bluuebunny/crossref_metadata_2025_split huggingface dataset:
HOXB1 restored expression promotes apoptosis and differentiation in the HL60 leukemic cell line
Authors: Petrini Marina , Felicetti Federica , Bottero Lisabianca , Errico Maria Cristina , Morsilli Ornella , Boe Alessandra , De Feo Alessandra , Carè Alessandra 
Published: October 2013

Abstract

Background
Homeobox (HOX) genes deregulation has been largely implicated in the development of human leukemia. Among the HOXB cluster, HOXB1 was silent in a number of analyzed acute myeloid leukemia (AML) primary cells and cell lines, whereas it was expressed in normal terminally differentiated peripheral blood cells.

Methods
We evaluated the biological effects and the transcriptome changes determined by the retroviral transduction of HOXB1 in the human promyelocytic cell line HL60.

Results
Our results suggest that the enforced expression of HOXB1 reduces cell growth proliferation, inducing apoptosis and cell differentiation along the monocytic and granulocytic lineages. Accordingly, gene expression analysis showed the HOXB1-dependent down-regulation of some tumor promoting genes, paralleled by the up-regulation of apoptosis- and differentiation-related genes, thus supporting a tumor suppressor role for HOXB1 in AML. Finally, we indicated HOXB1 promoter hypermethylation as a mechanism responsible for HOXB1 silencing.

Conclusions
We propose HOXB1 as an additional member of the HOX family with tumour suppressor properties suggesting a HOXB1/ATRA combination as a possible future therapeutic strategy in AML.
Randomised controlled trial of a brief alcohol intervention in a general hospital setting
Authors: Shiles Celia J , Canning Una P , Kennell-Webb Sandra A , Gunstone Caroline M , Marshall E Jane , Peters Timothy J , Wessely Simon C 
Published: October 2013

Abstract

Background
The evidence suggests that brief alcohol-focused interventions, directed at hazardous and harmful drinkers in non-specialist settings such as primary care are effective in reducing alcohol consumption. However, there is a need for further research in the hospital setting. This is a randomised controlled trial to investigate the effectiveness of a 10-minute brief intervention amongst 'at risk’ drinkers admitted to general hospital wards. Unlike some previous trials, this trial is randomised, used blinded assessors, includes an intention-to-treat analysis, included female subjects and excluded people with alcohol dependence.

Methods
A total of 250 'at risk’ drinkers admitted to King’s College Hospital were identified using the Alcohol Use Disorders Identification Test (AUDIT). Some 154 subjects entered the study and were randomly allocated to the control and intervention groups. Subjects in the control group received no advice about their drinking whilst subjects in the intervention group received 10 minutes of simple advice on reducing alcohol consumption. Recruitment took place between 1995 and 1997. The primary outcome was the AUDIT questionnaire at 12 months. Secondary outcomes were a previous week’s Drinks Diary, questionnaires (General Health Questionnaire, Alcohol Problems Questionnaire and the Severity of Alcohol Dependence Questionnaire) and laboratory blood tests (gamma glutamyl transferase, mean cell volume and haemoglobin).

Results
At 3-month and 12-month follow-up, all participants were included in the intention-to-treat analysis. At both time points there was no evidence of an intervention effect that could be attributed to the brief intervention. Both the intervention and control groups had an improved AUDIT score and reduced levels of alcohol consumption as measured by a subjective Drinks Diary at 3 months which was maintained at 12 months.

Conclusions
This study has added further evidence on brief interventions in the hospital setting. In contrast to the recent Cochrane review by McQueen et al., the results of this study do not support the effectiveness of a brief alcohol intervention in general hospital wards. However our study was underpowered and there were flaws in the statistical analyses, and these limitations temper the strength of our conclusions.
Syntaxin-4 is implicated in the secretion of antibodies by human plasma cells
Authors: Gómez-Jaramillo Laura , Delgado-Pérez Luis , Reales Elena , Mora-López Francisco , Mateos Rosa M , García-Poley Antonio , Brieva José A , Campos-Caro Antonio 
Published: October 2013

ABSTRACTPCs are responsible for the production and secretion of antibodies, the effector molecules of the humoral immune response. The molecular mechanisms responsible for vesicle docking and secretion implicated in the antibody-secretion process are not well-known, as they have not been studied, but it is known that SNARE proteins are responsible for many membrane-fusion processes in the cell. We show here that freshly isolated human colon LP-PCs and T-PCs from MM-PC patients and the U266 cell line, as a model for PC secretion, contain a set of these proteins. SNAP23, STX3, and STX4 were localized mainly in the plasma membrane of PCs, and interactions of SNAP23 with STX3 and with STX4 were proven by IP. Interaction between SNAP23 and STX4 was also confirmed in situ. With the use of siRNA, as well as shRNA, the functional role of SNAP23, STX3, and STX4 in antibody secretion was also examined. The findings demonstrate that in addition to SNAP23, STX4 is implicated in the antibody secretion by a myeloma cell line and by normal human colon LP-PCs.
Abolition, Race, and the Politics of Gratitude in Late Nineteenth-Century Puerto Rico
Authors: Rodríguez-Silva Ileana M. 
Published: October 2013

Abstract
This essay investigates the political workings of gratitude in Puerto Rico in the postabolition decades in order to uncover how these practices of benevolence, which obscure the violence of everyday marginalization, became key in liberal political forms as a means of rearticulating white superiority and patriarchal authority. The article analyzes the practice of gratitude that liberal elites demanded from former slaves after emancipation as well as the appropriation of and challenges to such practices by laborers. The dynamics explored here appear in a set of performances in newspaper writings and street demonstrations commemorating abolition from the 1870s to the 1890s in the southern city of Ponce, Puerto Rico.
The politics of gratitude refers to the dynamics through which many came to see abolition as an effort to modernize Puerto Rico, an endeavor for which everyone should be morally indebted to abolitionists and their successors. The politics of gratitude thus provided the ideological structures through which liberal reformists could preserve a racialized and patriarchal social order in the absence of slavery. In the process, liberals also constituted themselves as the only intermediaries between popular subjects and the imperial state. Moral indebtedness was one racialized means by which various constituencies sought to craft or accommodate (in the case of authorities) a more inclusive political project that did not contradict the basis of imperial rule—even though it did alter its foundation, if only momentarily, before the US takeover of the island in 1898.
The outcome of total ankle replacement
Authors: Zaidi R. , Cro S. , Gurusamy K. , Sivanadarajah N. , Macgregor A. , Henricson A. , Goldberg A. 
Published: October 2013

We performed a systematic review and meta-analysis of modern total ankle replacements (TARs) to determine the survivorship, outcome, complications, radiological findings and range of movement, in patients with end-stage osteoarthritis (OA) of the ankle who undergo this procedure. We used the methodology of the Cochrane Collaboration, which uses risk of bias profiling to assess the quality of papers in favour of a domain-based approach. Continuous outcome scores were pooled across studies using the generic inverse variance method and the random-effects model was used to incorporate clinical and methodological heterogeneity. We included 58 papers (7942 TARs) with an interobserver reliability (Kappa) for selection, performance, attrition, detection and reporting bias of between 0.83 and 0.98. The overall survivorship was 89% at ten years with an annual failure rate of 1.2% (95% confidence interval (CI) 0.7 to 1.6). The mean American Orthopaedic Foot and Ankle Society score changed from 40 (95% CI 36 to 43) pre-operatively to 80 (95% CI 76 to 84) at a mean follow-up of 8.2 years (7 to 10) (p < 0.01). Radiolucencies were identified in up to 23% of TARs after a mean of 4.4 years (2.3 to 9.6). The mean total range of movement improved from 23° (95% CI 19 to 26) to 34° (95% CI 26 to 41) (p = 0.01). Our study demonstrates that TAR has a positive impact on patients’ lives, with benefits lasting ten years, as judged by improvement in pain and function, as well as improved gait and increased range of movement. However, the quality of evidence is weak and fraught with biases and high quality randomised controlled trials are required to compare TAR with other forms of treatment such as fusion. Cite this article: Bone Joint J 2013;95-B:1500–7.
Improving pre-operative planning for complex total hip replacement with a Rapid Prototype model enabling surgical simulation
Authors: Won S-H. , Lee Y-K. , Ha Y-C. , Suh Y-S. , Koo K-H. 
Published: October 2013

Pre-operative planning for total hip replacement (THR) is challenging in hips with severe acetabular deformities, including those with a hypoplastic acetabulum or severe defects and in the presence of arthrodesis or ankylosis. We evaluated whether a Rapid Prototype (RP) model, which is a life-sized reproduction based on three-dimensional CT scans, can determine the feasibility of THR and provide information about the size and position of the acetabular component in severe acetabular deformities. THR was planned using an RP model in 21 complex hips in five men (five hips) and 16 women (16 hips) with a mean age of 47.7 years (24 to 70) at operation. An acetabular component was implanted successfully and THR completed in all hips. The acetabular component used was within 2 mm of the predicted size in 17 hips (80.9%). All of the acetabular components and femoral stems had radiological evidence of bone ingrowth and stability at the final follow-up, without any detectable wear or peri-prosthetic osteolysis. The RP model allowed a simulated procedure pre-operatively and was helpful in determining the feasibility of THR pre-operatively, and to decide on implant type, size and position in complex THRs.Cite this article: Bone Joint J 2013;95-B:1458–63.
Bibliometric and Social Network Analysis for Data Mining: The Intellectual Structure of Tourism Destination Literature
Authors: Wang Cheng-Hua , Chen Shiu-Chun 
Published: October 2013

Abstract
Data mining can extract useful information from large databases. This paper presents the evolution of the intellectual structure in tourism destination literature as determined by means of bibliometric and social network analysis of 17 552 citations of 414 articles published in Social Sciences Citation Index and Sciences Citation Index journals from 1955 to 2011. This study found that tourism destination research is organized into four different concentrations of interest: destination image, tourist experience and stakeholder involvement, structural equation modeling, and customer relationship management. Future tourism destination research will probably continue to focus on these topics. This study presents a new way for researchers to profile development patterns objectively and provides a key reading method for searching useful research directions.
Homelessness and Money Mismanagement in Iraq and Afghanistan Veterans
Authors: Elbogen Eric B. , Sullivan Connor P. , Wolfe James , Wagner Henry Ryan , Beckham Jean C. 
Published: October 2013

Objectives. We examined the empirical link between money mismanagement and subsequent homelessness among veterans. Methods. We used a random sample of Iraq and Afghanistan War era veterans from the National Post-Deployment Adjustment Survey in 2009–2011. Results. Veterans were randomly selected from a roster of all US military service members in Operation Iraqi Freedom or Operation Enduring Freedom who were separated from active duty or in the Reserves/National Guard. Veterans (n = 1090) from 50 states and all military branches completed 2 waves of data collection 1 year apart (79% retention rate). Thirty percent reported money mismanagement (e.g., bouncing or forging a check, going over one’s credit limit, falling victim to a money scam in the past year). Multivariate analysis revealed money mismanagement (odds ratio [OR] = 4.09, 95% CI = 1.87, 8.94) was associated with homelessness in the next year, as were arrest history (OR = 2.65, 95% CI = 1.33, 5.29), mental health diagnosis (OR = 2.59, 95% CI = 1.26, 5.33), and income (OR = 0.30, 95% CI = 0.13, 0.71). Conclusions. Money mismanagement, reported by a substantial number of veterans, was related to a higher rate of subsequent homelessness. The findings have implications for policymakers and clinicians, suggesting that financial education programs offered by the US Departments of Defense and Veterans Affairs may be targeted to effectively address veteran homelessness.
Cooking, Healthy Eating, Fitness and Fun (CHEFFs): Qualitative Evaluation of a Nutrition Education Program for Children Living at Urban Family Homeless Shelters
Authors: Rodriguez Jacqueline , Applebaum Jo , Stephenson-Hunter Cara , Tinio Andrea , Shapiro Alan 
Published: October 2013

Objectives. We assessed the feasibility of a 15-week nutrition education, physical activity, and media literacy program for children living in urban family homeless shelters. Methods. We developed a qualitative monitoring tool to evaluate program process and impact at 2 shelter sites in the Bronx, New York, from 2009 to 2012. Facilitators recorded indications of participants’ understanding of intended messages and demonstrations of changes in attitudes and behaviors. Comments, insights, and actions were recorded as they occurred. Facilitators also documented barriers to delivery of content and activities as intended. We used content analysis to examine data for patterns and identify themes. Results. A total of 162 children participated at the 2 shelter sites. Analysis of qualitative data yielded 3 themes: (1) children’s knowledge and understanding of content, (2) children’s shift in attitudes or intentions, and (3) interpretations through children’s life experience. Food insecurity as well as shelter food service and policies were important influences on children’s choices, hunger, and sense of well-being. Conclusions. Children’s experiences highlighted the need to advocate for shelter policies that adequately provide for children’s nutritional and physical activity requirements and foster academic development.
Drilling and Completion Fluids Design for Horizontal Well Drilling - Case Histories from Kuwait Field
Authors: Hussein Ahmed , Jadhav Prakash , Sotomayor Ghery , Al-Ajmi Hadi , Al-Ajmi Khaled , Al Ajmi Abdullah , Barazi Nasser Al , Suod Al Rushoud Abdul Aziz , Khan Mohammed Yusuf 
Published: October 2013

Abstract
This paper presents drilling and completion fluids design for drilling long lateral / horizontal wells in a North Kuwait field and its field application on well RA-AAA, RA-BBB, RA-CCC and RA-DDD. The offset wells were reviewed to identify the issues from drilling a challenging trajectory through troublesome reactive formations that showed serious wellbore stability issues and stuck pipe incidents.
The customized drilling and completions fluids system was designed for different intervals, taking the following objectives in consideration: Improved hole stability through stressed and reactive formationsEnhanced hole-cleaning efficiency at critical anglesMinimized risk of stuck pipe across depleted formations with high porosity and permeabilityMinimized / no induced losses to formation by utilizing unique wellbore-strengthening techniqueMinimal damage to reservoir section during drilling phaseNear-wellbore damage remediation during completion phase
This paper describes the customized drilling and completions fluids performance as compared to offset wells. A comprehensive engineered approach addressed the challenges of drilling horizontal wells by using a revolutionary bridging technology to strengthen the wellbore and improve wellbore stability to reduce non- productive time (NPT) related to losses, stuck pipe, etc. This paper also describes the completions fluids design to minimize reservoir damage and utilize Mesophase technology to remediate near-wellbore damage and improve reservoir producibility. The lessons learned on these wells were incorporated in drilling subsequent wells to continue improve on performance.
Prediction Model of Oil Economic Limit Production and Its Comparative Study
Authors: qing Xu , shuqin Che , haiyan Meng , min Zhong , jinying Wang 
Published: October 2013

Abstract
The Enterprise Standard of Method for Petroleum Initial Reserves Evaluation in China prescribes the economic limit production prediction model is the standard evaluation method of the developed oil and gas initial reserves. This model is derived from the theory of input-output balance, and the important key is the clear classification of fixed cost and variable cost in use of this model. Owing to the composite of operating cost, it is difficult to divide the operating costs into fixed costs and variable costs, therefore affects the evaluation accuracy of the initial reserves to a certain extent. Due to more and more oilfields entering the middle to later development stage, the measures of increasing production and the development mode change become the important ways for holding and increasing production, thus cause the differences of cost structure in different stages, therefore the difficulty of cost classification and the uncertainty of initial reserves evaluation are increasing obviously.
In order to avoid the cost classification errors and evaluation effects of proved recoverable reserves, this paper sets up an economic limit of oil production prediction model derived from the discounted cash flow method. Furthermore, this paper puts the prediction model and Petrochina Standard’s model and D&M Co.’s simple calculating formula comparison. The comparison study shows several differences among the three models on formula derive basis, cost preferences and government revenues. Cases analysis shows important conclusions as follows, first, the cost classification was not involved in model calculation process; thereby multiple solutions from the economic limit production calculation were avoided effectively. Second, the model standardizes the costs preference and its assignment, and then improves the economic limit prediction accuracy. Third, the formulas determined by this model can conveniently complete the scenario analysis of the oil price, cost, government revenue and other factors.
Real-Time Surveillance - How System Integration Minimises Deferment, Optimises Production, Maximises Test Unit Capacity and Tracks Well Operating Envelopes
Authors: Al Balushi Mahmood , Konwar Anupam , Abdullah Zulfikri , Sahu Sambit-Kumar , Briers Jan 
Published: October 2013

Abstract
A key issue in upstream E&P operations is how to manage wells and reservoirs more effectively. Oil and gas production from onshore and offshore wells can be relatively difficult to manage, not only because of multiphase nature of the well production, but also due to lack of a continuous, reliable measurement of well/reservoir performance. Reporting continuous production from the wells and performing hydrocarbon allocation with credible accuracy is an area with many challenges in upstream E&P operations.
Shell's Production Universe (PU) is a novel software application developed by Shell to address some fundamental gaps in the management and surveillance of oil and gas production systems. Using data driven models, it is designed to estimate and optimize the production (and injection) of wells continuously in real-time, based on the available wellhead instrumentation. PU provides improved well surveillance, more accurate hydrocarbon accounting, and automatic production reporting and production optimization. The data from PU can also be used for quick analysis to safeguard the technical integrity of wells and reservoirs. It is also cost-effective in that it requires minimal commodity instrumentation and IT systems, much of which may already be present in field operations.
Brunei Shell Petroleum (BSP) in South East Asia had adopted the PU technology just after it was launched in 2004. Today, BSP continues to deploy PU in all of its assets. Implementation of PU in BSP has helped in exception based surveillance, analysis and troubleshooting; and hence has helped optimized resources being directed towards production optimization activities. It has also improved data quality and reporting.
This paper discusses how Production Universe has been instrumental in helping BSP in the above operational and production areas. In particular, the application of PU in various facets of WRFM (Wells, Reservoir and Facility Management) to achieve operational excellence is discussed.
Expanding Cement Application for High Rate Gas Wells in South Sumatra
Authors: Sutama Candra , Inayah Firda 
Published: October 2013

Abstract
The cement sheath of an oil or gas well is designed to prevent fluid communication between the drilled formations during the productive life of the well through post-abandonment. However, even when cement initially provides good hydraulic seal, zonal isolation can deteriorate after initial placement due to microannuli formation. In many cases, a microannulus can be formed because of wellbore temperature changes, wellbore pressure changes, or set cement bulk shrinkage.
Microannuli are considered serious concern in gas wells because, unlike liquid, gas flows easily to surface through very narrow microannuli. Cementing operations which fail to provide adequate zonal isolation may result in loss of isolation which poses a hazard to both personnel and the environment. A cement that has ability to expand post set, can help prevent the formation of microannuli. The concept is that the expanding cement will fill microannuli and ensure good bonding either between the formation and the cement or between casing and the cement.
This paper discusses the application of expanding cement in two large gas fields; located in South Sumatra. In those fields, wells are produced using 7- in production tubing, with 13 3/8-in casing and 9 5/8 in liner as production strings. Production strings are constantly exposed to changes in pressure and temperature, both during drilling and during production. These changes can create stresses on the cement sheath and may create microannuli. There is a particular focus on the isolation around 13 3/8- in production string: unlike the 9 5/8-in production liner string, the 13 3/8-in string relies only on cement to isolate the gas zones, without any packer. This string needs to provide isolation is critical to ensure that the well can perform as designed and pose no hazard to personnel and to the environment. The paper also discusses the design steps and considerations, as well as evaluation results.