bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin Typing, Sun Exposure, and Sunscreen Use in a Population of Japanese
Authors: Kawada Akira , Hiruma Masataro , Noda Toshiaki , Kukita Atsushi 
Published: April 2015

AbstractDistribution of Japanese skin types (JSTs), sun‐exposure habits, and sunscreen use were surveyed in 379 new outpatients. The largest number of subjects belonged to JST class J‐II (65%), J‐I was second (24%), and J‐III was third (11%). Eighty‐eight percent of those studied had occasional or habitual sun exposure during the summer, and many were exposed for more than three hours per exposure day (80% of those with occasional exposure; 62% of those with habitual exposure). More younger persons had occasional sun exposure than older persons. More males had habitual sun exposure than females. Sunscreens were used by 41% of the subjects, but 77% of these used them without accurate understanding of the definition of the sun protection factor. More J‐I subjects used sunscreens than J‐II and J‐III subjects. More females than males used them.
Electron Microscopic Study of the Colloid‐like Substance in Solar Elastosis
Authors: Matsuta Mayumi , Kunimoto Mitsuo , Kosegawa Gen , Akasaka Toshihide , Kon Saiichi 
Published: April 2015

AbstractWe have made electron microscopic studies on the elastotic material of solar elastosis which developed in cutis rhomboidalis nuchae taken from 10 males, 50–83 years of age. It was revealed that this material of the cutis rhomboidalis nuchae contained a colloid‐like substance consisting of both a fine granular and an amorphous component. The colloid‐like substance closely resembled that which is seen in the adult‐type colloid milium, and could not be distinguished from it under light microscopy.In view of the morphological similarity at the ultrastructural level between the elastotic material and the colloid‐like substance, it was proposed that normal elastic fibers can change into elastotic material and then further degenerate into the colloid‐like substance, eventually becoming the typical colloid substance seen in colloid milium.
Prolyl Hydroxylase and Procollagen III Peptide Levels in the Sera from Patients with Collagen Diseases and Psoriasis
Authors: Sasaki Tetsuo , Nakajima Hiroshi 
Published: April 2015

AbstractProlyl hydroxylase (PH) and procollagen III peptide (PIIIP) levels in 43 serum samples from various collagen diseases including 19 progressive systemic sclerosis (PSS) cases were simultaneously assayed and compared to those of 22 age‐matched psoriasis patients and 20 healthy controls in order to examine whether they can serve as useful parameters of fibrotic changes, especially in the skin. Serum PH levels were determined with enzyme immunoassay and serum PIIIP levels with radioimmunoassay. The average values of PH were 109% (n=3, p<0.001; generalized morphea, GM), 104% (n=1; overlap syndrome, OS), 57% (n=6; mixed connective tissue disease, MCTD), 48% (p<0.05; PSS), 33% (n=5; dermatomyositis, DM), 28% (psoriasis), and 9% (n=9; systemic lupus erythematosus, SLE), higher than the control levels, while those of PIIIP were 82% (OS), 57% (MCTD), 42% (p<0.01; PSS), 11% (GM), and 5% (DM) higher and 22% (psoriasis) and 28% (SLE), lower than the controls. These results indicate that both values were higher in the diseases with skin fibrosis and lower in those without it, suggesting that both assays generally reflect the state of actual progression of fibrosis at the point of determination. These two values had considerable correlation; however, GM, psoriasis and SLE patients had relatively higher values of PH than of PIIIP. This result suggests that the PH assay may also reflect the state of connective tissue activation; therefore the PIIIP assay may be more specific for pathological fibrotic process. Thus it seems that these two assays can serve as parameters of the activity of fibrotic skin diseases, especially when assayed simultaneously and regularly.
Clinical Observations of Mosquito Bite Reactions in Man: A Survey of the Relationship between Age and Bite Reaction
Authors: Oka Keiko , Ohtaki Noriko 
Published: April 2015

AbstractTo evaluate the mechanism of mosquito bite reaction in man, the reaction to Aedes albopictus was observed in 162 subjects ranging in age between 1 to 68 years old. Bite reactions were found to consist of both an immediate and a delayed reaction. The eruption and time course of the immediate reaction were consistent with type I hypersensitivity. The eruption and time course of the delayed reaction were consistent with cutaneous basophil hypersensitivity. Positive rates of immediate reaction increased from early childhood to adolescence and decreased with age from adulthood. The appearance and intensity of the delayed reaction decreased with age. Mosquito bite reactions in human beings exposed continuously and regularly are known to change from stage 1 to stage 5 (stage 1; no reaction, 2; delayed reaction only, 3; immediate and delayed reaction, 4; immediate reaction only, 5; no reaction). Analysis of the relationship between age and bite reaction in this study indicated that the principle held true even when the exposures were irregular or at random.
Drug‐induced Chronic Pigmented Purpura
Authors: Nishioka Kiyoshi , Katayama Ichiro , Masuzawa Mikio , Yokozeki Hiroo , Nishiyama Shigeo 
Published: April 2015

AbstractA close correlation between purpuric reaction and drugs was observed in seven cases of chronic pigmented purpura. The patients developed purpuric lesions after taking certain drugs for more than 3 years, were thiamine propyldisulfide in 2 cases, and chlordiazepoxide in 1 case. The purpuric lesions stopped recurring after removal of the drugs in the rest of the cases. It is suggested that drugs are among the etiological factors in chronic pigmented purpura.
Paget's Disease of the Male Breast
Authors: Sano Yoshimi , Inoue Tadanori , Aso Miki , Hagari Yoshitaka , Hattori Kyoko , Shimao Shuhei , Notsu Takeshi 
Published: April 2015

AbstractWe report a 61‐year‐old male with mammary Paget's disease. Physical examination revealed a slightly exudative erythema at the areola and a reddish, enlarged left nipple. No tumor or left axillary lymph nodes was palpable. He underwent a left modified radical mastectomy. Histologically, there was an intraductal carcinoma in the upper portion of the mammary ducts. The axillary lymph nodes that were examined were free of metastasis. Paget cells had neither estrogen nor progesterone receptors. We speculated that the histogenesis of Paget cells involved carcinoma cells that invaded the epidermis of the nipple.
Delayed Cutaneous Metastasis of Gastric Carcinoma
Authors: Fujiwara Sakuhei , Ichikawa Hiroki , Matsunaga Etsuji , Shinkai Hiroshi , Takayasu Susumu , Wada Koichi , Sekine Hiroshi 
Published: April 2015

AbstractThis paper describes a case of skin cancer resembling carcinoma en cuirasse associated with multiple bone lesions, which were both judged to be due to metastasis of poorly differentiated carcinoma of the stomach, the primary lesion excised ten years previously. Overt bone involvement had been observed for more than two years and a half. Histologic examination of a skin biopsy specimen showed that carcinoma cells had infiltrated into the middle and lower dermis, forming clusters and strands in an abundunce of fibrous stromata. No primary lesion was detected in the remnant stomach at autopsy.
Bacterial Gangrene on the Cheek of a Comatose Patient
Authors: Kikuchi Ichiro , Sakaguchi Ei 
Published: April 2015

AbstractA 68‐year‐old Japanese woman with sepsis developed a gangrene on her left cheek from a small wound in contact with a gastric tube. Klebsiella pneumoniae was cultured from the sputum, wound, and the blood and was assumed to be responsible for this condition, possibly through thrombosis of an artery.
Langerhans Cells of Human Mucosa
Authors: de Fraissinette Anne , Schmitt Daniel , Thivolet Jean 
Published: April 2015

AbstractThe ontogeny of human LC and their presence in all Malpighian epithelia underline their important role in immunoregulation of the skin and mucous membranes.LC are also found in buccal and esophageal mucosa, in cornea and conjunctiva, in pulmonary, vesical, vaginal and cervical epithelia as well as in placenta villi. In all these Malpighian epithelia, the presence of DR+ LC is necessary and essential for the surveillance function against allergoantigens and the emergence of neo‐antigens associated with malignant transformations.
Abnormal Division and Gene Expression in Cultured Cells from a Patient with Tuberous Sclerosis
Authors: Onodera Kazukiyo , Ishibashi Yasumasa , Sasaki Masafumi , Kimura Genki 
Published: April 2015

AbstractTuberous sclerosis is a dominant hereditary disease characterized by the appearance of angiofibromas on the face, epileptic attacks, and mental retardation. Attention has been paid to the atypical cells observed histologically in the stroma of the angiofibroma as they may play an important role in forming lesions. We have established a cell line from an angiofibroma to clarify the nature of these cells at the cellular and biochemical levels.The cultured cells have been stably maintained for four years and show an abnormal morphology resembling that of neuronal cells. In culture, these cells divide into daughter cells, which consist of both normal cells and abnormal ones whose nuclei disintegrate and cannot divide further.The cells resemble normal fibroblasts in their pattern of staining with antibodies against tubulin, actin, vimentin, and fibronectin. However, they also stain with an antibody against the glia‐specific acidic protein, which is thought to be a specific marker protein for glial cells.The gel profile of cyclic‐AMP binding proteins was not fibroblastic, but rather of the neuron cell type. These results indicate the gene expression of these cells is also abnormal. They are a useful tool for understanding this specific genetic disease.
Superoxide Anion in Polymorphonuclear Leukocytes of Hairless Rats Suffering from Magnesium‐deficiency Dermatitis
Authors: Hanada Katsumi , Hashimoto Isao 
Published: April 2015

AbstractClinical and histological studies were made on magnesium‐deficiency dermatitis produced on a new strain of hairless rats. Superoxide anion generation in polymorphonuclear leukocytes (PMNs) and the inhibitory effect of some antioxidants and anti‐inflammatory agents against the elicited dermatitis were also examined. From the detection of increased superoxide production and effective actions of inhibitors including superoxide dismutase, cepharanthine, 4‐4'‐diamonodiphenylsulphone and 5,4,11,14‐eicosatetraynoic acid (ETYA), it is indicated that superoxide anion or related activated oxygen may be responsible for magnesium‐deficiency dermatitis as an inflammatory mediator and that the dermatitis may be related to lipoxygenase metabolites derived from arachidonic acid.
Partial Purification and Some Characteristics of Proteinase(s) Induced by Staphylococcal Exfoliative Toxin
Authors: Suzuki Kumiko , Teramoto Teruyo , Tazawa Mari , Watanabe Hideyoshi , Takiuchi Iwao 
Published: April 2015

AbstractAfter 8 hrs incubation with epidermis of newborn mice and exfoliative toxin, a marked increase in caseinolytic activity was detected, which reached a maximum at 12 hrs. Casein‐hydrolyzing enzyme(s) induced by ET were partially purified by chromatography. A substantial increase in caseinolytic activity was detected in the fractions obtained from Sephadex G‐50, while practically no caseinolytic activity was observed when the extract obtained from the epidermis incubated without ET was applied. The caseinolytic activity appeared as a single peak eluted from DEAE‐Sepharose CL‐6B and Sephadex G‐75. However, on SDS‐PAGE, the partially purified fractions exhibited several protein bands. The molecular weights of these band were estimated as 78 KD, 68 KD, 45 KD, 14.5 KD and 8.8 KD. When the partially purified enzyme(s) was preincubated with EGTA or EDTA, substantial inhibition of the activity was observed; however, no recovery of the activity was detected after the addition of CaCl2. Treatment of the enzyme(s) with PMSF and NEM caused little inactivation of the activity. Enzyme activity retained about 57% of the initial activity following 3 min incubation at 60°C, but was completely inactivated after 4 min.
Light Microscopic and Transmission Electron Microscopic Investigations of the Stratum Corneum of Xerosis Vulgaris of the Leg
Authors: Hori Makoto , Kligman Albert M. 
Published: April 2015

AbstractHistopathological findings from eight cases of xerosis vulgaris of the leg (XVL) were compared to those from nine normal controls using light microscopy and one micron epoxy resin‐embedded sections stained with toluidine blue and basic fuchsin (T‐F) and electron microscopy with ultra‐thin sections.Three sub‐layers were identified in the stratum corneum of all cases of XVL and normal controls by differences in color in T‐F stained specimens and by differences in density in electron microscopic findings.Electron microscopically, the intermediate sub‐layer within the three‐layered structure showed an uneven density with many empty spaces in both normal controls and cases of XVL. Statistically, only the intermediate sub‐layers of XVL increased significantly in number (p<0.05) as well as in thickness (p<0.001), compared to normal controls.These findings suggest that the intermediate sub‐layers play an important role in holding water in the skin.
Apocrine Hidrocystoma
Authors: Fukuda Michio , Kato Haruhisa , Hamada Toshio 
Published: April 2015

AbstractFive cases of apocrine hidrocystoma are reported. One is multiple and the others are solitary lesions. There are more than 60 cases reported in the Japanese literature. Apocrine hidrocystomas usually occur as a solitary lesion. Only two cases of multiple apocrine hidrocystoma have been described so far in Japan. We reviewed the literature and discussed the clinicopathologic nature of this tumor. Apocrine hidrocystomas are usually composed of a cystic cavity lined by columnar epithelium, showing apocrine type secretion. Peripheral to this, myoepithelial cells are usually seen; however, they were absent from all of the present cases. Change in hue of the tumor is another characteristic feature. About half of the reported Japanese cases exhibited distinctive coloration, variously light brown, red‐brown, or bluish. In contrast, non‐Japanese cases tended to have darker coloration.
Cutaneous Sarcoidosis Showing Multiple Papular Eruptions with Keratotic Plugs
Authors: Hagari Yoshitaka , Kambe Naoto , Shimao Shuhei , Sutoh Yuji , Ikeda Hiroshi 
Published: April 2015

AbstractA 26‐year‐old woman developed 249 discrete papules on her lower extremities. Twenty‐three of them had keratotic plugs. Histologic examination demonstrated granulomatous foci consisting of epithelioid cells and a epidermal invagination which was disrupted at the base and lateral sites. This invagination was considered to represent transepithelial elimination.
Pigmented Basal Cell Carcinoma Developing on the Lower Extremities
Authors: Ono Tomomichi , Egawa Kiyofumi , Yamamoto Shinji , Arao Tatsuyoshi 
Published: April 2015

AbstractThree cases of pigmented basal cell carcinoma were reported which needed to be differentiated from malignant melanoma based on their location and clinical features. Clinicopathologically, they were characterized by a large number of melanophages in the stroma, blockade melanocytes in the parenchyma, and phagocytosis of melanosomes in tumor cells as well as a large number of intraparenchymal melanophages. Two cases were characterized by the aggregation of melanophages in cystic spaces caused by stellate atrophy.
Lichenoid Drug Eruptions due to Nandrolone Furylpropionate (Demelon)®
Authors: Aihara Michiko , Kitamura Kazuko , Ikezawa Zenro 
Published: April 2015

AbstractA case of lichenoid drug eruption due to nandrolone furylpropionate (Demelon®) is reported. A 71‐year‐old man with aplastic anemia developed widespread erythema and reticulation with violaceous papules and pigmentation after receiving intramuscular injections of Demelon weekly for 2 months. The eruption gradually resolved after drug withdrawal, leaving reticular pigmentation. A biopsy specimen of the skin lesion showed lichenoid‐type reactions including hydropic or colloid degeneration of the basal cells, sometimes with satellite necrosis, and a band‐like subepidermal lymphocytic infiltrate. Most of the mononuclear cells stained strongly for helper/inducer T lymphocytes (Leu 3a).
Steroid‐withdrawal Rosacea‐like Dermatitis
Authors: Tomita Yasushi , Tagami Hachiro 
Published: April 2015

AbstractA patient with malignant lymphoma repeatedly developed transient rosacea‐like dermatitis several days after each interruption of continuous oral prednisone intake. We thought that the eruption was provoked by withdrawal of orally administered steroid, and thus we diagnosed the patient as having steroid‐withdrawal rosacea‐like dermatitis, one manifestation of steroid‐withdrawal syndrome.
Absence of Minocycline in Sebum?
Authors: Aubin Francois , Blanc Dominique , Guinchard Christiane , Agache Pierre 
Published: April 2015

AbstractThe authors studied the bioavailability of minocycline in sebum and serum. Blood and sebum samples were collected weekly for 6 weeks from ten healthy volunteers taking 200 mg of minocycline every day for 4 weeks. Sebum was collected by direct extraction with petroleum ether from the forehead. After evaporation, sebum was weighed on a scale accurate to 10 μg. Determination of minocycline in serum and sebum was performed using a high performance liquid chromatography technique (HPLC), with a better detection limit at 352 nm than at 400 nm (20 ng/ml and 0.324 μg/ml respectively). Our results contrast with other studies since no minocycline was detected in the sebum samples of treated subjects and microbiological assays of minocycline in sebum were also negative. In our opinion, the current hypothesis claiming that the effectiveness of minocycline in the treatment of acne vulgaris is based on sebaceous secretion should be reconsidered.
Pustular Dermatosis of the Scalp Associated with Autoimmune Diseases
Authors: Watanabe Shinichi , Takizawa Kiyohiro , Hashimoto Naoaki , Ishibashi Yasumasa 
Published: April 2015

AbstractA 36‐year‐old woman visited our hospital with a five month history of persistent pustulation, crusting, and alopecia on the vertex of the scalp. No pathological organisms were isolated from the lesions. Histological examination revealed non‐specific changes of chronic inflammation with destroyed follicles. Antibiotic therapy produced no response, but steroid therapy was effective. From these observations, a diagnosis of erosive pustular dermatosis of the scalp (EPDS), as described by Pye et al., was made. The patient also had Hashimoto's thyroiditis, autoimmune hepatitis, and Takayasu's aortitis. The laboratory studies revealed an increased erythrocyte sedimentation rate, C‐reactive protein 3+, hypergammaglobulinemia, and various auto‐antibodies, suggesting the possibility of a pathogenesis common to both this dermatosis and the autoimmune diseases.
Eosinophil‐infiltrating Folliculitis in Childhood
Authors: Dekio Satoshi , Jidoi Joji , Kawasaki Yohji 
Published: April 2015

AbstractA 5‐year‐old Japanese girl developed follicular pustules with erythematous plaques on the lower extremities. The histopathology was compatible with eosinophilic pustular folliculitis (EPF). However, the clinical findings were not typical of EPF; the pustules did not appear in crops, the pustules became ulcers, and the skin lesions appeared exclusively on non‐seborrheic areas.
A Case of Solitary Mastocytoma Suggesting a Divided Form of Mast Cell Nevus
Authors: Niizawa Midori , Masahashi Toshiko , Maie Okitaka , Takahashi Shin‐ya 
Published: April 2015

AbstractThe case of a 10‐week‐old boy with a solitary mastocytoma is reported. The lesion, noticed at birth, was located in an area extending from the dorsum of the left hand to the proximal phalanx of the ring and little fingers. It appeared as a coin‐sized, flattened dome‐shaped, round tumor with occasional blistering. The web between the two fingers was free of cutaneous changes. The diagnosis was confirmed by the histological characteristics: a densely mastocytic infiltration into the dermis. The present case of solitary mastocytoma, referred to as “mast cell nevus”, is unique because of its previously undescribed divided form.
The Subbasement Membrane Distribution of Type IV Collagen in Normal Human Skin
Authors: Kobayashi Hiromi , Ishii Masamitsu , Chanoki Miyako , Fukai Kazuyoshi , Hamada Toshio , Muragaki Yasuteru , Ooshima Akira 
Published: April 2015

AbstractSamples of normal human skin were obtained from 48 sites in 26 subjects ranging in age from 2 to 85 years. The samples were examined by indirect immunofluorescence and immunoelectron microscopy using anti‐human type IV collagen antibodies produced by immunizing rabbits with type IV collagen extracted from human placenta. Fluorescence was observed as granular or fine fibrous patterns, not only in the basement membrane at the dermo‐epidermal junction, around the vessels, and the accessory organs of the skin, but also in the dermal regions in the vicinity of the basement membranes. This suggests the presence of type IV collagen in the dermis deep to the basement membrane. Ultrastructurally, the extrabasal lamina distribution of type IV collagen was noted as a partial distribution around the fibroblasts that existed close to the basal lamina. These findings are considered to be important in examining the function of this collagen in the dermis and the dynamics and metabolism of the basement membrane under normal and abnormal conditions.
Detection of Calcium Binding Sites in Merkel Cell Granules of Fetal Rat Skin
Authors: Nindl Margit , Nakagawa Hidemi , Ishibashi Yasumasa 
Published: April 2015

AbstractWe utilized a cytochemical calcium‐staining technique to detect a potential calcium binding site within Merkel cell granules. Calcium has been shown to be essential for exocytotic release in various neuroendocrine cells and recent investigations strongly support the hypothesis that Merkel cells should be considered to be neuroendocrine cells which release their specific granules as a part of their physiological functions.After the addition of 50mM CaCl2 to glutaraldehyde as the primary fixative, buffer, and OsO4 as a postfixative, electron‐dense particles with a characteristic staining pattern were found within many MCGs. Successful removal of these particles after incubation of sections in a solution containing EDTA indicated that these particles were formed by calcium deposits.The occurrence of calcium binding structures within MCGs may imply that they play a role in the process of granule release and/or granule maturation.
A Study of Mosquito Salivary Gland Components and Their Effects on Man
Authors: Oka Keiko , Ohtaki Noriko , Yasuhara Tadashi , Nakajima Terumi 
Published: April 2015

AbstractTo evaluate the mechanism of mosquito bite reaction in man, salivary gland extracts from female Aedes albopictus were prepared. When the extract of the gland was analysed by ion‐exchange high performance liquid chromatography the amount of histamine in a pair of salivary glands of one mosquito was found to be below the limit of detection, an amount which is insufficient to produce an immediate reaction. Salivary gland extracts were fractionated to higher and lower molecular weight components. Intradermal injection tests with salivary gland extracts, which contained less than 100 ng of protein showed that the higher molecular weight fraction (molecular weight >10,000) elicited an immediate and a delayed reaction similar to a bite reaction.