bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving Downstream Process Related Manufacturability Based on Protein Engineering—A Feasibility Study
Authors: Capito Florian , Wong Ting Hin , Faust Christine , Brand Kilian , Dittrich Werner , Sommerfeld Mark , Tiwari Garima , Langer Thomas 
Published: June 2024

ABSTRACTWhile bioactivity and a favorable safety profile for biotherapeutics is of utmost importance, manufacturability is also worth of consideration to ease the manufacturing process. Manufacturability in the scientific literature is mostly related to stability of formulated drug substances, with limited focus on downstream process‐related manufacturability, that is, how easily can a protein be purified. Process‐related impurities or biological impurities like viruses and host cell proteins (HCP) are present in the harvest which have mostly acid isoelectric points and need to be removed to ensure patient safety. Therefore, during molecule design, the surface charge of the target molecule should preferably differ sufficiently from the surface charge of the impurities to enable an efficient purification strategy. In this feasibility study, we evaluated the possibility of improving manufacturability by adapting the surface charge of the target protein. We generated several variants of a GLP1‐receptor‐agonist‐Fc‐domain‐FGF21‐fusion protein and demonstrated proof of concept exemplarily for an anion exchange chromatography step which then can be operated at high pH values with maximal product recovery allowing removal of HCP and viruses. Altering the surface charge distribution of biotherapeutic proteins can thus be useful allowing for an efficient manufacturing process for removing HCP and viruses, thereby reducing manufacturing costs.
Embracing Implementation Science to Enhance Fisheries and Aquatic Management and Conservation
Authors: Cooke Steven J. , Young Nathan , Alexander Steven , Kadykalo Andrew N. , Danylchuk Andy J. , Muir Andrew M. , Hinderer Julie L.M. , Cvitanovic Chris , Nguyen Vivian M. 
Published: June 2024

Abstract
The management and conservation of fisheries and aquatic resources are inherently applied activities. Therefore, when knowledge generated from research and monitoring, or knowledge that is held by practitioners and other actors (e.g., Indigenous elders, fishers), fails to inform those applied decisions, the persistent gap between knowledge and action is reinforced (i.e., the knowledge–action gap). In the healthcare realm, there has been immense growth in implementation science over the past decade or so with a goal of understanding and bridging the gap between knowledge and action and delivering on evidence-based decision making. Yet, within fisheries and aquatic sciences, the concept of implementation science has not received the same level of attention. We posit, therefore, that there is an urgent need to embrace implementation science to enhance fisheries and aquatic management and conservation. In this paper, we seek to describe what implementation science is and what it has to offer to the fisheries and aquatic science and management communities. For our context, we define implementation science as the scientific study of processes and approaches to promote the systematic uptake of research and monitoring findings and other evidence-based practices into routine practice and decision making to improve the effectiveness of fisheries management and aquatic conservation. We explore various frameworks for implementation science and consider them in the context of fisheries and aquatic science. Although there are barriers and challenges to putting implementation science into practice (e.g., lack of capacity for such work, lack of time to engage in reflection, lack of funding), there is also much in the way of opportunity and several examples of where such efforts are already underway. We conclude by highlighting the research needs related to implementation science in the fisheries and aquatic science realm that span methodological approaches, albeit a common theme is the need to involve practitioners (and other relevant actors) in the research. By introducing the concept and discipline of implementation science to the fisheries and aquatic science community, our hope is that we will inspire individuals and organizations to learn more about how implementation science can help deliver on the promise of evidence-based management and decision making and narrow the gap between research and practice.
Does college selectivity reduce obesity? A partial identification approach
Authors: Brunello Giorgio , Christelis Dimitris , Sanz‐de‐Galdeano Anna , Terskaya Anastasia 
Published: June 2024

AbstractWe use data from the National Longitudinal Study of Adolescent to Adult Health to investigate whether the quality of tertiary education ‐measured by college selectivity‐causally affects obesity prevalence in the medium run (by age 24–34) and in the longer run (about 10 years later). We use partial identification methods, which allow us, while relying on weak assumptions, to overcome the potential endogeneity of college selectivity as well as the potential violation of the stable unit treatment value assumption due to students interacting with each other, and to obtain informative identification regions for the average treatment effect of college selectivity on obesity. We find that attending a more selective college causally reduces obesity, both in the medium and in the longer run. We provide evidence that the mechanisms through which the impact of college selectivity on obesity operates include an increase in income, a reduction in physical inactivity and in the consumption of fast food and sweetened drinks.
Effects of atypical antipsychotics on serum asprosin level and other metabolic parameters in patients with schizophrenia
Authors: Amini Kiumarth , Motallebi Mohammad‐Javad , Bakhtiari Kimia , Hajmiri Minoo Sadat , Zamanirafe Maryam , Sharifikia Mahdis , Ranjbar Akram , Keshavarzi Amir , Mirjalili Mahtabalsadat , Mehrpooya Maryam 
Published: June 2024

AbstractBackgroundIn this cross‐sectional study, we compared fasting serum asprosin levels and metabolic parameters between patients receiving one of three atypical antipsychotics (olanzapine, risperidone, or aripiprazole) and healthy subjects.MethodsThe study population included 62 adult outpatients with schizophrenia and 22 healthy controls, matched for age and gender. Patients were in remission and had been on stable monotherapy with one of these atypical antipsychotics for over 6 months. Body Mass Index (BMI) and fasting serum levels of asprosin, glucose, HA1c, insulin, and lipid profile were compared across the investigated groups. Additionally, the number of participants meeting the insulin resistance criterion, defined as homeostasis model assessment for insulin resistance (HOMA‐IR) >2.5, as well as the number of participants with elevated BMI levels (men >27 kg/m2, women >25 kg/m2) were compared among the groups.ResultsWe observed statistically significant differences in BMI and fasting serum levels of glucose, HA1c, insulin, triglyceride (TG), high‐density lipoprotein cholesterol, and asprosin among patients receiving olanzapine or risperidone, as compared to those receiving aripiprazole and healthy subjects. Patients on aripiprazole exhibited values comparable to healthy subjects, whereas those on risperidone or olanzapine showed significantly higher values, with the highest observed in the olanzapine group. Additionally, the prevalence of participants meeting the insulin resistance criterion and those with elevated BMI was also greater in individuals receiving olanzapine or risperidone compared to those on aripiprazole and healthy subjects. Serum asprosin levels showed a significant positive correlation with BMI and several metabolic parameters, including HbA1c, fasting insulin, HOMA‐IR, and TG. No significant differences were observed among the investigated groups in terms of serum levels of total cholesterol and low‐density lipoprotein cholesterol.ConclusionsOur cross‐sectional study highlights the association between elevated asprosin levels, weight gain, and metabolic disorders in patients treated with olanzapine and risperidone. Given the bidirectional nature of the relationship between serum asprosin levels and these metabolic disturbances, further research is warranted to elucidate potential causative pathways.
Inundation dynamics in seasonally dry floodplain forests in southeastern Brazil
Authors: Meyer Oliveira Aline , van Meerveld Ilja , Gianasi Fernanda Moreira , Maciel Silva‐Sene André , Farrapo Camila Laís , de Carvalho Araújo Felipe , Oliveira Fernanda , Aparecido Silva Ferreira Leony , Carolina Arantes da Silva Lidiany , Gama Reis Miguel , Vieira Pompeu Patricia , Manoel dos Santos Rubens 
Published: June 2024

AbstractFloodplains are one of the most threatened ecosystems. Even though the vegetation composition in floodplain forests is expected to reflect the variation in groundwater levels and flood duration and frequency, there is little field data on the inundation dynamics (e.g., the variability in flood duration and flood frequency), especially for the understudied seasonally dry tropics. This limits our understanding of these ecosystems and the mechanisms that cause the flooding. We, therefore, investigated six floodplain forests in the state of Minas Gerais in Brazil for 1.5 years (two wet seasons): Capivari, Jacaré, and Aiuruoca in the Rio Grande basin, and Jequitaí, Verde Grande, and Carinhanha in the São Francisco basin. These locations span a range of climates (humid subtropical to seasonal tropical) and biomes (Atlantic forest to Caatinga). At each location, we continuously measured water levels in five geomorphologically distinct eco‐units: marginal levee, lower terrace, higher terrace, lower plain, and higher plain, providing a unique hydrological dataset for these understudied regions. The levees and terraces were flooded for longer periods than the plains. Inundation of the terraces lasted around 40 days per year. The levees in the Rio Grande basin were flooded for shorter durations. In the São Francisco basin, the flooding of the levees lasted longer and the water level regime of the levees was more similar to that of the terraces. In the Rio Grande basin, flooding was most likely caused by rising groundwater levels (i.e., “flow pulse”) and flood pulses that caused overbank flooding. In the São Francisco basin, inundation was most likely caused by overbank flooding (i.e., “flood pulse”). These findings highlight the large variation in inundation dynamics across floodplain forests and are relevant to predict the impacts of changes in the flood regime due to climate change and other anthropogenic changes on floodplain forest functioning.
Federal economic data on local and regional food producers
Authors: Bauman Allison , Jablonski Becca B. R. , Yeh D. Adeline , Chenarides Lauren , Thilmany McFadden Dawn 
Published: July 2024

AbstractUS local and regional food systems (LRFS) garner significant support from the federal government. Congress has directed the US Department of Agriculture to collect data on farmers and ranchers that use these markets, but challenges remain. We provide information about the three national surveys that provide farm‐level data on sales through LRFS, including how questions have changed over time. We highlight the benefits and challenges of using each survey from scale/scope and producer implication perspectives and offer recommendations to improve the existing survey instruments to support enhanced economic understanding of the implications of local and regional food markets.
14‐3‐3 Family of Proteins: Biological Implications, Molecular Interactions, and Potential Intervention in Cancer, Virus and Neurodegeneration Disorders
Authors: Low Zheng Yao , Yip Ashley Jia Wen , Chan Alvin Man Lung , Choo Wee Sim 
Published: July 2024

ABSTRACTThe 14‐3‐3 family of proteins are highly conserved acidic eukaryotic proteins (25–32 kDa) abundantly present in the body. Through numerous binding partners, the 14‐3‐3 is responsible for many essential cellular pathways, such as cell cycle regulation and gene transcription control. Hence, its dysregulation has been linked to the onset of critical illnesses such as cancers, neurodegenerative diseases and viral infections. Interestingly, explorative studies have revealed an inverse correlation of 14‐3‐3 protein in cancer and neurodegenerative diseases, and the direct manipulation of 14‐3‐3 by virus to enhance infection capacity has dramatically extended its significance. Of these, COVID‐19 has been linked to the 14‐3‐3 proteins by the interference of the SARS‐CoV‐2 nucleocapsid (N) protein during virion assembly. Given its predisposition towards multiple essential host signalling pathways, it is vital to understand the holistic interactions between the 14‐3‐3 protein to unravel its potential therapeutic unit in the future. As such, the general structure and properties of the 14‐3‐3 family of proteins, as well as their known biological functions and implications in cancer, neurodegeneration, and viruses, were covered in this review. Furthermore, the potential therapeutic target of 14‐3‐3 proteins in the associated diseases was discussed.
Loading thionine onto MXene enhances electron transfer and ultrasensitive electrochemical detection of H2O2
Authors: Dong Shaoqing , Sun Yue , Liu Teng , Wu Yongzheng , Song Wenxu , Zhou Qing 
Published: June 2024

AbstractAs an important reactive oxygen species (ROS) signal molecule in plant physiological regulation, H2O2 maintains cellular homeostasis through concentration regulation. It is worth paying attention to the concentration imbalance of H2O2 caused by various stresses, resulting in programed cell death or even developmental arrest in plants. To accurately quantify alterations in H2O2 concentration induced by these stress factors, and deeply understand the H2O2‐related physiological processes, a highly efficient hybrid electrode material of thionine@Ti3C2Tx (Th@MXene) composite was developed. MXene nanosheets not only performed as carriers with high specific surface area for loading Th but also contributed to the enhancement of electrical conductivity. Meanwhile, Th was uniformly loaded on the MXene surface, facilitating electron transport from the analyte to the modified electrode. Under the optimal detection conditions, the sensing electrode (Th@MXene/GCE) was employed to quantify H2O2 through Square‐wave Voltammetry signals with a good linear relationship (correlation coefficient is 0.9997), and a wide calibration range of the sensor was 0.1 to 10,000 nM. Above all, the detection limit can be as low as 34 pM, demonstrating excellent sensitivity. Additionally, the sensor exhibited repeatability in real samples, demonstrating exceptional practicality.
Arts and psychomotor therapies in personality disorder treatment: An appropriate therapeutic entrance to personal development: A commentary
Authors: Haeyen Suzanne , Dimaggio Giancarlo 
Published: June 2024

AbstractPersonality disorders (PD) are based not just on maladaptive ideas about self and others, they also are grounded on embodied patterns of behaviors and reactions to interpersonal stressors. There is growing interest in working with the body and through the body so to address automatisms that lead to suffering and dysfunctional social action. In this issue of the Journal of Clinical Psychology: In‐Session the use of art and psychomotor therapies for these patients was explored by seven different clinical perspectives. Patients described presented with different PD and associated symptoms. The arts and psychomotor therapies deployed in personality disorder treatment are: (visual) art therapy, music therapy, drama therapy, dance (movement) therapy, and psychomotor therapy making psychotherapeutic use of the different modalities: art, music, play, role‐play, performance, improvisation, dance, body awareness and movement. Interventions provide kinesthetic, sensory, perceptual, and symbolic opportunities to invite alternative modes of meaning‐making, accessing own needs and wishes, and communicating them to others. In this commentary we summarize some of the different topics covered by the clinical‐based papers, including working mechanisms of arts and psychomotor therapies, the importance of bottom‐up emotion regulation processes, how to treat trauma in the presence of a PD, how to integrate art and psychomotor therapies in a fine‐grained formulation and how to understand the process of change. Although there is a need for more empirical research, we hope this issue makes a solid case that clinicians can effectively include art and psychomotor therapies when treating the full range of PD.
IGF2 promotes the differentiation of chicken embryonic myoblast by regulating mitochondrial remodeling
Authors: Zhao Changbin , Hu Bowen , Zeng Xiaoyin , Zhang Ze , Luo Wen , Li Hongmei , Zhang Xiquan 
Published: July 2024

AbstractSkeletal muscle is crucial for animal movement and posture maintenance, and it serves as a significant source of meat in the livestock and poultry industry. The number of muscle fibers differentiated from myoblast in the embryonic stage is one of the factors determining the content of skeletal muscle. Insulin‐like growth factor 2 (IGF2), a well‐known growth‐promoting hormone, is crucial for embryonic and skeletal muscle growth and development. However, the specific molecular mechanism underlying its impact on chicken embryonic myoblast differentiation remains unclear. To elucidate the molecular mechanism by which IGF2 regulates chicken myoblast differentiation, we manipulated IGF2 expression in chicken embryonic myoblast. The results demonstrated that IGF2 was upregulated during chicken skeletal muscle development and myoblast differentiation. On the one hand, we found that IGF2 promotes mitochondrial biogenesis through the PGC1/NRF1/TFAM pathway, thereby enhancing mitochondrial membrane potential, oxidative phosphorylation, and ATP synthesis during myoblast differentiation. This process is mediated by the PI3K/AKT pathway. On the other hand, IGF2 regulates BNIP3‐mediated mitophagy, clearing dysfunctional mitochondria. Collectively, our findings confirmed that IGF2 cooperatively regulates mitochondrial biogenesis and mitophagy to remodel the mitochondrial network and enhance mitochondrial function, ultimately promoting myoblast differentiation.
Electromagnetic field‐induced adaptive response in Schwann cells through DNA methylation, histone deacetylation, and oxidative stress
Authors: Colciago Alessandra , Mohamed Tasnim , Colleoni Deborah , Melfi Valentina , Magnaghi Valerio 
Published: July 2024

AbstractSchwannomas are benign tumors of the peripheral nervous system arising from the transformation of Schwann cells (SCs). On the whole, these tumors are related to alterations of the neurofibromin type 2 gene, coding for the oncosuppressor merlin, a cytoskeleton‐associated protein belonging to the ezrin–radixin–moesin family. However, the underlying mechanisms of schwannoma onset and progression are not fully elucidated, whereas one of the challenges might be the environment. In this light, the exposure to electromagnetic field (EMF), generated by the use of common electrical devices, has been defiantly suggested as the cause of SCs transformation even if the evidence was mostly epidemiologic. Indeed, insubstantial mechanisms have been so far identified to explain SCs oncotransformation. Recently, some in vitro evidence pointed out alterations in proliferation and migration abilities in SCs exposed to EMF (0.1 T, 50 Hz, 10 min). Here, we used the same experimental paradigma to discuss the involvement of putative epigenetic mechanisms in SCs adaptation to EMF and to explain the occurrence of hypoxic alterations after the exposure. Our findings indicate a set of environmental‐induced changes in SCs, toward a less‐physiological state, which may be pathologically relevant for the SCs differentiation and the schwannoma development.
Social tipping and climate change: The moderating role of social capital in bridging the gap between awareness and action
Authors: Kaçani Krisdela , Kokthi Elena , López‐Bonilla Luis Miguel , González‐Limón Myriam 
Published: June 2024

AbstractBridging the gap between awareness and action on environmental issues such as climate change often requires understanding the moderating roles of individual trust, institutional trust and civic engagement. This paper explores how social capital, through generalaised trust, trust in institutions and civic engagement, can either strengthen or weaken the agency on climate change on environmental behaviour. Linking climate change awareness to environmental behaviour through the mediation of perceived changes in quality of life is the chosen approach to explore the role of social capital. The results suggest that low levels of trust, whether interpersonal or institutional, reduce an individual's sense of agency by firstly reducing the impact of climate change awareness on quality of life, changing perceptions and consequently reducing environmental behaviour. Greater trust in institutions produces a stronger effect of climate change awareness on willingness to pay. On the other hand, civic engagement shows a significant effect when taxes are considered. The study suggests that the impact of social capital on environmental payments varies according to the type of payment (voluntary vs. mandatory). The mapping of the role of social capital in reducing the agency of climate change awareness in quality‐of‐life changes should be further explored, as the latter has proven to be a promising way to address climate change in developed and developing countries.
Harvest of epithelialized gingival grafts without application of hemostatic sutures: A randomized clinical trial using laser speckle contrast imaging
Authors: Palombo David , Dobos Andrea , Duran Mercedes Lopez , Esporrin Javier Sanz , Sanz Mariano 
Published: June 2024

AbstractBackgroundThis randomized controlled trial with two parallel arms and a 1:1 allocation ratio aimed to compare early microvascular healing (primary outcome), surgical times, and patient‐reported outcomes (PROM) after harvesting palatal epithelialized gingival grafts (EGG), where hemostasis was achieved with sutures and hemostatic sponges (control) or with a sutureless approach (test).MethodsFrom a total of 33 patients, 34 EGG were harvested. Thirty‐two were randomized to the test/control group (n = 16) and two were excluded. Early palatal microvascular healing was assessed at 7, 14, and 30 days with laser speckle contrast imaging (LSCI). Postoperative bleeding, pain, discomfort, and analgesic consumption were assessed over 2 weeks with a dedicated questionnaire.ResultsA faster onset and resolution of postharvest hyperemia was observed in the test group where peak blood flow was reached at 7 days. No significant blood flow differences were observed between the groups at any of the evaluated timepoints. The mean surgical time was 13 min shorter in the test (p = 0.00). No significant differences were observed for postoperative bleeding and analgesic consumption at any timepoint.ConclusionsThe tested approach represents a viable alternative to the standard one, providing no relevant differences in microvascular, clinical, and patient‐related results, but with significantly shorter surgical times.