bluuebunny/crossref_metadata_2025_split huggingface dataset:
Orthodontic miniscrew failure rate and root proximity, insertion angle, bone contact length, and bone density
Authors: Watanabe H. , Deguchi T. , Hasegawa M. , Ito M. , Kim S. , Takano‐Yamamoto T. 
Published: September 2012

Watanabe H., Deguchi T., Hasegawa M., Ito M., Kim S., Takano‐Yamamoto T.  Orthodontic miniscrew failure rate and root proximity, insertion angle, bone contact length, and bone density  Orthod Craniofac Res 2013; 16:44–55. © 2012 John Wiley & Sons A/SStructured AbstractObjectives –  To test the hypothesis that there is no significant correlation between miniscrew failure rate and root proximity, insertion angle, bone contact length, and bone density.Setting and Sample Population –  This study included 107 patients in whom 190 miniscrews had been placed from April 2008 to October 2009 in Tohoku University Hospital (Sendai, Japan).Materials and Methods –  Cone beam computed tomography scans (CBCT) and periapical radiographs were taken before and after miniscrew placement. Differences in root proximity, screw insertion angle, bone contact length, and bone density were statistically compared; comparisons were also made between the CBCT images and periapical radiographs.Results –  A significantly higher success rate was observed in the maxilla than in the mandible. The distance between the miniscrew and the root surface was significantly smaller in the failure group. There were no significant differences in the insertion angle, bone contact length, or bone density between the success group and the failure group. The concordance rate between the periapical dental radiographs and CBCT images was 46.5%.Conclusion –  While bone contact length, miniscrew angle, and bone density did not exert major effects on miniscrew failure, root proximity was the factor that most affected miniscrew failure, especially for miniscrews placed in the mandible. CBCT was superior to periapical dental X‐rays for evaluating the proximity of miniscrews to the root. Correction of the X‐ray attenuation coefficient value was necessary for measuring bone density using CBCT.
Human standing is modified by an unconscious integration of congruent sensory and motor signals
Authors: Luu Billy L. , Inglis J. Timothy , Huryn Thomas P. , Van der Loos H. F. Machiel , Croft Elizabeth A. , Blouin Jean‐Sébastien 
Published: September 2012

Key points
Electrical vestibular stimulation delivered at the mastoid processes evokes a reflex response in the appendicular muscles only when they are actively involved in keeping the unsupported head and body balanced.
We show that the vestibular‐evoked muscle response was present during a task that simulated the control of standing where sensory feedback was congruent with the motor‐generated expectation to balance the body, and absent when sensory feedback did not match.
The present results indicate that the task dependency of the vestibular‐evoked muscle response relies on congruent sensory and motor signals, and that this is organised in the absence of a conscious perception of postural control.
These findings help us understand how our brain combines sensory and motor signals to provide an internal representation of standing balance that can be used to assess whether a perturbation poses a postural threat.
Abstract  We investigate whether the muscle response evoked by an electrically induced vestibular perturbation during standing is related to congruent sensory and motor signals. A robotic platform that simulated the mechanics of a standing person was used to manipulate the relationship between the action of the calf muscles and the movement of the body. Subjects braced on top of the platform with the ankles sway referenced to its motion were required to balance its simulated body‐like load by modulating ankle plantar‐flexor torque. Here, afferent signals of body motion were congruent with the motor command to the calf muscles to balance the body. Stochastic vestibular stimulation (±4 mA, 0–25 Hz) applied during this task evoked a biphasic response in both soleus muscles that was similar to the response observed during standing for all subjects. When the body was rotated through the same motion experienced during the balancing task, a small muscle response was observed in only the right soleus and in only half of the subjects. However, the timing and shape of this response did not resemble the vestibular‐evoked response obtained during standing. When the balancing task was interspersed with periods of computer‐controlled platform rotations that emulated the balancing motion so that subjects thought that they were constantly balancing the platform, coherence between the input vestibular stimulus and soleus electromyogram activity decreased significantly (P < 0.05) during the period when plantar‐flexor activity did not affect the motion of the body. The decrease in coherence occurred at 175 ms after the transition to computer‐controlled motion, which subjects did not detect until after 2247 ms (Confidence Interval 1801, 2693), and then only half of the time. Our results indicate that the response to an electrically induced vestibular perturbation is organised in the absence of conscious perception when sensory feedback is congruent with the underlying motor behaviour.
Gluteal muscle activity and patellofemoral pain syndrome: a systematic review
Authors: Barton Christian J , Lack Simon , Malliaras Peter , Morrissey Dylan 
Published: September 2012

ObjectiveThere is growing evidence to support the association of gluteal muscle strength deficits in individuals with patellofemoral pain syndrome (PFPS) and the effectiveness of gluteal strengthening when treating PFPS. In additiona, an impressive body of work evaluating gluteal electromyography (EMG) has recently emerged, further supporting the importance of gluteal muscle function in PFPS. This systematic review synthesises these EMG findings in order to better understand the role of gluteal muscle activity in the aetiology, presentation and management of PFPS.MethodsMEDLINE, EMBASE, CINAHL, Web of Knowledge and Google Scholar databases were searched in September 2011 for prospective and case–control studies evaluating the association of gluteal EMG with PFPS. Two independent reviewers assessed each paper for inclusion and quality. Means and SDs were extracted from each included study to allow effect size calculations and comparison of results.ResultsTen case–control, but no prospective studies were identified. Moderate-to-strong evidence indicates gluteus medius (GMed) activity is delayed and of shorter duration during stair negotiation in PFPS sufferers. In addition, limited evidence indicates GMed activity is delayed and of shorter duration during running, and gluteus maximus (GMax) activity is increased during stair descent.ConclusionsDelayed and shorter duration of GMed EMG may indicate impaired ability to control frontal and transverse plane hip motion. Further research evaluating the value of gluteal muscle activity screening in identifying individuals most likely to develop PFPS, and the effectiveness of interventions targeting changes to gluteal muscle activation patterns is needed.
Cortactin is associated with tumour progression and poor prognosis in prostate cancer and SIRT2 other than HADC6 may work as facilitator in situ
Authors: Hou Huilian , Chen Wei , Zhao Le , Zuo Qinqin , Zhang Guanjun , Zhang Xuebin , Wang Hongyan , Gong Huilin , Li Xiaofeng , Wang Min , Wang Yili , Li Xu 
Published: September 2012

ObjectiveCortactin acts as a prominent substrate of histone deacetylases (HDACs) and plays important roles in tumour progression in several human cancers. However, the clinical significance of its expression in human prostate cancer (PCa) has not been determined. We aimed to identify the potential role of cortactin expression in tumour progression and prognosis in PCa and the association with HDACs.Methods256 foci with distinctive lesions in 110 prostate specimens were collected to identify the status of among cortactin, SIRT2, histone deacetylase 6 (HDAC6) by immunohistochemistry and its relationship with clinicopathological and follow-up data were analysed.ResultsThe results showed that cortactin expression was significantly higher (79.1%), and SIRT2 expression was lower (37.3%) in PCa foci, when it was compared with high-grade prostatic intraepithelial neoplasia foci and benign foci, respectively. HDAC6 expression was low and had no statistical significance in PCa. High intensity of cortactin staining was significantly and independently associated with a high prostate-specific antigen (PSA) level, high Gleason score, clinical stage progression and shortened survival time in patients with PCa. High intensity of SIRT2 staining was significantly and independently associated with a high PSA level, old age, high Gleason score and clinical stage progression. Multivariable Cox regression analysis showed cortactin expression was a significant prognostic factor for survival of patients with PCa (β, 0.736; 95% CI 1.371 to 3.181; p=0.001).ConclusionsThe results suggested that cortactin seems to be a satisfactory marker to predict tumour progression and survival in cases of PCa. And it may be SIRT2 rather than HADAC6 is responsible for tumour occurrence and the progression of PCa.
Management of myelofibrosis: a survey of current practice in the United Kingdom
Authors: Qureshi Moosa Rashid , MacLean Cathy , McMullin Mary Frances , Harrison Claire 
Published: September 2012

AimsTo investigate the experience of clinicians in the management of patients with myelofibrosis, current treatment options and their utility.MethodsWe used an internet-based surveyResultsOf 105 respondents (mostly consultants), 79% identified bothersome symptoms as the most common issue to be addressed, while for 19%, lack of efficacy of treatment fell into this category. The survey found that a wide variety of treatments are employed but with poor perceived efficacy.ConclusionsThis is the first UK based assessment of management of myelofibrosis. Perspectives may evolve with the inception of novel therapeutics in this field , but clinical guidelines for effective management currently recommend a wide variety of agents and suggest a limited likely success rate.
Epinephrine Infusion in the Evaluation of Unexplained Cardiac Arrest and Familial Sudden Death
Authors: Krahn Andrew D. , Healey Jeffrey S. , Chauhan Vijay S. , Birnie David H. , Champagne Jean , Sanatani Shubhayan , Ahmad Kamran , Ballantyne Emily , Gerull Brenda , Yee Raymond , Skanes Allan C. , Gula Lorne J. , Leong-Sit Peter , Klein George J. , Gollob Michael H. , Simpson Christopher S. , Talajic Mario , Gardner Martin 
Published: September 2012

Background—
Epinephrine infusion may unmask latent genetic conditions associated with cardiac arrest, including long-QT syndrome and catecholaminergic polymorphic ventricular tachycardia (VT).

Methods and Results—
Patients with unexplained cardiac arrest (normal left ventricular function and QT interval) and selected family members from the Cardiac Arrest Survivors with Preserved Ejection Fraction Registry (CASPER) registry underwent epinephrine challenge at doses of 0.05, 0.10, and 0.20 μg/kg per minute. A test was considered positive for long-QT syndrome if the absolute QT interval prolonged by ≥30 ms at 0.10 μg/kg per minute and borderline if QT prolongation was 1 to 29 ms. Catecholaminergic polymorphic VT was diagnosed if epinephrine provoked ≥3 beats of polymorphic or bidirectional VT and borderline if polymorphic couplets, premature ventricular contractions, or nonsustained monomorphic VT was induced. Epinephrine infusion was performed in 170 patients (age, 42±16 years; 49% men), including 98 patients with unexplained cardiac arrest. Testing was positive for long-QT syndrome in 31 patients (18%) and borderline in 24 patients (14%). Exercise testing provoked an abnormal QT response in 42% of tested patients with a positive epinephrine response. Testing for catecholaminergic polymorphic VT was positive in 7% and borderline in 5%. Targeted genetic testing of abnormal patients was positive in 17% of long-QT syndrome patients and 13% of catecholaminergic polymorphic VT patients.

Conclusions—
Epinephrine challenge provoked abnormalities in a substantial proportion of patients, most commonly a prolonged QT interval. Exercise and genetic testing replicated the diagnosis suggested by the epinephrine response in a small proportion of patients. Epinephrine infusion combined with exercise testing and targeted genetic testing is recommended in the workup of suspected familial sudden death syndromes.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT00292032.
Les perspectives temporelles : les connaître pour en tenir compte dans nos interventions en promotion de la santé
Authors: Lessard Lily 
Published: September 2012

Résumé La modification des habitudes de vie pour adopter des comportements sains repose notamment sur la capacité des individus et des populations à estimer les conséquences à long terme des gestes accomplis aujourd’hui. Cette capacité peut cependant varier d’une personne à l’autre, notamment en raison des perspectives temporelles (PT) adoptées. Les PT réfèrent à la tendance d’une personne ou d’un groupe de personnes à orienter ses décisions en fonction d’une vision tournée vers le passé, le présent ou le futur. Cet article vise à démystifier le concept de PT et à comprendre leur construit, afin d’identifier différentes façons d’en tenir compte dans les interventions en promotion de la santé. L’influence des PT sur la santé est principalement liée à leur capacité d’agir sur la motivation des personnes à adopter et à maintenir certains comportements. Une attention particulière doit être portée aux personnes et aux populations dont les PT sont orientées vers le présent. Elles présenteraient un plus grand risque d’adopter des comportements délétères et seraient susceptibles d’être moins sensibles aux messages qui visent la modification de ces comportements que les personnes qui adoptent une PT orientée vers le futur. En ce qui concerne leur construit, les PT sont le fruit de différentes dynamiques psychologiques et des facteurs individuels (âge, sexe, état de santé) et environnementaux (milieu familial, statut socioéconomique, éducation, culture). Parmi les moyens présentés pour tenir compte des PT dans les interventions en promotion de la santé figurent la mise en valeur des avantages à court terme d’un changement de comportement qui vise des bénéfices à long terme pour la santé, la modulation de l’intensité du soutien à l’empowerment des communautés en fonction des PT adoptées et la permutation d’une PT orientée vers le présent vers une PT orientée vers le futur.
Serpins in rice: protein sequence analysis, phylogeny and gene expression during development
Authors: Francis Sheila E , Ersoy Renan A , Ahn Joon-Woo , Atwell Brian J , Roberts Thomas H 
Published: September 2012

Abstract

Background
Most members of the serpin family of proteins are potent, irreversible inhibitors of specific serine or cysteine proteinases. Inhibitory serpins are distinguished from members of other families of proteinase inhibitors by their metastable structure and unique suicide-substrate mechanism. Animal serpins exert control over a remarkable diversity of physiological processes including blood coagulation, fibrinolysis, innate immunity and aspects of development. Relatively little is known about the complement of serpin genes in plant genomes and the biological functions of plant serpins.

Results
A structurally refined amino-acid sequence alignment of the 14 full-length serpins encoded in the genome of the japonica rice Oryza sativa cv. Nipponbare (a monocot) showed a diversity of reactive-centre sequences (which largely determine inhibitory specificity) and a low degree of identity with those of serpins in Arabidopsis (a eudicot). A new convenient and functionally informative nomenclature for plant serpins in which the reactive-centre sequence is incorporated into the serpin name was developed and applied to the rice serpins. A phylogenetic analysis of the rice serpins provided evidence for two main clades and a number of relatively recent gene duplications. Transcriptional analysis showed vastly different levels of basal expression among eight selected rice serpin genes in callus tissue, during seedling development, among vegetative tissues of mature plants and throughout seed development. The gene OsSRP-LRS (Os03g41419), encoding a putative orthologue of Arabidopsis AtSerpin1 (At1g47710), was expressed ubiquitously and at high levels. The second most highly expressed serpin gene was OsSRP-PLP (Os11g11500), encoding a non-inhibitory serpin with a surprisingly well-conserved reactive-centre loop (RCL) sequence among putative orthologues in other grass species.

Conclusions
The diversity of reactive-centre sequences among the putatively inhibitory serpins of rice point to a range of target proteases with different proteolytic specificities. Large differences in basal expression levels of the eight selected rice serpin genes during development further suggest a range of functions in regulation and in plant defence for the corresponding proteins.
Time-course microarrays reveal early activation of the immune transcriptome and adipokine dysregulation leads to fibrosis in visceral adipose depots during diet-induced obesity
Authors: Kwon Eun-Young , Shin Su-Kyung , Cho Yun-Young , Jung Un Ju , Kim Eunjung , Park Taesun , Park Jung Han Yoon , Yun Jong Won , McGregor Robin A , Park Yong Bok , Choi Myung-Sook 
Published: September 2012

Abstract

Background
Visceral white adipose tissue (WAT) hypertrophy, adipokine production, inflammation and fibrosis are strongly associated with obesity, but the time-course of these changes in-vivo are not fully understood. Therefore, the aim of this study was to establish the time-course of changes in adipocyte morphology, adipokines and the global transcriptional landscape in visceral WAT during the development of diet-induced obesity.

Results
C57BL/6 J mice were fed a high-fat diet (HFD) or normal diet (ND) and sacrificed at 8 time-points over 24 weeks. Excessive fat accumulation was evident in visceral WAT depots (Epidydimal, Perirenal, Retroperitoneum, Mesentery) after 2–4 weeks. Fibrillar collagen accumulation was evident in epidydimal adipocytes at 24 weeks. Plasma adipokines, leptin, resistin and adipsin, increased early and time-dependently, while adiponectin decreased late after 20 weeks. Only plasma leptin and adiponectin levels were associated with their respective mRNA levels in visceral WAT. Time-course microarrays revealed early and sustained activation of the immune transcriptome in epididymal and mesenteric depots. Up-regulated inflammatory genes included pro-inflammatory cytokines, chemokines (Tnf, Il1rn, Saa3, Emr1, Adam8, Itgam, Ccl2, 3, 4, 6, 7 and 9) and their upstream signalling pathway genes (multiple Toll-like receptors, Irf5 and Cd14). Early changes also occurred in fibrosis, extracellular matrix, collagen and cathepsin related-genes, but histological fibrosis was only visible in the later stages.

Conclusions
In diet-induced obesity, early activation of TLR-mediated inflammatory signalling cascades by CD antigen genes, leads to increased expression of pro-inflammatory cytokines and chemokines, resulting in chronic low-grade inflammation. Early changes in collagen genes may trigger the accumulation of ECM components, promoting fibrosis in the later stages of diet-induced obesity. New therapeutic approaches targeting visceral adipose tissue genes altered early by HFD feeding may help ameliorate the deleterious effects of diet-induced obesity.
Constitutive expression of transgenes encoding derivatives of the synthetic antimicrobial peptide BP100: impact on rice host plant fitness
Authors: Nadal Anna , Montero Maria , Company Nuri , Badosa Esther , Messeguer Joaquima , Montesinos Laura , Montesinos Emilio , Pla Maria 
Published: September 2012

Abstract

Background
The Biopeptide BP100 is a synthetic and strongly cationic α-helical undecapeptide with high, specific antibacterial activity against economically important plant-pathogenic bacteria, and very low toxicity. It was selected from a library of synthetic peptides, along with other peptides with activities against relevant bacterial and fungal species. Expression of the BP100 series of peptides in plants is of major interest to establish disease-resistant plants and facilitate molecular farming. Specific challenges were the small length, peptide degradation by plant proteases and toxicity to the host plant. Here we approached the expression of the BP100 peptide series in plants using BP100 as a proof-of-concept.

Results
Our design considered up to three tandemly arranged BP100 units and peptide accumulation in the endoplasmic reticulum (ER), analyzing five BP100 derivatives. The ER retention sequence did not reduce the antimicrobial activity of chemically synthesized BP100 derivatives, making this strategy possible. Transformation with sequences encoding BP100 derivatives (bp100der) was over ten-fold less efficient than that of the hygromycin phosphotransferase (hptII) transgene. The BP100 direct tandems did not show higher antimicrobial activity than BP100, and genetically modified (GM) plants constitutively expressing them were not viable. In contrast, inverted repeats of BP100, whether or not elongated with a portion of a natural antimicrobial peptide (AMP), had higher antimicrobial activity, and fertile GM rice lines constitutively expressing bp100der were produced. These GM lines had increased resistance to the pathogens Dickeya chrysanthemi and Fusarium verticillioides, and tolerance to oxidative stress, with agronomic performance comparable to untransformed lines.

Conclusions
Constitutive expression of transgenes encoding short cationic α-helical synthetic peptides can have a strong negative impact on rice fitness. However, GM plants expressing, for example, BP100 based on inverted repeats, have adequate agronomic performance and resistant phenotypes as a result of a complex equilibrium between bp100der toxicity to plant cells, antimicrobial activity and transgene-derived plant stress response. It is likely that these results can be extended to other peptides with similar characteristics.
Genetic analysis of polymorphisms in the kalirin gene for association with age-at-onset in European Huntington disease patients
Authors: Tsai Yu-Chun , Metzger Silke , Riess Olaf , Soehn Anne S , Nguyen Huu Phuc 
Published: September 2012

Abstract

Background
Huntington disease (HD) is caused by an expanded CAG repeat in the HD gene. Although the length of the CAG repeat strongly correlates with the age-at-onset (AAO), AAO in HD individuals may differ dramatically in spite of similar expanded CAG repeat lengths. Additional genetic or environmental factors are thought to influence the disease onset. Several modifier genes have been discovered so far but they do not fully explain the variability of AAO in HD. To potentially identify a novel genetic modifier, we analyzed single nucleotide polymorphisms (SNPs) in the kalirin (KALRN) gene. Kalirin is a protein crucially involved in spine plasticity and its interaction with huntingtin-associated protein-1 (HAP-1) and a potential protein dysfunction might contribute to spine pathogenesis in HD.

Methods
The selected SNPs were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and association of SNPs with AAO was investigated with the framework of linear models in an analysis of variance and covariance.

Results
Eleven SNPs in the kalirin gene were examined in an association study in European HD patients. The ten coding SNPs under investigation were monomorphic, whereas SNP rs10934657 in the promoter region showed a minor allele frequency >1%. An analysis of covariance together with the influence of the expanded HD allele was applied in 680 HD patients. SNP rs10934657 did not affect the AAO of the examined HD population.

Conclusions
The results did not reveal an association between the analyzed kalirin polymorphisms and the AAO in HD. However, it does not exclude other SNPs of the kalirin gene as susceptible genetic modifiers.