bluuebunny/crossref_metadata_2025_split huggingface dataset:
Low‐level laser therapy promotes the osteogenic potential of adipose‐derived mesenchymal stem cells seeded on an acellular dermal matrix
Authors: Choi Kyuseok , Kang Byung‐Jae , Kim Hyoju , Lee Seungmin , Bae Sohee , Kweon Oh‐Kyeong , Kim Wan Hee 
Published: March 2013

AbstractThis study investigates the feasibility of using an adipose‐derived mesenchymal stem cell (ASC)‐seeded acellular dermal matrix (ADM) along with low‐level laser therapy (LLLT) to repair bone defect in athymic nude mice. Critical‐sized calvarial defects were treated either with ADM, ADM/LLLT, ADM/ASCs, or ADM/ASCs/LLLT. In micro‐computed tomography scans, the ADM/ASCs and the ADM/ASCs/LLLT groups showed remarkable bone formation after 14 days. Additionally, bone regeneration in the ADM/ASCs/LLLT group was obvious at 28 days, but in the ADM/ASCs group at 56 days. Bone mineral density and bone tissue volume in the ADM/ASCs/LLLT group significantly increased after 7 days, but in the ADM/ASCs group after 14 days. Histological analysis revealed that the defects were repaired in the ADM/ASCs and the ADM/ASCs/LLLT group, while the defects in the ADM and the ADM/LLLT groups exhibited few bone islands at 28 and 56 days. The successful seeding of ASCs onto ADM was confirmed, and LLLT enhanced the proliferation and the survival of ASCs at 14 days. Our results indicate that ASC‐seeded grafts promote bone regeneration, and the application of LLLT on ASC‐seeded ADM results in rapid bone formation. The implantation of an ASC‐seeded ADM combined with LLLT may be used effectively for bone regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Local drug delivery by personalized, intraoperative custom‐made implant coating
Authors: Trajkovski B. , Petersen A. , Perka C. , Scharnagl N. , Wischke C. , Wagermaier W. , Lendlein A. , Duda G. N. 
Published: March 2013

AbstractLocal administration of drugs can enhance regeneration, prevent infection, or treat postsurgical pain. If used in conjunction with implants, coating strategies should allow the choice of a drug or combination of drugs, their doses, localization, and release due to intraoperative considerations. Current coating technologies lack the ability for personalized medicine strategies. Here, we describe a new intraoperative strategy for drug delivery that allows a personalized approach as local drug delivery by implant coating. A polyvinylalcohol (PVA) patch provides rapid attachment to implant surfaces by cyanoacrylate (CA) adhesives. The CA polymerization was initiated by water uptake of the patch due to exposure to a humid environment. The coating strength depended on the type of the CA, the time of external pressing load and humidification, the properties of the patch and the implant surface. The CA adhesive penetrated and polymerized within the patch without impeding the bioactivity of the embedded molecules or strongly altering the protein release pattern after attachment to the implant surface. The use of CA in combination with the PVA patch proved to be noncytotoxic in vitro. This technology platform opens the possibility for personalized medicine to locally administer drugs due to intraoperative requirements. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Fibronectin fixation on poly(ethyl acrylate)–based copolymers
Authors: Briz N. , Antolinos‐Turpin C. M. , Alió J. , Garagorri N. , Ribelles J. L. Gómez , Gómez‐Tejedor J. A. 
Published: March 2013

AbstractThe aim of this paper is to quantify the adhered fibronectin (FN; by adsorption and/or grafting) and the exposure of its cell adhesive motifs (RGD and FNIII7‐10) on poly(ethyl acrylate) (PEA) copolymers whose chemical composition has been designed to increase wettability and to introduce acid functional groups. FN was adsorbed to PEA, poly(ethyl acrylate‐co‐hydroxyethyl acrylate), poly(ethyl acrylate‐co‐acrylic acid), and poly(ethyl acrylate‐co‐methacrylic acid) copolymers, and covalently cross‐linked to poly(ethyl acrylate‐co‐acrylic acid) and poly(ethyl acrylate‐co‐methacrylic acid) copolymers. Amount of adhered FN and exhibition of RGD and FNIII7–10 fragments involved in cell adhesion were quantified with enzyme‐linked immunosorbent assay tests. Even copolymers with a lower content of the hydrophilic component showed a decrease in water contact angle. In addition, FN was successfully fixed on all surfaces, especially on the hydrophobic surfaces. However, it was demonstrated that exposure of its cell adhesion sequences, which is the key factor in cell adhesion and proliferation, was higher for hydrophilic surfaces. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Influence of water and sediment supply on the stratigraphic record of alluvial fans and deltas: Process controls on stratigraphic completeness
Authors: Straub Kyle M. , Esposito Christopher R. 
Published: March 2013

AbstractStratigraphy contains the most complete record of information necessary to quantitatively reconstruct paleolandscape dynamics, but this record contains significant gaps over a range of time and space scales. These gaps result from stasis on geomorphic surfaces and erosional events that remove previously deposited sediment. Building on earlier statistical studies, we examine stratigraphic completeness in three laboratory experiments where the topography of aggrading deltas was monitored at high temporal and spatial scales. The three experiments cover unique combinations in the absolute magnitudes of sediment and water discharge in addition to generation of accommodation space through base‐level rise. This analysis centers on three time scales: (1) the time at which a record is discretized (t), (2) the time necessary to build a deposit with mean thickness equivalent to the maximum roughness on a surface (Tc), and (3) the time necessary for channelized flow to migrate over all locations in a basin (Tch). These time scales incorporate information pertaining to the time‐variant topography of actively changing surfaces, kinematics by which the surfaces are changing, and net deposition rate. We find that stratigraphic completeness increases as a function of t/Tc but decreases as a function of Tc/Tch over the parameter space covered in the experiments. Our results suggest that environmental signals disconnected from a sediment routing system are best preserved in systems with low Tc values. Nondimensionalizing t by Tc, however, shows that preservation of information characterizing system morphodynamics is best preserved in stratigraphy constructed by systems with low water to sediment flux ratios.
Prevalence and genetic diversity of norovirus infection in Tunisian children (2007–2010)
Authors: Hassine‐Zaafrane Mouna , Sdiri‐Loulizi Khira , Kaplon Jérôme , Salem Imen Ben , Pothier Pierre , Aouni Mahjoub , Ambert‐Balay Katia 
Published: March 2013

AbstractViral gastroenteritis can be a life‐threatening disease in infants and young children, especially in developing countries. The aim of this study was to continue the epidemiological surveillance of norovirus (NoV) infections in Tunisian children suffering from acute gastroenteritis. Surveillance was initiated in January 2003, to monitor potential variations in strains over time, in terms of frequency and diversity of NoV genotypes, and more particularly the potential emergence of new GII.4 variants following the 2004 Hunter variant. From April 2007 to April 2010, a total of 407 stool specimens were collected from sporadic cases (238 inpatients and 169 outpatients). Furthermore, 28 stool samples were collected from children involved in 3 gastroenteritis outbreaks. Stool specimens were screened for NoV genogroup I (GI) and II (GII) by RT‐PCR. NoV strains were genotyped, and variants identified, based on sequence and phylogenetic analyses of the polymerase and capsid genes. NoVs were detected in 38 sporadic cases (9.3%) and 21 epidemic cases (75%). Great diversity was observed throughout the period, with seven distinct NoV genotypes characterized in sporadic cases, and three in outbreaks. GIIb/II.3 and GII.4 were predominant globally, with fluctuations of their prevalence over time. Interestingly, the Hunter variant, which was the unique GII.4 variant observed from 2003 to April 2007 in the region of Monastir, was replaced by the 2006b variant. NoV is an important enteropathogen responsible for viral gastroenteritis among infants and children in Tunisia, and the infecting strains between 2007 and 2010 were different from those in previous years. J. Med. Virol. 85: 1100–1110, 2013. © 2013 Wiley Periodicals, Inc.
Protoporphyrin IX Fluorescence and Photobleaching During Interstitial Photodynamic Therapy of Malignant Gliomas for Early Treatment Prognosis
Authors: Johansson Ann , Faber Florian , Kniebühler Gesa , Stepp Herbert , Sroka Ronald , Egensperger Rupert , Beyer Wolfgang , Kreth Friedrich‐Wilhelm 
Published: March 2013

AbstractBackground and ObjectiveInterstitial photodynamic therapy (iPDT) of non‐resectable recurrent glioblastoma using 5‐aminolevulinic acid (ALA)‐induced protoporphyrin IX (PpIX) has shown a promising outcome. It remained unclear, however, to what extent inter‐ and intra‐tumoural differences of PpIX concentrations influence the efficacy of iPDT. In the current pilot study, we analysed PpIX concentrations quantitatively and assessed PpIX induced fluorescence and photobleaching intraoperatively.Materials and MethodsFive patients harbouring non‐resectable glioblastomas were included. ALA (20 or 30 mg/kg body weight) was given 5–8 hours before treatment. Stereotactic biopsies were taken throughout the tumour volume for both histological analysis and determination of PpIX concentrations, which were measured by chemical extraction. Cylindrical light diffusors were stereotactically implanted. Prior to and after irradiation, fluorescence measurements were performed. Outcome measurement was based on clinical and neuro‐radiological follow up.ResultsIn three patients, a strong PpIX fluorescence was seen before treatment, which was completely photobleached after iPDT. High concentrations of PpIX could be detected in viable tumour parts of these patients (mean PpIX uptake per tumour: 1.4–3.0 µM). In the other two patients, however, no or only low PpIX uptake (0–0.6 µM) could be detected. The patients with strong PpIX uptake showed treatment response and long‐term clinical stabilisation (no progression in 29, 30 and 36 months), early treatment failure was seen in the remaining two patients (death after 3 and 9 months).ConclusionsIntra‐tumoural PpIX concentrations exhibited pronounced inter‐ and intra‐tumoural variations in glioblastoma, which are directly linked to variable degrees of fluorescence intensity. High intra‐tumoural PpIX concentrations with strong fluorescence intensity and complete photobleaching after iPDT seem to be associated with favourable outcome. Real‐time monitoring of PpIX fluorescence intensity and photobleaching turned out to be feasible and safe and might be employed for early treatment prognosis of iPDT. Lasers Surg. Med. 9999:XX–XX, 2013. © 2013 Wiley Periodicals, Inc.
A Sensitive and Selective Mercury(II) Sensor Based on Amplified Fluorescence Quenching in a Conjugated Polyelectrolyte/Spiro‐Cyclic Rhodamine System
Authors: Lee Seoung Ho , Parthasarathy Anand , Schanze Kirk S. 
Published: March 2013

AbstractA novel fluorescence‐based sensor for mercury(II) ion is described consisting of a water‐soluble, anionic‐conjugated polyelectrolyte (CPE) and spiro‐cyclic rhodamine derivative (1). A solution containing the CPE (0.1 × 10−6 M repeat unit concentration) and 1 (1 × 10−6 M concentration) in aqueous solution exhibits a sensitive and selective turn‐off fluorescence response to Hg(II). The Stern–Volmer quenching constant for quenching of the fluorescence of the system by Hg(II) is ≈1.5 × 107 M−1, and Hg(II) can be sensed with a detection limit <10 × 10−9 M. A mechanism for the sensor response is proposed and it consists of efficient and selective complex formation between 1 and Hg(II), along with a very high association between the 1‐Hg(II) complex and the anionic CPE.
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Urethral pressure reflectometry during intra‐abdominal pressure increase—an improved technique to characterize the urethral closure function in continent and stress urinary incontinent women
Authors: Saaby Marie‐Louise , Klarskov Niels , Lose Gunnar 
Published: March 2013

AbstractAimto assess the urethral closure function by urethral pressure reflectometry (UPR) during intra‐abdominal pressure‐increase in SUI and continent women.MethodsTwenty‐five urodynamically proven SUI women and eight continent volunteer women were assessed by ICIQ‐SF, pad‐weighing test, incontinence diary, and UPR. UPR was conducted during resting and increased intra‐abdominal pressure (PAbd) by straining. Related values of PAbd and urethral opening pressure (Po) were plotted into an abdomino‐urethral pressuregram. Linear regression of the values was conducted, and the slope of the line (“APIR”) and the intercept with the y‐axis found. By the equation of the line, Po was calculated for various values of PAbd, for example, 50 cm H2O (Po‐Abd 50).ResultsThe resting Po (Po‐rest) and APIR, respectively, significantly differed in SUI and continent women but could not separate the two groups. The urethral closure equation (UCE) based on Po‐rest and APIR provided a more detailed characterization of a woman's closure function based on the permanent closure forces (primarily generated by the urethral sphincteric unit) and the adjunctive closure forces (primarily generated by the support system). Po‐Abd 50 and UCE, respectively, which express the combined permanent and adjunctive closure forces and estimate the efficiency of the closure function, separated SUI and continent women and were highly significantly negatively correlated with ICIQ‐SF, pad test, and the number of incontinence episodes.ConclusionsNew parameters for characterization of the urethral closure function and possible dysfunctions and its efficiency were provided. Po‐Abd 50 and UCE may be used as diagnostic tests and severity measures. Neurourol. Urodynam. 32:1103–1108, 2013. © 2013 Wiley Periodicals, Inc.
An edge‐based smoothed finite element method for 3D analysis of solid mechanics problems
Authors: Cazes F. , Meschke G. 
Published: March 2013

SUMMARYThe edge‐based smoothed finite element method (ES‐FEM) was proposed recently in Liu, Nguyen‐Thoi, and Lam to improve the accuracy of the FEM for 2D problems. This method belongs to the wider family of the smoothed FEM for which smoothing cells are defined to perform the numerical integration over the domain. Later, the face‐based smoothed FEM (FS‐FEM) was proposed to generalize the ES‐FEM to 3D problems. According to this method, the smoothing cells are centered along the faces of the tetrahedrons of the mesh. In the present paper, an alternative method for the extension of the ES‐FEM to 3D is investigated. This method is based on an underlying mesh composed of tetrahedrons, and the approximation of the field variables is associated with the tetrahedral elements; however, in contrast to the FS‐FEM, the smoothing cells of the proposed ES‐FEM are centered along the edges of the tetrahedrons of the mesh. From selected numerical benchmark problems, it is observed that the ES‐FEM is characterized by a higher accuracy and improved computational efficiency as compared with linear tetrahedral elements and to the FS‐FEM for a given number of degrees of freedom. Copyright © 2013 John Wiley & Sons, Ltd.
The influence of environmental hazard maps on risk beliefs, emotion, and health‐related behavioral intentions
Authors: Severtson Dolores J. 
Published: March 2013

AbstractTo test a theoretical explanation of how attributes of mapped environmental health hazards influence health‐related behavioral intentions and how beliefs and emotion mediate the influences of attributes, 24 maps were developed that varied by four attributes of a residential drinking water hazard: level, proximity, prevalence, and density. In a factorial design, student participants (N = 446) answered questions about a subset of maps. Hazard level and proximity had the largest influences on intentions to test water and mitigate exposure. Belief in the problem's seriousness mediated attributes' influence on intention to test drinking water, and perceived susceptibility mediated the influence of attributes on intention to mitigate risk. Maps with carefully illustrated attributes of hazards may promote appropriate health‐related risk beliefs, intentions, and behavior. © 2013 Wiley Periodicals, Inc. Res Nurs Health 36: 330–348, 2013
Clinical practice guidelines for the perioperative nutritional, metabolic, and nonsurgical support of the bariatric surgery patient—2013 update: Cosponsored by american association of clinical endocrinologists, The obesity society, and american society for metabolic & bariatric surgery*
Authors: Mechanick Jeffrey I. , Youdim Adrienne , Jones Daniel B. , Garvey W. Timothy , Hurley Daniel L. , McMahon M. Molly , Heinberg Leslie J. , Kushner Robert , Adams Ted D. , Shikora Scott , Dixon John B. , Brethauer Stacy 
Published: March 2013

AbstractAbstract:The development of these updated guidelines was commissioned by the AACE, TOS, and ASMBS Board of Directors and adheres to the AACE 2010 protocol for standardized production of clinical practice guidelines (CPG). Each recommendation was re‐evaluated and updated based on the evidence and subjective factors per protocol. Examples of expanded topics in this update include: the roles of sleeve gastrectomy, bariatric surgery in patients with type‐2 diabetes, bariatric surgery for patients with mild obesity, copper deficiency, informed consent, and behavioral issues. There are 74 recommendations (of which 56 are revised and 2 are new) in this 2013 update, compared with 164 original recommendations in 2008. There are 403 citations, of which 33 (8.2%) are EL 1, 131 (32.5%) are EL 2, 170 (42.2%) are EL 3, and 69 (17.1%) are EL 4. There is a relatively high proportion (40.4%) of strong (EL 1 and 2) studies, compared with only 16.5% in the 2008 AACE‐TOS‐ASMBS CPG. These updated guidelines reflect recent additions to the evidence base. Bariatric surgery remains a safe and effective intervention for select patients with obesity. A team approach to perioperative care is mandatory with special attention to nutritional and metabolic issues.
DNA methylation of the p66Shc promoter is decreased in placental tissue from women delivering intrauterine growth restricted neonates
Authors: Tzschoppe Anja , Doerr Helmuth , Rascher Wolfgang , Goecke Tamme , Beckmann Matthias , Schild Ralf , Struwe Ellen , Geisel Jürgen , Jung Heike , Dötsch Jörg 
Published: March 2013

ABSTRACTObjectiveThe adaptor protein p66Shc generates mitochondrial reactive oxygen species and translates oxidative signals into apoptosis. We aimed to analyze potential alterations in total methylation and in p66Shc activation in placental tissues from women delivering intrauterine growth restricted neonates (IUGR) versus appropriate for gestational age (AGA) and small for gestational age (SGA) neonates.MethodDNA methylation of the p66Shc promoter and of long interspersed nuclear elements (LINE‐1), as a marker for total methylation, was quantified by automatic pyrosequencing in 15 IUGR, 25 AGA and 15 SGA placentas. Placental gene expression of p66Shc was determined by TaqMan real‐time polymerase chain reaction.ResultsNo significant difference was found for LINE‐1 methylation between IUGR, AGA and SGA newborns. DNA methylation of the p66Shc promoter was significantly decreased in the IUGR compared with the AGA group (p < 0.0001) and the SGA group (p < 0.0001). However, analysis of placental p66Shc gene expression did not show a significant difference between the three groups.ConclusionIt remains speculative if the decreased p66Shc promoter methylation might play a role in the pathophysiology of endothelial dysfunction and cardiovascular disease after IUGR. © 2013 John Wiley & Sons, Ltd.
Long‐term outcomes of children with umbilical vein varix diagnosed prenatally
Authors: Melcer Y. , Ben‐Ami I. , Wiener Y. , Livne A. , Herman A. , Maymon R. 
Published: March 2013

ABSTRACTObjectivesThe current study aims were to assess the long‐term outcomes of children who were diagnosed with umbilical vein varix (UVV) prenatally.MethodsThe study included fetuses with UVV diagnosed in the community between the years 2005 and 2011. They all have been refereed to our Ultrasound Unit for diagnosis' confirmation. This has been conducted locally by a single operator. After delivery, they were matched by gestational age at birth with a set of newborns from a random list. Developmental delay was assessed by telephone interview using a questionnaire based on Ages and Stages Questionnaire. If the child's score (both in the study and control group) was below the cut‐off in one or more domain(s), the families were offered an examination by a child developmental health care team.ResultsThere was no perinatal mortality in both groups. A low‐development score was found in 41.7% (15/36) and 3.7% (4/108) in UVV and control group, respectively (P < 0.05). In 10 out of 15 (67%) children in the UVV group with low scores, formal developmental assessment was performed. Four (40%) were diagnosed having developmental delay. Among the four controls with low score, two families refused additional assessment and one child died. The remaining child was found to have normal development.ConclusionsA possible association between UVV diagnosed prenatally and child developmental delay was found. However, the clinical implications of these findings are still premature; thus, additional studies are needed. © 2013 John Wiley & Sons, Ltd.
Omphalocele in the first trimester: prediction of perinatal outcome
Authors: Tassin Mikaël , Descriaud Céline , Elie Caroline , Debarge Véronique Houfflin , Dumez Yves , Perrotin Franck , Benachi Alexandra 
Published: March 2013

ABSTRACTObjectiveThis study aims to evaluate the perinatal outcomes of fetuses with isolated omphalocele diagnosed before 14 weeks of gestation (WG) and determine whether visceral–abdominal disproportion (ratio between mean omphalocele diameter and transverse abdominal diameter) and omphalocele contents can predict neonatal morbidity.MethodsThis is a retrospective cohort study of omphaloceles diagnosed before 14 WG at three tertiary centers between January 1998 and January 2010. In the group of isolated omphaloceles (i.e., euploid and no other malformation), ratio of visceral–abdominal disproportion and omphalocele contents were evaluated as predictors of perinatal morbidity.ResultsAmong 153 fetal omphaloceles diagnosed before 14 WG, 74 were excluded because of abnormal karyotype or other malformations. Among the 79 isolated fetal omphaloceles, the survival rate at birth was 68% (54/79), with a global morbidity rate of 33% (18/54). Of the live born fetuses, 92.6% (50/54) survived the neonatal period, and 96% (48/50) without long‐term sequelae. There was a significant increase in neonatal morbidity when the ratio of disproportion was greater than 0.8 or when the liver was contained in the omphalocele in the first trimester.ConclusionIn cases of isolated omphalocele in the first trimester, visceral–abdominal disproportion and omphalocele contents predict perinatal morbidity. © 2013 John Wiley & Sons, Ltd.