bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital fabrication of custom tracheostomy appliances: A clinical report
Authors: Frias Vladimir , Wachowiak Lindsay 
Published: July 2024

AbstractThe use of rapid prototyping technology has revolutionized the fabrication of intraoral prostheses. With the advancement of digital technology, its applications have expanded to extraoral prostheses and appliances to replace a variety of head and neck defects. The following clinical report illustrates the use of a new technique that allows the digital replication and recontouring of a stock tracheostomy tube to improve patient fit, comfort, and esthetics.
Comparative plastome and mitogenome analyses indicate that the marine prasinophyte green algae Pycnococcus provasolii and Pseudoscourfieldia marina (Pseudoscourfieldiophyceae class nov., Chlorophyta) represent morphotypes of the same species
Authors: Crépeault Olivier , Otis Christian , Pombert Jean‐François , Turmel Monique , Lemieux Claude 
Published: July 2024

AbstractThe marine prasinophyte green algae Pycnococcus provasolii and Pseudoscourfieldia marina represent the only extant genera and known species of the Pycnococcaceae. However, their taxonomic status needs to be reassessed, owing to the very close relationship inferred from previous sequence comparisons of individual genes. Although Py. provasolii and Ps. marina are morphologically different, their plastid rbcL and nuclear small subunit rRNA genes were observed to be nearly or entirely identical in sequence, thus leading to the hypothesis that they represent distinct growth forms or alternate life‐cycle stages of the same organism. To evaluate this hypothesis, we used organelle genomes as molecular markers. The plastome and mitogenome of Ps. marina UIO 007 were sequenced and compared with those available for two isolates of Py. provasolii (CCMP 1203 and CCAP 190/2). The Ps. marina organelle genomes proved to be almost identical in size and had the same gene content and gene order as their Py. provasolii counterparts. Single nucleotide substitutions and insertions/deletions were localized using genome‐scale sequence alignments. Over 99.70% sequence identities were observed in all pairwise comparisons of plastomes and mitogenomes. Alignments of both organelle genomes revealed that Ps. marina UIO 007 is closer to Py. provasolii CCAP 190/2 than are the two Py. provasolii strains to one another. Therefore, our results are not consistent with the placement of Ps. marina and Py. provasolii strains into distinct genera. We propose a taxonomic revision of the Pycnococcaceae and the erection of a new class of Chlorophyta, the Pseudoscourfieldiophyceae.
Stapled functional end‐to‐end intestinal anastomosis with endovascular gastrointestinal anastomosis staplers in cats and small dogs
Authors: Genoni S. , Cinti F. , Pilot M. , Rossanese M. , McCready D. , Cantatore M. 
Published: July 2024

ObjectivesTo investigate the use of endovascular gastrointestinal stapling devices to perform intestinal functional end‐to‐end stapled anastomosis in small dogs and cats.Materials and MethodsMedical records of dogs (≤10 kg) and cats that underwent intestinal resection and functional end‐to‐end stapled anastomosis with an endovascular gastrointestinal anastomosis (endovascular‐GIA) stapling device at five small animal referral centres between April 2014 and September 2023 were retrospectively reviewed. Data including clinical findings, surgical technique, histopathology and complications were collected. A minimum follow‐up of 10 days was required. Patients with follow‐up of less than 10 days were included if they developed a major complication. Outcome was obtained from assessing the clinical records and contacting the referring veterinarians or owners. Estimated survival was generated according to the Kaplan–Meier method. Differences between survival curves were tested by log‐rank test.ResultsTwenty‐five patients (10 dogs and 15 cats) were included. The median bodyweight was 4.6 kg (range 2.6 to 10 kg). Nine patients were diagnosed with intestinal neoplasia, 16 with non‐neoplastic intestinal disease. The median follow‐up was 126 days (range 18 to 896 days). Five patients developed minor postoperative complications, including three superficial surgical site infections. No major postoperative complications were reported. Eighteen patients were alive at the end of the study, one patient was lost to follow‐up. Kaplan–Meier estimated median survival time was not reached. Survival was significantly longer for patients with non‐neoplastic versus neoplastic intestinal.Clinical SignificanceThe study suggests that the use of endovascular gastrointestinal anastomosis staplers is safe and effective to perform intestinal functional end‐to‐end stapled anastomosis in dogs ≤10 kg and cats.
Physics‐informed neural operator solver and super‐resolution for solid mechanics
Authors: Kaewnuratchadasorn Chawit , Wang Jiaji , Kim Chul‐Woo 
Published: July 2024

AbstractPhysics‐Informed Neural Networks (PINNs) have solved numerous mechanics problems by training to minimize the loss functions of governing partial differential equations (PDEs). Despite successful development of PINNs in various systems, computational efficiency and fidelity prediction have remained profound challenges. To fill such gaps, this study proposed a Physics‐Informed Neural Operator Solver (PINOS) to achieve accurate and fast simulations without any required data set. The training of PINOS adopts a weak form based on the principle of least work for static simulations and a strong form for dynamic systems in solid mechanics. Results from numerical examples indicated that PINOS is capable of approximating solutions notably faster than the benchmarks of PINNs in both static an dynamic systems. The comparisons also showed that PINOS reached a convergence speed of over 20 times faster than finite element software in two‐dimensional and three‐dimensional static problems. Furthermore, this study examined the zero‐shot super‐resolution capability by developing Super‐Resolution PINOS (SR‐PINOS) that was trained on a coarse mesh and validated on fine mesh. The numerical results demonstrate the great performance of the model to obtain accurate solutions with a speed up, suggesting effectiveness in increasing sampling points and scaling a simulation. This study also discusses the differentiation methods of PINOS and SR‐PINOS and suggests potential implementations related to forward applications for promising machine learning methods for structural designs and optimization.
Nomen omen: Euglena gracilis possesses a rhodopsin‐based photoreceptor
Authors: Lorenzetti Giulia , Barsanti Laura , Birindelli Lorenzo , Gualtieri Paolo , Legnaioli Stefano 
Published: July 2024

AbstractThe unicellular microalga Euglena gracilis has always been considered the ideal alga to investigate photoreceptive responses and systems, and it has been the subject of hundreds of articles. Moreover, because of its detectable photoreceptor, it has been given a key role in the evolution of photoreception, from single and simple cells to complex visual system of higher organisms. In this article, we report the Raman spectra recorded in vivo on photoreceptors of E. gracilis and Bos taurus retina. The almost perfect superimposability (correlation coefficient r = 0.955) of these spectra states that the Euglena possesses a photoreceptor with the same structural characteristic of a vertebrate photoreceptor, i.e. a stack of membrane layers embedding rhodopsin‐like proteins. Raman spectra recorded in vivo on photoreceptors of E. gracilis after hydroxylamine treatment further confirm our findings, which should lead to a reconsideration of most of the scientific literature on algae photoreception and eye evolution.
Factors influencing job satisfaction and professional competencies in clinical practice among internationally educated nurses during the migration journey: A mixed‐methods systematic review
Authors: Cubelo Floro , Al Jabri Fatma , Jokiniemi Krista , Turunen Hannele 
Published: July 2024

AbstractBackgroundThe increasing migration of internationally educated nurses (IENs) from developing to developed countries highlights the importance of implementing integration strategies that facilitate their workplace transition, leading to improved job satisfaction and professional competence.AimThe mixed‐methods systematic review aimed to synthesise the current literature on factors influencing job satisfaction and professional competencies among IENs throughout their transition process, including the pre‐migration, migration, and post‐migration periods.MethodsThis study conducted a mixed‐methods systematic review from 2013 to 2023, using the CINAHL, Scopus, and PubMed databases and employing a Population, Interventions, Comparisons, Outcomes (PICO) framework. Quality assessment employed the Mixed Methods Appraisal Tool (MMAT), and data analysis followed a convergent parallel design. Data synthesis was presented narratively, and the literature review adhered to Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) guidelines.ResultsOut of 565 articles, eleven studies (eight qualitative, two quantitative, and one mixed‐method) met the inclusion criteria. Three key themes that influenced job satisfaction and professional competencies among IENs were identified: pre‐migration and pre‐deployment demands, challenges in workplace integration, and post‐migration career challenges and retention strategies.LimitationsThe study primarily focused on IENs recruited from developing countries, potentially limiting the generalisability of the findings to IENs from other regions or countries. Furthermore, the inclusion of nurses from developed countries who also migrated was limited in the available studies, which further restricts the applicability of the research findings.ConclusionsIt is essential to reconsider the pre‐migration language requirements and evaluate the feasibility of completing them during the integration period to enhance the job satisfaction and professional competencies of IENs. Additionally, key factors for improving job satisfaction among IENs include providing personalised support, addressing managerial barriers, facilitating career advancement, efficiently managing workloads, and promoting effective communication within the healthcare team.
Emotional reactivity and past self‐injurious behavior moderate the association between trauma exposure and fearlessness about death
Authors: Stumps Anna D. , Bounoua Nadia , Sheehan Ana E. , Sadeh Naomi 
Published: July 2024

AbstractIntroductionAs suicide remains a global public health concern, recent work has sought to characterize mechanisms underlying the transition from suicidal ideation to action. Acquired capability for suicide, or fearlessness about death, has been identified as one key factor underlying this transition; however, understanding how this capability emerges remains limited. This study sought to extend previous work on the correlates of fearlessness about death by examining its relationship with painful and provocative events and emotional reactivity.MethodsWe tested the extent to which trait emotional reactivity and past self‐injurious behavior moderated the relationship between assaultive trauma exposure and fearlessness about death in a diverse sample of 273 community adults (aged 18–55, M/SD = 32.77/10.78).ResultsA three‐way interaction emerged, such that among individuals with heightened emotional reactivity and a history of self‐injurious behavior (suicide attempt or non‐suicidal self‐injury), assaultive trauma was associated with increased fearlessness about death. In contrast, among adults with low emotional reactivity and a history of self‐injurious behavior, assaultive trauma was associated with reduced fearlessness about death.ConclusionsResults suggest that emotional reactivity may be a key dispositional factor that influences how trauma exposure and self‐injurious behavior impact fearlessness about death.
Worlding and weirding with beaver: A more‐than‐human political ecology of ecosystem engineering
Authors: Lorimer Jamie 
Published: July 2024

AbstractScientists and policy‐makers promote 'Nature‐based Solutions' to the interconnected challenges associated with the Anthropocene. Often these involve the strategic use of ecosystem engineers: animals, plants, and microbes with disproportionate ecological agency capable of regional or even planetary‐scale niche construction. This environmental mode of biopolitics is promoted as biomimicry: restoring, rewilding, or rewetting diverse ecological systems. This paper critically examines the multispecies relations promised by this model through a focus on beaver in Britain over the last 12,000 years. It begins with beaver making Britain hospitable for early settlers and agriculturalists as they returned after the last ice age. It traces the subsequent demise of beaver due to hunting and land use change, and then follows the recent return of beaver as tools for natural flood management and nature recovery. It attends to situations in which these multispecies world‐making projects go awry in the weird ecologies of the Anthropocene. This story of beaver helps situate enthusiasms for proactive ecosystem engineering in deeper time. It highlights the beguiling potential of Nature‐based Solutions while cautioning against tendencies towards anthropocentrism, an apolitical mononaturalism, and an ecomodernist hubris. The paper combines concepts from archaeology, ecology, anthropology, and geography into a new framework for theorising multispecies acts of worlding and weirding.
Development and implementation of a core genome multilocus sequence typing scheme for
Yersinia enterocolitica:
a tool for surveillance and outbreak detection
Authors: Pires Joao , Brandal Lin T. , Naseer Umaer 
Published: July 2024

ABSTRACT

Yersinia enterocolitica
(
Y. enterocolitica
) is the most frequent etiological agent of yersiniosis and has been responsible for several national outbreaks in Norway and elsewhere. A standardized high-resolution method, such as core genome Multilocus Sequence Typing (cgMLST), is needed for pathogen traceability at the national and international levels. In this study, we developed and implemented a cgMLST scheme for
Y. enterocolitica
. We designed a cgMLST scheme in SeqSphere + using high-quality genomes from different
Y. enterocolitica
biotype sublineages. The scheme was validated if more than 95% of targets were found across all tested
Y. enterocolitica
: 563 Norwegian genomes collected between 2012 and 2022 and 327 genomes from public data sets. We applied the scheme to known outbreaks to establish a threshold for identifying major complex types (CTs) based on the number of allelic differences. The final cgMLST scheme included 2,582 genes with a median of 97.9% (interquartile range 97.6%–98.8%) targets found across all tested genomes. Analysis of outbreaks identified all outbreak strains using single linkage clustering at four allelic differences. This threshold identified 311 unique CTs in Norway, of which CT18, CT12, and CT5 were identified as the most frequently associated with outbreaks. The cgMLST scheme showed a very good performance in typing
Y. enterocolitica
using diverse data sources and was able to identify outbreak clusters. We recommend the implementation of this scheme nationally and internationally to facilitate
Y. enterocolitica
surveillance and improve outbreak response in national and cross-border outbreaks.
Initiation of H1-T6SS dueling between
Pseudomonas aeruginosa
Authors: George M. , Narayanan S. , Tejada-Arranz A. , Plack A. , Basler M. 
Published: July 2024

ABSTRACT

The Type VI secretion system (T6SS) is a multicomponent apparatus, present in many Gram-negative bacteria, which can inhibit bacterial prey in various ecological niches.
Pseudomonas aeruginosa
assembles one of its three T6SS (H1-T6SS) to respond to attacks from adjacent competing bacteria. Surprisingly, repeated assemblies of the H1-T6SS, termed dueling, were described in a monoculture in the absence of an attacker strain; however, the underlying mechanism was unknown. Here, we explored the role of H2-T6SS of
P. aeruginosa
in triggering H1-T6SS assembly. We show that H2-T6SS inactivation in
P. aeruginosa
causes a significant reduction in H1-T6SS dueling and that H2-T6SS activity directly triggers retaliation by the H1-T6SS. Intraspecific competition experiments revealed that elimination of H2-T6SS in non-immune prey cells conferred protection from H1-T6SS. Moreover, we show that the H1-T6SS response is triggered independently of the characterized lipase effectors of the H2-T6SS, as well as those of
Acinetobacter baylyi
and
Vibrio cholerae
. Our results suggest that H1-T6SS response to H2-T6SS in
P. aeruginosa
can impact intraspecific competition, particularly when the H1-T6SS effector-immunity pairs differ between strains, and could determine the outcome of multistrain colonization.

IMPORTANCE

The opportunistic pathogen
Pseudomonas aeruginosa
harbors three different Type VI secretion systems (H1, H2, and H3-T6SS), which can translocate toxins that can inhibit bacterial competitors or inflict damage to eukaryotic host cells. Unlike the unregulated T6SS assembly in other Gram-negative bacteria, the H1-T6SS in
P. aeruginosa
is precisely assembled as a response to various cell damaging attacks from neighboring bacterial cells. Surprisingly, it was observed that neighboring
P. aeruginosa
cells repeatedly assemble their H1-T6SS toward each other. Mechanisms triggering this “dueling” behavior between sister cells were unknown. In this report, we used a combination of microscopy, genetic and intraspecific competition experiments to show that H2-T6SS initiates H1-T6SS dueling. Our study highlights the interplay between different T6SS clusters in
P. aeruginosa
, which may influence the outcomes of multistrain competition in various ecological settings such as biofilm formation and colonization of cystic fibrosis lungs.
MBX-7591: a promising drug candidate against drug-resistant fungal infections
Authors: Pereira de Sa Nivea , Del Poeta Maurizio 
Published: July 2024

ABSTRACT

Invasive fungal infections (IFIs) caused by pathogenic fungi pose a significant public health concern, particularly for immunocompromised individuals. Mortality rates for IFIs remain high, and currently available treatment options are limited. Existing antifungal agents often suffer from limited clinical efficacy, poor fungicidal activity within the host, potential toxicity, and increasing ineffectiveness due to emerging resistance, especially against triazole drugs, the current mainstay of antifungal treatment. A recent study has identified MBX-7591, a small molecule with promising antifungal activity against
Aspergillus fumigatus
and other pathogenic fungi, including strains resistant to triazoles (C. Gutierrez-Perez, C. Puerner, J. T. Jones, S. Vellanki, E. M. Vesely, et al., mBio e01166-24, 2024,
https://doi.org/10.1128/mbio.01166-24
). This novel compound appears to inhibit stearoyl-CoA 9-desaturase, a key enzyme involved in fungal fatty acid biosynthesis. By disrupting the conversion of saturated fatty acids to oleic acid, MBX-7591 offers a unique mechanism of action, potentially reducing the risk of resistance development. Here, we now discuss the implications of these groundbreaking findings for overcoming antifungal drug resistance.
Draft genome sequence of
Nitrobacter vulgaris
DSM 10236
T
Authors: Soghomonian Mark , Soghomonian Angela , Escobar Matthew , Thiel Vera , Göker Markus , Ivanova Natalia , Seshadri Rekha , Maitra Kalyani 
Published: July 2024

ABSTRACT

Here, we report the draft genome sequence of
Nitrobacter vulgaris
DSM 10236
T
, a nitrite-oxidizing bacterium isolated from a sewage system in Hamburg, Germany. The genome is 4.3 Mb in size with 4,585 predicted genes, including the full complement of genes necessary for growth on nitrite (
narK, nxrA, nxrB, nxrC,
and
nxrD
).
CLinical EValuation of WEB 17 device in intracranial aneuRysms (CLEVER): 1-year effectiveness results for ruptured and unruptured aneurysms
Authors: Spelle Laurent , Costalat Vincent , Caroff Jildaz , Wodarg Fritz , Fischer Sebastian , Herbreteau Denis , Möhlenbruch Markus A , Januel Anne-Christine , Papagiannaki Chrysanthi , Klisch Joachim , Numminen Jussi , Rautio Riitta , Berlis Ansgar , Mihalea Cristian , Chalumeau Vanessa , Downer Jonathan , Cortese Jonathan , Ikka Leon , Gallas Sophie , Bester Maxim , Liebig Thomas , Velasco Stéphane , Grimaldi Lamiae , Byrne James , Szikora Istvan , Pierot Laurent , Cognard Christophe 
Published: July 2024

BackgroundThe Woven EndoBridge (WEB) device is designed to treat wide-necked bifurcation aneurysms. The WEB 17 is the latest iteration and can be delivered through a 0.017″ microcatheter. The CLEVER study demonstrated that WEB 17 is safe and effective for providing protection against bleeding or rebleeding at 1 month and 1 year.ObjectiveTo evaluate angiographic stability at 1 year.MethodsThe CLEVER study was a prospective multicenter study conducted in 17 European centers, involved 163 subjects, comprising 60 ruptured and 103 unruptured aneurysms. Independent assessment of 1-year follow-up imaging was incorporated into the study design.ResultsAneurysm diameters ranged from 2.0 to 9.2 mm, with 95.7% being broad-based (dome-to-neck ratio <2). Follow-up imaging at 1 year was completed for 146 out of 163 subjects (89.6%) and evaluated by an independent core laboratory. The primary efficacy endpoint of adequate occlusion without re-treatment at 1 year was achieved for 120 (82.2%) of all subjects. At 1 year, the adequate occlusion rate was 86.5% for ruptured aneurysms (73.1% complete occlusion) and 82.4% for unruptured aneurysms (57.1% complete occlusion). The overall re-treatment rate at 1 year was 2.6% (4/152), with 3.1% (3/97) for unruptured aneurysms and 1.8% (1/55) for ruptured aneurysmsConclusionDelivery of the WEB 17 via 0.017 inch catheters represents a significant evolution of the WEB design. The results of CLEVER presented here demonstrate that it maintains the same efficacy as previous generations of WEB.
Pilot epinephrine dose-finding study to counter epidural-related blood pressure reduction
Authors: Nin Olga C , Boezaart Andre , Giordano Christopher , Hughes Steven J , Parvataneni Hari K , Reina Miguel A , Schirmer Abigail , Vasilopoulos Terrie 
Published: July 2024

ObjectiveAn unwanted side effect associated with epidural analgesia is the reduction in blood pressure (BP) due to the sympathetic blockade. This study evaluated the hemodynamic effects of adding different epinephrine concentrations to epidurally injected local anesthetic solution to counteract sympathectomy. We hypothesized that epinephrine could mitigate the decrease in BP possibly caused by the local anesthetic, specifically decreasing the incidence of hypotension.MethodsSixty-six patients were enrolled in a randomized, controlled, quadruple-blinded prospective study into three groups: epidural ropivacaine 0.2% without epinephrine (control) or with 2 µg/mL or 5 µg/mL epinephrine. Our primary outcome was the assessment of differences in hypotension between groups, defined as a >20% decrease in hypotension from baseline to the end of the intraoperative period.ResultsForty-seven patients completed the study, and 19 were withdrawn. Fifteen patients were in the control group, while 16 patients received 0.2% ropivacaine +2 µg/mL epinephrine, and 16 received 0.2% ropivacaine +5 µg/mL epinephrine. The overall rate of hypotension was 21.3% (10/47). There were no statistically significant differences in hypotension rates between the control group (33%) and groups receiving either +2 µg/mL (13%, p=0.165) or +5 µg/mL (19%, p=0.353) of epinephrine. In secondary analyses, respiratory rate showed greater decreases in control groups across the perioperative period compared with treatment groups (p=0.016)ConclusionAdding epinephrine to the epidural local anesthetic did not significantly decrease the rate of hypotension. However, epinephrine mitigated decreases in respiratory rate across the perioperative period. Future studies will focus on increasing group size and higher epinephrine concentrations (10 µg/mL).Trial registration numberNCT02722746.