bluuebunny/crossref_metadata_2025_split huggingface dataset:
TWIST1 Expression in Breast Cancer Cells Facilitates Bone Metastasis Formation
Authors: Croset Martine , Goehrig Delphine , Frackowiak Agnieszka , Bonnelye Edith , Ansieau Stéphane , Puisieux Alain , Clézardin Philippe 
Published: March 2014

ABSTRACT

The transcription factor TWIST1 induces epithelial-mesenchymal transition and/or escape to the oncogenic-induced failsafe program, facilitating the intravasation of breast cancer cells in the systemic circulation and their dissemination to the lungs. Its involvement in breast cancer bone metastasis is unknown. To address this question, human osteotropic MDA-MB-231/B02 breast cancer cells were stably transfected with a Tet-inducible vector encoding for TWIST1, whose expression was specifically repressed in the presence of doxycycline (dox). The intra-arterial inoculation of transfectants expressing TWIST1 in immunodeficient mice substantially increased the extent of osteolytic lesions in these animals, being 50% larger than that of animals bearing mock-transfected tumors, as determined by radiography. This difference was accompanied by a sharp reduction of the bone volume (indicating a higher bone destruction) and a twofold increase in the tumor volume compared with mice bearing mock-transfected tumors, as determined by histomorphometry. Importantly, the suppression of TWIST1 expression in MDA-MB-231/B02 cells in the presence of dox abolished the stimulatory effect of TWIST1 on bone metastasis formation in vivo. Additionally, examination of the bone marrow from untreated and dox-treated animals on day 7 after tumor cell inoculation, at which time there was no evidence of radiographic osteolytic lesions, revealed that the number of tumor cell colonies that were recovered from the bone marrow of untreated mice was dramatically increased compared with that of dox-fed animals. In vitro, TWIST1 expression promoted tumor cell invasion and enhanced microRNA 10b (miR-10b) expression, a proinvasive factor, but was dispensable for growth of tumor cells. In vivo, the repression of miR-10b substantially decreased the presence of TWIST1-expressing breast cancer cells in the bone marrow. Overall, these results establish that TWIST1 facilitates breast cancer bone metastasis formation through a mechanism dependent of miR-10b, which leads to increase tumor burden and bone destruction. © 2014 American Society for Bone and Mineral Research.
Overlapping and Continued Alendronate or Raloxifene Administration in Patients on Teriparatide: Effects on Areal and Volumetric Bone Mineral Density—The CONFORS Study
Authors: Muschitz Christian , Kocijan Roland , Fahrleitner-Pammer Astrid , Pavo Imre , Haschka Judith , Schima Wolfgang , Kapiotis Stylianos , Resch Heinrich 
Published: March 2014

Abstract

Nine month teriparatide (TPTD) monotherapy followed by co-administration of raloxifene (RAL) or alendronate (ALN) for another nine 9 months resulted in incremental bone mineral density (BMD) increase. The aim of this study was to investigate the effects of continued antiresorptive treatments for 12 months in the extension phase. Postmenopausal women (n = 125) with severe osteoporosis on ongoing TPTD treatment for 9 months were randomized into three open-label groups for another 9 months: ALN (70 mg/week, n = 41), RAL (60 mg/d, n = 37) in addition to TPTD or no additional medication (n = 47) except Ca and vitamin D. After discontinuation of TPTD the respective antiresorptives were continued for a further 12 months, while patients in the TPTD monotherapy group received Ca and vitamin D. Amino-terminal propeptide of type I procollagen (P1NP) and cross-linked C-telopeptide (CTX), areal and volumetric BMD at the lumbar spine (LS) and hip were assessed. ALN resulted in continued BMD increase in LS (4.3 ± 1.5%; mean ± SD), femoral neck (4.2 ± 1.6%) and total hip (4 ± 1.6%; p &lt; 0.001 for all), while RAL was only effective at the LS (2.4 ± 1.7%, p &lt; 0.001) but no changes at the femoral neck (0.4 ± 1.4%) or total hip (−0.8 ± 1.5%) were observed. Cortical bone only increased in the ALN group (femoral neck 6.7 ± 2.7% and −1.3 ± 2.5%; total hip 13.8 ± 2.9% and −2.3 ± 2.5% for ALN and RAL, p &lt; 0.001 for all; respectively). Analyzing the entire 30 months of therapy, the ALN group revealed the largest BMD increase in all regions. Our results suggest that the addition of ALN to ongoing TPTD and continuing ALN after TPTD was stopped may be beneficial for patients in terms of areal and volumetric BMD increase. Further research is warranted to determine the optimal timing of the initiation of the combination treatment, the respective antiresorptive medication and the potential benefit of this BMD increase regarding fracture prevention. © 2014 American Society for Bone and Mineral Research
Ankylosing Spondylitis Is Associated With an Increased Risk of Vertebral and Nonvertebral Clinical Fractures: A Population-Based Cohort Study
Authors: Muñoz-Ortego Juan , Vestergaard Peter , Rubio Josep Blanch , Wordsworth Paul , Judge Andrew , Javaid M Kassim , Arden Nigel K , Cooper Cyrus , Díez-Pérez Adolfo , Prieto-Alhambra Daniel 
Published: March 2014

ABSTRACT

The objective of this work was to study the associations between ankylosing spondylitis (AS) and clinical vertebral and nonvertebral fractures. Data from a large population-based public health database in Spain, Sistema d'Informació per al Desenvolupament de l'Investigació en Atenció Primària (SIDIAP), were used in this parallel cohort study. All participants registered in SIDIAP on January 1, 2006, were screened to identify those with a diagnosis of AS. Five age-matched, gender-matched, and general practice surgery–matched controls were selected for each patient with AS. All participants were followed until December 31, 2011, transfer out date, or death date. Fractures during this time were classified as vertebral or nonvertebral. Adjustment was made for potential confounders (tobacco smoking, alcohol consumption, body mass index, and use of oral steroids). Of 4,920,353 eligible patients in SIDIAP, 6474 AS patients with matched controls (n = 32,346) were available. A higher proportion of patients with AS versus controls had clinical vertebral (0.86% versus 0.41%) and nonvertebral (3.4% versus 2.7%) fractures. Adjusted Cox regression models showed an increased risk of clinical vertebral (hazard ratio [HR] 1.93; 95% confidence interval [CI], 1.39 to 2.68; p &lt; 0.001) and nonvertebral (HR 1.19; 95% CI, 1.02 to 1.39; p = 0.03) fractures among patients with AS. However, the observed increased risks were apparent only in those not on regular nonsteroidal anti-inflammatory drugs (NSAIDs). There were no interactions with inflammatory bowel disease, psoriasis, or previous back pain. Patients with AS are at increased risk of vertebral and nonvertebral clinical fractures, independently of various risk factors. Regular use of NSAIDs appears to eliminate the excess fracture risk related to AS, but the mechanisms involved are unknown. © 2014 American Society for Bone and Mineral Research
Determination of Herbicide Difenzoquat Methyl Sulfate in Citruses and Baby Juices by Kinetic‐Spectrophotometric Method and HPLC Method
Authors: Pecev‐Marinković Emilija T. , Grahovac Zora M. , Mitić Snežana S. , Pavlović Aleksandra N. , Rašić Mišić Ivana D. , Mitić Milan N. 
Published: March 2014

AbstractThe purpose of this paper is to present a new kinetic‐spectrophotometric method which involve inexpensive equipment and which can be applied by all analyst who have to determine difenzoquate methyl sulfate (DFQ) residues in citruses and baby juices. The method is based on the inhibited effect of DFQ on the oxidation of sulfanile acid (SA) by hydrogen peroxide in phosphate buffer in presence Cu(II) ion. The reaction was monitored spectrophotometrically by measuring the increase in absorbance of the reaction product at 370 nm. The proposed inhibited method permits determination of DFQ over the range 0.36 to 1.80 μg/mL and 1.80 to 7.20 μg/mL, with quantification limit of 0.184 μg/mL. The relative standard deviations are 0.73‐2.90% for the concentration interval of DFQ 1.80‐0.36 μg/mL. The method was successfully applied to determination of DFQ residues in citruses and baby juices. The HPLC method is used to verify the results. The results obtained for the same samples by the two methods are quite comparable.
Controlling pH in biological depuration of industrial wastewater to enable micropollutant removal using a further advanced oxidation process
Authors: Rivas Ibáñez Gracia , Casas López José Luis , Esteban García Belén , Sánchez Pérez José Antonio 
Published: March 2014

AbstractBACKGROUNDThe accumulation of bicarbonates during the biological treatment of high organic strength wastewater has a negative impact on the remaining micropollutant removal through chemical oxidation due to its scavenging effect on hydroxyl radicals. In this study, a lab‐scale sequencing batch reactor (SBR) was operated at circumneutral pH to avoid inorganic carbon accumulation, followed by Fenton and photo‐Fenton processes to treat an agro‐food industrial effluent with acetamiprid as a model pollutant at 100 µg L−1. A cost analysis of the operation strategy was performed.RESULTSThe pH control did not affect SBR efficiency (98% organic carbon biodegradation) but gave rise to different inorganic carbon concentrations in the effluents: 75.32, 10.81 and 5.7 mg L−1 at pH 8.0, 7.0 and 6.5, respectively. Both Fenton and photo‐Fenton resulted in complete acetamiprid removal at pH 6.5 and 7.0 with high iron dosage (up to 80 mg L−1) but only photo‐Fenton achieved this at acidic pH with 20 mg Fe L−1.CONCLUSIONTo remove micropollutant from biotreated wastewater by Fenton processes it is highly recommended to control pH at circumneutral values during the biotreatment. The reactant cost analysis showed a reduction of ∼80% to remove the micropollutant, even with photo‐Fenton being carried out at pH 2.8. © 2014 Society of Chemical Industry
N‐doped TiO2/s‐PS aerogels for photocatalytic degradation of organic dyes in wastewater under visible light irradiation
Authors: Vaiano Vincenzo , Sacco Olga , Sannino Diana , Ciambelli Paolo , Longo Simona , Venditto Vincenzo , Guerra Gaetano 
Published: March 2014

AbstractBACKGROUNDHeterogeneous photocatalysis for the degradation of non‐biodegradable organic contaminants in wastewater is an alternative to traditional treatment techniques. Since the applications developed employ photocatalysts in powder form, a main disadvantage remains related to the recovery of photocatalysts from the treated wastewater, resulting in damage and shorter lifetime for the recirculation pumps. Structured photocatalysts offer a valid solution to simplify the process; however, they have to guarantee light transmission, long‐term stability under different operating conditions and under mechanical stress.RESULTSThe photocatalytic performances of N‐doped TiO2 nanocomposite syndiotactic polystyrene aerogels (Nt‐sPS) were analyzed in the presence of visible light. A widely used model pollutant, methylene blue (MB), was chosen to verify the photocatalytic performance. After about 180 min of irradiation, on Nt‐sPS, the conversion of MB reached 30%, higher than that obtained (16%) when the photocatalyst is suspended as powder in the liquid reaction medium.CONCLUSIONN‐doped TiO2, obtained by direct nitration during the hydrolysis of titanium tetraisopropoxide with ammonia solutions, was immobilized in s‐PS aerogels, yielding high activity in the removal of methylene blue under visible light irradiation, in comparison with N‐doped TiO2 dispersed as powder in solution. © 2014 Society of Chemical Industry
Clinical trial registries are of minimal use for identifying selective outcome and analysis reporting
Authors: Norris Susan L. , Holmer Haley K. , Fu Rongwei , Ogden Lauren A. , Viswanathan Meera S. , Abou‐Setta Ahmed M. 
Published: March 2014

ObjectiveThis study aimed to examine selective outcome reporting (SOR) and selective analysis reporting (SAR) in randomized controlled trials (RCTs) and to explore the usefulness of trial registries for identifying SOR and SAR.Study Design and SettingWe selected one “index outcome” for each of three comparative effectiveness reviews (CERs) of pharmacotherapy and extracted data on this outcome from trial registries and from study publications.ResultsAmong 50 RCTs published since 2005 and reporting the index outcome, only 50% were listed in registries; 90% of RCTs were assessed as having SOR or SAR. The index outcome in the registry was different from that in the publication in 75% of trials in two CERs, and not specified at all in the third. Reported outcomes and analyses were not consistent between the publication's methods section and the results section in 33% and 46% of the two CERs where the index outcome was a benefit. There were no statistically significant predictors of SOR and SAR in our small sample where some predictors lacked variability.ConclusionThe SOR and SAR were frequent in this pilot study, and the most common type of SOR was the publication of outcomes that were not pre‐specified. Trial registries were of little use in identifying SOR and of no use in identifying SAR. Copyright © 2014 John Wiley & Sons, Ltd.
Evaluation of toxicological impacts on Sprague–Dawley rat by successively low dose of aflatoxin B1
Authors: Wei Wei , Li Rui , He Liang , Wang Xu , Ji Xiaofeng , Zhou Yu 
Published: March 2014

AbstractBACKGROUNDAflatoxins (AFs) are a group of potent mycotoxins that frequently pollute agro‐products and animal feeds. For complex reasons, the regulatory limits of AFs differ from nation to nation. In this study, feedstuffs contaminated with various concentrations of aflatoxin B1 (AFB1) corresponding to AF regulatory limits of major agro‐product importing and exporting nations were tested in Sprague–Dawley rats, and the toxicological results were evaluated.RESULTSDuring the trial period, no poisoning behavior was observed and no animals died. No significant differences in body weight were observed between AFB1‐contaminated and control rats. However, various negative impacts on liver and kidney functions were observed in AFB1‐contaminated groups, and these negative impacts were positively correlated with AFB1 concentrations. At concentrations of 4–50 µg kg−1, AFB1 tolerance was observed for the liver after 15 days of testing, but this tolerance was not observed for the kidney.CONCLUSIONLow concentrations of AFB1 still induced obvious toxicological impacts on Sprague–Dawley rats, and the negative effects were aggravated by increasing the AFB1 dose and feeding time. Therefore strict AF regulatory limits are suggested for food safety administration, especially for the low‐income nations with higher hepatitis B virus prevalence. © 2014 Society of Chemical Industry
Effects of the dietary incorporation of olive leaves on growth performance, digestibility, blood parameters and meat quality of growing pigs
Authors: Paiva‐Martins Fátima , Ribeirinha Teresa , Silva Aníbal , Gonçalves Rita , Pinheiro Victor , Mourão José L , Outor‐Monteiro Divanildo 
Published: March 2014

AbstractBACKGROUNDIn a preliminary study the oxidative stability and tocopherol content of pork meat were shown to be improved by olive leaf (OL) feed supplementation at 50 and 100 g kg−1. However, growth performance was affected negatively. Therefore the objective of the present study was to assess the influence of OL supplementation at a lower level on feed digestibility, growth performance and meat quality.RESULTSPigs were fed a basal diet (control), a basal diet with 25 g OL kg−1 (OL2.5) or a basal diet with 50 g OL kg−1 (OL5). The incorporation of OL significantly decreased growth rates (P = 0.010) and backfat thickness (P = 0.035) and increased feed/gain ratio (P = 0.032) in the OL5 group. Feed/gain ratio increased more for females (P = 0.001). The incorporation of OL decreased the crude fat (P = 0.006) and protein (P = 0.037) digestibility of both OL diets. Nevertheless, OL was effective in increasing the tocopherol content of meat (P = 0.009). However, meat from pigs fed the OL diets showed similar conjugated diene content, pH and colour parameters to that from pigs fed the control diet, even after 6 days of storage at 4 °C.CONCLUSIONThe data indicate that olive leaves may be included in pig diets at 25 g kg−1 in order to improve the tocopherol content of meat without excessively compromising growth performance. © 2014 Society of Chemical Industry
Investigating rapid eye movement sleep without atonia in Parkinson's disease using the rapid eye movement sleep behavior disorder screening questionnaire
Authors: Bolitho Samuel J. , Naismith Sharon L. , Terpening Zoe , Grunstein Ron R. , Melehan Kerri , Yee Brendon J. , Coeytaux Alessandra , Gilat Moran , Lewis Simon J.G. 
Published: March 2014

ABSTRACTRapid eye movement (REM) sleep behavior disorder (RBD) is frequently observed in patients with Parkinson's disease (PD). Accurate diagnosis is essential for managing this condition. Furthermore, the emergence of idiopathic RBD in later life can represent a premotor feature, heralding the development of PD. Reliable, accurate methods for identifying RBD may offer a window for early intervention. This study sought to identify whether the RBD screening questionnaire (RBDSQ) and three questionnaires focused on dream enactment were able to correctly identify patients with REM without atonia (RWA), the neurophysiological hallmark of RBD. Forty‐six patients with PD underwent neurological and sleep assessment in addition to completing the RBDSQ, the RBD single question (RBD1Q), and the Mayo Sleep Questionnaire (MSQ). The REM atonia index was derived for all participants as an objective measure of RWA. Patients identified to be RBD positive on the RBDSQ did not show increased RWA on polysomnography (80% sensitivity and 55% specificity). However, patients positive for RBD on questionnaires specific to dream enactment correctly identified higher degrees of RWA and improved the diagnostic accuracy of these questionnaires. This study suggests that the RBDSQ does not accurately identify RWA, essential for diagnosing RBD in PD. Furthermore, the results suggest that self‐report measures of RBD need to focus questions on dream enactment behavior to better identify RWA and RBD. Further studies are needed to develop accurate determination and quantification of RWA in RBD to improve management of patients with PD in the future. © 2014 International Parkinson and Movement Disorder Society
Loss of ALDH18A1 function is associated with a cellular lipid droplet phenotype suggesting a link between autosomal recessive cutis laxa type 3A and Warburg Micro syndrome
Authors: Handley Mark T. , Mégarbané André , Meynert Alison M. , Brown Stephen , Freyer Elisabeth , Taylor Martin S. , Jackson Ian J. , Aligianis Irene A. 
Published: March 2014

AbstractAutosomal recessive cutis laxa type 3A is caused by mutations in ALDH18A1, a gene encoding the mitochondrial enzyme ∆1‐pyrroline‐5‐carboxylate synthase (P5CS). It is a rare disorder with only six pathogenic mutations and 10 affected individuals from five families previously described in the literature. Here we report the identification of novel compound heterozygous missense mutations in two affected siblings from a Lebanese family by whole‐exome sequencing. The mutations alter a conserved C‐terminal domain of the encoded protein and reduce protein stability as determined through Western blot analysis of patient fibroblasts. Patient fibroblasts exhibit a lipid droplet phenotype similar to that recently reported in Warburg Micro syndrome, a disorder with similar features but hitherto unrelated cellular etiology.
Insights from QM/MM Modeling the 3D Structure of the 2009 H1N1 Influenza A Virus Neuraminidase and Its Binding Interactions with Antiviral Drugs
Authors: Wang Yeng‐Tseng , Chen Yu‐Ching 
Published: March 2014

AbstractControl of drug release through the inhibition of neuraminidase‐1 has been identified as a potential target for the treatment of H1N1 influenza; however, the drug binding mode of neuraminidase is not yet completely understood. In this work, we propose a model for a neuraminidase‐1 complex based on four known X‐ray structures of drug/neuraminidase‐1 complexes. Specifically, H1N1 neuraminidase‐1 complexed with 4 drugs (zanamivir, laninamivir, laninamivir octanoate and oseltamivir) was first investigated using a combined quantum mechanical and molecular mechanical (QM/MM) approach. Based on these structures, a model for the H1N1 neuraminidase‐1 complex was proposed and simulated using the same computational protocol. Implications to drug/H1N1 neuraminidase‐1 binding modes are discussed. From our simulations, the predicted binding free energies of the four drugs are in good agreement with the experimental results, with the correlation coefficient being 0.84.
Theoretical Studies on Pyrazolo[3,4‐d]pyrimidine Derivatives as Potent Dual c‐Src/Abl Inhibitors Using 3D‐QSAR and Docking Approaches
Authors: Zeng Guo Hua , Fang Dan Qing , Wu Wen Juan , Wang Ju Ping , Xie Wen Guo , Ma Shao Jie , Wu Jing Heng , Shen Yong 
Published: March 2014

AbstractIn recent years, the development of dual or multi‐targeted inhibitors has captured extensive attention of research for treating of malignancies. In this paper, three‐dimensional quantitative structure‐activity relationship and docking studies were performed on 87 pyrazolo[3,4‐d]pyrimidines as dual Src/Abl inhibitors. The appropriate binding orientations and conformations of these compounds interacting with both Src and Abl kinases were revealed by docking studies, and the established optimum CoMFA models yielded q2=0.856, R2=0.966 for Src and q2=0.869, R2=0.974 for Abl, and the best CoMSIA models gave q2=0.877, R2=0.979 for Src and q2=0.885, R2=0.982 for Abl. Systemic external validations further confirm the satisfactory predictive power of these models, producing R2pred values of 0.872 and 0.865 for Src, 0.876 and 0.867 for Abl, r2m values of 0.832 and 0.928 for Src, 0.838 and 0.904 for Abl, respectively. In addition, through a comparison between 3D‐QSAR contour maps and docking results, it is revealed that the hydrophobic and electrostatic interactions of compounds play significant roles for the inhibitory activity against both Src and Abl kinases. Some structural factors influencing the activities of these compounds were discussed in detail. The key amino acids impacting the receptor‐ligand interactions have been identified. These theoretical results can offer useful references for designing novel potential dual Src/Abl inhibitors.
Highly sensitive refractive index measurement based an a thinned fiber taper
Authors: Du Chengliang , Yang Xianhui 
Published: March 2014

AbstractA thinned fiber taper (TFT) with high refractive index (RI) sensitivity has been fabricated by a fusion splicer. The TFT has a total length of about 1.3 mm and a waist diameter of less than 10 μm. Simulations of the TFT have been implemented and the RI experiments show a remarkable sensitivity. The highest RI sensitivities of 6360 nm/RI unit (RIU) and 3850 nm/RIU in the RI ranges of 1.409–1.423 and 1.333–1.353, respectively, have been obtained on two TFTs with different taper waist diameters. The RI dynamic ranges of the TFTs also have been discussed. © 2014 Wiley Periodicals, Inc. Microwave Opt Technol Lett 56:1054–1057, 2014
A cavity‐backed spiral slot antenna with wide axial ratio beamwidth for GPS system
Authors: Bao Xiulong L. , Ammann M. J. 
Published: March 2014

AbstractA monofilar spiral slot antenna with a cavity‐backed reflector is presented, which can achieve the performance of the wide axial ratio beamwave for global positioning system (GPS) system. It comprises an arrangement of a single printed spiral slots fed by a 50‐Ω microstrip line. By using cavity‐backed covering the slot structure, a wide axial ratio beamwidth performance is achieved in the GPS L1 frequency. The angle with less than 3‐dB axial ratio at 1.575 GHz in the YoZ plane is about 215°. The measured results show that the HPBW beamwidths at 1.575 GHz are 100° in the YoZ plane, respectively. The peak gain is 5.5 dBic. © 2014 Wiley Periodicals, Inc. Microwave Opt Technol Lett 56:1050–1054, 2014