bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous quantification of icaritin and its novel 3‐methylcarbamate prodrug in rat plasma using HPLC–MS/MS and its application to pharmacokinetic study
Authors: Li Fengxiao , Wang Weiping , Zhai Yixiu , Fan Jiaqi , Jiang Qikun , Zhang Tianhong 
Published: August 2024

AbstractA sensitive, rapid, and simple HPLC–MS/MS method was first developed and fully validated to determine the icaritin (ICT) and its novel 3‐methylcarbamate prodrug (3N) simultaneously in rat plasma. Analytes were extracted from rat plasma using a liquid–liquid extraction (LLE) method. Chromatographic separation was performed on ACE Excel 2 C18‐Amide column. Quantitation of analytes was conducted on an LCMS‐8060 triple‐quadrupole tandem mass spectrometer. The quantitation mode was the multiple reaction monitoring via positive electrospray ionization. The calibration curve was linear over the concentration range of 1 to 200 ng/ml for ICT with a correlation coefficient of r = 0.9950 and 1 to 400 ng/ml for 3N with a correlation coefficient of r = 0.9956. The intra‐precision RSDs were ≤12% for ICT and 3N. The inter‐day precision RSDs were ≤10% for ICT and 3N. The accuracy RE was between −2.6% and 7.8% for ICT and 3N. The average ICT, 3N and IS recoveries were 87.9%, 83.6%, and 84.3%. The plasma matrix of ICT and 3N complied with the guidelines. ICT and 3N were stable in rat plasma under various tested conditions. This work has been successfully applied to studying the pharmacokinetics of ICT and 3N.
Influence of genetic co‐mutation on chemotherapeutic outcome in NPM1‐mutated and FLT3‐ITD wild‐type AML patients
Authors: Wu Quan , Zhang Yujiao , Yuan Baoyi , Huang Yun , Jiang Ling , Liu Fang , Yan Ping , Cheng Jiaying , Long Zhiquan , Jiang Xuejie 
Published: August 2024

AbstractBackgroundNucleophosmin 1 (NPM1) gene‐mutated acute myeloid leukemia (NPM1mut AML) is classified as a subtype with a favorable prognosis. However, some patients fail to achieve a complete remission or relapse after intensified chemotherapy. Genetic abnormalities in concomitant mutations contribute to heterogeneous prognosis of NPM1mut AML patients.MethodsIn this study, 91 NPM1‐mutated and FLT3‐ITD wild‐type (NPM1mut/FLT3‐ITDwt) AML patients with intermediate‐risk karyotype were enrolled to analyze the impact of common genetic co‐mutations on chemotherapeutic outcome.ResultsOur data revealed that TET1/2 (52/91, 57.1%) was the most prevalent co‐mutation in NPM1mut AML patients, followed by IDH1/2 (36/91, 39.6%), DNMT3A (35/91, 38.5%), myelodysplastic syndrome related genes (MDS‐related genes) (ASXL1, BCOR, EZH2, RUNX1, SF3B1, SRSF2, STAG2, U2AF1 and ZRSR2 genes) (35/91, 38.5%), FLT3‐TKD (27/91, 29.7%) and GATA2 (13/91, 14.3%) mutations. Patients with TET1/2mut exhibited significantly worse relapse‐free survival (RFS) (median, 28.7 vs. not reached (NR) months; p = 0.0382) compared to patients with TET1/2wt, while no significant difference was observed in overall survival (OS) (median, NR vs. NR; p = 0.3035). GATA2mut subtype was associated with inferior OS (median, 28 vs. NR months; p < 0.0010) and RFS (median, 24 vs. NR months; p = 0.0224) compared to GATA2wt. By multivariate analysis, GATA2mut and MDS‐related genesmut were independently associated with worse survival.ConclusionMutations in TET1/2, GATA2 and MDS‐related genes were found to significantly influence the chemotherapeutic outcome of patients with NPM1mut AML. The findings of our study have significant clinical implications for identifying patients who have an adverse response to frontline chemotherapy and provide a novel reference for further prognostic stratification of NPM1mut/FLT3‐ITDwt AML patients.
Production of a functional snack from germinated brown rice fermented with baobab pulp (Adansonia digitata L.) as a starter
Authors: Adedeji Olajide Emmanuel , Nathan Blessing Fxafa , Sunday Godiya Ene 
Published: August 2024

AbstractBackground and ObjectivesBrown rice (BR) is becoming popular in functional food product development. To improve its utilization, it is necessary to subject BR to bioprocessing techniques such as germination. The objective of this study was to evaluate the quality of snacks developed from different rice types fermented with baobab pulp powder (BPP) as a starter.FindingsThe germinated BR (GBR) snack had higher protein, ash, soluble dietary fiber, resistant starch, total phenolic, carotenoid contents, and antioxidant activities than snacks from white rice (WR) and BR. The textural and sensory attributes of the snacks from GBR and WR were similar. The WR snack had a higher rapidly digestible starch and lower slowly digestible starch compared to the GBR snack. The inclusion of BPP increased protein, ash, riboflavin, dietary fiber, resistant starch, carotenoid contents, and antioxidant activities of all the samples.ConclusionsGBR and BPP could serve as invaluable ingredients in producing nutritious and healthy snacks.Significance and NoveltyDue to its health‐promoting qualities, utilizing GBR in product development is increasing. However, studies adopting GBR in traditional rice‐based products are limited; hence, the focus of this study.
Predicting fumonisins in Iowa corn: Gradient boosting machine learning
Authors: Branstad‐Spates Emily , Castano‐Duque Lina , Mosher Gretchen , Hurburgh Charles , Rajasekaran Kanniah , Owens Phillip , Edwin Winzeler H. , Bowers Erin 
Published: August 2024

AbstractBackground and ObjectivesFumonisin (FUM), a secondary metabolite from Fusarium spp., poses major concerns for the United States corn industry. This study evaluated a prepublished Illinois‐centric predictive model with historical Iowa FUM contamination data using gradient boosting machine (GBM) learning and compared influential predictors with an Iowa‐centric model. Corn samples (n = 529) were collected from 2010, 2020, and 2021 in Iowa's 99 counties, and 2011 data were used for independent validation (n = 89).FindingsApplying a 2 ppm (mg/kg) threshold for FUM high and low contamination events, the overall accuracy was 71.08% and 85.39% for the Illinois‐ and Iowa‐centric models in 2011. Balanced accuracies were 60.23% and 50.00% for the Illinois‐ and Iowa‐centric models. For Iowa's remaining years (testing data), the overall accuracy was 98.10%, and balanced accuracy was 50.00%.ConclusionsFUM‐GBM analyses determined the top influential predictor for the Illinois‐centric model was satellite‐acquired normalized difference vegetation index (NDVI) (Veg_index) in March, whereas the top predictor for the Iowa‐centric model was precipitation (PRCP) in October.Significance and NoveltyResults indicate that meteorological and agronomic events, such as PRCP and Veg_index in early planting stages and during harvest, may influence the probability of high FUM levels in corn.
New‐onset sarcoidosis in a patient with long COVID
Authors: Rodriguez‐Nava Guillermo , El Kamari Vanessa , Chang Harvey , Egoryan Goar , Bonilla Hector F. 
Published: August 2024

Key Clinical MessageLong COVID, often following SARS‐CoV‐2 infection, may stem from sustained inflammation, overlapping with autoimmune diseases like sarcoidosis. Though specific treatments lack, this link could shape future diagnostic and therapeutic methods.
Emphysematous pyelonephritis diagnosed by acute changes detected via computed tomography: A case report
Authors: Kato Yoichiro , Ishii Shuhei , Goto Yuta , Nozaki Yasushi , Kawamura Tatsuya , Morino Gota , Hoshi Shintaro , Takahashi Gaku , Obara Wataru 
Published: August 2024

Key Clinical MessageThis is the first case report in which computed tomography (CT) images of a patient with emphysematous pyelonephritis (EPN) capture the time course of emphysema from onset to resolution through conservative treatment. To re‐evaluate EPN, including refractory urinary tract infections, CT scans after 72 h might be helpful.
Long‐term rehabilitation with interferential current stimulation for persistent dysphagia in anti‐N‐methyl‐d‐aspartate receptor encephalitis: A case report
Authors: Sugahara Takahiro , Nagami Shinsuke , Sato Ryota , Ishizaki Naohiko , Fujishima Ichiro , Aikawa Fumihito 
Published: August 2024

Key Clinical MessageAnti‐N‐methyl‐d‐aspartate receptor encephalitis is an autoimmune disorder characterized by various neurological symptoms with a relatively favorable prognosis. We present a case of prolonged dysphagia successfully managed with outpatient rehabilitation, including interferential current stimulation and resistance exercises. Significant improvement was observed, highlighting the efficacy of combined treatment in overcoming chronic dysphagia.
Googling and health anxiety in older age—A case study of CBT incorporating a single case experimental design
Authors: Hill Sean , Watts Daniel 
Published: August 2024

Key Clinical MessageThe use of online search engines to “google” health‐related information is common in health anxiety, and requires careful consideration within psychological treatment. Its nature in older adult populations is less closely understood. This report demonstrates the reduction in googling frequency using cognitive‐behavioral therapy in an 83‐year‐old female with health anxiety.
Cu and Fe modified Cerium and Zirconium Oxides for the Oxidation of Cyclohexane to KA Oil
Authors: Vomeri Alessandro , Stucchi Marta , Villa Alberto , Hungría Ana B. , Calvino José , Prati Laura 
Published: August 2024

AbstractCe and Zr oxides have been tested in the oxidation of cyclohexane to KA oil because of the possibility to tune their crystal structure and redox properties by introducing defects, and of modifying their mechanical and thermal stability. The redox properties of the two oxides, prepared by the inverse microemulsion technique, have been tuned by homogeneously including Cu or Fe (10 % molar) into their structures. These modifications led to very different behaviours in terms of both KA oil productivity and K/A ratios, especially upon undergoing reduction treatment, being the reduced Cu‐modified CeO2 oxide the most active catalyst, able to produce 2.2 mmol of KA oil after 5 hours with 93.2 % selectivity (K/A 2.6). The materials obtained have been characterized by solid state analytical techniques to study their structure, morphologies as well as their bulk and surface chemistry (STEM/EDX, BET, XRD, XPS).
A Novel 3DOM‐ZrO2/Cd0.5Zn0.5S Catalyst with Excellent Photocatalytic H2 Evolution Performance
Authors: Liu Guozhong , Liu Haizhen , Hu Ziying , Wang Shanhao , Jia Hongpeng , Chen Jing 
Published: August 2024

AbstractThree‐dimensional ordered macroporous (3DOM) materials have good properties in the mass transfer and diffusion. Herein, a novel Z‐scheme 3DOM‐ZrO2/Cd0.5Zn0.5S (Zr3D/CZS) heterojunction catalyst was prepared and applied to photocatalytic water splitting for hydrogen production. The results show that the three‐dimensional macroporous zirconia is conducive to the transport of photogenerated charges, and the optimal catalyst 2Zr3D/CZS achieves the highest hydrogen production rate of 83.12 mmol g−1 h−1 under the full spectrum illumination, which is 213 and 2 times higher than that of pure ZrO2 (0.39 mmol g−1 h−1) and CZS (40.86 mmol g−1 h−1), respectively. Under 450 nm light irradiation, the apparent quantum efficient is up to 44 %. In addition, the catalyst also showed good stability. The possible charge transfer mechanism was proposed under the full spectrum and visible light, respectively. This work provides an efficient strategy for design and synthesis of high active ZrO2 based photocatlysts for hydrogen production.
Assessing application errors on sports fields across varying levels of sprayer technology and operator experience
Authors: Floyd Weston W. , Muesse Megan R. , Tucker Hailey N. , Alabi Opeyemi E. , Winger Jacob O. , Straw Chase M. 
Published: August 2024

AbstractThis study aimed to quantify misses, overlaps, and oversprays on sports fields using different sprayer technologies across operator experience levels. Conducted from Winter 2022 to Summer 2023 at Veterans Park and Southwood Park in College Station, TX, identical studies were conducted on softball, baseball, and soccer fields. Employing a crossover design, six treatments were administered to three fields at each location, using manual, Global Navigation Satellite System (GNSS), and GNSS + autosteer sprayer technologies. Operators ranged from a highly experienced former sports field manager with nearly 20 years of experience to four inexperienced undergraduate students. Field boundaries were georeferenced for target area determination and treatment applications, using water and a spray volume of 65 gal per acre. ArcMap calculated percentage target area missed, overlapped, and oversprayed based on the actual versus intended volume sprayed. Data were subjected to analysis of variance, and means were separated using Fisher's protected LSD (α = 0.05). Applications by inexperienced operators using manual spray mode typically resulted in the highest rates of percentage target area missed and overlapped across locations and field types. The use of GNSS and autosteer technologies markedly reduced these errors and, consequently, percentage target area oversprayed. This enhancement in the consistency of applications led to a reduction of up to 4.6% in the total volume applied on the softball, baseball, and soccer fields. Therefore, the findings suggest that investing in GNSS‐equipped sprayers with autosteer not only decreases the dependency on experienced operators but also minimizes errors and reduces total volume applied.
Family Therapy for Kosovar Mothers Who Experienced Conflict‐Related Sexual Violence and Their Children in Postwar Times: A Pilot Randomised Waitlist‐Controlled Trial
Authors: Seery Christina , Rushiti Feride , Krasniqi Sebahate Pacolli , Çarkaxhiu Selvi Izeti , Xhemaili Vjosa Devaja , Limani Dafina Arifaj , Prapashtica Qëndresa , Turjaka Vesa , Zekaj Nebahate , Salihu Mimoza , Hjort Line , Ryan Joanne , Kaas‐Petersen Sara Helene , Wang Shr‐Jie Sharlenna 
Published: August 2024

ABSTRACTWomen who have experienced conflict‐related sexual violence report significant long‐term effects, including posttraumatic stress disorder (PTSD), depression and relationship difficulties. Research has demonstrated that maternal trauma is associated with children's behavioural difficulties and challenges in family functioning, such as impaired communication and harsh parenting. This pilot study is aimed at evaluating the preliminary effectiveness of family therapy for Kosovar mothers who experienced conflict‐related sexual violence in 1998–1999 and later developed PTSD and their children in improving family functioning and reducing behavioural difficulties in postwar times. Sixty‐four mothers were randomised to an intervention group or a waitlist control group. Data was collected during a screening phase, at baseline before intervention initiation, after the intervention group completed family therapy and once the waitlist control group received the intervention. Generalised linear mixed models were used to analyse group differences in family functioning and children's behaviours over time. At follow‐up, mothers in the intervention group reported improved family functioning. However, mothers in the waitlist control group reported significantly fewer behavioural difficulties than mothers in the intervention group before the control group had started family therapy. There was no significant interaction between group condition and time for child‐rated family functioning. Overall, this pilot study suggests that family therapy could be effective in reducing the effects of intergenerational trauma related to PTSD and conflict‐related sexual violence. Future research should evaluate the long‐term effects of family therapy to assess if immediate effects were maintained.
Setting Up the JBrowse 2 Genome Browser
Authors: Diesh Colin , Buels Robert , Stevens Garrett , Bridge Caroline , Cain Scott , Stein Lincoln , Holmes Ian 
Published: August 2024

AbstractJBrowse 2 is a modular genome browser that can visualize many common genomic file formats. While JBrowse 2 supports a variety of different usages, it is particularly suited for deployment on websites, such as model organism databases or other web‐based genomic data resources. This protocol provides detailed instructions for setting up JBrowse 2 on an Ubuntu Linux web server, loading a reference genome from a FASTA format file, and adding a gene annotation track from a GFF3 format file. By the end of the protocol, users will have a working JBrowse 2 instance that is accessible via the web. © 2024 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol: Setting up JBrowse 2 on your web server
QTL mapping of preharvest sprouting resistance in bread wheat population SHA3/CBRD by Naxos confirms a negative effect of the 1BL.1RS translocation on falling number
Authors: Begum Most Champa , Belova Tatiana , Dieseth Jon Arne , Yang Ennian , Mosleth Ellen Færgestad , Uhlen Anne Kjersti , Lillemo Morten 
Published: August 2024

AbstractPreharvest sprouting (PHS) is a serious problem in wheat (Triticum aestivum L.) production areas with rainy harvest conditions. To explore the genetic control of PHS resistance, a population of 166 recombinant inbred lines from a cross between SHA3/CBRD and Naxos was evaluated in a total of eight field trials in Norway and China. PHS resistance was evaluated with both germination index (GI) and falling number (FN). A total of 15 PHS‐related quantitative trait loci (QTLs) were detected at chromosome arms 1RS, 2DL, 3BL, 4AL, 4BS, and 7BL. At 2DL and 3BL, QTLs for both GI and FN were detected in proximity to each other with PHS resistance from SHA3/CBRD, indicating a common genetic control of the two traits at these loci. At 4AL, a significant QTL for GI was detected with seed dormancy contributed by Naxos. Moreover, the Rht‐B1b dwarfing allele contributed by SHA3/CBRD showed a positive association with FN. On the other hand, QFN.nmbu‐1RS, carried by the 1BL.1RS translocation in SHA3/CBRD showed consistent negative effects on FN across all field trials where FN was measured. However, it did not have any notable effect on GI. The negative effect of QFN.nmbu‐1RS on FN was validated on a set of 113 wheat cultivars and breeding lines, where lines carrying the 1BL.1RS translocation showed significantly lower FN in 12 out of 14 independent field trials. The QTL and associated markers identified in this study will aid the breeding of more PHS‐resistant wheat cultivars.
A compact sub‐6 GHz band filtering patch antennae using step impedance resonators
Authors: Gnanamani Christina , Thomas Anita Jones Mary 
Published: August 2024

SummaryThis research study proposes a combined filter‐antenna design by employing stepped impedance resonator (SIR) techniques. The filtering antenna is designed around the unlicensed frequency band of 5.3 and 5.6 GHz. The filtenna realizes an insertion loss of −3.15 dB and return loss of −22 dB in the frequency band of 5.3 GHz and further −17.8 dB return loss in the 5.6‐GHz band. The developed filtenna achieves omni‐directional radiation pattern suitable for unlicensed applications with better selectivity and also results in low reflection loss. The observed voltage standing wave ratio is 1.6, and antenna gain is approximately 6.2 dBi in the 5.6‐GHz band. The simulation results obtained align consistently with real‐time measurements.
Functional measures and AD biomarkers among Hispanic and White non‐Hispanic older adults
Authors: Rodriguez Miriam J. , Mendoza Lisandra , Garcia Patricia , Duarte Andres , Padron Dilianna , Marsiske Michael , Fiala Jacob , Gonzalez Joanna , Duara Ranjan 
Published: August 2024

AbstractINTRODUCTIONPoorer baseline functioning is associated with long‐term cognitive decline among Hispanic older adults, but little is known about associations of these factors with Alzheimer's disease (AD) neuroimaging biomarkers.METHODSA total of 461 Hispanic and White non‐Hispanic (NHW) older adults who are cognitively normal (n = 76), had impaired cognition without mild cognitive impairment (MCI) (n = 41), or carried a diagnosis of MCI (n = 253) or dementia (n = 91) completed neuropsychological and functional assessment, genetic testing, and brain magnetic resonance imaging (MRI). Structural equation modeling (SEM) was used to examine predictive associations between functional and cognitive measures of AD neuroimaging biomarkers.RESULTSMRI volumes significantly predicted functional limitations in both groups. Sex and amyloid load significantly predicted functional limitations among the Hispanic group only. Years of education and MRI regional volume were the strongest predictors of cognition among both groups.DISCUSSIONResults indicate that functional performance is associated with early AD biomarkers among Hispanic older adults. Clinical implications are discussed.Highlights
The current study addresses health disparities in Alzheimer's disease (AD) and related dementia assessment among Hispanics by identifying measures sensitive to early AD biomarkers.
Associations of functional measures with AD genetic and neuroimaging biomarkers revealed that similarities in these associations exist between Hispanic and White non‐Hispanic individuals, but biological sex and amyloid load significantly predicted functional limitations among the Hispanic group only.
These results have clinical implications for physicians who treat Hispanic AD patients and indicate that when compared to traditional diagnostic assessments, functional assessments may better aid in AD diagnostic precision among Hispanics.
Lactate threshold evaluation in swimming using a sweat lactate sensor: A prospective study
Authors: Okawara Hiroki , Sawada Tomonori , Nakashima Daisuke , Fujitsuka Haruki , Muramoto Yuki , Hinokuma Daigo , Oshikiri Yuta , Ishizaki Keisuke , Miki Jiro , Hara Reira , Sano Motoaki , Sato Kazuki , Nakamura Masaya , Nagura Takeo , Katsumata Yoshinori 
Published: August 2024

AbstractSince assessing aerobic capacity is key to enhancing swimming performance, a simple and widely applicable technology should be developed. Therefore, we aimed to noninvasively visualize real‐time changes in sweat lactate (sLA) levels during swimming and investigate the relationship between lactate thresholds in sweat (sLT) and blood (bLT). This prospective study included 24 university swimmers (age: 20.7 s ± 1.8 years, 58% male) who underwent exercise tests at incremental speeds with or without breaks in a swimming flume to measure heart rate (HR), bLT, and sLT based on sLA levels using a waterproof wearable lactate sensor attached to the dorsal upper arm on two different days. The correlation coefficient and Bland–Altman methods were used to verify the similarities of the sLT with bLT and personal performance. In all tests, dynamic changes in sLA levels were continuously measured and projected onto the wearable device without delay, artifacts, or contamination. Following an initial minimal current response, with increasing speed the sLA levels increased substantially, coinciding with a continuous rise in HR. The speed at sLT strongly correlated with that at bLT (p < 0.01 and r = 0.824). The Bland–Altman plot showed a strong agreement (mean difference: 0.08 ± 0.1 m/s). This prospective study achieved real‐time sLA monitoring during swimming, even with vigorous movement. The sLT closely approximated bLT; both were subsequently validated for their relevance to performance.