bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with recurrence of postoperative meningioma: a clinical study of 138 patients
Authors: Gobran Alfotih , Li Fang Cheng , Xu Xin Ke , Zhang Shang Yi 
Published: January 2014

Abstract
Background and purpose: As the postoperative recurrence of meningioma can have devastating effects, it is important to identify factors that can be used to predict this type of tumor recurrence.
Material and methods: We have performed a retrospective study involving 138 patients who were surgically treated for intracranial meningioma between 2005 and 2010. The patient population consisted of 80 females and 58 males, with an age range of 23 to 77 years old (mean age = 52 years). We measured associations between meningioma recurrence and clinical features (age, sex, extent of resection, histological type), or radiological features (tumor size, location, shape, calcification, bone changes, brain tumor interface, tumor necrosis, and MRI enhancement).
Results: We found statistically higher levels of postoperative meningioma recurrence in patients with high Simpson grade resections, high histological types, tumor diameters > 5 cm, tumors located in the base of the skull or the parasagittal sinus, tumors with unclear border, irregular enhancements on MRI, edema around the tumor, bleeding and necrosis within the tumor, and irregularly shaped tumors (e.g., lobulated or mushroom-shaped). In addition, calcification, age, sex, bone change, and dural tail sign provided no predictive value. C
onclusions: We have shown that histological type, degree of resection, bleeding, necrosis, edema, irregular enhancement, location, shape, size, and margin serve as strong predictors for the postoperative recurrence of meningioma tumors.
Fouling of nanofiltration membranes used for separation of fermented glycerol solutions
Authors: Bastrzyk Justyna , Gryta Marek , Karakulski Krzysztof 
Published: January 2014

AbstractIn this study, the glycerol solutions were fermented using Lactobacillus casei bacteria. The broths were pre-treated by microfiltration, followed by a further separation with nanofiltration. The latter process was carried out in two stages, using the NF270 and NF90 membranes, respectively. The concentrates thus obtained were enriched with citric acid (first stage) and then with lactic acid and glycerol (second stage). By means of SEM and AFM microscopy, as well as ATR-FTIR analysis, the intensity of membrane-fouling was studied. The colloidal fouling and bio-fouling caused a more than two-fold decrease in the permeate flux during microfiltration of the broth. This pre-treatment stage was effective, and a permeate turbidity of less than 0.2 NTU was obtained. However, the nanofiltration membranes exhibited a 30 % flux decline over the course of the process, mainly due to the organic fouling.
IDO-Independent Suppression of T Cell Effector Function by IFN-γ–Licensed Human Mesenchymal Stromal Cells
Authors: Chinnadurai Raghavan , Copland Ian B. , Patel Seema R. , Galipeau Jacques 
Published: January 2014

Abstract
Human bone marrow–derived mesenchymal stromal cells (MSCs) inhibit proliferation of activated T cells, and IFN-γ plays an important role in this process. This IFN-γ–licensed veto property is IDO-dependent. To further decipher the mechanistic underpinnings of MSC veto function on T cells, we investigated the effect of MSCs and IFN-γ–licensed MSCs on T cell effector function as assayed by cytokine secretion of T cells. Although MSCs and IFN-γ–licensed MSCs inhibit T cell proliferation, only IFN-γ–licensed MSCs significantly inhibit Th1 cytokine (IFN-γ, TNF-α, and IL-2) production by T cells. Additionally, IFN-γ–licensed MSCs inhibit T cell degranulation as well as single, double, and triple cytokine–producing T cells. Although IFN-γ–licensed MSCs upregulate their IDO activity, we found that MSC IDO catalytic function is dispensable with regard to MSC-driven inhibition of T cell effector function. Novel flow cytometry based functional screening of MSC-expressed, IFN-γ–licensed inhibitory molecules identified B7H1 and B7DC/PD1 pathways as essential effectors in blocking T cell function. Small interfering RNA–mediated blocking of B7H1 and B7DC reverses the inhibitory potential of IFN-γ–licensed MSCs on T cell effector function. Mechanistic analysis revealed that clustering of MHC and coinhibitory molecules are indispensable for the inhibitory effect of IFN-γ MSCs. Although exogenous IL-2 reverses B7H1-Ig–mediated inhibition of T cell proliferation, it does not affect the veto function of IFN-γ MSCs on both T cell proliferation and effector function. Our results reveal a new immunosuppressive property of IFN-γ–licensed MSCs that inhibits T cell effector function independent of IDO but through the ligands for PD1.
Oncotype Dx Results in Multiple Primary Breast Cancers
Authors: Toole Michael J. , Kidwell Kelley M. , Van Poznak Catherine 
Published: January 2014

Purpose To determine whether multiple primary breast cancers have similar genetic profiles, specifically Oncotype Dx Recurrence Scores, and whether obtaining Oncotype Dx on each primary breast cancer affects chemotherapy recommendations. Methods A database of patients with hormone receptor-positive, lymph node-negative, breast cancer was created for those tumors that were sent for Oncotype Dx testing from the University of Michigan Health System from 1/24/2005 to 2/25/2013. Retrospective chart review abstracted details of tumor location, histopathology, distance between tumors, Oncotype Dx results, and chemotherapy recommendations. Results Six hundred and sixty-six patients for whom Oncotype Dx testing was sent were identified, with 22 patients having multiple breast tumor specimens sent. Of the 22 patients who had multiple samples sent for analysis, chemotherapy recommendations were changed in 6 of 22 patients (27%) based on significant differences in Oncotype Dx Recurrence Scores. Qualitatively, there seems to be a greater difference in genetic profile in tumors appearing simultaneously on different breasts when compared to multiple tumors on the same breast. There was no association between distance between tumors and difference in Oncotype Dx scores for tumors on the same breast. Conclusions Oncotype Dx testing on multiple primary breast cancers altered management in regards to chemotherapy recommendations and should be considered for multiple primary breast cancers.
Seasonal cycles of water‐soluble organic nitrogen aerosols in a deciduous broadleaf forest in northern Japan
Authors: Miyazaki Yuzo , Fu PingQing , Ono Kaori , Tachibana Eri , Kawamura Kimitaka 
Published: January 2014

AbstractThe seasonal variations in aerosol water‐soluble organic nitrogen (WSON) concentrations measured in a deciduous forest canopy over an approximately 30 month period were investigated for possible sources in the forest. The WSON concentrations (average 157 ± 127 ng N m−3) and WSON/water‐soluble total nitrogen mass fractions (average 20 ± 11%) in the total suspended particulate matter exhibited a clear seasonal cycle with maxima in early summer. The WSON mass was found to reside mostly in the fine‐mode size range (Dp < 1.9 µm) during the summer months. WSON was positively correlated with oxidation products of α‐pinene and isoprene with similar size distributions, suggesting that secondary formation from biogenic hydrocarbon precursors is a plausible source for WSON in summer. In contrast, the majority of WSON in autumn was associated with coarse fraction (Dp > 1.9 µm), which was similar to the size distributions of sugar compounds, indicating that the major WSON sources in autumn are associated with primary biological emissions. The vertical differences in WSON concentrations suggest that the water‐soluble organic aerosol is enriched with nitrogen below the canopy level relative to the forest floor. The WSON concentration increased with enhanced hydrogen ion concentrations in aerosol in the early summer, indicating that aerosol acidity associated with anthropogenic sources outside the forest likely plays an important role in the formation of WSON in that season. The study suggests that multiple sources of WSON within the forest canopy may dominate over others in specific seasons, providing insights into WSON formation processes in forest environments.
Simulation of Internal Recycling of Adsorption Heat with Phase Change Materials
Authors: Horstmeier Jan F. , Heib Stefanie , Agar David W. 
Published: January 2014

AbstractLatentwärmespeicher ermöglichen die latente Speicherung großer Wärmemengen durch einen Phasenwechsel bei konstantem Temperaturniveau. Die beim Einsatz von Amin‐funktionalisierten Adsorbentien zur CO2‐Adsorption hohe freiwerdende Adsorptionswärme, die zu einer Minderung der Gleichgewichtskapazität führt, kann so gespeichert und bei der Desorption in einer Vakuumwechseladsorption wieder zur Regeneration des Adsorbens zur Verfügung gestellt werden. Die Integration verringert Temperaturschwankungen und macht den Prozess effizienter, wobei durch unterschiedliche Lokalisierung der Phasenwechselmaterialien im Adsorber die Effizienzsteigerung beeinflusst werden kann.
Elucidating the genetic basis for Escherichia coli defense against silver toxicity using mutant arrays
Authors: Xiu Zongming , Liu Yuanyuan , Mathieu Jacques , Wang Jing , Zhu Dongqiang , Alvarez Pedro J.J. 
Published: January 2014

Abstract

Bacterial adaptation and defense mechanisms against silver are poorly understood at the genetic level. A library of Escherichia coli gene-deletion mutants was used to show that clones lacking sodB (coding for oxidative stress protection), lon (protein damage repair), or cusR (metal efflux pump) are quite sensitive to silver (with 7.3 ± 9.1%, 5.3 ± 1.8%, and 0.4 ± 0.1% of cells surviving, respectively, compared with 90.1 ± 5.4% survival for wild-type E. coli, after 6-h exposure to 8 mg/L AgNO3), suggesting the importance of the coded functions as defense mechanisms. Mutants lacking pgaB or wcaD, which code for production of extracellular polymeric substances (EPS), also showed significant (p &lt; 0.05) sensitivity to silver exposure (23.4 ± 16.2% and 23.1 ± 32.6% survival, respectively). Transmission electron microscopy (TEM) with scanning TEM/energy-dispersive X-ray spectroscopy analysis showed accumulation of silver nanoparticles within EPS, suggesting that EPS serve as a protective barrier that also immobilizes dissolved silver as silver nanoparticles. Environ Toxicol Chem 2014;33:993–997. © 2014 SETAC
Leveraging Family History in Population‐Based Case‐Control Association Studies
Authors: Ghosh Arpita , Hartge Patricia , Kraft Peter , Joshi Amit D. , Ziegler Regina G. , Barrdahl Myrto , Chanock Stephen J. , Wacholder Sholom , Chatterjee Nilanjan 
Published: January 2014

ABSTRACTPopulation‐based epidemiologic studies often gather information from study participants on disease history among their family members. Although investigators widely recognize that family history will be associated with genotypes of the participants at disease susceptibility loci, they commonly ignore such information in primary genetic association analyses. In this report, we propose a simple approach to association testing by incorporating family history information as a “phenotype.” We account for the expected attenuation in strength of association of the genotype of study participants with family history under Mendelian transmission. The proposed analysis can be performed using standard statistical software adopting either a meta‐ or pooled‐analysis framework. Re‐analysis of a total of 115 known susceptibility single‐nucleotide polymorphisms, discovered through genome‐wide association studies for several disease traits, indicates that incorporation of family history information can increase efficiency by as much as 40%. Efficiency gain depends on the type of design used for conducting the primary study, extent of family history, and accuracy and completeness of reporting.
Population pharmacokinetics of dabrafenib, a BRAF inhibitor: Effect of dose, time, covariates, and relationship with its metabolites
Authors: Ouellet Daniele , Gibiansky Ekaterina , Leonowens Cathrine , O'Hagan Anne , Haney Patricia , Switzky Julie , Goodman Vicki L. 
Published: January 2014

AbstractDabrafenib is a BRAF kinase inhibitor indicated for the treatment of BRAF V600E mutation‐positive melanoma. The population pharmacokinetics of dabrafenib, including changes over time and relevant covariates, were characterized based on results from four clinical studies using a nonlinear mixed effects model with a full covariate approach. Steady‐state exposures of dabrafenib metabolites (hydroxy‐, carboxy‐, and desmethyl‐dabrafenib) were characterized separately. The pharmacokinetics of dabrafenib were adequately described by non‐inducible and inducible apparent clearance that increased with dose and time. Total steady‐state clearance (CL/F) at 150 mg BID dose was 34.3 L/h. Based on the induction half‐life (67 hours), steady state should be achieved within 14 days of dosing. Capsule shell was the most significant covariate (55%) while sex and weight had only a small impact on exposure (<20%). The AUC ratio (hypromellose:gelatin capsule) is predicted to be 1.80 and 1.42 following single and repeat dosing, respectively. Age, renal (mild and moderate), and hepatic (mild) impairment were not significant covariates. Steady‐state pre‐dose concentration (%CV) of dabrafenib and of hydroxy‐, carboxy‐, and desmethyl‐dabrafenib at 150 mg BID were 46.6 ng/mL (83.5%), 69.3 ng/mL (64.1%), 3608 ng/mL (14.7%), and 291 ng/mL (17.2%), respectively. Capsule shell, concomitant medications, older age, and weight were predictors of metabolite exposure.
Anthocyanin profiles and biological properties of caneberry (Rubus spp.) press residues
Authors: Tumbas Šaponjac Vesna , Gironés‐Vilaplana Amadeo , Djilas Sonja , Mena Pedro , Ćetković Gordana , Moreno Diego A , Čanadanović‐Brunet Jasna , Vulić Jelena , Stajčić Slađana , Krunić Milica 
Published: January 2014

AbstractBACKGROUNDThe global interest in natural food colours shows increasing attention towards new product development to replace synthetic colourants, because of the strengthening of legislative rules and consumer awareness of synthetic additives and chemicals in food. This study was designed to evaluate anthocyanin content and biological activities of press residues from four caneberries: two raspberry (Rubus idaeus, cv. ‘Meeker’ (RM) and ‘Willamette’ (RW)) and two blackberry (Rubus fruticosus, cv. ‘Thornfree’ (BT) and ‘Čačanska bestrna’ (BC)) cultivars.RESULTSAnalysis by high‐performance liquid chromatography–diode array detection–electrospray ionization–tandem mass spectrometry identified cyanidin glycosides in all press residues, cyanidin 3‐glucoside being prevalent in BC (1360.6 mg kg−1) and BT (1397.7 mg kg−1), and cyanidin 3‐sophoroside in RM (349.2 mg kg−1) and RW (581.0 mg kg−1). Antioxidant capacity (AC), evaluated by ABTS (2,2′‐azino‐bis(3‐ethyl benzothiazoline‐6‐sulfonic acid) assay, reducing power (RP) and α‐glucosidase inhibitory potential (α‐GIP) was higher in blackberry press residues. Total anthocyanin content was in good correlation with AC (r = 0.953; P < 0.05), RP (r = 0.993, P < 0.01) and α‐GIP (r = 0.852, P < 0.15).CONCLUSIONThis study has revealed the potential for valorization of juice production byproducts for further industrial use as a rich source of bioactive compounds and natural colourants (mainly anthocyanins). Also, they can provide health‐promoting effects beyond their general organoleptic acceptance in food product development. © 2014 Society of Chemical Industry
The oral bioavailability of curcumin from micronized powder and liquid micelles is significantly increased in healthy humans and differs between sexes
Authors: Schiborr Christina , Kocher Alexa , Behnam Dariush , Jandasek Josef , Toelstede Simone , Frank Jan 
Published: January 2014

ScopeCurcumin revealed various health‐beneficial properties in numerous studies. However its bioavailability is low due to its limited intestinal uptake and rapid metabolism. The aim of our project was to develop novel curcumin formulations with improved oral bioavailability and to study their safety as well as potential sex‐differences.Methods and resultsIn this crossover study, healthy subjects (13 women, 10 men) took, in random order, a single oral dose of 500 mg curcuminoids as native powder, micronized powder, or liquid micelles. Blood and urine samples were collected for 24 h and total curcuminoids and safety parameters were quantified. Based on the area under the plasma concentration–time curve (AUC), the micronized curcumin was 14‐, 5‐, and 9‐fold and micellar curcumin 277‐, 114‐, and 185‐fold better bioavailable than native curcumin in women, men, and all subjects, respectively. Thus, women absorbed curcumin more efficiently than men. All safety parameters remained within the reference ranges following the consumption of all formulations.ConclusionBoth, the micronized powder and in particular the liquid micellar formulation of curcumin significantly improved its oral bioavailability without altering safety parameters and may thus be ideally suited to deliver curcumin in human intervention trials. The observed sex differences in curcumin absorption warrant further investigation.
Validating computer simulations of enantioselective catalysis; reproducing the large steric and entropic contributions in Candida Antarctica lipase B
Authors: Schopf Patrick , Warshel Arieh 
Published: January 2014

ABSTRACTThe prospect for computer‐aided refinement of stereoselective enzymes is further validated by simulating the ester hydrolysis by the wild‐type and mutants of CalB, focusing on the challenge of dealing with strong steric effects and entropic contributions. This was done using the empirical valence bond (EVB) method in a quantitative screening of the enantioselectivity, considering bothkcatandkcat/KMof the R and S stereoisomers. Although the simulations require very extensive sampling for convergence they give encouraging results and major validation, indicating that our approach offers a powerful tool for computer‐aided design of enantioselective enzymes. This is particularly true in cases with large changes in steric effects where alternative approaches may have difficulties in capturing the interplay between steric clashes with the reacting substrate and protein flexibility. Proteins 2014; 82:1387–1399. © 2014 Wiley Periodicals, Inc.
The basic helix–loop–helix region of the transcriptional repressor hairy and enhancer of split 1 is preorganized to bind DNA
Authors: Popovic Matija , Wienk Hans , Coglievina Maristella , Boelens Rolf , Pongor Sándor , Pintar Alessandro 
Published: January 2014

ABSTRACTHairy and enhancer of split 1, one of the main downstream effectors in Notch signaling, is a transcriptional repressor of the basic helix–loop–helix (bHLH) family. Using nuclear magnetic resonance methods, we have determined the structure and dynamics of a recombinant protein, H1H, which includes an N‐terminal segment, b1, containing functionally important phosphorylation sites, the basic region b2, required for binding to DNA, and the HLH domain. We show that a proline residue in the sequence divides the protein in two parts, a flexible and disordered N‐terminal region including b1 and a structured, mainly helical region comprising b2 and the HLH domain. Binding of H1H to a double strand DNA oligonucleotide was monitored through the chemical shift perturbation of backbone amide resonances, and showed that the interaction surface involves not only the b2 segment but also several residues in the b1 and HLH regions. Proteins 2014; 82:537–545. © 2014 Wiley Periodicals, Inc.
Pore Structure of USY Zeolites in Dependence on Steaming Condition
Authors: Lutz Wolfgang , Shutilov Roman A. , Gavrilov Vladimir Yu. 
Published: January 2014

Abstract Thermo‐chemical dealumination of zeolite Y is accompanied by formation of different mesopore types. Closed mesopores in the nuclei and open pores at the crystal surface occur due to decomposition of its corresponding frameworks which are unstable in steam. Third type of lattice mesopores is formed inside the microporous bulk after removal of aluminum atoms if the remaining hydroxyl nests dehydroxylate. This process leads to a rearrangement of the zeolite framework. In contrast to the observed phenomenon, the defects are healed by migrating of ortho‐silicic acid (H4SiO4) from the surface to the hydroxyl nests under conditions of suitable steaming. Since mesopores in USY zeolites (ultra stable Y) reduce their catalytic activity and the sorption capacity, they could be minimized by a proper treatment.
miR-429 inhibits cells growth and invasion and regulates EMT-related marker genes by targeting Onecut2 in colorectal carcinoma
Authors: Sun Yingnan , Shen Shourong , Liu Xiaoping , Tang Hailin , Wang Zeyou , Yu Zhibin , Li Xiayu , Wu Minghua 
Published: January 2014

AbstractThe 5-year survival rate for colorectal cancer is approximately 55 % because of its invasion and metastasis. The epithelial–mesenchymal transition (EMT) is one of the well-defined processes during the invasion and distant metastasis of primary epithelial tumors. miR-429, a member of the miR-200 family of microRNAs, was previously shown to inhibit the expression of transcriptional repressors ZEB1/delta EF1 and SIP1/ZEB2, and regulate EMT. In this study, we showed that miR-429 was significantly downregulated in colorectal carcinoma (CRC) tissues and cell lines. We found that miR-429 inhibited the proliferation and growth of CRC cells in vitro and in vivo, suggesting that miR-429 could play a role in CRC tumorigenesis. We also showed that downregulation of miR-429 may contribute to carcinogenesis and the initiation of EMT of CRC by targeting Onecut2. Further researches indicated that miR-429 inhibited the cells migration and invasion and reversed TGF-β-induced EMT changes in SW620 and SW480 cells. miR-429 could reverse the change of EMT-related markers genes induced by TGF-β1, such as E-cadherin, CTNNA1, CTNNB1, TFN, CD44, MMP2, Vimentin, Slug, Snail, and ZEB2 by targeting Onecut2. Taken together, our data showed that transcript factor Onecut2 is involved in the EMT, migration and invasion of CRC cells; miR-429 inhibits the initiation of EMT and regulated expression of EMT-related markers by targeting Onecut2; and miR-429 or Onecut2 is the important therapy target for CRC.
Exposure to life‐threatening stressful situations and the risk of preterm birth and low birth weight
Authors: Wainstock Tamar , Anteby Eyal Y. , Glasser Saralee , Lerner‐Geva Liat , Shoham‐Vardi Ilana 
Published: January 2014

AbstractObjectiveTo evaluate the association between exposure to life‐threatening rocket attacks and the risks of preterm birth (PTB) and low birth weight (LBW).MethodsThe present retrospective cohort study compared the outcomes of 1851 births by women exposed to rocket attacks and 2979 births by unexposed women. The timing, frequency, and intensity of exposure were calculated for each trimester and for the entire pregnancy period. Demographic and medical data were abstracted from the patients’ records.ResultsThe rates of PTB and LBW were higher among exposed than unexposed women (PTB: 9.1% versus 6.8%, P = 0.004; LBW: 7.6% versus 5.8%, P = 0.02). The rate of infants who were small for gestational age did not differ between the groups. After controlling for potential confounders, the risks for PTB and LBW remained significantly higher in the exposed group (PTB: adjusted odds ratio 1.3 [95% confidence interval, 1.1–1.7]; LBW: adjusted odds ratio 1.3 [95% confidence interval, 1.03–1.7]). There was no linear association between the intensity of exposure and the risk of PTB or LBW.ConclusionMaternal exposure to intermittent but repeated life‐threatening rocket attacks for a prolonged period might be associated with increased risks of PTB and LBW.