bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of Mediator complex subunit MED15 in testicular germ cell tumors
Authors: Klümper Niklas , Syring Isabella , Offermann Anne , Adler David , Vogel Wenzel , Müller Stefan C. , Ellinger Jörg , Strauß Arne , Radzun Heinz Joachim , Ströbel Philipp , Brägelmann Johannes , Perner Sven , Bremmer Felix 
Published: September 2015

Abstract
Background
Testicular germ cell tumors (TGCT) are the most common cancer entities in young men with increasing incidence observed in the last decades. For therapeutic management it is important, that TGCT are divided into several histological subtypes. MED15 is part of the multiprotein Mediator complex which presents an integrative hub for transcriptional regulation and is known to be deregulated in several malignancies, such as prostate cancer and bladder cancer role, whereas the role of the Mediator complex in TGCT has not been investigated so far. Aim of the study was to investigate the implication of MED15 in TGCT development and its stratification into histological subtypes.

Methods
Immunohistochemical staining (IHC) against Mediator complex subunit MED15 was conducted on a TGCT cohort containing tumor-free testis (n = 35), intratubular germ cell neoplasia unclassified (IGCNU, n = 14), seminomas (SEM, n = 107) and non-seminomatous germ cell tumors (NSGCT, n = 42), further subdivided into embryonic carcinomas (EC, n = 30), yolk sac tumors (YST, n = 5), chorionic carcinomas (CC, n = 5) and teratomas (TER, n = 2). Quantification of MED15 protein expression was performed through IHC followed by semi-quantitative image analysis using the Definiens software.

Results
In tumor-free seminiferous tubules, MED15 protein expression was absent or only low expressed in spermatogonia. Interestingly, the precursor lesions IGCNU exhibited heterogeneous but partly very strong MED15 expression. SEM weakly express the Mediator complex subunit MED15, whereas NSGCT and especially EC show significantly enhanced expression compared to tumor-free testis.

Conclusions
In conclusion, MED15 is differentially expressed in tumor-free testis and TGCT. While MED15 is absent or low in tumor-free testis and SEM, NSGCT highly express MED15, hinting at the diagnostic potential of this marker to distinguish between SEM and NSGCT. Further, the precursor lesion IGCNU showed increased nuclear MED15 expression in the preinvasive precursor cells, which may provide diagnostic value to distinguish between benign and pre-malignant testicular specimen, and may indicate a role for MED15 in carcinogenesis in TGCT.
Long-term outcome of severe herpes simplex encephalitis: a population-based observational study
Authors: Jouan Youenn , Grammatico-Guillon Leslie , Espitalier Fabien , Cazals Xavier , François Patrick , Guillon Antoine 
Published: September 2015

Abstract
Introduction
Herpes simplex encephalitis (HSE) is a rare disease with a poor prognosis. No recent evaluation of hospital incidence, acute mortality and morbidity is available. In particular, decompressive craniectomy has rarely been proposed in cases of life-threatening HSE with temporal herniation, in the absence of evidence. This study aimed to assess the hospital incidence and mortality of HSE, and to evaluate the characteristics, management, the potential value of decompressive craniectomy and the outcome of patients with HSE admitted to intensive care units (ICUs).

Methods
Epidemiological study: we used the hospital medical and administrative discharge database to identify hospital stays, deaths and ICU admissions relating to HSE in 39 hospitals, from 2010 to 2013. Retrospective monocentric cohort: all patients with HSE admitted to the ICU of the university hospital during the study were included. The use of decompressive craniectomy and long-term outcome were analyzed. The initial brain images were analyzed blind to outcome.

Results
The hospital incidence of HSE was 1.2/100,000 inhabitants per year, 32 % of the patients were admitted to ICUs and 17 % were mechanically ventilated. Hospital mortality was 5.5 % overall, but was as high as 11.9 % in ICUs. In the monocentric cohort, 87 % of the patients were still alive after one year but half of them had moderate to severe disability. Three patients had a high intracranial pressure (ICP) with brain herniation and eventually underwent decompressive hemicraniectomy. The one-year outcome of these patients did not seem to be different from that of the other patients. It was not possible to predict brain herniation reliably from the initial brain images.

Conclusions
HSE appears to be more frequent than historically reported. The high incidence we observed probably reflects improvements in diagnostic performance (routine use of PCR). Mortality during the acute phase and long-term disability appear to be stable. High ICP and brain herniation are rare, but must be monitored carefully, as initial brain imaging is not useful for identifying high-risk patients. Decompressive craniectomy may be a useful salvage procedure in cases of intractable high ICP.
The senescent methylome and its relationship with cancer, ageing and germline genetic variation in humans
Authors: Lowe Robert , Overhoff Marita G. , Ramagopalan Sreeram V. , Garbe James C. , Koh James , Stampfer Martha R. , Beach David H. , Rakyan Vardhman K. , Bishop Cleo L. 
Published: September 2015

Abstract
Background
Cellular senescence is a stable arrest of proliferation and is considered a key component of processes associated with carcinogenesis and other ageing-related phenotypes. Here, we perform methylome analysis of actively dividing and deeply senescent normal human epithelial cells.

Results
We identify senescence-associated differentially methylated positions (senDMPs) from multiple experiments using cells from one donor. We find that human senDMP epigenetic signatures are positively and significantly correlated with both cancer and ageing-associated methylation dynamics. We also identify germline genetic variants, including those associated with the p16INK4A locus, which are associated with the presence of in vivo senDMP signatures. Importantly, we also demonstrate that a single senDMP signature can be effectively reversed in a newly-developed protocol of transient senescence reversal.

Conclusions
The senDMP signature has significant potential for understanding some of the key (epi)genetic etiological factors that may lead to cancer and age-related diseases in humans.
Developmental attenuation of N-methyl-D-aspartate receptor subunit expression by microRNAs
Authors: Corbel Caroline , Hernandez Israel , Wu Bian , Kosik Kenneth S. 
Published: September 2015

Abstract

Background
N-methyl-D-aspartate receptors (NMDARs) are a subtype of ionotropic glutamate receptors and are expressed throughout the central nervous system (CNS). Their activity is required for excitatory synaptic transmission, the developmental refinement of neural circuits and for the expression of many forms of synaptic plasticity. NMDARs are obligate heterotetramers and the expression of their constituent subunits is developmentally and anatomically regulated. In rodent cortex and hippocampus, the GluN2B subunit is expressed at high levels early in development and decreases to plateau levels later while expression of the GluN2A subunit has a concomitant increase. Regulation of GluN2A and GluN2B expressions are incompletely understood. Here, we showed the influence of miRNAs in this process.

Findings
Two miRNAs, miR-19a and miR-539 can influence the levels of NMDARs subunits, as they target the mRNAs encoding GluN2A and GluN2B respectively. MiR-539 also modified the expression of the transcription factor REST, a known regulator of NMDAR subunit expression.

Conclusions
miR-19a and miR-539, in collaboration with REST, serve to set the levels of GluN2A and GluN2B precisely during development. These miRNAs offer an entry point for interventions that affect plasticity and a novel approach to treat neurodegenerative diseases.
On approximating Mills ratio
Authors: Yang Zhen-Hang , Chu Yu-Ming 
Published: September 2015

AbstractIn the article, we present several sharp bounds for the Mills ratio $R(x)=e^{x^{2}/2}\int_{x}^{\infty}e^{-t^{2}/2}\,dt$
R
(
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=

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∫
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($x>0$
x
>
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) in terms of the functions $I_{a}(x)=a/[\sqrt{x^{2}+2a}+(a-1)x]$

I
a

(
x
)
=
a
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[

x
2

+
2
a

+
(
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and $J(x)=a/[\sqrt{x^{2}+2a^{2}/\pi}+2ax/\pi]$
J
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x
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=
a
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[

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with parameter $a>0$
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.
Fixed point theorems with generalized altering distance functions in partially ordered metric spaces via w-distances and applications
Authors: Sang Yanbin , Meng Qian 
Published: September 2015

AbstractIn this paper, we establish some fixed point theorems with T-contractions and w-distances in partially ordered metric spaces. The main tool used in our proof is a generalized altering distance function. Our results can be applied directly to study multidimensional fixed point which covers the concepts of coupled, tripled, quadruple fixed point etc. Moreover, a Fredholm integral equation and an initial-value problem for partial differential equation of parabolic type are also discussed.
Common fixed point results for four maps satisfying ϕ-contractive condition in multiplicative metric spaces
Authors: Gu Feng , Cho Yeol Je 
Published: September 2015

AbstractIn this paper, we consider the setting of multiplicative metric spaces to establish results regarding the common fixed points of four mappings, using a contraction condition defined by means of a comparison function. Also, we provide illustrative examples in support of our new results. The results obtained in this paper extend and improve some well-known comparable results in the literature due to He et al. (Fixed Point Theory and Appl. 2013:48, 2013).
French and Italian co-productions and the limits of transnational cinema
Authors: Romanelli Claudia 
Published: September 2015

Abstract
This article revisits the concept of transnational cinema in light of the laws concerning French and Italian co-productions since the end of World War II, in an attempt to demonstrate how transnational modes of production negotiate with national modes at the level of financial resources and cultural exchange. It is generally assumed that, unlike national cinema, transnational cinema does not erect any boundaries between films produced in different nation states. However, the basic concern that co-production agreements promote is to assimilate co-produced films into a national culture under the direct tutelage of the nation state. Co-production treaties are made in the interest of domestic film markets and are revealing of a notion of cinema that reaches across borders while positing nationality as an imagined community. A definition of transnational cinema that is above and beyond the limits of the national is ultimately at odds with the limitations embedded in co-productions.
Studies on microstructure and mechanical properties of keyhole mode Nd:YAG laser welded Inconel 625 and duplex stainless steel, SAF 2205
Authors: Devendranath Ramkumar K. , Goutham Kumar P. Shiva , Sai Radhakrishna V. , Kothari Karan , Sridhar R. , Arivazhagan N. , Kuppan P. 
Published: September 2015

Abstract
Scaling and Root Planing per Quadrant Versus One‐Stage Full‐Mouth Disinfection: Assessment of the Impact of Chronic Periodontitis Treatment on Quality of Life — A Clinical Randomized, Controlled Trial
Authors: Santuchi Camila Carvalho , Cortelli José Roberto , Cortelli Sheila Cavalca , Cota Luís Otávio Miranda , Fonseca Douglas Campidelli , Alencar Camila Oliveira , Costa Fernando Oliveira 
Published: September 2015

Background: There are few randomized, controlled clinical trials about the effect of non‐surgical periodontal treatment on oral health–related quality of life (OHRQL). This study aims to compare the effects of two different forms of non‐surgical periodontal therapy, scaling and root planing (SRP) per quadrant and one‐stage full‐mouth disinfection (FMD), on periodontal clinical parameters and OHRQL of patients with chronic periodontitis.Methods: In this randomized, controlled clinical trial, the questionnaires Oral Impacts on Daily Performance (OIDP) and Oral Health and Quality of Life (OHQoL) were given to 90 patients divided into two groups: SRP (n = 45) and FMD (n = 45). Periodontal clinical parameters recorded included probing depth, clinical attachment level, plaque index, and gingival index. For statistical analysis, χ2 test, Fisher exact test, Mann‐Whitney U test, and Wilcoxon test were used. Intention‐to‐treat analyses were performed at T0 (baseline) for periodontal clinical parameters, T1 (30 days after treatment) for questionnaires, and T2 (180 days after treatment) for both.Results: No significant differences were identified between the SRP and FMD groups in regard to OHQoL and OIDP scores when comparing the data of T1 and T2.Conclusion: Patients treated by both SRP and FMD showed improvement in all periodontal clinical parameters and OHRQL, with no significant differences between treatment groups.
Comprehensive Investigation of Pressure Transient Analysis Methods of Wells Producing under Multiphase Flow Conditions
Authors: Mahran Shahenda , El-Banbi Ahmed H. 
Published: September 2015

Abstract
The development of pressure transient analysis was originally based on single phase flow for slightly compressible fluids. It was later extended to include dry gas flow and multiphase conditions.
In this work, we explore the accuracy of the three most commonly used multiphase pressure transient analysis methods [single phase analysis using composite model, Perrine Martin (P-M), and multiphase pseudo-pressure method] under various conditions. Our approach included using appropriate numerical simulation techniques to generate drawdown and buildup data for gas-oil system at variety of conditions of GOR, CGR. Then, each test was evaluated with the 3 well test analysis methods, and error between calculated and actual results was computed for each method. In each case, we compared the well test derived properties (e.g. permeability, skin, completion information) with actual properties (given from numerical reservoir simulation). We tested various oil and gas properties spanning different fluid types of gas condensates and volatile oils. The testing also included different common well testing models such as radial, limited entry, hydraulically fractured, and horizontal wells. We then embarked on detailed analysis of these errors to derive conclusions regarding which methods give more accurate results under which conditions.
As an example, application of the three analysis approaches to horizontal well models in gas condensate reservoirs using different actual fluid samples with varying composition, at different levels of drawdown; it was found that (1) single-phase with composite reservoir method underestimated the permeability by 8% for very lean gas-condensate and by 18% for rich gas condensate fluid sample, (2) Perrine Martin method was able to predict the permeability with error &lt;17% at low gas production rate, and (3) multiphase pseudo-pressure functions (steady state and three-zone) produced best results with permeability error less than 4% for lean gas-condensate samples, and by 16% for very rich gas condensates. Many other useful conclusions for different systems were also derived. These conclusions show the expected level of error for properties predicted from well test analysis under different conditions of multiphase and for different well test models. Using these conclusions, a list of guidelines was recommended for well test analysis in multiphase tests.
Ribavirin Concentration Determines Treatment success of First-Generation DAA-Based Chronic HCV Therapy
Authors: De Kanter Clara TMM , Buti Maria , DeMasi Ralph , Ouwerkerk-Mahadevan Sivi , Dofferhoff Anton SM , Witek James , Drenth Joost PH , Zeuzem Stefan , Burger David M 
Published: September 2015

Background Monitoring ribavirin concentrations during hepatitis C treatment with dual therapy can help optimize treatment response and minimize anaemia. A defined therapeutic range for ribavirin during direct-acting antiviral-based therapies is lacking. This analysis explores whether a therapeutic range for ribavirin concentrations can be defined in patients treated with boceprevir- or telaprevir-based triple therapies. Methods Treatment-naive patients from ADVANCE, ILLUMINATE, OPTIMIZE and SPRINT-2, and treatment-experienced patients from RESPOND-2 were included. Multivariable logistic regression analyses were performed to evaluate whether ribavirin concentrations were an independent predictor of sustained virological response or anaemia. Optimal cutoff values and the percentage of patients within the proposed therapeutic range were determined, along with the associated chance of response. Results Overall, 1,502 patients were included. In both regimens, ribavirin concentrations were significantly associated with anaemia (haemoglobin level <10 g/dl) at all time points (1.75 < odds ratio [OR] <2.45) and sustained virological response was associated with ribavirin concentrations at week 8 (OR=1.43 for telaprevir and 1.78 for boceprevir). A therapeutic range for ribavirin at week 8 of 2.2–3.5 mg/l was defined for telaprevir treatment. Of the 48% of patients with a concentration within this range, 81% achieved sustained virological response and only 5.1% reported anaemia. For boceprevir treatment, the week 8 optimal range was defined as 2.2–3.6 mg/l and 50% of patients had a concentration within this range, of whom 69% achieved sustained virological response and 46% developed anaemia. Conclusions We established the therapeutic range for ribavirin in boceprevir- and telaprevir-based therapy that balances safety and efficacy.
Recommendation for Gopher Tortoise Burrow Buffer to Avoid Collapse from Heavy Equipment
Authors: Smith Lora L. , Hinderliter Matt , Taylor R. Scott , Howze Jennifer M. 
Published: September 2015

AbstractGopher tortoises (Gopherus polyphemus) occur in open-canopy pine habitat on well-drained soils in the southeastern United States, where they construct burrows that offer protection from thermal extremes, fire, and predators. Gopher tortoise populations have declined over the past 50 y, primarily as a result of habitat loss and degradation. Southeastern pine forests require active management with prescribed fire, mechanical thinning, or removal of hardwoods to maintain suitable habitat for gopher tortoises. In addition, many pine forests in the Southeast that support gopher tortoise populations are managed for multiple uses including intensive silviculture. Heavy equipment associated with these activities used in proximity to gopher tortoise burrows can cause them to collapse, potentially causing harm to tortoises or other imperiled organisms that use their burrows. Hence, there is a need for practical guidelines for use of heavy equipment for timber harvest, management, and other activities around gopher tortoise burrows to minimize risk to tortoises. We conducted a field study to determine the distance at which heavy equipment caused gopher tortoise burrows to collapse using a feller buncher, rubber-tire front-end loader, and an agricultural tractor with a tree-mower attachment in sandy clay loam (15 burrows) and undifferentiated deep sand (15 burrows) soils at a site in southwestern Georgia. All burrows were confirmed to be unoccupied by tortoises or other vertebrate commensal species using a camera scope before collapse. The greatest mean distance to collapse across all vehicles tested in sandy clay loam and undifferentiated deep sand was 2.19 ± 0.56 m and the maximum distance to collapse was 3 m. Given the variation in collapse distance, we recommend a buffer that extends 4 m in radius from the entrance of the gopher tortoise burrow to minimize risk of collapse from heavy equipment.
Seismic expressions of shallow gas in the lacustrine deposits of Lake Balaton, Hungary
Authors: Visnovitz F. , Bodnár T. , Tóth Zs. , Spiess V. , Kudó I. , Timár G. , Horváth F. 
Published: September 2015

ABSTRACTLake Balaton, a large shallow lake in Central Europe (Hungary), has been the site of extensive ultra‐high‐resolution acoustic and multichannel seismic profiling in the period of 1997–2013. These surveys showed the widespread occurrence of shallow gas in the lake sediments and their immediate substrata. We analyzed about 2000 km of two‐dimensional profiles and mapped the different gas occurrences in the uppermost 20 m. The anomalies caused by free gas were identified, classified, and assigned to upper, middle and lower levels based on gas signatures and stratigraphic position. Monitoring of the uppermost gas front has revealed temporal variations between surveys from different years and seasons that manifested in the changes of free gas content in the upper two levels. Free gas in the lower part of the lake sediments and at around the base of the mud indicated greater stability. The different nature of the three free gas levels can be explained by vertical changes in quantity, production rate, and solubility of methane and carbon dioxide gases. We suggest that methane was derived from the microbial decomposition of organic matter in the mud and Pleistocene peat at the base of the mud, whereas is transported to the lower mud layers by upwelling fluids.
Near‐surface VS structure by inversion of surface wave estimated from ambient seismic noise
Authors: Shirzad Taghi , Shomali Z. Hossein , Naghavi Mojtaba , Norouzi Rahim 
Published: September 2015

ABSTRACTIn this study, 20 hours of ambient seismic noise recorded from a small‐scale inter‐station distance was used to obtain near‐surface shear wave velocity structures at a local test site in Tehran (Iran). High‐resolution group velocity dispersion curves using fundamental mode of surface waves were calculated for all possible combinations of station pairs at frequencies ranging from 1 Hz to 25 Hz. Unlike most previous studies regarding ambient seismic noise, which observe very little coherent noise at frequencies larger than 1 Hz, the empirical Green’s functions were extracted using a root‐mean‐square stacking method showing more coherent signals. Our results indicate that ambient seismic noise is a viable technique at a frequency range of 1 Hz–25 Hz even when different sensor types are present. One‐dimensional VSV and VSH models from the near surface were then assessed by inverting the calculated Rayleigh and Love waves’ dispersion measurements. We observed that the calculated shear wave velocity model agrees with the available downhole model and shows three distinct layers in the upper 25 m of the test site.
Magnetic gradient and ground penetrating radar prospecting of buried earthen archaeological remains at the Qocho City site in Turpan, China
Authors: Shi Zhanjie , Tian Gang , Hobbs Richard W. , Wo Haowei , Lin Jinxin , Wu Leyuan , Liu Haiyan 
Published: September 2015

ABSTRACTIn order to test the ability of geophysical technologies to detect buried structures made of mud brick and rammed earth, a geophysical survey was acquired at Qocho City site of China in 2012 using magnetic gradient and ground penetrating radar (GPR). Magnetic anomalies were interpreted as the response of house wall foundations, pits, and a temple base by reference to archaeological results from a neighbouring excavation area. The magnetic data were complemented by 2D ground penetrating radar profiles, which provided additional information on the depth of these causative structures. An archaeological survey dated 1913 reported the layout of three houses that have since been largely razed to the ground in the study area. Our geophysical survey confirmed the locations of two houses. This study shows that magnetic and ground penetrating radar methods are valuable tools to detect buried earthen archaeological remains in a dry environment.