bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE ADHESION OF STAPHYLOCOCCUS EPIDERMIDIS TO CULTURED CELLS
Authors: Kawashima Takeshi 
Published: April 2015

ABSTRACTIn this study, the author observed the adhesion of Staphylococcus (St.) epidermidis to three kinds of cultured cells—human epidermal cells (JTC‐17), human synovial cells (McCoy) and mouse connective tissue cells (L). In the same manner, St. aureus, Micrococcus (M.) luteus and Pseudomonus (Ps.) aeruginosa adhered to the cells, but Escherichia (E.) coli did not. Heat killing M. luteus decreased its adhesiveness, and heat killing Ps. aeruginosa eliminated its ability, but the adhesion of Staphylococci was not altered by the heat treatment. The adhesiveness of living bacteria was also evaluated by a colony forming unit (CFU) counting study. St. epidermidis adhered to the same extent as St. aureus, which is reported to adhere to epithelial cells through fibronectin. Human plasma fibronectin (Fn) treatment inhibited the adhesiveness of St. epidermidis to JTC‐17 cells. This suggests that St. epidermidis may have a receptor for Fn as St. aureus does.
ANTI‐HAIR KERATIN MONOCLONAL ANTIBODY (HKN‐2)
Authors: Tazawa Toshio , Ito Masaaki , Ito Kaoru , Shimizu Naoya , Sato Yoshio 
Published: April 2015

ABSTRACTBALB/c mice were immunized with human hair fibrous proteins. Then their spleen cells were fused with Sp2/0‐Agl4 mouse myeloma cells. Antibody‐producing hybridomas were selected by ELISA method and cloned by limiting dilution. A monoclonal antibody was chosen and designated as HKN‐2. Immunohistochemically, on frozen sections of normal human skin, HKN‐2 was found to recognize the suprabasal cells of epidermis and hair follicle, the cells in the keratogenous zone of hair shaft, the sebaceous cells and the ductal and myoepithelial cells of sweat glands. The basal cells of the epidermis and lower hair follicle, hair matrix cells and secretory cells of sweat glands did not react with HKN‐2. An immunoelectron microscopic method showed that the positive reaction to HKN‐2 was located on the tonofilaments in the cytoplasm. It was concluded that there might be a common antigenic determinant between hair and other skin epithelial tissues. A complexity in keratin manifestation in each epithelial tissue of the skin was suspected.
RECURRENT ECZEMA HERPETICUM IN AN ADULT
Authors: Hata Seiichiro 
Published: April 2015

ABSTRACTA case of recurrent Kaposi's varicelliform eruption caused by herpes simplex virus type 1 was presented in a man with atopic dermatitis. The generalized vesicular eruptions first developed during his twenty sixth year. Four years later the same vesicular lesion as wide as the first attack developed and was improved in a shorter period.
SEASONAL PREVALENCE OF ERYTHEMA INFECTIOSUM
Authors: Saburi Yasuo , Takahashi Toyozoh , Okuda Kenji 
Published: April 2015

ABSTRACTDuring the five years from 1979 to 1983, seasonal variations in epidemics of erythema infectiosum was studied. The peak incidence appeared in April in 1981 and 1983, in May in 1979, and in June in 1980 and 1982. These results suggest that the disease is a springtime one.
LICHEN SCLEROSUS ET ATROPHICUS IN CHILDREN
Authors: Muramatsu Tsutomu , Kitamura Wataru , Sakamoto Kuniki 
Published: April 2015

ABSTRACTA 7‐year‐old girl with lichen sclerosus et atrophiais on her back was reported. The lesion showed a complete clinical cure after one year of topical corticosteroid therapy without any significant atrophy. The prognosis of lichen sclerosus et atrophicus in children is better than that in adult cases, so spontaneous resolution could not be ruled out.
CLINICAL CHARACTERISTICS OF SUBUNGUAL MELANOMAS IN JAPAN
Authors: Miura Shunsuke , Jimbow Kowichi 
Published: April 2015

ABSTRACTThis study reported 2 cases of subungual melanomas and analyzed, based on a questionnaire survey of 108 cases, clinical characteristics of Japanese subungual melanomas in relation to trauma history. We found (a) that subungual melanomas comprice about 10% of all cutaneous melanomas in Japan, (b) that 75% of them occur on fingers, particularly on the first finger, (c) that there is not a significant relationship between trauma history and occurrence or anatomic site of subungual melanomas, except for those of the right first finger where young males predominate and (d) that no differences exist in the histopathological types between the groups with and without trauma history. The relatively high incidence of subungual melanomas in Japanese appears to be related to genetic background and not directly to frequent exposure to trauma.
INFLUENCE OF ROUTE ON THE INDUCTION AND PERSISTENCE OF DELAYED TYPE HYPERSENSITIVITY TO ALLOANTIGENS
Authors: Tamaki Kunihiko , Furue Masutaka 
Published: April 2015

ABSTRACTThe influence of the route on the induction and persistence of delayed type hypersensitivity (DTH) reaction to alloantigens by epidermal cells (EC) and spleen cells (SC) was investigated. Subcutaneous (sc) or intraperitoneal (ip) administration of EC and SC induced DTH reactions but the DTH reaction by the ip route was weaker than that obtained by the sc route. Time course experiments have shown that DTH reactions induced by EC lasted at least 30 days, whereas DTH induced by ip injection of SC fell to an undiscernible level by day 14. DTH induced by sc injection of SC was significant at day 14, but became undiscernible by day 30. Intravenous (iv) injection of both EC and SC induced hyporesponsiveness after subsequent sensitization with allogeneic cells.Experiments using congenic strains of mice, EC treated with anti‐Ia antiserum and complement, and grafted skin revealed that H‐2, non‐H‐2, or Ia disparities were sufficient to induce DTH. Epidermal LC played an important role in DTH induction.Taken together, these results indicate the importance of LC in DTH induction and suggest the differing contributions of antigen presenting cells in the skin and other tissues in both induction and persistence of allo‐DTH reaction.
BULLOUS DERMATOSIS OF HEMODIALYSIS
Authors: Matarredona Jaime , Martín Roberto , De Salamanca Rafael Enriquez , Sendagorta Enrique , Matesanz Rafael , Perpiña Julia , Ortuño Joaquín , Ledo Antonio 
Published: April 2015

ABSTRACTBullous dermatosis developed in three patients with renal failure who were on maintenance hemodialysis. On clinical and histological grounds, skin lesions were indistinguishable from those of porphyria cutanea tarda. A highly sensitive fluorimetric thin‐layer chromatographic technique was used for assay of porphyrin levels in plasma, urine, feces and dialysate. The porphyrin metabolism alterations observed in our three patients were not sufficiently uniform to be considered the cause of the bullous dermatosis. Uroporphyrinogen decarboxylase activities in red cells were also within normal limits. No other common etiological factors could be identified in our patients.
GALACTOSE‐REACTING AGGLUTININS TO SPOROTHRIX SCHENCKII
Authors: Kobayashi Hiroto , Ishizaki Hiroshi 
Published: April 2015

ABSTRACTAgglutinins to Sporothrix schenckii were produced in rabbits by immunization with Aspergillus fumigatus. Agglutination of S. schenckii cells was inhibited by D‐galactose but not by D‐mannose. Indirect immuno‐scanning electron microscopy using rabbit anti‐A. fumigatus serum and ferritin conjugated goat anti‐rabbit IgG serum showed considerable labelling of ferritin particles at the cell surface of S. schenckii; this behavior was inhibited by D‐galactose but not D‐mannose. These results suggested that galactose determinants were present at the cell surface of S. schenckii and that agglutinins to S. schenckii were divided into two groups, galactose‐reacting antibodies as well as the already recognized rhamnose‐reacting ones.
CYTOFLUOROMETRIC STUDY OF EPIDERMAL CELL PROLIFERATION INDUCED BY ORAL ADMINISTRATION OF AROMATIC RETINOID
Authors: Nomura Hirofumi , Hachisuka Hiroshi , Sasai Yoichiro 
Published: April 2015

ABSTRACTThe rate of cell proliferation in the basal cell layer after oral administration of aromatic retinoid was investigated with the cytofluorometric method. Guinea pigs were fed aromatic retinoid in daily doses of 5 mg/kg body weight for a period of 14 days. Epidermal cell suspensions were fractionated on colloidal silica polyvinylpyrrolidon to obtain a relatively pure fraction of basal cells. After being smeared on glass slides, they were stained with acridine orange. At 1 day after the administration, there was no change in the DNA distribution pattern. However, G0/G1 ratio decreased strikingly. By 3 days, there was an increase in S cells. These results suggest that epidermal cell proliferation by oral administration of retinoid is due to the increase of cycling cells.
A UNIQUE ERYTHERMALGIA IN A PATIENT WITH VON RECKLINGHAUSEN NEUROFIBROMATOSIS
Authors: Kikuchi Ichiro , Inoue Shouhei , Tada Shigeru 
Published: April 2015

ABSTRACTWe report a 58‐year‐old female patient with von Recklinghausen neurofibromatosis who developed a condition similar to that of erythermalgia in many respects, especially on her hands. The distress worsened upon exposure to cold and improved when warmed. She felt pain over the hands restricted to the time of urination when her bladder was full. It was found that her distress was felt when blood was unstably forced through partial closing of abnormal a‐v shunts into diseased blood vessels of the distal extremities and that the a‐v shunts of this patient apparently worked differently.
ULTRASTRUCTURAL LOCALIZATION OF ANIONIC SITES IN THE RAT SKIN DEMONSTRATED WITH CATIONIC PROBES
Authors: Kazama Takashi , Kihara Itaru , Oite Takashi , Shimizu Fujio , Ito Masaaki , Sato Yoshio 
Published: April 2015

ABSTRACTFor the purpose of demonstrating ultrastructurally the distribution of anionic sites in the rat skin, we applied two polyethyleneimines of different molecular weights and ruthenium red by the perfusion or immersion method. Using each cationic probe, sites were observed as electron dense particles along both the epidermal and interstitial sides of the lamina densa in the dermo‐epidermal junction and the interstitial side of the periendothelial lamina densa in the dermal capillary. The ordered distribution of the anionic sites may function to transport fluid and macromolecular substances through these particular compartments.
GLYCOSAMINOGLYCAN SYNTHESIS IN DERMATOFIBROSARCOMA PROTUBERANS
Authors: Maekawa Yoshihiro , Nogami Reiko 
Published: April 2015

ABSTRACTA sliced tumor of dermatofibrosarcoma protuberans was labelled with 3H‐glucosamine in Ham's F12 medium with 10% fetal calf serum at 37°C in 5% CO2 with 95% air for 24 hours. Electrophoresis of extracted glycosaminoglycan was undertaken on cellulose acetate strips. The radioactive content of the separated glycosaminoglycan in the tumor was counted and compared with the glycosaminoglycan of normal skin. A larger amount of hyaluronic acid and chondroitin sulfate was synthesized in dermotofibrosarcoma protuberans than in normal skin.The qualitative and quantitative changes in glycosaminoglycan in the stroma suggest that it is unlikely to metastasize to the regional lymph nodes and internal organs.
DERMATAN SULFATE IS THE MAJOR GLYCOSAMINOGLYCAN SYNTHESIZED BY CULTURED GUINEA PIG SKIN FIBROBLASTS
Authors: Nakamura Toshitaka , Shinkai Hiroshi 
Published: April 2015

ABSTRACTFibroblasts from guinea pig skin were incubated with [3H] glucosamine. The cells secreted [3H] galactosamine‐labeled compounds predominantly as dermatan sulfate, which accounted for 65–75% of the total glycosaminoglycans. The majority production of dermatan sulfate was observed regardless of the composition of the culture medium, cell growing phase or age of animal. The labeled glycosaminoglycans were identified by enzymatic (chondroitinase ABC, chondroitinase AC and hyaluronidase) or nitrous acid treatment, followed by fluorography of two‐dimensional electrophoresis on cellulose acetate membranes. The molecular weight of the newly synthesized dermatan sulfate from medium of a confluent stage was calculated as 15,000 to 17,500 from molecular sieve chromatography. The radioactive dermatan sulfate was treated with chondroitinase ABC, yielding 68.4% ΔDi‐4S, 16.2% ΔDi‐6S, 13.3% ΔDi‐OS and 2% di‐sulfated hexuronosyl‐N‐acetylhexosamine units. Chondroitin sulfate was detected on a cellulose acetate membrane as a minor component in the culture medium from the cell growing stage. It was not detected in medium from a confluent stage. Organ culture of guinea pig skin or cell culture of human skin fibroblasts incorporated [3H] glucosamine mainly into hyaluronic acid. About 90% of newly synthesized glycosaminoglycans was accounted for by hyaluronate.
EPIDEMIOLOGICAL SURVEY OF “NYLON CLOTHS FRICTION DERMATOSIS” IN JAPAN
Authors: Tanigaki Takehiko , Hata Seiichiro , Kitano Yukio , Sano Shigeharu 
Published: April 2015

ABSTRACTA survey of nylon cloths friction dermatosis (NCFD) was taken by gathering information by a questionnaire sent to university hospitals in Japan. The replies were obtained from 44 hospitals (55% recovery) reporting 158 patients. Pigmentation was noted mainly on the skin over the trunk bones and extremities. The pigmentation was distributed over more than two regions in most of the patients. Along with the clinical and histological features, a history of repeated friction, usually with nylon towels or scrub brushes, was required for diagnosis. A few months after discontinuing the use of nylon towels or scrub brushes, NCFD disappeared or improved in 15% of the patients.
SUBUNGUAL AMELANOTIC MALIGNANT MELANOMA
Authors: Nakamura Shinichi , Nishihara Kiyoshi , Hoshi Kenji , Itoh Fumiyuki , Nakayama Keiji 
Published: April 2015

ABSTRACTA 52‐year‐old man with subungual amelanotic malignant melanoma was reported. He had a dome‐shaped tumor on his right thumb nail bed without any involvement. Clinically, we considered various diseases including amelanotic malignant melanoma, squamous cell carcinoma, granuloma pyogenicum, and other granulomas. Histological features of the skin biopsy taken during the first visit suggested squamous cell carcinoma. We still considered the possibility of amelanotic malignant melanoma, however, and the specimens taken during the radical operation were examined histologically and histochemically and a diagnosis of subungual amelanotic malignant melanoma was determined.
The Inhibitory Effect of Selenium on Mice Inoculated with B16 Melanoma Cells
Authors: Hanada Katsumi , Satoh Shizuo , Sawamura Daisuke , Hashimoto Isao , Katabira Yasuo 
Published: April 2015

AbstractThe influence of selenium dioxide on the in vitro and in vivo growth of transplantable B16 melanoma was studied. In the in vivo study, melanoma tissues showed a decrease in development of tumor growth and a tendency toward higher levels of free radical signal (ESR). Cultured B16 melanoma cells revealed a marked disturbance of cell growth especially in the solution with a high dose of selenium.These results may indicate that selenium has an antitumorigenic effect on B16 melanoma cells.
Sweat Gland Necrosis in Drug‐Induced Coma
Authors: Rocamora Antonio , Matarredona Jaime , Sendagorta Enrique , Ledo Antonio 
Published: April 2015

AbstractWe report herein characteristic traumatic skin lesions with blisters and sweat gland necrosis in a case of drug‐induced coma in which direct immunofluorescence demonstrated deposits of immunoglobulin and C3 in dermal vessels. The significance of our direct immunofluorescence findings is discussed.
Dyskeratosis Congenita
Authors: Singh Kamlender , Kolalapudi Seetharamanjaneyule 
Published: April 2015

AbstractA case of dyskeratosis congenita has been documented for the first time in an Indian patient. It began soon after birth with the formation of bullae at traumatic sites, masquerading as epidermolysis bullosa. Nail changes and pigmentary changes appeared simultaneously at the age of 4 years. This seems to be the seventh reported case resulting from a fourth consanguineous marriage.
A Case of Male SLE—With Special Reference to Familial Occurrence and HLA
Authors: Maki Tokiyo , Saida Yoko 
Published: April 2015

AbstractA male case of SLE who showed the female pattern of clinical findings was reported with special reference to his family history and HLA typing. One of the HLA haplo‐types of the patient corresponded to that of his sister, who had an identical disease. The result of this study suggests that genetic factors other than the HLA system might play roles in the development of SLE.
Neuron‐Specific Enolase (NSE): A Specific Marker for Merkel Cells in Human Epidermis
Authors: Masuda Tetsuo , Ikeda Shigeo , Tajima Kimiko , Kawamura Taro 
Published: April 2015

AbstractClear cells aggregated on the tip of crista profunda intermedia (CPI) are deduced to be Merkel cells (MK) by summarizing the results reported by previous authors. Among epidermal clear cells, only MKs stain positively with the Abidin‐Biotin‐Peroxydase Complex (ABC) method for neuron‐specific enolase (NSE).
Organ Culture of Skin of Rabbits and Guinea Pigs: Maintenance of Complete Keratinization and Effect of Lithium on Keratinization
Authors: Kondo Shigeo , Hozumi Yutaka 
Published: April 2015

AbstractA method for maintaining epidermal structure without causing initial parakeratosis or necrosis in organ culture of skin explants was described. Split thickness skin specimens from the ears of rabbits and guinea pigs were put in diffusion chambers made of millipore filters, which were then submitted to a roller tube culture at 15 rpm and 32°C. The culture medium was Eagle's minimum essential medium with Hanks salts supplemented with 20% fetal calf serum. The gas phase was a critical factor for the culture: in air + 5% CO2 (gas phase A), the rabbit skin explant (thin epidermis) was maintained without epidermal parakeratosis or necrosis for about 14 days, while the guinea pig skin explant (thick epidermis) tended towards its epidermal parakeratosis or necrosis. In two other gas phases, 50% O2 + 45% N2 + 5% CO2 (gas phase B), and 95% O2 + 5% CO2 (gas phase C), explants from the two animals were well maintained without epidermal parakeratosis or necrosis for about 14 days. The addition of LiCl (≧1 mM) caused the skin specimens of both animals to become parakeratotic; 10 μg/ml dexamethasone and 10 mM KCl antagonized the LiCl effect but 10 mM MgCl2, 0.1 mM N6, 2′‐O‐dibutyryladenosine 3′:5′‐cyclic monophosphate (db cAMP) and 0.1 mM N6, 2′‐O‐dibutyrylguanosine 3′:5′‐cyclic monophosphate (db cGMP) did not.
A Simplified Disc Technique for Quantitating Mouse DNA, RNA and Protein Synthesis: Circadian Rhythms
Authors: Yoshino Kazuhiro , Oba Kenkichi , Maibach Howard I. 
Published: April 2015

Abstract1) A modified disc technique has been developed for quantitating DNA, RNA and protein synthesis in epidermal hairless mouse skin and utilized in examining circadian rhythms. 2) The modified epidermal assay for DNA synthesis, utilizing dissolution of epidermis before scintillation counting, yields three times the specific activity than does the original disc technique. The modified disc technique also gives satisfactory results for RNA and protein synthesis. 3) The incorporation of 3H‐thymidine (3H‐TdR) into DNA was highest from 23:00 to 03:00. RNA synthesis, as measured by 3H‐uridine (3H‐UR) incorporation, and protein synthesis, as measured by 3H‐leucine (3H‐Leu) and 14C‐histidine (14C‐His) incorporation, did not show any obvious circadian rhythm.
Prognostic Significance of Initial Skin Lesions in Epidermotropic Cutaneous Lymphoma
Authors: Yamamura Teiichi , Yoshikawa Kunihiko , Aozasa Katsuyuki 
Published: April 2015

AbstractThe prognostic significance of initial skin lesions was studied in thirty cases of epidermotropic cutaneous lymphoma. The numbers of patients with initial erythema, papules, or nodule or tumors were 22 (72%)‐Group I, 6 (20%)‐Group II, and 2 (7%)‐Group III, respectively. Nodules or tumors developed earlier in Group II patients than in Group I patients; the percentages of tumor death were 83% in the former and 25% in the latter. The Group I patients had a more favorable 2‐year survival rate (72%) than did the Group II patients (0%) (p<0.025). It is suggested that the macroscopic appearance of initial skin lesions, either erythema or papules, is a useful prognostic marker.
Effect of Plant Worm Extract on Mouse Hair Growth
Authors: Ogawa Hideoki , Ogura Kyoichi , Ishigouoka Hiroshi , Suwa Yoshihide , Tanaka Takaharu , Yoshizumi Hajime 
Published: April 2015

AbstractTopical application of plant worm extract (Chinese drug, dong chong xia cao) caused a dose‐dependent acceleration of hair growth on the dorsal resting hair of shaved C3H mice. The original extract, which was extracted from 3 g of plant worm with 400 ml of 75% ethanol, and a 10‐fold concentrate were significantly effective in accelerating hair regrowth. Even a 100‐fold diluted extract shortened the anagen time of terminal hair growth. The hair regrowth activity of the extract was also highly significant when compared with two solutions containing some other effective ingredients for hair regrowth. The extract did not show any apparent acute toxicity in the mice. No mutagenicity was detected. No systemic or local side effects attributable to the plant worm extract were evident.
Hirsutoid Papillomas of Vulvae
Authors: Kohda Hiromu , Hino Yuwao , Fukuda Hidezo 
Published: April 2015

AbstractCondylomata acuminata‐like lesions of the vulvae were observed in a 28‐year‐old woman. They were located on the interior borders of the labia minora and were filiform, smooth, flesh‐coloured and asymptomatic. Histologically, these lesions showed a rich vascular network surrounded by connective tissue. They appear to be identical to hirsutoid papillomas of the glans penis, as they share the same embryological location. To our knowledge, this is the first report of condylomata acuminata‐like lesions of the labia minora in Japan.
Circulating Immune Complexes in Recurrent Oral Ulcers
Authors: Sun Andy , Wu Ying‐Chin , Liang Lu‐Chuan , Kwan Hsueh‐Wan 
Published: April 2015

AbstractSerum immunoglobulins (IgG, M, A), serum complements (C3, C4) and circulating immune complexes (CIC) were measured in forty‐five patients with recurrent oral ulcers (ROU) and in forty‐one healthy controls. The serum levels of immunoglobulins in patients with ROU were significantly higher than normal values. Similar to the pattern during infections, the levels of IgM and IgA gradually decreased as the disease entered the late stage and then returned to normal; the IgG level remained significantly high in the convalescent stage.Although the serum complements were not depressed throughout the course of the disease, a significant amount of CIC was detected in various stages and types of ROU. The three stages of ROU showed an increase in the abnormal percentage of CIC in this order: active early stage (3/14; 21.4%), active late stage (15/39; 38.5%) and convalescent stage (6/15; 40%). Although all three types of ROU demonstrated the variable positivity of CIC [minor, 16/31 (51.6%); major, 3/8 (37.5%); and herpetiform, 3/6 (50%)], their differences were not significant stastistically. This study demonstrated an aberration of humoral immunity of a rather non‐specific nature in ROU, probably caused by an infective process. This may suggest that CIC plays only a secondary role in the pathogenesis of ROU.