bluuebunny/crossref_metadata_2025_split huggingface dataset:
Non‐Invasive Quality Control of Organoid Cultures Using Mesofluidic CSTR Bioreactors and High‐Content Imaging
Authors: Charles Seleipiri , Jackson‐Holmes Emily , Sun Gongchen , Zhou Ying , Siciliano Benjamin , Niu Weibo , Han Haejun , Nikitina Arina , Kemp Melissa L. , Wen Zhexing , Lu Hang 
Published: August 2024

AbstractHuman brain organoids produce anatomically relevant cellular structures and recapitulate key aspects of in vivo brain function, which holds great potential to model neurological diseases and screen therapeutics. However, the long growth time of 3D systems complicates the culturing of brain organoids and results in heterogeneity across samples hampering their applications. An integrated platform is developed to enable robust and long‐term culturing of 3D brain organoids. A mesofluidic bioreactor device is designed based on a reaction‐diffusion scaling theory, which achieves robust media exchange for sufficient nutrient delivery in long‐term culture. This device is integrated with longitudinal tracking and machine learning‐based classification tools to enable non‐invasive quality control of live organoids. This integrated platform allows for sample pre‐selection for downstream molecular analysis. Transcriptome analyses of organoids revealed that the mesofluidic bioreactor promoted organoid development while reducing cell death. This platform thus offers a generalizable tool to establish reproducible culture standards for 3D cellular systems for a variety of applications beyond brain organoids.
Advancements and Applications of Diffractive Optical Elements in Contemporary Optics: A Comprehensive Overview
Authors: Khonina Svetlana N. , Kazanskiy Nikolay L. , Skidanov Roman V. , Butt Muhammad A. 
Published: August 2024

AbstractDiffractive optical elements (DOEs) represent a revolutionary advancement in modern optics, offering unparalleled versatility and efficiency in various applications. Their significance lies in their ability to manipulate light waves with intricate patterns, enabling functionalities beyond what traditional refractive optics can achieve. DOEs find widespread use in fields such as laser beam shaping, holography, optical communications, and imaging systems. By precisely controlling the phase and amplitude of light, DOEs can generate complex optical structures, correct aberrations, and enhance the performance of optical systems. Moreover, their compact size, lightweight nature, and potential for mass production make them indispensable in designing compact and efficient optical devices for diverse industrial and scientific applications. From improving the performance of laser systems to enabling innovative display technologies, DOEs continue to drive advancements in modern optics, promising even more exciting possibilities in the future. In this review, the critical importance of DOEs is illuminated and explore their profound implications in the contemporary era.
Killing Three Birds with One Stone: Energy Transfer Inducing Efficient, Zero Thermal Quenching, and Emission‐Color Tunable Phosphors
Authors: Tang Jiali , Zhang Xinyu , Liao Shuzhen , Zhu Yunfei , Han Yue , Su Hui , Qiu Zhongxian , Lian Shixun , Zhang Jilin 
Published: August 2024

AbstractRed emission phosphors with high efficiency and excellent thermal stability are essential for phosphor‐converted light‐emitting diodes (pc‐LEDs). Na2MgPO4F: Mn2+ shows a very weak red emission peak at 615 nm due to 3d–3d forbidden transition. And it exhibits a normal thermal quenching behavior. Blue‐emitting Eu2+ with anti‐thermal quenching (ATQ) is introduced to tune the emission color, emission efficiency, and thermal stability of Mn2+ in Na2MgPO4F. The emission color of Na2MgPO4F: Eu2+, Mn2+ phosphors can be tuned by increasing the Mn2+ content. The internal and external quantum efficiencies of Na2MgPO4F:0.03Eu2+, 0.05Mn2+ are 89.3% and 41.1%, respectively, which are much higher than those of the Mn2+‐doped ones. Furthermore, the ATQ effect of Eu2+ is also transferred to Mn2+ via energy transfer, which results in Na2MgPO4F: Eu2+, Mn2+ phosphors with zero thermal quenching (ZTQ). The cooperation of energy transfer, enhanced absorption, and increased defects amount promotes the achievement of ZTQ in the co‐doped samples. Two white pc‐LEDs with a color rendering index of more than 90 are manufactured by using the as‐synthesized Na2MgPO4F: Eu2+, Mn2+ phosphors combined with near‐UV chips. This study not only provides high‐performance Eu2+, Mn2+ co‐doped phosphors suitable for high‐quality solid‐state lighting, but also exhibits a killing‐three‐birds‐with‐one‐stone strategy to obtain efficient, thermally stable, and color‐tunable phosphors.
PRKAG2‐Related Lethal Congenital Glycogen Storage Disease of the Heart as Rare Cause of Fetal Hydrops With Bradycardia and Cardiomyopathy: Clinical Report and Literature Review
Authors: White‐Brown Alexandre M. , Richard Marilyn , Morency Anne‐Maude , Maedler‐Kron Chelsea , De Bie Isabelle 
Published: August 2024

ABSTRACTNonimmune foetal hydrops is a prenatal condition associated with significant perinatal mortality. It has so far been associated with over 200 chromosomal and monogenic conditions, most frequently chromosomal aneuploidies and RASopathies. Thorough clinical phenotyping and genetic evaluation are essential to determine the underlying etiology of this clinical entity and guide obstetrical and postnatal management. In this report, we describe the prenatal presentation and postnatal outcome of a pregnancy with Lethal Congenital Glycogen Storage Disease of the Heart, a rare autosomal dominant non lysosomal cardiac glycogenosis caused by a novel de novo likely pathogenic variant in the Protein Kinase AMP‐Activated Non‐Catalytic Subunit Gamma 2 (PRKAG2) gene, [NM_016203.3:c.1150A > G, p.(Arg384Gly)]. To this day, only six other molecularly confirmed prenatal presentations of this condition have been reported. This clinical report adds to the knowledge on the prenatal features, clinical evolution, molecular diagnosis and pathological findings of this disorder and underlines the clinical utility of comprehensive molecular testing in the investigation of nonimmune foetal hydrops and fetal cardiomyopathy.
Bridging the gap: Multi‐omics profiling of brain tissue in Alzheimer's disease and older controls in multi‐ethnic populations
Authors: Reddy Joseph S. , Heath Laura , Linden Abby Vander , Allen Mariet , Lopes Katia de Paiva , Seifar Fatemeh , Wang Erming , Ma Yiyi , Poehlman William L. , Quicksall Zachary S. , Runnels Alexi , Wang Yanling , Duong Duc M. , Yin Luming , Xu Kaiming , Modeste Erica S. , Shantaraman Anantharaman , Dammer Eric B. , Ping Lingyan , Oatman Stephanie R. , Scanlan Jo , Ho Charlotte , Carrasquillo Minerva M. , Atik Merve , Yepez Geovanna , Mitchell Adriana O. , Nguyen Thuy T. , Chen Xianfeng , Marquez David X. , Reddy Hasini , Xiao Harrison , Seshadri Sudha , Mayeux Richard , Prokop Stefan , Lee Edward B. , Serrano Geidy E. , Beach Thomas G. , Teich Andrew F. , Haroutunian Varham , Fox Edward J. , Gearing Marla , Wingo Aliza , Wingo Thomas , Lah James J. , Levey Allan I. , Dickson Dennis W. , Barnes Lisa L. , De Jager Philip , Zhang Bin , Bennett David , Seyfried Nicholas T. , Greenwood Anna K. , Ertekin‐Taner Nilüfer 
Published: August 2024

AbstractINTRODUCTIONMulti‐omics studies in Alzheimer's disease (AD) revealed many potential disease pathways and therapeutic targets. Despite their promise of precision medicine, these studies lacked Black Americans (BA) and Latin Americans (LA), who are disproportionately affected by AD.METHODSTo bridge this gap, Accelerating Medicines Partnership in Alzheimer's Disease (AMP‐AD) expanded brain multi‐omics profiling to multi‐ethnic donors.RESULTSWe generated multi‐omics data and curated and harmonized phenotypic data from BA (n = 306), LA (n = 326), or BA and LA (n = 4) brain donors plus non‐Hispanic White (n = 252) and other (n = 20) ethnic groups, to establish a foundational dataset enriched for BA and LA participants. This study describes the data available to the research community, including transcriptome from three brain regions, whole genome sequence, and proteome measures.DISCUSSIONThe inclusion of traditionally underrepresented groups in multi‐omics studies is essential to discovering the full spectrum of precision medicine targets that will be pertinent to all populations affected with AD.Highlights
Accelerating Medicines Partnership in Alzheimer's Disease Diversity Initiative led brain tissue profiling in multi‐ethnic populations.
Brain multi‐omics data is generated from Black American, Latin American, and non‐Hispanic White donors.
RNA, whole genome sequencing and tandem mass tag proteomicsis completed and shared.
Multiple brain regions including caudate, temporal and dorsolateral prefrontal cortex were profiled.
Mirror‐Image Random Nonstandard Peptides Integrated Discovery (MI‐RaPID) Technology Yields Highly Stable and Selective Macrocyclic Peptide Inhibitors for Matrix Metallopeptidase 7
Authors: Ghareeb Hiba , Yi Li Choi , Shenoy Anjana , Rotenberg Naama , Shifman Julia M. , Katoh Takayuki , Sagi Irit , Suga Hiroaki , Metanis Norman 
Published: August 2024

AbstractMatrix metallopeptidase 7 (MMP7) plays a crucial role in cancer metastasis and progression, making it an attractive target for therapeutic development. However, the development of selective MMP7 inhibitors is challenging due to the conservation of active sites across various matrix metalloproteinases (MMPs). Here, we have developed mirror‐image random nonstandard peptides integrated discovery (MI‐RaPID) technology to discover innate protease‐resistant macrocyclic peptides that specifically bind to and inhibit human MMP7. One identified macrocyclic peptide against D‐MMP7, termed D20, was synthesized in its mirror‐image form, D’20, consisting of 12 D‐amino acids, one cyclic β‐amino acid, and a thioether bond. Notably, it potently inhibited MMP7 with an IC50 value of 90 nM, and showed excellent selectivity over other MMPs with similar substrate specificity. Moreover, D’20 inhibited the migration of pancreatic cell line CFPAC‐1, but had no effect on the cell proliferation and viability. D’20 exhibited excellent stability in human serum, as well as in simulated gastric and intestinal fluids. This study highlights that MI‐RaPID technology can serve as a powerful tool to develop in vivo stable macrocyclic peptides for therapeutic applications.
Sternal haematoma infusion catheter: a novel technique for pain management in manubriosternal fractures in the emergency department
Authors: Frowde K. , Naeem S. , Alzarrad A. , Abdel‐Aziz D. , Schofield O.W. 
Published: August 2024

SummarySternal fractures are associated with significant morbidity and mortality, with some patients requiring admission for pain management, often through systemic analgesia, which may be ineffective. Regional anaesthetic techniques are more challenging for sternal fractures than rib fractures and require experienced clinicians. Local anaesthetic techniques are becoming recognised as a modality to improve pain control and to reduce complications from opioid use, especially in the elderly. We delivered local anaesthetic via a sternal haematoma infusion catheter for an elderly patient with uncontrolled pain despite the provision of intravenous patient‐controlled analgesia. This technique enabled an improvement in pain scores, better engagement with physiotherapy and reduced opioid use. Local anaesthesia has been used previously to manage pain after coronary artery bypass graft surgery. Our experience demonstrated the safety, efficacy and tolerability of this approach to analgesia in sternal fractures.
Unveiling the Synergistic Role of Ferroelectric Polarization and Z‐Scheme Heterojunction in Bi2Fe4O9/Carbon‐Deficient g‐C3N4 for Enhanced Methylene Blue Degradation Efficiency
Authors: Liang Xing , Jiang Guojian , Huang Chugeng , Xiong Zizhou , Wu Xiongjun 
Published: August 2024

ABSTRACTEnhancing the efficiency of charge carrier separation is crucial for improving the performance of photocatalysts, thereby offering more effective solutions to energy and environmental pollution challenges. In this study, a carbon‐deficient ultra‐thin porous g‐C3N4 (Vc‐UPCN) was synthesized and subsequently was integrated with Bi2Fe4O9 (BFO) using a sonochemical self‐assembly technique, a Bi2Fe4O9/Vc‐UPCN (BC) photocatalyst featuring a Z‐scheme heterojunction. The BC catalyst was subjected to corona poling treatment to obtain BCp, which possesses an intrinsic electric field. Compared with pure BFO and Vc‐UPCN, the BC heterojunction exhibited higher photo‐generated electron–hole separation efficiency and photodegradation ability. Upon introduction of corona polarization, the photocatalytic performance of BC was further enhanced. Specifically, BCp‐15 (BFO/BC mass fraction = 15) achieved complete degradation of methylene blue (MB) within 30 min. BCp‐15 demonstrated that its degradation efficiency for MB was 1.28 times higher than that of BC‐15, 1.85 times higher than that of Vc‐UPCN, and impressive 7.69 times higher than that of pure BFO. The coupling effect of the heterojunction and ferroelectric polarization significantly improved the separation efficiency of photo‐generated carriers in BCp. This study is expected to provide a reference for the synergistic application of heterojunction and ferroelectric polarization in traditional semiconductor photocatalysis.
A Review on Synthesis and Biological Activities of 2‐Aminophenol‐Based Schiff Bases and Their Transition Metal Complexes
Authors: Rana Md. Sohel , Islam Md. Azharul , Islam Md. Shohidul , Sarafi Md. Shaharul , Kudrat‐E‐Zahan Md. , Hossen Md. Faruk , Asraf Md. Ali 
Published: August 2024

ABSTRACT2‐Aminophenol‐based Schiff bases and their metal complexes have drawn a lot of interest because of their wide range of biological activity and their uses in several fields. These Schiff bases are synthesized through the condensation of 2‐aminophenol and various carbonyl compounds, and metal complexes formed easily by coordinating Schiff bases with transition metal ions possess a wide range of intriguing properties that have rendered them promising candidates in biomedical research. These compounds have a diverse array of captivating biological properties including antibacterial, antifungal, anticancer, and antioxidant actions. Their versatile nature and potential for tailored modifications hold the promise for innovative biomedical applications. Compared to standard antibiotics and other studied compounds, C2, C8–C11, and C116 complexes, along with L16 and L17 ligands, exhibit the highest antibacterial activity, while C3 and C115 complexes demonstrate the highest antifungal activity. The metal chelate formation of the ligands enhanced the antimicrobial activity by increasing their lipophilic nature. C66, C76, and C112 complexes and L39 ligand showed higher anticancer activity than standard anticancer drugs. The metal complexes have higher anticancer activity than the ligand due to improved permeability and cellular uptake. The anticancer activity of the complexes is related to the substituents of the Schiff base ligands. In compounds containing an electron‐withdrawing group (e.g., Cl or Br or NO2), the activity is obviously better than others. Ligands L1–L3 and L5, L6, and L32 demonstrated the most potent antioxidant activity, surpassing both metal complexes and standard conventional antioxidants. The ligands showed greater antioxidant activity than the metal complexes due to their capability of donating free protons to scavenge free radicals. More study is needed to fully explore the potential of 2‐aminophenol‐based Schiff bases and their transition metal complexes for the development of new drugs. This review summarizes different synthetic methods, biological activities, and the structure–activity relationship. This study aims to conduct further research on 2‐aminophenol‐based Schiff bases and their metal complexes.
Experimental study on the cushioning energy absorption characteristics of polymer materials resistant to seawater erosion in seismic damping layers
Authors: Shi Kunming , Guo Chengchao , Sun Bo 
Published: August 2024

AbstractPolymer materials exhibit vibration damping properties, yet scant research exists on their applicability to submarine tunnels. This study investigates the dynamic characteristics of Polymer Materials Resistant to Seawater Erosion (hereafter referred to as PMRSE) under varying conditions of density, confining pressure, strain rate, and erosion duration through dynamic triaxial tests. The results reveal an increase in material strength with a rise in density; enhanced strength and ductility with increasing confining pressure; and augmented strength and yield stress in correspondence with heightened strain rates. As confining pressure ascends, the equivalent damping ratio of PMRSE gradually diminishes. SEM and EDS indicate a porous structure for PMRSE, with a molded surface skin formed post‐manufacturing to thwart seawater erosion. The strain energy storage and energy absorption evaluation of PMRSE demonstrate its excellence as an energy‐absorbing material. Eventually, employing a numerical simulation model for a specific submarine tunnel reveals that the presence of a damping layer absorbs seismic energy and enhances the stress conditions of the secondary lining. PMRSE manifests as a strain‐rate sensitive material unaffected by seawater corrosion, which exhibits deformation characteristics of low yield strength and long yield stage. Accordingly, PMRSE proves suitable for vibration damping in submarine tunnels.
Donor‐Acceptor‐Donor Dyads with Electron‐Rich π‐Extended Azahelicenes to Panchromatic Absorbing Dyes
Authors: Hiroto Satoru , Chujo Moeko 
Published: August 2024

AbstractPanchromatic dyes have been highly useful in the realm of optical devices. Here, we report that panchromatic dyes with heterohelicenes have been successfully synthesized using a donor‐acceptor strategy. Our synthesis resulted in the creation of π‐extended aza[5]helicene oligomers with butadiyne linkages, which displayed bathochromically shifted absorption and emission spectra. The solvent‐dependent optical measurements revealed the intramolecular charge transfer characteristic of these molecules, and theoretical calculations described the biased molecular orbitals on the azahelicene units that generated the charge‐transfer characteristic. Encouraged by these results, we also prepared donor‐acceptor‐donor dyads using azahelicenes and dimide derivatives, resulting in panchromatic absorbing characteristics covering the range from 250 nm to 800 nm. Theoretical calculations showed the presence of mixed charge‐transfer transitions and localized transitions on the azahelicene units, which led to a broad light‐absorbing property covering the near IR region. Additionally, we conducted measurements of circular dichroism and circularly polarized luminescence for the obtained products. The g‐values were reduced by oligomerization, indicating that the lowest energy transitions were allowed in nature.
An evaluation of convergent intraverbal instruction on tacts of features, function, or class
Authors: Brown Alexandria , Cariveau Tom , Lewis Taylor K. , Ellington Paige 
Published: August 2024

AbstractEducators and parents are encouraged to arrange language‐rich environments, which provide children with exposure to language that is diverse in form and function and with repeated opportunities to emit verbal responses under a variety of conditions. Intraverbal relations constitute a large portion of these verbal interactions and may include compound antecedent verbal stimuli. Prior research has shown that responding may come under the control of limited features of compound antecedent stimuli, which may be evident when responding does not occur in the presence of individual elements or emergent performances (e.g., symmetrical relations) are absent. The current study evaluated the effects of alternating convergent intraverbal (CIV) and tact by feature, function, or class sessions on emergent tact performances in a game‐like arrangement. Participants included four children exhibiting expressive language deficits. The results revealed that correct responding on CIV trials did not consistently predict tact performances for the same targets. These findings highlight the need for additional research on the effective arrangement of compound stimuli in early educational interventions.
Exploring the Lower Limit of Target Concentration in Capture‐SELEX Using Guanine as a Model Target
Authors: Ding Yuzhe , Zhang Ziyu , Liu Juewen 
Published: August 2024

AbstractDuring an aptamer selection, using a lower target concentration may result in aptamers with a higher binding affinity. Consequently, this begs the question of whether there is a lower limit for target concentration. In this work, we conducted three aptamer selections using 5 μM, 500 nM and 50 nM guanine as the targets, respectively. Successful enrichment of the same guanine aptamers was achieved at both 5 μM and 500 nM guanine, but not with 50 nM. Using 5 μM guanine, the aptamer was enriched in eight rounds of selection, compared to that for 500 nM, which was accomplished in 17 rounds. We discuss the relation of optimal target concentration to the observed Kd value of the resulting aptamers, of which the highest affinity aptamer had a measured Kd of 200 nM. Additionally, we investigated the binding of the aptamers through mutation studies, revealing a critical cytosine. Mutating this cytosine to a thymine switched the selectivity from guanine to adenine, which is reminiscent of the guanine riboswitch. This study revealed a limit in using low target concentration, and the insights described in this article will be useful for guiding the choice of target concentration during capture‐SELEX.
Nuclear‐Targeting Peptides for Cancer Therapy
Authors: Yi Xuan , Hussain Irshad , Zhang Peng , Xiao Chunsheng 
Published: August 2024

AbstractNucleus is the central regulator of cells that controls cell proliferation, metabolism, and cell cycle, and is considered the most important organelle in cells. The precision medicine that can achieve nuclear targeting has achieved good therapeutic effects in anti‐tumor therapy. However, the presence of biological barriers such as cell membranes and nuclear membranes in cells limit the delivery of therapeutic agents to the nucleus. Therefore, developing effective nuclear‐targeting drug delivery strategies is particularly important. Nuclear‐targeting peptides are a class of functional peptides that can penetrate cell membranes and target the nucleus. They mainly recognize and bind to the nuclear transport molecules (such as Importin‐α/β) and transport the therapeutic agents to the nucleus through nuclear pore complexes (NPC). This review summarizes the most recent developments of strategies for anti‐tumor therapy utilizing nuclear‐targeting peptides, which will ultimately contribute to the development of more effective nuclear‐targeting strategies to achieve better anti‐tumor outcomes.