bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of physical activity on surrogate markers of cardiovascular disease in the haemodialysis population
Authors: Hull Katherine L , Abell Lucy , Adenwalla Sherna F , Billany Roseanne E , Burns Stephanie , Burton James O , Churchward Darren , Graham-Brown Matthew P M , Gray Laura J , Highton Patrick , Lightfoot Courtney J , Said Rahma , Smith Alice C , Young Hannah M L , March Daniel S 
Published: June 2024

ABSTRACT

Background
The haemodialysis (HD) population is sedentary, with substantial cardiovascular disease risk. In the general population, small increases in daily step count associate with significant reductions in cardiovascular mortality. This study explores the relationship between daily step count and surrogate markers of cardiovascular disease, including left ventricular ejection fraction (LVEF) and native T1 (a marker of diffuse myocardial fibrosis), within the HD population.

Methods
This was a post hoc analysis of the association between daily step count and metabolic equivalent of task (MET) and prognostically important cardiac magnetic resonance imaging parameters from the CYCLE-HD study (ISRCTN11299707). Unadjusted linear regression and multiple linear regression adjusted for age, body mass index, dialysis vintage, haemoglobin, hypertension and ultrafiltration volume were performed. Significant relationships were explored with natural cubic spline models with four degrees of freedom (five knots).

Results
A total of 107 participants were included [age 56.3 ± 14.1 years, 79 (73.8%) males]. The median daily step count was 2558 (interquartile range 1054–4352). There were significant associations between steps and LVEF (β = 0.292; P = .009) and steps and native T1 (β = −0.245; P = .035). Further modelling demonstrated most of the increase in LVEF occurred at up to 2000 steps/day and there was an inverse dose–response relationship between steps and native T1, with the most pronounced reduction in native T1 between ≈2500 and 6000 steps/day.

Conclusions
The results suggest an association between daily step count and parameters of cardiovascular health in the HD population. These findings support the recommendations for encouraging physical activity but are not the justification. Further research should evaluate whether a simple physical activity intervention improves cardiovascular outcomes in individuals receiving maintenance HD.
Early Intestinal Ultrasound in Severe Ulcerative Colitis Identifies Patients at Increased Risk of 1-Year Treatment Failure and Colectomy
Authors: Ilvemark Johan F K F , Wilkens Rune , Thielsen Peter , Dige Anders , Boysen Trine , Brynskov Jørn , Bjerrum Jacob T , Seidelin Jakob B 
Published: June 2024

Abstract

Background and Aims
Reliable and easily accessible objective markers of disease activity to predict long-term treatment outcomes in severe ulcerative colitis [UC] are missing. We aimed to investigate if intestinal ultrasound [IUS] might predict long-term outcomes in hospitalised patients with severe UC, treated with intravenous [IV] corticosteroids.

Methods
Hospitalised patients with severe UC and IUS inflammation (bowel wall thickness [BWT] &gt; 3.0 mm) starting IV corticosteroids were recruited at three university hospitals in Denmark. IUS was performed before treatment and 48 ± 24 h, 6 ± 1 days, and 3 months after treatment initiation. Time until colectomy or need for new interventions was registered together with Mayo score at 3 months and partial Mayo score [pMayo] at 12 months. Follow-up time was 12 months.

Results
In the final analysis, 56 patients were included; 45 [80%] patients needed intervention, including nine colectomies, during the 12-month follow-up. After 48 ± 24 h, no patient with a BWT &lt; 3 mm needed a colectomy, p = 0.04. BWT ≥ 4 mm showed an increased risk of colectomy {odds ratio 9.5 (95% confidence interval [CI] 1.5–186), p = 0.03}, whereas a BWT ≥ 3 mm showed an increased risk of intervention (3.6 [1.1–12.5], p = 0.03). A BWT ≥ 4 mm resulted in a significantly shorter time until both colectomy, p = 0.03, and treatment intensification (mean days 75 [95% CI 24–127] vs 176 [119–233], p = 0.005). However, neither IUS parameters nor pMayo score, C-reactive protein [CRP], haemoglobin, or p-albumin could predict remission at 3 and 12 months.

Conclusion
BWT, assessed at 48 h post intravenous corticosteroid initiation in patients hospitalised with severe UC, may identify patients with an increased risk of short- and long-term colectomy and predict a more aggressive short-term disease course.
Analysis of genetic and clinical characteristics of androgen insensitivity syndrome: a cohort study including 12 families
Authors: Yuan Zheng , Fan Lijun , Wang Yi , Li Lele , Ren Xiaoya , Sui Shengbin , Song Yanning , Cheng Ming , Cao Bingyan , Gong Chunxiu 
Published: June 2024

Abstract

Context
Androgen insensitivity syndrome (AIS) manifests itself as variable symptoms of under-virilization in patients with 46,XY disorders caused by androgen receptor (AR) gene variants. This large-sample study aimed to correlate the genotypes and phenotypes to the fertility of individuals.

Methods
This was a cohort study that analyzed the genetic and clinical characteristics of patients with AIS from a single center in China.

Results
The 117 patients were divided into 53 with complete AIS (CAIS) and 64 with partial AIS (PAIS). At their first visit, the median age was 1.83 years (0.92-4.17), and the external masculinization score was 3.0 (2.0-6.0). At the last follow-up, 92% (49/53) of patients with CAIS maintained their female gender, and 94% (60/64) of patients with PAIS were raised as males. No gender anxiety was observed in this study. Eighty-eight AR variants were identified, with 31 (35%) being unreported. Moreover, 24% (21/88) occurred more than once. The variants that appeared most frequently were located at amino acid 841, including p.R841H (n = 5) and p.R841C (n = 2). Variants p.N706S, p.R856H, and p.A871V were each observed 4 times. In terms of inheritance, 83% of patients with parental verification inherited variants from their mothers. We also observed that the variants from 1 case were inherited from his maternal grandfather who had hypospadias.

Conclusion
Most children with PAIS were raised as males. The abundance of maternally inheritable variants and the presence of case of preserved fertility indicate the fertility potential in patients with AIS. Hence, we recommend a careful evaluation of gonadectomy when fertility preservation is being considered.
Life history traits and cancer prevalence in birds
Authors: Kapsetaki Stefania E , Compton Zachary T , Dolan Jordyn , Harris Valerie Κ , Mellon Walker , Rupp Shawn M , Duke Elizabeth G , Harrison Tara M , Aksoy Selin , Giraudeau Mathieu , Vincze Orsolya , McGraw Kevin J , Aktipis Athena , Tollis Marc , Boddy Amy Μ , Maley Carlo C 
Published: June 2024

ABSTRACT

Background and objectives
Cancer is a disease that affects nearly all multicellular life, including the broad and diverse taxa of Aves. While little is known about the factors that contribute to cancer risk across Aves, life history trade-offs may explain some of this variability in cancer prevalence. We predict birds with high investment in reproduction may have a higher likelihood of developing cancer. In this study, we tested whether life history traits are associated with cancer prevalence in 108 species of birds.

Methodology
We obtained life history data from published databases and cancer data from 5,729 necropsies from 108 species of birds across 24 taxonomic orders from 25 different zoological facilities. We performed phylogenetically controlled regression analyses between adult body mass, lifespan, incubation length, clutch size, sexually dimorphic traits, and both neoplasia and malignancy prevalence. We also compared the neoplasia and malignancy prevalence of female and male birds.

Results
Providing support for a life history trade-off between somatic maintenance and reproduction, we found a positive relationship between clutch size and cancer prevalence across Aves. There was no significant association with body mass, lifespan, incubation length, sexual dimorphism, and cancer.

Conclusions and implications
Life history theory presents an important framework for understanding differences in cancer defenses across various species. These results suggest a trade-off between reproduction and somatic maintenance, where Aves with small clutch sizes get less cancer.
Changes to circulating tumor cells in the central vein during laparoscopic versus transanal endoscopic surgeries for rectal cancer: can surgical approach make a difference?
Authors: Chen Mian , Ye Fujin , Zheng Wenwen , Xiong Li , Liang Zhenxing , Liu Huashan , Zheng Xiaobin , Li Wenxin , Kang Liang , Huang Liang 
Published: June 2024

Abstract

Background
The oncological safety of transanal total mesorectal excision (taTME) remains uncertain, and its special surgical approach may contribute to tumor cell dissemination. Thus, we conducted a study to investigate the impact of surgical approach on circulating tumor cell (CTC) counts and phenotypes in rectal cancer.

Methods
This is a prospective randomized controlled study (ClinicalTrials: NCT05109130). The patients were randomized to either the taTME (n = 49) or laparoscopic TME (laTME) (n = 48) groups. Blood samples were collected from the central vein to measure CTC counts and phenotypes at three time points: preoperative (t1), immediately post-tumor removal (t2), and one week post-surgery (t3). The effect of surgical procedure on CTCs at each time point was analyzed, with the primary endpoint being the change in CTC counts from t1 to t3 for each surgical approach. This study adheres to Consolidated Standards of Reporting Trials Guidelines.

Results
The baseline clinicopathologic characteristics of the laTME and taTME groups were balanced. The change in CTC count from t1 to t3 was 1.81 ± 5.66 in the laTME group and 2.18 ± 5.53 in the taTME group. The taTME surgery was non-inferior to laTME in terms of changing CTC counts (mean difference [MD]: −0.371; 95% confidence interval [CI]: −2.626 to 1.883, upper-sided 95% CI of 1.883 &lt; 2, non-inferiority boundary value). Compared with that at t1, the CTC count at t2 did not change significantly. However, higher CTC counts were detected at t3 than at t2 in the taTME (P = 0.032) and laTME (P = 0.003) groups. From t1 to t3, CTC counts significantly increased in both the taTME (P = 0.008) and laTME (P = 0.031) groups. There were no significant differences in CTC phenotype changes between the two groups from t1 to t3.

Conclusions
Compared with laTME, taTME did not affect CTC counts and phenotypes. Our findings indicate that taTME is not inferior to laTME in terms of CTC changes from an oncological perspective.
DNA Transposons Favor De Novo Transcript Emergence Through Enrichment of Transcription Factor Binding Motifs
Authors: Lebherz Marie Kristin , Fouks Bertrand , Schmidt Julian , Bornberg-Bauer Erich , Grandchamp Anna 
Published: June 2024

Abstract
De novo genes emerge from noncoding regions of genomes via succession of mutations. Among others, such mutations activate transcription and create a new open reading frame (ORF). Although the mechanisms underlying ORF emergence are well documented, relatively little is known about the mechanisms enabling new transcription events. Yet, in many species a continuum between absent and very prominent transcription has been reported for essentially all regions of the genome. In this study, we searched for de novo transcripts by using newly assembled genomes and transcriptomes of seven inbred lines of Drosophila melanogaster, originating from six European and one African population. This setup allowed us to detect sample specific de novo transcripts, and compare them to their homologous nontranscribed regions in other samples, as well as genic and intergenic control sequences. We studied the association with transposable elements (TEs) and the enrichment of transcription factor motifs upstream of de novo emerged transcripts and compared them with regulatory elements. We found that de novo transcripts overlap with TEs more often than expected by chance. The emergence of new transcripts correlates with regions of high guanine-cytosine content and TE expression. Moreover, upstream regions of de novo transcripts are highly enriched with regulatory motifs. Such motifs are more enriched in new transcripts overlapping with TEs, particularly DNA TEs, and are more conserved upstream de novo transcripts than upstream their ‘nontranscribed homologs’. Overall, our study demonstrates that TE insertion is important for transcript emergence, partly by introducing new regulatory motifs from DNA TE families.
Long-Read–Based Hybrid Genome Assembly and Annotation of Snow Algal Strain CCCryo 101-99 (cf. Sphaerocystis sp., Chlamydomonadales)
Authors: Çiftçi Ozan , Zervas Athanasios , Lutz Stefanie , Feord Helen , Keusching Christoph , Leya Thomas , Tranter Martyn , Anesio Alexandre M , Benning Liane G 
Published: June 2024

Abstract
Polar regions harbor a diversity of cold-adapted (cryophilic) algae, which can be categorized into psychrophilic (obligate cryophilic) and cryotrophic (nonobligate cryophilic) snow algae. Both can accumulate significant biomasses on glacier and snow habitats and play major roles in global climate dynamics. Despite their significance, genomic studies on these organisms remain scarce, hindering our understanding of their evolutionary history and adaptive mechanisms in the face of climate change. Here, we present the draft genome assembly and annotation of the psychrophilic snow algal strain CCCryo 101-99 (cf. Sphaerocystis sp.). The draft haploid genome assembly is 122.5 Mb in length and is represented by 664 contigs with an N50 of 0.86 Mb, a Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness of 92.9% (n = 1,519), a maximum contig length of 5.3 Mb, and a guanine-cystosine (GC) content of 53.1%. In total, 28.98% of the genome (35.5 Mb) contains repetitive elements. We identified 417 noncoding RNAs and annotated the chloroplast genome. The predicted proteome comprises 14,805 genes with a BUSCO completeness of 97.8%. Our preliminary analyses reveal a genome with a higher repeat content compared with mesophilic chlorophyte relatives, alongside enrichment in gene families associated with photosynthesis and flagella functions. Our current data will facilitate future comparative studies, improving our understanding of the likely response of polar algae to a warming climate as well as their evolutionary trajectories in permanently cold environments.
Misinformation surrounding sodium reduction for blood pressure: content analysis of Japanese posts on X
Authors: Terada Marina , Okuhara Tsuyoshi , Nishiie Yuriko , Kiuchi Takahiro , Murakami Kentaro 
Published: June 2024

Abstract
This content analysis aimed to assess misinformation themes regarding sodium reduction and blood pressure on X with the goal of providing strategies to address and debunk such misinformation. A total of 531 posts were manually coded into sodium-related misinformation themes, with inclusion criteria for posts asserting no association between sodium reduction and hypertension or claiming consuming sodium is beneficial for health. Numbers and post frequencies per misinformation theme were calculated. Post characteristics, including information sources, advertisements and narratives, were coded, and a correlation analysis was conducted to assess their association with each misinformation theme. Fourteen sodium-related misinformation themes were identified and consistently disseminated on X. The predominant theme, ‘Natural Salt’, accounted for 37.7% (n = 200), reaching 1.6 million followers, followed by ‘Reducing salt could be bad for my health’ theme, comprising 28.6% (n = 152) and reaching 1.5 million followers. There was a statistical correlation between the natural salt misinformation theme and advertisements. Many of the most frequent misinformation themes identified in this study have not been systematically debunked by organizations such as the World Health Organization and the American Heart Association. This study underscores the importance of continuous monitoring and analysis of sodium-related misinformation on social media platforms and their underlying commercial interests. Such monitoring has the potential to identify prevalent misinformation themes that may pose harm to the public and to inform public health organizations, enabling them to proactively address potential issues through debunking.
IBD Is Like a Tree: Reflections From Older Adults With Inflammatory Bowel Disease
Authors: Rusher Alison , Araka Elizabeth , Ananthakrishnan Ashwin N , Ritchie Christine , Kochar Bharati 
Published: June 2024

Abstract

Background
Despite the growing proportion of older adults with inflammatory bowel disease (IBD), their lived experience is not well understood. IBD literature is generally focused on younger adults, and few studies are qualitative. Older adults may report well-being differently than younger adults, so it is important that we learn about their goals and priorities with a chronic disease.

Objective
The study sought to understand the lived experience of older adults with IBD and explore their perceptions and priorities.

Methods
We conducted in-depth interviews with patients ≥60 years of age with IBD to evaluate the impact and perception of IBD in the context their overall health and life. We used a hybrid inductive-deductive thematic analysis of our transcripts to identify underlying patterns.

Results
We achieved thematic saturation after 22 interviews. We produced 4 major themes: (1) having IBD at an older age, (2) financial ramifications of IBD at an older age, (3) expectations for a meaningful life, and (4) unmet needs. Prominent subthemes included (1) ageism, loss of autonomy, and barriers to healthcare; (2) retirement and insurance issues; (3) redefining quality of life and gratitude; and (4) social isolation and navigating daily life with IBD.

Conclusions
Having IBD later in life presents unique challenges. Physicians treating older patients should consider age-sensitive communication, susceptibility to social isolation, and practices for healthy aging in the context of IBD. Patient priorities for further investigation include more representation in the media and educational material tailored for older adults with IBD.
When to Go Against the Flow: Examining Patterns of Performance Over Multiday Migration Events in the Hawaiian Stream Fish, ‘O‘opu Nōpili (Sicyopterus stimpsoni)
Authors: Diamond Kelly M , Nishiura Lance , Sakihara Troy , Schoenfuss Heiko L , Blob Richard W 
Published: June 2024

Synopsis
Many animals migrate across regions of their geographic range as part of extended events, with groups of individuals proceeding through areas of travel on several successive days. Early migrating individuals may have an advantage over late migrating individuals by gaining early access to the resources at the eventual destination. For situations where early access to resources would provide an advantage, specific sets of locomotor traits might be found among individuals that are earlier migrators. We tested for associations between migration timing and traits related to escape responses, climbing, and morphology in the amphidromous Hawaiian stream goby, ‘o‘opu nōpili (Sicyopterus stimpsoni). In this species, juvenile fish migrate in pulses over several days immediately following flash floods. We collected daily measurements of escape responses and waterfall climbing from juvenile fish arriving at streams from the ocean. We found that escape performance showed mainly stochastic variation across migrating individuals tested on successive days. In contrast, some metrics of climbing performance decrease over successive pulses during a migration event. We also found more variation in body shape among fish from early pulses during migration events compared to later in pulses. These results could have implications for guiding conservation efforts, identifying critical time windows for protection as periods with the greatest likelihood of successful migrants.
Less invasive replacement of aortic root, ascending aorta and hemiarch via partial upper sternotomy: a propensity-score-matched comparison with full sternotomy
Authors: Papadopoulos Nestoras , Ntinopoulos Vasileios , Haeussler Achim , Odavic Dragan , Risteski Petar , Biefer Héctor Rodríguez Cetina , Dzemali Omer 
Published: June 2024

Abstract

OBJECTIVES
Less invasive surgery has emerged as an option for aortic pathologies. The current study compared our experience on early postoperative results of patients with aortic surgery between partial upper sternotomy (PUS) and full sternotomy (FS).

METHODS
We performed a retrospective analysis of the data of patients undergoing aortic root surgery with concomitant ascending aorta and hemiarch replacement. Exclusion criteria were type A aortic dissection and other concomitant major cardiac surgery. After propensity score matching, we compared the perioperative outcomes of patients undergoing surgery with PUS versus FS.

RESULTS
A total of 161 patients operated on between January 2013 and September 2022 met the inclusion criteria (PUS: n = 22, FS: n = 139). Propensity score matching yielded 22 pairs with a balanced distribution of propensity scores and covariates between the compared groups. There was no evidence that PUS affects cardiopulmonary bypass [108 (67–119) vs 113 (87–148) min, P = 0.154; PUS vs FS] and circulatory arrest duration [9 (7–10) vs 9 (8–13) min, P = 0.264; PUS vs FS]. There was a reduced cross-clamp duration in the PUS group [88 (58–96) vs 92 (71–122) min, P = 0.032]. Cumulative sum charts have shown consistently low cross-clamp and circulatory arrest duration for 2 experienced surgeons who performed 20 of the procedures in the PUS group (10 each). Perioperative mortality and morbidity were low, with no in-hospital mortality in the PUS group [0 vs 1(4.5%), P &gt; 0.999] and absence of strokes in both groups.

CONCLUSIONS
In summary, our initial experience suggests that less invasive aortic root, ascending aorta and hemiarch replacement via PUS could be performed in our patient cohort as safely as via full sternotomy. Advantages for the patient are reduced surgical trauma, improved cosmetic results and—presumably—less pain.
Russia's Leadership in Eurasia: Holding Together or Falling Apart?
Authors: Roberts Sean , Ziemer Ulrike 
Published: June 2024

Abstracts
The Russo–Ukraine War raises important questions on the dynamics of regional leadership and followership in what may be termed “Russian-led Eurasia.” These questions, in particular, the strength of Russian leadership in the region is complicated by the ambiguity in existing literature and competing images of Russia's relations with long-standing allies—notably Armenia, Belarus, Kazakhstan and Kyrgyzstan—which are often portrayed in terms of a “community of fate” or partners destined for closer integration but also as a “community of fortune” or ad hoc, situational partners, loosely centered on Russia. This article offers an innovative theoretical and methodological exploration of Russia's relations with regional partner states by utilizing the English School of International Relations and regional integration organizations to assess Russia's regional leadership. As argued in this article, Russian-led Eurasia may be understood as an example of a regional interstate society with Russian hegemony serving as a socially conferred, binding institution. But this hegemony is inherently unstable owing to Russia's inability to balance hegemonic “rights” with “responsibilities.” War in Ukraine did not create this problem, but it has created the conditions for leadership transition in the region.