bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heart Failure Recurrence Prevention: Patient Knowledge, Attitudes, and Practices in Yancheng City
Authors: He Yanhong , Dong Zhifeng , Gu Xingjun , Xu Jiaoyan , Li Xinli 
Published: August 2024

Abstract

To investigate the knowledge, attitudes, and practices (KAP) concerning the prevention of heart failure recurrence among patients. A cross-sectional study was conducted at the Third People's Hospital of Yancheng City from September 1, 2023, to December 31, 2023, employing a self-administered KAP questionnaire. The study included 421 heart failure patients, of whom 230 (54.6%) were male. Median scores for knowledge, attitudes, and practices were 12.00 [10.00, 13.00] (possible range: 1-18), 20.00 [19.00, 21.00] (possible range: 5-25), and 16.00 [15.00, 17.00] (possible range: 5-22), respectively. Pearson’s correlation analysis indicated a positive correlation between knowledge and attitude scores (r=0.183, P<0.001), as well as between knowledge and practice scores (r=0.169, P<0.001). A higher attitude score was significantly associated with a higher practice score (r=0.245, P<0.001), as supported by structural equation modelling. Multivariate logistic regression revealed that attitude score (OR = 1.238), suburban residence (OR = 2.524), monthly household income exceeding 5000 Yuan (OR = 3.539), BMI less than 20 kg/m2 (OR = 2.155, P = 0.029), BMI between 20-24 kg/m2 (OR = 2.387), and BMI between 25-29 kg/m2 (OR = 4.063) were independently linked to proactive practices (all P<0.05). Additionally, Structural Equation Modelling (SEM) elucidated the intricate relationship between sociodemographic factors and KAP dimensions. Despite positive attitudes and proactive prevention efforts, heart failure patients exhibit a knowledge gap. Tailored educational interventions by healthcare providers could effectively bridge this gap, leveraging patients' positive attitudes and proactive behaviours to reinforce preventive measures.
Two cases of Lowe syndrome caused by a novel mutation in OCRL gene in a family
Authors: Huang Yuchan , Wang Le , Wang Xinlei , Sun Xiaoying , Han Peitong , Wang Yuxue , Li Chunzhen 
Published: August 2024

Abstract

Background
Lowe syndrome is a rare X-linked recessive genetic disease characterized by congenital binocular cataracts, central nervous system developmental delay, and progressive renal failure caused by tubular injury. Mutations in the OCRL gene can lead to two diseases: Dent-2 syndrome and Lowe syndrome.
Case introduction: Two boys from a family have similar clinical manifestations, including congenital bilateral cataracts, mild developmental abnormalities, and low molecular weight proteinuria. Through whole exome sequencing, it was found that both boys had missense mutations on exon 21 (c.2302A > T, p.Ile768Phe), a newly discovered mutation site.
Conclusion
The discovery of Lowe syndrome in a family of children should be taken seriously, and genetic validation should be conducted on the remaining children in the family as soon as possible. Early intervention and treatment of the disease should be carried out to delay the progression.(This case report is a retrospective study. The parents of the patient have signed an informed consent form and agreed to publish the report. The clinical trial has not been registered.)
Application of Digital Virtual Reality Simulator for Dental Surgical Skill Training
Authors: Li Rui-fang , Ren Jian-gang , Zhang Wei , Yu Zi-li , Chen Gang 
Published: August 2024

Abstract

Aim
Dental surgical skills training assumes critical importance for aspiring dentists. Our present study aimed to investigate the effectiveness of innovative DVRS approach in preclinical dental surgical skill training.
Methods
A total of 142 students participated in this study. Within this cohort, 70 students from the 2017 intake were trained using traditional manikins (Group TM), while 72 students from the 2018 were trained using a Digital Virtual Reality Simulator for Dental Surgical Skills (Group DVRS). The scores of the training course between two groups were compared. Additionally, the experiences of students from Group DVRS were collected through a questionnaire survey regarding the use of DVRS in their preclinical training.
Results
The comparative analysis revealed that the average scores for Group DVRS were slightly higher than Group TM (P < 0.05). The feedback collected via questionnaires from Group DVRS participants reflected their satisfaction with the DVRS, praising its user-friendliness, clarity, training efficacy, and the resultant enhancement in their operational skills.
Conclusions
These findings suggest that DVRS is an effective and beneficial tool for preclinical dental surgical skill training.
Assessing the long-term care (LTC) service needs of older adults based on Time-Driven Activity-Based Costing (TDABC)——A cross-sectional survey in central China
Authors: Fang Li , Li Li , Huang Weihong , Zeng Yuting , Long Yanfang , Peng Jing , Hu Jianzhong , Li Jing , Chen Xi 
Published: August 2024

Abstract

Background
The global population's aging has led to an increasing demand for long-term care (LTC), yet a significant gap exists between the high demand for LTC and the low availability of services, especially in developing countries like China. Comprehensive assessment of LTC service demands by including the time and cost analysis is crucial to inform the planning and financing of LTC resources, yet such research is lacking in China.
Methods
A cross-sectional study was conducted in Changsha City, Hunan Province, China, from June 2021 to December 2022. A stratified multistage cluster sampling method was used to recruit 1,270 older adults from five nursing homes and three communities/streets in three regions of Changsha City. The LTC service needs were assessed by calculating the service time required from caregivers, nurses, and doctors and the total service time (min/d). Participants' disability, activities of daily living (ADLs), mental status and social involvement (MSSI), and sensory and communication (SC) were assessed using standard scales. Generalized linear regression models were used to analyze factors associated with LTC service needs.
Results
The participants had an average age of 76.41 ± 8.38 years, with 43.7% being female. The median service time required from caregivers, nurses, and doctors was 53.34 min/d, 3.66 min/d, and 0.33 min/d, respectively, and the total service time was 83.31 min/d. The generalized linear regression model identified the following factors that were associated with higher total service time: Aged 75–84 years, Living in nursing homes, Income over 5000 per month, ADLs, MSSI (9 ~ 40), SC and having 3 ~ 4 kind comorbidity of geriatric (P < 0.05).
Conclusions
Older adults have a high need for LTC services, especially those provided by caregivers, indicating an urgent need to expand and improve LTC systems. The LTC service time needed is associated with multilevel factors encompassing sociodemographic, functional, and psychological aspects. This study assess the service time required of older adults and their influencing factors based on Time-Driven Activity-Based Costing (TDABC) and offer deeper insights into the needs, demands, and costs of LTC services for older adults and provide essential guidance for future planning and financing of LTC resources.
Effect of butyrate, a short-chain fatty acid, in repairing brain injury in neonatal rats
Authors: Zhao Jing , Feng Zhiyuan , Yang Can , Zhang Jun , Jiang Lin , Hou Li 
Published: August 2024

Abstract

Butyrate, as a microbial metabolite, is considered to have an effect on improving the intestinal microenvironment. At present, it is shown that the brain and gut interact with each other through the gut-microbiota-immune-brain axis. An aberrant gut-microbiota-immune-brain axis in premature infants may aggravate brain injury. However, whether sodium butyrate can improve the microbial-intestinal-brain axis to repair the brain injury in premature infants remains unclear. In this study, we established a neonatal rat hypoxic-ischemic brain injury model and a necrotizing enterocolitis model. It was found that enteritis could lead to the occurrence and aggravation of brain injury, which might be associated with the increased secretion of inflammatory factor interleukin-17 caused by the down-regulation of suppressor of cytokine signaling 1 (SOCS1). Further studies showed that sodium butyrate can up-regulate the expression of SOCS1, and increase the secretion of anti-inflammatory interleukin-10, which may alleviate the neurological dysfunction caused by brain injury. This study provides a new theoretical basis for further exploration of the mechanism of brain injury repair in premature infants.
A Synthesis of Metal-Organic Frameworks Using Terephthalic Acid Obtained from PET Bottles with Dye Adsorption for Application in Experimental Chemistry Classes
Authors: Gonçalves João Manoel R. , Brandão Júlia V. , Lima-Neto Benedito S. 
Published: August 2024

Abstract

In this study, we synthesized Metal Organic Frameworks (MOFs) using five transition metals from the 3d series: iron, cobalt, nickel, copper, and zinc. The ligands employed were Terephthalic Acid, also known as H₂TPA (benzene 1,4-dicarboxylate acid), recovered from PET bottles. The process of obtaining H₂TPA involved alkaline hydrolysis of PET bottle fragments in a 10 mol·L⁻¹ NaOH solution (40% m/w) placed under reflux. Once H₂TPA was obtained, it could be mixed with transition metal acetates. When these mixtures were combined in N,N’-dimethylformamide (DMF) solutions, the formation of metallic MOFs was observed. Some characteristics of the MOFs, particularly their adsorption capacity, were demonstrated through UV-Vis absorption spectroscopy using solutions of common laboratory acid-base indicator dyes such as Methylene Blue (NB), Methyl Orange (MO), Nile Blue (NB), and Malachite Green (MG). This type of simple experiment can be applied in undergraduate Chemistry classes, involving synthesis, characterization, and applications of MOFs compounds extensively documented in scientific literature. Furthermore, the use of the H₂TPA ligand through chemical recycling adds an environmentally friendly aspect to the experiments, resulting in low-cost, easily executable procedures that align with green chemistry principles.
Unusual Inclusions And Genesis Of Microdiamonds From The Zelenyi Yar Neogene Placer (The Ukrainian Shield): A Tem Study
Authors: Kvasnytsya Victor M. , Wirth Richard , Vyshnevskyi Oleksiy A. , Schreiber Anja 
Published: August 2024

Abstract

16 individual diamond microcrystals from the Neogene placer of Zeleny Yar on the Ukrainian Shield have been studied using TEM methods. Placer sediments are fine-grained sands of coastal marine origin redeposited in a river valley. The placer heavy minerals include ilmenite, rutile, zircon, kyanite, sillimanite, staurolite, tourmaline, mantle and impact type diamonds. Undisputed minerals genetically related with mantle diamond were not found in the placer. The studied mantle diamonds have different morphologies: octahedron, octahedroid, octahedron-rhombic dodecahedron transition crystal, cube, cuboid, crystals of {100} + {111} + {110} combinational form, fragments of octahedra. The size of the crystals does not exceed 0.5 mm. They differ in color - from colorless to brown and gray, and growth mechanisms - tangential for octahedra and transitional crystals and fibrous for cubic crystals. Some optically clear diamond crystals contain distinct dark inclusions already visible under a binocular microscope. 21 TEM foils 15 × 10 × 0.150 µm in size each, prepared from typical crystals, have been studied. Two groups of diamonds are distinguished based on inclusions: 2 diamonds with sets of inclusions typical for diamonds from ophiolites and the remaining 13 crystals with sets of multiphase inclusions and structural defects typical for diamonds from kimberlites, lamproites and lamprophyres. Crystals of the first group are cube-octahedron and a fragment of an octahedron. Their inclusion are: Mn-olivine, MnNi alloy with minor Si content, MnNiO3 oxide, Mn5C2 carbide, rhodonite (Mn(SiO3) and amorphous silica. MnNiO3 oxide is associated with silica and chlorine. Most of the second group crystals are characterized by curved dislocations, carbon platelets were found in several crystals and nitrogen platelets in one crystal. Inclusions in these diamonds are: dolomite, ilmenite, magnetite, apatite, mica, chlorite and orthopyroxene. One crystal contains a Fe-rich forsterite inclusion. They belong to the typical eclogite assemblages of mantle diamond from kimberlites. Crystals of this group experienced significant annealing. Possible original sources for crystals of the first group are proposed serpentenites, for second group - kimberlites or lamproites or lamprophyres.
In Vitro and Ex-Vivo Direct Effects of Coagulation Factors on on Bone Cells: Exploring the Bone Remodeling Alterations in Haemophilia A
Authors: Battafarano Giulia , Lancellotti Stefano , Sacco Monica , Rossi Michela , Terreri Sara , Gregorio Jacopo Di , Giuseppe Laura Di , D’Agostini Matteo , Porzio Ottavia , Gennaro Leonardo Di , Tardugno Maira , Pelle Simone , Minisola Salvatore , Toniolo Renato Maria , Luciani Matteo , Fattore Andrea Del , De Cristofaro Raimondo 
Published: August 2024

Abstract

Haemophilia is associated with reduced bone mass and mineral density. Due to the rarity of the disease and the heterogeneity among the studies, the pathogenesis of bone loss is still under investigation. We studied the effects of coagulation factors on bone cells and characterized in a pilot study the osteoclastogenic potential of patients’ osteoclast precursors. To evaluate the effect of coagulation factors on osteoclasts, we treated Healthy Donor-Peripheral Blood Mononuclear Cells (HD-PBMC) with Factor VIII (FVIII), von Willebrand Factor (VWF), FVIII/VWF complex, activated Factor IX (FIXa), activated Factor X (FXa) and Thrombin (THB). FVIII, VWF, FVIII/VWF, FXa and THB treatments reduced osteoclast differentiation of HD-PBMC and VWF affected also the bone resorption. Interestingly, PBMC isolated from patients with moderate/severe haemophilia showed an increased osteoclastogenic potential due to the alteration of osteoclast precursors. Moreover, increased expression of genes involved in osteoclast differentiation/activity was revealed in osteoclasts of an adult patient with moderate haemophilia. Control osteoblasts treated with the coagulation factors showed that FVIII and VWF reduced ALP positivity; the opposite effect was observed following THB treatment. Moreover, FVIII, VWF and FVIII/VWF reduced mineralization ability. These results could be important to understand how coagulation factors deficiency influences bone remodeling activity in haemophilia.
Common Mycorrhizal Networks Improve Survival and Mediate Facilitative Plant Interactions among Andropogon gerardii Seedlings Under Drought Stress
Authors: Russell Margaret , Řezáčová Veronika , Miller Kirby Shane , Nardi Wynter Helene , Brown Morgan , Weremijewicz Joanna 
Published: August 2024

Abstract

Under drought conditions, arbuscular mycorrhizal (AM) fungi may improve plant performance by facilitating the movement of water through extensive hyphal networks. When these networks interconnect neighboring plants in common mycorrhizal networks (CMNs), CMNs are likely to partition water among many individuals. The consequences of CMN-mediated water movement for plant interactions, however, are largely unknown. We set out to examine CMN-mediated interactions among Andropogon gerardii seedlings in a target-plant pot experiment, with watering (watered or long-term drought) and CMN status (intact or severed) as treatments. Intact CMNs improved the survival of seedlings under drought stress and mediated positive, facilitative plant interactions in both watering treatments. Watering increased mycorrhizal colonization rates and improved P uptake, particularly for large individuals. When drought stressed, improved access to water most likely benefited neighboring plants interacting across CMNs. CMNs appear to have provided the most limiting resource within each treatment, whether water, P, or both, thereby improving survival and growth. Neighbors near large, photosynthate-fixing target plants likely benefited from their establishment of extensive hyphal networks that could access water and dissolved P within soil micropores. In plant communities, CMNs may be vital during drought, which is expected to increase in frequency, intensity, and length with climate change.
Large-cell neuroendocrine carcinoma of the bladder: A case report
Authors: Zhou Yu , Yang Lin 
Published: August 2024

Abstract

BACKGROUND
Bladder neuroendocrine tumors are few and exhibit a high degree of aggressiveness. The bladder is characterized by four distinct forms of neuroendocrine tumors. Among them, large-cell neuroendocrine carcinoma is the least prevalent but has the highest level of aggressiveness. To date, only a few dozen cases have been reported.
CASE SUMMARY
Here, we report the case of a 65-year-old man with large-cell neuroendocrine carcinoma of the bladder. The patient presented to the Department of Urology at our hospital due to the presence of painless hematuria without any identifiable etiology. During hospitalization, abdominal computed tomography revealed the presence of an irregular mass on the right anterior wall of the bladder. A cystoscopic nonradical resection of the bladder lesion was performed. The postoperative pathological examination revealed large-cell neuroendocrine bladder cancer.
AnReal: Towards an extended reality, low-cost system for kinesiophobia
Authors: Herskovic Valeria , Arredondo Diego , Torres Gustavo , Vidal Catalina , Campos Mauricio 
Published: August 2024

Abstract

Background
Virtual reality (VR) has been successfully used as part of treatment for patients with chronic low back pain and kinesiophobia. However, the cost of VR systems is still too high to be used in interventions in low-income countries, and systems may produce adverse effects on patients, such as cybersickness and reduced sense of presence.
Objective
This study presents a novel mobile-based extended reality (XR) system that combines views of the real world with a simulated video trajectory that aims to be affordable, provide a good user experience, and provide the essential elements to treat kinesiophobia.
Methods
This paper presents the concept of the system, which is the first system aimed at reducing kinesiophobia that uses XR, as well as its development process and an initial evaluation in physical therapy students.
Results
The system was found to provide an acceptable user experience, but high cybersickness values as indicated by a score of 31.52 on the SSQ questionnaire.
Conclusions
This research proposes and implements a novel, low-cost alternative for treating kinesiophobia related to chronic pain, representing the first system for these purposes that uses extended reality instead of virtual reality.1
Role and screening potential of let-7b-5p, miR-15a-5p, and miR-15b-5p in human prostate cancer
Authors: Sun Chen , Li Xutai , Lu Chong , Wen Zhenyu , Ge Zhenjian , Chen Wenkang , Li Yingqi , Zhou Huimei , Wu Yutong , Lin Shengjie , zhang Pengwu , Wang Wuping , Chen Siwei , Li Hang , Tao Lingzhi , Wu Xionghui , Lai Yongqing 
Published: August 2024

Abstract

Prostate cancer is the fifth leading cause of male cancer mortality and poses a serious threat to men's health worldwide. PSA testing is widely used in prostate cancer screening, but its high false-positive rate leads to unnecessarily high subsequent testing costs, mental anguish and potential physical harm to patients. Therefore, there is an urgent need to develop a convenient, cost-effective and non-invasive diagnostic method to assist in reducing the false-positive rate of PSA screening. The aim of this study was to evaluate the diagnostic value of serum MicroRNA expression in patients with prostate cancer. We selected 10 miRNAs in the literature that were associated with prostate cancer. Afterwards, we measured the expression levels of these miRNAs in serum of 112 prostate cancer patients and healthy controls through a training phase and a validation phase. By plotting receiver operating characteristic curve, the miRNAs with the highest diagnosis value were chosen. Then, a set of miRNAs with the top diagnostic value was identified using stepwise logistic regression. The findings showed that 5 kinds of miRNAs (let-7b-5p, miR-15a-5p, miR-133a-3p, miR-15b-5p, miR-144-3p) were abnormally expressed in the serum of prostate cancer patients. The diagnostic panel constructed with these 3 miRNAs including let-7b-5p, miR-15a-5p miR-15b-5p and which have high specificity and sensitivity in detecting prostate cancer (area under the curve (AUC) = 0.899). Our study illustrates the potential of a three-microRNA panel in the diagnosis of prostate cancer, which can help in the early detection of prostate cancer and may even assist in reducing the false-positive rate of PSA screening.
Constructing a Neutrophil Extracellular Trap Model Based on Machine Learning to Predict Clinical Outcomes and Immunotherapy Response in Renal Cell Carcinoma
Authors: Zhu Yihao , Li Yajian , Li Xuwen , Yu Yuan , Chen Can , Wang Mingshuai , Chen Dong , Xing Nianzeng , Yang Feiya , Ye Xiongjun 
Published: August 2024

Abstract

Neutrophil extracellular traps (NETs) represent a novel form of inflammatory cell death within neutrophils. Emerging research indicates that NETs promote cancer progression and metastasis in various ways. This study aims to provide prognostic NETs characteristics and therapeutic targets for patients with renal cell carcinoma (RCC). NMF analysis was conducted on 89 NET-related genes in the training cohort. Subsequently, WGCNA networks were utilized to study the subtype feature genes. Six machine learning algorithms were assessed for model training, and the optimal model was selected based on 1-year, 3-year, and 5-year AUC values. A NETs signature was then constructed to predict overall survival in RCC patients. Furthermore, multi-omics validation was performed based on NETs signature. Finally, stable knockout key gene RCC cell lines were established to verify the biological function of KCNN4 both in vitro and in vivo. This study highlights the emerging hot topic of NETs in RCC. We provide a prognostic NETs signature and identify multiple roles of KCNN4 in RCC. This work contributes to risk stratification and the identification of new therapeutic targets for RCC patients.