bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rumen microbes associated potential to establish climate resilience in ruminants
Authors: Silpa M.V. , Kalaignazhal G. , Binuni Rebez E. , Devaraj C. , Tüfekci H. , Mylostyvyi Roman , Thanislass J. , Sahoo A. , Dunshea F.R. , Sejian V. 
Published: February 2025

Abstract
Ruminants are stated to play a pivotal role of converting feed resources into nutritious outputs suitable for human consumption, which primarily is attributed to the diversified rumen microbiota they harbour. The rumen microbial niche is however not explored to its complete potential due to several limitations, the crucial one being the inability to culture more than 80% of the rumen microbes. Advances in next generation sequencing approaches have made promising inroads to bridge this gap. The adoption of techniques like 16S rRNA sequencing and whole genome shotgun metgenomics has now made it possible to assess the structural and functional dynamics of rumen microbiota. Nevertheless, there is a dearth of information pertaining to the impact of climate change on rumen microbe. This review was therefore an attempt to highlight the potential role of rumen microbiome during heat stress and also to emphasize on the significance of adopting rumen metagenomics to link climate change impact with altered productive potential in ruminants. Changes in ruminal microflora like, alterations in Firmicutes-Bacteroidetes ratio (highly correlated with milk fat yield), altered abundance of Fibrobacters group (potentially heat generating microbes), Actinobacteria group (acetate producing microbes), Fusobacteria, Cyanobacteria and so on were observed in ruminants during heat stress. Furthermore, it was also possible to link these alterations with several functional and metabolic pathways like, energy production and conversion, defense mechanism, lipid transport and metabolism, coenzyme transport and metabolism, cell cycle control, cell division and few more. Employing rumen metagenomics could also aid in understanding the differences in heat stress adaptive strategies between breeds. Therefore, intensifying the application of this methodology with heat stress in ruminants and extending it to assess the effectiveness of nutritional amelioration strategies could embark a new journey towards heat stress mitigation in ruminants.
Collection, preservation, and assessment of canine epididymal semen – current knowledge and future challenges
Authors: Pulkowska-Bluj Oksana , Trzcińska Monika 
Published: February 2025

Abstract
Epididymal spermatozoa have great potential as an alternative source of canine germ cells for the preservation of the unique features of both endangered canid species and purebred dogs. Epididymal semen collected from exceptional individuals is usually cryopreserved and constitutes the final reserve of desired biological material. Cryopreservation of semen recovered from the epididymis poses difficulties related to obtaining samples at sufficient concentrations, in addition to its lower cryotolerance than ejaculated semen. Meeting the challenges associated with the conservation of epididymal sperm necessitates delving deeper into the available methods for its acquisition and preservation, in addition to applying appropriate evaluation methods. The quality of cryopreserved epididymal sperm can be influenced by a number of factors, from the collection technique applied to the insemination process utilized. Furthermore, our review provides a comparison of the effectiveness of selected extenders and their supplements and highlights aspects that require improvements or further investigation. The analyzed literature data show that squeezing and retrograde flushing technique of obtaining semen from the epididymis minimizes tissue and erythrocytes contamination. In order to ensure the best quality of epididymal semen after thawing, storage in 4-5°C should be avoided for longer than: 24 h in 0.9% saline or 5 days in chilling extenders. We concluded that the egg yolk based extenders and Bovimix® are the most efficient methods of cryopreservation of canine epididymal sperm, however the use of supplementation such as antioxidants requires further investigation.
Dietary supplementation of Lactobacillus sakei subsp. sakei on the growth performance, immune responses, and antioxidant defense of rainbow trout (Oncorhynchus mykiss) exposed to the herbicide butachlor
Authors: Yue Feng , Zhou Juanjuan , Ramezani Sudabe , Ghafarifarsani Hamed 
Published: February 2025

Abstract
The aim of this work was to determine the benefits of dietary supplementation with the probiotic Lactobacillus sakei subsp. sakei (LS) on the growth, intestinal microbiota, digestive enzymes, immunological responses, humoral antioxidant markers and liver enzymes of rainbow trout (Oncorhynchus mykiss) exposed to the herbicide butachlor (BU). 6 experimental groups including C1: non-supplemented and non-exposed fish, LS1: 1×106 (cfu/g) L. Sakei, LS2: 1×108 (cfu/g) L. Sakei, BU: 12.5% LC50 butachlor, LS1 + BU: 1×106 (cfu/g) L. Sakei + 12.5% LC50 butachlor, LS2 + BU: 1×108 (cfu/g) L. Sakei + 12.5% LC50 butachlor were designed. After the 30-day experimental period, a significant increase was observed in the survival rate (SR), final weight (FW), and weight gain (WG) of the fish fed with LS (P<0.05). The total intestine bacteria (TBC) elevated in the groups supplemented with probiotic, while a significant decrease was observed in the fish exposed to butachlor (P<0.05). Fish fed with probiotic indicated a higher value of protease activity, while significant decrease observed in protease activity in the butachlor group (P<0.05). The fish of probiotic group showed a significant increase in immunity parameters components including lysozyme and total Ig. Furthermore, an improvement was observed in hormonal antioxidant markers [superoxide dismutase (SOD) and catalase (CAT)] (and liver enzymes in the probiotic groups (P<0.05). Overall, results suggested a protective role for LS against the harmful effects of butachlor through restoring gut health, the digestive enzyme activities, immune and antioxidant responses in the rainbow trout.
Impact of herbal blends supplementation on the mineral profile of blood plasma and milk of dairy cows
Authors: Kowalczuk-Vasilev Edyta , Fabjanowska Julia , Klebaniuk Renata , Kiczorowska Bożena , Samolińska Wioletta , Witkowska-Piłaszewicz Olga 
Published: February 2025

Abstract
The present study aimed to evaluate the effect of supplementation of cow´s diet with four different herbal blends on the mineral profile in the plasma and milk of cows in the first stage of lactation. The experiment involved 50 cows divided into five groups. Four herbal blends were used as an experimental factor. A full dose of 100 g of the herbal supplements per 100 kg b.w. per day was included in the cow´s diet to the cows from one week before the planned calving to the end of the third month of lactation. The feed intake by the dairy cows was assessed in three 3-day cycles in lactation weeks 6, 7, and 8. Concurrently, blood and milk were collected from these cows. The content of selected minerals was determined in the feed rations, herbal blends, milk, and plasma. The study showed that herbs, besides being a source of bioactive compounds, should be considered a beneficial source of minerals, especially microelements like copper, zinc and iron, and might affect the mineral homeostasis in the organism. The introduction of different herbal blends affected not only the mineral profile of blood but most notably the milk mineral profile.
Gut dysbiosis: causes and prevention strategies
Authors: Attia Youssef A. , Helal Ibrahim E. , Nagadi Sameer A. , Khafaga Asmaa F. , Taha Ayman E. , Asiry Khalid A. , Abdulsalam Nisreen M. , Zabermawi Nidal M. , Shafi Manal E. , Zakaria Hana A. , Ebeid Tarek A. , Al-Homidan Ibrahim H. , Bovera Fulvia , Tufarelli Vincenzo 
Published: February 2025

Abstract
Gut dysbiosis in poultry presents a major challenge, requiring a comprehensive approach to sustain optimal gut health and ensure animal welfare. This review emphasizes the importance of integrated management practices and explores alternatives to antibiotics, including probiotics, prebiotics, synbiotics, postbiotics, phytogenic compounds, medicinal plants, and organic acids. Additionally, it advocates tailored nutritional strategies specific to broiler strains and highlights the potential of advanced biotechnologies for poultry nutrition and health management. Maintaining effective gut health is essential not only for animal welfare but also for the economic sustainability of poultry farming. Given the implications of antibiotic use in poultry for human health, the “One Health” approach is critical for recognizing the interconnection between animal, human, and environmental health. Research should prioritize understanding host-microbiome interactions, developing innovative therapies, and enhancing diagnostic tools for early detection and intervention in gut dysbiosis. Addressing this complex issue requires collaboration among researchers, veterinarians, feed manufacturers, and poultry producers to create sustainable solutions that improve animal health and production efficiency, while reducing reliance on antibiotics. Implementing a holistic strategy that encompasses various aspects of poultry management. The industry can mitigate the adverse effects of gut dysbiosis and promote the overall health and welfare of broiler chickens.
Acorn Flour as A Functional Ingredient: Impacts on Health and Baking Properties in Wheat - Based Products
Authors: Sikora Fryderyk , Ochmian Ireneusz , Sobolewska Magdalena 
Published: February 2025

Abstract
This study examines the potential of acorn flour derived from three different oak species - pedunculate oak, sessile oak, and red oak - as a partial substitute for wheat flour in bread making, aiming to leverage the underutilized acorn as a sustainable and health-promoting food resource. Various proportions (5%, 10%, and 15%) of acorn flour were incorporated into wheat flour, and the effects on bread quality, nutritional enhancement, and consumer acceptability were systematically evaluated. Results indicate that acorn flour significantly enriches the bread with essential minerals like potassium and calcium while also introducing beneficial phenolic compounds, enhancing antioxidant properties without compromising the sensory attributes of the bread. The study confirms that up to 15% substitution of wheat flour with acorn flour from red oak in particular does not detrimentally affect the bread's structural integrity and is well-received by consumers, suggesting its feasibility for commercial baking. The conclusions underscore acorn flour's potential as a functional ingredient that not only enhances bread's nutritional profile but also contributes to food sustainability.
LSTM-Based Discrimination of Date Fruit (Phoenix dactylifera L.) Based on Selected Convolutional Neural Network Features
Authors: Noutfia Younés , Sabanci Kadir , Aslan Muhammet Fatih , Ropelewska Ewa 
Published: February 2025

Abstract
Date palm (Phoenix dactylifera L.) is one of the most valuable domesticated fruit trees characterized with thousands of varieties that grow in different arid regions. Because of high diversification, discrimination between date varieties at different post-harvest handling and production stages is necessary. In this study, five different CNN (Convolutional Neural Network) models, namely ResNet18, ResNet50, MobileNet, GoogleNet and DenseNet, are used as fine-tuning tools for the classification of five Moroccan date fruit varieties: ‘Mejhoul’, ‘Boufeggous’, ‘Assiane’, ‘Aziza’ and ‘Bousthammi’. The features of MobileNet, the most successful of these CNN models, were analyzed with an RNN (Recurrent Neural Network)-based LSTM (Long short-term memory) architecture. In addition, feature selection is performed for MobileNet features to achieve a more successful classification with fewer features. As a result of LSTM-based classification of both original MobileNet features and selected features, higher classification accuracy was achieved in comparison with other CNN models. Moreover, LSTM with selected features provided the most successful discrimination ability. The accuracies obtained as a result of the classification of original MobileNet features and selected features with LSTM were 99.63% and 99.70% respectively. Overall, the results indicated that the LSTM-based architecture with fewer features improves the success of existing CNN models for date fruits.
Impact of Intensive and Semi-Intensive Farming on Histological Features and Culinary Properties of Common Carp (CYPRINUS CARPIO L.) Fillets
Authors: Eljasik Piotr , Sobczak Małgorzata , Lisiecki Sławomir , Sadowski Jacek , Panicz Remigiusz 
Published: February 2025

Abstract
Eco-intensification of freshwater aquaculture production is considered one of the solutions to achieve global food security. However, intensification of the production may come with product quality trade-offs, which are unlikely to be accepted by modern consumers. The aim of this study was to evaluate impact of intensive cage farming and typical semi-intensive pond culture on culinary and histological characteristics of common carp (Cyprinus carpio L.) fillets. In intensive cage culture common carp were fed formulated feed for 60 days, while in semi-intensive pond farming fish utilised natural food and supplementary grains (triticale) for 90 days to achieve similar weight. Next, fish were sacrificed, filleted and quality of fillets, i.e. pH, colour, histology of muscle tissue, texture and sensory properties were assessed. The results showed that intensive cage culture, because of constant water flow, contributed to muscle development through hypertrophy mechanism and resulted with higher number of large muscle fibers (over 60 μm in diameter) compared to semi-intensive pond farming. The differences in histology of muscle tissue were correlated with sensory characteristics of fillets, but not with texture instrumental analysis. Appearance (colour) and taste of the fillets of common carp from semi-intensive pond farming was more attractive from consumers’ perspective, especially that no negative features, such as off-flavour were noted compared to intensive cage culture.
The Impact of the Ozonation Process on the Content Of Bioactive Compounds in Zucchini Fruits and the Concentration of Selected Gases During Their Storage
Authors: Zardzewiały Miłosz , Basara Oskar , Belcar Justyna , Balawejder Maciej , Gorzelany Józef 
Published: February 2025

Abstract
The article presents the impact of ozone gas on the mechanical, chemical and microbiological parameters of zucchini fruit. Moreover, the influence of gaseous ozone on the concentration of gases in storage chambers was determined. Immediately after harvesting, zucchini fruits were treated with ozone at the following doses: 10 ppm -15 and 30 min; 50 ppm -15 and 30 min) in order to modify selected bioactive compounds Then the ozonation process was repeated every 48 hours. Zucchini fruits were stored in a climatic chamber at 7°C and 90% humidity. Laboratory analyses were performed on the 1st and 10th day of storage. As a result of the use of the tested variable factor, no significant changes in the mechanical properties were noted. However, a significant effect of selected doses of ozone gas, i.e. 10 ppm 30 minutes and 50 ppm 15 minutes on the 10th day of storage, on the increase in vitamin C concentration compared to the control was demonstrated. In the case of the total polyphenol content, there was also a significant effect of the ozone dose of 50 ppm applied for 15 and 30 minutes on the 1st day of storage on the increase in the value of this parameter compared to the control. The antioxidant potential in the ABTS test increased significantly on the 1st day of storage after applying an ozone dose of 50 ppm for 15 and 30 minutes. In turn, in the DPPH test, only for an ozone dose of 10 ppm for 15 and 30 minutes on the 10th day of testing, a significant increase in the tested parameter was recorded. The positive effects of ozone use were observed during the storage of zucchini fruits in terms of reducing the microbiological load. . The 50 ppm dose applied for 15 and 30 minutes had the most beneficial effect on reducing the number of aerobic mesophilic bacteria and the number of yeasts and molds. Ozone gas is an important factor inhibiting the production of ethylene by zucchini fruit during storage, thus extending the storage life of the tested raw material.
Cement Kiln Dust with Fine Volcanic Ash as Substitute Fillers for Hot Mix Asphalt
Authors: Alsharafi Mohammed A.A. , Almaswari Abdullah A. , Algaboby Ziyad M. 
Published: February 2025

Abstract
Waste and by-product materials are often used as substitutes for raw materials in asphalt mixtures to lessen building costs, minimize environmental problems, and protect natural resources. This study investigates the feasibility of employing two kinds of ashes, fine volcanic ash (FVA) and cement kiln dust (CKD), which are abundantly available in various regions of Yemen, as substitute hot mix asphalt filler (HMA) for conventional fillers. A traditional filler, basalt filler (BF), was partially and totally replaced at different percentages. FVA replaced 15%, 30%, 45%, 60%, and 100% by weight of the filler. CKD replaced the traditional filler with 25%, 50%, 75%, and 100% by weight of fillers as a partial replacement. The total replacement of traditional fillers includes 20% CKD + 80% FVA, 40% CKD + 60% FVA, 60% CKD + 40% FVA, and 80% CKD + 20% FVA. The evaluation of the planned HMA was done using conventional mechanical and volumetric characteristics. Marshall stability, direct compression (DC) tests, and indirect tensile strength (ITS) were employed to evaluate the influence of FVA with CKD fillers on the asphalt mixture’s mechanical characteristics. The outcomes of the experiment demonstrated that 60% CKD + 40% FVA was used instead of the traditional mineral filler. A novel HMA was introduced, demonstrating enhanced stability, increased bulk density, decreased voids filled with bitumen (VFB), and lower flow compared to the mixture used as a control. Results showed that FVA and CKD may respectively substitute 30% and 75% of the BF. The ITS and the DC exhibited an increase. Therefore, incorporating CKD and FVA as fillers in is a sustainable solution that enhances performance, durability, and environmental benefits in asphalt construction.
Performance Evaluation of Asphalt Concrete Wearing Containing Plastic Bags Using the Dry Method Under Various Thermal Cycles
Authors: Bousmaha Youssra , Merbouh M’hammed , Koting Suhana , Babalghaith Ali Mohammed 
Published: February 2025

Abstract
Premature technical issues in roads and highways worldwide often stem from inadequate asphalt concrete composition or intense traffic loads under varying climatic conditions. This study evaluated the performance of both original and low-density polyethylene (LDPE)-modified asphalt concrete under various thermal cycling conditions. Three thermal cycling ranges [-20°C to +20°C, +20°C to +40°C, and +40°C to +60°C] were applied to assess the mixtures’ mechanical performance. The LDPE modification involved replacing 5% of the bitumen weight with dry plastic bags. Performance was assessed using Marshall stability, dynamic creep, wheel tracking, and resilient modulus tests. Results indicated that LDPE modification significantly improved Marshall stability, reduced permanent deformation, and increased the stiffness modulus of the asphalt concrete. Notably, freezing-thawing cycles [-20°C to +20°C] caused more damage to the asphalt concrete compared to heating-cooling cycles. Moreover, the LDPE-modified mixture demonstrated enhanced performance across all thermal cycling ranges, suggesting its potential to improve road durability under diverse climatic conditions.
Parameters and Characteristics for Choosing the Type of Asphalt Mixture for the Road Wearing Course
Authors: Carmen Răcănel , Claudia Petcu 
Published: February 2025

Abstract
The pavement is the reinforced part of the road or street on which vehicular traffic travels. Pavement for road consist of different road layers, the choice of their composition (order of layers) and characteristics (thickness, quality) depending mainly on the traffic as well as other factors: climatic conditions, nature of the soil in the foundation, local materials, gradients, working technologies.
The choice of wearing course is one of the most important stages in the design of a road structure, both for a road being rehabilitated and for a new road. The performance of the asphalt mixture, which is used as wearing course, is very important.
In the present study the following types of tests were considered in order to highlight the characteristics of the types of asphalt mixtures and types of bitumen used: resistance to permanent deformation (wheel tracking) in accordance with SR EN 12697-22, resistance to fatigue is determined according to SR EN 12697-24, Annex A (Two – point bending test on trapezoidal shaped specimens) and Annex E (Indirect tensile test on cylindrical shaped specimens), triaxial cyclic compression test according to SR EN 12697-25 and indirect tensile test on cylindrical shaped specimens (IT – CY) was carried out in accordance with SR EN 12697-26, Annex C.
Attachment geometry and clinical predictability in aligner treatment: A systematic review
Authors: Ben Mohimd Hajar , Oualalou Youssef , Zaoui Fatima , Houb-Dine Afaf , Benyahia Hicham 
Published: February 2025

Objectives
The aim of this systematic review was to assess attachment efficiency in the improvement of movement predictability in aligner treatment and to determine the impact of their type (conventional or optimized) on movement accuracy.

Material and Methods
Three electronic databases (PubMed, ScienceDirect, and Cochrane Library) were screened from initiation to December 2023 for studies assessing attachment efficacy using this search equation (Aligners OR Invisalign OR [clear aligners] OR [transparent aligners]) AND (attachments) AND (efficacy OR efficiency OR effectiveness) AND (orthodontics). Quality assessment of the included studies was performed using the JBI tool to assess cohort and case-control studies and Risk of Bias 2 (RoB 2) in randomized trials.

Results
A total of 970 publications were found as search results in the screened databases. Nineteen were included in this systematic review based on pre-determined inclusion criteria. The studies were published between 2009 and 2023. The analysis of the results was realized by examining all types of movements. RoB assessment in this systematic review was moderate in the majority of the studies.

Conclusion
The current systematic review showed that the use of attachments could improve movement accuracy. However, more interventional studies are necessary with control groups to support and confirm the results of the included studies.