bluuebunny/crossref_metadata_2025_split huggingface dataset:
IL-33–Mediated Innate Response and Adaptive Immune Cells Contribute to Maximum Responses of Protease Allergen–Induced Allergic Airway Inflammation
Authors: Kamijo Seiji , Takeda Haruna , Tokura Tomoko , Suzuki Mayu , Inui Kyoko , Hara Mutsuko , Matsuda Hironori , Matsuda Akira , Oboki Keisuke , Ohno Tatsukuni , Saito Hirohisa , Nakae Susumu , Sudo Katsuko , Suto Hajime , Ichikawa Saori , Ogawa Hideoki , Okumura Ko , Takai Toshiro 
Published: April 2013

Abstract
How the innate and adaptive immune systems cooperate in the natural history of allergic diseases has been largely unknown. Plant-derived allergen, papain, and mite allergens, Der f 1 and Der p 1, belong to the same family of cysteine proteases. We examined the role of protease allergens in the induction of Ab production and airway inflammation after repeated intranasal administration without adjuvants and that in basophil/mast cell stimulation in vitro. Papain induced papain-specific IgE/IgG1 and lung eosinophilia. Der f 1 induced Der f 1–specific IgG1 and eosinophilia. Although papain-, Der f 1–, and Der p 1–stimulated basophils expressed allergy-inducing cytokines, including IL-4 in vitro, basophil-depleting Ab and mast cell deficiency did not suppress the papain-induced in vivo responses. Protease inhibitor–treated allergens and a catalytic site mutant did not induce the responses. These results indicate that protease activity is essential to Ab production and eosinophilia in vivo and basophil activation in vitro. IL-33–deficient mice lacked eosinophilia and had reduced papain-specific IgE/IgG1. Coadministration of OVA with papain induced OVA-specific IgE/IgG1, which was reduced in IL-33–deficient mice. We demonstrated IL-33 release, subsequent IL-33–dependent IL-5/IL-13 release, and activation of T1/ST2-expressing lineage−CD25+CD44+ innate lymphoid cells in the lung after papain inhalation, suggesting the contribution of the IL-33–type 2 innate lymphoid cell–IL-5/IL-13 axis to the papain-induced airway eosinophilia. Rag2-deficient mice, which lack adaptive immune cells, showed significant, but less severe, eosinophilia. Collectively, these results suggest cooperation of adaptive immune cells and IL-33–responsive innate cells in protease-dependent allergic airway inflammation.
TAK1–JNK Axis Mediates Survival Signal through Mcl1 Stabilization in Activated T Cells
Authors: Hirata Yasuko , Sugie Ayano , Matsuda Akio , Matsuda Satoshi , Koyasu Shigeo 
Published: April 2013

Abstract
TAK1, a member of MAPK kinase kinase (MAPKK-K) family, can activate JNK, p38 MAPK, and NF-κB signaling pathways. Although targeted gene disruption studies have demonstrated that TAK1 plays a critical role in T cell functions, precise functions of downstream mediators remain elusive. We used the chemical compound LL-Z1640-2, which preferentially suppressed MAPK activation but not NF-κB signal downstream of TAK1. LL-Z1640-2 blocked TCR-induced T cell proliferation and activation, confirming that a TAK1-mediated MAPK signal is essential for T cell activation. LL-Z1640-2 induced apoptosis of activated mouse splenic T cells in a caspase- and caspase-activated DNase–dependent manner. TAK1-JNK pathway, which is activated downstream of IL-2R, induced the phosphorylation of antiapoptotic protein Mcl1 in activated T cells, resulting in the stabilization of Mcl1 protein. Our data uncover that among signal transduction pathways downstream of TAK1, JNK mediates a survival program through Mcl1 stabilization downstream of IL-2R in activated T cells and that blockade of TAK1-JNK pathway can eliminate activated T cells by apoptosis.
Microwave Monolithic Power Amplifier Design
Authors: Campbell Charles 
Published: April 2013

AbstractThis article describes some key elements of high‐frequency microwave monolithic integrated circuit (MMIC) power amplifier design. An example specification for a 23‐GHz, two‐stage saturated power amplifier is provided and described in detail illustrating how the requirements impact the overall design. This is followed by an analysis of measured transistor data to determine an appropriate device cell for the amplifier. The detailed design of the MMIC is described in the next section including circuit synthesis, simulation, layout of the microstrip matching networks, and the impact of external components. The article is concluded with a section on how to perform a stability analysis of the final MMIC power amplifier.
Synthesis of biopolyols by mild oxypropylation of liquefied starch and its application to polyurethane rigid foams
Authors: Yoshioka Mariko , Nishio Yoshiyuki , Saito Daisuke , Ohashi Hironori , Hashimoto Masayuki , Shiraishi Nobuo 
Published: April 2013

AbstractThe liquefaction of starch in the presence of commercial polyol resulted in liquefaction products (LPs) with much lower hydroxyl values compared to those resulting from glycerol liquefaction. With the use of the obtained LPs (as initiators) and a catalytic amount of potassium hydroxide, oxypropylation was conducted under mild conditions (at 120°C, inner pressure generation of <0.2 MPa). Urethane resin foams were prepared from the obtained biopolyols. The reactivities of the biopolyols toward isocyanates were almost the same as those of commercial polyols. The physical properties of the obtained foams were as good or better than the ones obtained with the commercial polyols, including the sucrose/glycerol polyol GR‐84T. The biomass content values of the foams prepared with the biopolyols ranked among the highest in the world. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Retraction: Synthesis, Characterization, and Biological–Pharmacological Evaluation of New Phosphazenes Bearing Dioxybiphenyl and Schiff Base Groups
Authors: Cil Erol , Tanyildizi Mehmet Ali , Ozen Furkan , Boybay Mustafa , Arslan Mustafa , Gorgulu Ahmet Orhan 
Published: April 2013

AbstractRetraction StatementThe following article from the Archiv der Pharmazie,“Synthesis, Characterization, and Biological–Pharmacological Evaluation of New Phosphazenes Bearing Dioxybiphenyl and Schiff Base Groups” by Erol Cil, Mehmet Ali Tanyildizi, Furkan Ozen, Mustafa Boybay, Mustafa Arslan and Ahmet Orhan Gorgulu, published online on 13 March 2012 in Wiley Online Library (wileyonlinelibrary.com), has been retracted by agreement between the authors, the journal Editor‐in‐Chief Prof. Holger Stark and Wiley‐VCH Verlag GmbH & Co. KGaA Weinheim. The retraction has been agreed due to errors in the biological‐pharmacological results data and in the discussion and conclusions based on these data.
Molecular Assembly of an Achiral Phosphine and a Chiral Primary Amine: A Highly Efficient Supramolecular Catalyst for the EnantioselectiveMichael Reaction of Aldehydes with Maleimides
Authors: Yang Wei , Jiang Ke‐Zhi , Lu Xing , Yang Hua‐Meng , Li Li , Lu Yixin , Xu Li‐Wen 
Published: April 2013

AbstractA combined catalyst system of a cinchonidine‐derived primary amine and triphenylphosphine (CD‐NH2/PPh3) exhibited high catalytic performance in the Michael reaction of aldehydes with maleimides, thereby affording the corresponding functionalized aldehydes in excellent yields (up to 99 %) and enantioselectivities (>99 % ee). More interestingly, the significance of the phosphine in enhancing the enantioselectivities in the chiral‐primary‐amine‐catalyzed Michael reaction was revealed. Furthermore, we explored the origin of the reaction mechanism in the Michael addition promoted by the dual organocatalytic system. On the basis of experimental results and spectroscopic analysis, such as UV/Vis, fluorescence emission (FL), NMR, and circular dichroism (CD) spectroscopy, as well as ESI‐MS, we found that the molecular assembly of phosphine and primary amine played a crucial role in this enantioselective reaction, in which a possible supramolecular complex was formed as an effective chiral catalyst through noncovalent molecular interactions of a cinchona alkaloid‐derived primary amine with triphenylphosphine.
Verfahren zur Berechnung von Stützwänden
Authors: Franke D. , Neuberg C. , Winkler A. 
Published: April 2013

AbstractEs werden ein Berechnungsalgorithmus und Programm zur Berechnung von Stützwänden vorgestellt, welches eine bessere Berücksichtigung der Bodeneigenschaften als herkömmliche Bettungszahlverfahren erlaubt. Es kann sowohl für den stützenden als auch für den belastenden Erddruck eine nichtlinear elastisch‐plastische Mobilisierungsfunktion definiert werden, um damit den unmittelbaren Zusammenhang von Erddruck und Verschiebung zu erfassen. Es entfallen die in vielen Verfahren verwendeten linear wirkenden Federauflager zur Simulierung des Erdwiderstandes. Als Ergebnis der Berechnungen erhält man die Verformungen der Baugrubenwand, den mobilisierten Erddruck, die Summe aller vertikalen Kräfte und Schnittkräfte sowie den Ausnutzungsgrad der passiven Erddruckkraft.
Modellierung von diskontinuierlich verbundenen Holz‐Beton‐Verbunddecken ‐ Teil 2: Langzeittragverhalten
Authors: Grosse M. , Hartnack R. , Rautenstrauch K. 
Published: April 2013

AbstractIm Teil 1 dieses Beitrages wurde gezeigt, daß bei diskontinulerlich verbundenen Holz‐Beton‐Verbunddecken das in der DIN 1052 dargestellte Gamma‐Verfahren nur sehr begrenzt für die Abschätzung des Tragverhaltens geeignet ist. Für die praxisgerechte Berechnung und Dimensionierung solcher Verbunddeckenelemente kann daher das im Teil 1 vorgestellte Stabwerksmodell verwendet werden. Ergänzend hierzu werden im Teil 2 die Ergebnisse der Langzeitsimulation des Tragverhaltens auf Basis dieses Stabwerkmodells vorgestellt. In dem verwendeten Simulationsprogramm können sowohl das klimaabhängige Kriech‐ und Schwindverhalten der beiden Baustoffe Holz und Beton als auch gegebenenfalls auftretende Nichtlinearitäten und Temperatureinflüsse realitätsnah berücksichtigt werden. Aufbauend auf diese Simulationen werden dem Tragwerksplaner Hinweise zur Abschätzung des Langzeittragverhaltens von Holz‐Beton‐Verbundkonstruktionen mittels üblicher Stabwerksprogramme gegeben.
Faltung von textilen Membranen bei wandelbaren Leichtbaukonstruktionen
Authors: Göppert Knut , Paech Christoph , Balz Markus 
Published: April 2013

AbstractUnlängst wurden zwei der weltweit größten wandelbaren Dächer fertig gestellt. Die faltbaren Dächer des Nationalstadions Warschau und BC Place in Vancouver sind beide nahezu 10000 m2 groß. Während das Stadion in Warschau mit einer mechanisch vorgespannten Membran realisiert wurde, erhielt das Stadion in Vancouver aufgrund der hohen Schneelasten pneumatisch stabilisierte Kissen. Im folgenden Bericht wird speziell auf die erforderlichen Materialeigenschaften für wandelbare Membrankonstruktionen eingegangen, da ein immer wiederkehrender Faltungsvorgang eine signifikante Beanspruchung für das Material und seine Beschichtung bedeutet. Anhand von neu entwickelten Testmethoden wird beschrieben, wie in Abhängigkeit des Entwurfes die passenden Materialien gewählt wurden.Folding of textile membranes in retractable lightweight structuresTwo of the largest retractable membrane roofs in the world were recently completed. The National Stadium in Warsaw and BC Place Stadium in Vancouver have retractable membrane portions each with sizes up to 10000 m2. The Stadium in Warsaw has a mechanically pre‐stressed membrane while the structure in Vancouver uses inflated membrane cushions to withstand severe snow loading. In the following article required material properties for retractable membrane solutions will be discussed, since the repetitive folding of the membrane during the retraction puts significant demands to the textile material and its coating system. By means of especially developed testing methods it is described how the most suitable membrane material can be found, taken into account the concept design of the project.
Developmental profiles of the murine palatal methylome
Authors: Seelan Ratnam S. , Appana Savitri N. , Mukhopadhyay Partha , Warner Dennis R. , Brock Guy N. , Pisano M. Michele , Greene Robert M. 
Published: April 2013

BACKGROUNDEnvironmental factors contribute to the etiology of cleft palate (CP). Identification of genes that are methylated during development of the secondary palate will contribute to a better understanding of the gene‐environment link contributing to CP.METHODSGenomic DNA fragments from secondary palate tissue from gestational days (GDs) 12 to 14 were subjected to Selective Enrichment of Methylated DNA (SEMD) and used to probe NimbleGen 2.1M mouse promoter arrays. Input (control) and SEMD samples were labeled with Cy3 and Cy5, respectively, and used for array hybridization (three arrays per GD). Data were analyzed using the Bioconductor packageRingo. Gene methylation was verified by pyrosequencing analysis and expression by quantitative real‐time PCR.RESULTSA total of 5577 methylated genes were identified during palate development: (1) 74% of genes were methylated on all three GDs; (2) CpG islands accounted for only 30% of methylated regions of interest (MRIs); (3) location of MRIs was more often observed in gene bodies (73%) than in promoters; (4) evaluation of MRIs on GDs 12–14 revealed no significant differentially methylated regions; (5) DAVID analysis of MRIs revealed that the cadherin and Wnt signaling pathways, as well as pathways involved in proteoglycan synthesis, were significantly enriched for methylated genes.CONCLUSIONSOur prior studies identified differentially expressed mRNAs and microRNAs in the developing palate. The current study complements these studies by identifying genes whose expression may be altered as a result of DNA methylation.Birth Defects Research (Part A) 97:171–186, 2013. © 2013 Wiley Periodicals, Inc.
Innovationen im Brückenbau
Authors: Friedrich Heinz , Empelmann Martin , Hamm Stephan , Freundt Ursula , Schnellenbach‐Held Martina , Butz Christiane , Mangerig Ingbert , Tusche Jens 
Published: April 2013

AbstractDie Abteilung Straßenbau des Bundesministeriums für Verkehr, Bau und Stadtentwicklung (BMVBS) hat in Zusammenarbeit mit der Bundesanstalt für Straßenwesen (BASt) ein langfristiges Rahmenforschungsprogramm unter dem Titel “Straße im 21. Jahrhundert” für ihre gemeinsamen Forschungsaktivitäten erstellt. Um unsere Straßen zukunftsfähig zu gestalten, werden ganzheitliche Lösungsansätze verfolgt, bei denen u. a. auch der Brücken‐ und Ingenieurbau maßgeblich involviert ist. Im nachfolgenden Beitrag wird ein Überblick über drei abgeschlossene und zwei laufende Forschungsprojekte zum Thema Innovationen im Brückenbau gegeben.Innovations in Bridge ConstructionThe Federal Ministry of Transport, Building and Urban Development (BMVBS) together with the Federal Highway Research Institute (BASt) created a long‐term framework research program called “Road in the 21st Century”. The design of future‐compliant roads requires an integral approach also including bridges and structural technology. The following article deals with three completed and two current research projects with the focus on Innovations in Bridge Construction.
Perivascular cells in blood vessel regeneration
Authors: Wanjare Maureen , Kusuma Sravanti , Gerecht Sharon 
Published: April 2013

AbstractVascular engineering seeks to design and construct functional blood vessels comprising endothelial cells (ECs) and perivascular cells (PCs), with the ultimate goal of clinical translation. While EC behavior has been extensively investigated, PCs play an equally significant role in the development of novel regenerative strategies, providing functionality and stability to vessels. The two major classes of PCs are vascular smooth muscle cells (vSMCs) and pericytes; vSMCs can be further sub‐classified as either contractile or synthetic. The inclusion of these cell types is crucial for successful regeneration of blood vessels. Furthermore, understanding distinctions between vSMCs and pericytes will enable improved therapeutics in a tissue‐specific manner. Here we focus on the approaches and challenges facing the use of PCs in vascular regeneration, including their characteristics, stem cell sources, and interactions with ECs. Finally, we discuss biochemical and microRNA (miR) regulators of PC behavior and engineering approaches that mimic various cues affecting PC function.
Impaired postoperative leucocyte counts after preoperative radiotherapy for rectal cancer in the Stockholm III Trial
Authors: Pettersson D , Glimelius B , Iversen H , Johansson H , Holm T , Martling A 
Published: April 2013

Abstract

Background
Radiotherapy (RT) in rectal cancer increases postoperative morbidity. A suggested reason is RT-induced bone marrow depression resulting in impaired leucocyte counts. The ongoing Stockholm III Trial randomizes patients with operable rectal cancers to short-course RT with immediate surgery (SRT), short-course RT with surgery delayed for 4–8 weeks (SRT-delay) and long-course RT with surgery delayed for 4–8 weeks (LRT-delay). This study examined differences between the randomization arms regarding leucocyte response and postoperative complications.

Methods
Patients randomized in the Stockholm III Trial between October 1998 and November 2010 were included. Data were collected in a prospective register. Additional data were obtained by retrospective review of clinical records.

Results
Of 657 randomized patients, 585 had data on leucocytes. The SRT arm had the highest proportion of postoperative complications (SRT, 52·5 per cent; SRT-delay, 39·4 per cent; LRT-delay, 41 per cent; P = 0·010). There was no association between low preoperative leucocyte count and postoperative complications (P = 0·238). Irrespective of randomization arm, patients with an impaired postoperative to preoperative leucocyte ratio had the highest rate of complications (low ratio, 56·6 per cent; intermediate ratio, 46·9 per cent; high ratio, 36·3 per cent; P = 0·010). The SRT arm had the highest proportion of low ratios (SRT, 48·9 per cent; SRT-delay, 22·8 per cent; LRT-delay, 22 per cent; P &lt; 0·001).

Conclusion
An impaired postoperative leucocyte response is associated with postoperative complications. The highest risk is with immediate surgery following short-course radiotherapy. Registration number: NCT 00904813 (http://www.clinicaltrials.gov).
Unsaturated NHC Complexes Immobilized by the Backbone: Synthesis and Application
Authors: Li Peng , Herrmann Wolfgang A. , Kühn Fritz E. 
Published: April 2013

AbstractThe synthesis and an exemplary catalytic application of a SBA‐15‐supported rhodium–N‐heterocyclic carbene (NHC) complex is reported. In contrast to the conventional method of immobilization of unsaturated NHC complexes, the rhodium–NHC compound described here is connected to the SBA‐15 surface by a linker originating from the backbone of the unsaturated NHC ligand. The unique characteristics of the new immobilization mode enable the supported NHC complexes to maintain two unchanged “wing‐tip” ligands. This method of immobilization of unsaturated NHC complexes provides a new way to maintain the original configuration of homogeneous NHC complexes in the heterogenized catalytic system. The immobilized rhodium–NHC catalyst is used in a hydrogenation reaction with styrene as substrate.
Fluoreszenzfarbstoffe in der Natur
Authors: Weiß Dieter , Brandl Herbert 
Published: April 2013

AbstractIm vorliegenden Beitrag werden einige in der Natur vorkommende, fluoreszierende Pflanzen‐ und Pilzinhaltsstoffe vorgestellt. Dieses Gebiet der Naturstoffchemie wurde und wird bis dato nur wenig bearbeitet. In vielen Originalarbeiten findet man zwar die einzelnen Stoffe beschrieben, aber nur in den seltensten Fällen gibt es Hinweise auf ein eventuelles Fluoreszenzvermögen. Ausgestattet mit einer UV‐Lampe, Reagenzgläsern und einigen ausgewählten Lösemitteln lassen sich Naturstoffe leicht auf eine eventuelle Fluoreszenz testen. Im Rahmen dieser Publikation wird von den Autoren die noch nicht bekannte Hypericin‐sensibilisierte Peroxyoxalat‐Chemolumineszenz beschrieben.
H2O – Jo mei!
Authors: Roth Klaus 
Published: April 2013

AbstractWasser ist ein komplexes Netzwerk von H2O‐Molekülen, die über schwache Wasserstoffbrückenbindungen verbunden sind. Alle anomalen physikalischen und chemischen Eigenschaften basieren auf diesen Wasserstoffbrückenbindungen und deren kurzer Lebensdauer in der Größenordnung von Pikosekunden. Obwohl Untersuchungen an Eis und Wasserdampf strukturelle Hinweise geben, ist flüssiges Wasser immer noch voller Geheimnisse. Das eröffnet besonders in Hinblick auf unser Trinkwasser Raum für wilde Spekulationen, abwegige Theorien und Hokuspokus. Die Fülle der vorgeschlagenen Verbesserungsmethoden sind Zeugnisse sowohl für die schier grenzenlose Fantasie und wissenschaftliche Kreativität, als auch für die Scharlatanerie, kriminelle Energie und blinde Vertrauensseligkeit der Menschen.
Fast Determination of Phenanthrene in Soil by Gas Chromatography‐Mass Spectrometry Using Chemometric Resolution and Standard Addition Method
Authors: Yu Weiwei , Cai Wensheng , Shao Xueguang 
Published: April 2013

AbstractA method for fast determination of the component in complex samples by using gas chromatography‐mass spectrometry (GC‐MS) was developed and used for quantitative analysis of phenanthrene in soils. In the method, window independent component analysis (WICA) was used for resolving the mass spectrum and non‐negative immune algorithm (NNIA) was employed for obtaining the chromatographic profile. Therefore, spectral and chromatographic information of a specific component can be obtained from the measured GC‐MS data of overlapping and high background. Six soil samples collected from different places were analyzed. The tedious pretreatments in preparing the samples and the elution in the separation were simplified for speeding up the analysis. Due to the complexity of the matrix, standard addition method was adopted for the final quantification. The applicability of the method was validated with a spiked sample and the results of the six samples are reasonable.
Selective Chemosensor of Fe3+ Based on Fluorescence Quenching by 2,2′‐Bisbenzimidazole Derivative in Aqueous Media
Authors: Wei Taibao , Liu Jun , Yao Hong , Lin Qi , Xie Yongqiang , Shi Bingbing , Zhang Peng , You Xingmei , Zhang Youming 
Published: April 2013

Abstract2,2′‐Bisbenzimidazole derivative (L) was designed as a fluorescent chemosensor for Fe3+. This structurally simple chemosensor displays significant fluorescence quenching with increasing concentrations of Fe3+. L exhibited high selectivity and antidisturbance for Fe3+ among environmentally relevant metal ions in aqueous media. The method of Job's plot indicated the formation of 1:2 complex between L and Fe3+, and the possible binding mode of the system was also proposed. In addition, further study demonstrates the detection limit on fluorescence response of the sensor to Fe3+ is down to 10−7 mol·L−1 range. The binding mode was investigated by fluorescence spectra, ESI‐MS, IR data, 1H NMR, 13C NMR and crystal data.
Fast Determination of Ethylendiamine in Aminophylline Tablets by Small‐sized Capillary Electrophoresis with Amperometric Detection
Authors: Ge Jinyuan , Tong Fanghong , Li Ying , Zhang Yang , Chu Qingcui , Ye Jiannong 
Published: April 2013

AbstractA novel method for fast determination of ethylendiamine (EDA) in Aminophylline Tablets has been developed by small‐sized capillary electrophoresis with amperometric detection (small‐CE‐AD) coupled with field‐amplified sample injection (FASI). Under the optimum conditions, EDA and four aliphatic diamine homologs (1,3‐diaminopropane, 1,4‐diaminobutane, 1,5‐diaminopentane and 1,6‐diaminohexane) could be well separated within 6 min at a separation voltage of 2.0 kV in an acetate buffer solution of pH 3.8 with low limit of detection (LOD) of 1.3×10−11 g/mL for EDA (S/N=3). The proposed method has been successfully applied to direct determination of EDA content in different batches of Aminophylline Tablets. The method does not require off‐line preconcentration and derivatization steps, which should find wide application fields including pharmaceuticals as an alternative to conventional and microchip CE approaches.