bluuebunny/crossref_metadata_2025_split huggingface dataset:
Partial purification and characterization of bacteriocin from novel Brevibacillus borstelensis sp. isolated from Donkey’s lactation
Authors: Kumari Ankur , Sharma Parvati , Bhardwaj Anuradha , Pal Yash 
Published: June 2024

The heightened awareness of ensuring enhanced safety standards is growing in order pertaining to the avoidance of chemical food preservation. Concurrently, there is a discernible increase in the prevalence of antimicrobial resistance among foodborne pathogens, which engenders infections such as food poisoning, gastro-intestinal infections etc.. In this regard, bacteriocins present themselves as a compelling alternative for achieving both preservation and safety objectives in the realm of food science. Bacteriocins are ribosomally synthesized proteins that demonstrate inhibitory activities against a diverse spectrum of undesirable microorganisms. In the present study, a bacterium (Brevibacillus borstelensis sp.) derived from Donkey milk was scrutinized for its ability to generate a bacteriocin-like inhibitory substance (BLIS). The effectiveness of this substance was assessed against pathogens linked to foodborne/spoilage, specifically Bacillus subtilis (KK01), Staphylococcus aureus (MRSA) ,Pseudomonas aeruginosa (HCS36), and Escherichia coli (O22) .The evaluation comprised the bit/disc method followed by the Well-diffusion method. Employing a combination of phenotypic, biochemical, and molecular characteristics, including the 16S rRNA gene technique, the bacterial isolate was identified as Brevibacillus borstelensis strain 3.1 [OR272522.1]. The Bacteriocin-like inhibitory substance produced by B. borstelensiswas purified for the first time by using the chloroform extraction method, unveiling a molecular mass of 56 kDa. Further analysis examined the BLIS reactions to temperature, pH, proteolytic enzymes, and stability. The Bacteriocin-like inhibitory substance exhibited thermo-stability up to 90 °C, activity at neutral pH, sensitivity to trypsin, and partial stability up to 15 days of storage, indicating its potential as a bio-preservative for food.
Storing cork oak (Quercus suber) seeds under different temperatures and the effect of storage treatments on the germination and growth of stored seeds
Authors: Fargani Marwan YM El , Khaddari Amal El , Ouajdi Mohamed , Oubrahim Hayat , Aoujdad Jalila , El Antry Salwa , Dahmani Jamila , Zidane Lahcen 
Published: June 2024

The cork oak "Quercus suber L." is one of the most important forest trees in the world, especially in the Mediterranean Basin, due to its high environmental, socioeconomic, and landscape value throughout the year. In general, these trees face particular problems that affect their germination, growth, survival, and the other biotic and abiotic causes that affect natural regrowth.Thus, the most important of these problems is the annual irregularity in tree seed production. For this reason, The storage problems of this species are of concern to managers, who are using new alternative techniques to deal with the problem of the irregular annual production of oak seeds and to preserve them for as long as possible. The present work aimed to evaluate the effect of storing cork oak Quercus suber L. seeds at different temperatures and for different periods of up to 300 days on their vitality and ability to germinate. For this, the seeds were subjected to four storage parameters at four temperature levels: -0.5°C, -1°C, +4°C with thermotherapy, and +4°C without thermotherapy as a control from January 2019 to September 2019. Analysis of the obtained results showed that the treatments studied significantly affected the measured parameters. After 30 and 60 days, the results of the 4 treatments concluded that the fourth storage treatment (storage of seeds directly at +4°C) presented a good performance in terms of the length of the seedlings, which was high (52.45 cm), the production of seedlings with an average diameter of 5.28 mm, and the important germination percentage (88.57%), unlike the other treatments. After a storage period of 270 days, the fourth storage treatment, the control, stored at 4°C without thermotherapy, still showed the best growth and development. This treatment also achieved a higher germination percentage (17.14%), plant height (11.33cm), and average diameter (1.85 mm) than the other treatments.
Applied fixed effect of Geographically Weighted Panel Regression (GWPR) with M- Estimator approach to estimate sugarcane yield data in East Java
Authors: Mondiana Yani Quarta , Pramoedyo Henny , Iriany Atiek , Marjono  
Published: June 2024

Geographically Weighted Panel Regression (GWPR), a combination of panel regression and geographically weighted regression (GWR), is used to analyze panel data and capture diverse relationship between locations. GWPR was developed on data with panel-fixed effects and applied to modeling data with spatial heterogeneity and time series. One method for estimating parameters in the GWPR is weighted least squares (WLS), which are sensitive to outliers. The present study aimed to use the M- method to estimate GWPR model parameters in data containing outliers using fixed-effect GWPR modeling for the sugar cane yield in East Java of Indonesia from 2019 to 2021. Sugarcane yield data in East Java contained outliers in several areas, including Malang, Blitar, and Ngawi Districts. Because the data contains outliers, a robust method with the M estimator was applied. The results showed that plantation areas significantly affected production in all districts.The R2 of the model was 0.87, showing that GWPR model with M estimation was appropriate in predicting sugarcane yield. Based on the Akaike Information Criterion (AIC) value, the GWPR model with M estimation had better performance than GWPR model alone.
Taxonomic redescription of subfamily Scymninae (Coleoptera: Coccinellidae) from Haryana, India
Authors: Singh Vijay , Grewal Sunita , Goyal Vivek , Malik Vinay 
Published: June 2024

The ladybird beetles of Scymninae, a subfamily of Coccinellidae are efficient biocontrol agents. Many earlier studies document their protective role associated with many crops. This subfamily is represented by 5 tribes,15 genera and 138 species in the Indian subcontinent. However there is no scientific record of taxonomic description of the subfamily Scymninae within the Coccinellidae family of beetles (Coccinellidae: Coleoptera) in Haryana, India. In the present study, seven species from three genera and two tribes of sub-family Scymninae i.e. Nephus (Bipunctatus) bipunctatus (Kugelann,1794), Nephus regularis (Sicard), Scymnus (Pullus) coccivora Ramakrishna Ayyar, 1925, Scymnus (Pullus) latemaculatus Motschulsky, Scymnus (Scymnus) nubilus Mulsant, Scymnus (Pullus) posticalis Sicard and Stethorus aptus Kapur were identified. It included the generation of keys to the tribes, genera, subgenera and species found during the study. It provided detailed taxonomy of the identified species based on various morphological characteristics such as coxal lines, antennae, mandibles, and male and female genitalia. This study marks the first taxonomic exploration of species within the Scymninae subfamily in Haryana, India. It will significantly contribute to understanding the biodiversity of beetles in the region and will lay the groundwork for further research and conservation initiatives.
PM2.5 concentration prediction using Generative adversarial network: A novel approach
Authors: Medhi Shrabani , Gogoi Minakshi 
Published: June 2024

Over the last few years, air pollution has become a matter of great concern. Numerous machine learning and deep learning techniques have been applied to predict PM2.5 (Particulate Matter2.5). However, deterministic models perform forecasting based on the mean of probable outputs and cannot handle the uncertainties in real-life situations. With the aim of solving the low accuracy of PM2.5 concentration prediction during uncertainties, the present study proposed an innovative probabilistic model-Prob PM2.5 which predicts one day ahead PM2.5 concentration for time series data, which is multivariate in nature. First, a comprehensive correlation analysis between the meteorological features and PM2.5 concentration is done. Finally, the Conditional GAN framework is used to train the ProbPM2.5 with the help of adversarial training. The proposed framework that transformed a deterministic model into a probabilistic model provided improved performance. Comparative analysis with conventional models, such as LSTM (Long Short-Term Memory) and GRU (Gated Recurrent Unit) reveals that ProbPM2.5 outperforms during testing, showcasing resilience in the face of unforeseen events like COVID-19. Hence, the proposed method could perform improved characterization of time series characteristics of the air pollutant changes in order to obtain better accuracy of PM2.5 concentration prediction
Study on soil organic carbon dynamics and its different pools in maize crop (Zea mays L.) under different agricultural practices in semiarid region of Punjab, India
Authors: Jackson Khaidem , Changade Nitin , Meetei Thounaojam Thomas , Devi Heisnam Sobhana , Devi Yumnam Bijilaxmi , Bokado Kangujam 
Published: June 2024

The soil organic carbon (SOC) is a vital resource whose presence or absence can determine the quality of soils. The sustainability potential of soils can be unlocked with the presence of SOC. The present study aimed to evaluate the implications of different agricultural field practices on the soil organic carbon, its various fractions, and the soil organic carbon stocks in the semiarid region of Phagwara (Punjab) with a maize variety (Suvarna NMH-589). Soil samples were collected from all the treatments (T0, T1, T2, T3, …, T9) at two soil depths, ‘a’ (0-15 cm) and ‘b’ (15-30 cm), during the experimental period of 2022-23 and 2023-24. Analyses were performed on the soils collected at 0 DAS (days after sowing), 45 DAS and 90 DAS. A pooled mean data of the analysis revealed that the total organic carbon (TOC) was maximum in the straw mulching treatment T1 (7.87, 9.40, 11.50, along with increasing DAS). Values of TOC ranged from 5.48-11.05 g kg-1 and 4.16-7.93 g kg-1 at the surface and subsurface layers during the experimental periods. The oxidizable soil organic carbon (SOC) ranged from 3.67-6.07 g kg-1 and 2.62-4.63 g kg-1 at soil depths ‘a’ and ‘b’, respectively. There was the suggestive notion that the incorporation of organic matter and its decomposition has a positive effect towards increasing the organic carbon content in soils. The SOC stocks also fluctuated in a range of 7.26- 11.51 Mg ha-1 and 9.69-19.23 Mg ha-1 at the different soil depths ‘a’ and ‘b’. Differential accumulation of biomass in the surface and subsurface layers was the driving factor for such a range in the values obtained. The carbon fractions also fluctuated during the experimental periods. It was concluded that different agricultural practices greatly influenced the organic carbon dynamics in soil. The agricultural practices that could boost SOC could improve crop productivity, improve nutrient transformation, and act as a sink for CO2 in the soil.
Identification of class I, II, and III Integron genes in multidrug-resistant Acinetobacter baumannii strains
Authors: Abed Esraa S. , Ali Munim R. 
Published: June 2024

Integrons are one genetic factor that can contribute to the high prevalence of antibiotic resistance among Acinetobacter baumannii isolates and their spread. This research aimed to study the prevalence of class 1,2 and 3 integron in A. baumannii isolates and their relatedness to virulence factors of antibiotics resistance. Seventy clinical isolates of A.baumannii were isolated from several sources such as blood, urinary tract infection (UTI), sputum, urine, and cerebrospinal fluid (CSF). First, the isolates were identified and characterized according to certain morphology, cultural and biochemical tests. Second genotypic ally Identification of the isolates was confirmed by Polymerase chain reaction (PCR), which was performed by housekeeping gene 16sRNA and by (blaOxa 51gene) for A. baumannii species. The antimicrobial susceptibility technique was evaluated through disk diffusion methods. These isolates resistant to various antibiotics were analyzed for integron class I, II and III content and sequences of the amplification products by PCR. The findings showed that the predominant A. baumannii isolates were multidrug resistant and they were most resistant to 15 antibiotics. The higher resistance of A. baumannii to Gatifloxacin and lower resistance to polymyxin. Sixty clinical Multidrug resistance (MDR) isolates of A. baumannii had class I integron and five class III integron, but class II integron was not detected in the isolates. This revealed that the dissemination of MDR among A. baumannii may be associated with the presence of integrons class I and class III. These data indicate that integrons are gene cassettes containing antibiotic-resistance genes that play a major role in the virulence characteristics of MDR and Extensively-drug resistance (XDR) in Acinetobacter baumannii.
Determining the vertebra for pedicle subtraction osteotomy in surgical correction for ankylosing spondylitis with thoracolumbar kyphosis
Authors: Li Xiongjie , Kim Yong-Chan , Kim Sung-Min , Hung Billy Francis , Lee Young-Jik 
Published: June 2024

OBJECTIVE
This study aimed to provide a method for determining the apical vertebra for pedicle subtraction osteotomy (PSO) in corrective surgery for patients with ankylosing spondylitis (AS) with thoracolumbar kyphosis (TLK).

METHODS
The medical records of AS patients with TLK who underwent PSO between May 2009 and August 2022 were retrospectively reviewed, and 235 patients were included in the study. Using the proposed method, choosing the vertebra based on Kim’s apex (KA), which is defined as the farthest vertebra from a line drawn from the center of the T10 vertebral body to the midpoint of the S1 upper endplate, the authors analyzed 229 patients with apices at T12, L1, or L2 (excluding L3 because of the small sample size, n = 6). They divided all patients into two groups. Group A (n = 144) underwent PSO at the KA vertebra, while group B (n = 85) underwent PSO at a different level. Demographic and radiological data, including sagittal spinopelvic parameters of the entire spine, were collected. An additional analysis was performed on patients with the same KA vertebra.

RESULTS
The vertebra distributions of patients based on KA were T12 (28 [12.2%]), L1 (119 [52.0%]), and L2 (82 [35.8%]). The corrections of sagittal vertical axis (SVA; 101.0 ± 48.5 mm vs 82.0 ± 53.8 mm, p = 0.010), global kyphosis (GK; 31.6° ± 10.0° vs 26.4° ± 10.5°, p = 0.005), and TLK (29.4° ± 10.2° vs 24.2° ± 12.9°, p = 0.012) in group A were significantly greater than those in group B, and there was no difference in the corrections of thoracic kyphosis (TK), lumbar lordosis, and pelvic incidence between the two groups. On further analysis, group A showed greater correction in TK (26.2° ± 13.7° vs 0.1° ± 8.1°, p = 0.013) for patients with T12 as the KA; greater improvements in SVA (101.5 ± 44.2 mm vs 73.4 ± 48.7 mm, p = 0.020), GK (30.6° ± 11.0° vs 25.0° ± 10.4°, p = 0.046), and TLK (32.6° ± 7.8° vs 26.7° ± 9.9°, p = 0.012) for those with L1 as the KA; and significant correction in TLK (30.0° ± 6.3° vs 4.3° ± 19.5°, p = 0.008) for patients with L2 as the KA, compared with group B.

CONCLUSIONS
PSO at the apical vertebra provides a greater degree of correction of sagittal imbalance. The proposed method, selecting the vertebra based on KA, is easily reproducible for determining the apex level in AS patients with TLK.
PENGUATAN LUMBUNG PANGAN MASYARAKAT MELALUI PROGRAM KEMITRAAN
Authors: Marina Ida , Dinar Dinar , Izzah Liffi Halimatul 
Published: June 2024

Tujuan dari penelitian ini untuk mengetahui : suatu hubungan partisipasi ketahanan pangan keluarga petani dengan anggota program lumbung pangan padi, dan hubungan ketahanan pangan keluarga petani dengan kemitraan anggota program lumbung pangan padi. Penelitian ini telah di laksanakan pada kelompok Lumbung Pangan di Desa Walahar Kecamatan Gempol Kabupaten Cirebon. Metode yang digunakan adalah metode penelitian kuantitatif, yaitu menggunakan teknik survey deskriptif. Untuk pengumpulan data yaitu menggunakan data primer yang diperoleh dengan wawancara petani, dan untuk data skunder diperoleh dari yang berkaitan dengan penelitian yaitu intansi. Uji Korelasi Kendall’s tau adalah uji yang digunakan untuk mengetahui ketahanan pangan yang menguatkan lumbung pangan terhadap keluarga petani. Hasil dari penelitian menunjukkan : hubungan antara partisipasi ketahanan pangan keluarga dengan anggota kelompok pada program lumbung pangan padi memiliki hubungan yang sedang dan nyata, yaitu memperoleh nilai koefisien korelasi Kendall Tau sebesar 0,483 dan untuk nilai Sig.(2-tailed) yaitu sebesar 0,002 lebih kecil dari 0,05. Dan untuk hubungan ketahanan pangan keluarga dengan kemitraan anggota kelompok dalam program lumbung pangan padi memiliki hubungan yang sedang dan nyata, yaitu memperoleh nilai koefisien korelasi Kendall Tau sebesar 0,446 dan untuk nilai Sig.(2-tailed) yaitu sebesar 0,005 lebih kecil dari 0,05.
Functional foods for cholesterol management: A comparison between the United States and Japan
Authors: Zhou Jiawei , Martirosyan Danik 
Published: June 2024

Cholesterol management is a cornerstone of cardiovascular disease prevention. Effective management of cholesterol
levels can significantly reduce the incidence of cardiovascular events and is a critical component of public health
strategies aimed at reducing morbidity and mortality associated with heart disease. Functional foods are known as
natural or processed foods containing active compounds in safe, effective amounts, which have been proven to offer
health benefits. In recent years, the role of functional foods in managing cholesterol levels has garnered significant
attention. These foods offer a promising avenue for non-pharmacological intervention, providing an accessible and
natural option for individuals seeking to improve their cholesterol profile through dietary choices. Including functional
foods in dietary recommendations reflects a broader shift towards preventive healthcare and personalized nutrition.
This paper aims to provide an in-depth analysis of the emerging role of functional foods in the management of
cholesterol, with a dual focus on the United States and Japan. By comparing the prevalence and modifiable risk factors
of high cholesterol, regulatory environments, market dynamics, consumer behaviors, and the scientific validity of health
claims associated with functional foods, this document seeks to uncover the nuances that shape the acceptance and
effectiveness of these foods in each cultural and regulatory context. Through this comparative lens, the paper intends
to identify best practices, challenges, and opportunities for the integration of functional foods into dietary strategies
for cholesterol management. Ultimately, this analysis is designed to inform stakeholders—including policymakers,
health professionals, and food industry players—of the potential for functional foods as a non-pharmacological
approach to improving public health outcomes related to cardiovascular disease. The goal is to contribute to the
discourse on preventive health care and personalized nutrition, providing a foundation for future research, innovation,
and policy development in the global landscape of functional foods and cholesterol management.

Keywords: Cholesterol Management, Functional Foods, FOSHU, Functional Food Definitions, FOSHU Approval Criteria
Reduksi Harmonik dengan Common Mode pada Inverter 500 Watt
Authors: Muhirno Muhirno , Setiawan Agus , Irwansyah Nurkahfi 
Published: June 2024

Agar tidak terputus dengan sumber energi listrik maka di perlukan sistem backup. Salah satunya adalah dengan sistem charging di saat PLN menyala dan menyimpannya di akumulator untuk di gunakan pada saat pemadaman oleh PLN. Namun pada saat charging dilakukan listrik yang di simpan ke akumulator berupa arus DC sehingga perlu di rubah kembali ke arus AC yaitu dengan inverter AC to DC. Sistem inverter menggunakan swiching yang menimbulkan harmonisa. Gangguan pada sistem tenaga listrik yang di akibatkan oleh distorsi gelombang dan arus di sebut harmonisa. Harmonisa bisa menyebabkan kerusakan gelombang sinusidal menjadi tidak sinusidal lagi yang di sebabkan oleh gelombang frekuensi tinggi yang merupakan kelipatan dari frekuensi fundamental. Untuk mereduksi harmonik adalah dengan pemasangan filter emi pada output inverter. Penelitian ini mengukur berapa nilai efektifitas pemasangan filter menggunakan software Labview 2014 pada inverter custom low inverter 500 watt dengan beban induktif, resistif, kapasitif menggunakan software labview dan instrument NI Mydaq. Hasil dari penelitian adalah di perlukan daya stanby/tanpa beban pada filter emi common mode sebesar 0.03 Amper pada tegangan 220VAC. Pada kondisi tanpa beban terjadi kenaikan pada THDv sebesar 0.03 dari sebelum filter 1.7% menjadi 1.739%, dan penurunan THDi 0.12 dari 1.311% menjadi 1.119%. Pada kondisi dengan beban induktif terjadi penurunan pada THDv sebesar 0.104 dari sebelum filter 1.202% menjadi 1.098%, dan THDi sebesar 0.099% dari sebelum filter 1.325% menjadi 1.226%. Pada kondisi dengan beban resistif terjadi penurunan pada THDv sebesar 0.12 dari sebelum filter sebesar 1.218% menjadi 1.09%, dan THDi sebesar 0.003 dari sebelum fiter 1.144% menjadi 1.114%. Pada kondisi dengan beban kapasitif terjadi kenaikan pada THDv sebesar 0.185 dari sebelum filter sebesar 1.219% menjadi 1.404% dan mengalami penurunan pada THDi sebesar 0.035 dari sebelum filter sebesar 1.44% menjadi 1.109%.
Kata Kunci: Filter Emi, Harmonisa, Inverter, LabView, Reduksi
The Strategy Of Puri Agung Tabanan As A Cultural Center
Authors: Antara I Wayan , Yudha Triguna Ida Bagus Gde , Bagus Wirawan I Gusti 
Published: June 2024

A Balinese culture is a form of culture in which every system of ideas, and social system to its physical manifestation in totality is based on values derived from Hinduism. Puri with its cultural resources still upholds the values of local wisdom of a region and represents the cultural and spiritual roots of Bali. In recent decades, puri as one of the elements of Balinese culture has been faced with various serious problems related to the existence of puri as a cultural center.
This research aims to examine the existence of Puri Agung Tabanan as a cultural center. Based on this, the researchers formulated three problems, namely: Why is Puri Agung Tabanan declared as a cultural center? What is the strategy of Puri Agung Tabanan as a cultural center? What are the implications of Puri Agung Tabanan as a cultural center for the existence of Balinese culture, the puri, and the surrounding community? This research uses a qualitative approach method, with descriptive analysis. Data collection techniques were observation, in-depth interviews, and document studies. Informants were collected through a purposive sampling technique. The research location is Puri Agung Tabanan located in Banjar Dinas Sakenan Baleran, Delod Peken Village, Tabanan District, precisely on Jalan Srigunting No. 3, Tabanan, Bali.
Formally, puri as the center of power is gone, but puri is symbolic. Puri’s strategy is to maintain its existence as a cultural center through acculturation or socialization strategy. The explanation model of Puri Agung Tabanan as a cultural center is a form of articulation that the function of the puri as the center of inheritance and preservation of Balinese culture remains relevant in the future. The existence of puri as a cultural center is a form of the birth of a new civilization milestone created by the current generation to represent the Balinese people, especially in Tabanan Regency in building a spirit based on a system of cultural values that already exists in the relationship of community life towards Bali New Era 100 years ahead (2025 to 2125).