bluuebunny/crossref_metadata_2025_split huggingface dataset:
WKYMVm-Induced Activation of Formyl Peptide Receptor 2 Stimulates Ischemic Neovasculogenesis by Promoting Homing of Endothelial Colony-Forming Cells
Authors: Heo Soon Chul , Kwon Yang Woo , Jang Il Ho , Jeong Geun Ok , Yoon Jung Won , Kim Chi Dae , Kwon Sang Mo , Bae Yoe-Sik , Kim Jae Ho 
Published: October 2013

AbstractEndothelial colony-forming cells (ECFCs) are recruited to the sites of ischemic injury in order to contribute to neovascularization and repair of injured tissues. However, therapeutic potential of ECFCs is limited due to low homing and engraftment efficiency of transplanted ECFCs. The G-protein-coupled formyl peptide receptor (FPR) 2 has been implicated in regulation of inflammation and angiogenesis, while the role of FPR2 in homing and engraftment of ECFCs and neovascularization in ischemic tissues has not been fully defined. This study was undertaken to investigate the effects of WKYMVm, a selective FPR2 agonist isolated by screening synthetic peptide libraries, on homing ability of ECFCs and vascular regeneration of ischemic tissues. WKYMVm stimulated chemotactic migration, angiogenesis, and proliferation ability of human ECFCs in vitro. Small interfering RNA-mediated silencing of FPR2, but not FPR3, abrogated WKYMVm-induced migration and angiogenesis of ECFCs. Intramuscular injection of WKYMVm resulted in attenuation of severe hind limb ischemia and promoted neovascularization in ischemic limb. ECFCs transplanted via tail vein into nude mice were incorporated into capillary vessels in the ischemic hind limb, resulting in augmented neovascularization and improved ischemic limb salvage. Intramuscular injection of WKYMVm promoted homing of exogenously administered ECFCs to the ischemic limb and ECFC-mediated vascular regeneration. Silencing of FPR2 expression in ECFCs resulted in abrogation of WKYMVm-induced in vivo homing of exogenously transplanted ECFCs to the ischemic limb, neovascularization, and ischemic limb salvage. These results suggest that WKYMVm promotes repair of ischemic tissues by stimulating homing of ECFCs and neovascularization via a FPR2-dependent mechanism. Stem Cells 2014;32:779–790
New Hexanuclear Niobium Cluster Compounds with Pyridine and N‐Methylpyrrolidone Ligands: [Nb6Cl12(Pyr)6][AlCl4]2 and [Nb6Cl12(NMP)6][GaCl4]2
Authors: Flemming Anke , König Jonas , Köckerling Martin 
Published: October 2013

AbstractThe study is focused on the preparation, characterization, and structure elucidation of two new molecular cluster complexes of niobium, one with an aromatic N donor ligand, the other one with an O‐carboxamide ligand: [Nb6Cl12(Pyr)6][AlCl4]2 (1) (Pyr = pyridine) and [Nb6Cl12(NMP)6][GaCl4]2 (2) (NMP = N‐methyl‐pyrrolidone). The synthesis of such substituted niobium cluster compounds, starting from easily accessible A4I[Nb6Cl18] (AI = alkali metal cation) materials is best done using a two‐step protocol. First the solid state precursor is heated together with acetonitrile and in the second step the ligand exchange is performed. The molecular structures of 1 and 2 in the solid state were determined by single‐crystal X‐ray diffraction. The infrared spectrum of 1 was recorded as well.
Patient Blood Management: Use of Topical Hemostatic and Sealant Agents
Authors: Ozawa Sherri 
Published: October 2013

AbstractPatient blood management is the scientific use of safe, effective medical and surgical techniques designed to conserve blood, prevent anemia, decrease bleeding, and optimize coagulation in an effort to improve patient outcomes. Perioperative and primary care nurses play a vital role in promoting and making the best use of patient blood management and can play a key role in implementing effective strategies that decrease or eliminate patient exposure to allogeneic blood. The fast and effective minimization of intraoperative bleeding is integral in an effective blood management program. Topical hemostatic and sealant agents can be used to improve blood conservation, reduce overall procedure time, and contribute to faster patient recovery based on specific clinical situations. The proper selection of hemostatic agents can greatly influence the patient's clinical outcomes.
Safety Planning for Intraoperative Magnetic Resonance Imaging
Authors: Hemingway Maureen , Kilfoyle Marguerite 
Published: October 2013

AbstractAn intraoperative magnetic resonance imaging (MRI) suite (ie, a type of hybrid OR) is a high‐risk zone that requires well‐defined safety procedures to avoid adverse events related to magnetic forces. At one facility, the opening of an MRI suite necessitated the creation of a safety plan to establish guidelines, procedures, education, and nursing care specific to the use of MRI technology in the operative environment. Formation of a steering committee enabled a multidisciplinary approach to planning and implementation. The addition of two new perioperative nursing roles (ie, MRI control room monitor, MRI safety nurse) addressed staffing challenges related to strictly enforcing MRI safety procedures and delineating duties different from those of the RN circulator. Benefits of a safe approach to an MRI‐integrated operative setting included the elimination of an entire surgical experience for patients who underwent additional resection of the tumor during their initial surgical procedure instead of postoperatively or during a subsequent return to the OR.
Standardization of rehabilitation after limb salvage surgery for sarcomas improves patients' outcome
Authors: Shehadeh Ahmad , Dahleh Mostafa El , Salem Ahmed , Sarhan Yousef , Sultan Iyad , Henshaw Robert M , Aboulafia Albert J 
Published: October 2013

BACKGROUND AND OBJECTIVE:
The purpose of this study is to establish a standardized postoperative rehabilitation protocol following limb salvage surgery (LSS) in patients with primary bone sarcoma in five major anatomical locations: distal femur, proximal tibia, proximal and total femur, humerus and shoulder girdle and pelvic resections.

SETTING AND DESIGN:
Retrospective study.

PATIENTS AND METHODS:
All LSSs were performed by an orthopedic oncology surgeon, and rehabilitation of all patients was based on a devised standardized rehabilitation protocol. Patient outcomes were measured using the modified Musculoskeletal Tumor Society–International Symposium on the Limb Salvage (MSTS–ISOLS) scoring system.

RESULTS:
A total of 59 patients received LSS in the above mentioned locations; endoprostheses were used in 49, bone allograft in five, while no replacements were made in five patients. At a mean follow-up of 24 months, the mean modified MSTS–ISOLS score for all patients was 87% (95% CI; 0.85–0.89). The highest scores were encountered for patients with distal femur replacement: 93% (95% CI; 0.91–0.95). Seven patients had interruption of more than six weeks in their rehabilitation and had a mean score of 71% (95% CI; 0.64–0.82).

CONCLUSION:
The proposed rehabilitation protocol is a comprehensive, organized and applicable guideline to be used after performing LSS at the above mentioned anatomical locations. The use of standardized rehabilitation protocol resulted in improved patient functional outcome.
Mesenchymal stem cells promote leukaemic cells aberrant phenotype from B-cell acute lymphoblastic leukaemia
Authors: Rodríguez-Pardo Viviana M , Aristizabal José A , Jaimes Diana , Quijano Sandra M , Reyes Iliana de los , Herrera María Victoria , Solano Julio , Vernot Jean Paul 
Published: October 2013

BACKGROUND AND OBJECTIVES:
The role of bone marrow-mesenchymal stem cells (BM-MSC) in leukae-mic cell control is controversial. The purpose of this work was to evaluate BM-MSC role regarding the viability, proliferation and immunophenotype of normal B-cell precursors from control (Ct) patients and leukaemic cells from B-acute lymphoblastic leukaemia (B-ALL) patients.

PATIENTS AND METHODS:
BM-MSC were isolated and characterised from voluntary donors. Mononuclear cells isolated from Ct and B-ALL bone marrow samples were cultured in the presence or absence of BM-MSC for 7 days. Cell viability was determined with LIVE/DEAD and proliferation index evaluated by CFSE labelling. Cell population immunophenotypes were characterised by estimating CD19, CD10, CD20 and CD45 antigens by flow cytometry.

RESULTS:
After co-culture, B-ALL cells exhibited higher viability (20–40%) as compared to just cells (3–10%). Ct and B-ALL absolute cell counts were higher in the presence of BM-MSC (Ct: 25/mm3
cf8/mm3, B-ALL: 15/ mm3
cf3/mm3). Normal B-cell subpopulations in co-culture had increased expression of CD19 and CD10 (Pre-pre B) and CD45 and CD20 antigens (Pre-B). B-ALL cells co-cultured with BM-MSC showed an increase in CD19 and CD20, although the greatest increase was observed in the CD10 antigen.

CONCLUSIONS:
Lymphoid cell maintenance, at early stages of differentiation, was significantly promoted by BM-MSC in normal and leukaemic cells. Co-cultures also modulated the expression of antigens associated with the B-ALL asynchronous phenotype as CD10 co-expressed with CD19 and CD20. To our knowledge, this is the first time that CD10, CD19 and CD20 leukaemic antigens have been reported as being regulated by BM-MSC.
Evolution of the density self-correlation in developing Richtmyer–Meshkov turbulence
Authors: Tomkins C. D. , Balakumar B. J. , Orlicz G. , Prestridge K. P. , Ristorcelli J. R. 
Published: October 2013

AbstractTurbulent mixing in a Richtmyer–Meshkov unstable light–heavy–light (air–${\mathrm{SF} }_{6} $–air) fluid layer subjected to a shock (Mach 1.20) and a reshock (Mach 1.14) is investigated using ensemble statistics obtained from simultaneous velocity–density measurements. The mixing is driven by an unstable array of initially symmetric vortices that induce rapid material mixing and create smaller-scale vortices. After reshock the flow appears to transition to a turbulent (likely three-dimensional) state, at which time our planar measurements are used to probe the developing flow field. The density self-correlation$b= - \langle \rho v\rangle $(where$\rho $and$v$are the fluctuating density and specific volume, respectively) and terms in its evolution equation are directly measured experimentally for the first time. Amongst other things, it is found that production terms in the$b$equation are balanced by the dissipation terms, suggesting a form of equilibrium in$b$. Simultaneous velocity measurements are used to probe the state of the incipient turbulence. A length-scale analysis suggests that an inertial range is beginning to form, consistent with the onset of a mixing transition. The developing turbulence is observed to reduce non-Boussinesq effects in the flow, which are found to be small over much of the layer after reshock. Second-order two-point structure functions of the density field exhibit a power-law behaviour with a steeper exponent than the standard$2/ 3$power found in canonical turbulence. The absence of a significant$2/ 3$region is observed to be consistent with the state of the flow, and the emergence of the steeper power-law region is discussed.
Electrified coating flows on vertical fibres: enhancement or suppression of interfacial dynamics
Authors: Wray A. W. , Papageorgiou D. T. , Matar O. K. 
Published: October 2013

AbstractWe investigate the evolution and stability of a wetting viscous fluid layer flowing down the surface of a cylinder, and surrounded by a conductive gas. The inner cylinder is an electrode kept at constant voltage, and a second, concentric electrode encloses the system whose potential is allowed to vary spatially. This induces electrostatic forces at the interface in competition with surface tension and viscous stresses. Asymptotic methods are used to derive a long-wave axisymmetric model governing the interfacial position and charge density. The resulting system of equations is investigated both analytically and numerically to determine its stability characteristics in the linear and nonlinear regimes.
Wall effects on self-diffusiophoretic Janus particles: a theoretical study
Authors: Crowdy Darren G. 
Published: October 2013

AbstractWe present a theoretical investigation of the self-diffusiophoresis of a class of two-faced, two-dimensional Janus particles propelled by the production of gradients in the concentration of a solute diffusing into a surrounding fluid at zero Reynolds and Péclet numbers. Those concentration gradients produce a tangential boundary slip resulting in translation and rotation of the particle, as a consequence of the fact that it is free of both force and torque. The model Janus particles studied here have piecewise constant surface mobilities and surface activities over two faces. An isolated circular Janus particle is studied first and its speed of locomotion in free space is found analytically. Confinement effects are then investigated by placing such a Janus particle near a straight no-slip wall. The governing nonlinear dynamical system is found in explicit form. It is used to study how the geometry, location and orientation of the particle relative to the wall affect its motion. It is found that if the particles do not hit the wall in finite time they are eventually repelled away from it.
Parasites as biological tags of fish stocks: a meta-analysis of their discriminatory power
Authors: POULIN ROBERT , KAMIYA TSUKUSHI 
Published: October 2013

SUMMARYThe use of parasites as biological tags to discriminate among marine fish stocks has become a widely accepted method in fisheries management. Here, we first link this approach to its unstated ecological foundation, the decay in the similarity of the species composition of assemblages as a function of increasing distance between them, a phenomenon almost universal in nature. We explain how distance decay of similarity can influence the use of parasites as biological tags. Then, we perform a meta-analysis of 61 uses of parasites as tags of marine fish populations in multivariate discriminant analyses, obtained from 29 articles. Our main finding is that across all studies, the observed overall probability of correct classification of fish based on parasite data was about 71%. This corresponds to a two-fold improvement over the rate of correct classification expected by chance alone, and the average effect size (Zr = 0·463) computed from the original values was also indicative of a medium-to-large effect. However, none of the moderator variables included in the meta-analysis had a significant effect on the proportion of correct classification; these moderators included the total number of fish sampled, the number of parasite species used in the discriminant analysis, the number of localities from which fish were sampled, the minimum and maximum distance between any pair of sampling localities, etc. Therefore, there are no clear-cut situations in which the use of parasites as tags is more useful than others. Finally, we provide recommendations for the future usage of parasites as tags for stock discrimination, to ensure that future applications of the method achieve statistical rigour and a high discriminatory power.
Antimicrobial resistance in commensalEscherichia coliin veal calves is associated with antimicrobial drug use
Authors: BOSMAN A. B. , WAGENAAR J. A. , STEGEMAN J. A. , VERNOOIJ J. C. M. , MEVIUS D. J. 
Published: October 2013

SUMMARYThe aim of this study was to determine the association between farm management factors, including antimicrobial drug usage, and resistance in commensalEscherichia coliisolates from the faeces of white veal calves. NinetyE. coliisolates from one pooled sample per farm (n = 48) were tested for their phenotypical resistance against amoxicillin, tetracycline, cefotaxime, ciprofloxacin and trimethoprim/sulfamethoxazole (TMP/SMX). Logistic regression analysis revealed the following risk factors (P < 0·05); farmer wearing the same work clothes for several days [ciprofloxacin, odds ratio (OR) 2·6; tetracycline, OR 2·4], administration of trimethoprim-sulfonamide combinations (TMP/SMX, OR 3·0; amoxicillin, OR 3·1; tetracycline, OR 2·6), ⩾0·3 animal daily dosage per production cycle (ADD/pc), quinolones (ciprofloxacin, OR 2·8), ⩾1·3 ADD/pc, penicillins (ciprofloxacin, OR 3·3; tetracycline, OR 3·4), 20–40 ADD/pc, tetracyclines (tetracycline, OR 3·2) and >40 ADD/pc, tetracyclines (tetracycline, OR 13·1; amoxicillin, OR 6·5). In this study antimicrobial resistance in commensalE. coliwas mainly associated with antimicrobial drug use.
Semi-Strong Colouring of Intersecting Hypergraphs
Authors: BLAIS ERIC , WEINSTEIN AMIT , YOSHIDA YUICHI 
Published: October 2013

For any c ≥ 2, a c-strong colouring of the hypergraph G is an assignment of colours to the vertices of G such that, for every edge e of G, the vertices of e are coloured by at least min{c,|e|} distinct colours. The hypergraph G is t-intersecting if every two edges of G have at least t vertices in common.A natural variant of a question of Erdős and Lovász is: For fixed c ≥ 2 and t ≥ 1, what is the minimum number of colours that is sufficient to c-strong colour any t-intersecting hypergraphs? The purpose of this note is to describe some open problems related to this question.
Patent ductus arteriosus: an analysis of management
Authors: Wardle Andrew J , Osman Ahmed , Tulloh Robert , Luyt Karen 
Published: October 2013

AbstractTo compare differences in the management of the neonatal patent ductus arteriosus between neonatologists and paediatric cardiologists, physicians throughout the South-West were contacted. In treatment-refractory cases, neonatologists considered ligation less frequently than paediatric cardiologists (0% versus 40%; p<0.05) and held haemodynamic effects more important for ligation decisions [median: 5 (range 2–5) versus median: 4 (range 2–5); p<0.05]. Furthermore, 81% felt the current guidelines were insufficient.
Stereological Estimation of Orientation Distribution of Generalized Cylinders from a Unique 2D Slice
Authors: Da Costa Jean-Pierre , Oprean Stefan , Baylou Pierre , Germain Christian 
Published: October 2013

AbstractThough three-dimensional (3D) imaging gives deep insight into the inner structure of complex materials, the stereological analysis of 2D snapshots of material sections is still necessary for large-scale industrial applications for reasons related to time and cost constraints. In this paper, we propose an original framework to estimate the orientation distribution of generalized cylindrical structures from a single 2D section. Contrary to existing approaches, knowledge of the cylinder cross-section shape is not necessary. The only requirement is to know the area distribution of the cross-sections. The approach relies on minimization of a least squares criterion under linear equality and inequality constraints that can be solved with standard optimization solvers. It is evaluated on synthetic data, including simulated images, and is applied to experimental microscopy images of fibrous composite structures. The results show the relevance and capabilities of the approach though some limitations have been identified regarding sensitivity to deviations from the assumed model.
Head and neck cancer patients want us to support them psychologically in the posttreatment period: Survey results
Authors: Henry Melissa , Habib Laura-Anne , Morrison Matthew , Yang Ji Wei , Li Xuejiao Joanna , Lin Shiru , Zeitouni Anthony , Payne Richard , MacDonald Christina , Mlynarek Alexander , Kost Karen , Black Martin , Hier Michael 
Published: October 2013

AbstractObjectives:No study systematically has investigated the supportive care needs of general head and neck cancer patients using validated measures. These needs include physical and daily living needs, health system and information needs, patient care and support needs, psychological needs, and sexuality needs. Identifying the unmet needs of head and neck cancer patients is a necessary first step to improving the care we provide to patients seen in our head and neck oncology clinics. It is recommended as the first step in intervention development in the Pan-Canadian Clinical Practice Guideline of the Canadian Partnership Against Cancer (see Howell, 2009). This study aimed to identify: (1) met and unmet supportive care needs of head and neck cancer patients, and (2) variability in needs according to demographics, disease variables, level of distress, and quality-of-life domains.Methods:Participants were recruited from the otolaryngology–head and neck surgery clinics of two university teaching hospitals. Self-administered questionnaires included sociodemographic and medical questions, as well as validated measures such as the Supportive Care Needs Survey–Short Form (SCNS-SF34), the Hospital Anxiety and Depression Scale (HADS), and the Functional Assessment of Cancer Therapy–General (FACT-G) and Head and Neck Module (FACT-H&N) (quality of life measures).Results:One hundred and twenty-seven patients participated in the survey. 68% of them experienced unmet needs, and 25% revealed a clinically significant distress level on the HADS. The highest unmet needs were psychological (7 of top 10 needs). A multiple linear regression indicated a higher level of overall unmet needs when patients were divorced, had a high level of anxiety (HADS subscale), were in poor physical condition, or had a diminished emotional quality of life (FACT-G subscales).Significance of results:The results of this study highlight the overwhelming presence of unmet psychological needs in head and neck cancer patients and underline the importance of implementing interventions to address these areas perceived by patients as important. In line with hospital resource allocation and cost-effectiveness, one may also contemplate screening patients for high levels of anxiety, as well as target patients who are divorced and present low levels of physical well-being, as these patients may have more overall needs to be met.
Sympatric inhibition and niche differentiation suggest alternative coevolutionary trajectories among Streptomycetes
Authors: Kinkel Linda L , Schlatter Daniel C , Xiao Kun , Baines Anita D 
Published: October 2013

Abstract
Soil bacteria produce a diverse array of antibiotics, yet our understanding of the specific roles of antibiotics in the ecological and evolutionary dynamics of microbial interactions in natural habitats remains limited. Here, we show a significant role for antibiotics in mediating antagonistic interactions and nutrient competition among locally coexisting Streptomycete populations from soil. We found that antibiotic inhibition is significantly more intense among sympatric than allopatric Streptomycete populations, indicating local selection for inhibitory phenotypes. For sympatric but not allopatric populations, antibiotic inhibition is significantly positively correlated with niche overlap, indicating that inhibition is targeted toward bacteria that pose the greatest competitive threat. Our results support the hypothesis that antibiotics serve as weapons in mediating local microbial interactions in soil and suggest that coevolutionary niche displacement may reduce the likelihood of an antibiotic arms race. Further insight into the diverse roles of antibiotics in microbial ecology and evolution has significant implications for understanding the persistence of antibiotic inhibitory and resistance phenotypes in environmental microbes, optimizing antibiotic drug discovery and developing strategies for managing microbial coevolutionary dynamics to enhance inhibitory phenotypes.