bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three‐dimensional pore structure of the decellularized parsley scaffold regulates myogenic differentiation for cell cultured meat
Authors: Chen Ziying , Xiong Wanli , Guo Yuzhe , Jin Xin , Wang Lu , Ge Chengxin , Tan Wensong , Zhou Yan 
Published: July 2024

AbstractDecellularized plant scaffolds have been used to develop edible scaffolds for cell cultured meat because of their natural structures similar to that of mammalian tissues. However, their diverse three‐dimensional (3D) porous structures may lead to differences in myogenic differentiation of skeletal muscle cells. In this study, parsley plant tissues were decellularized and modified by type A gelatin and transglutaminase while retaining, respectively, longitudinal fibrous and transverse honeycomb pore structures. The effects of the structure of the decellularized parsley scaffold on the proliferation and myogenic differentiation of C2C12 cells were investigated and the quality of cell cultured meat was evaluated. The results showed that fibrous pore structure guided cells to be arranged in parallel, whereas honeycomb pore structure connected cells in a circular pattern. After induced differentiation, the fibrous scaffolds were more inclined to form multinucleated myotubes with higher expression of myogenic genes and proteins, and the final cell‐based meat contained higher total protein content. Decellularized plant scaffolds with fibrous pore structure were more suitable for myogenic differentiation of C2C12 cells, providing support to the development of edible scaffolds for cultured meat.Practical ApplicationThis study investigated the different three‐dimensional (3D) pore structure of parsley parenchyma to gain insight into how the 3D pore structure of decellularized plant scaffolds regulates myogenic differentiation, which is expected to address the unstable myogenic differentiation of skeletal muscle cells on decellularized plant scaffolds in cell culture meat production.
Custom‐printed microfluidic chips using simultaneous ratiometric fluorescence with “Green” carbon dots for detection of multiple antibiotic residues in pork and water samples
Authors: Yang Wenming , Cao Lingling , Lu Hongjie , Huang Yao , Yang Wenqi , Cai Yuanzheng , Li Sumin , Li Shuqi , Zhao Jianwen , Xu Wanzhen 
Published: July 2024

AbstractIn the evolving field of food safety, rapid and precise detection of antibiotic residues is crucial. This study aimed to tackle this challenge by integrating advanced inkjet printing technology with sophisticated microfluidic paper‐based analytical devices (µPADs). The µPAD design utilized “green” quantum dots synthesized via an eco‐friendly hydrothermal method using green white mulberry leaves as the carbon source, serving as the key fluorescent detection material. The action mechanism involved a photoinduced electron transfer system using red carbon dots (CDs) as electron donors and blue CDs combined with two‐dimensional layered molybdenum disulfide (MoS2) nanosheets as electron acceptors. This system could quickly detect antibiotics within 10 min in pork and water samples, demonstrating high sensitivity and recovery rates: 6.5 pmol/L at 99.75%–110% for sulfadimethoxine, 3.3 pmol/L at 99%–105% for sulfamethoxazole, and 8.5 pmol/L at 98.5%–105% for tetracycline. It achieved a relative standard deviation under 5%, ensuring reliability and reproducibility. The fabricated sensor offered a promising application for the rapid and efficient on‐site detection of antibiotic residues in food.
Pulsed light treatment of whole white button mushroom (Agaricus bisporus): Kinetics and mechanism of microbial inactivation and storage study
Authors: Dhawan Anshul , Chakraborty Snehasis 
Published: July 2024

AbstractThe whole white button mushrooms (WWBMs) are highly perishable due to susceptibility to microbial spoilage. This study explored the potential of pulsed light (PL) treatment for decontamination and shelf‐life extension of WWBM. WWBM surface was inoculated with Escherichia coli, Listeria monocytogenes, and Aspergillus niger spores (8.1, 8.0, and 8.05 log10 CFU/g, respectively) and tested for inactivation against various PL intensities (fluence 0.13–0.75 J/cm2). The kinetics and mechanism of microbial inactivation were explored, and shelf life was determined at 4, 20, and 37°C. Microbial inactivation increased with increasing PL intensity. PL‐induced microbial inactivation was well explained by Weibull model with shape parameters (β‐value) for E. coli, L. monocytogenes, A. niger, aerobic mesophiles, and yeast and mold as 0.87, 0.92, 0.91, 0.89, and 0.94, respectively. PL‐treatment at 0.75 J/cm2 resulted in >5‐log cycle reduction in all inoculated and natural microorganisms. Exposure to PL led to collapse of cellular structure, ruptured cell wall, and leakage of cellular material in all microorganisms and spores along with alterations in nucleic acid and lipid bands. At 4°C, maximum shelf life of 5 days was achieved when WWBM was exposed at 0.75 J/cm2. The WWBM retained 83.3% phenolics, 83.9% antioxidant capacity, and 77.4% vitamin D2 at 4°C while reducing the polyphenol oxidase and peroxidase activity by 89% and 79%. The degradation rate for quality parameters increased with storage temperature. The activation energy of the browning index affirmed it as the most sensitive quality attribute during storage. The study concluded the potential of PL treatment to prolong the shelf life of WWBM.
Elucidating dietary secrets of the Blue‐headed Macaw, Primolius couloni (Sclater, 1876), through citizen‐sourced photographs
Authors: Brito Lucilene , Cavalcante Angela de Souza , Santos Lucas Sobral , Campioto Ana Laura , Silva Paulo Antonio 
Published: July 2024

AbstractDietary data are vital for understanding species' resource requirements and ecological roles and supporting conservation efforts. The Blue‐headed Macaw, Primolius couloni, is a vulnerable and endemic species in the Amazon. Due to their rarity, elusive nature, complex forest canopy habitat, and occurrence in remote areas, we minimally know their dietary habits. To address this gap, we analysed photographs of Blue‐headed Macaws posted on wildlife‐focused social media platforms, specifically targeting images that capture foraging behaviour on plants. Photographs depicting the same foraging were included only once, and those where the plants were not identified were discarded. By doing so, we identified 36 distinct foraging events (or photos), primarily taken between July and October, concentrated in specific locations in Peru and Brazil, a potential seasonality in foraging. We documented 27 food plant species and items such as nectar (n = 9 photos), pulp (8), seeds (7), buds, bark (5 each), aril, and leaves (n = 1 photo each). Notably, floral resources, including nectar, are a potentially important food in the driest months. Using Levin's Index (Ba), we identified a broad dietary niche, indicating a generalist feeding strategy with a wide variety of plant species (Ba = 0.69) and food items (Ba = 0.88). Additionally, the study revealed interactions such as florivory, seed predation, bark consumption, and potential mutualistic roles like pollination and seed dispersal. Our photo‐centric approach here expanded the known dietary spectrum of the Blue‐headed Macaw from four to 31 plant species, offering new insights for conservation strategies. We recommend the preservation of identified food plants and suggest using these data to inform habitat restoration efforts that support the macaws' dietary needs and ecological functions. Future research should focus on continuous monitoring of these food plants to deepen our understanding of seasonal foraging trends and ecological interactions and refine conservation approaches.
Enhancing invasive alien plant eradication outcomes: Lessons learned from South Africa
Authors: Nsikani Mlungele M. , Geerts Sjirk 
Published: July 2024

AbstractSeveral attempts to eradicate invasive alien species have been initiated in South Africa and despite no formal eradication declaration, several species have been brought down to low levels or are now absent from the country. Based on an expert workshop with South African implementing agencies on best nationwide eradication practices, we recommend that eradication programmes should be well coordinated, and characterised by collaboration between implementing agencies, researchers and practitioners. Countries should have a list of eradication targets informed by the best available science, with targets ranked according to priority. The best eradication model should be used consistently until eradication is achieved. While most of these priority areas are not novel, it does provide a perspective from a biodiverse African country with limited funding, based on 16 years of experience in implementing eradication programmes. It corroborates challenges experienced elsewhere in the world and contributes to enhancing eradication outcomes globally.
Surgical outcomes in ileal Crohn's disease complicated by ileosigmoid fistula
Authors: Jenkin Ashley , Edmundson Aleksandra , Clark David 
Published: July 2024

AbstractBackgroundThe management of Crohn's disease (CD) complicated by ileosigmoid fistula (ISF) remains a challenge, and Australian outcomes have not previously been reported.MethodsA retrospective review of a tertiary colorectal inflammatory bowel disease unit, across public and private sites, from 2005 to 2023 to identify adult patients having undergone operative management of ISF.ResultsTwenty‐nine patients underwent surgery for ISF in the study period. Seventeen were male and the median age was 40 years. The pre‐operative diagnosis of ISF was made in 76%, and patients were more likely to undergo resectional surgery if the pre‐operative diagnosis was made endoscopically. Sixty‐nine percent of cases were performed electively, with 76% completed laparoscopically with an 18.5% conversion rate to an open approach. The ISF was oversewn in three patients, repaired primarily in 14 patients, managed with segmental wedge resection in eight patients and resected via an anterior resection in four patients. The rate of stoma formation at the index procedure was 20.7% overall and 22% in patients being acutely managed with steroids. Emergent cases were more likely to be defunctioned with a stoma. Morbidity was 17.2% with a single anastomotic leak.ConclusionISF in CD remains difficult to diagnose pre‐operatively, but can be safely managed laparoscopically without formal resection, and with limited use of diverting stoma formation. The specific surgical approach to the sigmoid in ISF is difficult to pre‐determine and often requires decisions to be made intra‐operatively.
What is the humanistic and ethical value of the “logic of gift” in business relationships? A conceptual approach
Authors: Melé Domènec 
Published: July 2024

AbstractOne conventional view of businesses is to reduce them to mere performers of economic transactions in an exercise of exchange based on the “logic of self‐interest,” and under the criterion do ut des, meaning “I give in order that you may give.” Drawing from personalist philosophy, this article argues that financial and organizational interactions are encounters, relations between persons, not mere economic transactions. Furthermore, people involved in business have the capacity to establish relations of gratuity with others under the criterion do propter alterum, that is, “give to the other as another,” without expecting a return. This criterion is at the core of the “logic of gift.” We argue that giving authentic gifts is intrinsically good, since it contributes to the human flourishing of those who practice this behavior. Implementing the “logic of gift” is also beneficial for the company because it contributes to humanizing the company. In addition, applying the logic of gift will probably bring about trust and social cohesion between people, with positive consequences for business performance, which is consistent with previous findings.
Unlocking narratives: Longitudinal associations between theory of mind and reading comprehension
Authors: Osterhaus Christopher , Lecce Serena , Koerber Susanne 
Published: July 2024

AbstractThis study explores the longitudinal association between Theory of Mind (ToM) and reading comprehension (RC) in middle childhood, focusing on three advanced ToM (AToM) components: social reasoning, reasoning about ambiguity and recognition of social norm transgressions. Over the course of a year, 112 nine‐year‐olds (61 girls, 51 boys; Mage = 9; 0 years, ±4 months at wave 1) were followed from Grade 3 to Grade 4 and assessed for AToM predictors of Grade‐4 RC. Findings show that only social reasoning predicts RC, independent of general intelligence and prior RC performance. In turn, RC did not predict any AToM component. These findings contribute to understanding cognitive development in educational contexts, emphasizing the significance of AToM, particularly social reasoning, in RC.
Exposure to obinutuzumab does not affect outcomes of SARS‐CoV‐2 infection in vaccinated patients with newly diagnosed advanced‐stage follicular lymphoma
Authors: Pinto A. , Caltagirone M. , Battista M. , Gazzoli G. C. , Patti C. , Pennese E. , De Lorenzo S. , Pavone V. , Merli M. , Chiarenza A. , Gorgone A. G. , Piazza F. , Puccini B. , Noto A. , Arcaini L. , De Filippi R. , Zinzani P. L. , Ferreri A. J. M. , Ladetto M. , Ferrari S. , Gritti G. 
Published: July 2024

SummaryURBAN is a multicentric, ambispective study evaluating the effectiveness and safety of obinutuzumab‐based immuno‐chemotherapy and maintenance in patients with untreated advanced follicular lymphoma (FL). The study began before the COVID‐19 emergency declaration in Italy. It is currently ongoing for follow‐up, and the enrolment timeline encompassed different stages of the pandemic, various vaccination roll‐out phases and prevalence of SARS‐CoV‐2 variants. Outcomes of interest of the present sub‐analysis included SARS‐CoV‐2 infection rates and COVID‐19‐related hospitalizations/deaths. At data cut‐off, 86 (28.8%) and 213 patients (71.2%) were treated before and during/after the COVID‐19 outbreak respectively; 294 (98.3%) completed the induction, 31 (10.4%) completed maintenance and 170 (56.9%) were still on maintenance. Overall, 245 patients (81.9%) received at least one SARS‐CoV‐2 vaccine dose: 13.5%, 31.4% and 55.1% received one, two and three doses respectively. We observed a substantial decrease in COVID‐19‐related mortality rates in pre‐ versus post‐vaccination phases, along with a reduction in COVID‐19‐related outcomes due to the shift from alpha/delta to omicron variant predominance. No differences emerged between patients given maintenance or not, although the schedule was modified in 65% of cases. To our knowledge, URBAN represents the largest dataset of COVID‐19‐related outcomes in FL patients extensively exposed to obinutuzumab. ClinicalTrials.gov identifier: NCT04034056.
China's declining business dynamism
Authors: Cerdeiro Diego A. , Ruane Cian 
Published: July 2024

AbstractWe document five novel facts about the dynamism of Chinese manufacturing firms between 2003 and 2018. We show that: (i) the revenue and capital shares of young firms have declined, (ii) life‐cycle growth of firm revenues and assets has declined, (iii) life‐cycle growth of process efficiency / product quality and investment in intangibles has declined (iv) younger firms have higher capital productivity than older firms, with the gap increasing over time, and (v) the dispersion of capital growth and the responsiveness of capital growth to capital productivity have both declined. Using a simple model, we estimate that the lower life‐cycle productivity growth of young firms reduces manufacturing productivity growth by 0.8 percentage points annually, and worsening allocative efficiency of capital between young and old firms reduced manufacturing total factor productivity by 1.25% between the early 2000s and late 2010s. We find that business dynamism is weaker in provinces where state‐owned enterprises account for a larger share of the capital stock.
Plasma cell‐free DNA methylome‐based liquid biopsy for accurate gastric cancer detection
Authors: Qi Jian , Hong Bo , Wang Shujie , Wang Jingyun , Fang Jinman , Sun Ruifang , Nie Jinfu , Wang Hongzhi 
Published: July 2024

AbstractEarly detection plays a critical role in mitigating mortality rates linked to gastric cancer. However, current clinical screening methods exhibit suboptimal efficacy. Methylation alterations identified from cell‐free DNA (cfDNA) present a promising biomarker for early cancer detection. Our study focused on identifying gastric cancer‐specific markers from cfDNA methylation to facilitate early detection. We enrolled 150 gastric cancer patients and 100 healthy controls in this study, and undertook genome‐wide methylation profiling of cfDNA using cell‐free methylated DNA immunoprecipitation and high‐throughput sequencing. We identified 21 differentially methylated regions (DMRs) between the gastric tumor and nontumor groups using multiple algorithms. Subsequently, using the 21 DMRs, we developed a gastric cancer detection model by random forest algorithm in the discovery set, and validated the model in an independent set. The model was able to accurately discriminate gastric cancer with a sensitivity and specificity of 93.90% and 95.15% in the discovery set, respectively, and 88.38% and 94.23% in the validation set, respectively. These results underscore the efficacy and accuracy of cfDNA‐derived methylation markers in distinguishing early stage gastric cancer. This study highlighted the significance of cfDNA methylation alterations in early gastric cancer detection.
Bone volume and height changes for lateral window sinus floor elevation using two types of deproteinized bovine bone mineral: A retrospective cohort study of 1–4 years
Authors: Xiao Pei , Chen Cong , Shen Xiaoting , Xu Antian , Sharaf Mufeed Ahmed , Lu Hongye , He Fuming 
Published: July 2024

AbstractObjectiveTo compare bone volume and height changes of two types of deproteinized bovine bone mineral (DBBM) for lateral window sinus floor elevation (LSFE) with simultaneous implant placement.Materials and MethodsThis retrospective cohort study involved 72 patients who underwent LSFE using low‐temperature sintered cancellous bone‐derived DBBM (C‐DBBM) or high‐temperature two‐step sintered epiphyseal‐derived DBBM (E‐DBBM). Cone‐beam computed tomography (CBCT) was acquired preoperatively, immediately postoperatively, 6 months and 1–4 years post‐surgery. Bone volume (BV), apical bone height (ABH), endo‐sinus bone gain (ESBG), and crestal bone level (CBL) were evaluated through three‐dimensional fitting and superimposition. Linear mixed models (LMM) were employed to analyze factors influencing the reduction of BV (ΔBV) and ESBG (ΔESBG).ResultsThe E‐DBBM group showed no significant change in BV 1–4 years post‐surgery, while the C‐DBBM group demonstrated a significant reduction (p = .006) with volume stability of 85.86%. Bone height in the E‐DBBM group increased at 6 months and subsequently decreased at 1–4 years (p = .003). In the C‐DBBM group, it decreased at 6 months (p = .014), then further decreased at 1–4 years (p = .001). ΔESBG was lower in the E‐DBBM group than the C‐DBBM group from immediate postoperative to 1–4 years (p = .009). LMM showed graft material type was the primary factor influencing ΔBV (p = .026) and ΔESBG (p = .003).ConclusionsWithin the limitations of this study, both types of DBBM could achieve favorable clinical outcomes. E‐DBBM demonstrated enhanced stability in maintaining bone volume and height.
Countering stereotypes: Exploring the characteristics of Aboriginal Australians who do not drink alcohol in a community representative sample
Authors: Conigrave James H. , Wilson Scott , Conigrave Katherine M. , Perry Jimmy , Hayman Noel , Chikritzhs Tanya N. , Wilson Dan , Zheng Catherine , Weatherall Teagan J. , Lee K. S. Kylie 
Published: July 2024

AbstractIntroductionContrary to stereotypes, Aboriginal and Torres Strait Islander Australians are more likely to abstain from drinking than other Australians. We explored characteristics and experiences of Aboriginal and Torres Strait Islander Australians who do not drink alcohol.MethodWe conducted a cross‐sectional, representative survey of 775 Aboriginal and Torres Strait Islander Australians (16+ years) in remote and urban South Australia. We explore correlates of not drinking alcohol using multi‐level logistic regression. We describe reasons for non‐drinking and harms participants experienced in past 12 months from others' drinking.ResultsNon‐drinking participants were more likely to be older (OR 1.35 [95% CI 1.21, 1.50] per decade) and unemployed (OR 2.72 [95% CI 1.77, 4.20]). Participants who spoke Aboriginal Australian languages at home were three times more likely to be lifetime abstainers from drinking (OR 3.07 [95% CI 1.52, 6.21]). Common reasons for not drinking alcohol were health and family. Most did not report harms from others' alcohol consumption (79.6%, 76.9%, urban and remote respectively). Stress from others' alcohol consumption was the most reported harm by non‐drinkers (14.5% and 23.1%, urban and remote, respectively).Discussion and ConclusionsCulture such as speaking Aboriginal Australian languages might have protective effects that promote abstaining but was rarely explicitly cited as a reason for not drinking. A greater understanding of local values held by people who do not drink alcohol could help inform health messaging and other interventions to reduce alcohol‐related harms. Understanding local reasons for abstaining can help tailor health messaging to suit local contexts.
Echocardiographic evaluation of left atrial volume and comparative analysis to left atrial to aortic root ratio in premature neonates and infants with patent ductus arteriosus
Authors: Karimi Malika R. , Bischoff Adrianne R. , Riley Emily , Zerwic Luke , McNamara Patrick J. , Hyland Rachael M. , Ashwath Ravi 
Published: July 2024

AbstractPurposeLeft atrium to aortic root ratio (LA/Ao) is an echocardiographic marker of hemodynamically significant patent ductus arteriosus (PDA). Since 2‐dimensional measurement of the ratio is geometrically limited, left atrial volume (LAV) which has 3‐dimensional characteristics was investigated. The aim of this study was to determine a correlation between LA/Ao ratio and LAV as well as holodiastolic flow reversal in preterm neonates with and without a PDA.MethodsA retrospective evaluation of neonates with and without PDA was performed. Targeted neonatal echocardiography evaluation of LA/Ao and LAV was measured from parasternal long‐axis view and the apical 4 and 2‐chamber views, respectively. Univariate and linear regression analysis were performed.Results200 patients were included of whom 158 (79.0%) had a PDA shunt. The median gestational age at the time of echo was 27.4 weeks (IQR: 25.7–29.4 weeks). The median LA/Ao ratio was 1.51 (IQR: 1.26–1.83) and median LAV indexed to weight was .91 mL/kg (IQR: .65–1.18 mL/kg). There was a significant correlation between LA/Ao and LAV indexed to weight in the PDA group (r2 = .080, p = .0003). LA/Ao ratio and LAV indexed to weight differed significantly between those with diastolic flow reversal versus no‐flow reversal (LA/Ao, p = .003; LAV, p = .001).ConclusionsThis study demonstrated a significant correlation between LA/Ao and LAV in preterm infants with PDA, with greater magnitude of discordance for LAV. The power of LAV versus LA/Ao in monitoring hemodynamically significant PDA requires prospective evaluation.