bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunotherapeutic potential of collagen V oral administration in mBSA/CFA-induced arthritis
Authors: Silveira Lizandre Keren Ramos da , Velosa Ana Paula , Catanozi Sergio , Pereira Marco Aurélio A. , Filho Antonio dos Santos , Marques Fabio Luiz N. , Faria Daniele de Paula , Real Caroline Cristiano , Fernezlian Sandra de M. , Yanke Amanda Flores , Queiroz Zelita Aparecida de J. , Contini Vitória Elias , Lobo Thays de Matos , Carrasco Solange , Baldavira Camila Machado , Goldenstein-Schainberg Cláudia , Fuller Ricardo , Capelozzi Vera L. , Teodoro Walcy R. 
Published: August 2024

Abstract

We hypothesized that after synovial injury, collagen V (Col V) expose occult antigens, and Col V autoantibodies develop, indicating the loss of immune tolerance against this molecule, thus leading to damage to mesenchymal-derived cells as well as the extracellular matrix in experimental arthritis. Thus, the present study investigated the effects of oral administration of Col V on the synovium after the development of inflammation in mBSA/CFA-induced arthritis. After fourteen days of intraarticular administration of mBSA, 10 male Lewis rats were orally administered Col V (500 µg/300 µL) diluted in 0.01 N acetic acid (IA-Col V group). The arthritic group (IA group, n = 10) received only intraarticular mBSA. An intra-articular saline injection (20 µL) was given to the control group (CT-Col V, n = 5). IA group presented damaged synovia, the expansion of the extracellular matrix by cellular infiltrate, which was characterized by T and B lymphocytes, and fibroblastic infiltration. In contrast, after Col V oral immunotherapy IA-Col V group showed a significant reduction in synovial inflammation and intense expression of IL-10 + and FoxP3 + cells, in addition to a reduction in Col V and an increase in Col I in the synovia compared to those in the IA group. Furthermore, an increase in IL-10 production was detected after IA-Col V group spleen cell stimulation with Col V in vitro. PET imaging did not differ between the groups. The evaluation of oral treatment with Col V, after mBSA/CFA-induced arthritis in rats, protects against inflammation and reduces synovial tissue damage, through modulation of the synovial matrix, showing an immunotherapeutic potential in inhibiting synovitis.
A challenging diagnosis of complete androgen insensitivity syndrome in a young Eritrean woman: a case report
Authors: mohammedidris sabrin , birara malede , tekeset simon 
Published: August 2024

Abstract

Background:Androgen insensitivity syndrome is a rare X-linked disorder in which a 46xy individual fails to virilize normally owing to varying degrees of impairment in androgen receptor function. Androgen insensitivity syndrome manifests in a spectrum of phenotypes.
Case presentation: A 27-year-old Eritrean who identified as a female presented with a history of primary amenorrhea and dyspareunia. Physical examination revealed the absence of pubic and axillary hair. The patient was tall, measuring 1.84 cm, and had a 4 cm long blind-ended vaginal pouch. Ultrasonography revealed an absent uterus and fallopian tubes. Hormone analysis and Barr body test were performed. The patient underwent orchidectomy.
Conclusion: Androgen insensitivity syndrome presents a broad spectrum of phenotypes. Therefore, a high index of suspicion is crucial among healthcare professionals to enable prompt diagnosis prior to the assignment of gender.
Evaluation of Demographic, Clinical, Laboratory Findings and Treatments of Our Febrile Seizures Cases
Authors: Yılmaz Özge Özdil , Karal Yasemin , Yılmaz Burak Barıs , Karaman Mustafa 
Published: August 2024

Abstract

Febrile convulsions are seizures during febrile illness between the ages of 6-60 months without a central nervous system infection or any other cause (electrolyte imbalance, metabolic disorder, intoxication, trauma) and without previous afebrile convulsions. It is the most common disorder of childhood neurological diseases.
In our study, we aimed to determine the characteristics among 200 patients who were diagnosed as febril convulsion at Trakya University Faculty of Medicine Department of Pediatrics, Pediatric Neurology Out-patient Clinic between January 2014-December 2018, to determine the risks of recurrence and development of epilepsy and patients’ response for different treatments.
Our patients’ male / female ratio is 1,22 / 1, 78% term, 60% NSVY and 20% of them have difficulty in labor. Diagnose age was between 6-60 months, with a mean of 21,82 ± 12,69 months. It was mostly seen under age of 2 (79%). Family history of febril seizure and epilepsy is %25 and 10 respectively. The common fever cause was upper respiratory system infections (83%). 41% was simple, 52,5% was complicated, and 82% was generalized tonic clonic. The recurrence rate was 22,5%. 8% of patients had abnormal EEG and they have higher recurrence rate. When the laboratory values were evaluated in terms of seizure recurrence and epilepsy, it was found that patients with recurrence have higher C-reaktive protein levels than those with epilepsy.
Patients with seizures below 38,3ºC, abnormal EEG and recurrence, have high rate of epilepsy.
Prophylaxis includes continuous antiepileptic, intermittant rectal diazepam and antipyretic. In our study recurrence after treatment in both seizure types is higher than those without treatment, but risk of developing epilepsy is not associated with treatment.
As a result; it is benign disease. Following-up is important because of the high recurrence rate and risk of epilepsy compared to general population. We found no significant difference between the treatments in preventing recurrence, but rectal diazepam may be preferred in those who received less recurrence, since it is easy to apply and has few side effects.
Comparative cross-sectional study of optimal screw positioning in the talus during arthroscopic ankle arthrodesis: A CT-based analysis
Authors: Kamijo Satoshi , Kumai Tsukasa , Tanaka Yasuhito 
Published: August 2024

Abstract

Objective: The prevalence of symptomatic ankle osteoarthritis increases with age; thus, affected individuals potentially have osteoporosis and talar fragility. Therefore, while performing osteosynthesis between the tibial plafond and the talar trochlea during arthroscopic ankle arthrodesis, increased stability and compression pressure during fixation are needed. Screw threads must be anchored within the talus; however, the bone mineral density of the talus has not been reported. We aimed to determine if the bone mineral density of the talus is lower in patients with ankle osteoarthritis than in healthy individuals, in addition to which part has the highest bone mineral density.
Materials and methods: We studied the talus of 10 feet without and 10 with end-stage ankle osteoarthritis. Each talar cancellous bone was divided into the lateral process, head and neck, middle body, and medial body. Computed tomographic of each segment was measured to calculate the relative bone mineral density difference between the regions.
Result: The mean computed tomographic values were 638.329 ± 139.765 standard deviation (SD), 465.960 ± 74.254 SD, 537.109 ± 82.443 SD, and 469.016 ± 84.490 SD of the healthy talus group and were 360.994 ± 117.403 SD, 284.397 ± 101.142 SD, 327.814 ± 114.772 SD, and 297.524 ± 105.667 SD in the end-stage ankle osteoarthritis talus group for the five respective segments above.
Conclusions: The bone mineral density values were significantly lower in the end-stage ankle osteoarthritis talus than in the healthy talus. The bone mineral relative density was highest from the body middle to the lateral process.
Performance evaluation of water supply service providers: a review with a focus on Brazil
Authors: Chaves Heloisa Pimpão , Branco Filho Thelmo de Carvalho Teixeira , Marques Rui Cunha , Malheiros Tadeu Fabrício 
Published: August 2024

ABSTRACT

This study aimed to analyze recent scientific literature concerning performance evaluation in the water supply sector, focusing on Brazil, and investigate factors that could influence its performance. For this purpose, we conducted a systematic literature review, following a series of steps to identify approaches, methodologies, and contexts into which the studies were developed. The search revealed that the studies focused on different management models and market structure issues. In addition, they also showed that the performance of service providers can be influenced by political and socioeconomic context and geographic aspects. A gap was identified concerning the qualitative studies since most used econometric methods mainly aimed at technical and financial efficiency. Also, social and service user perspectives received limited academic attention. To face the challenges in water provision in Brazil, tools, such as those related to performance evaluation, are essential for sufficient system management and can guide decision-makers and service providers.
Recent Molecular Targets and their Ligands for the Treatment of Alzheimer Disease
Authors: Bayraktar Gülşah , Alptüzün Vildan 
Published: August 2024

:
Alzheimer’s disease is a multifaceted neurodegenerative disease. Cholinergic dysfunction,
amyloid β toxicity, tauopathies, oxidative stress, neuroinflammation are among the main
pathologies of the disease. Ligands targeting more than one pathology, multi-target directed ligands,
attract attention in the recent years to tackle Alzheimer’s disease. In this review, we aimed
to cover different biochemical pathways, that are revealed in recent years for the pathology of the
disease, as druggable targets such as cannabinoid receptors, matrix metalloproteinases, histone
deacetylase and various kinases including, glycogen synthase kinase-3, mitogen-activated protein
kinase and c-Jun N-terminal kinase, and their ligands for the treatment of Alzheimer’s disease in
the hope of providing more realistic insights into the field.
Recent Development in Nanomedicine on the Use of Amygdalin in the Prevention and Treatment of Cancer: A Bibliometric Analysis
Authors: Prajapati Mahendra , Deshmukh Rohitas , K. Harwansh Ranjit 
Published: August 2024

Abstract:
Cancer is a serious health problem and one of the leading causes of death in humans in
both developing and underdeveloped countries. Researchers are using nanotechnology to improve
the specificity and effectiveness of cancer therapy as the number of cancer cases worldwide increases
daily. The aim of this bibliometric analysis is to examine current advances in
nanomedicine based on existing scientific work on the use of amygdalin in cancer research. Bibliometric
analysis was performed with VOSviewer software using the Scopus database. Out of 439
articles in the literature, 149 were found for bibliometric analysis, 11 of which were about
nanomedicine and were recently published. China was the most productive country in this field,
with Tabriz University being the leading research organization. The journals that published the
most articles on this topic were Anti-Cancer Agents in Medicinal Chemistry and Molecular Biology
Reports. The most frequently occurring keywords in these clusters were “cancer”,” “tumour”
and “amygdalin.” This work provides a summary of the literature with a graphical representation
of the research on the development of amygdalin through a bibliographic analysis. In addition,
this study offers a new perspective that could serve as a guide for future research directions.
Factors Influencing Dental Practitioners in the Management of Temporomandibular Joint Related Disorders in Clinical Practice – A Structural Equation Modelling Approach
Authors: Sivakumar Sasidharan , Sivakumar Gowardhan , Sundramoorthy Ashok K. 
Published: August 2024

Background
Temporomandibular joint disorders (TMJD) have shown a rising prevalence globally, affecting approximately 31% of adults/elderly and 11% of children/adolescents, with disc displacement with reduction (DDwR) being the most common condition. Despite the significant impact of TMJD on individuals and society, diagnosis and treatment remain inadequate. This study aims to identify factors that are hindering clinicians from managing TMJ-related disorders and to model these factors using latent variable structural equation modeling.

Methodology
A cross-sectional study was conducted from January to March 2023 among 470 dental practitioners in Chennai, Tamilnadu. The data were collected using a closed-ended questionnaire distributed via Google Forms, achieving an 87% response rate. Confirmatory factor analysis (CFA) and structural equation modeling (SEM) were used to analyze the data, focusing on clinician-related and patient-related factors.

Results
The measurement model was refined through CFA, achieving acceptable goodness-of-fit measures. The structural model revealed that clinician-related factors significantly impact the decision to manage TMJ disorders (β=0.96), with hesitation due to poor prognosis being the most influential factor. Patient-related factors, though significant, had a lesser impact (β=0.28), with economic affordability being the most influential patient-related factor.

Conclusion
The decision-making process for managing TMJ disorders is significantly influenced by clinician-related factors, with a lack of knowledge and hesitation due to poor prognosis being major barriers. Thus, enhancing the dental curriculum with comprehensive TMJ management training could improve clinician confidence and patient outcomes. Additionally, addressing patient affordability is crucial for effective treatment planning. This study highlights the need to address knowledge and competency gaps among general dental practitioners and provides insights to inform educational reforms, ultimately improving patient care outcomes.
The Effect of Various Water-soluble Chitosan Concentrations on Physical Properties and Antibiofilm Ability of Mineral Trioxide Aggregate
Authors: Maharti Ike Dwi , Ramadiani Nurul , Indrastuty Siringoringo Renny , Anri Zahra Khairiza , Meidyawati Ratna , Margono Anggraini , Kusumasari Citra 
Published: August 2024

Objective
Using Mineral Trioxide Aggregate (MTA) has several challenges as an apex closure material, such as a long hardening time, making it susceptible to dissolution and being washed away by blood flow, a gritty consistency that is difficult to manipulate, and low compression resistance. In addition, the antibacterial properties of MTA against E. faecalis, the bacteria persisting in periapical lesions, have shown controversial results. This study aimed to analyze the effect of adding various concentrations of water-soluble chitosan to MTA on the setting time, compression strength, and antibiofilm ability of E. faecalis.

Materials and Methods
There were three groups in this study, namely, MTA without water-soluble chitosan (MTA), MTA with 5% (MTA-CW5), and MTA with 10% (MTA-CW10) water-soluble chitosan. 0.5 g MTA powder (ProRoot MTA, Dentsply Tulsa Dental, Tulsa, OK, USA) was stirred in the MTA group with 0.166 ml of distilled water. In the MTA-CW5 and MTA-CW10 groups, 40 µl of 5% and 10% water-soluble chitosan was added to the mixture of 0.5 g MTA powder and 0.166 ml distilled water. Then, the three groups were tested for setting time, compression strength, and antibiofilm activity against E. faecalis. Setting time and compression strength values were analyzed by parametric statistics (ANOVA). Bacterial cell viability values on a numerical scale were statistically analyzed using the Kruskal–Wallis non-parametric analysis. The level of significance used was p < 0.05.

Results
There was a significant difference in setting time among the three groups, from the fastest to the slowest setting time, respectively: MTA-CW5, MTA, and MTA-CW10. The same thing happened in the compression strength test, with the lowest mean value shown in the MTA group at 50.53 + 6.18 MPa. The viability of E. faecalis between the MTA and MTA-CW5 groups did not have a statistically significant difference.

Conclusion
The setting time of MTA with 5% water-soluble chitosan was fast, but adding 10% water-soluble chitosan slowed the setting time of MTA. Meanwhile, increased water-soluble chitosan concentration led to increased compression strength and antibiofilm activity of MTA against E. faecalis.
Assessment of Chemical Composition and Investigation into the Antioxidant, Anti-inflammatory, and Hemolytic Properties of Hexane Extracts from Cynara cardunculus subsp. Cardunculus and Cynara cardunculus subsp. sylvestris
Authors: Meliani Nawel , Achiri Radja , Dib Mohammed El Amine , Muselli Alain 
Published: August 2024

Background:
Cynara cardunculus is frequently used in Mediterranean cuisine and is
known for its possible medicinal properties. These properties are usually related to the presence of
specific bioactive compounds present in the leaves of the artichoke. On the other hand, the root
parts of the artichoke have not been subjected to extensive studies so far.

Objective:
The main objective of this study was to conduct a chemical analysis of the root part of
the hexane extract of Cynara cardunculus subsp. cardunculus and Cynara cardunculus subsp.
sylvestris, while exploring their antioxidant, anti-inflammatory, and hemolytic effects.

Methods:
The chemical composition of the extracts of both species was analyzed using gas chromatography
(GC) and gas chromatography coupled with mass spectroscopy (GC/MS). The antioxidant
properties were evaluated using the DPPH radical scavenging method. The anti-inflammatory
activity was evaluated through the protein denaturation method using diclofenac as a positive
control. The hemolytic effect was examined on a suspension of erythrocytes in human blood.

Results:
The main constituents of the hexane extract of C. cardunculus and C. sylvestris were aplotaxene
(70.5% and 56.3%, respectively) and hexadecanoic acid (10.2% and 13.2%, respectively).
The hexane extracts of C. sylvestris and C. cardunculus showed positive antioxidant activity with
the DPPH test by comparing them with the BHT control. However, it should be noted that the extract
of C. cardunculus showed the best performance, with an IC50 of 4.3 μg/mL, while the extract
of C. sylvestris presented an IC50 of 5.6 μg/mL. The hexane extracts of C. cardunclus and C. sylvastris
showed good anti-inflammatory activity with IC50s of 17.3 μg/mL and 23.8 μg/mL compared
to diclofenac (IC50= 13.3 μg/mL), respectively. The toxicity assessment on human erythrocytes
shows that both extracts of roots of C. cardunculus and C. sylvestris have a very low hemolysis
rate (1.4% and 11.1%, respectively), even at high concentrations (2000 μg/mL).

Conclusion:
The extracts obtained from hexane from the plants C. cardunculus and C. sylvestris,
during the tests, revealed particularly promising antioxidant, anti-inflammatory, and hemolytic
properties. These results offer an interesting perspective for the creation and development of new
antioxidant and anti-inflammatory agents for the pharmaceutical and cosmetic industry.
Controlled Release of a Vasodilator Drug through Membrane-controlled Transdermal Systems: Development, Characterization, and Ex-vivo
Evaluation
Authors: Patil Sudha B. , Boppana Rashmi , Kulkarni Ravindra G. , Raghu Anjanpura V. , Kulkarni Raghavendra V. 
Published: August 2024

Background:
An attempt was made to develop and evaluate the membrane-
controlled transdermal systems for the controlled release of nicorandil. The
carbopol gel was selected as a drug reservoir, crosslinked blend membranes of XG
and SA were selected as rate-controlled membranes (RCM), and a film of poly(vinyl
alcohol) (PVA) was selected as the backing membrane.

Aim:
The aim of the current work was to formulate the membrane-controlled transdermal
drug delivery systems for the effective delivery of a vasodilator, nicorandil,
for the management of hypertension and angina pectoris, and in-vitro and ex-vivo
evaluation of these developed formulations.

Methods:
Reservoir gel was evaluated for drug content, pH, viscosity, and RCMs by
weight uniformity, thickness uniformity, differential scanning calorimetric (DSC)
analysis, x-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), water
vapor transmission, skin irritation, and histopathology.

Results:
Depending on crosslink density, variation in the water vapor permeation of
RCMs was noticed. The RCMs showed no signs of skin irritation. In-vitro drug permeation
through rat skin was extended for a period of 24 hours. With an increase in
acetaldehyde concentration in the RCM, there was a decrease in drug permeation.
Among the two terpenes used as penetration enhancers, the cineole at a concentration
of 20% exhibited a maximum permeation rate. The release mechanism from all systems
followed non-Fickian transport. Histopathology results indicated minor changes
in skin structure after skin permeation studies, which were reversible.

Conclusion:
The developed transdermal systems were found to have versatile dosage
forms for the controlled release of nicorandil, a vasodilator.
Analisis Kesulitan Guru dalam Mengajar di Masa Tatap Muka Terbatas Sekolah Dasar Kelas 2 Wilayah Desa Pacarejo
Authors: Wardani Sindi Asmiati 
Published: August 2024

Penelitian ini bertujuan untuk: (1) mendeskripsikan pelaksanaan pembelajaran tatap muka terbatas kelas 2 Sekolah Dasar di wilayah Desa Pacarejo; (2) mendeskripsikan dan memaparkan hambatan guru dalam pelaksanaan pembelajaran tatap muka terbatas kelas 2 Sekolah Dasar di Desa Pacarejo; dan (3) menganalisis dan mendeskripsikan upaya guru dalam mengatasi hambatan dalam pelaksanaan pembelajaran tatap muka terbatas kelas 2 Sekolah Dasar di Desa Pacarejo. Metode penelitian yang digunakan adalah penelitian deskriptif dengan pendekatan kuantitatif. Subjek penelitian yaitu sebanyak 8 orang yang menjadi guru kelas 2 Sekolah Dasar wilayah Pacarejo. Teknik pengumpulan data yang digunakan adalah angket. Analisis data yang digunakan adalah teknik analisis statistik deskriptif yang kemudian diinterprestasikan kedalam bentuk presentase. Hasil penelitian menunjukkan bahwa (1) Pelaksanaan pembelajaran tatap muka terbatas kelas 2 Sekolah Dasar di wilayah Desa Pacarejo mematuhi protokol kesehatan. Pembelajaran dilaksanakan secara terbatas yaitu 1 jam pelajaran selama 25 menit.; (2) Hambatan guru dalam pelaksanaan pembelajaran tatap muka terbatas dalam aspek prokes sebanyak 28%, materi sebanyak 42%, durasi waktu sebanyak 67%, interaksi sebanyak 91%, dan rombongan belajar sebanyak 69%; (3) Upaya guru dalam mengatasi kesulitan tersebut yaitu guru melakukan bimbingan dan pendekatan kepada siswa.
Implementasi Pelatihan Mandiri Terbimbing Penilaian Awal Literasi Membaca Bagi Guru Kelas Sekolah Dasar
Authors: Rohmah Nikmatur Nikmatur , Triyani Halifah Hastuti 
Published: August 2024

Platform Merdeka Mengajar (PMM) dirancang sebagai media pelatihan mandiri. Namun faktanya pemanfaatan PMM untuk meningkatkan pemahaman guru belum optimal. Penelitian ini bertujuan untuk mengembangkan dan mengevaluasi pelatihan mandiri terbimbing penilaian awal literasi membaca bagi guru kelas sekolah dasar. Desain penelitian deskriptif kualitatif. Tempat penelitian di SDN Wonorejo 03 pada September 2023 melibatkan 6 orang guru kelas dan 52 orang siswa. Teknik pengumpulan data menggunakan observasi, observasi partisipatif, wawancara terstruktur, dan studi dokumen. Teknik analisis data menggunakan deskriptif eksploratif dengan kerangka hermeneutika. Hasil studi ini menemukan pengembangan model pelatihan meliputi: identifikasi problem, investigasi konsep melalui PMM, membangung logika melalui bimbingan oleh Kepala Sekolah, project aksi nyata dan menemukan evidence metode early grade reading assessment (EGRA), interpretasi, refleksi dan umpan balik. Evaluasi hasil pelatihan menunjukkan bahwa guru lebih aktif menggunakan PMM dan dapat melakukan penilaian awal metode EGRA. Penelitian ini menyimpulkan bahwa dalam pelatihan mandiri terbimbing merupakan interaksi antara Guru, Kepala Sekolah, Platform Merdeka Mengajar, dan siswa dalam situasi belajar yang bermakna.