bluuebunny/crossref_metadata_2025_split huggingface dataset:
Controlling Localization and Excretion of Nanoparticles by Click Modification of the Surface Chemical Structures inside Living Cells
Authors: Ito Takeo , Nakamura Takuma , Kusaka Eriko , Kurihara Ryohsuke , Tanabe Kazuhito 
Published: April 2015

AbstractInvited for this month’s cover is the group of Drs. Takeo Ito and Kazuhito Tanabe from Kyoto University, Japan. The cover image shows accumulation of silica nanoparticles on the plasma membrane by click modification of the surface in living cells. Read the full text of the article at 10.1002/cplu.201402436.
Melt‐Polymerization of TEMPO Methacrylates with Nano Carbons Enables Superior Battery Materials
Authors: Vlad Alexandru , Rolland Julien , Hauffman Guillaume , Ernould Bruno , Gohy Jean‐François 
Published: April 2015

AbstractA solvent‐free, melt polymerization process of a 2,2,6,6‐tetramethylpiperidine‐1‐oxyl (TEMPO) precursor for rechargeable organic radical batteries is proposed. In situ carbon incorporation in the melted monomer phase yields a nanoscale homogenous polymer composite. Superior battery performances including higher power and cycling stability are attained by using the melt‐polymerization method.
Soil water depletion and recharge under different land cover in China's Loess Plateau
Authors: Fan Jun , Wang Quanjiu , Jones Scott B. , Shao Mingan 
Published: April 2015

AbstractVegetation restoration can greatly influence water infiltration and root water uptake depths in semi‐arid regions. Soil water dynamics within different vegetated/non‐vegetated land cover patterns were studied over time from 2008 to 2013 in an erodible small watershed on the Loess Plateau of China. Results showed that distinct differences in soil water dynamics and water balances existed among different vegetation types. Soil water recharge depths extended deeper than 5 m under bare land and farmland, but introduced forage (alfalfa) and shrub (caragana) species depleted the soil water stores in the 0‐ to 6‐m layers within a few years, prevented deep soil water recharge and exhibited negative water balances. Local food crops can maintain a sustainable soil water balance although there was low productivity. Native grasses should be the best land use type for this region based on the soil water balances observed in this short field study. Because it was difficult to avoid soil desiccation under introduced vegetation, vegetation with high water demands should be combined with shallow rooting plants, and water‐saving methods should be taken into account. Copyright © 2015 John Wiley & Sons, Ltd.
Copper(I)‐Catalyzed A3‐Coupling Based Reaction of Alkynes, Aldehydes, and Amines: An Efficient Approach to 2,5‐Polysubstituted Dihydrofurans and 2,5‐Disubstituted Furans
Authors: Fan Wu , Ma Shengming 
Published: April 2015

AbstractAn efficient and practical A3‐coupling based reaction that gives a variety of 2,5‐polysubstituted dihydrofurans and 2,5‐disubstituted furan derivatives has been developed. This domino copper‐catalyzed three‐component coupling reaction of propargylic alcohol, ethyl glyoxalate, and an amine affords a propargylic amine intermediate, which undergoes an alkyne–allene isomerization followed by a cyclization to yield the dihydrofuran. Upon treatment with CH3I and base, the latter was smoothly converted into a 2,5‐disubstituted furan. Alternatively, the furan derivatives can be directly prepared by a two‐step reaction sequence.
Reduced memory skills and increased hair cortisol levels in recent Ecstasy/MDMA users: significant but independent neurocognitive and neurohormonal deficits
Authors: Downey Luke A. , Sands Helen , Jones Lewis , Clow Angela , Evans Phil , Stalder Tobias , Parrott Andrew C. 
Published: April 2015

ObjectivesThe goals of this study were to measure the neurocognitive performance of recent users of recreational Ecstasy and investigate whether it was associated with the stress hormone cortisol.MethodsThe 101 participants included 27 recent light users of Ecstasy (one to four times in the last 3 months), 23 recent heavier Ecstasy users (five or more times) and 51 non‐users. Rivermead paragraph recall provided an objective measure for immediate and delayed recall. The prospective and retrospective memory questionnaire provided a subjective index of memory deficits. Cortisol levels were taken from near‐scalp 3‐month hair samples.ResultsCortisol was significantly raised in recent heavy Ecstasy users compared with controls, whereas hair cortisol in light Ecstasy users was not raised. Both Ecstasy groups were significantly impaired on the Rivermead delayed word recall, and both groups reported significantly more retrospective and prospective memory problems. Stepwise regression confirmed that lifetime Ecstasy predicted the extent of these memory deficits.ConclusionsRecreational Ecstasy is associated with increased levels of the bio‐energetic stress hormone cortisol and significant memory impairments. No significant relationship between cortisol and the cognitive deficits was observed. Ecstasy users did display evidence of a metacognitive deficit, with the strength of the correlations between objective and subjective memory performances being significantly lower in the Ecstasy users. Copyright © 2015 John Wiley & Sons, Ltd.
A Glimpse into the Future: Cross‐border Judicial Cooperation in Insolvency Cases in the European Union
Authors: Wessels Bob 
Published: April 2015

AbstractAfter 2 years of study, discussion and consultation, in February 2015, the EU Cross‐Border Insolvency Court‐to‐Court Cooperation Principles were published. The EU Cross‐Border Insolvency Court‐to‐Court Cooperation Principles (‘EU JudgeCo Principles’) contain 26 principles. The EU JudgeCo Principles aim to strengthen efficient and effective communication between courts in EU Member States in insolvency cases with cross‐border effects. The EU JudgeCo Principles, in short, include principles on their non‐binding status and their objectives, case management of courts and the equal treatment of creditors, and principles about the judicial decisions itself, on the reasoning and for instance on providing a stay or moratorium. Several principles relate to the course of the proceedings, such as notifications and authentication of documents, and the last principles concern the outcome of judicial cooperation, for instance, cross‐border sales, assistance to a reorganisation or rules for binding creditors to an international reorganisation plan. The Principles include 18 EU Cross‐Border Insolvency Court‐to‐Court Communications Guidelines (‘EU JudgeCo Guidelines’). These EU JudgeCo Guidelines aim to facilitate communications in practice, in individual cross‐border cases. The EU JudgeCo Principles try to overcome present obstacles for courts in EU Member States such as formalistic and detailed national procedural law, concerns about a judge's impartiality, uneasiness with the use of certain legal concepts and terms, and, evidently, language. Presently, court‐to‐court communication between judges in insolvency matters in the EU, especially on the continent, is limited to only a few cases. In the near future, judicial cooperation and communication will be a cornerstone in the efficient and effective administration of insolvency cases within the EU. The EU JudgeCo Principles will then certainly serve as a significant guide. Copyright © 2015 INSOL International and John Wiley & Sons, Ltd
Interspecies and Interkingdom Signaling via Quorum Signals
Authors: Clinton Allie , Rumbaugh Kendra P. 
Published: April 2015

AbstractQuorum sensing (QS) is an evolutionarily conserved mechanism of cell‐to‐cell communication utilized by hundreds of different species of microbes. QS is mediated by small molecules and their corresponding receptors, many of which are similar in structure and function. Consequently, microbes have developed ways to detect signals produced by their prokaryotic and eukaryotic neighbors and alter their behavior accordingly. These interactions have been termed interspecies and interkingdom signaling, respectively. In most described cases, bacteria use this sensory information to either up‐regulate their own virulence or down‐regulate the virulence of their neighbor. In this review, we describe many of the known interspecies and interkingdom signaling events that are mediated by quorum signals, and discuss the impact these may have on the ability of microbes to interact with each other and with their hosts.
Involvement of Different networks in mammary gland involution after the pregnancy/lactation cycle: Implications in breast cancer
Authors: Zaragozá Rosa , García‐Trevijano Elena R. , Lluch Ana , Ribas Gloria , Viña Juan R. 
Published: April 2015

AbstractEarly pregnancy is associated with a reduction in a woman's lifetime risk for breast cancer. However, different studies have demonstrated an increase in breast cancer risk in the years immediately following pregnancy. Early and long‐term risk is even higher if the mother age is above 35 years at the time of first parity. The proinflammatory microenvironment within the mammary gland after pregnancy renders an “ideal niche” for oncogenic events. Signaling pathways involved in programmed cell death and tissue remodeling during involution are also activated in breast cancer. Herein, the major signaling pathways involved in mammary gland involution, signal transducer and activator of transcription (STAT3), nuclear factor‐kappa B (NF‐κB), transforming growth factor beta (TGFβ), and retinoid acid receptors (RARs)/retinoid X receptors (RXRs), are reviewed as part of the complex network of signaling pathways that crosstalk in a contextual‐dependent manner. These factors, also involved in breast cancer development, are important regulatory nodes for signaling amplification after weaning. Indeed, during involution, p65/p300 target genes such as MMP9, Capn1, and Capn2 are upregulated. Elevated expression and activities of these proteases in breast cancer have been extensively documented. The role of these proteases during mammary gland involution is further discussed. MMPs, calpains, and cathepsins exert their effect by modification of the extracellular matrix and intracellular proteins. Calpains, activated in the mammary gland during involution, cleave several proteins located in cell membrane, lysosomes, mitochondria, and nuclei favoring cell death. Besides, during this period, Capn1 is most probably involved in the modulation of preadipocyte differentiation through chromatin remodeling. Calpains can be implicated in cell anchoring loss, providing a proper microenvironment for tumor growth. A better understanding of the role of any of these proteases in tumorigenesis may yield novel therapeutic targets or prognostic markers for breast cancer. © 2015 IUBMB Life, 67(4):227–238, 2015
Prohibitin 2: At a communications crossroads
Authors: Bavelloni Alberto , Piazzi Manuela , Raffini Mirco , Faenza Irene , Blalock William L. 
Published: April 2015

AbstractProhibitins (PHBs) are a highly conserved class of proteins first discovered as inhibitors of cellular proliferation. Since then PHBs have been found to have a significant role in transcription, nuclear signaling, mitochondrial structural integrity, cell division, and cellular membrane metabolism, placing these proteins among the key regulators of pathologies such as cancer, neuromuscular degeneration, and other metabolic diseases. The human genome encodes two PHB proteins, prohibitin 1 (PHB1) and prohibitin 2 (PHB2), which function not only as a heterodimeric complex, but also independently. While many previous reviews have focused on the better characterized prohibitin, PHB1, this review focuses on PHB2 and new data concerning its cellular functions both in complex with PHB1 and independent of PHB1. © 2015 IUBMB Life, 67(4):239–254, 2015
Preclinical safety evaluation of low molecular weight heparin–deoxycholate conjugates as an oral anticoagulant
Authors: Kim Ji‐young , Jeon Ok‐Cheol , Moon Hyun Tae , Hwang Seung Rim , Byun Youngro 
Published: April 2015

AbstractThe preclinical safety of a newly developed oral anticoagulant, the low molecular weight heparin–deoxycholate conjugate (OH09208), was evaluated by a comprehensive evaluating program in compliance with standard guidelines. The single dose oral toxicity study in rats receiving 2000 and 5000 mg kg−1 of OH09208 did not reveal any mortality, unusual body weight changes or necropsy findings. The results of the 4‐week oral toxicity study with a 4‐week recovery program in rats receiving OH09208 in doses of 100, 300 and 1000 mg kg−1 day−1 did not reveal any mortality, or indicate any unusual clinical signs, or show any toxicokinetic relationships to the administration of OH09208. Although the increase in liver enzymes in one male dog treated with 300 mg kg−1 day−1 and one female dog treated with 1000 mg kg−1 day−1 could not be excluded from the effect of the test substance, no other toxicologically significant changes were observed in the 4‐week oral toxicity study with a 4‐week recovery in beagle dogs. Thus, while the no‐observed‐adverse‐effect level value from the 4‐week study in both male and female rats was 1000 mg kg−1 day−1, those from the 4‐week study in male and female beagle dogs were 300 and 1000 mg kg−1 day−1, respectively. Furthermore, OH09208 did not induce anaphylactic reactions in guinea pigs, micronucleated bone marrow cells in male ICR mice, chromosomal aberration in Chinese hamster lung cell lines, bacterial reverse mutation, and any abnormalities in hERG current assay, mouse central nervous system and dog cardiovascular studies. Overall, there were no unexpected toxicities in this preclinical study that might have precluded the safe administration of OH09208 to humans. Copyright © 2015 John Wiley & Sons, Ltd.
Fibroblastic Synoviocytes Secrete Plasma Proteins Via α2‐Macroglobulins Serving as Intracellular and Extracellular Chaperones
Authors: Zhao Ke‐Wei , Murray Elsa J. Brochmann , Murray Samuel S. 
Published: April 2015

ABSTRACTChanges in plasma protein levels in synovial fluid (SF) have been implicated in osteoarthritis and rheumatoid arthritis. It was previously thought that the presence of plasma proteins in SF reflected ultrafiltration or extravasation from the vasculature, possibly due to retraction of inflamed endothelial cells. Recent proteomic analyses have confirmed the abundant presence of plasma proteins in SF from control and arthritic patients. Systematic depletion of high‐abundance plasma proteins from SF and conditioned media from synoviocytes cultured in serum, and protein analysis under denaturing/reducing conditions have limited our understanding of sources and the native structures of “plasma protein” complexes in SF. Using Western blotting, qPCR, and mass spectrometry, we found that Hig‐82 lapine fibroblastic synovicytes cultured under serum‐free conditions expressed and secreted plasma proteins, including the cytokine‐binding protein secreted phosphoprotein 24 kDa (Spp24) and many of the proteases and protease inhibitors found in SF. Treating synoviocytes with TGF‐β1 or BMP‐2 for 24 h upregulated the expression of plasma proteins, including Spp24, α2‐HS‐glycoprotein, α1‐antitrypsin, IGF‐1, and C‐reactive protein. Furthermore, many of the plasma proteins of mass <151 kDa were secreted as disulfide‐bound complexes with members of the α2‐macroglobulin (A2M) family, which serve as intracellular and extracellular chaperones, not protease inhibitors. Using brefeldin A to block vesicular traffic and protease inhibitors to inhibit endogenous activation of naïve A2M, we demonstrated that the complexes were formed in the endoplasmic reticulum lumen and that Ca2+cysteine protease‐dependent processes are involved. J. Cell. Biochem. 116: 2563–2576, 2015. Published 2015. This article is a U.S. Government work and is in the public domain in the USA
miR‐195a Inhibits Adipocyte Differentiation by Targeting the Preadipogenic Determinator Zfp423
Authors: Yun Ui Jeong , Song No‐Joon , Yang Dong Kwon , Kwon So‐Mi , Kim Kwangho , Kim Sunghwan , Jo Dong‐Gyu , Park Woo Jin , Park Kye Won , Kang Hara 
Published: April 2015

ABSTRACTMicroRNAs (miRNAs) play essential roles in various cellular processes including proliferation and differentiation. In this study, we identified miRNA‐195a (miR‐195a) as a regulator of adipocyte differentiation. Differential expression of miR‐195a in preadipocytes and adipocytes suggests its role in lipid accumulation and adipocyte differentiation. Forced expression of miR‐195a mimics suppressed lipid accumulation and inhibited expression of adipocyte markers such as PPARγ and aP2 in 3T3‐L1 and C3H10T1/2 cells. Conversely, downregulation of miR‐195a by anti‐miR‐195a increased lipid accumulation and expression of adipocyte markers. Target prediction analysis suggested zinc finger protein 423 (Zfp423), a preadipogenic determinator, as a potential gene recognized by miR‐195a. In line with this, mimicked expression of miR‐195a reduced the expression of Zfp423, whereas anti‐miR‐195a increased its expression. Predicted targeting sequences in Zfp423 3'UTR, but not mutated sequences fused to luciferase, were regulated by miR‐195a. Ectopic Zfp423 expression in 3T3‐L1 cells increased lipid accumulation and expression of adipocyte markers, consistent with the observation that miR‐195a targets Zfp423, resulting in suppressed adipocyte differentiation. In addition, miR‐195a and Zfp423 were inversely correlated in obese fat tissues, raising the possibility of miRNA's role in obesity. Together, our data show that miR‐195a is an anti‐adipogenic regulator, which acts by targeting Zfp423, and further suggest the roles of miR‐195a in obesity and metabolic diseases. J. Cell. Biochem. 116: 2589–2597, 2015. © 2015 Wiley Periodicals, Inc.
Febuxostat improves outcome in a rat model of cancer cachexia
Authors: Konishi Masaaki , Pelgrim Loes , Tschirner Anika , Baumgarten Anna , von Haehling Stephan , Palus Sandra , Doehner Wolfram , Anker Stefan D. , Springer Jochen 
Published: April 2015

AbstractBackgroundActivity of xanthine oxidase is induced in cancer cachexia, and its inhibition by allopurinol or oxypurinol improves survival and reduces wasting in the Yoshida hepatoma cancer cachexia model. Here, we tested the effects of the second‐generation xanthine oxidase inhibitor febuxostat compared with placebo in the same model as used previously by our group.MethodsWistar rats (~200 g) were treated daily with febuxostat at 5 mg/kg/day or placebo via gavage for a maximum of 17 days. Weight change, quality of life, and body composition were analysed. After sacrifice, proteasome activity in the gastrocnemius muscle was measured. Muscle‐specific proteins involved in metabolism were analysed by western blotting.ResultsTreatment of the tumour‐bearing rats with febuxostat led to a significantly improved survival compared with placebo (hazard ratio: 0.45, 95% confidence interval: 0.22–0.93, P = 0.03). Loss of body weight was reduced (−26.3 ± 12.4 g) compared with placebo (−50.2 ± 2.1 g, P < 0.01). Wasting of lean mass was attenuated (−12.7 ± 10.8 g) vs. placebo (−31.9 ± 2.1 g, P < 0.05). While we did not see an effect of febuxostat on proteasome activity at the end of the study, the pAkt/Akt ratio was improved by febuxostat (0.94 ± 0.09) vs. placebo (0.41 ± 0.05, P < 0.01), suggesting an increase in protein synthesis.ConclusionsFebuxostat attenuated cachexia progression and improved survival of tumour‐bearing rats.
Effects of temperature and humidity on atomic force microscopy dimensional measurement
Authors: Wang Tian , Ma Chengfu , Chen Yuhang , Chu Jiaru , Huang Wenhao 
Published: April 2015

ABSTRACTThe influence of environmental factors on dimensional measurements of atomic force microscopy (AFM) was investigated experimentally. Measurements were taken with environmental control over a whole AFM chamber and a local sample chamber to highlight the influence of working conditions on the instrument itself. Both temperature and humidity were found to have a significant impact on pitch measurements of a two‐dimensional grating. The effect of temperature on the behavior of the microscope itself is generally larger than the thermal expansion or contraction of the sample. The effect of humidity was further determined to be relevant to the scan direction and velocity. For precise AFM dimensional measurements, the possible influences of temperature and humidity must be carefully considered. Microsc. Res. Tech. 78:562–568, 2015. © 2015 Wiley Periodicals, Inc.
Comparable outcome between endometrioid and non‐endometrioid tumors in patients with early‐stage high‐grade endometrial cancer
Authors: Reynaers E.A.E.M. , Ezendam N.P.M. , Pijnenborg J.M.A. 
Published: April 2015

BackgroundApproximately 25% of endometrial cancer patients present with high‐grade tumors. Unlike the clearly defined work‐up for non‐endometrioid endometrial cancer, no consensus exists for surgical staging and adjuvant therapy in high‐grade endometrioid endometrial cancer. We compared the recurrence rate and disease‐related mortality (DRM) after treatment between endometrioid and non‐endometrioid endometrial cancer.MethodsA total of 123 patients diagnosed with early‐stage high‐grade endometrial cancer at the Dutch Comprehensive Cancer Centre South (CCCS) between January 2005 and December 2011 were included. All patients underwent abdominal hysterectomy and bilateral salpingo‐oophorectomy. Patient and tumor characteristics, primary and adjuvant treatment, and outcome were analyzed.ResultsAfter a median follow‐up of 27.9 months, 27.6% (n = 34) of patients had recurrent disease. Distant recurrence rate was equal among endometrioid (14.5%), papillary serous (14.8%), and clear cell (15.4%) types. The total DRM was 15.4% (n = 19). The 5 year recurrence‐free survival was not significantly different between early‐stage high‐grade endometrioid versus non‐endometrioid endometrial cancer (P = 0.72).ConclusionDistant recurrence and DRM was high in patients with endometrial cancer regardless of histological type, suggesting the need for different therapies in early‐stage high‐grade non‐endometrioid and endometrioid tumors. J. Surg. Oncol. 2015 111:790–794. © 2015 Wiley Periodicals, Inc.
The influence of added carbon nanotubes on the properties of the carbon‐fiber‐reinforced paper‐based wet clutch friction materials
Authors: Wang Wen‐jing , Huang Jian‐feng , Li Wen‐bin , Jin Yu‐guang , Wei Fei 
Published: April 2015

AbstractDifferent carbon nanotubes (CNT) content was used to investigate the influence on physical properties, microstructure, tribological behaviour and thermal endurance properties of the materials. Experimental results show that the bulk density of the specimens increase with the increase of the CNT content, and the open porosity of the samples gradually reduced, whereas the CNT content increases. When comparing the tribological properties of the specimens with different CNT content, specimens that contain 4 wt.% carbon nanotubes show the highest dynamic friction coefficient and the lowest variation coefficient of the dynamic friction coefficient. Moreover, the specimens exhibit more thermal stability and lower weight loss after adding the CNT. Copyright © 2015 John Wiley & Sons, Ltd.
Change Their Choice! Changing Behavior Using the CAN Approach and Activism Research
Authors: Wansink Brian 
Published: April 2015

ABSTRACTMost of our research on eating behavior has no impact on health or public policy. Part is due to the nontranslational way we often conduct our studies; part is due to us not having a useful framework that organizes our conclusions. This paper's first purpose is to offer an organizing framework that shows how nearly all effective interventions on food choice either make healthy choices more convenient (physically or cognitively), more attractive (comparatively or absolutely), or more normal (perceived or actual). This paper's second purpose is to introduce the notion of activism research—an approach to designing and executing studies in a way that makes consumer psychology research more actionable, useful, effective, and scalable. Together these two tools could help expand both the relevance and reach, and impact of what we do.