bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human Chorionic Membrane‐derived Tunable Hydrogels for Vascular Tissue Engineering Strategies
Authors: Martins Elisa A.G. , Deus Inês A. , Gomes Maria C. , Silva Ana S. , Mano João F. , Custódio Catarina A. 
Published: August 2024

AbstractOne of the foremost targets in the advancement of biomaterials to engineer vascularized tissues is not only to replicate the composition of the intended tissue but also to create thicker structures incorporating a vascular network for adequate nutrients and oxygen supply. For the first time, to the best of current knowledge, a clinically relevant biomaterial is developed, demonstrating that hydrogels made from the human decellularized extracellular matrix can exhibit robust mechanical properties (in the kPa range) and angiogenic capabilities simultaneously. These properties enable the culture and organization of human umbilical vein endothelial cells into tubular structures, maintaining their integrity for 14 days in vitro without the need for additional polymers or angiogenesis‐related factors. This is achieved by repurposing the placenta chorionic membrane (CM), a medical waste with an exceptional biochemical composition, into a valuable resource for bioengineering purposes. After decellularization, the CM underwent chemical modification with methacryloyl groups, giving rise to methacrylated CM (CMMA). CMMA preserved key proteins, as well as glycosaminoglycans. The resulting hydrogels rapidly photopolymerize and have enhanced strength and customizable mechanical properties. Furthermore, they demonstrate angio‐vasculogenic competence in vitro and in vivo, holding significant promise as a humanized platform for the engineering of vascularized tissues.
Light Emissive Nanocomposites Containing a Blue Fluorescent Dicationic Tetraphenylethene and a Red Phosphorescent [Mo6I8(OCOC2F5)6]2− Octahedral Cluster for Optical Writing
Authors: Kashnik Ilya V. , Yang Binying , Dumait Noée , Cordier Marie , Brylev Konstantin A. , Bouit Pierre‐Antoine , Molard Yann 
Published: August 2024

AbstractAn emissive organic–inorganic hybrid material is obtained by a cationic metathesis between a blue fluorescent tetraphenylethylene‐based salt: [TPEIm2]I2, and a red‐NIR phosphorescent octahedral molybdenum cluster compound: Cs2[Mo6I8(OCOC2F5)6]. As demonstrated by Single Crystal X‐Ray Diffraction, the hybrid [TPEIm2][Mo6I8(OCOC2F5)6] crystallizes as a 2:2 dimer and emits in the red‐NIR area. Its emission properties are studied in the solid state and once embedded in poly(methylmethacrylate) by steady state and time dependent photoluminescence spectroscopy. They are further compared to the case in which the two starting salts are directly integrated in the polymer matrix. Obtained nanocomposites show evanescent UV‐2A optical writing and reading behavior, with color contrasts depending on how emitters have been introduced.
Extracellular Vesicles from Compression‐Loaded Cementoblasts Promote the Tissue Repair Function of Macrophages
Authors: Yang Yuhui , Liu Hao , Guo Kunyao , Yu Qianyao , Zhao Yi , Wang Jiayi , Huang Yiping , Li Weiran 
Published: August 2024

AbstractTreatment strategies for hard tissue defects aim to establish a mineralized microenvironment that facilitates tissue remodeling. As a mineralized tissue, cementum shares a similar structure with bone and exhibits an excellent capacity to resist resorption under compression. Macrophages are crucial for mineralized remodeling; however, their functional alterations in the microenvironment of cementum remain poorly understood. Therefore, this study explores the mechanisms by which cementum resists resorption under compression and the regulatory roles of cementoblasts in macrophage functions. As a result, extracellular vesicles from compression‐loaded cementoblasts (Comp‐EVs) promote macrophage M2 polarization and enhance the clearance of apoptotic cells (efferocytosis) by 2‐ to 3‐fold. Local injection of Comp‐EVs relieves cementum destruction in mouse root resorption model by activating the tissue repair function of macrophages. Moreover, Comp‐EV‐loaded hydrogels achieve significant bone healing in calvarial bone defect. Unexpectedly, under compression, EV secretion in cementoblasts is reduced by half. RNA‐Seq analysis and verification reveal that Rab35 expression decreases by 60% under compression, thereby hampering the release of EVs. Rab35 overexpression is proposed as a modification of cementoblasts to boost the yield of Comp‐EVs. Collectively, Comp‐EVs activate the repair function of macrophages, which will be a potential therapeutic strategy for hard tissue repair and regeneration.
Ultrafast Two‐Color X‐Ray Emission Spectroscopy Reveals Excited State Landscape in a Base Metal Dyad
Authors: Nowakowski Michal , Huber‐Gedert Marina , Elgabarty Hossam , Kalinko Aleksandr , Kubicki Jacek , Kertmen Ahmet , Lindner Natalia , Khakhulin Dmitry , Lima Frederico A. , Choi Tae‐Kyu , Biednov Mykola , Schmitz Lennart , Piergies Natalia , Zalden Peter , Kubicek Katharina , Rodriguez‐Fernandez Angel , Salem Mohammad Alaraby , Canton Sophie E. , Bressler Christian , Kühne Thomas D. , Gawelda Wojciech , Bauer Matthias 
Published: August 2024

AbstractEffective photoinduced charge transfer makes molecular bimetallic assemblies attractive for applications as active light‐induced proton reduction systems. Developing competitive base metal dyads is mandatory for a more sustainable future. However, the electron transfer mechanisms from the photosensitizer to the proton reduction catalyst in base metal dyads remain so far unexplored. A Fe─Co dyad that exhibits photocatalytic H2 production activity is studied using femtosecond X‐ray emission spectroscopy, complemented by ultrafast optical spectroscopy and theoretical time‐dependent DFT calculations, to understand the electronic and structural dynamics after photoexcitation and during the subsequent charge transfer process from the FeII photosensitizer to the cobaloxime catalyst. This novel approach enables the simultaneous measurement of the transient X‐ray emission at the iron and cobalt K‐edges in a two‐color experiment. With this methodology, the excited state dynamics are correlated to the electron transfer processes, and evidence of the Fe→Co electron transfer as an initial step of proton reduction activity is unraveled.
Generation of Anti‐Mastitis Gene‐Edited Dairy Goats with Enhancing Lysozyme Expression by Inflammatory Regulatory Sequence using ISDra2‐TnpB System
Authors: Feng Rui , Zhao Jianglin , Zhang Qian , Zhu Zhenliang , Zhang Junyu , Liu Chengyuan , Zheng Xiaoman , Wang Fan , Su Jie , Ma Xianghai , Mi Xiaoyu , Guo Lin , Yan Xiaoxue , Liu Yayi , Li Huijia , Chen Xu , Deng Yi , Wang Guoyan , Zhang Yong , Liu Xu , Liu Jun 
Published: August 2024

AbstractGene‐editing technology has become a transformative tool for the precise manipulation of biological genomes and holds great significance in the field of animal disease‐resistant breeding. Mastitis, a prevalent disease in animal husbandry, imposes a substantial economic burden on the global dairy industry. In this study, a regulatory sequence gene editing breeding strategy for the successful creation of a gene‐edited dairy (GED) goats with enhanced mastitis resistance using the ISDra2‐TnpB system and dairy goats as the model animal is proposed. This included the targeted integration of an innate inflammatory regulatory sequence (IRS) into the promoter region of the lysozyme (LYZ) gene. Upon Escherichia Coli (E. coli) mammary gland infection, GED goats exhibited increased LYZ expression, showing robust anti‐mastitis capabilities, mitigating PANoptosis activation, and alleviating blood‐milk‐barrier (BMB) damage. Notably, LYZ is highly expressed only in E. coli infection. This study marks the advent of anti‐mastitis gene‐edited animals with exogenous‐free gene expression and demonstrates the feasibility of the gene‐editing strategy proposed in this study. In addition, it provides a novel gene‐editing blueprint for developing disease‐resistant strains, focusing on disease specificity and biosafety while providing a research basis for the widespread application of the ISDra2‐TnpB system.
2D Super‐Resolution Metrology Based on Superoscillatory Light
Authors: Wang Yu , Chan Eng Aik , Rendón‐Barraza Carolina , Shen Yijie , Plum Eric , Ou Jun‐Yu 
Published: August 2024

AbstractProgress in the semiconductor industry relies on the development of increasingly compact devices consisting of complex geometries made from diverse materials. Precise, label‐free, and real‐time metrology is needed for the characterization and quality control of such structures in both scientific research and industry. However, optical metrology of 2D sub‐wavelength structures with nanometer resolution remains a major challenge. Here, a single‐shot and label‐free optical metrology approach that determines 2D features of nanostructures, is introduced. Accurate experimental measurements with a random statistical error of 18 nm (λ/27) are demonstrated, while simulations suggest that 6 nm (λ/81) may be possible. This is far beyond the diffraction limit that affects conventional metrology. This metrology employs neural network processing of images of the 2D nano‐objects interacting with a phase singularity of the incident topologically structured superoscillatory light. A comparison between conventional and topologically structured illuminations shows that the presence of a singularity with a giant phase gradient substantially improves the retrieval of object information in such an optical metrology. This non‐invasive nano‐metrology opens a range of application opportunities for smart manufacturing processes, quality control, and advanced materials characterization.
Lactylome Analysis Unveils Lactylation‐Dependent Mechanisms of Stemness Remodeling in the Liver Cancer Stem Cells
Authors: Feng Fan , Wu Jiaqin , Chi Qingjia , Wang Shunshun , Liu Wanqian , Yang Li , Song Guanbin , Pan Lianhong , Xu Kang , Wang Chunli 
Published: August 2024

AbstractLactate plays a critical role as an energy substrate, metabolite, and signaling molecule in hepatocellular carcinoma (HCC). Intracellular lactate‐derived protein lysine lactylation (Kla) is identified as a contributor to the progression of HCC. Liver cancer stem cells (LCSCs) are believed to be the root cause of phenotypic and functional heterogeneity in HCC. However, the impact of Kla on the biological processes of LCSCs remains poorly understood. Here enhanced glycolytic metabolism, lactate accumulation, and elevated levels of lactylation are observed in LCSCs compared to HCC cells. H3K56la was found to be closely associated with tumourigenesis and stemness of LCSCs. Notably, a comprehensive examination of the lactylome and proteome of LCSCs and HCC cells identified the ALDOA K230/322 lactylation, which plays a critical role in promoting the stemness of LCSCs. Furthermore, this study demonstrated the tight binding between aldolase A (ALDOA) and dead box deconjugate enzyme 17 (DDX17), which is attenuated by ALDOA lactylation, ultimately enhancing the regulatory function of DDX17 in maintaining the stemness of LCSCs. This investigation highlights the significance of Kla in modulating the stemness of LCSCs and its impact on the progression of HCC. Targeting lactylation in LCSCs may offer a promising therapeutic approach for treating HCC.
Poly(benzodifurandione) Coated Silk Yarn for Thermoelectric Textiles
Authors: Craighero Mariavittoria , Li Qifan , Zeng Zijin , Choi Chunghyeon , Kim Youngseok , Yoon Hyungsub , Liu Tiefeng , Sowinski Przemyslaw , Haraguchi Shuichi , Hwang Byungil , Mihiretie Besira , Fabiano Simone , Müller Christian 
Published: August 2024

AbstractThermoelectric textile devices represent an intriguing avenue for powering wearable electronics. The lack of air‐stable n‐type polymers has, until now, prevented the development of n‐type multifilament yarns, which are needed for textile manufacturing. Here, the thermomechanical properties of the recently reported n‐type polymer poly(benzodifurandione) (PBFDO) are explored and its suitability as a yarn coating material is assessed. The outstanding robustness of the polymer facilitates the coating of silk yarn that, as a result, displays an effective bulk conductivity of 13 S cm−1, with a projected half‐life of 3.2 ± 0.7 years at ambient conditions. Moreover, the n‐type PBFDO coated silk yarn with a Young's modulus of E = 0.6 GPa and a strain at break of εbreak = 14% can be machine washed, with only a threefold decrease in conductivity after seven washing cycles. PBFDO and poly(3,4‐ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) coated silk yarns are used to fabricate two out‐of‐plane thermoelectric textile devices: a thermoelectric button and a larger thermopile with 16 legs. Excellent air stability is paired with an open‐circuit voltage of 17 mV and a maximum output power of 0.67 µW for a temperature difference of 70 K. Evidently, PBFDO coated multifilament silk yarn is a promising component for the realization of air stable thermoelectric textile devices.
Optimizing the Ferroelectric Performance of Hf0.5Zr0.5O2 Epitaxial Film by La0.67Sr0.33MnO3 Capping Layer
Authors: Liu Kuan , Liu Kai , Zhang Xingchang , Jin Feng , Fang Jie , Hua Enda , Ye Huan , Zhang Jinfeng , Liang Zhengguo , Lv Qiming , Wu Wenbin , Ma Chao , Wang Lingfei 
Published: August 2024

AbstractHafnium‐oxide‐based ferroelectrics have garnered considerable research interest, primarily for their robust ferroelectricity at the nanoscale and their high compatibility with complementary metal‐oxide‐semiconductors processes. However, the impact of electrodes on the ferroelectric properties of hafnium‐oxide layer, particularly that of top electrodes, is not yet fully understood even in the simplest capacitor geometry. In this study, the La0.67Sr0.33MnO3/Hf0.5Zr0.5O2 (LSMO/HZO) epitaxial heterostructure is utilized as a model system to conduct a systematic comparative study on ferroelectricity between the LSMO/HZO (H‐LS) bilayer and LSMO/HZO/LSMO (LS‐H‐LS) trilayer samples. In comparison to the H‐LS sample, the LS‐H‐LS sample exhibits a more uniform polar domain configuration and larger ferroelectric polarization. Moreover, the LS‐H‐LS sample exhibits significant improvements in leakage, endurance, and retention. These substantial enhancements in ferroelectricity are likely due to interfacial stress imposed by the LSMO capping layer and its capacity to accommodate extra oxygen vacancies. These results underscore the pivotal role of oxide‐based top electrodes in determining the ferroelectricity of hafnium‐oxide‐based heterostructures, providing crucial insights for optimizing the performance of innovative ferroelectric devices.
The response of a sorghum sudangrass cover crop to residual nitrogen and its relationship with spectral sensors
Authors: Netto‐Ferreira Julia Barra , de Sá Leitão Diego Arruda Huggins , Griffin Winniefred , Sharma Lakesh K. , Maltais‐Landry Gabriel 
Published: August 2024

AbstractA sorghum sudangrass (SSG) cover crop grown after a cash crop could take up residual nitrogen (N) before it is lost. As in‐field monitoring of SSG properties is laborious, predicting biomass and N concentrations with spectral sensors could be useful. At two sites in Live Oak, Florida, we evaluated the response of SSG to residual N from previous N fertilization and the performance of handheld and satellite sensors in estimating SSG properties. We quantified aboveground biomass, plant N, leaf greenness (NDVI), net potential N mineralization (PNM), and soil permanganate oxidizable carbon (POXC). Residual N did not affect SSG properties, PNM was highest at the highest N input rate in one site, and soil POXC was correlated with SSG properties (biomass and plant N). NDVI measured from a handheld sensor better predicted SSG properties than satellite imagery in these small plots, suggesting a greater potential to be a useful management tool.
Development and assessment of analytic methods to improve the measurement of cognition in longitudinal studies of aging through the use of substudies with comprehensive neuropsychological testing
Authors: Nichols Emma , Jones Richard N. , Gross Alden L. , Hayat Shabina , Zaninotto Paola , Lee Jinkook 
Published: August 2024

AbstractINTRODUCTIONThe Health and Retirement Study International Partner Surveys (HRS IPS) have rich longitudinal data, but the brevity of cognitive batteries is a limitation.METHODSWe used data from a substudy of the English Longitudinal Study of Ageing (ELSA) administering detailed cognitive assessments with the Harmonized Cognitive Assessment Protocol (ELSA‐HCAP) (N = 1273) to inform approaches for estimating cognition in ELSA (N = 11,213). We compared two novel approaches: confirmatory factor analysis (CFA)‐ and regression‐based prediction.RESULTSCompared to estimates from the full HCAP battery, estimated cognitive functioning derived using regression models or CFA had high correlations (regression: r = 0.85 [95% confidence interval [CI]: 0.83 to 0.87]; CFA: r = 0.83 [95% CI: 0.81 to 0.85]) and reasonable mean squared error (regression: 0.25 [0.22 to 0.27]; CFA: 0.29 [0.26 to 0.32]) in held‐out data. The use of additional items from waves 7 to 9 improved performance.DISCUSSIONBoth approaches are recommended for future research; the similarity in approaches may be due to the brevity of available cognitive assessments in ELSA.Highlights
Estimates of cognitive functioning informed by English Longitudinal Study of Ageing‐Harmonized Cognitive Assessment Protocol (ELSA‐HCAP) data had an adequate performance.
Standard errors were smaller for associations with example risks when using measures informed by ELSA‐HCAP.
Performance was better when including additional cognitive measures available in waves 7 to 9.
Conceptual advantages to the confirmatory factor analysis (CFA) approach were not important in practice due to the brevity of the ELSA cognitive battery.
Peptide ligands for the universal purification of exosomes by affinity chromatography
Authors: Kilgore Ryan E. , Moore Brandyn D. , Sripada Sobhana A. , Chu Wenning , Shastry Shriarjun , Barbieri Eduardo , Hu Shiqi , Tian Weihua , Petersen Heidi , Mohammadifar Mohammad , Simpson Aryssa , Brown Ashley , Lavoie Joseph , Elhanafi Driss , Goletz Steffen , Cheng Ke , Daniele Michael A. , Menegatti Stefano 
Published: August 2024

AbstractExosomes are gaining prominence as vectors for drug delivery, vaccination, and regenerative medicine. Owing to their surface biochemistry, which reflects the parent cell membrane, these nanoscale biologics feature low immunogenicity, tunable tissue tropism, and the ability to carry a variety of payloads across biological barriers. The heterogeneity of exosomes' size and composition, however, makes their purification challenging. Traditional techniques, like ultracentrifugation and filtration, afford low product yield and purity, and jeopardizes particle integrity. Affinity chromatography represents an excellent avenue for exosome purification. Yet, current affinity media rely on antibody ligands whose selectivity grants high product purity, but mandates the customization of adsorbents for exosomes with different surface biochemistry while their binding strength imposes elution conditions that may harm product's activity. Addressing these issues, this study introduces the first peptide affinity ligands for the universal purification of exosomes from recombinant feedstocks. The peptides were designed to (1) possess promiscuous biorecognition of exosome markers, without binding process‐related contaminants and (2) elute the product under conditions that safeguard product stability. Selected ligands SNGFKKHI and TAHFKKKH demonstrated the ability to capture of exosomes secreted by 14 cell sources and purified exosomes derived from HEK293, PC3, MM1, U87, and COLO1 cells with yields of up to 80% and up‐to 50‐fold reduction of host cell proteins (HCPs) upon eluting with pH gradient from 7.4 to 10.5, recommended for exosome stability. SNGFKKHI‐Toyopearl resin was finally employed in a two‐step purification process to isolate exosomes from HEK293 cell fluids, affording a yield of 68% and reducing the titer of HCPs to 68 ng/mL. The biomolecular and morphological features of the isolated exosomes were confirmed by analytical chromatography, Western blot analysis, transmission electron microscopy, nanoparticle tracking analysis.
Characterizing white matter connectome abnormalities in patients with temporal lobe epilepsy using threshold‐free network‐based statistics
Authors: Chu Daniel Y , Imhoff‐Smith Theodore P , Nair Veena A , Choi Timothy , Adluru Anusha , Garcia‐Ramos Camille , Dabbs Kevin , Mathis Jedidiah , Nencka Andrew S , Conant Lisa , Binder Jeffrey R , Meyerand Mary E , Alexander Andrew L , Struck Aaron F , Hermann Bruce , Prabhakaran Vivek , Adluru Nagesh 
Published: August 2024

AbstractIntroductionEmerging evidence illustrates that temporal lobe epilepsy (TLE) involves network disruptions represented by hyperexcitability and other seizure‐related neural plasticity. However, these associations are not well‐characterized. Our study characterizes the whole brain white matter connectome abnormalities in TLE patients compared to healthy controls (HCs) from the prospective Epilepsy Connectome Project study. Furthermore, we assessed whether aberrant white matter connections are differentially related to cognitive impairment and a history of focal‐to‐bilateral tonic–clonic (FBTC) seizures.MethodsMulti‐shell connectome MRI data were preprocessed using the DESIGNER guidelines. The IIT Destrieux gray matter atlas was used to derive the 162 × 162 structural connectivity matrices (SCMs) using MRTrix3. ComBat data harmonization was applied to harmonize the SCMs from pre‐ and post‐scanner upgrade acquisitions. Threshold‐free network‐based statistics were used for statistical analysis of the harmonized SCMs. Cognitive impairment status and FBTC seizure status were then correlated with these findings.ResultsWe employed connectome measurements from 142 subjects, including 92 patients with TLE (36 males, mean age = 40.1 ± 11.7 years) and 50 HCs (25 males, mean age = 32.6 ± 10.2 years). Our analysis revealed overall significant decreases in cross‐sectional area (CSA) of the white matter tract in TLE group compared to controls, indicating decreased white matter tract integrity and connectivity abnormalities in addition to apparent differences in graph theoretic measures of connectivity and network‐based statistics. Focal and generalized cognitive impaired TLE patients showcased higher trend‐level abnormalities in the white matter connectome via decreased CSA than those with no cognitive impairment. Patients with a positive FBTC seizure history also showed trend‐level findings of association via decreased CSA.ConclusionsWidespread global aberrant white matter connectome changes were observed in TLE patients and characterized by seizure history and cognitive impairment, laying a foundation for future studies to expand on and validate the novel biomarkers and further elucidate TLE's impact on brain plasticity.