bluuebunny/crossref_metadata_2025_split huggingface dataset:
Large serpiginous thrombus straddling the patent foramen ovale and traversing through mitral and tricuspid valves into both ventricles: a therapeutic dilemma of impending paradoxical embolism and recurrent pulmonary embolism.
Authors: Hamirani Yasmin Shamshuddin , Hnatiuk Oleh , Pett Stuart , Roldan Carlos 
Published: August 2014

A 51-year-old male with history of resected renal cell carcinoma and prior pulmonary embolism presented with tachypnea, tachycardia and progressive dyspnea on exertion. Chest computed tomography revealed bilateral acute pulmonary embolism. Transthoracic echocardiogram showed severe pulmonary hypertension with severe cor-pulmonale and presence of a large worm-like thrombus extending across the foramen ovale, entering both ventricles through the mitral and tricuspid valves. The risks of anti-coagulation, pharmacologic thrombolysis, and surgical thrombectomy, in a hemodynamically stable patient, posed a significant therapeutic dilemma. Ultimately, a collective decision was made to start anticoagulation, without incident. At 1 month follow up, complete resolution of the intracardiac thrombus, pulmonary hypertension, and cor-pulmonale were observed with full clinical recovery of the patient.
Quels objets numériques pour accompagner la conception dans des projets d’innovation ?
Authors: Verchère Céline , Collet Laurent 
Published: August 2014

Depuis quelques années, les grandes entreprises, notamment celles qui sont engagées dans le numérique, ont renouvelé leur manière de “faire de l’innovation” en prenant en considération les usagers, mais aussi en faisant appel à des compétences nouvelles (sociologie, design, ergonomie). Ce “tournant praticien” n’est pas sans effet sur les pratiques des chercheurs « embarqués” dans ces processus. Notre équipe de recherche s’est penchée sur la question de la représentation et de la circulation de la connaissance dans les projets de conception d’innovations. Il s’agit d’interroger en quoi les différents supports multimedia utilisés peuvent faciliter la formation, la collecte et la transmission de connaissances entre pairs, l’interdisciplinarité, voire la prise en compte de la parole “d’usagers”. Les premiers retours montrent que cette démarche amène les chercheurs à un double travail de création : sur le contenu, et sur la manipulation des supports numériques. Ils font aussi état des difficultés de maîtrise de tels supports. Ainsi, la compétence sémiotique s’avère primordiale afin de penser l’écriture propre à ces supports : la « convergence disciplinaire » devient de fait une nécessité.
L’ameublement et le décor intérieur du salon de Mars (1673-1789)
Authors: Taxil Gaëlle 
Published: August 2014

Lorsque le roi et son gouvernement s’installent durablement à Versailles à l’automne 1682, le salon de Mars est la salle des gardes, première pièce de l’appartement du Roi. Alors que Louis XIV n’occupe plus son Grand Appartement et que celui-ci devient un appartement de parade, le salon de Mars, par ses belles dimensions, se voit adjoindre deux tribunes pour les musiciens. La pièce dénommée alors « Salle de bal » dans les inventaires va jouer un rôle central dans le Grand Appartement pendant plus d’un siècle. Quel usage en fait-on ? Quels événements marquants s’y déroulent ? C’est ce que cette étude tente de mettre en lumière dans un premier temps. Le caractère festif du lieu éclaire ainsi d’un autre jour les choix décoratifs qui y furent faits. L’étude des inventaires, du Journal du Garde-Meuble de la Couronne, complétée des descriptions des mémorialistes, permet alors d’avancer des suppositions d’agencement et de distribution des meubles d’étoffes, lumières, tables, guéridons et autres objets décoratifs. Quelques-unes des plus belles pièces du mobilier d’argent y resplendissaient, situant le salon de Mars juste derrière celui d’Apollon dans la hiérarchie du décor. Après la fonte de celui-ci, les brocarts d’or et d’argent et l’éclat du mobilier de bois doré se substituèrent aux reflets de l’argent pour toujours mieux célébrer la pompe et le faste du lieu. C’est dans le respect de cette tradition que Louis XV puis Louis XVI continuèrent à entretenir ici la mémoire de leur aïeul.
Thermodynamic Analysis of Weak Protein Interactions Using Sedimentation Equilibrium
Authors: Sergeev Yuri V. , Dolinska Monika B. , Wingfield Paul T. 
Published: August 2014

AbstractProteins self‐associate to form dimers and tetramers. Purified proteins are used to study the thermodynamics of protein interactions using the analytical ultracentrifuge. In this approach, monomer‐dimer equilibrium constants are directly measured at various temperatures. Data analysis is used to derive thermodynamic parameters, such as Gibbs free energy, enthalpy, and entropy, which can predict which major forces are involved in protein association. Curr. Protoc. Protein Sci. 77:20.13.1‐20.13.15. © 2014 by John Wiley & Sons, Inc.
Isolation and Characterization of Mouse Innate Lymphoid Cells
Authors: Halim Timotheus Y.F. , Takei Fumio 
Published: August 2014

AbstractInnate lymphoid cells (ILCs) are rare populations of cytokine‐producing lymphocytes and are divided into three groups, namely ILC1, ILC2, and ILC3, based on the cytokines that they produce. They comprise less than 1% of lymphocytes in mucosal tissues and express no unique cell surface markers. Therefore, they can only be identified by combinations of multiple cell surface markers and further characterized by cytokine production in vitro. Thus, multicolor flow cytometry is the only reliable method to purify and characterize ILCs. Here we describe the methods for cell preparation, flow cytometric analysis, and purification of murine ILC2 and ILC3. Curr. Protoc. Immunol. 106:3.25.1‐3.25.13. © 2014 by John Wiley & Sons, Inc.
Measurement of Peptide Binding to MHC Class II Molecules by Fluorescence Polarization
Authors: Yin Liusong , Stern Lawrence J. 
Published: August 2014

AbstractPeptide binding to major histocompatibility complex class II (MHCII) molecules is a key process in antigen presentation and CD4+ T cell epitope selection. This unit describes a fairly simple but powerful fluorescence polarization‐based binding competition assay to measure peptide binding to soluble recombinant MHCII molecules. The binding of a peptide of interest to MHCII molecules is assessed based on its ability to inhibit the binding of a fluorescence‐labeled probe peptide, with the strength of binding characterized as IC50 (concentration required for 50% inhibition of probe peptide binding). Data analysis related to this method is discussed. In addition, this unit includes a support protocol for fluorescence labeling peptide using an amine‐reactive probe. The advantage of this protocol is that it allows simple, fast, and high‐throughput measurements of binding for a large set of peptides to MHCII molecules. Curr. Protoc. Immunol. 106:5.10.1‐5.10.12. © 2014 by John Wiley & Sons, Inc.
Myeloid microvesicles in cerebrospinal fluid are associated with myelin damage and neuronal loss in mild cognitive impairment and Alzheimer disease
Authors: Agosta Federica , Dalla Libera Dacia , Spinelli Edoardo Gioele , Finardi Annamaria , Canu Elisa , Bergami Alessandra , Bocchio Chiavetto Luisella , Baronio Manuela , Comi Giancarlo , Martino Gianvito , Matteoli Michela , Magnani Giuseppe , Verderio Claudia , Furlan Roberto 
Published: August 2014

ObjectivesWe have described cerebrospinal fluid (CSF) myeloid microvesicles (MVs) as a marker of microglia activation during neuroinflammation in Alzheimer disease (AD), and characterized their ability to produce toxic amyloid β1–42 (Aβ1–42) oligomers from aggregated or soluble substrate. The aim of this study is to investigate the association of CSF myeloid MVs with neuroimaging, clinical, and paraclinical data in AD and mild cognitive impairment (MCI).MethodsWe collected CSF from 106 AD patients, 51 MCI patients, and 29 neurologically healthy controls. We examined CSF myeloid MV content and AD markers. A subgroup of 34 AD and 21 MCI patients underwent structural and diffusion tensor MRI.ResultsHigher levels of myeloid MVs were found in the CSF of AD patients and MCI patients converting within 3 years relative to controls, but also, at a lower level, in MCI patients not converting to AD. CSF myeloid MVs were associated with Tau but not with Aβ1–42 CSF levels. CSF MVs levels correlated with white matter (WM) tract damage in MCI, and with hippocampal atrophy in AD.InterpretationMicroglial MVs are neurotoxic and myelinotoxic in the presence of Aβ1–42. CSF myeloid MVs, mirroring microglia activation and MV release, are associated with WM damage in MCI and hippocampal atrophy in AD. This suggests that hippocampal microglia activation, in the presence of Aβ1–42 in excess, produces neurotoxic and oligodendrotoxic oligomers that, through WM tract damage, spread disease to neighboring and connected areas, causing local microglia activation and propagation of disease through the same sequence of events. Ann Neurol 2014;76:813–825
High‐Silica Zeolite SSZ‐61 with Dumbbell‐Shaped Extra‐Large‐Pore Channels
Authors: Smeets Stef , Xie Dan , Baerlocher Christian , McCusker Lynne B. , Wan Wei , Zou Xiaodong , Zones Stacey I. 
Published: August 2014

AbstractThe synthesis of the high‐silica zeolite SSZ‐61 using a particularly bulky polycyclic structure‐directing agent and the subsequent elucidation of its unusual framework structure with extra‐large dumbbell‐shaped pore openings are described. By using information derived from a variety of X‐ray powder diffraction and electron microscopy techniques, the complex framework structure, with 20 Si atoms in the asymmetric unit, could be determined and the full structure refined. The Si atoms at the waist of the dumbbell are only three‐connected and are bonded to terminal O atoms pointing into the channel. Unlike the six previously reported extra‐large‐pore zeolites, SSZ‐61 contains no heteroatoms in the framework and can be calcined easily. This, coupled with the possibility of inserting a catalytically active center in the channel between the terminal O atoms in place of H+, afford SSZ‐61 intriguing potential for catalytic applications.
Pineapple agroindustrial residues for the production of high value bacterial cellulose with different morphologies
Authors: Algar Itxaso , Fernandes Susana C. M. , Mondragon Gurutz , Castro Cristina , Garcia‐Astrain Clara , Gabilondo Nagore , Retegi Aloña , Eceiza Arantxa 
Published: August 2014

ABSTRACTBacterial cellulose (BC) with different morphologies was biosynthesized by Gluconacetobacter medellinensis strain under static and dynamic culture conditions using sugar cane juice and pineapple residues as sources of carbon and other nutrients. Hestrin and Schramm's standard culture medium was used as reference. The fermentation condition and resulting yield, physico‐chemical properties, and morphology relationships of obtained cellulose were analyzed. Pineapple agroindustrial residues can be envisaged as an inexpensive and sustainable alternative resource for the production of different BC morphologies. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41237.
Unprecedented Sensitization of Visible and Near‐Infrared Lanthanide Luminescence by Using a Tetrathiafulvalene‐Based Chromophore
Authors: Feng Min , Pointillart Fabrice , Le Guennic Boris , Lefeuvre Bertrand , Golhen Stéphane , Cador Olivier , Maury Olivier , Ouahab Lahcène 
Published: August 2014

AbstractLigand L was synthesized and then coordinated to [Ln(hfac)3]⋅2 H2O (LnIII=Tb, Dy, Er; hfac−=1,1,1,5,5,5‐hexafluoroacetylacetonate anion) and [Ln(tta)3]⋅2 H2O (LnIII=Eu, Gd, Tb, Dy, Er, Yb; tta−=2‐thenoyltrifluoroacetonate) to give two families of dinuclear complexes [Ln2(hfac)6(L)]⋅C6H14 and [Ln2(tta)6(L)]⋅2 CH2Cl2. Irradiation of the ligand at 37 040 cm−1 and 29 410 cm−1 leads to tetrathiafulvalene‐centered and 2,6‐di(pyrazol‐1‐yl)‐4‐pyridine‐centered fluorescence, respectively. The ligand acts as an organic chromophore for the sensitization of the infrared ErIII (6535 cm−1) and YbIII (10 200 cm−1) luminescence. The energies of the singlet and triplet states of L are high enough to guarantee an efficient sensitization of the visible EuIII luminescence (17 300–14 100 cm−1). The EuIII luminescence decay can be nicely fitted by a monoexponential function that allows a lifetime estimation of (0.49±0.01) ms. Finally, the magnetic and luminescence properties of [Yb2(hfac)6(L)]⋅C6H14 were correlated, which allowed the determination of the crystal field splitting of the 2F7/2 multiplet state with MJ=±1/2 as ground states.
Sicherheit von Offshore‐Windenergieanlagen
Authors: Hansen Michael , Schmidt Boso 
Published: August 2014

AbstractDie Offshore‐Windenergie ist mit dem ersten deutschen Offshore‐Windpark alpha ventus in Wassertiefen über 30 m vorgedrungen. Gelten für diese Offshore‐Windenergieanlagen (OWEA), die 60 km vor der Küste stehen, die gleichen Designprinzipien wie für landgestützte Anlagen? Wie sieht es mit dem Risiko dieser Anlagen aus und wie ist dieses definiert?Diese und weitere Fragen führten dazu, dass seit 2009 in dem interfakultativen Verbundforschungsvorhaben Probabilistische Sicherheitsbewertung von Offshore‐Windenergieanlagen (PSB OWEA) zehn Institute aus den Fakultäten Bauingenieurwesen, Elektrotechnik und Maschinenbau der Leibniz Universität Hannover gemeinsam an wesentlichen Fragen zur Anlagensicherheit offshore forschen. Aufgrund des immensen Investitionsvolumens offshore besteht das wesentliche Ziel darin, die Zuverlässigkeit der gesamten Anlage sowie einzelner Anlagenteile und Bauteilkomponenten zu bestimmen und zu bewerten. Die Forschungsschwerpunkte wurden an den Interessen und Notwendigkeiten der Wirtschaft und Zertifizierung gespiegelt und ausgerichtet.In diesem Beitrag werden aktuelle Sicherheitsbetrachtungen für OWEA mit Bezug zu den nationalen und internationalen Regelungen dargestellt.Safety of Offshore Wind TurbinesThe first German offshore wind farm alpha ventus was built in water depth more than 30 m. Are the design principles for these offshore wind turbines (OWT) erected about 60 km distance to the coast similar to the principles used for wind‐turbines onshore? What about the risk of these investments and how is it defined?In 2009 researchers of the Leibniz Universität Hannover started the research project Probabilistic safety assessment of offshore wind turbines (PSA OWT) to think about these and further questions. Since then ten institutes from the faculties of civil engineering, electrical and mechanical engineering research together in the field of the safety of OWT. Regarding the immense volume of offshore investment the target objective is to determine and to evaluate the reliability of the total system as well as individual system components. The main questions were discussed with representatives of industry and certification to bring the interests and needs into line.The current safety assessment of OWT are presented in this paper with respect to national and international regulations.
Automatic analyzer for highly polar carboxylic acids based on fluorescence derivatization–liquid chromatography
Authors: Todoroki Kenichiro , Nakano Tatsuki , Ishii Yasuhiro , Goto Kanoko , Tomita Ryoko , Fujioka Toshihiro , Min Jun Zhe , Inoue Koichi , Toyo'oka Toshimasa 
Published: August 2014

AbstractA sensitive, versatile, and reproducible automatic analyzer for highly polar carboxylic acids based on a fluorescence derivatization–liquid chromatography (LC) method was developed. In this method, carboxylic acids were automatically and fluorescently derivatized with 4‐(N,N‐dimethylaminosulfonyl)‐7‐piperazino‐2,1,3‐benzoxadiazole (DBD‐PZ) in the presence of 4‐(4,6‐dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride by adopting a pretreatment program installed in an LC autosampler. All of the DBD‐PZ‐carboxylic acid derivatives were separated on the ODS column within 30 min by gradient elution. The peak of DBD‐PZ did not interfere with the separation and the quantification of all the acids with the exception of lactic acid. From the LC‐MS/MS analysis, we confirmed that lactic acid was converted to an oxytriazinyl derivative, which was further modified with a dimethoxy triazine group of 4‐(4,6‐dimethoxy‐1,3,5‐triazin‐2‐yl)‐4‐methylmorpholinium chloride (DMT‐MM). We detected this oxytriazinyl derivative to quantify lactic acid. The detection limits (signal‐to‐noise ratio = 3) for the examined acids ranged from 0.19 to 1.1 µm, which correspond to 95–550 fmol per injection. The intra‐ and inter‐day precisions of typical, highly polar carboxylic acids were all <9.0%. The developed method was successfully applied to the comprehensive analysis of carboxylic acids in various samples, which included fruit juices, red wine and media from cultured tumor cells. Copyright © 2014 John Wiley & Sons, Ltd.
Mutations in POLE and survival of colorectal cancer patients – link to disease stage and treatment
Authors: Stenzinger Albrecht , Pfarr Nicole , Endris Volker , Penzel Roland , Jansen Lina , Wolf Thomas , Herpel Esther , Warth Arne , Klauschen Frederick , Kloor Matthias , Roth Wilfried , Bläker Hendrik , Chang‐Claude Jenny , Brenner Hermann , Hoffmeister Michael , Weichert Wilko 
Published: August 2014

AbstractRecent molecular profiling studies reported a new class of ultramutated colorectal cancers (CRCs), which are caused by exonuclease domain mutations (EDMs) in DNA polymerase ϵ (POLE). Data on the clinical implications of these findings as to whether these mutations define a unique CRC entity with distinct clinical outcome are lacking. We performed Sanger sequencing of the POLE exonuclease domain in 431 well‐characterized patients with microsatellite stable (MSS) CRCs of a population‐based patient cohort. Mutation data were analyzed for associations with major epidemiological, clinical, genetic, and pathological parameters including overall survival (OS) and disease‐specific survival (DSS). In 373 of 431 MSS CRC, all exons of the exonuclease domain were analyzable. Fifty‐four mutations were identified in 46 of these samples (12.3%). Besides already reported EDMs, we detected many new mutations in exons 13 and 14 (corresponding to amino acids 410–491) as well as in exon 9 and exon 11 (corresponding to aa 268–303 and aa 341–369). However, we did not see any significant associations of EDMs with clinicopathological parameters, including sex, age, tumor location and tumor stage, CIMP, KRAS, and BRAF mutations. While with a median follow‐up time of 5.0 years, survival analysis of the whole cohort revealed nonsignificantly different adjusted hazard ratios (HRs) of 1.35 (95% CI: 0.82–2.25) and 1.44 (0.81–2.58) for OS and DSS indicating slightly impaired survival of patients with EDMs, subgroup analysis for patients with stage III/IV disease receiving chemotherapy revealed a statistically significantly increased adjusted HR (1.87; 95%CI: 1.02–3.44). In conclusion, POLE EDMs do not appear to define an entirely new clinically distinct disease entity in CRC but may have prognostic or predictive implications in CRC subgroups, whose significance remains to be investigated in future studies.
Assembly of Multiple Components in a Hybrid Microcapsule: Designing a Magnetically Separable Pd Catalyst for Selective Hydrogenation
Authors: Amali Arlin Jose , Sharma Bikash , Rana Rohit Kumar 
Published: August 2014

AbstractIn analogy to the role of long‐chain polyamines in biosilicification, poly‐L‐lysine facilitates the assembly of nanocomponents to design multifunctional microcapsule structures. The method is demonstrated by the fabrication of a magnetically separable catalyst that accommodates Pd nanoparticles (NPs) as active catalyst, Fe3O4 NPs as magnetic component for easy recovery of the catalyst, and silica NPs to impart stability and selectivity to the catalyst. In addition, polyamines embedded inside the microcapsule prevent the agglomeration of Pd NPs and thus result in efficient catalytic activity in hydrogenation reactions, and the hydrophilic silica surface results in selectivity in reactions depending on the polarity of substrates.
Synthesis of Site‐Specifically Phosphate‐Caged siRNAs and Evaluation of Their RNAi Activity and Stability
Authors: Wu Li , Pei Fen , Zhang Jinhao , Wu Junzhou , Feng Mengke , Wang Yuan , Jin Hongwei , Zhang Liangren , Tang Xinjing 
Published: August 2014

AbstractA complete set of new photolabile nucleoside phosphoramidites were synthesized, then site‐specifically incorporated into sense or antisense strands of siRNA for phosphate caging. Single caging modification was made along siRNA strands and their photomodulation of gene silencing were examined by using the firefly luciferase reporter gene. Several key phosphate positions were then identified. Furthermore, multiple caging modifications at these key positions led to significantly enhanced photomodulation of gene silencing activity, suggesting a synergistic effect. The caging group on both the terminally phosphate‐caged siRNA and the single‐stranded caged RNA has comparatively high stability, whereas hydrolysis of the caged group from the internally caged siRNA was observed, irrespective of the presence of Mg2+. Molecular dynamic simulations demonstrated that enhanced hydrolysis of the caging group on internally phosphate‐caged siRNAs was due to easy fragmentation of the caging group upon formation of the pentavalent intermediate of the phosphotriester with attack by water. The caging group in the terminally phosphate‐caged siRNA or single‐stranded caged RNA prefers to form π–π stacks with nearby nucleobases. In addition to providing explanations for previous observations, this study sheds further light on the design of caged oligonucleotides and indicates the direction of future development of nucleic acid drugs with phosphate modifications.
α‐Keto Phenylamides as P1′‐Extended Proteasome Inhibitors
Authors: Voss Constantin , Scholz Christoph , Knorr Sabine , Beck Philipp , Stein Martin L. , Zall Andrea , Kuckelkorn Ulrike , Kloetzel Peter‐Michael , Groll Michael , Hamacher Kay , Schmidt Boris 
Published: August 2014

AbstractThe major challenge for proteasome inhibitor design lies in achieving high selectivity for, and activity against, the target, which requires specific interactions with the active site. Novel ligands aim to overcome off‐target‐related side effects such as peripheral neuropathy, which is frequently observed in cancer patients treated with the FDA‐approved proteasome inhibitors bortezomib (1) or carfilzomib (2). A systematic comparison of electrophilic headgroups recently identified the class of α‐keto amides as promising for next generation drug development. On the basis of crystallographic knowledge, we were able to develop a structure–activity relationship (SAR)‐based approach for rational ligand design using an electronic parameter (Hammett’s σ) and in silico molecular modeling. This resulted in the tripeptidic α‐keto phenylamide BSc4999 [(S)‐3‐(benzyloxycarbonyl‐(S)‐leucyl‐(S)‐leucylamino)‐5‐methyl‐2‐oxo‐N‐(2,4‐dimethylphenyl)hexanamide, 6 a], a highly potent (IC50=38 nM), cell‐permeable, and slowly reversible covalent inhibitor which targets both the primed and non‐primed sites of the proteasome’s substrate binding channel as a special criterion for selectivity. The improved inhibition potency and selectivity of this new α‐keto phenylamide makes it a promising candidate for targeting a wider range of tumor subtypes than commercially available proteasome inhibitors and presents a new candidate for future studies.