bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunotherapy of Melanoma: Current Status and Prospects for the Future
Authors: Irie Reiko F. , DeNunzio F. David 
Published: April 2015

AbstractAs one of the most immunogenic human malignancies, melanoma has served as a prototype for the development of immunotherapy against human cancer in a number of treatment modalities over the past two decades. These studies have included augmentation of host immunity with lymphokines and other biological response modifiers, lymphokine‐activated killer cells, and cytotoxic T‐cells; active immunotherapy using purified antigens, tumor cell vaccines, and anti‐id antibodies; and passive immunotherapy with monoclonal antibodies. The results of these investigations are discussed as well as the prospect for future treatments of melanoma incorporating immunotherapeutic techniques. A report on a recent trial of gene therapy of human melanoma performed by Dr. Steven Rosenberg is also presented.
Effects of Ultraviolet‐B and PUVA on Ornithine Decarboxylase Activity, DNA Synthesis, and Protein Kinase C Activity in Mouse Skin
Authors: Mizuno Nobuyuki , Kono Takeshi , Taniguchi Shoji , Fukuda Michio , Maekawa Naoki , Hisa Tomoyuki , Otani Shuzo , Hamada Toshio 
Published: April 2015

AbstractUltraviolet‐B and PUVA share several biological events with phorbol ester tumor promoters. The effects of ultraviolet‐B irradiation and topical PUVA treatment on ornithine decarboxylase activity, DNA synthesis, and protein kinase C activity, which are known to be induced or activated by phorbol ester tumor promoter, were investigated in hairless mouse skin. Ornithine decarboxylase activity was remarkably enhanced by ultraviolet‐B and PUVA. Although PUVA did not affect DNA synthesis significantly, ultraviolet‐B stimulated epidermal DNA synthesis approximately 5‐fold over control values at 48 h. However, unexpectedly, neither cytosolic nor membrane‐bound protein kinase C activity showed any change during the 2 h after either treatment. These results suggest that the protein kinase C system is not involved in the initial signal transduction system of ultraviolet‐B or PUVA, unlike the case with phorbol ester tumor promoter.
Generalized Eruptive Histiocytoma: Report of a Pediatric Case
Authors: Izaki Seiichi , Kitamura Keijiro , Arai Eiichi 
Published: April 2015

AbstractA one‐year‐old boy was seen with brownish, flat papules on his face, neck, and upper arms. The lesions were symmetric, discrete, slightly raised, firm to the touch, and asymptomatic papular eruptions. Histological and immunohistochemical investigation revealed monomorphous infiltration of S‐100 negative, lysozyme negative, and α1‐antitrypsin negative, but α1‐antichymotrypsin positive and vimentin positive histiocytic cells with small numbers of lymphocytic cells. The histiocytic cells did not show any foamy changes in the cytoplasm. No giant cells were found. The lesions increased in number during the first few years and then spontaneously regressed by the sixth year of age, leaving partial brownish pigmentation.
Keratoacanthoma with Glandular Proliferation
Authors: Akiyama Masashi , Hata Yasuki , Nishikawa Takeji 
Published: April 2015

AbstractA case of keratoacanthoma (KA) with glandular proliferation was reported. The tumor was a firm, dome‐shaped, elevated nodule on the cheek of an 82‐year‐old Japanese male. Generally, the tumor showed the typical histopathological features of KA; slight nuclear atypia and mitotic figures were present in a cup‐shaped proliferation of keratinocytes and, in the center of the lesion, a keratin‐filled crater with nests of dyskeratotic and acantholytic cells was seen. In the bottom of the lesion, a glandular structure forming branching, thin lumina was observed. The epithelium of the lumina was made up of two or more layers of columnar or cuboidal cells without keratinization. Tall columnar cells with oval nuclei were located in the luminal row and small cuboidal cells with round nuclei and scanty cytoplasm were in the outer layer. Immunohistochemical staining revealed that only the glandular structure was carcinoembryonic antigen positive. Lectin binding patterns observed in the glandular proliferation were similar to those reported for the apocrine gland. KA sometimes exhibits an adenoid structure in its lesion because of acantholytic changes. However, KA associated with true glandular proliferation has not been reported as far as we know, and our case is the first reported one. KA is thought to be a tumor of follicular origin, and the glandular proliferation observed in the present case seemed to have certain characteristics that suggest its apocrine origin.
Dystrophic Calcinosis of the Penis
Authors: Katoh Norito , Okabayashi Keiko , Wakabayashi Shunji , Kishimoto Saburo , Yasuno Hirokazu 
Published: April 2015

AbstractA 22‐year‐old man had had a dome‐shaped tumor on his penis for one year. Neither his particular past history nor family history was available. The excised specimen contained numerous von Kossa‐positive deposits. Four types of histologic pattern were identified: 1) a cystic structure filled with amorphous material lined by a few layers of epithelial cells, 2) a cyst containing calcified deposits in the keratinous material, 3) a large calcified nodule lined by attenuated epithelial cyst walls, 4) numerous calcium collections without an epithelial wall. The cystic structure showed the histologic features of syringoma or sweat duct milia. The luminal cells of the cyst showed positive immunoreactivity for both keratin and carcinoembryonic antigen. These findings suggested that the keratinous contents of syringoma had gradually calcified, the cyst wall had been attenuated, and, finally, numerous calcium collections without an epithelial wall were formed. Our case further supported the hypothesis that penile calcinosis as well as scrotal calcinosis might derive from syringoma.
Medullasin Levels in Neutrophils of Patients with Pustulosis Palmaris et Plantaris
Authors: Nakamura Takehiko , Ono Tomomichi , Aoki Yohsuke 
Published: April 2015

AbstractMedullasin is one of the serine proteases found in human bone marrow cells. The protease in granulocytes is assumed to play an important role in the induction of chronic inflammation.We measured the medullasin levels as a parameter of neutrophil function in granulocytes from patients with pustulosis palmaris et plantaris. All 17 patients studied underwent tonsillectomies, and the changes in the amount of medullasin were followed after surgery. Seven of the 17 patients were cured during the follow‐up period (average: 21 months). In all the patients in whom PPP improved, the medullasin levels decreased significantly from the period of active disease when pustules were present to the inactive period. The amount of medullasin did not fall significantly in patients whose PPP did not improve. It is concluded that medullasin levels may reflect the activity of PPP and may serve as a criterion for performing a tonsillectomy.
Pharmacokinetics of Meropenem in Experimentally Burned Rats
Authors: Yoshida Tetsunori , Homma Ken‐ichi , Azami Kenji , Sugihara Tsuneki , Ohura Takehiko 
Published: April 2015

AbstractThe pharmacokinetics of meropenem were investigated to examine its penetration into the skin of third degree burned rats. The rats were divided into two groups. One group acted as the control, and the other group had third degree burns induced by immersing their backs into 80°C water for 20 seconds. The rats in each group were given 20 mg/kg of body weight of meropenem intravenously by one bolus injection seven days after burn inducement or depilation. In the non‐burned control group, the maximum concentrations of meropenem in the serum and skin of 6.03 µg/ml and 0.12 µg/g were respectively obtained 15 minutes after the injection and decreased very rapidly thereafter. In the burned rats, the maximum concentrations of meropenem in the serum, skin (eschar), and exudate fluid of 7.07 µg/ml, 1.44 µg/g and 5.99 µg/ml were respectively obtained 15 minutes after the injection and decreased very slowly. The penetration of meropenem into the burned skin was higher than that into the normal skin. These results suggest that meropenem is a very useful antibiotic in the treatment of burn infection.
Two Sisters with Leprosy
Authors: Ishii Norihisa , Sugita Yasuyuki , Nakajima Hiroshi 
Published: April 2015

AbstractLeprosy is a rare entity in Japan, but remains quite common in developing countries. We report two sisters with leprosy from Brazil but currently working in Japan who presented to our clinic. The younger sister was infected with the BB type with HLA‐A2, A24, B51, Bw52, DR2, DRw8, DRw52, DQwl, and DQw3. The elder sister had the TT type with HLA‐A2, A31, B51, Cw3, DRw6, DRw8, DRw52, DQwl, and DQw3.Immunohistochemical findings revealed that CD4+, 4B4+ helper/inducer T‐cells were dominant in the granulomas of both cases. Bacilli were not detectable in the biopsy specimens. However, Mycobacterium leprae‐specific DNA fragments were found in their peripheral blood and biopsy specimens by polymerase chain reaction.
Chédiak‐Higashi Syndrome: Report of a Case and Review of the Japanese Literature
Authors: Fukai Kazuyoshi , Ishii Masamitsu , Kadoya Atsuko , Chanoki Miyako , Hamada Toshio 
Published: April 2015

AbstractWe report the case of a Japanese female infant with Chédiak‐Higashi syndrome born to consanguineous parents. At birth she had fair skin but, when she was three months old, marked hyperpigmentation of the sun‐exposed skin areas developed. Microscopic examination of blood and electron microscopic examination confirmed the diagnosis. She enjoyed good health until she was two years old when she had pneumonia with marked hepatosplenomegaly. It is important for dermatologists and pediatricians to be aware of the skin manifestations of this disease because hyperpigmentation after sun exposure may be a characteristic, initial feature of this condition.
Chronic Graft‐versus‐Host Disease with Follicular Involvement
Authors: Miyazaki Katsuko , Higaki Shuichi , Maruyama Tomohiro , Takahashi Shozo , Morohashi Masaaki , Ito Kaoru 
Published: April 2015

AbstractThe clinical and histopathological findings in a 25‐year‐old Japanese male patient who suffered from chronic graft‐versus‐host‐disease (GVHD) with follicular involvement are described. The patient had been diagnosed as aplastic anemia and underwent an allogeneic bone marrow transplant (BMT). In the eighth month thereafter, pruritic follicular red papules developed over his trunk and extremities. A biopsy specimen revealed histological exocytosis of lymphocytes into the hair follicles and basal‐cell vacuolization of the follicular epithelium. Analysis of T‐lymphocyte subpopulations in the dermis revealed a predominance of CD4 positive cells. To our knowledge, several cases of acute follicular GVHD have been reported (1, 2); however, the occurrence of chronic GVHD with follicular involvement (chronic follicular GVHD) has not been clearly documented.
Angiokeratoma Corporis Circumscriptum Naeviforme with Transepidermal Elimination
Authors: Miwa Norimasa , Kobayashi Taisuke , Kanzaki Tamotsu , Tsuji Takuo 
Published: April 2015

AbstractA 5‐year‐old Japanese boy visited our clinic for treatment of a dark bluish papulonodular lesion on his right thigh. The histopathology of the lesion indicated angiokeratoma corporis circumscriptum naeviforme. Topical antibiotics were prescribed for occasional bleeding from the site. Frequent bleeding started in April of 1992, when erythema and itching were observed around the angiokeratoma. Histopathology of the lesion showed heavy lymphoid cell infiltration around dilated capillaries and irregular acanthosis engulfing dilated capillaries. Interestingly, there were confined masses of red blood cells in the epidermal layer as well as both in and on the horny layer. This is a feature of transepidermal elimination. This transepidermal elimination appeared to be triggered or enhanced by the concomitant contact dermatitis due to topical antibiotics. To the best of our knowledge, this is the first report of angiokeratoma in which the angiokeratoma itself reduced in size through transepidermal elimination, possibly caused by contact dermatitis.
An Immunohistochemical Study of BCA‐225 in Various Skin Cancers
Authors: Ansai Shinichi , Hozumi Yutaka , Kondo Shigeo 
Published: April 2015

AbstractWe performed an immunohistochemical study of BCA‐225, which is a glycoprotein secreted by the T47D breast carcinoma cell line and recognized by monoclonal antibody BRST‐1 (clone name: CU‐18), in normal skin and various skin cancers. In normal skin, BCA‐225 was positive only in the secretory portion of both eccrine and apocrine glands and in mature cells of the sebaceous gland. We observed 10 cases of squamous cell carcinoma of the skin, 10 cases of basal cell carcinoma without sebaceous differentiation, 3 cases of basal cell carcinoma with sebaceous differentiation, 6 cases of malignant trichilemmoma, 8 cases of eccrine porocarcinoma, 3 cases of ductal carcinoma, 1 case of malignant clear cell hidradenoma, 1 case of apocrine adenocarcinoma, 6 cases of extra‐ocular sebaceous carcinoma, 5 cases of extramammary Paget's disease with underlying adenocarcinoma, and 11 cases of extramammary Paget's disease without underlying adenocarcinoma. Most of the cases of sweat gland carcinoma, basal cell carcinoma with sebaceous differentiation, sebaceous carcinoma, and extramammary Paget's disease were positive for BCA‐225, while none of the cases of squamous cell carcinoma, basal cell carcinoma without sebaceous differentiation, or malignant trichilemoma were positive. Based on these findings, we believe that BCA‐225 is useful in distinguishing tumors with sweat gland and sebaceous differentiation and extramammary Paget's disease from tumors without such differentiation.
Sebaceous Epithelioma
Authors: Watanabe Rikio , Kawai Kazuhiro , Ito Masaaki , Ito Kaoru , Ikeda Kazuto 
Published: April 2015

AbstractAn 83‐year‐old woman had developed an asymptomatic, yellowish, dome‐shaped skin tumor on her scalp which had enlarged for a period of 60 years to 10 times 10 times 10 mm in size. Histopathologically, the tumor consisted of undifferentiated basaloid cells, differentiated sebaceous cells and transitional cells. Although the basaloid cells resembled those of basal cell epithelioma (BCE), the tumor was distinct from BCE in the following points; existence of cystic spaces, no peripheral palisading, no proliferation of connective tissue stroma, and no tendency toward local invasion. Immunohistochemical studies using antikeratin monoclonal antibodies revealed that the tumor contained both the keratin types of BCE and of sebaceous glands. Electron microscopically, the tumor cells contained lipid droplets and keratohyaline granules in their cytoplasm. It is suggested that “sebaceous epithelioma” is a benign skin tumor which may be distinguished cytologically from BCE.
Febrile Ulceronecrotic Mucha‐Habermann's Disease
Authors: Maekawa Yoshihiro , Nakamura Takehiko , Nogami Reiko 
Published: April 2015

AbstractFebrile ulceronecrotic Mucha‐Habermann's disease (FUMH) was first described by Degos in 1966. In the literature, nine cases of FUMH have been reported in both children and adults. We report a 16‐year‐old boy with the febrile ulceronecrotic type. A review of the nine cases in the literature showed acute necrotic lesions, as well as rare complications such as fever, superinfected lesions and viral infection which are not as common in pityriasis lichenoides et varioliformis acuta. There is no definitive treatment, but systemic corticosteroid, methotrexate, antibiotics (tetracycline, erythromycin), aciclovir, and 4,4‐diaminodiphenyl sulfone (DDS) have been frequently used. The most common histologic feature is mononuclear perivascular infiltrates consisting of T lymphocytes. The etiology is not known, but a hypersensitivity reaction, possibly to an infectious agent, is suggested.
Generalized Argyria after Habitual Use of AgNO3
Authors: Lee Seung Min , Lee Seung Hun 
Published: April 2015

AbstractGeneralized argyria, the systemic dissemination and tissue deposition of silver‐containing particles, is characterized by slate gray discoloration of skin, most pronounced in sun exposed areas.A 33‐year‐old woman visited our dermatologic clinic complaining of frequent oral ulceration for 10 years and generalized discoloration of her skin for 5 years. She had had her tongue painted with silver nitrate repeatedly 6 years ago for the treatment of oral ulcers. Physical examination showed slate gray discoloration of her skin, most pronounced on the face and neck. The oral mucosa, tongue, sclera, and conjunctiva also had a slightly blue‐gray discoloration. Biopsy specimens from the oral mucosa and forearm revealed small brown‐black granules scattered in the dermis and basal lamina of eccrine sweat glands, blood vessels, and hair follicles under the light microscope. Tiny black granules were most numerous in the basal laminae of vessels in electron microscopic observation. Energy dispersive X‐ray microanalysis (EDXA) confirmed that many of the granules contained silver.
Microfluorocytometric Detection of Nuclear DNA Damage to Cancer Cells in Squamous Cell Carcinoma after Hyperthermia
Authors: Ishiguro Kazumori , Hatcho Masahiro , Miyoshi Norio , Fukuda Masaru , Ueda Keiichi 
Published: April 2015

AbstractAn 84‐year‐old woman had a squamous cell carcinoma on her left thigh. Before operation, half of the tumor mass was irradiated with a microwave at 43°C for an hour. After the hyperthermia, the tumor mass was removed, and the DNA instability was investigated by microfluorocytometric measurement of the cells stained with pararosaniline‐Feulgen after hydrolysis with 2N HCl at 30°C. The kinetic parameters k1, which is the rate constant for the generation of an apurinic acid, k2, which is the rate constant for the depolymerization of the apurinic acid and which reflects the degree of DNA instability for acid hydrolysis, and y0, which is the theoretical value of the single‐stranded DNA present initially and reflects the degree of DNA damage, were determined by a computer fitting of the Bateman function to the hydrolysis curve plotting of the fluorescence intensity of the pararosaniline‐Feulgen staining the apurinic acid against the different hydrolysis times. The parameter values y0 and k2 of the cells treated by hyperthermia were much higher than those of the cells treated without hyperthermia. It is our conclusion that this microfluorometric detection would be valuable as an evaluation method for a cancer therapy, although more data from other cancers and treatments will be needed.
Clinical Expression of Secondary Syphilis in a Patient with HIV Infection
Authors: Rodríguez‐Díaz Eloy , Morán‐Estefanía Manuel , López‐Avila Angel , Piris Javier Bravo , Fernández‐Blasco Guadalupe , García Jesús Iglesias , Armijo Miguel 
Published: April 2015

AbstractThere are increasing reports of unusual clinical features and atypical courses of syphilis in patients with acquired immunodeficiency syndrome. Recently, we had the opportunity to study an HIV‐positive female patient with strong manifestations of secondary syphilis. The case is discussed together with the implications of secondary syphilis in her concomitant ocular affliction. Moreover, we comment on the clinico‐therapeutic controversies brought about by the association of infection with Treponema pallidum and HIV.
Sweet's Syndrome with Small Cell Carcinoma of the Lung
Authors: Yamamoto Toshiyuki , Furuse Yoshiro , Nishioka Kiyoshi 
Published: April 2015

AbstractWe report a 59‐year‐old male with Sweet's syndrome who developed small cell carcinoma of the lung one year after the initial diagnosis. To the best of our knowledge, this is the first report of Sweet's syndrome in conjunction with lung cancer. In cases of Sweet's syndrome, a search for not only hematologic disorders but also for solid tumors should be made.
A Case of Bullous Transient Acantholytic Dermatosis
Authors: Yoo Jae Hak , Cho Kwang Hyun , Youn Jai Il 
Published: April 2015

AbstractWe report a case of a bullous variant of transient acantholytic dermatosis in a 59‐year‐old female. Each bullous lesion lasted several weeks and healed without scarring. The lesions were migratory and recurrent without a cleared period. Histopathologic examination revealed an intraepidermal vesicle low in the epidermis. The lesions cleared after 2 months of isotretinoin therapy.
An Adult Case of Histiocytosis X with a Vulvar Ulcer and Multiple Bone Lesions
Authors: Takata Minoru , Taniguchi Akira , Imai Toshio , Hirone Takae , Nonomura Akitaka , Fukui Yonemasa 
Published: April 2015

AbstractA 62‐year‐old female with histiocytosis X presented with a vulvar ulcer. Multiple osteolytic lesions were later detected. Histological examination of the ulcerated skin showed diffuse proliferation of histiocytic cells with folded nuclei and pale eosinophilic cytoplasm. Immunohistochemistry revealed S100 protein and vimentin as well as CD1a, CD4, and HLA‐DR antigens in the proliferating cells. Electron microscopy demonstrated Birbeck granules in the cytoplasm of the cells. The patient was successfully treated by complete surgical excision of the ulcer followed by radiotherapy for recurrent vulvar erythema.
A Case of Linear IgA Bullous Dermatosis with Atypical Clinical and Ultrastructural Manifestations
Authors: Isonokami Masaaki , Hashimoto Koji , Horiguchi Yuji , Yoshikawa Kunihiko 
Published: April 2015

AbstractWe report a case of linear IgA bullous dermatosis which presented with atypical clinical and ultrastructural findings. The patient initially manifested small, subepidermal blisters scattered only in the seborrheic region. On follow‐up, grouped vesicles developed in a circular form similar to dermatitis herpetiformis. They subsided after treatment with diaminodiaphenyl sulphon. Immunofluorescent study showed predominant linear IgA deposits accompanied with IgG and C3 along the basement membrane zone. These deposits existed at the floor of the blister. Ultrastructurally, immunoreactants were demonstrated beneath the lamina densa while separation occurred at lamina lucida.
Action Spectrum for Bergamot‐Oil Phototoxicity Measured by Sunburn Cell Counting
Authors: Yasui Yuhko , Hirone Takae 
Published: April 2015

AbstractThe present study investigated the phototoxic effect of bergamot oil and its photosensitive component, bergapten, on sunburn cell (SBC) production in guinea pig skin. The back skin was pretreated with bergamot oil or bergapten and exposed to monochromatic light under various conditions. After irradiation, skin specimens were excised, and histological sections were prepared. The number of sunburn cells in the interfollicular epidermis was counted. The SBC formation by bergamot oil or bergapten plus UVB radiation was the same as that without pretreatment with any photosensitizer. In contrast, a significant number of SBCs were induced by bergamot oil or bergapten plus UVA radiation, but no SBCs were found after the treatment with UVA alone. The result indicates that bergamot oil or bergapten was photosensitized by UVA irradiation. The SBCs were linearly increased in a UV‐dose dependent manner. On the basis of the regression lines, an action spectrum and spectral peak for the photosensitizers plus UVA were obtained. The action spectrum for bergamot oil‐ and bergapten‐induced SBC formation was in the ranges of 325–365 nm and 325–350 nm, and their spectral peaks were at 335–345 nm and 335–350 nm, respectively. The data are in good accordance with those estimated from skin erythema reactions. Therefore, counting SBCs is a very useful parameter for quantitative evaluation of phototoxicity.