bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Class of Heptaphyrins with NIR Absorption Modulated by Metal Coordination and Nucleophilic Substitution
Authors: Huang Yanping , Zhu Bin , Li Qizhao , Baryshnikov Glib , Li Chengjie , Sha Feng , Wu Xin‐Yan , Ågren Hans , Xie Yongshu 
Published: July 2024

AbstractThe intensive interest in expanded porphyrins can be attributed to their appealing photoelectric and coordination behavior. In this work, an N‐confused heptaphyrin 1 was synthesized by an acid‐catalyzed [3+4] condensation reaction. The introduction of an N‐confused pyrrolic unit into the heptaphyrin macrocycle led to the formation of a figure‐eight‐like conformation with nonsymmetrical “NNNN” and “NNNC” coordination cavities employable for bimetallic coordination. As a result, chelation of 1 with Zn(II) and Cu(II) afforded mono‐Zn(II) complex 2 and bis‐Cu(II) complex 3, respectively, with the metal atoms exhibiting distorted square‐planar geometries. In complex 3, an oxygen atom is attached to the α‐C atom of N‐confused pyrrole D, and thus the N and C atoms of ring D participate in coordination within the two cavities. Interestingly, treatment of 1 with Cs2CO3 in MeOH resulted in regioselective substitution of all the seven para‐F atoms in the meso‐C6F5 groups as well as the α‐H of ring D by eight methoxy moieties. Complex 3 displays a red‐shifted absorption band edge of ca. 2200 nm, compared to that of ca. 1600 nm observed for 1. This work provides an example of incorporating an N‐confused pyrrole to construct expanded porphyrins with distinctive coordination behavior and tunable NIR absorption.
Substrate and nutrient manipulation during continuous cultivation of extremophilic algae, Galdieria spp. RTK 37.1, substantially impacts biomass productivity and composition
Authors: Buckeridge Emma , Caballero Carlos C. , Smith Daniel H. , Stott Matthew B. , Carere Carlo R. 
Published: July 2024

AbstractThe extremophilic nature and metabolic flexibility of Galdieria spp. highlights their potential for biotechnological application. However, limited research into continuous cultivation of Galdieria spp. has slowed progress towards the commercialization of these algae. The objective of this research was to investigate biomass productivity and growth yields during continuous photoautotrophic, mixotrophic and heterotrophic cultivation of Galdieria sp. RTK371; a strain recently isolated from within the Taupō Volcanic Zone in Aotearoa‐New Zealand. Results indicate Galdieria sp. RTK371 grows optimally at pH 2.5 under warm white LED illumination. Photosynthetic O2 production was dependent on lighting intensity with a maximal value of (133.5 ± 12.1 nmol O2 mgbiomass−1 h−1) achieved under 100 μmol m−2 s−1 illumination. O2 production rates slowed significantly to 42 ± 1 and <0.01 nmol O2 mgbiomass−1 h−1 during mixotrophic and heterotrophic growth regimes respectively. Stable, long‐term chemostat growth of Galdieria sp. RTK371 was achieved during photoautotrophic, mixotrophic and heterotrophic growth regimes. During periods of ammonium limitation, Galdieria sp. RTK371 increased its intracellular carbohydrate content (up to 37% w/w). In contrast, biomass grown in ammonium excess was composed of up to 65% protein (w/w). Results from this study demonstrate that the growth of Galdieria sp. RTK371 can be manipulated during continuous cultivation to obtain desired biomass and product yields over long cultivation periods.
Reverse thinking: Tumor nutritional therapy
Authors: Ding Binbin , Ma Ping'an , Kheraif Abdulaziz A. Al , Lin Jun 
Published: July 2024

AbstractTumor cells often exhibit metabolic abnormalities to meet the needs of rapid proliferation, and targeting tumor metabolism has become one of the effective strategies for cancer treatment. However, most of the current methods targeting metabolism focus on inhibiting hyperactivated metabolic pathways, hindering their further application. A recent innovative work, proposed a nutrient‐based strategy to reactivate metabolism for tumor therapy by targeting suppressed metabolic pathways. This approach through delivering nutrients to tumor cells directly using nanotechnology indicates that specific nutrients can serve as potent activators of metabolic pathways. As a new direction along the reverse thinking, this study suggests that this nutrient‐based metabolism reactivation strategy will inspire broad applications in the treatment of other diseases associated with metabolic disorders, besides tumor.
Phase I study of TQB3602, an oral proteasome inhibitor, in relapsed and refractory multiple myeloma
Authors: Tang Wenjiao , Li Yan , Zhang Li , Zhong Xushu , Liang Qiushi , Zheng Yuhuan , Liu Yuzhang , Wang Yafei , Wang Xunqiang , Zeng Yun , Fang Baijun , Zheng Li , Niu Ting 
Published: July 2024

AbstractObjectiveTQB3602 is a novel orally bioavailable proteasome inhibitor. This study is the first‐in‐human phase I clinical trial to evaluate the safety, tolerability, pharmacokinetics, and preliminary efficacy of TQB3602 in relapsed/refractory multiple myeloma (RRMM).MethodsThis is a multicenter phase I clinical trial consisting of the 3+3 dose‐escalation phase and dose expansion phase. Patients with MM who have received ≥2 prior antimyeloma therapies were enrolled. TQB3602 is administered at a dose of 0.5~7mg on days 1, 8, 15 in 28‐day cycle.ResultsTwenty‐five RRMM patients who relapsed or failed ≥2 lines of therapies were enrolled in the dose escalation phase. Two patients in the 7.0 mg dose group developed dose‐limiting toxicity events (one with grade 2 peripheral neuropathy [PN] complicated by pain and one with diarrhea and abdominal pain), leading to a maximum tolerated dose of 6.0 mg. Any‐grade adverse events (AEs) occurred in 24 (96.0%) patients, while grade ≥3 AEs occurred in 13 (52.0%). The most common grade ≥3 AEs was anemia (6, 24.0%). The incidence rate of PN was 16% with no grade ≥3 PN occurred. TQB3602 was rapidly absorbed, resulting in a time‐to‐plasma peak concentration of 0.8–1.5 h. The mean half‐life was approximately 82 h. The AUClast and Cmax were approximately 1.9 times higher on day 15 than on day 1. Among 22 response‐evaluable patients, 63.7% achieved stable disease or better.ConclusionsTQB3602 is well tolerated, with a favorable neurotoxicity profile, and has shown preliminary efficacy in patients with RRMM. The anticipated therapeutic dose was 6 mg and was adopted for an ongoing dose‐expansion phase.
Synthesis, Antimicrobial ‐ Cytotoxic Evaluation, and Molecular Docking Studies of Quinolin‐2‐one Hydrazones Containing Nitrophenyl or Isonicotinoyl/Nicotinoyl Moiety
Authors: Doan Nam Q. H. , Tran Hoan N. , Nguyen Nhu T. M. , Pham Thu M. , Nguyen Quyen D. K. , Vu Thanh‐Thao 
Published: July 2024

AbstractBy applying the hybrid molecular strategy, in this study, we reported the synthesis of fifteen quinolin‐2‐one hydrazones containing nitrophenyl or nicotinonyl/isonicotinoyl moiety, followed by in vitro and in silico evaluations of their potential antimicrobial and anticancer activities. In vitro antimicrobial evaluation of the target compounds on seven pathogenic strains, applying the broth microdilution method, revealed that compound 4a demonstrated the most potential antifungal activity against C. albicans (MIC 512 μg mL−1) and C. krusei (MIC 128 μg mL−1). In vitro cytotoxic evaluation of the target compounds on three human cancer cell lines, employing the MTT method, suggested that compound 5c exhibited the most potential cytotoxicities against HepG2 (IC50 10.19 μM), A549 (IC50 20.43 μM), and MDA‐MB‐231 (IC50 16.82 μM) cells. Additionally, molecular docking studies were performed to investigate the binding characteristics of compounds 4a and 5c with fungal lanosterol 14α‐demethylase and human topoisomerase I–II, respectively, thereby contributing to the elucidation of their in vitro antifungal and cytotoxic properties. Furthermore, compounds 4a and 5c, via SwissADME prediction, could exhibit favorable physicochemical and pharmacokinetic properties. In conclusion, this study provides valuable insights into the potential of quinolin‐2‐one hydrazones as promising candidates for the development of novel antimicrobial and anticancer agents in the future.
A 64‐year‐old male with primary diffuse renal large B‐cell non‐Hodgkin lymphoma: A rare case report
Authors: Haydar Hasan , Sleiay Mouhammed , Alabdullah Hadi , Al‐alloush Rouba , Al_alloush Nour , Lutfi Mohamad Yasin , Youssef Simon , Hamsho Suaad 
Published: July 2024

Key Clinical MessageIn the context of lymphoma, it is of paramount importance to perform subsequent Positron Emission Tomography‐Computed Tomography (PET‐CT) scans to ensure the comprehensive eradication of neoplasms.AbstractPrimary renal diffuse tumors constitute less than 1% of all renal neoplasms. Among these, diffuse renal large B‐cell lymphoma is an exceedingly rare extranodal lymphoma. A 64‐year‐old male presented to the Department of Urology with complaints of persistent left flank discomfort for a duration of 2 weeks. Additionally, he reported generalized weakness, fatigue, and symptoms indicative of lower urinary tract obstruction, such as discomfort in the left testicle and dysuria. Ultrasound imaging revealed an echogenic structure with thickened, reactive walls and a turbid fluid core, located in the left flank, proximal to the lower pole of the kidney. This structure was subsequently identified as diffuse renal large B‐cell lymphoma. For the diagnosis of large B‐cell lymphomas, it is imperative that a proficient hematopathologist performs a comprehensive examination of the tumor tissue, preferably utilizing an excisional biopsy. The categorization of lymphoma requires specialized tests such as immunohistochemistry, flow cytometry, fluorescence in situ hybridization (FISH), and molecular testing. In instances where a renal mass is detected, healthcare professionals should consider performing a biopsy. In lymphoma cases, follow‐up Positron Emission Tomography‐Computed Tomography (PET‐CT) scans are crucial to confirm the complete eradication of the tumor.
Complex hidradenitis suppurativa on a background of long‐standing Crohn's disease requiring radical pelvic and perineal reconstruction: A case report
Authors: Forder Bea Harris , Nistor Sabina , Mykula Roman , Bignell Mark , Soleymani majd Hooman 
Published: July 2024

Key Clinical MessageA surgical MDT approach to high‐complexity surgeries can allow maximal resection in order to achieve disease control and excellent functional outcomes, as demonstrated here for a case of hidradenitis suppurativa in a patient with Crohn's disease.AbstractHidradenitis suppurativa is an autoimmune disease characterized by abscess and fistula formation with purulent discharge in intertriginous zones, and is associated with inflammatory bowel disease. We present the case of a patient with severe ongoing hidradenitis suppurativa causing osteomyelitis and affecting the perineum, on a background of Crohn's disease previously treated with panprotocolectomy and permanent ileostomy. The hidradenitis suppurativa was having a severe impact on the patient's quality of life, and she had failed to respond to conservative management. The patient opted for a radical two‐step procedure: first her coccyx and sacrum were removed. The second step was a radical bilateral anterior vulvectomy and posterior vaginectomy, with preservation of the uterine body and cervix. An anterolateral thigh flap was used to reconstruct the perineum. This complex procedure required the expertise of multiple surgical specialties, including plastic, general, spinal, and gynecological oncology surgeons to achieve maximal disease resection, minimizing the risk of recurrence.
Nondestructive Identification of Wheat Seed Variety and Geographical Origin Using Near‐Infrared Hyperspectral Imagery and Deep Learning
Authors: Sharma Apurva , Singh Tarandeep , Garg Neerja Mittal 
Published: July 2024

ABSTRACTSeed purity assurance is an important aspect of maintaining the quality standards of wheat seeds. It relies significantly on quality parameters, like varietal classification and geographical origin identification. Hyperspectral imaging (HSI) has emerged as an advanced nondestructive technique to determine various quality parameters. In recent years, several studies have utilized HSI for varietal classification, although a limited number of varieties were considered. Additionally, no attention has been paid to determining the geographical origin of wheat seeds. To address these gaps, two separate experiments were performed for varietal classification and geographical origin identification. The seeds from 96 varieties grown across 5 different agricultural regions in India were collected. Hyperspectral images of wheat seeds were acquired in the wavelength ranging 900–1700 nm. The spectral reflectance values were obtained from the region of interest (ROI) corresponding to each seed. Subsequently, the deep learning models (convolutional neural networks [CNNs]) were established and compared with two conventional algorithms, including support vector machines (SVMs) and K‐nearest neighbors (KNNs). The experimental results indicated that the proposed CNN models outperformed the SVM and KNN models, achieving an overall accuracy of 94.88% and 99.02% for varietal classification and geographical origin identification, respectively. These results demonstrate that HSI combined with deep learning has the potential to accurately classify a large number of wheat varieties. Moreover, HSI can be used to precisely identify the geographical origins of wheat seeds. This study provides an accurate and nondestructive method that can assist in breeding, quality evaluation, and the development of high‐quality wheat seeds.
Effect of Sodium−Glucose Co‐Transporter‐2 Inhibitor on Estimated Plasma Volume in a Patient With Heart Failure With Reduced Ejection Fraction and a Patient With Heart Failure With Preserved Ejection Fraction
Authors: Taťána Andreasová , Filip Málek 
Published: July 2024

ABSTRACTBackgroundThe increased diuresis after sodium−glucose cotransporter 2 inhibitor (SGLT2i) was associated with a reduction of the estimated plasma volume (ePV) in type 2 diabetic patients.HypothesisWe hypothesized that the early effect of SGLT2i on ePV may be monitored by the change of biomarkers of hemoconcentration.Patients and MethodsWe analyzed the early‐ and long‐term effect of SGLT2i empagliflozin on the ePV as assessed by biomarkers of hemoconcentration in a nondiabetic patient with heart failure and reduced ejection fraction (HFrEF) and a nondiabetic patient with heart failure and preserved ejection fraction (HFpEF). The ePV was calculated from hemoglobin and hematocrit levels by Duarte formula and ePV change was calculated by Strauss formula.ResultsThe ePV change was −22.56% between baseline and 1 month, and −37.60% between baseline and 12 months follow‐up in a patient with HFrEF, and −6.18% and −16.40% in a patient with HFpEF, respectively.ConclusionThe early effect of SGLT2i on ePV in patients with heart failure may be monitored by biomarkers of hemoconcentration.
Implementation of flow cytometry testing on rare matrix samples: Special considerations and best practices when the sample is unique or difficult to obtain
Authors: Devitt Katherine A. , Kern Wolfgang , Kajstura Malgorzata A. , Holl Eda K. , Hays Amanda L. , Hedley Benjamin D. , Gonneau Christèle , Jellison Evan R. , McCloskey Thomas W. , Mishra Shruti , Rebeles Jennifer , Ouseph Madhu M. 
Published: July 2024

AbstractThe publication of Clinical and Laboratory Standards Institute's guideline H62 has provided the flow cytometry community with much‐needed guidance on development and validation of flow cytometric assays (CLSI, 2021). It has also paved the way for additional exploration of certain topics requiring additional guidance. Flow cytometric analysis of rare matrices, or unique and/or less frequently encountered specimen types, is one such topic and is the focus of this manuscript. This document is the result of a collaboration subject matter experts from a diverse range of backgrounds and seeks to provide best practice consensus guidance regarding these types of specimens. Herein, we define rare matrix samples in the setting of flow cytometric analysis, address validation implications and challenges with these samples, and describe important considerations of using these samples in both clinical and research settings.
Reactive oxygen‐scavenging polydopamine nanoparticle coated 3D nanofibrous scaffolds for improved osteogenesis: Toward an aging in vivo bone regeneration model
Authors: Miszuk Jacob , Hu Jue , Wang Zhuozhi , Onyilagha Obiora , Younes Hammad , Hill Collin , Tivanski Alexei V. , Zhu Zhengtao , Sun Hongli 
Published: July 2024

AbstractTissue engineered scaffolds aimed at the repair of critical‐sized bone defects lack adequate consideration for our aging society. Establishing an effective aged in vitro model that translates to animals is a significant unmet challenge. The in vivo aged environment is complex and highly nuanced, making it difficult to model in the context of bone repair. In this work, 3D nanofibrous scaffolds generated by the thermally‐induced self‐agglomeration (TISA) technique were functionalized with polydopamine nanoparticles (PD NPs) as a tool to improve drug binding capacity and scavenge reactive oxygen species (ROS), an excessive build‐up that dampens the healing process in aged tissues. PD NPs were reduced by ascorbic acid (rPD) to further improve hydrogen peroxide (H2O2) scavenging capabilities, where we hypothesized that these functionalized scaffolds could rescue ROS‐affected osteoblastic differentiation in vitro and improve new bone formation in an aged mouse model. rPDs demonstrated improved H2O2 scavenging activity compared to neat PD NPs, although both NP groups rescued the alkaline phosphatase activity (ALP) of MC3T3‐E1 cells in presence of H2O2. Additionally, BMP2‐induced osteogenic differentiation, both ALP and mineralization, was significantly improved in the presence of PD or rPD NPs on TISA scaffolds. While in vitro data showed favorable results aimed at improving osteogenic differentiation by PD or rPD NPs, in vivo studies did not note similar improvements in ectopic bone formation an aged model, suggesting that further nuance in material design is required to effectively translate to improved in vivo results in aged animal models.
Development of a composite using calcined bone powder and silane cross‐linked alginate as bone substitute material
Authors: Tanaka Shigeo M. 
Published: July 2024

AbstractCalcined bone is an attractive natural material for use as a bone substitute because of its cost‐effectiveness and high biocompatibility, which are comparable to that of synthetic hydroxyapatite. However, the calcination process has significantly weakened the mechanical properties. In this study, a composite of calcined bovine bone powder reinforced with silane cross‐linked alginate was prepared to assess its biocompatibility, osteoconductivity, and mechanical compatibility as a bone substitute material. Culture studies with osteoblast‐like cells (MC3T3‐E1) showed no cytotoxicity toward the composite and exhibited general cell proliferative properties in its presence. In contrast, the composite reduced the alkaline phosphatase activity of osteoblasts but led to significant noncellular apatite deposition on the surface. In addition, quasi‐static compression tests of the composite revealed mechanical properties comparable to those of human cancellous bone. The mechanical properties remained stable under wet conditions and did not deteriorate significantly even after 2 weeks of immersion in simulated body fluid at 37°C. The results show that this composite, composed of calcined bone powder and silane cross‐linked alginate, is a promising bone substitute material with biocompatibility, osteoconductivity, and mechanical compatibility.
On the wing: Morphological variation in the osteology of Mediterranean, Near Eastern, and European Anatidae (excluding Anserinae)
Authors: Haruda Ashleigh , Mazzucato Camilla , Yeomans Lisa 
Published: July 2024

AbstractAccurate identification of waterfowl bones in archaeological and fossil assemblages has potential to unlock new methods of past environmental reconstruction, as species have differing habitat preferences and migration patterns. Therefore, identifying the presence of avian species with different ecological niches is key to determining past environments and ultimately how prehistoric people responded to climatic and environmental realignments. However, the identification of osteological remains of waterbirds such as ducks to species level is notoriously challenging. We address this by presenting a new two‐dimensional geometric morphometric protocol on wing elements from over 20 duck species and test the utility of these shape data for correct species identification. This is an ideal starting point to expand utilization of these types of approaches in avifaunal research and test applicability to an extremely difficult taxonomic group.
Interannual variability of the occurrence frequency of the stronger Kunming quasi‐stationary front in winter
Authors: Du Xiaorui , Dai Haocheng , Cao Jie , Tao Yun , Yu Jiantong 
Published: July 2024

AbstractUsing ERA5 reanalysis data, this study explores the interannual variability of the occurrence frequency of the stronger Kunming quasi‐stationary front during in winter over the period 1980–2021 and the main causes of the variability. The results indicate that the occurrence frequency is significantly modulated on interannual timescales by southwesterly anomalies over the warmer region west of 103° E and northeasterly anomalies over the colder region east of 106° E. When the stronger southwesterly anomalies over the warmer region are comparable with stronger northeasterly anomalies over the colder region, the inhomogeneities of horizontal convergence and diabatic heating are strengthened near the frontal zone. This anomalous circulation causes a higher‐than‐normal occurrence frequency of the stronger Kunming quasi‐stationary front in winter. The East Asian winter monsoon (EAWM) and circumglobal teleconnection (CGT) are the two most important controls on the interannual variability of the occurrence frequency of the stronger Kunming quasi‐stationary front. Both the CGT and EAWM modulate the inhomogeneities of horizontal convergence and diabatic heating on the west flank of the front, and the EAWM further regulates diabatic heating over the east flank of the front.
Utilization and impact of esophageal string testing in children with eosinophilic esophagitis: A 1 year experience
Authors: Quinn Laura A. , Andrews Rachel , Bauer Maureen , Nguyen Nathalie 
Published: July 2024

AbstractThe 1‐h esophageal string test (EST) is a minimally invasive test that can be used to monitor eosinophilic esophagitis (EoE) disease activity and guide treatment without endoscopy. We aimed to describe the real‐world utilization and impact of EST on the care of children with EoE over the first year this was used at our center. Between 12/1/2022 and 11/30/2023, 39 ESTs were successful in 45 attempts (87% completion rate) in 31 patients. Five patients underwent multiple ESTs. Adverse events during the EST included vomiting. Reasons for failure to complete the EST (13%, n = 6) were patients could not swallow the capsule (n = 5) and vomiting (n = 1). EST was used to assess EoE without the need for endoscopy in 95% (n = 37) of cases. Treatment approach varied based on whether the EST indicated active (38.5%) or inactive (61.5%) EoE. The EST is a well‐tolerated minimally invasive disease monitoring tool for patients with EoE.