bluuebunny/crossref_metadata_2025_split huggingface dataset:
Twenty years of herpes simplex virus type 2 (HSV-2) research in low-income and middle-income countries: systematic evaluation of progress made in addressing WHO prioritiesfor research in HSV-2 epidemiology and diagnostics
Authors: Jama Muna , Owen Ela Mair , Nahal Belinder , Obasi Angela , Clarke Emily 
Published: July 2024

IntroductionLow-income and middle-income countries (LMICs) have a high burden of herpes simplex virus type 2 (HSV-2) infection, which has been strongly associated with HIV. In 2001, the WHO hosted a workshop to set research priorities for HSV-2 in LMICs. Periodic re-evaluation of research priorities is essential to ensure effective allocation of resources. This study describes the progress made between 2000 and 2020 in addressing the priorities identified in two of the five thematic areas that were the workshop’s focus: HSV-2 epidemiology and diagnostics. The remaining areas are addressed in a companion paper.MethodsA systematic search of MEDLINE, CINAHL, Global Health and Cochrane databases was carried out. Relevant primary and secondary research studies conducted in LMICs, written in English and published from 2000–2020 were included. Two independent researchers screened, identified papers and extracted preidentified variables from study texts. Data were organised into an Excel spreadsheet and analysed using IBM SPSS V.26.ResultsOverall, 4445 discrete papers were identified, of which 165 publications were eligible for inclusion. The highest general population HSV-2 prevalence was reported in South and West Africa. Prevalence was higher among women than men and increased with age. HSV-2 prevalence studies among key populations were few, and the majority were in East and South Asia. Cohort studies of HSV-2 incidence among younger populations (mean age=25 years) and HSV-2 infection prevalence in North Africa and the Middle East were few. The most researched topic in HSV-2 diagnostics addressed serological techniques and direct molecular biology. Studies of point-of-care testing were also few.ConclusionHSV-2 research identified in LMICs has mainly addressed the epidemiology and diagnostics priorities identified by the 2001 WHO workshop. Unaddressed priorities include point-of-care testing, antiviral resistance and exploration of HSV-2 epidemiology in neglected geographical settings and population subgroups.
Effectiveness of telerehabilitation in patients with post-COVID-19: a systematic review and meta-analysis of randomised controlled trials
Authors: Yang Jiang , Li Huiru , Zhao Hulei , Xie Yang , Li Jiansheng , Wang Minghang 
Published: July 2024

ObjectiveTo assess the effects of telerehabilitation on clinical symptoms, physical function, psychological function and quality of life (QoL) in patients with post-COVID-19.DesignSystematic review and meta-analysis of randomised controlled trials (RCTs).Data sourcesPubMed, Web of Science, Embase and Cochrane Library were searched for publications from 1 January 2020 to 17 April 2024.Eligibility criteriaRCTs investigating the effects of telerehabilitation in patients with post-COVID-19 were included. The outcomes of interest encompassed clinical symptoms, physical function, psychological function and QoL. Only studies reported in English were included.Data extraction and synthesisTwo reviewers independently extracted data and evaluated the risk of bias. Statistical analysis was conducted using Review Manager V.5.3, employing mean difference (MD) with a 95% CI, and the corresponding P value was used to ascertain the treatment effect between groups. Heterogeneity was quantified using theI2statistic. The quality of evidence was assessed by GRADE.Results16 RCTs (n=1129) were included in this systematic review, 15 of which (n=1095, 16 comparisons) were included in the meta-analysis. The primary pooled analysis demonstrated that, compared with no rehabilitation or usual care, telerehabilitation can improve physical function (measured by 30 s sit-to-stand test [6 RCTs, n=310, MD=1.58 stands, 95% CI 0.50 to 2.66; p=0.004]; 6 min walking distance [6 RCTs, n=324, MD=76.90 m, 95% CI 49.47 to 104.33; p<0.00001]; and physical function from the 36-item short-form health survey [5 RCTs, n=380, MD=6.12 units, 95% CI 2.85 to 9.38; p=0.0002]). However, the pooled results did not indicate significant improvements in clinical symptoms, pulmonary function, psychological function or QoL. The quality of the evidence was graded as low for physical function and Hospital Anxiety and Depression Scale-anxiety and very low for other assessed outcomes. The overall treatment completion rate was 78.26%, with no reports of severe adverse events in any included trials.ConclusionsDespite the lack of significant improvements in certain variables, telerehabilitation could be an effective and safe option for enhancing physical function in patients with post-COVID-19. It is advisable to conduct further well-designed trials to continue in-depth exploration of this topic.Study registrationPROSPERO, CRD42023404647.
Comparing the antireflux effect of laparoscopic proximal gastrectomy with double-flap technique reconstruction versus laparoscopic total gastrectomy with Roux-en-Y reconstruction for proximal early gastric cancer: study protocol for a multicentre, prospective, open-label, randomised controlled trial
Authors: Zhou Shengning , Xie Yequan , Zhu Yingying , Tan Jianan , Yang Bin , Zhong Lin , Zhong Guangyu , Han Fanghai 
Published: July 2024

IntroductionLaparoscopic proximal gastrectomy with double flap technique (LPG-DFT) reconstruction has been used for proximal early gastric cancer in recent years. However, its feasibility and safety remain uncertain, as only a few retrospective studies have contained postoperative complications and long-term survival data. LPG-DFT for proximal early gastric cancer is still in the early stages of research. Large-scale, prospective randomised controlled trials (RCTs) are necessary to assess the value of LPG-DFT for proximal early gastric cancer.Methods and analysisThis study is a multicentre, prospective, open-label, RCT that investigates the antireflux effect of LPG-DFT compared with laparoscopic total gastrectomy with Roux-en-Y (LTG-RY) reconstruction for proximal early gastric cancer. A total of 216 eligible patients will be randomly assigned to the LPG-DFT group or the LTG-RY group at a 1:1 ratio using a central, dynamic and stratified block randomisation method, if inclusion criteria are met. General and clinical data will be collected when the patient is enrolled in the study and keep pace with the patient at each stage of his medical and follow-up pathway. The primary endpoint is the proportion of patients with reflux esophagitis (Los Angeles Grade B or more) within 12 months postoperatively. The secondary endpoints included intraoperative outcomes, postoperative recovery, postoperative pain assessment, pathological outcomes, postoperative quality of life, postoperative nutrition status, morbidity and mortality rate, and oncological outcomes (3-year overall survival (OS), 3-year disease-free survival (DFS), 5-year DFS and 5-year OS).Ethics and disseminationThe protocol is approved by the Sun Yat-Sen Memorial Hospital of Sun Yat-Sen University ethics committee (registration number: SYSKY-2022-276-02) on 28 September 2022.We will report the positive as well as negative findings in international peer-reviewed journals.Trial registration numberNCT05890339.
Psychometric of the Persian version of Quality of Life in Late-Stage Dementia (QUALID) in the elderly with Alzheimer’s disease
Authors: Amrollah Majdabadi Kohne Zahra , Ebadi Abbas , Ashghali-Farahani Mansoureh , Tajali Saleheh , Keyvanloo Shahrestanaki Sahar 
Published: July 2024

BackgroundImproving the quality of life (QoL) is a significant healthcare priority, and it is an important health outcome for elderly individuals with Alzheimer’s disease. Quality of Life in Late-Stage Dementia (QUALID) is a specific scale used to measure the QoL in elderly individuals with Alzheimer’s. So far, limited quantitative research has been conducted on the psychometric properties of this scale.AimsThis study was conducted to translate the QUALID Scale into Persian and evaluate its psychometric properties among family and professional caregivers of elderly individuals with Alzheimer’s disease in Tehran.MethodsA cross-sectional methodological study was conducted among family and professional caregivers of elderly individuals with Alzheimer’s in Tehran, Iran in 2022. The questionnaire was translated into Persian using the forward–backward method. Face and content validity were assessed. Additionally, construct validity was examined using exploratory factor analysis (EFA) with Equamax rotation (n=210) and confirmatory factor analysis (CFA) (n=155). Cronbach’s alpha and interclass correlation coefficient (ICC) were estimated to determine reliability.ResultsA total of 365 caregivers with a mean age of 14.18±42.60 years participated in this study. In the face and content validity phase, all 11 items were retained. To determine the construct validity, two factors were extracted in the EFA phase, including behavioural signs of discomfort and behavioural signs of social interaction. The findings of the CFA also indicated that all goodness of fit indices supported the final model. The Cronbach’s alpha was excellent for both factors (0.814), and the ICC was calculated as 0.98.ConclusionBased on the findings of this study, it can be concluded that the Persian version of the QUALID Scale has sufficient validity and reliability for measuring the QoL in elderly Iranian individuals with Alzheimer’s.
Off-the-shelf CAR-NK cells targeting immunogenic cell death marker ERp57 execute robust antitumor activity and have a synergistic effect with ICD inducer oxaliplatin
Authors: Zheng Liuhai , Wang Huifang , Zhou Jihao , Shi Guangwei , Ma Jingbo , Jiang Yuke , Dong Zhiyu , Li Jiexuan , He Yuan-Qiao , Wu Dinglan , Sun Jichao , Xu Chengchao , Li Zhijie , Wang Jigang 
Published: July 2024

BackgroundChimeric antigen receptor natural killer (CAR-NK) therapy holds great promise for treating hematologic tumors, but its efficacy in solid tumors is limited owing to the lack of suitable targets and poor infiltration of engineered NK cells. Here, we explore whether immunogenic cell death (ICD) marker ERp57 translocated from endoplasmic reticulum to cell surface after drug treatment could be used as a target for CAR-NK therapy.MethodsTo target ERp57, a VHH phage display library was used for screening ERp57-targeted nanobodies (Nbs). A candidate Nb with high binding affinity to both human and mouse ERp57 was used for constructing CAR-NK cells. Various in vitro and in vivo studies were performed to assess the antitumor efficacy of the constructed CAR-NK cells.ResultsWe demonstrate that the translocation of ERp57 can not only be induced by low-dose oxaliplatin (OXP) treatment but also is spontaneously expressed on the surface of various types of tumor cell lines. Our results show that G6-CAR-NK92 cells can effectively kill various tumor cell lines in vitro on which ERp57 is induced or intrinsically expressed, and also exhibit potent antitumor effects in cancer cell-derived xenograft and patient-derived xenograft mouse models. Additionally, the antitumor activity of G6-CAR-NK92 cells is synergistically enhanced by the low-dose ICD-inducible drug OXP.ConclusionCollectively, our findings suggest that ERp57 can be leveraged as a new tumor antigen for CAR-NK targeting, and the resultant CAR-NK cells have the potential to be applied as a broad-spectrum immune cell therapy for various cancers by combining with ICD inducer drugs.
Hypoxia inhibits the iMo/cDC2/CD8+ TRMs immune axis in the tumor microenvironment of human esophageal cancer
Authors: Wu Chuanqiang , Yu Huan , Liang Fuxiang , Huang Xiancong , Jiang Bin , Lou Zhiling , Liu Yafei , Wu Zixiang , Wang Qi , Shen Hong , Chen Ming , Wu Pin , Wu Ming 
Published: July 2024

BackgroundEsophageal cancer (ESCA) is a form of malignant tumor associated with chronic inflammation and immune dysregulation. However, the specific immune status and key mechanisms of immune regulation in this disease require further exploration.MethodsTo investigate the features of the human ESCA tumor immune microenvironment and its possible regulation, we performed mass cytometry by time of flight, single-cell RNA sequencing, multicolor fluorescence staining of tissue, and flow cytometry analyses on tumor and paracancerous tissue from treatment-naïve patients.ResultsWe depicted the immune landscape of the ESCA and revealed that CD8+(tissue-resident memory CD8+T cells (CD8+TRMs) were closely related to disease progression. We also revealed the heterogeneity of CD8+TRMs in the ESCA tumor microenvironment (TME), which was associated with their differentiation and function. Moreover, the subset of CD8+TRMs in tumor (called tTRMs) that expressed high levels of granzyme B and immune checkpoints was markedly decreased in the TME of advanced ESCA. We showed that tTRMs are tumor effector cells preactivated in the TME. We then demonstrated that conventional dendritic cells (cDC2s) derived from intermediate monocytes (iMos) are essential for maintaining the proliferation of CD8+TRMs in the TME. Our preliminary study showed that hypoxia can promote the apoptosis of iMos and impede the maturation of cDC2s, which in turn reduces the proliferative capacity of CD8+TRMs, thereby contributing to the progression of cancer.ConclusionsOur study revealed the essential antitumor roles of CD8+TRMs and preliminarily explored the regulation of the iMo/cDC2/CD8+TRM immune axis in the human ESCA TME.
Biological notes of Harpactor angulosus (Lepeletier and Serville, 1825) (Hemiptera: Reduviidae)
Authors: Maza Noelia , Melo María Cecilia 
Published: July 2024

Assassin bugs are one of the most diverse families of cimicomorphan Heteroptera and are known from all biogeographic regions. Except for the kissing bugs (Triatominae) that are hemathophagous, reduviids show a predatory behavior and are usually study as potential biocontrollers of crop pests. In this way, Harpactorines have been specially studied as they are mostly diurnal and live on the vegetation. In this contribution, the complete life cycle of Harpactor angulosus (Lepeletier & Serville, 1825) is described and illustrated. Specimens were collected in the field in El Manantial, San Miguel de Tucumán, Argentina, and were reared in laboratory to obtain all immatures (eggs and nymphs) and to observe its feeding habits, molting, mating, and oviposition. Collection specimens as well as online resources such as iNaturalist records were also studied to know the current distribution of the species. This resulted in the expansion of H. angulosus distribution in Argentina to Tucumán and Buenos Aires provinces and to the recording of the species in Ecuador, Peru and Paraguay for the first time. Our findings reveal that H. angulosus would be studied as a potential biocontroller of Agraulis sp., an important pest of maracuyá crops in several South American countries.
Description of a new alpheid shrimp from the tropical western Atlantic (Decapoda: Caridea: Alpheus)
Authors: Anker Arthur 
Published: July 2024

A new shallow-water species of the alpheid shrimp genus Alpheus Fabricius, 1798 is described based on five specimens from Maceió, Alagoas, northeastern Brazil. Alpheus schubarti sp. nov., belongs to the A. paracrinitus Miers, 1881 species complex and is most closely related to the eastern Pacific A. rostratus Kim & Abele, 1988, from which it can be separated by several morphological differences and the colour pattern of the major and minor chelae.
Imaging of Existing and Newly Translated Proteins Elucidates Mechanisms of Sarcomere Turnover
Authors: Douvdevany Guy , Erlich Itai , Haimovich-Caspi Lilac , Mashiah Tomer , Prondzynski Maksymilian , Pricolo Maria Rosaria , Alegre-Cebollada Jorge , Linke Wolfgang A. , Carrier Lucie , Kehat Izhak 
Published: July 2024

BACKGROUND:
How the sarcomeric complex is continuously turned over in long-living cardiomyocytes is unclear. According to the prevailing model of sarcomere maintenance, sarcomeres are maintained by cytoplasmic soluble protein pools with free recycling between pools and sarcomeres.

METHODS:
We imaged and quantified the turnover of expressed and endogenous sarcomeric proteins, including the giant protein titin, in cardiomyocytes in culture and in vivo, at the single cell and at the single sarcomere level using pulse-chase labeling of Halo-tagged proteins with covalent ligands.

RESULTS:
We disprove the prevailing protein pool model and instead show an ordered mechanism in which only newly translated proteins enter the sarcomeric complex while older ones are removed and degraded. We also show that degradation is independent of protein age and that proteolytic extraction is a rate-limiting step in the turnover. We show that replacement of sarcomeric proteins occurs at a similar rate within cells and across the heart and is slower in adult cells.

CONCLUSIONS:
Our findings establish a unidirectional replacement model for cardiac sarcomeres subunit replacement and identify their turnover principles.
The genus Psyttalia Walker (Hymenoptera, Braconidae) in Vietnam: descriptions of seven new species and their host information
Authors: OANH NGUYEN THI , LONG KHUAT DANG , XUAN TRINH THI , TINH DUONG VAN , NGHIEP HOANG THI 
Published: July 2024

The genus Psyttalia Walker is recorded for the first time from Vietnam with seven species described and fully illustrated as new for science, viz. Psyttalia antenervis Long, sp. nov., P. hoabinhensis Long, sp. nov., P. mangiferae Long & Oanh, sp. nov., P. nigristriata Long, sp. nov., P. quadrinervis Long, sp. nov., P. sonla Long, sp. nov. and P. tricolorata Long, sp. nov. Two species, P. incisi (Silvestri) and P. latinervis Wu & van Achterberg, are newly recorded for Vietnam’s braconid fauna. Additionally, the diagnosis of a putative female of P. latinervis is given. Host records are provided for P. mangiferae, sp. nov., which is mainly reared from tephritid larvae of Oriental fruit fly, Bactrocera dorsalis, infesting fruits of mango, Mangifera indica, in South Vietnam. A key to Vietnamese Psyttalia species is provided, and comparative morphological characters of Psyttalia species are discussed.
Future Changes of Extreme Precipitation and Related Atmospheric Conditions in East Asia under Global Warming Projected in Large Ensemble Climate Prediction Data
Authors: He Sicheng , Takemi Tetsuya 
Published: July 2024

Abstract
Extreme precipitation is expected to pose a more severe threat to human society in the future. This work assessed the historical performance and future changes in extreme precipitation and related atmospheric conditions in a large ensemble climate prediction dataset, the database for Policy Decision-making for Future climate change (d4PDF), over East Asia. Compared with the Tropical Rainfall Measuring Mission (TRMM) and fifth major global reanalysis produced by ECMWF (ERA5) datasets, the historical climate in d4PDF represents favorably the precipitation characteristics and the atmospheric conditions, although some differences are notable in the moisture, vertical motion, and cloud water fields. The future climate projection indicates that both the frequency and intensity of heavy precipitation events over East Asia increase compared with those in the present climate. However, when comparing the atmospheric conditions in the historical and future climates for the same precipitation intensity range, the future climate indicates smaller relative humidity, weaker ascent, less cloud water content, and smaller temperature lapse rate, which negatively affect generating extreme precipitation events. The comparison of the precipitation intensity at the same amount of precipitable water between the historical and future climates indicates that extreme precipitation is weaker in the future because of the more stabilized troposphere in the future. The general increase in extreme precipitation under future climate is primarily due to the enhanced increase in precipitable water in the higher temperature ranges, which counteracts the negative conditions of the stabilized troposphere.

Significance Statement
Extreme precipitation can have disastrous effects on human lives, economy, and ecosystems and is anticipated to significantly increase in both intensity and frequency under future climate. The purpose of this study is to investigate the mechanism for the future change of extreme precipitation. We examined the relationship between future changes in extreme precipitation and changes in the related atmospheric conditions. It is important for reducing uncertainties in future projections of extreme precipitation. Our results highlight that the future atmospheric condition is unfavorable for generating future extreme precipitation events in terms of stability and humidity changes. The increase in the column moisture content is the primary factor for the increase of extreme precipitation, which counteracts the negative conditions.
Examining Outer Band Supercell Environments in Landfalling Tropical Cyclones Using Ground-Based Radar Analyses
Authors: Alford A. Addison , Schenkel Benjamin , Hernandez Samuel , Zhang Jun A. , Biggerstaff Michael I. , Blumenauer Emily , Sandmæl Thea N. , Waugh Sean M. 
Published: July 2024

Abstract
Supercells in landfalling tropical cyclones (TCs) often produce tornadoes within 50 km of the coastline. The prevalence of TC tornadoes near the coast is not explained by the synoptic environments of the TC, suggesting a mesoscale influence is likely. Past case studies point to thermodynamic contrasts between ocean and land or convergence along the coast as a possible mechanism for enhancing supercell mesocyclones and storm intensity. This study augments past work by examining the changes in the hurricane boundary layer over land in the context of vertical wind shear. Using ground-based single- and dual-Doppler radar analyses, we show that the reduction in the boundary layer wind results in an increase in vertical wind shear/storm-relative helicity inland of the coast. We also show that convergence along the coast may be impactful to supercells as they cross the coastal boundary. Finally, we briefly document the changes in mesocyclone vertical vorticity to assess how the environmental changes may impact individual supercells.