bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrochemical Biosensors Based on Native DNA and Nanosized Mediator for the Detection of Anthracycline Preparations
Authors: Evtugyn Gennady , Porfireva Anna , Stepanova Veronika , Budnikov Herman 
Published: January 2015

AbstractA novel electrochemical DNA‐sensor based on glassy carbon electrode (GCE) modified with electropolymerized Neutral red (NR) and polycarboxylated thiacax[4]arene with covalently attached mediator and electrostatically adsorbed DNA was developed for sensitive detection of anthracycline preparations. Intercalation of doxorubicin, daunorubicin and idarubicin into DNA increases the charge transfer resistance and decreases the electron exchange resulted in decay of the cathodic peak of NR reduction. The DNA‐sensor developed makes it possible to determine down to 0.05 nM doxorubicin, o.1 nM doxorubicin and 0.5 nM idarubicin. The DNA‐sensor was tested on detection of doxorubicin in pharmaceuticals and artificial blood plasma with 95–100 % recovery.
CC Bonding of Graphene Oxide on 4‐Aminophenyl Modified Gold Electrodes towards Simultaneous Detection of Heavy Metal Ions
Authors: Zhang Yin , Qi Meng , Liu Guozhen 
Published: January 2015

AbstractThis paper studied the electrochemical sensors based on CC bonding of graphene oxide (GO) on π‐conjugated aromatic group modified gold electrodes for simultaneous detection of heavy metal ions. For comparison, another sensing interface Au‐Ph‐NH‐CO‐GO, in which GO was modified to Au‐Ph‐NH2 interfaces by amide bonding. On the basis of the principle of heavy metal ions complexation with oxygenated species on GO, the fabricated sensing interfaces were used for the simultaneous determination of Pb2+, Cu2+ and Hg2+. The performance of two sensing interfaces for simultaneous detection of three metal ions was compared. Au‐Ph‐GO sensing interface demonstrated higher sensitivity and better repeatability than Au‐Ph‐NH‐CO‐GO sensing interface.
Electrochemical and Photometric Detection of Plasmin by Specific Peptide Substrate
Authors: Castillo Gabriela , Pribransky Kinga , Mező Gábor , Kocsis László , Csámpai Antal , Németh Krisztina , Keresztes Zsófia , Hianik Tibor 
Published: January 2015

AbstractWe report a comparative analysis of plasmin detection by means of electrochemical biosensor and photometric assays. The biosensor has been based on ferrocene‐modified peptide (Fc‐P) specific to plasmin. Fc‐P substrate is enzymatically cleaved by plasmin and the short fragment of peptide contained Fc is released causing decrease of redox current measured by cyclic voltammetry. The biosensor was tested in the range 1–20 nM of freshly prepared plasmin and it was highly selective (LOD=0.56±0.03 nM) demonstrating lower reaction to other proteins such as thrombin and β‐casein. The concentration of plasmin has been determined also by a photometric analysis based on UV‐vis spectroscopy (LOD=3.68±0.04 nM). The assay has been validated in milk samples.
Study of the specific heat capacity of biomass from banana waste for application in the second‐generation ethanol industry
Authors: Villa‐Vélez Harvey A. , Váquiro Henry A. , Lopes‐Filho José F. , Telis Vânia R. N. , Telis‐Romero Javier 
Published: January 2015

An accurate determination of the thermal properties of biomass is particularly important when studying biomaterials for the second‐generation ethanol industry. This study presents the thermograms of aqueous solutions of banana flower stalks obtained using differential scanning calorimetry. The samples were prepared using a detailed procedure to obtain normalized specific heat values as a function of temperature, pH, and biomass concentration. The specific heat capacity data were fitted using empirical first‐order equations with an algorithm for nonlinear regression, followed by a statistical analysis. All the models applied in modeling of the specific heat capacity showed statistically significant goodness of fit (
> 0.996) for all the parameters in the selected expressions. The results obtained in this research can provide information for the modeling, design, and control of the unit operations involved in the second‐generation ethanol industry. © 2015 American Institute of Chemical Engineers Environ Prog, 34: 1221–1228, 2015
The European Initiative ProYouth for the Promotion of Mental Health and the Prevention of Eating Disorders* Screening Results in Hungary
Authors: Szabó Kornélia , Czeglédi Edit , Babusa Bernadett , Szumska Irena , Túry Ferenc , Sándor Imola , Bauer Stephanie 
Published: January 2015

AbstractObjectiveThe ProYouth programme focuses on the promotion of mental health and the prevention of eating disorders (EDs) among young people. The aim of our study was to explore whether the programme can address individuals who are at risk for developing 2EDs.MethodThis study is designed as an online cross‐sectional survey (n = 664, 12.2% men, 87.8% women, mean age: 24.9 years, SD = 5.4 years, range: 18–40 years). Measures included demographic data, self‐reported weight and height, the Patient Health Questionnaire for Depression and Anxiety, Short Evaluation of Eating Disorders, Weight Concerns Scale and previous/current treatment for EDs.ResultsIn terms of severity of EDs, 22.9% (n = 152) of the screened participants were symptom free, 48.8% (n = 324) had considerable concerns about their weight, 11.1% (n = 74) were slightly impaired, 15.1% (n = 100) had severe impairment and 2.1% (n = 14) of participants are currently under treatment for EDs. In total, 56.3% of users (n = 374) registered in the programme. According to our results, those who had considerable concerns about their weight and individuals who were severely impaired registered with a greater odds to the programme than those who were symptom free [odds ratio (OR) = 1.64, p = .021 and OR = 1.90, p = .023, respectively]. Furthermore, those who previously received treatment for their ED registered to the programme with greater odds than those who did not (OR = 2.40, p = .017).ConclusionProYouth successfully addressed those who have elevated concerns about their weight and who also registered with greater odds to the programme than those who were symptom free regarding EDs. The screening results show that there is a greater need for specialized care targeting EDs in Hungary than what is currently available. Copyright © 2015 John Wiley & Sons, Ltd and Eating Disorders Association.
Determination of biogenic amines by high‐performance liquid chromatography (HPLC‐DAD) in probiotic cow's and goat's fermented milks and acceptance
Authors: Costa Marion P. , Balthazar Celso F. , Rodrigues Bruna L. , Lazaro Cesar A. , Silva Adriana C. O. , Cruz Adriano G. , Conte Junior Carlos A. 
Published: January 2015

AbstractThis study evaluated the presence of biogenic amines in fermented cow's and goat's milks containing probiotic bacteria, during the first 10 days of chilled storage (4 ± 2°C), when the probiotic strains are most viable. The overall acceptance of both fermented milks, produced using the same starter culture and probiotics, was tested. In both products, the initially high levels of tyramine (560 mg kg−1means for both fermented milks), the predominant biogenic amine, increased during the storage period, which may be considered this amine as a quality index for fermented milks. The other principal biogenic amines (putrescine, cadaverine, histamine, and spermidine) were produced on days 1–5 of storage, and thereafter decreased. At the end of the 10th day, these amines, respectively, showed values of fermented cow's milk 20.26, 29.09, 17.97, and 82.07 mg kg−1; and values of fermented goat's milk 22.92, 29.09, 34.85, and 53.85 mg kg−1, in fermented cow's and goat's milk. Fermented cow's milk was well accepted compared to fermented goat's milk. The results suggested that the content of biogenic amines may be a criterion for selecting lactic acid bacteria used to produce fermented milks.
Endocrine‐disrupting potentials of equine estrogens equilin, equilenin, and their metabolites, in the medaka Oryzias latipes: in silico and DNA microarray studies
Authors: Uchida Masaya , Ishibashi Hiroshi , Yamamoto Ryoko , Koyanagi Akiko , Kusano Teruhiko , Tominaga Nobuaki , Ishibashi Yasuhiro , Arizono Koji 
Published: January 2015

ABSTRACTAlthough several previous studies have demonstrated the presence of equine estrogens in the aquatic environment, limited data are currently available on the endocrine‐disrupting potentials in fish and the risks they pose to aquatic organisms. To investigate the interactions of major equine estrogens equilin (Eq) and equilenin (Eqn), as well as their metabolites 17α‐dihydroequilin, 17β‐dihydroequilin, 17α‐dihydroequilenin and 17β‐dihydroequilenin, with the estrogen receptor α (ERα) of medaka (Oryzias latipes), a three‐dimensional model of the ligand‐binding domain (LBD) of ERα was built in silico, and docking simulations were performed. The docking simulation analysis indicated that the interaction of 17β‐dihydroequilenin with the ERα LBD is the most potent, followed by those of 17α‐dihydroequilin and 17β‐dihydroequilin, whereas those of Eq and Eqn were least potent. We further analyzed gene expression profiles in the livers of male medaka exposed to Eq and Eqn. A DNA microarray representing 6000 genes revealed that 24‐h exposure to Eq and Eqn (100 ng/L) upregulated the expression of 6 and 34 genes in the livers of males, respectively. Genes upregulated by Eq included the estrogenic biomarker genes vitellogenins and choriogenins, suggesting the estrogenic potential of Eq. In contrast, Eqn exposure upregulated several cancer‐related genes, such as mediator complex subunit 16 and RAS oncogene family members, suggesting a carcinogenic potential for Eqn. These results suggest that equine estrogens may have not only endocrine‐disrupting potentials via the ERα signaling pathway but also carcinogenic potency in male medaka. Copyright © 2015 John Wiley & Sons, Ltd.
Nanobiocomposite of poly(lactide‐co‐glycolide)/chitosan electrospun scaffold can promote proliferation and transdifferentiation of Schwann‐like cells from human adipose‐derived stem cells
Authors: Razavi Shahnaz , Zarkesh‐Esfahani Hamid , Morshed Mohammad , Vaezifar Sedigheh , Karbasi Saeed , Golozar Mohammad Ali 
Published: January 2015

AbstractThe transdifferentiation of human adipose‐derived stem cells (ADSCs) into Schwann‐like cells on biocomposite scaffolds may be a critical issue in nerve regeneration medicine. In this study, tissue‐engineered scaffold with chitosan (CS) nanopowders and poly(lactide‐co‐glycolide) (PLGA) was investigated for its potential Schwann cells (SCs) transdifferentiation.The differentiation of human ADSCs into S‐like cells was induced with different CS content and direction of nanofibers on PLGA/CS scaffolds. Cell morphology and proliferation of differentiated cells were investigated by scanning electron microscopy and 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl tetrazolium bromide assay respectively. For assessment efficiency of transdifferentiation, the expression of SC markers (glial fibrillary acidic protein and S100), and myelinogenic marker (myelin basic protein) was investigated in different nanochitosan content and direction of nanofibers scaffolds, using immunocytochemistry technique.The nanochitosan can significantly promote cell proliferation of differentiated cells (p < 0.05). The mean percentage of S‐like cells on greater CS content nanofibers scaffold was significantly higher than others (p < 0.05). In addition, the align orientation of nanofibers in scaffolds guided the differentiation of ADSCs toward myelinating S‐like cells on the constructs.Overall, we found that high CS content and aligned‐orientation of nanofibers in biocomposite scaffold (70/30A) can promote differentiation and myelinogenic capacity of S‐like cells induced from human ADSCs. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 2628–2634, 2015.
Performance Evaluation of a Salivary Amylase Biosensor for Stress Assessment in Military Field Research
Authors: Peng Henry T. , Savage Erin , Vartanian Oshin , Smith Shane , Rhind Shawn G. , Tenn Catherine , Bjamason Stephen 
Published: January 2015

BackgroundA convenient biosensor for real‐time measurement of biomarkers for in‐field psychophysiological stress research and military operations is desirable. We evaluated a hand‐held device for measuring salivary amylase as a stress marker in medical technicians undergoing combat casualty care training using two different modalities in operating room and field settings.MethodsSalivary amylase activity was measured by two biosensor methods: directly sampling saliva with a test strip placed under the tongue or pipetting a fixed volume of precollected saliva onto the test strip, followed by analyzing the sample on the strip using a biosensor. The two methods were compared for their accuracy and sensitivity to detect the stress response using an enzyme assay method as a standard.ResultsThe measurements from the under‐the‐tongue method were not as consistent with those from the standard assay method as the values obtained from the pipetting method. The under‐the‐tongue method did not detect any significant increase in the amylase activity due to stress in the operating room (P > 0.1), in contrast to the significant increases observed using the pipetting method and assay method with a significance level less than 0.05 and 0.1, respectively. Furthermore, the under‐the‐tongue method showed no increased amylase activity in the field testing, while both the pipetting method and assay method showed increased amylase activity in the same group (P < 0.1).ConclusionThe accuracy and consistency of the biosensors need to be improved when used to directly measure salivary amylase activity under the tongue for stress assessment in military medical training.
Randomized clinical trial of peritoneal lavage for preventing surgical site infection in elective liver surgery
Authors: Tanaka Kuniya , Matsuo Kenichi , Kawaguchi Daisuke , Murakami Takashi , Hiroshima Yukihiko , Hirano Atsushi , Sato Sho , Endo Itaru , Taguri Masataka , Koda Keiji 
Published: January 2015

AbstractBackgroundAlthough intraoperative peritoneal lavage is often performed routinely with the aim of reducing peritoneal contamination, little evidence of lavage benefits in elective liver resection without bile duct resection is available. We addressed the issue with a randomized clinical trial.MethodsWe prospectively and randomly assigned consecutive patients undergoing liver resection to a lavage group or a non‐lavage group. Peritoneal lavage was performed at the end of operation for patients in the lavage group. The primary endpoint was the rate of surgical site infection.ResultsNinety‐six patients were assigned to the lavage group and 97 to the non‐lavage group. When superficial/deep incisional infection and organ/space infection were considered together, no significant difference in surgical site infection rate was evident between lavage (21.9%) and non‐lavage groups (13.4%, P = 0.135). However, organ/space infection was significantly more frequent in the lavage group (16.7%) than the non‐lavage group (7.2%, P = 0.048). Peritoneal lavage was identified as a risk factor for organ/space infection by multivariate analysis (relative risk, 2.977; confidence interval, 1.094 to 8.100; P = 0.033).ConclusionIntraoperative peritoneal lavage does not reduce overall incidence of surgical site infection and may increase risk of organ/space infection.
An intercomparison of CMIP5 and CMIP3 models for interannual variability of summer precipitation in Pan‐Asian monsoon region
Authors: Gao Ya , Wang Huijun , Jiang Dabang 
Published: January 2015

ABSTRACTTwenty‐one climate models from Coupled Model Intercomparison Project Phase 3 (CMIP3) and thirty‐one models from the project's Phase 5 (CMIP5) were used to evaluate model reproducibility in assessing interannual variability of summer precipitation in Pan‐Asian monsoon region. The results show that both the multi‐model ensemble means of the best eight models and of the thirty‐one CMIP5 models are more skilful than those of the CMIP3 models in simulating the climatological pattern and the dominant mode of summer precipitation in Pan‐Asian monsoon region. CMIP5 models show improved skill in representing the main characteristic of the first mode of summer precipitation in Pan‐Asian monsoon region, which is a meridional tripole pattern from north to south occurring east of the 80°E region. That is, owing to the improved El Niño–Southern Oscillation (ENSO) pattern and the relationship between Antarctic oscillation in the southern Pacific Ocean (AAOSP) and ENSO, the first dominant mode of summer precipitation in Pan‐Asian monsoon region are captured by CMIP5 models, which indicates that these models are more skilful in simulating the air–sea interaction of the Southern Hemisphere.
Letter: Dual‐mode oxide thin film transistor shift register for external compensation in active‐matrix organic light emitting diode displays
Authors: Song Seok‐Jeong , Nam Hyoungsik 
Published: January 2015

AbstractThis letter describes a dual‐mode oxide thin film transistor shift register for active‐matrix organic light emitting diode displays, which provides two separate scanning and sensing pulses on one line. At a 240 Hz full‐high definition (HD) timing, estimated power consumptions are 2.44 and 2.81 mW for scanning and sensing shift registers of 16 stages at VTH = −1.56 V, respectively.
Menstrual Cycle Effects on Psychological Symptoms in Women With PTSD
Authors: Nillni Yael I. , Pineles Suzanne L. , Patton Samantha C. , Rouse Matthew H. , Sawyer Alice T. , Rasmusson Ann M. 
Published: January 2015

AbstractThe menstrual cycle has been implicated as a sex‐specific biological process influencing psychological symptoms across a variety of disorders. Limited research exists regarding the role of the menstrual cycle in psychological symptoms among women with posttraumatic stress disorder (PTSD). The current study examined the severity of a broad range of psychological symptoms in both the early follicular (Days 2–6) and midluteal (6–10 days postlutenizing hormone surge) phases of the menstrual cycle in a sample of trauma‐exposed women with and without PTSD (N = 49). In the sample overall, total psychological symptoms (d = 0.63), as well as depression (d = 0.81) and phobic anxiety (d = 0.81) symptoms, specifically, were increased in the early follicular compared to midluteal phase. The impact of menstrual cycle phase on phobic anxiety was modified by a significant PTSD × Menstrual Phase interaction (d = 0.63). Women with PTSD reported more severe phobic anxiety during the early follicular versus midluteal phase, whereas phobic anxiety did not differ across the menstrual cycle in women without PTSD. Thus, the menstrual cycle appears to impact fear‐related symptoms in women with PTSD. The clinical implications of the findings and future research directions are discussed.
A Novel Laser Tool for High‐Volume Sample Preparation
Authors: Höche Thomas , Krause Michael , Ebert Martin , Wagner Uwe , Gebhardt Mandy 
Published: January 2015

AbstractOver the past fifty years, lasers have perpetuated to find new, often groundbreaking applications in science and technology. The most important features of lasers are that photons are inherently free of elemental contamination, extremely high energy densities can be focused in very small areas and the laser beam can be precisely positioned using deflection mirrors. By reducing pulse lengths from a few nanoseconds down to the picosecond or femtosecond range, materials ablation is becoming increasingly “athermal”, i. e., structure damage by local heating is reduced to well below a few microns. In view of these outstanding characteristics of lasers as tools for micromachining, it is very surprising that sample preparation for microstructure diagnostics so far hasn't made use of laser technology.
Highly‐Efficient Semiconductor Laser Diodes
Authors: Zorn Martin 
Published: January 2015

AbstractSemiconductor laser diodes manufactured as laser bars, laser arrays, and single emitters are highly‐desired light sources, e. g. for direct material processing, as pump sources for solid state and fiber lasers or in medical applications. They feature a high output power and efficiency combined with a good reliability resulting in a long‐living device with a low cost of ownership. Desired wavelengths for these applications range from 760 nm, e. g. as replacement for Alexandrite lasers in esthetic skin treatment, up to 1,060 nm for direct material processing and pumping applications.
The extracellular RNA complement of Escherichia coli
Authors: Ghosal Anubrata , Upadhyaya Bimal Babu , Fritz Joëlle V. , Heintz‐Buschart Anna , Desai Mahesh S. , Yusuf Dilmurat , Huang David , Baumuratov Aidos , Wang Kai , Galas David , Wilmes Paul 
Published: January 2015

AbstractThe secretion of biomolecules into the extracellular milieu is a common and well‐conserved phenomenon in biology. In bacteria, secreted biomolecules are not only involved in intra‐species communication but they also play roles in inter‐kingdom exchanges and pathogenicity. To date, released products, such as small molecules, DNA, peptides, and proteins, have been well studied in bacteria. However, the bacterial extracellular RNA complement has so far not been comprehensively characterized. Here, we have analyzed, using a combination of physical characterization and high‐throughput sequencing, the extracellular RNA complement of both outer membrane vesicle (OMV)‐associated and OMV‐free RNA of the enteric Gram‐negative model bacterium Escherichia coli K‐12 substrain MG1655 and have compared it to its intracellular RNA complement. Our results demonstrate that a large part of the extracellular RNA complement is in the size range between 15 and 40 nucleotides and is derived from specific intracellular RNAs. Furthermore, RNA is associated with OMVs and the relative abundances of RNA biotypes in the intracellular, OMV and OMV‐free fractions are distinct. Apart from rRNA fragments, a significant portion of the extracellular RNA complement is composed of specific cleavage products of functionally important structural noncoding RNAs, including tRNAs, 4.5S RNA, 6S RNA, and tmRNA. In addition, the extracellular RNA pool includes RNA biotypes from cryptic prophages, intergenic, and coding regions, of which some are so far uncharacterised, for example, transcripts mapping to the fimA‐fimL and ves‐spy intergenic regions. Our study provides the first detailed characterization of the extracellular RNA complement of the enteric model bacterium E. coli. Analogous to findings in eukaryotes, our results suggest the selective export of specific RNA biotypes by E. coli, which in turn indicates a potential role for extracellular bacterial RNAs in intercellular communication.