bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolic engineering of Escherichia coli for the production of isoflavonoid‐4′‐O‐methoxides and their biological activities
Authors: Koirala Niranjan , Pandey Ramesh Prasad , Thuan Nguyen Huy , Ghimire Gopal Prasad , Jung Hye Jin , Oh Tae‐Jin , Sohng Jae Kyung 
Published: October 2015

AbstractIsoflavonoid representatives such as genistein and daidzein are highly potent anticancer, antibacterial, and antioxidant agents. It have been demonstrated that methylation of flavonoids enhanced the transporting ability, which lead to facilitated absorption and greatly increased bioavailability. In this paper, genetically engineered Escherichia coli was reconstructed by harboring E. coli K12‐derived metK encoding S‐adenosine‐l‐methionine (SAM) synthase (accession number: K02129) for enhancement of SAM as a precursor and Streptomyces avermitilis originated SaOMT2 (O‐methyltransferase, accession number: NP_823558) for methylation of daidzein and genistein as preferred substrates. The formation of desired products via biotransformation including 4′‐O‐methyl‐genistein and 4′‐O‐methyl‐daidzein was confirmed individually by using chromatographical methods such as high‐performance liquid chromatography, liquid chromatography/time‐of‐flight/mass spectrometry (LC‐TOF‐MS), and nuclear magnetic resonance (NMR), and NMR (1H and 13C). Furthermore, substrates concentration, incubation time, and media parameters were optimized using flask culture. Finally, the most fit conditions were applied for fed‐batch fermentation with scale‐up to 3 L (working volume) to obtain the maximum yield of the products including 164.25 µM (46.81 mg/L) and 382.50 µM (102.88 mg/L) for 4′‐O‐methyl genistein and 4′‐O‐methyl daidzein, respectively. In particular, potent inhibitory activities of those isoflavonoid methoxides against the growth of cancer line (B16F10, AGS, and HepG2) and human umbilical vein endothelial cells were investigated and demonstrated. Taken together, this research work described the production of isoflavonoid‐4′‐O‐methoxides by E. coli engineering, improvement of production, characterization of produced compounds, and preliminary in vitro biological activities of the flavonoids being manufactured.
A selective chromofluorogenic chemodosimeter for fluoride ions based on distyrylbenzenes derivatives containing dual Si–O groups
Authors: Ji Kai , Wu Haocheng , Chen Jinju , Shen Jiajie , Wang Xinyu , Wu Hongwei , Liu Chuanxiang 
Published: October 2015

AbstractA new chemodosimeter for fluoride ions based on F− triggered dual Si–O bond cleavage of distyrylbenzenes derivatives was developed for the first time. Upon the addition of F− ions, the chemodosimeter (7) displayed apparent color changes from colorless to faint yellow, with a dramatic red‐shift in the emission wavelength (~100 nm), and higher selectivity for fluoride over other common anions. With the aid of fluorescence measurements, the limit of detection could be as low as 89.8 nm. Using a ‘dipstick’ approach, chemodosimeter (7) might serve as a prototype device for fluorescent detecting F− without the need for any additional equipment. Copyright © 2015 John Wiley & Sons, Ltd.
Cell differentiation on disk‐ and string‐shaped hydrogels fabricated from Ca2+‐responsive self‐assembling peptides
Authors: Fukunaga Kazuto , Tsutsumi Hiroshi , Mihara Hisakazu 
Published: October 2015

ABSTRACTWe recently developed a self‐assembling peptide, E1Y9, that self‐assembles into nanofibers and forms a hydrogel in the presence of Ca2+. E1Y9 derivatives conjugated with functional peptide sequences derived from extracellular matrices (ECMs) reportedly self‐assemble into peptide nanofibers that enhance cell adhesion and differentiation. In this study, E1Y9/E1Y9‐IKVAV‐mixed hydrogels were constructed to serve as artificial ECMs that promote cell differentiation. E1Y9 and E1Y9‐IKVAV co‐assembled into networked nanofibers, and hydrogels with disk and string shapes were formed in response to Ca2+ treatment. The neuronal differentiation of PC12 cells was facilitated on hydrogels of both shapes that contained the IKVAV motifs. Moreover, long neurites extended along the long axis of the string‐shaped gel, suggesting that the structure of hydrogels of this shape can affect cellular orientation. Thus, E1Y9 hydrogels can potentially be used as artificial ECMs with desirable bioactivities and shapes that could be useful in tissue engineering applications. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 106: 476–483, 2016.
Inquiry‐based experiments for large‐scale introduction to PCR and restriction enzyme digests
Authors: Johanson Kelly E. , Watt Terry J. 
Published: October 2015

AbstractPolymerase chain reaction and restriction endonuclease digest are important techniques that should be included in all Biochemistry and Molecular Biology laboratory curriculums. These techniques are frequently taught at an advanced level, requiring many hours of student and faculty time. Here we present two inquiry‐based experiments that are designed for introductory laboratory courses and combine both techniques. In both approaches, students must determine the identity of an unknown DNA sequence, either a gene sequence or a primer sequence, based on a combination of PCR product size and restriction digest pattern. The experimental design is flexible, and can be adapted based on available instructor preparation time and resources, and both approaches can accommodate large numbers of students. We implemented these experiments in our courses with a combined total of 584 students and have an 85% success rate. Overall, students demonstrated an increase in their understanding of the experimental topics, ability to interpret the resulting data, and proficiency in general laboratory skills. © 2015 by The International Union of Biochemistry and Molecular Biology, 43:441–448, 2015.
Induction of neural differentiation in rat C6 glioma cells with taxol
Authors: Chao Chuan‐Chuan , Kan Daphne , Lo Ta‐Hsuan , Lu Kuo‐Shyan , Chien Chung‐Liang 
Published: October 2015

AbstractBackgroundGlioblastoma is a common and aggressive type of primary brain tumor. Several anticancer drugs affect GBM (glioblastoma multiforme) cells on cell growth and morphology. Taxol is one of the widely used antineoplastic drugs against many types of solid tumors, such as breast, ovarian, and prostate cancers. However, the effect of taxol on GBM cells remains unclear and requires further investigation.MethodsSurvival rate of C6 glioma cells under different taxol concentrations was quantified. To clarify the differentiation patterns of rat C6 glioma cells under taxol challenge, survived glioma cells were characterized by immunocytochemical, molecular biological, and cell biological approaches.ResultsAfter taxol treatment, not only cell death but also morphological changes, including cell elongation, cellular processes thinning, irregular shapes, and fragmented nucleation or micronuclei, occurred in the survived C6 cells. Neural differentiation markers NFL (for neurons), βIII‐tubulin (for neurons), GFAP (for astrocytes), and CNPase (for oligodendrocytes) were detected in the taxol‐treated C6 cells. Quantitative analysis suggested a significant increase in the percentage of neural differentiated cells. The results exhibited that taxol may trigger neural differentiation in C6 glioma cells. Increased expression of neural differentiation markers in C6 cells after taxol treatment suggest that some anticancer drugs could be applied to elimination of the malignant cancer cells as well as changing proliferation and differentiation status of tumor cells.
Consumers' attributions and brand evaluations in product‐harm crises: The role of implicit theories of personality
Authors: Yin Cheng‐Yue , Yu Hong‐Yan , Poon Patrick 
Published: October 2015

AbstractThis study aims to examine the moderating role of implicit theories of personality in the relationship between corporate recovery strategy (i.e., support versus stonewalling) and consumers' attributions (and brand evaluations). It is suggested that consumers' implicit theories about the fixedness/malleability of personality can affect consumers' attributions and brand evaluations during a product‐harm crisis. In addition, corporate image (i.e., strong versus weak) can moderate the influence of the role of implicit theories of personality. Two experiments were conducted to examine the proposed hypotheses. Results of Experiment 1 show that consumers who endorse entity theory (i.e., entity theorists) are likely to attribute crisis as more internal, stable, and controllable, particularly when they do not have any prior knowledge about the firm. The entity theorists would have more negative brand evaluations than incremental theorists (who endorse incremental theory), when “support” strategy was used by the firm. Results of Experiment 2 show that entity theorists are prone to have more external (internal) and unstable (stable) attributions toward a firm with a strong (weak) corporate image. Furthermore, entity theorists would provide more positive brand evaluations than incremental theorists when “stonewalling” strategy was used by a firm with strong corporate image, but not when “support” strategy was used by a firm with weak corporate image. Managerial implications are provided to managers with regard to product‐harm crisis and recovery strategies. Copyright © 2015 John Wiley & Sons, Ltd.
Aortic root volume is associated with contained rupture of the aortic annulus in balloon‐expandable transcatheter aortic valve replacement
Authors: Reinöhl Jochen , Psyrakis Dimitrios , Kaier Klaus , Kodirov Sodikdjon , Siepe Matthias , Gutmann Anja , von zur Mühlen Constantin , Moser Martin , Ahrens Ingo , Pache Gregor , Zirlik Andreas , Langer Mathias , Beyersdorf Friedhelm , Zehender Manfred , Bode Christoph , Blanke Philipp 
Published: October 2015

BackgroundAortic annulus rupture is a rare, but potentially fatal complication of transcatheter aortic valve replacement (TAVR), especially when it occurs by balloon‐expandable devices. In order to improve the predictability of procedures and avoid ruptures we investigated whether or not the aortic root volume measures is a useful indicator of risk, and if it could be useful for the prosthesis size selection.Methods and ResultsFrom a retrospective series of 172 TAVR patients, seven experienced contained aortic annulus ruptures. The receiver operating curves were used to illustrate sensitivity and specificity of the different aortic annulus size and aortic root volume measures. The annulus area oversizing of ≥20% resulted in a sensitivity of 100%, specificity of 55.76%, and positive predictive value (PPV) of 8.75%. In patients receiving 26mm prostheses, the aortic root volume (ARV <13600mm3) provided a better specificity and PPV (79.63 and 18.52%, respectively). A two‐step testing procedure considering the area derived average annulus diameter (Darea <23mm) as a first separating parameter and then the ARV (<13,600mm3) as a further indicator showed the most promising results with the PPV of 31.25%. Regardless of the procedure steps no false negative results were predicted.ConclusionsOur data show that the ARV provides a better predictive value for correct prosthesis sizing than established annulus measurements, especially in ‘borderline’ annuli. We suggest a two‐step testing procedure for prostheses size selection, considering Darea and ARV to minimize the risk of annulus rupture. Prospective studies and examination of larger datasets are warranted to confirm these findings. © 2015 Wiley Periodicals, Inc.
Controlled Nanopores in Thin Films of Nonstoichiometrically Supramolecularly Assembled Graft Copolymers
Authors: Cho Suk Man , Song Giyoung , Hwang Sun Kak , Kim Richard Hahnkee , Lee Jinseong , Yu Seunggun , Huh June , Park Hui Joon , Park Cheolmin 
Published: October 2015

AbstractHerein, nanometer‐scale morphologies of graft‐copolymer‐like supramolecular thin films, composed of sulfonic acid terminated polystyrene (SPS) and poly(2‐vinylpyridine) (P2VP), and their application to antireflection coatings were investigated. The intermolecular complexes of SPS and P2VP, formed through nonstoichiometric multiple hydrogen bonding between the sulfonic acid group of SPS and the nitrogen atom in pyridine unit of P2VP, occurring in film deposition allowed for the formation of spherical micelles (with SPS and P2VP as the corona and core, respectively) in the thin film. Interestingly, the domain size of the micelles was tunable from approximately 20 to 90 nm on average by controlling either the blend ratio of components or the concentration of polymer solution. Furthermore, nanoporous thin films could be easily prepared by removing the core of micelle‐based nanostructures by using a simple solvent etching process, leaving sulfonic acid groups on the surface of nanopores, which can be utilized as potential functional sites. Those resultant nanoporous thin films were conveniently employed as an antireflection layer on a glass substrate, giving a maximum 97.8 % transmittance in the visible wavelength range.
Controlled Redox Chemistry at Cerium within a Tripodal Nitroxide Ligand Framework
Authors: Bogart Justin A. , Lippincott Connor A. , Carroll Patrick J. , Booth Corwin H. , Schelter Eric J. 
Published: October 2015

AbstractLigand reorganization has been shown to have a profound effect on the outcome of cerium redox chemistry. Through the use of a tethered, tripodal, trianionic nitroxide ligand, [((2‐tBuNOH)C6H4CH2)3N]3− (TriNOx3−), controlled redox chemistry at cerium was accomplished, and typically reactive complexes of tetravalent cerium were isolated. These included rare cationic complexes [Ce(TriNOx)thf][BArF4], in which ArF=3,5‐(CF3)2‐C6H3, and [Ce(TriNOx)py][OTf]. A rare complete Ce–halide series, Ce(TriNOx)X, in which X=F−, Cl−, Br−, I−, was also synthesized. The solution chemistry of these complexes was explored through detailed solution‐phase electrochemistry and 1H NMR experiments and showed a unique shift in the ratio of species with inner‐ and outer‐sphere anions with size of the anionic X− group. DFT calculations on the series of calculations corroborated the experimental findings.
Efficient Domino Hydroformylation/Benzoin Condensation: Highly Selective Synthesis of α‐Hydroxy Ketones
Authors: Dong Kaiwu , Sang Rui , Soule Jean‐Francois , Bruneau Christian , Franke Robert , Jackstell Ralf , Beller Matthias 
Published: October 2015

AbstractAn improved domino hydroformylation/benzoin condensation to give α‐hydroxy ketones has been developed. Easily available olefins are smoothly converted into the corresponding α‐hydroxy ketones in high yields with excellent regioselectivities. Key to success is the use of a specific catalytic system consisting of a rhodium/phosphine complex and the CO2 adduct of an N‐heterocyclic carbene.
Enantioselective NHC‐Catalyzed Redox [2+2] Cycloadditions with Perfluoroketones: A Route to Fluorinated Oxetanes
Authors: Davies Alyn T. , Slawin Alexandra M. Z. , Smith Andrew D. 
Published: October 2015

AbstractThe N‐heterocyclic carbene (NHC) catalyzed redox formal [2+2] cycloaddition between α‐aroyloxyaldehydes and perfluoroketones, followed by ring‐opening in situ delivers a variety of perfluorinated β‐hydroxycarbonyl compounds in good yield, and excellent diastereo‐ and enantioselectivity. Through a reductive work‐up and subsequent cyclization, this protocol offers access to highly substituted fluorinated oxetanes in two steps and in high ee.
Informed consent conversations and documents: A quantitative comparison
Authors: Koyfman Shlomo A. , Reddy Chandana A. , Hizlan Sabahat , Leek Angela C. , Kodish and Eric D. ,   on behalf of the Phase I Informed Consent (POIC) Research Team
Published: October 2015

BACKGROUNDInformed consent for clinical research includes 2 components: informed consent documents (ICDs) and informed consent conversations (ICCs). Readability software has been used to help simplify the language of the ICD, but to the authors' knowledge is rarely used to assess the language used during the ICC, which may influence the quality of informed consent. The current analysis was performed to determine whether length and reading levels of transcribed ICCs are lower than their corresponding ICDs for selected clinical trials, and to assess whether investigator experience affected the use of simpler language and comprehensiveness.METHODSThe current study was a prospective study in which ICCs were audiorecorded at 6 institutions when families were offered participation in pediatric phase I oncology trials. Word count, Flesch‐Kincaid Grade Level (FKGL), and Flesch Reading Ease score (FRES) of the ICCs were compared with corresponding ICDs, including the frequency with which investigators addressed 8 prespecified critical consent elements during the ICC.RESULTSSixty‐nine unique physician/protocol pairs were identified. Overall, ICCs contained fewer words (4677 vs 6364 words; P = .0016) and had a lower FKGL (6 vs 9.7; P ≤ .0001) and a higher FRES (77.8 vs 56.7; P<.0001) compared with their respective ICDs, but were more likely to omit critical consent elements, such as voluntariness (55%) and dose‐limiting toxicities (26%). Years of investigator experience was not correlated with reliably covering critical elements or decreased linguistic complexity.CONCLUSIONSClinicians use more understandable language during ICCs than the corresponding ICD, but appear to less reliably cover elements critical to fully informed consent. Efforts focused at providing communication training for clinician‐investigators should be made to optimize the synergy between the ICD and the ICC. Cancer 2016;122:464–469. © 2015 American Cancer Society.
Cross‐Polarization Electron‐Nuclear Double Resonance Spectroscopy
Authors: Rizzato Roberto , Bennati Marina 
Published: October 2015

AbstractMagnetic nuclei in the proximity of a paramagnetic center can be polarized through electron‐nuclear cross‐polarization and detected in electron‐nuclear double resonance (ENDOR) spectroscopy. This principle is demonstrated in a single‐crystal model sample as well as on a protein, the β2 subunit of E.coli ribonucleotide reductase (RNR), which contains an essential tyrosyl radical. ENDOR is a fundamental technique to detect magnetic nuclei coupled to paramagnetic centers. It is widely employed in biological and materials sciences. Despite its utility, its sensitivity in real samples is about one to two orders of magnitude lower than conventional electron paramagnetic resonance, thus restricting its application potential. Herein, we report the performance of a recently introduced concept to polarize nuclear spins and detect their ENDOR spectrum, which is based on electron‐nuclear cross polarization (eNCP). A single‐crystal study permits us to disentangle eNCP conditions and CP‐ENDOR intensities, providing the experimental foundation in agreement with the theoretical prediction. The CP‐ENDOR performance on a real protein sample is best demonstrated with the spectra of the essential tyrosyl radical in the β2 subunit of E.coli RNR.
Monoanionic Dipyrrin–Pyridine Ligands: Synthesis, Structure and Photophysical Properties
Authors: Ducloiset Clémence , Jouin Pauline , Paredes Elisa , Guillot Régis , Sircoglou Marie , Orio Maylis , Leibl Winfried , Aukauloo Ally 
Published: October 2015

AbstractA novel monoanionic tetradentate N4 ligand (F5DPPy) based on a dipyrromethene skeleton as a molecular platform and decorated with pyridine rings at the 1‐ and 9‐positions of the dipyrrin motif has been prepared and characterized. Interestingly, although this ligand is weakly fluorescent, it presents a chelation‐enhanced fluorescence effect of around 150 times upon coordination to ZnII. Time‐dependent (TD) DFT calculations reproduce nicely the spectroscopic features of both the ligand and the complex, and analysis of the electron density redistribution in the excited state suggests that a better orbital overlap of the HOMO and LUMO in F5DPPyZnCl compared with F5DPPy is responsible for the more intense transitions observed with the former system. As such, this ligand opens interesting perspectives in the design of ratiometric sensors.
Bright Cyan Phosphorescence of a (Phosphane)copper(I) Complex of the Tri­hydrido­pyrazolylborate Ligand H3B(3,5‐Ph2Pz)–
Authors: van Dijkman Thomas F. , de Bruijn Hans M. , Siegler Maxime A. , Bouwman Elisabeth 
Published: October 2015

AbstractThe ligand trihydrido(3,5‐diphenylpyrazol‐1‐yl)borate([MpPh2]–) has been synthesized from 3,5‐diphenylpyrazole and sodium borohydride in N,N‐dimethylformamide. By using this ligand, three phosphane‐stabilized copper(I) complexes [Cu(MpPh2)(L2)] [L2 = (PPh3)2, 1,2‐bis(diphenylphosphanyl)ethane (dppe), or 1,2‐bis(diphenylphosphanyl)benzene (dppbz)] were synthesized. The complexes were characterized by multinuclear NMR spectroscopy, IR spectroscopy, and X‐ray crystallography. The crystal structures of these complexes show that the copper ions are in trigonal‐pyramidal geometries with the apical position formed by agostic Cu–H interactions between the CuI center and one of the hydride atoms of the borate ligand. Complexes [Cu(MpPh2)(PPh3)2] and [Cu(MpPh2)(dppbz)] are mononuclear, whereas complex [Cu2(MpPh2)2(μ‐dppe)2] is dinuclear with bridging dppe ligands. In the solid state, fluorescent emissions are observed in [Cu(MpPh2)(PPh3)2] and [Cu2(MpPh2)2(μ‐dppe)2] but not in [Cu(MpPh2)(dppbz)], which exhibits bright cyan phosphorescence at room temperature that shifts to green when the sample is cooled to 77 K. The phosphorescence of [Cu(MpPh2)(dppbz)] is attributed to mixed interligand and metal‐to‐ligand charge transfer 3(MLCT + π–π*) excited states.
A data fusion approach for spatial analysis of speciated PM2.5 across time
Authors: Rundel Colin W. , Schliep Erin M. , Gelfand Alan E. , Holland David M. 
Published: October 2015

PM2.5 exposure is linked to a number of adverse health effects such as lung cancer and cardiovascular disease. However, PM2.5 is a complex mixture of different species whose composition varies substantially in both space and time. An open question is how these constituent species contribute to the overall negative health outcomes seen from PM2.5 exposure. To this end, the Environmental Protection Agency as well as other federal, state, and local organization monitor total PM2.5 along with its primary species on a national scale. From an epidemiological perspective, there is a need to develop effective methods that will allow for the spatially and temporally sparse observations to be used to predict exposures for locations across the entire United States.Toward this objective, we have collected data from three separate monitoring station networks as well as output from a deterministic atmospheric computer model. We introduce a novel multi‐level speciated PM2.5 model, which captures the following features: (1) it fuses data from three monitoring networks; (2) it simultaneously models each of the five primary components of PM2.5 from each network along with the computer model output; (3) it introduces species and network level measurement error models as well as total PM2.5 measurement error models, all varying around the respective latent true levels; (4) it incorporates an unobserved “other” species component as well as a sum constraint such that the total is physically consistent (i.e., total must be equal to the sum of the primary species and “other”), which is not always the case with the observed data. Copyright © 2015 John Wiley & Sons, Ltd.