bluuebunny/crossref_metadata_2025_split huggingface dataset:
GLUE: 20 years on
Authors: Beven Keith , Binley Andrew 
Published: October 2013

AbstractThis paper reviews the use of the Generalized Likelihood Uncertainty Estimation (GLUE) methodology in the 20 years since the paper by Beven and Binley in Hydrological Processes in (1992), which is now one of the most highly cited papers in hydrology. The original conception, the on‐going controversy it has generated, the nature of different sources of uncertainty and the meaning of the GLUE prediction uncertainty bounds are discussed. The hydrological, rather than statistical, arguments about the nature of model and data errors and uncertainties that are the basis for GLUE are emphasized. The application of the Institute of Hydrology distributed model to the Gwy catchment at Plynlimon presented in the original paper is revisited, using a larger sample of models, a wider range of likelihood evaluations and new visualization techniques. It is concluded that there are good reasons to reject this model for that data set. This is a positive result in a research environment in that it requires improved models or data to be made available. In practice, there may be ethical issues of using outputs from models for which there is evidence for model rejection in decision making. Finally, some suggestions for what is needed in the next 20 years are provided. © 2013 The Authors.Hydrological Processespublished by John Wiley & Sons, Ltd.
Process‐based modelling of a headwater catchment in a semi‐arid area: the influence of macropore flow
Authors: van Schaik N. L. M. B. , Bronstert A. , de Jong S. M. , Jetten V. G. , van Dam J. C. , Ritsema C. J. , Schnabel S. 
Published: October 2013

AbstractSubsurface stormflow is thought to occur mainly in humid environments with steep terrains. However, in semi‐arid areas, preferential flow through macropores can also result in a significant contribution of subsurface stormflow to catchment runoff for varying catchment conditions. Most hydrological models neglect this important subsurface preferential flow. Here, we use the process‐oriented hydrological model Hillflow‐3D, which includes a macropore flow approach, to simulate rainfall‐runoff in the semi‐arid Parapuños catchment in Spain, where macropore flow was observed in previous research. The model was extended for this study to account for sorptivity under very dry soil conditions. The results of the model simulations with and without macropore flow are compared. Both model versions give reasonable results for average rainfall situations, although the approach with the macropore concept provides slightly better results. The model results for scenarios of extreme rainfall events (>13.3 mm 30 min−1) however show large differences between the versions with and without macropores. These model results compared with measured rainfall‐runoff data show that the model with the macropore concept is better. Our conclusion is that preferential flow is important in controlling surface runoff in case of specific, high intensity rainfall events. Therefore, preferential flow processes must be included in hydrological models where we know that preferential flow occurs. Hydrological process models with a less detailed process description may fit observed average events reasonably well but can result in erroneous predictions for more extreme events. Copyright © 2013 John Wiley & Sons, Ltd.
Size relationships of water discharge in rivers: scaling of discharge with catchment area, main‐stem length and precipitation
Authors: Burgers H. E. (Robin) , Schipper Aafke M. , Jan Hendriks A. 
Published: October 2013

AbstractScaling relationships between water turnover or discharge and water system size may help to reveal and understand general patterns and processes in regional and global hydrological systems. In the present study, we derived global as well as climate‐specific scaling relationships between average or maximum river discharge and catchment area, main‐stem length and precipitation, based on data from 663 monitoring stations worldwide. Data were retrieved from a Global Runoff Data Centre (GRDC) database. The scaling relationships were established with ordinary least square (OLS) and standard major axis (SMA) regressions. The focus was on the SMA regressions because this method provides better estimates of the slope.The overall empirical regressions derived were highly significant (p < 0.01). Average discharge (Q) and maximum discharge (Qmax) scaled to catchment area (A) with SMA slopes of 1.23 (r2 = 0.40) and 0.99 (r2 = 0.41), respectively. Average discharge (Q) scaled to length (L) with a slope of 2.16 (r2 = 0.40), while catchment area (A) scaled to main‐stem length (L) with a slope of 1.76 (r2 = 0.91). The addition of precipitation (P), yielding a multiple regression of discharge versus catchment area and precipitation, improved the explained variability to r2 = 0.56 and r2 = 0.52 for average and maximum discharge, respectively. Slopes of climate zone‐specific regressions tended to be similar to the slopes of the overall relationships. The uncertainties of the regressions were discussed and, where possible, compared to regressions derived in other studies. Copyright © 2013 John Wiley & Sons, Ltd.
Relative contribution of IL‐1α, IL‐1β and TNF to the host response to Mycobacterium tuberculosis and attenuated M. bovis BCG
Authors: Bourigault Marie‐Laure , Segueni Noria , Rose Stéphanie , Court Nathalie , Vacher Rachel , Vasseur Virginie , Erard François , Le Bert Marc , Garcia Irene , Iwakura Yoichiro , Jacobs Muazzam , Ryffel Bernhard , Quesniaux Valerie F. J. 
Published: October 2013

AbstractTNF and IL‐1 are major mediators involved in severe inflammatory diseases against which therapeutic neutralizing antibodies are developed. However, both TNF and IL‐1 receptor pathways are essential for the control of Mycobacterium tuberculosis infection, and it is critical to assess the respective role of IL‐1α, IL‐1β, and TNF. Using gene‐targeted mice we show that absence of both IL‐1α and IL‐1β recapitulates the uncontrolled M. tuberculosis infection with increased bacterial burden, exacerbated lung inflammation, high IFNγ, reduced IL‐23 p19 and rapid death seen in IL‐1R1‐deficient mice. However, presence of either IL‐1α or IL‐1β in single‐deficient mice is sufficient to control acute M. tuberculosis infection, with restrained bacterial burden and lung pathology, in conditions where TNF deficient mice succumbed within 4 weeks with overwhelming infection. Systemic infection by attenuated M. bovis BCG was controlled in the absence of functional IL‐1 pathway, but not in the absence of TNF. Therefore, although both IL‐1α and IL‐1β are required for a full host response to virulent M. tuberculosis, the presence of either IL‐1α or IL‐1β allows some control of acute M. tuberculosis infection, and IL‐1 pathway is dispensable for controlling M. bovis BCG acute infection. This is in sharp contrast with TNF, which is essential for host response to both attenuated and virulent mycobacteria and may have implications for anti‐inflammatory therapy with IL‐1β neutralizing antibodies.
POSTPARTUM BLUES: A MARKER OF EARLY NEONATAL ORGANIZATION?
Authors: Bydlowski Sarah , Lalanne Christophe , Golse Bernard , Vaivre‐Douret Laurence 
Published: October 2013

ABSTRACTPostpartum blues may promote a certain openness to emotional exchange between mother and her infant. Groups of mothers were assembled according to various types of emotional expression demonstrated during early postpartum. Links between mothers’ groups and newborns’ neuropsychomotor characteristics were assessed. Twenty‐one mother–infant dyads were included at maternity wards. A semistructured interview was established to identify symptoms of the blues and classify mothers into clinical groups: ordinary and emotionally mixed postpartum blues (OB); sad, intense, and lasting blues; or without blues. Newborns were examined using the Neonatal Behavioral Assessment Scale (T.B. Brazelton & J.K. Nugent, ). Cluster analysis revealed a two‐class structure, where 78% of women with OB were found to belong to the same class, with a higher median score on newborn “hand‐to‐mouth activity” which correlated to self‐quieting activity, heightened regulation of awake states, decreased need for adult support, better defensive movements, greater attentiveness, and diminished irritability. The acquired faculties demonstrated by newborns whose mothers experience OB reflect a distinct skillset of neuropsychomotor maturity.
CONSTRUCTING OPTIMAL DENSITY FORECASTS FROM POINT FORECAST COMBINATIONS
Authors: Gaglianone Wagner Piazza , Lima Luiz Renato 
Published: October 2013

SUMMARYDecision makers often observe point forecasts of the same variable computed, for instance, by commercial banks, IMF and the World Bank, but the econometric models used by such institutions are frequently unknown. This paper shows how to use the information available on point forecasts to compute optimal density forecasts. Our idea builds upon the combination of point forecasts under general loss functions and unknown forecast error distributions. We use real‐time data to forecast the density of US inflation. The results indicate that the proposed method materially improves the real‐time accuracy of density forecasts vis‐à‐vis those from the (unknown) individual econometric models. Copyright © 2013 John Wiley & Sons, Ltd.
Identification of metabolomic biomarkers for drug‐induced acute kidney injury in rats
Authors: Uehara Takeki , Horinouchi Akira , Morikawa Yuji , Tonomura Yutaka , Minami Keiichi , Ono Atsushi , Yamate Jyoji , Yamada Hiroshi , Ohno Yasuo , Urushidani Tetsuro 
Published: October 2013

ABSTRACTNephrotoxicity is a common side effect observed during both nonclinical and clinical drug development investigations. The present study aimed to identify metabolomic biomarkers that could provide early and sensitive indication of nephrotoxicity in rats. Metabolomic analyses were performed using capillary electrophoresis–time‐of‐flight mass spectrometry on rat plasma collected at 9 and 24 h after a single dose of 2‐bromoethylamine or n‐phenylanthranilic acid and at 24 h after 7 days of repeated doses of gentamicin, cyclosporine A or cisplatin. Among a total of 169 metabolites identified, 3‐methylhistidine (3‐MH), 3‐indoxyl sulfate (3‐IS) and guanidoacetate (GAA) were selected as candidate biomarkers. The biological significance and reproducibility of the observed changes were monitored over time in acute nephrotoxicity model rats treated with a single dose of cisplatin, with the glomerular filtration rate monitored by determination of creatinine clearance. Increased plasma levels of 3‐MH and 3‐IS were related to a decline in glomerular filtration due to a renal failure. In contrast, the decrease in plasma GAA, which is synthesized from arginine and glycine in the kidneys, was considered to reflect decreased production due to renal malfunction. Although definitive validation studies are required to confirm their usefulness and reliability, 3‐MH, 3‐IS and GAA may prove to be valuable plasma biomarkers for monitoring nephrotoxicity in rats. Copyright © 2013 John Wiley & Sons, Ltd.
Integrated testing and assessment approaches for skin sensitization: a commentary
Authors: Roberts David W. , Patlewicz Grace Y. 
Published: October 2013

ABSTRACTA Bayesian integrated testing strategy (ITS) approach, aiming to assess skin sensitization potency, has been presented, in which data from various types of in vitro assays are integrated and assessed in combination for their ability to predict in vivo skin sensitization data. Here we discuss this approach and compare it to our quantitative mechanistic modeling (QMM) approach based on physical organic chemistry. The main findings of the Bayesian study are consistent with our chemistry‐based approach and our previously published assessment of the key determinants of sensitization potency, in particular the relatively high predictive value found for chemical reactivity data and the relatively low predictive value for bioavailability parameters. As it stands at present the Bayesian approach does not utilize the full range of predictive capability that is already available, and aims only to assign potency categories rather than numerical potency values per se. In contrast, for many chemicals the QMM approach can already provide numerical potency predictions. However, the Bayesian approach may have potential for those chemicals where a chemistry modeling approach cannot provide a complete answer (e.g. pro‐electrophiles whose in cutaneo activation cannot currently be modeled confidently). Nonetheless, our main message is of the importance of leveraging chemistry insights and read‐across approaches to the fullest extent possible. Copyright © 2013 John Wiley & Sons, Ltd.
Rate control of cell sheet recovery by incorporating hydrophilic pattern in thermoresponsive cell culture dish
Authors: Kumashiro Yoshikazu , Matsunaga Teruyuki , Muraoka Megumi , Tanaka Nobuyuki , Itoga Kazuyoshi , Kobayashi Jun , Tomiyama Yumiko , Kuroda Masatoshi , Shimizu Tatsuya , Hashimoto Iwao , Umemura Kazuo , Yamato Masayuki , Okano Teruo 
Published: October 2013

AbstractThready stripe‐polyacrylamide (PAAm) pattern was fabricated on a thermoresponsive poly(N‐isopropylacrylamide) (PIPAAm) surface, and their surface properties were characterized. A PIPAAm surface spin‐coated with positive photoresist was irradiated through a 5 µm/5 µm or a 10 µm/10‐µm black and white striped photomask, resulting in the radical polymerization of AAm on the photoirradiated area. After staining with Alexa488 bovine serum albumin, the stripe‐patterned surface was clearly observed and the patterned surface was also observed by a phase contrast image of an atomic force microscope. NIH‐3T3 (3T3) single cells were able to be cultured at 37°C on the patterned surfaces as well as on a PIPAAm surface without pattern, and the detachment of adhered cells was more rapidly from the patterned surface after reducing temperature. Furthermore, the rate of detachment of 3T3 confluent cell sheet on the patterned surface was accelerated, compared with on a conventional PIPAAm surface under the static condition. The rate control of cell sheet recovery should contribute the preservations of cell phenotype and biological functions of cell sheet for applying to clinical trials. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2849–2856, 2014.
A novel peptide‐modified and gene‐activated biomimetic bone matrix accelerating bone regeneration
Authors: Pan Haitao , Zheng Qixin , Yang Shuhua , Guo Xiaodong , Wu Bin , Zou Zhenwei , Duan Zhixia 
Published: October 2013

AbstractThe osteogenic differentiation of bone marrow stromal cells (BMSCs) can be regulated by systemic or local growth factor, especially by transforming growth factor beta 1 (TGF‐β1). However, how to maintain the bioactivity of exogenous TGF‐β1 is a great challenge due to its short half‐life time. The most promising solution is to transfer TGF‐β1 gene into seed cells through transgenic technology and then transgenic cells to continuously secret endogenous TGF‐β1 protein via gene expression. In this study, a novel non‐viral vector (K)16GRGDSPC was chemically linked to bioactive bone matrices PLGA‐[ASP‐PEG]n using cross‐linker to construct a novel non‐viral gene transfer system. TGF‐β1 gene was incubated with this system and subsequently rabbit‐derived BMSCs were co‐cultured with this gene‐activated PLGA‐[ASP‐PEG]n, while co‐cultured with PLGA‐[ASP‐PEG]n modified with (K)16GRGDSPC only and original PLGA‐[ASP‐PEG]n as control. Thus we fabricated three kinds of composites: Group A (BMSCs‐TGF‐β1DNA‐(K)16GRGDSPC‐PLGA‐[ASP‐PEG]n composite); Group B (BMSCs‐(K)16GRGDSPC‐PLGA‐[ASP‐PEG]n composite); and Group C (BMSCs‐PLGA‐[ASP‐PEG]n composite). TGF‐β1 and other osteogenic phenotype markers of alkaline phosphatase, osteocalcin, osteopontin and type I collagen in Group A were all significantly higher than the other two groups ex vivo. In vivo, 15‐mm long segmental rabbit bone defects were created and randomly implanted the aforementioned composites separately, and then fixed with plate‐screws. The results demonstrated that the implants in Group A significantly accelerated bone regeneration compared with the other implants based on X‐rays, histological and biomechanical examinations. Therefore, we conclude this novel peptide‐modified and gene‐activated biomimetic bone matrix of TGF‐β1DNA‐(K)16GRGDSPC‐PLGA‐[ASP‐PEG]n is a very promising scaffold biomaterial for accelerating bone regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2864–2874, 2014.
Structural and physicochemical aspects of silica encapsulated ZnO quantum dots with high quantum yield and their natural uptake in HeLa cells
Authors: Depan D. , Misra R. D. K. 
Published: October 2013

AbstractPhotoluminescent semiconductor quantum dots (QDs) are of significant interest for bioimaging and fluorescence labeling. In this regard, we describe here the design of high sensitivity and high specificity non‐toxic ZnO QDs (∼5 nm) with long‐term stability of up to 12 months. The embedding of ZnO QDs on silica nanospheres led to significant increase in photoluminescence intensity rendering them highly bright QD‐based probes. The QDs were characterized in vitro with respect to cancer cells (HeLa) and evaluated in terms of viability, fluorescence and cytoskeletal organization. The immobilization of ZnO QDs on silica nanospheres promoted the internalization and enhanced fluorescence emission of HeLa cells. The fluorescence emission from QDs was stable for 3 days, indicating excellent stability toward photobleaching. Cytoskeletal reorganization was observed after internalization of QDs such that the ZnO QDS on silica nanospheres resulted in broadening of the actin cytoskeleton. The study underscores that ZnO QDs immobilized on Si nanospheres are promising for tracking cancer cells in cell therapy. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2934–2941, 2014.
Reticulated bioactive scaffolds with improved textural properties for bone tissue engineering: Nanostructured surfaces and porosity
Authors: Ramiro‐Gutiérrez M. Lourdes , Will Julia , Boccaccini Aldo R. , Díaz‐Cuenca Aránzazu 
Published: October 2013

AbstractOrganised nanoporous SBA‐15 type silica precursor (SP) particulate material has been processed into three‐dimensional macroporous, reticulated structures using a novel strategy consisting of blending increasing percentages of SP with a SiO2‐CaO‐P2O5 (80Si15Ca5P) mesoporous bioactive glass (MBG) sol. The procedure successfully produced consolidated and functionally competent open‐cell scaffolds while preserving the nanoporous order of the SP. Scaffolds were prepared using four different (MBG)/(SP) ratios. These structures were then characterized using field emission gun scanning electron microscopy, X‐ray diffraction (XRD), nitrogen adsorption‐desorption measurements, and compressive strength testing. Open‐cell interconnected structures with dual macro (150–500 μm) and nano (4–6 nm)‐organised porosity were produced. Both the textural and mechanical properties were found to improve with increasing SBA‐15 content. The in vitro bioactive response using simulated body fluid confirmed high reactivity for all prepared scaffolds. In addition, the SBA‐15 containing scaffolds exhibited a superior ability to delay the pH‐triggered lysozyme release with antibiotic activity. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2982–2992, 2014.
Surface‐modified functionalized polycaprolactone scaffolds for bone repair: In vitro and in vivo experiments
Authors: Jensen Jonas , Rölfing Jan Hendrik Duedal , Svend Le Dang Quang , Kristiansen Asger Albæk , Nygaard Jens Vinge , Hokland Lea Bjerre , Bendtsen Michael , Kassem Moustapha , Lysdahl Helle , Bünger Cody Eric 
Published: October 2013

AbstractA porcine calvaria defect study was carried out to investigate the bone repair potential of three‐dimensional (3D)‐printed poly‐ε‐caprolactone (PCL) scaffolds embedded with nanoporous PCL. A microscopic grid network was created by rapid prototyping making a 3D‐fused deposition model (FDM‐PCL). Afterward, the FDM‐PCL scaffolds were infused with a mixture of PCL, water, and 1,4‐dioxane and underwent a thermal‐induced phase separation (TIPS) followed by lyophilization. The TIPS process lead to a nanoporous structure shielded by the printed microstructure (NSP‐PCL). Sixteen Landrace pigs were divided into two groups with 8 and 12 weeks follow‐up, respectively. A total of six nonpenetrating holes were drilled in the calvaria of each animal. The size of the cylindrical defects was h 10 mm and Ø 10 mm. The defects were distributed randomly using following groups: (a) NSP‐PCL scaffold, (b) FDM‐PCL scaffold, (c) autograft, (d) empty defect, (a1) NSP‐PCL scaffold + autologous mononuclear cells, and (a2) NSP‐PCL scaffold + bone morphogenetic protein 2. Bone volume to total volume was analyzed using microcomputed tomography (µCT) and histomorphometry. The µCT and histological data showed significantly less bone formation in the NSP‐PCL scaffolds in all three variations after both 8 and 12 weeks compared to all other groups. The positive autograft control had significantly higher new bone formation compared to all other groups except the FDM‐PCL when analyzed using histomorphometry. The NSP‐PCL scaffolds were heavily infiltrated with foreign body giant cells suggesting an inflammatory response and perhaps active resorption of the scaffold material. The unmodified FDM‐PCL scaffold showed good osteoconductivity and osseointegration after both 8 and 12 weeks. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2993–3003, 2014.
Toll‐like receptors‐2 and 4 are overexpressed in an experimental model of particle‐induced osteolysis
Authors: Valladares Roberto D. , Nich Christophe , Zwingenberger Stefan , Li Chenguang , Swank Katherine R. , Gibon Emmanuel , Rao Allison J. , Yao Zhenyu , Goodman Stuart B. 
Published: October 2013

AbstractAseptic loosening secondary to particle‐associated periprosthetic osteolysis remains a major cause of failure of total joint replacements (TJR) in the mid‐ and long term. As sentinels of the innate immune system, macrophages are central to the recognition and initiation of the inflammatory cascade, which results in the activation of bone resorbing osteoclasts. Toll‐like receptors (TLRs) are involved in the recognition of pathogen‐associated molecular patterns and danger‐associated molecular patterns. Experimentally, polymethylmethacrylate and polyethylene (PE) particles have been shown to activate macrophages via the TLR pathway. The specific TLRs involved in PE particle‐induced osteolysis remain largely unknown. We hypothesized that TLR‐2, ‐4, and ‐9 mediated responses play a critical role in the development of PE wear particle‐induced osteolysis in the murine calvarium model. To test this hypothesis, we first demonstrated that PE particles caused observable osteolysis, visible by microCT and bone histomorphometry when the particles were applied to the calvarium of C57BL/6 mice. The number of TRAP positive osteoclasts was significantly greater in the PE‐treated group when compared to the control group without particles. Finally, using immunohistochemistry, TLR‐2 and TLR‐4 were highly expressed in PE particle‐induced osteolytic lesions, whereas TLR‐9 was downregulated. TLR‐2 and ‐4 may represent novel therapeutic targets for prevention of wear particle‐induced osteolysis and accompanying TJR failure. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3004–3011, 2014.