bluuebunny/crossref_metadata_2025_split huggingface dataset:
Radiographic assessment of CBCT-guided autotransplantation of a premolar to the maxillary incisor region: A 2‐year prospective split‐mouth study
Authors: Alzoubi Tamara , EzEldeen Mostafa , Wyatt Jan , Cadenas Maria , Gorp Gertrude Van , Vasconcelos Karla Faria , Jacobs Reinhilde 
Published: August 2024

Abstract

Background: To investigate the 2-year radiographic outcome of cone-beam computed tomography (CBCT)-guided tooth autotransplantation (TAT) of a premolar to the maxillary incisor region in children.
Methods: Eighteen paediatric patients with a missing maxillary incisor were recruited for CBCT-guided premolar transplantation to the maxillary central incisor region (TAT group), neighbouring a healthy contralateral central incisor (control group). Radiological parameters, assessed at 2-year follow-up, included periodontal ligament space (PLS), the outline of lamina dura, the vertical distance between the cementoenamel junction and crestal bone (CEJ-CB), pulp obliteration, radiographic root area (RRA) and length (RRL) and crown-root ratio (CRR).
Results: Positive root growth in 89% of the TAT cases, with an average increase in RRA by 27% compared to the control. Pulp obliteration was observed in 83% of the TAT cases (partial: 55.6%; complete: 27.8%). The average crown-to-root ratio was 0.72 and 1.01 for the control and TAT group, respectively. No significant differences existed between TAT and control concerning PLS (p=0.09), lamina dura (p=0.12), and CEJ-CB distance mesially (p=0.17) or distally (p=0.28).
Conclusions: CBCT-guided TAT might act as a viable treatment option for rehabilitating missing maxillary (centrally) incisor teeth in paediatric patients when osseointegration may not be the preferred option. (Clinical trial center and ethical board University Hospitals, KU Leuven: S55287; ClinicalTrials.gov Identifier: NCT02464202)
Climate adaptation information needs of limited resource farmers in California.
Authors: Taku-Forchu Namah , Koundinya Vikram , Parker Lauren E. , Ikendi Samuel , Pinzón Natalia J. , Thaoxaochay Lilian , Roche Leslie M. , Ostoja Steven , Vong Moua , Dahlquist-Willard Ruth M. , Cooper Mark H. , Pathak Tapan B. 
Published: August 2024

Abstract

California’s agricultural production is facing ever-growing challenges due to climate change. We conducted a needs assessment of farmers in California in 2023, to better understand their climate change challenges, experiences, and resource and information needs. This study employed a convergent parallel mixed-methods design with a questionnaire created and distributed using Qualtrics. Out of the 341 usable survey responses post-data cleaning, 75 selected were limited-resource farmers, socially disadvantaged farmers, and first generation farmers. Two focus groups were conducted in the Central Valley of California involving 19 smallholder Hmong farmers to ascertain the climate-related impacts experienced by these farmers, delve into the strategies to tackle climate change risks, and identify climate change information and resources needed to mitigate challenges. The survey and focus group show similar findings, indicating that farmers are very concerned about several issues, including increased uncertainty in irrigation water availability, increased crop damage due to extreme heat, and worsening drought severity on their farms. Survey and focus group results also revealed farmers are changing their irrigation practices to mitigate climate-related risks, particularly extreme heat and severe drought on their farms. Focus group discussions emphasized adaptations like using shading and adjusting planting seasons to protect crops, while survey results highlighted the adaptation strategies aimed at soil health improvement, including building organic matter, mulching, and adopting reduced or no-till approaches. While already implementing climate adaptation strategies to mitigate risks, these farmers also expressed needs for more education on specific adaptation practices, including weather prediction tools, methods to enhance soil health, and efficient irrigation practices. Additionally, they sought guidance about applying for government funding opportunities, access to insurance, and climate adaptive strategies. Finally, Hmong farmers broadly indicated they preferred in-person workshops featuring climate-related information presented through videos and hands-on activities, which could effectively enhance their ability to adapt to climate change challenges.
Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
Authors: Xia Bin , Li Ziwei , Liu Xiaowei , Yang Yujia , Chen Shengyan , Chen Bin , Li Hongyao , Han Jinxiu , Zhou Yunwei , He Miao 
Published: August 2024

Abstract

Background
Cultivated chrysanthemums are susceptible to abiotic stress, and the intricate polyploidy complicates the discovery of resistance genes. Chrysanthemum indicum is a native diploid species with strong resistance, which makes it an important resource for investigating stress resistance genes and improving genetic traits in ornamental chrysanthemum plants.
Results
In this study, we cloned the CiHY5 gene and generated both overexpressing CiHY5 (OE-CiHY5) and suppressing CiHY5 (RNAi-CiHY5) transgenic chrysanthemum lines. After salt stress treatment, compared with the WT plants, the OE-CiHY5 plants exhibited a lower Malondialdehyde content and less leaf electrolyte leakage and significantly greater antioxidant enzyme activity. In contrast, the physiological parameters of the RNAi-CiHY5 plants exhibited opposite trends. Moreover, the Na+/K+ ratio in both the leaves and roots of the OE-CiHY5 plants significantly decreased in contrast with that in the leaves and roots of the WT and RNAi-CiHY5 plants. The qRT‒PCR results showed that the expression levels of downstream stress response genes, such as CiRAB18, CiERF1, CiABF2, CiABF4, and CiDREB1D, were significantly greater in the OE-CiHY5 plants than in the WT plants. Additionally, a yeast one-hybrid assay revealed that CiHY5 could directly bind to the promoter of CiABF4 and activate CiABF4 expression. Transient overexpression of CiABF4 in C. indicum leaf discs also improved salt stress tolerance.
Conclusions
Overall, we concluded that overexpressing CiHY5 enhanced but RNAi-CiHY5 reduced salt tolerance in C. indicum, acting as a pivotal candidate stress resistance gene that participates in the salt stress response at least partially in an ABA-dependent manner. The above findings demonstrated the molecular mechanisms underlying the CiHY5-mediated salt stress response and laid the foundation for the molecular breeding of chrysanthemum plants to improve resistance.
Integrated pest management practices and pesticide residue in okra among farmers in Kwara State
Authors: Imoloame Emmanuel Oyamedan , Yusuf Olayinka Jelili , Abdulrauf Lukman Bola , Aliyu Taiye Hussein 
Published: August 2024

Abstract

Integrated Pest Management (IPM) is a sustainable approach to minimize the negative impacts of pests on crops while reducing the reliance on synthetic pesticides. This study was conducted in Kwara State Nigeria in 2023, to investigate the implementation of IPM practices and the occurrence of pesticide residues in okra among the farmers in Kwara State, Nigeria. A sample of 245 okra farmers were selected across 4 agricultural zones of Kwara State using a multistage sampling technique. An interview schedule was used to collect information from the farmers. Also, okra samples were collected from the farmers from each community of the Local Government Areas sampled. Data collected from the farmers were analyzed using descriptive and inferential statistics including frequency count, percentage, mean, standard deviation and Pearson Product Moment Correlation (PPMC). The Okra samples collected were taken to the laboratory for pesticide residue extraction using quick, easy, cheap effective rugged and safe (QuEChERS) technique and analysis was done using Gas Chromatography (GS)-Gas Mass spectrometer (MS). Results showed that most okra producers in Kwara State were smallholder farmers who, although practicing IPM, have limited knowledge of the technology. Some pesticide residues were detected in okra, two of which were present in quantities above the maximum residue limit, while others had quantities below the maximum residue limit. Therefore, This study recommends that the government improve extension services in the State by providing adequate funds and training extension agents who will provide comprehensive training to farmers on the types and application of IPM as an eco-friendly technology for preventing pesticide residue in okra.
Prognostic value of response to first-line hydroxyurea according to IPSET stratification in essential thrombocythemia
Authors: Alvarez-Larran Alberto , Santaliestra Marta , Garrote Marta , Noya María Soledad , Pérez-Encinas Manuel , Senín Alicia , Pérez-López Raúl , Ferrer-Marin Francisca , Carreño-Tarragona Gonzalo , Caballero-Navarro Gonzalo , Magro Elena , Velez Patricia , Cortés-Vazquez Miguel Ángel , Moretó Ana , figueras Anna Angona , Pastor-Galán Irene , Guerra José María , Garcia-Hernandez Carmen , Mata María Isabel , Stuckey Ruth , Casares Maria Teresa Gómez , Fox Laura , Cuevas Beatriz , García-Gutiérrez Valentín , Triguero Ana , Arellano-Rodrigo Eduardo , Hernandez-Boluda Juan Carlos 
Published: August 2024

Abstract

Hydroxyurea (HU) constitutes the first-line treatment in most patients with essential thrombocythemia (ET), but criteria for changing therapy are not clearly established. The prognostic value of complete hematological response (CHR) and resistance/intolerance to HU was assessed in 1080 patients from the Spanish Registry of ET, classified according to revised IPSET-Thrombosis stratification (Very low- n = 61, Low- n = 83, Intermediate- n = 261, and High-risk n = 675). With a median therapy duration of 5 years, CHR was registered in 720 (67%) patients (1-year probability 51%) and resistance/intolerance in 219 (20%) patients (5-years probability 13%). After correction by other risk factors, High-risk patients achieving CHR showed a reduced risk of arterial thrombosis (HR: 0.35, 95%CI: 0.2–0.6, p = 0.001) and a trend towards lower risk of venous thrombosis (HR: 0.45, 95%CI: 0.2–1.02, p = 0.06) whereas no association was observed for intermediate- or low-risk patients. In comparison with non-responders, intermediate- and high-risk patients achieving CHR had longer survival and lower myelofibrosis incidence. Development of resistance/intolerance to HU was associated with a higher probability of myelofibrosis but no effect on survival or thrombotic risk was demonstrated. In conclusion, CHR with HU is associated with better outcomes and can be an early indicator for selecting candidates to second line therapy.
rAAV-ENO1mAb-5 inhibits cell proliferation and migration by suppressing glycolysis in cervical cancer
Authors: Yang xiaojuan , Gou yuanfeng , Li Zhenhao , Shuai Ruzhen , Li Fei , Huo Xiaqin , Li Na , Pei Yaping , Han Xue , Ran peng ning , Liu huiling 
Published: August 2024

Abstract

α-enolase (ENO1), as a plasminogen receptor and a key enzyme of glycolysis, is highly expressed in cervical cancer, Inhibition of ENO1 activity can further inhibit the migration and invasion of tumor cells by inhibiting glycolsis, and inhibiting tumor growth.In this study, adeno-associated virus (rAAV-ENO1mAb-5) with the variable region gene of recombinant ENO1 monoclonal antibody was prepared to mediate the targeted entry of the variable region gene of recombinant ENO1 monoclonal antibody into the cytoplasm to observe its growth inhibition effect on cervical cancer cells. The results showed that the variable region gene of rAAV-ENO1mAb-5, which was highly effective and targeted to load ENO1, entered the cytoplasm and inhibited the proliferation and migration of cervical cancer cells by antagonizing ENO1 enzyme activity, significantly reducing lactate and pyruvate content (P < 0.01). In conclusion, rAAV-ENO1mAb-5 can specifically block ENO1 expression on cell membrane and inhibit ENO1 glycolysis activity in tumor cells, which has therapeutic effect on cervical cancer cells.
Optimizing reality-capture for construction monitoring: a study on employing semi-autonomous robots for frequent as-design and as-built comparisons on construction sites.
Authors: Elashry Khaled , Rod Anders , Eltarabishy Sherif , Tsigkari Martha , Davis Adam 
Published: August 2024

Abstract

This paper investigates the application of semi-autonomous robots in automating Construction Progress Monitoring (CPM) and further in the operational phase of the building lifecycle, specifically aimed at enhancing the comparison of as-designed and as-built data in the construction industry. The study illustrates the robot's significant role in the automation of the process of the accusation and alignment of point cloud data, thereby promising considerable time and effort savings over traditional methods. It showcases studies in 3 different locations representing construction, retrofitting and occupied spaces and discusses gains and challenges faced. These advancements help in the creation of more accurate digital twins for both construction and operation.
Assessment of Awareness and Level of Preparedness for Humanitarian Disasters in Nigeria
Authors: Amoo Olufemi Samuel , Awoderu Oluwatoyin Bamidele , Bamidele Moses , Afocha Ebelechukwu Eugenia , Adedeji Abimbola Modepe , Ajayi Morakinyo Bamikole , Bamidele Tajudeen Akanji , Oparaugo Chinedum Taahie , Fesobi Toun Wuraola , Fowora Muinah Adenike , David Nkiru , Mebaghandun Amonetsone Victoria , Umoh Edikan Usiwoma , Musa Adesola Zaidat , Kehinde Akinsinde , Ajayi Abraham , Smith Stella Ifeanyi 
Published: August 2024

Abstract

Background
Disaster preparedness is critical for households, businesses, and communities. Over the years, Nigerians have had to rely on sheer luck and providence to save themselves from disasters with little or no help coming from rescue agencies which on many occasions have been found to lack capacity and wherewithal to intervene in such difficult moments. This research was focused on determining the level of awareness, preparedness and response to humanitarian disasters concerning flood in Nigeria. This is a descriptive cross-sectional study in which relevant data was collected from community dwellers (household heads) selected by a multistage sampling technique and data analysed using SPSS v23.
Results
The mean age of respondents was 39 years with an average family size of seven. Flooding, fire incidence and rioting were the highest recorded disasters known and experienced by the participants and the humanitarian disasters experienced in the four states are statistically significant (p < 0.05). The participant’s awareness of humanitarian disasters emerged from diverse sources with the highest being personal experience (25.5%), television (21.3%) radio jingles (17.9%) and the least information obtained through telephone calls (0.2%). Flooding was the most encountered and significant humanitarian disaster among all participants. Blocked drainages as a cause of the flood were reported amongst 24.9% of respondents while 21.4%, 18.6% and 8.9% attributed the cause of the flood to heavy rainfall, the opening of dams and the lack of drainages, respectively. The effects of flooding on the participants ranged from loss of lives and properties to an adverse impact on income. Also, the study explored how participants would prevent floods. 33.5% responses indicated clearing drainages, 19.3% stated public enlightenment, 13.5% suggested non-dumping of refuse in the drainage and 6.8% affirmed that floods could be prevented if structures are not built on drainage channels. Lastly, concerning what they had to do in case of flooding; 33.2% suggested climbing to a higher area and shouting for help, 25.7% recommended calling emergency numbers, 9.4% proposed swimming across the flood and 7.8% responded with staying in the flood and holding on tightly to something.
Conclusion
To systematically reduce the increased impact of disaster especially flooding among community dwellers, there is need for increased awareness, re-orientation and continued education in response to humanitarian disaster.
Impact of Seasonal Climate and Atmospheric Dynamics on Volcanic Plume Dynamics: A Case Study of Eruptions in the North Polar Region
Authors: Paguem Igor Collins Djouda , Nyam Françoise Martine Enyegue A , Nbendjo Blaise Nana 
Published: August 2024

Abstract

The dynamics of volcanic eruptive columns are significantly influenced by parameters that characterize atmospheric state. While previous studies have predominantly focused on this aspect, this research examines how seasonal variations and insolation that characterize atmosphere dynamics impact dynamics of volcanic eruptive columns. A data analysis method was used. The study analyzed the diurnal cycle of eruptions and investigated the effects of seasonal variability on phreatomagmatic eruptions from the polar atmosphere. The findings provide insights into crucial parameters for modeling plume dynamics. Specifically, the study reveals that (i) atmosphere stability affects plume dynamics, exhibiting greater resistance to plume ascent in winter and reduced resistance in summer, (ii) increased sunny hours enhance plume ascent, and (iii) wind speed has a more pronounced effect on plume dynamics in summer and autumn, diminishing in winter and spring. These results highlight the necessity of incorporating seasonal climate variations into plume dynamics models and underscore the importance of considering eruption timing in relation to atmospheric conditions. A regional dependency of the eruptive column dynamics is noticed and constitute an important result.
Antimicrobial resistance transmission in the environmental settings through Traditional and UV-Enabled Advanced Wastewater Treatment Plants: A metagenomic insight
Authors: Talat Absar , Bashir Yasir , Khalil Nadeem , Brown Connor L , Gupta Dinesh , Khan Asad Ullah 
Published: August 2024

Abstract

Background
Municipal wastewater treatment plants (WWTPs) are pivotal reservoirs for antibiotic-resistance genes (ARGs) and antibiotic-resistant bacteria (ARB). Selective pressures from antibiotic residues, co-selection by heavy metals, and conducive environments sustain ARGs, fostering the emergence of ARB. While advancements in WWTP technology have enhanced the removal of inorganic and organic pollutants, assessing ARG and ARB content in treated water remains a gap. This metagenomic study meticulously examines the filtration efficiency of two distinct WWTPs-conventional (WWTPC) and advanced (WWTPA), operating on the same influent characteristics and located at Aligarh, India.
Results
The dominance of Proteobacteria or Pseudomonadota, characterized the samples from both WWTPs and carried most ARGs. Acinetobacter johnsonii, a prevailing species, exhibited a diminishing trend with wastewater treatment, yet its persistence and association with antibiotic resistance underscore its adaptive resilience. The total ARG count was reduced in effluents, from 58 ARGs, representing 14 distinct classes of antibiotics in the influent to 46 and 21 in the effluents of WWTPC and WWTPA respectively. However, an overall surge in abundance, particularly influenced by genes such as qacL, blaOXA−900, and rsmA was observed. Numerous clinically significant ARGs, including those against aminoglycosides (AAC(6')-Ib9, APH(3'')-Ib, APH(6)-Id), macrolides (EreD, mphE, mphF, mphG, mphN, msrE), lincosamide (lnuG), sulfonamides (sul1, sul2), and beta-lactamases (blaNDM−1), persisted across both conventional and advanced treatment processes. The prevalence of mobile genetic elements and virulence factors in the effluents possess a high risk for ARG dissemination.
Conclusions
Advanced technologies are essential for effective ARG and ARB removal. A multidisciplinary approach focused on investigating the intricate association between ARGs, microbiome dynamics, MGEs, and VFs is required to identify robust indicators for filtration efficacy, contributing to optimized WWTP operations and combating ARG proliferation across sectors.
A Novel Discovery of CXCL5 in Prognosis Prediction and Targeted Therapy of Glioblastomas
Authors: Li Hui , Lu Han , Xi Jianxin , Du Zhishan , Wu Bo , Ren Jiaxin , Yang Wenzhuo , Zhong Sheng 
Published: August 2024

Abstract

Glioblastoma (GBM) patients face a grim prognosis, with many treatments failing to achieve significant improvements. Recent research has focused on the immunosuppressive environment within GBM tumors. One particular protein, C-X-C chemokine ligand 5 (CXCL5), is highly expressed in various cancers and is known to affect the immune environment, tumor invasion, metastasis, and overall prognosis. In our study, we investigated the role of CXCL5 in the immunosuppressive environment of GBM. We aimed to develop a CXCL5-associated immune prognostic signature (IPS) to predict patient outcomes and identify potential treatments targeting the CXCL5/CXCR2 axis. Initially, we performed enzyme-linked immunosorbent assays (ELISA) on 80 high-grade glioma samples to measure CXCL5 levels. We also analyzed RNA-seq data from 169 GBM samples obtained from the TCGA dataset, dividing them into high (CXCL5_H) and low (CXCL5_L) CXCL5 expression groups. Our analysis revealed that the CXCL5_H group had higher expression of immune-related genes but a poorer prognosis compared to the CXCL5_L group. Using the least absolute shrinkage and selection operator (LASSO) Cox analysis, we constructed a CXCL5-associated IPS, which we confirmed as an independent prognostic factor for GBM through univariate and multivariate Cox analyses. We developed a nomogram based on the three-gene IPS to predict overall survival in GBM patients. Moreover, our study identified the CXCL5/CXCR2 axis as a promising target for GBM treatment. We employed computational techniques to screen for potential inhibitors of this axis and validated their effectiveness in vitro. In conclusion, our study provides a new prognostic model and suggests targeted therapeutic options for GBM by elucidating the role of CXCL5 in the tumor's immunosuppressive environment. This work may pave the way for improved patient outcomes and more effective treatments for this challenging cancer.