bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipid Droplets: A New Player in Colorectal Cancer Stem Cells Unveiled by Spectroscopic Imaging
Authors: Tirinato Luca , Liberale Carlo , Di Franco Simone , Candeloro Patrizio , Benfante Antonina , La Rocca Rosanna , Potze Lisette , Marotta Roberto , Ruffilli Roberta , Rajamanickam Vijayakumar P. , Malerba Mario , De Angelis Francesco , Falqui Andrea , Carbone Ennio , Todaro Matilde , Medema Jan Paul , Stassi Giorgio , Di Fabrizio Enzo 
Published: September 2014

Abstract
The cancer stem cell (CSC) model is describing tumors as a hierarchical organized system and CSCs are suggested to be responsible for cancer recurrence after therapy. The identification of specific markers of CSCs is therefore of paramount importance. Here, we show that high levels of lipid droplets (LDs) are a distinctive mark of CSCs in colorectal (CR) cancer. This increased lipid content was clearly revealed by label-free Raman spectroscopy and it directly correlates with well-accepted CR-CSC markers as CD133 and Wnt pathway activity. By xenotransplantation experiments, we have finally demonstrated that CR-CSCs overexpressing LDs retain most tumorigenic potential. A relevant conceptual advance in this work is the demonstration that a cellular organelle, the LD, is a signature of CSCs, in addition to molecular markers. A further functional characterization of LDs could lead soon to design new target therapies against CR-CSCs. Stem Cells 2015;33:35–44
Glucose Transporter 1-Positive Endothelial Cells in Infantile Hemangioma Exhibit Features of Facultative Stem Cells
Authors: Huang Lan , Nakayama Hironao , Klagsbrun Michael , Mulliken John B. , Bischoff Joyce 
Published: September 2014

Abstract
Endothelial glucose transporter 1 (GLUT1) is a definitive and diagnostic marker for infantile hemangioma (IH), a vascular tumor of infancy. To date, GLUT1-positive endothelial cells in IH have not been quantified nor directly isolated and studied. We isolated GLUT1-positive and GLUT1-negative endothelial cells from IH specimens and characterized their proliferation, differentiation, and response to propranolol, a first-line therapy for IH, and to rapamycin, an mTOR pathway inhibitor used to treat an increasingly wide array of proliferative disorders. Although freshly isolated GLUT1-positive cells, selected using anti-GLUT1 magnetic beads, expressed endothelial markers CD31, VE-Cadherin, and vascular endothelial growth factor receptor 2, they converted to a mesenchymal phenotype after 3 weeks in culture. In contrast, GLUT1-negative endothelial cells exhibited a stable endothelial phenotype in vitro. GLUT1-selected cells were clonogenic when plated as single cells and could be induced to redifferentiate into endothelial cells, or into pericytes/smooth muscle cells or into adipocytes, indicating a stem cell-like phenotype. These data demonstrate that, although they appear and function in the tumor as bona fide endothelial cells, the GLUT1-positive endothelial cells display properties of facultative stem cells. Pretreatment with rapamycin for 4 days significantly slowed proliferation of GLUT1-selected cells, whereas propranolol pretreatment had no effect. These results reveal for the first time the facultative nature of GLUT1-positive endothelial cells in IH. Stem Cells 2015;33:133–145
Focal Adhesion Kinase and Wnt Signaling Regulate Human Ductal Carcinoma In Situ Stem Cell Activity and Response to Radiotherapy
Authors: Williams Kathryn E. , Bundred Nigel J. , Landberg Göran , Clarke Robert B. , Farnie Gillian 
Published: September 2014

Abstract
Cancer stem cells (CSCs) can avoid or efficiently repair DNA damage from radio and chemotherapy, which suggests they play a role in disease recurrence. Twenty percentage of patients treated with surgery and radiotherapy for ductal carcinoma in situ (DCIS) of the breast recur and our previous data show that high grade DCIS have increased numbers of CSCs. Here, we investigate the role of focal adhesion kinase (FAK) and Wnt pathways in DCIS stem cells and their capacity to survive irradiation. Using DCIS cell lines and patient samples, we demonstrate that CSC-enriched populations are relatively radioresistant and possess high FAK activity. Immunohistochemical studies of active FAK in DCIS tissue show high expression was associated with a shorter median time to recurrence. Treatment with a FAK inhibitor or FAK siRNA in nonadherent and three-dimensional matrigel culture reduced mammosphere formation, and potentiated the effect of 2 Gy irradiation. Moreover, inhibition of FAK in vitro and in vivo decreased self-renewal capacity, levels of Wnt3a and B-Catenin revealing a novel FAK-Wnt axis regulating DCIS stem cell activity. Overall, these data establish that the FAK-Wnt axis is a promising target to eradicate self-renewal capacity and progression of human breast cancers. Stem Cells 2015;33:327–341
Predicting patterns of intentional introduction of non‐native largemouth bass into farm ponds in northeastern Japan
Authors: Tsunoda Hiroshi , Mitsuo Yoshito , Enari Hiroto 
Published: September 2014

AbstractNon‐native species are difficult to eradicate or control once established. Thus, the prevention of invasions is a high priority for conserving native ecosystems. Our objective was to determine the factors that influence illegal introductions of non‐native largemouth bass (Micropterus salmoides) and to develop a model that could be used to predict the pattern of future illegal introduction by anglers. We used generalized linear modeling (GLM) to evaluate the relationship between landscape and environmental factors and the presence of bass in farm ponds. Our results suggest that the occurrence of bass was primarily associated with pond size, distance of ponds from the urban center, and the proportion of the pond shoreline covered with a concrete revetment. We conclude that introductions occurred in ponds that were: easier to locate on a map, closer to anglers’ residential areas, and more easily accessible to the pond shore. Last, we suggest our predictive model could be used for preventive measures, such as identifying other ponds most at risk of future illegal introduction of non‐native bass.
Fabrication and tribological properties of superhydrophobic nickel films with positive and negative biomimetic microtextures
Authors: Wang Ying , Yang Jing , Guo Xiaobao , Zhang Qiang , Wang Jingyu , Ding Jianning , Yuan Ningyi 
Published: September 2014

Abstract
Nickel (Ni) films with positive and negative textured surfaces of lotus and rice leaf patterns were fabricated through an inexpensive and effective method. The as-prepared Ni films were superhydrophobic and exhibited excellent tribological properties after chemical treatment. Experimental results indicated that the water contact angles (WCAs) on the surfaces of biomimetic textured Ni films (approximately 120°) were far greater than those on smooth films (65°). The biomimetic textured surfaces became superhydrophobic (WCA of approximately 150°) after perfluoropolyether (PFPE) treatment, which could be due to the combined effects of the special texture and the PFPE. The as-prepared biomimetic-textured Ni films modified with PFPE were improved with a low friction coefficient and excellent antiwear properties, which were due to the combination of the effective lubrication of PFPE and the special textures that served as a good lubricant and a debris reservoir. Moreover, the antiwear properties of the as-prepared Ni films with negative biomimetic microtextures modified with PFPE were much better than those of films with positive biomimetic microtextures modified with PFPE.
Current therapy of granulomatosis with polyangiitis and microscopic polyangiitis: the role of rituximab
Authors: Geetha Duvuru , Kallenberg Cees , Stone John H. , Salama Alan D. , Appel Gerald B. , Duna George , Brunetta Paul , Jayne David 
Published: September 2014

AbstractGranulomatosis with polyangiitis and microscopic polyangiitis are anti-neutrophil cytoplasmic antibody-associated vasculitides (AAVs) that are prone to cycles of remission and relapse. The introduction of cytotoxic therapy has changed the prognosis for these diseases from typically fatal to manageable chronic illnesses with a relapsing course. Despite improvements in outcomes, recurrence of disease and drug-related toxicity continue to produce significant morbidity and mortality. Better understanding of the pathogenesis of AAV and the mechanism of action of cyclophosphamide has led to investigation of therapies that target B cells. Two randomized controlled trials have shown that rituximab is not inferior to cyclophosphamide for induction of remission in severe AAV, with no significant difference in the incidence of overall adverse events in rituximab- versus cyclophosphamide-treated patients. Data from ongoing clinical trials will determine the role of rituximab in the maintenance of remission.
A decade’s investigation of the stability of erodible stream beds
Authors: Colombini Marco 
Published: September 2014

AbstractThis contribution is a remake of the one published under the same title by Reynolds (Nord. Hydrol., vol. 7, 1976, pp. 161–183). As in that paper, attention is given to the latest developments in the field of river morphodynamics and, in particular, on the formation of bed patterns. Stimulated by the work by Kidanemariam and Uhlmann (J. Fluid Mech., vol. 750, 2014, R2) the opportunity is taken to examine the most recent developments in terms of sediment transport models.
Numerical investigation of the development of three-dimensional wavepackets in a sharp cone boundary layer at Mach 6
Authors: Sivasubramanian Jayahar , Fasel Hermann F. 
Published: September 2014

AbstractDirect numerical simulations were performed to investigate wavepackets in a sharp cone boundary layer at Mach 6. In order to understand the natural transition process in hypersonic cone boundary layers, the flow was forced by a short-duration (localized) pulse. The pulse disturbance developed into a three-dimensional wavepacket, which consisted of a wide range of disturbance frequencies and wavenumbers. First, the linear development of the wavepacket was studied by forcing the flow with a low-amplitude pulse (0.001 % of the free-stream velocity). The dominant waves within the resulting wavepacket were identified as the second-mode axisymmetric disturbance waves. In addition, weaker first-mode oblique disturbance waves were also observed on the lateral sides of the wavepacket. In order to investigate the nonlinear transition regime, large-amplitude pulse disturbances (0.5 % of the free-stream velocity) were introduced. The response of the flow to the large-amplitude pulse disturbances indicated the presence of a fundamental resonance mechanism. Lower secondary peaks in the disturbance wave spectrum were identified at approximately half the frequency of the high-amplitude frequency band, suggesting the possibility of a subharmonic resonance mechanism. However, the spectrum also indicated that the fundamental resonance was much stronger than the subharmonic resonance. A secondary stability investigation using controlled disturbances confirmed that fundamental resonance is indeed a dominant mechanism compared to subharmonic resonance. Furthermore, strong peaks in the disturbance wave spectrum were also observed for low-azimuthal-wavenumber second-mode oblique waves, hinting at a possible oblique breakdown mechanism. Thus, the wavepacket simulations indicate that the second-mode fundamental resonance and oblique breakdown mechanisms are the strongest for the investigated flow. Hence, both mechanisms are likely to be relevant in the natural transition process for a cone boundary layer at Mach 6.
Multidimensional homophily in friendship networks
Authors: BLOCK PER , GRUND THOMAS 
Published: September 2014

AbstractHomophily—the tendency for individuals to associate with similar others—is one of the most persistent findings in social network analysis. Its importance is established along the lines of a multitude of sociologically relevant dimensions, e.g. sex, ethnicity and social class. Existing research, however, mostly focuses on one dimension at a time. But people are inherently multidimensional, have many attributes and are members of multiple groups. In this article, we explore such multidimensionality further in the context of network dynamics. Are friendship ties increasingly likely to emerge and persist when individuals have an increasing number of attributes in common? We analyze eleven friendship networks of adolescents, draw on stochastic actor-oriented network models and focus on the interaction of established homophily effects. Our results indicate that main effects for homophily on various dimensions are positive. At the same time, the interaction of these homophily effects is negative. There seems to be a diminishing effect for having more than one attribute in common. We conclude that studies of homophily and friendship formation need to address such multidimensionality further.
Associative learning for host-induced fruit volatiles in Psyttalia concolor (Hymenoptera: Braconidae), a koinobiont parasitoid of tephritid flies
Authors: Canale A. , Geri S. , Benelli G. 
Published: September 2014

AbstractParasitic wasps are fascinating organisms that rely on a wide range of communication channels to locate their hosts. Associative learning for foraging kairomones has been demonstrated for various parasitic wasps, but little is known for parasitoids of Tephritidae flies. Psyttalia concolor (Hymenoptera: Braconidae) is a koinobiont parasitoid able to attack at least 14 tephritid pests. Females are innately attracted by some host-induced plant volatiles (HIPVs), whereas others of the same bouquet are unattractive. We hypothesize that females may detect unattractive HIPVs in association with key resources, such as food and hosts, learning to respond favourably to these cues in consecutive experiences. We evaluated associative learning for HIPVs in P. concolor females, testing if they are able to associate a food reward with the presence of different dosages of three HIPVs, thus developing a preference for an odour innately unattractive. Results demonstrated that P. concolor responded favourably to the learned cue in consecutive experiences. For all tested HIPVs (nonanoic acid, decanoic acid and geranyl acetone), regardless of dosage, trained females preferred the reward-associated odour, whereas naïve did not. Both HIPV-trained and naïve females did not show consistent differences in latencies when choosing HIPVs over blank. HIPV-trained and naïve wasps did not spend more time on HIPVs over blank. Odour learning is of adaptive importance for this generalist parasitoid, since it enhances host location efficiency by reducing the time wasted on the decision of where to search for hosts. From an applied perspective, these HIPVs could be used to train mass-reared P. concolor in pre-release, to potentially improve its efficacy in the field.
Histopathological and audiological effects of mechanical trauma associated with the placement of an intracochlear electrode, and the benefit of corticosteroid infusion: prospective animal study
Authors: Malkoc G , Dalgic A , Koc M , Kandogan T , Korkmaz S , Ceylan M E , Inan S , Olgun L 
Published: September 2014

AbstractObjective:This study aimed to present the histopathological and audiological effects of mechanical trauma associated with the placement of a model electrode in the scala tympani in rats, and the effects of continuous topical corticosteroid application.Method:The study comprised three groups of rats. The round window membrane was perforated in all three groups and a model electrode was inserted in the round window. Group one received no further treatments. Groups two and three also had an intrathecal microcatheter compatible with a mini-osmotic pump inserted; in group two this was used to release normal saline and in group three the pump released 400 µg/ml dexamethasone.Results:Dexamethasone infusion given after implantation of the intracochlear model electrode was more effective for preventing hearing loss than the administration of just one dose of dexamethasone.Conclusion:The findings suggest that continuous dexamethasone infusion is beneficial for preventing the loss of hair cells and neurons associated with early and late periods of intracochlear electrode trauma.
Bone cement: how effective is it at restoring hearing in isolated incudostapedial erosion?
Authors: Watson G J , Narayan S 
Published: September 2014

AbstractObjective:To determine the effectiveness of biocements in rebridging isolated incudostapedial erosion.Methods:A review of the use of biocements for isolated incudostapedial joint erosion was performed on publications from 1998 to 2012 available from Medline, Embase and Pubmed. Inclusion criteria were papers published in English, case series or comparative studies with more than 10 patients, isolated incudostapedial erosion through chronic ear disease, minimal air-bone gap less than 20 dB (or air-bone gap less than 10 dB) and follow up for at least one year.Results:In 108 patients, rebridging ossiculoplasty was performed using hydroxyapatite cement. Closure of air-bone gaps less than 20 dB and less than 10 dB was achieved in 80–94.4 per cent and 29–75 per cent, respectively. Glass ionomer cement was used in 318 patients. Closure of air-bone gaps less than 20 dB and less than 10 dB was achieved in 74–94 per cent and 40–76 per cent, respectively.Conclusion:Biocements can be successfully used to close isolated incudostapedial erosions. Larger comparative prospective studies documenting the length of eroded incus and types of reformation of the incudostapedial joint, with standardised reporting, are needed in the future.
Guy of Saint-Denis on the tones: thinking about chant for Saint-Denis c.1300
Authors: MEWS CONSTANT J. , CROSSLEY JOHN N. , WILLIAMS CAROL 
Published: September 2014

ABSTRACTThis article examines the thinking of Guy of Saint-Denis about plainchant tones as formulated in his Tractatus de Tonis (c.1300), preserved as the final item in an anthology of texts that he prepared (British Library, MS Harley 281). It examines his attitude to each of the major theorists singled out in this anthology. It argues that Guy's approach to chant combines the practically oriented writings of Guido of Arezzo with the Aristotelian perspective formulated by Johannes de Grocheio, but takes that perspective a step further by reflecting on the ways different types of chant impact on the emotions. Guy was also much influenced by Peter of Auvergne, a philosopher in the Arts Faculty at Paris committed to developing the teaching of Thomas Aquinas. Careful corrections to the Tractatus in Harley 281 reflect this ongoing concern to refine his thinking, possibly stimulated by Jerome of Moravia. His core conviction is that chant modes each have an affective attribute, and need to be chosen according to the subject matter of the text being sung. Guy criticised the practice of choosing modes sequentially in liturgical offices composed by those he calls ‘moderns’. Guy argues his case by drawing on examples of chant from Saint-Denis. A case can be made, on palaeographic grounds, for identifying him with Guy of Châtres, abbot of Saint-Denis (1326–42) and author of a Sanctilogium that updates the traditional monastic martyrology by reference to more recent Dominican collections of saints' lives in order to make them more accessible for liturgical use.
Poetic measures of architecture: Martin Heidegger’s ‘…Poetically Man Dwells…’
Authors: Hill Glen 
Published: September 2014

Martin Heidegger begins his lecture ‘… Poetically Man Dwells …’ by denying poetry is a marginal practice whose imaginings are ‘mere fancies and illusions’. ‘[T]he poetic’, he states, is not ‘merely an ornament and bonus added on to dwelling’. On the contrary, Heidegger boldly claims that poetry is the source of all human dwelling on earth: ‘[…] poetry first causes dwelling to be dwelling. Poetry is what really lets us dwell.’The connective tissue of Heidegger's argument in ‘… Poetically Man Dwells …’ is the concept of ‘measure’. In the English translation of the lecture, permutations of the term ‘measure’ (Maß/messen) appear a remarkable ninety-four times, not including dozens more uses of its synonyms: ‘dimension’, ‘span’, ‘meter’ and ‘gauge’. What seems surprising, given that the set-up of the lecture revolves around poetry and measure, is that the commonest understanding of measure related to poetry – poetic measure itself – is not discussed thematically by Heidegger. Rather, Heidegger's incessant word play produces meanings that include ‘measuring against’ in the sense of comparing to a standard, ‘measuring up’ a space by ‘stepping-out’ (durchmessen), ‘measuring out’ in the sense of dividing-up or apportioning (das Zu-Gemessene), ‘being measured’ in the sense of having propriety, ‘taking measures’ (die Maß-Nahme) in response to a situation, and ‘measuring between’ as a distance or span. These meanings are of course related to common poetic measure, and might even be claimed to be its ground.
Towards Practical Application of Paper based Printed Circuits: Capillarity Effectively Enhances Conductivity of the Thermoplastic Electrically Conductive Adhesives
Authors: Wu Haoyi , Chiang Sum Wai , Lin Wei , Yang Cheng , Li Zhuo , Liu Jingping , Cui Xiaoya , Kang Feiyu , Wong Ching Ping 
Published: September 2014

AbstractDirect printing nanoparticle-based conductive inks onto paper substrates has encountered difficulties e.g. the nanoparticles are prone to penetrate into the pores of the paper and become partially segmented and the necessary low-temperature-sintering process is harmful to the dimension-stability of paper. Here we prototyped the paper-based circuit substrate in combination with printed thermoplastic electrically conductive adhesives (ECA), which takes the advantage of the capillarity of paper and thus both the conductivity and mechanical robustness of the printed circuitsweredrastically improved without sintering process. For instance, the electrical resistivity of the ECA specimen on a pulp paper (6 × 10−5Ω·cm, with 50 wt% loading of Ag) was only 14% of that on PET film than that on PET film. This improvement has been found directly related to the sizing degree of paper, in agreement with the effective medium approximation simulation results in this work. The thermoplastic nature also enables excellent mechanical strength of the printed ECA to resist repeated folding. Considering the generality of the process and the wide acceptance of ECA technique in the modern electronic packages, this method may find vast applications in e.g. circuit boards, capacitive touch pads and radio frequency identification antennas, which have been prototyped in the manuscript.
Mountain runoff vulnerability to increased evapotranspiration with vegetation expansion
Authors: Goulden Michael L. , Bales Roger C. 
Published: September 2014

Significance
Climate change has the potential to reduce the supply of surface water by accelerating mountain vegetation growth and evapotranspiration (ET), though the likelihood and severity of this effect are poorly known. We used the upper Kings River basin in California’s Sierra Nevada as a case study of the sensitivity of runoff to increased ET with warming. We found that Kings River flow is highly sensitive to vegetation expansion; warming projected for 2100 could increase ET across the Kings River watershed by 28% and decrease riverflow by 26%. Moreover, we found a consistent relationship between watershed ET and temperature across the Sierra Nevada; this consistency implies a potential widespread reduction in water supply with warming, with important implications for California’s economy and environment.