bluuebunny/crossref_metadata_2025_split huggingface dataset:
The combined expression of the stromal markers fibronectin and SPARC improves the prediction of survival in diffuse large B-cell lymphoma
Authors: Brandt Simone , Montagna Chiara , Georgis Antoin , Schüffler Peter J , Bühler Marco M , Seifert Burkhardt , Thiesler Thore , Curioni-Fontecedro Alessandra , Hegyi Ivan , Dehler Silvia , Martin Vittoria , Tinguely Marianne , Soldini Davide 
Published: October 2013

Abstract

Background
In diffuse large B-cell lymphomas, gene expression profiling studies attributed a major biologic role to non-neoplastic cells of the tumour microenvironment as its composition and characteristics were shown to predict survival. In particular, the expression of selected genes encoding components of the extracellular matrix was reported to be associated with clinical outcome. Nevertheless, the translation of these data into robust, routinely applicable immunohistochemical markers is still warranted. Therefore, in this study, we analysed the combination of the expression of the extracellular matrix components Fibronectin and SPARC on formalin-fixed paraffin embedded tissue derived from 173 patients with DLBCL in order to recapitulate gene expression profiling data.

Results
The expression of Fibronectin and SPARC was detected in 77/173 (44.5%) and 125/173 (72.3%) cases, respectively, and 55/173 (31.8%) cases were double positive. Patients with lymphomas expressing Fibronectin showed significantly longer overall survival when compared to negative ones (6.3 versus 3.6 years). Moreover, patients with double positive lymphomas also presented with significantly longer overall survival when compared with the remaining cases (11.6 versus 3.6 years) and this combined expression of both markers results in a better association with overall survival data than the expression of SPARC or Fibronectin taken separately (Hazard ratio 0.41, 95% confidence interval 0.17 to 0.95, p = 0.037). Finally, neither Fibronectin nor SPARC expression was associated with any of the collected clinico-pathological parameters.

Conclusions
The combined immunohistochemical assessment of Fibronectin and SPARC, two components of the extracellular matrix, represents an important tool for the prediction of survival in diffuse large B-cell lymphomas. Our study suggests that translation of gene expression profiling data on tumour microenvironment into routinely applicable immunohistochemical markers is a useful approach for a further characterization of this heterogeneous type of lymphoma.
The presence and prognostic impact of apoptotic and nonapoptotic disseminated tumor cells in the bone marrow of primary breast cancer patients after neoadjuvant chemotherapy
Authors: Hartkopf Andreas Daniel , Taran Florin-Andrei , Wallwiener Markus , Hagenbeck Carsten , Melcher Carola , Krawczyk Natalia , Hahn Markus , Wallwiener Diethelm , Fehm Tanja 
Published: October 2013

Abstract
Introduction
Neoadjuvant systemic therapy of primary breast cancer (PBC) patients offers the possibility to monitor treatment response. However, patients might have metastatic relapse despite achieving a pathologic complete response (pCR). This indicates that local response to therapy must not be representative for systemic treatment efficacy. Therefore, the aim of this study was to compare local response with systemic tumor cell dissemination by determining the presence of disseminated tumor cells (DTCs), including apoptotic tumor cells, in the bone marrow (BM) of PBC patients after neoadjuvant chemotherapy (NACT).

Methods
DTCs were detected by immunocytochemistry (pancytokeratin antibody A45-B/B3) and cytomorphology (DTC status). The presence of apoptotic tumor cells was determined by using the M30 antibody (M30 status). This antibody detects a neo-epitope that is expressed only during early apoptosis.

Results
BM aspirates from 400 PBC patients that had completed NACT were eligible for this study. Of these, 167 (42%) patients were DTC positive (DTC status). The M30 status was investigated in 308 patients. Apoptotic (M30-positive) tumor cells were detected in 89 (29%) of these. Whereas the DTC status was not correlated (P = 0.557) to local treatment response (that is, pCR or a clinical complete/partial response), the presence of M30-positive tumor cells was significantly higher in patients that responded to therapy (P = 0.026). Additionally, DTC-positive patients were at an increased risk for disease relapse (hazard ratio, 1.87; 95% CI, 1.11 to 3.15; P = 0.019).

Conclusion
The presence of DTC is independent of therapy response of the primary tumor. As patients that are DTC positive after NACT have an unfavorable outcome, they might benefit from additional systemic treatment.
Net Clinical Benefit Analysis of Radiation Therapy Oncology Group 0525: A Phase III Trial Comparing Conventional Adjuvant Temozolomide With Dose-Intensive Temozolomide in Patients With Newly Diagnosed Glioblastoma
Authors: Armstrong Terri S. , Wefel Jeffrey S. , Wang Meihua , Gilbert Mark R. , Won Minhee , Bottomley Andrew , Mendoza Tito R. , Coens Corneel , Werner-Wasik Maria , Brachman David G. , Choucair Ali K. , Mehta Minesh 
Published: October 2013

Purpose Radiation Therapy Oncology Group trial 0525 tested whether dose-intensifying temozolomide versus standard chemoradiotherapy improves overall survival (OS) or progression-free survival (PFS) in newly diagnosed glioblastoma. Tests of neurocognitive function (NCF) and symptoms (using the MD Anderson Symptom Inventory–Brain Tumor module; MDASI-BT) and of quality of life (European Organisation for the Research and Treatment of Cancer Quality of Life Questionnaire [EORTC QLQ] –C30/BN20) examined the net clinical benefit (NCB) of therapy. Patients and Methods NCF tests (Hopkins Verbal Learning Test–Revised, Trail Making Test, and Controlled Oral Word Association), MDASI-BT, and EORTC QLQ-C30/BN20 were completed in a subset of patients. Multivariate Cox proportional hazard regression modeling determined the prognostic value of baseline and early change from baseline to cycle 1 for OS and PFS. Two-sample proportional test statistic was used to evaluate differences between treatments (dose-dense v standard-dose) on NCB measures from baseline to cycle 4 in stable patients. Results Overall, 182 patients participated in the study. Baseline NCF tests and the physical functioning quality of life scale were associated with OS and PFS. Baseline to cycle 1 in all NCB components were associated with OS and PFS. There was greater deterioration in the dose-dense arm from baseline to cycle 4 in the Global Health and Motor Function subscales (EORTC QLQ-C30/BN20) as well as in overall symptom burden, overall symptom interference, and activity-related symptom interference subscales (MDASI-BT). There were no between-arm differences in NCF. Conclusion Longitudinal collection of NCB measures is feasible in cooperative group studies and provides an added dimension to standard outcome measures. Greater adverse symptom burden and functional interference, as well as decreased global health and motor function were observed in patients randomly assigned to the dose-dense arm. Baseline and early change in NCB measures were associated with decreased rates of survival.
Muslim Algerian Women and the Rights of Man: Islam and Gendered Citizenship in French Algeria at the End of Empire
Authors: Wadowiec Jaime 
Published: October 2013

Against recent scholarship that has examined Algerian women’s roles as partisans in the colonial war (1954-62), this article highlights a small but diverse segment of women who entered into the politics of decolonization as French citizens rather than as anticolonial nationalists. Given equal citizenship in 1958, these women took seriously French efforts aimed at the political integration of the Muslim Algerian population as a peaceful solution to the Algerian War. As self-professed françaises musulmanes, or as “French Muslim” citizens, these women advanced a platform highlighting Muslim women’s social rights rather than national independence suggesting, often directly, that the French state could better guarantee their equality than male nationalists. The article situates their civic demands as an expression of a nascent grassroots feminism and proceeds with an examination of women’s critique of the secular, consumer-oriented model of gendered citizenship offered to them by the French Fifth Republic.
Contrairement à de récentes études qui ont examiné le rôle de partisanes algériennes pendant la guerre coloniale (1954-62), cet article s’intéresse à un groupe de femmes, petit mais diversifié, qui sont entrées dans la politique de décolonisation en tant que citoyennes françaises plutôt que nationalistes anticolonialistes. La pleine citoyenneté leur avait été accordée en 1958 et ces femmes prenaient au sérieux les efforts français visant l’intégration politique de la population algérienne musulmane pour trouver une issue pacifique à la guerre d’Algérie. En tant que Françaises musulmanes auto-proclamées, ou en tant que citoyennes « françaises musulmanes », ces femmes présentèrent une plate-forme qui soulignait les droits sociaux des femmes musulmanes plutôt que l’indépendance nationale suggérant, souvent directement, que l’Etat français était le mieux à même de garantir leur égalité que les nationalistes hommes. L’article situe leurs exigences civiques comme une expression du féminisme « populaire » naissant avant d’examiner leur point de vue sur le modèle de citoyenneté sexuée laïque et axé sur la consommation qui leur était offert par la Cinquième République française.
Expandability of Ultralong C–C Bonds: Largely Different C1–C2 Bond Lengths Determined by Low-temperature X-ray Structural Analyses on Pseudopolymorphs of 1,1-Bis(4-fluorophenyl)-2,2-bis(4-methoxyphenyl)pyracene
Authors: Suzuki Takanori , Uchimura Yasuto , Nagasawa Fumika , Takeda Takashi , Kawai Hidetoshi , Katoono Ryo , Fujiwara Kenshu , Murakoshi Kei , Fukushima Takanori , Nagaki Aiichiro , Yoshida Jun-ichi 
Published: October 2013

Abstract
Low-temperature X-ray analyses on several pseudopolymorphs (solvate crystals) revealed that the C1–C2 bond length of the highly congested title molecule can adopt quite different values [1.700(6)–1.739(6) Å]. Such an unusual observation indicates that the ultralong covalent bond is endowed with “expandability,” thus the prestrained bond can be elongated or shortened very easily accompanied by only a minute change in energy, which can be compensated by intermolecular perturbation in the crystal.
Innovation or disruption? The National Broadband Network comes to Australian TV
Authors: Morsillo Robert , Barr Trevor 
Published: October 2013

Abstract
There are many forces for change confronting the well-established institutional arrangements underpinning Australian media industries, with commercial television, in particular, likely to be most challenged during the next five years. New distribution and delivery models connected to the proposed high capacity National Broadband Network (NBN), along with new content providers and changing viewer preferences are likely to drive major changes to existing television arrangements. In a rapidly changing environment, this article seeks to relate established concepts of innovation and creative destruction, disintermediation and disruption to the impact these new NBN mediated opportunities may have on existing TV arrangements, both free-to-air (FTA) and subscription (STV). It seeks to explore the extent to which TV-like services over the NBN might disrupt incumbent TV broadcasters; the extent to which changing consumer preferences and practices might disrupt current business models; and how incumbent TV broadcasters might be responding to these threats with their own innovations.
Policies for the development of digital terrestrial television in Argentina
Authors: Mastrini Guillermo , Becerra Martín , Bizberge Ana , Krakowiak Fernando 
Published: October 2013

Abstract
Following the ups-and-downs of the selection of the digital TV standard, in the second half of 2009 Argentina decided to take the same path as Brazil, and adopted the Japanese-Brazilian standard. Since then concrete policies and actions have been introduced to implement Digital Terrestrial Television (DTT). Three years into the process, it is possible to put this into perspective and analyse the virtues and deficiencies of DTT deployment in Argentina. This article is intended to focus on the public policies introduced by the Argentine State both in the deployment of the necessary infrastructure and in the stimulus to content production. What is special about the Argentine case is the leading role of the State.
The content management of media in China
Authors: Zhao Yu 
Published: October 2013

Abstract
China is famous for its stringent regulations and everyday monitoring of media content. Empowered by law and regulation, the state authorities govern the content industry through ownership control and the licensing system. So far individuals and entities outside the state’s control are not entitled to access the content production market, and the externally originated content is under strict supervision. The bottom line of media content management is to ensure that the publicized political content advocates the party-government line, which does not necessarily mean that the media cannot criticize the government but that they can never mobilize or organize collective actions without permission. The effectiveness over media content control has crumbled dramatically, and for China in today’s world it is no longer simple to manage a rapidly changing media environment.
Estrategias de comunicación 2.0 en asociaciones profesionales. Estudio del caso de los Colegios Oficiales de Médicos en España
Authors: Sánchez-González María , Paniagua-Rojano Francisco-Javier 
Published: October 2013

El presente trabajo se centra en la estrategia de comunicación en red de los Colegios Oficiales de Médicos españoles, especialmente en lo referido al uso de redes sociales y otras herramientas de la denominada web social y a la integración de éstas y aquellas tradicionales en sus webs institucionales. Con este objetivo, primero se han identificado los públicos internos y externos de estos organismos y se ha descrito, a un nivel teórico, la redefinición de los roles y funciones de los gabinetes de comunicación en el contexto actual. A partir de ahí se ha desarrollado un análisis que ha permitido determinar, en cada uno de los casos, la tipología de sala de prensa online en función de las herramientas empleadas, la presencia de la asociación en los principales sitios de redes sociales y en otros espacios de la web social para la publicación de contenido multimedia, y la visibilidad y conexión con estos canales externos desde dichas salas de prensa online.
Educating Nurses for the Twenty-First Century Abilities-Based Outcomes and Assessing Student Learning in the Context of Democratic Professionalism
Authors: Thompson Janice L. , Mallet-Boucher Monique , McCloskey Claudia , Tamlyn Karen , Wilson Kathy 
Published: October 2013

AbstractThis article describes recent work in implementing a framework of abilities-based learning outcomes and related practices for assessing student learning in a Canadian Baccalaureate nursing degree program. The authors describe steps in implementing an abilities-based paradigm for nursing education, and subsequently developing an assessment framework based on abilities-based learning outcomes. The discussion reviews theoretical and policy-level influences involved in this initiative. The curriculum work is also discussed in terms of current trends in higher education emphasizing the assessment of student learning. The discussion concludes by linking these efforts to recent calls for a paradigm shift in the preparation of professionals, noting how the assessment of student learning can contribute to democratic professional formation in nursing.
A Deep Intronic Mutation in the Ankyrin-1 Gene Causes Diminished Protein Expression Resulting in Hemolytic Anemia in Mice
Authors: Huang Hua , Zhao PengXiang , Arimatsu Kei , Tabeta Koichi , Yamazaki Kazuhisa , Krieg Lara , Fu Emily , Zhang Tian , Du Xin 
Published: October 2013

Abstract
Linkage between transmembrane proteins and the spectrin-based cytoskeleton is necessary for membrane elasticity of red blood cells. Mutations of the proteins that mediate this linkage result in various types of hemolytic anemia. Here we report a novel N-ethyl-N-nitrosourea−induced mutation of ankyrin-1, named hema6, which causes hereditary spherocytosis in mice through a mild reduction of protein expression. The causal mutation was traced to a single nucleotide transition located deep into intron 13 of gene Ank1. In vitro minigene splicing assay revealed two abnormally spliced transcripts containing cryptic exons from fragments of Ank1 intron 13. The inclusion of cryptic exons introduced a premature termination codon, which leads to nonsense-mediated decay of the mutant transcripts in vivo. Hence, in homozygous mice, only wild-type ankyrin-1 is expressed, albeit at 70% of the level in wild-type mice. Heterozygotes display a similar hereditary spherocytosis phenotype stemming from intermediate protein expression level, indicating the haploinsufficiency of the mutation. Weakened linkage between integral transmembrane protein, band 3, and underlying cytoskeleton was observed in mutant mice as the result of reduced high-affinity binding sites provided by ankyrin-1. Hema6 is the only known mouse mutant of Ank1 allelic series that expresses full-length canonical ankyrin-1 at a reduced level, a fact that makes it particularly useful to study the functional impact of ankyrin-1 quantitative deficiency.
Cytoplasmic Male Sterility Contributes to Hybrid Incompatibility Between Subspecies ofArabidopsis lyrata
Authors: Aalto Esa A , Koelewijn Hans-Peter , Savolainen Outi 
Published: October 2013

AbstractIn crosses between evolutionarily diverged populations, genomic incompatibilities may result in sterile hybrids, indicating evolution of reproductive isolation. In several plant families, crosses within a population can also lead to male sterile progeny because of conflict between the maternally and biparentally inherited genomes. We examined hybrid fertility between subspecies of the perennial outcrossing self-incompatible Lyrate rockcress (Arabidopsis lyrata) in large reciprocal F2 progenies and three generations of backcrosses. In one of the reciprocal F2 progenies, almost one-fourth of the plants were male-sterile. Correspondingly, almost one-half of the plants in one of the four reciprocal backcross progenies expressed male sterility. In an additional four independent F2 and backcross families, three segregated male sterility. The observed asymmetrical hybrid incompatibility is attributable to male sterility factors in one cytoplasm, for which the other population lacks effective fertility restorers. Genotyping of 96 molecular markers and quantitative trait locus mapping revealed that only 60% of the plants having the male sterile cytoplasm and lacking the corresponding restorers were phenotypically male-sterile. Genotyping data showed that there is only one restorer locus, which mapped to a 600-kb interval at the top of chromosome 2 in a region containing a cluster of pentatricopeptide repeat genes. Male fertility showed no trade-off with seed production. We discuss the role of cytoplasm and genomic conflict in incipient speciation and conclude that cytoplasmic male sterility–lowering hybrid fitness is a transient effect with limited potential to form permanent reproductive barriers between diverged populations of hermaphrodite self-incompatible species.
Central Line Maintenance Bundles and CLABSIs in Ambulatory Oncology Patients
Authors: Rinke Michael L. , Bundy David G. , Chen Allen R. , Milstone Aaron M. , Colantuoni Elizabeth , Pehar Miriana , Herpst Cynthia , Fratino Lisa , Miller Marlene R. 
Published: October 2013

OBJECTIVE:
Pediatric oncology patients are frequently managed with central lines as outpatients, and these lines confer significant morbidity in this immune-compromised population. We aimed to investigate whether a multidisciplinary, central line maintenance care bundle reduces central line–associated bloodstream infections (CLABSIs) and bacteremias in ambulatory pediatric oncology patients.

METHODS:
We conducted an interrupted time-series study of a maintenance bundle concerning all areas of central line care. Each of 3 target groups (clinic staff, homecare agency nurses, and patient families) (1) received training on the bundle and its importance, (2) had their practice audited, and (3) were shown CLABSI rates through graphs, in-service training, and bulletin boards. CLABSI and bacteremia person-time incidence rates were collected for 23 months before and 24 months after beginning the intervention and were compared by using a Poisson regression model.

RESULTS:
The mean CLABSI rate decreased by 48% from 0.63 CLABSIs per 1000 central line days at baseline to 0.32 CLABSIs per 1000 central line days during the intervention period (P = .005). The mean bacteremia rate decreased by 54% from 1.27 bacteremias per 1000 central line days at baseline to 0.59 bacteremias per 1000 central line days during the intervention period (P &lt; .001).

CONCLUSIONS:
Implementation of a multidisciplinary, central line maintenance care bundle significantly reduced CLABSI and bacteremia person-time incidence rates in ambulatory pediatric oncology patients with central lines. Further research is needed to determine if maintenance care bundles reduce ambulatory CLABSIs and bacteremia in other adult and pediatric populations.
Approval and Perceived Impact of Duty Hour Regulations: Survey of Pediatric Program Directors
Authors: Drolet Brian C. , Whittle Sarah B. , Khokhar Mamoona T. , Fischer Staci A. , Pallant Adam 
Published: October 2013

OBJECTIVES:
To determine pediatric program director (PD) approval and perception of changes to resident training and patient care resulting from 2011 Accreditation Council for Graduate Medical Education (ACGME) Common Program Requirements.

METHODS:
All US pediatric PDs (n = 181) were identified from the ACGME. Functional e-mail addresses were identified for 164 (90.6%). Three individualized e-mail requests were sent to each PD to complete an anonymous 32-question Web-based survey.

RESULTS:
A total of 151 responses were obtained (83.4%). Pediatrics PDs reported approval for nearly all of the 2011 ACGME duty hour regulations except for 16-hour intern shift limits (72.2% disapprove). Regarding the perceived impact of the new standards, many areas were reportedly unchanged, but most PDs reported negative effects on resident education (74.7%), preparation for senior roles (79.9%), resident ownership of patients (76.8%), and continuity of care (78.8%). There was a reported increase in PD workload (67.6%) and use of physician extenders (62.7%). Finally, only 48.3% of PDs reported that their residents are “always” compliant with 2011 requirements.

CONCLUSIONS:
Pediatric PDs think there have been numerous negative consequences of the 2011 Common Program Requirements. These include declines in resident education and preparation to take on more senior roles, as well as diminished resident accountability and continuity of care. Although they support individual aspects of duty hour regulation, almost three-quarters of pediatric PDs say there should be fewer regulations. The opinions expressed by PDs in this study should prompt research using quantitative metrics to assess the true impact of duty hour regulations.