bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contrast Sensitivity Better Reflects Wide-Field Swept-Source Optical Coherence Tomography Angiography Vascular Metrics Among Healthy Eyes Compared to Visual Acuity
Authors: Baldwin Grace , Tracy Jack , Vingopoulos Filippos , Munsell Mary , Bennett Cade , Rodriguez Jocelyn Marie , Choi Hanna , Garg Itika , Tandias Rachel , Wescott Hannah , Finn Matthew J. , Razavi Peyman , Bannerman Augustine , Zeng Rebecca , Vavvas Demetrios G. , Husain Deeba , Kim Leo A. , Patel Nimesh A. , Miller John B. 
Published: June 2024

Background and Objective:
To evaluate the association between widefield swept-source optical coherence tomography angiography (WF SS-OCTA) and visual function in healthy eyes.

Patients and Methods:
Fifty-seven eyes of 45 patients were evaluated with visual acuity (VA), contrast sensitivity (CS), and WF SS-OCTA (3 × 3, 6 × 6, and 12 × 12 mm images) on the same day. Mixed-effects multivariable regression analyses were performed.

Results:

Contrast sensitivity metrics, including CS between 6 to 18 cycles per degree (cpd) and area under the logarithm CS function, were significantly associated with vessel density (VD) and vessel skeletonized density (VSD), whereas VA was not. The largest effect size was between CS at 18 cpd and VD (β = 0.41,
P
= 0.007) and VSD (β = 0.42,
P
= 0.006) on 12 × 12 mm images.

Conclusions:
Reduced VSD and VD on WF SSOCTA was significantly associated with decreased CS, whereas VA was not. These results suggest CS could serve as a screening tool for early stage retinal and neurologic disorders.

[
Ophthalmic Surg Lasers Imaging Retina
2024;55:494–502.]
La fin d’une expérience éducative alternative. Gouvernance locale de l’éducation et usure militante
Authors: Allam Marie-Charlotte 
Published: June 2024

Cet article étudie les ressorts du désengagement d’enseignants qui se sont impliqués, sur plusieurs décennies, dans une expérience éducative autogestionnaire à la Villeneuve de Grenoble. Il observe les transformations de leurs mobilisations et de leurs pratiques militantes en lien avec le durcissement du contrôle bureaucratique et l’introduction d’instruments de gestion par projet entre les années 1980 et 2000. Sur le temps court, cette transformation de l’encadrement administratif suscite des mobilisations défensives, allant de l’opposition frontale à l’adaptation stratégique. Sur le temps long, elle alimente une usure militante, qui conduit à la disparition progressive de l’expérimentation.
Enseigner la gestion stratégique de l’exploitation : la méthode 3S en question
Authors: Gafsi Mohamed , Brochard Jean-Marie , Cubizole Magali , Legay Emmanuel , Melac Claire , Zegdane-Loisel Coralie 
Published: June 2024

L’enseignement agricole est à l’épreuve, ces dernières années, des enjeux de la transition agroécologique. Dans ce contexte de changement et d’incertitude, la gestion stratégique de l’exploitation agricole, et son enseignement, sont devenus une nécessité. Mais force est de constater que les enseignants font face à de multiples difficultés et manquent de ressources pour enseigner de manière cohérente la gestion stratégique et l’analyse de durabilité de l’exploitation agricole en conservant les acquis de l’approche systémique. Les auteurs proposent une démarche méthodologique qui vise à surmonter ces difficultés et à outiller les enseignants. Structurée en trois temps, la méthode 3S aide à analyser dans le temps long des stratégies suivies, puis à saisir la logique de fonctionnement et à évaluer la performance globale de l’exploitation, pour élaborer ensuite des axes stratégiques pour les années à venir. Cette méthode a été construite par des enseignants de lycées agricoles, dans le cadre d’un travail de recherche-action au sein du GAP – Groupe d’animation et de professionnalisation – Économie-Gestion. La méthode s’adresse à la communauté des enseignants et tout formateur ou conseiller amené à accompagner des agriculteurs dans des stratégies pour des systèmes agricoles durables.
Faire avec les normes de Parcoursup : le cas des réorienté·es
Authors: Valarcher Marion , Bugeja-Bloch Fanny , Couto Marie-Paule , Frouillou Leïla 
Published: June 2024

Une des nouveautés de Parcoursup est d’intégrer de plus en plus les réorientations vers une première année d’enseignement supérieur français. À partir d’une étude qualitative des dossiers de réorientation en sociologie, cet article interroge la manière dont le fonctionnement du dispositif Parcoursup impose des normes institutionnelles désajustées aux parcours non linéaires des étudiantes en réorientation. Les désajustements sont doubles : d’une part, les catégories de la plateforme sont en décalage avec les temporalités des parcours étudiant·es, et d’autre part, l’ordre de remplissage des dossiers ne correspond pas nécessairement à la lecture qui en est faite par les commissions. Cela explique en partie que les classements soient souvent défavorables aux réorienté·es par rapport aux néo-bachelier·es.
En quête de projet
Authors: Guichard Ambre 
Published: June 2024

La demande de formalisation d’un « projet de formation motivé » sur Parcoursup introduit une épreuve de justification de la cohérence du parcours dès lors partie prenante de la sélection-orientation à l’entrée des formations universitaires. À partir d’une étude de cas portant sur les étudiant·es qui se sont réorienté·es malgré l’adéquation de leur choix d’orientation, l’article montre que le projet ne permet pas nécessairement de répondre à l’exigence de cohérence en cours d’études, et qu’il nécessite même parfois un travail de justification supplémentaire. L’analyse des argumentaires mobilisés pour justifier le passage d’un projet à un autre met en évidence la manière dont les étudiant·es se sont progressivement acculturé·es à une conception temporalisée de la réussite. En s’appropriant le projet en tant qu’instrument de réflexivité, les étudiant·es travaillent finalement à accepter le fait d’avoir renoncé à leurs aspirations initiales.
Mesenchymal stem cells-extracellular vesicles alleviate pulmonary fibrosis by regulating immunomodulators
Authors: Gao Ying , Liu Mei-Fang , Li Yang , Liu Xi , Cao Yu-Jie , Long Qian-Fa , Yu Jun , Li Jian-Ying 
Published: June 2024

BACKGROUND
Pulmonary fibrosis (PF) is a chronic interstitial lung disease characterized by fibroblast proliferation and extracellular matrix formation, causing structural damage and lung failure. Stem cell therapy and mesenchymal stem cells-extracellular vesicles (MSC-EVs) offer new hope for PF treatment.
AIM
To investigate the therapeutic potential of MSC-EVs in alleviating fibrosis, oxidative stress, and immune inflammation in A549 cells and bleomycin (BLM)-induced mouse model.
METHODS
The effect of MSC-EVs on A549 cells was assessed by fibrosis markers [collagen I and α-smooth muscle actin (α-SMA), oxidative stress regulators [nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), and inflammatory regulators [nuclear factor-kappaB (NF-κB) p65, interleukin (IL)-1β, and IL-2]. Similarly, they were assessed in the lungs of mice where PF was induced by BLM after MSC-EV transfection. MSC-EVs ion PF mice were detected by pathological staining and western blot. Single-cell RNA sequencing was performed to investigate the effects of the MSC-EVs on gene expression profiles of macrophages after modeling in mice.
RESULTS
Transforming growth factor (TGF)-β1 enhanced fibrosis in A549 cells, significantly increasing collagen I and α-SMA levels. Notably, treatment with MSC-EVs demonstrated a remarkable alleviation of these effects. Similarly, the expression of oxidative stress regulators, such as Nrf2 and HO-1, along with inflammatory regulators, including NF-κB p65 and IL-1β, were mitigated by MSC-EV treatment. Furthermore, in a parallel manner, MSC-EVs exhibited a downregulatory impact on collagen deposition, oxidative stress injuries, and inflammatory-related cytokines in the lungs of mice with PF. Additionally, the mRNA sequencing results suggested that BLM may induce PF in mice by upregulating pulmonary collagen fiber deposition and triggering an immune inflammatory response. The findings collectively highlight the potential therapeutic efficacy of MSC-EVs in ameliorating fibrotic processes, oxidative stress, and inflammatory responses associated with PF.
CONCLUSION
MSC-EVs could ameliorate fibrosis in vitro and in vivo by downregulating collagen deposition, oxidative stress, and immune-inflammatory responses.
Umbilical cord mesenchymal stem cell exosomes alleviate necrotizing enterocolitis in neonatal mice by regulating intestinal epithelial cells autophagy
Authors: Zhu Lin , He Lu , Duan Wu , Yang Bo , Li Ning 
Published: June 2024

BACKGROUND
Necrotizing enterocolitis (NEC) is a severe gastrointestinal disease that affects premature infants. Although mounting evidence supports the therapeutic effect of exosomes on NEC, the underlying mechanisms remain unclear.
AIM
To investigate the mechanisms underlying the regulation of inflammatory response and intestinal barrier function by umbilical cord mesenchymal stem cell (UCMSCs) exosomes, as well as their potential in alleviating NEC in neonatal mice.
METHODS
NEC was induced in 5-d-old C57BL/6 pups through hypoxia and gavage feeding of formula containing lipopolysaccharide (LPS), after which the mice received human UCMSC exosomes (hUCMSC-exos). The control mice were allowed to breastfeed with their dams. Ileal tissues were collected from the mice and analyzed by histopathology and immunoblotting. Colon tissues were collected from NEC neonates and analyzed by immunofluorescence. Molecular biology and cell culture approaches were employed to study the related mechanisms in intestinal epithelial cells.
RESULTS
We found that autophagy is overactivated in intestinal epithelial cells during NEC, resulting in reduced expression of tight junction proteins and an increased inflammatory response. The ability of hUCMSC-exos to ameliorate NEC in a mouse model was dependent on decreased intestinal autophagy. We also showed that hUCMSC-exos alleviate the inflammatory response and increase migration ability in intestinal epithelial cells induced by LPS.
CONCLUSION
These results contribute to a better understanding of the protective mechanisms of hUCMSC-exos against NEC and provide a new theoretical and experimental foundation for NEC treatment. These findings also enhance our understanding of the role of the autophagy mechanism in NEC, offering potential avenues for identifying new therapeutic targets.
Using Dried Blood Spots to Quantitatively Detect Anti–SARS-CoV-2 IgG Antibodies by ELISA: A Validation Study
Authors: Ylade Michelle , Aziz Asma Binte , Daag Jedas Veronica , Crisostomo Maria Vinna , Agrupis Kristal-An , Maronilla Maria Angela , Sye Lim Hong Chloe , Kim Hwa Young , Njau Irene , Lopez March Helena Jane , Deen Jacqueline , Kim Deok Ryun , You Young Ae , Kang Sophie S. Y. , Marks Florian , Tadesse Birkneh Tilahun 
Published: June 2024

ABSTRACT.
SARS-CoV-2 serological testing is useful to determine seroprevalence, epidemiological trends, and the extent of transmission. The collection and transport of serum samples can be logistically challenging, especially in remote underserved areas. Dried blood spots (DBSs) would allow easier sample collection and logistical handling compared with standard serum collection, particularly for extensive and repeated SARS-CoV-2 serosurveys. We evaluated the sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of the IgG ELISA (Wantai, Beijing, China) using DBSs against sera for the quantitative detection of SARS-CoV-2 IgG antibody. The IgG ELISA was used to test paired sera and DBSs obtained from individuals with recent virologically confirmed COVID-19 illness and banked paired sera and DBSs collected before the COVID-19 pandemic. We found that 100/100 (100%) seropositive samples were positive using DBSs, and 193/194 (99%) seronegative samples were negative using DBSs. Compared with sera, the DBS method had a 100% sensitivity, 99% specificity, 99% PPV, and 100% NPV. Use of DBSs for SARS-CoV-2 household or population serosurveys may be considered in situations with limitations in sample collection, shipment, and storage.
Gamma-Irradiated Female Aedes aegypti Mosquitoes Exhibit Greater Susceptibility to Mayaro Virus
Authors: Trefry Stephanie V. , Aldridge Robert L. , Sprague Thomas R. , Lowen Robert G. , Erasmus Jesse H. , Pitt Margaret L. , Hahn Daniel A. , Nasar Farooq , Gibson Seth , Linthicum Kenneth J. 
Published: June 2024

ABSTRACT.
Mayaro virus (MAYV) is an alphavirus endemic in many parts of Central and South America transmitted to humans by Aedes aegypti. Currently, there is no vaccine or treatment of Mayaro infection, and therefore it is essential to control transmission by reducing populations of Ae. aegypti. Unfortunately, Ae. aegypti are extremely difficult to control with traditional integrated vector management (IVM) because of factors such as growing resistance to a dwindling list of registered insecticides and cryptic immature and adult habitats. The sterile insect technique (SIT) by irradiation is gaining traction as a novel supplemental tool to IVM. The SIT is being used operationally to release large numbers of sterilized colony-reared male mosquitoes in an intervention area to overwhelm females in the natural population, eventually causing population decline because of high frequencies of unfertilized eggs. However, little is known about the effect of irradiation on vector competence for mosquito-borne viruses such as MAYV in females that may be accidentally reared, irradiated, and released alongside males. In this investigation, we exposed female Ae. aegypti pupae to radiation and evaluated vector competence after inoculation with MAYV. Infection and dissemination rates of irradiated (10 and 40 Gy) Ae. aegypti were higher than those of non-irradiated cohorts at 7 and 14 days after infection. Although these results indicate a need to maintain effective sex sorting prior to irradiation and release of Ae. aegypti, our results are consistent with several previous observations that vectorial capacity and vector competence are likely lower in irradiated than in nonirradiated females.
Results of Integrated Transmission Assessment Surveys for Lymphatic Filariasis and Malaria in Haiti, 2017–2022
Authors: Désir Luccène , Hamre Karen E. S. , Beau De Rochars Valéry Madsen , Lemoine Jean F. , Telfort Marc-Aurèle , Noland Gregory S. 
Published: June 2024

ABSTRACT.
Haiti is endemic for lymphatic filariasis (LF) and malaria, two mosquito-transmitted parasitic diseases targeted for elimination. The World Health Organization recommends a transmission assessment survey (TAS-1) to determine if LF prevalence is significantly beneath putative transmission thresholds (<2% antigen prevalence in Haiti, where Culex is the primary vector for Wuchereria bancrofti) to stop mass drug administration (MDA). Repeated TASs (TAS-2 and TAS-3) are recommended at 2–3-year intervals during post-treatment surveillance. From 2017 to 2022, The Carter Center assisted the Haitian Ministry of Public Health and Population in conducting 15 TASs in 11 evaluation units (EUs) encompassing 54 of the country’s 146 districts. Children 6–7 years old were assessed for circulating filarial antigen (CFA) by Filariasis Test Strip: n = 5,239 in TAS-1; n = 11,866 in TAS-2; and n = 1,842 in TAS-3, of whom eight (0.15%), 20 (0.17%), and eight (0.43%) tested positive, respectively. The number of positive results in children was less than the threshold in each EU. When available, participants (n = 16,663) were also tested for malaria by rapid diagnostic test, with 31 (0.19%) children testing positive for Plasmodium falciparum. Integrated TASs provided an efficient means to collect epidemiological data for LF and malaria in Haiti. Results indicated thresholds for stopping and maintaining the halt of MDA for LF have been achieved in all EUs, with the halt of MDA for 571,358 people in four districts and the first TAS-3 surveys conducted in Haiti. Investigations are needed to assess the potential of ongoing LF transmission, especially in areas where CFA-positive samples were detected in TAS-3.
Trends in the Prevalence of Antimicrobial Resistance in Escherichia coli Isolated from Outpatient Urine Cultures in French Amazonia
Authors: Nkontcho Djamkeba Flaubert , Sainte-Rose Vincent , Lontsi Ngoulla Guy Roger , Roujansky Ariane , Abboud Philippe , Walter Gaelle , Houcke Stephanie , Demar Magalie , Kallel Hatem , Pujo Jean Marc , Djossou Felix 
Published: June 2024

ABSTRACT.
Antimicrobial resistance (AMR) in the community is increasing worldwide. We aimed to assess AMR trends in Escherichia coli from the community urine isolates in French Amazonia. We conducted a retrospective study from January 2016 to December 2022 in the Cayenne General Hospital microbiology laboratory (French Guiana). It included all urine samples positive for E. coli collected from adult outpatients. During the study period, 3,443 urinalyses positive for E. coli were studied. In 46% of cases, patients were women. In 64.4% of cases, E. coli were β-lactamase producers. The most frequently diagnosed resistance mechanisms were penicillinase production and sparing third-generation cephalosporins. Isolated E. coli were extended-spectrum β-lactamase (ESBL) producers in 6.1% of cases. Overall, E. coli was susceptible to amoxicillin in 35.9% [95% CI: 34.3–37.5], to amoxicillin/clavulanic acid in 62.2% [95% CI: 60.6–63.9], to cefotaxime in 94% [95% CI: 93.1–94.7], to gentamicin in 92.1% [95% CI: 89.1–92.6], to ofloxacin in 76.8% [95% CI: 75.3–78.2], to trimethoprim/sulfamethoxazole (SXT) in 58.8% [95% CI: 57.1–60.5], to fosfomycin in 99.1% [95% CI: 98.6–99.4], and to nitrofurantoin in 99% of cases [95% CI: 98.6–99.3]. We have observed a gradual decline in the susceptibility profile of E. coli for amoxicillin/clavulanic acid (P <0.001), piperacillin/tazobactam (P = 0.003), and temocillin (P = 0.006). However, susceptibility to ciprofloxacin was increasing (P = 0.001). In contrast, the susceptibility trends for amoxicillin, third-generation cephalosporins, gentamicin, SXT, nitrofurantoin, and fosfomycin remained stable over the 28 quarters of the study. In conclusion, isolated E. coli from outpatient urinalyses showed increased resistance profiles involving penicillinase and ESBL production. Close monitoring and strategies to decrease antibiotic consumption in the community are needed.
Case Report: Salmonella typhi Iliopsoas Abscess with Concomitant Sacroiliitis in a Young Immunocompetent Male: A Rare Case
Authors: Kumar Bhuvanesh , Agarwal Dipasha , Meena Durga Shankar , Kumar Deepak , Sureka Binit 
Published: June 2024

ABSTRACT.
Iliopsoas abscess is an infrequent condition characterized by the collection of pus in the iliopsoas compartment. The prevalence of the disease has been increasing in recent years with the emergence of various comorbidities and risk factors. The availability of newer imaging modalities has also improved the detection of new cases. Salmonellosis is an uncommon etiology in iliopsoas abscess and sacroiliitis. Most cases reported in the literature are associated with Staphylococcus aureus, Streptococci species, and Escherichia coli. Diabetes, hematological malignancies, HIV, and other immunocompromised states are important comorbidities/risk factors for iliopsoas abscess. We report a case of an 18-year-old male who presented with a history of fever and right hip pain for 10 days. Radioimaging revealed right sacroiliitis and iliopsoas abscess. Blood culture revealed pan-sensitive Salmonella typhi. After the prolonged course of antibiotics (intravenous ceftriaxone followed by oral levofloxacin), the patient improved with no further relapse in symptoms. Salmonella typhi should be an important differential of iliopsoas abscess in endemic regions after ruling out the common etiology such as S. aureus and Mycobacterium tuberculosis.
Bacteriological and Serological Investigation of Leptospirosis in Dogs and Pigs in Palau
Authors: Suzuki Ryo , Suzuki Sayaka , Sato Obak Sherman , Alfonso Rush , Kitalong Christopher , Nwe Yin Yin , Takabe Kyosuke , Morita Masatomo , Akeda Yukihiro , Koizumi Nobuo 
Published: June 2024

ABSTRACT.
Leptospirosis is a zoonotic disease caused by the pathogenic spirochaetes of the genus Leptospira. It is a public health concern in the Pacific Islands and is considered endemic in Palau. However, information on the genotypes and serotypes of causative Leptospira spp. in the country is limited. In this study, we isolated leptospires and detected antileptospiral antibodies in dogs and pigs. The isolates were characterized using a serological method and whole-genome sequencing. Leptospira interrogans was isolated from five of the 20 symptomatic dogs and one of the 58 healthy pigs. Their serogroups were identified as Icterohaemorrhagiae and Pyrogenes; however, the serogroup of one isolate could not be determined. Anti-Leptospira antibodies were detected in 14.4% (26/181) of the dogs and 20% (10/50) of the pigs. The reactive serogroups in dogs and pigs were almost identical, except for the Panama serogroup. Core genome multilocus sequence typing revealed that five of the six core genome sequence types (cgSTs) were newly identified in this study. The cgSTs from the serogroup Icterohaemorrhagiae isolates belonged to the same group as the Copenhageni and Icterohaemorrhagiae serovars isolated in other countries, whereas no similar cgSTs were identified in the Pyrogenes or unidentified serogroup strains. We demonstrated a high incidence of canine and porcine leptospirosis and identified new L. interrogans genotypes (cgSTs) circulating in Palau. Further investigations are needed to determine whether dogs and pigs serve as maintenance hosts for newly identified L. interrogans genotypes and whether they pose a risk of leptospirosis transmission to humans.
Professional Inquiry as a Strategy for Language Teacher Development
Authors: Borg Simon , Soltyska Anna , Oldfield-Mariano Melissa , Davis Alan , Friedel Ablanedo Beatriz , Salas Fernandez Paula 
Published: June 2024

Von Oktober 2022 bis März 2023 führte das Zentrum für Fremdsprachenausbildung (ZFA) der Ruhr-Universität Bochum ein Programm zur beruflichen Weiterbildung seiner Lehrkräfte durch. Dieser einleitende Artikel stellt Professional Inquiry (professionelle Unterrichtsuntersuchung) als eine Strategie für die Entwicklung von Lehrkräften vor und beschreibt die Prinzipien, die ihr und somit auch dem ZFA-Projekt zugrunde liegen. Überdies wird im Folgenden ein Überblick über die drei von den Lehrkräften des ZFA durchgeführten Projekte gegeben und dargelegt bzw. erläutert, welchen Nutzen das Projekts für die Lehrkräfte hatte und welchen Herausforderungen sie dabei begegnet sind. Im Anschluss an diesen einleitenden Teil werden die vollständigen Berichte der teilnehmenden Lehrkräfte vorgestellt. Diese Berichte zeigen, wie systematische Unterrichtsuntersuchungen von Lehrenden ihr Verständnis vom Lehren und Lernen vertiefen können. Die Erfahrungen dieses Projekts am ZFA zeigen, dass die professionelle Unterrichtsuntersuchung ein praktikabler Ansatz für die Entwicklung von Sprachlehrern an Universitäten ist.