bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utility of the new rheumatoid arthritis 2010 ACR/EULAR classification criteria in routine clinical care
Authors: Kennish Lauren , Labitigan Monalyn , Budoff Sam , Filopoulos Maria T , McCracken W Andrew , Swearingen Christopher J , Yazici Yusuf 
Published: October 2012

ObjectivesThe new 2010 American College of Rheumatology/European League Against Rheumatism (ACR/EULAR) criteria for rheumatoid arthritis (RA) have been designed to classify early onset RA, but has not been studied to identify RA in patients with arthritis seen in routine clinical care where correct ‘classification’ of patients, when they are not selected for having RA would be important.DesignProspective, consecutive patients cohort.SettingOutpatient clinic of a university rheumatology centre.ParticipantsA total of 126 patients with joint symptoms were consecutively recruited.InterventionsThe ACR/EULAR RA criteria were applied, with questions followed by a targeted musculoskeletal exam. The gold standard for the diagnosis of RA was the primary rheumatologist's diagnosis.Primary outcome measureNumber of patients with non-RA diagnosis who were classified as having RA by the new classification criteria.ResultsThe sensitivity and specificity of the 2010 criteria in classifying RA were 97% and 55%, respectively, compared with the 1987 RA criteria which were 93% and 76%, respectively. The 2010 criteria as applied to this group of patients had a poorer positive predictive (44% vs 61%) and a similar negative predictive value (98% vs 97%) compared with the 1987 criteria. More specifically, 66.7% of systemic lupus erythematosus patients, 50% of osteoarthritis, 37.5% of psoriatic arthritis and 27.2% of others fulfilled the new criteria and could have been classified as RA.ConclusionsIn this, we believe, the first study to examine the new 2010 ACR/EULAR RA criteria among consecutive patients seen in routine care, we found the criteria to have low specificity, and therefore incorrectly label those as having RA when, in fact, they may have a different type of inflammatory arthritis. Physicians need to be aware of this when applying the new criteria for classifying their patients for any purpose.
Does prognosis and socioeconomic status impact on trust in physicians? Interviews with patients with coronary disease in South Australia: Table 1
Authors: Meyer Samantha B , Ward Paul R , Jiwa Moyez 
Published: October 2012

ObjectivesThere is concern across a range of healthcare settings worldwide that trust in physicians is declining. Decreased trust may lead to lesser tolerance of prognosis uncertainty and an increased demand for tests, referrals and second opinions. Literature suggests that there has been a recent cultural shift towards decreased trust in, and increased questioning of, medical advice. We investigated the impact of varying prognosis and socioeconomic status (SES) on trust in physicians, and patient questioning of medical advice.DesignSemistructured, audio-recorded transcribed interviews were conducted. The interview schedule was developed with reference to the Health Belief Model. Interviews were conducted between October 2008 and September 2009.SettingParticipants were recruited via general practitioner clinics and hospital cardiac rehabilitation programmes.ParticipantsParticipants consisted of patients either receiving preventive treatment or active treatment for established cardiovascular disease.Outcome measuresA coding structure was developed based on the aim of the research, to investigate the impact of varying prognosis and SES on trust in physicians.ResultsOlder participants are more likely than their younger counterparts to be unquestioning of medical advice. Higher SES participants are more likely to question medical advice than lower SES participants. Also, unlike primary prevention participants, established pathology increased participants’ trust, or decreased questioning behaviour. Participants who perceived themselves at risk of a poor or uncertain outcome were unlikely to doubt medical advice.ConclusionsBlind trust in physicians remains strong in older participants, participants who perceive their prognosis to be uncertain and a proportion of lower SES participants. This is important for practitioners in terms of patient agency and points to the importance of moral and ethical practice. However, physicians also need to be aware that there are a growing proportion of patients for whom trust needs to be developed, and cannot be assumed.
The Intention to Share: Psychological Investigation of Knowledge Sharing Behaviour in Online Communities
Authors: Alajmi Bibi M. 
Published: October 2012

This research aims to provide an understanding of knowledge sharing behaviour through the adaptation of two major theories, the Theory of Reasoned Action (TRA) and the Theory of Planned Behaviour (TpB) which were developed by Martin Fishbein and Icek Ajzen from social psychology. Exploring knowledge sharing from a social psychological perspective provides an understanding of the process an individual goes through to make the decision to share his/her knowledge with others as well as the different psychological factors facilitating or hindering knowledge sharing behaviour. Research findings are based on a web-survey of 158 group members of one online community of educators, Tapped In (TI). TI is a professional online community developed and supported by the Stanford Research Institute (SRI International), and directed to enhancing the quality of teaching by developing web-based opportunities of learning and engaging.Proposing an extended theoretical model of knowledge sharing behaviour in online community, this research tested the predictive power of five major variables on the individuals' intention to share expertise and knowledge. Variables included attitude, subjective norms, descriptive norms, controllability and knowledge sharing self-efficacy (KSSE). The study found that normative pressures, including subjective norms and descriptive norms, had a strong influence on the formation of the individual's intention to share in this specific online community. Knowledge sharing self-efficacy also was found to significantly account for explaining the individual's motivation to share his/her knowledge with other members. Attitude and controllability were not found to have significant impacts on the formation of intention.
A Numerical Modeling Study of the Propagation of Idealized Sea-Breeze Density Currents
Authors: Robinson F. J. , Patterson M. D. , Sherwood S. C. 
Published: October 2012

AbstractSea breezes are often modeled as a wave response to transient heating in a stratified environment. They occur, however, as density currents with well-defined fronts, the understanding of which rests primarily on experiments and theory that do not include the stratification within and above the current and the steady heat input at the land surface. These gaps are investigated here via a sequence of idealized 2D density current simulations, progressing from the simplest classical case to more realistic surface heating and stratification.In the classical situation where the entire horizontal density contrast is imposed initially, the front quickly attains a constant speed determined by traditional formulas based on the density contrast across the front and the current depth, or by the amount of heat needed to produce it from an initially barotropic fluid. However, these diagnostic and prognostic tools fail completely if the current is driven by a gradual input of heat, analogous to a real sea-breeze situation. In this case the current accelerates slowly at first, remaining much slower than would be expected based on classical formulas.The motion of a classical density current is mostly inertial, with accelerations occurring at the current head; while in the continuously heated case, the entire current accelerates, requiring interior body forces to develop slowly owing to heating of the density current itself. This explains why observed sea-breeze fronts propagate more slowly than predicted from classical formulas, and may help to explain why larger landmasses, where fronts have more time to accelerate, often experience stronger convective storms when triggered by sea-breeze effects.
A Numerical Simulation Study of the Effects of Anvil Shading on Quasi-Linear Convective Systems
Authors: Oberthaler Andrew J. , Markowski Paul M. 
Published: October 2012

Abstract
Numerical simulations are used to investigate how the attenuation of solar radiation by the intervening cumulonimbus cloud, particularly its large anvil, affects the structure, intensity, and evolution of quasi-linear convective systems and the sensitivity of the effects of this “anvil shading” to the ambient wind profile. Shading of the pre-gust-front inflow environment (as opposed to shading of the cold pool) has the most important impact on the convective systems. The magnitude of the low-level cooling, associated baroclinicity, and stabilization of the pre-gust-front environment due to anvil shading generally increases as the duration of the shading increases. Thus, for a given leading anvil length, a slow-moving convective system tends to be affected more by anvil shading than does a fast-moving convective system. Differences in the forward speeds of the convective systems simulated in this study are largely attributable to differences in the mean environmental wind speed over the depth of the troposphere.
Anvil shading reduces the buoyancy realized by the air parcels that ascend through the updrafts. As a result, anvil shading contributes to weaker updrafts relative to control simulations in which clouds are transparent to solar radiation. Anvil shading also affects the convective systems by modifying the low-level (nominally 0–2.5 km AGL) vertical wind shear in the pre-gust-front environment. The shear modifications affect the slope of the updraft region and system-relative rear-to-front flow, and the sign of the modifications is sensitive to the ground-relative vertical wind profile in the far-field environment. The vertical wind shear changes are brought about by baroclinic vorticity generation associated with the horizontal buoyancy gradient that develops in the shaded boundary layer (which makes the pre-gust-front, low-level vertical wind shear less westerly) and by a reduction of the vertical mixing of momentum due to the near-surface (nominally 0–300 m AGL) stabilization that accompanies the shading-induced cooling. The reduced mixing makes the pre-gust-front, low-level vertical shear more (less) westerly if the ambient, near-surface wind and wind shear are westerly (easterly).
Comparison of the therapeutic effects of ultrasound-guided platelet-rich plasma injection and dry needling in rotator cuff disease: a randomized controlled trial
Authors: Rha Dong-wook , Park Gi-Young , Kim Yong-Kyun , Kim Min Tae , Lee Sang Chul 
Published: October 2012

Objective: To compare the effects of platelet-rich plasma injection with those of dry needling on shoulder pain and function in patients with rotator cuff disease. Design: A single-centre, prospective, randomized, double-blinded, controlled study. Setting: University rehabilitation hospital. Participants: Thirty-nine patients with a supraspinatus tendon lesion (tendinosis or a partial tear less than 1.0 cm, but not a complete tear) who met the inclusion criteria recruited between June 2010 and February 2011. Intervention: Two dry needling procedures in the control group and two platelet-rich plasma injections in the experimental group were applied to the affected shoulder at four-week intervals using ultrasound guidance. Measurements: The Shoulder Pain and Disability Index, passive range of motion of the shoulder, a physician global rating scale at the six-month follow-up, adverse effects monitoring and an ultrasound measurement were used as outcome measures. Results: The clinical effect of the platelet-rich plasma injection was superior to the dry needling from six weeks to six months after initial injection ( P < 0.05). At six months the mean Shoulder Pain and Disability Index was 17.7 ± 3.7 in the platelet-rich plasma group versus 29.5 ± 3.8 in the dry needling group ( P < 0.05). No severe adverse effects were observed in either group. Conclusions: Autologous platelet-rich plasma injections lead to a progressive reduction in the pain and disability when compared to dry needling. This benefit is certainly still present at six months after treatment. These findings suggest that treatment with platelet-rich plasma injections is safe and useful for rotator cuff disease.
Short-term effects of interferential current electro-massage in adults with chronic non-specific low back pain: a randomized controlled trial
Authors: Lara-Palomo Inmaculada Carmen , Aguilar-Ferrándiz María Encarnación , Matarán-Peñarrocha Guillermo A , Saavedra-Hernández Manuel , Granero-Molina José , Fernández-Sola Cayetano , Castro-Sánchez Adelaida María 
Published: October 2012

Objectives: To analyse the effectiveness of a combined procedure of massage and electrotherapy with interferential current in individuals with chronic non-specific low back pain of mechanical aetiology. Design: A single blinded randomized controlled trial. Setting: Clinical setting. Participants: Sixty-two individuals with chronic non-specific low back pain were randomly assigned to an experimental or control group. For 10 weeks the experimental group underwent treatment comprising 20 sessions (twice a week) of massage with interferential current in the lumbar and dorsal–lumbar area, and the control group received superficial lower back massage (effleurage, superficial pressure and skin rolling). Main outcome variables: Oswestry Disability Index, pain visual analogue scale, Tampa Scale for Kinesiophobia, Roland Morris Disability Questionnaire, McQuade Test, Side Bridge Test, quality of life scores and the range of trunk anteflexion motion, which were all assessed before the treatment and immediately after the last treatment session. Results: The 2 × 2 mixed model ANOVA with repeated measurements showed statistically significant group * time interactions for the visual analogue scale ( F = 12.839; P = 0.001), Oswestry Disability Index ( F = 5.850; P = 0.019), Roland Morris Disability Questionnaire ( F = 8.237; P = 0.006) and quality of life (physical function ( F = 16.792; P = 0.001), physical role ( F = 14.839; P = 0.001) and body pain ( F = 11.247; P = 0.001)). Conclusions: In individuals with chronic non-specific low back pain, interferential current electro-massage achieved a significantly greater improvement in disability, pain and quality of life in comparison to superficial massage after 20 treatment sessions.
Anterior Cruciate Ligament Reconstruction in Adolescent Patients
Authors: Dai Boyi , Butler Robert J. , Garrett William E. , Queen Robin M. 
Published: October 2012

Background: Anterior cruciate ligament (ACL) reinjury rates are high in adolescent patients. Knee braces are commonly used after ACL reconstruction to prevent reinjury during return to sports. Hypothesis: Adolescent patients following ACL injury would demonstrate a decreased vertical ground-reaction force, knee extension moment, knee flexion angle, and knee flexion velocity on the surgical limb when compared with the nonsurgical limb during a side-cutting task. A functional knee extension–resistant brace would decrease the limb asymmetries. Study Design: Controlled laboratory study. Methods: Twenty-three adolescent patients 6 months after ACL reconstruction were recruited for this study. Three-dimensional kinematic and kinetic data were collected bilaterally (surgical, nonsurgical) during a 35° side-cutting task while the patient was wearing and not wearing a functional knee extension–resistant brace (nonbraced, braced) on the surgical limb. Results: The surgical limb demonstrated a significant decrease in peak impact vertical ground-reaction force (2.55 body weight [BW] vs 2.8 BW; P < .01), peak propulsion vertical ground-reaction force (2.15 BW vs 2.3 BW; P < .01), peak knee extension moment (0.13 BW × body height [BH] vs 0.17 BW × BH; P < .01), knee flexion angle at peak knee flexion velocity (27.8° vs 30.0°; P = .01), peak knee flexion angle (44.1° vs 48.5°; P < .01), and peak knee flexion velocity (571.3 deg/sec vs 640.1 deg/sec; P < .01) when compared with the nonsurgical limb during both nonbraced and braced conditions. Bracing increased the initial knee flexion velocity (42.4 deg/sec vs −40.2 deg/sec; P = .01) and decreased the initial knee flexion angle on the surgical limb (13.1° vs 15.7°; P < .01). Bracing also affected kinematics of the nonsurgical limb. Bracing did not decrease the asymmetry between surgical and nonsurgical limbs. Conclusion: Adolescent patients 6 months after ACL reconstruction demonstrated significant kinematic and kinetic asymmetries between the surgical and nonsurgical limbs. The limb asymmetries persisted when the patients were wearing a functional knee brace. There were changes in the surgical knee kinematics with and without bracing, especially near initial ground contact. Clinical Relevance: The limb asymmetries are of concern with regard to injuring the graft or the contralateral limb when the patients return to sport.
Hyperfunctional coagulation factor IX improves the efficacy of gene therapy in hemophilic mice
Authors: Cantore Alessio , Nair Nisha , Della Valle Patrizia , Di Matteo Mario , Màtrai Janka , Sanvito Francesca , Brombin Chiara , Di Serio Clelia , D'Angelo Armando , Chuah Marinee , Naldini Luigi , VandenDriessche Thierry 
Published: October 2012

Abstract
Gene therapy may provide a cure for hemophilia and overcome the limitations of protein replacement therapy. Increasing the potency of gene transfer vectors may allow improvement of their therapeutic index, as lower doses can be administered to achieve therapeutic benefit, reducing toxicity of in vivo administration. Here we generated codon-usage optimized and hyperfunctional factor IX (FIX) transgenes carrying an R338L amino acid substitution (FIX Padua), previously associated with clotting hyperactivity and thrombophilia. We delivered these transgenes to hemophilia B mice by hepatocyte-targeted integration-competent and -defective lentiviral vectors. The hyperfunctional FIX transgenes increased FIX activity reconstituted in the plasma without detectable adverse effects, allowing correction of the disease phenotype at lower vector doses and resulting in improved hemostasis in vivo. The combined effect of codon optimization with the hyperactivating FIX-R338L mutation resulted in a robust 15-fold gain in potency and therefore provides a promising strategy to improve the efficacy, feasibility, and safety of hemophilia gene therapy.
Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes
Authors: Moktali Venkatesh , Park Jongsun , Fedorova-Abrams Natalie D , Park Bongsoo , Choi Jaeyoung , Lee Yong-Hwan , Kang Seogchan 
Published: October 2012

AbstractBackgroundCytochrome P450 proteins (CYPs) play diverse and pivotal roles in fungal metabolism and adaptation to specific ecological niches. Fungal genomes encode extremely variable “CYPomes” ranging from one to more than 300 CYPs. Despite the rapid growth of sequenced fungal and oomycete genomes and the resulting influx of predicted CYPs, the vast majority of CYPs remain functionally uncharacterized. To facilitate the curation and functional and evolutionary studies of CYPs, we previously developed Fungal Cytochrome P450 Database (FCPD), which included CYPs from 70 fungal and oomycete species. Here we present a new version of FCPD (1.2) with more data and an improved classification scheme.ResultsThe new database contains 22,940 CYPs from 213 species divided into 2,579 clusters and 115 clans. By optimizing the clustering pipeline, we were able to uncover 36 novel clans and to assign 153 orphan CYP families to specific clans. To augment their functional annotation, CYP clusters were mapped to David Nelson’s P450 databases, which archive a total of 12,500 manually curated CYPs. Additionally, over 150 clusters were functionally classified based on sequence similarity to experimentally characterized CYPs. Comparative analysis of fungal and oomycete CYPomes revealed cases of both extreme expansion and contraction. The most dramatic expansions in fungi were observed in clans CYP58 and CYP68 (Pezizomycotina), clans CYP5150 and CYP63 (Agaricomycotina), and family CYP509 (Mucoromycotina). Although much of the extraordinary diversity of the pan-fungal CYPome can be attributed to gene duplication and adaptive divergence, our analysis also suggests a few potential horizontal gene transfer events. Updated families and clans can be accessed through the new version of the FCPD database.ConclusionsFCPD version 1.2 provides a systematic and searchable catalogue of 9,550 fungal CYP sequences (292 families) encoded by 108 fungal species and 147 CYP sequences (9 families) encoded by five oomycete species. In comparison to the first version, it offers a more comprehensive clan classification, is fully compatible with Nelson’s P450 databases, and has expanded functional categorization. These features will facilitate functional annotation and classification of CYPs encoded by newly sequenced fungal and oomycete genomes. Additionally, the classification system will aid in studying the roles of CYPs in the evolution of fungal adaptation to specific ecological niches.
Annotated genetic linkage maps of Pinus pinaster Ait. from a Central Spain population using microsatellite and gene based markers
Authors: de Miguel Marina , de Maria Nuria , Guevara M Ángeles , Diaz Luis , Sáez-Laguna Enrique , Sánchez-Gómez David , Chancerel Emilie , Aranda Ismael , Collada Carmen , Plomion Christophe , Cabezas José-Antonio , Cervera María-Teresa 
Published: October 2012

Abstract

Background

Pinus pinaster Ait. is a major resin producing species in Spain. Genetic linkage mapping can facilitate marker-assisted selection (MAS) through the identification of Quantitative Trait Loci and selection of allelic variants of interest in breeding populations. In this study, we report annotated genetic linkage maps for two individuals (C14 and C15) belonging to a breeding program aiming to increase resin production. We use different types of DNA markers, including last-generation molecular markers.

Results
We obtained 13 and 14 linkage groups for C14 and C15 maps, respectively. A total of 211 and 215 markers were positioned on each map and estimated genome length was between 1,870 and 2,166 cM respectively, which represents near 65% of genome coverage. Comparative mapping with previously developed genetic linkage maps for P. pinaster based on about 60 common markers enabled aligning linkage groups to this reference map. The comparison of our annotated linkage maps and linkage maps reporting QTL information revealed 11 annotated SNPs in candidate genes that co-localized with previously reported QTLs for wood properties and water use efficiency.

Conclusions
This study provides genetic linkage maps from a Spanish population that shows high levels of genetic divergence with French populations from which segregating progenies have been previously mapped. These genetic maps will be of interest to construct a reliable consensus linkage map for the species. The importance of developing functional genetic linkage maps is highlighted, especially when working with breeding populations for its future application in MAS for traits of interest.