bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of Colorectal Carcinoma Stemness, Growth, and Metastasis by an miR-200c-Sox2–Negative Feedback Loop Mechanism
Authors: Lu Yan-Xia , Yuan Li , Xue Xiao-Lei , Zhou Min , Liu Yan , Zhang Chao , Li Jing-Ping , Zheng Lin , Hong Min , Li Xue-Nong 
Published: March 2014

Abstract
Purpose: To elucidate a novel mechanism of miR-200c in the regulation of stemness, growth, and metastasis in colorectal carcinoma (CRC).
Experimental Design: Quantitative reverse transcription PCR was used to quantify miR-200c expression in CRC cell lines and tissues. A luciferase assay was adopted for the target evaluation. The functional effects of miR-200c in CRC cells were assessed by its forced or inhibited expression using lentiviruses.
Results: MiR-200c was statistically lower in CRC clinical specimens and highly metastatic CRC cell lines compared with their counterparts. Sox2 was validated as a target for miR-200c. The knockdown of miR-200c significantly enhanced proliferation, migration, and invasion in CRC cell lines, whereas the upregulation of miR-200c exhibited an inverse effect. Moreover, rescue of Sox2 expression could abolish the effect of the upregulation of miR-200c. In addition, the reduction of miR-200c increased the expression of CRC stem cell markers and the sphere-forming capacity of CRC cell lines. Further study has shown that miR-200c and Sox2 reciprocally control their expression through a feedback loop. MiR-200c suppresses the expression of Sox2 to block the activity of the phosphoinositide 3-kinase (PI3K)–AKT pathway.
Conclusion: Our findings indicate that miR-200c regulates Sox2 expression through a feedback loop and is associated with CRC stemness, growth, and metastasis. Clin Cancer Res; 20(10); 2631–42. ©2014 AACR.
A Signature Predicting Poor Prognosis in Gastric and Ovarian Cancer Represents a Coordinated Macrophage and Stromal Response
Authors: Busuttil Rita A. , George Joshy , Tothill Richard W. , Ioculano Kylie , Kowalczyk Adam , Mitchell Catherine , Lade Stephen , Tan Patrick , Haviv Izhak , Boussioutas Alex 
Published: March 2014

Abstract
Purpose: Gene-expression profiling has revolutionized the way we think about cancer and confers the ability to observe the synchronous expression of thousands of genes. The use of putative genome-level expression profiles has allowed biologists to observe the complex interactions of genes that constitute recognized biologic pathways. We used gastric and ovarian datasets to identify gene-expression signatures and determine any functional significance.
Experimental Design: Microarray data of 94-tumor and 45-benign samples derived from patients with gastric cancer were interrogated using Hierarchical Ordered Partitioning and Collapsing Hybrid analysis identifying clusters of coexpressed genes. Clusters were further characterized with respect to biologic significance, gene ontology, and ability to discriminate between normal and tumor tissue. Tumor tissues were separated into epithelial and stromal compartments and immunohistochemical analysis performed to further elucidate specific cell lineages expressing genes contained in the signature.
Results: We identified a “stromal-response” expression signature, highly enriched for inflammatory, extracellular matrix, cytokine, and growth factor proteins. The majority of genes in the signature are expressed in the tumor-associated stroma but were absent in associated premalignant conditions. In gastric cancer, this module almost perfectly differentiates tumor from nonmalignant gastric tissue and hence can be regarded as a highly tumor-specific gene-expression signature.
Conclusions: We show that these genes are consistently coexpressed across a range of independent gastric datasets as well as other cancer types suggesting a conserved functional role in cancer. In addition, we show that this signature can be a surrogate marker for M2 macrophage activity and has significant prognostic implications in gastric and ovarian high-grade serous cancer. Clin Cancer Res; 20(10); 2761–72. ©2014 AACR.
Minimal Residual Disease in Breast Cancer: In Blood Veritas
Authors: Siravegna Giulia , Bardelli Alberto 
Published: March 2014

Abstract
A blood-based molecular test might direct recommendations for systemic therapies in patients with early-stage breast cancer undergoing surgery with curative intent. A new study suggests that droplet digital PCR (ddPCR) can be used to detect cancer-specific DNA alterations in plasma with sensitivity suitable for monitoring minimal residual disease. Clin Cancer Res; 20(10); 2505–7. ©2014 AACR.
Epithelial-to-Mesenchymal Transition Mediates Docetaxel Resistance and High Risk of Relapse in Prostate Cancer
Authors: Marín-Aguilera Mercedes , Codony-Servat Jordi , Reig Òscar , Lozano Juan José , Fernández Pedro Luis , Pereira María Verónica , Jiménez Natalia , Donovan Michael , Puig Pere , Mengual Lourdes , Bermudo Raquel , Font Albert , Gallardo Enrique , Ribal María José , Alcaraz Antonio , Gascón Pere , Mellado Begoña 
Published: March 2014

Abstract
Molecular characterization of radical prostatectomy specimens after systemic therapy may identify a gene expression profile for resistance to therapy. This study assessed tumor cells from patients with prostate cancer participating in a phase II neoadjuvant docetaxel and androgen deprivation trial to identify mediators of resistance. Transcriptional level of 93 genes from a docetaxel-resistant prostate cancer cell lines microarray study was analyzed by TaqMan low-density arrays in tumors from patients with high-risk localized prostate cancer (36 surgically treated, 28 with neoadjuvant docetaxel + androgen deprivation). Gene expression was compared between groups and correlated with clinical outcome. VIM, AR and RELA were validated by immunohistochemistry. CD44 and ZEB1 expression was tested by immunofluorescence in cells and tumor samples. Parental and docetaxel-resistant castration-resistant prostate cancer cell lines were tested for epithelial-to-mesenchymal transition (EMT) markers before and after docetaxel exposure. Reversion of EMT phenotype was investigated as a docetaxel resistance reversion strategy. Expression of 63 (67.7%) genes differed between groups (P &lt; 0.05), including genes related to androgen receptor, NF-κB transcription factor, and EMT. Increased expression of EMT markers correlated with radiologic relapse. Docetaxel-resistant cells had increased EMT and stem-like cell markers expression. ZEB1 siRNA transfection reverted docetaxel resistance and reduced CD44 expression in DU-145R and PC-3R. Before docetaxel exposure, a selected CD44+ subpopulation of PC-3 cells exhibited EMT phenotype and intrinsic docetaxel resistance; ZEB1/CD44+ subpopulations were found in tumor cell lines and primary tumors; this correlated with aggressive clinical behavior. This study identifies genes potentially related to chemotherapy resistance and supports evidence of the EMT role in docetaxel resistance and adverse clinical behavior in early prostate cancer. Mol Cancer Ther; 13(5); 1270–84. ©2014 AACR.
Contribution of p53 to Metastasis
Authors: Powell Emily , Piwnica-Worms David , Piwnica-Worms Helen 
Published: March 2014

Abstract
The tumor suppressor p53 is lost or mutated in about half of all human cancers, and in those tumors in which it is wild-type, mechanisms exist to prevent its activation. p53 loss not only prevents incipient tumor cells from undergoing oncogene-induced senescence and apoptosis, but also perturbs cell-cycle checkpoints. This enables p53-deficient tumor cells with DNA damage to continue cycling, creating a permissive environment for the acquisition of additional mutations. Theoretically, this could contribute to the evolution of a cancer genome that is conducive to metastasis. Importantly, p53 loss also results in the disruption of pathways that inhibit metastasis, and transcriptionally defective TP53 mutants are known to gain additional functions that promote metastasis. Here, we review the evidence supporting a role for p53 loss or mutation in tumor metastasis, with an emphasis on breast cancer.
Significance: The metastatic potential of tumor cells can be positively influenced by loss of p53 or expression of p53 gain-of-function mutants. Understanding the mechanisms by which p53 loss and mutation promote tumor metastasis is crucial to understanding the biology of tumor progression and how to appropriately apply targeted therapies. Cancer Discov; 4(4); 405–14. ©2014 AACR.
Near-Infrared II Fluorescence for Imaging Hindlimb Vessel Regeneration With Dynamic Tissue Perfusion Measurement
Authors: Hong Guosong , Lee Jerry C. , Jha Arshi , Diao Shuo , Nakayama Karina H. , Hou Luqia , Doyle Timothy C. , Robinson Joshua T. , Antaris Alexander L. , Dai Hongjie , Cooke John P. , Huang Ngan F. 
Published: March 2014

Background—
Real-time vascular imaging that provides both anatomic and hemodynamic information could greatly facilitate the diagnosis of vascular diseases and provide accurate assessment of therapeutic effects. Here, we have developed a novel fluorescence-based all-optical method, named near-infrared II (NIR-II) fluorescence imaging, to image murine hindlimb vasculature and blood flow in an experimental model of peripheral arterial disease, by exploiting fluorescence in the NIR-II region (1000–1400 nm) of photon wavelengths.

Methods and Results—

Because of the reduced photon scattering of NIR-II fluorescence compared with traditional NIR fluorescence imaging and thus much deeper penetration depth into the body, we demonstrated that the mouse hindlimb vasculature could be imaged with higher spatial resolution than in vivo microscopic computed tomography. Furthermore, imaging during 26 days revealed a significant increase in hindlimb microvascular density in response to experimentally induced ischemia within the first 8 days of the surgery (
P
<0.005), which was confirmed by histological analysis of microvascular density. Moreover, the tissue perfusion in the ischemic hindlimb could be quantitatively measured by the dynamic NIR-II method, revealing the temporal kinetics of blood flow recovery that resembled microbead-based blood flowmetry and laser Doppler blood spectroscopy.

Conclusions—
The penetration depth of millimeters, high spatial resolution, and fast acquisition rate of NIR-II imaging make it a useful imaging tool for murine models of vascular disease.
Counseling African Americans to Control Hypertension
Authors: Ogedegbe Gbenga , Tobin Jonathan N. , Fernandez Senaida , Cassells Andrea , Diaz-Gloster Marleny , Khalida Chamanara , Pickering Thomas , Schwartz Joseph E. 
Published: March 2014

Background—
Data are limited on the implementation of evidence-based multilevel interventions targeted at blood pressure (BP) control in hypertensive blacks who receive care in low-resource primary care practices.

Methods and Results—

Counseling African Americans to Control Hypertension is a cluster-randomized clinical trial in which 30 community health centers were randomly assigned to the intervention condition (IC) or usual care (UC). Patients at the IC sites received patient education, home BP monitoring, and monthly lifestyle counseling, whereas physicians attended monthly hypertension case rounds and received feedback on their patients’ home BP readings and chart audits. Patients and physicians at the UC sites received printed patient education material and hypertension treatment guidelines, respectively. The primary outcome was BP control, and secondary outcomes were mean changes in systolic and diastolic BPs at 12 months, assessed with an automated BP device. A total of 1059 patients (mean age, 56 years; 28% men, 59% obese, and 36% with diabetes mellitus) were enrolled. The BP control rate was similar in both groups (IC=49.3% versus UC=44.5%; odds ratio, 1.21 [95% confidence interval, 0.90–1.63];
P
=0.21). In prespecified subgroup analyses, the intervention was associated with greater BP control in patients without diabetes mellitus (IC=54.0% versus UC=44.7%; odds ratio, 1.45 [confidence interval, 1.02–2.06]); and small-sized community health centers (IC=51.1% versus UC=39.6%; odds ratio, 1.45 [confidence interval, 1.04–2.45]).

Conclusions—
A practice-based, multicomponent intervention was no better than UC in improving BP control among hypertensive blacks. Future research on the implementation of behavioral modification strategies for hypertension control in low-resource settings should focus on the development of more efficient and tailored interventions in this high-risk population.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT00233220.
Proteomic Analysis Implicates Translationally Controlled Tumor Protein as a Novel Mediator of Occlusive Vascular Remodeling in Pulmonary Arterial Hypertension
Authors: Lavoie Jessie R. , Ormiston Mark L. , Perez-Iratxeta Carol , Courtman David W. , Jiang Baohua , Ferrer Elisabet , Caruso Paola , Southwood Mark , Foster William S. , Morrell Nicholas W. , Stewart Duncan J. 
Published: March 2014

Background—

Pulmonary arterial hypertension (PAH) is a lethal disease characterized by excessive proliferation of pulmonary vascular endothelial cells (ECs). Hereditary PAH (HPAH) is often caused by mutations in the bone morphogenetic protein receptor type 2 gene (
BMPR2
). However, the mechanisms by which these mutations cause PAH remain unclear. Therefore, we screened for dysregulated proteins in blood-outgrowth ECs of HPAH patients with
BMPR2
mutations compared with healthy control subjects.

Methods and Results—
A total of 416 proteins were detected with 2-dimensional PAGE in combination with liquid chromatography/tandem mass spectrometry analysis, of which 22 exhibited significantly altered abundance in blood-outgrowth ECs from patients with HPAH. One of these proteins, translationally controlled tumor protein (TCTP), was selected for further study because of its well-established role in promoting tumor cell growth and survival. Immunostaining showed marked upregulation of TCTP in lungs from patients with HPAH and idiopathic PAH, associated with remodeled vessels of complex lesions. Increased TCTP expression was also evident in the SU5416 rat model of severe and irreversible PAH, associated with intimal lesions, colocalizing with proliferating ECs and the adventitia of remodeled vessels but not in the vascular media. Furthermore, silencing of TCTP expression increased apoptosis and abrogated the hyperproliferative phenotype of blood-outgrowth ECs from patients with HPAH, raising the possibility that TCTP may be a link in the emergence of apoptosis-resistant, hyperproliferative vascular cells after EC apoptosis.

Conclusion—
Proteomic screening identified TCTP as a novel mediator of endothelial prosurvival and growth signaling in PAH, possibly contributing to occlusive pulmonary vascular remodeling triggered by EC apoptosis.
Reshaping International Fisheries Development: Assimilating the Treaty on Fisheries between the Governments of Certain Pacific Island States and the United States under the PNA Vessel Day Scheme (VDS)
Authors: Aqorau Transform 
Published: March 2014

Abstract
Since June 1988, Pacific Island States and the United States have co-operated in fisheries management, conservation and development through the Treaty on Fisheries between the Governments of Certain Pacific Island States and the Government of the United States. The renegotiation of the Treaty has presented challenges. The Treaty worked perfectly under an open and unrestricted access regime. However, changes instituted by Pacific Island States and the Commission for the Conservation and Management of Highly Migratory Fish Stocks in the Western and Central Pacific have tested the utility of the Treaty. This article discusses these changes, and these challenges, including the implications of the Vessel Day Scheme. The article concludes that having unimpeded multiple-zone access is inimical to a rights-based system such as the purse-seine Vessel Day Scheme.
Voluntary Guidelines for Flag State Performance: A New Tool to Conquer IUU Fishing
Authors: Erikstein Karine , Swan Judith 
Published: March 2014

Abstract
Within the framework of the Food and Agriculture Organization of the United Nations (fao), Voluntary Guidelines for Flag State Performance were adopted by the fao Technical Consultation on Flag State Performance on 8 February 2013. The Guidelines, which focus on the role of the flag State in combating illegal, unreported and unregulated (iuu) fishing and build on existing international law, constitute a ground-breaking international instrument. They set out criteria and procedures for the assessment of flag State performance, as well as provisions on cooperation between flag States and coastal States, compliance and cooperation with, and assistance to, developing countries. The Guidelines were adopted as a response to the lack of effective control by many flag States over their fishing and fishing-related vessels, mindful that flag States may generally be said to have the primary responsibility for preventing, deterring and eliminating iuu fishing.1 The Guidelines are a robust and comprehensive tool for enhancing international fisheries governance.
Protection and Preservation of the Marine Environment as a Goal for Achieving Sustainable Development on the Rio+20 Agenda
Authors: Oanta Gabriela A. 
Published: March 2014

Abstract
The United Nations Convention on Sustainable Development, held in Rio de Janeiro between 20 and 22 June 2012, represented an excellent opportunity for learning the current state of the international community’s commitment to sustainable development. Undoubtedly, without sustainable seas and oceans it is impossible to talk about sustainability on our planet. Seas and oceans enjoy a privileged position within the global ecosystem as they represent 72% of the Earth’s surface area, accounting for 90% of the habitat of all life, and are a major source of economic, social and environmental resources. Hence the special interest in an effective protection of the global marine ecosystem, which is gravely threatened by a variety of factors, of which illegal, unreported and unregulated fishing is one. The present article will be divided into two parts. The first of these will study the goal of protecting the marine environment as part of the Rio+20 agenda, whilst the second will analyse illegal, unreported and unregulated fishing from a legal point of view, with particular attention being paid to the most significant challenges presented by this global scourge.
Depression and chronic kidney disease: A review for clinicians
Authors: Bautovich Alison , Katz Ivor , Smith Michelle , Loo Colleen K , Harvey Samuel B 
Published: March 2014

Objective: To review the recent academic literature surrounding the prevalence, aetiopathology, associations and management of depression in chronic kidney disease (CKD), in order to provide a practical and up-to-date resource for clinicians. Methods: We conducted electronic searches of the following databases: MEDLINE, EMBASE and PsycINFO. The main search terms were: depression, mood disorders, depressive disorder, mental illness, in combination with kidney disease, renal insufficiency, dialysis, kidney failure. Separate searches were conducted regarding antidepressant use in CKD. Results: A number of recent, large and well-conducted studies have confirmed markedly raised rates of depression amongst those with CKD, with meta-analysis suggesting the prevalence of interview-defined depression to be approximately 20%. The interactions between depression and CKD are complex, bidirectional and multifactorial. Depression in CKD has been shown to be associated with multiple poor outcomes, including increased mortality and hospitalisation rates, as well as poorer treatment compliance and quality of life. Clinical evaluation of depression in patients with CKD can be challenging; however, once a diagnosis is made, a range of treatment modalities can be considered. Conclusions: Depression is common in CKD and is associated with a significant risk of adverse outcomes. Given the importance of this issue, there is now an urgent need for well-conducted randomised trials of interventions for depression in CKD in order to provide information on the safety and efficacy of treatments.
Addressing failures in exascale computing
Authors: Snir Marc , Wisniewski Robert W , Abraham Jacob A , Adve Sarita V , Bagchi Saurabh , Balaji Pavan , Belak Jim , Bose Pradip , Cappello Franck , Carlson Bill , Chien Andrew A , Coteus Paul , DeBardeleben Nathan A , Diniz Pedro C , Engelmann Christian , Erez Mattan , Fazzari Saverio , Geist Al , Gupta Rinku , Johnson Fred , Krishnamoorthy Sriram , Leyffer Sven , Liberty Dean , Mitra Subhasish , Munson Todd , Schreiber Rob , Stearley Jon , Hensbergen Eric Van 
Published: March 2014

We present here a report produced by a workshop on ‘Addressing failures in exascale computing’ held in Park City, Utah, 4–11 August 2012. The charter of this workshop was to establish a common taxonomy about resilience across all the levels in a computing system, discuss existing knowledge on resilience across the various hardware and software layers of an exascale system, and build on those results, examining potential solutions from both a hardware and software perspective and focusing on a combined approach. The workshop brought together participants with expertise in applications, system software, and hardware; they came from industry, government, and academia, and their interests ranged from theory to implementation. The combination allowed broad and comprehensive discussions and led to this document, which summarizes and builds on those discussions.
Enseigne-t-on l’intervention en cessation tabagique aux futures infirmières ? Résultat d’une étude descriptive
Authors: Lepage Mario , Dumas Louise , Saint-Pierre Chantal 
Published: March 2014

Introduction : Les infirmières qui ont reçu une formation en cessation tabagique ont tendance à intervenir davantage auprès des fumeurs que celles qui n’ont pas été formées. Il est démontré qu’une intervention même très brève conduit à une diminution de la prévalence du tabagisme. Or, les programmes qui préparent à l’exercice de la profession infirmière laissent actuellement peu de place à la formation en cessation tabagique. Les objectifs de la recherche sont de décrire les pratiques d’enseignement de la cessation tabagique dans les programmes de formation infirmière et de comparer en tenant compte de la formation en cessation tabagique reçue par les professeures. Méthodologie : Un devis descriptif-comparatif a été choisi. Résultats : Au total, 278 professeures (20,8 %) ont répondu à un sondage en ligne effectué à l’aide d’un questionnaire validé. Celles-ci avouent offrir en moyenne une heure en cessation tabagique par année. Les aspects physiopathologiques sont couverts au détriment de l’intervention professionnelle en cessation tabagique. Les obstacles identifiés par les professeures sont le manque de temps, de connaissances et de pertinence avec le cours enseigné. Seulement 11,9 % des sujets mentionnent avoir reçu une formation spécifique en cessation tabagique. Or ce sont ces dernières qui enseignent davantage d’éléments reliés à l’intervention en cessation tabagique. Conclusion : Une meilleure intégration de l’intervention en promotion de la santé, incluant la cessation tabagique, dans la formation initiale devrait amener la future infirmière à réaliser davantage d’interventions personnalisées en cessation tabagique.