bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pathogenic characteristics of three genotype II porcine reproductive and respiratory syndrome viruses isolated from China
Authors: Shang Youjun , Wang Guangxiang , Yin Shuanghui , Tian Hong , Du Ping , Wu Jinyan , Chen Yan , Yang Shunli , Jin Ye , Zhang Keshan , Lu Zengjun , Liu Xiangtao 
Published: January 2013

Abstract

Background
We examined differences in pathogenicity in pigs from China that had been experimentally infected with porcine reproductive and respiratory syndrome virus (PRRSV).

Methods
We compared pathogenic characteristics of a field isolate (GX-1/2008F), two PRRSV isolates (HN-1/2008, YN-1/2008) propagated in cells, and GX-1/2008F that had been propagated in cells (GX-1/2008). The clinical courses, along with humoral and cell-mediated responses, were monitored for 21 days post-infection (DPI). Animals were sacrificed and tissue samples used for gross pathological, histopathological and ultrastructure examination.

Results
At 2–3 DPI, animals infected with cell-propagated viruses exhibited signs of coughing, anorexia and fever. However their rectal temperature did not exceed 40.5°C. Viremia was detectable as early as 3 DPI in animals infected with HN-1/2008 and YN-1/2008. Animals inoculated with GX-1/2008F displayed clinical signs at 6 DPI; the rectal temperature of two animals in this group exceeded 41.0°C, with viremia first detected at 7 DPI. Seroconversion for all challenged pigs, except those infected with GX-1/2008, was seen as early as 7 DPI. All of these pigs had fully seroconverted by 11 DPI. All animals challenged with GX-1/2008 remained seronegative until the end of the experiment. Innate immunity was inhibited, with levels of IFN-α and IL-1 not significantly different between control and infected animals. The cytokines IFN-γ and IL-6 transiently increased during acute infection. All virus strains caused gross lesions including multifocal interstitial pneumonia and hyperplasia of lymph nodes. Inflammation of the stomach and small intestine was also observed. Lesions in the group infected with GX-1/2008F were more serious than in other groups. Transmission electron microscopy revealed that alveolar macrophages, plasmacytes and lymphocytes had fractured cytomembranes, and hepatocytes had disrupted organelles and swollen mitochondria.

Conclusions
The pathogenicity of the PRRSV field isolate became attenuated when propagated in MARC-145 cells. Tissue tropism of highly pathogenic strains prevailing in China was altered compared with classical PRRSV strains. The observed damage to immune cells and modulation of cytokine production could be mechanisms that PRRSV employs to evade host immune responses.
Effects of acupuncture treatment on depression insomnia: a study protocol of a multicenter randomized controlled trial
Authors: Chen Yuan-Fang , Liu Jian-Hua , Xu Neng-Gui , Liang Zhao-Hui , Xu Zhen-Hua , Xu Shu-Jun , Fu Wen-Bin 
Published: January 2013

Abstract

Background
More than 70% of patients with depression who see their doctors experience insomnia. Insomnia treatment is a very important link for depression treatment. Furthermore, antidepression treatment is also important for depression insomnia. In acupuncture, LU-7 (Lie Que) and KID-6 (Zhao Hai), which are two of the eight confluence points in meridian theory, are used as main points. An embedded needle technique is used, alternately, at two groups of points to consolidate the treatment effect. These two groups of points are BL-15 (Xin Shu) with BL-23 (Shen Shu) and BL-19 (Dan Shu) with N-HN-54 (An Mian). The effectiveness of these optimized acupuncture formulas is well proven in the practice by our senior acupuncturists in Guangdong Provincial Hospital of TCM. This study has been designed to examine whether this set of optimized clinical formulas is able to increase the clinical efficacy of depression insomnia treatment.

Methods/design
In this randomized controlled multicenter trial, all the eligible participants are diagnosed with depression insomnia. All participants are randomly assigned to one of two groups in a ratio of 1:1 and receive either conventional acupuncture treatment or optimized acupuncture treatment. Patients are evaluated using the Pittsburgh Sleep Quality Index(PSQI)and the Hamilton rating scale(HAMD) for depression. The use of antidepression and hypnotics drugs is also considered. Results are obtained at the start of treatment, 1 and 2 months after treatment has begun, and at the end of treatment. The entire duration of the study will be approximately 36 months.

Discussion
A high quality of trial methodologies is utilized in the study, and the results may provide better evidence for the effectiveness of acupuncture as a treatment for depression insomnia. The optimized acupuncture formula has potential benefits in increasing the efficacy of treating depression insomnia.

Trial registration
The trial was registered in Chinese Clinical Trial Register (ChiCR-TRC-00000481) on 12 August 2009.
Radiotherapy in marginal zone lymphoma
Authors: Deinbeck Kristin , Geinitz Hans , Haller Bernhard , Fakhrian Khashayar 
Published: January 2013

Abstract

Purpose
To evaluate the efficacy of radiotherapy (RT) for early-stage nodal and extranodal marginal zone lymphoma (MZL).

Materials and methods
Patients with stage I (n = 22) and stage II (n = 8) MZL, who were treated with RT were reviewed. The primary tumor localisation was in the orbita (n = 12), stomach (n = 8), head and neck other than the orbita (n = 8), breast (n = 1) and one case of marginal zone lymphoma of the skin (n = 1). The median radiotherapy dose was 40 Gy (5 to 45 Gy).

Results
The median follow-up time was 103 months. The 5-year overall survival and event-free survival rates were 85 ± 7% and 71 ± 9%, respectively. There was no infield recurrence. Recurrence occurred outside of the radiation field in six patients. The relapses were treated with salvage RT and had excellent local control (100%) at five years after salvage RT.

Conclusions
Localized extranodal MZL have an excellent prognosis following moderate-dose RT. RT is also an effective salvage therapy in cases of localized recurrence. Further clinical studies should evaluate the optimal dose for MZL.
Revisiting the evolution of mouse LINE-1 in the genomic era
Authors: Sookdeo Akash , Hepp Crystal M , McClure Marcella A , Boissinot Stéphane 
Published: January 2013

Abstract

Background
LINE-1 (L1) is the dominant category of transposable elements in placental mammals. L1 has significantly affected the size and structure of all mammalian genomes and understanding the nature of the interactions between L1 and its mammalian host remains a question of crucial importance in comparative genomics. For this reason, much attention has been dedicated to the evolution of L1. Among the most studied elements is the mouse L1 which has been the subject of a number of studies in the 1980s and 1990s. These seminal studies, performed in the pre-genomic era when only a limited number of L1 sequences were available, have significantly improved our understanding of L1 evolution. Yet, no comprehensive study on the evolution of L1 in mouse has been performed since the completion of this genome sequence.

Results
Using the Genome Parsing Suite we performed the first evolutionary analysis of mouse L1 over the entire length of the element. This analysis indicates that the mouse L1 has recruited novel 5’UTR sequences more frequently than previously thought and that the simultaneous activity of non-homologous promoters seems to be one of the conditions for the co-existence of multiple L1 families or lineages. In addition the exchange of genetic information between L1 families is not limited to the 5’UTR as evidence of inter-family recombination was observed in ORF1, ORF2, and the 3’UTR. In contrast to the human L1, there was little evidence of rapid amino-acid replacement in the coiled-coil of ORF1, although this region is structurally unstable. We propose that the structural instability of the coiled-coil domain might be adaptive and that structural changes in this region are selectively equivalent to the rapid evolution at the amino-acid level reported in the human lineage.

Conclusions
The pattern of evolution of L1 in mouse shows some similarity with human suggesting that the nature of the interactions between L1 and its host might be similar in these two species. Yet, some notable differences, particularly in the evolution of ORF1, suggest that the molecular mechanisms involved in host-L1 interactions might be different in these two species.
Urease inhibitory activities of β-boswellic acid derivatives
Authors: Golbabaei Sanaz , Bazl Roya , Golestanian Sahand , Nabati Farzaneh , Omrany Zinat Bahrampour , Yousefi Behnam , Hajiaghaee Reza , Rezazadeh Shamsali , Amanlou Massoud 
Published: January 2013

Abstract

Background and the purpose of the study

Boswellia carterii have been used in traditional medicine for many years for management different gastrointestinal disorders. In this study, we wish to report urease inhibitory activity of four isolated compound of boswellic acid derivative.

Methods
4 pentacyclic triterpenoid acids were isolated from Boswellia carterii and identified by NMR and Mass spectroscopic analysis (compounds 1, 3-O-acetyl-9,11-dehydro-β-boswellic acid; 2, 3-O-acetyl-11-hydroxy-β-boswellic acid; 3. 3-O- acetyl-11-keto-β-boswellic acid and 4, 11-keto-β-boswellic acid. Their inhibitory activity on Jack bean urease were evaluated. Docking and pharmacophore analysis using AutoDock 4.2 and Ligandscout 3.03 programs were also performed to explain possible mechanism of interaction between isolated compounds and urease enzyme.

Results
It was found that compound 1 has the strongest inhibitory activity against Jack bean urease (IC50 = 6.27 ± 0.03 μM), compared with thiourea as a standard inhibitor (IC50 = 21.1 ± 0.3 μM).

Conclusion
The inhibition potency is probably due to the formation of appropriate hydrogen bonds and hydrophobic interactions between the investigated compounds and urease enzyme active site and confirms its traditional usage.
Mental health tribunals in England and Wales: a representative's guide
Authors: Jones Chris , Nimmagadda Srikanth , Veitch Paul 
Published: January 2013

SummaryConsultant psychiatrists are familiar with mental health tribunals, at which they appear as key witnesses giving both factual evidence and expert opinion. They also commonly act as the representative of the ‘responsible authority’. The implications of this in terms of roles and responsibilities, and in terms of training and continuing professional development, have received little attention. Psychiatrists should not accept a representative role unless they are sure that they have the necessary competencies and resources – competencies that are alien to most clinicians and resources that are not available in many services. This article outlines those requirements so that psychiatrists can make better-informed decisions about whether or not to undertake the role and provides practical guidance for those who choose to do so.
Clinical staging in severe mental disorder: evidence from neurocognition and neuroimaging
Authors: Lin Ashleigh , Reniers Renate L. E. P. , Wood Stephen J. 
Published: January 2013

SummaryA new approach to understanding severe mental illnesses such as schizophrenia and affective disorders is to adopt a clinical staging model. Such a model defines the extent of the illness such that earlier and milder phenomena are distinguished from later, more impairing features. Part of the appeal of such a model is that it should have cross-diagnostic applications, but to date there has been no attempt to examine imaging or neurocognrtive evidence for staging in this way. We review these two domains of study with particular focus on major depression and bipolar affective disorder. Although there is some support for the staging model in affective disorders, conclusions are limited by the large variability in the clinical samples studied, especially with regard to the presence of psychotic symptoms. We suggest that future research needs to take a transdiagnostic and longitudinal approach.
Declining Second Primary Ovarian Cancer After First Primary Breast Cancer
Authors: Schonfeld Sara J. , de Gonzalez Amy Berrington , Visvanathan Kala , Pfeiffer Ruth M. , Anderson William F. 
Published: January 2013

Purpose Although ovarian cancer incidence rates have declined in the United States, less is known of ovarian cancer trends among survivors of breast cancer. Therefore, we examined second primary ovarian cancers after first primary breast cancer. Methods Data were obtained from the Surveillance, Epidemiology, and End Results program (1973 to 2008). Standardized incidence ratios (SIRs) were calculated as the observed numbers of ovarian cancers among survivors of breast cancer compared with the expected numbers in the general population. Absolute rates were measured as the incidence rates for second primary ovarian cancer by year of diagnosis of the first primary breast cancer adjusted for age of breast cancer diagnosis and years since diagnosis. Results SIRs for second primary ovarian cancer were elevated over the entire study period (SIR, 1.24; 95% CI, 1.2 to 1.3), whereas the absolute rates declined with an estimated annual percentage change near 1% (−1.34% to −0.09% per year). Secular trends for second ovarian cancers were similar after estrogen receptor (ER) –positive and ER-negative breast cancers, whereas the age-specific patterns varied significantly by ER expression (P for interaction < .001). The largest SIR was among women age less than 50 years with ER-negative breast cancer (SIR, 4.35; 95% CI, 3.5 to 5.4). Conclusion Persistently elevated SIRs along with decreasing absolute rates over the entire study period suggest that ovarian cancers in both the general population and survivors of breast cancer are declining in parallel, possibly because of common risk factor exposures. Analytic studies are needed to further assess the parallel overall trends and the age-specific interaction by ER expression.
High Familial Risk in Nodular Lymphocyte-Predominant Hodgkin Lymphoma
Authors: Saarinen Silva , Pukkala Eero , Vahteristo Pia , Mäkinen Markus J. , Franssila Kaarle , Aaltonen Lauri A. 
Published: January 2013

Purpose Nodular lymphocyte-predominant Hodgkin lymphoma (NLPHL) is one of the two established Hodgkin lymphoma (HL) subtypes. The risk factors of NLPHL are largely unknown. In general, genetic factors are known to have a modest effect on the risk of HL; however, familial risk in NLPHL has not been previously examined. We conducted a population-based study by using the Finnish registries and evaluated the familial risk in NLPHL. Patients and Methods We launched a population-based search to identify patients with NLPHL and their relatives by examining the records of the Finnish Cancer Registry, established in 1953, and the official Finnish population registries. We collected a data set of 692 patients with NLPHL, identified their 4,280 first-degree relatives, and calculated the registry-based standardized incidence ratios (SIRs) for different cancers in the first-degree relatives. In addition, the primary tumor biopsies of HL-affected relatives were collected when possible, the HL diagnoses were re-reviewed by a hematopathologist, and the SIR for NLPHL was calculated on the basis of confirmed NLPHL diagnoses. Results On the basis of confirmed NLPHL diagnoses, the SIR for NLPHL was 19 (95% CI, 8.8 to 36) in the first-degree relatives. The risk was most prominent in female relatives of young patients. The registry-based SIR for classical HL was 5.3 (95% CI, 3.0 to 8.8), and for non-Hodgkin lymphoma, it was 1.9 (95% CI, 1.3 to 2.6). Conclusion Our results implicate an unexpectedly high familial component in the development of NLPHL. Research is warranted to identify the putative genetic and environmental factors underlying this finding and to develop strategies for better management of patients with NLPHL and their relatives.
Investigation into High-Temperature Stability of Asphalt Pavement
Authors: Huang Xiaoming , Xu Tao , Zhao Yongli , Li Zhidong 
Published: January 2013

Abstract
To identify potential causes of the unstability problems of asphalt pavement at high temperature, effects of reinforced fiber, asphalt content, and temperature on the stability were analyzed using the wheel tracking test, the triaxial repeated load test (TRT), and partial triaxial test (PTT). The results indicated that Sup19 shows a better rutting resistance and lower permanent deformation than that of AC20, and the polyester fiber has significantly improved the rutting resistance of asphalt mixtures. The asphalt content affects greatly the mixture deformation. PTT method is more sensitive to asphalt content change. High-temperature facilitates to reduce the viscosity of asphalt binder and internal friction between the aggregate particles, resulting in permanent deformation. And most permanent deformation occurs at the first loading cycles because of densification followed by a slight increase in permanent deformation owing to plastic flow. It is concluded that the asphalt content and mixture compaction should be controlled strictly during construction, and fiber can improve the resistance to rutting in pavement, and PTT method can better understand dynamic properties of asphalt mixtures.
Characterization of Asphalt Mixture Homogeneity Based on X-ray Computed Tomography
Authors: Hu Chichun , Youtcheff Jack , Wang Duanyi , Zhang Xiaoning , Kutay Emin , Thyagarajan Senthilmurugan 
Published: January 2013

Abstract
The mechanical performance and life span of asphalt pavement depend largely on the homogeneity of the asphalt mixture. Inhomogeneity of asphalt mixtures is due to the non-uniform segregation of air voids and aggregates in mixtures. In order to evaluate the homogeneity of an asphalt mixture during lab compaction, computed tomography (CT) images of the specimen were obtained based on x-ray CT. The image was virtually cut into slices of various sizes, and the vertical air void distributions and aggregate gradations were quantified. Research results reveal that the air void content in the middle part of the specimen was lower than those on both ends. Also, the homogeneity was better under high compaction levels. The results from aggregate gradations were in good agreement with air void distribution calculations. Therefore, it is feasible to characterize an asphalt mixture’s homogeneity based on the air void distributions and aggregate gradations as determined via x-ray CT.
Production of double haploids in oat (Avena sativa L.) by pollination with maize (Zea mays L.)
Authors: Marcińska Izabela , Nowakowska Agata , Skrzypek Edyta , Czyczyło-Mysza Ilona 
Published: January 2013

AbstractThe aim of the study was to optimize the method of oat haploid production by pollination with maize. Seventeen oat genotypes were used in the experiment. Various factors influencing the growth and development of ovaries and embryo production were investigated: genotype, time of pollination, growth regulators and time of their application. Emasculated before anthesis, oat florets were pollinated with maize pollen after 0, 1 or 2 days. Next, one of two auxins analogues (2,4-D or dicamba) were applied to oat pistils. These auxins had no significant influence on the number of enlarged ovaries and embryos. The time of application of these growth regulators had a significant influence on embryo production. Haploid embryos were obtained from all used genotypes, although the frequency of enlarged ovaries and obtained embryos did not differ markedly between the genotypes. On average, 85% of ovaries were enlarged and 11.7% of them produced haploid embryos. Depending on the regeneration medium, 24–41% of embryos were germinated, of which 12% had developed into green plants. A strong significant difference in the number of germinating embryos and haploid plants was observed between the kind of regenerating medium used. There were no albino plants and all the obtained plants were haploid.
Adult stem cells derived from skeletal muscle — biology and potential
Authors: Danišovič Ľuboš , Polák Štefan , Vojtaššák Ján 
Published: January 2013

AbstractSkeletal muscle contains at least two distinct populations of adult stem cells — satellite cells and multipotent muscle-derived stem cells. Monopotential satellite cells are located under the basal lamina of muscle fibers. They are capable of giving rise only to cells of myogenic lineage, which play an important role in the processes of muscle regeneration. Multipotent muscle-derived stem cells are considered to be predecessors of the satellite cells. Under proper conditions, both in vitro and in vivo, they undergo myogenic, cardiogenic, chondrogenic, osteogenic and adipogenic differentiation. The main purpose of the present article is to summarize current information about adult stem cells derived from skeletal muscle, and to discuss their isolation and in vitro expansion techniques, biological properties, as well as their potential for regenerative medicine.
Nutritional value of breast and thigh muscle of chukar partridge (Alectoris chukar) under intensive fattening conditions
Authors: Jůzl Radovan , Suchý Pavel , Straková Eva , Rusníková Lucie , Macháček Miroslav , Marada Petr 
Published: January 2013

The aim of our study was to evaluate the nutritional value of the breast and thigh muscle of chukar partridge (Alectoris chukar) under intensive fattening conditions. Commercial feeding mixtures for broilers were used. Live weight of 60 partridges (birds were not sexed) was controlled during 90 days of fattening. After the fattening was finished, 30 birds were selected to monitor the carcass yield. Breast and thigh muscle were used for chemical analysis of crude protein (Kjeldahl method), total lipids (fat analyser ANKOMXT10), ash (Muffle furnace - 550° C) and gross energy (calorimetry). Average values ​​of live weight were increasing in the course of fattening; at the end of fattening the live weight reached 0.452 kg. The carcass yield, breast muscle yield and thigh yield were evaluated (73.72%, 18.09%, 20.80%, respectively). Chemical analysis showed a highly significant difference (P ≤ 0.01) between the breast and thigh muscle for crude protein, fat, ash and gross energy. The ash content demonstrated a significant difference (P ≤ 0.05) between the breast and thigh muscle. This study brings new data on the nutritional value of the meat of chukar partridge that can be used for reccomendation of a suitable feeding mixture.