bluuebunny/crossref_metadata_2025_split huggingface dataset:
Following the money: copy-paste of lifestyle counseling documentation and provider billing
Authors: Zhang Mary , Shubina Maria , Morrison Fritha , Turchin Alexander 
Published: October 2013

Abstract

Background
Evidence suggests that copy-pasted components of electronic notes may not reliably reflect the care delivered. Federal agencies have raised concerns that such components may be used to justify inappropriately inflated claims for reimbursement. It is not known whether copied information is used to justify higher evaluation and management (E&M) charges.

Methods
This retrospective cohort study aimed to assess the relationship between the level of evaluation and management (E&M) charges and the method of documentation (none, distinct or copied) of lifestyle counseling (diet, exercise and weight loss) for patients with diabetes mellitus. To determine the association, an ordered multinomial logistic regression model that corrected for clustering within individual providers and patients and adjusted for patient and encounter characteristics was utilized. E&M charge level served as the primary outcome variable. Patients were included if they were followed by primary care physicians affiliated with two academic hospitals for a minimum of two years between 01/01/2000 and 12/13/2009.

Results
Lifestyle counseling was documented in 65.4% of 155,168 primary care encounters of 16,164 patients. Copied counseling was identified in 12,527 encounters. In multivariable analysis higher E&M charges were associated with older patient age, longer notes, treatment with insulin, medication changes and acute complaints. However, copied lifestyle counseling was associated with a decrease of 70.5% in the odds of higher E&M charge levels when time spent on counseling (required to justify higher charges based on counseling) was recorded (p<0.0001). This finding is opposite to what would have been expected if the impetus for copied documentation of lifestyle counseling was an increase in submitted E&M charges.

Conclusion
There is no evidence that copied documentation of lifestyle counseling is used to justify higher evaluation and management charges. Higher charges were generally associated with indicators of complexity of care.
Usefulness of metabolic syndrome score in the prediction of angiographic coronary artery disease severity according to the presence of diabetes mellitus: relation with inflammatory markers and adipokines
Authors: Kim Jong-Youn , Choi Eui-Young , Mun Hee-Sun , Min Pil-Ki , Yoon Young-Won , Lee Byoung Kwon , Hong Bum-Kee , Rim Se-Joong , Kwon Hyuck Moon 
Published: October 2013

Abstract

Background
It is a matter of debate whether metabolic syndrome (MS) improves cardiovascular risk prediction beyond the risk associated with its individual components. The present study examined the association of MS score with high sensitivity C-reactive protein (hs-CRP), interleukin-6 (IL-6), resistin, adiponectin, and angiographic coronary artery disease (CAD) severity according to the presence of DM. In addition, the predictive value of various clinical and biochemical parameters were analyzed, including the MS score for angiographic CAD.

Methods
The study enrolled 363 consecutive patients (196 men, 62 ± 11 years of age) who underwent coronary angiography for evaluation of chest pain. Blood samples were taken prior to elective coronary angiography. MS was defined by the National Cholesterol Education Program criteria, with MS score defined as the numbers of MS components. CAD was defined as > 50% luminal diameter stenosis of at least one major epicardial coronary artery. CAD severity was assessed using the Gensini score.

Results
Of the 363 patients studied, 174 (48%) had CAD and 178 (49%) were diagnosed with MS. When the patients were divided into 4 subgroups according to MS score (0–1, 2, 3, 4–5), IL-6 levels and the CAD severity as assessed by the Gensini score increased as MS scores increased. In contrast, adiponectin levels decreased significantly as MS scores increased. When subjects were divided into two groups according to the presence of DM, the relationships between MS score and IL-6, adiponectin, and Gensini score were maintained only in patients without DM. Age, smoking, DM, MS score, and adiponectin independently predicted angiographic CAD in the whole population. However, age is the only predictor for angiographic CAD in patients with DM.

Conclusions
In the presence of DM, neither adipokines nor MS score predicted angiographic CAD. However, in non-diabetic patients, IL-6 and adiponectin showed progressive changes according to MS score, and MS score was an independent predictor of CAD in patients without DM.
Pituitary apoplexy induced by Gonadotropin-releasing hormone agonists for treating prostate cancer-report of first Asian case
Authors: Huang Tsung-Yi , Lin Jih-Pin , Lieu Ann-Shung , Chen Yi-Ting , Chen Hung-Sheng , Jang Mei-Yu , Shen Jung-Tsung , Wu Wen-Jeng , Huang Shu-Pin , Juan Yung-Shun 
Published: October 2013

Abstract
We present the first Asian case of a 77-year-old man who developed pituitary apoplexy (PA) soon after gonadotropin-releasing hormone agonist (GnRHa) (leuprorelin) injection to treat prostate cancer. Headache, ophthalmoplegia, visual field deficit, nausea, and vomiting are the typical characteristics of pituitary apoplexy. Though the occurrence rate is rare, the consequence of this condition can vary from mild symptoms such as headache to life-threatening scenarios like conscious change. Magnetic resonance imaging is the best imaging modality to detect PA and sublabial trans-sphenoid pituitary tumor removal can resolve most of PA symptoms and is so far the best solution in consensus. We also review 11 previous reported cases receiving GnRHa for androgen deprivation therapy of prostate cancer, and hope to alert clinicians to use GnRHa with caution.
Saturable human neopterin response to interferon-α assessed by a pharmacokinetic-pharmacodynamic model
Authors: Jeon Sangil , Juhn Jae-Hyeon , Han Seunghoon , Lee Jongtae , Hong Taegon , Paek Jeongki , Yim Dong-Seok 
Published: October 2013

Abstract

Background
In this study, we developed a pharmacokinetic (PK)- pharmacodynamic (PD) model of a new sustained release formulation of interferon-α-2a (SR-IFN-α) using the blood concentration of IFN-α and neopterin in order to quantify the magnitude and saturation of neopterin production over time in healthy volunteers. The SR-IFN-α in this study is a solid microparticular formulation manufactured by spray drying of a feeding solution containing IFN-α, a biocompatible polymer (polyethylene glycol) and sodium hyaluronate.

Methods
The full PK and PD (neopterin concentration) datasets from 24 healthy subjects obtained after single doses of 9, 18, 27 and 36 MIU of subcutaneous SR-IFN-α were used to build the mixed-effect model using NONMEM (version 7.2) with the GFORTRAN compiler.

Results
A one-compartment model with first-order elimination and a mixture of zero- and first-order absorption was chosen to describe the PK of SR-IFN-α. The time-concentration profile of neopterin, the PD marker, was described by a turnover model combined with a single transit compartment. The saturable pattern of the neopterin response blurring the dose–response relationship of SR-IFN-α was addressed by introducing the concept of the EC50 increasing over time.

Conclusions
The PK-PD model of SR-IFN-α developed in this study has presented a quantitative tool to assess the time-course of a saturable neopterin response in humans.
Novel solutions for side information generation and fusion in multiview DVC
Authors: Petrazzuoli Giovanni , Cagnazzo Marco , Pesquet-Popescu Béatrice 
Published: October 2013

Abstract
One of the key problems in distributed video coding is the generation of side information. This task consists of producing an estimate of an image with some neighboring ones, such as those taken by the same camera at different time instants, or, in the case of multiview setups, images taken at the same time instant by different cameras. If both estimates are available, a further problem arises, which is how to merge them in order to create a single side information. This problem is very relevant since a good estimate of the unknown image will require only a few bits to be corrected. Considering a multiview distributed video-coding setup, we propose a novel technique for inter-view interpolation based on occlusion prediction, a new fusion technique from multiple estimates, and finally an adaptive validation step for switching among the three possible side information images: temporal, inter-view, and fusion. We provide a comprehensive set of experimental results, which indicate bit rate reductions of more than 9% in average; moreover, we observe much more consistent results with respect to state-of-the-art techniques.
A comprehensive map of the influenza A virus replication cycle
Authors: Matsuoka Yukiko , Matsumae Hiromi , Katoh Manami , Eisfeld Amie J , Neumann Gabriele , Hase Takeshi , Ghosh Samik , Shoemaker Jason E , Lopes Tiago JS , Watanabe Tokiko , Watanabe Shinji , Fukuyama Satoshi , Kitano Hiroaki , Kawaoka Yoshihiro 
Published: October 2013

AbstractBackgroundInfluenza is a common infectious disease caused by influenza viruses. Annual epidemics cause severe illnesses, deaths, and economic loss around the world. To better defend against influenza viral infection, it is essential to understand its mechanisms and associated host responses. Many studies have been conducted to elucidate these mechanisms, however, the overall picture remains incompletely understood. A systematic understanding of influenza viral infection in host cells is needed to facilitate the identification of influential host response mechanisms and potential drug targets.DescriptionWe constructed a comprehensive map of the influenza A virus (‘IAV’) life cycle (‘FluMap’) by undertaking a literature-based, manual curation approach. Based on information obtained from publicly available pathway databases, updated with literature-based information and input from expert virologists and immunologists, FluMap is currently composed of 960 factors (i.e., proteins, mRNAs etc.) and 456 reactions, and is annotated with ~500 papers and curation comments. In addition to detailing the type of molecular interactions, isolate/strain specific data are also available. The FluMap was built with the pathway editor CellDesigner in standard SBML (Systems Biology Markup Language) format and visualized as an SBGN (Systems Biology Graphical Notation) diagram. It is also available as a web service (online map) based on the iPathways+ system to enable community discussion by influenza researchers. We also demonstrate computational network analyses to identify targets using the FluMap.ConclusionThe FluMap is a comprehensive pathway map that can serve as a graphically presented knowledge-base and as a platform to analyze functional interactions between IAV and host factors. Publicly available webtools will allow continuous updating to ensure the most reliable representation of the host-virus interaction network. The FluMap is available athttp://www.influenza-x.org/flumap/.
Non-neoplastic bulky mediastinal mass presentation in an adolescent patient: a case report
Authors: Blatyta Paula Fraiman , Borba Claudio Carneiro , de Queiroz Ligia Reis , de Medeiros Raphael Salles Scortegagna , de Campos Fabiana Gomes , Bendit Israel 
Published: October 2013

Abstract

Introduction
Mediastinal masses in pediatric patients are very heterogeneous in origin and etiology. In the first decade of life, 70% of the mediastinal masses are benign whereas malignant tumors are more frequent in the second decade of life. Among the mediastinal masses, lymph nodes are the most common involved structures and could be enlarged due to a lymphoma, leukemia, metastatic disease, or due to infectious diseases as sarcoidosis, tuberculosis and others.

Case presentation
We report a case of a 13-year-old Caucasian girl who came to the emergency room with a history of intermittent fever, weight loss and night sweating for at least 1 month. A radiologic image work-up presented an anterior and posterior mediastinal mass. The 18F-fluorodeoxyglucose positron emission tomography presented a high maximum standard uptake value, which directed our decision for mediastinal biopsy for diagnostic elucidation. Histologic examination described the mass as granulomatous tuberculosis. The patient was treated with anti-tuberculosis therapy and developed a full clinical recovery.

Conclusions
The present case report demonstrates that a bulky mediastinal lymphadenopathy detected on 18F-fluorodeoxyglucose positron emission tomography is not always a malignant lesion, and in countries where tuberculosis is endemic, this etiology should not be forgotten during clinical investigations. There is a need for more accurate cut-off values for this technology; meanwhile, the further investigation of patients with bulky mediastinal masses with procedures such as the open biopsy is indispensable.
Selection and validation of reference genes for gene expression analysis in apomictic and sexual Cenchrus ciliaris
Authors: Simon Bindu , Conner Joann A , Ozias-Akins Peggy 
Published: October 2013

Abstract

Background
Apomixis is a naturally occurring asexual mode of seed reproduction resulting in offspring genetically identical to the maternal plant. Identifying differential gene expression patterns between apomictic and sexual plants is valuable to help deconstruct the trait. Quantitative RT-PCR (qRT-PCR) is a popular method for analyzing gene expression. Normalizing gene expression data using proper reference genes which show stable expression under investigated conditions is critical in qRT-PCR analysis. We used qRT-PCR to validate expression and stability of six potential reference genes (EF1alpha, EIF4A, UBCE, GAPDH, ACT2 and TUBA) in vegetative and reproductive tissues of B-2S and B-12-9 accessions of C. ciliaris.

Findings
Among tissue types evaluated, EF1alpha showed the highest level of expression while TUBA showed the lowest. When all tissue types were evaluated and compared between genotypes, EIF4A was the most stable reference gene. Gene expression stability for specific ovary stages of B-2S and B-12-9 was also determined. Except for TUBA, all other tested reference genes could be used for any stage-specific ovary tissue normalization, irrespective of the mode of reproduction.

Conclusion
Our gene expression stability assay using six reference genes, in sexual and apomictic accessions of C. ciliaris, suggests that EIF4A is the most stable gene across all tissue types analyzed. All other tested reference genes, with the exception of TUBA, could be used for gene expression comparison studies between sexual and apomictic ovaries over multiple developmental stages. This reference gene validation data in C. ciliaris will serve as an important base for future apomixis-related transcriptome data validation.
Whole-exome sequencing supports genetic heterogeneity in childhood apraxia of speech
Authors: Worthey Elizabeth A , Raca Gordana , Laffin Jennifer J , Wilk Brandon M , Harris Jeremy M , Jakielski Kathy J , Dimmock David P , Strand Edythe A , Shriberg Lawrence D 
Published: October 2013

AbstractBackgroundChildhood apraxia of speech (CAS) is a rare, severe, persistent pediatric motor speech disorder with associated deficits in sensorimotor, cognitive, language, learning and affective processes. Among other neurogenetic origins, CAS is the disorder segregating with a mutation inFOXP2in a widely studied, multigenerational London family. We report the first whole-exome sequencing (WES) findings from a cohort of 10 unrelated participants, ages 3 to 19 years, with well-characterized CAS.MethodsAs part of a larger study of children and youth with motor speech sound disorders, 32 participants were classified as positive for CAS on the basis of a behavioral classification marker using auditory-perceptual and acoustic methods that quantify the competence, precision and stability of a speaker’s speech, prosody and voice. WES of 10 randomly selected participants was completed using the Illumina Genome Analyzer IIx Sequencing System. Image analysis, base calling, demultiplexing, read mapping, and variant calling were performed using Illumina software. Software developed in-house was used for variant annotation, prioritization and interpretation to identify those variants likely to be deleterious to neurodevelopmental substrates of speech-language development.ResultsAmong potentially deleterious variants, clinically reportable findings of interest occurred on a total of five chromosomes (Chr3, Chr6, Chr7, Chr9 and Chr17), which included six genes either strongly associated with CAS (FOXP1andCNTNAP2) or associated with disorders with phenotypes overlapping CAS (ATP13A4,CNTNAP1,KIAA0319andSETX). A total of 8 (80%) of the 10 participants had clinically reportable variants in one or two of the six genes, with variants inATP13A4,KIAA0319andCNTNAP2being the most prevalent.ConclusionsSimilar to the results reported in emerging WES studies of other complex neurodevelopmental disorders, our findings from this first WES study of CAS are interpreted as support for heterogeneous genetic origins of this pediatric motor speech disorder with multiple genes, pathways and complex interactions. We also submit that our findings illustrate the potential use of WES for both gene identification and case-by-case clinical diagnostics in pediatric motor speech disorders.
Scale up and efficient bioethanol production involving recombinant cellulase (Glycoside hydrolase family 5) from Clostridium thermocellum
Authors: Das Saprativ P , Deka Deepmoni , Ghosh Arabinda , Das Debasish , Jawed Mohammad , Goyal Arun 
Published: October 2013

Abstract

Background
Lignocellulose degrading fungal enzymes have been in use at industrial level for more than three decades. However, the main drawback is the high cost of the commercially available Trichoderma reesei cellulolytic enzymes.

Results
The hydrolytic performance of a novel Clostridium thermocellum cellulolytic recombinant cellulase expressed in Escherichia coli cells was compared with the naturally isolated cellulases in different modes of fermentation trials using steam explosion pretreated thatch grass and Zymomonas mobilis. Fourier transform infrared (FT-IR) spectroscopic analysis confirmed the efficiency of steam explosion pretreatment in significant release of free glucose moiety from complex lignocellulosic thatch grass. The recombinant GH5 cellulase with 1% (w v-1) substrate and Z. mobilis in shake flask separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) trials demonstrated highest ethanol titre (0.99 g L-1, 1.2 g L-1) as compared to Bacillus subtilis (0.51 g L-1, 0.72 g L-1) and Trichoderma reesei (0.67 g L-1, 0.94 g L-1). A 5% (w v-1) substrate with recombinant enzyme in shake flask SSF resulted in a 7 fold increment of ethanol titre (8.8 g L-1). The subsequent scale up in a 2 L bioreactor with 1 L working volume yielded 16.13 g L-1 ethanol titre implying a 2 fold upturn. The rotary evaporator based product recovery from bioreactor contributed 94.4 (%, v v-1) pure ethanol with purification process efficiency of 22.2%.

Conclusions
The saccharification of steam exploded thatch grass (Hyparrhenia rufa) by recombinant cellulase (GH5) along with Z. mobilis in bioethanol production was studied for the first time. The effective pretreatment released substantial hexose sugars from cellulose as confirmed by FT-IR studies. In contrast to two modes of fermentation, SSF processes utilizing recombinant C. thermocellum enzymes have the capability of yielding a value-added product, bioethanol with the curtailment of the production costs in industry.
Microbial community composition of Tirez lagoon (Spain), a highly sulfated athalassohaline environment
Authors: Montoya Lilia , Vizioli Carlotta , Rodríguez Nuria , Rastoll María José , Amils Ricardo , Marin Irma 
Published: October 2013

AbstractBackgroundThe aim was to study the seasonal microbial diversity variations of an athalassohaline environment with a high concentration of sulfates in Tirez lagoon (La Mancha, Spain). Despite the interest in these types of environments there is scarce information about their microbial ecology, especially on their anoxic sediments.ResultsWe report the seasonal microbial diversity of the water column and the sediments of a highly sulfated lagoon using both molecular and conventional microbiological methods. Algae andCyanobacteriawere the main photosynthetic primary producers detected in the ecosystem in the rainy season. Also dinoflagelates and filamentous fungi were identified in the brines. The highest phylotype abundance in water and sediments corresponded to members of the bacterial phylumProteobacteria, mainly of theGamma-andAlphaproteobacteriaclasses.FirmicutesandActinobacteriawere isolated and identified in Tirez brines and sediment samples. Halophilic sulfate reducingDeltaproteobacteriawere also detected (Desulfohalobium).ConclusionsImportant differences have been found in the microbial diversity present in the Tirez water column and the sediments between the wet and dry seasons. Also the Tirez lagoon showed a high richness of the bacterialAlpha-andDeltaproteobacteria,Bacteroidetes,Firmicutes,Actinobacteriaand for the archaealEuryarchaeota.
Monoclonal antibody 10A5 recognizes an antigen unique to the water-insoluble 25/45 membrane fraction of the rat ocular lens
Authors: Whitman Joseph K , Alviar Abigail F , Fleschner Charles R , Stuart Melissa K 
Published: October 2013

Abstract
The water-insoluble 25/45 fraction and non-sedimenting membrane fraction (NSMF) are two membrane preparations isolated from the ocular lens. The fractions are postulated to represent distinct subdomains of the lens with unique functions. However, attempts to distinguish between the two fractions by detecting proteins present in one fraction but absent from other have been unsuccessful. In this study, we exploited the ability of the mouse immune system to detect antigenic differences between the 25/45 fraction and NSMF isolated from the lenses of 20-day-old rats. We generated a monoclonal antibody (MAb 10A5) that reacts with a ganglioside-like antigen that is present in the 25/45 fraction but absent from the NSMF. Restriction of the antigen to the 25/45 fraction in 20-day-old animals supports the hypothesis that the 25/45 fraction and NSMF represent different subdomains within the ocular lens.
Oxidation of Dihedral-angle-controlled 2,2′-Bithiophenes with Terminal Ferrocenyl Groups
Authors: Sato Masa-aki , Arita Syuhei , Kawajiri Kazuki , Isayama Atsushi 
Published: October 2013

Abstract
Novel dihedral-angle-controlled 2,2′-bithiophenes with terminal ferrocenyl groups have been designed and prepared. The electrochemical and spectroscopic properties indicated that the interaction between the terminal ferrocene moieties was distinctly affected by the twist in the π-conjugated system.