bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of mycoses on sowing qualities of Sage seeds (Salvia officinalis L.) in the Non-Chernozem soil zone of the Russian Federation
Authors: Toporishcheva M. V. , Korotkikh I. N. , Andreevskaya V. M. , Lisovoy A. M. , Eremina U. V. , Bondareva E. V. 
Published: July 2024

Relevance.Medicinal sage is a pharmacopoeial species, a valuable essential oil plant. The method of plantation establishment is predominantly seed-based, and the seed material can retain pathogenic microbiota, so the purpose of the study was to identify mycosis pathogens in the seeds of medicinal sage.Materials and methods. The study was carried out on samples from the biological collection of Salvia officinalis seeds (as part of the Unique Scientific Installation “Biological Collections of the All-Russian Research Institute of Medicinal and Aromatic Plants”). Seeds of the 1st–5th and 9th years of storage were collected from the nurseries of the Experimental Field of the All-Russian Research Institute of Medicinal and Aromatic Plants. Research to identify mycoses of sage seeds was carried out at the All-Russian Research Institute of Phytopathology in the innovative laboratory of microbiology. To study the phytopathogens of Salvia officinalis seeds, the method of artificial nutrient media was used.Results. From the study conducted, the effect of predominant pathogen was established. Fungi of the genus Alternaria, when seeds are stored in conditions with a temperature regime of +20...+22℃, have a negative effect on the sowing quality of seeds. Laboratory germination and germination energy of seeds stored in the cold room (0...+5℃) were affected by yeast, a significant decrease in sowing qualities was observed. When seeds were stored in -18℃, the inhibitory effect of temperature on pathogens was found. The dependence of total pathogen infestation on the conditions and term of seed storage was established. The highest percentage of infestation in seeds stored at room temperature was observed in the first 2 years from the time of collection. The development of pathogenic fungi of Alternaria, Phoma and Rhizopus genus was significantly reduced in seeds stored for 5 to 9 years with temperature conditions of 0...+5℃ and -18℃. Rhizopus, Cladosporium cladosporioides and Clonostachys rosea were found in the sample stored for 38 years in the freezer. The dependence of the set of pathogenic microbiota of seeds on the predecessor in the crop rotation was established. The plots from which seeds were collected, except for the sample from the Crimean zonal experimental station of VILR, have different predecessors. The seeds from the first plot showed the highest percentage of fungal infestation with similar pathogen composition. Total infestation and pathogen composition decreased in the second plot. The sample from the third plot was stored for 5 years in the freezer and no seed mycoses were detected in it.
Effect of additional inoculation with Rhizobium leguminosarum on vegetable pea plants
Authors: Putina O. V. , Putin O. V. , Zhukov V. A. , Besedin A. G. 
Published: July 2024

Relevance. Grain of vegetable pea varieties is processed at canning factories, frozen, dried, and consumed fresh. Industrial cultivation of peas can be made more environmentally friendly by using biological preparations that contain growth-stimulating bacteria, for example Rhizobium species. With additional inoculation with rhizobacteria, legumes have higher values of traits characterizing vegetative development, biochemical parameters and yield; their immunity and resistance to abiotic stress increases as well.Methodology. The aim of our study was to evaluate the effect of additional inoculation of Rhizobium leguminosarum strain 245a on biometric and physiological parameters and yield of vegetable pea varieties. The experiment was carried out at Krymsk Experiment Breeding Station – a branch of VIR (Krasnodar Region, Krymsk). We studied six commercial cultivar: Prima, Mayak, Alpha 2, Vesta, Parus (leafless morphotype) and Patriot. The seeds were treated the day before sowing with an aqueous solution of the biopreparation Rhizotorfin (produced by ECOS BIOPREPARATIONS, St.Petersburg) and with water in the control variant.Results. The use of the preparation had a growth-stimulating effect on vegetable pea plants. An increase in the length of plants, the height of attachment of the lower pod and the number of completed pods on the plant was found. An increase in the content of dry matter in the total biomass of plants, stems with leaves and pods was noted when using Rhizotorfin. Also, inoculation with rhizobacteria contributed to an increase in yield. The effectiveness of the biopreparation depended on the conditions of the year. The maximum difference between the values of the studied traits for the control and treatment variants was in 2023 - with a uniform distribution of precipitation and optimal temperatures during the growing season. A genotype-dependent reaction of vegetable pea plants to seed treatment with bacteria Rhizobium leguminosarum strain 245a was revealed. The most responsive to additional inoculation is the variety Parus of the leafless morphotype. The values of most biometric and physiological traits and yield in this genotype were significantly higher in the variant with the use of the Rhizotorfin biopreparation. Based on the results obtained, we recommend using the Rhizotorfin biopreparation when growing the variety Parus for commercial production.
Effect of inoculation with microbiological preparations on morphological characteristics and yield of vegetable soybean
Authors: Mullo Panoluisa F. E. , Romanova E. V. , Salazar Flores C. A. 
Published: July 2024

Relevance. Vegetable soybean is a special variety with high added value, consumed fresh and harvested at the stage technical ripeness (R6). Accession-A is one of the promising vegetable soybean materials, studied and identified as a result of work that was carried out jointly with the Federal Scientific Vegetable Center in the Moscow Region (FSVC) and the Agrobiotechnology Department of the Agricultural Technological Institute (ATI) of the RUDN University named after Patrice Lumumba. The use of microorganisms to promote atmospheric nitrogen fixation is a common practice in soybean production.Methodology. The purpose of this study was to evaluate the effect of two microbiological preparations based on Sinorhizobium fredii and Bradyrhizobium jaроniсum in order to identify which microbiological preparation gives the best results in terms of morphological indicators and vegetable soybean yield under the conditions region of the Non-Chernozem zone of the Russian Federation. The object of the study was accession-A of vegetable soybean. Two types of treatment were carried out: B1 – treatment with a preparation with Bradyrhizobium jaronicum (1 l/t of seeds), B2 – treatment with a preparation with Sinorhizobium fredii (1 l/t of seeds). Control without treatment (K).Results. According to statistical analysis of biometric indicators, it was found that treatment with S. fredii (B2) had the highest average values for indicators of stem diameter (11.50 mm), lower pod attachment height (11.19 cm), number of branches (3.40 pcs.) and the number of productive nodes (27.48 pcs.). Preparation B2 improved the structure of the crop, influencing the obtainment of the highest indicators of plant weight (30.47 g), seed weight per plant (15.33 g), weight of 1000 seeds (171.25 g), number of seeds per plant (98.50 pcs.), number of seeds in a bean (2.89 pcs.) and seed yield (3.11 t/ha). The use of microbiological preparations based on B. japonicum and S. fredii gave better results compared to the control treatment.
GZMK+CD8+ T cells Target A Specific Acinar Cell Type in Sjögren’s Disease
Authors: Pranzatelli Thomas JF , Perez Paola , Ku Anson , Matuck Bruno Fernandes , Huynh Khoa , Sakai Shunsuke , Abed Mehdi , Jang Shyh-Ing , Yamada Eiko , Dominick Kalie , Ahmed Zara , Oliver Amanda , Wasikowski Rachael , Easter Quinn T , Baer Alan N , Pelayo Eileen , Khavandgar Zohreh , Gupta Sarthak , Kleiner David E , Magone M Teresa , Lessard Christopher , Farris A Darise , Burbelo Peter D , Martin Daniel , Morell Robert , Zheng Changyu , Rachmaninoff Nicholas , Maldonado-Ortiz Jose , Qu Xufeng , Aure Marit H , Dezfulian Mohammad H , Lake Ross , Teichmann Sarah , Barber Daniel L , Tsoi Lam C , Sowalsky Adam G , Tyc Katarzyna M , Liu Jinze , Gudjonsson Johann E , Byrd Kevin M , Johnson Philip LF , Chiorini John A , Warner Blake M 
Published: July 2024

Abstract

Sjögren's Disease (SjD) is a systemic autoimmune disease without a clear etiology or effective therapy. Utilizing unbiased single-cell and spatial transcriptomics to analyze human minor salivary glands in health and disease we developed a comprehensive understanding of the cellular landscape of healthy salivary glands and how that landscape changes in SjD patients. We identified novel seromucous acinar cell types and identified a population of PRR4+CST3+WFDC2-seromucous acinar cells that are particularly targeted in SjD. Notably, GZMK+CD8 T cells, enriched in SjD, exhibited a cytotoxic phenotype and were physically associated with immune-engaged epithelial cells in disease. These findings shed light on the immune response's impact on transitioning acinar cells with high levels of secretion and explain the loss of this specific cell population in SjD. This study explores the complex interplay of varied cell types in the salivary glands and their role in the pathology of Sjögren's Disease.
Sero-epidemiology of Highly Pathogenic Avian Influenza viruses among wild birds in subarctic intercontinental transition zones
Authors: Gass Jonathon D. , Dusek Robert J. , Hill Nichola J. , Borkenhagen Laura K. , Hall Jeffrey S. , Hallgrimsson Gunnar Thor , Bishop Mary Anne , Ramey Andrew M. , Spivey Timothy J. , Vignisson Solvi Runar , Ragnarsdottir Sunna Bjork , Halldórsson Halldór Pálmar , Jónsson Jón Einar , Simulynas Alexa D. , Nutter Felicia B. , Puryear Wendy , Runstadler Jonathan A. 
Published: July 2024

Abstract

Background: The geographic expansion and evolution of A/Goose/Guangdong/1/1996(H5N1) (Gs/GD) lineage H5Nx highly pathogenic avian influenza (HPAI) viruses since 1996 have raised awareness of enzootic circulation among migratory birds and the potential for intercontinental transport and spread. Recent Pacific- and Atlantic-route introductions of HPAI to North America were facilitated by avian migration through subarctic zones, specifically Alaska and Iceland. This study aimed to identify recent historical patterns of exposure to HPAI viruses among birds within and migrating through both regions and evaluate how geographic, demographic, and taxonomic differences contribute to exposure risk at two intercontinental staging locations.
Methods: During 2010-2019, blood samples were obtained from captured wild migratory seabirds and waterfowl in Alaska and Iceland. All live birds were released following completion of sampling. Sampling date, species, sampling location, and age class was documented for each bird, and sex was documented when possible. Lentiviral pseudoviruses that express the influenza hemagglutinin surface glycoprotein for H5Nx HPAI and H5 low-pathogenicity avian influenza (LPAI) were constructed for use in serological assays to screen for and quantify titers of antibodies against the latter viruses. Data were analyzed to compare (a) categorical baseline ecological traits between Iceland and Alaska, and (b) ecological traits between birds identified to be seropositive and suggestive/seronegative/fully cross-reactive birds to H5Nx HPAI in Iceland and Alaska. Factors associated with seroreactivity to H5Nx HPAI and H5 LPAI were assessed.
Results:The seroprevalence of HPAI among birds in both locations was 7.3% (112/1526). Findings reveal variability in seroprevalence by year, higher rates of exposure to H5 LPAI than H5Nx HPAI overall, and significantly more seropositive and suggestive exposure of birds to H5Nx HPAI in Alaska as compared to Iceland. Geographic, demographic, and taxonomic differences contribute to exposure risk between Alaska and Iceland. Most tested birds were immuno-naïve to HPAI in both locations, which indicates many migratory birds in the subarctic are susceptible to HPAI infection, demonstrating substantial risk for intercontinental transmission between Asia, Europe, and North America.
Conclusions: Our findings provide further justification for increased viral and serosurveillance in Alaska and Iceland to monitor subarctic movements of migratory birds and intercontinental transmission dynamics of currently circulating and new strains of HPAI globally.
Supplementation with Aspergillus oryzae decreases intestinal and fecal methane emissions and affects the production performance of beef cattle
Authors: Zhang Hongrui , Sun Kaijia , Fu Tong , Zhang Liyang , Lian Hongxia , Li Gaiying , Gao Tengyun 
Published: July 2024

Abstract

This study aimed to assess the impact of Aspergillus oryzae supplementation on CH4 emissions and the production performance of beef cattle. Sixteen healthy Simmental crossbred steers (552.38 ± 35.48 kg) were randomly assigned to either a control group (CG, basal diet) or an A. oryzae group (AO, basal diet + 6 g A. oryzae per head daily). CH4 emissions from enteric fermentation and manure, production performance, nutrient and energy digestibility, rumen fermentation parameters, and microbial populations were evaluated. The results showed that A. oryzae supplementation did not significantly affect average daily gain (ADG) or dry matter intake (DMI), though ADG increased by 11.11%. The AO group exhibited a 36.41% increase in apparent NDF digestibility (P < 0.05), a significant reduction in ammonia-N (P < 0.05), and elevated rumen fungi and Butyrivibrio fibrisolvens populations while reducing protozoa and methanogens; CH4 emissions from enteric fermentation and manure decreased by 18.78% (P < 0.05) and 56.55%, respectively. In summary, supplementation with A. oryzae effectively lowers CH4 emissions both enteric fermentation and manure without compromising beef cattle production performance.
Outcome analysis of pulsed radiofrequency for cervical facet joint pain: Can we predict it with sagittal alignment parameters ?
Authors: Yen Cheng-Yo , Lin Sheng-Min , Chen Hong Yu , Wang Shih-Wei , Tsai Yu-Duan , Chye Cien-Leong , Chen Te-Yuan , Wang Hao-Kuang , Wang Kuo-Wei 
Published: July 2024

Abstract

Background
Neck pain due to cervical facet joint pain has a prevalence of 36–60% in chronic neck pain. Pulsed radiofrequency for such pain has been verified. After proper patient selection, pulsed radiofrequency of the cervical facet joints provide long-term pain relief in a routine clinical setting.
Objective
We analyzed the factors from the clinical data and sagittal alignment parameters and investigated the predictors of pulsed radiofrequency for cervical facet joint pain
Methods
There were 204 patients with cervical facet joint pain who received a medial branch block and pulsed radiofrequency between 2015 and 2020. The patients were classified into good and poor outcome groups based on the improvement of the pain score. Clinical and radiological data were analyzed.
Results
Multivariable logistic model showed that the predictors were cervical lordosis including two methods, ossification of the nuchal ligament, number of facet joints, anterior cervical discectomy with fusion, and adjacent facet joint after anterior cervical discectomy with fusion.
Conclusion
With the results, we demonstrated that the outcome were related to cervical lordosis, formation of ossification of nuchal ligament, the number of facet joints, post anterior cervical discectomy with fusion, and adjacent facet joint post anterior cervical discectomy with fusion .
The value of preoperative puncture pain assessment in predicting pain levels after total hip arthroplasty
Authors: Li Bin , Xu Kaige , Guo Tianyu , Jiang Yaping , Wang Yingzhen , Li Tao 
Published: July 2024

Abstract

Background
Total hip arthroplasty (THA) has provided reliable benefits for patients with end-stage degenerative osteoarthritis (OA) of the hip, specifically relief of hip pain, functional recovery, and overall quality of life improvement.Postoperative pain after THA is a frequently reported adverse effect, and prevention of postoperative pain after arthroplasty is particularly important in the treatment program.
Methods
We performed visual analogue scale (VAS) scores by preoperative puncture stimulation, relied on the scores for grouping, compared the changes in VAS scores in the resting and exercise states after surgery. Ninety-eight patients who were admitted to the Affiliated Hospital of Qingdao University from November 2023 to March 2024 for primary THA in our hospital were selected. The patients were grouped based on the pain of puncture during preoperative blood collection, and the VAS scores at rest and exercise were recorded at 1d, 2d, and 3d postoperatively. P < 0.05 indicates statistical significance.
Results
Postoperative VAS scores differed between patients with different preoperative VAS scores. Patients with low preoperative VAS scores had low postoperative VAS scores. Postoperative resting pain VAS scores at 1d, 2d, and 3d were increased in patients with preoperative scores of 3 and 4 compared with the cohort of patients with score of 1, and the difference was statistically significant (p < 0.05). The dynamic pain VAS scores of subgroup 5 were higher than those of subgroup 1 and subgroup 2 at 1d, 2d postoperatively, and the difference was statistically significant (p < 0.05), but the difference was not significant and not statistically significant at 3d.
Conclusions
Patients with low preoperative VAS scores will also have low postoperative VAS scores, and preoperative assessment of patients' VAS scores can predict postoperative VAS scores to some extent.
Effect of sage extract as a natural antioxidant on shear bond strength of metal orthodontic brackets bonded to bleached teeth: An in vitro study.
Authors: Zaino Nagham , Rajeh Nada , Khazem Mays 
Published: July 2024

Abstract

Background
The purpose of this study was to evaluate the effect of sage extract, a natural antioxidant, on the shear bond strength (SBS) of metal orthodontic brackets bonded to bleached enamel with 40% hydrogen peroxide (HP).
Methods and Materials
The sample consisted of 100 maxillary premolars, which were divided into five groups: group I (control, no bleaching), group II (bleaching using HP40% + immediate bonding after bleaching), group III (bleaching using HP40% + SA10% + bracket bonding), group IV (bleaching using HP40% + sage extract 10% + bracket bonding) and group V (bleaching using HP40% + sage extract 20% + bracket bonding). All teeth were bonded using composite resin cement and immersed in distilled water for 24 hours. The shear bond strength (SBS) was assessed by a universal testing machine (Instron) after 24 hours of bracket bonding. After debonding the brackets, the residual adhesive remnant index (ARI) was checked using a stereoscope at 20x magnification. The statistical analysis was carried out using one-way ANOVA to determine differences among the five groups and the least significant difference (LSD) test for bilateral comparisons of the average shear bond strength of the brackets. For the ARI, the Kruskal‒Wallis test was performed.
Results
ANOVA revealed statistically significant differences among the five groups (р<0.001). Compared with those in the bleached group without antioxidant treatment, the shear bond strengths of the groups treated with 10% and 20% sage extracts significantly improved. According to the LSD test, there was no significant difference between the groups treated with 10% and 20% sage extracts (р>0.05). The Kruskal‒Wallis test revealed no statistically significant differences between the frequencies of the residual adhesive remnant index (ARI) among the five study groups, with the most frequent score being (2).
Conclusion
The shear bond strength of brackets bonded to bleached teeth can be improved by applying 10% and 20% sage extracts immediately after bleaching and before bracket bonding.
Development of genogroup-specific ELISA based on the VP1 protein to detect antibodies to GIV and GVI feline norovirus
Authors: Takano Tomomi , Tsuhama Hideyuki , Matsumoto Yuka , Doki Tomoyoshi , Kusuhara Hajime 
Published: July 2024

Abstract

Feline norovirus (FNoV) is a potential pathogen of feline gastroenteritis and has two gene groups (GVI and GVI). Fewer epidemiological studies have been conducted on FNoV. We designed two ELISAs to identify genogroup-specific FNoV antibodies for serological surveillance. Analysis of sera from cats experimentally infected with GIV/GVI FNoV and SPF cats confirmed that the two recombinant proteins react in a genogroup specific manner. Among 183 samples, GIV FNoV and GVI FNoV antibody-positive rates were 6.6 and 26.2%, respectively. The FNoV antibodies of both genogroups were detected in sera collected in 2005, seven years before the presence of FNoV was reported.
Investigation of the formation and variability of dissolved inorganic carbon and dissolved organic carbon in the water of a small river (on the example of the Styr River, Ukraine)
Authors: Biedunkova Olha , Kuznietsov Pavlo 
Published: July 2024

Abstract

This paper presents the results of a study on the dynamics in the concentrations of dissolved inorganic carbon (DIC) and dissolved organic carbon (DOC) in water samples collected from the Styr River between 2019 and 2022. The concentrations of DIC and DOC were measured using an Elementar liqui TOC II analyzer. The study methodology involved analyzing the changes in DIC and DOC concentrations and their relationship with flow rates, temperature, seasonality, and other indicators such as hydrogen pH levels, total alkalinity (TA), and total dissolved solids (TDS). The purpose of this article is to identify patterns in the formation and changes of DIC and DOC concentrations in the Styr River. The concentrations of DIC and DOC in the samples ranged from 1.55-4.93 mM and 0.49-1.43 mM, respectively, with DOC accounting for an average of 22% of the total dissolved carbon content. The highest DOC concentrations were observed in summer, while the highest DIC concentrations were observed in winter. Based on the results, it can be concluded that water flow and temperature have an impact on DOC concentration, while flow, temperature, and pH affect DIC concentration. There was no correlation between DIC and DOC concentrations, but a strong positive relationship (r=0.9056, p<0.001) was found between DIC and TA concentrations. Therefore, the main factors influencing DIC in the Styr River are those that affect the carbonate equilibrium, such as leaching of carbonate and silicate rocks, CO2 absorption from the atmosphere, and changes in pH. Additionally, the concentration of DOC is influenced by biological activity and is higher during the warm season. These findings can be used to develop a strategy for managing water resources in the Styr River basin and to assess and predict the ecological state of the river.