bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuronal inclusions of α‐synuclein contribute to the pathogenesis of Krabbe disease
Authors: Smith Benjamin R , Santos Marta B , Marshall Michael S , Cantuti‐Castelvetri Ludovico , Lopez‐Rosas Aurora , Li Guannan , van Breemen Richard , Claycomb Kumiko I , Gallea Jose I , Celej Maria S , Crocker Stephen J , Givogri Maria I , Bongarzone Ernesto R 
Published: January 2014

AbstractDemyelination is a major contributor to the general decay of neural functions in children with Krabbe disease. However, recent reports have indicated a significant involvement of neurons and axons in the neuropathology of the disease. In this study, we have investigated the nature of cellular inclusions in the Krabbe brain. Brain samples from the twitcher mouse model for Krabbe disease and from patients affected with the infantile and late‐onset forms of the disease were examined for the presence of neuronal inclusions. Our experiments demonstrated the presence of cytoplasmic aggregates of thioflavin‐S‐reactive material in both human and murine mutant brains. Most of these inclusions were associated with neurons. A few inclusions were detected to be associated with microglia and none were associated with astrocytes or oligodendrocytes. Thioflavin‐S‐reactive inclusions increased in abundance, paralleling the development of neurological symptoms, and distributed throughout the twitcher brain in areas of major involvement in cognition and motor functions. Electron microscopy confirmed the presence of aggregates of stereotypic β‐sheet folded proteinaceous material. Immunochemical analyses identified the presence of aggregated forms of α‐synuclein and ubiquitin, proteins involved in the formation of Lewy bodies in Parkinson's disease and other neurodegenerative conditions. In vitro assays demonstrated that psychosine, the neurotoxic sphingolipid accumulated in Krabbe disease, accelerated the fibrillization of α‐synuclein. This study demonstrates the occurrence of neuronal deposits of fibrillized proteins including α‐synuclein, identifying Krabbe disease as a new α‐synucleinopathy. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd
Long‐Term Results After Adult Ex Situ Split Liver Transplantation Since Its Introduction in 1987
Authors: Schrem Harald , Kleine Moritz , Lankisch Tim Oliver , Kaltenborn Alexander , Kousoulas Lampros , Zachau Lea , Lehner Frank , Klempnauer Jürgen 
Published: January 2014

AbstractBackgroundSplit liver transplantation is still discussed controversially. Utilization of split liver grafts has been declining since a change of allocation rules for the second graft abolished incentives for German centres to perform ex situ splits. We therefore analysed our long‐term experiences with the first ex situ split liver transplant series worldwide.MethodsA total of 131 consecutive adult ex situ split liver transplants (01.12.1987–31.12.2010) were analysed retrospectively.ResultsThirty‐day mortality rates and 1‐ and 3‐year patient survival rates were 13, 76.3, and 66.4 %, respectively. One‐ and three‐year graft survival rates were 63.4 and 54.2 %, respectively. The observed 10‐year survival rate was 40.6 %. Continuous improvement of survival from era 1 to 3 was observed (each era: 8 years), indicating a learning curve over 24 years of experience. Patient and graft survival were not influenced by different combinations of transplanted segments or types of biliary reconstruction (p > 0.05; Cox regression). Patients transplanted for primary sclerosing cholangitis had better survival (p = 0.021; log‐rank), whereas all other indications including acute liver failure (13.6 %), acute and chronic graft failure (9.1 %) had no significant influence on survival (p > 0.05; log‐rank). Biliary complications (27.4 %) had no significant influence on patient or graft survival (p > 0.05; log‐rank). Hepatic artery thrombosis (13.2 %) had a significant influence on graft survival but not on patient survival (p = 0.002, >0.05, respectively; log‐rank).ConclusionsSplit liver transplantation can be used safely and appears to be an underutilized resource that may benefit from liberal allocation of the second graft.
Governance Processes and Change Within Organizational Participants of Multi‐sectoral Community Health Care Alliances: The Mediating Role of Vision, Mission, Strategy Agreement and Perceived Alliance Value
Authors: Hearld Larry R. , Alexander Jeffrey A. 
Published: January 2014

AbstractMulti‐sectoral community health care alliances are organizations that bring together individuals and organizations from different industry sectors to work collaboratively on improving the health and health care in local communities. Long‐term success and sustainability of alliances are dependent on their ability to galvanize participants to take action within their ‘home’ organizations and institutionalize the vision, goals, and programs within participating organizations and the broader community. The purpose of this study was to investigate two mechanisms by which alliance leadership and management processes may promote such changes within organizations participating in alliances. The findings of the study suggest that, despite modest levels of change undertaken by participating organizations, more positive perceptions of alliance leadership, decision making, and conflict management were associated with a greater likelihood of participating organizations making changes as a result of their participation in the alliance, in part by promoting greater vision, mission, and strategy agreement and higher levels of perceived value. Leadership processes had a stronger relationship with change within participating organizations than decision‐making style and conflict management processes. Open‐ended responses by participants indicated that participating organizations most often incorporated new measures or goals into their existing portfolio of strategic plans and activities in response to alliance participation.
Preparation and Physiochemical Properties of Disodium Lauryl Glucoside Sulfosuccinate
Authors: Gao Yuyang , Yang Xiuquan , Bai Liang , Zhang Jun 
Published: January 2014

AbstractA new synthetic route to a mild surfactant disodium lauryl glucoside sulfosuccinate (AG‐SS) containing two hydrophilic groups is described and its measured physicochemical properties reported. AG‐SS was synthesized from lauryl glucoside reacted with maleic acid anhydride, and then sulfonated with sodium sulfite. The structure and composition of the product were defined by Fourier transform infrared spectroscopy and liquid chromatography–mass spectrometry. The surface activity measurement showed that the critical micelle concentration (CMC) and that the surface tension at CMC (γCMC) of AG‐SS were 2.59 × 10−4 mol/L and 35.21 mN/m at 25 °C, respectively. AG‐SS exhibited excellent water solubility eliminating the disadvantage of lauryl glucoside; its foaming ability was also remarkable.
Development of anonymous nuclear markers from Illumina paired-end data for Seychelles caecilian amphibians (Gymnophiona: Indotyphlidae)
Authors: Lewis Claire J. , Maddock Simon T. , Day Julia J. , Nussbaum Ronald A. , Morel Charles , Wilkinson Mark , Foster Peter G. , Gower David J. 
Published: January 2014

Abstract
Anonymous nuclear markers were developed for Seychelles caecilian amphibians. Using a previously published bioinformatics pipeline (developed for Roche 454 data), 36 candidate anonymous nuclear loci (ANL) of at least 180 bp length were identified from Illumina MiSeq next generation sequencing data for five Seychelles species. We designed primer pairs for the 36 candidate ANL and tested these by PCR and Sanger sequencing. Seven ANL amplified and sequenced well for at least five of the six nominal Seychelles caecilian species (in three genera), and represent potentially useful markers for systematics and conservation.
Time course of brain volume changes in the preclinical phase of Alzheimer's disease
Authors: Bernard Charlotte , Helmer Catherine , Dilharreguy Bixente , Amieva Hélène , Auriacombe Sophie , Dartigues Jean‐François , Allard Michèle , Catheline Gwénaëlle 
Published: January 2014

AbstractBackgroundStructural alterations of a large network characterize Alzheimer's disease (AD), but the time course of these changes remains unclear. The dynamic of these alterations was examined in the AD preclinical phase using data from the 10‐year follow‐up of a population‐based cohort (Bordeaux‐3City).MethodsParticipants received neuropsychological assessments every 2 years and two identical magnetic resonance imaging (MRI) exams at baseline and 4 years later. Twenty‐five incident AD cases were compared with 319 subjects who remained free of dementia. Subjects were free of dementia at baseline and at follow‐up MRI. Incident AD occurred after these time points.ResultsAt baseline, incident AD already presented smaller volumes only in the left amygdalo‐hippocampal complex. Moreover, a higher annual rate of atrophy of the temporoparietal cortices was observed in future AD subjects during the following 4 years.ConclusionIncident AD cases present mediotemporal lesions up to 5 years before diagnosis. This neurodegenerative process seems to progressively reach the temporoparietal cortices in the AD preclinical phase.
OPTIMAL BONUS-MALUS SYSTEMS USING FINITE MIXTURE MODELS
Authors: Tzougas George , Vrontos Spyridon , Frangos Nicholas 
Published: January 2014

AbstractThis paper presents the design of optimal Bonus-Malus Systems using finite mixture models, extending the work of Lemaire (1995; Lemaire, J. (1995) Bonus-Malus Systems in Automobile Insurance. Norwell, MA: Kluwer) and Frangos and Vrontos (2001; Frangos, N. and Vrontos, S. (2001) Design of optimal bonus-malus systems with a frequency and a severity component on an individual basis in automobile insurance. ASTIN Bulletin, 31(1), 1–22). Specifically, for the frequency component we employ finite Poisson, Delaporte and Negative Binomial mixtures, while for the severity component we employ finite Exponential, Gamma, Weibull and Generalized Beta Type II mixtures, updating the posterior probability. We also consider the case of a finite Negative Binomial mixture and a finite Pareto mixture updating the posterior mean. The generalized Bonus-Malus Systems we propose, integrate risk classification and experience rating by taking into account both the a priori and a posteriori characteristics of each policyholder.
The influence of Reynolds number on the triple point trajectories at shock reflection off cylindrical surfaces
Authors: Kleine H. , Timofeev E. , Hakkaki-Fard A. , Skews B. 
Published: January 2014

AbstractIn the unsteady process of shock reflection off convexly curved surfaces, the Reynolds number can have an influence on the development of the irregular reflection pattern. Time-resolved visualizations of the reflection process and high-resolution numerical simulation are used in this investigation to quantify this influence, which manifests itself in a delayed growth of the shock pattern with decreasing Reynolds number. In order to conduct reliable and unambiguous measurements, the present study concentrates on observing the development of the established irregular reflection pattern rather than attempting to determine the transition point directly. It can be seen that the influence of the Reynolds number is highly nonlinear and that changes of two orders of magnitude or more are required to produce a reliably measurable difference in the triple point trajectories, which is considerably more than what has so far been reported in the literature. The results allow one to make inferences regarding the transition process and they help to clarify previously reported discrepancies between predicted and experimentally determined transition angles.
JOINS AND COVERS IN INVERSE SEMIGROUPS AND TIGHT -ALGEBRAS
Authors: DONSIG ALLAN P. , MILAN DAVID 
Published: January 2014

AbstractWe show Exel’s tight representation of an inverse semigroup can be described in terms of joins and covers in the natural partial order. Using this, we show that the ${C}^{\ast } $-algebra of a finitely aligned category of paths, developed by Spielberg, is the tight ${C}^{\ast } $-algebra of a natural inverse semigroup. This includes as a special case finitely aligned higher-rank graphs: that is, for such a higher-rank graph $\Lambda $, the tight ${C}^{\ast } $-algebra of the inverse semigroup associated to $\Lambda $ is the same as the ${C}^{\ast } $-algebra of $\Lambda $.
The intrinsic cost of cognitive control
Authors: Kool Wouter , Botvinick Matthew 
Published: January 2014

AbstractKurzban and colleagues carry forward an important contemporary movement in cognitive control research, tending away from resource-based models and toward a framework focusing on motivation or value. However, their specific proposal, centering on opportunity costs, appears problematic. We favor a simpler view, according to which the exertion of cognitive control carries intrinsic subjective costs.
Weather and livestock risk factors for Escherichia coli O157 human infection in Alberta, Canada
Authors: BIFOLCHI N. , MICHEL P. , TALBOT J. , SVENSON L. , SIMMONDS K. , CHECKLEY S. , CHUI L. , DICK P. , WILSON J. B. 
Published: January 2014

SUMMARYThis study investigated the extent to which proximity to cattle and weather events in Alberta predispose human populations to E. coli O157 disease. Cases of human E. coli O157 infection in Alberta between 2004 and 2011 were obtained from the province's Communicable Disease Reporting System and Discharge Abstract Database. Regression models based on spatial area incorporated human infection data with livestock and weather covariates. A variety of regression models were applied (i.e. least squares, spatial lag/error, Poisson, negative binomial) to test the most appropriate approach. Ratios for the total number of calves, bulls and beef cows to human population were highlighted as significant cattle density variables in all final best-fitting models. Weather variables were not significant in final regression models averaged over the full study period. Our results provide evidence of a significant association between measures of cattle density and human E. coli O157 disease in Alberta.
Is vitamin D status associated with open-angle glaucoma? A cross-sectional study from South Korea
Authors: Yoo Tae Keun , Oh Ein , Hong Samin 
Published: January 2014

AbstractObjectiveOpen-angle glaucoma (OAG) is one of the major chronic diseases involving the optic nerve. However, little is known about the association between vitamin D and OAG. The present study was conducted to test the hypothesis that lower vitamin D status is associated with greater prevalence of OAG.DesignCross-sectional study. Multivariable logistic regression was performed to examine the relationship between serum 25-hydroxyvitamin D (25(OH)D) and OAG after adjusting for traditional potential confounders. OAG was defined by the criteria of the International Society for Geographical and Epidemiological Ophthalmology.SettingThe Fifth Korean National Health and Nutrition Examination Survey conducted in 2010–2011.SubjectsSix thousand and ninety-four adult participants randomly selected from 192 surveys in 131 locations in South Korea.ResultsMultivariable-adjusted odds ratios of OAG across quintiles of decreasing 25(OH)D were 1·26, 1·00 (reference), 1·31, 1·36 and 1·69 (P for quadratic trend <0·01). The odds ratio for the lowest 25(OH)D quintile was significantly higher than that for the second quintile (P < 0·01). In addition, we discovered that the predictors for worsening of OAG, such as intraocular pressure or vertical and horizontal cup-to-disc ratios, had a significant relationship with 25(OH)D level.ConclusionsThere was a reverse J-shaped association between 25(OH)D levels and the risk of OAG, with significantly elevated risk at lower 25(OH)D. The findings of this research suggest that vitamin D deficiency should be considered as a potential risk factor for the development of OAG. To our knowledge, the present study is the first one that shows an association between vitamin D status and OAG.
Lack of common TCRA and TCRB clonotypes in CD8+/TCRαβ+ T-cell large granular lymphocyte leukemia: a review on the role of antigenic selection in the immunopathogenesis of CD8+ T-LGL
Authors: Sandberg Y , Kallemeijn M J , Dik W A , Tielemans D , Wolvers-Tettero I L M , van Gastel-Mol E J , Szczepanski T , Pol Y , Darzentas N , van Dongen J J M , Langerak A W 
Published: January 2014

AbstractClonal CD8+/T-cell receptor (TCR)αβ+ T-cell large granular lymphocyte (T-LGL) proliferations constitute the most common subtype of T-LGL leukemia. Although the etiology of T-LGL leukemia is largely unknown, it has been hypothesized that chronic antigenic stimulation contributes to the pathogenesis of this disorder. In the present study, we explored the association between expanded TCR-Vβ and TCR-Vα clonotypes in a cohort of 26 CD8+/TCRαβ+ T-LGL leukemia patients, in conjunction with the HLA-ABC genotype, to find indications for common antigenic stimuli. In addition, we applied purpose-built sophisticated computational tools for an in-depth evaluation of clustering of TCRβ (TCRB) complementarity determining region 3 (CDR3) amino-acid LGL clonotypes. We observed a lack of clear TCRA and TCRB CDR3 homology in CD8+/TCRαβ+ T-LGL, with only low level similarity between small numbers of cases. This is in strong contrast to the homology that is seen in CD4+/TCRαβ+ T-LGL and TCRγδ+ T-LGL and thus underlines the idea that the LGL types have different etiopathogenesis. The heterogeneity of clonal CD8+/TCRαβ+ T-LGL proliferations might in fact suggest that multiple pathogens or autoantigens are involved.
Exogenous rhTRX reduces lipid accumulation under LPS-induced inflammation
Authors: Han Gi-Yeon , Lee Eun-Kyung , Park Hey-won , Kim Hyun-Jung , Kim Chan-Wha 
Published: January 2014

AbstractRedox-regulating molecule, recombinant human thioredoxin (rhTRX) which shows anti-inflammatory, and anti-oxidative effects against lipopolysaccharide (LPS)-stimulated inflammation and regulate protein expression levels. LPS-induced reactive oxygen intermediates (ROI) and NO production were inhibited by exogenous rhTRX. We identified up/downregulated intracellular proteins under the LPS-treated condition in exogenous rhTRX-treated A375 cells compared with non-LPS-treated cells via 2-DE proteomic analysis. Also, we quantitatively measured cytokines of in vivo mouse inflammation models using cytometry bead array. Exogenous rhTRX inhibited LPS-stimulated production of ROI and NO levels. TIP47 and ATP synthase may influence the inflammation-related lipid accumulation by affecting lipid metabolism. The modulation of skin redox environments during inflammation is most likely to prevent alterations in lipid metabolism through upregulation of TIP47 and ATP synthase and downregulation of inflammatory cytokines. Our results demonstrate that exogenous rhTRX has anti-inflammatory properties and intracellular regulatory activity in vivo and in vitro. Monitoring of LPS-stimulated pro-inflammatory conditions treated with rhTRX in A375 cells could be useful for diagnosis and follow-up of inflammation reduction related with candidate proteins. These results have a therapeutic role in skin inflammation therapy.
Parasitism, condition and reproduction of the European hake (Merluccius merluccius) in the northwestern Mediterranean Sea
Authors: Ferrer-Maza Dolors , Lloret Josep , Muñoz Marta , Faliex Elisabeth , Vila Sílvia , Sasal Pierre 
Published: January 2014

AbstractIt is well known that parameters relating to physical condition and reproduction of fish provide essential data for estimating the productivity of exploited populations, as is the case with the European hake (Merluccius merluccius) in the northwestern Mediterranean Sea. Although parasitism might affect these parameters, research in this area is very scarce (and in the case of the Mediterranean almost non-existent). This study evaluates for the first time the potential link between parasitism, condition and reproduction of the European hake. Indicators of fish energy reserves (total lipid content in liver and gonads) and reproductive capacity (fecundity, egg quality and atresia) were evaluated, as were the prevalence and intensity of infection by metazoan parasites. The results indicate that the impact of anisakid nematodes is mostly negative and occurs mainly when hake are allocating their energy reserves to gonadal development. Although the results reveal a link between parasitism, condition and reproduction, we concluded that the northwestern Mediterranean hake population is in equilibrium with its metazoan parasites, which are not causing severe impairment to their physical condition or reproductive capacity.
Opening Injection Pressure Consistently Detects Needle–Nerve Contact during Ultrasound-guided Interscalene Brachial Plexus Block
Authors: Gadsden Jeff C. , Choi Jason J. , Lin Emily , Robinson Allegra 
Published: January 2014

Abstract

Background:
Needle trauma may cause neuropathy after nerve blockade. Even without injection, nerve injury can result from forceful needle–nerve contact (NNC). High opening injection pressures (OIPs) have been associated with intrafascicular needle tip placement and nerve damage; however, the relationship between OIP and NNC is unclear. The authors conducted a prospective, observational study to define this relationship.

Methods:
Sixteen patients scheduled for shoulder surgery under interscalene block were enrolled if they had clear ultrasound images of the brachial plexus roots. A 22-gauge stimulating needle was inserted within 1 mm of the root, and 1-ml D5W injected at 10 ml/min by using an automated pump. OIP was monitored using an in-line pressure manometer and injections aborted if 15 psi or greater. The needle was advanced to displace the nerve slightly (NNC), and the procedure repeated. Occurrence of evoked motor response and paresthesia were recorded.

Results:
Fifteen patients had at least one clearly visible root. OIP at 1 mm distance from the nerve was less than 15 psi (mean peak pressure 8.2 ± 2.4 psi) and the 1-ml injection could be completed in all but two cases (3%). In contrast, OIP during NNC was 15 psi or greater (mean peak pressure 20.9 ± 3.7 psi) in 35 of 36 injections. Aborting the injection when OIP reached 15 psi prevented commencement of injection in all cases of NNC except one.

Conclusion:
High OIP (≥15 psi) consistently detected NNC, suggesting that injection pressure monitoring may be useful in preventing injection against nerve roots during interscalene block.