The Topochemical Pseudomorphosis of a Chloride into a Bismuthide.
Authors: Kaiser Martin , Rasche Bertold , Ruck Michael 
Published: June 2014

AbstractComplete topochemical exchange of Cl‐ ions for Bi in the intermetallic subhalide Bi12Rh3Cl2 is achieved by heterogeneous reaction of crystals with BuLi (70 °C, 2 d) leading to the isostructural metastable intermetallic superconductor Bi14Rh3.
ChemInform Abstract: Structure and Properties of New Gallium‐Containing Polyoxotungstates with Hexanuclear and Tetranuclear Cores.
Authors: von Allmen Kim , Car Pierre‐Emmanuel , Blacque Olivier , Fox Thomas , Mueller Rafael , Patzke Greta R. 
Published: June 2014

AbstractThe new compound (IV) exists with two similar structures, namely with the monoclinic space group C2/c (Z = 2) and with the triclinic space group P$\bar 1$ (Z = 2).
ChemInform Abstract: Catalytic Asymmetric Conjugate Addition of Terminal Alkynes to β‐Trifluoromethyl α,β‐Enones.
Authors: Sanz‐Marco Amparo , Garcia‐Ortiz Andrea , Blay Gonzalo , Pedro Jose R. 
Published: June 2014

AbstractThe first enantioselective conjugate alkynylation of β‐trifluoromethyl α,β‐enones (II) using terminal alkynes (I) and a taniaphos‐Cu(I) complex as catalyst is described.
ChemInform Abstract: An Unprecedented Pd‐Catalyzed trans‐Addition of Boronic Acids to Ynamides.
Authors: Yang Yuanfa , Wang Lina , Zhang Jieni , Jin Yali , Zhu Gangguo 
Published: June 2014

AbstractThe title reaction affords α,β‐disubstituted enamides such as (III), (V), and (VII) in good yields with high regio‐ and stereoselectivity.
ChemInform Abstract: Stereoselective Iron‐Catalyzed Alkyne Hydrogenation in Ionic Liquids.
Authors: Gieshoff Tim N. , Welther Alice , Kessler Michael T. , Prechtl Martin H. G. , Jacobi von Wangelin Axel 
Published: June 2014

AbstractIron nanoparticles in ionic liquids are shown to catalyze the semi‐hydrogenation of alkynes (I).
ChemInform Abstract: Palladium‐Catalyzed Cross‐Coupling Reaction of Trialkylbismuthines with 2‐Haloazines and Diazines.
Authors: Gagnon Alexandre , Petiot Pauline 
Published: June 2014

AbstractAn efficient method for the cross‐coupling of primary trialkylbismuth compounds with 2‐haloazines‐ and ‐diazines, that tolerates various functional groups is developed.
ChemInform Abstract: A Mild and Efficient Copper‐Catalyzed N‐Arylation of Unprotected Sulfonimidamides Using Boronic Acids.
Authors: Battula S. R. K. , Subbareddy G. V. , Chakravarthy I. E. 
Published: June 2014

AbstractNotable advantages of the novel method are a low‐cost catalyst, base‐free conditions, and good functional group tolerance.
ChemInform Abstract: Facile Synthesis of Hantzsch 1,4‐Dihydropyridines with Unsymmetrical 2,6‐ and 3,5‐Substituents.
Authors: Wang Zhen , Liu Qingjian , Zhang Wenwen , Chen Qing 
Published: June 2014

AbstractThe presented variation of the classical Hantzsch synthesis constitutes a facile one‐pot two step procedure.
ChemInform Abstract: Efficient Synthesis of Pyrrolo[1,2‐f]phenanthridine Derivatives via Dipolar Cycloaddition of Phenanthridine, Activated Acetylenes, and Ethyl Bromopyruvate.
Authors: Mehrabi Hossein , Pishahang Jaleh 
Published: June 2014

AbstractThe three‐component reaction of phenanthridine with activated acetylenes and bromopyruvate affords pyrrolophenanthridine regioisomers (IV) and (V) without the need of any catalyst.
ChemInform Abstract: Hydrosilylation Catalysis by an Earth Alkaline Metal Silyl: Synthesis, Characterization, and Reactivity of Bis(triphenylsilyl)calcium.
Authors: Leich Valeri , Spaniol Thomas P. , Maron Laurent , Okuda Jun 
Published: June 2014

AbstractBis(triphenylsilyl)calcium, obtained in high yield as a crystalline THF‐adduct, efficiently and regioselectively catalyzes the hydrosilylation of activated C—C double bonds.
ChemInform Abstract: Two New Pregnane Glycosides from the Roots of Cynanchum atratum
Authors: Wang Shilei , Shan Weiguang , Ma Liefeng , Zheng Zhenzhen , Ying Youmin , Zhan Zhajun 
Published: June 2014

Abstractisolation and structure elucidation of two new pregnane glycosides, cynatraosides A (I) and B (II), along with six known steroids
ChemInform Abstract: The First Total Synthesis of the Antiplasmodial Alkaloid (.+‐.)‐Cassiarin C Based on a Microwave‐Assisted Thermal Azaelectrocyclic Reaction.
Authors: Hibino Satoshi , Choshi Tominari , Tsuchiya Yuhta , Nishiyama Takashi , Hatae Noriyuki , Nemoto Hideo , Tazaki Yoshinari 
Published: June 2014

AbstractThe synthesis of alkaloid (I) is achieved in six steps in 60% overall yield from dihydroxymethylchromanone.
ChemInform Abstract: Novel Cytotoxic Metabolites from the Marine‐Derived Fungus Trichoderma citrinoviride.
Authors: Chen Li , Zhang Qi‐Qing , Hu Xiao , Gong Mei‐Wei , Zhang Wei‐Wei , Zheng Qiu‐Hong , Liu Qin‐Ying 
Published: June 2014

AbstractTrichoderiol C (I), citrinoviric acid (II), and penicillenol D (III) are isolated from T.