bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of weight loss on ankle‐brachial index and interartery blood pressures
Authors: Espeland Mark A. , Lewis Cora E. , Bahnson Judy , Knowler William C. , Regensteiner Judith G. , Gaussoin Sarah A. , Beavers Daniel , Johnson Karen C. ,   the Look AHEAD Research Group
Published: October 2013

ObjectiveTo assess whether weight loss improves markers of peripheral artery disease and vascular stenosis.MethodsThe Action for Health in Diabetes randomized clinical trial compared intensive lifestyle intervention (ILI) for weight loss to a control condition of diabetes support and education (DSE) in overweight or obese adults with type 2 diabetes. Annual ankle and brachial blood pressures over four years were used to compute ankle‐brachial indices (ABIs) and to assess interartery blood pressure differences in 5018 participants.ResultsILI, compared to DSE, produced 7.8% (Year 1) to 3.6% (Year 4) greater weight losses. These did not affect prevalence of low (<0.90) ABI (3.60% in DSE versus 3.14% in ILI; P = 0.20) or elevated (>1.40) ABI (7.52% in DSE versus 7.59% in ILI: P = 0.90), but produced smaller mean (SE) maximum interartery systolic blood pressure differences among ankle sites [19.7 (0.2) mmHg for ILI versus 20.6 (0.2) mmHg for DSE (P < 0.001)] and between arms [5.8 (0.1) mmHg for ILI versus 6.1 (0.1) mmHg for DSE (P = 0.01)].ConclusionsFour years of intensive behavioral weight loss intervention did not significantly alter prevalence of abnormal ABI, however, it did reduce differences in systolic blood pressures among arterial sites.
Structural insights into the aggregation behavior of Murraya koenigii miraculin‐like protein below pH 7.5
Authors: Selvakumar Purushotham , Sharma Nidhi , Tomar Prabhat Pratap Singh , Kumar Pravindra , Sharma Ashwani Kumar 
Published: October 2013

ABSTRACTMurraya koenigii miraculin‐like protein (MKMLP) gradually precipitates below pH 7.5. Here, we explore the basis for this aggregation by identifying the aggregation‐prone regions via comparative analysis of crystal structures acquired at several pH values. The prediction of aggregation‐prone regions showed the presence of four short peptides either in beta sheets or loops on surface of the protein. These peptides were distributed in two patches far apart on the surface. Comparison of crystal structures of MKMLP, determined at 2.2 Å resolution in pH 7.0 and 4.6 in the present study and determined at 2.9 Å in pH 8.0 in an earlier reported study, reveal subtle conformational differences resulting in gradual exposure of aggregation‐prone regions. As the pH is lowered, there are alterations in ionic interactions within the protein interactions of the chain with water molecules and exposure of hydrophobic residues. The analysis of symmetry‐related molecular interfaces involving one patch revealed shortening of nonpolar intermolecular contacts as the pH decreased. In particular, a decrease in the intermolecular distance between Trp103 of the aggregation‐prone peptide WFITTG (103–108) unique to MLPs was observed. These results demonstrated that aggregation occurs due to the cumulative effect of the changes in interactions in two aggregation‐prone defined regions. Proteins 2014; 82:830–840. © 2013 Wiley Periodicals, Inc.
The role of vibrations in single‐molecule charge transport: A case study of oligoynes with pyridine anchor groups
Authors: Ballmann Stefan , Hieringer Wolfgang , Härtle Rainer , Coto Pedro B. , Bryce Martin R. , Görling Andreas , Thoss Michael , Weber Heiko B. 
Published: October 2013

AbstractWe report on measurements on molecular wires in single‐molecule junctions. The target is to identify vibrational signatures in the differential conductance spectra, which cannot be resolved for most molecular wires in the resonant transport regime. For the given molecules, we find indeed vibrational sidepeaks, which can be assigned to the longitudinal modes of the string‐like central motif of the molecule by comparison with density functional theory. These data corroborate that oligoyne wires are ideally suited for characterizing vibrational sidepeaks. A current increase as a consequence of vibrational decoherence observed for thiols is, however, not found due to the pyridine anchor group chosen in the present molecules.
DFT and k · p modelling of the phase transitions of lead and tin halide perovskites for photovoltaic cells
Authors: Even Jacky , Pedesseau Laurent , Jancu Jean‐Marc , Katan Claudine 
Published: October 2013

Abstract3D hybrid organic perovskites, CH3NH3PbX3 (X = halogen), have recently been used to strongly improve the efficiency of dye sensitized solar cells (DSSC) leading to a new class of low‐cost mesoscopic solar cells. CsSnI3 perovskite can also be used for hole conduction in DSSC. Density functional theory and GW corrections are used to compare lead and tin hybrid and all‐inorganic perovskites. The room temperature optical absorption is associated to electronic transitions between the spin–orbit split‐off band in the conduction band and the valence band. Spin–orbit coupling is about three times smaller for tin. Moreover, the effective mass of relevant band edge hole states is small (0.17). The high temperature phase sequence of CsSnI3 leading to the room temperature orthorhombic phase and the recently reported phases of CH3NH3MI3 (where M = Pb, Sn) close to the room temperature, are also studied. Tetragonal distortions from the ideal cubic phase are analysed by a k · p perturbation, including spin–orbit effect. In addition, the non‐centrosymmetric phases of CH3NH3MI3 exhibit a splitting of the electronic bands away from the critical point. The present work shows that their physical properties are more similar to conventional semiconductors than to the absorbers used in DSSC. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Extended nonlinear observer normal forms for a class of nonlinear dynamical systems
Authors: Boutat D. 
Published: October 2013

SummaryTransforming a dynamical system by adding auxiliary dynamics is one of the most recent tools to design an observer. Dynamical systems that have the properties of admitting such transformation have been widely studied. Indeed, in the existing literature, several different types of geometrical and analytical characterizations of such dynamical systems have been established. However, one finds only a few examples on this topic. Therefore, the main focus of this paper is to characterize a class of single‐output dynamical systems that can be transformed by means of a change of coordinates into an extended nonlinear observer normal form. Furthermore, this class provides examples of systems that can be transformed adding to them more than one auxiliary dynamics. To our knowledge, examples on this last fact lack in the existing research literature on this topic. Copyright © 2013 John Wiley & Sons, Ltd.
Dehydration of the Sorel Cement Phase 3Mg(OH)2·MgCl2·8H2O studied by in situ Synchrotron X‐ray Powder Diffraction and Thermal Analyses
Authors: Runčevski Tomče , Dinnebier Robert E. , Freyer Daniela 
Published: October 2013

AbstractDehydration is an important process which affects the chemical, physical and mechanical properties of materials. This article describes the thermal dehydration and decomposition of the Sorel cement phase 3Mg(OH)2·MgCl2·8H2O, studied by in situ synchrotron X‐ray powder diffraction and thermal analyses. Attention is paid on the determination of the chemical composition and crystal structure of the lower hydrates, identified as the phases 3Mg(OH)2·MgCl2·5.4H2O and 3Mg(OH)2·MgCl2·4.6H2O. The crystal structure of 3Mg(OH)2·MgCl2·4.6H2O is solved and refined by the Rietveld method and a structural model for the 3Mg(OH)2·MgCl2·5.4H2O phase is given. These phases show statistical distribution of water molecules, hydroxide and chloride anions positioned as ligands on the magnesium octahedra. A structural scheme of the temperature induced transformations in the thermal range from 25 to 500 °C is presented.
K[Al4(PPh2)7PPh]: An AlII Phosphanide / Phosphinidene Intermediate on the Path to AlP Formation
Authors: Mayo Dennis H. , Peng Yang , DeCarlo Samantha , Li Xiang , Lightstone James , Zavalij Peter , Bowen Kit , Schnöckel Hansgeorg , Eichhorn Bryan 
Published: October 2013

AbstractThe reaction of metastable AlCl·(Et2O)n with potassium diphenylphosphanide results in the formation of the aluminum(II) phosphanide / phosphinidene K[Al4(PPh2)7PPh] compound, which is the first reported aluminum(II) phosphinidene complex. The AlII structure results from ligand decomposition and represents the first step in the conversion of AlPPh2 to AlP and biphenyl.
Mono‐ and Di‐Lanthanide Derivatives of 22‐Tungsto‐2‐antimonate(III), [Ln(H2O)4Sb2W21O72(OH)]10– and [Ln2(H2O)8Sb2W20O70)]8–
Authors: Ismail Amal H. , Bassil Bassem S. , Römer Isabella , Kortz Ulrich 
Published: October 2013

AbstractAbstract. The mono‐lanthanide‐containing [Ln(H2O)4Sb2W21O72(OH)]10– (Ln = Yb (1), Lu (2)), and the di‐lanthanide‐containing [Ln2(H2O)8Sb2W20O70)]8– (Ln = Yb (3), Lu (4), Y (5)) have been synthesized in aqueous‐acidic media and characterized in the solid state. Polyanions 1 and 2 were prepared by reaction of Ln3+ ions with [Sb2W22O74(OH)2]12– (Sb2W22), whereas 3–5 were prepared by using the trilacunary 9‐tungstoantimonate(III) [B‐α‐SbW9O33]9– (SbW9). Polyanions 1–5 crystallized as sodium salts in the triclinic system, space group P$\bar{1}$. All compounds were characterized in the solid state by FTIR, single‐crystal XRD, TGA, and elemental analysis. Polyanions 1–5 contain the lacunary [Sb2W20O70]14– unit (Sb2W20), which is coordinated to either a lanthanide and a tungsten ion, leading to a structure with Cs symmetry in polyanions 1 and 2, or to two lanthanide ions, leading to a structure with idealized C2h symmetry, in polyanions 3–5.
Cost-effective production of recombinant human interleukin 24 by lactose induction and a two-step denaturing and one-step refolding method
Authors: Amirzada Muhammad Imran , Yu Minglei , Gong Xiaohai , Chen Yun , Zhu Ruiyu , Lei Jianyong , Jin Jian 
Published: October 2013

Abstract
Recombinant human interleukin 24 (rhIL24) is a member of the interleukin 10 (IL10) family of cytokines with novel therapeutic properties. Human IL24 possesses three N glycosylation sites and a disulfide bridge. The cost and composition of culture media is critical for commercial-scale production of recombinant proteins in E. coli. Addition of yeast extract and glucose to medium enhances rhIL24 production, and the use of lactose instead of IPTG for induction drops the cost and decreases toxicity. In addition, a two-step denaturing and one-step refolding (2DR) strategy improves rhIL24 production. The 2DR strategy replaces a more conventional approach for protein solubilization and refolding. LC–MS/MS provides definitive identification and quantitative information on rhIL24. Single-step purified rhIL24 displayed biological activity on HepG2 hepatocellular carcinoma cells, but no effect on L02 cells. Proliferation analysis suggests that rhIL24 may have potential use as a medication. In the present study, we developed a simple process for producing quality product with high purity. The expression and purification of rhIL24 described here may be a step towards inexpensive large-scale production.
Bioinformatics tools for genome mining of polyketide and non-ribosomal peptides
Authors: Boddy Christopher N 
Published: October 2013

Abstract
Microbial natural products have played a key role in the development of clinical agents in nearly all therapeutic areas. Recent advances in genome sequencing have revealed that there is an incredible wealth of new polyketide and non-ribosomal peptide natural product diversity to be mined from genetic data. The diversity and complexity of polyketide and non-ribosomal peptide biosynthesis has required the development of unique bioinformatics tools to identify, annotate, and predict the structures of these natural products from their biosynthetic gene clusters. This review highlights and evaluates web-based bioinformatics tools currently available to the natural product community for genome mining to discover new polyketides and non-ribosomal peptides.
SlnM gene overexpression with different promoters on natamycin production in Streptomyces lydicus A02
Authors: Wu Huiling , Liu Weicheng , Dong Dan , Li Jinjin , Zhang Dianpeng , Lu Caige 
Published: October 2013

Abstract
Natamycin is an important polyene macrolide antifungal agent produced by several Streptomyces strains and is widely used as a food preservative and fungicide in food, medicinal and veterinary products. In order to increase the yield of natamycin, this study aimed at cloning and overexpressing a natamycin-positive regulator, slnM2, with different promoters in the newly isolated strain Streptomyces lydicus A02, which is capable of producing natamycin. The slnM gene in S. lydicus is highly similar to gene pimM (scnRII), the pathway-specific positive regulator of natamycin biosynthesis in S. natalensis and S. chattanoogensis, which are PAS-LuxR regulators. Three engineered strains of S. lydicus, AM01, AM02 and AM03, were generated by inserting an additional copy of slnM2 with an ermEp* promoter, inserting an additional copy of slnM2 with dual promoters, ermEp* and its own promoter, and inserting an additional copy of slnM2 with its own promoter, respectively. No obvious changes in growth were observed between the engineered and wild-type strains. However, natamycin production in the engineered strains was significantly enhanced, by 2.4-fold in strain AM01, 3.0-fold in strain AM02 and 1.9-fold in strain AM03 when compared to the strain A02 in YEME medium without sucrose. These results indicated that the ermEp* promoter was more active than the native promoter of slnM2. Overall, dual promoters displayed the highest transcription of biosynthetic genes and yield of natamycin.
Enhancement of ansamitocin P-3 production in Actinosynnema pretiosum by a synergistic effect of glycerol and glucose
Authors: Gao Yang , Fan Yuxiang , Nambou Komi , Wei Liujing , Liu Zhijie , Imanaka Tadayuki , Hua Qiang 
Published: October 2013

Abstract
Ansamitocin P-3 (AP-3), a secondary metabolite produced by Actinosynnema pretiosum, is well known for its extraordinary antitumor properties and is broadly utilized in clinical research. Through this work, we found, for the first time, that the combination of glucose and glycerol as a mixed carbon source is an appropriate approach for enhancing the production of AP-3 by A. pretiosum. The amount yielded was about threefold that obtained with glucose as the sole carbon source. In order to better understand the mechanisms that channel glycerol metabolism towards AP-3 production, the activities of some key enzymes such as glucose-6-phosphate dehydrogenase, glucose-6-phosphate isomerase, phosphoglucomutase (PGM), and fructose 1,6-bisphosphatase were assessed. The results showed that glycerol affects the production of AP-3 by increasing PGM activity. Furthermore, qRT-PCR analysis revealed that transcriptional levels of structural genes asm14 and asm24, and primary genes amir5189 and amir6327 were up-regulated in medium containing glycerol.
Prevalence of Fabry disease in male patients with unexplained left ventricular hypertrophy in primary cardiology practice: prospective Fabry cardiomyopathy screening study (FACSS)
Authors: Palecek Tomas , Honzikova Jitka , Poupetova Helena , Vlaskova Hana , Kuchynka Petr , Golan Lubor , Magage Sudheera , Linhart Ales 
Published: October 2013

AbstractBackgroundA number of studies have already investigated the prevalence of Fabry disease (FD) in adult patients with unexplained left ventricular hypertrophy (LVH) with rates varying from 0 % up to 12 % reflecting referral and gender bias as well as differences in diagnostic methodology. We aimed to perform a prospective screening study evaluating the prevalence of FD in male patients older than 30 years with strictly defined unexplained LVH followed by general cardiologists.MethodsA predefined number of 100 men with unexplained LVH, defined as maximal wall thickness ≥ 13 mm, were identified during an echocardiographic examination in primary cardiology practice and screened by assessing α‐galactosidase A activity in dried blood spots (DBS) or in plasma.ResultsFour men (52 ± 4 years, maximal LV wall thickness 18 ± 3 mm) were diagnosed with FD confirmed by enzyme analysis in leukocytes as well as by genetic analysis. Mild extracardiac manifestations of FD were present in two of them.ConclusionsThe prevalence of FD in our cohort of male patients followed in primary cardiology practice with strictly defined otherwise unexplained LVH was 4 %. We recommend systematic screening for FD in all men older than 30 years with LVH of unknown etiology even in the absence of obvious extracardiac manifestations of FD.
Comparing reports of peer rejection: Associations with rejection sensitivity, victimization, aggression, and friendship
Authors: Zimmer‐Gembeck Melanie J. , Nesdale Drew , McGregor Leanne , Mastro Shawna , Goodwin Belinda , Downey Geraldine 
Published: October 2013

AbstractPerceiving that one is rejected is an important correlate of emotional maladjustment. Yet, self‐perceptions can substantially differ from classmate‐reports of who is rejected. In this study, discrepancies between self‐ and classmate‐reports of rejection were identified in 359 Australian adolescents (age 10–12 years). As expected, adolescents who overestimated rejection reported more rejection sensitivity and felt more victimized by their peers, but were not seen by peers as more victimized. Adolescents who underestimated rejection identified themselves as high in overt aggression, and their peers identified them as high in overt and relational aggression and low in prosocial behavior. Yet, underestimators' feelings of friendship satisfaction did not seem to suffer and they reported low rejection sensitivity. Results suggest that interventions to promote adolescent health should explicitly recognize the different needs of those who do and do not seem to perceive their high rejection, as well as adolescents who overestimate their rejection.
Remarks on Murre's conjecture on Chow groups
Authors: Xu Kejian , Xu Ze 
Published: October 2013

AbstractFor certain product varieties, Murre's conjecture on Chow groups is investigated. More precisely, let k be an algebraically closed field, X be a smooth projective variety over k and C be a smooth projective irreducible curve over k with function field K. Then we prove that if X (resp. XK) satisfies Murre's conjectures (A) and (B) for a set of Chow-Künneth projectors {, 0 ≤ i ≤ 2dim X} of X (resp. for {()K} of XK) and if for any j, , then the product variety X × C also satisfies Murre's conjectures (A) and (B). As consequences, it is proved that if C is a curve and X is an elliptic modular threefold over k (an algebraically closed field of characteristic 0) or an abelian variety of dimension 3, then Murre's conjecture (B) is true for the fourfold X × C.
DOCUMENTS, ARCHIVES, AND PROOF AROUND 1700
Authors: HEAD RANDOLPH C. 
Published: October 2013

ABSTRACTJean Mabillon'sDe re diplomatica, whose importance for diplomatics and the philosophy of history is well recognized, also contributed to the seventeenth-century European debate over the relationship among documents, archives, and historical or juridical proof. This article juxtaposes early works on diplomatics by Mabillon, Daniel Papebroche, and Barthélémy Germon against Germanius archivitheorists including Rutger Ruland and Ahasver Fritsch to reveal two incommensurate approaches that emerged around 1700 for assessing the authority of written records. Diplomatics concentrated on comparing the material and textual features of individual documents to authentic specimens in order to separate the genuine from the spurious, whereas theius archiviemphasized thepublica fides(public faith) that documents derived from their placement in an authentic sovereign's archive. Diplomatics' emergence as a separate auxiliary science of history encouraged the erasure of archivality from the primary conditions of documentary assessment for historians, however, while theius archivi's privileging of institutional over material criteria for authority foreshadowed European state practice and the evolution of archivistics into the twentieth century. This article investigates these competing discourses of evidence and their implications from the perspective of early modern archival practices.
FREDERICK, PRINCE OF WALES, AND THE FIRST PERFORMANCE OF ‘RULE, BRITANNIA!’
Authors: COX OLIVER J. W. 
Published: October 2013

ABSTRACTThe words and music of ‘Rule, Britannia!’ are synonymous with the expansionist, triumphalist, and imperialist Britain symbolized by fluttering Union Jacks on the Last Night of the Proms. This article explores the cultural and political contexts of the first performance of this important national cultural artefact as the finale ofAlfred: a masqueto suggest that this opening night served a very different purpose. The first audience was a court in exile from the metropolitan heart of London, popular amongst the general public, but without any prospects of government. Two of the most important members of this group of peers, politicians, poets and a prince had recently died, and with them any cohesive identity.Alfredis both a desperate plea for unity, a rallying cry which forcefully restated the key tenets of this group's identity, and a delayed expression of patriotic celebration occasioned by Admiral Vernon's capture of Portobello. Through addressing this performance, this article makes an important contribution to our understanding of Hanoverian political culture and highlights the continuing impact of Anglo-Saxon England on mid-eighteenth-century Britain.
The Impact of Government Intervention in Banks on Corporate Borrowers’ Stock Returns
Authors: Norden Lars , Roosenboom Peter , Wang Teng 
Published: October 2013

AbstractWe investigate whether and how government interventions in the U.S. banking sector influence the stock market performance of corporate borrowers during the financial crisis of 2007–2009. We measure firms’ exposures to government interventions with an intervention score that is based on combined information on the firms’ structure of bank relationships and their banks’ participation in government capital support programs. We find that government capital infusions in banks have a significantly positive impact on borrowing firms’ stock returns. The effect is more pronounced for riskier and bank-dependent firms and for those that borrow from banks that are less capitalized and smaller.