bluuebunny/crossref_metadata_2025_split huggingface dataset:
Buccal Fat Pad Excision for Cheek Contouring: A Systematic Review
Authors: Pierzchajlo Noah , Clifton William , Hinson Chandler , Kemeness Catherine , Rogers Toni , Bouillon Victoria N. , Brooks Ronald M. 
Published: July 2024

Introduction: Buccal fat pad (BFP) excision (bichectomy) or lipolysis by injectable deoxycholic acid (brand name, Kybella) is increasingly used procedures for cheek contouring and facial slimming. However, the literature on their effectiveness and safety is inconsistent. The most recent review was published over 5 years ago; however, the study was limited, did not include injectable lipolysis, and available literature has doubled since its publication. Thus, an updated systematic review is needed to provide a comprehensive evaluation of these procedures. Methods: Aligned with the Preferred Reporting Items for Systematic reviews and Meta-analyses (PRISMA) guidelines, a literature review with pre-determined search terms was conducted with 5 major medical journal databases from 1975 to March 2023. We were unable to conduct a meta-analysis due to limited reporting. Results: Our initial search yielded 590 results, with 13 studies meeting criteria for inclusion with a total of 883 patients. All studies performed surgical BFP excision with no studies performing injectable lipolysis. Seven (53%, n = 181) studies reported mean excised volume at 4.01 ± 1.19 grams per side. Two (15%, n = 61) studies assessed mouth opening, noting a combined mean decrease of 28.0 ± 1.93 mm 4 days post-operative and mean decrease of 1.36 ± 4.96 mm 1-month post-operative. Three studies (23%, n = 115) reported a combined mean increase in pain of 2.62 ± 1.63 on a 10-point scale at 72 hours post-operative. Eleven (85%, n = 883) studies reported complications following BFP excision showing complications in 6.8% of patients with 75% of those complications being swelling that resolved spontaneously. Discussion: This review suggests initial patient satisfaction with BFP excision, with only a 6.8% complication rate, primarily consisting of edema that spontaneously resolves. However, concerns regarding bias were raised in 2 of the clinical trials included in this review. Although the original aim was to compare surgical excision to a dissolution technique using deoxycholic acid (DCA), no studies on the DCA were identified that met inclusion criteria. Some authors compared alternative techniques to standard excision, such as hydrodissection and laser-assisted techniques, noting benefits including faster procedures, less pain, and reduced inflammation. Patient age and BFP volume are crucial considerations for appropriate candidate selection, with over excision cautioned against due to natural aging effects. Conclusion: As BFP contouring continues to gain popularity, it is important to continue to monitor outcomes of BFP reduction and its associated complications. It is apparent that more studies are needed in both surgical excision and injectable dissolution to accurately assess this novel procedure.
Biocompatibility and expression of transcription factors of a type B gelatin-Extracellular Matrix of Porcin Urinary Blader scaffold
Authors: Cuevas-Tapia Olivia Abril , Gutiérrez-Sánchez Mariana , Pozos-Guillén Amaury , Cauich-Rodríguez Juan Valerio , Escobar-García Diana María 
Published: July 2024

Objective to evaluate a membrane based on type B gelatin (G) and porcine urinary bladder extracellular matrix (PUB-EM), highlighting the potential effect of the combination evaluated by biocompatibility and regulation of the expression of transcription factors involved in tissue regeneration. G-PUB-EM membranes were prepared at 12.5, 25, and 50% w/v, and evaluated for biocompatibility with Fibroblast. Chemical characterization by FTIR-ATR showed complex spectra during crosslinking process with glutaraldehyde. Physical tests were performed in deionized water and PBS for 48 h. A significant increase in swelling was observed during the first 2 h. Biocompatibility testing (MTS) and evaluation of the expression profile of genes involved in the cell cycle (Cyclin-D1 VEGF, TNF and NF-κ-B) by PCR showed an increase in viability in a PUB-EM content-dependent way, except for 50% PUB-EM membrane which showed cytotoxic effects with a decrease in cell viability below 70%. The membranes showed an increase in the expression of some factors of cell cycle, as well as inflammatory processes that could promote tissue repair. 12.5 and 25% gelatin type B/porcine urinary bladder extracellular matrix (G/PUB-EM) based membranes have potential for tissue regeneration applications. Impact Statement The use of membranes based on type B gelatin and porcine urinary bladder for tissue engineering represents a novel strategy. Biocompatibility and signaling pathways play a primary role in tissue repair and wound recovery. Transcription factors that mediate signaling, cell division and vascularization are part of molecules that intervene in the regenerative potential of cells. These techniques will have a significant impact on tissue repair and regeneration and thus stop depending on tissue donors or other surgical sites from the same patient, as is the case with burn patients.
Spontaneous ovulation in the cat: incidence among queens presented at a veterinary teaching facility
Authors: Pereira Maria Carlos , Schrank Magdalena , Mollo Antonio , Romagnoli Stefano 
Published: July 2024

Objectives The queen is recognised as an induced ovulator. Ovulation without male contact is generally regarded as spontaneous. The aim of this study was to provide an estimate of the incidence of spontaneous ovulation in a population of intact queens presented to a veterinary care facility for both reproductive and non-reproductive reasons. The secondary objective was to determine the roles of age, breed, body weight, presence of tom cats or other cycling queens, and physical contact with humans on triggering spontaneous ovulation, along with its implications. Methods Serum samples from post-pubertal intact queens presented between January 2020 and June 2023 to the Veterinary Teaching Hospital of the University of Padova, Italy, were retrieved and assayed for progesterone (P4) levels. Serum P4 above 2.0 ng/ml without a history of male contact was considered as proof of spontaneous ovulation. Results In total, 31 serum samples from 29 intact post-pubertal queens were obtained. Of the 31 samples, 14 had a P4 concentration above 2.0 ng/ml and 9/29 (31.0%) queens ovulated spontaneously. The mean age and weight of the nine spontaneously ovulating queens were 4.3 ± 5.7 years and 3.7 ± 0.8 kg, respectively. One queen ovulated spontaneously at her first heat at 6 months of age, which makes it the earliest spontaneous ovulation reported. Conclusions and relevance As both our findings and previous publications indicate that the incidence of spontaneous ovulation in queens is consistently ⩾30%, cats should not be considered strictly induced ovulators, but as a species in which ovulation can be either spontaneous or induced. Since the risk of progesterone-dependent conditions (cystic endometrial hyperplasia – pyometra complex, feline mammary hypertrophy) is increased in these queens, veterinarians should be aware and advise breeders and clients accordingly.
Swept-source optical coherence tomography early findings in patients with carotid artery disease
Authors: Monferrer-Adsuara Clara , Remolí-Sargues Lidia , Navarro-Palop Catalina , Cervera-Taulet Enrique , Montero-Hernández Javier , Medina-Bessó Pascual , Castro-Navarro Verónica 
Published: July 2024

Purpose Ocular ischemic syndrome can be the first and only hint of life-threatening carotid artery disease. The early recognition of carotid stenosis-related retinal signs, as well as the comprehension of the pathophysiology behind retinal changes could become relevant for physicians to predict the risk of stroke. The aim of this study is to assess the carotid artery disease-induced early structural retinochoroidal changes by means of swept-source optical coherence tomography (SS-OCT). Methods A prospective observational study was conducted in 72 eyes with carotid stenosis. According to the degree of stenosis, the participants were divided into a normal group (34 eyes), a mild-moderate stenosis group (22 eyes), a severe stenosis group (16 eyes). SS-OCT and OCTA were performed to scan macular fovea. Central macular thickness (CMT), subfoveal choroidal thickness (SCT) and foveal avascular zona (FAZ) area were the major measurements for our study. Results CMT was significantly thicker in group 3 when compared to group 2 and 1. SCT was significantly thinner in group 3 vs group 1, being thicker in group 2 when compared to group 1. No significant differences were obtained when comparing FAZ in the superficial and middle capillary plexus although it was significant when comparing the FAZ in the deep capillary plexus between group 1 and 3. Conclusion internal carotid artery stenosis greater than 70% leads to a significant increase in CMT and a decrease in SCT prior the development of clinical findings of ocular ischemia syndrome.
Measurement of the indoor environment and heating energy consumption of a passive office building in severely cold region, China
Authors: Wang Zhaojun , Liu Chang , Yao Peng , Fu Xiaopan 
Published: July 2024

Passive ultra-low energy buildings represent an effective strategy for energy conservation and emission reduction within the global building industry. The prolonged and cold winters in severely cold regions of China necessitate substantial heating energy consumption. The study utilized a combination of overall surveys and long-term tracking surveys to evaluate the indoor environment and energy consumption in a passive office building situated in severely cold region under different heating modes over 2 years. The results show that the average temperature and indoor air quality (IAQ) can meet the standards at most moments. However, the average relative humidity tends to fall below the specified lower limit. Nevertheless, the Predicted Mean Vote (PMV) index suggests that the indoor environment provides a comfortable thermal experience for humans. The heat pump air conditioning (HPAC) system operation revealed that when the outdoor air temperature fell below −7°C, the coefficient of performance (COP) of the air source heat pump units would deteriorate. Implementing intermittent heating during the second year can reduce heating energy consumption by 13.4 kWh/(m2·a), resulting in 38.9% energy savings. These findings will serve as a valuable reference for the design and operation of heating systems in passive buildings in severely cold regions.
A technology facilitated platform for addressing opioid risks and promoting resilience following traumatic injury in young people
Authors: Adams Zachary W , Marriott Brigid R , Karra Swathi , Linhart-Musikant Elizabeth , Raymond Jodi L , Magee Lauren , Ortiz Damaris , Bell Teresa M 
Published: July 2024

Introduction Traumatically injured young people are at elevated risk for opioid and other substance use following discharge from the hospital. There are few scalable procedures in place for monitoring patients’ behavioral health recovery and connecting them to needed services. This study piloted an adapted version of an existing technology-facilitated program to monitor substance use and connect patients to care (Trauma Resilience and Recovery Program). Methods Participants aged 12–30 years ( n = 90) were enrolled from Level 1 trauma centers and invited to engage with the adapted Trauma Resilience and Recovery Program. The program involved app-based educational content, daily text message symptom monitoring, periodic online screenings, and referral to behavioral health services. Descriptive analyses were performed to characterize the sample and engagement in the program. Results Although interaction with app-based educational content was low (15% of participants), engagement in text-based monitoring (participants responded to 65% of prompts) and mental health screens (65% of participants completed all screens) was high. Rates of substance use varied across time points. Elevated depression, anxiety, and post-traumatic stress disorder symptoms were also observed. Most (85%) participants whose screener responses indicated likely presence of behavioral health concerns participated in clinician referral conversations. Conclusion The enhanced Trauma Resilience and Recovery Program content and procedures were effective in identifying young people experiencing substance use and mental health concerns following traumatic injury and illness. Future work is needed to improve the engagement with educational content and test the effectiveness of the enhanced Trauma Resilience and Recovery Program model in improving substance-related clinical outcomes for trauma-affected young people.
Blood Pressure Fluctuation During 72 Hours After Endovascular Therapy and Prognosis in Acute Ischemic Stroke Patients
Authors: Tao Mingfeng , Li Yongxin , Peng Ya , Zhang Xin , Liu Sheng , Tang Tieyu , Xu Tian , Ke Kaifu 
Published: July 2024

Purpose: Our study aimed to investigate the relationship between fluctuations in different blood pressure (BP) components within 72 hours following endovascular therapy (EVT) and the prognosis of acute ischemic stroke (AIS) patients. Methods: This prospective multicenter study included 283 AIS patients who underwent EVT and had available BP data. The primary outcome was the ordinal modified Rankin Scale (mRS) score evaluated at 90 days. The secondary outcome was a combination of death and major disability, defined as an mRS score of 3 to 6 within 3 months. Results: After adjusting for imbalanced variables, the highest tertile of systolic blood pressure (SBP) fluctuation had an odds ratio (OR) of 1.747 (95% confidence interval [CI]=1.031-2.961; p for trend=0.035) for the primary outcome and 1.889 (95% CI=1.015-3.516; p for trend=0.039) for the secondary outcome, respectively. Fluctuations in diastolic blood pressure (DBP) (OR=1.914, 95% CI=1.134-3.230, p for trend=0.015) and mean arterial pressure (MAP) (OR=1.759, 95% CI=1.026-3.015, p for trend=0.039) were only associated with the primary outcome. The multivariate-adjusted restricted cubic spline analyses supported these findings. Furthermore, the fluctuations in both SBP and MAP exhibited the significant discriminatory capability in predicting the prognosis, comparable to their mean values. Conclusion: Our study revealed that larger fluctuations in SBP, DBP, and MAP within 72 hours after EVT were associated with a higher risk of poor clinical outcomes within 3 months in AIS patients. Controlling BP fluctuations may be valuable for improving the prognosis in patients undergoing EVT. Clinical Impact How will this change clinical practice? It provides physicians a new approach to directly monitor BP fluctuations over an extended observation period in AIS patients after EVT in routine clinical practice. What does it mean for the clinicians? These results underscore the importance of giving equal attention to controlling long-term BP fluctuations, in addition to managing mean BP, as a means to improve the prognosis of AIS patients after EVT. What is the innovation behind the study? This study systematically evaluated the association between fluctuations in different blood pressure components and clinical outcomes in AIS patients over an extended period following EVT.
Focal pyomyositis caused by Enterobacter in an immunocompetent patient: A case report
Authors: Tounsi Haifa , Jlidi Mohamed , Skouri Wafa , Bachrouch Sabrine , Alaya Zeineb , Farhat Emna , Sbaihi Siwar , Bachalli Asma , Amri Raja 
Published: July 2024

Pyomyositis is a pyogenic infection of skeletal striated muscle, usually found in tropical areas, often in immunocompromised patients. We report a new observation of a nontropical Enterobacter pyomyositis occurring in an immunocompetent female in Tunisia. A 53-year-old patient presented with acute fever and intense myalgia in the right thigh. On clinical examination she had an altered general condition, a fever at 40°C and an important swelling of the lateral side of the right thigh. In biology, she had an inflammatory syndrome. Blood culture had identified Enterobacter. Muscle magnetic resonance imaging showed diffuse inflammatory involvement of the vastus lateralis muscle of the right quadriceps associated with edematous infiltration of subcutaneous fatty tissues. Diagnosis of pyomyositis was retained. Antibiotic therapy initially probabilistic and then adapted to the antibiogram was initiated with a favorable outcome. Although rare outside the tropics, the potential severity of pyomyositis encourages its better knowledge.
Advances in Surgery for Metastatic Disease of the Spine: An Update for Oncologists
Authors: Kow Chien Yew , Castle-Kirszbaum Mendel , Kam Jeremy KT , Goldschlager Tony 
Published: July 2024

Study Design Narrative review Objective Metastatic spine disease is an increasingly common clinical challenge that requires individualised multidisciplinary care from spine surgeons and oncologists. In this article, the authors describe the recent surgical advances in patients presenting with spinal metastases. Methods We present an overview of the presentation, assessment, and management of spinal metastases from the perspective of the spine surgeon, highlighting advances in surgical technology and techniques, to facilitate multidisciplinary care for this complex patient group. Neither institutional review board approval nor patient consent was needed for this review. Results Advances in radiotherapy delivery and systemic therapy (including immunotherapy and targeted therapy) have refined operative indications for decompression of neural structures and spinal stabilisation, while advances in surgical technology and technique enable these goals to be achieved with reduced morbidity. Formulating individualised management strategies that optimise outcome, while meeting patient goals and expectations, requires a comprehensive understanding of the factors important to patient management. Conclusion Spinal metastases require prompt diagnosis and expert management by a multidisciplinary team. Improvements in systemic, radiation, and surgical therapies have broadened operative indications and increased operative candidacy, and future advances are likely to continue this trend.
Genome-wide identification, phylogeny and expression analysis of Hsf gene family in Verbena bonariensis under low-temperature stress
Authors: Yang Xiuliu , Wang Sisi , Cai Ju , Zhang Tao , Yuan Dandan , Li Yan 
Published: July 2024

Abstract
Background
The heat shock transcription factor (Hsf) is a crucial regulator of plant stress resistance, playing a key role in plant stress response, growth, and development regulation.

Results
In this study, we utilized bioinformatics tools to screen 25 VbHsf members, which were named VbHsf1-VbHsf25. We used bioinformatics methods to analyze the sequence structure, physicochemical properties, conserved motifs, phylogenetic evolution, chromosome localization, promoter cis-acting elements, collinearity, and gene expression of Hsf heat shock transcription factor family members under low-temperature stress. The results revealed that the majority of the Hsf genes contained motif1, motif2, and motif3, signifying that these three motifs were highly conserved in the Hsf protein sequence of Verbena bonariensis. Although there were some variations in motif deletion among the members, the domain remained highly conserved. The theoretical isoelectric point ranged from 4.17 to 9.71, with 21 members being unstable proteins and the remainder being stable proteins. Subcellular localization predictions indicated that all members were located in the nucleus. Phylogenetic analysis of the Hsf gene family in V. bonariensis and Arabidopsis thaliana revealed that the Hsf gene family of V. bonariensis could be categorized into three groups, with group A comprising 17 members and group C having at least two members. Among the 25 Hsf members, there were 1–3 exons located on seven chromosome fragments, which were unevenly distributed. Collinearity analysis demonstrated the presence of seven pairs of homologous genes in the VbHsf gene family. The Ka/Ks ratios were less than one, indicating that the VbHsf gene underwent purification selection pressure. Additionally, nine genes in V. bonariensis were found to have collinearity with A. thaliana. Promoter analysis revealed that the promoters of all VbHsf genes contained various types of cis-acting elements related to hormones and stress. Based on RNA-seq data, qRT-PCR analysis of six highly expressed genes was performed, and it was found that VbHsf5, VbHsf14, VbHsf17, VbHsf18, VbHsf20 and VbHsf21 genes were highly expressed at 12 h of low-temperature treatment, and the expression decreased after 24 h, among which VbHsf14 was up-regulated at 12 h of low-temperature by 70-fold.

Conclusions
Our study may help reveal the important roles of Hsf in plant development and show insight for the further molecular breeding of V. bonariensis.
guidedNOMe-seq quantifies chromatin states at single allele resolution for hundreds of custom regions in parallel
Authors: Schwaiger Michaela , Mohn Fabio , Bühler Marc , Kaaij Lucas J. T. 
Published: July 2024

AbstractSince the introduction of next generation sequencing technologies, the field of epigenomics has evolved rapidly. However, most commonly used assays are enrichment-based methods and thus only semi-quantitative. Nucleosome occupancy and methylome sequencing (NOMe-seq) allows for quantitative inference of chromatin states with single locus resolution, but this requires high sequencing depth and is therefore prohibitively expensive to routinely apply to organisms with large genomes. To overcome this limitation, we introduce guidedNOMe-seq, where we combine NOMe profiling with large scale sgRNA synthesis and Cas9-mediated region-of-interest (ROI) liberation. To facilitate quantitative comparisons between multiple samples, we additionally develop an R package to standardize differential analysis of any type of NOMe-seq data. We extensively benchmark guidedNOMe-seq in a proof-of-concept study, dissecting the interplay of ChAHP and CTCF on chromatin. In summary we present a cost-effective, scalable, and customizable target enrichment extension to the existing NOMe-seq protocol allowing genome-scale quantification of nucleosome occupancy and transcription factor binding at single allele resolution.