bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a spray freeze‐drying method for preparation of volatile shiitake mushroom (Lentinus edodes) powder
Authors: Her Jae‐Young , Kim Min Suk , Kim Mina K. , Lee Kwang‐Geun 
Published: July 2015

SummaryIn this study, a method to produce a fine volatile powder extracted from shiitake mushrooms using spray freeze‐drying (SFD) was investigated. The analysis of the water‐soluble aromatic compounds was carried out by headspace solid‐phase micro‐extraction coupled with gas chromatography–mass spectrometry. Scanning electron microscopy and laser particle size analysis were applied to characterise the physical structure and size distribution of the SFD‐derived particles. Eleven key volatile compounds were identified in the extracts of shiitake mushrooms pre‐ and post‐SFD. Recoveries of aromatic volatiles ranging from 30.9% to 82.9% were observed in the overall flavour profile results from the powder obtained with SFD. Scanning electron microscopy analysis demonstrated that the particles of the aromatic powder were spherical in nature, having highly porous surfaces and mean diameters of 19.3 μm.
The Quality of Spontaneous Movements of Preterm Infants: Associations with the Quality of Mother–Infant Interaction
Authors: Lev‐Enacab Orna , Sher‐Censor Efrat , Einspieler Christa , Daube‐Fishman Galia , Beni‐Shrem Sara 
Published: July 2015

The assessment of the quality of infants’ spontaneous movements is a diagnostic tool for the young nervous system. We examined whether it relates to the quality of the interactions between infants born preterm and their mothers. Thirty‐nine healthy infants born preterm (Mage in weeks = 14.59, SD = 2.21; 38.46% female) and their mothers participated in the study. Infants’ quality of spontaneous movements was assessed using the General Movement Assessment according to Prechtl (Prechtl, Early Human Development 1990, 23, 151). A new measurement was employed to evaluate the following aspects of the mother–infant interaction: maternal sensitivity in the motor modality (i.e., when touching, picking up, holding, and putting down the infant), maternal sensitivity in the modalities of vocalization and eye contact, and infants’ positive engagement. Several aspects of infants’ quality of movements were associated with maternal sensitivity in the motor modality and infants’ positive engagement, but not with maternal sensitivity in the modalities of vocalization and eye contact. These findings suggest that the quality of infants’ spontaneous movements may explain some of the variability in the interactions between infants born preterm and their mothers. The results also highlight the importance of differentiating between the modalities of mothers’ behavior when assessing their sensitivity. Implications for research and practice with families of preterm infants are discussed.
Fungal Endophytes Enhance Agronomically Important Traits in Severely Drought‐Stressed Barley
Authors: Murphy B. R. , Martin Nieto L. , Doohan F. M. , Hodkinson T. R. 
Published: July 2015

AbstractCrops often face severe and damaging local drought events, and in some regions, these episodes are predicted to become more frequent due to climate change. Some micro‐organisms have been shown to improve drought tolerance and improve yield in crop plants. Here, we show that fungal root endophytes isolated from a wild barley species (Hordeum murinum subsp. murinum) induced significant improvements in agronomic traits for a severely drought‐stressed barley cultivar grown in a controlled environment, including number of tillers, grain yield and shoot biomass. Five endophyte strains were tested, and the trait that showed the greatest significant difference in the drought‐stressed plants was the number of tillers, where all of the endophyte treatments induced a greater number of tillers per plant. However, except in one case, the mean dry root weight for all plants was greater in the control plants, indicating preferential allocation of resources to aboveground parts in the endophyte treatments. Results were not consistent across all endophyte treatments, with some endophytes performing much better than others. As these growth studies were conducted using soil‐based compost, the results may translate to the field and suggest that some of these endophytes have potential as barley inoculants in arid growing conditions.
Safety and Clinical Outcome of Catheter Ablation of Ventricular Arrhythmias Using Contact Force Sensing: Consecutive Case Series
Authors: HENDRIKS ASTRID ARMANDA , AKCA FERDI , DABIRI ABKENARI LARA , KHAN MUCHTIAR , BHAGWANDIEN ROHIT , YAP SING‐CHIEN , WIJCHERS SIP , SZILI‐TOROK TAMAS 
Published: July 2015

Contact Force in Ventricular Tachycardia AblationBackgroundPoor catheter‐to‐myocardial contact can lead to ineffective ablation lesions and suboptimal outcome. Contact force (CF) sensing catheters in ventricular tachyarrhythmia (VT) ablations have not been studied for their long‐term efficacy.PurposeThe aim of this study was to compare CF ablation to manual ablation (MAN) and remote magnetic navigation (RMN) ablation for safety and efficacy in acute and long‐term outcome.MethodsA total of 239 consecutive patients who underwent VT ablation with the use of MAN, CF, or RMN catheters were included in this single‐center cohort study from January 2007 until March 2014. The primary endpoints were procedural success, acute major complications, and VT recurrences at follow‐up. The median follow‐up period was 25 months.ResultsAcute success was achieved in 182 out of 239 procedures (76%). Acute success in manual ablation, CF ablation and RMN ablation was 71%, 71%, and 86%, respectively (P = 0.03). Major complications occurred in 3.3% and there were less major complications (P = 0.04) in the RMN group. After an initial successful procedure, 66 of 182 patients (36%) had a recurrence during follow‐up. This was not significantly different between groups. Using an intention‐to‐treat analysis, 124 patients (52%) had a recurrence. The recurrence rate was lowest in the RMN group.ConclusionThe use of CF sensing catheters did not improve procedural outcome or safety profile in comparison to non‐CF sensing ablation in this observational study of ventricular arrhythmia ablations.
Determination of Shelf Life for Sausages Produced From Some Freshwater Fish Using Two Different Smoking Methods
Authors: Özpolat Emine , Patir Bahri 
Published: July 2015

AbstractIn the present study, microbiological, chemical and sensory changes of sausage samples produced from fillets of Chondrostoma regium, Luciobarbus mystaceus and Capoeta trutta implemented with traditional and liquid smoke were investigated during a storage period of 70 days at 4 ± 1C. The results of microbiological, physicochemical, chemical and sensory analyses showed that there were no differences between sausages smoked with either liquid smoke or traditional smoke (P > 0.05). As a result, each of the three fish species can be used in the production of sausage; there was no significant difference in terms of implementation of traditional or liquid smoke in product quality, but liquid smoke is more practical for implementation. The quality of the vacuum‐packed and stored sausage samples decreased during storage, but the sausages were of satisfactory eating quality until approximately 56 days of storage.Practical ApplicationsWith this study, we determined that fish species without economic importance could be used for industrial targets by using those species as a meat resource for sausage production. We also found that liquid smoke was easy to use for sausage production and it did not have any negative effects in terms of sensory, microbiological and chemical quality of fish sausage.
Electroporation Enhances Bleomycin Efficacy in Cats with Periocular Carcinoma and Advanced Squamous Cell Carcinoma of the Head
Authors: Spugnini E.P. , Pizzuto M. , Filipponi M. , Romani L. , Vincenzi B. , Menicagli F. , Lanza A. , De Girolamo R. , Lomonaco R. , Fanciulli M. , Spriano G. , Baldi A. 
Published: July 2015

BackgroundAdvanced carcinoma of the head represents a substantial health problem in cats for local control and overall survival.ObjectivesEvaluate the capability of electrochemotherapy (ECT) to improve bleomycin efficacy in cats with periocular carcinoma and advanced carcinoma of the head.AnimalsTwenty‐one cats with periocular carcinoma (17 squamous cell carcinoma [SCC] and 4 anaplastic carcinoma) and 26 cats with advanced SCC of the head.MethodsNonrandomized prospective controlled study. Periocular carcinoma cohorts: 12 cats were treated with bleomycin (15 mg/m2 IV) coupled with ECT under anesthesia; 9 cats were treated with bleomycin alone. Advanced head SCC cohorts: 14 cats were treated with bleomycin (15 mg/m2 IV) coupled with ECT administered under sedation; 12 control cats were treated with bleomycin alone. ECT treatments (2–8) were performed every other week until complete remission (CR) or tumor progression occurred.ResultsToxicities were minimal and mostly treated symptomatically. Overall response rate in the ECT treated animals was 89% (21 Complete Response [CR] and 2 Partial Response [PR]) whereas controls had response rate of 33% (4 CR and 3 PR). Median time to progression in ECT group was 30.5 months, whereas in controls it was 3.9 months (P < .0001). Median time to progression for ECT cohorts was 24.2 months for periocular cohort and 20.6 in advanced head SCC cohort, respectively.ConclusionsElectrochemotherapy is well tolerated for advanced SCC of the head in cats; its use may be considered among loco‐regional strategies for cancer therapy in sensitive body regions such as periocular region.
Peripheral phenotype and gene expression profiles of combined liver–kidney transplant patients
Authors: Dumontet Erwan , Danger Richard , Vagefi Parsia A. , Londoño Maria‐Carlota , Pallier Annaïck , Lozano Juan José , Giral Magali , Degauque Nicolas , Soulillou Jean‐Paul , Martínez‐Llordella Marc , Lee Herman , Latournerie Marianne , Boudjema Karim , Dulong Joelle , Tarte Karin , Sanchez‐Fueyo Alberto , Feng Sandy , Brouard Sophie , Conchon Sophie 
Published: July 2015

AbstractBackground and AimsThe beneficial effect of one graft on another has been reported in combined transplantation but the associated mechanisms and biological influence of each graft have not yet been established.MethodsIn multiple analyses, we explored the PBMC phenotype and signature of 45 immune‐related messenger RNAs and 754 microRNAs from a total of 235 patients, including combined liver–kidney transplant recipients (CLK), patients with a liver (L‐STA) or kidney (K‐STA) graft only under classical immunosuppression and patients with tolerated liver (L‐TOL) or kidney grafts (K‐TOL).ResultsCLK show an intermediary phenotype with a higher percentage of peripheral CD19+CD24+CD38Low memory B cells and Helios+ Treg cells, two features associated with tolerance profiles, compared to L‐STA and K‐STA (P < 0.05, P < 0.01). Very few miRNA were significantly differentially expressed in CLK vs. K‐STA and even fewer when compared to L‐STA (35 and 8, P < 0.05). Finally, CLK are predicted to share common miRNA targets with K‐TOL and even more with L‐TOL (344 and 411, P = 0.005). Altogether CLK display an intermediary phenotype and gene profile, which is closer to that of liver transplant patients, with possible similarities with the profiles of tolerant patients.ConclusionThese data suggest that CLK patients show the immunological influence of both allografts with liver having a greater influence.
Protein kinase C is essential for viability of the rice blast fungus Magnaporthe oryzae
Authors: Penn Tina J. , Wood Mark E. , Soanes Darren M. , Csukai Michael , Corran Andrew John , Talbot Nicholas J. 
Published: July 2015

SummaryProtein kinase C constitutes a family of serine–threonine kinases found in all eukaryotes and implicated in a wide range of cellular functions, including regulation of cell growth, cellular differentiation and immunity. Here, we present three independent lines of evidence which indicate that protein kinase C is essential for viability of Magnaporthe oryzae. First, all attempts to generate a target deletion of PKC1, the single copy protein kinase C‐encoding gene, proved unsuccessful. Secondly, conditional gene silencing of PKC1 by RNA interference led to severely reduced growth of the fungus, which was reversed by targeted deletion of the Dicer2‐encoding gene, MDL2. Finally, selective kinase inhibition of protein kinase C by targeted allelic replacement with an analogue‐sensitive PKC1AS allele led to specific loss of fungal viability in the presence of the PP1 inhibitor. Global transcriptional profiling following selective PKC inhibition identified significant changes in gene expression associated with cell wall re‐modelling, autophagy, signal transduction and secondary metabolism. When considered together, these results suggest protein kinase C is essential for growth and development of M. oryzae with extensive downstream targets in addition to the cell integrity pathway. Targeting protein kinase C signalling may therefore prove an effective means of controlling rice blast disease.
The Plant Genome Integrative Explorer Resource: PlantGenIE.org
Authors: Sundell David , Mannapperuma Chanaka , Netotea Sergiu , Delhomme Nicolas , Lin Yao‐Cheng , Sjödin Andreas , Van de Peer Yves , Jansson Stefan , Hvidsten Torgeir R. , Street Nathaniel R. 
Published: July 2015

Summary

Accessing and exploring large‐scale genomics data sets remains a significant challenge to researchers without specialist bioinformatics training.

We present the integrated PlantGenIE.org platform for exploration of Populus, conifer and Arabidopsis genomics data, which includes expression networks and associated visualization tools. Standard features of a model organism database are provided, including genome browsers, gene list annotation, Blast homology searches and gene information pages. Community annotation updating is supported via integration of WebApollo.

We have produced an RNA‐sequencing (RNA‐Seq) expression atlas for Populus tremula and have integrated these data within the expression tools. An updated version of the ComPlEx resource for performing comparative plant expression analyses of gene coexpression network conservation between species has also been integrated.

The PlantGenIE.org platform provides intuitive access to large‐scale and genome‐wide genomics data from model forest tree species, facilitating both community contributions to annotation improvement and tools supporting use of the included data resources to inform biological insight.
Arctic plant origins and early formation of circumarctic distributions: a case study of the mountain sorrel, Oxyria digyna
Authors: Wang Qian , Liu Jianquan , Allen Geraldine A. , Ma Yazhen , Yue Wei , Marr Kendrick L. , Abbott Richard J. 
Published: July 2015

Summary

Many plant species comprising the present‐day Arctic flora are thought to have originated in the high mountains of North America and Eurasia, migrated northwards as global temperatures fell during the late Tertiary period, and thereafter attained a circumarctic distribution. However, supporting evidence for this hypothesis that provides a temporal framework for the origin, spread and initial attainment of a circumarctic distribution by an arctic plant is currently lacking.

Here we examined the origin and initial formation of a circumarctic distribution of the arctic mountain sorrel (Oxyria digyna) by conducting a phylogeographic analysis of plastid and nuclear gene DNA variation.

We provide evidence for an origin of this species in the Qinghai‐Tibet Plateau of southwestern China, followed by migration into Russia c. 11 million yr ago (Ma), eastwards into North America by c. 4 Ma, and westwards into Western Europe by c. 1.96 Ma. Thereafter, the species attained a circumarctic distribution by colonizing Greenland from both sides of the Atlantic Ocean.

Following the arrival of the species in North America and Europe, population sizes appear to have increased and then stabilized there over the last 1 million yr. However, in Greenland a marked reduction followed by an expansion in population size is indicated to have occurred during the Pleistocene.
Human leukocyte antigens and genetic susceptibility to lymphoma
Authors: McAulay K. A. , Jarrett R. F. 
Published: July 2015

AbstractFamilial aggregation, coupled with ethnic variation in incidence, suggests that inherited susceptibility plays a role in the development of lymphoma, and the search for genetic risk factors has highlighted the contribution of the human leukocyte antigen (HLA) complex. In a landmark study published almost 50 years ago, Hodgkin lymphoma (HL) was the first disease to be associated with HLA variation. It is now clear that Epstein‐Barr virus (EBV)‐positive and ‐negative HL are strongly associated with specific HLA polymorphisms but these differ by EBV status of the tumours. HLA class I alleles are consistently associated with EBV‐positive HL while a polymorphism in HLA class II is the strongest predictor of risk of EBV‐negative HL. Recent investigations, particularly genome‐wide association studies (GWAS), have also revealed associations between HLA and common types of non‐Hodgkin lymphoma (NHL). Follicular lymphoma is strongly associated with two distinct haplotypes in HLA class II whereas diffuse large B‐cell lymphoma is most strongly associated with HLA‐B*08. Although chronic lymphocytic leukaemia is associated with variation in HLA class II, the strongest signals in GWAS are from non‐HLA polymorphisms, suggesting that inherited susceptibility is explained by co‐inheritance of multiple low risk variants. Associations between B‐cell derived lymphoma and HLA variation suggest that antigen presentation, or lack of, plays an important role in disease pathogenesis but the precise mechanisms have yet to be elucidated.
CLAUSA restricts tomato leaf morphogenesis and GOBLET expression
Authors: Bar Maya , Ben‐Herzel Ori , Kohay Hagay , Shtein Ilana , Ori Naomi 
Published: July 2015

SummaryLeaf morphogenesis and differentiation are highly flexible processes. The development of compound leaves is characterized by an extended morphogenesis stage compared with that of simple leaves. The tomato mutant clausa (clau) possesses extremely elaborate compound leaves. Here we show that this elaboration is generated by further extension of the morphogenetic window, partly via the activity of ectopic meristems present on clau leaves. Further, we propose that CLAU might negatively affect expression of the NAM/CUC gene GOBLET (GOB), an important modulator of compound‐leaf development, as GOB expression is elevated in clau mutants and reducing GOB expression suppresses the clau phenotype. Expression of GOB is also elevated in the compound leaf mutant lyrate (lyr), and the remarkable enhancement of the clau phenotype by lyr suggests that clau and lyr affect leaf development and GOB in different pathways.
Cardiac malposition (ectopia cordis) in a cat
Authors: Lopez Melinda M. , Kuzma Alan B. , Margiocco Marco L. , Cheng Teresa , Enberg Trevor B. , Head Laurie 
Published: July 2015

AbstractObjectiveTo describe a case of cardiac malposition in a cat, and the successful management of the anomaly.Case DescriptionA 2‐year‐old male neutered male British Shorthair cat weighing 7.58 kg was referred for bicavitary effusion. Ultrasonography and echocardiography demonstrated displacement of the heart into the abdomen through a diaphragmatic defect. Clinical signs of right‐sided congestive heart failure were attributed to mechanical restriction of diastolic function by a constrictive segment of fibrous pericardium and to impaired venous return due to a kink in the caudal vena cava. Surgical repositioning of the heart into the thoracic cavity and a subtotal pericardectomy were performed, and the diaphragmatic defect was repaired. The patient recovered well postoperatively.New or Unique Information ProvidedThe diagnosis and management of cardiac malposition has not been previously described in cats. With timely diagnosis and surgical intervention, a favorable outcome is possible.
Mathematical model for predicting sludge accumulation in septic tanks based on operational parameters: effect of hydraulic mixing
Authors: Al Momani Fares 
Published: July 2015

AbstractReporting sludge accumulation in septic tanks as a constant rate is no longer accepted, and there is a need for a model that predicts sludge accumulation in septic tanks taking into consideration the continuous consumption of sewage solids by microorganisms. This study presents the development of mathematical model that predicts sludge accumulation in septic tanks and to investigate the effect of mixing on solids digestion. The mathematical model takes into consideration the effect of different operational parameters (influent and effluent) as well as the bacterial kinetics in predicting the sludge accumulation in septic tanks. The model predicts with R2 > 0.88 the sludge accumulation rate in standard septic tank calculated by empirical models developed by Weibel in 1955, and by Bounds in 1995. The model was also used to estimate the typical pump‐out interval for different tank volumes and the sludge accumulation inside septic tanks at different operational parameters.
Postdiagnostic Statin Use and the Risk of Lethal Prostate Cancer in the Health Professionals Follow-up Study
Authors: Chan June M. , Kenfield Stacey A. , Paciorek Alan , Platz Elizabeth A. , Giovannucci Edward L. , Stampfer Meir J. 
Published: July 2015

Abstract
Background: Observational studies suggest potential chemopreventive benefits of statins on prostate cancer outcomes, but data on the impact of postdiagnostic use are sparse.
Methods: We examined the association of postdiagnostic statin use and risk of lethal prostate cancer (metastases or prostate cancer death, N = 242) among 3,949 men diagnosed with localized prostate cancer from the Health Professionals Follow-Up Study between 1992 and 2008 and followed through 2010 (33,302 person years). We used Cox proportional hazards regression models to estimate relative risks and 95% confidence intervals (CI), adjusting for age, time period, time from diagnosis to questionnaire, body mass index, vigorous physical activity, smoking, aspirin use, clinical stage, PSA at diagnosis, Gleason score, primary treatment, and comorbidities.
Results: We found no statistically significant association between postdiagnostic current use of statins or duration of statin usage and the outcome of lethal prostate cancer [N = 242 cases; multivariate HR = 0.97 (95% CI, 0.72–1.31) for current use yes/no; HR = 0.85 (95% CI, 0.59–1.22) for 1 to 5 years of use, 0.96 (95% CI, 0.66–1.38) for 6+ years of use vs. never use].
Conclusions: We observed little evidence that statin usage after diagnosis of localized prostate cancer reduces risk of progression to metastatic disease or prostate cancer–specific death.
Impact: These results do not support statins as a chemopreventive agent for prostate cancer progression. Cancer Epidemiol Biomarkers Prev; 24(10); 1638–40. ©2015 AACR.
Rudeness at Work: Impulse over Restraint
Authors: Johnson Pamela R. , Indvik Julie 
Published: July 2015

Office rudeness is on the rise. Catcalls, disparaging remarks whispered behind closed doors, and raging e-mails are testaments to the fact that many of us are overworked and stressed out. While much has been written about violence in the workplace, it is often the small and subtle egregious acts, the little incivilities, that take the largest toll. Rudeness is at the low end of the continuum of workplace abuse; workplace rudeness isn't violence or harassment or even open conflict, although it can build up to any of those things. Links between the work environment and indicators of employee loyalty, commitment, and productivity show this is not a “fluff” issue. In addition, a recent study on workplace incivility reveals that rude employees and managers can cost a company millions of dollars a year. This paper will give some background on rudeness at work, identify the kinds and causes of poor behavior, enumerate the costs to organizations, and discuss what employers can do to reduce rudeness at work.