bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic effect of long-interval repeated subcutaneous administration of canine amniotic membrane-derived mesenchymal stem cells in atopic dermatitis mouse model
Authors: Kim Minsoo , Kong Dasom , Kim Nam Gyo , Kim Min-Ji , Kim Hee-Yeong , Choi Jung-Ju , Choi Yu-Seung , Lee Ha-Eun , Farzaneh Khaligh Seyedeh , Kwon Dohyung , Lee Seunghee , Kang Kyung-Sun 
Published: February 2025

Abstract
Atopic dermatitis (AD) is a chronic and inflammatory disease. According to a recent study, administration of canine MSCs is a potential therapy for immunological diseases. However, most related studies involve short-term experiments and acute atopic dermatitis animal models. Thus, studies of repeated subcutaneous injection of canine MSCs for ameliorating long-term inflammatory skin disorders have not yet been established. In this study, we evaluated the effects of long-term canine amniotic mesenchymal stem cells (cAM-MSCs) and calcineurin inhibitors (CNIs) treatments in mouse AD model for up to 8 weeks and compared the differences in therapeutic effect through canine peripheral blood mononuclear cells (PBMCs). Using a mouse model, we validated the therapeutic impact of cAM-MSCs in comparison to pimecrolimus (Pime), the most widely used CNIs, as a therapy for canine AD. Based on our results, we verified that the cAM-MSC treatment group exhibited substantially lower scores for tissue pathologic alterations, inflammatory cytokines, and dermatologic symptoms than the PBS control group. Importantly, compared with Pime, cAM-MSCs were more effective at preventing wound dysfunction and regulating mast cell activity. Additionally, we confirmed that immune modulation proteins (TGF-β1, IDO1, and COX-2) were increased in the cAM-MSCs treatment group. Furthermore, we examined the immunoregulatory effect of cAM-MSCs through the proliferation of T lymphocytes from activated canine PBMCs. As a result, cAM-MSCs suppressed the proliferative capacity of effector T cells from canine PBMCs more effectively than Pime. In conclusion, this study suggested that the cAM-MSCS could be an effective canine treatment for long-term canine AD through regeneration and immunomodulation.
Guidelines for the content of statistical analysis plans in clinical trials: protocol for an extension to cluster randomized trials
Authors: Hemming Karla , Thompson Jacqueline Y. , Hooper Richard L. , Ukoumunne Obioha C. , Li Fan , Caille Agnes , Kahan Brennan C. , Leyrat Clemence , Grayling Michael J. , Mohammed Nuredin I. , Thompson Jennifer A. , Giraudeau Bruno , Turner Elizabeth L. , Watson Samuel I. , Goulão Beatriz , Kasza Jessica , Forbes Andrew B. , Copas Andrew J. , Taljaard Monica 
Published: February 2025

Abstract

Background
Guidance exists to inform the content of statistical analysis plans in clinical trials. Though not explicitly stated, this guidance is generally focused on clinical trials in which the randomization units are individual patients and not groups of patients. There are critical considerations for the analysis of cluster randomized trials, such as accounting for clustering, the risk of imbalances between the arms due to post-randomization recruitment, and the need to use small sample corrections when the number of clusters is small.

Methods
This paper outlines the protocol for the development of a set of reporting guidelines for the content of statistical analysis plans for cluster randomized trials (including variations such as the stepped wedge cluster randomized trial and other cluster cross-over designs) by extending the minimum reporting analysis requirements as previously defined for individually randomized trials to cluster randomized trials. The guideline will be developed using a consensus-based approach, modifying existing reporting items from the guideline for individually randomized trials and extending to include new items.

Discussion
The guideline will be developed so it can be used independently of the guideline for individually randomized designs. The consensus guidelines will be published in an open-access journal, including key guidance as well as exploration and elaboration.
Orthodontically induced root resorption in endodontically treated and vital teeth: a cone beam computer tomographic study
Authors: Liu Ziang , Ouyang Yuqing , Lou Yiting , Han Yineng , Lu Mengting , Yu Mengfei , Wang Huiming , Ding Wanghui 
Published: February 2025

Abstract

Background
Orthodontically induced root resorption (OIRR) is a common side effect of orthodontic treatment. This study compares the degree of OIRR between root-filled teeth (RFT) and vital pulp teeth (VPT), and analyzes relevant study variables.

Methods
We conducted a retrospective study on 69 patients who had undergone orthodontic treatment. Using Cone-beam computed tomography (CBCT), we measured changes of root length before and after treatment through a unique method involving three-dimensional (3D) image registration and superimposition. Factors related to the OIRR such as gender, type of treatment, tooth type, age, duration of treatment and distance of root movement were considered.

Results
The sample included 55 females and 14 males aged 27.19 ± 6.08 years. On the basis that there was no significant difference in the root movement distance between RFT and VPT, RFT showed significantly less OIRR than VPT (P < 0.05). Gender did not significantly impact on OIRR for either RFT or VPT group (P > 0.05). In women specifically, RFT displayed less resorption than VPT (P < 0.05). For treatment type, extraction cases demonstrated a lower degree of OIRR in RFT than VPT (P < 0.05), and notable greater OIRR in with-extraction group compared to no-extractions group was found in RFT (P < 0.05), but not in VPT (P > 0.05). Tooth type did not yield significant differences in OIRR overall; however, upper teeth and premolars experienced lower resorption in RFT than in VPT (P < 0.05). Cases treated with fixed appliance had higher OIRR in both RFT and VPT than those with clear aligners (P < 0.05). Age did not correlate significantly with OIRR for either group (P > 0.05). And duration of treatment positively correlated with OIRR for both types (RFT: r = 0.5506, P < 0.0001; VPT: r = 0.4371, P = 0.0002), so did root movement distance (RFT: r = 0.2955, P = 0.0140; VPT: r = 0.2790, P = 0.0206).

Conclusions
RFT exhibit significantly less OIRR than VPT after orthodontic treatment. Treatment type, appliance type, duration of treatment and root movement distance are significant factors influencing OIRR. Personalized orthodontic treatment plans and vigilant monitoring are crucial to mitigate OIRR risks.
A pilot study on the effect of time and acidic/basic conditions on the development of intent fingermarks using silica-copper oxide (SiO2/CuO) nanocomposite
Authors: Grover Aseem , Bumbrah Gurvinder Singh , Das Anirban 
Published: February 2025

Abstract

Background
Fingerprints are the most reliable means of identification. It is widely accepted physical evidence usually found on various items recovered from the crime scenes. It can be used to ascertain the identity of a person or to provide a direct link of suspect to weapon or scene of crime. Various methods have been used to detect the latent fingermarks on porous and nonporous surfaces.

Materials and methodology
In this present study, the novel SiO2/CuO nanocomposites have been synthesized and characterized using the PXRD, FE-SEM, TEM, and FT-IR. First copper oxide nanoparticles were synthesized by precipitation method, and silica nanospheres were separately synthesized by Stober method. Thereafter, as-synthesized copper oxide nanoparticles were coated on the silica spheres. This SiO2/CuO nanocomposites were used to develop fingermarks exposed to various environmental conditions as described in the title.

Results
By using the Debye–Scherrer equation, the average crystalline size of SiO2/CuO nanocomposites was calculated to be 33.68 nm. By SEM analysis, the silica were spherical in shape, and CuO had a rodlike structure. The synthesized nanocomposite develops sharp and clear fingerprints on a wide range of dry, smooth, and nonporous surfaces, i.e., aluminum foil, bakelite sheet, CD back side, CD front side, glass slide, and black ceramic tile which may be discerned in natural light. Further, the synthesized nanocomposite was used to develop latent fingerprints treated with acid and base.

Conclusion
This developed SiO2/CuO nanocomposites are useful in detecting and developing weak and fragmented chance prints from scenes of crime. The synthesized SiO2/CuO nanocomposites are non-hazardous and cost effective.
Long COVID – a critical disruption of cholinergic neurotransmission?
Authors: Leitzke Marco , Roach Donald Troy , Hesse Swen , Schönknecht Peter , Becker Georg-Alexander , Rullmann Michael , Sattler Bernhardt , Sabri Osama 
Published: February 2025

Abstract

Background
Following the COVID-19 pandemic, there are many chronically ill Long COVID (LC) patients with different symptoms of varying degrees of severity. The pathological pathways of LC remain unclear until recently and make identification of path mechanisms and exploration of therapeutic options an urgent challenge. There is an apparent relationship between LC symptoms and impaired cholinergic neurotransmission.

Methods
This paper reviews the current literature on the effects of blocked nicotinic acetylcholine receptors (nAChRs) on the main affected organ and cell systems and contrasts this with the unblocking effects of the alkaloid nicotine. In addition, mechanisms are presented that could explain the previously unexplained phenomenon of post-vaccination syndrome (PVS). The fact that not only SARS-CoV-2 but numerous other viruses can bind to nAChRs is discussed under the assumption that numerous other post-viral diseases and autoimmune diseases (ADs) may also be due to impaired cholinergic transmission. We also present a case report that demonstrates changes in cholinergic transmission, specifically, the availability of α4β2 nAChRs by using (-)-[18F]Flubatine whole-body positron emission tomography (PET) imaging of cholinergic dysfunction in a LC patient along with a significant neurological improvement before and after low-dose transcutaneous nicotine (LDTN) administration. Lastly, a descriptive analysis and evaluation were conducted on the results of a survey involving 231 users of LDTN.

Results
A substantial body of research has emerged that offers a compelling explanation for the phenomenon of LC, suggesting that it can be plausibly explained because of impaired nAChR function in the human body. Following a ten-day course of transcutaneous nicotine administration, no enduring neuropathological manifestations were observed in the patient. This observation was accompanied by a significant increase in the number of free ligand binding sites (LBS) of nAChRs, as determined by (-)-[18F]Flubatine PET imaging. The analysis of the survey shows that the majority of patients (73.5%) report a significant improvement in the symptoms of their LC/MEF/CFS disease as a result of LDTN.

Conclusions
In conclusion, based on current knowledge, LDTN appears to be a promising and safe procedure to relieve LC symptoms with no expected long-term harm.
Re-delimiting oversummering regions of Puccinia striiformis f. sp. tritici in China
Authors: Zhang Jiguang , Li Yuxiang , Liu Di , Zhang Junjie , Zhang Zhibo , Yao Qiang , Xu Xiangming , Hu Xiaoping 
Published: February 2025

Abstract
Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most important diseases on wheat, causing severe yield losses. The pathogen can only survive the summer in the cooler areas of northern and western regions in China. Compared to the main wheat-growing region, the oversummering region is relatively small. Therefore, managing stripe rust in the oversummering region is crucial for controlling the disease nationwide. In this study, we conducted experiments to determine the survival of the predominant Pst races in China under high temperatures and used the data to predict potential Pst oversummering regions. The predominant races (CYR32, CYR33, and CYR34) were able to survive and reproduce at an average temperature of up to 27 °C. Disease incidence and the number of uredinia decreased with increasing temperatures of 24–27 °C during incubation. The results from re-delimiting oversummering regions suggested that Pst is able to oversummer in the east of Gansu province and the south of Shanxi province, which were previously considered unsuitable for Pst oversummering. Based on the present study, the east of Inner Mongolia and north of Heilongjiang could provide the oversummering condition for Pst. Three major oversummering regions, the Liupan Mountain range, the highlands in the south of Gansu province, and the east of Qinghai province were identified. The results should be useful for adjusting strategies to manage stripe rust in China.
Developing an algorithm to identify individuals with psychosis in secondary care in England: application using the Mental Health Services Data Set
Authors: de Oliveira Claire , Matias Maria Ana , Aragon Aragon María José , Anaya Montes Misael , Osborn David , Jacobs Rowena 
Published: February 2025

Background
There is currently no definitive method for identifying individuals with psychosis in secondary care on a population-level using administrative healthcare data from England.

Aims
To develop various algorithms to identify individuals with psychosis in the Mental Health Services Data Set (MHSDS), guided by national estimates of the prevalence of psychosis.

Method
Using a combination of data elements in the MHSDS for financial years 2017–2018 and 2018–2019 (mental health cluster (a way to describe and classify a group of individuals with similar characteristics), Health of the Nation Outcome Scale (HoNOS) scores, reason for referral, primary diagnosis, first-episode psychosis flag, early intervention in psychosis team flag), we developed 12 unique algorithms to detect individuals with psychosis seen in secondary care. The resulting numbers were then compared with national estimates of the prevalence of psychosis to ascertain whether they were reasonable or not.

Results
The 12 algorithms produced 99 204–138 516 and 107 545–134 954 cases of psychosis for financial years 2017–2018 and 2018–2019, respectively, in line with national prevalence estimates. The numbers of cases of psychosis identified by the different algorithms differed according to the type and number (3–6) of data elements used. Most algorithms identified the same core of patients.

Conclusions
The MHSDS can be used to identify individuals with psychosis in secondary care in England. Users can employ several algorithms to do so, depending on the objective of their analysis and their preference regarding the data elements employed. These algorithms could be used for surveillance, research and/or policy purposes.
Precise photopharmacological eradication of metastatic tumor cells
Authors: Varady Adam , Grissenberger Sarah , Wenninger-Weinzierl Andrea , Poplimont Hugo , Sturtzel Caterina , Schmitner Nicole , Gao Li , Kimmel Robin A. , Distel Martin 
Published: February 2025

ABSTRACT
Owing to their high efficacy, antimitotic chemotherapeutics are the mainstay for most cancer treatments. However, these drugs do not discriminate between tumor and healthy cells, thus show dose-limiting toxicity and severe adverse effects. To improve treatments, rendering chemotherapeutics tumor-cell specific is highly desirable. Although various strategies, such as targeted antibody–drug conjugates, aim to achieve this goal, the identification of a tumor-specific ‘Achilles' heel’ remains a challenge. Here, we followed an alternative approach, which does not rely on tumor-specific characteristics, but rather uses spatially confined illumination of the light-activatable microtubule inhibitor SBTubA4P to target its cytotoxic activity to tumor cells. We demonstrate that localized illumination of SBTubA4P allows for precise eradication of disseminated sarcoma cells in zebrafish xenografts without inducing systemic toxicity. In addition to the already-described light-dependent inhibition of microtubule dynamics by SBTubA4P, our data indicate that this molecule creates reactive oxygen species upon UV illumination, which significantly increases its cytotoxic effects. SBTubA4P is a valuable addition to the precision oncology toolbox, and zebrafish xenografts constitute a well-suited model to investigate photoactivatable compounds in vivo.
Strategic Idling in Appointment Systems with Sequential Servers
Authors: Goh You Hui , Li Zihao , Yan Zhenzhen 
Published: February 2025

Problem definition: This paper addresses an appointment scheduling problem involving multiple sequential servers using a distributionally robust optimization (DRO) approach. Two decisions are optimized: the schedule for patient visits and an adjusting policy to rebalance customers’ waiting time across servers. Methodology/results: We formulate the distributionally robust appointment scheduling problem in sequential-servers systems using conic optimization, incorporating service time correlations. We find that the traditional cost-minimization approach results in imbalanced waiting times, concentrated at downstream servers. To address this, we propose strategically idling upstream servers, inspired by queueing literature, and develop a DRO model to jointly optimize the schedule and strategic idling (SI) policy. Through extensive numerical studies, we thoroughly examine the role of SI in a system’s performance and the effect of correlation information on the optimal schedule and SI policy. Finally, using data from a clinic, we conduct a case study to demonstrate the performance advantage of our SI policy, over existing SI policies in the literature. Managerial implications: First, our SI model provides a jointly optimal schedule and SI policy that is effective in balancing waiting times across servers, in some scenarios, and also reduces total waiting time, with the cost borne by the service provider’s overtime. Second, the typical dome-shaped schedule provides an advantage in systems with multiple congested servers, as it evenly distributes congestion across servers. Finally, incorporating correlations lessens the expected cost and improves the patients’ waiting experience with balanced waiting time across servers. Funding: Z. Yan received financial support from the Ministry of Education, Singapore [AcRF Tier 1 Grant RG20/23] and the School of Physical and Mathematical Sciences Collaborative Research Award (2024). Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2022.0278 .
The Stochastic Dynamic Postdisaster Inventory Allocation Problem with Trucks and UAVs
Authors: van Steenbergen R. M. , van Heeswijk W. J. A. , Mes M. R. K. 
Published: February 2025

Humanitarian logistics operations face increasing difficulties due to rising demands for aid in disaster areas. This paper investigates the dynamic allocation of scarce relief supplies across multiple affected districts over time. It introduces a novel stochastic dynamic postdisaster inventory allocation problem (SDPDIAP) with trucks and unmanned aerial vehicles (UAVs) delivering relief goods under uncertain supply and demand. The relevance of this humanitarian logistics problem lies in the importance of considering the intertemporal social impact of deliveries. We achieve this by considering social costs (transportation and deprivation costs) when allocating scarce supplies. Furthermore, we consider the inherent uncertainties of disaster areas and the potential use of cargo UAVs to enhance operational efficiency. This study proposes two anticipatory solution methods based on approximate dynamic programming, specifically decomposed linear value function approximation (DL-VFA) and neural network value function approximation (NN-VFA) to effectively manage uncertainties in the dynamic allocation process. We compare DL-VFA and NN-VFA with various state-of-the-art methods (e.g., exact reoptimization and proximal policy optimization) and results show a 6%–8% improvement compared with the best benchmarks. NN-VFA provides the best performance and captures nonlinearities in the problem, whereas DL-VFA shows excellent scalability against a minor performance loss. From a practical standpoint, the experiments reveal that consideration of social costs results in improved allocation of scarce supplies both across affected districts and over time. Finally, results show that deploying UAVs can play a crucial role in the allocation of relief goods, especially in the first stages after a disaster. The use of UAVs reduces transportation and deprivation costs together by 16%–20% and reduces maximum deprivation times by 19%–40% while maintaining similar levels of demand coverage, showcasing efficient and effective operations. History: This paper has been accepted for the Transportation Science Special Issue on TSL Conference 2023.
Formulation and sensory characterization of andean bread enriched with olluco flour (ullucus tuberosus)
Authors: Cárdenas-Jarama Martín , Bohórquez-Medina Andrea L. , Bohórquez-Medina Sofía L. , Cardenas Agurto Carlos , Guillen Rojas Karen 
Published: February 2025

Introduction: Bread is a staple food that provides carbohydrates, proteins, and essential micronutrients. Recently, there has been an increased interest in alternative flours, such as those from tubers, due to their health benefits, such as higher fiber and resistant starch content, which promote satiety and improve glycemic control. Methodology: This study evaluated the sensory acceptability of olluco flour, a Peruvian tuber, in bread making. Three samples were prepared with 30%, 50%, and 70% olluco flour, assessed by a panel of ten experts using a 9-point Likert scale and a 10-point star diagram. For the analysis of the sensory evaluation data, the spps and excel programs were used. Results: The sensory analysis showed that the inclusion of olluco flour is viable, but concentrations above 50% negatively affected overall acceptability. The bread with 30% olluco flour achieved the best balance between nutritional value and sensory quality. Conclusion: The incorporation of olluco flour in bread is a promising strategy to enrich baked products with nutritious ingredients. The properties of olluco also allow for its application in other recipes, reducing calories and improving texture. Future studies should explore optimal formulations and broader applications.
Eavesdropping on bats in Peninsular Thailand: a trial application of automated recorders to monitor habitat changes
Authors: Yu Grace Rui-Tong , Dejtaradol Ariya , Karapan Sunate , Soisook Pipat , Lee Chia-Yun , Tuanmu Mao-Ning , Yu Hon-Tsen 
Published: February 2025

We deployed automated bat recorders for one week in the southern part of Peninsular Thailand in an attempt to monitor bat species diversity and activities. Two sites were chosen: one on a forested slope adjacent to urban development, the campus of Prince of Songkla University (PSU), and the other in a natural tropical rainforest, the Hala-Bala Wildlife Sanctuary (Bala). From PSU, we analyzed 9,744 5s time windows of recordings that were obtained from the dry season (June 2023) and the wet season (October 2023); from Bala, we analyzed 4,692 5s time windows in the wet season (October 2023). Among a total of 14,436 time windows, we detected bat acoustic signals in 1986 (13.8 %) representing 10 species of bat: eight species at PSU and six species at Bala. The recordings permitted analyses of diel activity patterns for the four species with the most acoustic records, as well as estimates of relative species abundance in accordance with forest type and season. Our results demonstrate that using automated bat recorders can help unravel bat diversity, activity patterns, and the potential for interspecific interactions. Nonetheless, independent efforts to collect and verify acoustic signals by catching and observing live bats are needed to ensure accurate species identification.