bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kajian Poskolonial dalam Novel Babad Kopi Parahyangan Karya Evi Sri Rezeki dan Pemanfaatannya sebagai Alternatif Materi Pembelajaran Sastra di SMA
Authors: Misgiarti Nafisah , Taufiq Akhmad , Murti Fitri Nura 
Published: July 2024

Kajian poskolonial digunakan untuk menyingkap sejarah kolonialisme dan mengkaji sebuah relasi yang tidak setara dalam suatu sistem masyarakat. Penelitian ini membahas tentang praktik dominasi dan resistensi golongan subaltern dalam novel Babad Kopi Parahyangan. Penelitian ini merupakan penelitian kualitatif dengan rancangan deskriptif interpretatif. Sumber data dalam penelitian ini adalah naskah novel BKP karya Evi Sri Rezeki, artikel, jurnal, dan buku yang berkaitan dengan sejarah tanam paksa, dan silabus kurikulum 2013 revisi 2018 pada tingkat SMA kelas XII. Data penelitian ini berupa kutipan peristiwa dalam bentuk kata-kata, kalimat, dan paragraf yang mengindikasikan adanya dominasi dan resistensi kaum subaltern dalam novel BKP. Teknik pengumpulan data yang digunakan ialah teknik dokumentasi. Penelitian ini menggunakan metode deskriptif analisis. Berdasarkan data yang diperoleh, praktik dominasi golongan superior terhadap golongan subaltern dalam novel BKP yaitu dominasi politik dan ekonomi. Resistensi yang dilakukan oleh golongan subaltern yaitu resistensi aktif dan pasif. Dominasi ekonomi yang dilakukan pihak superior dilakukan berdasarkan konsensus karena golongan subaltern menyadari praktik dominasi ekonomi yang dialami, tetapi masih belum memiliki keinginan kuat untuk melawan. Semua tindakan resistensi aktif dilakukan secara terencana dan berhasil memberikan dampak nyata. Resistensi pasif dalam novel BKP berupa rasa dendam serta keinginan untuk merdeka dan melawan sebagai bentuk pertahanan identitas diri.
Angelman syndrome in Poland: current diagnosis and therapy status – the caregiver perspective – a questionnaire study
Authors: Agata Suleja , Katarzyna Milska-Musa , Łukasz Przysło , Marzena Bednarczyk , Marcin Kostecki , Dominik Cysewski , Paweł Matryba , Anna Rozensztrauch , Michał Dwornik , Marcin Opacki , Robert Śmigiel , Lukasiewicz Kacper 
Published: July 2024

Abstract

Background Angelman syndrome (AS) is a rare neurodevelopmental disease caused by imprinting disorders that impede the production of the ubiquitin E3A ligase protein (UBE3A). AS affects multiple systems, with the main symptoms including epilepsy, psychomotor disorders and speech development disorders. To date, no study has been conducted in the Polish population to verify the condition's diagnosis and treatment process.
Results Seventy patients with the median age of 60 months were included into the analysis. 80% of patients were diagnosed with deletion, 19.9% with a mutation of UBE3A gene, 4.3% with paternal uniparental disomy (UPD) and 2.8% with an imprinting defect.
The mean age of first symptoms was 5 months, while the mean age of diagnosis was 29 months (earliest in deletion group at 23 months), and the median duration of diagnosis process was 7 months. The average time to a clinical geneticist appointment was 3 months. 37.9% of the patients initially received a different diagnosis. Epileptic seizures were present in 88.6% of the individuals. 98.6% of the studied group were under care of a pediatric neurologist, 47.1% of a gastroenterologist. A ketogenic diet was used in 7.1% of patients. Caregivers identified finding a specialist suitable for AS patients and access to genetic testing as the biggest problems.
Conclusions The care of patients with AS in Poland is carried out according to the European and world standards, however there is an impeded access to clinical geneticist, and the knowledge about rare diseases among primary healthcare physicians could be improved. Moreover, access to AS care specialists and coordination of care is limited. There is a need for creation a specialized centers and databases for AS patients.
Antarctic geothermal soils exhibit an absence of regional habitat generalist microorganisms
Authors: McDonald Ian , Noell Stephen , Abbaszadeh Jaber , Richards Huw , Vincent Marie Labat Saint , Lee Charles , Herbold Craig , Stott Matthew , Cary Stephen 
Published: July 2024

Abstract

Active geothermal systems are relatively rare in Antarctica and represent metaphorical islands ideal to test microbial dispersal. In this study, we tested the macroecological concept that high dispersal rates result in communities being dominated by either habitat generalists or specialists by investigating the microbial communities on four geographically separated geothermal sites on three Antarctic volcanoes (Mts. Erebus, Melbourne and Rittman). We found that the microbial communities at higher temperature sites (Tramway Ridge on Erebus and Rittmann) were unique from each other and were dominated by a variety of novel Archaea from class Nitrososphaeria, while lower temperature sites (Western Crater on Erebus and Melbourne) had characteristically mesophilic communities (Planctomycetes, Acidobacteriota, etc) that were highly similar. We found that 97% of the detected microbial taxa were regional habitat specialists, with no generalists, with community assembly driven by high dispersal rates and drift (25 and 30% of community assembly, respectively), not environmental selection.
Efficacy and Safety of Vosoritide for Achondroplasia: A Systematic Review and Dose- Related Meta-Analysis, 2024 Article type: Systematic review and Dose-related Meta-analysis
Authors: Khan Misha , Akhtar Syed Muhammad Muneeb , Zulfiqar Eeshal , Sabir Hafsa , Amer Samar A. 
Published: July 2024

Abstract

Background
Achondroplasia, the most common form of dwarfism, poses significant medical and psychosocial challenges. Vosoritide, a new C-type natriuretic peptide analog, has shown promise in treating achondroplasia by helping bones grow. Therefore, we conducted this study to examine the effect of different vosoritide doses on height from baseline (z-score), annualized growth velocity (AGV), the upper-to-lower body segment ratio (ULBR), and side effects. changes in growth velocity and the frequency of adverse effects.
Methods
This systematic review and dose-related meta-analyses follow the PRISMA guidelines, which meticulously screen and extract randomized controlled trials (RCTs) from four databases until April 2024 involving 220 patients. We used pairwise meta-analyses and assessed quality using the Cochrane Risk of Bias Tool.
Results
Higher Vosoritide doses (15 µg/kg or 15–30 µg/kg) showed significantly greater improvements in height z-scores compared to lower doses (2.5 µg/kg or 7.5 µg/kg) or placebo. We observed similar trends for AGV. The 15 µg/kg dose displayed a significantly greater increase compared to both 2.5 µg/kg and 7.5 µg/kg. Interestingly, no significant difference was found between the 15 µg/kg and 30 µg/kg groups, suggesting a possible plateau effect at higher doses. Importantly, both the 15 µg/kg and 15–30 µg/kg groups demonstrated statistically significant improvements in growth velocity compared to placebo. While most comparisons showed no significant changes in ULBR, one study reported a small increase in ULBR with the 15 µg/kg dose compared to the 2.5 µg/kg dose. Adverse effects were mild to moderate across all studies, with no severe effects reported.
Conclusion
Vosoritide improves achondroplasia growth in a dose-dependent manner. Higher doses (15 µg/kg or 15–30 µg/kg) significantly increased height and growth velocity compared to lower doses or placebo. All adverse effects were mild to moderate. However, the impact on the ULBR and adverse effects require further investigation.
Orphan receptor-GPR52 inverse agonist efficacy in ameliorating chronic stress-related deficits in reward motivation and phasic accumbal dopamine activity in mice
Authors: Pryce Christopher , Zhang Chenfeng , Kúkeľová Diana , Sigrist Hannes , Hengerer Bastian , Kratzer Ramona , Mracek Philipp , Omrani Azar , von Heimendahl Moritz 
Published: July 2024

Abstract

Reward processing dysfunctions e.g., anhedonia, apathy, are common in stress-related neuropsychiatric disorders including depression and schizophrenia, and there are currently no established therapies. One potential therapeutic approach is restoration of reward anticipation during appetitive behavior, deficits in which co-occur with attenuated nucleus accumbens (NAc) activity, possibly due to NAc inhibition of mesolimbic dopamine (DA) signaling. Targeting NAc regulation of ventral tegmental area (VTA) DA neuron responsiveness to reward cues could involve either the direct or indirect – via ventral pallidium (VP) – pathways. One candidate is the orphan G protein-coupled receptor GPR52, expressed by DA receptor 2 NAc neurons that project to VP. In mouse brain-slice preparations, GPR52 inverse agonist (GPR52-IA) attenuated evoked inhibitory postsynaptic currents at NAc-VP neurons, which could disinhibit VTA DA neurons. A mouse model in which chronic social stress leads to reduced reward learning and effortful motivation was applied to investigate GPR52-IA behavioral effects. Control and chronically stressed mice underwent a discriminative learning test of tone-appetitive behavior-sucrose reinforcement: stress reduced appetitive responding and discriminative learning, and these anticipatory behaviors were dose-dependently reinstated by GPR52-IA. The same mice then underwent an effortful motivation test of operant behavior-tone-sucrose reinforcement: stress reduced effortful motivation and GPR52-IA dose-dependently restored it. In a new cohort, GRABDA-sensor fibre photometry was used to measure NAc DA activity during the motivation test: in stressed mice, reduced motivation co-occurred with attenuated NAc DA activity specifically to the tone that signaled reinforcement of effortful behavior, and GPR52-IA ameliorated both deficits. These findings: (1) Demonstrate preclinical efficacy of GPR52 inverse agonism for stress-related deficits in reward anticipation during appetitive behavior. (2) Suggest that GPR52-dependent disinhibition of the NAc-VP-VTA-NAc circuit, leading to increased phasic NAc DA signaling of earned incentive stimuli, could account for these clinically relevant effects.
Evaluation of Batch-To-Batch Variability and Efficacy Of IPSC-Derived Mesenchymal Stromal Derived Extracellular Vesicles in Mitigating Osteoarthritis Associated Inflammation In Vitro
Authors: Palamà Maria Elisabetta Federica , Gorgun Cansu , Rovere Matteo , Shaw Georgina M. , Reverberi Daniele , Formica Matteo , Quarto Emanuele , Barry Frank , Murphy Mary , GENTILI CHIARA 
Published: July 2024

Abstract

Background
Mesenchymal stromal cell-derived extracellular vesicles (MSC-EVs) hold promise as a cell-free therapy for various diseases, including osteoarthritis (OA), due to their immunomodulatory and anti-inflammatory properties. However, the need for large-scale expansion of MSCs to obtain MSC-EVs for clinical use often leads to senescence-related changes and loss of stem-like properties. In this scenario, induced pluripotent stem cell (iPSC)-derived MSCs (iMSCs) offer the unique opportunity to address obstacles associated with traditional MSC-based therapies.
Methods
In this study, a xeno-free (XFS) medium was used for long-term expansion of both MSCs and iMSCs, and subsequent isolation of MSC- and iMSC-derived EVs. Characterization of both cells and EVs was conducted across different passages, and the anti-inflammatory potential of EVs and iEVs was assessed using an in vitro model of osteoarthritis.
Results
Long-term expansion of MSCs resulted in cellular senescence and a reduction in trilineage differentiation capacity by passage five, accompanied by diminished anti-inflammatory properties of EVs. On the other hand, iMSCs exhibited batch-to-batch variability in differentiation potential and the biological properties of iEVs. However, the effects of iMSC-EVs are prolonged compared to MSC-EVs, providing a prolonged window of activity for therapeutic purposes.
Conclusions
While iMSC-EVs demonstrated prolonged effects compared to MSC-EVs, heterogeneity among iMSC batches present significant challenges in utilizing iMSC-derived EVs as a reliable tool for clinical OA treatment. Addressing these limitations is crucial for advancing the translational potential of iPSC-derived therapies in the management of osteoarthritis and other diseases.
Modeling green infrastructure as a flood mitigation strategy in an urban coastal area
Authors: Kriebel Martijn , Liu Ting , Ramirez-Marquez Jose E. , Prigiobbe Valentina 
Published: July 2024

Abstract

Urban areas can be seriously disrupted by flooding after heavy rain events. Therefore, several strategies based on grey infrastructure have been implemented over the years to mitigate the impact of significant rainfalls and make urban areas more resilient against flash floods. Green Infrastructure (GI) is an environmentally appropriate alternative which can reduce the amount of stormwater delivered to a drainage system within an urban area while mitigating the contamination carried with it. To date, however, relatively little attention has been paid to the public acceptance and the presence of shallow aquifers before the implementation of GI measures. This paper presents a framework to assess the social and technical feasibility of GI in a coastal urban area prone to severe flooding. The method consists of the assessment of potential scenarios upon the evaluation of government acceptance coupled with a stormwater management model.The city of Hoboken (NJ) located near the estuary of the Hudson river was selected as an urban laboratory given its vulnerability to flooding and the availability of data.A stormwater management model was developed and used to simulate various GI options to minimize stormwater runoff.Outcomes from the interviews indicate positive feedback for GI implementation, but no specific alternatives could be identified. Based on generally accepted GI measures, i.e., right-of-way storage, resiliency parks, and green roofs, urban hydrology models were implemented. Simulation results show that all of the considered alternatives are effective in reducing runoff volumes for rainfall events of less than 1-year recurrence interval. However, they do not mitigate the negative impact of heavier rain events due to the limited storage as a consequence of site size and the shallow aquifer within the coastal urban area.
Painful legs and moving toes with anti-hu antibody positive paraneoplastic syndrome: a case report and review of literature
Authors: Liu Jun , Tan Weihong , Li Ying , Peng Zhanghui , Hu Zhenzhu 
Published: July 2024

Abstract

Background
Rare and poorly understood, Painful legs and moving toes (PLMTS)manifests as abnormal movements of the toes and moderate to severe leg and foot pain. The exact underlying pathophysiology is not clear. Its incidence is unknown. Here, we report a female patient that was initially misdiagnosed with Sjogren's syndrome by a rheumatologist, but eventually correctly diagnosed with Anti-hu antibody positive paraneoplastic syndrome.
Case presentation:
A 66-year-old female suffered from severe pain in both lower limbs for three months, accompanied by involuntary and irregular movements in her ankles and feet. She also complained of dry mouth. She was initially diagnosed with painful leg and moving toes syndrome provoked by Sjögren's syndrome-related peripheral neuropathy and received corticosteroid pulse therapy. However, the patient's symptoms continued to worsen over time.Follow-up examinations revealed that the patient had anti-Hu antibody IgG positivity in both her blood and cerebrospinal fluid samples, together with a tumor in the right ovary. The patient then underwent regular chemotherapy for ovarian cancer and her neurological symptoms were partially relieved during the follow-up. In our case, electroencephalography and brain MRI all revealed unremarkable findings: in clinical practice, this could lead to misdiagnosis with psychogenic conditions. Our patient was almost misdiagnosed as primary Sjogren’s syndrome by a rheumatologist as well. Consequently, we consider that clinicians should be aware of this unique disorder in order to avoid misdiagnosis.
Conclusion
PLMTS can be seen in anti-hu antibody positive paraneoplastic syndrome.
Multiple cryptic lineages and restricted gene flow in the decorator worm Diopatra cuprea
Authors: Ziegler Adam J. , Bell Tina M. , Berke Sarah K. , Strand Allan E. , Sotka Erik E. 
Published: July 2024

Abstract

The decorator worm Diopatra cuprea Bosc, 1802 (Annelid; Polycheate; Onuphidae) is an ecosystem engineer within high-salinity estuaries of the southern and eastern United States. A previous study revealed five relatively deep mitochondrial lineages across its wide geographic distribution. Here, we explore geographic distribution and mitonuclear concordance of these mitochondrial lineages with single nucleotide polymorphisms (SNPs) genotyped using RADseq. We genotyped 3,162 SNPs from 233 D. cuprea and detected four deep lineages in the nuclear genome: a northern US clade (Massachusetts), a single nuclear clade within mid-Atlantic populations (i.e., Virginia south through northeastern Florida), a southeastern Florida clade and a Gulf of Mexico clade. There was mitonuclear concordance within most individuals, with the notable exception of two mitochondrial clades within a single mid-Atlantic nuclear clade. Thus, there appear to be four cryptic lineages of D. cuprea that suggest four distinct species that rarely hybridize. Within the mid-Atlantic lineage, we detected increasing genetic isolation of populations with increasing geographic distance, a pattern consistent with low dispersal of D. cuprea larvae. Cryptic diversity within the D. cuprea complex is consistent with other common and geographically widespread annelid and Diopatra species that are now being revealed using high-throughput sequencing.
MFF budding from mitochondria regulates melanosome size and maturation
Authors: Rebelo Ana Magalhães , Maracani Aurora , Greco Samuele , Bello Federica Dal , Santorelli Lucia , Gerdol Marco , Pallavicini Alberto , Schiavon Sara , Goff Philip , Scorrano Luca , Sviderskaya Elena , Grumati Paolo , Giacomello Marta 
Published: July 2024

Abstract

Melanosomes are lysosome-related organelles that produce and accumulate melanin. Melanosome maturation is regulated by their association with mitochondria and requires the export and recycling of unneeded cargo via tubular carriers and fission, the mechanisms of which are unknown. Here we show that melanosome fission requires the outer mitochondrial membrane protein mitochondrial fission factor (MFF). We retrieved MFF on melanosomes at different stages of maturation and at early melanosome fission sites: upon downregulation of MFF, but not of the dynamin related protein 1 (DRP1) that executes mitochondrial fission, early melanosomes enlarged, intracellular melanin accumulated and melanosome lumen catabolism increased. The MFF interactome in melanocytes posited a role for a complex network of cytoskeletal factors in its activity. Indeed inhibition of actin nucleation was sufficient to curtail the effects of MFF silencing on melanosomes. Our data unveil an extramitochondrial role for MFF in the regulation of melanosome morphology and maturation that is independent of DRP1 but requires actin polymerization and nucleation factors.
Prevalence, Knowledge and Risk Factors of Medical Student Syndrome Among Medical Students at the university of Khartoum, March 2022
Authors: Elobaid Mohamed Atif Abdelgadir , Abdelgyoum Hafeia Abdelrazig Elhussien , Omer Lina M. , Mohammed Rooa , Musa Muawia E. , Taha Aya M. 
Published: July 2024

Abstract

Introduction: Medical Student Syndrome is defined as development of fears and symptoms of an illness related to a disease which a medical student is studying at the time without actually having the disease. The study aimed to measure the prevalence of medical student syndrome, to assess students knowledge of MSS and to identify the association between possible related risk factors [gender, year of study, academic performance, smoking, family medical profession, personal and family psychiatric illness, repeating, addiction and Googling symptoms] and MSS prevalence and some other characteristics [e.g. strength and duration].
Method: This cross-sectional study was conducted on medical students at university of Khartoum, Sudan. A total of 333 students were selected via stratified proportionate simple random sampling. They completed an electronically disseminated non-standardized questionnaire. Frequencies were calculated via SPSS version 23 and Chi Square tests were done to identify the association between different risk factors and MSS..
Results: 357 medical students participated in this study. 71.1% of them were females. The prevalence of MSS was 62.2%. 3rd and 4th year students recorded the highest rates of prevalence among students. 53.5% of students knew about MSS. 44.1% of students with MSS thought that they had a real disease while experiencing the syndrome. Most of the students who experienced MSS [92.8%] had mild to moderate symptoms, mostly in the cardiovascular system. These symptoms lasted for minutes in the majority [45%]. 19.8% of students with MSS sought medical help. Regarding the risk factors associated with MSS, there is a significant difference associated with the following risk factors [ Sex P=0.008 , Family psychiatric history P=0.009, Googling Symptoms P=0.000 , being in the middle years of study P = 0.000 , being in clinical years P=0.014 , as the P value <0.05 ]..
Conclusions: There is a disparity between MSS prevalence and Knowledge. Some students seek medical help because of it so further efforts are needed to address it to decrease worries and load on the health facilities. Further research is needed to identify and confirm risk factors related to this syndrome.
Rubber Mandrel and Internal Pressure Effects on Thin-Walled Tube Bending: A Comparative Study
Authors: Sayar Majid Askari , Gerdooei Mahdi , Eipakchi Hamidreza , Nosrati Hasan Ghafourian 
Published: July 2024

Abstract

Tube bending plays a crucial role in various industrial sectors, ranging from aerospace, military, and petrochemical to automotive manufacturing. However, a persistent challenge in this process is the occurrence of springback, which disrupts the intended shape of the bent tubes and affects product assembly. While previous research has primarily focused on predicting and managing springback using simplified biaxial stress assumptions, the influence of radial normal stress (internal pressure) has often been overlooked, particularly in modern bending techniques like hydroforming and rubber pad forming. In this study, a comprehensive investigation into the impact of internal pressure on springback during tube bending was undertaken, employing analytical, numerical, and experimental methodologies. The utilization of a rubber mandrel in thin-walled tube bending effectively reduces springback angles by up to 9.86% compared to bending without it. The numerical simulation demonstrates stronger agreement with experimental data, highlighting the importance of internal pressure generated by the mandrel's contact stress. This pressure contributes to increasing the plastic strain level, extending the plastic skin, and removing the elastic core, subsequently reducing springback.