bluuebunny/crossref_metadata_2025_split huggingface dataset:
New fat-derived products for treating skin-induced lesions of scleroderma in nude mice
Authors: Serratrice Nicolas , Bruzzese Laurie , Magalon Jérémy , Véran Julie , Giraudo Laurent , Aboudou Houssein , Ould-Ali Djaffar , Nguyen Pierre Sébastien , Bausset Olivier , Daumas Aurélie , Casanova Dominique , Granel Brigitte , Andrac-Meyer Lucile , Sabatier Florence , Magalon Guy 
Published: June 2015

Abstract
Introduction
Scleroderma is characterized by cutaneous manifestations that mainly affect the hands, arms and face. As of today, there is no treatment for fibrotic skin lesions of scleroderma. Previously we generated and validated a model of scleroderma-like skin sclerosis in nude mice, appropriate to inject human derived products. We showed that the subcutaneous injection of micro-fat (MF), purified and injected using small caliber cannulas, have anti-fibrotic and pro-angiogenic effects and appears more suitable for the treatment of skin lesions of scleroderma compared to the gold standard (Coleman’s technique or macro-fat). Here we compared the long-term efficacy of micro-fat “enriched” with other therapeutic products including the stromal vascular fraction (SVF) of fat and platelet-rich plasma (PRP) from blood in our murine model of scleroderma.

Methods
We used 72 nude mice in this study. We formed six experimental groups: Macro-fat, MF, SVF, PRP, MF + SVF, MF + PRP. This project has three phases: i) Induction of skin sclerosis by daily subcutaneous injections of bleomycin (BLM) for 4 weeks in nude mice; ii) Purification and injection of the different cell therapy products; iii) Histological analyses done 8 weeks post-injections.

Results
MF + SVF and MF + PRP significantly reversed dermal and epidermal sclerosis (P <0.01). Macro-fat, SVF, PRP only corrected the dermal sclerosis (P <0.05). Epidermal sclerosis was reduced in treatments containing MF (P <0.01). MF was more stable. Products containing the SVF were associated with a significant increase of the local vascularization (P <0.01).

Conclusions
All tested substances were effective in treating skin-induced lesions of scleroderma with different levels of fibrosis and vascular improvement; MF derived products are more stable and SVF demonstrated better pro-angiogenic effects. The observed efficacy of this combination of products in the animal model provides a rationale for potential clinical applications to treat human disease.
Leukocyte transmigration into tissue-engineered constructs is influenced by endothelial cells through Toll-like receptor signaling
Authors: Bartaula-Brevik Sushma , Pedersen Torbjorn O , Blois Anna L , Papadakou Panagiota , Finne-Wistrand Anna , Xue Ying , Bolstad Anne Isine , Mustafa Kamal 
Published: June 2015

Abstract
Introduction
Inflammation plays a crucial role in tissue regeneration, wound healing, and the success of tissue-engineered constructs. The aim of this study was to investigate the influence of human umbilical vein endothelial cells (ECs) on leukocyte transmigration when co-cultured with primary human bone marrow-derived multipotent stromal cells (MSCs).

Methods
MSCs with and without ECs were cultured in poly (L-lactide-co-1, 5-dioxepan-2-one) (poly (LLA-co-DXO)) scaffolds for 1 week in vitro in a bioreactor system, after which they were implanted subcutaneously in non-obese diabetic/severe combined immunodeficient mice. After 1 and 3 weeks, scaffolds were retrieved, and the mRNA expression of interleukin 1-beta (IL-1β), IL-6, IL-10, hypoxia-inducible factor 1-alpha (HIF-1α), HIF-1β, and mammalian target of rapamycin was examined by real-time reverse transcription-polymerase chain reaction. Furthermore, immunofluorescent staining was performed for IL-1β, IL-6, neutrophils, and CD11b. In addition, Western blotting was done for IL-1β and IL-6. Leukocyte transmigration genes and genes in Toll-like receptor pathways, expressed by MSCs cultured in vitro with or without ECs, were further investigated with a microarray dataset.

Results
In vitro, genes involved in leukocyte transmigration and Toll-like receptor pathways were clearly influenced by the addition of ECs. Platelet/endothelial cell adhesion molecule-1 (PECAM-1) and cadherin-5 (CDH5), both genes involved in leukocyte transmigration, were expressed significantly higher in the MSC/EC group.
In vivo, the MSC/EC group showed higher mRNA expression of hypoxia-inducible factors HIF-1α and HIF-1β. The mRNA expression of anti-inflammatory cytokine IL-10 showed no significant difference, whereas the mRNA and protein expression of pro-inflammatory cytokines IL-1β and IL-6 were lower in the MSC/EC group. The quantitative analysis of immunofluorescent staining revealed a significant difference in the number of neutrophils migrating into constructs, with the highest density found in the MSC/EC group. The number of macrophages positive for IL-6 and CD11b was significantly reduced in the MSC/EC group.

Conclusions
The recruitment of leukocytes into tissue-engineered constructs with MSCs is strongly influenced by the addition of ECs via activation of leukocyte transmigration and Toll-like receptor pathways.
Reductions in behavioral deficits and neuropathology in the R6/2 mouse model of Huntington’s disease following transplantation of bone-marrow-derived mesenchymal stem cells is dependent on passage number
Authors: Rossignol Julien , Fink Kyle D , Crane Andrew T , Davis Kendra K , Bombard Matthew C , Clerc Steven , Bavar Angela M , Lowrance Steven A , Song Cheng , Witte Steven , Lescaudron Laurent , Dunbar Gary L 
Published: June 2015

Abstract
Introduction
Huntington’s disease (HD) is an autosomal dominant disorder caused by an expanded CAG repeat (greater than 38) on the short arm of chromosome 4, resulting in loss and dysfunction of neurons in the neostriatum and cortex, leading to cognitive decline, motor dysfunction, and death, typically occurring 15 to 20 years after the onset of motor symptoms. Although an effective treatment for HD has remained elusive, current studies using transplants of bone-marrow-derived mesenchymal stem cells provides considerable promise. This study further investigates the efficacy of these transplants with a focus on comparing how passage number of these cells may affect subsequent efficacy following transplantation.

Methods
In this study, mesenchymal stem cells isolated from the bone-marrow of mice (BM MSCs), were labeled with Hoechst after low (3 to 8) or high (40 to 50) numbers of passages and then transplanted intrastriatally into 5-week-old R6/2 mice, which carries the N-terminal fragment of the human HD gene (145 to 155 repeats) and rapidly develops symptoms analogous to the human form of the disease.

Results
It was observed that the transplanted cells survived and the R6/2 mice displayed significant behavioral and morphological sparing compared to untreated R6/2 mice, with R6/2 mice receiving high passage BM MSCs displaying fewer deficits than those receiving low-passage BM MSCs. These beneficial effects are likely due to trophic support, as an increase in brain derived neurotrophic factor mRNA expression was observed in the striatum following transplantation of BM MSCs.

Conclusion
The results from this study demonstrate that BM MSCs hold significant therapeutic value for HD, and that the amount of time the cells are exposed to in vitro culture conditions can alter their efficacy.
Crystal, molecular and electronic structure of (3aS,4S,9aS,9bR)-4-ethyl-2,2-dimethylhexahydro-[1,3]dioxolo[4,5-g] indolizin-7(3aH)-one
Authors: Vrábel Viktor , Sivý Július , Šafař Peter , Žúšiová Jozefína 
Published: June 2015

Abstract
Molecules of the title compound, C13H21NO3, crystallize as single enantiomers with four stereogenic centres, their absolute configuration were confirmed by anomalous dispersion effects determined by diffraction measurements on the crystals. Conformations of the pyrrolidine and 1,3-oxolane rings are close to that of an envelope, with the flap atoms displaced by −0.205 (1) and −0.449 (1) Å, respectively, from the plane of the other remaining four atoms. The central six-membered ring of the indolizine moiety adopts a nearly perfect boat conformation, with two atoms displaced by 0.575 (1) and 0.603 (1) Å from the plane of the other remaining four atoms. Crystal structure of the title compound is stabilized by C−H···O hydrogen interactions.
Determination of adulterants in adulterant-fruit spirit blends using excitation-emission matrix fluorescence spectroscopy
Authors: Jakubíková Michaela , Sádecká Jana , Májek Pavel 
Published: June 2015

Abstract
This study introduces a reliable method to detect adulteration of spirit drinks. Excitation-emission matrix (EEM) fluorescence in combination with parallel factor analysis (PARAFAC) and partial least squares (PLS) regression was used to determine the content of water and ethanol in adulterated fruit spirit samples. EEM fluorescence spectra recorded in the emission wavelength range of 315–450 nm and in the excitation wavelength range of 240–305 nm were used for PARAFAC. The model created using PARAFAC-PLS was able to predict the water and ethanol level in adulterated apple spirit with the root mean square error of prediction (RMSEP) values of 1.9 % and 1.8 %, respectively. Regarding adulterated plum spirit, the RMSEP values of 0.7 % and 3.5 % were obtained for water and ethanol, respectively. The aim of this work was to determine whether EEM-PARAFAC can be used to distinguish between plum and apple spirit. Better results were obtained for apple spirit and the method is useful also for water-apple spirit blends.
Theoretical study of the energetics of carboxylic O–H bond cleavage in the para- and meta-substituted benzoic acid derivatives
Authors: Škorňa Peter , Vagánek Adam , Poliak Peter , Klein Erik 
Published: June 2015

Abstract
Gas phase bond dissociation enthalpies (BDE) of para- and meta-substituted benzoic acids and proton affinities (PA) of their carboxylate anions were investigated using the B3LYP/6-311++G** method for 15 substituents with various electron-donating and electron-withdrawing effects. The employed computational method provided BDE and PA values in fair agreement with experimental data. The substituent effect on BDEs and PAs was analyzed in terms of Hammett constants. Found dependences exhibit satisfactory linearity and enable quick estimation of BDEs and PAs from the Hammett constants.
Synthesis, structure, thermal decomposition and spectral properties of neutral [Fe(bpta)2(etOH)2(NCS)2]
Authors: Lukačovičová Zuzana , Uhrecký Róbert , Moncoľ Ján , Lackováa Darina , Ondrejkovičová Iveta , Koman Marian , Mičicová Zuzana , Harmatová Zuzana 
Published: June 2015

Abstract
The new iron(II) complex [Fe(bpta)2(EtOH)2(NCS)2] (bpta = 3,3’-(1,2,4-thiadiazole-3,5-diyl) dipyridine; EtOH = ethanol) has been prepared by refluxing Fe(NCS)3 or Fe(NCS)2 with thionicotinamide in an ethanol solution and characterized by elemental analysis, infrared spectroscopy, thermal decomposition and X-ray structural analysis. The characterized compound is a mixture of three regioisomers resulting from differential coordination by the 3- and 5-position 2-pyridyl groups. The bpta ligand was generated in situ by the oxidation dimerization of thionicotinamide. X-ray single-crystal diffraction revealed that the crystal structure is centrosymmetric and the complex crystallizes in the triclinic space group P-1. The iron(II) atom lies in the centre of symmetry and it is octahedrally coordinated by two nitrogen atoms from two thiocyanate anions and two oxygen atoms from ethanol molecules in the equatorial plane and two nitrogen atoms of pyridine rings from two bpta ligands in the axial positions. This complex represents the second example of metal complex with bpta.
A comparison of three commercial platforms for urinary NGAL in critically ill adults
Authors: Cruz Dinna N. , Virzì Grazia Maria , Brocca Alessandra , Ronco Claudio , Giavarina Davide 
Published: June 2015

AbstractEarly biomarkers for acute kidney injury (AKI) diagnosis are needed since an increase in serum creatinine levels is a late marker. Neutrophil gelatinase-associated lipocalin (NGAL) is one of the most promising AKI biomarkers. Prior to routine clinical use, it is necessary to evaluate and validate a high-throughput commercially available method for NGAL detection. The aim of this study was to do an independent validation and comparison of the analytical performance of three different commercially available urine NGAL (uNGAL) assays.Urine samples (n=110) were obtained from various patient groups with and without AKI. All urine samples were processed using Architect NGAL assay, Siemens AdviaOverall, there was good agreement among the three assays: Spearman’s rank correlation coefficient between Architect and Vista was 0.989 (95% confidence interval [CI], 0.983–0.993), between Architect and Advia, 0.962 (95% CI, 0.937–0.977), between Vista and Advia 2400, 0.975 (95% CI, 0.961–0.984). We observed a negative bias of Architect compared with the other assays: comparing Architect to Vista, the mean bias was –55.7 ng/mL (95% CI, –74.3 to –37.0 ng/mL); comparing Architect to Advia 2400, the mean bias was –40.9 ng/mL (95% CI, –56.4 to –25.4 ng/nL). The bias is proportional to the concentration of uNGAL and is more pronounced at higher levels, while irrelevant near the tested cutoff levels of 100 and 190 ng/mL. Comparing Vista and Advia 2400, the mean bias was 10.1 ng/mL (95% CI, 1.5–18.8 ng/mL). Intra-assay imprecision was generally acceptable across all assays; coefficient of variation ranged from 0.8% to 5.3%.All three methods for uNGAL showed acceptable performance for the tested parameters and are comparable with each other at clinically relevant cutoffs. However, Architect yields lower results than the other two methods, with a bias more pronounced at higher uNGAL concentrations, suggesting additional standardization efforts will likely be necessary to better harmonize the uNGAL methods at various clinically relevant cutoffs.
Inter-Institutional Social Partnerships Between The State And The Church In Romania (With Reference To The Child Protection)
Authors: Petrică Ion 
Published: June 2015

Abstract
Compared to the European countries, the sociologic research related to religiousness and religious affiliation ranks Romania among the most religious countries, this aspect being proved also by the active positioning of the Church in society, especially in the public space. The verification of the phenomenon may be done also through our research theme, which has a content focused on social work, whose result may be used accordingly. There are publications in the field of social work also containing chapters about the Church as an institution, describing the specific activities with social character (either of philanthropy, or of empirical assistance, or even professionalised social work). Nevertheless, most papers mention the Church only in the description of some historical aspects of social work in Romania. Our topic is new because a research similar to ours has not been conducted in Romania yet, in our opinion, as in all bibliographic sources used in the writing of our paper he have found no research approaching such topics. The entire scientific endeavour starts from the formal systematic and non-systematic collaboration already existing between Churches and DGASPCs, but in order to scientifically validate this hypothesis we chose to conduct also a quantitative analysis of the data collected through a questionnaire with closed questions. The main purpose of our paper is the highlighting of the specificity of the interaction between the Church and the social work practice in Romania, through the existing partnership links between the State and the Church.
Accuracy analysis of automatic distortion correction
Authors: Kolecki Jakub , Rzonca Antoni 
Published: June 2015

Abstract
The paper addresses the problem of the automatic distortion removal from images acquired with non-metric SLR camera equipped with prime lenses. From the photogrammetric point of view the following question arises: is the accuracy of distortion control data provided by the manufacturer for a certain lens model (not item) sufficient in order to achieve demanded accuracy? In order to obtain the reliable answer to the aforementioned problem the two kinds of tests were carried out for three lens models.
Firstly the multi-variant camera calibration was conducted using the software providing full accuracy analysis. Secondly the accuracy analysis using check points took place. The check points were measured in the images resampled based on estimated distortion model or in distortion-free images simply acquired in the automatic distortion removal mode.
The extensive conclusions regarding application of each calibration approach in practice are given. Finally the rules of applying automatic distortion removal in photogrammetric measurements are suggested.
Entering the digital era of the Open Skies Treaty
Authors: Orych Agata 
Published: June 2015

Abstract
The Open Skies Treaty has been a peace-building instrument between North American and European nations for over two decades. This agreement is based on the possibility for each country-signatory of the Treaty to independently conduct observation flights and obtain aerial imagery data of the territories of other Treaty States-Parties. This imagery data was originally acquired only using traditional photographic film cameras. Together with the rapid development and advancement of digital sensor technologies, the logical step forward was to amend the Treaty provisions to allow for the use of these types of sensors during observation missions. This paper describes this transition process and highlights a number of technical problems which needed to be addressed by experts working within the Open Skies Consultative Commission workgroups.
Assessment of surface water pollutant models of estuaries and coastal zone of Quang Ninh – Hai Phong using Spot-5 images
Authors: Ke Luong Chinh , Van Trang Ho Thi , Liem Vu Huu , Tuong Tran Ngoc , Duyen Pham Thi 
Published: June 2015

Abstract
The coastal zone and estuaries of Quang Ninh and Hai Phong have great potential not only for economic development but also for protection and conservation of biodiversity and ecosystem. Nowadays, due to industrial, agricultural and anthropogenic activities signs of water pollution in the region have been found. The level of surface water pollution can be determined by traditional methods through observatory stations. However, a traditional approach to determine water contamination is discontinuous, and thereby makes pollution assessment of the entire estuary very difficult. Nowadays, remote sensing technology has been developed and widely applied in many fields, for instance, in monitoring water environments. Remote sensing data combined with information from in-situ observations allow for extraction of polluted components in water and accurate measurements of pollution level in the large regions ensuring objectivity. According to results obtained from Spot-5 imagery of Quang Ninh and Hai Phong, the extracted pollution components, like BOD, COD and TSS can be determined with the root mean square error, the absolute mean error and the absolute mean percentage error (%): ±4.37 (mg/l) 3.86 (mg/l), 27%; ±55.32 (mg/l), 48.30 (mg/l), 14%; and ±32.90 (mg/l), 23.38 (mg/l), 28%; respectively. Obtained outcomes guarantee objectivity in assessing water contaminant levels in the investigated regions and show the advantages of remote sensing applications in Resource and Environmental Monitoring in relation to Water – Air – Land.
Methodology of Lithuanian climate atlas mapping
Authors: Valiukas Donatas , Galvonaitė Audronė , Česnulevičius Algimantas 
Published: June 2015

Abstract
Climate atlases summarize large sets of quantitative and qualitative data and are results of complex analytical cartographic work. These special geographical publications summarize long term meteorological observations, provide maps and figures which characterise different climate elements. Visual information is supplemented with explanatory texts. A lot of information on short and long term changes of climate elements were provided in published Lithuanian atlases (Atlas of Lithuanian SDR, 1981; Climate Atlas of Lithuania, 2013), as well as in prepared but unpublished Lithuanian Atlas (1989) and in upcoming new national atlas publications (National Atlas of Lithuania. 1st part, 2014). Climate atlases has to be constantly updated to be relevant and to describe current climate conditions. Comprehensive indicators of Lithuanian climate are provided in different cartographic publications. Different time periods, various data sets and diverse cartographic data analysis tools and visualisation methods were used in these different publications.
Taxonomic notes on Drepanolejeunea pleiodictya (Marchantiophyta, Lejeuneaceae), a little-known Asiatic species
Authors: Inuthai Janejaree , Chantanaorrapint Sahut , Zhu Rui-Liang 
Published: June 2015

Abstract Drepanolejeunea pleiodictya Herzog (Lejeuneaceae), a rare and poorly known species from Java and Malaysia, was newly discovered in tropical montane forest in southern Thailand. It is distinguished by the ventral margin of the leaf lobe being usually irregularly dentate with one large tooth, the apex of the lobule having a straight or slightly curved tooth, ocelli 3−4(−5) seriate in the leaf lobe separated by 2−5 ordinary leaf cells, and gynoecia usually with a single pycnolejeuneoid innovation. This species is described and illustrated in detail, and its diagnostic characters and geographical distribution are briefly discussed.
Outlier reset CUSUM for the exploration of copy number alteration data
Authors: Lai Yinglei , Gastwirth Joseph L. 
Published: June 2015

AbstractCopy number alteration (CNA) data have been collected to study disease related chromosomal amplifications and deletions. The CUSUM procedure and related plots have been used to explore CNA data. In practice, it is possible to observe outliers. Then, modifications of the CUSUM procedure may be required. An outlier reset modification of the CUSUM (ORCUSUM) procedure is developed in this paper. The threshold value for detecting outliers or significant CUSUMs can be derived using results for sums of independent truncated normal random variables. Bartel’s non-parametric test for autocorrelation is also introduced to the analysis of copy number variation data. Our simulation results indicate that the ORCUSUM procedure can still be used even in the situation where the degree of autocorrelation level is low. Furthermore, the results show the outlier’s impact on the traditional CUSUM’s performance and illustrate the advantage of the ORCUSUM’s outlier reset feature. Additionally, we discuss how the ORCUSUM can be applied to examine CNA data with a simulated data set. To illustrate the procedure, recently collected single nucleotide polymorphism (SNP) based CNA data from The Cancer Genome Atlas (TCGA) Research Network is analyzed. The method is applied to a data set collected in an ovarian cancer study. Three cytogenetic bands (cytobands) are considered to illustrate the method. The cytobands 11q13 and 9p21 have been shown to be related to ovarian cancer. They are presented as positive examples. The cytoband 3q22, which is less likely to be disease related, is presented as a negative example. These results illustrate the usefulness of the ORCUSUM procedure as an exploratory tool for the analysis of SNP based CNA data.