bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strength through Weakness: State Executive Power and Federal Reform in Austria
Authors: Karlhofer Ferdinand , Pallaver Günther 
Published: March 2013

AbstractDue to the absence of a strong constitutional veto player, comparative research uses to classify Austria as country with weak federal structures, occasionally even as a “federation without federalism”. From an institutional perspective, the assessment is definitely correct, in particular with regard to the nearly insignificant status of the second chamber Bundesrat. Apart from constitutional provisions, however, there are informal forces at work in decision‐making processes. Our thesis is that any approach ignoring the fact that the nominal constitution is paralleled by a real one falls short. Instead, we focus on the Conference of State Governors which, though not established by law, is a strong player in Austria's multi‐layered system qualifying the picture of “weak” federalism. The paper gives on overview of origin and function of the conference and its ambivalent role in making up for the shortcomings of the federal structures. Taking recent attempts of reorganizing fiscal equalization between states and federation as an example, the need of reforming the allocation of rights and duties between the different levels and, at the same time, the obstacles blocking the reform is outlined and analyzed.
Does Participation in Policymaking Enhance Satisfaction with the Policy Outcome? Evidence from Switzerland
Authors: Traber Denise 
Published: March 2013

AbstractThis article investigates the benefit of participation in policymaking. Based on data from interviews with various interest groups, private organisations, and representatives of regions that had been involved in recent policymaking processes in Switzerland, this study analyses the relationship between participation and satisfaction with the policy outcome. The study looks at two different aspects of outcome satisfaction: perceived consensus of a decision and preference attainment. The results show that the level of participation is related to both aspects of outcome satisfaction but that the strength of association is dependent on the type of interest group and the level of conflict among the groups participating in the decision‐making process.
Serum CCL11 (eotaxin‐1) and CCL17 (TARC) are serological indicators of multiple helminth infections and are driven by Schistosoma mansoni infection in humans
Authors: Geiger Stefan M. , Jardim‐Botelho Anne , Williams Weston , Alexander Neal , Diemert David J. , Bethony Jeffrey M. 
Published: March 2013

AbstractObjectivesTo evaluate systemic serum cytokine and chemokine markers for inflammation and Th1/Th2 responses in relation to multiple helminth infections, parasite burden and/or nutritional status of individuals.MethodsIn a longitudinal study, stool samples from 210 individuals from an area highly endemic for Ascaris lumbricoides, Necator americanus and Schistosoma mansoni were examined before and 12 months after clearance of parasites by chemotherapy. On both occasions, the presence of mono‐ or multiple infections and intensities of infection were compared with nutritional parameters and with serum cytokines or chemokines as markers for inflammatory, regulatory or Th1‐ or Th2‐type immune responses.ResultsBefore treatment, we were not able to associate any altered nutritional parameters with increased inflammatory responses, and highest intensities of infection were found in eutrophic participants with multiple infections. In contrast, major changes in serum Th2‐type chemokine levels were measured in individuals infected with intestinal helminths and/or S. mansoni, and resulted in significantly higher CCL11 and CCL17 concentrations, both before treatment and after reinfection.ConclusionsThe driving force for these elevated type 2 serum chemokine concentrations was an S. mansoni infection and faecal egg counts significantly correlated with serum IL‐10 concentrations.
Singing Numbers… in Cognitive Space — A Dual‐Task Study of the Link Between Pitch, Space, and Numbers
Authors: Fischer Martin H. , Riello Marianna , Giordano Bruno L. , Rusconi Elena 
Published: March 2013

AbstractWe assessed the automaticity of spatial‐numerical and spatial‐musical associations by testing their intentionality and load sensitivity in a dual‐task paradigm. In separate sessions, 16 healthy adults performed magnitude and pitch comparisons on sung numbers with variable pitch. Stimuli and response alternatives were identical, but the relevant stimulus attribute (pitch or number) differed between tasks. Concomitant tasks required retention of either color or location information. Results show that spatial associations of both magnitude and pitch are load sensitive and that the spatial association for pitch is more powerful than that for magnitude. These findings argue against the automaticity of spatial mappings in either stimulus dimension.
Evaluating ecological‐niche factor analysis as a modelling tool for environmental weed management in island systems
Authors: Costa H , Medeiros V , Azevedo E B , Silva L 
Published: March 2013

SummaryManagement actions are essential for mitigating the potentially harmful changes in biodiversity, ecosystem function and crop/forest productivity caused by invasive species. Species distribution models, if reliable, could be used to design effective management strategies. Although several modelling methods well suited for studying invasive species have been developed for presence‐only data, often the size of available data sets for modelling is small and results are not validated with test samples. Moreover, the impact of such methods in practical applications has been overlooked. Here, we evaluated the reliability of the modelling approach based on ecological‐niche factor analysis (ENFA) implemented in Biomapper software when applied to environmental weed data in the Azores. Presence‐only data sets of two top invasive woody species (Pittosporum undulatum and Acacia melanoxylon) were used. The continuous Boyce curve was used for validation, calculated either in Biomapper (cross‐validation) or based on test samples. The species' most habitable areas that should be regarded as management targets were thus estimated from modelling and validation. By imposing size restrictions on the presence‐only data sets used in modelling and validation, other habitable areas were defined and compared. The ENFA proved to be a suitable method for modelling environmental weed distributions, regardless of the presence‐only dataset size. Moreover, the cross‐validation of Biomapper was reliable, although its results should be interpreted with caution as they could potentially lead to statistically different management target areas.
Draft Genome Sequence of Streptomyces bottropensis ATCC 25435, a Bottromycin-Producing Actinomycete
Authors: Zhang Hongyu , Zhou Wei , Zhuang Yibin , Liang Xiaomei , Liu Tao 
Published: March 2013

ABSTRACT

A series of bottromycin antibiotics have been isolated and identified from
Streptomyces bottropensis
strain ATCC 25435. Here, a draft genome sequence of
S. bottropensis
ATCC 25435 is presented. The genome carries an intact biosynthetic gene cluster for bottromycin antibiotics, which provides insight into the combinatorial biosynthesis of bottromycin antibiotics.
Whole-Genome Sequence of the Fish Virulent Strain Streptococcus iniae IUSA-1, Isolated from Gilthead Sea Bream (Sparus aurata) and Red Porgy (
Pagrus pagrus
)
Authors: El Aamri Fatima , Acosta Félix , Real Fernando , Padilla Daniel 
Published: March 2013

ABSTRACT

Streptococcus iniae
is a major fish pathogen that produces invasive infections that result in economic losses in aquaculture. In this study, the draft genome sequence of
Streptococcus iniae
strain IUSA-1, isolated from a natural outbreak affecting gilthead sea bream (
Sparus aurata
) and red porgy (
Pagrus pagrus
), is presented.
Complete Genome of the Solvent-Tolerant Staphylococcus warneri Strain SG1
Authors: Cheng Victor W. T. , Zhang Guijin , Oyedotun Kayode S. , Ridgway Doug , Ellison Michael J. , Weiner Joel H. 
Published: March 2013

ABSTRACT

Staphylococcus warneri
is a Gram-positive bacterium commonly found in human skin flora. The genome of a laboratory
S. warneri
isolate, strain SG1, was sequenced to explore its mechanism of solvent tolerance and its potential as a chassis for biofuel production.
Genome Sequence of the Group III Clostridium botulinum Strain Eklund-C
Authors: Hassan Karl A. , Tetu Sasha G. , Elbourne Liam D. H. , Johnson Eric A. , Paulsen Ian T. 
Published: March 2013

ABSTRACT

The neurotoxins produced by
Clostridium botulinum
strains are among the world's most potent toxins and are the causative agents of paralytic botulism. Here, we present the draft genome sequence of the group III
C. botulinum
strain Eklund-C, including a pseudolysogen-like bacteriophage that harbors the type C neurotoxin operon.
Complete Genome Sequence of a Hemagglutination-Negative Avian Paramyxovirus Type 4 Isolated from China
Authors: Wang Kai-Cheng , Chen Gui-Qian , Jiang Wen-Ming , Liu Shuo , Hou Guang-Yu , Yu Jian-Min , Li Jin-Ping , Zhuang Qing-Ye , Chen Ji-Ming 
Published: March 2013

ABSTRACT
We report here the complete genome sequence of a nonpathogenic and hemagglutination-negative avian paramyxovirus type 4 isolated from a duck in southern China. Phylogenetic analysis of the genome sequence indicated that the waterfowl virus possibly has evolved into the Eastern and Western Hemisphere lineages.
Acheta domesticus
Volvovirus, a Novel Single-Stranded Circular DNA Virus of the House Cricket
Authors: Pham Hanh T. , Bergoin Max , Tijssen Peter 
Published: March 2013

ABSTRACT
The genome of a novel virus of the house cricket consists of a 2,517-nucleotide (nt) circular single-stranded DNA (ssDNA) molecule with 4 open reading frames (ORFs). One ORF had a low identity to circovirus nucleotide sequences (NS). The unique properties of this volvovirus suggested that it belongs to a new virus family or genus.
Complete Genome Sequence of Bacillus thuringiensis subsp.
kurstaki
Strain HD73
Authors: Liu Guiming , Song Lai , Shu Changlong , Wang Pinshu , Deng Chao , Peng Qi , Lereclus Didier , Wang Xumin , Huang Dafang , Zhang Jie , Song Fuping 
Published: March 2013

ABSTRACT

Bacillus thuringiensis
is a Gram-positive bacterium that produces intracellular protein crystals toxic to a wide variety of insect larvae. We report the complete genome sequence of
Bacillus thuringiensis
subsp.
kurstaki
strain HD73 from the Centre OILB (Institut Pasteur, France), which belongs to serotype 3ab and is toxic to lepidopteran larvae.
Draft Genome Sequence of
Frankia
sp. Strain CN3, an Atypical, Noninfective (Nod
–
) Ineffective (Fix
–
) Isolate from
Coriaria nepalensis
Authors: Ghodhbane-Gtari Faten , Beauchemin Nicholas , Bruce David , Chain Patrick , Chen Amy , Walston Davenport Karen , Deshpande Shweta , Detter Chris , Furnholm Teal , Goodwin Lynne , Gtari Maher , Han Cliff , Han James , Huntemann Marcel , Ivanova Natalia , Kyrpides Nikos , Land Miriam L. , Markowitz Victor , Mavrommatis Kostas , Nolan Matt , Nouioui Imen , Pagani Ioanna , Pati Amrita , Pitluck Sam , Santos Catarina L. , Sen Arnab , Sur Saubashya , Szeto Ernest , Tavares Fernando , Teshima Hazuki , Thakur Subarna , Wall Luis , Woyke Tanja , Tisa Louis S. 
Published: March 2013

ABSTRACT

We report here the genome sequence of
Frankia
sp. strain CN3, which was isolated from
Coriaria nepalensis
. This genome sequence is the first from the fourth lineage of
Frankia
, strains of which are unable to reinfect actinorhizal plants. At 10 Mb, it represents the largest
Frankia
genome sequenced to date.
Complete Genome of
Serratia
sp. Strain FGI 94, a Strain Associated with Leaf-Cutter Ant Fungus Gardens
Authors: Aylward Frank O. , Tremmel Daniel M. , Starrett Gabriel J. , Bruce David C. , Chain Patrick , Chen Amy , Davenport Karen W. , Detter Chris , Han Cliff S. , Han James , Huntemann Marcel , Ivanova Natalia N. , Kyrpides Nikos C. , Markowitz Victor , Mavrommatis Kostas , Nolan Matt , Pagani Ioanna , Pati Amrita , Pitluck Sam , Teshima Hazuki , Deshpande Shweta , Goodwin Lynne , Woyke Tanja , Currie Cameron R. 
Published: March 2013

ABSTRACT

Serratia
sp. strain FGI 94 was isolated from a fungus garden of the leaf-cutter ant
Atta colombica
. Analysis of its 4.86-Mbp chromosome will help advance our knowledge of symbiotic interactions and plant biomass degradation in this ancient ant-fungus mutualism.
Fatal road traffic injuries in Ibadan, using the mortuary as a data source
Authors: Eze Uwom O , Kipsaina Chebiwot Caroline , Ozanne-Smith Joan 
Published: March 2013

BackgroundRoad Traffic Injury (RTI) in Africa represents 14% of global RTI deaths. Lack of timely, reliable data undermines road safety interventions. Available fatality data are aggregated, limited in detail or scarce in surveys. This is the first fatal RTI surveillance study in Nigeria.ObjectiveTo pilot a systematic mortuary-based data collection in Ibadan, determine the nature and circumstances of fatal RTI and assess data quality against existing data sources.MethodsUsing a draft data collection system developed jointly by WHO and Monash University, the detailed information was prospectively collected on RTI University College Hospital mortuary admissions in Ibadan September 2010 to February 2011. Demographics, road user type, counterpart vehicle, intent, manner and medical cause of death were recorded.ResultsMortuary admissions included 80 fatal RTI cases: 81.3% males. By road user category, 28 (35.0%) were pedestrians; 28 (35.0%) motorised 2-wheeler users; 18.8% car occupants; and 11.3% bus occupants. In 70% of cases, medical cause of death was head injury, including 25 of 28 motorised 2-wheeler users (89.3%). Estimates from this study indicate apparent increased mortuary capture of fatal RTI compared with police data.ConclusionsThis study demonstrates the feasibility of collecting detailed, timely RTI fatality data through mortuary-based surveillance in Ibadan. While not all RTI deaths are reported to any authority in Ibadan, this large case series complements existing data sources and suggests that pedestrians and motorised 2-wheeler users die most often in road traffic crashes. Frequent head injuries among motorised 2-wheeler users strongly support the need for helmet wearing interventions.
Dachshund Binds p53 to Block the Growth of Lung Adenocarcinoma Cells
Authors: Chen Ke , Wu Kongming , Cai Shaoxin , Zhang Wei , Zhou Jie , Wang Jing , Ertel Adam , Li Zhiping , Rui Hallgeir , Quong Andrew , Lisanti Michael P. , Tozeren Aydin , Tanes Ceylan , Addya Sankar , Gormley Michael , Wang Chenguang , McMahon Steven B. , Pestell Richard G. 
Published: March 2013

Abstract
Hyperactive EGF receptor (EGFR) and mutant p53 are common genetic abnormalities driving the progression of non–small cell lung cancer (NSCLC), the leading cause of cancer deaths in the world. The Drosophila gene Dachshund (Dac) was originally cloned as an inhibitor of hyperactive EGFR alleles. Given the importance of EGFR signaling in lung cancer etiology, we examined the role of DACH1 expression in lung cancer development. DACH1 protein and mRNA expression was reduced in human NSCLC. Reexpression of DACH1 reduced NSCLC colony formation and tumor growth in vivo via p53. Endogenous DACH1 colocalized with p53 in a nuclear, extranucleolar location, and shared occupancy of −15% of p53-bound genes in ChIP sequencing. The C-terminus of DACH1 was necessary for direct p53 binding, contributing to the inhibition of colony formation and cell-cycle arrest. Expression of the stem cell factor SOX2 was repressed by DACH1, and SOX2 expression was inversely correlated with DACH1 in NSCLC. We conclude that DACH1 binds p53 to inhibit NSCLC cellular growth. Cancer Res; 73(11); 3262–74. ©2013 AACR.
Sequential Binary Gene Ratio Tests Define a Novel Molecular Diagnostic Strategy for Malignant Pleural Mesothelioma
Authors: De Rienzo Assunta , Richards William G. , Yeap Beow Y. , Coleman Melissa H. , Sugarbaker Peter E. , Chirieac Lucian R. , Wang Yaoyu E. , Quackenbush John , Jensen Roderick V. , Bueno Raphael 
Published: March 2013

Abstract
Purpose: To develop a standardized approach for molecular diagnostics, we used the gene expression ratio bioinformatic technique to design a molecular signature to diagnose malignant pleural mesothelioma (MPM) from among other potentially confounding diagnoses and differentiate the epithelioid from the sarcomatoid histologic subtype of MPM. In addition, we searched for pathways relevant in MPM in comparison with other related cancers to identify unique molecular features in MPM.
Experimental Design: We conducted microarray analysis on 113 specimens including MPMs and a spectrum of tumors and benign tissues comprising the differential diagnosis of MPM. We generated a sequential combination of binary gene expression ratio tests able to discriminate MPM from other thoracic malignancies. We compared this method with other bioinformatic tools and validated this signature in an independent set of 170 samples. Functional enrichment analysis was conducted to identify differentially expressed probes.
Results: A sequential combination of gene expression ratio tests was the best molecular approach to distinguish MPM from all the other samples. Bioinformatic and molecular validations showed that the sequential gene ratio tests were able to identify the MPM samples with high sensitivity and specificity. In addition, the gene ratio technique was able to differentiate the epithelioid from the sarcomatoid type of MPM. Novel genes and pathways specifically activated in MPM were identified.
Conclusions: New clinically relevant molecular tests have been generated using a small number of genes to accurately distinguish MPMs from other thoracic samples, supporting our hypothesis that the gene expression ratio approach could be a useful tool in the differential diagnosis of cancers. Clin Cancer Res; 19(9); 2493–502. ©2013 AACR.
Targeted Delivery of microRNA-29b by Transferrin-Conjugated Anionic Lipopolyplex Nanoparticles: A Novel Therapeutic Strategy in Acute Myeloid Leukemia
Authors: Huang Xiaomeng , Schwind Sebastian , Yu Bo , Santhanam Ramasamy , Wang Hongyan , Hoellerbauer Pia , Mims Alice , Klisovic Rebecca , Walker Alison R. , Chan Kenneth K. , Blum William , Perrotti Danilo , Byrd John C. , Bloomfield Clara D. , Caligiuri Michael A. , Lee Robert J. , Garzon Ramiro , Muthusamy Natarajan , Lee Ly James , Marcucci Guido 
Published: March 2013

Abstract
Purpose: miR-29b directly or indirectly targets genes involved in acute myeloid leukemia (AML), namely, DNMTs, CDK6, SP1, KIT, and FLT3. Higher miR-29b pretreatment expression is associated with improved response to decitabine and better outcome in AML. Thus, designing a strategy to increase miR-29b levels in AML blasts may be of therapeutic value. However, free synthetic miRs are easily degraded in bio-fluids and have limited cellular uptake. To overcome these limitations, we developed a novel transferrin-conjugated nanoparticle delivery system for synthetic miR-29b (Tf-NP-miR-29b).
Experimental Design: Delivery efficiency was investigated by flow cytometry, confocal microscopy, and quantitative PCR. The expression of miR-29b targets was measured by immunoblotting. The antileukemic activity of Tf-NP-miR-29b was evaluated by measuring cell proliferation and colony formation ability and in a leukemia mouse model.
Results: Tf-NP-miR-29b treatment resulted in more than 200-fold increase of mature miR-29b compared with free miR-29b and was approximately twice as efficient as treatment with non-transferrin–conjugated NP-miR-29b. Tf-NP-miR-29b treatment significantly downregulated DNMTs, CDK6, SP1, KIT, and FLT3 and decreased AML cell growth by 30% to 50% and impaired colony formation by approximately 50%. Mice engrafted with AML cells and then treated with Tf-NP-miR-29b had significantly longer survival compared with Tf-NP-scramble (P = 0.015) or free miR-29b (P = 0.003). Furthermore, priming AML cell with Tf-NP-miR-29b before treatment with decitabine resulted in marked decrease in cell viability in vitro and showed improved antileukemic activity compared with decitabine alone (P = 0.001) in vivo.
Conclusions: Tf-NP effectively delivered functional miR-29b, resulting in target downregulation and antileukemic activity and warrants further investigation as a novel therapeutic approach in AML. Clin Cancer Res; 19(9); 2355–67. ©2013 AACR.
15-Lipoxygenase Promotes Chronic Hypoxia–Induced Pulmonary Artery Inflammation via Positive Interaction With Nuclear Factor-κB
Authors: Li Jing , Rao Jingjing , Liu Yun , Cao Yonggang , Zhang Yueming , Zhang Qianlong , Zhu Daling 
Published: March 2013

Objective—
Our laboratory has previously demonstrated that 15-lipoxygenase (15-LO)/15-hydroxyeicosatetraenoic acid (15-HETE) is involved in hypoxic pulmonary arterial hypertension. Chronic hypoxia–induced vascular inflammation has been considered as an important stage in the development of pulmonary arterial hypertension. Here, we determined the contribution of 15-HETE in the hypoxia–induced pulmonary vascular inflammation.

Approach and Results—
Chronic hypoxia–induced monocyte/macrophage infiltration and the expressions of intercellular adhesion molecule-1 and vascular cell adhesion molecule-1 were analyzed in hypoxic rat model and cultured pulmonary arterial endothelium cells using immunochemistry methods. We found that monocyte/macrophage infiltration and the expressions of intercellular adhesion molecules under hypoxia were markedly inhibited by 15-HETE inhibitors or 15-LO1/2 small interfering RNA. In addition, exogenous 15-HETE enhanced the expression of both adhesion molecules in pulmonary arterial endothelium cells in a time-dependent manner. Hypoxia–induced 15-LO1/2 expression in rat pulmonary arterial endothelium cells was significantly abolished by nuclear factor-κB inhibitors. Meanwhile, nuclear factor-κB activity was enhanced prominently by the 15-LO1/2 product, 15-HETE, suggesting a positive feedback mechanism.

Conclusions—
Taken together, our results suggest that chronic hypoxia promotes monocyte infiltration into the vasculature and adhesion molecules upregulation in pulmonary arterial endothelium cells via a positive interaction between 15-LO/15-HETE and nuclear factor-κB. Our study revealed a novel mechanism underlying hypoxia–induced pulmonary arterial inflammation and suggested new therapeutic strategies targeting 15-LO/15-HETE and nuclear factor-κB in the treatment of pulmonary arterial hypertension.