bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel radiation-induced p53-dependent transcripts extensively regulated during mouse brain development
Authors: Quintens Roel , Verreet Tine , Janssen Ann , Neefs Mieke , Leysen Liselotte , Michaux Arlette , Verslegers Mieke , Samari Nada , Pani Giuseppe , Verheyde Joris , Baatout Sarah , Benotmane Mohammed A. 
Published: February 2015

ABSTRACT
Ionizing radiation is a potent activator of the tumor suppressor gene p53, which itself regulates the transcription of genes involved in canonical pathways such as the cell cycle, DNA repair and apoptosis as well as other biological processes like metabolism, autophagy, differentiation and development. In this study, we performed a meta-analysis on gene expression data from different in vivo and in vitro experiments to identify a signature of early radiation-responsive genes which were predicted to be predominantly regulated by p53. Moreover, we found that several genes expressed different transcript isoforms after irradiation in a p53-dependent manner. Among this gene signature, we identified novel p53 targets, some of which have not yet been functionally characterized. Surprisingly, in contrast to genes from the canonical p53-regulated pathways, our gene signature was found to be highly enriched during embryonic and post-natal brain development and during in vitro neuronal differentiation. Furthermore, we could show that for a number of genes, radiation-responsive transcript variants were upregulated during development and differentiation, while radiation non-responsive variants were not. This suggests that radiation exposure of the developing brain and immature cortical neurons results in the p53-mediated activation of a neuronal differentiation program. Overall, our results further increase the knowledge of the radiation-induced p53 network of the embryonic brain and provide more evidence concerning the importance of p53 and its transcriptional targets during mouse brain development.
A novel GAA repeat expansion-based mouse model of Friedreich ataxia
Authors: Anjomani Virmouni Sara , Ezzatizadeh Vahid , Sandi Chiranjeevi , Sandi Madhavi , Al-Mahdawi Sahar , Chutake Yogesh , Pook Mark A. 
Published: February 2015

Abstract
Friedreich ataxia (FRDA) is an autosomal recessive neurodegenerative disorder caused by a GAA repeat expansion mutation within intron 1 of the FXN gene, resulting in reduced levels of frataxin protein. We have previously reported the generation of human FXN yeast artificial chromosome (YAC) transgenic FRDA mouse models containing 90-190 GAA repeats, but the presence of multiple GAA repeats within these mice is considered suboptimal. We now describe the cellular, molecular and behavioural characterisation of a newly developed YAC transgenic FRDA mouse model, designated YG8sR, which we have shown by DNA sequencing to contain a single pure GAA repeat expansion. The founder YG8sR mouse contained 120 GAA repeats, but due to intergenerational expansion we have now established a colony of YG8sR mice that contain approximately 200 GAA repeats. We show that YG8sR mice have a single copy of the FXN transgene, which is integrated at a single site as confirmed by fluorescence in situ hybridisation (FISH) analysis of metaphase and interphase chromosomes. We have identified significant behavioural deficits, together with a degree of glucose intolerance and insulin hypersensitivity, in YG8sR FRDA mice compared to Y47R and wild-type (WT) control mice. We have also detected increased somatic GAA repeat instability in the brain and cerebellum of YG8sR mice, together with significantly reduced expression of FXN, FAST-1 and frataxin and reduced aconitase activity compared to Y47R mice. Furthermore, we have confirmed the presence of pathological vacuoles within neurons of the dorsal root ganglia (DRG) of YG8sR mice. These novel GAA repeat expansion-based YAC transgenic FRDA mice, which exhibit progressive FRDA-like pathology, represent an excellent model for the investigation of FRDA disease mechanisms and therapy.
Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling
Authors: Fellgett Simon W. , Maguire Richard J. , Pownall Mary Elizabeth 
Published: February 2015

ABSTRACT
Wnt signalling plays essential roles during embryonic development and is known to be mis-regulated in human disease. There are many molecular mechanisms that ensure tight regulation of Wnt activity. One such regulator is the heparan-sulfate-specific 6-O-endosulfatase Sulf1. Sulf1 acts extracellularly to modify the structure of heparan sulfate chains to affect the bio-availability of Wnt ligands. Sulf1 could, therefore, influence the formation of Wnt signalling complexes to modulate the activation of both canonical and non-canonical pathways. In this study, we use well-established assays in Xenopus to investigate the ability of Sulf1 to modify canonical and non-canonical Wnt signalling. In addition, we model the ability of Sulf1 to influence morphogen gradients using fluorescently tagged Wnt ligands in ectodermal explants. We show that Sulf1 overexpression has ligand-specific effects on Wnt signalling: it affects membrane accumulation and extracellular levels of tagged Wnt8a and Wnt11b ligands differently, and inhibits the activity of canonical Wnt8a but enhances the activity of non-canonical Wnt11b.
Laser-Induced Carbonization of Sumanene Derivatives: Exposure-Time Dependence of Time-Resolved Microwave Conductivity
Authors: Inada Yuhi , Amaya Toru , Saeki Akinori , Seki Shu , Hirao Toshikazu 
Published: February 2015

Abstract
Laser-induced carbonization of fullerene C60, sumanene derivatives, and triphenylene was studied from a view point of exposure-time dependence of time-resolved microwave conductivity (TRMC). Their increased rate of TRMC decreased in the order above.
Photophysical Properties of Chlorophyll Derivatives Linked with Rhenium Bipyridine Complexes
Authors: Kitagawa Yuichi , Ogasawara Shin , Kosumi Daisuke , Hashimoto Hideki , Tamiaki Hitoshi 
Published: February 2015

Abstract
Since photochemical CO2 reduction using long wavelength regions of sunlight is important, we have reported the first photochemical CO2 reduction with red visible light (&gt;650 nm) by using a synthetic chlorophyll derivative covalently linked with a rhenium bipyridine complex. In the reported system, electron/energy transfer from the photoexcited chlorophyll to the catalytic Re complex was not so effective. To accelerate the process for achieving efficient photochemical reduction of CO2, we controlled the redox potential of Re complex and improved the chlorophyll donor by a simple modification. As a result, we achieved strong quenching of the excited states of a 131-deoxochlorophyll derivative with a rhenium bipyridine complex in a molecule.
Phylogeny of the Inuleae (Asteraceae) with special emphasis on the Inuleae‐Plucheinae
Authors: Nylinder Stephan , Anderberg Arne A. 
Published: February 2015

AbstractPhylogenetic relationships in the tribe Inuleae (Asteraceae, sunflower family) are elucidated based on a concatenated set of nuclear (ETS, ITS), and chloroplast data (ndhF, trnL‐F, trnH‐psbA), analysed by Bayesian and parsimony methods. Extensive sampling of representatives from both subtribes Inuleae‐Inulinae and Inuleae‐Plucheinae establish their reciprocal monophyly, and result in the first‐ever resolved molecular phylogeny of the Inuleae‐Plucheinae with new insights into the relationships and morphological character distributions between genera and among species. Of the 31 accepted genera in the Inuleae‐Plucheinae, only Pseudoblepharispermum is not represented in this study, 12 monotypic genera are placed in the phylogeny, 13 genera are shown to be monophyletic, and only 5 of the remaining 18 genera are revealed to be polyphyletic. The implications for the nomenclature status of the monotypic and polyphyletic genera are discussed, together with a descriptive review of morphological characters traditionally used to circumscribe the genera in this subtribe.
Professional Capital as Accountability
Authors: Fullan Michael , Rincón-Gallardo Santiago , Hargreaves Andy 
Published: February 2015

This paper seeks to clarify and spells out the responsibilities of policy makers to create the conditions for an effective accountability system that produces substantial improvements in student learning, strengthens the teaching profession, and provides transparency of results to the public. The authors point out that U.S. policy makers will need to make a major shift from a heavy reliance on external accountability and superficial structural solutions (e.g., professional standards of practice) to investing in and building the professional capital of all teachers and leaders throughout the system. The article draws key lessons from highly effective school systems in the United States and internationally to argue that the priority for policy makers should be to lead with creating the conditions for internal accountability, that is, the collective responsibility within the teaching profession for the continuous improvement and success of all students. This approach is based on the development and circulation of professional capital that consists of three components: individual human capital, social capital (where teachers learn from each other), and decisional capital (developing judgment and expertise over time). In this new professional accountability model, the external accountability that reassures the public that the system is performing in line with societal expectations continues to be an important role of educational systems, but it is nurtured and sustained by the development of strong internal accountability.
Professional Capacity and Accountability: An Introduction
Authors: Darling-Hammond Linda , Snyder Jon 
Published: February 2015

This special issue furthers the conversation begun in the August 2014 of edition of Education Policy Analysis Archives in the article Accountability for College and Career Readiness: Developing a New Paradigm by Linda Darling-Hammond, Gene Wilhoit, and Linda Pittenger. That paper posits that as schools across the country take on the challenge of preparing all children for success in college, career, and life, states must in turn move toward creating more aligned systems of assessment and accountability. The authors recommend, “an accountability approach that focuses on meaningful learning, enabled by professionally skilled and committed educators, and supported by adequate and appropriate resources, so that all students regardless of background are prepared for both college and career when they graduate from high school” (p. 1). In this, the second of three focused volumes of EPAA, we hope to further that discussion and debate by focusing, one issue at a time, on each of the three elements of a truly responsible accountability system: 1) meaningful learning, 2) professional capacity and accountability, and 3) equitable and wisely used resources.
STAMP2 increases oxidative stress and is critical for prostate cancer
Authors: Jin Yang , Wang Ling , Qu Su , Sheng Xia , Kristian Alexandr , Mælandsmo Gunhild M , Pällmann Nora , Yuca Erkan , Tekedereli Ibrahim , Gorgulu Kivanc , Alpay Neslihan , Sood Anil , Lopez‐Berestein Gabriel , Fazli Ladan , Rennie Paul , Risberg Bjørn , Wæhre Håkon , Danielsen Håvard E , Ozpolat Bulent , Saatcioglu Fahri 
Published: February 2015

AbstractThe six transmembrane protein of prostate 2 (STAMP2) is an androgen‐regulated gene whose mRNA expression is increased in prostate cancer (PCa). Here, we show that STAMP2 protein expression is increased in human PCa compared with benign prostate that is also correlated with tumor grade and treatment response. We also show that STAMP2 significantly increased reactive oxygen species (ROS) in PCa cells through its iron reductase activity which also depleted NADPH levels. Knockdown of STAMP2 expression in PCa cells inhibited proliferation, colony formation, and anchorage‐independent growth, and significantly increased apoptosis. Furthermore, STAMP2 effects were, at least in part, mediated by activating transcription factor 4 (ATF4), whose expression is regulated by ROS. Consistent with in vitro findings, silencing STAMP2 significantly inhibited PCa xenograft growth in mice. Finally, therapeutic silencing of STAMP2 by systemically administered nanoliposomal siRNA profoundly inhibited tumor growth in two established preclinical PCa models in mice. These data suggest that STAMP2 is required for PCa progression and thus may serve as a novel therapeutic target.
Hepatic stellate cell‐expressed endosialin balances fibrogenesis and hepatocyte proliferation during liver damage
Authors: Mogler Carolin , Wieland Matthias , König Courtney , Hu Junhao , Runge Anja , Korn Claudia , Besemfelder Eva , Breitkopf‐Heinlein Katja , Komljenovic Dorde , Dooley Steven , Schirmacher Peter , Longerich Thomas , Augustin Hellmut G 
Published: February 2015

AbstractLiver fibrosis is a reversible wound‐healing response to injury reflecting the critical balance between liver repair and scar formation. Chronic damage leads to progressive substitution of liver parenchyma by scar tissue and ultimately results in liver cirrhosis. Stromal cells (hepatic stellate cells [HSC] and endothelial cells) have been proposed to control the balance between liver fibrosis and regeneration. Here, we show that endosialin, a C‐type lectin, expressed in the liver exclusively by HSC and portal fibroblasts, is upregulated in liver fibrosis in mouse and man. Chronic chemically induced liver damage resulted in reduced fibrosis and enhanced hepatocyte proliferation in endosialin‐deficient (ENKO) mice. Correspondingly, acute‐liver‐damage‐induced hepatocyte proliferation (partial hepatectomy) was increased in ENKO mice. A candidate‐based screen of known regulators of hepatocyte proliferation identified insulin‐like growth factor 2 (IGF2) as selectively endosialin‐dependent hepatocyte mitogen. Collectively, the study establishes a critical role of HSC in the reciprocal regulation of fibrogenesis vs. hepatocyte proliferation and identifies endosialin as a therapeutic target in non‐neoplastic settings.
Generating Customized Transgene Landing Sites and Multi-Transgene Arrays in Drosophila Using phiC31 Integrase
Authors: Knapp Jon-Michael , Chung Phuong , Simpson Julie H 
Published: February 2015

Abstract
Transgenesis in numerous eukaryotes has been facilitated by the use of site-specific integrases to stably insert transgenes at predefined genomic positions (landing sites). However, the utility of integrase-mediated transgenesis in any system is constrained by the limited number and variable expression properties of available landing sites. By exploiting the nonstandard recombination activity exhibited by a phiC31 integrase mutant, we developed a rapid and inexpensive method for isolating landing sites that exhibit desired expression properties. Additionally, we devised a simple technique for constructing arrays of transgenes at a single landing site, thereby extending the utility of previously characterized landing sites. Using the fruit fly Drosophila melanogaster, we demonstrate the feasibility of these approaches by isolating new landing sites optimized to express transgenes in the nervous system and by building fluorescent reporter arrays at several landing sites. Because these strategies require the activity of only a single exogenous protein, we anticipate that they will be portable to species such as nonmodel organisms, in which genetic manipulation is more challenging, expediting the development of genetic resources in these systems.
Novel Tools for Genetic Manipulation of Follicle Stem Cells in the Drosophila Ovary Reveal an Integrin-Dependent Transition from Quiescence to Proliferation
Authors: Hartman Tiffiney R , Ventresca Erin M , Hopkins Anthony , Zinshteyn Daniel , Singh Tanu , O’Brien Jenny A , Neubert Benjamin C , Hartman Matthew G , Schofield Heather K , Stavrides Kevin P , Talbot Danielle E , Riggs Devon J , Pritchard Caroline , O’Reilly Alana M 
Published: February 2015

Abstract
In many tissues, the presence of stem cells is inferred by the capacity of the tissue to maintain homeostasis and undergo repair after injury. Isolation of self-renewing cells with the ability to generate the full array of cells within a given tissue strongly supports this idea, but the identification and genetic manipulation of individual stem cells within their niche remain a challenge. Here we present novel methods for marking and genetically altering epithelial follicle stem cells (FSCs) within the Drosophila ovary. Using these new tools, we define a sequential multistep process that comprises transitioning of FSCs from quiescence to proliferation. We further demonstrate that integrins are cell-autonomously required within FSCs to provide directional signals that are necessary at each step of this process. These methods may be used to define precise roles for specific genes in the sequential events that occur during FSC division after a period of quiescence.
A Role for the Serine/Arginine-Rich (SR) Protein B52/SRSF6 in Cell Growth and Myc Expression in Drosophila
Authors: Fernando Céline , Audibert Agnès , Simon Françoise , Tazi Jamal , Juge François 
Published: February 2015

Abstract
Serine-/arginine-rich (SR) proteins are RNA-binding proteins that are primarily involved in alternative splicing. Expression of some SR proteins is frequently upregulated in tumors, and previous reports have demonstrated that these proteins can directly participate in cell transformation. Identifying factors that can rescue the effects of SR overexpression in vivo is, therefore, of potential therapeutic interest. Here, we analyzed phenotypes induced by overexpression of the SR protein B52 during Drosophila development and identified several proteins that can rescue these phenotypes. Using the mechanosensory bristle lineage as a developmental model, we show that B52 expression level influences cell growth, but not differentiation, in this lineage. In particular, B52 overexpression increases cell growth, upregulates myc transcription, and gives rise to flies lacking thoracic bristles. Using a genetic screen, we identified several suppressors of the phenotypes induced by overexpression of B52 in vivo in two different organs. We show that upregulation of brain tumor (brat), a tumor suppressor and post-transcriptional repressor of myc, and downregulation of lilliputian (lilli), a subunit of the superelongation complex involved in transcription elongation, efficiently rescue the phenotypes induced by B52 overexpression. Our results demonstrate a role of this SR protein in cell growth and identify candidate proteins that may overcome the effects of SR protein overexpression in mammals.
Histiotus magellanicus (Chiroptera: Vespertilionidae) is not restricted to Subantarctic forests: first record for the Coastal Maulino Forest in central Chile
Authors: Rodríguez-San Pedro Annia , Barquez Rubén M. , Simonetti Javier A. 
Published: February 2015

The southern Big-Eared Brown Bat, Histiotus magellanicus, is a poorly known vespertilionid occurring only in southern Chile and Argentina, where it appears to be restricted to Valdivian Temperate Forests and Magellanic Subpolar Forests. Here we report the first record of H. magellanicus in the Maulino Temperate Forest of central Chile, a Mediterranean-climate ecosystem, suggesting that the species might not be exclusive to subantarctic forests, as previously thought. This constitutes the northernmost continental record for the species, extending its range ca. 400 km the previous known northern locality
CONCENTRACIÓN MAGNÉTICA APLICADA A RELAVE DE COBRE
Authors: Campusano Luis Valderrama , Zazzali Bruno , Santander Mario Enrique , Carmona Manuel 
Published: February 2015

Este trabajo tiene como objetivo desarrollar un circuito de concentración magnética de baja intensidad para tratar relave producido en el tratamiento de minerales de cobre. Para este estudio fueron recolectadas muestras de 27 calicatas construidas y distribuidas a lo largo de la corona del tranque de relaves de la Planta Manuel Antonio Matta de la Empresa Nacional de Minería. El análisis mineralógico indicó la presencia de cuarzo, ortoclasa, magnetita, actinolita, biotita y clinocloro ferroso, albita cálcica, calcita, hematita, pirita, pirrotina y calcopirita. El análisis químico mostró un contenido de hierro total de 18,5% y de hierro magnético de 15,2%. Pruebas realizadas en un Tubo Davis indican que se puede obtener un concentrado con ley de 66,4% de hierro, 3,9% SiO2, 0,70% Al2O3, 0,69% de MgO, 0,60% de CaO y 0,178 de azufre, siendo la recuperación de hierro del 79,2%. Se realizaron pruebas de concentración, a escala de laboratorio, en un separador magnético WLIMS. El concentrado rougher del separador magnético fue remolido hasta una granulometría cuyo P80 es de 50 mm, siguiendo luego pruebas de limpieza, que entregaron un producto final con una ley de hierro de 63,1 %, hierro magnético 61,83 %, azufre 0,23%, SiO2 7%, Al2O3 1,16%, CaO 0,52% y MgO 0,52%.
Editorial
Authors: Leite José Yvan Pereira 
Published: February 2015

EDITORIALEm 2004 a HOLOS opta pela alteração do seu caminho e altera-o para aqueles onde seus artigos são publicados exclusivamente de forma eletrônica e disponibilizados nas nuvens – web. Esta estratégia objetivou ampliar a visibilidade do periódico e, consequentemente, dos artigos publicados. Este processo foi realizado utilizando plataforma de software livre, reduzindo custos e resultando na elevação de downloads e citações de artigos, entre outros.Neste percurso, o número de volumes publicados por ano avançou de forma sistemática, a saber, em 2004 foram publicados dois volumes, entre 2005 a 2008 foram três volumes, em 2009 foram 4 volumes, entre 2010 a 2011 foram quatro volumes e de 2012 aos dias atuais foram seis volumes.Assim, nestes dez anos do periódico online, em ambiente de pleno êxito de relações acadêmicas, foram publicados 46 volumes e 789 artigos. Agora, com caminhos abertos, o foco é consolidar a internacionalização do periódico. Assim, nas edições futuras teremos no mínimo 10% dos artigos em língua inglesa e o periódico estará cada vez mais integrado às ações políticas da Associação Brasileira de Editores Científicos (ABEC).No volume seis de 2014 são apresentados 23 artigos que representam as áreas assim distribuídas: dois na área de empreendedorismo e inovação, três na área de tecnologia mineral e de petróleo, dois na área de tecnologia ambiental, três na área econômica, seis em educação; um de divulgação científica, um de filosofia, um de pedagogia e três de ensino. A diversidade dos artigos publicados reforçam o caráter interdisciplinar e sua internacionalização. Neste contexto de crescimento acadêmico é por demais renovamos os agradecimentos aos autores, pela confiança neste periódico, aos avaliadores ad hoc pelo relevante trabalho e aos membros do conselho editorial pela manutenção dos caminhos editoriais nesta bela trajetória.O momento é de consolidar ações. Neste contexto da globalização é imperioso observar as esquinas, atentar para o convívio, conviver com os passos lentos da sabedoria e vislumbrar ambientes que qualifiquem uma educação de qualidade, fomentar iniciativas de guarda de memória e do desenvolvimento científico e tecnológico.O cenário é convidativo à reflexão. Assim, convido-os a acessarem aqueles artigos que os interessam e interagirem com os autores. Enfim, difundi-los para além deste periódico.Boa leitura! Natal, 13 de fevereiro de 2015Prof. José Yvan Pereira Leite