bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐acetyltransferase 10 facilitates tumorigenesis of diffuse large B‐cell lymphoma by regulating AMPK/mTOR signalling through N4‐acetylcytidine modification of SLC30A9
Authors: Ding Mengfei , Yu Zhuoya , Lu Tiange , Hu Shunfeng , Zhou Xiangxiang , Wang Xin 
Published: July 2024

BackgroundAccumulating studies suggested that posttranscriptional modifications exert a vital role in the tumorigenesis of diffuse large B‐cell lymphoma (DLBCL). N4‐acetylcytidine (ac4C) modification, catalyzed by the N‐acetyltransferase 10 (NAT10), was a novel type of chemical modification that improves translation efficiency and mRNA stability.MethodsGEO databases and clinical samples were used to explore the expression and clinical value of NAT10 in DLBCL. CRISPER/Cas9‐mediated knockout of NAT10 was performed to determine the biological functions of NAT10 in DLBCL. RNA sequencing, acetylated RNA immunoprecipitation sequencing (acRIP‐seq), LC‐MS/MS, RNA immunoprecipitation (RIP)‐qPCR and RNA stability assays were performed to explore the mechanism by which NAT10 contributed to DLBCL progression.ResultsHere, we demonstrated that NAT10‐mediated ac4C modification regulated the occurrence and progression of DLBCL. Dysregulated N‐acetyltransferases expression was found in DLBCL samples. High expression of NAT10 was associated with poor prognosis of DLBCL patients. Deletion of NAT10 expression inhibited cell proliferation and induced G0/G1 phase arrest. Furthermore, knockout of NAT10 increased the sensitivity of DLBCL cells to ibrutinib. AcRIP‐seq identified solute carrier family 30 member 9 (SLC30A9) as a downstream target of NAT10 in DLBCL. NAT10 regulated the mRNA stability of SLC30A9 in an ac4C‐dependent manner. Genetic silencing of SLC30A9 suppressed DLBCL cell growth via regulating the activation of AMP‐activated protein kinase (AMPK) pathway.ConclusionCollectively, these findings highlighted the essential role of ac4C RNA modification mediated by NAT10 in DLBCL, and provided insights into novel epigenetic‐based therapeutic strategies.
Multi‐omics analysis reveals drivers of loss of β‐cell function after newly diagnosed autoimmune type 1 diabetes: An INNODIA multicenter study
Authors: Armenteros Jose Juan Almagro , Brorsson Caroline , Johansen Christian Holm , Banasik Karina , Mazzoni Gianluca , Moulder Robert , Hirvonen Karoliina , Suomi Tomi , Rasool Omid , Bruggraber Sylvaine F. A. , Marcovecchio M. Loredana , Hendricks Emile , Al‐Sari Naba , Mattila Ismo , Legido‐Quigley Cristina , Suvitaival Tommi , Chmura Piotr J. , Knip Mikael , Schulte Anke M. , Lee Jeong Heon , Sebastiani Guido , Grieco Giuseppina Emanuela , Elo Laura L. , Kaur Simranjeet , Pociot Flemming , Dotta Francesco , Tree Tim , Lahesmaa Riitta , Overbergh Lut , Mathieu Chantal , Peakman Mark , Brunak Søren ,   the INNODIA investigators
Published: July 2024

AbstractAimsHeterogeneity in the rate of β‐cell loss in newly diagnosed type 1 diabetes patients is poorly understood and creates a barrier to designing and interpreting disease‐modifying clinical trials. Integrative analyses of baseline multi‐omics data obtained after the diagnosis of type 1 diabetes may provide mechanistic insight into the diverse rates of disease progression after type 1 diabetes diagnosis.MethodsWe collected samples in a pan‐European consortium that enabled the concerted analysis of five different omics modalities in data from 97 newly diagnosed patients. In this study, we used Multi‐Omics Factor Analysis to identify molecular signatures correlating with post‐diagnosis decline in β‐cell mass measured as fasting C‐peptide.ResultsTwo molecular signatures were significantly correlated with fasting C‐peptide levels. One signature showed a correlation to neutrophil degranulation, cytokine signalling, lymphoid and non‐lymphoid cell interactions and G‐protein coupled receptor signalling events that were inversely associated with a rapid decline in β‐cell function. The second signature was related to translation and viral infection was inversely associated with change in β‐cell function. In addition, the immunomics data revealed a Natural Killer cell signature associated with rapid β‐cell decline.ConclusionsFeatures that differ between individuals with slow and rapid decline in β‐cell mass could be valuable in staging and prediction of the rate of disease progression and thus enable smarter (shorter and smaller) trial designs for disease modifying therapies as well as offering biomarkers of therapeutic effect.
A fine‐scale examination of parturition timing in temperate ungulates
Authors: Turnley Matthew T. , Hughes Tabitha A. , Larsen Randy T. , Hersey Kent R. , Broadway Matthew S. , Chitwood M. Colter , Fairbanks W. Sue , Lonsinger Robert C. , McMillan Brock R. 
Published: July 2024

AbstractParturition timing has long been a topic of interest in ungulate research. However, few studies have examined parturition timing at fine scale (e.g., <1 day). Predator activity and environmental conditions can vary considerably with diel timing, which may result in selective pressure for parturition to occur during diel times that maximize the likelihood of neonate survival. We monitored parturition events and early‐life survival of elk (Cervus canadensis) and mule deer (Odocoileus hemionus) in Utah, USA to better understand diel timing of parturition in temperate ungulates. Diel timing of parturition was moderately synchronous among conspecifics and influenced by environmental variables on the date of parturition. For elk, parturition events were most common during the morning crepuscular period and generally occurred later (i.e., closer to 12:00) when a relatively large proportion of the moon was illuminated. For mule deer, parturition events were most common during the diurnal period and generally occurred later (i.e., closer to 15:00) on cold, wet dates. Diel timing of parturition did not influence neonate survival, but larger datasets may be required to verify the apparent lack of influence. Although additional work could evaluate alternative variables that might affect parturition timing, our data provide an improved and finer scale understanding of reproductive ecology and phenology in ungulates.
Hypoalbuminaemia and heart failure: A practical review of current evidence
Authors: Biancucci Marta , Barbiero Riccardo , Pennella Benedetta , Cannatà Antonio , Ageno Walter , Tangianu Flavio , Maresca Andrea Maria , Dentali Francesco , Bonaventura Aldo 
Published: July 2024

AbstractHypoalbuminaemia (serum albumin levels ≤3.5 g/dl) is associated with poor outcomes among patients with heart failure (HF). This narrative review includes original articles and reviews published over the past 20 years and retrieved from PubMed using the following search terms (or their combination): ‘heart failure’, ‘hypoalbuminaemia’, ‘heart failure with reduced ejection fraction’, ‘heart failure with preserved ejection fraction’, ‘all‐cause mortality’, ‘in‐hospital mortality’, ‘hospitalization’, ‘prognosis’. The aims of this review are to provide an overview on the prevalence of hypoalbuminaemia in HF, its impact on clinical outcomes, and potential mechanisms that may suggest future therapeutic strategies. Hypoalbuminaemia is frequent in HF patients, especially among the elderly. However, data about the exact epidemiology of hypoalbuminaemia are scant due to different definitions, and prevalence is estimated between 5% and 70% across the whole spectrum of ejection fraction. Current evidence points to hypoalbuminaemia as a marker of poor outcomes in HF, irrespective of the ejection fraction, and in other cardiovascular diseases. Among patients who suffered from acute coronary syndrome, those with hypoalbuminaemia had an increased risk of new‐onset HF and in‐hospital mortality. Albumin, however, might also play a role in the natural history of such diseases due to its antioxidant, anti‐inflammatory, and antithrombotic properties. Whether albumin supplementation or nutritional support in general would be beneficial in improving clinical outcomes in HF is not completely clear and should be evaluated in adequately designed studies.
Improving the triphenylphosphine/triphenylphosphine oxide (TPP/TPPO)‐based method for the absolute and accurate quantification by FTIR‐ATR of hydroperoxides in oils or lipid extracts
Authors: Wind Juliette , Villeneuve Pierre , Taty Mignon Prince Exaucé , Figueroa‐Espinoza Maria Cruz , Baréa Bruno , Pradelles Rémi , Durand Erwann 
Published: July 2024

AbstractResearch on natural sources of polyunsaturated fatty acids (PUFAs) for both food and nutraceutical prospects has significantly grown in recent years. Some plant oils and lipid extracts contain carotenoids, xanthophylls, sterols, and/or phenolic compounds that can provoke a lipid hydroperoxides (LOOHs) overestimation when quantifying them using nonselective colorimetric assays. Herein, we have optimized a mid‐infrared method using Fourier‐transform infrared spectroscopy coupled with attenuated total reflectance (ATR) to quantify LOOHs in oils or lipid extracts. This method is based on the conversion of triphenylphosphine (TPP) to triphenylphosphine oxide (TPPO) in the presence of hydroperoxides, with a direct assessment of TPPO levels following the formation of an oil film on the ATR crystal's surface, allowing for a low detection limit of 0.5 mmol of LOOH kg−1. The concentration of oil and TPP, as well as the reaction time, were optimized. It was demonstrated that the presence of pigments, unsaponifiable compounds, phenolics, and/or PUFAs on oils, do not disrupt the analysis. Furthermore, the stoichiometry of the TPP/LOOH reaction was examined, confirming the reliability of the method in detecting various forms of hydroperoxides. An accelerated oxidation study was carried out and the hydroperoxide contents measured using TPP/TPPO were found to be comparable to those obtained using the ferric thiocyanate method. Our method offers a fast, simple, robust, and sensitive approach to accurately quantify hydroperoxides, regardless of the chemical composition of oil or lipid extracts.Practical Application: The hydroperoxide assay method outlined in this study allows for the rapid and straightforward detection of hydroperoxides in pure oil matrices. The method's elevated sensitivity facilitates the early identification of oxidation indicators in oils, especially those with significant carotenoid or xanthophyll contents since these compounds may affect the results when using methods based on colorimetric quantification of hydroperoxides. This accurate, precise, and reproducible approach requires only small quantities of test samples and chemical products, making it well suited to routine application in laboratories and industrial environments.
Local nano‐electrode fabrication utilizing nanofluidic and nano‐electrochemical control
Authors: Morikawa Kyojiro , Takeuchi Tomoaki , Kitamori Takehiko 
Published: July 2024

AbstractMiniaturized systems have attracted much attention with the recent advances in microfluidics and nanofluidics. From the capillary electrophoresis, the development of glass‐based microfluidic and nanofluidic technologies has supported advances in microfluidics and nanofluidics. Most microfluidic systems, especially nanofluidic systems, are still simple, such as systems constructed with simple straight nanochannels and bulk‐scale electrodes. One of the bottlenecks to the development of more complicated and sophisticated systems is to develop the locally integrated nano‐electrodes. However, there are still issues with integrating nano‐electrodes into nanofluidic devices because it is difficult to fit the nano‐electrode size into a nanofluidic channel at the nanometer level. In this study, we propose a new method for the fabrication of local nano‐electrodes in nanofluidic devices with nanofluidic and nano‐electrochemistry‐based experiments. An electroplating solution was introduced to a nanochannel with control of the flow and the electroplating reaction, by which nano‐electrodes were successfully fabricated. In addition, a nanofluidic device was available for nanofluidic experiments with the application of 200 kPa. This method can be applied to any electroplating material such as gold and copper. The local nano‐electrode will make a significant contribution to the development of more complicated and sophisticated nanofluidic electrophoresis systems and to local electric detection methods for various nanofluidic devices.
Analyzing water hyacinth plants from two South African rivers for the detection of seven pharmaceuticals and their metabolites
Authors: Himmelsbach Markus , Mlynek Franz , Buchberger Wolfgang , Madikizela Lawrence , Klampfl Christian W. 
Published: July 2024

AbstractWater hyacinth plants (Eichhornia crassipes Mart.) collected from two South African rivers were analyzed in order to investigate their suitability for judging the presence of pharmaceuticals in the water. Thereby, a number of drugs, including amitriptyline, atenolol, citalopram, orphenadrine, lidocaine, telmisartan, and tramadol, could be detected. Particularly for the latter substance, relatively high concentrations (more than 5000 ng g−1 dry plant material) were detected in the water plants. Subsequently, the plant extracts were also screened for drug‐derived transformation products, whereby a series of phase‐one metabolites could be tentatively identified.
Synthesis of FeVO4 nanoparticles using sol‐gel auto‐combustion method and their application in supercapacitors
Authors: Pardeshi Onkar M. , Naeem Sajid , Patil Arun V. 
Published: July 2024

AbstractIron vanadate (FeVO4) nanoparticles (NPs) were synthesized using the sol‐gel auto‐combustion technique, yielding a triclinic nanostructure as revealed by X‐ray diffraction (XRD). The average size, crystalline structure, and morphology of the nanoparticles were analyzed using field emission scanning electron microscopy (FESEM). Energy‐dispersive X‐ray spectroscopy (EDX) was used to investigate the elemental content and purity of the FeVO4 NPs. Fourier transform infrared spectroscopy (FTIR) confirmed the surface stretching frequency of the FeVO4 NPs. Using a doctor blade, the produced FeVO4 NPs were applied to the surface of a stainless steel (SS) substrate. The fabricated electrode was examined using GCD, EIS, and CV techniques. The absorption spectra exhibited strong absorbance in the visible range, with a band gap of 3.43 eV. Additionally, the FeVO4 electrode showed supercapacitor properties, with a maximum specific capacitance of 1151.05 F/g in a 1 M KOH electrolyte at a scan rate of 5 mV/s. These results indicate that the prepared FeVO4 electrode is promising for supercapacitor application due to their excellent electrochemical performance.
Discovery of a novel series of phenylthiazole thioether (sulfone) compounds based on natural thiasporine A as potential candidates for controlling rice fungal and bacterial diseases
Authors: Shi Jinchao , Tian Yao , Zhang Xiaojun , Mao Guoqing , Yin Panpan , Hu Richa , Feng Yapeng , Yu Linhua , Zhu Xiang , Li Junkai 
Published: July 2024

AbstractHalf of the world's population depends on rice for their calories. Protecting rice in the growth period from damage caused by phytopathogens is faced with a great challenge under the frequent extreme climate. To find novel fungicides to control rice diseases, 35 novel phenylthiazole‐1,3,4‐oxadiazole‐thioether (sulfone) derivatives were synthesized and evaluated for their efficacy against destructive fungal and bacterial diseases of rice. Bioassay results demonstrated that most of G‐series compounds possessed excellent antifungal and antibacterial activities. In particular, compounds G1 (EC50 = 2.22 μg/mL, R.s) and G7 (EC50 = 2.76 μg/mL, R.s) showed the most promising antifungal activities in vitro and exhibited superior protective and curative activities against rice sheath blight in vivo compared with commercial carbendazim. Surprisingly, compound G2 exhibited the remarkable antibacterial activity against Xanthomonas oryzae pv. oryzae (Xoo) with an EC50 value of 1.98 μg/mL, and demonstrated superior protective activity (88.08%) than thiodiazole copper (79.39%) against rice bacterial leaf blight at 200 μg/mL. The abovementioned results fully manifested that the phenylthiazole‐1,3,4‐oxadiazole‐sulfone structure, especially compounds G1 and G2, had the potential to develop as commercial agents for controlling rice fungal and bacterial diseases.
Mechanical ventilation management and airway pressure release ventilation practice in acute respiratory distress syndrome: A cross‐sectional survey of intensive care unit clinicians in mainland China
Authors: Lv Yinxia , Jin Xinyang , Dong Meiling , Yang Lan , Wang Bo , Liang Guopeng , Wang Peng , Jing Xiaorong , Yang Yiyi , Yang Yunqin , Wang Zhen , Liao Xuelian , Yang Wei , Zhu Shuo , Lu Mandie , Kang Yan , Zhou Yongfang 
Published: July 2024

AbstractBackgroundAirway pressure release ventilation (APRV) has become increasingly popular for the management of acute respiratory distress syndrome (ARDS); however, its clinical impact remains a topic of debate. Furthermore, there is a gap between the guidelines and the actual clinical practices in mechanical ventilation management for ARDS. This survey aimed to explore the utilization of APRV and mechanical ventilation strategies for ARDS in Chinese intensive care unit (ICU) clinicians.MethodsA comprehensive 34‐item survey was distributed online platforms amongst ICU clinicians across mainland China from June to August 2019.ResultsA total of 420 valid responses were collected, with 57.4% (241) originating from academic hospitals and 42.6% (179) from non‐academic hospitals. Of the respondents, 98.6% (414) recognized the significance of low tidal volume ventilation for ARDS prognosis, 85.2% adhered to a tidal volume below 8 mL/kg predicted body weight, and most (46.4%) selected the initial positive end‐expiratory pressure within the range of 5–10 cmH2O based on experience. Among the respondents, 62.1% (261) reported familiarity with APRV and 41.9% (176) had implemented APRV. Of those who had utilized APRV, 93.2% (164) believed in its effectiveness for ARDS patients and 69.3% (122) advocated for early application of APRV. Substantial variations were noted regarding APRV initiation settings and the preservation of spontaneous breathing during APRV. Academic hospitals exhibited higher usage rates of lung recruitment, neuromuscular blockade, prone ventilation, and acquaintance with and utilization of APRV compared to non‐academic hospitals (all p values ≤ 0.001).ConclusionsOur findings highlight opportunities for improvement in mechanical ventilation management for ARDS, particularly in non‐academic hospitals. Additionally, a significant proportion of clinicians demonstrated limited knowledge of APRV, and there was a lack of consensus on its application. Further training and larger‐scale clinical trials are required to validate the efficacy and utilization of APRV in managing ARDS.
Association of maximum uterine diameter with postoperative complications in laparoscopic supracervical hysterectomy: A retrospective cohort study
Authors: Chu Yu‐Qun , Ding Dah‐Ching 
Published: July 2024

AbstractObjectiveTo determine the maximum uterine diameter threshold associated with an elevated risk of complications following laparoscopic supracervical hysterectomy (LSH).MethodsThis was a retrospective cohort study from a single tertiary referral center. We enrolled patients who underwent LSH for benign indications at our hospital between January 2013 and June 2023. The primary outcome was the occurrence of surgical complications within the 30‐day timeframe of hysterectomy. The covariate included the year of the procedure, patient age, body mass index, parity, American Society of Anesthesiologists classification, comorbidities, history of previous abdominal and pelvic surgery, and preoperative anemia, blood loss, surgical time, hospital stay and pathology. The exclusion criteria comprised those who underwent hysterectomy for malignancy, individuals who underwent total vaginal hysterectomy or laparoscopically assisted vaginal hysterectomy, and those with missing data on uterine maximum diameter, study outcomes, or covariates.ResultsWe included a final sample of 120 patients, revealing a median uterine diameter of 9.12 cm, with 9.2% experiencing complications. The median uterine weight among 40 patients was 275 g. Receiver operating characteristic (ROC) curve analysis suggested a potential cutoff of 11.55 cm for predicting complications, with an area under the ROC curve of 0.67. Multivariate logistic regression confirmed a significant association between uterine diameter exceeding the cutoff and increased complication risk (OR 33.925, 95% CI: 2.294–501.690, P = 0.0103). A correlation (r = 0.762, P < 0.001) between uterine weight and diameter indicated the latter's suitability for preoperative assessment of uterine weight.ConclusionThe maximum uterine diameter with an optimal cutoff of 11.55 cm was associated with increased complication risk.
The bidirectional relationship between benign/malicious envy and subjective well‐being in adolescents: The mediating effect of self‐esteem
Authors: Liu Li , Miao Hualing , He Li , Wang Juan , Guo Cheng 
Published: July 2024

AbstractIntroductionSubjective well‐being, an important index for measuring mental health, is presently declining among junior high school students. Envy, one of their common emotions, is inextricably linked to subjective well‐being. Based on the Dual Envy Theory, our research explores the bidirectional relationship between benign‐malicious envy and subjective well‐being. The mediating role of self‐esteem, as well as the related gender differences, is examined.MethodsChinese middle school students (n = 1566, boys 50.3%, age = 13.96 ± 0.88 years old) were assessed at two time points over a 3‐month interval. Structural equation modeling was used to examine the longitudinal relationships among the variables.Results(1) Cross‐lagged analysis showed a positive bidirectional relationship between benign envy and subjective well‐being and a negative bidirectional relationship between malicious envy and subjective well‐being in the total sample. However, the path from T1 subjective well‐being to T2 malicious envy in boys was not significant. (2) Self‐esteem mediated the relationship between both benign and malicious envy and subjective well‐being among both boys and girls. A Wald chi‐square test showed that T2 self‐esteem was a stronger predictor of T2 benign envy in boys than in girls.ConclusionThe results reveal a virtuous cycle of benign envy and subjective well‐being, and a vicious cycle of malicious envy and subjective well‐being, while emphasizing the role of self‐esteem in this process. Gender differences were also noted. These findings have important implications for improving the subjective well‐being of secondary school students and exploring the positive effects of envy.