bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Transmembrane Domain and GxxxG Motifs within L2 Are Essential for Papillomavirus Infection
Authors: Bronnimann Matthew P. , Chapman Janice A. , Park Chad K. , Campos Samuel K. 
Published: October 2012

ABSTRACT

During cellular invasion, human papillomavirus type 16 (HPV16) must transfer its viral genome (vDNA) across the endosomal membrane prior to its accumulation at nuclear PML bodies for the establishment of infection. After cellular uptake, the capsid likely undergoes pH-dependent disassembly within the endo-/lysosomal compartment, thereby exposing hidden domains in L2 that facilitate membrane penetration of L2/vDNA complexes. In an effort to identify regions of L2 that might physically interact with membranes, we have subjected the L2 sequence to multiple transmembrane (TM) domain prediction algorithms. Here, we describe a conserved TM domain within L2 (residues 45 to 67) and investigate its role in HPV16 infection.
In vitro
, the predicted TM domain adopts an alpha-helical structure in lipid environments and can function as a real TM domain, although not as efficiently as the bona fide TM domain of PDGFR. An L2 double point mutant renders the TM domain nonfunctional and blocks HPV16 infection by preventing endosomal translocation of vDNA. The TM domain contains three highly conserved GxxxG motifs. These motifs can facilitate homotypic and heterotypic interactions between TM helices, activities that may be important for vDNA translocation. Disruption of some of these GxxxG motifs resulted in noninfectious viruses, indicating a critical role in infection. Using a ToxR-based homo-oligomerization assay, we show a propensity for this TM domain to self-associate in a GxxxG-dependent manner. These data suggest an important role for the self-associating L2 TM domain and the conserved GxxxG motifs in the transfer of vDNA across the endo-/lysosomal membrane.
Pathogenesis of Neonatal Herpes Simplex 2 Disease in a Mouse Model Is Dependent on Entry Receptor Expression and Route of Inoculation
Authors: Kopp Sarah J. , Karaba Andrew H. , Cohen Laura K. , Banisadr Ghazal , Miller Richard J. , Muller William J. 
Published: October 2012

ABSTRACT
Herpes simplex virus (HSV) pathogenesis in mice differs based on availability of the principal entry receptors herpesvirus entry mediator (HVEM) and nectin-1 in a manner dependent upon route of inoculation. After intravaginal or intracranial inoculation of adult mice, nectin-1 is a major mediator of neurologic disease, while the absence of either receptor attenuates disease after ocular infection. We tested the importance of receptor availability and route of infection on disease in mouse models of neonatal HSV. We infected 7-day-old mice lacking neither or one principal HSV receptor or both principal HSV receptors with HSV-2 via a peripheral route (intranasal), via a systemic route (intraperitoneal), or by inoculation directly into the central nervous system (intracranial). Mortality, neurologic disease, and visceral dissemination of virus were significantly attenuated in nectin-1 knockout mice compared with HVEM knockout or wild-type mice after intranasal inoculation. Mice lacking both entry receptors (double-knockout mice) showed no evidence of disease after inoculation by any route. Nectin-1 knockout mice had delayed mortality after intraperitoneal inoculation relative to wild-type and HVEM knockout mice, but virus was able to spread to the brain and viscera in all genotypes except double-knockout mice. Unlike in adult mice, HVEM was sufficient to mediate disease in neonatal mice after direct intracranial inoculation, and the absence of HVEM delayed time to mortality relative to that of wild-type mice. Additionally, in wild-type neonatal mice inoculated intracranially, HSV antigen did not primarily colocalize with NeuN-positive neurons. Our results suggest that differences in receptor expression between adults and newborns may partially explain differences in susceptibility to HSV-2.
Japanese Encephalitis Virus Core Protein Inhibits Stress Granule Formation through an Interaction with Caprin-1 and Facilitates Viral Propagation
Authors: Katoh Hiroshi , Okamoto Toru , Fukuhara Takasuke , Kambara Hiroto , Morita Eiji , Mori Yoshio , Kamitani Wataru , Matsuura Yoshiharu 
Published: October 2012

ABSTRACT

Stress granules (SGs) are cytoplasmic foci composed of stalled translation preinitiation complexes induced by environmental stress stimuli, including viral infection. Since viral propagation completely depends on the host translational machinery, many viruses have evolved to circumvent the induction of SGs or co-opt SG components. In this study, we found that expression of Japanese encephalitis virus (JEV) core protein inhibits SG formation. Caprin-1 was identified as a binding partner of the core protein by an affinity capture mass spectrometry analysis. Alanine scanning mutagenesis revealed that Lys
97
and Arg
98
in the α-helix of the JEV core protein play a crucial role in the interaction with Caprin-1. In cells infected with a mutant JEV in which Lys
97
and Arg
98
were replaced with alanines in the core protein, the inhibition of SG formation was abrogated, and viral propagation was impaired. Furthermore, the mutant JEV exhibited attenuated virulence in mice. These results suggest that the JEV core protein circumvents translational shutoff by inhibiting SG formation through an interaction with Caprin-1 and facilitates viral propagation
in vitro
and
in vivo
.
Reassortment between Two Serologically Unrelated Bluetongue Virus Strains Is Flexible and Can Involve any Genome Segment
Authors: Shaw Andrew E. , Ratinier Maxime , Nunes Sandro Filipe , Nomikou Kyriaki , Caporale Marco , Golder Matthew , Allan Kathryn , Hamers Claude , Hudelet Pascal , Zientara Stéphan , Breard Emmanuel , Mertens Peter , Palmarini Massimo 
Published: October 2012

ABSTRACTCoinfection of a cell by two different strains of a segmented virus can give rise to a “reassortant” with phenotypic characteristics that might differ from those of the parental strains. Bluetongue virus (BTV) is a double-stranded RNA (dsRNA) segmented virus and the cause of bluetongue, a major infectious disease of livestock. BTV exists as at least 26 different serotypes (BTV-1 to BTV-26). Prompted by the isolation of a field reassortant between BTV-1 and BTV-8, we systematically characterized the process of BTV reassortment. Using a reverse genetics approach, our study clearly indicates that any BTV-1 or BTV-8 genome segment can be rescued in the heterologous “backbone.” To assess phenotypic variation as a result of reassortment, we examined viral growth kinetics and plaque sizes inin vitroexperiments and virulence in an experimental mouse model of bluetongue disease. The monoreassortants generated had phenotypes that were very similar to those of the parental wild-type strains bothin vitroandin vivo. Using a forward genetics approach in cells coinfected with BTV-1 and BTV-8, we have shown that reassortants between BTV-1 and BTV-8 are generated very readily. After only four passages in cell culture, we could not detect wild-type BTV-1 or BTV-8 in any of 140 isolated viral plaques. In addition, most of the isolated reassortants contained heterologous VP2 and VP5 structural proteins, while only 17% had homologous VP2 and VP5 proteins. Our study has shown that reassortment in BTV is very flexible, and there is no fundamental barrier to the reassortment of any genome segment. Given the propensity of BTV to reassort, it is increasingly important to have an alternative classification system for orbiviruses.
Ferrocement sandwich and hollow core panels for floor construction
Authors: Fahmy Ezzat H. , Shaheen Yousry B.I. , Abou Zeid Mohamed N. , Gaafar Hassan M. 
Published: October 2012

This paper presents the results of a research work to develop ferrocement sandwich and hollow core panels for use as precast one way slab elements. Sandwich panels consisted of two thin ferrocement layers reinforced with steel wire mesh and a core of autoclaved aerated lightweight concrete bricks. The hollow core panels are made of the same ferrocement mortar matrix with three circular holes of 75 mm diameter running along the length of the specimen. A total of 15 sandwich panels and 12 hollow core panels were cast and tested under flexural loadings. The experimental results showed that high ultimate and service loads, crack resistance control, high ductility, and good energy absorption could be achieved by using the proposed panels. A theoretical model based on the ultimate strength method for reinforced concrete was developed to determine the ultimate moment and mode of failure of the panels. The theoretical and experimental results showed good agreement.
Clinical Implication of Adenosine Test at Repeat Atrial Fibrillation Ablation Procedure
Authors: Miyazaki Shinsuke , Kobori Atsushi , Komatsu Yuki , Kuwahara Taishi , Uchiyama Takashi , Kusa Shigeki , Taniguchi Hiroshi , Hachiya Hitoshi , Hirao Kenzo , Isobe Mitsuaki , Takahashi Atsushi , Iesaka Yoshito 
Published: October 2012

Background—
Pulmonary vein reconnection after electrical isolation is commonly observed in the context of atrial fibrillation ablation and is associated with recurrent atrial tachyarrhythmias. Adenosine test was been performed to identify acute dormant conduction immediately after pulmonary vein isolation at index procedure. However, the utility of adenosine test at repeat procedure has not been reported.

Methods and Results—
We report 5 paroxysmal atrial fibrillation cases without any structural heart disease in which dormant thoracic vein conduction was associated with recurrent atrial tachyarrhythmias. All patients had undergone circumferential ipsilateral pulmonary vein isolation at the index procedure. Superior vena cava isolation was performed if superior vena cava–triggered atrial fibrillation was identified. At the index procedure, adenosine test did not provoke venous reconduction. At the repeat procedure, adenosine provoked clinical arrhythmia in 4 out of 5 cases after transient reconnection between culprit thoracic vein and atrium despite absence of reconnection at the start of the procedure. After the elimination of the dormant conduction gaps, all patients were free from recurrent arrhythmia.

Conclusions—
Adenosine provokes dormant thoracic vein conduction associated with the late recurrence of atrial tachyarrhythmias after previous thoracic vein isolation. Thus, adenosine provocation test can specifically help identify and target the cause of recurrent atrial arrhythmia.
Connecting Changing Ocean Circulation with Changing Climate
Authors: Winton Michael , Griffies Stephen M. , Samuels Bonita L. , Sarmiento Jorge L. , Frölicher Thomas L. 
Published: October 2012

AbstractThe influence of changing ocean currents on climate change is evaluated by comparing an earth system model’s response to increased CO2 with and without an ocean circulation response. Inhibiting the ocean circulation response, by specifying a seasonally varying preindustrial climatology of currents, has a much larger influence on the heat storage pattern than on the carbon storage pattern. The heat storage pattern without circulation changes resembles carbon storage (either with or without circulation changes) more than it resembles the heat storage when currents are allowed to respond. This is shown to be due to the larger magnitude of the redistribution transport—the change in transport due to circulation anomalies acting on control climate gradients—for heat than for carbon. The net ocean heat and carbon uptake are slightly reduced when currents are allowed to respond. Hence, ocean circulation changes potentially act to warm the surface climate. However, the impact of the reduced carbon uptake on radiative forcing is estimated to be small while the redistribution heat transport shifts ocean heat uptake from low to high latitudes, increasing its cooling power. Consequently, global surface warming is significantly reduced by circulation changes. Circulation changes also shift the pattern of warming from broad Northern Hemisphere amplification to a more structured pattern with reduced warming at subpolar latitudes in both hemispheres and enhanced warming near the equator.
A Dynamical Initialization Scheme for Real-Time Forecasts of Tropical Cyclones Using the WRF Model*
Authors: Cha Dong-Hyun , Wang Yuqing 
Published: October 2012

Abstract
To improve the initial conditions of tropical cyclone (TC) forecast models, a dynamical initialization (DI) scheme using cycle runs is developed and implemented into a real-time forecast system for northwest Pacific TCs based on the Weather Research and Forecasting (WRF) Model. In this scheme, cycle runs with a 6-h window before the initial forecast time are repeatedly conducted to spin up the axisymmetric component of the TC vortex until the model TC intensity is comparable to the observed. This is followed by a 72-h forecast using the Global Forecast System (GFS) prediction as lateral boundary conditions. In the DI scheme, the spectral nudging technique is employed during each cycle run to reduce bias in the large-scale environmental field, and the relocation method is applied after the last cycle run to reduce the initial position error. To demonstrate the effectiveness of the proposed DI scheme, 69 forecast experiments with and without the DI are conducted for 13 TCs over the northwest Pacific in 2010 and 2011. The DI shows positive effects on both track and intensity forecasts of TCs, although its overall skill depends strongly on the performance of the GFS forecasts. Compared to the forecasts without the DI, on average, forecasts with the DI reduce the position and intensity errors by 10% and 30%, respectively. The results demonstrate that the proposed DI scheme improves the initial TC vortex structure and intensity and provides warm physics spinup, producing initial states consistent with the forecast model, thus achieving improved track and intensity forecasts.
Effect of the adjunct of carbon dioxide during cardiopulmonary bypass on cerebral oxygenation
Authors: Quarti A , Nardone S , Manfrini F , D’Orfeo F , Genova S , Silvano R , Pozzi M 
Published: October 2012

Introduction: Over the last few years, near-infrared spectroscopy (NIRS) has been introduced to study cerebral haemodynamics and oxygenation. This paper points out how the use of an external source of CO2 effects on the absolute value of cerebral NIRS during cardiac surgery on cardiopulmonary bypass. Patients and methods: Between January 2010 and September 2011, 368 patients underwent congenital heart disease correction on cardiopulmonary bypass (CPB). Nineteen patients, with a mean age of 26 days (range 6-120 days), required an external source of CO2 to correct hypocarbia during cardiopulmonary bypass. Different parameters were monitored: NIRS value, oxygen saturation, oxygen partial pressure, CO2 partial pressure, haematocrit, mean arterial pressure and pH. They were analyzed during different phases of the surgical procedure: before, during and after CO2 infusion. Results: There were no deaths during the hospital stay. The NIRS value increased significantly (p<0.05) after the addition of CO2, from a starting value of 52.9 to a final value of 63.4. PaCO2 was found to increase too: from 31.3 mmHg to 40.6 mmHg. On the other hand, both values decreased when the CO2 was removed, to respective final values of 55.8 and 34.4 mmHg. Mean arterial pressure, haematocrit and PaO2 didn’t modify significantly during this period. Conclusions: Nowadays, NIRS is usually used in cardiac surgery to reduce possible risks of neurological damage. The importance of the role of pCO2 in the cerebral vascular resistance and in cerebral blood flow has already been proven. This research demonstrates a relationship between pCO2 and the NIRS value. This paper could introduce an important correcting tool when an inadequate NIRS value occurs although the level of oxygenation, haematocrit and mean arterial pressure are acceptable and the arterial line is accurately positioned.
Factors Associated With the Clinical Outcomes of the Osteochondral Autograft Transfer System in Osteochondral Lesions of the Talus
Authors: Kim Yong Sang , Park Eui Hyun , Kim Yong Chan , Koh Yong Gon , Lee Jin Woo 
Published: October 2012

Background: Identifying factors associated with the clinical outcomes of the osteochondral autograft transfer system would be helpful for treating patients with an osteochondral lesion of the talus. Purpose: To investigate the clinical and second-look arthroscopic results of the osteochondral autograft transfer system and to identify the prognostic factors associated with this procedure. Study Design: Case series; Level of evidence, 4. Methods: The authors retrospectively evaluated 52 ankles that underwent osteochondral autograft transfer for a medial osteochondral lesion of the talus. Second-look arthroscopies were performed at a mean of 13.1 months postoperatively. Clinical outcomes were evaluated according to a visual analog scale (VAS) for pain, American Orthopaedic Foot and Ankle Society (AOFAS) score, and the Tegner activity scale. Statistical analyses were performed to identify various prognostic factors associated with the clinical outcomes. Results: The mean VAS, AOFAS, and Tegner activity scale scores were all significantly improved from 6.9 ± 0.9 to 3.3 ± 1.4 (VAS), from 67.4 ± 4.9 to 82.6 ± 7.8 (AOFAS), and from 3.0 ± 0.8 to 3.9 ± 0.9 (Tegner; P < .05). Regarding overall patient satisfaction with the operation, 49 (95%) patients reported good to excellent results. Prognostic factors including the patient’s age, sex, body mass index, duration of symptoms, defect size and depth, location of osteochondral lesion of the talus, and the existence of a subchondral cyst did not significantly influence clinical outcomes ( P > .05), except for body mass index on the Tegner activity scale score ( P = .021). Significant differences were observed among clinical outcomes for second-look arthroscopy according to the presence of soft tissue impingement and uncovered areas around the graft ( P < .05). The VAS and AOFAS score at the last follow-up were significantly worse when the articular surface of the tibial plafond at the malleolar osteotomy site was uneven ( P = .031 and .012, respectively). Conclusion: This study showed that the articular surface of the tibial plafond at the malleolar osteotomy site, soft tissue impingement, and uncovered areas around the graft were important factors affecting the clinical outcomes, as observed through second-look arthroscopy. Therefore, surgeons should restore the articular surface accurately after the osteotomy, and more caution should be taken to avoid soft tissue impingement and uncovered areas around the graft when performing osteochondral autograft transfer.
Tissue damage in kidney, adrenal glands and diaphragm following extracorporeal shock wave lithotripsy
Authors: Gecit İlhan , Kavak Servet , Oguz Elif Kaval , Pirincci Necip , Günes Mustafa , Kara Mikail , Ceylan Kadir , Kaba Mehmet , Tanık Serhat 
Published: October 2012

This study was designed to investigate whether exposure to short-term extracorporeal shock wave lithotripsy (ESWL) produces histologic changes or induces apoptosis in the kidney, adrenal glands or diaphragm muscle in rats. The effect of shock waves on the kidney of male Wistar rats ( n = 12) was investigated in an experimental setting using a special ESWL device. Animals were killed at 72 h after the last ESWL, and the tissues were stained with an in situ Cell Death Detection Kit, Fluorescein. Microscopic examination was performed by fluorescent microscopy. Apoptotic cell deaths in the renal tissue were not observed in the control group under fluorescent microscopy. In the ESWL group, local apoptotic changes were observed in the kidney in the area where the shock wave was focused. The apoptotic cell deaths observed in the adrenal gland of the control group were similar to those observed in the ESWL groups, and apoptosis was occasionally observed around the capsular structure. Apoptotic cell deaths in the diaphragm muscle were infrequently observed in the control group. Apoptosis in the ESWL group was limited to the mesothelial cells. This study demonstrated that serious kidney, adrenal gland and diaphragm muscles damage occurred following ESWL, which necessitated the removal of the organ in the rat model. It is recognized that the ESWL complications related to the kidney, adrenal gland and diaphragm muscles are rare and may be managed conservatively.
Can the ABILHAND handle manual ability in MS?
Authors: Barrett LE , Cano SJ , Zajicek JP , Hobart JC 
Published: October 2012

Background: Hand dysfunction is common in multiple sclerosis (MS). Recent interest has focused on incorporating patient-reported outcome (PRO) instruments into clinical trials. Nevertheless, examinations are rare in MS of existing manual ability measures. Objectives: The objective of this paper is to evaluate the 23-item ABILHAND, developed for use after stroke, in people with MS, comparing the findings from two psychometric approaches. Methods: We analysed ABILHAND data from 300 people with MS using: 1) traditional psychometric methods (data completeness, scaling assumptions, reliability, internal and external construct validity); and 2) Rasch measurement methods (including targeting, item response category ordering, data fit to the Rasch model, spread of item locations, item scoring bias, item stability, reliability, person response validity). Results: Traditional psychometric methods implied ABILHAND was reliable and valid in this sample. Rasch measurement methods supported this finding. The three-category scoring function worked as intended and item fit to Rasch model expectations was acceptable. The 23 items (location range −3.16 to +2.73 logits) mapped a continuum of manual ability. Reliability was high (Person Separation Index (PSI) = 0.95). Conclusion: Both psychometric evaluations supported ABILHAND as a robust manual ability PRO measure for MS. Rasch measurement methods were more informative and, consistent with its role of detecting anomalies, identified ways of advancing further ABILHAND’s measurement performance to reduce any potential for type II errors in clinical trials.
Left Ventricular Aneurysm in a Four-Year-Old Child
Authors: Negus A. C. M. , Occleshaw C. , Gentles T. L. , Artrip J. H. 
Published: October 2012

We describe spontaneous rupture of a congenital left ventricular (LV) aneurysm with subsequent tamponade and cardiac arrest in a 4-year-old male with staphylococcal septicemia. Emergency resuscitation, thoracotomy, and oversewing were successfully undertaken in the pediatric intensive care unit. There was complete cardiovascular recovery without adverse neurodevelopmental sequelae. This article details the difficulties in determining the etiology of ventricular aneurysms but highlights the importance of attempting to do so, particularly in distinguishing between congenital and acquired forms. Congenital aneurysms are usually a stable pathology; mycotic aneurysms are not and should be managed emergently, as survival after rupture is rare.