bluuebunny/crossref_metadata_2025_split huggingface dataset:
Giant Metastatic Malignant Melanoma with an Unknown Primary Site
Authors: Morita Kazumasa , Kudo Hitoshi , Fujii Kimio , Okamoto Hiroyuki , Matsubara Kunio , Kanauchi Hideo , Imamura Sadao 
Published: April 2015

AbstractWe report a case of malignant melanoma of unknown primary origin which presented with a giant metastatic tumor in his right inguinal region. A 94‐year‐old man noticed a small subcutaneous tumor in the right inguinal region 3 years earlier, which eventually became as large as 9 cm in diameter without treatment. Although a histological examination of the lesion showed malignant melanoma, extensive examination did not reveal its primary lesion or any metastasis other than that to the right inguinal area. Our case took an interesting course in that this well‐growing metastatic tumor was localized in only one region and supported a previous report indicating that malignant melanoma with unknown primary origin has a low tendency to metastasize and a relatively good prognosis.
Lichen Sclerosis et Atrophicus with Prominent Teleangiectasia
Authors: Kanekura Takuro , Kawamura Kyoko , Kanzaki Tamotsu 
Published: April 2015

AbstractLichen sclerosis et atrophicus is characterized by the development of well demarcated, depigmented macules with epidermal atrophy. Hemorrhage occurs occasionally; however, teleangiectasia is seldom remarkable.We studied a case of lichen sclerosis et atrophicus with striking teleangiectasia which involved a 62‐year‐old male who was suffering from this disorder on his upper back. An initial lesion had been excised 15 years earlier. Thirteen years after the original surgery, he noticed a similar skin eruption accompanied by teleangiectasia that had recurred around the surgical scar.We present here a detailed description of this case and provide a short review of the literature.
Comparative Study of Staphylococcal Flora on the Skin Surface of Atopic Dermatitis Patients and Healthy Subjects
Authors: Ogawa Tadatomo , Katsuoka Kensei , Kawano Kyoko , Nishiyama Shigeo 
Published: April 2015

AbstractBacterial samples were obtained from the facial and forearm skin of atopic dermatitis (AD) patients and healthy subjects in humid summer weather. Staphylococcus (Staph) species bacteria were selectively sampled by a plate‐contact technique using mannitol salt medium‐coated film. Staph species were isolated from the film after the number of colonies was counted, and the species were determined by the API‐Staph system and coagulase test. Large numbers of S. aureus were isolated in the samples from the facial skin of patients with severe dermatitis, as previously reported. The number of S. aureus colonies in the samples from the forearm skin of the patients was significantly greater than from the healthy subjects; the mean and standard deviation of colony forming units per 10 cm2, expressed as log10 value, were 1.64 ± 0.96 for patients and 0.02 ± 0.07 for healthy subjects (p<0.01). One hundred and seventeen strains were isolated, and their sensitivities to ten antibiotics were tested. Ten of the strains were resistant to some antibiotics; all of these were isolated from patients. Penicillin type antibiotics had weak effects, but cephem type had strong effects. The number of S. aureus colonies isolated in this study was compared with the number isolated during the winter, in our previous study, in order to identify seasonal variations. The only significant seasonal difference was on the forearm skin of the AD patients, which had significantly more S. aureus colonies (p<0.01) in this study. There were no significant seasonal differences in S. aureus numbers on the facial skin of patients or healthy subjects.
Accuracy in the Clinical Diagnosis and Pattern of Malignant Melanoma at a Dermatological Clinic
Authors: Lindelöf Bernt , Hedblad Mari‐Anne 
Published: April 2015

AbstractThe diagnostic pattern of malignant melanoma and clinical suspicion rate has been investigated at a dermatological university clinic. Of 174 histologically proven malignant melanomas, 60 (34%) were not clinically suspected as melanomas. The accuracy of clinical diagnosis increased with level of experience. Physicians with <1 year experience in dermatology were able to detect malignant melanoma as the first diagnosis in 31% of the cases in contrast to 63% for those with >10 years experience. Of 50 patients immediately referred from the dermatologic clinic to surgery departments, 12 did not have melanoma.
Pathogenesis of Mucocutaneous Lesions in Behçet's Disease
Authors: Inoue Chiyuki , Itoh Rie , Kawa Yoko , Mizoguchi Masako 
Published: April 2015

AbstractBehcet's disease (BD) is characterized by recurrent oral aphthae, skin lesions, eye lesions, and genital ulceration. To determine the pathogenesis of BD, we performed histological and immunohistochemical studies of these mucocutaneous lesions, an assay of neutrophil activity, and HLA typing.Dense dermal or subcutaneous infiltrations of polymorphonuclear cells (PMN) without leukocytoclastic vasculitis were found in 28 of 57 lesions. Immunohistochemically, deposits of C3 on the vessels were found in 12 of 31 lesions. Deposits of immunoglobulin were not found except for one of IgM. C3 deposits and PMN infiltrations were significantly related (p<0.05). PMN activity by polarization was enhanced; however, the results did not show a significant relationship with the PMN infiltrations or the C3 deposits. The incidence of HLA‐B51 was significantly high in BD, but no significant relationship was found between HLA‐B51 and the results of other examinations.These results suggest that the pathogenesis of BD lesions differs from that of collagen diseases and that C3 deposits on the vessels may play an important role in the development of mucocutaneous lesions where PMN have mainly infiltrated.
Systematized Epidermal Nevus with Profuse Papillomatous Flexural Lesions
Authors: D'Souza Mariette , Garg Bhagat Ram , Ratnakar Chiganti 
Published: April 2015

AbstractA case of systematized epidermal nevus with profuse papillomatous flexural lesions is reported for its striking morphologic features and unusual variations in histopathology at different sites.
Bilateral Periorbital Eccrine Hidrocystoma
Authors: Yasaka Nami , Iozumi Ken , Nashiro Kiyoko , Tsuchida Tetsuya , Seki Yoshihito , Furue Masutaka , Takayama Osami , Shimada Shinji , Tamaki Kunihiko 
Published: April 2015

AbstractWe saw four patients showing identical features as cystic lesions on the bilateral external canthi. Histological examination showed cystic cavities in the dermis. Histological and enzyme histochemical findings suggest that these cystic tumors are of eccrine origin. Thus we diagnosed these cytic tumors as eccrine hidrocystoma with characteristic clinical feature. The recognition of this feature would help to correctly diagnose these eccrine hidrocystoma.
Infantile Myofibromatosis: Report of Two Cases
Authors: Sonoda Tadashige , Itami Satoshi , Seguchi Syunnichiro , Kurata Sotaro , Takayasu Susumu 
Published: April 2015

AbstractTwo cases of solitary type infantile myofibromatosis (IM) were presented. Case 1 was a 6‐month‐old male infant with a firm, dark red, fixed tumor on his right knee. A computerized tomographic scan revealed that the tumor was attached to the underlying muscle. Case 2 was a 1‐month‐old male infant with a tumor on his right knee, similar to that in case 1. In neither case was there any clinical evidence of visceral involvement. Histological and immunohistochemical findings were similar. The lesions appeared histologically as non‐encapsulated nodules composed of whorled fascicles of spindle‐shaped cells and a vascular element with a hemangiopericytoma‐like appearance. The tumor cells were positively stained with PTAH. They were positive for α‐smooth muscle actin and vimentin, but negative for desmin. These findings support the myofibroblastic nature of IM. In case 2, the tumor regressed spontaneously at the age of 12 months. Unlike the multicentric form, spontaneous regression of the solitary form of IM has not previously been reported.
Subgaleal Angiomyolipoma
Authors: Tamura Atsushi , Ishikawa Osamu , Miyachi Yoshiki 
Published: April 2015

AbstractA case of angiomyolipoma found in the subgaleal lesion is reported. Lipoma appearing between the galea and periosteum of the frontal bone is called subgaleal or subfascial lipoma and usually consists histopathologically of mature fat cells. We experienced a case of so‐called subgaleal lipoma; however, the distinctive histologic features of our case were the proliferation of non‐striated muscle fibers and small vessels scattered throughout the mature fat cells, which were compatible with angiomyolipoma. Extrarenal angiomyolipoma, especially cutaneous angiomyolipoma, are very rarely mentioned in the literature. Our case is the second reported patient with angiomyolipoma in the subgaleal location.
Identification of a Novel cDNA Clone Encoding Protein Tyrosine Kinase in Murine Skin
Authors: Kawachi Yasuhiro , Nakauchi Hiromitsu , Otsuka Fujio 
Published: April 2015

AbstractTyrosine phosphorylation is widely recognized as playing important roles in cell differentiation, proliferation, and carcinogenesis. We have used the polymerase chain reaction (PCR) method to identify protein tyrosine kinases that are expressed in the skin. Mixed oligonucleotide probes were used to amplify and screen a neonatal murine skin cDNA pool for clones encoding amino acid contiguities whose conservation is characteristic of the protein tyrosine kinase family. When the PCR products were sequenced, 13 distinct clones were found, of which one is novel to date and has provisionally been named tks (for tyrosine kinase identified from skin). Sequence homology comparison showed that the tks gene is homologous to the src and fes/fps families. Northern blotting using PCR products of tks as a probe revealed that the mRNA of tks is detected ubiquitously and weakly in other tissues such as brain, lung, liver, thymus and kidney. This fact suggests that the tks gene is expressed in widely distributed cell types.
Growth‐promoting Effect of Bacterial Products from Clostridium perfringens on Human Keratinocytes
Authors: Takada Akiyoshi , Hashimoto Koji , Yoshikawa Kunihiko 
Published: April 2015

AbstractWound healing substance (WHS) from cultured Clostridium perfringens has been reported to be effective in the treatment of wounds. The effects of WHS, now named SNK‐863, on proliferation and differentiation of human keratinocytes were examined.The characteristics of WHS are as follows: 1) WHS stimulates human keratinocyte growth and DNA synthesis; 2) WHS and EGF show some additive effects on human keratinocyte growth; 3) WHS does not interfere with the binding of EGF to its receptor; 4) WHS does not counteract the growth inhibitory effects of TGF‐β or vitamin D3 on human keratinocytes; 5) WHS has no significant effect on human keratinocyte differentiation. These results indicate that the growth‐promoting effect of WHS on keratinocytes may contribute to the treatment of wound healing.
A New Approach to the Evaluation of Broad‐spectrum Sunscreens against Ultraviolet and Visible Light‐induced Delayed Tanning
Authors: Kawada Akira , Morimoto Koukichi , Hiruma Masataro , Noda Toshiaki , Ishibashi Akira 
Published: April 2015

AbstractProtection against UV and visible light‐induced delayed tanning (DT) and immediate tanning (IT) of two broad‐spectrum sunscreens with the same sun protection factor was investigated. Protection factors (PFs) against DT (24 h and 7 days) of the sunscreens differed significantly, but PFs against IT did not. This study indicates that using PF against 24 h DT is more appropriate for an evaluation of broad‐spectrum sunscreens against UVA and visible light than IT.
Sympathetic Ganglion Blockade for the Management of Hyperhidrosis
Authors: Kobayashi Kinuko , Omote Keiichi , Homma Eiji , Abe Tatsuhiko , Iitoyo Miyuki 
Published: April 2015

AbstractWe present three patients with severe primary hyperhidrosis, refractory to conservative medical treatment, who were successfully managed with sympathetic ganglion blockade with ethanol. We also summarize 10 patients with hyperhidrosis who underwent sympathetic ganglion blockade in the past 2 years. This closed percutaneous method offers the patients considerably less discomfort and less stress with minimal morbidity and has a efficacy similar to that of surgical sympathectomy, which has previously been the only effective and permanent therapy for severe primary hyperhidrosis. It is concluded that chemical sympathectomy is an effective and useful method for treating severe hyperhidrosis which has advantages over surgical sympathectomy.
Male Genital Herpes Complicated with Urethral Infection
Authors: Yoshida Masami , Hondo Ryo , Tezuka Tadashi , Hiruma Masataro 
Published: April 2015

AbstractA 26‐year‐old man had a primary infection or reinfection of genital herpes due to herpes simplex virus (HSV) type 2 complicated with herpetic urethritis. By analysis of the restriction endonuclease digestion pattern of the viral DNA, two isolates, one from the penile lesion and one from the urethra, were identified as the same strain.
The Effects of Sunscreens on UVB Erythema and Langerhans Cell Depression
Authors: Miyagi Tsuguna , Bhutto Abdul Manan , Nonaka Shigeo 
Published: April 2015

AbstractLangerhans cells (LCs) are epidermal antigen‐presenting cells capable of initiating a specific T lymphocyte‐mediated immune response. It is a well known fact that ultraviolet light B (UVB) suppresses LC number and function.In this study, we confirmed that the sunscreens CITY BLOCK, and TOTAL SUN SHIELD 28 (Clinique Laboratories Tokyo, Japan) protected the epidermis against the depletion of LC number. We also investigated whether or not sunscreens could provide LC protection from ultraviolet ray (UVR) damage other than the prevention of the decrease in the total number of cells.Our data showed that the LC population was depressed after irradiation by 100 mJ/cm2 or 10 mJ/cm2 of UVB, but recovered to within normal levels after 16 days. Both sunscreens provided protection against erythema and LC depression due to UVB irradiation. However, despite the fact that these sunscreens had completely suppressed UVB erythema, shrinkage of LC dendrites was seen. Apparently, sunscreens prevent UVB erythema, but do not protect against functional changes in LC due to UVB.Recently, it has been reported that sunscreens are less effective in protecting against systemic immunosuppression that against inflammation. The shrinkage of LC dendrites despite sunscreen application may help explain this discrepancy.
Diagnostic Approach to Autoimmune Blistering Diseases Using Immunoblotting with Chemiluminescence
Authors: Kitamura Tetsunori , Tamada Yasuhiko , Takama Hiromichi , Yokochi Kikuka , Nitta Yukiko , Ikeya Toshihiko 
Published: April 2015

AbstractAutoantibodies in sera from patients with autoimmune blistering diseases were detected by immunoblotting with chemiluminescence. This method provided the high sensitivity in the detection of autoantibodies against human epidermal extract. It was useful for detecting low titers of autoantibodies, and identifying small amounts of autoantigens in antigen extracts.
Ultrasonographic Demonstration of a Skin Metastasis of Adenocarcinoma
Authors: Ichioka Shigeru , Yamada Atsushi 
Published: April 2015

AbstractA case of skin metastasis of adenocarcinoma on the back of a 69‐year‐old female was reported. The ultrasound appearance of the tumor indicated a malignant lesion prior to the detection of a primary lung carcinoma.
Wegener's Granulomatosis Successfully Treated with Prednisolone and Potassium Iodide
Authors: Torinuki Wakio 
Published: April 2015

AbstractWegener's granulomatosis has traditionally been treated with steroids and cyclophosphamide. We used potassium iodide in conjunction with prednisolone to treat a patient with a limited form of this disease who had gangrenous rhinitis and skin involvement including necrotic papules and ulcerations. After three months of this therapy, the mucosal and cutaneous lesions had almost completely disappeared.
Detection of Human Papillomavirus Type 60 in Plantar Cysts and Verruca Plantaris by the In situ Hybridization Method Using Digoxigenin Labeled Probes
Authors: Kawase Masaaki , Honda Manko , Niimura Michihito 
Published: April 2015

AbstractBackgroundSince 1987, many cases have been reported in which human papillomavirus (HPV) could be associated with epidermoid cysts of the palms and soles. In 1989, the HPV found in an induced epidermoid cyst was cloned and named HPV 60.ObjectiveOur purpose was to examine the relationship of HPV 60 to plantar cysts.MethodsTen cases of plantar cysts and two cases of verruca plantaris containing single inclusion bodies were obtained by biopsy and examined by the in situ hybridization (ISH) method with a digoxigenin labeled HPV 60 DNA probe.ResultsSix of ten cases were found to have cytopathic effect (CPE) in the cyst wall and inner contents of the cyst. HPV 60 was detected in these cases by ISH. Two cases of verruca plantaris showing CPE also demonstrated the presence of HPV 60. Of the six HPV type 60 positive cases, four cases also showed CPE in the overlying epidermis.ConclusionAt first, punctate keratotic lesions are induced by HPV 60. HPV 60 induces irregular elongation of the rete ridges into the dermis. Plantar cysts probably form as a result of the traumatic implantation of HPV 60 infected epidermis with irregularly long and narrow rete ridges into the dermis.
Immunopathologic Demonstration of MAC387+ UCHL1+ Cells in Bullous Skin Diseases
Authors: Zou Ming‐xi , Chen Qi‐jie 
Published: April 2015

AbstractImmunopathologic aspects of bullous skin diseases were studied in paraffin sections of thirty‐three skin biopsy specimens utilizing monoclonal antibodies directed against histiocytes, helper/inducer T lymphocytes, and Langerhans cells in skin lesions. Laboratory examinations revealed that either more or fewer of the standard number of helper/inducer T lymphocytes (UCHL1+) were observed in the upper dermis in 84.8% of the cases examined, particularly in the various forms of pemphigus; histiocytes (MAC387+) were also found to occur in the upper dermis in 45.5% of the cases and in blisters in 27.3% of the cases, particularly in pemphigus erythematosus and dermatitis herpetifomis. In 54.5% of all the cases, both UCHL1+ and MAC387+ cell infiltrates predominated in the upper dermis. These findings suggest that a cell‐mediated immune response may also be important in the pathogenesis of bullous skin diseases. It may be possible that antigen from keratinocytes can produce ETAF, and that IL1 activating T lymphocytes and histiocytes also produce IL1 activating T cells, particularly helper/inducer T cells. Such activities would further promote the increase of B cell function and immunoglobulin synthesis.
Clinical and Immunohistochemical Studies of Skin Eruptions: Relationship to Administration of Interferon‐α
Authors: Toyofuku Kazutomo , Imayama Shuhei , Yasumoto Shin‐ichiro , Kiryu Hiromaro , Hori Yoshiaki 
Published: April 2015

AbstractWe observed transient, erythematous skin eruptions in 6 patients during the intravenous administration of interferon (INF)‐α for chronic active hepatitis C. The eruptions appeared 5 to 14 days (mean: 6.8 days) after initiating its administration. They were localized or disseminated and consisted of edematous, erythematous, and/or papular changes. Vesicles and petechiae also appeared in some cases. The eruptions disappeared in 10 to 14 days, despite the continuance of INF‐α and without specific treatment.Histological examination obtained from skin eruptions revealed perivascular infiltration of lymphoid cells, the majority which were CD4‐positive, of the upper dermis. Edematous changes were present in the papillary dermis. Vascular endothelial cells in the upper dermis expressed both intercellular adhesion molecule‐1 (ICAM‐1) and endothelial leukocyte adhesion molecule‐1 (ELAM‐1), while the epidermal keratinocytes produced neither. These findings suggested a nonallergic mechanism for such eruptions.
An Aggressive Spindle Cell Squamous Carcinoma Arising in a Patient with Long‐standing Erythroderma
Authors: Matsumura Tetsuri , Kumakiri Masanobu , Sato‐Matsumura Kazuko C. , Koizumi Hiroko , Ohkawara Akira , Tanifuji Junji , Yoshida Yutaka 
Published: April 2015

AbstractSpindle cell squamous carcinoma (SCSC) of the left hand arising in a patient with longstanding erythroderma is reported. Histopathologically, spindle shaped atypical cells were observed neighboring the cells of well differentiated squamous cell carcinoma. These two types of tumor cells, spindle cells and well differentiated cells, were present side by side and merged into each other. The erythroderma had been present for over 20 years, and both clinical and histopathological findings suggested cutaneous T cell lymphoma, but were not diagnostic for mycosis fungoides, Sézary syndrome, or adult T cell lymphoma. Flow cytometry of peripheral blood cells showed a low CD4/CD8 ratio which suggested impaired T cell function. Multiple metastases of SCSC occurred in a short period and the patient died ten months after his first visit to us. The aggressive course of this case was unusual, and may be due to immunological abnormalities associated with the long standing erythroderma with impaired T cell function.
Functional Morphology of Lesions of Psoriasis Vulgaris Transplanted into Nude Mice at an Early Stage
Authors: Ueda Keiichi , Yanagihara Makoto , Nakagawa Shigemitsu , Fujita Tomozou 
Published: April 2015

AbstractWe examined the growth and differentiation of transplanted psoriatic skin in nude mice at an early stage. Psoriatic lesions from three patients were biopsied. Each biopsied specimen was cut into five blocks, which were individually transplanted to nude mice by the embedding method. Two weeks later, growth and differentiation of transplanted specimens were examined by bromodeoxyuridine (BrdU) labelling method and by electron microscopy. Electron microscopically, basal cells were longitudinally high, and the cellular processes were elongated. Basal laminae were multilayered. In the cytoplasm of the basal cells, many mitochondria, endoplasmic reticula, and ribosomes were seen, and tonofibrillar formation was poor. In cell groups in the upper parts of these lesions, both nuclei and cytoplasm showed degeneration. Psoriatic skin transplanted to nude mice exhibited newly formed psoriatic skin and initial epidermal necrosis. The BrdU labelling method labelled cells scattered in the lower part of the epidermis. Psoriatic skin transplanted to nude mice resembled that before transplantation in both ultrastructural findings and growth pattern.
A Case Report of Infiltrating Ductal Carcinoma Originating from Aberrant Breast Tissue
Authors: Youn Sang No , Kim Young Keun , Park Young Lip 
Published: April 2015

AbstractWe report a case of carcinoma originating from aberrant breast tissue in a 32‐year‐old female who had a soft subcutaneous mass on the anterior axillary fold. Histopathologic examination showed an infiltrating ductal carcinoma of aberrant breast tissue without metastasis to lymph nodes. We believe that subcutaneous nodules of uncertain origin around the periphery of the breast and especially in the axilla should always be viewed with suspicion and treated by early excision.
Calcification of Eccrine Poroma
Authors: Yamamoto Toshiyuki , Irifune Ayumi , Katayama Ichiro , Nishioka Kiyoshi 
Published: April 2015

AbstractTwo cases of eccrine poroma with calcification were described, both of which were those of the Winkelmann‐MacLeod type. They occurred on the face and lower leg. We supposed that calcification in both cases was dystrophic in nature due to minor pressure or injury.The feature of calcification within the nests of eccrine poroma has rarely been reported. However, it seemed to be physiologically consistant that calcification occurs in eccrine poroma which originates from eccrine sweat glands.