bluuebunny/crossref_metadata_2025_split huggingface dataset:
Screening of botanical spatial mosquito repellents from 64 essential oils and their interactions with odorant‐binding protein‐3
Authors: Ren Xiaofei , Li Wenjiao , Zhang Jinmiao , Zhu Yingxiang , Hou Xiaohui , Miao Hanwen , Xu Ximing , Zhang Dongjing , Sheng Zhaojun 
Published: July 2024

AbstractPersonal protection from mosquito bites is essential for preventing mosquito‐borne diseases. Mosquito repellents are effective methods for preventing mosquito bites. Due to their ease of use and indirect skin contact, mosquito‐repellent stickers loaded with essential oils (EOs) have recently become favoured as novel spatial repellents. To discover active EOs with high spatial repellency, 64 commercial EOs were screened using a modified arm‐in‐cage assay. Four EOs, including nutmeg, melissa, eucalyptus and cinnamon, displayed longer complete protection times (CPTs) of about 60 min. Ten main constituents from the four active EOs were identified by gas chromatography–mass spectrometry (GC–MS), and their spatial repellent activities were further evaluated. 1,8‐Cineole, cinnamaldehyde and citral showed potent repellent activity, with average CPTs of 67.5, 63.8 and 60 min respectively. The interactions of 1,8‐cineole and citral with odorant‐binding protein‐3 (OBP‐3) were studied via molecular docking. Both compounds are docked well in the high hydrophobic pocket and interacted with AealOBP3 through Van der Waals interactions with Phe105, Phe112, Leu114, Met48 and Val50. These natural products provide promising lead fragments for the further development of botanical spatial repellents.
Morphology, behavior, and phylogenomics of Oxytoxum lohmannii, Dinoflagellata
Authors: Cooney Elizabeth C. , Jacobson Dean M. , Wolfe Gordon V. , Bright Kelley J. , Saldarriaga Juan F. , Keeling Patrick J. , Leander Brian S. , Strom Suzanne L. 
Published: July 2024

AbstractDinoflagellates are an abundant and diverse group of protists representing a wealth of unique biology and ecology. While many dinoflagellates are photosynthetic or mixotrophic, many taxa are heterotrophs, often with complex feeding strategies. Compared to their photosynthetic counterparts, heterotrophic dinoflagellates remain understudied, as they are difficult to culture. One exception, a long‐cultured isolate originally classified as Amphidinium but recently reclassified as Oxytoxum, has been the subject of a number of feeding, growth, and chemosensory studies. This lineage was recently determined to be closely related to Prorocentrum using phylogenetics of ribosomal RNA gene sequences, but the exact nature of this relationship remains unresolved. Using transcriptomes sequenced from culture and three single cells from the environment, we produce a robust phylogeny of 242 genes, revealing Oxytoxum is likely sister to the Prorocentrum clade, rather than nested within it. Molecular investigations uncover evidence of a reduced, nonphotosynthetic plastid and proteorhodopsin, a photoactive proton pump acquired horizontally from bacteria. We describe the ultrastructure of O. lohmannii, including densely packed trichocysts, and a new type of mucocyst. We observe that O. lohmannii feeds preferentially on cryptophytes using myzocytosis, but can also feed on various phytoflagellates using conventional phagocytosis. O. lohmannii is amenable to culture, providing an opportunity to better study heterotrophic dinoflagellate biology and feeding ecology.
Naming the other cousin: A new goldie barb (Cyprinidae: Smiliogastrininae) from the northeast escarpment in South Africa, with proposed taxonomic rearrangement of the goldie barb group in southern Africa
Authors: Scheepers Martinus , Bragança Pedro H. N. , Chakona Albert 
Published: July 2024

AbstractA growing body of evidence indicates that the global diversity of freshwater fishes has not been fully documented. Studies of freshwater fishes that were previously thought to be morphologically variable have revealed the existence of deeply divergent lineages, with many distinct species. In southern Africa a number of Enteromius species exhibit either exceedingly wide or divided distribution patterns that should be rare for freshwater fishes with limited dispersal opportunities between river systems. One such species is the sidespot barb, Enteromius neefi. As currently defined, E. neefi has a disjunct distribution that is divided between rivers in the northeast escarpment in South Africa and Eswatini, and tributaries of the Upper Zambezi in Zambia and southern Congo in the Democratic Republic of Congo, with a large geographic gap between these two populations. With the use of molecular and morphological methods, the level of divergence between the two populations was examined, and a new species was described from the Steelpoort River in the Limpopo River system of South Africa. Findings from this study provide further evidence for a number of taxonomic problems within the goldie barbs of southern Africa, and some taxonomic rearrangements are proposed for this group.
Barriers and facilitators to evidence‐based perioperative hypothermia management for orthopaedic patients: A systematic review
Authors: Heidke Penny , Duff Jed , Keogh Samantha , Munday Judy 
Published: July 2024

AbstractAimTo assess barriers and facilitators to the implementation of guidelines for the prevention of inadvertent perioperative hypothermia in orthopaedic patients.DesignSystematic review.Data SourcesNine databases: PubMed, Embase, CINAHL, Cochrane CENTRAL, PsycINFO, ProQuest Dissertations and Theses, Scopus, Web of Science and Trip Clinical Evidence Database.MethodsPrimary studies published in English between January 2008 to July 2022 were screened. Study selection, quality assessment, and data extraction were completed independently by researchers. Data were extracted using the Consolidated Framework for Implementation Research and mapped to the Expert Recommendations for Implementing Change strategies.ResultsEighty‐seven studies were included in the review. The most frequently reported barriers and facilitators related to evidence strength, relative advantage, and cost of implementing perioperative hypothermia prevention guidelines. The top four ERIC strategies were: Identify and prepare champions; Conduct educational meetings; Assess for readiness and identify barriers and facilitators; and Inform local opinion leaders.ConclusionThis review provides synthesized evidence regarding barriers and facilitators to perioperative hypothermia guidelines for patients undergoing orthopaedic surgery.Implications for the Profession and Patient CareOur work provides theory guided strategies to promote implementation of perioperative hypothermia prevention to assist nurses caring for patients undergoing orthopaedic surgery.ImpactFindings provide professionals caring for patients undergoing orthopaedic surgery with theory‐informed strategies to improve perioperative hypothermia prevention. Reducing perioperative hypothermia will improve outcomes for patients undergoing orthopaedic surgery.Reporting MethodThe review is reported according to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses 2020.No Patient or Public ConsultationDue to the study design, no patient or public consultation took place.
Very Noisy Option Prices and Inference Regarding the Volatility Risk Premium
Authors: DUARTE JEFFERSON , JONES CHRISTOPHER S. , WANG JUNBO L. 
Published: July 2024

ABSTRACTThe stylized fact that volatility is not priced in individual equity options does not withstand scrutiny. First, we show that the average return of heavily traded deep out‐of‐the‐money call options on stocks is −116 basis points per day. Second, Fama‐MacBeth estimates of the volatility risk premium in stock options are similar to those in S&P 500 Index call options. Third, the mean return of heavily traded delta‐hedged at‐the‐money calls (puts) is −23 (−30) basis points. Fourth, the variance risk premium in stock options is negative. Our analysis highlights the importance of microstructure biases and robustness in empirical work with options.
Forest genomics in the Caucasus through the lens of its dominant tree species – Fagus orientalis
Authors: Capblancq Thibaut , Sękiewicz Katarzyna , Dering Monika 
Published: July 2024

AbstractThe last glacial period is known to have greatly influenced the demographic history of temperate forest trees, with important range contractions and post‐glacial expansions that led to the formation of multiple genetic lineages and secondary contact zones in the Northern Hemisphere. These dynamics have been extensively studied for European and North American species but are still poorly understood in other temperate regions of rich biodiversity such as the Caucasus. Our study helps filling that gap by deciphering the genomic landscapes of F. orientalis across the South Caucasus. The use of genome‐wide data confirmed a past demographic history strongly influenced by the Last Glacial Maximum, revealing two disjunct glacial refugia in the Colchis and Hyrcanian regions. The resulting patterns of genetic diversity, load and differentiation are not always concordant across the region, with genetic load pinpointing the location of the glacial refugia more efficiently than genetic diversity alone. The Hyrcanian forests show depleted genetic diversity and substantial isolation, even if long‐distance gene flow is still present with the main centre of diversity in the Greater Caucasus. Finally, we characterize a strong heterogeneity of genetic diversity and differentiation along the species chromosomes, with noticeably a first chromosome showing low diversity and weak differentiation.
Ion Channels Remodeling in the Regulation of Vascular Hyporesponsiveness During Shock
Authors: Li Keqing , Li Yuan , Chen Yinghong , Chen Tangting , Yang Yan , Li Pengyun 
Published: July 2024

ABSTRACTShock is characterized with vascular hyporesponsiveness to vasoconstrictors, thereby to cause refractory hypotension, insufficient tissue perfusion, and multiple organ dysfunction. The vascular hyporeactivity persisted even though norepinephrine and fluid resuscitation were administrated, it is of critical importance to find new potential target. Ion channels are crucial in the regulation of cell membrane potential and affect vasoconstriction and vasodilation. It has been demonstrated that many types of ion channels including K+ channels, Ca2+ permeable channels, and Na+ channels exist in vascular smooth muscle cells and endothelial cells, contributing to the regulation of vascular homeostasis and vasomotor function. An increasing number of studies suggested that the structural and functional alterations of ion channels located in arteries contribute to vascular hyporesponsiveness during shock, but the underlying mechanisms remained to be fully clarified. Therefore, the expression and functional changes in ion channels in arteries associated with shock are reviewed, to pave the way for further exploring the potential of ion channel‐targeted compounds in treating refractory hypotension in shock.
Piloting and watch over in the end‐of‐life care of intensive care unit patients with COVID‐19—A qualitative study
Authors: Pettersson Charlotte , Forsén Johanna , Joelsson‐Alm Eva , Fridh Isabell , Björling Gunilla , Mattsson Janet 
Published: July 2024

AbstractBackgroundDuring the COVID‐19 pandemic, intensive care units (ICUs) were under heavy pressure, with a significantly increased number of severely ill patients. Hospitals introduced restrictions, and families could not visit their ill and dying family members. Patients were cared for without privacy, and several died in shared patient rooms, leaving the intensive care nurse to protect the patient's need for loving care in a vulnerable situation at the end of life.AimThis study aimed to investigate how piloting and watch over were revealed in end‐of‐life care for patients with COVID‐19 in intensive care COVID‐19.Study DesignA qualitative study was conducted with an abductive approach was conducted. Data were collected via semi‐structured interviews to cover the research area while allowing the informant to talk freely about the topic; 11 informants were interviewed.ResultsThe findings are presented based on four categories: The road to the decision, End‐of‐life care, Farewell of close family members and Closure. Each category and subcategory reveal how piloting and watch over were addressed in the end‐of‐life care of patients with COVID‐19 in the ICU during the pandemic. Overall findings indicated that workload and organization of care directly affect the quality of care given, the acceptance of privacy and the possibility of dignified end‐of‐life care.ConclusionsWorkload directly affects the quality of care, risking dehumanization of the patient. Visiting restrictions hindered supporting family members through the various piloting phases. Visiting restrictions also forced the ICU nurses to take on the role of the relative in watching over the patient.Relevance to Clinical PracticeCollaboration with family members is essential for the intensive care nurse to be able to provide a person‐centred and dignified end‐of‐life care.
Efficacy of sugar‐protein non‐membranous dietary system for diapause egg production in Aedes albopictus mosquitoes under short‐day conditions
Authors: Suman Devi Shankar , Chandel Kshitij , Wang Yi , Gaugler Randy 
Published: July 2024

AbstractThe Asian tiger mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae), is a global vector of dengue, chikungunya and zika viruses. With extreme adaptability of survival as diapause eggs, this mosquito has invaded and established in temperate climatic zones. The diapause eggs are specially programmed to overcome the harsh winter conditions in temperate habitats. In the laboratory, diapausing females are reared from early larval instars under short‐day conditions (21°C and 16D:8 L photoperiods). Aedes albopictus is normally fed on Guinea pigs for the rearing of diapause mosquitoes which requires ethical approval from the animal use committee and costly arrangements. Therefore, the present study was conducted to find whether bovine serum albumin protein (10%) with sucrose (5%) solution (SAP‐10) offered through a membrane‐free feeding system would result in an alternative diet for the production of diapause eggs in comparison to blood‐feeding. This feeding system shows a considerable rate of engorgement of diapausing females under short‐day conditions and produced 30.8 diapause eggs/female in comparison to blood‐feeding on a guinea pig (40.9 eggs/female). The diapause rate of viable eggs for the SAP‐10 diet (95.28%) was similar to the blood‐feeding (96.32%). The findings suggest that the present diet feeding system is a promising tool for the rearing of diapause Ae. albopictus mosquitoes under short‐day conditions. Further studies are suggested to evaluate the physiological and developmental aspects of mosquitoes reared on the diet.
COPD is associated with increased cardiovascular disease risk independent of phenotype
Authors: Cobb Kolton , Kenyon Jonathan , Lu Juan , Krieger Benjamin , Perelas Apostolos , Nana‐Sinkam Patrick , Kim Youngdeok , Rodriguez‐Miguelez Paula 
Published: July 2024

AbstractBackground and ObjectiveChronic obstructive pulmonary disease (COPD) is a leading cause of death worldwide that frequently presents with concomitant cardiovascular diseases. Despite the pathological distinction between individual COPD phenotypes such as emphysema and chronic bronchitis, there is a lack of knowledge about the impact of COPD phenotype on cardiovascular disease risk. Thus, this study aimed to utilize a nationally representative sample to investigate cardiovascular disease prevalence in patients with COPD with emphysema and chronic bronchitis phenotypes.MethodsData from 31,560 adults including 2504 individuals with COPD, collected as part of the National Health and Nutrition Examination Survey (1999–2018), were examined.ResultsA significantly increased cardiovascular disease risk, including coronary heart disease, heart failure, myocardial infarction and stroke, was identified in patients with COPD among all disease phenotypes. Particularly, compared to those without COPD, individuals with chronic bronchitis presented with 1.76 (95% CI: 1.41–2.20) times greater odds, individuals with emphysema with 2.31 (95% CI: 1.80–2.96) times greater odds, while those with a concurrent phenotype (combined chronic bronchitis and emphysema) exhibited 2.98 (95% CI: 2.11–4.21) times greater odds of reporting cardiovascular diseases.ConclusionOur data confirms that patients with COPD present an elevated risk of developing cardiovascular disease among all phenotypes, with the most marked increase being in those with concurrent chronic bronchitis and emphysema phenotypes. These findings emphasize the need for awareness and appropriate cardiovascular screening in COPD.image
The shadow wage of child labor: An application to Nepal
Authors: Meneghello Elisa , Menon Martina , Perali Federico , Rosati Furio 
Published: July 2024

AbstractThis paper describes a new method for estimating shadow wages and identifying the shadow contribution of child labor to household income. The approach enables a direct test for recursivity by comparing the estimated shadow wages with the market wage. This is a novel option to test for non‐separability that complements traditional indirect tests based on constraining production or consumption decisions. Our innovative identification strategy is not specific to child labor but can also be used to identify gender‐specific shadow wages for women and men. The estimated shadow wages are meaningful in the context of Nepal's rural economy. Based on the results of our direct test for separability, we conclude that the separable representation of farm households is inconsistent with the Nepalese data. We also estimate the contribution of child labor to household income at both the household and national levels. A series of simulations illustrates the role that child labor plays in household livelihoods and how it affects Nepal's income distribution.
Tuberculosis and labour market participation: Evidence from South Africa
Authors: Ting Helena , Bozzola Martina , Ravetti Chiara 
Published: July 2024

AbstractTuberculosis (TB) is one of the leading causes of death from an infectious disease, but its micro‐economic impacts on labour market participation remain poorly understood. We analyse the relationship between TB and employment outcomes in South Africa, one of the countries with the highest TB disease burden worldwide, using individual‐level panel data from 2008 to 2017. Applying a coarsened exact matching methodology, we find that contracting TB entails a 5% lower probability of entering the formal labour market. Moreover, TB and its associated employment changes go hand in hand with corresponding reductions in individual income but not in household income and expenditure.
Hourly to weekly variations in halite precipitation from the hypersaline Dead Sea: The role of evaporation, water cooling and freshwater plume stability
Authors: Mor Ziv , Noy Gil , Eyal Haggai , Sirota Ido , Ezraty Roie , Morin Efrat , Lensky Nadav G. 
Published: July 2024

ABSTRACTThick‐bedded halite sequences in the geological record are characterized by lateral variations, reflecting spatiotemporal variations in environmental forcing. Although temporal variations have been examined over seasonal scales or longer durations, there is a paucity of information regarding the dynamics of halite deposition at shorter durations of days and hours. Additionally, the influence of water discharges and surface salinity gradients remains poorly understood. Here, for the first time, in situ, hourly to weekly halite accumulation rates were measured on the Dead Sea floor (ca 20 m water depth), the only modern deep and hypersaline water body. Novel underwater photography methods were developed to directly observe halite precipitation at the lake floor, coeval with monitoring of the environmental drives, i.e. evaporation, meteorological and limnological conditions. Two fundamental environments were explored: a diluted plume environment arising from spring discharge; and a mixed hypersaline environment. The focus was on deposition during winter when the halite accumulation rate is highest, and the relationship between evaporation and halite accumulation is most straightforward since the water column is vertically uniform. Significant spatial variations in halite accumulation were found between the two environments, along with hourly variations in each environment. In the mixed hypersaline environment, over intra‐diurnal timescales, evaporation plays a more significant role in halite precipitation, whereas temperature has a greater impact on seasonal changes. In the diluted plume environment, the stability of the buoyant plume controls the rate of halite deposition; during windstorms, the plume is stirred, with higher surface water salinity and higher rate of halite accumulation. However, under calm winds, the plume is stable, with diluted surface water, and the rate of halite accumulation is a few times lower. Following these new findings, the implications for the interpretation of halite deposits are discussed.
The auxin‐responsive CsSPL9‐CsGH3.4 module finely regulates auxin levels to suppress the development of adventitious roots in tea (Camellia sinensis)
Authors: Wang Wenzhao , Jiao Mengmin , Huang Xue , Liang Wenjuan , Ma Zhonglian , Lu Zhanling , Tian Shenyang , Gao Xiuhua , Fan Li , He Xinyue , Bao Junhua , Yu Youben , Zhang Dong , Bao Lu 
Published: July 2024

SUMMARYThe cutting technique is extensively used in tea breeding, with key emphasis on promoting the growth of adventitious roots (ARs). Despite its importance in tea cultivation, the mechanisms underlying AR development in tea remain unclear. In this study, we demonstrated the essential role of auxins in the initiation and progression of AR and established that the application of exogenous 1‐naphthaleneacetic acid‐enhanced AR formation in tissue‐cultured seedlings and cuttings. Then, we found that the auxin‐responsive transcription factor CsSPL9 acted as a negative regulator of AR development by reducing the levels of free indole‐3‐acetic acid (IAA) in tea plants. Furthermore, we identified CsGH3.4 as a downstream target of CsSPL9, which was activated by direct binding to its promoter. CsGH3.4 also inhibited AR development and maintained low levels of free IAA. Thus, these results revealed the inhibitory effect of the auxin‐responsive CsSPL9‐CsGH3.4 module on AR development by reducing free IAA levels in tea. These findings have significant theoretical and practical value for enhancing tea breeding practices.