bluuebunny/crossref_metadata_2025_split huggingface dataset:
I-STOP/PMP: A tool for monitoring prescription drug abuse in patients with chronic pain syndromes
Authors: Bahlani Sonia , Mehta Sandeep , King Alexandra , Moldwin Robert 
Published: March 2015

Background: Patients with chronic pain syndromes often require analgesics and muscle relaxants for symptom management. Unfortunately, many of these agents have abuse potential, and specific concerns have been raised with regard to drug-seeking behaviours that involve multiple clinicians dispensing similar, if not identical, agents. The Internet System for Tracking Over-Prescribing (I-STOP) is a newly implemented law targeted at monitoring the abuse and diversion of controlled substances in New York State. The purpose of this study is to describe the use and the potential for abuse of agents commonly used in the treatment of a variety of urological pelvic pain patients. Materials and methods: Clinical data from 397 patients with interstitial cystitis/bladder pain syndrome (IC/BPS), chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS), and pelvic floor dysfunction (PFD) were identified through medical record review over a consecutive 6-month period. These names were entered into the Prescription Monitoring Program (PMP) database to which all prescribers have access. I-STOP monitors prescription use over a prior 6-month interval from the date of access. Categoric data were compared with Fisher’s exact test and the chi-square test. Kaplan–Meier product limit curves were stratified by group and compared using the log-rank test. Results: In total, 397 patients with IC/BPS, CP/CPPS, and PFD were included; 14 patients (3.5%) had been prescribed duplicate medications by more than one practitioner. Of those patients who received duplicate prescriptions, 4 of 14 (29%) were for benzodiazepines, and 10 of 14 (71%) of duplicate medications prescribed were opioids. Conclusions: The implementation of the I-STOP/PMP registry provides an innovative way for practitioners to monitor prescribed controlled substances while allowing patients to receive necessary medications for symptom control.
Making sense of hematopoietic stem cell niches
Authors: Boulais Philip E. , Frenette Paul S. 
Published: March 2015

Abstract
The hematopoietic stem cell (HSC) niche commonly refers to the pairing of hematopoietic and mesenchymal cell populations that regulate HSC self-renewal, differentiation, and proliferation. Anatomic localization of the niche is a dynamic unit from the developmental stage that allows proliferating HSCs to expand before they reach the bone marrow where they adopt a quiescent phenotype that protects their integrity and functions. Recent studies have sought to clarify the complexity behind the HSC niche by assessing the contributions of specific cell populations to HSC maintenance. In particular, perivascular microenvironments in the bone marrow confer distinct vascular niches that regulate HSC quiescence and the supply of lineage-committed progenitors. Here, we review recent data on the cellular constituents and molecular mechanisms involved in the communication between HSCs and putative niches.
Doubling survival and improving clinical outcomes using a left ventricular assist device instead of chest compressions for resuscitation after prolonged cardiac arrest: a large animal study
Authors: Derwall Matthias , Brücken Anne , Bleilevens Christian , Ebeling Andreas , Föhr Philipp , Rossaint Rolf , Kern Karl B , Nix Christoph , Fries Michael 
Published: March 2015

Abstract
Introduction
Despite improvements in pre-hospital and post-arrest critical care, sudden cardiac arrest (CA) remains one of the leading causes of death. Improving circulation during cardiopulmonary resuscitation (CPR) may improve survival rates and long-term clinical outcomes after CA.

Methods
In a porcine model, we compared standard CPR (sCPR; n =10) with CPR using an intravascular cardiac assist device without additional chest compressions (iCPR; n =10) following 10 minutes of electrically induced ventricular fibrillation (VF). In a separate crossover experiment, 10 additional pigs were subjected to 10 minutes of VF and 6 minutes of sCPR; the iCPR device was then implanted if a return of spontaneous circulation (ROSC) was not achieved using sCPR. Animals were evaluated in respect to intra- and post-arrest hemodynamics, survival, functional outcome and cerebral and myocardial lesions following CPR. We hypothesized that iCPR would result in more frequent ROSC and better functional recovery than sCPR.

Results
iCPR produced a mean flow of 1.36 ± 0.02 L/min, leading to significantly higher coronary perfusion pressure (CPP) values during the early period of CPR (22 ± 10 mmHg vs. 9 ± 5 mmHg, P ≤0.01, 1 minute after start of CPR; 20 ± 11 mmHg vs. 10 ± 7 mmHg, P =0.03, 2 minutes after start of CPR), resulting in high ROSC rates (100% in iCPR vs. 50% in sCPR animals; P =0.03). iCPR animals showed significantly lower serum S100 levels at 10 and 30 minutes following ROSC (3.5 ± 0.6 ng/ml vs. 7.4 ± 3.0 ng/ml 30 minutes after ROSC; P ≤0.01), as well as superior clinical outcomes based on overall performance categories (2.9 ± 1.0 vs. 4.6 ± 0.8 on day 1; P ≤0.01). In crossover experiments, 80% of animals required treatment with iCPR after failed sCPR. Notably, ROSC was still achieved in six of the remaining eight animals (75%) after a total of 22.8 ± 5.1 minutes of ischemia.

Conclusions
In a model of prolonged cardiac arrest, the use of iCPR instead of sCPR improved CPP and doubled ROSC rates, translating into improved clinical outcomes.
Lobular breast cancer: incidence and genetic and non-genetic risk factors
Authors: Dossus Laure , Benusiglio Patrick R 
Published: March 2015

AbstractWhile most invasive breast cancers consist of carcinomas of the ductal type, about 10% are invasive lobular carcinomas. Invasive lobular and ductal carcinomas differ with respect to risk factors. Invasive lobular carcinoma is more strongly associated with exposure to female hormones, and therefore its incidence is more subject to variation. This is illustrated by US figures during the 1987 to 2004 period: after 12 years of increases, breast cancer incidence declined steadily from 1999 to 2004, reflecting among other causes the decreasing use of menopausal hormone therapy, and these variations were stronger for invasive lobular than for invasive ductal carcinoma. Similarly, invasive lobular carcinoma is more strongly associated with early menarche, late menopause and late age at first birth. As for genetic risk factors, four high-penetrance genes are tested in clinical practice when genetic susceptibility to breast cancer is suspected, BRCA1, BRCA2, TP53 and CDH1. Germline mutations in BRCA1 and TP53 are predominantly associated with invasive ductal carcinoma, while BRCA2 mutations are associated with both ductal and lobular cancers. CDH1, the gene coding for the E-cadherin adhesion protein, is of special interest as mutations are associated with invasive lobular carcinoma, but never with ductal carcinoma. It was initially known as the main susceptibility gene for gastric cancer of the diffuse type, but the excess of breast cancers of the lobular type in CDH1 families led researchers to identify it also as a susceptibility gene for invasive lobular carcinoma. The risk of invasive lobular carcinoma is high in female mutation carriers, as about 50% are expected to develop the disease. Carriers must therefore undergo intensive breast cancer screening, with, for example, yearly magnetic resonance imaging and mammogram starting at age 30 years.
CD27-IgD- memory B cells are modulated by in vivo interleukin-6 receptor (IL-6R) blockade in rheumatoid arthritis
Authors: Mahmood Zafar , Muhammad Khalid , Schmalzing Marc , Roll Petra , Dörner Thomas , Tony Hans-Peter 
Published: March 2015

Abstract
Introduction
Enhanced B cell activity, particularly memory B cells have gained interest in evaluating response during therapies with biologics. CD27-IgD- double-negative (DN) B cells lacking the conventional memory marker CD27 are reported to be part of the memory compartment, however, only scarce data is available for rheumatoid arthritis (RA). We therefore focused on DN B cells in RA, studied their isotypes and modulation during interleukin-6 receptor (IL-6R) inhibition by tocilizumab (TCZ).

Methods
DN B cells were phenotypically analyzed from 40 RA patients during TCZ at baseline week 12, week 24 and 1 year. A single B cell polymerase chain reaction (PCR) approach was used to study Ig receptors, VH gene rearrangements and specific isotypes.

Results
Phenotypic analysis showed a significantly expanded population of DN B cells in RA which contain a heterogeneous mixture of IgG-, IgA- and IgM-expressing cells with a clear dominance of IgG+ cells. DN B cells carry rearranged heavy chain gene sequences with a diversified mutational pattern consistent with memory B cells. In contrast to tumor necrosis factor alpha (TNF-α) inhibition, a significant reduction in mutational frequency of BCR gene rearrangements at week 12, 24 and 1 year (P <0.0001) was observed by in vivo IL-6R inhibition. These changes were observed for all BCR isotypes IgG, IgA and IgM at week 12, 24 and 1 year (P <0.0001). IgA-RF, IgA serum level and IgA+ DN B cells decreased significantly (P <0.05) at week 12 and week 24 during TCZ. Patients with a good European League Against Rheumatism (EULAR) response to TCZ had less DN B cells at baseline as compared to moderate responders (P = 0.006). Univariate logistic regression analysis revealed that the frequency of DN B cells at baseline is inversely correlated to a subsequent good EULAR response (P = 0.024) with an odds ratio of 1.48 (95% confidence interval as 1.05 to 2.06).

Conclusions
In RA, the heterogeneous DN B cell compartment is expanded and dominated by IgG isotype. TCZ can modulate the mutational status of DN Ig isotype receptors over 1 year. Interestingly, the frequency of DN B cells in RA may serve as a baseline predictor of subsequent EULAR response to TCZ.
Role of whole bone marrow, whole bone marrow cultured cells, and mesenchymal stem cells in chronic wound healing
Authors: Rodriguez-Menocal Luis , Shareef Shahjahan , Salgado Marcela , Shabbir Arsalan , Van Badiavas Evangelos 
Published: March 2015

Abstract
Introduction
Recent evidence has shown that bone marrow cells play critical roles during the inflammatory, proliferative and remodeling phases of cutaneous wound healing. Among the bone marrow cells delivered to wounds are stem cells, which can differentiate into multiple tissue-forming cell lineages to effect, healing. Gaining insight into which lineages are most important in accelerating wound healing would be quite valuable in designing therapeutic approaches for difficult to heal wounds.

Methods
In this report we compared the effect of different bone marrow preparations on established in vitro wound healing assays. The preparations examined were whole bone marrow (WBM), whole bone marrow (long term initiating/hematopoietic based) cultured cells (BMC), and bone marrow derived mesenchymal stem cells (BM-MSC). We also applied these bone marrow preparations in two murine models of radiation induced delayed wound healing to determine which had a greater effect on healing.

Results
Angiogenesis assays demonstrated that tube formation was stimulated by both WBM and BMC, with WBM having the greatest effect. Scratch wound assays showed higher fibroblast migration at 24, 48, and 72 hours in presence of WBM as compared to BM-MSC. WBM also appeared to stimulate a greater healing response than BMC and BM-MSC in a radiation induced delayed wound healing animal model.

Conclusions
These studies promise to help elucidate the role of stem cells during repair of chronic wounds and reveal which cells present in bone marrow might contribute most to the wound healing process.
The flavonoid profile of pigeonpea, Cajanus cajan: a review
Authors: Nix Aaron , Paull Cate A , Colgrave Michelle 
Published: March 2015

Abstract

Background
Pigeonpea is ranked as the sixth largest grain legume produced by volume and as such is a major global food crop for livestock and human consumption. We show that pigeonpea contains a number of flavonoids and report their distribution and concentration within different parts of the plant.

Findings
There are a total of 27 flavonoids reported in the literature representing seven flavonoid classes. We found no published evidence of flavanols (catechins/flavan-3-ols) or aurones reported from pigeonpea, nor any study of the flavonoids from pigeonpea flowers.

Conclusions
Despite over 40 years of research in to various aspects of pigeonpea we identified research gaps related to the phytochemical properties of pigeonpea. We explain how addressing these gaps could help to realise the full potential of pigeonpea in agricultural production.
Biogas production experimental research using algae
Authors: Baltrėnas Pranas , Misevičius Antonas 
Published: March 2015

Abstract
The current study is on the the use of macro-algae as feedstock for biogas production. Three types of macro-algae, Cladophora glomerata (CG), Chara fragilis (CF), and Spirogyra neglecta (SN), were chosen for this research. The experimental studies on biogas production were carried out with these algae in a batch bioreactor. In the bioreactor was maintained 35 ± 1°C temperature. The results showed that the most appropriate macro-algae for biogas production are Spirogyra neglecta (SN) and Cladophora glomerata (CG). The average amount of biogas obtained from the processing of SN – 0.23 m3/m3d, CG – 0.20 m3/m3d, and CF – 0.12 m3/m3d. Considering the concentration of methane obtained during the processing of SN and CG, which after eight days and until the end of the experiment exceeded 60%, it can be claimed that biogas produced using these algae is valuable. When processing CF, the concentration of methane reached the level of 50% only by the final day of the experiment, which indicates that this alga is less suitable for biogas production.
Estimation of slip rate and fault displacement during shallow earthquake rupture in the Nankai subduction zone
Authors: Hamada Yohei , Sakaguchi Arito , Tanikawa Wataru , Yamaguchi Asuka , Kameda Jun , Kimura Gaku 
Published: March 2015

Abstract
Enormous earthquakes repeatedly occur in subduction zones, and the slips along megathrusts, in particular those propagating to the toe of the forearc wedge, generate ruinous tsunamis. Quantitative evaluation of slip parameters (i.e., slip velocity, rise time and slip distance) of past slip events at shallow, tsunamigenic part of the fault is critical to characterize such earthquakes. Here, we attempt to quantify these parameters of slips that may have occurred along the shallow megasplay fault and the plate boundary décollement in the Nankai Trough, off southwest Japan. We apply a kinetic modeling to vitrinite reflectance profiles on the two fault rock samples obtained from Integrated Ocean Drilling Program (IODP). This approach constitutes two calculation procedures: heat generation and numerical profile fitting of vitrinite reflectance data. For the purpose of obtaining optimal slip parameters, residue calculation is implemented to estimate fitting accuracy. As the result, the measured distribution of vitrinite reflectance is reasonably fitted with heat generation rate

$$ \left(\dot{Q}\right) $$

Q
˙

and slip duration (t

r
) of 16,600 J/s/m2 and 6,250 s, respectively, for the megasplay and 23,200 J/s/m2 and 2,350 s, respectively, for the frontal décollement, implying slow and long-term slips. The estimated slip parameters are then compared with previous reports. The maximum temperature, Tmax, for the Nankai megasplay fault is consistent with the temperature constraint suggested by a previous work. Slow slip velocity, long-term rise time, and large displacement are recognized in these fault zones (both of the megasplay, the frontal décollement). These parameters are longer and slower than typical coseismic slip, but are rather consistent with rapid afterslip.
Percutaneous Native Renal Biopsy Adequacy: A Successful Interdepartmental Quality Improvement Activity
Authors: Geldenhuys Laurette , Nicholson Peter , Sinha Namita , Dini Angela , Doucette Steve , Alfaadhel Talal , Keough Valerie , West Michael 
Published: March 2015

Background: An adequate renal biopsy is essential for diagnosis and treatment of medical renal disease. Objective: We evaluated two initiatives to improve adequacy of renal biopsy samples at our centre. Design: Retrospective determination of renal biopsy adequacy. Setting: Queen Elizabeth II Health Sciences Centre. Patients: Patients undergoing medical renal biopsies. Measurements: Renal biopsy adequacy. Methods: The first initiative was to restrict the performance of biopsies to a smaller group of radiologists and to include a comment on biopsy adequacy in every pathology report. The second initiative was to introduce on-site adequacy assessment by a medical laboratory technologist. Native renal and allograft biopsy adequacies were calculated for three periods: 1) baseline, October 2005 to September 2006; 2) after implementation of the first initiative, January 2007 to September 2011; and 3) after implementation of the second initiative, October 2011 to September 2012. A subset of native renal biopsies was examined to determine if there was a relationship between adequacy and number of passes. Results: The percentages of adequate native renal biopsies during the first, second, and third periods were 31%, 72% and 90%, respectively. This represents a significant increase (40%, p < 0.0001) in adequacy following the first initiative, and another significant increase (18%, p = 0.0003) following the second initiative. The percentages of adequate renal allograft biopsies during the first, second, and third periods were 75%, 56% and 69%, respectively. These changes in adequacy were not statistically significant. In the subset of native renal biopsies examined, a biopsy comprising more than three cores was not associated with increase in adequacy. Limitations: The most important limitation is the lack of generally accepted and applied adequacy criteria limiting generalizability of our findings. Conclusions: Restricting the performance of biopsies to subspecialist operators, including an adequacy statement in the renal biopsy report and on-site adequacy assessment were effective in significantly improving native renal biopsy adequacy. This improvement appeared unrelated to an increase in the number of passes taken with a biopsy needle. Neither initiative improved the low adequacy of allograft biopsies.
Experimental validation of fracture aperture determination from borehole electric microresistivity measurements
Authors: Ponziani Michel , Slob Evert , Luthi Stefan , Bloemenkamp Richard , Le Nir Isabelle 
Published: March 2015

We studied the electric response of fractures with laboratory experiments and numerical simulations for a full-bore formation microimaging tool. The laboratory setup was designed and built to perform controlled experiments with accurate measurements of all principal properties involved for electric borehole imaging. These properties are formation resistivity, mud resistivity, fracture aperture, pad position, and button current. The experiments were conducted on two types of limestone for fracture apertures ranging from 0.1 to 0.9 mm and mud/formation resistivity contrasts varying from 1/100 to 1/10,000. A numerical model was used to reproduce the laboratory configuration and to validate the results. The model proved to be an effective tool to optimize the experimental setup, and it was also used to study the effect of standoff (up to 5 mm) on the measured integrated additional current. Linear relationships between the fracture aperture and measured integrated current were found to be valid for the laboratory experiment and the corresponding numerical simulation. The measured integrated current could therefore be used to determine the fracture aperture if the other parameters are known. Two coefficients in the relationship were found to differ from those previously found using numerical simulations for the actual borehole situation. These differences are attributed to tool- and scale-dependent factors.
Fetal Exposure to Sertraline Hydrochloride Impairs Pancreatic β-Cell Development
Authors: De Long Nicole E. , Gutgesell Marie K. , Petrik James J. , Holloway Alison C. 
Published: March 2015

AbstractTen percent to 15% of women take selective serotonin reuptake inhibitor (SSRI) antidepressants during pregnancy. Offspring exposed to SSRIs are more likely to have low birth weight; this is associated with an increased risk of development of diabetes in adulthood in part due to altered pancreatic development. The effects of perinatal exposure to SSRIs on pancreatic development are unknown. Therefore, the objective of this study was to determine the effect of fetal exposure to sertraline hydrochloride on pregnancy outcomes and pancreatic development. Wistar rats were given vehicle (n = 5) or sertraline hydrochloride (10 mg/kg/d; n = 8) via daily subcutaneous injection from the confirmation of mating until parturition. Results from this animal model demonstrated that offspring born to sertraline-exposed dams have no changes in birth weight but had a reduction in pancreatic β-cell area. The altered pancreatic islet development was a result of altered gene expression regulating islet development and survival. Therefore, fetal exposure to sertraline reduces β-cell capacity at birth, raising concerns regarding the long-term metabolic sequelae of such exposures.
Adamtsl2 deletion results in bronchial fibrillin microfibril accumulation and bronchial epithelial dysplasia – a novel mouse model providing insights into geleophysic dysplasia
Authors: Hubmacher Dirk , Wang Lauren W. , Mecham Robert P. , Reinhardt Dieter P. , Apte Suneel S. 
Published: March 2015

ABSTRACT
Mutations in the secreted glycoprotein ADAMTSL2 cause recessive geleophysic dysplasia (GD) in humans and Musladin–Lueke syndrome (MLS) in dogs. GD is a severe, often lethal, condition presenting with short stature, brachydactyly, stiff skin, joint contractures, tracheal-bronchial stenosis and cardiac valve anomalies, whereas MLS is non-lethal and characterized by short stature and severe skin fibrosis. Although most mutations in fibrillin-1 (FBN1) cause Marfan syndrome (MFS), a microfibril disorder leading to transforming growth factor-β (TGFβ) dysregulation, domain-specific FBN1 mutations result in dominant GD. ADAMTSL2 has been previously shown to bind FBN1 and latent TGFβ-binding protein-1 (LTBP1). Here, we investigated mice with targeted Adamtsl2 inactivation as a new model for GD (Adamtsl2−/− mice). An intragenic lacZ reporter in these mice showed that ADAMTSL2 was produced exclusively by bronchial smooth muscle cells during embryonic lung development. Adamtsl2−/− mice, which died at birth, had severe bronchial epithelial dysplasia with abnormal glycogen-rich inclusions in bronchial epithelium resembling the cellular anomalies described previously in GD. An increase in microfibrils in the bronchial wall was associated with increased FBN2 and microfibril-associated glycoprotein-1 (MAGP1) staining, whereas LTBP1 staining was increased in bronchial epithelium. ADAMTSL2 was shown to bind directly to FBN2 with an affinity comparable to FBN1. The observed extracellular matrix (ECM) alterations were associated with increased bronchial epithelial TGFβ signaling at 17.5 days of gestation; however, treatment with TGFβ-neutralizing antibody did not correct the epithelial dysplasia. These investigations reveal a new function of ADAMTSL2 in modulating microfibril formation, and a previously unsuspected association with FBN2. Our studies suggest that the bronchial epithelial dysplasia accompanying microfibril dysregulation in Adamtsl2−/− mice cannot be reversed by TGFβ neutralization, and thus might be mediated by other mechanisms.