bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fruit pit hardening: physical measurement during olive fruit growth
Authors: Rapoport H.F. , Pérez‐López D. , Hammami S.B.M. , Agüera J. , Moriana A. 
Published: June 2013

AbstractIn drupe‐type fruits, pit hardening, resulting from sclerification of the fruit endocarp, is widely used as a phenological marker for both physiological studies and orchard management. In spite of the importance of pit hardening for understanding fruit development processes and for agricultural practices, however, its quantification has remained obscure and precision has been lost with time and lax usage. In this study, we used a mechanical device to measure the physical pressure required to break the olive pit in order to define the timing of pit hardening more precisely and to permit closer observation of its relationship to fruit and endocarp growth and development. Over 4 years we found that pit‐hardening pressure increased following a sigmoid pattern, at first gradually but then with a large and rapid increment of change in a relatively short period of time. The rapid acceleration of hardening began at the time when pit longitudinal and transverse diameters attained their maximum size. That timing is consistent with the anatomical differentiation of the sclerified endocarp cells which can no longer expand nor divide. The results improve our knowledge of pit hardening and provide a more precise context for evaluating the metabolic costs, physiological interactions and genetic controls of stone fruit endocarp development. On a practical level, the association of the intensification of pit‐breaking pressure with the cessation of pit expansion indicates that pit diameters can be useful morphological markers to identify the onset of this period.
Non‐antibiotic properties of tetracyclines and their clinical application in dermatology
Authors: Perret Leanna J , Tait Clare P 
Published: June 2013

AbstractDermatologists have used tetracyclines since the 1950s to treat disorders that do not necessarily have an infectious aetiology. Their anti‐inflammatory and anti‐collagenase properties contribute significantly to their success in treating diseases such as rosacea and acne. This article reviews the non‐antibiotic properties of tetracyclines and their clinical application in dermatology.
Retrospective chart review in a cohort of patients with urticarial dermatitis
Authors: Banan Parastoo , Butler Gregory , Wu Jason 
Published: June 2013

AbstractUrticarial dermatitis is a poorly understood skin condition while it seems to be much more common than the paucity of reports suggest. It manifests with severely pruritic papules and plaques that resemble eczematous and urticarial lesions morphologically. The key clues to diagnosis are the urticarial appearance and overlap with an eczematous reaction. Here, we present a series of 19 cases (13 women and six men) with urticarial dermatitis clinically and histologically. The patients' average age was 58 and most of the cases were idiopathic. Trunk and proximal extremities were the most common sites involved followed by the distal extremities. Poor response to potent topical corticosteroids and antihistamines was usual and many patients required oral prednisone or other immunosuppressant agents or phototherapy.
Polymorphic variants of CYP17 and CYP19A and risk of infertility in endometriosis
Authors: Szczepańska Malgorzata , Wirstlein Przemyslaw , Skrzypczak Jana , Jagodziński Paweł P. 
Published: June 2013

AbstractObjectiveEndometriosis is recognized as an estrogen‐dependent disease. There are conflicting data demonstrating single nuclear polymorphisms (SNPs) of CYP17 and CYP19 steroidogenic genes as related to endometriosis risk. We assessed the CYP17 5′‐untranslated region ‐34 A/G (rs743572) and CYP19 Ex10 + C1558T (rs10046) SNPs in stage I–II endometriosis.DesignCase–control study.SettingDivision of reproduction at a university department in Poland.PopulationA total of 115 women with diagnosed stage I–II endometriosis according to the revised American Society for Reproductive Medicine (rASRM) classification and 197 fertile women as controls.MethodsThe SNPs CYP17 ‐34 A/G and CYP19 Ex10 + C1558T were identified by high‐resolution melting curve analysis.Main outcome measuresGenotype prevalence and odds ratio for recessive and dominant genetic model for CYP17 and CYP19 SNPs.ResultsWe observed a significantly increased CYP17 GG and GA genotype frequency in women diagnosed with rASRM stage I–II endometriosis compared with fertile women (OR = 2.4; 95% CI 1.4–4.2, p = 0.002). We also found a significantly increased CYP17 G allele frequency in cases compared with controls (OR = 1.6; 95% CI 1.2–2.2, p = 0.004). There were no significant differences in the distribution of the CYP17 GG genotype and CYP19 Ex10 + C1558T polymorphism between women diagnosed with rASRM stage I–II endometriosis and controls.ConclusionThe CYP17 ‐34 G variant, previously associated with increased 17β‐estradiol production, displayed a contribution to stage I–II endometriosis in women from a Polish population. Increased 17β‐estradiol concentration in carriers of the CYP17 ‐34 G variant might contribute to endometriosis and associated pathological processes.
Why is intelligence associated with stability of happiness?
Authors: Kanazawa Satoshi 
Published: June 2013

In the National Child Development Study, life‐course variability in happiness over 18 years was significantly negatively associated with its mean level (happier individuals were more stable in their happiness, and it was not due to the ceiling effect), as well as childhood general intelligence and all Big Five personality factors (except for Agreeableness). In a multiple regression analysis, childhood general intelligence was the strongest predictor of life‐course variability in life satisfaction, stronger than all Big Five personality factors, including Emotional stability. More intelligent individuals were significantly more stable in their happiness, and it was not entirely because: (1) they were more educated and wealthier (even though they were); (2) they were healthier (even though they were); (3) they were more stable in their marital status (even though they were); (4) they were happier (even though they were); (5) they were better able to assess their own happiness accurately (even though they were); or (6) they were better able to recall their previous responses more accurately or they were more honest in their survey responses (even though they were both). While I could exclude all of these alternative explanations, it ultimately remained unclear why more intelligent individuals were more stable in their happiness.
Investigation of the homogeneity of methacrylate allergens in commercially available patch test preparations
Authors: Mose Kristian Fredløv , Andersen Klaus Ejner , Christensen Lars Porskjær 
Published: June 2013

SummaryBackgroundThe homogeneity of methacrylates in commercial patch test preparations has not yet been investigated. Inhomogeneous patch test preparations may give rise to false‐negative or false‐positive patch test results in patients suspected of having methacrylate allergy.ObjectivesTo investigate the homogeneity of methacrylates in commercial patch test preparations.MethodsFresh commercial patch test preparations of methyl methacrylate (MMA) and 2‐hydroxyethyl methacrylate (2‐HEMA) from three test material suppliers in Europe were analysed quantitatively by means of normal‐phase high‐performance liquid chromatography.ResultsThe initial concentration of MMA in all six patch test preparations was lower than stated on the label, whereas four of six patch test preparations of 2‐HEMA were in accordance with the stated concentrations. The concentration of MMA increased markedly from the top segment close to the tip of the syringe to the bottom segment adjacent to the piston in four syringes (3–6). In contrast, syringes with 2‐HEMA maintained a constant concentration throughout the test preparation, apart from two syringes (11 and 12).ConclusionVariations in concentration and heterogeneous distribution of MMA and 2‐HEMA in patch test preparations may be an additional cause of variation in patch test results, besides other technical details and reading.
Validating a screening tool for mental health and substance use risk in an Indigenous prison population
Authors: Ober Coralie , Dingle Kaeleen , Clavarino Alexandra , Najman Jake M. , Alati Rosa , Heffernan Edward B. 
Published: June 2013

AbstractIntroduction and AimsThe Indigenous Risk Impact Screen (IRIS) is a validated culturally appropriate and widely used tool in the community for assessing substance use and mental disorder. This research aimed to assess the utility of this tool in an Indigenous prison population.Design and MethodsThe study used data collected from a cross‐sectional study of mental health among Indigenous inmates in Queensland custodial centres (n = 395, 84% male). Participants were administered a modified version of the IRIS and International Classification of Diseases‐10 diagnoses of substance use, depressive and anxiety disorders obtained using the Composite International Diagnostic Interview (CIDI). The concurrent validity of the modified IRIS was assessed against those of the CIDI.ResultsThree hundred and twelve people screened as high risk for a substance use disorder and 179 were high risk for mental problems. There were 73% of males and 88% of females diagnosed with a mental disorder. The IRIS was an effective screener for substance use disorders, with high sensitivity of 94% and low specificity of 33%. The screener was less effective in identifying depression (sensitivity 82%, specificity 59%) and anxiety (sensitivity 68%, specificity 60%).DiscussionThe IRIS is the first culturally appropriate screening instrument to be validated for the risk of drug and alcohol and mental disorder among Indigenous adults in custody.ConclusionsThis study demonstrated that the IRIS is a valid tool for screening of alcohol and drug use risk among an incarcerated Indigenous population. The IRIS could offer an opportunity to improve the identification, treatment and health outcomes for incarcerated Indigenous adults. [Ober C, Dingle K, Clavarino A, Najman JM, Alati R, Heffernan EB. Validating a screening tool for mental health and substance use risk in an Indigenous prison population. Drug Alcohol Rev 2013;32:611–617]
Bitrophic toxicity of Cry1Ac to Cycloneda sanguinea, a predator in Brazilian cotton
Authors: Nakasu Erich Y.T. , Dias Simoni C. , Pires Carmen S.S. , Andow David A. , Paula Debora Pires , Togni Pedro Henrique B. , Macedo Tainã R. , Sujii Edison R. , de Sá Maria F.G. , Fontes Eliana M.G. 
Published: June 2013

AbstractInsect predators are exposed to the Cry1Ac toxin in Bt cotton fields through several pathways. In this study, we investigated the effects of activated Cry1Ac added to a diet on Cycloneda sanguinea (L.) (Coleoptera: Coccinellidae), which is one of the main predators of non‐target pests in Brazilian cotton. Direct bitrophic exposure of C. sanguinea to Cry1Ac was done by feeding beetles with Aphis gossypii (Glover) (Hemiptera: Aphidae) sprayed with 500 μg per ml Cry1Ac solution. Larval and pupal survival, development time, aphid consumption, and adult longevity were recorded daily. Couples within the same experimental treatment were paired and numbers of eggs laid and hatched per female were recorded daily. Net replacement rate was calculated for each female. During development, a C. sanguinea larva consumed on average 1.8 μg of activated Cry1Ac. No significant differences due to Cry1Ac were observed for any of the response variables, except aphid consumption. Larvae receiving Cry1Ac consumed more aphids than larvae receiving distilled water alone. Additional statistical analyses were conducted to evaluate independence of responses, and for the independent responses, a simple meta‐analysis was conducted to test the null hypothesis that all responses were zero. Nearly all of the response variables were statistically independent. Two pairs of responses were not independent, but the associated multivariate tests were not significant. The meta‐analysis suggested that all effects were not different from random variation around zero and no cumulative effects could be detected. Our results indicated that bitrophic exposure to activated Cry1Ac is likely to have little or no adverse ecological effect on C. sanguinea.
Dravet syndrome: New potential genetic modifiers, imaging abnormalities, and ictal findings
Authors: Gaily Eija , Anttonen Anna‐Kaisa , Valanne Leena , Liukkonen Elina , Träskelin Ann‐Liz , Polvi Anne , Lommi Markus , Muona Mikko , Eriksson Kai , Lehesjoki Anna‐Elina 
Published: June 2013

SummaryPurposeDravet syndrome is an autosomal dominant epileptic encephalopathy of childhood, which is caused mainly by SCN1A and PCHD19 mutations. Although Dravet syndrome is well recognized, the causes of acute encephalopathy are still elusive, and reported data on ictal electroencephalography (EEG) and structural brain abnormalities are scarce.MethodsWe studied 30 children who fulfilled the clinical criteria for Dravet syndrome. All patients were screened for SCN1A mutations and 25 for POLG mutations with bidirectional sequencing. Clinical data, including etiologic studies done as part of the clinical workup, were collected from hospital charts. Ictal video‐EEG recordings and magnetic resonance (MR) images were reanalyzed by the authors.Key FindingsSCN1A mutations were found in 25 patients (83%). Two SCN1A mutation–negative patients had chromosomal translocations involving chromosomes 9 and X, and one had a mutation in PCDH19. Prolonged seizures were associated with acute encephalopathy in three SCN1A mutation–positive patients. One showed evidence of a significant hypoxic–ischemic event during status epilepticus. The other two demonstrated new persistent neurologic deficits postictally; they both carried heterozygous POLG variants (p.Trp748Ser or p.Gly517Val). Hippocampal sclerosis or loss of gray–white matter definition in the temporal lobe was observed in 7 of 18 patients who had MRI after age 3 years (39%). Motor seizures were recorded on video‐EEG for 15 patients, of whom 12 were younger than 6 years at recording; 11 patients (73%) showed posterior onsets.SignificanceOur data imply that a heterozygous X;9 translocation and rare POLG variants may modify the clinical features of Dravet syndrome. The latter may increase susceptibility for acute encephalopathy. Temporal lobe abnormalities are common in patients imaged after 3 years of age. Focal seizures seem to localize predominantly in the posterior regions in young children with Dravet syndrome.
Keratinocyte cell lines derived from severe generalized recessive Epidermolysis Bullosa patients carrying a highly recurrent COL7A1 homozygous mutation: models to assess cell and gene therapies in vitro and in vivo
Authors: Chamorro Cristina , Almarza David , Duarte Blanca , Llames Sara G. , Murillas Rodolfo , García Marta , Cigudosa Juan C. , Espinosa‐Hevia Luis , Escámez Maria José , Mencía Ángeles , Meana Álvaro , García‐Escudero Ramón , Moro Rosa , Conti Claudio J. , Del Río Marcela , Larcher Fernando 
Published: June 2013

AbstractRecessive dystrophic epidermolysis bullosa (RDEB) is caused by deficiency of type VII collagen due to COL7A1 mutations such as c.6527insC, recurrently found in the Spanish RDEB population. Assessment of clonal correction–based therapeutic approaches for RDEB requires large expansions of cells, exceeding the replication capacity of human primary keratinocytes. Thus, immortalized RDEB cells with enhanced proliferative abilities would be valuable. Using either the SV40 large T antigen or papillomavirus HPV16‐derived E6‐E7 proteins, we immortalized and cloned RDEB keratinocytes carrying the c.6527insC mutation. Clones exhibited high proliferative and colony‐forming features. Cytogenetic analysis revealed important differences between T antigen‐driven and E6‐E7‐driven immortalization. Immortalized cells responded to differentiation stimuli and were competent for epidermal regeneration and recapitulation of the blistering RDEB phenotype in vivo. These features make these cell lines useful to test novel therapeutic approaches including those aimed at editing mutant COL7A1.
Time‐dependent multiaxial fatigue and life prediction for nickel‐based GH4169 alloy
Authors: Sun G. Q. , Wang D. , Shang D. G. 
Published: June 2013

ABSTRACTThe fatigue behaviour of nickel‐based GH4169 alloy was studied under multiaxial loading at 650 °C. During the middle and late stages of the fatigue life at 650 °C, the axial and shear maximum stresses continue to decrease and plastic strains continue to increase, while at 360 °C different phenomena are observed. The intergranular cracks and certain quantities oxygen were observed in the fracture surfaces. The damage of creep and oxidation are related to temperature and strain range. The life prediction results with a time‐dependent fatigue damage model show the time‐related factors have a certain influence on the fatigue damage.
The effect of frequency on secondary wave mode generation for ultrasonic health monitoring of fatigue damaged plate structures
Authors: Doherty C. , Chiu W. K. 
Published: June 2013

ABSTRACTA computational frequency analysis of secondary wave mode generation is considered in this paper. Secondary wave mode conversion at the tip of a through‐thickness slit in a circular isotropic plate is analysed similar to a classical Sommerfeld's diffraction problem. The analysis is useful for quantitative non‐destructive evaluation of fatigue cracked plate structures that involve propagation of a single wave mode (a vertically polarised symmetric Lamb mode) and measurement of the amplitude of a new mode (a horizontally polarised shear mode). A two‐dimensional finite element model is first introduced as a baseline reference for the non‐dispersive scenario. This model is used to study the effect that P‐wave angle of incidence has on the mode converted S‐wave from the tip of the slit. A dispersive three‐dimensional finite element model of the circular plate is then introduced at a number of frequency‐thickness products within the range 0.5–3 MHz.mm−1. Within this frequency–thickness range, significant changes to the through‐thickness displacement profiles of the incident S0 Lamb mode occur. The secondary SH0 plate wave mode generated by mode conversion from the 45o incident S0 Lamb mode at the tip of a slit is analysed to establish the efficiency of the mode conversion. Visualisations of the wave fields demonstrate that at frequency–thickness products higher than the first symmetric Lamé mode (where there is no in‐plane surface displacement), the in‐plane through‐thickness displacement profile of the incident S0 Lamb mode becomes incompatible with that of the SH0 plate mode, and the mode conversion phenomenon is thus severely compromised.
Prescription Headache Medication in OEF/OIF Veterans: Results From the Women Veterans Cohort Study
Authors: Seng Elizabeth K. , Driscoll Mary A. , Brandt Cynthia A. , Bathulapalli Harini , Goulet Joseph , Silliker Norman , Kerns Robert D. , Haskell Sally G. 
Published: June 2013

ObjectiveTo examine differences in male and female veterans of Operations Enduring Freedom/Iraqi Freedom (OEF/OIF) period of service in taking prescription headache medication, and associations between taking prescription headache medication and mental health status, psychiatric symptoms, and rates of traumatic events.BackgroundHeadaches are common among active service members and are associated with impairment in quality of life. Little is known about headaches in OEF/OIF veterans.MethodsVeterans participating in the Women Veterans Cohort Study responded to a cross‐sectional survey to assess taking prescription headache medication, mental health status (Post Deployment Health Assessment), psychiatric symptoms (portions of the Brief Patient Health Questionnaire and the Posttraumatic Stress Disorder Checklist), and traumatic events (the Traumatic Life Events Questionnaire and queries regarding military trauma). Gender differences among taking prescription headache medication, health status, psychiatric symptoms, and traumatic events were examined. Regression analyses were used to examine the influence of gender on the associations between taking prescription headache medication and health status, psychiatric symptoms, and traumatic events.Results139/551 (25.2%) participants reported taking prescription headache medication in the past year. A higher proportion of women veterans (29.1%) reported taking prescription medication for headache in the last year compared with men (19.7%). Taking prescription headache medication was associated with poorer perceived mental health status, higher anxiety and posttraumatic stress disorder symptoms, and higher rates of traumatic events. The association between prescription headache medication use and perceived mental health status, and with the association between prescription headache medication use and posttraumatic stress disorder symptoms, was stronger for men than for women.ConclusionsAmong OEF/OIF veterans, the prevalence of clinically relevant headache is high, particularly among women veterans. Taking prescription headache medication is associated with poor mental health status, higher rates of psychiatric symptoms, and higher rates of traumatic events; however, these variables did not appear to meaningfully account for gender differences in prevalence of taking prescription headache medication. Future research should endeavor to identify factors that might account for the observed differences.
Optimal Use of Acute Headache Medication: A Qualitative Examination of Behaviors and Barriers to Their Performance
Authors: Seng Elizabeth K. , Holroyd Kenneth A. 
Published: June 2013

ObjectiveThis study aims to qualitatively examine the behaviors required to optimally use acute headache medication and the barriers to successful performance of these behaviors.BackgroundThe efficacy of drug treatment is partly determined by medication adherence. The adherence literature has focused almost exclusively on the behaviors required to optimally use medications that are taken on a fixed schedule, as opposed to medications taken on an as needed basis to treat acute episodes of symptoms, such as headaches.MethodsTwenty‐one people with headache and 15 health care providers participated in qualitative phenomenological interviews that were transcribed and coded by a multidisciplinary research team using phenomenological analysis.ResultsInterviews revealed 8 behaviors required to optimally use acute headache medication, including cross‐episode behaviors that people with headache regularly perform to ensure optimal acute headache medication use, and episode‐specific behaviors used to treat an individual headache episode. Interviews further revealed 9 barriers that hinder successful performance of these behaviors.ConclusionsBehaviors required to optimally use acute headache medication were numerous, often embedded in a larger chain of behaviors, and were susceptible to disruption by numerous barriers.