bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruption of GIP/GIPR Axis in Human Adipose Tissue Is Linked to Obesity and Insulin Resistance
Authors: Ceperuelo-Mallafré Victòria , Duran Xavier , Pachón Gisela , Roche Kelly , Garrido-Sánchez Lourdes , Vilarrasa Nuria , Tinahones Francisco J. , Vicente Vicente , Pujol Jordi , Vendrell Joan , Fernández-Veledo Sonia 
Published: February 2014

Context:Glucose-dependent insulinotropic peptide (GIP) has a central role in glucose homeostasis through its amplification of insulin secretion; however, its physiological role in adipose tissue is unclear.Objective:Our objective was to define the function of GIP in human adipose tissue in relation to obesity and insulin resistance.Design:GIP receptor (GIPR) expression was analyzed in human sc adipose tissue (SAT) and visceral adipose (VAT) from lean and obese subjects in 3 independent cohorts. GIPR expression was associated with anthropometric and biochemical variables. GIP responsiveness on insulin sensitivity was analyzed in human adipocyte cell lines in normoxic and hypoxic environments as well as in adipose-derived stem cells obtained from lean and obese patients.Results:GIPR expression was downregulated in SAT from obese patients and correlated negatively with body mass index, waist circumference, systolic blood pressure, and glucose and triglyceride levels. Furthermore, homeostasis model assessment of insulin resistance, glucose, and G protein-coupled receptor kinase 2 (GRK2) emerged as variables strongly associated with GIPR expression in SAT. Glucose uptake studies and insulin signaling in human adipocytes revealed GIP as an insulin-sensitizer incretin. Immunoprecipitation experiments suggested that GIP promotes the interaction of GRK2 with GIPR and decreases the association of GRK2 to insulin receptor substrate 1. These effects of GIP observed under normoxia were lost in human fat cells cultured in hypoxia. In support of this, GIP increased insulin sensitivity in human adipose-derived stem cells from lean patients. GIP also induced GIPR expression, which was concomitant with a downregulation of the incretin-degrading enzyme dipeptidyl peptidase 4. None of the physiological effects of GIP were detected in human fat cells obtained from an obese environment with reduced levels of GIPR.Conclusions:GIP/GIPR signaling is disrupted in insulin-resistant states, such as obesity, and normalizing this function might represent a potential therapy in the treatment of obesity-associated metabolic disorders.
Expression and Prognostic Significance of APE1/Ref-1 and NPM1 Proteins in High-Grade Ovarian Serous Cancer
Authors: Londero Ambrogio P. , Orsaria Maria , Tell Gianluca , Marzinotto Stefania , Capodicasa Valentina , Poletto Mattia , Vascotto Carlo , Sacco Cosimo , Mariuzzi Laura 
Published: February 2014

Abstract

Objectives:
To correlate the expression profile of human apurinic endonuclease/redox factor 1 (APE1/Ref-1) with that of nucleolar/nucleoplasmic protein nucleophosmin 1 (NPM1) in association with the aggressiveness and progression of high-grade ovarian serous cancer.

Methods:
Retrospective study analyzing a tissue microarray of 73 women affected by high-grade ovarian serous cancer. Protein expression was assessed by immunohistochemistry on primary tumor masses and synchronous peritoneal metastases if present.

Results:
APE1/Ref-1 and NPM1 showed a significant correlation in ovarian serous cancer. Patients with a poorer outcome showed a significant overexpression of nuclear NPM1 protein. A Cox proportional hazards multivariate regression model revealed NPM1 expression to be independently significant for overall survival in high-grade ovarian serous cancers after correcting for stage, age, cytoreduction completeness, and platinum resistance.

Conclusions:
APE1/Ref-1 interacts with NPM1 to control the DNA damage repair system, and it is likely that this interaction plays a defining role in high-grade ovarian serous carcinoma. A high NPM1 immunohistochemical expression was independently correlated with a shorter survival period and thus appears to be an important prognostic factor.
Beam shaping of laser diode stacks for compact and efficient illumination devices at the French-German Research Institute of Saint-Louis
Authors: Lutz Yves , Laurenzis Martin 
Published: February 2014

Abstract
Laser diode stacks are applied for the realization of compact and efficient laser illumination devices for active imaging (e.g., laser gated viewing). An efficient use of illumination power and sensor arrays has to adapt the laser illumination to the sensor’s field of view (FOV) with a perfectly matched and homogeneous illumination field. In the past decades, ISL has studied different methods for beam shaping and homogenization of laser diode stacks. In this publication, the authors give a review of the development of different beam-shaping techniques for auto-stack, minibar stack in a specific configuration and standard laser diode stacks. The presented methods are based on the application of wedge-type waveguides, fast axis and slow axis collimation, and the rearrangement of the laser beams by polarization overlapping and virtual restacking. All presented methods have very high transmission efficiencies (>80%) and lead to a homogeneous illumination matched to the sensor’s field of view.
The interrelation between microfibril angle (MFA) and hygrothermal recovery (HTR) in compression wood and normal wood of Sugi and Agathis
Authors: Tanaka Midori , Yamamoto Hiroyuki , Kojima Miho , Yoshida Masato , Matsuo Miyuki , Lahjie Abubakar M. , Hongo Ichiro , Arizono Takeshi 
Published: February 2014

Abstract
Tree growth stress (GS) consists of an elastic component and a viscoelastic locked-in component. The elastic component is released instantaneously by cutting wood, whereas the locked-in component remains. The latter can be released by hot water treatment, which is known as hygrothermal recovery (HTR). In this paper the mechanism behind HTR is described and interpreted in terms of the microfibril angle (MFA) in the cell wall as follows: during cell-wall maturation, axial tensile stress is generated in the cellulose microfibrils (CMF), whereas isotropic compressive stress is generated in the matrix of lignin-hemicellulose (MT). Some amount of microscopic stresses remains following the removal of the wood from the living stem. Hygrothermal (HT) treatment induces recovery of remaining compressive stress in the MT, which causes its expansion. Axial tensile stress in the CMF are released by HT softening of the MT. This causes the CMF to contract along its length and to expand laterally. The combined effect of the expansions of the MT and contraction of the CMF causes the wood to deform anisotropically. This is the HTR of wood. The degree of anisotropy is determined by the MFA on the basis of reinforced-matrix theory.
Energy metabolism and hindbrain AMPK: regulation by estradiol
Authors: Briski Karen P. , Ibrahim Baher A. , Tamrakar Pratistha 
Published: February 2014

Abstract
Nerve cell energy status is screened within multiple classically defined hypothalamic and hindbrain components of the energy balance control network, including the hindbrain dorsal vagal complex (DVC). Signals of caudal DVC origin have a physiological role in glucostasis, e.g., maintenance of optimal supply of the critical substrate fuel, glucose, through control of motor functions such as fuel consumption and gluco-counterregulatory hormone secretion. A2 noradrenergic neurons are a likely source of these signals as combinatory laser microdissection/high-sensitivity Western blotting reveals expression of multiple biomarkers for metabolic sensing, including adenosine 5′-monophosphate-activated protein kinase (AMPK). Hypoglycemia elicits estradiol-dependent sex differences in A2 AMPK activation as phospho-AMPK (pAMPK) expression is augmented in male and ovariectomized (OVX) female, but not estrogen-replaced, OVX rats. This dichotomy may reflect, in part, estradiol-mediated up-regulation of glycolytic and tricarboxylic acid cycle enzyme expression during hypoglycemia. Our new model for short-term feeding abstinence has physiological relevance to planned (dieting) or unplanned (meal delay) interruption of consumption in modern life, which is negatively correlated with appetite control and obesity, and is useful for investigating how estrogen may mitigate the effects of disrupted fuel acquisition on energy balance via actions within the DVC. Estradiol reduces DVC AMPK activity after local delivery of the AMP mimic, 5-aminoimidazole-4-carboxamide-riboside, or cessation of feeding for 12 h but elevates pAMPK expression when these treatments are combined. These data suggest that estrogen maintains cellular energy stability over periods of suspended fuel acquisition and yet optimizes, by DVC AMPK-dependent mechanisms, counter-regulatory responses to metabolic challenges that occur during short-span feeding abstinence.
Speaker Verification Under Degraded Conditions Using Empirical Mode Decomposition Based Voice Activity Detection Algorithm
Authors: Rudramurthy M. S. , Prasad V. Kamakshi , Kumaraswamy R. 
Published: February 2014

AbstractThe performance of most of the state-of-the-art speaker recognition (SR) systems deteriorates under degraded conditions, owing to mismatch between the training and testing sessions. This study focuses on the front end of the speaker verification (SV) system to reduce the mismatch between training and testing. An adaptive voice activity detection (VAD) algorithm using zero-frequency filter assisted peaking resonator (ZFFPR) was integrated into the front end of the SV system. The performance of this proposed SV system was studied under degraded conditions with 50 selected speakers from the NIST 2003 database. The degraded condition was simulated by adding different types of noises to the original speech utterances. The different types of noises were chosen from the NOISEX-92 database to simulate degraded conditions at signal-to-noise ratio levels from 0 to 20 dB. In this study, widely used 39-dimension Mel frequency cepstral coefficient (MFCC; i.e., 13-dimension MFCCs augmented with 13-dimension velocity and 13-dimension acceleration coefficients) features were used, and Gaussian mixture model–universal background model was used for speaker modeling. The proposed system’s performance was studied against the energy-based VAD used as the front end of the SV system. The proposed SV system showed some encouraging results when EMD-based VAD was used at its front end.
Thermotropic overheating protection glazings: effect of functional additives and processing conditions on the overheating protection performance
Authors: Weber Andreas , Resch Katharina 
Published: February 2014

Abstract
Within this study, the effect of functional additives and processing conditions on the overheating protection performance of thermotropic systems with fixed domains (TSFD) for overheating protection purposes was evaluated. The focus was on improving the overheating protection performance of a prototype TSFD based on a UV curable acrylate resin by optimization of the material constitution (addition of functional additives like surfactants and nucleating agents) and the processing conditions (temperature conditions during manufacturing, annealing). For the evaluated system, an effect of the nucleating agent on the overheating protection performance was ascertained. Furthermore, omission of an annealing step improved the overheating protection performance slightly.
Growth of Bi2Se3 Films by Chemical Bath Deposition at Room Temperature
Authors: Phok Sovannary , Al Wahshi Fatima Subait , Al Baity Shifaa Mohsen , Abdulla Yalyali Saeed Ali 
Published: February 2014

ABSTRACTBismuth selenide (BixSey) films are deposited onto glass substrate using chemical bath deposition at room temperature. The reacting bath contained bismuth nitrate, triethanolamine and sodium selenosulfate as selenium (Se) source. Ammonium hydroxide is used to adjust the pH of the bath. The films deposited in solutions containing Se source solution of 10 ml and 15 ml are characterized by surface morphological, compositional and structural, properties. The optimum deposition time is about 3 hours for both solutions. Films deposited up to 24 hours in bath with 10 ml Se source solution had thickness ranging up to 232 nm. The deposition rate is found to increase up to 61 nm/h for 3-hour deposition. In the case of bath with 15 ml Se source solution, the film thickness ranged from 45 nm to 632 nm for 1-hour to 24-hour deposition, respectively; with a deposition rate increasing up to 123 nm/h for 3-hour deposition. Film roughness of about 6.6 nm to 22.8 nm is measured by atomic force microscope for films deposited in bath containing 10 mL Se source and 15 ml of Se source, respectively. Crack free layers are observed with randomly large plate-like particles on top of the layer for some films. The films with typical composition of Bi21.8Se78.2 are found to be rich in Se when deposited for 6 hours, whereas the composition of a film deposited in the same bath (10 mL Se source) for 3 hours is found at Bi60.3Se39.6. Additionally, structural analysis performed by x-ray diffraction (XRD) did not reveal well-defined XRD patterns, which indicates that BixSey films were constituted mostly of nanocrystalline grains.
Electrical Characteristics of TiW/ZnO Schottky contact with ALD and PLD
Authors: Shen Mei , Afshar Amir , Gupta Manisha , Shoute Gem , Cadien Ken , Tsui Ying Yin , Barlage Doug 
Published: February 2014

ABSTRACTAn electrical and analytical study was carried out to investigate TiW/ZnO Schottky contacts with 30 nm ZnO thin film layers deposited by pulsed laser deposition (PLD), plasma enhanced atomic layer deposition (PEALD), and thermal atomic layer deposition (TALD). Devices with ZnO layer deposited by TALD exhibit approximately linear behavior in their I-V measurements. However, both devices with ZnO layers deposited by PEALD and PLD behaved like Schottky rectifiers with barrier heights between TiW and ZnO of 0.51 eV and 0.45 eV respectively and ideality factors of 2.0 and 2.3 respectively. The PEALD deposited ZnO Schotty diodes demonstrated an on/off rectifying ratio of about 25 at ±1 V. The leakage current values of the PLD deposited ZnO Schottky diodes are significantly larger than those of PEALD, leading to a poor on/off rectifying ratio of ∼4. Due to the small thickness, a critical breakdown strength of 1.3 MV/cm was estimated for PEALD-ZnO thin films.
A 2D Covalently Bound Continuous Protein Gradient Assay
Authors: Mintz Benjamin , Cooper James A. 
Published: February 2014

ABSTRACTTraditional methods of quantifying cell movement in response to a chemotactic factors provide either a binary count of cell migration in response to a known concentration of the factor of interest in solution, as in Boyden chamber assays, or a method of tracking cells to determine velocities across a solubilized protein gradient where exact concentrations vary over time and are difficult to define, as in the Ibidi chemotaxis gradient assay. Using a silane self-assembling monolayer (SAM)-based procedure pioneered by V Hlady and associates, we have developed an assay capable of covalently binding a wide variety of proteins to an optically transparent surface in a 2D pattern via amine linkages. The pattern was then verified by contact angle and Raman and X-ray photoelectron spectroscopy. This new assay provides greater control of protein concentration and gradient intensity than when using only solubilized proteins.
Variability in spectral characteristics of trampled high-mountain grasslands
Authors: Kycko Marlena , Zagajewski Bogdan , Kozłowska Anna 
Published: February 2014

Abstract
The goal of the paper is a presentation of field remote sensing methods for the analysis of the trampled plants of a highly protected mountain meadow ecosystem (M&B UNESCO Reserve and one of the most important Polish National Parks). The research area covers a core part of the Western Tatras - the Gąsienicowa Valley and Kasprowy Wierch summit, which are among the most visited destinations of the Polish Tatras. The research method is based on field hyperspectral measurements, using the ASD FieldSpec 3 spectrometer, on the dominant plant species of alpine swards. Sampling sites were located on trampled areas (next to trails) and reference plots, with the same species, but located more than 10 m from the trail (where the probability of trampling was very low, but the same composition of analysed plants). In each case, homogenous plots with a domination of one plant species were investigated. Based on the hyperspectral measurements, spectral characteristics as well as vegetation indices were analysed with the ANOVA statistical test. This indicated a varied resistance to trampling of the studied plant species. The analysis of vegetation indices enabled the selection of those groups which are the most useful for research into mountain vegetation condition: the broadband greenness group; the narrowband greenness group, measuring chlorophyll content and cell structure; and the canopy water content group. The results of the analyses show that vegetation of the High Tatras is characterised by optimal ranges of remote sensing indices. Only plants located nearest to the trails were in a worse condition (chlorophyll and water content was lower for the reference targets). These differences are statistically significant, but the measured values indicate a good condition of vegetation along trampled trails, within the range of optimum plant characteristics.
Cryptic diversity in a cosmopolitan diatom known as Asterionellopsis glacialis (Fragilariaceae): Implications for ecology, biogeography, and taxonomy
Authors: Kaczmarska Irena , Mather Laura , Luddington Ian A. , Muise France , Ehrman James M. 
Published: February 2014

• Premise of the study: Diatoms have long been known as the most species‐rich of algal groups, with a wide range of estimates for species number (20–200 k) due to differing species concepts. The fine valve structure in Asterionellopsis glacialis, a diatom believed cosmopolitan and eurytopic, has never been systematically examined using modern microscopy and is an excellent candidate to genetically test morphology‐based conspecificity among its geographically distant culture isolates.• Methods: Isolates from the Atlantic and Pacific Oceans that were morphologically delineated as A. glacialis were genetically characterized using three nuclear DNA regions (two 18S rDNA fragments and ITS region) and one plastidal (rbcL) and one mitochondrial gene (cox1) and related to SEM‐based morphometrics.• Key results: Five genetically distinct groupings were found, four of which are new to science. ITS2 RNA transcript secondary structure was species specific as were plastidal and mitochondrial genes, while the 18S gene fragments did not diverge sufficiently to segregate new species efficiently. We genetically circumscribed the A. glacialis epitype.• Conclusions: The morphological diversification of the species examined in this study lags behind their genetic divergence. The currently accepted 2% cutoff level of operational taxonomic unit (OTU) clustering in 18S rDNA environmental sequencing is too high to recognize genetic diversity in Asterionellopsis and very likely in many other species. Our results support the notion that a considerable number of species and diversity remain to be discovered among diatoms and that species number may be more in line with higher estimates. Molecular signatures of the species discovered here will aid in their globally consistent identification and ultimate understanding of their ecology.
Education and Knowledge Production in Workers’ Struggles: Learning to resist, learning from resistance
Authors: Bleakney David , Choudry Aziz 
Published: February 2014

Trade unions and other sites of community-labour organizing such as workers centres are rich, yet contested spaces of education and knowledge production in which both non-formal and informal / incidental forms of learning occur. Putting forward a critique of dominant strands of worker education, the authors ask what spaces exist for social movement knowledge production in these milieus? This article critically discusses the prospects, tensions and challenges for effective worker education practice in trade unions, alongside a discussion of informal learning and knowledge production in migrant and immigrant worker organizing. We consider how worker education practices within trade unions might best be built to support critical thinking, the collective power of working people and cultures of resistance, and the significance of knowledge and learning in community-labour struggles.
Human papillomavirus and p53 expression in cancer of unknown primary in the head and neck region in relation to clinical outcome
Authors: Sivars Lars , Näsman Anders , Tertipis Nikolaos , Vlastos Andrea , Ramqvist Torbjörn , Dalianis Tina , Munck‐Wikland Eva , Nordemar Sushma 
Published: February 2014

AbstractPatients with cancer of unknown primary (CUP) in the head neck region are generally treated with neck dissection followed by radiotherapy at times combined with chemotherapy, a treatment associated with considerable side effects. Some of these tumors may originate as human papillomavirus (HPV)‐positive oropharyngeal squamous cell carcinoma (OSCC), with better clinical outcome than head neck squamous cell cancer (HNSCC) in general, and could potentially do well with less treatment. Here, we therefore investigated whether HPV status and p53‐expression correlated to clinical outcome in patients with CUP in the head neck region. Fifty metastases were analyzed for presence of HPV DNA, and expression of p16INK4A and p53 and the data were correlated to clinical outcome. Patients with HPV DNA‐positive (HPVDNA+) metastases had significantly better 5‐year overall survival (OS) compared to those with HPVDNA− metastases (80.0% vs. 36.7%, respectively; P = 0.004), with a similar tendency for disease‐free survival (DFS). These survival rates showed excellent concordance with those of HPVDNA+ and HPVDNA− OSCC in Sweden during the same time period, strengthening the hypothesis that HPVDNA+ head and neck CUP may originate from HPVDNA+ OSCC. In addition, having absent/intermediary‐low as compared to high expression of p53 correlated to a better prognosis with a 69% as compared to 14% 5‐year OS, respectively (P < 0.001), and for DFS the tendency was analogous. In conclusion, both HPV status and p53 expression are valuable prognostic factors in patients with CUP in the head and neck region and should be further explored for clinical use.
Determination of the Absolute Stereochemistry of Two New Aristophyllene Sesquiterpenes: A Combined Theoretical and Experimental Investigation
Authors: Wu Rui‐Bo , Cheng Zhong‐Bin , Han Qing‐Hua , Lin Ting‐Ting , Zhou Jing‐Wei , Tang Gui‐Hua , Yin Sheng 
Published: February 2014

ABSTRACTAristoyunnolins G (1) and H (2), two new diastereoisomeric sesquiterpenes featuring a rare aristophyllene skeleton, were isolated from the traditional Chinese medicine Aristolochia yunnanensis. Their absolute stereochemistry involving three chiral centers was determined by combined chemical, spectral, and Density Functional Theory (DFT) calculation methods. Chirality 26:189–193, 2014. © 2014 Wiley Periodicals, Inc.