bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hdac3 Deficiency Increases Marrow Adiposity and Induces Lipid Storage and Glucocorticoid Metabolism in Osteochondroprogenitor Cells
Authors: McGee-Lawrence Meghan E , Carpio Lomeli R , Schulze Ryan J , Pierce Jessica L , McNiven Mark A , Farr Joshua N , Khosla Sundeep , Oursler Merry Jo , Westendorf Jennifer J 
Published: July 2015

ABSTRACT

Bone loss and increased marrow adiposity are hallmarks of aging skeletons. Conditional deletion of histone deacetylase 3 (Hdac3) in murine osteochondroprogenitor cells causes osteopenia and increases marrow adiposity, even in young animals, but the origins of the increased adiposity are unclear. To explore this, bone marrow stromal cells (BMSCs) from Hdac3-depleted and control mice were cultured in osteogenic medium. Hdac3-deficient cultures accumulated lipid droplets in greater abundance than control cultures and expressed high levels of genes related to lipid storage (Fsp27/Cidec, Plin1) and glucocorticoid metabolism (Hsd11b1) despite normal levels of Pparγ2. Approximately 5% of the lipid containing cells in the wild-type cultures expressed the master osteoblast transcription factor Runx2, but this population was threefold greater in the Hdac3-depleted cultures. Adenoviral expression of Hdac3 restored normal gene expression, indicating that Hdac3 controls glucocorticoid activation and lipid storage within osteoblast lineage cells. HDAC3 expression was reduced in bone cells from postmenopausal as compared to young women, and in osteoblasts from aged as compared to younger mice. Moreover, phosphorylation of S424 in Hdac3, a posttranslational mark necessary for deacetylase activity, was suppressed in osseous cells from old mice. Thus, concurrent declines in transcription and phosphorylation combine to suppress Hdac3 activity in aging bone, and reduced Hdac3 activity in osteochondroprogenitor cells contributes to increased marrow adiposity associated with aging. © 2015 American Society for Bone and Mineral Research.
Efficacy and safety of therapeutic plasma exchange by using apheresis devices in pediatric atypical hemolytic uremic syndrome patients
Authors: Hans Rekha , Sharma Ratti Ram , Marwaha Neelam , Suri Deepti , Kumar Rakesh , Gupta Anju , Singh Surjit 
Published: July 2015

Background:Therapeutic plasma exchange (TPE) in atypical hemolytic uremic syndrome (aHUS) is considered as first line treatment as per current American Society for Apheresis (ASFA) guidelines. But there is very limited data available in the literature regarding efficacy and safety of TPE procedures in pediatric aHUS patients.Aim:To assess the safety and efficacy of TPE by using apheresis devices in pediatric aHUS patients.Materials and Methods:We did a retrospective analysis of all TPE procedures performed in aHUS pediatric patients over a period of 13 years (2001–2013). TPE procedures were done on two different devices daily or on alternate days depending on clinical condition of the patient. Adverse events if any were noted and analyzed. Pre and post procedural laboratory profiles were analyzed to assess the response to TPE therapy and patients were categorized accordingly.Results:A total of 169 TPE procedures (range of 1–22/patient with an average of 7.6 procedures/patient) were performed on 30 pediatric patients. Twenty four patients had more than 3 TPE procedures. Sixteen patients were complete responders, 5 were partial responders and 3 were non responders. The time between onset of illness and start of TPE therapy was 1–4 days in complete responders, 5–7 days in partial responders and 8–9 days in non‐ responders. Adverse events were observed in 13 (7.7%) procedures.Conclusion:TPE is a safe and effective therapeutic modality in pediatric aHUS if instituted early in the course of disease with a minimum of four to five procedures. J. Clin. Apheresis 31:381–387, 2016. © 2015 Wiley Periodicals, Inc.
The influence of MicroRNA‐150 in Osteoblast Matrix Mineralization
Authors: Dong Chun‐Ling , Liu Hao‐Zhi , Zhang Zhen‐Chun , Zhao Huan‐Li , Zhao Hui , Huang Yan , Yao Jian‐Hua , Sun Tian‐Sheng 
Published: July 2015

ABSTRACTThis study investigated the influence of miR‐150 expression on osteoblast matrix mineralization and its mechanisms. The mouse osteoblast cell line MC3T3‐E1 was used as an in vitro model of bone formation. On the fifth day of mineralization, transfection experiments using agomiR‐150, agomiR‐NC, antagomiR‐150 antagomiR‐NC, and mock groups were set up to test the effects of miR‐150 in MC3T3‐E1 model. The mRNA and protein levels of OC, ALP, type I collagen, and OPN were measured by qRT‐PCR and ELISA. Matrix mineralization was detected by alizarin red S (ARS) staining and flow cytometry was employed to quantify apoptosis in each group. RT‐PCR and Western blot were applied to detect the expression of target gene MMP14. Our results demonstrated that the endogenous expression levels of miR‐150, OC, ALP, type I collagen, and OPN in MC3T3‐E1 cells increased steadily. Exogenous expressions of agomiR‐150 and antagomiR‐150 can significantly up‐/down‐regulate, respectively, the expression level of miR‐150 in MC3T3‐E1 cells. Compared with the mock group, higher expression levels of OC, ALP, type I collagen, and OPN mRNA were observed in the agomiR‐150 group, while lower mRNA expression levels of OC, ALP, type I collagen, and OPN were found in the antagomiR‐150 group. Based on these results, potential miR‐150 targeted genes are discussed. Our results showed that miR‐150 supports the osteoblastic phenotype related to osteoblast function and bone mineralization. Thus, miR‐150 may have potential therapeutic applications in promoting bone formation in certain disease settings, such as in osteoporosis and in elderly patients. J. Cell. Biochem. 116: 2970–2979, 2015. © 2015 Wiley Periodicals, Inc.
TRAIL‐Induced Caspase Activation Is a Prerequisite for Activation of the Endoplasmic Reticulum Stress‐Induced Signal Transduction Pathways
Authors: Lee Dae‐Hee , Sung Ki Sa , Guo Zong Sheng , Kwon William Taehyung , Bartlett David L. , Oh Sang Cheul , Kwon Yong Tae , Lee Yong J. 
Published: July 2015

ABSTRACTIt is well known that tumor necrosis factor‐related apoptosis‐inducing ligand (TRAIL)‐induced apoptosis can be initially triggered by surface death receptors (the extrinsic pathway) and subsequently amplified through mitochondrial dysfunction (the intrinsic pathway). However, little is known about signaling pathways activated by the TRAIL‐induced endoplasmic reticulum (ER) stress response. In this study, we report that TRAIL‐induced apoptosis is associated with the endoplasmic reticulum (ER) stress response. Human colorectal carcinoma HCT116 cells were treated with TRAIL and the ER stress‐induced signal transduction pathway was investigated. During TRAIL treatment, expression of ER stress marker genes, in particular the BiP (binding immunoglobulin protein) gene, was increased and activation of the PERK (PKR‐like ER kinase)‐eIF2α (eukaryotic initiation factor 2α)‐ATF4 (activating transcription factor 4)‐CHOP (CCAAT‐enhancer‐binding protein homologous protein) apoptotic signal transduction pathway occurred. Experimental data from use of a siRNA (small interfering RNA) technique, caspase inhibitor, and caspase‐3‐deficient cell line revealed that TRAIL‐induced caspase activation is a prerequisite for the TRAIL‐induced ER stress response. TRAIL‐induced ER stress was triggered by caspase‐8‐mediated cleavage of BAP31 (B cell receptor‐associated protein 31). The involvement of the proapoptotic PERK‐CHOP pathway in TRAIL‐induced apoptosis was verified by using a PERK knockout (PERK−/−) mouse embryo fibroblast (MEF) cell line and a CHOP−/− MEF cell line. These results suggest that TRAIL‐induced the activation of ER stress response plays a role in TRAIL‐induced apoptotic death. J. Cell. Biochem. 117: 1078–1091, 2016. © 2015 Wiley Periodicals, Inc.
Magnetic mesoporous enzyme–silica composites with high activity and enhanced stability
Authors: Cui Jiandong , Feng Yuxiao , Yue Shu , Zhao Yamin , Li Linbo , Liu Ronglin , Lin Tao 
Published: July 2015

AbstractBACKGROUNDEncapsulation of enzymes in biomimetic silica seems to be a promising immobilization method due to mild processing conditions and short preparation time. However, biomimetic silica particles may be considered too small for industrial applications because they result in difficult recovery. Furthermore, the use of R5 peptide or polymers as catalyst usually causes protein denaturation. To overcome these drawbacks, a new stable and recyclable biocatalyst based on co‐encapsulation of magnetic nanoparticles and phenylalanine ammonia lyase (PAL) in biomimetic silica has been prepared using enzyme‐assisted direct condensation reactions of silicon oxide with tetramethoxysilane (TMOS).RESULTSUnder optimal conditions (10 mg mL−1 nanoparticles, 4.5 U mL−1 PAL, 0.8 mol L−1 TMOS), the highest activity recovery (52%) of encapsulated PAL was obtained. Compared with conventional PAL encapsulated in biomimetic silica (28%), the activity recovery was increased 1.86‐fold. TEM, CLSM, magnetisation measurements, and FTIR confirmed that the magnetic nanopaticles and PAL had been co‐encapsulated into the biomimetic silica. The resulting immobilized PAL exhibited higher activity and stability than native PAL, and the immobilized PAL can be easily separated by applying a magnetic field.CONCLUSIONSThis work provides a convenient strategy to prepare stable and easy recycled biocatalyst with excellent catalytic performance. © 2015 Society of Chemical Industry
Innate immune response and implant loosening: Interferon gamma is inversely associated with early migration of total knee prostheses
Authors: Schoeman Monique A. E. , Pijls Bart G. , Oostlander Angela E. , Keurentjes Johan C. , Valstar Edward R. , Nelissen Rob G. H. H. , Meulenbelt Ingrid 
Published: July 2015

ABSTRACTTo allow prediction of the risk of loosening prior to surgery, we investigated the relationship between innate immune cytokine response via TLR2 stimulation and early migration of six different knee prostheses using RSA (radiostereometry). This study included 114 patients of a prospective RSA‐cohort who received a total knee arthroplasty. Whole blood cytokine responses were obtained by ex vivo stimulation with tripalmitoyl‐S‐glycerylcysteine (Pam3Cys‐SK4) for assessment of the TLR2 immune response. Early migration was calculated using the maximum total point motion (MTPM) 1 year post surgery. Principal component analysis (PCA) was applied to the cytokine data to reduce the correlated data of individual cytokines and identified two components. Subsequently, linear mixed model analyses were applied with adjustments for gender, age, BMI, time‐to‐blood sampling, and prosthesis type. Component 1, consisting of IFNγ, IL‐12p40, IL‐10, IL‐1β, TNFα, and IL‐6, showed a significant inverse association (β = −0.128; p = 0.041) with MTPM. Further analysis showed that IFNγ (β = −0.161, p = 0.008) had the highest contribution to this association and is particularly found in patients receiving another prosthesis than Nexgen (β = −0.239; p < 0.001). In conclusion, patients with high levels of IFNγ upon stimulation of TLR2 are at lower risk of early migration of their knee prosthesis. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:121–126, 2016.
Pathological role of fibroblast‐like synoviocytes in charcot neuroarthropathy
Authors: Molligan Jeremy , Barr Cameron , Mitchell Reed , Schon Lew , Zhang Zijun 
Published: July 2015

ABSTRACTThis study was designed to characterize the synovium in the joints of Charcot neuroarthropathy (CNA) and investigate the potential role of fibroblast‐like synoviocytes (FLS) in the pathology of CNA. Synovial samples were collected from CNA patients (n = 7) and non‐CNA patients (n = 7), for control, during orthopaedic procedures and used for histology and isolation of FLS. Histological characterization of synovium included innervation and FLS localization. The isolated FLS from the CNA and non‐CNA synovium were cultured, with or without tumor necrosis factor‐α (TNF‐α), for evaluation of invasiveness, gene expression, and cartilage degradation. Vasoactive intestinal peptide (VIP), a neuropeptide, was supplemented into the co‐cultures of FLS and cartilage explants. Compared with the non‐CNA synovium, CNA synovium was highly inflammatory, with reduced innervation and intense expression of cadherin‐11. The FLS isolated from CNA synovium, particularly when activated with TNF‐α, were more invasive, increased the expression of ADAMTS4, IL‐6, and RANKL, and depleted proteoglycans from cartilage explants when they were co‐cultured. Addition of VIP into the culture medium neutralized the catabolic effect of the CNA FLS on cartilage explants. In conclusion, FLS plays an important role in the pathology of CNA. Therapies targeting synovium and FLS may prevent or treat the joint destruction in CNA. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:224–230, 2016.
Evaluation of stair motion contributes to new insights into hip osteoarthritis‐related motion pathomechanics
Authors: Meyer Christophe A.G. , Corten Kristoff , Fieuws Steffen , Deschamps Kevin , Monari Davide , Wesseling Mariska , Simon Jean‐Pierre , Desloovere Kaat 
Published: July 2015

ABSTRACTStair motion in the presence of hip osteoarthritis (OA) has received less attention than level walking. Its more strenuous aspect may shed the light on different locomotor strategies when compared to walking. We, therefore, aimed to define stair motion features associated to hip OA and to evaluate whether these specific features would differ from level walking and better characterize the hip pathological condition. Principal component and linear discriminant analyses were, respectively, used as data reduction and classification techniques. Our study highlighted that most of stair motion features associated to hip OA were similar to the ones of walking. Stair descent presented with the lowest misclassification error rate, ranging from 12% to 19% (estimated by cross‐validation). But, features that may be considered as a mechanism to reduce demand on the hip abductors were found to be more important in the stair ascent condition. This was reflected by both, greater importance in the classification rule and variance compared with walking, that is, decreased hip internal rotation moment at mid‐stance (72.50% vs. 57.63%) and increased trunk lateroflexion toward affected side (56.43% vs. 29.37%). This study emphasized the importance of investigating stair motion in hip osteoarthritic population by highlighting specific locomotor strategies. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:187–196, 2016.
Static and dynamic compression application and removal on the intervertebral discs of growing rats
Authors: Ménard Anne‐Laure , Grimard Guy , Massol Elise , Londono Irène , Moldovan Florina , Villemure Isabelle 
Published: July 2015

ABSTRACTFusionless implants are used to correct pediatric progressive spinal deformities, most of them spanning the intervertebral disc. This study aimed at investigating the effects of in vivo static versus dynamic compression application and removal on discs of growing rats. A microloading device applied compression. 48 immature rats (28 d.o.) were divided into two groups (43d, 53d). Each group included four subgroups: control (no surgery), sham (device installed without loading), static (0.2 MPa) and dynamic compressions (0.2 MPa ± 30% with 0.1 Hz). In 43d subgroups, compression was applied for 15 days. In 53d subgroups, compression was followed by 10 days without loading. Disc heights, nucleus/annulus volumetric proportions and nucleus proteoglycan contents were analyzed using one‐way ANOVA and post‐hoc Tukey comparisons (p < 0.05). Disc heights of 43d and 53d static and dynamic loading rats were lower than shams (p < 0.05). Volumetric proportions remained similar. At 43d, nucleus proteoglycan contents increased in both static and dynamic loading rats. However, at 53d, static loading rats had lower proteoglycan content than dynamic loading rats (p < 0.05). Disc structure is altered following static compression removal, but nucleus proteoglycan content remaining elevated in dynamic group. Dynamic fusionless implants would better preserve disc integrity. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:290–298, 2016.
Novel intracellular N‐terminal truncated matrix metalloproteinase‐2 isoform in skeletal muscle ischemia‐reperfusion injury
Authors: Joshi Sunil K. , Lee Lawrence , Lovett David H. , Kang Heejae , Kim Hubert T. , Delgado Cynthia , Liu Xuhui 
Published: July 2015

ABSTRACTIschemia‐reperfusion injury (IRI) occurs when blood returns to tissues following a period of ischemia. Reintroduction of blood flow results in the production of free radicals and reactive oxygen species that damage cells. Skeletal muscle IRI is commonly seen in orthopedic trauma patients. Experimental studies in other organ systems have elucidated the importance of extracellular and intracellular matrix metalloproteinase‐2 (MMP‐2) isoforms in regulating tissue damage in the setting of oxidant stress resulting from IRI. Although the extracellular full‐length isoform of MMP‐2 (FL‐MMP‐2) has been previously studied in the setting of skeletal muscle IRI, studies investigating the role of the N‐terminal truncated isoform (NTT‐MMP‐2) in this setting are lacking. In this study, we first demonstrated significant increases in FL‐ and NTT‐MMP‐2 gene expression in C2C12 myoblast cells responding to re‐oxygenation following hypoxia in vitro. We then evaluated the expression of FL‐ and NTT‐MMP‐2 in modulating skeletal muscle IRI using a previously validated murine model. NTT‐MMP‐2, but not FL‐MMP‐2 expression was significantly increased in skeletal muscle following IRI. Moreover, the expression of toll‐like receptors (TLRs) ‐2 and ‐4, IL‐6, OAS‐1A, and CXCL1 was also significantly up‐regulated following IRI. Treatment with the potent anti‐oxidant pyrrolidine dithiocarbamate (PDTC) significantly suppressed NTT‐MMP‐2, but not FL‐MMP‐2 expression and improved muscle viability following IRI. This data suggests that NTT‐MMP‐2, but not FL‐MMP‐2, is the major isoform of MMP‐2 involved in skeletal muscle IRI. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 34:502–509, 2016.
Assessment of polyaromatic hydrocarbons and pesticide residues in domestic and imported pangasius (Pangasianodon hypophthalmus) fish in India
Authors: Chatterjee Niladri Sekhar , Banerjee Kaushik , Utture Sagar , Kamble Narayan , Rao B Madhusudana , Panda Satyen Kumar , Mathew Suseela 
Published: July 2015

AbstractBACKGROUNDThe pangasius catfish (Pangasianodon hypophthalmus) is a ubiquitous item of seafood in global markets. However, pesticide residues in aquaculture fish, arising from agricultural run‐off and/or direct application during pond preparation, pest control and harvest, are a potential food safety concern. This study assessed the level of chemical hazard in domestic and imported pangasius fish in India.RESULTSA total of 119 contaminants, including polyaromatic hydrocarbons (PAHs), organochlorines (OCs) and other groups of pesticides, were screened in 148 samples during 2014–2015 as the first endeavour of its kind in an Indian context, employing a validated gas chromatography/tandem mass spectrometry (GC/MS/MS) method. Apart from the low‐level incidence of OCs, pesticides such as quinalphos, malathion, parathion‐methyl, etc. were detected in 38% of the samples. In comparison with Indian pangasius, fewer contaminants at low residue level were detected in pangasius fillets imported from Vietnam.CONCLUSIONThe human dietary exposures of the residue concentrations detected were less than the maximum permissible intakes and hence appeared safe. However, detection of commonly used pesticides indicated their direct application in aquaculture and contamination from agricultural run‐off. This emphasizes the need for continual residue monitoring in aquaculture fish. © 2015 Society of Chemical Industry
Proximate composition and nutritional quality of deep sea growth sea cucumbers (Stichopus japonicus) from different origins
Authors: Gao Yue , Li Zhibo , Qi Yanxia , Guo Zhenyu , Lin Yantong , Li Wei , Hu Yucai , Zhao Qiancheng 
Published: July 2015

AbstractBACKGROUNDDeep sea growth sea cucumber (Stichopus japonicus) (DSG‐SC) is considered a most nutritious and luxurious seafood in Asia. This study compared the proximate composition and nutritional quality of collagen, polysaccharides, amino acids (AAs) and fatty acids (FAs) in DSG‐SCs from different origins.RESULTSThe contents of protein, ash, carbohydrate, fat, collagen, saturated fatty acids (SFAs), monounsaturated fatty acids (MUFAs), polyunsaturated fatty acids (PUFAs), total amino acids (TAAs), essential amino acids (EAAs), fucose and uronic acid differed among the origins. DSG‐SC of Dalian origin had lower contents of ash, fat, uronic acid, TAAs and EAAs but higher contents of protein, collagen, PUFAs, eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and fucose compared with the other origins. DSG‐SCs had a higher proportion of PUFAs and were richer in polysaccharides than other seafood. Glutamate and glycine were the dominant AAs, while leucine and threonine were the most abundant EAAs.CONCLUSIONDSG‐SCs are a good source of collagen, polysaccharides (especially fucose), EAAs (especially leucine and threonine) and PUFAs (especially EPA and DHA). Dalian seems to be a promising origin to produce high‐value sea cucumber with high PUFA, fucose, collagen and protein contents. © 2015 Society of Chemical Industry
Preharvest temperature affects chilling injury in dessert bananas during storage
Authors: Bugaud Christophe , Joannès‐Dumec Charlène , Louisor Jacques , Tixier Philippe , Salmon Frédéric 
Published: July 2015

AbstractBACKGROUNDThe effect of temperature on chilling injury during fruit growth was studied in a new banana hybrid CIRAD925 in which seasonal variability in chilling susceptibility was observed when fruits were stored at 13 °C.RESULTSThe relationship between the response to chilling (presence/absence) and the temperature during banana fruit growth was examined with a logistic regression model. An explanatory variable XN,P was defined as the mean temperature during a period, expressed in weeks, which began N week(s) after flowering and lasted P week(s). The model was calibrated with 143 bunches with a green life of 30 ± 5 days and validated with 156 bunches grown in six plots under different growing conditions. Chilling injury was best predicted by the mean temperature during the period beginning 1 week after flowering and lasting 5 weeks (X1,5). Above a mean temperature of 24.1 °C in the period concerned, banana fruits had a 95% probability of chilling injury at 13 °C. Below a temperature of 23.4 °C, banana fruits only had a 5% probability of chilling injury.CONCLUSIONThe results provide a tool to predict chilling susceptibility in banana fruit whatever the thermal conditions in tropical regions. © 2015 Society of Chemical Industry