bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of vertical forest stratification on precipitation material redistribution and ecosystem health of Pinus massoniana in the Three Gorges Reservoir area of China
Authors: Yangyang Yan , Huang Zhihua , Kun Yan , Qin Liu 
Published: June 2024

Abstract

Vertical stratification of forest plays important roles in the local material balance and in maintaining forest health by distributing and redistributing precipitation materials through adsorption, fixation, and release. Differences in runoff nutrient concentrations among vertical layers are closely related to vertical stratification (factors such as the trunk, canopy, forest litter, and soil physical and chemical properties). Long-term forest observations revealed significant spatial differences in Masson pine (Pinus massoniana Lamb.) forests in the Three Gorges Reservoir area. Masson pine forests on downslopes were characterized by a dense canopy, green needles, and rich forest vegetation, while those on upslopes were characterized by low vegetation cover, dead trees, and decreases in the tree height, diameter at breast height, and volume per plant with increasing slope. By analyzing the soil at different sites, we found that the pH of the forest land soil differed significantly among different slope positions. Soil on upper slopes was significantly more acidic than soil on lower slopes, indicating that acidic substances were intercepted by filtration through the broad litter layer and the soil surface layer. This filtration process resulted in a normal rhizosphere environment suitable for the absorption of nutrients by vegetation on the lower slopes. In this way, downhill sites provided a good microenvironment for the growth of Masson pine and other vegetation. Our results show that direct contact between needles and acid rain was not the main cause of root death. Instead, the redistribution of rainfall substances by forest spatial stratification caused changes in the soil microenvironment, which inhibited the absorption of nutrients by the roots of Masson Pine and the growth of understory plants in Masson pine forests on upper slopes. These findings emphasize that increasing land cover with forests with vertical structural stratification plays an important role in woodland material redistribution and forest conservation.
Identifying potential ferroptosis-related biomarkers and therapeutic targets for IPAH by integrative bioinformatics approaches
Authors: Tan Mingliang , Qian Tao , Jiang Wei , Yuan Haoyong , Lu Ting , Yin Ni , Wu Zhongshi , Huang Can 
Published: June 2024

Abstract

Background: Idiopathic pulmonary arterial hypertension (IPAH), a rare and devastating pulmonary vascular disorder, is characterized by cellular proliferation and vascular remodeling. Although previous studies have underscored that ferroptosis, an iron-dependent cell death process, plays an important regulatory role in pulmonary artery hypertension, its role remains understudied.
Methods: Gene expression profiles were downloaded from the Gene Expression Omnibus. Differentially expressed genes (DEGs) were screened using R software and intersected with a ferroptosis database (FerrDb V1) to identify ferroptosis-related DEGs. GO and KEGG analyses were performed to explore biological functions and potential pathways. LASSO and SVM‐RFE algorithms were used to identify optimal gene biomarkers for IPAH. GSVA and GSEA were conducted to explore biological functions and potential pathways associated with these biomarkers. The CIBESORT software was employed to predict immune genes and functions.
Results: Of 237 ferroptosis-related genes (FRGs), 27 differentially expressed FRGs (DE-FRGs) showed significant differences between IPAH and normal samples in GSE48149, with 15 downregulated and 12 upregulated genes. Six DE‐FRGs, including KEAP1, TNFAIP3, MEG3, NFS1, PRDX1, and BEX1, were identified as predictive diagnostic genes for IPAH. Among these DE-FRGs, PRDX1 and TNFAIP3 were the most promising diagnostic genes for IPAH and may play a corresponding role in IPAH by participating in the cell cycle, lysosomes, immune response, vascular smooth muscle contraction, and various diseases. CIBERSORT analysis revealed a positive correlation between neutrophils and TNFAIP3, whereas macrophages M0 exhibited a negative correlation with PRDX1.
Conclusions: Our analysis revealed six key genes in IPAH: KEAP1, TNFAIP3, MEG3, NFS1, PRDX1, and BEX1. PRDX1 and TNFAIP3, showing consistent expression across datasets, emerge as potential biomarkers and targets for IPAH.
Investigation into the Effect and Mechanism of Temozolomide (TMZ) Inducing Ferroptosis to Inhibit Glioma Cell Proliferation
Authors: Yu Jianglong , Aisha Abudula , Jing Xiepan , Yang Yan , Luan Xinping , Zhang Xu 
Published: June 2024

Abstract

Although temozolomide (TMZ) is a first-line chemotherapeutic agent for treating malignant gliomas, its mechanisms of action remain incompletely understood, and primary or acquired resistance is prevalent in certain patients, severely limiting its clinical efficacy. Therefore, elucidating the molecular mechanisms underlying TMZ's mode of action is crucial for improving its therapeutic efficacy.
This study aimed to delineate the effects of TMZ on glioma cells and the molecular mechanisms underlying its induction of ferroptosis. Using human glioma cell lines U87 and U251 as models, we systematically evaluated the impact of TMZ on cell proliferation, cell cycle, DNA integrity, and oxidative stress status through experiments including CCK-8, flow cytometry, and immunofluorescence. Further mechanistic investigations utilizing gene silencing and small molecule inducers elucidated the pivotal role of glutathione peroxidase 4 (GPX4) in the ferroptosis process induced by TMZ.
Results demonstrated that TMZ inhibited glioma cell proliferation, induced cell cycle arrest and DNA damage, while concurrently promoting reactive oxygen species (ROS) accumulation and glutathione (GSH) depletion, along with downregulation of GPX4 expression. Ferroptosis inducers Erastin or RSL3 significantly augmented TMZ's cytotoxicity, exacerbating lipid peroxidation and GSH consumption. GPX4 gene silencing also enhanced TMZ's cytotoxic effects and synergistically interacted with Erastin to promote ferroptosis. Furthermore, knockdown of the GPX4 downstream target ATP-binding cassette transporter ABCC1 increased sensitivity to TMZ. This study unveiled a novel mechanism by which TMZ induces ferroptosis via the GPX4/ABCC1 pathway, providing important insights into understanding its molecular modes of action and improving clinical therapeutic efficacy.
Aviation in Transition: On the Necessity of Recycling Highly Stressed Primary Structures Post End-of-Life and Appropriate Disassembly Technologies - Part 02 - Mathematical Considerations of the Separation Process
Authors: Kreikemeier Janko , Schwieger Hans Helge 
Published: June 2024

Abstract

In the current effort towards sustainability, the aviation industry faces challenges in repurposing carbon fiber reinforced plastic (CFRP) components effectively. Traditional "downcycling" methods fail to maintain CFRP integrity, as they involve cutting load-bearing fibers and re-embedding them with new polymers, which leads to fragmentation and loss of properties.
Innovative solutions like non-destructive disassembly or dismanteling offer precise separation without compromising fiber integrity. This method allows for the direct reuse of materials in similar or new applications and highlights the importance of advanced recycling technologies for fiber-reinforced plastics.
Finite element method (FEM) analysis shows the basic separation mechanism functions without damaging the base material in simulated shell structures. Safety margins are within acceptable ranges, and additional damping forces enhance process safety. Thinner wedges are recommended as they generate lower loads, exhibit less dependency on plate stiffness, and require shorter crack lengths. Fracture risk is influenced by specific parameters.
Critical parameters must be determined during structure development, as they cannot be varied in existing components without design considerations for recyclability. However, green inputs can be directly modified to improve the process. Targeted force applications, such as pressing at the crack tip and relieving force at the wedge contact point, reduce structural load. A cable force mechanism, supported by a lateral roller, effectively relieves wedge contact force.
In summary, the wedge separation process is suitable for non-destructive disassembly of stiffeners, representing a promising approach for developing practical test stands.
Associations of Early-Life Exposure indoor environment with preschool children allergic rhinitis: A case-control study in Haikou city
Authors: Wu Qisheng , Fan Shiheng , Zhou Bingxian , Su Zhuna , Lu Chunyu , Zhang Nengde , Peng Jianye , Yu Dee , Zhang Jing 
Published: June 2024

Abstract

Objective: The study aimed to examine the association between allergic rhinitis and early-life indoor environmental exposure in preschool children in Haikou City, providing a scientific theoretical basis for the prevention of allergic rhinitis in this population.
Methods: In January 2021, we conducted the China Child and Home Health (CCHH) study using a case-control approach with 1090 preschool children in Haikou City, Hainan Province, including 218 cases and 872 controls. The study included baseline demographic information, residential and indoor environmental characteristics, living habits, and children's allergies. Two-level logistical regression analysis was used to examine the association of Early-Life Exposure indoor environment with preschool children allergic rhinitis in terms of odds ratio (OR) and 95% confidence interval (CI).
Results: Mother with an educational level of university or above (OR=1.443, 95% CI=1.024~2.035), having parental atopy (OR=4.604, 95% CI=3.339~6.349), damages or molds found in the mother's residence prior to pregnancy (OR=1.613, 95% CI=1.044~2.493), and passive smoking of the mother during pregnancy (OR=1.833, 95% CI=1.282~2.623) were risk factors for allergic rhinitis in preschool children.
Conclusions: In this case-control study, we found significant associations between parental atopy, mother's education, and indoor environment in early life with the development of allergic rhinitis in preschool children. Therefore, prophylactic measures should be actively implemented in preschool children with allergic parental atopy, as well as preventing the mother's exposure to harmful factors in the indoor environment before and during pregnancy.
Deep Canonically Correlated Denoising Autoencoders for Signature Verification Purpose
Authors: Zhao Xingbiao , Chen Enze , Yuan Panli , Yuan Limengzi , Zheng Yuchen 
Published: June 2024

Abstract

With the rapid development of modern Internet of Things (IoT) technology, thehandwritten signature verification system becomes a typical Human-ComputerInteraction (HCI) application, which is often applied to many authorization documents with legal uses. To build a high-performance verification system, featureextraction is one of the most crucial steps. However, the generalization abilityof traditional deep learning-based feature extractors is not always satisfactorysince most feature extractors are only suitable for source signature datasets.In this paper, to improve the generalization ability of existing deep learning-based feature extractors for the offline signature verification task, we proposea novel multi-view learning-based framework, named Deep Canonically Corre-lated Denoising Autoencoders (DCCDAE). Specifically, the DCCDAE generatesthe joint features as the final features based on original deep learning-basedfeatures and another noisy view of them by optimizing the Canonically Cor-related Analysis (CCA) objective and minimizing the reconstruction error oforiginal features. Extensive experiments and discussions on four publicly avail-able datasets, GPDS, CEDAR, MCYT-75, and PUC-PR demonstrate that theproposed DCCDAE can improve the generalization ability of the original deeplearning-based feature extractor and achieve the state-of-the-art performancecompared with other offline signature verification systems. The code is availableat https://github.com/star0511/DCCDAE
First molecular evidence of Angiostrongylus vasorum in an African Golden wolf (Canis lupaster) in Algeria
Authors: Mechouck Noureddine , Deak Georgiana , Ionică Angela Monica , Toma Corina Gina , Negoescu Andrada Gabriela , Taulescu Marian , Bouslama Zihad , Mihalca Andrei Daniel 
Published: June 2024

Abstract

Background
Angiostrongylus vasorum, the “French heartworm” is a nematode belonging to the Metastrongyloidea superfamily. This parasite was first identified in Toulouse, France by Serres in 1853 infecting the pulmonary arteries and the right side of the heart of a Pointer dog. There is no report regarding this nematode in Algeria. This report aims to investigate the presence of lungworms among different mammal species in different Algerian regions.
Materials and methods
Between February 2022 and September 2023, 47 road-killed animals were collected from six departments in Algeria. All carcasses were subjected to a full parasitological investigation, and lung tissues were preserved in 10% buffered formalin and 70% ethanol. All collected samples were subjected to histology and PCR for lungworm identification.
Results
In a single golden African wolf (Canis lupaster) collected from Constantine, the histological examination revealed the presence of nematode eggs and larvae in the alveolar space and the interstitium-associated chronic obstructive vascular changes. The molecular identification confirmed the presence of Angiostrongylus vasorum. All the other animals were negative for lungworms.
Conclusion
To the best of our knowledge, this is the first report of A. vasorum infecting a golden African wolf (Canis lupaster), reporting a new host association, highlighting the importance of further studies to update the geographical distribution and its epidemiology across Algeria.
Time-lapse Image Super-resolution Neural Network with Reliable
Confidence Quantification for Optical Microscopy
Authors: Dai Qionghai , Qiao Chang , Liu Shuran , Wang Yuwang , Xu Wencong , Geng Xiaohan , Jiang Tao , Zhang Jingyu , Meng Quan , Qiao Hui , Li Dong 
Published: June 2024

Abstract

Single image super-resolution (SISR) neural networks for optical microscopy have shown great capability to directly transform a low-resolution (LR) image into its super-resolution (SR) counterpart, enabling low-cost long-term live-cell SR imaging. However, when processing time-lapse data, current SISR models failed to exploit the important temporal dependencies between neighbor frames, often resulting in temporally inconsistent outputs. Besides, SISR models are subject to inference uncertainty that is hard to accurately quantify, therefore it is difficult to determine to what extend can we trust the inferred SR images. Here, we first build a large-scale, high-quality fluorescence microscopy dataset for the time-lapse image super-resolution (TISR) task, and conducted a comprehensive evaluation on two essential components of TISR neural networks, i.e., propagation and alignment. Second, we devised a deformable phase-space alignment (DPA) based TISR neural network (DPA-TISR), which adaptively enhances the cross-frame alignment in the phase domain and outperforms existing state-of-the-art SISR and TISR models. Third, we combined the Bayesian training scheme and Monte Carlo dropout with DPA-TISR, developing Bayesian DPA-TISR, and designed an expected calibration error (ECE) minimization framework to obtain a well-calibrated confidence map along with each output SR image, which reliably implicates potential inference errors. We demonstrate the unique characteristics of Bayesian DPA-TISR underlie the ultralong-term live-cell SR imaging capability with high spatial fidelity, superb temporal consistency, and accurate confidence quantification on a wide variety of bioprocesses.
­­­­­Investigating the Loyalty of Customers of Dairy Products in Chain Stores Using Data Mining
Authors: Faghihi Alishahram , Borimnejad Vali , Saberikamarposhti Morteza , Rahimibadr Bita 
Published: June 2024

Abstract

This study investigated the loyalty of dairy product customers in chain stores using data mining and investigated the effective factors through logit regression. The statistics used in this study, including the number and amount of purchases by customers, the purchased brand of dairy product, the time and date of purchase, and the available demographic information, were obtained from the convenience store data warehouse daily from the beginning of March 2019 to April 2020. The total number of customers surveyed in the study was 18,232 people. Using the K-means method, customers were clustered. According to the results of the final scenario of 3 clusters, 91% of the active customers were in the first and second clusters. Based on the results, the silhouette coefficient of the third cluster was selected as the superior cluster. According to the results of the recency, frequency, monetary, and continuity (RFMC) model, 50% of the active customers could be grouped according to the leading model. Therefore, the model coverage was appropriate. According to the results of the logit model, older age, access to parking and facilities, quality of service and staff behavior, offering traditional products, and variety of dairy products҆ brands increased the likelihood of customers’ ҆ presence in the loyal category, and being married or male and having a higher educational level increased the likelihood of customers’ ҆ presence in lower loyalty ranks.
Rules and regulations
Authors: Cherqui Frédéric , van Duin Bert , Hernandez Nathalie , Kerres Karsten , Roghani Bardia , Tscheikner-Gratl Franz 
Published: June 2024

Abstract
This chapter covers the legal framework and technical regulations that must or should be observed for the strategic asset management (AM) of urban drainage systems over the entire life cycle (planning, construction, maintenance, and dismantling). A distinction is made between rules and regulations that deal with network management in general (strategic level) and rules and regulations that address the management of individual network components and/or certain activities (e.g., CCTV-inspection and condition assessment of reaches or stormwater basins). These activities are subsumed under ‘operative level’.
It should be noted that both the legal framework and the applicable technical regulations vary widely from region to region. In some cases, different regulations apply even in different provinces or federal states of a country. Against this background, only case studies can (such as regulations that apply to Germany, France, Colombia or Canada) and will be presented in this chapter.
It is thus made clear that AM in the sense of ISO 55000 to 55002 (AM) enables a structured approach to a multi-layered field of tasks. In this way, goals and conflicting goals can be identified and prioritized at various levels and, in conjunction with the continuous improvement process in accordance with ISO 9000 and 9001 (Quality Management Systems), efficient ways can be found to achieve these goals.
Investigate the condition of an asset
Authors: Boogaard F. , Cherqui F. , Clemens-Meyer F.H.L.R. , Lepot M.L. , Shepherd W. , van der Valk M. 
Published: June 2024

Abstract
Monitoring the technical state of an urban drainage (UD) system is at the core of asset management, the deployment of visual inspection technology (either using direct visual access for inspection of applying photo and/or video cameras) was and has remained the main method of gathering information on the technical state. Despite some known fundamental shortcomings visual inspection is expected to remain the main source of information for inspection for the foreseeable future. This chapter discusses the virtues of visual inspection but also provides insight into other technologies that have been tried and/or deployed on a more limited scale but do offer access to more and more exact information when compared to the visual methods. Although not much experience is available, inspection techniques for nature-based solutions will be discussed as well.
Operation, maintenance and rehabilitation techniques
Authors: Bahrami Mahdi , Boogaard Floris , Bosseler Bert , Cherqui Frédéric , van Duin Bert , Funke Fabian , Goerke Marcel , Kelly-Hooper Francine , Kleidorfer Manfred , Moglia Magnus , Muthanna Tone Merete , Oberascher Martin , Tscheikner-Gratl Franz , van der Valk Martijn , van der Valk Ferry 
Published: June 2024

Abstract
Urban drainage operation, management and rehabilitation can be divided into two distinct segments: traditional grey infrastructures (i.e. pipes and associated components) and green infrastructures. For piped systems this boils down to maintaining the operational safety, stability and tightness of the sewers and special structures. However, this chapter provides an overview on both realms and highlights that, while there is a lot of standardization for grey infrastructures, the knowledge on green ones is much more fractured. They are often composed of both engineered and natural elements such as pipes, flow control systems, vegetation, micro-organisms in the soil or growing media, and also deliver a broad range of beneficial services to our communities and their inhabitants. Existing terminology for pipe networks is adapted by defining a similar distinction for green infrastructures based on the severity of the necessary actions. There will be no focus on other special structures and machinery. Adopting these distinctions, this chapter consists of three parts: (1) pipe network operation and maintenance (O&M), (2) structural rehabilitation of pipe networks and the connected manholes and (3) green infrastructure rehabilitation including O&M focusing on some examples. Consequently, this chapter can be used as guidance on available technologies, existing guidelines and research gaps.
Implementing IWRM approaches with climate-change adaptation on riverside slum upgrading, Indonesia
Authors: Wicaksono Andie Arif , Suprapti Atiek , Sunarti S. , Ariyanti Vicky 
Published: June 2024

ABSTRACT

Characterized by limited access to basic services, slums are particularly vulnerable to the impacts of climate change. The Integrated Water Resources Management (IWRM) approach plays a vital role in addressing these challenges by improving urban resilience. This paper discusses the implementation of the IWRM approach with a climate-change adaptation (CCA) outlook on riverside slum upgrading in Indonesia using the qualitative methodology and a case-study approach. These implementations are analyzed in two layers, at the river-basin and the kampung level. The study found that a multilayered continuation of policy is required to plan the whole-basin water resources management, while the practices at the kampung level require scaling down and contextualization for specific locations. The involvement of multisector stakeholders collaborating with communities at different levels and secured land-tenure status are crucial for success. Different strategies linking water resources management in urban riverside slums with prioritization in addressing hazard control above other pillars of IWRM are a prerequisite for a safe settlement. The paper also highlights the need for more effective implementation, the importance of bridging the gaps between policy orientation and management, and implementations at all levels to ensure success.