bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nutrient competition predicts gut microbiome restructuring under drug perturbations
Authors: Shi Handuo , Newton Daniel P. , Nguyen Taylor H. , Estrela Sylvie , Sanchez Juan , Tu Michael , Ho Po-Yi , Zeng Qinglin , DeFelice Brian , Sonnenburg Justin , Huang Kerwyn Casey 
Published: August 2024

ABSTRACTHuman gut commensal bacteria are routinely exposed to various stresses, including therapeutic drugs, and collateral effects are difficult to predict. To systematically interrogate community-level effects of drug perturbations, we screened stool-derivedin vitrocommunities with 707 clinically relevant small molecules. Across ∼5,000 community–drug interaction conditions, compositional and metabolomic responses were predictably impacted by nutrient competition, with certain species exhibiting improved growth due to adverse impacts on competitors. Changes to community composition were generally reversed by reseeding with the original community, although occasionally species promotion was long-lasting, due to higher-order interactions, even when the competitor was reseeded. Despite strong selection pressures, emergence of resistance within communities was infrequent. Finally, while qualitative species responses to drug perturbations were conserved across community contexts, nutrient competition quantitatively affected their abundances, consistent with predictions of consumer-resource models. Our study reveals that quantitative understanding of the interaction landscape, particularly nutrient competition, can be used to anticipate and potentially mitigate side effects of drug treatment on the gut microbiota.
Altered activity of mPFC pyramidal neurons and parvalbumin-expressing interneurons during social interactions in aMecp2mouse model for Rett syndrome
Authors: Medeiros Destynie , Polepalli Likhitha , Li Wei , Pozzo-Miller Lucas 
Published: August 2024

ABSTRACTSocial memory impairments inMecp2knockout (KO) mice result from altered neuronal activity in the monosynaptic projection from the ventral hippocampus (vHIP) to the medial prefrontal cortex (mPFC). The hippocampal network is hyperactive in this model for Rett syndrome, and such atypically heightened neuronal activity propagates to the mPFC through this monosynaptic projection, resulting in altered mPFC network activity and social memory deficits. However, the underlying mechanism of cellular dysfunction within this projection between vHIP pyramidal neurons (PYR) and mPFC PYRs and parvalbumin interneurons (PV-IN) resulting in social memory impairments inMecp2KO mice has yet to be elucidated. We confirmedsocial memory(but notsociability) deficits inMecp2KO mice using a new 4-chamber social memory arena, designed to minimize the impact of the tethering to optical fibers required for simultaneousin vivofiber photometry of Ca2+-sensor signals during social interactions. mPFC PYRs of wildtype (WT) mice showed increases in Ca2+signal amplitude during explorations of a novel toy mouse and interactions with both familiar and novel mice, while PYRs ofMecp2KO mice showed smaller Ca2+signals during interactions only with live mice. On the other hand, mPFC PV-INs ofMecp2KO mice showed larger Ca2+signals during interactions with a familiar cage-mate compared to those signals in PYRs, a difference absent in the WT mice. These observations suggest atypically heightened inhibition and impaired excitation in the mPFC network ofMecp2KO mice during social interactions, potentially driving their deficit in social memory.
Transcriptomic profiling of healthy individual-derived LCLs revealed inter-individual variability towards-hypoxia-responsive pathways
Authors: Singh Dayanidhi , Mehta Komal , Rani Ritu , Agrawal Satyam Kumar , Prasher Bhavana 
Published: August 2024

AbstractHypoxia, or low oxygen levels, affects various developmental, physiological, and pathological processes. It’s been consistently reported that there is an inter-individual variability at genetic and molecular pathways related to oxygen sensing and response. Understanding the underlying variability towards hypoxia sensing and response in health and disease conditions is challenging. ThePrakritistratification method of Ayurveda offers solutions, which classifies healthy individuals into different groups based on multisystem phenotyping. Our lab has previously used this method and provided evidence for the variability in hypoxia responsiveness physiologically among healthy individuals at population levels.Our current study seeks to understand hypoxia sensing and response pathways at cellular levels. We used eight Lymphoblastoid cell lines (LCLs) developed from healthy individuals of extremePrakrititypes. Hypoxia challenge experiments were performed using 0.2% oxygen for 24 and 48 hrs. of chronic hypoxia and captured global transcriptomics profiles. Differentially expressed genes revealed activation of core hypoxia-induced transcriptomic signatures, such as HIF-1⍺ signaling and their metabolic reprogramming in pooled as well as in all Prakriti groups. However, there werePrakriti-specific differences, such as activation of TGF-β mediated ROS and PI3K/AKT/mTOR driven mTORC1complex in Kapha, downregulation of cholesterol homeostasis and regulation of Phosphoinositide biosynthesis in membrane potential observed in the Pitta group. In contrast, ER stress-induced activation of cell survival response via Unfolded protein response in the Vata group. ThePrakritistratification method will provide a novel method to understand inter-individual differences in hypoxia response pathways.SignificanceHypoxia can significantly impact various aspects of our health and well-being. All nucleated cells sense and respond to hypoxia, depending upon their cellular and metabolic activities. Its wide utility and spatiotemporal regulation make it a crucial target to study. We have used thePrakritistratification method of Ayurveda to explore hypoxia sensing and response at cellular levels. Lymphoblastoid Cell Lines (LCLs) developed from the peripheral blood of stratified healthy individuals have been utilized to study the expression level variability at the baseline and hypoxia-induced conditions. The outcomes of our study will be crucial to understanding inter-individual variability in response to hypoxia overlayed baseline variations. Resulting in differential susceptibility towards hypoxic response in health and contributes to understanding variable outcomes in disease conditions. Molecular targets from our study will further be utilized for interventional drug targets in hypoxia-induced disease conditions.
Evidences of an inhibitory effect and other functions of human P53 inEscherichia coli
Authors: Ben Abid Sihem , Yacoubi Ines , Djemal Lamia , Dardouri Mosbeh , Gargouri Ali 
Published: August 2024

AbstractThe human tumor suppressor P53 has so far been shown to inhibit growth in the yeast model as well as in other eukaryotic contexts. Despite a considerable number of sudies involving p53 in bacteria, the question of effects on cell growth and viability has never been explored. In this work, we report similar negative effect on cell viability of the protein expressed inEscherichia colistrain BL21(DE3). This inhibition still needs to be characterized in lights of the distinction between yeast and other organisms with different P53-caused deaths and pathways. Primary tests leaned towards an active p53 in this bacterial context, both under normal and stresseful conditions. Special effects were noticed either with phage infection or antibiotic treatment, using a GST-p53 fusion form. These results open the way for further investigations involving P53 in prokaryote systems.
Pembrolizumab monotherapy for previously treated metastatic HER2-negative breast cancer with germlineAPOBEC3Bdeletion: results of the phase II AUROR study
Authors: Ho Gwo Fuang , Lee Soo Chin , Bustam Anita Zarina , Alip Adlinda , Abdul Satar Nur Fadhlina , Saad Marniza , Abdul Malik Rozita , Lim Siew Eng , Ow Samuel G. W. , Wong Andrea , Chong Wan-Qin , Ang Yvonne L. E. , Lee Audrey Weng Yan , Hasan Siti Norhidayu , Tuan Zaid Nabilah , Law Kian Boon , Toh Yok Yong , Tan Hooi Chiao , Selvam Bawani , Lim Joanna , Pan Jia-Wern , Teo Soo Hwang 
Published: August 2024

AbstractBackgroundA common germline deletion polymorphism in theAPOBEC3Bgene increases the rate of somatic hypermutation in breast cancer, which in turn is associated with greater neoantigen burden and immune activation. This phase II study evaluated the impact of theAPOBEC3Bdeletion polymorphism on the response to pembrolizumab monotherapy in metastatic HER2-negative breast cancer patients.Patients and methodsEligible patients had a confirmed diagnosis of metastatic HER2-negative breast cancer, 1-3 prior lines of therapy, and documented homozygous or heterozygous germline deletion ofAPOBEC3B. Patients received 200 mg of pembrolizumab intravenously every 3 weeks for up to 2 years. The primary endpoint was objective response rate. Secondary endpoints were disease control rate, progression-free survival, and overall survival.ResultsAll enrolled patients (N = 44) were women, 36% had PD-L1-positive tumours, and 62% had received ≥2 previous lines of therapy for metastatic disease. ORR (95% CI) was 20.5% (9.8 – 35.5) in the total and 30.0% (6.7 – 65.3) in the PD-L1-positive populations. Disease control rate (95% CI) was 52.3% (36.7 – 67.5) and 40% (12.2 – 73.8), respectively. Median PFS was 3.1 months (95% CI, 2.1 – 4.3), and 6-month PFS rate was 29.5% (95% CI, 18.7 – 46.6). Median OS was 15.2 months (95% CI, 11.7 – 26.5), and 12-month OS rate was 60.2% (95% CI, 46.5 – 77.7). Treatment-related adverse events occurred in 30 (68.2%) patients, including 1 (2.3%) with grade 3 AE. There were no deaths due to AEs.ConclusionsPembrolizumab monotherapy demonstrated durable antitumour activity in a subset of previously treated metastatic HER2-breast cancer patients with germline APOBEC3B deletion.Clinical trial registrationClinicalTrials.gov,NCT03989089.
Tuning the electronic structure of monolayer
MoS2
towards metal like via vanadium doping
Authors: Maity Dipak , Sharma Rahul , Sahoo Krishna Rani , Lal Ashique , Arenal Raul , Narayanan Tharangattu N. 
Published: August 2024

Doping of two-dimensional layered semiconducting materials is becoming pivotal in tailoring their electronic properties, enabling the development of advanced electronic and optoelectronic devices, where the selection of dopant is important. Here, we demonstrate the potential of substitutional vanadium (V) doping in monolayer molybdenum disulfide (MoS2) lattice in different extents leading to tunable electronic and optoelectronic properties. We found that low-level V doping (∼1 at.%) induces p-type characteristics in otherwise n-type monolayer MoS2, whereas medium-level doping (∼5 at.%) leads to an ambipolar semiconductor. Degenerately doped MoS2 (∼9 at.%) facilitates a transition from semiconducting towards metallic (metal-like) with reduced electrical resistivity (∼4.5 Ωm of MoS2 to ∼2.2×10−5Ωm), low activation energy for transport (∼11 meV), and electric field independent drain current in field effect transistor–based transfer characteristics. A detailed temperature- and power-dependent photoluminescence study along with density functional theory–based calculations in support unravels the emergence of an excitonic transition at ∼850 nm with its intensity dependent on the amount of vanadium. This study shows the potential of V doping in MoS2 for generating multifunctional two-dimensional materials for next generation electronics, optoelectronics, and interconnects with systematic control over its electronic structure in a wide range.

Published by the American Physical Society
2024
The lived experiences of South African Black accountants within the framework of the chartered accountancy profession sector code
Authors: Musundwa Sedzani , Moses Olayinka 
Published: August 2024

PurposeThis study investigates the progress of the Broad-Based Black Economic Empowerment (B-BBEE) Act 2003, and its associated Chartered Accountancy Profession Sector Code. In doing so, we explore why B-BBEE affirmative action has not yet achieved the Code's representational intentions, including systemic subtleties inhibiting this success.Design/methodology/approachUsing semi-structured interviews, we explore the lived experiences of Black aspirant Chartered Accountants (CAs) undertaking articles in global audit firms. The experiences are thematically analysed, embracing a comprehensive theoretical approach that encompasses professional and social closure, as well as boundary work, to adequately understand why affirmative transformational endeavours persistently face uphill tasks. The utilisation of multifaceted theorisation is deemed essential for a more nuanced portrayal of the intricacies inherent in the CA profession in South Africa.FindingsThe narratives presented by Black aspiring CAs unveil a complex web of exclusionary practices entrenched in institutionalised historical, professional, and social contexts. The multifaceted nature of closures, symbolised by racial, cultural, and linguistic factors, significantly impacts the experiences of Black trainees. The findings furthermore show that deliberate intervention beyond compliance with the Government’s framework is necessary for meaningful transformation.Practical implicationsThe paper brings to the fore the current lived experiences of underrepresented Black CAs in global auditing firms. In doing so, these firms are empowered with incremental knowledge of the prevailing challenges and can thus make tangible improvements towards authentic transformation. Additionally, the results help in tracking the advancements made through affirmative action, acting as a feedback loop for future developments in transformation policy.Originality/valueContributing to the critical accounting literature, our study extends scholarship on the barriers faced by CAs and the limitations in their capacity to challenge these obstacles within global audit firms. We offer practical policy-focused recommendations that, if implemented, can address the complex socio-political realities obstructing the success of affirmative action. By sharing first-hand accounts, our study aims to empower auditing firms and other related stakeholders with actionable insights, enabling them to improve genuine inclusivity and foster equitable representation in the accounting profession.
Utilizing FSA conservation loan programs to support farm conservation activities
Authors: Atkinson Sarah A. , Dodson Charles B. , Wengrin Melinda 
Published: August 2024

PurposeThe Farm Service Agency (FSA) conservation loan program was introduced in the 2008 Farm Bill to provide additional credit to assist producers implementing approved Natural Resources Conservation Service (NRCS) conservation projects. This paper explores why this program has been widely underutilized despite an overall increase in United States Department of Agriculture (USDA) Conservation Program participation.Design/methodology/approachThe FSA administrative loan data are merged with NRCS program participation and payments data for 2010–2021. The share of project costs paid by producers and resulting savings achieved by farmers participating in both programs if their cost-share portion was paid by FSA loans are estimated, as well as the impact on farmer conservation spending under different estimates of increased participation.FindingsA significant share of FSA farmers are likely to take advantage of NRCS programs, with the majority of participants paying under $25,000 in cost-share portions. These loans are less suited to guaranteed conservation loans and more appropriate for the discontinued direct conservation loan program. Few FSA borrowers participating in NRCS cost-share programs pay more than $50,000 in cost-share portions. These loans would receive the majority of benefits from interest reduction schemes under the current guaranteed loan program.Practical implicationsOur results and suggestions provide valuable information when discussing the Guaranteed Conservation Loan Program in the 2023 Farm Bill legislation.Originality/valueNo prior research has attempted to merge FSA guaranteed or direct loan data with conservation program participation and payment data, focused on producer cost-share levels or the FSA Guaranteed Conservation Loan Program in the last decade, making this study a valuable contribution to the literature.
Research on the influence of digital human avatar characteristics on brand fans effect
Authors: Xie Jifei , Wu Haoyu , Li Zhe , Ma Lulu , Liu Kexi 
Published: August 2024

PurposeThis study aims to provide supplements to the research on digital human avatar (DHA) and suggestions for brands to use DHA appropriately to build brand fans effect.Design/methodology/approachOn the basis of integrating Avatar theory and Stimulus-organism-response theory, this study obtains data from 733 Chinese respondents aged 18–25 and uses regression analysis and bootstrap analysis to verify the relationships among the variables: DHA characteristics (form realism, behavioral realism and brand alignment) as the independent variables, brand fans effect as the dependent variable, consumer positive emotion as the mediating variable and product type (experience vs search) as the moderating variable.FindingsThe results show that DHA characteristics positively influence brand fans effect and consumer positive emotion, consumer positive emotion positively influences brand fans effect and consumer positive emotion plays a mediating role. Meanwhile, for experience products, the impact of DHA’s form realism and behavioral realism on consumer positive emotion is higher than that of brand alignment; for search products, the impact of DHA’s brand alignment on consumer positive emotion is higher than that of form realism and behavioral realism.Originality/valueThis study enriches and expands the empirical research perspectives and conclusions in the DHA field, improves its research framework and provides suggestions for brands to appropriately use DHA to build brand fans effect.
Are clusters and industrial districts really driving sustainability innovation?
Authors: Hervas-Oliver Jose-Luis , Márquez García Juan Antonio Antonio , F. García-Chamizo , Rojas-Alvarado Ronald 
Published: August 2024

Purpose
The purpose of this study is to explore and conducts a critical literature review to answer a fundamental question in the industrial district literature: are clusters and industrial (clusters/IDs) driving sustainability innovation? By intersecting different yet related strands of literature, the authors take stock of what the authors know about sustainability innovation in clusters/IDs.

Design/methodology/approach
This paper reviews the literature for conceptualizing sustainability innovation in clusters/districts.

Findings
Insights point out that the sustainability innovation process (development and diffusion) in clusters/IDs and their firms couples into mainstream cluster/IDs framework; clusters/IDs enable sustainability innovation through usual mechanisms, fostering collective change toward sustainability innovation, vis-à-vis other settings and strengthening firm sustainability innovation and performance. Sustainability innovation in clusters/IDs requires coupling different multi-scalar institutional systems effectively, and the cooperation of local organizations and policymakers for co-designing dedicated policies. Collective actions are important and firm heterogeneity needs to be considered in the clusters/IDs framework.

Originality/value
This study is original because it provides state-of-the-art on sustainability innovation in clusters/districts, enabling the topic to advance in this direction.
Big data analytics-artificial intelligence and supply chain ambidexterity impacts on corporate image and green communication
Authors: Chen Chin-Tsu , Chen Shih-Chih , Khan Asif , Lim Ming K. , Tseng Ming-Lang 
Published: August 2024

PurposeThe theoretical background bases on the big data analytics-artificial intelligence (BDA-AI) technologies and supply chain ambidexterity (SCAX) in the firms to assess their sustainability endeavors such as green supply chain management (GSCM) to improve their green communication and corporate image.Design/methodology/approachAround 220 participants in the manufacturing firms are participants' industry expertise, diverse roles, and representation as key stakeholders.FindingsThe results show BDA-AI and SCAX affected on GSCM and found the significant relationships with green communication and corporate image. Green communication was discovered to impact corporate image significantly.Originality/valuePrior studies are neglected to address the relationship among the AI, powered by rapid computational and BDA breakthroughs, redefines cognitive tasks, achieving feats previously deemed impossible-making implicit judgments, simulating emotions, and driving operations. This study selects manufacturing firms as respondents due to their forefront of BDA-AI and supply chain ambidexterity adoption to benefit the operational efficiency and competitiveness. The firms intricate supply chains, diverse stakeholders, and strategic emphasis on corporate image make it an ideal context to examine the nuanced impact of these technologies.
IPOs and knowledge management practices: a systematic literature review and future research agenda
Authors: Nirino Niccoló , Battisti Enrico , Erben Michal , Salvi Antonio , Bresciani Stefano 
Published: August 2024

Purpose
The purpose of this paper is to explore the connection between initial public offerings (IPOs) and knowledge management (KM). Specifically, the manuscript critically examines the literature on IPOs and KM underlying how KM practices influence the IPO processes of companies.

Design/methodology/approach
The authors employ a systematic literature review methodology to identify and thematically investigate 21 articles published in journals by the Chartered Association of Business Schools (ranked 2, 3, 4, 4*).

Findings
This research sheds new light on the relevance of KM practices in the context of IPOs. Specifically, the authors identify four crucial aspects concerning companies that opt for an IPO: (i) reasons for IPO and the role of KM; (ii) IPO process and the role of KM; (iii) underpricing and the role of KM; (iv) post-IPO and the role of KM.

Originality/value
This paper shows the pivotal role of effective KM strategies in fostering a successful IPO. Additionally, it provides practical recommendations for companies seeking to effectively harness their intellectual assets during the IPO process.