bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-term changes in adiposity markers during and after antidepressant therapy in a community cohort
Authors: Mwinyi Jessica , Strippoli Marie-Pierre F. , Kanders Sofia H. , Schiöth Helgi B. , Eap Chin B. , Lasserre Aurélie M. , Marques-Vidal Pedro , Vandeleur Caroline L. , Preisig Martin 
Published: August 2024

AbstractResearch on antidepressant-related weight changes over more than 12 months is scarce and adjustment for the effects of depressive episodes has rarely been applied. Accordingly, our aim was to assess the associations of the use of any antidepressants, subclasses of antidepressant and specific compounds prior to baseline and during a 5.5-year follow-up with changes in adiposity markers, and the effect of sex on these associations, with adjustment for multiple confounders including the effects of depressive episodes and their severity. Data stemmed from a prospective cohort study including 2479 randomly selected 35–66 year-old residents of an urban area (mean age 49.9 years, 53.3% women) who underwent physical and psychiatric evaluations at baseline and follow-up. Weight, height, waist circumference, and body fat were measured by trained nurses and information on diagnosis and antidepressant use prior to baseline and during follow-up was collected through standardized interviews. In the fully adjusted models, the number of antidepressants, mainly SSRIs and TCAs, used prior to baseline, was associated with a lower increase of body-mass index (BMI, β (95%CI) = −0.12 (−0.19, −0.05)) and waist circumference (β = −0.28 (−0.56, −0.01)), whereas participants treated with antidepressants during the follow-up had a steeper increase in BMI (β = 0.32 (0.13, 0.50)) and waist circumference (β = 1.23 (0.44, 2.01)). Within the class of SSRIs, the use of fluoxetine, sertraline or escitalopram during follow-up was associated with a steeper increase in adiposity markers. The associations of SSRIs with BMI and waist circumference were only observed when the SSRIs were used during the second period of the follow-up. Sex did not moderate these associations. Our findings suggest an increase of adiposity markers during sustained treatment with TCAs and SSRIs, which however return to normal levels after cessation of treatment. Hence, the benefit of long-term administration of these antidepressants should be carefully weighed against the potential risk of weight gain.
Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib
Authors: Feng Yu , Luo Hongmei , Huang Jingcao , Zhang Yue , Wen Jingjing , Li Linfeng , Mi Ziyue , Gao Qianwen , He Siyao , Liu Xiang , Zhai Xinyu , Wang Xin , Zhang Li , Niu Ting , Zheng Yuhuan 
Published: August 2024

AbstractProteasome inhibitors (PIs), such as bortezomib and calfizomib, were backbone agents in the treatment of multiple myeloma (MM). In this study, we investigated bortezomib interactors in MM cells and identified dihydrolipoamide dehydrogenase (DLD) as a molecular target of bortezomib. DLD catalyzes the oxidation of dihydrolipoamide to form lipoamide, a reaction that also generates NADH. Our data showed that bortezomib bound to DLD and inhibited DLD’s enzymatic function in MM cells. DLD knocked down MM cells (DLD-KD) had decreased levels of NADH. Reduced NADH suppressed assembly of proteasome complex in cells. As a result, DLD-KD MM cells had decreased basal-level proteasome activity and were more sensitive to bortezomib. Since PIs were used in many anti-MM regimens in clinics, we found that high expression of DLD correlated with inferior prognosis of MM. Considering the regulatory role of DLD in proteasome assembly, we evaluated DLD targeting therapy in MM cells. DLD inhibitor CPI-613 showed a synergistic anti-MM effect with bortezomib in vitro and in vivo. Overall, our findings elucidated DLD as an alternative molecular target of bortezomib in MM. DLD-targeting might increase MM sensitivity to PIs.
Sulfur starvation-induced autophagy in Saccharomyces cerevisiae involves SAM-dependent signaling and transcription activator Met4
Authors: Prigent Magali , Jean-Jacques Hélène , Naquin Delphine , Chédin Stéphane , Cuif Marie-Hélène , Legouis Renaud , Kuras Laurent 
Published: August 2024

AbstractAutophagy is a key lysosomal degradative mechanism allowing a prosurvival response to stresses, especially nutrient starvation. Here we investigate the mechanism of autophagy induction in response to sulfur starvation in Saccharomyces cerevisiae. We found that sulfur deprivation leads to rapid and widespread transcriptional induction of autophagy-related (ATG) genes in ways not seen under nitrogen starvation. This distinctive response depends mainly on the transcription activator of sulfur metabolism Met4. Consistently, Met4 is essential for autophagy under sulfur starvation. Depletion of either cysteine, methionine or SAM induces autophagy flux. However, only SAM depletion can trigger strong transcriptional induction of ATG genes and a fully functional autophagic response. Furthermore, combined inactivation of Met4 and Atg1 causes a dramatic decrease in cell survival under sulfur starvation, highlighting the interplay between sulfur metabolism and autophagy to maintain cell viability. Thus, we describe a pathway of sulfur starvation-induced autophagy depending on Met4 and involving SAM as signaling sulfur metabolite.
Lack of detectable HPV18 antibodies in 14% of quadrivalent vaccinees in a longitudinal cohort study
Authors: Gray Penelope , Mariz Filipe Colaço , Eklund Carina , Eriksson Tiina , Faust Helena , Kann Hanna , Müller Martin , Paavonen Jorma , Pimenoff Ville N. , Sehr Peter , Surcel Heljä-Marja , Dillner Joakim , Waterboer Tim , Lehtinen Matti 
Published: August 2024

AbstractAlthough HPV vaccines are highly efficacious, a notable proportion of quadrivalent vaccinees are HPV18 seronegative post-vaccination. We have investigated this findings’ validity by comparing vaccine-induced antibody responses using two different immunoassays. 6558 16–17-year-old females participated in the FUTURE II (NCT00092534) and PATRICIA (NCT00122681) trials in 2002–2004. Both the quadrivalent and bivalent vaccine recipients (QVR and BVR) received three doses. Twelve-year follow-up for 648 vaccinees was conducted by the Finnish Maternity Cohort. The presence of neutralising and binding HPV antibodies was analysed via HPV pseudovirion-based neutralisation and pseudovirion-binding assays. Four percent and 14.3% of the QVRs were seronegative for neutralising and binding antibodies to HPV16 and HPV18, respectively. No BVRs were HPV16/18 seronegative post-vaccination. The antibody titres were strongly correlated between the assays, Pearson’s correlation coefficient, r[HPV16] = 0.92 and 0.85, and r[HPV18] = 0.91 and 0.86 among the QVRs and BVRs respectively. Fourteen percent of QVRs lacked detectable HPV18 antibodies in long-term follow-up.
EStreams: An integrated dataset and catalogue of streamflow, hydro-climatic and landscape variables for Europe
Authors: do Nascimento Thiago V. M. , Rudlang Julia , Höge Marvin , van der Ent Ruud , Chappon Máté , Seibert Jan , Hrachowitz Markus , Fenicia Fabrizio 
Published: August 2024

AbstractLarge-sample hydrology datasets have become increasingly available, contributing to significant scientific advances. However, in Europe, only a few such datasets have been published, capturing only a fraction of the wealth of information from national data providers in terms of available spatial density and temporal extent. We present “EStreams”, an extensive dataset of hydro-climatic variables and landscape descriptors and a catalogue of openly available stream records for 17,130 European catchments. Spanning up to 120 years, the dataset includes streamflow indices, catchment-aggregated hydro-climatic signatures and landscape attributes (topography, soils, geology, vegetation and landcover). The catalogue provides detailed descriptions that allow users to directly access streamflow data sources, overcoming challenges related to data redistribution policies, language barriers and varied data portal structures. EStreams also provides Python scripts for data retrieval, aggregation and processing, making it dynamic in contrast to static datasets. This approach enables users to update their data as new records become available. Our goal is to extend current large-sample datasets and further integrate hydro-climatic and landscape data across Europe.
JR5558 mice are a reliable model to investigate subretinal fibrosis
Authors: Seyed-Razavi Yashar , Lee So-Ra , Fan Jiawen , Shen Weiyong , Cornish Elisa E. , Gillies Mark C. 
Published: August 2024

AbstractSubretinal fibrosis is a major untreatable cause of poor outcomes in neovascular age-related macular degeneration. Mouse models of subretinal fibrosis all possess a degree of invasiveness and tissue damage not typical of fibrosis progression. This project characterises JR5558 mice as a model to study subretinal fibrosis. Fundus and optical coherence tomography (OCT) imaging was used to non-invasively track lesions. Lesion number and area were quantified with ImageJ. Retinal sections, wholemounts and Western blots were used to characterise alterations. Subretinal lesions expand between 4 and 8 weeks and become established in size and location around 12 weeks. Subretinal lesions were confirmed to be fibrotic, including various cell populations involved in fibrosis development. Müller cell processes extended from superficial retina into subretinal lesions at 8 weeks. Western blotting revealed increases in fibronectin (4 wk and 8 wk, p < 0.001), CTGF (20 wks, p < 0.001), MMP2 (12 wks and 20 wks p < 0.05), αSMA (12 wks and 20 wks p < 0.05) and GFAP (8 wk and 12 wk, p ≤ 0.01), consistent with our immunofluorescence results. Intravitreal injection of Aflibercept reduced subretinal lesion growth. Our study provides evidence JR5558 mice have subretinal fibrotic lesions that grow between 4 and 8 weeks and confirms this line to be a good model to study subretinal fibrosis development and assess treatment options.
Bioactivity of silverleaf nightshade (Solanum elaeagnifolium Cav.) berries parts against Galleria mellonella and Erwinia carotovora and LC-MS chemical profile of its potential extract
Authors: Kobisi Abdel Nasser A. , Balah Mohamed A. , Hassan Ahmed R. 
Published: August 2024

AbstractNatural products received much attention as an environmentally beneficial solution for pest management. Therefore, the extracts of invasive silverleaf nightshade (Solanum elaeagnifolium Cav.) weeds using their berries parts (seeds, peels and mucilage) supported by bioassay-guided fractionation were tested against both the greater wax moth (Galleria mellonella) and Erwinia carotovora pv. carotovora causes of the blackleg of potatoes. The seeds and peels of S. elaeagnifolium were successively extracted by maceration using dichloromethane (DCM), ethyl acetate (EtOAc), and ethanol (EtOH), respectively. While, its mucilage was extracted using EtOAc. The successive EtOH extract of the plant seeds had promising inhibition efficacy and the best minimal inhibition concentration (MIC) of 50 µg/ml against E. Carotovora amongst other extracts (DCM and EtOAc of the plant berries parts). Depending on dose response activity, EtOH extract had G. mellonella larval mortality and pupal duration rates (LC50; 198.30 and LC95; 1294.73 µg/ml), respectively. Additionally, this EtOH extract of seeds was fractionated using preparative TLC to three characteristic bands. The insecticidal and bacterial activities of these isolated bands (SEA, SEB, and SEC) were evaluated at a dose of 100 µg/ml, causing mortality by 48.48, 62.63 and 92.93% (G. mellonella larvae) and inhibition by 15.22, 0.00 and 31.66 mm (E. carotovora), respectively. Moreover, the separated major three bands were tentatively identified using LC-ESI-MS analysis revealing the presence of two phenolic acids; chlorogenic acid (SEA) and dicaffeoyl quinic acid (SEB) in addition to one steroidal saponin (SEC) annotated as borassoside E or yamoscin. Finally, the plant seeds’ successive EtOH extract as well as its active constituents, exhibited potential broad-spectrum activity and the ability to participate in future pest management initiatives. A field study is also recommended to validate its bio-efficacy against selected pests and to develop its formulations.
Unified framework for laser-induced transient bubble dynamics within microchannels
Authors: Nagalingam Nagaraj , Korede Vikram , Irimia Daniel , Westerweel Jerry , Padding Johan T. , Hartkamp Remco , Eral Hüseyin Burak 
Published: August 2024

AbstractOscillatory flow in confined spaces is central to understanding physiological flows and rational design of synthetic periodic-actuation based micromachines. Using theory and experiments on oscillating flows generated through a laser-induced cavitation bubble, we associate the dynamic bubble size (fluid velocity) and bubble lifetime to the laser energy supplied—a control parameter in experiments. Employing different channel cross-section shapes, sizes and lengths, we demonstrate the characteristic scales for velocity, time and energy to depend solely on the channel geometry. Contrary to the generally assumed absence of instability in low Reynolds number flows ($$<1000$$

<
1000

), we report a momentary flow distortion that originates due to the boundary layer separation near channel walls during flow deceleration. The emergence of distorted laminar states is characterized using two stages. First the conditions for the onset of instabilities is analyzed using the Reynolds number and Womersley number for oscillating flows. Second the growth and the ability of an instability to prevail is analyzed using the convective time scale of the flow. Our findings inform rational design of microsystems leveraging pulsatile flows via cavitation-powered microactuation.
Association of information technology ability, workplace social engagement, and successful ageing: validation of a short measure with three African samples
Authors: Asiamah Nestor , Hatsu Sylvester , Muhonja Faith , Opara Confidence Chinwe , Opuni Frank Frimpong , Danquah Emelia , Sghaier Sarra 
Published: August 2024

AbstractThis study examined the association of workplace social engagement (WSE) and information technology ability (ITA) with successful ageing and validated a brief scale measuring WSE. The interaction of WSE and ITA on successful ageing was also assessed. A cross-sectional design was adopted, and the participants were 1186 older adults living in Kenya (n = 350), Nigeria (n = 260), and Ghana (n = 576). Pearson’s correlation and factor analyses of two datasets (i.e., waves 1 and 2) from the sample were utilised to validate the WSE scale. Hierarchical linear regression analyses with relevant sensitivity analyses were utilised to assess the associations with wave 2 data. The WSE scale produced satisfactory psychometric properties (i.e., reliability and validity) as a unidimensional measure. WSE and ITA were positively associated with successful ageing in Kenya and Ghana and in the consolidated data. The interaction between WSE and ITA was positively associated with successful ageing and its domains (i.e., illness avoidance, functioning, and engagement with life) in Kenya, Ghana, and consolidated data. At higher ITA or the use of information technologies, WSE is less strongly associated with successful ageing. WSE is more strongly associated with successful ageing only at moderate ITA.
The high-pressure structure of (1-x)Na$$_{0.5}$$Bi$$_{0.5}$$TiO$$_3$$-xBaTiO$$_3$$ at the morphotropic phase boundary
Authors: Rösche Constanze , Boffa Ballaran Tiziana , Malcherek Thomas , Paulmann Carsten , Angel Ross John , Gorfman Semën , Mihailova Boriana 
Published: August 2024

AbstractThe pressure-induced structural changes in the perovskite-type (ABO$$_3$$

3

) ferroelectric solid solution (1-x)Na$$_{0.5}$$

0.5

Bi$$_{0.5}$$

0.5

TiO$$_3$$

3

-xBaTiO$$_3$$

3

(NBT-xBT) at the morphotropic phase boundary (MPB) ($$x_{\text {MPB}}=0.048$$

x
MPB

=
0.048

) have been analyzed up to 12.3 GPa by single-crystal x-ray diffraction with synchrotron radiation. A pressure-induced phase transition takes place between 4.4 and 5.0 GPa, where the pseudocubic low-pressure phase transforms into an orthorhombic high-pressure phase with space group Pnma. The high-pressure phase is comprised of mixed BO$$_6$$

6

tilts and anti-polar A-cation displacements, without exhibiting coherent off-centered shifts of the B-site Ti$$^{4+}$$

4
+

cations that can be detected by synchrotron x-ray diffraction. Our results reveal that at ambient pressure and room temperature the NBT-$$x_\mathrm{{MPB}}$$

x
MPB

BT structure possesses anti-phase BO$$_6$$

6

tilts with a relatively large correlation length and the same type of polar distortions as those present in pure NBT, but with strongly violated correlation length due to Ba$$^{2+}$$

2
+

-induced local elastic-stress fields. For $$x_{\text {MPB}}$$

x
MPB

the effect of Ba on the mesoscopic-scale structure is compensated by a mild external pressure of only 0.7 GPa, resulting in structural features resembling those of pure NBT at ambient conditions.
Chloroplast genomes of Eriobotrya elliptica and an unknown wild loquat “YN-1”
Authors: Lin Zhicong , Guo Qing , Ma Shiwei , Lin Hailan , Lin Shunquan , Lin Shoukai , Wu Jincheng 
Published: August 2024

AbstractThe chloroplast genomes of wild loquat can help to determine their place in the history of evolution. Here, we sequenced and assembled two novel wild loquat’s chloroplast genomes, one is Eriobotrya elliptica, and the other is an unidentified wild loquat, which we named “YN-1”. Their sizes are 159,471 bp and 159,399 bp, respectively. We also assembled a cultivated loquat named ‘JFZ’, its chloroplast genome size is 159,156 bp. A comparative study was conducted with six distinct species of loquats, including five wild loquats and one cultivated loquat. The results showed that both E. elliptica and “YN-1” have 127 genes, one gene more than E. fragrans, which is psbK. Regions trnF-GAA-ndhJ, petG-trnP-UGG, and rpl32-trnL-UAG were found to exhibit high variability. It was discovered that there was a positive selection on rpl22 and rps12. RNA editing analysis found several chilling stress-specific RNA editing sites, especially in rpl2 gene. Phylogenetic analysis results showed that “YN-1” is closely related to E. elliptica, E. obovata and E. henryi.
Hepatic stellate cells in zone 1 engage in capillarization rather than myofibroblast formation in murine liver fibrosis
Authors: Khan Muhammad Ashfaq , Fischer Julian , Harrer Leon , Schwiering Fabian , Groneberg Dieter , Friebe Andreas 
Published: August 2024

AbstractThe combination of lineage tracing and immunohistochemistry has helped to identify subpopulations and fate of hepatic stellate cells (HSC) in murine liver. HSC are sinusoidal pericytes that act as myofibroblast precursors after liver injury. Single cell RNA sequencing approaches have recently helped to differentiate central and portal HSC. A specific Cre line to lineage trace portal HSC has not yet been described. We used three Cre lines (Lrat-Cre, PDGFRβ-CreERT2 and SMMHC-CreERT2) known to label mesenchymal cells including HSC in combination with a tdTomato-expressing reporter. All three Cre lines labeled populations of HSC as well as smooth muscle cells (SMC). Using the SMMHC-CreERT2, we identified a subtype of HSC in the periportal area of the hepatic lobule (termed zone 1-HSC). We lineage traced tdTomato-expressing zone 1-HSC over 1 year, described fibrotic behavior in two fibrosis models and investigated their possible role during fibrosis. This HSC subtype resides in zone 1 under healthy conditions; however, zonation is disrupted in preclinical models of liver fibrosis (CCl4 and MASH). Zone 1-HSC do not transform into αSMA-expressing myofibroblasts. Rather, they participate in sinusoidal capillarization. We describe a novel subtype of HSC restricted to zone 1 under physiological conditions and its possible function after liver injury. In contrast to the accepted notion, this HSC subtype does not transform into αSMA-positive myofibroblasts; rather, zone 1-HSC adopt properties of capillary pericytes, thereby participating in sinusoidal capillarization.
Activity of botulinum neurotoxin X and its structure when shielded by a non-toxic non-hemagglutinin protein
Authors: Martínez-Carranza Markel , Škerlová Jana , Lee Pyung-Gang , Zhang Jie , Krč Ajda , Sirohiwal Abhishek , Burgin Dave , Elliott Mark , Philippe Jules , Donald Sarah , Hornby Fraser , Henriksson Linda , Masuyer Geoffrey , Kaila Ville R. I. , Beard Matthew , Dong Min , Stenmark Pål 
Published: August 2024

AbstractBotulinum neurotoxins (BoNTs) are the most potent toxins known and are used to treat an increasing number of medical disorders. All BoNTs are naturally co-expressed with a protective partner protein (NTNH) with which they form a 300 kDa complex, to resist acidic and proteolytic attack from the digestive tract. We have previously identified a new botulinum neurotoxin serotype, BoNT/X, that has unique and therapeutically attractive properties. We present the cryo-EM structure of the BoNT/X-NTNH/X complex and the crystal structure of the isolated NTNH protein. Unexpectedly, the BoNT/X complex is stable and protease-resistant at both neutral and acidic pH and disassembles only in alkaline conditions. Using the stabilizing effect of NTNH, we isolated BoNT/X and showed that it has very low potency both in vitro and in vivo. Given the high catalytic activity and translocation efficacy of BoNT/X, low activity of the full toxin is likely due to the receptor-binding domain, which presents very weak ganglioside binding and exposed hydrophobic surfaces.
Data‐driven Buck converter model identification method with missing outputs
Authors: Hou Jie , Zhang Xinhua , Wang Huiming , Wang Shiwei 
Published: August 2024

AbstractA data‐driven Buck converter model identification method is proposed to deal with missing (incomplete) outputs, which is robust to the data length and percentage of missing data. A nuclear norm based convex optimization problem instead of linear interpolation, to guarantee the recovered missing data satisfying the potential model structured low‐rank character, is constructed to estimate missing outputs. The alternating direction method of multiplier strategy is used to solve the nuclear norm based convex optimization problem. In this way, the high‐quality missing data can be estimated, even for short data length and high percentage of missing data. Based on the recovered data, the subspace identification method provides accurate estimates of the structure and parameter of the Buck converter synchronously. By applying the proposed method to a Buck converter, experimental results demonstrate its effectiveness.