bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population structure and genetic diversity of mango (Mangifera indica L.) germplasm resources as revealed by single-nucleotide polymorphism markers
Authors: Liang Qingzhi , Pan Hongbing , He Xiaolong , Wang Songbiao , Hou Yuanhua , Xiao Hua , Xu Guangzhao , Yi Runhua , Lin Dongbo , Yang Zhuanying 
Published: July 2024

IntroductionMango is a vital horticultural fruit crop, and breeding is an essential strategy to enhance ongoing sustainability. Knowledge regarding population structure and genetic diversity in mango germplasm is essential for crop improvement.MethodsA set of 284 mango accessions from different regions of the world were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction to generate genomic single-nucleotide polymorphism (SNP).ResultsAfter filtering, raw data containing 539.61 M reads were obtained. A total of 505,300 SLAFs were detected, of which, 205,299 were polymorphic. Finally, 29,136 SNPs were employed to dissect the population structure, genetic relationships, and genetic diversity. The 284 mango accessions were divided into two major groups: one group consisted mainly of mango accessions from Australia, the United States, Cuba, India, Caribbean, Israel, Pakistan, Guinea, Burma, China, and Sri Lanka, which belonged to the Indian type (P1); the other group contained mango accessions from the Philippines, Thailand, Indonesia, Vietnam, Cambodia, Malaysia, and Singapore, which belonged to Southeast Asian type (P2). Genetic diversity, principal component analysis (PCA), and population structure analyses revealed distinct accession clusters. Current results indicated that the proposed hybridization occurred widely between P1 and P2.DiscussionMost of the accessions (80.99%) were of mixed ancestry, perhaps including multiple hybridization events and regional selection, which merits further investigation.
Consequential life cycle assessment of demolition waste management in Germany
Authors: Dierks Christian , Hagedorn Tabea , Mack Theresa , Zeller Vanessa 
Published: July 2024

ContextBulk mineral waste materials such as construction and demolition waste are Germany’s largest waste stream. Despite the availability of high-quality recycling pathways such as road base layers, waste concrete is predominantly recycled into lower-quality recycling pathways like earthworks or unbound road construction. This is due to low demand for recycled aggregates in road base layers and frost protection layers, especially in public procurement.PurposeThis study assesses the environmental consequences of increasing high-quality recycling of waste concrete in the near future to provide decision support for public procurement in Germany. The focus lies on climate change due to its importance for decision-makers. However, 17 other impact categories were assessed to avoid problem shifting.MethodsLife cycle assessment (LCA) is applied with background data from ecoinvent 3.9.1. Impact assessment was conducted at midpoint level using IPCC 2021 and ReCiPe Midpoint (H). Foreground data were taken from literature and expert interviews. In line with the goal of this LCA, a consequential modeling approach was followed to account for changes in the material flow system. Substitution creates a cascade effect previously omitted in consequential LCA studies, in which lower quality recycling materials replace higher quality recycling materials in their respective utilization pathways.Results and discussionIncreasing the high-quality recycling of waste concrete into road base layers causes a reduction in environmental impacts for all 18 impact categories, as it replaces natural aggregate and avoids backfilling of mixed mineral waste and excavated earth through substitution effects. Transport distances and ferrous metal recovery were identified as hot spots. Sensitivity analyses show that only transport is a significant issue.ConclusionIncreasing the high-quality recycling of waste concrete in Germany is recommended in terms of environmental impacts. Lower-quality recycling is environmentally feasible only in cases where the avoided transport distances for natural aggregates and backfilling are significantly lower than the additional transport distances for high-quality recycling.
Intermediate E-F layer dynamics study in the Brazilian low-latitude sector: observational data and simulations
Authors: Muella Marcio T. A. H. , Silva Ana P. M. , Santos Ângela M. dos , Pillat Valdir G. , Resende Laysa C. A. , Andrioli Vânia F. , Fagundes Paulo R. 
Published: July 2024

This study investigates the downward motion of Intermediate E-F Layers (ILs) in the Brazilian low latitude sector through observation and modeling. Ionosonde data from São José dos Campos (SJC) and Palmas (PAL) were analyzed to investigate the seasonal variation of the IL parameters, including the virtual height (h'IL) and the top frequency (ftIL). The ILs primarily originated from F layer detachment followed by downward motion, peaking before 11 LT and disappearing well before sunset. Daily height variability ranged between 130 and 190 km, with peak frequencies around 4–5 MHz. Using meteor radar data as input, the Ionospheric E-region Model (MIRE) simulated diurnal and semidiurnal tides to analyze neutral wind effects on ILs descent. Model simulations for SJC (October 2008) and PAL (April and June 2009) revealed distinct wind oscillations influencing IL dynamics at heights below 140 km. In SJC, meridional wind shears controlled IL descent, with possible zonal wind interactions weakening ILs. Conversely, in PAL during April 2009, both zonal and meridional winds contributed to IL formation and altitude descent. However, discrepancies between observed and modeled descent rates suggest the need for considering additional atmospheric wave interactions in future modeling studies. June 2009 over PAL presented unique IL behavior, exhibiting a lower observed decay rate and daily height oscillations potentially linked to local modulations. Meanwhile, MIRE indicated that meridional wind shearing predominantly controlled IL descent in the morning, with zonal wind becoming relevant post-midday. These findings enhance our understanding of IL dynamics and their atmospheric drivers.
Susceptibility evaluation and PK/PD integration of tulathromycin against Actinobacillus pleuropneumoniae during the mutant selection window
Authors: Wang Hongjuan , Zhang Longfei 
Published: July 2024

IntroductionActinobacillus pleuropneumoniae (APP) is a serious pathogen that affects the development of livestock breeding. Due to excessive use of antimicrobial drugs, many multidrug-resistant bacteria have emerged and spread, which have threatened the livestock industry. Therefore, we established a peristaltic pump infection model (PPIM) to evaluate the susceptibility change and pharmacokinetic/pharmacodynamic (PK/PD) integration of tulathromycin against APP during the mutant selection window (MSW) for preventing the emergence of mutant-resistant bacteria.MethodsThe 99% minimum inhibitory concentration (MIC99) and mutant prevention concentration (MPC) of tulathromycin against APP were measured using the agar-plate method. After the model of dynamic infection had been established based on tulathromycin data in lungs, different dosages were administered to make the drug concentrations located in different parts of the MSW. The population and sensitivity of APP were monitored. Tulathromycin concentrations were measured by high-performance liquid chromatography-tandem mass spectrometry. Finally, a sigmoid Emax model was used to analyze the relationships between PK/PD parameters and antibacterial effects.Results and discussionThe values of MIC, MIC99, and MPC of tulathromycin against APP were 2, 1.4, and 44.8 μg/mL, respectively. The PPIM was stable. An elimination effect without regrowth was observed at 5.6 to 44.8 μg/mL (−4.48 to −7.05 Log10 CFU/mL, respectively). The MIC of APP increased 32-fold at 8 MIC99. AUC168 h/MIC99 had the best fit with the antibacterial effect (R2 = 0.9867). The AUC168 h/MIC99 required to achieve bacteriostatic, bactericidal, and clearance effects were 1.80, 87.42, and 198 h, respectively. Our results could provide guidance for the clinical application of tulathromycin to treat APP infection and avoid the generation of drug-resistant bacteria.
Leisure-Time Physical Activity Participation in Middle-Aged and Older Adults With a Spinal Cord Injury in Australia
Authors: Stendell Laura , Stubbs Peter W. , Rogers Kris , Verhagen Arianne P. , Middleton James W. , Davis Glen M. , Arora Mohit , Marshall Ruth , Geraghty Timothy , Nunn Andrew , Quel de Oliveira Camila 
Published: July 2024

ObjectivesAdults with spinal cord injury (SCI) are often sedentary, increasing their risk of cardiometabolic diseases. Leisure-time Physical Activity (LTPA) is physical activity completed during recreation time for enjoyment. We aimed to quantify LTPA in people ≥45 years with SCI and to explore its relationship with participants’ characteristics.MethodsThis is a secondary analysis on a subset of the Australian International SCI Survey in participants ≥45 years, at least 12 months post-injury. We described levels of LTPA and used multivariable regressions to estimate the associations between participant characteristics and LTPA.ResultsOf 1,281 participants (mean age: 62.7 years, mean time since injury: 18.7 years; 74% males) 44% reported no participation in LTPA. The average LTPA participation was 197 (SD 352) minutes per week (median: 50). Females (β = −62.3, 95% CI [−112.9, −11.7]), and participants with non-traumatic injuries (β = −105.2, 95% CI [−165.9, −44.6]) performed less LTPA. Time since injury was not associated with moderate-to-heavy LTPA (LR: Probability &gt; F = 0.785).ConclusionLTPA promotion in the SCI population ≥45 years focusing on females and non-traumatic injuries is warranted.
Comparison of Immune Indicators Related to Phagocytosis of Five Species of Sea Urchins under Artificial Infection with the Pathogenic Bacterium of Black Mouth Disease
Authors: Tian Wenzhuo , Wang Zhong , Leng Xiaofei , Liu Peng , Guo Hao , Jiang Xuechun , Ou Fanjiang , Jia Tongshan , Ding Jun , Zhang Weijie , Chang Yaqing 
Published: July 2024

To screen for immune indicators closely related to disease resistance, two species of sea urchin susceptible to black mouth disease (Strongylocentrotus intermedius, S. intermedius ♀ × Heliocidaris crassispina ♂) and three species of sea urchin resistant to black mouth disease (H. crassispina, H. crassispina ♀ × S. intermedius ♂ and Mesocentrotus nudus) were artificially infected with the black mouth pathogen Vibrio echinoideorum. The phagocytosis-related immune indices of the five sea urchin species were compared at different time points post-infection. The results demonstrated that the parameters such as apoptotic rate of phagocytes, mean contribution value (MCV) of single effective phagocyte on Acid Phosphatase (ACP), Reactive Oxygen Species (ROS), and Total Antioxidant Capacity (T-AOC) of the five sea urchin species first increased and then decreased after infection. The key time points were 3 h to 6 h and 48 h post-infection when the black mouth disease-resistant and susceptible sea urchins demonstrated differences. At 3 h or 6 h post-infection, the up-regulation folds in MCV of ACP, ROS and T-AOC of black mouth disease-resistant sea urchins were considerably higher than that of the susceptible sea urchins. At 6 h post-infection, the apoptosis rate and the phagocytic index (PI) of the black mouth disease-resistant sea urchins were significantly higher than those of the susceptible sea urchins (p < 0.05). At 48 h post-infection, the necrosis rate of phagocytes, MCV of ACP and MCV of ROS of the black mouth disease-resistant sea urchins were significantly lower than those of the susceptible sea urchins (p < 0.05). The apoptosis and necrosis rate of phagocytes, PI, and MCV on ACP, ROS may be used as indicators of disease resistance in sea urchins. Disease resistance standards in immune indices can be summarized as phagocytosis increases greatly in the early infection stage and decreases timely to a normal level after killing the pathogen in a short period.
Unveiling the Nexus: Sulphur Dioxide Exposure, Proximity to Mining, and Respiratory Illnesses in Kankoyo: A Mixed-Methods Investigation
Authors: Chihana Sipiwe , Mbale Jameson , Chaamwe Nchimunya 
Published: July 2024

The emission of sulphur dioxide (SO 2) from mining activities presents significant health hazards, particularly to communities near industrial zones. This mixed-methods study investigates the nexus between (SO 2) exposure and respiratory health in Kankoyo Township, Zambia. Employing community engagement, expert interviews, spatial analysis, and a retrospective examination of 15 years of health and (SO 2) data, the research identified a troubling correlation between (SO 2) exposure and adverse respiratory health effects among the local population. Expert interviews highlighted that respiratory issues constituted approximately 75% of health complications, with a notable reduction in asthma cases following the installation of a monitoring station and upgrades to smelter operations. Spatial analysis demonstrated that (SO 2) levels in Kankoyo exceeded the Zambian Environmental Management Agency (ZEMA) limits by 1713% identifying it as a significant pollution hotspot. Additionally, wind profile analysis indicated frequent low-speed winds from the east-northeast (ENE), contributing to pollutant accumulation. Based on these insights, the study recommends implementing real-time pollution data sharing, affordable air quality sensors, addressing medication shortages, establishing specialized respiratory clinics, launching IT-driven awareness campaigns, and further research into additional pollutants and confounding factors.
In Vitro Effects of Postmetabolites from Limosilactobacillus fermentum 53 on the Survival and Proliferation of HT-29 Cells
Authors: Moskova-Doumanova Veselina , Vaseva Anita , Veleva Ralitsa , Mladenova Kirilka , Melniska Denitsa , Doumanov Jordan , Videv Pavel , Topouzova-Hristova Tanya , Dobreva Lili , Atanasova Nikoleta , Danova Svetla 
Published: July 2024

Naturally fermented dairy products are an important component of the human diet. They are a valuable source of nutrients as well as vitamins and minerals. Their importance as a source of probiotic bacterial strains should not be overlooked. A number of studies highlight the positive effects of species of the probiotic lactic acid bacteria on the intestinal microbiome and the overall homeostasis of the body, as well as a complementary treatment for some diseases. However, data on the effects on the intestinal epithelial cells of postmetabolites released by probiotic bacteria are incomplete. This is likely due to the fact that these effects are species- and strain-specific. In the present study, we investigated the effects of postmetabolites produced by a pre-selected candidate probiotic strain Limosilactobacillus fermentum on HT-29 intestinal epithelial cells. Our data showed a pronounced proliferative effect, evaluated by flow cytometry, quantification of the cell population and determination of the mitotic index. This was accompanied by the stabilization of the cell monolayer, measured by an increase in TEER (transepithelial electric resistance) and the reorganization of actin filaments. The data obtained are a clear indication of the positive effects that the products secreted by L. fermentum strain 53 have on intestinal epithelial cells.
Selective Antineoplastic Potential of Fractionated Caribbean Native Ganoderma Species Extracts on Triple-Negative Breast Cancer Cells
Authors: Arroyo-Cruz Luz V. , Sagardía-González Sebastián , Miller Kurt , Ling Taotao , Rivas Fatima , Martínez-Montemayor Michelle M. 
Published: July 2024

Triple-negative breast cancer (TNBC) is an aggressive subtype characterized by the absence of estrogen receptor, progesterone receptor, and human epidermal growth factor receptor type 2 expression. It is known for its high malignancy, invasiveness, and propensity for metastasis, resulting in a poor prognosis due to the absence of beneficial therapeutic targets. Natural products derived from mushrooms have gained significant attention in neoplastic therapy due to their potential medicinal properties. The therapeutic potential of Ganoderma lucidum in breast cancer has been highlighted by our group, suggesting its use as an adjuvant treatment. The present study aims to assess the potential antineoplastic capacity of two Caribbean native Ganoderma species found in Puerto Rico, Ganoderma multiplicatum (G. multiplicatum) and Ganoderma martinicense (G. martinicense). Antiproliferative studies were conducted via cell viability assays after cultivation, harvesting, and fractionation of both species. The obtained results indicate that most of the fractions show some cytotoxicity against all cell lines, but 33% of the fractions (F1, F2, F7, F12) display selectivity towards cancer cell models. We demonstrate for the first time that native Ganoderma species can generate metabolites with anti-TNBC properties. Future avenues will focus on structure elucidation of the most active fractions of these Ganoderma extracts.
THE EFFECT OF PRE-CONCEPTIONAL NUTRITION EDUCATION ON INCREASING KNOWLEDGE IN PRE-MARRIAGE WOMEN IN GARUT DISTRICT
Authors: Sandy Aulia Putri , Resmana Rika , Noviani Fadilah Lola 
Published: July 2024

Introduction: Many nutritional problems are associated with high maternal and child morbidity rates. Maternal health status is a determinant of the quality of future generations, so it is important to improve the nutritional quality of women early on. Pre-marital women are a priority for health improvement because they are in the pre-conception period. This is a crucial period because it will determine the quality of the pregnancy and the welfare of the baby being born. Knowledge as a factor that determines concern for the fulfillment of balanced nutrition. Special efforts through preconception nutrition education for brides and grooms need to be made in order to achieve a healthy family and quality offspring. The purpose of this study was to determine whether there was an increase in knowledge through pre-conceptional nutrition education for women of premarital childbearing age in Garut district.
Objectives: The number of respondents in this study were 30 people selected by purposive sampling method. The data analysis used is the T-dependent statistical test because the data is normally distributed.
Method: This study used a pre-experimental design with one group pre-posttest design. The population in this study were women of childbearing age who had planned a wedding in Garut district.
Result: The results showed that preconception nutrition education had a significant effect (p value = 0.000) on knowledge with an average increase of 1.77.
Conclusion: It is recommended that the health office and the ministry of religion organize nutrition education activities for premarital women in preparing for a healthy pregnancy.
A correlação entre atividade física e o uso de substâncias psicoativas em estudantes de medicina
Authors: Silva Igor Fabiano da , Boleta Daniela de Cassia Faglioni , Novais Emerson Franco de , Móvio Luiz Fernando Craus , Araújo Luana Sula Sousa dos Reis , Colombo Eliane Mendes Franco , Diegues Maria Elena Martins 
Published: July 2024

Objetivo: Verificar a relação do uso de substâncias psicoativas e a prática de atividade física em estudantes de medicina em uma instituição privada de ensino superior do Paraná. Método: A pesquisa adota uma abordagem quantitativa e descritiva com um corte transversal analítico. Foram aplicados questionários via Google Forms a todos os estudantes de medicina da universidade entre março e junho de 2023. Resultado: Participaram 383 estudantes dos quais 82,6% (n = 315) faziam uso de substâncias psicoativas. Dentre os participantes, 74,5% (n = 284) praticavam atividade física. Foi encontrada uma relação entre a prática de atividade física e menores níveis de estresse, maior qualidade de sono e menores taxas de ansiedade em seu cotidiano. Conclusão: Não foi mensurada uma relação direta entre a atividade física e redução no uso de substâncias. No entanto, destacam-se os benefícios do exercício físico nos demais segmentos da vida, que influenciam significativamente no menor uso.
Rastreio de casos de Esquistossomose na última década em Minas Gerais/Brasil
Authors: Prata Thalles Henrique , Diniz Mariana de Faria Gardingo , Barbosa Filipe Alves Costa , Aguiar Thiago Peixoto 
Published: July 2024

A esquistossomose mansoni, comumente conhecida como "doença do caramujo" e/ou "barriga d'água", é uma doença parasitária causada pelo trematódeo Schistosoma mansoni, que tem caramujos do gênero Biomphalaria como hospedeiros intermediários e humanos como hospedeiros definitivos. O objetivo do presente estudo é coletar dados a respeito da notificação de Esquistossomose nos últimos 10 anos no estado de Minas Gerais, correlacionando com a importância da prevenção, diagnóstico precoce e tratamento. Este estudo é de caráter descritivo, em que os dados avaliados são referentes a usuários do sistema de saúde no estado de Minas Gerais - Brasil, através do DATASUS, selecionando as notificações sobre a Esquistossomose, entre o período de 2014 a 2023. Nos resultados, evidenciou-se na tabela 1, que Minas Gerais apresenta um número de casos de Esquistossomose extremamente elevado, visto que na última década obteve-se 20.567 registros, número esse considerado alarmante. Conclui-se que Minas Gerais é um estado endêmico para a Esquistossomose, havendo elevados registros de casos mediante coleta dos dados no Sinan, entre 2014 e 2023, corroborando-se com a literatura.
As principais complicações da Sífilis Congênita: uma revisão de bibliografia
Authors: Oliveira Isabella Eduarda de Godoy , França Millena Matheus , Montenegro Luisa Rasia , Cruz Isabela Chein Andere , Dios Camila Franzotti , Cardoso Maria Luiza Santos , Maia Lhanne Hanne Duarte , Loesch Maria Clara Ribeiro , Cavalheiro Maria Clara Guedes 
Published: July 2024

A sífilis congênita ocorre após infecção transplacentária, cujo agente é o Treponema pallidum. Dessa forma, as consequências da ausência do tratamento do recém-nascido podem acarretar sequelas irreversíveis, como surdez, cegueira e retardo mental. Entretanto, a sífilis congênita é uma questão de saúde pública e necessita ser prevenida, tratada e diagnosticada de forma precoce, a fim de amenizar os riscos à mãe e ao recém nascido. Contudo, o presente estudo teve por objetivo esclarecer acerca das possíveis complicações da sífilis congênita. Dessa forma, foi realizada uma busca nas principais bases de dados e através da seleção criteriosa realizada, selecionou-se 13 artigos para a elaboração do estudo. Por fim, concluiu-se que a sífilis corrobora com diversas complicações maternas e neonatais, dentre elas restrimento fetal, aborto, partos prematuros e complicações envolvendo o sistema nervoso central. Assim, é de suma importância que as autoridades sanitárias tratem tal temática como questão de saúde pública .