bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenic Mechanisms in Lupus Nephritis: Nucleosomes Bind Aberrant Laminin β1 With High Affinity and Colocalize in the Electron‐Dense Deposits
Authors: Olin Anders I. , Mörgelin Matthias , Truedsson Lennart , Sturfelt Gunnar , Bengtsson Anders A. 
Published: October 2013

ObjectiveApoptotic nucleosomes are structurally and immunologically involved in lupus nephritis. The purpose of this study was to examine the expression and function of laminins and their interactions with nucleosomes in the kidneys of patients with lupus nephritis, using surface plasmon resonance (SPR) analysis.MethodsSPR interaction analysis was used to quantify the strength of laminin–nucleosome interactions. Electron microscopy techniques were used to determine in vivo colocalization of IgG, chromatin, and laminin β1, as well as to characterize nucleosome–laminin interactions in vitro.ResultsNucleosomes were found to possess high affinity for laminin β1–containing laminins and to have the potential to form stable molecular complexes with these structures. In vivo, laminin β1 was aberrantly expressed in the glomerular basement membrane (GMB) of lupus nephritis patients, and in situ, it acted as a nucleosome ligand, selectively colocalizing with nucleosomes within electron‐dense deposits (EDDs). Normal adult laminin 11, which contains laminin β2, did not bind nucleosomes, and it did not colocalize in vivo with the nucleosomes in the nephritic GBM. In addition, TGFβ1 was expressed by the glomerular mesangium, glomerular endothelial cells, and by podocytes in patients with lupus nephritis. It was trapped in situ within EDDs by an as‐yet‐unknown ligand. As was recently described in a transgenic mouse model, paracrine kidney glomerular TGFβ1 may thereby contribute to the development of glomerulopathy via the induction of laminin β1 incorporation in the GBM, whereas systemic blood vessel–derived TGFβ1 could be trapped during filtration.ConclusionOur findings of the specific high‐affinity binding of nucleosomes to aberrantly expressed laminin β1 in the GBM and their colocalization in the GBM may explain important features of the initial steps in the pathogenesis of lupus nephritis, the planted antigen hypothesis.
Improving the representation of peptide‐like inhibitor and antibiotic molecules in the Protein Data Bank
Authors: Dutta Shuchismita , Dimitropoulos Dimitris , Feng Zukang , Persikova Irina , Sen Sanchayita , Shao Chenghua , Westbrook John , Young Jasmine , Zhuravleva Marina A. , Kleywegt Gerard J. , Berman Helen M. 
Published: October 2013

ABSTRACTWith the accumulation of a large number and variety of molecules in the Protein Data Bank (PDB) comes the need on occasion to review and improve their representation. The Worldwide PDB (wwPDB) partners have periodically updated various aspects of structural data representation to improve the integrity and consistency of the archive. The remediation effort described here was focused on improving the representation of peptide‐like inhibitor and antibiotic molecules so that they can be easily identified and analyzed. Peptide‐like inhibitors or antibiotics were identified in over 1000 PDB entries, systematically reviewed and represented either as peptides with polymer sequence or as single components. For the majority of the single‐component molecules, their peptide‐like composition was captured in a new representation, called the subcomponent sequence. A novel concept called “group” was developed for representing complex peptide‐like antibiotics and inhibitors that are composed of multiple polymer and nonpolymer components. In addition, a reference dictionary was developed with detailed information about these peptide‐like molecules to aid in their annotation, identification and analysis. Based on the experience gained in this remediation, guidelines, procedures, and tools were developed to annotate new depositions containing peptide‐like inhibitors and antibiotics accurately and consistently. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 659–668, 2014.
Controlling network topology and mechanical properties of co‐assembling peptide hydrogels
Authors: Boothroyd Stephen , Saiani Alberto , Miller Aline F. 
Published: October 2013

ABSTRACTOligopeptides are well‐known to self‐assemble into a wide array of nanostructures including β‐sheet‐rich fibers that when present above a critical concentration become entangled and form self‐supporting hydrogels. The length, quantity, and interactions between fibers influence the mechanical properties of the hydrogel formed and this is typically achieved by varying the peptide concentration, pH, ionic strength, or the addition of a second species or chemical cross‐linking agent. Here, we outline an alternative, facile route to control the mechanical properties of the self‐assembling octa‐peptide, FEFEFKFK (FEKII); simply doping with controlled quantities of its double length peptide, FEFEFKFK‐GG‐FKFKFEFE (FEKII18). The structure and properties of a series of samples were studied here (0–100 M% of FEKII18) using Fourier transform infrared, small angle X‐ray scattering, transmission electron microscopy, and oscillatory rheology. All samples were found to contain elongated, flexible fibers and all mixed samples contained Y‐shaped branch points and parallel fibers which is attributed to the longer peptide self‐assembling within two fibers, thus creating a cross‐link in the network structure. Such behavior was reflected in an increase in the elasticity of the mixed samples with increasing quantity of double peptide. Interestingly the elastic modulus increased up to 30 times the pure FEKII value simply by adding 28 M% of FEKII18. These observations provide an easy, off‐the‐shelf method for an end‐user to control the cross‐linked network structure of the peptide hydrogel, and consequently strength of the hydrogel simply by physically mixing pre‐determined quantities of two similar peptide molecules. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 669–680, 2014.
Expression profiling of cervical cancers in Indian women at different stages to identify gene signatures during progression of the disease
Authors: Thomas Asha , Mahantshetty Umesh , Kannan Sadhana , Deodhar Kedar , Shrivastava Shyam K. , Kumar‐Sinha Chandan , Mulherkar Rita 
Published: October 2013

AbstractCervical cancer is the second most common cancer among women worldwide, with developing countries accounting for >80% of the disease burden. Although in the West, active screening has been instrumental in reducing the incidence of cervical cancer, disease management is hampered due to lack of biomarkers for disease progression and defined therapeutic targets. Here we carried out gene expression profiling of 29 cervical cancer tissues from Indian women, spanning International Federation of Gynaecology and Obstetrics (FIGO) stages of the disease from early lesion (IA and IIA) to progressive stages (IIB and IIIA–B), and identified distinct gene expression signatures. Overall, metabolic pathways, pathways in cancer and signaling pathways were found to be significantly upregulated, while focal adhesion, cytokine–cytokine receptor interaction and WNT signaling were downregulated. Additionally, we identified candidate biomarkers of disease progression such as SPP1, proliferating cell nuclear antigen (PCNA), STK17A, and DUSP1 among others that were validated by quantitative real‐time polymerase chain reaction (qRT‐PCR) in the samples used for microarray studies as well in an independent set of 34 additional samples. Integrative analysis of our results with other cervical cancer profiling studies could facilitate the development of multiplex diagnostic markers of cervical cancer progression.
HighRAB25expression is associated with good clinical outcome in patients with locally advanced head and neck squamous cell carcinoma
Authors: Téllez‐Gabriel Marta , Arroyo‐Solera Irene , León Xavier , Gallardo Alberto , López Montserrat , Céspedes Maria V. , Casanova Isolda , López‐Pousa Antonio , Quer Miquel , Mangues Maria A. , Barnadas Agustí , Mangues Ramón , Pavón Miguel A. 
Published: October 2013

AbstractCurrently there are no molecular markers able to predict clinical outcome in locally advanced head and neck squamous cell carcinoma (HNSCC). In a previous microarray study,RAB25was identified as a potential prognostic marker. The aim of this study was to analyze the association betweenRAB25expression and clinical outcome in patients with locally advancedHNSCCtreated with standard therapy. In a retrospective immunohistochemical study (n = 97), we observed thatRAB25‐negative tumors had lower survival (log‐rank,P = 0.01) than patients bearing positive tumors. In an independent prospectivemRNAstudy (n = 117), lowRAB25mRNAexpression was associated with poor prognosis. Using classification and regression tree analysis (CART) we established two groups of patients according to theirRAB25mRNAlevel and their risk of death. LowmRNAlevel was associated with poor local recurrence‐free (log‐rank,P = 0.005), progression‐free (log‐rank,P = 0.002) and cancer‐specific (log‐rank,P < 0.001) survival. Multivariate Cox model analysis showed that low expression ofRAB25was an independent poor prognostic factor for survival (hazard ratio: 3.84, 95% confidence interval: 1.93–7.62,P < 0.001). Patients whose tumors showed highRAB25expression had a low probability of death after treatment. We also found lowerRAB25expression in tumors than in normal tissue (Mann–WhitneyU,P < 0.001). Moreover, overexpression ofRAB25in theUM‐SCC‐74BHNSCCcell line increased cisplatin sensitivity, and reduced cell migration and invasion. Our findings support a tumor suppressor role forRAB25inHNSCCand its potential use to identify locally advanced patients with a high probability of survival after genotoxic treatment.
Cu2O‐Decorated Mesoporous TiO2 Beads as a Highly Efficient Photocatalyst for Hydrogen Production
Authors: Cheng Wei‐Yun , Yu Tsung‐Hsuan , Chao Kang‐Ju , Lu Shih‐Yuan 
Published: October 2013

AbstractCu2O‐decorated mesoporous TiO2 beads (MTBs) are developed as a low‐cost, highly efficient photocatalyst for H2 production. MTBs with a high specific surface area of 189 m2 g−1, a large pore volume of 0.43 cm3 g−1 and a suitable pore size of 8.9 nm are decorated with band‐structure‐matched Cu2O nanocrystals through a simple, fast and low‐cost chemical bath deposition process. The Cu2O nanocrystals serve as an electron–hole separation centre to promote H2 evolution. Under optimal operation conditions, an ultra‐high specific H2 evolution rate of 223 mmol h−1 g−1 is achieved. The success is attributed to the structural advantages of the MTBs of high specific surface areas, large pore volumes and suitable pore sizes together with the much improved electron–hole separation and light utilisation of the Cu2O‐decorated MTBs. The H2 evolution rates achieved with the Cu2O‐decorated MTBs are one order of magnitude higher than those achieved by commercial P25 TiO2.
Ionic Grubbs–Hoveyda Complexes for Biphasic Ring‐Opening Metathesis Polymerization in Ionic Liquids: Access to Low Metal Content Polymers
Authors: Ferraz Camila P. , Autenrieth Benjamin , Frey Wolfgang , Buchmeiser Michael R. 
Published: October 2013

AbstractA novel ionic metathesis catalyst, [Ru(1‐CH3‐4‐CO2Py+)2(IMesH2)(CH‐2‐{2‐PrO}‐5‐NO2C6H3)][OTf−]2 (3 b; IMesH2=1,3‐dimesitylimidazolin‐2‐ylidene, Py=pyridine), has been prepared. 3 b and the Grubbs–Hoveyda catalysts [Ru(1‐CH3‐4‐CO2Py+)2(IMesH2)(CH‐2‐{2‐PrO}C6H4)][OTf−]2 (3 a) and [RuCl(1‐CH3‐4‐CO2Py+)(IMesH2)(CH‐2‐{2‐PrO}C6H4)][OTf−] (5 a) were used for ring‐opening metathesis polymerization (ROMP) reactions under both homogeneous and biphasic liquid–liquid conditions by using the ionic liquid 1‐butyl‐2,3‐dimethylimidazolium tetrafluoroborate ([BDMIM+][BF4−]) and toluene. All catalysts were active in the ROMP of norborn‐2‐ene‐based monomers, with cis‐cyclooctene and dicyclopentadiene providing good yields under homogeneous conditions and complex 5 a the most active catalyst. With all catalysts, the use of a chain transfer agent (CTA) allowed for the synthesis of polymers with low metal contents between 10 and 80 ppm, corresponding to a ruthenium removal of 98–99.8 % without any additional purification step. In addition, the use of a CTA allowed for recycling experiments under biphasic conditions, in which 3 a and 5 a were particularly active for several cycles.
Convection Heat Transfer of CO2 at Supercritical Pressures in Microtubes
Authors: Yang F.‐Y. , Wang K. , Liu T. , Wang Y.‐Q. , Liu Z.‐C. 
Published: October 2013

AbstractConvection heat transfer of supercritical pressure CO2 in microtubes as well as characteristics of fluid flow and heat transfer were investigated. Alterations of physical properties of supercritical CO2 and the impact of buoyancy on the heat transfer were analyzed when the inlet Reynolds (Re) number of CO2, diameter of heat exchanger, and inlet Re of cooling water were changed. The temperatures of hot fluid in numerical simulations from several classical turbulence calculation models were compared with experimental results from the literature. The physical properties of CO2 changed significantly around the critical point, causing a distinct decline of buoyancy and strengthening of heat transfer.
Determination of Amino Acid Enantiopurity and Absolute Configuration: Synergism between Configurationally Labile Metal‐Based Receptors and Dynamic Covalent Interactions
Authors: Scaramuzzo Francesca A. , Licini Giulia , Zonta Cristiano 
Published: October 2013

AbstractReliable determination of the enantiomeric excess of free amino acids can be obtained by measuring the induced circular dichroism of a multicomponent assembly formed by a modified tris(2‐pyridylmethyl)amine ligand, a zinc salt, and the amino acid of interest. The systems furnish reliable information for all natural amino acids.
Numerical Simulation of Concentration‐Induced Marangoni Convection at Single Rising Droplets
Authors: Engberg Roland F. , Wegener Mirco , Kenig Eugeny Y. 
Published: October 2013

AbstractBeim Stofftransport an einem aufsteigenden Tropfen können Konzentrationsgradienten zu einer Variation der Grenzflächenspannung entlang der Phasengrenze führen und Marangoni‐Konvektion hervorrufen. Dadurch wird nicht nur der Stofftransport intensiviert, sondern auch das Aufstiegsverhalten des Tropfens beeinflusst. Um diese Phänomene mittels Strömungssimulationen zu untersuchen, wurde ein CFD‐Code entwickelt und in das CFD‐Paket OpenFOAM® implementiert, der der Kopplung von Impuls‐ und Stofftransport sowie der Verformung des Tropfens Rechnung trägt. Hier werden die Ergebnisse von 3D‐Simulationen vorgestellt und validiert.
Who benefits from market expansion?
Authors: Li Tieshan 
Published: October 2013

AbstractThis article presents a study of the effects of both symmetric and asymmetric market expansion on two retailers and their consumers. Envisioning two retailers along a Hotelling line, this article describes both the symmetric and asymmetric expansion of the market; the most interesting results occur when expansion is asymmetric. Applying game theory and showing the results numerically, I find three categories of unit‐distance moving costs incurred in three scenarios: when both retailers choose to move, when only one retailer chooses to move, and when neither retailer chooses to move. With fixed moving costs, moving is the dominant strategy for the retailer close to market expansion. Surprisingly, the retailer located the farthest away from the expansion has the highest profits. Copyright © 2013 ASAC. Published by John Wiley & Sons, Ltd.
Effects of morphine and aminophylline on the severity of obstruction to left ventricular outflow in idiopathic hypertrophic subaortic stenosis: Potential adverse effects in treatment of pulmonary edema
Authors: Awan N. A. , Miller R. R. , Demaria A. N. , Lee G. , Mason D. T. 
Published: October 2013

AbstractAlthough morphine sulfate and aminophylline are commonly employed in the management of acute pulmonary edema of diverse etiologies including idiopathic hypertrophic subaortic stenosis (IHSS), the effects of morphine and aminophylline on left ventricular (LV) outflow obstruction in IHSS are unknown. Thus to determine the actions of morphine and aminophylline in IHSS, seven patients with IHSS (mean peak resting systolic gradient 16 mmHg, mean Valsalva provoked gradient 87 mmHg) received 5 mg morphine intravenously and simultaneous LV and brachial arterial pressures were continously monitored and cardiac outputs obtained. On return to control hemodynamics, 250 mg aminophylline was infused in three patients and hemodynamics repeated. Peak increase in gradient with morphine occurred in 5 min in each patient and average maximal gradient was 48 mmHg (p<0.001). Peak LV systolic pressure increased 158 to 182 mmHg (p<0.05); LV end‐diastolic pressure declined 12 to 8 mmHg (p<0.005); cardiac index decreased 3.18 to 2.89 l/min/m2 (p<0.005); while heart rate and total systemic vascular resistance were unchanged (p>0.05). Aminophylline increased peak gradient to 57 mmHg; lowered LV end‐diastolic pressure by 2 mmHg; decreased total systemic vascular resistance 1610 to 1120 dyne s · cm−5; while cardiac index was unchanged. Therefore morphine and aminophylline can rapidly increase LV outflow obstruction to marked extents in IHSS. These data indicate morphine worsens subaortic stenosis by decrease in LV preload through systemic venodilation, whereas aminophylline increases intraventricular gradient predominantly by peripheral arterial dilation. Thus morphine and aminophylline should be avoided, or used cautiously in low doses, in management of acute pulmonary edema in IHSS.
The ischemic segment and left ventricular function during exercise angina
Authors: Rentrop P. , Karsch K. R. , Nitsche K. 
Published: October 2013

AbstractImprovement of the quality of transpulmonary left ventriculograms by exercise was demonstrated in 5 patients in a pre‐study. In the main study transpulmonary left ventriculography was performed in 10 patients with coronary artery disease (CAD) at rest and during exercise, producing maximum angina pectoris (AP). Left ventricular pressure was recorded simultaneously. The extent of CAD, demonstrated in all patients by coronary angiography, was quantitated by a score. In the exercise ventriculograms, local wall motion was quantitated by 14 hemiaxes. During exercise AP, all patients developed wall motion abnormalities not present at rest. There was a significant linear correlation between coronary score and number of abmormally shortening hemiaxes (< 30% shortening) during exercise‐AP (y = 0.16 × + 4.34; r = 0.933). The number of anormal hemiaxes correlated significantly (p < 0.05) with left ventricular enddiastolic pressure (LVEDP), dp/dt min, endsystolic volume index, enddiastolic volume index, ejection fraction, stroke work index, minute work, compliance SV/ Δ PD/ESV, and cardiac index. During exercise AP the extent of ischemic wall motion abnormalities is determined by localization and severity of coronary artery lesions. The extent of ischemic impairment of wall motion determines the severity of impairment of left ventricular pump function, filling pressure, and maximum speed of relaxation. Transpulmonary left ventriculography during exercise AP is a safe and relatively simple method to quantitate the extent of ischemic wall motion abnormalities. It could be useful in the selection of patients for coronary artery surgery and in the assessment of the results of this operation.
Left ventricular end‐diastolic pressure response to atrial pacing in idiopathic mitral valve prolapse
Authors: Feitosa G. S. , Bentivoglio L. G. , Uricchio J. F. , Meister S. G. , Frankl W. S. 
Published: October 2013

AbstractThe response of several parameters of left ventricular function to right atrial pacing was compared in 21 patients with idiopathic mitral valve prolapse and 10 normal patients. An inability to appropriately lower left ventricular end‐diastolic pressure with increasing rates was demonstrated in the mitral valve prolapse group. This abnormality was not related to mitral regurgitation or factors other than ventricular performance per se.
Pre‐infarction angina secondary to calcific aortic stenosis with bernheim's effect
Authors: Huang M. T. C. , Goodman M. A. , Delaney T. B. 
Published: October 2013

AbstractPre‐infarction angina, in the absence of coronary artery disease, was found in a 62 year‐old man with severe calcific aortic stenosis. After application of intra‐aortic balloon pump counter‐pulsation, the condition was stabilized, and coronary arteriograms were safely carried out. Interestingly, an elevated right atrial and right ventricular end‐diastolic pressure with an associated Bernheim's effect was demonstrated by cardiac catheterization. The hemodynamics of the right heart returned to normal after surgical correction of the aortic stenosis. The clinical indications for intra‐aortic balloon pump counterpulsation in this setting are discussed.
Technetium‐99m stannous pyrophosphate myocardial scintigraphy: Serial imaging to detect myocardial infarcts in patients
Authors: Falkoff M. , Parkey R. W. , Bonte F.J. , Lewis S. , Buja L.M. , Dehmer G. , Willerson J. T. 
Published: October 2013

AbstractTypically, technetium‐99m stannous pyrophosphate (99mTc‐PYP) myocardial scintigrams become abnormal within 10‐12 h following acute myocardial infarction. Ordinarily, the 99mTc‐PYP scintigrams become increasingly abnormal between 24 and 72 h following the infarct and then become normal 6‐7 days after the event. However, occasional patients and their 99mTc‐PYP myocardial scintigrams deviate from this expected pattern by initially developing an abnormal 99mTc‐PYP scintigram as late as 4‐5 days following an acute myocardial infarction. Others retain “persistently abnormal” 99mTc‐PYP myocardial scintigrams after their myocardial infarcts. The clinical importance and potential prognostic importance of these deviations from the expected patterns with 99mTc‐PYP myocardial imaging are discussed and the need for serial myocardial imaging is emphasized.
Noninvasive assessment of segmental left ventricular wall motion: Its clinical relevance in detection of ischemia
Authors: Pichler M. 
Published: October 2013

AbstractNewly developed noninvasive techniques, using fluoroscopic, electromagnetic, ultrasonic and nuclear medical devices, now make evaluation of segmental wall motion (SWM) widely applicable to man. Experiments in open chest animal preparations have demonstrated that SWM abnormalities (SWMA) are sensitive and specific markers of ischemia. Following coronary occlusion in closed chest dogs, using various techniques, we observed the same sequence of SWMA over a similar time course.In 122 angiographically documented patients, SWMA assessed by stress cardiokymography (CKG) revealed a sensitivity of 74% and a specificity of 94% in detecting coronary heart disease (CHD). The combined predictive power of stress ECG/CKG was superior to either test alone. Using radionuclide equilibrium scintigraphy and sitting bicycle exercise, stress induced SWMA revealed a sensitivity of 84% in detecting CHD.SWMA were detected in nearly all patients 24 h after acute transmural myocardial infarction (MI). These abnormalities extended beyond the location predicted by ST segment elevation and Q waves in the ECG, significantly more in anterior (15/16) than inferior (9/16) MI (p < 0.05). In inferior MI, precordial ST segment depression was frequently (8/10) associated with anterior SWMA, indicative of ischemia rather than of a reciprocal electrical phenomenon. Scintigraphic assessment of left ventricular SWM correlated poorly with calculated CK‐MB infarct size (r = 0.64), suggesting that left ventricular function is determined not only by the necrotic zone but is also related to dysfunction of ischemic segments. SWM of the infarcted as well as the remote zone of the left ventricle showed no consistent directional changes within 10 days after infarction.In conclusion, noninvasive analysis of SWM is of clinical relevance 1. during diagnostic stress testing to detect ischemia, 2. in chronic stable CHD to assess the extent of mechanical dysfunction, 3. in acute MI to localize and assess extent and severity of regional myocardial dysfunction.