bluuebunny/crossref_metadata_2025_split huggingface dataset:
How to explain exercise-induced phenotype from molecular data: rethink and reconstruction based on AMPK and mTOR signaling
Authors: Qi Zhengtang , Zhai Xiaofeng , Ding Shuzhe 
Published: December 2013

Abstract
During endurance and resistance exercise training, AMPK and mTOR signaling were known as selective pathways implicating the differentiation of exercise-induced phenotype in skeletal muscle. Among the previous studies, however, the differences in exercise protocol, the individuality and the genetic heterogeneity within species make it difficult to reach a consistent conclusion in the roles of AMPK and mTOR signaling. In this review, we aim not to reanalyze the previous articles and present the research progress of AMPK and mTOR signaling in exercise, but to propose an abstract general hypothesis for exercise-induced phenotype. Generally, exercise- induced skeletal muscle phenotype is independent of one and a few genes, proteins and signaling pathways. Convergent adaptation will better summarize the specificity of skeletal muscle phenotype in response to a single mode of exercise. Backward adaptation will open a new concept to illustrate the process of exercise-induced adaptation, such as mitochondrial quality control and muscle mass homeostasis.
Stability and prognostic value of Slug, Sox9 and Sox10 expression in breast cancers treated with neoadjuvant chemotherapy
Authors: Riemenschnitter Cosima , Teleki Ivett , Tischler Verena , Guo Wenjun , Varga Zsuzsanna 
Published: December 2013

Abstract

Background
Expression of transcription-factors as Slug and Sox9 was recently described to determine mammary stem-cell state. Sox10 was previously shown to be present also in breast cancer. Protein overexpression of Slug, Sox9 and Sox10 were associated with poor overall survival and with triple-negative phenotype in breast cancer. In this study we tested the stability of Slug, Sox9 and Sox10 expression during chemotherapy and addressed their prognostic role of in neoadjuvant treated primary breast-cancer and their correlation to pathological-response and overall survival.

Methods
We analyzed immunohistochemical expression of Slug, Sox9 and Sox10 in tissue microarrays of 96 breast cancers prior to and after neoadjuvant chemotherapy. Expression was evaluated in invasive tumor cells and in tumor stroma and scored as 0, 1+, 2+ 3+. Expression-profile prior to and after chemotherapy was correlated to overall survival (Kaplan Meier) and with established clinico-pathological parameter.

Results
Sox9, Sox10 and Slug were expressed in 82–96% of the tumor cells prior to chemotherapy. Slug was expressed in 97% of the cases in tumor stroma before therapy. Change in expression-profile after chemotherapy occurred only in Slug expression in tumor-cells (decreased from 82 to 51%, p = 0.0001, Fisher’s exact test). The other markers showed no significant change after chemotherapy. Stromal Sox9 expression (0 to 2+) correlated to better overall survival after chemotherapy (p = 0.004) and reached almost statistical significance prior to chemotherapy (p = 0.065). There was no correlation between Sox9 and hormone-receptor expression. In multivariate-analysis, the stromal Sox9 expression after chemotherapy proved to be an independent and better prognostic marker than hormone-receptor status. Other clinico-pathological parameter (as HER2-status or pathological-stage) showed no correlation to the analyzed markers.

Conclusion
Strong stromal Sox9 expression in breast cancer after chemotherapy was found to bear negative prognostic information and was associated with shortened overall survival. Slug expression was significantly changed (reduced) in samples after neoadjuvant chemotherapy.
Histone deacetylase isoforms regulate innate immune responses by deacetylating mitogen-activated protein kinase phosphatase-1
Authors: Jeong Youngtae , Du Ronghui , Zhu Xiaolei , Yin Shasha , Wang Jian , Cui Hengmi , Cao Wangsen , Lowenstein Charles J 
Published: December 2013

AbstractThe MAPK pathway mediates TLR signaling during innate immune responses. We discovered previously that MKP-1 is acetylated, enhancing its interaction with its MAPK substrates and deactivating TLR signaling. As HDACs modulate inflammation by deacetylating histone and nonhistone proteins, we hypothesized that HDACs may regulate LPS-induced inflammation by deacetylating MKP-1. We found that mouse macrophages expressed a subset of HDAC isoforms (HDAC1, HDAC2, and HDAC3), which all interacted with MKP-1. Genetic silencing or pharmacologic inhibition of HDAC1, −2, and −3 increased MKP-1 acetylation in cells. Furthermore, knockdown or pharmacologic inhibition of HDAC1, −2, and −3 decreased LPS-induced phosphorylation of the MAPK member p38. Also, pharmacologic inhibition of HDAC did not decrease MAPK signaling in MKP-1 null cells. Finally, inhibition of HDAC1, −2, and −3 decreased LPS-induced expression of TNF-α, IL-1β, iNOS (NOS2), and nitrite synthesis. Taken together, our results show that HDAC1, −2, and −3 deacetylate MKP-1 and that this post-translational modification increases MAPK signaling and innate immune signaling. Thus, HDAC1, −2, and −3 isoforms are potential therapeutic targets in inflammatory diseases.
Language Self-Identification of Informal Communication Sphere
Authors: Ларионова  , Larionova Alla 
Published: December 2013

The paper is devoted to informal communication as a specific part of modern communicative space. This part significantly extends subjective-author’s sphere and local and quantitative representation in different areas of human activities. Informal communication is observed as a system that
possesses language self-identification determined by linguo-creative thinking respective to language and reality.
Indirect Speech Acts in the Russians’ Everyday Communication
Authors: Нестерова  , Nesterova T. 
Published: December 2013

The phenomena of indirect communication (namely contextual-situational

indirect speech acts) which are typical for the situations of the Russians’

everyday communication: situations of addressing, attention attracting;

acquaintance; greeting; farewell; congratulation; wish; gratitude; apology;

request; consent, permission; disagreement, objection, refusal; invitation;

offer; council; consolation, sympathy, condolence; compliment, approval,

praise have been described in this paper. Direct and conventional indirect

speech acts, realizing the aforementioned intensions, have been specified

from the speech etiquette point of view. The system research of indirect,

contextual-situational oblique speech acts in the given situations of communication has not been practically carried out.

Considering the cooperativity zone (zone of communicators’ benevolent relations), the author has picked out and described the main types of contextual-situational indirect speech acts (including hints and manipulation; syncretic and transposed forms), demonstrated their combinatory peculiarities

in the structure of indirect speech genres. Creation of typology related to

contextual- situational indirect speech acts (as well as other phenomena of

indirect communication) in cooperative situations of the Russians’ everyday

communication makes a certain contribution to the development of indirect

communication theory and has also a culturological value.

The use of given research results can be valuable both for further study of

everyday converse, cross-cultural communication, speech genres, speech

etiquette and in practical activities of teachers who are taking up the problems of communication and language teaching (including Russian as a foreign language). The ability related to indirect realization of communicative

meanings, their operation and correct decoding promotes effective com-
munication and is necessary for comprehension and interpretation of texts

from fiction and publicistic literature, as well as for translation activities.
DEFINITION OF LEVEL OF ABSTRACTNESS, COMPLEXITY AND INFORMATIONAL CONTENT OF VARIOUS SUBJECTS SCHOOL TEXTBOOK OF PHYSICS
Authors: �����  , Mayer Robert 
Published: December 2013

The technique and results of the content analysis of school textbooks
of physics for 10 and the 11th class, carried out for the purpose of an assessment
of speed of receipt of educational information, and also complexity
and abstractness of various subjects is described. The used method consists in
calculation of number of elements of the training material containing empirical
and theoretical knowledge, formulas and drawings. The following tasks are
solved: 1) the technique of an assessment of number of empirical and theoretical
information in the educational text is developed; 2) in various subjects of
textbooks frequencies of the appeal to theoretical and empirical knowledge,
and also frequencies of use of formulas are determined; 3) speeds of receipt
of empirical and theoretical information are estimated when studying various
subjects of a course of physics; 4) information complexity of a statement of
a training material in various subjects of textbooks is defined; 5) the content
analysis of textbooks taking into account levels of abstractness of empirical
and theoretical knowledge is carried out. Results of the content analysis are
presented on 9 histograms.
CONTRADICTIONS OF SUBJECTS GREENING IN GENERAL EDUCATION
Authors: �����������  , Dzyatkovskaya Elena 
Published: December 2013

Didactic problems which face researchers of education for sustainable
development (ESD) are described. Identification of these problems and
the analysis of trends of their decision is one of deposits of Russian academy of
science in results of Decade of the ESD. In article the characteristic of subjects
greening phenomenon as main form of realization of ESD at comprehensive
school is given. Evolution of approaches to greening of school subjects in Russia
is considered for the last twenty years, in connection with essential changes
in the contents and structure of ecological education. We differentiated rather
independent directions of ecological education at comprehensive school
which were created at different times, but today coexist and supplement
each other: nature protection, classical (scientific), conceptual (in interests of
ESD) ecological education. We prove that formation of ecological education
for SD aggravates a number of former problems of greening and generates
the new. According to the experts of UNESCO, these problems excite also our
foreign colleagues. The conclusion is drawn: external power impacts on subjects
if they contradict internal tendencies of their development, can lead to
ESD imitation, its substitution by education about (not for) SD or to irreparable
changes of systemacity, structure, logic of the subject contents. Trends of development
of the ESD technology are allocated.
Registro de Pachymerus nucleorum (Fabricius) (Coleoptera, Chrysomelidae, Bruchinae) predando sementes de Licuri em Caetés, Pernambuco, Brasil
Authors: Andrade Marcelo Bezerra , Esteves Filho Alberto Belo , Siqueira Izabelle Taynã Dourado de , Giorgi José Adriano 
Published: December 2013

O objetivo deste trabalho foi registrar a predação de sementes de Syagrus coronata (Martius) (Arecaceae) por larvas de Pachymerus nucleorum (Fabricius), no município de Caetés, Pernambuco nordeste do Brasil. Os frutos foram coletadas de 10 plantas adultas de Syagrus coronata (Arecaceae) no entorno da usina experimental de biodiesel de Caetés-PE, após a definição de um raio de 1,5m ao redor da copa, esta foi dividida em quatro quadrantes, e através de sorteio, foi colocado um coletor no quadrante vencedor a 1m da estipe. Na análise os frutos com orifício de saída e/ou pela presença da larva no processo de liberação da amêndoa, o nível de infestação alcançou 80% dos frutos analisados.Em outra situação amêndoas aparentemente sadias, ou seja, sem danos superficiais foram observadas diariamente por 60 dias as quais, ocorreram 18% de emergência de larvas até os 41dias, atingindo o pico da predação entre o décimo e o décimo oitavo dia, com 48% de toda predação.
Record of Pachymerus nucleorum (Fabricius) (Coleoptera, Chrysomelidae, Bruchinae) preying seeds of Licuri in Caetés, Pernambuco, Brazil.
Abstract. The aim of the present study was to observe seed predation by larvae of Pachymerus nucleorum (Fabricius) on seeds of Syagrus coronata (Martius) (Arecaceae) in Caetés, Pernambuco, northeastern Brazil. Fruits of 10 adult plants of S. coronata were collected in the surroundings of the experimental biodiesel plant of Caetés. The projection of the crown within 1,5m from the stipe was devided into four quadrants, one of which was randomly selected for sampling. In the first analysis of the larvae presence or their holes of emergence, the infestation level reached 80% of the analyzed fruits. In an another situation, apparently uninfested fruits (without superficial damage) were daily observed for 60 days, occurring 18% of larvae emergence within 41 days, with the peak of predation between the tenth and the eighteenth day, period in which 48% of total predation was observed.
Ocorrência da Cochonilha Negra Saissetia oleae (Olivier) (Hemiptera: Coccidae) em Oliveiras Olea europaea L. (Oleaceae) cv. Ascolano em Diamantina, Minas Gerais, Brasil
Authors: Soares Marcus Alvarenga , Da Cruz Maria do Céu Monteiro , Martins Larissa Madureira , De Carvalho Raoni Pereira , Pires Evaldo Martins , Mendes de Sá Veríssimo Gibran 
Published: December 2013

Plantas de oliveira Olea europaea L. (Oleaceae) foram introduzidas no município de Diamantina, Minas Gerais, Brasil devido ao potencial local para produzir azeitonas e azeite, fruto e processado com mercado mundial em expansão. O objetivo deste trabalho foi registrar, pela primeira vez, a presença da cochonilha negra, Saissetia oleae (Olivier) (Hemiptera: Coccidae), em oliveiras (cv. Ascolano) em Diamantina. O presente registro mostra que S. oleae precisa ser monitorada, quando da implantação de viveiros de mudas e áreas de cultivo nesta região, por ter potencial para causar danos severos nas plantas e perdas na produção.
Occurrence of Black Scale Saissetia oleae (Hemiptera: Coccidae) in Olive Olea europaea (Oleaceae) cv. Ascolano in Diamantina, Minas Gerais State, Brazil.
Abstract. Olive plants Olea europaea L. (Oleaceae) were introduced in the Diamantina region, Minas Gerais State, Brazil mainly because it is a potential place to produce olives and olive oil, with growing global market. The aim of this study was to record the presence of black scale Saissetia oleae (Olivier) (Hemiptera: Coccidae) on olive (cv. Ascolano) in Diamantina. This record shows that S. oleae needs to be monitored when deploying nurseries and crop areas for its potential to cause severe damage in plants and production losses.
First Records of Mantodea (Insecta, Dictyoptera) from the Serra das Confusões National Park, Piauí State, Brazil
Authors: Menezes Eliomar Cruz , Silva Neto Alberto Moreira , Bravo Quijano Freddy Ruben 
Published: December 2013

We present the first inventory of species of the order Mantodea in the Serra das Confusões National Park, in the semiarid state of Piauí, Brazil. The specimens were collected on 11 consecutive days using light traps and active collecting. Two hundred specimens were collected, distributed among five families, 15 genera, and 17 species (of which nine were identified). These results are all first records for the Serra das Confusões National Park, 14 new records for Caatinga vegetation areas, and one new record for Brazil.
Primeiros Registros de Mantodea (Insecta, Dictyoptera) para o Parque Nacional da Serra das Confusões, Piauí, Brasil
Resumo. Neste trabalho são apresentados os resultados do primeiro inventário para espécies da ordem Mantodea no Parque Nacional da Serra das Confusões, uma unidade de conservação inserida no semiárido brasileiro no estado do Piauí. Os espécimes foram coletados durante 11 dias consecutivos utilizando-se armadilha luminosa e coleta ativa. Foram capturados 200 espécimes distribuídos em cinco famílias, 15 gêneros e 17 espécies, das quais nove foram identificadas. Além dos registros inéditos de Mantodea para o Parque Nacional da Serra das Confusões, estes são também inéditos para o estado do Piauí, assim como 14 novos registros para a Caatinga e um novo registro para o Brasil.
What is the Ideal Weather for Social Wasp Polistes versicolor (Olivier) go to Forage?
Authors: Elisei Thiago , Nunes Juliana , Ribeiro Junior Cleber , Fernandes Junior Aluisio , Prezoto Fabio 
Published: December 2013

The objective of this work was to describe the pattern forage of Polistes versicolor (Olivier) waps, verifying the influence of environmental variables on foraging activity. From January to December of 2007, 24 observations of P. versicolor foraging activity in a eucalypt plantation were accomplished. During 10 hours, the intensity of light, wind speed, air humidity and temperature were registered close to the colony. The P. versicolor foraging activity began at 7:30 a.m. and extended to 6:00 p.m. The average departure of workers per hour was 9.62 ±16.67 (0-84), and arrivals per hour were 9.76 ±18.14 (0-87). The most intense activities of the colonies occurred in the period between 10:00 a.m. and 3:00 p.m. The Spearman´s correlation test revealed that the workers’ departures of P. versicolor were stimulated by the increase of light intensity (p< 0.0001) and air temperature p< 0.0001) and the decrease of the humidity (p< 0.0004). The wind speed, however, did not present representative index correlation with the daily activity of the species (p= 0.54). The multivariate linear regression analysis showed that air temperature is the most influential on the P. versicolor foraging activity (p< 0.0001).This study demonstrated that the best weather for P. versicolor departure to forage is a sunshine day, hot temperature and low humidity. In tropical areas these days are common in the summer.
Qual o Clima Ideal para Vespa Social Polistes versicolor (Olivier) Sair para Forragear?
Resumo. O objetivo deste trabalho foi descrever o padrão de forrageio de Polistes versicolor (Olivier), analisando a influência das variáveis ambientais no forrageio diário. Entre janeiro a dezembro de 2007, foram realizadas 24 observações de colônias de P. versicolor no município de Juiz de Fora, MG, totalizando 240 horas. Durante 10 horas foram registradas, a cada 30 minutos, a intensidade de luz, velocidade do vento, umidade do ar e a temperatura nas proximidades da colônia. A atividade forrageadora de P. versicolor começou às 7h e se estendeu até às 18h. A média de saída para o forrageio por hora foi de 9,62 ± 16,67 (0-84), e a chegada/hora foi de 9,76± 18,14 (0-87). A atividade foi mais intensa no período compreendido entre 10 e 15h. O teste de correlação de Spearman revelou que as trabalhadoras de P. versicolor foram estimuladas a sair pelo aumento da intensidade da luz (p <0,0001), da temperatura do ar (p < 0,0001) e a diminuição da umidade (p <0,0004). Já a velocidade do vento não apresentou índice significativo com a atividade diária da espécie (p = 0,54). A análise de regressão linear múltipla mostrou que a temperatura do ar é a mais influente sobre a atividade forrageadora de P. versicolor (p < 0,0001). O presente estudo revelou que o melhor clima para as operárias de P. versicolor saírem para forragear é com forte intensidade de luz do sol, temperatura elevada e baixa umidade.
Large-Scale Mapping of Transposable Element Insertion Sites Using Digital Encoding of Sample Identity
Authors: Gohl Daryl M , Freifeld Limor , Silies Marion , Hwa Jennifer J , Horowitz Mark , Clandinin Thomas R 
Published: December 2013

Abstract
Determining the genomic locations of transposable elements is a common experimental goal. When mapping large collections of transposon insertions, individualized amplification and sequencing is both time consuming and costly. We describe an approach in which large numbers of insertion lines can be simultaneously mapped in a single DNA sequencing reaction by using digital error-correcting codes to encode line identity in a unique set of barcoded pools.
Genetic Control of Specificity to Steroid-Triggered Responses in Drosophila
Authors: Ihry Robert J , Bashirullah Arash 
Published: December 2013

Abstract
Steroid hormones trigger a wide variety of biological responses through stage- and tissue-specific activation of target gene expression. The mechanisms that provide specificity to systemically released pulses of steroids, however, remain poorly understood. We previously completed a forward genetic screen for mutations that disrupt the destruction of larval salivary glands during metamorphosis in Drosophila melanogaster, a process triggered by the steroid hormone 20-hydroxyecdysone (ecdysone). Here, we characterize 10 complementation groups mapped to genes from this screen. Most of these mutations disrupt the ecdysone-induced expression of death activators, thereby failing to initiate tissue destruction. However, other responses to ecdysone, even within salivary glands, occur normally in mutant animals. Many of these newly identified regulators of ecdysone signaling, including brwd3, med12, med24, pak, and psg2, represent novel components of the ecdysone-triggered transcriptional hierarchy. These genes function combinatorially to provide specificity to ecdysone pulses, amplifying the hormonal cue in a stage-, tissue-, and target gene-specific manner. Most of the ecdysone response genes identified in this screen encode homologs of mammalian nuclear receptor coregulators, demonstrating an unexpected degree of functional conservation in the mechanisms that regulate steroid signaling between insects and mammals.
An Evaluation of High-Throughput Approaches to QTL Mapping in Saccharomyces cerevisiae
Authors: Wilkening Stefan , Lin Gen , Fritsch Emilie S , Tekkedil Manu M , Anders Simon , Kuehn Raquel , Nguyen Michelle , Aiyar Raeka S , Proctor Michael , Sakhanenko Nikita A , Galas David J , Gagneur Julien , Deutschbauer Adam , Steinmetz Lars M 
Published: December 2013

Abstract
Dissecting the molecular basis of quantitative traits is a significant challenge and is essential for understanding complex diseases. Even in model organisms, precisely determining causative genes and their interactions has remained elusive, due in part to difficulty in narrowing intervals to single genes and in detecting epistasis or linked quantitative trait loci. These difficulties are exacerbated by limitations in experimental design, such as low numbers of analyzed individuals or of polymorphisms between parental genomes. We address these challenges by applying three independent high-throughput approaches for QTL mapping to map the genetic variants underlying 11 phenotypes in two genetically distant Saccharomyces cerevisiae strains, namely (1) individual analysis of &gt;700 meiotic segregants, (2) bulk segregant analysis, and (3) reciprocal hemizygosity scanning, a new genome-wide method that we developed. We reveal differences in the performance of each approach and, by combining them, identify eight polymorphic genes that affect eight different phenotypes: colony shape, flocculation, growth on two nonfermentable carbon sources, and resistance to two drugs, salt, and high temperature. Our results demonstrate the power of individual segregant analysis to dissect QTL and address the underestimated contribution of interactions between variants. We also reveal confounding factors like mutations and aneuploidy in pooled approaches, providing valuable lessons for future designs of complex trait mapping studies.