bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic antimicrobial activities of aqueous extract derived from olive byproduct and their modes of action
Authors: Kim Yoonbin , Zhao Hefei , Avena-Bustillos Roberto J. , Wang Selina C. , Nitin Nitin 
Published: August 2024

Abstract
Background
Plant-derived antimicrobials (PDAs) are considered a viable alternative to synthetic antimicrobial agents. Diverse antimicrobial mechanisms of PDAs significantly reduce the risk of developing antimicrobial resistance. Utilization of PDAs also offers economic and environmental advantages, as they can be derived from agricultural byproducts, such as olive pomace.

Results
In this study, a green, water-based, ultrasound-assisted extraction (UAE) was deployed to obtain aqueous olive pomace extract (OPE) from dry olive pomace. Total phenolic content, extraction yield, chemical compositions, and antimicrobial activities of OPE were evaluated. In addition, the potential synergistic interaction between the phenolic components in OPE and the antimicrobial mechanisms underlying the synergistic interaction were characterized. The results show that ca. 25 mg GAE/g of extraction yields were achieved by the UAE of dry olive pomace. Based on the high-performance liquid chromatography (HPLC) analysis, diverse phenolic compounds such as gallic acid (GA), hydroxytyrosol (HT), and 4-hydroxyphenylacetic acid (4-HPA) were identified in OPE. OPE exhibited strong antimicrobial activities, and 0.2 mg GAE/mL of OPE achieved > 5 log reductions of Escherichia coli O157:H7 and Listeria innocua cells within 30 min of treatment. A 3D isobologram analysis demonstrated that OPE exhibited strong synergistic antimicrobial activities, compared to those of individual phenolic components (GA, HT, or 4-HPA), showing interaction index (γ) of 0.092 and 0.014 against E. coli O157:H7 and L. innocua, respectively (γ < 1: synergistic activity). Antimicrobial mechanism analyses revealed that phenolic components in OPE exerted strong synergistic activities through diverse modes of action, and increased levels of oxidative stress, membrane damage, and decreased levels of metabolic activities were observed in the OPE-treated bacterial cells.

Conclusions
These findings demonstrate an approach for valorizing agricultural byproducts to develop plant byproduct-based antimicrobials with strong synergistic activities. Multiple modes of action of this byproduct extract may enable the control of diverse microbes in food and agriculture systems.

Graphical abstract
Eco-friendly synthesis and antibacterial potential of chitosan crosslinked-EDTA silver nanocomposite (CCESN)
Authors: Atanda S. A. , Shaibu R. O. , Agunbiade F. O. 
Published: August 2024

AbstractThis study presents a novel and eco-friendly approach for synthesizing silver nanocomposite at room temperature. The method utilizes chitosan derived from snail (Archachatina marginata) shell waste crosslinked with EDTA as a combined reducing and capping agent. The existence of silver nanoparticles in the composite was confirmed by transmission electron microscopy (TEM), scanning electron microscopy (SEM), X-ray diffractometry (XRD), energy dispersive X-ray (EDX), energy dispersive X-ray fluorescence (EDXRF) and thermogravimetric analysis (TGA). The TEM, SEM, XRD, and analyses revealed that the silver nanoparticle has a face-centered cubic structure with an average size of 45.30 nm respectively. EDX and EDXRF showed characteristic silver peaks confirming the formation of silver nanoparticles in the composite while TGA indicated that silver nanoparticles contributed to good thermal stability of the composite. The formation of silver nanoparticles was indicated by a brown color transformation and an ultraviolet visible (UV Vis) absorption peak at 435 nm. The synthesized nanocomposite demonstrated promising antibacterial activity against both Staphylococcus saprophyticus DSM 18669 and Escherichia coli O157 strains, with S. saprophyticus showing higher susceptibility. This highlights the potential of chitosan-EDTA silver nanocomposites as alternative antimicrobial agents.
influence of lifestyle on female fertility. Review of the literature
Authors: Śmigielska-Mikołajczyk Maria Janina , Szostak Agata , Szeliga Anna , Szopińska Kinga , Graca Magdalena , Korta Karolina , Oluszczak Karolina , Dyląg Liliana , Wawszkowicz Konrad , Łowicka Weronika 
Published: August 2024

Introduction
Women who want to get pregnant soon often ask their doctors how to prepare best. During such a visit, doctors usually mention folic acid supplementation, a check-up with the dentist and gynaecologist or vaccinations. However, it is also worth pointing out the importance of lifestyle factors that affect fertility and fetal development. Modifying daily habits can improve fertility naturally and make getting pregnant faster. An increasing number of studies are being published on the importance of nutrition, physical activity, sleep, stress, stimulants and other factors on women's reproductive health. This article is a summary of the current state of knowledge in this area.

Aim of the study
The aim of this study is to review the literature and summarize current state of knowledge on the association between lifestyle factors and female reproductive function.

Material and methods of research
This literature review based on researches from PubMed platform. We searched articles describing connection between potentially modifiable lifestyle factors and women’s fertility. Search terms included keywords: „lifestyle”, „female fertility”, „obesity”, „sleep”, „nutrition”, „diet”, „stress”, „physical activity”, „smoking”, „alcohol”, „weight loss”, „reproductive health”.
The Core of Housing Markets from an Agent’s Perspective: Is It Worth Sprucing up Your Home?
Authors: Schlotter Ildikó , Biró Péter , Fleiner Tamás 
Published: August 2024

We study housing markets as introduced by Shapley and Scarf. We investigate the computational complexity of various questions regarding the situation of an agent a in a housing market H: we show that it is [Formula: see text]-hard to find an allocation in the core of H in which (i) a receives a certain house, (ii) a does not receive a certain house, or (iii) a receives a house other than a’s own. We prove that the core of housing markets respects improvement in the following sense: given an allocation in the core of H in which agent a receives a house h, if the value of the house owned by a increases, then the resulting housing market admits an allocation in its core in which a receives either h or a house that a prefers to h; moreover, such an allocation can be found efficiently. We further show an analogous result in the Stable Roommates setting by proving that stable matchings in a one-sided market also respect improvement. Funding: This work was supported by the Hungarian Scientific Research Fund [Grants K124171, K128611]. I. Schlotter is supported by the Hungarian Academy of Sciences under its Momentum Programme (LP2021-2) and its János Bolyai Research Scholarship. The research reported in this paper and carried out by T. Fleiner at the Budapest University of Technology and Economics was supported by the “TKP2020, National Challenges Program” of the National Research Development and Innovation Office [BME NC TKP2020 and OTKA K143858] and by the Higher Education Excellence Program of the Ministry of Human Capacities in the frame of the Artificial Intelligence research area of the Budapest University of Technology and Economics (BME FIKP-MI/SC). P. Biró gratefully acknowledges financial support from the Hungarian Scientific Research Fund, OTKA [Grant K143858] and the Hungarian Academy of Sciences [Momentum Grant LP2021-2].
Pengaruh Model Pembelajaran Problem-Based Learning Berbantuan Simulasi PhET terhadap Hasil Belajar Siswa
Authors: Suryani Siti , Rusdin Muhamad Epi , Koten Florentinus P.N. 
Published: August 2024

Penelitian ini mengkaji efektivitas model pembelajaran Problem based Learning (PBL) yang didukung oleh media simulasi Physics Education Technology (PhET ) terhadap hasil belajar siswa dalam mata pelajaran fisika. Latar belakang penelitian ini didasarkan pada masalah pembelajaran fisika yang masih berfokus pada guru serta kurangnya penggunaan teknologi di SMP Muhammadiyah Waipare. PBL dan PhET diharapkan dapat meningkatkan keterlibatan, pemahaman konsep, dan hasil belajar siswa. Penelitian ini menggunakan metode kuantitatif dengan desain quasi-experimental. Sampel penelitian adalah 40 siswa dari SMP Muhammadiyah Waipare, dengan 20 siswa di kelas eksperimen yang menggunakan PBL berbantuan PhET dan 20 siswa di kelas kontrol yang menggunakan metode konvensional. Data dikumpulkan melalui observasi, tes hasil belajar, dan dokumentasi. Analisis data meliputi analisis deskriptif, uji normalitas, uji homogenitas, dan uji-T. Hasil penelitian menunjukkan bahwa nilai rata-rata posttest kelas eksperimen (82,25) lebih tinggi dibandingkan kelas kontrol (74,75). Uji normalitas (uji Kolmogorov-Smirnov) menunjukkan bahwa data posttest kelas eksperimen (nilai sig 0,012) dan kelas kontrol (nilai sig 0,008) berdistribusi normal. Uji homogenitas (Uji Levene) menunjukkan nilai sig 0.632, menandakan bahwa nilai posttest tersebut seragam. Uji-T menunjukkan nilai signifikansi 0,000, menandakan adanya pengaruh signifikan model PBL berbantuan PhET terhadap hasil belajar siswa. Kesimpulan dari penelitian ini adalah bahwa penerapan PBL dengan PhET efektif dalam meningkatkan hasil belajar fisika siswa di SMP Muhammadiyah Waipare dibandingkan dengan metode pembelajaran konvensional. Penelitian ini memberikan kontribusi penting dalam mengintegrasikan teknologi dalam pendidikan fisika dan menunjukkan potensi PBL dan PhET dalam meningkatkan hasil belajar.
Analisis Dampak Perilaku Perundungan Lisan Terhadap Peserta Didik ditinjau dari Sila Ke-2 Pancasila di SMP Negeri Moro
Authors: Marici Angela , Natsir Rodja Abdul , Wahab Mohamad Ishan 
Published: August 2024

Perilaku perundungan lisan (Bullying Verbal) bukanlah lagi menjadi fenomena baru dalam dunia pendidikan Indonesia, perilaku tersebut sering kali dialami oleh peserta didik baik itu sebagai pelaku maupun korban. Banyaknya kasus perundungan lisan tidak dapat terkuak atau diketahui karena pada umumnya perilaku ini dinilai menjadi sesuatu yang wajar dilakukan oleh sesama peserta didik. Dalam hal ini khususnya terjadi juga pada peserta didik SMP Negeri Moro perilaku perundungan lisan yang meliputi menghina bentuk fisik, perbedaan usia, jenis rambut, nama dan masih banyak hal lainnya. Oleh karena itu tujuan dari penelitian ini adalah untuk menganalisis dampak perilaku perundungan lisan terhadap peserta didik ditinjau dari sila-2 Pancasila di SMP Negeri Moro. Penelitian ini menggunakan metode penelitian kualitatif dengan pendekatan deskriptif dan teknik pengumpulan data dalam penelitian ini yaitu melalui observasi, wawancara dan dokumentasi. Teknik analisis data yang dilakukan oleh peneliti dalam melakukan penelitian yaitu reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian diketahui dampak perilaku perundungan lisan yaitu korban tidak bebas dan merasa tidak aman ketika berada di lingkungan sekolah, kebebasan dalam menyampaikan perasaannya pun direnggut, hal-hal seperti bebas untuk mengenali dirinya tidak dapat dirasakan oleh korban, karena mindset negatif yang diberikan pelaku terkait kepribadiannya menjadi sesuatu yang benar baginya, korban perilaku perundungan lisan juga tidak dapat mengasa potensi yang dimilikinya di lingkungan sekolah tersebut. Adapun upaya yang dilakukan untuk mengatasi perilaku perundungan lisan yaitu menanamkan rasa kemanusiaan dan persaudaraan dengan contoh nyata dari pancasila yaitu gotong royong, menamakan kembali rasa percaya diri korban perilaku perundungan lisan dengan terus mendampingi korban dan memberikan perilaku positif yang membangun agar korban tidak merasa sendirian.
High-Q optical metasurfaces: from quasi-BIC modulation to ultra-narrowband absorption in the near-infrared spectrum
Authors: Chai Yumei , Qi Yunping , Zhou Zihao , Shi Qiang , Luo Bing , Wang Xiangxian 
Published: August 2024

In this paper, an all-dielectric metasurface composed of silicon and silica is proposed. By breaking the symmetry of each cell structure, two quasi-bound states in the continuum (BICs) with high quality (Q) factor can be excited. The maximum Q value for quasi-BICs can reach up to 15,815, and their modulation depths and spectral contrasts can be as high as 100%. We investigate the performance using the finite element method, and found that the Fano formula based on temporal coupled mode theory fits well. Multipole decomposition results indicate that transmission peak FR1 and transmission peak FR2 are mainly controlled by electric quadrupole (EQ), while transmission peak FR3 is mainly dominated by magnetic dipole (MD). By changing the polarization angle α, switching between BIC and quasi-BIC can be achieved, and thus this metasurface exhibits effective bidirectional optical switching performance. In addition, dynamic modulators are a hot topic of research. A dynamic modulator was designed by adding two rows of single-layer graphene on both sides above the silicon layer. As a matter of application, an ultra-narrowband absorber with a dielectric–dielectric–metal (DDM) structure was constructed by introducing a sufficiently thick aluminum metal layer below the original structure. The absorptivity of absorption peak A1, absorption peak A2, and absorption peak A3 is observed to be 70%, 99%, and 82%, respectively, with Q-factors of 977, 2799, and 1351. At the same time the structure can be used for refractive index sensors. The maximum sensitivity is measured at 286 nm/RIU, and the maximum figure of merit (FOM) reaches 534RIU−1. In conclusion, the metasurface provides exploration for multifunctional devices such as optical switches, modulators, ultra-narrowband absorbers, and refractive index sensors.
Efficacy of acupuncture in the treatment of post-stroke depression: A study protocol of a randomized controlled trial
Authors: Li Menghan , Ran Dawei , Yang Xinming , Wang Yu , Zhu Qian , Song Xiaoli , Shi Lei , Du Yuzheng 
Published: August 2024

Introduction
Post-stroke depression (PSD) is an emotional disease characterized by loss of interest and depression after a stroke. Acupuncture, one of the most critical non-drug therapies for the treatment of PSD, has significant clinical efficacy, but the mechanism is not fully understood. Previous study has shown that acupuncture can reduce the level of proinflammatory cytokines and increase the level of anti-inflammatory cytokines, suggesting that regulating the dynamic balance of inflammatory cytokines may be the basis for acupuncture to improve PSD symptoms.

Methods and analysis
A total of 84 patients with PSD will be recruited and randomly assigned to one of two groups at a 1:1 ratio. Based on the conventional stroke treatment, the control group will receive XingnaoKaiqiao (XNKQ) acupuncture, and the experimental group will receive antidepressant acupuncture at the same time as XNKQ acupuncture. The intervention will last four weeks, and data will be collected before and after treatment. The Hamilton depression scale (HAMD-17) is the primary outcome measure, and the secondary outcome measures include the Self-rating Depression Scale (SDS), the National Institute of Health stroke scale (NIHSS), and the Modified Barthel Index (MBI). Serum IL-1β, IL-4, 5-HT, and BDNF will be used as laboratory indicators. The scales will be assessed at baseline, two weeks, and four weeks, and serum items will be measured at baseline and four weeks after treatment. This study will observe the clinical effect of acupuncture on PSD and the changes in serum-related inflammatory cytokines and explore the possible mechanism of acupuncture against depression from the perspective of inflammatory response.

Ethics and dissemination
This study protocol was approved by the Medical Ethics Committee of the First Teaching Hospital of Tianjin University of Traditional Chinese Medicine (item number: TYLL2023[Z]004). Findings of the trial will be disseminated through peer-reviewed journals and scientific conferences.
Conserved amino acid residues and gene expression patterns associated with the substrate preferences of the competing enzymes FLS and DFR
Authors: Choudhary Nancy , Pucker Boas 
Published: August 2024

Background
Flavonoids, an important class of specialized metabolites, are synthesized from phenylalanine and present in almost all plant species. Different branches of flavonoid biosynthesis lead to products like flavones, flavonols, anthocyanins, and proanthocyanidins. Dihydroflavonols form the branching point towards the production of non-colored flavonols via flavonol synthase (FLS) and colored anthocyanins via dihydroflavonol 4-reductase (DFR). Despite the wealth of publicly accessible data, there remains a gap in understanding the mechanisms that mitigate competition between FLS and DFR for the shared substrate, dihydroflavonols.

Results
An angiosperm-wide comparison of FLS and DFR sequences revealed the amino acids at positions associated with the substrate specificity in both enzymes. A global analysis of the phylogenetic distribution of these amino acid residues revealed that monocots generally possess FLS with Y132 (FLSY) and DFR with N133 (DFRN). In contrast, dicots generally possess FLSH and DFRN, DFRD, and DFRA. DFRA, which restricts substrate preference to dihydrokaempferol, previously believed to be unique to strawberry species, is found to be more widespread in angiosperms and has evolved independently multiple times. Generally, angiosperm FLS appears to prefer dihydrokaempferol, whereas DFR appears to favor dihydroquercetin or dihydromyricetin. Moreover, in the FLS-DFR competition, the dominance of one over the other is observed, with typically only one gene being expressed at any given time.

Conclusion
This study illustrates how almost mutually exclusive gene expression and substrate-preference determining residues could mitigate competition between FLS and DFR, delineates the evolution of these enzymes, and provides insights into mechanisms directing the metabolic flux of the flavonoid biosynthesis, with potential implications for ornamental plants and molecular breeding strategies.
Gender-specific play behavior in relation to autistic traits and behavioral difficulties at the age of seven in the SELMA study
Authors: Özel Fatih , Stratmann Marlene , Papadopoulos Fotios C. , Rüegg Joëlle , Bornehag Carl-Gustaf 
Published: August 2024

Background
Childhood gender nonconformity is related to psychological distress and behavioral difficulties. Similarly, there is evidence for a link between gender nonconformity, or gender dysphoria in some studies, and autism spectrum disorder and related traits. Our knowledge on those associations mostly originates from clinical populations, which might lead to overestimation. Thus, this study aimed to assess associations between gender nonconformity and behavioral difficulties in a population-based study.

Methods
In the Swedish Environmental Longitudinal, Mother and Child, Asthma and Allergy (SELMA) study, cross-sectional associations between gender-specific play behavior and behavioral outcomes and autistic traits were investigated among 718 children at 7-years of age. Play behavior was measured using the Preschool Activities Inventory; behavioral outcomes and autistic traits were measured with the Strengths and Difficulties Questionnaire and the Social Responsiveness Scale, respectively. Linear and logistic regression analyses were performed.

Results
Higher composite play behavior scores (indicating either increased masculine or decreased feminine play behavior) were associated with increased autistic trait scores in girls (β = 0.13; 95% confidence interval [CI] = 0.00, 0.26). Furthermore, higher composite scores were shown to be associated with behavioral difficulties in both girls (β = 0.11; 95% CI = 0.04, 0.18) and boys (β = 0.10; 95% CI = 0.02, 0.19). Additionally, higher feminine scores were related with increased problems in peer relationships in boys (β = 0.04; 95% CI = 0.00, 0.07).

Conclusions
This study suggests a link between gender nonconforming play behavior and autistic traits as well as behavioral difficulties among children in a non-clinical population, which calls attention to the necessity of supporting children with gender nonconformity from early ages.
Internet-Delivered Exposure-Based Cognitive Behavior Therapy for Irritable Bowel Syndrome: A Clinical Effectiveness Study
Authors: Wallén Hugo , Ljótsson Brjánn , Lindfors Perjohan , Forsell Erik , Hesser Hugo , Svanborg Cecilia 
Published: August 2024

INTRODUCTION:
Irritable bowel syndrome (IBS) is a common and debilitating disorder. When dietary and pharmacological interventions are not satisfactory, psychological treatment may produce good results. However, the access to such treatment is scarce, and therefore, it is of importance to make use of technical solutions. In this study, we wanted to investigate the real-world effectiveness of an Internet-delivered exposure-based cognitive behavior therapy (ECBT) for IBS and to replicate an earlier finding regarding the working mechanism of the treatment.

METHODS:
A total of 309 consecutively recruited patients from the Internet Psychiatry Clinic in Stockholm received ECBT for 12 weeks. The patients' IBS symptoms, quality of life, avoidance behaviors, and gastrointestinal symptom-specific anxiety were monitored, and we used a bivariate cross-lagged panel model to investigate time-related change in symptoms and avoidance behaviors.

RESULTS:
IBS symptoms, measured with the Gastrointestinal Symptom Rating Scale for IBS, were reduced from 48.06 (SD = 11.26) before treatment to 33.06 (SD = 10.81) 6 months after treatment (P < 0.001). The effect size (calculated by Cohen d) was 1.30 (1.08–1.51). There was a significant (P < 0.001) cross-lagged effect from reduction in avoidance behavior to reduction in symptoms but not in the reverse direction, indicating that the treatment effect is mediated by behavioral change.

DISCUSSION:
We conclude that ECBT is effective under real-world conditions, also when delivered through the Internet, and that an important treatment mechanism is the reduction of avoidance behaviors.
PROMOTING CHILD-FRIENDLY EDUCATION IN THE DIGITAL AGE: A COMMUNITY SERVICE PERSPECTIVE
Authors: Sudirman Anselmus , Eri Setiawan  , Evi Septiani  
Published: August 2024

Child-friendly education is an important program that provides children from various socio-cultural and ethnic backgrounds with safe, comfortable, and nonviolent learning activities at the family, school, and community. This community service-based article aims to describe (1) the fundamental reasons for revitalizing child-friendly education and (2) the critical principles and elements that allow children to develop their potential in the digital age fully. This program used a dialogic approach to deliver the topic under discussion, involving twenty participants, including parents and teenagers, in Mlese, Klaten Regency, Central Java Province, Indonesia. The dialogic interaction takes the form of (1) presenting material related to child-friendly education in the digital age, (2) investigating problems in small groups based solely on subtopics, and (3) openly discussing solutions to such problems. The results of data analysis from this interactive dialogue affirmed that human-to-human oral interactions enable problem-solving. Children can develop essential values of honesty, dignity, respect, and safety when key ideas and components of child-friendly education are incorporated into their school, schools and community-based platforms.