bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of High Intensity Interval Exercise on Platelet Engraftment in Autologous Bone Marrow Transplantation (BMT)
Authors: Zarekar Tayebe , Hajifathali Abbas , Ahmadizad Sajad 
Published: July 2024

Background: Thrombocytopenia is a frequent complication after hematopoietic stem cell transplantation (HSCT). Although platelet transfusion is the most used treatment for severe thrombocytopenia, it is associated with well-established risks. High-intensity interval exercise (HIIE) results in thrombocytosis. Therefore, this study aimed to reduce thrombocytopenia by increasing platelet count through exercise.
Materials and Methods: Twenty lymphoma and multiple myeloma patients were divided into HIIE and control groups. To determine the maximal exercise capacity, patients in the HIIE group performed a graded exercise test. All patients received granulocyte colony-stimulating factor for 5 days, followed by a HIIE trial. After 5 min warm up at 10 to 20% of peak power, patients in the HIIE group performed an HIIE protocol that included 12 intervals of one-minute work at 100% peak power interspersed by one-minute active rest at 20% of peak power. Patients in the control group were seated for the same duration without any physical activity. Two blood samples were taken before and immediately after the trials and were analyzed for measuring complete blood count.
Results: Platelet count on the day of platelet engraftment in the HIIE group was significantly higher than in the control group (P=0.02). Single-donor platelet transfusion was significantly lower in the HIIE group than in the control group (P=0.05).
Conclusion: Based on the findings of the present study, a short bout of HIIE had a positive effect on platelet engraftment through thrombocytosis and reduced platelet transfusion and its complications, which could be a useful strategy for HSCT patients.
Multi-representation Analysis of General Chemistry Books on Chemical Bonding Subject
Authors: Rizal  , Wiji  , Tuszie Widhiyanti  , Novianti Islahiah  
Published: July 2024

This research is a preliminary study that focuses on multi-representation analysis of the chemical bonding topic in three general chemistry of text books. The analysis was carried out to find out how concepts are explained based on the three levels of chemical representation, especially on ionic and covalent bonding, which are the basis for further research in developing intertextual-based learning strategies. Analysis was carried out at the macroscopic-symbolic and sub microscopic-symbolic levels usingmulti-representation analysis table that was adopted from Gkitzia et al., 201 for three general chemistry books, that are 1) Whitten, K.W, et.al. (2014). Chemistry 10th edition. English: Brooks/Cole Cengage Learning; 2) Chang, R. (2010). Chemistry 10th edition. New York : The McGraw-Hill Companies; and 3) Silberberg, M.S, and Amateis, P.G. (2021). Chemistry: the molecular nature of matter and change 9th edition. New York : The McGraw-Hill Companies. The analysis results show that book: Silberberg, M.S, and Amateis, P.G. (2021). Chemistry: the molecular nature of matter and change 9th edition. New York : The McGraw-Hill Companies has the most complete macroscopic-symbolic and sub microscopic-symbolic representation levels on the four main concept labels of chemical bonding, so that they can become standard reference material in developing chemical representations that will be used in intertextual learning strategies.
Prediction Prediction of the Insecticidal Potency of Biduri Plants (Calotropis gigantea) using the PASS online web resource
Authors: Sururin Fahma Wardah , Khafiyya Nida An 
Published: July 2024

Calotropis gigantea, a wild plant thriving in arid environments, has been traditionally used for medicinal purposes by communities near Baluran National Park (BNP). The latex of C. gigantea is used as a crab poison due to its ability to cause fatal damage and separation of body parts, possibly related to chitin disruption in insects. This study explores the potential insecticidal properties of secondary metabolites in C. gigantea using Prediction of Activity Spectra for Substance (PASS) Online. Out of 68 identified secondary metabolites, six show significant insecticidal potential, namely Profenophos, Ethion, Alpha-Citral, 1-Phenylethyl acetate, (E)-dec-3-en-2-one, and Benzaldehyde. Notably, Profenophos, Ethion, Alpha-Citral, and Benzaldehyde exhibit toxic properties effective against insects, with enzyme inhibitory activity affecting nerve signaling and immune systems, suggesting potential for bio-insecticide development.
“Mais que uma ideia na cabeça”
Authors: Fragoso Mariana Pitasse 
Published: July 2024

Este artigo tem como objetivo refletir sobre como se organizam sentidos, valores e relações em torno do boné do Movimento dos Trabalhadores Rurais Sem Terra (MST). O adereço vermelho – que tem encontrado adeptos fora do espaço da militância –, pode ser entendido muito além de um acessório que demarca uma posição política ou a integração ao movimento. Na relação entre lideranças e militantes, o boné pode ser utilizado como ferramenta que aponta as posições que ocupam na estrutura do movimento. As análises contidas neste artigo são construídas em torno da descrição de um episódio, que envolve a transferência de uma posição de liderança de um militante para outro, através de uma “passagem de boné” – que neste caso não se trata só de uma figura de linguagem para simbolizar a mudança, mas também do adereço propriamente dito cedido como herança. O episódio exemplifica a mobilização de um conjunto de categorias nativas principalmente expressas pela “mística”, pelo “compromisso”, pela “luta” e pela “confiança”. As considerações apresentadas ao longo texto foram estruturadas a partir de pesquisa empírica realizada por meio de observação participante e entrevistas com militantes e lideranças do MST.
Audiovisual Translation as a Media for Teaching EFL Vocabulary to Primary School Students in Indonesia
Authors: Kembaren Farida Repelita Waty , Rajagukguk Sahmiral Amri , Pratiwi Anggun Siska , Khairani Khairani 
Published: July 2024

This research demonstrates the important role of technology in education by highlighting the potential of audiovisual techniques as learning media for primary school students. Previous research studies have shown that appropriate audiovisual presentations can improve comprehension, especially in today's children who are enthusiastic about multimedia technology. A qualitative research method using a classroom action research design was used to carry out the research procedure, while thematic analysis was used to analyse the data and evaluate the findings. The aim of this study is to evaluate one of the most effective EFL vocabulary teaching methods for primary school students, namely audiovisual translation, and to summarise the students' impressions while learning vocabulary using audiovisual translation. This research contributes to innovative EFL teaching methods by suggesting the widespread use of audiovisual translation in primary schools around the world, especially in Indonesia, based on its proven success and positive responses from students.
First modern morphological and molecular description of Saccocoelium cephali larvae stages (Digenea: Haploporidae) from the Black Sea
Authors: Belousova Yuliya , Atopkin Dmitry M. 
Published: July 2024

Abstract

Purpose
Knowledge of haploporid trematode larvae is very poor. Until recently, only scattered reports from Black and Medditeranean Seas are known. The present research is the first report of haploporid cercariae S. cephali from the gastropod Hydrobia acuta inhabiting the Black Sea. Thus, the present study aimed to investigate the larvae of one species from Haploporidae family.
Methods
Hydrobia acuta molluscs were collected in the estuary of the Chernaya River ( Black Sea) during 2011-2013 and examined for trematode larvae. Found parthenitae were drawn and preserved for DNA extractions. Morphological features were compared to nominal species and genetic comparisons were made of the subunit ribosomal DNA with other Haploporidae species.
Results
The haploporid parthenitae were found in the gonads of Hydrobia acuta molluscs. Most of morphological characteristics of specimens investigated agree with those of Saccococoelium sp. and closest to S. cephali by shape and length of the body, ratio of suckers, ratio of tail length to body, shape and size of pharynx. Phylogenetic analysis from our study generated with Bayesian algorithm showed that studied cercariae specimens ex H. acuta from the Black Sea was within hapolporine clade and closely related with S. cephali from Spain. The process of encystation of 1 cercaria in same mollusc was observed (adolescaria).Cercariae and metacercariae have been described and named as Saccocoelium cephalibased on its morphological, morphometric and molecular characteristics.
Conclusion
Morphological characteristics of cercariae emerging from Hydrobia acuta from Black Sea, also the analysis of partial 28S rDNA sequences support conspecificity of parasite with S.cephali mature worms from Mediterranean Sea.
A missense mutation in PDHB gene: identification of the patient with Pyruvate dehydrogenase deficiency and demonstration of pathogenicity in vitro
Authors: Li Ying , Cheng Lin , Li Xin , Liu Jiyu , Yi Lu , Bo Tao 
Published: July 2024

Abstract

Background: Pyruvate dehydrogenase (PDH) deficiency is a common cause of primary refractory lactic acidosis. PDH E1β (PDHB) subunit gene mutation is rare causes of PDH deficiency. We described a missense mutation of PDHB gene in a neonate with PDH deficiency, and verify the mutation damages PDH activity in vitro.
Methods: Whole exome sequencing (WES) was used to discover the missense mutation. We constructed the recombinant eukaryotic recombinant expression vector the phage-PDHB-wt/mut containing human full-length wild-type (NM_000925.4) or mutant (c.575G>T) PDHB gene, and transfected vector into 293T cells. Western blot was performed to assess PDH protein stability, PDH activity was measured.
Results: A 37-week-gestation male infant was noted to have refractory lactic acidosis, growth retardation, and neurodevelopmental abnormalities with abnormal cranial MR findings, starting with convulsive seizures at 3 months of age. WES analysis revealed the homozygous missense mutations in the PDHB gene, which was c.575G＞T (p.Arg192Leu) in exon 6. This missense mutation of PDHB was predicted to be harmful by bioinformatics software including Sorting Intolerant From Tolerant (SIFT), Polyphen2, LRT, and Mutation Taster. Western blot showed that normal PDH protein expression was significantly decreased in the phage -PDHB-mut transfected cells than that in the phage -PDHB-wt transfected cells (P<0.001) . PDH activities analysis revealed that PDH activity was significantly decreased in the phage -PDHB-mut transfected cells than that in the phage -PDHB-wt transfected cells (P<0.001).
Conclusions: c.575G＞T (p.Arg192Leu) in PDHB gene is a pathogenic missense mutation, which causes PDH deficiency in autosomal recessive inheritance mode.
Unveiling Fungal Proficiency in Microplastic Degradation: A Comprehensive Research Investigation
Authors: ARIF SHAYMAA , Hassan Fikrat M. , Fakhry Saad Sabah , Al-Qaysi Safaa Al-Deen Ahmed Shanter , Adnan Safauldeen 
Published: July 2024

Abstract

Microplastics (MPs) are prevalent pollutants in environments that are colonized by various groups of microbes. Fungi are considered among the most efficient microbial degraders of MPs because they produce salient enzymes and can survive on recalcitrant compounds with limited nutrients. While most studies have focused on the occurrence of MPs in wastewater treatment systems, MP degradation in fresh water and wastewater is generally poorly understood. Therefore, the current study included the isolation of some genera of fungi from the Tigris River water environment that have the ability to degrade MPs in both natural and artificial environments utilizing synthetic media. Using weight loss measurements, Fourier transform infrared spectroscopy (FTIR) was used to identify the chemical structure of the plastic polymers, and scanning electron microscopy (SEM) was used to determine the size and morphology of the microplastics and the degree of plastic consumed by the aquatic fungus. The biodegradation of high-density polyethylene (HDPE) and polystyrene (PS) by the aquatic fungus Aspergillus carbonarius and Eurotium sp. was also examined. Overall, Aspergillus carbonarius and Eurotium sp. were able to degrade HDPE more efficiently than PS without requiring any prior microplastic treatment. Therefore, the ability of fungi to degrade MPs was confirmed by weight loss, FTIR, and SEM data. Therefore, the results indicate that the isolated fungus has a promising future for polymer breakdown in both artificial and natural environments. Investigating the long-term impacts and gaining a deeper knowledge of the mechanisms of microplastic disintegration should be the main goals of future research.
Single-cell transcriptomics reveal the crosstalk between platelet-associated TRAF4high endothelial cell subpopulation and tumor microenvironment in colorectal cancer
Authors: Su Guoqing , Wang Nannan , Cao Yuepeng , Qian Jinghang , Wang Yi , Si Chengshuai , Shao Peng , Wang Qifan , Ye Yongxia , Wang Ke , Bao Jun , Yang Liu 
Published: July 2024

Abstract

Background
Platelet-tumor endothelial cell interactions are pivotal in the colorectal cancer microenvironment, driving tumor invasion, metastasis and shaping the immune landscape. Investigating these interactions sheds light on the underlying mechanisms of colorectal cancer progression and opens avenues for novel therapeutic interventions.
Methods
By interrogating 10x Genomics single-cell transcriptomic data from 23 publicly available samples, we constructed a paired cancerous and adjacent non-cancerous single-cell atlas for colorectal cancer (CRC). Utilizing a comprehensive suite of analyses, including area under the curve (AUC), copy number variation, pseudotime trajectory, enrichment, and intercellular communication assessments, we delineated the heterogeneity within CRC endothelial cells. Furthermore, our SCENIC transcription factor analysis pinpointed the most active transcription factors within the platelet-associated endothelial cell subpopulation. The functional relevance of TRAF4 in endothelial cell was subsequently confirmed by in vitro validation and large cohort clinical samples.
Results
We delineated a distinct subpopulation of endothelial cells, designated as C2, that is profoundly influenced by platelet education and is intricately linked to tumor metastasis, adverse prognosis, and immune cell infiltration. This subpopulation exhibits a strong association with the immune cell infiltration characteristic of the tumor microenvironment. Notably, TRAF4 emerged as a pivotal platelet-related transcription factors within this subgroup, with its downregulation leading to diminished migration, angiogenic potential, and chemotactic responsiveness of endothelial cells to CD8 + T cells. Additionally, the expression of TRAF4 and its correlation with CD8 + T cell infiltration were corroborated in clinical specimens.
Conclusions
Our investigation has identified a platelet-associated subpopulation of tumor endothelial cells that contributes to the suppression of CD8 + T cell infiltration within the tumor microenvironment and promotes tumor angiogenesis, thereby facilitating malignant tumor progression. Furthermore, we have pinpointed TRAF4 as a pivotal transcription factor within this subpopulation, underscoring its significant promise as an innovative therapeutic target for CRC.
Assessing Community Awareness and Engagement in the Prevention and Control of Non-Communicable Diseases (NCDs) in East Wollega Zone, Nekemte town: A Comprehensive Study
Authors: Amenu Desalegn , Nugusa Ayantu , Tafesse Temesgen 
Published: July 2024

Abstract

Background: Non-communicable diseases (NCDs) pose a significant health challenge globally, including in East Wollega Zone, Ethiopia, particularly in Nekemte town. The burden of NCDs is rising, necessitating a thorough assessment of community awareness and engagement for effective prevention and control strategies. This study aims to assess the level of community awareness and engagement in NCD prevention and control in Nekemte town, East Wollega Zone,, with more emphasis evaluating awareness levels, health behaviors, existing programs, barriers to engagement, and best practices. A mixed-methods approach was employed, including surveys, interviews, focus group discussions, and review of existing data and literature. Demographic data, prevalence rates, awareness levels, health behaviors, and community perceptions were collected and analyzed. The study will provide insights into the current state of community awareness and engagement regarding NCDs in Nekemte town. Findings were including awareness gaps, prevalent health behaviors, barriers to engagement, and successful community programs. For further intervention, evidence-based interventions and policies aimed at improving community awareness, promoting healthy behaviors, reducing NCD burden, and enhancing community engagement in Nekemte town and similar settings. The study is very important and has the potential to improve health outcomes, optimize resource allocation, reduce disparities, empower communities, inform policies, contribute to academic knowledge, and impact global health initiatives related to NCD prevention and control.
Early warning study of Malan loess collapse damage based on acoustic emission technology
Authors: Zhang Ke , Li Genqiang , Wang Liang 
Published: July 2024

Abstract

Loess collapse is one of the most developed geohazards in loess area, and the acoustic emission active waveguide model is an important tool for monitoring and early warning of loess collapse geohazards and acquisition of precursor information. In this study, the acoustic emission active waveguide model is used as the research object, and by carrying out the acoustic emission active waveguide model pressurization test and wavelet noise reduction processing of the acquired acoustic emission data, the evolution of acoustic emission signals in the process of acoustic emission pressurization of primary Malan loess is revealed in various phases, including compaction, elasticity-plasticity deformation, destruction, and post-destruction phases. The attenuation characteristics of the AE signal propagation paths of the structures comprising the active waveguide model, including geometric diffusion attenuation, material absorption attenuation, and coupling attenuation, are described. The study also showed that the integrated damage form was identified based on the acoustic emission RA-AF value characteristics, and 63% of the high RA value and 37% of the high AF value, which is "high RA value and low AF value", indicating that the integrated damage form of the in-situ loess collapse damage under this monitoring condition is shear damage. At the same time, the acoustic emission b-value characteristics of the collapse damage of the primary Malan loess were investigated, with a rapid increase and a small amount of acoustic signals at the early stage of pressurization, a rapid decrease and more acoustic signals at the middle stage of pressurization, and a continuous decrease and a large amount of acoustic signals at the middle and late stages of pressurization. The early warning precursor information obtained from in situ loess acoustic emission monitoring provided in this paper can be used in the construction of monitoring and early warning system for geologic disasters such as loess collapse in loess areas.
Exercise training increase BDNF/TrkB mRNA, prevents memory loss and ameliorate the hippocampal morphology in multiple sclerosis model
Authors: Hafedh Muthanna , Hatem Muthana Layth 
Published: July 2024

Abstract

Purpose: We aimed to verify the implications of four weeks of consistent exercise on the mRNA of the brain-derived (BDNF) and the tropomyosin-related kinase B (TrkB) receptor that may contribute to ameliorates the hippocampal morphology and induced enhancement of memory in experimental autoimmune encephalomyelitis (EAE) after the diseases recovery stage.
Methods: Female Lewis rats were randomly separated into three groups, control group (Co), EAE without exercise (EAE), EAE with exercise (EAE+Ex) , each group contains (10 rats) . All rats underwent to the Morris water maze (MWM) test for four days in week and prop test was done in fifth day and repeated in day 30 post induction. Exercise was prescribed by motorized treadmill starting at 17 m/min and gradually increased, a total duration of 30 minutes per day, five days a week and discontinued two weeks after the induction. EAE was induced subcutaneously after two weeks of exercise as rats injected with an inoculum containing 50 ug guinea pig spinal cord homogenate emulsified in incomplete Freund’s adjuvant and 2mg of heat-mycobacterium at the tail base.
Results: EAE+Ex significantly increases the BDNF mRNA (P< 0.05) and TrkB (P> 0.02) mRNA levels compared to the EAE groups. The Co group increased significantly more than the EAE group (P< 0.01). Swimming speed performance was faster in EAE+Ex (P< 0.02) compared to EAE group lead to improved hippocampus function.
Conclusion: It seems that the provision of four weeks of exercise training is capable of increasing the mRNA of BDNF and its receptor along with ameliorating the hippocampus morphological which can have positive effects on memory impairment during EAE disease.
Activities of Soil C and N Enzymes during Charcoal Production in Derived Savannah of Nigeria
Authors: Adeyemo Adebayo Jonathan , Adejoro Solomon Alaba , Adegbile Oluwagbemi Israel , Babalola Temitope Seun , Akinnagbe Evelyn Atinuke , Awodun Moses Adeyeye , Oyun Matthew Banji 
Published: July 2024

Abstract

In recent years, anthropogenic activities, particularly charcoal production and the utilization of large fuelwoods have significantly compromised the deforestation of savannah forest soils in southwest Nigeria. This has had a notable impact on soil enzymes and nutrient cycles. Despite the growing acknowledgment of charcoal’s significant impact on soil properties, there is a notable gap in the literature regarding its specific effects on soil carbon (C) and nitrogen (N) enzymes. To address this, our study therefore aims to examine the effects of charcoal production on the C and N enzymes activities, under different locations and soil depths in the derived savannah of southwestern Nigeria. Soil samples were systematically collected and analyzed at depths 0–15, 15–30, and 30–45 cm under different locations of charcoal production and non-production sites, CPS and NPS respectively. The result showed a significantly higher activity level for amylase (0.54 µg/ml/min) and cellulase (0.90 µg /ml/min) at the NPS compared with CPS. In contrast, the protease and urease activity tended to be higher at CPS (0.54 and 16.66 mg/ml/min respectively) than at NPS, suggesting distinct protein degradation patterns at both sites. In terms of enzyme ratios, amylase/cellulase activity levels is greater at CPS than NPS with no significant difference with values of 1.44 and 0.79 µg/ml/min respectively. Similarly, protease/urease activity ratio levels are identical at both sites (0.04 mg/ml/min). Yet, this ratio decreases with depth due to heightened protease and urease activities, catalyzing the mineralization of organic nitrogen, and resulting in increased nitrogen content and the release of ammonia-N in soils. Overall, our findings suggest that the charcoal production process may influence and alter soil enzyme activities, potentially impacting the breakdown of carbohydrates, cellulose, proteins, and urea in the soil. These variations in enzyme activities could have broader implications for nutrient cycling, microbial processes, and overall soil health in areas with charcoal production areas compared to non-production sites.