bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental DNA metabarcoding on aquatic insects: Comparing the primer sets of MtInsects‐16S based on the mtDNA 16S and general marker based on the mtDNA COI region
Authors: Takenaka Masaki , Hasebe Yuta , Yano Koki , Okamoto Seiya , Tojo Koji , Seki Masashi , Sekiguchi Shun , Jitsumasa Takeshi , Morohashi Nobuyuki , Handa Yoshihiro , Sakaba Tomoka 
Published: July 2024

AbstractLong‐term biodiversity monitoring is necessary for conservation and management. In such circumstances, environmental DNA (eDNA) surveys can enable easy and effective biomonitoring of aquatic insects. However, previous studies of aquatic insects based on the mtDNA COI region have revealed incomplete taxonomic coverage, and frequent amplification of nontarget taxa (e.g., algae and diatoms). Additionally, it has been indicated that there are few reference sequences registered in databases for metabarcoding analysis of insects. Therefore, we developed a new primer set, MtInsects‐16S, for eDNA analyses of insects based on the mtDNA 16S rRNA region in a previous study. To address the insufficient database records of insect DNA sequences, we also constructed a comprehensive reference database of aquatic insects that occur in Kanagawa Prefecture, Japan. We conducted eDNA analyses at six sites in the two river systems (the Sagami‐gawa River and Sakawa‐gawa River systems), with three replicates per site, based on both the mtDNA COI and the 16S rRNA regions. These results were compared physical capture surveys at the same sites to examine the detection capability of eDNA for Insecta with using a well‐established database. Among the list of species which were collected by physical capture surveys, 74.9% were detected by MtInsects‐16S, whereas 40.1% were detected by the primer set of the mtDNA COI region, and also 80.0% were detected by the both primer sets. This study demonstrated that the application of eDNA analyses using the MtInsects‐16S primer set can be conducted with accuracy and reliability, provided that the reference DNA database is improved. The MtInsects‐16S region can be considered indispensable in eDNA analysis for aquatic insects.
Micronutrient interactions: Magnesium and its synergies in maternal–fetal health
Authors: Shukla Vani , Parvez Sidrah , Fatima Ghizal , Singh Shikha , Magomedova Aminat , Batiha Gaber El‐Saber , Alexiou Athanasios , Papadakis Marios , Welson Nermeen N. , Hadi Najah 
Published: July 2024

AbstractMagnesium is an essential nutrient for various physiological processes and becomes even more vital during pregnancy, contributing to muscle relaxation, bone development, electrolyte balance, and blood pressure regulation. Despite the fact that the dietary sources of magnesium are diversified, it is still challenging to obtain it in sufficient quantities during pregnancy. We have elucidated its interactions and its specific impact on maternal–fetal health in different research publications. Magnesium also interacts synergistically with other micronutrients like calcium, vitamin D, potassium, zinc, iron, and vitamin B6, emphasizing its significance in promoting optimal outcomes for both the mother and the fetus. Inadequate magnesium intake during pregnancy has been linked to complications like gestational diabetes, preeclampsia, preterm birth, and low birth weight. Therefore, there should be an emphasis on the importance of maintaining adequate magnesium levels through a balanced diet and supplementation. This research therefore studies the complex mechanisms of micronutrient interactions and their specific impacts on maternal–fetal health to enhance prenatal care practices.
Nasopharyngeal cancer mortality in disaggregated Asian and non‐Asian Americans
Authors: Hung George A. , Vohra Sanah , Kim Gina , Jamal Armaan , Srinivasan Malathi , Huang Robert J. , Kim Gloria , Palaniappan Latha , Colevas A. Dimitrios 
Published: July 2024

AbstractBackgroundNasopharyngeal carcinoma (NPC) mortality varies based on multiple risk factors. While NPC mortality is higher in Asia, little is known about Asian subgroups in the United States (US).MethodsUsing the 2005–2020 National Vital Statistics System, we examined NPC mortality by age, race (non‐Hispanic black, Hispanic white (HW), non‐Hispanic white (NHW), Chinese, Filipino, Asian Indian, Japanese, Korean, Vietnamese), sex, and nativity (Untied States or foreign‐born).ResultsUpon disaggregation, Chinese (1.96 [CI: 1.78–2.16]), Filipino (0.68 [0.68–1.11]), and Vietnamese Americans (0.68 [0.52–1.10]) had the top age‐adjusted mortality rates (AAMR per 100 000 person‐years). Foreign‐born Chinese, Vietnamese, Filipinos, Asian Indians, and NHW had higher AAMRs compared to US‐born persons. All male groups had higher AAMR compared to females. Stratifying for race, nativity, and sex, foreign‐born Chinese males (4.09 [3.79–4.40]) had the highest AAMR.ConclusionThese findings demonstrate the importance of disaggregating NPC mortality data by Asian subgroups, providing valuable insights for targeted public health interventions in the United States.
Supporting health systems and environment in the Democratic Republic of Congo: A call for action
Authors: Mufungizi Innocent , Okon Inibehe , Nkundakozera Mwangaza , Akilimali Aymar 
Published: July 2024

AbstractBackgroundDeveloping nations have to overcome a number of obstacles to fulfill the Sustainable Development Goals. The Democratic Republic of Congo is one of the five poorest nations in the world and faces several challenges in combating problems related to poverty, health, and sanitation while linking the environment to anthropogenic activities.MethodsThis study analyzes anthropogenic activities and their impact on the environment while providing access to the public health of the Congolese population based on the objectives of sustainable development. Thirty‐five articles were selected for further analysis as well as relative data.ResultsIn 2022, 21 million cases of malaria were recorded by the national malaria control program, with 13,000 cases of death. The Democratic Republic of Congo has the highest typhoid incidence, with 315 cases per 100,000 people. A number of 31,342 cases of cholera were reported in 2023, according to multiple reports, with 230 deaths, mainly affecting children. In the same year, a triple epidemic of typhoid, shigellosis, and cholera was identified, with 2389 cases and 52 deaths. These observations cause a health emergency, which can be alleviated and resolved by the establishment of an adequate sanitation system. Waste can be recycled and returned to usable raw materials.ConclusionFinally, it will be necessary to establish a water safety management plan to combat all diseases linked to the consumption of nonpotable water and improve national coverage on the treatment of recent cases to reduce and at best avoid observed cases of death.
Rat nasal mucosa‐derived ectodermal mesenchymal stem cells: A new therapeutic option for chronic rhinosinusitis
Authors: Li Liujin , Liu ZhaoHui , Zhang ChunLin , Long YiLin , Yang TianWen 
Published: July 2024

AbstractObjectiveTo investigate the effect of nasal mucosa‐derived ectodermal mesenchymal stem cells (NM‐EMSCs) on the inflammatory state of rats with chronic rhinosinusitis (CRS) and the underlying therapeutic mechanism.MethodsNM‐EMSCs were isolated and extracted to construct a rat model of CRS. Fifteen Sprague‒Dawley (SD) rats were randomly divided into three groups: CK + NS group rats were injected locally with saline in the nasal mucosa; CRS + NS group rats were injected locally with saline in the nasal mucosa; and CRS + EMSCs group rats were injected locally with NM‐EMSCs in the nasal mucosa. One rat from the CRS + EMSCs group was randomly euthanized at 2, 4, and 6 days after injection, and the nasal mucosa tissues were collected for HE staining, Masson's trichrome staining, and periodic acid–Schiff staining.ResultsNM‐EMSCs specifically expressing CD73, CD105, and CD90 were successfully isolated from the nasal mucosa of rats and were able to differentiate into adipocytes, osteoblasts, and chondrocytes. After saline and NM‐EMSC injection, compared with those in the blank control CK + NS group, the nasal mucosa in the CRS + NS and CRS + EMSC groups exhibited obvious thickening, a large amount of inflammatory cell infiltration, and increased collagen and mucin distribution. Four days post‐NM‐EMSC injection, the thickening of the nasal mucosa in the CRS group was gradually alleviated, the inflammatory cell infiltration gradually decreased, and the distribution of collagen and mucin and the collagen‐positive area gradually decreased. Moreover, only a small number of inflammatory cells were visible, and the distribution of mucins was limited to 6 days post‐NM‐EMSC injection.ConclusionNM‐EMSCs effectively attenuated inflammation in the nasal mucosa of CRS model rats.
Development of hyaluronic acid hydrogel containing prednisolone‐encapsulated nonphospholipid liposomes for the treatment of rheumatoid arthritis
Authors: Tsai Wei‐Bor , Chen Chin‐Ju 
Published: July 2024

AbstractRheumatoid arthritis (RA) requires therapeutic approaches that alleviate symptoms and inhibit the progression of joint damage. Glucocorticoids (GCs) have been a cornerstone of RA treatment, yet their use is often limited by side effects. Recent advancements suggest that liposome‐based delivery systems can improve GC biodistribution, minimizing toxicity. This study introduces an innovative tool for RA treatment using prednisone‐encapsulated nonphospholipid liposomes (NPLs) in combination with a hyaluronic acid (HA) hydrogel. Our methodology involved incorporating prednisone (PR) with palmitic acid and cholesterol to formulate stable NPLs using a thin‐film hydration technique. The synthesized PR‐NPLs, characterized by a mean size of 150 nm, demonstrated uniform distribution and higher drug encapsulation in comparison with conventional phospholipid liposomes. In vitro assays revealed that PR‐NPL markedly reduced inflammatory responses in macrophages. Additionally, we successfully incorporated PR‐NPL into an HA hydrogel, employing a photoinitiated cross‐linking process. This novel composite offered modulable PR release, governed by the degree of hydrogel cross‐linking. The developed system presents a promising advancement in RA management, especially suited for intraarticular injections. It potentially enables targeted, controlled drug release with a reduced risk of side effects, signifying a significant improvement over existing RA therapies.
Synthesis of 2‐aminothiazoles containing 3‐hydroxypyran‐4‐one fragment based on condensation of substituted α‐arylaminoketones with thiourea
Authors: Komogortsev Andrey N. , Melekhina Valeriya G. , Milyutin Constantine V. , Lichitsky Boris V. 
Published: July 2024

AbstractCondensation of α‐arylaminoketones bearing 3‐hydroxypyran‐4‐one fragment with thiourea was studied. Based on considered investigation, the method for the synthesis of terarylenes with 2‐aminothiazole bridge was elaborated. The presented process is the first example of construction of thiazole core starting from α‐aminoketone precursor. The advantages of developed approach are easily available starting materials and convenient isolation of target products avoiding chromatographic purification. The structure of one of prepared products was confirmed by x‐ray analysis. The synthetic utility of obtained 2‐aminothiazoles with allomaltol substituent was demonstrated by reaction at amino and hydroxyl groups.
Tongue‐tie in the Neonatal Intensive Care Unit Compared to Healthy Newborns
Authors: McKenna Margo K. , Rosen‐Carole Casey , Burtner Michele , Wilson John L. , Greenman Suzie , Shah Shalini , Allen Paul 
Published: July 2024

ObjectiveWe sought to characterize the prevalence of ankyloglossia in our neonatal intensive care unit (NICU) population and to determine characteristics of this cohort compared to infants in the birth center (BC).MethodsProspective data were collected using a standardized flow sheet. Breastfeeding infants undergoing evaluation for tongue‐tie in the BC and NICU were included. Coryllos type, tip to frenulum length, tongue function, frequency of frenotomy, and breastfeeding outcomes were compared.ResultsOf 20,879 infants birthed at or admitted to the institution during the study period, there were fewer patients diagnosed with ankyloglossia in the BC compared to the NICU (3.3% BC vs. 5.4% NICU, p < 0.01). Of these, 163 underwent frenotomy: 86 in the BC and 77 in the NICU. For those undergoing frenotomy, gestational age (39.1 ± 1.3 BC, 34.4 ± 4.4 NICU, p < 0.01) and age at time of procedure (3.2 days BC, 29.2 NICU, p < 0.01) were the only demographic factors significantly different between the groups. There was no difference in Coryllos type or function score. In a subset of NICU infants with multiple assessments over time, function scores after frenotomy were significantly improved compared to pre‐frenotomy (p < 0.01).ConclusionStandard assessment tools appear to be appropriate for use in infants in the NICU, despite the higher rates of prematurity, low birth weights, and increased comorbidities. Assessment and intervention for tongue‐tie can be one critical intervention to move these patients closer to oral feeding and discharge to home.Level of Evidence3 Laryngoscope, 134:5152–5159, 2024
The Conjugated/Non‐Conjugated Linked Dimer Acceptors Enable Efficient and Stable Flexible Organic Solar Cells
Authors: Yin Haoran , Xie Gang , Wu Tuhong , Liu Siqi , Chen Dong , Chen Yiwang 
Published: July 2024

AbstractThe fabrication of the flexible devices with excellent photovoltaic performance and stability is critical for the commercialization of organic solar cells (OSCs). Herein, the conjugated dimer acceptor DY‐TVCl and the non‐conjugated dimer acceptor DY‐3T based on the monomer MY‐BO are synthesized to regulate the molecular glass transition temperatures (Tg) for improving the morphology stability of active layer films. And the crack onset strain values for the blend films based on dimer acceptors are superior than that of small molecule, which are beneficial for the preparation of flexible devices. Accordingly, the binary device based on PM6:DY‐TVCl achieves a maximum power conversion efficiency (PCE) of 18.01%. Meanwhile, the extrapolated T80 (time to reach 80% of initial PCE) lifetimes of the PM6:DY‐TVCl‐based device and PM6:DY‐3T‐based device are 3091 and 2227 h under 1‐sun illumination, respectively, which are better than that of the PM6:MY‐BO‐based device (809 h). Furthermore, the flexible devices based on DY‐TVCl and DY‐3T exhibit the efficiencies of 15.23% and 14.34%, respectively. This work affords a valid approach to improve the stability and mechanical robustness of OSCs, as well as ensuring the reproducibility of organic semiconductors during mass production.
A Food Odorant, α‐Ionone, Inhibits Skin Cancer Tumorigenesis by Activation of OR10A6
Authors: Yoon Ye Eun , Jung Young Jae , Lee Sung‐Joon 
Published: July 2024

ScopeThis study aims to investigate the anticancer properties of α‐ionone in squamous cell carcinoma (SCC).Methods and resultsThe expression of OR10A6 together with olfactory receptor signaling components is demonstrated in A431 human SCC cells via RT‐PCR and qRT‐PCR analysis. OR10A6 activation in A431 cells using the ligand α‐ionone inhibits proliferation and migration but induces apoptosis which is confirmed by proliferation assay, colony formation, and western blotting. The mechanism involves the core proteins of the Hippo pathway, where the phosphorylation of large tumor suppressor kinase (LATS), yes‐associated protein (YAP), and transcriptional coactivator with PDZ‐binding motif (TAZ) is confirmed by western blotting. However, the anticancer effects of α‐ionone are abrogated in A431 cells with OR10A6 gene knockdown. In A431 xenograft mouse model, the injection of α‐ionone suppresses tumor growth, induces apoptosis, and increases phosphorylation of the LATS‐YAP‐TAZ signaling axis in the Hippo pathway. None of these effects are observed in xenografted tumors with OR10A6 gene knockdown.ConclusionThese findings collectively demonstrate that activation of ectopic OR OR10A6 by α‐ionone in SCC cells stimulates the Hippo pathway and suppresses tumorigenesis both in vitro and in vivo, suggesting a novel therapeutic candidate for the treatment of SCC.
Fructooligosaccharides Ameliorate Renal Injury and Dysfunction Through the Modulation of Gut Dysbiosis, Inhibition of Renal Inflammation, Oxidative Stress, Fibrosis, and Improve Organic Anion Transporter 3 Function in an Obese Rat Model
Authors: Pengrattanachot Nattavadee , Thongnak Laongdao , Promsan Sasivimon , Phengpol Nichakorn , Sutthasupha Prempree , Tocharus Jiraporn , Lungkaphin Anusorn 
Published: July 2024

ScopeHigh‐fat diet (HFD) consumption causes obesity and gut dysbiosis which induces kidney injury. It has been reported that prebiotics improve gut dysbiosis and insulin sensitivity and decelerate the progression of kidney disease. This study investigates the impact of fructooligosaccharides (FOS) on renoprotection and the prevention of gut dysbiosis and intestinal barrier injury in obese rats.Methods and resultsWistar rats are treated with HFD for 16 weeks. Then, the HFD fed rats (HF) are given FOS 1 g day−1 (HFFOS1), 2 g day−1 (HFFOS2), or metformin 30 mg kg−1 day−1 (HFMET), by intragastric feeding for 8 weeks. Blood, urine, feces, kidney, and intestine are collected to determine the metabolic changes, gut dysbiosis, and the expression of proteins involved in kidney and intestinal injury. FOS can attenuate insulin resistance and hypercholesterolemia concomitant with the inhibition of renal inflammation, oxidative stress, fibrosis, and apoptosis, which are related to the deceleration of the overexpression of renal Toll‐like receptor 4 (TLR4) and NADPH oxidase (NOX4). Moreover, FOS shows a greater efficacy than metformin in the reduction of the intestinal injury and loss of tight junction proteins induced by HFD.ConclusionFOS may be used as a supplement for therapeutic purposes in an obese condition to improve intestinal integrity and prevent renal complications.
The lingering safety menace: A 10‐year review of enteral misconnection adverse events and narrative review
Authors: Ethington Stacie , Volpe Amanda , Guenter Peggi , Simmons Debora 
Published: July 2024

AbstractIn 2011, “Tubing Misconnections: Normalization of Deviance” reported >100 cases of enteral tubing misconnections leading to patient harm. Despite development of safer enteral device connectors, 96 new cases of enteral misconnections have been published since 2011. Publication and safety databases were searched for reports from 2011 to 2023. Reported misconnections lead to death in 4% of the cases and survival with harm were reported in 69% of cases. Reported misconnections occurred more often in infants and children than in adults. This article outlines why these misconnections happen, the history of the issue and development of safer connector standards, the safety threats and recommendations associated with the new cases, current conversion rates, and process steps, education, and resources for the conversion to safer connectors for enteral nutrition devices.
Histone demethylase PHF8 promotes prostate cancer metastasis via the E2F1–SNAI1 axis
Authors: Wang Ze , Tang Peng , Xiao Haiyang , Peng Song , Chen Jian , Wang Yapeng , Xu Jing , Yan Qian , Zhang Junying , Deng Jie , Ma Qiang , Zhu Hailin , Luo Weiming , Zhang Dianzheng , Wang Luofu , Qin Jun , Lan Weihua , Jiang Jun , Liu Qiuli 
Published: July 2024

AbstractMetastasis is the primary culprit behind cancer‐related fatalities in multiple cancer types, including prostate cancer. Despite great advances, the precise mechanisms underlying prostate cancer metastasis are far from complete. By using a transgenic mouse prostate cancer model (TRAMP) with and without Phf8 knockout, we have identified a crucial role of PHF8 in prostate cancer metastasis. By complexing with E2F1, PHF8 transcriptionally upregulates SNAI1 in a demethylation‐dependent manner. The upregulated SNAI1 subsequently enhances epithelial‐to‐mesenchymal transition (EMT) and metastasis. Given the role of the abnormally activated PHF8/E2F1‐SNAI1 axis in prostate cancer metastasis and poor prognosis, the levels of PHF8 or the activity of this axis could serve as biomarkers for prostate cancer metastasis. Moreover, targeting this axis could become a potential therapeutic strategy for prostate cancer treatment. © 2024 The Pathological Society of Great Britain and Ireland.
Functional ex vivoDNA fibre assay to measure replication dynamics in breast cancer tissue
Authors: Chen Mengting , van den Tempel Nathalie , Bhattacharya Arkajyoti , Yu Shibo , Rutgers Bea , Fehrmann Rudolf SN , de Haas Sander , van der Vegt Bert , van Vugt Marcel ATM 
Published: July 2024

AbstractReplication stress (RS) is a key trait of cancer cells, and a potential actionable target in cancer treatment. Accurate methods to measure RS in tumour samples are currently lacking. DNA fibre analysis has been used as a common technique to measure RS in cell lines. Here, we investigated DNA fibre analysis on fresh breast cancer specimens and correlated DNA replication kinetics to known RS markers and genomic alterations. Fresh, treatment‐naïve primary breast cancer samples (n = 74) were subjected to ex vivo DNA fibre analysis to measure DNA replication kinetics. Tumour cell proliferation was confirmed by EdU incorporation and cytokeratin AE1/AE3 (CK) staining. The RS markers phospho‐S33‐RPA and γH2AX and the RS‐inducing proto‐oncogenes Cyclin E1 and c‐Myc were analysed by immunohistochemistry. Copy number variations (CNVs) were assessed from genome‐wide single nucleotide polymorphism (SNP) arrays. We found that the majority of proliferating (EdU‐positive) cells in each sample were CK‐positive and therefore considered to be tumour cells. DNA fibre lengths varied largely in most tumour samples. The median DNA fibre length showed a significant inverse correlation with pRPA expression (r = −0.29, p = 0.033) but was not correlated with Cyclin E1 or c‐Myc expression and global CNVs in this study. Nuclear Cyclin E1 expression showed a positive correlation with pRPA levels (r = 0.481, p < 0.0001), while cytoplasmic Cyclin E1 expression exhibited an inverse association with pRPA expression (r = −0.353, p = 0.002) and a positive association with global CNVs (r = 0.318, p = 0.016). In conclusion, DNA fibre analysis performed with fresh primary breast cancer samples is feasible. Fibre lengths were associated with pRPA expression. Cyclin E1 expression was associated with pRPA and the percentage of CNVs. © 2024 The Author(s). The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.