bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frameworks describing the transition to open labour employment for persons with mild and moderate intellectual disability: a scoping review protocol
Authors: du Toit Taryn Lynne , Soeker Mogammad Shaheed , Wegner Lisa 
Published: July 2024

IntroductionPersons with mild and moderate intellectual disability (MMID) have the right to participate in all aspects of society and be afforded equal opportunities to realise their full potential. However, it is a challenge for persons with MMID to find and maintain employment due to many different barriers. Currently, there is no contextually relevant framework in South Africa that considers the unique employment support needs and accommodation of persons with MMID, which can guide and inform the transition to employment interventions, strategies and national policy directives.Methods and analysisThe proposed scoping review will be guided by the methodological framework developed by a working group from the Joanna Briggs Institute (JBI) and the JBI Collaboration. The search for relevant studies will be conducted across 12 research databases. Reference mining of review studies will be conducted. A three-step search strategy will be used, including the use of information management software to manage the search results. Three independent reviewers will screen the full-text studies to finalise the list of included and excluded studies. Included studies will be assessed for methodological quality using a critical appraisal tool. Data will be charted by one independent reviewer and assessed by two independent reviewers, using a data charting instrument. The data chart will be presented using tables and charts describing the demographic features of the reviewed studies, with an explanation of the thematically analysed results.Ethics and disseminationThis scoping review has received ethical approval (BM22/10/11). A summary of the findings of the study will be published in a peer-reviewed journal. This scoping review will contribute to a better understanding of the key characteristics or factors underpinning the components of a multilevel transition to employment framework that will result in open labour market employment for persons with MMID.
Preventing adverse events during paediatric cancer treatment: protocol for a multi-site hybrid randomised controlled trial of catheter lock solutions (the CLOCK trial)
Authors: Ullman Amanda , Takashima Mari , Gibson Victoria , Comber Elouise , Borello Eloise , Bradford Natalie , Byrnes Joshua , Cole Roni , Eisenstat David , Henson Nicole , Howard Philippa , Irwin Adam , Keogh Samantha , Kleidon Tricia , Martin Michelle , McCleary Karen , McLean Jordana , Moloney Susan , Monagle Paul , Moore Andrew , Newall Fiona , Noyes Michelle , Rowan Gemma , St John Amanda , Wood Andrew , Wolf Joshua , Ware Robert 
Published: July 2024

IntroductionCentral venous access devices (CVADs) are commonly used for the treatment of paediatric cancer patients. Catheter locking is a routine intervention that prevents CVAD-associated adverse events, such as infection, occlusion and thrombosis. While laboratory and clinical data are promising, tetra-EDTA (T-EDTA) has yet to be rigorously evaluated or introduced in cancer care as a catheter lock.Methods and analysisThis is a protocol for a two-arm, superiority type 1 hybrid effectiveness-implementation randomised controlled trial conducted at seven hospitals across Australia and New Zealand. Randomisation will be in a 3:2 ratio between the saline (heparinised saline and normal saline) and T-EDTA groups, with randomly varied blocks of size 10 or 20 and stratification by (1) healthcare facility; (2) CVAD type and (3) duration of dwell since insertion. Within the saline group, there will be a random allocation between normal and heparin saline. Participants can be re-recruited and randomised on insertion of a new CVAD. Primary outcome for effectiveness will be a composite of CVAD-associated bloodstream infections (CABSI), CVAD-associated thrombosis or CVAD occlusion during CVAD dwell or at removal. Secondary outcomes will include CABSI, CVAD-associated-thrombosis, CVAD failure, incidental asymptomatic CVAD-associated-thrombosis, other adverse events, health-related quality of life, healthcare costs and mortality. To achieve 90% power (alpha=0.05) for the primary outcome, data from 720 recruitments are required. A mixed-methods approach will be employed to explore implementation contexts from the perspective of clinicians and healthcare purchasers.Ethics and disseminationEthics approval has been provided by Children’s Health Queensland Hospital and Health Service Human Research Ethics Committee (HREC) (HREC/22/QCHQ/81744) and the University of Queensland HREC (2022/HE000196) with subsequent governance approval at all sites. Informed consent is required from the substitute decision-maker or legal guardian prior to participation. In addition, consent may also be obtained from mature minors, depending on the legislative requirements of the study site. The primary trial and substudies will be written by the investigators and published in peer-reviewed journals. The findings will also be disseminated through local health and clinical trial networks by investigators and presented at conferences.Trial registration numberACTRN12622000499785.
Emergency department workforces’ experiences and perceptions of well-being from an international perspective: a scoping review
Authors: Swancott Lucy , Armstrong Natalie , Roland Damian , Walters Helen L , Kirk Kate 
Published: July 2024

ObjectivesTo identify and present the available evidence regarding workforce well-being in the emergency department.DesignScoping review.SettingThe emergency department (ED).Data sourcesCINAHL, MEDLINE, APA PsycINFO and Web of Science were searched with no publication time parameters. The reference lists of articles selected for full-text review were also screened for additional papers.Eligibility criteria for study selectionAll peer-reviewed, empirical papers were included if: (1) participants included staff-based full-time in the ED, (2) ED workforce well-being was a key component of the research, (3) English language was available and (4) the main focus was not burnout or other mental illness-related variables.ResultsThe search identified 6109 papers and 34 papers were included in the review. Most papers used a quantitative or mixed methods survey design, with very limited evidence using in-depth qualitative methods to explore ED workforce well-being. Interventions accounted for 41% of reviewed studies. Findings highlighted pressing issues with ED workforce well-being, contributed to by a range of interpersonal, organisational and individual challenges (eg, high workloads, lack of support). However, the limited evidence base, tenuous conceptualisations and links to well-being in existing literature mean that the findings were neither consistent nor conclusive.ConclusionsThis scoping review highlights the need for more high-quality research to be conducted, particularly using qualitative methods and the development of a working definition of ED workforce well-being.
Searching for the open flavor tetraquark $$T_{c\bar{s}0}(2900)^{++}$$ in the process $$B^+\rightarrow K^+ D^+ D^-$$
Authors: Duan Man-Yu , Wang En , Chen Dian-Yong 
Published: July 2024

AbstractInspired by recent observations of$$T_{c\bar{s}0}(2900)^0$$Tcs¯0(2900)0in the$$D_s^+ \pi ^-$$Ds+π-invariant mass distribution of$$B^0 \rightarrow \bar{D}^0 D_s^+ \pi ^-$$B0→D¯0Ds+π-decay and$$T_{c\bar{s}0}(2900)^{++}$$Tcs¯0(2900)++in the$$D_s^+ \pi ^+$$Ds+π+invariant mass distribution of$$B^+ \rightarrow D^- D_s^+ \pi ^+$$B+→D-Ds+π+decay, we investigate the$$T_{c\bar{s}0}(2900)^{++}$$Tcs¯0(2900)++contribution to the$$B^+ \rightarrow K^+ D^+ D^-$$B+→K+D+D-decay in a molecular scenario, where we consider$$T_{c\bar{s}0}(2900)^{++}$$Tcs¯0(2900)++as a$$D^{*+} K^{*+}$$D∗+K∗+molecular state. Our estimations indicate that the fit fraction of$$T_{c\bar{s}0}(2900)^{++}$$Tcs¯0(2900)++in the$$B^+ \rightarrow K^+ D^+ D^-$$B+→K+D+D-is about$$13\%$$13%, and its signal is visible in the$$D^+ K^+$$D+K+invariant mass distribution. With the involvement of$$T_{c\bar{s}0}(2900)^{++}$$Tcs¯0(2900)++, the fit fractions of$$\chi _{c0}(3915)$$χc0(3915)and$$\chi _{c2}(3930)$$χc2(3930)may be much different with the ones obtained by the amplitude analysis in Ref. [Aaij et al. Phys. Rev. D 102:112003, 2020], which may shed light on the long standing puzzle of$$\chi _{c0}(3915)$$χc0(3915)as the conventional charmonium.
EPAC1 and 2 inhibit K+ currents via PLC/PKC and NOS/PKG pathways in rat ventricular cardiomyocytes
Authors: Boileve Arthur , Romito Olivier , Hof Thomas , Levallois Aurélia , Brard Laura , d’Hers Sarah , Fouchet Alexandre , Simard Christophe , Guinamard Romain , Brette Fabien , Sallé Laurent 
Published: July 2024

The exchange protein directly activated by cAMP (EPAC) has been implicated in cardiac proarrhythmic signaling pathways including spontaneous diastolic Ca2+ leak from sarcoplasmic reticulum and increased action potential duration (APD) in isolated ventricular cardiomyocytes. The action potential (AP) lengthening following acute EPAC activation is mainly due to a decrease of repolarizing steady-state K+ current (IKSS) but the mechanisms involved remain unknown. This study aimed to assess the role of EPAC1 and EPAC2 in the decrease of IKSS and to investigate the underlying signaling pathways. AP and K+ currents were recorded with the whole cell configuration of the patch-clamp technique in freshly isolated rat ventricular myocytes. EPAC1 and EPAC2 were pharmacologically activated with 8-(4-chlorophenylthio)-2′- O-methyl-cAMP acetoxymethyl ester (8-CPTAM, 10 µmol/L) and inhibited with R-Ce3F4 and ESI-05, respectively. Inhibition of EPAC1 and EPAC2 significantly decreased the effect of 8-CPTAM on APD and IKSS showing that both EPAC isoforms are involved in these effects. Unexpectedly, calmodulin-dependent protein kinase II (CaMKII) inhibition by AIP or KN-93, and Ca2+ chelation by intracellular BAPTA, did not impact the response to 8-CPTAM. However, inhibition of PLC/PKC and nitric oxide synthase (NOS)/PKG pathways partially prevents the 8-CPTAM-dependent decrease of IKSS. Finally, the cumulative inhibition of PKC and PKG blocked the 8-CPTAM effect, suggesting that these two actors work along parallel pathways to regulate IKSS upon EPAC activation. On the basis of such findings, we propose that EPAC1 and EPAC2 are involved in APD lengthening by inhibiting a K+ current via both PLC/PKC and NOS/PKG pathways. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy. NEW & NOTEWORHTY Exchange protein directly activated by cAMP (EPAC) proteins modulate ventricular electrophysiology at the cellular level. Both EPAC1 and EPAC2 isoforms participate in this effect. Mechanistically, PLC/PKC and nitric oxide synthase (NO)/PKG pathways are involved in regulating K+ repolarizing current whereas the well-known downstream effector of EPAC, calmodulin-dependent protein kinase II (CaMKII), does not participate. This may have pathological implications since EPAC is upregulated in diseases such as cardiac hypertrophy. Thus, EPAC inhibition may be a new approach to prevent arrhythmias under pathological conditions.
The future of data analysis is now: Integrating generative AI in neuroimaging methods development
Authors: DuPre Elizabeth , Poldrack Russell Alan 
Published: July 2024

Abstract
In this perspective, we highlight how emerging artificial intelligence tools are likely to impact the experiences of researchers conducting computational fMRI analyses. While calls for the automatization of statistical procedures date back at least to the inception of “data science” as a field, generative artificial intelligence offers new opportunities to advance field practice. We highlight how these tools are poised to impact both new neuroimaging methods development in areas such as image quality control and in day-to-day practice when generating analysis code. We argue that considering generative artificial intelligence as a catalyst for computational neuroscience—rather than as unique tools in their own right—can substantially improve its positioning in the research ecosystem. In particular, we argue that generative artificial intelligence will reinforce the importance of existing open science initiatives, rather than supplanting them. Overall, we call for clearer metrics by which neuroimaging results—whether generated by individual research teams or by generative artificial intelligence technologies—can be meaningfully compared.
Enhanced procoagulant activity of select hemophilia B causing factor IX variants with emicizumab
Authors: Lee Kyumin , Chau Julia Q. , Suber Yani B. , Sternberg Anna R. , Pishko Allyson M. , George Lindsey A. , Bhoj Vijay G. , Doshi Bhavya S. , Samelson-Jones Benjamin J. 
Published: July 2024

Abstract
Emicizumab improves the procoagulant activity of select loss-of-function factor IX (FIX) variants with likely dysfunctional assembly of the intrinsic Xase complex, resulting in hemophilia B (HB). FVIII mimetics may represent an alternative nonfactor therapy for select patients with HB.
RBCs regulate platelet function and hemostasis under shear conditions through biophysical and biochemical means
Authors: Jiang Debbie , Houck Katie L. , Murdiyarso Lydia , Higgins Harrison , Rhoads Nicole , Romero Sophia K. , Kozar Rosemary , Nascimbene Angelo , Gernsheimer Terry B. , Sanchez Zyrina Alura C. , Ramasubramanian Anand K. , Adili Reheman , Dong Jing-fei 
Published: July 2024

Abstract
Red blood cells (RBCs) have been hypothesized to support hemostasis by facilitating platelet margination and releasing platelet-activating factors such as adenosine 5′-diphosphate (ADP). Significant knowledge gaps remain regarding how RBCs influence platelet function, especially in (patho)physiologically relevant hemodynamic conditions. Here, we present results showing how RBCs affect platelet function and hemostasis in conditions of anemia, thrombocytopenia, and pancytopenia and how the biochemical and biophysical properties of RBCs regulate platelet function at the blood and vessel wall interface and in the fluid phase under flow conditions. We found that RBCs promoted platelet deposition to collagen under flow conditions in moderate (50 × 103/μL) but not severe (10 × 103/μL) thrombocytopenia in vitro. Reduction in hematocrit by 45% increased bleeding in mice with hemolytic anemia. In contrast, bleeding diathesis was observed in mice with a 90% but not with a 60% reduction in platelet counts. RBC transfusion improved hemostasis by enhancing fibrin clot formation at the site of vascular injury in mice with severe pancytopenia induced by total body irradiation. Altering membrane deformability changed the ability of RBCs to promote shear-induced platelet aggregation. RBC-derived ADP contributed to platelet activation and aggregation in vitro under pathologically high shear stresses, as observed in patients supported by left ventricular assist devices. These findings demonstrate that RBCs support platelet function and hemostasis through multiple mechanisms, both at the blood and vessel wall interface and in the fluidic phase of circulation.
High-sensitivity whole-mount in situ Hybridization of Mouse Oocytes and Embryos Visualizes the Super-resolution Structures and Distributions of mRNA Molecules
Authors: Sanada Takahiro , Kotani Tomoya 
Published: July 2024

AbstractMammalian oocytes accumulate more than ten thousand mRNAs, of which three to four thousand mRNAs are translationally repressed. The timings and sites of translational activation of these dormant mRNAs are crucial for promoting oocyte maturation and embryonic development. How these mRNAs are accumulated and distributed in oocytes is therefore a fundamental issue to be explored. A method that enables visualization of mRNA molecules with high resolution in a simple manner would be valuable for understanding how oocytes accumulate and regulate the dormant mRNAs. We have developed a highly sensitive whole-mount in situ hybridization method using in vitro-synthesized RNA probes and the tyramide signal amplification (TSA) system optimized for mouse oocytes and embryos. By using this method, Pou5f1/Oct4, Emi2, and cyclin B1 mRNAs were detected in immature oocytes and 2-cell stage embryos. Confocal microscopy showed that these mRNAs formed granular structures in the oocyte cytoplasm. The structures of Pou5f1/Oct4 and cyclin B1 mRNAs persisted in 2-cell stage embryos. Pou5f1/Oct4 RNA granules exhibited a solid-like property in immature oocytes and became liquid-like droplets in 2-cell stage embryos. Double-staining of cyclin B1 mRNA with Emi2 or Pou5f1/Oct4 mRNA revealed that these mRNAs were distributed as different RNA granules without overlapping each other and that the size of cyclin B1 RNA granules tended to be larger than that of Emi2 RNA granules. The structures and distribution patterns of these mRNAs were further analyzed by N-SIM super-resolution microscopy. This analysis revealed that the large-sized RNA granules consist of many small-sized granules, suggesting the accumulation and regulation of dormant mRNAs as basal-sized RNA granules. The method established in this study can easily visualize the structure and distribution of mRNAs accumulated in mammalian oocytes and embryos with high sensitivity and super-resolution. This method is useful for investigating the cellular and molecular mechanisms of translational control of mRNAs by which maturation and early developmental processes are promoted.
Enhancing growth and bioactive metabolites characteristics in Mentha pulegium L. via silicon nanoparticles during in vitro drought stress
Authors: El-Naggar Hany M. , Osman Amira R. 
Published: July 2024

AbstractThe development and production of secondary metabolites from priceless medicinal plants are restricted by drought stress. Mentha pulegium L. belongs to the Lamiaceae family and is a significant plant grown in the Mediterranean region for its medicinal and aesthetic properties. This study investigated the effects of three polyethylene glycol (PEG) (0, 5, and 10%) as a drought stress inducer and four silicon nanoparticle (SiNP) (0, 25, 50, and 100 ppm) concentrations as an elicitor to overcome the adverse effect of drought stress, on the growth parameters and bioactive chemical composition of M. pulegium L. plants grown in vitro. The experiment was performed as a factorial experiment using a completely randomized design (CRD) consisting of 12 treatments with two factors (3 PEG × 4 SiNPs concentrations), 6 replicates were used for each treatment for a total of 72 experimental units.The percentage of shoot formation was inversely proportional to the PEG concentration; for the highest PEG concentration, the lowest percentage of shoot formation (70.26%) was achieved at 10% PEG. SiNPs at 50 ppm enhanced shoot formation, the number of shoots, shoot height, fresh and dry weight, rosmarinic acid, total phenols, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity. The methanol extract from M. pulegium revealed the presence of significant secondary metabolites using gas chromatography‒mass spectrometry (GC–MS). The principal constituents of the extract were limonene (2.51, 2.99%), linalool (3.84, 4.64%), geraniol (6.49, 8.77%), menthol (59.73, 65.43%), pulegone (3.76, 2.76%) and hexadecanoic acid methyl ester or methyl palmitate (3.2, 4.71%) for the 0 ppm SiNPs, PEG 0% and 50 ppm SiNPs, and PEG 10%, respectively. Most of the chemical components identified by GC‒MS in the methanol extract were greater in the 50 ppm SiNP and 10% PEG treatment groups than in the control group. SiNP improves drought tolerance by regulating biosynthesis and accumulating some osmolytes and lessens the negative effects of polyethylene glycol-induced drought stress.Based on the results, the best treatment for most of the parameters was 50 ppm SiNPs combined with 10% PEG, the morphological and chemical characteristics were inversely proportional to the PEG concentration, as the highest PEG concentration (10%) had the lowest results. Most parameters decreased at the highest SiNP concentration (100 ppm), except for the DPPH scavenging percentage, as there was no significant difference between the 50 and 100 ppm SiNPs.
Complete plastome genomes of three medicinal heliotropiaceae species: comparative analyses and phylogenetic relationships
Authors: Alawfi Mohammad S. , Alzahrani Dhafer A. , Albokhari Enas J. 
Published: July 2024

Abstract
Background
Heliotropiaceae is a family of the order Boraginales and has over 450 species. The members of the family Heliotropiaceae have been widely reported to be used in traditional medicine Over time, the classification of Heliotropiaceae has remained uncertain and has moved from family to subfamily, or conversely.

Results
In the present study, we sequenced, analyzed, and compared the complete plastomes of Euploca strigosa, Heliotropium arbainense, and Heliotropium longiflorum with the genomes of related taxa. The lengths of the plastomes of E. strigosa, H. arbainense, and H. longiflorum were 155,174 bp, 154,709 bp, and 154,496 bp, respectively. Each plastome consisted of 114 genes: 80 protein-coding genes, 4 ribosomal RNA genes, and 30 transfer RNA genes. The long repeats analysis indicated that reverse, palindromic, complement and forward repeats were all found in the three plastomes. The simple repeats analysis showed that the plastomes of E. strigosa, H. arbainense, and H. longiflorum contained 158, 165, and 151 microsatellites, respectively. The phylogenetic analysis confirmed two major clades in the Boraginales: clade I comprised Boraginaceae, while clade II included Heliotropiaceae, Ehretiaceae, Lennoaceae, and Cordiaceae. Inside the family Heliotropiaceae, E. strigosa is nested within the Heliotropium genus.

Conclusions
This study expands our knowledge of the evolutionary relationships within Heliotropiaceae and offers useful genetic resources.
Apolipoprotein E E3/E4 genotype is associated with an increased risk of premature coronary artery disease
Authors: Li Youqian , Zhong Wei , Huang Changjing , Peng Junyin , Li Hanlin 
Published: July 2024

Abstract
Objective
Dyslipidemia is one of the causes of coronary heart disease (CAD), and apolipoprotein E (APOE) gene polymorphism affects lipid levels. However, the relationship between APOE gene polymorphisms and premature CAD (PCAD, male CAD patients with ≤ 55 years old and female with ≤ 65 years old) risk had different results in different studies. The aim of this study was to assess this relationship and to further evaluate the relationship between APOE gene polymorphisms and PCAD risk in the Hakka population.

Methods
This study retrospectively analyzed 301 PCAD patients and 402 age matched controls without CAD. The APOE rs429358 and rs7412 polymorphisms were genotyped by polymerase chain reaction (PCR) -chip technique. The distribution of APOE genotypes and alleles between the case group and the control group was compared. The relationship between APOE genotypes and PCAD risk was obtained by logistic regression analysis.

Results
The frequency of the APOE ɛ3/ɛ4 genotype (18.9% vs. 10.2%, p = 0.001) and ε4 allele (11.1% vs. 7.0%, p = 0.007) was higher in the PCAD patients than that in controls, respectively. PCAD patients with ɛ2 allele had higher TG level than those with ɛ3 allele, and controls carried ɛ2 allele had higher HDL-C level and lower LDL-C level than those carried ɛ3 allele. Regression logistic analysis showed that BMI ≥ 24.0 kg/m2 (BMI ≥ 24.0 kg/m2 vs. BMI 18.5–23.9 kg/m2, OR: 1.763, 95% CI: 1.235–2.516, p = 0.002), history of smoking (Yes vs. No, OR: 5.098, 95% CI: 2.910–8.930, p < 0.001), ɛ3/ɛ4 genotype (ɛ3/ɛ4 vs. ɛ3/ɛ3, OR: 2.203, 95% CI: 1.363–3.559, p = 0.001), ε4 allele (ε4 vs. ε3, OR: 2.125, 95% CI: 1.333–3.389, p = 0.002), and TC level (OR: 1.397, 95% CI: 1.023–1.910, p = 0.036) were associated with PCAD.

Conclusions
In summary, BMI ≥ 24.0 kg/m2, history of smoking, APOE ɛ3/ɛ4 genotype, and TC level were independent risk factors for PCAD. It means that young individuals who are overweight, have a history of smoking, and carried APOE ɛ3/ɛ4 genotype had increased risk of PCAD.