bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bootstrap Evaluation of Association Matrices (BEAM) for Integrating Multiple Omics Profiles with Multiple Outcomes
Authors: Seffernick Anna Eames , Cao Xueyuan , Cheng Cheng , Yang Wenjian , Autry Robert J. , Yang Jun J. , Pui Ching-Hon , Teachey David T. , Lamba Jatinder K. , Mullighan Charles G. , Pounds Stanley B. 
Published: August 2024

AbstractMotivationLarge datasets containing multiple clinical and omics measurements for each subject motivate the development of new statistical methods to integrate these data to advance scientific discovery.ModelWe propose bootstrap evaluation of association matrices (BEAM), which integrates multiple omics profiles with multiple clinical endpoints. BEAM associates a set omic features with clinical endpoints via regression models and then uses bootstrap resampling to determine statistical significance of the set. Unlike existing methods, BEAM uniquely accommodates an arbitrary number of omic profiles and endpoints.ResultsIn simulations, BEAM performed similarly to the theoretically best simple test and outperformed other integrated analysis methods. In an example pediatric leukemia application, BEAM identified several genes with biological relevance established by a CRISPR assay that had been missed by univariate screens and other integrated analysis methods. Thus, BEAM is a powerful, flexible, and robust tool to identify genes for further laboratory and/or clinical research evaluation.AvailabilitySource code, documentation, and a vignette for BEAM are available on GitHub at:https://github.com/annaSeffernick/BEAMR. The R package is available from CRAN at:https://cran.r-project.org/package=BEAMR.ContactStanley.Pounds@stjude.orgSupplementary InformationSupplementary data are available at the journal’s website.
SIP2 functions as the master transcription factor of thePlasmodiummerozoite formation
Authors: Nishi Tsubasa , Kaneko Izumi , Yuda Masao 
Published: August 2024

AbstractMalaria, one of the most serious infectious diseases worldwide, is caused by the proliferation ofPlasmodiumparasites through repeated cycles of intraerythrocytic development. The parasite replicates via schizogony in host erythrocytes, producing multiple progeny merozoites that invade new erythrocytes to continue the intraerythrocytic developmental cycle. Although merozoite formation is the most crucial step in parasite proliferation and malaria pathogenesis, the molecular mechanism regulating merozoite formation remains unclear. SIP2 is an AP2 transcription factor expressed during schizogony and is particularly conserved among erythrocyte-infecting apicomplexan parasites. Here, we reveal that SIP2 inP. berghei(PbSIP2) functions as a transcriptional activator that regulates merozoite formation. Disruption ofpbsip2using a dimerizable Cre recombinase system resulted in developmental arrest before merozoite formation and significant downregulation of merozoite-related genes. ChIP-seq of PbSIP2 showed that it comprehensively activated merozoite-related genes by binding to previously reportedcis-regulatory elements of merozoite invasion-related genes, including the bipartite motif (TGCAN4-6GTGCA). Collectively, our results indicate that SIP2 is a transcription factor that establishes erythrocyte infectivity and may have an evolutionary origin from the common ancestor of erythrocyte-infecting apicomplexan parasites.
Ordering molecular diversity in untargeted metabolomics via molecular community networking
Authors: Coler Elizabeth A. , Melnik Alexey , Lotfi Ali , Moradi Dana , Ahiadu Ben , Portal Gomes Paulo Wender , Patan Abubaker , Dorrestein Pieter C. , Barnes Stephen , Boginski Vladimir , Semenov Alexander , Aksenov Alexander A. 
Published: August 2024

AbstractNature’s molecular diversity is not random but displays intricate organization stemming from biological necessity. Molecular networking connects metabolites with structural similarity, enabling molecular discoveries from mass spectrometry data using arbitrary similarity thresholds that can fracture natural metabolite families. We present molecular community networking (MCN), that optimizes connectivity for each metabolite, rescuing lost relationships and capturing otherwise “hidden” metabolite connections. Using MCN, we demonstrate the discovery of novel dipeptide-conjugated bile acids.
Green human resources in higher education institutions: a systematic literature review
Authors: Saad Zuria Akmal , Fauzi Muhammad Ashraf , Zulkepeli Laili , Moshood Taofeeq Durojaye , Hussain Suhaidah 
Published: August 2024

PurposeThe purpose of this study is to conduct a comprehensive examination of the concept of green human resources management (GHRM) within the context of higher education institutions (HEIs). The GHRM concept has emerged from the broader environmental management concept in the last decade. Its adoption has diffused in many sectors, particularly manufacturing, but empirical studies in HEIs are lacking. HEIs, as the epitome of knowledge creation and dissemination, should lead the GHRM initiative as an example to the corporate world and the public.Design/methodology/approachThe approach applied is based on the systematic literature review approach to draw inferences, analyze and summarize past studies based on the PRISMA method. Several research streams were identified: (1) academic and non-academic GHRM, (2) theoretical foundation, (3) qualitative and quantitative studies, and (4) green training and development.FindingsThis review discovered that GHRM implementation in HEIs leads to better sustainability efforts within academic institutions. It is relevant to stakeholders, policymakers, and HEIs’ top management to strategize in making the academic institution the torchbearer of sustainable practice and a role model for other sectors and communities.Practical implicationsThis study provides implications towards the application of GHRM in HEIs to elevate academic institutions as the torchbearers for sustainability development. The implicit findings based on the research streams produced can benefit future studies in HEIs to adopt best practices in green activities, particularly GHRM. The implication of GHRM in HEIs would impart individual green, cautious, and sustainability practices holistically and comprehensively, improving behavior towards overall higher education activities involving academics, students, administrative staff, and the overall community ecosystem.Originality/valueThis study makes a significant contribution to the existing literature by systematically elucidating the role of GHRM in HEIs, providing insights for academics and policymakers seeking to promote sustainable practices.
Elevated emotions, elevated ideas: the CSR-employee creativity nexus in hospitality
Authors: Ahmad Naveed , Han Heesup , Kim Minseo 
Published: August 2024

PurposeThe competitive nature of the hospitality industry necessitates continual adaptation and innovation. While standardization can stifle creativity, CSR has the potential to enhance employee extra-role behaviors, including creativity. The existing literature on the relationship between CSR and employee creativity is sparse, especially in developing countries, and tends to overlook the importance of emotions. This research was designed to investigate the relationship between CSR and employee creativity in the hospitality sector of an emerging economy, with a focus on the mediating effects of emotions like employee admiration and happiness and the moderating role of employees' altruistic values.Design/methodology/approachData were collected from 428 hospitality employees using questionnaires. Analysis was conducted using the SMART-PLS software.FindingsCSR has a notable influence on creativity. Emotions, specifically employee admiration and happiness, along with personal values, were found to play significant mediating and moderating roles in the CSR-employee creativity relationship.Practical implicationsThe findings provide valuable insights for hospitality managers, suggesting that CSR initiatives can be leveraged to enhance competitive advantages by promoting employee creativity. The study underscores the importance of understanding the emotional and value-based dimensions of employees about CSR initiatives.Originality/valueThis research fills a critical gap in the literature, particularly in the context of emerging economies, by examining the emotional facets of the CSR-employee creativity relationship. The study’s emphasis on emotional mediators and altruistic values as moderators in the said relationship adds a unique dimension to the discourse, enriching the understanding of how CSR can influence hospitality employees' creative outcomes.
Continuous briefing for the future university campus: an evidence-based approach to match spatial supply and demand
Authors: Altınkaya Genel Özlem , den Heijer Alexandra C. , Arkesteijn Monique H. 
Published: August 2024

PurposeTo plan the future university campus, campus executives need decision-making support from theory and practice. Matching the static campus (supply) with the dynamic (demand) - while safeguarding spatial quality and sustainability - requires management information from similar organizations. This study presents an evidence-based briefing approach to support decision-makers of individual universities with management information when making decisions for their future campus.Design/methodology/approachFor the proposed evidence-based briefing approach, the continuous Designing an Accommodation Strategy (DAS) framework is used in a mixed-method research design to evaluate the past to plan for the future. Five campus themes and three campus models (solid, liquid, and gas) are introduced to describe the development and diversification of university campuses and their impact across different university building types. Based on this theoretical framework, first, qualitative interview data are analyzed to understand which standards campus managers expect; second, a quantitative project database is used to demonstrate what is actually realized.FindingsThe findings demonstrate that remote working and online education will become more common. Academic workplaces and learning environments are more adaptive to changes than laboratory spaces. The analyses reveal different effective space use strategies to meet the current demand: they include space-efficient mixed-use buildings, and mono-functional generic educational and office spaces. These results show that operationalized evidence-based briefing can help design the future campus.Originality/valueThe study adds knowledge during a critical (post-COVID) period when decision-makers need evidence from others to adapt their campus management strategies to hybrid and sustainable ambitions.
French Social Democracy in Turmoil
Authors: Marlière Philippe 
Published: August 2024

AbstractFor decades, the Parti socialiste (PS) was the major party of the French left. It elected a president three times and was a ruling party on several occasions between 1981 and 2017, at which point it suffered crushing defeats in the 2017 presidential and legislative elections. Since then, the PS has been between a rock and a hard place: on the left, it is dominated by Jean‐Luc Mélenchon's La France Insoumise (LFI, Unbowed France) and on the right by Macron's Renaissance party. The success of both parties has a lot to do with the PS's own setbacks. Emmanuel Macron's election in 2017 was rendered possible by internal conflicts in the PS during François Hollande's term. Those who thought that Benoît Hamon's candidacy was too left‐wing that year, or those who now regard an alliance with Mélenchon as ‘illegitimate’, found refuge in Macron's party. This article aims to explain the reasons for the PS's dramatic setbacks and to explore its prospects for recovery.
The gut‐immune‐liver axis in patients with alcohol use disorder and clinically low serum zinc levels
Authors: Thakurdesai Aishwarya , Jha Suman K. , Erinkitola Iyabo , Said Aula , Joshi Thwisha , Schwandt Melanie L. , Parajuli Dipendra , Singal Ashwani K. , Kong Maiying , Cave Matthew C. , Vatsalya Vatsalya 
Published: August 2024

AbstractBackgroundAlcohol use disorder (AUD) with chronic and heavy alcohol consumption causes alcohol‐associated liver disease (ALD). Early‐stage ALD exhibits dyshomeostasis of zinc. We investigated the role of zinc deficiency in gut‐barrier dysfunction, proinflammatory response, hepatocyte injury, and death, as well as potential sex differences in AUD patients.MethodsThirty‐nine male and female AUD patients were grouped by normal [≥71 μg/dL (Group 1, number (n) = 26)] and low [<71 μg/dL (Group 2, n = 13)] serum zinc levels. Demographics, alcohol intake markers [Lifetime Drinking History (LTDH), heavy drinking days in the past 90‐days (HDD90), total drinks in the past 90‐days (TD90), number of drinking days in the past 90‐days (NDD90), average drinks per day in the past 90 days (AvgDPD90)] were collected. Blood samples were tested for complete blood count (CBC), comprehensive metabolic panel (CMP), coagulation markers, gut‐barrier dysfunction markers, cytokines, and hepatocyte death markers.ResultsGroup 2 females exhibited lower LTDH than Group 2 males (p = 0.028), but higher recent drinking. Aspartate transaminase: alanine transaminase (AST:ALT) ratio was higher (p = 0.049) in Group 2 males compared to Group 1 males. Overall, Group 2 showed threefold higher interleukin 8 (IL‐8) levels than Group 1 (p = 0.92); these were sevenfold higher in Group 2 females than Group 1 females. Group 2 females also had higher K18M65, but lower K18M30 than Group 1 females. Necrotic type of cell death (K18M65) was well‐described only in Group 2 by the arrangement of lipopolysaccharide (LPS), soluble cluster of differentiation 14 (sCD14), and tumor necrosis factor alpha (TNF‐α) (R2 = 0.633, p = 0.037).ConclusionOur findings demonstrated the role of the gut‐immune‐liver axis in describing hepatocyte injury and death in zinc‐deficient AUD patients. These patients represented an arrangement of gut‐barrier dysfunction and an exacerbated immune response. Shift in the cell‐death mechanism from apoptosis in zinc‐replete females to necrosis in zinc‐deficient females suggests a subclinical to clinical transition of ALD associated with zinc status.
Optogenetic inhibition of light‐captured alcohol‐taking striatal engrams facilitates extinction and suppresses reinstatement
Authors: Vierkant Valerie , Xie Xueyi , Huang Zhenbo , He Lian , Bancroft Eric , Wang Xuehua , Nguyen Tran , Srinivasan Rahul , Zhou Yubin , Wang Jun 
Published: August 2024

AbstractBackgroundAlcohol use disorder (AUD) is a complex condition, and it remains unclear which specific neuronal substrates mediate alcohol‐seeking and ‐taking behaviors. Engram cells and their related ensembles, which encode learning and memory, may play a role in this process. We aimed to assess the precise neural substrates underlying alcohol‐seeking and ‐taking behaviors and determine how they may affect one another.MethodsUsing FLiCRE (Fast Light and Calcium‐Regulated Expression; a newly developed technique which permits the trapping of acutely activated neuronal ensembles) and operant self‐administration (OSA), we tagged striatal neurons activated during alcohol‐taking behaviors. We used FLiCRE to express an inhibitory halorhodopsin in alcohol‐taking neurons, permitting loss‐of‐function manipulations.ResultsWe found that the inhibition of OSA‐tagged alcohol‐taking neurons decreased both alcohol‐seeking and ‐taking behaviors in future OSA trials. In addition, optogenetic inhibition of these OSA‐tagged alcohol‐taking neurons during extinction training facilitated the extinction of alcohol‐seeking behaviors. Furthermore, inhibition of these OSA‐tagged alcohol‐taking neurons suppressed the reinstatement of alcohol‐seeking behaviors, but, interestingly, it did not significantly suppress alcohol‐taking behaviors during reinstatement.ConclusionsOur findings suggest that alcohol‐taking neurons are crucial for future alcohol‐seeking behaviors during extinction and reinstatement. These results may help in the development of new therapeutic approaches to enhance extinction and suppress relapse in individuals with AUD.
Key factors that influence quality of life in patients with IgE‐mediated food allergy vary by age
Authors: Kubala Stephanie A. , Young Fernanda D. , Callier Viviane , Rasooly Marjohn M. , Dempsey Caeden , Brittain Erica , Frischmeyer‐Guerrerio Pamela A. 
Published: August 2024

AbstractBackgroundWhile food allergy (FA) can be fatal, the greatest public health impact of FA arguably lies in its detrimental effect on quality of life (FAQOL). Understanding the factors that contribute to FAQOL at different ages is essential to develop personalized interventions that will improve FAQOL.ObjectiveTo determine the most influential factors that impact FAQOL across ages in well‐phenotyped participants with confirmed FA.MethodsOne hundred and twenty‐five individuals aged 2–28 years with IgE‐mediated FA completed validated age‐specific FAQOL questionnaires. The relationship between demographic/clinical variables and scores were analyzed to identify key predictors of FAQOL.ResultsPoor FAQOL was associated with increasing age, strict avoidance practices, reactions to trace exposures, and more severe reactions as assessed by epinephrine use, anaphylaxis, and/or treatment in the emergency department; FAQOL improved with time from the event. FAQOL was worse in subjects avoiding >2 versus ≤2 foods and in those avoiding milk, egg, soy, sesame, or wheat. Number of foods avoided had greatest impact on children ages 2–7 years, while total number of allergic reactions strongly impacted FAQOL in teens and adults; FAQOL of subjects ages 8–12 years appeared less affected by these variables compared to other age groups. A decision tree analysis identified key predictors of overall FAQOL (age, number of food avoidances, and time since epinephrine use) that can be used to guide intervention strategies to improve FAQOL.ConclusionWe directly compared FAQOL in extensively phenotyped children, teenagers, and adults with confirmed IgE‐mediated FA. Age; timing, number, and severity of reactions; type and number of FA; and food avoidance practices influence FAQOL and should guide intervention strategies.
Pre‐operative pectoralis muscle area index is associated with biomarkers of inflammation and endotoxemia and predicts clinical outcomes after left ventricular assist device implantation: A cohort study
Authors: Carey Matthew R. , Ladanyi Annamaria , Nishikawa Mia , Bordon Abraham , Leb Jay S. , Pinsino Alberto , Driggin Elissa , Latif Farhana , Sayer Gabriel T. , Clerkin Kevin J. , Takeda Koji , Uriel Nir , Colombo Paolo C. , Yuzefpolskaya Melana 
Published: August 2024

AbstractBackgroundPre‐left ventricular assist device (LVAD) pectoralis muscle assessment, an estimate of sarcopenia, has been associated with postoperative mortality and gastrointestinal bleeding, though its association with inflammation, endotoxemia, length‐of‐stay (LOS), and readmissions remains underexplored.MethodsThis was a single‐center cohort study of LVAD patients implanted 1/2015–10/2018. Preoperative pectoralis muscle area was measured on chest computed tomography (CT), adjusted for height squared to derive pectoralis muscle area index (PMI). Those with PMI in the lowest quintile were defined as low‐PMI cohort; all others constituted the reference cohort. Biomarkers of inflammation (interleukin‐6, adiponectin, tumor necrosis factor‐α [TNFα]) and endotoxemia (soluble (s)CD14) were measured in a subset of patients.ResultsOf the 254 LVAD patients, 95 had a preoperative chest CT (median days pre‐LVAD: 7 [IQR 3–13]), of whom 19 (20.0%) were in the low‐PMI cohort and the remainder were in the reference cohort. Compared with the reference cohort, the low‐PMI cohort had higher levels of sCD14 (2594 vs. 1850 ng/mL; p = 0.04) and TNFα (2.9 vs. 1.9 pg/mL; p = 0.03). In adjusted analyses, the low‐PMI cohort had longer LOS (incidence rate ratio 1.56 [95% confidence interval 1.16–2.10], p = 0.004) and higher risk of 90‐day and 1‐year readmissions (subhazard ratio 5.48 [1.88–16.0], p = 0.002; hazard ratio 1.73 [1.02–2.94]; p = 0.04, respectively).ConclusionsPre‐LVAD PMI is associated with inflammation, endotoxemia, and increased LOS and readmissions.
Adipocyte endothelin B receptor activation inhibits adiponectin production and causes insulin resistance in obese mice
Authors: Rivera‐Gonzalez Osvaldo , Mills Megumi F. , Konadu Bridget D. , Wilson Natalie A. , Murphy Hayley A. , Newberry Madison K. , Hyndman Kelly A. , Garrett Michael R. , Webb David J. , Speed Joshua S. 
Published: August 2024

AbstractAimsEndothelin‐1 (ET‐1) is elevated in patients with obesity and adipose tissue of obese mice fed high‐fat diet (HFD); however, its contribution to the pathophysiology of obesity is not fully understood. Genetic loss of endothelin type B receptors (ETB) improves insulin sensitivity in rats and leads to increased circulating adiponectin, suggesting that ETB activation on adipocytes may contribute to obesity pathophysiology. We hypothesized that elevated ET‐1 in obesity promotes insulin resistance by reducing the secretion of insulin sensitizing adipokines, via ETB receptor.MethodsMale adipocyte‐specific ETB receptor knockout (adETBKO), overexpression (adETBOX), or control littermates were fed either normal diet (NMD) or high‐fat diet (HFD) for 8 weeks.ResultsRNA‐sequencing of epididymal adipose (eWAT) indicated differential expression of over 5500 genes (p < 0.05) in HFD compared to NMD controls, and changes in 1077 of these genes were attenuated in HFD adETBKO mice. KEGG analysis indicated significant increase in metabolic signaling pathway. HFD adETBKO mice had significantly improved glucose and insulin tolerance compared to HFD control. In addition, adETBKO attenuated changes in plasma adiponectin, insulin, and leptin that is observed in HFD versus NMD control mice. Treatment of primary adipocytes with ET‐1 caused a reduction in adiponectin production that was attenuated in cells pretreated with an ETB antagonist.ConclusionThese data indicate elevated ET‐1 in adipose tissue of mice fed HFD inhibits adiponectin production and causes insulin resistance through activation of the ETB receptor on adipocytes.
Reverse chewing patterns in patients with bilateral posterior crossbite are related to the occlusal features of the malocclusion
Authors: Piancino Maria Grazia , De Biase Corrado , Di Benedetto Laura , Chaurasia Akhilanand , Vallelonga Teresa , Tortarolo Alessandro 
Published: August 2024

AbstractBackgroundBilateral posterior crossbite (BPXB) is a severe malocclusion associated with maxillary hypoplasia. BPXB may involve the same or a different number of teeth between the sides.ObjectivesTo evaluate the masticatory function in BPXB and the association between the masticatory alterations and the occlusal features of BPXB.MethodsThis observational study included 170 participants: 130 patients with BPXB (65 occlusally symmetric BPXB, i.e. same number and type of posterior teeth in crossbite between the sides, F = 33, M = 32, median age 9.6 (8.2–13) [years.months]; 65 patients occlusally asymmetric BPXB, F = 30, M = 35, median age 9.9 (8.3–13.6)) and 40 controls (F = 25, M = 15, median age 10.2 (9.4–11.6)). The masticatory function was evaluated by the detection of the Reverse Chewing Cycles (RCCs) recorded with a kinesiograph using standardised soft and hard boluses.ResultsThe frequency of RCCs was significantly increased in all BPXB patients compared to controls (p < .000). In symmetric BPXB patients, there were no significant differences in the frequency of RCCs during chewing on the left or the right side. In asymmetric BPXB patients, the frequency of RCCs was significantly increased during chewing on the side with relatively more teeth in crossbite (p < .000).ConclusionsThe masticatory function was significantly altered in all BPXB patients and it was differently affected by symmetric or asymmetric occlusal features of the malocclusion.