bluuebunny/crossref_metadata_2025_split huggingface dataset:
Who are the independent editors, how did they reach their role and what are their associated job satisfactions?
Authors: BAVERSTOCK Alison , BLACKBURN Robert , ISKANDAROVA Marfuga 
Published: December 2014

ABSTRACTThis paper presents new evidence on the world of the independent editor in the publishing industry, analysing their demographic background, education and training, working practices, links with professional organizations, motivations for ‘going it alone‘, and job satisfaction. The results help contribute to the understanding of this important and growing segment of the publishing industry, challenge some of the commonly held beliefs about their role in the industry and set these within the broader context of changes in work organization in publishing. Contrary to the assumption that self‐publishing largely takes place without the involvement of an editor, research into the processes of self‐publishing (previously published in this journal) found that editors (and other providers of publishing services) were in fact often involved. The results are significant for the traditional publishing industry, the users of independent editorial services, and the editors themselves.
Evolution of the Transfer Code of Practice
Authors: PHILLPOTTS James , DEVENPORT Tim , MITCHELL Alison 
Published: December 2014

ABSTRACTThe Transfer Code of Practice was launched in 2008 as a set of best‐practice guidelines to be followed when a journal changes publisher, with the aim of ensuring uninterrupted access to content for subscribers. The Code has now been updated to reflect the continued evolution of the academic publishing process driven by new technologies, policies, and publishing practices.
Modelling fishers' response to discard prevention strategies: the case of the North Sea saithe fishery
Authors: Simons Sarah Laura , Döring Ralf , Temming Axel 
Published: December 2014

Abstract
Designing effective management plans requires understanding fishers' behaviour under that plan, because fishers change their behaviour in response to economic and management incentives, which in turn will lead to different fishery outcomes. This study presents a modelling framework for management strategy evaluations which takes into account the response of fishers to management schemes. Based on the upcoming discard ban, two discard prevention strategies were tested for the North Sea saithe fishery, where fleet segments have either no or a generally low quota for cod. Costs and benefits were assessed under the current management, a non-flexible system, where fleet segments had to stop fishing once the cod quota was reached and a flexible system where quota of saithe could be used to cover over-quota catch of cod at a ratio 1:5. The flexible scenario was beneficial both in protecting the North Sea saithe and cod stock and in increasing net profits of fleet segments in the long term. The avoidance behaviour of fleet segments to over-quota catch led to a high SSB level of saithe and cod in the long term, ensuring high long-term catches and profits. A non-flexible scenario had a negative impact on the saithe stock, because mainly juvenile saithe before spawning were caught reducing the spawning-stock biomass in the longer term. A non-flexible scenario was costly in terms of up to 29% lower net profits for individual fleet segments generating little economic incentive to be compliant.
Galactic hail: the origin of the high-velocity cloud complex C
Authors: Fraternali F. , Marasco A. , Armillotta L. , Marinacci F. 
Published: December 2014

Abstract
High-velocity clouds consist of cold gas that appears to be raining down from the halo to the disc of the Milky Way. Over the past 50 years, two competing scenarios have attributed their origin either to gas accretion from outside the Galaxy or to circulation of gas from the Galactic disc powered by supernova feedback (galactic fountain). Here, we show that both mechanisms are simultaneously at work. We use a new galactic fountain model combined with high-resolution hydrodynamical simulations. We focus on the prototypical cloud complex C and show that it was produced by an explosion that occurred in the Cygnus-Outer spiral arm about 150 Myr ago. The ejected material has triggered the condensation of a large portion of the circumgalactic medium and caused its subsequent accretion on to the disc. This fountain-driven cooling of the lower Galactic corona provides the low-metallicity gas required by chemical evolution models of the Milky Way's disc.
Power Cycles of Generation III and III+ Nuclear Power Plants
Authors: Dragunov Alexey , Saltanov Eugene , Pioro Igor , Kirillov Pavel , Duffey Romney 
Published: December 2014

It is well known that electrical power generation is the key factor for advances in industry, agriculture, and standard of living. In general, electrical energy can be generated by (1) nonrenewable energy sources such as coal, natural gas, oil, and nuclear; and (2) renewable energy sources such as hydro, wind, solar, biomass, geothermal, and marine. However, the main sources for electrical energy generation are (1) thermal—primarily coal and secondary natural gas, (2) “large” hydro, and (3) nuclear. Other energy sources might have a level of impact in some countries. Modern advanced thermal power plants have reached very high thermal efficiencies (55–62%). In spite of that, they are still the largest emitters of carbon dioxide into the atmosphere. Therefore, reliable non–fossil fuel energy generation, such as nuclear power, is becoming more and more attractive. However, current nuclear power plants (NPPs) are way behind in thermal efficiency (30–42%) compared to the efficiency of advanced thermal power plants. Therefore, it is important to consider various ways to enhance the thermal efficiency of NPPs. This paper presents a comparison of thermodynamic cycles and layouts of modern NPPs and discusses ways to improve their thermal efficiencies.
Schistosoma mansoni Soluble Egg Antigens Enhance T Cell Responses to a Newly Identified HIV-1 Gag H-2bEpitope
Authors: Bui Cac T. , Shollenberger Lisa M. , Paterson Yvonne , Harn Donald A. 
Published: December 2014

ABSTRACTSchistosome infection induces significant T helper type 2 (Th2) and anti-inflammatory immune responses and has been shown to negatively impact vaccine efficacy. Our goal was to determine if the administration of schistosome soluble egg antigens (SEA) would negatively influence the induction of cytotoxic T lymphocyte (CTL) and Th1-type T cell responses to an HIV candidate vaccine in the Th1-biased C57BL/6 mouse strain. Initial experiments failed, as we were unable to detect any response to the defined class I epitope for HIV-1 IIIB Gag. Therefore, we initiated an epitope mapping study to identify C57BL/6 (H-2b) T cell epitopes in HIV-1 IIIB Gag in order to perform the experiments. This analysis defined two previously unreported minimal class I H-2band class II I-Abepitopes for HIV-1 IIIB Gag. The newly defined HIV-1 IIIB Gag epitopes were used to evaluate the influence of SEA on the generation of CTL and Th1-type HIV-1 IIIB Gag responses. Surprisingly, in contrast to our hypothesis, we observed that the coadministration of SEA with aListeria monocytogenesvector expressing HIV-1 IIIB Gag (Lm-Gag) led to a significantly increased frequency of gamma interferon (IFN-γ)-producing CD8+and CD4+T cells in C57BL/6 mice compared to mice immunized with Lm-Gag only. These observations suggest that SEA contains, in addition to Th2-type and immune-suppressive molecules, substances that can act with the Lm-Gag vaccine to increase CTL and Th1-type vaccine-specific immune responses.
Evaluation of a Vaccine Formulation against Streptococcus pneumoniae Based on Choline-Binding Proteins
Authors: Miyaji Eliane N. , Vadesilho Cintia F. M. , Oliveira Maria Leonor S. , Zelanis André , Briles David E. , Ho Paulo L. 
Published: December 2014

ABSTRACTStreptococcus pneumoniaehas proteins that are attached to its surface by binding to phosphorylcholine of teichoic and lipoteichoic acids. These proteins are known as choline-binding proteins (CBPs). CBPs are an interesting alternative for the development of a cost-effective vaccine, and PspA (pneumococcal surface protein A) is believed to be the most important protective component among the different CBPs. We sought to use CBPs eluted from pneumococci as an experimental vaccine. Since PspA shows variability between isolates, we constructed strains producing different PspAs. We used the nonencapsulated Rx1 strain, which produces PspA from clade 2 (PspA2), to generate apspA-knockout strain (Rx1 ΔpspA) and strains expressing PspA from clade 1 (Rx1pspA1) and clade 4 (Rx1pspA4). We grew Rx1, Rx1 ΔpspA, Rx1pspA1, and Rx1pspA4in Todd-Hewitt medium containing 0.5% yeast extract and washed cells in 2% choline chloride (CC). SDS-PAGE analysis of the proteins recovered by a CC wash showed few bands, and the CBPs PspA and PspC (pneumococcal surface protein C) were identified by mass spectrometry analysis. Subcutaneous immunization of mice with these full-length native proteins without adjuvant led to significantly higher rates of survival than immunization with diluent after an intranasal lethal challenge with two pneumococcal strains and also after a colonization challenge with one strain. Importantly, immunization with recombinant PspA4 (rPspA4) without adjuvant did not elicit significant protection.
Design and Implementation of an External Quality Assessment Program for HIV Viral Load Measurements Using Dried Blood Spots
Authors: Prach Lisa M. , Puren Adrian , Lippman Sheri A. , Carmona Sergio , Stephenson Sophie , Cutler Ewalde , Barnhart Scott , Liegler Teri 
Published: December 2014

ABSTRACT
An external quality assurance program was developed for HIV-1 RNA viral load measurements taken from dried blood spots using a reference panel and field-collected specimens. The program demonstrated that accurate and reproducible quantitation can be obtained from field-collected specimens. Residual proviral DNA may confound interpretation in virologically suppressed subjects.
Rapid Identification of Microorganisms from Sterile Body Fluids by Use of FilmArray
Authors: Altun Osman , Almuhayawi Mohammed , Ullberg Måns , Özenci Volkan 
Published: December 2014

ABSTRACT
We evaluated the clinical performance of the FilmArray blood culture identification (BCID) panel in the identification of microorganisms from positive blood culture bottles inoculated with sterile body fluids. All organisms included in the FA BCID panel were accurately identified in 84/84 (100%) and 18/24 (75%) samples with mono- and polymicrobial growth, respectively.
A Human Herpesvirus 6A-Encoded MicroRNA: Role in Viral Lytic Replication
Authors: Nukui Masatoshi , Mori Yasuko , Murphy Eain A. 
Published: December 2014

ABSTRACT
Human herpesvirus 6A (HHV-6A), a member of the betaherpesvirus family, is associated with several human diseases. Like all herpesviruses, HHV-6A establishes a lifelong, latent infection in its host. Reactivation of HHV-6A is frequent within the immunosuppressed and immunocompromised populations and results in lytic viral replication within multiple organs, often leading to severe disease. MicroRNAs (miRNAs) are key regulators of multiple cellular processes that regulate the translation of specific transcripts. miRNAs carried by herpesviruses play important roles in modulating the host cell, thereby facilitating a suitable environment for productive viral infection and/or latency. Currently, there are approximately 150 known human herpesvirus-encoded miRNAs, although an miRNA(s) encoded by HHV-6A has yet to be reported. We hypothesized that HHV-6A, like other members of the human herpesvirus family, encodes miRNAs, which function to promote viral infection. We utilized deep sequencing of small RNA species isolated from cells harboring HHV-6A to identify five novel small noncoding RNA species that originate from the viral genome, one of which has the characteristics of a viral miRNA. These RNAs are expressed during productive infection by either bacterial artificial chromosome (BAC)-derived virus in Jjhan cells or wild-type HHV-6A strain U1102 virus in HSB2 cells and are associated with the RNA induced silencing complex (RISC) machinery. Growth analyses of mutant viruses that lack each individual miRNA revealed that a viral miRNA candidate (miR-U86) targets the HHV-6A IE gene U86, thereby regulating lytic replication. The identification and biological characterization of this HHV-6A-specific miRNA is the first step to defining how the virus regulates its life cycle.

IMPORTANCE
A majority of the human population is infected with human herpesvirus 6A (HHV-6A), a betaherpesvirus family member. Infections usually occur in young children, and upon resolution, the virus remains in a latent state within the host. Importantly, during times of weakened immune responses, the virus can reactivate and is correlated with significant disease states. Viruses encode many different types of factors that both undermine the host antiviral response and regulate viral replication, including small RNA species called microRNAs (miRNAs). Here we report that HHV-6A encodes at least one miRNA, which we named miR-U86. We have characterized the requirement of this viral miRNA and its impact on the viral life cycle and found that it functions to regulate a viral protein important for efficient viral replication. Our data suggest that viral miRNAs are important for HHV-6A and that they may serve as an important therapeutic target to inhibit the virus.
Role of Cdk1 in the p53-Independent Abrogation of the Postmitotic Checkpoint by Human Papillomavirus E6
Authors: Zhang Weifang , Liu Yingwang , Zhao Ning , Chen Hanxiang , Qiao Lijun , Zhao Weiming , Chen Jason J. 
Published: December 2014

ABSTRACT

Specific types of human papillomavirus (HPV) are strongly associated with the development of cervical carcinoma. The HPV E6 oncoprotein from HPV degrades p53 and abrogates cell cycle checkpoints. Nonetheless, functional p53 has been observed in cervical cancer. We have previously identified a p53-independent function of E6 in attenuating the postmitotic G
1
-like checkpoint that can lead to polyploidy, an early event during cervical carcinogenesis that predisposes cells to aneuploidy. How E6 promotes cell cycle progression in the presence of p53 and its target, p21, remains a mystery. In this study, we examined the expression of cell cycle-related genes in cells expressing wild-type E6 and the mutant that is defective in p53 degradation but competent in abrogating the postmitotic checkpoint. Our results demonstrated an increase in the steady-state levels of G
1
- and G
2
-related cyclins/Cdks in E6-expressing keratinocytes. Interestingly, only Cdk1 remained active in E6 mutant-expressing cells while bypassing the postmitotic checkpoint. Furthermore, the downregulation of Cdk1 impaired the ability of both wild-type and mutant E6 to induce polyploidy. Our study thus demonstrated an important role for Cdk1, which binds p21 with lower affinity than Cdk2, in abrogating the postmitotic checkpoint in E6-expressing cells. We further show that E2F1 is important for E6 to upregulate Cdk1. Moreover, reduced nuclear p21 localization was observed in the E6 mutant-expressing cells. These findings shed light on the mechanisms by which HPV induces genomic instability and hold promise for the identification of drug targets.

IMPORTANCE
HPV infection is strongly associated with the development of cervical carcinoma. HPV encodes an E6 oncoprotein that degrades the tumor suppressor p53 and abrogates cell cycle checkpoints. Nonetheless, functional p53 has been observed in cervical cancer. We have recently demonstrated a p53-independent abrogation of the postmitotic checkpoint by HPV E6 that induces polyploidy. However, the mechanism is not known. In this study, we provide evidence that Cdk1 plays an important role in this process. Previously, Cdk2 was thought to be essential for the G
1
/S transition, while Cdk1 only compensated its function in the absence of Cdk2. Our studies have demonstrated a novel role of Cdk1 at the postmitotic G
1
-like checkpoint in the presence of Cdk2. These findings shed light on the mechanisms by which HPV induces genomic instability and hold promise for the identification of drug targets.
Structural Basis for the Development of Avian Virus Capsids That Display Influenza Virus Proteins and Induce Protective Immunity
Authors: Pascual Elena , Mata Carlos P. , Gómez-Blanco Josué , Moreno Noelia , Bárcena Juan , Blanco Esther , Rodríguez-Frandsen Ariel , Nieto Amelia , Carrascosa José L. , Castón José R. 
Published: December 2014

ABSTRACT

Bioengineering of viruses and virus-like particles (VLPs) is a well-established approach in the development of new and improved vaccines against viral and bacterial pathogens. We report here that the capsid of a major avian pathogen, infectious bursal disease virus (IBDV), can accommodate heterologous proteins to induce protective immunity. The structural units of the ∼70-nm-diameter T=13 IBDV capsid are trimers of VP2, which is made as a precursor (pVP2). The pVP2 C-terminal domain has an amphipathic α helix that controls VP2 polymorphism. In the absence of the VP3 scaffolding protein, 466-residue pVP2 intermediates bearing this α helix assemble into genuine VLPs only when expressed with an N-terminal His
6
tag (the HT-VP2-466 protein). HT-VP2-466 capsids are optimal for protein insertion, as they are large enough (cargo space, ∼78,000 nm
3
) and are assembled from a single protein. We explored HT-VP2-466-based chimeric capsids initially using enhanced green fluorescent protein (EGFP). The VLP assembly yield was efficient when we coexpressed EGFP-HT-VP2-466 and HT-VP2-466 from two recombinant baculoviruses. The native EGFP structure (∼240 copies/virion) was successfully inserted in a functional form, as VLPs were fluorescent, and three-dimensional cryo-electron microscopy showed that the EGFP molecules incorporated at the inner capsid surface. Immunization of mice with purified EGFP-VLPs elicited anti-EGFP antibodies. We also inserted hemagglutinin (HA) and matrix (M2) protein epitopes derived from the mouse-adapted A/PR/8/34 influenza virus and engineered several HA- and M2-derived chimeric capsids. Mice immunized with VLPs containing the HA stalk, an M2 fragment, or both antigens developed full protection against viral challenge.

IMPORTANCE
Virus-like particles (VLPs) are multimeric protein cages that mimic the infectious virus capsid and are potential candidates as nonliving vaccines that induce long-lasting protection. Chimeric VLPs can display or include foreign antigens, which could be a conserved epitope to elicit broadly neutralizing antibodies or several variable epitopes effective against a large number of viral strains. We report the biochemical, structural, and immunological characterization of chimeric VLPs derived from infectious bursal disease virus (IBDV), an important poultry pathogen. To test the potential of IBDV VLPs as a vaccine vehicle, we used the enhanced green fluorescent protein and two fragments derived from the hemagglutinin and the M2 matrix protein of the human murine-adapted influenza virus. The IBDV capsid protein fused to influenza virus peptides formed assemblies able to protect mice against viral challenge. Our studies establish the basis for a new generation of multivalent IBDV-based vaccines.
Measles Virus Mutants Possessing the Fusion Protein with Enhanced Fusion Activity Spread Effectively in Neuronal Cells, but Not in Other Cells, without Causing Strong Cytopathology
Authors: Watanabe Shumpei , Ohno Shinji , Shirogane Yuta , Suzuki Satoshi O. , Koga Ritsuko , Yanagi Yusuke 
Published: December 2014

ABSTRACT
Subacute sclerosing panencephalitis (SSPE) is caused by persistent measles virus (MV) infection in the central nervous system (CNS). Since human neurons, its main target cells, do not express known MV receptors (signaling lymphocyte activation molecule [SLAM] and nectin 4), it remains to be understood how MV infects and spreads in them. We have recently reported that fusion-enhancing substitutions in the extracellular domain of the MV fusion (F) protein (T461I and S103I/N462S/N465S), which are found in multiple SSPE virus isolates, promote MV spread in human neuroblastoma cell lines and brains of suckling hamsters. In this study, we show that hyperfusogenic viruses with these substitutions also spread efficiently in human primary neuron cultures without inducing syncytia. These substitutions were found to destabilize the prefusion conformation of the F protein trimer, thereby enhancing fusion activity. However, these hyperfusogenic viruses exhibited stronger cytopathology and produced lower titers at later time points in SLAM- or nectin 4-expressing cells compared to the wild-type MV. Although these viruses spread efficiently in the brains of SLAM knock-in mice, they did not in the spleens. Taken together, the results suggest that enhanced fusion activity is beneficial for MV to spread in neuronal cells where no cytopathology occurs, but detrimental to other types of cells due to strong cytopathology. Acquisition of enhanced fusion activity through substitutions in the extracellular domain of the F protein may be crucial for MV's extensive spread in the CNS and development of SSPE.

IMPORTANCE
Subacute sclerosing panencephalitis (SSPE) is a fatal disease caused by persistent measles virus (MV) infection in the central nervous system (CNS). Its cause is not well understood, and no effective therapy is currently available. Recently, we have reported that enhanced fusion activity of MV through the mutations in its fusion protein is a major determinant of efficient virus spread in human neuronal cells and brains of suckling hamsters. In this study, we show that those mutations render the conformation of the fusion protein less stable, thereby making it hyperfusogenic. Our results also show that enhanced fusion activity is beneficial for MV to spread in the CNS but detrimental to other types of cells in peripheral tissues, which are strongly damaged by the virus. Our findings provide important insight into the mechanism for the development of SSPE after MV infection.
Effect of Electroacupuncture on the Inflammatory Response in Patients with Acute Pancreatitis: An Exploratory Study
Authors: Zhu Shi-Feng , Guo Hui , Zhang Rong-Rong , Zhang Yumei , Li Juan , Zhao Xian-Lin , Chen Tian-Rong , Wan Mei-Hua , Chen Guang-Yuan , Tang Wen-Fu 
Published: December 2014

Objectives To examine the effects of electroacupuncture (EA) on inflammatory responses in patients with acute pancreatitis (AP). Methods Eighty patients with mild or severe AP were randomly allocated to a control group or an EA group. All patients were managed conservatively. In addition, the EA group received acupuncture for 30 min per day for 7 days at bilateral points ST36, LI4, TE6, ST37 and LR3. Interleukin (IL)-6, IL-10 and C-reactive protein (CRP) levels were measured on admission and on day 7. The time to re-feeding and length of stay in hospital were also recorded. Results A total of 58 patients provided complete data. The characteristics of the patients in the EA and control groups were similar. After 7 days the serum concentrations of IL-10 were higher in the EA group than in the control group (mild AP: 6.2±1.2 vs 5.2±0.9 pg/mL, p<0.05; severe AP: 14.9±7.8 vs 7.9±6.3 pg/mL, p<0.05). For patients with severe AP, the CRP level in the EA group was lower than in the control group (p<0.05). Conclusions EA may reduce the severity of AP by inducing anti-inflammatory effects and reducing the time to re-feeding; however, it did not reduce the length of hospital stay. Trial Registration Number ChiCTR-TRC-13003572.