bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Antibrowning Agents on Inhibition of Potato Browning, Volatile Organic Compound Profile, and Microbial Inhibition
Authors: Mosneaguta Ruslan , Alvarez Valente , Barringer Sheryl A. 
Published: October 2012

Abstract:  Burbank and Norkotah potato slices were dipped into 3% sodium acid sulfate (SAS), citric acid (CA), sodium erythorbate (SE), malic acid (MA), sodium acid pyrophosphate (SAPP), or a combination of SAS‐CA‐SE. Browning by polyphenol oxidase (PPO) obtained from potato extract with 0.04 to 0.016 g/mL of antibrowning solutions at pH 2.0 to 6.9 were measured by UV‐Vis spectroscopy. The color of slices dipped in antibrowning solutions at pHs 2 to 7 and stored at 4 °C for 15 d was measured every 5 d by colorimeter. Headspace analysis of volatiles in raw and cooked potato samples was performed by selected ion flow tube mass spectrometer (SIFT‐MS) and soft independent modelling by class analogy (SIMCA) analysis of the calculated odor activity values (OAV) determined interclass distances. Microbial growth was measured at 15 d. At unadjusted pHs (1.1 to 7.1), the PPO browning of the control and samples with SAPP was not significantly different, SAS, CA, and MA produced some inhibition and SE and SAS‐CA‐SE prevented browning. At pH 5 to 7, only SE and SAS‐CA‐SE were effective browning inhibitors. Based on the color of potato slices, SE was the most effective at pH 2 to 7, but SAS was most effective at unadjusted pH. Cooking increased volatile levels in the treated potatoes and decreased differences between volatile profiles. Differences between cooked samples may not be noticeable by the consumer because volatiles with high discriminating powers have low OAVs. SAS, CA, and SAS‐CA‐SE treatments inhibited microbial growth but SAPP, control, and SE did not, most likely due to pH.Practical Application:  Antibrowning agents inhibit polyphenol oxidase, increasing shelf life and consumer acceptability of processed raw potato products by preserving the color. Their effectiveness was shown to be mainly due to a pH effect, except SE, which was not pH dependent. MA, CA, and SAS‐CA‐SE are better acidulants for inhibition of color change as well as growth of spoilage bacteria, yeast, and mold than SAPP, the industry standard.
Survival of Lactobacillus rhamnosus GG as Influenced by Storage Conditions and Product Matrixes
Authors: Klu Yaa Asantewaa Kafui , Williams Jonathan H. , Phillips Robert D. , Chen Jinru 
Published: October 2012

Abstract:  Mortality resulting from diarrhea especially that occurs in children younger than 5 y of age ranks 3rd among all deaths caused by infectious diseases worldwide. Probiotics such as Lactobacillus rhamnosus GG are clinically shown to effectively reduce the incidence of diarrhea in children. A food substrate is one of the major factors regulating the colonization of microorganisms in human gastrointestinal tracts. Peanut butter is a nutritious, low‐moisture food that could be a carrier for probiotics. In this study, we observed the influence of storage conditions and product matrixes on the survival of L. rhamnosus GG. Cells of L. rhamnosus GG were inoculated into full fat or reduced fat peanut butter at 107 CFU/g. Inoculated peanut butter was stored at 4, 25, or 37 °C for 48 wk. Samples were drawn periodically to determine the populations of L. rhamnosus GG. Results showed that there was no significant decrease in the viable counts of L. rhamnosus GG in products stored 4 °C. The survivability of L. rhamnosus GG decreased with increasing storage temperature and time. Product matrixes did not significantly affect the survival of L. rhamnosus GG except at 37 °C. Populations of L. rhamnosus GG were preserved at >6 logs in products stored at 4 °C for 48 wk and at 25 °C for 23 to 27 wk. At 37 °C, the 6‐log level could not be maintained for even 6 wk. The results suggest that peanut butter stored at 4 and 25 °C could serve as vehicles to deliver probiotics.Practical Application:  Lactobacillus rhamnosus GG is clinically shown to effectively reduce the incidence of diarrhea in children. A food substrate is one of the major factors regulating the colonization of microorganisms in human gastrointestinal tracts. Peanut butter is a nutritious, low‐moisture food that could be a carrier for probiotics. In this study, we demonstrated the influence of storage conditions and product matrixes on the survival of L. rhamnosus GG. Results suggest that peanut butters could be effective delivery systems for probiotics and that probitic peanut butter could be used as one of the strategies to control diarrhea and malnutrition in developing countries.
Activation of the insulin‐like growth factor‐1 receptor alters p27 regulation by the epidermal growth factor receptor in oral squamous carcinoma cells
Authors: Jameson Mark J. , Taniguchi Linnea E. , VanKoevering Kyle K. , Stuart Menachem M. , Francom Christian R. , Mendez Rolando E. , Beckler Andrew D. , Carlson Hans T. , Thomas Christopher Y. , Khalil Ashraf A. 
Published: October 2012

BackgroundAlthough oral squamous cell carcinomas (OSCCs) commonly overexpress the epidermal growth factor receptor (EGFR), EGFR tyrosine kinase inhibitors (TKIs) exhibit poor efficacy clinically. Activation of the insulin‐like growth factor‐1 receptor (IGF1R) induces resistance of OSCC cells to EGFR‐TKIs in vitro. This study seeks to evaluate the changes in cell cycle status in OSCC cells in response to gefitinib and IGF1R activation.MethodsSCC‐25 OSCC cells were used for in vitro analyses.ResultsGefitinib caused a 50% reduction in S‐phase population, and IGF1R activation caused a 2.8‐fold increase; combined treatment yielded a baseline S‐phase population. Gefitinib treatment increased the cyclin‐dependent kinase inhibitor p27, and this was not abrogated by IGF1R activation. pT157‐p27 was noted by immunoblot to be decreased on gefitinib treatment, but this was reversed with IGF1R activation. T157 phosphorylation contributes to cytoplasmic localization of p27 where it can promote cell proliferation and cell motility. Using both subcellular fractionation and immunofluorescence microscopy techniques, IGF1R stimulation was noted to increase the relative cytoplasmic localization of p27; this persisted when combined with gefitinib.ConclusionsIGF1R activation partially reverses the cell cycle arrest caused by gefitinib in OSCC cells. While IGF1R stimulation does not eliminate the gefitinib‐induced increase in total p27, its phosphorylation state and subcellular localization are altered. This may contribute to the ability of the IGF1R to rescue OSCC cells from EGFR‐TKI treatment and may have important implications for the use of p27 as a biomarker of cell cycle arrest and response to therapy.
Brain magnetic resonance in experimental acute‐on‐chronic liver failure
Authors: Chavarria Laia , Oria Marc , Romero‐Giménez Jordi , Alonso Juli , Lope‐Piedrafita Silvia , Cordoba Juan 
Published: October 2012

AbstractBackground & AimAcute‐on‐chronic liver failure is the term that refers to sustained liver injury with acute decompensation, usually induced by a precipitating factor. A common link between ensuing failures of various organs is impairment of the vascular supply, which may also induce vasogenic oedema in the brain. The aim of this study was to perform magnetic resonance (MR) study of the brain in a rat model combining bile duct ligation (BDL) and lipopolysaccharide (LPS) administration to investigate brain oedema in liver failure.MethodsBile duct‐ligated rats underwent in vivo brain MR imaging at 4, 5 and 6 weeks, and after superimposed administration of LPS. The MR techniques applied enabled assessment of brain metabolites, and intra‐ or extracellular water distribution. Brain water content was assessed by gravimetry.ResultsMR spectroscopy showed an increase in brain glutamine and a decrease in myo‐inositol and choline in relation to progression of liver disease. BDL rats showed a slight, progressive increase in the amount of cortical brain water that was significant after LPS injection. These changes did not modify the apparent diffusion coefficient, supporting a mixed origin of brain oedema (vasogenic and cytotoxic).ConclusionsThe mechanisms leading to the development of brain oedema in an experimental liver disease model were related to the time course of liver failure and to pro‐inflammatory stimuli. MR findings support the presence of cytotoxic and vasogenic mechanisms in induced brain oedema in BDL rats exposed to LPS.
Draft Genome Sequence of CBS 2479, the Standard Type Strain of Trichosporon asahii
Authors: Yang Rong Ya , Li Hai Tao , Zhu He , Zhou Guang Peng , Wang Meng , Wang Lei 
Published: October 2012

ABSTRACT

Trichosporon asahii
is one of the important opportunistic pathogenic fungi. Here, we first report the draft nuclear chromosome genome sequence and mitochondrial genome sequence of
T. asahii
CBS 2479, which is a standard strain of
T. asahii
that was isolated from a progressive psoriatic lesion. COG analysis predicted that 3,131 genes were assigned to 23 functional categories and that 628 genes were predicted to have a general function.
Complete Mitochondrial and Plastid Genomes of the Green Microalga Trebouxiophyceae sp. Strain MX-AZ01 Isolated from a Highly Acidic Geothermal Lake
Authors: Servín-Garcidueñas Luis E. , Martínez-Romero Esperanza 
Published: October 2012

ABSTRACT

We report the complete organelle genome sequences of
Trebouxiophyceae
sp. strain MX-AZ01, an acidophilic green microalga isolated from a geothermal field in Mexico. This eukaryote has the remarkable ability to thrive in a particular shallow lake with emerging hot springs at the bottom, extremely low pH, and toxic heavy metal concentrations.
Trebouxiophyceae
sp. MX-AZ01 represents one of few described photosynthetic eukaryotes living in such a hostile environment. The organelle genomes of
Trebouxiophyceae
sp. MX-AZ01 are remarkable. The plastid genome sequence currently presents the highest G+C content for a trebouxiophyte. The mitochondrial genome sequence is the largest reported to date for the
Trebouxiophyceae
class of green algae. The analysis of the genome sequences presented here provides insight into the evolution of organelle genomes of trebouxiophytes and green algae.
Genome Sequence of Staphylococcus aureus Newbould 305, a Strain Associated with Mild Bovine Mastitis
Authors: Bouchard Damien , Peton Vincent , Almeida Sintia , Le Maréchal Caroline , Miyoshi Anderson , Azevedo Vasco , Berkova Nadia , Rault Lucie , François Patrice , Schrenzel Jacques , Even Sergine , Hernandez David , Le Loir Yves 
Published: October 2012

ABSTRACT

Staphylococcus aureus
is a major etiological agent of mastitis in ruminants. We report here the genome sequence of bovine strain Newbould 305, isolated in the 1950s in a case of bovine mastitis and now used as a model strain able to reproducibly induce chronic mastitis in cows.
Draft Genome Sequence of Virgibacillus halodenitrificans 1806
Authors: Lee Sang-Jae , Lee Yong-Jik , Jeong Haeyoung , Lee Sang Jun , Lee Han-Seung , Pan Jae-Gu , Kim Byoung-Chan , Lee Dong-Woo 
Published: October 2012

ABSTRACT

Virgibacillus halodenitrificans
1806 is an endospore-forming halophilic bacterium isolated from salterns in Korea. Here, we report the draft genome sequence of
V. halodenitrificans
1806, which may reveal the molecular basis of osmoadaptation and insights into carbon and anaerobic metabolism in moderate halophiles.
Genome Sequence of the Thermophile Bacillus coagulans Hammer, the Type Strain of the Species
Authors: Su Fei , Tao Fei , Tang Hongzhi , Xu Ping 
Published: October 2012

ABSTRACT

Here we announce a 3.0-Mb assembly of the
Bacillus coagulans
Hammer strain, which is the type strain of the species within the genus
Bacillus
. Genomic analyses based on the sequence may provide insights into the phylogeny of the species and help to elucidate characteristics of the poorly studied strains of
Bacillus coagulans
.
Complete Genome Sequences of Desulfosporosinus orientis DSM765T, Desulfosporosinus youngiae DSM17734T, Desulfosporosinus meridiei DSM13257T, and Desulfosporosinus acidiphilus DSM22704T
Authors: Pester Michael , Brambilla Evelyne , Alazard Didier , Rattei Thomas , Weinmaier Thomas , Han James , Lucas Susan , Lapidus Alla , Cheng Jan-Fang , Goodwin Lynne , Pitluck Sam , Peters Lin , Ovchinnikova Galina , Teshima Hazuki , Detter John C. , Han Cliff S. , Tapia Roxanne , Land Miriam L. , Hauser Loren , Kyrpides Nikos C. , Ivanova Natalia N. , Pagani Ioanna , Huntmann Marcel , Wei Chia-Lin , Davenport Karen W. , Daligault Hajnalka , Chain Patrick S. G. , Chen Amy , Mavromatis Konstantinos , Markowitz Victor , Szeto Ernest , Mikhailova Natalia , Pati Amrita , Wagner Michael , Woyke Tanja , Ollivier Bernard , Klenk Hans-Peter , Spring Stefan , Loy Alexander 
Published: October 2012

ABSTRACTDesulfosporosinusspecies are sulfate-reducing bacteria belonging to theFirmicutes. Their genomes will give insights into the genetic repertoire and evolution of sulfate reducers typically thriving in terrestrial environments and able to degrade toluene (Desulfosporosinus youngiae), to reduce Fe(III) (Desulfosporosinus meridiei,Desulfosporosinus orientis), and to grow under acidic conditions (Desulfosporosinus acidiphilus).
Complete Genome Sequence of Mycobacterium fortuitum subsp. fortuitum Type Strain DSM46621
Authors: Ho Yung S. , Adroub Sabir A. , Aleisa Fajr , Mahmood Hanan , Othoum Ghofran , Rashid Fahad , Zaher Manal , Ali Shahjahan , Bitter Wilbert , Pain Arnab , Abdallah Abdallah M. 
Published: October 2012

ABSTRACT

Mycobacterium fortuitum
is a member of the rapidly growing nontuberculous mycobacteria (NTM). It is ubiquitous in water and soil habitats, including hospital environments.
M. fortuitum
is increasingly recognized as an opportunistic nosocomial pathogen causing disseminated infection. Here we report the genome sequence of
M. fortuitum
subsp.
fortuitum
type strain DSM46621.
Complete Genome Sequence of Bacillus subtilis Strain QB928, a Strain Widely Used in B. subtilis Genetic Studies
Authors: Yu Chi-Shing , Yim Kay-Yuen , Tsui Stephen Kwok-Wing , Chan Ting-Fung 
Published: October 2012

ABSTRACT

The complete genome sequence of
Bacillus subtilis
strain QB928 was constructed to facilitate studies in the evolution of the genetic code. With a widespread use of the strain in
Bacillus subtilis
genetics studies, its complete genome sequence would facilitate deeper understanding of
Bacillus subtilis
genetics.
Draft Genome Sequence of a Nonhemolytic Fish-Pathogenic Streptococcus agalactiae Strain
Authors: Delannoy Christian M. J. , Zadoks Ruth N. , Lainson Frederick A. , Ferguson Hugh W. , Crumlish Margaret , Turnbull James F. , Fontaine Michael C. 
Published: October 2012

ABSTRACT

Streptococcus agalactiae
is a significant Gram-positive bacterial pathogen of terrestrial and aquatic animals. A subpopulation of nonhemolytic strains which appear to be pathogenic only for poikilotherms exists. We report here the first draft genome sequence of a nonhemolytic
S. agalactiae
isolate recovered from a diseased fish.
Genome Sequence of the Swine Pathogen Streptococcus suis Serotype 2 Strain S735
Authors: Boyle Brian , Vaillancourt Katy , Bonifait Laetitia , Charette Steve J. , Gottschalk Marcelo , Grenier Daniel 
Published: October 2012

ABSTRACT

Streptococcus suis
is a major swine pathogen responsible for significant, worldwide economic losses in the swine industry, in addition to being an emerging zoonotic agent. Strains of serotype 2 are the most commonly associated with infections causing meningitis, endocarditis, and septicemia. Here we present the genome sequence of
S. suis
serotype 2 strain S735.
Whole-Genome Sequence of
N
-Acylhomoserine Lactone-Synthesizing and -Degrading Acinetobacter sp. Strain GG2
Authors: Hong Kar-Wai , Koh Chong-Lek , Sam Choon-Kook , Yin Wai-Fong , Chan Kok-Gan 
Published: October 2012

ABSTRACT

Acinetobacter
sp. strain GG2 is a quorum-sensing and quorum-quenching bacterium isolated from the ginger rhizosphere. It degrades a broad range of
N
-acylhomoserine lactone molecules via lactonase. The genome sequence of strain GG2 may provide insights on the regulation of quorum-sensing and quorum-quenching mechanisms in this bacterium.
Genome Sequence of Pectobacterium carotovorum subsp. carotovorum Strain PCC21, a Pathogen Causing Soft Rot in Chinese Cabbage
Authors: Park Tae-Ho , Choi Beom-Soon , Choi Ah-Young , Choi Ik-Young , Heu Sunggi , Park Beom-Seok 
Published: October 2012

ABSTRACT

Pectobacterium carotovorum
is a plant-pathogenic enterobacterium responsible for soft rot in various commercially important plants. Here we report the complete genome sequence and automatic annotation of strain PCC21.
Draft Genome Sequence of Salmonella enterica Serovar Typhimurium ST1660/06, a Multidrug-Resistant Clinical Strain Isolated from a Diarrheic Patient
Authors: Li Lei , Cheng Chi Keung , Cheung Man Kit , Law Patrick Tik Wan , Ling Julia Mei Lun , Kam Kai Man , Cheung William Man Wai , Kwan Hoi Shan 
Published: October 2012

ABSTRACT

Salmonella enterica
serovar Typhimurium is one of the most prevalent serovars of
Salmonella
that causes human gastroenteritis. Here, we report the draft genome sequence of the
S
. Typhimurium multidrug-resistant strain ST1660/06. Comparative genomic analysis unveiled three strain-specific genomic islands that potentially confer the multidrug resistance and virulence of the strain.
Genome Sequence of Dyella japonica Strain A8, a Quorum-Quenching Bacterium That Degrades
N
-Acylhomoserine Lactones, Isolated from Malaysian Tropical Soil
Authors: Chen Jian-Woon , Chan Kok-Gan 
Published: October 2012

ABSTRACT

Dyella japonica
strain A8 is a Malaysian tropical soil bacterial strain which shows
N
-acylhomoserine lactone-degrading activity. Here, we present its draft genome sequence. A putative quorum-quenching gene was identified based on the genome sequence analysis of strain A8. To the best of our knowledge, this is the first genome announcement of a member from the genus of
Dyella
, and this is also the first work that reports the quorum-quenching activity of
Dyella japonica
.
Draft Genome Sequence of an Aeromonas sp. Strain 159 Clinical Isolate That Shows Quorum-Sensing Activity
Authors: Chan Xin Yue , Chua Kek Heng , Puthucheary Savithri D. , Yin Wai-Fong , Chan Kok-Gan 
Published: October 2012

ABSTRACT

Aeromonas
is a pathogenic organism that is often found to infect humans. Here we report the draft genome of a clinical isolate in Malaysia,
Aeromonas
sp. strain 159, which shows
N
-acylhomoserine lactone production. In the draft genome of strain 159,
luxI
and
luxR
homologue genes were found to be located at contig 47, and these genes are believed to be important for the quorum-sensing system present in this pathogen.
Genome Sequence of the Halotolerant Staphylococcus sp. Strain OJ82, Isolated from Korean Traditional Salt-Fermented Seafood
Authors: Sung Jung-Suk , Chun Jongsik , Choi Sungjong , Park Woojun 
Published: October 2012

ABSTRACT

Staphylococcus
sp. strain OJ82 was isolated from a Korean traditional fermented squid seafood, ojingeo-jeotgal.
Staphylococcus
sp. OJ82 could grow and show extracellular protease and β-galactosidase activities in the presence of extremely high saline (20%). Here, we report the genome sequence of
Staphylococcus
sp. OJ82.
Genome Sequence of Paenibacillus alvei DSM 29, a Secondary Invader during European Foulbrood Outbreaks
Authors: Djukic Marvin , Becker Dominik , Poehlein Anja , Voget Sonja , Daniel Rolf 
Published: October 2012

ABSTRACT

Paenibacillus alvei
is known as a secondary invader during European foulbrood of honeybees. Here, we announce the 6.83-Mb draft genome sequence of
P. alvei
type strain DSM 29. Putative genes encoding an antimicrobial peptide, a binary toxin, a mosquitocidal toxin, alveolysin, and different polyketides and nonribosomal peptides were identified.
An evaluation of a new prehospital pre-alert guidance tool
Authors: Booth Sean Michael , Bloch Mark 
Published: October 2012

BackgroundThe requirement for guidance regarding ambulance crews pre-alerting patients into hospital emergency departments (ED) has been well established, but a clear guidance tool that supports a decision to pre-alert a receiving hospital is lacking.AimsTo investigate the impact of a new pre-alert tool on current alerting practice and evaluate its ability to take the place of a prehospital early warning system.MethodsData were collected for a sample of patients brought by ambulance to the resuscitation area of Aberdeen Royal Infirmary ED over a 7-week period. Basic demographic information plus alert status and guidance prompt status was collected and compared with a pragmatic alert requirement. Analysis of ambulance crew alert decisions and the pre-alert guidance prompt advice was undertaken and compared.ResultsAmbulance crew decisions to alert had a sensitivity of 72% (CI 62% to 80%), specificity of 50% (CI 27% to 73%), positive predictive value (PPV) of 90% and negative predictive value (NPV) of 22%. The pre-alert guidance alert prompt had a sensitivity of 99% (CI 94% to 100%), specificity of 64% (CI 39% to 84%), PPV of 95% and NPV of 90%. 28% of patients were under-alerted by ambulance crews, mostly medical patients presenting with chest pain.ConclusionsThe pre-alert guidance tool shows face validity and superior ability to advise a pre-alert than ambulance crew decisions. It supplements a practitioners’ clinical decision-making and has been regarded as having a positive impact on ED triage and utilisation of resources. Further levels of validity are expected to be achieved with continued audit and ongoing use of this tool.
Bed-sharing and risk of hospitalisation due to pneumonia and diarrhoea in infancy: the 2004 Pelotas Birth Cohort
Authors: Ngale Kátia M A , Santos Iná S , González-Chica David A , de Barros Aluísio J D , Matijasevich Alicia 
Published: October 2012

ObjectiveTo investigate the association between bed-sharing with the mother at 3 months of age and incidence of hospitalisation due to pneumonia and diarrhoea between 3 and 12 months.MethodsThe 2004 Pelotas Birth Cohort included all live births to mothers living in Pelotas, Brazil, in 2004. Information on bed-sharing was obtained at the 3-month follow-up visit, and on hospitalisations at the 12-month visit, both based on mothers’ reports. Only singleton infants with complete information on hospitalisation were analysed.Results3906 infants were included. The bed-sharing prevalence at 3 months was 46.4% (95% CI 44.9 to 48.0%). The incidence of pneumonia admissions between 3 and 12 months was 3.6% (95% CI 3.3 to 4.2%) and diarrhoea, 0.9% (95% CI 0.6 to 1.2%). In crude analyses, bed-sharing with the mother was associated with higher incidence of hospitalisation due to both pneumonia and diarrhoea. There was interaction between bed-sharing and duration of breastfeeding regarding the chance of admission due to pneumonia. Among infants breastfed for 3 months or less, the chance of hospitalisation due to pneumonia among bed-sharers was almost twice as high as among non-bed-sharers (adjusted OR 1.96; 95% CI 1.08 to 3.55). There was no association between bed-sharing and hospitalisation due to pneumonia among infants breastfed for longer than 3 months in crude or adjusted analyses. The association between bed-sharing and admissions due to diarrhoea lost statistical significance after allowing for confounders.ConclusionsThe effect of bed-sharing in infancy on the risk of hospitalisation due to pneumonia depends on breastfeeding, such that weaned children present higher risk.