bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prognostic value of diagnosing concurrent multiple myeloma in immunoglobulin light chain amyloidosis
Authors: Dinner Shira , Witteles Wesley , Witteles Ronald , Lam Anthony , Arai Sally , Lafayette Richard , George Tracy I. , Schrier Stanley L. , Liedtke Michaela 
Published: February 2013

SummaryThe prevalence and prognostic value of a concomitant diagnosis of symptomatic or asymptomatic multiple myeloma (MM), as defined by the current International Myeloma Working Group (IMWG) criteria, in patients with immunoglobulin light chain amyloidosis (AL), are unknown. We studied 46 consecutive patients with AL who underwent quantification of serum M‐protein and clonal bone marrow plasma cells, as well as a comprehensive evaluation for end organ damage by MM. Using standard morphology and CD138 immunohistochemical staining, 57% and 80% of patients were found to have concomitant MM, respectively. Nine patients exhibited end organ damage consistent with a diagnosis of symptomatic MM. While overall survival was similar between AL patients with or without concurrent myeloma (1‐year overall survival 68% vs. 87%; P = 0·27), a diagnosis of symptomatic myeloma was associated with inferior outcome (1‐year overall survival 39% vs. 81%; P = 0·005). Quantification of bone marrow plasma cells by both standard morphology and CD138 immunohistochemistry identified a much higher prevalence of concurrent MM in patients with AL than previously reported. Evaluation of bone marrow plasma cell infiltration and presence of myeloma associated end organ damage could be clinically useful for prognostication of patients with AL.
Conversational flow and entitativity: The role of status
Authors: Koudenburg Namkje , Postmes Tom , Gordijn Ernestine H. 
Published: February 2013

This study examines the process by which perceptions of conversational flow foster an emergent sense of group entitativity. We propose that conversational flow influences more than just the quality of interpersonal relations: it signals entitativity – social unity at the group level. We predicted that when conversations are intermitted by brief silences after a target has spoken, this is perceived as disruptive for targets of low social status within the group: For low‐status group members, such pauses raise concerns over respect and inclusion. However, for high‐status group members, a similar intermission may be interpreted as an acknowledgement of their distinctive position in the group, and may therefore bolster the hierarchy and unity of the group. Two experiments support these hypotheses. Study 1 (N = 77) manipulated status in conversations of a target participant with confederates. Study 2 (N = 138) replicates the effect among participants who watch a videotaped conversation. Both studies show the predicted pattern, and suggest that belonging (Study 1) and perceived respect (Study 2) may mediate effects of condition on perceptions of group entitativity.
Psychosocial risk factors, pre‐motor symptoms and first‐time hospitalization with Parkinson's disease: a prospective cohort study
Authors: Clark A. J. , Ritz B. , Prescott E. , Rod N. H. 
Published: February 2013

Background and purposeExperimental studies support a link between stress and development of parkinsonian symptoms, but prospective population studies are lacking. The aim of the current study is to determine the effects of several psychosocial factors on the risk of Parkinson's disease (PD), as well as to identify potential pre‐motor symptoms for PD in a large prospective cohort study.MethodsIn 1991–1993, a total of 9955 women and men free of PD from the Copenhagen City Heart Study were asked about major life events, economic hardship, social network, impaired sleep and vital exhaustion. The participants were followed for first‐time hospitalization with PD in nationwide registers until 2011.ResultsVital exhaustion was associated with a higher risk of PD hospitalization in an exposure‐dependent manner (Ptrend = 0.001), with high vs. low vital exhaustion being associated with a hazard ratio of 2.50 [95% confidence interval (CI): 1.28–4.89]. A slightly higher risk of PD hospitalization (hazard ratio = 1.49; 95% CI: 0.87–2.56) was suggested in participants with impaired sleep at baseline. No more than weak associations were observed for economic hardship, major life events or inadequate social network in the current study.ConclusionsOverall, the hypothesis that psychosocial risk factors affect the risk of PD is not supported. The results, however, suggest that vital exhaustion may be a pre‐motor marker of the neurodegenerative process eventually leading to motor symptoms and clinical PD. Vital exhaustion may be useful for screening aimed at early detection and when considering disease‐modifying therapies in people at high risk of clinical PD.
Does inhibition of proteolytic activity improve adhesive luting?
Authors: Lührs Anne‐Katrin , De Munck Jan , Geurtsen Werner , Van Meerbeek Bart 
Published: February 2013

Endogenous enzymes may be involved in the biodegradation of adhesive restoration–tooth interfaces. Inhibitors of matrix metalloproteinases (MMPs) have been suggested to retard the bond‐degradation process. Limited data are available on whether composite cements may also benefit from MMP inhibitors. Therefore, the aim of this study was to determine the effect of two MMP inhibitors – chlorhexidine digluconate (CHX) and galardin – on the microtensile bond strength (μTBS) of two self‐adhesive composite cements to dentin. Ceramic specimens were cemented to bur‐cut dentin surfaces using the self‐adhesive composite cements RelyX Unicem 2 (3M ESPE) or Clearfil SA (Kuraray), or the etch‐and‐rinse composite cement Nexus 3 (Kerr) that served as the control. The surfaces were left untreated or were pretreated with MMP inhibitors (2% CHX or 0.2 mM galardin). The μTBS was determined ‘immediately’ and upon ageing (water storage for 6 months). Statistical analysis revealed a significant effect of the factors ‘composite cement’ and ‘storage’, as well as all interactions, but no effect of the MMP inhibitors. After 6 months of ageing, the μTBS decreased for all cements, except for the multistep etch‐and‐rinse luting composite when it was applied without MMP inhibitors. The MMP inhibitors could not prevent the decrease in μTBS upon ageing and therefore do not improve the luting durability of the composite cements tested.
Susceptibility of Intact Germinating Arabidopsis thaliana to Human Fungal Pathogens Cryptococcus neoformans and C. gattii
Authors: Warpeha Katherine M. , Park Yoon-Dong , Williamson Peter R. 
Published: February 2013

ABSTRACT

The fungus
Cryptococcus
contributes a large global burden of infectious death in both HIV-infected and healthy individuals. As
Cryptococcus
is an opportunistic pathogen, much of the evolutionary pressure shaping virulence occurs in environments in contact with plants and soil. The present studies investigated inoculation of intact seeds of the common weed
Arabidopsis thaliana
with fungal cells over a 21-day period.
C. gattii
was the more virulent plant pathogen, resulting in disrupted germination as well as increased stem lodging, fungal burden, and plant tissue colocalization.
C. neoformans
was a less virulent plant pathogen but exhibited prolonged tissue residence within the cuticle and vascular spaces. Arabidopsis mutants of the
PRN1
gene, which is involved in abiotic and biotic signaling affecting phenylalanine-derived flavonoids, showed altered susceptibility to cryptoccocal infections, suggesting roles for this pathway in cryptococcal defense. The fungal virulence factor laccase was also implicated in plant pathogenesis, as a cryptococcal
lac1
Δ strain was less virulent than wild-type fungi and was unable to colonize seedlings. In conclusion, these studies expand knowledge concerning the ecological niche of
Cryptococcus
by demonstrating the pathogenic capacity of the anamorphic form of cryptococcal cells against healthy seedlings under physiologically relevant conditions. In addition, an important role of laccase in plant as well as human virulence may suggest mechanisms for laccase retention and optimization during evolution of this fungal pathogen.
Behavior of Escherichia coli in a Heterogeneous Gelatin-Dextran Mixture
Authors: Boons K. , Mertens L. , Van Derlinden E. , David C. C. , Hofkens J. , Van Impe J. F. 
Published: February 2013

ABSTRACT

In a gelatin-dextran mixture, changing the (relative and/or absolute) concentration of the components leads to the formation of different microstructures. Confocal laser scanning microscopy illustrated that the nature of the microstructure determines the location and morphology of
Escherichia coli
colonies. Observations indicate that bacterial growth preferentially occurs in the dextran phase, regardless of the microstructure.
Midazolam for urethral catheterisation in female infants with suspected urinary tract infection: a case-control study
Authors: Shavit Itai , Feraru Liat , Miron Dan , Weiser Giora 
Published: February 2013

ObjectivesBased on the 2010 Israeli Medical Association recommendations, young children with suspected urinary tract infection (UTI) are mildly sedated with oral or intranasal midazolam to reduce the distress associated with urethral catheterisation (UC). The primary objective of this study was to examine the rate of urine culture contamination (UCC) in infants who underwent UC with and without sedation. Other objectives were to evaluate serious adverse events and emergency department (ED) length of stay.MethodsA retrospective case-control study was conducted in a paediatric ED.ResultsTwo cohorts of patients who underwent UC were compared, 164 female infants who were sedated with midazolam (case subjects) and 173 who were not (controls). Cases and controls had a mean temperature of 38.3°C and 38.2°C, respectively. One hundred and forty-one patients were treated with oral midazolam and 23 received the drug intranasally. Cases and controls had a UCC rate of 20/164 (12%) and 45/173 (26%), respectively. Compared with controls, cases had lower odds of UCC (OR=0.39, 95% CI 0.21 to 0.73).Serious adverse events related to midazolam were not recorded. Case subjects and controls had a mean ED length of stay of 2.96 h and 2.50 h, respectively. The difference between the groups was statistically significant (p<0.014, 95% CI 0.10 to 0.90 for difference between means).ConclusionsIn this cohort of febrile infants, sedation with oral or intranasal midazolam reduced the risk of culture contamination during UC without causing serious adverse events. However, patients who were treated with sedation had longer length of stay in the ED.
The Combination of Sorafenib and Everolimus Abrogates mTORC1 and mTORC2 Upregulation in Osteosarcoma Preclinical Models
Authors: Pignochino Ymera , Dell'Aglio Carmine , Basiricò Marco , Capozzi Federica , Soster Marco , Marchiò Serena , Bruno Stefania , Gammaitoni Loretta , Sangiolo Dario , Torchiaro Erica , D'Ambrosio Lorenzo , Fagioli Franca , Ferrari Stefano , Alberghini Marco , Picci Piero , Aglietta Massimo , Grignani Giovanni 
Published: February 2013

Abstract
Purpose: The multikinase inhibitor sorafenib displays antitumor activity in preclinical models of osteosarcoma. However, in sorafenib-treated patients with metastatic-relapsed osteosarcoma, disease stabilization and tumor shrinkage were short-lived and drug resistance occurred. We explored the sorafenib treatment escape mechanisms to overcome their drawbacks.
Experimental Design: Immunoprecipitation, Western blotting, and immunohistochemistry were used to analyze the mTOR pathway [mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2)]. Cell viability, colony growth, and cell migration were evaluated in different osteosarcoma cell lines (MNNG-HOS, HOS, KHOS/NP, MG63, U-2OS, SJSA-1, and SAOS-2) after scalar dose treatment with sorafenib (10–0.625 μmol/L), rapamycin-analog everolimus (100–6.25 nmol/L), and combinations of the two. Cell cycle, reactive oxygen species (ROS) production, and apoptosis were assessed by flow cytometry. Nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice injected with MNNG-HOS cells were used to determine antitumor and antimetastatic effects. Angiogenesis and vascularization were evaluated in vitro by exploiting endothelial branching morphogenesis assays and in vivo in xenografted mice and chorioallantoic membranes.
Results: After sorafenib treatment, mTORC1 signaling was reduced (downstream target P-S6), whereas mTORC2 was increased (phospho-mTOR Ser2481) in MNNG-HOS xenografts compared with vehicle-treated mice. Combining sorafenib with everolimus resulted in complete abrogation of both mTORC1 [through ROS-mediated AMP-activated kinase (AMPK) activation] and mTORC2 (through complex disassembly). The sorafenib/everolimus combination yielded: (i) enhanced antiproliferative and proapoptotic effects, (ii) impaired tumor growth, (iii) potentiated antiangiogenesis, and (iv) reduced migratory and metastatic potential.
Conclusion: mTORC2 activation is an escape mechanism from sorafenib treatment. When sorafenib is combined with everolimus, its antitumor activity is increased by complete inhibition of the mTOR pathway in the preclinical setting. Clin Cancer Res; 19(8); 2117–31. ©2013 AACR.
A Meta-Analysis of the Dimensions of a Learning Organization Questionnaire
Authors: Watkins Karen E. , Dirani Khalil M. 
Published: February 2013

The Problem Organizations strive to develop their employees to a level that meets current and future needs at a time when those needs are great and resources scarce. Human resource developers are charged with finding solutions; yet many of the changes essential to doing this are in the culture, and, senior leaders have greater impact on the culture. Effective ways to alert senior individuals to the cultural imperatives are needed. Unfortunately, human resource and organization development (HROD) scholars have had few valid organizational measures with which to demonstrate the status of learning and the impact of learning on the organization. In 2003, we shared the Dimensions of a Learning Organization Questionnaire (DLOQ) in this journal. It behooves us to examine what has occurred using this instrument since that time. The Solution One approach is to build a self-sustaining learning architecture that is so embedded in the culture and even the work itself that it evolves and grows with the organization. A valid and reliable diagnostic enables HROD practitioners to assess where the learning culture is at the time of its administration, and signals organization members to critical changes needed to develop a more effective learning infrastructure. Armed with this information, they can make the business case and guide proposed interventions. Scholars can demonstrate key relationships between a learning culture and knowledge, financial, and mission performance. This article and others in this special issue examine what has been learned in the last decade using the DLOQ. The Stakeholders HROD developers and senior leaders are significant stakeholders for this information. In addition, HROD scholars who hope to document the nature of the learning culture in a variety of contexts and to demonstrate the importance of having a strong learning culture to valued organizational outcomes will find a resource in the DLOQ.
TP53, SF3B1, and NOTCH1 mutations and outcome of allotransplantation for chronic lymphocytic leukemia: six-year follow-up of the GCLLSG CLL3X trial
Authors: Dreger Peter , Schnaiter Andrea , Zenz Thorsten , Böttcher Sebastian , Rossi Marianna , Paschka Peter , Bühler Andreas , Dietrich Sascha , Busch Raymonde , Ritgen Matthias , Bunjes Donald , Zeis Matthias , Stadler Michael , Uharek Lutz , Scheid Christof , Hegenbart Ute , Hallek Michael , Kneba Michael , Schmitz Norbert , Döhner Hartmut , Stilgenbauer Stephan 
Published: February 2013

Key Points
This trial update shows that allotransplantation can provide long-term minimal residual disease–negative disease control in poor-risk chronic lymphocytic leukemia. Six-year survival is close to 60% and is independent of the presence of TP53, SF3B1, and NOTCH1 mutations in the tumor clone.
Changes of bacterioplankton apparent species richness in two ornamental fish aquaria
Authors: Vlahos Nikolaos , Kormas Konstantinos Ar , Pachiadaki Maria G , Meziti Alexandra , Hotos George N , Mente Eleni 
Published: February 2013

Abstract
We analysed the 16S rRNA gene diversity within the bacterioplankton community in the water column of the ornamental fish Pterophyllum scalare and Archocentrus nigrofasciatus aquaria during a 60-day growth experiment in order to detect any dominant bacterial species and their possible association with the rearing organisms. The basic physical and chemical parameters remained stable but the bacterial community at 0, 30 and 60 days showed marked differences in bacterial cell abundance and diversity. We found high species richness but no dominant phylotypes were detected. Only few of the phylotypes were found in more than one time point per treatment and always with low relative abundance. The majority of the common phylotypes belonged to the Proteobacteria phylum and were closely related to Acinetobacter junii, Pseudomonas sp., Nevskia ramosa, Vogesella perlucida, Chitinomonas taiwanensis, Acidovorax sp., Pelomonas saccharophila and the rest belonged to the α-Proteobacteria, Bacteroidetes, Actinobacteria, candidate division OP11 and one unaffiliated group. Several of these phylotypes were closely related to known taxa including Sphingopyxis chilensis, Flexibacter aurantiacus subsp. excathedrus and Mycobacterium sp. Despite the high phylogenetic diversity most of the inferred ecophysiological roles of the found phylotypes are related to nitrogen metabolism, a key process for fish aquaria.
Remarkable Enhancement of Sensitivity with the Second Generation of Elliptically Polarization-detected Circular Dichroism Spectroscopy
Authors: Murakami Makoto , Araki Yasuyuki , Sakamoto Seiji , Hamada Yoshiki , Wada Takehiko 
Published: February 2013

Abstract
A new circular dichroism (CD) measurement system based on the detection of elliptically polarized light has been proposed. The approach has several unique and inherent advantages, such as sensitivities, reliability, and reproducibility, over conventional systems and Kliger’s pioneering work. We have rederived the Jones calculus and introduced the angular term into the formulation revealing that this approach should improve the signal intensity by controlling the light ellipticity. Therefore, we have first demonstrated the hypothesis by the quantitative analysis of the signal intensity measuring of the split CD pattern of tris(bipyridine)ruthenium(II) dichloride with the system. The CD signals increasing with decreasing the azimuth of retarder was clearly observed, enabling detection of CD signal with high sensitivity.
A Bidentate Ligand Immobilized on Silica via a Tripodal Linker Unit: Preparation and Application to Suzuki–Miyaura Coupling
Authors: Onozawa Shun-ya , Miyaji Takayuki , Fukaya Norihisa , Yoshinaga Michiyo , Ueda Masae , Takagi Yukio , Yasuda Hiroyuki 
Published: February 2013

Abstract
A new tripodal linker unit was synthesized and is expected to enable the robust immobilization of bidentate ligands such as 1,3-bis(diphenylphosphino)propane (DPPP) onto silica supports. It was subsequently used to anchor a palladium–DPPP complex onto ordered mesoporous silica, and the resulting catalyst showed moderate activity for the Suzuki–Miyaura coupling reaction of aryl chloride with phenylboronic acid. The catalyst was recycled three times without a significant loss in activity and with minimal leaching of palladium after the reaction.
From Weft and Warp of Weaving to Churning of Grinder: A Diasporic Trajectory in Amitav Ghoshs The Circle of Reason
Authors: Kulsum Umme 
Published: February 2013

Amitav Ghoshs first novel The Circle of Reason (1986) demonstrates the relationship between different discourses of knowledge (Science, History, Anthropology) and its colonial application to specific localities that authenticates key strategic policy to determine world trade and economy. In particular, the novel reflects how the emergence of science and textile mills shifts the paradigm of weaving world into the rubric of construction business. This paper focuses on how this manipulative change in world trade and economy predetermines the influx of people from one area to another with their existing skill (weaving) and profession. This paper examines three multicultural cities of three different localities that represent diasporic lives with all their struggle, potentiality, hybridity, mimicry and ambivalence. It will also highlight on how border-business, like trafficking, smuggling, is another important issue that creates newer trajectories for more profitable business (oil, bomb, gold, hashish) that encourages displacement of people. The character of Balaram, one of the major characters of part one of the novel, will be explained as a mimic character as he represents the colonial application of science in this subcontinent. Balaram has been presented according to Homi K. Bhabhas Of Mimicry and Man: The ambivalence of colonial discourse (121). And the diasporic cities like Lalpukur, al-Ghazira and El Oued can be 187 explained from Homi K. Bhabhas, Cultural Diversities and Cultural Differences. Stamford Journal of English; Volume 6; Page 186-202 DOI: http://dx.doi.org/10.3329/sje.v6i0.13913
Regulation of Cystic Fibrosis Transmembrane Conductance Regulator by MicroRNA-145, -223, and -494 Is Altered in ΔF508 Cystic Fibrosis Airway Epithelium
Authors: Oglesby Irene K. , Chotirmall Sanjay H. , McElvaney Noel G. , Greene Catherine M. 
Published: February 2013

Abstract
Expression of the cystic fibrosis transmembrane conductance regulator (CFTR) is altered in individuals with the ΔF508 CFTR mutation. We previously reported differential expression of microRNA (miRNA) in CF airway epithelium; however, the role of miRNA in regulation of CFTR expression here remains unexplored. In this study, we investigated the role of upregulated miRNAs in CFTR regulation in vivo in bronchial brushings from individuals homozygous or heterozygous for ΔF508 CFTR, validated our observations in vitro, and assessed the impact of defective chloride ion conductance, genotype, and colonization status on miRNA expression. miRNA target prediction was performed in silico, and expression of miRNA and target genes were measured by quantitative real-time PCR and/or Western blotting. Overexpression and inhibition studies were performed with pre-miRs or antimiRs, respectively, and a luciferase reporter gene was used to elucidate direct miRNA–target interactions. miR-145, miR-223, and miR-494 were upregulated in CF versus non-CF bronchial brushings and cell lines; in ΔF508 CFTR homozygotes versus heterozygotes; in subjects positive for P. aeruginosa; and in cells treated with a CFTR inhibitor or IL-1β. Reciprocal downregulation or upregulation of CFTR gene and/or protein expression was observed after miRNA manipulation and direct miRNA–target relationships demonstrated via a reporter system containing a wild type or mutated full-length CFTR 3′ untranslated region. Increased expression of miR-145, miR-223, and miR-494 in vivo in bronchial epithelium of individuals carrying the ΔF508 CFTR mutation correlates with decreased CFTR expression. Defective CFTR function, Pseudomonas colonization, and inflammation may affect miRNA expression and contribute to the regulation of ΔF508 CFTR.