bluuebunny/crossref_metadata_2025_split huggingface dataset:
3‐D Analysis of Palatal Morphology Associated with Palatalized Articulation in Patients with Unilateral Cleft Lip and Palate
Authors: Nishikubo Mai , Hirahara Narihiro , Gomi Akinori , Nozoe Etsuro , Nakamura Norifumi 
Published: August 2012

AbstractPalatalized articulation is one of the major articulation disorders which patients with cleft palate face after palatoplasty. Various causal factors have been suggested to date, but the main cause remains poorly understood. To clarify the possible causes of palatalized articulation in speakers with cleft palate, three‐dimensional palatal morphology in patients with/without palatalized articulation in unilateral cleft lip and palate (UCLP) was analyzed.Twelve UCLP patients with palatalized articulation (P group) and 20 UCLP patients with normal articulation (N group) participated in the present study. Speech was assessed in the two groups at the age of about four. Dental casts of the maxilla taken at the same time were analyzed three‐dimensionally, and measurements in the horizontal, frontal, and sagittal planes were compared between patients with palatalized articulation and those with normal articulation. All dental casts were measured with a non‐contact 3D laser scanner and the 3D data were analyzed with 3D‐analyzing software.Our study demonstrated three major findings of palatal morphology in UCLP patients with palatalized articulation when compared with their non‐palatalized counterparts : 1) the posterior region of the palate was narrow in the horizontal plane, 2) asymmetry of the anterior palate was severe in the frontal plane, and 3) the palate was flat and shallow in the sagittal plane.These findings suggested that palate deformities can affect the lingual‐contact pattern, and may account for the backward movement of the articulation point.
Histological Investigation of the Development of the Anlage of the Mandibular Condyle of Mouse
Authors: Tsuchikawa Kohzo , Kumakura Masahiko , Kuwahara Toru , Ito Hidetoshi 
Published: August 2012

AbstractTo elucidate the histological findings of the anlage of the mandibular condyle during very early developmental stages, we analyzed sagittal and frontal plane serial sections of mouse fetuses for which the gestational period was precisely determined. An aggregate of mesenchymal cells around the buccal nerve (peripheral cell aggregate) could be seen at 12.0 days post‐conception (dpc). Another cell aggregate (core cell aggregate), which almost coincided with the outline of the condylar head, was detected on the inside of the dome‐shaped peripheral cell aggregate at 12.75 dpc. The cells of the peripheral cell aggregate were gradually flattened in accordance with cell differentiation, and formed a fibrous sheath covering the condylar head by 15.0 dpc. The cells of the central region of the core cell aggregate differentiated into hypertrophic chondrocytes by 14.5 dpc, whereas the cells of the fringe of the core cell aggregate differentiated into osteogenic cells to form the bone collar by 15.0 dpc. The continuity of the anlage of the condyle with that of the mandibular ramus was first recognized at 13.0 dpc. As the anlage of the mandibular condyle was observed histologically during very early developmental stages, further research is necessary to characterize the development of this anlage in greater detail.
Crosstalk between Blood Vessels and Tumor Microenvironment
Authors: Hida Kyoko , Ohga Noritaka , Kurosu Takuro , Totsuka Yasunori , Shindoh Masanobu 
Published: August 2012

AbstractTumor blood vessels play an important role in tumor progression and metastasis. Thus, targeting tumor blood vessels is an important strategy for cancer therapy, especially for head and neck cancer patients. Tumor blood vessels generally sprout from pre‐existing vessels and have been thought to be genetically normal. However, tumor blood vessels have been shown to differ from their normal counterparts, for example, by changes in morphology. The authors isolated tumor endothelial cells (TECs) from mouse tumor xenografts and have shown that the TECs are abnormal. TECs up‐regulate many genes and proliferate more rapidly and migrate more than normal endothelial cells (NECs). Furthermore, TECs were found to be cytogenetically abnormal. We conclude that TECs can acquire cytogenetic abnormalities while in a tumor microenvironment.To develop ideal antiangiogenic therapies, understanding the crosstalk between blood vessels and the tumor microenvironment is important.Here, we provide an overview of the current studies on TEC abnormalities and a discussion about possible mechanisms for how tumor the microenvironment makes TECs abnormal.
Aberrant Control of Transcription and Transportation of Oncogenic mRNA in Carcinogenesis
Authors: Higashino Fumihiro , Hida Kyoko , Kitamura Tetsuya , Ohga Noritaka , Shindoh Masanobu 
Published: August 2012

AbstractMultiple gene disorders have been shown to be involved in carcinogenesis. Mutation, translocation and amplification have been identified in so‐called oncogenes, and inactivation of antioncogenes by mutation and deletion has been shown. E1AF is an etsoncogene family transcription factor, and has been shown to upregulate multiple matrix metalloproteinase (MMP) genes that contribute to the malignant phenotype of cancer cells by inducing invasive and metastatic activities. EWS/ETS fusions are frequently observed in Ewing’s sarcoma, and we have revealed that EWS/ETS chimeric protein activates telomerase activity by upregulating human telomerase reverse transcriptase (hTERT), but the transcriptional activation of hTERT by EWS/ETS was indirect, and EWS/ETS was seen to function as a co‐activator for TERT transcription. A number of oncogenes and cancerrelated genes contain AU‐rich element (ARE) in non‐coding regions of transcribed mRNA. HuR is a RNA‐binding protein that has the potential to stabilize ARE‐containing mRNAs. HuR is known to shuttle between the nucleus and the cytoplasm via several export pathways. When normal cells are exposed to stress, HuR is exported to the cytoplasm in a chromosome maintenance region 1 (CRM1)‐dependent manner. However, we demonstrate that HuR is CRM‐1 independently exported to the cytoplasm in oral cancer cells. ARE‐mRNAs were also exported to the cytoplasm and stabilized in the oral cancer cells, which were inhibited by HuR knockdown. These findings suggest that transcriptional and translational abnormalities of oncogenes may contribute to the carcinogenesis of oral epithelial cells.
Tissue Reaction to Synthetic Oligopeptide Derived from Enamel Matrix Derivative in Rats
Authors: Hida Tomoaki , Tominaga Kazuya , Tanaka Akio 
Published: August 2012

AbstractBackground: Enamel matrix derivative placed in the backs of rats induces numerous eosinophilic round bodies (ERBs) and hard tissue. ERBs were analyzed by matrix assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) and by database analysis. Subsequently, a seven‐amino‐acid sequence was identified, and an artificial oligopeptide was synthesized with its sequence. The present experiment was carried out to clarify whether or not the peptide induces hard tissue formation in vivo.Methods: Synthetic oligopeptide was injected into the backs of 15 rats. After injection, the tissues were excised at various times and prepared for light and immune‐light microscopy.Results: A white lump was macroscopically observed in each rat back 14 days after injection of 7.5 and 15 mg/mL synthetic oligopeptide, and endochondral ossification and bone formation were microscopically observed in one rat back 14 days after injection of 15 mg/mL synthetic oligopeptide.Conclusions: The synthetic oligopeptide is pure, 1,118 dalton, and apparently low‐toxicity. It seems to produce hard tissues, such as cartilage and bone tissue.
Anaphylactic Reaction Induced by Diclofenac: A Case Report
Authors: Sanuki Takuro , Sugioka Shingo , Kotani Junichiro 
Published: August 2012

AbstractAn anaphylactic reaction is a most severe immune‐mediated reaction. Diclofenac is a widely used non‐steroidal anti‐inflammatory drug (NSAID) derivative of phenylacetic acid, and continues to be routinely prescribed. We report a case of an anaphylactic reaction induced by oral diclofenac.
Concealed WPW Syndrome with Longitudinal Dissociation in the His Bundle Exhibiting Five Different Electrocardiographic Waveforms During Tachycardia
Authors: Kato Isao , Iwa Toru , Suzuki Yasushi , Ito Takayuki 
Published: August 2012

We examined a patient with concealed WPW syndrome who exhibited five different electrocardiographic waveforms during tachycardia.With the intracardiac electrogram, all tachycardia were artrioventricular reciprocating tachycardia with retrograde conduction through only the accessory pathway where each artrioventricular conduction form varied: i.e., one with anterograde conduction through the fast pathway, one with anterograde conduction through the slow pathway, one with anterograde conduction through the fast pathway and slow pathway alternately, and an irregular R‐R interval, one with anterograde conduction through the fast pathway and wide QRS tachycardia with the right bundle branch block type, and one with anterograde conduction through the fast pathway and wide QRS tachycardia with left bundle branch block.His bundle electrogram might be split and H, H′ was recorded at the time of sinus rhythm. Furthermore, the right and left bundle branches exhibited a different refractory period because of longitudinal dissociation in the His bundle. Therefore wide QRS tachycardia with both right and left bundle branch block might appear without complete atrioventricular block.In addition to the association between fast pathway and slow pathway, right and left bundle branch block patterns appeared.
Efficacy of Cardiac Resynchronization Therapy Performed Concomitantly with Primary Cardiac Surgery
Authors: Morimoto Keisuke , Ando Kenji , Kitaoka Hiroaki , Yamada Takayuki , Nishiyama Kei , Sakai Kouyu , Nosaka Hideyuki , Doi Yoshinori , Nobuyoshi Masakiyo 
Published: August 2012

Cardiac resynchronization therapy (CRT) improves symptoms and cardiac function in patients with heart failure and prolongs QRS duration on an electrocardiogram. However, additional effects of concomitant CRT in patients who undergo cardiac surgery are unclear. We assessed the clinical efficacy of this therapy when it is performed concomitantly with primary cardiac surgery.METHODS: We evaluated four patients who underwent primary surgery and CRT between February 2002 and December 2003, and compare clinical data with four patients who underwent conventional primary surgery without CRT.RESULTS: Primary surgeries were CABG (n = 2), aortic valve replacement (n = 1), mitral valve plasty (n = 1) and mitral annular plication (n = 4). After the operation, symptoms associated with congestive heart failure were improved in all patients. The average NYHA class improvement was from 3.3 ± 0.5 before the operation to 1.7 ± 0.6 after the operation. All patients were discharged after surgery. Only one patient needed re‐hospitalization for congestive heart failure one month after the operation. The mean QRS duration (190 ± 47.6 ms vs 160 ± 16.3 ms), cardiothoracic ratio (60.7 ± 5.7% vs 58.5 ± 2.9%) and ejection fraction (28.5 ± 8.8% vs 50.0 ± 0.0%) were also improved after surgery.CONCLUSION: In patients with poor left ventricular function and impaired cardiac conduction, CRT performed concomitantly with primary surgery may improve the postoperative course. Further study on indications for implantation of a CRT device during primary surgery is needed.
Electrical Remodeling in Persistent Atrial Fibrillation May Be Mediated by Changes in the IKATP Channel
Authors: Asano Taku , Kobayashi Youichi , Miyata Akira , Miyoshi Fumito , Matsuyama Takaaki , Watanabe Norikazu , Minoura Yoshino , Liu Chungchang , Kawamura Mitsuharu , Tanno Kaoru , Katagiri Takashi 
Published: August 2012

The goal of this study was to measure the effective refractory period (ERP), the conduction velocity (CV) and the wavelength (WL) after cardioversion in patients with persistent atria] fibrillation (AF) and to determine the effects of the adenosine triphosphate sensitive potassium channel (KATP) opening agent, nicorandil, on those parameters in patients with persistent AF.METHODS: Patients with AF underwent elective cardioversion followed by measurement of ERP and CV before and after administration of nicorandil. Parameters were measured again one week later, and the ERP and the CV was used to calculate WL.RESULTS: ERP was significantly shorter immediately after termination of AF than at the 1‐week time point (193.4 vs. 228.7 msec p < 0.01). While there was no significant difference in ERP immediately after termination of AF when comparing measurements taken before and after the administration of nicorandil, ERP at the 1‐week time point was shorter after nicorandil administration than before nicorandil administration (193.4 vs. 191.4 msec, n.s.; 228.7 vs. 217.2msec, p < 0.01). Further, WL was higher at the 1‐week time point after nicorandil administration than before nicorandil administration.CONCLUSIONS: These data indicate that the electrical remodeling that occurs after cardioversion is at least partially mediated by changes in KATP channel behavior. Further, the electrophysiologic properties, that is, nicorandil prolonging the WL, may be of benefit in reducing the recurrence rate of AF.
Outcome of Total Pulmonary Vein Isolation in Patients with Persistent Atrial Fibrillation
Authors: Matsuo Seiichiro , Yamane Teiichi , Inada Keiichi , Shibayama Kenri , Miyanaga Satoru , Date Taro , Miyazaki Hidekazu , Sugimoto Kenichi , Mochizuki Seibu 
Published: August 2012

Background: Although the efficacy of pulmonary vein (PV) isolation for paroxysmal atrial fibrillation (AF) has been well‐established, its effect on persistent AF has not been sufficiently established to date.Methods and Results: In 68 consecutive patients (mean age, 52 ± 10 years) with paroxysmal (45) and persistent (23) AF, isolation of all four PVs was performed and the subsequent clinical outcome was evaluated. In total, 268/272 PVs (99%) were completely isolated from the left atrium by radiofrequency applications. During a mean follow‐up period of 11 months, 84% of patients with paroxysmal AF and 57% of patients with persistent AF were free from symptomatic AF without any antiarrhythmic drug (AAD) therapy (p = 0.04). In the remaining recurrent AF patients, no significant difference between the paroxysmal and persistent AF was observed as long as they took AADs which had been ineffective at baseline (freedom from AF; 98% and 96%, respectively, p = NS). Repeat procedure performed in the 12 recurrent patients (paroxysmal AF 6, persistent AF 6) allowed 11 (92%) of them to become free from AF recurrence without AADs.Conclusion: Electrical isolation of PV by standard catheter technique is equally and highly effective for both paroxysmal and persistent AF patients, when all four PVs were isolated.
Sluggish Upstroke of Signal‐Averaged QRS Complex. An Arrhythmogenic Sign in Patients with Anteroseptal Myocardial Infarction
Authors: Kanemura Masafumi , Katoh Takao , Tanaka Takashi , Kamei Shin‐ichiro , Kuroki Shin‐ichi , Takano Teruo , Hayakawa Hirokazu 
Published: August 2012

Non‐invasive recording of the late potential (LP) by signal‐averaging technique is useful in predicting ventricular tachycardia (VT), especially in patients with myocardial infarction (MI). However, the rate of positive LP is much lower in anterior or anteroseptal MI than inferior MI since the LP is sometimes masked by QRS complex. The purpose of the present study is firstly, to detect local conduction delay in initial portion of QRS complex and secondarily, to evaluate the clinical significance of the initial delay in patients with MI. Eighty patients with MI were analyzed in 4 groups as follows: AS: anteroseptal, n = 18, including 6 VT; A: anterior, n = 22, 7 VT; I: inferior, n = 28, 9 VT; and P: posterior, n = 12, noVT. Twenty non‐MI patients were used as normal controls (N). A signal processor (NEC 7T 18) was used to record signal‐averaged electrocardiograms and each 10 msec integral value of filtered QRS complex from the initial upstroke to the 60 msec point was automatically measured.The integral values of groups AS and A were lower than those of groups I, P, and N. AS patients with VT showed a lower integral value than AS patients without VT, but no differences were observed between A patients with VT and A patients without VT.We conclude that there is a much more sluggish upstroke in the signal‐averaged initial QRS complex in anteroseptal MI patients with VT, suggesting that the local conduction delay at the ventricular septum plays an important role in the mechanism of VT in these patients.
Idiopathic Premature Ventricular Contraction Originating from Left Epicardial Outflow Tract —Effects of Antiarrhythmic Drugs, Autonomic Provocation and Radiofrequency Catheter Ablation—
Authors: Miyaji Kotaro , Nishizaki Mitsuhiro , Ogawa Toru , Sugawara Shinji , Fujii Hiroyuki , Ashikaga Takashi , Yamawake Noriyoshi , Sakurada Harumizu , Hiraoka Masayasu 
Published: August 2012

AbstractWe present a patient with frequent episodes of palpitation who had idiopathic premature ventricular contraction (PVC. with QRS morphology exhibiting a right bundle branch block pattern with an inferior axis. Neither administration of procainamide, propranolol, lidocaine or verapamil could inhibit PVC, whereas administration of adenosine triphosphate, carotid sinus massage and Valsalva maneuver could effectively eliminate PVC. The earliest activation preceding the QRS complex by 25 msec at occurrence of the PVC was recorded at the distal site of the coronary sinus and within the left coronary cusp. Pacemapping in the supravalvular site during sinus rhythm produced a QRS complex more similar to PVC than that produced in the infravalvular site of the left ventricular outflow tract (LVOT). Radiofrequency catheter ablation was performed at the supravalvular site close to the epicardial LVOT, which resulted in successful elimination of PVCs.
Coronary Sinus Morphology in Patients with Posteroseptal Atrioventricular Accessory Pathways
Authors: Takenaka Sou , Nakano Yukiko , Hirao Hidekazu , Teragawa Hiroki , Shingu Tetsuji , Chayama Kazuaki 
Published: August 2012

AbstractBackground: There have been numerous reports about coronary sinus (CS) anomalies related to posteroseptal accessory pathways (APs). The purpose of this study was to explore the diameter and morphology of CS in patients with posteroseptal APs.Methods: We performed direct CS angiography in 105 patients with 22 posteroseptal APs and 83 APs in other regions, and 25 control subjects. We compared the diameter of the CS ostium in all subjects, and assessed the correlation of the local activation time in the patients with posteroseptal APs.Results: The proximal size (diameter) of the CS in the patients with posteroseptal APs (13:6 ± 1:1 mm) was larger than that in the patients with other types of APs (10:2 ± 1:8 mm [p < 0:001]) and that in the control subjects (9:6 ± 1:5 mm [p < 0:001]). Dilatation of the CS in the patients with posteroseptal APs extended up to 20 mm inside the CS. In 15 (68%) of the patients with posteroseptal APs, the proximal site of the CS demonstrated a windsock appearance.Conclusions: We concluded that the larger size and the wind cone appearance of proximal CS were unique structural characteristics in most patients with posteroseptal APs.
Effects of Music Therapy on Heart Rate Variability in Elderly Patients with Cerebral Vascular Disease and Dementia
Authors: Kurita Akira , Takase Bonpei , Okada Kaoru , Horiguchi Yuji , Abe Shinya , Kusama Yoshiki , Atarasi Hirotsugu 
Published: August 2012

AbstractObjective: In Japan, the number of elderly people with cerebral vascular disease (CVD) and dementia is increasing, resulting in increased expenditures to treat such patients. Reports indicate that music therapy can reduce anxiety in patients with myocardial infarction. However, it is unclear whether music therapy can have beneficial effects on very elderly patients with CVD and dementia, as these conditions may influence cardiac autonomic nerve functions. Therefore, we investigated the effects of music therapy on the autonomic nerve system in elderly patients with CVD and dementia, using an ambulatory Holter ECG system.Methods and Results: The subjects were 12 elderly patients aged ≥75 years, hospitalized for CVD and dementia. We measured their heart rate variability (HRV) indices of power domain and frequency domain analysis. The mean RR significantly increased during music therapy, from 842 ± 174 to 1022 ± 284 (p < 0:05), and decreased to 820 ± 284 ms after music therapy. HF significantly increased during music therapy, from 59 ± 41 to 127 ± 97 ms2 (p < 0:01), and decreased to 77 ± 60 ms2 after music therapy. LF/HF decreased from 2:2 ± 1:3 to 1:7 ± 1:3 during music therapy (p < 0:10), and increased to 2:0 ± 1:1 after music therapy. Time domain (HRV) indices increased during music therapy and decreased after music therapy: RMSSD increased from 19:4 ± 11 to 39:8 ± 17 ms, and then decreased to 19:4 ± 8:3 ms; pNN50 increased from 3:6 ± 3:2 to 16:1 ± 13:3%(p < 0:01), and then decreased to 4:3 ± 4:2%.Conclusions: Music therapy enhanced parasympathetic activity and decreased sympathetic activity in elderly patients with CVD and dementia. These findings suggest that music therapy is useful for alleviating anxiety, increasing comfort and facilitating relaxation for elderly patients with CVD and dementia.
The Rho/Rho‐kinase Systems Are Involved in Rapid Pacing‐induced Changes of Atrial Refractory Period in a Canine Model
Authors: Furusho Hiroshi , Sakagami Satoru , Takamura Masayuki , Kitano Katsunori , Abe Tsuyoshi , Hirazawa Motoaki , Usui Soichiro , Okajima Masaki , Saeki Takahiro , Maruyama Michiro , Kaneko Shuichi , Takata Sigeo 
Published: August 2012

AbstractIntroduction: The Rho/Rho‐kinase pathway has been related to various physiological responses of the cardiovascular system. Previous reports have suggested a significant effect of Rho signals on the electrophysiological characteristics of the heart. We hypothesized that the Rho/Rho‐kinase system would contribute to the rapid pacing‐related change of atrial effective refractory period (AERP).Methods and Results: In 17 dogs, AERP was measured at the right atrial appendage (RAA) and posterior left atrium (LA) before, during, and after 6‐hours rapid atrial pacing at 500 bpm. Saline control (n = 5), verapamil (n = 5), or fasudil (n = 7) were infused throughout the protocol. The shortening of AERP after rapid pacing was abrogated by the administration of verapamil, as reported in previous studies. Furthermore, fasudil (Rho/Rho‐kinase inhibitor) influenced the change of AERP in a manner similar to the infusion of verapamil throughout the experiments.Conclusions: Since the AERP was attenuated by fasudil, rapid pacing‐related atrial electrophysiological changes might involve the Rho/Rho‐kinase pathway.
Use of Catheter Ablation in the Treatment of Ventricular Tachycardia Triggered by Premature Ventricular Contraction
Authors: Kato Isao , Iwa Toru , Suzuki Yasushi , Ito Takayuki 
Published: August 2012

AbstractA 50‐year‐old man who had suffered from old myocardial infarction presented with an episode of syncope. DC shock was required for the interruption of frequent pleomorphic ventricular tachycardia (VT). Although the treatment for heart failure decreased the frequency of VT attacks, hemodynamically unstable VT occurred several times. A 12‐lead Holter electrocardiogram was used to determine the triggering premature ventricular contraction (PVC) and catheter ablation was performed by targeting this PVC. The site of origin of the triggering PVC was considered to be located between damaged cardiac muscle and intact Purkinje's fiber. No episode of PVC and VT was observed after a few days of ablation. An implantable cardioverter defibrillator was implanted but VT did not recur for more than 20 months.