bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the morphology of Sus scrofa domesticus oocyte-cumulus complexes exposed to low and ultra-low temperatures
Authors: Starikova D. A. , Kuzmina T. I. 
Published: August 2024

Relevance. The priority task of reproductive technologies in animal husbandry is the development of effective storage protocols for female gametes. One way to preserve gametes is vitrification, which reduces damage to intracellular organelles by increasing viscosity upon cooling and minimizing crystallization.Methods. Various additives to cryoprotectants are used for optimization. In our work, titanium tetrapolyethylene glycolate was used in a 10-fold molar excess of polyethylene glycol (TTPEG*10PEG), the nature of the effect of which on cells was studied during short-term storage at low (5 °С) and ultra-low temperatures (-196 °С).The purpose of the study is a comprehensive analysis of the morphology of gametes and somatic cells (cumulus) of ovarian follicles of pigs after exposure to low and ultra-low temperatures (vitrification) when included in the TTPEG*10PEG low-temperature storage and vitrification protocol.Results. Exposure to TTPEG*10PEG at 5 °С caused an increase in the level of oocytes with subsequent morphological degenerations in points with control (from 13% to 5%, р = 0.005). After vitrification with TTPEG*10PEG: the level of gametes with low cumulus cells expansion increased to 35% in terms of the proportion of control gametes (23%); the proportion of denudated gametes was reduced to 50% compared to the control 65% (р < 0.05); the level of morphologically degenerated gametes corresponded to that of native oocytes (8% each) and was lower than in the control (17%), р < 0.005. The obtained data indicate the protective and cryoprotective effects of TTPEG*10PEG at a concentration of 2%, which suggests the possibility of its use in the technology of intraovarian vitrification of female reproductive cells.
Identification of single nucleotide polymorphisms in BMP-2 gene and their associations with productive traits in Rainbow Trout
Authors: Tyshchenko V. I. , Shcherbakov Yu. S. , Nikolaeva O. A. , Terletsky V. P. 
Published: August 2024

Relevance. Aquaculture is occupying an increasingly important place in fish farming, as it allows obtaining more products per unit area. In recent years, special attention has been paid to the development of new breeds of Rainbow Trout in order to obtain fish forms adapted to aquaculture conditions. One of the significant breeding achievements is the Ropshinsky golden trout, characterized by attractive scale coloration. Taking into account the emergence of such forms of fish, research to identify genetic characteristics is of particular relevance. The BMP-2 gene is an important determinant of fish growth and development, so a comprehensive study of its structure will allow us to identify the most favorable genotypes in producers for their use in further selection and breeding.Results. The aim of the work is to identify single nucleotide polymorphisms (SNPs) in the studied gene and link their presence in parental forms with their productive traits. In particular, after sequencing, several SNPs were identified in different positions both in the intron and exon regions of the gene. The frequencies of occurrence of genotypes in parents were calculated and a number of quantitative traits were measured in the offspring numbered of 50 individuals from each crossing pair. In some cases, it was possible to establish statistically significant associations between the identified SNPs and productive traits in females and males — the parents of the resulting offspring. In females, an association was established between head length and SNP in one gene region, and in males, an association of another polymorphism with ejaculate volume has been demonstrated.
Germination, survival and preservation of tomatoes when using the technological method of grafting in protected soil
Authors: Voronkova I. R. , Rzayeva V. V. 
Published: August 2024

The article discusses the use of technological graftingand its effect on the survival and preservation of tomato plants, and also provides the results of research on seed germination.The purpose of the research is to evaluate the effect of technological inoculation on the survival and preservation of tomato hybrids in protected soil conditions of the Tyumen region. The cultivation of tomatoes at the Narimanovsky municipal plant is carried out using Dutch technology, which has proven its right to be used in the conditions of the sharply continental climate of Siberia and the possibility of creating break-even production based on them. Studies aimed at studying the reception of tomato grafting on stable rootstocks seem timely and promising not only in our country, but also abroad. Scientific research was conducted from 2018 to 2022.The paper presents recommendations for growing tomato hybrids in protected soil as a grafted crop. The advantages of grafted plants are that the graft may not have all types of resistance, and some of them are added at the expense of the rootstock, which is relevant and has not been studied in the conditions of the closed ground of the Tyumen region. Grafting has a complex and prolonged effect on a number of physiological parameters. In the course of research, it was found that growing tomatoes usinggrafting in low-volume hydroponics conditions makes it possible to increase the safety of plants by the end of the turnover.
The effect of bioactive film on the shelf life of bread
Authors: Zinina O. V. , Vishnyakova E. A. , Rebezov M. B. 
Published: August 2024

Relevance. The development of bioactive films is currently relevant due to the need to replace synthetic polymers used in food packaging materials. However, the established positive properties of the films themselves do not always guarantee the predicted effect and do not ensure the preservation of food products during storage. In this regard, for the recommendation of biodegradable films of a specific composition for use as packaging material for a particular type of product, experimental confirmation is required.Methods. The objects of study are samples of wheat bread packaged in alginate films and in polyethylene film as a control. For bread samples after baking and cooling, as well as after 1, and 3 days of storage, sensory characteristics, color characteristics, microbiological indicators, and mass losses were determined.Results. The conducted tests of bread samples revealed the positive effect of the bioactive film based on sodium alginate with the introduction of protein hydrolysate on reducing the growth of bacteria and molds. The research results showed that in alginate films, there is an intensive loss of bread mass — up to 19.27 ± 0.51% (p ≤ 0.05) after 3 days of storage in the film without protein hydrolysate, which is more significant compared to the bread sample in polyethylene film (9.12 ± 0.32%). At the same time, microbiological indicators (total microbial count and mold count) remained more stable in bread samples in alginate films. Thus, the results of microbiological studies confirmed the known data on the antimicrobial properties of protein hydrolysates in the composition of bioactive films. However, when choosing films for bread packaging, it is necessary to consider their vapor permeability to avoid moisture losses during storage..Results. The results of the study showed the need to review the practice of using manure (manure) processing technologies to reduce greenhouse gas emissions
Diagnosis of avian salmonellosis using PCR kits of different manufacturers
Authors: Ruzina A. V. 
Published: August 2024

Relevance. The problem in timely detection of this infection is that poultry contaminated with salmonellae may not show clinical signs of the disease. Polymerase chain reaction in the diagnosis of avian salmonellosis is designed for direct detection and identification of pathogenic agents, especially in cases where their detection is difficult by other diagnostic methods. Advantages of PCR tests allow to successfully use this method in veterinary laboratory practice. The aim of this work is to conduct comparative tests of diagnostic PCR kits of different manufacturers in the diagnosis of avian salmonellosis.Methods. To conduct comparative studies of pathological material for the presence of the DNA of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium by molecular biological method, three commercial kits of domestic and imported production for isolation and detection of Salmonella DNA by PCR were used. Determination of the possibility of using membrane glass fiber materials, in the form of cards for taking dry samples of pathogenic material, for subsequent PCR was carried out in comparative tests with parallel studies of biomaterial delivered to the laboratory in frozen form.Results. Comparative studies of pathological material for the presence of Salmonella spp. DNA using three commercial kits for the isolation and detection of Salmonella DNA by polymerase chain reaction, imported and domestic production were carried out. It was found that when comparing the results obtained using kits of different manufacturers for the presence of Salmonella spp. DNA, comparable results were observed. The study was performed within the framework of the state assignment No. FGUG-2022-0009 of (FSC VIEV).
The influence of boars of producers of various breeding on the economic indicators of the use of sows
Authors: Belookov A. A. , Belookova O. V. , Rebezov M. B. 
Published: August 2024

Relevance. Interbreeding or hybridization is an important factor in increasing pig productivity. As a result of the heterosis effect, the multiplicity of sows increases, the safety of offspring increases, the growth rate of young animals improves, the quality of meat improves and, as a result, the profitability of the pig industry increases.Methods. The paper evaluates the influence of boar producers from the companies PIC, Genesus Genetics and Hypor on the reproductive qualities of sows of a large white breed from the company Hypor. To implement the experiment, according to the principle of pairs of analogues, 3 groups of sows of a large white breed from the company Hypor were formed, 5 heads each. The sows of group 1 were inseminated with the seed of Landrace boars from PIC, group 2 with the seed of Landrace boars from Genesus Genetics, group 3 with the seed of Landrace boars from Hypor.Results. In scientific and economic experience, it was found that the multiplicity of sows of the 3rd group amounted to 13.0 heads, which is 7.7% less than in the 1st and 2nd experimental groups. The number of piglets at the time of weaning in groups 1 and 2 per sow was 12, which is 5.3% more than in group 3. For 1 kg of live weight gain, 9.38 EQ was spent on farrowing in the 3rd group, which is 8.1% more than in the 1st and 8.4% more than in the 2nd experimental group. Based on 1000 rubles of feed consumed, in addition, compared with the 3rd group (5.44 rubles), more live weight gain was obtained in the 1st experimental (by 0.44 rubles) and in the 2nd experimental (by 0.47 rubles) groups.
Design an Integrated Web-Based Plant Commodity Monitoring Information System
Authors: Yulianto Achmad , Widya Moh Anshori Aris 
Published: August 2024

In order to increase agricultural commodities in the Jombang Regency area to be higher, innovations are needed that can have a positive impact on the progress of the existing agricultural sector. One aspect that can support this progress is the existence of a plant commodity monitoring information system. The crop commodity monitoring system is a web-based application aimed at viewing and assessing the process of food crop growth systematically, starting from the planting period to the harvest period. From this system , historical crop data will be obtained, where these data can then be used as knowledge material for the Jombang Regency Agriculture Office in terms of policy making, such as the supply of seeds and fertilizer needs on subsidized and non-subsidized land, determination of planting periods, or predictions of agricultural products next year. In addition, this system also provides information related to plant conditions, plant age and instructions in determining plants to suit existing land. To realize this, the author designed and built an integrated web-based crop commodity monitoring information system on agricultural land. This research uses R&D (Research and Development) method. The test results using Black Box show that the functionality of this information system has been running well. The final result obtained from this study is that the system is more efficient and has comprehensive data than the old system that has been operating in the Jombang Regency Agriculture Office.
Keywords: Agriculture, Plant Commodities, Monitoring, Web, Jombang Regency Agriculture Office
Ameloblastoma radiographic imaging on 3D CBCT: a literature review
Authors: Uke Waode Anita Wulanduri , Yunus Barunawaty 
Published: August 2024

Objectives: This review article is aimed to provide an overview of 3D CBCT in determining the diagnosis of ameloblastoma.
Review: This study is a literature review consisting of English articles about the ameloblastoma radiographic imaging on 3D CBCT, published 2013-2023.The article search databases used were Google scholar, Ebsco, Pubmed .The total search results for articles based on keywords obtained were 552 articles, and only 9 articles were include. Ameloblastoma is a persistent and locally invasive tumor; with aggressive but docile growth characteristics. Ameloblastoma is generally associated with impacted teeth, so it requires a more detailed radiographic examination. Computed cone-beam tomography (CBCT) is a more advantageous imaging system with a lower radiation dose and smaller area requirements. 3D CBCT is a radiographic examination with a high modality, so it is very important in helping to establish a diagnosis, especially for cases that show radiographic differences. Ameloblastoma is divided into several types based on the radiological picture. Odontogenic Keratosis Cysts and ameloblastoma may exhibit similar radiographic features, which make diagnosis difficult.
Conclusion: 3D CBCT examination is helpful in diagnosing and validating the treatment of ameloblastoma.
HUBUNGAN PENGETAHUAN DAN SIKAP IBU HAMIL TENTANG COVID-19 DENGAN PERILAKU VAKSINASI DI WILAYAH KERJA PUSKESMAS TELAGA DEWA KOTA BENGKULU 2021
Authors: Nadhyifa  , Eka Nugraheni Diah , Yuniarti  
Published: August 2024

Abstrak : Menurut World Health Organization(WHO) Sejak akhir Desember 2019, di Wuhan, Republik Rakyat Tiongkok, munculnya wabah yang disebabkan oleh salah satu jenis betacoronavirus. Pemerintah telah melakukan berbagai upaya yang di lakukan untuk mencegah covid-19 khususnya pada ibu hamil. Salah satunya dengan vaksinasi. untukmengetahui hubungan tentang pengetahuan dan sikapibu hamil trimester III terhadap perilaku vaksinasicovid-19 di puskesmas Telaga Dewa Kota BengkuluTahun 2021.
Jenis Penelitian ini adalah pendekatan cross sectional.Populasi pada penelitian ini merupakan Ibu Hamiltrimester III di wilayah kerja Puskesmas Telaga Dewa. Jumlah populasi pada ibu hamil di Wilayah KerjaPuskesmas Telaga Dewa adalah sebesar 411 ibu hamiltrimester III, dengan pengambilan sampelmenggunakan rumus Lemeshow sehingga di dapatkan78 responden yang akan di teliti. Instrumen penelitianmenggunakan kuesioner. Analisis data menggunakanchi square.
Hasil uji chi square menunjukkan nilai 0,033 (<0,05) artinya Ada hubungan yang signifikan antarapengetahuan dengan perilaku vaksinasi ibu hamil. Hasil analisis uji chi square di dapatkan nilai 0,010 (<0,05). Dari hasil di atas di ketahui bahwa adahubungan yang signifikan antara sikap denganperilaku vaksinasi ibu hamil.
Diharapkan agar dapat mengupayakan peningkatanpengetahuan tentang covid-19 dan vaksinasi baikmelalui internet maupun mengikuti pendidikankesehatan sehingga dapat melakukan vaksinasi gunauntuk mengurangi resiko terinfeksi covid-19.
Kata kunci : Covid-19, Vaksinasi ibu hamil, Pengetahuan, Sikap
Optimasi Pemanfaatan Penggunaan ChatGPT Dalam Mengolah Bahan Ajar Untuk Guru-Guru SD
Authors: Christy Tika , Efendi Zulfan , Saputra Roni , Rahim Radiyan , Wendra Yumai 
Published: August 2024

The sophistication of technology is none other than supported by the development of AI. AI, or Artificial Intelligence, is a technology designed to create a system that mimics human capabilities. The capabilities of AI nowadays are beyond doubt as AI can analyze data quickly, make decisions very accurately, and help process existing data in a short period. ChatGPT is a chatbot developed by OpenAI, designed to understand and respond to natural language input. The utilization of AI-based chat systems like ChatGPT in the context of technology-based education 4.0 provides benefits for both students and educators. The purpose of this community service activity is to enhance the knowledge and creativity of elementary school teachers in the city of Padang. Keywords: artificial intelligence; teaching materials; ChatGPT Abstrak: Kecanggihan teknologi tidak lain didukung oleh perkembangan AI. AI atau Artificial Intelligence merupakan teknologi yang dirancang untuk membuat suatu sistem yang meniru kemampuan dari manusia. kemampuan AI untuk saat sekarang ini sudah tidak diragukan lagi dikarenakan AI dapat menganalisa data dengan cepat, bisa membuat suatu keputusan dengan sangat akurat serta dapat membantu dalam hal mengolah data yang sudah ada dalam jangka waktu yang singkat. ChatGPT merupakan chatbot yang dikembangkan oleh perusahaan OpenAI yang dirancang untuk memahami dan merespon masukan bahasa alami. Pemanfaatan sistem obrolan berbasis AI seperti ChatGPT dalam konteks pendidikan berbasis teknologi 4.0 menyediakan manfaat bagi peserta didik maupun pendidik. Dengan adanya kegiatan pengabdian kepada masyarakat ini dilaksanakan adalah untuk menambah pengetahuan dan kreatifitas guru-guru tingkat SD di kota padang. Kata kunci: bahan ajar; ChatGPT; kecerdasan buatan
Approaches to conducting a physiologically relevant test (PRT) in the study of medicines containing substance IIc of the BCS subclass using sorafenib as an example
Authors: Suvorova A. V. , Medvedev Yu. V. , Losenkova P. A. , Kramarenko O. S. , Malashenko E. A. , Poluyanov A. M. , Shohin I. E. 
Published: August 2024

Introduction. Sorafenib is an antineoplastic drug belonging to class IIc according to the biopharmaceutical classification system (BCS) due to the presence of both acidic and basic properties. In addition to low solubility, sorafenib is characterized by high variability during clinical trials, in particular bioequivalence studies (BE). To selecting batches that can be recommended for BE studies, the dissolution kinetics test is currently widely used, however, the results of this test are not always sufficient and additional tests, for example, a physiologically relevant test, are advisable. To minimize the risks of obtaining nonequivalent results during the BE study, a physiologically relevant test (PRT) was carried out with further data processing and interpretation of the results of physiologically based pharmacokinetic modeling (PBPK).Aim. The aim of the study is to conduct a physiologically relevant test (PRT) for the purpose of selecting a candidate batch for subsequent BE study of sorafenib drugs using the physiologically based pharmacokinetic model (PBPK).Materials and methods. The objects of the study are Nexavar®, film-coated tablets, 200 mg (Bayer AG, Germany) (one batch) and Sorafenib, film-coated tablets, 200 mg (two batches) (Russia). The physiologically relevant test was performed on the SC PRT-6 device (LLC "Scientific Compliance", Russia). Quantitative analysis was performed by HPLC-UV on the Chromatec-Crystal HPLC 2014 device (CJSC "Chromatec", Russia). The plasma concentration profiles were simulated using PK-Sim® software (Systems Biology Software Suite 11.2, Bayer Technology Services GmbH, Germany).Results and discussion. As part of the study, a method for the quantitative determination of sorafenib was developed and validated, a method for sample preparation was developed, and a method for conducting the PRT for sorafenib, as a representative of the IIc subclass of BCS, was developed. Based on the study results, release profiles were obtained that were used to select a candidate series for the BE study. The series were selected based on the PBPK analysis on a virtual population consisting of 36 healthy volunteers with activated enteropathic circulation, characteristic of sorafenib.Conclusion. The PRT was carried out for the drug sorafenib. Quantitative determination was carried out by HPLC-UV according to the developed and validated method. The test resulted in obtaining data that were subjected to PBPK analysis. It was shown that the studied batches have high risks of non-equivalence during the bioequivalence study.
CONSTELLATION AND VERNACULARIZATION OF JAVANESE TEXTURE OF THE QUR’AN: CHARACTERISTICS AND CULTURAL LOCALITY OF JAVANESE PONDOK PESANTREN IN TAFSIR AL-IBRĪZ LI MA’RIFAH TAFSĪR AL-QUR’AN AL-AZĪZ
Authors: Dewi Ajeng Indira 
Published: August 2024

The existence of the Qur’an as a holy book that is salih li kulli zaman wa makan is relevant to the complexity of traditions and societal problems that are experiencing development and change, which more or less influence the diversity of understanding of the Qur’an. Like the spread of Islam to Indonesia, it has implications for the emergence of many products of Qur’an interpretation, and a product of interpretation cannot be separated from space and time. Like the book of Tafsir Al-Ibriz, it is a form of response and reception from Bisri Musthafa for reading the text of the Qur’an, a dialectic between text, context, and interpreter. The book of Tafsir Al-Ibriz has a strong locality. Supported by Bisri Musthafa’s excellence as the book's author, he also has his own added values, such as the ability to articulate, documentation, organizing which have implications for his product interpretations. This research is a type of library research supported by descriptive analytic interpretation. Based on the study results, it was concluded that the contents of the book Tafsir Al-Ibriz were written over four years using a textual-contextual approach (more dominant textual). This interpretation has several features, including fiqhi, sufi, and adabi ijtima’i. His interpretation is coloured by the life in which he lives, namely the pesantren environment, as well as his scientific journey for two years in Mecca, as well as a socio-religious organization in which he is active as one of the administrators and initiators of his qanun asasi, namely Nahdhatul Ulama.