bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phenotypic and genotypic characterization of Bordetella bronchiseptica strains isolated from pigs in Poland
Authors: Stępniewska K. , Urbaniak K. , Markowska-Daniel I. 
Published: September 2014

AbstractA total of 209 Bordetella bronchiseptica (Bbr) strains isolated from pigs were examined. Phenotypic study included: biochemical characterization (motility, catalase, oxidase, urease activity, nitrate reduction and growth on MacConkey agar) and antimicrobial susceptibility (disc diffusion method). Genotypic studies based on detection of three genes encoded virulence factors, such as: flagella (fla), dermonecrotoxin (dnt), and exogenous ferric siderophore receptor (bfrZ), using PCR.Most of the Bbr strains tested had a homogeneous biochemical profile. 97.6% of them provided suitable results in biochemical tests. All Bbr isolates tested showed high resistance to penicillin (100%), linco-spectin (100%) and ceftiofur (97.9%). Over 57% and 43% of Bbr strains were resistant to ampicillin and amoxicillin, respectively. All Bbr isolates showed high sensitivity to most chemotherapeutics used such as enrofloxacin (97.9%), tetracycline (97.9%), oxytetracycline (97.9%), amoxicillin with clavulonic acid (95.8%), florfenicol (90.4%), and gentamicine (77.6%). Over of 94% of Bbr strains were moderately susceptible to norfloxacine.Molecular analysis confirmed that almost all evaluated Bbr strains (94.7%) possessed the fla gene. A lower percentage of isolates had the dnt gene (72.7%) and the lowest percentage of strains (51.7%), had the bfrZ gene.
Effect of short-term rapid ventricular pacing followed by pacing interruption on arterial blood pressure in healthy pigs and pigs with tachycardiomyopathy
Authors: Skrzypczak P. , Zyśko D. , Pasławska U. , Noszczyk-Nowak A. , Janiszewski A. , Gajek J. , Nicpoń J. , Kiczak L. , Bania J. , Zacharski M. , Tomaszek A. , Jankowska E.A. , Ponikowski , P. , Witkiewicz W. 
Published: September 2014

Abstract
Ventricular tachycardia may lead to haemodynamic deterioration and, in the case of long term persistence, is associated with the development of tachycardiomyopathy. The effect of ventricular tachycardia on haemodynamics in individuals with tachycardiomyopathy, but being in sinus rhythm has not been studied. Rapid ventricular pacing is a model of ventricular tachycardia. The aim of this study was to determine the effect of rapid ventricular pacing on blood pressure in healthy animals and those with tachycardiomyopathy. A total of 66 animals were studied: 32 in the control group and 34 in the study group. The results of two groups of examinations were compared: the first performed in healthy animals (133 examinations) and the second performed in animals paced for at least one month (77 examinations). Blood pressure measurements were taken during chronic pacing - 20 min after onset of general anaesthesia, in baseline conditions (20 min after pacing cessation or 20 min after onset of general anaesthesia in healthy animals) and immediately after short-term rapid pacing. In baseline conditions significantly higher systolic and diastolic blood pressure was found in healthy animals than in those with tachycardiomyopathy. During an event of rapid ventricular pacing, a significant decrease in systolic and diastolic blood pressure was found in both groups of animals. In the group of chronically paced animals the blood pressure was lower just after restarting ventricular pacing than during chronic pacing. Cardiovascular adaptation to ventricular tachycardia develops with the length of its duration. Relapse of ventricular tachycardia leads to a blood pressure decrease more pronounced than during chronic ventricular pacing
Effect of vaginal administration of prostaglandin E2 and/or 17β-estradiol on luteal function and histological characteristics of the cervix in cyclic pigs
Authors: Przygrodzka E. , Andronowska A. , Janowski T. , Zięcik A.J. 
Published: September 2014

AbstractThe overall objective of this study was to examine the effect of vaginal administration of prostaglandin E2(PGE2) and/or17β-estradiol (E2) on luteal function maintenance and histological properties of the porcine cervix. For this purpose, crossbred gilts were divided into three groups (n=5 per group) supplied on days 11-16 of the estrous cycle with suppositories containing: (1) placebo (Group I, Control); (2) 0.4 mg of E2(Group II); (3) 0.4 mg of E2and 2 mg of PGE2(Group III). Blood samples were collected on days 11-19 of the estrous cycle to determine the concentration of progesterone (P4). Additionally, to examine local effects of the hormones applied, segments from the uterine and vaginal parts of the cervix and from the ovaries were collected post-mortem. Prolonged luteal function and extended synthesis of P4were observed in 2 of 5 gilts receiving PGE2 and E2 simultaneously (Group III). Then, these gilts were subdivided into Group IIIA (n=2; presence of corpora lutea on the ovaries) and Group IIIB (n=3; lack of corpora lutea). Increased levels of plasma P4were observed in Group IIIA on days 15-19 compared to Group IIIB and on days 16-19 compared to Group I and Group II (P<0.05; P<0.01; P<0.001, respectively). In the cervix of gilts in Groups II and III, enlarged blood vessels in the lamina propria of both parts of the cervix were observed. Furthermore, in Group II the epithelium of the uterine part of the cervix was thicker (P<0.001). Our study confirmed the proposed luteotrophic/antiluteolytic actions of E2and PGE2applied intravaginally. These results are significant considering that very low doses of E2were used when compared to previous attempts. Despite the inadequate response to treatments in some of the gilts, the local effects of these hormones on the histological properties of the porcine cervix suggest that further improvements in the vaginal administration route might help to elaborate new methods for enhancing the luteal function in the pig.
Using of immunocytochemistry in differential diagnosis of neoplasms of serosal cavities in dogs
Authors: Przeździecki R. , Sapierzyński R. 
Published: September 2014

Abstract
The presence of tumor within the serosal cavities, often connected with accumulation of serosal effusion, is a quite common problem in the small animal veterinary medicine. The first step in diagnosis of such cases is cytopathological examination. The aim of the present study was to evaluate the usefulness of cytology and immunocytochemistry, using commercially available antibodies (anti-cytokeratin, anti-vimentin, and anti-desmin), in differential diagnosis of malignant tumors located within serosal cavities in dogs. The final cytological diagnosis of carcinoma/adenocarcinoma, sarcoma, and mesothelioma was obtained on the basis of routine cytopathology and immunocytochemistry, and then confirmed by histopathology and immunohistochemistry. Cytoplasmic immunoreactivitiy of normal mesothelid cells and cytoplasmic immunoreactivity of hyperplastic mesothelial cells revealed constant and strong expression of all examined intermediate filaments: cytokeratin, vimentin and desmin. Application of routine cytopathology and immunocytochemistry allowed 32 neoplastic tumors to be detected: 19 cases of carcinomas/adenocarcinomas, 6 cases of sarcomas, 7 cases of mesotheliomas. Immunostaining of cytopathological samples with chosen set of antibodies: anti-cytokeratin, anti-vimentin, anti-desmin is a useful, and low invasive test for differentiation between mesotheliomas and carcinomas/adenocarcinomas in dogs.
Effect of age and breeding season on sperm acrosin activity in the arctic fox (Alopex lagopus L.)
Authors: Stasiak K. , Janicki B. 
Published: September 2014

Abstract
The objective of this study was to determine the effect of age and reproductive season on selected properties of semen from the arctic fox, Alopex lagopus L. The experiment used 40 ejaculates collected manually from 6 animals (3 foxes aged one year and 3 foxes older than three years). Statistically less semen (0.39 cm3) was collected from the young compared to the older animals, and the ejaculates obtained were characterized by higher concentration of spermatozoa (195.04 x 106/cm3). In turn, sperm acrosomal extracts from the older animals contained statistically more acrosin (6,4 mU/106 spermatozoa). In the sperm acrosomal extracts prepared during the first semen sampling, the mean acrosin activity did not exceed 2.3 mU/million spermatozoa. At subsequent semen sampling dates, the activity of the analysed enzyme increased to reach 7.72mU/million spermatozoa. In the extracts obtained from the semen collected at the end of the breeding season of arctic foxes, the acrosin activity again reached a value obtained at the beginning of the season.
Post-harvest Salmonella spp. prevalence in turkey carcasses in processing plant in the northeast part of Poland
Authors: Zdrodowska B. , Liedtke K. , Radkowski M. 
Published: September 2014

Abstract
Turkeys carcasses at selected point after slaughter on dressing line in poultry were sampled and analyzed for Salmonella. These slaughter turkeys came from the northeast part of Poland. The examinations were carried out in each month of 2009. Three hundred turkeys were selected at random from a commercial slaughter line, immediately after completing the cooling process. The percentage of these 300 turkeys from which Salmonella spp. were isolated was relatively high (8.3%; Salmonella positive results were observed in 25 cases). The lowest Salmonella spp. rate (1.3 %) for slaughter birds was found in the fourth quarter, and the highest contamination rate at 18.6% was found, in the third quarter. The serological types of Salmonella spp. isolated from the whole turkey carcasses were S. Saintpaul, S. Senftenberg, S. Anatum, S. Heidelberg, S. Hadar, S. Typhimurium and S. Infantis.
Pharmacological characteristics of metamizole
Authors: Jasiecka A. , Maślanka T. , Jaroszewski J.J. 
Published: September 2014

Abstract
Metamizole (dipyrone) is a popular analgetic, non-opioid drug, commonly used in human and veterinary medicine. In some cases, this agent is still incorrectly classified as a non-steroidal anti-inflammatory drug (NSAID). Metamizole is a pro-drug, which spontaneously breaks down after oral administration to structurally related pyrazolone compounds. Apart from its analgesic effect, the medication is an antipyretic and spasmolytic agent. The mechanism responsible for the analgesic effect is a complex one, and most probably rests on the inhibition of a central cyclooxygenase-3 and activation of the opioidergic system and cannabinoid system. Metamizole can block both PG-dependent and PG-independent pathways of fever induced by LPS, which suggests that this drug has a profile of antipyretic action distinctly different from that of NSAIDs. The mechanism responsible for the spasmolytic effect of metamizole is associated with the inhibited release of intracellular Ca2+ as a result of the reduced synthesis of inositol phosphate. Metamizole is predominantly applied in the therapy of pain of different etiology, of spastic conditions, especially affecting the digestive tract, and of fever refractory to other treatments. Co-administration of morphine and metamizole produces superadditive, antinociceptive effects. Metamizole is a relatively safe pharmaceutical preparation although it is not completely free from undesirable effects. Among these side-effects, the most serious one that raises most controversy is the myelotoxic effect. It seems that in the past the risk of metamizole- induced agranulocytosis was exaggerated. Despite the evidence showing no risk of teratogenic and embryotoxic effects, the drug must not be administered to pregnant women, although it is allowed to be given to pregnant and lactating animals. This paper seeks to describe the characteristics of metamizole in the light of current knowledge.
Blood-brain barrier permeability imaging using perfusion computed tomography
Authors: Avsenik Jernej , Bisdas Sotirios , Popovic Katarina Surlan 
Published: September 2014

Abstract
Background. The blood-brain barrier represents the selective diffusion barrier at the level of the cerebral microvascular endothelium. Other functions of blood-brain barrier include transport, signaling and osmoregulation. Endothelial cells interact with surrounding astrocytes, pericytes and neurons. These interactions are crucial to the development, structural integrity and function of the cerebral microvascular endothelium. Dysfunctional blood-brain barrier has been associated with pathologies such as acute stroke, tumors, inflammatory and neurodegenerative diseases.
Conclusions. Blood-brain barrier permeability can be evaluated in vivo by perfusion computed tomography - an efficient diagnostic method that involves the sequential acquisition of tomographic images during the intravenous administration of iodinated contrast material. The major clinical applications of perfusion computed tomography are in acute stroke and in brain tumor imaging.
Three-dimensional analysis of cavity wall deformation after composite restoration of masticatory teeth
Authors: Manchorova-Veleva Neshka А. 
Published: September 2014

ABSTRACT
AIM: The aim of the present work was to study the size of cavity wall deformation in eight class I and II defects after composite restoration.
MATERIALS AND METHODS: 1. Creating a geometric model - data on the size of the left maxillary second premolar were obtained from a routine craniofacial scanning of a 20-yearold patient with a 2,5 Dental CT scanner (General Electric), with high resolution and 0.625mm-thin slices. The contour of each of the 33 cross-sections of tooth 25 was delineated using graphics software (CorelDraw 7.0) and transferred to a specialized product for engineering design (SolidWorks Offi ce Premium 2010, SolidWorks Corp. USA). The pulp cavity and periodontal ligament were created in the same manner and were integrated in the premolar body; 2. Generation of a fi nite element method - the geometric model was exported
to specialized software for analysis by the fi nite element method - COSMOSWorks 2010, which automatically builds a 3D fi nite elements mesh. Based on the generated model, eight additional models of class I and II cavities with different geometries, adhesive layer and nanofi lled composite restorations were constructed. The polymerization shrinkage was modelled by thermal deformation, with a negative temperature difference (cooling), corresponding to the actual volume shrinkage of the composite materials by 2.1%.
RESULTS: In models A and B, the maximum cavity wall displacement was small - 0.014 mm and 0.015 mm, respectively. In models A1, B1, C1 and C, the displacement was at the expense of large deformation of the dental tissues. The maximum cavity wall displacements were 0.020 mm, 0.026 mm, 0.020 mm, 0.035 mm, respectively. The least cavity wall displacement was in models A2 and B2 with 0.008 mm and 0.017 mm, respectively.
CONCLUSIONS: The least displacement resulting from cavity wall deformation is found in patient-friendly class I and II preparations. Preservation of the dental tissues reduces the risk of mechanical pressure on the dentinal lymph and the likelihood of post-operative sensitivity.
Effect of Biocompatible Peritoneal Dialysis Solution on Residual Renal Function: A Systematic Review of Randomized Controlled Trials
Authors: Seo Eun-Young , An Sook Hee , Cho Jang-Hee , Suh Hae Sun , Park Sun-Hee , Gwak Hyesun , Kim Yong-Lim , Ha Hunjoo 
Published: September 2014

Introduction Residual renal function (RRF) plays an important role in outcome of peritoneal dialysis (PD) including mortality. It is, therefore, important to provide a strategy for the preservation of RRF. The objective of this study was to evaluate relative protective effects of new glucose-based multicompartmental PD solution (PDS), which is well known to be more biocompatible than glucose-based conventional PDS, on RRF compared to conventional PDS by performing a systematic review (SR) of randomized controlled trials. Methods We searched studies presented up to January 2014 in MEDLINE, EMBASE, the COCHRANE library, and local databases. Three independent reviewers reviewed and extracted prespecified data from each study. The random effects model, a more conservative analysis model, was used to combine trials and to perform stratified analyses based on the duration of follow-up. Study quality was assessed using the Cochrane Handbook for risk of bias. Eleven articles with 1,034 patients were identified for the SR. Results The heterogeneity of the studies under 12 months was very high, and the heterogeneity decreased substantially when we stratified studies by the duration of follow-up. The mean difference of the studies after 12 months was 0.46 mL/min/1.73 m2 (95% confidence interval = 0.25 to + 0.67). Conclusion New PDS showed the effect to preserve and improve RRF for long-term use compared to conventional PDS, even though it did not show a significant difference to preserve RRF for short-term use.
Association of Pulmonary Hypertension with Mortality in Incident Peritoneal Dialysis Patients
Authors: Xu Qingdong , Xiong Liping , Fan Li , Xu Fenghua , Yang Yan , Li Huiyan , Peng Xuan , Cao Shirong , Zheng Zhihua , Yang Xiao , Yu Xueqing , Mao Haiping 
Published: September 2014

Background The prognostic value of pulmonary hypertension at the start of peritoneal dialysis (PD) in patient survival is unclear. Methods We conducted a retrospective study of incident patients who initiated PD therapy from January 2007 to December 2011, and followed up through June 2013. Pulmonary hypertension was defined as an estimated systolic pulmonary artery pressure (PAP) of ≥ 35 mm Hg using echocardiography. Clinical parameters and laboratory findings were compared between patients with and without pulmonary hypertension and a logistic regression model was elaborated. Patient outcomes (all-cause and cardiovascular mortality) were recorded during follow-up. Survival curves were constructed by the Kaplan-Meier method, and the influences of pulmonary hypertension on outcomes were analyzed by Cox regression models. Results Pulmonary hypertension was prevalent in 99 (16.0%) of the 618 patients studied. The independent risk factors for pulmonary hypertension were female (odds ratio [OR] = 2.12; 95% confidence interval [CI]: 1.29 – 3.46), left atrial diameter (OR = 1.15; 95% CI: 1.10 – 1.20), left ventricular ejection fraction (OR = 0.97; 95% CI: 0.95 – 0.99), and serum sodium (OR = 0.94; 95% CI: 0.89 – 0.99). Over a median follow-up of 29.4 months, 93 patients (15.0%) died, 59.1% of them due to cardiovascular disease. Kaplan-Meier survival analysis showed that patients with pulmonary hypertension had worse overall rates of survival and cardiovascular death-free survival than those without pulmonary hypertension. After multivariate adjustment, pulmonary hypertension was independently associated with increased risk for both all-cause and cardiovascular mortality, with hazard ratios (HRs) of 2.10 (95% CI: 1.35 – 3.27) and 2.60 (95% CI: 1.48 – 4.56), respectively. Conclusions The prevalence of pulmonary hypertension at the start of PD was common and associated with increased risk of both all-cause and cardiovascular mortality in incident PD patients.
Baseline Fgf23 is Associated with Cardiovascular Outcome in Incident Pd Patients
Authors: Kim Hyo Jin , Park Miseon , Park Hayne Cho , Jeong Jong Cheol , Kim Dong Ki , Joo Kwon Wook , Hwang Young-Hwan , Yang Jaeseok , Ahn Curie , Oh Kook-Hwan 
Published: September 2014

♦ Background Fibroblast growth factor 23 (FGF23) is a phosphate regulating protein. Several studies demonstrated that elevated FGF23 is independently associated with mortality for early-stage chronic kidney disease and incident hemodialysis (HD) patients. However, little is known about the significance of elevated FGF23 in peritoneal dialysis (PD) patients. Here, we analyzed the association of FGF23 with cardiovascular (CV) events, all-cause mortality, residual renal function (RRF), and CV parameters in PD patients. ♦ Methods The present study is a single-center, retrospective study. Patients who started PD at Seoul National University Hospital between January 2005 and July 2011 and whose baseline serum samples were available were enrolled. C-terminal FGF23 was measured. Subjects were divided into 2 groups; lower 2 tertiles (FGF23 ≤ 119.0 RU/mL) and top tertile (FGF23 > 119.0 RU/mL). The primary outcome was time to fatal or non-fatal CV events. In the subgroup analysis, the associations of FGF23 with aortic stiffness or with vascular calcification were analyzed. ♦Results A total of 205 incident PD patients were analyzed. Mean duration of follow-up was 41.6 ± 20.0 months. The baseline median FGF23 level was 78.6 RU/mL (interquartile range [IQR], 34.1 – 155.0). At baseline, subjects in the higher FGF23 group were younger, and had a lower RRF, lower prevalence of diabetes mellitus (DM), and cerebrovascular disease. During follow-up, 22 of the 205 patients (10.7%) reached primary outcome. After adjustment for age, DM, pre-existing coronary artery disease, cerebrovascular disease, congestive heart failure, and left ventricular mass index, the higher FGF23 group exhibited significantly higher risk of primary outcome, compared with the lower group (hazard ratio [HR], 2.54; 95% confidence interval [CI], 1.05 – 6.12; p = 0.045). There were no significant differences in all-cause mortality and development of anuria between the 2 FGF23 groups. In the subgroup analysis, FGF23 groups were not associated with pulse wave velocity and abdominal aortic calcification score. ♦ Conclusion Elevated FGF23 is associated with higher risk of adverse CV outcome for incident PD patients.
Contraindre et habiliter : la double dimension des outils de contrôle
Authors: Ragaigne Aurélien , Oiry Ewan , Grimand Amaury 
Published: September 2014

Les travaux de Michel Foucault ont fondé un courant de recherche qui a mis en évidence les mécanismes permettant aux dispositifs de contrôle de contraindre les individus. Le lien entre savoir et pouvoir joue un rôle majeur dans cette dynamique. Cet article propose d’enrichir ces travaux avec ceux d’Armand Hatchuel qui a prolongé la réflexion que Michel Foucault a réalisée sur ce lien. Les savoirs ont en effet, une double facette : ils contraignent mais, par leur dimension réflexive, ils habilitent aussi les salariés. Les savoirs leur permettent de développer de nouveaux comportements qu’ils ont eux-mêmes définis. Sur cette base conceptuelle, notre recherche souligne que les outils de contrôle ont simultanément cette double dimension de contrainte et d’habilitation. L’étude de ces dimensions apparaît ainsi comme une clé particulièrement importante pour l’analyse des usages des outils de contrôle. Dans cet article, nous présentons deux études de cas mettant en évidence cette double dimension d’habilitation et de contrainte. Les données collectées ont été obtenues à partir d’entretiens semi-directifs et d’études documentaires approfondies. La première étude de cas montre qu’un outil de contrôle des compétences initialement conçu dans une logique d’habilitation peut être utilisé dans une optique plus contraignante. Inversement, la deuxième étude de cas montre qu’une enquête de satisfaction, un outil initialement plutôt orienté vers la contrainte, peut aussi habiliter les salariés. Dans la discussion, ces résultats empiriques sont utilisés pour enrichir la littérature existante sur ls’articulation des dimensions habilitante et contraignante des outils de contrôle.