bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adiponectin receptors agonist alters microbiota to improve implant osseointegration in diabetic mice
Authors: Wang Xia , Lin Linni , Yao Qianqian 
Published: August 2024

AbstractObjectiveEstimate the impact of Adiponectin receptors agonist (AdipoRon) on dental implant osseointegration in alveolar bone and explore the possible mechanism between saliva microbiota and AdipoRon in diabetic mice.Materials and MethodsSixty C57BL/6 mice (male, 8 weeks old) were divided randomly into four groups according to different doses of AdipoRon: normoglycemic control group; DM control group; DM with a low dose of AdipoRon (5 mg/kg/day); and DM with a high dose of AdipoRon (50 mg/kg/day). Then, dental implants were placed in the palatal root socket in the first molar extraction mouse model. Micro‐computed tomography, histology examination, immunohistochemical staining, and oral microbiota were explored to evaluate implant osseointegration.ResultsAdipoRon treatment at 50 mg/kg markedly promoted dental implant osseointegration in diabetic mice, but AdipoRon treatment at 5 mg/kg was not effective. Moreover, distinct differences in the oral microbiota composition were shown between the diabetic mice and diabetic mice treated with AdipoRon at 50 mg/kg.ConclusionAdipoRon treatment at 50 mg/kg in diabetic mice could significantly increase dental implant osseointegration. The salivary microbiota might participate in the accelerated osseointegration progress of dental implants in AdipoRon treatment.
Multiplex Expression Cassette Assembly: A flexible and versatile method for building complex genetic circuits in conventional vectors
Authors: Jiang Xun , Zhang Zhuoxiang , Wu Xiuming , Li Changmei , Sun Xuan , Li Yiting , Chang Aixia , Yang Aiguo , Yang Changqing 
Published: August 2024

SummaryThe manipulation of multiple transcription units for simultaneous and coordinated expression is not only key to building complex genetic circuits to accomplish diverse functions in synthetic biology, but is also important in crop breeding for significantly improved productivity and overall performance. However, building constructs with multiple independent transcription units for fine‐tuned and coordinated regulation is complicated and time‐consuming. Here, we introduce the Multiplex Expression Cassette Assembly (MECA) method, which modifies canonical vectors compatible with Golden Gate Assembly, and then uses them to produce multi‐cassette constructs. By embedding the junction syntax in primers that are used to amplify functional elements, MECA is able to make complex constructs using only one intermediate vector and one destination vector via two rounds of one‐pot Golden Gate assembly reactions, without the need for dedicated vectors and a coherent library of standardized modules. As a proof‐of‐concept, we modified eukaryotic and prokaryotic expression vectors to generate constructs for transient expression of green fluorescent protein and β‐glucuronidase in Nicotiana benthamiana, genome editing to block monoterpene metabolism in tomato glandular trichomes, production of betanin in tobacco and synthesis of β‐carotene in Escherichia coli. Additionally, we engineered the stable production of thymol and carvacrol, bioactive compounds from Lamiaceae family plants, in glandular trichomes of tobacco. These results demonstrate that MECA is a flexible, efficient and versatile method for building complex genetic circuits, which will not only play a critical role in plant synthetic biology, but also facilitate improving agronomic traits and pyramiding traits for the development of next‐generation elite crops.
Introduction of broadleaf tree species can promote the resource use efficiency and gross primary productivity of pure forests
Authors: Liu Ziqiang , Wang Xiaodi , Jia Guodong , Jiang Jiang , Liao Bin 
Published: August 2024

AbstractLong‐term pure forest (PF) management and successive planting has result resulted in “low‐efficiency artificial forests” in large areas. However, controversy persists over the promoting effect of introduction of broadleaf tree species on production efficiency of PF. This study hypothesised that introduced broadleaf tree species can significantly promote both water‐nutrient use efficiency and gross primary productivity (GPP)of PF. Tree ring chronologies, water source, water use efficiency and GPP were analysed in coniferous Cunninghamia lanceolata and broadleaved Phoebe zhennan growing over the past three decades. The introduction of P. zhennan into C. lanceolata plantations resulted in inter‐specific competition for water, probably because of the similarity of the main water source of these two tree species. However, C. lanceolata absorbed more water with a higher nutrient level from the 40–60‐cm soil layer in mixed forests (MF). Although the co‐existing tree species limited the basal area increment and growth rates of C. lanceolata in MF plots, the acquisition of dissolved nutrients from the fertile topsoil layer were enhanced; this increased the water use efficiency and GPP of MF plots. To achieve better ecological benefits and GPP, MFs should be constructed in southern China.
Hydrogen sulphide alleviates root growth inhibition induced by phosphate starvation
Authors: Liu Tong , Chen Hao , Luo Sheng , Xue Shaowu 
Published: August 2024

AbstractPhosphorus (P) has crucial roles in plant growth and development. Hydrogen sulphide (H2S) has multiple functions in plants, particularly having the ability to promote tolerance to a variety of adversity stresses. However, it is unclear whether H2S has a function when plants suffer Pi‐deficiency stress. DES1, encoding L‐cysteine desulfhydrase1, is a crucial source of H2S in Arabidopsis thaliana by catalysing the substrate L‐cysteine. Under phosphate starvation, the des1 mutant had a significantly shorter primary root length than the wild‐type Col‐0, and exogenous application of H2S donor NaHS could compensate for the root growth‐sensitive phenotype. In contrast, the transgenic lines DES1ox overexpressing DES1 exhibited less sensitivity to phosphate starvation in terms of longer roots compared to the Col‐0. These results demonstrate that H2S is involved in the regulation of Arabidopsis root growth under phosphate starvation. Moreover, using quantitative real‐time polymerase chain reaction experiments to analyse the changes in genes induced by phosphate starvation in des1 mutant and Col‐0, we screened to find that the expression of the Sulfoquinovosyl diacylglycerol 1 (SQD1) gene was significantly downregulated in the des1 mutant. Consistently, exogenous H2S significantly promoted SQD1 expression levels in roots of Col‐0. Taken together, we demonstrate that DES1‐mediated H2S participates in alleviating root growth inhibition by promoting the expression of SQD1 under Pi starvation.
A new end‐user–oriented and dynamic approach to post‐disaster resilience quantification for individual facilities
Authors: Cremen Gemma 
Published: August 2024

AbstractCommunity recovery from a disaster is a complex process, in which the importance of different types of infrastructure functionality can change over time. Most of the myriad of metrics available for measuring disaster resilience do not capture the dynamic importance of functionality explicitly, however. This means that very different recovery trajectories of a given infrastructure can correspond to the same resilience value, regardless of variations in its utility over time. While some efforts have been made to integrate features of time dependency into individual facility resilience quantification, the resulting metrics either capture only a limited set of temporal instances throughout the post‐disaster phase or do not offer a way to prioritize time steps in line with variations in the importance of facility functionality. This study proposes a novel, straightforward metric for component‐level post‐disaster resilience quantification that overcomes the aforementioned limitations. The metric involves a dynamic weighting component that enables stakeholders to place varying emphasis on different temporal points throughout the recovery process. The end‐user–centered approach to resilience quantification facilitated by the metric allows for flexible, context‐specific interpretations of infrastructure functionality importance that may vary across different communities. The metric is demonstrated through a hypothetical case study of infrastructure facilities with varying degrees of importance across the post‐disaster recovery period, which showcases its versatility relative to a previously well‐established measurement of component‐level resilience. The proposed metric has significant potential for use in stakeholder‐driven approaches to decision making on critical infrastructure (as well as other types of built environment) recovery and resilience.
The impact of trade barriers on outward foreign direct investment: Evidence from Chinese manufacturing firms
Authors: Yuan Chunlai , Han Zhaojie , Wu Haitao 
Published: August 2024

AbstractIn recent years, escalating trade frictions and the surge in “anti‐globalization” sentiments have caught global attention. It is well‐understood that excessive trade barriers can hinder international trade and economic interactions. Our research, utilizing a mixed logit model, discerns the impact of these barriers on outward foreign direct investment (OFDI). Findings reveal trade barriers notably enhance OFDI activities among Chinese manufacturing firms, especially those receiving subsidies or trading with developing nations. Key mechanisms include the export substitution effect, investment market diversification, and profit‐driven effects. The study also underscores that tariff barriers more significantly affect capital‐intensive and foreign‐funded firms, whereas non‐tariff barriers influence technology‐intensive enterprises more. This research offers fresh insights into OFDI in manufacturing and evaluates the economic repercussions of trade barriers.
Hamiltonian mechanics of “magnetic” solitons in two‐component Bose–Einstein condensates
Authors: Kamchatnov A. M. 
Published: August 2024

AbstractWe consider the motion of a “magnetic” soliton in two‐component condensates along a nonuniform and time‐dependent background in the framework of Hamiltonian mechanics. Our approach is based on generalization of Stokes' remark that soliton's velocity is related to its inverse half‐width by the dispersion law for linear waves continued to the region of complex wave numbers. We obtain expressions for the canonical momentum and the Hamiltonian as functions of soliton's velocity and transform the Hamilton equations to a Newton‐like equation. The theory is illustrated by several examples of concrete soliton's dynamics.
A dominant‐negative Arabidopsis cation exchanger 1 (CAX1): N‐terminal autoinhibition and membrane topology
Authors: Sarkar Shayan , Rhein Hormat Shadgou , Pittman Jon K. , Hirschi Kendal D. 
Published: August 2024

SUMMARYCalcium (Ca2+) is essential for plant growth and cellular homeostasis, with cation exchangers (CAXs) regulating Ca2+ transport into plant vacuoles. In Arabidopsis, multiple CAXs feature a common structural arrangement, comprising an N‐terminal autoinhibitory domain followed by two pseudosymmetrical modules. Mutations in CAX1 enhance stress tolerance, notably tolerance to anoxia (a condition marked by oxygen depletion), crucial for flood resilience. Here we engineered a dominant‐negative CAX1 variant, named ½N‐CAX1, incorporating the autoinhibitory domain and the N‐terminal pseudosymmetrical module, which, when expressed in wild‐type Arabidopsis plants, phenocopied the anoxia tolerance of cax1. Physiological evaluations, yeast assays, and calcium imaging demonstrated that wild‐type plants expressing ½N‐CAX1 have phenotypes consistent with inhibition of CAX1, which is likely through direct interaction of ½N‐CAX1 with CAX1. Eliminating segments within the N‐terminal pseudosymmetrical module, as well as incorporating modules from other plant CAXs and expressing these variants into wild‐type plants, failed to produce anoxia tolerance. This underscores the requirement for both the CAX1 autoinhibitory domain and the intact pseudosymmetrical module to produce the dominant‐negative phenotype. Our study elucidates the interaction of this ½N‐CAX1 variant with CAX1 and its impact on anoxia tolerance, offering insights into further approaches for engineering plant stress tolerance.
Evaluation of 0.1% and 1% atropine eyedrops in cats: A comparative study of tolerance, stability, and efficacy
Authors: Handel Karin W. , Ofri Ron , Goncharov Yulia , Arad Dikla , Sebbag Lionel 
Published: August 2024

AbstractObjectiveInvestigate the tolerance, stability, and efficacy of topical 0.1% and 1% atropine in cats.ProceduresSix cats underwent two trials separated by a 2‐week washout period. One drop of artificial tears was placed in one randomly selected eye (control), and one drop of either 0.1% atropine (Trial I) or 1% atropine (Trial II) was placed in the other eye. Immediate adverse effects were recorded for severity (0–3) and duration (seconds). Horizontal pupil diameter (HPD), pupillary light reflexes (PLRs), intraocular pressure (IOP), Schirmer tear test‐1 (STT‐1), and heart rate (HR) were monitored at baseline then 8 h post‐administration. PLRs were assessed for a total of 72 h. Stability was assessed weekly for 1 month in room temperature and refrigerated conditions, evaluating solution clarity, pH, and drug concentrations.ResultsAdverse effects had a significantly lower severity score and shorter duration with 0.1% versus 1% atropine (severity 1.2 ± 0.4 vs. 2.5 ± 0.5, p = .010; duration 107.5 ± 53.3 vs. 293.3 ± 106.5 s, p = .009). HPD was significantly greater than baseline measurements as early as 40 min for both atropine formulations. Pupils were non‐responsive for a significantly shorter duration with 0.1% versus 1% atropine (median 7 h vs. 47.5 h, p = .031). Compared with control eyes, IOP was significantly elevated by 1% atropine (p = .021) but not 0.1% atropine (p = .502). No significant differences were noted in STT‐1 and HR measurements. Both solutions were stable in room temperature and refrigerated conditions for 1 month.ConclusionsDiluted 0.1% atropine was stable and better tolerated by cats, offering a potential alternative to feline patients that experience adverse effects from topical 1% atropine.
Global classical solutions to a multidimensional radiation hydrodynamics model with symmetry and large initial data
Authors: Wei Jing , Zhang Minyi , Zhu Changjiang 
Published: August 2024

AbstractAs a first stage to study the global large solutions of the radiation hydrodynamics model with viscosity and thermal conductivity in the high‐dimensional space, we study the problems in high dimensions with some symmetry, such as the spherically or cylindrically symmetric solutions. Specifically, we will study the global classical large solutions to the radiation hydrodynamics model with spherically or cylindrically symmetric initial data. The key point is to obtain the strict positive lower and upper bounds of the density and the lower bound of the temperature . Compared with the Navier–Stokes equations, these estimates in the present paper are more complicated due to the influence of the radiation. To overcome the difficulties caused by the radiation, we construct a pointwise estimate between the radiative heat flux and the temperature by studying the boundary value problem of the corresponding ordinary differential equation. And we consider a general heat conductivity: if ; if . This can be viewed as the first result about the global classical large solutions of the radiation hydrodynamics model with some symmetry in the high‐dimensional space.
Is there reversible dimerization of albumin in blood plasma? And does it matter?
Authors: Harris Gemma , Bradshaw Michelle L. , Halsall David J. , Scott David J. , Unwin Robert J. , Norden Anthony G. W. 
Published: August 2024

AbstractMost albumin in blood plasma is thought to be monomeric with some 5% covalently dimerized. However, many reports in the recent biophysics literature find that albumin is reversibly dimerized or even oligomerized. We review data on this from X‐ray crystallography and diverse biophysical techniques. The number‐average molecular weight of albumin would be increased by dimerization, affecting size‐dependent filtration processes of albumin such as at the glycocalyx of the capillary endothelium and the podocyte slit‐diaphragm of the renal glomerulus. If correct, and depending on characteristics of the process, such as Kd, reversible dimerization of albumin in plasma would have major implications for normal physiology and medicine. We present quantitative models of the impact of dimerization on albumin molecular forms, on the number‐average molecular weight of albumin, and estimate the effect on the colloid osmotic pressure of albumin. Dimerization reduces colloid osmotic pressure as total albumin concentration increases below that expected in the absence of dimerization. Current models of albumin filtration by the renal glomerulus would need revision to account for the dynamic size of albumin molecules filtered. More robust biophysical data are needed to give a definitive answer to the questions posed and we suggest possible approaches to this.
High-Order Theory Approach for Debonded Sandwich Panels—Part II: Energy Release Rate and Mode Mixity
Authors: Yuan Zhangxian , Kardomateas George A. 
Published: August 2024

Abstract
A novel re-formulation of the extended high-order sandwich panel theory (EHSAPT) for the case of a face/core debond was done in Part I of this two-part series of papers. This high-order theory is capable of including not only the transverse shear but also the transverse compressibility and axial rigidity of the core. In this Part II paper, a closed-form expression for the energy release rate is derived in terms of the equivalent resultant forces and displacements at the two edge sections. Together with the crack surface displacement method, the mode mixity is determined from the displacement field. Benefiting from the accurate displacement field given by the proposed theory, this paper presents a self-contained approach for the mode mixity that is free of parameters that need to be determined via additional numerical simulations (i.e., no extrapolation needed). Results are compared with the ones given by the classical theory in the literature and the ones given by the finite element method with a very fine mesh. The accuracy is proven for a wide range of core materials from the stiffer cores to the very compliant ones, and for a wide range of debond lengths.
High-Order Theory Approach for Debonded Sandwich Panels—Part I: Formulation and Displacement Fields
Authors: Yuan Zhangxian , Kardomateas George A. 
Published: August 2024

Abstract
A high-order theory is developed to model asymmetric sandwich panels with face/core debonds and to provide a solution for the energy release rate and mode mixty. This new theory is a novel re-formulation of the extended high-order sandwich panel theory (EHSAPT), in which faces and core were originally considered to be perfectly bonded. In the new formulation, a sandwich panel with an interfacial debond can be divided into three parts, namely, the debonded part, the substrate part, and the base part. A new high-order displacement pattern is developed to describe the core’s deformation in the substrate part, and it is compatible with the displacement field of the core in the base part. In addition, capturing the high-order shear deformation of the core, this new theory is able to take the transverse compressibility and axial rigidity of the core into account. In this Part I of this two-part research, we focus on the formulation and the displacement field. In Part II, the fracture parameters, namely, the energy release rate and the mode mixity will be addressed. Accordingly, in this paper, results for the deformation of the debonded panel are produced and compared with the ones given by the finite element method with a very fine mesh. The accuracy is proven for a wide range of core materials and for a wide range of debond lengths.
Determination of Turbulent Prandtl Number for Thermal Fluid Dynamics Simulation of HVAC Unit by Data Assimilation
Authors: Liu Jun , Shimoyama Koji 
Published: August 2024

Abstract
Regarding high-precision temperature field prediction of a heating ventilation and air conditioning (HVAC) unit using thermal fluid dynamics simulation, the determination of the turbulent Prandtl number (Prt) was a key issue. In this study, we present an attempt to improve the accuracy of thermal fluid dynamics simulations in HVAC units by adjusting the Prt using data assimilation techniques. First, we simultaneously measured the velocity and temperature in the hot and cold air mixing zone of a simple HVAC model using particle image velocimetry and thermocouples. Second, we coupled data assimilation to the thermal fluid simulation model to determine the Prt in the mixing field. Finally, we proposed two functions of the Prt for high velocity and low velocity regions using multidimensional analysis. In the future, we believe that the Prt functions can be applied to thermal fluid simulations of actual HVAC unit to accurately predict performance without conducting prototype experiments, thereby contributing to reducing development cost and time of HVAC unit.
Chill Without Thrill: A Crossover Study on Whole-Body Cryotherapy and Postmatch Recovery in High-Level Youth Basketball Players
Authors: Pernigoni Marco , Perazzetti Andrea , Digno Mattia , Tessitore Antonio , Kamandulis Sigitas , Conte Daniele 
Published: August 2024

Purpose: To assess the effect of whole-body cryotherapy (WBC) on postmatch recovery in basketball. Methods: Using a crossover design, 17 youth male players (age 16.2 [1.2] y, stature 190.5 [9.4] cm, body mass 79.2 [9.6] kg, experience 9.9 [3.9] y) completed 2 simulated matches, followed by WBC (4 min, −75 to − 85 °C) or a placebo intervention (CON). Countermovement-jump height, change-of-direction performance, 10- and 20-m sprint times, heart-rate variability (log-transformed squared root of the mean sum of the squared differences between R-R intervals [Ln-rMSSD]), muscle soreness, and perceived recovery (Perceived Recovery Status Scale [PRS]) were recorded at prematch, postmatch, postrecovery, and 24 hours postmatch. Additionally, Ln-rMSSD was recorded upon awakening on match day and the following morning. Results: Compared with CON, higher PRS values were reported in WBC at prematch and postrecovery (P ≤ .026), while no significant between-interventions differences were found for any other measure (P > .05). Regarding the effect of time, our findings revealed that 20-m sprint times, Ln-rMSSD, and PRS deteriorated in both interventions from prematch to postmatch (ie, acute changes, P ≤ .045), while muscle soreness worsened in WBC only (P ≤ .003). Conversely, countermovement-jump height, change-of-direction, and 10-m sprint performance were unaffected by match play in the acute phase (P > .05), while none of the investigated measures showed impairments at 24 hours postmatch, compared with prematch (P > .05). Conclusions: Overall, these findings suggest that WBC was mostly ineffective for improving postexercise recovery in the investigated sample, with benefits observed for perceived recovery being potentially influenced by the participants’ status at baseline (ie, higher prematch PRS scores in WBC compared with CON).