bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sandwich freezing and freeze substitution of Arabidopsis plant tissues for electron microscopy
Authors: Yamaguchi Masashi , Sato Mayuko , Takahashi-Nakaguchi Azusa , Okamoto Michiyo , Toyooka Kiminori , Chibana Hiroji 
Published: July 2024

Abstract
Sandwich freezing is a method of rapid freezing by sandwiching specimens between two copper disks, and it has been used for observing exquisite close-to-native ultrastructure of living yeast and bacteria. Recently, this method has been found to be useful for preserving cell images of glutaraldehyde-fixed cultured cells, as well as animal and human tissues. In the present study, this method was applied to observe the fine structure of living Arabidopsis plant tissues and was found to achieve excellent ultrastructural preservation of cells and tissues. This is the first report of applying the sandwich freezing method to observe plant tissues.
Therapeutic plasma exchange for hyperviscosity syndrome in IgA multiple myeloma
Authors: Yurtsever Nalan , Binns Thomas C , Hendrickson Jeanne E , Tormey Christopher A , Lee Edward S 
Published: July 2024

Abstract
Hyperviscosity syndrome (HVS) is defined as the symptomatic presentation of increased blood thickness due to various clinical conditions such as hypergammaglobulinemia. HVS secondary to immunoglobulin (Ig)A multiple myeloma has been infrequently reported. Although the efficiency of IgM or IgG removal by therapeutic plasma exchange (TPE) is well described, the efficiency of IgA removal by TPE is not as well known. Here, we describe a case of HVS due to IgA myeloma in a patient who received 2 TPE treatments, with subsequent symptomatic improvement as well as decrease in IgA and viscosity levels.
Halo densities and pericenter distances of the bright Milky Way satellites as a test of dark matter physics
Authors: Andrade Kevin E , Kaplinghat Manoj , Valli Mauro 
Published: July 2024

ABSTRACT
We provide new constraints on the dark matter halo density profile of Milky Way (MW) dwarf spheroidal galaxies (dSphs) using the phase-space distribution function (DF) method. After assessing the systematics of the approach against mock data from the Gaia Challenge project, we apply the DF analysis to the entire kinematic sample of well-measured MW dwarf satellites for the first time. Contrary to previous findings for some of these objects, we find that the DF analysis yields results consistent with the standard Jeans analysis. In particular, in this study we rediscover (i) a large diversity in the inner halo densities of dSphs (bracketed by Draco and Fornax), and (ii) an anticorrelation between inner halo density and pericenter distance of the bright MW satellites. Regardless of the strength of the anticorrelation, we find that the distribution of these satellites in density versus pericenter space is inconsistent with the results of the high-resolution N-body simulations that include a disc potential. Our analysis motivates further studies on the role of internal feedback and dark matter microphysics in these dSphs.
Why does the Milky Way have a metallicity floor?
Authors: Smith Britton D , O’Shea Brian W , Khochfar Sadegh , Turk Matthew J , Wise John H , Norman Michael L 
Published: July 2024

ABSTRACT
The prevalence of light element enhancement in the most metal-poor stars is potentially an indication that the Milky Way has a metallicity floor for star formation around $\sim 10^{-3.5}$ Z$_{\odot }$. We propose that this metallicity floor has its origins in metal-enriched star formation in the minihaloes present during the Galaxy’s initial formation. To arrive at this conclusion, we analyse a cosmological radiation hydrodynamics simulation that follows the concurrent evolution of multiple Population III star-forming minihaloes. The main driver for the central gas within minihaloes is the steady increase in hydrostatic pressure as the haloes grow. We incorporate this insight into a hybrid one-zone model that switches between pressure-confined and modified free-fall modes to evolve the gas density with time according to the ratio of the free-fall and sound-crossing time-scales. This model is able to accurately reproduce the density and chemo-thermal evolution of the gas in each of the simulated minihaloes up to the point of runaway collapse. We then use this model to investigate how the gas responds to the absence of H$_{2}$. Without metals, the central gas becomes increasingly stable against collapse as it grows to the atomic cooling limit. When metals are present in the halo at a level of $\sim 10^{-3.7}$ Z$_{\odot }$, however, the gas is able to achieve gravitational instability while still in the minihalo regime. Thus, we conclude that the Galaxy’s metallicity floor is set by the balance within minihaloes of gas-phase metal cooling and the radiation background associated with its early formation environment.
Randomized Controlled Trial of a Text-Based Smokeless Tobacco Cessation Intervention for Rural and Medically Underserved Communities
Authors: Noonan Devon , Silva Susan G , Fish Laura J , Simmons Leigh Ann , Nwankwo Nneze , Scherr Karen , Da Costa Mariana , Sang Elaine , Sanders Camila , Swinkels Courtney , Garcia Ortiz Norma , Severson Herbert H , Pollak Kathryn I 
Published: July 2024

Abstract

Introduction
Smokeless tobacco use remains prevalent in rural and medically underserved populations, leading to increased rates of tobacco-related cancers and chronic disease. While access to effective cessation programs is limited, text-based interventions may offer a delivery approach with broad reach. This two-armed randomized control trial (RCT) assessed the efficacy of #EnufSnuff.TXT, a text-based smokeless tobacco cessation intervention, in rural and medically underserved communities.

Aims and Methods
We conducted a two-arm RCT assessing #EnufSnuff.TXT, a text-based scheduled reduction intervention paired with text-based cessation support messages compared with the modified Enough Snuff intervention comprised of a cessation education booklet and bi-weekly motivational text messages. We recruited participants via social media and surveyed participants at 3 and 6 months post-randomization. The primary outcome was self-reported 7-day point prevalence abstinence at 6 months.

Results
We recruited and randomized 532 participants. At 3 months post-randomization, the quit rate was significantly higher in #EnufSnuff.TXT arm compared to the Enough Snuff arm for intent-to-treat (ITT) cases (29.2% vs. 19.0%, odd ratios [OR] = 1.75, p = .0066). The quit rate at 6 months post-randomization remained higher in #EnufSNuff.TXT compared to Enough Snuff for ITT cases (23.1% vs. 20.9%, OR = 1.14, p = .5384), although no longer significantly different.

Conclusions
This is the first large-scale text-based cessation clinical trial for individuals in underserved areas who use smokeless tobacco. The #EnufSnuff.TXT intervention performed better in the short term; however, both interventions yielded similar quit rates at 6 months post-randomization. Future research should focus on improving long-term abstinence in the #EnufSNuff.TXT intervention.

Implications
Text-based cessation approaches have the potential to increase access to cessation interventions in rural and medically underserved areas and reduce tobacco-related chronic disease morbidity and mortality. Our study shows short-term efficacy from the first-ever randomized controlled trial of a smokeless tobacco cessation intervention, #EnufSnuff.TXT, for rural and medically underserved residents in the United States. Our #EnufSnuff.TXT Intervention offers a scalable solution to reach and provide much-needed access to cessation interventions in medically underserved, rural communities in the United States. This work provides the foundation for further inquiry on augmented text-based approaches to increase cessation in this at-risk group.

Clinical Trials Registration
A text-based Reduction Intervention for Smokeless Tobacco Cessation, NCT04315506.
A review of mitotane in the management of adrenocortical cancer
Authors: Del Rivero Jaydira , Else Tobias , Hallanger-Johnson Julie , Kiseljak-Vassiliades Katja , Raj Nitya , Reidy-Lagunes Diane , Srinivas Sandy , Gilbert Jill , Vaidya Anand , Aboujaoude Emily , Bancos Irina , Tito Fojo Antonio 
Published: July 2024

Abstract

Importance
Mitotane (Lysodren, o,p'-DDD [1-(o-chlorophenyl)-1-(p-chlorophenyl)-2,2-dichloroethane)] is currently the only United States Food and Drug Administration and European Medicines Agency-approved product for the treatment of adrenocortical carcinoma.

Observations
Mitotane is challenging to administer; however, its toxicities (specifically adrenal insufficiency) are well known, and the management of adverse consequences has established approaches. While often viewed through the prism of a cytotoxic agent, it can also interfere with hormone production making it a valuable asset in managing functional ACC. A recently completed prospective trial has shed some light on its use in the adjuvant setting, but further clarity is needed. Many think mitotane has a role in the advanced or metastatic setting, although prospective data are lacking and retrospective analyses are often difficult to interpret.

Conclusions and relevance
When used carefully and thoughtfully, especially in patients with hormonal excess, mitotane is an important component of the treatment armamentarium for ACC.
The Influence of Active, Passive, and Manual Therapy Interventions on Escalation of Health Care Events After Physical Therapist Care in Veterans With Low Back Pain
Authors: Mayer John M , Highsmith Michael Jason , Maikos Jason , Patterson Charity G , Kakyomya Joseph , Smith Bridget , Shenoy Nigel , Dearth Christopher L , Farrokhi Shawn 
Published: July 2024

Abstract

Objective
The objective of this study was to examine the associations between active, passive, and manual therapy interventions with the escalation-of-care events following physical therapist care for veterans with low back pain (LBP).

Methods
A retrospective cohort study was conducted in 3618 veterans who received physical therapist care for LBP between January 1, 2015 and January 1, 2018. The Department of Veterans Affairs (VA) Corporate Data Warehouse was utilized to identify LBP-related physical therapist visits and procedures, as well as opioid prescription and non–physical therapy clinic encounters. The association between physical therapist interventions with 1-year escalation-of-care events were assessed using adjusted odds ratios from logistic regression.

Results
Nearly all veterans (98%) received active interventions, but only a minority (31%) received manual therapy. In the 1-year follow-up period, the odds of receiving an opioid prescription were 30% lower for those who received manual therapy in addition to active interventions, as compared with patients who received only active interventions. Moreover, the odds of receiving primary care, specialty care, and diagnostic testing were 30% to 130% higher for patients who received electrical stimulation or more than 1 passive intervention in addition to active treatments, as compared with patients who received only active interventions.

Conclusion
The use of manual therapy along with active interventions was associated with reduced prescription of opioids, while utilization of specific passive interventions such as electrical stimulation or multiple modalities in conjunction with active interventions resulted in increased escalation-of-care events.

Impact
The use of active interventions, which is supported by most clinical practice guidelines, was the cornerstone of physical therapist care for veterans with LBP. However, the use of clinical practice guideline–recommended manual therapy interventions was low but associated with reduced opioid prescriptions. The use of 2 or more different passive interventions along with active interventions was common (34%) and associated with less-than-optimal escalation-of-care outcomes.
The Prediction of Diagnostic Change From Bipolar Disorder to Schizophrenia and Schizophrenia to Bipolar Disorder in a Population-Based, Longitudinal, National Swedish Sample
Authors: Kendler Kenneth S , Abrahamsson Linda , Sundquist Jan , Sundquist Kristina 
Published: July 2024

Abstract

Background and Hypothesis
To clarify, in a large, representative, longitudinal sample, the rate and predictors of diagnostic conversion from Bipolar Disorder (BD) to Schizophrenia (SZ) and from SZ→BD.

Design
From individuals born in Sweden 1950–1995 and living there in 1970 or later, we identified at least one initial diagnoses of SZ (n = 8449) and BD (n = 8438) followed for a minimum of 10 and a mean of 24 years. Diagnostic conversion required, respectively, at least two final diagnoses of BD and SZ 30 days apart with no intervening diagnosis of SZ or BD.

Results
At follow-up, rates of BD→SZ and SZ→BD conversion were 10.1 and 4.5%, respectively. Conversions occurred slowly, with around 50% completed in the first decade. Using a diverse range of variables available at first onset including family genetic risk scores, BD→SZ conversion was predicted with greater accuracy (AUC = 0.78) than SZ→BD conversion (AUC = 0.65). The strongest predictors of BD→SZ conversion were earlier years of birth, younger age at BD onset, low BD genetic risk, and being unmarried at BD onset. SZ→BD conversion was most strongly predicted by high BD genetic risk, being married at SZ onset, female sex, early age at SZ onset, and an MD episode prior to SZ onset. Cases of BD and SZ in the highest decile for conversion risk had HRs for a diagnostic change of, respectively, 12.5 and 3.4.

Conclusions
Diagnostic conversion of BD→SZ and SZ→BD are not rare, are moderately predictable, and should likely be accounted for in many research designs.
Clozapine Safety in Pregnancy: A Clinical Study
Authors: Kulkarni Jayashri , De Chellis Adam , Gilbert Heather , Gavrilidis Emmy , Mu Eveline , Karimi Leila , Li Qi 
Published: July 2024

Abstract

Background and Hypothesis
Pregnant women with persistent schizophrenia and related disorders may require ongoing antipsychotic treatment, including clozapine. However, the potential risks of using clozapine during pregnancy and the postnatal period remain uncertain.

Study Design
We conducted a nested case-control study using the National Register of Antipsychotic Medication in Pregnancy (NRAMP) database. Our study assessed pregnancy outcomes among Australian women diagnosed with schizophrenia spectrum disorder and treated with clozapine (n = 14) during the first trimester. These women were compared to 2 subgroups: those treated with quetiapine (n = 53) and those not taking any medication (n = 24) during pregnancy.

Study Results
We observed higher rates of miscarriage in the clozapine group compared to the quetiapine and drug-free groups. The clozapine group had a higher early pregnancy body mass index but lower overall pregnancy weight gain than the other groups. The prevalence of gestational diabetes was significantly higher in the clozapine group. The percentage of vaginal delivery was higher in the clozapine group than in the other 2 groups. Neonatal outcomes such as gestational age, and Apgar scores were similar across groups. The birth weight was lower in the clozapine group compared to the other 2 groups.

Conclusions
This study suggests that pregnant women taking clozapine and their babies have greater adverse outcomes compared to other groups. Clozapine appears to be associated with a greater risk of miscarriages, maternal gestational diabetes, and lower birth weight. However, the gestational age, Apgar scores, and admission to NICU/SCN were comparable between all groups.
Ethical Challenges in Social Work Licensing Examinations: A Call for Integrity and Strategies for Success
Authors: Joseph Rigaud 
Published: July 2024

Abstract
This article discusses four questions. First, what is the operational framework of the Association of Social Work Boards (ASWB)? Second, how congruent is the ASWB’s operational framework with social work values and ethics? Third, why do ASWB exam candidates from disadvantaged backgrounds—those who identify as African American, who are older, and who do not speak English as a first language—register comparatively lower pass rates in the clinical licensure examination? Fourth, what are ethically sound novel strategies for improving the ASWB exam? This article argues that (1) ASWB, in its present state, operates under a business model philosophy, which is incongruent with the social work value of integrity, and (2) the disparities in pass rates in the ASWB exam may reflect its lack of validity. This article also presents three relatively innovative strategies for restoring confidence and increasing success in the ASWB social work licensing exam and two contingent solutions to the problem of exam pass rate disparities based on race, age, and primary language. The implications of this study for social work stakeholders are also discussed.
Evaluating Patient Perceptions of Smartphone Use for Active and Passive Collection of Health Data
Authors: O’Neill Colin , Duckworth Elizabeth , Shah Romil , Jayakumar Prakash 
Published: July 2024

Background:
The objectives of this study are to assess patient willingness to share smartphone generated health data with providers and to characterize patient populations who express hesitation around providing these data to providers.

Methods:
Bivariate analysis involved unpaired t-test or analysis of variance for comparing continuous variables. An alpha value of 0.05 was used. A multivariable regression to investigate demographic factors associated with comfort with sharing health data was performed. Odds ratios compared participants by age group, educational attainment, and race.

Results:
A total of 485 participants completed the survey, with an average age of 38 years old. Respondents were 65% male and 73% Caucasian. The majority (70%) of participants were comfortable answering questions about their health on their phone, as well as sending pictures of their health problems or wounds. Over half of participants were comfortable with passive social and physical activity information being sent to their doctor and would allow their doctors to download a data tracking application on their smartphone (55% and 59%, respectively). Younger participants (OR 2.0, P<0.05) and participants with increased education (OR 2.5, P < 0.01) were more likely to be comfortable in sharing their data. Privacy concerns were cited as the most common reason patients desired to refrain from sharing data.

Conclusion:
Most study participants were comfortable sharing smartphone generated health data.
Current and future trends of acupuncture as an adjuvant therapy in cancer: A bibliometric and visual analysis
Authors: Shang Rui , Pan Ting , Wang Fengyang , Jin Hui , Nan Xi , Song Chenyu 
Published: July 2024

Background:
Acupuncture, as one of the representatives of complementary therapies, is often used as an adjunctive therapy in the treatment of cancer and related complications with beneficial results.

Methods:
We selected the Web of Science Core Collection (WOSCC) database as the data source and searched to obtain relevant literatures on the application of acupuncture in cancer treatment. The relevant literature was analyzed using CiteSpace (version 6.1.R6) and VOSviewer (version 1.6.20).

Results:
A total of 592 publications were included, with an overall upward trend in the number of publications. There were 135 countries or institutions involved, with China as the most published country. There were 1888 related institutions, and Memorial Sloan Kettering Cancer Center was the institution with the most publications. A total of 3067 authors were involved, and there were obvious collaborations between authors. There were 190 related journals, and J Clin Oncol was the most cited journal. The most frequently occurring keywords were breast cancer and management, but also other related topics such as quality of life, chemotherapy, and complementary.

Conclusion:
Acupuncture is gaining increasing attention as an adjunctive therapy in cancer treatment programs. Currently, relevant research focuses on acupuncture to alleviate cancer-induced comorbid symptoms, such as pain and insomnia. Future research is gradually shifting toward spiritual care, Colorectal Cancer Surgery, and systematic review.
Research progress of airway inflammation in asthma: A bibliometric analysis
Authors: Liye Lang , Hui Zhao , Fuchun Huang , Hua Liu 
Published: July 2024

Background:
In recent years, the prevalence of asthma has gradually increased and the number of asthmatics worldwide has reached 358 million, which has caused huge economic loss. Airway inflammation is an important feature of asthma, and international research in this field has a high degree of heat. Therefore, this paper uses the bibliometric method to systematically review and visualize the literature in this field, aiming to provide some reference value for follow-up related research.

Methods:
To retrieve the research literature on airway inflammation in asthma from 2003 to 2022 in the Web of Science Core Collection database. The bibliometric method was used to systematically analyze the included literature data by using visualization analysis software such as CiteSpace (6.2. R4) and VOSviewer (1.6.19).

Results:
A total of 1892 articles published in 423 journals were included in this study, from 1912 institutions in 62 countries/regions. The number of articles published between 2003 and 2022 showed a trend of fluctuating growth. The country with the largest number of articles published was China (558,29.49 %), followed by the United States (371,19.61 %) and Korea (212,11.21 %). Gibson, Peter G is the author with the highest number of publications, and Journal of Allergy and Clinical Immunology is the most published journal.

Conclusion subsections:
This study systematically reveals the state of the literature in the field of airway inflammation in asthma over the past 20 years. The exploration of inflammatory cell components, pathway molecules and biological agents are research hotspots in this field and should be further studied.
Multimodal imaging analysis of autosomal recessive bestrophinopathy: Case series
Authors: Miura Masahiro , Makita Shuichi , Yasuno Yoshiaki , Azuma Shinnosuke , Mino Toshihiro , Hayashi Takaaki , Kameya Shuhei , Tsunoda Kazushige 
Published: July 2024

Rationale:
Autosomal recessive bestrophinopathy (ARB) is a subtype of bestrophinopathy caused by biallelic mutations of the BEST1 gene, which affect the retinal pigment epithelium (RPE). Studying RPE abnormalities through imaging is essential for understanding ARB. This case series involved the use of multimodal imaging techniques, namely autofluorescence (AF) imaging at 488 nm [short-wavelength AF] and 785 nm [near-infrared AF (NIR-AF)] and polarization-sensitive optical coherence tomography (PS-OCT), to investigate RPE changes in 2 siblings with ARB.

Patient concerns:
Two Japanese siblings (Case 1: male, followed for 20–23 years; Case 2: female, followed for 13–17 years) carried compound heterozygous mutations of the BEST1 gene.

Diagnosis:
Both siblings were diagnosed with ARB.

Interventions and outcomes:
Multimodal imaging techniques were used to evaluate RPE changes. Both siblings had funduscopic changes similar to those seen in the vitelliruptive stage of Best vitelliform macular dystrophy during the follow-up period. NIR-AF imaging showed hypo-AF of the entire macular lesion in both cases, and this hypo-AF remained stable over time. PS-OCT confirmed reduced RPE melanin content in these hypo-AF areas. Additionally, hyper-NIR-AF dots were observed within hypo-NIR-AF areas. Concomitant identification of focally thickened RPE melanin on PS-OCT imaging and hyper-AF on short-wavelength AF imaging at the sites containing hyper-NIR-AF dots indicated that the hyper-NIR-AF dots had originated from either stacked RPE cells or RPE dysmorphia.

Lessons:
We confirmed RPE abnormalities in ARB, including diffuse RPE melanin damage in the macula alongside evidence of RPE activity-related changes. This case series demonstrates that multimodal imaging, particularly NIR-AF and PS-OCT, provides detailed insights into RPE alterations in ARB.