bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of tidal range and coastline morphology on the evolution of two macrotidal sand spits
Authors: Tessier Bernadette , Poirier Clément , Fruergaard Mikkel , Chaumillon Eric , Weill Pierre , Bertin Xavier , Mouazé Dominique 
Published: July 2024

AbstractThe present study examines the stratigraphy of two sandspits that both appeared and developed since the middle of the 17th century during the Little Ice Age. The Arçay spit is located along the macrotidal Atlantic coast in South‐West France with a maximum tidal range of 6.5 m. The Pointe du Banc in the English Channel, North‐West France, is located in a hypertidal coastal setting with a tidal range of up to 14 m. The evolution of the two spit systems has been compared using historical maps, ground penetrating radar data and facies analysis and geochronological data from sediment cores. The Pointe du Banc spit developed between 1650 and 1750 CE, in a dominant seaward direction while the main mode of construction of the Arçay spit was in a longshore direction. It is proposed that this difference relates to sediment supply and coastline morphology. At the Pointe du Banc spit, the large tidal range causes a long wind fetch and sustained aeolian sediment supply. Moreover, the spit is located at the apex of a large‐scale embayment where sediment transport from north and south converges. Combined, these factors result in a positive sediment budget and seaward shoreline progradation. At the Arçay spit, high wave obliquity results in a large littoral drift and sustained longshore spit construction. At both locations, low gradient shorefaces may have favoured a net landward‐directed sediment flux that supply sand to the foreshore.
Development and validation of general plasma screening method for performance enhancing drugs in racehorses utilizing liquid chromatography–high‐resolution mass spectrometry (LC–HRMS)
Authors: Lomnicka Izabela , Dubey Saurabh , Waller Pamela , Vora Dharmikkumar , Dirikolu Levent 
Published: July 2024

AbstractThe screening of drugs in plasma and urine often requires initial extraction (such as liquid–liquid extraction and solid‐phase extraction) before the samples are submitted to instrumental analyses. These extraction procedures are often laborious and time‐consuming. In this manuscript, a high‐throughput automated assay based on liquid chromatography–high‐resolution mass spectrometry (LC–HRMS) suitable for use as an initial testing procedure covering multiple classes of compounds prohibited in horse racing is described. The assay requires a 600‐μL plasma aliquot, which is subjected to solid phase extraction (SPE) using OASIS HLB 96‐well SPE with Biotage Extrahera system, evaporation, and reconstitution in a 96‐well collection plate. LC–HRMS analyses were carried out on a Thermo Q‐Exactive Mass spectrometer coupled with Thermo UHPLC system equipped with Thermo Accela ALS 2.4.0 autosampler linked to ACE Excel column. Drug targets were detected by retention time and accurate mass, with a mass tolerance window of 5 ppm in positive and negative ionization mode. The screening method was validated for over 300 drug targets in a 13‐min run. Validation data including sensitivity, specificity, extraction recovery, and precision are presented. As the method employs full‐scan mass spectrometry, unlimited number of drug targets can theoretically be incorporated into this method.
Rates and predictors of cardiovascular and non‐cardiovascular outcomes in heart failure with preserved ejection fraction
Authors: Shahim Angiza , Donal Erwan , Hage Camilla , Oger Emmanuel , Savarese Gianluigi , Persson Hans , Haugen‐Löfman Ida , Ennezat Pierre‐Vladimir , Sportouch‐Dukhan Catherine , Drouet Elodie , Daubert Jean‐Claude , Linde Cecilia , Lund Lars H. ,   the KaRen investigators
Published: July 2024

AbstractAimsThe detailed sub‐categories of death and hospitalization, and the impact of comorbidities on cause‐specific outcomes, remain poorly understood in heart failure (HF) with preserved ejection fraction (HFpEF). We sought to evaluate rates and predictors of cardiovascular (CV) and non‐CV outcomes in HFpEF.MethodsThe Karolinska–Rennes study was a bi‐national prospective observational study designed to characterize HFpEF (ejection fraction ≥45%). Patients were followed for cause‐specific death and hospitalization. Baseline characteristics were pre‐selected based on clinical relevance and potential eligibility criteria for HFpEF trials. The associations between characteristics and cause‐specific outcomes were assessed with univariable and multivariable Cox regressions.ResultsFive hundred thirty‐nine patients [56% females; median (inter‐quartile range) age 79 (72–84) years; NT‐proBNP/BNP 2448 (1290–4790)/429 (229–805) ng/L] were included. Over 1196 patient‐years follow‐up [median (min, max) 744 days (13–1959)], there were 159 (29%) deaths (13 per 100 patient‐years: CV 5.1 per 100, dominated by HF 3.9 per 100; and non‐CV 5.8 per 100, dominated by cancer, 2.3 per 100). There were 723 hospitalizations in 338 patients (63%; 60 per 100 patient‐years: CV 33 per 100, dominated by HF 17 per 100; and non‐CV 27 per 100, dominated by lung disease 5 per 100).Higher age and natriuretic peptides, lower serum natraemia and NYHA class III–IV were independent predictors of CV death; lower serum natraemia, anaemia and stroke of non‐CV death; and anaemia and lower serum natraemia of non‐CV death or hospitalizations. There were no apparent predictors of CV death or hospitalization.ConclusionsIn a clinical cohort hospitalized and diagnosed with HFpEF, death and hospitalization rates were roughly similar for CV and non‐CV causes. CV deaths were predicted primarily by severity of HF; non‐CV deaths primarily by anaemia and prior stroke. Lower serum sodium predicted both. Hospitalizations were difficult to predict.
Biomarker profiles associated with reverse ventricular remodelling in patients with heart failure and a reduced ejection fraction: Insights from the echocardiographic substudy of the VICTORIA trial
Authors: Tromp Jasper , Lam Carolyn S.P. , Alemayehu Wendimagegn , de Filippi Christopher R. , Melenovský Vojtěch , Sliwa Karen , Lopatin Yuri , Arango Juan Luis , Bahit M. Cecilia , Roessig Lothar , O'Connor Christopher M. , Shah Palak , Westerhout Cynthia M. , Voors Adriaan A. , Pieske Burkert , Armstrong Paul W. ,   for the VICTORIA Study Group
Published: July 2024

AbstractAimsReverse ventricular remodelling, defined as a decrease in left ventricular end‐systolic volume indexed to body surface area (LVESVI) or an increase in left ventricular ejection fraction (LVEF), is associated with improved clinical outcomes in patients with heart failure with reduced ejection fraction (HFrEF). However, the underlying pathophysiological mechanisms remain unclear.Methods and resultsWe evaluated paired core‐lab assessed echocardiograms and measurements of 92 biomarkers at baseline and 8 months thereafter in 419 participants with HFrEF. Reverse ventricular remodelling was defined as a >5% LVEF increase or >15% LVESVI relative decrease between baseline and 8 months. We evaluated the association between baseline biomarkers and their changes with reverse ventricular remodelling in the prospectively randomized controlled VICTORIA (Vericiguat Global Study in Subjects With Heart Failure With Reduced Ejection Fraction) trial. Of 419 patients (median age 66 [interquartile range 57–74] years, 27.4% women), 206 (49.2%) had reverse ventricular remodelling (either a 5% LVEF increase or a 15% LVESVI decrease). There were no differences in baseline biomarker concentrations between patients with versus those without reverse ventricular remodelling on follow‐up. However, in patients with reverse ventricular remodelling there were significant decreases in biomarkers relating to inflammation and cardiac metabolism; particularly the tumour necrosis factor superfamily member 13B (ratio 0.82, 95% confidence interval [CI] 0.77–0.88), growth differentiation factor‐15 (ratio 0.74, 95% CI 0.66–0.84), and insulin‐like growth factor binding protein 7 (ratio 0.80, 95% CI 0.73–0.88).ConclusionsReverse ventricular remodelling in patients with HFrEF is associated with a decrease of biomarkers related to inflammation and cardiac metabolism.
Status and timing of angiotensin receptor–neprilysin inhibitor implementation in patients with heart failure and reduced ejection fraction: Data from the Swedish Heart Failure Registry
Authors: Stolfo Davide , Benson Lina , Lindberg Felix , Dahlström Ulf , Käck Oskar , Sinagra Gianfranco , Lund Lars H. , Savarese Gianluigi 
Published: July 2024

AimsWe explored timing, settings and predictors of angiotensin receptor–neprilysin inhibitor (ARNI) initiation in a large, nationwide cohort of patients with heart failure (HF) with reduced ejection fraction (HFrEF).Methods and resultsPatients with HFrEF (ejection fraction <40%) registered in the Swedish HF Registry in 2017–2021 and naïve to ARNI were evaluated for timing and location of, and their characteristics at ARNI initiation. ARNI use increased from 8.3% in 2017 to 26.7% in 2021. Among 3892 hospitalized patients, 8% initiated ARNI in‐hospital or ≤14 days after discharge, 4% between 15 and 90 days, and 88% >90 days after discharge or never initiated. Factors associated with earlier initiation included follow‐up in specialized HF care, more severe HF, previous HF treatment use and higher income, whereas older age, higher comorbidity burden and living alone were associated with later/no initiation. Of 16 486 HFrEF patients, 8.1% inpatients and 5.9% outpatients initiated an ARNI at the index date. Factors associated with initiation in outpatients were overall consistent with those linked with an in‐hospital/earlier ARNI initiation; 4.9% of 10 209 with HF duration <6 months and 9.1% of 5877 with HF duration ≥6 months initiated ARNI. Predictors of ARNI initiation in HF duration <6 months were inpatient status, lower ejection fraction, hypertension, whereas previous angiotensin‐converting enzyme inhibitor/angiotensin receptor blocker use was associated with less likely initiation. Discontinuation at 1 year ranged between 13% and 20% across the above‐reported analyses.ConclusionsIn‐hospital and early initiation of ARNI are limited in real‐world care but still slightly more likely than in outpatients. ARNI were more likely initiated in patients with more severe HF, which might suggest its use as a second‐line treatment and only following worsening of clinical status. One‐year discontinuation rates were consistent regardless of the timing/setting of ARNI initiation.
Fast and efficient extraction and determination of nonsteroidal anti‐inflammatory drugs using poly(8‐hydroxyquinoline)‐coated magnetic graphene oxide nanocomposite prior to capillary electrophoresis analysis in wastewater, breast milk, and urine samples
Authors: Shahsavani Abolfath , Fakhari Ali Reza 
Published: July 2024

AbstractIn this study, magnetic graphene oxide coated with poly(8‐hydroxyquinoline) was successfully synthesized, characterized, and utilized as a novel sorbent for the ultrasonic‐assisted dispersive magnetic solid‐phase extraction of naproxen and ibuprofen. These analytes served as representative analytes for two nonsteroidal anti‐inflammatory drugs in various real samples. Characterization techniques, such as IR, X‐ray powder diffraction, field emission scanning electron microscopy, energy‐dispersive X‐ray‐mapping, and Brunauer‐Emmett‐Teller (BET), were used to confirm the correctness synthesis and preparation of the nanocomposites. Effective parameters on the extraction efficiency were investigated to maximize the analytical performance of the developed method. The dynamic range (1–1000 µg L−1), coefficients of determination (R2 ≥ 0.997), the limits of detection (0.3–1.0 µg L−1), and limit of quantification (1.0–3.0 µg L−1), intra‐day and inter‐day precisions (3.5%–7.2%) were achieved. The method validation results showed extraction recovery ranging from 80.4% to 96.0% and preconcentration factors ranging from 137 to 140.
Differentiation of five forensically relevant body fluids using a small set of microRNA markers
Authors: Altmeyer Linus , Baumer Karine , Hall Diana 
Published: July 2024

AbstractIn forensic investigations, identifying the type of body fluid allows for the interpretation of biological evidence at the activity level. Over the past two decades, significant research efforts have focused on developing molecular methods for this purpose. MicroRNAs (miRNAs) hold great promise due to their tissue‐specific expression, abundance, lack of splice variants, and relative stability. Although initial findings are promising, achieving consistent results across studies is still challenging, underscoring the necessity for both original and replication studies. To address this, we selected 18 miRNA candidates and tested them on 6 body fluids commonly encountered in forensic cases: peripheral blood, menstrual blood, saliva, semen, vaginal secretion, and skin. Using reverse transcription quantitative PCR analysis, we confirmed eight miRNA candidates (miR‐144‐3p, miR‐451a, miR‐205‐5p, miR‐214‐3p, miR‐888‐5p, miR‐891a‐5p, miR‐193b‐3p, miR‐1260b) with high tissue specificity and four (miR‐203a‐3p, miR‐141‐3p, miR‐200b‐3p, miR‐4286) with lesser discrimination ability but still contributing to body fluid differentiation. Through principal component analysis and hierarchical clustering, the set of 12 miRNAs successfully distinguished all body fluids, including the challenging discrimination of blood from menstrual blood and saliva from vaginal secretion. In conclusion, our results provide additional data supporting the use of a small set of miRNAs for predicting common body fluids in forensic contexts. Large population data need to be gathered to develop a body fluid prediction model and assess its accuracy.
Earth Surface Exchanges (ESEX) Response to the discussion ‘Swelling and flow of expanding clays as a cause for non‐tectonic deformations in a glacial–interglacial environment: Holy Cross Mountains, Poland’ Earth Surface Processes and Landforms 2023: 1–16. DOI: 10.1002/esp.5609—reply
Authors: Jurewicz Edyta , Karnkowski Paweł , Czarnecka‐Skwarek Anna , Wójcik Emilia , Gawriuczenkow Ireneusz 
Published: July 2024

AbstractIn response to the revision of our article on the nontectonic origins of unusually steep and overturned strata in the Mesozoic of the Holy Cross Mountains (HCM), we refute arguments that the authors of discussion make our theory implausible. The authors focused on proving the presence of strike‐slip faults in the HCM, but at the same time, they believe that fold deformations precede the faults that cut them. The explanations provided by the critics still do not explain the extraordinary convergence of the morphology, surface of the fault and deformations occurring in its vicinity over a distance of 27 km. This problem was solved in our previous study, in which we have suggested that the steep and overturned orientation of the Oxfordian strata may be the result of non‐tectonic processes. We consider the arguments used by the critics to be incorrect.
Adenosine A2B receptors differently modulate oligodendrogliogenesis and myelination depending on their cellular localization
Authors: Cherchi Federica , Venturini Martina , Magni Giada , Frulloni Lucia , Chieca Martina , Buonvicino Daniela , Santalmasi Clara , Rossi Francesca , De Logu Francesco , Coppi Elisabetta , Pugliese Anna Maria 
Published: July 2024

AbstractDifferentiation of oligodendrocyte precursor cells (OPCs) into mature oligodendrocytes (OLs) is a key event for axonal myelination in the brain; this process fails during demyelinating pathologies. Adenosine is emerging as an important player in oligodendrogliogenesis, by activating its metabotropic receptors (A1R, A2AR, A2BR, and A3R). We previously demonstrated that the Gs‐coupled A2BR reduced differentiation of primary OPC cultures by inhibiting delayed rectifier (IK) as well as transient (IA) outward K+ currents. To deepen the unclear role of this receptor subtype in neuron‐OL interplay and in myelination process, we tested the effects of different A2BR ligands in a dorsal root ganglion neuron (DRGN)/OPC cocultures, a corroborated in vitro myelination assay. The A2BR agonist, BAY60‐6583, significantly reduced myelin basic protein levels but simultaneously increased myelination index in DRGN/OPC cocultures analyzed by confocal microscopy. The last effect was prevented by the selective A2BR antagonists, PSB‐603 and MRS1706. To clarify this unexpected data, we wondered whether A2BRs could play a functional role on DRGNs. We first demonstrated, by immunocytochemistry, that primary DRGN monoculture expressed A2BRs. Their selective activation by BAY60‐6583 enhanced DRGN excitability, as demonstrated by increased action potential firing, decreased rheobase and depolarized resting membrane potential and were prevented by PSB‐603. Throughout this A2BR‐dependent enhancement of neuronal activity, DRGNs could release factors to facilitate myelination processes. Finally, silencing A2BR in DRGNs alone prevents the increased myelination induced by BAY60‐6583 in cocultures. In conclusion, our data suggest a different role of A2BR during oligodendrogliogenesis and myelination, depending on their activation on neurons or oligodendroglial cells.
Surgical mentorship in low‐resource environments: Opportunities and challenges, a perspective
Authors: Awuah Wireko A. , Tan Joecelyn K. , Bharadwaj Hareesha R. , Aderinto Nicholas , Ferreira Tomas , Patel Heli , Shah Muhammad H. , Kapoor Abhay A. , Banerjee Sumitaksha , Abdul‐Rahman Toufik , Atallah Oday 
Published: July 2024

AbstractBackground and aimsIn low‐ and middle‐income countries (LMICs), a shortage of skilled surgical practitioners hampers healthcare delivery, impacting well‐being and economic growth. Surgical mentorship programs offer a promising solution but face challenges in implementation. This review aims to comprehensively assess the impact of surgical mentorship programs in LMICs and identify challenges and opportunities for their development and implementation.MethodsA thorough literature search was conducted from 2000 to 2023 using multiple databases, focusing on surgical mentorship programs in LMICs. Inclusion criteria encompassed full‐text articles in English that demonstrated characteristics of mentorship. Rigorous exclusion criteria were applied to ensure high‐quality evidence inclusion.ResultsSurgical mentorship programs in LMICs strengthen local surgical capacity, improve surgical skills and patient outcomes, optimize resources and technology utilization, and positively impact medical students aspiring to be surgeons. However, challenges such as resistance to change, resource limitations, financial constraints, logistical and technological challenges, and time constraints hinder their implementation.ConclusionDespite challenges, surgical mentorship programs hold promise for enhancing surgical capacity and healthcare quality in LMICs. Standardized metrics for accountability, innovative funding mechanisms, collaborative partnerships for scalability, interdisciplinary integration, and leveraging virtual mentorship programs are key strategies to overcome challenges and foster sustainable learning cultures, ultimately contributing to improved healthcare equity and quality in low‐resource settings.
Does vitamin D supplementation impact serotonin levels? A systematic review and meta‐analysis
Authors: Alimohammadi‐Kamalabadi Malek , Ziaei Somayeh , Hasani Motahareh , Mohammadi Shooka , Mehrbod Milad , Morvaridi Mehrnaz , Persad Emma , Belančić Andrej , Malekahmadi Mahsa , Estêvão Maria Dulce da Mota Antunes de Oliveira , Daneshzad Elnaz , Heshmati Javad 
Published: July 2024

AbstractBackground and AimsVitamin D deficiency impacts a significant proportion of the world's population, and this deficiency has been linked to various conditions characterized by imbalanced serotonin regulation. The objective of this systematic review and meta‐analysis was to evaluate the effect of vitamin D supplementation on serum serotonin levels.MethodsWe conducted a comprehensive search of PubMed, Scopus, Cochrane Central for Randomized Clinical Trials, and Web of Science up to September 2022, without any language restrictions. The effect sizes were calculated using the standard mean difference (SMD) and 95% confidence interval (CI).ResultsSix randomized clinical trials involving 356 participants were included in the analysis. Our findings indicated no significant changes in serotonin levels between the intervention and control groups (SMD: 0.24 ng/mL, 95% CI: −0.28, 0.75, p > 0.10). Subgroup analysis also did not reveal any significant changes in serotonin levels among children, participants with autism spectrum disorders, interventions lasting 10 weeks or longer, or those receiving vitamin D doses below 4000 IU/day.ConclusionAlthough the results obtained in this systematic review are inconclusive, they support the need for further well‐designed randomized trials to assess the potential role of vitamin D supplementation in regulating serotonin levels and potentially ameliorating depression and related disorders.
Enhanced expression of galectin‐9 in triple negative breast cancer cells following radiotherapy: Implications for targeted therapy
Authors: Lerévérend Cédric , Kotaich Nour , Cartier Lucille , De Boni Manon , Lahire Sarah , Fichel Caroline , Thiebault Charlotte , Brabencova Eva , Maquin Célia , Barbosa Elodie , Corsois Laurent , Hotton Judicael , Guendouzen Sofiane , Guilbert Philippe , Lepagnol‐Bestel Aude‐Marie , Cahen‐Doidy Laurence , Lehmann‐Che Jacqueline , Devy Jérôme , Bensussan Armand , Le Jan Sébastien , Pommier Arnaud , Merrouche Yacine , Le Naour Richard , Vignot Stéphane , Potteaux Stephane 
Published: July 2024

AbstractOptimizations are expected in the development of immunotherapy for the treatment of Triple‐negative breast cancer (TNBC). We studied the expression of galectin‐9 (Gal‐9) after irradiation and assessed the differential impacts of its targeting with or without radiotherapy. Tumor resections from TNBC patients who received neoadjuvant radiotherapy revealed higher levels of Gal‐9 in comparison to their baseline level, only in non‐responder patients. Gal‐9 expression was also found to be increased in TNBC tumor biopsies and cell lines after irradiation. We investigated the therapeutic advantage of targeting Gal‐9 after radiotherapy in mice. Irradiated 4T1 cells or control non‐irradiated 4T1 cells were injected into BALB/c mice. Anti‐Gal‐9 antibody treatment decreased tumor progression only in mice injected with irradiated 4T1 cells. This proof‐of‐concept study demonstrates that Gal‐9 could be considered as a dynamic biomarker after radiotherapy for TNBC and suggests that Gal‐9 induced‐overexpression could represent an opportunity to develop new therapeutic strategies for TNBC patients.
A case with an ectopic ejaculatory duct opening into the bladder trigone in Zinner syndrome, congenital unilateral renal agenesis, and an ipsilateral seminal vesicle cyst
Authors: Hyuga Taiju , Tanabe Kazuya , Kubo Taro , Moriya Kimihiko 
Published: July 2024

IntroductionThis report describes a case with an ectopic ejaculatory duct opening into the bladder trigone in Zinner syndrome, congenital unilateral renal agenesis, and an ipsilateral seminal vesicle cyst.Case presentationThe patient was identified when no left kidney was detected in the fetal period. Abdominal ultrasonography and pelvic plain MRI at 6 months old revealed a 10‐mm cystic lesion on the dorsal aspect of the bladder. Cysto‐urethroscopy at 1 year old revealed a rather short posterior urethra and right and left inferior crests extending from the posterior urethra beyond the bladder neck. The ejaculatory duct opening was identified on the bladder trigone.ConclusionAnatomical abnormality of the ejaculatory duct may represent a cause of infertility and ejaculatory dysfunction in Zinner syndrome. Endoscopic evaluation should be performed for this rare anomaly, even in children.