bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of clinical image evaluation charts for panoramic radiography
Authors: Kim Yeonhee , Lee Samsun , Jo Gyudong , Kwon Ahyoung , Kang Juhee , Kim Joeun , Huh Kyunghoe , Yi Wonjin , Heo Minsuk , Choi Soonchul 
Published: July 2024

Abstract
Objective
To compare and analyze professional (P chart) and simple (S chart) clinical image evaluation charts for evaluating panoramic radiograph image quality.

Methods
Ten evaluators assessed 285 clinical panoramic radiograph images. The evaluators were divided into oral and maxillofacial radiologists (OMFR, n = 5) and general dentist (dentists not specializing in oral and maxillofacial radiology, G, n = 5) groups. For image evaluation, P and S charts provided by the Korean Academy of Oral and Maxillofacial Radiology were used. Scores of items for each evaluation chart were used to compare the reliability, correlation, evaluation scores, evaluation time, and preference, and statistical analyses were performed using IBM SPSS Statistics.

Results
The S chart showed similar levels of evaluation scores at shorter evaluation time, as compared to the P chart. In the results for each evaluation chart, all analyzed correlations were statistically significant. Total score, image density/contrast/sharpness, and overall image quality items showed a very high positive correlation in the P chart. While the overall range of correlation coefficients was relatively lower in the S chart than the P chart, the same items showed high correlation coefficients. In the preference evaluation, both the professional and generalist groups preferred the S chart.

Conclusions
A comparative analysis with the P chart, revisions, and upgrades are needed for the S chart items that showed low correlations in this study, such as artifacts, coverage area, and patient movement.
Developing evidence-based clinical imaging guidelines for the diagnosis of vertically fractured teeth
Authors: Kim Ki-Hong , Kim Jo-Eun , Lee Sam-Sun , Lee Chena , Choi Miyoung , Yong Hwan Seok , Jung Seung Eun , Heo Min-Suk , Huh Kyung-Hoe 
Published: July 2024

Abstract
Objectives
This study aimed to develop an evidence-based clinical imaging guideline for teeth suspected with vertical root fractures.

Methods
An adaptation methodology based on the Korean Clinical Imaging Guidelines (K-CIG) was used in the guideline development process. After searching for guidelines using major databases such as Ovid-Medline, Elsevier-Embase, National Guideline Clearinghouse, and Guideline International Network, as well as domestic databases such as KoreaMed, KMbase, and KoMGI, two reviewers analyzed the retrieved articles. The retrieved articles were included in this review using well-established inclusion criteria.

Results
Twenty articles were identified through an online search, of which three were selected for guideline development. Based on these three guidelines, this study developed specific recommendations concerning the optimal imaging modality for diagnosing teeth suspected of vertical root fractures.

Conclusions
Periapical radiography is the preferred method for assessing teeth with mastication-related pain and suspected vertical root fractures. However, if intraoral radiographs do not provide sufficient information about root fractures, a small FOV CBCT may be considered. However, the use of CBCT in endodontically treated teeth is significantly constrained by the presence of artificial shading.
The current knowledge about the distribution of Portulaca species (Portulacaceae) within the Balkan Peninsula
Authors: Peregrym Mykyta , Dragićević Snežana 
Published: July 2024

AbstractThis study addresses the complex taxonomy and distribution of the genus Portulaca L. (Portulacaceae) within the Balkans, focusing on the often-debated species composition and distribution patterns in the P. oleracea aggregate. Our research aims to resolve this ambiguity by addressing two key questions: 1) Which Portulaca taxa are present in the Balkans, and 2) What is the current distribution of these taxa here? To achieve this, we conducted literature reviews, seed collection, species identification using micromorphological characteristics, and distribution analysis. Our findings reveal the presence of four distinct species in the Balkans: P. oleracea agg., P. grandiflora, P. pilosa and P. umbraticola. Additionally, within the P. oleracea complex, twelve microspecies have been identified, primarily differentiated based on seed micromorphology. Besides, five species (P. daninii, P. granulato-stellulata, P. rausii, P. sardoa, and P. trituberculata) have been documented in Montenegro, and one species (P. nitida) – in Slovenia for the first time. This study provides a valuable contribution to the botanical knowledge of the Balkan Peninsula, offering the recommendations for future studies of the P. oleracea complex for the solution of the taxonomic puzzle in this aggregate.
Systematic Mendelian Randomization Exploring Druggable Genes for Hemorrhagic Strokes
Authors: Yang Lun-Zhe , Yang Yong , Hong Chuan , Wu Qi-Zhe , Shi Xiong-Jie , Liu Yi-Lin , Chen Guang-Zhong 
Published: July 2024

AbstractPatients with hemorrhagic stroke have high rates of morbidity and mortality, and drugs for prevention are very limited. Mendelian randomization (MR) analysis can increase the success rate of drug development by providing genetic evidence. Previous MR analyses only analyzed the role of individual drug target genes in hemorrhagic stroke; therefore, we used MR analysis to systematically explore the druggable genes for hemorrhagic stroke. We sequentially performed summary-data-based MR analysis and two-sample MR analysis to assess the associations of all genes within the database with intracranial aneurysm, intracerebral hemorrhage, and their subtypes. Validated genes were further analyzed by colocalization. Only genes that were positive in all three analyses and were druggable were considered desirable genes. We also explored the mediators of genes affecting hemorrhagic stroke incidence. Finally, the associations of druggable genes with other cardiovascular diseases were analyzed to assess potential side effects. We identified 56 genes that significantly affected hemorrhagic stroke incidence. Moreover, TNFSF12, SLC22A4, SPARC, KL, RELT, and ADORA3 were found to be druggable. The inhibition of TNFSF12, SLC22A4, and SPARC can reduce the risk of intracranial aneurysm, subarachnoid hemorrhage, and intracerebral hemorrhage. Gene-induced hypertension may be a potential mechanism by which these genes cause hemorrhagic stroke. We also found that blocking these genes may cause side effects, such as ischemic stroke and its subtypes. Our study revealed that six druggable genes were associated with hemorrhagic stroke, and the inhibition of TNFSF12, SLC22A4, and SPARC had preventive effects against hemorrhagic strokes.
Graphical Abstract
Detection of polyreactive immunoglobulin G facilitates diagnosis in children with autoimmune hepatitis
Authors: Engel Bastian , Diestelhorst Jana , Hupa-Breier Katharina Luise , Kirchner Theresa , Henjes Nicole , Loges Stephanie , Yuksel Muhammed , Janczyk Wojciech , Lalanne Claudine , Zachou Kalliopi , Oo Ye H. , Gournay Jérôme , Pape Simon , Drenth Joost P. H. , Renand Amédée , Dalekos George N. , Muratori Luigi , Socha Piotr , Ma Yun , Arikan Cigdem , Baumann Ulrich , Manns Michael P. , Wedemeyer Heiner , Junge Norman , Jaeckel Elmar , Taubert Richard 
Published: July 2024

Abstract
Objective
The detection of autoantibodies is essential to diagnose autoimmune hepatitis (AIH). Particularly in children, specificity of autoantibodies decreases due to lower titers being diagnostic and being present not only in AIH but also in other liver diseases. Recently, quantification of polyreactive IgG (pIgG) for detection of adult AIH showed the highest overall accuracy compared to antinuclear antibodies (ANA), anti-smooth muscle antibodies (anti-SMA), anti-liver kidney microsomal antibodies (anti-LKM) and anti-soluble liver antigen/liver pancreas antibodies (anti-SLA/LP). We aimed to evaluate the diagnostic value of pIgG for pediatric AIH.

Design
pIgG, quantified using HIP1R/BSA coated ELISA, and immunofluorescence on rodent tissue sections were performed centrally. The diagnostic fidelity to diagnose AIH was compared to conventional autoantibodies of AIH in training and validation cohorts from a retrospective, European multi-center cohort from nine centers from eight European countries composed of existing biorepositories from expert centers (n = 285).

Results
IgG from pediatric AIH patients exhibited increased polyreactivity to multiple protein and non-protein substrates compared to non-AIH liver diseases and healthy children. pIgG had an AUC of 0.900 to distinguish AIH from non-AIH liver diseases. pIgG had a 31–73% higher specificity than ANA and anti-SMA and comparable sensitivity that was 6–20 times higher than of anti-SLA/LP, anti-LC1 and anti-LKM. pIgG had a 21–34% higher accuracy than conventional autoantibodies, was positive in 43–75% of children with AIH and normal IgG and independent from treatment response.

Conclusion
Detecting pIgG improves the diagnostic evaluation of pediatric AIH compared to conventional autoantibodies, primarily owing to higher accuracy and specificity.

Graphical Abstract
Examining the experiences related to the psychological needs and future perceptions of Turkish adolescents on the basis of reality therapy: a qualitative study
Authors: Dursun Asiye , Canbulat Nergis 
Published: July 2024

AbstractThis qualitative study investigates adolescents’ perceptions and psychological needs regarding the future in the perspective of reality therapy. Employing a case study design as a qualitative research method, the study reached a total of 181 adolescent participants, including 125 (69.1%) girls and 56 (39.1%) boys. The data obtained from participants were analyzed using thematic analysis by the researchers. As a result of the analysis, their perceptions of the future were categorized into two themes: “Future Perception” and “Future-Oriented WDEP System.” Additionally, it is observed that they formulated various metaphors related to psychological needs. Adolescents’ perceptions of the future encompass both positive emotions, thoughts, and actions, as well as negative expressions. The findings of this research provide insights for developmental experts and mental health professionals in understanding the psychological needs and future perceptions of individuals during the crucial stage of adolescence.
Magnetoactive, Kirigami-Inspired Hammocks to Probe Lung Epithelial Cell Function
Authors: Wei Katherine , Roy Avinava , Ejike Sonia , Eiken Madeline K. , Plaster Eleanor M. , Shi Alan , Shtein Max , Loebel Claudia 
Published: July 2024

Abstract
Introduction
Mechanical forces provide critical biological signals to cells. Within the distal lung, tensile forces act across the basement membrane and epithelial cells atop. Stretching devices have supported studies of mechanical forces in distal lung epithelium to gain mechanistic insights into pulmonary diseases. However, the integration of curvature into devices applying mechanical forces onto lung epithelial cell monolayers has remained challenging. To address this, we developed a hammock-shaped platform that offers desired curvature and mechanical forces to lung epithelial monolayers.

Methods
We developed hammocks using polyethylene terephthalate (PET)-based membranes and magnetic-particle modified silicone elastomer films within a 48-well plate that mimic the alveolar curvature and tensile forces during breathing. These hammocks were engineered and characterized for mechanical and cell-adhesive properties to facilitate cell culture. Using human small airway epithelial cells (SAECs), we measured monolayer formation and mechanosensing using F-Actin staining and immunofluorescence for cytokeratin to visualize intermediate filaments.

Results
We demonstrate a multi-functional design that facilitates a range of curvatures along with the incorporation of magnetic elements for dynamic actuation to induce mechanical forces. Using this system, we then showed that SAECs remain viable, proliferate, and form an epithelial cell monolayer across the entire hammock. By further applying mechanical stimulation via magnetic actuation, we observed an increase in proliferation and strengthening of the cytoskeleton, suggesting an increase in mechanosensing.

Conclusion
This hammock strategy provides an easily accessible and tunable cell culture platform for mimicking distal lung mechanical forces in vitro. We anticipate the promise of this culture platform for mechanistic studies, multi-modal stimulation, and drug or small molecule testing, extendable to other cell types and organ systems.
A comprehensive review of membrane-based water filtration techniques
Authors: Aziz Shahid , Mazhar Abdur Rehman , Ubaid Ali , Shah Syed Muhammad Hamza , Riaz Yasser , Talha Tariq , Jung Dong-Won 
Published: July 2024

AbstractThe purification of water is not only essential for human consumption but is becoming a necessity considering the limited freshwater reserves of the planet. Over the last few decades advancements in material sciences and technology have paved the way for the development of novel purification techniques. Amongst these techniques membrane-based filtration is considered as the least expensive and most effective. These membrane-based filtration techniques can be broadly categorized into reverse osmosis (RO), ultrafiltration, microfiltration and activated carbon filters (ACF). The mode of operation, research evolution and practical applications of each technique are compared in this holistic analysis. Although RO is the oldest and most established membrane-based filtration technique in the literature, it is ACF that is ranked as the most promising new technique with much simplicity and effectiveness.
Machine learning-augmented multi-arrayed fiber bragg grating sensors for enhanced structural health monitoring by discriminating strain and temperature variations
Authors: Saha S. , Hadigheh S. A. , Rukhlenko I. , Valix M. , Uy B. , Fleming S. 
Published: July 2024

AbstractFiber optic sensors (FOS) in long-term structural health monitoring (SHM) have drawn significant attention due to their pivotal role in detecting defects and measuring structural performance in diverse infrastructures. While using FOS, temperature variation due to environmental factors is still considered one of the major challenges to isolating sensing parameters. To address this issue, we reported a machine learning (ML)-augmented multi-parameter sensing system that enables simultaneous detection of strain and temperature effects based on one single fiber Bragg gratings (FBGs) sensor for SHM. The initial phase entailed designing, fabricating, and characterizing a novel FBG sensor in the laboratory, incorporating a set of four FBGs, each distinguished by distinct Bragg wavelengths. In the next phase, ML algorithms are employed to separate temperature effects from strain variations. As a proof of concept, mechanical loading tests are conducted on the sensor, exposing the FBG portion to various temperature conditions. In the final phase, data collected from a post-tensioned concrete bridge embedded with both strain and temperature FBG sensors are utilized, and the developed ML models are applied to observe real-environment outcomes. Despite the limited feature points of collected FBG spectrums, the developed ML models effectively address cross-sensitivity issues induced by temperature perturbations. The long-term benefit of using FOS is that it will enable a better understanding and utilization of aging infrastructure. This will potentially reduce embodied carbon of infrastructure in the future and assist in the global efforts to achieve Net-Zero.
Analysis of starch content and multi-scale structure of reconstituted cut stems in tobacco
Authors: Mao Wenlong , Yao Zheng , Zhang Hua , Liao Huiyun , Li Jiang , Xue Dong , Yuan Guangxiang , Xu Baoming , Dou Kunpeng , Sheng Shijie , Xu Chao , Pang Junran , Tan Xiaoyan , Chi Bo , Xiong Dangan 
Published: July 2024

AbstractOn the basis of the relationship between the composition of the reconstituted cut stems and their functional positioning in the leaf formulation, this study improves the proportion of high-quality tobacco products by investigating the material basis of the effect of reconstituted cut stems on the quality of cigarette products, by characterizing the starch content, physicochemical properties, and characteristic structures of different components in tobacco products. The results showed that the starch content in reconstituted cut stems (4.93 ± 0.27%) was between high-quality tobacco leaves (4.48 ± 0.17%) and cut stems (5.13 ± 0.18%), indicating that the reduction of starch content during the processing of reconstituted cut stems is more conducive to the high-value treatment of reconstituted cut stems. At the same time, through the evaluation of the physico-chemical properties and multi-scale structural characteristics of starch particles, it was found that the starch of the reconstituted cut stems has a rock-like particle structure, and the short-range ordering on the surface increases, forming more ordered structural domains. In addition, the processed reconstituted cut stems increase the crystallinity of the starch. It also exhibits the typical B-type crystalline structure of starch, with stronger molecular chain interactions and high crystalline ordered arrangement. This study will provide technical guidance and theoretical support for improving the quality of reconstituted cut stems products, improving the bioavailability of tobacco products, reducing raw material costs, and effectively reducing the starch content of tobacco in the development of tobacco products.
Extracellular Vesicles from Adipose Tissue-Derived Stromal Cells Stimulate Angiogenesis in a Scaffold-Dependent Fashion
Authors: Getova V. E. , Orozco-García E. , Palmers S. , Krenning G. , Narvaez-Sanchez R. , Harmsen M. C. 
Published: July 2024

Abstract
Background:
The extracellular vesicles (EVs) secreted by adipose tissue-derived stromal cells (ASC) are microenvironment modulators in tissue regeneration by releasing their molecular cargo, including miRNAs. However, the influence of ASC-derived extracellular vesicles (ASC-EVs) on endothelial cells (ECs) and vascularisation is poorly understood. The present study aimed to determine the pro-angiogenic effects of ASC-EVs and explore their miRNA profile.

Methods:
EVs were isolated from normoxic and hypoxic cultured ASC conditioned culture medium. The miRNA expression profile was determined by miRseq, and EV markers were determined by Western blot and immunofluorescence staining. The uptake dynamics of fluorescently labelled EVs were monitored for 24 h. ASC-EVs' pro-angiogenic effect was assessed by sprouting ex vivo rat aorta rings in left ventricular-decellularized extracellular matrix (LV dECM) hydrogel or basement membrane hydrogel (Geltrex®).

Results:
ASC-EVs augmented vascular network formation by aorta rings. The vascular network topology and stability were influenced in a hydrogel scaffold-dependent fashion. The ASC-EVs were enriched for several miRNA families/clusters, including Let-7 and miR-23/27/24. The miRNA-1290 was the highest enriched non-clustered miRNA, accounting for almost 20% of all reads in hypoxia EVs.

Conclusion:
Our study revealed that ASC-EVs augment in vitro and ex vivo vascularisation, likely due to the enriched pro-angiogenic miRNAs in EVs, particularly miR-1290. Our results show promise for regenerative and revascularisation therapies based on ASC-EV-loaded ECM hydrogels.
Predicting brace holiday eligibility in juvenile idiopathic scoliosis
Authors: Lee Julianna , Chaclas Nathan , Hauth Lucas , VanEenenaam David , Desai Vineet , Flynn John M. 
Published: July 2024

Abstract
Purpose
The psychological effects of scoliosis bracing can be difficult, and thus clinicians sometimes recommend a brace holiday when the curve corrects to less than 25°. However, the clinical indications for taking a break from the brace before reaching maturity have yet to be described. We hypothesized there would be a relationship between brace holiday eligibility and degree of curve at presentation, change in curve magnitude while bracing, and level of bracing compliance.

Methods
A retrospective cohort study at a single institution was performed from 2016 to 2022. Objective brace compliance I-button data were collected on patients aged 3–9 years old. Patients with other etiologies besides idiopathic scoliosis before the age of 10 were excluded. Binary logistic regression was performed to determine the effect of significant variables on the likelihood of brace holiday.

Results
Fifty-six patients met inclusion criteria. Of these, 20 were able to get a brace holiday. Patients with higher brace compliance and larger in-brace curve correction were more likely to get a brace holiday (P = 0.015, 0.004). Patients with higher BMIs and larger curves at initial presentation were less likely to get a brace holiday (P = 0.002, 0.014).

Conclusion
Compliant brace wearers with good in-brace correction are most likely to be eligible for a brace holiday. While some elements remain immutable, others are modifiable, such as bracing compliance. Understanding how outcomes differ between patients who do and do not take a brace holiday will be crucial to elucidating if the psychological benefit of taking a break from the brace can be justified.
Screening of eating disorders and its related factors among Syrian undergraduates: a cross-sectional study
Authors: Alchallah Mhd Obai , Alolabi Homam , Safiah Mhd Homam , Ataya Sham , Alashabi Khaled Kalalib , Haffar Massa , Alistwani Dima , Alolabi Hani , Saeid Dana , Mohsen Fatema 
Published: July 2024

Abstract
Background
Eating disorders (EDs) are a complex group of disorders that affect mental and physical health and pose a challenge for medical professionals to diagnose. This study aims to screen for eating disorders among Syrian university undergraduates.

Methods and materials
A cross-sectional study was conducted among Syrian university students using a self-administered questionnaire. The questionnaire consisted of three sections: socio-demographic characteristics, dietary habits and barriers to healthy eating, and the Sick, Control, One, Fat, Food (SCOFF) score. The chi-square test was used to evaluate the factors related to eating disorders.

Results
Of 728 students, 45.3% were males and 54.7% females. The majority of participants studied medicine 32.3%, followed by pharmacy 25.7% and dentistry 17.6%. Overweight and obese participants represented 21.4% and 6.8% respectively. Merely 36% of students reported adopting a healthy diet, a greater percentage of females 58.8% reported having snacks compared to males 48.5% (P = 0.006), a higher percentage of females 38.8% reported consuming fast food compared to males 26.6% (P < 0.001). Most of the students' eating habits worsened during exams 55.7%, females 60.6% markedly reported worse eating habits during exams compared to males 50% (P = 0.017).
Lack of time 72.7%, not accessible 38.3%, and unenjoyable 31.6% were frequent barriers to healthy food. About 20.3% of students screened positive for probable eating disorders. Factors that elevate the likelihood of acquiring an ED encompass being female, having a lower or higher BMI, eating worse during exams, and psychological behaviors.

Conclusions
Probable EDs were prevalent among Syrian undergraduates. The addressed socio-demographic and psychological factors were significantly affecting students’ eating behaviors. These findings can be used to provide targeted lifestyle modification advice via health promotion programs for these specific groups.