bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intracavernous Delivery of Freshly Isolated Stromal Vascular Fraction Rescues Erectile Function by Enhancing Endothelial Regeneration in the Streptozotocin-Induced Diabetic Mouse
Authors: Ryu Ji-Kan , Tumurbaatar Munkhbayar , Jin Hai-Rong , Kim Woo Jean , Kwon Mi-Hye , Piao Shuguang , Choi Min Ji , Yin Guo Nan , Song Kang-Moon , Kang Yong-Jin , Koh Young Jun , Koh Gou Young , Suh Jun-Kyu 
Published: October 2012

ABSTRACT

Introduction
Men with diabetic erectile dysfunction (ED) often have severe endothelial dysfunction and respond poorly to oral phosphodiesterase-5 inhibitors.

Aim
To examine whether and how freshly isolated stromal vascular fraction (SVF) promotes cavernous endothelial regeneration and restores erectile function in diabetic animals.

Methods
Eight-week-old C57BL/6J mice were used. Diabetes was induced by intraperitoneal injection of streptozotocin. SVF was isolated from epididymal adipose tissues of green fluorescence protein transgenic mice. At 8 weeks after the induction of diabetes, the animals were divided into six groups: controls, diabetic mice, and diabetic mice treated with a single intracavernous injection of phosphate-buffered saline (PBS) or SVF (1 × 104 cells, 1 × 105 cells, or 2 × 105 cells/20 µL, respectively).

Main Outcome Measures
Two weeks later, erectile function was measured by cavernous nerve stimulation. The penis was stained with antibodies to CD31, CD34, phosphohistone H3, phospho-endothelial nitric oxide synthase (eNOS), and vascular endothelial growth factor-A (VEGF-A). We also performed Western blot for phospho-eNOS and eNOS, and determined cyclic guanosine monophosphate (cGMP) concentration in the corpus cavernosum tissue.

Results
Significant improvement in erectile function was noted in diabetic mice treated with SVF at concentrations of 1 × 105 and 2 × 105 cells, which reached up to 82% of the control values. Local delivery of SVF significantly increased cavernous endothelial cell proliferation, eNOS phosphorylation, and cGMP expression compared with that in the untreated group and the PBS-treated diabetic group. Intracavernous injection of SVF increased cavernous VEGF-A expression and induced recruitment of CD34(+)CD31(−) progenitor cells. Some SVF underwent differentiation into cavernous endothelial cells. SVF-induced promotion of cavernous angiogenesis and erectile function was abolished in the presence of VEGF-Trap, a soluble VEGF-A neutralizing antibody.

Conclusion
The results support the concept of cavernous endothelial regeneration by use of SVF as a curative therapy for diabetic ED.
Testicular Augmentation Using Chin Implants
Authors: Ugarte y Romano Fernando , González Serrano Adolfo 
Published: October 2012

ABSTRACT

Introduction
Disturbances in testicular integrity have the potential to cause severe concerns about masculinity, body image, and sexual function. Testicular volume replacement surgery with prosthesis has been known about since 1941, although esthetic procedures for testicular augmentation have not been satisfactory.

Aim
To describe an unprecedented testicular augmentation surgical technique that is specially focused on preserving testicular function and providing a favorable esthetic outcome.

Methods
We present a case of a 45-year-old man with body dysmorphic disorder. Surgical treatment using a new technique by placing a chin implant on the testicle was offered and accepted.

Results
Good symmetry between both testicles was achieved. No complications were reported. One month after the procedure, the patient started a satisfactory sex life. Functional monitoring was normal during the year after the procedure.

Conclusion
This technique is unprecedented in literature, it represents a safe and effective therapeutic alternative; nevertheless, more experience in performing this procedure is needed.
Long-Term Changes of Sexual Function in Men with Obstructive Sleep Apnea After Initiation of Continuous Positive Airway Pressure
Authors: Budweiser Stephan , Luigart Ruth , Jörres Rudolf A. , Kollert Florian , Kleemann Yannick , Wieland Wolf F. , Pfeifer Michael , Arzt Michael 
Published: October 2012

ABSTRACT

Introduction
Obstructive sleep apnea (OSA), particularly intermittent nocturnal hypoxemia, is associated with erectile dysfunction (ED).

Aim
We investigated in patients with OSA whether continuous positive airway pressure (CPAP) therapy has a long-term effect on sexual function, including ED, in the presence of other risk factors for ED.

Methods
Within a long-term observational design, we reassessed 401 male patients who had been referred for polysomnography, with respect to erectile and overall sexual function. Mean ± standard deviation follow-up time was 36.5 ± 3.7 months. Patients with moderate to severe ED were stratified according to the regular use of CPAP.

Main Outcome Measure
Changes of sexual function were assessed by the 15-item International Index of Erectile Function (IIEF-15) questionnaire, including the domains erectile function (EF), intercourse satisfaction, orgasmic function (OF), sexual desire (SD), and overall satisfaction (OS).

Results
Of the 401 patients, 91 returned a valid IIEF-15 questionnaire at follow-up. Their baseline characteristics were not different from those of the total study group. OSA (apnea–hypopnea index &gt;5/hour) had been diagnosed in 91.2% of patients. In patients with moderate to severe ED (EF domain &lt;17), CPAP users (N = 21) experienced an improvement in overall sexual function (IIEF-15 summary score; P = 0.014) compared with CPAP non-users (N = 18), as well as in the subdomains OF (P = 0.012), SD (P = 0.007), and OS (P = 0.033). Similar results were obtained in patients with poor overall sexual dysfunction (IIEF-15 summary score &lt;44). In patients with moderate to severe ED and low mean nocturnal oxygen saturation (≤93%, median), also the EF subdomain improved in CPAP users vs. non-users (P = 0.047).

Conclusions
These data indicate that long-term CPAP treatment of OSA and the related intermittent hypoxia can improve or preserve sexual function in men with OSA and moderate to severe erectile or sexual dysfunction, suggesting a certain reversibility of OSA-induced sexual dysfunctions.
The Impact of Parental Bonding on Sexual Distress in Women with Type 1 Diabetes Mellitus
Authors: Bargiota Alexandra , Dimitropoulos Konstantinos , Mouzas Odysseas , Melekos Michael , Tzortzis Vassilios , Koukoulis Georgios 
Published: October 2012

ABSTRACT

Introduction
Psychosomatic and social issues have been found to be determinants of sexual distress in diabetic and non-diabetic populations. However, the role of parental bonding as a determinant for sexual distress has not been studied in women with type 1 diabetes mellitus (DM-1).

Aim
To study the role of parental care and overprotection, in the pathogenesis of sexual distress in women with DM-1.

Methods
Seventy-seven women with uncomplicated DM-1 and 77 healthy controls were enrolled in the study. The Female Sexual Distress Scale (FSDS), the General Health Questionnaire-28, and the Parental Bonding Instrument were used to evaluate sexual distress, general health and bonding with parents, respectively.

Main Outcome Measures
To assess the role of parental bonding as risk factor for sexual distress, in women with DM-1.

Results
Women with DM-1 had significantly higher FSDS scores compared with controls. Furthermore, women with DM-1 had significantly higher maternal and paternal care, and lower maternal overprotection in comparison with the healthy ones. Paternal overprotection and general health were similar in both groups (P &gt; 0.05). Sexual distress was more frequent in women with DM-1 (31.43% vs. 8.57% of controls, P &lt; 0.05).
Diabetic women with sexual distress had lower maternal care, higher maternal overprotection and lower paternal overprotection compared to diabetics without sexual distress (P &lt; 0.05). No difference was found in the paternal care between the two groups (P &gt; 0.05). Moreover, sexually distressed DM-1 women had worse general health parameters in comparison with the non-sexually distressed diabetics (P &lt; 0.05). In the DM-1 group, low maternal care and low paternal overprotection were significant risk factors for sexual distress (P &lt; 0.05).

Conclusions
Parental care and overprotection can lead to sexual distress and, therefore, to Female Sexual Dysfunction in DM-1 women. Evaluation of parental bonding is necessary in DM-1 women with distressing sexual problems.
The Global Online Sexuality Survey (GOSS): The United States of America in 2011 Chapter II: Phosphodiesterase Inhibitors Utilization Among English Speakers
Authors: Shaeer Osama 
Published: October 2012

ABSTRACT

Introduction
Utility of phosphodiesterase inhibitors (PDEi's) for the treatment of erectile dysfunction (ED) has been the focus of experimental and clinical studies. However, public preferences, attitudes, and experiences with PDEi's are rarely addressed from a population/epidemiology viewpoint. The Global Online Sexuality Survey (GOSS) is a worldwide epidemiologic study of sexuality and sexual disorders, first launched in the Middle East in 2010, followed by the United States in 2011.

Aim
To describe the utilization rates, trends, and attitudes toward PDEi's in the United States in the year 2011.

Methods
GOSS was randomly deployed to English-speaking male Web surfers in the United States via paid advertising on Facebook®, comprising 146 questions.

Main Outcome Measures
Utilization rates and preferences for PDEi's by brand.

Results
Six hundred three subjects participated; mean age 53.43 years ± 13.9. Twenty-three point seven percent used PDEi's on more consistent basis, 37.5% of those with ED vs. 15.6% of those without ED (recreational users). Unrealistic safety concerns including habituation were pronounced. Seventy-nine point six percent of utilization was on prescription basis. PDEi's were purchased through pharmacies (5.3% without prescription) and in 16.5% over the Internet (68% without prescription). Nine point six percent nonprescription users suffered coronary heart disease. Prescription use was inclined toward sildenafil, generally, and particularly in severe cases, and shifted toward tadalafil in moderate ED and for recreational use, followed by vardenafil. Nonprescription utilization trends were similar, except in recreational use where sildenafil came first.

Conclusion
In the United States unrealistic safety concerns over PDEi's utility exist and should be addressed. Preference for particular PDEi's over the others is primarily dictated by health-care providers, despite lack of guidelines that govern physician choice. Online and over-the-counter sales of PDEi's are common, and can expose a subset of users to health risks. Recreational use of PDEi's is common, and could be driven by undiagnosed premature ejaculation.
Factors Influencing Distress Toward Erectile Dysfunction and Attitude Toward Erectile Dysfunction Drugs Among Middle-Aged and Elderly Chinese Women and Men in Hong Kong
Authors: Liong Chan Ching Mario 
Published: October 2012

ABSTRACT

Aim
This study aims to explain distress toward erectile dysfunction (ED), attitude toward ED drugs, and experiences with ED drug use in terms of sexual attitudes, relationship satisfaction, and sociodemographic factors among the middle-aged and elderly Chinese population. Studies show that a significant number of middle-aged and elderly men in Asia suffer from ED. However, people's attitudes toward ED drugs are not positive. Few studies have sought to reveal the influencing factors of this negative attitude.

Methods
Nine hundred forty-six Hong Kong women and men aged 36–80 with stable partners were administered a structured questionnaire through face-to-face street-intercept survey. Self-reporting on single-item questions using a five-point Likert scale was adopted for most of the variables, including attitude toward sex, relationship satisfaction, distress toward ED, and attitude toward ED drugs. Perceived importance of sex in an intimate relationship and attitude toward sex were assessed through three items. Respondents were asked to report whether they had taken ED drugs or bought ED drugs for their partners.

Results
While perceived importance of sex in an intimate relationship and distress toward ED both contributed to the estimation of attitudes toward ED drugs for both genders, relationship satisfaction was only associated with men's attitude, while women's attitude was related to two other factors: relationship status and income level. Both age and attitude toward ED drugs influenced experiences with ED drug use for women and men. Men's experience with ED drug use was further related to the perceived importance of sex and to men's self-esteem and relationship status.

Conclusion
Results of this study could help identify people who are disturbed by ED but reluctant to seek treatment. Clinicians and public health policy makers could make use of the findings to encourage proper understanding of ED.
Radical Cystectomy in Patients with Preexisting Three-Piece Inflatable Penile Prosthesis
Authors: Segal Robert L. , Readal Nathaniel , Pieororazio Philip M. , Kutlu Omer , Schoenberg Mark , Bivalacqua Trinity J. 
Published: October 2012

ABSTRACT

Introduction
Radical cystectomy with urinary diversion is the treatment of choice for muscle-invasive, and certain populations with non-invasive, urothelial carcinoma of the bladder. There have not been any reports to date on patients undergoing this surgery who have had previous placement of an inflatable penile prosthesis.

Aim
To present the outcomes of four patients with pre-existing inflatable penile prostheses (IPP) with reservoirs within the space of Retzius who were subsequently treated with radical cystectomy for bladder cancer management.

Methods
After obtaining institutional review board approval, the demographic, clinical, and pathologic data were reviewed in the Johns Hopkins Cystectomy Database for patients who underwent radical cystectomy for bladder cancer from 1994 to 2012. A case series of four patients is presented who had a preexisting IPP and their post-operative course and long-term outcomes are reviewed.

Results
All four patients had radical cystectomy and ileal conduit urinary diversion with no intra-operative or post-operative complications. One patient was not sexually active and therefore had the reservoir explanted and not replaced. The other three patients had the reservoir removed prior to bladder extirpation and the tubing capped, with reservoir replacement in the pseudocapsule at the termination of the procedure. In one patient an omental flap was used to ensure separation between the reservoir and ileal conduit. The devices were all functional intra-operatively and on follow-up.

Conclusions
As erectile dysfunction is more commonly being diagnosed and treated with IPP insertion at younger ages, surgeons will increasingly encounter pre-placed abdominal reservoirs when performing pelvic surgery. This case series of four patients undergoing radical cystectomy with prior-placed IPPs reveals that the functionality of the IPP can be preserved while still performing oncologically sound extirpative procedures.
Evaluation of pulsed electromagnetic field therapy in the management of patients with discogenic lumbar radiculopathy
Authors: Omar Aziza Sayed , Awadalla Magdy Ahmed , El‐Latif Maii Abd 
Published: October 2012

AbstractAimThis randomized clinical trial was designed to evaluate the effect of pulsed electromagnetic field therapy (PEMF) in the management of patients with discogenic lumbar radiculopathy.MethodsForty patients suffering from lumbar radiculopathy due to lumbar disc prolapse were randomly assigned to one of two groups: a study group that included 20 patients who received PEMF therapy and a control group that included 20 patients who received placebo treatment. Both groups were evaluated at bases line and after 3 weeks by using a visual analogue scale (VAS) (0–10), somatosensory evoked potentials (SSEPs) for selected dermatomes and Modified Oswestry Low Back Pain Disability Questionnaire (OSW), and findings were compared before and after treatment.ResultsSignificant differences were observed between both groups before and after application of PEMF therapy relative to VAS (P = 0.024), total OSW (P < 0.001), and other domains of OSW score (pain intensity [P = 0.009], personal care [P = 0.01], lifting [P < 0.001], walking [P < 0.001], sitting [P < 0.001], standing [P < 0.001], sleeping [P < 0.001], social life [P < 0.001] and employment [P = 0.003]). Other significant differences were observed between both groups relative to SSEP latency and amplitude of the evaluated dermatomes on the right side (P = 0.022 and P = 0.001, respectively), and left side latency and amplitude (P = 0.016 and P = 0.002, respectively).ConclusionPEMF therapy is an effective method for the conservative treatment of lumbar radiculopathy caused by lumbar disc prolapse. In addition to improvement of clinically observed radicular symptoms, PEMF also seems effective in reducing nerve root compression as evidenced by improvement of SSEP parameters after treatment.
Effects of Exogenous Abscisic Acid on Antioxidant System in Weedy and Cultivated Rice with Different Chilling Sensitivity under Chilling Stress
Authors: Wang G.‐J. , Miao W. , Wang J.‐Y. , Ma D.‐R. , Li J.‐Q. , Chen W.‐F. 
Published: October 2012

AbstractTwo chilling‐tolerant genotypes, that is, weedy rice WR03‐45 and cultivated rice Lijiangxintuanheigu and two chilling‐sensitive genotypes, that is, weedy rice WR03‐26 and cultivated rice Xiuzinuo were used in this study to investigate the effects of exogenous abscisic acid (ABA) on protection against chilling damage as well as on changes in physiological features. The results showed that under chilling stress the increased levels of superoxide radical (), hydrogen peroxide (H2O2) and malondialdehyde (MDA) in WR03‐45 and Lijiangxintuanheigu were lower than those in WR03‐26 and Xiuzinuo. Activities of antioxidant enzymes (superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR)) and non‐enzymatic antioxidants (ascorbate acid (AsA) and reduced glutathione (GSH)) were enhanced in WR03‐45 and Lijiangxintuanheigu, whereas they were decreased significantly in WR03‐26 and Xiuzinuo. Application of exogenous ABA reduced the chilling damage in the four genotypes. The pre‐treatment with ABA decreased the levels of , H2O2 and MDA caused by chilling stress in the four genotypes through increasing the activities of SOD, CAT, APX, GR and the contents of AsA and GSH in the four genotypes under chilling stress. Moreover, pre‐treatment with Fluridone, the ABA biosynthesis inhibitor, prohibited the effects of ABA through enhancing the oxidative damages and suppressing the antioxidant defence systems under chilling stress. The results indicate the mechanism for rice with chilling tolerance is to enhance the capacity of antioxidant defence systems under chilling stress. Furthermore, ABA plays important roles in the tolerance of rice against chilling stress for it could induce the capacity of whole antioxidant defence systems including enzymatic and non‐enzymatic constitutions under chilling stress.
Role of Defibrillation Threshold Testing in the Contemporary Defibrillator Patient Population
Authors: VISCHER ANNINA S. , STICHERLING CHRISTIAN , KÜHNE MICHAEL S. , OSSWALD STEFAN , SCHAER BEAT A. 
Published: October 2012

Role of Defibrillation Threshold Testing.
Introduction: Defibrillation threshold (DFT) testing has been performed to prove functionality of the implantable cardioverter defibrillator (ICD). Over the past years it has become increasingly controversial because of possible morbidity and mortality. The goal of this study was to determine unsuccessful shock testing and report strategies used to overcome these problems.Methods and Results: A total of 314 patients with a de novo implantation of an ICD and 127 patients receiving a generator exchange were identified retrospectively. All patients underwent defibrillation threshold testing after induction of VF using a low‐energy T‐wave shock during the intervention, 2 shock tests after de novo implantations, 1 after generator change. A safety margin of 10 J or more was requested. Seven (2.3%) patients in the de novo group and 2 patients (1.4%) in the generator exchange group could not be defibrillated using the standard approach. All of those patients had either chronic amiodarone therapy, secondary prevention or a cardiac resynchronization therapy device (CRT). In univariate analysis, amiodarone therapy, dilated cardiomyopathy, and lower ejection fraction were predictors of failure.Conclusion: Our study's results as well as a review of the current literature favor shock testing, especially in patients with specific risk factors as mentioned above. (J Cardiovasc Electrophysiol, Vol. 24, pp. 437‐441, April 2013)
Impact of the Number of Blood Pressure Measurements on Blood Pressure Classification in US Adults: NHANES 1999–2008
Authors: Handler Joel , Zhao Yumin , Egan Brent M. 
Published: October 2012

J Clin Hypertens (Greenwich). 2012;14:751–759. ©2012 Wiley Periodicals, Inc.Clinical guidelines recommend averaging ≥2 blood pressure (BP) measurements on each visit. Only one BP is measured on many clinical visits, especially if the value is <120/<80 mm Hg, ie, normal. The impact of this practice on accurate assignment of BP category is incompletely defined. Data were analyzed from 22,641 adults 18 years and older who had 3 BP readings in the National Health and Nutrition Examination Surveys 1999–2008. BP category defined by initial measurement was compared with the category determined by mean of the first and second, first through third, and second and third readings. Among 8553 nonhypertensive patients with initial BP <120/<80 mm Hg, 2.9%, 3.3%, and 6.7%, respectively, were reclassified as prehypertensive, ie, BP 120–139/80–89 mm Hg, and two patients as stage 1 hypertension (140–159/90–99 mm Hg). In 733 treated hypertensive patients with initial BP <120/<80 mm Hg, 5.1%–8.9% were reclassified as prehypertensive and only one patient as hypertensive. Among nonhypertensive and hypertensive patients with initial BP in the prehypertensive range, 8.0%–23.6% were reclassified as normal. Among stage 1 and 2 hypertensive patients based on initial BP, 18.2%–33.5% were reclassified to lower BP categories. By multivariable logistic regression, older age and higher systolic and diastolic BP were associated with reclassification to a lower BP category. In nonhypertensive and hypertensive patients with normal initial BP values, one BP measurement appears adequate as <10% are re‐classified as prehypertensive and <0.5% as hypertensive. In contrast, patients with an initial BP above normal are often reclassified to a lower category, which supports recommendations for additional measurements.