bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pemodelan Regresi Global (Glm) dan Regresi Spasial (Sar Dan Sdm) Pada Kasus Indeks Pembangunan Manusia Di Provinsi Kalimantan Selatan
Authors: Marliana Ana 
Published: August 2024

The Human Development Index (HDI) is a parameter that functions to assess the success of the quality of human life. The factors used in the research are the severity of poverty, population density and net participation rate. The research carried out aims to see what factors influence the HDI in South Kalimantan Province in 2022, the HDI value for South Kalimantan Province is below the Indonesian HDI value and quite a few regencies/cities in South Kalimantan Province have HDI values below the HDI value. Indonesia. The statistical analysis used is a spatial approach, where the SAR and HR spatial regression models will be searched. The Global Regression Model (GLM) obtained in this study is , while the Spatial Autoregressive (SAR) model is and Spatial Durbin Model is The best model that can be obtained is the Spatial Durbin Model (SDM) with an AIC value of 52,82654 and an value of 95,86%.
QUICK ROBUST CLUSTERING USING LINKS (QROCK) UNTUK PENGELOMPOKAN DESA KABUPATEN BANJAR
Authors: Shofari Muhammad Rizki 
Published: August 2024

AbstractAccurate village profile planning needs to be done with mapping based on its characteristics. Village grouping based on the characteristics of village facilities and potential or its characteristics based on the Building Village Index indicator can help determine priorities in village development. In this study, mixed data was used, with numerical data grouping using Hierarchical Agglomerative Nesting (AGNES) algorithm and categorical data with Quick Robust Clustering Using Links (QROCK). The resulting clusters are then combined using the QROCK Ensemble algorithm (algCEBMDC). The data is sourced from the 2021 Village Potential Data Collection (PODES) by the Central Statistics Agency in 277 villages in Banjar Regency, including 18 numerical variables and 29 categorical variables. The results of the study obtained optimal clusters based on the ratio of within-group standard deviation (SW) to between-group standard deviation (SB) resulting in a ratio of 4.82.10-9 with a threshold of 0.4 to 0.9 resulting in 6 clusters. The best cluster results are cluster 4 (4 villages) and cluster 3 (14 villages), then cluster 2 (villages) and cluster 1 (186 villages), and clusters that need development priorities are cluser 5 (2 villages) and cluster 6 (1 village) which are outliers based on processing results. Keywords: Village Grouping, Cluster, AGNES Algorithm, Quick Robust Clustering Using Links (QROCK), AlgCEBMDC
Amending high sugar in V. vinifera cv. Shiraz wine must by pre-fermentation water treatments results in subtle sensory differences for naïve wine consumers
Authors: Xynas Billy , Barnes Christopher , Howell Kate 
Published: August 2024

Global warming presents challenges to all wine-grape growers and winemakers across the globe. Increasing growing season temperatures means that grapes are harvested with higher sugar concentrations than preferred for high-quality winemaking. The now Australian permitted water-treatment of high-sugar musts can produce wines with lower alcohol levels, however, recent studies show impacts on associated chemical compounds and sensory parameters can affect final wine quality. Commercial applications based on these earlier findings are limited as many focus on one vintage, one or two harvest timings, small wine samples and limited numbers of panellists for sensory analysis. We expand by first investigating the chemical profiles and sensory parameters of wines produced from V. vinifera cv. Shiraz for three harvest times: early 13.5°Baumé (°Bé), middle 14.5 °Bé and late 15.5 °Bé across two vintages (2020/2021). Just-About-Right sensory analysis assesses 159 naïve wine consumer panellists’ feedback. We show late 15.5 °Bé and middle 14.5 °Bé wine has the highest levels for chemical compounds of colour density (a.u.), total tannin (%), total pigment (a.u.), total phenolics (a.u.) and free anthocyanins (mg/L), as confirmed by PCA analysis outcomes. Sensory analysis shows the least preference for vegetal smell (2.50) in 13.5 °Bé wine, with the highest preference for colour intensity (3.06) in early 13.5 °Bé wine and body in late 15.5 °Bé wine. Secondly, 15.5 °Bé and middle 14.5 °Bé pre-fermentation musts are subject to water dilution or ‘bleed and replace’ treatments. These wines have lower final alcohol levels, decreases in most chemical compound levels, but with subtler effects for sensory profiles. For instance, there is a high preference for astringency (3.01), ripe fruit taste (2.96) and hotness (2.95) in late diluted 13.5 °Bé wine. Vegetal smell (2.89) is most preferred in late ‘bleed and replace’ 13.5 °Bé wine. All water treatments have minimal impact on dark fruit smell, ripe fruit smell, odour intensity, odour complexity, red fruit taste and dark fruit taste. Middle diluted 13.5 °Bé wine has the highest overall rating and is the most preferred. This study offers valuable insights revealing that water treatments of pre-fermentation musts can produce wines with preferable lower alcohol levels, and while this winemaking approach may negatively affect several chemical compounds, many favourable sensory properties can still be preserved. This is a crucial consideration for winemakers when addressing wine quality from these winemaking techniques.
Inflectional forms in studentsâ€™ writing: A point of view from morphological analysis
Authors: Amuntyastuti Sekar , Sriati Usman  , Bochari Siska 
Published: August 2024

The objective of this study was to determine the most difficult and dominant inflectional form in students' writing as well as the variables that affect how difficult it is for students to comprehend the function of inflectional forms. This study examined inflectional markings in regular and irregular forms of content words. The design of this study was quantitative descriptive with a population taken from the 3rd-semester students of the English Education Study Program of Tadulako University who have enrolled Grammar subject. The sample of this study was 154 students of the 3rd-semesterÂ students by using a simple random sampling technique. The instruments to collect the data were a test and a questionnaire. The results of the data analysis show that: (1) the most difficult form of inflectional forms used by the students was suffix -est as superlative degree, (2) the most dominant form of inflectional forms used by the students was the suffix -ing as progressive marker, (3) there were two factors influencing the studentsâ€™ difficulty in using the function of inflectional forms which were internal factors. The test results indicate that students lack sufficient knowledge of tenses, as they are unable to apply the present, past, or past participle tenses.
Amino acids catalyze RNA formation under ambient alkaline conditions
Authors: Braun Dieter , Rout Saroj , Wunnava Sreekar , Powner Matthew , Šponer Judit , Mast Christof 
Published: August 2024

Abstract

RNA and proteins are the core molecules of life. The sequence of mRNA encodes the protein sequence and proteins orchestrate the replication of nucleic acids. How this synergistic relationship arose remains an unanswered question. Amino acids have not yet provided an evolutionary advantage to nucleic acid synthesis. This relationship has been assumed as a late development during RNA evolution. Here we show that amino acids substantially improve the yield of RNA polymerization from prebiotically plausible ribonucleoside-2',3'-cyclic phosphates. The amino acid catalysis of RNA polymerization was base-selective, increased the compositional oligomer sequence diversity and favoured the formation of natural 3',5' linkages. It was most efficient at pH values close to the pKaH of the amines and correlated with sidechain hydrophobicities, suggesting a general acid-base mechanism akin to ribozyme-catalysed RNA-hydrolysis. This is confirmed by the observed general base-enhanced polymerization of G and U nucleotides. The copolymerization of G/C/A/U with valine resulted in a substantially increased oligomer yield and compositional diversity. The catalytic effect of proteinogenic amino acids on RNA oligomerization suggests the reactivity of amino acids and RNA was united earlier than previously thought, and their association likely predates the genetic code.
Prototypical innate immune mechanism hijacked by leukemia-initiating mutant stem cells for selective advantage and immune evasion in Ptpn11-associated juvenile myelomonocytic leukemia
Authors: Zheng Hong , Zhao Peng , Tan Zhenya , Yu Wen-Mei , Werner Juwita , Stieglitz Elliot , Porter Chris , Chandrakasan Shanmuganathan , Wechsler Daniel , Mendez-Ferrer Simon , Qu Cheng-Kui 
Published: August 2024

Abstract

Juvenile myelomonocytic leukemia (JMML), a clonal hematologic malignancy, originates from mutated hematopoietic stem cells (HSCs). The mechanism sustaining the persistence of mutant stem cells, leading to leukemia development, remains elusive. In this study, we conducted comprehensive examination of gene expression profiles, transcriptional factor regulons, and cell compositions/interactions throughout various stages of tumor cell development in Ptpn11 mutation-associated JMML. Our analyses revealed that leukemia-initiating Ptpn11E76K/+ mutant stem cells exhibited de novo activation of the myeloid transcriptional program and aberrant developmental trajectories. These mutant stem cells displayed significantly elevated expression of innate immunity-associated anti-microbial peptides and pro-inflammatory proteins, particularly S100a9 and S100a8. Biological experiments confirmed that S100a9/S100a8 conferred a selective advantage to the leukemia-initiating cells through autocrine effects and facilitated immune evasion by recruiting and promoting immune suppressive myeloid-derived suppressor cells (MDSCs) in the microenvironment. Importantly, pharmacological inhibition of S100a9/S100a8 signaling effectively impeded leukemia development from Ptpn11E76K/+ mutant stem cells. These findings collectively suggest that JMML tumor-initiating cells exploit evolutionarily conserved innate immune and inflammatory mechanisms to establish clonal dominance.
Provably secure multilayers certificateless proxy signature for Industrial Internet of Things
Authors: Ullah Rafiq , Mehmood Amjad , Khan Muhammad Altaf , Ullah Insaf 
Published: August 2024

Abstract

The emergent nature of Industrial Internet of Things (IIoTs) raises significant authentication concerns in Certificateless Proxy Signature (CLPS) to ensure protection from modern forgery attacks. Due to some modern forgery attacks, single layer authentication is unable to provide secure CLPS with optimal computational cost and communication overhead in IIoTs. Therefore, multilayer authentication is required to secure the identities of the participants on open channel using Zero-Knowledge Proof (ZKP) mechanism. It offers a promising opportunity for addressing authentication vulnerabilities in IIoTs. This article proposed an optimal and secure multilayer authentication technique with minimum computational cost and communication overhead. The proposed mechanism evolved CLPS with zero knowledge proof using Schnorr signature in a transparent way, Whereas, the security hardness od the proposed work is proofed informally with Hyperelliptic Curve (HEC). Moreover, for formal verification, AVISPA tool is used. Performance analysis depicts that the proposed approach exhibits not only lower computational cost and communication overhead but also provides optimal security as compared to existing approaches.
The complete genome assembly of Nicotiana benthamiana reveals genetic and epigenetic landscape of centromeres
Authors: Guo Li , Chen Weikai , Yan Ming , Chen Shaoying , Sun Jie , Wang Jingxuan , Meng Dian , Li Jun , Zhang Lili 
Published: August 2024

Abstract

Nicotiana benthamiana is a model organism widely adopted in plant biology and biotechnology. Its genomic research has lagged since its initial release in 2012. To further improve its usefulness, we generate and phase the complete 2.85 Gb genome assembly of allotetraploid N. benthamiana with all 19 centromeres and 38 telomeres fully resolved. We find that although Solanaceaecentromeres are widely dominated by Ty3/Gypsy retrotransposons, satellite-based centromeres are surprisingly common in N. benthamiana with 11 of 19 centromeres featured by megabase-scale satellite arrays. Interestingly, the satellite-enriched and satellite-free centromeres are extensively invaded by distinct Gypsy retrotransposons where CENH3 protein more preferentially occupies, suggestive of their crucial roles in centromere function. We demonstrate that rDNA is a major origin of centromeric satellites, and mitochondrial DNA could be employed as core component of centromere. Subgenome analysis indicate emergence of satellite arrays likely drives the centromere formation and maturation during genomic shock after polyploidization. Altogether, we propose N. benthamiana centromeres evolves via neocentromere formation, satellite expansion, retrotransposon enrichment, and mtDNA integration.
PRSS55 Regulates Energy Homeostasis and BCAA Metabolism via Interacting with BCKDK in Mouse Testes and Sperm
Authors: Ge Haoyang , Shen Chunling , Xiong Wenfeng , Lu Shunyuan , Li Chaojie , Tang Lingyun , Shen Yan , Zhang Hongxin , Fei Jian , Wang Zhugang 
Published: August 2024

Abstract

Previous studies have shown that PRSS55 is required for male fertility in mice, and potentially in humans, and is involved in energy metabolism in mouse sperm. However, the underline mechanism remains unclear. Here we show that Prss55 deficiency leads to significant changes in protein levels identified by DIA-based mass spectrometry proteomics in enriched PRSS55-expressing testicular cells and caudal epididymal sperm as compared to wt mice. Bioinformatic assessments reveal that the majority of differentially expressed proteins (DEPs) were significantly enriched in the branched-chain amino acids (BCAA) degradation pathway and the oxidative phosphorylation (OXPHOS) process. Accumulation of isoleucine and leucine was identified in Prss55-/- mice by untargeted metabolomics. We also observe significantly downregulated IVD and NDUFB8 protein levels, impaired in adenosine triphosphate (ATP) production, abnormal NAD+/NADH ratio, and decreased mitochondrial membrane potential in knockout models. Secondly, co-immunoprecipitation/mass spectrometry (Co-IP/MS) by using V5-PRSS55 knock-in mice and in vitro Co-IP assays suggested that PRSS55 interacts with mitochondrial protein BCKDK, which is involved in BCAA metabolism. Overexpressed PRSS55 was found to be enriched in mitochondria by immunofluorescence and immunoblotting assays. All these data demonstrate that PRSS55 regulates energy homeostasis and BCAAs metabolism in testes and sperm of mice, implicating a potential mechanism of male infertility in mice lacking PRSS55 and in human due to PRSS55 mutation.
Calix-like molecules with outstanding optical properties designed based on the Aza- BODIPY molecule: a DFT study
Authors: Yarahmadi Mahvash , Shamlouei Hamid Reza , Afshari Sadegh 
Published: August 2024

Abstract

Using aza-BODIPY chromophores, structurally and thermodynamically stable calix-like oligomers have been designed and studied. By performing optimization calculations, it was found that firstly, all the structures do not have imaginary frequency; secondly, they have high cohesive energy, which is a proof of the stability of the structures. In order to achieve the materials with enhanced color and improved optical properties, the H and F atoms from the upper edge of the calix structures was removed and the resonance of system was enlarge to decrease the Eg of the systems. By removing the H and F atoms from the upper edge of calix molecules, the maximum of absorption peaks were transferred to the visible area, and the intensity of light-color properties in the structures were greatly increased. Especially since the size of the calixes increases, the optical properties are enhanced; So that the most absorption is related to calixes 6, 7, 8 and 9. The calix[8] -aza-BODIPY(with removed H atom from each unit CH2) oligomer was detected as the most colored molecule with absorption coefficient ε = 300,000, f= 2.0459, λ = 441.03.
Effects and mechanism of APPL1 on proliferation of gastric cancer cells
Authors: Zhai Junshan , Zhu Jianhua , Wang Qi , Yang Mi , Zhang Jing , Wei Huaizhi , Wu Kai 
Published: August 2024

Abstract

BACKGROUND: APPL1 is highly expressed in gastric cancer tissues and it is positively correlated with TNM stage, depth of invasion, and lymph node metastasis of gastric cancer, but the specific mechanism of APPL1 on gastric cancer cell proliferation has not been defined. In our study, we prepared the gastric carcinoma cell line SGC-7901/APPL1 via plasmid transfection technique, then investigated the change of gastric carcinoma cells' proliferation ability, aimed to investigate the regulatory mechanism of APPL1 on gastric cancer cell proliferation.
METHODS The gastric carcinoma cell strain SGC-7901 was transfected by APPL1 gene plasmid as the experimental group; by blank plasmid as the control group. The expression of APPL1 on the experimental group was detected by Western-blot technique. The change of the experimental group cells ' proliferation ability was detected by MTT assay, The change of the experimental group cell cycle was detected by PI staining, The change of the experimental group cell cyclin proteins were detected by Western-blot technique.
RESULTS By the Western- blot technique, the expression level of APPL1 protein in the experimental group cell strain was up-regulated effectively(P<0.01). Meanwhile, the proliferation ability of the experimental group increased significantly was detected by MTT assay(P<0.05), By the PI staining, the percentage of the experimental group cell cycle in G1 phase was decreased(P<0.01), in S phase was increased(P<0.01). By the Western-bolt technique, the expression of Cyclin D1(P<0.05) was up-regulated(P<0.05) and p16, p27 was down-regulated(P<0.05), Cyclin E、p21、p57 were not changed(P>0.05).
CONCLUSION Through the plasmid transfection technique, the gastric carcinoma cell line that could overexpress APPL1 protein were prepared successfully and whose proliferation ability were enhanced.
Advanced Catalytic Syntheses of 1,3-Oxazine Derivatives via Electrophilic Substitution Using Mono- and Bimetallic Phosphate Catalysts: Co₃(PO 4 ) 2 and CoCa 3 (HPO 4 )(PO 4 ) 2 (OH)
Authors: hallaoui Achraf El , FERRAA Nouhaila , MERROUN Yousssef , CHEHAB Soukaina , BOUKHRIS Said , Ziatni Mounia Bennani , SOUIZI Abdelaziz , GHAILANE Rachida 
Published: August 2024

Abstract

This research introduces a novel catalytic approach for the synthesis of 1,3-oxazinone derivatives through a multicomponent reaction that relies on an electrophilic substitution mechanism, employing two cobalt-based phosphate catalysts. This approach, allowed us to synthesize oxazine derivatives, known for their significant biological activities, achieving impressive yields of up to 97% in a remarkably short time of only 7 minutes, all under conditions environmentally friendly according to the principles of green chemistry using the ethanol/water mixture as a green solvent. In our study, we utilized two catalysts derived from cobalt-modified phosphate, which were synthesized in our laboratory using simple methods. The first support consists of a monometallic catalyst, denoted as Co3(PO4)2, whereas the second is a bimetallic phosphate catalyst modified with cobalt and calcium CoCa3(HPO4)(PO4)2(OH). Our developed methods had various advantages including simplicity of the process, rapid reaction time, simple clean-up, ability to recover and reuse the catalyst and overall simplicity. All these advantages render this developed approach effective and viable for synthesizing oxazine derivatives suitable for large-scale applications.
Exploring Behavioral and Psychological Influences on Weight Regain and Weigh Loss After 3 Years Undergone Bariatric Surgery: In-Depth Exploration
Authors: Althumiri Nora A. , BinDhim Nasser F. , Al-Rayes Saja A. , Alumran Arwa 
Published: August 2024

Abstract

Background: Bariatric surgery is often regarded as the gold standard treatment for obesity. However, the long-term success of this intervention depends not only on the procedure itself but also on subsequent behavioral and psychological adaptations.
Objective:This study aimed to in-depth explore the behavioral and psychological factors influencing weight gain or loss among participants three years post-bariatric surgery.
Methods: Employing a mixed-methods approach, the study combined descriptive quantitative and interpretative qualitative methodologies to achieve a comprehensive analysis. Data were collected through questionnaires for quantitative insights and in-depth interviews for qualitative exploration. This methodological integration allowed for a thorough examination of a wide range of variables affecting post-surgical weight management.
Results: The findings revealed a wide variance in the percentage of weight regain among participants, ranging up to 89.0% of the lowest weight achieved post-surgery. Almost half of the participants with obesity perceive themselves as overweight rather than with obesity. The majority of participants did not change their lifestyle after the surgery. Seventy-five percent are not following any nutritional or dieting programs, and only less than 12% engage in any physical activity, with a higher level of sedentary lifestyle.
Conclusion: These results revealed the complex interplay of factors that influence weight management after surgical intervention. The recurrence of obesity among the participants was primarily attributed not to the surgical procedures themselves but to insufficient postoperative behavioral changes, underscoring the necessity for integrated support systems focusing on lifestyle modifications and psychological support to enhance long-term surgical outcomes.