bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genotype to Phenotype Maps: Multiple Input Abiotic Signals Combine to Produce Growth Effects via Attenuating Signaling Interactions in Maize
Authors: Makumburage G Buddhika , Richbourg H Lee , LaTorre Kalindi D , Capps Andrew , Chen Cuixen , Stapleton Ann E 
Published: October 2013

Abstract
The complexity of allele interactions constrains crop improvement and the prediction of disease susceptibility. Additive allele effects are the foundation for selection in animal and plant breeding, and complex genetic and environmental interactions contribute to inefficient detection of desirable loci. Manipulation and modeling of other sources of variation, such as environmental variables, have the potential to improve our prediction of phenotype from genotype. As an example of our approach to analysis of the network linking environmental input to alleles, we mapped the genetic architecture of single and combined abiotic stress responses in two maize mapping populations and compared the observed genetic architecture patterns to simple theoretical predictions. Comparisons of single and combined stress effects on growth and biomass traits exhibit patterns of allele effects that suggest attenuating interactions among physiological signaling steps in drought and ultraviolet radiation stress responses. The presence of attenuating interactions implies that shared QTL found in sets of environments could be used to group environment types and identify underlying environmental similarities, and that patterns of stress-dependent genetic architecture should be studied as a way to prioritize prebreeding populations. A better understanding of whole-plant interactor pathways and genetic architecture of multiple-input environmental signaling has the potential to improve the prediction of genomic value in plant breeding and crop modeling.
Mated Progeny Production Is a Biomarker of Aging inCaenorhabditis elegans
Authors: Pickett Christopher L , Dietrich Nicholas , Chen Junfang , Xiong Chengjie , Kornfeld Kerry 
Published: October 2013

AbstractThe relationships between reproduction and aging are important for understanding the mechanisms of aging and evaluating evolutionary theories of aging. To investigate the effects of progeny production on reproductive and somatic aging, we conducted longitudinal studies of Caenorhabditis elegans hermaphrodites. For mated wild-type animals that were not sperm limited and survived past the end of the reproductive period, high levels of cross-progeny production were positively correlated with delayed reproductive and somatic aging. In this group of animals, individuals that generated more cross progeny also reproduced and lived longer than individuals that generated fewer cross progeny. These results indicate that progeny production does not accelerate reproductive or somatic aging. This longitudinal study demonstrated that cumulative cross progeny production through day four is an early-stage biomarker that is a positive predictor of longevity. Furthermore, in mated animals, high levels of early cross progeny production were positively correlated with high levels of late cross progeny production, indicating that early progeny production does not accelerate reproductive aging. The relationships between progeny production and aging were further evaluated by comparing self-fertile hermaphrodites that generated relatively few self progeny with mated hermaphrodites that generated many cross progeny. The timing of age-related somatic degeneration was similar in these groups, suggesting progeny production does not accelerate somatic aging. These studies rigorously define relationships between progeny production, reproductive aging, and somatic aging and identify new biomarkers of C. elegans aging. These results indicate that some mechanisms or pathways control age-related degeneration of both reproductive and somatic tissues in C. elegans.
Regulation of Gene Expression inNeurospora crassawith a Copper Responsive Promoter
Authors: Lamb Teresa M , Vickery Justin , Bell-Pedersen Deborah 
Published: October 2013

AbstractPrecise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled by copper availability. Excess copper represses, and copper depletion, via the use of a copper chelator, activates expression. The kinetics of induction and repression of tcu-1 are rapid, and the effects are long lived. We constructed a plasmid carrying the bar gene (for glufosinate selection) fused to the tcu-1 promoter. This plasmid permits the generation of DNA fragments that can direct integration of Ptcu-1 into any desired locus. We use this strategy to integrate Ptcu-1 in front of wc-1, a circadian oscillator and photoreceptor gene. The addition of excess copper to the Ptcu-1::wc-1 strain phenocopies a Δwc-1 strain, and the addition of the copper chelator, bathocuproinedisulfonic acid, phenocopies a wc-1 overexpression strain. To test whether copper repression can recapitulate the loss of viability that an essential gene knockout causes, we placed Ptcu-1 upstream of the essential gene, hpt-1. The addition of excess copper drastically reduced the growth rate as expected. Thus, this strategy will be useful to probe the biological function of any N. crassa gene through controlled expression.
Generalized Aggressive Periodontitis Associated With a Plasma Cell Gingivitis Lesion: A Case Report and Non‐Surgical Treatment
Authors: Parashis Andreas O. , Vardas Emmanouil , Tosios Konstantinos 
Published: October 2013

Introduction: Plasma cell gingivitis (PCG) is an unusual inflammatory condition characterized by dense, band‐like polyclonal plasmacytic infiltration of the lamina propria. Clinically, it appears as gingival enlargement with erythema and swelling of the attached and free gingiva and is not associated with any loss of attachment. The aim of this report is to present a rare case of severe generalized aggressive periodontitis (GAgP) associated with a PCG lesion that was successfully treated and maintained non‐surgically.Case Presentation: A 32‐year‐old white male with a non‐contributory medical history presented with gingival enlargement with diffuse erythema and edematous swelling, predominantly around teeth #5 through #8. Clinical and radiographic examination revealed generalized severe periodontal breakdown. A complete blood count and biochemical tests were within normal limits. Histologic and immunohistochemical examination were consistent with PCG. A diagnosis of severe GAgP associated with a PCG lesion was assigned. Treatment included elimination of possible allergens and non‐surgical periodontal treatment in combination with azithromycin. Clinical examination at reevaluation revealed complete resolution of gingival enlargement, erythema, and edema and localized residual probing depths of 5 mm. One year after treatment, the clinical condition was stable. Radiographs indicated improved bone levels and formation of crestal lamina dura.Conclusion: This case report highlights the unusual coexistence of GAgP and PCG, in which non‐surgical treatment with elimination of all possible causes in combination with antimicrobials resulted in elimination of the gingival enlargement and significant improvement of periodontal parameters.
Microbial Analysis of Subgingival Plaque Samples Compared to That of Whole Saliva in Patients With Periodontitis
Authors: Haririan Hady , Andrukhov Oleh , Bertl Kristina , Lettner Stefan , Kierstein Sonja , Moritz Andreas , Rausch‐Fan Xiaohui 
Published: October 2013

Background: The detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. The collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. However, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. The aim of this cross‐sectional study is to investigate whether there is a correlation between the results of different bacterial species in saliva and subgingival plaque samples from individuals with aggressive periodontitis (AgP) and chronic periodontitis (CP).Methods: Whole saliva and subgingival plaque samples from the deepest pocket of each quadrant were collected from 43 patients with CP and 33 patients with AgP. Twenty different bacterial species from both samplings were determined by the 16S ribosomal RNA‐based polymerase chain reaction with microarray technique.Results: All bacterial species were detected in salivary and subgingival plaque samples. For Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia, as well as Actinomyces viscosus, Campylobacter rectus/showae, Prevotella intermedia, Parvimonas micra, Eubacterium nodatum, and Campylobacter gracilis, a significant positive correlation between salivary and subgingival plaque samples was detected in patients with both types of periodontitis. There were no significant differences in bacteria in salivary and subgingival plaque samples between AgP and CP.Conclusion: Salivary analysis might be discussed as a potential alternative to subgingival plaque sampling for microbiologic analysis in both AgP and CP.
Analysis of Daytime Variations in Gingival Crevicular Fluid: A Circadian Periodicity?
Authors: Günday Sevim , Topcu Ali Orkun , Ercan Esra , Yamalik Nermin 
Published: October 2013

Background: Volumetric alterations in gingival crevicular fluid (GCF) are widely accepted to be associated with periodontal health/disease. The volume/flow of GCF was shown to be affected by an array of methodological factors. However, relatively limited information is available on whether GCF is subject to circadian rhythm. The main aim of the present study is to assess the possible presence/absence of GCF circadian rhythm. The impact of the sampling technique on daytime volumetric variations is also analyzed.Methods: The possible daily volumetric variations of GCF with 2‐hour intervals (from 08:00 to 18:00 hours) were assessed in 100 tooth sites and 600 GCF samples. Only maxillary incisors were included to eliminate any potential volumetric differences due to tooth dimensions. To analyze the potential impact of sampling technique on GCF volume and daytime variations, at one site modified intracrevicular sampling technique (MIST) was used, whereas the contralateral site was sampled with a deep intracrevicular sampling technique (DIST). Clinical periodontal parameters of the GCF sites were also recorded.Results: No significant daily variations in GCF volume could be detected. Higher volumetric measures were observed in inflamed subgroups compared with healthy subgroups (P <0.05). MIST was equivalent to DIST with regard to mean GCF volumes and the possible daytime volumetric alterations.Conclusions: Within the limits of the present study, it can be suggested that daytime variations did not have significant impact on GCF volume. The sampling methodology had no apparent impact on the circadian periodicity of GCF.
Primary dysmenorrhea is potentially predictive for initial orthodontic pain in female patients
Authors: Ye Rui , Wang Sheng , Li Yu , Wu Ruixian , Pei Jiao , Wang Jue , Zhao Zhihe 
Published: October 2013

ABSTRACT

Objective:
To investigate the relationship between primary dysmenorrhea (PD) and orthodontic pain in female patients, and to test the hypothesis that the intensity and duration of orthodontic pain could be roughly predicted by severity of PD.

Materials and Methods:
One hundred twenty college females were enrolled and put into one of three groups—mild (Mi), moderate (Mo), or severe (S)—according to level of menstrual pain. Intensity of the orthodontic pain was measured by visual analog scale (VAS) on days 1, 2, 4, 7, 14, and 28 after archwire placement.

Results:
As the intensity of orthodontic pain declined with time, the three groups demonstrated different changes during the initial week. Mi had the lowest VAS scores, whereas S possessed the highest scores. In contrast, Mo stayed in between. Significantly positive correlations were found between the severity of PD and the intensity of orthodontic pain at each time point within the first 2 weeks. In addition, though the majority of subjects reported disappearance of pain by the end of the second week in both Mi and Mo, a large proportion of females still perceived pain in S.

Conclusion:
Females with higher levels of menstrual pain tended to perceive orthodontic pain with higher intensity and more prolonged duration. Thus, PD could potentially serve as a reference to predict orthodontic pain in clinical settings.
Land Cover Change Assessment of Vaal Harts Irrigation Scheme using Multi-temporal Satellite Sata
Authors: Otieno Fredrick Ao , Ojo Olumuyiwa I , Ochieng George M. 
Published: October 2013

Abstract
Land cover change (LCC) is important to assess the land use/land cover changes with respect to the development activities like irrigation. The region selected for the study is Vaal Harts Irrigation Scheme (VHS) occupying an area of approximately 36, 325 hectares of irrigated land. The study was carried out using Land sat data of 1991, 2001, 2005 covering the area to assess the changes in land use/land cover for which supervised classification technique has been applied. The Normalized Difference Vegetation Index (NDVI) index was also done to assess vegetative change conditions during the period of investigation. By using the remote sensing images and with the support of GIS the spatial pattern of land use change of Vaal Harts Irrigation Scheme for 15 years was extracted and interpreted for the changes of scheme. Results showed that the spatial difference of land use change was obvious. The analysis reveals that 37.86% of additional land area has been brought under fallow land and thus less irrigation area (18.21%). There is an urgent need for management program to control the loss of irrigation land and therefore reclaim the damaged land in order to make the scheme more viable.
Trace Metals in Soils and Several Brassicaceae Plant Species from Serpentine Sites of Serbia
Authors: Tomović Gordana M. , Mihailović Nevena Lj. , Tumi Ahmed F. , Gajić Boško A. , Mišljenović Tomica D. , Niketić Marjan S. 
Published: October 2013

Abstract
Serpentine soils from 16 sample points in Serbia as well as the roots and shoots of eight Brassicaceae family species: Aethionema saxatile, Alyssum montanum, Alyssum repens, Cardamine plumieri, Erysimum linariifolium, Erysimum carniolicum, Isatis tinctoria, Rorippa lippizensis, were analyzed with regard to their concentrations of P, K, Fe, Ca, Mg, Ni, Zn, Mn, Cu, Cr, Cd, and Pb. Most of the soil samples were typical of ultramafic sites with low concentrations of P, K and Ca and high concentrations of Mg, Fe, Ni and Zn. Ca/Mg ratio was <1 in most soil samples and Brassicaceae plants. Only in A. montanum, A. repens, E. linariifolium and R. lippizensis was the Ca/Mg ratio >1. The levels of P, K, Fe and Zn were high, Mn and Cu occurred in low amounts, whereas Cr, Cd, Co and Pb were only traceable. In the roots and shoots of A. montanum and A. repens the measured concentrations of Ni were 657 mg kg-1 and 676 mg kg-1 respectively, which is the first instance that such high concentrations of Ni were detected in these two species.
Effect of Fertilization on ß-D-Glucan Content in Oat Grain (Avena Sativa L.)
Authors: Havrlentová Michaela , Hlinková Andrea , Žofajová Alžbeta , Kováčik Peter , Dvončová Daniela , Deáková Ľubomíra 
Published: October 2013

Abstract
β-D-glucan is a soluble component of dietary fibre localised in the cell walls of cereal grains, especially oat and barley. This homopolysaccharide presents a wide spectrum of health-beneficial effects in human beings, and its higher concentration in oats makes it an essential component for human nutrition. Genetic and environmental factors influence the content of β-D-glucan. Four oat varieties (two naked and two hulled) were grown in experimental fields at Vígľaš-Pstruša (Central Slovakia) in two consecutive years (2007 and 2008). The experiment included five fertilisation treatments with application of nitrogen (N) (as ammonium nitrate with dolomite) before sowing, and with selenium (Se) at the end of the tilling period (in the form of sodium selenate). A higher average content of β-D-glucan and test weight were observed in naked oats, Avenuda and Detvan, compared with hulled Vendelin and Zvolen. By contrast, higher yield and thousand grains weight were detected in hulled oats. Fertilisation with N + Se increased the content of β-D-glucan, but significantly only in hulled oat grains. The warmer and drier climate in the year 2007 did not influence the content of β-D-glucan in oats, but caused a significant increase in thousand grains weight and test weight in both oat varieties, as well as grain yield in naked oats.
In Vitro Development of Porcine Nuclear-Transferred Embryos Derived from Fibroblast Cells Analysed Cytometrically for Apoptosis Incidence and Accuracy of Cell Cycle Synchronization at the G0/G1 Stages / Rozwój In Vitro Klonalnych Zarodków Świni Zrekonstruowanych Z Jąder Komórkowych Fibroblastów Cytometrycznie Analizowanych Pod Kątem Częstotliwości Występowania Śmierci Apoptotycznej I Efektywności Synchronizacji Cyklu Mitotycznego W Fazach G0/G1
Authors: Samiec Marcin , Skrzyszowska Maria , Bochenek Michał 
Published: October 2013

Abstract The study was undertaken to examine whether various strategies, including contact inhibition and serum starvation, that were used for artificial synchronization of mitotic cycle of porcine fibroblast cell lines affect differently the distribution of cell cycle stage frequencies and the occurrence of apoptotic cell death in the analysed cell samples. In vitro cultured (contact-inhibited or serumstarved) somatic cells were subjected to flow cytometric diagnostics of mitotic cycle together with the detection of late-apoptotic cell fractions with hypodiploid number of nuclear DNA molecules. Moreover, impact of the methods applied to synchronize the cell division cycle of different types of nuclear donor fibroblast cells (adult cutaneous and foetal fibroblasts) on the preimplantation developmental outcomes of cloned pig embryos was investigated. The developmental capabilities of nuclear-transferred (NT) embryos that were reconstituted with contact-inhibited or serum-depleted adult cutaneous fibroblast cells to reach the morula and blastocyst stages remained at the levels of 169/278 (60.8%) and 76/278 (27.3%) or 121/265 (45.7%) and 46/265 (17.4%), respectively. The proportions of NT embryos originating from contact-inhibited or serum-deprived foetal fibroblast cells that completed their development to the morula and blastocyst stages were 223/296 (75.3%) and 108/296 (36.5%) or 165/261 (63.2%) and 67/261 (25.7%), respectively. In conclusion, the flow cytometric analysis of cultured porcine adult cutaneous and foetal fibroblast cells revealed the high efficiency of the artificial synchronization of mitotic cycle at the G0/G1 stages as a consequence of applying the methods of either contact inhibition or serum deprivation. For both types of fibroblast cells used to reconstruct the enucleated oocytes, the strategies that were utilized to synchronize the cell division cycle of nuclear donor cells considerably influenced the in vitro developmental abilities of NT pig embryos. Developmental competencies to reach the morula/blastocyst stages for cloned embryos that had been reconstructed with contact-inhibited or serum-starved foetal fibroblast cell nuclei were significantly higher than those for embryos that had been reconstructed with contact-inhibited or serum-starved adult cutaneous fibroblast cell nuclei.
Somatic Cell Level in Dairy Cows’ Milk During Extended Lactation / Poziom Komórek Somatycznych W Mleku Krów W Laktacji Przedłużonej
Authors: Salamończyk Ewa , Guliński Piotr 
Published: October 2013

Abstract Milk analysis was performed in Black-and-White cows with a different percentage of Holstein- Friesian (HF) genes, which calved in 1995-2008 and were included in the milk recording programme. The aim of the present study was to determine the somatic cell level in milk obtained during the extended lactation phase (from day 306 to the end of lactation) compared to the standard lactation. The average somatic cell count in the milk of cows in extended lactation was 409,000 in the standard lactation of 305 days, 542,000 in the extended lactation phase and 427,000 per mL in full lactation of 381 days. Somatic cell count in the milk produced during the extended lactation phase (from day 306 to the end of lactation) was significantly higher compared with the milk harvested during the 305-day lactation. The lowest average somatic cell score (SCS) per mL raw milk in the extension phase was determined in the milk of primiparous cows (3.83). The factors which significantly affected the somatic cell count in the milk of cows extending their standard lactation included parity (lactation) and average daily milk yields from day 306 until the end of lactation. The correlation coefficients calculated for the somatic cell score (SCS) in standard lactation and in extended lactation phase, within selected factors, indicate that there was a positive correlation for both the lactation groups (standard lactation and extension period) as well as lactation (parity) (rp = 0.23 and rp = 0.33; P<0.01). It was also demonstrated that somatic cell count was on the decrease with increasing daily milk performance during extended lactation (rp = -0.23; P<0.01).
The Effect of Dietary Camelina Sativa Oil on Quality of Broiler Chicken Meat / Wpływ Udziału Oleju Z Lnianki Siewnej (Camelina Sativa) W Dawkach Dla Kurcząt Rzeźnych Na Jakość Mięsa
Authors: Pietras Mariusz P. , Orczewska-Dudek Sylwia 
Published: October 2013

Abstract An experiment was conducted on 600 broiler chickens to determine the effect of using Camelina sativa oil as a dietary component on meat quality indicators. Broiler chickens were raised on litter under standard conditions of feeding and maintenance. In the second period of rearing, the control group (I) received a diet containing 6% rapeseed oil. Experimental groups were fed on a diet containing 3% rapeseed oil and 3% Camelina sativa oil (group II) and 6% camelina oil (group III). At the end of rearing 8 chickens from each group were slaughtered. A simplified analysis of the carcasses was conducted. Blood samples were taken to determine the content of total fat, triglycerides, total cholesterol and its fractions. Muscle samples were analysed for the content of dry matter, total protein and crude fat, fatty acid composition and malonic aldehyde (TBA). The meat was subjected to sensory evaluation. It was found that the introduction of Camelina sativa oil to the grower diet for broiler chickens does not have a negative effect on rearing parameters and carcass quality. What was observed was a tendency to reduce the proportion of abdominal fat in the carcass with increasing level of oil used in compound feed and increases in the total protein content of breast meat in group II receiving 3% of camelina oil. The addition of 6% of Camelina sativa oil to compound feed significantly decreased the content of total cholesterol and its fractions in the blood plasma of chickens in comparison to the other groups. The introduction of 3% and 6% of Camelina sativa oil to chicken diets enriched the breast meat in n-3 PUFA, mainly α-linolenic acid (ALA) and did not impair the flavour qualities of the cooked meat. The meat from chickens fed on a diet containing 6% of camelina oil was characterized by a greater increase of ALA.