bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on the mechanism of KIF2C/PLK1 in the growth and metastasis of oral squamous cell carcinoma
Authors: Wang Chenfei , Su Yuyuan , Shi Jinlong , Feng Guijuan 
Published: July 2024

Abstract

Oral squamous cell carcinoma (OSCC) is a prevalent malignant tumor characterized by challenging early symptom detection, rapid growth, extensive infiltration, and frequent lymph node metastasis, resulting in a generally unfavorable prognosis. Given the heterogeneity and high metastatic potential of OSCC, there is a need for more effective therapeutic targets. This study aims to investigate the impact of kinesin family member 2C (KIF2C) and Polo-like protein kinase 1 (PLK1) on OSCC progression and their potential interaction. Through the utilization of immunohistochemistry and western blot experiments, it was determined that the expressions of KIF2C and PLK1 were significantly upregulated in human oral cancer tissues and OSCC cell lines (SCC9, SCC25, Cal27). Subsequently, an OSCC cell was selected for analysis based on the detection of KIF2C expression (qPCR, WB) in the OSCC cell line. Following transfection with KIF2C and PLK1 interference vectors, the proliferation, migration, and invasion of OSCC cells were assessed using the CCK-8 method, EdU proliferation experiment, wound closure method, and Transwell method. Flow cytometry (FCM) and western blot techniques were utilized to assess alterations in apoptosis, angiogenesis, and epithelial-mesenchymal transition (EMT) in OSCC cells. Our findings indicate that KIF2C influences the proliferation, invasion, and metastasis of oral cancer cells, suggesting a potential association between KIF2C and PLK1. Specifically, KIF2C was found to enhance EMT, matrix degradation, and angiogenesis in OSCC cells by up-regulating PLK1. Subsequent flow cytometry analysis revealed that KIF2C and PLK1 suppressed apoptosis in cancer cells and induced proliferation in the S phase, thereby promoting cell proliferation. The association between the expression levels of KIF2C and PLK1 and the development and advancement of OSCC was confirmed, suggesting their potential utility as diagnostic and prognostic markers for OSCC.
Discovery of the Widespread Site-Specific Single-Stranded Nuclease Family Ssn
Authors: Veyrier Frédéric , Chenal Martin , Rivera-Millot Alex , Harrisson Luke , Khairalla Ahmed , Nieves Cecilia , Bernet Eve , Esmaili Mansoor , Belkhir Manel , Perreault Jonathan 
Published: July 2024

Abstract

Site-specific endonucleases that exclusively cut single-stranded DNA have hitherto never been described and constitute a barrier to the development of ssDNA-based technologies. Here, we identify and characterize one such novel family of widely distributed site-specific single-stranded nucleases (Ssn) exhibiting unique ssDNA cleavage properties. This family is a member of the GIY-YIG nuclease superfamily that encompasses restriction enzymes, homing endonucleases, and transposases involved in important biological processes. Unlike other families of the GIY-YIG superfamily that typically possess additional functional domains that dictate their various specificities, Ssn are small proteins only consisting of the GIY-YIG domain. By first comprehensively studying the Ssn homolog from Neisseria meningitidis, we demonstrate that it interacts specifically with a sequence element (called NTS) present in hundreds of copies and surrounding important genes in pathogenic Neisseria. In this species, NTS/Ssn interactions modulate horizontal gene transfers through natural transformation and thus constitute a novel mechanism shaping genome dynamics. We further identified thousands of Ssn homologs throughout the bacterial kingdom and demonstrate, in vitro, a range of Ssn nuclease specificities for their corresponding NTS. We demonstrate proofs of concept for applications including ssDNA detection and digestion of ssDNA from Rolling Circle Amplification. This discovery and its applications set the stage for the development of innovative ssDNA-based molecular tools and technologies.
Identification of prognostic biomarkers for endometrioid endometrial carcinoma based on the miRNA and mRNA co‐expression network regulated by estradiol
Authors: Jin Li , Xie Qiu , Huang Junting , Xie Yuan 
Published: July 2024

Abstract

Background: Endometrioid endometrial carcinoma (EEC), an estradiol-related disease, remains a serious health threat to women because of its high incidence and trend of rejuvenation. Accumulating evidence has highlighted that microRNAs (miRNAs) and messenger RNAs (mRNAs) play important roles in various biological processes involved in the pathogenesis of EEC. This study aimed to identify the potential prognostic biomarkers associated with EEC regulated by estradiol.
Results: Four estradiol-related DEGs were associated with prognosis, and 235 overlapping target DEGs were screened and incorporated into the functional enrichment analysis and protein–protein interaction network visualization studies. Additionally, SACS and GPR157were identified as potential biomarkers for EEC prognosis through survival analyses. Furthermore, single-cell transcriptome data were analyzed to show changesin gene expression levels in specific cell types.
Conclusions: This study demonstrates that miR-142-5p–SACSand miR-30a-5p–GPR157, which are regulated by estradiol, may hold promise as diagnostic and prognostic biomarkers and novel therapeutic targets for EEC.
Predictors of Work-Related Violence Against Nurses Working at a Tertiary Hospital in Kisumu, Kenya.
Authors: Atogo Anne Owira , Nyaberi Zablon , Arudo John 
Published: July 2024

Abstract

Background Workplace violence (WPV) is any act in which a person is abused, threatened, intimidated or assaulted in their work environment. It could involve physical, verbal or written threatening behaviour or physical attacks. Workplace violence against nurses is increasingly becoming a major problem globally. Because of the nature of their work, nurses are at increased risk of workplace violence.Objective To assess workplace violence against nurses at Jaramogi Oginga Odinga Teaching and Referral Hospital.Materials and methods It employed a cross-sectional study design among 184 nurses randomly selected from Jaramogi Oginga Odinga Teaching and Referral Hospital in Kisumu-Kenya. Data was collected using a self-administered questionnaire that asked questions regarding their sociodemographic characteristics, nature of their work, experience with workplace violence and institutional factors that could be associated with workplace violence. The data obtained was analysed descriptively (in the form of frequencies and proportions for categorical variable and inferentially as mean with corresponding standard deviation) and inferentially. Pearson chi-square test was adopted to assess statistically significant association between predictors of WPV and violence occurrence. Odds ratios were computed at 95% confidence interval.Results This study enrolled 184 nurses with a mean age of 37 (± 9.5) years, of whom 62% were female. Majority (88%; n = 162) of the nurses enrolled were at the operational level with 2 (1.1%) being supervisors and 20 (10.9%) being in senior management. Workplace violence was reported by 70% of the respondents, with 41.8% of them saying they were verbally abused. Among those physically abused, 71.8% were assaulted by the relatives of the patients with 28.2% being injured because of the incident. Bullying by co-workers was noted among 24 (13.0%) of the respondents while 41 (22.3%) reported sexual harassment. 96.2% had not received any training on WPV prevention in the last twelve months. Working in the general surgery department (p < 0.001) and lack of safety measures (p = 0.020) predicted WPV.Conclusion the study reports that working in general surgical wards and lack of institutional measures against violence are the major predictors of workplace violence among nurses. There is need to understand institutional processes, procedures and operations that reduce the likelihood of workplace violence in other hospital departments and apply these findings to the general surgery unit where there was a higher prevalence of workplace violence.
PERAN DINAS EKONOMI KREATIF DAN UKM DALAM PEMBERDAYAAN UMKM PEREMPUAN DI KABUPATEN SLEMAN YOGYAKARTA
Authors: Nisa Kanita Khoirun , Wahyuni Titi , Budita Ayla Karina 
Published: July 2024

Abstrak: Aplikasi ekonomi kreatif telah menjadi trendsetter di banyak negara di dunia. Konsep creative economy menjadi terobosan bisnis baru yang memadukan unsur kreativitas dan pembaruan dalam pembangunan ekonomi. Peneliti terdahulu telah banyak yang melakukan penelitian di sektor UMKM dengan memperhatikan fenomena sektor ekonomi kreatif, namun kajian mendalam tentang UMKM masih kurang. Kajian ini bermaksud untuk menggali fungsi dari Dinas Ekonomi Kreatif dalam Pemberdayaan UMKM perempuan di Kabupaten Sleman. Penelitian menggunakan deskriptif kualitatif, data dikumpulkan secara observasi, wawancara, dan dokumentasi telah digunakan pada studi ini. Hasil studi menginformasikan telah ada dan terus berlanjut upaya pemberdayaan yang dilakukan oleh dinas ekonomi kepada para pelaku UMKM di Kabupaten Sleman. Bentuk pemberdayaan yang dilakukan di antaranya pendampingan dan sosialisasi.
Kata Kunci: Pemberdayaan; UMKM; Pendampingan

Abstract: The application of the creative economy has become trendsetters in many countries around the world. The creative economy concept is a new business breakthrough that combines elements of creativity and innovation in economic development. Many previous researchers have conducted research on the MSME sector by paying attention to the phenomenon of the creative economy sector, but in-depth studies on MSMEs are still lacking. This study intends to explore the function of the Creative Economy Service in empowering women's MSMEs in Sleman Regency. This research used descriptive research, data was collected through observation, interviews, and documentation. The study results indicate that there have been and are continuing empowerment carried out by the economic service for MSME actors in the Sleman Regency. Forms of empowerment carried out include mentoring and socialization.
Keyword: Empowerment; MSMEs; Mentoring
Association of genetic variants of hemostatic system genes with venous thrombosis in children born to mothers with a burdened obstetric and gynecological history
Authors: Perevezentsev O. A. , Mamedov I. S. , Burtsev D. V. 
Published: July 2024

Thrombophilias are hereditary and acquired conditions characterized by an excessive tendency of the body to form thrombi in the blood vessels. Idiopathic venous thrombosis can often occur in childhood and can also be associated with certain genetic variants of hereditary predisposition to thrombophilia.Purpose. To analyze the association of 8 genetic variants (F2 20210G>A, F5 1691G>A, F7 10976G>A, F13 G>T, ITGA2 807C>T, ITGB3 1565 T>C, PAI-1–675 5G>4G) with venous thrombosis in children born to mothers with a burdened obstetric and gynecological history.Material and methods. The patient group included 322 children aged 7 to 14 years (average age 9.3 years), who had a history of episodes of venous thrombosis of various locations, born to mothers with obstetric and gynecological history. The comparison group included 159 healthy children also aged from 7 to 14 years (average age 9.5 years), who did not have a history of episodes of venous thrombosis and who were also born to mothers with obstetric and gynecological history. Molecular genetic analysis was carried out using real-time PCR with automatic analysis of melting curves.Results. Based on the results of an analysis of the association of genetic variants with venous thrombosis in children born to mothers with obstetric and gynecological history, a connection with this pathology was established for genetic variants F5 1691G>A (genotype GA+AA, OR=3.33, 95% CI: 1.19 — 9.36), ITGA2 807C >T (TT genotype (OR=1.92, 95% CI:1.20 — 3.06) and heterozygous CT (OR=1.46, 95% CI: 1.10 — 1.93)) and ITGB3 1565 T>C (CC genotype (OR=2.77 95% CI:1.08 — 7.02) and TC (OR=1.40, 95% CI: 1.07 — 1.83)).Conclusion. Thus, we established an association of 3 genetic variants (Leiden mutation, ITGA2 807C>T and ITGB3 1565 T>C) with venous thrombosis in children born to mothers with obstetric and gynecological history.
Hydraulic investigation of flows at high-head overflow spillway with multiple aerators: a physical and numerical study of Mohmand Dam, Pakistan
Authors: Ali Syed Abbas , Khan Noor Muhammad , Sarwar Muhammad Kaleem , Zaffar Muhammad Waqas , Tariq Atiq Ur Rehman , Ullah Ubaid , Abbas Yasir 
Published: July 2024

ABSTRACT
A spillway is the essential part of the dam body, which releases surplus flows. At higher floods, the spillway operates at high heads, which results in high flow velocities along the chute and may cause negative pressures and cavitation. Therefore, to minimize such issues, aerators are provided along the spillway's chutes. This study aims to analyze the performance of the high-head overflow spillway of Mohmand Dam, Pakistan, having a steep chute of 32° with multiple aerators. Based on Froude's law of similitude, the physical model study was carried out at Irrigation Research Institute, Nandipur, on a scale of 1:60, while FLOW-3D numerical models were used to compare different hydraulic parameters, i.e., flow depth, velocity and pressure. The numerical models were validated with the results of a physical model, which were found in an acceptable range (i.e., 4.93%), and the hydraulic performance of two aerators was evaluated at different discharges. The models indicated negative pressures inside the aerator cavity, which allowed the suction of air to the lower nappe. The maximum air entrainment at the first aerator was about 8.5%. The results also showed that air entrainment to the lower nappe decreased when discharge was increased, whereas the maximum air detrainment reached 11.3% downstream of the second aerator.
The Quality of Master of Translation and Interpreting Education Abroad: History, Problems and Implications
Authors: Shi Jiaying , Lv Hongyan 
Published: July 2024

After more than a decade of development, the scale of Master of Translation and Interpreting (MTI) education has continuously expanded, yet its teaching and management have also revealed several issues that have affected the quality of cultivating translation talents. This article outlines the development history of MTI education abroad, pointing out the dilemmas and problems it faces, such as lagging curriculum settings, inadequate faculty resources, and insufficient practical ability training. To address these issues, the article puts forward several key insights: first, MTI education should keep up with industry development; second, it should focus on enhancing practical abilities and strengthening professional awareness of students; third, it is necessary to strengthen the construction of faculty resources and improve teaching quality. These insights serve as significant references for improving the quality of MTI education in China.
BENTUK, MAKNA LOGIS, DAN PERAN KONJUNGSI DALAM VIDEO TANGGAPAN ANIES BASWEDAN TERHADAP HASIL PILPRES INDONESIA 2024 DI YOUTUBE
Authors: Darmawan Adhitya , Rohmah Nada Humaida , Sumarlam  
Published: July 2024

This research focuses on revealing the form, logical meaning, and role of conjunctions from the discourse of presidential candidate Anies Bawedan towards the results of the 2024 Indonesian presidential election. The focus of this research is done by using a discourse analysis approach through the lens of Systemic Functional Linguistics, especially on conjunctions. The conjunction theory used belongs to Martin and Rose in Wiratno. The paradigm of this research is qualitative research because the nature of reality of this research is plural and constructed holistically based on its context. The data in this study were collected using purposive sampling technique. The method used in this research is the listening method (accessing and watching a video on YouTube entitled “Pesan Anies Baswedan Usai Kalah Quick Count, Minta Pendukung Relawan Lawan Kecurangan" uploaded on the MerdekaDotCom YouTube channel), with a follow-up technique, namely the note-taking technique by transcribing Anies Baswedan's oral speech in the video. The data analysis procedure of this research uses the discourse analysis model through description, interpretation, and ends with explanation. The results of this study show that 1) the external conjunction of addition is more often used by Anies Baswedan to expand events (ideology), 2) the internal conjunction that Anies often uses is the internal conjunction of inferential consequence to conclude the logic built previously.
Keywords: conjunction, politician, Systemic Functional Linguistics, Discourse
ANALISIS PERILAKU KONSUMEN DIEVHA CAFÉ DI KECAMATAN SUMBER JAYA KABUPATEN LAMPUNG BARAT
Authors: Muslimah Putri Ayu , Indriani Yaktiworo , Adawiyah Rabiatul 
Published: July 2024

This research aims to analyze decision making, attitude, satisfaction and customer loyalty of Dievha Café in Sumber Jaya District, West Lampung Regency. The method used in this research is a survey method by conducting direct interviews using questionnaires to 60 respondents who have made at least 2 purchases during the last 3 months and are at least 17 years old. Data collection was carried out in January-February 2023. The Data are analyzed descriptively using Fishbein's multiattributes, Customer Satisfaction Index (CSI), and loyalty pyramid. The results of this research showed that the first reason consumers buying were because they wanted to try the products, they got the location information from their friends and their main consideration for purchases was the taste. The consumers would switch to other menus when the product was run out and they decided to buy themselves on purpose. All of the consumers were interested in repurchases and 63.33 percent of them were satisfied. From the analysis of Fishbein’s multiattributes showed the score was 136.95 or in a good category. The most preferred attribute was taste with score of 18.13, followed by the location attribute and the employee service attribute. The level of consumer satisfaction on Dievha Café products obtained a value of 82.77 percent or categorized as very satisfied with the highest score attribute was the taste. The level of consumer loyalty to Dievha Café based on the loyalty pyramid analysis was in the satisfied buyer category (73.33%), followed by liking the brand (71.67%), committed buyer (70.00%), switcher buyer (40.00%), and habitual buyer (36.67%).Keywords: coffee shop, consumer attitudes, decisions making, loyalty, satisfaction.
VALUASI EKONOMI DENGAN METODE TRAVEL COST DAN KEPUASAN PENGUNJUNG OBYEK WISATA GRAND ELTY KALIANDA RESORT KABUPATEN LAMPUNG SELATAN
Authors: Reynaldo Wahyu Gusri , Murniati, M.T.A. Dr. Ir. Ktut , Firdasari Firdasari 
Published: July 2024

Penelitian ini bertujuan untuk menganalisis faktor-faktor yang mempengaruhi frekuensi kunjungan wisatawan ke Grand Elty Kalianda Resort, nilai ekonomi Grand Elty Kalianda Resort, dan kepuasan pengunjung terkait atribut 4A atraksi, aktivitas, aksesibilitas, dan fasilitas. Lokasi penelitian dipilih secara purposif dengan pertimbangan bahwa Grand Elty Kalianda Resort merupakan salah satu tempat wisata terbaik di Kabupaten Lampung Selatan. Metode yang digunakan dalam penelitian ini adalah survei yang melibatkan 76 responden. Pengumpulan data dilakukan dengan wawancara langsung pada bulan November 2022 hingga Desember 2022. Metode analisis data yang digunakan adalah analisis regresi linier berganda, metode biaya perjalanan (TCM), analisis Indeks Kepuasan Pelanggan (CSI), dan Analisis Importance Performance (IPA). Hasil penelitian menunjukkan bahwa berbagai faktor yang mempengaruhi jumlah kunjungan ke Grand Elty Kalianda Resort adalah pendapatan, biaya perjalanan, lama perjalanan, sarana dan prasarana, dan kepuasan pengunjung. Nilai ekonomi objek wisata Grand Elty Kalianda Resort sebesar Rp12.501.262,00 per tahun. Sedangkan tingkat kepuasan pengunjung terkait atribut 4A sebesar 91,60 persen menunjukkan pengunjung sangat puas. Pengukuran kepuasan pengunjung dengan metode IPA diperoleh hasil kepuasan pada atribut pantai, rekreasi dan fotografi. Kata kunci: Penilaian ekonomi, metode biaya perjalanan, kepuasan pengunjung.
Nilai-Nilai Pendidikan Dalam Dongeng Janji Seekor Tikus dan Semut Terbitan FAM Publishing Edisi Februari 2018
Authors: Subrata Doni 
Published: July 2024

Penelitian ini merupakan penelitian kualitatif deskriptif yang bertujuan untuk mendeskripsikan dan menganalisis nilai-nilai pendidikan dalam kumpulan cerita dongeng Janji Seekor Tikus dan Semut terbitan FAM publishing edisi Februari 2018. Nilai-nilai pendidikan dalam suatu cerita mencakup sejumlah aspek yang dapat memberikan kontribusi signifikan pada pengembangan pengetahuan, keterampilan, dan pemahaman moral. Diantara nilai-nilai tersebut terdapat nilai pendidikan moral, nilai pendidikan sosial, dan nilai pendidikan religi dimana nilai ini menyampaikan pesan moral dan etika melalui karakter, konflik, dan perkembangan plot yang ada di dalam suatu karya sastra melalui peristiwa yang dihadapi oleh tokoh-tokoh dongeng. Dalam Penelitian ini ditemukan beragam nilai-nilai pendidikan yang terdapat di dalam kumpulan dongeng yang berjudul Janji Seekor Tikus dan Semut. Dalam dongeng ini ditemukan nilai amanah, tepat janji, penyayang, sabar, peduli, suka menolong, sikap memaafkan, kepedulian, sikap berbagi, kepercayaan terhadap takdir Allah, selalu membiasakan berdoa, dan bersedekah kepada orang lain.
Informality and Inclusion: Assessing the effects of the Shadow Economy and informal labour in Europe
Authors: Davidescu Adriana AnaMaria , Manta Eduard Mihai , Geambasu Cristina Maria , Birlan Ioana 
Published: July 2024

Abstract
The informal economy, often characterized by unregulated and untaxed economic activities, has significant yet understudied implications for sustainable development and inclusivity. This paper explores the influence of the size of the shadow economy and undeclared work patterns (envelope wage, without a formal contract and bogus self-employed) on the Leave No One Behind Index and the Sustainable Development Index developed by the United Nations across European countries from 2000 to 2021. Employing panel data regression models, the research investigates the differential impacts of informality on these indices, revealing a negative correlation with the informal economy and a positive association with various forms of undeclared work. The analysis, comprising 18 models segmented by region – Central and Eastern Europe, Western Europe, and Europe – demonstrates that the adverse effects of the informal economy are more pronounced, whereas the positive contributions of undeclared work patterns are subtler, yet significant. The study’s findings suggest a complex interaction between informal economic activities and sustainable development goals, challenging the conventional perspective on informality and its role in inclusive development. The results indicate that while the shadow economy may hinder broad development efforts, informal labor arrangements support vulnerable populations, underscoring the need for nuanced policy interventions that recognize the multifaceted nature of work and its impacts on development.