bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nuclear translocation of β‐catenin synchronized with loss of E‐cadherin in oral epithelial dysplasia with a characteristic two‐phase appearance
Authors: Alvarado Carlos G , Maruyama Satoshi , Cheng Jun , Ida‐Yonemochi Hiroko , Kobayashi Takanori , Yamazaki Manabu , Takagi Ritsuo , Saku Takashi 
Published: October 2015

Alvarado C G, Maruyama S, Cheng J, Ida‐Yonemochi H, Kobayashi T, Yamazaki M, Takagi R & Saku T  (2011) Histopathology59, 283–291Nuclear translocation of β‐catenin synchronized with loss of E‐cadherin in oral epithelial dysplasia with a characteristic two‐phase appearanceAims:  One of the important histopathological characteristics of oral epithelial dysplasia is a two‐phase appearance of rete processes, comprising an upper layer of keratinized cells and a lower layer of basaloid cells, and thereby creating a sharp contrast between these two separate cell populations. The aim of this study was to determine the cellular adhesion status of the basaloid cells.Methods and results:  Immunohistochemistry for β‐catenin, E‐cadherin and their related molecules was carried out in surgical specimens of two‐phase epithelial dysplasia of the oral mucosa. The lower‐half basaloid cells and the upper keratinized cells were microdissected separately, and extracted DNA samples were subjected to methylation‐specific polymerase chain reaction amplification for E‐cadherin. β‐Catenin was immunolocalized within the nuclei and cytoplasm of Ki67‐positive lower‐half basaloid cells, as well as on the cell membrane of upper parakeratotic cells. The basaloid cells of the lower‐half were also positive for matrix metalloproteinase‐7 and cyclin D1, β‐catenin target gene products, α‐dystroglycan, tenascin‐C, and perlecan, but not for E‐cadherin. The promoter region of the E‐cadherin gene was hypermethylated.Conclusions:  The solid proliferation of lower‐half E‐cadherin‐free basaloid cells is enhanced by Wnt signalling cascades, as well as by the intraepithelial extracellular matrix or its bound growth factors.
Injection site pseudosarcoma in piriformis syndrome
Authors: Phan Dan C , Gleason Briana C , Fan Xuemo , Chan Owen T M , Himmelfarb Eric , Bannykh Serguei I 
Published: October 2015

Phan D C, Gleason B C, Fan X, Chan O T M, Himmelfarb E & Bannykh S I (2011) Histopathology59, 327–332Injection site pseudosarcoma in piriformis syndromeAims:  Pseudosarcomatous reactive myofibroblastic proliferations have been described following surgery or trauma at a variety of anatomical sites. These types of reactions have not been previously described at injection sites. Here we evaluated prevalence, morphologic patterns and clinical resolution of such lesions.Methods and results:  We analyzed 266 surgical resection specimens obtained during the definitive treatment of piriformis syndrome. Three cases showed exuberant reactive fibroblastic/myofibroblastic intramuscular proliferations, mimicking a sarcoma. In all three cases the surgeries were found to be preceded by local injections of cortisone and bupivacaine. Clinical follow‐up revealed no uncontrolled growth.Conclusions:  As the clinical history of injections is often not provided, it is important to be aware of this pitfall when reviewing skeletal muscle resections for entrapment syndromes.
Distribution of Zonula Occludens‐1 and Occludin and alterations of testicular morphology after in utero radiation and postnatal hyperthermia in rats
Authors: Erkanlı Şentürk Gozde , Ersoy Canillioĝlu Yasemin , Umay Cenk , Demiralp‐Eksioglu Emel , Ercan Feriha 
Published: October 2015

SummaryIn utero irradiation (IR) and postnatal hyperthermia (HT) exposure cause infertility by decreasing spermatogenic colony growth and the number of sperm in rats. Four groups were used: (i) Control group, (ii) HT group (rats exposed to hyperthermia on the 10th postnatal day), (iii) IR group (rats exposed to IR on the 17th gestational day) and (iv) IR + HT group. Three and six months after the procedures testes were examined by light and electron microscopy. Some degenerated tubules in the HT group, many vacuoles in spermatogenic cells and degenerated tight junctions in the IR group, atrophic tubules and severe degeneration of tight junctions in the IR + HT group were observed. ZO‐1 and occludin immunoreactivity were decreased and disorganized in the HT and IR groups and absent in the IR + HT group. The increase in the number of apoptotic cells was accompanied by a time‐dependent decrease in haploid, diploid and tetraploid cells in all groups. Degenerative findings were severe after 6 months in all groups. The double‐hit model may represent a Sertoli cell only model of infertility due to a decrease in spermatogenic cell and alterated blood‐testis barrier proteins in rat.
Effects of pulsed electric field processing on the quality and microbial inactivation of sour cherry juice
Authors: Altuntas Julide , Evrendilek Gulsun Akdemir , Sangun Mustafa Kemal , Zhang Howard Q. 
Published: October 2015

SummaryPulsed electric fields (PEF) processing of sour cherry juice with the measurement of pH, ºBrix, titratable acidity, conductivity, colour (L*, a* and b*), nonenzymatic browning index, metal ion concentration, total ascorbic acid and total antocyanin content as well as microbial inactivation were searched in the study. Applied PEF treatment parameters did not cause any significant difference on measured properties of sour cherry juice (P > 0.05). On the other hand, inactivation of Escherichia coli O157:H7, Staphylococcus aureus, Listeria monocytogenes, Erwinia carotowora, Pseudomonas syringae subs. syringae, Botrytis cinerea and Penicillum expansum significantly increased with increased electric field strength and treatment time (P ≤ 0.05). It was revealed that PEF is a viable option to process sour cherry juice with significant amount of microbial inactivation and without adversely affecting important physical and quality parameters.
Effect of thermal processing on oxide reductase inactivation and on colour fixing in erva‐mate (Ilex paraguariensis St. Hill) leaves
Authors: Provesi João Gustavo , Nabechima Gilson Hideki , Vieira Manoela Alano , Amante Edna Regina 
Published: October 2015

SummaryLeaves of erva‐mate (Ilex paraguariensis St. Hill) were heat treated with different combinations of time and temperature. Polyphenol oxidase (PPO) and peroxidase (POD) residual activities and colouring (L, a, b and ΔE) were studied after treatment for 3 weeks. The best results for enzyme inactivation after heat treatment were obtained with the leaves treated at 450 °C for 15 s and at 500 °C for 10 s, with reductions of 90.6% and 83.4% for PPO and 94.5% and 92.7% for POD. These groups also showed low levels of enzyme activity during the first few days of storage, and a few days later no enzyme activity was detected. The data on the colours, specially –a values (green colour), were in accordance with results for enzyme activity and helped in the research on the changes that occur in the leaves during storage. Data from this work can be suggested for the improvement of the current method of erva‐mate processing, which is still performed empirically.
Performance and fatty acid profiling of interesterified trans free bakery shortening in short dough biscuits
Authors: Handa Chanu , Goomer Sangeeta , Siddhu Anupa 
Published: October 2015

SummaryShort dough biscuits were prepared using interesterified trans free bakery shortening (IETFS). Hydrogenated shortening (HS) based biscuits were prepared as a control. The diameter, height and spread ratio were determined. Biscuits were analysed up to 8 months for hardness, moisture and acidity of the extracted fat. IETFS biscuits were significantly (P < 0.05) less hard, had a higher spread ratio and were rated higher on all sensory attributes. Their overall acceptability score on a nine point hedonic scale was 8.2 ± 0.8 as compared to 7.3 ± 0.7 for control biscuits. The fatty acid fraction of control biscuits was: saturated fat, 51.6%; trans fat, 14.5%; monounsaturated fat, 28.0%; and polyunsaturated fat, 5.9% and that of IE trans free biscuits was: saturated fat, 54.8%; trans fat, 0.6%; monounsaturated fat, 38.1%; and polyunsaturated fat, 6.5%. The IETFS was found to be appropriate as an alternate to hydrogenated shortening, providing both functionality and nutritive advantage in biscuits.
Analysis of chemical components in oolong tea in relation to perceived quality
Authors: Wang Kunbo , Liu Fang , Liu Zhonghua , Huang Jianan , Xu Zhongxi , Li Yinhua , Chen Jinhua , Gong Yushun , Yang Xinghe 
Published: October 2015

SummaryProperties of leaf and infusion colours, chemical components and volatile flavour compounds of oolong teas and their correlation with perceived quality score given by tea‐tasting panel were analysed. The scores for appearance and infused leaf correlated strongly with concentrations of chlorophyll a (chl a), chlorophyll (chl b) and chlorophyll (chl) (r = 0.355–0.433, P < 0.05) and the total quality score (TQS) positively correlated with concentrations of chl a, chl b and chl (r = 0.517–0.533, P < 0.01). The perceived taste score and TQS positively correlated with the concentration of total free amino acid (r = 0.514, 0.694, P < 0.01) and theanine (r = 0.500, 0.684, P < 0.01). The volatile composition and their quantities varied widely among oolong tea samples. Nerolidol, indole, benzeneacetaldehyde, linalool, linalool oxide I, hexanal, benzyl nitrile, geraniol and 1‐penten‐3‐ol were prevailing volatile compounds detected in most of oolong tea samples. Principal component analysis screened ten principal components with the first three (glutamic acid, total catechins and benzeneacetaldehyde) explaining 27.86%, 20.00% and 14.46% of the total variance, respectively. Regression analysis upon the ten principal components formulated a prediction model on the total quality score with 78.5% probability. The result showed that oolong teas could be partially classified by cluster analysis based on principal components.
Comparative characterisation of durian, mango and avocado
Authors: Poovarodom Sumitra , Haruenkit Ratiporn , Vearasilp Suchada , Namiesnik Jacek , Cvikrová Milena , Martincová Olga , Ezra Aviva , Suhaj Milan , Ruamsuke Pramoj , Gorinstein Shela 
Published: October 2015

SummaryThe aim of this investigation was to determine the nutritional and bioactive properties of relatively less investigated exotic fruit durian (Durio zibethinus Murr.) and to compare these indices with widely used mango (Mangifera indica L.) and avocado (Persea americana). For this purpose HPLC, three‐dimensional fluorescence (3D‐FL), several radical scavenging assays and multivariate factor analysis were used. It was found a similarity in acetone extracts between durian and mango in the contents of polyphenols (1.66 ± 0.08, 1.48 ± 0.05, mg GAE g−1 DW, respectively), and in some antioxidant assays such as ABTS (11.98 ± 0.5, 12.24 ± 0.5, μm TE g−1DW, respectively) and DPPH (5.61 ± 0.3, 5.22 ± 0.2, μm TE g−1 DW, respectively). Durian and avocado were similar in the contents of polyphenols, and ABTS and DPPH values in water and in methanol extracts, respectively. Based on the obtained results the nutritional and bioactive properties of durian are comparable with those indices in mango and avocado. In conclusion, durian can be recommended as a part of disease prevented diets.
Homeostasis and Gauss statistics: barriers to understanding natural variability
Authors: West Bruce J. 
Published: October 2015

AbstractRationale  In this paper, the concept of knowledge is argued to be the top of a three‐tiered system of science.Aims and objectives  The first tier is that of measurement and data, followed by information consisting of the patterns within the data, and ending with theory that interprets the patterns and yields knowledge. Thus, when a scientific theory ceases to be consistent with the database the knowledge based on that theory must be re‐examined and potentially modified.Results  Consequently, all knowledge, like glory, is transient.Methods  Herein we focus on the non‐normal statistics of physiologic time series and conclude that the empirical inverse power‐law statistics and long‐time correlations are inconsistent with the theoretical notion of homeostasis.Conclusions  We suggest replacing the notion of homeostasis with that of Fractal Physiology.
Music in the Park. An integrating metaphor for the emerging primary (health) care system
Authors: Sturmberg Joachim P. , Martin Carmel M. , O’Halloran Di 
Published: October 2015

AbstractBackground Metaphors are central to the human understanding of complex issues; through the immediate associations they evoke and frame problems and suggest solutions. Our suggestion ofMusic in the Parkas a metaphor for health systems reform brings to the forefront the environmentally diverse but bounded spaces of health services that offer a variety of attractors within their confines, while pushing into the background organizational and economic concerns.Reflections Parks, like health services, are embedded in their local landscape, serving their communities, but most importantly parks arepublicspaces,publicallyfunded, ideally offeringuniversal accessandequityand to besharedby all who want to go there.Music, like health, is tangible, technical and scientific, yet ultimately experiential and based on meaning. While it encompasses a wide range of styles and approaches, music making requires as its most important skill active listening which brings with it to be ‘in the moment’, to take personal risks and to draw energy and inspiration from the participants. Hence ‘audiences’ are equally active participants because music only has meaning if it internally resonates with the listener and only can exist in what is a co‐constructed experience.Conclusions  Music in the Parkis a metaphor for primary health care systems based on shared values of experts and unique local communities. Health professionals are players in this arena, who develop and practise the full range of their skills in response to individual and community needs and preferences. Their leadership works through inspiration and empowerment, making patients ‘co‐producers’ of their own health and ‘co‐shapers’ of their health services.
The knowledge, awareness, and practice patterns of dermatologists toward psychocutaneous disorders: results of a survey study
Authors: Jafferany Mohammad , Stoep Ann Vander , Dumitrescu Alin , Hornung Robin L. 
Published: October 2015

AbstractBackground  To assess the level of training in, and awareness and attitude about, psychocutaneous disorders among dermatologists.Methods  A mail‐in survey was sent to all members of Washington State Dermatology Society, who were requested to provide information on demographic variables; level of training, skills, and degree of comfort in managing psychodermatologic disorders; referral patterns, knowledge of patient and family resources on psychodermatology; and interest in continuing medical education on psychocutaneous disorders.Results  Of 237 mailed surveys, 102 were returned for analysis. Only 18% of dermatologists reported a clear understanding of psychodermatology, and 42% of the respondents reported being very comfortable in diagnosing and treating psychocutaneous disorders. Acne, atopic dermatitis and psoriasis were reported as the most common diagnoses associated with psychiatric manifestations. Delusion of parasitosis, neurotic excoriations, and trichotillomania were the most common conditions wherein patients were referred by dermatologists to psychiatrists. About 90% of the survey respondents were not aware of any patient or family resources on psychodermatology. Overall, 39% of the dermatologists expressed interest in attending any kind of continuing medical education activity on psychodermatologic disorders.Conclusion  Survey results showed that knowledge about the diagnosis, treatment and/or appropriate referral for psychocutaneous disorders is lacking. Significant information gaps were also identified in the knowledge of patient or family resources on psychocutaneous disorders. We recommend the incorporation of formal training and didactics on psychodermatology in dermatology residency programs and regular CME events. Dermatology–Psychiatry liaison services and psychodermatology clinics will prove helpful in the management of these patients in clinical settings.
Safety profile of intravenous and subcutaneous siplizumab, an anti‐CD2 monoclonal antibody, for the treatment of plaque psoriasis: results of two randomized, double‐blind, placebo‐controlled studies
Authors: Langley Richard G. , Papp Kim , Bissonnette Robert , Toth Darryl , Matheson Robert , Hultquist Micki , White Barbara 
Published: October 2015

AbstractSeveral biologics targeting different cytokines and receptors, including T‐cell receptors, have been approved for psoriasis treatment. Siplizumab, a humanized anti‐CD2 monoclonal antibody, may potentially provide an alternative therapy for psoriasis. Its safety profile and immunogenicity was examined in adults with plaque psoriasis. Two multicenter phase II randomized, double‐blind, placebo‐controlled studies: one tested two intravenous (I.V.) doses (0.012 and 0.04 mg/kg) of siplizumab every 2 weeks × 8 doses (124 patients); the second study tested three subcutaneous (S.C.) dose regimens of siplizumab (5 mg × 12 weeks, 5 mg × 6 weeks + placebo × 6 weeks, 7 mg × 4 weeks + placebo × 8 weeks), and placebo × 12 weeks (420 patients). Adverse events (AEs) and laboratory values were monitored. Immunogenicity was determined by anti‐siplizumab antibodies quantification. In both studies, siplizumab exhibited an acceptable safety profile; most common AEs judged to be siplizumab related were lymphopenia, chills, and headache, reported at a higher frequency in the siplizumab‐treated vs. placebo group. Siplizumab‐related reductions in absolute lymphocyte count did not result in clinical evidence of immune suppression. Anti‐siplizumab antibodies were detected after exposure to siplizumab; however, there was no evidence of an association between antibody development and AEs. Siplizumab exhibited an acceptable safety profile in adult patients with plaque psoriasis when administered as multiple I.V. or S.C. doses. Higher, clinically relevant doses of siplizumab would need to be tested to fully assess its safety.
Cutaneous polyarteritis nodosa: a comprehensive review
Authors: Morgan Aaron Joseph , Schwartz Robert A 
Published: October 2015

AbstractCutaneous polyarteritis nodosa is a rare form of vasculitis relating to small‐to‐medium‐sized arteries. Its etiology is unknown. Clinical manifestations include tender subcutaneous nodules, livedo reticularis, cutaneous ulcers and necrosis. Although it is distinct from systemic polyarteritris nodosa in that it lacks significant internal organ involvement, extra‐cutaneous manifestations may be evident. Commonly encountered symptoms include fever, malaise, myalgias, arthralgias, and paresthesias. Exclusion of systemic polyarteritis nodosa is essential in diagnosis. The clinical course is chronic with remissions, relapses, and a favorable prognosis. Mild cases may resolve with nonsteroidal anti‐inflammatory drugs. If more severe, treatment with systemic corticosteroids generally achieves adequate response; however, adjunctive therapy is often necessary to allow reduction in steroid dosage.
Linear growth in pediatric renal transplant recipients receiving sirolimus
Authors: Hymes Leonard C. , Warshaw Barry L. 
Published: October 2015

Hymes LC, Warshaw BL. Linear growth in pediatric renal transplant recipients receiving sirolimus. Pediatr Transplantation 2011: 15: 570–572. © 2011 John Wiley & Sons A/S.Abstract: SRL is a potent macrolide immunosuppressive agent that can be used as maintenance therapy for prevention of rejection and avoidance of CNI nephrotoxicity. However, animal studies indicate that SRL may inhibit skeletal and muscle growth. We analyzed linear growth in 25 children, age 1–15 yr old, maintained on SRL to determine whether SRL is detrimental to linear growth. Height z‐scores at baseline were compared with those at 24 months. We also compared linear growth in children receiving SRL to patients maintained on TAC. Height z‐scores over 24 months did not significantly change in the SRL group as a whole. Z‐scores improved in 13 of 25 patients (52%). Children with improved z‐scores were significantly younger than patents who did not display improved growth: 6 ± 5 yr vs. 11 ± 4 yr (p < 0.05). Height z‐scores in SRL and TAC‐based patients were no different initially and at 24 months, and a similar number of patients in each group displayed improved height scores. Height z‐scores improved in 52% of patients on SRL and occurred predominantly in younger patients for the initial 24 months of treatment. Linear growth in SRL patients was also similar to the results in TAC‐based patients. Therefore, our data did not identify a significant adverse effect of SRL on growth.
Haemostatic effect of aprotinin during craniosynostotic surgery in children
Authors: GUNNARSSON I. , HLYNSSON B. Ö. , ROSMUNDSSON TH. , THORSTEINSSON A. 
Published: October 2015

BackgroundAprotinin has been used in our hospital since the year 2003 to reduce bleeding during craniosynostotic surgery in children. The aim of this retrospective study was to investigate its effect, primarily on bleeding and secondarily on the need for transfusion.MethodsThirteen children were treated with aprotinin from 2003 to 2008, while 39 were not treated in the period 1993–2002. Information on blood loss and need for transfusion during the operations in all 52 children was collected from their medical records.ResultsThere was a significant difference in both blood loss and need for transfusion. Estimated blood volume was used to correct for difference in the children's age and size. In the aprotinin group, blood loss was 3.9% of circulating blood volume vs. 22.0%, and the need for transfusion was 0.0% vs. 21.1%.ConclusionBlood loss and need for blood transfusion were significantly reduced in the aprotinin group. No allergic or other possible aprotinin‐specific complications were registered in the aprotinin group.
Mechanisms for the Establishment of Pregnancy in the Pig
Authors: Ziecik AJ , Waclawik A , Kaczmarek MM , Blitek A , Jalali B Moza , Andronowska A 
Published: October 2015

ContentsEstablishment of pregnancy in pigs requires continuous function of corpora lutea and endometrial preparation for embryo implantation. Progesterone regulates expression of many proteins necessary for endometrial remodelling and embryo–maternal communications. Attaining the uterine receptivity involves progesterone priming and loss of progesterone receptors in the uterine epithelium before days 10–12 after oestrus. Spermatozoa and oocytes in oviduct alter secretion of specific proteins that exert beneficial effect on gametes and embryos. Moreover, an appropriate leucocyte activation and maintenance of delicate cytokine balance within the oviduct and uterus are important for early pregnancy. This early local immune response is rather mediated by seminal plasma components. These components also influence prostaglandin (PG) synthesis in the oviduct that is important for gamete and embryo transport. Pregnancy establishment requires the biphasic pattern of oestrogen secretion by conceptuses on days 11–12 and 15–30. Conceptus affects lipid signalling system consisting of prostaglandins and lysophosphatic acid. PG synthesis is changed by conceptus signals in favour of luteoprotective PGE2. Additionally, existence of PGE2 positive feedback loop in the endometrium contributes to increased PGE2/PGF2α ratio during the peri‐implantation period. PGE2 through endometrial PGE2 receptor (PTGER2) elevates the expression of enzymes involved in PGE2 synthesis. Higher PGE2 secretion in uterine lumen coincides with the elevated expression of HOXA10 transcription factor critical for implantation. A stable adhesion between conceptus and endometrium requires reduction in mucin‐1 on the apical surface of epithelium and integrin activation by extracellular matrix proteins. Furthermore, growth factors, cytokines and its receptors are involved in embryo–maternal interactions.