bluuebunny/crossref_metadata_2025_split huggingface dataset:
Our time will come: Is anuran community structure related to crop age?
Authors: Moreira Leonardo Felipe Bairos , Maltchik Leonardo 
Published: May 2015

AbstractAn ongoing controversy involves the debate about the effects of man‐made land transformation on freshwater biodiversity. It has been suggested that agricultural areas provide habitats for many species of amphibians, but crop age may affect richness and community structure. In such modified landscapes, a nested structure has been commonly detected, although community structure may be season specific and guild specific. Here, we determined detectability associated with site‐specific and survey‐specific variables and examined nestedness patterns in anuran communities in natural areas and rice fields with different crop ages (10 and 20 years) in southern Brazil. We studied whether nestedness was associated with time since cultivation and whether these patterns were similar across guilds in these areas. Anuran detectability was related only with time after sunset. Community composition varied between crop ages. Aquatic and arboreal species were associated with native areas and showed a nested pattern. Fossorial species did not show significant nestedness. Our results showed that factors associated with crop age may affect guilds in different ways. These effects seem to be related to individual traits of species (habitat preferences, reproductive modes, plasticity). Incorporating species traits may enhance conservation strategies in agroecosystems.
Effect of chronological addition of records to species distribution maps: The case of Tonatia saurophila maresi (Chiroptera, Phyllostomidae) in South America
Authors: Aguiar Ludmilla Moura de Souza , da Rosa Renato Oliveira Lopes , Jones Gareth , Machado Ricardo Bomfim 
Published: May 2015

AbstractEcological niche models have become very popular for analysing the potential distribution of species. Nevertheless, models are strongly influenced by many factors, such as spatial resolution, environmental variables and the quality of distribution records. In this paper, we evaluated how ecological niche models changed with the addition of records accumulated over four decades. Our model species was the stripe‐headed round‐eared bat (Tonatia saurophila). Thus, with data organized in chronological order, we could observe how the models changed in predicting distributions over time in comparison with all known point locations. We tested if partial models could predict the occurrence of new unpublished records for savannah areas in central Brazil, considering that the species is typically associated with forest environments. Our results indicate a high omission rate for models built with point localities from the 1970s and 1980s (58.5% and 50.0% of all known points respectively), and predicted that the species could occur in central Brazil. Although T. saurophila has indeed been recorded recently in central Brazil, it was found in places different from those predicted by the models using these restricted earlier data. Nevertheless, the environmental suitability of such areas is significantly different from sites largely described in earlier records from the Amazonia region, as shown by principal components analysis. We argue that populations of T. saurophila that occupy open habitats in central South America (including Caatinga, Cerrado, Chaco and semi‐deciduous interior forests) deserve further study at the genetic level to determine if bats in these very different habitats are taxonomically distinct from Amazonian populations. Our results also suggest that models based on very limited datasets for species occurrence can lead conservationists or decision makers to wrong conclusions.
Theorising about gender and computing interventions through an evaluation framework
Authors: Craig Annemieke 
Published: May 2015

AbstractDespite over 20 years of intervention programmes, the gender balance in the computing profession is not improving. It has been suggested that the problem with much of the research on gender and computing is that it is under‐theorised. The contribution of this study is an evaluation framework, designed to evaluate gender and computing interventions, which will advance the theorisation of research through programme evaluation. This study was undertaken in three phases: The first involved theory building through an extensive review of the literature resulting in a conceptual framework for intervention programme evaluation. The second phase consisted of a multiple‐case study of 14 major intervention programmes in Australia. Subsequent modifications to the conceptual framework resulted in the gender and computing intervention evaluation framework. The value of the framework was confirmed in phase three by intervention experts and showed that applying the framework will help programme champions to evaluate their programmes more thoroughly. The dissemination of sound evaluation results will then enable a deeper theorisation of the issues surrounding gender and computing interventions.
Influence of SLCO1B1 polymorphism on maintenance therapy for childhood leukemia
Authors: Suzuki Ryoko , Fukushima Hiroko , Noguchi Emiko , Tsuchida Masahiro , Kiyokawa Nobutaka , Koike Kazutoshi , Ma Enbo , Takahashi Hideto , Kobayashi Chie , Nakajima‐Yamaguchi Ryoko , Sakai Aiko , Saito Makoto , Iwabuchi Atsushi , Kato Keisuke , Nakao Tomohei , Yoshimi Ai , Sumazaki Ryo , Fukushima Takashi 
Published: May 2015

AbstractBackgroundManagement of the adverse effects of chemotherapy is essential to improve outcome of children with leukemia. Some genetic polymorphisms can predict treatment‐related toxicity, and be used individually in dose modification of 6‐mercaptopurine (6‐MP) and methotrexate (MTX) in maintenance therapy for childhood acute lymphoblastic leukemia (ALL). We investigated associations between clinical course and candidate gene polymorphisms less evaluated in Japanese patients.MethodsFifty‐three children who received maintenance chemotherapy were enrolled in this study. The scheduled dose of oral 6‐MP was 40 mg/m2 daily and that of oral MTX was 25 mg/m2 weekly. The doses were adjusted according to white blood cell count (target range, 2.5–3.5 × 109/L) and aspartate aminotransferase and alanine aminotransferase level (<750 IU/L). Eight polymorphisms in six candidate genes, TPMT, ITPA, MRP4, MTHFR, RFC1, and SLCO1B1, were genotyped using the Taqman PCR method. Clinical course was reviewed retrospectively from medical records.ResultsThe average dose of 6‐MP was lower in the patients with at least one variant allele at SLCO1B1 c.521 T > C than in the patients with wild homozygous genotype. The other analyzed polymorphisms were not associated with toxicity, 6‐MP, or MTX dose.ConclusionsPolymorphism of SLCO1B1 c.521 T > C could be a strong predictor of 6‐MP dose reduction in maintenance chemotherapy in childhood ALL.
Cervical Distraction‐Stabilization Using an Intervertebral Spacer Screw and String‐of Pearl (SOP™) Plates in 16 Dogs With Disc‐Associated Wobbler Syndrome
Authors: Solano Miguel A. , Fitzpatrick Noel , Bertran Judith 
Published: May 2015

ObjectiveTo retrospectively investigate a novel surgical technique using the Fitz Intervertebral Traction Screw (FITS) combined with string‐of‐pearl (SOP™) locking plates for the treatment of Disc Associated Wobbler Syndrome (DAWS).Study designCase series (n = 16).MethodsVentral approach and fenestration of the affected disc(s), nuclear extirpation, and insertion of the FITS device to the level of the dorsal annulus was followed by application of one or two 3.5 mm SOP™ plates. Autogenous cancellous bone graft was placed between adjacent vertebrae. Long‐term followup (>12months) via telephone or examination was attempted for all dogs.ResultsCase records of 16 dogs were reviewed. All dogs had Type II disc disease with a single site in 13 dogs and multiple sites in 3 dogs. Mean ± SD age was 75 ± 38 months and body weight was 34 ± 7 kg. Fifteen of the 16 dogs had significantly improved neurologic status (P =.01) at 6 weeks. Seven of the 16 dogs were examined at 24–36 months with 6 considered normal and 1 had mild ataxia. Postoperative radiographic intervertebral distraction was significant (P = .01). Radiographic bridging was recorded in 10/16 dogs at 6 weeks and in 7 dogs available for follow‐up at 5–36 months. Computed tomography in 3 dogs at 24–36 months and postmortem with histology in 1 dog confirmed bone‐bridging. Complications were not considered clinically significant and included implant loosening (2 dogs, both single plates) and minor end‐plate subsidence (8 dogs).ConclusionApplication of the FITS device in combination with 2 ventral SOP™ locking plates and autogenous cancellous bone graft was associated with excellent outcomes in dogs treated for DAWS in this small case series.
Multi-Purpose Fuel: Problems That We Face
Authors: Siemietkowski John S. 
Published: May 2015

A Pratt and Whitney FT4A Marine Gas Turbine Engine rated at 25,000 HP for a 100°F inlet day, was tested at the Naval Ship Engineering Center Philadelphia Division for a total of 201 hours, 15 minutes. The engine was subjected to an initial 30 hour “coking” run, conducted at 10,000 HP, 2380 rpm, to determine adverse effects on the engine under simulated destroyer type operation.
Following the 30 hour coking run, the engine was subjected to a 150 hour cycle endurance operation. Salt was admitted to the inlet air.
A combustion section inspection was performed at the end of the 30 hour coking run. No detrimental effects were noted at that time.
An overall combustion section inspection was performed at the end of the test. A fuel manifold and nozzle spray check was performed with both acceptable for further use. First stage turbine blades showed some degree of sulfidation, while the nozzle guide vanes showed evidence of coating loss and partial penetration into the base metal (on only the uncoated vanes).
The major problem area during the test was the failure of the coalescer fuel filter to function properly with Multi-Purpose fuel. Due to the higher pour point (with attendant “wax” precipitation) of the fuel in comparison with normal Marine Diesel (MIL-F-16884), plugging of the coalescer filter elements occurred in a very short period of time.
Engine performance over the entire test was satisfactory approximating that of previous FT4A testing.
Computation of Thermodynamic Properties of Carbon Dioxide in the Range 0–150 Deg C
Authors: Macchi E. , Angelino G. 
Published: May 2015

Methods for the computation of thermodynamic properties from volumetric measurements are presented and discussed. Their accuracy is evluated by means of an error analysis based on the generation of a set of pseudo-experimental points through an appropriate comptuer technique.
A set of very accurate density measurements is employed for the computation of thermodynamic functions of CO2 between 0 and 150 deg C including the two phase region.
The results of the calculations are reported on a series of tables.
The comparison of the computed functions with values reported by others suggests the conclusion that the tabulated data give reasonably accurate information about the thermal properties of CO2 as can be evaluated from the best density measurements available in the range 0–150 deg C.
Complete Genome Sequences for 59
Burkholderia
Isolates, Both Pathogenic and Near Neighbor
Authors: Johnson Shannon L. , Bishop-Lilly Kimberly A. , Ladner Jason T. , Daligault Hajnalka E. , Davenport Karen W. , Jaissle James , Frey Kenneth G. , Koroleva Galina I. , Bruce David C. , Coyne Susan R. , Broomall Stacey M. , Li Po-E , Teshima Hazuki , Gibbons Henry S. , Palacios Gustavo F. , Rosenzweig C. Nicole , Redden Cassie L. , Xu Yan , Minogue Timothy D. , Chain Patrick S. 
Published: May 2015

ABSTRACT

The genus
Burkholderia
encompasses both pathogenic (including
Burkholderia mallei
and
Burkholderia pseudomallei
, U.S. Centers for Disease Control and Prevention Category B listed), and nonpathogenic Gram-negative bacilli. Here we present full genome sequences for a panel of 59
Burkholderia
strains, selected to aid in detection assay development.
Draft Genome Sequence of Clostridium aceticum DSM 1496, a Potential Butanol Producer through Syngas Fermentation
Authors: Song Yoseb , Hwang Soonkyu , Cho Byung-Kwan 
Published: May 2015

ABSTRACT

Clostridium aceticum
DSM 1496 is a Gram-negative anaerobic chemolithoautotrophic acetogenic bacterium that is capable of producing commodity chemicals from syngas fermentation. In this study, we report the draft genome sequence of the
C. aceticum
DSM 1496 strain (4.16 Mb) to elucidate the syngas fermentation metabolic pathway.
Complete Genome Sequence of Cyanobacterium
Geminocystis
sp. Strain NIES-3709, Which Harbors a Phycoerythrin-Rich Phycobilisome
Authors: Hirose Yuu , Katayama Mitsunori , Ohtsubo Yoshiyuki , Misawa Naomi , Iioka Erica , Suda Wataru , Oshima Kenshiro , Hanaoka Mitsumasa , Tanaka Kan , Eki Toshihiko , Ikeuchi Masahiko , Kikuchi Yo , Ishida Makoto , Hattori Masahira 
Published: May 2015

ABSTRACT

The cyanobacterium
Geminocystis
sp. strain NIES-3709 accumulates a larger amount of phycoerythrin than the related NIES-3708 strain does. Here, we determined the complete genome sequence of the NIES-3709 strain. Our genome data suggest that the different copy number of rod linker genes for phycoerythrin leads to the different phycoerythrin contents between the two strains.
Abstract 1006: Structural peculiarities of flavonoids influence anti-angiogenic, cytotoxic and antioxidant effects: experimental and insilico analysis
Authors: Gacche Raju N. , Shegokar Harshala D. , Gond Dhananjay S. , Meshram Rohan J. 
Published: May 2015

Abstract
Structural diversity of flavonoids and biological activities has remained an important discourse in the mainstream of flavonoid research. In the present studies different class of flavonoids such as flavones, flavanones and flavanolols were evaluated for their antiangiogenic, cytotoxic and antioxidant activities. The anti-angiogenic activity was evaluated using in vivo chorioallantoic membrane model (CAM), antioxidant potential and kinetics of free radical scavenging activity was determined using DPPH (2, 2-diphenyl-1-picryl hydrazine) and superoxide anion radical (SOR) scavenging assays, while cytotoxicity against selected cancer cell lines was carried out using MTT cell viability assay. The physicochemical properties/quantum chemical discriptors of the selected flavonoids were calculated using BioMed CAChe 6.1.10 drug. The selected flavonoids were docked in silico onto the proangiogenic peptides such as vascular endothelial growth factor (VEGF), hypoxia inducible factor (HIF-1α), and vascular endothelial growth factor receptor-2 (VEGFR2) and others from human origin. The results of the present investigation are discussed in the mainstream of structure activity relationship which may be useful in translating flavonoids as therapeutic molecules targeting angiogenesis.
References
1. Gacche R. N. Shegokar Harshala, Gond Dhananjay , A. D. Jadhav and Ghole Vikram. (2010). Effect of Hydroxyl Substitution of Flavone on Antiangiogenic and Free Radical Scavenging Activities: A Structure Activity Relationship Studies Using Computational Tools. European J. Pharmaceutical Science 39, 37-44.
2. Gacche R. N. Shegokar Harshala, Gond Dhananjay , Zhenzhou Yang, A. D. Jadhav (2011). Evaluation of selected flavonoids as antiangiogenic, anticancer and radical scavenging agents: an experimental and in silico analysis. Cell Biochemistry and Biophysics 61, 651-663. DOI 10.1007/s12013-011-9252-z.
3. Gacche RN and Meshram RJ (2013) Targeting Tumor Micro-environment for Design and Development of Novel Anti-angiogenic Agents Arresting Tumor Growth. Progress in Biophysics & Molecular Biology 113 (2013) 333-354.
Note: This abstract was not presented at the meeting.
Citation Format: Raju N. Gacche, Harshala D. Shegokar, Dhananjay S. Gond, Rohan J. Meshram. Structural peculiarities of flavonoids influence anti-angiogenic, cytotoxic and antioxidant effects: experimental and insilico analysis. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1006. doi:10.1158/1538-7445.AM2014-1006
Abstract 1007: Nanoformulation enhances anti-angiogenic efficacy of Tunicamycin
Authors: Banerjee Aditi , Banerjee Dipak K. 
Published: May 2015

Abstract
The breast cancer landscape indicates it is a global problem and accounts for nearly a quarter of all cancers in women. In 2008, 1.4 million women were diagnosed with the disease worldwide and 438,000 died globally in 2010. In the United States, it is estimated that 296,000 women and 2,240 men will be diagnosed with breast cancer in 2013 and 39,620 women and 410 men will die from the disease. In 2030, with no major changes in prevention or treatment 747,802 women are estimated to die worldwide. Breast cancer treatment includes surgery, radiation therapy, chemotherapy and/or hormonal therapy, and within the past 15 years, targeted antibody therapy. Unfortunately, de novo and acquired resistance are major issues with all known targeted therapies. Tumor microenvironment (TME) is immensely complex and contributes to chemoresistance. Using Tunicamycin, a potent inhibitor of asparagine-linked (N-linked) protein glycosylation we have established earlier that Tunicamycin inhibits (i) angiogenesis in vitro by arresting cells in G1; (ii) in vivo angiogenesis in Matrigel™ implant in nude mice; and (iii) prevents the progression of double- and triple-negative breast tumors in athymic nude mice by inducing “ER stress”. Nanoparticles (&lt;100 nm) evade the immune system's clearing mechanisms long enough to reach the targeted disease tissue efficiently. We have, therefore, hypothesized that nano-formulated Tunicamycin would have a better efficacy in treating breast cancer in the clinic. The objective was to evaluate nano-formulated Tunicamycin, and we have used Tunicamycin encapsulated in peptide nanotubes, nanotubes bound to gold nanoparticles (Au NPs) conjugated with Tunicamycin, Tunicamycin conjugated with nanotubes, Au NPs bound to tubes and conjugated with Tunicamycin, and Au NPs conjugated with Tunicamycin. The MTT assay indicated that nanoparticles (1 μg/mL) inhibited capillary endothelial cells proliferation ∼50% within one hour of treatment whereas the native Tunicamycin had no effect. The nanoformulated Tunicamycin blocked the cell cycle progression by inhibiting either both cyclin D1 and CDK4, or cyclin D1, or the CDK4 expression as well as the expression of phospho Rb (serine-229/threonine-252). Phosphorylation of p53 at serine-392 was down-regulated but not the total p53. Increased expression of GRP-78/Bip identified “ER stress”. Upregulated expression (1.6-5.5 folds) of phopsho-PERK supported induction of unfolded protein response (upr) signaling. Down regulated expression of caspase-9 and caspase-3 proposes a non-canonical pathway of cell death during “ER stress” induced by nano-formulated Tunicamycin.
Citation Format: Aditi Banerjee, Dipak K. Banerjee. Nanoformulation enhances anti-angiogenic efficacy of Tunicamycin. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1007. doi:10.1158/1538-7445.AM2014-1007
Abstract 1013: Angiopoietin 1/2 inhibition impairs tumor growth in an orthotopic model of renal cell carcinoma
Authors: Orillion Ashley , Miles Kiersten Marie , Shen Li , Adelaiye Remi , Ciamporcero Eric , Ramakrishnan Swathi , Ku Sheng Yu , Elbanna May , Chintala Sreenivasulu , Pili Roberto 
Published: May 2015

Abstract
Background: Clear cell renal cell carcinoma (ccRCC), the most common form of renal cell carcinoma, is characterized VEGF driven tumor vascularization. However, in patients treated with anti-VEGF therapies inevitably tumor progression occurs, likely driven by up-regulation of alternative proangiogenic pathways including angiopoietins and c-MET. We hypothesized that angiopoietin inhibition has anti-tumor effect on RCC and may affect the development of resistance to anti-VEGF therapies. In the current study we utilized the dual angiopoietin inhibitor AMG 386 in murine models of RCC, and assessed the preclinical impact of this strategy. Methods: Seven week old female Balb/c mice were injected with 250,000 RENCA cells (luciferase expressing), one week later these mice were randomized, using bioluminescence imaging into two treatment groups: vehicle or Amgen 386 (5.6mg/kg/day, twice a week, subQ injection). Body weight measurements, tumor growth rate, and end point tumor weight measurements were used to evaluate possible side effects and the progression of RENCA tumors. Four weeks of treatment culminated in collection of tumor tissues for immunohistochemistry analysis of Ki67 and CD31. In addition we have ongoing studies utilizing patient derived xenografts (PDX) of ccRCC developed in our lab. Results: In the RENCA model AMG 386 induced a significant decrease of tumor weight by 44.0 percent (p = 0.0123). In addition, histological analysis of tumor sections revealed a high proportion of necrosis and decreased blood vessel density. Studies are ongoing with sunitinib sensitive PDXs and matched sunitinib resistant PDXs treated with AMG 386 in combination with a selective c-MET inhibitor. Results from these models will be available at the time of the meeting. Conclusions: Our preliminary results suggest that AMG 386 as a single agent is inhibiting both vascularization and tumor growth in an orthotopic renal cell carcinoma model. Ongoing studies with AMG 386 in combination with a selective c-MET inhibitor in sunitinib sensitive and resistant PDX models will provide guidance for the optimal clinical development of angiopoietin inhibitors in patients with ccRCC.
Citation Format: Ashley Orillion, Kiersten Marie Miles, Li Shen, Remi Adelaiye, Eric Ciamporcero, Swathi Ramakrishnan, Sheng Yu Ku, May Elbanna, Sreenivasulu Chintala, Roberto Pili. Angiopoietin 1/2 inhibition impairs tumor growth in an orthotopic model of renal cell carcinoma. [abstract]. In: Proceedings of the 105th Annual Meeting of the American Association for Cancer Research; 2014 Apr 5-9; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2014;74(19 Suppl):Abstract nr 1013. doi:10.1158/1538-7445.AM2014-1013