bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the trend of mortality of genitourinary system cancers in Babol, North Iran (2013–2021)
Authors: Ebrahimi Pouyan , Karami Mohsen , Hosseini-Berneti Seyed-Hossein , Lashkarbolouki Amir-Hossein , Keshtegar Sana , Ghezel Mohammad-Amin , Nikbakht Hossein-Ali 
Published: July 2024

Abstract
Background
Cancers of the genitourinary system, particularly prostate, bladder, and kidney cancer, exhibit a high prevalence. Consequently, predicting the morbidity and mortality of genitourinary cancers holds great significance for future planning and implementation. This study aimed to examine the crude and age-standardized rates of mortality and the trend of genitourinary cancers over nine years in northern Iran.

Methods
This cross-sectional study used data on the number of deaths attributed to genitourinary cancers recorded in Babol City between 2013 and 2021 through the cause of death registration and classification system. Population estimates were derived from the latest census reports. Subsequently, crude and age-standardized rates, as well as trends for genitourinary cancers, were calculated.

Results
A total of 307 deaths occurred, with an average age of 75.6 ± 14.3 years due to genitourinary cancers. The crude and age-standardized rates of genitourinary cancers increased from 2.7 and 1.9 per hundred thousand people in 2013 to 7.7 and 5.9 per hundred thousand people in 2021, respectively. Over the study period, death rates significantly rose for men (P < 0.001) and remained constant for women (P = 0.444). Examination of genitourinary cancers revealed an upward trend for bladder (P = 0.012) and prostate (P = 0.012) cancers, while a stable trend was observed for kidney (P = 0.070) and testicular (P = 0.139) cancers.

Conclusions
The age-standardized rate and trend of genitourinary cancers are rising. Consequently, this study emphasizes the importance of prevention through screening programs, raising awareness, and utilizing appropriate diagnostic methods.
What’s inside matters: an assessment of the family planning content of digital self-care platforms
Authors: Brittingham Sarah , Mitchell Lauren , Zan Trinity 
Published: July 2024

Abstract
Background
Digital technology has proliferated rapidly in low- and middle-income countries in recent decades. This trend will likely persist as costs decrease, dramatically expanding access to reproductive health and family planning (FP) information. As many digital tools aim to support informed choice among individuals with unmet contraceptive need, it is essential that high-quality information is provided. We set out to assess the accuracy and comprehensiveness of FP content in select user-facing digital self-care platforms.

Methods
We identified 29 digital tools in circulation between 2018–2021 and selected 11 that met our eligibility criteria for analysis. Referencing global guidance documents such as the Family Planning Handbook, Medical Eligibility Criteria for Contraceptive Use, and the Digital Health for Social and Behavior Change High Impact Practice Brief, we developed an original rubric outlining 12 key content areas necessary to support informed, person-centered counseling. We applied this to each tool, enabling assignment of a numerical score that represents content accuracy and comprehensiveness across the 12 key areas.

Results
FP content of digital tools varied greatly in accuracy and comprehensiveness. Of the 12 identified key content areas, 5 were included in all 11 tools, while 6 were addressed inconsistently or not at all. Four content areas were the most accurate and comprehensive: complete list of modern methods, duration of protection, dual method use, and return to fertility. The lowest scoring content areas were side effect management, non-contraceptive benefits, effectiveness, side effects, and instructions for use.

Conclusions
Complete, accurate, and evidence-based FP content is a foundational element of quality digital self-care. Inaccuracies and omissions can impact individual user experiences and decision-making in critical ways. FP content quality should be verified before digital tools are scaled or researched at the programmatic level. From this exercise, we developed a checklist for use in conjunction with global guidance documents to improve future FP content of user-facing digital tools.
Brief communication: The rate of switching from first-line to second-line antiretroviral therapy among people living with HIV in Aden City, Yemen
Authors: Al-Haidary Naif Mohammed , Radman Enas Abobakr 
Published: July 2024

Abstract
Background
Effective management of antiretroviral therapy (ART) is crucial in combating the global HIV pandemic. This study, the first of its kind in Yemen, investigates the rate and determinants of switching from first-line to second-line ART among people living with HIV (PLWH) in Aden City, Yemen.

Methods
A retrospective cohort study was conducted using data from PLWH who started first-line ART at Al-Wahda Hospital from 2007 to May 2022. PLWH in prevention of mother-to-child transmission (PMTCT) programs, those already on second-line ART at enrollment, and those with less than 3 months of follow-up were excluded. Cumulative incidence curves and multivariable proportional hazards models were used to identify factors associated with switching, considering death and loss to follow-up as competing risks. Analyses were carried out using IBM SPSS version 26.

Results
Out of 149 PLWH, 18 (12.1%) switched to second-line ART with a cumulative incidence rate of 1.8 per 100 person-years. Significant factors for switching included being older than 33 years (HR: 1.45, 95% CI: 1.12–1.89), having WHO stage 3 disease (HR: 1.58, 95% CI: 1.21–2.06), and being on a TDF-FTC-EFV-based first-line regimen (HR: 1.35, 95% CI: 1.03–1.77). This switching rate is consistent with rates observed in other resource-limited settings, indicating it is neither exceptionally high nor low compared to similar contexts​.

Conclusions
The study highlights key factors associated with switching to second-line ART in Yemen, emphasizing the need for targeted interventions and continuous monitoring to enhance treatment outcomes. These findings are consistent with regional data from other resource-limited settings.
Analysis of convergence between a unified One Health policy framework and imbalanced research portfolio
Authors: Vors Lisa , Raboisson Didier , Lhermie Guillaume 
Published: July 2024

AbstractThe One Health (OH) approach is collaborative, multisectoral, and transdisciplinary, acknowledging the interdependence among animal, human and environmental health. It has garnered attention within the scientific community, particularly in response to the rising prevalence and global spread of emerging and re-emerging infectious diseases. Common OH issues include zoonotic diseases, antimicrobial resistance (AMR), food and water safety, and the human-animal bond. Among various OH topics, AMR represents a well-described, long-term, complex issue, with a substantial global death toll and large economic costs. Whereas interdisciplinary and transdisciplinary teamwork seems appropriate to address such complex challenges, effects on knowledge production are poorly known. In this study, we investigate how the scientific community mobilizes “One Health.” A comparative bibliometric analysis of OH and AMR research enabled us to assess the level of transdisciplinary research, identify emerging themes, through a co-occurrence network analysis of keywords, and disciplines mobilized, through a co-citation network analysis of scientific journals, in research, as well as level of international collaboration through analysis of co-authorship among countries. We detected a lack of consideration for non-communicable diseases (e.g., obesity, diabetes, cardiovascular diseases) and the well-being of human and animal populations in analysis of themes. Furthermore, although many disciplines are involved in OH and AMR research, little attention was given to social sciences, environmental health, economics, and politics. There was a strong influence of major global economic powers, including the United States and China, in scientific research on OH and AMR, as well as substantial collaboration among European countries. The present results indicated that guidelines are needed to address the mentioned concerns, and specific funds are required for underrepresented countries.
Occupational hazards among healthcare workers in Tanzania: a scoping review
Authors: Lyakurwa Dennis M. , Khalfan Seif S. , Mugisha John , Yao Weiguang 
Published: July 2024

Abstract
Purpose
The implementation of occupational health and safety (OHS) measures among healthcare workers in Tanzania is suboptimal, mainly due to a lack of adequate resources. This study aimed to map the available research and identify research gaps on occupational hazards among healthcare workers in Tanzania.

Methods
A scoping review was conducted by searching relevant articles in MEDLINE, Scopus, Science Direct and Web of Science electronic databases. A total of 29 studies were included, and the data were extracted from these studies.

Results
Healthcare workers in Tanzania are exposed to biological, psychosocial, ergonomics, physical and chemical hazards. The majority of the literature involved biological hazards (71%), and research on other hazards was limited.

Conclusion
OHS need to become a priority public health issue to protect healthcare workers in Tanzania. More research is needed to understand the determinants of this problem in Tanzania.
Potential use of other starch sources in the treatment of glycogen storage disease type Ia – an in vitro study
Authors: Monteiro Vaneisse , Colonetti Karina , Pagno Carlos Henrique , Schmidt Helena OS , Sperb-Ludwig Fernanda , Oliveira Bibiana Mello de , Poloni Soraia , Rios Alessandro O , de Souza Carolina F Moura , Schwartz Ida Vanessa Doederlein 
Published: July 2024

Abstract
Background
Glycogen storage disease type Ia (GSD-Ia) is one of the most common hepatic GSD. Its treatment mainly consists of a diet including a high intake of slow-digestion carbohydrates such as raw cornstarch and the restriction of simple sugars. This enables the maintenance of euglycemia and prevents secondary metabolic disorders. Starch is a glucose polymer formed by amylose and amylopectin, which can be obtained from distinct sources. Although uncooked cornstarch has been successfully used in the treatment of GSD-Ia, it can lead to hyperglycemia and weight gain. in vitro andin vivo tests indicated that sweet manioc starch can be potentially used in the treatment of GSD-Ia.

Results
The moisture analysis revealed a variation from 10.3 to 12.8% in the sweet manioc starch samples, whereas the moisture content of uncooked cornstarch ranged from 7.3 to 11.1%. Quantifiable sugar was detected in 3/5 samples of sweet manioc starch and 1/3 samples of uncooked cornstarch. Notably, this uncooked cornstarch brand is widely employed in GSD-Ia treatment in Brazil. Products B and E had higher values of amylopectin and undetectable levels of sugars. A clinical trial is warranted to compare samples F and G and determine the impact of sugar trace in the same dietary source of starch.

Conclusions
Collectively, the results demonstrated possible therapeutic alternatives for GSD-Ia in addition to traditional uncooked cornstarch.
Astrocyte reactivity is associated with tau tangle load and cortical thinning in Alzheimer’s disease
Authors: Guo Tengfei , Li Anqi , Sun Pan , He Zhengbo , Cai Yue , Lan Guoyu , Liu Lin , Li Jieyin , Yang Jie , Zhu Yalin , Zhao Ruiyue , Chen Xuhui , Shi Dai , Liu Zhen , Wang Qingyong , Xu Linsen , Zhou Liemin , Ran Pengcheng , Wang Xinlu , Sun Kun , Lu Jie , Han Ying 
Published: July 2024

Abstract
Background
It is not fully established whether plasma β-amyloid(Aβ)42/Aβ40 and phosphorylated Tau181 (p-Tau181) can effectively detect Alzheimer’s disease (AD) pathophysiology in older Chinese adults and how these biomarkers correlate with astrocyte reactivity, Aβ plaque deposition, tau tangle aggregation, and neurodegeneration.

Methods
We recruited 470 older adults and analyzed plasma Aβ42/Aβ40, p-Tau181, glial fibrillary acidic protein (GFAP), and neurofilament light (NfL) using the Simoa platform. Among them, 301, 195, and 70 underwent magnetic resonance imaging, Aβ and tau positron emission tomography imaging. The plasma Aβ42/Aβ40 and p-Tau181 thresholds were defined as ≤0.0609 and ≥2.418 based on the receiver operating characteristic curve analysis using the Youden index by comparing Aβ-PET negative cognitively unimpaired individuals and Aβ-PET positive cognitively impaired patients. To evaluate the feasibility of using plasma Aβ42/Aβ40 (A) and p-Tau181 (T) to detect AD and understand how astrocyte reactivity affects this process, we compared plasma GFAP, Aβ plaque, tau tangle, plasma NfL, hippocampal volume, and temporal-metaROI cortical thickness between different plasma A/T profiles and explored their relations with each other using general linear models, including age, sex, APOE-ε4, and diagnosis as covariates.

Results
Plasma A+/T + individuals showed the highest levels of astrocyte reactivity, Aβ plaque, tau tangle, and axonal degeneration, and the lowest hippocampal volume and temporal-metaROI cortical thickness. Lower plasma Aβ42/Aβ40 and higher plasma p-Tau181 were independently and synergistically correlated with higher plasma GFAP and Aβ plaque. Elevated plasma p-Tau181 and GFAP concentrations were directly and interactively associated with more tau tangle formation. Regarding neurodegeneration, higher plasma p-Tau181 and GFAP concentrations strongly correlated with more axonal degeneration, as measured by plasma NfL, and lower plasma Aβ42/Aβ40 and higher plasma p-Tau181 were related to greater hippocampal atrophy. Higher plasma GFAP levels were associated with thinner cortical thickness and significantly interacted with lower plasma Aβ42/Aβ40 and higher plasma p-Tau181 in predicting more temporal-metaROI cortical thinning. Voxel-wise imaging analysis confirmed these findings.

Discussion
This study provides a valuable reference for using plasma biomarkers to detect AD in the Chinese community population and offers novel insights into how astrocyte reactivity contributes to AD progression, highlighting the importance of targeting reactive astrogliosis to prevent AD.
Application of circular statistics in temporal distribution of adult mosquitoes in Qingdao, Shandong Province, China, 2021–2023
Authors: Li Binghui , Fu Qiqi , Huang Yiqing , Sun Qintong , Zhao Chunchun , Ma Xiaofang , Liu Yantao 
Published: July 2024

Abstract
Background
Analyses of the temporal distribution of mosquitoes are presented in statistical charts, but it is difficult to prove in statistics whether differences in peak periods exist among different years or habitats. This study aimed to investigate the application of circular statistics in determining the peak period and a comparison of differences.

Methods
Surveillance of adult mosquitoes was conducted twice a month by light traps in five different habitats from March to November for 3 years (2021–2023) in Qingdao, Shandong Province, China. The Kruskal–Wallis test was performed to determine the differences in mosquito density among different years and habitats. Circular statistics and line charts were employed to determine the peak period and a comparison of differences.

Results
Among a total of 14,834 adult mosquitoes comprising five mosquito species from four genera, Culex pipiens pallens was dominant and accounted for 89.6% of the specimens identified. Aedes albopictus, Armigeres subalbatus, and Anopheles sinensis made up 5.7%, 4.2%, and 0.5%, respectively. Culex tritaeniorhynchus accounted for less than 0.1%. The mean mosquito density (females/trap night) for the trapping period was 10.3 in 2021, 5.6 in 2022, and 3.6 in 2023. Among five habitats, the highest mosquito density was 8.9 in livestock sheds, followed by 6.8 in parks, 5.9 in rural dwellings, 5.5 in urban dwellings, and 5.4 in hospitals. No statistically significant differences were found among different years (H = 1.96, d.f. 2, P = 0.376) and habitats (H = 0.45, d.f. 4, P = 0.978). Overall, the peak period of mosquito activity fell in the months from June to September. The peak period among 3 years differed significantly (F(2,7022) = 119.17, P < 0.01), but there were no statistically significant differences in peak period among different habitats (F(4,7020) = −159.09, P > 0.05).

Conclusion
Circular statistics could be effectively combined with statistical charts to elucidate the peak period of mosquitoes and determine the differences in statistics among different years and habitats. These findings will provide valuable information for mosquito control and public health management.

Graphical Abstract
DNA methylation and whole-genome transcription analysis in CD4+ T cells from systemic lupus erythematosus patients with or without renal damage
Authors: Liu Xiaomin , Zhou Siyu , Huang Mengjie , Zhao Ming , Zhang Weiguang , Liu Qun , Song Kangkang , Wang Xu , Liu Jiaona , OuYang Qing , Dong Zheyi , Yang Ming , Li Zhenzhen , Lin Li , Liu Yi , Yu Yang , Liao Simin , Zhu Jian , Liu Lin , Li Wenge , Jia Linpei , Zhang Aihua , Guo Chaomin , Yang LiuYang , Li Qing gang , Bai Xueyuan , Li Ping , Cai Guangyan , Lu Qianjin , Chen Xiangmei 
Published: July 2024

Abstract
Background
Lupus nephritis (LN) is the most common cause of kidney injury in systemic lupus erythematosus (SLE) patients and is associated with increased mortality. DNA methylation, one of the most important epigenetic modifications, has been reported as a key player in the pathogenesis of SLE. Hence, our article aimed to explore DNA methylation in CD4+ T cells from LNs to identify additional potential biomarkers and pathogenic genes involved in the progression of LN.

Methods
Our study enrolled 46 SLE patients with or without kidney injury and 23 healthy controls from 2019 to 2022. CD4+ T cells were sorted for DNA methylation genotyping and RNA-seq. Through bioinformatics analysis, we identified the significant differentially methylated CpG positions (DMPs) only in the LN group and validated them by Bisulfite PCR. Integration analysis was used to screen for differentially methylated and expressed genes that might be involved in the progression of LN, and the results were analyzed via cell experiments and flow cytometry.

Results
We identified 243 hypomethylated sites and 778 hypermethylated sites only in the LN cohort. Three of these DMPs, cg08332381, cg03297029, and cg16797344, were validated by Bisulfite PCR and could be potential biomarkers for LN. Integrated analysis revealed that the expression of BCL2L14 and IFI27 was regulated by DNA methylation, which was validated by azacytidine (5-aza) treatment. The overexpression of BCL2L14 in CD4+ T cells might induce renal fibrosis and inflammation by regulating the differentiation and function of Tfh cells.

Conclusion
Our study identified novel aberrant DMPs in CD4+ T cells only in LN patients and DNA methylation-regulated genes that could be potential LN biomarkers. BCL2L14 is likely involved in the progression of LN and might be a treatment target.
Hypoxia-preconditioned WJ-MSC spheroid-derived exosomes delivering miR-210 for renal cell restoration in hypoxia-reoxygenation injury
Authors: Toghiani Reyhaneh , Azimian Zavareh Vajihe , Najafi Hanyieh , Mirian Mina , Azarpira Negar , Abolmaali Samira Sadat , Varshosaz Jaleh , Tamaddon Ali Mohammad 
Published: July 2024

Abstract
Background
Recent advancements in mesenchymal stem cell (MSC) technology have paved the way for innovative treatment options for various diseases. These stem cells play a crucial role in tissue regeneration and repair, releasing local anti-inflammatory and healing signals. However, challenges such as homing issues and tumorigenicity have led to exploring MSC-exosomes as a promising alternative. MSC-exosomes have shown therapeutic potential in conditions like renal ischemia-reperfusion injury, but low production yields hinder their clinical use.

Methods
To address this limitation, we examined hypoxic preconditioning of Wharton jelly-derived MSCs (WJ-MSCs) 3D-cultured in spheroids on isolated exosome yields and miR-21 expression. We then evaluated their capacity to load miR-210 into HEK-293 cells and mitigate ROS production, consequently enhancing their survival and migration under hypoxia-reoxygenation conditions.

Results
MiR-210 overexpression was significantly induced by optimized culture and preconditioning conditions, which also improved the production yield of exosomes from grown MSCs. The exosomes enriched with miR-210 demonstrated a protective effect by improving survival, reducing apoptosis and ROS accumulation in damaged renal cells, and ultimately promoting cell migration.

Conclusion
The present study underscores the possibility of employing advanced techniques to maximize the therapeutic attributes of exosomes produced from WJ-MSC spheroid for improved recovery outcomes in ischemia-reperfusion injuries.
Beamforming design for Star-RIS assisted secure wireless communication system under hardware impairments
Authors: Shahcheragh Seyedeh Reyhane , Mohamed-Pour Kamal 
Published: July 2024

AbstractSimultaneous transmission and reflection reconfigurable intelligent surface (Star-RIS) has been recently introduced as one of the hopeful technologies in future communications. In this work, we investigate a beamforming design for Star-RIS-assisted secure wireless communications with two practical protocols, namely energy splitting (ES) and mode switching (MS). We assume that our transceivers are not ideal and have residual hardware impairments that lead to distortion noise. We aim to maximize the total secrecy rate by optimizing the beamforming in BS and Star-RIS under the limitation of transmission power consumption. The proposed non-convex optimization problem is solved via successive convex approximation and alternating optimization.