bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of the bacterium Agrobacterium vitis isolated from Slovenian vineyards
Authors: FABJANČIČ Eva , Milevoj Lea 
Published: July 2024

Twenty-three bacterial isolates were isolated from fresh grapevine galls of crown gall disease of 9 different cultivars using semiselective media 3DG and RS. Eight reference strains from Germany, Hungary and Afghanistan were used as positive controls. Isolates were further characterised by means of 10 morphological, physiological and biochemical tests and their pathogenicity was checked on 4 different species of test plants. Identification and pathogenicity were also checked by the use of PCR with specific primers, which detected T-DNA carrying oncogenes acs and vis. The UPGMA dendrogram based on similarity of obtained biochemical and PCR results divideed the analysed isolates into two clearly differentiated clusters. The first cluster consisted of 18 Slovenian bacterial isolates, 3 Hungarian and 1 Afghanistan reference A. vitis strains, which show typical results for A. vitis in all biochemical tests and/or contain typical sequences of acs or vis gen. The second cluster was made up of 9 isolates, with two or more atypical results of biochemical tests and were negative in PCR. One isolate was identified as A. tumefaciens bv. 1 according to results of biochemical tests. Among 11 Slovenian and 6 foreign isolates, which were found to be pathogenic on test plants, 14 were pathogenic on tomato, 9 on Kalanchoe tubiflora and 7 isolates formed tumors on sunflower. Among 16 Slovenian A. vitis isolates identified by means of PCR 12 were octopine/nopaline and 2 were vitopin isolates.
Vpliv priprave DNK na občutljivost odkrivanja bakterij Listeria monocytogenes s PCR
Authors: KLANČNIK Anja , SMOLE MOŽINA Sonja , JERŠEK Barbara 
Published: July 2024

We studied the effect of DNA preparation methods of Listeria monocytogenes DNA on PCR sensitivity for L. monocytogenes detection with specific primers. We tested eight lysis protocols for releasing of DNA with detergents, enzymes and/or high temperatures. Considering PCR detection limit in a range from 105 to 106 cfu/ml, rapidity, low cost and preparation simplicity, we chose lysis solution consisting of 0.05 M NaOH and 0.125 % SDS to be used in the optimal procedure for L. monocytogenes DNA preparation.
Prehranski in analitski pristop k vrednotenju izkoristljivosti škroba v živilih
Authors: ŠKRABANJA Vida , GOLOB Terezija , KREFT Ivan 
Published: July 2024

In the paper a review of physiologically advantageous effects of slowly or less digested starch, passing the human gastrointestinal tract, is given. Some analytical methods for quantifying the availability of starch are described in details. Suitability, handiness and reliability of in vitro and in vivo techniques for evaluating the resistant starch content and the rate of starch hydrolysis are discussed.
Efficacy of entomopathogenic fungi for control of walnut blue butterfly (<i>Chaetoprocta odata</i> [Hewitson, 1865]) in walnut (<i>Juglans regia</i> L.) under laboratory conditions
Authors: GULL Shazia , RASOOL Ajaz , AHMAD Tariq 
Published: July 2024

Biological control nowadays is rapidly growing to reduce the incessant use of chemical insecticides for control of various insect pests. In the present study, entomopathogenic fungi are used to determine insecticidal activity against walnut blue butterfly under laboratory conditions. The experimental setup was completely randomized design (CRD) with two treatments along with control with different concentrations of entomopathogenic fungi. The bioassay was carried out by spraying second larval instar of Chaetoprocta odata [Hewitson 1865] with 1, 2, 3 & 4 % conidial concentration of Beauveria bassiana (Balsamo) Vuill. (1912) and Isaria fumosorosea Wize (1904). The results of this study showed that all the concentrations showed remarkable pathogenic activity but I. fumosorosea was highly pathogenic and recorded the highest mortality rate of 93.33 % after 144 hours compared to B. bassiana where 73.33 % mortality was reported. LC50 values for B. bassiana (4.15) was higher than that of I. fumosorosea (3.34) which indicates that I. fumosorosea was more effective against C. odata population. Among different concentrations of I. fumosorosea, 4 % concentration was the most effective with lowest LC50 values.
Study on physiological and morphological traits of purple shamrock (<i>Oxalis triangularis</i> A. St.-Hil. ) as affected by humic acid under salinity stress
Authors: ATHARI Maryam , ARGHAVANI Masoud , SOLEIMANI Ali , AELAEI Mitra , SAYYAD-AMIN Pegah , ESMAEILI Somayeh 
Published: July 2024

Salinity is one of the main environmental factors that limit plant growth and productivity. Humic acid (HA) can directly have positive effects on plant growth, and absorption of nitrogen, potassium, calcium, magnesium, and phosphorus by plants. This study aimed to concentrate on the impact of HA under salinity stress on Oxalis triangularis to use it as an ornamental plant in green spaces and landscaping. Treatments included three salinity stress levels (0, 40 and 80 mM NaCl) and four concentrations of HA (0, 200, 400, and 600 mg l-1) on O. triangularis with four replicates. Applying HA under salinity stress at 40 and 80 mM increased stem length compared to the control, but this increase was lower at medium and high salinity levels. HA at 400 mg l-1 had the best effect on rhizome length under salinity stress at 40 and 80 mM. The reduced trend on proline content in HA at 0, 200, 400, and 600 mg l-1 under salinity stress at 40 mM was 19.65, 18.45, 16.92, and 13.57 %, respectively. By increasing the concentrations of HA, anthocyanin content was raised when compared to the control. Leaf sodium and potassium decreased by applying HA with or without salinity stress.
Rhizobacteria-<i>Pseudomonas guguanensis</i> SMHMZ4 Isolated from Mines Area Assists Green-Remediation of Cadmium by <i>Brassica juncea</i>: a Promising Environment Sustainable approach
Authors: Sarita sharma Dr. Sarita Sharma 
Published: July 2024

The aim of this study was to investigate how metal-tolerant rhizobacteria isolated from the mine area and landfill site influence the phytoremediation efficacy of B. juncea plants in metal-contaminated soils. Out of four cadmium-tolerant rhizobacteria, isolate SMHMZ4 showed the promising phytoextraction efficacy of B. juncea. Isolate SMHMZ4 was identified as Pseudomonas guguanensis and submitted to NCBI GenBank under accession number MZ145097. These rhizobia were reported for the first time to support metal-Cd phytoremediation using B. juncea. Compared with the non-inoculated control, SHMMZ4 treatment significantly improved the germination of B. juncea seeds and increased soluble heavy metals in soil by 7.78 times. Growth and health parameters, pigment and metal accumulation in roots and shoots of isolate SHMMZ4 inoculated B. juncea grown in individual soil contaminated with 94.95 μg g-1 CdCl2 were significantly increased. Pot experiments showed that SHMMZ4 could transfer heavy metals from soil to roots, from roots to shoots and the values of translocation coefficient, bioconcentration coefficient, and bioaccumulation coefficient were 1.28, 1.22, and 1.72, respectively, which were significantly higher than the non-inoculated control. In the present study it is demonstrated that the amendments of rhizobacteria to B. juncea is believed to be a more promising method for green remediation of cadmium.
Load Carrying Capacity of Case-Carburized Gears with Different Cryogenic Treatments
Authors: Blech N. , Kratzer D. , Surm H. , Steinbacher M. , Tobie T. , Stahl K. 
Published: July 2024

Abstract
Gearboxes of modern drivetrains, e.g., in wind turbines, can be exposed temporarily to low ambient temperatures during their manufacturing or during operation. During manufacturing or assembly, for example, very low temperatures sometimes occur during joining processes due to the cryogenic treatment of the components. In these cases, the temperature depends on the cooling medium used (typically dry ice or liquid nitrogen). Furthermore, depending on the application, gearboxes may be exposed to low ambient temperatures up to -60 °C when used in cold regions. The low temperatures can have an impact on the component properties and thus on the gear load carrying capacity. Therefore, the knowledge of these influences is very important regarding the design and dimensioning of gearboxes.
In this publication, further investigations on the load carrying capacity of cryogenic treated gears are presented based on previous research work. The focus is on results on the tooth root and pitting load carrying capacity. The load carrying capacity is analyzed in the context of changes in the component properties as a result of different low temperature treatments, and the dominant influencing factors are identified.
Hybrid Alloyed Sinter Hardening Steels Based on Different Prealloyed Powders
Authors: Geroldinger S. , Hojati M. , de Oro Calderon R. , Gierl-Mayer C. , Danninger H. , Hellein R. 
Published: July 2024

Abstract
Sinter hardening grade steels were prepared using hybrid alloying by combining Cr-Mo and Mo prealloyed powders with fine Mn-Si masteralloy powders. Compacts from both base powders showed the “internal getter” effect, which however was much more pronounced for the Cr-Mo powders because of the markedly high starting oxygen content. Combining the base powders with the masteralloy resulted in the desired sinter hardenability, the Mo prealloyed powder resulting in somewhat better properties because of more complete dissolution of the masteralloy and lower residual oxygen content.
Biometric Technology at the Borders of Citizenship: Identifying Technical Standards for Introducer-Based Remote Onboarding in Global Contexts of Statelessness, Nomadism, Displacement, and Refuge
Authors: Vecellio Segate Riccardo 
Published: July 2024

Abstract
All throughout the so-called “Global South”, hundreds of millions of individuals from entire communities in the rural, poorer, or most peripheral areas are not officially recorded by the States they are citizens of or they habitually reside in. This is why several of such States are resorting to extensive and purportedly “universal” digital remote onboarding programs, pioneered by India’s Aadhaar, whereby individuals are centrally recorded onto a public database with their identity (and possibly citizenship) confirmed. Whenever paper documents are obsolete, inaccurate, deteriorated, or inexistent, individuals may have their identity confirmed through an “introducer”, who mediates between marginalised communities and central authorities and is entrusted by both with this delicate task. Introducers, however, cannot by themselves grant someone the status as “citizen”: they may at best confirm his or her existence and identity. These onboarding programs are enabled by wide-covering sets of technical standards, ranging from data protection and cybersecurity to interoperability, safety, disaster recovery, and business continuity. Meanwhile, similar technologies, relying on analogous standards, and fundamentally aimed at a similar purpose (that is, registering all those who fall within the prescriptive jurisdiction of a State), are deployed by border officials in the context of migration management – especially in “developed” countries. The “unofficial” and “outside-the-scope-of-the-law” components of said migratory patterns are growing exponentially due to combined effects of climate, insecurity, and geopolitical factors, increasingly originating “borderline” situations whereby identity and citizenship are challenged and contested: statelessness, refuge, nomadism (both traditional and “digital”), and internal displacement. Strikingly enough, discussions around what technical standards to adopt, and who should select them, as well as on what the role of “introducers” could be, towards the digital onboarding of individuals experiencing “borderline” configurations of citizenship are entirely neglected in socio-legal and security scholarship alike. Complemented with concrete policy proposals, the present work accepts the ambition to start bridging this gap.
Narrative as “legal tender”: the semiotic meanings of “exchange” in Malcolm Bradbury’s Rates of Exchange
Authors: Song Yanfang 
Published: July 2024

Abstract
Malcolm Bradbury’s novel Rates of Exchange, taking the economic term “rates of exchange” as a central metaphor, depicts various exchanges occurring in the socio-economic landscape of Britain during the 1970s and 1980s. In the context of governmental emphasis on economic development, the novel intricately explores exchanges among diverse entities, playing with multiple meanings of the term “exchange”. In this way, the novel itself becomes a form of “legal tender,” exchanged for reader comprehension, communication, and participation. It highlights the ubiquitous presence of exchange and the risks associated with the uncertain exchange rates between different entities. Furthermore, it invites readers to participate in the story-telling and the nostalgic journey back to British literary tradition. This paper, drawing on Roland Barthes’s semiotic theory along with structuralist and poststructuralist concepts, investigates the multifaceted meanings of “exchange” in the novel. Through this analysis, the paper aims to illuminate the semiotic significance of various narrative forms and the profound thematic concerns in Bradbury’s work.
Sox9: A potential regulator of cancer stem cells in osteosarcoma
Authors: Li Xiucheng , Lv Zuo , Zhou Ping , Zhang SongOu , Jiang Chao 
Published: July 2024

Abstract
Osteosarcoma is a highly aggressive bone tumor primarily affecting children and adolescents. Despite advancements in treatment modalities, the prognosis for osteosarcoma patients remains poor, emphasizing the need for a deeper understanding of its underlying mechanisms. In recent years, the concept of cancer stem cells (CSCs) has emerged as a crucial factor in tumor initiation, progression, and therapy resistance. These specialized subpopulations of cells possess self-renewal capacity, tumorigenic potential, and contribute to tumor heterogeneity. Sox9, a transcription factor known for its critical role in embryonic development and tissue homeostasis, has been implicated in various malignancies, including osteosarcoma. This review aims to summarize the current knowledge regarding the role of Sox9 in CSCs in osteosarcoma and its potential implications as a prognosis and therapeutic target.
Biophysical stimuli for promoting bone repair and regeneration
Authors: Bai Yunyang , Li Xiaochan , Wu Ke , Heng Boon C. , Zhang Xuehui , Deng Xuliang 
Published: July 2024

Abstract
Bone injuries and diseases are associated with profound changes in the biophysical properties of living bone tissues, particularly their electrical and mechanical properties. The biophysical properties of healthy bone are attributed to the complex network of interactions between its various cell types (i.e., osteocytes, osteoclast, immune cells and vascular endothelial cells) with the surrounding extracellular matrix (ECM) against the backdrop of a myriad of biomechanical and bioelectrical stimuli arising from daily physical activities. Understanding the pathophysiological changes in bone biophysical properties is critical to developing new therapeutic strategies and novel scaffold biomaterials for orthopedic surgery and tissue engineering, as well as provides a basis for the application of various biophysical stimuli as therapeutic agents to restore the physiological microenvironment of injured/diseased bone tissue, to facilitate its repair and regeneration. These include mechanical, electrical, magnetic, thermal and ultrasound stimuli, which will be critically examined in this review. A significant advantage of utilizing such biophysical stimuli to facilitate bone healing is that these may be applied non-invasively with minimal damage to surrounding tissues, unlike conventional orthopedic surgical procedures. Furthermore, the effects of such biophysical stimuli can be localized specifically at the bone defect site, unlike drugs or growth factors that tend to diffuse away after delivery, which may result in detrimental side effects at ectopic sites.
Development of sinter linings for high-speed trains
Authors: Bebekoğlu Sunay , Topuz Ahmet , Çakır Esin , Adsoy Abdülhamit , Yıldıran Turgay 
Published: July 2024

Abstract
In this study, brake linings with Cu (copper)-based and Cu-10Sn (bronze)-based matrix structures have been developed and produced by the powder metallurgy method using sinter metal technology for use in high-speed trains. The developed linings were compared with commercial high-speed train linings. Firstly, the mixing process was carried out in a Turbulo 3V three-dimensional mixer for 4.5 h at 50 rpm. The pressing process was performed at room temperature, under the pressure of 350–450 MPa, and the sintering temperature for Cu-based linings was 1,000 °C, while for Cu-10Sn-based linings, it was 800 °C. After the pressing and sintering processes, lining sample density calculations were conducted, and hardness tests were carried out in the Rockwell L scale. Shear strength tests, friction coefficient, and wear tests were conducted according to SAE J 661 standards. It was observed that sinter metal brake linings with Cu and Cu-10Sn main matrix structures showed similar properties and met the technical requirements. It was determined that the developed production prescriptions are promising for industrial production and patent studies.
The crystal structure of 2′-(p-tolyl)-4′H-spiro[isochromane-1,1′-naphthalene]-3,4′-dione, C25H18O3
Authors: Wang Mengdan , Li Jianyue , Zhou Yinhai , Han Zhenquan , Wang Guoda , Ma Jun-Ying 
Published: July 2024

Abstract
C25H18O3, triclinic, P1̄ (no. 2), a = 9.0717(14) Å, b = 10.2237(15) Å, c = 10.9385(18) Å, α = 101.415(4)°, β = 91.524(4)°, γ = 112.807(3)°, V = 910.7(2) Å3, Z = 2, Rgt
(F) = 0.0517, wRref
(F
2) = 0.1427, T = 293(2) K.
Gender assignment in language contact
Authors: Levkovych Nataliya 
Published: July 2024

Abstract
This paper deals with an important aspect of the integration of loan nouns into the grammatical systems of languages attesting to grammatical gender, namely gender assignment. Traditionally, it is assumed that gender assignment takes place according to the internal assignment rules of the replica language. In many cases, however, the original grammatical gender is borrowed along with the source word. This is the case of gender copy which often takes place under special (sociolinguistic) conditions and is used as assignment strategy in languages to a different extent. A special focus of my study is on gender assignment and particularly gender copy in the contact of languages of different assignment types (formal vs. semantic). The empirical data comes from five European languages in different sociolinguistic situations, attesting to different assignment systems and of different language branches of two language families – Indo-European (Romanian, Slavic, and Indo-Arian) and Nakh-Daghestanian (Lezgic and Tsezic). The analysis shows that gender copy is possible mostly in the contact of languages of the same assignment type. In the contact of languages of the formal assignment type, gender copy often goes along with the formal adjustment of the loan word. Sociolinguistic circumstances play an important role as to the possibility and frequency of the occurrence of gender copy.
Probiotics: a promising intervention for osteoporosis prevention and management
Authors: Panchal Lakshay , Arora Shivam , Pramanik Jhilam , Batta Kajol , Kumar Akash , Prajapati Bhupendra 
Published: July 2024

Abstract
Osteoporosis (OP) is a systemic skeletal disease that is characterized by low bone mass and increased fracture risk. This article explores the potential of probiotics as an adjunctive approach for the prevention and management of OP. It has been well established that the gut microbiota (GM), a complex community of microbes, plays an important role in bone health. The gut dysbiosis is linked with a higher risk of OP. However, the consumption of probiotics in adequate amounts restores gut health thus improving bone health. Probiotics may influence bone metabolism through enhanced calcium absorption, reduced inflammation, and increased bone formation. The animal and human studies demonstrate the positive effects of probiotics on bone health parameters like reduced osteoclastogenesis, bone resorption markers, osteoblast, osteocyte apoptosis, and increased bone mineral density and expression of osteoprotegerin. The current evidence suggests that probiotics can be used as an adjunctive approach along with the existing therapies for the prevention and management of OP.
KARDIOPROTEKTÍV STRATÉGIÁK A SZÍVIZOM ISZKÉMIÁS-REPERFÚZIÓS KÁROSODÁSÁVAL SZEMBEN
Authors: Oumaima El Alaoui El Abdallaoui , Komócsi András , Szokodi István 
Published: July 2024

Bevezetés: Az ST-szegment elevációval járó szívinfarktus (STEMI) előfordulása az elmúlt két évtizedben a fejlett országokban csökkenést mutatott. A reperfúziós terápiához való széles körű hozzáférés ellenére azonban a STEMI-hez kapcsolódó halálozás továbbra is jelentős.
Célkitűzés: Áttekintésünk átfogóan vizsgálja az akut szívinfarktus és a reperfúzió patofiziológiáját, felölelve az iszkémiás és reperfúziós sérülések kialakulását, a sejthalál különböző módozatait és az ebből eredő koszorúér-mikrovaszkuláris diszfunkciót. Végül részletesen tárgyaljuk a kardioprotektív gyógyszeres terápiák klinikai gyakorlatba való átültetésére irányuló erőfeszítéseket.
Eredmények: Bár a reperfúzió elengedhetetlen az iszkémiás szívizom megmentéséhez a fenyegető infarktustól, további irreverzibilis károsodást is okoz, ami az infarktus méretének növekedéséhez és mikrovaszkuláris diszfunkcióhoz vezet.
Következtetés: Az infarktus mérete és a MVO a kardioprotektív kezelés két, egymással összefüggő terápiás célpontjának tűnik. Mindazonáltal a hatékony kardioprotektív stratégiák klinikai gyakorlatban történő alkalmazása továbbra is kielégítetlen orvosi igény marad.
Síndrome de la arteria mesentérica superior en una paciente con leucemia linfoblástica aguda. Reporte de caso
Authors: Bustos-Guerrero Ada Mercedes , Alfonso-Valderrama Osman Osvaldo 
Published: July 2024

Resumen
Introducción. El síndrome de la arteria mesentérica superior, también llamado síndrome de Wilkie, ocurre por la disminución del ángulo aortomesentérico con compresión de la tercera porción duodenal. La sintomatología en muchos casos es inespecífica y su etiología se presenta por causas relacionadas con disminución de la grasa mesentérica. El diagnóstico se basa en hallazgos imagenológicos y endoscópicos. Su tratamiento se enfoca en corregir la causa relacionada con la pérdida de peso y, en casos refractarios, está indicado el manejo quirúrgico.
Presentación del caso. Mujer de 19 años que asistió a una institución de tercer nivel de complejidad en la ciudad de Bucaramanga (Colombia), por sintomatología de 2 meses consistente en pérdida de peso y pérdida parcial del apetito. Las pruebas de sangre mostraron anemia e hiperleucocitosis con 37% de blastos, por lo que se hizo dictamen de leucemia linfoide aguda diagnosticada por aspiración de médula ósea. Durante su estancia hospitalaria presentó dolor abdominal, vómito e intolerancia a la vía oral. En la tomografía de abdomen se observó distensión de la tercera y cuarta porción duodenal y una reducción del ángulo aortomesentérico (18.8º), lo que generó una obstrucción mecánica proximal del intestino. Se inició manejo médico, nutrición parenteral total y terapia postural postprandial. En imágenes de vías digestivas de control se evidenció un adecuado paso de medio de contraste hasta el yeyuno. La paciente toleró el inicio de la vía oral y presentó una buena evolución clínica.
Conclusiones. El síndrome de la arteria mesentérica superior es una causa poco frecuente de obstrucción intestinal con características clínicas variables, por lo que se requiere una alta sospecha diagnóstica. Se deben realizar estudios imagenológicos para confirmar el diagnóstico e iniciar manejo médico de forma temprana para evitar posibles intervenciones quirúrgicas.