bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, Nilaparvata lugens (Stål) (Hemiptera: Delphacidae)
Authors: Wan P.-J. , Yang L. , Yuan S.-Y. , Tang Y.-H. , Fu Q. , Li G.-Q. 
Published: April 2015

AbstractThe brown planthopper Nilaparvata lugens is a serious phloem-feeding pest of rice in China. The current study focuses on a saccharopine dehydrogenase (SDH) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (Lys), which plays a role in insect growth and carnitine production (as a substrate). The protein, provisionally designated as NlylsSDH [a SDH derived from yeast-like symbiont (YLS) in N. lugens], had a higher transcript level in abdomens, compared with heads, wings, legs and thoraces, which agrees with YLS distribution in N. lugens. Ingestion of Nlylssdh targeted double-stranded RNA (dsNlylssdh) for 5, 10 and 15 days decreased the mRNA abundance in the hoppers by 47, 70 and 31%, respectively, comparing with those ingesting normal or dsegfp diets. Nlylssdh knockdown slightly decreased the body weights, significantly delayed the development of females, and killed approximately 30% of the nymphs. Moreover, some surviving adults showed two apparent phenotypic defects: wing deformation and nymphal cuticles remained on tips of the legs and abdomens. The brachypterours/macropterours and sex ratios (female/male) of the adults on the dsRNA diet were lowered compared with the adults on diets without dsRNA. These results suggest that Nlylssdh encodes a functional SDH protein. The adverse effect of Nlylssdh knockdown on N. lugens implies the importance of Lys in hopper development. This study provides a proof of concept example that Nlylssdh could serve as a possible dsRNA-based pesticide for planthopper control.
Asteroid (Echinodermata) skeletal elements from upper Oligocene deposits of Jamaica and Antigua
Authors: BLAKE DANIEL B. , DONOVAN STEPHEN K. , MAH CHRISTOPHER L. , DIXON HAROLD L. 
Published: April 2015

AbstractThe Antillean Cenozoic fossil record of asteroids comprises mainly dissociated ossicles. Most common among isolates from upper Oligocene deposits of Jamaica and Antigua are marginal ossicles of an extinct, indeterminate species ofPycinaster. This is the youngest known occurrence of the genus and the first from beyond Europe. A number of relatively complete fossils have been assigned toPycinasterand (sub)familial status proposed for it together withPhocidaster. The latter proposition is based solely on a few marginals, but available diagnoses are judged insufficient to justify such recognition. The taxon Pycinasteridae is here synonymized with the Goniasteridae, although future study of added features (such as the ventral surface) might justify recognition at a higher taxonomic level. In addition to ossicles assigned toPycinaster, many marginals are tentatively assigned to the surviving goniasteridNymphaster. Numerous generic and many species names have been based on asteroid isolates, but the practice demands assumptions that are not readily justified. Linkage of discrete isolates under a single taxon name assumes derivation from a single source, an inference that can be verified only rarely (if ever), therefore reducing names to the single holotype ossicle. Availability of only isolates encourages comparison with extant taxa and biogeography, biasing interpretations with a Holocene overlay. Because of these constraints, a new nominal species ofPycinasteris not justified and assignment of ossicles toNymphasteris tentative. However, given the importance of asteroids in marine communities, we emphasize the significance, largely ignored, of their presence in Cenozoic deposits of the wider Caribbean.
Laser-assisted proton collision on light nuclei at moderate energies
Authors: Barna I.F. , Varró S. 
Published: April 2015

AbstractWe present a non-relativistic analytic quantum mechanical model to calculate angular differential cross-sections for laser-assisted proton nucleon scattering on a Woods–Saxon optical potential where the nth-order photon absorption is taken into account simultaneously. With this novel description we can integrate two well-established fields, namely low-energy nuclear physics and multi-photon processes together. As a physical example we calculate cross-sections for proton–12C collision at 49 MeV in the laboratory frame in various realistic laser fields. We consider optical Ti:sapphire and X-ray lasers with intensities which are available in existing laser facilities or in the future ELI or X-FEL.
Structural priming in Spanish as evidence of implicit learning
Authors: GÁMEZ PERLA B. , SHIMPI PRIYA M. 
Published: April 2015

ABSTRACTThis study uses a structural priming technique with young Spanish speakers to test whether exposure to a rare syntactic form in Spanish (fue-passive) would increase the production and comprehension of that form. In Study 1, 14 six-year-old Spanish speakers described pictures of transitive scenes. This baseline study revealed thatfue-passives were virtually non-existent in children's spontaneous speech. Using the priming technique in Study 2, an additional 56 Spanish-speaking children were exposed tofue-passive or active picture descriptions; we varied whether children repeated the modeled form. With repetition, production offue-passives increased past baseline usage. When not asked to repeat, comprehension and production offue-passives was no different than chance. Results extend the existing literature by experimentally testing input effects on the production and comprehension of infrequently used constructions, further corroborating the relation between input frequency and language skill. Findings are consistent with the view that an implicit learning mechanism guides language learning.
Investigation of travel-related cases in a multinational outbreak: example of the Shiga-toxin producingE. colioutbreak in Germany, May–June 2011
Authors: DEVAUX I. , VARELA-SANTOS C. , PAYNE-HALLSTRÖM L. , TAKKINEN J. , BOGAARDT C. , COULOMBIER D. 
Published: April 2015

SUMMARYEarly investigation of travel-related cases in an outbreak of an emerging infectious disease can provide useful information to epidemiologists to characterize the exposure, while they may differ in demographic profiles from cases reported in the country where the outbreak has occurred. During the spring 2011E. colioutbreak in Germany, we proposed a methodological approach to collect a minimal set of demographic and clinical data that are relatively easy to obtain and available at an early stage of an outbreak investigation. Ninety-eight STEC O104 travel-related cases were reported in a survey by seven EU countries, Switzerland, Canada and the USA. We found a mean incubation period (n= 50) of 8·5 days, which confirmed previous estimations communicated by the Robert Koch Institute. No significant association was found between the duration of the incubation period and possible demographic and clinical factors, although the older the age, the shorter the incubation period that was observed. Such approach and observations are informative for further investigations of outbreaks of enterohaemorrhagicE. colior other emerging infectious diseases.
Critical Citizens: Attitudes towards Democracy in Indonesia and Malaysia
Authors: PIETSCH JULIET , CLARK MARSHALL 
Published: April 2015

AbstractIn recent years much has been said about how new democracies are backsliding or have regressed since the turn of the century when hope and optimism about the future spread of democracy was widespread. However, ideas that democracy would spread were based on institutional and governance indicators rather than from the perspective of everyday citizens. When we look at public attitudes towards democracy during this period, we can see that such optimism was perhaps misplaced or premature. Drawing on findings from the AsiaBarometer and the World Values Survey, this research finds that public attitudes during this time were not overly convinced by democracy and certainly not yet satisfied with their government's performance in terms of providing basic democratic freedoms and independence.
Reclaiming Singapore's ‘Growth with Equity’ Social Compact
Authors: RAHIM LILY ZUBAIDAH 
Published: April 2015

AbstractSingapore's long-serving People's Action Party (PAP) government suffered from a major electoral setback in the 2011 general election and subsequent by-elections. The high-growth population policy, underpinned by the influx of migrants and foreign workers, has strongly fuelled the groundswell of public discontent and is commonly perceived to have contributed to widening income disparities, wage stagnation, and cost of living pressures. This article attempts to make sense of the PAP leadership's dogged commitment to the high-growth population policy despite the electoral backlashes and policy criticisms by prominent public intellectuals and others closely connected to the PAP establishment. It considers Singapore's high-growth population policy and widening income disparity within the context of the authoritarian developmental state's shift away from the ‘growth with equity’ social compact. The article also examines the impact of widening income inequality and other policy lapses on the legitimacy of the PAP government as the clamor for a renewed social compact based on ‘growth with equity’ gathers momentum in the repoliticized polity.
Ethnic Politics and Consociationalism in the 2013 Malaysian Election
Authors: SEGAWA NORIYUKI 
Published: April 2015

AbstractThis paper examines the prospects of ethnic politics and consociationalism in Malaysia, in terms of their structures and capabilities. Towards this end, the Barisan Nasional's (BN) policies from 2008 to 2013, the BN's and the Pakatan Rakyat's (PR) 2013 electoral strategies, and the results of the 2013 election will be analysed. As political parties in both the BN and the PR have generally represented ethnic interests and identities, an ethnic-based political structure has been maintained. A consociational political system, which is based on an ethnic-based political structure, has been adopted by both political coalitions. An examination of the 2013 election results indicates that the BN's ethnic politicking as a vote-gathering device has become ineffective. The BN's consociationalism has suffered from a legitimacy deficit, particularly within ethnic minority communities. However, it may be premature to claim that the Malaysian political culture is on the cusp of transcending the BN's paradigm of ethnic politics and consociationalism. The shift from ethnic politics will generate stronger momentum when the PR parties are incorporated into one political party.
Indonesia's Democratic Performance: A Popular Assessment
Authors: MUJANI SAIFUL , LIDDLE R. WILLIAM 
Published: April 2015

AbstractHow democratic is contemporary Indonesia? While analysts differ, Indonesian citizens, when asked in systematic public opinion surveys conducted regularly by the authors since 1999, consistently express strong support for democratic principles and also believe that their country's democratic performance is high. Support for democratic performance is highly correlated with support for government performance, as measured by perceptions of the condition of the national economy and political system. At the same time, higher levels of education and income, in Indonesia as in other countries, have created a considerable number of critical citizens, that is, citizens who value democracy but are critical of its performance. On our evidence for Indonesia, it is members of this group who are the most motivated and best prepared to demand a higher level of democratic performance from their elected officials.
Qualitative and quantitative variation in the mechanisms of salinity tolerance determined by multivariate assessment of diverse rice (Oryza sativa L.) genotypes
Authors: Hossain Mohammad Rashed , Pritchard Jeremy , Ford-Lloyd Brian V. 
Published: April 2015

Climate change-induced events are causing salinization of many rice-growing areas, requiring the identification of new sources of genetic variation for salt tolerance in plant genetic resources since commonly grown cultivars are sensitive to salt. To identify the level of salt tolerance across a wide range of genotypes, we used a multivariate screening method using multiple growth and physiological traits simultaneously. For this purpose, four indica, two japonica and two wild rice genotypes were grown hydroponically under 40 and 80 mM NaCl stresses; fourteen different growth, qualitative and physiological traits, e.g. plant height, biomass, root and shoot elongation rates, and tissue ion accumulation, were recorded. In general, indica varieties performed better than both japonica and wild species. Our approach identified the existence of qualitatively different mechanisms of salt tolerance across the genotypes. For example, Pokkali, a salt-tolerant indica variety, displayed both ‘Na exclusion’ and ‘ion balance’ mechanisms, whereas PSBRc50 and IR58 showed only ‘Na exclusion’, and the Japonica genotypes Banikat and Nipponbare showed only ‘ion balance’. The results demonstrated that the tolerance is dependent on the level of stress and that this varies between genotypes; Nipponbare is moderately tolerant to 40 mM NaCl but not to 80 mM. We also suggest that the use of multivariate analyses can simplify the complex salinity tolerance picture and can effectively reveal the salinity tolerant genotype from a wide range of germplasm. The results reported here identify different physiological mechanism of tolerance across the genotypes and provide a sound basis for future studies examining their underlying molecular mechanisms.
Non-symbiotic Bradyrhizobium ecotypes dominate North American forest soils
Authors: VanInsberghe David , Maas Kendra R , Cardenas Erick , Strachan Cameron R , Hallam Steven J , Mohn William W 
Published: April 2015

Abstract
The genus Bradyrhizobium has served as a model system for studying host–microbe symbiotic interactions and nitrogen fixation due to its importance in agricultural productivity and global nitrogen cycling. In this study, we identify a bacterial group affiliated with this genus that dominates the microbial communities of coniferous forest soils from six distinct ecozones across North America. Representative isolates from this group were obtained and characterized. Using quantitative population genomics, we show that forest soil populations of Bradyrhizobium represent ecotypes incapable of nodulating legume root hairs or fixing atmospheric nitrogen. Instead, these populations appear to be free living and have a greater potential for metabolizing aromatic carbon sources than their close symbiotic relatives. In addition, we identify fine-scaled differentiation between populations inhabiting neighboring soil layers that illustrate how diversity within Bradyrhizobium is structured by habitat similarity. These findings reconcile incongruent observations about this widely studied and important group of bacteria and highlight the value of ecological context to interpretations of microbial diversity and taxonomy. These results further suggest that the influence of this genus likely extends well beyond facilitating agriculture, especially as forest ecosystems are large and integral components of the biosphere. In addition, this study demonstrates how focusing research on economically important microorganisms can bias our understanding of the natural world.
Forest harvesting reduces the soil metagenomic potential for biomass decomposition
Authors: Cardenas Erick , Kranabetter J M , Hope Graeme , Maas Kendra R , Hallam Steven , Mohn William W 
Published: April 2015

Abstract
Soil is the key resource that must be managed to ensure sustainable forest productivity. Soil microbial communities mediate numerous essential ecosystem functions, and recent studies show that forest harvesting alters soil community composition. From a long-term soil productivity study site in a temperate coniferous forest in British Columbia, 21 forest soil shotgun metagenomes were generated, totaling 187 Gb. A method to analyze unassembled metagenome reads from the complex community was optimized and validated. The subsequent metagenome analysis revealed that, 12 years after forest harvesting, there were 16% and 8% reductions in relative abundances of biomass decomposition genes in the organic and mineral soil layers, respectively. Organic and mineral soil layers differed markedly in genetic potential for biomass degradation, with the organic layer having greater potential and being more strongly affected by harvesting. Gene families were disproportionately affected, and we identified 41 gene families consistently affected by harvesting, including families involved in lignin, cellulose, hemicellulose and pectin degradation. The results strongly suggest that harvesting profoundly altered below-ground cycling of carbon and other nutrients at this site, with potentially important consequences for forest regeneration. Thus, it is important to determine whether these changes foreshadow long-term changes in forest productivity or resilience and whether these changes are broadly characteristic of harvested forests.
Mechanisms of splicing-dependent trans-synaptic adhesion by PTPδ–IL1RAPL1/IL-1RAcP for synaptic differentiation
Authors: Yamagata Atsushi , Yoshida Tomoyuki , Sato Yusuke , Goto-Ito Sakurako , Uemura Takeshi , Maeda Asami , Shiroshima Tomoko , Iwasawa-Okamoto Shiho , Mori Hisashi , Mishina Masayoshi , Fukai Shuya 
Published: April 2015

AbstractSynapse formation is triggered through trans-synaptic interaction between pairs of pre- and postsynaptic adhesion molecules, the specificity of which depends on splice inserts known as ‘splice-insert signaling codes’. Receptor protein tyrosine phosphatase δ (PTPδ) can bidirectionally induce pre- and postsynaptic differentiation of neurons by trans-synaptically binding to interleukin-1 receptor accessory protein (IL-1RAcP) and IL-1RAcP-like-1 (IL1RAPL1) in a splicing-dependent manner. Here, we report crystal structures of PTPδ in complex with IL1RAPL1 and IL-1RAcP. The first immunoglobulin-like (Ig) domain of IL1RAPL1 directly recognizes the first splice insert, which is critical for binding to IL1RAPL1. The second splice insert functions as an adjustable linker that positions the Ig2 and Ig3 domains of PTPδ for simultaneously interacting with the Ig1 domain of IL1RAPL1 or IL-1RAcP. We further identified the IL1RAPL1-specific interaction, which appears coupled to the first-splice-insert-mediated interaction. Our results thus reveal the decoding mechanism of splice-insert signaling codes for synaptic differentiation induced by trans-synaptic adhesion between PTPδ and IL1RAPL1/IL-1RAcP.
Sub-nanometre resolution imaging of polymer–fullerene photovoltaic blends using energy-filtered scanning electron microscopy
Authors: Masters Robert C. , Pearson Andrew J. , Glen Tom S. , Sasam Fabian-Cyril , Li Letian , Dapor Maurizio , Donald Athene M. , Lidzey David G. , Rodenburg Cornelia 
Published: April 2015

AbstractThe resolution capability of the scanning electron microscope has increased immensely in recent years, and is now within the sub-nanometre range, at least for inorganic materials. An equivalent advance has not yet been achieved for imaging the morphologies of nanostructured organic materials, such as organic photovoltaic blends. Here we show that energy-selective secondary electron detection can be used to obtain high-contrast, material-specific images of an organic photovoltaic blend. We also find that we can differentiate mixed phases from pure material phases in our data. The lateral resolution demonstrated is twice that previously reported from secondary electron imaging. Our results suggest that our energy-filtered scanning electron microscopy approach will be able to make major inroads into the understanding of complex, nano-structured organic materials.
Radiolysis as a solution for accelerated ageing studies of electrolytes in Lithium-ion batteries
Authors: Ortiz Daniel , Steinmetz Vincent , Durand Delphine , Legand Solène , Dauvois Vincent , Maître Philippe , Le Caër Sophie 
Published: April 2015

AbstractDiethyl carbonate and dimethyl carbonate are prototype examples of eco-friendly solvents used in lithium-ion batteries. Nevertheless, their degradation products affect both the battery performance and its safety. Therefore, it is of paramount importance to understand the reaction mechanisms involved in the ageing processes. Among those, redox processes are likely to play a critical role. Here we show that radiolysis is an ideal tool to generate the electrolytes degradation products. The major gases detected after irradiation (H2, CH4, C2H6, CO and CO2) are identified and quantified. Moreover, the chemical compounds formed in the liquid phase are characterized by different mass spectrometry techniques. Reaction mechanisms are then proposed. The detected products are consistent with those of the cycling of Li-based cells. This demonstrates that radiolysis is a versatile and very helpful tool to better understand the phenomena occurring in lithium-ion batteries.
Exposure age and ice-sheet model constraints on Pliocene East Antarctic ice sheet dynamics
Authors: Yamane Masako , Yokoyama Yusuke , Abe-Ouchi Ayako , Obrochta Stephen , Saito Fuyuki , Moriwaki Kiichi , Matsuzaki Hiroyuki 
Published: April 2015

AbstractThe Late Pliocene epoch is a potential analogue for future climate in a warming world. Here we reconstruct Plio-Pleistocene East Antarctic Ice Sheet (EAIS) variability using cosmogenic nuclide exposure ages and model simulations to better understand ice sheet behaviour under such warm conditions. New and previously published exposure ages indicate interior-thickening during the Pliocene. An ice sheet model with mid-Pliocene boundary conditions also results in interior thickening and suggests that both the Wilkes Subglacial and Aurora Basins largely melted, offsetting increased ice volume. Considering contributions from West Antarctica and Greenland, this is consistent with the most recent IPCC AR5 estimate, which indicates that the Pliocene sea level likely did not exceed +20 m on Milankovitch timescales. The inception of colder climate since ∼3 Myr has increased the sea ice cover and inhibited active moisture transport to Antarctica, resulting in reduced ice sheet thickness, at least in coastal areas.
Ddx1 knockout results in transgenerational wild-type lethality in mice
Authors: Hildebrandt Matthew R. , Germain Devon R. , Monckton Elizabeth A. , Brun Miranda , Godbout Roseline 
Published: April 2015

AbstractDEAD box 1 (DDX1) is a member of the DEAD box family of RNA helicases which are involved in all aspects of RNA metabolism. DDX1 has been implicated in a variety of biological processes, including 3’-end processing of mRNA, DNA repair, microRNA processing, tRNA maturation and mRNA transport. To study the role of DDX1 during development, we have generated mice carrying a constitutive Ddx1 knock-out allele. Ddx1+/− mice have no obvious phenotype and express similar levels of DDX1 as wild-type mice indicating compensation from the intact Ddx1 allele. Heterozygote matings produce no viable Ddx1−/− progeny, with Ddx1−/− embryos dying prior to embryonic day (E) 3.5. Intriguingly, the number of wild-type progeny is significantly decreased in heterozygote crosses, with two different heterozygote populations identified based on parental genotype: (i) normal Ddx1+/− mice which generate the expected number of wild-type progeny and (ii) Ddx1*/− mice (with * signifying a non-genetically altered allele) which generate a significantly reduced number of wild-type mice. The transgenerational inheritance of wild-type lethality observed upon crossing Ddx1*/− mice is independent of parental sex and occurs in cis through a mechanism that is different from other types of previously reported transgenerational epigenetic inheritance.