bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impaired Glucose Metabolism, Primary Cilium Defects, and Kidney Cystogenesis in Glycogen Storage Disease Type Ia
Authors: Monteillet Laure , Perrot Gwendoline , Evrard Félicie , Miliano Alexane , Silva Marine , Leblond Alicia , Nguyen Clément , Terzi Fabiola , Mithieux Gilles , Rajas Fabienne 
Published: August 2024

Key Points

Metabolism adaptations due to glucose-6 phosphate accumulation in glycogen storage disease type Ia kidneys, toward a Warburg-like metabolism, promoted cell proliferation.Metabolic perturbations directly affected primary cilium structure and cystogenesis in glycogen storage disease type Ia kidneys.

Background
Glycogen storage disease type Ia (GSDIa) is a rare metabolic disorder caused by mutations in the catalytic subunit of glucose-6 phosphatase (G6PC1). This leads to severe hypoglycemia, and most young patients with GSDIa develop CKD. The kidney pathology is characterized by the development of cysts, which typically occur at an advanced stage of CKD.

Methods
To elucidate the molecular mechanisms responsible for cyst formation, we characterized renal metabolism, molecular pathways involved in cell proliferation, and primary cilium integrity using mice in which G6pc1 was specifically deleted in the kidney from an in utero stage.

Results
GSDIa mice exhibited kidney fibrosis, high inflammation, and cyst formation, leading to kidney dysfunction. In addition, the loss of G6PC1 led to the ectopic accumulation of glycogen and lipids in the kidneys and a metabolic shift toward a Warburg-like metabolism. This metabolic adaptation was due to an excess of glucose-6 phosphate, which supports cell proliferation, driven by the mitogen-activated protein kinase/extracellular signal–regulated kinases and protein kinase B/mammalian target of rapamycin pathways. Treatment of GSDIa mice with rapamycin, a target of the mammalian target of rapamycin pathway, reduced cell proliferation and kidney damage. Our results also identified lipocalin 2 as a contributor to renal inflammation and an early biomarker of CKD progression in GSDIa mice. Its inactivation partially prevented kidney lesions in GSDIa. Importantly, primary cilium defects were observed in the kidneys of GSDIa mice.

Conclusions
Metabolic adaptations because of glucose-6 phosphate accumulation in GSDIa renal tubules, toward a Warburg-like metabolism, promoted cell proliferation and cyst formation in a similar manner to that observed in various cystic kidney diseases. This was associated with downregulation of primary cilium gene expression and, consequently, altered cilium morphology.
Saúde mental sobre as águas: concepções de uma equipe de Unidade Básica de Saúde Fluvial (UBSF)
Authors: Schiavi Cristina Elisa Nobre , Meinhart Marluci , Fagundes de Souza Letícia , Ribeiro da Silva Elisangela , Wolski de Oliveira Rafael 
Published: August 2024

Este artigo apresenta os resultados de uma pesquisa realizada junto à equipe de uma Unidade Básica de Saúde Fluvial (UBSF) que atua no estado do Pará, na Amazônia brasileira. O objetivo da pesquisa foi conhecer e analisar as questões de saúde mental identificadas na população ribeirinha a partir das concepções de trabalhadores de uma Unidade Básica de Saúde Fluvial e compreender como se dá o cuidado em saúde mental prestado por esses profissionais a essa população. Trata-se de uma pesquisa de cunho qualitativo com caráter exploratório-descritivo, com dados coletados através de entrevista semiestruturada e analisadas a partir da análise de conteúdo temática. Foram criadas as seguintes categorias: questões de saúde mental da população ribeirinha identificadas pela equipe da UBSF e atuação da equipe no cuidado em saúde mental da população ribeirinha. Foi identificado que as equipes oferecem escuta e acolhimento ao sofrimento psíquico da população e que é necessário investimento em Educação Permanente em Saúde com foco na saúde mental, para qualificar o cuidado e superar a predominância de um modelo pautado em condutas prescritivas, medicalizantes e tecnicistas que, por sua vez, corroboram o fortalecimento do modelo biomédico e da lógica manicomial, pautados na patologização e na medicalização da vida.

Palavras-chave: Saúde Mental; Atenção Primária à Saúde; Território Sociocultural.
Developing medical English writing skills through the use of verb collocations
Authors: Ghembaza Ahmed Hichem , Chebta Hadjéra 
Published: August 2024

Within the medical science context and after the new legislations and government instructions recently issued, the mastery of English language use has become one of the most important learning objectives within the tertiary education context. Yet, when it comes to pedagogic applications within English as a Medium of Instruction (EMI) context; a few questions are raised regarding the very content of the syllabus and relevant methodologies when approaching the teaching of Medical English. The present paper aims to shed light on the teaching of Medical English through the use of verb collocations. The paper displays the different steps and results of action research that targeted 2nd-year medical science students who were involved in medical English training and who sat for different tests within both summative and formative assessment orientations. The results show that the sample exhibits a relatively average level of collocational competence while their vocabulary knowledge tends to be inadequate translation activities. The researchers suggest the use of electronic corpora as a reliable technique that can help enhance students’ collocational competence.
Keywords: Medical English; translation; verb collocations; writing skills.
Bell's Palsy Post COVID-19 Vaccination: An Unwanted Occurence of Coincidence
Authors: Pramanasari Indra , Chrissanty Chrissanty 
Published: August 2024

Highlight:

The COVID-19 vaccination is one of the methods used to combat the COVID-19 pandemic.
Bell's palsy is one of the unwanted side effects of the COVID-19 vaccination.
The benefits and protective impacts of the COVID-19 vaccine exceed the dangers.

ABSTRACT
Introduction: TOne way to fight the COVID-19 pandemic is through vaccination. Indonesia widely uses a number of vaccines. The inactivated virus vaccine (Sinovac-CoronaVac), the recombinant adenovirus vector vaccine ChAdOx1-S/nCoV-19 (AstraZeneca), the mRNA-1273 vaccine (Moderna), and the BNT162b2 mRNA vaccine (Pfizer-BioNTech) are some of these. The COVID-19 vaccination has reported several cases of acute facial nerve paralysis as an adverse event. Case: A 34-year-old female patient complained of drooping lips to the left side and being unable to close the right eyelid three days after receiving the first dose of the COVID-19 vaccine inactivated virus type (CoronaVac). Physical examination revealed obesity, right lagophthalmos, and right peripheral facial nerve paralysis, but no extremity paresis. The patient was clinically diagnosed with Bell's palsy and received corticosteroid therapy. The patient's complaints improved after 2 weeks of treatment. Reports indicate that several types of COVID-19 vaccines have resulted in acute facial nerve paralysis. The mRNA-type COVID-19 vaccine led to the most widely reported cases of Bell’s palsy. The incidence of Bell's palsy after the COVID-19 vaccine is very rare. Conclusion: Overall, the advantages and protective effects of the COVID-19 vaccine outweigh the risks.
Evaluation of the Applications of Using Global Free Digital Elevation Models and GNSS-RTK Data for Agricultural Purposes in Egypt Using Machine Learning
Authors: Abdallah Ashraf , Al-MISTAREHI Bara' , SHTAYAT Amir 
Published: August 2024

Agriculture is a vital component of Egypt&#039;s economy; therefore, using Digital Elevation Models (DEMs) in agricultural planning in Egypt has significant benefits regarding water management, site appropriateness assessment, flood risk mitigation, and infrastructure construction. It is also essential for planners to make more informed decisions, optimize resource allocation, and support sustainable farming practices. This research paper investigates the accuracy of obtaining DEM data from four free global models (STRM30, ALOS30, COP30, and TanDEM-X90). The global DEM data has been compared to an actual GNSS-RTK DEM data surveyed onsite for two agricultural block areas in Aswan, the southern Government of Egypt. The two blocks are a part of a national project. For Block I and II, the RMSE of the Model STRM30 was 2.92 m and 3.59 m, respectively, indicating a poorer solution. Regarding accuracy, the ALOS30 model ranks third, reporting an RMSE of 2.58 m for block II and 3.30 m for block I. COP30 has an RMSE value of 1.06 m for blocks I and II and.91 m overall. TanDEM-X90 is the most accurate model in this investigation; block I provided an RMSE of 0.90 m with an SD of 0.58 m (SD95% = 0.38 m). After removing the anomalies, the model&#039;s stated RMSE for block II was 0.34 m, with an SD value of 0.62 m and 1.03 m. According to the classification using machine learning algorithms, with an accuracy of 84.7% for block I and 85% for block II, TanDEM-X90 is the best solution.
Flipped Learning and Artificial Intelligence
Authors: López-Villanueva David , Santiago Raúl , Palau Ramon 
Published: August 2024

The potential influence of Artificial Intelligence (AI) is obvious in all the elements of teaching and learning processes. Some of the most used methodological approaches of the last decade have been Flipped Classroom and Flipped Learning. This article explores the intersection between Flipped Learning and AI through qualitative research based on interviews with international experts in the field. The results reveal the significant impact of AI on education, highlighting how AI tools are transforming teaching and learning methodologies. Additionally, the evolution of Flipped Learning with the integration of AI is analyzed, showing how this combination enhances personalized learning and improves student engagement. Finally, the role of the teacher in this new educational paradigm is discussed, emphasizing the need for continuous adaptation and the development of new competencies to fully leverage emerging technologies. With this study, we aim to provide an overview of the opportunities and challenges that AI presents in the context of Flipped Learning.
Biologically Active Compounds of Tomato Fruits Affected by Application of Spirulina, Dunaliella and Chlorella Microalgae Extracts
Authors: Augšpole Ingrīda , Sivicka Irina , Kampuss Kaspars , Semjonovs Pāvels , Missa Imants 
Published: August 2024

The present study aimed to investigate the effect of solution of ethanol extractions of different microalgae species: Spirulina sp., Dunaliella sp. and Chlorella sp. on the accumulation of biologically active compounds in tomato fruits. Ethanol solution as well as treatment with drinking water were used as a control. Tomato cultivar ‘Belle’ F1 (Enza Zaden) was grown in 25 L pots, in peat substrate (pHKCl 5.5, producer Laflora LTd), polycarbonate greenhouse. Chemical composition – total carotenoids, lycopene and β-carotene content, total phenolics and anthocyanins, organic acids, vitamin C as well as dry matter, pH and soluble solids content – was determined in tomato fruits at the stage of full ripen. The influence of tested extracts on biologically active compounds on quality parameters of tomatoes was different, but no negative effect was observed. As only pH values as well as total phenol content and total soluble solids had statistically significant differences, results can be described with genetical stability of the cultivar.
Acoustic Transmission Measurements for the Mechanical Properties Extraction of Complex 3D MEMS Transducers
Authors: Becker Dennis , Littwin Moritz , Bittner Achim , Dehé Alfons 
Published: August 2024

Recent publications on acoustic MEMS transducers present a new three-dimensional folded diaphragm that utilizes buried in-plane vibrating structures to increase the active area from a small chip volume. Characterization of the mechanical properties plays a key role in the development of new MEMS transducers, whereby established measurement methods are usually tailored to structures close to the sample surface. In order to access the lateral vibrations, extensive and destructive sample preparation is required. This work presents a new passive measurement technique that combines acoustic transmission measurements and Lumped-element modelling. For diaphragms of different lengths, compliances between 0.08 ∙ 10-15 and 1.04 ∙ 10-15 m³/Pa are determined without using destructive or complex preparations. In particular, for lengths above 1000 µm, the results differ from numerical simulations only by 4% or less.
Trajectory Recognition and Working Condition Analysis of Rod Pumping Systems Based on Pose Estimation Method with Heatmap-Free Joint Detection
Authors: Zhang Kai , Xia Xiaolong , Song Zewen , Zhang Liming , Yang Yongfei , Wang Jian , Yao Jun , Zhang Huaqing , Zhang Yue , Feng Gaocheng , Liu Chen 
Published: August 2024

Summary
Rod pump systems are the primary production tools in oilfield development. Analyzing their working conditions provides a theoretical foundation for formulating production optimization plans and adjusting equipment parameters. Existing machine learning–based condition analysis methods rely on dynamometer cards and cannot capture the actual operational trajectory of the pumping unit. To address this issue, this paper proposes a keypoint detection method for pumping units based on pose estimation of heatmap-free joint detection from video data. A data annotation scheme suitable for the task of detecting pumping unit keypoints was developed, and the learning criteria for this task were optimized. An end-to-end heatmap-free pose estimation algorithm was used to process images of the pumping unit, yielding predicted keypoint positions, thereby enabling the identification of the keypoint motion trajectories of the pumping unit. Experiments validated the proposed method and compared it with general learning criteria. Results show that this method accurately captures the keypoint positions of the pumping unit, with the optimized learning criteria model improving by more than 5% compared with general methods and increasing the keypoint object keypoint similarity (OKS) by more than 30%. The model’s results can be used for the actual operational trajectory recognition of the pumping unit, automatically calculating the motion parameters of the polished rod, and intelligently assessing the balance and working condition analysis of the pumping unit. This realizes the intelligent application of video surveillance data, significantly contributing to the dynamic study of rod pump systems.
Gut microbiome of Oecophylla smaragdina: potential functions in host and environment
Authors: Agarwal Surbhi , Sharma Garima , Mathur Vartika 
Published: August 2024

Abstract

Oecophylla smaragdina (red weaver ant) is widely consumed either in the form of protein and nutrient rich diet or as a source of medicine provided by traditional healers in various parts of the world. Similar to other animals, ants also form mutualistic relationship with the microbes that live inside them. These endosymbionts perform various crucial functions in their host such as nutritional upgrading, digestion, nitrogen recycling and pathogen defence. However, not much literature is available on the role of gut specific microbes and their functions in the ant host. In this study, we have determined the microbiome profile of O. smaragdina colonies using 16S rRNA sequencing and biochemical characterization for understanding the role of both culturable and non-culturable microbes present in ant gut. While, a number of microbes are reported for the first time, Enterobacteriaceae and Staphylococcaceae were found to be the most dominant families found in O. smaragdina. Some endosymbionts were similar to human gut microbiota depicting the diversity of microbes inside an ant gut. We also focused on the bioactive compounds produced by endosymbionts, which may find an application in the treatment of various ailments in humans.
EFFECT OF CRUDE AQUEOUS AND ETHANOLIC EXTRACTS OF MORINGA (Moringa oleifera) AGAINST COWPEA WEEVILS (Callosobruchus maculatus) IN STORED COWPEA (Vigna unguiculata) IN LOKOJA, KOGI STATE
Authors: Olusanmi Pamilerin Kehinde 
Published: August 2024

Abstract

Background:This study investigated the effect of crude aqueous and ethanolic extracts of Moringa oleifera against Callosobruchus maculatus (cowpea weevil) on stored Vigna unguiculata (Cowpea).The pesticidal activity of Moringa oleifera extracts on Callosobruchus maculatus was evaluated at concentrations of 500, 1000, and 1500g/L. The cold Maceration method was employed in this study. The mortality rate of the weevils was monitored after 24, 48, and 72 hours of bioassay. The mortalities recorded were subjected to ANOVA. The differences between the two extracts were significantly different at P = 0.05.
Results:The inference drawn from this study showed that the crude aqueous extract of Moringa oleifera was not effective against Callosobruchus maculatus on stored cowpea, while the Ethanolic extract showed a significant effect on Callosobruchus maculatus because of the high mortality recorded in the third concentration.
Conclusion:Therefore, Moringa leaf extract is recommended to be used as an alternative to synthetic pesticides for the control of Cowpea weevil, and further research is needed to test the activity of the plant on non-target.
Spatial Structures of Blue Low-Latitude Aurora Observed from Japan during the Extreme Geomagnetic Storm of May 2024
Authors: Nanjo Sota , Shiokawa Kazuo 
Published: August 2024

Abstract

On May 11, 2024, an extreme G5-class geomagnetic storm triggered a spectacular and unusual display of colorful auroras in mid- to low-latitude regions all over the world. In Japan, auroras were seen not only from Hokkaido, the northernmost island, but also from the northern and central parts of Honshu, the main island. The widespread availability of commercial digital cameras among the public led to a flooding of high-resolution auroral images on social media. This study reports on the appearance of a blue aurora during a storm-time substorm on this day and its magnetic field-aligned and longitudinal structures captured by citizen scientists. Observations from two photographers at different locations revealed that the aurora was located at approximately 40 degrees magnetic latitude and magnetic local time (MLT) of 23 h, spanned about 1200 km (1-h MLT) in the longitudinal direction with three separated structures, and ranged in altitude from 400 km to at least 900 km. Simultaneous photometric measurements suggested that this blue aurora likely included emissions at 427.8 nm. While previous studies have noted the existence of emissions at 427.8 nm in low-latitude auroras, this is the first time that a blue low-latitude aurora has been spatially visualized and shown to have longitudinal and field-aligned structures. Additionally, previous studies suggested that blue emissions in low-latitude auroras are produced by energetic neutral atoms from the ring current. However, identifying longitudinal and field-aligned structures in this study may need an alternative explanation.
SET8 inhibition preserves PTEN to attenuate kidney cell apoptosis in cisplatin nephrotoxicity
Authors: Yang Xu , Guan Yingjie , Bayliss George , Zhao Ting C. , Zhuang Shougang 
Published: August 2024

Abstract

The aberrant expression of SET8, a histone methyltransferase that mediates H4 lysine 20 mono-methylation (H4K20me1), is implicated in the pathogenesis of various tumors, however, its role in acute kidney injury (AKI) is unknown. Here we showed that SET8 and H4K20me1 were upregulated in the murine kidney with AKI induced by cisplatin, along with increased renal tubular cell injury and apoptosis and decreased expression of E-cadherin and Phosphatase and Tensin Homolog (PTEN). Suppression of SET8 by UNC0379 improved renal function, attenuated tubule damage, and restored expression of PTEN, but not E-cadherin. UNC0379 was also effective in lessening cisplatin-induced DNA damage response (DDR) as indicated by reduced expression of γ-H2AX, p53, p21, and alleviating cisplatin-impaired autophagy as shown by retained expression of Atg5, Beclin-1, and CHMP2A and enhanced levels of LC3-II in the kidney. Consistently, inhibition of SET8 with either UNC0379 or siRNA mitigated apoptosis and DDR, and restored autophagy, along with PTEN preservation in cultured renal proximal tubular epithelial cell (TKPTs) exposed to cisplatin. Further studies showed that inhibition of PTEN with Bpv or siRNA potentiated cisplatin-induced apoptosis, DDR, and hindered autophagy, and conversely, alleviated by overexpression of PTEN in TKPTs. Finally, blocking PTEN largely abolished the inhibitory effect of UNC0379 on apoptosis. Taken together, these results suggest that SET8 inhibition protects against cisplatin-induced AKI and renal cell apoptosis through a mechanism associated with the preservation of PTEN, which in turn inhibits DDR and restores autophagy.
Induced oocyte maturation in yellowbelly flounder
Authors: Ellis-Smith Brooke , Muncaster Simon 
Published: August 2024

Abstract

Recent interest in developing aquaculture of the endemic New Zealand yellowbelly flounder (YBF) has highlighted the need to develop captive breeding technology for this species. A pilot investigation of induced reproduction was conducted, where twenty-seven female and twelve male wild-caught sexually mature fish were placed into indoor tanks and administered either a sham (saline) or gonadotropin releasing hormone analogue (GnRHa) treatment (50 µg kg-1 or 100 µg kg-1 bw). Ovulation only occurred in GnRHa treated fish. The total number of ovulated fish was low, but the majority of fish treated with 50 µg/kg-1 of GnRHa progressed either into, or through, final oocyte maturation (FOM) to produce hydrated oocytes. Fertilisation rates in excess of 80% were achieved in fish from both the 50 and 100 µg/kg-1 GnRHa treatments, indicating that high quality gametes can be achieved using hormone induced reproduction. In contrast, control fish appeared to arrest oocyte development at FOM suggesting that reproductive dysfunction may occur in wild caught broodstock. With further refinement, induced spawning using GnRHa should be a viable methodology to enhance the hatchery production of YBF.