bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural and Dynamic Properties of Monoclonal Antibodies Immobilized on CNTs: A Computational Study
Authors: De Leo Federica , Sgrignani Jacopo , Bonifazi Davide , Magistrato Alessandra 
Published: July 2013

AbstractDue to the widespread application of carbon nanotube (CNT)‐based materials in nanomedicine, it is nowadays of paramount importance to unravel at the atomistic level of detail the structural properties of such bioconjugates in order to rationalize and predict the effect exerted by the graphitic framework on the bio‐active counterpart. In this paper, we report for the first time all‐atom explicit solvent molecular dynamics (MD) simulations investigating the structural and dynamic properties of a noncovalent bioconjugate in which the monoclonal Cetuximab antibody (Ctx) is adsorbed on a CNT surface. Upon selection of the three most representative adsorption modes as obtained by docking studies, force‐field MD and DFT simulations unambiguously showed that hydrophobic interactions mainly govern the adsorption of the protein on the graphitic surface. Two main adsorption poses have been predicted: a pose‐fab (p‐fab) and pose‐fc (p‐fc) (fab = fragment antigen binding region; fc = fragment crystallizable region), the former being favored with small‐diameter tubes (≤40 Å). In all the predicted poses, the secondary structure of Ctx is largely unaffected by the presence of the graphitic surface and, consistently with previous literature studies, our simulations reveal that positively charged amino acidic residues, such as Lys and Arg, predominantly contribute to the stabilization of the CNT⋅Ctx complex acting like surfactants. The predicted structural models are consistent with the experimental data, for which the immobilization of the antibody on CNTs does not disrupt the structural and recognition properties of the Ctx, consequently supporting the reliability of the used bioconjugation strategy for engineering stable and responsive hybrid nanomaterials for therapeutic applications. Moreover, a remarkable structural similarity of Ctx with antibodies of different isotypes suggests that in principle the CNT framework can interact in the same manner with all antibodies currently used in clinical applications.
Triage of women with minor abnormal cervical cytology: Meta‐analysis of the accuracy of an assay targeting messenger ribonucleic acid of 5 high‐risk human papillomavirus types
Authors: Verdoodt Freija , Szarewski Anne , Halfon Philippe , Cuschieri Kate , Arbyn Marc 
Published: July 2013

BACKGROUNDHigh‐risk human papillomavirus (hrHPV) DNA detection is generally accepted for the triage of women with a cytologic diagnosis of atypical squamous cells of undetermined significance (ASC‐US). However, no consensus has been reached on the optimal management of low‐grade squamous intraepithelial lesions (LSIL).METHODSIn this meta‐analysis, the diagnostic accuracy of nucleic acid sequence‐based amplification (NASBA) detection of messenger ribonucleic acid (mRNA) of 5 hrHPV types (the PreTect HPV‐Proofer and NucliSENS EasyQ tests) for detecting grade 2 cervical intraepithelial neoplasia or worse (CIN2+) and CIN3+ was assessed in women who had a diagnosis of ASC‐US and LSIL. The results were compared with the Hybrid Capture‐2 (HC2) assay, which detects the DNA of 13 hrHPV types. A bivariate random‐effect model that incorporated the intrinsic correlation between the true‐positive and false‐positive rates was used for a pooled meta‐analysis.RESULTSConsidering underlying CIN2+, the pooled absolute sensitivity of the 10 included studies was 75.4% (95% confidence interval [CI], 68.1%‐82.7%) and 76.2% (95% CI, 68.3%‐76.9%) for the triage of ASC‐US and LSIL, respectively. The pooled absolute specificity to exclude CIN2+ was 77.9% (95% CI, 70.1%‐85.7%) and 74.2% (95% CI, 69.5%‐78.8%) in women with ASC‐US and LSIL, respectively. Five studies allowed direct comparison of the mRNA assays with HC2. Considering CIN2+ in women with ASC‐US and LSIL, mRNA testing was substantially more specific than the HC2 assay (ratio: 1.98 and 3.36, respectively; P < .001) but was less sensitive (ratio: 0.80 and 0.74, respectively; P < .001).CONCLUSIONSHPV assays for detecting the mRNA of 5 hrHPV types may reduce the over‐diagnosis of women who have minor cytologic abnormalities. However, given the lower sensitivity, women with negative mRNA test results cannot be considered free of CIN2+ and require further surveillance. Cancer (Cancer Cytopathol) 2013;121:675–687. © 2013 American Cancer Society.
The frequency and cancer risk associated with the atypical cytologic diagnostic category in endoscopic ultrasound‐guided fine‐needle aspiration specimens of solid pancreatic lesions
Authors: Abdelgawwad Mohammad S. , Alston Evan , Eltoum Isam A. 
Published: July 2013

BACKGROUNDThe atypical cytologic diagnostic category is ambiguous and presents a management problem for pathologists and clinicians. This meta‐analysis reviewed the frequency and cancer risk associated with atypical diagnoses in endoscopic ultrasound‐guided fine‐needle aspiration (EUS‐FNA) specimens of solid pancreatic lesions.METHODSPubMed and Scopus were searched using the keywords “EUS‐FNA” and “pancreas.” Articles were screened focusing on studies of solid lesions. Studies with information regarding the frequency and outcomes of atypical diagnoses were included; the “suspicious” category was excluded from the analysis. The frequency of atypical diagnoses and the associated risk were calculated using the Comprehensive Meta‐Analysis software. The authors assessed whether the following factors explained the heterogeneity of the studies: rapid on‐site interpretation; type of reference standard; the study type, size, and site; and the frequency of inadequate, atypical, and positive categories.RESULTSA total of 23 studies with complete data regarding atypical diagnoses were identified, 12 of which had complete data available regarding outcomes. The frequency of the atypical category ranged from 1% to 14% (mean, 5.3%; 95% confidence interval, 4.1%‐6.9%). The risk of malignancy associated with an atypical diagnosis ranged from 25% to 100% (mean, 58%; 95% confidence interval, 47%‐69%). There was significant heterogeneity noted among the studies (I‐squared, 62%; P = .0004). The frequency of the atypical category and its associated risk were found to be correlated only with the frequency of the specimens being positive for malignancy.CONCLUSIONSThe rate of atypical diagnoses of the pancreas is similar to that of the thyroid but the risk of malignancy is higher. Significant heterogeneity exists among the studies reporting atypical diagnoses. There is a need for standardization of the reporting and management of atypical diagnoses in EUS‐FNA specimens from the pancreas. Cancer (Cancer Cytopathol) 2013;121:620–8. © 2013 American Cancer Society.
Nanodiamond–TiO2 Composites for Heterogeneous Photocatalysis
Authors: Pastrana‐Martínez Luisa M. , Morales‐Torres Sergio , Carabineiro Sónia A. C. , Buijnsters Josephus G. , Faria Joaquim L. , Figueiredo José L. , Silva Adrián M. T. 
Published: July 2013

AbstractInvited for this month’s cover are collaborators from the University of Porto in Portugal and KU Leuven in Belgium. The image shows that nanodiamond–TiO2 composites can effectively degrade organic water pollutants by photocatalysis under ambient conditions. This process will reduce human impact on freshwater systems, thereby contributing to meet worldwide targets on water footprint reduction. Read the full text of the article at 10.1002/cplu.201300094
Thermal Activation of Ammonia by Transition‐Metal Hydroxide Cations
Authors: Kretschmer Robert , Schlangen Maria , Schwarz Helmut 
Published: July 2013

AbstractInvited for this month’s cover is the group of Prof. Helmut Schwarz from Technische Universität Berlin, Germany. For this special issue dedicated to the memory of Detlef Schröder, the cover picture shows the then young Detlef—a mastermind dedicated to the sciences. Read the full text of the article on page 952.
Severely elevated transaminases in an adolescent male with anorexia nervosa
Authors: Smith Ryan W. , Korenblum Chana , Thacker Kunal , Bonifacio Herbert Joey , Gonska Tanja , Katzman Debra K. 
Published: July 2013

ABSTRACTAnorexia nervosa (AN) is a serious disorder that is associated with numerous medical complications and affects both females and males. Severely elevated transaminases have been reported in adult and younger females. We report the first case of elevated transaminases in an adolescent male with AN. The pathophysiologic mechanism of severely elevated serum transaminases observed in malnourished adolescent males with AN is complex and appears to be multifactorial. We present the first case of an adolescent male with AN who developed severely elevated serum transaminases that normalized with improved nutrition and weight gain. Liver injury in patients with AN is a complex medical complication that appears to be multifactorial in origin. In this case, starvation‐induced autophagy in the human liver was considered one of the most likely mechanisms to explain hepatocytic injury in this patient. © 2013 Wiley Periodicals, Inc. (Int J Eat Disord 2013; 46:751–754)
A General and Efficient Method To Convert Selenides into Selenones by Using HOF·CH3CN
Authors: Potash Shay , Rozen Shlomo 
Published: July 2013

AbstractA general route for the preparation of selenones (R2SeO2) is presented. This task is achieved through the quick and high‐yielding reaction of selenides (R2Se) with HOF·CH3CN. The reaction tolerates some elusive electron‐deficient and sterically hindered selenides. Some mechanistic aspects are also investigated and discussed.
Electrochemical Aptasensor Based on ZnO Modified Gold Electrode
Authors: Evtugyn Gennady , Cherkina Uljana , Porfireva Anna , Danzberger Jürgen , Ebner Andreas , Hianik Tibor 
Published: July 2013

AbstractWe developed an electrochemical thrombin aptasensor based on ZnO nanorods functionalized by electrostatically adsorption of 30‐mer DNA aptamers. The sensor surface was characterized by AFM and SEM. The surface layer assembling was optimized using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) with ferricyanide ions as redox markers. The peak current of the ferricyanide and the charge transfer resistance gradually decreased with increasing concentration of thrombin in the range from 3 pM to 100 nM due to formation of aptamer‐thrombin complexes and slower diffusion of the marker ions through the surface layer. At optimal conditions, a limit of detection (LOD) of 3 pM for EIS measurements and 10 pM for CV response was calculated from the S/N=3.
The Reporting of Fidelity Measures in Primary Prevention Programmes for Eating Disorders in Schools
Authors: Schober Ilka , Sharpe Helen , Schmidt Ulrike 
Published: July 2013

AbstractObjectiveThe aim of this paper was to examine the extent to which controlled trials of face‐to‐face school‐based primary prevention for eating disorders report their strategies for maintaining fidelity.MethodA systematic review located 38 articles eligible for inclusion. These studies were assessed using 18 criteria for reporting fidelity maintenance strategies based on those recommended by the Treatment Fidelity Workgroup of the National Institute of Health Behavior Change Consortium.ResultsFidelity reporting was generally poor. The studies reported between 22% and 56% of fidelity criteria. Detailed reporting of curriculum‐as‐usual control conditions was generally lacking, as were methods to ensure high‐quality training and mechanisms to assess provider adherence to intervention protocol.DiscussionPoor fidelity assessment and reporting is a problem in school‐based primary prevention programmes for eating disorders. Recommendations for improving fidelity maintenance and reporting practices are provided. Copyright © 2013 John Wiley & Sons, Ltd and Eating Disorders Association.
Heat transfer model for a cementitious‐based insulation with moisture
Authors: Sadiq H. , Wong M. B. , Al‐Mahaidi R. , Zhao X. L. 
Published: July 2013

SUMMARYThe lack of actual thermal properties of fire insulation materials lead to erroneous temperature prediction of protected structural steel members. For cementitious‐based fire insulations, moisture held in the pores significantly alters the thermal response of the material. In this study, a heat transfer model is proposed to accurately predict the thermal response of cementitious‐based fire insulations. The effects of the moisture and the decomposition of calcium hydroxide are incorporated in the thermal properties of the selected insulation material. The model was validated against the thermal response of the material obtained experimentally using the finite element programme abaqus (LAF Group, Sydney, NSW, Australia). Copyright © 2013 John Wiley & Sons, Ltd.
A Simple Route to New Cyclic (Chloroalkyl)phosphane‐, Diphosphane‐, and Aminophosphane Derivatives
Authors: Boga Carla , Micheletti Gabriele , Delpivo Camilla , Mazzacurati Marzia 
Published: July 2013

ABSTRACTThe reaction between benzothiadiphosphole and, in sequence, a bis‐Grignard and a chloroalkyl Grignard reagent has been reported. It produced, after treatment of the crude reaction mixture with elemental sulfur, new chloroalkyl cyclic tertiary phosphane sulfides. These compounds have been successfully employed as precursors of new nonsymmetric phosphoamino and diphosphano derivatives.
Histological effect on the adipocutaneous flap in rats after preconditioning with 2‐chloro‐N6‐cyclopentyladenosine
Authors: Dacho Andreas K. , Dietz Andreas , Mueller Kristin 
Published: July 2013

ABSTRACTBackground2‐chloro‐N6‐cyclopentyladenosine (CCPA) was proven to be a protective factor in ischemic reperfusion injury. The purpose of this study was to determine how CCPA would affect the single tissue layers of the adipocutaneous flap.MethodsSeventy male Wistar rats were divided into 5 experimental groups. Samples were taken of the area of flap necrosis and the wound margin after classical or pharmacological preconditioning on the fifth postoperative day. All samples were fixed in formaldehyde, embedded in paraplast, and analyzed in 3‐ to 4‐μm sections (hemalaun‐eosin stain and light microscopy).ResultsIn general, wound healing was alike and remained unaffected by the experimental design. The most sensitive part of the flap during preconditioning is the subcutis. The number of neutrophils and of plasma cells is reduced significantly (p < .05).ConclusionCCPA has an effect on each tissue layer of the flap. Subcutis became apparent as the most sensitive layer. CCPA influences complement pathway and neutrophils directly and indirectly. © 2014 Wiley Periodicals, Inc. Head Neck 36: 1189–1199, 2014
MDAI (5,6‐methylenedioxy‐2‐aminoindane; 6,7‐dihydro‐5H‐cyclopenta[f][1,3]benzodioxol‐6‐amine; ‘sparkle’; ‘mindy’) toxicity: a brief overview and update
Authors: Corkery John M , Elliott Simon , Schifano Fabrizio , Corazza Ornella , Ghodse A Hamid 
Published: July 2013

ObjectivesMDAI (5,6‐methylenedioxy‐2‐aminoindane; 6,7‐dihydro‐5H‐cyclopenta[f][1,3]benzodioxol‐6‐amine; ‘sparkle’; ‘mindy’) is a psychoactive substance, sold primarily over the Internet and in ‘head’ shops as a ‘legal high’. Synthesised and used as a research chemical in the 1990s, MDAI has structural similarities to MDMA (3,4‐methylenedioxy‐N‐methylamphetamine) and shares its behavioural properties. Recreational use of MDAI appears to have started in Europe around 2007, with a noticeable increase after 2009 in the UK and other countries. Calls to National Poisons Information Services started in 2010, although there were few presentations to emergency departments by patients complaining of undesirable physical and psychiatric effects after taking MDAI. Recreational use of this drug has been reported only occasionally by online user fora. There is little scientifically based literature on the pharmacological, physiological, psychopharmacological, toxicological and epidemiological characteristics of this drug.MethodsRecent literature (including ‘grey’) was searched to update what is known about MDAI, especially on its toxicity.ResultsThe resultant information is presented, including on the first three UK deaths involving MDAI use in 2011 and 2012. ‘Serotonin syndrome’ appears to be a possible factor in these fatalities.ConclusionIt is vital that any other cases, including non‐fatal overdoses, are documented so that a scientific evidence base can be established for them. Copyright © 2013 John Wiley & Sons, Ltd.
Consumer Debt Relief in South Africa—Should the Insolvency System Provide for NINA Debtors? Lessons from New Zealand
Authors: Coetzee Hermie , Roestoff Melanie 
Published: July 2013

AbstractSouth African natural person insolvency law has remained largely creditor‐orientated despite the international trend to assist over‐indebted debtors. Furthermore, although the South African system provides for a number of debt relief procedures, the entry requirements are of such a nature that most debtors are effectively excluded from any form of relief and therefore bound to their desperate situations. The majority of these excluded debtors fall within the no income and no assets (the so‐called No Income No Asset (NINA) debtors) category‐the main feature of this article. In the South African insolvency system, a person can therefore be ‘too poor to go bankrupt’. With reference to international principles and a thorough comparative study of the New Zealand system, the South African system is analysed, and some recommendations are made in order to provide a more accessible, effective and nondiscriminate system with specific focus on the plight of the NINA debtor. This is done by keeping the complex South African debt and poverty situation in mind as it is acknowledged that any reform should take cognisance of the unique socio‐economic and cultural background. It is recognised that providing relief to the NINA category debtors will have an impact on the economy. However, it is submitted that the exclusion of this group will be even more expensive as it creates an obstacle for these debtors to enter the formal sector and economy, thereby discouraging broader economic growth. Copyright © 2013 INSOL International and John Wiley & Sons, Ltd.
Detritus in small streams of the Tatra mountains – the role of abiotic factors
Authors: Galas Joanna 
Published: July 2013

AbstractThe quantity and quality of detrital input to alpine and forested mountain streams depends mainly upon the type of adjacent riparian vegetation: from alpine meadows to lower mountain forests. Benthic organic matter (BOM) and transported organic matter (TOM) content were quantified in six streams flowing in different vegetation zones of the Tatra Mountains. Annual BOM ranged from 0.1 g AFDM m−2 in the alpine meadow zone to 5.9 g AFDM m−2 in the lower mountain zone, and the coarse fraction of BOM was generally greatest. Similarly, average annual seston (particles <100 µm) concentrations increased from 0.1 to 0.4 mg AFDM l−1 in consecutive studied zones. VFPOM constituted 97% of total TOM. In the Tatra Mountains, stream BOM storage and the amount of TOM were negatively related to altitude and stream gradient and positively related to stream width and order. Very fine fractions of TOM in Tatra streams were negatively correlated with stream gradient. The performed analysis revealed some discrepancies concerning relation of BOM and TOM to stream gradient and order with that published for North American temperate zone streams from undisturbed forested catchments.
Aquatic hyphomycetes as survivors and/or first colonizers after a red sludge disaster in the Torna stream, Hungary
Authors: Vass Máté , Révay Ágnes , Kucserka Tamás , Hubai Katalin , Üveges Viktória , Kovács Kata , Padisák Judit 
Published: July 2013

AbstractA dam of a red sludge reservoir had ruptured on October 4, 2010 in Hungary and a mixture of 1 million m3 red sludge and water inundated two villages via the Torna stream. The industrial waste wiped out all detectable forms of life in the Torna stream, mainly due to its high pH value (pH 12.8). To study the fungal colonization leaves, Fagus sylvatica, Quercus cerris, Populus nigra, and Salix alba leaf litter were used as baits at two sites of the Torna stream. The leaves were sampled at seven occasions between February and June 2011 and a total of 26 aquatic hyphomycetes were revealed. Tricladium sp., Heliscus lugdunensis, and Tetracladium marchalianum were the dominant species at the impacted site. The total number of fungal species was lower at the impacted than at the reference site. The peak number of species associated with leaf litter was achieved later at the impacted site. Fungal species richness between leaf‐species at the two sites was compared by Fisher's exact test. Gypsum, which was dispersed into the stream in order to reduce the alkalinity, formed a layer on the leaves. Its effect for fungal colonization is discussed.
A Commentary on iPS Cells: Potential Applications in Autologous Transplantation, Study of Illnesses and Drug Screening
Authors: Romano Gaetano , Morales Fátima , Marino Ignazio R. , Giordano Antonio 
Published: July 2013

AbstractUndoubtedly, the focus of the field of stem cell research is predominantly aimed at the artificial reprogramming of human somatic cells for the production of induced pluripotent stem (iPS) cells. This relatively new technology may circumvent the ethical issues of using human embryonic stem (hES) cells for the potential applications in cell replacement therapy. Besides such ethical issues, iPS cell technology offers the advantage of obtaining patient‐derived tissues and/or cells, which may be utilized for autologous transplantation and tissue regeneration, investigation of a variety of human illnesses and for the screening of new drugs. The field of stem cell research has placed a major emphasis in understanding the genetic and epigenetic codes for pluripotency, in order to control and optimize autologous transplantation techniques and avoid teratoma formation. J. Cell. Physiol. 229: 148–152, 2014. © 2013 Wiley Periodicals, Inc.
PAP/HIP Protein Is an Obesogenic Factor
Authors: Secq Veronique , Mallmann Cecilia , Gironella Meritxell , Lopez Belen , Closa Daniel , Garcia Stéphane , Christa Laurence , Montalto Giuseppe , Dusetti Nelson , Iovanna Juan L. 
Published: July 2013

AbstractIn this article we report the obesogenic role of the acute phase protein PAP/HIP. We found that the transgenic TgPAP/HIP mice develop spontaneous obesity under standard nutritional conditions, with high levels of glucose, leptin, and LDL and low levels of triglycerides and HDL in blood. Accordingly, PAP/HIP‐deficient mice are skinny under standard nutritional conditions. We also found that expression of PAP/HIP is induced in intestinal epithelial cells in response to gavage with olive oil and this induction is AG490 sensitive. We demonstrated that incubation of 3T3‐L1 preadipocytes with a low concentration as 1 ng/ml of recombinant PAP/HIP results in accelerated BrdU incorporation in vitro. PAP/HIP‐dependent adipocytes growth is sensitive to the MEK inhibitor U0126. Finally, patients with severe obesity present higher blood levels of PAP/HIP than non‐obese control individuals. Altogether our data suggest that PAP/HIP could be a mediator of fat tissue development, released by the intestine and induced by the presence of food into the gut. J. Cell. Physiol. 229: 225–231, 2014. © 2013 Wiley Periodicals, Inc.