bluuebunny/crossref_metadata_2025_split huggingface dataset:
17β‐estradiol promotes the progression of temporomandibular joint osteoarthritis by regulating the FTO/IGF2BP1/m6A‐NLRC5 axis
Authors: Xue Xintong , Li Changyi , Chen Shuang , Zheng Yan , Zhang Fan , Xu Yan 
Published: August 2024

AbstractBackgroundTemporomandibular joint osteoarthritis (TMJOA) is a degenerative cartilage disease. 17β‐estradiol (E2) aggravates the pathological process of TMJOA; however, the mechanisms of its action have not been elucidated. Thus, we investigate the influence of E2 on the cellular biological behaviors of synoviocytes and the molecular mechanisms.MethodsPrimary fibroblast‐like synoviocytes (FLSs) isolated from rats were treated with TNF‐α to establish cell model, and phenotypes were evaluated using cell counting kit‐8, EdU, Tanswell, enzyme‐linked immunosorbent assay, and quantitative real‐time PCR (qPCR). The underlying mechanism of E2, FTO‐mediated NLRC5 m6A methylation, was assessed using microarray, methylated RNA immunoprecipitation, qPCR, and western blot. Moreover, TMJOA‐like rat model was established by intra‐articular injection of monosodium iodoacetate (MIA), and bone morphology and pathology were assessed using micro‐CT and H&E staining.ResultsThe results illustrated that E2 facilitated the proliferation, migration, invasion, and inflammation of TNF‐α‐treated FLSs. FTO expression was downregulated in TMJOA and was reduced by E2 in FLSs. Knockdown of FTO promoted m6A methylation of NLRC5 and enhanced NLRC5 stability by IGF2BP1 recognition. Moreover, E2 promoted TMJ pathology and condyle remodeling, and increased bone mineral density and trabecular bone volume fraction, which was rescued by NLRC5 knockdown.ConclusionE2 promoted the progression of TMJOA.
Physiologically Based Pharmacokinetic Modeling of Vancomycin in Critically Ill Neonates: Assessing the Impact of Pathophysiological Changes
Authors: Shuai Weiwei , Cao Jing , Qian Miao , Tang Zhe 
Published: August 2024

AbstractDosing vancomycin for critically ill neonates is challenging owing to substantial alterations in pharmacokinetics (PKs) caused by variability in physiology, disease, and clinical interventions. Therefore, an adequate PK model is needed to characterize these pathophysiological changes. The intent of this study was to develop a physiologically based pharmacokinetic (PBPK) model that reflects vancomycin PK and pathophysiological changes in neonates under intensive care. PK‐sim software was used for PBPK modeling. An adult model (model 0) was established and verified using PK profiles from previous studies. A neonatal model (model 1) was then extrapolated from model 0 by scaling age‐dependent parameters. Another neonatal model (model 2) was developed based not only on scaled age‐dependent parameters but also on quantitative information on pathophysiological changes obtained via a comprehensive literature search. The predictive performances of models 1 and 2 were evaluated using a retrospectively collected dataset from neonates under intensive care (chictr.org.cn, ChiCTR1900027919), comprising 65 neonates and 92 vancomycin serum concentrations. Integrating literature‐based parameter changes related to hypoalbuminemia, small‐for‐gestational‐age, and co‐medication, model 2 offered more optimized precision than model 1, as shown by a decrease in the overall mean absolute percentage error (50.6% for model 1; 37.8% for model 2). In conclusion, incorporating literature‐based pathophysiological changes effectively improved PBPK modeling for critically ill neonates. Furthermore, this model allows for dosing optimization before serum concentration measurements can be obtained in clinical practice.
Increased levels of circulating cell‐free double‐stranded nucleic acids in the plasma of glioblastoma patients
Authors: Rackles Elisabeth , Zaccheroni Elena , Lopez Patricia Hernandez , Faletti Stefania , Bene Massimiliano Del , DiMeco Francesco , Pelicci Giuliana , Falcon‐Perez Juan M 
Published: August 2024

AbstractCirculating cell‐free nucleic acids are considered a promising source of biomarkers for diseases and cancer. Liquid biopsy biomarkers for brain tumours represent a major, still unmet, clinical need. In plasma, nucleic acids can be free or be associated with extracellular vesicles (EVs). Here we report an easy and reproducible method to analyse cell‐free nucleic acids in plasma and EVs by conventional flow cytometry easy to translate into the clinics. Nucleic acids associated with the EVs or present in plasma samples are stained by Pyronin Y, which is a fluorescent dye that is preferably binding double‐stranded nucleic acids. Fluorescent staining of EVs isolated from cell‐conditioned media is suitable for DNA and RNA detection by flow cytometry. The nucleic acids are partially protected from degradation by the EVs’ membrane. Additionally, DNA and RNA can be stained in plasma samples and plasma‐derived EVs. Remarkably, analysis of plasma from patients and healthy individuals reveals a difference in their nucleic acid profiles. Taken together, our results indicate that the proposed methodology, which is based on conventional direct flow cytometry, is a promising easy tool for plasma nucleic acid analysis.
A matter of timing? A systematic review of work scheduling dynamics in work recovery research and applications
Authors: Sloan Molly M. , Busick Cortney , Mitropoulos Tanya , Dosumu Fiyinfunjah , Calderwood Charles 
Published: August 2024

SummaryWork recovery reflects the replenishment of personal resources depleted by working, which has implications for employee health and wellness. However, work scheduling factors have received very limited attention in the recovery literature, despite that recovery is a dynamic process widely recognized to be influenced by contextual factors that define and influence the work role. After first conducting a narrative review of whether and how work scheduling factors are accounted for in existing theories of work recovery, we conduct a systematic review of existing work recovery research to identify any past empirical consideration of work scheduling factors in the recovery research base. We then harness the results of this systematic review to develop a taxonomy of work scheduling and related contextual factors that may be relevant to the process of recovery from work. We discuss the theoretical, practical, and methodological implications that emerged from our narrative and systematic reviews, providing guidance for how this newly developed taxonomy can be applied to understanding the implications of scheduling dynamics for work recovery across a range of different work contexts.
Pioneering Nit Gene Exploitation to Develop Molecular Diagnostic Assay for Rapid Detection of Cotton Root Rot Incitant, Macrophomina phaseolina (Tassi) Goid, in Field Soil
Authors: Saini Anil Kumar , Kumar Mukesh , Singh Karmal , Bhambhu Mukul Kumar , Nain Rohit , Garima  , Aakash  , Mandhania Shiwani , Saini Shubham 
Published: August 2024

ABSTRACTCotton root rot caused by Macrophomina phaseolina pose a significant threat to cotton production, leading to substantial yield and quality losses. Early and accurate diagnosis of this pathogen in soil is crucial for effective disease management. This study presents a pioneering investigation into the utilization of the nit gene encoding nitrilase for the development of a molecular diagnostic assay aimed at the rapid detection of M. phaseolina in field soils. The methodology involved the design and validation of primers targeting the Nit gene sequence, followed by the optimization of PCR conditions for efficient amplification. Leveraging state‐of‐the‐art molecular techniques, the assay offers a novel protocol to accurately identify the presence of M. phaseolina in soil with high sensitivity and specificity. The specificity of the designed primers was confirmed through PCR amplification using DNA from M. phaseolina and other related fungi. Sensitivity tests demonstrated that the PCR assay reliably detected M. phaseolina DNA at concentrations as low as 1 ng. Furthermore, the performance of the diagnostic assay was rigorously evaluated using field soil samples with a known status of M. phaseolina infection, demonstrating its reliability and efficacy in real‐world scenarios. This study introduces a novel molecular marker for the detection of M. phaseolina and offers a rapid and efficient means for screening M. phaseolina in large soil samples with minimal time and manpower.
Seasonally dry tropical forests in the late Pleistocene of Mesopotamia, Argentina and their relationship to environmental changes during the Last Interglacial
Authors: Fernandez Pacella L. , Baez J. , Crisafulli A. , Roig M. , Di Pasquo M. , Luna C.A. , Fagúndez I. , Martinez M. , Cuaranta P. 
Published: August 2024

ABSTRACTThe concept of seasonal forests groups structural types of vegetation that are related to climatic seasonality in the tropics of South America. Consequently, this determines the physiognomy of the vegetation, from semi‐deciduous to strongly deciduous. The strongest link between seasonally dry tropical forests (SDTFs) is their floristic composition, where Leguminosae and Anacardiaceae dominate the woody flora. The fossil records of the Neogene of northwestern Argentina reveal a list of species found in various locations and formations of the Miocene–Lower Pleistocene obtained from studies of pollen, woods (logs), cuticles, impressions of leaves and fruits. The analysis of sediments and woody structures from the Tapebicuá, Toropí/Yupoí and El Palmar Formations (Upper Pleistocene) of the Mesopotamia region allowed us to identify several pollen taxa and silicified wood fragments (mineralized). The woody and shrubby association whose current relatives characterize the SDTF is composed of the 16 fossil species described here belonging to seven families. In our samples, Anadenanthera colubrina and Myracrodruon balansae are the most significant members of the families Leguminosae and Anacardiaceae, which are dominant in the SDTF. The paleobotanical species described in this study confirm the extension of the SDTF to the province of Corrientes, coinciding with various climatic events (dry subtropical, semi‐desert and warm‐humid climate) that would have favored the development of these forests during the Pleistocene in this region. The absolute dates obtained for the Toropí/Yupoí and Tapebicuá Formations confirm their synchronicity and correlation to Marine Isotope Stage 5. The palynological analysis, the presence of Menendoxylon and the sedimentological data allow us to infer the existence of a seasonally dry humid paleoclimate in northeastern Argentina during the Late Pleistocene favorable to the development of the SDTF.
Effect of sorghum bran addition on antioxidant activities, sensory properties, and in vitro starch digestibility of Chinese southern‐style steamed bread
Authors: Zhang Pingping , Awika Joseph M 
Published: August 2024

AbstractBACKGROUNDChinese steamed bread (CSB) is one of the most important staple foods in China and is also popular in South‐East Asia. Developing functional CSB could improve people's resistance to inflammatory and non inflammatory diseases. This work investigated the effect of sorghum bran addition on antioxidant activities, sensory properties, and in vitro starch digestibility of Chinese southern‐style steamed bread (CSSB).RESULTSIn this study, the enhanced CSSB with 0–200 g kg−1 of fine black and tannin (sumac) sorghum bran addition was developed. A small change in phenol content and antioxidant activity was observed at various stages in the processing procedure before steaming. Moreover, a high retention of antioxidant phenolics CSSB with sorghum bran addition was observed. Sorghum bran addition significantly increased the total phenol content and antioxidant activity of CSSB by 4.5–10 times, on average, relative to control. Sorghum bran addition significantly also increased the content of resistant starch, and significantly decreased in vitro starch digestibility in CSSB; these effects were likely due to the joint inhibitory effect of tannins and ferulic acid on starch digestibility. Interestingly, the sorghum bran breads scored higher or similar to control in sensory color preference and overall appearance, but lower on most textural and mouthfeel attributes.CONCLUSIONSorghum bran addition significantly increased the antioxidant activity of CSSB and significantly decreased starch digestibility. Moreover, the color and appearance properties were maintained or improved. However, the sensorial textural attributes were negatively impacted by the sorghum bran substitutions. Strategies to improve the texture of bran‐fortified breads would likely enhance their consumer acceptability. © 2024 Society of Chemical Industry.
Unintended consequences of wildlife feeders on spatiotemporal activity of white‐tailed deer, coyotes, and wild pigs
Authors: Saldo Elizabeth A. , Jensen Alex J. , Muthersbaugh Michael S. , Ruth Charles , Cantrell Jay , Butfiloski Joseph W. , Yarrow Greg K. , Kilgo John C. , Jachowski David S. 
Published: August 2024

AbstractSupplemental feeding of wild ungulates has long been and remains a common practice across Europe and North America. Yet by drawing animals together, supplemental feeding can have unintended, negative effects on individual species and broader ecological processes. These include increased risk of disease transmission, intraspecific and interspecific competition, and predation, which are of management concern for white‐tailed deer (Odocoileus virginianus) in the southeastern United States given the arrival of nonnative wild pigs (Sus scrofa) and coyotes (Canis latrans). We conducted a field experiment between March and July of 2021 to assess the effects of supplemental feeding on spatiotemporal activity patterns of deer and wild pigs at wildlife feeders, and space use of coyotes in the Piedmont region of South Carolina, USA. We observed support for our hypothesis that interspecific competition through increased visitation by larger groups of competitor species reduces use of foraging sites by other subordinate ungulates, where feeders highly visited by wild pigs were rarely visited by deer. While adult deer and wild pigs generally did not shift their temporal activity patterns at feeders, juvenile temporal activity shifted to more frequent visits of feeders during the night, supporting our hypothesis that supplemental feed could increase risk to predator exposure, as coyotes tend to be active during crepuscular hours. Our findings suggest that supplemental feed put out to encourage deer activity could actually deter deer if wild pigs occupy that area, and has potential negative demographic effects if juveniles are at increased risk of predation. Collectively, based on our data, we do not recommend supplemental feeding in the southeastern United States where white‐tailed deer, coyotes, and wild pigs co‐occur. More broadly, given how widespread the legal use of supplemental feed remains across the United States, we encourage landowners and policymakers to consider the full suite of potential direct and indirect, short‐term and long‐term negative impacts supplemental feeding can have on both target and nontarget wildlife populations.
Surgical treatment for medium‐sized cystic osteochondral lesions of the talus: Autologous osteoperiosteal transplantation provides better clinical outcomes than bone marrow stimulation when cysts are deeper than 6 mm
Authors: Yang Shuai , Shao Qirui , Zhu Yu , Hu Fengyi , Jiang Dong , Jiao Chen , Hu Yuelin , Shi Weili , Guo Qinwei 
Published: August 2024

AbstractPurposeTo compare clinical outcomes of autologous osteoperiosteal transplantation versus bone marrow stimulation (BMS) for medium‐sized (100–150 mm2) cystic osteochondral lesions of the talus (OLTs) and assess the correlation between patient demographics and outcomes. It was hypothesised that autologous osteoperiosteal transplantation would provide better clinical outcomes than BMS for medium‐sized cystic OLTs.MethodsPatients who underwent autologous osteoperiosteal transplantation or BMS for medium‐sized cystic OLTs between 2014 and 2019 were retrospectively evaluated. According to their characteristics, a 1:1 propensity‐score matching was performed and 33 pairs of patients were matched. The visual analogue scale, American Orthopaedic Foot and Ankle Society (AOFAS) score, Foot Ankle Outcome Score (FAOS) and Ankle Activity Score were collected preoperatively and at the last follow‐up. In addition, a general linear model analysis was performed between patient demographics and clinical outcomes in two groups separately to detect potential risk factors.ResultsFinally, 28 patients in the grafted group and 27 patients in the BMS group completed the follow‐up and were enrolled with a mean follow‐up period of 63.5 ± 13.9 months. Both groups showed significant improvement in all patient‐reported outcomes (p < 0.01). At the final follow‐up, no significant differences between groups were found in all postoperative scores except FAOS Pain (p = 0.02). Correlation analysis showed a moderate correlation between cyst depth and the postoperative AOFAS score in the BMS group (r = −0.48, p = 0.01). Based on the regression line, the patients in the BMS group with a cyst deeper than 6 mm showed a lower AOFAS score than the mean score (88.7 ± 9.5) of the grafted group.ConclusionAutologous osteoperiosteal transplantation and BMS are both safe and effective for medium‐sized cystic OLTs. However, autologous osteoperiosteal transplantation is expected to provide better clinical outcomes than BMS when the cysts are deeper than 6 mm.Level of EvidenceLevel III.
Gradual stabilization and narrowing of bone tunnels following primary anterior cruciate ligament reconstruction
Authors: Liu Di , Lu Wenhao , Vithran Djandan Tadum Arthur , Bi Qing , Hong Zheping , Liu Xu , Yuan Dongliang , Chen Can , Xiao Wenfeng , Li Yusheng 
Published: August 2024

AbstractPurposeThe purpose of this study is to dynamically assess variations in tunnel diameters following anterior cruciate ligament reconstruction (ACLR) and investigate correlations with patient‐reported outcomes (PROs) and graft maturity based on signal‐to‐noise quotient (SNQ).MethodsTunnel diameter and tunnel position were measured using three‐dimensional models derived from computed tomography (CT) data. Postoperative graft maturity and integration were evaluated using magnetic resonance imaging (MRI). Clinical outcomes were assessed through PROs, which included the International Knee Documentation Committee Subjective Knee Evaluation Form, Knee Injury and Osteoarthritis Outcome Scores and Lysholm scores. The correlation between tunnel enlargement extent, PROs and SNQ values, as well as correlations between confounding factors, tunnel diameter differences and SNQ were analyzed.ResultsA total of 73 participants underwent primary ACLR and scheduled follow‐ups. At the segment of the articular aperture, the femoral tunnel was enlarged by 32.3% to 10.4 ± 1.6 mm (p < 0.05), and the tibial tunnel was widened by 17.2% to 9.6 ± 1.2 mm (p < 0.05) at the 6‐month follow‐up. At 1 year postoperatively, diameters at the articular aperture were not further increased on the femoral (n.s.) and tibial (n.s.) sides. In early postoperative follow‐up, the femoral tunnel was anteriorly and distally shifted, coupled with posterior and lateral deviation involving the tibial side, exhibiting minimal migration at 1‐year follow‐up. The degree of tunnel widening was not correlated with PROs and SNQ values. Age, gender, body mass index (BMI), time from surgery to follow‐up, concomitant injuries and autograft type were not correlated with tunnel diameter differences and SNQ.ConclusionsThe femoral and tibial bone tunnels exhibited eccentrical widening and gradually stabilized at 1 year following ACLR. Furthermore, the enlarged bone tunnels were not correlated with unsatisfied PROs and inferior graft maturity.Level of evidenceLevel IV.
Recruitment responses of shade‐tolerant and heliophilous trees in degraded areas: The necessity of knowing the recruitment autecology of species for effective reforestation decisions
Authors: Croce Johanna , Badano Ernesto I. , Tálamo Andrés 
Published: August 2024

AbstractDirect seeding is a reforestation technique that allows introducing a great variety of tree species in degraded areas with less logistics, but tree establishment rates are usually low because developing plants do not tolerate the high sun exposure, extreme temperatures, and low soil moisture that prevail in these habitats. This leads to the proposal that seeds should be planted beneath shrubs or herbaceous plants that ameliorate harsh abiotic conditions, although this can also trigger interspecific competition and impair the performance of developing trees. This study aimed to test these proposals in dry forests in northwest Argentina. For this, seeds of two shade‐tolerant trees and a heliophilous tree, native to this region, were sowed beneath the canopy of pioneer shrubs and in open spaces with and without herbaceous plants. After 2 years, our findings indicated that shade‐tolerant trees performed better beneath shrub canopies, while the heliophilous tree achieved higher establishment rates in the open spaces. Nevertheless, it seems that herbaceous plants compete with the tree seedlings and, therefore, they should be removed before conducting reforestation programs. Thus, we propose that direct seeding could be an efficient reforestation strategy, but the regeneration autecology of the tree species selected for this task, as well as their competitive ability in the face of the pioneer vegetation, must be carefully evaluated before seeding.
Soil quality associated with microbial community characteristics and dominant taxa across different tillage practices
Authors: Liu Wen‐Sheng , Zhu Zhang‐Hua , Lu Zhi‐Gang , Oladele Olatunde Pelumi , Dang Yash Pal , Zhao Xin , Zhang Hai‐Lin 
Published: August 2024

AbstractDespite conservation tillage being a promising strategy to mitigate soil degradation, the intricate role of microbial communities in shaping soil quality over long‐term tillage remains poorly understood. The study aimed to investigate the microbial mechanisms governing the soil quality index (SQI) and maize yield under different tillage practices spanning 13 years, including no‐till without straw retention (NT0), no‐till with straw retention (NTSR), plough tillage with straw retention (PTSR), and rotary tillage with straw retention (RTSR). The findings revealed that NTSR improved the SQI index by 22.4% and 11.3% higher than PTSR and RTSR, respectively, within the 0–10 cm soil layer. This improvement was correlated with an increase in maize yield (R2 = 0.39, p < 0.05). PERMANOVA analysis confirmed that both soil depth and tillage practices significantly impacted the composition of microbial communities (p < 0.05). Furthermore, conservation tillage, compared to PTSR and RTSR, increased the abundance of arbuscular mycorrhizal symbiosis by 78.6%–460.3% but decreased the saprophytic fungal abundance by 27.5%–28.3%. Soil quality was notably influenced by the interaction between bacterial and fungal communities. The presence of bacterial‐dominated Module 2 was associated with decreased soil quality in the 0–10 cm soil depth (r = −0.47, p < 0.01). This study emphasizes the pivotal role of microbial diversity and dominant taxa in driving soil quality after long‐term conservation tillage practices. Understanding these mechanisms is crucial for establishing farmland management to achieve agricultural and ecological sustainability in the face of climate change and soil degradation challenges.