bluuebunny/crossref_metadata_2025_split huggingface dataset:
Word-representable graphs from a word’s perspective
Authors: Fleischmann Pamela , Haschke Lukas , Löck Tim , Nowotka Dirk 
Published: August 2024

AbstractWord-representable graphs were introduced in 2008 by Kitaev and Pyatkin in the context of semigroup theory. Graphs are called word-representable if there exists a word with the graph’s nodes as letters such that the letters in the word alternate iff there is an edge between them in the graph. Until today numerous works investigated the word-representability of graphs but mostly from the graph perspective. In this work, we change the perspective to the words, i.e., we take classes of words and investigate the represented graphs. Our first subject of interest are the conjugates of words: we determine exactly which graphs are represented if we rotate the word. Afterwards, we look at k-local words introduced by Day et al. (FSTTCS LIPIcs, 2017) in order to gain more insights into this class of words. Here, we investigate especially which graphs are represented by 1-local words. Lastly, we prove that the language of all words representing a graph is regular. We were also able to characterise k-representable graphs, solving an open problem.
Unveiling the importance of the C-terminus in the sugar acid dehydratase of the IlvD/EDD superfamily
Authors: Ren Yaxin , Vettenranta Elias , Penttinen Leena , Blomster Andberg Martina , Koivula Anu , Rouvinen Juha , Hakulinen Nina 
Published: August 2024

Abstract
Microbial non-phosphorylative oxidative pathways present promising potential in the biosynthesis of platform chemicals from the hemicellulosic fraction of lignocellulose. An L-arabinonate dehydratase from Rhizobium leguminosarum bv. trifolii catalyzes the rate-limiting step in the non-phosphorylative oxidative pathways, that is, converts sugar acid to 2-dehydro-3-deoxy sugar acid. We have shown earlier that the enzyme forms a dimer of dimers, in which the C-terminal histidine residue from one monomer participates in the formation of the active site of an adjacent monomer. The histidine appears to be conserved across the sequences of sugar acid dehydratases. To study the role of the C-terminus, five variants (H579A, H579F, H579L, H579Q, and H579W) were produced. All variants showed decreased activity for the tested sugar acid substrates, except the variant H579L on D-fuconate, which showed about 20% increase in activity. The reaction kinetic data showed that the substrate preference was slightly modified in H579L compared to the wild-type enzyme, demonstrating that the alternation of the substrate preference of sugar acid dehydratases is possible. In addition, a crystal structure of H579L was determined at 2.4 Å with a product analog 2-oxobutyrate. This is the first enzyme-ligand complex structure from an IlvD/EDD superfamily enzyme. The binding of 2-oxobutyrate suggests how the substrate would bind into the active site in the orientation, which could lead to the dehydration reaction.

Key points
• Mutation of the last histidine at the C-terminus changed the catalytic activity of L-arabinonate dehydratase from R. leguminosarum bv. trifolii against various C5/C6 sugar acids.
• The variant H579L of L-arabinonate dehydratase showed an alteration of substrate preferences compared with the wild type.
• The first enzyme-ligand complex crystal structure of an IlvD/EDD superfamily enzyme was solved.

Graphical Abstract
Step-by-step optimization of a heterologous pathway for de novo naringenin production in Escherichia coli
Authors: Gomes Daniela , Rodrigues Joana L. , Rodrigues Ligia R. 
Published: August 2024

Abstract
Naringenin is a plant polyphenol, widely explored due to its interesting biological activities, namely anticancer, antioxidant, and anti-inflammatory. Due to its potential applications and attempt to overcome the industrial demand, there has been an increased interest in its heterologous production. The microbial biosynthetic pathway to produce naringenin is composed of tyrosine ammonia-lyase (TAL), 4-coumarate-CoA ligase (4CL), chalcone synthase (CHS), and chalcone isomerase (CHI). Herein, we targeted the efficient de novo production of naringenin in Escherichia coli by performing a step-by-step validation and optimization of the pathway. For that purpose, we first started by expressing two TAL genes from different sources in three different E. coli strains. The highest p-coumaric acid production (2.54 g/L) was obtained in the tyrosine-overproducing M-PAR-121 strain carrying TAL from Flavobacterium johnsoniae (FjTAL). Afterwards, this platform strain was used to express different combinations of 4CL and CHS genes from different sources. The highest naringenin chalcone production (560.2 mg/L) was achieved by expressing FjTAL combined with 4CL from Arabidopsis thaliana (At4CL) and CHS from Cucurbita maxima (CmCHS). Finally, different CHIs were tested and validated, and 765.9 mg/L of naringenin was produced by expressing CHI from Medicago sativa (MsCHI) combined with the other previously chosen genes. To our knowledge, this titer corresponds to the highest de novo production of naringenin reported so far in E. coli.

Key points
• Best enzyme and strain combination were selected for de novo naringenin production.
• After genetic and operational optimizations, 765.9 mg/L of naringenin was produced.
• This de novo production is the highest reported so far in E. coli.
Complications of cochlear implantation: a decade’s experience
Authors: Mostafa Badr Eldin , El Fiky Lobna 
Published: August 2024

Abstract
Purpose
Surgery for cochlear implantation is becoming increasingly common. It is a precise surgery and carries with it a number of very specific complications. Although uncommon, they can profoundly affect the recipients’ quality of life. We report on our patients’ population and compare with different series.

Methods
This is a retrospective analysis of patients who underwent cochlear implant surgery in our center or who were referred for management of complications between November 2012 and November 2022.

Results
There were 2126 patients aged 9 months-68 years [mean 3.2 yrs] with 147 adults > 18 yrs. The male: female ratio was nearly 1. We are reporting on late complications excluding device failures. There were 186 complications [8.7%], 124 minor complications [66% of the complications, 5.8% of the total population; and 62 major complications [ 33% of the complications and 2.9% of the total]. The commonest minor complication was surgical site infection [16%] and the commonest major complication was flap necrosis and device extrusion [26%].

Conclusion
Complications in our series were comparable to other series from different locations. But there seems to be a difference in the frequency of each complication depending on many factors which must be addressed. Standardization of reporting should be also more homogenized.
Facial soft tissue depth of a contemporary adult Greek population
Authors: Coşkun Gülçin , Fasoula Marina , Bontozoglou Nikolaos 
Published: August 2024

AbstractFacial approximation is a technique that involves constructing the facial muscles and applying a suitable facial soft tissue depth (FSTD) dataset. To date, several FSTD studies have been conducted for varying population groups. This study aims to establish a FSTD dataset of an adult Greek population sample for the first time. The facial depths of subjects were measured on 100 head CT scans of 50 male and 50 female subjects aged from 18 to 99. The 3D head and skull models of subjects were segmented in Amira 6.1 by using histogram method. FSTDs were measured at 22 cranial landmarks (5 mid-sagittal, 17 bilateral). The FSTD dataset was generated by considering the age and sex of subjects. The impact of age and sex on the FSTD was limited. Slight inter-population depth variations were reported. Facial asymmetry calculated between the bilateral landmarks was insignificant for both male and female subjects.
Cerebral oxygenation during immediate fetal-to-neonatal transition and fidgety movements between six to 20 weeks of corrected age: An ancillary study to the COSGOD III trial
Authors: Wolfsberger Christina Helene , Schwaberger Bernhard , Urlesberger Berndt , Scheuchenegger Anna , Avian Alexander , Hammerl Marlene , Kiechl-Kohlendorfer Ursula , Griesmaier Elke , Pichler Gerhard 
Published: August 2024

AbstractFidgety movements provide early information about a potential development of cerebral palsy in preterm neonates. The aim was to assess differences in the combined outcome of mortality and fidgety movements defined as normal or pathological in very preterm neonates according to the group allocation in the randomised-controlled multicentre COSGOD III trial. Preterm neonates of two centres participating in the COSGOD III trial, whose fidgety movements were assessed as normal or pathological at six to 20 weeks of corrected age, were analysed. In the COSGOD III trial cerebral oxygen saturation (crSO2) was measured by near-infrared spectroscopy (NIRS) during postnatal transition and guided resuscitation in preterm neonates randomised to the NIRS-group, whereby medical support was according routine, as it was also in the control group. Fidgety movements were classified in normal or abnormal/absent at six to 20 weeks of corrected age. Mortality and fidgety movements of preterm neonates allocated to the NIRS-group were compared to the control-group. Normal outcome was defined as survival with normal fidgety movements. One-hundred-seventy-one preterm neonates were included (NIRS-group n = 82; control-group n = 89) with a median gestational age of 29.4 (27.4–30.4) and 28.7 (26.7–31.0) weeks in the NIRS-group and the control-group, respectively. There were no differences in the combined outcome between the two groups: 90.2% of the neonates in the NIRS-group and 89.9% in the control-group survived with normal outcome (relative risk [95% CI]; 0.96 [0.31–2.62]).Conclusions: In the present cohort of preterm neonates, monitoring of crSO2 and dedicated interventions in addition to routine care during transition period after birth did not show an impact on mortality and fidgety movements defined as normal or pathological at six to 20 weeks corrected age.

What is Known• Fidgety movements display early spontaneous motoric pattern and may provide early information about a potential development of cerebral palsy in preterm neonates.

What is New • This retrospective observational study of the randomised-controlled multicentre COSGOD III trial is the first study investigating the potential influence of cerebral oxygenation guided resuscitation during postnatal transition period on combined outcome of mortality and fidgety movements up to 20 weeks of corrected age in very preterm neonates.• This study adds to the growing interest of assessing cerebral oxygenation, that monitoring of cerebral oxygen saturation and dedicated interventions during postnatal transition period according to the COSGOD III trial has no significant influence on mortality and fidgety movements defined as normal or pathological in very preterm neonates.
Using fuzzy transforms for neural networks-based wireless localization in outdoor environments
Authors: Solmann Kristjan , Loffredo Rocco , Tomasiello Stefania 
Published: August 2024

AbstractAs neural network-based localization algorithms are becoming popular, there is a need to shorten the training time and the localization time for sustainability and efficiency purposes. To address such issues, the fuzzy transform (or F-transform for short) is employed here for the first time in a neural network-based localization algorithm. The F-transform is a dimensionality reduction method, which has found several applications over the last decade, but it has not been well explored in the form of a prepending layer to a neural network. In this respect, some properties (including the computational cost) of the F-transformed neural scheme are formally discussed here. The performance of the neural network-based approach with and without F-transform, and with a state-of-the-art reduction technique, i.e. the principal component analysis, is evaluated first on simulated data and then on publicly available real-world data. Different neural network architectures have been tried jointly with the above-mentioned reduction techniques. The numerical experiments show the excellent performance of the proposed fuzzy transform-based approach, which can ensure considerable savings in training time and query response time, without significant losses in accuracy.
Clinical outcome of non-surgical root canal treatment using different sealers and techniques of obturation in 237 patients: A retrospective study
Authors: Radwanski Mateusz , Pietrzycka Krystyna , Eyüboğlu Tan Fırat , Özcan Mutlu , Lukomska-Szymanska Monika 
Published: August 2024

Abstract
Objectives
The aim of this retrospective study was to compare the clinical results of two root canal sealers and three obturation techniques used for non-surgical root canal treatment.

Materials and methods
A total of two hundred eighty-three root canal treated teeth in two hundred thirty-seven patients with minimum a 6-month follow-up was included for this study. The canals were filled with three different modes: 1) cold lateral condensation (CLC) and AH Plus Sealer; 2) continuous wave condensation technique (CWC) and AH Plus Sealer, and 3) sealer-based obturation technique (SBO) and AH Plus Bioceramic Sealer. The treatment outcome was analysed based on clinical signs and symptoms, and periapical radiograph (periapical index, PAI).

Results
There were no significant differences in treatment outcome between various sealers and filling techniques applied. The sealer extrusion was found most frequently in the CWC group (60.67%), followed by SBO (59.21%) and CLC (21.19%) with statistically significant differences (p < .05). The initial diagnosis, previous treatment and sealer extrusion (p < .05) were prognostic factors that affected treatment outcome.

Conclusions
Based on the findings of this study, neither the sealer type nor the filling technique affected the treatment success while preoperative diagnosis, previous treatment and sealer extrusion had significant effect on the outcome.

Clinical relevance
A bioceramic sealant applied along with the single-cone technique might be considered as an alternative method in root canal obturation.
The mediating role of social relationships between perceived classroom management and adolescents' attitudes toward school: a multilevel analysis
Authors: Obermeier Ramona , Schlesier Juliane , Heinrichs Karin 
Published: August 2024

AbstractSocial relationships in the school context – particularly teacher-student relationships and peer relationships – are related to students’ positive attitudes toward school, and consequently are of great importance regarding various aspects of learning and instruction. This can be concluded from studies that examine the influence of social integration on motivation, learning emotions, and positive attitudes toward school as an institution. Additionally, social relationships are closely related to the teacher’s style of instruction and student perceptions of the learning environment provided. However, there is little research so far, on the extent to which positive social relationships mediate correlations between aspects of teaching quality i.e., classroom management, and students’ positive attitudes toward school. Both classroom management and school-related social relationships are constructs that are assumed to be perceived similarly by students within one school, who experience similar conditions in regard to the intensity and frequency of common lessons. However, there are few research findings regarding shared assessments of classroom management and it’s correlations with teacher-student relationships by students within one school or class, and to date no results regarding the shared assessments of peer relationships. Therefore, the current study aimed to examine the correlations between students’ individual and aggregated assessments of social relationships in school and classroom management, with their positive attitudes toward school. The sample consisted of N = 453 polytechnic school students in Upper Austria, from which a weighted dataset was derived. The findings from multilevel mediation analyses support the assumption of the mediating role of both teacher-student relationships and peer relationships regarding the correlation between classroom management and students’ positive attitudes toward school. However, significant correlations were found only at the individual student level.
Towards neuroadaptive navigation assistance to reduce spatial de-skilling
Authors: Fabrikant Sara Irina 
Published: August 2024

AbstractMaps have been invaluable navigation aids for millennia and thus have been critical for human survival. The increasing popularity of and high dependence on digital, location-aware assistive navigation technology, however, has been shown to divert our attention from the environment and to negatively influence innate spatial abilities. To mitigate this, neuroadaptive mobile geographic information displays (namGIDs) are proposed that respond in real-time to navigators’ cognitive task demands and wayfinder’s situated visuo-spatial attention needs. In doing so, namGIDs may not only help navigators maintain navigation efficiency but more importantly, also continuously scaffold spatial learning. To do this, the proposed navigation assistance must strike the appropriate balance between welcomed mobility efficiency gains while limiting human spatial deskilling. Leveraging neuroadaptive cartography, we can ensure to remain effective navigators, empowered to explore the world with confidence.
Alpha Neurofeedback Training in Elite Soccer Players Trained in Groups
Authors: van Boxtel Geert J. M. , Denissen Ad J. J. M. , de Groot Joep A. , Neleman Marjolein S. , Vellema Jur , Hart de Ruijter Evelijne M. 
Published: August 2024

AbstractNeurofeedback training is applied in the world of sports as a means to improve athletes’ performance. Training sessions are usually organized on an individual basis, one at a time. Here we investigated if the training could also be organized in groups. Forty-one national-level football (soccer) players (26 females, 15 males) carried out training sessions simultaneously in groups of up to 13, using a wearable device with Bluetooth connection, during their regular training hours at the club. It was possible to obtain good EEG measurements using this setup, albeit with a somewhat higher data loss than usual in standard laboratory sessions. The brain’s alpha activity was trained using music-based neurofeedback in a crossover design. A training session consisted of alternating periods of neurofeedback and execution of cognitive tasks. EEG alpha (8–12 Hz) activity was higher in the neurofeedback periods compared to the cognitive task periods, and the reverse was true for beta (13–30 Hz) activity. The training program resulted in an increase of 34% in alpha activity associated with the training, and improved the athletes’ performance on task switching and mental rotation tasks. In addition, self-reported sleep duration, as well as scores on the Being in Shape questionnaire (Feeling of Control and Flow) also improved. This study shows that neurofeedback training is feasible in groups of athletes, which can stimulate its application in team sports.
Statistical Inference of Upstream Turbulence Intensity for the Flow Around a Bluff Body with Massive Separation
Authors: Moussie Tom , Errante Paolo , Meldi Marcello 
Published: August 2024

AbstractThe Benchmarck on the Aerodynamics of a Rectangular 5:1 Cylinder is studied using a data-driven technique which bridges numerical simulation and available experimental results. Because of intrinsic features of the tools used for investigation, in particular in terms of set-up and boundary conditions, significant discrepancies have been observed in the literature when comparing experimental and numerical results. An approach based on the Ensemble Kalman Filter is here used to optimize a synthetic turbulent inlet used as boundary condition in the numerical calculation, in order to reduce the discrepancy with the available experiments. The data-driven method successfully optimizes the boundary condition features, which produce a significant improvement of the accuracy in the prediction of the flow. These findings open perspectives of application towards the analysis of realistic cases, where boundary conditions are complex and usually unknown.
The Space for Religion and Spirituality in Business, Management, and Entrepreneurship Education: Reflections on the Main Contributions and Tentative Directions
Authors: Moya Soledad , Toledano Nuria 
Published: August 2024

AbstractThe business, management, and entrepreneurship (BME) education provided by universities is believed to influence the way in which organisations are managed in the business world. In recent decades, it has been criticised for its focus on profit and short-term goals, while new approaches inspired by the humanities, particularly religious and spiritual traditions, have been proposed. Although research in this area is growing, it is scattered. In this study, we systematically review the existing literature to deepen our understanding of this emergent stream of research. From a sample of 216 papers, 51 articles from 1996 to 2023, selected from the Web of Science and Scopus databases, are analysed. Overall, the findings highlight that the literature on this topic is predominantly theoretical and suggest that some of the wisdom embedded in religion and spirituality might be considered to inform different discussions in BME courses, with a prominent application of sacred and ancient texts in the teaching of business ethics. Future research avenues are discussed in relation to a variety of themes and pedagogical and empirical approaches that can benefit scholars and advance this area of study.
Tumor microenvironment dynamics in oral cancer: unveiling the role of inflammatory cytokines in a syngeneic mouse model
Authors: Tobe-Nishimoto Ayano , Morita Yoshihiro , Nishimura Junya , Kitahira Yukiko , Takayama Shun , Kishimoto Satoko , Matsumiya-Matsumoto Yuka , Matsunaga Kazuhide , Imai Tomoaki , Uzawa Narikazu 
Published: August 2024

AbstractThe process of cervical lymph node metastasis is dependent on the phenotype of the tumor cells and their interaction with the host microenvironment and immune system; conventional research methods that focus exclusively on tumor cells are limited in their ability to elucidate the metastatic mechanism. In cancer tissues, a specialized environment called the tumor microenvironment (TME) is established around tumor cells, and inflammation in the TME has been reported to be closely associated with the development and progression of many types of cancer and with the response to anticancer therapy. In this study, to elucidate the mechanism of metastasis establishment, including the TME, in the cervical lymph node metastasis of oral cancer, we established a mouse-derived oral squamous cell carcinoma cervical lymph node highly metastatic cell line and generated a syngeneic orthotopic transplantation mouse model. In the established highly metastatic cells, epithelial-mesenchymal transition (EMT) induction was enhanced compared to that in parental cells. In the syngeneic mouse model, lymph node metastasis was observed more frequently in tumors of highly metastatic cells than in parental cells, and Cyclooxygenase-2 (COX-2) expression and lymphatic vessels in primary tumor tissues were increased, suggesting that this model is highly useful. Moreover, in the established highly metastatic cells, EMT induction was enhanced compared to that in the parent cell line, and CCL5 and IL-6 secreted during inflammation further enhanced EMT induction in cancer cells. This suggests the possibility of a synergistic effect between EMT induction and inflammation. This model, which allows for the use of two types of cells with different metastatic and tumor growth potentials, is very useful for oral cancer research involving the interaction between cancer cells and the TME in tumor tissues and for further searching for new therapeutic agents.