bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neuroglycome alterations of hippocampus and prefrontal cortex of juvenile rats chronically exposed to glyphosate-based herbicide
Authors: Solomon Joy , Gutierrez-Reyes Cristian D. , Chávez-Reyes Jesús , Onigbinde Sherifdeen , Marichal-Cancino Bruno A. , López-Lariz Carlos H. , Beck Mia , Mechref Yehia 
Published: August 2024

IntroductionGlyphosate-based herbicides (GBHs) have been shown to have significant neurotoxic effects, affecting both the structure and function of the brain, and potentially contributing to the development of neurodegenerative disorders. Despite the known importance of glycosylation in disease progression, the glycome profile of systems exposed to GBH has not been thoroughly investigated.MethodsIn this study, we conducted a comprehensive glycomic profiling using LC-MS/MS, on the hippocampus and prefrontal cortex (PFC) of juvenile rats exposed to GBH orally, aiming to identify glyco-signature aberrations after herbicide exposure.ResultsWe observed changes in the glycome profile, particularly in fucosylated, high mannose, and sialofucosylated N-glycans, which may be triggered by GBH exposure. Moreover, we found major significant differences in the N-glycan profiles between the GBH-exposed group and the control group when analyzing each gender independently, in contrast to the analysis that included both genders. Notably, gender differences in the behavioral test of object recognition showed a decreased performance in female animals exposed to GBH compared to controls (p &lt; 0.05), while normal behavior was recorded in GBH-exposed male rats (p &gt; 0.05).ConclusionThese findings suggest that glycans may play a role in the neurotoxic effect caused by GBH. The result suggests that gender variation may influence the response to GBH exposure, with potential implications for disease progression and specifically the neurotoxic effects of GBHs. Understanding these gender-specific responses could enhance knowledge of the mechanisms underlying GBH-induced toxicity and its impact on brain health. Overall, our study represents the first detailed analysis of N-glycome profiles in the hippocampus and PFC of rats chronically exposed to GBH. The observed alterations in the expression of N-glycan structures suggest a potential neurotoxic effect associated with chronic GBH exposure, highlighting the importance of further research in this area.
Clinicopathologic features and surgical treatment prognosis of esophageal carcinosarcoma
Authors: Shen Jiangfeng , Lu Kaijin , Liu Fuxing , Chen Xia , Chen Quan , Wu Bingbing , Wang Hailan , Ge Pengfei , Han Guang , Wang Fei , Zhang Peng , Yin Pei , Jia Weiguang , Zheng Yiming , Wang Pengcheng , Sun Fei 
Published: August 2024

BackgroundCarcinosarcoma is a rare esophageal tumor, accounting for approximately 0.27-2.8% of malignant esophageal tumors. This study aims to investigate the clinical pathological characteristics, surgical treatment outcomes, and analysis of prognostic factors in esophageal carcinosarcoma (ECS).MethodsClinical data from sixteen patients diagnosed with esophageal sarcomatoid carcinoma who underwent surgical interventions were retrospectively analyzed. Clinical and pathological features, treatment modalities, and postoperative outcomes were systematically examined.ResultsOut of the 1261 patients who underwent surgical treatment for esophageal cancer, 16 cases were pathologically confirmed as carcinosarcoma. Among them, two underwent neoadjuvant chemotherapy, six received postoperative chemotherapy. Carcinosarcomas predominantly occurred in the middle (43.75%) and lower (50%) segments of the esophagus. Among the 16 cases, 10 presented as polypoid, 4 as ulcerative, and 2 as medullary types. Microscopic examination revealed coexistence and transitional transitions between sarcomatous and carcinoma components. Pathological staging showed 5 cases in stage T1, 2 in stage T2, and 9 in stage T3, with lymph node metastasis observed in 8 cases (50%). TNM staging revealed 2 cases in stage I, 9 in stage II, and 5 in stage III. The overall 1, 3, and 5-year survival rates were 86.67%, 62.5%, and 57.14%, respectively. Univariate analysis indicated that pathological N staging influenced survival rates, while multivariate analysis demonstrated that pathological N staging was an independent prognostic factor.ConclusionsCarcinosarcoma is a rare esophageal tumor, accounting for approximately 0.27-2.8% of malignant esophageal tumors. Histologically, the biphasic pattern is a crucial diagnostic feature, although the carcinomatous component may not always be evident, especially in limited biopsies, leading to potential misclassification as pure sarcoma or squamous cell carcinoma. Despite its large volume and cellular atypia, carcinosarcoma carries a favorable prognosis. Complete surgical resection of the tumor and regional lymph node dissection is the preferred treatment approach for esophageal carcinosarcoma.
Outcomes of liver transplantation for hepatocelluler carcinoma from living donor versus deceased donor within University of Southern California San Francisco criteria: a report from Turkey
Authors: Batı Imam Bakır , Tüysüz Umut 
Published: August 2024

BackgroundHepatocellular cancer (HCC) is the most common primary liver cancer with increasing incidence. Liver transplantation (LT) has been accepted as main curative liver cancer treatment. The effectiveness of LDLT as opposed to Deceased Donor Liver Transplant (DDLT) for patients with HCC is still controversial. There is limited data comparing the long-term outcomes of patients undergoing LDLT or DDLT for HCCs that do not meet the Milan criteria.MethodsWe aimed to compare the perioperative and survival outcomes of LDLT with DDLT in HCC patients.Patients underwent LT between January 2012 and December 2020 were retrospectively analyzed. There were 137 patients who met the UCSF criteria. Of these, 75 patients received LDLT and 62 patients DDLT.The primary end points in the present study were oncologic outcomes such as the recurrence rate, disease-free survival (DFS) and overall survival (OS) of LDLT and DDLT in patients with HCC.ResultsPET-CT SUVmax value, the amount of erythrocyte solution (ES) as blood transfusion of red cells given and the tumor recurrence rate were significantly higher among the deceased patients recurrence, ES, PET-CT SUVmax value and tumor differentiation had significant effects on survival. In the multivariate reduced model, cox regression analysis showed significant effects of recurrence, ES, locoregional treatment response and PET-CT on survival.Albeit not significant, the one-year recurrence rate in the LDLT was similar to that in the DDLT, three- and five-year recurrence rates were higher in DDLT compared to LDLTConclusionThere is less chance of cold ischemia time and better-quality grafts with minimal fatty changes, lower recurrence rates and similar survival rates can be achieved in LDLT compared to DDLT
Transcriptome analysis of the aged SAMP8 mouse model of Alzheimer’s disease reveals novel molecular targets of formononetin protection
Authors: Liu Bo , Cui Di , Liu Jie , Shi Jing-Shan 
Published: August 2024

BackgroundSenescence-accelerated mouse prone 8 (SAMP8) and age-matched SAMR1 mice are used to study the pathogenesis and therapeutics of Alzheimer’s disease (AD); however, the molecular mechanisms are not completely understood.ObjectiveThis study aimed to examine the effects of the 5-month administration of formononetin in SAMP8 mice and used RNA-seq to explore the molecular targets.MethodsSAMP8 mice were orally administered formononetin (0, 8, and 16 mg/kg) from 4 months of age, and age-matched SAMR1 mice were used as controls. Behavioral tests were performed in 9-month-old mice, followed by histopathologic analysis. Total RNA from the hippocampus was isolated and subjected to RNA-seq, RT-qPCR, and bioinformatics analysis.ResultsThe 9-month-old SAMP8 mice exhibited cognition deficits, evidenced by novel object recognition, open-field test, elevated plus maze, and passive avoidance. Nissl bodies in the cortex and hippocampus were decreased. Formononetin treatments ameliorated behavioral deficits and improved morphological changes, which were evidenced by Nissl and H&amp;E staining. RNA-seq revealed distinct gene expression patterns between SAMP8 and SAMR1 mice. Differentially expressed genes in SAMP8 mice were attenuated or normalized by formononetin. Ingenuity pathway analysis (IPA) of canonical pathway and upstream regulators revealed increases in proinflammatory factors and immune dysfunction and decreases in NRF2 and SIRT-1 signaling pathways, leading to neuroinflammation. Formononetin treatment attenuated or reversed these molecular changes. The transcriptome of SAMP8 mice was correlated with transcriptomic profiles of other AD mouse models in the GEO database.ConclusionNeuroinflammation and decreased antioxidant and SIRT-1 signaling contributed to cognitive deficits in aged SAMP8 mice, which are potential therapeutic targets of formononetin in combination with other therapies.
A comparison of individual force decline profiles during a fatiguing eccentric trunk flexion and extension protocol: a pilot study
Authors: Paksoy Yasemin , Kpobi David , Henschke Jakob , Risch Lucie , Engel Tilman 
Published: August 2024

IntroductionMuscle fatigue, characterized by diminished force production and contraction sustainability, can impair muscle coordination and increase joint instability. Differing force profiles used in fatiguing tasks, such as prolonged eccentric trunk protocols, might provide insights into individualized strategies and resulting spinal stability. Thus, this study assessed individual differences in fatigue characteristics during an eccentric trunk flexion-extension protocol in a population of asymptomatic individuals.MethodsTwelve participants (2 f/10 m, 29 ± 4 years, 78.4 ± 16.9 kg, 1.76 ± 0.10 m) performed an eccentric trunk flexion and extension protocol on an isokinetic dynamometer (45° flexion to 10° extension; 60°/s), with final analysis on 8 participants for trunk flexion and 11 for trunk extension due to data exclusions. Participants engaged in a maximal all-out (AO) task for 2 min. Each participant's torque output (Nm) was assessed on a repetition-by-repetition basis, and smoothened by a moving average of 5 repetitions. Individual time profiles for reaching fatigue thresholds (10%, 15%, 20% and 30% reduction of initial torque output), and inter subject variability (by coefficient of variation, CV in %) were assessed throughout the AO task. Further, percentage torque reduction and variability were assessed at mid (1-minute) and end (2-minute) of task.ResultsOn average, for flexor and extensor muscles combined, participants reached a force reduction of 10% within 23.2 ± 19.1 s, of 15% within 44.9 ± 19.6 s, of 20% in 62.4 ± 26.3 s, and of 30% within 79.2 ± 21.8 s. The variability between individuals for the timepoint of reaching the defined torque thresholds was assessed by CV ranged between 23.4% and 103.8% for trunk flexor muscles, and between 28.4% and 56.5% for trunk extensor muscles.DiscussionA reduction of up to 20% was seen on average for all participants within 1-minute of eccentric trunk flexion and extension. Different inter-individual force output profiles were seen throughout the AO protocol, potentially related to physiological, skill-based, technical, adaptational, and/or motivational factors. The increase in fatigue resulted in a reduction in variability among individuals. A 2-minute protocol effectively induced pronounced fatigue, offering insights into individual force profiles and strategies.
Consideration of treatment goals and termination algorithm for adolescent atopic dermatitis using upadacitinib
Authors: Koga Takeshi , Okada Keisuke , Shimizu Takahiro , Morita Eiji , Itazawa Toshiko 
Published: August 2024

ObjectivesStandardized criteria for disease activity and end of treatment of systemic treatment of atopic dermatitis have not been established. The objective of this study is to explore the experience using upadacitinib retrospectively, to find clues to weaning from systemic treatment, and to determine the algorithm for setting treatment goals and terminating systemic treatment.MethodsFourteen patients treated with upadacitinib between 1 November 2021 and 31 December 2023 were enrolled in the study. Topical anti-inflammatory treatments were combined. Treatment outcomes were established according to the European Task Force of Atopic Dermatitis guidelines. To evaluate disease status and control, we adopted the Treat to target strategy. Changes in serum biomarkers (TARC and IgE) were also observed.ResultsAll patients achieved EASI 50 after 52 weeks. At 76 weeks, 80% and 30% of patients achieved EASI 75 and EASI 90, respectively. Four patients completed upadacitinib, five patients discontinued treatment, and five patients remained on treatment. Two patients achieved complete remission without topical anti-inflammatory treatment. Two patients discontinued due to adverse events. Patients with better treatment adherence, mainly topical treatment, tended to be able to withdraw from UPA. IgE increased from baseline in 11 patients (78.6%) and TARC increased in 14 patients (100%). These biomarkers decreased from the peak 24–48 weeks after initiating treatment, after the improvement of eczema.ConclusionCombining systemic and topical treatments effectively induces remission in AD patients. Transitioning off systemic treatment begins by achieving remission maintained solely with topical therapy, emphasizing the importance of adherence.
INFLUENCE OF GROWTH-REGULATING SUBSTANCES ON THE PROCESS OF ROOT FORMATION OF GOOSEBERRIES AT THE “ROOTING IN VITRO” STAGE
Authors: Кузнецова Ирина Борисовна , Макаров Сергей Сергеевич 
Published: August 2024

В статье приведены результаты исследований по изучению влияния различных концентраций ИМК и добавки Экогеля на корнеобразовательный процесс растений крыжовника на этапе «укоренение in vitro». Закладка плантаций высококачественным оздоровленным посадочным материалом, полученным методом клонального микроразмножения, является необходимым условием получения высоких и регулярных урожаев крыжовника. Использование регуляторов роста при клональном размножении, позволяет управлять процессами регенерации растений. Содержание в питательной среде Мурасиге-Скуга ауксина ИМК способствует значительному увеличению количества корней у растений-регенерантов крыжовника (в среднем - 3,1 шт.), в варианте с ИМК 0,5 мг/л - 5,4 шт., с ИМК 1,0 мг/л - 6,3 шт. Добавление в питательную среду Экогеля в концентрации 0,5 мг/л способствовало увеличению количества корней в среднем от 4,8 до 5,1 см. Выявлено, что при повышении концентрации ауксина ИМК от 0,5 до 1,0 мг/л в питательной среде MS количество корней и суммарная длина растений-регенерантов крыжовника увеличивались, а средняя длина немного уменьшалась. Наличие в питательной среде Экогеля в концентрации 0,5 мг/л способствует значительному увеличению суммарной длины корней (в среднем - 13,1 см), без Экогеля - 10,8 см. При добавлении в питательную среду Экогель в концентрации 0,5 мг/л оказал благоприятное влияние на процесс корнеобразования.
The results of studies investigating the effect of various concentrations of IMС and Ecogel additives on the root formation process of gooseberry plants at the “in vitro rooting” stage. Creation of plantations with high-quality healthy planting material obtained by the method of clonal micropropagation is a prerequisite for obtaining high and regular yields of gooseberry. The use of growth regulators during clonal propagation allows controlling the processes of plant regeneration. The content of auxin IMC in the Murashige-Skoog nutrient medium of promotes a significant increase in the number of roots in gooseberry regenerant plants (on average 3.1 pcs.), in the variant with IMC 0.5 mg/l - 5,4 pcs., with IMC 1,0 mg/l - 6,3 pcs. The addition of Ecogel at a concentration of 0,5 mg/l to the nutrient medium contributed to an increase in the number of roots on average from 4,8 to 5,1 cm. The number of roots and the total length of gooseberry regenerated plants increased and the average length decreased slightly with an increase in the concentration of auxin IMC from 0,5 to 1,0 mg/l in the MS nutrient medium. The presence of Ecogel at a concentration of 0,5 mg/l in the nutrient medium contributes to a significant increase in the total length of the roots (13,1 cm on average), without Ecogel - 10,8 cm. The addition of Ecogel to the nutrient medium at a concentration of 0,5 mg/l had a beneficial effect on the root formation process.
INFLUENCE OF PROTEIN MEDICATION FROM MARROW CELLS ON LABORATORY ANIMALS IMMUNITY CELL-MEDIATED INDICES
Authors: Федоренко Татьяна Валериевна , Мандро Николай Михайлович 
Published: August 2024

В настоящее время фармацевтической промышленностью на рынок выпускается большое количество иммуностимуляторов и иммуномодуляторов с различным механизмом их действия на отдельные звенья иммунитета. При этом разработка новых препаратов остается актуальной, так как иммунодефициты различной этиологии снижают эффективность лечения отдельных болезней и специфической профилактики, в связи с чем ветеринарному врачу всё чаще приходится применять иммуностимуляторы с целью повышения иммунного статуса организма животных. В статье представлены результаты исследования влияния белковых препаратов, изготовленных из клеток костного мозга сибирской косули (ПКМК), изюбря (ПКМИз), лося (ПКМЛ) на иммунобиологические показатели крови белых мышей (n=72). Препараты представляют собой водный раствор, содержащий в ПКМК 14,91±0,31 г/л, в ПКМИз - 15,0±0,38 г/л, ПКМЛ - 13,7±21 г/л общего белка. Препараты вводили подкожно в дозе 0,25 мл на одну голову, однократно . Проведенные исследования показали увеличение иммунокомпетентных клеток в крови. Так, количество лейкоцитов увеличилось в среднем на 30-33% за счет увеличения содержания лимфоцитов, моноцитов. На фоне применения белковых препаратов увеличились показатели фагоцитарной активности нейтрофилов, тем самым повышая иммунологический статус лабораторных животных. Все применяемые нами белковые препараты практически в равной степени оказывают положительное влияние на показатели клеточного иммунитета лабораторных животных.
Nowadays a lot of immunostimulants and immunomodulators with different effect on individual components of immune system are brought to the market by pharmaceutical industry. The development of new medication continues to be relevant as immunodeficiency of different etiology decreases effectiveness of individual diseases treatment and specific prophylaxis; with reference of this a veterinarian has to use immunostimulants in order to increase immune status of animals’ organism. In this article the research results of influence of protein medication made of Siberian roe deer (PKMK), red deer (PKMIz), elk (PKML) marrow cells on immunobiological blood indices of white mice (n = 72) are presented. The preparations are an aqueous solution containing 14.91 ± 0.31 g / l in PKMK, 15.0 ± 0.38 g / l in PKMIz, and 13.7 ± 21 g / l of total protein in PKML. The drugs were injected subcutaneously at a dose of 0.25 ml per head, once. The conducted researches showed the increase of immuno-competent cells in blood as leucocytes quantity rose on average by 30-33% at the expense of content of lymphocytes and monocytes. Due to protein medication usage the indices of neutrophil phagocytal activity have become higher, as a result the laboratory animals’ immunological status increased. All used protein medications almost equally give a positive effect on laboratory animals’ cell immunity.
BIOLOGIZATION AS AN ALTERNATIVE TO SOIL REPRODUCTION IN THE REPUBLIC OF BURYATIA
Authors: Батудаев Антон Прокопьевич , Батуева Марина Бадмацыреновна , Цыбиков Бэликто Батоевич , Алтаева Ольга Алексеевна , Бутуханов Анатолий Богомолович 
Published: August 2024

Главные требования органического земледелия - получение высоких и стабильных урожаев сельскохозяйственных культур без применения минеральных удобрений промышленного производства и различных средств защиты растений от вредных объектов. В статье представлены результаты полевых опытов, проведенных в трех основных земледельческих зонах Республики Бурятия (сухостепной, степной и лесостепной). Рассмотрено влияние полевых севооборотов на каштановой, черноземной и серой лесной почве в условиях опытного поля кафедры общего земледелия БГСХА им. В.Р. Филиппова. Приведены выводы и заключения по полевым опытам по изучению полевых севооборотов с включением донника на черноземной почве, показана удобрительная эффективность соломы и влияние на плодородие каштановой почвы её диспергирования. Установлена высокая урожайность зеленой массы этой культуры и позитивное влияние на плодородие малогумусного чернозема. Рассмотрены различные сельскохозяйственные культуры (горох, вика, суданская трава, овес, рапс яровой и редька масличная) в сидеральных парах. Для достижения наибольшего удобрительного эффекта на малоплодородных серых лесных почвах в сидеральных парах рекомендуется использовать бобовые (горох и вика) и капустные культуры - рапс и редьку масличную
The main requirements of organic farming are to obtain high and stable crop yields without the usage of industrially produced mineral fertilizers and various plant protection agents against harmful objects. The article presents the results of field experiments conducted in the three major cropping zones of the Republic of Buryatia (dry-steppe, steppe and forest-steppe). The effect of field crop rotations on chestnut, chernozem and gray forest soils in the conditions of the experimental field of the General Farming Department of the BSAA named after V.R. Filippov is considered. The article provides the outputs and conclusions on the field experiments on the study of field crop rotations with the inclusion of melilot on the chernozem soil. It also presents the fertilizing efficiency of straw and the influence of its dispersion on the fertility of the chestnut soil. The high yield of green mass of this crop and positive influence on fertility of low-humus chernozem have been found. Different agricultural crops (such as peas, vetch, Sudan grass, oats, spring rapeseed and oil radish) on the green-manure fallows have been considered. To achieve the greatest fertilizing effect on low fertility gray forest soils on green-manure fallows it is recommended to use legumes (peas and vetch) and cabbage crops - rapeseed and oil radish.
PENERAPAN MODEL PEMBELAJARAN SNOWBALL THROWING UNTUK MENINGKATKAN HASIL BELAJAR SISWA PADA PELAJARAN PENGETAHUAN BAHAN MAKANAN KELAS X JASA BOGA
Authors: Teang Maria Viana , Suryanto I Wayan , Astuti Ni Made Erpia Ordani 
Published: August 2024

Penelitian ini merupakan jenis penelitian tindakan kelas yang dilaksanakan pada semester genap tahun pelajaran 2016/2017 dengan metode pengumpulan data, observasi, tes dan dokumentasi. Tujuan penelitian ini adalah untuk mengetahui peningkatan hasil belajar siswa kelas X jasa boga pada pelajaran pengetahuan bahan makanan di SMK Wira Harapan dengan model pembelajaran snowball throwing. Analisis data menggunakan analisis deskriptif kuantitatif. Hasil penelitian menunjukkan: 1) terjadi peningkatkan hasil belajar siswa kelas X jasa boga 3 di SMK Wira Harapan setelah diterapkannya model pembelajaran snowball throwing pada mata pelajaran pengetahuan bahan makanan tahun pelajaran 2016/2017. Hal ini dapat dilihat dari hasil belajar siswa pada siklus I memperoleh rata-rata 69,59% berada pada kategori “cukup” sedangkan pada siklus II rata-rata hasil belajar siswa 81,29% berada pada kategori “baik”. Hal ini menunjukkan telah terjadi peningkatan hasil belajar siswa dari siklus I ke siklus II sebesar 11,7%; 2) terjadi peningkatkan ketuntasan klasikal siswa kelas X jasa boga 3 di SMK Wira Harapan setelah diterapkan model pembelajaran snowball throwing pada mata pelajaran pengetahuan bahan makanan tahun pelajaran 2016/2017. Hal ini dapat dilihat dari ketuntasan klasikal siswa pada siklus I memperoleh rata-rata 40,54% berada pada kategori “kurang” sedangkan pada siklus II rata-rata ketuntasan klasikal siswa 100% berada pada kategori “baik”. Hal ini menunjukkan telah terjadi peningkatan ketuntasan klasikal siswa dari siklus I ke siklus II, peningkatan rata-rata sebesar 59,46%.