bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inactivation of Myosin Binding Protein C Homolog in Zebrafish as a Model for Human Cardiac Hypertrophy and Diastolic Dysfunction
Authors: Chen Yau‐Hung , Pai Chiung‐Wen , Huang Shu‐Wei , Chang Sheng‐Nan , Lin Lian‐Yu , Chiang Fu‐Tien , Lin Jiunn‐Lee , Hwang Juey‐Jen , Tsai Chia‐Ti 
Published: September 2013

Background
Sudden cardiac death due to malignant ventricular arrhythmia is a devastating manifestation of cardiac hypertrophy. Sarcomere protein myosin binding protein C is functionally related to cardiac diastolic function and hypertrophy. Zebrafish is a better model to study human electrophysiology and arrhythmia than rodents because of the electrophysiological characteristics similar to those of humans.

Methods and Results

We established a zebrafish model of cardiac hypertrophy and diastolic dysfunction by genetic knockdown of myosin binding protein C gene (
mybpc3
) and investigated the electrophysiological phenotypes in this model. We found expression of zebrafish
mybpc3
restrictively in the heart and slow muscle, and
mybpc3
gene was evolutionally conservative with sequence homology between zebrafish and human
mybpc3
genes. Zebrafish with genetic knockdown of
mybpc3
by morpholino showed ventricular hypertrophy with increased myocardial wall thickness and diastolic heart failure, manifesting as decreased ventricular diastolic relaxation velocity, pericardial effusion, and dilatation of the atrium. In terms of electrophysiological phenotypes,
mybpc3
knockdown fish had a longer ventricular action potential duration and slower ventricular diastolic calcium reuptake, both of which are typical electrophysiological features in human cardiac hypertrophy and heart failure. Impaired calcium reuptake resulted in increased susceptibility to calcium transient alternans and action potential duration alternans, which have been proved to be central to the genesis of malignant ventricular fibrillation and a sensitive marker of sudden cardiac death.

Conclusions

mybpc3
knockdown in zebrafish recapitulated the morphological, mechanical, and electrophysiological phenotypes of human cardiac hypertrophy and diastolic heart failure. Our study also first demonstrated arrhythmogenic cardiac alternans in cardiac hypertrophy.
The Martyrdom of Polycarp in Church Slavonic: An Evidence of the Academic Menologion
Authors: Khomych Taras 
Published: September 2013

Abstract
This contribution deals with a significant gap in the studia Polycarpiana. It focuses, in particular, on a Church Slavonic translation of the Martyrdom of Polycarp, one of the earliest of Christian martyria. This versional evidence has not been published until now and, consequently, not taken into account by any of the previous editions of the Martyrdom. The present article offers a collation and a detailed analysis of the earliest available copy of the translation. Eventually it shows that the Slavonic version may provide an independent (from the extant Greek manuscripts) testimony to the text of the Martyrdom and as such is of significant text-critical value.
Condemning Nature? Natura and Asceticism in the Jovinian Affair
Authors: Hunt Thomas E. 
Published: September 2013

Abstract
Writing and preaching at Rome in the 390s, Jovinian argued that ascetic Christianity was based on a heretical denial of the good of God’s creation. This article points out that conformity to nature (natura) was a key element of Jovinian’s teaching as it comes down to us through Jerome. Jovinian taught that marriage was a part of human nature and demonstrably good as part of God’s creation. The word natura was also important in Jerome’s arguments against Jovinian. To refute Jovinian, Jerome offered a vision of human nature based not in empirical observation, nor in social custom, but in the actions of the incarnate Christ. In so doing, he challenged notions of human nature and social custom circulating in contemporary Rome.
Representing Equilibrium and Nonequilibrium Convection in Large-Scale Models
Authors: Bechtold Peter , Semane Noureddine , Lopez Philippe , Chaboureau Jean-Pierre , Beljaars Anton , Bormann Niels 
Published: September 2013

AbstractA new diagnostic convective closure, which is dependent on convective available potential energy (CAPE), is derived under the quasi-equilibrium assumption for the free troposphere subject to boundary layer forcing. The closure involves a convective adjustment time scale for the free troposphere and a coupling coefficient between the free troposphere and the boundary layer based on different time scales over land and ocean. Earlier studies with the ECMWF Integrated Forecasting System (IFS) have already demonstrated the model’s ability to realistically represent tropical convectively coupled waves and synoptic variability with use of the “standard” CAPE closure, given realistic entrainment rates.A comparison of low-resolution seasonal integrations and high-resolution short-range forecasts against complementary satellite and radar data shows that with the extended CAPE closure it is also possible, independent of model resolution and time step, to realistically represent nonequilibrium convection such as the diurnal cycle of convection and the convection tied to advective boundary layers, although representing the late night convection over land remains a challenge. A more in-depth regional analysis of the diurnal cycle and the closure is provided for the continental United States and particularly Africa, including comparison with data from satellites and a cloud-resolving model (CRM). Consequences for global numerical weather prediction (NWP) are not only a better phase representation of convection, but also better forecasts of its spatial distribution and local intensity.
Assessing the Influence of Regional SST Modes on the Winter Temperature in China: The Effect of Tropical Pacific and Atlantic
Authors: Jiang Zhihong , Yang Hao , Liu Zhengyu , Wu Yanzhu , Wen Na 
Published: September 2013

Abstract
This study investigates the influence of different sea surface temperature (SST) modes on the winter temperature in China using the generalized equilibrium feedback assessment (GEFA). It is found that the second EOF mode of winter temperature in China during 1958–2010 shows a typical northeast–southwest (NE–SW) pattern, which is a major spatial mode of Chinese winter temperature at interannual scales. The winter temperature of the NE–SW pattern is forced mainly by SST modes in the tropical Pacific and Atlantic. For 2009/10, the tropical Pacific El Niño mode and tropical Atlantic tripole mode have the largest contribution to the response. The physical mechanism of the cold northeast–warm southwest (CNE–WSW) pattern is also explained in terms of GEFA of the responses of the atmospheric circulation. The northerly flow at the low level transports cold air to northern and northeastern China, resulting in a lower temperature there. Meanwhile, the anomaly meridional wind advects warm air from the southern oceans to southwestern China, leading to warming there.
Peri-operative troponin monitoring using a prototype high-sensitivity cardiac troponin I (hs-cTnI) assay: comparisons with hs-cTnT and contemporary cTnI assays
Authors: Lee Graham R , Jhanji Shaman , Tarrant Heloise , James Steve , Pearse Rupert M , Fitzgibbon Maria 
Published: September 2013

Background Non-cardiac surgery is associated with major vascular complications and higher incidences of elevated plasma troponin (cTn) concentration. Goal-directed therapy (GDT) is a stroke volume (SV)-guided approach to intravenous (IV) fluid therapy that improves tissue perfusion, oxygenation and reduces post-operative complications. In patients undergoing major gastro-intestinal surgery, we compared high sensitive and contemporary troponin assays and correlated results with patient outcome. Methods Patients ( n = 135) were randomized to receive IV fluid, guided by either the central venous pressure (CVP group, n = 45) or SV (± dopexamine inotrope, n = 45 per group). Serum was obtained pre- and post-operatively (0, 8 and 24 h) for troponin analysis by a prototype hs-cTnI assay (Abbott Laboratories), hs-cTnT (Roche Diagnostics) and contemporary cTnI (Beckman Coulter) assays. Results All troponin measurements were increased ( P ≤ 0.05) post-operatively but there was no difference ( P > 0.05) amongst treatments. Post-operative increases were reported more frequently ( P ≤ 0.05) and earlier with hs-cTnI. Temporal increases ( P ≤ 0.05) were reported in patients with and without complications for hs-cTnI/T assays but only in the complications group for cTnI measurements. Elevations ≥99th centile occurred most often ( P ≤ 0.05) for hs-cTnT measurements but with similar frequency for both outcome groups (all assays). Only the hs-cTnI assay showed an increased relative risk of mortality ( P ≤ 0.05) for elevations ≥99th centile Conclusions Our study may suggest a possible preference for the hs-cTnI assay in the peri-operative setting; however, our findings should be verified for larger cohort studies where emerging reference range data is incorporated for improving risk prediction with hs-cTn assays.
Detection of cytochrome P450 2C19 gene polymorphism from noninvasive samples by cycling probe technology
Authors: Nakagawa Saori , Kuwabara Naoko , Takamatsu Yu , Shimoeda Sadahiko , Ohta Shin , Yamato Susumu 
Published: September 2013

Background The proportion of poor metabolizers (PMs) of cytochrome P450 (CYP) 2C19 is much higher in the Japanese population than in European populations. Cycling probe technology (CPT) is a simple signal amplification technique for targeting specific DNA sequences. CPT utilizes a chimeric DNA-RNA-DNA probe that is cleaved by the enzyme ribonuclease (RNase H). In this study, using CPT, we aimed to detect the CYP2C19 gene polymorphism from noninvasive samples to determine extensive metabolizers (EMs) and PMs of CYP2C19. Methods DNA samples were extracted from hair, buccal mucosa, and blood cells. Primers and cycling probes were designed specifically for region G636A for exon 4 and G681A for exon 5, reported to be gene polymorphisms of CYP2C19. Results DNA extracted from hair follicle cells and buccal epithelial cells was the same as that collected from invasive blood sampling. The genotype of CYP2C19 was successfully identified as either EM or PM in 71 samples, producing identical results to those for the TaqMan method, except in three samples. Conclusions We successfully detected the two gene polymorphisms of CYP2C19 from noninvasive samples using a simple DNA extraction method and CPT.
Postdischarge Complications Predict Reoperation and Mortality after Otolaryngologic Surgery
Authors: Chen Michelle M. , Roman Sanziana A. , Sosa Julie A. , Judson Benjamin L. 
Published: September 2013

Objectives(1) Determine procedure‐specific rates of postdischarge complications (PDCs) and their risk factors in the first 30 days following inpatient otolaryngologic surgery. (2) Evaluate association between PDCs and risk of reoperation and mortality.Study DesignRetrospective cohort study.SettingAmerican College of Surgeons National Surgical Quality Improvement Program (2005‐2011).Subjects and MethodsWe identified 48,028 adult patients who underwent inpatient otolaryngologic surgery. Outcomes of interest included complications, reoperation, and mortality in the first 30 days following surgery. Statistical analysis included chi‐square, t tests, and multivariate regression.ResultsLaryngectomy, lip, and tongue/floor of mouth surgery had the highest PDC rates (8.0%, 7.4%, and 4.1%, respectively). Within the first 48 hours, week, and 2 weeks post discharge, 10%, 44%, and 73% of PDCs occurred, respectively. Common PDCs included surgical site infections (53.6%), other infections (37.4%), and venous thromboembolic events (7.4%). Multivariate analysis demonstrated that increasing age (odds ratio [OR] = 1.01; 95% confidence interval [CI], 1.01‐1.02), prolonged operative time (OR = 1.68; 95% CI, 1.39‐2.03), hospital stay >1 day (OR = 1.49; 95% CI, 1.18‐1.86), and American Society of Anesthesiologists (ASA) class ≥3 (OR = 1.45; 95% CI, 1.18‐1.78) were independently associated with PDCs. Patients with PDCs were more likely to die (0.9% vs 0.1%, P <. 001) or have a reoperation (10.4% vs 1.2%, P <. 001).ConclusionThis is the first study of overall postdischarge events after otolaryngologic surgery. PDC rates in otolaryngology occur soon after discharge, are procedure specific, and are associated with reoperation and mortality. Targeted procedure‐specific triage and follow‐up plans for high‐risk patients may improve outcomes.
Impact of Store Personality Antecedents on Store Personality Dimensions: An Empirical Study of Department Retail Brands
Authors: Das Gopal , Guin Kalyan Kumar , Datta Biplab 
Published: September 2013

The objective of this study was to explore the impact of store personality antecedents on store personality dimensions. A mall-intercept survey was undertaken using a systematic sampling of department store shoppers of age 18 years and above in Kolkata, a metropolitan city of India. Questionnaire was used to collect data from busy shopping malls or centres located in different places of Kolkata with systematic sampling. The impact of store personality antecedents on store personality dimensions was explored using stepwise regression analysis. Results revealed that different sets of store personality antecedent affect various department store personality dimensions differently. The impacts of all sets are positive and significant. Arguably, this article was the first to explore the link between store personality antecedents and store personality dimensions in Indian and department store personality contexts.
Stent Graft in the Treatment of Pseudoaneurysms of the Hepatic Arteries
Authors: Lü Peng-Hua , Zhang Xi-Cheng , Wang Li-Fu , Chen Zhao-Lei , Shi Hai-Bin 
Published: September 2013

Objective: The purpose of our study was to evaluate the technical feasibility, procedural complications, clinical follow-up, and computed tomography (CT) scan outcomes of hepatic artery pseudoaneurysms (HAPs) treated with stent graft. Methods: Between October 2004 and October 2009, we treated 8 patients with HAPs with stent graft. Results: Stent graft deployment was technically successful in all the patients. Complete exclusion of the pseudoaneurysm preserving patency of the hepatic artery was achieved in 6 patients. Total occlusion of the common hepatic artery was observed in 1 patient, and vasospasm of proper hepatic artery and endoleak from distal stent margin were observed in another patient. The 2 patient were controlled through occlusion parent artery with coils. After these procedures, symptoms of bleeding and abdominal pain disappeared. Follow-up enhanced CT scan was performed at an average of 14 months (range, 6-26months), which showed complete disappearance of the HAP and patency of the stent without intrastent stenosis in 6 patients who had successful deployment of the stent. Conclusions: Endovascular treatment of HAPs using stent graft can maintain the hepatic artery blood flow and could be considered as an alternative to embolization.
Sequential Compression Biomechanical Device Versus Primary Amputation in Patients With Critical Limb Ischemia
Authors: Tawfick Wael A. , Hamada Nader , Soylu Esraa , Fahy Anne , Hynes Niamh , Sultan Sherif 
Published: September 2013

Introduction: Patients with critical limb ischemia (CLI), who are unsuitable for intervention, face the consequence of primary amputation. Sequential compression biomechanical device (SCBD) therapy provides a limb salvage option for these patients. Objectives: To assess the outcome of SCBD in patients with severe CLI who are unsuitable for revascularization. Primary end points were limb salvage and 30-day mortality. Methods: From 2005 to 2012, 189 patients with severe CLI were not suitable for revascularization. In all, 171 joined the SCBD program. We match controlled 75 primary amputations. Results: All patients were Rutherford category 4 or higher. Sustained clinical improvement was 68% at 1 year. Mean toe pressure increased from 19.9 to 35.42 mm Hg, P < .0001. Mean popliteal flow increased from 35.44 to 55.91 cm/sec, P < .0001. The 30-day mortality was 0.6%. Limb salvage was 94% at 5 years. Freedom from major adverse clinical events was 62.5%. All-cause survival was 69%. Median cost of managing a primary amputation patient is €29 815 compared to €3985 for SCBD. We treated 171 patients with artassist at a cost of €681 965. However, primary amputation for 75 patients cost €2 236 125. Conclusion: The SCBD therapy is a cost-effective and clinically effective solution in patients with CLI having no option of revascularization. It provides adequate limb salvage while providing relief of rest pain without any intervention.
Spinal cord stimulation: Subjective pain intensity and presurgical correlates in chronic pain patients
Authors: Sumner Lekeisha A , Lofland Kenneth 
Published: September 2013

Objectives This study determined the changes in pain intensity prior to and after permanent placement of spinal cord stimulation (SCS), as measured by the Visual Analog Scale (VAS) and, examined associations of presurgical characteristics to both pre- and post-SCS VAS scores. Materials and methods Demographic, disease, and psychological data were extracted from the medical charts of chronic pain patients ( N = 58) being treated at a Hospital in IL, USA. Most patients were diagnosed with complex regional pain syndrome (51.9%) and low back pain (33.3%). The majority were White (72.5%), male (60%), married (72%), and temporary disabled or receiving worker’s compensation (40%). Study objectives were tested with correlational and univariate analysis. Results A significant reduction between pre ( M = 6.87; SD = 1.38) and Post-( M = 4.11; SD = 1.54) SCS pain intensity scores was observed ( p < .000). Pre-SCS pain intensity scores were associated with medical diagnosis ( p < .01), marital status ( p < .05), and catastrophizing ( p < .05). Post-SCS pain intensity scores were associated with body mass index ( p < .05), medical diagnosis ( p < .05), employment status ( p < .05), bodily concern ( p < .05), marital status ( p < .05), and race ( p < .05). Conclusions Results strengthen support for the short-term effectiveness of SCS and demonstrate the differential associations of presurgical factors on pain intensity.
Increased International Normalized Ratio in a Patient Taking Warfarin and Celecoxib
Authors: O'Donnell Dannielle C , Hooper Justin S 
Published: September 2013

Objective: To report a case of increased international normalized ratio (INR) associated with the addition of celecoxib therapy. Case Summary: A 77-year-old white woman who was previously stabilized at a target INR of 2–3 on warfarin 26.25 mg/wk for recurrent atrial fibrillation presented with “leg pain.” Celecoxib 100 mg twice daily orally was added to her regimen. After approximately three weeks of celecoxib therapy, her INR was elevated at 3.5, with no signs of bleeding. Her warfarin was withheld for one day and reinitiated at 23.75 mg/wk (∼10% decrease). Follow-up INRs at one and three weeks had returned to target INR range between 2 and 3. Discussion: Drug interactions are common with warfarin due to induction or inhibitions of the cytochrome P450 system and changes in protein binding. Although the celecoxib package insert states that there is not a significant interaction in healthy subjects receiving 2–5 mg/d of warfarin, the potential exists, as both are highly protein bound and metabolized through CYP2C9. Conclusions: This case report suggests that the INR may be increased after celecoxib initiation in patients undergoing anticoagulation. This may heighten the risk for clinically significant bleeding in patients previously stabilized on a warfarin regimen. Clinicians should consider more frequent INR monitoring of patients receiving warfarin after celecoxib is initiated.
Absorption of Clonidine from a Halved Transdermal System — A Pilot Study
Authors: Wong Nina N , Chen Julie L , Luks-Golger Denise 
Published: September 2013

Objective: To assess whether clonidine bioavailability is compromised when a transdermal system is cut in half. Methods: Seven healthy volunteers were enrolled in this prospective crossover study. In phase I, an intact clonidine 0.1 mg/24 h transdermal system (TS-1) was applied and plasma clonidine concentration was obtained 72 hours after application, after steady-state (48–72 hours) was purported to have been reached. After a minimum seven-day washout period, half of a clonidine 0.2 mg/24 h transdermal system (½TS-2) was applied in phase II, with plasma clonidine concentrations obtained at 24, 48, 72, 120, and 192 hours following application. Results: Mean plasma clonidine concentrations at 72 hours with TS-1 in phase I were 0.17 ± 0.07 ng/mL. Mean plasma clonidine concentrations at 24, 48, 72, 120, and 192 hours with ½TS-2 in phase II were 0.16 ± 0.11, 0.15 ± 0.06, 0.15 ± 0.05, 0.19 ± 0.4, and 0.20 ± 0.8 ng/mL, respectively. There was no statistically significant difference between mean concentrations at 72 hours in phases I and II, but individual clonidine concentrations between phases varied 50–286%. Individual concentration versus time curves obtained from subjects in phase II lacked a consistent pattern. Conclusions: Although there was no statistically significant difference in mean concentrations at 72 hours, individual clonidine concentration variations may have a clinically significant impact. The lack of a consistent plasma concentration versus time pattern may also be of clinical concern. Based on these results, cutting the clonidine transdermal system may compromise its integrity, and is therefore not recommended.
Discontinuity of Outpatient Antipsychotic Pharmacotherapy: Risperidone Maintenance after Hospitalization
Authors: Malan Randy D , Luchins Daniel J , Fichtner Christopher G , Hanrahan Patricia , Klass David B 
Published: September 2013

Objective: To investigate continuity of outpatient antipsychotic pharmacotherapy with risperidone in patients discharged from state psychiatric hospitals. Methods: Of 1,201 patients discharged from Illinois state psychiatric hospitals while taking risperidone during the first year of the medication's commercial availability, 627 public aid–eligible patients were trackable on the basis of public aid billing data. Data on risperidone continuation and other medication use for these patients were collected for the two-year period following each patient's discharge. Results: Forty-four percent of the patients discontinued risperidone within two weeks of hospital discharge, with one-half receiving no medications and most of the remainder switching to other antipsychotics. More than 75% of the patients discontinued risperidone within one year of hospital discharge, and fewer than 1% received continuous risperidone pharmacotherapy for the two-year follow-up period. Conclusions: Although the study methods do not permit us to draw conclusions regarding specific reasons for risperidone discontinuation in this patient sample, 66% of whom had a diagnosis of schizophrenia or schizoaffective disorder and 86% of whom had psychotic diagnoses, the rapid drop-off and discontinuity in medication management following hospital discharge is almost certainly inconsistent with optimum clinical care for this group of patients. Discontinuities in maintenance pharmacotherapy may compromise medical effectiveness, even for newer antipsychotic medications such as risperidone.