bluuebunny/crossref_metadata_2025_split huggingface dataset:
Not just what they want, but why they want it
Authors: Price Rebecca Anne , Wrigley Cara , Straker Karla 
Published: April 2015

Purpose
– This paper aims to explore advantages and disadvantages of both traditional market research (TMR) and deep customer insight (DCI) methods to lay the platform for revealing how a relationship between these two domains could be optimised during firm-based innovation.

Design/methodology/approach
– The paper reports on an empirical research study conducted with 13 Australian-based firms engaged in a design-led approach to innovation. Firms were facilitated through a design-led approach where the process of gathering DCIs was isolated and investigated further in comparison to TMR methods.

Findings
– Results show that DCI methods are able to provide fresh, non-obvious ways of understanding customer needs, problems and behaviours that can become the foundation of new business opportunities. Findings concluded that DCI methods provide the critical layer to understand why customers do or do not engage with businesses. Revealing why was not accessible in TMR methods.

Research limitations/implications
– The theoretical outcome of this study is a complementary methods matrix, providing guidance on appropriate implementation of research methods in accordance with a project’s timeline to optimise the complementation of TMR methods with design-led customer engagement methods.

Practical implications
– DCI methods provide fresh, non-obvious ways of understanding customer needs, problems and behaviours that can become the foundation of new business opportunities. It is hoped that those in a position of data collection are encouraged to experiment and use DCI methods to connect with their customers on a meaningful level and translate these insights into value.

Originality/value
– This paper provides original value to a new understanding of how design techniques can be applied to complement and strengthen existing market research strategies. This is crucial in an era where business competition hinges on a subtle and often intimate understanding of customer needs and behaviours.
Variation of xylem vessel diameters across a climate gradient: insight from a reciprocal transplant experiment with a widespread boreal tree
Authors: Schreiber Stefan G. , Hacke Uwe G. , Hamann Andreas 
Published: April 2015

Summary

Xylem vessel diameters represent an important plant hydraulic trait to ensure sufficient water supply from the roots to the leaves. The ability to adjust the hydraulic pathway to environmental cues is key in order to satisfy transpirational demands and maximize growth and survival.

We evaluated the variability of vessel diameters in trembling aspen in a reciprocal transplant experiment. We tested six provenances from three ecological regions of North America planted at four test sites in western Canada. All test sites were established at the same time with the same provenances arranged in a randomized complete block design.

Vessel diameter showed a strong interaction of population and test site suggesting a high degree of phenotypic plasticity in this trait. Gaussian kernel density estimates support plastic as well as genetic contributions in vessel diameter control trending from bimodal distributions at the most northern test site towards unimodal distributions at the warmest and mildest test site.

Furthermore, we used test site‐specific climate data in form of a 2‐year, 5‐year and 10‐year average of 21 directly and derived climatic variables and found that average site‐specific vessel diameters correlated strongly with summer moisture availability. A previously found negative relationship with vessel diameter and tree height in central Alberta was also found at two other boreal test sites but reversed at a wetter and milder sub‐boreal test site.

In summary, vessel diameters were highly plastic in response to different environments and varied with summer moisture availability. The variability of vessel diameter and tree height correlations suggests that vessel diameter alone cannot serve as a reliable proxy for long‐term growth performance beyond boreal environments. Instead, selecting aspen populations with a high degree of plasticity in this trait appears to be the safest option for assisted migration and seed transfer programmes under climate change.
Midregional Proadrenomedullin Predicts Mortality and Major Adverse Cardiac Events in Patients Presenting With Chest Pain: Results From the CHOPIN Trial
Authors: Shah Kevin S. , Marston Nicholas A. , Mueller Christian , Neath Sean‐Xavier , Christenson Robert H. , McCord James , Nowak Richard M. , Vilke Gary M. , Daniels Lori B. , Hollander Judd E. , Apple Fred S. , Cannon Chad M. , Nagurney John , Schreiber Donald , deFilippi Christopher , Hogan Christopher J. , Diercks Deborah B. , Limkakeng Alexander , Anand Inder S. , Wu Alan H. B. , Clopton Paul , Jaffe Allan S. , Peacock W. Frank , Maisel Alan S. 
Published: April 2015

AbstractObjectivesChest pain is a common complaint to emergency departments (EDs) and clinical risk factors are used to predict which patients are at risk for worse outcomes and mortality. The goal was to assess the novel biomarker midregional proadrenomedullin (MR‐proADM) in prediction of mortality and major adverse cardiac events (MACE).MethodsThis was a subanalysis of the CHOPIN study, a 16‐center prospective trial that enrolled 2,071 patients presenting with chest pain within 6 hours of onset. The primary endpoint was 6‐month all‐cause mortality and the secondary endpoint was 30‐day and 6‐month MACE: ED visits or hospitalization for acute myocardial infarction, unstable angina, reinfarction, revascularization, and heart failure.ResultsMR‐proADM performed similarly to troponin (cTnI; c‐statistic = 0.845 and 0.794, respectively) for mortality prediction in all subjects and had similar results in those with noncardiac diagnoses. MR‐proADM concentrations were stratified by decile, and the cohort in the top decile had a 9.8% 6‐month mortality risk versus 0.9% risk for those in the bottom nine deciles (p < 0.0001). MR‐proADM, history of coronary artery disease (CAD), and hypertension were predictors of short‐term MACE, while history of CAD, hypertension, cTnI, and MR‐proADM were predictors of long‐term MACE.ConclusionsIn patients with chest pain, MR‐proADM predicts mortality and MACE in all‐comers with chest pain and has similar prediction in those with a noncardiac diagnosis. This exploratory analysis is primarily hypotheses‐generating and future prospective studies to identify its utility in risk stratification should be considered.
Role of immunotherapy in lung cancer: Preliminary results of new vaccines and immune checkpoint inhibitors
Authors: John Thomas 
Published: April 2015

AbstractThe two dominant approaches to manipulating cancer immunotherapeutics are active, where the immune system is directly stimulated, and passive, where antitumor antibodies stimulate an indirect immune response. At this point, the active approach is receiving more attention in the arena of lung cancer, with ongoing vaccine clinical trials and studies investigating the role of immune checkpoint inhibitors, in particular those that block the programmed death 1(PD‐1) receptor and its ligands. Early results from trials of PD‐1/PD‐L1 ligand inhibitors in nonsmall cell lung cancer are promising, with patients experiencing rapid and durable responses in the first‐, second‐ and third‐line setting as well as in combination with chemotherapy and other immune checkpoint inhibitors. Although the number of patients in these trials is small and the results are preliminary, lung cancer physicians are encouraged that they may soon have agents that confer benefits in excess of those seen with chemotherapy to offer their patients. Further results of ongoing trials are highly anticipated.
Anderson–Hynes pyeloplasty: are we all really on the same page?
Authors: Adam Ahmed , Smith Grahame H. H. 
Published: April 2015

AbstractBackgroundThe Anderson–Hynes pyeloplasty (AHP) is a surgical technique used in the management of pelvi‐ureteric junction obstruction. This operation has been performed for over six decades and has etched its name in the annals of urology. But are we all really referring to the same procedure?MethodsA systematic review of the Ovid MEDLINE(R) and Ovid OLDMEDLINE(R) database from 1946 to 20 June 2014 was performed for the terms ‘Anderson and Hynes’ and ‘Anderson–Hynes’, with later restriction to the title of journal article. Each respective author's descriptive images (figures/photographic) of technique performed was compared with the original procedure. Non‐English figure legends were translated using online translational tools.ResultsIn total, 242 articles were retrieved. Streamlining this search to articles with the above search terms restricted to article title revealed 58 (34 English/24 non‐English) articles. Only 29/58 papers had referenced the original procedure. Operative images were present in 17/58 of the articles claiming to have performed the AHP. Within these papers, only 7/17 articles depicted both the L‐shaped cut and pelvic flap. Diverse variations were observed in the remainder 10/17 articles. This was a worldwide phenomenon, observed more commonly in recent decades and when minimally invasive techniques were used.ConclusionThe AHP is often not performed as was originally described. Over the decades, surgeons have unknowingly attributed variations to this procedure. Based on this review, a novel ‘geometric classification’ system has been proposed to better define the dismembered pyeloplasty.
Laparoscopic nephron sparing surgery: an Australian experience
Authors: Roberts Jay , Wong Jason , Haxhimolla Hodo , Kua Boon 
Published: April 2015

AbstractBackgroundThe objective of this study was to evaluate clinical outcomes from our initial experience with laparoscopic nephron sparing surgery (LNSS) for small renal masses in Australian practice.MethodsA retrospective review was performed on an initial 50 patients undergoing LNSS. All procedures performed between April 2006 and September 2012 were included with median follow‐up of 30 months. Outcomes measured were: positive surgical margin, warm ischaemic time, total operative time, blood transfusion and complications in the first 30 days after surgery.ResultsThe mean age of patients was 57 years. The mean pre‐operative creatinine was 85 μmol/L and the mean post‐operative creatinine was 89 μmol/L. Sixty‐four per cent of the tumours were malignant tumours. The mean size of tumours was 2.5 cm. There were two malignant positive surgical margins on histology. The mean total operative time was 224 min and the mean warm ischaemic time was 24 min. Nine patients had complications with Clavien–Dindo grade III or lower. There was no grade IV or V complication. No patients were lost to follow‐up and there have been no tumour recurrences to date.ConclusionsLNSS is emerging as a viable alternative to open NSS for small renal tumours with lower morbidity and equivalent oncological and functional outcomes. There is, however, a steep learning curve associated with the procedure.
Retracted: MicroRNA‐218 increases cellular sensitivity to Rapamycin via targeting Rictor in cervical cancer
Authors: Li Jing , Li Xiaocui , Wang Jingpu , Wang Yuan , Qiu Haifeng 
Published: April 2015

We previously reported that microRNA‐218 was frequently lost in cervical cancer and restoration of microRNA‐218 increased cellular radio‐sensitivity via inhibiting. Herein, we aim to investigate the effects of microRNA‐218 on cellular response to mTOR inhibition. The expression of microRNA‐218 and Rictor were measured by Taqman PCR and real time PCR in a panel of 15 cervical cancer tissues. MicroRNA‐218 was stably overexpressed in four cervical cancer cell lines and a series of in vitro and in vivo experiments were performed to investigate cellular sensitivity to Rapamycin. In primary cultured cervical cancer cells, the expression of microRNA‐218 was negatively correlated with the mRNA level of Rictor, which predicted cellular sensitivity to Rapamycin (p = 0.002, R2 = 0.6810). In vitro, overexpression of microRNA‐218 significantly reduced the level of Rictor and enhanced the growth‐inhibition, cell cycle arrest, and apoptosis induced by Rapamycin. In vivo, overexpression of microRNA‐218 further enhanced the suppressive effects of Rapamycin on tumor growth. In conclusion, we demonstrated that microRNA‐218 could re‐sensitize cervical cancer to Rapamycin through targeting Rictor. Moreover, patients with loss of microRNA‐218 presented notable resistance to Rapamycin, indicating that microRNA‐218 might be a valid marker for patients‐stratification in future clinical trials.
Laparoscopic repair of Morgagni hernia with composite mesh in an elderly woman: Report of a case
Authors: Ikarashi Masahito , Matsuda Minoru , Murayama Isao , Fujii Masashi , Takayama Tadatoshi 
Published: April 2015

AbstractA 78‐year‐old woman was admitted to another hospital with vomiting. Chest X‐ray showed an abnormal shadow in the lower right lung field, and CT indicated a Morgagni hernia containing the stomach and transverse colon. The patient was transferred to our hospital and underwent laparoscopic surgery. After the hernia contents were repositioned into the abdominal cavity, we repaired the hernia orifice with a prosthetic mesh to achieve a tension‐free repair. There were no complications after the surgery, and there has been no recurrence. The patient has remained free of clinical symptoms since 10 months after the surgery. Laparoscopic repair with a prosthetic mesh for Morgagni hernia is a simple and safety procedure for elderly patients.
Homemade specimen retrieval bag for laparoscopic cholecystectomy: A solution in the time of fiscal crisis
Authors: Stavrou George , Fotiadis Kyriakos , Panagiotou Dimitrios , Faitatzidou Afroditi , Kotzampassi Katerina 
Published: April 2015

AbstractIntroductionDue to the current economic crisis in Greece, major cutbacks on healthcare costs have been imposed, resulting in a shortage of surgical supplies, including laparoscopic materials. In an attempt to reduce costs, we developed a homemade specimen retrieval bag for laparoscopic cholecystectomy.Materials and Surgical TechniqueWe used the polyethylene bag containing the catheter of a Redon drainage set. The bag was cut in half and pleated longitudinally; then, the gallbladder was placed in the bag and removed through the umbilicus with a grasping forceps.DiscussionFrom September 2011 to June 2012, we used our homemade bag on 85 patients undergoing laparoscopic cholecystectomy. No rupture, accidental opening, or bile leak was observed. The learning curve was found to be five cases. Our homemade specimen retrieval bag seems to be a safe, effective, and easy tool for tissue extraction. Further studies need to be conducted to evaluate its full potential.
Validation of the revised international prognostic scoring system (IPSS‐R) in patients with lower‐risk myelodysplastic syndromes: a report from the prospective European LeukaemiaNet MDS (EUMDS) registry
Authors: de Swart Louise , Smith Alex , Johnston Thomas W. , Haase Detlef , Droste Jackie , Fenaux Pierre , Symeonidis Argiris , Sanz Guillermo , Hellström‐Lindberg Eva , Cermák Jaroslav , Germing Ulrich , Stauder Reinhard , Georgescu Otilia , MacKenzie Marius , Malcovati Luca , Holm Mette S. , Almeida Antonio M. , Mądry Krzysztof , Slama Borhane , Guerci‐Bresler Agnes , Sanhes Laurence , Beyne‐Rauzy Odile , Luño Elisa , Bowen David , de Witte Theo 
Published: April 2015

SummaryBaseline characteristics, disease‐management and outcome of 1000 lower‐risk myelodysplastic syndrome (MDS) patients within the European LeukaemiaNet MDS (EUMDS) Registry are described in conjunction with the validation of the revised International Prognostic Scoring System (IPSS‐R). The EUMDS registry confirmed established prognostic factors, such as age, gender and World Health Organization 2001 classification. Low quality of life (EQ‐5D visual analogue scale score) was significantly associated with reduced survival. A high co‐morbidity index predicted poor outcome in univariate analyses. The IPSS‐R identified a large group of 247 patients with Low (43%) and Very low (23%) risk score within the IPSS intermediate‐1 patients. The IPSS‐R also identified 32 High or Very high risk patients within the IPSS intermediate‐1 patients. IPSS‐R was superior to the IPSS for predicting both disease progression and survival. Seventy percent of patients received MDS‐specific treatment or supportive care, including red blood cell transfusions (51%), haematopoietic growth factors (58%) and iron chelation therapy (8%), within 2 years of diagnosis; while 30% of the patients only required active monitoring. The IPSS‐R proved its utility as a more refined risk stratification tool for the identification of patients with a very good or poor prognosis and in this lower‐risk MDS population.
Responder and health‐related quality of life analyses in men with lower urinary tract symptoms treated with a fixed‐dose combination of solifenacin and tamsulosin oral‐controlled absorption system: results from the NEPTUNE study
Authors: Drake Marcus J. , Sokol Roman , Coyne Karin , Hakimi Zalmai , Nazir Jameel , Dorey Julie , Klaver Monique , Traudtner Klaudia , Odeyemi Isaac A. , Oelke Matthias , van Kerrebroeck Philip ,   the NEPTUNE study group
Published: April 2015

ObjectiveTo evaluate the effect of a fixed‐dose combination (FDC) of solifenacin and an oral‐controlled absorption system (OCAS™) formulation of tamsulosin (TOCAS) on health‐related quality of life (HRQoL) in men with lower urinary tract symptoms associated with benign prostatic hyperplasia (LUTS/BPH).Patients and methodsMen with moderate‐to‐severe storage symptoms and voiding symptoms were treated for 12 weeks with a FDC of solifenacin 6 or 9 mg plus TOCAS (0.4 mg), TOCAS monotherapy (0.4 mg) or placebo in a randomised, double‐blind study (NEPTUNE). The co‐primary endpoints were Total Urgency Frequency Score (TUFS) and total International Prostate Symptom Score (IPSS). HRQoL was assessed by several secondary endpoints: IPSS QoL index, overactive bladder questionnaire (OAB‐q), and Patient Global Impression (PGI) scale. The correlation between symptom improvement (TUFS) and HRQoL was assessed by Spearman rank correlation coefficients. Single and double responder analyses, using subjective and objective measures, were also performed.ResultsIn the responder analyses, men treated with a FDC of solifenacin 6 mg plus TOCAS consistently had significantly improved outcomes compared with placebo (8/8 responder analyses performed) and TOCAS (6/8 responder analyses performed). There was a significant correlation (P < 0.001) between the reduction in TUFS and the improvement in HRQoL defined by IPSS QoL score, OAB‐q symptom bother score, PGI overall bladder symptoms and PGI general health.ConclusionsIn men with LUTS/BPH who have moderate‐to‐severe storage symptoms and voiding symptoms, the reduction in symptoms with a once‐daily FDC of solifenacin and TOCAS was associated with consistent patient‐relevant improvements in HRQoL compared with placebo and TOCAS monotherapy.
Immunogenetic control of the intestinal microbiota
Authors: Marietta Eric , Rishi Abdul , Taneja Veena 
Published: April 2015

SummaryAll vertebrates contain a diverse collection of commensal, symbiotic and pathogenic microorganisms, such as bacteria, viruses and fungi, on their various body surfaces, and the ecological community of these microorganisms is referred to as the microbiota. Mucosal sites, such as the intestine, harbour the majority of microorganisms, and the human intestine contains the largest community of commensal and symbiotic bacteria. This intestinal community of bacteria is diverse, and there is a significant variability among individuals with respect to the composition of the intestinal microbiome. Both genetic and environmental factors can influence the diversity and composition of the intestinal bacteria with the predominant environmental factor being diet. So far, studies have shown that diet‐dependent differences in the composition of intestinal bacteria can be classified into three groups, called enterotypes. Other environmental factors that can influence the composition include antibiotics, probiotics, smoking and drugs. Studies of monozygotic and dizygotic twins have proven that genetics plays a role. Recently, MHC II genes have been associated with specific microbial compositions in human infants and transgenic mice that express different HLA alleles. There is a growing list of genes/molecules that are involved with the sensing and monitoring of the intestinal lumen by the intestinal immune system that, when genetically altered, will significantly alter the composition of the intestinal microflora. The focus of this review will be on the genetic factors that influence the composition of the intestinal microflora.
Nucleopolyhedrovirus infection and/or parasitism by Microplitis pallidipes affected haemolymph titre of 20‐hydroxyecdysone in Spodoptera exigua larvae
Authors: Ji X.‐Y. , Wan N.‐F. , Liu J. , Jiang J.‐X. 
Published: April 2015

AbstractThis study examined the physiological effects of joint and separate parasitism and infection by the endoparasitoid Microplitis pallidipes Szépligeti and the nucleopolyhedrovirus (NPV), respectively, on haemolymph 20‐hydroxyecdysone (20‐E) titre in Spodoptera exigua (Hübner) larvae. The results indicated that in parasitized larvae, virus‐infected larvae (5.7 × 103 and 5.7 × 105 OB/ml) and parasitized larvae infected with virus at 5.7 × 105 OB/ml, compared to healthy larvae, the 20‐E all declined during the first 3 days but began to increase from day 4 after treatment, while in jointly parasitized and infected larvae (5.7 × 103 OB/ml), the 20‐E declined during the first 4 days but began to increase on day 5 after treatment. Meanwhile, compared to parasitized larvae, the 20‐E declined during the first 4 days but significantly increased on day 5 in jointly parasitized and infected larvae (5.7 × 103 OB/ml), while significantly increased during the first 2 days but began to decrease from day 3 after treatment in jointly parasitized and infected larvae (5.7 × 105 OB/ml). Finally, in larvae that were both parasitized and virus infected (5.7 × 103 OB/ml), compared to just virus‐infected larvae (5.7 × 103 OB/ml), the 20‐E was lower on days 3 and 4 but higher on other days after treatment; in larvae that were both parasitized and virus infected (5.7 × 105 OB/ml), compared to just virus‐infected larvae (5.7 × 105 OB/ml), the 20‐E was significantly higher at the first 2 days but lower from day 3 after treatment. Our results revealed that 2nd instar larval M. pallidipes in host bodies may release 20‐E into the haemolymph of S. exigua larvae and that NPV infection may stimulate S. exigua to release more 20‐E during its third to fourth instar larval moulting. We found that this stimulatory effect was greater with higher virus concentrations.