bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extensive localization of long noncoding RNAs to the cytosol and mono- and polyribosomal complexes
Authors: van Heesch Sebastiaan , van Iterson Maarten , Jacobi Jetse , Boymans Sander , Essers Paul B , de Bruijn Ewart , Hao Wensi , MacInnes Alyson W , Cuppen Edwin , Simonis Marieke 
Published: January 2014

Abstract
Background
Long noncoding RNAs (lncRNAs) form an abundant class of transcripts, but the function of the majority of them remains elusive. While it has been shown that some lncRNAs are bound by ribosomes, it has also been convincingly demonstrated that these transcripts do not code for proteins. To obtain a comprehensive understanding of the extent to which lncRNAs bind ribosomes, we performed systematic RNA sequencing on ribosome-associated RNA pools obtained through ribosomal fractionation and compared the RNA content with nuclear and (non-ribosome bound) cytosolic RNA pools.

Results
The RNA composition of the subcellular fractions differs significantly from each other, but lncRNAs are found in all locations. A subset of specific lncRNAs is enriched in the nucleus but surprisingly the majority is enriched in the cytosol and in ribosomal fractions. The ribosomal enriched lncRNAs include H19 and TUG1.

Conclusions
Most studies on lncRNAs have focused on the regulatory function of these transcripts in the nucleus. We demonstrate that only a minority of all lncRNAs are nuclear enriched. Our findings suggest that many lncRNAs may have a function in cytoplasmic processes, and in particular in ribosome complexes.
RIP-seq analysis of eukaryotic Sm proteins identifies three major categories of Sm-containing ribonucleoproteins
Authors: Lu Zhipeng , Guan Xiaojun , Schmidt Casey A , Matera A Gregory 
Published: January 2014

Abstract
Background
Sm proteins are multimeric RNA-binding factors, found in all three domains of life. Eukaryotic Sm proteins, together with their associated RNAs, form small ribonucleoprotein (RNP) complexes important in multiple aspects of gene regulation. Comprehensive knowledge of the RNA components of Sm RNPs is critical for understanding their functions.

Results
We developed a multi-targeting RNA-immunoprecipitation sequencing (RIP-seq) strategy to reliably identify Sm-associated RNAs from Drosophila ovaries and cultured human cells. Using this method, we discovered three major categories of Sm-associated transcripts: small nuclear (sn)RNAs, small Cajal body (sca)RNAs and mRNAs. Additional RIP-PCR analysis showed both ubiquitous and tissue-specific interactions. We provide evidence that the mRNA-Sm interactions are mediated by snRNPs, and that one of the mechanisms of interaction is via base pairing. Moreover, the Sm-associated mRNAs are mature, indicating a splicing-independent function for Sm RNPs.

Conclusions
This study represents the first comprehensive analysis of eukaryotic Sm-containing RNPs, and provides a basis for additional functional analyses of Sm proteins and their associated snRNPs outside of the context of pre-mRNA splicing. Our findings expand the repertoire of eukaryotic Sm-containing RNPs and suggest new functions for snRNPs in mRNA metabolism.
Phase III Randomized Trial of Weekly Cisplatin and Irradiation Versus Cisplatin and Tirapazamine and Irradiation in Stages IB2, IIA, IIB, IIIB, and IVA Cervical Carcinoma Limited to the Pelvis: A Gynecologic Oncology Group Study
Authors: DiSilvestro Paul A. , Ali Shamshad , Craighead Peter S. , Lucci Joseph A. , Lee Yi-Chun , Cohn David E. , Spirtos Nicola M. , Tewari Krishnasu S. , Muller Carolyn , Gajewski Walter H. , Steinhoff Margaret M. , Monk Bradley J. 
Published: January 2014

PurposeThis prospective, randomized phase III intergroup trial of the Gynecologic Oncology Group and National Cancer Institute of Canada Clinical Trials Group was designed to test the effectiveness and safety of adding the hypoxic cell sensitizer tirapazamine (TPZ) to standard cisplatin (CIS) chemoradiotherapy in locally advanced cervix cancer.Patients and MethodsPatients with locally advanced cervix cancer were randomly assigned to CIS chemoradiotherapy versus CIS/TPZ chemoradiotherapy. Primary end point was progression-free survival (PFS). Secondary end points included overall survival (OS) and tolerability.ResultsPFS was evaluable in 387 of 402 patients randomly assigned over 36 months, with enrollment ending in September 2009. Because of the lack of TPZ supply, the study did not reach its original target accrual goal. At median follow-up of 28.3 months, PFS and OS were similar in both arms. Three-year PFS for the TPZ/CIS/RT and CIS/RT arms were 63.0% and 64.4%, respectively (log-rank P = .7869). Three-year OS for the TPZ/CIS/RT and CIS/RT arms were 70.5% and 70.6%, respectively (log-rank P = .8333). A scheduled interim safety analysis led to a reduction in the starting dose for the TPZ/CIS arm, with resulting tolerance in both treatment arms.ConclusionTPZ/CIS chemoradiotherapy was not superior to CIS chemoradiotherapy in either PFS or OS, although definitive commentary was limited by an inadequate number of events (progression or death). TPZ/CIS chemoradiotherapy was tolerable at a modified starting dose.
Differential operators on quantized flag manifolds at roots of unity, II
Authors: Tanisaki Toshiyuki 
Published: January 2014

AbstractWe formulate a Beilinson-Bernstein-type derived equivalence for a quantized enveloping algebra at a root of 1 as a conjecture. It says that there exists a derived equivalence between the category of modules over a quantized enveloping algebra at a root of 1 with fixed regular Harish-Chandra central character and the category of certain twistedD-modules on the corresponding quantized flag manifold. We show that the proof is reduced to a statement about the (derived) global sections of the ring of differential operators on the quantized flag manifold. We also give a reformulation of the conjecture in terms of the (derived) induction functor.
Effect of chromosome substitution on intrinsic exercise capacity in mice
Authors: Courtney Sean M. , Massett Michael P. 
Published: January 2014

Previous research identified a locus on Chromosome 14 as an important regulator of endurance exercise capacity in mice. The aim of this study was to investigate the effect of chromosome substitution on intrinsic exercise capacity and identify quantitative trait loci (QTL) associated with exercise capacity in mice. Mice from a chromosome substitution strain (CSS) derived from A/J and C57Bl/6J (B6), denoted as B6.A14, were used to assess the contribution of Chromosome 14 to intrinsic exercise capacity. All mice performed a graded exercise test to exhaustion to determine exercise capacity expressed as time (min) or work (kg·m). Exercise time and work were significantly greater in B6 mice than B6.A14 and A/J mice, indicating the presence of a QTL on Chromosome 14 for exercise capacity. To localize exercise-related QTL, 155 B6.A14 x B6 F2mice were generated for linkage analysis. Suggestive QTL for exercise time (57 cM, 1.75 LOD) and work (57 cM, 2.08 LOD) were identified in the entire B6.A14 x B6 F2cohort. To identify putative sex-specific QTL, male and female F2cohorts were analyzed separately. In males, a significant QTL for exercise time (55 cM, 2.28 LOD) and a suggestive QTL for work (55 cM, 2.19 LOD) were identified. In the female cohort, no QTL was identified for time, but a suggestive QTL for work was located at 16 cM (1.8 LOD). These data suggest that one or more QTL on Chromosome 14 regulate exercise capacity. The putative sex-specific QTL further suggest that the genetic architecture underlying exercise capacity is different in males and females. Overall, the results of this study support the use of CSS as a model for the genetic analysis of exercise capacity. Future studies should incorporate the full panel of CSS using male and female mice to dissect the genetic basis for differences in exercise capacity.
Mind and media: Exploring the Freud-McLuhan connection
Authors: Braga Adriana , Logan Robert K. 
Published: January 2014

Abstract
In this article we examine the thesis that Sigmund Freud might have had a possible influence on the thinking of Marshall McLuhan. We also develop the parallels in their thinking. The first hint of this connection is that McLuhan frequently refers to Freud in his writings. The second hint is that both men were battling invisible forces - unnoticed or subliminal effects of media for McLuhan and repressed memories and the unconscious for Freud. We present this hypothesis as a probe, which we believe has some degree of truth to it given the frequency with which McLuhan referred to and quoted Freud. Even if it is not true it is still illuminating to see the parallels of these two revolutionary thinkers who explored the workings of the human psyche from two completely different perspectives.
“Preflight Checklists” for diagnosis: a personal experience
Authors: Ely John W. 
Published: January 2014

AbstractAs physicians, we take pride in our ability to generate, from memory, a complete differential diagnosis for our patients’ presenting symptoms. We expect this of ourselves and our trainees, but we do not do it reliably. Studies have found that the most common cause of diagnostic error is the physician’s failure to consider the correct diagnosis as a possibility. Other professionals, like airline pilots and nuclear plant operators, have accepted the fallibility of their memories and have learned how to ensure reliable completion of critical tasks by using checklists. But our culture in medicine glorifies physicians who complete the critical task of diagnosis using their memories and disparages those who cheat by referring to a list. Recent studies have supported the use of checklists in the operating room and intensive care unit, but so far they have not been used to make diagnosis more reliable. This essay explores a possible use for differential-diagnosis checklists by describing the author’s experience with them in a primary care clinic.
Radiologic errors, past, present and future
Authors: Berlin Leonard 
Published: January 2014

AbstractDuring the 10-year period beginning in 1949 with publication of five articles in two radiology journals and UKs The Lancet, a California radiologist named L.H. Garland almost single-handedly shocked the entire medical and especially the radiologic community. He focused their attention on the fact now known and accepted by all, but at that time not previously recognized and acknowledged only with great reluctance, that a substantial degree of observer error was prevalent in radiologic interpretation. In the more than half-century that followed, Garland’s pioneering work has been affirmed and reaffirmed by numerous researchers. Retrospective studies disclosed then and still disclose today that diagnostic errors in radiologic interpretations of plain radiographic (as well as CT, MR, ultrasound, and radionuclide) images hover in the 30% range, not too dissimilar to the error rates in clinical medicine. Seventy percent of these errors are perceptual in nature, i.e., the radiologist does not “see” the abnormality on the imaging exam, perhaps due to poor conspicuity, satisfaction of search, or simply the “inexplicable psycho-visual phenomena of human perception.” The remainder are cognitive errors: the radiologist sees an abnormality but fails to render a correct diagnoses by attaching the wrong significance to what is seen, perhaps due to inadequate knowledge, or an alliterative or judgmental error. Computer-assisted detection (CAD), a technology that for the past two decades has been utilized primarily in mammographic interpretation, increases sensitivity but at the same time decreases specificity; whether it reduces errors is debatable. Efforts to reduce diagnostic radiological errors continue, but the degree to which they will be successful remains to be determined.
Connecting the dots: like watching a movie…critically
Authors: Heudebert Gustavo R. 
Published: January 2014

AbstractAcquisition of expertise in the diagnosis and management of patients requires years of practice; exposure to diverse clinical entities is critical as well as the myriad ways in which the same disease can present in a given patient. However, this repeated exposure has to be accompanied by two critical elements; first, the novice needs the guidance of an expert or master to appreciate the nuances and subtleties in making a diagnosis or taking a course of action. Second, and perhaps most importantly, the physician needs to acquire the habit of reflecting on and in actions during the clinical encounter. Unguided repetition during formative years or lack of critical introspection during practice hinders the progression to expertise. In a way, a complex clinical encounter is akin to watching a complex movie; it takes repeated exposure to the movie to understand the subtleties the director is utilizing to understand the plot in its entirety.
The critical step to reduce diagnostic errors in medicine: addressing the limitations of human information processing
Authors: Zwaan Laura 
Published: January 2014

AbstractOver the last 50 years diagnostic testing has improved dramatically and we are now able to diagnose patients faster and more precisely than ever before. However, the incidence of diagnostic errors, particularly of common diseases, has remained relatively stable over time. In this paper, I argue that the intrinsic limitations of human information processing are crucial. The way people process information has not changed over the years and is the main cause of diagnostic error. To take a decisive step forward and substantially reduce the number of diagnostic errors in medicine, we need to create an environment which takes the intrinsic limitations of in human information processing into account.
The diagnostic field’s players and interactions: from the inside out
Authors: McDonald Kathryn M. 
Published: January 2014

AbstractFor diagnoses that require inputs from multiple players – patients, informal caregivers, health care professionals – a team approach to coordinating diagnostic care has the potential to improve outcomes. Taking a patient-system perspective helps elucidate important factors relevant to team-based diagnostic performance. These factors are conceptualized as a metaphoric playing field that has goals, players interacting, unavoidable uncertainties and influential social conditions specifically tied to the diagnosis phase of care. In terms of rules of the game, the patient-system interaction might be guided by application of Gittell and colleague’s relational coordination concepts, and understood within a broader social network framework of diagnosis, adapted from work by Berkman and colleagues. Patient-centered metrics are also needed to monitor the success of diagnosis.
A Compact Human-Powered Energy Harvesting System
Authors: Rao Yuan , McEachern Kelly M. , Arnold David P. 
Published: January 2014

Abstract
A fully functional, self-sufficient body-worn energy harvesting system is presented in this paper. The system is designed for passively capturing energy from human motion, with the long-term vision of supplying power to portable, wearable, or even implanted electronic devices. Compared with state-of-the-art vibrational systems, the system requires no external power supplies and can bootstrap from zero-state-of-charge to generate electrical energy from walking, jogging, and cycling; convert the induced AC voltage to DC voltage; and then boost and regulate the DC voltage to charge a Li-ion-polymer battery. Measurements show that at open-load the system turns on when the input is above 1 Vpk and turns off when the input drops below about 600 mVpk with no measurable standby power consumption. Tested under normal human activities (walking, jogging, and cycling) when worn on different parts oze body, the 70 cm3 system is shown to charge a 3.7 V rechargeable battery at charge rates ranging from 33 μW to 234 μW.
Porous functional poly(unsaturated polyester-co-glycidyl methacrylate-co-divinylbenzene) polyHIPE beads through w/o/w multiple emulsions: preparation, characterization and application
Authors: Mert Emine Hilal , Yıldırım Hüseyin 
Published: January 2014

AbstractPoly(unsaturated polyester-co-glycidyl methacrylate-co-divinylbenzene) poly high internal phase emulsion (HIPE) beads were synthesized via water-in-oil-in-water (w/o/w) multiple emulsions. HIPEs were prepared by using a commercial unsaturated polyester resin (UPR) and a mixture of glycidyl methacrylate (GMA) and divinylbenzene (DVB) as the cross-linker. The external surfactant was found to be a strong influence on the morphology of the beads. The porosity and the pore morphology of the resulting polyHIPE beads were investigated by scanning electron microscopy (SEM) and Brunauer-Emmett-Teller (BET) molecular adsorption method, respectively. Post-functionalization of the beads was carried out with multifunctional amines such as 1,4-ethylenediamine (EDA), 1,6-hexamethylenediamine (HMDA) and 4-aminosalicylic acid (ASA). Elemental analysis was used to confirm the functionalization. Resulting functional beads were tested on the adsorption of Ag(I), Cu(II), and Cr(III) under non-competitive conditions and atomic absorption spectroscopy (AAS) was used to calculate the adsorption capacities. The maximum adsorption capacities of the functional beads were found to be decreasing in the order of Ag(I)>Cu(II)>Cr(III).
Provision of Public Goods and Violent Conflict: Evidence from Colombia
Authors: Cortés Darwin , Montolio Daniel 
Published: January 2014

AbstractThe Colombian conflict has lasted for around 50 years. It has been fueled by the financial opportunities coming from production and traffic of illegal drugs, and predation of other natural resources. In such a context it is not clear what policies are more effective to reduce conflict. Two public policies that are frequently mentioned as effective to reduce conflict are investments in roads and education. However, a priori, both investments in roads and education may either increase or reduce conflict. After controlling for possible problems of endogeneity, we show that increases in roads provision reduces conflict while education does not. Because this is robust to controlling for measures of state capacity and governance, and the opportunity cost of conflict, our results are likely to be explained by the relative mobility of education and roads. Policies that increase roads provision might help to fight against the intensity of conflict.
Integrative Chemistry toward Biosourced SiC Macrocellular Foams Bearing Unprecented Heat Transport Properties
Authors: Ungureanu Simona , Birot Marc , Vignoles Gérard , Lorette Christophe , Sigaud Gilles , Deleuze Hervé , Backov Rénal 
Published: January 2014

ABSTRACTBlack liquor is a by-product of the paper mill Kraft process that deserves more valorization than its present use as low-grade fuel. In this work, SiC/C composite foams were prepared for the first time from concentrated emulsions by carbothermal reduction of bio-sourced precursors combining sodium silicate by lignin at 1400°C. The composition of the materials was determined by XRD, FTIR and Raman analyses. Their porous structure was characterized by SEM, mercury intrusion porosimetry, and nitrogen sorption, while their thermal properties were measured by TGA and dynamic DSC. Concerning their heat transport properties, we found out that when the starting lignin content was increased, the final C/Si ratio, the specific surface area and the heat diffusivity increased as well. Its high values were attributed to a cooperative effect between radiative heat transfer and the presence of partially graphitized carbon.
Applications of Shaped Femtosecond near-IR Laser Irradiation in the Generation of Metal Nanoparticles
Authors: Tangeysh Behzad , Tibbetts Katharine Moore , Odhner Johanan H. , Wayland Bradford B. , Levis Robert J. 
Published: January 2014

ABSTRACTFemtosecond near IR laser irradiation is explored as a general methodology to produce metal nanoparticles from metal precursor solutions. Initial studies of the formation and transformations of gold nanoparticles in aqueous solution are used as model processes to evaluate the effects of laser parameters, reaction medium and surfactants in controlling metal nanoparticle formation. The addition of polymer surfactants such as poly(ethylene glycol) (PEG) was found to significantly accelerate Au(III) reduction as compared to surfactant-free systems. Photo-reduction for aqueous solutions of Au(III) in the presence of PEG results in relatively small narrowly dispersed spherical gold nanoparticles compared to relatively large well-formed crystalline nanoparticles that are observed in the absence of surfactants. Varying the concentration of PEG is an effective approach to tune the diameter and size distribution from 3.9±0.7 nm to 11±2.4 nm for Au nanoparticles produced by laser processing.
Fabrication of Piezoelectric-Rubber of Large Piezoelectric Property
Authors: Mamada Shogo , Yaguchi Naoyuki , Hansaka Masanori , Yamato Masafumi , Yoshida Hirohisa 
Published: January 2014

ABSTRACTIn the previous investigation, piezoelectric properties of the ‘Aligned-type’ in which the piezoelectric-ceramic particles are formed in linear aggregates in the rubber, remarkable piezoelectric properties were confirmed. In this investigation, to further enhance the piezoelectric properties of the Aligned-type, the influence of the matrix properties was investigated. The properties on which we focused were the dielectric constant and the Young’s modulus. Four kinds of matrix materials whose dielectric constant and Young’s modulus are different from each other; Silicone gel, Silicone rubber, Urethane rubber and Poly-methyl-methacrylate were investigated. As a result of measurement of the piezoelectric strain constant d33 of the Aligned-Type, it was confirmed that though the influence of the dielectric constant of the matrix material was small, the lower the Young’s modulus of the matrix was, the higher d33 was.
Time Interval and Continuous Testing of Stimuli Responsive Hydrogels
Authors: Bates Jeffrey S. , Magda Jules J. 
Published: January 2014

ABSTRACTResearchers have investigated hydrogels as potential materials for biological monitoring. Hydrogel compositions have been designed to respond to changes in temperature, pH, glucose concentration and ionic strength concentration. Hydrogels designed to respond to changes in environmental conditions have demonstrated their ability to respond via a swelling or shrinking action. This swelling behavior can be exploited using a variety of signal transduction methods. While this technology shows promise, the degradation of hydrogel materials has not yet been characterized with respect to the shelf life of hydrogel samples or to their use in continuous testing. A series of experiments were performed to test hydrogels stored for extended periods of time then subjected to oscillatory testing, and the results have been analyzed to determine whether hydrogels can be used for extended periods of time for biological sensing applications.