bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ardisia crispa roots inhibit cyclooxygenase and suppress angiogenesis
Authors: Awang Hamsin Dayang Erna Zulaikha , Abdul Hamid Roslida , Saiful Yazan Latifah , Mat Taib Che Norma , Yeong Looi Ting 
Published: March 2014

AbstractBackgroundIn our previous studies conducted onArdisia crisparoots, it was shown thatArdisia crisparoot inhibited inflammation-induced angiogenesisin vivo. The present study was conducted to identify whether the anti-angiogenic properties ofArdisia crisparoots was partly due to either cyclooxygenase (COX) or/and lipoxygenase (LOX) activity inhibition in separate in vitro studies.MethodsBenzoquinonoid fraction (BQ) was isolated from hexane extract by column chromatography, and later analyzed by using gas chromatography–mass spectrometry (GC-MS). Anti-angiogenic effect was studied on mouse sponge implantation assay.Ardisia crispaethanolic rich fraction (ACRH), quinone-rich fraction (QRF) and BQ were screened for COX assay to evaluate their selectivity towards two isoforms (COX-1 and COX-2), The experiment on soy lipoxygenase (LOX) inhibitory assay was also performed to determine the inhibitory effect of ACRH, QRF and BQ on soy LOX.ResultsBQ was confirmed to consist of 2-methoxy-6-undecyl-1,4-benzoquinone, when compared with previous data. Antiangiogenesis study exhibited a reduction of mean vascular density (MVD) in both ACRH and QRF, compared to control. In vitro study showed that both ACRH and QRF inhibited both COX-1 and COX-2, despite COX-2 inhibition being slightly higher than COX-1 in BQ. On the other hand, both ACRH and QRF were shown to have poor LOX inhibitory activity, but not BQ.ConclusionsIn conclusion, ACRH and QRF might possibly exhibit its anti-angiogenic effect by inhibiting cyclooxygenase. However, both of them were shown to possess poor LOX inhibitory activity. On the other hand, BQ displayed selectivity to COX-2 inhibitory property as well as LOX inhibitory effect.
The utilisation of Complementary and Alternative Medicine (CAM) among ethnic minorities in South Korea
Authors: Hwang Jung Hye , Han Dong Woon , Yoo Eun Kyung , Kim Woon-Yong 
Published: March 2014

AbstractBackgroundRace has been reported to affect the use of complementary and alternative medicine (CAM), but there is very little research on the use of CAM by ethnicity in Korea. This study explores the prevalence of CAM use among ethnic minorities in South Korea.MethodsThe design is a descriptive and cross-sectional study. A convenience sample of ethnic minorities was recruited from two public healthcare centres in Gyeonggi province. The survey instrument included 37 questions regarding CAM use, factors influencing use of CAM, self-health management, and the socio-demographic profile of study participants.ResultsSixty-two percent of study participants reported the use of CAM. Multivitamins (53.3%), acupuncture (48.9%), and traditional Korean herbal medicine (38.9%) were popular CAM modalities in our sample. Other notable CAM modalities included herbal plants, therapeutic massage, and moxibustion therapy. The majority of CAM users (52.2%) received CAM services to treat diseases or as a secondary treatment while receiving conventional care. Having positive perceptions toward the effectiveness of CAM was a major determining factor in CAM use.ConclusionsPhysicians need to be aware of the fact that many ethnic minorities use CAM therapies. Many CAM users reported that they want doctors to know about their CAM use and have a basic understanding of traditional medicine in their home country. Overcoming language and cultural barriers will help reduce unwanted medical complications. High prevalence of CAM use among ethnic minorities in our study warrants further studies using larger sample population.
Variation in the location of the shoe sole flexion point influences plantar loading patterns during gait
Authors: van der Zwaard Babette C , Vanwanseele Benedicte , Holtkamp Fred , van der Horst Henriëtte E , Elders Petra JM , Menz Hylton B 
Published: March 2014

AbstractBackgroundSeveral footwear design characteristics are known to have detrimental effects on the foot. However, one characteristic that has received relatively little attention is the point where the sole flexes in the sagittal plane. Several footwear assessment forms assume that this should ideally be located directly under the metarsophalangeal joints (MTPJs), but this has not been directly evaluated. The aim of this study was therefore to assess the influence on plantar loading of different locations of the shoe sole flexion point.MethodTwenty‐one asymptomatic females with normal foot posture participated. Standardised shoes were incised directly underneath the metatarsophalangeal joints, proximal to the MTPJs or underneath the midfoot. The participants walked in a randomised sequence of the three shoes whilst plantar loading patterns were obtained using the Pedar® in‐shoe pressure measurement system. The foot was divided into nine anatomically important masks, and peak pressure (PP), contact time (CT) and pressure time integral (PTI) were determined. A ratio of PP and PTI between MTPJ2‐3/MTPJ1 was also calculated.ResultsWearing the shoe with the sole flexion point located proximal to the MTPJs resulted in increased PP under MTPJ 4–5 (6.2%) and decreased PP under the medial midfoot compared to the sub‐MTPJ flexion point (−8.4%). Wearing the shoe with the sole flexion point located under the midfoot resulted in decreased PP, CT and PTI in the medial and lateral hindfoot (PP: −4.2% and −5.1%, CT: −3.4% and −6.6%, PTI: −6.9% and −5.7%) and medial midfoot (PP: −5.9% CT: −2.9% PTI: −12.2%) compared to the other two shoes.ConclusionThe findings of this study indicate that the location of the sole flexion point of the shoe influences plantar loading patterns during gait. Specifically, shoes with a sole flexion point located under the midfoot significantly decrease the magnitude and duration of loading under the midfoot and hindfoot, which may be indicative of an earlier heel lift.
Is intra-abdominal hypertension a missing factor that drives multiple organ dysfunction syndrome?
Authors: Kirkpatrick Andrew W , Roberts Derek J , De Waele Jan , Laupland Kevin 
Published: March 2014

AbstractIn a recent issue of Critical Care, Cheng and colleagues conducted a rabbit model study that demonstrated that intra-abdominal hypertension (IAH) may damage both gut anatomy and function. With only 6 hours of IAH at 25 mmHg, these authors observed an 80% reduction in mucosal blood flow, an exponential increase in mucosal permeability, and erosion and necrosis of the jejunal villi. Such dramatic findings should remind all caring for the critically ill that IAH may severely damage the normal gut barrier functions and thus may be reasonably expected to facilitate bacterial and mediator translocation. The potential contribution of IAH as a confounding factor in the efficacy of selective decontamination of the digestive tract should be considered.
Cloning, expression and characterization of a versatile Baeyer-Villiger monooxygenase from Dietzia sp. D5
Authors: Bisagni Serena , Hatti-Kaul Rajni , Mamo Gashaw 
Published: March 2014

Abstract
A novel BVMO encoding gene was identified from a draft genome sequence of a newly isolated strain of Dietzia. Analysis of the protein sequence revealed that it belongs to a group of BVMOs whose most characterized member is cyclopentadecanone monooxygenase (CPDMO). The gene was PCR amplified, cloned and successfully expressed in E. coli. The expressed recombinant enzyme was purified using metal affinity chromatography. Characterization of the purified enzyme revealed that it has a broad substrate scope and oxidized different compounds including substituted and unsubstituted alicyclic, bicyclic-, aliphatic-ketones, ketones with an aromatic moiety, and sulfides. The highest activities were measured for 2- and 3-methylcyclohexanone, phenylacetone, bicyclo-[3.2.0]-hept-2-en-6-one and menthone. The enzyme was optimally active at pH 7.5 and 35°C, a temperature at which its half-life was about 20 hours. The stability studies have shown that this enzyme is more stable than all other reported BVMOs except the phenylacetone monooxygenase from the thermophilic organism Thermobifida fusca.
Comparison of articular cartilage repair with different hydrogel-human umbilical cord blood-derived mesenchymal stem cell composites in a rat model
Authors: Chung Jun Young , Song Minjung , Ha Chul-Won , Kim Jin-A , Lee Choong-Hee , Park Yong-Beom 
Published: March 2014

Abstract
Introduction
The present work was designed to explore the feasibility and efficacy of articular cartilage repair using composites of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) and four different hydrogels in a rat model.

Methods
Full-thickness articular cartilage defects were created at the trochlear groove of femur in both knees of rats. Composites of hUCB-MSCs and four different hydrogels (group A, 4% hyaluronic acid; group B, 3% alginate:30% pluronic (1:1, v/v); group C, 4% hyaluronic acid: 3% alginate: 20% pluronic (2:1:1, v/v}; and group D, 4% hyaluronic acid:3% alginate:20% pluronic;chitosan (4:1:1:2, v/v).) were then transplanted into right knee defect in each study group (five rats/group). Left knees were transplanted with corresponding hydrogels without hUCB-MSCs as controls. At 16 weeks post-transplantation, degrees of cartilage repair were evaluated macroscopically and histologically using Masson’s Trichrome, safranin-O, Sirius red staining, and type-II collagen immunostaining.

Results
Overall, group A with 4% hyaluronic acid hydrogel resulted in superior cartilage repair grossly and histologically and achieved a cellular arrangement and collagen organization pattern mimicking adjacent uninjured articular cartilage. Immunostaining and safranin-O staining also revealed that group A displayed the largest areas of type II collagen staining. Sirius red staining revealed that the organization pattern of collagen bundles was more similar to normal cartilage in group A. No evidence of rejection was found.

Conclusions
The results of this study suggest that hUCB-MSCs could be used to repair articular cartilage defects in vivo and that hyaluronic acid is an attractive hydrogel candidate for use in combination with hUCB-MSCs.
Wnt-Independent Role of β-Catenin in Thyroid Cell Proliferation and Differentiation
Authors: Sastre-Perona Ana , Santisteban Pilar 
Published: March 2014

Abstract
The Wnt/β-catenin pathway has been associated with thyroid cell growth and tumorigenesis. However, little is known regarding its involvement in the response to the key regulators of thyroid cell proliferation and differentiation. Here we show that TSH and IGF-1 increase β-catenin nuclear accumulation and its transcriptional activity in differentiated thyroid cells. This effect takes place in a Wnt-independent manner because TSH and IGF-1, through the activation of protein kinase A and protein kinase B/Akt, phosphorylate β-catenin at S552 and S675, which results in β-catenin release from E-cadherin at the adherens junctions. Nuclear β-catenin regulates thyroid cell proliferation, because its silencing or the overexpression of a dominant-negative form of T-cell factor 4 resulted in reduced levels of cyclin D1 and DNA synthesis. Furthermore, the β-catenin silencing markedly reduced the expression of Pax8, the main transcription factor involved in epithelial thyroid cell differentiation. Finally, we observed that β-catenin physically interacts with the transcription factor Pax8, increasing its transcriptional activity on the sodium iodide symporter (NIS) gene, a critical gene required for thyroid cell physiology. Taken together, our findings show that β-catenin plays a not yet described role in thyroid function including a functional interaction with Pax8.
An Adaptive De-Noising Method via the Lifting Scheme
Authors: Jing-Bin Wang , Bao Wang 
Published: March 2014

The wavelet transform de-noising method based on the threshold shrinkage is widely used, however, the classical threshold shrinkage method maybe exists constant deviation or appears additional concussion after reconfiguration. If the threshold value is chosen too large, part of useful signal points will be eliminated; if chosen too small, part of the noise will be kept. In addition, the “first generation wavelet” de-noising method only can choose one type of wavelet base, and can't be changed during the de-noising process. Due to the different wavelet has its own characteristics and applicable signal, It can achieve local optimum then overall optimization that choosing wavelet in a small wave focus according to the partial feature of the signal, namely using the multiwavelets to comprehensively decompose signal. This paper puts forward a new threshold function, then improves the selection scheme of threshold value, achieves that it can select dynamically according to the wavelet decomposition levels; Finally, on the basis of the lifting theory, this paper puts forward an adaptive method, which can select wavelet dynamically according to the partial feature of the signal, comprehensively making use of the advantages of each wavelet, to achieve better de-noising effect and a certain degree of adaptability. Experiments show the effectiveness of our optimization.
An Overview Of Optimising Passive Fire Protection Using A Limiting Temperature By Structural Assessment
Authors: Scott Peter 
Published: March 2014

The British Standards and the Eurocodes both cover the design of steel structures in fire. These provide designers with the opportunity to exploit the properties of structural steel to its maximum capacity in the fire limit state, termed Structural Fire Engineering. This allows fire protection measures to be integrated into structural design as part of the passive fire protection specification. If used effectively in the specification process it can bring significant benefits to the project, including robust and safe designs, quantified structural performance and cost savings.The suitability of a member in a structural design is generally governed by serviceability limitations such as deflection. Generally this approach provides a conservative working stress for the steel sections of approximately 50% of their overall capacity. Within the UK, this simplified approach led to the development of prescribed limiting steel temperatures of 550°C for columns and 620°C for beams.Best-practice industry guidance published in the UK encourages designers to specify a limiting steel temperature together with the fire resistance period as part of the overall steelwork specification. Passive fire protection is generally specified post design-stage at a contractor or applicator level and sometimes very little is known about the limiting steel temperature.This paper provides an overview of the fire protection specification and provides advice on structural fire engineering to determine a limiting steel temperature and the benefits that can be gained from doing so. The paper highlights how supplier expertise in fire engineering approaches and product performance knowledge can bring value to a design and play an important role in providing safe, cost effective and compliant solutions.
A novel variable delay Go/No-Go task to study attention, motivation and working memory in the head-fixed rodent
Authors: Dolzani Samuel D , Nakamura Shinya , Cooper Donald C 
Published: March 2014

In order to parse the causal elements underlying complex behaviors and decision-making processes, appropriate behavioral methods must be developed and used in concurrence with molecular, pharmacological, and electrophysiological approaches. Presented is a protocol for a novel Go/No-Go behavioral paradigm to study the brain attention and motivation/reward circuitry in awake, head-restrained rodents. This experimental setup allows: (1) Pharmacological and viral manipulation of various brain regions via targeted guide cannula; (2) Optogenetic cell-type specific activation and silencing with simultaneous electrophysiological recording and; (3) Repeated electrophysiological single and multiple unit recordings during ongoing behavior. The task consists of three components. The subject first makes an observing response by initiating a trial by lever pressing in response to distinctive Go or No-Go tones. Then, after a variable delay period, the subject is presented with a challenge period cued by white noise during which they must respond with a lever press for the Go condition or withhold from lever pressing for the duration of the cue in the No-Go condition. After correctly responding during the challenge period (Challenge) and a brief delay, a final reward tone of the same frequency as the initiation tone is presented and sucrose reward delivery is available and contingent upon lever pressing. Here, we provide a novel procedure and validating data set that allows researchers to study and manipulate components of behavior such as attention, motivation, impulsivity, and reward-related working memory during an ongoing operant behavioral task while limiting interference from non task-related behaviors.
Whether There Were Changes in Lesson Structure After Introduction of FSES of PGE?
Authors: Виноградова  , Vinogradova Natalya 
Published: March 2014

Main elements of lesson structure that meets requirements of the Federal State Educational Standard of the Primary General Education (FSES
of PGE) and corresponds to new tasks set for modern school are revealed in this paper. The special attention has been paid to planned personal,
meta subject and subject results which introduction assumes a search of new methodical approaches to junior school pupils’ teaching organization,
changes in traditional lesson structure, and in the nature of interaction between teacher and pupils.
Junior school pupils’ metasubject results
Authors: Кузнецова  , Kuznetsova Marina 
Published: March 2014

Models related to current and total estimation of junior school pupils’ meta subject results achievement are considered in this paper, and each
model’s features are proved. Possibilities of pedagogical diagnostics in estimation of meta subject results are discussed in detail. The offered
material allows a teacher to choose the most adequate models of assessment proceeding from specifics of meta subject results themselves.
&#34;Foundations of religious cultures and secular ethics&#34; course realization (from the experience of schools in the Republic of Bashkortostan)
Authors: Пичугин  , Pichugin Sergey 
Published: March 2014

In this paper an actual problem of modern primary education — &#34;Bases of Religious Cultures and Secular Ethics&#34; course introduction in the
curriculum is considered. The author notes difficulties and contradictions which had appeared at the first stage of this course studying at general
primary school, and describes possible ways of their elimination.
Obsolete words dictionary creation by the 4th Grade pupils at literary reading lessons
Authors: Дятлова  , Dyatlova N. 
Published: March 2014

Primary school pupils’ creative work related to a project on obsolete words dictionary creation is revealed in this paper. A characteristic of main
methods and techniques related to development of primary school pupils’ design activity is given, management features of primary school pupils’
design activity which is organized taking into account their age, are considered.
Cloudbasierte Infrastruktur für Bibliotheksdaten – Kernelemente und aktueller Entwicklungsstand
Authors: Risch Uwe 
Published: March 2014

Zusammenfassung:
Das Projekt „Cloudbasierte Infrastruktur für Bibliotheksdaten“ (CIB) der deutschen Bibliotheksverbünde HeBIS, BVB und KOBV zielt auf eine Reform der hergebrachten Arbeitsteilung von lokalem Bibliotheksmanagementsystem und regionalem Verbundsystem. An ihre Stelle tritt die weitgehende Verlagerung von Katalogisierung und Lokalsystemfunktionen in internationale Plattformen. Der Aufsatz stellt die Kernelemente dieses Vorhabens dar und beschreibt den aktuellen Entwicklungsstand des Projektes.
Gesetzliche Regelungen zur Förderung von Open Access – ein kurzer Problemaufriss anlässlich eines neuen argentinischen Gesetzes
Authors: Steinhauer Eric W. 
Published: March 2014

Zusammenfassung:
Es werden unterschiedliche Wege aufgezeigt, Open Access mit rechtlichen Instrumenten zu fördern. Besonders hervorgehoben werden dabei aktuelle Bestrebungen, durch eine entsprechende gesetzliche Verpflichtung für von der öffentlichen Hand bezahlte Wissenschaftler die Zahl der frei zugänglichen wissenschaftlichen Veröffentlichungen zu erhöhen. Als Beispiel für eine solche Verpflichtung wird ein jüngst in Argentinien erlassenes Gesetz in einer Übersetzung vorgestellt.
Bibliotheken als Bildungspartner
Authors: Marci-Boehncke Gudrun 
Published: March 2014

Zusammenfassung:
Die Rolle der Bibliotheken bei der Leseförderung geht über die Bereitstellung von Texten und Medien hinaus. Eine wichtige Rolle spielen pädagogische Überlegungen, wie diejenigen Kinder und Jugendliche zu erreichen sind, die aus lesefernen Elternhäusern stammen. Schulen und Kitas allein können momentan diese Kinder noch nicht zuverlässig für die Nutzung von Bibliotheken interessieren. Über intensive Bildungskooperationen soll es gelingen, den Ort Bibliothek für Kinder auch in Kita und Schulalltag präsenter werden zu lassen. Bibliotheken stehen dabei an der Schnittstelle zwischen institutionell bestimmtem und privatem Alltag. Sie können zu einer stabilisierenden Institution in der Bildungsbiographie von Heranwachsenden werden, wenn es gelingt, diese Verantwortung als gesellschaftliches Gut im Bewusstsein der Bibliotheken und der breiten – auch politischen – Öffentlichkeit zu verankern. Dazu muss auch die wechselseitige Kenntnis der Möglichkeiten und Bedarfe zwischen den Institutionen optimiert werden. Ein Vergleich mit Finnland zeigt, dass es zur Intensivierung der Nutzung immer noch Menschen bedarf, die in Bibliotheken beraten und das Lesen moderieren.
Latitudinal, seasonal, and small-scale spatial differences of the giant kelp, Macrocystis pyrifera, and an herbivore at their southern range limit in the northern hemisphere
Authors: Beas-Luna Rodrigo , Ladah Lydia B. 
Published: March 2014

Abstract
Although several studies have described kelp-urchin interactions, little is known about these interactions toward the distributional range limits of these species in areas that may experience different physical conditions than at the center of the distributional range. We explored seasonal and latitudinal changes in the population structure of the giant kelp and the purple sea urchin at their southern limits in the northern hemisphere. Densities of both organisms were positively correlated, showing a significant effect of season and latitude, and were highest in the temperate kelp populations and in the spring. The near subtropical kelp populations, however, contained significantly larger urchins. Along the boundary between the kelp beds and the urchin barrens studied, no significant spatial difference was found in sea urchin size or density. However, a general pattern of increasing probability of urchin presence with distance outside of the bed was found in spring at all sites. Spring bottom temperatures were generally colder and more variable in the temperate kelp populations, potentially explaining the larger seasonal variability found there. Data suggest seasonal differences may be modulated by the environmental variability in the temperate kelp populations and support local coupling of these two organisms at their southern range limit, in spite of the particularly unique physical conditions which occur in these populations at times.