bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Reaction of Grignard Reagents with Diisopropylaminoborane. Synthesis of Alkyl, Aryl, Heteroaryl and Allyl Boronic Acids from Organo(diisopropyl)aminoborane by a Simple Hydrolysis.
Authors: Bailey Christopher L. , Murphy Chris L. , Clary Jacob W. , Eagon Scott , Gould Naomi , Singaram Bakthan 
Published: April 2013

AbstractBoronic acids (III) are synthesized by Grignard reaction of aminoborane (I), whereas boronic acids (V) are synthesized by reaction of (I) with in situ formed Grignard reagents (Barbier conditions).
ChemInform Abstract: Bis‐E‐chloromethylidene Derivatives of 4‐Thio‐ and 4‐Selenomorpholinamines
Authors: Martynov A. V. , Makhaeva N. A. , Larina L. I. , Amosova S. V. 
Published: April 2013

Abstractsynthesis and spectroscopic characterization
ChemInform Abstract: Toward the Synthesis of Modified Carbohydrates by Conjugate Addition of Propane‐1,3‐dithiol to α,β‐Unsaturated Ketones.
Authors: Valdersnes Stig , Apeland Ingrid , Flemmen Guri , Sydnes Leiv K. 
Published: April 2013

AbstractMeONa‐mediated addition of dithiol (II) to alkynes (I) is accompanied by an intramolecular cyclization to afford spiro compounds (III), which can be regarded as partly modified furanose derivatives.
Retinorecipient areas in the diurnal murine rodent Arvicanthis niloticus: A disproportionally large superior colliculus
Authors: Gaillard Frédéric , Karten Harvey J. , Sauvé Yves 
Published: April 2013

AbstractThe Nile grass rat (Arvicanthis niloticus) has a high proportion of cone photoreceptors (∼30–40%) compared with that in the common laboratory mouse and rat (∼1–3%) and may prove a preferable murine model with which to study cone‐driven information processing in retina and primary visual centers. However, other than regions involved in circadian control, little is known about the retinorecipient structures in this rodent. We undertook a detailed analysis of the retinal projections as revealed after intravitreal injection of the anterograde tracer cholera toxin subunit B. Retinal efferents were evaluated in 45 subcortical structures. Contralateral projections were always dominant. Major contralateral inputs consisted of the suprachiasmatic nucleus, dorsolateral geniculate nucleus (dLGN), intergeniculate leaflet, ventral geniculate nucleus (magnocellular part), lateroposterior thalamic nucleus, all six pretectal nuclei, superficial layers of the superior colliculus (SC), and the main nuclei of the accessory optic system. Terminals from the contralateral eye were also localized in an unnamed field rostromedial to the dLGN as well as in the subgeniculate thalamic nucleus. Ipsilateral inputs were found mainly in the suprachiasmatic nucleus, dLGN, intergeniculate leaflet, internal sector of the magnocellular part of the ventral geniculate nucleus, olivary pretectal nucleus, and SC optic layer. Retinal afferents were not detected in the basal forebrain or the dorsal raphe nucleus. Morphometric measurements revealed that the superficial layers of the SC are disproportionately enlarged relative to other retinorecipient regions and brain size compared with rats and mice. We suggest that this reflects the selective projection of cone‐driven retinal ganglion cells to the SC. J. Comp. Neurol. 521:1699–1726, 2013. © 2012 Wiley Periodicals, Inc.
Molecule‐Induced Peroxide Homolysis
Authors: Turrà Natascia , Neuenschwander Ulrich , Hermans Ive 
Published: April 2013

AbstractThe homolytic cleavage of peroxide bonds, leading to the formation of free radicals, plays an important role in the (spontaneous) oxidation of a wide variety of hydrocarbons in the presence of oxygen. Such aerobic oxidations can be desired (e.g. for industrially applied autoxidations) or undesired (e.g. food deterioration). In this contribution we provide experimental and computational evidence for a molecule‐induced homolytic dissociation mechanism between alkyl peroxide and compounds featuring weakly bonded H atoms such as (di)unsaturated hydrocarbons.
From Coordination Polyhedra to Molecular Environment and Back – Interplay between Coordinate and Hydrogen Bonds in Two Polymorphs of a Cobalt Complex
Authors: Ananyev Ivan V. , Nefedov Sergey E. , Lyssenko Konstantin A. 
Published: April 2013

AbstractTwo crystalline modifications of a cobalt(II) benzoate complex containing substituted pyrazole ligands are investigated. The polymorphs are characterized by different intermolecular bonding as well as by different metal‐atom environments. A thorough analysis of the chemical bonding patterns by means of Bader's “Atoms in Molecules” theory reveals the significant role of N–H···O hydrogen bonds in charge transfer within the molecule and in stabilization of unfavorable [4+1] cobalt polyhedra. Specifically, an estimation of the interaction energies according to an Espinosa correlation scheme shows the significant contribution of H bonds to the stabilization energy of the molecular conformations.
Electrochemical and Catalytic Studies of a Manganese(III)Complex with a Tetradentate Schiff‐Base Ligand Encapsulated in NaY Zeolite
Authors: Kuźniarska‐Biernacka Iwona , Rodrigues Otilia , Carvalho Maria Alice , Parpot Pier , Biernacki Krzysztof , Magalhães Alexandre Lopes , Fonseca Antonio Mauricio , Neves Isabel Correia 
Published: April 2013

AbstractThe manganese(III) complex with a Schiff‐base salen‐type ligand (1,5‐bis{[(1E)‐(2‐hydroxyphenyl)methylene]amino}‐1H‐imidazole‐4‐carbonitrile) has been encapsulated in the nanopores of a Y zeolite by using two different methodologies, the flexible ligand and in situ complex preparation methods. Cyclic voltammetry studies revealed that the neat complex undergoes reversible oxidation in dmf, which has been attributed to the MnII/III redox couple, whereas the two heterogeneous catalysts show different electrochemical behaviour in aqueous medium. The encapsulated and non‐encapsulated homogeneous MnIIIsalen complexes were screened as catalysts for olefin oxidation by using tBuOOH as the oxygen source in different solvents. Under the optimized conditions, the catalysts exhibited moderate activity. Both heterogeneous catalysts catalysed the oxidation of cyclohexene with tBuOOH, primarily to give the allylic oxidation products. These catalysts were found to be reusable after the catalytic cycle, but with some loss of activity. A DFT study confirmed the distortion of the complex in the zeolite cage, the main difference being observed for the bonded chloride.
A well‐balanced stable generalized Riemann problem scheme for shallow water equations using adaptive moving unstructured triangular meshes
Authors: Zhou Feng , Chen Guoxian , Noelle Sebastian , Guo Huaicheng 
Published: April 2013

SUMMARYWe propose a well‐balanced stable generalized Riemann problem (GRP) scheme for the shallow water equations with irregular bottom topography based on moving, adaptive, unstructured, triangular meshes. In order to stabilize the computations near equilibria, we use the Rankine–Hugoniot condition to remove a singularity from the GRP solver. Moreover, we develop a remapping onto the new mesh (after grid movement) based on equilibrium variables. This, together with the already established techniques, guarantees the well‐balancing. Numerical tests show the accuracy, efficiency, and robustness of the GRP moving mesh method: lake at rest solutions are preserved even when the underlying mesh is moving (e.g., mesh points are moved to regions of steep gradients), and various comparisons with fixed coarse and fine meshes demonstrate high resolution at relatively low cost. Copyright © 2013 John Wiley & Sons, Ltd.
Nutritional status, food intake, and dysphagia in long‐term survivors with head and neck cancer treated with chemoradiotherapy: A cross‐sectional study
Authors: van den Berg Manon G. A. , Rütten Heidi , Rasmussen‐Conrad Ellen L. , Knuijt Simone , Takes Robert P. , van Herpen Carla M. L. , Wanten Geert J. A. , Kaanders Johannes H. A. M. , Merkx Matthias A. W. 
Published: April 2013

BackgroundThe aim of this study was to evaluate nutritional status, food intake, and dysphagia in long‐term head and neck cancer survivors.MethodsThirty‐two patients with stage III–IV head and neck cancer treated by chemoradiotherapy were invited to evaluate nutritional status (malnutrition, relative weight loss), food intake (food modification; quality), and dysphagia.ResultsAt a median follow up of 44 months, 6 of 32 patients were at risk for malnutrition. Women (p = .049) and patients with high body mass index before treatment (p = .024) showed more weight loss. None of the 32 patients could eat a “full diet.” Six patients used nutritional supplements/tube feeding. Low dysphagia‐related quality of life scores were significantly correlated to increased food modification (r = 0.405; p = .024).ConclusionsNutritional advice in patients with head and neck cancer is still necessary years after chemoradiation and should focus on nutritional status, food modification, and quality, in accord with recommended food groups. © 2013 Wiley Periodicals, Inc. Head Neck 36: 60–65, 2014
Longitudinal study on the correlations of thyroid antibody and thyroid hormone levels after radiotherapy in patients with nasopharyngeal carcinoma with radiation‐induced hypothyroidism
Authors: Lin Zhixiong , Chen Linxing , Fang Yusen , Cai Aiqun , Zhang Tuodan , Wu Vincent W. C. 
Published: April 2013

BackgroundThe purpose of this study was to evaluate the correlations of thyroid antibody and thyroid hormone in postradiotherapy (post‐RT) patients with nasopharyngeal carcinoma (NPC).MethodsSerum thyroid‐stimulating hormone (TSH), free triiodothyronine (fT3), free thyroxine (fT4), thyroperoxidase antibody (anti‐TPO), and thyroglobulin antibody (anti‐TG) levels of 55 patients with NPC were taken before RT and at 3, 6, 12, and 18 months after RT. Patients with hypothyroidism at 18 months after RT and those with normal thyroid function were categorized into the hypo and normal groups, respectively. The thyroid antibody levels were compared between the 2 groups, and the correlations of thyroid antibody and thyroid hormone were evaluated for the hypo group.ResultsThe anti‐TPO and anti‐TG of the hypo group were higher than the normal group. In the hypo group, there was a positive correlation (r = 0.51) between TSH and anti‐TG and a negative correlation between fT4 and anti‐TPO (r = −0.55).ConclusionThere were correlations of the thyroid antibody and thyroid hormone levels in patients with radiation‐induced hypothyroidism. © 2013 Wiley Periodicals, Inc. Head Neck 36: 171–175, 2014
Preferential expansion of umbilical cord blood‐derived CD34‐positive cells on human leukemia inhibitory factor transgenic feeder cells cultured on regenerated silk fibroin film
Authors: Yu Xin , Miao Jingcheng , Xia Wei , Gu Zong‐jiang 
Published: April 2013

AbstractIn vitro expansion of transplantable hematopoietic stem cells (HSCs) is a very promising approach for different clinical applications. We have recently developed a new culture system that facilitates in vitro expansion of transplantable cord blood HSCs. In our study, we constructed a recombinant adenovirus Ad‐GFP/human leukemia inhibitory factor (hLIF) expressing hLIF. The hLIF gene was delivered into human embryo lung fibroblast cell line WI‐38 via infection with Ad‐GFP/hLIF. Then, the transgenic cells were cultured on regenerated silk fibroin (SF) films as feeder layer cells for expansion of cord blood CD34+ cells. Our results showed that the hLIF transgenic WI‐38 cells cultured on SF could express hematopoiesis‐related cytokines at higher levels compared with control groups. The hLIF‐expressing feeder layer cells cultured on SF in combination with cytokines more efficiently expanded CD34+ cells and CD34+CD38− cells. The percentages of adhesion molecules on the expanded CD34+ cells in transgenic feeder layer cells cultured on SF were higher than those of control groups. Interestingly, the migration rate assessed by transwell assay was also significantly higher than those of control groups, which suggests that transgenic feeder layer cells cultured on SF has powerful ability to maintain the homing capacity of expanded CD34+ cells. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Dynamic competitive adsorption of bone‐related proteins on calcium phosphate ceramic particles with different phase composition and microstructure
Authors: Wang Jing , Zhang Huijie , Zhu Xiangdong , Fan Hongsong , Fan Yujiang , Zhang Xingdong 
Published: April 2013

AbstractThe biocompatibility and bioactivity of biomaterials used for hard tissue repair are closely related to their adsorption capacities for bone‐related proteins. In the present study, three types of calcium phosphate (CaP) ceramic particles with different phase composition or microstructure were fabricated, and their protein adsorption abilities were investigated by a self‐made device under the simulated dynamic physiological circumstance. The results of X‐ray diffraction, field emission scanning electron microscopy, mercury penetration test, and nitrogen sorption test showed that the irregular hydroxyapatite (HA) ceramic particles obtained by conventional drying and sintering (named as HA‐C) had fewer micropores and lower specific surface area (SSA) than did the spherical HA or biphasic calcium phosphate (BCP) ceramic particles made by spray drying and sintering (named as HA‐S and BCP‐S, respectively). The dynamic protein adsorption study proved that both the phase composition and microstructure of CaP ceramic particles affected their adsorption capacities for those bone‐related proteins. The spherical HA‐S and BCP‐S particles with abundant micropores and high SSA showed higher adsorption of serum proteins, including fibronectin and vitronectin, than the irregular HA‐C did. On the other hand, in spite of the relatively high concentration of bovine serum albumin (BSA) in the binary bone morphogenetic protein 2 (BMP‐2)/BSA solution, BMP‐2 adsorption on the three CaP ceramic particles increased with the increase in its initial concentration. Similarly, HA‐S and BCP‐S particles had a larger amount of the adsorbed BMP‐2 per gram solid than HA‐C did. Therefore, it could be believed that the difference of various CaP ceramics in the phase composition and microporous structure would affect their binding capacity for those bone‐related proteins and thus lead to their difference in osteoinduction. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Nuclear localization of the actin regulatory protein palladin in sertoli cells
Authors: Niedenberger Bryan A. , Chappell Vesna K. , Kaye Evelyn P. , Renegar Randall H. , Geyer Christopher B. 
Published: April 2013

SUMMARYIn the testis, F‐actin structures are involved in spermatid nuclear remodeling and cytoplasm reduction, maintenance of the blood–testis barrier, support of the spermatogonial stem cell niche, and release of spermatids into the tubular lumen. To gain a better understanding of actin regulation in Sertoli–germ cell interactions, we investigated the expression of the Palladin (Palld) gene, which encodes a widely expressed phosphoprotein that localizes to actin‐rich cytoplasmic structures, including focal adhesions, cell–cell junctions, podosomes, and stress fibers, and serves as a molecular scaffold to bundle actin fibers. In germ cells, PALLD was concentrated along the tubulin‐ and F‐actin‐containing cytoplasmic manchette that forms adjacent to the elongating spermatid nucleus during spermiogenesis. To our surprise, PALLD relocated from the cytoplasm to the nucleus of Sertoli cells in the juvenile testis, coincident with the onset of puberty, and this localization was maintained in the adult. We provide evidence that the 140 kDa isoform of PALLD predominates in Sertoli cells, and that it is apparently cleaved, with the C‐terminus localizing to the nucleus while the N‐terminus remains cytoplasmic. We investigated the nuclear localization of the C‐terminus of PALLD and found that it is regulated by a putative nuclear export signal. These results provide the foundation for future work employing Sertoli cell‐ and spermatid‐specific Palld‐knockout mice to study diverse roles of PALLD as both a nuclear‐actin regulatory protein and as a potential regulator of manchette formation during spermatogenesis. Mol. Reprod. Dev. 80: 403–413, 2013. © 2013 Wiley Periodicals, Inc.
The effects of the WT1 gene on apoptosis and development‐related gene expression in porcine kidney fibroblasts and swine testis cells
Authors: An Peipei , Yin Yupeng , Fan Anran , Tan Wentao , Gao Fei , Ma Kuiying , Tang Bo , Zhang Xueming , Li Ziyi 
Published: April 2013

AbstractThe Wilm's tumor 1 gene (WT1) encodes zinc finger proteins that function as tumor suppressors, and play important roles in the development of the genito‐urinary system and other organs. Its precise function in the development of porcine tissues and organs, which is an attractive transplantation resource for certain human diseases, is still unclear. Here, we sought to define the role of WT1 in porcine kidney and testis tissues using porcine kidney fibroblasts (PKFs) and swine testis (ST) cells as in vitro models, both of which express WT1. The recombinant plasmids pLV3‐WT1 shRNA and pIRES2‐WT1‐EGFP were constructed to respectively down‐ and up‐regulate the WT1 gene in porcine cells. The role of WT1 in cell proliferation was investigated by RT‐PCR, immunocytochemical staining, apoptosis analysis, and Western blot. The pLV3‐WT1 shRNA dramatically reduced WT1 expression at both the transcription and protein levels. The down‐regulation of WT1 directly led to early cell apoptosis, and changes in Sf1, Sox9, and Gdnf gene expression in PKFs and ST cells. In contrast, up‐regulation of WT1 gave no obvious phenotype in ST cells. Our results demonstrate that WT1 is essential for the survival of PKFs and ST cells because it regulates apoptosis‐ and development‐related genes in the cells; however, no obvious effect was observed when WT1 was over‐expressed. Mol. Reprod. Dev. 80: 414–425, 2013. © 2013 Wiley Periodicals, Inc.
Gene Expression Analysis of Bovine Oocytes With High Developmental Competence Obtained From FSH‐Stimulated Animals
Authors: Labrecque Rémi , Vigneault Christian , Blondin Patrick , Sirard Marc‐André 
Published: April 2013

SUMMARYRecent progress in the ovarian stimulation protocol used for bovine in vitro maturation and fertilization, especially through optimization of the follicle‐stimulating hormone (FSH) withdrawal period (“coasting”) after ovarian pre‐treatment with FSH, has significantly improved blastocyst outcome. Despite this important success, the underlying factors leading to improved oocyte quality have not yet been identified. The aim of this project was to compare the transcriptome of germinal vesicle‐stage oocytes collected from FSH‐stimulated cows after various coasting periods (20, 44, 68, and 92 hr) to determine which transcripts were accumulated or depleted during the rise and fall of competence. Oocytes from each coasting period were compared to the three other times (optimal conditions, 44 and 68 hr; under‐matured, 20 hr; and over‐matured, 92 hr) per animal, allowing each cow to be its own control (24 collections). Microarray analysis revealed that between 5 and 338 transcripts were significantly different across the six comparisons, with an important longitudinal modulation in terms of gene expression profile. Not surprisingly, as the transcriptional activity decreased in these oocytes, several transcripts that are significantly modulated during coasting are related to RNA processing functions, as shown by functional analysis. Ingenuity Pathway Analysis also highlighted another important function: the control of chromosome segregation. The results presented here indicate that the quality gained with the optimal coasting time does not last, and also suggests a possible mechanism of control by transcript degradation that could be implicated if the oocyte is not ovulated at the right time. Mol. Reprod. Dev. 80: 428–440, 2013. © 2013 Wiley Periodicals, Inc.
Proteomics of human seminal plasma: Identification of biomarker candidates for fertility and infertility and the evolution of technology
Authors: Milardi Domenico , Grande Giuseppe , Vincenzoni Federica , Castagnola Massimo , Marana Riccardo 
Published: April 2013

AbstractProteomics is a research area that has developed rapidly in the last decade. It studies the large‐scale characterization of the full protein components of a cell, a tissue, or a biological fluid. In the last decade, clinical proteomics has developed new technology and bioinformatics useful in identifying molecular markers of pathology; the next decade might be the era of proteomics. Seminal plasma (SP) represents a good sample for proteomic analysis in the evaluation of male fertility/infertility. SP is an acellular fluid conglomerate, comprised of contributions from the epididymis and accessory sexual glands. Human SP contains many proteins that are important to the successful fertilization of the oocyte by the spermatozoa. Proteomic studies have identified numerous seminal‐specific proteins, and recent reports have provided a further understanding of their function with respect to male fertility. Upon further validation, these proteins may be useful in the clinical distinction between fertility and infertility. This article reviews the proteomic methods, such as one dimensional polyacrylamide gel electrophoresis (1D–PAGE), two‐dimensional polyacrylamide gel electrophoresis (2D–PAGE), and mass spectrometry (MS), employed to detect human SP markers involved in fertility and infertility. As such, proteomic studies will help the development of new techniques to identify novel biomarkers for a better clinical diagnosis and treatment of male infertility. Mol. Reprod. Dev. 80: 350–357, 2013. © 2013 Wiley Periodicals, Inc.
Factors affecting the clinical use of non‐invasive prenatal testing: a mixed methods systematic review
Authors: Skirton Heather , Patch Christine 
Published: April 2013

ABSTRACTNon‐invasive prenatal testing has been in clinical use for a decade; however, there is evidence that this technology will be more widely applied within the next few years. Guidance is therefore required to ensure that the procedure is offered in a way that is evidence based and ethically and clinically acceptable. We conducted a systematic review of the current relevant literature to ascertain the factors that should be considered when offering non‐invasive prenatal testing in a clinical setting. We undertook a systematic search of relevant databases, journals and reference lists, and from an initial list of 298 potential papers, identified 11 that were directly relevant to the study. Original data were extracted and presented in a table, and the content of all papers was analysed and presented in narrative form. Four main themes emerged: perceived attributes of the test, regulation and ethical issues, non‐invasive prenatal testing in practice and economic considerations. However, there was a basic difference in the approach of actual or potential service users, who were very positive about the benefits of the technology, compared with other research participants, who were concerned with the potential moral and ethical outcomes of using this testing method. Recommendations for the appropriate use of non‐invasive prenatal testing are made. © 2013 John Wiley & Sons, Ltd.