bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of inward leakage of full-face masks in EN and ISO standards: comparison of gas and aerosol test agents
Authors: Santandrea Audrey , Marchal Mathieu , Chazelet Sandrine , Marsteau Stéphanie 
Published: July 2024

Abstract
In Europe, respiratory protective devices must be certified before they can be marketed. Among the parameters of interest, inward leakage (IL) characterizes the tightness between the face seal and the face, to verify that the device is well-designed. European standard EN 13274-1 (2001) and International Organization for Standardization (ISO) standard ISO 16900-1 (2019) specify that IL should be measured using sodium chloride (NaCl) aerosol or sulfur hexafluoride (SF6) gas. For reusable masks made of nonporous materials, both test agents are considered equally acceptable. However, the few studies that have compared IL values measured with various aerosols and gases have come to divergent conclusions. This work then aimed to measure IL with the test agents recommended by the standards to determine whether they are really equivalent. Since krypton (Kr) is an interesting candidate for replacing SF6 in standard tests, IL was assessed with SF6 and Kr simultaneously, and with NaCl aerosol using various calculation methods.
Tests were carried out on 5 models of full-face masks donned on a headform connected to a breathing machine simulating 3 sinusoidal breathing rates of various intensities. The respirator fit on the headform was evaluated using a controlled negative pressure method to determine a manikin fit factor. Four scenarios were then tested to represent very poor, bad, good, and excellent fit. Gas concentration was measured using a mass spectrometer, and IL was calculated for SF6 and Kr. A combination of 3 devices allowed the determination of the number-based concentration of particles with diameters between 20 nm and 2 µm, and IL was calculated for each of the 33 channels, as well as using a cumulative number concentration. In addition, to comply with standards, a conversion was carried out to calculate IL using a cumulative mass concentration.
The results of this work evidenced that the IL values measured with NaCl were systematically lower than those determined with gases. IL was also shown to vary with particle size, with a maximum value exceeding that calculated with cumulative concentrations (in number or mass). As part of the revision of the standards, protocols for measuring inward leakage should be redefined. On the one hand, acceptability thresholds should be re-evaluated according to the nature of the test agent (gas or aerosol), as it is clear that the 2 options do not give the same results for a given configuration. On the other hand, the aerosol leakage measurement protocol needs to be reworked to enable the measurement of a well-defined, robust, and reproducible inward leakage value.
The Epistemic Goals of the Humanities
Authors: Grimm Stephen R 
Published: July 2024

Abstract
The sciences aim to get at the truth about the nature of the world. Do the humanities have a similar goal—namely, to get at the truth about things like novels, paintings, and historical events? I consider a few different ways in which the humanities aim at the truth about their objects, in the process giving rise to epistemic goods such as knowledge and understanding. Two works in the humanities are used as test cases: the historian Tyler Stovall’sParis Noir (1996) and the musicologist Susan McClary’s article, ‘The Blasphemy of Talking Politics During Bach Year’ (1987).
Illumination Fading
Authors: Martin M G F 
Published: July 2024

Abstract
Bertrand Russell abandoned the notion of acquaintance in July 1918. What changes does this force in his account of the mind? This paper focuses on one puzzle of interpretation about this. In 1913, Russell offered an account of ‘egocentric particulars’, his term for indexicals and demonstratives. He argued that the fundamental objection to neutral monism was that it could not provide an adequate theory of these terms. In 1918, Russell now embraces a form of neutral monism, but he does not return to the problem of indexicals until 1940 in his William James lectures. Is the account given in 1940 significantly different from the one given in 1913? What was the argument against neutral monism in 1913? Does Russell offer a new solution in 1940 or reject his earlier view as mistaken? The answers offered here are used to draw more general morals about the current debate concerning relational theories of sense perception.
Liberation Philosophy
Authors: Cassam Quassim 
Published: July 2024

Abstract
Liberation philosophy seeks to contribute to the liberation of the oppressed and to the creation of a more just society. A meliorative philosophy is one that improves human lives. A liberation philosophy can be regarded as meliorative only if it has a compelling theory of change. A theory of change for philosophical interventions should explain how they can contribute to social, political or economic change. The main components of such a theory are identified and shown to be present in the work of the best liberation philosophers, such as Martin Luther King Jr. A meliorative philosophy improves human lives by, among other things, providing the kind of guidance that leads to better decision-making and improved conduct. Philosophy should conceive of the guidance it offers as co-created and reflect on the conditions for effective co-creation. The distinctive virtues of meliorative philosophy, including liberation philosophy, are personal qualities that enable co-creation. These include humility, practicality, an openness to diverse perspectives, and an instinct for lived complexity. There are philosophical purists who reject the demand that philosophy should answer to practical needs. We should be sceptical about some of the claims made by philosophical purists.
A novel copy number variant in the murine Cdh23 gene gives rise to profound deafness and vestibular dysfunction
Authors: Boehler Nicholas A , Seheult Shane D I , Wahid Muhammad , Hase Kazuma , D’Amico Sierra F , Saini Shakshi , Mascarenhas Brittany , Bergman Matthew E , Phillips Michael A , Faure Paul A , Cheng Hai-Ying Mary 
Published: July 2024

Abstract
Hearing loss is the most common congenital sensory deficit worldwide and exhibits high genetic heterogeneity, making molecular diagnoses elusive for most individuals. Detecting novel mutations that contribute to hearing loss is crucial to providing accurate personalized diagnoses, tailored interventions, and improving prognosis. Copy number variants (CNVs) are structural mutations that are understudied, potential contributors to hearing loss. Here, we present the Abnormal Wobbly Gait (AWG) mouse, the first documented mutant exhibiting waltzer-like locomotor dysfunction, hyperactivity, circling behaviour, and profound deafness caused by a spontaneous CNV deletion in cadherin 23 (Cdh23). We were unable to identify the causative mutation through a conventional whole-genome sequencing (WGS) and variant detection pipeline, but instead found a linked variant in hexokinase 1 (Hk1) that was insufficient to recapitulate the AWG phenotype when introduced into C57BL/6J mice using CRISPR-Cas9. Investigating nearby deafness-associated genes revealed a pronounced downregulation of Cdh23 mRNA and a complete absence of full-length CDH23 protein, which is critical for the development and maintenance of inner ear hair cells, in whole head extracts from AWG neonates. Manual inspection of WGS read depth plots of the Cdh23 locus revealed a putative 10.4 kb genomic deletion of exons 11 and 12 that was validated by PCR and Sanger sequencing. This study underscores the imperative to refine variant detection strategies to permit identification of pathogenic CNVs easily missed by conventional variant calling to enhance diagnostic precision and ultimately improve clinical outcomes for individuals with genetically heterogenous disorders such as hearing loss.
Neurodevelopmental disorder-associated CYFIP2 regulates membraneless organelles and eIF2α phosphorylation via protein interactors and actin cytoskeleton
Authors: Zhang Yinhua , Kang Hyae Rim , Jun Yukyung , Kang Hyojin , Bang Geul , Ma Ruiying , Ju Sungjin , Yoon Da Eun , Kim Yoonhee , Kim Kyoungmi , Kim Jin Young , Han Kihoon 
Published: July 2024

Abstract
De novo variants in the Cytoplasmic FMR1-interacting protein 2 (CYFIP2) have been repeatedly associated with neurodevelopmental disorders and epilepsy, underscoring its critical role in brain development and function. While CYFIP2’s role in regulating actin polymerization as part of the WAVE regulatory complex (WRC) is well-established, its additional molecular functions remain relatively unexplored. In this study, we performed unbiased quantitative proteomic analysis, revealing 278 differentially expressed proteins (DEPs) in the forebrain of Cyfip2 knock-out embryonic mice compared to wild-type mice. Unexpectedly, these DEPs, in conjunction with previously identified CYFIP2 brain interactors, included not only other WRC components but also numerous proteins associated with membraneless organelles (MLOs) involved in mRNA processing and translation within cells, including the nucleolus, stress granules, and processing bodies. Additionally, single-cell transcriptomic analysis of the Cyfip2 knock-out forebrain revealed gene expression changes linked to cellular stress responses and MLOs. We also observed morphological changes in MLOs in Cyfip2 knock-out brains and CYFIP2 knock-down cells under basal and stress conditions. Lastly, we demonstrated that CYFIP2 knock-down in cells, potentially through WRC-dependent actin regulation, suppressed the phosphorylation levels of the alpha subunit of eukaryotic translation initiation factor 2 (eIF2α), thereby enhancing protein synthesis. These results suggest a physical and functional connection between CYFIP2 and various MLO proteins and also extend CYFIP2’s role within the WRC from actin regulation to influencing eIF2α phosphorylation and protein synthesis. With these dual functions, CYFIP2 may fine-tune the balance between MLO formation/dynamics and protein synthesis, a crucial aspect of proper mRNA processing and translation.
Small molecules, enormous functions: potential approach for overcoming bottlenecks in embryogenic tissue induction and maintenance in conifers
Authors: Guo Tao , Bao Fen , Fan Yingming , Zhang Jinfeng , Zhao Jian 
Published: July 2024

Abstract
Somatic embryogenesis (SE) is not only the most effective method among various strategies for the asexual propagation of forest trees but also a basis for genetic improvement. However, some bottlenecks, such as the recalcitrance of initiation, the maintenance of embryogenic potential during proliferation and the low efficiency of maturation as well as high rate of abnormal embryo development remain unresolved. These bottlenecks refer to complex mechanisms, including transcriptional regulatory networks, epigenetic modifications and physiological conditions. In recent years, several small molecules utilized in animal stem cell research have exhibited positive effects on plant regeneration, including conifer species, which offers a potential novel approach to overcome the challenges associated with SE in conifers. In this review, we summarize the small molecules used in conifers, including redox substances, epigenetic regulatory inhibitors and other metabolism-related molecules, which overcome these difficulties without the use of genetic engineering. Moreover, this approach also has the advantages of dynamic reversibility, simple operation, and simultaneous regulation of multiple targets, which might be one of the best choices for optimizing plant regeneration systems including SE.
Grapevine gray mold disease: infection, defense and management
Authors: Rahman Mati Ur , Liu Xia , Wang Xiping , Fan Ben 
Published: July 2024

Abstract
Grapevine (Vitis vinifera L.,) is among the world’s leading fruit crops. The production of grapes is severely affected by many diseases including gray mold, caused by the necrotrophic fungus Botrytis cinerea. Although all Vitis species can be hosts for B. cinerea, V. vinifera are particularly susceptible. Accordingly, this disease poses a significant threat to the grape industry and causes substantial economic losses. Development of resistant V. vinifera cultivars has progressed from incidental selection by farmers, to targeted selection through the use of statistics and experimental design, to the employment of genetic and genomic data. Emerging technologies such as marker-assisted selection and genetic engineering have facilitated the development of cultivars that possess resistance to B. cinerea. A promising method involves using the CRISPR/Cas9 system to induce targeted mutagenesis and develop genetically modified non-transgenic crops. Hence, scientists are now engaged in the active pursuit of identifying genes associated with susceptibility and resistance. This review focuses on the known mechanisms of interaction between the B. cinerea pathogen and its grapevine host. It also explores innate immune systems that have evolved in V. vinifera, with the objective of facilitating the rapid development of resistant grapevine cultivars.
The crisis in the palm of our hand
Authors: Norman Jethro , Ford Matthew , Cold-Ravnkilde Signe Marie 
Published: July 2024

Abstract
The rapid global proliferation of smartphones and their associated information infrastructures has been a defining feature of the past decade's global crises. Yet, while the digital is now a topic of keen interest for scholars working on virtually everything that constitutes the international, the smartphone as an object of study in and of itself has been largely elusive. Moreover, emerging studies of contemporary crisis, such as ‘polycrisis’, often downplay the role of the digital. How can we conceptualize the ambiguity and ubiquity of the smartphone, as it impacts diverse fields of human action, from war to humanitarianism to democracy? And how can we empirically study this phenomenon and its distributed effects? We contend that smartphones are both embedded in and embed global crises. We conceptualize this as ‘global crisis ecologies’: new spaces that are not simply geographical, or easily framed in terms of North/South divisions, and that include the informational infrastructures that mediate the way crisis is apprehended. This framing helps us understand how multiple civilian, state and non-state actors at different societal levels participate in crises through everyday smartphone use. It foregrounds how the speed, audio-visual capabilities and inherent scalability of smartphones shape how crises are perceived and managed.
Platform kinship and the reshaping of political order in the Somali territories
Authors: Norman Jethro 
Published: July 2024

Abstract
Numerous studies show that digital technologies facilitate diaspora engagement in homeland affairs. However, communities in home countries also adapt digital platforms to harness diasporic support and drive socio-political change. Despite a rich literature on ‘digital kinship’, there remains a limited understanding of kinship's broader political and developmental impact, especially in (post)conflict regions. This article draws on fieldwork in the Somali territories to argue that a distinctive model of governance, platform kinship, is emerging as an alternative to existing state and international development programmes. Focusing on WhatsApp, it highlights how the platform's specific features are adapted to Somali segmentary clan structures, enabling kinship groups to bridge digital divides, preserve oral traditions and uphold egalitarian principles. Platform kinship has state-like effects. Through an ecosystem of WhatsApp groups, geographically dispersed kin mediate disputes, coordinate development projects, fund political campaigns, and respond to conflicts and crises. However, it also empowers new actors, including youth, politicians and business elites, while marginalizing elders. Furthermore, because platform kinship strengthens the clan as the central political unit, it can deepen divisions between kinship groups and undermine state-building projects and conceptions of national identity. This has important implications for policy-makers and academics working on digital governance, development and peacebuilding.
The political economy of connectivity in the Somali Horn of Africa
Authors: Iazzolino Gianluca , Musa Ahmed M 
Published: July 2024

Abstract
This article focuses on the political economy of connectivity in the Somali Horn of Africa (HoA). On the one hand, it charts the emergence of firms in Somalia and Somaliland at the intersection of telecommunication and finance, eventually controlling logistics through digital platforms that enable the circulation of people, goods and value across the region. On the other, it examines the historical and political elements that have shaped a peculiar instance of digital capitalism against a backdrop of protracted insecurity and state fragility. It uses the specific case-study of digital platforms in the Somali HoA to provide insights into the multiple shapes that digital capitalism can take. To advance the authors' analysis, the article links the notions of digital infrastructural power and ‘extrastatecraft’. Empirically, it delves into the case of digital platforms in the Somali HoA to illustrate how the unique digital landscape of the region has evolved over fifty years of political instability and economic volatility. Theoretically, the article navigates the implications of the state's heavy reliance on privately owned and managed platforms amid the backdrop of variegated digital capitalism narratives, which have predominantly centred on the global North.
Piracy and the broader ‘gun business’ in the Niger Delta
Authors: Jacobsen Katja Lindskov , Rasmussen Amanda Møller 
Published: July 2024

Abstract
Piracy cannot be understood in isolation from other (criminal) engagements, but how can we explore such linkages and why is it relevant to understand their implications, both for piracy and counterpiracy? Appreciating that Niger Delta-based piracy has dropped to historically low levels, this article explores what pirates do when they do not sustain themselves by engaging in piracy. As such, the article offers novel empirical insights into underexplored dimensions of piracy. Moreover, we use this case-study to demonstrate the relevance of an infrastructure-inspired conceptualization much beyond analyses of piracy and counterpiracy. Showing how piracy is linked to broader and often violent infrastructures that interventions leave unaddressed, and stressing key implications of the continuity and change in such linkages, is a first step towards appreciating the centrality of a broader dilemma that intervention actors confront: how to conceptualize the necessity to delimit a problem, while appreciating the significance of problem-dimensions left unaddressed? Our suggestion is that an infrastructure-inspired approach offers a helpful starting-point, a suggestion that we demonstrate in an analysis of how Niger Delta-based piracy forms part of a broader ‘gun business’ landscape, which does not only entail different types of crime but also various types of victims.
From Individual Calcifiers to Ecosystem Dynamics: Ocean Acidification Effects on Urchins and Abalone
Authors: deVries Maya S , Ly Nhi , Ebner Chase , Hallisey Ryan 
Published: July 2024

Synopsis
A central question in ecology is to what extent do trophic interactions govern the structure and function of communities? This question is becoming more pressing as trophic interactions shift with rapid climate change. Sea urchins and abalone are key invertebrates in the habitats where they reside. Sea urchins are critical members of exemplar trophic cascades in kelp forests due to their impact on kelp establishment and maintenance; yet their populations are controlled by predators, such as sea otters and sunflower sea stars. Abalone compete with urchins for macroalgal food resources and therefore can help regulate urchin populations in kelp forests. Given that both urchin tests and abalone shells used for predator defense are comprised of calcium carbonate, much research has been conducted on the impacts of ocean acidification (OA) on these calcified structures. A growing body of literature has shown that urchin tests are less calcified and break with less force under OA conditions. Less is known about abalone, but their shells also appear to respond negatively to OA. Using kelp forest communities as exemplar ecosystems, we discuss the morphological, biomechanical, and physiological responses to OA in urchins and abalone and consider how these individual level responses scale to trophic interactions and ultimately whole ecosystem processes. Although the impacts of OA on the calcified structures used for defense have been well studied, calcified mechanisms for food consumption, such as the Aristotle's lantern of urchins, are much less understood. Thus, examining both the feeding and defense sides of trophic interactions would greatly improve our understanding of OA responses across individual to ecosystem scales. More generally, measurements of morphological, biomechanical, and physiological responses to OA can be made in individuals to help predict higher level ecological responses, which would greatly contribute to broader predictions of whole ecosystem responses to OA.
Pharmacokinetics and pharmacological target attainment of standard temocillin dosing in non-critically ill patients with complicated urinary tract infections
Authors: Wijnant Gert-Jan , Ngougni Pokem Perrin , Coessens Marie , Cottone Eleonora , Ermtraud Julian , Goeman Lieven , Vervaeke Steven , Wicha Sebastian G , Van Bambeke Françoise 
Published: July 2024

Abstract

Objectives
Temocillin, a carbapenem-sparing β-lactam antibiotic, is commonly used at the standard 4 g/day dosage for treating complicated urinary tract infections (cUTIs). However, pharmacokinetic/pharmacodynamic (PK/PD) data supporting this regimen is limited. This study evaluated the plasma pharmacokinetics (PK) and PTA of temocillin in non-critically ill cUTI patients with varying degrees of renal insufficiency (RI).

Methods
In this single-centre clinical study, 22 cUTI patients received a fixed 4 g/day (2 g q12h, intravenously) temocillin dose, irrespective of renal function (no RI: n = 5, mild RI: n = 8, moderate RI: n = 9). Plasma samples were collected post-dosing for LC-MS analysis of total and unbound temocillin levels. Monte Carlo simulations were performed based on the established PK/PD target of ≥35% fT &gt; MIC (minimal inhibitory concentration).

Results
Among patients, the highest plasma drug exposure and PK/PD target attainment were observed in those with moderate RI (median AUC0–12h = 1143 h.mg/L and %fT &gt; MIC = 68%), followed by mild RI patients (median AUC0–12h = 918 h.mg/L and %fT &gt; MIC = 34%), and the lowest in those with healthy kidney function (median AUC0–12h = 692 h.mg/L and %fT &gt; MIC = 26%). Simulations indicated that the 4 g/day temocillin dose achieves 90% PTA only for glomerular filtration rate &lt; 60 mL/min and MIC ≤ 8 mg/L.

Conclusion
The standard temocillin dose may need to be increased from 4 to 6 g/day to treat non-critically ill cUTI patients, in line with recent EUCAST recommendations. For patients with moderate RI, who experience higher exposure due to reduced renal drug clearance, 4 g/day temocillin remains appropriate.
Less water in agriculture? Potential and challenges in optimizing water use efficiency
Authors: Wientjes Emilie , Seijger Chris 
Published: July 2024

Abstract
This article comments on:
Turc B, Sahay S, Haupt J, de Oliveira Santos T, Bai G, Glowacka K. 2024. Up-regulation of non-photochemical quenching improves water use efficiency and reduces whole-plant water consumption under drought in Nicotianatabacum. Journal of Experimental Botany 75, 3959–3972.