bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proportion cured models applied to 23 cancer sites in Norway
Authors: Cvancarova Milada , Aagnes Bjarte , Fosså Sophie D. , Lambert Paul C , Møller Bjørn , Bray Freddie 
Published: August 2012

AbstractStatistical cure is reached when a group of patients has the same mortality as cancer‐free individuals. Cure models predict the cured proportion and the median survival of fatal cases. Cure models have seldom been applied and tested systematically across all major cancer sites. Incidence and follow‐up data on 23 cancer sites recorded at the Cancer Registry of Norway 1963–2007 were obtained. Mixture cure models were fitted to obtain trends and up‐to‐date estimates (based on period approach) assuming cured and uncured groups exist. The model converged for cancers of the mouth and pharynx, oesophagus, stomach, colon, rectum, liver, gallbladder, pancreas, lung and trachea, ovary, kidney, bladder, CNS, non‐Hodgkin lymphoma (only for males) and leukemia. The proportion of cured patients increased 1963–2002 for both sexes, with the largest changes (in percent) seen for leukemia (46.4 and 46.7) and CNS (35.9, 42.0), males given first. Median survival time for the uncured cases increased for colon and rectal cancer, and there was a three‐ fold increase in median survival time for patients with fatal ovarian cancers. Cancers of bladder and CNS had the highest up‐to‐date proportion cured (in percent), 67.4 and 64.0, respectively, pancreas and liver were amongst the lowest (5.7 and 9.9, respectively). Cure models are useful when monitoring progress in cancer care, but must be applied and interpreted with caution. The absolute estimates of the cure proportion are speculative and should not be calculated where cure is not medically anticipated.
THE EFFECT OF BOUNDARY CONDITIONS ON EFFICIENCY AND PRICING IN DOUBLE‐AUCTION MARKETS WITH ZERO‐INTELLIGENCE AGENTS
Authors: Miller Ross M. 
Published: August 2012

SUMMARYThe computational tractability of many markets with computerized agents requires they be subject to boundary conditions that are both unnecessary and undesirable in markets with humans. In particular, double‐auction markets with zero‐intelligence agents, which are a standard baseline in computational economics, constrain all bids, offers and trade prices to lie between an upper and a lower bound. This paper shows how changes in these boundary conditions influence pricing and efficiency. It is found that boundary conditions consistent with competitive pricing are generally inconsistent with the most efficient operation of those markets. Copyright © 2012 John Wiley & Sons, Ltd.
Immobilization of cell‐binding peptides on poly‐ε‐caprolactone film surface to biomimic the peripheral nervous system
Authors: de Luca A. C. , Stevens J. S. , Schroeder S. L. M. , Guilbaud J.‐B. , Saiani A. , Downes S. , Terenghi G. 
Published: August 2012

AbstractCell‐material interactions are crucial for cell adhesion and proliferation on biomaterial surfaces. Immobilization of biomolecules leads to the formation of biomimetic substrates, improving cell response. We introduced RGD (Arg‐Gly‐Asp) sequences on poly‐ε‐caprolactone (PCL) film surfaces using thiol chemistry to enhance Schwann cell (SC) response. XPS elemental analysis indicated an estimate of 2–3% peptide functionalization on the PCL surface, comparable with carbodiimide chemistry. Contact angle was not remarkably reduced; hence, cell response was only affected by chemical cues on the film surface. Adhesion and proliferation of Schwann cells were enhanced after PCL modification. Particularly, RGD immobilization increased cell attachment up to 40% after 6 h of culture. It was demonstrated that SC morphology changed from round to very elongated shape when surface modification was carried out, with an increase in the length of cellular processes up to 50% after 5 days of culture. Finally RGD immobilization triggered the formation of focal adhesion related to higher cell spreading. In summary, this study provides a method for immobilization of biomolecules on PCL films to be used in peripheral nerve repair, as demonstrated by the enhanced response of Schwann cells. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Different hyaluronic acid morphology modulates primary articular chondrocyte behavior in hyaluronic acid‐coated polycaprolactone scaffolds
Authors: Lebourg Myriam , Rochina Joaquin Rodenas , Sousa Tiago , Mano João , Ribelles José Luis Gómez 
Published: August 2012

AbstractScaffolds for cartilage tissue engineering should promote both adequate biomechanical environment and chondrogenic stimulation. Hyaluronic acid (HA) has been used in cartilage engineering for its chondrogenic and chondroprotective properties, nevertheless its mechanical properties are limited. Influence of HA microstructure in chondrocyte response has not been addressed yet. In this work, polycaprolactone (PCL) scaffolds were modified using HA following two coating strategies: coating in one step (PCL‐HA1s) yields a gel‐like phase within the scaffold, whereas a two‐step reaction (PCL‐HA2s) yields a thin HA layer coating internal surfaces of PCL structure. Chondrocytes were seeded in the scaffolds and cultured in dedifferentiating conditions up to 3 weeks and analyzed using a total DNA assay and sulfated glycosaminoglycan (sGAG) determination assay; cell morphology and extracellular matrix secretion were assessed by electron microscopy as well as immunofluorescent imaging (collagen I, collagen II, aggrecan, CD44). Cells proliferate in all samples and no cytotoxicity is observed. PCL‐HA1s shows higher sGAG production per cell than PCL and PCL‐HA2s at all times. Presence of hyaluronic acid promotes qualitative expression of CD44 surface markers and aggrecan (more visible in PCL‐HA1s than PCL‐HA2s), whereas in dedifferentiating conditions, expression of CD44 and aggrecan can hardly be detected in pure PCL scaffolds. Collagen type II seems more prominent in PCL‐HA2s; although PCL‐HA2s shows markers for COL II, aggrecan and CD44, quantitative ECM production is not improved with respect to PCL. It is thus likely that CD44 activation is not sufficient for explaining the better response in PCL‐HA1s. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Cellular interactions and stimulated biological functions mediated by nanostructured carbon for tissue reconstruction and tracheal tubes and sutures
Authors: Misra R. D. K. , Chaudhari P. M. 
Published: August 2012

AbstractNylon 6,6 is used for biological applications including gastrointestinal segments, tracheal tubes and sutures, vascular graft, and for hard tissue reconstruction. While it is a relatively inexpensive polymer, it is not widely acceptable as a preferred biomaterial because of bioactivity. To this end, we have discovered the exciting evidence that introduction of a novel nanostructured carbon, graphene, in the void space between the nylon chains and processing at elevated pressure favorably stimulates cellular functions and provides high degree of cytocompatibility. The cell–substrate interactions on stand alone Nylon 6,6 and Nylon 6,6‐graphene oxide hybrid system were investigated in terms of cell attachment, viability, proliferation, and assessment of proteins, actin, vinculin, and fibronectin. The enhanced biological functions in the nanostructured hybrid system are attributed to relatively superior hydrophilicity of the surface and to the presence of graphene. Furthermore, it is proposed that the negatively charged graphene interacts with the polar nature of cells and the culture medium, such that the interaction is promoted through polar forces. This is accomplished by investigating cell attachment, proliferation, and morphology, including cytomorphometry evaluation, and quantitative assessment of prominent proteins, actin, vinculin, and fibronectin that are sensitive to cell–substrate interactions. Osteoblasts were studied to establish the practical viability of the hybrid nanostructured biomaterial. The study strengthens the foundation for utilizing nano‐ or quantum‐size effects of nanostructured biomaterials. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
High yield isolation of BMP‐2 from bone and in vivo activity of a combination of BMP‐2/TGF‐β1
Authors: Sibiya Sibusiso J. , Olivier Eugene I. , Duneas Nicolaas 
Published: August 2012

AbstractA high‐yield purification procedure for protein fractions derived from porcine bone matrix extracts is described, which has a high abundance of bone morphogenetic protein‐2 (BMP‐2). Naturally derived pBMP‐2, ∼5 μg per kilogram of porcine bone matrix, was isolated by using a 300 kDa membrane before chromatographic processing on heparin affinity media. The elution of pBMP‐2 and transforming growth factor‐β1 (TGF‐β1) revealed morphogen peaks that were unresolved on Prosep® medium, but resolved on hydroxyapatite medium. Antagonism was observed in animal studies when the two proteins were combined in specific doses. The TGF‐β1 fraction alone was not active in the rodent heterotopic in vivo bioassay, confirming previously obtained results. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 641–646, 2013.
Human Wharton's Jelly stem cell conditioned medium and cell‐free lysate inhibit human osteosarcoma and mammary carcinoma cell growth in vitro and in xenograft mice
Authors: Gauthaman Kalamegam , Fong Chui‐Yee , Arularasu Suganya , Subramanian Arjunan , Biswas Arijit , Choolani Mahesh , Bongso Ariff 
Published: August 2012

AbstractHuman Wharton's jelly stem cells (hWJSCs) were shown to inhibit the growth of human mammary carcinomas. It is not known whether cell‐free secretions or lysates of hWJSCs do the same on different cancers. They may be less controversial than cells to regulatory bodies for clinical application. We examined the influence of hWJSC conditioned medium (hWJSC‐CM) and cell‐free lysate (hWJSC‐CL) on two osteosarcoma cell lines (MG‐63, SKES‐1) in vitro and on human mammary carcinomas in immunodeficient mice. When exposed to hWJSC‐CL, increased vacuolations in MG‐63 and increased membrane fragmentation in SKES‐1 cells were observed, with greater cell death in SKES‐1. Exposure of SKES‐1 and MG‐63 cells to hWJSC‐CL showed significant decreases in cell proliferation of 46.48 ± 6.66% and 24.32 ± 5.67% respectively compared to controls. MG‐63 and SKES‐1 cells were annexin V‐FITC positive and SKES‐1 TUNEL positive following treatment with hWJSC‐CM and hWJSC‐CL. MG‐63 cells were positive and SKES‐1 cells negative for anti‐BECLIN‐1 and anti‐LC3B following treatment with hWJSC‐CM and hWJSC‐CL. RT‐PCR showed that the pro‐apoptotic BAX gene and the autophagy‐related ATG‐5 and BECLIN‐1 genes were up‐regulated while the anti‐apoptotic BCL2 and SURVIVIN genes were down‐regulated in MG‐63 and SKES‐1 cells treated with hWJSC‐CM and hWJSC‐CL. Injections of hWJSCs and hWJSC‐CM into mammary carcinomas in immunodeficient mice resulted in decreased tumor sizes and weights of 24.86 ± 6.05% to 37.03 ± 5.91% and 47.14 ± 7.36% to 55.09 ± 5.87% respectively at 6 weeks compared to controls. hWJSC‐CM and hWJSC‐CL inhibit mammary carcinoma and osteosarcoma cells via apoptosis and autophagy. J. Cell. Biochem. 114: 366–377, 2013. © 2012 Wiley Periodicals, Inc.
Glutamate‐ammonia ligase and reduction of G0 population in PANC‐1 cells
Authors: Choi Jong‐Ho , Lim Key‐Hwan , Park Eunmi , Kim Ji‐Young , Choi Young‐Kil , Baek Kwang‐Hyun 
Published: August 2012

AbstractIn our previous study, we screened and isolated genes that were up‐regulated after partial pancreatectomy using transcriptomic analysis and glutamate‐ammonia ligase (GLUL) was selected for further study based on its effect on differentiation and proliferation. In the immunohistochemical analysis, GLUL was highly up‐regulated in the acinar cells and the ductal cells in the pancreas damaged through partial pancreatectomy. Overexpression of GLUL enhanced the proliferation of PANC‐1 cells and INS‐1 cells. GLUL overexpression shifted the major population of PANC‐1 cells from the G0/G1 phase to G2/M phase. In the double thymidine blocking analysis, similar cycle duration was observed between mock cells and GLUL‐overexpressing cells while GLUL‐overexpressing cells were partially resistant to thymidine blocking. In the FACS analysis of cells stained with Pyronin Y and Hoechst 33342, GLUL‐overexpressing cells showed lower population of cells in the G0‐quiescent phase than mock cells (5–12%). In addition, GLUL‐overexpressing cells had high activation levels of AKT, ERK1/2, JNK, PCNA, c‐FOS, and P70S6K in PANC‐1 cells. Taken together, these results suggest that GLUL contributes to pancreatic regeneration. J. Cell. Biochem. 114: 303–313, 2013. © 2012 Wiley Periodicals, Inc.
FOXO3a/p27kip1 expression and essential role after acute spinal cord injury in adult rat
Authors: Zhang Shuangwei , Huan Weipeng , Wei Haixiang , Shi Jinlong , Fan Jinpeng , Zhao Jian , Shen Aiguo , Teng Honglin 
Published: August 2012

AbstractFOXO3a (Forkhead Class box O3a), as an important direct target of the phosphatidylinositol 3‐kinase (PI3K)/protein B (Akt) pathway, which regulates the cell survival and the cell‐cycle progression. Recent reports showed that FOXO3a could inhibit cell‐cycle progression at the G1/S transition by controlling transcription of the cyclin‐dependent kinase inhibitor p27kip1, which is also a key regulator of the mammalian neurogenesis. To elucidate the expression and role of FOXO3a in nervous system lesion and repair, we performed an acute spinal cord contusion injury (SCI) model in adult rats, which showed a temporal–spatial expression pattern of FOXO3a. Temporally, FOXO3a protein level significantly reduced day 3 after injury, and following FOXO3a down‐regulation, p27kip1 protein and mRNA levels were also decreased after injury. Spatially, decreased levels of FOXO3a and p27kip1 were predominant in astrocytes, which were regenerating axons and largely proliferated after injury. Furthermore in vitro, Western blot analysis, RT‐PCR, and immunofluorescence staining analysis demonstrated the relationship between FOXO3a and p27kip1 in primary astrocytes. FOXO3a modulated the cell cycle by transcriptional regulation of p27kip1 in astrocytes. Administration of the PI3K pharmacological inhibitor LY294002 abrogated this effect by regulating FOXO3a and p27kip1 expression and subcellular localization. These results suggest that decreased levels of FOXO3a and p27kip1 in spinal cord are involved in axonal regeneration and the proliferation of glial cells after SCI. J. Cell. Biochem. 114: 354–365, 2013. © 2012 Wiley Periodicals, Inc.
Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1
Authors: Cao Weifeng , Qi Benkun , Zhao Juan , Qiao Changsheng , Su Yi , Wan Yinhua 
Published: August 2012

AbstractBACKGROUND: β‐poly(malic acid) (PMLA) can be used as a pro‐drug or for a drug‐delivery system. Effects of pH, dissolved oxygen concentration (DO) and stirring speed were investigated to improve PMLA production by A. pullulans ipe‐1.RESULTS: The strain produced a high PMLA concentration when pH and DO remained at about 6.0 and above 70%, respectively, and the yeast‐like cells were the main PMLA producers. To further promote PMLA production, the cultivation could be divided into three phases. In phase I, cell growth was accelerated by maintaining high DO (>70%) with a constant stirring speed of 800 rpm. In phase II, PMLA production was increased by controlling DO at 70% using the automatically controlled stirring speed. In phase III, PMLA production on per gram of glucose (Yp/s) was enhanced by keeping DO at 70%, and using a low stirring speed to decrease cell growth. Compared with batch cultures, a higher PMLA yield was obtained with this strategy, i.e. PMLA production and Yp/s increased by 15% and 18%, respectively.CONCLUSION: Control strategies for pH, DO and stirring speed provide a good reference for process development and optimization of PMLA production. © 2012 Society of Chemical Industry
Removal of triclosan by conductive‐diamond electrolysis and sonoelectrolysis
Authors: Martín de Vidales María J. , Sáez Cristina , Cañizares Pablo , Rodrigo Manuel A. 
Published: August 2012

AbstractBACKGROUND: Removal of triclosan by conductive‐diamond electrochemical oxidation (CDEO) and sonoelectrochemical oxidation (CDSEO), either in methanol/water or methanol solutions is studied in the range of concentration 0.1 to 100 mg dm−3. Effects of current density and of electrolyte on the process efficiency are assessed.RESULTS: Concentration of triclosan can be removed below the detection limit of HPLC. In methanol/water solutions, there are two stages: a rapid decrease in the concentration and a less efficient process in which the rate is slower. In methanol solutions, experimental results clearly fit first‐order kinetics. Mediated oxidation plays a significant role. Electrolyses in sulphate media are more efficient than in chloride media and the second low‐rate stage in methanol/water solution appears for higher triclosan removal. The main intermediates are catechol, chlorohydroquinone, 4‐chlorocatechol, acetic acid and dichloroacetic acid. They were only detected during the electrolysis of highly concentrated solution, suggesting a very energetic oxidation.CONCLUSIONS: CDEO and CDSEO are very efficient for the removal of triclosan. CDSEO improves mass‐transfer processes and gives a more efficient removal at lower concentrations. The removal of methanol is improved significantly, in spite of its concentration being well over the limiting value for mass transport control. The reaction sequence for triclosan mineralization is consistent with literature reports. © 2012 Society of Chemical Industry
Beet molasses based exponential feeding strategy for thermostable glucose isomerase production by recombinant Escherichia coli BL21 (DE3)
Authors: Angardi Vahideh , Çalık Pınar 
Published: August 2012

AbstractBACKGROUND: The effects of pretreated beet molasses based feeding strategies on thermostable glucose isomerase (GI) production by recombinant Escherichia coli BL21 (DE3) pLysS were investigated.RESULTS: The thermostable GI encoding gene of Thermus thermophilus (xylA) was recombined with pRSETA vector, and the pRSETA::xylA obtained was transferred into E.coli BL21 (DE3) pLysS and used for GI production. The highest soluble GI activity was obtained at t = 30 h, as A = 16 400 U L−1 (20.6 U mg−1 protein) under molasses based fed‐batch operation, with a specific growth rate µ = 0.1 h−1 (M‐0.1); on the other hand, the highest cell concentration was obtained at µ = 0.15 h−1 operation as 9.6 g L−1 at t = 32 h. The highest oxygen uptake was 4.57 mol m−3 s−1 at M‐0.1 operation.CONCLUSIONS: Molasses based fed‐batch operations were more successful in terms of cell concentration and thermostable enzyme production due to the existence of a natural sugar inducer, galactose, in the molasses composition. This study demonstrates the significance of proper feeding strategy development for over‐production of enzymes by recombinant E. coli strains. © 2012 Society of Chemical Industry
Removal of nutrients from hydroponic greenhouse effluent by alkali precipitation and algae cultivation method
Authors: Saxena Priyanka , Bassi Amarjeet 
Published: August 2012

AbstractBACKGROUND: Hydroponic greenhouse effluent has high concentrations of total phosphorus (30–100 mg PO4‐P L−1) and nitrates (200–300 mg NO3‐N L−1). Current technologies for effluent treatment have limitations of performance and high maintenance costs. The goals of this study were to investigate strategies which combine alkali treatment and microalgae cultivation for removal of nutrients from hydroponic greenhouse effluent.RESULTS: Treatment with strong alkali was found to effectively remove 97% of total phophorous especially in the form of phosphate, without affecting the nitrate ion concentration in the greenhouse effluent. After alkali treatment, marine algae Dunaliella salina (UTEX 1644) cultivation on treated hydroponic effluent (pH 7.5) showed > 80% decrease in nitrate content in the effluent within 4 days of cultivation. In the same period, the carotene content of the micro‐algal system was in the range 0.5 ± 0.02 µg mg−1 (dry cell weight) which was 1.5 times higher than in the control.CONCLUSION: This study demonstrated that combination of a conventional alkali precipitation method with a microalgae treatment system is a highly efficient approach for the removal of excess nutrients from hydroponic greenhouse effluent in a short treatment time. The microalgae can provide a source of value in the form of carotene. © 2012 Society of Chemical Industry
Color Doppler ultrasound‐guided transducer compression of post biopsy bleeding of kidney transplants
Authors: Brabrand Knut , Midtvedt Karsten , Günther Anne , Marthinsen Pål Bache , Berstad Audun Elnaes 
Published: August 2012

AbstractBackground.Color Doppler ultrasound (CDUS) has a potential of early detection of post biopsy bleeding. We describe CDUS guidance in planning, acquisition, and, in the case of bleeding, compression of the needle tract in biopsy procedures of kidney transplants.Methods.Eighty‐three kidney transplant biopsy procedures performed on clinical indication were performed in 71 adult patients, 25 women, mean age 51 years, using CDUS and 18‐G biopsy needles. Bleeding needle tracts were compressed using CDUS guidance.Results.CDUS immediately detected blood leakage and facilitated compression of the bleeding needle tract in 34 (41%) of the 83 procedures, including 53 (25%) of 215 needle passes. In 34 bleeding procedures, the mean duration of the longest bleeding time after a needle pass was 124 seconds (median, 20 seconds; range, 3–1440 seconds). In 12 of these procedures (35%), the bleeding time was 30 seconds or more. In six procedures (18%), a bleeding of 120 seconds or more was observed. Complications included seven small hematomas. Five hematomas developed in procedures where the longest duration CD bleeding was 120 seconds or more.Conclusions.CDUS detects bleeding and facilitates direct transducer compression of the needle tract in a substantial portion of biopsy procedures of kidney transplants. Only minor hematomas occurred.
© 2012 Wiley Periodicals, Inc. J Clin Ultrasound 2013