bluuebunny/crossref_metadata_2025_split huggingface dataset:
GERPACYST- The Trial Protocol Of The Prospective, Multicenter, Interdisciplinary German Pancreas Club Cyst Registry
Authors: Honselmann Kim Christin , Marschner Jonathan , Staufenbiel Anna , Bertram Julia , Deichmann Steffen , Engelke Carsten , Kirstein Martha , Marquardt Jens , Damm Marko , Borowitzka Fanny , Phillip Veit , Pergolini Ilaria , Harder Felix , Braren Rickmer , Gemoll Timo , Roth Susanne , Krug Sebastian , Michalski Christoph W. , Jaster Robert , Keck Tobias , Wellner Ulrich Friedrich ,   the GERPACYST Study Group
Published: August 2024

AbstractBackgroundCystic lesions of the pancreas have continued to present a clinical challenge for the past decades now. The increasing rate of detection, the lack of high-quality data on the natural biology of pancreatic cysts and the resulting difficulty to predict malignant transformation in different types of pancreatic cysts make patients with these diseases hard to manage. The German Pancreas Club Cyst Registry (GERPACYST) (DRKS00025927) establishes a platform to discover the natural entity specific biology of pancreatic cysts, in a multicenter manner that should allow to assess risk models for malignancy.MethodThis manuscript is written according to the SPIRIT guidelines. Ethical approval was obtained from the University of Luebeck (2024-265_1). In GERPACYST patients aged ≥18 years with a pancreatic cyst under surveillance or scheduled for surgery should be enrolled. Participating centers will complete an electronic Case Report Form (eCRF) via REDCap which is designed as a longitudinal study minimizing the input of repeated measures. Changes in patient baseline data, cyst characteristics, both endoscopic and imaging data will be entered typically every 6-12 months during patient follow-up. Biobanking will be performed, when available. Duration of observation per patient is up to a maximum of 20 years or until end of follow-up or death.DiscussionGERPACYST will provide a valuable platform for clinical outcomes research. Fundamental factors affecting the development of pancreatic cysts over time will be identified. New research questions might be answered during the study period.Trial registrationThe study was prospectively registered at the German Clinical Trial Register (DRKS) under DRKS00025927 on September, 14th, 2021 before inclusion of the first patient. The Universal Trial Number (UTN) is U1111-1302-9822.Trial sponsorThe study is sponsored by the German Pancreas Club (DPC) e.V. The sponsor has no role in study design, collection, management, interpretation or writing of the report. It is a mere supportive sponsoring.
Cardiothoracic Ratio (CTR) Among Patients Presenting for Chest X-ray in Radiology Department at Mulago National Referral Hospital: A Patients’ Health Indicator for Clinical Application
Authors: Musisi Alen , Nakatudde Rebecca , Namuwonge Oliver , Babirye Deborah , Kintu Ismail , Olweny Francis , Malumba Richard , Nakalanzi Victoria , Mubuuke Aloysius Gonzaga 
Published: August 2024

ABSTRACTIntroduction/backgroundThe heart is vital, and even minor dysfunctions can significantly impact the body. Cardiologists need always to determine heart size, which varies with physiological changes. Advanced measurement techniques are costly and often inaccessible to a common man. Measuring the cardiothoracic ratio (CTR) via conventional X-ray is a common and more affordable option, but there’s a need for even cheaper alternativesObjectiveTo determine relationship between CTR and presenting clinical indications and to relate CTR to the body parameters to find an appropriate relationship that can be utilized in low resource facilities in determining heart size.MethodologyThis cross-sectional study involved 386 patients undergoing chest radiographs at Mulago National Specialized Hospital’s radiology department. Data were summarized using frequencies and percentages. Associations between the cardiothoracic ratio (CTR) and independent variables were analyzed using Pearson’s chi-square, Fisher’s exact test, Spearman’s correlation coefficient, simple linear regression, and multivariate regression. Statistical significance was set at a p-value of < 0.05.ResultsThe median cardiothoracic ratio (CTR) was 0.46, with an interquartile range of 0.42 to 0.50. Female patients had a higher CTR than males. Significant positive correlations were found between CTR; and BMI (p < 0.001, correlation 0.21), and BSA (p = 0.016, correlation 0.12), and BSI (p < 0.001, correlation 0.19). The diagnostic accuracy of a linear regression equation containing BSA as an estimator of CTR showed relatively fair performance compared to the linear regression equations with BSI and BMI. It showed sensitivity, specificity, and positive and negative predictive values of 29.2%, 86.0%, 63.6%, and 59.0% for males, and 8.3%, 98.1%, 75.0%, and 60.7% for females, respectively.ConclusionBSA shows a moderately good relationship with CTR, while the influence of body habitus on CTR is minimal. Thus, using body parameters to predict CTR should be approached cautiously. We recommend conducting a similar study on a more diverse general population
Efficacy and safety of tirofiban after intravenous tenecteplase for acute ischemic stroke: the multicenter, randomized, placebo-controlled, double-blind, INSTANT trial protocol
Authors: Zhang Fan , Xiao Minghui , Zhang Cong , Yuan Guangxiong , Xie Zhiyong , Yin Yi , Zhou Ruize , Yuan Shanggui , Xiao Genxiang , Mei Donghuan , Zeng Xiaobing , Liu Huashi , Li Huadong , Liu Hongwen , Tan Jinchang , Chen Bin , Fu Qingqing , Li Bin , Lai Jinxing , Sun Wei , Xie Shuhua , Lai Zhaohui , Qiu Zhongming , Jiang Zidian , Liu Xianghong , Zeng Guoyong 
Published: August 2024

AbstractBackgroundIt is very common that symptoms do not improve or even worsen after intravenous thrombolysis (IVT) in acute ischemic stroke. However, it remains unknown whether early administration of tirofiban after IVT may improve clinical outcomes.ObjectiveThis trial aims to assess the efficacy and safety of early administration of tirofiban after intravenous thrombolysis with Tenecteplase in acute ischemic stroke within 24 hours of symptom onset.Methods and designThe INSTANT trial is an investigator-initiated, randomized, placebo-controlled, double-blind, multicenter trial. Up to 310 patients with acute non-large/medium-sized vascular occlusion and non-atrial fibrillation stroke who are treated with intravenous tenecteplase will be consecutively randomized to intravenous tirofiban or placebo in a 1:1 ratio over two years across 50 intravenous thrombolysis-capable stroke centers in China. Subjects will be treated with intravenous tirofiban or placebo at a dose of 0.3 μg per kilogram of body weight per minute for 30 minutes, followed by a continuous infusion of 0.075 μg per kilogram per minute for up to 47.5 hours.OutcomesThe primary efficacy outcome is the proportion of patients with excellent functional outcomes (defined as a score of 0-1 on the modified Rankin scale (mRS)) at 90 days. The secondary outcome is the proportion of patients achieving functional independence, defined as a mRS score of 0-2 at 90 days. Safety outcomes include symptomatic intracranial hemorrhage within 48 hours and mortality at 90 days.DiscussionThis randomized, double-blind, placebo-controlled trial will provide data regarding the role of early antiplatelet therapy with intravenous tirofiban after IVT.Trial registry numberChiCTR2300074368 (www.chictr.org.cn).
Strigolactone receptor DAD2 promotes lateral root formation inPetunia hybrida
Authors: Khuvung Khopeno , Courty Pierre-Emmanuel , Reinhardt Didier 
Published: August 2024

AbstractStrigolactone regulates various aspects of plant development and interactions with beneficial microbes. While the role of SL in shoot development is highly conserved between monocots and dicots, its role in root system architecture is controversial and context-dependent, although most of the SL appears to be produced there. A central parameter in plant fitness is root-to-shoot ratio, which is determined by growth and branching activity of both, the root as well as the shoot. We used previously characterized mutants defective in SL biosynthesis and sensing to test the role of SL in root growth and branching. Here, we show that a mutant defective in the petunia strigolactone receptor (dad2) exhibits defects in root meristem activity and root branching. Notably, this phenotype is not observed in the SL biosynthetic mutantdad1, suggesting that DAD2 may perceive a strigolactone analogue that is produced in a DAD1-independently fashion. DAD2 is induced in cortex cells adjacent to lateral root primordia, where it allows them to penetrate the cortex and to grow out of the main root. We propose a model by which inducible SL perception in cells adjacent to LRPs promotes auxin-induced cell wall remodeling in the cortex to allow passage of lateral roots.1-sentence summaryLateral roots are initiated inside the main root, hence, they have to penetrate several cell layers to grow out. We propose that strigolactone cooperates with auxin to mediate lateral root emergence.
High-Throughput Assay for Predicting Diarrhea Risk Using a 2D Human Intestinal Stem Cell-Derived Model
Authors: Pike Colleen M , Levi James A , Boone Lauren A , Peddibhotla Swetha , Johnson Jacob , Zwarycz Bailey , Bunger Maureen K , Thelin William , Boazak Elizabeth M 
Published: August 2024

AbstractGastrointestinal toxicities (GITs) are the most prevalent adverse events (AE) reported in clinical trials, often resulting in dose-limitations that reduce drug efficacy and delay development and treatment optimization. Preclinical animal models do not accurately replicate human GI physiology, leaving few options for early detection of GI side effects prior to human studies. Development of an accurate model that predicts GIT earlier in drug discovery programs would better support successful clinical trial outcomes. Chemotherapeutics, which exhibit high rates of clinical GIT, frequently target mitotic cells. Therefore, we hypothesized that a model utilizing proliferative cell populations derived from human intestinal crypts would predict the occurrence of clinical GITs with high accuracy. Here, we describe the development of a multiparametric assay utilizing the RepliGut® Planar system, an intestinal stem cell-derived platform cultured in an accessible high throughput Transwell™ format. This assay addresses key physiological elements of GIT by assessing cell proliferation (EdU incorporation), cell abundance (DAPI quantification), and barrier function (TEER). Using this approach, we demonstrate that primary proliferative cell populations reproducibly respond to marketed chemotherapeutics at physiologic concentrations. To determine the ability of this model to predict clinical diarrhea risk, we evaluated a set of 30 drugs with known clinical diarrhea incidence in three human donors, comparing results to known plasma drug concentrations. This resulted in highly accurate predictions of diarrhea potential for each endpoint (balanced accuracy of 91% for DAPI, 90% for EdU, 88% for TEER) with minimal variation across human donors. In vitro toxicity screening using primary proliferative cells may enable improved safety evaluations, reducing the risk of AEs in clinical trials and ultimately lead to safer and more effective treatments for patients.
The Y951N patient mutation inactivates the intramolecular switch in human mitochondrial DNA POLγ
Authors: Forslund Josefin M. E. , Nguyen Tran V.H. , Parkash Vimal , Berner Andreas , Goffart Steffi , Pohjoismäki Jaakko L.O. , Wanrooij Paulina , Johansson Erik , Wanrooij Sjoerd 
Published: August 2024

AbstractMitochondrial DNA (mtDNA) stability, essential for cellular energy production, relies on DNA polymerase gamma (POLγ). Here, we show that the POLγ Y951N disease causing mutation induces replication stalling and severe mtDNA depletion. However, unlike other POLγ disease causing mutations, Y951N does not directly impair exonuclease activity and only mildly affects polymerase activity. Instead, we found that Y951N compromises the enzyme’s ability to efficiently toggle between DNA synthesis and degradation, and is thus the first patient-derived mutation with impaired polymerase-exonuclease switching. These findings provide new insights into the intramolecular switch when POLγ proofreads the newly-synthesized DNA strand, and reveal a new mechanism for causing mitochondrial DNA instability.Significance StatementDNA polymerase gamma (POLγ) is essential for copying mitochondrial DNA (mtDNA), which is crucial for our energy production. POLγ must accurately switch between making new DNA (polymerase activity) and correcting errors (exonuclease activity). While it is known that mutations in POLγ can cause mitochondrial diseases by directly impairing these enzymatic functions, this study reveals a new mechanism. The Y951N mutation disrupts POLγ’s ability to switch between these activities, leading to severe blockages in DNA replication and a loss of mtDNA in human cells, even without significant direct impairment of polymerase or exonuclease activities. These findings provide new insights into the origins of mitochondrial diseases.
Sex specific knee joint soft tissue mineralization with Fibrillin-1 mutation in male Tight Skin mice
Authors: Keenan Craig , Wang Xi , Dikmen Tayfun , Wen Yan , Ramos-Mucci Lorenzo , Shorter Emily , Abraham David , Bou-Gharios George , Poulet Blandine 
Published: August 2024

AbstractArticular soft tissue mineralization and ossification are clear pathological signs of osteoarthritis joints. However their molecular and cellular aetiologies remain largely unknown. TGF-ß family members are known contributors to both pathological ossification and osteoarthritis development. In this study, we used a Fibrillin-1 mutant mouse, the Tight Skin mouse (TSK), to define the detrimental effects of abnormal Fbn1 in TSK mice and known high TGF-ß activity in joint pathology such as articular soft tissue mineralization and ossification.Knee joints of male and Female TSK and Wild-type (WT) littermates were analysed my micro-CT imaging and histology for articular soft tissue pathologies, as well as OA severity. Both aged (10, 26, 35 and 52wks) and following in vivo non-invasive repetitive joint overloading were used.We find that male TSK mice develop spontaneous soft tissue ossification from 26wks of age, followed by increased osteoarthritis at 1 year-old. In addition, knee joint overloading induced ligament and meniscal mineralisation and ossification in both WT and TSK male mice, but were significantly more severe in TSK knees, including ossification of the patella ligament and synovial lining. In contrast, female TSK knees did not develop more severe soft tissue mineralisation compared to littermate WT mice in neither aged nor overloaded knees.We conclude that Fbn1 mutation, and possibly overactive TGF-ß activity in TSK mice, induce articular soft tissue ossification and osteoarthritis in a sex-specific manner. Further studies are needed to confirm the specific signalling involved and the relative protection from female mice from such pathologies.
Study of quantum decoherence at the Protvino to ORCA experiment
Authors: Bera Chinmay , Deepthi K. N. 
Published: August 2024

Protvino to ORCA (oscillation research with cosmics in the abyss) (P2O) is an upcoming neutrino oscillation experiment with a very long baseline of 2595 km. Because of the substantial baseline, this experiment provides a unique opportunity to study the Earth matter effects over very large distances. This makes it a suitable experiment to investigate the environmental decoherence in neutrino oscillations, where the neutrino system could interact with a stochastic environment and lead to a loss in the coherence of neutrino states. In this work, we consider an open quantum system framework to simulate the neutrino oscillations in the P2O experiment and obtain bounds on the decoherence parameters in different phenomenological models. Here, we also consider that the decoherence parameter Γ depends on neutrino energy Eν as Γjk(Eν)=Γ0(EνE0)n. Furthermore, assuming the presence of decoherence in nature, we study the effect of it on the determination of neutrino mass hierarchy and CP violation at the P2O experiment.

Published by the American Physical Society
2024
More than just a family affair? The preventive effect of privatized CEO communication on the perception of an organization in crisis
Authors: Denner Nora , Viererbl Benno , Weismantel Maike 
Published: August 2024

PurposeThis study examines the role of CEO communication in effective crisis management. Specifically, it examines whether the communication of private information about a CEO can create a positive pre-crisis image that serves as a buffer during actual crises.Design/methodology/approachThis study uses a 2x2 experimental design to analyze the effect of CEO communication (private information vs no private information) on pre-crisis image and its effectiveness in different types of crises (victim crisis vs preventable crisis).FindingsThe results of this research show that the communication of private information about a CEO contributes to the improvement of public image perceptions when a crisis occurs. This effect is influenced by the recipient’s identification with the organization as well as perceptions of empathy and competence toward the CEO. Notably, stronger effects are observed in the context of a victim crisis.Originality/valueThis study contributes to the field by highlighting the importance of CEO communication in crisis management and its potential to proactively build a positive pre-crisis image. In addition, it examines how this mechanism varies by crisis type, providing valuable insights for crisis communication strategies.
Service failure and negative Word-of-Mouth in Chinese retail banking: a moderated-mediation approach
Authors: Zhao Cong , Noman Abu Hanifa Md. , Abedin Mohammad Zoynul 
Published: August 2024

PurposeAs opposed to conventional promotional methods, Word-of-Mouth (WOM) communication, especially when negative, significantly shapes customers’ repurchase decisions and preferences. Therefore, this study aims to examine the interplay between negative WOM and bank service failures, with a focus on the mediating role of customer switching intentions and the moderating role of switching costs in this relationship.Design/methodology/approachUsing an online semi-structured questionnaire survey, a dataset comprising 411 responses was gathered from retail bank customers in China. This dataset was subsequently analyzed using SPSS PROCESS.FindingsConsistent with the social exchange theory, our study revealed a significant relationship between service failure and both bank customers’ intention to switch and negative WOM communication. Additionally, we observed that switching intentions significantly influence negative WOM communications, acting as a mediator between service failures and negative WOM. Furthermore, our findings indicated that switching costs moderate the direct effect of service failures on negative WOM and moderate the indirect effect of service failures on negative WOM through switching intentions.Research limitations/implicationsThis study provides significant policy implications aimed at minimizing bank service failures and subsequent negative WOM communications among bank customers.Originality/valueThis study empirically investigates the role of service failures in promoting negative WOM communication, demonstrating a partial mediation effect of switching intentions in this relationship. Moreover, the study highlights that switching costs moderate service failures’ impact on customers’ switching intentions.
Teachers’ understandings of the anger they feel toward their principals: a phenomenographic study
Authors: Özaslan Gökhan , Karakuzu Ölemez Sümeyranur Meryem , Polat Sultan , Balıkcı İlker Berat , Uyanık Mustafa 
Published: August 2024

PurposeThe purpose of this study is to reveal the different ways in which a group of teachers understand the anger they feel toward the school principals with whom they interact.Design/methodology/approachThe present study was designed and conducted as a phenomenography. In phenomenographic research, the number of different ways in which participants understand a particular phenomenon is revealed. The data from semi-structured interviews with 15 teachers who differed in terms of school level, gender and age were analyzed according to the phenomenographic tradition.FindingsThe analysis revealed that although the participants had eight different understandings of the anger in question, all participants had an understanding that was described as “An emotion arising from not being treated with the respect we deserve.”Practical implicationsThe source of anger for the participating teachers was the behavior of their principals, which made them feel that they were not considered respectable and valuable. Incorporating this insight into the training of school leaders may lead to useful outcomes in improving the quality of interactions between principals and teachers.Originality/valueAs one of the few examples of phenomenographic research in educational management, the present study contributes to providing a solid foundation for exploring the anger that teachers feel toward their principals by showing how and in what ways participating teachers may understand this feeling.
Effect of smart tourism technology experience (STTE) on tourist satisfaction and tourist happiness in Bordeaux: the mediation role of self-gratification
Authors: Yuksel Faruk , Kement Uzeyir , Dogan Seden , Erkol Bayram Gul , Bayar Sinan Baran , Cobanoglu Cihan 
Published: August 2024

Purpose
This study aims to investigate the effects of smart tourism technology experience (STTE) on tourist satisfaction and happiness in Bordeaux, with a focus on understanding the mediating role of self-gratification. By examining these relationships, the study seeks to provide insights into how smart tourism technologies can enhance tourist experiences.

Design/methodology/approach
The study uses partial least squares-structural equation modeling (PLS-SEM) to analyze data collected from 380 tourists who visited Bordeaux. The measurement model assesses reliability and validity, while the structural model evaluates the proposed hypotheses and the mediation effects of self-gratification.

Findings
The results confirm that STTE positively impacts tourist satisfaction, with accessibility, informativeness and personalization significantly enhancing tourist satisfaction, while interactivity does not. Tourist satisfaction, in turn, positively affects tourist happiness. Furthermore, self-gratification partially mediates the relationship between tourist satisfaction and happiness, highlighting its importance in the smart tourism context.

Originality/value
This research extends the understanding of STTE by demonstrating its effects on tourist satisfaction and happiness. It introduces the mediating role of self-gratification, providing a novel perspective on how personalized smart tourism experiences contribute to overall tourist happiness.