bluuebunny/crossref_metadata_2025_split huggingface dataset:
Le développement professionnel perçu des animateurs d'un dispositif d'insertion en lycée agricole
Authors: Aussel Lucie 
Published: June 2013

Nous nous proposons d’appréhender le développement professionnel perçu d’employés d’une association spécialiste du recrutement et de l’insertion des cadres et techniciens du secteur agricole dans une situation innovante de travail. Ces professionnels ont été sollicités pour mettre en place une expérimentation sociale, une formation à destination d’étudiants inscrits en brevet de technicien supérieur agricole. La mise en place de cette expérimentation a entraîné une normalisation des pratiques modifiant le « travailler ensemble ». Des pratiques de type collaboratif ont émergé, les échanges entre les travailleurs ont augmenté. Dans ce contexte particulier de rupture de la routine professionnelle, nous questionnons la capacité d’adaptation de ces travailleurs. Quels effets ces changements provoquent-ils sur leur développement professionnel perçu ? Nous avons ciblé la compétence comme indicateur de développement professionnel de ces travailleurs. De plus, nous avons choisi de faire passer des entretiens d’autoconfrontation à ces professionnels. L’analyse de ces entretiens a permis de mettre au jour l’apparition de nouvelles compétences.
BONE MARROW ABONRMALITIES IN HIV INFECTION
Authors: Dhurve Sharad Antiram 
Published: June 2013

ABSTRACT Introduction; Hematological abnormalities are a common complication of HIV infection. Bone marrow abnormalities occur in all stages of HIV infection. Present work was carried out to study the bone marrow abnormalities in patients with HIV/AIDS. Methods: 160 patients of HIV +ve were included in the study. A complete blood count, relevant biochemical investigations, CD4 counts were done, besides a thorough history and clinical examination. HIV positive patients were classified as those having AIDS and those without AIDS according to NACO criteria. Bone marrow examination was performed for indication of anemia, leucopenia, pancytopenia and thrombocytopenia. Results: As per CDC criteria 59.81% patients had AIDS in 107 patients. The most common hematological abnormality was anemia, seen in 93.12% patients. Bone marrow was normocellular in 79.06% of non-AIDS and 79.68% of AIDS, hypocellular in 13.95%.Thrombocytopenia was seen in 4 cases of ART (4.93%) and 3 cases (4.68%) of AIDS group. Abnormal cells like plasma cell, histocyte and toxic granule found in bone marrow. Conclusions: Myelodysplasia was more common in AIDS than in non AIDS patients. Granulocytic series is most commonly associated with evidence of dysplasia. Anemia in HIV patients can be a good clinical indicator to predict and access the underlying immune status. Thus bone marrow study is imperative to methodically observe and follow clinical and laboratory aberration in such patients in order to improve our diagnostic and therapeutic skills pertinent to HIV/AIDS.
ASSESSMENT OF DIAGNOSTIC TECHNIQUES OF URINARY TUBERCULOSIS
Authors: Ghaleb Khaled Ismail , Afifi Magdy Mohamed , El Gohary Mohamed Mohamed 
Published: June 2013

Khalid Ghaleb a,* , Magdy Afifib, Mohamad El-Gohary c aDepartment of Medical Laboratories, Faculty of Applied Medical Science, King Khalid University, Bisha 551, Saudia Arabia bDepartment of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 71524, Egypt cDepartment of Internal Medicine, Faculty of Medicine, Al-Azhar University, Assuit, Egypt The corresponding author e-mail: kh_ghaleb4@hotmail.com Current Tel: 00966595388496 Saudia, 00201119338055 Egypt The place of the study worked : Department of Botany and Microbiology, Faculty of Science, Al-Azhar University, Assuit 71524, Egypt, e-mail: afifi_magdy@ymail.com Tel: 00201006554961 Abstract Early diagnosis of active tuberculosis remains an elusive challenge. In addition, one third of the world's population is latently infected with Mycobacterium tuberculosis (Mtb) and up to 10% of infected individuals develop tuberculosis (TB) in their lifetime. In this investigation, the incidence of urinary tuberculosis among renal patients was studied. Three hundreds urine samples were processed for detection of Mtb by Ziehl-Neelson (ZN) smear examination, Lowenstein Jensen (LJ) medium, radiometric BACTEC460 system as well as polymerase chain reaction (PCR) and DNA Enzyme Immunoassay (DEIA) test. Out of 300 urine samples, 2 were positive by both ZN smears and LJ medium with incidence rate of 0.66 %, 3 positive samples by BACTEC460 culture system with incidence of 1%. PCR assay gave more positive results than smear and culture examination (i.e. 8 positive samples with incidence rate of 2.6%). The specificities were 25% for both ZN smears and LJ medium, 37.5% for BACTEC460 culture system, and 100% for PCR test, while sensitivities of all assays were 100%. Thus PCR is a rapid and sensitive method for the early diagnosis of urinary tuberculosis. Keywords: List of abbreviations:Acid Fast Bacilli (AFB)-Base pair (bp)-DNA Enzyme Immunoassay (DEIA) -Extrapulmonary Tuberculosis (EPTB)-International Union Against Tuberculosis (IUAT)-Lowenstein Jensen (LJ)-Mycobacterium tuberculosis (Mtb) -Polymerase Chain Reaction (PCR)-Tuberculosis (TB)-Urinary Tuberculosis (UTB-Urogenital Tuberculosis (UGTB)-Ziehl-Neelson (ZN)
Changes in Anterior Segment Morphology after Intravitreal Injection of Bevacizumab and Bevacizumab–triamcinolone Acetate Combination
Authors: Alkin Zeynep , Perente Irfan , Altan Cigdem , Konstantinidis Aristeidis , Ozkaya Abdullah , Yuksel Kemal , Yazici Ahmet Taylan , Demirok Ahmet 
Published: June 2013

Purpose To evaluate the effects of 2 different amounts of commonly used intravitreal agents on anterior segment morphology in conjunction with intraocular pressure (IOP) changes. Methods Two groups of 46 patients who received intravitreal injection of bevacizumab (group 1, 0.05 mL) or bevacizumab–triamcinolone acetate (TA) combination (group 2, 0.1 mL) were included in this prospective study. All patients underwent anterior segment optical coherence tomography (AS-OCT) imaging with Visante OCT and IOP measurement before and at 5 minutes, 1 hour, and 3 hours after injection. Anterior chamber depth (ACD), angle-opening distance (AOD 500-750), trabecular–iris space area (TISA 500-750), and anterior chamber angle (ACA) were measured. Results were compared between groups and measurement time points. Results The mean change in IOP showed significant difference between baseline and first measurements after the injection in group 1 and group 2 (p = 0.012, p = 0.008, respectively). There was a statistically significant difference from baseline in ACD at the first and second measurements in group 2 (p = 0.005, p = 0.018, respectively). All angle parameters showed insignificant decrease from baseline at all measurement time points in both groups (p>0.05 for each). The difference of angle parameters after injection was not statistically significant between the groups (p>0.05 for each). Conclusions Intravitreal injection of bevacizumab (0.05 mL) or bevacizumab-TA combination (0.1 mL) caused a change in anterior segment configuration determined by AS-OCT. The changes in anterior chamber parameters returned to baseline values with the normalization of IOP values after the first few minutes of injection.
Development and Validation of a Stability-Indicating HPLC-Photodiode Array Detector Method for Formulation Analysis and Degradation Kinetics and Dissolution Studies of Mycophenolate Sodium and HPLC/MS/MS Characterization of its Stress Degradation Products
Authors: Nikalje Anna Pratima G , Choudhari Vishnu P 
Published: June 2013

Abstract
A simple stability-indicating isocratic RP-HPLC method was developed and validated for the determination of mycophenolate sodium and its alkali degradation product. Forced degradation of the drug was carried out under thermolytic, photolytic, acid/base hydrolytic, and oxidative stress conditions. Alkali degradation product DP1 was isolated, and separation of stress degradation products was achieved on a Symmetry C18 (250 × 4.6 mm × 5.0 μm) column using the mobile phase methanol–acetate buffer adjusted with acetic acid to pH 6.0 (76 + 24, v/v) at a 0.55 mL/min flow rate and 50°C. Data were integrated at the detection wavelength of 251 nm. The method validation characteristics included accuracy, precision, linearity, range, specificity, and sensitivity per International Conference on Harmonization guidelines. Robustness testing was conducted to evaluate the effect of minor changes in the chromatographic conditions and to establish appropriate system suitability parameters. Structural elucidation of degraded products was performed by HPLC/MS/MS. The method was used successfully for drug product analysis, dissolution study, and determination of the drug's acid, alkali, and oxidative degradation kinetics.
Development and Validation of an HPLC Method for Simultaneous Detection and Quantification of Paracetamol and Etodolac in Human Plasma and Its Application to a Pharmacokinetic Study
Authors: Gorain Bapi , Choudhury Hira , Nandi Utpal , Das Ayan , Dan Shubhasis , Pal Tapan K 
Published: June 2013

Abstract
A simple, reproducible, and feasible RP-HPLC method has been developed for simultaneous determination of two commonly used drugs, paracetamol (PCM) and etodolac (EDL), in human plasma. In this specific and accurate method, tinidazole was used as an internal standard. Chromatographic separation was achieved on a Hiber C18 250 × 4.6 mm id, 5 μm particle size, column using the isocratic mobile phase 10 mM potassium phosphate buffer (adjusted to pH 7.5)–acetonitrile (70 : 30, v/v) at a flow rate of 1 mL/min. Detection was at 235 nm. The linear concentration range of the assay was 0.100 to 50.00 μg/mL for both analytes, and the linear regression correlation coefficient was (r2 &gt; 0.99). The recovery of PCM and EDL were 94.03 and 88.27%, respectively. The method was validated for specificity, linearity, precision, accuracy, reproducibility, and stability. The intraday and interday precision and accuracy values for PCM and EDL met the acceptance criteria of U.S. Food and Drug Administration guidelines. PCM and EDL were stable in a battery of stability studies, viz., bench top, dry extract, and freeze-thaw cycles. This developed and validated method was successfully applied to quantitatively assess the PCM and EDL for the first time in human plasma obtained from a pharmacokinetic study performed with 12 healthy human volunteers.
Interlaboratory Proficiency Testing for Measurement of the Polycyclic Aromatic Hydrocarbon Metabolite 1-Hydroxypyrene in Fish Bile for Marine Environmental Monitoring
Authors: Kammann Ulrike , Askem Clare , Dabrowska Henryka , Grung Merete , Kirby Mark F , Koivisto Pertti , Lucas Claudia , McKenzie Margaret , Meier Sonnich , Robinson Craig , Tairova Zhanna M , Tuvikene Arvo , Vuorinen Pekka J , Strand Jakob 
Published: June 2013

Abstract
The determination of PAH metabolites in fish bile is performed to assess the PAH contamination in fish for environmental monitoring. A growing number of laboratories in Europe use this parameter for national monitoring. However, an international intercomparison between laboratories has not taken place in the last years. Therefore the determination of the PAH metabolite 1-hydroxypyrene was tested in a collaborative trial performed by 10 laboratories from eight countries. Five samples of naturally contaminated fish bile covering different concentration levels were distributed among the participants. The present study was open for different methods: GS/MS, HPLC-fluorescence (HPLC-F), fixed wavelength fluorescence (FWF), and synchronous fluorescence spectrometry (SFS), respectively. The results suggest that all four methods under investigation are suitable for screening purposes, but only three methods have produced comparable results which could be used for a common monitoring database: GC/MS, HPLC-F, and SFS (with conversion factor). Most z-scores were within the acceptance criteria of ±2. The comparability of GC/MS, HPLC-F, and SFS results should be further improved because SFS is a widely used method with a great potential for monitoring. The present study contributes to the quality assurance of results for European marine monitoring.
Procedural Revision to the AOAC Germicidal Spray Products as Disinfectants Test Method: Establishment of Minimum and Maximum Log Density Values for Test Microbes on Inoculated Carriers
Authors: Pines Rebecca M , Tomasino Stephen F , Cottrill Michele P , Hamilton Gordon C , Parker Albert E 
Published: June 2013

Abstract
The AOAC Germicidal Spray Products as Disinfectants test method (AOAC Official Method 961.02) is used to measure the efficacy of spray products on hard inanimate surfaces; however, the method does not provide procedures to determine the population of the test microbe on inoculated glass slide carriers (e.g., carrier counts reported as CFU/carrier). Without a method to measure and monitor carrier counts, the associated efficacy data may not be reliable and repeatable. This report provides a standardized procedure to address this issue and, based on carrier count data collected by four laboratories from 2000 to 2010, proposes a specific range for the mean log density per carrier as a requirement. Laboratory-based carrier count data were collected concurrently with 116 Method 961.02 efficacy tests conducted on spray products bearing claims against Pseudomonas aeruginosa and Staphylococcus aureus. For many of the tests a soil load (SL) was added to the inoculum (as specified on the product label claim). Six carriers were assayed per test for a total of 696 carriers. All but two of the 116 mean log densities were at least 5.0 (a geometric mean of 1.0 × 105 CFU/carrier). Across the four combinations of microbes and SL treatments, the mean TestLD (mean log density across all enumerated carriers in a test) ranged from approximately 6.0 (a geometric mean of 0.9 × 106 CFU/carrier) to 6.3 (a geometric mean of 2.0 × 106 CFU/carrier). Across all microbes and SL treatments, the mean log density (±SEM) was 6.2 (±0.07) per carrier (a geometric mean of 1.5 × 106 CFU/carrier). The mean log density for six carriers per test showed good repeatability (0.32) and reproducibility (0.34). The proposed requirement for S. aureus tests and P. aeruginosa tests is a mean log density (across six carriers) between 5.0 and 6.5. A separate 2009 study at three laboratories was conducted to evaluate the persistence of P. aeruginosa, S. aureus, and Salmonella enterica on glass carriers. Based on the persistence data, a 2 h use period is proposed for using the inoculated carriers post drying. The persistence data set was also used to assess the carrier counts for S. enterica. The carrier counts were approximately one log lower for S. enterica compared to S. aureus and P. aeruginosa; a range of 4.0 to 5.5 logs is proposed as a requirement for S. enterica tests.
iQ-Check Listeria monocytogenes II
Authors: Lauer Wendy F , Tourniaire Jean-Philippe 
Published: June 2013

Abstract
A comparative evaluation study of the Bio-Rad® iQ-Check™Listeria monocytogenes II Kit (Bio-Rad Laboratories, Hercules, CA) was conducted at Q Laboratories, Inc., Cincinnati, OH. iQ-Check is a rapid method based on real-time PCR amplification and detection of L. monocytogenes in food and environmental samples. The iQ-Check method was compared to the Health Canada MFHPB-30 reference method for the analysis of five ready-to-eat meats—deli turkey, hot dogs, liver paté, raw fermented sausage, and deli ham—and one stainless steel surface. Each food matrix was analyzed at two contamination levels: a low level at 0.2–2 CFU/25 g and a high level at 2–5 CFU/25 g. The environmental surfaces were analyzed at a low level of 0.2–2 CFU/5 cm2 sampling area and a high level of 2–5 CFU/5 cm2 sampling area. There were 20 replicates per contamination level and five control replicates at 0 CFU/25 g or 0 CFU/5 cm2 sampling area (uninoculated). All samples detected by iQ-Check were subsequently confirmed by reference method protocol. There was no significant difference in the number of positive samples detected by the iQ-Check Listeria monocytogenes II Kit in comparison to the Health Canada MFHPB-30 method for all matrixes tested.
Chemoenzymatic Site‐Specific Reversible Immobilization and Labeling of Proteins from Crude Cellular Extract Without Prior Purification Using Oxime and Hydrazine Ligation
Authors: Mahmoodi Mohammad M. , Rashidian Mohammad , Dozier Jonathan K. , Distefano Mark D. 
Published: June 2013

AbstractIn a facile and potentially general method for protein modification at the C‐terminus, aldehyde‐modified proteins, obtained from enzymatic protein prenylation, react rapidly with hydrazide and aminooxy surfaces and fluorophores at neutral pH and in micromolar concentration ranges of reagents. This strategy was used for fluorescent labeling of eGFP‐CVIA, as a model protein, with aminooxy and hydrazide fluorophores or PEGs, and immobilization onto and subsequent release of the protein from hydrazide‐functionalized agarose beads using hydrazone‐oxime exchange. This method is described in detail here and provides site‐specifically PEGylated or fluorescently labeled proteins starting from crude cellular extract in three steps: prenylation, capture, and release. Curr. Protoc. Chem. Biol. 5:89‐109 © 2013 by John Wiley & Sons, Inc.
Tuning Nanoparticle Catalysis for the Oxygen Reduction Reaction
Authors: Guo Shaojun , Zhang Sen , Sun Shouheng 
Published: June 2013

AbstractAdvances in chemical syntheses have led to the formation of various kinds of nanoparticles (NPs) with more rational control of size, shape, composition, structure and catalysis. This review highlights recent efforts in the development of Pt and non‐Pt based NPs into advanced nanocatalysts for efficient oxygen reduction reaction (ORR) under fuel‐cell reaction conditions. It first outlines the shape controlled synthesis of Pt NPs and their shape‐dependent ORR. Then it summarizes the studies of alloy and core–shell NPs with controlled electronic (alloying) and strain (geometric) effects for tuning ORR catalysis. It further provides a brief overview of ORR catalytic enhancement with Pt‐based NPs supported on graphene and coated with an ionic liquid. The review finally introduces some non‐Pt NPs as a new generation of catalysts for ORR. The reported new syntheses with NP parameter‐tuning capability should pave the way for future development of highly efficient catalysts for applications in fuel cells, metal‐air batteries, and even in other important chemical reactions.
Reconstitution of a cytokine receptor scaffold utilizing multiple different tyrosine motifs
Authors: Saka Koichiro , Kawahara Masahiro , Nagamune Teruyuki 
Published: June 2013

ABSTRACTCells have intracellular signal transduction systems to control cellular fates. Cytokine receptors initiate the intracellular signaling by recruiting specific signaling molecules to a range of tyrosine‐containing motifs. To specifically activate a target signaling molecule, we previously designed single‐chain Fv/cytokine receptor chimeras incorporating single tyrosine motifs in the intracellular domain, and each chimeric receptor activated the corresponding signaling molecule by oligomeric antigen stimulation. However, synergistic effects of multiple signaling pathways are indispensable to regulate complex cellular fates. In this study, we extended our approach by incorporating two different motifs in the chimeric receptor, which would result in the activation of multiple signaling molecules. We used retroviral transduction to express chimeric receptors in a murine interleukin‐3‐dependent pro‐B cell line, Ba/F3. Our results indicate that the chimeric receptors incorporating two different motifs can activate both corresponding signaling molecules by the ligand stimulation, and that the signaling intensities are influenced by the distance between two motifs. Moreover, these chimeric receptors transduced downstream signaling, which exerted synergistic effects on cellular proliferation. Our system may be used for efficiently controlling fates of various types of cells, which will be applied to tissue engineering. Biotechnol. Bioeng. 2013;110: 3197–3204. © 2013 Wiley Periodicals, Inc.
New Insights into the Pharmacological Chaperone Activity of C2‐Substituted Glucoimidazoles for the Treatment of Gaucher Disease
Authors: Li Zhonghua , Li Tiehai , Dai Shaoxing , Xie Xiaoli , Ma Xiaofeng , Zhao Wei , Zhang Weimin , Li Jing , Wang Peng George 
Published: June 2013

AbstractMutations in acid β‐glucocerebrosidase (GCase) lead to the accumulation of the sphingolipid glucosylceramide, thereby resulting in Gaucher disease (GD). Active‐site‐specific competitive GCase inhibitors are effective pharmacological chaperones (PCs) that act as folding agents for mutant GCase folding in the endoplasmic reticulum. In this study, we prepared a series of glucoimidazole C2‐substituent derivatives, and evaluated their inhibition and PC properties with GCase. A cell‐based assay with patient‐derived lymphoblasts (N370S or L444P mutations) demonstrated that administration of these compounds can significantly increase GCase activity. Interestingly, the 3,3‐dimethyl‐N‐phenyl‐4‐amide‐1‐butyl‐substituted moderate inhibitor 11 had the greatest effect on activity: 2.1‐fold increase in N370S lymphoblasts at 2.5 μM and 1.2‐fold increase in L444P at 0.5 μM following a three‐day incubation. Computer docking studies and a protease protection assay were used to elucidate the ligand–enzyme interactions responsible for the chaperone activity of 11. Western blot and immuno‐fluorescence assays verified restoration of GCase trafficking to the lysosome. Together, these results indicate that 11 is a promising PC for GD treatment and provide direct evidence of the mechanism of GCase chaperoning.
Enhanced Performance of Solution‐Processed TESPE‐ADT Thin‐Film Transistors
Authors: Chen Liang‐Hsiang , Hu Tarng‐Shiang , Huang Peng‐Yi , Kim Choongik , Yang Ching‐Hao , Wang Juin‐Jie , Yan Jing‐Yi , Ho Jia‐Chong , Lee Cheng‐Chung , Chen Ming‐Chou 
Published: June 2013

AbstractA solution‐processed anthradithiophene derivative, 5,11‐bis(4‐triethylsilylphenylethynyl)anthradithiophene (TESPE‐ADT), is studied for use as the semiconducting material in thin‐film transistors (TFTs). To enhance the electrical performance of the devices, two different kinds of solution processing (spin‐coating and drop‐casting) on various gate dielectrics as well as additional post‐treatment are employed on thin films of TESPE‐ADT, and p‐channel OTFT transport with hole mobilities as high as ∼0.12 cm2 V−1 s−1 are achieved. The film morphologies and formed microstructures of the semiconductor films are characterized in terms of film processing conditions and are correlated with variations in device performance.