bluuebunny/crossref_metadata_2025_split huggingface dataset:
Divergent tail and throat ornamentation in the barn swallow across the Japanese islands
Authors: Hasegawa Masaru , Arai Emi 
Published: November 2012

AbstractThe geographic variations in male ornamentation provide insights into how different populations reach a different mean trait value under opposing forces of natural and sexual selection. Although the latitudinal cline of the elongated tail streamer, a sexually selected trait in the European subspecies of the barn swallowHirundo rustica rustica, is a classic example, it has recently been shown that other subspecies of swallows have different targets of sexual selection. Here, we studied the latitudinal cline of ornamentation in the Asian subspecies,H.r.gutturalis, in which not the tail length but the white tail spot and red throat patch are important sexually selected traits. After controlling for covariates, the size of the white tail spot increased with latitude, while the size of the red throat patch decreased with latitude. On the other hand, we could not find any clear pattern regarding the elongated tail streamer, measured as fork depth. The divergent ornamentation across populations could be explained by latitudinal clines of sexually selected advantages of each ornament.
Micellization and Adsorption Behaviors of New Reactive Polymerizable Surfactants Based on Modified Nonyl Phenol Ethoxylates
Authors: Atta Ayman M. , Dyab Amro K. F. , Al‐Lohedan Hamad A. 
Published: November 2012

AbstractThe synthesis and characterization of a series of polymerizable surfactants based on alkyl phenol ethoxylate backbone and carboxylic or anhydride chain ends were investigated. Surface activities of these polymerizable surfactants were investigated to correlate their structure and their performances. The new bifunctional surfmers were prepared by reacting polyoxyethylene 4‐nonyl‐2‐propylene‐phenol nonionic reactive surfactants with maleic anhydride. The chemical structure of the prepared surfactants was characterized by 13C and 1H NMR analyses. The surface activities of the modified polymerizable surfactants were measured from the adsorption isotherm measurements which were determined from the relationship between the concentrations and surface tension of surfactants in aqueous medium at different temperatures. Critical micelle concentration (CMC) values were determined for water soluble surfactants. It was found that CMC decreases with the incorporation of the anhydride and acid groups in the chemical structure of polyoxyethylene 4‐nonyl −2‐propylene‐phenol nonionic surfactant. surface‐active parameters such as area per molecule at the interface (Amin), surface excess concentration (Γmax) and the effectiveness of surface tension reduction (πCMC) were measured from the adsorption isotherms of the modified surfactants. Some thermodynamic data for the adsorption process were calculated and discussed. The data indicated that the new surfmers are more reactive than the simple polyoxyethylene 4‐nonyl‐2‐propylene‐phenol and more adsorbed at interfaces. We have performed a preliminary experiment to explore the emulsification efficiency of the newly synthesized reactive surfactants in equal volume oil–water emulsions. Different emulsion types and stabilities were obtained.
Regulation of GluA1 AMPA Receptor Through PKC Phosphorylation Induced by Free Fatty Acid Derivative HUHS2002
Authors: Nishimoto Takaaki , Kanno Takeshi , Shimizu Tadashi , Tanaka Akito , Nishizaki Tomoyuki 
Published: November 2012

AbstractThe present study investigated the effect of 4‐[4‐(Z)‐hept‐1‐enyl‐phenoxy] butyric acid (HUHS2002), a newly synthesized free fatty acid derivative, on α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole propionic acid (AMPA) receptor responses. HUHS2002 potentiated currents through GluA1 AMPA receptors expressed in Xenopus oocytes in a bell‐shaped concentration (1 nM–1 μM)‐dependent manner, the maximum reaching nearly 140 % of original amplitude at 100 nM. The potentiation was significantly inhibited by GF109203X, an inhibitor of protein kinase C (PKC), but not KN‐93, an inhibitor of Ca2+/calmodulin‐dependent protein kinase II (CaMKII). HUHS2002 had no potentiating effect on currents through mutant GluA1 AMPA receptors with replacement of Ser831, a PKC/CaMKII phosphorylation site, by Ala. In the in situ PKC assay using rat PC‐12 cells, HUHS2002 significantly enhanced PKC activity, that is suppressed by GF109203X. Overall, the results of the present study show that HUHS2002 potentiates GluA1 AMPA receptor responses by activating PKC and phosphorylating the receptors at Ser831, regardless of CaMKII activation and phosphorylation.
Accumulation of 5‐Hydroxymethylfurfural in Oil During Frying of Model Dough
Authors: Göncüoğlu Neslihan , Gökmen Vural 
Published: November 2012

Abstract5‐Hydroxymethylfurfural (HMF), a thermal process contaminant, forms in food during frying as a result of the Maillard reaction and caramelization. Owing to its chemical properties, HMF formed in foods during frying partially transfers into frying oil. This study aimed to investigate the accumulation of HMF in oil during repetitive frying operations. A model dough composed of 25 % of glucose was fried at 160, 170, 180 °C for 50 frying cycles. Apart from total polar compounds (TPC), accumulation of HMF was determined in oils during repetitive frying operations. Increasing frying temperature also increased the amount of HMF formed in dough, and those transferred to oil. Prolonging frying cycles to 150, increasing amount of dough being fried to 100 g and frying time to 10 min caused the TPC content to reach 25 % at the 130th frying cycle at 180 °C. Under the same frying conditions, the concentration of HMF showed a rapid increase at the first 10th frying cycle. Its increase was at a slower rate until the 50th frying cycle reaching a plateau level exceeding 5.0 mg/L. The results revealed that HMF transferred and accumulated in the frying oil during repetitive frying. The HMF concentration exceeding 5.0 mg/L in oil may be considered as an indicator for heavily used frying oil. Correlation between TPC and HMF contents of frying oil showed no linear correlation.
A Juzvinskii addition theorem for finitely generated free group actions
Authors: BOWEN LEWIS , GUTMAN YONATAN 
Published: November 2012

AbstractThe classical Juzvinskii addition theorem states that the entropy of an automorphism of a compact group decomposes along invariant subgroups. Thomas generalized the theorem to a skew-product setting. Using L. Bowen’s f-invariant, we prove the addition theorem for actions of finitely generated free groups on skew-products with compact totally disconnected groups or compact Lie groups (correcting an error in L. Bowen [Nonabelian free group actions: Markov processes, the Abramov–Rohlin formula and Yuzvinskii’s formula. Ergod. Th. & Dynam. Sys.30(6) (2010), 1629–1663]) and discuss examples.
Slow pace of dietary change in Scotland: 2001–9
Authors: Wrieden Wendy L. , Armstrong Julie , Sherriff Andrea , Anderson Annie S. , Barton Karen L. 
Published: November 2012

Monitoring changes in the food and nutrient intake of a nation is important for informing the design and evaluation of policy. Surveys of household food consumption have been carried out annually in the UK since 1940 and, despite some changes over the years 1940–2000, the method used for the Expenditure and Food Survey (Living Costs and Food Survey from 2008) has been fundamentally the same since 2001. Using these surveys an analytical procedure was devised to compare food consumption and nutrient intake in Scotland with the Scottish dietary targets, and monitor change. This method takes into account contributions to composite foods and losses due to food preparation, as well as inedible and edible waste. There were few consistent improvements in consumption of foods or nutrients targeted for change over the period 2001–9. A significant but small increase was seen in mean fruit and vegetable consumption (259 g/d in 2001, 279 g/d in 2009, equating to an increase of less than 3 g/person per year). There was also a significant decrease in the percentage of food energy from SFA (15·5 % in 2001, 15·1 % in 2009) and from non-milk extrinsic sugars (15·5 % in 2001, 14·8 % in 2009), concurrent with a reduction in whole milk consumption and soft drink consumption, respectively. These small changes are encouraging, but highlight the time taken for even modest changes in diet to occur. To achieve a significant impact on the health of the present Scottish population, the improvements in diet will need to be greater and more rapid.
Beneficial effect of genistein on lowering blood pressure and kidney toxicity in fructose-fed hypertensive rats
Authors: Palanisamy Nallasamy , Venkataraman Anuradha C. 
Published: November 2012

The study evaluates the effects of genistein on blood pressure (BP) and ultrastructural changes in kidney of fructose-fed hypertensive rats. Male Wistar rats were fed a diet containing 60 % starch or 60 % fructose as the source of carbohydrate. After 15 d, rats in each dietary group were divided into two groups and were treated with either genistein (1 mg/kg per d) in dimethylsulfoxide (DMSO) or 30 % DMSO alone. BP, pressor mechanisms, protein kinase C-βII (PKC-βII) expression, endothelial NO synthase (eNOS) expression and renal ultrastructural changes were evaluated after 60 d. Fructose-fed rats displayed significant elevation in BP and heart rate. Significant increase in plasma angiotensin-converting enzyme (ACE) activity, alterations in renal lipid profile, nitrite and kallikrein activity, enhanced expression of membrane-associated PKC-βII and decreased expression of eNOS were observed in them. Histology and electron microscopic studies showed structural changes in the kidney. Genistein administration lowered BP, restored ACE, PKC-βII and eNOS expression and preserved renal ultrastructural integrity. These findings demonstrate that genistein has effects on eNOS activity in renal cells, leading to eNOS activation and NO synthesis. These effects could have been mediated by activation of PKC-βII. The observed benefits of genistein make it a promising candidate for therapy of diabetic kidney disease.
Net transfer of nutrients to the duodenum and disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep fed grass/legume combinations
Authors: Keli Abdelhafid , Olmos Genaro , de Vega Antonio , Guada José A. 
Published: November 2012

An experiment was carried out to examine the effect of increasing the proportion of Wimmera ryegrass hay in a lucerne hay-based diet on net transfer of nutrients to the intestine, and on the disappearance ofn-alkanes in the reticulo-rumen and the hindgut of sheep. Following a latin square design, four adult ewes were fed 1:0, 0·33:0·67, 0·67:0·33 and 0:1 proportions of legume and grass. Increasing the proportion of ryegrass in the diet linearly decreased the intake of DM (P= 0·017), organic matter (P= 0·021) and N (P= 0·001). However, neutral-detergent fibre intake was not affected (P= 0·148), nor was its digestibility coefficient (P>0·10). Diet had no effect on duodenal flows of nutrients (P>0·10), although the proportion of N intake (NI) recovered at the duodenum as non-NH3N (NAN) increased linearly withLolium rigidumin the diet (P= 0·002). Full recovery of NI as NAN was achieved at NH3concentrations in the rumen below 110 g/l. Microbial N contribution to NAN varied in a quadratic manner (P< 0·05) with the proportion of grass in the diet, although efficiency of microbial synthesis was not affected (P>0·10). Duodenal recovery of consumedn-alkanes was not affected by diet and was complete for those present in higher concentrations in the forages. Isolated rumen bacteria contained significant amounts ofn-alkanes, contributing to the duodenal flow of these hydrocarbons in variable proportions depending on the diet consumed.
Lower nutritional status and higher food insufficiency in frail older US adults
Authors: Smit Ellen , Winters-Stone Kerrie M. , Loprinzi Paul D. , Tang Alice M. , Crespo Carlos J. 
Published: November 2012

Frailty is a state of decreased physical functioning and a significant complication of ageing. We examined frailty, energy and macronutrient intake, biomarkers of nutritional status and food insufficiency in US older adult (age ≥ 60 years) participants of the Third National Health and Nutrition Examination Survey (n4731). Frailty was defined as meeting ≥ 2 and pre-frailty as meeting one of the following four-item criteria: (1) slow walking; (2) muscular weakness; (3) exhaustion and (4) low physical activity. Intake was assessed by 24 h dietary recall. Food insufficiency was self-reported as ‘sometimes’ or ‘often’ not having enough food to eat. Analyses were adjusted for sex, race, age, smoking, education, income, BMI, other co-morbid conditions and complex survey design. Prevalence of frailty was highest among people who were obese (20·8 %), followed by overweight (18·4 %), normal weight (16·1 %) and lowest among people who were underweight (13·8 %). Independent of BMI, daily energy intake was lowest in people who were frail, followed by pre-frail and highest in people who were not frail (6648 (se130), 6966 (se79) and 7280 (se84) kJ, respectively,P< 0·01). Energy-adjusted macronutrient intakes were similar in people with and without frailty. Frail (adjusted OR (AOR) 4·7; 95 % CI 1·7, 12·7) and pre-frail (AOR 2·1; 95 % CI 0·8, 5·8) people were more likely to report being food insufficient than not frail people. Serum albumin, carotenoids and Se levels were lower in frail adults than not frail adults. Research is needed on targeted interventions to improve nutritional status and food insufficiency among frail older adults, while not necessarily increasing BMI.
Infection, growth and maintenance ofWolbachia pipientisin clonal and non-clonalAedes albopictuscell cultures
Authors: Khoo C.C.H. , Venard C.M.P. , Fu Y. , Mercer D.R. , Dobson S.L. 
Published: November 2012

AbstractInsect cell lines provide usefulin vitromodels for studying biological systems, including interactions between mosquitoes and obligate intracellular endosymbionts such asWolbachia pipientis. TheAedes albopictusAa23 cell line was the first cell line developed to allow examination ofWolbachiainfections. However,Wolbachiastudies using Aa23 can be complicated by the presence of different cell types in the cell line and the substantial temporal variation in infection level. Two approaches were examined to ameliorate infection variability. In the first approach, multiple Aa23 passaging regimes were tested for an effect on infection variability. Fluorescencein situhybridization (FISH) staining was used to characterizeWolbachiainfection level over time. The results demonstrate an impact of passaging method onWolbachiainfection level, with some methods resulting in loss of infection. None of the passaging methods succeeded in effectively mitigating infection level variation. In a second approach, the clonal C7-10A. albopictuscell line was infected withWolbachiafrom Aa23 cells andDrosophila simulans(Riverside), resulting in cell lines designated C7-10B and C7-10R, respectively. Characterization via FISH staining showed greater stability and uniformity ofWolbachiainfection in C7-10R relative to the infection in C7-10B. Characterization of the Aa23, C7-10B and C7-10R lines is discussed as a tool for the study ofWolbachia-host cell interactions.
Efficiencies of nitrogen fertilizers for winter cereal production, with implications for greenhouse gas intensities of grain
Authors: SYLVESTER-BRADLEY R. , KINDRED D. R. , WYNN S. C. , THORMAN R. E. , SMITH K. E. 
Published: November 2012

SUMMARYFertilizer nitrogen (N) accounts for the majority of the greenhouse gas (GHG) emissions associated with intensive wheat production, and the form of fertilizer N affects these emissions. Differences in manufacturing emissions (as represented in the current carbon accounting methodologies) tend to favour urea, even when using the best available manufacturing technologies (BAT), whereas differences in fertilizer N efficiency and emissions of ammonia tend to favour ammonium nitrate (AN). To resolve these differences, data from 47 experiments in two large UK studies conducted from 1982 to 1987 and from 2003 to 2005 were reanalysed, showing that on average urea efficiency was 0·9 of AN (although mean ammonia emissions in 10 subsidiary experiments indicated an efficiency difference of 0·2); treating urea with a urease inhibitor (TU; AGROTAIN®, active ingredient N-(n-butyl) thiophosphoric triamide (n-BTPT)) brought its efficiency almost in line with AN; however, a significantly greater mean optimum N amount for TU (+0·1 of AN) was not fully explained. A standard response function relating wheat yield to applied AN was modified for degrees of relative inefficiency, thus enabling yields and GHG intensities (kg CO2e/tonne (t) grain) to be calculated using a PAS2050 compatible model for GHG emissions for any N amount of any N form. With AN manufactured by average European technology (AET), the estimated GHG intensity of wheat producing 8 t/ha was 451 kg/t; whereas with urea or TU made by AET it was 0.87–0.99 or 0.84–0.86 of this respectively. Using BAT for fertilizer manufacture, the grain's GHG intensity with AN and TU was 368 kg/t, but was 1·03–1·17 of this with untreated urea. The range of effects on GHG intensities arose mainly from remaining uncertainties in the inefficiencies of the N forms. Generally, economic margins and GHG intensities were not much affected by adjustments in N use for relative inefficiencies or different prices of urea-based fertilizers compared with AN. Overall, TU appeared to provide the best combination of economic performance and GHG intensity, unless the price for N as TU exceeded that for N as AN.
Chymotrypsin selectively digests β-lactoglobulin in whey protein isolate away from enzyme optimal conditions: Potential for native α-lactalbumin purification
Authors: Lisak Katarina , Toro-Sierra Jose , Kulozik Ulrich , Božanić Rajka , Cheison Seronei Chelulei 
Published: November 2012

The present study examines the resistance of the α-lactalbumin to α-chymotrypsin (EC 3.4.21.1) digestion under various experimental conditions. Whey protein isolate (WPI) was hydrolysed using randomised hydrolysis conditions (5 and 10% of WPI; pH 7·0, 7·8 and 8·5; temperature 25, 37 and 50 °C; enzyme-to-substrate ratio, E/S, of 0·1%, 0·5 and 1%). Reversed-phase high performance liquid chromatography (RP-HPLC) was used to analyse residual proteins. Heat, pH adjustment and two inhibitors (Bowman–Birk inhibitor and trypsin inhibitor from chicken egg white) were used to stop the enzyme reaction. While operating outside of the enzyme optimum it was observed that at pH 8·5 selective hydrolysis of β-lactoglobulin was improved because of a dimer-to-monomer transition while α-la remained relatively resistant. The best conditions for the recovery of native and pure α-la were at 25 °C, pH 8·5, 1% E/S ratio, 5% WPI (w/v) while the enzyme was inhibited using Bowman–Birk inhibitor with around 81% of original α-la in WPI was recovered with no more β-lg. Operating conditions for hydrolysis away from the chymotrypsin optimum conditions offers a great potential for selective WPI hydrolysis, and removal, of β-lg with production of whey protein concentrates containing low or no β-lg and pure native α-la. This method also offers the possibility for production of β-lg-depleted milk products for sensitive populations.
Ultrastructural and molecular studies of Microgemma carolinus n. sp. (Microsporidia), a parasite of the fish Trachinotus carolinus (Carangidae) in Southern Brazil
Authors: CASAL G. , MATOS E. , GARCIA P. , AL-QURAISHY S. , AZEVEDO C. 
Published: November 2012

SUMMARYA new species of Microsporidia Microgemma carolinus n. sp. found in the marine teleost Trachinotus carolinus collected in Florianópolis, Brazil was described based on light, ultrastructural and phylogenetic studies. This parasite developed in the liver forming whitish xenomas that contained different developmental stages with monokaryotic nuclei. The periphery of the xenoma presented some vacuolization and possessed several small projections in the membrane. The mature spores, measuring 3·8 ± 0·4 μm in length and 2·4 ± 0·4 μm in width, were slightly pyriform to ellipsoidal and had rounded ends. The polaroplast was bipartite and the isofilar polar filament was coiled with 8 – 9 turns in a single or double row at the posterior end of the spore. The nucleus was voluminous and in a central position, measuring ∼0·9 μm in diameter. A large posterior vacuole appeared as a pale area, occupying about a third of the spore length. The SSU rRNA gene was sequenced and analysed using maximum parsimony, maximum likelihood and neighbour-joining methods. This study allowed us to conclude that this was a new species of the genus Microgemma, being the first description of this genus from among South America fauna.
Genocide and Discrimination: Lessons to Be Learnt from Discrimination Law
Authors: AMBRUS MONIKA 
Published: November 2012

AbstractA survey of the case law of the ICTY and ICTR reveals inconsistencies and conceptual discrepancies in the approach to genocide cases, in particular with respect to the cornerstones of such cases – the identification of the protected group and its members – resulting in different levels of protection against genocide. A review model might help to address these issues, which are, arguably, preconditions of the legitimacy of these courts and of the effective enforcement of the prohibition of genocide. Given the close relationship between genocide and discrimination, this review model might be built on the ‘building blocks’ of discrimination law. The purpose of this contribution is twofold: (1) by unfolding the relationship between direct discrimination and genocide to devise a model of review, and (2) to analyse the consequences of the consistent application of this model for the identification of the protected groups and their members.
The Ne Bis in Idem Principle in the Interpretation of European Courts: Towards Uniform Interpretation
Authors: NEAGU NOREL 
Published: November 2012

AbstractAs a result of the extension of the jurisdiction of the Court of Justice of the European Union over the former third pillar (Police and Judicial Cooperation in Criminal Matters), several cases were referred to the Court for interpretation, inter alia, of the dispositions of the Schengen Convention dealing with criminal matters, especially the ne bis in idem principle. This principle was also addressed in the case law of the European Court of Human Rights, the Inter-American Court of Human Rights, and the Supreme Court of the United States. While addressing the problem at international level, this article focuses principally on the case law of the Court of Justice of the European Union and the European Court of Human Rights in the field of the ne bis in idem principle, concisely presenting the legal framework, findings of the Courts, and some conclusions on the interpretation of the principle. The study also analyses the absence of uniformity in interpretation and the use of different criteria in addressing identical situations by different courts, or even by the same court, concluding on a (seemingly) fortunate approximation in interpretation at European level.