bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treating Scars After Burns With Pulsed Electric Fields in the Rat Model
Authors: Mann Din , Bar-Shai Nurit , Levkov Klimentiy , Gabay Batel , Vitkin Edward , Nyska Abraham , Yarmush Martin , Shalom Avshalom , Golberg Alexander 
Published: August 2024

Abstract
Reducing scar size after severe burn injuries is an important and challenging medical, technological, and social problem. We have developed a battery-powered pulsed electric field (PEF) device and surface needle electrode applicator to deliver PEFs to the healing dorsal burn wound in rats. The pulsed electric field was used to treat residual burn wounds caused by metal contact in rats starting 10 days after the injury for 4 months every 11 or 22 days for 4 months using varying time applied voltages at 250-350 V range, 400 mA current, 40 pulses, 70 μs duration each, delivered at pulse repetition frequency 10 Hz at 5 locations inside the wound. We found 40%-45% reduction in the scar size in comparison with untreated controls in both upper and lower dorsal locations on rats’ backs 2 months after the last PEF application. We have not detected significant histopathological differences in the center of the scars besides the thickness of the newly generated epidermis, which was thicker in the PEF-treated group. We showed that minimally invasively applied PEFs through needle electrodes are effective method and device for treating residual burn wounds in the rat model, reducing the size of the resulting scars, without any adverse reaction.
‘China’s Disequilibrium’ in ISDS: an interplay of China’s trade-offs and domestic institutions to investment treaty policy
Authors: Lin Yating 
Published: August 2024

Abstract
This article explores China’s limited engagement in Investor-State Dispute Settlment (ISDS) despite its significant foreign investment inflows, termed “China’s Disequilibrium”. Despite having liberal ISDS provisions in its Bilateral Investment Treaties(BITs), China has faced only nine claims and has never lost a case. Using a political-economic framework, the article examines domestic factors influencing China's stance on ISDS, highlighting its shift from ideological resistance to acceptance. The paper argues that China’s ambivalent mood to ISDS render it to adopt a bifurcated strategy: internationally, it incrementally liberalizes investor-State Arbitration to protect Chinese investors abroad; domestically, it relies on local remedy to protect foreign investors and discourages the use of investment treaty arbitration. This approach contrasts with other jurisdictions and partly explains China’s Disequilibrium in ISDS.
Spontaneous bilothorax without previous surgery or trauma, a case report
Authors: Neetichow Tarashene , Angthong Wirana , Tongyoo Assanee 
Published: August 2024

Abstract
Bilothorax is a rare condition that can lead to severe infection and death. Most cases present with right-sided pleural effusion and the etiology can be biliary obstruction, infection, or iatrogenic complications. The diagnosis of bilothorax is confirmed by the ratio of pleural fluid to serum bilirubin &gt;1. A 33-year-old Asian female presented with progressive dyspnea from right pleural effusion, which was confirmed to be biloma by pleural fluid to serum bilirubin ratio of 15.9. Imaging showed right-sided subdiaphragmatic nodule, which was subsequently biopsied on laparoscopy revealing hemorrhagic endometriotic lesion. However, there was no obvious diaphragmatic defect connecting pleural and peritoneal cavities. Additionally, no biliary leakage was identified by magnetic resonance cholangiopancreatography (MRCP) or endoscopic retrograde cholangiopancreatography (ERCP). The treatment included antibiotics, tube thoracostomy, ERCP with stent, thermal ablation of endometriotic nodules under laparoscopy, and hormonal therapy for endometriosis. Bilothorax is rare case itself but the etiology secondary to endometriosis makes this case particularly unique.
The Book Collection of John Ostewyk, Chaplain of St Magnus the Martyr, London, 1495
Authors: Payne M T W 
Published: August 2024

Abstract
This note offers a transcription, and brief discussion, of the probate inventory of John Ostewyk, a priest in the parish of St Magnus the Martyr in London, who died in 1495. Among his belongings, the appraisers found seventeen named volumes and twenty pamphlets. These are additional to books mentioned in his will. Some of the volumes can be identified with surviving editions.
William Worcestre, Sir John Fastolf and Latin Learning
Authors: Rundle David 
Published: August 2024

Abstract
Sir John Fastolf is known for his association with books in the vernaculars of French and English. It is, though, known that there was also an interest in Latin learning in his circle, and this article provides new evidence concerning that. It introduces a set of manuscripts which passed from the hands of Fastolf’s secretary, William Worcestre, to the foundation of Magdalen College, Oxford. Their passage to Oxford is placed in the context of the battle over Fastolf’s will, with its desire to establish a college at his house of Caister in Norfolk. A college did not necessarily imply a learned function but what Worcestre perceived to be the intentions for that foundation are placed in a wider context of his belief in his late master’s interest in promoting Latin education.
The Military Medicine Leadership Life Cycle: A Model for Longitudinal Leadership Development for Staff Physicians
Authors: Hartzell Joshua D , Servey Jessica , Wilson Ramey , Mount George , Barry Erin S , Durning Steven J 
Published: August 2024

ABSTRACT
Leadership development is a challenge for all health care systems. Military Medicine has unique challenges with increased frequency of physician turnover and more junior leaders taking on positions of leadership earlier in their careers. Military medical corps officers are also challenged with leading in clinical, academic, and operational settings. Effective leadership within the Military Healthcare System requires an intentional and ongoing leadership development process across the careers of military medical corps officers. This article describes the leadership lifecycle of military medical corps officers, highlighting existing leadership development opportunities and providing an example of a leadership lifecycle from junior staff to senior executive for other organizations. The article concludes with specific recommendations that will allow military medicine to continue to strengthen the leadership skills of its officers to meet ever growing challenges.
The ratio of [Eu/α] differentiates accreted/in situ Milky Way stars across metallicities, as indicated by both field stars and globular clusters
Authors: Monty Stephanie , Belokurov Vasily , Sanders Jason L , Hansen Terese T , Sakari Charli M , McKenzie Madeleine , Myeong GyuChul , Davies Elliot Y , Ardern-Arentsen Anke , Massari Davide 
Published: August 2024

ABSTRACT
We combine stellar orbits with the abundances of the heavy, r-process element europium and the light, $\alpha$-element, silicon to separate in situ and accreted populations in the Milky Way (MW) across all metallicities. At high orbital energy, the accretion-dominated halo shows elevated values of [Eu/Si], while at lower energies, where many of the stars were born in situ, the levels of [Eu/Si] are lower. These systematically different levels of [Eu/Si] in the MW and the accreted halo imply that the scatter in [Eu/$\alpha$] within a single galaxy is smaller than previously thought. At the lowest metallicities, we find that both accreted and in situ populations trend down in [Eu/Si], consistent with enrichment via neutron star mergers. Through compiling a large data set of abundances for 54 globular clusters (GCs), we show that differences in [Eu/Si] extend to populations of in situ/accreted GCs. We interpret this consistency as evidence that in r-process elements GCs trace the star formation history of their hosts, motivating their use as sub-Gyr timers of galactic evolution. Furthermore, fitting the trends in [Eu/Si] using a simple galactic chemical evolution model, we find that differences in [Eu/Si] between accreted and in situ MW field stars cannot be explained through star formation efficiency alone. Finally, we show that the use of [Eu/Si] as a chemical tag between GCs and their host galaxies extends beyond the Local Group, to the halo of M31 – potentially offering the opportunity to do Galactic Archaeology in an external galaxy.
BioFlow-Insight: facilitating reuse of Nextflow workflows with structure reconstruction and visualization
Authors: Marchment George , Brancotte Bryan , Schmit Marie , Lemoine Frédéric , Cohen-Boulakia Sarah 
Published: August 2024

Abstract
Bioinformatics workflows are increasingly used for sharing analyses, serving as a cornerstone for enhancing the reproducibility and shareability of bioinformatics analyses. In particular, Nextflow is a commonly used workflow system, permitting the creation of large workflows while offering substantial flexibility. An increasing number of Nextflow workflows are being shared on repositories such as GitHub. However, this tremendous opportunity to reuse existing code remains largely underutilized. In cause, the increasing complexity of workflows constitute a major obstacle to code reuse. Consequently, there is a rising need for tools that can help bioinformaticians extract valuable information from their own and others’ workflows. To facilitate workflow inspection and reuse, we developed BioFlow-Insight to automatically analyze the code of Nextflow workflows and generate useful information, particularly in the form of visual graphs depicting the workflow’s structure and representing its individual analysis steps. BioFlow-Insight is an open-source tool, available as both a command-line interface and a web service. It is accessible at https://pypi.org/project/bioflow-insight/ and https://bioflow-insight.pasteur.cloud/.
Ceftolozane/Tazobactam for the Treatment of Adults With Cystic Fibrosis: Results From a French Prospective Cohort Study
Authors: Burgel Pierre-Régis , Bourge Xavier , Mackosso Carole , Parquin Francois 
Published: August 2024

Abstract

Background
People with cystic fibrosis (pwCF) are particularly susceptible to respiratory infections, including those caused by multidrug-resistant (MDR) pathogens. Ceftolozane/tazobactam (C/T) is an antibacterial agent combination active against MDR gram-negative bacteria that has shown promising results in isolates from pwCF. This subanalysis is the first extensive observation of real-world C/T use in pwCF.

Methods
The multicenter observational CONDUCT study included consecutive patients, some with cystic fibrosis, who received ≥1 dose of C/T at 28 centers throughout France. Patients were treated according to hospital standards and followed up until the end of C/T treatment (EOT).

Results
Among 260 patients who had received ≥1 dose of C/T, 63 were pwCF, including 12 with previous lung transplant. The median age was 34 years and 55.6% of patients were female. Pseudomonas aeruginosa was the most frequently isolated pathogen (n = 40/41 [97.6%]). Most tested P aeruginosa strains (n = 65/73 [91.5%]) and all other isolated strains (Escherichia coli, Citrobacter koseri, Proteus mirabilis, and Serratia marcescens) were susceptible to C/T. Most patients completed the treatment duration, including those with historical β-lactam hypersensitivity. Reasons for stopping treatment were planned EOT and improvement in condition; overall, 88.9% of patients (n = 56/63) experienced improvement in condition. No new safety signals were identified. Mean forced expiratory volume in 1 second improved from 1.33 L to 1.47 L before and after C/T treatment, respectively (n = 52; P = .057).

Conclusions
C/T treatment was well tolerated and effective in pwCF, including those with previous β-lactam hypersensitivity.
Clinical immunity to malaria involves epigenetic reprogramming of innate immune cells
Authors: Nideffer Jason , Ty Maureen , Donato Michele , John Rek , Kajubi Richard , Ji Xuhuai , Nankya Felistas , Musinguzi Kenneth , Press Kathleen Dantzler , Yang Nora , Camanag Kylie , Greenhouse Bryan , Kamya Moses , Feeney Margaret E , Dorsey Grant , Utz Paul J , Pulendran Bali , Khatri Purvesh , Jagannathan Prasanna 
Published: August 2024

Abstract
The regulation of inflammation is a critical aspect of disease tolerance and naturally acquired clinical immunity to malaria. Here, we demonstrate using RNA sequencing and epigenetic landscape profiling by cytometry by time-of-flight, that the regulation of inflammatory pathways during asymptomatic parasitemia occurs downstream of pathogen sensing—at the epigenetic level. The abundance of certain epigenetic markers (methylation of H3K27 and dimethylation of arginine residues) and decreased prevalence of histone variant H3.3 correlated with suppressed cytokine responses among monocytes of Ugandan children. Such an epigenetic signature was observed across diverse immune cell populations and not only characterized active asymptomatic parasitemia but also correlated with future long-term disease tolerance and clinical immunity when observed in uninfected children. Pseudotime analyses revealed a potential trajectory of epigenetic change that correlated with a child's age and recent parasite exposure and paralleled the acquisition of clinical immunity. Thus, our data support a model whereby exposure to Plasmodium falciparum induces epigenetic changes that regulate excessive inflammation and contribute to naturally acquire clinical immunity to malaria.
Robust differential gene expression patterns in the prefrontal cortex of male mice exposed to an occupationally relevant dose of laboratory-generated wildfire smoke
Authors: Schuller Adam , Oakes Jessica , LaRocca Tom , Matz Jacqueline , Eden Matthew , Bellini Chiara , Montrose Luke 
Published: August 2024

Abstract
Wildfires have become common global phenomena concurrent with warmer and drier climates and are now major contributors to ambient air pollution worldwide. Exposure to wildfire smoke has been classically associated with adverse cardiopulmonary health outcomes, especially in vulnerable populations. Recent work has expanded our understanding of wildfire smoke toxicology to include effects on the central nervous system and reproductive function; however, the neurotoxic profile of this toxicant remains ill-explored in an occupational context. Here, we sought to address this by using RNA sequencing to examine transcriptomic signatures in the prefrontal cortex of male mice modeling career wildland firefighter smoke exposure. We report robust changes in gene expression profiles between smoke-exposed samples and filtered air controls, evidenced by 2,862 differentially expressed genes (51.2% increased). We further characterized the functional relevance of these genes highlighting enriched pathways related to synaptic transmission, neuroplasticity, blood–brain barrier integrity, and neurotransmitter metabolism. Additionally, we identified possible contributors to these alterations through protein–protein interaction network mapping, which revealed a central node at ß-catenin and secondary hubs centered around mitochondrial oxidases, the Wnt signaling pathway, and gene expression machinery. The data reported here will serve as the foundation for future experiments aiming to characterize the phenotypic effects and mechanistic underpinnings of occupational wildfire smoke neurotoxicology.
Aryl hydrocarbon receptor-dependent toxicity by retene requires metabolic competence
Authors: Rude Christian I , Wilson Lindsay B , La Du Jane , Lalli Priscila M , Colby Sean M , Schultz Katherine J , Smith Jordan N , Waters Katrina M , Tanguay Robyn L 
Published: August 2024

Abstract
Polycyclic aromatic hydrocarbons (PAHs) are a class of organic compounds frequently detected in the environment with widely varying toxicities. Many PAHs activate the aryl hydrocarbon receptor (AHR), inducing the expression of a battery of genes, including xenobiotic metabolizing enzymes like cytochrome P450s (CYPs); however, not all PAHs act via this mechanism. We screened several parent and substituted PAHs in in vitro AHR activation assays to classify their unique activity. Retene (1-methyl-7-isopropylphenanthrene) displays Ahr2-dependent teratogenicity in zebrafish, but did not activate human AHR or zebrafish Ahr2, suggesting a retene metabolite activates Ahr2 in zebrafish to induce developmental toxicity. To investigate the role of metabolism in retene toxicity, studies were performed to determine the functional role of cyp1a, cyp1b1, and the microbiome in retene toxicity, identify the zebrafish window of susceptibility, and measure retene uptake, loss, and metabolite formation in vivo. Cyp1a-null fish were generated using CRISPR-Cas9. Cyp1a-null fish showed increased sensitivity to retene toxicity, whereas Cyp1b1-null fish were less susceptible, and microbiome elimination had no significant effect. Zebrafish required exposure to retene between 24 and 48 hours post fertilization (hpf) to exhibit toxicity. After static exposure, retene concentrations in zebrafish embryos increased until 24 hpf, peaked between 24 and 36 hpf, and decreased rapidly thereafter. We detected retene metabolites at 36 and 48 hpf, indicating metabolic onset preceding toxicity. This study highlights the value of combining molecular and systems biology approaches with mechanistic and predictive toxicology to interrogate the role of biotransformation in AHR-dependent toxicity.
Wedelolactone inhibits ferroptosis and alleviates hyperoxia-induced acute lung injury via the Nrf2/HO-1 signaling pathway
Authors: Li Kang , Wang Xiao-Qin , Liao Zhen-Liang , Liu Jun-Ya , Feng Bang-Hai , Ren Ying-Cong , Dai Ni-Nan , Yu Kun , Yu Hong , Chen Hua-Jun , Mei Hong , Qin Song 
Published: August 2024

Abstract
Hyperoxia-induced acute lung injury (HALI) is a complication of oxygen therapy. Ferroptosis is a vital factor in HALI. This paper was anticipated to investigate the underlying mechanism of wedelolactone (WED) on ferroptosis in HALI. The current study used hyperoxia to injure two models, one HALI mouse model and one MLE-12 cell injury model. We found that WED treatment attenuated HALI by decreasing the lung injury score and lung wet/dry (W/D) weight ratio and alleviating pathomorphological changes. Then, the inflammatory reaction and apoptosis in HALI mice and hyperoxia-mediated MLE-12 cells were inhibited by WED treatment. Moreover, WED alleviated ferroptosis with less iron accumulation and reversed expression alterations of ferroptosis markers, including MDA, GSH, GPX4, SLC7A11, FTH1, and TFR1 in hyperoxia-induced MLE-12 cells in vitro and in vivo. Nrf2-KO mice and Nrf2 inhibitor (ML385) decreased WED’s ability to protect against apoptosis, inflammatory response, and ferroptosis in hyperoxia-induced MLE-12 cells. Collectively, our data highlighted the alleviatory role of WED in HALI by activating the Nrf2/HO-1 pathway.
Feature-agnostic metabolomics for determining effective subcytotoxic doses of common pesticides in human cells
Authors: Rivera Emilio S , LeBrun Erick S , Breidenbach Joshua D , Solomon Emilia , Sanders Claire K , Harvey Tara , Tseng Chi Yen , Thornhill M Grace , Blackwell Brett R , McBride Ethan M , Luchini Kes A , Alvarez Marc , Williams Robert F , Norris Jeremy L , Mach Phillip M , Glaros Trevor G 
Published: August 2024

Abstract
Although classical molecular biology assays can provide a measure of cellular response to chemical challenges, they rely on a single biological phenomenon to infer a broader measure of cellular metabolic response. These methods do not always afford the necessary sensitivity to answer questions of subcytotoxic effects, nor do they work for all cell types. Likewise, boutique assays such as cardiomyocyte beat rate may indirectly measure cellular metabolic response, but they too, are limited to measuring a specific biological phenomenon and are often limited to a single cell type. For these reasons, toxicological researchers need new approaches to determine metabolic changes across various doses in differing cell types, especially within the low-dose regime. The data collected herein demonstrate that LC-MS/MS-based untargeted metabolomics with a feature-agnostic view of the data, combined with a suite of statistical methods including an adapted environmental threshold analysis, provides a versatile, robust, and holistic approach to directly monitoring the overall cellular metabolomic response to pesticides. When employing this method in investigating two different cell types, human cardiomyocytes and neurons, this approach revealed separate subcytotoxic metabolomic responses at doses of 0.1 and 1 µM of chlorpyrifos and carbaryl. These findings suggest that this agnostic approach to untargeted metabolomics can provide a new tool for determining effective dose by metabolomics of chemical challenges, such as pesticides, in a direct measurement of metabolomic response that is not cell type-specific or observable using traditional assays.
Wind-Mediated Dispersal of Beet Leafhoppers and Pine Pollen in Southern Idaho
Authors: Strausbaugh Carl A. , Wenninger Erik J. , Jackson Laurie K. , Vincill Eric 
Published: August 2024

The management of beet leafhoppers (BLHs) is important for disease control since BLHs can vector important plant pathogens such as curly top viruses and phytoplasmas in southern Idaho. Historical data for southern Idaho suggests that BLHs need approximately 130 growing degree days (GDDs; 12.8°C base) to initiate dispersal and around 382 GDDs until they reach peak dispersal. A recent study in southern Idaho identified large peak dispersal events of BLHs on 19 May 2020 and 2 June 2021 in Elmore County near Mt. Home, ID. Historically, BLHs have been thought to originate from local areas. However, based on GDDs and dispersal numbers under optimal conditions for Mt. Home, the BLHs likely did not originate from local areas. Data for wind and pine pollen dispersal combined with GDDs for areas known to contain BLHs suggest that the BLHs could have originated outside the local area and possibly up to 142 to 515 km away. At least five conditions appear to be necessary for observation of BLH dispersal into southern Idaho: a wind event must occur (35 km/h average hourly wind speed), dispersal temperature threshold (16 to 18°C) must be met, >130 GDDs must be accumulated to initiate dispersal, daily peak temperatures should reach 24°C, and attractive BLH vegetation such as Russian thistle must be present. Combining wind event forecasts with temperature parameters in the future may make it possible to provide targeted timely insecticide sprays for BLH control. [Formula: see text] The author(s) have dedicated the work to the public domain under the Creative Commons CC0 “No Rights Reserved” license by waiving all of his or her rights to the work worldwide under copyright law, including all related and neighboring rights, to the extent allowed by law, 2024.