bluuebunny/crossref_metadata_2025_split huggingface dataset:
A new implant with solid core and porous surface: The biocompatability with bone
Authors: Yang Xu , Wang Dihua , Liang Youde , Yin Huayi , Zhang Shuang , Jiang Tao , Wang Yining , Zhou Yi 
Published: August 2013

AbstractThis research investigated osteogenic potencies of Farthing–Fray–Chen Titanium (FFcTi) implant with transitional porous‐solid structure. The material characteristics, biomechanical property, osteogenic performances were assessed. FFcTi showed similar roughness as sand‐blasted and acid etched titanium (SA), but was more hydrophilic than SA and machined commercial pure titanium (MA). Young's modulus of FFcTi implant in compressive tests was 15.8 ± 6.3 GPa, which was close to bone. In vitro observations manifested excellent spreading abilities of MC3T3‐E1 cell on FFcTi and SA. Adhesion rates of MC3T3‐E1 cells at 4 h gradually decreased on MA, SA, and FFcTi surfaces (MA > SA, p < 0.01; SA > FFcTi, p < 0.05), while cell proliferation ability on FFcTi was weaker than MA during 1–6 days (p < 0.01) and similar to MA and SA in day 11. ALP activity of cells on FFcTi at 14 day was higher than MA and lower than SA (p < 0.01). In a bone defect model of rabbits, BIC and bone volum ratio within 50 μm were significantly higher for FFcTi than MA (BIC, p < 0.01; BT0.05, p < 0.05) while bone volume ratio within 100 and 500 μm were of no differences. Micro CT analysis also showed similar results to the histomorphometric data. Thus, we conclude that FFcTi with melting sphere based multiporous structure has a hydrophilic, rough surface, and close modulus to bone. In vitro, its low proliferation and ALP activity promotion were similar to other micro scale roughed surface. In vivo test showed better osteogenesis ability when compared with MA at least in 2 weeks. Thus, this Farthing–Fray–Chen Titanium implant seems to hold considerable potential for bone implant applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2395–2407, 2014.
Effects of SiO2, SrO, MgO, and ZnO dopants in tricalcium phosphates on osteoblastic Runx2 expression
Authors: Fielding Gary A. , Smoot Will , Bose Susmita 
Published: August 2013

AbstractCalcium phosphate materials share a compositional similarity to natural bone, which makes them excellent for use in orthopedic applications. Although these materials are osteoconductive, they lack strong osteoinductive capabilities and recent research has focused on the addition of biologics and pharmacologics with varying successes. In this study, trace elements that have been proven to play important roles in bone health and bone formation were incorporated into β‐tricalcium phosphate compacts in their oxide forms (SiO2, ZnO, SrO, and MgO). Cell material interactions were characterized using human fetal preosteoblastic cells. An MTT (3‐(4,5‐dimethylthiazol‐2‐yl)−2,5‐diphenyl tetrazolium bromide) assay was used to evaluate cellular proliferation. Cellular differentiation was evaluated using an enzymatic colorimetric alkaline phosphatase assay as well as immunohistochemistry for Runt‐related transcription factor 2 (Runx2) expression. Results prove ZnO and MgO to be effective mitogenic factors and SiO2, ZnO, and SrO to be capable of inducing rapid cellular differentiation. MgO was found to have little effect on the modulation of osteoblastic differentiation, likely due to more aggressive inherent cellular regulation of Mg2+. In addition to the results from the study, a signaling mechanism is proposed as to the action of the dopants for further consideration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2417–2426, 2014.
Oxygen‐rich coating promotes binding of proteins and endothelialization of polyethylene terephthalate polymers
Authors: Jaganjac Morana , Vesel Alenka , Milkovic Lidija , Recek Nina , Kolar Metod , Zarkovic Neven , Latiff Aishah , Kleinschek Karin‐Stana , Mozetic Miran 
Published: August 2013

AbstractThe formation of endothelial cell monolayer on prosthetic implants has not sufficiently explored. The main reasons leading to the development of thrombosis and/or intimal hyperplasia is the lack of endothelialization. In the present work, we have studied the influence of oxygen and fluorine plasma treatment of polyethylene terephthalate (PET) polymers on human microvascular endothelial cell adhesion and proliferation. We characterized the polymer surface, wettability, and oxidation potential upon plasma treatment. Moreover, binding of serum and media compounds on PET surface was monitored by Quartz crystal microbalance method, X‐ray photoelectron spectroscopy, and atomic force microscopy. Cell adhesion and morphology was assessed by light and scanning electron microscopy. The influence of plasma treatment on induction of cellular oxidative stress and cell proliferation was evaluated. The results obtained showed that treatment with oxygen plasma decreased the oxidation potential of the PET surface and revealed the highest affinity for binding of serum components. Accordingly, the cells reflected the best adhesion and morphological properties on oxygen‐treated PET polymers. Moreover, treatment with oxygen plasma did not induce intracellular reactive oxygen species production while it stimulated endothelial cell proliferation by 25% suggesting the possible use of oxygen plasma treatment to enhance endothelialization of synthetic vascular grafts. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2305–2314, 2014.
Vascular endothelial growth factor‐loaded injectable hydrogel enhances plasticity in the injured spinal cord
Authors: des Rieux Anne , De Berdt Pauline , Ansorena Eduardo , Ucakar Bernard , Damien Jacobs , Schakman Olivier , Audouard Emilie , Bouzin Caroline , Auhl Dietmar , Simón‐Yarza Teresa , Feron Olivier , Blanco‐Prieto Maria J. , Carmeliet Peter , Bailly Christian , Clotman Fréderic , Préat Véronique 
Published: August 2013

AbstractWe hypothesized that vascular endothelial growth factor (VEGF)‐containing hydrogels that gelify in situ after injection into a traumatized spinal cord, could stimulate spinal cord regeneration. Injectable hydrogels composed of 0.5% Pronova UPMVG MVG alginate, supplemented or not with fibrinogen, were used. The addition of fibrinogen to alginate had no effect on cell proliferation in vitro but supported neurite growth ex vivo. When injected into a rat spinal cord in a hemisection model, alginate supplemented with fibrinogen was well tolerated. The release of VEGF that was incorporated into the hydrogel was influenced by the VEGF formulation [encapsulated in microspheres or in nanoparticles or in solution (free)]. A combination of free VEGF and VEGF‐loaded nanoparticles was mixed with alginate:fibrinogen and injected into the lesion of the spinal cord. Four weeks post injection, angiogenesis and neurite growth were increased compared to hydrogel alone. The local delivery of VEGF by injectable alginate:fibrinogen‐based hydrogel induced some plasticity in the injured spinal cord involving fiber growth into the lesion site. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 2345–2355, 2014.
Glucocorticoids Antagonize RUNX2 During Osteoblast Differentiation in Cultures of ST2 Pluripotent Mesenchymal Cells
Authors: Koromila Theodora , Baniwal Sanjeev K. , Song Yae S. , Martin Anthony , Xiong Jian , Frenkel Baruch 
Published: August 2013

ABSTRACTThe efficacy of glucocorticoids (GCs) in treating a wide range of autoimmune and inflammatory conditions is blemished by severe side effects, including osteoporosis. The chief mechanism leading to GC‐induced osteoporosis is inhibition of bone formation, but the role of RUNX2, a master regulator of osteoblast differentiation and bone formation, has not been well studied. We assessed effects of the synthetic GC dexamethasone (dex) on transcription of RUNX2‐stimulated genes during the differentiation of mesenchymal pluripotent cells into osteoblasts. Dex inhibited a RUNX2 reporter gene and attenuated locus‐dependently RUNX2‐driven expression of several endogenous target genes. The anti‐RUNX2 activity of dex was not attributable to decreased RUNX2 expression, but rather to physical interaction between RUNX2 and the GC receptor (GR), demonstrated by co‐immunoprecipitation assays and co‐immunofluorescence imaging. Investigation of the RUNX2/GR interaction may lead to the development of bone‐sparing GC treatment modalities for the management of autoimmune and inflammatory diseases. J. Cell. Biochem. 115: 27–33, 2014. © 2013 Wiley Periodicals, Inc.
The F region and topside ionosphere response to a strong geomagnetic storm at Arecibo
Authors: Gong Yun , Zhou Qihou , Zhang Shao Dong , Aponte Néstor , Sulzer Michael , González Sixto A. 
Published: August 2013

AbstractWe analyze the data derived from the Arecibo incoherent scatter radar measurements to investigate the response of the F region and topside ionosphere to a strong geomagnetic storm that occurred during the period of 5–6 August 2011. The meridional wind was extremely enhanced at the early stage of the storm. The peak velocity reached approximately 300 m/s at an altitude of 340 km, which is seldom seen at the Arecibo latitude. During the storm, the vertical ion drift caused by the meridional wind was positively correlated with that caused by the electric field, which is opposite to the quiet time relationship. The disturbed vertical ion drifts resulted in large ionospheric perturbations in the F and topside regions. Several collapses were observed in hmF2 during the storm night. NmF2 rapidly increased after the storm and then decreased around midnight. At an altitude of 610 km, the concentration of H+ and O+, and the ratio of H+ over electron density all exhibited large variations. The ratio of H+ over electron density changed from less than 10% to more than 80% in a matter of 2 hours in the morning of 6 August. One explanation for such a behavior is that vertical transport dominates over charge exchange late at night due to the lower concentration of O+.
Periodic alternating nystagmus of peripheral vestibular origin
Authors: Kim Sung H. , Chung Woon‐Kyo , Kim Bo G. , Hwang Chi S. , Kim Mi J. , Lee Won‐Sang 
Published: August 2013

Objectives/HypothesisPeriodic alternating nystagmus (PAN) is most commonly found either in its congenital form or after cerebellar/pontomedullary lesions. However, we identified PAN in 10 patients with peripheral vestibular disorders and will try to describe their characteristics to aid in the differential diagnosis between peripheral and central etiologies.Study DesignObservation of a case series.MethodPeripheral vestibular lesions were confirmed by neurological examinations, vestibular function tests, and brain magnetic resonance imaging (MRIs). Eye movements of the patients were recorded using electronystagmography or video nystagmography for a minimum of 10 minutes to confirm the nystagmus change in direction.ResultsThe final diagnoses of the patients included Meniere's disease (n = 3), acute labyrinthitis (n = 4), sudden sensorineural hearing loss with vertigo (n = 2), and vestibular schwannoma (n = 1). Direction‐changing spontaneous horizontal nystagmus with quiescent intervals was observed in all patients under dark conditions. The nystagmus was suppressed by visual fixation; and the results of oculomotor tests were normal for saccadic and smooth pursuit eye movements and optokinetic nystagmus. All patients showed mild to complete canal paresis on a bithermal caloric test. PAN progressed into unidirectional nystagmus of the contra‐lesion side in all patients within 48 hours.ConclusionPAN can be observed in patients with peripheral vestibular disorders, but detecting PAN in this subpopulation is difficult because of its transitory nature. The absence of central symptoms and signs, the visual suppression of PAN, normal oculomotor tests, and transient persistence are important diagnostic clues for differentiating peripheral from central PAN.Level of Evidence4. Laryngoscope, 124:980–983, 2014
Basaloid squamous cell carcinoma of the oral cavity: An analysis of 92 cases
Authors: Fritsch Valerie A. , Gerry Daniel R. , Lentsch Eric J. 
Published: August 2013

Objectives/HypothesisBasaloid squamous cell carcinoma (BSCC) is considered a rare and possibly more aggressive squamous cell carcinoma (SCC) variant. Until now, a series of exclusively oral cavity BSCC patients has not been previously reported. We endeavored to compare BSCC and SCC of the oral cavity, focusing on epidemiologic factors and survival outcomes.Study DesignRetrospective analysis of population‐based data.MethodsWe compared epidemiologic factors, clinicopathologic data, and disease‐specific survivals (DSS) between 92 patients with oral cavity BSCC and 15,181 patients with SCC.ResultsHigh‐grade tumors and distant metastases were more common in the BSCC group (P ≤0.001). On multivariable analysis controlling for disease stage, BSCC patients had similar DSS to those with typical SCC (P = 0.231). Although there was a trend favoring definitive radiotherapy for BSCC, there were no significant differences in treatment approach between BSCC and SCC. The choice of treatment modality (radiation, surgery ± radiation) did not reveal a difference in DSS between the two tumor subtypes.ConclusionAnalysis of the largest oral cavity BSCC series to date demonstrates that BSCC of the oral cavity carries a comparable prognosis to conventional‐type oral SCC.Level of Evidence2b. Laryngoscope, 124:1573–1578, 2014
High‐Intensity Focused Ultrasound‐Induced Thermal Effect for Solid Polymer Materials
Authors: Liu Bo , Xia Hesheng , Fei Guoxia , Li Guo , Fan Wenru 
Published: August 2013

In this study, high‐intensity focused ultrasound (HIFU)‐induced thermal effects for solid polymer materials are investigated systematically. Infrared camera imaging is used to monitor temperature changes. The effects of polymer structure, HIFU power, and sample thickness on the thermal effect are studied. The results show that the HIFU‐induced heating effect is very quickly, and can be controlled spatially and temporally. More importantly, it is obviously different for different polymers. The main reason is attributed to the various inner friction behaviors of macromolecular chains. This fundamental study is helpful to understand the interaction mechanism between HIFU and polymer, when HIFU is used as a trigger for drug release, shape memory, etc.
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Polyelectrolytes with Tunable Charge Based on Polydehydroalanine: Synthesis and Solution Properties
Authors: Günther Ulrike , Sigolaeva Larisa V. , Pergushov Dmitry V. , Schacher Felix H. 
Published: August 2013

Poly(tert‐butoxycarbonylaminomethylacrylate) (PtBAMA), a derivative of polydehydroalanine (PDha), is synthesized using free radical polymerization (FRP) and nitroxide mediated polymerization (NMP). Due to the presence of orthogonal protective groups, the resulting polymers can be selectively deprotected to yield either a polyanion (poly(tert‐butoxycarbonylaminoacrylic acid), PtBAA) or a polycation (poly(aminomethylacrylate), PAMA). Deprotection of both the amino‐ and the carboxyl‐functionality in a sequential manner leads to the potential polyzwitterion polydehydroalanine (PDha). The pH‐dependent solution behavior of PtBAA, PAMA, and PDha are examined in aqueous solution by potentiometric and turbidimetric titrations as well as ζ‐potential measurements.
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Ab Initio Study of Proton Transfer and Interfacial Properties in Phosphoric Acid‐Doped Polybenzimidazole
Authors: Li Shuo , Fried J. R. 
Published: August 2013

AbstractQuantum mechanics calculations and hybrid ab initio/empirical molecular dynamics simulations using the ONIOM scheme have been used to investigate proton transfer and interfacial properties in phosphoric acid (PA)‐doped polybenzimidazole. Quantum mechanics calculations indicate that hydrogen bonding between PA and benzimidazole (with PA as the hydrogen donor) is the major interaction between PA and polybenzimidazole at low PA‐doping levels. Comparing energy barriers for different proton transfer pathways indicates that proton transfer is prone to occur between the same molecules or between a molecule and its corresponding ion. The atom‐centered density matrix propagation approach coupled with ONIOM using density functional theory and universal force field calculations indicates that protonation of the “=N–” atom on the imidazole ring occurs when there are two or more PA molecules surrounding each imidazole ring. These conclusions agree with the experimental results in the literature.
Probiotic VSL#3‐induced TGF‐β ameliorates food allergy inflammation in a mouse model of peanut sensitization through the induction of regulatory T cells in the gut mucosa
Authors: Barletta Bianca , Rossi Giacomo , Schiavi Elisa , Butteroni Cinzia , Corinti Silvia , Boirivant Monica , Di Felice Gabriella 
Published: August 2013

ScopeAmong food allergies, peanut allergy is frequently associated with severe anaphylactic reactions. In the need for safe and effective therapeutic strategies, probiotics may be considered on the basis of their immunomodulatory properties. The aim of the present study was to investigate the immunological mediators involved in the effects of probiotic VSL#3 oral supplementation on Th2 inflammation and anaphylaxis in a mouse model of peanut allergy.Methods and resultsVSL#3 supplementation to peanut‐sensitized mice was effective in ameliorating anaphylaxis and Th2‐mediated inflammation, by promoting regulatory responses in the jejunum mucosa and in the mesenteric lymph node, as evaluated by ELISA, real‐time PCR, histologic, and immunohistochemical analysis. Probiotic‐induced TGF‐β mediates its protective effects through the induction of regulatory T cells expressing FOXP3 and/or latency‐associated peptide, as proven by in vivo blockade of TGF‐β in VSL#3‐treated mice with a neutralizing monoclonal antibody one day before challenge.ConclusionTGF‐β, induced in the gut by VSL#3 supplementation, is capable of reducing the Th2 inflammation associated with food anaphylaxis in a mouse model of peanut sensitization. TGF‐β acts through the induction/maintenance of regulatory T cells expressing FOXP3 and/or latency‐associated peptide. Probiotics supplementation may represent an effective and safe strategy for treating food allergies in adult population.
Complexation of rhodium(II) tetracarboxylates with aliphatic diamines in solution: 1H and 13C NMR and DFT investigations
Authors: Jaźwiński Jarosław , Sadlej Agnieszka 
Published: August 2013

AbstractThe complexation of rhodium(II) tetraacetate, tetrakistrifluoroaceate and tetrakisoctanoate with a set of diamines (ethane‐1,diamine, propane‐1,3‐diamine and nonane‐1,9‐diamine) and their N,N′‐dimethyl and N,N,N′,N′‐tetramethyl derivatives in chloroform solution has been investigated by 1H and 13C NMR spectroscopy and density functional theory (DFT) modelling. A combination of two bifunctional reagents, diamines and rhodium(II) tetracarboxylates, yielded insoluble coordination polymers as main products of complexation and various adducts in the solution, being in equilibrium with insoluble material. All diamines initially formed the 2 : 1 (blue), (1 : 1)n oligomeric (red) and 1 : 2 (red) axial adducts in solution, depending on the reagents' molar ratio. Adducts of primary and secondary diamines decomposed in the presence of ligand excess, the former via unstable equatorial complexes. The complexation of secondary diamines slowed down the inversion at nitrogen atoms in NH(CH3) functional groups and resulted in the formation of nitrogenous stereogenic centres, detectable by NMR. Axial adducts of tertiary diamines appeared to be relatively stable. The presence of long aliphatic chains in molecules (adducts of nonane‐1,9‐diamines or rhodium(II) tetrakisoctanoate) increased adduct solubility. Hypothetical structures of the equatorial adduct of rhodium(II) tetraacetate with ethane‐1,2‐diamine and their NMR parameters were explored by means of DFT calculations. Copyright © 2013 John Wiley & Sons, Ltd.
Bayesian model aggregation for ensemble‐based estimates of protein pKavalues
Authors: Gosink Luke J. , Hogan Emilie A. , Pulsipher Trenton C. , Baker Nathan A. 
Published: August 2013

ABSTRACTThis article investigates an ensemble‐based technique called Bayesian Model Averaging (BMA) to improve the performance of protein amino acid pKapredictions. Structure‐based pKacalculations play an important role in the mechanistic interpretation of protein structure and are also used to determine a wide range of protein properties. A diverse set of methods currently exist for pKaprediction, ranging from empirical statistical models to ab initio quantum mechanical approaches. However, each of these methods are based on a set of conceptual assumptions that can effect a model's accuracy and generalizability for pKaprediction in complicated biomolecular systems. We use BMA to combine eleven diverse prediction methods that each estimate pKavalues of amino acids in staphylococcal nuclease. These methods are based on work conducted for the pKaCooperative and the pKameasurements are based on experimental work conducted by the García‐Moreno lab. Our cross‐validation study demonstrates that the aggregated estimate obtained from BMA outperforms all individual prediction methods with improvements ranging from 45 to 73% over other method classes. This study also compares BMA's predictive performance to other ensemble‐based techniques and demonstrates that BMA can outperform these approaches with improvements ranging from 27 to 60%. This work illustrates a new possible mechanism for improving the accuracy of pKaprediction and lays the foundation for future work on aggregate models that balance computational cost with prediction accuracy. Proteins 2014; 82:354–363. © 2013 Wiley Periodicals, Inc.
First‐principles study of structural, elastic, and electronic properties of M23C6 and MC carbides (M = Ru, Rh, Pd, Os, Ir, and Pt)
Authors: Medvedeva N. I. , Ivanovskii A. L. 
Published: August 2013

AbstractUsing first‐principles calculations within the density functional theory we studied the electronic structure, elastic properties, and stability of M23C6 carbides, where M are the platinum‐metals: Ru, Rh, Pd, Os, Ir, or Pt. The lattice constants, elastic parameters, formation energies, and densities of states of M23C6 were compared with those for mono‐carbides MC. We demonstrated that these carbides have the positive formation energies and predicted the mechanically stable phases. We found that M23C6 carbides are energetically more favorable than the corresponding MC carbides due to the stronger M–M interactions in M23C6. Mechanically stable and unstable M23C6 and MC carbides.
Heat, molecular vibrations, and adiabatic driving in non‐equilibrium transport through interacting quantum dots
Authors: Haupt F. , Leijnse M. , Calvo H. L. , Classen L. , Splettstoesser J. , Wegewijs M. R. 
Published: August 2013

AbstractIn this article we review aspects of charge and heat transport in interacting quantum dots and molecular junctions under stationary and time‐dependent non‐equilibrium conditions due to finite electrical and thermal bias. In particular, we discuss how a discrete level spectrum can be beneficial for thermoelectric applications, and investigate the detrimental effects of molecular vibrations on the efficiency of a molecular quantum dot as an energy converter. In addition, we consider the effects of a slow time‐dependent modulation of applied voltages on the transport properties of a quantum dot and show how this can be used as a spectroscopic tool complementary to standard dc‐measurements. Finally, we combine time‐dependent driving with thermoelectrics in a double‐quantum dot system –a nanoscale analog of a cyclic heat engine –and discuss its operation and the main limitations to its performance.