bluuebunny/crossref_metadata_2025_split huggingface dataset:
Melatonin attenuates neurogenic pulmonary edema via the regulation of inflammation and apoptosis after subarachnoid hemorrhage in rats
Authors: Chen Jingyin , Qian Cong , Duan Hongyu , Cao Shenglong , Yu Xiaobo , Li Jianru , Gu Chi , Yan Feng , Wang Lin , Chen Gao 
Published: September 2015

AbstractNeurogenic pulmonary edema (NPE) is a serious non‐neurological complication that can occur after a subarachnoid hemorrhage (SAH) and is associated with decreased survival and a poor neurological outcome. Melatonin is a strong antioxidant that has beneficial effects against SAH in rats, including reduced mortality and reduced neurological deficits. The molecular mechanisms underlying these clinical effects in the SAH model, however, have not been clearly identified. This study was undertaken to determine the influence of melatonin on SAH‐induced NPE and the potential mechanism of these effects using the filament perforation model of SAH in male Sprague Dawley rats. Either melatonin (150 mg/kg) or a vehicle was given via an intraperitoneal injection 2 hr after an SAH induction. Lung samples were extracted 24 hr after SAH. The results show that the melatonin treatment attenuated SAH‐induced NPE by preventing alveolar–capillary barrier dysfunctions via inhibiting the disruption of tight junction proteins (ZO‐1 and occludin). Moreover, the treatment downregulated the levels of mature interleukin (IL) ‐1β, myeloperoxidase (MPO), and matrix metallopeptidase (MMP) 9 expression/activation, which were increased in the lung; also, melatonin treatment improved neurological deficits. Furthermore, the melatonin treatment markedly reduced caspase‐3 activity and the number of TUNEL‐positive cells in the lung. Taken together, these findings show that administration of melatonin attenuates NPE by preventing alveolar–capillary barrier dysfunctions via repressing the inflammatory response and by anti‐apoptosis effects after SAH.
Chloroplast phylogenomics resolves key relationships in ferns
Authors: Lu Jin‐Mei , Zhang Ning , Du Xin‐Yu , Wen Jun , Li De‐Zhu 
Published: September 2015

AbstractStudies on chloroplast genomes of ferns and lycophytes are relatively few in comparison with those on seed plants. Although a basic phylogenetic framework of extant ferns is available, relationships among a few key nodes remain unresolved or poorly supported. The primary objective of this study is to explore the phylogenetic utility of large chloroplast gene data in resolving difficult deep nodes in ferns. We sequenced the chloroplast genomes from Cyrtomium devexiscapulae (Koidz.) Ching (eupolypod I) and Woodwardia unigemmata (Makino) Nakai (eupolypod II), and constructed the phylogeny of ferns based on both 48 genes and 64 genes. The trees based on 48 genes and 64 genes are identical in topology, differing only in support values for four nodes, three of which showed higher support values for the 48‐gene dataset. Equisetum L. was resolved as the sister to the Psilotales–Ophioglossales clade, and Equisetales–Psilotales–Ophioglossales clade was sister to the clade of the leptosporangiate and marattioid ferns. The sister relationship between the tree fern clade and polypods was supported by 82% and 100% bootstrap values in the 64‐gene and 48‐gene trees, respectively. Within polypod ferns, Pteridaceae was sister to the clade of Dennstaedtiaceae and eupolypods with a high support value, and the relationship of Dennstaedtiaceae–eupolypods was strongly supported. With recent parallel advances in the phylogenetics of ferns using nuclear data, chloroplast phylogenomics shows great potential in providing a framework for testing the impact of reticulate evolution in the early evolution of ferns.
Sexual Concerns after (Pelvic) Radiotherapy: Is There Any Role for the Radiation Oncologist?
Authors: Krouwel Esmée M. , Nicolai Melianthe P. , van der Wielen Gerard J. , Putter Hein , Krol Augustinus D.G. , Pelger Rob C.M. , Incrocci Luca , Elzevier Henk Willem 
Published: September 2015

Abstract

Introduction
Sexual function is an important aspect of quality of life, and may be impaired after (pelvic) radiation.

Aim
The aim of this study was to identify practice, responsibility attitudes, knowledge, and barriers of Dutch radiation oncologists regarding sexual counseling.

Methods
A cross-sectional survey was performed using a 28-item questionnaire sent to all members of the Dutch Society for Radiotherapy and Oncology.

Main Outcome Measures
Self-reported practice, knowledge, barriers, need for training and responsibility attitudes in regard to demographic characteristics.

Results
Of the surveyed sample, 54.6% of the radiation oncologists completed the instrument (n = 119). Frequency of discussing sexual function was fluctuating, depending on the type of tumor. The majority of the responding radiation oncologists (75%) agreed that discussing sexual function is their responsibility, about one-third (33.6%) pointed at the involved specialist (surgeon, urologist, gynecologist, or oncologist), a fifth also considered the general practitioner responsible (21%). Additional training about discussing sexuality was required according to 44.4%, the majority agreed that sexual counseling should be a regular component of radiation oncology residency (n = 110, 94%). Barriers most mentioned included patient is too ill (36.2%), no angle or reason for asking (32.4%), advanced age of the patient (27%) and culture/religion (26.1%). For prostate cancer patients, phosphodiesterase 5 inhibitor information was supplied regularly (49.2%) and often (40.7%).

Conclusions
Radiation oncologists generally perform sexual counseling in case of pelvic radiation therapy, but not consistently in case of gastrointestinal, breast, and other cancers. The majority of radiation oncologists considered counseling on sexual functioning as a part of their job, some also pointed at the referring specialist or general practitioner. The findings suggest that awareness about sexual dysfunction is present among radiation oncologists, but responsibility for active counseling is uncertain. Results emphasize the need for providing educational and practical training, as well as a list for specialized referral.
Evaluation of I‐gel™ airway in different head and neck positions in anesthetized paralyzed children
Authors: Jain Divya , Ghai Babita , Bala Indu , Gandhi Komal , Banerjee Gargi 
Published: September 2015

SummaryBackgroundStudies that have compared and quantified the oropharynageal leak pressure (OPLP) and adequacy of ventilation with supraglottic airway devices in different head and neck positions have been done in adult populations. The effects of head–neck position changes on the functioning of I‐gel™ in pediatric population still remain unevaluated.AimThis study aimed to quantify the influence of different head and neck positions namely neutral, maximum flexion, and maximum extension on OPLP, ventilation scoring, and fiberoptic grading using I‐gel™ in anesthetized, paralyzed children.MethodsI‐gel™ was inserted in 30 paralyzed, anesthetized children scheduled for elective urological and orthopedic procedures. Anesthesia was induced with sevoflurane in oxygen. Atracurium was administered intravenously to facilitate neuromuscular relaxation. Recordings of OPLP in neutral, maximum flexion, and maximum extension were taken as primary outcome. Fiberoptic grading, insertion of ryle's tube and ventilation scoring were also measured in different head and neck positions as secondary outcomes.ResultsThe OPLP was significantly higher in flexion (27.6 ± 3.3 cm H2O, P = 0.000) and lower in extension (19.6 ± 3.2 cm H2O, P = 0.006) in comparison to the neutral position (23.2 ± 3.2 cm H2O). There was a worsening of the fiberoptic view in flexion compared to neutral position (0/5/19/6 vs 5/21/4/0). The ventilation score was poorer (1 [0–3], P < 0.05) and peak inspiratory pressures higher in flexion (15.2 ± 1.4 cm H2O, P = 0.000) compared to the neutral position (10.4 ± 1.6 cm H2O).ConclusionCaution is warranted in pediatric patients while ventilating with I‐gel™ in extreme flexion of head and neck owing to poor ventilation despite increase in OPLP.
Simultaneous expression of regulatory genes associated with specific drought‐adaptive traits improves drought adaptation in peanut
Authors: Ramu Vemanna S. , Swetha Thavarekere N. , Sheela Shekarappa H. , Babitha Chandrashekar K. , Rohini Sreevathsa , Reddy Malireddy K. , Tuteja Narendra , Reddy Chandrashekar P. , Prasad Trichi Ganesh , Udayakumar Makarla 
Published: September 2015

SummaryAdaptation of crops to drought‐prone rain‐fed conditions can be achieved by improving plant traits such as efficient water mining (by superior root characters) and cellular‐level tolerance mechanisms. Pyramiding these drought‐adaptive traits by simultaneous expression of genes regulating drought‐adaptive mechanisms has phenomenal relevance in improving stress tolerance. In this study, we provide evidence that peanut transgenic plants expressing Alfalfa zinc finger 1 (Alfin1), a root growth‐associated transcription factor gene, Pennisetum glaucum heat‐shock factor (PgHSF4) and Pea DNA helicase (PDH45) involved in protein turnover and protection showed improved tolerance, higher growth and productivity under drought stress conditions. Stable integration of all the transgenes was noticed in transgenic lines. The transgenic lines showed higher root growth, cooler crop canopy air temperature difference (less CCATD) and higher relative water content (RWC) under drought stress. Low proline levels in transgenic lines substantiate the maintenance of higher water status. The survival and recovery of transgenic lines was significantly higher under gradual moisture stress conditions with higher biomass. Transgenic lines also showed significant tolerance to ethrel‐induced senescence and methyl viologen‐induced oxidative stress. Several stress‐responsive genes such as heat‐shock proteins (HSPs), RING box protein‐1 (RBX1), Aldose reductase, late embryogenesis abundant‐5 (LEA5) and proline‐rich protein‐2 (PRP2), a gene involved in root growth, showed enhanced expression under stress in transgenic lines. Thus, the simultaneous expression of regulatory genes contributing for drought‐adaptive traits can improve crop adaptation and productivity under water‐limited conditions.
Excipients in Oral Antihistamines Can Perpetuate Allergic Contact Dermatitis
Authors: Tocci Elizabeth M. , Robinson Amanda , Belazarian Leah , Foley Elizabeth , Wiss Karen , Silvestri Dianne L. 
Published: September 2015

AbstractPropylene glycol is a well‐documented causative agent of allergic contact dermatitis (ACD). It is also reported to cause systemic dermatitis after ingestion of foods or medicines containing it and after intravenous injection of a medicine with propylene glycol in its base. We describe two adolescents with sensitivity to propylene glycol confirmed by patch testing whose dermatitis improved dramatically after cessation of oral antihistamines containing propylene glycol. We report these cases to alert providers to the potential for worsening of ACD due to systemic exposure to propylene glycol in patients with a cutaneous sensitivity to the allergen.
Endocrine Disruptors in Domestic Animal Reproduction: A Clinical Issue?
Authors: Magnusson Ulf , Persson Sara 
Published: September 2015

ContentsThe objective of this review was to discuss whether endocrine disruption is a clinical concern in domestic animal reproduction. To that end, we firstly summarize the phenomenon of endocrine disruption, giving examples of the agents of concern and their effects on the mammalian reproductive system. Then there is a brief overview of the literature on endocrine disruptors and domestic animal reproduction. Finally, the clinical implications of endocrine disruptors on the reproductive system of farm animals as well as in dogs and cats are discussed. It is concluded that the evidence for clinical cases of endocrine disruption by chemical pollutants is weak, whereas for phytooestrogens, it is well established. However, there is concern that particular dogs and cats may be exposed to man‐made endocrine disruptors.
Sperm Membrane Behaviour during Cooling and Cryopreservation
Authors: Sieme H , Oldenhof H , Wolkers WF 
Published: September 2015

ContentsNative sperm is only marginally stable after collection. Cryopreservation of semen facilitates transport and storage for later use in artificial reproduction technologies, but cryopreservation processing may result in cellular damage compromising sperm function. Membranes are thought to be the primary site of cryopreservation injury. Therefore, insights into the effects of cooling, ice formation and protective agents on sperm membranes may help to rationally design cryopreservation protocols. In this review, we describe membrane phase behaviour of sperm atsupra‐ and subzero temperatures. In addition, factors affecting membrane phase transitions and stability, sperm osmotic tolerance limits and mode of action of cryoprotective agents are discussed. It is shown how cooling only results in minor thermotropic non‐cooperative phase transitions, whereas freezing causes sharp lyotropic fluid‐to‐gel phase transitions. Membrane cholesterol content affects suprazero membrane phase behaviour and osmotic tolerance. The rate and extent of cellular dehydration coinciding with freezing‐induced membrane phase transitions are affected by the cooling rate and ice nucleation temperature and can be modulated by cryoprotective agents. Permeating agents such as glycerol can move across cellular membranes, whereas non‐permeating agents such as sucrose cannot. Both, permeating and non‐permeating protectants preserve biomolecular and cellular structures by forming a protective glassy state during freezing.
The interaction of genetic determinants in the outcome of HCV infection: evidence for discrete immunological pathways
Authors: Hydes T. J. , Moesker B. , Traherne J. A. , Ashraf S. , Alexander G. J. , Dimitrov B. D. , Woelk C. H. , Trowsdale J. , Khakoo S. I. 
Published: September 2015

AbstractDiversity within the innate and adaptive immune response to hepatitis C is important in determining spontaneous resolution (SR) and treatment response. The aim of this study was to analyze how these variables interact in combination; furthering our understanding of the mechanisms that drive successful immunological clearance. Multivariate analysis was performed on retrospectively collected data for 357 patients previously genotyped for interferon (IFN)‐λ3/4, killer cell immunoglobulin (KIR), human leukocyte antigen (HLA) class I and II and tapasin. High resolution KIR genotyping was performed for individuals with chronic infection and haplotypes determined. Outcomes for SR, IFN response and cirrhosis were examined. Statistical analysis included univariate methods, χ2 test for trend, multivariate logistic regression, synergy and principal component analysis (PCA). Although KIR2DL3:HLA‐C1C1 (P = 0.027), IFN‐λ3/4 rs12979860 CC (P = 0.027), tapasin G in individuals with aspartate at residue 114 of HLA‐B (TapG:HLA‐B114D) (P = 0.007) and HLA‐DRB1*04:01 (P = 0.014) were associated with SR with a strong additive influence (χ2 test for trend P < 0.0001); favorable polymorphisms did not interact synergistically, nor did patients cluster by outcome. In the treatment cohort, IFN‐λ3/4 rs12979860 CC was protective in hepatitis C virus (HCV) G1 infection and KIR2DL3:HLA‐C1 in HCV G2/3. In common with SR, variables did not interact synergistically. Polymorphisms predictive of viral clearance did not predict disease progression. In summary, different individuals resolve HCV infection using discrete and non‐interacting immunological pathways. These pathways are influenced by viral genotype. This work provides novel insights into the complexity of the interaction between host and viral factors in determining the outcome of HCV infection.
Successful Treatment of Carbapenemase-Producing Pandrug-Resistant Klebsiella pneumoniae Bacteremia
Authors: Camargo Jose F. , Simkins Jacques , Beduschi Thiago , Tekin Akin , Aragon Laura , Pérez-Cardona Armando , Prado Clara E. , Morris Michele I. , Abbo Lilian M. , Cantón Rafael 
Published: September 2015

ABSTRACT

New antibiotic options are urgently needed for the treatment of carbapenem-resistant
Enterobacteriaceae
infections. We report a 64-year-old female with prolonged hospitalization following an intestinal transplant who developed refractory bacteremia due to a serine carbapenemase-producing pandrug-resistant isolate of
Klebsiella pneumoniae
. After failing multiple antimicrobial regimens, the patient was successfully treated.
Draft Genome Sequence of the First Hypermucoviscous Klebsiella quasipneumoniae subsp.
quasipneumoniae
Isolate from a Bloodstream Infection
Authors: Arena Fabio , Henrici De Angelis Lucia , Pieralli Filippo , Di Pilato Vincenzo , Giani Tommaso , Torricelli Francesca , D’Andrea Marco Maria , Rossolini Gian Maria 
Published: September 2015

ABSTRACT

Klebsiella quasipneumoniae
is a recently described species, formerly identified as
K. pneumoniae
phylogroup KpII. Information on pathogenic and virulence potential of this species are lacking. We sequenced the genome of a hypermucoviscous
K. quasipneumoniae
clinical isolate showing a virulence genes content (
allABCDRS
,
kfuABC
, and
mrkABCDFHIJ
) peculiar to hypervirulent
K. pneumoniae
strains.
Draft Genome Sequences of Anaerolinea thermolimosa IMO-1, Bellilinea caldifistulae GOMI-1, Leptolinea tardivitalis YMTK-2, Levilinea saccharolytica KIBI-1, Longilinea arvoryzae KOME-1, Previously Described as Members of the Class
Anaerolineae
(
Chloroflexi
)
Authors: Matsuura Norihisa , Tourlousse Dieter M. , Ohashi Akiko , Hugenholtz Philip , Sekiguchi Yuji 
Published: September 2015

ABSTRACT

Members of the class
Anaerolineae
in the bacterial phylum
Chloroflexi
are widespread in a range of ecosystems but remain poorly understood. We present here the draft genome sequences of the type strains of five
Anaerolineae
species,
Anaerolinea thermolimosa
IMO-1,
Bellilinea caldifistulae
GOMI-1,
Leptolinea tardivitalis
YMTK-2,
Levilinea saccharolytica
KIBI-1, and
Longilinea arvoryzae
KOME-1.
Finished Genome Assembly of Yersinia pestis EV76D and KIM 10v
Authors: Johnson Shannon L. , Minogue Timothy D. , Daligault Hajnalka E. , Wolcott Mark J. , Teshima Hazuki , Coyne Susan R. , Davenport Karen W. , Jaissle James G. , Chain Patrick S. 
Published: September 2015

ABSTRACT

Here, we sequenced the completed genome of
Yersinia pestis
EV76D and KIM 10v, two genomes used as references in assay development, to improved high-quality draft status.
Genome Sequence of Brevibacillus reuszeri NRRL NRS-1206
T
, an
l
-
N
-Carbamoylase-Producing
Bacillus
-Like Bacterium
Authors: Wang Jie-ping , Liu Bo , Liu Guo-hong , Chen De-ju , Ge Ci-bin , Chen Zheng , Che Jian-mei 
Published: September 2015

ABSTRACT

Brevibacillus reuszeri
NRRL NRS-1206
T
is a Gram-positive, spore-forming, and strictly aerobic bacterium. Here, we report the draft 6.98-Mb genome sequence of
B. reuszeri
NRRL NRS-1206
T
, which is the first genome information of
B. reuszeri
and will provide useful information for genomic taxonomy and phylogenomics of
Bacillus
-like bacteria.
Genome Sequence Analysis of
Mycoplasma
sp
.
HU2014, Isolated from Tissue Culture
Authors: Calcutt Michael J. , Szikriszt Bernadett , Póti Ádám , Molnár János , Gervai Judit Z. , Tusnády Gábor E. , Foecking Mark F. , Szüts Dávid 
Published: September 2015

ABSTRACT

The draft genome sequence of a novel
Mycoplasma
strain, designated
Mycoplasma
sp. HU2014, has been determined. The genome comprises 1,084,927 nucleotides and was obtained from a mycoplasma-infected culture of chicken DT40 cells. Phylogenetic analysis places this taxon in a group comprising the closely related species
Mycoplasma yeatsii
and
Mycoplasma cottewii
.
HTLV-1 bZIP Factor RNA and Protein Impart Distinct Functions on T-cell Proliferation and Survival
Authors: Mitobe Yuichi , Yasunaga Jun-ichirou , Furuta Rie , Matsuoka Masao 
Published: September 2015

Abstract
Infection of T cells with human T-cell leukemia virus type-1 (HTLV-1) induces clonal proliferation and is closely associated with the onset of adult T-cell leukemia-lymphoma (ATL) and inflammatory diseases. Although Tax expression is frequently suppressed in HTLV-1-infected cells, the accessory gene, HTLV-1 bZIP factor (HBZ), is continuously expressed and has been implicated in HTLV-1 pathogenesis. Here, we report that transduction of mouse T cells with specific mutants of HBZ that distinguish between its RNA and protein activity results in differential effects on T-cell proliferation and survival. HBZ RNA increased cell number by attenuating apoptosis, whereas HBZ protein induced apoptosis. However, both HBZ RNA and protein promoted S-phase entry of T cells. We further identified that the first 50 bp of the HBZ coding sequence are required for RNA-mediated cell survival. Transcriptional profiling of T cells expressing wild-type HBZ, RNA, or protein revealed that HBZ RNA is associated with genes involved in cell cycle, proliferation, and survival, while HBZ protein is more closely related to immunological properties of T cells. Specifically, HBZ RNA enhances the promoter activity of survivin, an inhibitor of apoptosis, to upregulate its expression. Inhibition of survivin using YM155 resulted in impaired proliferation of several ATL cell lines as well as a T-cell line expressing HBZ RNA. The distinct functions of HBZ RNA and protein may have several implications for the development of strategies to control the proliferation and survival mechanisms associated with HTLV-1 infection and ATL. Cancer Res; 75(19); 4143–52. ©2015 AACR.
Contact Force in Atrial Fibrillation: Role of Atrial Rhythm and Ventricular Contractions
Authors: Sarkozy Andrea , Shah Dipen , Saenen Johan , Sieira Juan , Phlips Thomas , Boris Wim , Namdar Mehdi , Vrints Christiaan 
Published: September 2015

Background—
In an experimental model, variable and intermittent contact force (CF) resulted in a significant decrease in lesion volume. In humans, variability of CF during pulmonary vein isolation has not been characterized.

Methods and Results—

In 20 consecutive patients undergoing CF-guided circumferential pulmonary vein isolation, 914 radiofrequency applications (530 in sinus rhythm and 384 in atrial fibrillation) were analyzed. The variability of the 60% CF range (CF
60%
) was 17±9.6 g. Hundred seventy-one (19%) applications were delivered with constant, 717 (78%) with variable, and 26 (3%) with intermittent CF. The mean CF and force-time integral were significantly higher during applications with variable than with intermittent or constant CF. There was no significant difference in CF variability, CF
60%
variability, and force-time integral between applications delivered in sinus rhythm and atrial fibrillation. The main reasons for CF variability were systolo-diastolic heart movement (29%) and respiration (27%). In 10 additional patients, during adenosine-induced atrioventricular block, the minimum CF significantly increased at 19 sites (5.3±4.4 versus 13.4±5.9 g;
P
<0.001) and at 16 sites intermittent or variable CF became constant. At only 1 site systolo-diastolic movement remained the main reason for variable CF.

Conclusions—
CF during pulmonary vein isolation remains highly variable despite efforts to optimize contact. CF and CF parameters were similar during sinus rhythm and atrial fibrillation. The main reasons for CF variability are systolo-diastolic heart movement and respiration. The systolo-diastolic peaks and nadirs of CF are because of ventricular contractions at the large majority of pulmonary vein isolation sites.