bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structuring the peer assessment process: a multilevel approach for the impact on product improvement and peer feedback quality
Authors: Gielen M. , De Wever B. 
Published: January 2015

AbstractIn order to optimize students' peer feedback processes, this study investigates how an instructional intervention in the peer assessment process can have a beneficial effect on students' performance in a wiki environment in first‐year higher education. The main aim was to study the effect of integrating a peer feedback template with a varying structuring degree. The present study involved three conditions: a no structure, a basic structure and an elaborate structure condition. Due to a clear hierarchical structure, in which over time (level 1), 168 students (level 2) are nested within 37 groups (level 3), multilevel analysis was performed to examine the effect of time, student and group level influences on students' peer feedback quality and product scores. The results revealed that both peer feedback quality and product scores increase significantly for all conditions over time, after multiple practice occasions. In addition, after several practice occasions, significant differences were found between the conditions in both peer feedback (elaborate higher than no structure) and product scores (elaborate and basic higher than no structure). Building on this, limitations, directions for future research and practical implications are presented.
Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin‐resistant uterine cancer
Authors: Lo Yi‐Wen , Lin Szu‐Ting , Chang Shing‐Jyh , Chan Chia‐Hao , Lyu Kevin W. , Chang Jo‐Fan , May Eugenie Wong Soon , Lin Dai‐Ying , Chou Hsiu‐Chuan , Chan Hong‐Lin 
Published: January 2015

AbstractMitochondria are key organelles in mammary cells in responsible for a number of cellular functions including cell survival and energy metabolism. Moreover, mitochondria are one of the major targets under doxorubicin treatment. In this study, low‐abundant mitochondrial proteins were enriched for proteomic analysis with the state‐of‐the‐art two‐dimensional differential gel electrophoresis (2D‐DIGE) and matrix‐assistant laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) strategy to compare and identify the mitochondrial protein profiling changes in response to the development of doxorubicin resistance in human uterine cancer cells. The mitochondrial proteomic results demonstrate more than fifteen hundred protein features were resolved from the equal amount pooled of three purified mitochondrial proteins and 101 differentially expressed spots were identified. In which, 39 out of these 101 identified proteins belong to mitochondrial proteins. Mitochondrial proteins such as acetyl‐CoA acetyltransferase (ACAT1) and malate dehydrogenase (MDH2) have not been reported with the roles on the formation of doxorubicin resistance in our knowledge. Further studies have used RNA interference and cell viability analysis to evidence the essential roles of ACAT1 and MDH2 on their potency in the formation of doxorubicin resistance through increased cell viability and decreased cell apoptosis during doxorubicin treatment. To sum up, our current mitochondrial proteomic approaches allowed us to identify numerous proteins, including ACAT1 and MDH2, involved in various drug‐resistance‐forming mechanisms. Our results provide potential diagnostic markers and therapeutic candidates for the treatment of doxorubicin‐resistant uterine cancer.
Functionalization of titanium implants using a modular system for binding and release of VEGF enhances bone‐implant contact in a rodent model
Authors: Schliephake Henning , Rublack Jennifer , Förster Anne , Schwenzer Bernd , Reichert Judith , Scharnweber Dieter 
Published: January 2015

AbstractAimsTo test the immobilization of vascular endothelial growth factor (VEGF165) on the surface of titanium implants using DNA oligonucleotide (ODN) anchor strands for the ability to enhance periimplant bone formation.Materials and MethodsDNA oligonucleotides were anchored to the surface of sandblasted acid‐etched (SAE) titanium screw implants and were hybridized with complementary strands of ODN conjugated to rhVEGF165. The implants were tested against blank SAE implants and SAE implants with nano‐anchored ODN. The implants were inserted into the tibiae of 36 Sprague–Dawley rats. Primary outcome parameters were bone‐implant contact (BIC), amount of new bone formation and periimplant bone density (BD). density after 1, 4 and 13 weeks. Unit of analysis has been the individual implant.ResultsImplants with rhVEGF165 hybridized to ODN anchor strands exhibited significantly increased average BIC after 1 month compared to blank implants and implants with anchored ODN strands.ConclusionsIt is concluded that rhVEGF165immobilized on the surface of titanium implants through nano‐anchored oligonucleotide strands can accelerate BIC of sandblasted and etched titanium implants to a certain extent. The radius of effect of the growth factor appears to be limited to tissue immediately adjacent to the implant surface.
Impact of extracorporeal shockwave therapy on tooth mobility in adult orthodontic patients: a randomized single‐center placebo‐controlled clinical trial
Authors: Falkensammer Frank , Rausch‐Fan Xiaohui , Schaden Wolfgang , Kivaranovic Danijel , Freudenthaler Josef 
Published: January 2015

AbstractAimThis RCT investigated the effect of non‐invasive extracorporeal shockwaves on tooth mobility in orthodontic patients after active treatment.Materials and methodsSeventy‐two adult patients were included in the study. Immediately after active orthodontic treatment, patients were assigned to a treatment or a placebo group based on block randomization. The orthodontic patients were required to be otherwise healthy. The region of interest was the anterior portion of the mandible. The treatment group received a single shockwave treatment with 1000 impulses while the placebo group was treated with an acoustic sham. Tooth mobility was evaluated over a period of 6 months using a Periotest and manual testing. Pocket probing depths, bleeding on probing and the irregularity index were also assessed.ResultsTooth mobility reduced significantly over 6 months in both groups, but shockwaves achieved significantly more rapid reduction on manual testing. Probing depth was significantly reduced while the irregularity index remained stable. Bleeding on probing was significantly reduced in the treatment group. No anti‐inflammatory effect could be derived due to possible initial group differences.ConclusionsThe mobility of teeth aligned by orthodontic treatment reduces over time. Shockwave treatment appeared to reduce tooth mobility more rapidly.
Establishment of maturity‐onset diabetes of the young‐induced pluripotent stem cells from a Japanese patient
Authors: Yabe Shigeharu G , Iwasaki Naoko , Yasuda Kazuki , Hamazaki Tatsuo S , Konno Masamitsu , Fukuda Satsuki , Takeda Fujie , Kasuga Masato , Okochi Hitoshi 
Published: January 2015

AbstractMaturity‐onset diabetes of the young (MODY) is a heterozygous monogenic diabetes; more than 13 disease genes have been identified. However, the pathogenesis of MODY is not fully understood, because the pancreatic β‐cells of the patients are inaccessable. Therefore, we attempted to establish MODY patient‐derived induced pluripotent stem cells (MODY‐iPS) cells to investigate the pathogenic mechanism of MODY by inducing pancreatic β‐cells. We established MODY5‐iPS cells from a Japanese patient with MODY5 (R177X), and confirmed that MODY5‐iPS cells possessed the characteristics of pluripotent stem cells. In the course of differentiation from MODY5‐iPS cells into pancreatic β‐cells, we examined the disease gene, HNF1B messenger ribonucleic acid. We found that the amount of R177X mutant transcripts was much less than that of wild ones, but they increased after adding cycloheximide to the medium. These results suggest that these R177X mutant messenger ribonucleic acids are disrupted by nonsense‐mediated messenger ribonucleic acid decay in MODY‐iPS cells during the developmental stages of pancreatic β‐cells.
Corneal confocal microscopy: Recent progress in the evaluation of diabetic neuropathy
Authors: Papanas Nikolaos , Ziegler Dan 
Published: January 2015

AbstractThe present brief review discusses recent progress with corneal confocal microscopy for the evaluation of diabetic sensorimotor polyneuropathy. Corneal confocal microscopy is a new, non‐invasive and reproducible diagnostic modality, and it can also be easily applied for patient follow up. It enables new perspectives of studying the natural history of diabetic sensorimotor polyneuropathy, severity of nerve fiber pathology and documenting early nerve fiber regeneration after therapeutic intervention. It shows moderate to high sensitivity and specificity for the timely diagnosis of diabetic sensorimotor polyneuropathy. Currently, corneal confocal microscopy is mainly used in specialized centers, but deserves more widespread application for the assessment of diabetic sensorimotor polyneuropathy. Finally, further progress is required in terms of technical improvements for automated nerve fiber quantification and for analysis of larger images.
Preliminary Evidence That Bioactive Cements Occlude Artificial Marginal Gaps
Authors: Jefferies Steven R. , Fuller Alexander E. , Boston Daniel W. 
Published: January 2015

AbstractPurposeThe objective of this preliminary laboratory study was to explore whether bioactive dental cements have the ability to seal marginal gaps as compared with other classes of dental cements.Materials and MethodsTooth discs (dentin/enamel) were positioned adjacent to a second disc of one of five possible dental cements (two bioactive and three conventional) and separated by a 50‐μm Mylar film, creating a controlled gap between the two discs. The edges of the tooth segments were abraded to expose dentin such that the dentin interface was positioned opposite the cement. The artificial gap assembly was stabilized with adhesive and composite resin. Artificial gap assemblies for each cement type were immersed in a phosphate‐containing, simulated body fluid (phosphate buffered saline) for up to 8 months. Occlusion of artificial marginal gaps (including deposition of crystal‐like, mineralized deposits within the gaps) was observed and scored categorically during the observation period. Deposition of radiodense material within the artificial marginal gap space was confirmed by micro‐computerized tomography analysis.ResultsThere was no evidence of marginal gap occlusion for the three conventional control cements, whereas both bioactive, surface apatite‐forming cements demonstrated occlusion of the artificial marginal gaps.ConclusionsThe preliminary findings of this initial study suggest the possible capability of surface apatite‐forming, calcium‐based, bioactive dental cements to seal or reseal artificial marginal gaps in simulated aqueous physiological conditions.Clinical SignificanceThe results of this laboratory study, although preliminary in nature, suggest a new functional property for bioactive dental materials, namely the ability to significantly improve marginal stability with tooth/restorative material interface. Such behavior in a restorative material could improve the survival and serviceability of dental restorations.
Structural Properties and Mechanical Durability of Extruded Fish Feed
Authors: Haubjerg A.F. , Veje C.T. , Jørgensen B.N. , Simonsen B. , Løvgreen S. 
Published: January 2015

AbstractThis article investigates the possible correlation between mechanical properties of fish feed pellets and their mechanical durability. Mechanical properties were obtained by texture profile analysis (TPA) and stress relaxation test (SRT) of different types of fish feed. The results were correlated against a conventional test of mechanical durability (DORIS, Durability On a Realistic, test). From SRT it was found that for achieving a high durability, pellets should be able to relax an applied force nonelastically. From TPA, it was found that a durable pellet should also be able to return its original state after a deformation. By fitting SRT data with a two time constant generalized Maxwell model, it was found that the largest time constant is ∼7 s. The waiting time in‐between the two impacts in TPA was increased from 5 to 15 s, which significantly improved the correlations against mechanical durability.Practical ApplicationsThe results indicate that a waiting time of more than ∼7 s will allow the extruded fish feed pellet to relax to its equilibrium state. This can be readily implemented in the texture profile analysis method to increase the response in correlations between structural properties and mechanical durability. The specific results presented in this article indicate that the viscoelastic behavior of the pellets should be controlled to increase mechanical durability. The realizations from this work will benefit future research aiming to promote mechanical durability of fish feed pellets in the drying process. The concept and findings will however find use in the assessment and promotion of mechanical durability for a much wider range of dry feed and food products.
Medication Misadventures in Older Adults: Literature from 2013
Authors: Hanlon Joseph T. , Semla Todd P. , Schmader Kenneth E. 
Published: January 2015

The objective of this paper is to review articles published in 2013 examining drug‐related problems in the elderly and comment on their potential impact on clinical practice. To identify articles, we did a systematic search of the English‐language literature restricted to those aged 65 + from January 2013 to December 2013 using Medline and Google Scholar and a combination of the following search terms: drug‐related problems, medication‐related problems, medication errors, suboptimal prescribing, inappropriate prescribing, underutilization, polypharmacy, medication monitoring, medication dispensing, medication administration, medication adherence, adverse drug events, and adverse drug withdrawal events. A manual search of major general medicine and clinical pharmacology journals was also conducted to identify additional articles. A total of 51 articles were identified of which 20 were chosen to highlight. Three were annotated and critiqued and the additional 17 articles were summarized in an appendix. One article reported the results of a randomized controlled trial that showed that a pharmacist intervention successfully reduced suboptimal prescribing in older hospital patients. Another paper from this group previously reported data from the same study showing that the intervention also reduced medication related readmissions to the hospital. An observational study compared the use of two thiazide diuretics in older outpatients. They found that chlorthalidone was more likely to cause hypokalemia than hydrochlorothiazide. Finally, in a randomized controlled trial a pharmacist intervention resulted in the reduction of anticholinergic burden but did result in an improvement in cognition. These studies highlight that medication errors and adverse drug events continue to be important issues for health care professionals caring for older adults.
Expressing an (E)‐β‐farnesene synthase in the chloroplast of tobacco affects the preference of green peach aphid and its parasitoid
Authors: Wang Gen‐Ping , Yu Xiu‐Dao , Fan Jia , Wang Cheng‐She , Xia Lan‐Qin 
Published: January 2015

Abstract(E)‐β‐Farnesene (EβF) synthase catalyses the production of EβF, which for many aphids is the main or only component of the alarm pheromone causing the repellence of aphids and also functions as a kairomone for aphids' natural enemies. Many plants possess EβF synthase genes and can release EβF to repel aphids. In order to effectively recruit the plant‐derived EβF synthase genes for aphid control, by using chloroplast transit peptide (CTP) of the small subunit of Rubisco (rbcS) from wheat (Triticum aestivum L.), we targeted AaβFS1, an EβF synthase gene from sweet wormwood (Artemisia annua L.), to the chloroplast of tobacco to generate CTP + AaβFS1 transgenic lines. The CTP + AaβFS1 transgenic tobacco plants could emit EβF at a level up to 19.25 ng/day per g fresh tissues, 4–12 fold higher than the AaβFS1 transgenic lines without chloroplast targeting. Furthermore, aphid/parasitoid behavioral bioassays demonstrated that the CTP + AaβFS1 transgenic tobacco showed enhanced repellence to green peach aphid (Myzus persicae) and attracted response of its parasitoid Diaeretiella rapae, thus affecting aphid infestation at two trophic levels. These data suggest that the chloroplast is an ideal subcellular compartment for metabolic engineering of plant‐derived EβF synthase genes to generate a novel type of transgenic plant emitting an alarm pheromone for aphid control.
Successful ex utero intrapartum treatment procedure for prenatally diagnosed severe micrognathia: A case report
Authors: Suenaga Masanori , Hidaka Nobuhiro , Kido Saki , Otera Yuka , Fukushima Kotaro , Kato Kiyoko 
Published: January 2015

AbstractWe present a case of fetal severe micrognathia in which successful airway stabilization was achieved by an ex utero intrapartum treatment procedure. In this case, it was anticipated that the infant would have a vulnerable airway at birth based on in utero sonographic findings, including an extremely hypoplastic jaw, worsening polyhydramnios and absence of stomach visualization. Early sonographic recognition was helpful in preparing the parents and physicians for the possibility of airway emergencies during the perinatal period. When a severely hypoplastic mandible accompanied by polyhydramnios and absent stomach visualization is noted on ultrasound, clinicians should consider the indication for ex utero intrapartum treatment. A multidisciplinary team with technically skilled medical providers should be coordinated to perform the procedure.
Case of concurrent benign metastasizing leiomyoma in the lung and retroperitoneum, with a focus on its etiology
Authors: Tohya Toshimitsu , Tajima Tomotaka , Takeshita Yuko , Ito Kiyotaka , Kuriwaki Kazumi , Katabuchi Hidetaka 
Published: January 2015

AbstractWe report a rare, simultaneous occurrence of benign metastasizing leiomyoma in the lung and retroperitoneum in a 49‐year‐old woman who had previously undergone myomectomy at 35 years of age and hysterectomy at 45 years of age for multiple recurrences of histologically benign uterine leiomyomas. At 49 years of age, computed tomography‐guided biopsy indicated benign metastasizing leiomyomas in the lung. In addition, a retroperitoneal leiomyoma was found that was resected along with both the ovaries via laparotomy. No sign or symptom of recurrence was observed 5 years later. The coexistence of benign metastasizing leiomyoma in the lung and retroperitoneum following surgery for conventional leiomyomas has rarely been reported. Further, the nature and etiology of benign metastasizing leiomyoma are still not well understood. This case is therefore worth reporting, and exploring its etiology is important.
Thalidomide affects experimental endometriosis: A randomized controlled study in the rat
Authors: Azimirad Afshin , Alborzi Saeed , Kumar Perikala Vijayananda , Zolghadri Jaleh , Zarei Afsoon , Tavana Zohreh , Azimirad Mina 
Published: January 2015

AbstractAimThis study aimed to assess the efficacy of thalidomide for treatment of experimental endometriosis.MethodsThis study was a parallel‐group, double‐blind, stratified, randomized controlled animal trial with 1:1 allocation ratio. Endometriosis was induced experimentally in 23 mature, nulligravid, female Sprague–Dawley rats, weighing approximately 200 g and aged 2 months. A checkpoint surgery was performed 6 weeks later. Then, the rats were randomly allocated into the thalidomide (22 mg/day p.o.) and control (0.5 mL saline 0.9%/day p.o.) groups of nine. After 6 weeks, they were killed. Before each laparotomy, blood for leukocyte and lymphocyte counts and during them, implants for histopathology and peritoneal fluid for interleukin (IL)‐6, tumor necrosis factor‐α and vascular endothelial growth factor (VEGF)‐A concentrations (by enzyme‐linked immunoassay) were collected. Allocation and stratified randomization were done using a computer, based on the obtained histopathology scores of the implants of the checkpoint surgery.ResultsThe histopathology scores (the main outcome measure) were 2.00 ± 1.55 versus 0.44 ± 1.01 (P = 0.035). The comparisons of after‐treatment counts of leukocytes, lymphocytes, VEGF‐A and IL‐6 between the two groups were statistically significant.ConclusionThe results of this study are in favor of therapeutic implication of thalidomide in experimental endometriosis in rats. This is the first time thalidomide has been evaluated on endometriosis in an animal model.
Acupuncture for the Treatment of Vulvodynia: A Randomized Wait-List Controlled Pilot Study
Authors: Schlaeger Judith M. , Xu Nenggui , Mejta Cheryl L. , Park Chang G. , Wilkie Diana J. 
Published: January 2015

Abstract

Introduction
The incidence of vulvodynia in American women has been reported to be between 8.3% and 16%. However, there is no consistently effective standardized treatment for vulvodynia.

Aim
To determine the feasibility and potential effects of using a standardized acupuncture protocol for the treatment of women with vulvodynia.

Main Outcome Measures
The primary outcome was vulvar pain, and sexual function was the secondary outcome. Pain was assessed by the Short-Form McGill Pain Questionnaire, and function was measured by the Female Sexual Function Index (FSFI).

Methods
Thirty-six women with vulvodynia met inclusion criteria. The women were randomly assigned either to the acupuncture group or to the wait-list control group. The 18 subjects assigned to the acupuncture group received acupuncture two times per week for 5 weeks for a total of 10 sessions.

Results
Reports of vulvar pain and dyspareunia were significantly reduced, whereas changes in the aggregate FSFI scores suggest significant improvement in sexual functioning in those receiving acupuncture vs. those who did not. Acupuncture did not significantly increase sexual desire, sexual arousal, lubrication, ability to orgasm or sexual satisfaction in women with vulvodynia.

Conclusion
This was the first randomized controlled pilot study to examine the use of acupuncture for the treatment of vulvodynia. The acupuncture protocol was feasible and in this small sample appeared to reduce vulvar pain and dyspareunia with an increase in overall sexual function for women with vulvodynia. This study should be replicated in a larger double-blinded randomized controlled trial.