bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scaffold and scaffold‐free self‐assembled systems in regenerative medicine
Authors: Thomas Dilip , Gaspar Diana , Sorushanova Anna , Milcovich Gesmi , Spanoudes Kyriakos , Mullen Anne Maria , O'Brien Timothy , Pandit Abhay , Zeugolis Dimitrios I. 
Published: October 2015

ABSTRACTSelf‐assembly in tissue engineering refers to the spontaneous chemical or biological association of components to form a distinct functional construct, reminiscent of native tissue. Such self‐assembled systems have been widely used to develop platforms for the delivery of therapeutic and/or bioactive molecules and various cell populations. Tissue morphology and functional characteristics have been recapitulated in several self‐assembled constructs, designed to incorporate stimuli responsiveness and controlled architecture through spatial confinement or field manipulation. In parallel, owing to substantial functional properties, scaffold‐free cell‐assembled devices have aided in the development of functional neotissues for various clinical targets. Herein, we discuss recent advancements and future aspirations in scaffold and scaffold‐free self‐assembled devices for regenerative medicine purposes. Biotechnol. Bioeng. 2016;113: 1155–1163. © 2015 Wiley Periodicals, Inc.
Analogues of Cis‐ and Transplatin with a Rich Solution Chemistry: cis‐[PtCl2(NH3)(1‐MeC‐N3)] and trans‐[PtI2(NH3)(1‐MeC‐N3)]
Authors: Siebel Sabine , Dammann Claudia , Sanz Miguel Pablo J. , Drewello Thomas , Kampf Gunnar , Teubner Natascha , Bednarski Patrick J. , Freisinger Eva , Lippert Bernhard 
Published: October 2015

AbstractMono(nucleobase) complexes of the general composition cis‐[PtCl2(NH3)L] with L=1‐methylcytosine, 1‐MeC (1 a) and L=1‐ethyl‐5‐methylcytosine, as well as trans‐[PtX2(NH3)(1‐MeC)] with X=I (5 a) and X=Br (5 b) have been isolated and were characterized by X‐ray crystallography. The Pt coordination occurs through the N3 atom of the cytosine in all cases. The diaqua complexes of compounds 1 a and 5 a, cis‐[Pt(H2O)2(NH3)(1‐MeC)]2+ and trans‐[Pt(H2O)2(NH3)(1‐MeC)]2+, display a rich chemistry in aqueous solution, which is dominated by extensive condensation reactions leading to μ‐OH‐ and μ‐(1‐MeC−‐N3,N4)‐bridged species and ready oxidation of Pt to mixed‐valence state complexes as well as diplatinum(III) compounds, one of which was characterized by X‐ray crystallography: h,t‐[{Pt(NH3)2(OH)(1‐MeC−‐N3,N4)}2](NO3)2⋅2 [NH4](NO3)⋅2 H2O. A combination of 1H NMR spectroscopy and ESI mass spectrometry was applied to identify some of the various species present in solution and the gas phase, respectively. As it turned out, mass spectrometry did not permit an unambiguous assignment of the structures of +1 cations due to the possibilities of realizing multiple bridging patterns in isomeric species, the occurrence of different tautomers, and uncertainties regarding the Pt oxidation states. Additionally, compound 1 a was found to have selective and moderate antiproliferative activity for a human cervix cancer line (SISO) compared to six other human cancer cell lines.
Six, Seven or Eight Coordinate FeII, CoII or NiII Complexes of Amide‐Appended Tetraazamacrocycles for ParaCEST Thermometry
Authors: Olatunde Abiola O. , Bond Christopher J. , Dorazio Sarina J. , Cox Jordan M. , Benedict Jason B. , Daddario Michael D. , Spernyak Joseph A. , Morrow Janet R. 
Published: October 2015

AbstractFeII, CoII and NiII complexes of two tetraazamacrocycles (1,4,8,11‐tetrakis(carbamoylmethyl)‐1,4,8,11‐tetraazacyclotetradecane (L1) and 1,4,7,10‐tetrakis(carbamoylmethyl)‐1,4,7,10‐tetraazacyclododecane (L2) show promise as paraCEST agents for registration of temperature (paraCEST=paramagnetic chemical exchange saturation transfer). The FeII, CoII and NiII complexes of L1 show up to four CEST peaks shifted ≤112 ppm, whereas analogous complexes of L2 show only a single CEST peak at ≤69 ppm. Comparison of the temperature coefficients (CT) of the CEST peaks of [Co(L2)]2+, [Fe(L2)]2+, [Ni(L1)]2+ and [Co(L1)]2+ showed that a CEST peak of [Co(L1)]2+ gave the largest CT (−0.66 ppm oC−1 at 4.7 T). NMR spectral and CEST properties of these complexes correspond to coordination complex symmetry as shown by structural data. The [Ni(L1)]2+ and [Co(L1)]2+ complexes have a six‐coordinate metal ion bound to the 1‐, 4‐amide oxygen atoms and four nitrogen atoms of the tetraazamacrocycle. The [Fe(L2)]2+ complex has an unusual eight‐coordinate FeII bound to four amide oxygen atoms and four macrocyclic nitrogen atoms. For [Co(L2)]2+, one structure has seven‐coordinate CoII with three bound amide pendents and a second structure has a six‐coordinate CoII with two bound amide pendents.
Redox‐Robust Pentamethylferrocene Polymers and Supramolecular Polymers, and Controlled Self‐Assembly of Pentamethylferricenium Polymer‐Embedded Ag, AgI, and Au Nanoparticles
Authors: Gu Haibin , Ciganda Roberto , Castel Patricia , Vax Amélie , Gregurec Danijela , Irigoyen Joseba , Moya Sergio , Salmon Lionel , Zhao Pengxiang , Ruiz Jaime , Hernández Ricardo , Astruc Didier 
Published: October 2015

AbstractWe report the first pentamethylferrocene (PMF) polymers and the redox chemistry of their robust polycationic pentamethylferricenium (PMFium) analogues. The PMF polymers were synthesized by ring‐opening metathesis polymerization (ROMP) of a PMF‐containing norbornene derivative by using the third‐generation Grubbs ruthenium metathesis catalyst. Cyclic voltammetry studies allowed us to determine confidently the number of monomer units in the polymers through the Bard–Anson method. Stoichiometric oxidation by using ferricenium hexafluorophosphate quantitatively and instantaneously provided fully stable (even in aerobic solutions) blue d5 FeIII metallopolymers. Alternatively, oxidation of the PMF‐containing polymers was conducted by reactions with AgI or AuIII, to give PMFium polymer‐embedded Ag and Au nanoparticles (NPs). In the presence of I2, oxidation by using AgI gave polymer‐embedded Ag/AgI NPs and AgNPs at the surface of AgI NPs. Oxidation by using AuIII also produced an AuI intermediate that was trapped and characterized. Engineered single‐electron transfer reactions of these redox‐robust nanomaterial precursors appear to be a new way to control their formation, size, and environment in a supramolecular way.
Dioxygen‐Mediated Decarbonylative CH Alkylation of Heteroaromatic Bases with Aldehydes
Authors: Paul Subhasis , Guin Joyram 
Published: October 2015

AbstractAn operationally simple and economical method for the direct alkylation of heteroaromatic bases employing readily available aldehydes as alkyl radical precursors and molecular oxygen as a reagent is presented. This simple transformation demonstrates a broad substrate scope with respect to aldehydes and nitrogen heterocycles, enabling the introduction of several medicinally important yet challenging alkyl moieties, such as ethyl, isopropyl, tert‐butyl, and cyclohexyl to the different classes of heterocyclic bases in good to excellent yields.
3‐Amidocoumarins as Potential Multifunctional Agents against Neurodegenerative Diseases
Authors: Matos Maria João , Rodríguez‐Enríquez Fernanda , Borges Fernanda , Santana Lourdes , Uriarte Eugenio , Estrada Martín , Rodríguez‐Franco María Isabel , Laguna Reyes , Viña Dolores 
Published: October 2015

AbstractMonoamine oxidase (MAO) generates reactive oxygen species (ROS), which cause neuronal cell death, causing neurodegeneration. Agents that are able to concurrently inhibit MAO and scavenge free radicals represent promising multifunctional neuroprotective agents that could be used to delay or slow the progression of neurodegenerative diseases. In this work, variously substituted 3‐amidocoumarins are described that exert neuroprotection in vitro against hydrogen peroxide in rat cortical neurons, as well as antioxidant activity in a 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH⋅) radical scavenging assay. Selective and reversible inhibitors of the MAO‐B isoform were identified. Interestingly, in the case of the 3‐benzamidocoumarins, substitution at position 4 with a hydroxy group abolishes MAO‐B activity, but the compounds remain active in the neuroprotection model. Further evaluation of 3‐heteroarylamide derivatives indicates that it is the nature of the heterocycle that determines the neuroprotective effects. Evaluation in a parallel artificial membrane permeability assay (PAMPA) highlighted the need to further improve the blood–brain barrier permeability of this compound class. However, the compounds described herein adhere to Lipinski′s rule of five, suggesting that this novel scaffold has desirable properties for the development of potential drug candidates.
Fish consumption as a driver of risk-management decisions and human health–based water quality criteria
Authors: Judd Nancy , Lowney Yvette , Anderson Paul , Baird Suzanne , Bay Steven M. , Breidt Jay , Buonanduci Michele , Dong Zhao , Essig Don , Garry Michael R. , Jim Rebecca C. , Kirkwood Gemma , Moore Shelly , Niemi Cheryl , O'Rourke Rory , Ruffle Betsy , Schaider Laurel A. , Vidal-Dorsch Doris E. 
Published: October 2015

Abstract

The use and interpretation of fish consumption surveys and interviews, the application of fish consumption rates for sediment evaluation and cleanup, and the development of human health water quality criteria (HH WQC) are complex and interrelated issues. The present article focuses on these issues using examples from the United States, although the issues may be relevant for other countries. Some key considerations include the fact that there are many types of fish consumption surveys (e.g., 24-h recall surveys, food frequency questionnaires, creel surveys), and these surveys have different advantages and limitations. Identification of target populations for protection, identification of the species and quantities of fish consumed, and determination of bioaccumulation assumptions are important factors when developing water quality and sediment screening levels and standards. Accounting for the cultural importance of fish consumption for some populations is an even more complex element. Discussions about HH WQC often focus only on the fish consumption rate and may not have broad public input. Some states are trying to change this through extensive public participation efforts and use of probabilistic approaches to derive HH WQC. Finally, there are limits to what WQC can achieve. Solutions beyond the establishment of WQC that target toxics reduction from other sources may provide the greatest improvements to water quality and reductions in human health risks in the future. Environ Toxicol Chem 2015;34:2427–2436. © 2015 SETAC
Development of a Taxonomy of Keywords for Engineering Education Research
Authors: Finelli Cynthia J. , Borrego Maura , Rasoulifar Golnoosh 
Published: October 2015

AbstractThe diversity of engineering education research provides an opportunity for cross‐fertilization of ideas and creativity, but it also can result in fragmentation of the field and duplication of effort. One solution is to establish a standardized taxonomy of engineering education terms to map the field and communicate and connect research initiatives. This report describes the process for developing such a taxonomy, the EER Taxonomy. Although the taxonomy focuses on engineering education research in the United States, inclusive efforts have engaged 266 individuals from 149 cities in 30 countries during one multiday workshop, seven conference sessions, and several other virtual and in‐person activities. The resulting taxonomy comprises 455 terms arranged in 14 branches and six levels. This taxonomy was found to satisfy four criteria for validity and reliability: (1) keywords assigned to a set of abstracts were reproducible by multiple researchers, (2) the taxonomy comprised terms that could be selected as keywords to fully describe 243 articles in three journals, (3) the keywords for those 243 articles were evenly distributed across the branches of the taxonomy, and (4) the authors of 31 conference papers agreed with 90% of researcher‐assigned keywords. This report also describes guidelines developed to help authors consistently assign keywords for their articles by encouraging them to choose terms from three categories: (1) context/focus/topic, (2) purpose/target/motivation, and (3) research approach.
“I Am an Engineer AND”: A Mixed Methods Study of Socially Engaged Engineers
Authors: Litchfield Kaitlin , Javernick‐Will Amy 
Published: October 2015

AbstractBackgroundSocially engaged engineering activities such as community development have grown rapidly in popularity. The engineers who participate in these activities seem more diverse and more broadly interested than the larger population of engineers in the United States.Purpose/HypothesisThis article compares the personal attributes, specifically personality traits and motivations to study engineering, of members of a prominent engineering service organization, Engineers Without Borders (EWB‐USA), with the attributes of engineers who were not members.Design/MethodUsing an exploratory, sequential mixed methods approach that combined variable‐oriented analysis of interviews and focus groups with multiple logistic regression models from responses to a national survey, we compared engineers' personality traits and motivations to study engineering.ResultsResults indicate that both EWB‐USA members and nonmembers exhibit strong personality traits for conscientiousness and emotional stability. Both members and nonmembers have similar intrinsic engineering interests and motivations. EWB‐USA members have significantly stronger personality traits for openness to experience and agreeableness, stronger motivations for social good, and broader interests than do nonmembers.ConclusionsEWB‐USA members have personal attributes that match those of other engineers, but they have broader interests and motivations than do other engineers. These differences suggest including socially engaged engineering activities in engineering may increase the number and broaden the diversity of engineering students and practitioners.
Morphology of sealant/enamel interface after surface treatment with bioactive glass
Authors: de Carvalho Panzeri Pires‐de‐Souza Fernanda , Silveira Renata Espíndola , Abuna Gabriel , Chinelatti Michelle Alexandra , Alandia‐Román Carla Cecilia , Sinhoreti Mario Alexandre Coelho 
Published: October 2015

ABSTRACTThe purpose of this study was to analyze, by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM), the morphology of sealant/enamel interface after surface treatment with Biosilicate. Before pits and fissures sealing, the occlusal surfaces of 10 sound human molars were sectioned perpendicularly at the fissures in order to obtain three slices for each tooth. Slices were randomly assigned into three groups (n = 10) according to sealing protocol: Group 1‐ Acid etching + Biosilicate + glass ionomer‐based sealant (Clinpro XT Varnish, 3M ESPE); Group 2‐ Acid etching + glass ionomer‐based sealant (Clinpro XT Varnish, 3M ESPE); Group 3‐ No sealing. All slices were subjected to thermal cycling (5,000 cycles; 5–55°C; dwell time: 30s). Half of the slices from each group (n = 5) were analyzed by CLSM and the other half by SEM. Groups 1 and 2 were also submitted to EDS analysis and their data were evaluated by Two‐Way ANOVA e Tukey's test (α=5%). EDS data analysis showed higher amounts of silicon (Si) ions than calcium (Ca) ions in Group 1 (P < 0.05); Group 2 presented higher amounts (P < 0.05) of Ca ions than Si ions. It may be concluded that the use of Biosilicate for surface treatment did not affect the morphology of glass ionomer‐based sealant/enamel interfaces. Microsc. Res. Tech. 78:1062–1068, 2015. © 2015 Wiley Periodicals, Inc.
Genetically‐defined ovarian cancer mouse models
Authors: Morin Patrice J , Weeraratna Ashani T 
Published: October 2015

AbstractEpithelial ovarian cancer (EOC), the deadliest of gynaecological cancers, is a disease that remains difficult to detect early and treat efficiently. A significant challenge for researchers in the field is that the aetiology of EOC and the molecular pathways important for its development are poorly understood. Moreover, precursor lesions have not been readily identifiable, making the mechanisms of EOC progression difficult to delineate. In order to address these issues, several genetically‐defined ovarian mouse models have been generated in the past 15 years. However, because of the recent suggestion that most EOCs may not originate from the ovarian surface ‘epithelium’, but from other tissues of the female genital tract, some models may need to be re‐evaluated within this new paradigm. In this review, we examine several genetically‐defined EOC models and discuss how the new paradigm may explain some of the features of these models. A better understanding of the strengths and limitations of the current EOC mouse models will undoubtedly allow us to utilize these tools to better understand the biology of the disease and develop new approaches for EOC prevention, detection, and treatment. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Over‐expression of prothymosin‐α antagonizes TGFβ signalling to promote the development of emphysema
Authors: Su Bing‐Hua , Tseng Yau‐Lin , Shieh Gia‐Shing , Chen Yi‐Cheng , Wu Pensee , Shiau Ai‐Li , Wu Chao‐Liang 
Published: October 2015

AbstractEmphysema, a major consequence of chronic obstructive pulmonary disease (COPD), is characterized by the permanent airflow restriction resulting from enlargement of alveolar airspace and loss of lung elasticity. Transforming growth factor‐β (TGFβ) signalling regulates the balance of matrix metalloproteinase (MMP)/tissue inhibitor of matrix metalloproteinase (TIMP) to control matrix homeostasis. Patients with COPD have dysregulated TGFβ signalling and reduced histone deacetylase (HDAC) activity through epigenetic up‐regulation of histone acetylation in the promoters of pro‐inflammatory genes. However, the potential link between decreased HDAC activity and dysregulated TGFβ signalling in emphysema pathogenesis remains to be determined. Prothymosin α (ProT), a highly conserved acidic nuclear protein, plays a role in the acetylation of histone and non‐histone proteins. The aim of this study was to test the hypothesis that ProT inhibits TGFβ–Smad signalling through Smad7, thereby contributing to emphysema pathogenesis. We show that ProT enhances Smad7 acetylation by decreasing its association with HDAC and thereby down‐regulates TGFβ–Smad signalling. ProT caused an imbalance between MMP and TIMP through acetylated Smad7 in favour of MMP expression. In addition to interfering with R‐Smad activation and targeting receptors for degradation in the cytoplasm, acetylated Smad7 potentiated by ProT competitively antagonized binding of the pSmad2/3–Smad4 complex to the TIMP‐3 promoter, resulting in reduced TIMP‐3 expression. These effects were detected in ProT‐over‐expressing cells, lungs of ProT transgenic mice displaying an emphysema phenotype and in emphysema patients. Importantly, increased Smad7 and reduced TIMP‐3 were found in the lungs of emphysema patients and mice with cigarette smoke extract (CSE)‐induced emphysema. Such effects could be abrogated by silencing endogenous ProT expression. Collectively, our results uncover acetylated Smad7 regulated by ProT as an important determinant in dysregulated TGFβ signalling that contributes to emphysema pathogenesis. Copyright © 2015 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Endocrine Dysfunction in Diamond–Blackfan Anemia (DBA): A Report from the DBA Registry (DBAR)
Authors: Lahoti Amit , Harris Yael T , Speiser Phyllis W , Atsidaftos Evangelia , Lipton Jeffrey M , Vlachos Adrianna 
Published: October 2015

BackgroundDiamond–Blackfan anemia (DBA) is a rare inherited bone marrow failure syndrome. The mainstays of treatment involve chronic red cell transfusions, long‐term glucocorticoid therapy, and stem cell transplantation. Systematic data concerning endocrine function in DBA are limited. We studied patients in the DBA Registry (DBAR) of North America to assess the prevalence of various endocrinopathies.ProcedureIn a pilot study, retrospective data were collected for 12 patients with DBA. Subsequently, patients with DBA aged 1–39 years were recruited prospectively. Combined, 57 patients were studied; 38 chronically transfused, 12 glucocorticoid‐dependent, and seven in remission. Data were collected on anthropometric measurements, systematic screening of pituitary, thyroid, parathyroid, adrenal, pancreatic, and gonadal function, and ferritin levels. Descriptive statistics were tabulated and group differences were assessed.ResultsFifty‐three percent of patients had ≥1 endocrine disorder, including adrenal insufficiency (32%), hypogonadism (29%), hypothyroidism (14%), growth hormone dysfunction (7%), diabetes mellitus (2%), and/or diabetes insipidus (2%). Ten of the 33 patients with available heights had height standard deviation less than −2. Low 25‐hydroxy vitamin D (25(OH)D) levels were present in 50%. A small proportion also had osteopenia, osteoporosis, or hypercalciuria. Most with adrenal insufficiency were glucocorticoid dependent; other endocrinopathies were more common in chronically transfused patients.ConclusionsEndocrine dysfunction is common in DBA, as early as the teenage years. Although prevalence is highest in transfused patients, patients taking glucocorticoids or in remission also have endocrine dysfunction. Longitudinal studies are needed to better understand the etiology and true prevalence of these disorders.
Syntheses, Structures, and Luminescent Properties of d10 Coordination Polymers with 2‐(4‐Carboxyphenyl)‐imidazo[4, 5‐f]‐1, 10‐phenanthroline and 1, 3‐Benzenedicarboxylate Derivatives
Authors: Fang Jie‐Fang , Cheng Jing‐Xiang , Huang Shu‐Ting , Zhang Jing , Ni Chang‐Qiong , Xiong Yan‐Ju , Cheng Qian , Zhu Fei‐Fei , Li Yun , Yue Shan‐Tang 
Published: October 2015

AbstractFour ZnII/CdII coordination polymers (CPs) based on 2‐(4‐carboxy‐phenyl)imidazo[4, 5‐f]‐1, 10‐phenanthroline (HNCP) and different derivatives of 5‐R‐1, 3‐benzenedicarboxylate (5‐R‐1, 3‐BDC) (R = NO2, H, OH), [Zn(HNCP)(5‐NO2‐1, 3‐BDC)]n (1), [Cd(HNCP)(5‐NO2‐1, 3‐BDC)]n (2), [Zn(HNCP)(1, 3‐BDC)(H2O)2]n (3), and {[Zn(HNCP)(5‐OH‐1, 3‐BDC)(H2O)·H2O}n (4) were synthesized under hydrothermal conditions. Compounds 1–4 were determined by elemental analyses, IR spectroscopy, and single‐crystal and powder X‐ray diffraction. Compounds 1 and 2 are isomorphous, presenting a 4‐connected uninodal (4, 4)‐sql 2D framework with threefold interpenetration, which are further extended into the three‐dimensional (3D) supramolecular architecture through π···π stacking interactions between the aryl rings of 5‐NO2‐1, 3‐BDC. Compared to compound 1, 3 is obtained by using different reaction temperatures and metal‐ligand ratios, generating a 3D framework with –ABAB– fashion via π···π stacking interactions. Compound 4 is a 1D chain, which is further extended into a 3D supramolecular net by hydrogen bonds and π···π stacking interactions. The thermogravimetric and fluorescence properties of 1–4 were also explored.