bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensory optimization of gluten‐free hazelnut omelette and sugar‐modified chestnut pudding: A free choice profiling approach for enhanced traditional recipe formulations
Authors: Ortiz‐Solà J. , Almeida D. , López‐Mas L. , Kallas Z. , Abadias M. , Barros L. , Martín‐Gómez H. , Aguiló‐Aguayo I. 
Published: August 2024

AbstractThe Mediterranean region is distinguished by its gastronomic diversity and a wide variety of indigenous nut crops. In line with changing global food consumers’ preferences, a noteworthy aspect is the increasing demand to the use of local varieties in recipe formulation. The aim of the present study was to incorporate the Terra Fria chestnut (Portugal) and Negreta hazelnut from Reus (Spain) in traditional Mediterranean recipes. The sensory, technofunctional, nutritional, and shelf‐life characterization were investigated in hazelnut omelette (gluten and gluten‐free) and chestnut pudding (sugar and sugar‐free) formulations. Results conducted by trained assessors using the free choice profiling (FCP) showed that hazelnut omelette samples were described as “creamy,” “smooth,” and “handmade.” In addition, the texture obtained with the hazelnut omelette gluten‐free version showed the softest textural profile analysis attributes, with lower values for hardness (2.43 ± 0.36 N), adhesiveness (−0.38 ± 0.00 g s) and gumminess (2.12 ± 0.14). Furthermore, the shelf‐life studies revealed a more golden color (>14.43 of a* CIELAB coordinate) and a lower moisture content (25.36%–43.59%) in the hazelnut flour formulation, in addition to the enrichment in terms of protein (8.36 g/100 g), fiber, and healthy fats. In the case of chestnut pudding, it was observed that the study parameters did not differ significantly from its sweetened analogue with positive attributes in FCP (“toasted,” “fluffy,” and “sweet”), positioning it as a viable alternative to sugar in this application. Therefore, both hazelnut flour in hazelnut omelette and oligofructose in chestnut pudding proved to be promising ingredients in the formulation of gluten‐free and sugar‐free developed products, offering attractive organoleptic and textural characteristics.
Characterization, stability, and curcumin bioaccessibility of buckwheat flower polysaccharide conjugate emulsion
Authors: Zhang Yu , Xu Yanghui , Fang Tianqi , Qiu Qing , Chen Maobin 
Published: August 2024

AbstractIn this study, buckwheat flower polysaccharide conjugates (BFPCs) were synthesized and evaluated for their emulsification properties. The stability of BFPC‐stabilized emulsions was assessed through particle size analysis, zeta potential measurements, microscopic observations, and rheological tests. Gum Arabic served as a control to compare BFPC's emulsifying efficacy across varying storage conditions, including exposure to metal ions, pH variations, and different heat treatment temperatures. Results showed that BFPC significantly lowered interfacial tension (16.2 mN/m) and effectively stabilized emulsions containing 60 wt% medium‐chain triglycerides at a concentration of 1.0 wt%. Over a 20‐day storage period, BFPC emulsions demonstrated robust resistance to heat (60–90°C), acidic conditions (pH 2.0–9.0), and ion concentrations (Na+, Ca2+). Moreover, in a high oil phase emulsion, BFPC enhanced the bioavailability of curcumin to 27.05%, markedly higher than the 7.10% observed without emulsification, underscoring its potential in nutrient delivery applications.Practical ApplicationDue to its excellent resistance, long‐time emulsifying stability under different conditions, and its good effect in curcumin embedding, BFPC has a broad prospect and can be widely used under various conditions in food industry.
Enhancing metabarcoding efficiency and ecological insights through integrated taxonomy and DNA reference barcoding: A case study on beach meiofauna
Authors: Macher Jan‐Niklas , Martínez Alejandro , Çakir Sude , Cholley Pierre‐Etienne , Christoforou Eleni , Curini Galletti Marco , van Galen Lotte , García‐Cobo Marta , Jondelius Ulf , de Jong Daphne , Leasi Francesca , Lemke Michael , Rubio Lopez Iñigo , Sánchez Nuria , Sørensen Martin Vinther , Todaro M. Antonio , Renema Willem , Fontaneto Diego 
Published: August 2024

AbstractMolecular techniques like metabarcoding, while promising for exploring diversity of communities, are often impeded by the lack of reference DNA sequences available for taxonomic annotation. Our study explores the benefits of combining targeted DNA barcoding and morphological taxonomy to improve metabarcoding efficiency, using beach meiofauna as a case study. Beaches are globally important ecosystems and are inhabited by meiofauna, microscopic animals living in the interstitial space between the sand grains, which play a key role in coastal biodiversity and ecosystem dynamics. However, research on meiofauna faces challenges due to limited taxonomic expertise and sparse sampling. We generated 775 new cytochrome c oxidase I DNA barcodes from meiofauna specimens collected along the Netherlands' west coast and combined them with the NCBI GenBank database. We analysed alpha and beta diversity in 561 metabarcoding samples from 24 North Sea beaches, a region extensively studied for meiofauna, using both the enriched reference database and the NCBI database without the additional reference barcodes. Our results show a 2.5‐fold increase in sequence annotation and a doubling of species‐level Operational Taxonomic Units (OTUs) identification when annotating the metabarcoding data with the enhanced database. Additionally, our analyses revealed a bell‐shaped curve of OTU richness across the intertidal zone, aligning more closely with morphological analysis patterns, and more defined community dissimilarity patterns between supralittoral and intertidal sites. Our research highlights the importance of expanding molecular reference databases and combining morphological taxonomy with molecular techniques for biodiversity assessments, ultimately improving our understanding of coastal ecosystems.
Failure of isoxsuprine HCl to increase sheep fertility after cervical versus laparoscopic AI using chilled semen at different durations
Authors: Bülbül Bülent , Dayanıklı Cemal , Şengül Ebru , Turan Aydın , Aksoy Muhammet , Nur Zekariya 
Published: August 2024

AbstractTo improve the fertility of cervical artificial insemination (AI) in sheep, we investigated isoxsuprine HCl usage on the cervical passage during cervical AI. We also compared cervical and laparoscopic AI fertility results of using chilled semen at different durations. Semen was collected from rams and diluted as 20 × 106 or 400 × 106 spermatozoa/straw for laparoscopic and cervical AI, respectively, and chilled to 4°C within 2 h. Sheep were inseminated with chilled semen for 8 or 24 h via the laparoscopic or cervical AI method. Moreover, some of the cervical inseminated sheep were injected intramuscularly with 0.5 mg/kg of isoxsuprine HCl 15 min before AI. As a result, the use of isoxsuprine HCl did not affect cervical transit and fertility. In addition, fertility was affected by the storage duration of the semen; laparoscopic AI was more successful than cervical AI in terms of fertility; if cervical AI is performed, the duration between semen collection and AI should be less than 8 h after chilling the semen at 4°C, and if laparoscopic AI is performed, the time between semen collection and insemination can be up to 24 h after chilling the semen at 4°C. Longer storage periods should be studied.
Targeting the redox vulnerability of acute myeloid leukaemia cells with a combination of auranofin and vitamin C
Authors: Hei Zhiliang , Yang Shujun , Ouyang Guifang , Hanna Jolimar , Lepoivre Michel , Huynh Tony , Aguinaga Lorea , Cassinat Bruno , Maslah Nabih , Bourge Mickaël , Golinelli‐Cohen Marie‐Pierre , Guittet Olivier , Vallières Cindy , Vernis Laurence , Fenaux Pierre , Huang Meng‐Er 
Published: August 2024

SummaryAcute myeloid leukaemia (AML) is a heterogeneous disease characterized by complex molecular and cytogenetic abnormalities. Pro‐oxidant cellular redox status is a common hallmark of AML cells, providing a rationale for redox‐based anticancer strategy. We previously discovered that auranofin (AUF), initially used for the treatment of rheumatoid arthritis and repositioned for its anticancer activity, can synergize with a pharmacological concentration of vitamin C (VC) against breast cancer cell line models. In this study, we observed that this drug combination synergistically and efficiently killed cells of leukaemic cell lines established from different myeloid subtypes. In addition to an induced elevation of reactive oxygen species and ATP depletion, a rapid dephosphorylation of 4E‐BP1 and p70S6K, together with a strong inhibition of protein synthesis were early events in response to AUF/VC treatment, suggesting their implication in AUF/VC‐induced cytotoxicity. Importantly, a study on 22 primary AML specimens from various AML subtypes showed that AUF/VC combinations at pharmacologically achievable concentrations were effective to eradicate primary leukaemic CD34+ cells from the majority of these samples, while being less toxic to normal cord blood CD34+ cells. Our findings indicate that targeting the redox vulnerability of AML with AUF/VC combinations could present a potential anti‐AML therapeutic approach.
Comprehensive macro and micro views on immune cells in ischemic heart disease
Authors: Zhao Yongjian , Tan Mingyue , Yin Yunfei , Zhang Jun , Song Yiyi , Li Hang , Yan Lin , Jin Yifeng , Wu Ziyue , Yang Tianke , Jiang Tingbo , Li Hongxia 
Published: August 2024

AbstractIschemic heart disease (IHD) is a prevalent cardiovascular condition that remains the primary cause of death due to its adverse ventricular remodelling and pathological changes in end‐stage heart failure. As a complex pathologic condition, it involves intricate regulatory processes at the cellular and molecular levels. The immune system and cardiovascular system are closely interconnected, with immune cells playing a crucial role in maintaining cardiac health and influencing disease progression. Consequently, alterations in the cardiac microenvironment are influenced and controlled by various immune cells, such as macrophages, neutrophils, dendritic cells, eosinophils, and T‐lymphocytes, along with the cytokines they produce. Furthermore, studies have revealed that Gata6+ pericardial cavity macrophages play a key role in regulating immune cell migration and subsequent myocardial tissue repair post IHD onset. This review outlines the role of immune cells in orchestrating inflammatory responses and facilitating myocardial repair following IHD, considering both macro and micro views. It also discusses innovative immune cell‐based therapeutic strategies, offering new insights for further research on the pathophysiology of ischemic heart disease and immune cell‐targeted therapy for IHD.
LCP‐Tacrolimus Extended‐Release (Envarsus XR) Use in Adolescent and Young Adult Solid Organ Transplant Recipients
Authors: Bae Esther K. , Chandran Mary Moss , Everitt Melanie D. , Benz Eric , Bock Margret 
Published: August 2024

ABSTRACTIntroductionLimited published experience describes once daily, extended‐release tacrolimus (LCP‐Tac) use in pediatric solid organ transplantation (SOT), particularly nonrenal SOT. LCP‐Tac can simplify immunosuppression (IS) regimens, minimize immediate release‐tacrolimus (IR‐Tac)‐associated adverse effects, and promote adherence. This study describes the successful use of LCP‐Tac in adolescent and young adult (AYA) SOT populations.MethodsA single‐center, retrospective chart review of AYA SOT recipients (age < 25 years) converted from IR‐Tac to LCP‐Tac. Graft survival, biopsy‐proven acute rejection (BPAR), infection rates, estimated glomerular filtration rate (eGFR), and pill burden were assessed at five time points postconversion (1, 3, 6, 12, and 24 months). Intrapatient variability of tacrolimus, as assessed by coefficient of variability (CV%), was also analyzed.ResultsTwenty‐nine AYA SOT recipients (19 heart, 6 kidney, and 4 liver) were converted to LCP‐Tac, with a median age of 17.4 years at conversion. Conversion, mainly due to perceived or identified medication nonadherence, occurred at a median of 5.4 years posttransplant. No graft loss occurred within 24 months of conversion, and BPAR incidence rate was consistent with previous reports for these populations. Only one patient experienced CMV infection. Renal function remained stable postconversion.ConclusionSuccessful conversion from IR‐Tac to LCP‐Tac was demonstrated in AYA heart, kidney, and liver transplant recipients. These AYA SOT recipients experienced reduced pill burden and improved tacrolimus trough concentration variability. However, the impact on medication adherence warrants further investigation. Future research should explore the targeted use of LCP‐Tac to enhance IS tolerability and medication adherence in young SOT populations.
Comparison of R‐CHOP‐14 and R‐mini‐CHOP in older adults with diffuse large B‐cell lymphoma—A retrospective multicenter cohort study
Authors: Dilbaz Zelal Guel , Denker Sophy , Ankermann Carla , Bittenbring Joerg‐Thomas , Kaddu‐Mulindwa Dominic , Kunte Ameya S. , Hünecke Sascha , Poeschel Viola , Stilgenbauer Stephan , Thurner Lorenz , Na Il‐Kang , Bewarder Moritz , Christofyllakis Konstantinos 
Published: August 2024

AbstractDiffuse large B‐cell lymphoma (DLBCL) is the most common lymphoma entity, and its incidence increases with age. There is a paucity of data regarding use of biweekly R‐CHOP (R‐CHOP‐14) in patients ≥80 years of age. We performed a retrospective cohort study of patients with DLBCL aged ≥80 years treated with R‐CHOP‐14 and R‐miniCHOP in two academic tertiary centers in Germany between 01/01/2005 and 12/30/2019. Overall, 79 patients were included. Median age was 84 years (range 80–91). Despite higher CR rates with R‐CHOP‐14 (71.4% vs. 52.4%), no statistically significant difference could be found between patients treated with R‐CHOP‐14 and R‐miniCHOP regarding overall survival (OS) (p = .88, HR 0.94, 95% CI = 0.47–1.90) and progression‐free survival (PFS) (p = .26, HR 0.66, 95% CI = 0.32–1.36). At a median follow‐up of 40 months, the 2‐year OS rates were 56% with R‐CHOP‐14 and 53% with R‐miniCHOP. Two‐year PFS rates were 46% for R‐CHOP‐14 and 50% for R‐mini‐CHOP. Relative dose intensity of chemotherapy did not correlate with OS (p = .72). With the caveat of a retrospective cohort study, we conclude that lacking a difference in OS, R‐miniCHOP should be preferred for most patients with untreated DLBCL aged ≥80 years.
Heterogeneous spatial tuning in the auditory pathway of the Mongolian Gerbil (Meriones unguiculatus)
Authors: van den Wildenberg Maury F. , Bremen Peter 
Published: August 2024

AbstractSound‐source localization is based on spatial cues arising due to interactions of sound waves with the torso, head and ears. Here, we evaluated neural responses to free‐field sound sources in the central nucleus of the inferior colliculus (CIC), the medial geniculate body (MGB) and the primary auditory cortex (A1) of Mongolian gerbils.Using silicon probes we recorded from anaesthetized gerbils positioned in the centre of a sound‐attenuating, anechoic chamber. We measured rate‐azimuth functions (RAFs) with broad‐band noise of varying levels presented from loudspeakers spanning 210° in azimuth and characterized RAFs by calculating spatial centroids, Equivalent Rectangular Receptive Fields (ERRFs), steepest slope locations and spatial‐separation thresholds. To compare neuronal responses with behavioural discrimination thresholds from the literature we performed a neurometric analysis based on signal‐detection theory.All structures demonstrated heterogeneous spatial tuning with a clear dominance of contralateral tuning. However, the relative amount of contralateral tuning decreased from the CIC to A1. In all three structures spatial tuning broadened with increasing sound‐level. This effect was strongest in CIC and weakest in A1. Neurometric spatial‐separation thresholds compared well with behavioural discrimination thresholds for locations directly in front of the animal.Our findings contrast with those reported for another rodent, the rat, which exhibits homogenous and sharply delimited contralateral spatial tuning. Spatial tuning in gerbils resembles more closely the tuning reported in A1 of cats, ferrets and non‐human primates. Interestingly, gerbils, in contrast to rats, share good low‐frequency hearing with carnivores and non‐human primates, which may account for the observed spatial tuning properties.
Analysis of individual alpha frequency in a large cohort from a tertiary memory center
Authors: Cecchetti Giordano , Agosta Federica , Canu Elisa , Basaia Silvia , Rugarli Giulia , Curti Davide G. , Coraglia Federico , Cursi Marco , Spinelli Edoardo G. , Santangelo Roberto , Caso Francesca , Fanelli Giovanna Franca , Magnani Giuseppe , Filippi Massimo 
Published: August 2024

AbstractBackground and PurposePrecise and timely diagnosis is crucial for the optimal use of emerging disease‐modifying treatments for Alzheimer disease (AD). Electroencephalography (EEG), which is noninvasive and cost‐effective, can capture neural abnormalities linked to various dementias. This study explores the use of individual alpha frequency (IAF) derived from EEG as a diagnostic and prognostic tool in cognitively impaired patients.MethodsThis retrospective study included 375 patients from the tertiary Memory Clinic of IRCCS San Raffaele Hospital, Milan, Italy. Participants underwent clinical and neuropsychological assessments, brain imaging, cerebrospinal fluid biomarker analysis, and resting‐state EEG. Patients were categorized by amyloid status, the AT(N) classification system, clinical diagnosis, and mild cognitive impairment (MCI) progression to AD dementia. IAF was calculated and compared among study groups. Receiver operating characteristic (ROC) analysis was used to calculate its discriminative performance.ResultsIAF was higher in amyloid‐negative subjects and varied significantly across AT(N) groups. ROC analysis confirmed IAF's ability to distinguish A–T–N– from the A+T+N+ and A+T–N+ groups. IAF was lower in AD and Lewy body dementia patients compared to MCI and other dementia types, with moderate discriminatory capability. Among A+ MCI patients, IAF was significantly lower in those who converted to AD within 2 years compared to stable MCI patients and predicted time to conversion (p < 0.001, R = 0.38).ConclusionsIAF is a valuable tool for dementia diagnosis and prognosis, correlating with amyloid status and neurodegeneration. It effectively predicts MCI progression to AD, supporting its use in early, targeted interventions in the context of disease‐modifying treatments.
Climate change effects on abundance and distribution of the European eel in Türkiye
Authors: Mestav Burcu , Özdilek Şükran Yalçın , Acar Zahide , Gökkaya Kemal , Partal Nurbanu 
Published: August 2024

AbstractSpatial and temporal distribution of European eel (Anguilla anguilla) during 1967–2020 was used to model European eel distribution in response to climate variability. Modeling integrated predictions from two distinct models for inferences. First, modeling of the relationship between temperature and precipitation and European eel catch values using multiple time‐series regression analyses showed that climate variables contributed to eel distribution in Türkiye. Eel catch became restricted to western and southern coasts of Türkiye and total catch decreased between 1967 and 2020. From 1967 to 2020, favorable climatic conditions for European eels in Türkiye were characterized by moderate temperatures. Furthermore, projections for 2050 and 2070 suggested that suitable habitat would be lost in Türkiye. Our findings underscore the urgent need for immediate and widespread implementation of effective conservation policies to mitigate threats to European eel in Türkiye and globally.
Connectivity and climate influence diversity–stability relationships across spatial scales in European butterfly metacommunities
Authors: Alves Wagner de F. , de Souza Leonardo C. , Schweiger Oliver , di Cavalcanti Victor R. , Settele Josef , Wiemers Martin , Schmucki Reto , Kuussaari Mikko , Tzortzakaki Olga , Pettersson Lars B. , Fontaine Benoît , van Swaay Chris , Stefanescu Constantí , Maes Dirk , WallisDeVries Michiel F. , Gianuca Andros T. 
Published: August 2024

AbstractAimAnthropogenic‐driven biodiversity loss can impact ecosystem stability. However, most studies have only evaluated the diversity–stability relationship at the local scale and we do not fully understand which factors stabilize animal populations and communities across scales. Here, we investigate the role of species dispersal ability, climate, spatial distance and different facets of biodiversity on the stability of butterfly populations and communities across multiple spatial scales.LocationPrimarily Western Europe.Time Period2005–2016.Major Taxa StudiedButterflies (Rhopalocera) of Europe.MethodsWe assembled a continent‐wide database of European butterflies' abundance and used Structural Equation Modelling to evaluate the direct and indirect effects of multiple stabilizing mechanisms. In parallel, we tested the effect of dispersal ability on the stability at multiple spatial scales, using a butterfly mobility index as an indicator of dispersal capacity.ResultsRegional stability strongly reflected local stability, which in turn was driven by both taxonomic and functional α‐diversity. Spatial asynchrony was also important for regional stability and it was driven by both functional β‐diversity and metapopulation asynchrony, which in turn increased with spatial distance among communities. We observed a positive effect of temperature on functional α‐diversity and on local stability, whereas precipitation negatively influenced local diversity. Finally, spatial asynchrony contributed more to the regional stability of less mobile species compared to highly mobile ones, indicating that both extrinsic and intrinsic determinants of connectivity impact regional stability indirectly.Main ConclusionsOur results demonstrate the importance of local and regional processes for regional stability. However, the relative contribution of spatial asynchrony and metapopulation asynchrony increases with connectivity loss, especially for less mobile species, indicating that landscape management should be tailored depending on the dispersal capacity of organisms. Both local biodiversity loss and regional biotic homogenization destabilize metacommunities, with potential implications for the reliable provision of ecosystem functions.
Use it or lose it: The problem of labour underutilization among immigrant workers in Canada
Authors: Banerjee Rupa , Lamb Danielle , Lam Laura 
Published: August 2024

AbstractCanada is widely recognized as a desirable destination for new immigrants and all levels of governments are generally supportive of ambitious immigration targets set to help meet labour demand. Canada's immigration system is based primarily on human capital, selecting the world's most highly skilled newcomers. However, immigrants to Canada have often faced difficulty in attaining labour market outcomes commensurate with their knowledge and experience. In this analysis, we examine the paradox apparent in the Canadian immigration system—the selection criteria attract highly educated and skilled workers, yet many are not able to find employment opportunities that match their abilities—through the lens of the Labour Utilization Framework. Using data from the Canadian Labour Force Survey for the years 2006–2019 inclusive, we explore five different dimensions of skill underutilization or brain waste: involuntary part‐time work, minimum wage work, unemployment, over‐education (i.e., underemployment), and worker discouragement. Our results suggest that on all dimensions of labour underutilization measured in the study, immigrants are overwhelmingly at a disadvantage relative to their Canadian‐born, non‐Indigenous counterparts. We discuss the ethical implications of immigrant brain waste for both individuals and society and conclude by suggesting some possible policy responses to improve the utilization of immigrant talent in Canada.