bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Comprehensive Expression Analysis of Cancer Testis Antigens in Head and Neck Squamous Cell Carcinoma Revels MAGEA3/6 as a Marker for Recurrence
Authors: Zamunér Fernando Tadeu , Karia Bruno Takao Real , de Oliveira Cleyton Zanardo , Santos Carlos Roberto dos , Carvalho André Lopes , Vettore André Luiz 
Published: January 2015

Abstract
Despite significant advances in the treatment of head and neck squamous cell carcinoma (HNSCC), the survival rate has not changed in the last decades. Therefore, the development of novel therapeutic strategies is pursued. Cancer–testis antigens (CTA) are strong immunogenic proteins with a tumor-restricted expression pattern, and are considered ideal targets for tumor-specific immunotherapeutic approaches. In this study, using an in silico approach, we selected, among 139 previously described CTA, candidates to be evaluated in 89 HNSCC and 20 normal mucosa samples. SPANX-CD (71.9%), MAGEB2 (44.9%), MAGEA1 (44.9%), MAGEB6 (32.6%), and CXORF48 (27.0%) were found frequently expressed in HNSCC, and over 85% of the tumors expressed at least one of these five CTAs. The mRNA positivity of CXORF48, MAGEB6, and CRISP2 presented significant associations with recognized clinical features for poor outcome. Furthermore, MAGEA3/6 positivity was associated with significantly better disease-free survival (DFS, P = 0.014), and the expression of this antigen was shown to be an independent prognostic factor for tumor recurrence. In conclusion, one of five selected CTAs is expressed in at least 85% of the HNSCCs, suggesting a possible usage as target for immunotherapeutic approaches, and the mRNA-positivity for MAGEA3/6 is shown to be an independent marker for DFS. Mol Cancer Ther; 14(3); 828–34. ©2015 AACR.
The Seasonal Cycle of Lower-Tropospheric Gravity Wave Activity at Davis, Antarctica (69°S, 78°E)
Authors: Alexander Simon , Murphy Damian 
Published: January 2015

Abstract
A VHF wind-profiling radar located at Davis in coastal East Antarctica (69°S, 78°E) collected data from September 2009 to August 2011 in the lower troposphere. Gravity wave activity is quantified using the radar’s wind velocity variances. ERA-Interim and Antarctic Mesoscale Prediction System (AMPS) forecast output are used to understand the gravity wave activity in the context of the synoptic-scale meteorology and to identify the likely source of the observed gravity waves. The seasonal cycle of lower-tropospheric gravity wave activity (2.0–3.2-km altitude) obtained from the radar data for waves with ground-based periods of 16 min–12.8 h reveals a maximum in winter and a minimum in summer. The largest gravity wave activity corresponds in time to the presence of a surface depression centered north of Davis that directs strong northeasterly winds along the Antarctic coastline. Case studies indicate that these winds interact with an ice ridgeline located around 60 km northeast and upwind of Davis. This interaction between synoptic northeasterly winds and the ridgeline results in the formation of orographic gravity waves, which are observed in the Davis radar data as large wind velocity perturbations.
Fixed-Point Observation of Mixed Layer Evolution in the Seasonally Ice-Free Chukchi Sea: Turbulent Mixing due to Gale Winds and Internal Gravity Waves
Authors: Kawaguchi Yusuke , Nishino Shigeto , Inoue Jun 
Published: January 2015

AbstractA fixed-point observation using the R/V Mirai was conducted in the ice-free northern Chukchi Sea of the Arctic Ocean during September of 2013. During the program the authors performed repeated microstructure measurements to reveal the temporal evolution of the surface mixed layer and mixing processes in the upper water column. The shelf region was initially characterized by a distinct two-layer system comprising a warmer/fresher top layer and a colder/saltier bottom layer. During the two-week observation period, the top-layer water showed two types of mixing processes: near-surface turbulence due to strong wind forcing and subsurface mixing due to internal gravity waves. In the first week, when the top layer was stratified with fresh sea ice meltwater, turbulent energy related to internal waves propagated through the subsurface stratification, resulting in a mechanical overturning near the pycnocline, followed by enhanced mixing there. In the second week, gale winds directly stirred up the upper water and then established a deeper homogenous layer. The combination of internal wave mixing and wind-driven turbulence may contribute to releasing the oceanic heat into the atmosphere, consequently promoting the preconditioning of surface water freezing.
A successfully treated case of hypopituitarism complicated with hyperosmolar hyperglycaemic state and rhabdomyolysis
Authors: Wang Yi-Chen , Gao Li-Chao , Xu Hui , Qu Xiao-Zhang , Wang Ye , Lou Xiao-Qian , Guo Hui 
Published: January 2015

Introduction This study aimed to report a rare case of hypopituitarism complicated with hyperosmolar hyperglycaemic state and rhabdomyolysis. Case presentation Hypopituitarism is a clinical syndrome in which there is a deficiency in hormone production by the pituitary gland. It often leads to hypoglycaemia, but in this case the patient was complicated with hyperosmolar hyperglycaemic state. The patient received prompt medical treatment, which effectively prevented the occurrence of possible acute kidney failure and other complications. Conclusion This is a complicated and rare case. Our report provides some indications for the timely diagnosis and the standardised treatments for a patient who has hypopituitarism complicated with hyperosmolar hyperglycaemic state and rhabdomyolysis.
The misuse and overuse of non-sterile gloves: application of an audit tool to define the problem
Authors: Wilson Jennie , Prieto Jacqui , Singleton Julie , O’Connor Vivienne , Lynam Siobhan , Loveday Heather 
Published: January 2015

Background: The use of non-sterile gloves (NSG) has become routine in the delivery of health care, often for procedures for which they are not required; their use may increase the risk of cross contamination and is generally not integrated into hand hygiene audit. This paper describes a small-scale application and validation of an observational audit tool devised to identify inappropriate use of NSG and potential for cross contamination. Methods: Two observers simultaneously observed the use of NSG during episodes of care in an acute hospital setting. The inter-rater reliability (IRR) of the audit tool was measured corrected for chance agreement using Kappa. Results: A total of 22 episodes of care using NSG were observed. In 68.6% (24/35) of procedures there was no contact with blood/body fluid; in 54.3% (19/35) NSG-use was inappropriate. The IRR was 100% for eight of 12 components of the tool. For hand hygiene before and after NSG removal it was 82% (Kappa = 0.72) and 95% (Kappa = 0.87). Conclusions: In this small-scale application of a glove-use audit tool we demonstrated over-use and misuse of NSG and potential for cross transmission on gloved hands. The audit tool provides an effective mechanism for integrating glove use into the audit of hand hygiene behaviour.
A genetic study on C5-TRAF1 and progression of joint damage in rheumatoid arthritis
Authors: van Steenbergen Hanna W , Rodríguez-Rodríguez Luis , Berglin Ewa , Zhernakova Alexandra , Knevel Rachel , Ivorra-Cortés Jose , Huizinga Tom WJ , Fernández-Gutiérrez Benjamin , Gregersen Peter K , Rantapää-Dahlqvist Solbritt , van der Helm-van Mil Annette HM 
Published: January 2015

Abstract
Introduction
The severity of joint damage progression in rheumatoid arthritis (RA) is heritable. Several genetic variants have been identified, but together explain only part of the total genetic effect. Variants in Interleukin-6 (IL-6), Interleukin-10 (IL-10), C5-TRAF1, and Fc-receptor-like-3 (FCRL3) have been described to associate with radiographic progression, but results of different studies were incongruent. We aimed to clarify associations of these variants with radiographic progression by evaluating six independent cohorts.

Methods
In total 5,895 sets of radiographs of 2,493 RA-patients included in six different independent datasets from the Netherlands, Sweden, Spain and North-America were studied in relation to rs1800795 (IL-6), rs1800896 (IL-10), rs2900180 (C5-TRAF1) and rs7528684 (FCRL3). Associations were tested in the total RA-populations and in anti-citrullinated peptide antibodies (ACPA)-positive and ACPA-negative subgroups per cohort, followed by meta-analyses. Furthermore, the associated region C5-TRAF1 was fine-mapped in the ACPA-negative Dutch RA-patients.

Results
No associations were found for rs1800795 (IL-6), rs1800896 (IL-10) and rs7528684 (FCRL3) in the total RA-population and after stratification for ACPA. Rs2900180 in C5-TRAF1 was associated with radiographic progression in the ACPA-negative population (P-value meta-analysis = 5.85 × 10−7); the minor allele was associated with more radiographic progression. Fine-mapping revealed a region of 66Kb that was associated; the lowest P-value was for rs7021880 in TRAF1. The P-value for rs7021880 in meta-analysis was 6.35 × 10−8. Previous studies indicate that the region of rs7021880 was associated with RNA expression of TRAF1 and C5.

Conclusion
Variants in IL-6, IL-10 and FCRL3 were not associated with radiographic progression. Rs2900180 in C5-TRAF1 and linked variants in a 66Kb region were associated with radiographic progression in ACPA-negative RA.
Low-temperature-operative Hydrogen Gas Sensor Employing 10 wt % Pt/Ce0.68Zr0.17Sn0.15O2.0 Catalyst
Authors: Hosoya Ayaka , Yasuhara Syun , Tamura Shinji , Imanaka Nobuhito 
Published: January 2015

Abstract
A novel catalytic combustion-type hydrogen gas sensor that could operate at low temperatures was devised by employing a Pt-loaded cerium oxide–zirconium oxide–tin oxide solid solution as the hydrogen oxidizing catalyst. Since the present catalyst showed high oxidizing activity toward hydrogen even at 40 °C, stable and quantitative H2 detection was achieved at as low as 40 °C with high selectivity.
Total Synthesis of a Monomeric Phloroglucinol Derivative Isolated from Myrtus communis
Authors: Nishimura Eiji , Ohfune Yasufumi , Shinada Tetsuro 
Published: January 2015

Abstract
The first total synthesis of a monomeric phloroglucinol [(1R*,2S*)-2-hydroxy-2-isobutyl-4,4,6,6-tetramethyl-3,5-dioxocyclohexyl acetate] was achieved by stereoselective reduction of a symmetrical α-ketol as a key step. The corresponding cis-stereoisomer of the natural product was synthesized from phloroglucinol via cis-dihydroxylation using OsO4. Comparison of 1H NMR data for synthetic 1 and 2 confirmed the trans-stereochemistry of the natural product.
Is tantalum protective against infection in revision total hip arthroplasty?
Authors: Tokarski A. T. , Novack T. A. , Parvizi J. 
Published: January 2015

We hypothesised that the use of tantalum (Ta) acetabular components in revision total hip arthroplasty (THA) was protective against subsequent failure due to infection. We identified 966 patients (421 men, 545 women and 990 hips) who had undergone revision THA between 2000 and 2013. The mean follow up was 40.2 months (3 months to 13.1 years). The mean age of the men and women was 62.3 years (31 to 90) and 65.1 years (25 to 92), respectively. Titanium (Ti) acetabular components were used in 536 hips while Ta components were used in 454 hips. In total, 73 (7.3%) hips experienced subsequent acetabular failure. The incidence of failure was lower in the Ta group at 4.4% (20/454) compared with 9.9% (53/536) in the Ti group (p < 0.001, odds ratio 2.38; 95% CI 1.37 to 4.27). Among the 144 hips (64 Ta, 80 Ti) for which revision had been performed because of infection, failure due to a subsequent infection was lower in the Ta group at 3.1% (2/64) compared with 17.5% (14/80) for the Ti group (p = 0.006). Thus, the use of Ta acetabular components during revision THA was associated with a lower incidence of failure from all causes and Ta components were associated with a lower incidence of subsequent infection when used in patients with periprosthetic joint infection. Cite this article: Bone Joint J 2015;97-B:45–9.
The incidence of stress fracture following peri-acetabular osteotomy
Authors: Malviya A. , Dandachli W. , Beech Z. , Bankes M. J. , Witt J. D. 
Published: January 2015

Stress fractures occurring in the pubis and ischium after peri-acetabular osteotomy (PAO) are not well recognised, with a reported incidence of 2% to 3%. The purpose of this study was to analyse the incidence of stress fracture after Bernese PAO under the care of two high-volume surgeons. The study included 359 patients (48 men, 311 women) operated on at a mean age of 31.1 years (15 to 56), with a mean follow-up of 26 months (6 to 64). Complete follow-up radiographs were available for 348 patients, 64 of whom (18.4%) developed a stress fracture of the inferior pubic ramus, which was noted at a mean of 9.1 weeks (5 to 55) after surgery. Most (58; 91%) healed. In 40 of the patients with a stress fracture (62.5%), pubic nonunion also occurred. Those with a stress fracture were significantly older (mean 33.9 years (16 to 50) vs 30.5 years (15 to 56), p = 0.002) and had significantly more mean pre-operative deformity: mean centre–edge angle (9.8° (-9.5 to 35) vs 12.4° (-33 to 28), p = 0.04) and mean Tönnis angle (22.8° (0 to 45) vs 18.7° (-2 to 38), p < 0.001). The pubic nonunion rate was significantly higher in those with a stress fracture (62.5% vs 7%, p < 0.001), with regression analysis revealing that these patients had 11.8 times higher risk than those without nonunion. Cite this article: Bone Joint J 2015; 97-B:24–8.
Outcome of one-stage correction of deformities of the ankle and hindfoot and fusion in Charcot neuroarthropathy using a retrograde intramedullary hindfoot arthrodesis nail
Authors: Siebachmeyer M. , Boddu K. , Bilal A. , Hester T. W. , Hardwick T. , Fox T. P. , Edmonds M. , Kavarthapu V. 
Published: January 2015

We report the outcomes of 20 patients (12 men, 8 women, 21 feet) with Charcot neuro-arthropathy who underwent correction of deformities of the ankle and hindfoot using retrograde intramedullary nail arthrodesis. The mean age of the patients was 62.6 years (46 to 83); their mean BMI was 32.7 (15 to 47) and their median American Society of Anaesthetists score was 3 (2 to 4). All presented with severe deformities and 15 had chronic ulceration. All were treated with reconstructive surgery and seven underwent simultaneous midfoot fusion using a bolt, locking plate or a combination of both. At a mean follow-up of 26 months (8 to 54), limb salvage was achieved in all patients and 12 patients (80%) with ulceration achieved healing and all but one patient regained independent mobilisation. There was failure of fixation with a broken nail requiring revision surgery in one patient. Migration of distal locking screws occurred only when standard screws had been used but not with hydroxyapatite-coated screws. The mean American Academy of Orthopaedic Surgeons Foot and Ankle (AAOS-FAO) score improved from 50.7 (17 to 88) to 65.2 (22 to 88), (p = 0.015). The mean Short Form (SF)-36 Health Survey Physical Component Score improved from 25.2 (16.4 to 42.8) to 29.8 (17.7 to 44.2), (p = 0.003) and the mean Euroqol EQ‑5D‑5L score improved from 0.63 (0.51 to 0.78) to 0.67 (0.57 to 0.84), (p = 0.012). Single-stage correction of deformity using an intramedullary hindfoot arthrodesis nail is a good form of treatment for patients with severe Charcot hindfoot deformity, ulceration and instability provided a multidisciplinary care plan is delivered. Cite this article: Bone Joint J 2015;97-B:76–82.
Pathologic role of neuronal nicotinic acetylcholine receptors in epileptic disorders: implication for pharmacological interventions
Authors: Ghasemi Mehdi , Hadipour-Niktarash Arash 
Published: January 2015

AbstractAccumulating evidence suggests that neuronal nicotinic acetylcholine receptors (nAChRs) may play a key role in the pathophysiology of some neurological diseases such as epilepsy. Based on genetic studies in patients with epileptic disorders worldwide and animal models of seizure, it has been demonstrated that nAChR activity is altered in some specific types of epilepsy, including autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE) and juvenile myoclonic epilepsy (JME). Neuronal nAChR antagonists also have antiepileptic effects in pre-clinical studies. There is some evidence that conventional antiepileptic drugs may affect neuronal nAChR function. In this review, we re-examine the evidence for the involvement of nAChRs in the pathophysiology of some epileptic disorders, especially ADNFLE and JME, and provide an overview of nAChR antagonists that have been evaluated in animal models of seizure.
Effect of Axial Velocity Density Ratio on the Performance of a Controlled Diffusion Airfoil Compressor Cascade
Authors: Senthil Kumaran R. , Kamble Sachin , Swamy K.M.M. , Nagpurwala Q.H. , Bhat Ananthesha 
Published: January 2015

AbstractAxial Velocity Density Ratio (AVDR) is an important parameter to check the two-dimensionality of cascade flows. It can have significant influence on the cascade performance and the secondary flow structure. In the present study, the effect of AVDR has been investigated on a highly loaded Controlled Diffusion airfoil compressor cascade. Detailed 3D Computational Fluid Dynamics (CFD) studies were carried out with the cascade at five different AVDRs. Key aerodynamic performance parameters and flow structure through the cascade were analyzed in detail. CFD results of one AVDR were validated with the experimental cascade test data and were seen to be in good agreement. Loss characteristics of the cascade varied significantly with change in AVDR. Increase in AVDR postponed the point of separation on the suction surface, produced thinner boundary layers and caused substantial drop in the pressure loss coefficient. Strong end wall vortices were noticed at AVDR of 1.177. At higher AVDRs, the flow was well guided even close to the end wall and the secondary flows diminished. The loading initially improved with increase in AVDR. Beyond a certain limit, further increase in AVDR offered no improvements to the loading but rather resulted in drop in diffusion and deviation.
Quality Control of Genotypes Using Heritability Estimates of Gene Content at the Marker
Authors: Forneris Natalia S , Legarra Andres , Vitezica Zulma G , Tsuruta Shogo , Aguilar Ignacio , Misztal Ignacy , Cantet Rodolfo J C 
Published: January 2015

Abstract
Quality control filtering of single-nucleotide polymorphisms (SNPs) is a key step when analyzing genomic data. Here we present a practical method to identify low-quality SNPs, meaning markers whose genotypes are wrongly assigned for a large proportion of individuals, by estimating the heritability of gene content at each marker, where gene content is the number of copies of a particular reference allele in a genotype of an animal (0, 1, or 2). If there is no mutation at the marker, gene content has an additive heritability of 1 by construction. The method uses restricted maximum likelihood (REML) to estimate heritability of gene content at each SNP and also builds a likelihood-ratio test statistic to test for zero error variance in genotyping. As a by-product, estimates of the allele frequencies of markers at the base population are obtained. Using simulated data with 10% permutation error (4% actual error) in genotyping, the method had a specificity of 0.96 (4% of correct markers are rejected) and a sensitivity of 0.99 (1% of wrong markers are accepted) if markers with heritability lower than 0.975 are discarded. Checking of Mendelian errors resulted in a lower sensitivity (0.84) for the same simulation. The proposed method is further illustrated with a real data set with genotypes from 3534 animals genotyped for 50,433 markers from the Illumina PorcineSNP60 chip and a pedigree of 6473 individuals; those markers underwent very little quality control. A total of 4099 markers with P-values lower than 0.01 were discarded based on our method, with associated estimates of heritability as low as 0.12. Contrary to other techniques, our method uses all information in the population simultaneously, can be used in any population with markers and pedigree recordings, and is simple to implement using standard software for REML estimation. Scripts for its use are provided.
IDENTIFICATION OF VOLATILE FLAVOUR COMPOUNDS OF HOKI (Macruronus novaezelandiae) AND ORANGE ROUGHY (Hoplostethus atlanticus) OILS
Authors: Irianto Hari Eko , Fernandez Carmen C. , Shaw G. J. 
Published: January 2015

Identification of volatile flavour compounds of hoki (Macruronus novaezelandiae) and orange roughy (Hoplostethus atlanticus) oils has been carried out. Flavour compounds were extracted by a purging system and collected using a porous polymer Tenax TA trap. The gas chromatography-mass spectrometry (GC-MS) was used to identify the volatile flavour compounds. The predominant compounds contributing to the volatile flavour of hoki oil were methyl ethyl benzoate, ethyl benzoate and 1,1-dimethylethyl-2-propionic acid. Meanwhile, the main volatile flavour components of orange roughy oil were toluene, cyclohexane, 1,1-dimethylethyl-2-methyl propionic acid and tetrachloroethane.
EFFECT OF SLAUGHTERING TECHNIQUES ON THE QUALITY OF FRESH “PATIN SIAM “ CATFISH (Pangasius hypopthalmus) STORED AT AMBIENT TEMPERATURE
Authors: Ikasari Diah , Suryaningrum Theresia Dwi 
Published: January 2015

Fish handling is one of factors that affect fish quality deterioration, including slaughtering technique. In this study, the quality deterioration of fresh patin Siam catfish (Pangasius hypopthalmus) which were handled with different slaughtering techniques was observed. Fish were fasted for one day before slaughtered with two different techniques namely live-chilling and bleeding techniques. Live-chilling technique was conducted by exposing fish into cold water with temperature of 0-5°C for 15 minutes, while bleeding technique was conducted by cutting directly on the arteries part of fish head. After being slaughtered, fish were then stored at ambient temperatures for 18 hours and observed for its quality deterioration in every 3 hours. The observed parameters of fish quality deterioration were including the sensory attributes which were described using descriptive test by trained panelists; chemical parameters including proximate analysis; pH and Total Volatile Base (TVB), and microbiological parameters including Total Plate Count (TPC), coliform and E.coli. Results showed that the flesh of patin Siam catfish slaughtered by live-chilling technique had more blood in the fish body tissue, while bleeding technique resulted in much brighter flesh as well as abdominal cavity. The flesh quality decreased after 18 hours of storage in both treatments, but no significant changes in their proximate values. Bleeding technique was more recommended compared to live-chilling technique for slaughtering patin Siam catfish due to its predominance in producing better flesh quality.
SEA CUCUMBER AS ANTICANCER AGENTS AND ITS DEVELOPMENT FOR FUNCTIONAL FOOD PRODUCTS
Authors: Ridhowati Sherly , Zakaria Fransiska Rungkat , Syah Dahrul , Chasanah Ekowati 
Published: January 2015

Indonesia is popularly known as sea cucumber (teripang) exporter in the form of dried teripang. Commonly known as beche-de-mer or gamat, sea cucumber has long been used as medicine and food by Asian and Middle East people. Recent study has shown that sea cucumbers contain active compounds that show potential health benefits and other biological properties such as antibacterial and antifungal products, anticoagulants, antihypertensives, immuno modulation, inhibitor of osteoclastogenesis. It was reported that sea cucumber posses aphrodisiacs, potentially improve immunity, anticancer and anticoagulation. Sea cucumber is also rich in collagen as a component of connective tissue which can further be converted into smaller molecule and act as bioactive substances. This review presents the potential of sea cucumber as a functional food especially to prevent cancer and strategy to develop sea cucumber-based functional food by enzymatic hydrolysis and in vivo study.