bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular detection of two virulence factors of Pseudomonas aeruginosa isolated from burn patients
Authors: Dakhil Degaim Zainab , Khamis Al-Malk Haydar , Methqal Nassar Alaa , A. Nasir Ahmed 
Published: July 2024

The present study aimed to identify two virulence genes in P. aeruginosa isolated from infected burn wound. Out of 70 burn swabs, 54 (77%) showed positive growth on enriched media which obtained from burn wound at Al-Mussan Teaching Hospital in Mussan province, Iraq. Only 13 isolates (35%) identified as P. aeruginosa, and those isolates examined using Polymerase Chain Reaction (PCR) for detection the exoS and phzS genes. The results recorded that 46% and 77% of isolates have exoS, phzS genes, respectively. We concluded a P. aeruginosa harbor diverse percentages of both genes as virulence factors, and those factors must be increased the pathogenicity of this pathogen.
Study of the effect of fiber orientation on the flexural strength of epoxy-coated glass fiber-reinforced composite materials
Authors: M. Ali Hisham , R. Mohammed Abdurahman 
Published: July 2024

In this paper, a composite material was prepared with layers of glass fiber. Six layers were arranged with unidirectional angles In addition angles with tilted layers, any symmetrical angle-ply laminate three layers are .
In Hand Lay-up¸the composite material consists of epoxy resin as a base material and layers of fiberglass as a reinforced material with weight fracture of 25% and 12%.respectivty The research was carried out in two stages: First, the synthesis of the composite material of the samples were then processed at different temperatures .
In the second stage, the three-point bending test was performed on the samples. The results have shown that the composite material is varying with directions. In the orientation of the fibers, the strength reaches at maximum values i.e. the angles While in the direction of the angles The strength at the minimum values and the values of strength between these two extremes, i.e. angles ( ) As for the symmetrical ply Laminated, the strength increases with the presence of the(0°) layer.
Single Nucleotide Polymorphism of IL-2 Gene at Position +166 in Type 1 Diabetes of Iraqi Patients
Authors: Ibtesam, B. Hassan  
Published: July 2024

The present study aimed at investigating of frequency of polymorphism of interleukin-2 gene (IL2) at position+166 SNP) in Type 1 Diabetes and in healthy controls subjects 39 of Iraqi patients, (12 males & 27 females;1 5.65 ± 1.79 years) and 21 controls.(7 mail & 14 female ; 14.66 ± 3.43 years) attended the hospital in Baghdad for diagnosis and treatment during the period February 2015 – January 2016 were enrolled in this study, the polymorphism of IL2+166 was data waved by polymerase chain reaction-specific sequence primer (PCR-SSP) assay. The results showed a comparison IL2+166 genotypes & alleles between T1D patients & controls frequencies of TT genotype & T allele (71.69vs. 68.95%;P =0.589 respectively) There was a significant increase in patients contrast to controls, (51.38vs. 48.08%; P =0.312) and associated RR rates were 19.2% & 27.0%, respectively. And the related EF values were 1.56 & 1.60 .Similar observations were made in GG genotype. In contrast TG genotype & G allele (23.93 vs. 28.55%,P =1.000 respectively) Low frequency was observed in patients compared to healthy subjects (43.28 vs. 43.22%; P =0.312), and the related PF values were 0.56 & 0.62 , respectively. The results of the study indicate that IL2+166 SNP may have a role in the mechanism of etiopathogenic of T1D in the samples of Iraqi patients
Accuracy of diffusion-weighted magnetic resonance imaging in diagnosing malignant musculoskeletal tumours
Authors: Shirin Mahbuba , Rahman Selina , Chowdhury Parisa , Showkat Mst. Syeeda , Karki Bandita Paul , Al Azad Md. Salahuddin 
Published: July 2024

Background: Conventional magnetic resonance imaging (MRI) lacks specificity for differentiating several tumours. Combining advanced techniques like diffusion-weighted imaging (DW-MRI) with conventional MRI may enhance diagnostic accuracy. However, we do not have such data for Bangladeshi patients. This study aimed to examine the diagnostic accuracy of apparent diffusion coefficient values obtained by DW-MRI.
Methods: A cross-sectional study was conducted from July 2022 to June 2023 in the Department of Radiology and Imaging, Bangabandhu Sheikh Mujib Medical University (BSMMU). After collecting their baseline data, thirty-five patients with musculoskeletal tumours underwent DW-MRI and histopathology tests or fine needle aspiration cytology (FNAC). The apparent diffusion coefficient (ADC) values obtained by DW-MRI were examined for diagnostic accuracy against a standard of histopathology/FNAC.
Results: According to the gold standard (histopathology/FNAC), there were 28 patients with malignancy, and 7 had benign tumours. Their mean age was 33 (standard deviation, 17) years (range, 4 to 74 years). The mean ADC value was 0.86 ± 0.30×10-3 mm2/s. The malignant musculoskeletal tumour group had significantly lower ADC (0.79 ± 0.24 ×10-3 mm2/s) compared to the benign tumour group (1.15 ± 0.37×10-3 mm2/s.) (P = 0.04). The DW-MRI ADC categories correctly diagnosed 27 malignant and five benign tumours using a cut-off value of ≤ 1.1×10−3 mm2/s. DW-MRI had a sensitivity of 96.4% and a specificity of 71.4%. Diagnostic accuracy was 91.4% for detecting malignant musculoskeletal tumours.
Conclusions: Malignant musculoskeletal tumours have lower DW-MRI-derived ADC levels, demonstrating good diagnostic accuracy. However, a larger and more representative sample is needed before it is recommended for clinical practice.
ANALISIS FAKTOR YANG BERHUBUNGAN DENGAN PEMERIKSAAN TRIPLE ELIMINASI PADA IBU HAMIL
Authors: Indriani Ratna Fitri , Adyas Atikah , Arisandi William , Noviansyah Noviansyah , Karyus Aila 
Published: July 2024

Triple eliminasi merupakan suatu program eliminasi atau pengurangan penularan HIV, Sifilis, dan Hepatitis B dari ibu ke bayi yang dilakukan secara bersamaan.. Pada tahun 2021 dengan jumlah K1 di wilayah kerja Puskesmas sebanyak 306 ibu hamil, sedangkan yang melakukan pemeriksaan tripel eliminasi ke Puskesmas sebanyak hanya 15 ibu hamil (4,9%), dan diperoleh hasil HbsAg positif sebanyak 4 orang. Tujuan Penelitian adalah diketahui faktor yang berhubungan dengan pemeriksaan triple eliminasi pada ibu hamil di Wilayah Kerja Dinas Kesehatan Kabupaten Lampung Tengah.

Jenis penelitian kuantitatif dengan desain Crossectional. Populasi penelitian ini adalah seluruh ibu hamil yang berada di Wilayah Kerja Dinas Kesehatan Kabupaten Lampung Tengah berjumlah 7994 ibu hamil dengan sampel digunakan sebanyak 192 responden menggunakan teknik proportional sampling. Penelitian telah dilaksanakan di wilayah kerja Dinas Kesehatan Kabupaten Lampung Tengah pada bulan Februari - Maret 2023. Pengumpulan data menggunakan lembar kuesioner. Analisi data secara univariat, bivariat, dan multivariat.

Hasil penelitian diketahui ada hubungan pengetahuan (p-value = 0,009), akses (p-value = 0,001), peran petugas kesehatan (p-value = 0,000), dukungan suami (p-value = 0,001), dengan pemeriksaan triple eliminasi pada ibu hamil di Wilayah Kerja Dinas Kesehatan Kabupaten Lampung Tengah sedangkan tidak ada hubungan pendidikan (p-value = 0,712) dan pekerjaan (p-value = 3,153) dengan pemeriksaan triple eliminasi pada ibu hamil di Wilayah Kerja Dinas Kesehatan Kabupaten Lampung Tengah. Variabel peran petugas merupakan faktor dominan dengan p-value = 0,001, OR = 3,3417. Saran diharapkan petugas kesehatan dapat meningkatkan upaya promosi tentang pentingnya pemeriksaan Tripel Eliminasi setiap ibu hamil, seperti dengan melakukan pnyuluhan secara kontinyu atau berkelanjutan di kelas ibu atau di posyandu.
ANALISIS FAKTOR YANG MEMPENGARUHI PEMANFAATAN PELAYANAN KESEHATAN PADA PESERTA BPJS PENERIMA BANTUAN IURAN (PBI)
Authors: Fajriani Arini 
Published: July 2024

Masyarakat perlu memahami bahwa tujuan jaminan kesehatan yaitu untuk mempermudah masyarakat dalam mengakses pelayanan kesehatan yang bermutu dan sesuai dengan prosedur, banyak nya keluhan masyarakat mengenai pelayanan kesehatan terutama masyarakat pengguna BPJS PBI, keluhan-keluhan tersebut salah satunya kurang nya informasi tentang pelayanan yang ada. Tujuan Penelitian ini untuk menganalisis faktor yang mempengaruhi pemanfaatan pelayanan kesehatan pada peserta BPJS Penerima Bantuan Iuran di Kecamatan Siantar Martoba, Kota Pematangsiantar. Penelitian ini menggunakan pendekatan kuantitatif observasional dengan metode deskriptif pada periode bulan Juni 2023. Pengambilan data menggunakan pembagian kuesioner. Informan yang terlibat sejumlah 50 orang peserta BPJS Penerima Bantuan Iuran di Kecamatan Siantar Martoba Kota Pematangsiantar. Sampel dalam penelitian ini sebanyak 50 orang yang dilakukan dengan teknik pengambilan sampel secara cluster random sampling. Instrumen yang digunakan berupa kuisioner penelitian. Hasil penelitian menunjukkan tingkat pendidikan (p-value=0,038), pengetahuan (p-value=0,045), pekerjaan (p-value=0,100), sikap (p-value=0,036), persepsi kondisi kesehatan (p-value=0,390), informasi (p-value=0,000), dan keterjangkauan (p-value=0,090). Kesimpulan yang diperoleh dalam penelitian ini yaitu tidak terdapat hubungan yang signifikan antara pekerjaan, persepsi kondisi kesehatan dan keterjangkauan dengan pemanfaatan pelayanan kesehatan. Terdapat hubungan yang signifikan antara pendidikan, pengetahuan, sikap dan informasi dengan pemanfaatan pelayanan kesehatan. Hasil penelitian ini diharapkan dapat berguna dan menjadi evaluasi para tenaga kesehatan dalam memberikan pemanfaatan pelayanan kesehatan kepada peserta BPJS PBI mengingat kurangnya pemberian informasi dan komunikasi para tenaga kesehatan dengan masyarakat dalam meningkatkan akses dan keterjangkauan pelayanan kesehatan yang lebih baik.
Pelatihan Kader Kesehatan untuk Meningkatkan Keterampilan Deteksi Dini Stunting
Authors: Indanah Indanah , Jauhar Muhamad , Kartikasari Fitriana , Kusumawardani Lita Heni 
Published: July 2024

ENGLISHThe success of finding new cases of stunting is influenced by the ability of health cadres to carry out early detection. The purpose of the study was to identify the effect of health cadre training on stunting early detection skills. The research design used quasi-experiments with variables, namely health cadre training and stunting early detection skills. The population is all health cadres, and there is a sample of 33 health cadres for each intervention and control group. Sample selection is done using the purposive sampling technique. The study was conducted at two Puskesmas in Kudus Regency in November 2021. The research instrument uses observation sheets. Health cadres attended four sessions over two days with duration of 45 to 60 minutes per session in the form of lectures and demonstrations. Data analysis used Wilcoxon and Mann-Whitney tests. Health cadre training improves stunting early detection skills with p value = 0.000 (p<0.05). Increasing the capacity of health cadres to find new cases of stunting in the community can be done through routine coaching that is integrated with child health service programs in health service facilities. INDONESIAKeberhasilan penemuan kasus baru stunting di masyarakat dipengaruhi oleh kemampuan kader kesehatan dalam melakukan deteksi dini. Tujuan penelitian ini untuk mengidentifikasi pengaruh pelatihan kader kesehatan terhadap keterampilan deteksi dini stunting. Desain penelitian menggunakan quasi eksperimen dengan 2 variabel, yaitu pelatihan kader kesehatan dan keterampilan deteksi dini stunting. Populasi penelitian seluruh kader kesehatan dan sampel 33 kader kesehatan untuk masing-masing kelompok intervensi dan kontrol. Pemilihan sampel menggunakanteknik purposive sampling. Kriteria inklusi berupa kader kesehatan aktif, belum pernah mendapatkan pelatihan serupa, berusia dewasa, pendidikan minimal SD, mampu membaca dan menulis. Penelitian dilakukan di dua Puskesmas di Kabupaten Kudus pada bulan November 2021. Instrumen penelitian menggunakan lembar observasi. Kader kesehatan mengikuti empat sesi selama dua hari dengan durasi 45-60 menit/sesi dalam bentuk ceramah dan demonstrasi. Materi terdiri dari konsep tumbuh kembang, deteksi dini stunting, dan komunikasi informasi edukasi stunting. Analisis data menggunakan uji Wilcoxon dan Mann Whitney. Pelatihan kader kesehatan meningkatkan keterampilan deteksi dini stunting dengan nilai p = 0,000 (p<0,05). Peningkatan kapasitas kader kesehatan dalam menemukan kasus baru stunting di masyarakat dapat dilakukan melalui pembinaan rutin terintegrasi dengan program pelayanan kesehatan anak di fasilitas pelayanan kesehatan.
Analisis Efektivitas dan Potensi Peningkatan Penerimaan Pajak Bumi dan Bangunan terhadap Pendapatan Asli Daerah
Authors: Utomo Rohadi , Rahmaliza Rahmaliza 
Published: July 2024

ENGLISHDiversion the transfer of Land and Building Tax management from central to regional taxes is intended to increase Original Regional Income. This research aims to determine the effectiveness of increasing Land and Building Tax revenue for Original Regional Income in Lubuklinggau City, and to analyze of Land and Building Tax management in Lubuklinggau City, especially the potential for revenue originating from Land and Building Tax. To measure the level effectiveness of Land and Building Tax revenue, data analysis of the potential and realization PBB revenue is used, while measuring the potential for increasing Land and Building Tax revenue is done by analyzing the area of potential tax areas, the selling value of tax objects, and calculating the potential based on Land and Building Tax rates with the selling value of taxable tax objects. The results of research show that in 2017-2021, Land and Building Tax revenue's effectiveness level was categorized as ineffective because the percentage of Land and Building Tax revenue realization was <60%. In 2021, the effectiveness value was 39.63%. Meanwhile, the potential for increasing Land and Building Tax revenue is significant. The current realization for 2021 was only 15.9% of the existing Land and Building Tax potential. INDONESIAPengalihan pengelolaan Pajak Bumi Bangunan (PBB) dari pajak pusat ke daerah dimaksudkan untuk meningkatkan Pendapatan Asli Daerah (PAD). Penelitian ini bertujuan untuk mengetahui efektivitas penerimaan PBB Kota Lubuklinggau bagi peningkatan PAD Kota Lubuklinggau, serta untuk menganalisis pengelolaan PBB Kota Lubuklinggau terutama potensi penerimaan yang bersumber dari PBB. Untuk mengukur tingkat efektivitas penerimaan PBB digunakan analisis data potensi dan realisasi penerimaan PBB, sedangkan pengukuran potensi peningkatan penerimaan PBB dilakukan dengan menganalisis luas wilayah potensi pajak, objek pajak nilai jual, dan menghitung besarnya potensi penerimaan PBB berdasarkan tarif PBB dengan nilai jual objek pajak kena pajak. Hasil penelitian menunjukkan bahwa pada tahun 2017-2021, tingkat efektivitas penerimaan PBB dikategorikan tidak efektif karena persentase realisasi penerimaan PBB <60%. Tahun 2021 nilai efektivitas sebesar 39,63%. Sementara itu, potensi peningkatan pendapatan PBB cukup besar namun persentase potensi penerimaan PBB tahun 2021 hanya 15,9% dari potensi PBB yang ada, sehingga PBB di Lubuklinggau belum optimal.
Biomechanical effects of hemin and sildenafil treatments on the aortic wall of chronic-hypoxic lambs
Authors: Navarrete Álvaro , Inostroza Matías , Utrera Andrés , Bezmalinovic Alejandro , González-Candia Alejandro , Rivera Eugenio , Godoy-Guzmán Carlos , Herrera Emilio A. , García-Herrera Claudio 
Published: July 2024

Introduction: Gestation under chronic hypoxia causes pulmonary hypertension, cardiovascular remodeling, and increased aortic stiffness in the offspring. To mitigate the neonatal cardiovascular risk, pharmacological treatments (such as hemin and sildenafil) have been proposed to improve pulmonary vasodilation. However, little is known about the effects of these treatments on the aorta. Therefore, we studied the effect of hemin and sildenafil treatments in the aorta of lambs gestated and raised at highlands, thereby subjected to chronic hypoxia.Methods: Several biomechanical tests were conducted in the descending thoracic aorta (DTA) and the distal abdominal aorta (DAA), assessing 3 groups of study of hypoxic animals: non-treated (Control) and treated either with hemin or sildenafil. Based on them, the stiffness level has been quantified in both zones, along with the physiological strain in the unloaded aortic duct. Furthermore, a morphological study by histology was conducted in the DTA.Results: Biomechanical results indicate that treatments trigger an increment of axial pre-stress and circumferential residual stress levels in DTA and DAA of lambs exposed to high-altitude chronic hypoxia, which reveals a vasodilatation improvement along with an anti-hypertensive response under this characteristic environmental condition. In addition, histological findings do not reveal significant differences in either structure or microstructural content.Discussion: The biomechanics approach emerges as a valuable study perspective, providing insights to explain the physiological mechanisms of vascular function. According to established results, alterations in the function of the aortic wall may not necessarily be explained by morphostructural changes, but rather by the characteristic mechanical state of the microstructural components that are part of the studied tissue. In this sense, the reported biomechanical changes are beneficial in mitigating the adverse effects of hypobaric hypoxia exposure during gestation and early postnatal life.
Discovery and prospects of new heterocyclic Isatin-hydrazide derivative with a novel role as estrogen receptor α degrader in breast cancer cells
Authors: Arif Muhammad Nouman , Sarwar Sadia , Firdous Farhat , Saleem Rahman Shah Zaib , Nadeem Humaira , Alamro Abir Abdullah , Alghamdi Amani Ahmad , Alshammari Atekah Hazza , Farooq Omer , Khan Rashid Ali , Faisal Amir 
Published: July 2024

Introduction: Isatin, a heterocycle scaffold, is the backbone of many anticancer drugs and has previously been reported to engage multiple cellular targets and mechanisms, including angiogenesis, cell cycle, checkpoint pathways and multiple kinases. Here, we report that a novel isatin derivative, 5i, degrades estrogen receptor alpha (ERα) in estrogen-dependent breast cancer cells. This effect of the isatin nucleus has not been previously reported. Tamoxifen and fulvestrant represent standard therapy options in estrogen-mediated disease but have their own limitations. Isatin-based triple angiokinase inhibitor BIBF1120 (Nintedanib) and multikinase inhibitor Sunitinib (Sutent) have been approved by the FDA.Methods: Keeping this in view, we synthesized a series of N'-(1-benzyl-2-oxo-1, 2-dihydro-3H-indol-3-ylidene) hydrazide derivatives and evaluated them in vitro for antiproliferative activities in MCF-7 (ER+) cell line. We further investigated the effect of the most potent compound (5i) on the Erα through Western Blot Analysis. We used in silico pharmacokinetics prediction tools, particularly pkCSM tool, to assess the activity profiles of the compounds.Results and discussion: Compound 5i showed the best antiproliferative activity (IC50 value; 9.29 ± 0.97 µM) in these cells. Furthermore, 5i downregulated ERα protein levels in a dose-dependent manner in MCF-7. A multifaceted analysis of physicochemical properties through Data Warrior software revealed some prominent drug-like features of the synthesized compounds. The docking studies predicted the binding of ligands (compounds) with the target protein (ERα). Finally, molecular dynamics (MD) simulations indicated stable behavior of the protein-ligand complex between ERα and its ligand 5i. Overall, these results suggest that the new isatin derivative 5i holds promise as a new ERα degrader.
Assessment of heavy metals and its treatment through phytoremediation in groundwater along River Kabul in district Charsadda
Authors: Khan Shehryar , Kamal Masroor , Noor Shah , Afzal Syes Muhammad 
Published: July 2024

Life cannot exist without water. Water scarcity is caused by massive groundwater decline. Water contamination is the most common problem spreading worldwide quicker than ever, along with anthropogenic water scarcity. Since tainted water can harm health, water contamination is also contributing to water scarcity. Heavy metals in drinking water have plagued most Asian, African, and European nations for decades. Studies reveal that heavy metals have caused damage in Pakistan, India, and China. This study detects heavy metals in groundwater and treats them with phytoremediation along the river Kabul in district Charsadda. It also measures pH, EC, TDS, turbidity, fluoride, phosphates, nitrites, and nitrates. All indicators meet the WHO and national environmental quality criteria for drinking water, except for turbidity, which exceeds the limit of ≤5 NTU in four examined regions, reaching a maximum of 9.99 NTU. Chemical parameters were within the standard limits, except for high concentrations of arsenic (As) and cadmium (Cd) in samples from S2 (15.20 μg/L) and S1 (20.50 μg/L) compared to WHO’s 10 and 5 μg/L standards. However, the limit is within EPA Pakistan’s 50 and 100 μg/L standards for drinking water, which Pakistan still follows. Heavy metals can harm health, even at low levels. Since the majority of the study area’s population relies on groundwater for drinking and other needs, heavy metal pollution of the groundwater can cause many ailments. Thus, phytoremediation is increasingly vital to reduce these heavy metals to WHO limits to protect human health and the environment.
Vedolizumab plus basiliximab as second-line therapy for steroid-refractory lower gastrointestinal acute graft-versus-host disease
Authors: Gao Zicheng , Fan Zhiping , Liu Zhi , Ye Xu , Zeng Yunxin , Xuan Li , Huang Fen , Lin Ren , Sun Jing , Liu Qifa , Xu Na 
Published: July 2024

BackgroundSteroid-resistant (SR) lower gastrointestinal (LGI) tract graft-versus-host disease (GVHD) is the predominant cause of morbidity and mortality from GVHD after allogeneic hematopoietic stem cell transplantation (allo-HSCT). The role of vedolizumab in the treatment of SR-LGI acute GVHD (aGVHD) remains uncertain. We aimed to assess the efficacy and safety of vedolizumab combined with basiliximab as second-line therapy for SR-LGI-aGVHD.MethodsThis study aimed to explore the efficacy of vedolizumab combined with basiliximab for SR-LGI-aGVHD. The primary endpoint was the overall response (OR) on day 28. Secondary and safety endpoints included durable OR at day 56, overall survival (OS), chronic GVHD (cGVHD), non-relapse mortality (NRM), failure-free survival (FFS), and adverse events.ResultsTwenty-eight patients with SR-LGI-aGVHD were included. The median time to start of combination therapy after SR-LGI-aGVHD diagnosis was 7 (range, 4–16) days. The overall response rate (ORR) at 28 days was 75.0% (95% CI: 54.8%–88.6%), and 18 achieved a complete response (CR) (64.3%, 95% CI: 44.1%–80.7%). The durable OR at day 56 was 64.3% (95% CI: 44.1%–80.7%). The 100-day, 6-month, and 12-month OS rates for the entire cohort of patients were 60.7% (95% CI: 45.1%–81.8%), 60.7% (95% CI: 45.1%–81.8%), and 47.6% (95% CI: 31.4%–72.1%), respectively. The median failure-free survival was 276 days; (95% CI: 50–not evaluable) 12-month NRM was 42.9% (95% CI: 24.1%–60.3%). The 1-year cumulative incidence of cGVHD was 35.7%. Within 180 days after study treatments, the most common grade 3 and 4 adverse events were infections. Nine (32.1%) patients developed cytomegalovirus (CMV) reactivation complicated with bacterial infections (25.0%, CMV infection; 7.1%, CMV viremia). Epstein–Barr virus (EBV) reactivation occurred in five patients (17.9%, 95% CI: 6.8%–37.6%). Only three patients (10.7%, 95% CI: 2.8%–29.4%) in our study developed pseudomembranous colitis.ConclusionsVedolizumab plus basiliximab demonstrated efficacy in severe SR-LGI-aGVHD and was well-tolerated. Vedolizumab plus basiliximab may be considered a potential treatment option for patients with LGI-aGVHD.