bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of Cupferron‐lead(II) Complex for the Electrochemical Determination of dsDNA
Authors: Hui Ni , Wang Jia‐Sheng , Sun Wei 
Published: October 2013

AbstractBased on the interaction of cupferron and lead(II) complex [Cup‐Pb(II)] with double‐stranded DNA (dsDNA) a new voltammetric method for the detection of DNA was described in this paper. In pH 4.0 HAc‐hexamine buffer solution, [Cup‐Pb(II)] complex showed a sensitive second order derivative polarographic reductive peak at ‐0.554 V (vs. SCE). After the addition of dsDNA into [Cup‐Pb(II)] mixture solution the reductive peak current decreased with the positive shift of reductive peak potential, which was the typical characteristic of intercalation mode. Under the optimum conditions, the decrease of reductive peak current was directly proportional to the dsDNA concentration in the range from 1.0 to 25.0 mg/L with the linear regression equation as ΔIp″ (nA) = 129.30 + 62.51 C (mg/L) (n = 13, γ = 0.991). The detection limit of 0.90 mg/L (3σ) and the relative standard derivation (RSD) of 2.43% for 10 parallel determinations of 10.0 mg/L dsDNA were found. The method was successfully applied to synthetic samples with good results, and the stoichiometry of dsDNA with [Cup‐Pb(II)] complex was calculated by the voltammetic data with the binding number as 2 and the binding constant as 2.82 × 109.
One‐pot Synthesis of Tetrahydrobenzo[b]pyrans Catalyzed by PEG‐1000 Bridged Primary Amine Functionalized Dicationic Ionic Liquid in Water
Authors: Wang Ying‐Lei , Li Zhuo , Luo Jun , Liu Zu‐Liang 
Published: October 2013

AbstractA novel poly (ethylene glycol) bridged primary amine functionalized dicationic ionic liquid ([PA‐PEG1000‐DIL][BF4]) was synthesized and characterized by 1H‐NMR, 13C‐NMR, FT‐IR and ESI‐MS. The thermal gravimetric analysis (TGA) and differential scanning calorimetry (DSC) analysis indicated the high thermal stability of [PA‐PEG1000‐DIL][BF4]. It was used as an efficient and recyclable catalyst for the synthesis of substituted tetrahydrobenzo[b]pyrans through a one‐pot three‐component condensation of aromatic aldehydes, malononitrile and dimedone in 86% ∼ 96% yields within 10 ∼ 30 min in water. This method offers several advantages such as mild reaction conditions, simple operation and environmental friendliness. Furthermore, the catalyst could be easily recovered and reused for at least five runs without obvious loss of catalytic activity.
The Synthesis and Characterizations of Calcium Phosphate Nanoparticles via β‐Cyclodextrin, Poly(oxyethylene)5 Nonyl Phenol Ether and Cyclohexane Medium
Authors: Viswanathan Kaliyaperumal , Lee Y. C. , Fang Y. S. 
Published: October 2013

AbstractIn this work, the calcium phosphate nanoparticles have been produced by new reverse micro emulsion method containing β‐cyclodextrin, poly(oxyethylene)5 nonyl phenol ether and cyclohexane. Scanning electron microscope, transmission electron microscope, fourier transform infrared spectroscope and X‐ray diffraction were used to characterize the particles. The sizes of the nanoparticles were identified between 70‐80 nm. In conclusion, these results suggested that the developed reverse micro emulsion system based nanoparticles seem to be a promising formulation for calcium phosphate nanoparticles synthesis and it has immense potential in delivery of drugs and vaccines.
Theory and Applications of Sum‐Frequency Generations
Authors: Lin C. K. , Yang L. , Hayashi M. , Zhu C. Y. , Fujimura Y. , Shen Y. R. , Lin S. H. 
Published: October 2013

AbstractThe SFG technique has become popular since the pioneering work conducted by Shen's group in late 1980s. Combined the advantages of other surface‐detecting techniques, the SFG technique could identify both chemical species and orientations adsorbed on surfaces or inserted between surfaces. In view of the recent tremendous experimental activities of SFG in surface chemistry, biochemistry and material sciences, quantum chemical calculations of SFG spectra to analyze the experimental SFG spectra are badly needed. In this paper we presented the theoretical expressions of various SFG techniques and showed how to employ the quantum chemical calculations to simulate the corresponding SFG spectra.
Shying Away From a Good Thing: Social Anxiety in Romantic Relationships
Authors: Porter Eliora , Chambless Dianne L. 
Published: October 2013

ObjectivesTo determine whether social anxiety covaries with satisfaction, social support, and intimacy in romantic relationships.MethodUndergraduates and their romantic partners (N = 163) completed self‐report questionnaires.ResultsHigher social anxiety in women, but not men, was associated with wanting, receiving, and providing less support, based on self‐ but not partner‐report measures. Women higher in social anxiety also reported being less satisfied in their romantic relationships and self‐disclosing less to romantic partners than women lower in social anxiety. Further, self‐reported received support mediated the relationship between social anxiety and romantic relationship satisfaction in women. In both sexes, higher social anxiety was related to perceiving intimacy as riskier and romantic relationships as less emotionally intimate.ConclusionsTogether, results suggest that social anxiety is associated with interpersonal difficulty even in established romantic relationships.
A Multifaith Spiritually Based Intervention Versus Supportive Therapy for Generalized Anxiety Disorder: A Pilot Randomized Controlled Trial
Authors: Koszycki Diana , Bilodeau Cynthia , Raab‐Mayo Kelley , Bradwejn Jacques 
Published: October 2013

ObjectivesWe have previously reported that a multifaith spiritually based intervention (SBI) may have efficacy in the treatment of generalized anxiety disorder (GAD). This randomized pilot trial tested whether the SBI had greater efficacy than a nonspecific control condition in GAD.MethodTwenty‐three participants with GAD of at least moderate severity were randomized to 12 individual sessions of the SBI (n = 11) or supportive psychotherapy (SP)—our control condition (n = 12).ResultsIntent‐to‐treat analysis revealed the SBI fared better than SP in decreasing blind clinician ratings of anxiety and illness severity and self‐report worry and intolerance of uncertainty, with large between‐group effect sizes. The SBI also produced greater changes in spiritual well‐being. Results remained the same when supplementary analyses were performed on the completer sample. Treatment gains were maintained at 3‐months follow‐up.ConclusionsThis small pilot trial demonstrates that a nondenominational SBI has greater efficacy than a rigorous control in improving symptoms of GAD and enhancing spiritual well‐being. These results are encouraging and further research on the efficacy of the SBI and its underlying mechanisms is warranted.
Angiogenin Stimulates Ribosomal RNA Transcription by Epigenetic Activation of the Ribosomal DNA Promoter
Authors: Sheng Jinghao , Yu Wenhao , Gao Xiangwei , Xu Zhengping , Hu Guo‐Fu 
Published: October 2013

AbstractAngiogenin (ANG) undergoes nuclear translocation and promotes ribosomal RNA (rRNA) transcription thereby enhancing cell growth and proliferation. However, the mode of action of ANG in stimulating rRNA transcription is unclear. Here, we show that ANG enhances the formation of RNA polymerase I (Pol I) pre‐initiation complex at the ribosomal DNA (rDNA) promoter. ANG binds at the upstream control element (UCE) of the promoter and enhances promoter occupancy of RNA Pol I as well as the selectivity factor SL1 components TAFI48 and TAFI110. We also show that ANG increases the number of actively transcribing rDNA by epigenetic activation through promoter methylation and histone modification. ANG binds to histone H3, inhibits H3K9 methylation, and activates H3K4 methylation as well as H4 acetylation at the rDNA promoter. These data suggest that one of the mechanisms by which ANG stimulates rRNA transcription is through an epigenetic activation of rDNA promoter. J. Cell. Physiol. 229: 521–529, 2014. © 2013 Wiley Periodicals, Inc.
In Vivo Deletion of CAR Resulted in High Bone Mass Phenotypes in Male Mice
Authors: Cho Hwa Young , Jung Ju‐Yeon , Park Hyojung , Yang Jae‐Yeon , Jung Solip , An Jee Hyun , Cho Sun Wook , Kim Sang Wan , Kim Seong Yeon , Kim Jung Eun , Park Young Joo , Shin Chan Soo 
Published: October 2013

AbstractConstitutive androstane receptor (CAR) was originally identified as xenobiotic sensor that regulates the expression of cytochrome P450 genes. However, recent studies suggest that this nuclear receptor is also involved in the regulation of energy metabolism including glucose and lipid homeostasis. This study investigated the role of CAR in the regulation of bone mass in vivo using CAR−/− mice. Endogenous mRNA expression of CAR was observed in both primary osteoblasts and osteoclast precursors. CAR−/− mice have exhibited significant increase in whole body bone mineral density (BMD) by 9.5% (P < 0.01) and 5.5% (P < 0.05) at 10 and 15 weeks of age, respectively, compared with WT mice in males. Microcomputed tomography analysis of proximal tibia demonstrated a significant increase in trabecular bone volume (62.7%), trabecular number (54.1%) in male CAR−/− mice compared with WT mice. However, primary culture of calvarial cells exhibited no significant changes in osteogenic differentiation potential between CAR−/− and WT. In addition, the number of tartrate‐resistant acid‐phosphatase positive osteoclasts in the femur and serum level of CTx was not different between CAR−/− and WT mice. The higher BMD and microstructural parameters were not observed in female mice. Interestingly, serum level of testosterone in male CAR−/− mice was 2.5‐fold higher compared with WT mice and the mRNA expressions of Cyp2b9 and 2b10 in the liver, which regulate testosterone metabolism, were significantly down‐regulated in male CAR−/− mice. Furthermore, the difference in BMD between CAR−/− and WT mice disappeared at 8 weeks after performing orchiectomy. CAR−/− mice also exhibited significant increase in serum 1,25(OH)2D3 levels but Cyp 27B1 which converts 25(OH)D3 to 1,25(OH)2D3 was significantly down‐regulated compared to WT mice. These results suggest that in vivo deletion of CAR resulted in higher bone mass, which appears to be a result from reduced metabolism of testosterone due to down‐regulation of Cyp2b. J. Cell. Physiol. 229: 561–571, 2014. © 2013 Wiley Periodicals, Inc.
Mechanism‐based approach using a biomarker response to evaluate tocilizumab subcutaneous injection in patients with rheumatoid arthritis with an inadequate response to synthetic DMARDs (MATSURI study)
Authors: Ohta Shuji , Tsuru Tomomi , Terao Kimio , Mogi Seiji , Suzaki Midori , Shono Eisuke , Ishida Yoshimasa , Tarumi Eriko , Imai Masato 
Published: October 2013

AbstractA multicenter, open‐label, dose‐escalation phase 1/2 study was undertaken to evaluate the optimal subcutaneous tocilizumab dose that would result in exposure comparable to the intravenous tocilizumab 8‐mg/kg approved dose in patients with rheumatoid arthritis. A pharmacokinetic and biomarker approach was used to estimate the clinical optimal dose regimen of subcutaneous tocilizumab. Safety and efficacy of subcutaneous tocilizumab were assessed as secondary end points. Patients received subcutaneous tocilizumab at 81 mg every 2 weeks (q2w) (n = 8), 162 mg q2w (n = 12), or 162 mg weekly (qw) (n = 12) for 24 weeks. 88% of 162‐mg q2w patients and 100% of 162‐mg qw patients maintained mean serum trough tocilizumab concentrations of ≥1 µg/mL, and had exposure comparable with the approved intravenous tocilizumab dose of 8 mg/kg; this resulted in normalized C‐reactive protein levels and improvement in ACR20/50/70 responses. The most common adverse events were abnormal laboratory results, which were mild in severity. Anti‐tocilizumab antibodies were detected in a few patients in the 81‐mg q2w and 162‐mg qw groups. In conclusion, coupled with efficacy and tolerability results, the appropriate dose of subcutaneous tocilizumab was determined to be 162 mg q2w for Japanese patients.
Population pharmacokinetic and pharmacodynamic modeling of different formulations of ONO‐5334, cathepsin K inhibitor, in Caucasian and Japanese postmenopausal females
Authors: Hasegawa Chihiro , Ohno Tomoya , Umemura Takeo , Honda Naoki , Ohyama Michiyo , Nagase Shinichi , Small Maria , Deacon Steve , Ogawa Mikio , Ieiri Ichiro 
Published: October 2013

AbstractONO‐5334, a selective inhibitor of cathepsin K, is a potential new treatment for osteoporosis. The objectives of this study were to (1) develop population pharmacokinetic–pharmacodynamic (PK–PD) models for ONO‐5334 using dose‐ascending data from healthy postmenopausal females, (2) examine comparability of PK and/or PD profile between Caucasian and Japanese, and (3) compare PK–PD profile between immediate release tablet (IRT) and sustained release tablet (SRT). The population PK–PD models were developed for each formulation for post‐dose levels of bone resorption markers (serum CTX and NTX). The data were provided from 4 phase 1 studies with total of 201 Caucasian and 94 Japanese subjects. Plasma concentrations of ONO‐5334 and bone resorption markers were thoroughly evaluated in those studies. An indirect response model described relationships between bone resorption markers and plasma concentrations of ONO‐5334. There was no significant difference in PK and pharmacodynamic potency (IC50) between Caucasian and Japanese. Based on the developed model, serum CTX and NTX after administration of ONO‐5334 IRT or SRT were simulated, and the results showed that ONO‐5334 SRT would provide comparable PD effect on bone resorption markers with lower dose relative to IRT.
Impact of small‐for‐gestational age (SGA) status on gentamicin pharmacokinetics in neonates
Authors: Lulic‐Botica Mirjana , Sheer Terri , Edwards David , Thomas Ronald L. , Natarajan Girija 
Published: October 2013

AbstractWe compared gentamicin pharmacokinetics among neonates born small‐for‐gestational age (SGA) and appropriate for gestational age (AGA). We further compared gentamicin pharmacokinetics in subgroups of AGA and SGA neonates born preterm and term and treated within and after the initial week of age. Steady state peak and trough serum gentamicin concentrations were used to calculate clearance (Cl), elimination constant (Kel), volume of distribution (Vd), and half‐life (t1/2) in infants (n = 236) who received ≥48 hours therapy. Statistical analyses (SPSS 17.0) included chi‐square and the non‐parametric Mann–Whitney U‐test. SGA infants treated early (≤7days) (n = 29) and at postmenstrual ages ≤32 weeks (n = 23) had significantly lower median Kel (0.069/h vs. 0.081/h and 0.067/h vs. 0.075/h) and clearance (0.58 mL/kg/min vs. 0.68 mL/kg/min and 0.46 mL/kg/min vs. 0.65 mL/kg/min), compared to those born AGA. There were no significant differences in pharmacokinetic profiles with later therapy or at more mature ages. The prolonged half‐life of gentamicin may need to be considered in dosing regimens for preterm SGA infants in the initial week of life.
Simultaneous nutrient and carbon removal from azo dye wastewater using a photorotating biological contactor reactor
Authors: Wang Xingzu , Cheng Xiang , Sun Dezhi , Ren Yiwei , Xu Guihua 
Published: October 2013

AbstractBACKGROUNDBiological nutrient removal methods typically use separate anaerobic/anoxic and aerobic processes for the removal of nitrogen, phosphorus, or other nutrients from wastewaters. These processes require high operational and material costs. In this work, photosynthetic biofilm in a single photorotating biological contactor (PRBC) reactor was used to simultaneously remove carbon, nitrogen, phosphorus, and sulfur from high‐chroma‐dye wastewater.RESULTSSupplementary illumination of 12 h d−1 in PRBC readily enhanced photosynthetic bacterial activity, leading to 90.3% color removal, 93.9% chemical oxygen demand (COD) removal, and 94% 1‐2‐7‐triamino‐8‐hydroxy‐3‐6‐naphthalinedisulfate removal. Moreover, the removal efficiency of total nitrogen, phosphate, and sulfide at an illumination time of 12 h d−1 and a hydraulic retention time of 10 h was maintained at 69%, 72%, and 67%, respectively. Component analysis revealed that the protein, phosphorus, and sulfur in the photosynthetic biofilm were 67.5%, 2.4%, and 1.8%, respectively.CONCLUSIONCultivating photosynthetic biofilm in a single PRBC can achieve better efficiency of removal of carbon, nitrogen, phosphorus, and sulfur compared with biofilm in a rotating biological contactor. A feasible technology for removing nutrients and recycling useful elements from wastewater using photosynthetic biofilm in a single PRBC is proposed. © 2013 Society of Chemical Industry
Comparison of the methods for preparing a cordierite monolith‐supported Cu–Mn mixed‐oxide catalyst
Authors: Wu Dongfang , Li Wangbing , Gao Rui 
Published: October 2013

AbstractBACKGROUNDAn efficient, quick and simple preparation method is of great importance for mass production of monolithic catalysts in industry. This paper reports a comparison of three methods used to prepare a monolithic Cu–Mn mixed‐oxide/γ‐alumina/cordierite catalyst.RESULTSThe catalysts obtained by different methods have significant differences in catalytic activity and mechanical stability, although their chemical crystalline phases are identical. Similar to a conventional two‐step method, the simple one‐step sol dipping method leads to homogeneous distribution of active phase and hence to greater o‐xylene combustion activity, compared with the one‐step solution impregnation method. Furthermore, the one‐step sol dipping method results in the strongest adhesion and cohesion of catalytic material on to the monolithic substrate, followed by the one‐step solution impregnation method and then the conventional two‐step method.CONCLUSIONSThe one‐step sol dipping method is superior to the other two methods and is therefore recommended for use in the future preparation of monolithic catalysts, especially for mass production of monolithic catalysts in industry. © 2013 Society of Chemical Industry
Telesonography: Virtual 3D image processing of remotely acquired abdominal, vascular, and fetal sonograms
Authors: Arbeille Ph. , Fornage B. , Boucher A. , Ruiz J. , Georgescu M. , Blouin J. , Cristea J. , Carles G. , Farin F. , Vincent N. 
Published: October 2013

ABSTRACTPurposeTo design and test a new telesonography technique using remote volume acquisition by untrained operators in locations without access to trained sonographers, postprocessing, and interpretation done at expert centers.Materials and MethodsThe technique was tested with 84 sonograms of organs acquired in pregnant women (n = 8) and patients with various abdominal pathologic conditions (n = 11) located in French Guyana (France), Ceuta (Spain), and Murighiol (Romania). An operator inexperienced in sonography (US) placed the transducer over the predetermined acoustic window for each organ, then swept it from a −45° to a +45° position to scan the targeted organ. The acquired volume dataset was sent to an expert center via the Internet and reconstructed using a proprietary software, which allowed a trained sonographer to navigate through the appropriately reconstructed sonograms.ResultsAfter three‐dimensional processing at the expert center, the organs scanned in the obstetrical cases were adequately visualized by the expert in seven of eight (88%) examinations of the fetal head, femur, and umbilical cord and eight of eight (100%) examinations of the fetal abdomen and placenta, whereas in the general abdominal cases, the liver, gallbladder, portal vein, and right kidney were correctly visualized in 10 of 11 (91%) examinations.ConclusionsTelesonography allowed untrained operators to scan and transfer the US volume datasets over the Internet to an expert center where an expert sonographer could navigate through the reconstructed US volume and visualize sonograms of diagnostic quality. © 2013 Wiley Periodicals, Inc. J Clin Ultrasound 42:67–73, 2014
Synovial osteochondromatosis in the subacromial bursa mimicking calcific tendinitis: Sonographic diagnosis
Authors: Kim Tae‐Kwon , Lee Dae‐Hee , Park Jong‐Hoon , Kim Chul‐Hwan , Jeong Woong‐Kyo 
Published: October 2013

ABSTRACTSynovial osteochondromatosis is an idiopathic benign metaplasia of the synovial membrane rarely found in an extra‐articular bursa. We describe the case of a 55‐year‐old woman with synovial osteochondromatosis in the subacromial bursa mimicking calcific tendinitis. Plain radiographs showed a radiopaque mass over the middle facet of the greater tuberosity, suggesting calcific tendinitis. Sonography, however, showed a loose body in the subacromial bursa, and no evidence of calcification inside the rotator cuff. © 2013 Wiley Periodicals, Inc. J Clin Ultrasound 42:237–240, 2014
Virtual CT sonographically guided biopsy of a retroperitoneal mass in a patient with Erdheim‐Chester disease
Authors: Tombesi Paola , Di Vece Francesca , Rinaldi Rosa , Ermili Francesca , Sartori Sergio 
Published: October 2013

ABSTRACTImage fusion between sonography and CT allows real‐time synchronization of CT multiplanar reconstructed images with the corresponding sonographic images. This technique has mainly been used in liver imaging. We report the use of image fusion to target and successfully guide the percutaneous biopsy of a retroperitoneal a mass. This technique represents a promising tool in abdominal imaging, and it should be considered for the biopsy of lesions that are difficult to approach with conventional imaging guidance techniques. © 2013 Wiley Periodicals, Inc. J Clin Ultrasound 42:230–233, 2014
Do cortisol and corticosterone play the same role in coping with stressors? Measuring glucocorticoid serum in free‐ranging guanacos (Lama guanicoe)
Authors: Ovejero Ramiro , Novillo Agustina , Soto‐Gamboa Mauricio , Mosca‐Torres Maria E. , Cuello Pablo , Gregório Pablo , Jahn Graciela , Carmanchahi Pablo 
Published: October 2013

ABSTRACTHabitat can constrain and shape successful ecological and physiological strategies, thus providing the context for the evolution of life‐history traits. However, unpredictable challenges, such as storms, natural disasters, and human activities can also have great effects on stress. Glucocorticoids (GCs) are adrenal steroid hormones that play an important role in how vertebrates cope with these predictable and unpredictable environmental challenges. Although assessing GCs levels can have many applications in the study of wildlife and/or captive animals, with or without capturing individuals, it requires a species‐specific complete validation (analytical and biological) before its use. In this work, our aim was to: (a) validate a radioimmunoassay (RIA) for measuring GCs levels inL. guanicoeserum; (b) assess cortisol and corticosterone levels (if present) in serum of wildL. guanicoeindividuals; and (c) compare the response to acute stressors (handling, shearing, and release). Our results successfully: (a) validated RIA for asses GCs levels in wild ungulates; (b) confirmed the presence for cortisol and corticosterone and showed that both GCs are differently affected by environmental stimuli inL. guanicoe; and (c) showed that GCs exhibit different patterns in the field and in response to acute stressors, making these camelids an interesting endocrinological model when seeking the adaptive functions of a given variation and further emphasizing the complexity of GC physiology in wild mammals. J. Exp. Zool. 319A: 539–547, 2013. © 2013 Wiley Periodicals, Inc.