bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of causal relationship between shoulder impingement syndrome and rotator cuff injury: a bidirectional mendelian randomization
Authors: Liu Li , Yang Fan , Liao Ying , Deng Hui , Le Dongsheng , Zhang Chao , Zhao Mailin , Liao Pingsheng , Liang Yingping 
Published: August 2024

Abstract
Backgroup
The pathogenesis of shoulder impingement syndrome (SIS) is still unclear, and its questionable causal relationship with rotator cuff (RC) injury has led to confusion in treatment. The purpose of this study was to explore the bidirectional causal relationship between SIS and RC injury.

Methods
SIS and RC injury datasets downloaded from the IEU Open GWAS project and GWAS catalog databases. Inverse variance weighted (IVW), MR Egger, Weighted median, and Weighted mode were used in this Mendelian randomization (MR) analysis. Cochran’s Q test, leave-one-out, and funnel plot method were used to evaluate heterogeneity between single nucleotide polymorphisms (SNPs). MR-Egger regression was used to test the horizontal pleiotropy of this study.

Results
The IVW method (OR = 1.189, P = 0.0059) suggest the putative causal effect of RC injury on SIS. The results of MR Egger method (OR = 1.236, P = 0.2013), weighted median method (OR = 1.097, P = 0.2428) and weighted mode method (OR = 1.013, P = 0.930) showed no statistically significant (OR = 1.069071, P = 0.6173). Heterogeneity test and horizontal pleiotropy analysis suggested that there was no significant heterogeneity and horizontal pleiotropy in the results of this MR analysis. The reverse MR analysis showed heterogeneity, and the conclusion needs to be further explored.

Conclusions
The results of MR analysis support that RC injury may be causally associated with SIS.
Financial hardship after COVID-19 infection among US Veterans: a national prospective cohort study
Authors: Govier Diana J. , Bui David P. , Hauschildt Katrina E. , Eaton Tammy L. , McCready Holly , Smith Valerie A. , Osborne Thomas F. , Bowling C. Barrett , Boyko Edward J. , Ioannou George N. , Maciejewski Matthew L. , O’Hare Ann M. , Viglianti Elizabeth M. , Bohnert Amy S. B. , Hynes Denise M. , Iwashyna Theodore J. 
Published: August 2024

Abstract
Background
Research suggests an association between COVID-19 infection and certain financial hardships in the shorter term and among single-state and privately insured samples. Whether COVID-19 is associated with financial hardship in the longer-term or among socially vulnerable populations is unknown. Therefore, we examined whether COVID-19 was associated with a range of financial hardships 18 months after initial infection among a national cohort of Veterans enrolled in the Veterans Health Administration (VHA)—the largest national integrated health system in the US. We additionally explored the association between Veteran characteristics and financial hardship during the pandemic, irrespective of COVID-19.

Methods
We conducted a prospective, telephone-based survey. Out of 600 Veterans with COVID-19 from October 2020 through April 2021 who were invited to participate, 194 Veterans with COVID-19 and 194 matched comparators without a history of infection participated. Financial hardship outcomes included overall health-related financial strain, two behavioral financial hardships (e.g., taking less medication than prescribed due to cost), and seven material financial hardships (e.g., using up most or all savings). Weighted generalized estimating equations were used to estimate risk ratios (RR) and 95% confidence intervals (CI) of financial hardship by COVID-19 status, and to assess the relationship between infection and Veteran age, VHA copay status, and comorbidity score, irrespective of COVID-19 status.

Results
Among 388 respondents, 67% reported at least one type of financial hardship since March 2020, with 21% reporting behavioral hardships and 64% material hardships; 8% reported severe-to-extreme health-related financial strain. Compared with uninfected matched comparators, Veterans with a history of COVID-19 had greater risks of severe-to-extreme health-related financial strain (RR: 4.0, CI: 1.4–11.2), taking less medication due to cost (RR: 2.9, 95% CI: 1.0–8.6), and having a loved one take time off work to care for them (RR: 1.9, CI: 1.1–3.6). Irrespective of COVID-19 status, Veterans aged < 65 years had a greater risk of most financial hardships compared with Veterans aged ≥ 65 years.

Conclusions
Health-related financial hardships such as taking less medication due to cost and severe-to-extreme health-related financial strain were more common among Veterans with a history of COVID-19 than among matched comparators. Strategies are needed to address health-related financial hardship after COVID-19.

Trial registration
NCT05394025, registered 05–27-2022.
Validation and psychometric evaluation of the Short Warwick-Edinburgh Mental Well-Being Scale (SWEMWBS) among Czech adolescents using Item Response Theory
Authors: Hanzlová Radka , Kudrnáč Aleš 
Published: August 2024

Abstract
Background
The topic of adolescent mental health is currently a subject of much debate due to the increasing prevalence of mental health problems among this age group. Therefore, it is crucial to have high-quality and validated mental well-being measurement tools. While such tools do exist, they are often not tailored specifically to adolescents and are not available in Czech language. The aim of this study is to validate and test the Czech version of the Short Warwick-Edinburgh Mental Well-Being Scale (SWEMWBS) on a large sample of Czech adolescents aged 15 to 18 years.

Methods
The analysis is based on data from the first wave of the Czech Education Panel Survey (CZEPS) and was mainly conducted using Item Response Theory (IRT), which is the most appropriate method for this type of analysis. Specifically, the Graded Response Model (GRM) was applied to the data. This comprehensive validation study also included reliability and three types of validity (construct, convergent and criterion) testing.

Results
The study found that the Czech version of the SWEMWBS for adolescents aged 15 to 18 years (N = 22,498) has good quality and psychometric properties. The data was analysed using the GRM model as it met the assumptions for the use of IRT. The estimated parameter values by GRM demonstrated good discriminant and informative power for all items, except for item 7, which showed poorer results compared to the others. However, excluding it from the scale would not enhance the overall quality of the scale. The five-category response scale functions effectively. Additionally, the results demonstrated high reliability, and all types of validity tested were also confirmed.

Conclusions
The Czech version of the SWEMWBS for adolescents has been validated as a psychometrically sound, reliable and valid instrument for measuring mental well-being. It can therefore be used with confidence in future studies.
Age assessment of unaccompanied foreign minors: an analyses of knowledge and practices among Italian pediatricians
Authors: Buonsenso Danilo , Ceccarelli Manuela , Camara Bettina , Angelone Donatella , Burzio Valentina , La Placa Simona , Valentini Piero ,   on behalf of GLNBM-SIP* and GLMM-SIMM**
Published: August 2024

Abstract
Background
Increases in migration patterns in the recent years have led to a continuously growing number of unaccompanied foreign minors (UFMs) entering Italy. As part of processing and integration, age assessment is performed by pediatricians upon request of regulatory bodies. Updated guidelines for age estimation procedures were published in 2020 in order to prioritize the well-being of the minors and the accuracy of the assessment. Nonetheless, literature suggests that the recently established multidisciplinary approach has not yet been widely adopted by physicians.

Methods
A cross-sectional exploratory survey was distributed to pediatricians in Italy in order to gauge their range of experience with UFMs and age assessment protocols.

Results
In total 344 pediatricians participated in the survey, originating from varied regions in Italy. Out of pediatricians who reported conducting age assessment procedures (38.9%), only a small fraction (14.2%) confirmed being knowledgeable about the methodology. Instead, a significant portion (28.8% and 56.4%) either had partial awareness or lacked knowledge of these procedures. These responses significantly differed when comparing hospital and outpatient pediatricians or according to their geographical area of work (p <0.05).

Conclusion
Survey responses suggest that a gap in awareness and experience regarding a multidisciplinary approach to age estimations still exists, likely in part due to a lack of resources, especially at the regional level. In the future, efforts towards the education of professionals and mobilization of resources for investment in the field will be crucial for the improvement of work with UFMs and other migrant populations.
Melon: metagenomic long-read-based taxonomic identification and quantification using marker genes
Authors: Chen Xi , Yin Xiaole , Shi Xianghui , Yan Weifu , Yang Yu , Liu Lei , Zhang Tong 
Published: August 2024

AbstractLong-read sequencing holds great potential for characterizing complex microbial communities, yet taxonomic profiling tools designed specifically for long reads remain lacking. We introduce Melon, a novel marker-based taxonomic profiler that capitalizes on the unique attributes of long reads. Melon employs a two-stage classification scheme to reduce computational time and is equipped with an expectation-maximization-based post-correction module to handle ambiguous reads. Melon achieves superior performance compared to existing tools in both mock and simulated samples. Using wastewater metagenomic samples, we demonstrate the applicability of Melon by showing it provides reliable estimates of overall genome copies, and species-level taxonomic profiles.
Feline leishmaniosis in the Mediterranean Basin: a multicenter study
Authors: Carbonara Mariaelisa , Iatta Roberta , Miró Guadalupe , Montoya Ana , Benelli Giovanni , Mendoza-Roldan Jairo Alfonso , Papadopoulos Elias , Lima Clara , Bouhsira Emilie , Nachum-Biala Yaarit , Decaro Nicola , Schunack Bettina , Baneth Gad , Otranto Domenico 
Published: August 2024

Abstract
Background
Cats are now recognized as competent hosts for Leishmania infantum and a blood source for sand fly vectors. Although canine leishmaniosis (CanL) is endemic in Mediterranean Basin countries, large-scale epidemiological studies are lacking for feline leishmaniosis (FeL). This study aimed to assess the prevalence of L. infantum infections, associated risk factors, clinical signs, and clinicopathological abnormalities in domestic cat populations from six Mediterranean Basin countries.

Methods
From 2019 to 2022, blood and serum samples of cats (n = 2067) living in Italy (n = 300), Greece (n = 297), Portugal (n = 295), France (n = 231), Israel (n = 313), and Spain (n = 631) were collected along with animal data (i.e., age, sex, breed, housing conditions, and geographical origin), clinical signs, and laboratory blood test parameters. Cats were grouped according to their age as kittens (up to 1 year), young (older than 1 and younger than 7 years), mature (between 7 and 10 years), and senior (older than 10 years). Serum samples were tested for L. infantum by immunofluorescence antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA), and blood samples of seropositive cats were tested for L. infantum kinetoplast deoxyribonucleic acid (kDNA). Viral infection by feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV) was molecularly addressed in all cats enrolled. Statistical analysis was performed to evaluate the association between the risk of L. infantum infection and independent variables, and among co-infection of L. infantum with FIV and/or FeLV, clinical signs, and clinicopathological abnormalities.

Results
Overall, 17.3% (358/2067) of cats scored positive for L. infantum by serological tests. Specifically, 24.7% were from Portugal, 23.2% from Greece, 16.6% from Israel, 15% from Spain, 13.3% from France, and 12.6% from Italy. Leishmania infantum DNA was detected in 15 seropositive animals. Housing condition and FIV infection proved to be risk factors for FeL. Leishmania seropositivity was significantly associated with weight loss, lymphadenomegaly, gingivostomatitis, and oral ulcers, as well as with reduced albumin and albumin/globulin ratio, increased total globulins and total proteins, leukocytosis, and thrombocytosis.

Conclusions
This study provides, for the first time, a large-scale epidemiological survey on FeL and its clinical presentation, revealing that L. infantum circulates among domestic cats, especially shelter/free-roaming and FIV-infected animals, living in CanL endemic countries of the Mediterranean Basin.

Graphical Abstract
Evolutionary dependency of cancer mutations in gene pairs inferred by nonsynonymous-synonymous mutation ratios
Authors: Han Dong-Jin , Kim Sunmin , Lee Seo-Young , Moon Youngbeen , Kang Su Jung , Yoo Jinseon , Jeong Hye Young , Cho Hae Jin , Jeon Jeong Yang , Sim Byeong Chang , Kim Jaehoon , Lee Seungho , Xi Ruibin , Kim Tae-Min 
Published: August 2024

Abstract
Background
Determining the impact of somatic mutations requires understanding the functional relationship of genes acquiring mutations; however, it is largely unknown how mutations in functionally related genes influence each other.

Methods
We employed non-synonymous-to-synonymous or dNdS ratios to evaluate the evolutionary dependency (ED) of gene pairs, assuming a mutation in one gene of a gene pair can affect the evolutionary fitness of mutations in its partner genes as mutation context. We employed PanCancer- and tumor type-specific mutational profiles to infer the ED of gene pairs and evaluated their biological relevance with respect to gene dependency and drug sensitivity.

Results
We propose that dNdS ratios of gene pairs and their derived cdNS (context-dependent dNdS) scores as measure of ED distinguishing gene pairs either as synergistic (SYN) or antagonistic (ANT). Mutation contexts can induce substantial changes in the evolutionary fitness of mutations in the paired genes, e.g., IDH1 and IDH2 mutation contexts lead to substantial increase and decrease of dNdS ratios of ATRX indels and IDH1 missense mutations corresponding to SYN and ANT relationship with positive and negative cdNS scores, respectively. The impact of gene silencing or knock-outs on cell viability (genetic dependencies) often depends on ED, suggesting that ED can guide the selection of candidates for synthetic lethality such as TCF7L2-KRAS mutations. Using cell line-based drug sensitivity data, the effects of targeted agents on cell lines are often associated with mutations of genes exhibiting ED with the target genes, informing drug sensitizing or resistant mutations for targeted inhibitors, e.g., PRSS1 and CTCF mutations as resistant mutations to EGFR and BRAF inhibitors for lung adenocarcinomas and melanomas, respectively.

Conclusions
We propose that the ED of gene pairs evaluated by dNdS ratios can advance our understanding of the functional relationship of genes with potential biological and clinical implications.
Can heat conditions affect the heart rate responses, perception of effort, and technical performance of young male football players during small-sided games? a comparative study
Authors: Kang ZhiHui , Chen Zhongju , Liu GuiYang 
Published: August 2024

Abstract
Background
Soccer coaches often employ small-sided games (SSGs) to elicit both physiological and technical responses from players. However, numerous contextual factors can influence the outcomes of these games. This comparative study aimed to investigate how environmental temperature (< 21ºC and > 29ºC) impacts heart rate responses, perception of effort, and technical performance in young male football players during SSGs.

Methods
This study compares temperatures below 21ºC (∼ 20.4 ± 0.4ºC) with temperatures above 29ºC (∼ 29.7 ± 0.6ºC). This repeated measures study design involved 60 male football players at a trained/developmental level, selected from under-16 and under-19 teams. It aimed to assess the effects of the 3v3 format, conducted repeatedly under conditions of 21ºC and above 29ºC. Throughout the games, mean heart rate responses (HRmean), measured via heart rate sensors; rate of perceived exertion (RPE), assessed using the CR-10 Borg scale; and successful passes and lost balls, tracked through an ad hoc observational analysis tool, were monitored.

Results
No significant interactions were observed (time*age group) in meanHR (F = 0.159; p = 0.691; $$\:{\eta\:}_{p}^{2}$$=0.003), RPE (F=0.646; p=0.425; $$\:{\eta\:}_{p}^{2}$$=0.011), number of passes completed (F=0.204; p=0.654; $$\:{\eta\:}_{p}^{2}$$=0.003), and number of lost balls (F = 0.157; p = 0.694; $$\:{\eta\:}_{p}^{2}$$=0.003). Overall, significantly higher heart rate responses in mean HR (p<0.001) and RPE (p<0.001) were observed at temperatures above 29ºC, while significantly more passes were completed at temperatures below 21ºC (p<0.001).

Conclusions
Heat conditions significantly intensified the psychophysiological responses in players, concurrently leading to a significant impairment in the number of passes. Coaches should contemplate implementing mitigation strategies to avert performance declines during heat conditions when utilizing SSGs.
Association between thermogenic brown fat and genes under positive natural selection in circumpolar populations
Authors: Ishida Yuka , Matsushita Mami , Yoneshiro Takeshi , Saito Masayuki , Nakayama Kazuhiro 
Published: August 2024

Abstract
Background
Adaptation to cold was essential for human migration across Eurasia. Non-shivering thermogenesis through brown adipose tissue (BAT) participates in cold adaptation because some genes involved in the differentiation and function of BAT exhibit signatures of positive natural selection in populations at high latitudes. Whether these genes are associated with the inter-individual variability in BAT thermogenesis remains unclear. In this study, we evaluated the potential associations between BAT activity and single nucleotide polymorphisms (SNPs) in candidate gene regions in East Asian populations.

Methods
BAT activity induced by mild cold exposure was measured in 399 healthy Japanese men and women using fluorodeoxyglucose-positron emission tomography and computed tomography (FDG-PET/CT). The capacity for cold-induced thermogenesis and fat oxidation was measured in 56 men. Association analyses with physiological traits were performed for 11 SNPs at six loci (LEPR, ANGPTL8, PLA2G2A, PLIN1, TBX15-WARS2, and FADS1) reported to be under positive natural selection. Associations found in the FDG-PET/CT population were further validated in 84 healthy East Asian men and women, in whom BAT activity was measured using infrared thermography. Associations between the SNP genotypes and BAT activity or other related traits were tested using multiple logistic and linear regression models.

Results
Of the 11 putative adaptive alleles of the six genes, two intronic SNPs in LEPR (rs1022981 and rs12405556) tended to be associated with higher BAT activity. However, these did not survive multiple test comparisons. Associations with lower body fat percentage, plasma triglyceride, insulin, and HOMA-IR levels were observed in the FDG-PET/CT population (P < 0.05). Other loci, including TBX15-WARS2, which is speculated to mediate cold adaptation in Greenland Inuits, did not show significant differences in BAT thermogenesis.

Conclusions
Our results suggest a marginal but significant association between LEPR SNPs. However, robust supporting evidence was not established for the involvement of other loci under positive natural selection in cold adaptation through BAT thermogenesis in East Asian adults. Given the pleiotropic function of these genes, factors other than cold adaptation through BAT thermogenesis, such as diet adaptation, may contribute to positive natural selection at these loci.
Engineering microscopic delivery systems: a review of dissolving microneedle design, fabrication, and function
Authors: Dave Roshan , Shinde Swapnali , Kalayil Nisha , Budar Aarati 
Published: August 2024

AbstractDissolving microneedles (DMNs) represent an innovative advancement in drug delivery and skincare technologies, offering significant advantages compared to traditional needles. This paper presents an overview of the historical evolution of microneedles and the rise of dissolving types, exploring their definition, concept, and diverse clinical applications such as vaccinations, drug delivery, and skincare treatments. Design and manufacturing considerations cover the materials employed, fabrication techniques, and methods for characterizing DMNs, focusing on aspects like mechanical strength, dissolution rate, and delivery efficiency. The mechanism of action section examines skin penetration mechanics, the process of microneedle dissolution, controlled release of active compounds, and considerations of biocompatibility and safety. Recent developments in DMNs encompass technological advancements, improved delivery systems, and updates on clinical trials and studies. Challenges and opportunities in scaling up production, overcoming market adoption barriers, and future research directions are discussed, aiming to address unmet medical needs and expand applications. In summary, DMNs have the potential to transform drug delivery and skincare treatments, with ongoing advancements aimed at tackling current challenges and unlocking new opportunities for enhanced healthcare outcomes.
Graphical Abstract
Damage and restoration technology of historic buildings of brick and wood structures: a review
Authors: Hao Yunhong , Yao Zhonghe , Wu Rigen , Bao Yuanyuan 
Published: August 2024

Abstract
Historical buildings carry the key technologies and practical values throughout the development of architectural heritage. Investigating the restoration techniques for historical buildings under various forms of damage is an essential step in the conservation and utilization of these structures. Utilizing Web of Science (WOS) and China National Knowledge Infrastructure (CNKI) as the primary databases, this study employs the PRISMA methodology to search and screen relevant research literature. A comprehensive review of the retained literature is conducted, analyzing publication trends, co-citation networks of authors and keywords, among other characteristics. To enhance the visibility of restoration techniques, three-dimensional modeling diagrams are created using actual case information of material or component damage as a reference, thereby increasing the referential value of the review content on historical building restoration techniques. The study provides a thorough review of over 30 restoration techniques corresponding to 9 types of damage from the perspectives of historical building walls and wooden components. Additionally, new materials or technologies mentioned in the retrieved literature are categorized; for instance, in the context of moisture-proof treatment for brick masonry, traditional methods are explained, and new research on electrochemical desalination and lipid-based waterproof materials is discussed and evaluated. Finally, given the multifaceted value and the variable mechanisms of damage in historical buildings, an analysis of the restoration process and techniques reveals that the restoration of historical buildings is a complex interdisciplinary process. The development and implementation of restoration plans should adhere to the principles of cultural relic protection to ensure their reasonableness and effectiveness, while also considering sustainability and environmental adaptability to protect and reuse historical buildings, thus leveraging their cultural and historical values. Moreover, the advancement of restoration techniques for historical buildings in the future should be based on the inheritance of traditional craftsmanship and materials, while also integrating new technologies, materials, and concepts to enhance the applicability and efficiency of traditional techniques.