bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical and genetic analysis of Taiwanese patients with hereditary spastic paraplegia type 5
Authors: Lan M.‐Y. , Yeh T.‐H. , Chang Y.‐Y. , Kuo H.‐C. , Sun H. S. , Lai S.‐C. , Lu C.‐S. 
Published: March 2014

Background and purposeSpastic paraplegia type 5 (SPG5) is an autosomal recessive (AR) hereditary spastic paraplegia (HSP) associated with pure or complicated phenotypes. This study aimed to screen SPG5 in Taiwanese HSP patients.MethodsSequencing of the SPG5 gene, CYP7B1, was performed in a cohort of 25 ethnic Han Taiwanese patients with AR or sporadic HSP. Clinical information and magnetic resonance imaging (MRI) were analyzed in confirmed SPG5 patients.ResultsOne (33%) AR kindred and four (18%) sporadic cases had CYP7B1 mutations. All of the SPG5 cases carried the mutation c.334 C>T (R112X). Haplotype analysis suggested a ‘founder effect’ in ethnic Hans for this mutation. The phenotype was either pure or complicated by cerebellar ataxia. For the primary HSP phenotype, there were profound dorsal column sensory deficits in all patients. Spine MRI showed thoraco‐lumbar cord atrophy in some patients.ConclusionsSpastic paraplegia type 5 is a common cause of AR and sporadic HSPs that has a higher frequency in Taiwanese than in other ethnic groups. It is associated with a CYP7B1 founder mutation and its phenotype is characterized by pronounced dorsal column sensory loss, with cerebellar ataxia in some patients.
Real‐time spatial management approaches to reduce bycatch and discards: experiences from Europe and the United States
Authors: Little Alyson S , Needle Coby L , Hilborn Ray , Holland Daniel S , Marshall C Tara 
Published: March 2014

AbstractSpatial management measures are currently being used to manage bycatch and discards, given the spatial heterogeneity of fish distributions. However, permanent fishing closures are often poorly implemented, unresponsive to stock dynamics and do not achieve their management objectives. Recently, real‐time spatial management tools for managing bycatch and discards implemented under either a comanagement or self‐governance approach have been introduced in Europe and theUS. Real‐time catch and discard information is shared among fishers to incentivise and encourage vessels to leave areas of high bycatch. Here, the similarities and differences, in governance, implementation and management of ten real‐time spatial management systems from across Europe and theUSare reviewed. A framework is developed to characterize the attributes associated with voluntary, private and regulatory real‐time spatial management tools. Challenges and management practices in the different case studies are reviewed providing insights for designing these spatial management tools. The results illustrate that real‐time spatial management approaches can create incentives for fishers to develop, use and share information and technology to avoid undesired catch. Compared with Europe, theUShas developed spatial management tools with more truly real‐time mechanisms and with greater involvement of the fishing industry in designing and operating the tools.
Climate‐driven spatial mismatches between British orchards and their pollinators: increased risks of pollination deficits
Authors: Polce Chiara , Garratt Michael P , Termansen Mette , Ramirez‐Villegas Julian , Challinor Andrew J , Lappage Martin G , Boatman Nigel D , Crowe Andrew , Endalew Ayenew Melese , Potts Simon G , Somerwill Kate E , Biesmeijer Jacobus C 
Published: March 2014

AbstractUnderstanding how climate change can affect crop‐pollinator systems helps predict potential geographical mismatches between a crop and its pollinators, and therefore identify areas vulnerable to loss of pollination services. We examined the distribution of orchard species (apples, pears, plums and other top fruits) and their pollinators in Great Britain, for present and future climatic conditions projected for 2050 under the SRES A1B Emissions Scenario. We used a relative index of pollinator availability as a proxy for pollination service. At present, there is a large spatial overlap between orchards and their pollinators, but predictions for 2050 revealed that the most suitable areas for orchards corresponded to low pollinator availability. However, we found that pollinator availability may persist in areas currently used for fruit production, which are predicted to provide suboptimal environmental suitability for orchard species in the future. Our results may be used to identify mitigation options to safeguard orchard production against the risk of pollination failure in Great Britain over the next 50 years; for instance, choosing fruit tree varieties that are adapted to future climatic conditions, or boosting wild pollinators through improving landscape resources. Our approach can be readily applied to other regions and crop systems, and expanded to include different climatic scenarios.
Sustainability of forest bioenergy in Europe: land‐use‐related carbon dioxide emissions of forest harvest residues
Authors: Repo Anna , Böttcher Hannes , Kindermann Georg , Liski Jari 
Published: March 2014

AbstractIncreasing bioenergy production from forest harvest residues decreases litter input to the soil and can thus reduce the carbon stock and sink of forests. This effect may negate greenhouse gas savings obtained by using bioenergy. We used a spatially explicit modelling framework to assess the reduction in the forest litter and soil carbon stocks across Europe, assuming that a sustainable potential of bioenergy from forest harvest residues is taken into use. The forest harvest residue removal reduced the carbon stocks of litter and soil on average by 3% over the period from 2016 to 2100. The reduction was small compared to the size of the carbon stocks but significant in comparison to the amount of energy produced from the residues. As a result of these land‐use‐related emissions, bioenergy production from forest harvest residues would need to be continued for 60–80 years to achieve a 60% carbon dioxide (CO2) emission reduction in heat and power generation compared to the fossil fuels it replaces in most European countries. The emission reductions achieved and their timings varied among countries because of differences in the litter and soil carbon loss. Our results show that extending the current sustainability requirements for bioliquids and biofuels to solid bioenergy does not guarantee efficient reductions in greenhouse gas emissions in the short‐term. In the longer‐term, bioenergy from forest harvest residues may pave the way to low‐emission energy systems.
Feasibility and Efficacy of Simultaneous Pulmonary Vein Isolation and Cavotricuspid Isthmus Ablation Using Cryotherapy
Authors: DHILLON PARAMDEEP S. , DOMENICHINI GIULIA , GONNA HANNEY , BASTIAENEN RACHEL , NORMAN MARK , GALLAGHER MARK M. 
Published: March 2014

Cryotherapy for Simultaneous CTI Ablation and PVIIntroductionPulmonary vein isolation (PVI) and cavotricuspid isthmus (CTI) ablation are often performed as part of the same procedure. In many cases, PVI is performed by cryotherapy and then CTI ablation by radiofrequency (RF) energy. We sought to determine whether it is more efficient to perform CTI ablation simultaneously with PVI using separate cryogenerators.Methods and ResultsWe performed cryoablation of the CTI during PVI with the Arctic Front cryoballoon in 25 consecutive patients with clinical indications for both (PVI/CTI‐cryo group). Procedural data were compared to those of 25 matched patients who underwent PVI only by the same operator (PVI‐only group), and 25 patients who underwent PVI by cryotherapy and CTI ablation using RF energy sequentially during the same procedure (PVI/CTI‐mixed group). No complication occurred. All veins were isolated; bidirectional CTI block was demonstrated in all cases where it was attempted, except for 1 patient in the PVI/CTI‐mixed group. Procedure and fluoroscopy duration were significantly shorter in the PVI/CTI‐cryo group (162 ± 34 and 24 ± 5 minutes) than in the PVI/CTI‐mixed group (209 ± 46 minutes, P < 0.001 and 59 ± 28 minutes, P < 0.001). Procedure and fluoroscopy duration in the PVI‐only group (155 ± 32 and 22 ± 8 minutes) were similar to those in the PVI/CTI‐cryo group (P = NS) but significantly shorter than in the PVI/CTI‐mixed group (P < 0.001 for both). Clinical outcomes were similar in all groups.ConclusionWhen CTI ablation is performed with RF energy after PVI by cryoballoon, it adds significantly to the procedure and fluoroscopy durations; when performed contemporaneously using cryotherapy at both sites, the procedure and fluoroscopy durations are not prolonged.
Improved repair of dermal wounds in mice lacking microRNA‐155
Authors: van Solingen Coen , Araldi Elisa , Chamorro‐Jorganes Aranzazu , Fernández‐Hernando Carlos , Suárez Yajaira 
Published: March 2014

AbstractWound healing is a well‐regulated but complex process that involves haemostasis, inflammation, proliferation and maturation. Recent reports suggest that microRNAs (miRs) play important roles in dermal wound healing. In fact, miR deregulation has been linked with impaired wound repair. miR‐155 has been shown to be induced by inflammatory mediators and plays a central regulatory role in immune responses. We have investigated the potential role of miR‐155 in wound healing. By creating punch wounds in the skin of mice, we found an increased expression of miR‐155 in wound tissue when compared with healthy skin. Interestingly, analysis of wounds of mice lacking the expression of miR‐155 (miR‐155−/−) revealed an increased wound closure when compared with wild‐type animals. Also, the accelerated wound closing correlated with elevated numbers of macrophages in wounded tissue. Gene expression analysis of wounds tissue and macrophages isolated from miR‐155−/− mice that were treated with interleukin‐4 demonstrated an increased expression of miR‐155 targets (BCL6, RhoA and SHIP1) as well as, the finding in inflammatory zone‐1 (FIZZ1) gene, when compared with WT mice. Moreover, the up‐regulated levels of FIZZ1 in the wound tissue of miR‐155−/− mice correlated with an increased deposition of type‐1 collagens, a phenomenon known to be beneficial in wound closure. Our data indicate that the absence of miR‐155 has beneficial effects in the wound healing process.
XBP1S, a BMP2‐inducible transcription factor, accelerates endochondral bone growth by activating GEP growth factor
Authors: Guo Feng‐Jin , Xiong Zhangyuan , Han Xiaofeng , Liu Chuanju , Liu Yanna , Jiang Rong , Zhang Peng 
Published: March 2014

AbstractWe previously reported that transcription factor XBP1S binds to RUNX2 and enhances chondrocyte hypertrophy through acting as a cofactor of RUNX2. Herein, we report that XBP1S is a key downstream molecule of BMP2 and is required for BMP2‐mediated chondrocyte differentiation. XBP1S is up‐regulated during chondrocyte differentiation and demonstrates the temporal and spatial expression pattern during skeletal development. XBP1S stimulates chondrocyte differentiation from mesenchymal stem cells in vitro and endochondral ossification ex vivo. In addition, XBP1S activates granulin‐epithelin precursor (GEP), a growth factor known to stimulate chondrogenesis, and endogenous GEP is required, at least in part, for XBP1S‐stimulated chondrocyte hypertrophy, mineralization and endochondral bone formation. Furthermore, XBP1S enhances GEP‐stimulated chondrogenesis and endochondral bone formation. Collectively, these findings demonstrate that XBP1S, a BMP2‐inducible transcription factor, positively regulates endochondral bone formation by activating GEP chondrogenic growth factor.
Diagnostic and prognostic role of SCCA‐IgM serum levels in hepatocellular carcinoma (HCC)
Authors: Pozzan Caterina , Cardin Romilda , Piciocchi Marika , Cazzagon Nora , Maddalo Gemma , Vanin Veronica , Giacomin Anna , Pontisso Patrizia , Cillo Umberto , Farinati Fabio 
Published: March 2014

AbstractBackground and AimThe serpin squamous cell carcinoma antigen complexed with IgM (SCCA‐IgM) has been reported as a promising serological marker for hepatocellular carcinoma (HCC). We aimed to further evaluate SCCA‐IgM diagnostic accuracy and to determine its prognostic role.MethodsSCCA‐IgM levels were determined in 327 sera obtained from 81 HCC patients, 206 cirrhotics and 40 healthy blood donors (controls). Sensitivity, specificity, correlation with clinical and tumor parameters and with survival were evaluated.ResultsHCC patients had SCCA‐IgM levels significantly higher than controls and cirrhotics (P < 0.0001). Sensitivity, specificity, positive and negative predictive values for HCC were 89%, 50%, 41% and 92%, respectively. In comparison, sensitivity and specificity for alphafetoprotein were 48% and 85%. SCCA‐IgM levels were not significantly correlated with clinical or biological variables. With a cut‐off of 130 AU/mL (receiver operating characteristic curves), SCCA‐IgM proved efficient in the prediction of prognosis, identifying the patients with long overall survival (efficiency validated in the homogenous subgroup of patients with intermediate‐stage HCC undergoing transarterial chemoembolization) and predicting progression‐free survival. A Cox multivariate analysis confirmed SCCA‐IgM predictive value, identifying tumor size and SCCA‐IgM levels as independent predictors of survival. A reduction in SCCA‐IgM levels correlated with response to treatment.ConclusionsSCCA‐IgM is a sensitive marker of HCC in patients with cirrhosis even though lacking in specificity. The determination of the levels of the marker in HCC patients is highly efficient in predicting the patients' prognosis, identifying those with long overall and progression‐free survival and the responders and should be introduced in the clinical practice.
Assessment of efficacy of platelet‐rich plasma (PRP) on infraorbital dark circles and crow's feet wrinkles
Authors: Mehryan Pedram , Zartab Hamed , Rajabi Ali , Pazhoohi Neda , Firooz Alireza 
Published: March 2014

SummaryBackgroundInfraorbital skin hyperpigmentation, commonly called dark circles, and crow's feet wrinkles are common cosmetic concerns. Various methods of treatment have been evaluated with variable outcomes.ObjectiveThis study was performed to assess the efficacy of platelet‐rich plasma (PRP) injection for treating periorbital dark circles and crow's feet.MethodsTen participants with a mean age of 41.2 years were treated in a single session with intradermal injections of 1.5 mL PRP into tear trough area and crow's feet wrinkles on each side. The effects on melanin content, color homogeneity of the treated area, epidermal stratum corneum hydration, and wrinkle volume and visibility index were compared 3 months after treatment with baseline. Physician's global assessment and participants' satisfaction and any potential side effects were also assessed.ResultsThe improvement in infraorbital color homogeneity was statistically significant (P = 0.010), but no statistically significant changes were observed in melanin content, stratum corneum hydration, wrinkle volume, and visibility index. Participant's satisfaction score and physician's global assessment score were 2.2 and 1.7, respectively, on a 0–3 scale.ConclusionPlatelet‐rich plasma may have the potential to improve infraorbital dark circle in terms of color homogeneity of the region, though this remains to be proven using larger, controlled studies using multiple injections.
Contact sensitization to cosmetic series of allergens in a general population in Beijing
Authors: Zhao Jian , Li Lin‐feng 
Published: March 2014

SummaryCosmetic allergic contact dermatitis (CACD) due to common cosmetic allergens in standard series has been extensively studied; however, the prevalence of contact allergy to other cosmetic allergens other than those in standard series is largely unknown. In this study, the frequency of contact sensitization to a European cosmetic series of allergens (Chemotechnique Diagnostics, Vellinge, Sweden) in healthy university student volunteers were detected in Beijing. Of 201 students studied, fifty‐eight exhibited positive results, and 9 of them reported had cosmetics related dermatitis previously. The total positivity rate was not correlated to gender. The leading allergens were thimerosal (19.4%), shellac (3.0%), cocamidopropyl betaine (2.0%), hexamethylenetetramine (1.5%), dodecyl gallate (1.5%), hexahydro‐1,3,5‐tris‐(2‐hydroxyethyl)triazine (1.0%) and methyldibromo glutaronitrile (1.0%). The positivity rate of thimerosal patch test in men (9.8%) was lower than that of women (23.6%, P < 0.05, Chi square test), but no difference could be found between the prevalence of other cosmetic allergens in men and women (P > 0.05, Chi square test). These results suggested that some cosmetic‐related contact allergies may be missed by just testing patients with the European standard series or T.R.U.E. test system only, we recommend shellac, cocamidopropyl betaine, hexamethylenetetramine and dodecyl gallate as the additionally candidates for patch testing in patients with suspected CACD.
Fish oil supplementation induces expression of genes related to cell cycle, endoplasmic reticulum stress and apoptosis in peripheral blood mononuclear cells: a transcriptomic approach
Authors: Myhrstad M. C. W. , Ulven S. M. , Günther C.‐C. , Ottestad I. , Holden M. , Ryeng E. , Borge G. I. , Kohler A. , Brønner K. W. , Thoresen M. , Holven K. B. 
Published: March 2014

AbstractBackgroundFish oil supplementation has been shown to alter gene expression of mononuclear cells both in vitro and in vivo. However, little is known about the total transcriptome profile in healthy subjects after intake of fish oil. We therefore investigated the gene expression profile in peripheral blood mononuclear cells (PBMCs) after intake of fish oil for 7 weeks using transcriptome analyses.DesignIn a 7‐week, double‐blinded, randomized, controlled, parallel‐group study, healthy subjects received 8 g day−1 fish oil (1.6 g day−1 eicosapentaenoic acid + docosahexaenoic acid) (n = 17) or 8 g day−1 high oleic sunflower oil (n = 19). Microarray analyses of RNA isolated from PBMCs were performed at baseline and after 7 weeks of intervention.ResultsCell cycle, DNA packaging and chromosome organization are biological processes found to be upregulated after intake of fish oil compared to high oleic sunflower oil using a moderated t‐test. In addition, gene set enrichment analysis identified several enriched gene sets after intake of fish oil. The genes contributing to the significantly different gene sets in the subjects given fish oil compared with the control group are involved in cell cycle, endoplasmic reticulum (ER) stress and apoptosis. Gene transcripts with common motifs for 35 known transcription factors including E2F, TP53 and ATF4 were upregulated after intake of fish oil.ConclusionWe have shown that intake of fish oil for 7 weeks modulates gene expression in PBMCs of healthy subjects. The increased expression of genes related to cell cycle, ER stress and apoptosis suggests that intake of fish oil may modulate basic cellular processes involved in normal cellular function.
Occurrence of Ralstonia solanacearum Race 2 Biovar 1 Associated with Moko Disease of Banana (Musa paradisiaca cv. Nipah) in Malaysia
Authors: Zulperi Dzarifah , Sijam Kamaruzaman , Mior Ahmad Zainal A , Awang Yahya , Sulaiman Rashid Tavga 
Published: March 2014

AbstractDuring June 2011 to March 2012, Moko disease symptoms were observed in banana cv. Nipah in two Malaysian states. The primer pairs ISRso19F/ISRso19R were used for defined identification of Ralstonia solanacearum race 2 strain. PCR amplification of all isolates produced a 1900 amplicon and exhibited 93% phylogenetic similarity with reference strain (AF450275). Based on symptoms, biochemical tests, pathogenicity assay, molecular and phylogenetic studies, we concluded that the isolated bacterium was R. solanacearum race 2 biovar 1.
Successful management of hepatic mucormycosis in an acute lymphoblastic leukaemia patient: a case report and review of the literature
Authors: Tuysuz Gulen , Ozdemir Nihal , Senyuz Osman Faruk , Emre Senol , Kantarcioglu Serda , Adaletli İbrahim , Kepil Nuray , Tutuncu Çigdem , Celkan Tiraje 
Published: March 2014

SummaryWe present a case of hepatic mucormycosis in a 9‐year‐old boy with acute lymphoblastic leukaemia. Despite long‐term use of combined liposomal amphotericin B and posaconazole therapy, the lesion persisted and could only be treated by surgical excision. After surgery, antifungal treatment was continued with posaconazole. On follow‐up, the patient had two episodes of ascending cholangitis which were responsive to intravenous antibiotics. He is doing well at the moment in remission for 2.5 years. Mucormycosis was long regarded as a fatal infection with poor prognosis. With early medical and surgical management, survival rates increase. Isolated hepatic mucormycosis is rare and only seven cases were reported in the literature up to now. We wanted to emphasise the role of early surgery in patients with hepatic mucormycosis in view of the literature.
Cost effectiveness of caspofungin vs. voriconazole for empiric therapy in Turkey
Authors: Turner S. J. , Senol E. , Kara A. , Al‐Badriyeh D. , Dinleyici E. C. , Kong D. C. M. 
Published: March 2014

SummaryInvasive fungal infections from febrile neutropenia are associated with significant cost and mortality. The mainstay of treatment has been liposomal amphotericin B, however, echinocandins and azoles have shown promise as alternative treatments. Data on clinical efficacy exist, however, data incorporating pharmacoeconomic considerations are required in Turkey. The aim of this study was to investigate the cost effectiveness of caspofungin vs. voriconazole in empiric treatment of febrile neutropenia in Turkey. A decision analytic model was utilised, built upon two randomised‐controlled trials and supplemented with expert panel input from clinicians in Turkey. A five‐point composite outcome measure was utilised and sensitivity analyses were performed to demonstrate the robustness of the model. The base case scenario resulted in caspofungin being preferred by TL2,533, TL29,256 and TL2,536 per patient treated, successfully treated patient and patient survival, respectively (approx. USD1414, 16 328 and 1415); sensitivity analyses did not change the outcome. Monte Carlo simulation highlighted a 78.8% chance of favouring caspofungin. The result was moderately sensitive to treatment duration and acquisition cost of the antifungal agents compared. This is the first pharmacoeconomic study comparing caspofungin to voriconazole within Turkey, resulting in an advantage towards caspofungin. The study will aid in formulary decision‐making based on the clinical and economic consequences of each agent in the Turkish health care setting.
Psychometric properties of theChinese‐versionQuality ofNursingWorkLifeScale
Authors: Lee Ya‐Wen , Dai Yu‐Tzu , McCreary Linda L. , Yao Grace , Brooks Beth A. 
Published: March 2014

AbstractIn this study, we developed and tested the psychometric properties of theChinese‐versionQuality ofNursingWorkLifeScale along seven subscales: supportive milieu with security and professional recognition, work arrangement and workload, work/home life balance, head nurse's/supervisor's management style, teamwork and communication, nursing staffing and patient care, and milieu of respect and autonomy. An instrument‐development procedure with three phases was conducted in seven hospitals in 2010–2011. Phase I comprised translation and the cultural‐adaptation process, phase II comprised a pilot study, and phase III comprised a field‐testing process. Purposive sampling was used in the pilot study (n = 150) and the large field study (n = 1254). Five new items were added, and 85.7% of the original items were retained in the 41 item Chinese version. Principal component analysis revealed that a model accounted for 56.6% of the variance with acceptable internal consistency, concurrent validity, and discriminant validity. This study gave evidence of reliability and validity of the 41 itemChinese‐versionQuality ofNursingWorkLifeScale.