bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strong Saharan Dust Deposition Events Alter Microbial Diversity and Composition in Sediments of High-Mountain Lakes of Sierra Nevada (Spain)
Authors: Castellano-Hinojosa Antonio , Tortosa Germán , Fernández-Zambrano Alejandra , Correa-Galeote David , Bedmar Eulogio J. , Medina-Sánchez Juan M. 
Published: July 2024

AbstractMediterranean high-mountain lakes are being increasingly affected by strong Saharan dust deposition events. However, the ecological impacts of these severe atmospheric episodes remain largely unknown. We examined the effects of a strong Saharan dust intrusion to the Iberian Peninsula in 2022 on the physicochemical parameters and prokaryotic communities in sediments of nine high-mountain lakes of Sierra Nevada (Spain) located above 2800 m.a.s.l and in different orientations (north vs. south). A previous year (2021), with lower Saharan dust deposition with respect to 2022, was used for interannual comparisons. The strong dust deposition to the high-mountain lakes resulted in a significant increase in sediment nutrient availability which was linked to changes in the composition of prokaryotic communities. Decreases in alpha diversity and changes in beta diversity of prokaryotic communities were mainly observed in lakes located in the south compared to the north orientation likely because the former was more affected by the atmospheric dust deposition episode. Dust intrusion to the high-mountain lakes resulted in significant changes in the relative abundance of specific genera involved in important nutrient cycling processes such as phosphate solubilization, nitrogen fixation, nitrification, and denitrification. Saharan dust deposition also increased predicted microbial functionality in all lakes. Our findings show that severe atmospheric dust inputs to remote high-mountain lakes of Sierra Nevada can have significant biogeochemical and biodiversity consequences through changes in nutrient availability and prokaryotic communities in sediments of these freshwater ecosystems. This information contributes to understanding how Mediterranean high-mountain lakes of Sierra Nevada face strong intrusions of Saharan dust and their ecological consequences.
A novel Saclayvirus Acinetobacter baumannii phage genomic analysis and effectiveness in preventing pneumonia
Authors: Li Shibin , Wei Bingdong , Xu Le , Cong Cong , Murtaza Bilal , Wang Lili , Li Xiaoyu , Li Jibin , Xu Mu , Yin Jiajun , Xu Yongping 
Published: July 2024

Abstract
Acinetobacter baumannii, which is resistant to multiple drugs, is an opportunistic pathogen responsible for severe nosocomial infections. With no antibiotics available, phages have obtained clinical attention. However, since immunocompromised patients are often susceptible to infection, the appropriate timing of administration is particularly important. During this research, we obtained a lytic phage vB_AbaM_P1 that specifically targets A. baumannii. We then assessed its potential as a prophylactic treatment for lung infections caused by clinical strains. The virus experiences a period of inactivity lasting 30 min and produces approximately 788 particles during an outbreak. Transmission electron microscopy shows that vB_AbaM_P1 was similar to the Saclayvirus. Based on the analysis of high-throughput sequencing and bioinformatics, vB_AbaM_P1 consists of 107537 bases with a G + C content of 37.68%. It contains a total of 177 open reading frames and 14 tRNAs. No antibiotic genes were detected. In vivo experiments, using a cyclophosphamide-induced neutrophil deficiency model, tested the protective effect of phage on neutrophil-deficient rats by prophylactic application of phage. The use of phages resulted in a decrease in rat mortality caused by A. baumannii and a reduction in the bacterial burden in the lungs. Histologic examination of lung tissue revealed a decrease in the presence of immune cells. The presence of phage vB_AbaM_P1 had a notable impact on preventing A. baumannii infection, as evidenced by the decrease in oxidative stress in lung tissue and cytokine levels in serum. Our research offers more robust evidence for the early utilization of bacteriophages to mitigate A. baumannii infection.

Key points
•A novel Saclayvirus phage infecting A. baumannii was isolated from sewage.
•The whole genome was determined, analyzed, and compared to other phages.
•Assaying the effect of phage in preventing infection in neutrophil-deficient models.
The true history of the Hueter-Volkmann law
Authors: Bartoníček Jan , Naňka Ondřej 
Published: July 2024

Abstract
Introduction
The Hueter-Volkmann law (HVL) of the response of growth plate to compression load is a basic concept in orthopaedics. However, little is known about the origin of HVL and its history.

Materials and methods
A literature search was performed in original publications and historical sources.

Results
An analysis of all Volkmann´s and Hueter´s texts has shown that none of their publications was based on experiments, but on the data in the literature and their own clinical observations. They did not deal at all with the effect of pressure on the growth plate and mentioned this structure only marginally. The authors coined the opinion that increased pressure retards and decreased pressure accelerates bone growth. Julius Wolff criticized the HVL and concentrated all his arguments in the book “The law of bone remodeling”. According to him, increased pressure leads to bone formation, decreased pressure to its resorption. The Wolff-Volkmann dispute was addressed in the German literature by a number of authors. Walther Müller in his monograph “The normal and pathological physiology of the bone” criticized Wolff for his concept of interstitial bone growth. In Müller´s view, HVL applies to the growing bone and Wolff confuses growth with hypertrophy of the mature bone.

Conclusion
The circumstances of the emergence of HVL are inaccurately and incompletely described in the current literature, as they are mostly taken from secondary sources. HVL, as it is presented today, is not the original formulation, but the result of a long historical evolution.
Performance of an Anti-Phase Technology-Powered Microwave Ablation System on Ex Vivo Liver, Lung and Kidney: Analysis of Temperature Trend, Ablation Size and Sphericity
Authors: Namakshenas Pouya , Arcaini Tommaso , Cesare Benedetta , Dorato Alessandro , Durante Elena , Ricci Milena , Santucci Domiziana , Saccomandi Paola , Faiella Eliodoro 
Published: July 2024

Abstract

Purpose
Investigating the performance of the new Dophi™ M150E Microwave Ablation System, in terms of temperature distribution, ablation size and shape, reproducibility.

Materials and Methods
The Dophi™ M150E Microwave Ablation System was tested on ex vivo liver, lung and kidney, at 6 different settings of time, power and number of MW antennas (single antenna: 50 and 100 W at 5 and 10 min; double antenna: 75 W at 5 and 10 min). The temperature distribution was recorded by Fiber Bragg Grating sensors, placed at different distances from the antennas. The ablation axes were measured and the sphericity index was calculated.

Results
The standard deviation of ablation axes was < 5 mm, except at the highest energy and time setting for the lung. A maximum temperature rise of ~ 80 °C was measured. The measured ablation axes are overall comparable with the manufacture’s values, especially at lower power and with one MW antenna (average maximum difference is 7 mm). The mean sphericity index of 0.95, 0.79 and 0.9 was obtained for the liver, lung and kidney, respectively, with a single antenna. With double antenna setup, the sphericity index was closer to 1 when 75 W for 10 min were used.

Conclusions
Dophi™ M150E allows good reproducibility of ablation axes for all cases except in the lung at the highest energy level. With one antenna, an almost spherical ablation area for the liver and kidney was obtained. Using double antenna results in more homogeneous temperature distribution within the tissue compared to single antenna.

Graphical Abstract
Use of 3D foot and ankle puzzle enhances student understanding of the skeletal anatomy in the early years of medical school
Authors: Al-Ani Sami A. , Chandla Danny , Delieu John , Yu Sinling Tiffany , Fratini Antonio , Gkountiou Renia , Stocker Claire J. 
Published: July 2024

Abstract
Purpose
3D visualization is an important part of learning anatomy with cadavers generally used to effectuate this. However, high cost, ethical considerations, and limited accessibility can often limit the suitability of cadavers as teaching tools. Anatomical 3D printed models offer an alternative tool for teaching gross anatomy due to their low cost and accessibility. This study aims to investigate if combing gamification with 3D printed models can enhance the learning experience and be effective for teaching anatomy.

Methods
3D printed models of the bones of the foot and ankle were generated, and 267 first-year medical students from 2 consecutive cohorts worked in groups to put it together as a puzzle. Participants completed a questionnaire regarding perceptions of 3D models and their knowledge of foot anatomy, before and after the session and were asked to provide comments.

Results
Analysis of the responses showed a significant increase in the confidence of the learners in their anatomy knowledge and an increased appreciation of the role that 3D models have in enhancing the learning experience. After the session, there were many comments saying how enjoyable and engaging 3D models were.

Conclusion
Through the puzzle element of the session, the students were challenged mentally to work out the anatomical features of the foot and ankle. The combined elements of the puzzle and the features of the 3D model assembly made the activity fun and conducive to active learning. The possibility of having fun was not something the students had considered before the session.
Early-life risk factors which govern pro-allergic immunity
Authors: Ptaschinski Catherine , Gibbs Bernhard F. 
Published: July 2024

AbstractAllergic diseases affect up to 40% of the global population with a substantial rise in food allergies, in particular, over the past decades. For the majority of individuals with allergy fundamental programming of a pro-allergic immune system largely occurs in early childhood where it is crucially governed by prenatal genetic and environmental factors, including their interactions. These factors include several genetic aberrations, such as filaggrin loss-of-function mutations, early exposure to respiratory syncytial virus, and various chemicals such as plasticizers, as well as the influence of the gut microbiome and numerous lifestyle circumstances. The effects of such a wide range of factors on allergic responses to an array of potential allergens is complex and the severity of these responses in a clinical setting are subsequently not easy to predict at the present time. However, some parameters which condition a pro-allergic immune response, including severe anaphylaxis, are becoming clearer. This review summarises what we currently know, and don’t know, about the factors which influence developing pro-allergic immunity particularly during the early-life perinatal period.
Free-breathing 3D phase-resolved functional lung MRI vs breath-hold hyperpolarized 129Xe ventilation MRI in patients with chronic obstructive pulmonary disease and healthy volunteers
Authors: Klimeš Filip , Kern Agilo Luitger , Voskrebenzev Andreas , Gutberlet Marcel , Grimm Robert , Müller Robin Aaron , Behrendt Lea , Kaireit Till Frederik , Glandorf Julian , Alsady Tawfik Moher , Wacker Frank , Hohlfeld Jens M. , Vogel-Claussen Jens 
Published: July 2024

Abstract

Objectives
3D phase-resolved functional lung (PREFUL) MRI offers evaluation of pulmonary ventilation without inhalation of contrast agent. This study seeks to compare ventilation maps obtained from 3D PREFUL MRI with a direct ventilation measurement derived from 129Xe MRI in both patients with chronic obstructive pulmonary disease (COPD) and healthy volunteers.

Methods
Thirty-one patients with COPD and 12 healthy controls underwent free-breathing 3D PREFUL MRI and breath-hold 129Xe MRI at 1.5 T. For both MRI techniques, ventilation defect (VD) maps were determined and respective ventilation defect percentage (VDP) values were computed. All parameters of both techniques were compared by Spearman correlation coefficient (r) and the differences between VDP values were quantified by Bland–Altman analysis and tested for significance using Wilcoxon signed-rank test. In a regional comparison of VD maps, spatial overlap and Sørensen–Dice coefficients of healthy and defect areas were computed.

Results
On a global level, all 3D PREFUL VDP values correlated significantly to VDP measure derived by 129Xe ventilation imaging (all r > 0.65; all p < 0.0001). 129Xe VDP was significantly greater than 3D PREFUL derived VDPRVent (mean bias = 10.5%, p < 0.001) and VDPFVL-CM (mean bias = 11.3%, p < 0.0001) but not for VDPCombined (mean bias = 1.7%, p = 0.70). The total regional agreement of 129Xe and 3D PREFUL VD maps ranged between 60% and 63%.

Conclusions
Free-breathing 3D PREFUL MRI showed a strong correlation with breath-hold hyperpolarized 129Xe MRI regarding the VDP values and modest differences in the detection of VDs on a regional level.

Clinical relevance statement
3D PREFUL MRI correlated with 129Xe MRI, unveiling regional differences in COPD defect identification. This proposes 3D PREFUL MRI as a ventilation mapping surrogate, eliminating the need for extra hardware or inhaled gases.

Key Points

Current non-invasive evaluation techniques for lung diseases have drawbacks;

129

Xe MRI is limited by cost and availability.

3D PREFUL MRI correlated with

129

Xe MRI, with regional differences in identifying COPD defects.

3D PREFUL MRI can provide ventilation mapping without the need for additional hardware or inhaled gases.
Grading urothelial carcinoma with probe-based confocal laser endomicroscopy during flexible cystoscopy
Authors: de Ruiter Ben-Max , Freund Jan Erik , Savci-Heijnink C. Dilara , van Hattum Jons W. , de Reijke Theo M. , Baard Joyce , Kamphuis Guido M. , de Bruin D. Martijn , Oddens Jorg R. 
Published: July 2024

Abstract
Purpose
Urothelial bladder cancer (UCB) care requires frequent follow-up cystoscopy and surgery. Confocal laser endomicroscopy (CLE) is a probe-based optical technique that can provide real-time microscopic evaluation with the potential for outpatient grading of UCB. This study aims to investigate the diagnostic accuracy and interobserver variability for the grading of UCB with CLE during flexible cystoscopy (fCLE).

Methods
Participants scheduled for transurethral resection of papillary bladder tumors were prospectively included for intra-operative fCLE. Exclusion criteria were flat lesions, fluorescein allergy or pregnancy. Two independent observers evaluated fCLE, classifying tumors as low- or high-grade urothelial carcinoma (LGUC/HGUC) or benign. Interobserver agreement was calculated with Cohens kappa (κ) and diagnostic accuracy with 2 × 2 tables. Histopathology was the reference test.

Results
Histopathology of 34 lesions revealed 14 HGUC, 14 LGUC and 6 benign tumors. Diagnostic yield for fCLE was 80–85% with a κ of 0.75. Respectively, sensitivity, specificity, NPV and PPV were: for benign tumors 0–20%, 96–100%, unmeasureable-50% and 87%, for LGUC 57–64%, 41–58%, 44–53% and 54–69% and for HGUC 38–57%, 56–68%, 38–57% and 56–68%, with an interobserver agreement of κ 0.61.

Conclusion
fCLE is currently insufficient to grade UCB.
A fixed-stress splitting method for nonlinear poroelasticity
Authors: Kraus Johannes , Kumar Kundan , Lymbery Maria , Radu Florin A. 
Published: July 2024

AbstractIn this paper we consider a nonlinear poroelasticity model that describes the quasi-static mechanical behaviour of a fluid-saturated porous medium whose permeability depends on the divergence of the displacement. Such nonlinear models are typically used to study biological structures like tissues, organs, cartilage and bones, which are known for a nonlinear dependence of their permeability/hydraulic conductivity on solid dilatation. We formulate (extend to the present situation) one of the most popular splitting schemes, namely the fixed-stress split method for the iterative solution of the coupled problem. The method is proven to converge linearly for sufficiently small time steps under standard assumptions. The error contraction factor then is strictly less than one, independent of the Lamé parameters, Biot and storage coefficients if the hydraulic conductivity is a strictly positive and Lipschitz-continuous function.
Association between carotid plaque progression and persistent endothelial dysfunction in an infarct-related coronary artery in STEMI survivors
Authors: Horikoshi Takeo , Nakamura Takamitsu , Yamada Ryota , Yoshizaki Toru , Watanabe Yosuke , Uematsu Manabu , Kobayashi Tsuyoshi , Sato Akira 
Published: July 2024

AbstractPersistent coronary endothelial dysfunction predicts future adverse events; however, performing multiple invasive endothelial function tests is difficult in actual clinical practice. This study examined the association between carotid plaque progression and persistent coronary endothelial dysfunction using serial assessments of the coronary vasomotor response to acetylcholine (ACh) in the infarct-related artery (IRA) among patients with ST-elevation acute myocardial infarction (STEMI). This study included 169 consecutive patients with a first STEMI due to the left anterior descending coronary artery (LAD) occlusion who underwent successful percutaneous coronary intervention. The vasomotor response to ACh in the LAD was measured within two weeks after acute myocardial infarction (AMI) (first test) and repeated at six months (second test) after AMI. Ultrasonography of the bilateral common carotid artery and internal carotid artery was performed during the acute phase, and the thickest intima-media thickness (IMT) of either artery was measured as the maximum IMT. After six months, the IMT at the site of maximal IMT was re-measured to determine the carotid plaque progression. Finally, 87 STEMI patients analyzed. At 6 months, 25 patients (28.7%) showed carotid plaque progression. In a multivariable analysis, carotid plaque progression was identified as an independent predictor of persistent coronary endothelial dysfunction, both in terms of coronary diameter response [odd ratio (OR) 3.22, 95% confidence interval (95% CI) 1.13–9.15, p = 0.03] and coronary flow response [OR 2.65, 95% CI 1.01–7.00, p = 0.04]. Independently, carotid plaque progression is linked to persistent endothelial dysfunction in the IRA among STEMI survivors.
Elevation dependent change in ERA5 precipitation and its extremes
Authors: Ferguglia Olivia , Palazzi Elisa , Arnone Enrico 
Published: July 2024

AbstractMountain regions are recognised as hot-spots of climate change. Although the existence of an Elevation-Dependent Warming has been extensively confirmed in several mountain areas of the globe, fewer studies have analysed the elevational stratification of temporal trends of other climate variables, and particularly for precipitation. This study analyses changes in mean precipitation and its extremes in ERA5 global reanalysis data in key mountain areas of the globe, along with their elevational dependence, from 1951 to 2020. These include the Tibetan Plateau, the US Rocky Mountains, the Greater Alpine Region, and the Andes, as representative of different latitudes and climatic influences. Our analysis reveals common patterns of elevational dependent change in precipitation and its extremes in most of the mountainous areas, which emerge beyond their geographical differences. A positive elevational gradient of trends of extreme precipitation indices is found in the Tibetan Plateau, the Greater Alpine Region, and the subtropical Andes, highlighting a wetting effect (positive trends) at very high elevations. In contrast, the Rocky Mountains exhibit a negative elevational gradient, with a drying effect (negative trends) increasing with the elevation. Notably, a simple linear regression proved to be effective to describe the stratification of change in the Greater Alpine Region and the Rocky Mountains, whereas more complex vertical patterns need to be considered for the Andes and the Tibetan Plateau. Mean precipitation, heavy ($$\ge$$
≥
10 mm) precipitation and the length of consecutive wet days show a consistent elevation-dependent stratification within each of the study areas, suggesting possible common driving mechanisms.
Experiences of patients with endometriosis with a digital health application: a qualitative analysis
Authors: Zugaj Marco Richard , Germeyer Ariane , Kranz Karina , Züger Andrea , Keßler Jens 
Published: July 2024

Abstract
Background
Endometriosis is a frequent disease in women of reproductive age in which the endometrium occurs outside the uterine cavity. Multimodal treatment approaches are necessary due to loss of quality of life and the chronic nature of the disease. Digital health applications (DiGa) are becoming increasingly important. This research project investigates how a healthcare app can influence the subjective experience of illness in patients with endometriosis.

Methods
Empiric data were collected through semi-structured interviews. Data analysis was carried out using qualitative focussed interview analysis. Reliability was ensured by joint interdisciplinary and interprofessional evaluation of the interviews by experts and those affected.

Results
Ten patients with endometriosis and the prescribed healthcare app Endo-App© were examined. Categories were defined from the superordinate categories “Factors influencing the experience of illness” and “Evaluation of the app”. The app provided reliable information, promoted self-efficacy through exercises and strengthened the perception of the individuality of the illness. It helped to minimise nocebo effects from internet research and enabled a positive change of perspective. Patients criticised the time required for data input and had data protection concerns. The educational elements were often seen as redundant. Some patients only used the app briefly, or not at all.

Conclusion
Once a DiGa has been prescribed, it may be useful to explain its use on an outpatient basis and validate regular use. Blind re-prescribing of DiGas should be avoided. Younger patients with a recent diagnosis or patients following rehabilitation may benefit more from prescribing.
The impact of tinnitus on speech perception in noise: a systematic review and meta-analysis
Authors: Madhukesh Sanjana , Palaniswamy Hari Prakash , Ganapathy Kanaka , Rajashekhar Bellur , Nisha Kavassery Venkateswaran 
Published: July 2024

Abstract
Purpose
Tinnitus is a condition that causes people to hear sounds without an external source. One significant issue arising from this condition is the difficulty in communicating, especially in the presence of noisy backgrounds. The process of understanding speech in challenging situations requires both cognitive and auditory abilities. Since tinnitus presents unique challenges, it is important to investigate how it affects speech perception in noise.

Method
In this review, 32 articles were investigated to determine the effect of tinnitus on the effect of speech in noise perception performance. Based on the meta-analysis performed using a random-effects model, meta-regression was used to explore the moderating effects of age and hearing acuity.

Results
A total of 32 studies were reviewed, and the results of the meta-analysis revealed that tinnitus significantly impacts speech in terms of noise perception performance. Additionally, the regression analysis revealed that age and hearing acuity are not significant predictors of speech in noise perception.

Conclusion
Our findings suggest that tinnitus affects speech perception in noisy environments due to cognitive impairments and central auditory processing deficits. Hearing loss and aging also contribute to reduced speech in noise performance. Interventions and further research are necessary to address individual challenges associated with continuous subjective tinnitus.