bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell‐free protein synthesis of a cytotoxic cancer therapeutic: Onconase production and a just‐add‐water cell‐free system
Authors: Salehi Amin S. M. , Smith Mark Thomas , Bennett Anthony M. , Williams Jacob B. , Pitt William G. , Bundy Bradley C. 
Published: September 2015

AbstractBiotherapeutics have many promising applications, such as anti‐cancer treatments, immune suppression, and vaccines. However, due to their biological nature, some biotherapeutics can be challenging to rapidly express and screen for activity through traditional recombinant methods. For example, difficult‐to‐express proteins may be cytotoxic or form inclusion bodies during expression, increasing the time, labor, and difficulty of purification and downstream characterization. One potential pathway to simplify the expression and screening of such therapeutics is to utilize cell‐free protein synthesis. Cell‐free systems offer a compelling alternative to in vivo production, due to their open and malleable reaction environments. In this work, we demonstrate the use of cell‐free systems for the expression and direct screening of the difficult‐to‐express cytotoxic protein onconase. Using cell‐free systems, onconase can be rapidly expressed in soluble, active form. Furthermore, the open nature of the reaction environment allows for direct and immediate downstream characterization without the need of purification. Also, we report the ability of a ”just‐add‐water“ lyophilized cell‐fee system to produce onconase. This lyophilized system remains viable after being stored above freezing for up to one year. The beneficial features of these cell‐free systems make them compelling candidates for future biotherapeutic screening and production.
Circular Business Model Innovation: Inherent Uncertainties
Authors: Linder Marcus , Williander Mats 
Published: September 2015

AbstractCircular business models based on remanufacturing and reuse promise significant cost savings as well as radical reductions in environmental impact. Variants of such business models have been suggested for decades, and there are notable success stories such as the Xerox product–service offering based on photocopiers that are remanufactured. Still, we are not seeing widespread adoption in industry. This paper examines causes for reluctance. Drawing on a hypothesis‐testing framework of business model innovation, we show that circular business models imply significant challenges to proactive uncertainty reduction for the entrepreneur. Moreover, we show that many product–service system variants that facilitate return flow control in circular business models further aggravate the potential negative effects of failed uncertainty reduction because of increased capital commitments. Through a longitudinal action research study we also provide a counterexample to many of the challenges identified in previous studies, which could be overcome in the studied case. Copyright © 2015 John Wiley & Sons, Ltd and ERP Environment
Environmental Policies for Evaluating Suppliers' Performance Based on GRI Indicators
Authors: Chiarini Andrea 
Published: September 2015

AbstractMany companies use a performance measurement system (PMS) to evaluate their suppliers' environmental performance (SEP). To do this a company can establish its own set of measures or adopt indicators borrowed from specific standards and guidelines such as the Global Reporting Initiative (GRI). However, the results of a literature review indicate that the weights of the different environmental performance indicators within an overall SEP rating are unclear. The aim of this paper is to understand what the environmental policies adopted by European manufacturing companies to evaluate their suppliers really are. In order to reach this objective, a quantitative model based on multiple linear regression has been developed. The model correlates different environmental aspects taken from the GRI set of indicators with the overall SEP rating. Interestingly, results show that performances on aspects such as transport and ISO 14001 certification do not count towards the final ranking, whereas compliance with laws and regulations is the cornerstone of the evaluation of the supplier. Other performance indicators can in some ways be linked to compliance with laws and regulations. Copyright © 2015 John Wiley & Sons, Ltd and ERP Environment
Supportive evidence for FOXP1,BARX1, and FOXF1 as genetic risk loci for the development of esophageal adenocarcinoma
Authors: Becker Jessica , May Andrea , Gerges Christian , Anders Mario , Veits Lothar , Weise Katharina , Czamara Darina , Lyros Orestis , Manner Hendrik , Terheggen Grischa , Venerito Marino , Noder Tania , Mayershofer Rupert , Hofer Jan‐Hinnerk , Karch Hans‐Werner , Ahlbrand Constantin J. , Arras Michael , Hofer Sebastian , Mangold Elisabeth , Heilmann‐Heimbach Stefanie , Heinrichs Sophie K. M. , Hess Timo , Kiesslich Ralf , Izbicki Jakob R. , Hölscher Arnulf H. , Bollschweiler Elfriede , Malfertheiner Peter , Lang Hauke , Moehler Markus , Lorenz Dietmar , Müller‐Myhsok Bertram , Ott Katja , Schmidt Thomas , Whiteman David C. , Vaughan Thomas L. , Nöthen Markus M. , Hackelsberger Andreas , Schumacher Brigitte , Pech Oliver , Vashist Yogesh , Vieth Michael , Weismüller Josef , Neuhaus Horst , Rösch Thomas , Ell Christian , Gockel Ines , Schumacher Johannes 
Published: September 2015

AbstractThe Barrett's and Esophageal Adenocarcinoma Consortium (BEACON) recently performed a genome‐wide association study (GWAS) on esophageal adenocarcinoma (EAC) and Barrett's esophagus. They identified genome‐wide significant association for variants at three genes, namely CRTC1, FOXP1, and BARX1. Furthermore, they replicated an association at the FOXF1 gene that has been previously found in a GWAS on Barrett's esophagus. We aimed at further replicating the association at these and other loci that showed suggestive association with P < 10−4 in the BEACON sample. In total, we tested 88 SNPs in an independent sample consisting of 1065 EAC cases and 1019 controls of German descent. We could replicate the association at FOXP1, BARX1, and FOXF1 with nominal significance and thereby confirm that genetic variants at these genes confer EAC risk. In addition, we found association of variants near the genes XRCC2 and GATA6 that were strongly (P < 10−5) although not genome‐wide significantly associated with the BEACON GWAS. Therefore, both variants and corresponding genes represent promising candidates for future EAC association studies on independent samples.
Interrupting the Intergenerational Transmission of Violence
Authors: Pinna Keri L. M. 
Published: September 2015

Children exposed to domestic violence are at increased risk for a wide range of emotional and behavioural disorders. Conduct disorder, in particular, may ultimately lead to the perpetration of further domestic violence in the next generation. Parental characteristics such as warmth and positive attributions may mitigate the risk for intergenerational transmission of violence. Sixty‐one children, ages eight to 17, and their parents completed self‐report measures of disruptive behaviour, parental warmth and parental attributions in order to determine the extent to which parental warmth and attributions of child behaviours may relate to behaviour problems that serve as risk factors for the intergenerational transmission of violence. Children were also interviewed about stressful life events to which they had been exposed, in addition to domestic violence, in order to better understand results in the context of children's lives. As predicted, parental warmth and positive attributions were related to fewer disruptive behaviour problems, r = −0.35, p = 0.04 and r = −0.32, p = 0 .05, respectively. Results were similar for boys and girls, but specific to adolescents versus younger children. Findings were maintained after accounting for other forms of adversity. Potential implications for interrupting the intergenerational transmission of violence, particularly during adolescence, are discussed.‘Parental characteristics such as warmth and positive attributions may mitigate the risk for intergenerational transmission of violence’Key Practitioner Messages

Children exposed to domestic violence are exposed to a wide range of other forms of adversity.
Cumulative adversity may contribute to disruptive behaviours that may ultimately develop into violent acts, manifesting a transmission of violence from one generation to the next.
Parental warmth and positive attributions of children's behaviours may be one way in which healthy parenting may prevent the intergenerational transmission of violence.
These parenting factors may be particularly critical during adolescence.
The ɛ‐Amino Group of Protein Lysine Residues Is Highly Susceptible to Nonenzymatic Acylation by Several Physiological Acyl‐CoA Thioesters
Authors: Simic Zeljko , Weiwad Matthias , Schierhorn Angelika , Steegborn Clemens , Schutkowski Mike 
Published: September 2015

AbstractMitochondrial enzymes implicated in the pathophysiology of diabetes, cancer, and metabolic syndrome are highly regulated by acetylation. However, mitochondrial acetyltransferases have not been identified. Here, we show that acetylation and also other acylations are spontaneous processes that depend on pH value, acyl‐CoA concentration and the chemical nature of the acyl residue. In the case of a peptide derived from carbamoyl phosphate synthetase 1, the rates of succinylation and glutarylation were up to 150 times than for acetylation. These results were confirmed by using the protein substrate cyclophilin A (CypA). Deacylation experiments revealed that SIRT3 exhibits deacetylase activity but is not able to remove any of the succinyl groups from CypA, whereas SIRT5 is an effective protein desuccinylase. Thus, the acylation landscape on lysine residues might largely depend on the enzymatic activity of specific sirtuins, and the availability and reactivity of acyl‐CoA compounds.
pH‐Cleavable Nucleoside Lipids: A New Paradigm for Controlling the Stability of Lipid‐Based Delivery Systems
Authors: Oumzil Khalid , Benizri Sébastien , Tonelli Giovanni , Staedel Cathy , Appavoo Ananda , Chaffanet Max , Navailles Laurence , Barthélémy Philippe 
Published: September 2015

AbstractLipid‐based delivery systems are an established technology with considerable clinical acceptance and several applications in human. Herein, we report the design, synthesis and evaluation of novel orthoester nucleoside lipids (ONLs) for the modulation of liposome stability. The ONLs contain head groups with 3′‐orthoester nucleoside derivatives featuring positive or negative charges. The insertion of the orthoester function in the NL structures allows the formation of pH‐sensitive liposomes. ONL‐based liposomes can be hydrolyzed to provide nontoxic products, including nucleoside derivatives and hexadecanol. To allow the release to be tunable at different hydrolysis rates, the charge of the polar head structure is modulated, and the head group can be released at a biologically relevant pH. Crucially, when ONLs are mixed with natural phosphocholine lipids (PC), the resultant liposome evolves toward the formation of a hexadecanol/PC lamellar system. Biological evaluation shows that stable nucleic acid lipid particles (SNALPs) formulated with ONLs and siRNAs can effectively enter into tumor cells and release their nucleic acid payload in response to an intracellular acidic environment. This results in a much higher antitumor activity than conventional SNALPs. The ability to use pH‐cleavable nucleolipids to control the stability of lipid‐based delivery systems represents a promising approach for the intracellular delivery of drug cargos.
Electrocatalytic Transformation of Carbon Dioxide into Low Carbon Compounds on Conducting Polymers Derived from Multimetallic Porphyrins
Authors: Dreyse Paulina , Honores Jessica , Quezada Diego , Isaacs Mauricio 
Published: September 2015

AbstractThe electrochemical reduction of carbon dioxide is studied herein by using conducting polymers based on metallotetraruthenated porphyrins (MTRPs). The polymers on glassy carbon electrodes were obtained by electropolymerization processes of the monomeric MTRP. The linear sweep voltammetry technique resulted in polymeric films that showed electrocatalytic activity toward carbon dioxide reduction with an onset potential of −0.70 V. The reduction products obtained were hydrogen, formic acid, formaldehyde, and methanol, with a tendency for a high production of methanol with a maximum value of turnover frequency equal to 15.07 when using a zinc(II) polymeric surface. Studies of the morphology (AFM) and electrochemical impedance spectroscopy results provide an adequate background to explain that the electrochemical reduction is governed by the roughness of the polymer, for which the possible mechanism involves a series of one‐electron reduction reactions.
Policy Discourses and Environmental Rationalities Underpinning India's Biofuel Programme
Authors: Chaliganti Raghu , Müller Ulrike 
Published: September 2015

AbstractThe use of renewable energy for transportation has received increasing support in environmental policy‐making around the globe. Following this trend, the Indian government launched a large‐scale biofuel programme in selected federal states, which involved the ecological rehabilitation of so‐called wastelands with energy crops. Although met with considerable opposition from civil society actors, the programme was further expanded and a national biofuel policy was adopted. Employing a critical discourse approach, this study argues that the government's strategy to promote biofuels can be explained based on technocratic design principles that have underpinned previous public policies in India. A consolidated view of these factors indicates that the continued domination of the pro‐biofuel discourse draws on a notion of weak ecological modernization. Copyright © 2015 John Wiley & Sons, Ltd and ERP Environment
Comparative Study of the Catalytic Amination of Benzylic C–H Bonds Promoted by Ru(TPP)(py)2 and Ru(TPP)(CO)
Authors: Manca Gabriele , Mealli Carlo , Carminati Daniela Maria , Intrieri Daniela , Gallo Emma 
Published: September 2015

AbstractA combined experimental and DFT‐based theoretical analysis elucidated the influence of the axial ligand L on the catalytic activity of Ru(porphyrin)L complexes in promoting the amination of benzylic C–H bonds by organic azides (RN3). Experimental data indicated that the catalytic activity of Ru(TPP)(CO) (1) (TPP = dianion of tetraphenylporphyrin) is comparable to that of Ru(TPP)(py)2 (2) (py = pyridine). DFT modelling disclosed that 2 can be regarded as a precatalyst that becomes active after the endergonic loss of one pyridine ligand to give the unsaturated species [Ru](py) (11) {[Ru] = Ru(porphine)}. This complex would react with RN3 to give the mono‐imido singlet complex [Ru](py)(NR)S (6S), which can be easily transformed into the triplet isomer 6T having diradical character at the imido N atom. The subsequent formation of the benzylic amine PhCH2NHR occurs through a radical homolytic activation of one C–H bond of the toluene substrate (PhCH3). Conversely, by staying on the singlet potential‐energy surface, 6S can undergo dissociation of the pyridine ligand to form [Ru](NR). This complex can activate another RN3 molecule to form the bis‐imido compound [Ru](NR)2, which is also catalytically active. At this point, the mechanism becomes independent of the nature of the original ligand L coordinated to [Ru].
Synthesis of a 4‐Vinyltetrahydrocarbazole by Palladium‐Catalyzed Asymmetric Allylic Alkylation of Indole‐Containing Allylic Carbonates
Authors: Pignataro Luca , Fiorito Daniele , Vece Vito , Ferraccioli Raffaella , Gennari Cesare 
Published: September 2015

AbstractPalladium‐catalyzed intramolecular asymmetric allylic alkylation was used for the first time to prepare 4‐vinyltetrahydrocarbazole 3. Suitable indole‐containing allylic carbonates were synthesized and cyclized in the presence of a chiral catalyst formed in situ from Pd(OAc)2 and members of the PhthalaPhos ligand library (i.e., 1,1′‐bi‐2‐naphthol‐monophosphites possessing a phthalic acid diamide group). The use of a stable and readily available palladium source such as Pd(OAc)2―reduced in situ by the phosphite to form the Pd0 catalyst―allowed better and more reproducible results to be obtained relative to the results achieved by using [Pd2(dba)3·CHCl3] (dba = dibenzylideneacetone) and [Pd(allyl)Cl]2. A ligand screening led to identification of the best ligand [(S)‐L1], which, after optimization of the reaction parameters, gave product 3 with 75 % ee. Remarkably, the reaction turned out to be stereodivergent: by using ligand (S)‐L1, the absolute configuration of product 3 was found to depend on the geometry of the substrate's double bond [(E)‐substrate → (S)‐3, (Z)‐substrate → (R)‐3].
Surfactant‐assisted electromembrane extraction combined with capillary electrophoresis as a novel technique for the determination of acidic drugs in biological fluids
Authors: Fakhari Ali Reza , Hasheminasab Kobra Sadat , Aladaghlo Zolfaghar , Koruni Mohammad Hosein 
Published: September 2015

In this paper, for the first time, surfactant‐assisted electromembrane extraction coupled with capillary electrophoresis with UV detector was introduced for the extraction of acidic drugs from biological fluids. In this technique, in the presence of the nonionic surfactant in the donor phase, tendency of analyte ions into the supported liquid membrane (SLM) was increased. Naproxen and diclofenac were selected as model acidic drugs. In order to obtain the best extraction efficiency, several factors influencing the extraction efficiency were investigated. Optimal extractions were accomplished with 1‐octanol as the SLM, 15 Volt dc potential as the driving force, pH 12 in acceptor solution, and 0.2 mmol/L Triton X‐100 with pH 7.4 in donor solution. Equilibrium extraction conditions were obtained after 15 min of operation where the whole assembly agitated at 1000 rpm. Under the optimized conditions, preconcentration factors in the range of 176–184 and recoveries in the range of 88–92% were obtained. The applied method offers acceptable linearity with correlation coefficients higher than 0.9992. Limits of detection of 1.51 ng/mL and 2.42 ng/mL were obtained for naproxen and diclofenac, respectively. Finally, the developed method was successfully applied for the determination of naproxen and diclofenac in different matrices including plasma and urine samples.
Effects of permeability heterogeneity on CO2 injectivity and storage efficiency coefficient
Authors: Tian Liang , Yang Zhibing , Fagerlund Fritjof , Niemi Auli 
Published: September 2015

AbstractWe study the dependency of CO2 storage efficiency coefficient (E) and injectivity index (Iinj) on the geostatistical parameters of the permeability field. CO2 injection simulations are conducted for multiple realizations of log‐normally distributed permeability fields parameterized by log permeability standard deviation (σ) and dimensionless horizontal correlation length (λ). Results show that the injectivity index increases with increasing λ, the magnitude of the effect depending on σ. Increasing σ leads to poorer injectivity for cases with small λ, but improves injectivity when λ is large. Further analysis indicates that the enhancing effect of σ on injectivity can be attributed to cases with channelized flow, while the decrease effect of σ is seen in more dispersive flow regime. The dependence of injectivity on both λ and σ is captured with a linear correlation between Iinj and a parameter group , where ξ is a dimensionless scaling parameter. The storage efficiency coefficient, on the other hand, decreases with both increasing σ and λ, and a simple linear fit is found between E and the parameter group λσ2, a well‐established heterogeneity parameter group describing e.g. macro‐dispersivity in solute transport studies. These relationships provide potentially useful tools for the preliminary evaluation of a site. Future studies should address the validity of the relationships in alternative injection scenarios and domain geometries. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd
Pleomorphic adenoma of the parotid gland 1985–2010: A Danish nationwide study of incidence, recurrence rate, and malignant transformation
Authors: Andreasen Simon , Therkildsen Marianne H. , Bjørndal Kristine , Homøe Preben 
Published: September 2015

AbstractBackgroundPleomorphic adenoma is the most frequent salivary gland tumor and is known for its tendency to recur and for its ability to transform to carcinoma ex pleomorphic adenoma (Ca‐ex‐PA). Along with pleomorphic adenoma demographics, we present the first nationwide study with long‐term follow‐up on these topics.MethodsThe Danish Pathology Data Bank was searched for parotid pleomorphic adenoma and Ca‐ex‐PA in the period 1985 to 2010 and all pathology descriptions were reviewed. Ca–ex–PA specimens were reviewed by a pathologist.ResultsA total of 5.497 patients were identified and 2.86% had at least one recurrence. An incidence of 4.29/100,000/year was found. The rate of malignant transformation in recurrent pleomorphic adenoma was 3.3%.ConclusionWe report an up‐to‐date assessment of the epidemiology of pleomorphic adenoma. We found an increasing incidence and low recurrence rate compared with previous studies. The risk of Ca‐ex‐PA for patients with recurrent pleomorphic adenoma was low compared to the literature. © 2015 Wiley Periodicals, Inc. Head Neck 38: E1364–E1369, 2016
FTIR and SEM analysis applied in tissue engineering for root recovering surgery
Authors: Costa Davidson Ribeiro , Nicolau Renata Amadei , Costa David Ribeiro , Raniero Leandro José , Oliveira Marco Antonio 
Published: September 2015

AbstractGingival recession is defined by the displacement of the gingival margin in the apical direction, which overcomes the cementum enamel junction. The etiology of gingival retraction is related to tissue inflammation caused by the accumulation of biofilm, by trauma from brushing action. Aesthetic periodontal surgery aims to return the root coverage to aesthetic harmony, and reduce the risk of periodontal disease and caries. To assist in the root coverage process, the porcine collagen matrix (PCM) has been widely studied. The objectives of this study are to identify the types of collagen that make up the PCM and analyze their morphology. For this, five PCM fragments, 2 mm (thickness) × 2.6 mm (width), were analyzed with the aid of scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR). The analysis by SEM showed that the PCM consists of two layers; the surface layer is compact, low porosity, and smooth surface, and a foamed underlying layer has high porosity. Through FTIR we identified that the surface and underlying layers are composed of collagen types I and III, respectively. This biomaterial is conducive to root coverage; it allows adsorption and cell proliferation following the matrix resorption and periodontal tissue neoformation. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 1326–1329, 2017.