bluuebunny/crossref_metadata_2025_split huggingface dataset:
Imaging the condensation and evaporation of molecularly thin ethanol films with surface forces apparatus
Authors: Zhao Gutian , Tan Qiyan , Xiang Li , Zhang Di , Ni Zhonghua , Yi Hong , Chen Yunfei 
Published: January 2014

A new method for imaging condensation and evaporation of molecularly thin ethanol films is reported. It is found that the first adsorbed layer of ethanol film on mica surface behaves as solid like structure that cannot flow freely. With the increase of exposure time, more ethanol molecules condense over the mica surface in the saturated ethanol vapor condition. The first layer of adsorbed ethanol film is about 3.8 Å thick measured from the surface forces apparatus, which is believed to be the average diameter of ethanol molecules while they are confined in between two atomically smooth mica surfaces.
Chemo-informatic strategy for imaging mass spectrometry-based hyperspectral profiling of lipid signatures in colorectal cancer
Authors: Veselkov Kirill A. , Mirnezami Reza , Strittmatter Nicole , Goldin Robert D. , Kinross James , Speller Abigail V. M. , Abramov Tigran , Jones Emrys A. , Darzi Ara , Holmes Elaine , Nicholson Jeremy K. , Takats Zoltan 
Published: January 2014

SignificanceMass spectrometry imaging (MSI) technology represents a highly promising approach in cancer research. Here, we outline current roadblocks in translational MSI and introduce a comprehensive workflow designed to address current methodological limitations. An integrated bioinformatics platform is presented that allows intuitive histology-directed interrogation of MSI datasets. We show that this strategy permits the analysis of multivariate molecular signatures with direct correlation to morphological regions of interest, which can offer new insights into how different tumor microenvironmental populations interact with one another and generate novel region-of-interest specific biomarkers and therapeutic targets.
Redox-dependent stability, protonation, and reactivity of cysteine-bound heme proteins
Authors: Zhong Fangfang , Lisi George P. , Collins Daniel P. , Dawson John H. , Pletneva Ekaterina V. 
Published: January 2014

SignificanceMetal thiolates are common components of electron transfer proteins, enzymes, and redox sensors. Reduction of ferric thiolate hemes often involves protonation of the Cys ligand and ligand loss or substitution. These reactions contribute to enzyme inactivation and form the basis of sensing functions, but their mechanistic details are not well understood, particularly in proteins. A neutral thiol is a viable ligand to the heme; however, analysis of this interaction has been challenging. Herein, we describe a series of model proteins that offer detailed spectroscopic and thermodynamic characterization of ferric thiolate and ferrous thiol species as well as their interconversions. Results have shed light on the forces that control a particular heme ligation and redox reactivity of these key bioinorganic centers.
Recovering Individual Preferences for Non‐Market Goods: A Collective Travel‐Cost Model
Authors: Menon Martina , Perali Federico , Veronesi Marcella 
Published: January 2014

AbstractTraditional recreation demand models do not make a distinction between a household and an individual as the reference decision‐making unit, thus assuming that a family maximizes a single utility function, even if the family consists of different individuals. Such models ignore the possibility of family members' divergent preferences for non‐market goods. This study proposes a novel approach—the collective travel‐cost model (CTCM)—to eliciting individual preferences for a non‐market good, such as a recreation site, by using revealed preference data. This approach accounts for the intra‐household resource allocation and the role of each household member's preferences. We show that the collective travel‐cost model can be applied to estimating a recreation demand model that yields individual welfare estimates appropriate for policy analysis of non‐market goods, such as the willingness to pay to access a recreation site. We find that how resources are distributed within the household reflects significant differences in welfare measures.
De Novo Assembly and Characterization of the Transcriptome of the Parasitic Weed Dodder Identifies Genes Associated with Plant Parasitism
Authors: Ranjan Aashish , Ichihashi Yasunori , Farhi Moran , Zumstein Kristina , Townsley Brad , David-Schwartz Rakefet , Sinha Neelima R. 
Published: January 2014

Abstract
Parasitic flowering plants are one of the most destructive agricultural pests and have major impact on crop yields throughout the world. Being dependent on finding a host plant for growth, parasitic plants penetrate their host using specialized organs called haustoria. Haustoria establish vascular connections with the host, which enable the parasite to steal nutrients and water. The underlying molecular and developmental basis of parasitism by plants is largely unknown. In order to investigate the process of parasitism, RNAs from different stages (i.e. seed, seedling, vegetative strand, prehaustoria, haustoria, and flower) were used to de novo assemble and annotate the transcriptome of the obligate plant stem parasite dodder (Cuscuta pentagona). The assembled transcriptome was used to dissect transcriptional dynamics during dodder development and parasitism and identified key gene categories involved in the process of plant parasitism. Host plant infection is accompanied by increased expression of parasite genes underlying transport and transporter categories, response to stress and stimuli, as well as genes encoding enzymes involved in cell wall modifications. By contrast, expression of photosynthetic genes is decreased in the dodder infective stages compared with normal stem. In addition, genes relating to biosynthesis, transport, and response of phytohormones, such as auxin, gibberellins, and strigolactone, were differentially expressed in the dodder infective stages compared with stems and seedlings. This analysis sheds light on the transcriptional changes that accompany plant parasitism and will aid in identifying potential gene targets for use in controlling the infestation of crops by parasitic weeds.
The relationship between innovation capability and performance
Authors: Saunila Minna , Pekkola Sanna , Ukko Juhani 
Published: January 2014

Purpose
– The purpose of this paper is to discuss whether performance measurement moderates the relationship between innovation capability and firm performance.

Design/methodology/approach
– The study was conducted through a web-based survey in small- and medium-sized enterprises (SMEs). A total of 311 responses were received from a sample of 2,400 randomly selected SMEs.

Findings
– Consistent with predictions, the link between innovation capability and firm performance is significant in the presence of performance measurement. Performance measurement can thus be used as a tool for improving the performance of SMEs through innovation capability.

Practical implications
– Using the results of this study, practitioners can improve their innovation capability through performance measurement by taking better account of various aspects.

Originality/value
– Previous research has studied the effects of performance measurement, but not in the area of innovation management. Previous research has also showed that measurement has positive effects on innovation capability. The present study goes one step further by investigating the impacts of measurement on the relationship of multiple aspects of innovation capability and firm performance.
Antenatal interventions to reduce maternal distress: a systematic review and meta‐analysis of randomised trials
Authors: Fontein‐Kuipers YJ , Nieuwenhuijze MJ , Ausems M , Budé L , de Vries R 
Published: January 2014

BackgroundMaternal distress can have adverse health outcomes for mothers and their children. Antenatal interventions may reduce maternal distress.ObjectiveTo assess the effectiveness of antenatal interventions for the reduction of maternal distress during pregnancy and for up to 1 year postpartum.Search strategyEBSCO, Medline, PubMed, Cochrane, secondary references of Cochrane reviews and review articles, and experts in the field.Selection criteriaRandomised controlled trials in which the association between an antenatal intervention and the reduction of maternal distress was reported.Data collection and analysisTwo authors independently abstracted data from each trial. A random‐effects meta‐analysis assessed the reduction of maternal distress associated with antenatal preventive and treatment interventions, compared with routine antenatal care or another intervention.Main resultsTen trials with 3167 participants met the inclusion criteria, and nine trials (n = 3063) provided data for the meta‐analysis of six preventive interventions and three treatment interventions. The preventive interventions indicated no beneficial reduction of maternal distress (six trials;n = 2793; standardised mean difference,SMD–0.06; 95% confidence interval, 95% CI−0.14–0.01). The treatment interventions indicated a significant effect for the reduction of maternal distress (three trials;n = 270;SMD–0.29; 95% CI–0.54 to –0.04). A sample of women, selected retrospectively, who were more vulnerable for developing maternal distress showed a significant reduction of maternal distress after the interventions (three trials;n = 1410;SMD–0.25; 95% CI–0.37 to –0.14).Author's conclusionsPreventive antenatal interventions for maternal distress show no effect. Antenatal interventions for women who have maternal distress or are at risk for developing maternal distress are associated with a small reduction in maternal distress.
Tolerance to ethanol intoxication after chronic ethanol: role of GluN2A and PSD‐95
Authors: Daut Rachel A. , Busch Erica F. , Ihne Jessica , Fisher Daniel , Mishina Masayoshi , Grant Seth G. N. , Camp Marguerite , Holmes Andrew 
Published: January 2014

AbstractThe neural and genetic factors underlying chronic tolerance to alcohol are currently unclear. The GluN2A N‐methyl‐D‐aspartate receptors (NMDAR) subunit and the NMDAR‐anchoring protein PSD‐95 mediate acute alcohol intoxication and represent putative mechanisms mediating tolerance. We found that chronic intermittent ethanol exposure (CIE) did not produce tolerance [loss of righting reflex (LORR)] or withdrawal‐anxiety in C57BL/6J, GluN2A or PSD‐95 knockout mice assayed 2–3 days later. However, significant tolerance to LORR was evident 1 day after CIE in C57BL/6J and PSD‐95 knockouts, but absent in GluN2A knockouts. These data suggest a role for GluN2A in tolerance, extending evidence that human GluN2A gene variation is involved in alcohol dependence.
A semiparametric approach to simultaneous covariance estimation for bivariate sparse longitudinal data
Authors: Das Kiranmoy , Daniels Michael J. 
Published: January 2014

SummaryEstimation of the covariance structure for irregular sparse longitudinal data has been studied by many authors in recent years but typically using fully parametric specifications. In addition, when data are collected from several groups over time, it is known that assuming the same or completely different covariance matrices over groups can lead to loss of efficiency and/or bias. Nonparametric approaches have been proposed for estimating the covariance matrix for regular univariate longitudinal data by sharing information across the groups under study. For the irregular case, with longitudinal measurements that are bivariate or multivariate, modeling becomes more difficult. In this article, to model bivariate sparse longitudinal data from several groups, we propose a flexible covariance structure via a novel matrix stick‐breaking process for the residual covariance structure and a Dirichlet process mixture of normals for the random effects. Simulation studies are performed to investigate the effectiveness of the proposed approach over more traditional approaches. We also analyze a subset of Framingham Heart Study data to examine how the blood pressure trajectories and covariance structures differ for the patients from different BMI groups (high, medium, and low) at baseline.
Endothelial Nitric Oxide Synthase Protects Neurons against Ischemic Injury through Regulation of Brain‐Derived Neurotrophic Factor Expression
Authors: Li Shi‐Ting , Pan Jing , Hua Xu‐Ming , Liu Hong , Shen Sa , Liu Jia‐Fu , Li Bin , Tao Bang‐Bao , Ge Xiao‐Li , Wang Xu‐Hui , Shi Juan‐Hong , Wang Xiao‐Qiang 
Published: January 2014

SummaryAimsSeveral lines of evidence demonstrated that endothelial nitric oxide synthase (eNOS) confers protective effects during cerebral ischemia. In this study, we explored the underlying cellular and molecular mechanisms of neuroprotection by eNOS.MethodsA series of in vivo and in vitro ischemic models were employed to study the role of eNOS in maintaining neuronal survival and to identify the downstream factors.ResultsThe current data showed that pretreatment with a specific eNOS inhibitor, L‐N5‐(1‐iminoethyl) ornithine (L‐NIO), aggravated the neuronal loss in the rat cerebral ischemic model, accompanied by reduction in brain‐derived neurotrophic factor (BDNF) level, which was consistent with the findings in an oxygen‐glucose deprivation model (OGD) with two neuronal cells: primary rat cortical neurons and human neuroblastoma SH‐SY5Y cells. Furthermore, the extensive neuronal loss induced by L‐NIO was totally abolished by exogenous BDNF in both in vitro and in vivo models. On the other hand, eNOS overexpression through an adenoviral vector exerted a prominent protective effect on the neuronal cells subject to OGD, and the protective effect was totally abrogated by a neutralizing anti‐BDNF antibody.ConclusionCollectively, our results indicate that the neuroprotection of neuron‐derived eNOS against the cerebral ischemia was mediated through the regulation of BDNF secretion. In conclusion, our discovery provides a novel explanation for the neuroprotective effect of eNOS under pathological ischemic conditions such as stroke.
Postprandial effects of long‐term niacin/laropiprant use on glucose and lipid metabolism and on cardiovascular risk in patients with polycystic ovary syndrome
Authors: Aye M. M. , Kilpatrick E. S. , Afolabi P. , Wootton S. A. , Rigby A. S. , Coady A. M. , Sandeman D. D. , Atkin S. L. 
Published: January 2014

AimThis study investigated the effect of long‐term niacin/laropiprant therapy on CV risk and IR in obese women with PCOS.MethodsIn this double‐blind randomized placebo‐controlled trial, 13 and 12 PCOS women completed a 12 week course of niacin/laropiprant or placebo, respectively. Fasted subjects had an endothelial function test (EndoPat2000) and then consumed a mixed meal with blood sampled postprandially for 6 h before and after intervention.ResultsBy 12 weeks, niacin/laropiprant lowered low‐density lipoprotein cholesterol (LDL‐c) (13%) and increased HDL‐c (17%). Despite a reduction in fasting triglycerides (21%), the drug had no effect on their postprandial rise (2.69 ± 1.44 vs. 2.49 ± 1.14 mmol/l, p = 0.72). However, following the mixed meal, plasma glucose area under the response curve increased from 13.1 ± 2.9 to 14.0 ± 2.8 mmol/l, p = 0.05, as a consequence of both increased insulin resistance [HOMA‐IR: 2.2 (1.2, 4.2) vs. 3.8(1.3, 5.5), p = 0.02] and a reduced acute insulin response to glucose [424 (211, 975) vs. 257(122, 418) pmol/mmol, p = 0.04]. Niacin/laropiprant did not improve RHI (1.97 ± 0.40 vs. 2.05 ± 0.58, p = 0.33) or hsCRP.ConclusionsIn PCOS, niacin/laropiprant had a significant negative impact on postprandial glucose and no improvement in postprandial hypertriglyceridaemia, with at least the former mediated through increased IR and reduced β‐cell function. This data may help explain why the improvement in fasting lipids has not translated into improved CV risk markers in PCOS.
Using PowerPoint Animations to Teach Operations Management Techniques and Concepts
Authors: Treleven Mark D. , Penlesky Richard J. , Callarman Thomas E. , Watts Charles A. , Bragg Daniel J. 
Published: January 2014

ABSTRACTThis article examines the value of using complex animated PowerPoint presentations to teach operations management techniques and concepts. To provide context, literature covering the use of PowerPoint animations in business education is briefly reviewed. The specific animations employed in this study are identified and their expected benefits to students and faculty are discussed. Evidence of their perceived value to students and faculty is then presented.
In vitro bioassay methods for laboratory screening of novel mosquito repellents
Authors: Deng Wan , Zhu Na , Mo Jianchu 
Published: January 2014

AbstractRepellents are highly effective in preventing humans from being bitten by mosquitoes and other blood‐feeding insects. Here we provide an overview of the representative in vitro bioassays for the laboratory testing of these agents. Currently, potential repellents are mostly evaluated on human skin, using the arm‐in‐cage method. However, for the testing of large numbers of chemicals for mosquito repellent activity, several in vitro bioassays have been established. Based on their components, as described in the recent literature, these can be categorized: (i) the natural tendency system; (ii) the membrane plus blood system; (iii) the skin plus attractants system; and (iv) the host plus netting system. The major advantages and disadvantages of these bioassays are discussed with the aim of establishing reliable and practical methods for discovering novel mosquito repellents.
Efficacy of perampanel: A review of pooled data
Authors: Steinhoff Bernhard J. 
Published: January 2014

SummaryPerampanel (PER) has been tested in three randomized placebo‐controlled prospective phase III trials as an adjunctive antiepileptic drug (AED) in adult and adolescent patients age 12 years and older who had ongoing focal epileptic seizures despite receiving one to three AEDs. Patients were randomized to once‐daily placebo or maintenance dosages of 2, 4, or 8 mg of PER daily in one trial or to dosages of 8 and 12 mg of PER in the other two studies. Baseline and double‐blind titration periods comprised 6 weeks each before a 12‐week maintenance phase. Primary endpoints were median change in partial seizure frequency (baseline vs. double‐blind phase), and the percentage of patients achieving >50% reduction in seizure frequency (so‐called responders, baseline vs. maintenance). All patients had the opportunity to enter an open follow‐up study that allowed a titration of PER of up to 12 mg. Pooled data of all three studies included 1,478 patients. There was a statistically significant median change in seizure frequency and responder rates with PER dosages of 4, 8, and 12 mg (p < 0.01, each dose vs. placebo). Both the results of each trial and the pooled data are reported in this review. For the open follow‐up study, 1,218 patients were recruited. Ninety‐one percent finally reached maintenance dosages of 10 or 12 mg. After 1 year, both median seizure reduction and responder rates reached almost 50%.
Introduction: Perampanel—New mode of action and new option for patients with epilepsy
Authors: Steinhoff Bernhard J. 
Published: January 2014

SummaryWith perampanel the first‐in‐class selective alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolprepionic acid (AMPA) receptor antagonist has been finally approved for add‐on treatment in patients with focal‐onset seizures. This suggests additional therapeutic potential in hitherto difficult‐to‐treat epilepsies.
Soil animal responses to moisture availability are largely scale, not ecosystem dependent: insight from a cross‐site study
Authors: Sylvain Zachary A. , Wall Diana H. , Cherwin Karie L. , Peters Debra P. C. , Reichmann Lara G. , Sala Osvaldo E. 
Published: January 2014

AbstractClimate change will result in reduced soil water availability in much of the world either due to changes in precipitation or increased temperature and evapotranspiration. How communities of mites and nematodes may respond to changes in moisture availability is not well known, yet these organisms play important roles in decomposition and nutrient cycling processes. We determined how communities of these organisms respond to changes in moisture availability and whether common patterns occur along fine‐scale gradients of soil moisture within four individual ecosystem types (mesic, xeric and arid grasslands and a polar desert) located in the western United States and Antarctica, as well as across a cross‐ecosystem moisture gradient (CEMG) of all four ecosystems considered together. An elevation transect of three sampling plots was monitored within each ecosystem and soil samples were collected from these plots and from existing experimental precipitation manipulations within each ecosystem once in fall of 2009 and three times each in 2010 and 2011. Mites and nematodes were sorted to trophic groups and analyzed to determine community responses to changes in soil moisture availability. We found that while both mites and nematodes increased with available soil moisture across theCEMG, within individual ecosystems, increases in soil moisture resulted in decreases to nematode communities at all but the arid grassland ecosystem; mites showed no responses at any ecosystem. In addition, we found changes in proportional abundances of mite and nematode trophic groups as soil moisture increased within individual ecosystems, which may result in shifts within soil food webs with important consequences for ecosystem functioning. We suggest that communities of soil animals at local scales may respond predictably to changes in moisture availability regardless of ecosystem type but that additional factors, such as climate variability, vegetation composition, and soil properties may influence this relationship over larger scales.
Effects of comprehensive geriatric assessment‐based individually targeted interventions on mobility of pre‐frail and frail community‐dwelling older people
Authors: Tikkanen Päivi , Lönnroos Eija , Sipilä Sarianna , Nykänen Irma , Sulkava Raimo , Hartikainen Sirpa 
Published: January 2014

AimTo assess the effects of comprehensive geriatric assessment (CGA)‐based individually targeted interventions on the ability to walk 400 m in pre‐frail or frail and non‐frail community‐dwelling older people.MethodsA subgroups analysis of a population‐based comparative study, theGeriatricMultidisciplinaryStrategy for theGoodCare of theElderly (GeMS) was carried out in the city ofKuopio,Finland, from 2004 to 2007. This study was based on data from 2005 to 2007. The present analysis included 605 community‐dwelling older adults aged ≥76 years (mean age 80.9, 70% women), 314 in the intervention and 291 in the control group. Frailty status was assessed in 2005. Mobility was assessed by self‐reported ability to walk 400 m. The generalized estimating equation model with binary logistic regression was used to assess the treatment effect of the interventions on the ability to walk 400 m between 2005 and 2007.ResultsIn 2005, 55% (n = 173) of the participants in the intervention group and 64% (n = 187) in the control group were pre‐frail or frail. The intervention prevented the loss of ability to walk 400 m among pre‐frail and frail persons (OR0.74, 95%CI0.59–0.93,P = 0.01). The treatment effect was not statistically significant among non‐frail participants (OR0.99, 95%CI0.68–1.42,P = 0.94).ConclusionsCGA‐based individually targeted interventions were effective in preventing mobility limitations among pre‐frail and frail older people.Geriatr Gerontol Int 2015; 15: 80–88.