bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of loneliness and social isolation after spousal loss: A systematic review of longitudinal studies on widowhood
Authors: Niino Kerri , Patapoff Molly A. , Mausbach Brent T. , Liu Hui , Moore Alison A. , Han Benjamin H. , Palmer Barton W. , Jester Dylan J. 
Published: August 2024

AbstractBackgroundSpousal loss is a stressful life event that is associated with loneliness and social isolation, both of which affect mental and physical health. The primary objective of this paper was to synthesize longitudinal studies that investigated loneliness and social isolation in widowhood.MethodsA systematic search of the literature was conducted using three electronic databases. 26 longitudinal studies published through June 2024 were included for further analysis. Participant characteristics, study design, and key findings were extracted.ResultsMost studies were from the United States or Europe, included more widows than widowers, and assessed loneliness in older adults aged >60 years. Loneliness peaked directly following spousal death, but findings were inconsistent regarding the lasting effects of widowhood. Heterogeneity in the longitudinal trajectories of loneliness was noted, with studies showing linear increases, decreases, or curvilinear relationships over time. Several factors modified the relationship between widowhood and loneliness, including volunteerism, military experience, income, and age. Widowers consistently reported greater loneliness and worse social isolation when compared with widows. Few studies investigated social isolation specifically, but those that did found that social isolation may decrease in widowhood.ConclusionsAs the world grapples with a social pandemic of loneliness and social isolation, widowed adults may be uniquely affected. Few studies investigated the longitudinal trajectory of loneliness and especially social isolation in widowhood, and those that did found heterogenous results. Future work is needed to understand why some widowed adults are uniquely affected by feelings of loneliness and social isolation while others are not, and whether potentially modifiable factors that moderate or mediate this relationship could be leveraged by psychosocial interventions.
Corruption dynamics and political instability
Authors: Lisciandra Maurizio , Miralles Asensio Antonio , Monteforte Fabio 
Published: August 2024

AbstractThis paper introduces a model where a briber designs a bribing schedule targeted at the governing party within a bipartisan system to secure favorable treatment. Detected corruption increases voters' resentment, and in turn, the risk of political turnover—raising the minimum acceptable bribe. Periods without corruption mitigate such a risk. Should the briber deem bribing unprofitable for sufficiently high levels of resentment, resentment converges to a steady state in finite time. Conversely, if the briber perceives bribing as profitable regardless of the resentment level, the dynamics may result in continuous bribing and an unbounded increase in resentment (exploding dynamics). The model underscores the complexity of addressing corruption, emphasizing the need to balance reducing corruption with preventing excessive political instability. Societal forgiveness and sensitivity significantly shape corruption dynamics and public resentment. While forgiveness reduces long‐run resentment, it concurrently exacerbates long‐term corruption and, on balance, may have a detrimental effect on long‐term welfare. Sensitivity has no long‐run effect on resentment, while it reduces both corruption activity and political instability in the long term. Finally, exogenous political instability exacerbates corruption, resentment, and the risk of exploding dynamics.
Geographic patterns and climatic drivers of phylogenetic structure of liverworts along a long elevational gradient in the central Himalaya
Authors: Qian Hong 
Published: August 2024

AbstractFor clades originating in warm climates, the tropical niche conservatism hypothesis predicts that current biological assemblages in colder or drier climates are expected to have lower phylogenetic diversity, and species in colder or drier climates are expected to be more closely related to each other (i.e., higher phylogenetic clustering). Liverworts are one of the oldest clades of extant land plants. They originated about 500 Ma during a warm (“greenhouse”) period and experienced multiple major cycles of warm and cold periods. Here, I test the tropical niche conservatism hypothesis using liverwort assemblages distributed along an elevational gradient crossing about 5000 m of elevation in the central Himalaya. I found that, in general, phylogenetic diversity and dispersion decrease with increasing elevation and thus with decreasing temperature, which is consistent with the tropical niche conservatism hypothesis. Phylogenetic diversity decreases with elevation monotonically, but phylogenetic dispersion decreases with elevation in a triphasic (zig‐zag) pattern, which is generally consistent with the triphasic pattern found in angiosperms and polypod ferns along the same elevational gradient. Temperature‐related variables explained approximately the same amount of the variation in phylogenetic diversity and dispersion as did precipitation‐related variables, although mean annual temperature explained 9%−15% more variation than did annual precipitation. Climate extreme variables explained approximately the same amount of variation in phylogenetic diversity and dispersion as did climate seasonality variables.
Post‐transfer adaptation of HGT‐acquired genes and contribution to guanine metabolic diversification in land plants
Authors: Wu Jun‐Jie , Deng Qian‐Wen , Qiu Yi‐Yang , Liu Chao , Lin Chen‐Feng , Ru Ya‐Lu , Sun Yue , Lai Jun , Liu Lu‐Xian , Shen Xing‐Xing , Pan Ronghui , Zhao Yun‐Peng 
Published: August 2024

Summary

Horizontal gene transfer (HGT) is a major driving force in the evolution of prokaryotic and eukaryotic genomes. Despite recent advances in distribution and ecological importance, the extensive pattern, especially in seed plants, and post‐transfer adaptation of HGT‐acquired genes in land plants remain elusive.

We systematically identified 1150 foreign genes in 522 land plant genomes that were likely acquired via at least 322 distinct transfers from nonplant donors and confirmed that recent HGT events were unevenly distributed between seedless and seed plants.

HGT‐acquired genes evolved to be more similar to native genes in terms of average intron length due to intron gains, and HGT‐acquired genes containing introns exhibited higher expression levels than those lacking introns, suggesting that intron gains may be involved in the post‐transfer adaptation of HGT in land plants.

Functional validation of bacteria‐derived gene GuaD in mosses and gymnosperms revealed that the invasion of foreign genes introduced a novel bypass of guanine degradation and resulted in the loss of native pathway genes in some gymnosperms, eventually shaping three major types of guanine metabolism in land plants. We conclude that HGT has played a critical role in land plant evolution.
Clinical outcomes for minimally invasive sacroiliac joint fusion with allograft using a posterior approach
Authors: Moghim Robert , Bovinet Chris , Jin Max Y. , Edwards Katie , Abd‐Elsayed Alaa 
Published: August 2024

AbstractBackgroundSacroiliac joint (SIJ) dysfunction can occur as a result of injury, degeneration, or inflammation. This dysfunction presents symptoms of pain at various locations, including the low back, hips, buttocks, and legs. The diagnosis of SIJ dysfunction is challenging and cannot be achieved solely with imaging studies such as X‐rays, MRI, or CT. The current gold standard diagnostic modality is intra‐articular SIJ blocks using two differing local anesthetics. Current treatments for SIJ dysfunction may be beneficial for short‐term relief but lack long‐term efficacy. The purpose of our study was to examine the outcomes of patients who underwent minimally invasive, posterior SIJ fusion using allograft at a single center.MethodsThis was a retrospective study which received exemption from the WCG IRB. Data regarding preoperative and postoperative pain levels, surgical time, complications, and medication usage were obtained retrospectively from patient electronic medical records and prescription drug monitoring program reports. No mapping was completed prior to the procedure. Pain was assessed with the 11‐point (0–10) Visual Analogue Scale (VAS) and medication usage was assessed using Morphine Milligram Equivalents (MME). Patients were included if they had been diagnosed with SIJ dysfunction using two intra‐articular diagnostic blocks that resulted in at least an 80% decrease in pain and had failed conservative management. Patients with sacral insufficiency fractures were excluded.ResultsVAS scores reduced from 8.26 (SD = 1.09) at baseline to 2.59 (SD = 2.57), 2.55 (SD = 2.56), 2.71 (SD = 2.88), and 2.71 (SD = 2.88) at 3, 6, 9, and 12 months, respectively. MME reduced from 78.21 mg (SD = 51.33) to 58.95 mg (SD = 48.64), 57.61 mg (SD = 47.92), 61.71 mg (SD = 45.64), and 66.29 mg (SD = 51.65) at 3, 6, 9, and 12 months, respectively. All reductions in VAS scores and MME were statistically significant. No adverse events occurred, and the average operating room time was 40.16 min (SD = 6.27).ConclusionMinimally invasive, posterior SIJ fusion using allograft is a safe and efficacious method for managing SIJ dysfunction.
DEAD‐box RNA helicase RH20 positively regulates RNAi‐based antiviral immunity in plants by associating with SGS3/RDR6 bodies
Authors: Wen Zhiyan , Hu Rujian , Pi Qinglin , Zhang Dingliang , Duan Jiangning , Li Zhen , Li Qian , Zhao Xiaoyun , Yang Meng , Zhao Xiaofei , Liu Deshui , Su Zhen , Li Dawei , Zhang Yongliang 
Published: August 2024

SummaryRNA silencing plays a crucial role in defending against viral infections in diverse eukaryotic hosts. Despite extensive studies on core components of the antiviral RNAi pathway such as DCLs, AGOs and RDRs proteins, host factors involved in antiviral RNAi remain incompletely understood. In this study, we employed the proximity labelling approach to identify the host factors required for antiviral RNAi in Nicotiana benthamiana. Using the barley stripe mosaic virus (BSMV)‐encoded γb, a viral suppressor of RNA silencing (VSR), as the bait protein, we identified the DEAD‐box RNA helicase RH20, a broadly conserved protein in plants and animals with a homologous human protein known as DDX5. We demonstrated the interaction between RH20 and BSMV γb. Knockdown or knockout of RH20 attenuates the accumulation of viral small interfering RNAs, leading to increased susceptibility to BSMV, while overexpression of RH20 enhances resistance to BSMV, a process requiring the cytoplasmic localization and RNA‐binding activity of RH20. In addition to BSMV, RH20 also negatively regulates the infection of several other positive‐sense RNA viruses, suggesting the broad‐spectrum antiviral activity of RH20. Mechanistic analysis revealed the colocalization and interaction of RH20 with SGS3/RDR6, and disruption of either SGS3 or RDR6 undermines the antiviral function of RH20, suggesting RH20 as a new component of the SGS3/RDR6 bodies. As a counter‐defence, BSMV γb VSR subverts the RH20‐mediated antiviral defence by interfering with the RH20–SGS3 interaction. Our results uncover RH20 as a new positive regulator of antiviral RNAi and provide new potential targets for controlling plant viral diseases.
ORANGE interplays with TCP7 to regulate endoreduplication and leaf size
Authors: Wang Qi , Wang Linjuan , Song Shuyuan , Zhao Ya‐Nan , Gu Hong‐Hui , Zhu Ziqiang , Wang Jiansheng , Lu Shan 
Published: August 2024

SUMMARYLeaf size is a crucial agronomic trait directly affecting crop yield, which is mainly determined by coordinated cell proliferation, growth, and differentiation. Although endoreduplication is known to be correlated with the onset of cell differentiation and leaf size, the underlying molecular mechanisms are largely unclear. The DnaJ‐like zinc finger domain‐containing protein ORANGE (OR) was initially demonstrated to confer the massive accumulation of carotenoids in cauliflower curds. However, the cauliflower or mutant also possesses other phenotypes such as smaller curds, smaller leaves with elongated petioles, and delayed flowering. Here, we demonstrated that OR physically interacts with the transcription factor TCP7, which promotes endoreduplication by inducing the expression of the cell cycle gene CYCLIN D 1;1 (CYCD1;1). Overexpression of OR resulted in smaller rosette leaves, whereas the OR‐silencing plants had larger rosette leaves than wild‐type plants. Our microscopic observations and flow cytometry analysis revealed that the variation in leaf size was a result of different endoreduplication levels. Genetic analyses showed that OR functions antagonistically with TCP7 in regulating the endoreduplication levels in leaf cells. While the expression of OR is induced by TCP7, OR represses the transactivation activity of TCP7 by affecting its binding capability to the TCP‐binding motif in the promoter region of CYCD1;1. Through this interaction, OR negatively regulates the expression of CYCD1;1 and reduces the nuclear ploidy level in rosette leaf cells. Our findings provide new insights into the regulatory network of leaf size and also reveal a regulatory circuit controlling endoreduplication in leaf cells.
Muscle‐derived IL‐1β regulates EcSOD expression via the NBR1‐p62‐Nrf2 pathway in muscle during cancer cachexia
Authors: Yamada Mami , Warabi Eiji , Oishi Hisashi , Lira Vitor A. , Okutsu Mitsuharu 
Published: August 2024

AbstractOxidative stress contributes to the loss of skeletal muscle mass and function in cancer cachexia. However, this outcome may be mitigated by an improved endogenous antioxidant defence system. Here, using the well‐established oxidative stress‐inducing muscle atrophy model of Lewis lung carcinoma (LLC) in 13‐week‐old male C57BL/6J mice, we demonstrate that extracellular superoxide dismutase (EcSOD) levels increase in the cachexia‐prone extensor digitorum longus muscle. LLC transplantation significantly increased interleukin‐1β (IL‐1β) expression and release from extensor digitorum longus muscle fibres. Moreover, IL‐1β treatment of C2C12 myotubes increased NBR1, p62 phosphorylation at Ser351, Nrf2 nuclear translocation and EcSOD protein expression. Additional studies in vivo indicated that intramuscular IL‐1β injection is sufficient to stimulate EcSOD expression, which is prevented by muscle‐specific knockout of p62 and Nrf2 (i.e. in p62 skmKO and Nrf2 skmKO mice, respectively). Finally, since an increase in circulating IL‐1β may lead to unwanted outcomes, we demonstrate that targeting this pathway at p62 is sufficient to drive muscle EcSOD expression in an Nrf2‐dependent manner. In summary, cancer cachexia increases EcSOD expression in extensor digitorum longus muscle via muscle‐derived IL‐1β‐induced upregulation of p62 phosphorylation and Nrf2 activation. These findings provide further mechanistic evidence for the therapeutic potential of p62 and Nrf2 to mitigate cancer cachexia‐induced muscle atrophy.
imageKey points
Oxidative stress plays an important role in muscle atrophy during cancer cachexia.
EcSOD, which mitigates muscle loss during oxidative stress, is upregulated in 13‐week‐old male C57BL/6J mice of extensor digitorum longus muscles during cancer cachexia.
Using mouse and cellular models, we demonstrate that cancer cachexia promotes muscle EcSOD protein expression via muscle‐derived IL‐1β‐dependent stimulation of the NBR1‐p62‐Nrf2 signalling pathway.
These results provide further evidence for the potential therapeutic targeting of the NBR1‐p62‐Nrf2 signalling pathway downstream of IL‐1β to mitigate cancer cachexia‐induced muscle atrophy.
Determinants of maturation of the
Staphylococcus aureus
autoinducing peptide
Authors: Fang Liwei , Cosgriff Chance , Alonzo Francis 
Published: August 2024

ABSTRACT

The
a
ccessory
g
ene
r
egulatory (Agr) system is required for virulence factor gene expression and pathogenesis of
Staphylococcus aureus
. The Agr system is activated in response to the accumulation of a cyclic autoinducing peptide (AIP), which is matured and secreted by the bacterium. The precursor of AIP, AgrD, consists of the AIP flanked by an N-terminal

α

-helical Leader and a charged C-terminal tail. AgrD is matured to AIP by the action of two proteases, AgrB and MroQ. AgrB cleaves the C-terminal tail and promotes the formation of a thiolactone ring, whereas MroQ cleaves the N-terminal Leader in a manner that depends on the four-amino acid linker immediately following a conserved IG helix breaker motif. However, the attributes of AgrD that dictate the sequence of events in peptide maturation are not fully defined. Here, we used engineered AgrD peptide intermediates to ascertain the sufficiency of MroQ for N-terminal peptide cleavage, peptide export, and generation of mature AIP. We found that MroQ promotes the removal of the N-terminal Leader peptide from both linear and cyclic peptide intermediates, while peptide cyclization remained essential for signaling. The expression of the Leader peptide in isolation was sufficient for MroQ-dependent cleavage proximal to the four-amino-acid linker. In addition, active site mutations within AgrB destabilized full-length AgrD and thiolactone-containing intermediates and prevented the release of the Leader peptide. Altogether, our data support a tandem peptide maturation event involving both MroQ and AgrB that appears to couple protease activity and export of bioactive AIP.

IMPORTANCE

The
a
ccessory
g
ene
r
egulatory (Agr) system is important for
S. aureus
pathogenesis. Activation of the Agr system requires recognition of a cyclic peptide pheromone, which must be fully matured to exert its biological activity. The complete events in cyclic peptide maturation and export from the bacterial cell remain to be fully defined. We and others recently discovered that the membrane peptidase MroQ is required for pheromone maturation. This study builds off the identification of MroQ and considers the attributes of the pheromone pro-peptide that are required for MroQ-mediated processing as well as uncovers features important for peptide stability and export. Overall, the findings in this study have implications for understanding bacterial pheromone maturation and virulence.
Exploring the ergogenic potential of carbohydrate–caffeine combined mouth rinse on exercise and cognitive performance: a systematic review
Authors: Poon Eric Tsz-Chun , Tsang Jaclyn Hei , Sun Fenghua , Ali Ajmol , Rollo Ian , Wong Stephen Heung-Sang 
Published: August 2024

Carbohydrate (CHO) and caffeine (CAF) mouth rinsing have been independently reported to benefit sport performance. The proposed mechanisms by which mouth rinsing CHO exerts an influence are reported to be different from those for mouth rinsing CAF. However, the potential ergogenic effects of combining CHO and CAF in a single mouth rinse solution, are unclear. This study aimed to review the available evidence of CHO–CAF combined mouth rinse on exercise and cognitive performance in human participants. A systematic literature search was conducted using five databases until April 2024, following the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) recommendations. Among the nine randomized crossover studies included, only one study showed significant improvements in lower-body muscular endurance with CHO–CAF mouth rinse (effect size (ES): 0.48; p < 0.05), while two studies reported non-statistically significant improvements in repeated sprint performance compared to other mouth rinse and placebo conditions (ES: 0.20–0.81; p = 0.07–0.18). However, for other performance measures, including repeated jumps, upper-body strength and endurance, endurance cycling, and intermittent recovery run, most evidence (five studies) did not demonstrate significant ergogenic effects. Notably, of the two studies that examined cognitive performance, both reported significant improvements with CHO–CAF mouth rinse compared with the placebo condition (ES: 0.45–3.45; p < 0.05). Overall, a synergistic influence of CHO–CAF mouth rinse on physical exercise performance is not evident, but preliminary evidence suggests potential benefits on cognitive performance. Future studies are required to address various methodological issues identified in this review, while practitioners and athletes should exercise caution when considering this novel nutritional strategy.
Relative injective modules, superstability and noetherian categories
Authors: Mazari-Armida Marcos , Rosický Jiří 
Published: August 2024

We study classes of modules closed under direct sums, [Formula: see text]-submodules and [Formula: see text]-epimorphic images where [Formula: see text] is either the class of embeddings, RD-embeddings or pure embeddings. We show that the [Formula: see text]-injective modules of theses classes satisfy a Baer-like criterion. In particular, injective modules, RD-injective modules, pure injective modules, flat cotorsion modules and [Formula: see text]-torsion pure injective modules satisfy this criterion. The argument presented is a model theoretic one. We use in an essential way stable independence relations which generalize Shelah’s non-forking to abstract elementary classes. We show that the classical model theoretic notion of superstability is equivalent to the algebraic notion of a noetherian category for these classes. We use this equivalence to characterize noetherian rings, pure semisimple rings, perfect rings and finite products of finite rings and artinian valuation rings via superstability.
My Fuzzers Won’t Build: An Empirical Study of Fuzzing Build Failures
Authors: Nourry Olivier , Kashiwa Yutaro , Shang Weiyi , Shu Honglin , Kamei Yasutaka 
Published: August 2024

Fuzzing is an automated software testing technique used to find software vulnerabilities that works by sending large amounts of inputs to a software system to trigger bad behaviors. In recent years, the open source software ecosystem has seen a significant increase in the adoption of fuzzing to avoid spreading vulnerabilities throughout the ecosystem. While fuzzing can uncover vulnerabilities, there is currently a lack of knowledge regarding the challenges of conducting fuzzing activities over time. Specifically, fuzzers are very complex tools to set up and build before they can be used.
We set out to empirically find out how challenging is build maintenance in the context of fuzzing. We mine over 1.2 million build logs from Google’s OSS-Fuzz service to investigate fuzzing build failures. We first conduct a quantitative analysis to quantify the prevalence of fuzzing build failures. We then manually investigate 677 failing fuzzing builds logs and establish a taxonomy of 25 root causes of build failures. We finally train a machine learning model to recognize common failure patterns in failing build logs. Our taxonomy can serve as a reference for practitioners conducting fuzzing build maintenance. Our modeling experiment shows the potential of using automation to simplify the process of fuzzing.