bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propagação in vitro de copo-de-leite: sulfato de adenina e 6-benzilaminopurina
Authors: Ribeiro Márcia De Nazaré Oliveira , Pasqual Moacir , Silva Adriano Bortolotti da , Rodrigues Vantuil Antônio 
Published: March 2015

<p> O copo-de-leite [<em>Zantedeschia aethiopica </em>(L.) Spreng.], pertencente à família Araceae, é bastante apreciado tanto como flor de corte quanto na composição de jardins. Entretanto, a propagação convencional dessa espécie apresenta baixo rendimen­to. A cultura de tecidos, além de permitir rápida propagação clonal, também proporciona a obtenção de plantas uniformes e sadias. Sendo assim, o objetivo do trabalho foi estudar a influência de diferentes concentrações de 6-benzilaminopurina (BAP) e sulfato de adenina (SA) sobre o desenvolvimento <em>in vitro </em>de explantes de copo-de-leite. As culturas foram manti­das em meio MS acrescido de BAP (0,0; 8,9; 17,8 e 26,7 μM) e SA (0, 54, 108 e 162 μM) em arranjo fatorial (4x4). O ma­terial vegetal foi cultivado em sala de crescimento com temperatura de 27 ± 1ºC, fotoperíodo de 16 horas, sob intensidade luminosa de 35 μmol m-2 s-1, por período de 60 dias. O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, e a unidade experimental composta de três tubos de ensaio, sendo cada tubo constituído por um explante. A interação significativa entre os fatores BAP e SA ocorreu para número de folhas por explante (4,8) e massa fresca da parte aérea (0,73 g) com 26,7 μM de BAP e 108 μM de SA. Para número de brotos obtidos por explante (2,6) apenas o BAP apresentou significância na concentração de 22,19 μM. Os brotos apresentaram maior comprimento (5,0 cm) na ausência de BAP. A adição de BAP incrementou o número de brotos por explante. O emprego de sulfato de adenina em combinação com BAP teve efeito positivo para o acumulo de massa fresca e numero de folhas no cultivo <em>in vitro</em>.</p>
Estádios de abertura floral e condicionamento em inflorescências de bastão-do-imperador
Authors: Carneiro Daniella Nogueira Moraes , Paiva Patrícia Duarte de Oliveira , Carneiro Leandro Flávio , Rodrigues Roseane De Souza , Lima Luiz carlos De Oliveira , Dias Gabrielen De Maria Gomes , Pedroso Rodolfo Guimarães Alexandre Vasques 
Published: March 2015

<p>As espécies tropicais apresentam grande importância no mercado de flores, mas informações sobre colheita e procedimentos pós colheita ainda são limitados. Para a maioria das espécies tropicais, existem poucas informações sobre soluções a serem utilizadas para condicionamento ou manutenção após a colheita. Assim, objetivou-se avaliar a durabilidade e qualidade pós-colheita de hastes florais de bastão-do-imperador em concentrações de sacarose na solução de condicionamento e diferentes estádios de abertura floral. Hastes florais foram colhidas no início da manhã e tratadas com solução de condicionamento com sacarose nas<br /> concentrações 0 (controle), 10, 20, 30% por 24h, nos três estádios de abertura floral: inflorescências com as brácteas 1/3, 2/3 ou totalmente abertas. O delineamento experimental foi inteiramente casualizado, em esquema fatorial 4x3x6, com 3 repetições em parcela subdividida no tempo. As avaliações foram realizadas em intervalos de 3 dias, determinando-se a massa fresca das hastes florais e avaliação de qualidade das inflorescências por meio de notas, em escala de 0 a 4. Observou-se que a durabilidade<br /> comercial das hastes florais é maior (10 dias) quando as inflorescências são colhidas com 1/3 das brácteas abertas. Recomenda-se a realização de condicionamento em hastes florais de bastão-do-imperador com concentração de 20% de sacarose por 24h para inflorescências colhidas com 1/3 e 2/3 das brácteas abertas. </p>
Avaliação da solução de pulsing com ácido giberélico na vida de vaso de Heliconia bihai
Authors: Guimarães Adriana Andrade , Da Silva Tania Pires , Finger Fernando Luiz , Barbosa José Geraldo 
Published: March 2015

<p>O jasmim-manga (Plumeria spp.) é uma planta muito utilizada na ornamentação urbana, principalmente por sua rusticidade, fácil manejo e exuberância de suas flores. Folhas de Plumeria spp. foram coletadas no município de Dourados, MS, com sintomas e sinais típicos da presença de ferrugem: uredinias pulverulentas de coloração amarelada na face abaxial e manchas cloróticas e necróticas, na face adaxial das folhas, culminando algumas vezes com abscisão foliar. O presente trabalho tem por objetivo registrar a ocorrência da doença no Estado de Mato Grosso do Sul. Observações microscópicas e mensurações de urediniosporos e teliosporos confirmaram a presença do fungo Coleosporium plumeriae infectando essas plantas.</p>
Hypoxia Inducible Factor-1α in Astrocytes and/or Myeloid Cells Is Not Required for the Development of Autoimmune Demyelinating Disease
Authors: Le Moan Natacha , Baeten Kim M. , Rafalski Victoria A. , Kyu Ryu Jae , Rios Coronado Pamela E. , Bedard Catherine , Syme Catriona , Davalos Dimitrios , Akassoglou Katerina 
Published: March 2015

AbstractHypoxia-like tissue alterations, characterized by the upregulation of hypoxia-inducible factor-1α (HIF-1α), have been described in the normal appearing white matter and pre-demyelinating lesions of multiple sclerosis (MS) patients. As HIF-1α regulates the transcription of a wide set of genes involved in neuroprotection and neuroinflammation, HIF-1α expression may contribute to the pathogenesis of inflammatory demyelination. To test this hypothesis, we analyzed the effect of cell-specific genetic ablation or overexpression of HIF-1α on the onset and progression of experimental autoimmune encephalomyelitis (EAE), a mouse model for MS. HIF-1α was mainly expressed in astrocytes and microglia/macrophages in the mouse spinal cord at the peak of EAE. However, genetic ablation of HIF-1α in astrocytes and/or myeloid cells did not ameliorate clinical symptoms. Furthermore, conditional knock-out of Von Hippel Lindau, a negative regulator of HIF-1α stabilization, failed to exacerbate the clinical course of EAE. In accordance with clinical symptoms, genetic ablation or overexpression of HIF-1α did not change the extent of spinal cord inflammation and demyelination. Overall, our data indicate that despite dramatic upregulation of HIF-1α in astrocytes and myeloid cells in EAE, HIF-1α expression in these two cell types is not required for the development of inflammatory demyelination. Despite numerous reports indicating HIF-1α expression in glia, neurons, and inflammatory cells in the CNS of MS patients, the cell-specific contribution of HIF-1α to disease pathogenesis remains unclear. Here we show that although HIF-1α is dramatically upregulated in astrocytes and myeloid cells in EAE, cell-specific depletion of HIF-1α in these two cell types surprisingly does not affect the development of neuroinflammatory disease. Together with two recently published studies showing a role for oligodendrocyte-specific HIF-1α in myelination and T-cell-specific HIF-1α in EAE, our results demonstrate a tightly regulated cellular specificity for HIF-1α contribution in nervous system pathogenesis.
Tumor Enucleation for Renal Cell Carcinoma
Authors: Smith Zachary L. , Malkowicz S. Bruce 
Published: April 2015

The increased number of small renal masses (SRMs) detected annually has led to a rise in the use of nephron-sparing surgery (NSS). These techniques aim to preserve the largest amount of healthy renal tissue possible while maintaining the same oncologic outcomes as radical nephrectomy (RN). Additionally, partial nephrectomy (PN) has been linked to a lower risk of chronic kidney disease, cardiovascular morbidity, and mortality when compared to RN. There has been continual progress toward resecting less renal parenchyma. While the predominant surgical method of performing NSS is through traditional PN, simple enucleation (SE) of the tumor has increased in popularity over recent years. SE is a technique that aims to preserve the maximal amount of renal parenchyma possible by utilizing the renal tumor pseudocapsule to bluntly separate the lesion from its underlying parenchyma, offering the smallest possible margin of excised healthy renal tissue. Several studies have demonstrated the oncological safety of SE compared with PN in the treatment of SRMs, with lower overall incidence of positive surgical margins. Additionally, SE has been shown to have similar 5- and 10-year progression-free and cancer-specific survival as PN. We present a review of the literature and an argument for SE to be a routine consideration in the treatment of all renal tumors amenable to NSS.
O USO TERAPÊUTICO DA FLORA NA HISTÓRIA MUNDIAL
Authors: Rocha Francisco Angelo Gurgel da , Araújo Magnólia Fernandes Florêncio de , Costa Nilma Dias Leão , Silva Roberto Pereira da 
Published: March 2015

Este artigo descreve os principais fatos relacionados ao uso de espécies vegetais bioativas ao longo da história humana. São apresentados fatos e eventos que abrangem desde as comunidades pré-históricas, antiguidade (Índia, China, Grécia, Egito), idade Média e período das Grandes navegações. Em relação ao Brasil, são relatados os fatos mais marcantes na história pertinente ao uso da flora para finalidades terapêuticas, descrevendo-se os fatos mais marcantes, ocorridos desde o período Colonial até o início do Século XXI.
PLANTAS MEDICINAIS COMERCIALIZADAS EM MERCADOS PÚBLICOS NA REGIÃO METROPOLITANA DO RECIFE – PE: INFLUÊNCIA DO MODO DE CONSERVAÇÃO NA COMPOSIÇÃO QUÍMICA DO ÓLEO ESSENCIAL
Authors: Santos Andréia Gregório S , Dutra Kamilla A. , Dutra Maria Tereza Duarte , Silva Fabíola Soraia F.S.V.C.B. , Maranhão Cláudia Albuquerque , Navarro Daniela M.A.F. , Frutuoso Maria Núbia M.N.M.A. , Brandão Sofia Suely Ferreira 
Published: March 2015

O Grupo de Pesquisa em Educação e Interdisciplinaridade desenvolve ações de pesquisa, visando estudar o modelo de Gestão dos Mercados Públicos da Região Metropolitana do Recife. O objetivo é analisar seu funcionamento em relação aos aspectos de educação, ambiente, segurança alimentar e administração, para a elaboração de uma proposta de gestão ambiental. Neste contexto, no presente trabalho foi realizado o estudo químico de algumas plantas comercializadas no Mercado Público de Casa Amarela, para verificar a qualidade do produto oferecido ao consumidor pretendendo como resultado levar ao feirante orientações de correta conservação das plantas para que as mesmas mantenham seus princípios ativos. As plantas selecionadas foram R. officinalis, O. basilicum e C. murale, cujas folhas foram utilizadas frescas e secas naturalmente à temperatura ambiente. Foram realizadas extrações do óleo essencial das folhas e observou-se que, independentemente do óleo ser obtido de folhas frescas ou secas, manteve bons teores, semelhantes índices de refração e os mesmos componentes químicos majoritários. Assim, o processo de secagem foi vantajoso, representando uma importante contribuição ao processo de conservação destas plantas trazendo maior valor agregado ao produto, reduzindo as perdas pelo feirante e estabilizando a oferta ao consumidor.
Flow management and fish density regulate salmonid recruitment and adult size in tailwaters across western North America
Authors: Dibble Kimberly L. , Yackulic Charles B. , Kennedy Theodore A. , Budy Phaedra 
Published: March 2015

Rainbow and brown trout have been intentionally introduced into tailwaters downriver of dams globally and provide billions of dollars in economic benefits. At the same time, recruitment and maximum length of trout populations in tailwaters often fluctuate erratically, which negatively affects the value of fisheries. Large recruitment events may increase dispersal downriver where other fish species may be a priority (e.g., endangered species). There is an urgent need to understand the drivers of trout population dynamics in tailwaters, in particular the role of flow management. Here, we evaluate how flow, fish density, and other physical factors of the river influence recruitment and mean adult length in tailwaters across western North America, using data from 29 dams spanning 1–19 years. Rainbow trout recruitment was negatively correlated with high annual, summer, and spring flow and dam latitude, and positively correlated with high winter flow, subadult brown trout catch, and reservoir storage capacity. Brown trout recruitment was negatively correlated with high water velocity and daily fluctuations in flow (i.e., hydropeaking) and positively correlated with adult rainbow trout catch. Among these many drivers, rainbow trout recruitment was primarily correlated with high winter flow combined with low spring flow, whereas brown trout recruitment was most related to high water velocity.The mean lengths of adult rainbow and brown trout were influenced by similar flow and catch metrics. Length in both species was positively correlated with high annual flow but declined in tailwaters with high daily fluctuations in flow, high catch rates of conspecifics, and when large cohorts recruited to adult size. Whereas brown trout did not respond to the proportion of water allocated between seasons, rainbow trout length increased in rivers that released more water during winter than in spring. Rainbow trout length was primarily related to high catch rates of conspecifics, whereas brown trout length was mainly related to large cohorts recruiting to the adult size class. Species‐specific responses to flow management are likely attributable to differences in seasonal timing of key life history events such as spawning, egg hatching, and fry emergence.
Do PCAOB Inspection Reports Influence Corporate Executives' Perceptions of Audit Quality and the Likelihood of Switching Auditors?
Authors: Robertson Jesse C. , Stefaniak Chad M. , Houston Richard W. 
Published: April 2015

ABSTRACT
The PCAOB conducts inspections of public company auditors to improve audit quality and build investors' confidence in the quality of financial reporting (PCAOB 2010f). While there is some evidence that the inspection reports could be improving actual audit quality (e.g., Gramling et al. 2011; Carcello et al. 2011), their impact on perceptions of audit quality remains largely unexplored. We investigate the effects of inspection reports, which consistently disseminate negative information in the form of audit deficiencies (and in some cases, quality control criticisms) on perceived audit quality and potential auditor switching. We report the results of an experiment in which 90 corporate executives considered one of three response patterns that firms typically offer across multiple inspection reports: consistently provide concessions, consistently provide denials, or provide mixed responses that consist of both concessions and denials. We find that PCAOB inspection reports generally decrease perceived audit quality, regardless of response pattern, which, in turn, is generally associated with an increased likelihood that executives will consider switching auditors. We offer implications for audit policy and research, including the possibility that, while PCAOB inspections could be improving actual audit quality, the reports could be imposing costs by reducing perceived audit quality and, in turn, increasing the likelihood of auditor changes.
Data Availability: Contact the first author.
Expanding Sanitary Infrastructure and the Shaping of River History: River Vantaa (Finland) 1876-1982
Authors: Schönach Paula 
Published: March 2015

Abstract
This article investigates the environmental history of the River Vantaa, southern Finland, and its interaction with the spatial development of water-related infrastructures in an urbanising environment. The article argues that the overlapping use of the river as part of both waste water and drinking water infrastructures has shaped its ecological characteristics and consequently influenced communities' possibilities for using it. As a response to hydrological alterations, human activity focused on increasingly manipulating the river water and its flow. The study describes a gradual spatial expansion of the hydrological territory of the Finnish capital and a process of increasingly heavy engineered alterations in order to meet the water-related needs of communities in the region. This happened within the watershed and beyond, both above and under the surface. The study covers the time period from 1876 to 1982.
Simplified protocol for flow cytometry analysis of fluorescently labeled exosomes and microvesicles using dedicated flow cytometer
Authors: Pospichalova Vendula , Svoboda Jan , Dave Zankruti , Kotrbova Anna , Kaiser Karol , Klemova Dobromila , Ilkovics Ladislav , Hampl Ales , Crha Igor , Jandakova Eva , Minar Lubos , Weinberger Vit , Bryja Vitezslav 
Published: March 2015

Flow cytometry is a powerful method, which is widely used for high‐throughput quantitative and qualitative analysis of cells. However, its straightforward applicability for extracellular vesicles (EVs) and mainly exosomes is hampered by several challenges, reflecting mostly the small size of these vesicles (exosomes: ~80–200 nm, microvesicles: ~200–1,000 nm), their polydispersity, and low refractive index. The current best and most widely used protocol for beads‐free flow cytometry of exosomes uses ultracentrifugation (UC) coupled with floatation in sucrose gradient for their isolation, labeling with lipophilic dye PKH67 and antibodies, and an optimized version of commercial high‐end cytometer for analysis. However, this approach requires an experienced flow cytometer operator capable of manual hardware adjustments and calibration of the cytometer. Here, we provide a novel and fast approach for quantification and characterization of both exosomes and microvesicles isolated from cell culture media as well as from more complex human samples (ascites of ovarian cancer patients) suitable for multiuser labs by using a flow cytometer especially designed for small particles, which can be used without adjustments prior to data acquisition. EVs can be fluorescently labeled with protein‐(Carboxyfluoresceinsuccinimidyl ester, CFSE) and/or lipid‐ (FM) specific dyes, without the necessity of removing the unbound fluorescent dye by UC, which further facilitates and speeds up the characterization of microvesicles and exosomes using flow cytometry. In addition, double labeling with protein‐ and lipid‐specific dyes enables separation of EVs from common contaminants of EV preparations, such as protein aggregates or micelles formed by unbound lipophilic styryl dyes, thus not leading to overestimation of EV numbers. Moreover, our protocol is compatible with antibody labeling using fluorescently conjugated primary antibodies. The presented methodology opens the possibility for routine quantification and characterization of EVs from various sources. Finally, it has the potential to bring a desired level of control into routine experiments and non‐specialized labs, thanks to its simple bead‐based standardization.
Dans une pratique de soins psychiatriques à domicile : quel cadre thérapeutique pour permettre l’émergence d’un sujet libre ?
Authors: Denis Jennifer , Maisin Antoine , Giordano A. , Warichet J-M , Hendrick S. 
Published: April 2015

Depuis décembre 2011, l’état belge a soutenu la mise en œuvre d’équipes mobiles de psychiatrie financées à partir de la fermeture de lits hospitaliers. Des dispositifs de soins mobiles innovants ont été expérimentés notamment par l’équipe mobile Mons - Hainaut en Belgique. La mise en place d’une psychiatrie, hors des murs, bouleverse nos représentations coutumières de la psychiatrie institutionnelle. Elle nous amène à nous repositionner concernant le respect des libertés individuelles dans la mise en place de nouveaux cadres thérapeutiques à domicile. À travers cet article, nous déploierons et illustrerons le concept de triangulation thérapeutique ; outil médiateur dans notre pratique mobile de soins psychiatriques.
Le Belize, une société pluriculturelle sans politiques multiculturelles ?
Authors: Hoffmann Odile , Cunin Elisabeth 
Published: March 2015

Le Belize, ancienne colonie britannique en Amérique centrale, est généralement décrit en termes de diversité culturelle et de multiplicité des groupes ethniques qui le composent, par les observateurs comme par les administrateurs et gouvernants. Pourtant, depuis son indépendance récente (1981), le gouvernement bélizien n’a pas mis en place de politiques multiculturelles qui accorderaient un traitement différentiel à des individus en raison de leur appartenance ethnique ou raciale, comme on le constate dans les Amériques depuis les années 1980-90. Partant de ce constat, cet article se construit autour d’un double questionnement portant sur les modalités de la mise en place d’un projet national : comment le gouvernement bélizien a-t-il géré l’héritage du « divide and rule » colonial qui visait à segmenter la population ? Comment s’est-il adapté au « tournant multiculturel » des années 1980-90 et ses logiques de reconnaissance de la diversité ? Fondée sur deux champs d’application – les politiques culturelles et les politiques agraires-, l’analyse tend à montrer que la prise en compte de l’altérité par les politiques publiques s’inscrit dans la tradition coloniale britannique tout en intégrant les aspirations nées de la longue marche vers l’indépendance, et qu’elle suscite des formes spécifiques de gestion de la différence, au sens de politiques fondées sur des redistributions différentielles en fonction des appartenances collectives.
Arenc : le premier centre de rétention était clandestin
Authors: Naylor Ed 
Published: April 2015

De 1963 à 1975, un hangar situé à Arenc, dans la zone sécurisée du port de Marseille, est utilisé, dans le plus grand secret et en dehors de tout cadre juridique, pour l’hébergement d’étrangers en instance d’expulsion. 50 000 vont y transiter. Si la découverte de cette « prison clandestine » fait scandale, elle aboutit aussi à l’inscription de la rétention administrative dans l’arsenal législatif français.
La question du tact en psychanalyse
Authors: Leroy-Viémon Brigitte , Vivès Jean-Michel 
Published: April 2015

Freud, dans son texte sur l’analyse profane (1926a), alertait le lecteur sur le fait que lorsqu’on a trouvé une interprétation, il faut attendre le bon moment pour la communiquer au patient. A la question « à quoi reconnaît-on chaque fois le bon moment ? », il répondait que c’était une question de « Takt », traduit en français par le mot « flair ». Les auteurs de l’article s’emparent de cette question pour en approfondir la portée en clinique psychanalytique. S’appuyant sur les approches phénoménologique et psychanalytique, ils montrent que le tact, dans le cadre de la rencontre clinique, relève d’une dimension temporelle particulière créant de la profondeur dans l’instant. Ce faisant, il se présente comme la possibilité de mettre en résonance le réel humain pour le faire échoir au symbolique.
Productivité : quelles régions sont les moteurs économiques du Québec ?
Authors: Gagné Robert , Deslauriers Jonathan , Paré Jonathan , d’Amours Liette 
Published: March 2015

Il n’est de secret pour personne que le Québec accuse un sérieux retard en matière de productivité. Plusieurs recherches ont d’ailleurs été menées sur le sujet. Ce que l’on connaît toutefois moins, c’est le rôle joué par chacune des 17 régions administratives dans le développement économique de la province. Sur quelles régions le Québec peut-il compter pour accroître sa productivité ? Lesquelles en constituent le moteur économique et lesquelles sont à la remorque ? Voilà le type de questions auxquelles répond une récente étude réalisée par le Centre sur la productivité et la prospérité (CPP) de HEC Montréal ( cpp.hec.ca ) 1 .