bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sistema Único de Saúde: Desafios, Conquistas e Caminhos para o Futuro
Authors: Rodrigues Laura de Nazaré Mendes , Souza Marina Cláudia Aguiar de , Antunes Erika Cristina Brasil , Neves Larissa Malcher das , Rodrigues Thiago Matheus Barbosa , Silva Thayane e Silva , Filgueira Glayce Maria Carneiro , Diniz Josimara Ribeiro , Costa Tayse Alves da , Braga Jaqueline de Aguiar , Salame Youssef Camil Yazbek , Ferreira Tayná Vitória Silva Santos , Barros Ana Karolina do Amor Divino , Macedo Mirla da Silva , Cunha Layse Pereira da , Magno Gabriel Risuenho , Moraes Keyse Karina Martins , Oliveira Hugo Coimbra de , Silva Bianka da Paixão , Santos Gercila Tiburcia da Silva 
Published: February 2025

Este livro oferece uma análise abrangente e reflexiva sobre o Sistema Único de Saúde (SUS), um dos maiores e mais complexos sistemas de saúde pública do mundo. Com uma abordagem interdisciplinar, a obra explora os desafios históricos e atuais enfrentados pelo SUS, as conquistas que consolidaram sua relevância para a sociedade brasileira e os caminhos possíveis para seu fortalecimento no futuro.
Dividido em capítulos que abrangem aspectos políticos, econômicos, sociais e tecnológicos, o livro aborda temas como:
•	A origem e os princípios do SUS: universalidade, integralidade e equidade como pilares fundamentais;
•	Gestão e financiamento: desafios para garantir sustentabilidade e eficiência;
•	Inovações e tecnologias em saúde: o impacto da digitalização e da inteligência artificial na gestão e nos serviços;
•	Determinantes sociais da saúde: como desigualdades sociais afetam o acesso e a qualidade do cuidado;
•	Participação social e controle democrático: a importância da cidadania ativa no fortalecimento do SUS;
•	A pandemia de COVID-19: lições aprendidas e o papel do SUS na resposta a crises sanitárias.

Reunindo contribuições de especialistas, gestores, acadêmicos e profissionais da saúde, a obra apresenta perspectivas diversificadas sobre os caminhos para aprimorar o SUS, promovendo um sistema mais resiliente, inclusivo e eficaz.
Sistema Único de Saúde: Desafios, Conquistas e Caminhos para o Futuro é uma leitura indispensável para estudantes, pesquisadores, profissionais e gestores que buscam compreender e contribuir para o desenvolvimento sustentável do SUS, reafirmando seu papel central na promoção da saúde e da equidade social no Brasil.
PENGEMBANGAN INSTRUMEN TES BERBASIS ANDROID PADA MATERI RUMUS-RUMUS TRIGONOMETERI KELAS X
Authors: Sundari Elvira , Izzati Nur 
Published: February 2025

The government expects practicality in using the budget, especially in conducting testing. With the development of technology in the revolutionary era 4.0, the government was able to reduce the budget for conducting tests, namely by using technology. This study aims to develop an android-based test instrument on the appropriate material for trigonometric formulas for class XI SMA. This type of research is Research and Development by applying the Borg and Gall model. This study involved 32 students of class XI MIPA 4 at SMAN 2 Tanjungpinang. The data analysis technique used was content validity and item analysis. The results obtained were 98.9% categorized as very valid by material experts and 100% categorized as very valid by mDevelopmentedia experts and the results of the item analysis showed valid and reliable, the level of difficulty of the test instrument was 68% categorized as moderate, and the distinguishing power of the test instrument was 59% categorized as good.
Implementation of the quality-by-design concept for an adeno-associated viral vector-based gene therapy
Authors: Kopein D. S. , Poroshin G. N. , Khamitov R. A. 
Published: February 2025

INTRODUCTION. Currently, manufacturers of adeno-associated virus (AAV)-based gene therapy products are facing a number of systemic problems stemming from the difficulties in assessing the quality of medicinal products due to insufficient scientific data, limited experience, and imperfect regulatory requirements. However, a risk-based approach to assessing critical quality attributes (CQAs) within the the framework of Quality by Design (QbD) can ensure improved efficiency in the development and production of advanced therapy medicinal products.AIM. This study aimed to identify QbD-based CQAs and associated specifications for the development of AAV-based gene therapy products for Duchenne muscular dystrophy.DISCUSSION. This study involved an analysis of QbD-based approaches to the development of AAV production technologies. The authors substantiated a list of the main AAV characteristics and collated available data on their impact on patients in terms of the efficacy and safety of gene therapy products and, in particular, the immune response to treatment. Following a risk assessment, the authors identified a list of CQAs for AAVs. When developing an AAV production process, the authors determined specifications for AAV CQAs, including viral and infectious titres, the presence of replication-competent AAVs, the percentage of empty capsids, and residual impurities (proteins, plasmid DNA, and residual host-cell DNA). A comprehensive risk assessment was conducted to determine the quality target product profile for an AAV-based gene therapy product for Duchenne muscular dystrophy. The authors listed the CQAs, developed the basic requirements for the applicable analytical procedures, and established the CQA specifications for the gene therapy product.CONCLUSIONS. The use of QbD principles and risk-based approaches is an important step in CQA identification during the development of gene therapy products. The QbD methodology facilitates drafting new regulatory standards for the evaluation of the safety and efficacy of gene therapy products and helps with the development and commercial-scale manufacturing of such products.
АНАЛІЗ НОРМАТИВНО-ПРАВОВИХ АКТІВ ЩОДО АКТУАЛЬНИХ ПИТАНЬ ПІДГОТОВКИ ФАХІВЦІВ ГАЛУЗІ ОХОРОНА ЗДОРОВ’Я ТА СОЦІАЛЬНЕ ЗАБЕЗПЕЧЕННЯ: ВИКЛИКИ ТА ПЕРСПЕКТИВИ
Authors: Кучин Ю. Л. , Власенко О. М. , Кучеренко І. І. , Микитенко П. В. , Мельник О. М. 
Published: February 2025

Стаття присвячена актуальним питанням підготовки фахівців галузей охорона здоров’я та соціальне забезпечення. Також проаналізовано сучасні законодавчі та підзаконні акти, які регламентують їх підготовку. Першим викликом для медичної освіти України стала пандемія COVID-19, заклади вищої медичної освіти змушені були швидко пристосовуватися до нових умов навчання, шукати нові форми, засоби, методи та ресурси для забезпечення якісного надання освітніх послуг. Наступним викликом стало введення воєнного стану на території України 24 лютого 2024 р. Внаслідок цих подій вітчизняна система вищої медичної освіти знаходиться на шляху складних випробувань та вимушених трансформацій. Приєднання до системи європейської статистики вимагає реструктуризації української системи обліку підготовки фахівців.
За останній рік було прийнято низку законів та постанов, що впливають та регулюють підготовку майбутніх фахівців, що надаватимуть медичну допомогу. До них належать Закон України “Про внесення змін до деяких законів України щодо розвитку індивідуальних освітніх траєкторій та вдосконалення освітнього процесу”, Постанова Кабінету міністрів України “Про внесення змін до переліку галузей знань і спеціальностей, за якими здійснюється підготовка здобувачів вищої та фахової передвищої освіти” та Концептуально-референтна рамка цифрових компетентностей працівників сфери охорони здоров’я та забезпечення розвитку інформаційної культури, цифрової грамотності (цифрової освіченості), кібербезпеки і кібергігієни працівників сфери охорони здоров’я.
Відтак Урядом схвалено новий перелік галузей знань та спеціальностей, які оновлено з метою узгодження з міжнародними стандартами. Формування індивідуальної освітньої траєкторії здобувачів вищої освіти попередньо регламентуватиметься Наказом Міністерства освіти і науки України “Про затвердження Переліку спеціальностей, здобуття ступеня освіти з яких необхідне для доступу до професій, для яких запроваджено додаткове регулювання”.
ПРАКТИКА “ПЕРШІ ДНІ ДИТИНИ У ЗЗСО” ЯК СКЛАДОВА ПРОФЕСІЙНОЇ ПІДГОТОВКИ МАЙБУТНІХ УЧИТЕЛІВ ПОЧАТКОВИХ КЛАСІВ
Authors: Толмачова І. М. , Потапова Н. В. 
Published: February 2025

Актуальність статті зумовлена важливістю розв’язання протиріччя між необхідністю здійснювати практичну підготовку майбутніх учителів початкових класів в умовах сучасних викликів та відсутністю належного організаційного та змістового забезпечення даного напряму діяльності у педагогічних ЗВО.
У статті узагальнено досвід організації та проведення практики “Перші дні дитини у ЗЗСО” зі здобувачами першого (бакалаврського) рівня вищої освіти галузі знань 01 Освіта/Педагогіка за спеціальністю 013 Початкова освіта у Комунальному закладі “Харківська гуманітарно-педагогічна академія” Харківської обласної ради через детальний опис перебігу практики в ході організаційного, практичного та підсумкового етапів роботи.
Представлено результати анкетування майбутніх фахівців після його проведення. У ході анкетування з’ясовано, що ефективність адаптації першокласників до шкільного навчання залежить від багатьох факторів – психологічних, методичних, дидактичних, соціальних тощо. Студенти продемонстрували уміння аналізувати психологічний стан і рівень підготовки дітей до школи, виокремлювати професійно значущі знання, вміння та якості, необхідні для успішної адаптації та навчання першокласників, бачити суперечності, труднощі сучасного освітнього процесу та знаходити шляхи їх подолання.
Авторами відстежено взаємозалежність організаційної та змістової складових навчальної практики, підкреслено зв’язок практики “Перші дні дитини в ЗЗСО” з іншими освітніми компонентами, з освітньою програмою спеціальності “Початкова освіта”.
Запропоновано завдання, які доречно додати до змісту відповідного силабуса й запропонувати майбутнім учителям за результатами зворотного зв’язку в наступному навчальному році.
Analysis exhaust emissions (CO, CO<sub>2</sub> and HC) on FI motorcycle with variations in ignition timing, injector timing and fuel variations
Authors: Purwanto W , Firmansyah M S , Maksum H , Arif A , Setiawan M Y 
Published: February 2025

This study discusses changes in the value of CO,CO2 and HC levels of exhaust emission on FI Motorcycle with the use ECU Programmable (4 variation of ignition timing and injector timing) and use 3 variations fuel in every treatment. The purpose of this study was to determine how much influence the use ECU Programmable and variation of fuel on exhaust emissions produced by the research object. This type of research is experimental research. The object of this research is the FI Motorcycle. The lowest CO and HC level produced by FI Motorcycle with the use of a Programmable ECU is obtained in the treatment at ignition timing of 7°BTDC and injector timing 350°ATDC using Gasohol E30 fuel, while the lowest CO2 level is obtained at the ignition timing treatment of 3°BTDC and when injector timing 350°ATDC uses Pertamax Turbo fuel, where the results obtained show significant changes after going through a comparative test using the T test with a significant level of 5%.
Socio-environmental Study and Ecological Footprint in the Mixtec Community of El Calvario
Authors: Villanueva-Solano José A. , Juárez-López Ana L. , Brito-Carmona Rosa M. , Arellano-Wences Hilda , FORERO FORERO ANGELA VICTORIA , Toribio-Jiménez Jeiry 
Published: February 2025

Subject: Currently, it is very difficult for communities to receive funding for their development, as they often lack efficient evaluations or significant impact to support such funding. Therefore, it is vitally important for research to help make these communities visible by applying instruments that describe their situation. In this sense, two of the most important tools are the socio-environmental diagnosis and the ecological footprint, which are rarely determined in various communities.
Methodology: To determine this, Likert-type surveys and interviews with key stakeholders were conducted to identify areas for improvement within the community.
Results: The most relevant findings were the need to pave the road leading to the community, which hinders marketing and increases the cost of its products and/or the residents' labor. Another issue is the management, disposal, and elimination of waste. The greatest waste identified is the bagasse from agave, which must be valorized. Additionally, efficient water collection systems are needed, and finally, there is a lack of medicines and an available family doctor.
Implications: As a result of this study, various strategies are proposed to provide continuity to the areas of opportunity identified in the El Calvario community.
Conclusions: It is important to listen to the community's context so that they become the promoters of change, without harming the environment and nature.
OPTIMALISASI PENGELOLAAN KEUANGAN DESA UNTUK PEMBANGUNAN BERKELANJUTAN : STUDI KASUS DESA PASIRTALAGA
Authors: Gun Gumilar Gun , Sugiarto Dimas , Elyas Ahmad Mudzaki , Atika Ayu 
Published: February 2025

The Village Fund in Indonesia launched in 2015 through the policy of Village Law No. 6/2014 provides a strong impetus for villages to independently manage their financial resources to achieve inclusive, prosperous, and environmentally friendly development. This study aims to analyse how village financial management can be optimised to support sustainable development. The theory used is sustainable development which consists of three main pillars, namely economic, social, and environmental, developed by the Brundtland Commission (1987). This research uses a qualitative approach with a case study method that aims to explore efforts to optimise village financial management in supporting sustainable development in Pasirtalaga Village. The case study was chosen because it provides an in-depth description of the local dynamics of village financial management that is unique and specific to the context of Pasirtalaga Village. The results showed that optimised village financial management can improve the efficiency of allocating funds to development projects that can improve the economy, increase social welfare and maintain sustainability.
Analysis and validation of biomarkers and immune cell infiltration profiles in unstable coronary atherosclerotic plaques using bioinformatics and machine learning
Authors: Jin Pengyue , Zhang Shangyu , Yang Li , Zeng Yujie , Li Yongguo , Tang Renkuan 
Published: February 2025

IntroductionDecreased stability of coronary atherosclerotic plaques correlates with a heightened risk of acute coronary syndrome (ACS). Thus, early diagnosis and treatment of unstable plaques are imperative in averting adverse cardiovascular events. This study aims to identify diagnostic biomarkers for unstable coronary atherosclerotic plaques and investigate the role of immune cell infiltration in their formation.MethodsThe datasets GSE163154 and GSE111782, obtained from the gene expression omnibus (GEO) database, were amalgamated for bioinformatics analysis, using the dataset GSE43292 as a test set. Sequentially, we performed principal component analysis (PCA), differential gene expression analysis, enrichment analysis, weighted gene co-expression network analysis (WGCNA), utilized a machine learning algorithm to screen key genes, conducted receiver operating characteristic (ROC) curve analysis and nomogram model to assess biomarker diagnostic efficacy, validated the biomarkers, and analyzed immune cell infiltration.ResultsIn conclusion, enrichment analyses demonstrate that genes are significantly enriched in inflammatory and immune-related pathways. We identified HSPA2 and GEM as key genes and validated them experimentally. Significant differences existed in immune cell infiltration between subgroups. Additionally, HSPA2 and GEM showed significant associations with a wide range of immune cells.DiscussionHSPA2 and GEM can function as diagnostic biomarkers for unstable coronary atherosclerotic plaques. In combination with immune cell infiltration analyses, our study provides new insights into the future study of unstable plaque occurrence and molecular mechanisms.
Low-loss power management strategy for weak and low-frequency biomechanical energy harvesting for new generation wearable electronics
Authors: Li Weilu , Huang Yongcan , Liu Chunhua , Valencia Agnes , Daoud Walid A. 
Published: February 2025

IntroductionAmidst the rapidly growing development of wearable electronics, their dependence on external power sources increases the power expense while leading to interruptions of their operation during charging. Biomechanical energy harvesters offer a promising solution for self-powered wearable electronics by converting waste kinetic energy to electricity. Despite successful efforts in advancing their power outputs from μW to mW, several challenges persist, including low output current at the μA-level, high internal impedance in the GΩ-level, and AC outputs, restricting their practical applications. Conventional power management circuits are commonly utilized in high-frequency harvesters without adequate consideration of the energy loss that incurs, potentially leading to circuit failure when used in low-frequency harvesters with a lower power output.MethodsHere, we introduce a low-loss power management circuit (L-PMC) that functions under low-frequency conditions to facilitate biomechanical energy harvesting.ResultsOur innovative two-stage energy transfer strategy boosts the energy extraction efficiency to 42.24%, breaking previous records. With an energy transfer efficiency of 30.59%, L-PMC can charge a battery from 1.9 V to 2.4 V in just 10 min.DiscussionMoreover, the integration of passive current amplification tripled charge accumulation and energy storage, representing 207% enhancement in energy transfer efficiency, presenting a versatile and universal approach to low-frequency biomechanical energy harvesting for new generation wearable electronics.
Geochemical factors influencing the phosphorus mobility in Konza prairie grassland and agriculture-dominated soils in north-eastern Kansas
Authors: Banerjee Protik , Kulkarni Harshad V. , Veach Allison M. , Nagaraja Thiba , Pathak Pousali , Das Suprem R. , Datta Saugata 
Published: February 2025

IntroductionThe availability and mobility of phosphorus (P) in soils play a crucial role in effectively managing agricultural activities and maintaining healthy soils. Several parameters including soil texture, pH, elemental and mineralogical composition, moisture content, and soil organic matter (SOM) are crucial in controlling the movement of P in soils. This study focuses on assessing geochemical properties of soils from a pristine prairie grassland and an agriculturally dominated land, and their influence on soil P mobility.MethodsSurface soils were collected from two locations, Konza Prairie Biological Station (KBPS) located in Manhattan (Kansas) which is a native grassland ecosystem, and agricultural land in town of Hays (Kansas).ResultsResults showed that the KPBS soils contained lower water-extractable phosphate (PO43—) concentrations (0.2 ± 0.7 mg/kg) than soils from Hays (1.3 ± 2.4 mg/kg). Bio-available P measured as Bray-P were also lower in KPBS (14.3 ± 7.0 mg/kg) relative to Hays (23.0 ± 23.7 mg/kg). Soils from both the sites contained water-extractable calcium, magnesium and potassium as a primary soluble component likely from carbonate minerals in these calcareous soils. The SOM concentrations measured as loss on ignition (LoI) were greater in KPBS (9.9% ± 1.8%) relative to Hays (5.3% ± 1.7%). Water extractable soil organic carbon (WE-SOC) concentrations were also greater for KPBS (651 ± 274 mg/kg) relative to Hays (288 ± 267 mg/kg). Optical spectroscopic analyses using absorbance and fluorescence properties revealed that the water-extractable SOM in these soils was mainly of terrestrial origin, plant-derived, aromatic, and contained humic-like substances. The intensities of fluorescence peaks A, C, and M, and specific UV absorbance at 254 nm (SUVA 254) of both soils correlate strongly with the Bray-P concentrations, indicating that the source of SOM plays a vital role in controlling soil P mobility.ConclusionThese findings indicate that natural prairie grassland soils contained lower P concentrations that are primarily insoluble in water and associated with humic and fulvic-like SOM.
Disruption of the moonlighting function of CTF18 in a patient with T-lymphopenia
Authors: Sertori Robert , Truong Billy , Singh Manoj K. , Shinton Susan , Price Rachael , Sharo Andrew , Shultes Paulameena , Sunderam Uma , Rana Sadhna , Srinivasan Rajgopal , Datta Sutapa , Font-Burgada Joan , Brenner Steven E. , Puck Jennifer M. , Wiest David L. 
Published: February 2025

IntroductionNewborn screening for immunodeficiency has led to the identification of numerous cases for which the causal etiology is unknown.MethodsHere we report the diagnosis of T lymphopenia of unknown etiology in a male proband. Whole exome sequencing (WES) was employed to nominate candidate variants, which were then analyzed functionally in zebrafish and in mice bearing orthologous mutations.ResultsWES revealed missense mutations in CHTF18 that were inherited in an autosomal recessive manner. CTF18, encoded by the CHTF18 gene, is a component of a secondary clamp loader, which is primarily thought to function by promoting DNA replication. We determined that the patient’s variants in CHTF18 (CTF18 R751W and E851Q) were damaging to function and severely attenuated the capacity of CTF18 to support hematopoiesis and lymphoid development, strongly suggesting that they were responsible for his T lymphopenia; however, the function of CTF18 appeared to be unrelated to its role as a clamp loader. DNA-damage, expected when replication is impaired, was not evident by expression profiling in murine Chtf18 mutant hematopoietic stem and progenitor cells (HSPC), nor was development of Ctf18-deficient progenitors rescued by p53 loss. Instead, we observed an expression signature suggesting disruption of HSPC positioning and migration. Indeed, the positioning of HSPC in ctf18 morphant zebrafish embryos was perturbed, suggesting that HSPC function was impaired through disrupted positioning in hematopoietic organs.DiscussionAccordingly, we propose that T lymphopenia in our patient resulted from disturbed cell-cell contacts and migration of HSPC, caused by a non-canonical function of CHTF18 in regulating gene expression.