bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross‐talk of alpha tocopherol‐associated protein and JNK controls the oxidative stress‐induced apoptosis in prostate cancer cells
Authors: Zhu Baoyi , Li Xiaojuan , Zhang Yuying , Ye Chunwei , Wang Yu , Cai Songwang , Huang Huaiqiu , Cai Yi , Yeh Shuyuan , Huang Zhenhua , Chen Ruihan , Tao Yiran , Wen Xingqiao 
Published: November 2012

AbstractExcess intracellular reactive oxygen species (ROS) beyond a threshold can induce apoptosis in cancer cells. However, the signal pathways that can augment the proapoptotic function of ROS remain largely unknown. We previously identified a tumor suppressor, alpha‐tocopherol‐associated protein (TAP), yet little is known regarding the role of TAP in the apoptotic signaling in prostate cancer. Interestingly, we recently found that exposure of prostate cancer cells to hydrogen peroxide (H2O2) resulted in induced apoptosis as well as increased expression of TAP. Small interfering RNA (siRNA) mediated silencing of endogenous TAP expression conferred effective protection from H2O2‐induced apoptosis. Further mechanistic study showed exposure of prostate cancer cells to H2O2 resulted in increased phosphorylation of both JNK and c‐Jun, and TAP siRNA effectively decreased H2O2‐induced JNK and c‐Jun phosphorylation. Immunoprecipitation experiments revealed that JNK physically associates with TAP. Furthermore, signaling downstream of JNK to the AP‐1 complex and BH‐3‐only subfamily were found to be regulated on changing the TAP expression status. TAP could also promote the oxidative stress‐induced apoptosis effect of docetaxel. In the mice xenograft model, H2O2 treatment induced TAP expression, JNK phosphorylation and apoptosis of prostate cancer. Recombinant adeno‐associated virus 2 (rAAV2)‐TAP injection significantly sensitizes this H2O2 proapoptotic effect. Together, we have identified a novel functional mechanism that the cross‐talk of TAP‐JNK is involved in oxidative stress‐induced apoptosis in prostate cancer cells. Disrupting the redox balance of cancer cells by this signaling may enable therapeutic selectivity and provide benefit to overcome the drug resistance of prostate cancer.
Peroxisome proliferator‐activated receptor‐γ cofactors in neurodegeneration
Authors: Katsouri Loukia , Blondrath Katrin , Sastre Magdalena 
Published: November 2012

AbstractPeroxisome proliferator‐activated receptor‐γ (PPARγ) was initially involved in the regulation of glucose and lipid metabolism, cell differentiation, as well as in the transcriptional control of a wide range of inflammatory genes. However, during the last decade, there has been evidence of the implication of this nuclear receptor in neurodegeneration. Various studies have shown that the administration of PPARγ ligands leads to a reduced pathology in many neurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, multiple sclerosis, Huntington's disease, and stroke. PPARγ cofactors have a critical function in regulating the activity of PPARγ. Recent reports have brought to light the role of the PPARγ coactivator‐1α (PGC‐1α) in several neurodegenerative pathologies. However, very little is know about other PPARγ cofactors in the brain, such as the receptor‐interacting protein 140, as well as the nuclear receptor corepressor, which seems to be required for normal neural development at specific embryonic stages. In this review, we aim to analyze the role of the main regulators of PPARγ in the brain and during neurodegeneration. © 2012 IUBMB IUBMB Life, 64(12): 958–964, 2012
On the moderating role of age in the relationship between pain and cognition
Authors: Oosterman J.M. , Gibson S.J. , Pulles W.L.J.A. , Veldhuijzen D.S. 
Published: November 2012

AbstractBackgroundA relationship between pain perception and cognitive function is evident. However, the directionality of this association is unclear and may be influenced by age. That is, inverse associations between pain and cognition have been reported in young and middle‐aged chronic pain patients, whereas higher clinical pain ratings have been associated with better cognitive performance in older chronic pain patients. Therefore, this study examined the possible moderating role of age in the pain‐cognition relationship.MethodTwenty‐two younger and 24 older chronic pain participants completed neuropsychological tests of psychomotor speed, memory and executive function. They also completed the McGill Pain Questionnaire to evaluate clinical pain.ResultsInteraction analyses revealed that age indeed moderates the relationship between clinical pain ratings and cognitive functions. In the younger age group, pain ratings were inversely related to memory and executive function. In the older age group, a positive relationship was found between pain ratings and executive function, whereas the inverse association of clinical pain with memory was no longer present.ConclusionsThis study was the first to confirm the hypothesis that age is an important moderator of the relationship between pain and cognition. An important finding is that in older adults, most inverse effects of pain on cognition are either no longer present or may even be reversed. The positive relationship between pain and executive function may indicate age‐related reduced integrity of a shared underlying neural substrate.
Neutrophils recruited by CXCR1/2 signalling mediate post‐incisional pain
Authors: Carreira E.U. , Carregaro V. , Teixeira M.M. , Moriconi A. , Aramini A. , Verri W.A. , Ferreira S.H. , Cunha F.Q. , Cunha T.M. 
Published: November 2012

AbstractBackgroundNeutrophil recruitment mediated by the CXCL1/KC chemokine and its receptors CXCR1/CXCR2 plays a critical role in inflammatory diseases. Recently, neutrophil migration and activation triggered by CXCL1‐CXCR1/2 signalling was implicated in inflammatory nociception; however, their role in post‐surgical pain has not been elucidated. In this study, we addressed the function of neutrophils in the genesis of post‐incisional pain in an experimental model of post‐surgical pain.MethodsMechanical hyperalgesia was determined with an electronic von Frey test in a mouse hindpaw incisional model. Neutrophil accumulation and the level of CXCL1/KC in the plantar tissue were determined by myeloperoxidase activity assay and enzyme‐linked immunosorbent assay, respectively.ResultsAn incision in the mouse hindpaw produces long‐lasting mechanical hyperalgesia that persists for at least 72 h after surgery. Following surgery, there was an increase in both neutrophil accumulation and the CXCL1/KC level in the incised paws. The depletion of the mouse neutrophils by vinblastine sulphate or anti‐neutrophil antibody treatments reduced the mechanical hyperalgesia after paw incision. Furthermore, the treatment of mice with ladarixin, an orally acting CXCR1/2 antagonist, also reduced both the mechanical hyperalgesia and the infiltration of neutrophils in the incised paws.ConclusionIn conclusion, it appears that after surgical processes, neutrophils are recruited by CXCL1‐CXCR1/2 signalling and participate in the cascade of events, leading to mechanical hyperalgesia. These results suggest that blocking neutrophil migration through the inhibition of CXCL1‐CXCR1/2 signalling might be a target to control post‐surgical pain.
Creating Spaces for Black Adolescent Girls to “Write It Out!”
Authors: Muhammad Gholnecsar E. 
Published: November 2012

AbstractIdentity and literacy development are two critical processes shaping the life trajectories of adolescents. Identity development in particular can present unique issues for Black adolescent girls, who are positioned in ways to negotiate their identity(ies) when presented with hegemonic language and representations of what is beauty and what is “Black.” Writing becomes a key literacy practice for Black adolescent girls to make meaning of their identity(ies). Although there are a growing number of studies on literacy and identity development of Black adolescent girls, few studies address writing. A single case study is conducted with a Black adolescent girl who struggles with selfhood to explore how she makes meaning of her identity through writing. She discusses writing within a Black adolescent female summer writing institute juxtaposed with school classrooms. Writing within the summer institute afforded opportunities for her to self‐exert selfhood, while in‐school writing assignments offer little opportunities for self‐expression.
Tensions in Teaching Adolescence/ts: Analyzing Resistances In A Young Adult Literature Course
Authors: Sarigianides Sophia Tatiana 
Published: November 2012

AbstractIdentifying English Education courses focused on young adult literature as apposite sites for exploring teacher conceptions of youth and the texts aimed for youths’ consumptions, this article addresses the multiple sources of tension—and pedagogical potential—of teaching a young adult literature course centrally framed around controversial discourses of adolescence. Reflecting on six years of teaching the course at two different institutions with a strong focus on critical perspectives of youth, the article examines why students in the class would understandably strain against this curriculum, as well as what it might mean for secondary literacy curricula and secondary students if teacher educators and secondary teachers take up these pedagogical risks and sources of resistance in efforts to interrupt dominant and stereotypical discourses about youth.
Developmental disorders and altered gene expression in the tropical clawed frog (Silurana tropicalis) exposed to 17α‐ethinylestradiol
Authors: Hirakawa Ikumi , Miyagawa Shinichi , Mitsui Naoko , Miyahara Maki , Onishi Yuta , Kagami Yoshihiro , Kusano Teruhiko , Takeuchi Takashi , Ohta Yasuhiko , Iguchi Taisen 
Published: November 2012

ABSTRACTSeveral endocrine‐disrupting chemicals with estrogenic activity can affect sexual development and reproduction in aquatic wildlife. The occurrence of oocytes in the testis (testis‐ova) is one reproductive disorder and can be used as a valid endpoint when studying disruptive effects of estrogenic chemicals. To elucidate the molecular basis of testis‐ova induction, we conducted gene expression analysis in the gonads of Silurana tropicalis exposed to 0, 3, 10 and 30 ng l−1 17α‐ethinylestradiol (EE2) from 2 days after fertilization to the juvenile stage (14 weeks after fertilization). The frequencies of testis‐ova induction or male to female sex‐reversal of the gonads increased in an EE2 dose‐dependent manner. Microarray analysis showed that expressions of a large number of genes were significantly changed by EE2 exposure. Genes including egg envelope composition (zp4, zpax, zpc, zp3.2 and egg cortical granule lectin), 42S particle genes (42Sp50, 42Sp43 and 42Sp48) and regulation of female germ cells (figla) are associated with the testis‐ova and sex‐reversal situation in the gonads. Of those, expression of zpc and 42Sp50 genes is associated with testis‐ova. Thus, we propose that these genes are useful biomarkers for toxicological research in amphibians developmentally exposed to estrogenic chemicals. Copyright © 2012 John Wiley & Sons, Ltd.
Differential Effect of DDT, DDE, and DDD on COX‐2 Expression in the Human Trophoblast Derived HTR‐8/SVneo Cells
Authors: Dominguez‐Lopez Pablo , Diaz‐Cueto Laura , Olivares Aleida , Ulloa‐Aguirre Alfredo , Arechavaleta‐Velasco Fabian 
Published: November 2012

ABSTRACTThe purpose of this study was to investigate the effect of 1,1,1‐trichloro‐2,2‐bis‐(chlorophenyl)ethane (DDT), 1,1‐bis‐(chlorophenyl)‐2,2‐dichloroethene (DDE), and 1,1‐dichloro‐2,2‐bis(chlorophenyl)ethane (DDD) isomers on COX‐2 expression in a human trophoblast‐derived cell line. Cultured HTR‐8/SVneo trophoblast cells were exposed to DDT isomers and its metabolites for 24 h, and COX‐2 mRNA and protein expression were assessed by RT‐PCR, Western blotting, and ELISA. Prostaglandin E2 production was also measured by ELISA. Both COX‐2 mRNA and protein were detected under control (unexposed) conditions in the HTR‐8/SVneo cell line. COX‐2 protein expression and prostaglandin E2 production but not COX‐2 mRNA levels increased only after DDE and DDD isomers exposure. It is concluded that DDE and DDD exposure induce the expression of COX‐2 protein, leading to increased prostaglandin E2 production. Interestingly, the regulation of COX‐2 by these organochlorines pesticides appears to be at the translational level. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:454‐460, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21444
Modulatory Effects of Curcumin and Green Tea Extract against Experimentally Induced Pulmonary Fibrosis: A Comparison with N‐Acetyl Cysteine
Authors: Hamdy Mohammed Ahmed , El‐Maraghy Shohda A. , Kortam Mona Abd El Aziz 
Published: November 2012

ABSTRACTThe study was aimed to investigate the protective effect of green tea extract (GTE), curcumin, and N‐acetyl cysteine (NAC) on experimentally induced pulmonary fibrosis. Curcumin (200 mg/kg b.w), GTE (150 mg/kg b.w), and NAC (490 mg/kg b.w) were administered orally for 14 days with concomitant administration of cyclophosphamide (CP). Lung fibrosis was assessed by measuring hydroxyproline and elastin levels and confirmed by histopathological examination. Oxidative stress was also observed in the CP group. Lung myeloperoxidase activity was significantly decreased in animals of the CP group. N‐acetyl‐β‐d‐glucosaminidase, leukotriene C4, and protein were increased in bronchoalveolar lavage fluid (BALF). Transforming growth factor‐β, interleukin ‐1β, and histamine were increased in both serum and BALF. All modulators markedly attenuated the altered biochemical parameters as compared to CP‐treated rats. These results suggest the possibility of using these treatments as protective agents with chemotherapy and as protective agents for lung fibrosis. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:461‐468, 2012; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.21447
Comparison of methods for quantifying dental wear caused by erosion and abrasion
Authors: Passos Vanara F. , Melo Mary A.S. , Vasconcellos Andréa Araújo , Rodrigues Lidiany K.A. , Santiago Sérgio L. 
Published: November 2012

AbstractVarious methods have been applied to evaluate the effect of erosion and abrasion. So, the aim of this study was to check the applicability of stylus profilometry (SP), surface hardness (SH) and focus‐variation 3D microscopy (FVM) to the analysis of human enamel and dentin subjected to erosion/abrasion. The samples were randomly allocated into four groups (n = 10): G1‐enamel/erosion, G2‐enamel/erosion plus abrasion, G3‐dentin/erosion, and G4‐dentin/erosion plus abrasion. The specimens were selected by their surface hardness, and they were subjected to cycles of demineralization (Coca‐Cola®‐60 s) and remineralization (artificial saliva‐60 min). For groups G2 and G4, the remineralization procedures were followed by toothbrushing (150 strokes). The above cycle was repeated 3×/day during 5 days. The samples were assessed using SH, SP, and FVM. For each substrate, the groups were compared using an unpaired t‐test, and Pearson correlation coefficients were calculated (α = 5%). For enamel, both profilometry technique showed greater surface loss when the erosion and abrasion processes were combined (P <0.05). The correlation analysis did not reveal any relationships among SH, SP, and FVM to G2 and G4. There were significant correlation coefficients (–0.70 and –0.67) for the comparisons between the FVM and SH methods in enamel and dentin, respectively, in G1 and G3. Choosing the ideal technique for the analysis of erosion depends on the type of dental substrate. SP was not sufficiently sensitive to measure the effects on dentin of erosion or erosion/abrasion. However, SP, FVM and SH were adequate for the detection of tissue loss and demineralization in enamel. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.
Intraoperative fluid management and complications following pancreatectomy
Authors: Grant Florence M. , Protic Mladjan , Gonen Mithat , Allen Peter , Brennan Murray F. 
Published: November 2012

AbstractBackgroundConsiderable debate exists as to appropriate perioperative fluid management. Data from several studies suggest that the amount of fluid administered perioperatively influences surgical outcome. Pancreatic resection is a major procedure in which complications are common. We examined 1,030 sequential patients who had undergone pancreatic resection at Memorial Sloan‐Kettering Cancer Center. We documented the prevalence and nature of their complications, and then correlated complications to intraoperative fluid administration.MethodsWe retrospectively examined 1,030 pancreatic resections performed at Memorial Sloan‐Kettering Cancer Center between May 2004 and December 2009 from our pancreatic database. Intraoperative administration of colloid and crystalloid was obtained from anesthesia records, and complication data from our institutional database.ResultsThe overall in‐hospital mortality was 1.7%. Operative mortality was due predominantly to intraabdominal infection. Sixty percent of the mortality resulted from intraabdominal complications related to the procedure. We did not demonstrate a clinically significant relationship between intraoperative fluid administration and complications, although minor statistical significance was suggested.ConclusionsIn this retrospective review of intraoperative fluid administration we were not able to demonstrate a clinically significant association between postoperative complications and intraoperative crystalloid and colloid fluid administration. A randomized controlled trial has been initiated to address this question. J. Surg. Oncol. 2013;107:529–535. © 2012 Wiley Periodicals, Inc.
Electron Beam Crosslinked Polyurethane Shape Memory Polymers with Tunable Mechanical Properties
Authors: Hearon Keith , Nash Landon D. , Volk Brent L. , Ware Taylor , Lewicki James P. , Voit Walter E. , Wilson Thomas S. , Maitland Duncan J. 
Published: November 2012

AbstractNovel electron beam crosslinked polyurethane shape memory polymers with advanced processing capabilities and tunable thermomechanical properties have been synthesized and characterized. We demonstrate the ability to manipulate crosslink density in order to finely tune rubbery modulus, strain capacity, ultimate tensile strength, recovery stress, and glass transition temperature. This objective is accomplished for the first time in a low‐molecular‐weight polymer system through the precise engineering of thermoplastic resin precursors suitable for mass thermoplastic processing. Neurovascular stent prototypes were fabricated by dip‐coating and laser machining to demonstrate processability.magnified image
Routes to Hydrogen Bonding Chain‐End Functionalized Polymers
Authors: Bertrand Arthur , Lortie Frédéric , Bernard Julien 
Published: November 2012

AbstractThe contribution of supramolecular chemistry to polymer science opens new perspectives for the design of polymer materials exhibiting valuable properties and easier processability due to the dynamic nature of non‐covalent interactions. Hydrogen bonding polymers can be used as supramolecular units for yielding larger assemblies that possess attractive features, arising from the combination of polymer properties and the responsiveness of hydrogen bonds. The post‐polymerization modification of reactive end‐groups is the most common procedure for generating such polymers. Examples of polymerizations mediated by hydrogen bonding‐functionalized precursors have also recently been reported. This contribution reviews the current synthetic routes toward hydrogen bonding sticker chain‐end functionalized polymers.
Individually Addressable Patterned Multilayer Microchambers for Site‐Specific Release‐On‐Demand
Authors: Kiryukhin Maxim V. , Gorelik Sergey R. , Man Shu Mei , Subramanian Gomathy Sandhya , Antipina Maria N. , Low Hong Yee , Sukhorukov Gleb B. 
Published: November 2012

AbstractPatterned arrays of light‐responsive microchambers are suggested as candidates for site‐specific release of chemicals in small and precisely defined quantities on demand. A composite film is made of poly(allylammonium)‐poly(styrene sulfonate) multilayers and gold nanoparticles incorporated between subsequent stacks of polyelectrolytes. The film shaped as microchambers is loaded with colloid particles or oil‐soluble molecules. The microchambers are sealed onto a glass slide precoated with an adhesive poly(diallyldimethylammonium)‐poly(styrene sulfonate) multilayer film. A focused laser beam is used for remote addressing the individual microchambers and site‐specific release of the loaded cargo.
Developing a short‐form structured diagnostic interview for common mental disorders using signal detection theory
Authors: Sunderland Matthew , Slade Tim , Andrews Gavin 
Published: November 2012

AbstractDiagnostic instruments must be relatively free from respondent burden and cost effective to administer whilst remaining faithful to the psychiatric nomenclature. It seems logical to develop short‐form alternatives to rather lengthy and complicated diagnostic interviews to facilitate large scale data collection. The current study examines one method, signal detection theory, for developing a short‐form interview based on the Composite International Diagnostic Interview version 3.0. The method was able to retain the smallest number of items to predict a lifetime and 30 day DSM‐IV diagnosis for 10 disorders. Concordance analyses between the full‐form and the short‐form modules, demonstrated an excellent level of agreement in the whole sample and various subsamples of the Australian population as well as in an international comparison sample of the US population. The good concordance between the long form and the short form demonstrates the ability of signal detection theory to assist in the development of valid short forms, which could replace lengthy diagnostic interviews when the aim is to reduce respondent burden and overall research costs. Copyright © 2012 John Wiley & Sons, Ltd.
Service design at diagnostic service centers
Authors: Wang Xiaofang , Debo Laurens G. , Scheller‐Wolf Alan , Smith Stephen F. 
Published: November 2012

AbstractWe study a service design problem in diagnostic service centers, call centers that provide medical advice to patients over the phone about what the appropriate course of action is, based on the caller's symptoms. Due to the tension between increased diagnostic accuracy and the increase in waiting times more in‐depth service requires, managers face a difficult decision in determining the optimal service depth to guide the diagnostic process. The specific problem we consider models the situation when the capacity (staffing level) at the center is fixed, and when the callers have both congestion‐ and noncongestion‐related costs relating to their call. We develop a queueing model incorporating these features and find that the optimal service depth can take one of two different structures, depending on factors such as the nurses' skill level and the maximum potential demand. Sensitivity analyses of the two optimal structures show that they are quite different. In some situations, it may (or may not) be optimal for the manager to try to expand the demand at the center, and increasing skill level may (or may not) increase congestion. © 2012 Wiley Periodicals, Inc. Naval Research Logistics, 2012
Facile Oxidative Rearrangements Using Hypervalent Iodine Reagents
Authors: Singh Fateh V. , Rehbein Julia , Wirth Thomas 
Published: November 2012

AbstractAromatic substituents migrate in a novel oxidative cyclization mediated by iodine(III) reagents. 4‐Arylbut‐3‐enoic acids are cyclized and rearranged to 4‐arylfuran‐2(5H)‐ones by hypervalent iodine compounds in good to excellent yields under mild reaction conditions. Other ring sizes are also accessible. The mechanism of the reaction is described in detail, and calculations highlight the cationic nature of the intermediates in the rearrangement. The fast access to heavily substituted furanones is used for the synthesis of biologically active derivatives.