bluuebunny/crossref_metadata_2025_split huggingface dataset:
EFFECTS OF SOME S‐ALKYLTHIOURONIUMS AND RELATED COMPOUNDS ON THE OSMOTIC FRAGILITY AND THE MEMBRANE EXPANSION OF HUMAN ERYTHROCYTES
Authors: BERESFORD ROSEMARY A. , FASTIER F.N. 
Published: May 2015

Changes in the osmotic fragility and critical haemolytic volume of human erythrocytes produced by S‐n‐decylthiouronium (S‐10) and related compounds have been studied.
S‐10 had a biphasic action on osmotic fragility, protecting erythrocytes against lysis in low concentrations but producing lysis in a concentration of 1 mm or higher.
Some lower homologues of S‐10 also protected erythrocytes against osmotic lysis, the degree of protection depending on the length of the alkyl chain.
Critical haemolytic volume was increased by anthihaemolytic concentrations of procaine and chlorpromazine but not by antihaemolytic concentrations of S‐10 and related amidines.
It is concluded that S‐10 and its near homologues penetrate and stabilize erythrocyte membranes, potency increasing with the number of methylene groups in the side‐chain up to about ten. The stabilization produced by S‐10 apparently differs from that produced by many other lipid‐soluble depressant drugs. It may be related to a drug‐induced change in the ionic permeability of the membrane.
BINDING OF [3H]‐MUSCIMOL, A POTENT γ‐AMINOBUTYRIC ACID RECEPTOR AGONIST, TO MEMBRANES OF THE BOVINE RETINA
Authors: OSBORNE NEVILLE N. 
Published: May 2015

The binding of [3H]‐muscimol, a potent γ‐aminobutyric acid (GABA) receptor agonist, to crude membrane preparations of bovine retina was studied, using a filtration method to isolate membranebound ligand.
Specific binding was found to be saturable and occurred at two binding sites with affinity constants of 4.3 nm and 38.2 nm.
Binding was sodium‐independent, enhanced by both freezing and Triton X‐100 treatment but abolished with sodium laurylsulphate.
The binding sites demonstrated a high degree of pharmacological specificity, GABA being a potent displacer of [3H]‐muscimol.
A higher degree of [3H]‐muscimol binding was associated with subcellular fractions enriched with photoreceptor synaptosomes rather than with fractions enriched with conventional synaptosomes.
Diabetes quality of care at a university community in Beirut
Authors: Lakkis Najla A. , Mahmassani Dina M. , Hamadeh Ghassan N. 
Published: May 2015

AbstractRationale, aims and objectivesTo assess the quality of diabetes mellitus (DM) care provided by a group of family doctors in Beirut.MethodsAn observational study, conducted at the American University of Beirut Medical Center–Family Medicine Clinics (AUBMC–FMC), examined the electronic health records (EHRs) of the beneficiaries of the Health Insurance Plan at the American University of Beirut (AUB–HIP) who were older than 20 years (n = 9469) in 2009. The eligible population included patients with DM (n = 701). Several provider/patient‐directed interventions were introduced in the late 2000s, including a comprehensive EHRs system with point‐of‐care computer reminders, a provider financial incentive based on an annual performance appraisal ranked against that of the US Healthcare Effectiveness Data and Information Set (HEDIS) Comprehensive Diabetes Care indicators, as well as periodic up‐to‐date training.ResultsOptimal control for HbA1c, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol, triglycerides and blood pressure were found in 58.6%, 51.0%, 22.4%, 53.3% and 60.2% of the population, respectively. 64.1% and 70.0% received screening for diabetic nephropathy and a dilated eye examination, respectively. When benchmarked against the HEDIS Comprehensive Diabetes Care indicators, the AUB–HIP's quality of care was 13% higher than the average of all plans in the United States. Screening for nephropathy, however, did not reach the above benchmark.ConclusionsBenchmarking served as an important tool in evaluating the current DM care offered and in detecting gaps, yet interventions are recommended for further improvement.
Semilunar Coronally Advanced Flap with or without Low Level Laser Therapy in Treatment of Human Maxillary Multiple Adjacent Facial Gingival Recessions: A Clinical Study
Authors: Singh Neelesh , Uppoor Ashita , Naik Dilip 
Published: May 2015

AbstractPurposeSemilunar coronally advanced flap (SCAF) and its modifications or adjuncts have been proposed in the literature for root coverage. Low level laser therapy (LLLT) has been shown to enhance wound healing. The aim of this split‐mouth randomized controlled clinical trial was to assess the effects of LLLT application with respect to root coverage after SCAF procedure for the treatment of human maxillary multiple adjacent facial gingival recessions.Materials and MethodsTen subjects with bilateral multiple adjacent maxillary facial gingival recession defects (Miller I and II) were included in this study (20 in test, 20 in control group). A diode laser (810 nm) at 0.3 watts was applied to test sites during and 1 week after surgery for 10 seconds. Comparisons of the surgical sites were made with clinical measurements.ResultsStatistically significant differences were observed between test and control sites in the change in gingival recession depth, gingival recession width, clinical attachment level, and width of the keratinized tissue measurements after 6 months (p = 0.003, p = 0.001, p = 0.006, and p =< 0.001, respectively). The test group presented significantly greater complete root coverage (N = 18/20, 90%) compared with the control group (N = 6/20, 30%) at 6 months post‐operatively.ConclusionWithin the confines of this study, the results depicted that a Low Level Laser Technique application may enhance the predictability of SCAF procedure. Further long‐term studies with more sample sizes are needed for a stronger evidence base.Clinical SignificanceGingival recessions are commonly encountered in dentistry and pose an esthetic concern. Minimal gingival recessions can be treated by a Semilunar Coronally Advanced Flap (SCAF), but the predictability and stability of the outcomes are quiet questionable. In the present report, a Low Level Laser Technique (LLLT) application adjunct to SCAF depicted a significant improvement in the predictability and stability of root coverage outcomes (for a period of 6 months) compared with as achieved by SCAF alone. From this report, it can be stated that LLLT may be effectively used in a day to day practice for enhancing the root coverage outcomes of SCAF.
The pharmacokinetics of pimobendan enantiomers after oral and intravenous administration of racemate pimobendan formulations in healthy dogs
Authors: Bell E. T. , Devi J. L. , Chiu S. , Zahra P. , Whittem T. 
Published: May 2015

Pimobendan is a benzimidazole‐pyridazinone derivative, marketed as a racemic mixture for the management of canine heart failure. Pharmacokinetics of the enantiomers of pimobendan and its oral bioavailability have not been described in dogs. The aim of this study was to describe pharmacokinetics of three formulations of pimobendan in healthy dogs: the licensed capsule product, and novel liquid and intravenous formulations. A three‐period, nested randomized two‐treatment crossover design was used. Pimobendan was administered p.o. at 0.25 and i.v. at 0.125 mg/kg. Blood and plasma samples were analysed by liquid chromatography–mass spectrometry. Noncompartmental modelling was used to describe the pharmacokinetics. Parameters were compared between formulations using a general linear model. Bioequivalence of the oral formulations was tested using CI90 for AUC(0–∞) and Cmax. Bioavailability of pimobendan after oral dosing was 70%. Liquid and capsule formulations were bioequivalent only for AUC. The positive enantiomer of pimobendan (PE) had a larger volume of distribution than the negative enantiomer (NE) (281 ± 48 vs. 215 ± 68 mL/kg; P = 0.003) and a shorter half‐life (21.7 vs. 29.9 min; P = 0.004). The NE was distributed more quickly than the PE into blood cells. Enantiomers of pimobendan have differing absorption, distribution and elimination. The pharmacokinetics of pimobendan in healthy dogs was described.
Pharmacokinetics of cefuroxime after intravenous, intramuscular, and subcutaneous administration to dogs
Authors: Albarellos G. A. , Montoya L. , Lorenzini P. M. , Passini S. M. , Lupi M. P. , Landoni M. F. 
Published: May 2015

Cefuroxime pharmacokinetic profile was investigated in 6 Beagle dogs after single intravenous, intramuscular, and subcutaneous administration at a dosage of 20 mg/kg. Blood samples were withdrawn at predetermined times over a 12‐h period. Cefuroxime plasma concentrations were determined by HPLC. Data were analyzed by compartmental analysis. Peak plasma concentration (Cmax), time‐to‐peak plasma concentration (Tmax), and bioavailability for the intramuscular and subcutaneous administration were (mean ± SD) 22.99 ± 7.87 μg/mL, 0.43 ± 0.20 h, and 79.70 ± 14.43% and 15.37 ± 3.07 μg/mL, 0.99 ± 0.10 h, and 77.22 ± 21.41%, respectively. Elimination half‐lives and mean residence time for the intravenous, intramuscular, and subcutaneous administration were 1.12 ± 0.19 h and 1.49 ± 0.21 h; 1.13 ± 0.13 and 1.79 ± 0.24 h; and 1.04 ± 0.23 h and 2.21 ± 0.23 h, respectively. Significant differences were found between routes for Ka, MAT, Cmax, Tmax, t½(a), and MRT. T > MIC = 50%, considering a MIC of 1 μg/mL, was 11 h for intravenous and intramuscular administration and 12 h for the subcutaneous route. When a MIC of 4 μg/mL is considered, T > MIC = 50% for intramuscular and subcutaneous administration was estimated in 8 h.
Cost implications of implementation of pathogen‐inactivated platelets
Authors: McCullough Jeffrey , Goldfinger Dennis , Gorlin Jed , Riley William J. , Sandhu Harpreet , Stowell Christopher , Ward Dawn , Clay Mary , Pulkrabek Shelley , Chrebtow Vera , Stassinopoulos Adonis 
Published: May 2015

BACKGROUNDPathogen inactivation (PI) is a new approach to blood safety that may introduce additional costs. This study identifies costs that could be eliminated, thereby mitigating the financial impact.STUDY DESIGN AND METHODSCost information was obtained from five institutions on tests and procedures (e.g., irradiation) currently performed, that could be eliminated. The impact of increased platelet (PLT) availability due to fewer testing losses, earlier entry into inventory, and fewer outdates with a 7‐day shelf life were also estimated. Additional estimates include costs associated with managing 1) special requests and 2) test results, 3) quality control and proficiency testing, 4) equipment acquisition and maintenance, 5) replacement of units lost to positive tests, 6) seasonal or geographic testing, and 7) health department interactions.RESULTSAll costs are mean values per apheresis PLT unit in USD ($/unit). The estimated test costs that could be eliminated are $71.76/unit and a decrease in transfusion reactions corresponds to $2.70/unit. Avoiding new tests (e.g., Babesia and dengue) amounts to $41.80/unit. Elimination of irradiation saves $8.50/unit, while decreased outdating with 7‐day storage can be amortized to $16.89/unit. Total potential costs saved with PI is $141.65/unit. Costs are influenced by a variety of factors specific to institutions such as testing practices and the location in which such costs are incurred and careful analysis should be performed. Additional benefits, not quantified, include retention of some currently deferred donors and scheduling flexibility due to 7‐day storage.CONCLUSIONSWhile PI implementation will result in additional costs, there are also potential offsetting cost reductions, especially after 7‐day storage licensing.
Descriptive Epidemiology of Physical Activity Levels and Patterns in New Zealanders in Advanced Age
Authors: Mace Casey , Kerse Ngaire , Maddison Ralph , Olds Timothy , Jatrana Santosh , Wham Carol , Kepa Mere , Rolleston Anna , Teh Ruth , Broad Joanna 
Published: May 2015

Background:Little is known about the physical activity levels and behaviors of advanced age New Zealanders.Methods:A cross-sectional analysis of data from Life and Living in Advanced Age: A Cohort Study in New Zealand (LiLACS NZ), Te Puāwaitanga O Nga Tapuwae Kia ora Tonu, measures of physical activity (PASE) (n = 664, aged 80–90 [n = 254, Māori, aged 82.5(2), n = 410 non-Māori, aged 85(.5)]) was conducted to determine physical activity level (PAL). A substudy (n = 45) was conducted to attain detailed information about PAL and behaviors via the Multimedia Activity Recall for Children and Adults (MARCA) and accelerometry. The main study was analyzed by sex for Māori and non-Māori.Results:Men consistently had higher levels of physical activity than women for all physical activity measures. Sex was significant for different domains of activity.
On Saying NO to People: A Pastoral Letter
Authors: Close Henry T. 
Published: May 2015

A major part of a pastor's job is to teach. He teaches people what life is like and what they can realistically expect from life. He offers his insights about what people are like, what relationships are like, what groups are like, what God is like; and suggests ways to cope with difficulties and to live a more satisfying life. This pastoral task is usually embodied in sermons. But at times the vehicle of a pastoral letter, an epistle, has been employed to teach about a Christian truth and its relevance to specific situations. Pastors in a variety of settings can find unique opportunities for this kind of teaching. The example I am offering here had its roots in a focused psychotherapy group in a mental health center. In this group patients were asked to state some specific options in living that they wanted to learn. The two most frequently stated were: (1) I want to learn to say NO to people, to be able to stand up for myself; and (2) I want to learn how to forgive myself. The following paper is addressed to the first of these concerns. It has been mimeographed and given (or read) to lots of people who have consistently commented on its helpfulness. I would suggest that papers like this can be an important part of a pastoral ministry in almost any context.
Plasma complement factor H is associated with disease activity of patients with ANCA-associated vasculitis
Authors: Chen Su-Fang , Wang Feng-Mei , Li Zhi-Ying , Yu Feng , Zhao Ming-Hui , Chen Min 
Published: May 2015

Abstract
Introduction
Increasing evidences have demonstrated that activation of alternative complement pathway plays an important role in the pathogenesis of anti-neutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV). The current study aimed to investigate the association of complement factor H (CFH), a key regulator of the alternative complement pathway, with the disease activity of AAV.

Methods
Plasma CFH levels were measured in 82 patients with myeloperoxidase (MPO)-AAV in active stage. Of the 82 patients, plasma CFH levels of 27 patients were longitudinally measured. Serum anti-CFH autoantibodies were screened in AAV patients. Circulating complement activation profiles including C4d, Bb, C3a, C5a and soluble C5b-9 of AAV patients in active stage were further detected. Associations between plasma CFH levels and clinicopathological parameters as well as the prognosis were analyzed.

Results
Plasma CFH levels were significantly lower in active AAV patients compared with AAV patients in remission and normal controls. Correlation analysis showed that plasma CFH levels inversely correlated with initial serum creatinine, Birmingham Vasculitis Activity Score (BVAS), proportion of total crescents and cellular crescents in renal specimens, and circulating levels of C3a, C5a and Sc5b-9, meanwhile positively correlated with estimated glomerular filtration rate (eGFR), hemoglobin levels and circulating levels of C3. Moreover, multivariate survival analysis revealed that plasma CFH levels were independently associated with composite outcome of death or end stage renal disease (ESRD) in AAV patients, after adjusting for age, gender, hemoglobin level and urinary protein (P = 0.03, HR 0.85, 95 % CI 0.73–0.98) or adjusting for age, gender, total crescents (%) and urinary protein (P = 0.03, HR 0.85, 95 % CI 0.73–0.98), while not as an independent predictor after adjusting for age, gender, serum creatinine and urinary protein (P = 0.57, HR 0.96, 95 % CI 0.83–1.11).

Conclusion
In conclusion, plasma CFH levels are associated with disease activity, and, to some extent, associated with composite outcomes of patients with MPO-ANCA-associated vasculitis.
Mapping hypersensitivity/allergic diseases in the International Classification of Diseases (ICD)‐11: cross‐linking terms and unmet needs
Authors: Tanno Luciana Kase , Calderon Moises , Papadopoulos Nikolaos G. , Demoly Pascal ,   EAACI/WAO Task force of a Global Classification of Hypersensitivity/Allergic diseases
Published: May 2015

AbstractBackgroundWith the aim of actively contributing to the ongoing 11th International Classification of Diseases (ICD) revision, an international collaboration led by the European Academy of Allergy and Clinical Immunology (EAACI) has decided to revise the classification of hypersensitivity/allergic diseases and to validate it for ICD‐11 by crowdsourcing the allergist community. However, understanding that the construction of a classification was necessary but not sufficient, we developed a mapping strategy in the attempt to better fit it to the ICD‐11 linearization structure.MethodsThe cross‐linking terms process has been constructed based on an algorithm in which we prioritized the pre‐coordination, followed by the post‐coordination when the first step was not possible. If the above strategies failed to identify the entries, the conditions were ruled as “non specific terms”, “no code fit properly” or “missing terms”.ResultsAmongst the 652 terms distributed in 5 main groups of the Hypersensitivity/Allergic Diseases classification, 169 terms fit directly the codes listed in the ICD‐11 beta draft (October 2014 version), 26 were considered as “nonspecific term”, 21 were linked to the Foundation by Index, 7 were recorded as inclusions and 2 were cited just in the definition of the condition. The post‐coordination was possible for 97 terms, mainly for drug hypersensitivity conditions. We noticed a considerable number of allergen references missing.ConclusionThe proposed strategy of cross‐linking terms and the results of this process can actively contribute to updating the hypersensitivity and allergic conditions classification in the ICD‐11 beta revision and underlines the need for either a new chapter in ICD‐11 possibly entitled Hypersensitivity / Allergic Disorders or at the very least the aggregation of all such diseases under the “Diseases of Immune System” chapter in order for the overlaps to be double parented to the appropriate ‘system’ chapters.
Straightforward Synthesis of HOMSi Reagents via sp2 C–H Silylation
Authors: Minami Yasunori , Komiyama Takeshi , Hiyama Tamejiro 
Published: May 2015

Abstract
Straightforward C–H silylation of heteroarenes and alkenes was found to provide efficient access to organo-HOMSi reagents as stable silicon reagents for cross-coupling. Various heteroarenes and terminal alkenes were silylated to show the versatility of the reaction. This method was applied in double silylation reactions to give bisHOMSi reagents. The silylation products could be used in the cross-coupling reaction with haloarenes easily.
Thermoresponsive Liquid Marbles Prepared with Low Melting Point Powder
Authors: Nakai Keita , Fujii Syuji , Nakamura Yoshinobu , Yusa Shin-ichi 
Published: May 2015

Abstract
Thermoresponsive liquid marbles were prepared from water and 1-bromo-3-fluoro-4-iodobenzene (BFI) powder with a melting point of 46.5 °C. The liquid marble can be floated on the surface of bulk water at room temperature, which is lower than the melting point of BFI. When the temperature of bulk water was increased above the melting point, the liquid marble disintegrated to leak the interior water to bulk water. Heating can be applied as a trigger of a chemical reaction between the interior reactant and another reactant in bulk water.
Cultivating Moina macrocopa Straus in different media using carotenogenic yeast Rhodotorula
Authors: Kushniryk Olga , Khudyi Oleksii , Khuda Lidiia , Kolman Ryszard , Marchenko Mykhailo 
Published: May 2015

Abstract
The possibility of using carotenogenic yeast from the genus Rhodotorula as food substrate was studied during the cultivation of Moina macrocopa. The results showed that replacing traditional food substrates during the cultivation of the live fish food M. macrocopa resulted in improved characteristics that were superior to the alternative. Using carotenogenic yeast Rhodotorula glutinis (Fres.) Harrison to stimulate carotenoid accumulation in live feed is better than R. rubra (Demme) Lodder. The optimal duration of enriching M. macrocopa with carotenoids was four days when using various types of yeast capable of carotenogenesis. Replacing the standard medium for culturing M. macrocopa with the alternative medium was less expensive. Breeding live feed on recirculating aquaculture system (RAS) post-production water was proven to be effective.
FE65: Roles beyond amyloid precursor protein processing
Authors: Chow Wan Ning Vanessa , Cheung Hei Nga Maggie , Li Wen , Lau Kwok-Fai 
Published: May 2015

AbstractFE65 is a brain-enriched, developmentally regulated adaptor protein that was first identified as a binding partner of amyloid precursor protein (APP), an important molecule in Alzheimer’s disease. FE65 possesses three protein interaction domains, including an N-terminal WW domain and two C-terminal phosphotyrosine-binding (PTB) domains. It is capable of mediating the assembly of multimolecular complexes. Although initial work reveals its roles in APP processing and gene transactivation, increasing evidence suggests that FE65 participates in more diverse biological processes than originally anticipated. This article discusses the role of FE65 in signal transduction during cell stress and protein turnover through the ubiquitin-proteasome system and in various neuronal processes, including neurogenesis, neuronal migration and positioning, neurite outgrowth, synapse formation and synaptic plasticity, learning, and memory.
Oncogene-dependent survival of highly transformed cancer cells under conditions of extreme centrifugal force – implications for studies on extracellular vesicles
Authors: Lee Tae Hoon , Chennakrishnaiah Shilpa , Rak Janusz 
Published: May 2015

AbstractExtracellular vesicles (EVs), including exosomes, are a subject of intense interest due to their emission by cancer cells and role in intercellular communication. Earlier reports suggested that oncogenes, such as RAS, MET or EGFR, drive cellular vesiculation. Interestingly, these oncogenes may also traffic between cells using the EV-mediated emission and uptake processes. One of the main tools in the analysis of EVs are ultracentrifugation protocols designed to efficiently separate parental cells from vesicles through a sequence of steps involving increasing g-force. Here we report that ultracentrifugationonly EV preparations from highly transformed cancer cells, driven by the overexpression of oncogenic H-ras (RAS-3) and v-src (SRC-3), may contain clonogenic cancer cells, while preparations of normal or less aggressive human cell lines are generally free from such contamination. Introduction of a filtration step eliminates clonogenic cells from the ultracentrifugate. The survival of RAS-3 and SRC-3 cells under extreme conditions of centrifugal force (110,000 g) is oncogene-induced, as EV preparations of their parental non-tumourigenic cell line (IEC-18) contain negligible numbers of clonogenic cells. Moreover, treatment of SRC-3 cells with the SRC inhibitor (PP2) markedly reduces the presence of such cells in the unfiltered ultracentrifugate. These observations enforce the notion that EV preparations require careful filtration steps, especially in the case of material produced by highly transformed cancer cell types. We also suggest that oncogenic transformation may render cells unexpectedly resistant to extreme physical forces, which may affect their biological properties in vivo.