bluuebunny/crossref_metadata_2025_split huggingface dataset:
Some like it hot: adaptation to the urban heat island in common dandelion
Authors: Woudstra Yannick , Kraaiveld Ron , Jorritsma Alger , Vijverberg Kitty , Ivanovic Slavica , Erkens Roy , Huber Heidrun , Gravendeel Barbara , Verhoeven Koen J F 
Published: August 2024

Abstract
The Urban Heat Island Effect (UHIE) is a globally consistent pressure on biological species living in cities. Adaptation to the UHIE may be necessary for urban wild flora to persist in cities, but experimental evidence is scarce. Here, we report evidence of adaptive evolution in a perennial plant species in response to the UHIE. We collected seeds from common dandelion (Taraxacum officinale) individuals along an urban–rural gradient in the city of Amsterdam (The Netherlands). In common-environment greenhouse experiments, we assessed the effect of elevated temperatures on plant growth and the effect of vernalization treatments on flowering phenology. We found that urban plants accumulate more biomass at higher temperatures and require shorter vernalization periods, corresponding to milder winters, to induce flowering compared to rural plants. Differentiation was also observed between different intra-urban subhabitats, with park plants displaying a higher vernalization requirement than street plants. Our results show genetic differentiation between urban and rural dandelions in temperature-dependent growth and phenology, consistent with adaptive divergence in response to the UHIE. Adaptation to the UHIE may be a potential explanation for the persistence of dandelions in urban environments.
Beyond the flames: understanding the impact of loss without body recovery or identification in fire-related mass disaster contexts
Authors: Barreiro M Beatriz , Morgado Miguel , Ferreira Maria Teresa 
Published: August 2024

Abstract
The World Trade Center attack (2001), the Black Saturday (2009), the Malaysia Airlines Flight 17 (2014), the wildfires of June and October in Portugal (2017), the California wildfires (2018)—these are a few examples of mass disaster events, specifically related to fire and high temperatures, which resulted in multiple casualties and several people unidentified and/or missing. Besides the physical destruction, the lost lives are one of the main consequences of these contexts. Dealing with the loss of a family member may not be an easy process, however, it is easier to accept when there is a body to bury. Conversely, not knowing the whereabouts of a person who might have been involved in a disaster makes mourning and returning to daily life harder. The family of the missing/unidentified lives in doubt and in a state termed as ‘ambiguous loss’. Forensic anthropologists face several challenges and obstacles when detecting and analysing burnt skeletal remains, which are increased in mass disaster contexts. However, the suffering experienced by the families of the victims of these contexts is far superior. Therefore, it is of utmost importance to employ all efforts to achieve the identity of these victims to allow the families to mourn, as well as to reassure society that no death is insignificant. Thus, the importance of identifying the victims of scenarios involving fire/heat is addressed in this paper through a brief description of some mass disaster events, emphasizing the challenges faced in identifying burnt remains and the psychological consequences families endure resulting from it.
α-Mannosylated HLA-II glycopeptide antigens dominate the immunopeptidome of immortalised cells and tumour tissues
Authors: Goodson Hayley , Kawahara Rebeca , Fehring Joshua , Purcell Anthony W , Croft Nathan P , Thaysen-Andersen Morten 
Published: August 2024

Abstract
Immunopeptides are cell surface-located protein fragments that aid our immune system to recognise and respond to pathogenic insult and malignant transformation. In this two-part communication, we firstly summarise and reflect on our recent discovery documenting that MHC-II-bound immunopeptides from immortalised cell lines prevalently carry N-glycans that differ from the cellular glycoproteome (Goodson, Front Immunol, 2023). These findings are important as immunopeptide glycosylation remains poorly understood in immunosurveillance. The study also opened up new technical and biological questions that we address in the second part of this communication. Our study highlighted that the performance of the search engines used to detect glycosylated immunopeptides from LC–MS/MS data remains untested and, importantly, that little biochemical in vivo evidence is available to document the nature of glycopeptide antigens in tumour tissues. To this end, we compared the N-glycosylated MHC-II-bound immunopeptides that were reported from tumour tissues of 14 meningioma patients in the MSFragger-HLA-Glyco database (Bedran, Nat Commun, 2023) to those we identified with the commercial Byonic software. Encouragingly, the search engines produced similar outputs supporting that N-glycosylated MHC-II-bound immunopeptides are prevalent in meningioma tumour tissues. Consistent also with in vitro findings, the tissue-derived MHC-II-bound immunopeptides were found to predominantly carry hyper-processed (paucimannosidic- and chitobiose core-type) and hypo-processed (oligomannosidic-type) N-glycans that varied in prevalence and distribution between patients. Taken together, evidence is emerging suggesting that α-mannosidic glycoepitopes abundantly decorate MHC-II-bound immunopeptides presented in both immortalised cells and tumour tissues warranting further research into their functional roles in immunosurveillance.
The sunflower jacalin Helja: biological and structural insights of its antifungal activity against Candida albicans
Authors: Del Río Marianela V , Radicioni Melisa B , Cutine Anabela M , Mariño Karina V , Mora-Montes Héctor M , Cagnoni Alejandro J , Regente Mariana C 
Published: August 2024

Abstract
The limited availability of efficient treatments for Candida infections and the increased emergence of antifungal-resistant strains stimulates the search for new antifungal agents. We have previously isolated a sunflower mannose-binding lectin (Helja) with antifungal activity against Candida albicans, capable of binding mannose-bearing oligosaccharides exposed on the cell surface. This work aimed to investigate the biological and biophysical basis of Helja’s binding to C. albicans cell wall mannans and its influence on the fungicidal activity of the lectin. We evaluated the interaction of Helja with the cell wall mannans extracted from the isogenic parental strain (WT) and a glycosylation-defective C. albicans with altered cell wall phosphomannosylation (mnn4∆ null mutants) and investigated its antifungal effect. Helja exhibited stronger antifungal activity on the mutant strain, showing greater inhibition of fungal growth, loss of cell viability, morphological alteration, and formation of clusters with agglutinated cells. This differential biological activity of Helja was correlated with the biophysical parameters determined by solid phase assays and isothermal titration calorimetry, which demonstrated that the lectin established stronger interactions with the cell wall mannans of the mnn4∆ null mutant than with the WT strain. In conclusion, our results provide new evidence on the nature of the Helja molecular interactions with cell wall components, i.e. phosphomannan, and its impact on the antifungal activity. This study highlights the relevance of plant lectins in the design of effective antifungal therapies.
The role of coronary artery reimplantation for anomalous right coronary artery originating from the opposite sinus of Valsalva: preliminary outcomes and insights from a Latin American country
Authors: Maldonado-Cañón Kevin , Motta Andrés Felipe , Prada Silvia Alejandra , Maldonado-Escalante Javier 
Published: August 2024

Abstract
Despite promising results, reimplantation appears to have fallen into oblivion among the multiple possible approaches for repairing anomalous coronary arteries. We describe the outcomes of 12 patients with an anomalous right coronary artery originating from the opposite sinus of Valsalva with an interarterial course who were surgically treated with this technique between 2018 and 2023 in 2 institutions in Bogota, Colombia. We provide preliminary evidence of the value reimplantation as a more than suitable technique, particularly in resource-constrained settings. It offers high rates of control of symptoms and functional class recovery while assessing all potential high-risk features, with a low risk of complications, even in middle-aged patients. We also advocate using noninvasive anatomical descriptions and patient symptoms over inducible ischaemia tests in decision making.
A Countrywide Survey of hrp2/3 Deletions and kelch13 Mutations Co-occurrence in Ethiopia
Authors: Kamaliddin Claire , Burke-Gaffney Jack , Ashraf Shoaib , Castañeda-Mogollón Daniel , Adamu Aderaw , Mekonen Tefa Bacha , Wijesinghe Ayesha , Pussegoda Enaara , Feleke Sindew Mekasha , Pillai Dylan R 
Published: August 2024

Abstract
Malaria elimination relies on detection of Plasmodium falciparum histidine-rich proteins 2/3 (HRP2/3) through rapid diagnostic tests (RDTs) and treatment with artemisinin combination therapies (ACTs). Data from the Horn of Africa suggest increasing hrp2/3 gene deletions and ACT partial resistance kelch13 (k13) mutations. To assess this, 233 samples collected during a national survey from 7 regions of Ethiopia were studied for hrp2/3 deletions with droplet digital polymerase chain reaction (ddPCR) and k13 mutations with DNA sequencing. Approximately 22% of the study population harbored complete hrp2/3 deletions by ddPCR. Thirty-two of 44 of k13 single-nucleotide polymorphisms identified were R622I associated with ACT partial resistance. Both hrp2/3 deletions and k13 mutations associated with ACT partial resistance appear to be co-occurring, especially in Northwest Ethiopia. Ongoing national surveillance relying on accurate laboratory methods are required to elaborate the genetic diversity of P. falciparum.
The challenges and opportunities of continuous data quality improvement for healthcare administration data
Authors: Zhang Yili , Callaghan-Koru Jennifer A , Koru Güneş 
Published: August 2024

Abstract

Background
Various data quality issues have prevented healthcare administration data from being fully utilized when dealing with problems ranging from COVID-19 contact tracing to controlling healthcare costs.

Objectives
(i) Describe the currently adopted approaches and practices for understanding and improving the quality of healthcare administration data. (ii) Explore the challenges and opportunities to achieve continuous quality improvement for such data.

Materials and Methods
We used a qualitative approach to obtain rich contextual data through semi-structured interviews conducted at a state health agency regarding Medicaid claims and reimbursement data. We interviewed all data stewards knowledgeable about the data quality issues experienced at the agency. The qualitative data were analyzed using the Framework method.

Results
Sixteen themes emerged from our analysis, collected under 4 categories: (i) Defect characteristics: Data defects showed variability, frequently remained obscure, and led to negative outcomes. Detecting and resolving them was often difficult, and the work required often exceeded the organizational boundaries. (ii) Current process and people issues: The agency adopted primarily ad-hoc, manual approaches to resolving data quality problems leading to work frustration. (iii) Challenges: Communication and lack of knowledge about legacy software systems and the data maintained in them constituted challenges, followed by different standards used by various organizations and vendors, and data verification difficulties. (iv) Opportunities: Training, tool support, and standardization of data definitions emerged as immediate opportunities to improve data quality.

Conclusions
Our results can be useful to similar agencies on their journey toward becoming learning health organizations leveraging data assets effectively and efficiently.
Effect of a behavioral nudge on adoption of an electronic health record-agnostic pulmonary embolism risk prediction tool: a pilot cluster nonrandomized controlled trial
Authors: Richardson Safiya , Dauber-Decker Katherine L , Solomon Jeffrey , Seelamneni Pradeep , Khan Sundas , Barnaby Douglas P , Chelico John , Qiu Michael , Liu Yan , Sanghani Shreya , Izard Stephanie M , Chiuzan Codruta , Mann Devin , Pekmezaris Renee , McGinn Thomas , Diefenbach Michael A 
Published: August 2024

Abstract

Objective
Our objective was to determine the feasibility and preliminary efficacy of a behavioral nudge on adoption of a clinical decision support (CDS) tool.

Materials and Methods
We conducted a pilot cluster nonrandomized controlled trial in 2 Emergency Departments (EDs) at a large academic healthcare system in the New York metropolitan area. We tested 2 versions of a CDS tool for pulmonary embolism (PE) risk assessment developed on a web-based electronic health record-agnostic platform. One version included behavioral nudges incorporated into the user interface.

Results
A total of 1527 patient encounters were included in the trial. The CDS tool adoption rate was 31.67%. Adoption was significantly higher for the tool that included behavioral nudges (39.11% vs 20.66%; P &lt; .001).

Discussion
We demonstrated feasibility and preliminary efficacy of a PE risk prediction CDS tool developed using insights from behavioral science. The tool is well-positioned to be tested in a large randomized clinical trial.

Trial Registration
Clinicaltrials.gov (NCT05203185)
Land investment regulation and allocative efficiency: evidence from the Chinese manufacturing sector
Authors: Zhao Aidong , Ploegmakers Huub , Rouwendal Jan , Ma Xianlei 
Published: August 2024

Abstract
This article analyzes the role of production factor land in the production process. Using a novel dataset of 20,205 newly established firms in the Chinese manufacturing sector from 2007 to 2014, our production function estimates suggest the existence of a sizable gap (averaging 50 USD/m2) between the marginal productivity of land and its user cost. Basic economic theory suggests a possible relationship with China’s minimum investment intensity (MII) regulation. An analysis using changes in MII limits over time and the resulting discontinuities at county borders confirms that larger gaps are significantly associated with increases in MII limits. Thus, the results of this study suggest that MII regulation leads to allocative inefficiency of land.
The MADS-domain transcription factor DAL10 is a direct target of putative DAL1-mediated age pathway in conifers
Authors: Zhang Hui , Wang Peiyi , Song Yitong , Zhao Huanhuan , Zuo Quan , Chen Xi , Han Fangxu , Liu Hongmei , Nie Yumeng , Liu Meiqin , Guo Meina , Niu Shihui 
Published: August 2024

Abstract
The optimal timing of the transition from vegetative growth to reproductive growth is critical for plant reproductive success, and the underlying regulatory mechanisms have been well studied in angiosperm model species, but relatively little in gymnosperms. DAL1, a MADS domain transcription factor (TF) that shows a conserved age-related expression profile in conifers, may be an age timer. However, how DAL1 mediates the onset of reproductive growth remains poorly understood. Here, we showed that PtDAL1 directly regulates PtDAL10 transcription by binding to its promoter region in vitro. Both in vitro and in Nicotiana benthamiana PtDAL1 forms ternary complexes with PtDAL10 and PtMADS11, two potential candidate regulators of the vegetative to reproductive transition in Chinese pine (Pinus tabuliformis). In new shoots PtDAL10 was progressively induced with age and was also expressed in male and female cones. Overexpression of PtDAL10 rescued the flowering of ft-10 and soc1-1-2 mutants in Arabidopsis. We provide insights into the molecular components associated with PtDAL1, which integrates the vegetative to reproductive phase transition into age-mediated progressive development of the whole plant in conifers.
An Investigation of Mucosal Leishmaniasis in the Military Health System
Authors: Pierre James , Davies Julian , Shaikh Saira , Hickey Patrick 
Published: August 2024

ABSTRACT
Leishmaniasis is a protozoal infection with an increased risk of transmission to those serving in the U.S. Military due to theaters of operation in endemic regions. There has, in recent decades, been robust experience with old-world leishmaniasis in the Military Health System (MHS); however, new-world leishmaniasis, which may result in mucosal leishmaniasis, has been less studied. A total of 88 patients from 2012 to 2022 with diagnosis codes for “mucocutaneous leishmaniasis” or “leishmaniasis, unspecified” were identified in the Military Data Repository and reviewed. Within this cohort, upon medical records review, there were 2 cases of leishmaniasis that met inclusion criteria. Case 1 was a 28-year-old active duty male with recent travel to Belize who presented with a mucosal lip lesion that was biopsied and had inconclusive species confirmation but was thought to be either L. braziliensis or L. mexicana. The second case involved a 30-year-old active duty male with a history of travel to French Guiana who had a cutaneous lesion on his left hand that was identified as L. guyanensis, a causative species for mucosal leishmaniasis. Neither had evidence of any further mucosal involvement on otolaryngologic evaluation, and both subsequently received systemic therapy with a good clinical response. Although only 2 cases were identified over an 11-year period, this disease remains an important medical consideration when conducting military operations within Central and South America, as both cases had recent military-specific travel to areas endemic for leishmaniasis.
The impact of stellar metallicity on rotation and activity evolution in the Kepler field using gyro-kinematic ages
Authors: See Victor , Lu Yuxi (Lucy) , Amard Louis , Roquette Julia 
Published: August 2024

ABSTRACT
In recent years, there has been a push to understand how chemical composition affects the magnetic activity levels of main sequence low-mass stars. Results indicate that more metal-rich stars are more magnetically active for a given stellar mass and rotation period. This metallicity dependence has implications for how the rotation periods and activity levels of low-mass stars evolve over their lifetimes. Numerical modelling suggests that at late ages more metal-rich stars should be rotating more slowly and be more magnetically active. In this work, we study the rotation and activity evolution of low-mass stars using a sample of Kepler field stars. We use the gyro-kinematic age dating technique to estimate ages for our sample and use the photometric activity index as our proxy for magnetic activity. We find clear evidence that, at late ages, more metal-rich stars have spun down to slower rotation in agreement with the theoretical modelling. However, further investigation is required to definitively determine whether the magnetic activity evolution occurs in a metallicity dependent way.
Relative alignments between magnetic fields, velocity gradients, and dust emission gradients in NGC 1333
Authors: Chen Michael Chun-Yuan , Fissel Laura M , Sadavoy Sarah I , Rosolowsky Erik , Doi Yasuo , Arzoumanian Doris , Bastien Pierre , Coudé Simon , Di Francesco James , Friesen Rachel , Furuya Ray S , Hwang Jihye , Inutsuka Shu-ichiro , Johnstone Doug , Karoly Janik , Kwon Jungmi , Kwon Woojin , Le Gouellec Valentin J M , Liu Hong-Li , Mairs Steve , Onaka Takashi , Pattle Kate , Rawlings Mark G , Tahani Mehrnoosh , Tamura Motohide , Wang Jia-Wei ,   the BISTRO Team
Published: August 2024

ABSTRACT
Magnetic fields play an important role in shaping and regulating star formation in molecular clouds. Here, we present one of the first studies examining the relative orientations between magnetic (B) fields and the dust emission, gas column density, and velocity centroid gradients on the 0.02 pc (core) scales, using the BISTRO and VLA+GBT observations of the NGC 1333 star-forming clump. We quantified these relative orientations using the Project Rayleigh Statistic (PRS) and found preferential global parallel alignment between the B field and dust emission gradients, consistent with large-scale studies with Planck. No preferential global alignments, however, are found between the B field and velocity gradients. Local PRS calculated for subregions defined by either dust emission or velocity coherence further revealed that the B field does not preferentially align with dust emission gradients in most emission-defined subregions, except in the warmest ones. The velocity-coherent structures, on the other hand, also showed no preferred B field alignments with velocity gradients, except for one potentially bubble-compressed region. Interestingly, the velocity gradient magnitude in NGC 1333 ubiquitously features prominent ripple-like structures that are indicative of magnetohydrodynamic (MHD) waves. Finally, we found B field alignments with the emission gradients to correlate with dust temperature and anticorrelate with column density, velocity dispersion, and velocity gradient magnitude. The latter two anticorrelations suggest that alignments between gas structures and B fields can be perturbed by physical processes that elevate velocity dispersion and velocity gradients, such as infall, accretions, and MHD waves.
Resolution analysis of magnetically arrested disc simulations
Authors: Salas L D S , Musoke G , Chatterjee K , Markoff S B , Porth O , Liska M T P , Ripperda B 
Published: August 2024

ABSTRACT
Polarization measurements by the Event Horizon Telescope from M87* and Sgr A* suggest that there is a dynamically strong, ordered magnetic field, typical of what is expected of a magnetically arrested accretion disc (MAD). In such discs, the strong poloidal magnetic field can suppress the accretion flow and cause episodic flux eruptions. Recent work shows that general relativistic magnetohydrodynamic (GRMHD) MAD simulations feature dynamics of turbulence and mixing instabilities that are becoming resolved at higher resolutions. We perform a convergence study of MAD states exceeding the status quo by an order of magnitude in resolution. We use existing 3D simulations performed with the H-AMR code, up to a resolution of 5376 × 2304 × 2304 in a logarithmic spherical-polar grid. We find consistent time-averaged disc properties across all resolutions. However, higher resolutions reveal signs of inward angular momentum transport attributed to turbulent convection, particularly evident when mixing instabilities occur at the surfaces of flux tubes during flux eruptions. Additionally, we see wave-like features in the jet sheath, which become more prominent at higher resolutions, that may induce mixing between the jet and disc. At higher resolutions, we observe the sheath to be thinner, resulting in increased temperature, reduced magnetization, and greater variability. Those differences could affect the dissipation of energy, which would eventually result in distinct observable radiative emission from high-resolution simulations. With higher resolutions, we can delve into crucial questions about horizon-scale physics and its impact on the dynamics and emission properties of larger-scale jets.