bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Versatile Approach for the Site‐Specific Modification of Recombinant Antibodies Using a Combination of Enzyme‐Mediated Bioconjugation and Click Chemistry
Authors: Alt Karen , Paterson Brett M. , Westein Erik , Rudd Stacey E. , Poniger Stan S. , Jagdale Shweta , Ardipradja Katie , Connell Timothy U. , Krippner Guy Y. , Nair Ashish K. N. , Wang Xiaowei , Tochon‐Danguy Henri J. , Donnelly Paul S. , Peter Karlheinz , Hagemeyer Christoph E. 
Published: May 2015

AbstractA unique two‐step modular system for site‐specific antibody modification and conjugation is reported. The first step of this approach uses enzymatic bioconjugation with the transpeptidase Sortase A for incorporation of strained cyclooctyne functional groups. The second step of this modular approach involves the azide–alkyne cycloaddition click reaction. The versatility of the two‐step approach has been exemplified by the selective incorporation of fluorescent dyes and a positron‐emitting copper‐64 radiotracer for fluorescence and positron‐emission tomography imaging of activated platelets, platelet aggregates, and thrombi, respectively. This flexible and versatile approach could be readily adapted to incorporate a large array of tailor‐made functional groups using reliable click chemistry whilst preserving the activity of the antibody or other sensitive biological macromolecules.
Nano‐sio2‐reinforced ultraviolet‐curing materials for three‐dimensional printing
Authors: Zhang Congchao , Cui Yihua , Li Juan , Jiang Dandan 
Published: May 2015

ABSTRACTAs an additive manufacturing technology, ultraviolet (UV)‐curing three‐dimensional printing, which requires the use of a photocurable resin, is increasingly being used to produce customized end‐user parts of many complex shapes. In this study, to improve the strength and ductility of printing materials, nano‐SiO2‐reinforced photocurable resins were prepared by a planetary ball mill; then, the morphology, photochemistry, thermal property, and mechanical properties of the nanocomposites were investigated and characterized. Transmission electron microscopy analysis indicated that the modified nano‐SiO2 was well dispersed in the photocurable resin. The glass‐transition temperature increased from 67.2°C for the unfilled resin to 71.7 and 80.1°C for nanocomposites with nano‐SiO2 contents of 0.3 and 0.7 wt %, respectively. The tensile strength and impact strength were increased by 46.7 and 165.3% for nanocomposites with 0.3 wt % nano‐SiO2. The flexural modulus of the nanocomposites increased from 1.7 to 8.0 GPa when 0.7 wt % nano‐SiO2 was added to the photocurable resin; this appeared to originate from the relatively high level of dispersion and the intimate combination of the nano‐SiO2 with the matrix. The investigation of the physical and chemical properties of such UV‐curing materials showed that the low filler concentration (<1 wt %) of nano‐SiO2 did not affect the processability of the nanocomposites. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42307.
Biodegradable antimicrobial films based on poly(lactic acid) matrices and active azo compounds
Authors: Concilio Simona , Iannelli Pio , Sessa Lucia , Olivieri Rita , Porta Amalia , De Santis Felice , Pantani Roberto , Piotto Stefano 
Published: May 2015

ABSTRACTUsing solvent casting and melt compounding methods, we realized antibacterial and antifungal poly(lactic acid)‐based films by introducing different percentages of antimicrobial azo dyes into polymer matrices. Concentration up to 0.01% (w/w) of azo compounds permitted the preparation of antimicrobial and transparent films. The thin films retained the properties of the pure PLA matrices, such as glass transition temperature, flexibility, and amorphous nature. The films exhibited antimicrobial activity and the capability to inhibit biofilms formation of Staphylococcus aureus and Candida albicans. Spectrophotometric investigation of azo compounds release from the polymer matrices confirmed that the materials might have applications in fields where an intrinsic antimicrobial ability of the material is required, such as biomedical tools, biodegradable antibacterial coatings, and films for active packaging. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42357.
Attachment ability of two invasive amphipod species may promote their spread by overland transport
Authors: Bacela‐Spychalska K. 
Published: May 2015

Abstract

Amphipods are very successful invaders in European inland waters including lakes.
There are several vectors identified as responsible for amphipod species introductions and spread, with overland transport increasingly recognized as particularly important, especially in conjunction with transport between sites of sailing and/or angling activity.
In this study two species of invasive gammarids with a wide distribution range in Europe, Dikerogammarus haemobaphes and Pontogammarus robustoides, were tested for their ability to attach and remain adhered to sailing rope.
The results of the experiment show that both species may have high potential to be transported with sailing/angling equipment, as the percentage of individuals remaining attached to the rope after 30 s of ‘holding’ followed by 30 s of ‘shaking’ was very high (24% and 17%, for D. haemobaphes and P. robustoides, respectively).
The necessity for preventative measures to stop translocation of these species between European water bodies is emphasized and discussed.
Copyright © 2015 John Wiley & Sons, Ltd.
Verapamil hepatic clearance in four preclinical rat models: towards activity‐based scaling
Authors: Nicolaï J. , De Bruyn T. , Van Veldhoven P. P. , Keemink J. , Augustijns P. , Annaert P. 
Published: May 2015

AbstractThe current study was designed to cross‐validate rat liver microsomes (RLM), suspended rat hepatocytes (SRH) and the isolated perfused rat liver (IPRL) model against in vivo pharmacokinetic data, using verapamil as a model drug. Michaelis‐Menten constants (Km), for the metabolic disappearance kinetics of verapamil in RLM and SRH (freshly isolated and cryopreserved), were determined and corrected for non‐specific binding. The ‘unbound’ Km determined with RLM (2.8 µ m) was divided by the ‘unbound’ Km determined with fresh and cryopreserved SRH (3.9 µ m and 2.1 µ m, respectively) to calculate the ratio of intracellular to extracellular unbound concentration (Kpu,u). Kpu,u was significantly different between freshly isolated (0.71) and cryopreserved (1.31) SRH, but intracellular capacity for verapamil metabolism was maintained after cryopreservation (200 vs. 191 µl/min/million cells). Direct comparison of intrinsic clearance values (Clint) in RLM versus SRH, yielded an activity‐based scaling factor (SF) of 0.28–0.30 mg microsomal protein/million cells (MPPMC). Merging the IPRL‐derived Clint with the MPPMC and SRH data, resulted in scaling factors for MPPGL (80 and 43 mg microsomal protein/g liver) and HPGL (269 and 153 million cells/g liver), respectively. Likewise, the hepatic blood flow (61 ml/min/kg b.wt) was calculated using IPRL Clint and the in vivo Cl. The scaling factors determined here are consistent with previously reported CYP450‐content based scaling factors. Overall, the results show that integrated interpretation of data obtained with multiple preclinical tools (i.e. RLM, SRH, IPRL) can contribute to more reliable estimates for scaling factors and ultimately to improved in vivo clearance predictions based on in vitro experimentation. Copyright © 2015 John Wiley & Sons, Ltd.
Micelle‐Enhanced Bioorthogonal Labeling of Genetically Encoded Azido Groups on the Lipid‐Embedded Surface of a GPCR
Authors: Tian He , Sakmar Thomas P. , Huber Thomas 
Published: May 2015

AbstractGenetically encoded p‐azido‐phenylalanine (azF) residues in G protein‐coupled receptors (GPCRs) can be targeted with dibenzocyclooctyne‐modified (DIBO‐modified) fluorescent probes by means of strain‐promoted [3+2] azide–alkyne cycloaddition (SpAAC). Here we show that azF residues situated on the transmembrane surfaces of detergent‐solubilized receptors exhibit up to 1000‐fold rate enhancement relative to azF residues on water‐exposed surfaces. We show that the amphipathic moment of the labeling reagent, consisting of hydrophobic DIBO coupled to hydrophilic Alexa dye, results in strong partitioning of the DIBO group into the hydrocarbon core of the detergent micelle and consequently high local reactant concentrations. The observed rate constant for the micelleenhanced SpAAC is comparable with those of the fastest bioorthogonal labeling reactions known. Targeting hydrophobic regions of membrane proteins by use of micelle‐enhanced SpAAC should expand the utility of bioorthogonal labeling strategies.
Outcomes of transcatheter aortic valve replacement in patients with chronic liver disease
Authors: Shah Anoop M. , Ogbara Jeffrey , Herrmann Howard C. , Fox Zachary , Kadakia Mitul , Anwaruddin Saif , Bavaria Joseph E. , Desai Nimesh D. , Jagasia Dinesh , Szeto Wilson Y. , Li Robert H. , Vallabhajosyula Prashanth , Giri Jay 
Published: May 2015

ObjectiveTranscatheter aortic valve replacement (TAVR) has emerged as an alternative to high‐risk surgery in patients with comorbid conditions. The role of TAVR in patients with liver disease has not been examined.MethodsWe examined the procedural and intermediate to long‐term follow‐up outcomes of patients with severe symptomatic aortic valve stenosis and chronic liver disease, identified by liver biopsy or from a combination of clinical findings. All patients were treated with balloon‐expandable transfemoral (TF) or transapical (TA) TAVR between November 2007 and February 2014.ResultsA total of 17 of 706 (2.41%) patients treated at our institution with TF [n = 14] or TA [n = 3] TAVR had chronic liver disease (mean age 77.65 ± 9.06 years, 7 women, mean STS score 8.37, mean Charlson score 5.00, mean MELD score 11.35, Child–Turcotte–Pugh (CTP) Class A [n = 11], B [n = 6], C [n = 0], biopsy proven liver disease [n = 5]). Median follow‐up was 466 days (range = 12–1,403 days). The mean post‐procedure length of hospital stay was 5.88 ± 3.08 days. Procedural success was achieved in all cases. In‐hospital mortality was 5.88% and 90‐day mortality was 17.65%. Safety and efficacy endpoints as defined by the valve academic research consortium (VARC) were significant for one perioperative death from a proximate cardiac cause (post‐operative day 14), one death after hospital discharge of unknown cause (post‐operative day 12), two late deaths from non‐cardiac causes (post‐operative days 50 and 487, respectively), and one late death of unknown cause (post‐operative day 1,005). There were no life‐threatening or major bleeding complications. One patient had an MI, one had a transient ischemic attack, four had transient, Stage I, acute kidney injury and one had transient, Stage II, acute kidney injury.ConclusionTF and TA TAVR are feasible methods for treating aortic stenosis in patients with chronic liver disease. In patients with mild to moderate chronic liver disease there are acceptable rates of early and late complications, however, outcomes in patients with advanced liver disease (MELD >20 or CTP class C) warrant further study. © 2015 Wiley Periodicals, Inc.
Vascular brachytherapy versus drug‐eluting stents in the treatment of in‐stent restenosis: A meta‐analysis of long‐term outcomes
Authors: Benjo Alexandre , Cardoso Rhanderson N. , Collins Tyrone , Garcia Daniel , Macedo Francisco Y. , El‐Hayek Georges , Nadkarni Girish , Aziz Emad , Jenkins J. Stephen 
Published: May 2015

IntroductionClinical trials have shown a short‐term benefit of drug‐eluting stents (DES) compared to vascular brachytherapy (VBT) for treatment of in‐stent restenosis (ISR). The long‐term benefits of DES vs. VBT are conflicting in the literature. This study aimed to do a meta‐analysis of long‐term outcomes of DES compared to VBT for treatment of ISR.MethodsPubMed, EMBASE, Cochrane Central and unpublished data were searched for cohort studies and randomized controlled trials (RCTs) that directly compared VBT to DES for the treatment of ISR. We evaluated the following outcomes at 2–5 years of follow‐up: target lesion revascularization (TLR), target vessel revascularization (TVR), myocardial infarction (MI), stent thrombosis, cardiovascular (CV) mortality, and overall mortality. Heterogeneity was defined as I2 values > 25%. Review Manager 5.1 was used for statistical analysis.ResultsWe included 1,375 patients from five studies, of which three were RCTs. VBT was used to treat ISR in 685 (49.8%) patients. After a 2–5 year follow‐up, no significant differences were found between treatment groups regarding MI (P = 0.49), stent thrombosis (P = 0.86), CV mortality (P = 0.35), and overall mortality (P = 0.71). TLR (OR 2.37; CI 1.55–3.63; P < 0.001) and TVR (OR 2.23; CI 1.01–4.94; P = 0.05) were significantly increased in patients who received VBT.ConclusionThis study suggests that DES are associated with decreased long‐term revascularization procedures when compared to VBT for the treatment of ISR. This benefit does not appear to be associated with a significant reduction in mortality or myocardial infarction. © 2015 Wiley Periodicals, Inc.
Midterm clinical outcome following Edwards SAPIEN or Medtronic Corevalve transcatheter aortic valve implantation (TAVI): Results of the Belgian TAVI registry
Authors: Collas Valérie M. , Dubois Christophe , Legrand Victor , Kefer Joëlle , De Bruyne Bernard , Dens Jo , Rodrigus Inez E. , Herijgers Paul , Bosmans Johan M. ,   for the Belgian TAVI Registry Participants
Published: May 2015

ObjectiveTo assess midterm (3 years) clinical outcomes of transcatheter aortic valve implantation (TAVI) in Belgium using the Edwards SAPIEN valve or the Medtronic CoreValve transcatheter heart valve (THV).BackgroundMedium and long term follow‐up data of both THVs are still relatively scarce, although of great clinical relevance for a relatively new but rapidly expanding treatment modality. Therefore, reporting mid‐ and long term clinical outcome data, coming from large “real world” national registries, remains contributive.MethodsBetween December 2007 and March 2012, 861 “real world” patients who were not candidates for surgical aortic valve replacement as decided by the local heart teams, underwent TAVI at 23 sites. Eleven sites exclusively used SAPIEN THV (n = 460), while 12 exclusively used CoreValve THV (n = 401). Differences in clinical outcomes by valve system were assessed, according to access route and baseline EuroSCORE risk profile (<10%: low, 10–20%: intermediate and >20%: high risk).ResultsOverall cumulative survival at 3 years was 51% for SAPIEN vs. 60% for CoreValve (P = 0.021). In transfemorally treated patients, SAPIEN and CoreValve had similar survival at 3 years for each of the baseline EuroSCORE cohorts (low risk: 72% vs. 76%, P = 0.45; intermediate risk: 62% vs. 59%, P = 0.94; high risk: 48% vs. 53%, P = 0.65).ConclusionCumulative midterm 3 year survival after transfemoral TAVI in “real world” patients refused for surgery with similar baseline EuroSCORE risk profile is not different between SAPIEN or CoreValve. © 2015 Wiley Periodicals, Inc.
Hybrid Conducting Biofilm with Built‐in Bacteria for High‐Performance Microbial Fuel Cells
Authors: Zhao Cui‐e , Wu Jiansheng , Ding Yuanzhao , Wang Victor Bochuan , Zhang Yingdan , Kjelleberg Staffan , Loo Joachim Say Chye , Cao Bin , Zhang Qichun 
Published: May 2015

AbstractThe front cover artwork is provided by the group of Qichun Zhang at Nanyang Technology University (Singapore). The image shows the extracellular electron transfer between a graphene–carbon nanotube hybrid biofilm anode and­ Shewanella oneidensis­ cells. Read the full text of the Communication at10.1002/celc.201402458.
Microfoundations of institutional change: Contrasting institutional sabotage to entrepreneurship
Authors: Lakshman C. , Akhter Manzoom 
Published: May 2015

AbstractThis paper addresses little understood microfoundations of institutionally driven organizational change and utilizes an institutional‐conflict‐based approach to examine innovation in organizational forms. Using a two‐case comparative analysis, we longitudinally examine the antecedents, mechanisms, and success/failure of attempts at change by institutional entrepreneurs. We analyze and develop theoretical insights on the interplay between internal political processes and external competitive actions in the creation of innovation in organizational forms and the subsequent legitimacy struggles through which an organizational field evolves in a sports (cricket) business context. We draw implications for institutional actors by observing patterns in organizational and institutional evolution in such contexts. We contribute to institutional entrepreneurship literature by developing a nuanced process model of success and failure in institutional entrepreneurship. Copyright © 2015 ASAC. Published by John Wiley & Sons, Ltd.
Enhanced External Counterpulsation Is Cost‐Effective in Reducing Hospital Costs in Refractory Angina Patients
Authors: Lawson William E. , Hui John C.K. , Kennard Elizabeth D. , Linnemeier Georgiann ,   for the IEPR‐II Investigators
Published: May 2015

ABSTRACTBackgroundEnhanced external counterpulsation (EECP) is effective in the treatment of refractory angina, a condition suffered by 1.7 million Americans. Declining cardiovascular mortality and appropriate use criteria may further increase this number.HypothesisEECP is hypothesized to be cost‐effective in reducing hospitalizations in refractory angina patients.MethodsThe data used in this analysis were collected in phase II of the International EECP Patient Registry (IEPR‐II). Data were collected on changes in Canadian Cardiovascular Society functional class, Duke Activity Status Index, and number of hospitalizations in the 6 months prior to EECP and in the 6‐ and 12‐month intervals following EECP. Estimates of the changes in annual cost of all‐cause hospitalization before and after EECP therapy were calculated by the product of the differences in hospitalization rates in the 6‐month interval before and after EECP treatment and estimated hospitalization and physician charges after subtracting the average cost of EECP.ResultsData for 1015 patients were analyzed. Hospitalization occurred in 55.2% of patients, an average of 1.7 ± 1.4 hospitalizations/patient, in the 6‐month period before 35 hours of EECP; and in 24.4%, an average of 1.4 ± 1.0 hospitalizations/patient, during the 6‐ to 12‐month period after EECP. The average hospitalization and physician charge in the US was $17 995, and the average EECP cost was $4880, yielding an annual cost savings/patient of $17 074.ConclusionsTreatment of refractory angina patients with EECP resulted in improvement in angina and functional class accompanied by a sustained reduction in health care costs over 1 year of follow‐up.
Quality of Anticoagulation With Vitamin K Antagonists
Authors: Bertomeu‐González Vicente , Anguita Manuel , Moreno‐Arribas José , Cequier Ángel , Muñiz Javier , Castillo‐Castillo Jesús , Sanchis Juan , Roldán Inmaculada , Marin Francisco , Bertomeu‐Martínez Vicente ,   on behalf of the FANTASIIA Study Investigators
Published: May 2015

ABSTRACTBackgroundVitamin K antagonists (VKA) have a narrow therapeutic range, and literature analysis reveals poor quality of anticoagulation control. We sought to assess the prevalence of poor anticoagulant control in patients under VKA treatment in the prevention of stroke for atrial fibrillation (AF).HypothesisControl of anticoagulation with VKA is inadequate in a high percentage of patients with AF.MethodsPatients with AF under VKA treatment were prospectively recruited in this observational registry. The sample comprised 948 patients. The estimated time spent in the therapeutic range (TTR) was calculated, and variables related with a TTR >65% were analyzed.ResultsMean age was 73.8 ± 9.4 years, and 42.5% of the patients were women. Mean TTR was 63.77% ± 23.80% for the direct method and 60.27% ± 24.48% for the Rosendaal method. Prevalence of poor anticoagulation control was 54%. Variables associated with good anticoagulation control were university studies (odds ratio [OR]: 1.99, 95% confidence interval [CI]: 1.08‐3.64), chronic hepatic disease (OR: 8.15, 95% CI: 1.57‐42.24), low comorbidity expressed as Charlson index (OR: 0.87, 95% CI: 0.76‐0.99), no previous cardiac disease (OR: 0.64, 95% CI: 0.41‐0.98), lower risk of bleeding assessed as hypertension, abnormal renal/liver function, stroke, bleeding history or predisposition, labile international normalized ratio, elderly age, and use of drugs or alcohol (HAS‐BLED; OR: 0.81, 95% CI: 0.69‐0.95), and lower heart rate (OR: 0.99, 95% CI: 0.98‐0.99).ConclusionsPatients who receive VKA to prevent stroke for AF spend less than half the time within therapeutic range.
Environmental Transmission Electron Microscopy Study of Diesel Carbon Soot Combustion under Simulated Catalytic‐Reaction Conditions
Authors: Mori Kohsuke , Watanabe Keitaro , Sato Takeshi , Yamashita Hiromi 
Published: May 2015

AbstractThe front cover artwork is provided by the groups of Prof. Kohsuke Mori and Prof. Hiromi Yamashita (Osaka University) in collaboration with Keitaro Watanabe and Dr. Takeshi Sato (Hitachi High‐Technologies). The cover picture shows the time‐lapsed TEM images acquired by environmental transmission electron microscopy (ETEM) observation during the exposure of the catalysts and soot to O2 at elevated temperatures. Read the full text of the article at 10.1002/cphc.201402818.
Memory and executive functions in persons with type 2 diabetes: a meta‐analysis
Authors: Sadanand Shilpa , Balachandar Rakesh , Bharath Srikala 
Published: May 2015

SummaryLiterature suggests that persons with type 2 diabetes mellitus (T2DM) are at risk for cognitive impairment, hence dementia. Common domains reported to be affected in those with T2DM are memory and executive functions. The extent of influence of T2DM on these domains has varied among studies. A systematic review and meta‐analysis was carried out to understand whether sub‐domains contributed to the variations observed in published research. We searched ‘PubMed’, ‘ScienceDirect’, ‘SciVerseHub’, ‘Psychinfo’, ‘Proquest’ ‘Ebsco’ and ‘J‐gate Plus’ databases for published studies on cognition and T2DM among persons aged 50 years and older. Memory, executive functions and processing speed domain and sub‐domain scores were extracted; effect sizes (Cohen's d) were calculated and analysed. Eight hundred seventeen articles were found. After various levels of filtering, 15 articles met the inclusion criteria for quantitative analyses. The analyses indicated that in comparison to controls, persons with T2DM showed decrements in episodic memory (d = −0.51), logical memory (d = −0.24), sub‐domain of executive functions which included phonemic fluency (d = −0.35) and cognitive flexibility (d = 0.52), and speed of processing (d = −0.22). We found no difference in the sub‐domains of verbal short‐term memory and working memory. The meta‐analysis revealed a detrimental effect of T2DM on cognitive sub‐domains, namely, episodic memory and cognitive flexibility. There was a trend for the logical memory, phonemic fluency and processing speed to be affected. The analysis indicates that T2DM is a detrimental factor on certain cognitive sub‐domains, rendering the person vulnerable to subsequent dementia. Copyright © 2016 John Wiley & Sons, Ltd.