bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Evaluations of 1,25‐Dihydroxyvitamin D3 Receptors in Fresh Layers of Epidermis and Dermis from Normal Rat Skin
Authors: Imanaka Shunji , Morimoto Shigeto , Onishi Toshio , Okada Natsuko , Kozuka Takehito , Kitano Yukio , Yoshikawa Kunihiko 
Published: April 2015

AbstractThe 1,25‐dihydroxyvitamin D3 [1,25‐(OH)2D3] receptors in fresh layers of rat epidermis and dermis were compared after separation of the two layers by treatment with dispase. After 18 h treatment with 500 U/ml dispase, the epidermal sheet was easily peeled from the dermis with forceps, without dissociation of keratinocytes. The cytotoxicity of dispase was very low. A specific binding protein for 1,25‐(OH)2D3, with a sedimentation coefficient of 3.2 S on a sucrose density gradient, was demonstrated in both layers of fresh rat skin. Scatchard analyses of cytosol binding to the hormone gave affinity constants of 0.09 ± 0.06 (mean ± SE) nM and 0.38 ±0.19 nM and binding capacities of 17.5 ± 0.5 and 14.5 ± 3.0 fmol/mg protein for the epidermis and dermis, respectively. This procedure allows simultaneous determinations of the receptor statuses of the epidermis and dermis from the same skin sample under various conditions.
Effect of Cepharanthin on Superoxide Anion (O2–) Production by Macrophages
Authors: Sawamura Daisuke , Sato Shizuo , Suzuki Mariko , Nomura Kazuo , Hanada Katsumi , Hashimoto Isao 
Published: April 2015

AbstractOur previous report showed the inhibitory action of cepharanthin on oxygen‐derived free radical production by polymorphonuclear leukocytes (PMN). In the present study, we examined the effects of cepharanthin on production of superoxide anion (O2–), one oxygen radical, by macrophages in vitro and in vivo.Phorbol myristate acetate‐induced O2– production by mouse peritoneal exudate cells (PEC), whose constituent cell types were identified as macrophages, lymphocytes, and PMN (75, 20, and less than 5%, respectively), was measured by ferricytochrome C reduction assay. Superoxide anion production by PEC, which depended mainly on the macrophage component, was inhibited 34% by 5 μg/ml cepharanthin and 85% by 50 μg/ml. These results indicate that cepharanthin suppresses O2– production by macrophages as well as by PMN. The fact that no inhibition of O2– by a xanthine‐xanthine oxidase system was observed indicates that cepharanthin is not a scavenger of O2–.Carrageenan‐induced paw edema is due to the activity of macrophages. Participation of O2– and other oxygen radicals is implicated in this edema, because the swelling is inhibited by administration of superoxide dismutase. The effects of cepharanthin on O2– production by macrophages was examined using this experimental system in mice. However, no significant inhibition of the edema by cepharanthin was observed.
A Case of Lichen Sclerosus et Atrophicus of the Vulva Effectively Treated with Oral Etretinate
Authors: Yokota Michiko , Mizuno Nobuyuki 
Published: April 2015

AbstractA 51‐year‐old female had been suffering from itching of the external genitalia and soreness on urination for one year. Swelling, leukokeratosis and erosion on the external genitalia had been seen for six months. Her clinical findings and symptoms improved markedly after eight months administration of oral etretinate (50–10 mg/day, total dose 6,080 mg).Histologically, reappearance of the rete ridges, rearrangement of squamous cells, disappearance of hydropic degeneration of basal cells, dilated lymph capillaries and dense lymphoid cell infiltration in the middermis were prominent findings after treatment.
Unusual Clinical Manifestations of Cutaneous Tuberculosis
Authors: Sehgal Virendra N. , Srivastava Govind , Ahuja Poonam , Koranne Ravindra V. , Sharma Vinay K. 
Published: April 2015

AbstractTwo unusual patients with multifocal guttate tuberculosis verrucosa cutis and necklace lupus vulgaris are described. An endeavour was made to explain these manifestations through percentages of T‐lymphocytes T4 and T8 in the peripheral blood. Mantoux tests indicating cell‐mediated immunity were strongly positive in both cases; leucocyte migration inhibition test (LMIT) values were, however, relatively low. The latter result also indicates good cell‐mediated immunity.
Trichophytic Granuloma Associated with Bullous Pemphigoid
Authors: Ueda‐Furukawa Mikiko , Kitano Yukio , Hashimoto Koji 
Published: April 2015

AbstractA 69‐year‐old Japanese man developed multiple nodular granulomatous cutaneous lesions caused by Trichophyton rubrum during treatment of bullous pemphigoid. One and a half months after the initiation of oral corticosteroid therapy, the lesions appeared. He had suffered from tinea pedis for several decades. Oral ketoconazole was given for 6 weeks with remarkable improvement. Trichophytic granuloma is a rare but important complication of bullous pemphigoid.
Pigmented Macules in Patients Treated with Systemic 5‐Fluorouracil
Authors: Cho Kwang Hyun , Chung Jin Ho , Lee Ai Young , Lee Yoo Shin , Kim Noe Kyung , Kim Chul Woo 
Published: April 2015

AbstractPigmented macules were observed in 72 (52.6%) out of 137 patients treated with systemic 5‐fluorouracil. These pigmented macules usually appeared on the palms and the soles. Their incidence seemed to be proportional to the number of chemotherapy cycles. Histological studies showed a slight increase in melanin pigmentation in the epidermis without any change in the melanocyte number. This suggests that the melanocytes already present produced quantities of melanin.
Electrophoretic Mobility of Polymorphonuclear Leukocytes Response to Scale Extracts from Psoriasis Vulgaris
Authors: Higuchi Mitsunari 
Published: April 2015

AbstractThe cell electrophoretic mobility of polymorphonuclear leukocyte responses to scale extracts from psoriasis vulgaris and other dermatoses (callus and pompholyx) was determined by analytical free‐flow cell electrophoresis technique. Incubation with scale extracts from psoriasis significantly reduced the electrophoretic mobility of polymorphonuclear leukocytes compared with those from callus and pompholyx; ultrafiltrated scale extracts separated 10,000–30,000 d showed the highest reduction. Pretreatment of polymorphonuclear leukocytes with etretinate in vitro blocked reduction of the cell electrophoretic mobility. It is concluded that psoriatic scale extracts contain factor(s) which reduce the charge density of polymorphonuclear leukocytes and that this reduction is inhibited by etretinate pretreatment.
Phorbol Ester Decreases Cytosolic Retinoic Acid Binding Protein (CRABP) Capacity in Normal but not Psoriatic Epidermis
Authors: Junquero Didier , Basset‐Seguin Nicole , Modat Guy , Levy Chantal , Guilhou Jean‐Jacques , Bonne Claude 
Published: April 2015

AbstractThe retinoic acid binding protein (CRABP) characterized in human epidermal cytosol exhibits a three‐fold increased binding capacity in lesional psoriatic epidermis as compared to normal or uninvolved skin. Treatment of epidermal homogenate from normal subjects by phorbol ester + ATP decreases the specific binding capacity of CRABP without affecting its dissociation constant (Kd=10 nM). The same effect was not observed in involved and uninvolved psoriatic epidermis. These results could be related to the previously reported decreased protein kinase C activity in psoriatic skin. They also suggest that posttranslational events could be responsible for pathological and pharmacological variations in CRABP binding capacity.
Bullous Pemphigoid of Childhood: Immunofluorescent Investigation
Authors: Tamaki Kunihiko , Igarashi Atsuyuki , Nakamura Koh‐ichiro , Nashiro Kiyoko , Okochi Hitoshi , Seki Yoshihito , Tsuchida Tetsuya 
Published: April 2015

AbstractA four‐month‐old Japanese girl with bullous pemphigoid of childhood (BPC) was reported. The characteristics of BPC antigen were studied by immunofluorescent technique using the serum of this patient with variously treated normal human skin as substrate. This study showed that her BPC antigen was quite similar to bullous pemphigoid antigen seen in adult patients and that this BPC antigen was differed from epidermolysis bullosa acquisita (EBA) antigen.
Lichenoid Eruption due to Mercaptopropionylglycine
Authors: Kawabe Yoko , Mizuno Nobuyuki , Yoshikawa Kunihiko , Matsumoto Yoshifuji 
Published: April 2015

AbstractTwo patients with lichenoid eruption due to mercaptopropionylglycine (MPG) are reported. Case 1 was a 63‐year‐old woman, who developed a pruritic eruption after oral ingestion of MPG 600 mg/day for a month and a half. The clinicopathological findings were a lichenoid reaction. She also showed diffuse alopecia, which has not been reported as a side effect of this drug. Case 2 was a 71‐year‐old man who developed discoid lupus erythematosus‐like lesions after oral MPG 200 mg/day for two months, but the histological findings were a lichenoid reaction. In both patients, the lesions disappeared rapidly after discontinuing the drug. The eruption was reproduced by readministrating MPG in case 1. The skin reaction in both patients seemed to be the cell‐mediated immune reaction to MPG.
Viral Activity in Skin Lesions of Herpes Zoster
Authors: Hata Seiichiro , Sugai Tetsuro , Asoh Satsuki , Asada Yasuo , Nishijima Setsuko , Soh Yoshiro , Doi Akira , Baba Koichi 
Published: April 2015

AbstractVaricella‐zoster virus (VZV) was isolated in 41 of 53 (77%) patients with herpes zoster on the first to ninth days after the rash. The isolation rate was highest on the early days, as high as 86% on days one and two of rash, and gradually decreased to the ninth day; the isolation rate depended more upon the condition of skin lesions than the days of illness. The high viral activity in the rash proves its important role as the source of VZV infections and the reliability of virus isolation for the diagnosis of herpes zoster.
Sweet's Syndrome in a Patient with Basedow's Disease
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Imai Toshiya , Ihara Chihiro , Ootani Atsuko 
Published: April 2015

AbstractA case of Sweet's syndrome in a patient with Basedow's disease was reported. The painful eruptions appeared on the face, trunk, and extremities, associated with high fever. The patient also had the laboratory findings of Basedow's disease. The mechanism of the co‐occurrence of Sweet's syndrome and Basedow's disease was discussed.
The Substrate of Epidermal Proteases
Authors: Takahashi Masae , Tezuka Tadashi 
Published: April 2015

AbstractIn newborn rat skin, disulfide bonds exist in the cell membrane of the stratum corneum and in the cytoplasm of the stratum granulosum. However, in the latter region, they can be detected after ethanol fixed epidermis remains for 2 weeks at 4°C. Thus, the idea arose that the visualization of the disulfide bonds in the stratum granulosum may be caused by proteases. Therefore, the in situ effects by both activation and inhibition of epidermal proteases were examined in tissue sections. Cryostat sections which were fixed in cold ethanol (—20°C, 98%) were incubated either in PBS, pH 7.4, containing 10mM 2‐ME as a control or in an epidermal homogenate (15,000×g supernatant fraction containing 2‐ME) at 37°C for 30 minutes and 4 hours, respectively, in order to activate the epidermal proteases and then reacted with NEM, 2‐ME, and DACM in steps. Many minute fluorescent particles were seen in the stratum granulosum under both of the above conditions. However, with incubation in PBS without 2‐ME, no fluorescent particles were seen. In addition, when the tissue was treated with NEM and zinc chloride before incubation, no fluorescent particles were seen. O‐phenanthrolin remarkably inhibited the appearance of these fluorescent particles. In contrast, leupeptin and antipain only slightly inhibited the appearance of these fluorescent particles, and pepstatin and phenylmethylsulfonyl fluoride didn't inhibit at all. In general, as NEM and zinc chloride strongly inhibit thiol proteases, these findings suggest that presence of the minute fluorescent particles in the stratum granulosum could be due to the effects of proteases, especially thiol proteases.
Hyperthermic Effects In vitro on the Peripheral Lymphocytes Isolated from Three Cases of Malignant Lymphoma/Leukemia
Authors: Nakayama Juichiro , Higuchi Rie , Kabemura Mayumi , Kiryu Hiromaro , Takenaka Akira , Hori Yoshiaki 
Published: April 2015

AbstractWe have carried out in vitro studies of hyperthermic effects on the viability, blastogenesis, and ultrastructural changes of atypical lymphocytes isolated from the peripheral blood of three patients with malignant lymphoma/leukemia. One was lymphocytic lymphoma/leukemia (T‐cell origin), and the others were adult T‐cell lymphoma/leukemia (both acute and smoldering type). In the cases of adult T‐cell lymphoma/leukemia, the peripheral lymphocytes were found to be more sensitive to hyperthermia than those of non‐ATL patients in terms of trypan‐blue exclusion ability and blastogenesis ability with concanavalin A challenge. When heated under sublethal conditions, lymphocytes of both ATL and non‐ATL patients exhibited the following characteristics: production of multilayered nuclear membranes, increase in vesicles (lysosomes), swelling of mitochondria, and cytoplasmic vacuolization.
Type IV Collagen Synthesis by Cultured Mouse Keratinocytes (Pam Cells)
Authors: Tokimitsu Ichiro , Ohyama Katsumi , Tajima Shingo , Nishikawa Takeji 
Published: April 2015

AbstractThe collagens synthesized by Pam cells were biochemically characterized. The collagen in the cell layers was identified as type IV collagen by immunoprecipitation with type IV collagen specific antibody. The apparent molecular weights were 180 Kd and 175 Kd, which converted to major (160 Kd) and several minor smaller fragments with pepsin treatment. The ratios of 3‐hydroxyproline/4‐hydroxyproline and 4‐hydroxyproline/proline in the 160 Kd polypeptide were 0.11 and 1.18, respectively. The result shows that Pam cells are able to produce type IV collagen whose biochemical characteristics are similar to those from other epithelial cells.In the kinetic study, the synthesis of type IV collagen reached its maximum level rapidly on the 1st day after seeding, suggesting that type IV collagen may play a role in cell attachment and proliferation.
Neonatal Lupus Syndrome and Microtubular Structure
Authors: Nitta Yukiko , Ohashi Masaru 
Published: April 2015

AbstractA female infant, at 6 weeks old, with the clinical manifestation of annular erythemas on the trunk and face, was positive for anti‐SS‐A and anti‐SS‐B antibodies. The annular erythema disappeared spontaneously at the age of 7 months when anti‐SS‐A and anti‐SS‐B antibodies were negative. Electron microscopic observation of the vascular endothelial cells on the annular erythema revealed microtubular structures. Later microtubular structures were found absent from the vascular endothelial cells of the area where the annular erythema had disappeared. The patient's mother is suffering from Sjögren's syndrome; she has no clinical symptoms but anti‐SS‐A and anti‐SS‐B antibodies are positive and a biopsy of small salivary glands of the lip demonstrated a marked periductal mononuclear cell infiltration. Microtubular structures were observed in her vascular endothelial cells in the small salivary gland region. These findings suggest that disappearance of microtubular structure may have some relationship with anti‐SS‐A and anti‐SS‐B antibodies.
Exacerbation of Psoriasis during Lithium Treatment
Authors: Sasaki Tetsuo , Saito Sumi , Aihara Michiko , Ohsawa Junko , Ikezawa Zenro 
Published: April 2015

AbstractExacerbation of psoriasis was observed in a 28‐year‐old patient following the addition of lithium carbonate to a treatment regimen of carbamazepine and other drugs for ***manic‐depressive psychosis. The biopsied lesion histologically showed a typical psoriasiform tissue reaction and immunohistochemically, remarkable infiltration of activated helper T cells. Immunologic reaction to lithium in a patch test and a lymphocyte proliferation test could not be demonstrated. The mechanisms of lithium action and their relations to the exacerbation of psoriasis were reviewed. In this patient, although we could not define which effect of lithium was most important, it seemed that an increased blood lithium level combined with simultaneous carbamazepine administration enhanced the triggering effect of lithium and caused the exacerbation of psoriasis.
A Case of Tick‐bite from Ixodes ovatus Neumann
Authors: Fukuoka Fukami , Kawasaki Yohji , Dekio Satoshi , Jidoi Joji , Sugihara Kumiko , Abe Kenji , Yamane Yosuke 
Published: April 2015

AbstractA 70‐year‐old male forestation worker had a tick‐bite on the lower abdominal skin. The bite was treated by surgical excision of the tick together with the surrounding skin. The tick was identified as Ixodes ovatus Neumann through investigation of its morphological characteristics. This patient is the fourth case of a tick‐bite from Ixodes ovatus Neumann in western Japan.
Glycosylated Proteins of Skin, Nail and Hair: Application as an Index for Long‐term Control of Diabetes Mellitus
Authors: Sueki Hirohiko , Nozaki Shigeyuki , Fujisawa Ryuichi , Aoki Kimiko , Kuroiwa Yukio 
Published: April 2015

AbstractThe purpose of the present study was to compare the degrees of nonenzymatically glycosylated proteins in the skin (stratum corneum), the nail, the hair, and hemoglobin obtained simultaneously from the same subject and to evaluate the most useful sample for management of diabetic complications. Fifty‐one diabetic patients and 20 control patients were examined, utilizing furosine determination. Furosine value of the skin in diabetics was 2.14 ± 1.70%, whereas that in controls was 1.65 ± 0.47%. Furosine value of the nail in diabetics was 6.67 ± 3.30%, whereas that in controls was 4.16 ± 1.62%. Furosine value of the hair in diabetics was 1.30 ± 1.11%, whereas that in controls was 1.29 ± 1.71%. Close correlations were detected between HbA1 (glycosylated hemoglobin) and furosine of the nail (r=0.58, p<0.001), HbA1 and furosine of the skin (r=0.48, p<0.001), and HbA1 and furosine of the hair (r=0.43, p<0.01); however, poor correlations were found between furosine of the hair and the skin (r=0.35, p<0.05) and furosine of the nail and the hair (r=0.33, p<0.05). Furosine of the nail was significantly correlated with the FBS (fasting blood sugar) of the same time, previous 6, and previous 12 months. Furosine value of the nail, we believe, is the most useful indicator for evaluating long term control of diabetics and may provide useful information for management of diabetic complications.
Histopathologic Features of Superficial Granulomatous Pyoderma
Authors: Winkelmann Richard K , Wilson‐Jones Edward , Gibson Lawrence E. , Quimby Steven R. 
Published: April 2015

AbstractStudy of the histopathologic pattern of 28 cases of superficial granulomatous pyoderma demonstrated an ulcerative, verrucous pyoderma of the superficial dermis. Focal abscesses in the subepidermis or dermis were juxtaposed with granuloma formation and plasma cell inflammation. Hemorrhage and granulation tissue were present. The clinical lesions are indolent, and this feature is confirmed by chronic inflammatory elements, including sinus tract formation and focal fibrosis. Superficial granulomatous pyoderma must be added to the histologic differential diagnosis of the vegetative and ulcerative granulomas: blastomycosis, tuberculosis verrucosa cutis, and bromoderma.
A Case of Mosquito Hypersensitivity Showing Abnormality of Peripheral Monocyte Function
Authors: Sugihara Kumiko , Yamada Yoshitaka , Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractWe report here an 11‐year‐old Japanese girl with an ulcer‐forming type of mosquito hypersensitivity. Since the lesions might have arisen from an Arthus type of hypersensitivity, we used a phorbol myristate acetate (PMA)‐triggered chentiluminescence method to investigate the function of her peripheral monocytes, which should play an important role in any Arthus reaction in association with polymorphonuclear leucocytes (PMNs). The chemiluminescence of the patient's monocytes was significantly reduced from that of the normal control. To our best knowledge, no such functional abnormality of the monocytes has been seen previously in patients with an ulcer‐forming type of mosquito hypersensitivity.
A Giant Calcifying Pseudocyst Seen in a Patient with Overlap Syndrome
Authors: Hashiro Makoto , Maeda Motomu , Kobayashi Yoichi , Yoshikawa Kunihiko 
Published: April 2015

AbstractA case of a female with a large cystic tumor in her infrascapular region is reported. The tumor was 8 cm × 7 cm in size. Histologically, it was a pseudocyst with calcification. The patient showed no abnormal renal functions other than proteinuria. Because she had suffered from overlap syndromes of systemic lupus erythematosus, progressive systemic sclerosis, and suspected dermatomyositis, we diagnosed this as a unique case of dystrophic calcinosis. There have been several reports of cases with connective tissue diseases showing subcutaneous calcification, but to our knowledge no such giant calcifying pseudocyst has ever been reported.
Arteriovenous Malformation with an Ulcer of the Foot
Authors: Matsukawa Ataru , Sakai Shigenobu 
Published: April 2015

AbstractAn unusual case of arteriovenous malformation (AVM) associated with ulcer arising on the sole of the left foot is reported. This ulcer was precipitated by pregnancy. After a split thickness skin graft repaired the ulcer, diffuse AVM was found on the left lower leg by arteriography. All the involved vessels were totally excised and the course was comparatively good. Clinical manifestations of AVM, incidences of ulcer in AVM, histological differentiation from Kaposi sarcoma, and treatment of AVM are discussed.
Rickettsioses of the Spotted Fever Group around the World
Authors: Walker David H. 
Published: April 2015

AbstractSpotted fever group rickettsioses comprise Rocky Mountain spotted fever (Rickettsia rickettsii), boutonneuse fever (R. conorii), North Asian tick typhus (R. sibirica), Queensland tick typhus (R. australis), rickettsialpox (R. akari), and Oriental spotted fever (R. japonica). Ticks or mites serve as the vector and reservoir hosts of the rickettsiae. These obligate intracellular bacteria invade vascular endothelial cells, which are damaged directly, causing increased vascular permeability. The rash usually appears in Rocky Mountain spotted fever on the third day of illness and later evolves to become petechial maculopapules in 50% of cases with involvement of the palms and soles in a similar proportion of patients. Eschar occurs in some SFG rickettsioses at the site of tick bite, but rarely in Rocky Mountain spotted fever. Diagnosis often proves difficult, and laboratory assays for antibodies to SFG rickettsiae are generally useful only in convalescence. Rickettsiae are demonstrable by diagnostic immunohistology in biopsies of rash or eschar. Empiric treatment with doxycycline, tetracycline, or chloramphenicol should be given early in the course on the basis of clinical suspicion of the diagnosis of a SFG rickettsiosis.
Analysis of Epidermal Growth Factor Receptor Gene in Psoriasis
Authors: Dosaka Naoko , Fujita Mayumi , Tanaka Toshihiro , Miyachi Yoshiki , Imamura Sadao 
Published: April 2015

AbstractEpidermal Growth Factor (EGF) plays an important role in cell proliferation. In psoriasis, increased histochemical expression of EGF receptor has been reported in the epidermis. In order to elucidate the mechanism of this increase, we studied the EGF receptor gene organization in psoriasis. DNAs were extracted from white blood cells of 5 patients with psoriasis and 5 normal controls and also from epidermis of 2 psoriatic patients and 1 normal control, and analyzed by Southern blot technique. There were no differences in the structural organization of EGF receptor gene in either white blood cells or epidermis between psoriatic patients and normal controls. These results suggest that the histochemical increase of EGF receptor in the epidermis of psoriatic patients is not due to a structural change in this gene.