bluuebunny/crossref_metadata_2025_split huggingface dataset:
Society for Pediatric Psychology Award for Outstanding Mentorship: Mentoring: An Early Kansas Model
Authors: Christophersen Edward R. 
Published: December 2014

The most rewarding aspect of mentoring is sharing your insights and knowledge with the future leaders in the field. The objective of this article is to share the most important lessons I have learned from my own mentors and from decades of mentoring some of the best and brightest students in pediatric psychology. Young professionals in the field need to know their medical subspecialty almost as comprehensively as they know psychology. Find out what the patients and practitioners need, and adapt your training to be effective in the medical setting. Identify your professional goals and archive your contributions regularly to assist you in advancing your career. Find a mentor who has the skills, experiences, and connections you desire for your own future. Then pay it forward and mentor the next generation of leaders who will continue to expand and improve the field of pediatric psychology.
Dynamically controlling the emission of single excitons in photonic crystal cavities
Authors: Pagliano Francesco , Cho YongJin , Xia Tian , van Otten Frank , Johne Robert , Fiore Andrea 
Published: December 2014

AbstractSingle excitons in semiconductor microcavities represent a solid state and scalable platform for cavity quantum electrodynamics, potentially enabling an interface between flying (photon) and static (exciton) quantum bits in future quantum networks. While both single-photon emission and the strong coupling regime have been demonstrated, further progress has been hampered by the inability to control the coherent evolution of the cavity quantum electrodynamics system in real time, as needed to produce and harness charge–photon entanglement. Here using the ultrafast electrical tuning of the exciton energy in a photonic crystal diode, we demonstrate the dynamic control of the coupling of a single exciton to a photonic crystal cavity mode on a sub-nanosecond timescale, faster than the natural lifetime of the exciton. This opens the way to the control of single-photon waveforms, as needed for quantum interfaces, and to the real-time control of solid-state cavity quantum electrodynamics systems.
A tetrahelical DNA fold adopted by tandem repeats of alternating GGG and GCG tracts
Authors: Kocman Vojč , Plavec Janez 
Published: December 2014

AbstractDNA can form diverse higher-order structures, whose details are greatly dependent on nucleotide sequence. G-rich sequences containing four or more repeats of three guanines are expected to form G-quadruplexes. Here we show that DNA sequences with GGGAGCG repeats found in the regulatory region of the PLEKHG3 gene are capable of forming tetrahelical DNA structures that are distinct from G-quadruplexes. The d(GGGAGCGAGGGAGCG) sequence, VK1, forms a dimer. Two VK1 sequences connected by an adenine residue, VK2, fold into a monomer, which shares identical structural characteristics with the VK1 fold. Their four-stranded architectures are stabilized by four G-C, four G-A and six G-G base pairs. No G-quartets or Hoogsteen-type hydrogen-bonded guanine residues are present and the overall topology is conserved in the presence of Li+, Na+, K+ and NH4+ ions. Unique structural features include two edgewise loops on each side of the structure stabilized by three G-G base pairs in N1-carbonyl symmetric geometry.
The regions within the N-terminus critical for human glucagon like peptide-1 receptor (hGLP-1R) cell Surface expression
Authors: Thompson Aiysha , Kanamarlapudi Venkateswarlu 
Published: December 2014

AbstractThe hGLP-1R is a target for the treatment of type 2 diabetes and belongs to the class B family of GPCRs. Like other class B GPCRs, the GLP-1R contains an N-terminal signal peptide (SP) and undergoes N-linked glycosylation, which are important for its trafficking and maturation. This study analysed the role of the SP, the hydrophobic region after the SP (HRASP), glycosylation and the conserved residues within the N-terminus in GLP-1R trafficking. HGLP-1R targeted to the cell surface showed no SP and the SP deleted mutant, but not the mutants defective in SP cleavage, showed cell surface expression, demonstrating the importance of SP cleavage for hGLP-1R cell surface expression. The N-terminal deletions of hGLP-1R revealed that the HRASP, not the SP, is essential for cell surface expression of GLP-1R. Further, inhibition of hGLP-1R glycosylation prevented cell surface expression of the receptor. Mutation of Trp39, Tyr69 and Tyr88, which are required for agonist binding, in the GLP-1R abolished cell surface expression of the receptor independent of the SP cleavage or N-linked glycosylation. In conclusion, the N-terminus of hGLP-1R regulates receptor trafficking and maturation. Therefore this study provides insight into the role of hGLP-1R N-terminus on the receptor cell surface expression.
Digital Quantum Rabi and Dicke Models in Superconducting Circuits
Authors: Mezzacapo A. , Las Heras U. , Pedernales J. S. , DiCarlo L. , Solano E. , Lamata L. 
Published: December 2014

AbstractWe propose the analog-digital quantum simulation of the quantum Rabi and Dicke models using circuit quantum electrodynamics (QED). We find that all physical regimes, in particular those which are impossible to realize in typical cavity QED setups, can be simulated via unitary decomposition into digital steps. Furthermore, we show the emergence of the Dirac equation dynamics from the quantum Rabi model when the mode frequency vanishes. Finally, we analyze the feasibility of this proposal under realistic superconducting circuit scenarios.
Arsenolipids in oil from blue whiting Micromesistius poutassou – evidence for arsenic-containing esters
Authors: Taleshi Mojtaba S. , Raber Georg , Edmonds John S. , Jensen Kenneth B. , Francesconi Kevin A. 
Published: December 2014

AbstractArsenic-containing lipids in the oil from the blue whiting fish (Micromesistius poutassou) were separated into three broad polarity groups and investigated by HPLC and mass spectrometry. A total of 11 arsenolipids including 4 new compounds were identified. The polar lipid fraction constituting 24% of the total arsenolipid content (which totalled 2.16 μg As/g) contained four known dimethylarsinoyl fatty acids and three known dimethylarsinoyl hydrocarbons. The less polar fraction (ca 30% of the total arsenolipids) contained four new dimethylarsinoyl hydrocarbons with chain lengths 22–30 carbons, in addition to more complex arsenicals that hydrolysed to known dimethylarsinoyl fatty acids suggesting they were conjugated carboxylic acids, presumably esters. The rest of the lipid-soluble arsenic (ca 45% of the total) remained in the non-polar fraction together with the bulk of the fish oil lipids, a complex mixture of compounds that precluded identification of the small amounts of arsenolipids.
Observed bromodomain flexibility reveals histone peptide- and small molecule ligand-compatible forms of ATAD2
Authors: Poncet-Montange Guillaume , Zhan Yanai , Bardenhagen Jennifer P. , Petrocchi Alessia , Leo Elisabetta , Shi Xi , Lee Gilbert R. , Leonard Paul G. , Geck Do Mary K. , Cardozo Mario G. , Andersen Jannik N. , Palmer Wylie S. , Jones Philip , Ladbury John E. 
Published: December 2014

Preventing histone recognition by bromodomains emerges as an attractive therapeutic approach in cancer. Overexpression of ATAD2 (ATPase family AAA domain-containing 2 isoform A) in cancer cells is associated with poor prognosis making the bromodomain of ATAD2 a promising epigenetic therapeutic target. In the development of an in vitro assay and identification of small molecule ligands, we conducted structure-guided studies which revealed a conformationally flexible ATAD2 bromodomain. Structural studies on apo–, peptide–and small molecule–ATAD2 complexes (by co-crystallization) revealed that the bromodomain adopts a ‘closed’, histone-compatible conformation and a more ‘open’ ligand-compatible conformation of the binding site respectively. An unexpected conformational change of the conserved asparagine residue plays an important role in driving the peptide-binding conformation remodelling. We also identified dimethylisoxazole-containing ligands as ATAD2 binders which aided in the validation of the in vitro screen and in the analysis of these conformational studies.
Generation of suppressive blood cells for control of allograft rejection
Authors: Kleist Christian , Sandra-Petrescu Flavius , Jiga Lucian , Dittmar Laura , Mohr Elisabeth , Greil Johann , Mier Walter , Becker Luis E. , Lang Peter , Opelz Gerhard , Terness Peter 
Published: December 2014

Our previous studies in rats showed that incubation of monocytic dendritic cells (DCs) with the chemotherapeutic drug mitomycin C (MMC) renders the cells immunosuppressive. Donor-derived MMC–DCs injected into the recipient prior to transplantation prolonged heart allograft survival. Although the generation of DCs is labour-intensive and time-consuming, peripheral blood mononuclear cells (PBMCs) can be easily harvested. In the present study, we analyse under which conditions DCs can be replaced by PBMCs and examine their mode of action. When injected into rats, MMC-incubated donor PBMCs (MICs) strongly prolonged heart allograft survival. Removal of monocytes from PBMCs completely abrogated their suppressive effect, indicating that monocytes are the active cell population. Suppression of rejection was donor-specific. The injected MICs migrated into peripheral lymphoid organs and led to an increased number of regulatory T-cells (Tregs) expressing cluster of differentiation (CD) markers CD4 and CD25 and forkhead box protein 3 (FoxP3). Tolerance could be transferred to syngeneic recipients with blood or spleen cells. Depletion of Tregs from tolerogenic cells abrogated their suppressive effect, arguing for mediation of immunosuppression by CD4+CD25+FoxP3+ Tregs. Donor-derived MICs also prolonged kidney allograft survival in pigs. MICs generated from donor monocytes were applied for the first time in humans in a patient suffering from therapy-resistant rejection of a haploidentical stem cell transplant. We describe, in the present paper, a simple method for in vitro generation of suppressor blood cells for potential use in clinical organ transplantation. Although the case report does not allow us to draw any conclusion about their therapeutic effectiveness, it shows that MICs can be easily generated and applied in humans.
Registration artifacts in international comparisons of infant mortality
Authors: Kramer Michael S. , Platt Robert W. , Yang Hong , Haglund Bengt , Cnattingius Sven , Bergsjo Per 
Published: December 2014

SummaryLarge differences in infant mortality are reported among and within industrialised countries. We hypothesised that these differences are at least partly the result of intercountry differences in registration of infants near the borderline of viability (<750 g birthweight) and/or their classification as stillbirths vs. live births. We used the database of the International Collaborative Effort (ICE) on Perinatal and Infant Mortality to compare infant mortality rates and registration practices in Norway (n = 112 484), Sweden (n = 215 908), Israeli Jews (n
= 148 123), Israeli non‐Jews (n = 52 606), US Whites (n = 6 074 222)
and US Blacks (n = 1 328 332). To avoid confounding by strong secular trends in these outcomes, we restricted our analysis to 1987–88, the most recent years for which data are available in the ICE database for all six groups. Compared with Norway (with an infant mortality rate of 8.5 per 1000), the crude relative risks [95% confidence intervals] were 0.75 [0.69,0.81] in Sweden, 0.97 [0.90,1.06] in Israeli Jews, 1.98 [1.81,2.17] in Israeli non‐Jews, 0.95 [0.89,1.01] in US Whites and 2.05 [1.95,2.19] in US Blacks. For borderline‐viable infants, fetal deaths varied twofold as a proportion of perinatal deaths, with Norway reporting the highest (83.9% for births <500 g and 61.8% for births 500–749 g) and US Blacks the lowest (40.3% and 37.6% respectively) proportions. Reported proportions of live births <500 g varied 50‐fold from 0.6 and 0.7 per 10 000 in Sweden and Israeli Jews and non‐Jews to 9.1 and 33.8 per 10 000 in US Whites and Blacks respectively. Reported proportions 500–749 g varied sevenfold from 7.5 per 10 000 in Sweden to 16.2 and 55.4 in US Whites and Blacks respectively. After eliminating births <750 g, the relative risks (again with Norway as the reference) of infant mortality changed drastically for US Whites and Blacks: 0.82 [0.76,0.87] and 1.42 [1.33,1.53] respectively. The huge disparities in the ratio of fetal to infant deaths <750 g and in the proportion of live births <750 g among these developed countries probably result from differences in birth and death registration practices. International comparisons and rankings of infant mortality should be interpreted with caution.
Measurement of enhanced backscattering from randomly distributed conductive spheres with concentrically different number densities at 60 GHz
Authors: Kaneko K. , Ihara T. 
Published: December 2014

The effective utilisation of millimetre‐wave radar for precipitation measurements seems to require quantitative evaluation methods of enhanced backscattering from randomly distributed particles. One such method is a computer simulation technique proposed in the literature [Radio Science, 2006, 41, RS6002], a technique that takes into account the spherical wavefront and directivity functions of transmitting and receiving antennas. The effectiveness of this technique has been previously verified by the present authors by comparing laboratory‐controlled scattering experiments performed at 60 GHz using cylindrical scattering volumes, where conductive spheres of a monodispersive size are randomly distributed with statistically uniform number densities. In this latest reported work, the effectiveness of this computer simulation technique is further demonstrated for the case where conductive spheres are randomly and non‐uniformly distributed with concentrically different number densities.
Design of microstrip lowpass filter with wide stopband and sharp roll‐off using hexangular shaped resonator
Authors: Hayati M. , Gholami M. , Vaziri H.S. , Zaree T. 
Published: December 2014

A compact lowpass filter using a hexangular shaped resonator is presented. The designed lowpass filter has a −3 dB cutoff frequency of 2.97 GHz. The stopband bandwidth for the attenuation level of −20 dB is from 3.32 GHz up to 21 GHz, and the circuit only occupies 10.04 × 10.92 mm2. The structure is simulated, fabricated and measured. Both simulation and measured results are presented and compared and there is good agreement between them. The insertion loss in the passband is <0.06 dB and the fabricated filter presents a sharp transition band from 2.97 to 3.32 GHz for the attenuation level of −3 and −20 dB, respectively.
Fabrication of thermal‐based vacuum gauge
Authors: Jung Ho , Kim Tae Hyun , Kim Kyung Tae , Park Jae Hong , Kim Hee Yeoun 
Published: December 2014

The fabrication and evaluation of a wide‐range vacuum gauge for monitoring the pressure level inside an infrared focal plane array and a wafer‐level packaging (WLP) are reported. The proposed vacuum gauge has a microbolometer structure produced using the conventional surface micromachining process to achieve thermal isolation and high sensitivity. This structure has other advantages such as fast response time, a wider measurement range and easier integration to a Si substrate compared with other pressure sensors. The evaluation results show that the fabricated vacuum gauge has a linear dynamic range and a sensitivity of 10−3 to 105K/W/torr for vacuum pressures ranging from 10−6 to 760 torr. Also, the response time to vacuum change is reduced from 0.11 s at 10−5 torr to 15 ms at 100 torr. Therefore, the microbolometer‐based vacuum gauge has good potential for application in WLP, and it is possible to hermetically seal it with various read‐out integrated circuit substrates.
Autophagy regulates the stability of sialin, a lysosomal sialic acid transporter
Authors: Huang Chengcheng , Seino Junichi , Wang Li , Haga Yoshimi , Suzuki Tadashi 
Published: December 2014

Abstract
Macroautophagy plays a critical role in catabolizing cytosolic components via lysosomal degradation. Recent findings from our studies indicate that basal autophagy is required for the efficient lysosomal catabolism of sialyloligosaccharides, and that the downregulation of sialin, a lysosomal transporter of sialic acids can cause a significant delay in the cytosolic accumulation of such glycans. The findings reported herein show that the sialin protein level was increased when the autophagy process was inhibited. This effect appears to be specific to sialin, since the amount of LAMP1, another lysosomal membrane protein, remains constant under the same conditions. Our results suggest that autophagy may regulate the stability of sialin, and it could lead to the cytosolic accumulation of sialyloligosaccharides in autophagy-defective cells.
Lignans from the stems and leaves of Brandisia hancei and their effects on VEGF-induced vascular permeability and migration of HRECs and DLAV formation in zebrafish
Authors: Lee Ik-Soo , Kim Young Sook , Jung Seung-Hyun , Yu Song Yi , Kim Joo-Hwan , Sun Hang , Kim Jin Sook 
Published: December 2014

Abstract
In our continuing search for novel antiangiogenic agents, a new lignan glycoside, (7R,8R)-1-(4-O-β-d-glucopyranosyl-3-methoxyphenyl)-2-{2-methoxy-4-[1-(E)-propene-3-ol]-phenoxyl}-propane-1,3-diol (1), along with three known lignans (2–4), were isolated from the 80% EtOH extract of Brandisia hancei stems and leaves. These isolates (1–4) were subjected to an in vitro bioassay to evaluate their effects on vascular endothelial growth factor (VEGF)-induced vascular permeability and migration of human retinal endothelial cells (HRECs). Of the compounds tested, compound 1 resulted in the greatest reduction in VEGF-induced vascular permeability by about 31.5% at 10 μM compared to the VEGF-treated control. In the migration assay, compounds 1 and 2 significantly decreased VEGF-induced HREC migration. Furthermore, zebrafish embryos treated with compounds 1 and 2 showed mild reductions of dorsal longitudinal anastomotic vessel (DLAV) formation.
Pyroglutamic acid stimulates DNA synthesis in rat primary hepatocytes through the mitogen-activated protein kinase pathway
Authors: Inoue Shinjiro , Okita Yoichi , de Toledo Andreia , Miyazaki Hiroyuki , Hirano Eiichi , Morinaga Tetsuo 
Published: December 2014

Abstract
We purified pyroglutamic acid from human placental extract and identified it as a potent stimulator of rat primary hepatocyte DNA synthesis. Pyroglutamic acid dose-dependently stimulated DNA synthesis, and this effect was inhibited by PD98059, a dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1) inhibitor. Therefore, pyroglutamic acid stimulated DNA synthesis in rat primary hepatocytes via MAPK signaling.
Initial Characterization of Embryonic Development in North American Burbot
Authors: Egan Joshua P , Johnson Ryan D , Anders Paul J , Cain Kenneth D 
Published: December 2014

Abstract
Declining wild populations and increasing commercial interest are encouraging development of aquaculture methods for the North American Burbot Lota lota maculosa. A current focus of Burbot aquaculture research is reducing high mortality that is typically associated with culture of Burbot embryos and larvae. However, no Burbot embryonic or larval staging systems are currently available to provide a comparative baseline for studies of these life stages. To help address this gap, we examined Burbot embryonic development from egg fertilization until the onset of the larval period at first feeding. Ontogeny was characterized using diagnostic morphological features visible with stereo microscopy. Six developmental periods were characterized (cleavage, blastula, gastrula, segmentation, swim bladder inflation, and first feeding), along with 15 developmental stages. Water temperature ranged from 3°C to 5°C for the duration of this study. Cleavage cycles were approximately 8 h/division. Segmentation began at 12 d postfertilization (DPF) and continued until first hatch at 33 DPF. First feeding was observed at 45 DPF, 12 d after first hatch. Results presented here are expected to help refine Burbot culture methodology and research efforts aimed at conservation, management, and commercial production. Future research should use additional cohorts and expose embryos to a range of culture conditions to better understand the factors governing embryonic survival and variability in the timing of events during development.
Effect of Air Exposure and Resubmersion on the Behavior and Oxidative Stress of Pacific White Shrimp Litopenaeus vannamei
Authors: Liu Hui-Ling , Yang Shi-Ping , Wang Cheng-Gui , Chan Siu-Ming , Wang Wang-Xiong , Feng Zhen-Hua , Sun Cheng-Bo 
Published: December 2014

Abstract
The effect of air exposure on antioxidant activities in Pacific white shrimp Litopenaeus vannamei was studied. The behavioral changes in the shrimp and the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and total antioxidant competence (T-AOC) in the muscles and hepatopancreas were determined after the shrimp were exposed to air and then resubmersed in water. Results showed that the duration of air exposure significantly influenced shrimp survival. The maximum air exposure period during which the shrimp could remain alive was 30 min. After 10 min of air exposure, the shrimp could survive when they were resubmersed in water. The T-AOC in the hepatopancreas and muscles was significantly decreased in shrimp that were exposed to air for 20 min. The MDA content in the hepatopancreas was significantly higher for the 20-, 30-, and 40-min air exposure groups than for the control group. During the resubmersion period, the MDA content in the shrimp hepatopancreas and muscles increased. For shrimp that were exposed to air for 10 min, SOD activities in the hepatopancreas and muscles were restored after 3 h of resubmersion in water. Our results indicate that air exposure can cause oxidative damage to Pacific white shrimp, but the damage can be reversed after the shrimp are resubmersed.
Application of a single‐colony coculture technique to the isolation of hitherto unculturable gut bacteria
Authors: Tanaka Yoshiki , Benno Yoshimi 
Published: December 2014

ABSTRACTMolecular studies have led to postulation of a relationship between gut microbiota and certain diseases. However, because studies of hitherto uncultured species in vivo are essential for characterizing the biology and pathogenic properties of gut bacteria, techniques for culturing and isolating such bacteria must be developed. Here, a technique is described that partially overcomes the obstacles that prevent detection of interbacterial communication in vitro and are thus responsible for the failure to culture certain bacterial species. For this purpose, a ring with a membrane filter at the bottom was designed and a relatively simple nutrient medium was used instead of conventional media. Gut bacteria were cocultivated in soft agar separated by the membrane filter to simulate interbacterial communication in vitro. Use of this soft agar coculture technique led to the successful isolation of hitherto uncultured bacteria and the demonstration of multistage interbacterial communication among gut bacteria in vitro. Cultivation and isolation of single colonies of bacteria that require other bacteria for growth will enhance efforts to better understand the physiological and pathogenic roles of gut microbiota.