bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telerehabilitation using a 2-D planar arm rehabilitation robot for hemiparetic stroke: A feasibility study of clinic-to-home exergaming therapy
Authors: Ollinger Gabriel Aguirre , Chua Karen Sui Geok , Ong Poo Lee , Kuah Christopher Wee Keong , Plunkett Tegan Kate , Ng Chwee Yin , Khin Lay Wai , Goh Kim Huat , Chong Wei Binh , Low Jaclyn Ai Mei , Mushtaq Malaika , Samkharadze Tengiz , Kager Simone , Cheng Hsiao-Ju , Hussain Asif 
Published: August 2024

Abstract

Aims: We evaluated the feasibility, safety and efficacy of a 2D-planar robot for minimally-supervised home-based upper-limb therapy for post-stroke hemiparesis.
Materials and Methods: The H-Man, end effector robot, combined with web-based software application for remote tele-monitoring were evaluated at homes of participants. Inclusion criteria were: strokes > 28 days, Fugl-Meyer Motor Assessment (FMA) >10-60/66, presence of a carer and absence of medical contraindications. Participants performed self-directed, minimally-supervised H-Man training for 30 consecutive days at their homes, after 2 therapist-supervised clinic on-boarding sessions. Web-based compliance measures were accessed sessions (>20mins/day), training minutes/day and active training hours/30days. Clinical outcomes obtained at weeks 0, 5 (post-training), 12 and 24 (follow-up) consisted of FMA, Action Research Arm Test (ARAT) and WHO-Stroke Specific Quality Of Life (SSQOL). To estimate immediate economic benefits of the home-based robotic therapy, we performed cost-effectiveness analysis (CEA), followed by budget impact analysis (BIA).
Results: Altogether, all 12 participants completed H-Man at home without adverse events; 9 (75.0%) were males, mean (SD) age, 59.4 years (9.5), median (IQR) stroke duration 38.6 weeks (25.4, 79.6) baseline FMA (0-66) 42.1 ±13.2, ARAT (0-57) 25.4 ±19.5, SSQOL (0-245) 185.3 ±32.8. At week 5 follow-up, mean (SD) accessed days were 26.3 days ±6.4, active training hours of 35.3 hours ±14.7/30days, or ~6 days/week and 77 training minutes ± 20.9/day were observed. Significant gains were observed from baseline across time; ΔFMA 2.4 at week 5 (FMA 44.5, CI 95% 39.7 – 49.3, p < 0.05) and ΔFMA 3.7 at week 24 (FMA 45.8, CI 95% 40.5 – 51, p < 0.05); ΔARAT 2.6 at week 5 (ARAT 28.0, CI 95% 19.3 – 36.7, p < 0.05), and ΔARAT 4.8 at week 24 (ARAT 30.2, CI 95% 21.2 – 39.1, p < 0.05). At week 5 follow-up, 91% of participants rated their overall experience as satisfied or very satisfied. Incremental CEA observed savings of -S$144/per cure over 24 weeks, BIA – potentially 12% impact reduction over five years.
Conclusions: This study demonstrates the feasibility, acceptability, safety, clinical efficacy and cost-effectiveness of a home-based, web-enabled telemonitored carer-supervised robotics-aided therapy.
Parenting style in the NICU is stable, measurable, and predictive of 2-year parenting style
Authors: Neel Mary Lauren , Kjeldsen Caitlin , Srini Rachelle , McGovern Kevin , He Zhulin , Maitre Nathalie 
Published: August 2024

Abstract

Objective: To establish psychometric properties of Baby Care Questionnaire (BCQ) in preterm infants, individual level stability of BCQ scores from NICU to 2 years corrected gestational age (CGA), and to determine whether early BCQ scores predict 2-year parenting styles.
Study design: In this prospective study, the BCQ assessed early parental structure and attunement at 4 time points between NICU and 2-years CGA. The Parenting Styles and Dimensions Questionnaire (PSDQ) at 2-years evaluated authoritative, authoritarian, and permissive parenting styles. Intraclass correlations analyzed reliability and Reliable Change Index (RCI) studied individual-level stability. Multivariate regression examined predictive properties.
Results: n=162 parent/preterm infant dyads were followed sequentially. Cronbach’s α BCQ structure and attunement were 0.9 and 0.8. RCI showed high individual level stability of both constructs. Higher early structure scores were associated higher 2-year authoritative scores.
Conclusions: Early parenting characteristics beginning in the NICU are stable in infancy, measurable, reliable, and predictive of 2-year parenting styles. Early parental structure correlates with 2-year authoritative parenting style.
Dynamicity of Ecosystem Service Value Driven by Land Use/Land Cover Alteration in Vadodara City, India
Authors: Pal Anindita , Tiwari Atul K , Dutta Shyamal , Rawal Ami , Kanchan Rolee 
Published: August 2024

Abstract

The ecosystem is considered the fundamental unit of ecology which plays a crucial role in providing a range of essential services to individuals. These services include the provision of food and shelter, as well as the regulation of climate and environment, either directly or indirectly. However, the emergence of urban industrial cities has witnessed the uncontrolled exploitation of resources, the decline of biodiversity, unprecedented population growth and climate change. Consequently, these factors have led to ecological crises as the ecosystem services experience a gradual increase in the built-up areas. Considering this, the current study examines the relationship between land use change and the status of ecosystem services in Vadodara City, India. The ecosystem service value was calculated using the methodology proposed by Costanza in 1997 and 2014, while the contribution of Land Use/Land Cover (LULC) was determined using the Maximum Likelihood Classification. The findings highlighted that LULCs are critical drivers for the loss of ecosystem services. During 2001–2021, it was found that built-up and water bodies increased by 24.48% and 0.21%, respectively, while vegetation, agricultural and barren land decreased by 1.11%, 25.61% and 0.19% respectively. The valuation of ecosystem services in 2001 observed $3,517,118 and $125,607,186 using the corresponding coefficients of Costanza, 1997 and 2014, while in 2021, their cumulative value slightly increased to $3,629,024 based on the 1997 coefficient and $131,537,398 based on the 2014 coefficient. Furthermore, the study analyses the response of ecosystem service values to land use alterations and the elasticity value at the zonal level to gain insight into spatial variation.
Chondroblastoma: A Sharing of Therapeutic Experiences
Authors: yang Di , Ouyang Haiping , zhou Ziyu , Wang Zhongliang 
Published: August 2024

Abstract

Background
Chondroblastoma (CBL) is a rare benign bone tumor with potentially local recurrence and metastases. At present, the local recurrence risk factors are controversial. The purpose of this study is to analysis local recurrence factors in our medical centre. Sharing Our Therapeutic Experience.
Methods
In a retrospective study, we identified 28 patients who were diagnosed pathologically and an analysis that included age at diagnosis, gender, side, tumor location, tumor volume, epiphyseal plate status, affected epiphyseal plate, aneurysmal bone cyst, surgical therapy was performed. Kaplan-Meier estimators were used to analyze the local recurrence-free survival (LRFS). Intraclass Correlation Coefficient (ICC) and Kappa coefficient were utilized for the analysis of inter-rater variability in medical evaluations.
Results
All relapsed patients were over the 11 years old. There was a statistically significant difference in tumor volume between patients with recurrence and those without recurrence, as determined by the unpaired t-test. Furthermore, the Kaplan-Meier curve revealed a statistically significant association between local recurrence-free survival and tumor volume, with a distinct separation observed between patients with tumor volumes greater than 25 cm³ and those with volumes less than 25 cm³.
Conclusion
Curettage, combined with alcohol as an adjuvant and bone grafting, has demonstrated promising outcomes. However, it is noteworthy that a larger tumor size correlates with an increased likelihood of recurrence.
A risk signature constructed by Tregs-related genes predict the clinical outcomes and immune therapeutic response in kidney cancer
Authors: Li Gang , Cui Jingmin , Li Tao , Li Wenhan , Chen Peilin 
Published: August 2024

Abstract

Regulatory T cells (Tregs) have been found to be related to immune therapeutic resistance in kidney cancer. However, the potential Tregs-related genes still need to be explored. Our study found that patients with high Tregs activity show poor prognosis. Through co-expression and differential expression analysis, we screened several Tregs-related genes (KTRGs) in kidney renal clear cell carcinoma. We further conducted the univariate Cox regression analysis and determined the prognosis-related KTRGs. Through the machine learning algorithm - Boruta, the potentially important KTRGs were screened further and submitted to construct a risk model. The risk model could predict the prognosis of RCC patients well, high risk patients show a poorer outcomes than low risk patients. Multivariate Cox regression analysis reveals that risk score is an independent prognostic factor. Then, the nomogram model based on KTRG risk score and other clinical variables was further established, which shows a high predicted accuracy and clinical benefit based on model validation methods. In addition, we found EMT, JAK/STAT3, and immune-related pathways highly enriched in high risk groups, while metabolism-related pathways show a low enrichment. Through analyzing two other external immune therapeutic datasets, we found that the risk score could predict the patient's immune therapeutic response. High-risk groups represent a worse therapeutic response than low-risk groups. In summary, we identified several Tregs-related genes and constructed a risk model to predict prognosis and immune therapeutic response. We hope these organized data can provide a theoretical basis for exploring potential Tregs' targets to synergize the immune therapy for RCC patients.
Investigating Factors Influencing Subjective Taste and Smell Alterations in Colorectal Cancer Patients
Authors: Wang Wenhui , Li Zhao , Zhang Xiaomeng , Li Yize , Kong Shengnan , Sun Juanhua , Zhang Hongmei 
Published: August 2024

Abstract

Purpose
This study aimed to assess the factors influencing self-reported taste and smell disturbances in patients with colorectal cancer.
Methods
A total of 152 participants were enrolled in the study. All subjects completed surveys that assessed taste and smell alterations using the Taste and Smell Survey (TSS), linear analog self-assessment (LASA), Patient-Generated Subjective Global Assessment (PG-SGA), and Pittsburgh Sleep Quality Index (PSQI). The TSS gathered data on the nature of taste and smell changes and their impact on quality of life. LASA was employed to evaluate the quality of life in cancer patients, while PG-SGA was used to indicate the nutritional status of the participants. PSQI was used to evaluate sleep quality.
Results
Among the 152 colorectal cancer patients, the total TSS score was 5.68 ± 2.84. No changes were observed in 5 cases (3.3%), mild changes in 58 cases (38.2%), and moderate to severe changes in 89 cases (58.6%). The severity of taste and smell disturbances increased with tumor stage, the cycle of chemotherapy, deteriorating sleep quality, and poor nutritional status, displaying significant correlations. Additionally, 83 patients (54.6%) complained of taste abnormalities, while 81 (53.3%) reported smell abnormalities.
Conclusion
Taste and smell disturbances are prominent in colorectal cancer patients. Taste abnormalities are significantly correlated with tumor stage, the cycle of chemotherapy, sleep quality, and nutritional status. Recognizing the high prevalence of taste and smell abnormalities and the influencing factors among patients with colorectal cancer may aid in predicting and understanding the severity of these symptoms more effectively.
Exploring the Molecular Mechanisms and Shared Genetic Characteristics of Monoclonal Gammopathy of Undetermined Significance and Multiple Myeloma
Authors: Fu Yuanjing , Gu Weiying , Lin Yan , Cao Yang , Luo Jiaru , Zheng Zhuojun , Liu Yan 
Published: August 2024

Abstract

Monoclonal gammopathy of undetermined significance (MGUS) serves as a precursor to multiple myeloma (MM), with a subset of MGUS cases advancing to MM each year. Despite extensive research, the mechanisms driving this progression are not yet fully understood. Leveraging transcriptomic data from the Gene Expression Omnibus (GEO) database, this study conducted an analysis of differentially expressed genes (DEGs) between MGUS and MM. Weighted gene co-expression network analysis (WGCNA) was utilized to pinpoint significant gene modules linked to disease progression. Univariate Cox analysis and LASSO regression were employed to identify genes associated with MM prognosis. Immune scores and immune cell proportions were calculated using ESTIMATE and CIBERSORT tools. A total of 961 DEGs related to MM and 355 DEGs associated with MGUS were identified. Cross-analysis revealed 12 intersecting genes, with four (DAP3, HIST1H1C, MRPL4, and UBE2S) as core genes. The MGUSscore effectively stratified MM patients into high-risk and low-risk groups, with the high-risk group showing significantly shorter overall survival (P < 0.05). Core genes were closely linked to immune cell infiltration. A ceRNA network identified 68 miRNAs and 10 lncRNAs related to core genes. DAP3, HIST1H1C, MRPL4, and UBE2S are potential therapeutic targets for MM.
Bio-printing method as a novel approach to obtain a fibrin scaffold settled by limbal epithelial cells for corneal regeneration
Authors: Pietryga Krzysztof , Jesse Katarzyna , Drzyzga Rafał , Konka Adam , Zembala-John Joanna , Kowalik Aleksandra , Kiełbowicz Zdzisław , Ćwirko Marek , Bułdak Rafał J. , Dobrowolski Dariusz , Wylęgała Edward 
Published: August 2024

Abstract

Treatment of Limbal Stem Cell Deficiency (LSCD), based on autologous transplantation of the patient's stem cells, is one of the few medical stem cell therapies approved by the European Medicines Agency (EMA). It relies on isolating and culturing in vivo Limbal Epithelial Stem Cells (LESC) and then populating them on the fibrin substrate, creating a scaffold for corneal epithelial regeneration. Such a solution is then implanted into the patient's eye. The epithelial cell culture process is specific, and its results strongly depend on the initial cell seeding density. Achieving control of the density and repeatability of the process is a desirable aim and can contribute to the success of the therapy. The study aimed to test 3D printing as a potential technique to increase the control over LESCs seeding on a scaffold and improve process reproducibility. Cells were applied to 0.5 mm thick, flat, transparent fibrin substrates using extrusion bioprinting; the control was the traditional manual application of cells using a pipette. The 3D printing enabled uniform coverage of the scaffold surface, and LESCs density in printed lines was close to the targeted value. Moreover, printed cells had higher cell viability than those seeded traditionally (91.1 ± 8.2% vs 82.6 ± 12.8%). The growth rate of the epithelium was higher in 3D-printed samples. In both methods, the epithelium had favorable phenotypic features (p63 + and CK14+). 3D printing constitutes a promising approach in LSCD therapy. It provides favorable conditions for LESCs growth and process reproducibility. Its application may lead to reduced cell requirements, thereby to using fewer cells on lower passages, which will contribute to preserving LESCs proliferative potential.
Effect of Instructional Guidelines on the Parent's Care of Neonates with Neural Tube Defects
Authors: Khamis Asmaa 
Published: August 2024

Abstract

Background Neural Tube Defects account for the most congenital anomalies of the central nervous system for paediatric patients. The presence or diagnosis of any congenital anomalies, especially neural tube defects can have a significant impact on parents holistically. This study aimed to evaluate the effect of instructional guidelines on parents’ care for neonates with neural tube defects.
Methods: A quasi-experimental design was used to conduct this study. This study was conducted in the neonatal intensive care unit and the Neurological Department at the Children's Hospital affiliated to Ain Shams University Hospital and Helwan General Hospital. A convenience sample consisting of 40 parents and their neonates who suffered from neural tube defects was used.
Results: There was a marked improvement in parents’ knowledge and reported practices as well as the stress level after implementation of the instructional guidelines. There were strong positive correlations between studied parents’ total knowledge and their total reported practices related to their care for their children with neural tube defects. There was a statistically significant relationship between the gender of the studied parents and their knowledge, practices and PSI regarding the care of neonates with Neural Tube defects post-implementation of instructional guidelines (P = 0.000, P ≤ 0.001).
Conclusion: The implementation of the instructional guidelines has a positive effect on parents’ care for neonates with neural tube defects, so the research findings support the research hypothesis.
Characterization and Application of the Fluorescent APTA-nano Biosensors Based on CdTe-TGA QDs for Determination of 25-hydroxyvitamin D3
Authors: Bardajee Ghasem Rezanejade , Tahriri Farhad , Vancaeyzeele Cedric 
Published: August 2024

Abstract

Medical studies have shown that vitamin D deficiency is strongly associated with several metabolic disorders, including diabetes, cardiovascular diseases, and cancer. So, it is crucial to regularly check its concentration in the blood serum. Traditional methods for detecting 25-hydroxyvitamin D3 [25(OH)D3] as a marker of vitamin D status are expensive, time-consuming, and require a skilled workforce and specialized laboratory. This study developed a simple and cost-effective fluorescence system for 25-hydroxyvitamin D3 determination. The fluorescent APTA-nano biosensors were fabricated using cadmium telluride quantum dots modified with thioglycolic acid (CdTe-TGA QDs) and functionalized with thiol-25(OH)D3-aptamer through ligand exchange. The thiol-25(OH)D3-aptamer interacted directly with CdTe-TGA QDs, increasing fluorescence intensity. However, it decreased when the target molecules of 25-hydroxyvitamin D3 were introduced. The structural and morphological characteristics of APTA-nano biosensors were confirmed by various analytical methods such as UV-visible spectroscopy, Fourier-transform infrared spectroscopy (FT-IR), field emission scanning electron microscopy (FESEM), energy dispersive x-ray spectroscopy (EDX), transmission electron microscopy (TEM), and dynamic light scattering (DLS). According to the typical Stern-Volmer equation, the relationship between fluorescent quenching and target concentration was linear with a detection limit of 1.35 × 10-8 M, a quantification limit of 4.50 × 10-8 M, and a relative standard deviation of 1.75%. The optimized APTA-nano biosensor demonstrated high specificity towards the target and stability over 28 days. Furthermore, it detected 25-hydroxyvitamin D3 in human serum with a recovery rate of up to 99.77%. The results indicate that the fluorescent APTA-nano biosensors could be valuable in developing robust sensing technology for low-concentrated analytes.
Methionyl-tRNA synthetase 1 expression, the possibility as a diagnostic and prognostic factor in papillary thyroid cancer
Authors: Park Seulkee , Lee Jun Sung , Jeong Ho Jung , Yun Hyeok Jun , Kim Seok-Mo , Nahm Ji Hae , Chang Hojin , Lee Yong Sang , Chang Hang-Seok 
Published: August 2024

Abstract

Background
Methionyl-tRNA synthetase 1 (MARS1) is a critical enzyme in translation initiation, responsible for catalyzing the transfer of Met to the initiator tRNA. In this study, we aimed to examine whether MARS1 expression is different between normal follicular cells and papillary thyroid carcinoma (PTC) cells in thyroid tissue and whether it can supplement the limitations of general cell staining methods currently performed for diagnosis of PTC.
Methods
Initially, 103 patients were included to compare MARS1 expression in PTC and normal follicular cells. Next, 100 patients were selected to compare MARS1 expression using immunohistochemical analysis in patients with (n = 50) and without (n = 50) lateral neck metastasis.
Results
The average MARS1 expression grade of PTC cells was 2.59 and that of normal follicular cells was 1.28. MARS1 expression in the two groups showed significant differences (p < 0.001). There was a significant difference in the average MARS1 expression grade of PTC cells between the metastasis and non-metastasis groups (p < 0.05). Additionally, a significant difference was observed in the average MARS1 expression grade between the lymph node of the metastasis group and PTC cells of the non-metastasis group (p < 0.05).
Conclusions
Our analyses suggest that MARS1 could be used as a complementary method to the current fine needle aspiration biopsy tissue staining method. Additionally, MARS1 could be a predictor of the prognosis of PTC.
The efficacy of mifepristone-misoprostol regimen versus misoprostol only for medication abortion at 22+0/7 – 30+0/7 weeks gestational duration
Authors: Touval Or , Ellert Avital , Daykan Yair , Schonman Ron , Klein Zvi , Yagur Yael 
Published: August 2024

Abstract

Objective
To compare duration of medication abortion after pretreatment with mifepristone versus misoprostol-only regimens at 22 + 0/7 to 30 + 0/7 weeks gestational duration.
Methods
This retrospective cohort study included patients admitted for medication abortion from 2014 to 2022. Included patients underwent feticide due to genetic or anatomical abnormalities at gestational duration of 22 + 0/7 to 30 + 0/7 weeks. Excluded from the study were patients admitted at gestational duration of < 22 + 0/7 or > 30 + 0/7 weeks, multiple gestation, diagnosis of intrauterine fetal demise before feticide, with contraindication for vaginal delivery and who were administered a medical regimen other than the mifepristone-misoprostol or misoprostol-only protocol. Information collected included patients’ demographics, clinical outcome, additional procedural interventions, and complications. Data of patients treated with mifepristone-misoprostol versus misoprostol-only were compared.
Results
The study group included 46 patients in the mifepristone-misoprostol group and 35 in the misoprostol-only group. Median interval from first dose of misoprostol to fetal expulsion was shorter in the mifepristone-misoprostol group (10.6 vs. 15.3 h; p = 0.007) with shorter duration of hospitalization (3.5 ± 1.1 vs. 4.1 ± 1.2 days; p = 0.013). Study groups were no different in terms of complications. Patients in the mifepristone group had an earlier gestational duration (23.8 ± 1.69 vs. 25.37 ± 2.4 weeks; p = 0.002). However, multivariable Cox regression found that mifepristone-misoprostol was independently associated with shorter induction (OR 1.7, 95% CI 1.03–2.9, p = 0.03,).
Conclusion
medication abortion with mifepristone-misoprostol was associated with shorter time to fetal expulsion at 22 + 0/7 to 30 + 0/7 weeks gestational duration, compared with misoprostol-only regimen.