bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison 30-day clinical complications between transfemoral versus transapical aortic valve replacement for aortic stenosis: a meta-analysis review
Authors: Li Xuebiao , Kong Minjian , Jiang Daming , Dong Aiqiang 
Published: July 2013

AbstractBackgroundSince 2002, transapical aortic valve replacement has been developed as a clinical pathway for transcatheter aortic valve implantation (TAVI). However the appropriate role of TA in the AS population versus TF remains unclear. We performed a meta-analysis to assess if TF has any benefit in reduction of 30-day clinical complications in AS.MethodsWe conducted a comprehensive search on pub-med and web of knowledge from 2002 through September 2012 using following terms: aortic stenosis, aortic valve replacement, transcatheter aortic valve implantation, TAVI, trans-artery, transfemoral, trans-apical. Studies in the original research or review articles were also considered. Included studies must meet the preconditioned criterias. Two investigators independently browsed the studies by title and abstract, finally making decision according to full-text. Disagreements were discussed in group.ResultsA total of 20 studies met inclusion criteria’s and were included in the analysis (including 4267 patients in TF group, 2242 in TA group). No random clinical trial, one was a retrospective study, others were prospective trials. Our meta-analysis found that TF had the low incidence of 30-day mortality compared with TA procedure (7.5% versus 11.3%). The incidence of stroke at ≤ 30 days was relatively low (3.8% in TF versus 4.0% in TA). Although the incidence of post-operative heart block was high (8.5% versus 7.5%), but no differences were indicated [1.06,95% CI(0.85,1.33)].ConclusionsThe result of our meta-analysis suggested that TF may have a low risk for 30-day mortality against TA procedure. No difference was found in the incidence of post-operative stroke and heart block.
GenePattern flow cytometry suite
Authors: Spidlen Josef , Barsky Aaron , Breuer Karin , Carr Peter , Nazaire Marc-Danie , Hill Barbara Allen , Qian Yu , Liefeld Ted , Reich Michael , Mesirov Jill P , Wilkinson Peter , Scheuermann Richard H , Sekaly Rafick-Pierre , Brinkman Ryan R 
Published: July 2013

AbstractBackgroundTraditional flow cytometry data analysis is largely based on interactive and time consuming analysis of series two dimensional representations of up to 20 dimensional data. Recent technological advances have increased the amount of data generated by the technology and outpaced the development of data analysis approaches. While there are advanced tools available, including many R/BioConductor packages, these are only accessible programmatically and therefore out of reach for most experimentalists. GenePattern is a powerful genomic analysis platform with over 200 tools for analysis of gene expression, proteomics, and other data. A web-based interface provides easy access to these tools and allows the creation of automated analysis pipelines enabling reproducible research.ResultsIn order to bring advanced flow cytometry data analysis tools to experimentalists without programmatic skills, we developed the GenePattern Flow Cytometry Suite. It contains 34 open source GenePattern flow cytometry modules covering methods from basic processing of flow cytometry standard (i.e., FCS) files to advanced algorithms for automated identification of cell populations, normalization and quality assessment. Internally, these modules leverage from functionality developed in R/BioConductor. Using the GenePattern web-based interface, they can be connected to build analytical pipelines.ConclusionsGenePattern Flow Cytometry Suite brings advanced flow cytometry data analysis capabilities to users with minimal computer skills. Functionality previously available only to skilled bioinformaticians is now easily accessible from a web browser.
Neutrophil activation by Campylobacter concisus
Authors: Sørensen Nina B , Nielsen Hans L , Varming Kim , Nielsen Henrik 
Published: July 2013

Abstract

Background

Campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. The magnitude of systemic immunoglobulin response in acute infection is unknown.

Methods
Neutrophils from healthy volunteers were activated with five faecal isolates of C. concisus from patients with gastroenteritis as well as the oral reference strain C. concisus ATCC33237. Neutrophils were tested for the expression of adherence molecule CD11b by immunoflourescence and for oxidative burst response by chemiluminescence. The opsonic activity in a chemiluminescence assay was assessed with heat treated serum from patients with C. concisus infection.

Results
A strong and dose-dependent activation of neutrophil adherence molecule CD11b and oxidative burst response was demonstrated with all six C. concisus isolates. Bacteria opsonised in heat treated serum induced an increased chemiluminescence response. Heat treated serum from patients with C. concisus infection did not have a higher opsonic activity than heat treated serum from healthy volunteers.

Conclusion

C. concisus has the capability to activate the innate immune system by stimulating neutrophil cells to increased adherence molecule expression and oxidative burst response, both crucial for acute inflammation. In a chemiluminescence assay the opsonic activity of heat treated serum from patients was not increased compared to heat treated control serum suggesting a weak systemic IgG response to infection.
The Putzig partners DREF, TRF2 and KEN are involved in the regulation of the Drosophila telomere retrotransposons, HeT-A and TART
Authors: Silva-Sousa Rute , Varela Míriam Díaz , Casacuberta Elena 
Published: July 2013

Abstract

Background
Telomere maintenance in Drosophila relies on the targeted transposition of three very special non-LTR retrotransposons, HeT-A, TART, and TAHRE (HTT). The sequences of the retrotransposon array build up the telomere chromatin in this organism. We have recently reported the role of the chromosomal protein Putzig/Z4 in maintaining a proper chromatin structure at the telomere domain of Drosophila. Because the Putzig protein has been found in different cellular complexes related with cell proliferation, development, and immunity, we decided to investigate whether the previously described Putzig partners, DREF/TRF2 and KEN, could also be involved in the telomere function in this organism.

Results
We have found that mutant alleles for Dref/Trf2 and Ken show alterations in HeT-A and TART expression, suggesting a possible role of these protein complexes in the regulation of the telomere retrotransposons. In agreement, both HeT-A and TART contain the specific DNA binding sequences for the DREF and the KEN protein proteins.

Conclusions
We have identified three new negative regulators involved in the control of the expression of the telomeric retrotransposons, Dref, Trf2, and Ken. Our results offer some clues on which other chromatin-related proteins might be involved in telomere regulation and retrotransposon control.
Severe everolimus-induced steatohepatis: a case report
Authors: Schieren Gisela , Bölke Edwin , Scherer Axel , Raffel Andreas , Gerber Peter Arne , Kröpil Patric , Schott Matthias , Hamilton Jackson , Hayman Anne , Knoefel Wolfram Trudo , Budach Wilfried , Matuschek Christiane 
Published: July 2013

Abstract
The mammalian target of rapamycin inhibitors are normally favored as immunosuppressant agents for solid organ transplantation such as kidney, liver or heart. Only in recent years have they been increasingly administered for the treatment of neuroendocrine tumors. Even though mammalian target of rapamycin inhibitors are known to exhibit specific side effects, everolimus-related severe hepatic steatosis has not as yet been described in the literature. We report the case of a 76-year-old man who developed severe hepatic steatosis within four weeks of treatment with everolimus as concomitant tumor therapy for a progressively growing neuroendocrine carcinoma of the ileum. A diagnosis of hepatic steatosis was established using computer tomography and fibroscan©. Other underlying causes for steatosis hepatis could be excluded. Further studies are warranted to explain the underlying mechanisms.
Anaerobic slurry co-digestion of poultry manure and straw: effect of organic loading and temperature
Authors: Babaee Azadeh , Shayegan Jalal , Roshani Anis 
Published: July 2013

Abstract
In order to obtain basic design criteria for anaerobic digestion of a mixture of poultry manure and wheat straw, the effects of different temperatures and organic loading rates on the biogas yield and methane contents were evaluated. Since poultry manure is a poor substrate, in term of the availability of the nutrients, external supplementation of carbon has to be regularly performed, in order to achieve a stable and efficient process. The complete-mix, pilot-scale digester with working volume of 70 L was used. The digestion operated at 25°C, 30°C and 35°C with organic loading rates of 1.0, 2.0, 2.5, 3.0, 3.5 and 4.0 kg Volatile solid/m3d and a HRT of 15 days. At a temperature of 35°C, the methane yield was increased by 43% compared to 25°C. Anaerobic co-digestion appeared feasible with a loading rate of 3.0 kg VS/m3d at 35°C. At this state, the specific methane yield was calculated about 0.12 m3/kg VS with a methane content of 53–70.2% in the biogas. The volatile solid (VS) removal was 72%. As a result of volatile fatty acid accumulation and decrease in pH, when the loading rate was less than 1 or greater than 4 kg VS/m3d, the process was inhibited or overloaded, respectively. Both the lower and higher loading rates resulted in a decline in the methane yield.
Excessive milk production during breast-feeding prior to breast cancer diagnosis is associated with increased risk for early events
Authors: Gustbée Emma , Anesten Charlotte , Markkula Andrea , Simonsson Maria , Rose Carsten , Ingvar Christian , Jernström Helena 
Published: July 2013

Abstract
Breast-feeding is a known protective factor against breast cancer. Breast-feeding duration is influenced by hormone levels, milk production, and lifestyle factors. The aims were to investigate how breast-feeding duration and milk production affected tumor characteristics and risk for early breast cancer events in primary breast cancer patients. Between 2002 and 2008, 634 breast cancer patients in Lund, Sweden, took part in an ongoing prospective cohort study. Data were extracted from questionnaires, pathology reports, and patients’ charts from 592 patients without preoperative treatment. Breast-feeding duration ≤12 months of the first child was associated with higher frequency of ER+/PgR+ tumors (P=0.02). Median follow-up time was 4.9 years. Higher risk for early events was observed for breast-feeding duration of first child >12 months (LogRank P=0.001), total breast-feeding duration >12 months (LogRank P=0.008), as well as ‘excessive milk production’ during breast-feeding of the first child (LogRank P=0.001). Patients with ‘almost no milk production’ had no events. In a multivariable model including both ‘excessive milk production’ and breast-feeding duration of the first child >12 months, both were associated with a two-fold risk for early events, adjusted HRs 2.33 (95% CI: 1.25-4.36) and 2.39 (0.97-5.85), respectively, while total breast-feeding duration was not. ‘Excessive milk production’ was associated with a two-fold risk of early distant metastases, adjusted HR 2.59 (1.13-5.94), but not duration. In conclusion, ‘excessive milk production’ during breast-feeding was associated with higher risk for early events independent of tumor characteristics, stressing the need to consider host factors in the evaluation of prognostic markers.
Succinate dehydrogenase expression in breast cancer
Authors: Kim Sewha , Kim Do Hee , Jung Woo-Hee , Koo Ja Seung 
Published: July 2013

Abstract
The aim of this study was to investigate succinate dehydrogenase (SDH) expression in breast cancer according to breast cancer molecular subtype using immunohistochemistry and to assess the clinical implications of SDH expression. Immunohistochemical staining for ER, PR, HER-2, Ki-67, HIF-1α, SDHA, and SDHB was performed on tissue microarrays of 721 breast cancers. According to the immunohistochemistry results for ER, PR, HER-2, and Ki-67 and fluorescence in situ hybridization (FISH) results for HER-2, breast cancer molecular subtypes were classified into luminal A, luminal B, HER-2, and triple-negative breast cancer (TNBC). HER-2 subtype breast cancers most frequently showed high-level expression of SDHA in tumor cells, while the luminal A subtype most frequently showed low or negative expression of SDHA in tumor cells (P = 0.032). Stromal SDHB expression rate was highest in HER-2 subtype and lowest in TNBC (P < 0.001). SDHA-negative breast cancers were associated with younger age at diagnosis (P = 0.012), and SDHB-negative breast cancers with lower histologic grade (P = 0.044) and lower Ki-67 labeling index (LI) (P = 0.046). Tumor phenotypes according to the SDH status were SDHA(+)/SDHB(+) > SDHA(–)/SDHB(–) > SDHA(–)/SDHB(+) > SDHA(+)/SDHB(–) in order of frequency. The stromal phenotypes were SDHA(–)/SDHB(–) > SDHA(+)/SDHB(+) > SDHA(–)/SDHB(+) > SDHA(+)/SDHB(–). In conclusion, loss of SDHA or SDHB expression was observed in about 3% of breast cancers in this study. Low SDH expression status in breast tumor cells was associated with younger age at diagnosis and low-grade histology.
Cohort profile: mental health following extreme trauma in a northern Ugandan cohort of W ar-A ffected Y outh S tudy (The WAYS Study)
Authors: Amone-P’Olak Kennedy , Jones Peter B , Abbott Rosemary , Meiser-Stedman Richard , Ovuga Emilio , Croudace Tim J 
Published: July 2013

AbstractWar experiences are associated with the risk of long-term mental health problems. The War-affected Youths (WAYS) Study comprises a cohort of 539 youths (61% male) aged between 18 to 25 (at baseline) randomly sampled from the population of war-affected youths in northern Uganda. The study aims to chart the trajectory of long-term mental health consequences of war and the roles of individual, family, and community contextual risk and protective factors in influencing the course of mental health using Social Ecology Model, thus, addressing both the individual and its social ecology. Knowledge of postwar contexts may inform policy and guide interventions on postwar psychosocial adjustment and reintegration in conflict-prone Great Lakes region of Africa (Rwanda, Burundi, DR Congo, Uganda, Central African Republic, and South Sudan). Two waves of data collection have been conducted and more data collection is planned. At baseline, information on demographic characteristics, pre-war experiences, psychosocial outcomes, coping, stigma/discrimination, family and community acceptance and relationship, family functioning, and post-war experiences were obtained. At follow-up, information on general health, gender-based violence, PTSD, social skills, trauma memory quality, rumination, self-esteem, and psychosocial outcomes were collected. Approval to access the data can be obtained on application to the Principal Investigator upon submission of a research proposal with ethical approval from the applicant's institution. This research is funded by Wellcome Trust and Gulu University.
Buckling Strength of Single Angle Struts Part 1: Angles Subject to Axial Compression
Authors: Chen Shaofan , Wang Xiantie 
Published: July 2013

Hot rolled angles are classified into two categories: equal-legged and unequal legged. They behave differently when subjected to axial compression owing to the difference in section symmetry: the equal-leg angle has one axis of symmetry while the unequal-leg one is asymmetric. This article aims at providing practical approaches for determining buckling strength of the two kinds of single angle struts subject to axial compression. For equal-leg angles, the first issue of concern is the failure mode. Although most researchers agree that equal-leg single angle buckles flexurally about the minor axis in the inelastic range as well as in the elastic range, some others got different conclusion. Through meticulous analysis based on tangent modulus concept, it is shown in this study that flexural-torsional buckling about the major axis is not the dominant failure mode. On the other hand, leg plate buckling may occur before the flexural buckling. So, a factor of effective yield strength is suggested for calculating the interactive local-flexural buckling, and the approach is verified with available test data. For unequal-leg angles, which always buckle in flexural-torsional mode, attention is focused on the simplification of the formula for calculating the critical load. Simplified formula of equivalent slenderness ratio is derived to transform the flexural-torsional buckling problem into a flexural one and is verified with available test data. The results arrived at in this study are recommended for routine design use.
Broadband Noise Predictions from Uninstalled Contra-Rotating Open Rotors
Authors: Blandeau Vincent P. , Joseph Phillip F. , Kingan Michael J. , Parry Anthony B. 
Published: July 2013

This paper presents broadband noise predictions from uninstalled contra-rotating open rotors (CRORs). CRORs are being increasingly investigated as an alternative to single propellers and turbofan engines for power plants on aircraft, since they yield a significant reduction in fuel burn for short-haul flights. However, there is the need to develop schemes by which CROR noise can be predicted and reduced. Using semi-analytical prediction schemes, the principal broadband noise source mechanisms (rotor trailing edge noise and rotor-rotor interaction noise) are investigated, leading to strategies for their mitigation. For the configuration considered, trailing-edge broadband noise is predicted to be dominant at low rotor loading conditions (typical of cruise and approach) whereas rotor-rotor interaction broadband noise is predicted to be dominant at high rotor loading conditions (typical of take-off). A systematic parameter study is also presented in which the dependence of CROR broadband noise on the rotor-rotor gap, rotor speeds and blade numbers are investigated at constant engine power, torque split and solidity.
Risk of Injury Associated with Skeletal Muscle Relaxant Use in Older Adults
Authors: Spence Michele M , Shin Patrick J , Lee Eric A , Gibbs Nancy E 
Published: July 2013

BACKGROUND The use of skeletal muscle relaxants (SMRs) among older adults is associated with sedation and confusion, which may lead to an increased risk of falls and injuries. SMRs continue to be used among older adults, although they are on the Beers list as drugs to avoid in the elderly. OBJECTIVE To investigate the relationship between SMR use and subsequent risk of injury. METHODS This was a retrospective case-control study of members aged 65 years or older enrolled in an integrated health care system. Cases were defined as patients with a documented injury resulting in either a hospitalization or an emergency department or urgent care visit from January 2009 through December 2010. Cases were matched to controls in a 1:4 ratio by age and sex. Patients had to be enrolled and alive on the date of an injury (index date). SMR exposure for all cases and controls was evaluated within 60 days prior to the index date. Conditional logistic regression adjusted for covariates was performed, with risk estimates presented as odds ratios with 95% confidence intervals. RESULTS From a base population of 322,806 older adults, we identified 27,974 cases of injury and 104,303 matched controls. Among the cases, 365 (1.30%) used an SMR; among the controls, 801 (0.77%) used an SMR in the 60 days prior to the index date. After adjustment for demographic and clinical covariates, risk of injury was significantly increased for patients using an SMR compared to no use (OR 1.32, 95% CI 1.16–1.50; p < 0.001). Carisoprodol was associated with an increased risk of injury (OR 1.73, 95% CI 1.04–2.88; p = 0.036), as were methocarbamol (OR 1.42, 95% CI 1.16–1.75; p = 0.001) and cyclobenzaprine (OR 1.22, 95% CI 1.02–1.45; p = 0.029). CONCLUSIONS Older adults using SMRs have an increased risk of injury. These findings provide evidence to support current recommendations to avoid the use of SMRs in elderly patients.
Standard Versus Prolonged Doripenem Infusion for Treatment of Gram-Negative Infections
Authors: Hsaiky Lama , Murray Kyle P , Kokoska Lianne , Desai Neha , Cha Raymond 
Published: July 2013

BACKGROUND Doripenem is the most recently introduced carbapenem, with a broad spectrum of antimicrobial activity. Preliminary data indicated that activity is optimized by maximizing the time that serum concentration remains above the minimum inhibitory concentration; however, limited clinical data are available to support this approach. OBJECTIVE To compare clinical outcomes before and after implementation of a hospital-wide initiative extending the duration of infusion for doripenem from 1 hour (standard) to 4 hours (prolonged). METHODS This retrospective, quasi-experimental study compared clinical outcomes associated with doripenem administered as a 1-hour infusion versus a 4-hour infusion for treatment of suspected or documented infections caused by gram-negative organisms. Outcomes were assessed for the entire cohort, as well as for the subpopulation of patients admitted to the intensive care unit. RESULTS Two hundred patients were included; 106 patients received doripenem via standard infusion and 94 patients via prolonged infusion. No significant differences were noted between the treatment groups in clinical success, length of stay, or duration of treatment when the entire cohort was evaluated. In the critically ill subgroup, pneumonia, standard-infusion doripenem, and concomitant bacteremia were independent predictors of clinical failure (adjusted odds ratio [95% CI] 7.8 [2.4–25.6], 5.5 [1.6–18.7], and 7.0 [1.6–31.3], respectively). Additionally, critically ill patients who received doripenem via standard infusion were significantly more likely to experience recurrence of infection or death within 90 days. No significant differences were noted in length of stay or duration of bacteremia. CONCLUSIONS The duration of infusion did not significantly impact outcomes when the entire cohort was compared; however, prolonged infusion of doripenem was associated with significantly improved clinical outcomes among critically ill patients. These findings support the use of prolonged infusion of doripenem for critically ill patients.