bluuebunny/crossref_metadata_2025_split huggingface dataset:
An investigation into the sensory properties of luffa [Luffa cylindrica(L.)] sap
Authors: Gorman Mackenzie , Baxter Laura , Frampton Kaitlyn , Dabas Tanvi , Moss Rachael , McSweeney Matthew B. 
Published: June 2024

AbstractThe seeds and sap of luffa [Luffa cylindrica(L.)] are usually discarded as waste. As such, this study aimed to identify the sensory properties of luffa sap (aqueous solution) and if it can be incorporated into a food item (orange juice) for added nutritional benefits and as an alternative sweetener. A sensory trial (n = 94) asked participants to evaluate a luffa sap sample and five different orange juice samples with increasing amounts of luffa sap (control [0%], 5%, 7.5%, 10%, 12.5%). The participants evaluated the samples using 9‐point hedonic scales, check‐all‐that‐apply, and an open‐ended comment question. The luffa sap was described as having a mild flavor as well as woody, earthy, and floral attributes and an aftertaste. The liking scores for the orange juice with the 5% luffa sap did not significantly differ from the control. However, as the amount of luffa sap increased above 5%, the liking scores decreased and were significantly different from the control. The orange juice with luffa sap samples (7.5% and above) was associated with off‐flavors, while the orange juice with 5% luffa sap and the control were associated with the attributes (sweet, fruity, orange, tropical, citrus) that increased the participants liking. Future studies should continue to investigate the sensory properties of luffa sap and its incorporation into different food products.Practical ApplicationThis is one of the first studies to investigate the sensory properties of luffa sap with participants residing in the Western world. The luffa sap was found to be woody, earthy, bitter, and floral. It was acceptable to add luffa sap to orange juice up to 5% by volume. However, it did not increase the sweetness perception of the orange juice. At a 7.5% luffa sap addition and higher levels, off‐flavors were observed in the orange juice.
Inactivation of Pseudomonas putida KT2440 pyruvate dehydrogenase relieves catabolite repression and improves the usefulness of this strain for degrading aromatic compounds
Authors: Moreno Renata , Yuste Luis , Morales Gracia , Rojo Fernando 
Published: June 2024

AbstractPyruvate dehydrogenase (PDH) catalyses the irreversible decarboxylation of pyruvate to acetyl‐CoA, which feeds the tricarboxylic acid cycle. We investigated how the loss of PDH affects metabolism in Pseudomonas putida. PDH inactivation resulted in a strain unable to utilize compounds whose assimilation converges at pyruvate, including sugars and several amino acids, whereas compounds that generate acetyl‐CoA supported growth. PDH inactivation also resulted in the loss of carbon catabolite repression (CCR), which inhibits the assimilation of non‐preferred compounds in the presence of other preferred compounds. Pseudomonas putida can degrade many aromatic compounds, most of which produce acetyl‐CoA, making it useful for biotransformation and bioremediation. However, the genes involved in these metabolic pathways are often inhibited by CCR when glucose or amino acids are also present. Our results demonstrate that the PDH‐null strain can efficiently degrade aromatic compounds even in the presence of other preferred substrates, which the wild‐type strain does inefficiently, or not at all. As the loss of PDH limits the assimilation of many sugars and amino acids and relieves the CCR, the PDH‐null strain could be useful in biotransformation or bioremediation processes that require growth with mixtures of preferred substrates and aromatic compounds.
Pharmaceutical removal from wastewater by introducing cytochrome P450s into microalgae
Authors: Kariyawasam Thamali , Helvig Christian , Petkovich Martin , Vriens Bas 
Published: June 2024

AbstractPharmaceuticals are of increasing environmental concern as they emerge and accumulate in surface‐ and groundwater systems around the world, endangering the overall health of aquatic ecosystems. Municipal wastewater discharge is a significant vector for pharmaceuticals and their metabolites to enter surface waters as humans incompletely absorb prescription drugs and excrete up to 50% into wastewater, which are subsequently incompletely removed during wastewater treatment. Microalgae present a promising target for improving wastewater treatment due to their ability to remove some pollutants efficiently. However, their inherent metabolic pathways limit their capacity to degrade more recalcitrant organic compounds such as pharmaceuticals. The human liver employs enzymes to break down and absorb drugs, and these enzymes are extensively researched during drug development, meaning the cytochrome P450 enzymes responsible for metabolizing each approved drug are well studied. Thus, unlocking or increasing cytochrome P450 expression in endogenous wastewater microalgae could be a cost‐effective strategy to reduce pharmaceutical loads in effluents. Here, we discuss the challenges and opportunities associated with introducing cytochrome P450 enzymes into microalgae. We anticipate that cytochrome P450‐engineered microalgae can serve as a new drug removal method and a sustainable solution that can upgrade wastewater treatment facilities to function as “mega livers”.
Post‐traumatic lipid inclusion cyst
Authors: Rothe Christopher J , Sivakumar Brahman S , Graham David , Buchan Craig A 
Published: June 2024

SummaryPost‐traumatic lipid inclusion cysts are a rare entity seen following fractures in paediatric patients. They often occur in the distal radius, developing 1–3 months following an extra‐articular fracture. Although benign and self‐limiting in nature, adequate awareness and accurate radiographic interpretation is key in avoiding further unnecessary non‐invasive or invasive investigations. We report on a case of post‐traumatic lipid inclusion cyst and review the literature.
Geochemical and Isotopic Techniques Constraints on the Origin, Evolution, and Residence Time of Low‐enthalpy Geothermal Water in Western Wugongshan, SE China
Authors: WANG Luyao , LIU Kai , MA Yan , ZHANG Yaoyao , TONG Jue , JIA Wuhui , ZHANG Shouchuan , SUN Junliang 
Published: June 2024

AbstractGeothermal resources are increasingly gaining attention as a competitive, clean energy source to address the energy crisis and mitigate climate change. The Wugongshan area, situated in the southeast coast geothermal belt of China, is a typical geothermal anomaly and contains abundant medium‐ and low‐temperature geothermal resources. This study employed hydrogeochemical and isotopic techniques to explore the cyclic evolution of geothermal water in the western Wugongshan region, encompassing the recharge origin, water–rock interaction mechanisms, and residence time. The results show that the geothermal water in the western region of Wugongshan is weakly alkaline, with low enthalpy and mineralization levels. The hydrochemistry of geothermal waters is dominated by Na‐HCO3 and Na‐SO4, while the hydrochemistry types of cold springs are all Na‐HCO3. The hydrochemistry types of surface waters and rain waters are Na‐HCO3 or Ca‐HCO3. The δD and δ18O values reveal that the geothermal waters are recharged by atmospheric precipitation at an altitude between 550.0 and 1218.6 m. Molar ratios of major solutes and isotopic compositions of 87Sr/86Sr underscore the significant role of silicate weathering, dissolution, and cation exchange in controlling geothermal water chemistry. Additionally, geothermal waters experienced varying degrees of mixing with cold water during their ascent. The δ13C values suggest that the primary sources of carbon in the geothermal waters were biogenic and organic. The δ34S value suggests that the sulfates in geothermal water originate from sulfide minerals in the surrounding rock. Age dating using 3H and 14C isotopes suggests that geothermal waters have a residence time exceeding 1 kaBP and undergo a long‐distance cycling process.
Flat‐footed Females and Missing Males: A New Genus and Species of Pleciofungivoridae (Diptera: Bibionomorpha) Represents the First Record of the Family from Mid‐Cretaceous Kachin Amber
Authors: ZHANG Qingqing , KRZEMIŃSKI Wiesław , ŠEVČÍK Jan , BLAGODEROV Vladimir , SOSZYŃSKA Agnieszka , SKIBIŃSKA Kornelia 
Published: June 2024

AbstractA new genus and species, Cretopleciofungivora simpsoni gen. et sp. nov., from the extinct family Pleciofungivoridae (Diptera: Bibionomorpha), is discovered in mid‐Cretaceous Kachin amber. Previously, this family was known only from imprints in sedimentary rocks of the Jurassic and the Lower Cretaceous. Discovery of a representative of Pleciofungivoridae in Kachin amber confirms the presence of the family in the Upper Cretaceous. The new species has a unique structure of fore tarsus, with lobed and extended tarsal segments II to IV, a feature hitherto known only in a few species of extant Sciaroidea. Although not particularly rare, the new species is currently known only from female specimens. Possible reasons for this phenomenon, very unusual in Sciaroidea, are briefly discussed, including parthenogenesis as a potentially plausible hypothesis.
Exploring plastic biofilm formation and Escherichia coli colonisation in marine environments
Authors: Ballesté Elisenda , Liang Hongxia , Migliorato Laura , Sala‐Comorera Laura , Méndez Javier , Garcia‐Aljaro Cristina 
Published: June 2024

AbstractMicroorganisms, including potential pathogens, can colonise plastic surfaces in aquatic environments. This study investigates the colonisation of plastic pellets by Escherichia coli (E. coli) as a proxy for faecal pathogens in aquatic environments. Plastic pellets from a polluted beach were placed in seawater aquaria spiked with E. coli. Diverse bacteria, primarily from the Proteobacteria phylum, rapidly colonised the pellets within 24 h, with notable species known for plastic or hydrocarbon degradation. Over 26 days, biofilms formed on the plastic surfaces, reaching bacterial populations of up to 6.8·105 gene copies (gc) of the 16S rRNA mm−2. E. coli, was detected in the pellets for up to 7 days using culture methods, exhibiting varying attachment densities regardless of source or environmental factors. The study highlights plastic biofilms as reservoirs for E. coli, contributing to the survival and persistence of faecal bacteria in aquatic systems. These findings deepen our understanding of the risks associated with plastic pollution in marine settings, offering insights into the behaviour of faecal indicators and their implications for water quality assessments, while providing valuable information on potential pathogen dissemination within plastic‐associated microbial communities.
Single‐neuron analysis of aging‐associated changes in learning reveals impairments in transcriptional plasticity
Authors: Badal Kerriann K. , Sadhu Abhishek , Raveendra Bindu L. , McCracken Carrie , Lozano‐Villada Sebastian , Shetty Amol C. , Gillette Phillip , Zhao Yibo , Stommes Dustin , Fieber Lynne A. , Schmale Michael C. , Mahurkar Anup , Hawkins Robert D. , Puthanveettil Sathyanarayanan V. 
Published: June 2024

AbstractThe molecular mechanisms underlying age‐related declines in learning and long‐term memory are still not fully understood. To address this gap, our study focused on investigating the transcriptional landscape of a singularly identified motor neuron L7 in Aplysia, which is pivotal in a specific type of nonassociative learning known as sensitization of the siphon‐withdraw reflex. Employing total RNAseq analysis on a single isolated L7 motor neuron after short‐term or long‐term sensitization (LTS) training of Aplysia at 8, 10, and 12 months (representing mature, late mature, and senescent stages), we uncovered aberrant changes in transcriptional plasticity during the aging process. Our findings specifically highlight changes in the expression of messenger RNAs (mRNAs) that encode transcription factors, translation regulators, RNA methylation participants, and contributors to cytoskeletal rearrangements during learning and long noncoding RNAs (lncRNAs). Furthermore, our comparative gene expression analysis identified distinct transcriptional alterations in two other neurons, namely the motor neuron L11 and the giant cholinergic neuron R2, whose roles in LTS are not yet fully elucidated. Taken together, our analyses underscore cell type‐specific impairments in the expression of key components related to learning and memory within the transcriptome as organisms age, shedding light on the complex molecular mechanisms driving cognitive decline during aging.
Refusals of noncitizenship
Authors: Nyers Peter 
Published: June 2024

AbstractThis commentary explores the politics of refusal as it plays out in struggles for citizenship. Refusals of noncitizenship involve a dialectic of negation and affirmation. They are at once acts of protest against an injustice or wrong, while also generative of new forms of political subjectivity and community. The refusals of noncitizenship found in the articles of this special forum involve acts of world building that involve new rights, responsibilities, and communities in the making.
Exploring the Chinese public's affective attitudes towards digital transformation in agriculture: A social media‐based analysis
Authors: Wu Jinghua , Qiu Peng 
Published: June 2024

AbstractThis study utilizes natural language processing techniques and panel vector autoregression methodology, to delve into the perceived attitudes of social media users towards the digital transformation of agriculture, and to assess its impact on total agricultural output and agricultural science and technology inputs. Data related to agricultural digital transformation were collected from Sina Weibo using web crawlers. The SnowNLP model was employed to infer users' attitudes, encompassing both positive and negative aspects. Furthermore, the study delves into the specific themes capturing users' positive attitudes and explores regional variations in focus. The findings reveal a sustained increase in users' interest in agricultural digital transformation since 2013. Positive attitudes primarily center around green development, agricultural intelligence, and global cooperation and innovation. Moreover, the study establishes a significant positive impact of users' positive attitudes on both total agricultural output value and agricultural science and technology investment, highlighting the constructive influence of user support on the agricultural industry's development.
Recent infection with SARS‐CoV‐2 in donors was associated with a higher incidence of acute graft‐versus‐host disease in recipients undergoing allogeneic haematopoietic stem cell transplantation
Authors: Lin Fan , Xu Lanping , Han Tingting , Xu Zhengli , Liu Jing , He Yun , Chen Yao , Chen Huan , Han Wei , Chen Yuhong , Fu Haixia , Zhang Yuanyuan , Mo Xiaodong , Wang Fengrong , Wang Jingzhi , Cheng Yifei , Yan Chenhua , Sun Hui , Wang Yu , Zhang Xiaohui , Huang Xiaojun 
Published: June 2024

SummaryThe global pandemic has resulted in the common occurrence of SARS‐CoV‐2 infection in the population. In the post‐pandemic era, it is imperative to understand the influence of donor SARS‐CoV‐2 infection on outcomes after allogeneic haematopoietic stem cell transplantation (allo‐HSCT). We retrospectively analysed allo‐HSCTs from donors with mild SARS‐CoV‐2 infection or early recovery stage (ERS) (group 1, n = 65) and late recovery stage (group 2, n = 120). Additionally, we included allo‐HSCT from donors without prior SARS‐CoV‐2 infection as group 0 (n = 194). Transplants from donors with different SARS‐CoV‐2 infection status had comparable primary engraftment and survival rates. However, group 1 had higher incidences of acute graft‐versus‐host disease (aGvHD), grade II–IV (41.5% vs. 28.1% in group 0 [p = 0.014] and 30.6% in group 2 [p = 0.067]) and grade III–IV (22.2% vs. 9.6% [p = 0.004] in group 0 and 12.2% in group 2 [p = 0.049]). Conversely, the risk of aGvHD in group 2 was similar to that in group 0 (p > 0.5). Multivariable analysis identified group 1 associated with grade II–IV (hazard ratio [HR] 2.307, p = 0.010) and grade III–IV (HR 2.962, p = 0.001) aGvHD, which yielded no significant risk factors for survival. In conclusion, we preliminarily demonstrated donors in the active infection state or ERS of mild SARS‐CoV‐2 infection were associated with higher incidences of aGvHD in transplants from related donors.
Severe steroid‐related neuropsychiatric symptoms during paediatric acute lymphoblastic leukaemia therapy—An observational Ponte di Legno Toxicity Working Group Study
Authors: Anastasopoulou Stavroula , Swann Gemma , Andres‐Jensen Liv , Attarbaschi Andishe , Barzilai‐Birenboim Shlomit , Erdelyi Daniel J. , Escherich Gabriele , Hamadeh Lina , Harila Arja , Lopez‐Lopez Elixabet , McGowan Sheena , Möricke Anja , Putti Caterina , Sagi Judit C. , Schmiegelow Kjeld , Ullrich Nicole J. , van der Sluis Inge M. , Wahid Qurat‐ul‐Ain , Winick Naomi , Sramkova Lucie , Zalcberg Yair , Zapotocka Ester , Bhojwani Deepa , Halsey Christina ,   the Ponte Di Legno Neurotoxicity Working Group
Published: June 2024

SummarySteroids are a mainstay in the treatment of acute lymphoblastic leukaemia (ALL) in children and adolescents; however, their use can cause clinically significant steroid‐related neuropsychiatric symptoms (SRNS). As current knowledge on SRNS during ALL treatment is limited, we mapped the phenotypes, occurrence and treatment strategies using a database created by the international Ponte di Legno Neurotoxicity Working Group including data on toxicity in the central nervous system (CNS) in patients treated with frontline ALL protocols between 2000 and 2017. Ninety‐four of 1813 patients in the CNS toxicity database (5.2%) experienced clinically significant SRNS with two peaks: one during induction and one during intensification phase. Dexamethasone was implicated in 86% of SRNS episodes. The most common symptoms were psychosis (52%), agitation (44%) and aggression (31%). Pharmacological treatment, mainly antipsychotics and benzodiazepines, was given to 87% of patients while 38% were hospitalised due to their symptoms. Recurrence of symptoms was reported in 29% of patients and two previously healthy patients required ongoing pharmacological treatment at the last follow up. Awareness of SRNS during ALL treatment and recommendation on treatment strategies merit further studies and consensus.
Real‐world performance of Uromonitor® in urothelial bladder cancer detection: a multicentric trial
Authors: Wolff Ingmar , Kravchuk Anton P. , Wirtz Ralph M. , Schlomm Thorsten , Rabien Anja , Rong Dezhi , Hofbauer Sebastian L. , Labonté Flora K. , Barski Dimitri , Otto Thomas , Gössl Andreas , Brookman‐May Sabine D. , Gilfrich Christian P. , Ecke Thorsten H. , May Matthias 
Published: June 2024

ObjectivesTo compare Uromonitor® (U‐Monitor Lda, Porto, Portugal), a multitarget DNA assay that detects mutated proto‐oncogenes (telomerase reverse transcriptase [TERT], fibroblast growth factor receptor 3 [FGFR‐3], Kirsten rat sarcoma viral oncogene homologue [KRAS]), with urine cytology in the urine‐based diagnosis of urothelial carcinoma of the bladder (UCB) within a multicentre real‐world setting.Patients and MethodsThis multicentre, prospective, double‐blind study was conducted across four German urological centres from 2019 to 2024. We evaluated the diagnostic performance of Uromonitor compared to urine cytology in a cohort of patients with UCB and in healthy controls within a real‐world setting. Sensitivity, specificity, positive‐predictive value (PPV), negative‐predictive value (NPV), and accuracy of the tests were measured, in addition to multivariate analyses to assess the ability of individual proto‐oncogene mutations in detecting UCB. The biometric sample size was designed to achieve a 10% difference in sensitivity.ResultsPatients with UCB comprised 63.7% (339/532) of the study group. Uromonitor showed a sensitivity, specificity, PPV, NPV, accuracy, and an area‐under‐the‐curve of 49.3%, 93.3%, 92.8%, 51.1%, 65.2%, and 0.713%, respectively. These metrics did not demonstrate statistical superiority over urine cytology in terms of sensitivity (44.6%; P = 0.316). Moreover, the comparison of additional test parameters, as well as the comparison within various sensitivity analyses, yielded no significant disparity between the two urinary tests. Multivariate logistic regression underscored the significant predictive value of a positive Uromonitor for detecting UCB (odds ratio [OR] 9.03; P < 0.001). Furthermore, mutations in TERT and FGFR‐3 were independently associated with high odds of UCB detection (OR 13.30 and 7.04, respectively), while KRAS mutations did not exhibit predictive capability.ConclusionDespite its innovative approach, Uromonitor fell short of confirming the superior results anticipated from previous studies in this real‐world setting. The search for an optimal urine‐based biomarker for detecting and monitoring UCB remains ongoing. Results from this study highlight the complexity of developing non‐invasive diagnostic tools and emphasise the importance of continued research efforts to refine these technologies.
The contribution of NaV1.6 to the efficacy of voltage‐gated sodium channel inhibitors in wild type and NaV1.6 gain‐of‐function (GOF) mouse seizure control
Authors: Johnson James P. , Focken Thilo , Karimi Tari Parisa , Dube Celine , Goodchild Samuel J. , Andrez Jean‐Christophe , Bankar Girish , Burford Kristen , Chang Elaine , Chowdhury Sultan , Christabel Jessica , Dean Richard , de Boer Gina , Dehnhardt Christoph , Gong Wei , Grimwood Michael , Hussainkhel Angela , Jia Qi , Khakh Kuldip , Lee Stephanie , Li Jenny , Lin Sophia , Lindgren Andrea , Lofstrand Verner , Mezeyova Janette , Nelkenbrecher Karen , Shuart Noah Gregory , Sojo Luis , Sun Shaoyi , Waldbrook Matthew , Wesolowski Steven , Wilson Michael , Xie Zhiwei , Zenova Alla , Zhang Wei , Scott Fiona L. , Cutts Alison J. , Sherrington Robin P. , Winquist Raymond , Cohen Charles J. , Empfield James R. 
Published: June 2024

Background and PurposeInhibitors of voltage‐gated sodium channels (NaVs) are important anti‐epileptic drugs, but the contribution of specific channel isoforms is unknown since available inhibitors are non‐selective. We aimed to create novel, isoform selective inhibitors of Nav channels as a means of informing the development of improved antiseizure drugs.Experimental ApproachWe created a series of compounds with diverse selectivity profiles enabling block of NaV1.6 alone or together with NaV1.2. These novel NaV inhibitors were evaluated for their ability to inhibit electrically evoked seizures in mice with a heterozygous gain‐of‐function mutation (N1768D/+) in Scn8a (encoding NaV1.6) and in wild‐type mice.Key ResultsPharmacologic inhibition of NaV1.6 in Scn8aN1768D/+ mice prevented seizures evoked by a 6‐Hz shock. Inhibitors were also effective in a direct current maximal electroshock seizure assay in wild‐type mice. NaV1.6 inhibition correlated with efficacy in both models, even without inhibition of other CNS NaV isoforms.Conclusions and ImplicationsOur data suggest NaV1.6 inhibition is a driver of efficacy for NaV inhibitor anti‐seizure medicines. Sparing the NaV1.1 channels of inhibitory interneurons did not compromise efficacy. Selective NaV1.6 inhibitors may provide targeted therapies for human Scn8a developmental and epileptic encephalopathies and improved treatments for idiopathic epilepsies.