bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of Helicobacter pylori‐positive and Helicobacter pylori‐negative gastric mucosa‐associated lymphoid tissue lymphoma and their influence on clinical outcome
Authors: Choi Yoon Jin , Kim Nayoung , Paik Jin Ho , Kim Jung Mogg , Lee Sang Hyub , Park Young Soo , Hwang Jin‐Hyeok , Kim Jin‐Wook , Jeong Sook‐Hyang , Lee Dong Ho , Jung Hyun Chae 
Published: January 2013

AbstractBackgroundTo compare clinicopathologic and molecular characteristics of low‐grade gastric mucosa‐associated lymphoid tissue lymphoma depending on Helicobacter pylori positivity and to find out a predictive factor for unresponsiveness to Helicobacter pylori eradication therapy in Korea.MethodsA total of 53 Helicobacter pylori‐positive and 13 negative mucosa‐associated lymphoid tissue lymphoma patients were enrolled, and tissues from 21 patients were investigated to examine the presence of t(11;18)(q21;q21) with fluorescence in situ hybridization. Clinicopathologic features such as the endoscopic appearance, dominant site of lesion, depth of invasion, clinical stage, and the existence of MALT1 gene rearrangement were compared between these two groups. Fifty‐six patients who underwent H. pylori eradication therapy were divided into responder and nonresponder groups. The two groups were analyzed to calculate odds ratios for resistance to the eradication.ResultsHelicobacter pylori‐negative gastric mucosa‐associated lymphoid tissue lymphoma patients averaged a more advanced clinical stage than H. pylori‐positive (p = .023) patients. The frequency of t(11;18)/API2‐MALT1 did not differ between H. pylori‐positive (45.5%) and H. pylori‐negative cases (55.6%). Thirty‐eight of 51 (74.5%) H. pylori‐positive patients achieved complete regression after the eradication, while 2 of 5 (40%) H. pylori‐negative patients obtained regression. Presence of lesions in both distal and proximal parts of stomach (p = .041) and bearing of t(11;18)(q21;q21) (p = .007) were predictors for nonresponsiveness for H. pylori eradication.ConclusionsHelicobacter pylori eradication could be performed as a primary therapy regardless of H. pylori status, and assessing t(11;18)/API2‐MALT1 would be considered after failure to remission by H. pylori eradication.
Medieval Domestic Devotion
Authors: Deane Jennifer Kolpacoff 
Published: January 2013

AbstractFor generations, scholars of history, religion, anthropology, theology and other disciplines have pored over texts, artifacts, and ritual behaviors, seeking to understand better the nature of medieval religious experience. Yet until recently, the intriguing intersections of home and the holy during the Middle Ages received little sustained attention – a circumstance due to issues of both limited sources and historiographical emphasis. Informed by interdisciplinary contributions from gender studies, anthropology, spatial studies, and other fields, however, recent research has cast new light on how medieval women and men constructed and enacted devotional practice in domestic spaces. This article introduces readers to both the historical and historiographical patterns shaping current understandings of domestic devotion during the Middle Ages. After a brief survey of key scholarly shifts in the late twentieth century that set the stage for new approaches, focus turns to ways in which medieval people encountered the sacred at home: these include prayer, reading (devotional books and books of hours), and the use of material objects in spiritual practice such as relics, rosaries, and portable altars. The final section suggests several possible directions for future research.
An improved mouse model that rapidly develops fibrosis in non‐alcoholic steatohepatitis
Authors: Matsumoto Masahiko , Hada Natsuko , Sakamaki Yoshiyuki , Uno Akiko , Shiga Toshihiko , Tanaka Chiaki , Ito Tsuneo , Katsume Asao , Sudoh Masayuki 
Published: January 2013

SummaryNon‐alcoholic steatohepatitis (NASH) is a progressive fibrotic disease, the pathogenesis of which has not been fully elucidated. One of the most common models used in NASH research is a nutritional model where NASH is induced by feeding a diet deficient in both methionine and choline. However, the dietary methionine‐/choline‐deficient model in mice can cause severe weight loss and liver atrophy, which are not characteristics of NASH seen in human patients. Exclusive, long‐term feeding with a high‐fat diet (HFD) produced fatty liver and obesity in mice, but the HFD for several months did not affect fibrosis. We aimed to establish a mouse model of NASH with fibrosis by optimizing the methionine content in the HFD. Male mice were fed a choline‐deficient, L‐amino acid‐defined, high‐fat diet (CDAHFD) consisting of 60 kcal% fat and 0.1% methionine by weight. After 1–14 weeks of being fed CDAHFD, the mice were killed. C57BL/6J mice maintained or gained weight when fed CDAHFD, while A/J mice showed a steady decline in body weight (of up to 20% of initial weight). In both strains of mice, plasma levels of alanine aminotransferase increased from week 1, when hepatic steatosis was also observed. By week 6, C57BL/6J mice had developed enlarged fatty liver with fibrosis as assessed by Masson's trichrome staining and by hydroxyproline assay. Therefore, this improved CDAHFD model may be a mouse model of rapidly progressive liver fibrosis and be potentially useful for better understanding human NASH disease and in the development of efficient therapies for this condition.
Improvement of erectile function in patients with benign prostatic hyperplasia undergoing transurethral plasmakinetic resection of the prostate
Authors: Zhang Hai‐Min , Zheng Jun‐Hua , Xu Yun‐Fei , Peng Bo , Yan Yang , Gao Qi‐Ruo 
Published: January 2013

ObjectiveAlthough transurethral resection of the prostate remains the gold standard surgical treatment for benign prostatic hyperplasia, transurethral plasmakinetic resection of the prostate has become a popular alternative. This study investigated the effects of plasmakinetic resection of the prostate on erectile function.MethodsA total of 400 patients that underwent plasmakinetic resection of the prostate or transurethral resection of the prostate were prospectively enrolled in this study. Of these, 384 patients met the inclusion criteria. One experienced surgeon carried out all the procedures. The International Prostate Symptom Score, International Index of Erectile Function‐5, maximum flow rate and ultrasound postvoid residual volume were determined, and evaluated preoperatively and at 12 months postoperatively. Prostate‐specific antigen, age and prostate volume of each patient were recorded.ResultsThe median International Index of Erectile Function‐5 score of plasmakinetic resection of the prostate patients significantly increased from 8.0 (interquartile range 7.0–9.0) preoperatively to 21.0 (19.0–22.0) at 12 months postoperatively (P < 0.05). The score of plasmakinetic resection of the prostate patients was significantly higher than that of the transurethral resection of the prostate group (P < 0.05); however, the International Prostate Symptom Score of the plasmakinetic resection of the prostate group was not significantly different from that of the transurethral resection of the prostate group (P > 0.05).ConclusionsA significant improvement in erectile function can be observed at 12 months in patients undergoing plasmakinetic resection of the prostate. Despite these encouraging findings, the effects of plasma kinetic resection of the prostate on erectile function remain to be further studied.
Radical prostatectomy in high‐risk prostate cancer
Authors: Ischia Joseph , Gleave Martin 
Published: January 2013

AbstractOne consistent finding in the studies regarding treating men with prostate cancer is that men with high‐risk disease have the most to gain from treatment with curative intent. Men with high‐risk or locally‐advanced prostate cancer require treatment to the primary cancer or risk dying prematurely from their disease. Increasingly, combined androgen deprivation therapy + radiation treatment is seen as the standard treatment as a result of prospective studies in this space, and the perceived increased morbidity of radical prostatectomy in the setting of a “low” cure rate as monotherapy. In the absence of a well‐conducted randomized trial, there is no definite evidence that one treatment is superior to the other. The advantages of radical prostatectomy are that it provides excellent local control of the primary tumor without an increase in morbidity, accurately stages the disease to guide further therapy, and removes benign sources of prostate‐specific antigen so that failures can be promptly identified and subsequent treatment can be initiated in a timely manner. Although several guidelines recommend radiation treatment over radical prostatectomy as first‐line treatment, there is no evidence that surgery is inferior and radical prostatectomy should remain part of any informed discussion regarding treatment options for men with high‐risk prostate cancer.
Post‐chemotherapy nerve‐sparing laparoscopic retroperitoneal lymph node dissection in stage IIB testicular cancer
Authors: Kimura Yasunori , Nakamura Terukazu , Kawauchi Akihiro , Kawabata Gaku , Hongo Fumiya , Miki Tsuneharu 
Published: January 2013

AbstractWe report our experience of extraperitoneal nerve‐sparing laparoscopic retroperitoneal lymph node dissection after chemotherapy. Six patients were diagnosed with non‐seminomatous germ cell tumor after orchiectomy and clinical stage IIB disease. Nerve‐sparing laparoscopic retroperitoneal lymph node dissection was carried out for residual retroperitoneal tumors after cisplatin‐based chemotherapy. The median tumor diameter was 2.95 cm before chemotherapy and 1.95 cm after chemotherapy. A modified left (n = 1), right (n = 1) and bilateral (n = 4) template for the dissection area was used. Surgery was successfully completed in all patients and no conversion to open surgery was necessary. Median operative time was 394 min (range 212–526 min). Median blood loss was 75 mL (range 10–238 mL). The overall complication rate was 33.3% (2/6). Two patients had prolonged lymphatic leakage (grade I), which was managed conservatively. Antegrade ejaculation was preserved in all six patients. The histopathological findings showed that two patients had mature teratoma and four patients had necrotic tissue. After a median follow up of 30 months (range 24–36), no recurrence of disease was observed. We can conclude that extraperitoneal nerve‐sparing laparoscopic retroperitoneal lymph node dissection for residual tumors after chemotherapy is a feasible operation. The oncological outcomes need to be confirmed in a certain number of patients with longer follow up.
Interventions for women exposed to acute intimate partner violence: emergency professionals’ perspective
Authors: Leppäkoski Tuija , Paavilainen Eija 
Published: January 2013

Aims and objectives. To examine interventions and practices carried out by the emergency department professionals.Background. Developing the related practices provides an opportunity for early intervention in women exposed to intimate partner violence who visit the emergency department.Design. The descriptive, cross‐sectional multi‐centre designed study with a convenience sample was used to acquire more information to illustrate the situation of intervening in intimate partner violence at Finnish emergency departments.Participants. A total of 24‐hour emergency departments from all over Finland participated in the study.Methods. Research materials were collected by questionnaires given to emergency department professionals (n = 950) and 51% were returned.Data analysis. The data were analysed using quantitative methods. Chi‐square test was used to test the statistical significance of the data. A p‐value of <0·05 was considered to indicate statistical significance of each analysis.Results. The findings of this study reveal that the emergency department professionals who had common practices and written procedures for handling intimate partner violence reported having helped both women and perpetrators more often than those without. Good cooperation with different help providers, opportunities to consult them and training received facilitated intervention methods.Conclusions. There is a need for more effective change of information and cooperation with different help providers concerning how to act with women and their family members in intimate partner violence situations. Emergency department professionals do not know enough about the legislation concerning intimate partner violence and their professional duty, e.g. if a child’s health and well‐being are in danger.Relevance to clinical practice. This information can be utilised in research, professional education, further training and practical work and when planning the prevention of intimate partner violence.
External Carotid Dissection and External Carotid Proatlantal Intersegmental Artery with Subclavian Steal Prompting External Carotid and Subclavian Artery Stenting
Authors: Abla Adib A. , Kan Peter , Jahshan Shady , Dumont Travis M. , Levy Elad I. , Siddiqui Adnan H. 
Published: January 2013

ABSTRACTBACKGROUND AND PURPOSEThe authors describe a case of a proatlantal intersegmental artery seen in the setting of external carotid artery dissection and subclavian steal due to proximal subclavian artery stenosis.METHODSAn 83‐year‐old woman presented with left homonymous hemianopsia and a right posterior cerebral artery distribution stroke. She was found to have severe left subclavian artery stenosis proximal to the vertebral artery (VA) and an occluded contralateral VA. Doppler ultrasonography and angiography both showed a dissection of the proximal left external carotid artery. Left common carotid artery angiography also demonstrated a connection between the external carotid and vertebral arteries at the C1 level with anterograde flow into the vertebrobasilar system and retrograde flow toward the vertebral origin, consistent with subclavian steal.RESULTSThe patient underwent successful stenting of the subclavian and external carotid arteries with resolution of anterograde flow in the left VA.CONCLUSIONSThis case represents an interesting presentation of both subclavian steal and an external carotid artery to VA anastomosis. Also, the presence of a dissection of the external carotid artery represents a rare finding.
A role for two‐pore potassium (K2P) channels in endometrial epithelial function
Authors: Patel Suraj K. , Jackson Leigh , Warren Averil Y. , Arya Pratibha , Shaw Robert W. , Khan Raheela N. 
Published: January 2013

AbstractThe human endometrial epithelium is pivotal to menstrual cycle progression, implantation and early pregnancy. Endometrial function is directly regulated by local factors that include pH, oxygen tension and ion concentrations to generate an environment conducive to fertilization. A superfamily of potassium channels characterized by two‐pore domains (K2P) and encoded by KCNK genes is implicated in the control of the cell resting membrane potential through the generation of leak currents and modulation by various physicochemical stimuli. The aims of the study were to determine the expression and function of K2P channel subtypes in proliferative and secretory phase endometrium obtained from normo‐ovulatory women and in an endometrial cancer cell line. Using immunochemical methods, real‐time qRT‐PCR proliferation assays and electrophysiology. Our results demonstrate mRNA for several K2P channel subtypes in human endometrium with molecular expression of TREK‐1 shown to be higher in proliferative than secretory phase endometrium (P < 0.001). The K2P channel blockers methanandamide, lidocaine, zinc and curcumin had antiproliferative effects (P < 0.01) in an endometrial epithelial cancer cell line indicating a role for TASK and TREK‐1 channels in proliferation. Tetraethylammonium‐ and 4‐aminopyridine‐insensitive outwards currents were inhibited at all voltages by reducing extracellular pH from 7.4 to 6.6. Higher expression of TREK‐1 expression in proliferative phase endometrium may, in part, underlie linked to increased cell division. The effects of pH and a lack of effect of non‐specific channel blockers of voltage‐gated potassium channels imply a role for K2P channels in the regulation of human endometrial function.
Epigenetic regulation of Thy‐1 gene expression by histone modification is involved in lipopolysaccharide‐induced lung fibroblast proliferation
Authors: He Zhengyu , Wang Xiangrui , Deng Yuxiao , Li Wen , Chen Yongming , Xing Shunpeng , Zhao Xianyuan , Ding Jia , Gao Yuan 
Published: January 2013

AbstractLipopolysaccharide (LPS)‐induced pulmonary fibrosis is characterized by aberrant proliferation and activation of lung fibroblasts. Epigenetic regulation of thymocyte differentiation antigen 1 (Thy‐1) is associated with lung fibroblast phenotype transformation that results in aberrant cell proliferation. However, it is not clear whether the epigenetic regulation of Thy‐1 expression is required for LPS‐induced lung fibroblast proliferation. To address this issue and better understand the relative underlying mechanisms, we used mouse lung fibroblasts as model to observe the changes of Thy‐1 expression and histone deacetylation after LPS challenge. The results showed that cellular DNA synthesis, measured by BrdU incorporation, was impacted less in the early stage (24 hrs) after the challenge of LPS, but significantly increased at 48 or 72 hrs after the challenge of LPS. Meanwhile, Thy‐1 expression, which was detected by real‐time PCR and Western blot, in lung fibroblasts decreased with increased time after LPS challenge and diminished at 72 hrs. We also found that the acetylation of either histone H3 or H4 decreased in the LPS‐challenged lung fibroblasts. ChIP assay revealed that the acetylation of histone H4 (Ace‐H4) decreased in the Thy‐1 promoter region in response to LPS. In addition, all the above changes could be attenuated by depletion of TLR4 gene. Our studies indicate that epigenetic regulation of Thy‐1 gene expression by histone modification is involved in LPS‐induced lung fibroblast proliferation.
Pharmacologically active microcarriers influence VEGF‐A effects on mesenchymal stem cell survival
Authors: Penna Claudia , Perrelli Maria‐Giulia , Karam Jean‐Pierre , Angotti Carmelina , Muscari Claudio , Montero‐Menei Claudia N. , Pagliaro Pasquale 
Published: January 2013

AbstractResistance of transplanted mesenchymal stem cells (MSCs) in post‐ischemic heart is limited by their poor vitality. Vascular‐endothelial‐growth‐factor‐A (VEGF‐A) as such or slowly released by fibronectin‐coated pharmacologically‐active‐microcarriers (FN‐PAM‐VEGF) could differently affect survival kinases and anti‐apoptotic mediator (e.g. Bcl‐2). Therefore VEGF‐A or FN‐PAM‐VEGF could differently enhance cell proliferation, and/or resistance to hypoxia/reoxygenation (H/R) of MSCs. To test these hypotheses MSCs were incubated for 6‐days with VEGF‐A alone or with FN‐PAM‐VEGF. In addition, MSCs pre‐treated for 24‐hrs with VEGF‐A or FN‐PAM‐VEGF were subsequently exposed to H/R (72‐hrs 3% O2 and 3‐hrs of reoxygenation). Cell‐proliferation and post‐hypoxic vitality were determined. Kinases were studied at 30‐min., 1‐ and 3‐days of treatment. Cell‐proliferation increased about twofold (P < 0.01) 6‐days after VEGF‐A treatment, but by a lesser extent (55% increase) with FN‐PAM‐VEGF (P < 0.05). While MSC pre‐treatment with VEGF‐A confirmed cell‐proliferation, pre‐treatment with FN‐PAM‐VEGF protected MSCs against H/R. In the early phase of treatments, VEGF‐A increased phospho‐Akt, phospho‐ERK‐1/2 and phospho‐PKCε compared to the untreated cells or FN‐PAM‐VEGF. Afterword, kinase phosphorylations were higher with VGEF, except for ERK‐1/2, which was similarly increased by both treatments at 3 days. Only FN‐PAM‐VEGF significantly increased Bcl‐2 levels. After H/R, lactate dehydrogenase release and cleaved Caspase‐3 levels were mainly reduced by FN‐PAM‐VEGF. While VEGF‐A enhances MSC proliferation in normoxia, FN‐PAM‐VEGF mainly hampers post‐hypoxic MSC death. These different effects underscore the necessity of approaches suited to the various conditions. The use of FN‐PAM‐VEGF could be considered as a novel approach for enhancing MSC survival and regeneration in hostile environment of post‐ischemic tissues.
Pregnancy outcome following bariatric surgery
Authors: Khan Rahat , Dawlatly Bashir , Chappatte Oliver 
Published: January 2013

Key content

There is an exponential increase in the number of morbidly obese women of childbearing age undergoing bariatric surgery as the ultimate treatment for their obesity.

Pregnancy after bariatric surgery is safer, with fewer complications, than pregnancy in morbidly obese women.

Patients should be strongly advised not to get pregnant for at least 12–18 months following bariatric surgery.

Multidisciplinary care before, during and after pregnancy following bariatric surgery helps to prevent nutrition‐related and surgical complications.

Learning objectives

To outline the safety, advantages and limitations of bariatric surgery procedures in relation to maternal and neonatal outcomes.

To understand that the key to a healthy pregnancy after weight‐loss surgery is paying attention to nutrition.

To learn the importance of the multidisciplinary management of post‐surgery pregnancies, including prepregnancy counselling.

Ethical issues

Often GPs and obstetricians are not fully informed about the management of pregnant individuals who have had bariatric surgery.

There are no recommendations that detail the nutrients that women should consume during pregnancy if they have had previous weight loss surgery.

A literature review demonstrates both positive and negative associations of weight loss procedures with fertility.
Management of postpartum hypertension
Authors: Smith Marie , Waugh Jason , Nelson‐Piercy Catherine 
Published: January 2013

Key content

Appropriate treatment of postnatal hypertension is essential to prevent maternal morbidity and mortality from cerebral haemorrhage.

Women with pre‐eclampsia remain at risk of serious complications following delivery and should continue to be monitored as an inpatient for at least 72 hours.

Compared with the antenatal period, a wider choice of antihypertensive agents are available to prescribe for the postnatal patient. An understanding of the basic pharmacology and risk–benefit profiles of each agent will facilitate patient‐centred prescribing.

Following discharge, the community team should be given clear guidelines for ongoing management of hypertension. A hospital postnatal appointment should be offered to discuss future pregnancy and cardiovascular risk.

Learning objectives

Review postpartum cardiovascular physiology following normal and hypertensive pregnancies.

Demonstrate the principles of management and a suggested approach to management of postpartum hypertension based on NICE guidance.

Discuss antihypertensive agents prescribed for women in the postpartum period.