bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibitor of cardiolipin biosynthesis‐related enzyme MoGep4 confers broad‐spectrum anti‐fungal activity
Authors: Sun Peng , Zhao Juan , Sha Gan , Zhou Yaru , Zhao Mengfei , Li Renjian , Kong Xiaojing , Sun Qiping , Li Yun , Li Ke , Bi Ruiqing , Yang Lei , Qin Ziting , Huang Wenzheng , Wang Yin , Gao Jie , Chen Guang , Zhang Haifeng , Adnan Muhammad , Yang Long , Zheng Lu , Chen Xiao‐Lin , Wang Guanghui , Ishikawa Toshiki , Li Qiang , Xu Jin‐Rong , Li Guotian 
Published: July 2024

AbstractPlant pathogens cause devastating diseases, leading to serious losses to agriculture. Mechanistic understanding of pathogenesis of plant pathogens lays the foundation for the development of fungicides for disease control. Mitophagy, a specific form of autophagy, is important for fungal virulence. The role of cardiolipin, mitochondrial signature phospholipid, in mitophagy and pathogenesis is largely unknown in plant pathogenic fungi. The functions of enzymes involved in cardiolipin biosynthesis and relevant inhibitors were assessed using a set of assays, including genetic deletion, plant infection, lipidomics, chemical‐protein interaction, chemical inhibition, and field trials. Our results showed that the cardiolipin biosynthesis‐related gene MoGEP4 of the rice blast fungus Magnaporthe oryzae regulates growth, conidiation, cardiolipin biosynthesis, and virulence. Mechanistically, MoGep4 regulated mitophagy and Mps1‐MAPK phosphorylation, which are required for virulence. Chemical alexidine dihydrochloride (AXD) inhibited the enzyme activity of MoGep4, cardiolipin biosynthesis and mitophagy. Importantly, AXD efficiently inhibited the growth of 10 plant pathogens and controlled rice blast and Fusarium head blight in the field. Our study demonstrated that MoGep4 regulates mitophagy, Mps1 phosphorylation and pathogenesis in M. oryzae. In addition, we found that the MoGep4 inhibitor, AXD, displays broad‐spectrum antifungal activity and is a promising candidate for fungicide development.
Effect of Alkaline Concentration and Temperature on Lignin Extraction from Sugarcane Bagasse
Authors: Chok Yong Xin , Zulkornain Alia Sabrina , Ya'akob Harisun , Mahmad Puzi Nur Syazwani , Wan Azelee Nur Izyan 
Published: July 2024

Sugarcane (Saccharum officinarum L.) is used in various applications, including the production of raw sugar, to be consumed, either directly as food or sugarcane juice. It is reported that 1 ton of sugarcane would generate nearly 280 kg of bagasse and they would be burnt up and leads to air pollution. In fact, sugarcane bagasse contains high lignin content, having Ultraviolet (UV) protection properties. The objective of this study is to determine the best concentration of sodium hydroxide and temperature to extract high yield of lignin for UV protection properties. In this project, sugarcane bagasse is collected from hawker stall in Taman Universiti, Skudai, Johor, Malaysia. The lignin composition in sugarcane bagasse was analyzed, giving 16.7± 4.8 wt% of sugarcane bagasse. Alkali extraction of sugarcane bagasse has been carried out by using different concentration of sodium hydroxide (NaOH) (2%, 4%, 6%, 8%, 10%) and temperatures (60 °C, 80 °C, 100 °C, 120 °C, 140 °C) in water bath at a fixed time, 1 hour. According to the experiment conducted, the best condition is 10% NaOH concentration, temperature of 100 °C for 1 hour, as the highest yield of lignin can be extracted, which is 55.8±1.92 wt%, with a purity of 28.1±10.75%. Ultraviolet protection properties of lignin had been analyzed by determining the Sun Protection Factor (SPF). It was done by blending different concentration of lignin into a base cream. The SPF achieved for 5% lignin-blended cream was 4.76 and for 10% lignin-blended cream was 5.78 respectively.
Towards a Conceptual Framework for Understanding the Protection of Farmers' Land Rights in China
Authors: Wang Xiangzhuo , Mohamed Yusof Nor Ashikin , Salleh Siti Hasliah 
Published: July 2024

Like in other parts of the world, land rights are crucial for f farmers in China too for they livelihoods and well- being. However, farmers often face various challenges in protecting their land rights, such as unclear property rights, inadequate legal protection, and insufficient compensation for land acquisition. This paper aims to establish a conceptual framework for understanding the protection of farmers' land rights in China thus addresses the farmer’ land rights problems in a better manner. The proposed framework which is built based on review of existing literature and an analysis of the current situation of farmers' land rights protection in China focuses on legal, institutional, and social dimensions. The legal dimension examines the legal basis for farmers' land rights, their executions and the effectiveness of the legal regulations in protecting these rights. The institutional dimension explores the roles and responsibilities of various government agencies and organizations in safeguarding farmers' land rights. The social dimension considers the impact of social factors, such as culture, tradition, and power relations, on the protection of farmers' land rights. When adopted the proposed framework could serve as a comprehensive guidance for the policy and decision makers of the government, farmers and other stakeholders to understand the protection of land rights.
Long-Term Estimation of Depositions on Heating Surface During Boiling of Long-Life Coolant
Authors: Shinozaki M. , Ito D. , Odaira N. , Ito K. , Saito Y. 
Published: July 2024

Abstract
Long-term boiling experiments with long-life coolant have been made with the aim to apply a boiling cooling technology to the next generation high exothermic electronic devices. The long-life coolant commonly used for cooling electronic devices is a mixture of ethylene glycol and water with multiple antirust inhibitors as additives, which may result in some depositions on the heat transfer surface during the boiling. In this study, the heat transfer surface made of copper was set vertically, and long-term experiments have been performed under the pool boiling. The deposition process was monitored for constant heating conditions. From experimental results, a distinct surface temperature change was observed under constant heat flux conditions. Just after keeping constant heat flux, the surface temperature increases with time, a certain time later decreases, and finally takes a stable value. During the temperature rise, the deposition adheres to the heat transfer surface in dots, which may cause an increase in thermal resistance between the wall surface and the working fluid. However, during the temperature drop, large growth of dot-like depositions on the heat transfer surface could alter wettability and surface roughness, enhancing boiling heat transfer. In addition, to investigate the characteristics of the depositions adhered to the heat transfer surface, a component analysis has been performed, which shows that the main substance was strontium hydroxyapatite, which might be formed by chemical reaction between components in the additives under the boiling.
Performance of Borated Centrifugally Tensioned Metastable Fluid Detector Versus Ludlum 42-49B, Fuji NSN3 Detectors for Fission Energy Spectrum Neutron Detection With the Source Within Lead/Concrete Shielded Configurations
Authors: Ota Yusuke , Taleyarkhan Rusi P. 
Published: July 2024

Abstract
This paper presents the results of neutron detection efficiency and dosimetry between a borated centrifugally tensioned metastable fluid detector (B-CTMFD) configured to detect thermal to fast neutrons versus two widely used neutron detection devices of similar form factors: moderated He-3 based Ludlum-42-49B neutron detector, and, the Fuji Electric's NSN3TM (NNSN3) pressurized nitrogen-methane filled neutron detector. The one-on-one performance comparisons were conducted using soft (Cf-252 fission) neutron spectrum isotope neutron sources positioned behind various levels of lead and concrete shielding ranging in thickness from 0 cm (unshielded) to up to 30 cm. The comparisons were conducted with Monte Carlo N-Particle transport code (MCNP) code simulations to account for three-dimensional effects and to relate the absolute detection rate with the dose rate—to derive the sensitivity factor (cpm/μSv/h). While the Ludlum 42-49B and NSN3 detectors operate at a fixed sensitivity setting, the centrifugally tensioned metastable fluid detector (CTMFD) can be (and was) operated to detect and separate the contributions from epithermal (0.02 eV–0.2 MeV) and from fast (&gt;0.2 MeV) neutrons at various sensitivity levels by varying the tensioned metastable negative pressure (Pneg) from 0.3 MPa to 0.7 MPa. The B-CTMFD (configured for detecting the full 0.02 eV to 12 MeV neutrons &gt;0.1 MeV neutron detection at Pneg = 0.7 MPa) offered relative sensitivity enhancements of up to ∼22× greater than Ludlum and over 5× greater than NSN3, over the 0-15 cm range of Pb shielding, and the 0–30 cm concrete shield thickness. The contribution from detecting down scattered neutrons increases with increased thickness, especially for concrete shielding. The B-CTMFD design overcomes the detection penalty (up to 60% depending on shielding type and thickness) inherent in the nonborated centrifugally tensioned metastable fluid detector (NB-CTMFD), designed only to detect fast-energy neutrons—as described in the companion (Part-1) paper. However, unlike the NB-CTMFD, which used 100% decafluorapentane (DFP) (C5H2F10), the B-CTMFD requires the use of an azeotropic mixture of DFP, methanol, and tri-methyl borate (TMB—C3H9BO3, using natural boron) in 80:4:16 proportion. The B-CTMFD was about 6 times more sensitive than NB-CTMFD under the most heavily shielded condition and, taken together, also offered 2-energy bin neutron spectroscopic enablement, together with 22-5× higher absolute efficiency and relative sensitivity compared with the nonspectroscopic Ludlum (He-3) and NSN3 (methane-nitrogen) based detectors. From an intrinsic efficiency standpoint, the B-CTMFD operating at Pneg = 0.7 MPa state demonstrated ∼103× higher intrinsic efficiency over Ludlum 42-49B and NSN3 detectors, respectively.
Trends in antidiabetes medication use among hospitalised patients with type 2 diabetes: a retrospective single-centre cohort study
Authors: Meier Nicole , Laager Rahel , Gregoriano Claudia , Schütz Philipp , Mueller Beat , Struja Tristan , Kutz Alexander 
Published: July 2024

Objectives
Novel antidiabetes medications with proven cardiovascular or renal benefit, such as sodium-glucose cotransporter-2 inhibitors (SGLT-2i) and glucagon-like peptide 1 receptor agonists (GLP-1 RA), have been introduced to the market. This study explored the 4-year trends of antidiabetes medication use among medical hospitalisations with type 2 diabetes (T2D).

Design
Retrospective cohort study.

Setting
Tertiary care hospital in Switzerland.

Participants
4695 adult hospitalisations with T2D and prevalent or incident use of one of the following antidiabetes medications (metformin, dipeptidyl peptidase-4 inhibitors (DPP-4i), sulfonylureas, GLP-1 RA, SGLT-2i, short-acting insulin or long-acting insulin), identified using electronic health record data. Quarterly trends in use of antidiabetes medications were plotted overall and stratified by cardiovascular disease (CVD) and chronic kidney disease (CKD).

Results
We observed a stable trend in the proportion of hospitalisations with T2D who received any antidiabetes medication (from 77.6% during 2019 to 78% in 2022; p for trend=0.97). In prevalent users, the largest increase in use was found for SGLT-2i (from 7.4% in 2019 to 21.8% in 2022; p for trend <0.01), the strongest decrease was observed for sulfonylureas (from 11.4% in 2019 to 7.2% in 2022; p for trend <0.01). Among incident users, SGLT-2i were the most frequently newly prescribed antidiabetes medication with an increase from 26% in 2019 to 56.1% in 2022 (p for trend <0.01). Between hospital admission and discharge, SGLT-2i also accounted for the largest increase in prescriptions (+5.1%; p<0.01).

Conclusions
These real-world data from 2019 to 2022 demonstrate a significant shift in antidiabetes medications within the in-hospital setting, with decreased use of sulfonylureas and increased prescriptions of SGLT-2i, especially in hospitalisations with CVD or CKD. This trend aligns with international guidelines and indicates swift adaptation by healthcare providers, signalling a move towards more effective diabetes management.
Critical appraisal and assessment of bias among studies evaluating risk prediction models for in-hospital and 30-day mortality after percutaneous coronary intervention: a systematic review
Authors: Shi Yankai , Zhu Chen , Qi Wenhao , Cao Shihua , Chen Xiaomin , Xu Dongping , Wang Cheng 
Published: July 2024

ObjectiveWe systematically assessed prediction models for the risk of in-hospital and 30-day mortality in post-percutaneous coronary intervention (PCI) patients.DesignSystematic review and narrative synthesis.Data sourcesSearched PubMed, Web of Science, Embase, Cochrane Library, CINAHL, CNKI, Wanfang Database, VIP Database and SinoMed for literature up to 31 August 2023.Eligibility criteriaThe included literature consists of studies in Chinese or English involving PCI patients aged ≥18 years. These studies aim to develop risk prediction models and include designs such as cohort studies, case–control studies, cross-sectional studies or randomised controlled trials. Each prediction model must contain at least two predictors. Exclusion criteria encompass models that include outcomes other than death post-PCI, literature lacking essential details on study design, model construction and statistical analysis, models based on virtual datasets, and publications such as conference abstracts, grey literature, informal publications, duplicate publications, dissertations, reviews or case reports. We also exclude studies focusing on the localisation applicability of the model or comparative effectiveness.Data extraction and synthesisTwo independent teams of researchers developed standardised data extraction forms based on CHecklist for critical Appraisal and data extraction for systematic Reviews of prediction Modelling Studies to extract and cross-verify data. They used Prediction model Risk Of Bias Assessment Tool (PROBAST) to assess the risk of bias and applicability of the model development or validation studies included in this review.ResultsThis review included 28 studies with 38 prediction models, showing area under the curve values ranging from 0.81 to 0.987. One study had an unclear risk of bias, while 27 studies had a high risk of bias, primarily in the area of statistical analysis. The models constructed in 25 studies lacked clinical applicability, with 21 of these studies including intraoperative or postoperative predictors.ConclusionThe development of in-hospital and 30-day mortality prediction models for post-PCI patients is in its early stages. Emphasising clinical applicability and predictive stability is vital. Future research should follow PROBAST’s low risk-of-bias guidelines, prioritising external validation for existing models to ensure reliable and widely applicable clinical predictions.PROSPERO registration numberCRD42023477272.
Effect of epidural anesthesia on the optic nerve sheath diameter in patients with pre-eclampsia: a prospective observational study
Authors: Park Sun-Kyung , Kim Hansol , Kim Youngwon , Jang Young-Eun , Kim Jin-Tae 
Published: July 2024

IntroductionOptic nerve sheath diameter (ONSD) reflects intracranial pressure and is increased in pre-eclampsia. Administrating a significant volume of epidural solution into the epidural space can potentially increase ONSD. We investigated the impact of epidural local anesthetic injection on ONSD in patients with pre-eclampsia.MethodsPatients with pre-eclampsia (n=11) and normotensive pregnant women (n=11) received de novo epidural anesthesia for cesarean delivery. We administered 21 mL of an epidural solution containing 2% lidocaine and 50 μg fentanyl into the lumbar epidural space in incremental doses. ONSD was measured at baseline, 3, 10, and 20 min after completing the epidural injection, after delivery, and at the end of surgery. Primary outcome was the change in ONSD from baseline to 3 min after epidural injection in patients with pre-eclampsia and normotensive pregnant women. Serial changes in the ONSD were analyzed using a linear mixed model.ResultsAt baseline and 3 min after epidural drug injection, ONSD was significantly larger in patients with pre-eclampsia than in normotensive mothers (5.7 vs 4.1 mm, p=0.001 and 5.4 vs 4.1 mm, p<0.001, respectively). However, there were no significant changes in ONSD at 3 min after injection from baseline in either group (p>0.999). Linear mixed model demonstrated that ONSD did not change after epidural anesthesia in either group (p=0.279 and p=0.347, respectively).ConclusionsDespite a higher baseline ONSD in pre-eclampsia, epidural anesthesia did not further increase ONSD. Our findings indicate that epidural anesthesia can be safely administered in patients with pre-eclampsia at risk of increased intracranial pressure, without other intracranial pathology.Trial registration numberNCT04095832.
Impact of English proficiency on use of Glasgow Coma Scale in geriatric patients with traumatic brain injury
Authors: Layrisse-Landaeta Veronica , Dincheva Gabriela R , Khedr Shahenda , Geng Andrew , Schombs Michele , Maisha Kazi , Chao Steven Y , Chen Chun-Cheng 
Published: July 2024

BackgroundThe relationship between English proficiency (EP), Glasgow Coma Scale (GCS) and traumatic brain injury (TBI) is not well characterized. We aimed to understand the impact of limited English proficiency (LEP) on the evaluation and outcomes of TBI.MethodsRetrospective comparative study in a single institution of patients aged ⪰65 who presented to the emergency department after a fall with head strike between January 2018 and December 2021. TBI was defined as documented loss of consciousness or intracranial hemorrhage (ICH). Relationships between EP, GCS, and TBI were analyzed with multivariable and propensity score-matched models.ResultsOf the 2905 included, 1233 (42%) had LEP. Most LEP patients were Asian (60%) while the majority of EP patients were non-Hispanic Caucasians (72%). In a univariate analysis, LEP had higher incidence of decreased GCS and was strongly correlated with risk of TBI (OR 1.47, CI 1.26 to 1.71). After adjusting for multiple covariates including race, LEP did not have a significantly increased risk for GCS score <13 (OR 1.66, CI 0.99 to 2.76) or increased risk of TBI. In the matched analysis, LEP had a small but significantly higher risk of GCS score <13 (OR 1.03, CI 1.02 to 1.05) without an increased risk in TBI. Decreased GCS remained strongly correlated with presence of ICH in LEP patients in the adjusted model (OR 1.39, CI 1.30 to 1.50).ConclusionsLEP correlated with lower GCS in geriatric patients with TBI. This association weakened after adjusting for factors like race, suggesting racial disparities may have more influence than language differences. Moreover, GCS remained effective for predicting ICH in LEP individuals, highlighting its value with suitable translation resources.Level of evidenceThis is a Level III evidence restrospective comparative study.
N-Myristoytransferase Inhibition Causes Mitochondrial Iron Overload and Parthanatos in TIM17A-Dependent Aggressive Lung Carcinoma
Authors: Geroyska Sofia , Mejia Isabel , Chan Alfred A. , Navarrete Marian , Pandey Vijaya , Kharpatin Samuel , Noguti Juliana , Wang Feng , Srole Daniel , Chou Tsui-Fen , Wohlschlegel James , Nemeth Elizabeta , Damoiseaux Robert , Shackelford David B. , Lee Delphine J. , Díaz Begoña 
Published: July 2024

Abstract

Myristoylation is a type of protein acylation by which the fatty acid myristate is added to the N-terminus of target proteins, a process mediated by N-myristoyltransferases (NMT). Myristoylation is emerging as a promising cancer therapeutic target; however, the molecular determinants of sensitivity to NMT inhibition or the mechanism by which it induces cancer cell death are not completely understood. We report that NMTs are a novel therapeutic target in lung carcinoma cells with LKB1 and/or KEAP1 mutations in a KRAS-mutant background. Inhibition of myristoylation decreases cell viability in vitro and tumor growth in vivo. Inhibition of myristoylation causes mitochondrial ferrous iron overload, oxidative stress, elevated protein poly (ADP)-ribosylation, and death by parthanatos. Furthermore, NMT inhibitors sensitized lung carcinoma cells to platinum-based chemotherapy. Unexpectedly, the mitochondrial transporter translocase of inner mitochondrial membrane 17 homolog A (TIM17A) is a critical target of myristoylation inhibitors in these cells. TIM17A silencing recapitulated the effects of NMT inhibition at inducing mitochondrial ferrous iron overload and parthanatos. Furthermore, sensitivity of lung carcinoma cells to myristoylation inhibition correlated with their dependency on TIM17A. This study reveals the unexpected connection between protein myristoylation, the mitochondrial import machinery, and iron homeostasis. It also uncovers myristoylation inhibitors as novel inducers of parthanatos in cancer, and the novel axis NMT-TIM17A as a potential therapeutic target in highly aggressive lung carcinomas.

Significance:
KRAS-mutant lung carcinomas with LKB1 and/or KEAP1 co-mutations have intrinsic therapeutic resistance. We show that these tumors are sensitive to NMT inhibitors, which slow tumor growth in vivo and sensitize cells to platinum-based chemotherapy in vitro. Inhibition of myristoylation causes death by parthanatos and thus has the potential to kill apoptosis and ferroptosis-resistant cancer cells. Our findings warrant investigation of NMT as a therapeutic target in highly aggressive lung carcinomas.