bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of phylogeny and taxonomy into a comprehensive tree of life
Authors: Hinchliff Cody E. , Smith Stephen A. , Allman James F. , Burleigh J. Gordon , Chaudhary Ruchi , Coghill Lyndon M. , Crandall Keith A. , Deng Jiabin , Drew Bryan T. , Gazis Romina , Gude Karl , Hibbett David S. , Katz Laura A. , Laughinghouse H. Dail , McTavish Emily Jane , Midford Peter E. , Owen Christopher L. , Ree Richard H. , Rees Jonathan A. , Soltis Douglas E. , Williams Tiffani , Cranston Karen A. 
Published: September 2015

SignificanceScientists have used gene sequences and morphological data to construct tens of thousands of evolutionary trees that describe the evolutionary history of animals, plants, and microbes. This study is the first, to our knowledge, to apply an efficient and automated process for assembling published trees into a complete tree of life. This tree and the underlying data are available to browse and download from the Internet, facilitating subsequent analyses that require evolutionary trees. The tree can be easily updated with newly published data. Our analysis of coverage not only reveals gaps in sampling and naming biodiversity but also further demonstrates that most published phylogenies are not available in digital formats that can be summarized into a tree of life.
Light-enhanced bioaccumulation of molybdenum by nitrogen-deprived recombinant anoxygenic photosynthetic bacterium Rhodopseudomonas palustris
Authors: Naito Taki , Sachuronggui  , Ueki Masayoshi , Maeda Isamu 
Published: September 2015

Abstract
As molybdenum (Mo) is an indispensable metal for plant nitrogen metabolisms, accumulation of dissolved Mo into bacterial cells may connect to the development of bacterial fertilizers that promote plant growth. In order to enhance Mo bioaccumulation, nitrogen removal and light illumination were examined in anoxygenic photosynthetic bacteria (APB) because APB possess Mo nitrogenase whose synthesis is strictly regulated by ammonium ion concentration. In addition, an APB, Rhodopseudomonas palustris, transformed with a gene encoding Mo-responsive transcriptional regulator ModE was constructed. Mo content was most markedly enhanced by the removal of ammonium ion from medium and light illumination while their effects on other metal contents were limited. Increases in contents of trace metals including Mo by the genetic modification were observed. Thus, these results demonstrated an effective way to enrich Mo in the bacterial cells by the culture conditions and genetic modification.
Structure and evolutionary history of a large family of NLR proteins in the zebrafish
Authors: Howe Kerstin , Schiffer Philipp H. , Zielinski Julia , Wiehe Thomas , Laird Gavin K. , Marioni John C. , Soylemez Onuralp , Kondrashov Fyodor , Leptin Maria 
Published: September 2015

ABSTRACTAnimals and plants have evolved a range of mechanisms for recognizing noxious substances and organisms. A particular challenge, most successfully met by the adaptive immune system in vertebrates, is the specific recognition of potential pathogens, which themselves evolve to escape recognition. A variety of genomic and evolutionary mechanisms shape large families of proteins dedicated to detecting pathogens and create the diversity of binding sites needed for epitope recognition. One family involved in innate immunity are the NACHT-domain-and Leucine-Rich-Repeat-containing (NLR) proteins. Mammals have a small number of NLR proteins, which are involved in first-line immune defense and recognize several conserved molecular patterns. However, there is no evidence that they cover a wider spectrum of differential pathogenic epitopes. In other species, mostly those without adaptive immune systems, NLRs have expanded into very large families. A family of nearly 400 NLR proteins is encoded in the zebrafish genome. They are subdivided into four groups defined by their NACHT and effector domains, with a characteristic overall structure that arose in fishes from a fusion of the NLR domains with a domain used for immune recognition, the B30.2 domain. The majority of the genes are located on one chromosome arm, interspersed with other large multi-gene families, including a new family encoding proteins with multiple tandem arrays of Zinc fingers. This chromosome arm may be a hot spot for evolutionary change in the zebrafish genome. NLR genes not on this chromosome tend to be located near chromosomal ends.Extensive duplication, loss of genes and domains, exon shuffling and gene conversion acting differentially on the NACHT and B30.2 domains have shaped the family. Its four groups, which are conserved across the fishes, are homogenised within each group by gene conversion, while the B30.2 domain is subject to gene conversion across the groups. Evidence of positive selection on diversifying mutations in the B30.2 domain, probably driven by pathogen interactions, indicates that this domain rather than the LRRs acts as a recognition domain. The NLR-B30.2 proteins represent a new family with diversity in the specific recognition module that is present in fishes in spite of the parallel existence of an adaptive immune system.
A cost analysis of a cancer genetic service model in the UK
Authors: Slade Ingrid , Hanson Helen , George Angela , Kohut Kelly , Strydom Ann , Wordsworth Sarah , Rahman Nazneen ,   MCG Programme
Published: September 2015

BackgroundTechnological advances in DNA sequencing have made gene testing fast and more affordable. Evidence of cost-effectiveness of genetic service models is essential for successful translation, but remain sparse in the literature. In particular there is a lack of cost data related to genetic services.MethodsA detailed micro-costing of 28 pathways relating to breast and/or ovarian cancer and gene testing for theBRCA1andBRCA2genes (termed ‘BRCA testing’) was carried out. These data were combined with patient-level data from a Royal Marsden Cancer Genetics Service audit during which BRCA testing was offered to individuals at ≥10% risk of having a mutation.ResultsThe average cost across all pathways was £2,222.68 (range £376.47-£13,531.24). The average pathway cost for a person with cancer was £1897.71 compared to £2,403.22 for a person without cancer. Of the women seen during audit period, 38% were affected with breast and/or ovarian cancer and 62% were unaffected but concerned about their family history.ConclusionThere is considerable variation in the costs of different gene testing pathways. Improved cost-efficiency could be achieved by increasing the proportion of cancer patients tested, because the pathway cost of an unaffected individual in whom testing has already been performed in a relative with cancer is considerably less.AcknowledgementsWe acknowledge NHS funding to the Royal Marsden/ICR NIHR Specialist Biomedical Research Centre for Cancer. SW is supported by funding through the NIHR Oxford Biomedical Research Centre. This work was supported by Wellcome Trust Award 098518/Z/12/Z. For MCG programme seewww.mcgprogramme.com.Conflict of Interest StatementThere are no conflicts of interests for any author of this paper
Understanding consumer loyalty to technology-based self-services with credence qualities
Authors: Schuster Lisa , Proudfoot Judy , Drennan Judy 
Published: September 2015

Purpose– This paper aims to use the Model of Goal-Directed Behavior (MGB) to examine the factors affecting consumers’ continued use of emerging technology-based self-services (TBSSs) with credence qualities. Professional services, which traditionally require specialized knowledge and high levels of interpersonal interaction to produce owing to their credence qualities, are increasingly delivered via self-service technologies. Health services delivered via mobile devices, for example, facilitate self-care without direct involvement from health professionals.Design/methodology/approach– A mental health service delivered via the Internet and mobile phone, myCompass, was selected as the research context. Twenty interviews were conducted with users of myCompass and the data were thematically analyzed.Findings– The findings of the study showcase the unique determinants of consumers’ continued use of TBSSs with credence qualities relative to the more routine services which have been the focus of extant research. The findings further provide support for the utility of the MGB in explaining service continuance, although the importance of distinguishing between extrinsic and intrinsic motivational components of behavioral desire and capturing the impact of social influence beyond subjective norms is also highlighted.Originality/value– This study contributes to recent research examining differences in consumer responses across TBSSs and behavioral loyalty to these services. It also provides empirical evidence for broadening and deepening the MGB within this behavioral domain.
A study on the user evaluation for an RDA-based Korean bibliography retrieval system
Authors: Do Seulki , Oh Sam G. , Lee Sungin 
Published: September 2015

Purpose– The purpose of this paper is to validate the usefulness of resource description and access (RDA) from user perspectives by implementing an RDA-based bibliographic retrieval system, and comparing it against two retrieval systems.Design/methodology/approach– Surveys and interviews were conducted to gather responses from 20 subjects who used the systems. Usability was measured according to the following metrics: search usefulness from search process and results; search efficiency, measured in time and the number of steps involved; general satisfaction for search results and process, and for information need; satisfaction for search functionalities, with five sub-measures (usability of functions of search tool, appropriateness of search results, usability of additional information, usability of associative relations, and appropriateness of search categories); and system convenience in terms of understandability and ease.Findings– The survey results indicate that all but the satisfaction for appropriateness of search categories showed significant differences between the systems. The interviews show that the RDA system received from the subjects a more positive evaluation compared to the counterpart systems, in search usefulness, search efficiency, general search satisfaction, satisfaction for search functionalities.Practical implications– Though a few organizations such as the Library of Congress in the USA have implemented RDA, no such endeavors have been undertaken in the context of Korean bibliography, and especially for the systematic validation of usability of such a system from user perspectives.Originality/value– This is the first published study that validates the usefulness perceived by users of RDA in the context of Korean bibliography.
Towards a better understanding of the “buffering effects” of organizational virtuousness’ perceptions on employee outcomes
Authors: Nikandrou Irene , Tsachouridi Irene 
Published: September 2015

Purpose– The purpose of this paper is to investigate the buffering effects of organizational virtuousness. More specifically, the study investigates employee reactions (job satisfaction, intent to quit and willingness to support the organization) to organizational virtuousness’ perceptions both in conditions without crisis and in conditions with crisis.Design/methodology/approach– The paper adopts the experimental methodology to explore its main hypotheses and research question. The results of a field study are also presented in order to add generalizability to the experimental results. Apost hocqualitative analysis based on focus-group interviews sheds light on the above findings and enables their better understanding.Findings– The results indicated that even during a financial crisis those perceiving higher organizational virtuousness expressed higher job satisfaction, lower intent to quit and higher willingness to support the organization compared to those perceiving lower organizational virtuousness. Organizational virtuousness’ perceptions have also been found to moderate (accentuate) the effects of the financial crisis on job satisfaction and intent to quit. Willingness to support the organization seems to be unaffected by the financial crisis.Practical implications– Managers should be aware of how individuals respond to organizational virtuousness during conditions of financial crisis.Originality/value– The study makes a unique contribution to the literature by being the first to investigate the effects of organizational virtuousness’ perceptions on employee reactions both pre- and during-financial crisis.
User-oriented elderly care: a validation study in two different settings using observational data
Authors: Kazemi Ali , Kajonius Petri J. 
Published: September 2015

Purpose
– User-oriented care, defined as individualized assisting behaviors, is the dominant approach within elderly care today. Yet, there is little known about its conceptual structure. This paper proposes that user-oriented care has a bi-partite structure which may be decomposed into the two dimensions of task and relation.

Design/methodology/approach
– Care workers were “shadowed” (i.e. observed) at their work (n=391 rated interactions). User-oriented care was assessed along ten process quality indicators targeting the acts of caregiving (i.e. task focus, relation focus, involvement, time-use, body language, autonomy, respect, warmth, encouragement, and information) in two elderly care settings, i.e. home care and nursing home. Observations added up to 45 hours.

Findings
– Principal component analyses confirmed the proposed two-factor structure of user-oriented care. Specifically, the user-oriented care indicators loaded on two distinct factors, i.e. task and relation. The underlying structure of user-oriented care revealed to be invariant across the two settings. However, the results revealed interesting structural differences in terms of explained variance and the magnitude of factor loadings in the home care and nursing home settings. Differences also emerged specifically pertaining to the indicators of autonomy and time-use. These findings suggest that user-oriented behavior may to some extent denote different acts of caregiving and what may be called task- and relation-orientation may be loaded with different meanings in these two care settings.

Originality/value
– This is the first study investigating user-oriented behavior in the context of elderly care using a quantitative observational approach. The authors propose that the observed differences between the two care settings are primarily not due to better elderly care work in home care, but due to some inherent differences between these two contexts of care (e.g. better health and living at home).
Critical issues in spatial distribution of public housing estates and their implications on urban renewal in Hong Kong
Authors: Zheng Wei , Shen Geoffrey , Wang Hao , Lombardi Patrizia 
Published: September 2015

Purpose
– Public housing in Hong Kong plays an essential role in accommodation supply to people of low income. Access to social resources and rent levels of nearby private residential housing are two critical issues impacting the well-being of residents living in public housing estates. However, previous research has rarely focused on the spatial distribution of public housing estates through exploring these two critical issues. On the other hand, Hong Kong is currently experiencing an urban renewal process. It would be beneficial to consider these critical issues for spatial allocation of public housing in urban renewal decision making. The purpose of this paper is to investigate these two critical issues in relation to the spatial distribution of public housing estates in Kowloon, Hong Kong.

Design/methodology/approach
– Seven spatial variables were selected to reflect these critical issues. Spatial analysis in Geographic Information System was conducted to process the data required. Multiple logistic regression was employed to analyse the relationships between the spatial location of public housing estates and the seven selected variables.

Findings
– Based on the analysis results, several problems as well as geographical advantages of the current location of public housing estates were discovered, which can be valuable references for decision making by government authorities for public housing development in the future.

Originality/value
– This research is a pilot study on the spatial distribution of public housing estates and the critical influencing factors in Hong Kong, undertaken by applying both spatial analysis and statistical methods. It can help relevant decision makers deal with current problems of public housing location and make informed decisions on where to locate new housing projects in an urban renewal process, which can increase the equal distribution of social resources and improve the well-being of people living in public housing.
Home owners and developers relationships: exploring the cordiality factor
Authors: Rotimi Funmilayo Ebun , Tookey John Edward , Rotimi James Olabode Bamidele 
Published: September 2015

Purpose
– The cordiality of the relationship between home owners and house developers is significant in the house building sector. This plays a key role in meeting needs, expectations and overall satisfaction. Home owners’ satisfactions are very often dependent on their encounters and experiences with the building organisations they deal with. The purpose of this paper is to explore home owners’ satisfaction to the quality of their new homes by investigating the relationships between them and their house developers when they take possession of their properties.

Design/methodology/approach
– Two distinct lines of investigation were conducted, with the first utilising mail questionnaires administered to new home owners. The second involved interviews with house building developers. Both investigations cover five main regions in New Zealand. However, this study reports the mail questionnaire survey. The data analysis is based on 216 new home owners, and the results presented using descriptive and correlation analysis.

Findings
– This research found that the higher the home owner’s satisfaction with the services provided by the developers, the better the relationship that could exist between them. In addition, the longer it takes for developers to rectify defects that are reported by home owners, the lesser the cordiality of their relationship with their developers. It is also evident from this study that home owners’ relationships with their house developers could be sensitive to the extent of defects in their homes. While it is apparent from this research that new home owners satisfaction level is high, some improvement opportunities exist, which the study highlights for continuous performance improvement. This will enable the residential construction sector to establish more aggressive and pro-active measures of monitoring to the satisfaction of home owners.

Practical implications
– This research provides insight into significant factors that could influence the relationship between home owners and their house developers. The study provides information on changes required in the quality culture that could enable house developers do it once and do it right.

Originality/value
– The originality of this research lies in the provision of a wealth of information on cordiality as a key determinant of home owners and house developers’ relationships. This will enable house developers to set realistic performance standards and focus efforts where they are most needed so that home owners derive satisfaction from the quality of service offered by them.
Insurance and Economic Growth Nexus in Nigeria: Asymmetric Non‐Linear Relationship under Heterogeneous Agents
Authors: Olayungbo D.O. 
Published: September 2015

AbstractThis paper examines the asymmetric non‐linear relationship between insurance and economic growth in Nigeria between 1976 and 2010. Prior studies have examined the direction of causality between insurance and economic growth with mixed conclusions in the insurance‐growth literature. Moreover, the majority of these studies assumed symmetric causal relationships by concentrating mainly on testing the supply‐leading and demand‐following hypothesis. This paper, therefore, contributes to the literature by examining the asymmetric causality test between insurance and economic growth in Nigeria. After the cointegration, the asymmetry causality and the asymmetric impulse responses show a robust significant relationship between high gross domestic product (GDP) and low insurance in the long run.
Novel Blood Purification System for Regulating Excessive Immune Reactions in Severe Sepsis and Septic Shock: An Ex Vivo Pilot Study
Authors: Hara Yoshitaka , Shimomura Yasuyo , Nakamura Tomoyuki , Kuriyama Naohide , Yamashita Chizuru , Kato Yu , Miyasho Taku , Sakai Toshikazu , Yamada Shingo , Moriyama Kazuhiro , Nishida Osamu 
Published: September 2015

AbstractPromising results have been reported with blood purification as adjuvant treatment; however, the immunological mechanisms remain unclear. We have been developing a new blood purification system for regulating excessive immune reactions in severe sepsis and septic shock using a granulocyte adsorbing column (Adacolumn [Ada]), and a cytokine‐adsorbing hemofilter (AN69ST hemofilter [AN69]). Fresh porcine blood was circulated for 6 h in five experimental groups including Ada and AN69 to assess the effects of leukocyte adsorption, phagocytic activity and adhesiveness of granulocytes. In the present study, we found that Ada mainly adsorbed granulocytes and monocytes, but not lymphocytes. The phagocytic activity level of granulocytes decreased, and adhesiveness increased, but the number of CD11b‐positive cells markedly decreased in the current system. Elevated cytokine levels (IL‐1β, IL‐8 and IL‐10) at the outlet of Ada were significantly lower than at the outlet of AN69 due to cytokine adsorption. Further studies are needed to better understand cellular interactions.
Characteristics of Patients and Their Ascites Who Underwent Repeated Cell‐Free and Concentrated Ascites Reinfusion Therapy
Authors: Maeda Sayako , Yabuuchi Junko , Nobuta Hiroshi , Makiishi Tetsuya , Hirose Kunihiko 
Published: September 2015

AbstractNovel cell‐free and concentrated ascites reinfusion therapy (KM‐CART) is easy to use, safe and applicable for refractory ascites. We can get the full amount of ascites, filtrate, and concentrate in a short time. KM‐CART can be applied as palliative care for dying patients including patients with massive malignant ascites. Some patients who underwent repeated KM‐CART survived longer than those who did not repeat the therapy. The aim of this study was to identify the type of patients with ascites for whom KM‐CART would be effective and candidates for repeated KM‐CART. In this retrospective cohort observational study, we examined 123 CART processes performed on 58 patients with refractory ascites. Data were collected before and after processing of the ascites. We compared two groups; patients who underwent KM‐CART ≥ 5 times and those who underwent this process ≤ 4 times. Age, disease, benign or malignant status of the disease, the amount of ascites, concentrations of total protein (TP) and albumin (Alb) and their amounts in the original ascites and the filtered and concentrated ascitic fluid and the recovery ratio of TP and Alb were determined. No significant difference was observed between the two groups in age, disease, amount of ascites, and the recovery ratio of TP and Alb. Significant differences were observed in the amounts of TP and Alb in the original ascites and the filtered and concentrated ascitic fluid. Patients who underwent KM‐CART ≥ 5 times had higher Alb levels in the original ascites than those who underwent this therapy ≤ 4 times. Patients with higher Alb concentrations in the original ascites could be candidates for repeated KM‐CART.