bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved separation of fluorogenic derivatized intact proteins with high resolution and efficiency using a reversed‐phase liquid chromatographic system
Authors: Ichibangase Tomoko , Nakata Katsunori , Imai Kazuhiro 
Published: May 2014

ABSTRACTAlthough the efficient separation of intact protein mixtures is extremely difficult, reversed‐phase chromatography is an important technique for performing quantitative, accurate and reproducible proteomics analyses. Here, we show that, despite the operating constraints of conventional high‐performance liquid chromatography, such as column temperature, operating pressure and separation time, comprehensive separation of fluorogenic derivatized intact proteins could be achieved with high resolution and separation efficiency. First, amylin was chosen as a model peptide and used to estimate the separation efficiency with respect to column temperature and flow rate, as indicated by peak capacity. Then, an extract of human primary hepatocytes was used to model complex component mixtures and the separation conditions were optimized. The effects of mobile‐phase pH, the separation time and the column length were also investigated. Consequently, more than 890 peaks could be separated efficiently in the extract, which is 1.5‐fold greater than when using conventional conditions. Finally, it was demonstrated that both longer separation time and column length contributed greatly to the effective separation of the protein mixture. These results are expected to provide insights into the separation of intact proteins. Copyright © 2014 John Wiley & Sons, Ltd.
Enantioseparation of meptazinol and its three intermediate enantiomers by capillary electrophoresis using a new cationicβ‐cyclodextrin derivative in single and dual cyclodextrin systems
Authors: Yu Jia , Zhao Yunfeng , Song Jiaxin , Guo Xingjie 
Published: May 2014

ABSTRACTA new cationicβ‐cyclodextrin derivative, mono‐6‐deoxy‐6‐piperdine‐β‐cyclodextrin (PIP‐β‐CD), was synthesized and applied as a chiral selector for the enantioseparation of meptazinol and its three intermediate enantiomers (intermediates II–IV) in capillary electrophoresis. When PIP‐β‐CD was employed as the single CD system, intermediate II was baseline enantioseparated while the results for the other analytes were less satisfactory. In order to enhance the selectivity and resolution of meptazinol intermediate III and intermediate IV, dual CD systems of PIP‐β‐CD in combination with three different neutral CDs were synthesized and tested:β‐cyclodextrin (β‐CD), trimethyl‐β‐cyclodextrin (TM‐β‐CD) and hydroxypropyl‐β‐cyclodextrin (HP‐β‐CD). After investigating the effect on the enantioseparation efficiency of the nature and the concentration of both CDs, the dual CD system consisting of PIP‐β‐CD and HP‐β‐CD was shown to be the most efficient for the simultaneous enantioseparation of meptazinol intermediates III and IV. Copyright © 2014 John Wiley & Sons, Ltd.
Dried saliva spot as a sampling technique for saliva samples
Authors: Abdel‐Rehim Abbi , Abdel‐Rehim Mohamed 
Published: May 2014

ABSTRACTFor the first time, dried saliva spot (DSS) was used as a sampling technique for saliva samples. In the DSS technique 50 μL of saliva was collected on filter paper and the saliva was then extracted with an organic solvent. The local anesthetic lidocaine was used as a model compound, which was determined in the DSS using liquid chromatography and mass spectrometry. The results obtained for the determination of lidocaine in saliva using DSS were compared with those from a previous study using a microextraction by packed sorbent syringe as the sampling method for saliva. This study shows that DSS can be used for the analysis of saliva samples. The method is promising and very easy in terms of sampling and extraction procedures. The results from this study are in good agreement with those from our previous work on the determination of lidocaine in saliva. DSS can open a new dimension in the saliva handling process in terms of sampling, storing and transport. Copyright © 2014 John Wiley & Sons, Ltd.
Kidney tissue targeted metabolic profiling of glucocorticoid‐induced osteoporosis and the proposed therapeutic effects of Rhizoma Drynariae studied using UHPLC/MS/MS
Authors: Huang Yue , Liu Xinyu , Zhao Longshan , Li Famei , Xiong Zhili 
Published: May 2014

ABSTRACTTraditional Chinese medicine and modern science have indicated that there is a close relationship between bone and kidney. In light of this, this project was designed to study the metabolic profiling by UHPLC/MS/MS of glucocorticoid‐induced osteoporosis in kidney tissue and the possible therapeutic effects of Rhizoma Drynariae (RD), a classic traditional Chinese medicine, in improving the kidney function and strengthening bone. Twenty‐one Wistar rats were divided into three groups: control group (rats before prednisolone inducing), a model group (prednisolone‐induced group) and a treatment group (prednisolone‐induced rats that were then administered RD ethanol extracts). By using pattern recognition analysis, a significant change in the metabolic profile of kidney tissue samples was observed in the model group and restoration of the profile was observed after the administration of RD ethanol extracts. Some significantly changed biomarkers related to osteoporosis such as sphingolipids (C16 dihydrosphingosine, C18 dihydrosphingosine, C18 phytosphingosine, C20 phytosphingosine), lysophosphatidycholines (C16:0 LPC, C18:0 LPC) and phenylalanine were identified. As a complement to the metabolic profiling of RD in plasma, these biomarkers suggest that kidney damage, cell cytotoxicity and apoptosis exist in osteoporosis rats, which is helpful in further understanding the underlying process of glucocorticoid‐induced osetoporosis and the suggested therapeutic effects of RD. The method shows that tissue target metabonomics might provide a powerful tool to further understand the process of disease and the mechanism of therapeutic effect of Chinese medicines. Copyright © 2014 John Wiley & Sons, Ltd.
Determination of 4‐hydroxy‐2‐nonenal in serum by high‐performance liquid chromatography with fluorescence detection after pre‐column derivatization using 4‐(N,N‐dimethylaminosulfonyl)‐7‐hydrazino‐2,1,3‐benzoxadiazole
Authors: Imazato Takahiro , Shiokawa Akina , Kurose Yuri , Katou Yasuha , Kishikawa Naoya , Ohyama Kaname , Ali Marwa Fathy Bakr , Ueki Yukitaka , Maehata Eisuke , Kuroda Naotaka 
Published: May 2014

ABSTRACT4‐Hydroxy‐2‐nonenal (4HNE) is a major aldehyde generated during lipid peroxidation. The clinical monitoring of 4HNE in biological fluids could be useful for the early diagnosis of several diseases involving lipid peroxidation, such as rheumatoid arthritis, Parkinson's disease and cancer. In this study, an HPLC with fluorescence detection method was developed for the determination of 4HNE in human serum. The proposed method involves the extraction of 4HNE from human serum by subzero temperature extraction and fluorescent labeling of 4HNE with 4‐(N,N‐dimethylaminosulfonyl)‐7‐hydrazino‐2,1,3‐benzoxadiazole. The lower detection limit (signal‐to‐noise ratio = 3) of the method was 0.06 µm in serum. The proposed method was successfully applied to the measurement of 4HNE in sera obtained from patients with rheumatoid arthritis. Copyright © 2014 John Wiley & Sons, Ltd.
Preparation of lysozyme molecularly imprinted polymers and purification of lysozyme from egg white
Authors: Wang Xuejiao , Dong Shaohua , Bai Quan 
Published: May 2014

ABSTRACTMolecular imprinting as a promising and facile separation technique has received much attention because of its high selectivity for target molecules. In this study, lysozyme molecularly imprinted polymers (Lys‐MIPs) were successfully prepared by the entrapment method with lysozyme as the template molecule, acrylamide as the functional monomer and N,N‐methylenebisacrylamide as the cross‐linker. The removal of the template lysozyme from the molecularly imprinted polymers was investigated in detail by two methods. The synthesized Lys‐MIPs were characterized by scanning electron microscopy and Fourier transform–infrared, and the adsorption capacity, selectivity and reproducibility of the Lys‐MIPs were also evaluated. The maximum adsorption capacity reached 94.8 mg/g, which is twice that of nonmolecularly imprinted polymers, and satisfactory selectivity and reproducibility were achieved. Using the Lys‐MIP column, lysozyme could be separated completely from egg white, with purity close to 100% and mass recovery of 98.2%. This illustrated that the synthesized Lys‐MIPs had high specific recognition and selectivity to the template lysozyme when they were applied to a mixture of protein standards and a real sample. Copyright © 2014 John Wiley & Sons, Ltd.
Improved analysis of vitamin D metabolites in plasma using liquid chromatography tandem mass spectrometry, and its application to cardiovascular research
Authors: Sandhu Jatinderpal K. , Auluck Janica , Ng Leong L. , Jones Donald J. L. 
Published: May 2014

ABSTRACTThe accurate and specific measurement of vitamin D is increasingly important for determining the role of vitamin D in the pathogenesis of disease. Liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS) has increasingly become the analytical modality of choice for the analysis of vitamin D. There are many advantages to using LC/MS/MS, such as high specificity and sensitivity to help distinguish the isomers of vitamin D. This rapid method, modified from a Waters Corporation application note, consists of minimal sample manipulation using liquid–liquid extraction and incorporates an internal standard. The supernatant is dried down and injected onto an ultra‐high‐performance liquid chromatography/electrospray ionization tandem mass spectrometer. The total analysis time is 10 min per injection, enabling high throughput of samples. This method also incorporates two commercial quality control standards to provide a robust system with acceptable coefficient of variation. The analysis of control and heart failure plasma samples showed significant differences in the levels of vitamin D3 between these two groups; however, in the control group, there were individuals who were vitamin D deficient. Overall, the vitamin D3 levels were higher in control samples than in heart failure individuals. As expected, vitamin D2 levels were not observed in many of the samples analysed. This modified method is quick and incorporates an internal standard to allow for any loss in the extraction procedure. The method also includes quality control samples to enable assay standardization. The assay involves inexpensive pre‐sample clean‐up, aiding high throughput, which is important in many laboratories. Copyright © 2014 John Wiley & Sons, Ltd.
Emerging K1 serotype Klebsiella pneumoniae primary liver abscess: three cases presenting to a single university hospital in Norway
Authors: Holmås Kristoffer , Fostervold Aasmund , Stahlhut Steen Gustav , Struve Carsten , Holter Jan Cato 
Published: May 2014

Key Clinical MessageCommunity‐acquired Klebsiella pneumoniae primary liver abscess (KLA) has been emerging worldwide over the past two decades and with high incidence in Asia. The presence of specific virulence characteristics is a risk factor for a syndrome with metastatic complications. This report signals an increasing emergence in Northern Europe.
Fe and Mn‐Based Catalysts Supported on γ‐Al2O3 for CO Oxidation under O2‐Rich Conditions
Authors: Tepluchin Marina , Casapu Maria , Boubnov Alexey , Lichtenberg Henning , Wang Di , Kureti Sven , Grunwaldt Jan‐Dierk 
Published: May 2014

AbstractMnOx and FeOx‐based catalysts supported on γ‐Al2O3 (0.1–20 wt %) were prepared by using two methods: incipient wetness impregnation and single‐step flame spray pyrolysis. The effect of the structural properties and composition on the CO oxidation activity was systematically evaluated and correlated with the preparation methods. The characterization of the samples by XRD, X‐ray absorption spectroscopy, TEM, and temperature‐programmed reduction by hydrogen revealed that, in contrast to the use of incipient wetness impregnation, flame spray pyrolysis leads to the formation of highly dispersed homogeneously distributed FeOx and MnOx species. A partial incorporation of Fe and Mn ions into the γ‐Al2O3 lattice for low metal oxide loadings and for samples prepared by flame spray pyrolysis was observed. In general, the CO oxidation activity increased with the transition metal oxide loading. Below 200 °C, Mn‐based catalysts demonstrated the highest catalytic performance. However, the addition of water decreased the performance, especially at lower temperatures, which demonstrates the competitive adsorption on the active sites. The presence of NO had no effect on the CO conversion. A significant effect of the preparation method on the catalytic performance was observed during hydrothermal aging. The superior distribution of the active species obtained by flame spray pyrolysis leads to thermally more stable catalysts.
On the Nature of Blueshifting Hydrogen Bonds
Authors: Mo Yirong , Wang Changwei , Guan Liangyu , Braïda Benoît , Hiberty Philippe C. , Wu Wei 
Published: May 2014

AbstractThe block‐localized wave function (BLW) method can derive the energetic, geometrical, and spectral changes with the deactivation of electron delocalization, and thus provide a unique way to elucidate the origin of improper, blueshifting hydrogen bonds versus proper, redshifting hydrogen bonds. A detailed analysis of the interactions of F3CH with NH3and OH2shows that blueshifting is a long‐range phenomenon. Since among the various energy components contributing to hydrogen bonds, only the electrostatic interaction has long‐range characteristics, we conclude that the contraction and blueshifting of a hydrogen bond is largely caused by electrostatic interactions. On the other hand, lengthening and redshifting is primarily due to the short‐rangen(Y)→σ*(XH) hyperconjugation. The competition between these two opposing factors determines the final frequency change direction, for example, redshifting in F3CH⋅⋅⋅NH3and blueshifting in F3CH⋅⋅⋅OH2. This mechanism works well in the series FnCl3−nCH⋅⋅⋅Y (n=0–3, Y=NH3, OH2, SH2) and other systems. One exception is the complex of water and benzene. We observe the lengthening and redshifting of the OH bond of water even with the electron transfer between benzene and water completely quenched. A distance‐dependent analysis for this system reveals that the long‐range electrostatic interaction is again responsible for the initial lengthening and redshifting.
Multifunctional 8‐Hydroxyquinoline‐Appended Cyclodextrins as New Inhibitors of Metal‐Induced Protein Aggregation
Authors: Oliveri Valentina , Attanasio Francesco , Puglisi Antonino , Spencer John , Sgarlata Carmelo , Vecchio Graziella 
Published: May 2014

AbstractMounting evidence suggests a pivotal role of metal imbalances in protein misfolding and amyloid diseases. As such, metal ions represent a promising therapeutic target. In this context, the synthesis of chelators that also contain complementary functionalities to combat the multifactorial nature of neurodegenerative diseases is a highly topical issue. We report two new 8‐hydroxyquinoline‐appended cyclodextrins and highlight their multifunctional properties, including their CuII and ZnII binding abilities, and capacity to act as antioxidants and metal‐induced antiaggregants. In particular, the latter property has been applied in the development of an effective assay that exploits the formation of amyloid fibrils when β‐lactoglobulin A is heated in the presence of metal ions.
Copper‐Catalyzed Aerobic Oxidative NS Bond Functionalization for CS Bond Formation: Regio‐ and Stereoselective Synthesis of Sulfones and Thioethers
Authors: Li Xianwei , Xu Yanli , Wu Wanqing , Jiang Chang , Qi Chaorong , Jiang Huanfeng 
Published: May 2014

AbstractA regio‐ and stereoselective synthesis of sulfones and thioethers by means of CuI‐catalyzed aerobic oxidative NS bond cleavage of sulfonyl hydrazides, followed by cross‐coupling reactions with alkenes and aromatic compounds to form the CS bond, is described herein. N2 and H2O are the byproducts of this transformation, thus offering an environmentally benign process with a wide range of potential applications in organic synthesis and medicinal chemistry.
Diazepinoporphyrazines Containing Peripheral Styryl Substituents and Their Promising Nanomolar Photodynamic Activity against Oral Cancer Cells in Liposomal Formulations
Authors: Piskorz Jaroslaw , Konopka Krystyna , Düzgüneş Nejat , Gdaniec Zofia , Mielcarek Jadwiga , Goslinski Tomasz 
Published: May 2014

AbstractThe photochemical properties and photodynamic activity of three porphyrazines (Pzs) containing annulated diazepine rings, including novel demetalated porphyrazine‐possessing bis(styryl)diazepine moieties were investigated. The porphyrazines were evaluated in terms of their electronic absorption and emission properties, their tendency to undergo aggregation and photodegradation, as well as their singlet oxygen generation efficiency. The in vitro photodynamic activity of the porphyrazines and their liposomal formulations were examined by using two oral squamous cell carcinoma cell lines. Magnesium(II) tribenzodiazepinoporphyrazine (1) revealed the highest phototoxic effect in both cell lines used, H413 and HSC‐3. Encapsulation of Pz 1 into L‐α‐phosphatidyl‐d,l‐glycerol:1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine liposomes resulted in a nearly threefold increase in photocytotoxicity relative to that of the solution of Pz 1 (IC50 values of 45 and 129 nM, respectively).
Speed Dependence of Thermochemical Nanolithography for Gray‐Scale Patterning
Authors: Carroll Keith M. , Desai Maitri , Giordano Anthony J. , Scrimgeour Jan , King William P. , Riedo Elisa , Curtis Jennifer E. 
Published: May 2014

AbstractThermochemical nanolithography (TCNL) is a high‐resolution lithographic technique and, owing to its fast speed, versatility, and unique ability to fabricate arbitrary, gray‐scale nanopatterns, this scanning probe technique is relevant both for fundamental scientific research as well as for nanomanufacturing applications. In this work, we study the dependence of the TCNL driven chemical reactions on the translation speed of the thermal cantilever. The experimental data compares well with a model of the chemical kinetics for a first‐order reaction. The impact of higher order reactions on the optimization of TCNL is addressed. The reported quantitative description of the speed dependence of TCNL is exploited and illustrated by fabricating controlled gradients of chemical concentration.
Primary cilium and sonic hedgehog signaling during neural tube patterning: Role of GPCRs and second messengers
Authors: Pal Kasturi , Mukhopadhyay Saikat 
Published: May 2014

ABSTRACTThe ventral neural tube in vertebrates is patterned by a gradient of sonic hedgehog (Shh) secreted from the notochord and floor plate. Forward genetic screens first pointed to the role of the primary cilium in ventral neural tube patterning. Further research has shown that most components of the Shh pathway localize to or shuttle through the primary cilium. In the absence of Shh, the bifunctional Gli transcription factors are proteolytically processed into repressor forms in a protein kinase A (PKA)‐ and cilium‐dependent manner. Recent work suggests that the orphan G‐protein‐coupled receptor (GPCR) Gpr161 localizes to cilia, and functions as a negative regulator of Shh signaling by determining Gli processing via cAMP signaling. The primary cilium also functions as a signaling compartment for calcium in the Shh pathway. A better understanding of the role of the cilium as a signaling compartment, and the interplay of second messenger systems that regulate PKA activation and Gli amplification during signaling is critical for deciphering the role of Shh during development, neuronal differentiation, and tumorigenesis. © 2014 Wiley Periodicals, Inc. Develop Neurobiol 75: 337–348, 2015
The loss of control over eating scale: Development and psychometric evaluation
Authors: Latner Janet D. , Mond Jonathan M. , Kelly Mackenzie C. , Haynes Stephen N. , Hay Phillipa J. 
Published: May 2014

ABSTRACTObjectiveThis study describes the development, content validity, and convergent validity of the Loss of Control over Eating Scale (LOCES).MethodAn initial pool of 56 items covering 13 facets of loss‐of‐control eating was assembled by reviewing qualitative literature, clinical descriptions, and research on binge eating. Eating disorder experts (n = 34) and eating disorder clients (n = 22) rated each proposed item's clarity and relevance to the construct of loss‐of‐control eating, rated 13 facets for their relevance to the construct, and provided open‐ended feedback about the items and facets. Based on the experts' and clients' quantitative and qualitative feedback, scale items were clarified, 28 items were added, and 10 were deleted. University students (n = 476; 70% female, mean age = 20.4 years) completed the resulting 74‐item questionnaire, rating how often they had the experience identified in the item while eating in the last 4 weeks. They also completed the measures of eating disturbance, general distress, functional impairment, and general self‐control.ResultsThe resulting 24‐item LOCES (Cronbach's α = .96) retained items with highest item–total correlations and coverage of the 12 construct facets that experts rated as important. The LOCES was significantly correlated with eating disturbances, general distress, functional impairment, and general self‐control. Three subfactors were identified: behavioral, cognitive/dissociative, and positive/euphoric aspects of loss‐of‐control eating. A brief, seven‐item version of the LOCES was developed and validated.DiscussionA thorough process of development, content validation, and psychometric evaluation in multiple samples yielded the multifaceted LOCES and its brief form. These instruments may be useful in assessing loss‐of‐control eating in both clinical and nonclinical settings. © 2014 Wiley Periodicals, Inc. (Int J Eat Disord 2014; 47:647–659)
Obstetric complications and eating disorders: A replication study
Authors: Tenconi Elena , Santonastaso Paolo , Monaco Francesco , Favaro Angela 
Published: May 2014

ABSTRACTObjectiveTo provide data about the role of obstetric complications (OCs) in a large and well‐characterized sample of patients with anorexia nervosa (AN) or bulimia nervosa (BN).MethodThe new sample consists of 150 patients with AN and 35 patients with BN, and 73 healthy women; statistical analyses were performed on the new sample alone and on the larger sample created by merging the new dataset with the previous one (264 AN, 108 BN, and 624 healthy women). All data about OCs were collected blind to diagnostic status.ResultsOC rates in the replication sample were similar to those of our previous studies. In the whole sample, the risk of developing AN was significantly associated with the occurrence and number of pregnancy, delivery, hypoxic, and dysmaturity complications. The risk of developing BN was significantly associated with dysmaturity complications. Signs of retarded fetal growth (being small and short for gestational age, short head circumference) significantly distinguished BN patients from both AN and healthy individuals. Significantly higher number of OCs were found in the binge eating/purging type of AN, in comparison with restricting AN patients.DiscussionOur study provides further evidence of the role of OCs as putative risk factors for the development of eating disorders, showing different pathways between AN and BN. © 2014 Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:424–430)