bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryptococcosis—a systematic review to inform the World Health Organization Fungal Priority Pathogens List
Authors: Dao Aiken , Kim Hannah Yejin , Garnham Katherine , Kidd Sarah , Sati Hatim , Perfect John , Sorrell Tania C , Harrison Thomas , Rickerts Volker , Gigante Valeria , Alastruey-Izquierdo Ana , Alffenaar Jan-Willem , Morrissey C Orla , Chen Sharon C-A , Beardsley Justin 
Published: June 2024

AbstractCryptococcosis causes a high burden of disease worldwide. This systematic review summarizes the literature on Cryptococcus neoformans and C. gattii infections to inform the World Health Organization’s first Fungal Priority Pathogen List. PubMed and Web of Science were used to identify studies reporting on annual incidence, mortality, morbidity, antifungal resistance, preventability, and distribution/emergence in the past 10 years. Mortality rates due to C. neoformans were 41%–61%. Complications included acute renal impairment, raised intracranial pressure needing shunts, and blindness. There was moderate evidence of reduced susceptibility (MIC range 16–32 mg/l) of C. neoformans to fluconazole, itraconazole, ketoconazole, voriconazole, and amphotericin B. Cryptococcus gattii infections comprised 11%–33% of all cases of invasive cryptococcosis globally. The mortality rates were 10%–23% for central nervous system (CNS) and pulmonary infections, and ∼43% for bloodstream infections. Complications described included neurological sequelae (17%–27% in C. gattii infections) and immune reconstitution inflammatory syndrome. MICs were generally low for amphotericin B (MICs: 0.25–0.5 mg/l), 5-flucytosine (MIC range: 0.5–2 mg/l), itraconazole, posaconazole, and voriconazole (MIC range: 0.06–0.5 mg/l). There is a need for increased surveillance of disease phenotype and outcome, long-term disability, and drug susceptibility to inform robust estimates of disease burden.
FATTY ACID DESATURASE4 enhances plant RNA virus replication and undergoes host vacuolar ATPase-mediated degradation
Authors: Fang Xinxin , Jia Zhaoxing , Yu Tianqi , Rui Penghuan , Zheng Hongying , Lu Yuwen , Peng Jiejun , Rao Shaofei , Wu Jian , Chen Jianping , Yan Fei , Wu Guanwei 
Published: June 2024

Abstract
Emerging evidence indicates that fatty acid (FA) metabolic pathways regulate host immunity to vertebrate viruses. However, information on FA signaling in plant virus infection remains elusive. In this study, we demonstrate the importance of fatty acid desaturase (FAD), an enzyme that catalyzes the rate-limiting step in the conversion of saturated FAs into unsaturated FAs, during infection by a plant RNA virus. We previously found that the rare Kua-ubiquitin-conjugating enzyme (Kua-UEV1) fusion protein FAD4 from Nicotiana benthamiana (NbFAD4) was downregulated upon turnip mosaic virus (TuMV) infection. We now demonstrate that NbFAD4 is unstable and is degraded as TuMV infection progresses. NbFAD4 is required for TuMV replication, as it interacts with TuMV replication protein 6K2 and colocalizes with viral replication complexes. Moreover, NbFAD4 overexpression dampened the accumulation of immunity-related phytohormones and FA metabolites, and its catalytic activity appears to be crucial for TuMV infection. Finally, a yeast 2-hybrid library screen identified the vacuolar H+-ATPase component ATP6V0C as involved in NbFAD4 degradation and further suppression of TuMV infection. This study reveals the intricate role of FAD4 in plant virus infection, and sheds light on a new mechanism by which a V-ATPase is involved in plant antiviral defense.
Autocratization Spillover: When Electing an Authoritarian Erodes Election Trust across Borders
Authors: Chan Ka Ming 
Published: June 2024

Abstract
The rich literature on election trust predominantly uses domestic determinants as explanatory factors. But given the international nature of the autocratization wave, can an autocratization event across borders erode election trust? This article argues that an authoritarian’s electoral success in a neighboring country can shatter democratic norms and demonstrate the viability of authoritarians. This autocratization event abroad can thus reduce citizens’ principled support for democracy and its political system. Consequently, citizens across borders are less likely to see democratic elections as the “only game in town” and they have less trust in elections. To test this idea, I study the spillover effects of the 2018 Brazilian presidential election, in which an authoritarian candidate won decisively. Using the AmericasBarometer in Colombia that was launched throughout this election, I find that the election trust of Colombian citizens erodes after Jair Bolsonaro’s electoral success. To probe into the mechanism, I uncover that his electoral victory leads Colombian citizens to be less supportive of the domestic political system and reduces their principled support for democracy. The causal mediation analysis demonstrates that these two variables mediate the effect of the authoritarian’s electoral success on election trust. These findings on autocratization spillover effects illustrate the importance of external autocratization events in the study of election trust.
Association of psychological stress with wives’ hypertension across over 10 million Chinese married female population aged 20–49 years
Authors: Zhao Zhenyan , Jia Jiajing , Lyu Xinyi , Zhang Lihua , Wang Yuanyuan , He Yuan , Peng Zuoqi , Zhang Ya , Zhang Hongguang , Wang Qiaomei , Shen Haiping , Zhang Yiping , Yan Donghai , Ma Xu , Yang Ying 
Published: June 2024

Abstract

Background:
Psychological stress has been reported to be a potential risk factor for hypertension among females, but it remains unclear whether spousal chronic stress levels alter the risk of hypertension among women. We examined the associations between stress within the family and hypertension among married women.

Methods:
Reproductive-aged women who were planning for pregnancy and their husbands were recruited from the National Free Pre-pregnancy Checkup Projects (NFPCP) across 31 provinces in China in 2016 and 2017. Perceived stress of wives or husbands was measured with a 5-point Likert-type scale, and assessed from three domains: work/life-related stress, economic stress, and overall stress. Multivariable-adjusted logistic regression models were used to assess the associations between stress status and the prevalence of hypertension.

Results:
Of 10,027,644 couples, 261,098 (2.60%) women had hypertension. The results showed that higher stress levels among themselves or their husbands were associated with a higher prevalence of hypertension in women (P
for trend <0.001). Compared with non-stressed participants, female participants with the highest stress themselves were at a greater risk of hypertension, with adjusted odds ratio (OR) of 1.31 (95% confidence interval [CI]: 1.25–1.37); and compared with participants whose husbands had no stress, those whose husbands had the highest stress level were at a higher risk of hypertension with adjusted OR of 1.24 (95% CI: 1.20–1.29). Moreover, compared with non-stressed status for both couples, only-wife-stressed, only-husband-stressed, and both-stressed couples were found to be significantly associated with increased risks of wives’ hypertension, with adjusted ORs of 1.28 (95% CI: 1.25–1.31), 1.19 (95% CI: 1.17–1.21), and 1.28 (95% CI: 1.26–1.31), respectively.

Conclusion:
Moderate to severe stress in both spouses might be associated with female hypertension prevalence, which highlights the importance of paying attention to the psychological stresses of couples within the family.
Cardiovascular health in people with perinatally acquired HIV – where do we stand?
Authors: Majonga Edith D. , Henderson Merle , Ferrand Rashida A. 
Published: June 2024

Purpose of review
HIV-associated cardiac disease was well recognized in the preantiretroviral (ART) era among children with perinatally-acquired HIV infection (PHIV). While ART has dramatically improved survival, it has become increasingly apparent that individuals with PHIV continue to experience multisystem co-morbidities. We review the cardiac and vascular manifestations in people growing up with PHIV in the ART era.

Recent findings
ART has resulted in a drop in incidence of serious cardiac morbidity. However, there is a substantial body of evidence that demonstrates that cardiac and vascular structural and functional abnormalities, mostly subclinical, are common in people with PHIV taking ART. Studies have considerable heterogeneity with respect to types of cardiovascular assessments used. HIV-mediated chronic inflammation and potentially effects of ART contribute to these abnormalities. The long-term clinical significance of these abnormalities remains unknown as studies have mainly been cross-sectional, but it is likely that the burden of cardiovascular disease will grow as individuals with PHIV age and the prevalence of traditional risk factors increases.

Summary
Understanding the pathogenesis of cardiovascular disease in PHIV, is critical to inform screening and interventional strategies. Longitudinal studies are also needed to understand the natural history of cardiovascular abnormalities and incidence of clinical outcomes.
Patient education and surgical decision-making in genital gender-affirming surgery
Authors: Arteaga Rebecca , Dryden Kim , Blasdel Gaines 
Published: June 2024

Purpose of review
An update on the latest advances in surgical decision-making for genital gender-affirming surgery (GGAS), with an emphasis on patient education from three perspectives: surgeon-team perspectives, patient perspectives, and educational resources available to the public on social media.

Recent findings
Transgender, nonbinary, and other culturally specific gender identities (TGNB) patients overwhelmingly desire an active role in the decision-making process. New developments in patient-engagement strategies help mitigate the harm of historical gatekeeping practices by centering the needs and experiences of TGNB patients to create a prioritized research agenda for GGAS effectiveness. Patient educational resources play an integral role in navigating the complexity of GGAS-surgical options, informing patients of the technical and logistical knowledge required for GGAS surgical decision-making. Peer support experiences are a critical facilitator for patient surgical decision-making, both for informed decision-making and for psychosocial support.

Summary
Several developments have been made in examining shared-decision making, educational resources from the perspectives of both the surgical team and patients, and the role of social media in GGAS. Surgeons must continue to initiate robust conversations of patient goals, GGAS surgical options, the possible risk and benefit profiles of each option, and aftercare requirements, all while prioritizing patient-engagement strategies.
Focal Shock Waves Increase Efficacy and Prolong the Effect of Botulinum Toxin on Spasticity in Patients With Brain Injury From Stroke and Multiple Sclerosis
Authors: Déniz Antonio , Saavedra Pedro , Marrero Isabel , Hernández Jaime 
Published: June 2024

Objective
The aim of the study is to assess the effects on spasticity reduction of the association between focal extracorporeal shock wave therapy and botulinum toxin type A, versus the toxin only in brain injury patients.

Design
Eighteen patients were included. The study had two phases: the first phase was observational, and botulinum toxin type A was used. The second was a prospective, deliberate intervention phase in which the toxin was injected and focal extracorporeal shock wave treatment was added (1 sessions/week, for 3 wks). The patients were followed up in the 1st, 4th, and 6th month, the Ashworth Scale criterion was applied, and for those with lower limb involvement and changes in walking, the 10-meter walk test was used.

Results
Patients treated with toxin only showed a statistically significant improvement in spasticity, with 1 point on the Ashworth Scale from week 5, which disappeared at week 17. However, the combined therapy reduced spasticity by 2 points from week 1 to week 25 (P < 0.001), with a faster result in the 10-meter gait test (P = 0.004).

Conclusions
Combined and simultaneous treatment with botulinum toxin and focal extracorporeal shock wave reduced spasticity in a more effective and prolonged way than treatment with botulinum toxin only.
In Search of a Common Language: The Standardized Electrode Nomenclature for Stereoelectroencephalography Applications
Authors: Chiu Michelle Y. , Bolton Jeffrey , Raskin Jeffrey S. , Curry Daniel J. , Weiner Howard L. , Pearl Phillip L. , Stone Scellig 
Published: June 2024

Purpose:
Stereoelectroencephalography (SEEG) is widely performed on individuals with medically refractory epilepsy for whom invasive seizure localization is desired. Despite increasing adoption in many centers across the world, no standardized electrode naming convention exists, generating confusion among both clinical and research teams.

Methods:
We have developed a novel nomenclature, named the Standardized Electrode Nomenclature for SEEG Applications system. Concise, unique, informative, and unambiguous labels provide information about entry point, deep targets, and relationships between electrodes. Inter-rater agreement was evaluated by comparing original electrode names from 10 randomly sampled cases (including 136 electrodes) with those prospectively assigned by four additional blinded raters.

Results:
The Standardized Electrode Nomenclature for SEEG Application system was prospectively implemented in 40 consecutive patients undergoing SEEG monitoring at our institution, creating unique electrode names in all cases, and facilitating implantation design, SEEG recording and mapping interpretation, and treatment planning among neurosurgeons, neurologists, and neurophysiologists. The inter-rater percent agreement for electrode names among two neurosurgeons, two epilepsy neurologists, and one neurosurgical fellow was 97.5%.

Conclusions:
This standardized naming convention, Standardized Electrode Nomenclature for SEEG Application, provides a simple, concise, reproducible, and informative method for specifying the target(s) and relative position of each SEEG electrode in each patient, allowing for successful sharing of information in both the clinical and research settings. General adoption of this nomenclature could pave the way for improved communication and collaboration between institutions.
Barriers and facilitators for the implementation of co-created nursing innovations in a Dutch university hospital
Authors: van der Zanden Myrthe , Helder Onno , Westland Heleen , Ista Erwin 
Published: June 2024

ABSTRACT

Introduction:
Escalating global nursing shortages require solutions for efficient care, fewer injuries and lost workdays. When commercial solutions are lacking, innovations developed through co-creation can be a viable alternative. However, many co-created nursing innovations are ineffectively implemented. Understanding the barriers and facilitators for the successful implementation of co-created nursing innovations is crucial.

Objective:
This study explored nurses’ perceived determinants influencing the implementation of co-created nursing innovations.

Methods:
We conducted a qualitative study using semi-structured interviews with nurses at a Dutch university hospital who were working with co-created nursing innovations. The Theoretical Domains Framework (TDF) was employed to identify the barriers and facilitators. Data analysis followed a deductive theoretical thematic analysis approach based on the 14 TDF domains. Determinants were labeled as a barrier, facilitator, or both.

Results:
Of the 14 TDF domains, 9 appeared relevant, including Intention, Professional Role and Identify, and Knowledge. The absence of a “superuser,” lack of storage space, and negative experiences during use were perceived as barriers to the implementation of nursing innovations. The importance of time-saving features, user-friendliness, reminders to use the innovation, and team enthusiasm were perceived as facilitators.

Conclusions:
Participants confirmed that the Social Influences domain, combined with Behavioral Regulation and Knowledge, were barriers to implementation, while Intentions and Reinforcements and Rewards were seen as facilitators.

Spanish abstract:

http://links.lww.com/IJEBH/A235
IntegrIBS: Evaluating the Potential and Challenges of Building a Robust IBS Classifier with Integrated Microbiome Data
Authors: Palani Dinakaran , Bapatdhar Nishad , Manchiraju Renuka , Kumar Bipin Pradeep , Ghosh Samik , Palaniappan Sucheendra K. 
Published: June 2024

AbstractIrritable Bowel Syndrome (IBS) is a condition that is quite complicated and shares its symptoms with other related diseases, making it difficult to diagnose. In this study, we initially trained machine learning models on individual microbiome datasets and tested their performance on other datasets, observing variability and low precision among them. To mitigate this, we hypothesised that integrating multiple publicly available microbiome datasets will capture a wide spectrum of microbiome variations across different geographies and demographics. Utilizing this integrated dataset, the XGBoost model achieved a mean accuracy of 0.75 with a standard deviation of 0.04 in 10-fold cross-validation, demonstrating its potential for robust IBS prediction. Explainability analysis identified key bacterial taxa influencing predictions, aligning with existing literature. However, the model’s performance declined significantly when using a leave-one-dataset-out approach, where the model was trained on all but one dataset and tested on the excluded dataset. The results highlight the challenges of generalizing across diverse datasets due to biological and technical variability. These findings present a cautionary tale regarding the integration of datasets and interpretation of results, emphasizing the need for more comprehensive approaches to develop reliable diagnostic tools for IBS.
Joint Model with Random Changepoint for Longitudinal Measures and Semi-competing Risks
Authors: Wang Xuzhi , Larson Martin G. , Tripodis Yorghos , LaValley Michael P. , Liu Chunyu 
Published: June 2024

AbstractDementia displays a gradual decline in cognitive abilities, often accompanied by an accelerated cognitive decline preceding diagnosis. Changepoint models are proposed to identify when cognitive decline accelerates and how it progresses. Joint models are developed to further account for dropout due to death or dementia. Cognitive decline in dementia patients may lead to complications that have an impact on their mortality. However, few joint models consider semi-competing risks (i.e., dementia and death) by distinguishing transitions between various health states, i.e., dementia without death, death after dementia, and death without dementia. We proposed a joint model that accounts for both changepoints and semi-competing risks by combining a multivariate random changepoint model for cognitive decline with an illness-death model that estimates health state transitions. We examined the proposed model with two types of random changepoints: one with a smooth change and another with an abrupt change. We also explored a shared random effect structure and a current value structure that connect both longitudinal and survival processes. Two types of cohorts, i.e., a disease cohort and a community cohort, were generated to evaluate the models. Simulation studies showed our proposed models could effectively characterize the influence of the longitudinal process on health state transitions. In addition, the choice of changepoint formulations, association structures, and cohort types impacted model performance. Real data application in the Framingham Heart Study indicated significant associations between changepoints in cognitive trajectories and health states for dementia and death. Our method provides a flexible framework to integrate longitudinal trajectories with changepoints and semi-competing risks.
Bayesian Joint Model with Latent Time Shifts for Multivariate Longitudinal Data with Informative Dropout
Authors: Wang Xuzhi , Larson Martin G. , Tripodis Yorghos , LaValley Michael P. , Liu Chunyu 
Published: June 2024

AbstractDementia often has an insidious onset with considerable individual differences in disease manifestation. Nonlinear mixed-effects models with latent time shifts have been proposed to investigate the long-term disease progression and individual disease stages. The latent time shift is a horizontal shift in time that aligns patients along a global timeline for disease progression. However, these models ignore informative dropout due to dementia or death, which may result in biased estimates of the longitudinal parameters. To account for informative dropout due to dementia or death, we propose a multivariate nonlinear joint model with latent time shifts. This joint model uses a multivariate nonlinear mixed-effects model with latent time shifts to model the correlated longitudinal markers of cognitive decline, and simultaneously, a proportional hazards model to incorporate dropout due to dementia or death. We investigate two association structures between the longitudinal process and the time to event process: the current value structure and the shared random effect structure. We compare the proposed joint model with separate models that ignore informative dropout across various simulation settings. The proposed joint models with correctly specified association structures show the best performance. Even the models with misspecified association structures outperform the separate models that does not consider informative dropout. We conclude that our proposed joint model with latent time shifts offers more accurate and robust estimates than the latent time disease progression models that neglect informative dropout. Future research will involve incorporating competing risks and other parametrizations of the longitudinal model into this joint model framework.
Functional dissection of the conservedC. elegansLEM-3/ANKLE1 nuclease reveals a crucial requirement for the LEM-like and GIY-YIG domains for DNA bridges processing
Authors: Song Junfang , Geary Peter , Hong Ye , Rolland Stéphane G.M. , Garter Anton 
Published: June 2024

AbstractFaithful chromosome segregation requires the removal of all DNA bridges physically linking chromatids before the completion of cell division. While several redundant safeguard mechanisms to process these DNA bridges exist from S-phase to late anaphase, the conserved LEM-3/ANKLE1 nuclease has been proposed to be part of a ‘last chance’ mechanism that acts at the midbody to eliminate DNA bridges that persist until late cytokinesis. We show that LEM-3 can cleave a wide range of branched DNA substrates, including flaps, forks, nicked and intact Holliday Junctions. AlphaFold modeling data suggest that the catalytic mechanism of LEM-3/ANKLE1 is conserved, mirroring the mechanism observed in bacterial GIY-YIG nucleases. We also present evidence that LEM-3 may form a homodimeric complex on the Holliday Junction DNA. LEM-3 DNA binding capacity requires both the LEM-like and the GIY-YIG nuclease domains; both are also essential for LEM-3 recruitment to the midbody and its nuclease activity. Finally, we show that preventing LEM-3 nuclear access is important to avoid toxicity, likely caused by branched DNAs cleavage during normal DNA metabolism. Our data suggest thatC. elegansLEM-3 acts as a ‘last chance catch-all’ enzyme that processes DNA bridges caused by various perturbations of DNA metabolism just before cells divide.