bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modeling Decision-Making Under Uncertainty with Qualitative Outcomes
Authors: Korem Nachshon , Or Duek , Rounan Jia , Wertheimer Emily , Metviner Sierra , Grubb Michael , Levy Ifat 
Published: August 2024

AbstractModeling decision-making under uncertainty typically relies on quantitative outcomes. Many decisions, however, are qualitative in nature, posing problems for traditional models. Here, we aimed to model uncertainty attitudes in decisions with qualitative outcomes. Participants made choices between certain outcomes and the chance for more favorable outcomes in quantitative (monetary) and qualitative (medical) modalities. Using computational modeling, we estimated the values participants assigned to qualitative outcomes and compared uncertainty attitudes across domains. Our model provided a good fit for the data, including quantitative estimates for qualitative outcomes. The model outperformed a utility function in quantitative decisions. Additionally, we found an association between ambiguity attitudes across domains. Results were replicated in an independent sample. We demonstrate the ability to extract quantitative measures from qualitative outcomes, leading to better estimation of subjective values. This allows for the characterization of individual behavior traits under a wide range of conditions.Author SummaryIn the current study, we explored how people make decisions when the outcomes aren’t easily measured in numbers, such as in medical choices. Traditional mathematical models, which rely on numerical data, often fall short in these situations, leading to a gap in understanding how people evaluate these qualitative outcomes. Using hierarchical Bayesian modeling, we developed a model that bridges this gap by translating qualitative outcomes into individualized quantitative values, enabling us to better understand the underlying decision-making processes. Our model not only provides a better fit to real-world data than existing models with qualitative or quantitative outcomes but also allows for meaningful comparisons of how people handle uncertainty across different decision-making scenarios. This approach opens new doors for studying decision-making in areas where traditional methods struggle, offering a more nuanced view of human behavior in complex situations.
The VVBlue assay: a plate-readable, dye exclusion-based cell viability assay for the toxicological testing of chemicals
Authors: Vitipon Marianne , Akingbagbohun Esther , Rabilloud Thierry 
Published: August 2024

AbstractA viability test for in vitro cultures, based on the intake of the textile dye Alphazurine A by dead cells and its exclusion by viable cells, is described. This test uses the affinity of Alphazurine A for proteins, so that the dye is retained in dead cells even after rinsing, while its anionic character prevents it from entering live cells. This feature makes this dye exclusion test amenable to a reading in a plate format. The Alphazurine viability test provides an indicator of the absolute number of dead cells present in the culture well. To reach a cell viability index, a “dead cells” control (e.g. cells killed with ethanol) must be added. We also describe a double viability test, which first uses the Alphazurine assay to provide the number of dead cells then a crystal violet assay to provide an index of the number of cells present in the plate. This double test provides a complete appraisal of the situation in the cell culture wells, and has been compared to other viability tests such as propidium iodide exclusion or tetrazolium reduction. Its performances to study the toxicity of substances such as pigments are also established, and allowed us to publish the first public toxicological data on the recently described Pigment Blue 86.
Whole-embryo Spatial Transcriptomics at Subcellular Resolution from Gastrulation to Organogenesis
Authors: Wan Yinan , El Kholtei Jakob , Jenie Ignatius , Colomer-Rosell Mariona , Liu Jialin , Acedo Joaquin Navajas , Du Lucia Y. , Codina-Tobias Mireia , Wang Mengfan , Sawh Ahilya , Lin Edward , Chuang Tzy-Harn , Mango Susan E. , Yu Guoqiang , Bintu Bogdan , Schier Alexander F. 
Published: August 2024

AbstractSpatiotemporal patterns of gene expression underlie embryogenesis. Despite progress in single-cell genomics, mapping these patterns across whole embryos with comprehensive gene coverage and at high resolution has remained elusive. Here, we introduce awhole-embryo imaging platform usingmultiplexederror-robust fluorescent in-situhybridization (weMERFISH). We quantified the expression of 495 genes in whole-mount zebrafish embryos at subcellular resolution. Integration with single-cell multiomics data generated an atlas detailing the expression of 25,872 genes and the accessibility of 294,954 chromatin regions, explorable with an online interface MERFISHEYES (beta version). We found that temporal gene expression aligns with cellular maturation and morphogenetic movements, diverse expression patterns correspond to composites of tissue-specific accessible elements, and changes in gene expression generate sharp boundaries during gastrulation. These results establish a novel approach for whole-organism spatial transcriptomics, provide a comprehensive spatially resolved atlas of gene expression and chromatin accessibility, and reveal the diversity, precision and emergence of embryonic patterns.
A non-tethering role for the Drosophila Pol θ linker domain in promoting damage resolution
Authors: Blanch Justin R. , Krishnamurthy Manan , McVey Mitch 
Published: August 2024

AbstractDNA polymerase theta (Pol θ) is an error-prone translesion polymerase that becomes crucial for DNA double-strand break repair when cells are deficient in homologous recombination or non-homologous end joining. In some organisms, Pol θ also promotes tolerance of DNA interstrand crosslinks. Due to its importance in DNA damage tolerance, Pol θ is an emerging target for treatment of cancer and disease. Prior work has characterized the functions of the Pol θ helicase-like and polymerase domains, but the roles of the linker domain are largely unknown. Here, we show that theDrosophila melanogasterPol θ linker domain promotes egg development and is required for tolerance of DNA double-strand breaks and interstrand crosslinks. While a linker domain with scrambled amino acid residues is sufficient for DNA repair, replacement of the linker with part of theHomo sapiensPol θ linker or a disordered region from the FUS RNA-binding protein does not restore function. These results demonstrate that the linker domain is not simply a random tether between the helicase-like and polymerase domains. Furthermore, they suggest that intrinsic amino acid residue properties, rather than protein interaction motifs, are more critical for Pol θ linker functions in DNA repair.
Behavioral screening defines three molecular Parkinsonism subgroups inDrosophila
Authors: Kaempf Natalie , Valadas Jorge S. , Robberechts Pieter , Schoovaerts Nils , Praschberger Roman , Ortega Antonio , Kilic Ayse , Chabot Dries , Pech Uli , Kuenen Sabine , Vilain Sven , Baz El-Sayed , Singh Jeevanjot , Davis Jesse , Liu Sha , Verstreken Patrik 
Published: August 2024

AbstractParkinsonism is defined by motor dysfunction, but the specific upstream molecular causes of these clinical symptoms can vary widely. We hypothesize that these causes converge onto a limited number of core cellular pathways. To investigate this, we created a new collection of 24 genetically very well-controlled animal models of familial forms of parkinsonism. Using unbiased behavioral screening and machine learning we identified three clusters of mutants that converge on (1) mitochondrial function; (2) retromer/vesicle trafficking; and (3) proteostasis/autophagy. Genes within each cluster have a similar genetic interaction profile and compounds that target specific molecular pathways ameliorate dopaminergic neuron dysfunction in a cluster-specific manner. This suggests that familial parkinsonism can be stratified into three broad functional groups and our findings pave the way for targeted biomarker discovery and drug development.
Analysing drivers of worldwide tidal wetland change
Authors: Perrodin Lucie , Navarro Alejandro , Toor Maren , Canto Robert , Becker Madison , Dong Yanzhu , Worthington Thomas , Murray Nicholas J. 
Published: August 2024

AbstractTidal wetlands are dynamic coastal ecosystems that can change in extent in response to a broad range of change drivers. We use high spatial resolution satellite imagery to estimate the relative influence of 18 classes of change drivers on observed tidal wetland gains and losses from 1999 to 2019, differentiating direct drivers as those observable at the site of ecosystem change, and indirect drivers as broader processes that influence changes without being directly visible. We developed a random sample of 2823 change detections from a global dataset of tidal wetland change and allocated each change event to driver classes using high-resolution time-series imagery. We identified that indirect drivers were the most widespread type of driver of tidal wetland change (70.9%), with flooding being the predominant driver for losses (47.5%) and unknown natural processes of change for gains (62.7%). Drivers often associated with climate change were evident in interpretations of wetland drivers, with increases in flooded area and reductions in vegetation cover suggesting the effects of relative sea level rise on tidal wetlands are observable in many areas. Our temporal analysis revealed that over 20 years, indirect drivers consistently contributed to larger proportions of gains and losses compared to direct drivers. Asia was the only continent where direct drivers of loss, such as agriculture (22.9%) and aquaculture (17.1%), outweighed indirect drivers, providing further evidence of the widespread transformation of Asia’s natural coastal ecosystems to anthropogenic shorelines. Globally, coastal land reclamations were mostly observed in mangrove ecosystems, where more than half of the observed losses were of anthropogenic origin. The most observed direct drivers of gains were altered land management and restoration, but none of them contributed to more than 5% of the total gains over 20 years. Our findings suggest a need for efficient conservation measures that allow the dynamic processes that characterise coastal ecosystems to persist, while simultaneously reducing the worldwide impact of direct human activities.
A non-canonical CDK, Pho85 regulates the restart of the cell-cycle following stress
Authors: Jin Natsuko , Jin Yui , Oikawa Yu , Nakano Akihiko , Ohsumi Yoshinori , Weisman Lois S. 
Published: August 2024

AbstractEnvironmental stress induces an arrest of the cell cycle. Thus, release from this arrest is essential for cell survival. The cell-cycle-arrest occurs via the down regulation of the cyclins that drive the main cyclin dependent kinase, CDK1/Cdc28. However, it was not clear how cells escape this potentially fatal arrest. Here we show that prior to the restoration of CDK1/Cdc28 cyclins, a non-canonical CDK, Pho85, initiates a cascade to restart the cell cycle. We demonstrate that following stress, Pho85 phosphorylates the Sch9 kinase, which in turn directly phosphorylates the transcriptional inhibitor Whi5, the yeast analog of RB1/retinoblastoma, and a CDK1 target. This promotes Whi5 translocation from the nucleus, and the release of the stress-induced arrest at G1phase. In addition, we find that in parallel with Pho85, CDK1/Cdc28 also plays a role in the control of Whi5. Together, these findings provide insights into how cells re-enter the cell cycle during recovery from stress and reveal that a non-canonical CDK and cyclin takes on essential roles and acts via a pathway that functions in parallel with CDK1/Cdc28.
Computational modeling of left ventricular flow using PC-CMR-derived four-dimensional wall motion
Authors: Peighambari Seyed Babak , Mukherjee Tanmay , Mendiola Emilio A. , Darwish Amr , Timmins Lucas H. , Pettigrew Roderic I. , Shah Dipan J. , Avazmohammadi Reza 
Published: August 2024

ABSTRACTIntracardiac hemodynamics plays a crucial role in the onset and development of cardiac and valvular diseases. Simulations of blood flow in the left ventricle (LV) have provided valuable insight into assessing LV hemodynamics. While fully coupled fluid-solid modelings of the LV remain challenging due to the complex passive-active behavior of the LV wall myocardium, the integration of imaging-driven quantification of structural motion with computational fluid dynamics (CFD) modeling in the LV holds the promise of feasible and clinically translatable characterization of patient-specific LV hemodynamics. In this study, we propose to integrate two magnetic resonance imaging (MRI) modalities with the moving-boundary CFD method to characterize intracardiac LV hemodynamics. Our method uses the standard cine cardiac magnetic resonance (CMR) images to estimate four-dimensional myocardial motion, eliminating the need for involved myocardial material modeling to capture LV wall behavior. In conjunction with CMR, phase contrast-MRI (PC-MRI) was used to measure temporal blood inflow rates at the mitral orifice, serving as an additional boundary condition. Flow patterns, including velocity streamlines, vortex rings, and kinetic energy, were characterized and compared to the available data. Moreover, relationships between LV wall kinematic markers and flow characteristics were determined without myocardial material modeling and using a non-rigid image registration (NRIR) method. The fidelity of the simulation was quantitatively evaluated by validating the flow rate at the aortic outflow tract against respective PC-MRI measures. The proposed methodology offers a novel and feasible toolset that works with standard PC-CMR protocols to improve the clinical assessment of LV characteristics in prognostic studies and surgical planning.
Survival of hepatocytes from executioner caspase activation promotes liver regeneration by enhancing JAK/STAT3 activity
Authors: Cao Zhiyuan , Qin Lining , Liu Kaixuan , Yao Chen , Li Enhong , Hao Xiaoyu , Wang Molin , Jiang Baichun , Zou Yongxin , Hu Huili , Liu Qiao , Shao Changshun , Gong Yaoqin , Sun Gongping 
Published: August 2024

AbstractActivation of executioner caspases, which is a key step in the apoptotic process, has been reported to promote tissue regeneration by sending pro-proliferation signals to the surrounding cells. However, whether executioner caspase activation (ECA) has cell-autonomous effect on tissue regeneration is not clear. Here, by generating transgenic mice carrying a lineage tracing system for cells that have experienced ECA, we demonstrate that transient ECA occurs in hepatocytes during liver regeneration after partial hepatectomy (PHx) or carbon tetrachloride (CCl4) treatment. Instead of committing apoptotic cell death, the majority of hepatocytes with ECA survive and proliferate to contribute to liver regeneration. Interestingly, inhibition of ECA in livers results in reduced hepatocyte proliferation and impaired regeneration, whereas increasing ECA to a level sufficient to kill hepatocytes also impedes regeneration, suggesting that ECA needs to be precisely controlled during liver regeneration. Mechanistic studies show that ECA promotes hepatocyte proliferation during regeneration through enhancing JAK/STAT3 activity. Our work reveals an essential role of survival of hepatocytes from ECA in liver regeneration.
Young consumers' online experiential consumption behavior of foreign contemporary music: generational and gender differences in the refined stimulus-organism-response theory
Authors: Kim Wooyang , An Hyun Sang , Hantula Donald A. , Di Benedetto Anthony 
Published: August 2024

PurposeThis study aims to examine the younger generations’ experiential consumption of foreign contemporary music online (i.e. digital music streaming services) by generation and gender in the US market.Design/methodology/approachThe author proposes a sequential experiential consumption model by applying Jacoby’s refined stimulus-organism-response (S-O-R) theory to better understand the experiential sequences in foreign music consumption among young generations in the US market. The proposed model, using structural equation modeling (SEM), examines a cognitive permeable role and a hierarchical affective mediating role. Also, moderating roles of generation and gender are simultaneously tested in overall and specific causal relationships.FindingsThe refined S-O-R framework is superior to a linear one in better understanding young consumers’ online experiential foreign music consumption behavior. Moreover, hierarchical sequenced affective organismic behavior is crucial to enhance young consumers’ online music consumption experiences to regulate subsequent behavioral responses. Furthermore, gender differences but no generational differences exist in the experiential consumption process among young consumers. Nevertheless, the strength of S-O-R factors affecting experiential consumption seems idiosyncratic simultaneously in gender and generation.Practical implicationsThe study suggests foreign music streaming services boost profitability by focusing on young consumers' psychological ownership and tailored experiences, encouraging a shift from freemium to premium subscriptions. Also, the findings recommend adopting phygital experiences using technologies like AR, VR and MR to enhance engagement and create unique, emotionally resonant experiences for young consumers, thus fostering a more profitable business model.Originality/valueThe authors address under-researched topics relevant to young generations by applying Jacoby’s refined S-O-R framework to foreign music consumption through online streaming. This approach delves into a lesser-explored consumer behavior framework, highlighting young generations’ musical trends. The model reveals cognitive and affective roles, offering advantages over traditional linear S-O-R models. It also uniquely incorporates the moderating effects of generation and gender in music consumption studies, addressing a gap in music-related studies.
Navigating negative events: the role of online destination brand experience in tourists' travel decisions
Authors: Shah Adnan Muhammad , Qayyum Abdul , Shah Mahmood , Jamil Raja Ahmed , Lee KangYoon 
Published: August 2024

PurposeThis study addresses tourists' post-consumption perspectives on the impact of online destination experiences and animosity on travel decisions. Developing a framework based on the stimulus-organism-response (SOR) theory, we examine the previously unexplored relationship between post-negative events, online destination brand experience (ODBE), tourists' animosity and destination boycott intentions within the domestic tourism context.Design/methodology/approachData from 355 actively engaged domestic travelers in Pakistan who follow destination social media pages (i.e. Instagram and Facebook) was analyzed using structural equation modeling.FindingsThe findings reveal that post-negative events ODBE significantly stimulate tourists' animosity, which in turn drives destination boycott intentions. The ODBE indirectly affects boycott intentions through animosity, acting as a partial mediator. The analysis highlights the significance of the users' prior experience levels (novice vs experienced). Multigroup analysis shows that novice visitors are more sensitive to negative online experiences, resulting in stronger animosity than experienced visitors. Animosity significantly drives boycott intentions, particularly among experienced visitors.Originality/valueThis study’s novelty lies in its comprehensive examination of post-negative events, focusing on how the ODBE influences tourists' negative emotions and boycott intentions. These findings offer valuable insights for tourism researchers and destination marketers, underscoring the importance of optimizing post-service failure ODBE strategies for brand repair, online reputation management, digital marketing innovation and customized service recovery to mitigate the impact of negative events.
The effects of quality perception and multisensory perception on purchase intention when consumers shop online
Authors: Han Bo , Li Pei , Tan Xiaoqin 
Published: August 2024

PurposeHow to reduce uncertainty in clothing online purchasing has become a primary concern for consumers. This study aims to investigate the effects of quality perception and multisensory perception on consumer online clothing purchase intention and to explore the mediating effect of trust.Design/methodology/approachThis study was conducted with consumers who have online clothing shopping experiences. A total of 539 valid samples were analyzed by bootstrap and structural equation model.FindingsThe result of this research revealed that perceived information quality, perceived service quality, perceived haptic imagery and perceived visual-audio cues have a significant influence on purchase intention. Additionally, consumers’ trust is positively correlated with purchase intention. Trust mediates the relationship between the perceptions (perceived information quality, perceived service quality, perceived haptic imagery and perceived visual-audio cues) and purchase intention, irrespectively.Originality/valueTo investigate the effects of multisensory perception on purchase intention, perceived haptic imagery is adopted as a variable in this study. This study investigates the mediating role of trust between multisensory perception and purchase intention. The findings of this study can enrich Stimulus-Organism-Response (S-O-R) framework and Information System (IS) success model in the field of clothing online shopping.
Investigating women’s work experience in hospitality with the job demands-resources theory
Authors: Zhong YunYing (Susan) 
Published: August 2024

PurposeThis study aims to investigate the work experience of women in hospitality with the job demands-resources (JD-R) theory. By focusing on job resources, job demands (emotional and physical), work engagement, emotional exhaustion and turnover intention, this study examines (1) female workers’ perceptions of these variables; (2) how race, age and job positions affect perceptions and (3) structural relationships among these variables.Design/methodology/approachThe study collected 412 responses from American female hospitality workers via an online survey. Descriptive statistics and independent T-tests were conducted using SPSS to analyze professional experiences and differences based on individual characteristics. Path analysis was conducted using Amos 28 to assess the structural relationships among variables.FindingsFemale hospitality workers generally feel engaged, experience low emotional exhaustion and have low turnover intention. Younger or front-line women reported higher emotional exhaustion, lower engagement and greater likelihood to quit. Unexpectedly, emotional resources do not significantly affect work engagement, but physical resources increase it.Practical implicationsThe study provides directions to establish specific well-being and organizational support initiatives to retain female hospitality workers.Originality/valueThis study offers fresh insights into the JD-R theory by examining the experiences of American female hospitality employees using a non-comparative lens. Although existing literature highlights women’s unfavorable positions relative to male counterparts, this study reveals rather positive perspectives. Additionally, it presents a dual psychological process of how job resources and demands affect women’s work experience and the varying impacts of job demands on work engagement.