bluuebunny/crossref_metadata_2025_split huggingface dataset:
Expression of proteins in serum, synovial fluid, synovial membrane, and articular cartilage samples obtained from dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament disease and dogs without stifle joint arthritis
Authors: Garner Bridget C. , Kuroki Keiichi , Stoker Aaron M. , Cook Cristi R. , Cook James L. 
Published: February 2013

Abstract
Objective—To identify proteins with differential expression between healthy dogs and dogs with stifle joint osteoarthritis secondary to cranial cruciate ligament (CCL) disease.
Sample—Serum and synovial fluid samples obtained from dogs with stifle joint osteoarthritis before (n = 10) and after (8) surgery and control dogs without osteoarthritis (9) and archived synovial membrane and articular cartilage samples obtained from dogs with stifle joint osteoarthritis (5) and dogs without arthritis (5).
Procedures—Serum and synovial fluid samples were analyzed via liquid chromatography–tandem mass spectrometry; results were compared against a nonredundant protein database. Expression of complement component 3 in archived tissue samples was determined via immunohistochemical methods.
Results—No proteins had significantly different expression between serum samples of control dogs versus those of dogs with stifle joint osteoarthritis. Eleven proteins (complement component 3 precursor, complement factor I precursor, apolipoprotein B-100 precursor, serum paraoxonase and arylesterase 1, zinc-alpha-2-glycoprotein precursor, serum amyloid A, transthyretin precursor, retinol-binding protein 4 precursor, alpha-2-macroglobulin precursor, angiotensinogen precursor, and fibronectin 1 isoform 1 preproprotein) had significantly different expression (> 2.0-fold) between synovial fluid samples obtained before surgery from dogs with stifle joint osteoarthritis versus those obtained from control dogs. Complement component 3 was strongly expressed in all (5/5) synovial membrane samples of dogs with stifle joint osteoarthritis and weakly expressed in 3 of 5 synovial membrane samples of dogs without stifle joint arthritis.
Conclusions and Clinical Relevance—Findings suggested that the complement system and proteins involved in lipid and cholesterol metabolism may have a role in stifle joint osteoarthritis, CCL disease, or both.
Comparison of efficacy and duration of effect on corneal sensitivity among anesthetic agents following ocular administration in clinically normal horses
Authors: Pucket Jonathan D. , Allbaugh Rachel A. , Rankin Amy J. , Ou Zhining , Bello Nora M. 
Published: February 2013

Abstract
Objective—To compare efficacy and duration of effect on corneal sensitivity of 0.5% proparacaine hydrochloride, 0.5% bupivacaine hydrochloride, 2% lidocaine hydrochloride, and 2% mepivacaine hydrochloride solutions following ocular administration in clinically normal horses.
Animals—68 clinically normal horses.
Procedures—60 horses were assigned to receive 1 anesthetic agent in 1 eye. For each of another 8 horses, 1 eye was treated with each of the anesthetic agents in random order with a 1-week washout period between treatments. Corneal sensitivity was assessed via corneal touch threshold (CTT) measurements obtained with a Cochet-Bonnet aesthesiometer before and at 1 minute, at 5-minute intervals from 5 to 60 minutes, and at 10-minute intervals from 60 to 90 minutes after application of 0.2 mL of anesthetic agent. General linear mixed models were fitted to the CTT data from each of the 2 experimental groups to assess the effects of the anesthetic agents over time, accounting for repeated observations within individual horses.
Results—Corneal sensitivity decreased immediately following topical application of each anesthetic agent; effects persisted for 35 minutes for proparacaine and mepivacaine treatments, 45 minutes for lidocaine treatment, and 60 minutes for bupivacaine treatment. Maximal CTT reduction was achieved following application of bupivacaine or proparacaine solution, whereas mepivacaine solution was least effective.
Conclusions and Clinical Relevance—Ocular application of each evaluated anesthetic agent reduced corneal sensitivity in horses; although 0.5% proparacaine or 2% lidocaine solution appeared to induce adequate short-duration corneal anesthesia, use of 0.5% bupivacaine solution may be more appropriate for procedures requiring longer periods of corneal anesthesia.
Metal Nanoparticles and Plants / Nanocząstki Metaliczne I Rośliny
Authors: Masarovičová Elena , Kráľová Katarína 
Published: February 2013

Abstract
Metal nanoparticles (MNPs) belong mostly to the engineered type of nanoparticles and have not only unique physical and chemical properties but also different biological actions. In recent years, noble MNPs and their nano-sized agglomerates (collectively referred to as nanoparticles or particles in the subsequent sections) have been the subjects of much focused research due to their unique electronic, optical, mechanical, magnetic and chemical properties that can be significantly different from those of bulk materials. To enhance their use, it is important to understand the generation, transport, deposition, and interaction of such particles. Synthesis of MNPs is based on chemical or physical synthetic procedures and by use of biological material (“green synthesis” as an environmentally benign process) including bacteria, algae and vascular plants (mainly metallophytes). In biological methods for preparation of metal nanoparticles mainly leaf reductants occurring in leaf extracts are used. MNPs can be formed also directly in living plants by reduction of the metal ions absorbed as a soluble salt, indicating that plants are a suitable vehicle for production of MNPs. These methods used for preparation of MNPs are aimed to control their size and shape. Moreover, physicochemical properties of MNPs determine their interaction with living organisms. In general, inside the cells nanoparticles might directly provoke either alterations of membranes and other cell structures or activity of protective mechanisms. Indirect effects of MNPs depend on their physical and chemical properties and may include physical restraints, solubilization of toxic nanoparticle compounds or production of reactive oxygen species. Toxic impacts of MNPs on plants is connected with chemical toxicity based on their chemical composition (eg release of toxic metal ions) and with stress or stimuli caused by the surface, size and shape of these nanoparticles. Positive effects of MNPs were observed on the following plant features: seed germination, growth of plant seedlings, stimulation of oxygen evolution rate in chloroplasts, protection of chloroplasts from aging for long-time illumination, increase of the electron transfer and photophosphorylation, biomass accumulation, activity of Rubisco, increase of quantum yield of photosystem II, root elongation, increase of chlorophyll as well as nucleic acid level and increase in the shoot/root ratio. However, it should be stressed that MNPs impact on human and environmental health remains still unclear.
Chemical Denudation in a Geoecosystem in Acid Immision Conditions / Denudacja Chemiczna W Geoekosystemie W Warunkach Kwaśnej Imisji
Authors: Kozłowski Rafał , Jóźwiak Marek 
Published: February 2013

Abstract
There is an analysis of the physico-chemical properties and the chemical composition of precipitation in a forest geoecosystem in acid immision conditions. The data used in the analysis was collected between 2000 and 2010. In that period, a drop in the immision scale of S-SO2 in the atmospheric air had been observed, which consequently was one of the reasons for a drop in the precipitation acidity. The conclusion was that rainwater, after penetrating treetops and trunks, undergoes a significant transformation whose intensity depends on species composition of the forest stand. Statistically relevant differences were found in coniferous stands, in which a substantial drop in pH was noted, in addition to an increase in the load of components accumulated in the soil in relation to atmospheric precipitation. The calculated indicators of the eco-chemical state of waters (Ma%) have revealed that throughfall waters in coniferous stands and fir and beech stemflow contain significant amounts of aluminium, manganese, iron and hydrogen ions in the sum of cations. Also the indicator of acid-neutralizing capacity (ANCaq) shows that these waters are more enriched with sulfate, nitrate and chloride ions than bulk precipitation. The recorded variation eventuates from processes related to dry and humid deposition, as well as ion-exchange processes occurring within the stand. The obtained results point to an intensification of chemical denudation within the trunks, triggering disadvantageous changes in both biotic and abiotic part of the ecosystem. The effects of the increased inflow of souring agents to the soil through stemflow are significant acidification of the top mineral soil horizon and an increase in the hydrolytic acidity.
Water Effect on Oxidative Desulphurization Process of Straight-Run Kerosene Fraction / Wpływ Wody Na Utleniający Proces Odsiarczania Frakcji Nafty
Authors: Pyshyev Serhiy , Bratychak Michael , Hayvanovych Vasyl , Paniv Pavlo , Wacławek Witold 
Published: February 2013

Abstract
Sulphur dioxide obtained during fuels burning in combustion engines is one of the main pollutants. In diesel oils and gasolines the sulphur content must be 5-10 ppm and in jet fuels - 300-3000 ppm. However the production of hydrofined jet fuel is problematic. The reason is deterioration of fuel stability and antioxygenic properties. The oxidative desulphurization of straight-run kerosene was investigated. This method combines oxidation by atmospheric oxygen of sulphur compounds under increased temperature and pressure in the presence of water in the reaction medium, and removal of oxidized sulphur compounds from the oxidation-treated fuel via rectification. It was showed that water partially extracts from the hydrocarbon medium acidic compounds, formed in the beginning stage of oxidation, dissociation of which leads to the formation in water acidic medium. As a result, a pathway of the hydroperoxides decomposition partially changes from the formation of carbonic acids and oxyacids to the formation of alcohols, phenols and alkylphenols, which displayed an inhibitory effect in hydrocarbon oxidation. It was assumed that an inhibitory effect of water, in addition to the creation reverse micelles with peroxides and complexes with free radicals, caused by oxidation products created in the beginning stage of oxidation. The effect of water/kerosene ratio on the oxidative desulphurization of straight-run kerosene fraction has been examined. It was found that water improves process selectivity with insignificant influence on the degree of sulphur recovery. The optimum value of water/kerosene ratio for the fuel containing 0.15% mass of sulphuric compounds has been determined.
Pyrolysis Process of Whole Waste Tires as A Biomass Energy Recycling / Piroliza Opon Samochodowych Jako Energetyczny Recykling Biomasy
Authors: Ryms Michał , Januszewicz Katarzyna , Lewandowski Witold M. , Klugmann-Radziemska Ewa 
Published: February 2013

Abstract
The article is devoted to the description of material recycling of whole waste tires, including a new method of pyrolysis process, resulting in the final products: technically oil fractions (rubber plasticizer of rubber compounds) or diesel fuels (light, medium and heavy), activated carbon, gas fuel and steel scrap. Operational and performance tests of the first version of the pilot plant, consisting of three contributions cooperating with one pyrolytic reactor confirmed that this technology is applicable but has still some flaws and errors, both structural and technological. Usually such errors in the first test of technology development cannot be avoided. This paper describes: pyrolysis processes which occurs in the pilot plant, protected by a patent application design of continuously working prototype installation for recycling of tire and identifies future directions of research in this field
Utilization of Ends From Pvc Production With Application of Fe-Cr Catalyst - Dioxins Hazard / Utylizacja Odpadu Z Produkcji Pcw Z Udziałem Katalizatora Fe-Cr W Aspekcie Zagrożenia Dioksynami
Authors: Żarczyński Andrzej , Zaborowski Marcin , Gorzka Zbigniew , Kaźmierczak Marek 
Published: February 2013

Abstract
The subject of the investigation was the model solution containing 50 g/dm³ waste light ends from PVC production, dissolved in the ethanol-water (1:1) mixture. The main components of light ends were as follows: trichloromethane, tetrachloromethane, and chloroderivatives of ethane. Granular ferric-chromic catalyst (TZC 3/1) was used in the investigation. The temperature range applied in experiments was 400-600ºC and the contact time was 0.27 s. Gaseous products of the reaction were analysed in order to determine among others concentration of chlorine, formaldehyde, oxygen, carbon monoxide and dioxins. The content of total organic carbon (TOC), chloride ions and formaldehyde was determined in a condensate. Oxidation of the mixture proceeded in the all temperature range with high efficiency in regard to initial TOC value of the solution. The concentration of dioxins in the combustion gases obtained in the process carried out in temperature 450ºC amounted to 0.021-0.027 ng TEQ/m3, and was significantly lower than the admissible value of 0.1 ng TEQ/m3. Congeners of polychlorinated dibenzofuranes (PCDFs) predominated in the combustion gases. Tested catalyst did not undergo deactivation during 150 h substrate oxidation.
Community Composition and Abundance of Protozoa Under Different Concentration Of Nitrogen Compounds at “Hajdow” Wastewater Treatment Plant / Zbiorowiska Pierwotniaków I Ich Liczebność W Oczyszczalni Ścieków „Hajdów” Przy Różnych Stężeniach Związków Azotu
Authors: Jaromin-Gleń Katarzyna , Babko Roman , Łagód Grzegorz , Sobczuk Henryk 
Published: February 2013

Abstract
The protists notably ciliates and testate amoebas in the conditions of modernized wastewater treatment plants “Hajdow” (Lublin, Poland) and the nitrogen removal efficiency were studied. Sampling took place every week during the period of August-September 2010. The total of 31 taxa of ciliates and 3 taxa of testate amoebas were identified. Most of the species were registered during the whole research period. Significant abundance reached the following species: Acineria uncinata, Aspidisca cicada, Aspidisca lynceus, Vorticella aquadulcis, Vorticella infusionum, Arcella vulgaris, Euglypha acanthophora and Pyxidicula operculata. In September increase in efficiency of nitrogen removal process was registered. The research shows that the increase of nitrogen reduction efficiency was accompanied by increase of ciliates quantity, mainly due to peritrichid ciliates.
Heavy Metals Baseline Concentrations in Soft Tissues of Patella Sp. From the Stratoni Coastal Environment, Ne Greece / Bazowy Poziom Zanieczyszczeń Metalami Ciężkimi W Tkankach Miękkich Patella Sp. Występujących W Przybrzeżnych Obszarach Stratoni, Grecja
Authors: Kelepertzis Efstratios 
Published: February 2013

Abstract
Species of the limpet Patella sp. were collected from a typical control coastal site (Artemida) and a metal contaminated marine environment (Stratoni), situated in Greece. The soft tissues were analyzed for their heavy metal (Pb, Zn, Mn, Cu, Ni, Cr) content by flame atomic absorption spectroscopy. High Pb, Zn and Cu concentrations were determined in limpet samples from Stratoni, which can be attributed to geological-mineralogical factors due to the natural heavy metal elevated substrate of the broader mineralized area. In contrast, concentrations of Mn, Ni and Cr in both areas are comparable to those reported for other non-polluted geographical regions of the Mediterranean area. Considering the refurbishment of the Stratoni port that will accompany the new large scale mining operations, these results provide a preliminary baseline metal level in these organisms, by which future local changes could be assessed. It is suggested that the species of Pattella sp. are appropriate to be used for biomonitoring research and should be included in routine monitoring programs regarding the Stratoni marine ecosystem. Further studies of metal levels in limpets of Patella sp. from the Stratoni area including the investigation of the possible effects of seasonal changes on metal concentrations and distribution are required.
Assessment of Airborne Actinomycetes in Subterranean and Earth Sanatoriums / Występowanie Promieniowców W Pomieszczeniu Sanatoriów Podziemnym Oraz Naziemnym
Authors: Frączek Krzysztof , Kozdrój Jacek 
Published: February 2013

Abstract
Actinomycetes are common soil microorganisms; however, they have also been detected in air samples, becoming important air contaminants in occupational and residential habitats. Concentrations of airborne actinomycetes and their distribution among different fractions of bioaerosol were determined in selected sites of two sanatorium types, ie a subterranean located in the former salt-mine of Bochnia and standard earth spa of Szczawnica. The average concentrations of actinomycetes ranged from 0 to almost 40 cfu m-3, for the sanatorium of Bochnia, and from 7 to 125 cfu m-3, for Szczawnica spa, throughout the year. The respiratory fraction of the bioaerosol ranged from 25 to 51% and from 78 to 81% in the subterranean sites and rooms of Szczawnica spa, respectively. For both sanatoriums, among isolated actinomycetes dominated species belonging to Rhodococcus, Streptomyces and Nocardia. The results indicate that patients can be differentially exposed to airborne actinomycetes in compared sanatoriums. The air of underground spa reveals to be of better quality, regarding the bacteria characteristics.
Analysis of Solar Energy Resources in Southern Poland for Photovoltaic Applications / Analiza Struktury Zasobów Energii Słonecznej Obszaru Polski Południowej Do Zastosowań Fotowoltaicznych
Authors: Rodziewicz Tadeusz , Teneta Janusz , Zaremba Aleksander , Wacławek Maria 
Published: February 2013

Abstract
The article presents an analysis of the resources and the structure of the solar energy in the area of Southern Poland on the basis of complete meteorological data from the AGH University of Science and Technology in Krakow in 2009. An analysis attempt of its use for photovoltaic conversion using different modules with different spectral characteristics of absorbers was made. These latest methods for characterizing the structure of solar energy resources such as: distributions throughout the year: sky clearness or cloudiness indexes, the average values of photon energy (APE) and the contents of the useful fraction (UF) of the solar spectrum, are not yet widely known and used as in Poland and in other EU countries, despite the fact that most accurately determine the spectral matching factor for the chosen photovoltaic module. Due to the need for a very expensive measuring equipment, are used only by a few laboratories in the European Union, such as CREST (Centre for Renewable Energy Systems Technology) in the UK. The article presents - developed and used in the Opole University - a new low-cost method for determining of the spectrum with the use of above-mentioned indexes, including APE and UF, without buying an expensive spectroradiometer, which gives comparable results.
Initiating Biodegradation of Polyvinylpyrrolidone in an Aqueous Aerobic Environment: Technical Note / Zainicjowanie Biodegradacji Poliwinylopirolidonu W Środowisku Wodno-Tlenowym: Notatki Techniczne
Authors: Julinova Marketa , Kupec Jan , Slavik Roman , Vaskova Maria 
Published: February 2013

Abstract
A synthetic polymer, polyvinylpyrrolidone (PVP - E 1201) primarily finds applications in the pharmaceutical and food industries due to its resistance and zero toxicity to organisms. After ingestion, the substance passes through the organism unchanged. Consequently, it enters the systems of municipal wastewater treatment plants (WWTP) without decomposing biologically during the waste treatment process, nor does it attach (through sorption) to particles of activated sludge to any significant extent, therefore, it passes through the system of a WWTP, which may cause the substance to accumulate in the natural environment. For this reason the paper investigates the potential to initiate aerobic biodegradation of PVP in the presence of activated sludge from a municipal wastewater treatment plant. The following agents were selected as the initiators of the biodegradation process - co-substrates: acrylamide, N-acethylphenylalanine and 1-methyl-2-pyrrolidone, a substance with a similar structure to PVP monomer. The biodegradability of PVP in the presence of co-substrates was evaluated on the basis of biological oxygen demand (BOD) as determined via a MicroOxymax O2/CO2/CH4 respirometer. The total substrate concentration in the suspension equaled 400 mg·dm-3, with the ratio between PVP and the cosubstrate being 1:1, while the concentration of the dry activated sludge was 500 mg·dm-3. Even though there was no occurrence of a significant increase in the biodegradation of PVP alone in the presence of a co-substrate, acrylamide appeared to be the most effective type of co-substrate. Nevertheless, a recorded decrease in the slope of biodegradation curves over time may indicate that a process of primary decomposition was underway, which involves the production of metabolites that inhibit activated sludge microorganisms. The resulting products are not identified at this stage of experimentation.
Molecular Detection of Prostate Cancer by Methylation-Specific Polymerase Chain Reaction from Urine Specimens
Authors: Dumache Raluca , Popescu Sorina , Minciu Radu , Negru Serban , Puiu Maria 
Published: February 2013

Summary
Background: Prostate cancer (PCa) represents the second most prevalent malignancy among males, which is characterized by a high mortality rate. The aim of our study was to evaluate the methylation status of glutathione S-transferase P1 (GSTP1) in urine specimens from males with PCa and benign prostatic hyperplasia (BPH) and its usefulness in distinguishing between males with PCa and BPH by noninvasive methods.
Methods: Voided urine specimens were collected from 65 patients with PCa and 45 patients with BPH. Genomic DNA was isolated and subjected to bisulfite modification. Methylation status of the GSTP1 gene was determined by conventional methylation-specific polymerase chain reaction (MSP) analysis.
Results: Promoter hypermethylation of the GSTP1 gene in voided urine samples was found in 63 of 65 (97%) males with PCa and in 5 of 45 (11%) males with BPH. The sensitivity and specificity of GSTP1 in discriminating between PCa and BPH males were 98% and 89%, respectively.
Conclusions: Gene analysis of GSTP1 using conventional MSP in urine specimens can be used as a noninvasive biomarker to distinguish between men with malignant and benign prostatic diseases.