High-Density Collagen Gel Can Repair Annular Defects and Restore Biomechanical Function to Needle-Punctured Intervertebral Discs
Authors: Grunert P. , Borde B. , Towne S. , Hudson K. , Bonassar L. , Hartl R. 
Published: May 2014

Introduction Unrepaired annular defects potentially increase the reherniation rate after lumbar discectomies and have been shown to accelerate degenerative changes after discographies. This is the first study to test an injectable biological substance to repair annular defects in vivo. We used high-density collagen gel in a needle-punctured rat-tail model. Needle puncturing leads to extrusion of NP tissue with subsequent degenerative changes (4). Restoring annular integrity will help retain the NP material and therefore inhibit these changes. Materials and Methods We punctured the S3/S4 intervertebral disc (IVD) of 30 athymic rats using an 18-gauge needle. Subsequently high-density collagen (HDC) gel was injected to seal the defect. Riboflavin (RF) was added to increase the stiffness of the collagen gel by inducing chemical cross-link formation. Animals were subdivided into four groups. The first group was injected with uncross-linked HDC gel ( n = 6), the second with cross-linked collagen using 0.5 mM ( n = 8) and the third 0.75 mM ( n = 8) concentration of RF. The fourth group ( n = 8) served as control and was left untreated after needle puncture. The animals were followed up at weeks 1, 2, 5, 12, and 18 with X-ray measurements to assess the disc heights and MR imaging to evaluate degenerative changes according to a modified Pffirmann grading system. We developed an algorithm based on T2-relaxation time measurements to assess the size of the nucleus by the number of NP voxels that compose it (Fig. 1). Animals were sacrificed at 1, 2, 5 ( n = 10), and 18 weeks ( n = 20). Histological analysis of the collected tails was performed to study the fate of the collagen using Safranin O and fluorescent stains: FITC for collagen and DAPI for host cells. Disc degeneration was assessed histologically according to the established Han grading system and NP size according to cross-sectional area measurements. Half of the tails from the 18-week time point underwent mechanical testing. We performed stress relaxation tests to measure the stiffness of the discs and their ability to pressurize. Damping quality was measured using frequency sweep tests. Explanted segments were exposed to sinusoidal strains at variable frequencies ranging from 0.01 to 0.3 Hz at amplitudes of ± 10% strain. Results Disc Degeneration Over 18 weeks RF cross-linked groups retained significantly more NP tissue in the disc space than the uncross-linked and the control groups according to NP voxel count and histological NP cross-section measurements ( p < 0.05) (Fig. 1). There was no significant difference in NP size between 0.5 and 0.75 mM groups ( p > 0.05). Both groups retained approximately 70% of NP size when compared with healthy discs and maintained a disc height of over 80% compared with the prepuncture state. Both cross-linked groups showed no significant histological degenerative changes (Fig. 1). The NP showed regular cellularity and matrix morphology. The AF maintained its organization and lamellar structure. Both groups showed similar damping qualities according to frequency sweep tests as well as a similar stiffness and ability to pressurize when compared with healthy adjacent discs. After 18 weeks, the uncross-linked collagen and the control groups showed no residual NP tissue in the disc space, and terminal degenerative changes on histological sections and according to radiological assessments (Fig. 1). MRIs showed black discs, X-rays showed disc height drop of approximately 50% (Fig. 1). Histological sections showed extruded NP tissue in the paravertebral space. The NP in the disc space was replaced by connective tissue. The IVDs of both groups were stiffer then healthy discs and showed reduced damping qualities. Fate of the Collagen At 1 week, the cross-linked collagen had formed a macroscopically visible patch sealing the defect. FITC-stained cross-linked collagen showed a tight, zipper-like adhesion to the host AF and surrounding scar tissue. At 2 weeks, the collagen gel was infiltrated with host fibroblasts which remodeled the gel into organized fibrous tissue. At 5 weeks, cross-linked collagen formed a fibrous cap which bridged the fibers of the outer anulus and thereby repaired it. This fibrous cap consisted of fibroblasts embedded in a dense fibrous matrix. This fibrous cap was still visible after 18 weeks. In the uncross-linked and control groups, there was no repair tissue visible at the outer part of the AF at any time point. The disrupted annular fibers infiltrated the surrounding scar tissue. Conclusion Annular defects induced by needle puncture do not heal over 18 weeks in the described model and lead to extrusion of nuclear tissue, with subsequent degenerative changes affecting the entire IVD. HDC is capable of repairing annular defects in a rat-tail model, thereby inhibiting these changes and improving the mechanical properties of injured discs. The repair mechanism appears to be induced by host fibroblasts which infiltrate and remodel the injected collagen gel. Annular tissue repair restored the mechanical properties of the injured discs. Although the presented results are promising, experiments in larger animals with a spinal axial load similar to humans will be necessary to evaluate true clinical potential. Disclosure of Interest None declared