bluuebunny/crossref_metadata_2025_split huggingface dataset:
Instandhaltungskonzepte für Parkbauten als Ergebnis der Bedarfsplanung
Authors: Bastert Heinrich , Meyer Lars 
Published: June 2014

AbstractBei der Planung sowohl neuer Betonbauwerke als auch für Instandsetzungen bestehender Objekte sind die Aspekte Wartung und Instandhaltung gemäß einschlägigen Normen und Regelwerken von Anfang an zu berücksichtigen. Dies ist sowohl für den Erhalt von Stand‐ und Verkehrssicherheit der Baukonstruktion als auch im Sinne der uneingeschränkten Gebrauchstauglichkeit des Bauwerks unabdingbar. Der Umfang planmäßig erforderlicher Instandhaltungsmaßnahmen hängt dabei wesentlich von der gewählten Baukonstruktion ab. Insbesondere bei Parkbauten in Betonbauweise beeinflusst die gewählte Ausführungsvariante wesentlich die erforderlichen Inspektions‐ und Wartungsmaßnahmen. Diese führen über den Lebenszyklus, neben den daraus unmittelbar entstehenden Kosten, zu unterschiedlichen Nutzungseinschränkungen und damit möglicherweise zu Einnahmeausfällen. Hieraus ergibt sich, dass bereits bei der Konzeption von Parkbauten die diesbezüglichen Erwartungen und Bedürfnisse des Bauherrn bzw. Betreibers berücksichtigt werden sollten. Zu deren systematischer Erfassung bietet sich ein methodisches Vorgehen im Rahmen einer Bedarfsplanung für Parkbauten an, die sich darüber hinaus zur umfassenden Dokumentation der Planungsziele auf Basis definierter Bauherrenwünsche eignet.Maintenance concepts for parking facilities as results of a design briefStructural design of new concrete constructions as well as repair of existing buildings from the very beginning have to follow the relevant standards and guidelines concerning maintenance and repair. This is also necessary to preserve stability, traffic safety, and usability of the building. The designed and required measurements basically depend on the selected construction method or design option. Especially in case of parking facilities built in reinforced concrete the selected design option requires certain inspection and maintenance activities that also concern the commercial viability. The brief for building design or identification of needs is a recommended tool that is appropriate for parking facilities to systematically capture the needs of the client/user and to document them in a design brief.
Mechanische Modelle für mehraxiales Festigkeits‐ und Ermüdungsversagen von Stahlbeton
Authors: Grünberg Jürgen , Göhlmann Joachim , Marx Steffen 
Published: June 2014

AbstractRäumliche Beanspruchungssituationen haben erhebliche Auswirkungen auf die Betonfestigkeit. Das gilt sowohl für monoton steigende als auch für zyklische Belastung. Ausgehend von zwei geeigneten mehraxialen Festigkeitsmodellen werden mechanische Modelle für das Ermüdungsversagen von Normalbeton sowie von hochfestem und ultrahochfestem Beton entwickelt. Für Normalbeton werden in das Fünfparametermodell nach WILLAM/WARNKE zwei Schädigungsvariablen eingeführt, welche über einen energetischen Ansatz die Erfassung der Schädigung aus zyklischer Beanspruchung erlauben. Mit dem darauf aufbauenden schädigungsabhängigen Verlauf der Verzerrungsentwicklung können in numerischen Simulationen von Tragstrukturen Spannungsumlagerungen realitätsnah abgebildet werden. Für die Beschreibung des Ermüdungsversagens von hochfestem und ultrahochfestem Beton ist das Dreiphasenmodell nach GRÜNBERG wesentlich besser geeignet als das Fünfparametermodell. Verschiedene Bereiche des Modells wurden anhand mehraxialer Laborversuche kalibriert. Zur vollständigen Validierung sind weitere Versuche erforderlich, besonders im Bereich hoher Lastwechselzahlen.Mechanical Models for concrete failure under monotonically increasing as well as cyclic multiaxial loadingTriaxial stress states have a considerable effect on the material strength of concrete in case of both, monotonically increasing as well as cyclic loads. Based on two suitable multiaxial strength models mechanical models for the fatigue failure of normal strength concrete respectively high‐performance and ultra‐high‐performance concrete are developed. The five parameter model by WILLAM/WARNKE is used for normal strength concrete. Two energy based damage variables were introduced into the model, which allow the mathematical description of damage from cyclic loading. Using this model in numerical simulations of structures the evolution of strains and the damage‐depending redistribution of stresses can be displayed realistically. For the description of the fatigue failure of high‐performance and ultra‐high‐performance concrete, the three‐phases model by GRÜNBERG is more appropriate than the five‐parameter model. Several areas of the model were calibrated using triaxial laboratory tests. To fully validate the model, further tests are required, especially in the range of high numbers of load cycles.
Combined multispectroscopic and molecular docking investigation on the interaction between delphinidin‐3‐O‐glucoside and bovine serum albumin
Authors: Zuo Huijun , Tang Lin , Li Shu , Huang Junwei 
Published: June 2014

ABSTRACTAnthocyanin is one of the flavonoid phytopigments with specific health benefits. The interaction between delphinidin‐3‐O‐glucoside (D3G) and bovine serum albumin (BSA) was investigated by fluorescence spectroscopy, synchronous fluorescence spectroscopy, three‐dimensional fluorescence spectroscopy, ultraviolet‐visible absorption spectroscopy, circular dichroism spectroscopy and molecular modeling. D3G effectively quenched the intrinsic fluorescence of BSA via static quenching. The number of binding sites and binding constant Ka were determined, and the hydrogen bonds and van der Waals forces played major roles in stabilizing the D3G–BSA complex. The distance r between donor and acceptor was obtained as 2.81 nm according to Förster's theory. In addition, the effects of pH and metal ions on the binding constants were discussed. The results studied by synchronous fluorescence, three‐dimensional fluorescence and circular dichroism experiments indicated that the secondary structures of the protein has been changed by the addition of D3G and the α‐helix content of BSA decreased (from 56.1% to 52.4%). Furthermore, the study of site marker competitive experiments and molecular modeling indicated that D3G could bind to site I of BSA, which was in the large hydrophobic cavity of subdomain IIA. Copyright © 2014 John Wiley & Sons, Ltd.
Defining an optimal stromal derived factor‐1 presentation for effective recruitment of mesenchymal stem cells in 3D
Authors: Iannone Maria , Ventre Maurizio , Pagano Gemma , Giannoni Paolo , Quarto Rodolfo , Netti Paolo Antonio 
Published: June 2014

ABSTRACTIn “situ” tissue engineering is a promising approach in regenerative medicine, envisaging to potentiate the physiological tissue repair processes by recruiting the host's own cellular progenitors at the lesion site by means of bioactive materials. Despite numerous works focused the attention in characterizing novel chemoattractant molecules, only few studied the optimal way to present signal in the microenvironment, in order to recruit cells more effectively. In this work, we have analyzed the effects of gradients of stromal derived factor‐1 (SDF‐1) on the migratory behavior of human mesenchymal stem cells (MSCs). We have characterized the expression of the chemokine‐associated receptor, CXCR4, using cytofluorimetric and real‐time PCR analyses. Gradients of SDF‐1 were created in 3D collagen gels in a chemotaxis chamber. Migration parameters were evaluated using different chemoattractant concentrations. Our results show that cell motion is strongly affected by the spatio‐temporal features of SDF‐1 gradients. In particular, we demonstrated that the presence of SDF‐1 not only influences cell motility but alters the cell state in terms of SDF‐1 receptor expression and productions, thus modifying the way cells perceive the signal itself. Our observations highlight the importance of a correct stimulation of MSCs by means of SDF‐1 in order to implement on effective cell recruitment. Our results could be useful for the creation of a “cell instructive material” that is capable to communicate with the cells and control and direct tissue regeneration. Biotechnol. Bioeng. 2014;111: 2303–2316. © 2014 Wiley Periodicals, Inc.
CHOPPI: A web tool for the analysis of immunogenicity risk from host cell proteins in CHO‐based protein production
Authors: Bailey‐Kellogg Chris , Gutiérrez Andres H. , Moise Leonard , Terry Frances , Martin William D. , De Groot Anne S. 
Published: June 2014

ABSTRACTDespite high quality standards and continual process improvements in manufacturing, host cell protein (HCP) process impurities remain a substantial risk for biological products. Even at low levels, residual HCPs can induce a detrimental immune response compromising the safety and efficacy of a biologic. Consequently, advanced‐stage clinical trials have been cancelled due to the identification of antibodies against HCPs. To enable earlier and rapid assessment of the risks in Chinese Hamster Ovary (CHO)‐based protein production of residual CHO protein impurities (CHOPs), we have developed a web tool called CHOPPI, for CHO Protein Predicted Immunogenicity. CHOPPI integrates information regarding the possible presence of CHOPs (expression and secretion) with characterizations of their immunogenicity (T cell epitope count and density, and relative conservation with human counterparts). CHOPPI can generate a report for a specified CHO protein (e.g., identified from proteomics or immunoassays) or characterize an entire specified subset of the CHO genome (e.g., filtered based on confidence in transcription and similarity to human proteins). The ability to analyze potential CHOPs at a genomic scale provides a baseline to evaluate relative risk. We show here that CHOPPI can identify clear differences in immunogenicity risk among previously validated CHOPs, as well as identify additional “risky” CHO proteins that may be expressed during production and induce a detrimental immune response upon delivery. We conclude that CHOPPI is a powerful tool that provides a valuable computational complement to existing experimental approaches for CHOP risk assessment and can focus experimental efforts in the most important directions. Biotechnol. Bioeng. 2014;111: 2170–2182. © 2014 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
High current densities enable exoelectrogens to outcompete aerobic heterotrophs for substrate
Authors: Ren Lijiao , Zhang Xiaoyuan , He Weihua , Logan Bruce E. 
Published: June 2014

ABSTRACTIn mixed‐culture microbial fuel cells (MFCs), exoelectrogens and other microorganisms compete for substrate. It has previously been assumed that substrate losses to other terminal electron acceptors over a fed‐batch cycle, such as dissolved oxygen, are constant. However, a constant rate of substrate loss would only explain small increases in coulombic efficiencies (CEs, the fraction of substrate recovered as electrical current) with shorter cycle times, but not the large increases in CE that are usually observed with higher current densities and reduced cycle times. To better understand changes in CEs, COD concentrations were measured over time in fed‐batch, single‐chamber, air‐cathode MFCs at different current densities (external resistances). COD degradation rates were all found to be first‐order with respect to COD concentration, even under open circuit conditions with no current generation (first‐order rate constant of 0.14 ± 0.01 h−1). The rate of COD removal increased when there was current generation, with the highest rate constant (0.33 ± 0.02 h−1) obtained at the lowest external resistance (100 Ω). Therefore, as the substrate concentration was reduced more quickly due to current generation, the rate of loss of substrate to non‐exoelectrogens decreased due to this first‐order substrate‐concentration dependence. As a result, coulombic efficiencies rapidly increased due to decreased, and not constant, removal rates of substrate by non‐exoelectrogens. These results show that higher current densities (lower resistances) redirect a greater percentage of substrate into current generation, enabling large increase in CEs with increased current densities. Biotechnol. Bioeng. 2014;111: 2163–2169. © 2014 Wiley Periodicals, Inc.
Systems metabolic engineering of Escherichia coli for gram scale production of the antitumor drug deoxyviolacein from glycerol
Authors: Rodrigues André Luis , Becker Judith , de Souza Lima André Oliveira , Porto Luismar Marques , Wittmann Christoph 
Published: June 2014

ABSTRACTDeoxyviolacein is a microbial drug with biological activity against tumors, gram‐positive bacteria, and fungal plant pathogens. Here, we describe an Escherichia coli strain for heterologous production of this high‐value drug from glycerol. Plasmid‐based expression of the deoxyviolacein cluster vioABCE was controlled by the araBAD promoter and induction by L‐arabinose. Through elimination of L‐arabinose catabolism in E. coli, the pentose sugar could be fully directed to induction of deoxyviolacein biosynthesis and was no longer metabolized, as verified by 13C isotope experiments. Deletion of the araBAD genes beneficially complemented with previously described (i) engineering of the pentose phosphate pathway, (ii) chorismate biosynthesis, (iii) tryptophan biosynthesis, (iv) improved supply of L‐serine, (v) elimination of tryptophan repression, and (vi) of tryptophan catabolism. Subsequent screening of the created next‐generation producer E. coli dVio‐8 identified glycerol as optimum carbon source and a level of 100 mg L−1 of L‐arabinose as optimum for induction. Transferred to a glycerol‐based fed‐batch process, E. coli dVio‐8 surpassed the gram scale and produced 1.6 g L−1 deoxyviolacein. With straightforward extraction from culture broth and purification by flash chromatography, deoxyviolacein was obtained at >99.5% purity. Biotechnol. Bioeng. 2014;111: 2280–2289. © 2014 Wiley Periodicals, Inc.
Gas chromatography–electron ionization–mass spectrometry quantitation of valproic acid and gabapentin, using dried plasma spots, for therapeutic drug monitoring in in‐home medical care
Authors: Ikeda Kayo , Ikawa Kazuro , Yokoshige Satoko , Yoshikawa Satoshi , Morikawa Norifumi 
Published: June 2014

ABSTRACTA simple and sensitive gas chromatography–electron ionization–mass spectrometry (GC‐EI‐MS) method using dried plasma spot testing cards was developed for determination of valproic acid and gabapentin concentrations in human plasma from patients receiving in‐home medical care. We have proposed that a simple, easy and dry sampling method is suitable for in‐home medical patients for therapeutic drug monitoring. Therefore, in the present study, we used recently developed commercially available easy handling cards: Whatman FTA DMPK‐A and Bond Elut DMS. In‐home medical care patients can collect plasma using these simple kits. The spots of plasma on the cards were extracted into methanol and then evaporated to dryness. The residues were trimethylsilylated using N‐methyl‐N‐trimethylsilyltrifluoroacetamide. For GC‐EI‐MS analysis, the calibration curves on both cards were linear from 10 to 200 µg/mL for valproic acid, and from 0.5 to 10 µg/mL for gabapentin. Intra‐ and interday precisions in plasma were both ≤13.0% (coefficient of variation), and the accuracy was between 87.9 and 112% for both cards within the calibration curves. The limits of quantification were 10 µg/mL for valproic acid and 0.5 µg/mL for gabapentin on both cards. We believe that the present method will be useful for in‐home medical care. Copyright © 2014 John Wiley & Sons, Ltd.
High expression of L‐type amino acid transporter 1 as a prognostic marker in bile duct adenocarcinomas
Authors: Yanagisawa Nobuyuki , Hana Kiyomi , Nakada Norihiro , Ichinoe Masaaki , Koizumi Wasaburo , Endou Hitoshi , Okayasu Isao , Murakumo Yoshiki 
Published: June 2014

AbstractOncocytic L‐type amino acid transporter (LAT) 1 may be a prognostic indicator and target of new molecular therapeutic agents against malignancies. To investigate whether LAT1 expression influence the outcomes of patients with bile duct cancer, the expression of LAT1, LAT2, CD98, and Ki‐67 was investigated immunohistochemically in 134 surgically resected bile duct adenocarcinomas, including 84 distal extrahepatic bile duct adenocarcinomas, 21 hilar cholangiocarcinomas, 15 intrahepatic cholangiocarcinomas, and 14 ampullary adenocarcinomas. LAT1 expression was weakly correlated with CD98 expression and Ki‐67 labeling index (LI). Kaplan–Meier analysis showed a significant difference in prognosis between patients with bile duct adenocarcinomas having LAT1‐high and ‐low scores, whereas LAT2 and CD98 expression and Ki‐67 LI were not predictive of poor prognosis. Prognosis tended to be worse in patients having tumors with LAT1‐high/LAT2‐low than LAT1‐low/LAT2‐high scores (P = 0.0686). Multivariable analyses revealed that LAT1 expression, surgical margin, pT stage were independent prognostic factors. In conclusion, aberrant overexpression of LAT1 in bile duct adenocarcinoma predicts poor prognosis, suggesting that LAT1 may be a potential target of anticancer therapy.
A case of neonatal Jeune syndrome expanding the phenotype
Authors: Drera Bruno , Ferrari Daniela , Cavalli Pietro , Poggiani Carlo 
Published: June 2014

Key Clinical MessageWe report the case of a premature, very low birth weight, newborn with stigmata of Jeune syndrome, a rare skeletal dysplasia, and marked renal involvement (i.e. remarkable prenatal oligohydramnios, histologic nephronophthisis‐like pattern, macroscopic renal cysts, and renal failure), expanding the phenotype consistent with the continuum of syndromic ciliopathies.
Efficient Water Oxidation Catalyzed by Mononuclear Ruthenium(II) Complexes Incorporating Schiff Base Ligands
Authors: Li Ting‐Ting , Chen Yong , Li Fu‐Min , Zhao Wei‐Liang , Wang Chuan‐Jun , Lv Xiao‐Jun , Xu Quan‐Qing , Fu Wen‐Fu 
Published: June 2014

AbstractFour new charge‐neutral ruthenium(II) complexes containing dianionic Schiff base and isoquinoline or 4‐picoline ligands were synthesized and characterized by NMR and ESI‐MS spectroscopies, elemental analysis, and X‐ray diffraction. The complexes exhibited excellent chemical water oxidation activity and high stability under acidic conditions (pH 1.0) using (NH4)2Ce(NO3)6 as a sacrificial electron acceptor. The high catalytic activities of these complexes for water oxidation were sustained for more than 10 h at low concentrations. High turnover numbers of up to 3200 were achieved. A water nucleophilic attack mechanism was proposed. A RuVO intermediate was detected during the catalytic cycle by high‐resolution mass spectrometry.
S=1/2 One‐Dimensional Random‐Exchange Ferromagnetic Zigzag Ladder, Which Exhibits Competing Interactions in a Critical Regime
Authors: Herringer Susan N. , Deumal Mercè , Ribas‐Arino Jordi , Novoa Juan J. , Landee Christopher P. , Wikaira Jan L. , Turnbull Mark M. 
Published: June 2014

AbstractThe synthesis, crystal structure, and magnetic properties (from a combined experimental and First‐Principles Bottom‐Up theoretical study) of the new compound catena‐dichloro(2‐Cl‐3Mpy)copper(II), 1, [2‐Cl‐3Mpy=2‐chloro‐3‐methylpyridine] are described and rationalized. Crystals of 1 present well isolated magnetic 1D chains (no 3D order was experimentally observed down to 1.8 K) and magnetic frustration stemming from competing ferromagnetic nearest‐neighbor (JNN) interactions and antiferromagnetic next‐nearest neighbor (JNNN) interactions, in which α=JNNN/JNN <−0.25. These magnetic interactions give rise to a unique magnetic topology: a two‐leg zigzag ladder composed of edge‐sharing up‐down triangles with antiferromagnetic interactions along the rails and ferromagnetic interactions along the zigzag chain that connects the rails. Crystals of 1 also present a random distribution of the 2‐Cl‐3Mpy groups, which are arranged in two different orientations, each with a 50 % occupancy. This translates into a random static structural disorder within each chain by virtue of which the value of the JNN magnetic interactions can randomly take one of the following three values: 53, 36, and 16 cm−1. The structural disorder does not affect the JNNN value, which in all cases is approximately −9 cm−1. A proper statistical treatment of this disorder provides a computed magnetic susceptibility curve that reproduces the main features of the experimental data.
Preparation and Regioselective Metalation of Bis(trimethylsilyl)methyl‐Substituted Aryl Derivatives
Authors: Werner Veronika , Klatt Thomas , Fujii Masaya , Markiewicz Jenifer , Apeloig Yitzhak , Knochel Paul 
Published: June 2014

AbstractA range of bis(trimethylsilyl)methyl‐substituted aryl derivatives was prepared by using a Kumada–Corriu cross‐coupling reaction. The regioselective metalation of the resulting bis(trimethylsilyl)methyl‐substituted aryl derivatives bearing this bulky silyl group allowed the generation of functionalized aromatics. A regioselective switch in the presence or in the absence of the bis(trimethylsilyl)methyl group has been demonstrated. Furthermore, this silyl group was converted into a formyl group or a styryl group, enhancing the scope of application of such bis(trimethylsilyl)methyl‐substituted arenes.
Boron Diiminate with Aggregation‐Induced Emission and Crystallization‐Induced Emission‐Enhancement Characteristics
Authors: Yoshii Ryousuke , Hirose Amane , Tanaka Kazuo , Chujo Yoshiki 
Published: June 2014

AbstractOrganoboron complexes having aggregation‐ and crystalization‐induced emission properties are presented. The series of boron diiminates were synthesized and the emission behaviors of the synthesized compounds were investigated. Finally, it was found that significant enhancement of the emission depended on the crystallinity in the solid states.
ChemInform Abstract: Different Structural Models of YB2C2 and GdB2C2 on the Basis of Single‐Crystal X‐Ray Data.
Authors: Reckeweg Olaf , DiSalvo Francis J. 
Published: June 2014

AbstractYB2C2 and GdB2C2 are prepared from the elements by arc melting and subsequent annealing at 900 °C (3 d) and characterized by single crystal XRD.
ChemInform Abstract: A Nitrate Nonlinear Optical Crystal Pb16(OH)16(NO3)16 with a Large Second‐Harmonic Generation Response.
Authors: Chang Lixian , Wang Li , Su Xin , Pan Shilie , Hailili Reshalaiti , Yu Hongwei , Yang Zhihua 
Published: June 2014

AbstractColorless block crystals of the title compound are hydrothermally prepared from aqueous mixtures of Pb(NO3)2 and LiOOH (autoclave, 200 °C, 72 h, 92% yield).
ChemInform Abstract: Preparation and Characterization of Transparent, Photoluminescent MgAl2O4:Eu2+ Ceramics.
Authors: Waetzig Katja , Kinski Isabel 
Published: June 2014

AbstractThe title ceramics is prepared by sintering mixtures of commercially available MgAl2O4 powder and a solution of Eu(NO3)3 in EtOH (1100 °C, 5 h) followed by hot isostatic pressing (Ar, 1500 °C, 180 MPa, 15 h).
ChemInform Abstract: Good Thermoelectric Performance in Strongly Correlated System SnCCo3 with Antiperovskite Structure.
Authors: Lin Shuai , Tong Peng , Wang Bosen , Lin Jianchao , Huang Yanan , Sun Yuping 
Published: June 2014

AbstractThe magnetic, electrical, and thermoelectric properties of ACCo3 (A: Sn, Al, Ga, Ge) and SnCM3 (M: Mn, Fe) are investigated.
ChemInform Abstract: Superconducting Double Perovskite Bismuth Oxide Prepared by a Low‐Temperature Hydrothermal Reaction.
Authors: Kumada Nobuhiro , et al. et al. 
Published: June 2014

AbstractThe double perovskite (Na0.25K0.45)(Ba1.00)3 (Bi1.00)4O12 is hydrothermally synthesized from an aqueous mixture of NaBiO3, Ba(OH)2, and KOH (autoclave, 220 °C) and characterized by powder XRD, TEM, magnetic susceptibility, and electrical resistivity measurements.