bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aktivitas Antibakteri Sediaan Sirup Ekstrak Daun Sirih Merah (Piper crocotum Ruiz & Pav) Terhadap Bakteri Salmonella typhii
Authors: Damayanti Linda Meydigret , Mahdiyah Dede , Noval Noval , Nastiti Kunti 
Published: July 2024

Demam tifoid merupakan penyakit menular yang tersebar di seluruh dunia dan sampai sekarang masih menjadi masalah kesehatan terbesar di negara berkembang dan tropis. Insiden penyakit ini masih sangat tinggi dengan lebih dari 700 kasus berakhir dengan kematian. Bakteri Salmonella typhii masuk melalui mulut ke dalam saluran pencernaan. Daun sirih merah (Piper crocatum) memiliki senyawa kimia yang bermanfaat antara lain alkaloid, saponin, tanin, dan flavonoid yang memiliki sifat antibakteri dan antiinflamasi. Uji aktivitas antibakteri banyak dilakukan langsung pada ekstrak tumbuhan, belum ada uji pada bentuk sediaan seperti larutan sirup. Sirup juga sediaan cair yang dapat digunakan oleh hampir semua usia, cepat diabsorpsi, sehingga cepat menimbulkan efek. Tujuan penelitian ini untuk menguji aktivitas dari sediaan sirup ekstrak daun sirih merah (Piper crocatum Ruiz & Pav) terhadap bakteri Salmonella typhii. Penelitian ini menggunakan metode true experimental dengan rancangan post test only with control group design. Sampel yang digunakan pada penelitian ini adalah sirup ekstrak daun sirih merah konsentrasi 5 %, 10 % dan 15 %. Evaluasi sirup meliputi uji organoleptis, homogenitas, pH. Kontrol positif yang digunakan adalah kloramfenikol dan kontrol negatif yang digunakan adalah sediaan sirup tanpa ekstrak. Sediaan sirup ekstrak daun sirih merah memiliki diameter zona hambat sebesar 32,19 mm. KHM pada konsentrasi 10% dengan nilai signifikansi 0,018 namun tidak memiliki nilai KBM. Sediaan sirup ekstrak daun sirih merah telah memenuhi evaluasi organoleptis, homogenitas, pH. Sirup ekstrak Daun Sirih Merah (Piper crocatum Ruiz & Pav) memiliki daya hambat minimum terhadap Salmonella typhii pada konsentrasi 10%.
Comparação dos resultados da análise de folhas de café acondicionadas em diferentes tipos de embalagem
Authors: Silva Assis Brian Gabriel , Amaral Priscila Ferreira de Sales , Amaral Francisco Hélcio Canuto 
Published: July 2024

Este trabalho teve como objetivo avaliar se houve diferença nos resultados da análise de folhas de café acondicionadas em embalagens de papel e de plástico (com furo e sem furo). Buscou-se ainda verificar se o tempo em que análise foliar foi realizada (oitavo dia e décimo quinto dia após a coleta) seguiu a mesma tendência observada para as amostras que foram coletadas e imediatamente analisadas. Para atingir o objetivo proposto, as folhas das plantas foram inicialmente recolhidas em uma fazenda localizada na região de Boa Esperança e então conduzidas ao laboratório para serem realizadas as análises. Avaliou-se os resultados por meio do emprego do Reconhecimento de Padrões não-supervisionado composto pela Análise de Agrupamentos Hierárquicos (HCA) e pela Análise de Componentes Principais (PCA). Foi verificado que independentemente do tipo de embalagem em que as folhas de café foram acondicionadas, há uma elevada similaridade entre as amostras analisadas no dia da coleta e no oitavo dia após a coleta, as quais, por sua vez, são distintas das avaliadas no décimo quinto dia após a coleta. Tais resultados permitem inferir que a influência do tempo após a coleta se sobrepõe à influência do tipo de embalagem. Sendo assim, pode-se concluir que as folhas de café podem ser analisadas até o oitavo dia após serem coletadas, sendo acondicionadas em qualquer tipo de embalagem estudada (papel, plástico com furo e plástico sem furo).
Novel PRKAR1A mutation in Carney complex: a case report and literature review
Authors: Zheng Huaqiang , Kang Hong , Qiu Yizhen , Xie Liangxiao , Wu Jinzhi , Lai Pengbin , Kang Jiapeng 
Published: July 2024

ObjectiveCarney complex is a rare autosomal dominant syndrome that has been shown to be associated with inactivation due to PRKAR1A mutations. We revealed a novel PRKAR1A gene mutation in Chinese patient with Carney complex and review the literature to enhance understanding of Carney complex.Case presentationA 23-year-old Chinese male patient with a family history cardiac myxoma was admitted to our Department of Endocrinology because of central obesity and hyperpigmentation. Physical examination revealed a maximum blood pressure of 150/93mmHg, a waist circumference of 102cm, a weight of 70kg, a height of 170cm, and a BMI of 24.22kg/m2. Additionally, there was spotty skin pigmentation on the lip mucosa, purple striae on the abdomen, thin skin on both legs, and visible veins. Blood examination revealed hypercortisolemia, decreased adrenocorticotropic hormone (ACTH) levels and failure to suppress cortisol with low and high-dose dexamethasone suppression tests. Magnetic resonance imaging (MRI) scan revealed multiple small adrenal nodules and Retroperitoneal neurogenic tumor. Genetic testing showed a novel heterozygous mutation in exon 5 of PRKAR1A (c.500_502 + 8delAAGGTAAGGGC). The patient underwent resection of the right adrenal gland and retroperitoneal neoplasms in 2020. Postoperative pathology following the right adrenal gland resection showed nodular hyperplasia of the adrenal cortex. The pathology from the retroperitoneal tumor resection revealed spindle cell tumors rich in pigment and cells. The patient was diagnosed as Carney complex according to Stratakis CA in 2001 guidelines. After long-term follow-up, the patient’s condition was stable, with weight loss, waist circumference reduction, significantly lower cortisol levels, and normal blood lipids.ConclusionThis case reported a Carney complex in a Chinese patient, characterized clinically by non-ACTH-dependent Cushing’s syndrome, familial recurrent cardiac myxomas, psammomatous melanotic schwannoma (PMS) and skin and mucosal pigmentation. A novel subtype of PRKAR1A mutation was discovered, which may affect the characteristics of the PRKAR1A protein and contribute to the development of Carney complex.
Association between fatty acids intake and bone mineral density in adolescents aged 12-19: NHANES 2011–2018
Authors: Wang Zhi-Gang , Fang Ze-Bin , Xie Xiao-Li 
Published: July 2024

BackgroundThe relationship between the intake of dietary fatty acids (FA) and bone mineral density (BMD) has been the subject of prior investigations. However, the outcomes of these studies remain contentious. The objective of this research is to examine the link between dietary FA consumption among adolescents and BMD.MethodsThis study utilized high-quality data from the National Health and Nutrition Examination Survey database, spanning 2011 to 2018, to explore the association between dietary fatty acids and bone health indicators in adolescents, including BMD and bone mineral content (BMC). Analyses were performed using weighted multivariate linear regression models, incorporating detailed subgroup analysis.ResultsThe study included 3440 participants. Analysis demonstrated that intake of saturated fatty acids (SFA) was positively correlated with total BMD, left arm BMD, total BMC, and left arm BMC. Monounsaturated fatty acid (MUFA) intake was positively correlated with BMC across most body parts, though it showed no correlation with BMD. Intake of polyunsaturated fatty acids (PUFA) was significantly inversely correlated with both BMD and BMC in most body parts. Additionally, subgroup analysis indicated that variables such as sex, age, standing height, and race significantly influenced the correlation between FA intake and BMD.ConclusionsOur study indicates that dietary intake of SFA may benefit to BMD in adolescents, in contrast to PUFA and MUFA. Therefore, we recommend that adolescents maintain a balanced intake of SFA to promote optimal bone mass development while preserving metabolic health.
Relationships between systemic sclerosis and atherosclerosis: screening for mitochondria-related biomarkers
Authors: Wang Fei , Lyu Xiao Yan , Qin Yi Ming , Xie Mei Juan 
Published: July 2024

BackgroundPatients with systemic sclerosis (SSc) are known to have higher incidence of atherosclerosis (AS). Mitochondrial injuries in SSc can cause endothelial dysfunction, leading to AS; thus, mitochondria appear to be hubs linking SSc to AS. This study aimed to identify the mitochondria-related biomarkers of SSc and AS.MethodsWe identified common differentially expressed genes (DEGs) in the SSc (GSE58095) and AS (GSE100927) datasets of the Gene Expression Omnibus (GEO) database. Considering the intersection between genes with identical expression trends and mitochondrial genes, we used the least absolute shrinkage and selection operator (LASSO) as well as random forest (RF) algorithms to identify four mitochondria-related hub genes. Diagnostic nomograms were then constructed to predict the likelihood of SSc and AS. Next, we used the CIBERSORT algorithm to evaluate immune infiltration in both disorders, predicted the transcription factors for the hub genes, and validated these genes for the two datasets.ResultsA total of 112 genes and 13 mitochondria-related genes were identified; these genes were then significantly enriched for macrophage differentiation, collagen-containing extracellular matrix, collagen binding, antigen processing and presentation, leukocyte transendothelial migration, and apoptosis. Four mitochondria-related hub DEGs (IFI6, FSCN1, GAL, and SGCA) were also identified. The nomograms showed good diagnostic values for GSE58095 (area under the curve (AUC) = 0.903) and GSE100927 (AUC = 0.904). Further, memory B cells, γδT cells, M0 macrophages, and activated mast cells were significantly higher in AS, while the resting memory CD4+ T cells were lower and M1 macrophages were higher in SSc; all of these were closely linked to multiple immune cells. Gene set enrichment analysis (GSEA) showed that IFI6 and FSCN1 were involved in immune-related pathways in both AS and SSc; GAL and SGCA are related to mitochondrial metabolism pathways in both SSc and AS. Twenty transcription factors (TFs) were predicted, where two TFs, namely BRCA1 and PPARγ, were highly expressed in both SSc and AS.ConclusionFour mitochondria-related biomarkers were identified in both SSc and AS, which have high diagnostic value and are associated with immune cell infiltration in both disorders. Hence, this study provides new insights into the pathological mechanisms underlying SSc and AS. The specific roles and action mechanisms of these genes require further clinical validation in SSc patients with AS.
Novel pathogenic variants of DNAH5 associated with clinical and genetic spectra of primary ciliary dyskinesia in an Arab population
Authors: Al-Mutairi Dalal A. , Alsabah Basel H. , Pennekamp Petra , Omran Heymut 
Published: July 2024

Introduction: Primary ciliary dyskinesia (PCD) is caused by the dysfunction of motile cilia resulting in insufficient mucociliary clearance of the lungs. This study aimed to map novel PCD variants and determine their pathogenicity in PCD patients in Kuwait.Methods: Herein, we present five PCD individuals belonging to a cohort of 105 PCD individuals recruited from different hospitals in Kuwait. Genomic DNAs from the family members were analysed to screen for pathogenic PCD variants. Transmission electron microscopy (TEM) and immunofluorescence (IF) analyses were performed on the nasal biopsies to detect specific structural abnormalities within the ciliated cells.Results: Genetic screening and functional analyses confirmed that the five PCD individuals carried novel pathogenic variants of DNAH5 causing PCD in three Arabic families. Of these, one multiplex family with two affected individuals showed two novel homozygous missense variants in DNAH5 causing PCD with situs inversus; another multiplex family with two affected individuals showed two newly identified compound heterozygous variants in DNAH5 causing PCD with situs solitus. In addition, novel heterozygous variants were identified in a child with PCD and situs solitus from a singleton family with unrelated parents. TEM analysis demonstrated the lack of outer dynein arms (ODAs) in all analysed samples, and IF analysis confirmed the absence of the dynein arm component of DNAH5 from the ciliary axoneme.Conclusion: The newly identified pathogenic variants of DNAH5 are associated with PCD as well as variable pulmonary clinical manifestations in Arabic families.
The causal relationship between gut microbiota and nine infectious diseases: a two-sample Mendelian randomization analysis
Authors: Wang Song , Yin Fangxu , Sun Wei , Li Rui , Guo Zheng , Wang Yuchao , Zhang Yiyuan , Sun Chao , Sun Daqing 
Published: July 2024

BackgroundEvidence from observational studies and clinical trials has associated gut microbiota with infectious diseases. However, the causal relationship between gut microbiota and infectious diseases remains unclear.MethodsWe identified gut microbiota based on phylum, class, order, family, and genus classifications, and obtained infectious disease datasets from the IEU OpenGWAS database. The two-sample Mendelian Randomization (MR) analysis was then performed to determine whether the gut microbiota were causally associated with different infectious diseases. In addition, we performed reverse MR analysis to test for causality.ResultsHerein, we characterized causal relationships between genetic predispositions in the gut microbiota and nine infectious diseases. Eight strong associations were found between genetic predisposition in the gut microbiota and infectious diseases. Specifically, the abundance of class Coriobacteriia, order Coriobacteriales, and family Coriobacteriaceae was found to be positively associated with the risk of lower respiratory tract infections (LRTIs). On the other hand, family Acidaminococcaceae, genus Clostridiumsensustricto1, and class Bacilli were positively associated with the risk of endocarditis, cellulitis, and osteomyelitis, respectively. We also discovered that the abundance of class Lentisphaeria and order Victivallales lowered the risk of sepsis.ConclusionThrough MR analysis, we found that gut microbiota were causally associated with infectious diseases. This finding offers new insights into the microbe-mediated infection mechanisms for further clinical research.
T-cell responses in colorectal peritoneal metastases are recapitulated in a humanized immune system mouse model
Authors: Saris Job , Bootsma Sanne , Verhoeff Jan , Tuynman Jurriaan B. , Wildenberg Manon E. , Rijnstra Esther Siteur-van , Lenos Kristiaan J. , Garcia Vallejo Juan J. , Vermeulen Louis , Grootjans Joep 
Published: July 2024

BackgroundThe occurrence of peritoneal metastasis (PM) in patients with colorectal cancer (CRC) has a dismal prognosis. There is often limited response to systemic- and immunotherapy, even in microsatellite unstable (MSI) CRC. To overcome therapy resistance, it is critical to understand local immune environment in the peritoneal cavity, and to develop models to study anti-tumor immune responses. Here, we defined the peritoneal immune system (PerIS) in PM-CRC patients and evaluate the pre-clinical potential of a humanized immune system (HIS) mouse model for PM-CRC.MethodsWe studied the human PerIS in PM-CRC patients (n=20; MSS 19/20; 95%) and in healthy controls (n=3). HIS mice (NODscid gamma background; n=18) were generated, followed by intraperitoneal injection of either saline (HIS control; n=3) or human MSS/MSI CRC cell lines HUTU80, MDST8 and HCT116 (HIS-PM, n=15). Immune cells in peritoneal fluid and peritoneal tumors were analyzed using cytometry by time of flight (CyTOF).ResultsThe human and HIS mouse homeostatic PerIS was equally populated by NK cells and CD4+- and CD8+ T cells, however differences were observed in macrophage and B cell abundance. In HIS mice, successful peritoneal engraftment of both MSI and MSS tumors was observed (15/15; 100%). Both in human PM-CRC and in the HIS mouse PM-CRC model, we observed that MSS PM-CRC triggered a CD4+ Treg response in the PerIS, while MSI PM-CRC drives CD8+ TEMs responses.ConclusionIn conclusion, T cell responses in PM-CRC in HIS mice mirror those in human PM-CRC, making this model suitable to study antitumor T cell responses in PM-CRC.
Pemphigoid diseases in patients with end-stage kidney diseases: pathogenesis and treatment
Authors: Yang Liu-Yi-Yi , Wang Yu-Lu , Zuo Ya-Gang 
Published: July 2024

BackgroundPemphigoid diseases constitute a group of autoimmune blistering disorders characterized by subepithelial blistering. The association between pemphigoid diseases and both end-stage kidney disease (ESKD) and its treatment is notable. However, there is limited evidence about the management of pemphigoid diseases in patients with ESKD. This systematic review compiled case reports and relevant studies, summarized the underlying mechanisms of pemphigoid diseases in patients with ESKD, and summarized the efficacy of various therapies.MethodsA systematic search of PubMed and Embase was performed for articles published between 1982 to June 2, 2024.ResultsFifty-three case reports and eight relevant studies were included. Triggers for pemphigoids in patients with ESKD included materials used to treat ESKD, immune dysregulation of patients with ESKD, and rejection of renal allograft. Treatment for these patients included removing triggers, as well as administering of corticosteroids, mycophenolate mofetil (MMF), tetracyclines, rituximab, methotrexate, dapsone, azathioprine, cyclosporine, intravenous immunoglobin (IVIG), plasmapheresis, and Janus kinase inhibitors.ConclusionRemoving triggers is the most effective strategy. Despite their suboptimal efficacy, corticosteroids remain the most commonly used agents in this patient population. MMF, tetracyclines, and rituximab are less used but with benefits. There are significant adverse effects associated with methotrexate treatment. Other treatment may also be beneficial and require further investigation. These findings may enable clinicians to optimize the therapeutic approach for these patients.
Application effect of the online and offline mixed education mode in nursing practice based on the SMCR communication model
Authors: Chen Qian , Jin Yanhong , Zhong Lixia , Li Yingying , Fu Li , Zhang Weiwei , Xu Qiuying 
Published: July 2024

ObjectiveTo explore the application effect of the online and offline mixed teaching mode in nursing practice teaching based on the Source Message Channel Receiver (SMCR) communication model.MethodsBy using the convenience sampling method, 15 nursing students who practiced their internship in the Department of Critical Care Medicine at Beijing Friendship Hospital affiliated to Capital Medical University for 4 weeks, from 1 April 2022 to 31 December 2023, were selected as the experimental group. A total of 16 nursing students who practiced in the same hospital in 2021 were selected as the control group. The control group used traditional teaching mode for teaching, and the experimental group used the online and offline teaching mode for teaching. The theoretical and practical achievements, competency, and teaching satisfaction of the two groups of nursing students were compared and analyzed after the internship.ResultsThe theoretical knowledge, operational performance, competence of the nurses, and satisfaction with the teaching in the experimental group were higher than those of the control group (P &lt; 0.05).ConclusionBased on the SMCR communication model, exploring the online and offline mixed teaching mode plays an important role in the teaching process of nursing practice, which can not only effectively improve the comprehensive performance of nursing students but also help to improve their satisfaction with teaching and has a positive impact on the cultivation of high-quality nursing talents.
Clinical efficacy of CO2 fractional laser combined with compound betamethasone in treating vitiligo and its impact on inflammatory factors
Authors: Zhang Lina , Zhang Jianzhong , Wang Xin , Zhao Zhonglin , Li Zhifeng , Miao Guoying , Lv Chao 
Published: July 2024

ObjectiveTo analyze the clinical efficacy of CO2 fractional laser combined with compound betamethasone in treating vitiligo and its impact on inflammatory factors.MethodsThe clinical treatment effects, levels of inflammatory factors [interleukin-17 (IL-17), interferon-gamma (IFN-γ), interleukin-10 (IL-10)], prognosis regarding repigmentation and relapse, psychological health (satisfaction).Results① Clinical treatment effects: the total effective rate in Group A was 92.73%, Group B was 74.55%, and Group C was 67.27%, with Group A showing significantly higher effectiveness than Groups B and C (p &lt; 0.05). ② Inflammatory factors: prior to treatment, there was no significant difference in IL-17, IFN-γ, and IL-10 levels among the three groups (p &gt; 0.05); after 3 and 6 months of treatment, the levels of IL-17 and IFN-γ decreased significantly while IL-10 levels increased significantly across all three groups, with Group A showing a more pronounced change compared to Groups B and C (p &lt; 0.05). ③ Prognosis regarding repigmentation and relapse: after 3 and 6 months of treatment, Group A exhibited significantly higher repigmentation rates compared to Groups B and C (p &lt; 0.05); in terms of relapse, Group A had a relapse rate of 5.45%, Group B had 21.82%, and Group C had 23.64%, with Group A showing significantly lower relapse rates compared to Groups B and C (p &lt; 0.05). ④ Quality of life and psychological health: at the end of the 6 month follow-up, the quality of life and psychological health of patients in Group A were significantly higher than those in Groups B and C (p &lt; 0.05). ⑤ Occurrence of adverse reactions: the incidence of adverse reactions was 12.73% in Group A, 10.91% in Group B, and 9.09% in Group C, with no significant difference observed among the three groups (p &gt; 0.05).ConclusionThe application of CO2 fractional laser combined with compound betamethasone in vitiligo patients demonstrates significant efficacy. Compared to sole treatment with CO2 fractional laser or compound betamethasone injections, this combined approach further improves the levels of inflammatory factors in vitiligo patients, reduces the risk of relapse, enhances skin repigmentation, improves quality of life, psychological well-being, without increasing the risk of related adverse reactions. This combined approach merits clinical promotion and application.