bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exposure-associated DNA methylation among people exposed to multiple industrial pollutants
Authors: Chen Chi-Hsin Sally , Yuan Tzu-Hsuen , Lu Tzu-Pin , Lee Hsin-Ying , Chen Yi-Hsuen , Lai Liang-Chuan , Tsai Mong-Hsun , Chuang Eric Y. , Chan Chang-Chuan 
Published: August 2024

Abstract
Background
Current research on the epigenetic repercussions of exposure to a combination of pollutants is limited. This study aims to discern DNA methylation probes associated with exposure to multiple pollutants, serving as early effect markers, and single-nucleotide polymorphisms (SNPs) as surrogate indicators for population susceptibility. The investigation involved the analysis of urine exposure biomarkers for 11 heavy metals (vanadium, arsenic, mercury, cadmium, chromium, nickel, lead, manganese, copper, strontium, thallium), polycyclic aromatic hydrocarbon (PAHs) (1-hydroxypyrene), genome-wide DNA methylation sequencing, and SNPs array on all study participants. The data were integrated with metabolomics information and analyzed both at a community level based on proximity to home addresses relative to the complex and at an individual level based on exposure biomarker concentrations.

Results
On a community level, 67 exposure-related CpG probes were identified, while 70 CpG probes were associated with urine arsenic concentration, 2 with mercury, and 46 with vanadium on an individual level. These probes were annotated to genes implicated in cancers and chronic kidney disease. Weighted quantile sum regression analysis revealed that vanadium, mercury, and 1-hydroxypyrene contributed the most to cg08238319 hypomethylation. cg08238319 is annotated to the aryl hydrocarbon receptor repressor (AHRR) gene, and AHRR hypomethylation was correlated with an elevated risk of lung cancer. AHRR was further linked to deregulations in phenylalanine metabolism, alanine, aspartate, and glutamate metabolism, along with heightened oxidative stress. Additionally, three SNPs (rs11085020, rs199442, and rs10947050) corresponding to exposure-related CpG probes exhibited significant interaction effects with multiple heavy metals and PAHs exposure, and have been implicated in cancer progression and respiratory diseases.

Conclusion
Our findings underscore the pivotal role of AHRR methylation in gene-environment interactions and highlight SNPs that could potentially serve as indicators of population susceptibility in regions exposed to multiple heavy metals and PAHs.
Early reapplication of prone position during venovenous ECMO for acute respiratory distress syndrome: a prospective observational study and propensity-matched analysis
Authors: Wang Rui , Tang Xiao , Li Xuyan , Li Ying , Liu Yalan , Li Ting , Zhao Yu , Wang Li , Li Haichao , Li Meng , Li Hu , Tong Zhaohui , Sun Bing 
Published: August 2024

Abstract
Background
A combination of prone positioning (PP) and venovenous extracorporeal membrane oxygenation (VV-ECMO) is safe, feasible, and associated with potentially improved survival for severe acute respiratory distress syndrome (ARDS). However, whether ARDS patients, especially non-COVID-19 patients, placed in PP before VV-ECMO should continue PP after a VV-ECMO connection is unknown. This study aimed to test the hypothesis that early use of PP during VV-ECMO could increase the proportion of patients successfully weaned from ECMO support in severe ARDS patients who received PP before ECMO.

Methods
In this prospective observational study, patients with severe ARDS who were treated with VV-ECMO were divided into two groups: the prone group and the supine group, based on whether early PP was combined with VV-ECMO. The proportion of patients successfully weaned from VV-ECMO and 60-day mortality were analyzed before and after propensity score matching.

Results
A total of 165 patients were enrolled, 50 in the prone and 115 in the supine group. Thirty-two (64%) and 61 (53%) patients were successfully weaned from ECMO in the prone and the supine groups, respectively. The proportion of patients successfully weaned from VV-ECMO in the prone group tended to be higher, albeit not statistically significant. During PP, there was a significant increase in partial pressure of arterial oxygen (PaO2) without a change in ventilator or ECMO settings. Tidal impedance shifted significantly to the dorsal region, and lung ultrasound scores significantly decreased in the anterior and posterior regions. Forty-five propensity score-matched patients were included in each group. In this matched sample, the prone group had a higher proportion of patients successfully weaned from VV-ECMO (64.4% vs. 42.2%; P = 0.035) and lower 60-day mortality (37.8% vs. 60.0%; P = 0.035).

Conclusions
Patients with severe ARDS placed in PP before VV-ECMO should continue PP after VV-ECMO support. This approach could increase the probability of successful weaning from VV-ECMO.

Trial Registration
ClinicalTrials.Gov: NCT04139733. Registered 23 October 2019.
Recovery of plant nutrients from human excreta and domestic wastewater for reuse in agriculture: a systematic map and evidence platform
Authors: Macura Biljana , Metson Geneviève S. , McConville Jennifer R. , Harder Robin 
Published: August 2024

Abstract
Background
Achieving a more circular and efficient use of nutrients found in human excreta and domestic (municipal) wastewater is an integral part of mitigating aquatic nutrient pollution and nutrient insecurity. A synthesis of research trends readily available to various stakeholders is much needed. This systematic map collates and summarizes scientific research on technologies that facilitate the recovery and reuse of plant nutrients and organic matter found in human excreta and domestic wastewater. We present evidence in a way that can be navigated easily. We hope this work will help with the uptake and upscaling of new and innovative circular solutions for the recovery and reuse of nutrients.

Methods
The systematic map consists of an extension of two previous related syntheses. Searches were performed in Scopus and Web of Science in English. Records were screened on title and abstract, including consistency checking. Coding and meta-data extraction included bibliographic information, as well as recovery pathways. The evidence from the systematic map is embedded in an online evidence platform that, in an interactive manner, allows stakeholders to visualize and explore the systematic map findings, including knowledge gaps and clusters.

Results
The evidence base includes a total of 10 950 articles describing 11 489 recovery pathways. Most of the evidence base is about recovery technologies (41.9%) and the reuse of recovered products in agriculture (53.4%). A small proportion of the evidence base focuses on the characteristics of recovered products (4.0%) and user acceptance and perceptions of nutrient recovery and reuse (0.7%).

Conclusions
Most studies we mapped focused on nutrient recovery from ‘conventional’ systems, that is, from centralized sewer and wastewater treatment systems that produce biosolids and a treated effluent. While we also found substantial research on nutrient recovery from source-separated urine, and to some extent also on nutrient recovery from source-separated excreta (notably blackwater), the body of research on nutrient recovery from source-separated feces was relatively small. Another knowledge gap is the relative lack of research on the recovery of potassium. More research is also needed on user acceptance of different recovery technologies and recovered products.
CO-driven electron and carbon flux fuels synergistic microbial reductive dechlorination
Authors: Wang Jingjing , Li Xiuying , Jin Huijuan , Yang Shujing , Yu Lian , Wang Hongyan , Huang Siqi , Liao Hengyi , Wang Xuhao , Yan Jun , Yang Yi 
Published: August 2024

Abstract
Background
Carbon monoxide (CO), hypothetically linked to prebiotic biosynthesis and possibly the origin of the life, emerges as a substantive growth substrate for numerous microorganisms. In anoxic environments, the coupling of CO oxidation with hydrogen (H2) production is an essential source of electrons, which can subsequently be utilized by hydrogenotrophic bacteria (e.g., organohalide-respring bacteria). While Dehalococcoides strains assume pivotal roles in the natural turnover of halogenated organics and the bioremediation of chlorinated ethenes, relying on external H2 as their electron donor and acetate as their carbon source, the synergistic dynamics within the anaerobic microbiome have received comparatively less scrutiny. This study delves into the intriguing prospect of CO serving as both the exclusive carbon source and electron donor, thereby supporting the reductive dechlorination of trichloroethene (TCE).

Results
The metabolic pathway involved anaerobic CO oxidation, specifically the Wood-Ljungdahl pathway, which produced H2 and acetate as primary metabolic products. In an intricate microbial interplay, these H2 and acetate were subsequently utilized by Dehalococcoides, facilitating the dechlorination of TCE. Notably, Acetobacterium emerged as one of the pivotal collaborators for Dehalococcoides, furnishing not only a crucial carbon source essential for its growth and proliferation but also providing a defense against CO inhibition.

Conclusions
This research expands our understanding of CO’s versatility as a microbial energy and carbon source and unveils the intricate syntrophic dynamics underlying reductive dechlorination.

Graphical Abstract
Migratory-derived resources induce elongated food chains through middle-up food web effects
Authors: Moccetti Coralie , Sperlich Nicola , Saboret Grégoire , ten Brink Hanna , Brodersen Jakob 
Published: August 2024

Abstract
Background
Seasonal movements of animals often result in the transfer of large amounts of energy and nutrients across ecosystem boundaries, which may have large consequences on local food webs through various pathways. While this is known for both terrestrial- and aquatic organisms, quantitative estimates on its effects on food web structure and identification of key pathways are scarce, due to the difficulty in obtaining replication on ecosystem level with negative control, i.e. comparable systems without migration.

Methods
In this study, we estimate the impact of Arctic charr (Salvelinus alpinus) migration on riverine ecosystem structure, by comparing multiple streams with strictly resident populations above natural migration barriers with streams below those barriers harboring partially migratory populations. We compared density estimates and size structure between above and below populations. Diet differences were examined through the analysis of stomach contents, changes in trophic position were examined by using stable isotopes. To infer growth rate of resident individuals, back-growth calculation was performed using otoliths.

Results
We find higher densities of small juveniles in partially migratory populations, where juvenile Arctic charr show initially lower growth, likely due to higher intraspecific competition. After reaching a size, where they can start feeding on eggs and smaller juveniles, which are both more frequent in partially migratory populations, growth surpasses that of resident populations. Cannibalism induced by high juvenile densities occurred almost exclusively in populations with migration and represents an altered energy pathway to the food web. The presence of large cannibalistic charr feeding on smaller ones that have a similar trophic level as charr from strictly resident populations (based on stomach content) coupled with steeper δ15N-size regression slopes illustrate the general increase of food chain length in systems with migration.

Conclusions
Our results thus suggest that the consumption of migration-derived resources may result in longer food chains through middle-up rather than bottom-up effects. Furthermore, by occupying the apex of the food chain and feeding on juvenile conspecifics, resident individuals experience reduced competition with their young counterparts, which potentially balances their fitness with migratory individuals.
Morphological MRI features as prognostic indicators in brain metastases
Authors: Ocaña-Tienda Beatriz , Pérez-Beteta Julián , Ortiz de Mendivil Ana , Asenjo Beatriz , Albillo David , Pérez-Romasanta Luís A. , LLorente Manuel , Carballo Natalia , Arana Estanislao , Pérez-García Víctor M. 
Published: August 2024

Abstract
Background
Stereotactic radiotherapy is the preferred treatment for managing patients with fewer than five brain metastases (BMs). However, some lesions recur after irradiation. The purpose of this study was to identify patients who are at a higher risk of failure, which can help in adjusting treatments and preventing recurrence.

Methods
In this retrospective multicenter study, we analyzed the predictive significance of a set of interpretable morphological features derived from contrast-enhanced (CE) T1-weighted MR images as imaging biomarkers using Kaplan–Meier analysis. The feature sets studied included the total and necrotic volumes, the surface regularity and the CE rim width. Additionally, we evaluated other nonmorphological variables and performed multivariate Cox analysis.

Results
A total of 183 lesions in 128 patients were included (median age 61 [31–95], 64 men and 64 women) treated with stereotactic radiotherapy (57% single fraction, 43% fractionated radiotherapy). None of the studied variables measured at diagnosis were found to have prognostic value. However, the total and necrotic volumes and the CE rim width measured at the first follow-up after treatment and the change in volume due to irradiation can be used as imaging biomarkers for recurrence. The optimal classification was achieved by combining the changes in tumor volume before and after treatment with the presence or absence of necrosis (p <  < 0.001).

Conclusion
This study demonstrated the prognostic significance of interpretable morphological features extracted from routine clinical MR images following irradiation in brain metastases, offering valuable insights for personalized treatment strategies.
Cortical dynamics of perception as trains of coherent gamma oscillations, with the pulvinar as central coordinator
Authors: Farineau J. , Lestienne R. 
Published: August 2024

AbstractSynchronization of spikes carried by the visual streams is strategic for the proper binding of cortical assemblies, hence for the perception of visual objects as coherent units. Perception of a complex visual scene involves multiple trains of gamma oscillations, coexisting at each stage in visual and associative cortex. Here, we analyze how this synchrony is managed, so that the perception of each visual object can emerge despite this complex interweaving of cortical activations. After a brief review of structural and temporal facts, we analyze the interactions which make the oscillations coherent for the visual elements related to the same object. We continue with the propagation of these gamma oscillations within the sensory chain. The dominant role of the pulvinar and associated reticular thalamic nucleus as cortical coordinator is the common thread running through this step-by-step description. Synchronization mechanisms are analyzed in the context of visual perception, although the present considerations are not limited to this sense. A simple experiment is described, with the aim of assessing the validity of the elements developed here. A first set of results is provided, together with a proposed method to go further in this investigation.
Pricing multi-asset options with tempered stable distributions
Authors: Xia Yunfei , Grabchak Michael 
Published: August 2024

AbstractWe derive methods for risk-neutral pricing of multi-asset options, when log-returns jointly follow a multivariate tempered stable distribution. These lead to processes that are more realistic than the better known Brownian motion and stable processes. Further, we introduce the diagonal tempered stable model, which is parsimonious but allows for rich dependence between assets. Here, the number of parameters only grows linearly as the dimension increases, which makes it tractable in higher dimensions and avoids the so-called “curse of dimensionality.” As an illustration, we apply the model to price multi-asset options in two, three, and four dimensions. Detailed goodness-of-fit methods show that our model fits the data very well.
Delayed recovery of consciousness from anesthesia due to exacerbation of hydrocephalus caused by a ventriculoperitoneal shunt malfunction during general anesthesia in the prone position: a case report
Authors: Miyamoto Yosuke , Kawasaki Takashi , Nakamura Shingo , Hirata Naoyuki 
Published: August 2024

Abstract
Background
Dysfunction of ventriculoperitoneal (VP) shunts can lead to decreased levels of consciousness. We report a case of delayed emergence from anesthesia due to the malfunction of a VP shunt during neurosurgery in the prone position.

Case presentation
A 75-year-old male with a history of VP shunt for a fourth ventricle obstruction underwent cerebral vascular anastomosis in the prone position. His preoperative level of consciousness was clear. The surgery under general anesthesia was completed without any particular issues. After discontinuation of anesthesia, the patient did not awaken for over an hour. Postoperative CT revealed exacerbated hydrocephalus, likely from VP shunt occlusion. After pumping the reservoir of the VP shunt, the patient regained consciousness. He was extubated and discharged from ICU on the second postoperative day with no neurological issues.

Conclusion
For surgical patients with a VP shunt, anesthesia management must consider the risk of shunt malfunction due to patient positioning.
Psychological assessment in school contexts: ethical issues and practical guidelines
Authors: Cadime Irene , Mendes Sofia A. 
Published: August 2024

Abstract
Background
Psychological assessment in school settings involves a range of complexities and ethical dilemmas that practitioners must navigate carefully. This paper provides a comprehensive review of common issues faced by school psychologists during assessments, discussing best practices and ethical guidelines based on codes from various professional organizations.

Methods
We examine the entire assessment process, from pre-assessment considerations like informed consent and instrument selection to post-assessment practices involving results communication and confidentiality. Key ethical concerns addressed include fairness in assessment, cultural and linguistic appropriateness of testing materials, and issues surrounding informed consent.

Results
Specific challenges discussed include selecting appropriate assessment instruments that reflect the diverse needs and backgrounds of students, ensuring fairness and removing bias in testing, and effectively communicating results to various stakeholders while maintaining confidentiality. We emphasize the importance of multi-source, multi-method assessment approaches and the critical role of ongoing professional development in ethical practice.

Conclusion
By adhering to established ethical standards and best practices, school psychologists can effectively support the educational and developmental needs of students. This paper outlines actionable recommendations and ethical considerations to help practitioners enhance the accuracy, fairness, and impact of their assessments in educational settings.
Pathogenicity of native strains of Bacillus thuringiensis, Beauveria bassiana and Metarhizium rileyi as entomopathogens against the polyphagous borer, Conogethes punctiferalis (Guenée) (Crambidae: Lepidoptera)
Authors: Duraimurugan P. , Bharathi E. , Dharavath Neethu Roy , Selvam Hariharan 
Published: August 2024

Abstract
Background
The shoot and fruit borer, Conogethes punctiferalis (Guenée) (Crambidae: Lepidoptera), is a significant pest causing substantial economic losses across various crops. The need for alternative control methods has prompted the exploration of biological control using entomopathogenic fungi and bacteria. In this study, the pathogenicity of Beauveria bassiana, Metarhizium (Nomuraea) rileyi and Bacillus thuringiensis (Bt) against C. punctiferalis larvae and pupae was assessed through laboratory bioassays.

Results
Various concentrations of B. bassiana and M. rileyi spores, i.e. 1 × 102, 1 × 104, 1 × 106, 1 × 107 and 1 × 108 spores/ml, were tested alongside controls. Additionally, five strains of Bt (IIOR Bt-145, Bt-154, Bt-171, Bt-172 and Bt-127) were evaluated at concentrations of 0.5, 1.0, 1.5, 2.0 and 2.5 mg/ml. The LC50 values for B. bassiana and M. rileyi were 7.9 × 105 spores/ml and 8.7 × 104 spores/ml, respectively, after 4 and 6 days of post-treatment using the spray method. In the larval dip method, the LC50 values were 4.8 × 103 spores/ml for B. bassiana and 2.0 × 104 spores/ml for M. rileyi after 5 and 6 days of treatment, respectively. For pupae, the LC50 values were 1.2 × 106 spores/ml for B. bassiana and 4.3 × 104 spores/ml for M. rileyi after 4 and 7 days of treatment, respectively. Similarly, the five strains of Bt were effective against C. punctiferalis. However, Bt-154 demonstrated the highest efficacy, with LC50 values of 0.66 mg/ml in the spray method and 0.79 mg/ml in the larval dip method after 5 days of post-treatment.

Conclusion
The potential of entomopathogenic isolates as biocontrol agents against C. punctiferalis provided a promising alternative to synthetic insecticides in pest management. The efficacy of B. bassiana, M. rileyi and Bt strains suggests their suitability for integrated pest management strategies, potentially reducing reliance on chemical pesticides and minimizing the environmental impacts. Further field studies are warranted to validate the efficacy and practicality of these biocontrol agents in real-world agricultural settings.
Disorders of organic acid metabolism and epilepsy
Authors: Shi Yuqing , Wei Zihan , Feng Yan , Gan Yajing , Li Guoyan , Deng Yanchun 
Published: August 2024

AbstractEpilepsy can be caused by a variety of causes, such as inborn errors of metabolism, organic acid disorders are the most significant type of metabolic disorders that cause seizures. The clinical manifestations of these diseases are generally nonspecific, and the types of seizures are different. Screening for multisystem clinical symptoms and identifying the underlying etiology are crucial for early treatment of epileptic seizures. This article provides a comprehensive summary of the pathogenesis, clinical features, diagnosis and treatment of epilepsy associated with organic acid metabolism disorders. Furthermore, relevant literature has also been reviewed to assist clinicians in the diagnosis of cases characterized by the coexistence of multisystemic symptoms and epileptic manifestations.
Stability-indicating liquid chromatographic method development and validation for quantification of trifarotene in pure and topical drug product
Authors: Usman Muhammad , Shafique Muhammad Bilal 
Published: August 2024

Abstract
Background
Trifarotene is effective for treating acne and other skin issues. To ensure its quality and meet regulatory standards, a reverse phase liquid chromatography (RP-LC) stability-indicating method was developed and validated for its quantification in pure and topical dosage forms. An isocratic elution chromatographic method was employed, using an octadecylsilyl silica gel-packed column (150 mm × 4.6 mm, 3 µm particle size). The mobile phase was a mixture of phosphate buffer and acetonitrile (40:60 v/v). Chromatographic conditions included a flow rate of 0.5 mL/min, column temperature of 40 °C, detection at 265 nm, and injection volume of 20 µL.

Results
The developed analytical method reports the retention time of trifarotene 11.2 min, higher theoretical plate count, asymmetric peak, and good resolution between the peaks of trifarotene, phenoxyethanol, and environmentally generated impurities.

Conclusion
The analytical method has been found to be linear, accurate, robust, specific, and selective for impurities produced during forced degradation studies. The proposed analytical method can be utilized for routine pharmaceutical analysis of trifarotene to judge its quality and safety.