bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pharmacognostic Evaluation, Phytochemical Screening and Anti-Candidal Activities of Leaves of Some Medicinal Plants against <i>Candida albicans</i>
Authors: Udebunu Onyinye A. , Sawa Fatima B. J. , Yusuf Zikrullah A. , Kolo Ibrahim 
Published: July 2024

Three Nigerian medicinal plants Psidium guajava Linn, Senna siamea Lam, and Senna obtusifolia L were investigated to determine the pharmacognostic characteristics, phytochemical contents and their activities against Candida albicans. The fresh leaves of each plant were examined for their microscopic characters and then processed. The dried ground leaves were extracted using water and ethanol. The extracts were subjected to phytochemical screening and their anticandida activities were determined using paper disc diffusion method at concentrations of 25mg/ml; 50mg/ml; 100mg/ml; 200mg/ml and 400mg/ml with Ketoconazole as control. The study indicated that the plant extracts contained alkaloids, anthraquinones, flavonoids, saponins, steroids, tannins and terpenoids. Glycoside was found to be absent. The extracts of the plants showed varying mean zones of inhibition (MZI) against C. albicans ranging from 6.00±0.00 to 16.67±1.53mm. The Minimum Inhibitory Concentration (MIC) of the extracts ranged from 10mg/ml to 20mg/ml while the Minimum Fungicidal Concentration (MFC) ranged from 20mg/ml to >80mg/ml with P. guajava having the least MIC and MFC of 10mg/ml and 20mg/ml respectively. The results indicated that MZI of the aqueous and ethanol extracts of P. guajava at a concentration of 400mg/ml had no significant differences at p ≤ 0.05. Further research is recommended for possible combinations of lower doses of the extracts to study their synergistic effects on C. albicans and to explore other potentials of the plants against other pathogens.
PARTISIPASI MASYARAKAT DALAM PENINGKATAN WISATA SUNGAI PERAHU KALIMAS SEBAGAI UPAYA PENGEMBANGAN WISATA HERITAGE DI KOTA SURABAYA
Authors: Kurniawan Ilham Yusuf , Rochim Achluddin Ibnu , Murti Indah 
Published: July 2024

Pengembangan suatu objek wisata heritage tidaklah lepas dari partisipasi masyarakat didalamnya, salah satu objek wisata yang perlu dikembangkan terus menerus ialah Wisata Perahu Kalimas yang berada di pusat kota Surabaya. Sungai kalimas merupakan sungai yang bersejarah pada masa jaman belanda. Seiring bertambahnya zaman sungai kalimas berangsur dijadikan objek wisata perahu yang dimana masyarakat bisa menikmati keindahan Kota Surabaya dan juga bisa melihat bangunan bersejarah disekitaran sungai kalimas. Penelitian ini membahas mengenai partsisipasi masyarakat dalam peningkatan wisata sungai perahu kalimas sebagai upaya pengembangan wisata heritage di kota surabaya. Tujuan dari penelitian ini adalah untuk mengidentifikasi bentuk partisipasi masyarakat dalam peningkatan wisata sungai perahu kalimas sebagai upaya pengembangan wisata heritage di kota surabaya. Teori yang digunakan ialah teori partisipasi dari Cohen dan Uphof 1977 yang dimana ada 4 tahapan dalam partisipasi yaitu partisipasi dalam perencaanan, partisipasi dalam pelaksanaan,partisipasi dalam pemanfaatan hasil dan partisipasi dalam pengawasan dan evaluasi. Metode yang digunakan dalam penelitian yaitu metode kualitatif deskriptuf dengan pendekatan wawancara, observasi dan dokumentasi. Hasil dari penelitian ini adalah bahwa partisipasi masyarakat dalam pengembangan wisata perahu kalimas sudah sangat baik terlihat dengan peningkatan jumlah pengunjung wisata setiap tahunnya, namun masyarakat disini hanya berperan sebag pelaku wisata. Dalam 4 tahapan partisipasi terlihat bahwa masyarakat tidak terlibat dalam hal pelaksanaan masyarakat hanya berperan sebagai pelaku wisata bukan pengelola objek wisata.
Ohdo-Madokoro-Sonoda Syndrome with a De Novo MED12 Mutation
Authors: Baidi H. , Radi A. , Ourrai A. , Hassani A. , Abilkassem R. 
Published: July 2024

Ohdo syndrome is extremely rare and comprises a heterogeneous group of disorders characterized by intellectual disability (ID) and typical facial features, including blepharophimosis, ptosis, dental hypoplasia, hearing impairment and intellectual disability. So far, fewer than 30 patients have been reported with Ohdo syndrome, with a prevalence of 1/1 000 000. Most reported cases are sporadic, except the original cases of ohdo who described two affected sisters and a first cousin, suggesting autosomal recessive inheritance. Autosomal dominant, X-linked- and mithochondrial inheritance have also been suggested.
We report the case of a 5-year-old girl, born to healthy, non-consanguineous parents, with no other family members known to be affected by a similar disorder. She exhibited significant dysmorphic facial features, including blepharophimosis, dental hypoplasia, hypertélorism, rétrognatism, microcephaly, trigonocéphaly, microphthalmia, nasolabial furrow, badly hemmed ear, and a pointed palate. These features were associated with psychomotor and growth retardation of less than 2 Standard deviations. Using whole exome sequencing (WES), we discovered the variant NM_005120.3 (MED12):c.6352C>T(p.Gin2118Ter) in the heterozygous state and its absence in the parents, confirming the de novo nature of this variant, which is compatible with the diagnosis of ohdo syndrome due to a heterozygous mutation of the MED 12.
Vertical Farming: Way for Urban Food Security
Authors: Vaishnavi.  , M. S. Udaykumar, , Patil Manojkumar , L. Suman, 
Published: July 2024

Agriculture plays a vital role in supporting the world's population, but it faces challenges like declining water availability and ecological problems caused by concentrated traditional farming. As cities grow and demand for food rises, there is a need for new agricultural methods. Vertical farming is an emerging technology that offers a promising solution to the challenges of global food security. Its controlled conditions eliminate the need for excessive fertilizers and pesticides, resulting in significantly increased yields compared to conventional farming. As the world's population continues to grow and urbanization increases, the demand for food in cities rises, making smart urban-vertical farming an attractive option. The technology's efficiency in land and water usage, along with reduced transportation and storage costs, enhances its economic viability. Moreover, regions facing threats from soil degradation and water scarcity, can benefit from vertical farming's potential water savings of up to 95 per cent and land productivity twice as high as traditional agriculture. The global vertical farming market is projected to grow, offering a promising tool for sustainable food production and resilient urban environments. Vertical farming presents a novel approach to address the challenges of food production and sustainability in the coming decades, fostering a balanced coexistence with nature while meeting the needs of a growing global population. By embracing vertical farming alongside other sustainable agricultural practices, can meet the global food security in future.
Impact of AI based Irrigation Scheduling Approaches and Drip Irrigation Methods on Yield of Chilli (Capsicum annum L.) and Chemical Properties of Soil
Authors: Bhavitha K. , Pasha Md. Latheef , Ramulu V. , Prakash T. Ram , Rajaiah P. , Revathi P. 
Published: July 2024

Aim: To assess the effect of AI based irrigation scheduling approaches and drip irrigation methods on soil chemical properties and yield in chilli.
Study Design: The study employs drip irrigation methods as the main plots and irrigation scheduling approaches as the subplots. A split plot design was chosen as suitable design because the main plots (drip irrigation methods) need a bigger plot sizes and subplots (irrigation scheduling approaches) requires more precise results with smaller plot sizes.
Place and Duration of Study: Water Technology Centre field, College Farm, College of Agriculture, Rajendranagar, Hyderabad during rabi 2022-23 (first year) and 2023-24 (second year).
Methodology: The investigation consisted of two drip irrigation methods as main plots and four irrigation scheduling approaches as subplots with total of 8 treatment combinations replicated thrice. Data recorded on various parameters was subjected to scrutiny by ANOVA technique for split plot design concept.
Results: Green (fresh) fruit and stalk yield was found to be significantly higher under subsurface drip (41859 and 5037 kg ha-1) among drip irrigation methods; whereas, among irrigation scheduling approaches, ET sensor based irrigation triggering resulted in significantly higher green (fresh) fruit and stalk yield (43139 and 5196 kg ha-1) followed by irrigation scheduling at 1.0 Epan by manual (control) (42235 and 5065 kg ha-1). The post-harvest soil chemical properties were found to be non-significantly influenced by drip irrigation methods and irrigation scheduling approaches.
Conclusions: Subsurface drip and ET sensor based irrigation triggering resulted in higher fruit and stalk yield which might be recommended for conserving irrigation water and reducing labour use. Whereas, the drip irrigation methods and irrigation scheduling approaches did not exert any significant influence on chemical properties of post-harvest soil.
Prevalence of Virulence Genes in Non-typhoidal Salmonella Isolates from Layers in Pantnagar, India
Authors: Nagpal Aastha , Maansi  , Upadhyay A.K. , Sharma Himani 
Published: July 2024

This study determined the prevalence of virulence genes in 23 non-typhoidal Salmonella (NTS) isolates obtained from layer breeds of an organized poultry farm in Pantnagar, India. Eight virulence genes viz. sipA, sopE1, fliC, mgtC, spvC, gipA, sopB, and stn were detected using the PCR technique. The most prevalent gene was sipA (95.65%), followed by sopB (73.91%), sopE1 (60.87%), stn (56.52%), fliC (47.83%), mgtC (30.43%), spvC (13.04%) and gipA (13.04%). Among all the isolates, one isolate didn’t show the presence of any virulence genes and none of the isolates harbored all the virulence genes screened. This study shows the high prevalence of virulence genes in NTS strains isolated from layers. Thus, continuous monitoring and surveillance of non-typhoidal Salmonella for virulence and its potential risks to humans and animals must be carried out.
Silicon Quantum Dots for Biological Applications
Authors: Chinnathambi Shanmugavel , Chen Song , Ganesan Singaravelu , Hanagata Nobutaka 
Published: August 2013

AbstractSemiconductor nanoparticles (or quantum dots, QDs) exhibit unique optical and electronic properties such as size‐controlled fluorescence, high quantum yields, and stability against photobleaching. These properties allow QDs to be used as optical labels for multiplexed imaging and in drug delivery detection systems. Luminescent silicon QDs and surface‐modified silicon QDs have also been developed as potential minimally toxic fluorescent probes for bioapplications. Silicon, a well‐known power electronic semiconductor material, is considered an extremely biocompatible material, in particular with respect to blood. This review article summarizes existing knowledge related to and recent research progress made in the methods for synthesizing silicon QDs, as well as their optical properties and surface‐modification processes. In addition, drug delivery systems and in vitro and in vivo imaging applications that use silicon QDs are also discussed.
Intestinal colonization: How key microbial players become established in this dynamic process
Authors: El Aidy Sahar , Van den Abbeele Pieter , Van de Wiele Tom , Louis Petra , Kleerebezem Michiel 
Published: August 2013

AbstractIn this review, we provide an overview of the dynamic changes within the microbiota and its metabolites that are implicated in establishing and maintaining gastrointestinal homeostasis during various stages of microbial colonization. The gradual conversion of the gut microbiota toward a mutualistic microbial community involves replacement of pioneer gut colonizers with bacterial taxa that are characteristic for the adult gut. An important microbial signature of homeostasis in the adult gut is the prevalence and activity of a diverse spectrum of bacterial species that produce beneficial metabolites through metabolic interactions between microbial groups. Deciphering these microbial signatures and their metabolites that govern short and long‐term equilibrium, as well as imbalances in host‐microbial relationships, may provide novel diagnostic tools and/or therapeutic targets for specific disorders associated with intestinal dysbiosis and loss of homeostasis.
Site‐targeted non‐viral gene delivery by direct DNA injection into the pancreatic parenchyma and subsequent in vivo electroporation in mice
Authors: Sato Masahiro , Inada Emi , Saitoh Issei , Ohtsuka Masato , Nakamura Shingo , Sakurai Takayuki , Watanabe Satoshi 
Published: August 2013

AbstractThe pancreas is considered an important gene therapy target because the organ is the site of several high burden diseases, including diabetes mellitus, cystic fibrosis, and pancreatic cancer. We aimed to develop an efficient in vivo gene delivery system using non‐viral DNA. Direct intra‐parenchymal injection of a solution containing circular plasmid pmaxGFP DNA was performed on adult anesthetized ICR female mice. The injection site was sandwiched with a pair of tweezer‐type electrode disks, and electroporated using a square‐pulse generator. Green fluorescent protein (GFP) expression within the injected pancreatic portion was observed one day after gene delivery. GFP expression reduced to baseline within a week of transfection. Application of voltages over 40 V resulted in tissue damage during electroporation. We demonstrate that electroporation is effective for safe and efficient transfection of pancreatic cells. This novel gene delivery method to the pancreatic parenchyma may find application in gene therapy strategies for pancreatic diseases and in investigation of specific gene function in situ.
A high‐throughput inhibition screening of major human cytochrome P450 enzymes using an in vitro cocktail and liquid chromatography–tandem mass spectrometry
Authors: Qin Chong‐Zhen , Ren Xian , Tan Zhi‐Rong , Chen Yao , Yin Ji‐Ye , Yu Jing , Qu Jian , Zhou Hong‐Hao , Liu Zhao‐Qian 
Published: August 2013

ABSTRACTA sensitive and high‐throughput inhibition screening liquid chromatography–mass spectrometry (LC‐MS/MS) method was developed and validated for the simultaneous quantification of five probe metabolites (7‐hydroxycoumarin, CYP2A6; 4‐hydroxytolbutamide, CYP2C9; 4′‐hydroxymephenytoin, CYP2C19; α‐hydroxymetoprolol, CYP2D6; and 1‐hydroxymidazolam, CYP3A4) for in vitro cytochrome P450 activity determination in human liver microsome and recombinant. All the metabolites and the internal standard, tramadol, were separated on a Waters 2695 series liquid chromatograph with a Phenomenex Luna C18 column (150 × 2.0 mm, 5 µm). Quality control samples and a positive control CYP inhibitor were included in the method. The IC50 values determined for typical CYP inhibitors were reproducible and in agreement with the literature. The method was selective and showed good accuracy (99.13–103.37%), and inter‐day (RSD < 6.20%) and intra‐day (RSD < 6.13%) precision. Also, the incubation extracts of the sample were stable at room temperature (20 °C) for 48 h and for 96 h in the autosampler (4 °C). The presented method is the first HPLC‐MS/MS method of this combination for simultaneous detection of the five metabolites 7‐hydroxycoumarin, 4‐hydroxytolbutamide, 4′‐hydroxymephenytoin, α‐hydroxymetoprolol and 1‐hydroxymidazolam in a single‐run process. It is possible that the high‐quality and ‐throughput cocktail provides suitable information in drug discovery and screening for new drug entities. Copyright © 2013 John Wiley & Sons, Ltd.
Mechanistic Insights into the Rhenium‐Catalyzed Alcohol‐To‐Olefin Dehydration Reaction
Authors: Korstanje Ties J. , Jastrzebski Johann T. B. H. , Klein Gebbink Robertus J. M. 
Published: August 2013

AbstractRhenium‐based complexes are powerful catalysts for the dehydration of various alcohols to the corresponding olefins. Here, we report on both experimental and theoretical (DFT) studies into the mechanism of the rhenium‐catalyzed dehydration of alcohols to olefins in general, and the methyltrioxorhenium‐catalyzed dehydration of 1‐phenylethanol to styrene in particular. The experimental and theoretical studies are in good agreement, both showing the involvement of several proton transfers, and of a carbenium ion intermediate in the catalytic cycle.
A Flexible Approach to Strained Sandwich Compounds: Chiral [1]Ferrocenophanes with Boron, Gallium, Silicon, and Tin in Bridging Positions
Authors: Sadeh Saeid , Schatte Gabriele , Müller Jens 
Published: August 2013

AbstractThe enantiomerically pure dibromoferrocene 3 [(Sp,Sp)‐1,1′‐dibromo‐2,2′‐di(isopropyl)ferrocene], equipped with two iPr groups in α positions, was prepared using known “Ugi amine” chemistry. Species 3 was targeted in order to gain access to new [1]ferrocenophanes ([1]FCPs) to be used as monomers for ring‐opening polymerization. The iPr groups on the sandwich unit were introduced to stabilize bridging moieties, as well as to increase solubilities of targeted metallopolymers. The planar chiral dibromide 3 can quantitatively be lithiated at 0 °C [2 equiv nBuLi, hexanes/thf (9:1), 30 min]. Salt‐metathesis reactions with respective element dichloride species gave chiral [1]FCPs with a variety of bridging moieties [ERx=Ga[2‐(Me2NCH2)C6H4] (4 a), SiMe2 (4 b), SntBu2 (4 c), BNiPr2 (4 d)]. The new [1]FCPs were fully characterized including single‐crystal X‐ray analysis. The stabilizing iPr groups on the Cp rings increase the thermal stabilities of 4 b–d compared to known [1]FCPs, equipped with the same bridging moieties. All three compounds 4 b–d are volatile and could be isolated by vacuum sublimation. Our new approach to [1]FCPs has the potential to overcome many of the existing difficulties in ferrocenophane chemistry, such as limited stability of starting monomers and low solubilities of resulting polyferrocenes.
Improved Chiral SFC Screening for Analytical Method Development
Authors: Schafer Wes , Chandrasekaran Tilak , Pirzada Zainab , Zhang Chaowei , Gong Xiaoyi , Biba Mirlinda , Regalado Erik L. , Welch Christopher J. 
Published: August 2013

ABSTRACTIn this study we describe the evaluation of a recently developed supercritical fluid chromatography (SFC) instrument for automated chiral SFC method development. The greatly improved gradient dwell volume and liquid flow control of the new instrument in combination with the use of shorter columns containing smaller stationary phase particles affords chiral SFC method development that is faster and more universal than previous systems. Chirality 25:799–804, 2013. © 2013 Wiley Periodicals, Inc.
Stereoselective Halocyclization of Alkenes With N‐Acyl Hemiaminal Nucleophiles
Authors: Liu Na , Wang Hao‐Yuan , Zhang Wei , Jia Zhi‐Hong , Guzei Ilia A. , Xu Hua‐dong , Tang Weiping 
Published: August 2013

ABSTRACTHalocyclization of alkenes was realized using N‐acylhemiaminal nucleophiles. High diastereoselectivity could be achieved for the formation of three stereogenic centers in this halogen‐mediated cyclization reaction. We also demonstrated that enantioselective bromocyclization of alkenes using N‐acylhemiaminal nucleophiles was possible. Chirality 25:805–809, 2013. © 2013 Wiley Periodicals, Inc.
Onset of Chiral Adenine Surface Growth
Authors: Capitán María Jose , Álvarez Jesús , Wang Yang , Otero Roberto , Alcamí Manuel , Martín Fernando , Miranda Rodolfo 
Published: August 2013

AbstractThe structure and stability of adenine crystals and thin layers has been studied by using scanning tunneling microscopy, X‐ray diffraction, and density functional theory calculations. We have found that adenine crystals can be grown in two phases that are energetically quasi‐degenerate, the structure of which can be described as a pile‐up of 2D adenine planes. In each plane, the structure can be described as an aggregation of adenine dimers. Under certain conditions, kinetic effects can favor the growth of the less stable phase. These results have been used to understand the growth of adenine thin films on gold under ultra‐high vacuum conditions. We have found that the grown phase corresponds to the α‐phase, which is composed of stacked prochiral planes. In this way, the adenine nanocrystals exhibit a surface that is enantiopure. These results could open new insight into the applications of adenine in biological, medical, and enantioselective or pharmaceutical fields.
Computational Study of the One‐ and Two‐Dimensional Infrared Spectra of a Proton‐Transfer Mode in a Hydrogen‐Bonded Complex Dissolved in a Polar Nanocluster
Authors: Sow Chia Shen , Tomkins Joseph , Hanna Gabriel 
Published: August 2013

AbstractThe signatures of nanosolvation on the one‐ and two‐dimensional (1D and 2D) IR spectra of a proton‐transfer mode in a hydrogen‐bonded complex dissolved in polar solvent molecule nanoclusters of varying size are elucidated by using mixed quantum–classical molecular dynamics simulations. For this particular system, increasing the number of solvent molecules successively from N=7 to N=9 initiates the transition of the system from a cluster state to a bulk‐like state. Both the 1D and 2D IR spectra reflect this transition through pronounced changes in their peak intensities and numbers, but the time‐resolved 2D IR spectra also manifest spectral features that uniquely identify the onset of the cluster‐to‐bulk transition. In particular, it is observed that in the 1D IR spectra, the relative intensities of the peaks change such that the number of peaks decreases from three to two as the size of the cluster increases from N=7 to N=9. In the 2D IR spectra, off‐diagonal peaks are observed in the N=7 and N=8 cases at zero waiting time, but not in the N=9 case. It is known that there are no off‐diagonal peaks in the 2D IR spectrum of the bulk version of this system at zero waiting time, so the disappearance of these peaks is a unique signature of the onset of bulk‐like behavior. Through an examination of the trajectories of various properties of the complex and solvent, it is possible to relate the emergence of these off‐diagonal peaks to an interplay between the vibrations of the complex and the solvent polarization dynamics.
Synthesis, characterization and hydrogen sorption studies of mixed sodium‐potassium alanate
Authors: Bhatnagar Ashish , Pandey Sunita K. , Shahi Rohit R. , Hudson M. Sterlin Leo , Shaz M. A. , Srivastava O. N. 
Published: August 2013

AbstractThe aim of the present investigation is to synthesize mixed sodium potassium alanate (K2NaAlH6) and to explore its hydrogen sorption characteristics. K2NaAlH6 is synthesized through ball milling of KH and NaAlH4 in the molar ratio 2:1 under hydrogen pressure of 10 bar. The temperature programmed desorption experiment shows that the synthesized K2NaAlH6 has peak desorption temperature of ∼352°C and reveals appreciable rehydrogenation kinetics under 6 bar hydrogen pressure at 300°C. The investigations are also focused on the catalytic effect of carbon nanostructures (CNS) namely, the graphene sheet (GS) and single wall carbon nanotube (SWCNT) and titanium halides (TiCl3 and TiF3) on K2NaAlH6. In the case of graphene and SWCNT catalyzed K2NaAlH6, the peak desorption temperature gets reduced to ∼347°C and ∼341°C respectively. The catalytic effects of CNS and titanium halide on K2NaAlH6 are also compared in the investigation. Between the two types of catalysts, halides are found to be better than CNS and out of the two halides, TiF3 is found to be the best catalyst for hydrogen sorption in K2NaAlH6. The peak desorption temperature decreases significantly from 352°C to ∼324°C for TiF3 catalyzed K2NaAlH6. Thus, the desorption activation energy reduces drastically from 124.43 kJ/mol (synthesized K2NaAlH6) to 88.05 kJ/mol for TiF3 catalyzed K2NaAlH6.