bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative Opioid Requirements following Roux-en-Y Gastric Bypass in Patients Receiving Continuous Bupivacaine through a Pump System: A Retrospective Review
Authors: Cohen Andrew R. , Smith April N. , Henriksen Brian S. 
Published: May 2013

Background Patients who undergo Roux-en-Y gastric bypass (RYGB) surgery have self-reported considerable postoperative pain, often requiring opioid administration. Objective To determine whether continuous delivery of local anesthetic via an infusion pump system decreased postoperative opioid usage in post-RYGB patients. Methods The electronic health record was used to identify and review 289 patients who underwent RYGB at our institution from January 2009 to October 2011. The treatment group received a continuous infusion of 0.375% bupivacaine administered by intraperitoneal soaker catheter for 48 hours via an infusion pump; the control group did not receive a pump or local anesthetic. Both groups received general anesthesia, nausea prophylaxis, and pain medication. Pain management consisted of opioid-containing patient-controlled analgesia (PCA) for the first 24 hours. Patients transitioned to supplemental intravenous opioid boluses, plus an oral opioid, for the remainder of their stay. Opioid use was measured in terms of morphine equivalents. Secondary outcomes included visual analog scale (VAS) pain scores and length of hospitalization. Results Morphine equivalents over the postoperative time period studied were significantly lower in the bupivacaine group than the control group (133 vs 106 mg, respectively; P = .001). There was no significant difference in VAS scores between the 2 groups ( P = .80). Finally, the length of hospitalization between the 2 groups did not differ ( P = .77). Conclusions We have shown that continuous infusion of bupivacaine, administered via a pain pump system, may have decreased postoperative opioid utilization. There were no differences in VAS scores or length of hospitalization between groups.
Critical ∂̅ problems in one complex dimension and some remarks on conformally invariant variational problems in two real dimensions
Authors: Sharp Ben 
Published: May 2013

Abstract.
We will study a linear first order system, a connection ∂̅ problem, on a vector bundle equipped with a connection, over a Riemann surface. We show optimal conditions on the connection forms which allow one to find a holomorphic frame, or in other words to prove the optimal regularity of our solution. The underlying geometric principle, discovered by Koszul–Malgrange, is classical and well known; it gives necessary and sufficient conditions for a connection to induce a holomorphic structure on a vector bundle over a complex manifold. Here we explore the limits of this statement when the connection is not smooth and our findings lead to a very short proof of the regularity of harmonic maps in two dimensions as well as re-proving a recent estimate of Lamm and Lin concerning conformally invariant variational problems in two dimensions.
Die Rückgabe eines Buches des protestantischen Gelehrten Wilhelm Wundt aus dem Besitz des enteigneten jüdischen Apothekers Leopold Scheyer an seine Erben
Authors: Andert Karin , Kohls Niko 
Published: May 2013

Zusammenfassung:
Dieser Beitrag berichtet über die Geschichte eines Buches des jüdischen Apothekers Leopold Scheyer, das, nachdem es im Jahr 2010 aus dem Bibliotheksbestand der Freien Universität Berlin ausgesondert wurde, antiquarisch erworben, sich als NS-Raubgut herausstellte und jetzt an die Erben zurückerstattet wurde. Ein sich daran anschließendes Studium der Entschädigungsakten gibt Einblick in die Praxis der Wiedergutmachung der Bundesrepublik Deutschland in den Nachkriegsjahren.
Cerebrospinal fluid analyses for the diagnosis of subarachnoid haemorrhage and experience from a Swedish study. What method is preferable when diagnosing a subarachnoid haemorrhage?
Authors: Nagy Karin , Skagervik Ina , Tumani Hayrettin , Petzold Axel , Wick Manfred , Kühn Hans-Jürgen , Uhr Manfred , Regeniter Axel , Brettschneider Johannes , Otto Markus , Kraus Jörg , Deisenhammer Florian , Lautner Ronald , Blennow Kaj , Shaw Leslie , Zetterberg Henrik , Mattsson Niklas 
Published: May 2013

Abstract
Subarachnoid haemorrhage (SAH) has a high mortality and morbidity rate. Early SAH diagnosis allows the early treatment of a ruptured cerebral aneurysm, which improves the prognosis. Diagnostic cerebrospinal fluid (CSF) analyses may be performed after a negative computed tomography scan, but the precise analytical methods to be used have been debated. Here, we summarize the scientific evidence for different CSF methods for SAH diagnosis and describe their implementation in different countries. The principle literature search was conducted using PubMed and Scopus with the search items “cerebrospinal fluid”, “subarachnoid haemorrhage”, and “diagnosis”. CSF analyses for SAH include visual examination, red blood cell counts, spectrophotometry for oxyhaemoglobin or bilirubin determination, CSF cytology, and ferritin measurement. The methods vary in availability and performance. There is a consensus that spectrophotometry has the highest diagnostic performance, but both oxyhaemoglobin and bilirubin determinations are susceptible to important confounding factors. Visual inspection of CSF for xanthochromia is still frequently used for diagnosis of SAH, but it is advised against because spectrophotometry has a superior diagnostic accuracy. A positive finding of CSF bilirubin is a strong indicator of an intracranial bleeding, whereas a positive finding of CSF oxyhaemoglobin may indicate an intracranial bleeding or a traumatic tap. Where spectrophotometry is not available, the combination of CSF cytology for erythrophages or siderophages and ferritin is a promising alternative.
Staging chronic kidney disease and estimating glomerular filtration rate: an opinion paper about the new international recommendations
Authors: Delanaye Pierre , Cavalier Etienne 
Published: May 2013

Abstract
In January 2013, the international recommendations of the KDIGO (for “Kidney Disease: Improving Global Outcomes”) to define chronic kidney disease (CKD) and classify patients in CKD stages have been published. In this opinion article, we will review and discuss the most important guidelines proposed about CKD staging and glomerular filtration rate (GFR) estimating. In particular, we question the choice of fixed knot values at 60 mL/min/1.73 m² to define CKD. We also question the strategies proposed to measure and use cystatin C results.
Survey of national guidelines, education and training on phlebotomy in 28 European countries: an original report by the European Federation of Clinical Chemistry and Laboratory Medicine (EFLM) working group for the preanalytical phase (WG-PA)
Authors: Simundic Ana-Maria , Cornes Michael , Grankvist Kjell , Lippi Giuseppe , Nybo Mads , Kovalevskaya Svjetlana , Sprongl Ludek , Sumarac Zorica , Church Stephen 
Published: May 2013

Abstract

Background: European questionnaire survey was conducted by the European Federation of Clinical Chemistry and Laboratory Medicine Working Group for the Preanalytical Phase (EFLM WG-PA) to assess how phlebotomy is performed in EFLM countries, including differences in personnel, level of education and skills, and to investigate the presence and compliance of national phlebotomy guidelines on this matter.

Methods: A questionnaire was constructed containing questions elucidating different aspects of the organization behind the phlebotomy praxis on a national basis, including questions on the staff performing phlebotomy, the education of these staff members, and the existence of and adherence to national guidelines. All 39 EFLM member countries were invited to participate.

Results: In total 28/39 (72%) EFLM member countries responded. Seven out of the 28 (25%) have national phlebotomy guidelines and five have implemented other guidelines. The estimated compliance with phlebotomy guidance for the laboratories in the countries that have national guidelines available is poor, regardless to whether the phlebotomy was under the laboratory control or not. Most countries were interested in EFLM guidelines and to participate in a pilot EFLM preanalytical phase external quality assessment (EQA) scheme. In the responding EFLM member countries, the majority of phlebotomy is performed by nurses and laboratory technicians. Their basic education is generally 4–5 years of high school, followed by 2–5 years of colleague or university studies. Only a third (10/28; 36%) of the participating member countries has any specific training available as a continuous educational resource. A specific training for phlebotomy is not part of the education required to become qualified in 6/28 (21%) and 9/28 (32%) of countries for nurses and laboratory technicians, respectively. In countries and professions where training is required, most require more than 5 h of training.

Conclusions: Based on the results of this survey we conclude the following: 1) There is a need to assess the quality of current practices, compliance to the CLSI H3-A6 guidelines and to identify some most critical steps which occur during phlebotomy, in different healthcare settings, across Europe; 2) Existing CLSI H3-A6 phlebotomy guidelines should be adapted and used locally in all European countries which do not have their own guidelines; 3) National EFLM societies need to be engaged in basic training program development and continuous education of healthcare phlebotomy staff (implementing the certification of competence).
Motivic integration in all residue field characteristics for Henselian discretely valued fields of characteristic zero
Authors: Cluckers Raf , Loeser François 
Published: May 2013

Abstract

We extend the formalism and results on motivic integration from [Invent. Math. 173 (2008), 23–121] to mixed characteristic discretely valued Henselian fields with bounded ramification. We also generalize the equicharacteristic zero case of loc. cit. by giving, in all residue characteristics, an axiomatic approach (instead of only using Denef–Pas languages) and by using richer angular component maps. In this setting we prove a general change of variables formula and a general Fubini Theorem. Our set-up can be specialized to previously known versions of motivic integration by e.g. the second author and J. Sebag and to classical p-adic integrals.
A uniqueness theorem for Frobenius manifolds and Gromov–Witten theory for orbifold projective lines
Authors: Ishibashi Yoshihisa , Shiraishi Yuuki , Takahashi Atsushi 
Published: May 2013

Abstract

We prove that the Frobenius structure constructed from the Gromov–Witten theory
for an orbifold projective line with at most three orbifold points is uniquely determined by the
Witten–Dijkgraaf–Verlinde–Verlinde equations with certain natural initial conditions.
Synthesis, antimicrobial and DFT studies of novel fused thiazolopyrimidine derivatives
Authors: Gupta Richa , Chaudhary Ram Pal 
Published: May 2013

Abstract
The reaction of dihydropyrimidine-2(1H)-thione 4, obtained by the condensation of chalcone 3 with thiourea, with chloroacetic acid and 1,2-dibromoethane furnished compounds 5 and 6 and not their respective isomers 8 and 9. The regiochemistry of the cyclized products and their structure was established by elemental analysis, 1H NMR, 13C NMR, IR and mass spectral data. Density functional theory (DFT) calculations have been carried out for compound 5a and its isomer 8a with Jaguar version 6.5112 using B3LYP density functional method and 6–31G** basis set. 1H and 13C NMR spectra of compound 5a and its possible isomer 8a have been calculated and correlated with experimental results. 2-Arylidene derivatives of 5 were obtained by two routes and their structure was established by spectral data. Compounds 4–7 were screened for their antimicrobial activities.
Indoloquinolines as DNA binding ligands
Authors: Riechert-Krause Fanny , Weisz Klaus 
Published: May 2013

Abstract
Indoloquinoline alkaloids isolated from natural sources are known for their broad spectrum of biological activities. The cytotoxic action of some derivatives is thought to arise from their interaction with genomic DNA driven by favorable stacking interactions of the intercalated indoloquinoline skeleton with the DNA base pairs. In addition to double-helical DNA, indoloquinoline derivatives have also been found to bind DNA triplexes as well as G-quadruplexes with high affinity, opening interesting perspectives towards the development of indoloquinoline-based DNA binding ligands with numerous potential applications in therapeutics, diagnostics and microbiology. The present paper presents an overview of studies dealing primarily with the binding of indoloquinoline analogs to double-helical, triple-helical or quadruplex targets. Available thermodynamic and structural data that also include few experimentally determined three-dimensional structures of DNA-drug complexes highlight key elements for an improved binding affinity but also important structural aspects for the design of structure-selective analogs, prerequisite for many potential applications.
Laser-induced fluorescence (LIF) of lignin and lignin model compounds in Raman spectroscopy
Authors: Lähdetie Anni , Nousiainen Paula , Sipilä Jussi , Tamminen Tarja , Jääskeläinen Anna-Stiina 
Published: May 2013

Abstract
Raman spectroscopy is a technique that provides structural information on lignin and other components of wood and pulp in situ. However, especially lignin-containing samples may produce laser-induced fluorescence (LIF) that overlaps with Raman bands. In the worst case, this background signal can overwhelm the weaker Raman signal completely. In this study, the LIF of lignin was investigated with the excitation wavelength 532 nm applied in Raman spectroscopy to clarify the correlations between lignin structure and LIF intensity. Raman spectroscopic analyses with lignin model compounds illustrated that the 5-5′ structures induce LIF. It was also shown that the intensity of LIF was significantly less intense when the 5-5′ model compound was structurally rigid (as in dibenzodioxocin) compared with the flexible simple counterpart. The comparison between the free phenolic model compounds with the methylated analogue showed that the presence of the free phenolic structure was not a prerequisite for LIF. It was thus concluded that the conformation of the molecule is the key factor with respect to fluorescence. The role of conformational aspects was further investigated by comparing wood with chemical pulps and isolated lignins.
Effects of DHEA on metabolic and endocrine functions of adipose tissue
Authors: Karbowska Joanna , Kochan Zdzislaw 
Published: May 2013

Abstract
Dehydroepiandrosterone (DHEA) and its sulfate ester, DHEAS, are the major circulating adrenal steroids and serve as substrates for sex hormone biosynthesis. DHEA is effectively taken up by adipose tissue, where the concentrations of free DHEA are four to ten times higher than those found in the circulation. DHEA reduces adipose tissue mass and inhibits the proliferation and differentiation of adipocytes; it may also protect against obesity by lowering the activity of stearoyl-CoA desaturase 1 in fat cells. Recent studies demonstrate that DHEA stimulates triacylglycerol hydrolysis in adipose tissue by increasing the expression and activity of adipose triglyceride lipase and hormone-sensitive lipase, the key enzymes of lipolysis. DHEA has been shown to modulate insulin signaling pathways, enhance glucose uptake in adipocytes, and increase insulin sensitivity in patients with DHEA deficiency or abnormal glucose tolerance. Additionally, by suppressing the activity of 11β-hydroxysteroid dehydrogenase 1 in adipocytes, DHEA may promote intra-adipose inactivation of cortisol to cortisone. Several studies have demonstrated that DHEA may also regulate the expression and secretion of adipokines such as leptin, adiponectin, and resistin. The effects of DHEA on adipokine expression in adipose tissue are depot-specific, with visceral fat being the most responsive. The mechanisms underlying DHEA actions in adipose tissue are still unclear; however, they involve nuclear receptors such as androgen receptor and peroxisome proliferator-activated receptors γ and α. Because clinical trials investigating the effects of DHEA failed to yield consistent results, further studies are needed to clarify the role of DHEA in the regulation of human adipose tissue physiology.
Role of maternal nutrition in programming adiposity in the offspring: potential implications of glucocorticoids
Authors: Breton Christophe 
Published: May 2013

Abstract
The epidemiological studies initially indicated that maternal undernutrition leading to a low birth weight may predispose to the long-lasting energy balance disorders. A high birth weight due to maternal obesity or diabetes, inappropriate early postnatal nutrition, and rapid catch-up growth, may also sensitize to an increased risk of obesity. As stated by the developmental origin of health and disease concept, the perinatal perturbation of the fetus/neonate nutrient supply might be a crucial determinant of the individual programming of the body weight set point. The adipose tissue is considered as the main fuel storage unit involved in the maintenance of the energy homeostasis. Several models have demonstrated that this tissue is a prime target of the developmental programming in a gender- and depot-specific manner. In the rodents, the perinatal period of life corresponds largely to the period of adipogenesis. In contrast, this phenomenon essentially takes place before birth in bigger mammals. Despite these different developmental time windows, the altricial and precocial species share several common offspring programming mechanisms. Thus, the adipose tissue of the offspring from malnourished dams exhibited impaired glucose uptake and leptin/insulin resistance with increased proinflammatory markers. It also displayed a modified sympathetic activity, circadian rhythm, fatty acid composition, and thermogenesis. This might lead to the reprogrammed metabolism and distribution of the adipose tissue with enhanced adipogenesis and fat accumulation predisposing to adiposity. The inappropriate glucocorticoid (GC) levels and modified tissue sensitivity might be key actors of perinatal programming and long-lasting altered adipose tissue activity in the offspring. Following maternal malnutrition, the epigenetic mechanisms might also be responsible for the adipose tissue programming.
Overexpression of miR-126 promotes the differentiation of mesenchymal stem cells toward endothelial cells via activation of PI3K/Akt and MAPK/ERK pathways and release of paracrine factors
Authors: Huang Feng , Fang Zhen-fei , Hu Xin-qun , Tang Liang , Zhou Sheng-hua , Huang Jian-ping 
Published: May 2013

Abstract
The endothelial cell (EC)-specific miRNA, miR-126, is known to promote angiogenesis in response to angiogenic factors by repressing negative regulators of signal transduction pathways; however, whether miR-126 might regulate the differentiation of stem cells toward endothelial lineage remains unknown. To answer this question, in this study mesenchymal stem cells (MSCs) harvested from C57BL/6 mouse bone marrow were transfected with miR-126 (MSCmiR-126) using recombinant lentiviral vectors. Results showed the para-secretion and the expression levels of phosphorylated PI3K p85, Akt, p38, ERK1 protein in the MSCmiR-126 group were dramatically increased when compared with the control group. With half culture medium refreshed every 3 days, a small number of 6-day-cultured MSCmiR-126 differentiated into endothelial-like cells and most of 9-day-cultured MSCmiR-126 formed a cobblestone-like structure. These differentiated cells evidently expressed EC-specific makers and possessed mature ECs function, while inhibition of paracrine factors suppressed the MSC-EC differentiation. Strikingly, the increased secretion of MSCmiR-126 and their endothelial-differentiated potential were deprived by using a PI3K or MEK chemical inhibitor. Our results suggest that overexpression of miR-126 agumenting the endothelial differentiation of MSCs might in part be attributable to the activation of PI3K/Akt and MAPK/ERK pathways and an increased release of paracrine factors.
Subcellular localization and RNP formation of IGF2BPs (IGF2 mRNA-binding proteins) is modulated by distinct RNA-binding domains
Authors: Wächter Kristin , Köhn Marcel , Stöhr Nadine , Hüttelmaier Stefan 
Published: May 2013

Abstract
The IGF2 mRNA-binding protein family (IGF2BPs) directs the cytoplasmic fate of various target mRNAs and controls essential cellular functions. The three IGF2BP paralogues expressed in mammals comprise two RNA-recognition motifs (RRM) as well as four KH domains. How these domains direct IGF2BP paralogue-dependent protein function remains largely elusive. In this study, we analyze the role of KH domains in IGF2BPs by the mutational GXXG-GEEG conversion of single KH domain loops in the context of full-length polypeptides. These analyses reveal that all four KH domains of IGF2BP1 and IGF2BP2 are essentially involved in RNA-binding in vitro and the cellular association with RNA-binding proteins (RBPs). Moreover the KH domains prevent the nuclear accumulation of these two paralogues and facilitate their recruitment to stress granules. The role of KH domains appears less pronounced in IGF2BP3, because GxxG-GEEG conversion in all four KH domains only modestly affects RNA-binding, subcellular localization and RNA-dependent protein association of this paralogue. These findings indicate paralogue-dependent RNA-binding properties of IGF2BPs which likely direct distinct cellular functions. Our findings suggest that IGF2BPs contact target RNAs via all four KH domains. This implies significant structural constraints, which presumably allow the formation of exceedingly stable protein-RNA complexes.
Structure and allosteric regulation of eukaryotic 6-phosphofructokinases
Authors: Schöneberg Torsten , Kloos Marco , Brüser Antje , Kirchberger Jürgen , Sträter Norbert 
Published: May 2013

Abstract
Although the crystal structures of prokaryotic 6-phosphofructokinase, a key enzyme of glycolysis, have been available for almost 25 years now, structural information about the more complex and highly regulated eukaryotic enzymes is still lacking until now. This review provides an overview of the current knowledge of eukaryotic 6-phosphofructokinase based on recent crystal structures, kinetic analyses and site-directed mutagenesis data with special focus on the molecular architecture and the structural basis of allosteric regulation.
Synthesis of a novel benzodifuran derivative and its molecular recognition of poly rA RNA
Authors: Roviello Giovanni N. , Roviello Valentina , Musumeci Domenica , Pedone Carlo 
Published: May 2013

Abstract
In this manuscript, we describe a synthetic approach to a novel benzodifuran derivative as well as CD studies regarding its ability to interact with DNA and RNA. After the chemical synthesis and ESI-MS and NMR characterization, this heteroaromatic molecule was investigated by CD spectroscopy in order to evaluate it as a potential nucleic acid binder. Interestingly, the benzodifuran compound was found to be able to induce conformational changes in both DNA and RNA homoadenine molecules forming in the latter case a complex with a 6:1 benzodifuran/nucleobase stoichiometric ratio, as evidenced by CD titration experiments.