bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abscisic acid deficiency increases defence responses against Myzus persicae in Arabidopsis
Authors: Hillwig Melissa S. , Chiozza Mariana , Casteel Clare L. , Lau Siau Ting , Hohenstein Jessica , Hernández Enrique , Jander Georg , MacIntosh Gustavo C. 
Published: May 2015

SummaryComparison of Arabidopsis thaliana (Arabidopsis) gene expression induced by Myzus persicae (green peach aphid) feeding, aphid saliva infiltration and abscisic acid (ABA) treatment showed a significant positive correlation. In particular, ABA‐regulated genes are over‐represented among genes that are induced by M. persicae saliva infiltration into Arabidopsis leaves. This suggests that the induction of ABA‐related gene expression could be an important component of the Arabidopsis–aphid interaction. Consistent with this hypothesis, M. persicae populations induced ABA production in wild‐type plants. Furthermore, aphid populations were smaller on Arabidopsis aba1‐1 mutants, which cannot synthesize ABA, and showed a significant preference for wild‐type plants compared with the mutant. Total free amino acids, which play an important role in aphid nutrition, were not altered in the aba1‐1 mutant line, but the levels of isoleucine (Ile) and tryptophan (Trp) were differentially affected by aphids in wild‐type and mutant plants. Recently, indole glucosinolates have been shown to promote aphid resistance in Arabidopsis. In this study, 4‐methoxyindol‐3‐ylmethylglucosinolate was more abundant in the aba1‐1 mutant than in wild‐type Arabidopsis, suggesting that the induction of ABA signals that decrease the accumulation of defence compounds may be beneficial for aphids.
Variation in the use of surveillance endomyocardial biopsy among pediatric heart transplant centers over time
Authors: Godown Justin , Harris Michelle T. , Burger Judith , Dodd Debra A. 
Published: May 2015

AbstractEMB is widely utilized for graft surveillance after HTx; however, there is significant variation in the frequency of surveillance EMB use during the first year post‐HTx. The aim of this study was to assess changes in the utilization of surveillance EMB over time among member institutions of PHTS. A survey of PHTS centers assessing the frequency of surveillance EMB use during the first year post‐HTx was conducted in 2006. The same survey was repeated in 2014 to assess changes in practice over time. The number of EMB in infants ranged from 0 to 9 and in adolescents 0 to 16. The number of EMB decreased or remained unchanged in the majority of centers. Fewer EMB are performed in infants compared to adolescents and this practice did not change over time. There was a significant decrease in surveillance EMB use in adolescents (p = 0.012). International centers perform significantly fewer EMB in adolescents when compared to centers within the United States (p = 0.006). There continues to be significant variation in the utilization of surveillance EMB, with a shift toward less reliance on EMB for adolescents in the current era. Further research is necessary to determine the optimal frequency of invasive monitoring that reduces costs without compromising outcomes.
Origins of the Intermediate Spectral Form in M100 Mutants of Photoactive Yellow Protein
Authors: Kumar Anil , Woolley George Andrew 
Published: May 2015

AbstractNumerous single‐site mutants of photoactive yellow protein (PYP) from Halorhodospira halophila and as well as PYP homologs from other species exhibit a shoulder on the short wavelength side of the absorbance maximum in their dark‐adapted states. The structural basis for the occurrence of this shoulder, called the “intermediate spectral form,” has only been investigated in detail for the Y42F mutation. Here we explore the structural basis for occurrence of the intermediate spectral form in a M121E derivative of a circularly permuted H. halophila PYP (M121E‐cPYP). The M121 site in M121E‐cPYP corresponds to the M100 site in wild‐type H. halophila PYP. High‐resolution NMR measurements with a salt‐tolerant cryoprobe enabled identification of those residues directly affected by increasing concentrations of ammonium chloride, a salt that greatly enhances the fraction of the intermediate spectra form. Residues in the surface loop containing the M121E (M100E) mutation were found to be affected by ammonium chloride as well as a discrete set of residues that link this surface loop to the buried hydroxyl group of the chromophore via a hydrogen bond network. Localized changes in the conformational dynamics of a surface loop can thereby produce structural rearrangements near the buried hydroxyl group chromophore while leaving the large majority of residues in the protein unaffected.
Buddhists and Violence: Historical Continuity/Academic Incongruities
Authors: Jerryson Michael 
Published: May 2015

AbstractWithin the last several decades, there has been a surge in Buddhist‐influenced conflicts in places like the Tibetan region of China, Myanmar, Thailand and Sri Lanka. Every religious system has had violent instances. In many ways, the phenomenon speaks more about the nature of humanity than it does about religion. People have violent tendencies making violence present in all religious traditions. One prominent challenge in the study of religion and violence is defining the parameters for violence. As perpetrators of violence rarely locate themselves as aggressors or see themselves as deficient in moral justifications, this essay treats violence as the act of harming oneself and/or others. However, it also outlines the ways in which Buddhists see intentionality as influential in the ethics of such acts. Although scholars of religion and violence have critically shown how religiously‐motivated violence is multidimensional and a multi‐determined phenomenon (e.g., Aho ; Juergensmeyer ; Jones ), there persists global patterns to align religious traditions like Islam as a cause for violence and others like Buddhism as an explanation of peace. Such wide‐sweeping comparisons are at best superficial assessments that draw upon limited evidence. There may be a rash of contemporary examples of Buddhist‐inspired violence in places like Sri Lanka, Myanmar, Thailand, and China, but this does not make Buddhism any more violent than other religions. However, incidents like recent Buddhist attacks in Sri Lanka, Myanmar and Thailand are important pieces to the puzzle of Buddhism and violence. On the most basic of levels, they dispel the ahistorical myth that Buddhists are wholly peaceful.
First Arrival and Collective Land Ownership: How Children Reason About Who Owns the Land
Authors: Verkuyten Maykel , Sierksma Jellie , Martinovic Borja 
Published: May 2015

AbstractFour survey experiments provide evidence that children (9–12 years) infer collective land ownership from first arrival. In Experiments 1 and 2, children indicated that a group owns an island relatively more than another group when having been or living on the island first. In the third experiment, it was found that first comers were considered to own the land more independently of whether the second group joined or succeeded them in living on the island. In Experiment 4, the first arrival principle to infer collective ownership was independent of the duration of stay of the first comers before being joined by the second group. Taken together, the findings provide clear evidence of the importance of first arrival for inferring collective place ownership.
Cyclin‐dependent kinase‐activating kinases CDKD;1 and CDKD;3 are essential for preserving mitotic activity in Arabidopsis thaliana
Authors: Takatsuka Hirotomo , Umeda‐Hara Chikage , Umeda Masaaki 
Published: May 2015

SummaryFor the full activation of cyclin‐dependent kinases (CDKs), not only cyclin binding but also CDK phosphorylation is required. This activating phosphorylation is mediated by CDK‐activating kinases (CAKs). Arabidopsis has four genes showing similarity to vertebrate‐type CAKs, three CDKDs (CDKD;1‐CDKD;3) and one CDKF (CDKF;1). We previously found that the cdkf;1 mutant is defective in post‐embryonic development, even though the kinase activities of core CDKs remain unchanged relative to the wild type. This raised a question about the involvement of CDKDs in CDK activation in planta. Here we report that the cdkd;1 cdkd;3 double mutant showed gametophytic lethality. Most cdkd;1‐1 cdkd;3‐1 pollen grains were defective in pollen mitosis I and II, producing one‐cell or two‐cell pollen grains that lacked fertilization ability. We also found that the double knock‐out of CDKD;1 and CDKD;3 caused arrest and/or delay in the progression of female gametogenesis at multiple steps. Our genetic analyses revealed that the functions of CDKF;1 and CDKD;1 or CDKD;3 do not overlap, either during gametophyte and embryo development or in post‐embryonic development. Consistent with these analyses, CDKF;1 expression in the cdkd;1‐1 cdkd;3‐1 mutant could not rescue the gametophytic lethality. These results suggest that, in Arabidopsis, CDKD;1 and CDKD;3 function as CAKs controlling mitosis, whereas CDKF;1 plays a distinct role, mainly in post‐embryonic development. We propose that CDKD;1 and CDKD;3 phosphorylate and activate all core CDKs, CDKA, CDKB1 and CDKB2, thereby governing cell cycle progression throughout plant development.
Increased Athletic Performance in Lighter Basketball Shoes: Shoe or Psychology Effect?
Authors: Mohr Maurice , Trudeau Matthieu B. , Nigg Sandro R. , Nigg Benno M. 
Published: May 2015

Purpose:To determine the effect of shoe mass on performance in basketball-specific movements and how this affects changes if an athlete is aware or not of the shoe’s mass relative to other shoes.Methods:In an experimental design, 22 male participants were assigned to 2 groups. In the “aware” group, differences in the mass of the shoes were disclosed, while participants in the other group were blinded to the mass of shoes. For both groups lateral shuffle-cut and vertical-jump performances were quantified in 3 different basketball-shoe conditions (light, 352 ± 18.4 g; medium, 510 ± 17 g; heavy, 637 ± 17.7 g). A mixed ANOVA compared mean shuffle-cut and vertical-jump performances across shoes and groups. For blinded participants, perceived shoeweight ratings were collected and compared across shoe conditions using a Friedman 2-way ANOVA.Results:In the aware group, performance in the light shoes was significantly increased by 2% (vertical jump 2%, P < .001; shuffle cut 2.1%, P < .001) compared with the heavy shoes. In the blind group, participants were unable to perceive the shoe-weight variation between conditions, and there were no significant differences in vertical-jump and shuffle-cut performance across shoes.Conclusions:Differences in performance of the aware participants were most likely due to psychological effects such as positive and negative expectancies toward the light and heavy shoes, respectively. These results underline the importance for coaches and shoe manufacturers to communicate the performance-enhancing benefits of products or other interventions to athletes to optimize their performance outcome.
Analysis of Physical Collisions in Elite National Rugby League Match Play
Authors: Cummins Cloe , Orr Rhonda 
Published: May 2015

Objective:
To investigate the impact forces of collision events during both attack and defense in elite rugby league match play and to compare the collision profiles between playing positions.

Participants:
26 elite rugby league players.

Methods:
Player collisions were recorded using an integrated accelerometer in global positioning system units (SPI-Pro X, GPSports). Impact forces of collisions in attack (hit-ups) and defense (tackles) were analyzed from 359 files from outside backs (n = 78), adjustables (n = 97), wide-running forwards (n = 136), and hit-up forwards (n = 48) over 1 National Rugby League season.

Results:
Hit-up forwards were involved in 0.8 collisions/min, significantly more than all other positional groups (wide-running forwards P = .050, adjustables P = .042, and outside backs P = .000). Outside backs experienced 25% fewer collisions per minute than hit-up forwards. Hit-up forwards experienced a collision within the 2 highest classifications of force (≥10 g) every 2.5 min of match play compared with 1 every 5 and 9 min for adjustables and outside backs, respectively. Hit-up forwards performed 0.5 tackles per minute of match play, 5 times that of outside backs (ES = 1.90; 95% CI [0.26,3.16]), and 0.2 hit-ups per minute of match play, twice as many as adjustables.

Conclusions:
During a rugby league match, players are exposed to a significant number of collision events. Positional differences exist, with hit-up and wide-running forwards experiencing greater collision events than adjustables and outside backs. Although these results may be unique to the individual team’s defensive- and attacking-play strategies, they are indicative of the significant collision profiles in professional rugby league.
Sleep, Travel, and Recovery Responses of National Footballers During and After Long-Haul International Air Travel
Authors: Fullagar Hugh H.K. , Duffield Rob , Skorski Sabrina , White David , Bloomfield Jonathan , Kölling Sarah , Meyer Tim 
Published: May 2015

Purpose:The current study examined the sleep, travel, and recovery responses of elite footballers during and after long-haul international air travel, with a further description of these responses over the ensuing competitive tour (including 2 matches).Methods:In an observational design, 15 elite male football players undertook 18 h of predominantly westward international air travel from the United Kingdom to South America (–4-h time-zone shift) for a 10-d tour. Objective sleep parameters, external and internal training loads, subjective player match performance, technical match data, and perceptual jet-lag and recovery measures were collected.Results:Significant differences were evident between outbound travel and recovery night 1 (night of arrival; P < .001) for sleep duration. Sleep efficiency was also significantly reduced during outbound travel compared with recovery nights 1 (P = .001) and 2 (P = .004). Furthermore, both match nights (5 and 10), showed significantly less sleep than nonmatch nights 2 to 4 and 7 to 9 (all P < .001). No significant differences were evident between baseline and any time point for all perceptual measures of jet-lag and recovery (P > .05), although large effects were evident for jet-lag on d 2 (2 d after arrival).Conclusions:Sleep duration is truncated during long-haul international travel with a 4-h time-zone delay and after night matches in elite footballers. However, this lost sleep appeared to have a limited effect on perceptual recovery, which may be explained by a westbound flight and a relatively small change in time zones, in addition to the significant increase in sleep duration on the night of arrival after the long-haul flight.
The Effect of Glenohumeral Internal-Rotation Deficit on Functional Rotator-Strength Ratio in Adolescent Overhead Athletes
Authors: Guney Hande , Harput Gulcan , Colakoglu Filiz , Baltaci Gul 
Published: May 2015

Context:Glenohumeral (GH) internal-rotation deficit (GIRD) and lower eccentric external-rotator (ER) to concentric internal-rotator (IR) strength (ER:IR) ratio have been documented as risk factors for shoulder injuries, but there is no information on whether GIRD has an adverse effect on ER:IR ratio in adolescent overhead athletes.Objectives:The aim of this study was to investigate the effects of GIRD on functional ER:IR ratio of the adolescent overhead athletes.Design:Cross-sectional study.Setting:University research laboratory.Participants:52 adolescent overhead athletes.Main Outcome Measures:To determine GIRD, the range of GH IR and ER motion was measured with a digital inclinometer. An isokinetic dynamometer was used to assess eccentric and concentric IR and ER muscle strength of the dominant and nondominant shoulders. One-way ANCOVA where sport type was set as a covariate was used to analyze the difference between athletes with and without GIRD.Results:After standardized examinations of all shoulders, the athletes were divided into 2 groups, shoulders with (n = 27) and without GIRD (n = 25). There was a significant difference between groups in functional ER:IR ratio (P < .001). Athletes with GIRD had lower ER:IR ratio (0.56) than athletes without GIRD (0.83).Conclusions:As GIRD has an adverse effect on functional ratio of the shoulder-rotator muscles, interventions for adolescent overhead athletes should include improving GH-rotation range of motion.
Validity and Reliability of 2 Goniometric Mobile Apps: Device, Application, and Examiner Factors
Authors: Wellmon Robert H. , Gulick Dawn T. , Paterson Mark L. , Gulick Colleen N. 
Published: May 2015

Context:Smartphones are being used in a variety of practice settings to measure joint range of motion (ROM). A number of factors can affect the validity of the measurements generated. However, there are no studies examining smartphone-based goniometer applications focusing on measurement variability and error arising from the electromechanical properties of the device being used.Objective:To examine the concurrent validity and interrater reliability of 2 goniometric mobile applications (Goniometer Records, Goniometer Pro), an inclinometer, and a universal goniometer (UG).Design:Nonexperimental, descriptive validation study.Setting:University laboratory.Participants:3 physical therapists having an average of 25 y of experience.Main Outcome Measures:Three standardized angles (acute, right, obtuse) were constructed to replicate the movement of a hinge joint in the human body. Angular changes were measured and compared across 3 raters who used 3 different devices (UG, inclinometer, and 2 goniometric apps installed on 3 different smartphones: Apple iPhone 5, LG Android, and Samsung SIII Android). Intraclass correlation coefficients (ICCs) and Bland-Altman plots were used to examine interrater reliability and concurrent validity.Results:Interrater reliability for each of the smartphone apps, inclinometer and UG were excellent (ICC = .995–1.000). Concurrent validity was also good (ICC = .998–.999). Based on the Bland-Altman plots, the means of the differences between the devices were low (range = –0.4° to 1.2°).Conclusions:This study identifies the error inherent in measurement that is independent of patient factors and due to the smartphone, the installed apps, and examiner skill. Less than 2° of measurement variability was attributable to those factors alone. The data suggest that 3 smartphones with the 2 installed apps are a viable substitute for using a UG or an inclinometer when measuring angular changes that typically occur when examining ROM and demonstrate the capacity of multiple examiners to accurately use smartphone-based goniometers.
Genetic Diversity in the Collaborative Cross Model Recapitulates Human West Nile Virus Disease Outcomes
Authors: Graham Jessica B. , Thomas Sunil , Swarts Jessica , McMillan Aimee A. , Ferris Martin T. , Suthar Mehul S. , Treuting Piper M. , Ireton Renee , Gale Michael , Lund Jennifer M. 
Published: May 2015

ABSTRACT

West Nile virus (WNV) is an emerging neuroinvasive flavivirus that now causes significant morbidity and mortality worldwide. The innate and adaptive immune responses to WNV infection have been well studied in C57BL/6J inbred mice, but this model lacks the variations in susceptibility, immunity, and outcome to WNV infection that are observed in humans, thus limiting its usefulness to understand the mechanisms of WNV infection and immunity dynamics. To build a model of WNV infection that captures human infection outcomes, we have used the Collaborative Cross (CC) mouse model. We show that this model, which recapitulates the genetic diversity of the human population, demonstrates diversity in susceptibility and outcomes of WNV infection observed in humans. Using multiple F1 crosses of CC mice, we identified a wide range of susceptibilities to infection, as demonstrated through differences in survival, clinical disease score, viral titer, and innate and adaptive immune responses in both peripheral tissues and the central nervous system. Additionally, we examined the
Oas1b
alleles in the CC mice and confirmed the previous finding that
Oas1b
plays a role in susceptibility to WNV; however, even within a given
Oas1b
allele status, we identified a wide range of strain-specific WNV-associated phenotypes. These results confirmed that the CC model is effective for identifying a repertoire of host genes involved in WNV resistance and susceptibility. The CC effectively models a wide range of WNV clinical, virologic, and immune phenotypes, thus overcoming the limitations of the traditional C57BL/6J model, allowing genetic and mechanistic studies of WNV infection and immunity in differently susceptible populations.

IMPORTANCE
Mouse models of West Nile virus infection have revealed important details regarding the innate and adaptive immune responses to this emerging viral infection. However, traditional mouse models lack the genetic diversity present in human populations and therefore limit our ability to study various disease outcomes and immunologic mechanisms subsequent to West Nile virus infection. In this study, we used the Collaborative Cross mouse model to more effectively model the wide range of clinical, virologic, and immune phenotypes present upon West Nile virus infection in humans.
Ionoacoustics: A new direct method for range verification
Authors: Parodi Katia , Assmann Walter 
Published: May 2015

The superior ballistic properties of ion beams may offer improved tumor-dose conformality and unprecedented sparing of organs at risk in comparison to other radiation modalities in external radiotherapy. However, these advantages come at the expense of increased sensitivity to uncertainties in the actual treatment delivery, resulting from inaccuracies of patient positioning, physiological motion and uncertainties in the knowledge of the ion range in living tissue. In particular, the dosimetric selectivity of ion beams depends on the longitudinal location of the Bragg peak, making in vivo knowledge of the actual beam range the greatest challenge to full clinical exploitation of ion therapy. Nowadays, in vivo range verification techniques, which are already, or close to, being investigated in the clinical practice, rely on the detection of the secondary annihilation photons or prompt gammas, resulting from nuclear interaction of the primary ion beam with the irradiated tissue. Despite the initial promising results, these methods utilize a not straightforward correlation between nuclear and electromagnetic processes, and typically require massive and costly instrumentation. On the contrary, the long-term known, yet only recently revisited process of "ionoacoustics", which is generated by local tissue heating especially at the Bragg peak, may offer a more direct approach to in vivo range verification, as reviewed here.