bluuebunny/crossref_metadata_2025_split huggingface dataset:
Toward aNOTCH1/FBXW7/RAS/PTEN–Based Oncogenetic Risk Classification of Adult T-Cell Acute Lymphoblastic Leukemia: A Group for Research in Adult Acute Lymphoblastic Leukemia Study
Authors: Trinquand Amélie , Tanguy-Schmidt Aline , Ben Abdelali Raouf , Lambert Jérôme , Beldjord Kheira , Lengliné Etienne , De Gunzburg Noémie , Payet-Bornet Dominique , Lhermitte Ludovic , Mossafa Hossein , Lhéritier Véronique , Bond Jonathan , Huguet Françoise , Buzyn Agnès , Leguay Thibaud , Cahn Jean-Yves , Thomas Xavier , Chalandon Yves , Delannoy André , Bonmati Caroline , Maury Sebastien , Nadel Bertrand , Macintyre Elizabeth , Ifrah Norbert , Dombret Hervé , Asnafi Vahid 
Published: October 2013

PurposeThe Group for Research in Adult Acute Lymphoblastic Leukemia (GRAALL) recently reported a significantly better outcome in T-cell acute lymphoblastic leukemia (T-ALL) harboring NOTCH1 and/or FBXW7 (N/F) mutations compared with unmutated T-ALL. Despite this, one third of patients with N/F-mutated T-ALL experienced relapse.Patients and MethodsIn a series of 212 adult T-ALLs included in the multicenter randomized GRAALL-2003 and -2005 trials, we searched for additional N/K-RAS mutations and PTEN defects (mutations and gene deletion).ResultsN/F mutations were identified in 143 (67%) of 212 patients, and lack of N/F mutation was confirmed to be associated with a poor prognosis. K-RAS, N-RAS, and PTEN mutations/deletions were identified in three (1.6%) of 191, 17 (8.9%) of 191, and 21 (12%) of 175 patients, respectively. The favorable prognostic significance of N/F mutations was restricted to patients without RAS/PTEN abnormalities. These observations led us to propose a new T-ALL oncogenetic classifier defining low-risk patients as those with N/F mutation but no RAS/PTEN mutation (97 of 189 patients; 51%) and all other patients (49%; including 13% with N/F and RAS/PTEN mutations) as high-risk patients. In multivariable analysis, this oncogenetic classifier remained the only significant prognostic covariate (event-free survival: hazard ratio [HR], 3.2; 95% CI, 1.9 to 5.15; P < .001; and overall survival: HR, 3.2; 95% CI, 1.9 to 5.6; P < .001).ConclusionThese data demonstrate that the presence of N/F mutations in the absence of RAS or PTEN abnormalities predicts good outcome in almost 50% of adult T-ALL. Conversely, the absence of N/F or presence of RAS/PTEN alterations identifies the remaining cohort of patients with poor prognosis.
The prevalence of IgG4-related hypophysitis in 170 consecutive patients with hypopituitarism and/or central diabetes insipidus and review of the literature
Authors: Bando Hironori , Iguchi Genzo , Fukuoka Hidenori , Taniguchi Masaaki , Yamamoto Masaaki , Matsumoto Ryusaku , Suda Kentaro , Nishizawa Hitoshi , Takahashi Michiko , Kohmura Eiji , Takahashi Yutaka 
Published: October 2013

ObjectiveThe prevalence and clinical characteristics of IgG4-related hypophysitis remain unclear due to the limited number of case reports. Therefore, in this study, we screened consecutive outpatients with hypopituitarism and/or diabetes insipidus (DI) to estimate its prevalence.MethodsA total of 170 consecutive outpatients with hypopituitarism and/or central DI were screened at Kobe University Hospital for detecting IgG4-related hypophysitis by pituitary magnetic resonance imaging, measuring serum IgG4 concentrations, assessing the involvement of other organs, and carrying out an immunohistochemical analysis to detect IgG4-positive cell infiltration.ResultsAmong the screened cases, 116 cases were excluded due to diagnosis of other causes such as tumors and congenital abnormalities. Additionally, 22 cases with isolated ACTH deficiency were analyzed and were found not to meet the criteria of IgG4-related hypophysitis. The remaining 32 cases were screened and seven were diagnosed with IgG4-related hypophysitis, of which three cases were diagnosed by analyzing pituitary specimens. IgG4-related hypophysitis was detected in 30% (seven of 23 patients) of hypophysitis cases and 4% of all hypopituitarism/DI cases. The mean age at the onset of IgG4-related hypophysitis was 61.8±8.8 years, and the serum IgG4 concentration was 191.1±78.3 mg/dl (normal values 5–105 mg/dl and values in IgG4-related disease (RD) ≥135 mg/dl). Pituitary gland and/or stalk swelling was observed in six patients, and an empty sella was observed in one patient. Multiple co-existing organ involvement was observed in four of the seven patients prior to the onset of IgG4-related hypophysitis.ConclusionThese data suggest that the prevalence of IgG4-related hypophysitis has been underestimated. We should also consider the possibility of the development of hypopituitarism/DI caused by IgG4-related hypophysitis during the clinical course of other IgG4-RDs.
Effects of lidocaine administration via continuous rate infusion on the minimum alveolar concentration of isoflurane in New Zealand White rabbits (Oryctolagus cuniculus)
Authors: Schnellbacher Rodney W. , Carpenter James W. , Mason Diane E. , KuKanich Butch , Beaufrère Hugues , Boysen Courtney 
Published: October 2013

Abstract
Objective—To evaluate the effect of a continuous rate infusion (CRI) of lidocaine on the minimum alveolar concentration (MAC) of isoflurane in rabbits.
Animals—Five 12-month-old female New Zealand White rabbits (Oryctolagus cuniculus).
Procedures—Rabbits were anesthetized with isoflurane. Baseline isoflurane MAC was determined by use of the tail clamp technique. A loading dose of lidocaine (2.0 mg/kg, IV) was administered followed by a CRI of lidocaine at 50 μg/kg/min. After 30 minutes, isoflurane MAC was determined. Another loading dose was administered, and the lidocaine CRI then was increased to 100 μg/kg/min. After 30 minutes, isoflurane MAC was determined again. Plasma samples were obtained for lidocaine analysis after each MAC determination.
Results—Baseline isoflurane MAC was 2.09%, which was similar to previously reported values in this species. Lidocaine CRI at 50 and 100 μg/kg/min induced significant reductions in MAC. The 50 μg/kg/min CRI resulted in a mean plasma lidocaine concentration of 0.654 μg/mL and reduction of MAC by 10.5%. The 100 μg/kg/min CRI of lidocaine resulted in a mean plasma concentration of 1.578 μg/mL and reduction of MAC by 21.7%. Lidocaine also induced significant decreases in arterial blood pressure and heart rate. All cardiopulmonary variables were within reference ranges for rabbits anesthetized with inhalation anesthetics. No adverse effects were detected; all rabbits had an uncomplicated recovery from anesthesia.
Conclusions and Clinical Relevance—Lidocaine administered as a CRI at 50 and 100 μg/kg/min decreased isoflurane MAC in rabbits. The IV administration of lidocaine may be a useful adjunct in anesthesia of rabbits.
Fertility issues in cancer survivorship
Authors: Kort Jonathan D. , Eisenberg Michael L. , Millheiser Leah S. , Westphal Lynn M. 
Published: October 2013

Answer questions and earn CME/CNEBreakthroughs in cancer diagnosis and treatment have led to dramatic improvements in survival and the need to focus on survivorship issues. Chemotherapy and radiotherapy can be gonadotoxic, resulting in impaired fertility. Techniques to help cancer survivors reproduce have been improving over the past decade. Discussion of the changes to a patient's reproductive health after cancer treatment is essential to providing comprehensive quality care. The purpose of this review is to aid in pre‐ and posttreatment counseling, focusing on fertility preservation and other strategies that may mitigate risks to the patient's reproductive, sexual, and overall health. CA Cancer J Clin 2014;64:118–134. © 2013 American Cancer Society.
Zinc Finger Nuclease-Expressing Baculoviral Vectors Mediate Targeted Genome Integration of Reprogramming Factor Genes to Facilitate the Generation of Human Induced Pluripotent Stem Cells
Authors: Phang Rui-Zhe , Tay Felix Chang , Goh Sal-Lee , Lau Cia-Hin , Zhu Haibao , Tan Wee-Kiat , Liang Qingle , Chen Can , Du Shouhui , Li Zhendong , Tay Johan Chin-Kang , Wu Chunxiao , Zeng Jieming , Fan Weimin , Toh Han Chong , Wang Shu 
Published: October 2013

Abstract
Integrative gene transfer using retroviruses to express reprogramming factors displays high efficiency in generating induced pluripotent stem cells (iPSCs), but the value of the method is limited because of the concern over mutagenesis associated with random insertion of transgenes. Site-specific integration into a preselected locus by engineered zinc-finger nuclease (ZFN) technology provides a potential way to overcome the problem. Here, we report the successful reprogramming of human fibroblasts into a state of pluripotency by baculoviral transduction-mediated, site-specific integration of OKSM (Oct3/4, Klf4, Sox2, and c-myc) transcription factor genes into the AAVS1 locus in human chromosome 19. Two nonintegrative baculoviral vectors were used for cotransduction, one expressing ZFNs and another as a donor vector encoding the four transcription factors. iPSC colonies were obtained at a high efficiency of 12% (the mean value of eight individual experiments). All characterized iPSC clones carried the transgenic cassette only at the ZFN-specified AAVS1 locus. We further demonstrated that when the donor cassette was flanked by heterospecific loxP sequences, the reprogramming genes in iPSCs could be replaced by another transgene using a baculoviral vector-based Cre recombinase-mediated cassette exchange system, thereby producing iPSCs free of exogenous reprogramming factors. Although the use of nonintegrating methods to generate iPSCs is rapidly becoming a standard approach, methods based on site-specific integration of reprogramming factor genes as reported here hold the potential for efficient generation of genetically amenable iPSCs suitable for future gene therapy applications.
Where is the magnetic energy for the expansion phase of auroral substorms accumulated?
Authors: Akasofu Syun‐Ichi 
Published: October 2013

AbstractCharacteristics of the ionospheric current system (the directly driven (DD) / unloading (UL) currents) and the power ε during auroral substorms are examined semiquantitatively in terms of input‐output relationship. The study confirms (a) The magnetic energy for the expansion phase is accumulated with the rate of a little more than 1018 erg/s during the growth phase which lasts for about 1 h (because the ionosphere cannot dissipate the input power). (b) The global energy dissipation rate in the ionosphere (including the Joule heat production and the particle precipitation) is more than 3.3 × 1018 erg/s. (c) Thus, the total energy consumption by the ionosphere during the impulsive expansion phase (lasting for about 1 h) is 1.2 × 1022 erg or up to 1023 erg for medium substorms. (d) However, it is shown that even the total magnetic energy in the magnetotail at a distance between 10 RE and 20 RE (6.5 × 1021 ergs = 6.5 × 1014 J) cannot supply the energy for the expansion phase. This estimate is consistent with the most recent satellite observations (4.3 × 1013 J (= 4.3 × 1020 ergs)). Thus, the following conclusions may be applicable for medium intensity substorms: (i) The major part of the magnetic energy for the expansion phase must be accumulated within a distance of 10 RE and be released almost totally during the expansion phase (because the duration of the growth phase and the expansion phase is about the same). (ii) This suggests that there is an upper limit of the energy which the magnetosphere can accumulate even at distances less than 10 RE. Further, it is confirmed that: (a) The expansion phase is characterized by a single cell current system UL (not a twin cell current system DD which is driven by the earthward plasma convection from the magnetotail). (b) The UL current is independent of the DD current, requiring a different origin. (c) During the recovery phase, the UL current is absent and the DD current approximately follows ε (no accumulation of the energy), because the ionosphere is capable of dissipating the power even if the power is moderate). (d) Based on these results, as an example, one possible chain of processes within 10 RE is proposed as the release process of the accumulated magnetic energy by taking into account the fact that the frozen‐in field condition is observed to be broken down.
One‐pot method for obtaining hydrophilic tetracycline‐imprinted particles via precipitation polymerization in ethanol
Authors: Zhao Chunyan , Dai Jiangdong , Zhou Zhiping , Dai Xiaohui , Zou Yongli , Yu Ping , Zou Tianbian , Li Chunxiang , Yan Yongsheng 
Published: October 2013

ABSTRACTIn this study, we used a green, one‐pot method to synthesize hydrophilic molecularly imprinted polymers (MIPs) via the precipitation polymerization of hydrophilic monomers in ethanol. The as‐prepared materials were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, dynamic light scattering, and water contact angle measurements (27.3 ± 0.1°). As compared to the imprinting and nonimprinting processes, tetracycline (TC), as a template molecule, had an important effect on the morphology of the MIPs, and the possible mechanism is discussed in detail. We also discuss the effects of the parameters on the binding performance as determined by batch adsorption experiments in pure water. The adsorption capacity increased with increasing concentration and temperature at an optimum pH of 5.0. The Langmuir isotherm fitted the data better, with a maximal concentration of 45.75 μmol/g at 318 K. The kinetic properties of the MIPs (within 3.0 h) toward TC were analyzed with pseudo‐first‐order and pseudo‐second‐order kinetic equations and the intraparticle diffusion model. The MIPs exhibited specific recognition toward TC, and other competitive antibiotics were used as references. All of the results indicate that the MIPs exhibited a large adsorption capacity and great specific recognition for TC. The high affinity to TC of the MIPs, with its fast and easy fabrication, provides them with potential applications in the selective separation of the TC antibiotics from an aqueous environment. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40071.
Small‐Peptide‐Based Organogel Kit: Towards the Development of Multicomponent Self‐Sorting Organogels
Authors: Afrasiabi Rouzbeh , Kraatz Heinz‐Bernhard 
Published: October 2013

AbstractThe results presented here highlight the extremely useful nature of ultra‐short peptides as building blocks in the development of smart multicomponent supramolecular devices. A facile bottom‐up strategy for the synthesis of a small library of stimuli‐responsive smart organogelators has been proposed based on the predictive self‐assembly of ultra‐short peptides. More importantly, the narcissistic self‐sorting of the gelators has been evaluated as a simple method for the efficient co‐assembly of a donor–acceptor dual‐component gel, allowing the investigation of possible future applications of similar systems in the development of a supramolecular photo‐conversion device. Interestingly, it was observed that the self‐organization of the components can lead to highly ordered systems in which discrimination between compatible and non‐compatible building blocks directs the effective organization of the chromophores and gives rise to the formation of an excited‐state complex with exciplex‐like emission. The current report may prove important in the development of organogel‐based multicomponent smart devices.
Effect of exercise on patient specific abdominal aortic aneurysm flow topology and mixing
Authors: Arzani Amirhossein , Les Andrea S. , Dalman Ronald L. , Shadden Shawn C. 
Published: October 2013

SUMMARYComputational fluid dynamics modeling was used to investigate changes in blood transport topology between rest and exercise conditions in five patient‐specific abdominal aortic aneurysm models. MRI was used to provide the vascular anatomy and necessary boundary conditions for simulating blood velocity and pressure fields inside each model. Finite‐time Lyapunov exponent fields and associated Lagrangian coherent structures were computed from blood velocity data and were used to compare features of the transport topology between rest and exercise both mechanistically and qualitatively. A mix‐norm and mix‐variance measure based on fresh blood distribution throughout the aneurysm over time were implemented to quantitatively compare mixing between rest and exercise. Exercise conditions resulted in higher and more uniform mixing and reduced the overall residence time in all aneurysms. Separated regions of recirculating flow were commonly observed in rest, and these regions were either reduced or removed by attached and unidirectional flow during exercise, or replaced with regional chaotic and transiently turbulent mixing, or persisted and even extended during exercise. The main factor that dictated the change in flow topology from rest to exercise was the behavior of the jet of blood penetrating into the aneurysm during systole. Copyright © 2013 John Wiley & Sons, Ltd.
The Two Faces of Hydrogen‐Bond Strength on Triple AAA–DDD Arrays
Authors: Lopez Alfredo Henrique Duarte , Caramori Giovanni Finoto , Coimbra Daniel Fernando , Parreira Renato Luis Tame , da Silva Éder Henrique 
Published: October 2013

AbstractSystems that are connected through multiple hydrogen bonds are the cornerstone of molecular recognition processes in biology, and they are increasingly being employed in supramolecular chemistry, specifically in molecular self‐assembly processes. For this reason, the effects of different substituents (NO2, CN, F, Cl, Br, OCH3 and NH2) on the electronic structure, and consequently on the magnitude of hydrogen bonds in triple AAA–DDD arrays (A=acceptor, D=donor) were evaluated in the light of topological [electron localization function (ELF) and quantum theory of atoms in molecules (QTAIM)], energetic [Su–Li energy‐decomposition analysis (EDA) and natural bond orbital analysis (NBO)], and geometrical analysis. The results based on local H‐bond descriptors (geometries, QTAIM, ELF, and NBO) indicate that substitutions with electron‐withdrawing groups on the AAA module tend to strengthen, whereas electron‐donating substituents tend to weaken the covalent character of the AAA–DDD intermolecular H‐bonds, and also indicate that the magnitude of the effect is dependent on the position of substitution. In contrast, Su–Li EDA results show an opposite behavior when compared to local H‐bond descriptors, indicating that electron‐donating substituents tend to increase the magnitude of H‐bonds in AAA–DDD arrays, and thus suggesting that the use of local H‐bond descriptors describes the nature of H bonds only partially, not providing enough insight about the strength of such H bonds.
Temporal variations in the linkage between the net ecosystem exchange of water vapour and CO2over boreal forests in eastern Siberia
Authors: Kotani Ayumi , Kononov Alexander V. , Ohta Takeshi , Maximov Trofim C. 
Published: October 2013

ABSTRACTThis study investigated evapotranspiration and CO2exchange, observed by the eddy covariance method, over two larch‐dominated forests, in the middle part of the Lena basin, eastern Siberia. Compared with the Spasskaya Pad (SP) forest, the Elgeeii (EG) forest had more plant biomass and a soil texture that allowed for larger water retention. Observations in three growing seasons revealed that the two sites had similar seasonal patterns of meteorological conditions and almost the same total evapotranspiration; however, the net and gross uptakes of CO2at the EG forest were 1·61 and 1·31 times those at the SP forest, respectively. The difference in aboveground biomass was probably responsible for the higher productivity at EG. Less aboveground biomass would result in less transpiration by trees at SP, which would be compensated for in total evapotranspiration by soil evaporation and transpiration by the forest floor vegetation. It is likely that forest transpiration is commonly optimized to the same level of evapotranspiration under similar meteorological conditions through differences in the contributions of evapotranspiration from the forest floor vegetation and the soil water availability. Water use efficiency (WUE) displayed different patterns, reflecting the seasonal variations in each flux. Seasonal sequences in the WUE at EG indicated a midsummer depletion, which is commonly observed in forest ecosystems under a broad climate. Daily variation corresponding to the vapour pressure deficit and the cloud cover conditions (reduced solar radiation) was also observed. In contrast, WUE was relatively invariable at the seasonal and daily scales at SP. Copyright © 2013 John Wiley & Sons, Ltd.
Tandem Synthesis of 9,10‐Dihydro‐9,10‐diboraanthracenes via Elusive ortho‐Lithiated Phenylboronates
Authors: Luliński Sergiusz , Smętek Jaromir , Durka Krzysztof , Serwatowski Janusz 
Published: October 2013

AbstractA simple general protocol is reported for the synthesis of functionalised 9,10‐dihydroxy‐9,10‐dihydro‐9,10‐diboraanthracenes by treatment of the appropriate substituted (2‐halophenyl)boronates with tBuLi. The reaction apparently occurs through the formation of ortho‐lithiated phenylboronates. These elusive species possess a trivalent Lewis‐acidic boron atom in the vicinity of a strongly nucleophilic lithiated carbon atom. The results of DFT calculations indicate that Br/Li exchange is kinetically favored over nucleophilic alkylation of the boron atom, which contrasts with the behaviour of the sterically less hindered nBuLi. The results are consistent with lower yields of 9,10‐dihydro‐9,10‐diboraanthracenes obtained by using the latter reagent. Subsequent steps of the cascade reaction sequence involve trapping of the reactive lithium/boron intermediate in situ with the starting boronate, followed by a second halogen/lithium exchange and final ring closure. Isolation of 9,10‐dihydroxy‐9,10‐dihydro‐9,10‐diboraanthracenes was further aided by their complexation with 8‐hydroxyquinoline.
Hydroxy‐Directed Cyclopropanation of Esters: Synthesis of Trisubstituted Cyclopropanols
Authors: Astashko Dmitry , Cha Jin Kun , Rao Nagavaram Narsimha , Parida Bibhuti Bhusan 
Published: October 2013

AbstractThe Kulinkovich cyclopropanation of esters with disubstituted homoallylic alcohols is described for the preparation of 1,2,3‐trisubstituted cyclopropanols. Central to the successful implementation is the generation in situ of a temporary alkoxy tether between a homoallylic alcohol and an alkoxytitanium species to override unfavorable steric factors.
HRM Systems for Knowledge Workers: Differences Among Top Managers, Middle Managers, and Professional Employees
Authors: Krausert Achim 
Published: October 2013

AbstractThis theoretical study contributes to the debate in the field of strategic HRM on whether HRM systems should differ across employee groups within the firm. It explores differences in the efficacy of two HRM systems—high‐involvement HR and internal labor market systems—across top management teams, middle managers, and professional employees. In addition, it examines factors that may prevent the adoption of effective HRM systems for these groups of knowledge workers. A model is proposed depicting how four job‐level moderators influence the HRM‐performance relationship and how these moderators apply to each of the three employee groups. Short‐term performance pressures and favoritism in staffing are proposed as factors that may hinder the adoption of effective HRM systems. Finally, HRM systems for different employee groups are classified based on their gains potential and barriers to implementation, yielding four categories of strategic HRM activity metaphorically labeled low‐hanging fruits, high‐hanging fruits, peanuts, and barren land. © 2013 Wiley Periodicals, Inc.