bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential Translocation of the Fatty Acid Transporter, FAT/CD36, and the Glucose Transporter, GLUT4, Coordinates Changes in Cardiac Substrate Metabolism During Ischemia and Reperfusion
Authors: Heather Lisa C. , Pates Katharine M. , Atherton Helen J. , Cole Mark A. , Ball Daniel R. , Evans Rhys D. , Glatz Jan F. , Luiken Joost J. , Griffin Julian L. , Clarke Kieran 
Published: August 2013

Background—
Fatty acid and glucose transporters translocate between the sarcolemma and intracellular compartments to regulate substrate metabolism acutely. We hypothesised that during ischemia fatty acid translocase (FAT/CD36) would translocate away from the sarcolemma to limit fatty acid uptake when fatty acid oxidation is inhibited.

Methods and Results—

Wistar rat hearts were perfused during preischemia, low-flow ischemia, and reperfusion, using
3
H-substrates for measurement of metabolic rates, followed by metabolomic analysis and subcellular fractionation. During ischemia, there was a 32% decrease in sarcolemmal FAT/CD36 accompanied by a 95% decrease in fatty acid oxidation rates, with no change in intramyocardial lipids. Concomitantly, the sarcolemmal content of the glucose transporter, GLUT4, increased by 90% during ischemia, associated with an 86% increase in glycolytic rates, 45% decrease in glycogen content, and a 3-fold increase in phosphorylated AMP-activated protein kinase. Following reperfusion, decreased sarcolemmal FAT/CD36 persisted, but fatty acid oxidation rates returned to preischemic levels, resulting in a 35% decrease in myocardial triglyceride content. Elevated sarcolemmal GLUT4 persisted during reperfusion; in contrast, glycolytic rates decreased to 30% of preischemic rates, accompanied by a 5-fold increase in intracellular citrate levels and restoration of glycogen content.

Conclusions—
During ischemia, FAT/CD36 moved away from the sarcolemma as GLUT4 moved toward the sarcolemma, associated with a shift from fatty acid oxidation to glycolysis, while intramyocardial lipid accumulation was prevented. This relocation was maintained during reperfusion, which was associated with replenishing glycogen stores as a priority, occurring at the expense of glycolysis and mediated by an increase in citrate levels.
Race Influences the Safety and Efficacy of Spironolactone in Severe Heart Failure
Authors: Vardeny Orly , Cavallari Larisa H. , Claggett Brian , Desai Akshay S. , Anand Inder , Rossignol Patrick , Zannad Faiez , Pitt Bertram , Solomon Scott D. 
Published: August 2013

Background—
The incidence of hyperkalemia caused by mineralocorticoid receptor antagonists may vary by race, but whether race influences efficacy of mineralocorticoid receptor antagonists in heart failure (HF) is unknown.

Methods and Results—

We assessed hyperkalemia and outcomes in African Americans (AAs; n=120) and non-AAs (n=1543; white 93%) with New York Heart Association (NYHA) class III or IV HF and left ventricular dysfunction who were randomized to spironolactone, titrated to 25 or 50 mg daily or placebo, in the Randomized Aldactone Evaluation Study (RALES). AA participants were significantly younger, less likely to have an ischemic HF pathogenesis, more likely to be NYHA functional class IV, and more likely to have a higher estimated glomerular filtration rate and heart rate, less hypertension, diabetes mellitus, or history of myocardial infarction compared with non-AA participants. Potassium increased with spironolactone in non-AAs (4.29±0.5–4.55±0.49 mmol/L) but not in AAs (4.32±0.54–4.31±0.49 mmol/L; race by treatment interaction,
P
=0.03) during the first month and remained higher throughout the trial. Compared with AAs, non-AAs were more likely to attain maximal spironolactone dose (13.9% versus 5.8%;
P
=0.04) and had higher rates of hyperkalemia (potassium>5.5 mmol/L; 9.7% versus 4.2%;
P
<0.046), as well as lower rates of hypokalemia (potassium<3.5 mmol/L; 5.6% versus 17.9%;
P
<0.001). After adjustment for differences in baseline characteristics and achieved study drug dose, spironolactone reduced the combined end point of death or hospitalization for HF in non-AAs (hazard ratio, 0.63; 95% confidence interval, 0.55–0.73) but not in AAs (hazard ratio, 1.07; 95% confidence interval, 0.67–1.71;
P
value for interaction=0.032).

Conclusions—
AAs with HF exhibited less hyperkalemia and more hypokalemia with spironolactone compared with non-AAs and seemed to derive less clinical benefit. These hypothesis-generating findings suggest that safety and efficacy of mineralocorticoid receptor antagonists may differ by race.
Atherosclerosis Susceptibility Loci Identified in an Extremely Atherosclerosis‐Resistant Mouse Strain
Authors: Rowlan Jessica S. , Li Qiongzhen , Manichaikul Ani , Wang Qian , Matsumoto Alan H. , Shi Weibin 
Published: August 2013

Background

C3H/HeJ (C3H) mice are extremely resistant to atherosclerosis, especially males. To understand the underlying genetic basis, we performed quantitative trait locus (
QTL
) analysis on a male F
2
(the second generation from an intercross between 2 inbred strains) cohort derived from an intercross between C3H and C57
BL
/6 (B6) apolipoprotein E–deficient (
Apoe
−/−
) mice.

Methods and Results

Two hundred forty‐six male F
2
mice were started on a Western diet at 8 weeks of age and kept on the diet for 5 weeks. Atherosclerotic lesions in the aortic root and fasting plasma lipid levels were measured. One hundred thirty‐four microsatellite markers across the entire genome were genotyped. Four significant
QTL
s on chromosomes (Chr) 2, 4, 9, and 15 and 4 suggestive loci on Chr1, Chr4, and Chr7 were identified for atherosclerotic lesions. Unexpectedly, the C3H allele was associated with increased lesion formation for 2 of the 4 significant
QTL
s. Six loci for high‐density lipoprotein (
HDL
), 6 for non‐
HDL
cholesterol, and 3 for triglycerides were also identified. The
QTL
for atherosclerosis on Chr9 replicated
Ath29
, originally mapped in a female F
2
cohort derived from B6 and C3H
Apoe
−/−
mice. This locus coincided with a
QTL
for
HDL
, and there was a moderate, but statistically significant, correlation between atherosclerotic lesion sizes and plasma
HDL
cholesterol levels in F
2
mice.

Conclusions

These data indicate that most atherosclerosis susceptibility loci are distinct from those for plasma lipids except for the Chr9 locus, which exerts effect through interactions with
HDL
.
Comparing the Roles of Barotropic versus Baroclinic Feedbacks in the Atmosphere’s Response to Mechanical Forcing
Authors: Barnes Elizabeth A. , Thompson David W. J. 
Published: August 2013

Abstract
Do barotropic or baroclinic eddy feedbacks dominate the atmospheric circulation response to mechanical forcing?
To address this question, barotropic torques are imposed over a range of latitudes in both an idealized general circulation model (GCM) and a barotropic model. The GCM includes both baroclinic and barotropic feedbacks. The barotropic model is run in two configurations: 1) only barotropic feedbacks are present and 2) a baroclinic-like feedback is added by allowing the stirring region to move with the jet. The relationship between the latitude of the forcing and the response is examined by systematically shifting the torques between the tropics and the pole. The importance of the mean state is investigated by varying the position of the control jet.
Five main findings are presented: 1) Barotropic feedbacks alone are capable of producing the structure of the GCM response to mechanical forcing but are not capable of accounting for its full magnitude. 2) Baroclinic processes generally increase the magnitude of the response but do not strongly influence its structure. 3) For a given forcing, the largest response in all model configurations occurs 5°–10° poleward of the forcing latitude. 4) The maximum response occurs when the forcing is located approximately 10° poleward of the control jet. 5) The circulation response weakens as the mean jet is found at higher latitudes in all model configurations.
Laboratory Investigation of the Impact of Lateral Spreading on Buoyancy Flux in a River Plume
Authors: Yuan Yeping , Horner-Devine Alexander R. 
Published: August 2013

AbstractThe relationship between lateral spreading and mixing in stratified gravity currents is investigated by comparing laterally confined and unconfined currents in a series of laboratory experiments. The vertical turbulent buoyancy flux is determined using a control volume approach with velocity and density fields derived from combined particle image velocimetry (PIV) and planar-laser-induced fluorescence (PLIF). Lateral spreading is determined in the unconfined experiments based on plan-view imaging using the optical thickness method (OTM). The authors find that lateral spreading dramatically modifies the plume structure; the spreading plume layer consists of approximately linear density and velocity profiles that extend to the surface, whereas the channelized plume profiles are uniform near the surface. Lateral spreading decreases the average plume density relative to laterally confined currents with similar inflow conditions. However, the local turbulent buoyancy flux in the spreading experiments is approximately equal to that in the confined experiments. This apparent paradox is resolved when the plume areas are taken into account. The total mixing integrated over the horizontal plume area is significantly higher in the spreading experiments. Thus, the experiments suggest that spreading does not appreciably alter the turbulent mixing processes at the base of the plume. However, it significantly increases the area over which this mixing occurs and, through this mechanism, increases the net dilution of river water at a fixed distance from the river mouth. Finally, the authors hypothesize that the spreading does not significantly increase the local turbulent buoyancy flux because spreading occurs preferentially near the surface, whereas buoyancy flux is greatest in the core of the current.
An illustration of the optimization of combined cooling heating and power systems using genetic algorithm
Authors: Wang Jiangjiang , Yang Kun , Zhang Xutao , Shi Guohua , Fu Chao 
Published: August 2013

The performances of combined cooling, heating and power (CCHP) system are greatly dependent on its design, operation strategy and thermal and electric demands. This paper illustrates how the use of a genetic algorithm can provide speedy optimization, by applying it to two styles of buildings operated in different operation strategies. The primary energy consumptions of CCHP system following electric demand management (EDM) and thermal demand management (TDM) are firstly analyzed respectively. Then, sixteen hypothetical buildings are constructed to represent various energy demands. Primary energy saving (PES), annual total cost saving (ATCS), and CO2 emission reduction (CO2ER), are weighted to evaluate the integrated performances of CCHP system in comparison to separation production system. Finally, the optimized CCHP system for sixteen scenarios using GA are compared. Practical application: This paper provides an optimization design method for CCHP system. The performance analysis of CCHP systems running different operation modes for different buildings is believed by the authors to contribute to a significant guide for the fundamental design of CCHP systems. Although sensitivity to a number of other important design considerations such equipment performance, possible future changes in operating conditions, changes to the price or carbon intensity of grid-supplied energy etc are not addressed, the conclusions present a simple and effective direction and the proposed optimization algorithm and the evaluation method for CCHP system can be extended to other buildings.
The Microvascular Volume of the Achilles Tendon Is Increased in Patients With Tendinopathy at Rest and After a 1-Hour Treadmill Run
Authors: Pingel Jessica , Harrison Adrian , Simonsen Lene , Suetta Charlotte , Bülow Jens , Langberg Henning 
Published: August 2013

Background: Achilles tendinopathy (AT) is initiated asymptomatically and is therefore often discovered at a very late stage. Purpose: To elucidate whether the microvascular volume (MV) of the Achilles tendon is elevated in patients with AT compared with healthy controls during pre-exercise rest, after acute exercise, and 24 hours after exercise. Additionally, this study investigated the muscle activation pattern of the gastrocnemius muscle and the relative elasticity of the Achilles tendon during a 1-hour treadmill run in healthy patients and in patients with AT. Study Design: Controlled laboratory study. Methods: Real-time harmonic contrast-enhanced ultrasound (CEU) measurements of the MV of the Achilles tendon were taken in 18 volunteers (9 patients with AT, 9 healthy controls). The CEU analyses were conducted before exercise, immediately after a 1-hour treadmill run, and 24 hours after exercise. Surface electromyography (EMG) signals of the gastrocnemius were recorded continuously during the 1-hour treadmill run. Results: In both the controls and the patients with AT, the MV of the Achilles tendon was increased after exercise as compared with before exercise ( P < .005). Additionally, the MV signal was significantly larger in the patients with AT before, immediately after, and 24 hours after the running exercise compared with values in healthy controls ( P < .0001). The muscle activation pattern differed in patients with AT compared with controls in that controls had increased EMG amplitudes at the end of the 1-hour treadmill run in the medial and lateral gastrocnemius ( P < .0001). Moreover, patients with AT had approximately 15% less elastic Achilles tendons at the beginning of the 1-hour treadmill run, a condition that did not change because of exercise. Conclusion: Acute exercise increases the MV of the Achilles tendon in healthy patients and patients with AT in a similar manner. However, patients with AT have a significantly larger MV at all time points compared with healthy patients, supporting the hypothesis that microvascular changes may be involved in the pathogenesis of tendinopathy. Clinical Relevance: This study underlines that tendon flexibility is altered in patients with AT and that CEU is a promising tool to establish the early diagnosis of this condition.
The effect of false positive breast screening examinations on subsequent attendance: retrospective cohort study
Authors: Maxwell Anthony J , Beattie Cathryn , Lavelle Janet , Lyburn Iain , Sinnatamby Ruchi , Garnett Shelagh , Herbert Annie 
Published: August 2013

Objectives To investigate the effect of false positive breast screening examination results on subsequent attendance in the UK National Health Service Breast Screening Programme. Methods 253,017 previously screened women who were invited for rescreening were studied. Attendance rates of women who had received a normal result at the last (index) screen were compared with those of women who had received a false positive result. The effects of age, type of index screening examination (prevalent or incident) and tissue sampling at assessment were investigated. Results Women who had a false positive prevalent index screening examination were significantly more likely to reattend than those who had a normal prevalent index screening examination (87.7% vs. 86.0%). There was no significant difference in reattendance rates between women who had a false positive incident index screening examination and those with a normal incident index screening examination. However, women who underwent needle sampling or open biopsy following false positive incident index screening examinations were 12% and 60% less likely to reattend, respectively, than women whose index screening examinations were normal ( p < 0.001), although there was variation between centres. Increasing age significantly reduced the likelihood of reattendance. The overall reattendance of women who had been screened only once was six percentage points lower than that of women who had been screened more than once. Conclusions The findings suggest that most women who undergo the breast screening assessment process retain confidence in breast screening. Needle sampling and open biopsy should be used judiciously in the assessment of screen-detected abnormalities in view of the reduced reattendance that results from their use after incident screening examinations.
Legislatures, Courts, and Statutory Control of the Bureaucracy across the U.S. States
Authors: McGrath Robert J. 
Published: August 2013

AbstractHow do state legislatures use statutory language to control policy implementation by state agencies? In this article, I consider—in a specific policy area and time period—the extent to which this decision is affected by legislative anticipation of the likely actions of state courts. Previous literature has argued that the legislative use of statutory language to control bureaucrats varies with the availability of nonstatutory methods of control, but it does not explicitly consider the potential role of courts. My expectations are derived from a simple formal model of executive–legislative relations and are supported when I test them using data on the number of words added to a state's Medicaid laws from 1995 to 1996. In particular, I find that state legislatures write longer, more constraining, statutes when the likelihood that state courts intervene on their behalf is neither very high nor very low.
Epidemiology of Hip Injuries in the National Basketball Association
Authors: Jackson Timothy J. , Starkey Chad , McElhiney Danielle , Domb Benjamin G. 
Published: August 2013

Background: Professional athletes are subject to various injuries that are often dictated by the nature of their sport. Professional basketball players previously have been shown to sustain injuries throughout the musculoskeletal system, most commonly to the ankle and knee. Purpose: The purpose of this study was to report the epidemiology of injuries specific to the pelvis, hip, and thigh and their effect on games missed in professional basketball players. Study Design: Descriptive epidemiological. Methods: Records were recalled from the National Basketball Association epidemiological database for athletic-related pelvis, hip, or thigh injuries that occurred from the 1988-1989 through the 2011-2012 seasons. The primary information collected included anatomic location where the injury occurred, when in the course of the season injury occurred, specific pathology, date, activity at the time of injury, injury mechanism, number of practices and games missed, and whether surgery was required. The number of practices and games missed were summed to yield the number of days missed per episode. Results: There were 2852 cases (14.6% of all athletic-related injuries) involving 967 individual players. In 1746 (61.2%) cases, injuries occurred during game competition. Across the course of this study, clinical incidence of injury to the pelvis, hip, or thigh was 1.50 per 100 players. The mean (±standard deviation) number of days missed per case was 6.3 ± 10.2. The quadriceps group was the most commonly injured structure (contusions and strains) and had a significantly higher game-related injury rate than other structures (0.96 per 100 athletic exposures, 95% confidence interval [CI] = 0.87-1.04). Players had the greatest risk (relative risk = 1.38, 95% CI = 1.26-1.52) of sustaining a strain than any other type of injury, with a game-related injury rate of 1.79 (95% CI = 1.67-1.90). The hamstring muscle group was the most frequently strained. Strains were more likely to occur in the preseason. Conclusion: Pelvis, hip, and thigh injuries in professional basketball players are commonly extra-articular strains and contusions and represent a significant burden of injury. The actual amount of intra-articular hip disorders may be underestimated, as the awareness of these disorders has increased only in the past decade. With the awareness of the types of injuries that occur in this region, focused injury prevention strategies may be beneficial to players and teams at all levels of competition, not only professional athletes.
PKIS: computational identification of protein kinases for experimentally discovered protein phosphorylation sites
Authors: Zou Liang , Wang Mang , Shen Yi , Liao Jie , Li Ao , Wang Minghui 
Published: August 2013

AbstractBackgroundDynamic protein phosphorylation is an essential regulatory mechanism in various organisms. In this capacity, it is involved in a multitude of signal transduction pathways. Kinase-specific phosphorylation data lay the foundation for reconstruction of signal transduction networks. For this reason, precise annotation of phosphorylated proteins is the first step toward simulating cell signaling pathways. However, the vast majority of kinase-specific phosphorylation data remain undiscovered and existing experimental methods and computational phosphorylation site (P-site) prediction tools have various limitations with respect to addressing this problem.ResultsTo address this issue, a novel protein kinase identification web server, PKIS, is here presented for the identification of the protein kinases responsible for experimentally verified P-sites at high specificity, which incorporates the composition of monomer spectrum (CMS) encoding strategy and support vector machines (SVMs). Compared to widely used P-site prediction tools including KinasePhos 2.0, Musite, and GPS2.1, PKIS largely outperformed these tools in identifying protein kinases associated with known P-sites. In addition, PKIS was used on all the P-sites in Phospho.ELM that currently lack kinase information. It successfully identified 14 potential SYK substrates with 36 known P-sites. Further literature search showed that 5 of them were indeed phosphorylated by SYK. Finally, an enrichment analysis was performed and 6 significant SYK-related signal pathways were identified.ConclusionsIn general, PKIS can identify protein kinases for experimental phosphorylation sites efficiently. It is a valuable bioinformatics tool suitable for the study of protein phosphorylation. The PKIS web server is freely available athttp://bioinformatics.ustc.edu.cn/pkis.
Changes in sub-cellular localisation of trophoblast and inner cell mass specific transcription factors during bovine preimplantation development
Authors: Madeja Zofia E , Sosnowski Jaroslaw , Hryniewicz Kamila , Warzych Ewelina , Pawlak Piotr , Rozwadowska Natalia , Plusa Berenika , Lechniak Dorota 
Published: August 2013

Abstract
Background
Preimplantation bovine development is emerging as an attractive experimental model, yet little is known about the mechanisms underlying trophoblast (TE)/inner cell mass (ICM) segregation in cattle. To gain an insight into these processes we have studied protein and mRNA distribution during the crucial stages of bovine development. Protein distribution of lineage specific markers OCT4, NANOG, CDX2 were analysed in 5-cell, 8–16 cell, morula and blastocyst stage embryos. ICM/TE mRNA levels were compared in hatched blastocysts and included: OCT4, NANOG, FN-1, KLF4, c-MYC, REX1, CDX2, KRT-18 and GATA6.

Results
At the mRNA level the observed distribution patterns agree with the mouse model. CDX2 and OCT4 proteins were first detected in 5-cell stage embryos. NANOG appeared at the morula stage and was located in the cytoplasm forming characteristic rings around the nuclei. Changes in sub-cellular localisation of OCT4, NANOG and CDX2 were noted from the 8–16 cell onwards. CDX2 initially co-localised with OCT4, but at the blastocyst stage a clear lineage segregation could be observed. Interestingly, we have observed in a small proportion of embryos (2%) that CDX2 immunolabelling overlapped with mitotic chromosomes.

Conclusions
Cell fate specification in cattle become evident earlier than presently anticipated – around the time of bovine embryonic genome activation. There is an intriguing possibility that for proper lineage determination certain transcription factors (such as CDX2) may need to occupy specific regions of chromatin prior to its activation in the interphase nucleus. Our observation suggests a possible role of CDX2 in the process of epigenetic regulation of embryonic cell fate.