bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma Nucleocapsid Protein: A Potential Beacon for COVID-19 Severity Prediction and Prognostic Insight in Severe Patients with Comorbidities
Authors: Wang Di , Shen Yutao , Wu Jianbo , Li Yaju , Ma Ke , Jiang Guangjie , Li Xiangyu , Qin Huanhuan , Chen Kun , Wang Meihua , Wu Zhiyuan , Guan Ming 
Published: June 2024

Abstract

Objectives: The COVID-19 pandemic poses ongoing challenges to global public health systems, emphasizing the critical necessity for efficient diagnostic and prognostic markers. This study evaluates the MAGLUMI® SARS-CoV-2 Ag N protein chemiluminescent immunoassay (MAG-CLIA) for its analytical performance and its role in predicting disease severity and prognosis among severe COVID-19 patients with comorbidities.
Methods: Analytical validation of plasma MAG-CLIA SARS-CoV-2 Ag N protein encompassed precision, interference, LoQ and linearity. Plasma N protein concentrations and other biomarkers were measured within 48 hours of admission, tracked until discharge or death. The Mann-Whitney U test explored the association between plasma N protein and COVID-19 severity or prognosis. Longitudinal monitoring of plasma N protein dynamics was conducted in representative patients.
Results: MAG-CLIA demonstrated precise quantification of plasma N protein with a CV below 10% and minimal interference. The LoQ was 0.88 ng/L, with a broad linear range. Plasma N protein showed high diagnostic accuracy for COVID-19, achieving 95.42% sensitivity and 78.32% specificity at 2.388 ng/L. Plasma N protein emerged as a valuable prognostic indicator, correlating with mechanical ventilation need and patient survival. Plasma N protein concentrations ≥ 424.3 ng/L (AUC 0.8102, sensitivity 78.38%, specificity 85.48%) were associated with poor prognosis in severe COVID-19 patients with comorbidities.
Conclusions: MAG-CLIA's SARS-CoV-2 N protein detection in plasma demonstrates both analytical reliability and clinical relevance in our inaugural evaluation. As a promising prognostic biomarker for severe COVID-19 patients, it offers crucial insights into disease severity and progression, emphasizing the significance of early monitoring and intervention, especially for patients with comorbidities.
Transperineal ultrasound measurement of the levator plate angle and anorectal angle is a useful method for evaluating uterine prolapse : a prospective cohort study
Authors: Dai Jiangfeng , Liu Shunlan , Li Jiawen , Zhang Shijie , Xu Wu , Lyu Guorong , He Shaozheng 
Published: June 2024

Abstract

Background
Confirming the patient's cervical position using transperineal ultrasound is not an easy task. This study aimed to employ transperineal ultrasound to measure the levator plate angle (LPA) and anorectal angle (ARA) in patients with varying degrees of uterine prolapse and to explore their efficacy in assessing uterine prolapse.
Methods
This prospective study consecutively enrolled 130 female patients who underwent examinations for lower urinary tract or pelvic floor dysfunction symptoms at the Second Affiliated Hospital of Fujian Medical University from August 2022 to December 2023. Participants were divided into three groups based on the Pelvic Organ Prolapse Quantification (POP-Q) system: POP-Q = 0 (n = 45), POP-Q = 1 (n = 45), and POP-Q ≥ 2 (n = 40). Transperineal ultrasound was used to measure the LPA and ARA at rest (rLPA and rARA) and during maximum Valsalva maneuver (vLPA and vARA). Changes in LPA and ARA from rest to maximal Valsalva (ΔLPA and ΔARA) were calculated. The differences in these ultrasound parameters among the three groups were compared. The area under the curve (AUC) was calculated to assess the diagnostic performance.
Results
With increasing severity of uterine prolapse, vLPA decreased progressively, while vARA and ΔARA initially decreased and then increased (P < 0.05). Compared with the POP-Q stage = 0 group, rLPA was reduced in the POP-Q stage ≥ 2 group, and ΔLPA was reduced in the POP-Q stage = 1 and POP-Q stage ≥ 2 groups (P < 0.05). The optimal cutoff values for diagnosing POP-Q stage ≥ 1 were 27.01° for rLPA, -2.29° for vLPA, and − 26.11° for ΔLPA, with corresponding AUCs of 0.65, 0.86, and 0.75. For diagnosing POP-Q stage ≥ 2, the optimal cutoff values were 24.11° for rLPA, -6.97° for vLPA, and − 34.57° for ΔLPA, with corresponding AUCs of 0.67, 0.80, and 0.69. DeLong's test indicated that vLPA had the highest efficacy in diagnosing both POP-Q stage ≥ 1 and POP-Q stage ≥ 2 (P < 0.05).
Conclusion
Transperineal ultrasound measurement of the LPA and ARA is a simple and effective method for assessing uterine prolapse.
Spatial Deconvolution of Cell Types and Cell States at Scale Utilizing TACIT
Authors: Liu Jinze , Huynh Khoa , Tyc Katarzyna , Matuck Bruno Fernandes , Easter Quinn , Pratapa Aditya , Kumar Nikhil , Pérez Paola , Kulchar Rachel , Pranzatelli Thomas , Souza Deiziane , Weaver Theresa , Qu Xufeng , Junior Luiz Soares , Dolhnokoff Marisa , Kleiner David , Hewitt Stephen , Silva Luiz da , Rocha Vanderson , Warner Blake , Byrd Kevin 
Published: June 2024

Abstract

Identifying cell types and states remains a time-consuming, error-prone challenge for spatial biology. While deep learning is increasingly used, it is difficult to generalize due to variability at the level of cells, neighborhoods, and niches in health and disease. To address this, we developed TACIT, an unsupervised algorithm for cell annotation using predefined signatures that operates without training data. TACIT uses unbiased thresholding to distinguish positive cells from background, focusing on relevant markers to identify ambiguous cells in multiomic assays. Using five datasets (5,000,000-cells; 51-cell types) from three niches (brain, intestine, gland), TACIT outperformed existing unsupervised methods in accuracy and scalability. Integrating TACIT-identified cell types with a novel Shiny app revealed new phenotypes in two inflammatory gland diseases. Finally, using combined spatial transcriptomics and proteomics, we discovered under- and overrepresented immune cell types and states in regions of interest, suggesting multimodality is essential for translating spatial biology to clinical applications.
Cervicovaginal lesions among women attending obstetrics and gynaecology at Ho Teaching Hospital
Authors: Smith-Togobo Cecilia , Ametepe Elvis Seyram Korsi , Danso Prince , Fatau Adam Abdul , Yiadom George Osei , Lopez Magalys Cuba , Mbroh Hinterman , Asiamah Emmanuel Akomanin 
Published: June 2024

Abstract

BACKGROUND
Cervicovaginal lesions are a major health problem globally, with 80% of the cases occurring in developing countries. The lesions develop on the surfaces of the vagina and the cervix, usually with no symptoms, and are most often discovered by a routine Pap test. These lesions are most common in women under age 30 but can develop at any age. These lesions have a significant impact on the morbidity and mortality of women.
AIM
This study sought to determine the prevalence of cervicovaginal lesions among women who attended Obstetrics and Gynecology at Ho Teaching Hospital.
METHODOLOGY
The study was a hospital-based, retrospective study that includes all patients who attended the Obstetrics and Gynaecology clinic at the Ho Teaching Hospital from 2013 to September 2022 and had Pap tests conducted on them. ”Patients’ information such as occupation, clinical history, age, and cytological diagnosis were retrieved. The analyzed data was presented as frequencies and percentages and summarized in figures and tables.
RESULTS
A total of 1244 patients’ data were retrieved. Their ages ranged from 10-77+ years, and the majority were 40-49. More than half of the women were employed in the informal sectors (56.4%), and the least were students (4.4%). 91.19% were NILM (Negative for intraepithelial lesions or malignancy), the majority of the cellular abnormalities were ASCUS (Atypical squamous cells of undetermined significance), while the highest infection recorded was Bacterial vaginosis.
CONCLUSION
The study found a high prevalence of cervical-vaginal infections among women and a strong association between age and cellular abnormalities.
Impact of mobile health technologies on human papillomavirus vaccination uptake among mothers of unvaccinated girls aged 9–14 years in Lagos, Nigeria (mHealth-HPVac): Study protocol of a randomised controlled trial
Authors: OKUNADE Kehinde S. , ADEJIMI Adebola A. , ADEKANYE Temitope V. , ALLSOP Matthew J. , ADELABU Hameed , THOMAS-OGODO Olufemi , ONYEKA Tonia C. , LAWANSON Teniola , AKABA Godwin O. , SALAKO Omolola , ANORLU Rose I. , BEREK Jonathan S. 
Published: June 2024

Abstract

Background: Despite the availability of effective vaccines, human papillomavirus (HPV) vaccine uptake remains low in most resource-limited settings including Nigeria. Mobile health technology (mHealth) may empower patients to control their health, reduce inequalities, and improve the uptake of HPV vaccination.
Aim: The “mHealth-HPVac” study will assess the effects of mHealth using short text messages on the uptake of HPV vaccination among mothers of unvaccinated girls aged 9–14 years and also determine the factors influencing the uptake of HPV vaccination among these mothers.
Methods: This protocol highlights a randomised controlled trial involving women aged 25–65 years who will be enrolled on attendance for routine care at the General Outpatient clinics of Lagos University Teaching Hospital, Lagos, Nigeria between July and December 2024. At baseline, n=224 women will be randomised to either a short text message or usual care (control) arm. The primary outcome is vaccination of the participant’s school-age girl(s) at any time during the 6 months of follow-up. The associations between any two groups of continuous variables will be tested using the independent sample t-test (normal distribution) or the Mann-Whitney U test (skewed data) and that of two groups of categorical variables with Chi-square (X2) or Fisher's exact test where appropriate. Using the multivariable binary logistic regression model, we will examine the effects of all relevant sociodemographic and clinical variables on HPV vaccination uptake among mothers of unvaccinated but vaccine-eligible school-age girls. Statistical significance will be defined as A P<0.05.
Discussion: The mHealth-Cervix study will evaluate the impact of mobile technologies on HPV vaccination uptake among mothers of unvaccinated but vaccine-eligible school-age girls in Lagos, Nigeria as a way of contributing to the reduction in the wide disparities in cervical cancer incidence through primary prevention facilitated using health promotion to improve HPV vaccination uptake.
Registration: PACTR202406727470443 (6th June 2024).
EMG activity of abdominal muscles during isometric V-sit in athletes from diverse sports
Authors: Wu Chia-Fen , Wu Chia-Fang , Yan Wei-Cian , Chiu Chih-Hui , Hu Wen-Pin , Wang Shin-Yuan 
Published: June 2024

Abstract

Background
This study aimed to observe the electromyographic (EMG) activities of rectus abdominis (RA) during the isometric V-sit and symmetry of the bilateral RA.
Methods
Sixteen college students were recruited, with 9 weightlifters and 7 soft tennis players. By using Noraxon EMG system, the EMG signals of the left (RA-L) and right RA (RA-R) were investigated during isometric V-sit at heights of 10 cm, 20 cm, and 30 cm from the ground.
Results
EMG signals of RA-L in soft tennis players were 13.6% higher than those in weightlifters for three phases of V-sit (p < .05). During the 10 cm phase, EMG activities of both RA-L and RA-R were significantly higher in soft tennis players compared to weightlifters (p < .05). Large negative correlations were found between phase and EMG amplitude in weightlifters (RA-L: r = − .552; RA-R: r = − .665, p < .05), while positive correlations existed in soft tennis players (RA-L: r = .373; RA-R: r = .631, p < .05).
Conclusion
The study indicated differences in the impact of isometric V-sit heights on RA activation. The correlation trends between RA signals and phases reflected variations in RA strength and control among different exercise types.
Characterization and Genomic Analyses of dsDNA Vibriophage vB_VpaM_XM1, Representing a New Viral Family
Authors: Wei Zuyun , Li Xuejing , Ai Chunxiang , Dang Hongyue 
Published: June 2024

Abstract

Background
Vibrio parahaemolyticus has been a leading cause of foodborne disease outbreaks and infectious diarrhea cases in coastal areas, antibiotic resistance has increased significantly due to widespread antibiotic abuse, bacteriophages (phages) are viruses that specifically infect bacteria, it is necessary to isolate and characterize new phages to broaden our understanding of the ecology, evolution, and diversity of both phages and their bacterial hosts further.
Results
A novel vibriophage vB_VpaM_XM1 (XM1) was described in the present study. The morphological analysis revealed that phage XM1 had Myoviridae-like morphology, with an oblate icosahedral head and a long contractile tail. The genome size of XM1 is 46,056 bp, with a G + C content of 42.51%, encoding 69 open reading frames (ORFs). Moreover, XM1 showed a narrow host range only lysing Vibrio xuii LMG 21346 (T) JL2919, Vibrio parahaemolyticus 1.1997, and Vibrio parahaemolyticus MCCC 1H00029 among the tested bacteria. One-step growth curves showed that XM1 has a 40-minute latent period and 264 plaque-forming units (PFU)/cell burst size. In addition, XM1 exhibited broad pH, thermal, and salinity stability, as well as strong lytic activity, even at a multiplicity of infection (MOI) of 0.001. Multiple genome comparisons and phylogenetic analyses showed that phage XM1 is grouped in a clade with three other phages, including Vibrio phages Rostov 7, X29, and phi 2, and is distinct from all known viral families that have ratified by the standard genomic analysis of the International Committee on Taxonomy of Viruses (ICTV).
Conclusions
Therefore, the above four phages might represent a new viral family, tentatively named Weiviridae. The broad physiological adaptability of phage XM1 and its high lytic activity and host specificity indicated that this novel phage is a good candidate for being used as a therapeutic bioagent against infections caused by certain Vibrio parahaemolyticus strains.
Correlation Study Between Occupational Hazardous Factor Exposome and Bone Mineral Density Abnormality in Steelworkers
Authors: Zhang Haoruo , Zheng Ziwei , Wang Nan , Li Zheng , Zhang Shangmingzhu , Su Yu , Wu Jianhui 
Published: June 2024

Abstract

Objectives
To investigate associations among occupational hazardous factor exposure groups and bone mineral density (BMD) abnormalities in steelworkers, by analyzing correlation between various occupational hazardous factors (night-shift work, high temperature, dust and noise) and BMD abnormalities.
Methods
A total of 6816 participants were recruited for this study. Night-shift work, high temperature, dust and noise was taken as occupational hazardous factors, analyzed as a unique factor by each and as an exposome together. Health risk factor score and partial regression coefficient were used to establish a single risk score exposure group model and a hybrid risk score exposure group model.
Results
Detection rate of abnormal BMD in steelworkers was 27.6%, of which 28.0% in males and 23.3% in females. Single risk score model showed, compared with the control group (score <0.440), steelworkers with scores of 0.440~, 0.778~, and ≥1.078 had a abnormal BMD risk of 1.252 (1.034~1.516) folds, 1.534 (1.253~1.878) times, and 2.234 (1.808~2.759) times. Combined risk score model showed that, compared with the reference group(score<0.366), steelworkers with a score in 0.366~, 0.621~, and ≥0.872 were 1.167 (0.969~1.405) times, 1.470 (1.205~1.794) times, and 2.136 (1.738~2.626) times, respectively.
Conclusions
1 Night-shift work, high temperature, dust, and noise may contribute to BMD abnormality in steelworkers. 2 The higher the corresponding risk score of the occupational hazardous factor exposome, the higher the risk of BMD abnormality in steelworkers. When workers are exposed to multiple occupational hazards at the same time, constructing an exposome could reveal the relationship between occupational hazardous factors and BMD abnormality in steelworkers more accurately.
Synthetic Bacterial Consortia Transplantation Attenuates Vaginal Inflammation and Modulates the Immune Response in a Mouse Model of Gardnerella vaginalis-Induced Bacterial Vaginosis
Authors: Liu Ying , He Liang , Hu Yan , Liao Xingya , Wang Hongyan , Yang Linlin 
Published: June 2024

Abstract

Bacterial vaginosis (BV) disrupts the vaginal microbiota, leading to health risks. Antibiotics often fail to prevent recurrence. This study examines synthetic bacterial consortia transplantation (SBCT) as a safer, more controlled treatment alternative to restore healthy vaginal flora and immune response, showing promise against BV. This study aimed to evaluate the efficacy of SBCT and compare it with VMT in a mouse model of Gardnerella vaginalis-induced BV. A murine model of G. vaginalis-induced BV was established, and mice were treated with SBCT, VMT, or saline. Histopathological changes, inflammatory cytokine levels, pro-inflammatory biomarker expression, helper T cell transcription factor expression, and vaginal microbiota composition were assessed. SBCT and VMT effectively suppressed G. vaginalis growth, reduced inflammation, and restored vaginal microbiota diversity. Both treatments attenuated epithelial damage, downregulated pro-inflammatory cytokines (IL-1β and IL-8), and upregulated the anti-inflammatory cytokine IL-10. SBCT and VMT also inhibited NF-κB activation, suppressed IL-17 expression, and enhanced Foxp3 expression in vaginal tissues. SBCT is a promising therapeutic approach for treating BV, as it effectively modulates the immune response and restores vaginal microbiota diversity in a mouse model of G. vaginalis-induced BV.
Decorated active-intelligent film using urea and fish gelatin quantum dots for improving and detecting the quality of chilled trout fillets
Authors: Zarei Tara , Rezaei Masoud , Bahramifar Nader , Heidari Mahshid Gharib 
Published: June 2024

Abstract

In this study, an active-intelligent film was created by adding urea and fish gelatin quantum dots along with the mucilage, aiming at improving and monitoring the microbial and chemical quality of trout fillets during chilled storage. Fish gelatin and urea quantum dots were successfully synthesized using microwave and low-heat methods. Enriching the alginate film to quantum dots and mucilage resulted in superior mechanical characteristic including thickness (0.0061 ± 0.072 mm), tensile strength (62.8 ± 1.41%), and elongation (35 ± 1.04%) while decreasing some physical properties like solubility (73 ± 5.14%) and water vapor permeability (1.1 ± 0.49 g/m− 2 s− 1). Treatments containing quantum dots indicated inferior transparency and an excellent ability in blocking UV light. The FTIR functional groups proved the presence of film compounds. An uneven surface and weaker crystallinity structure were observed in treatments containing mucilage, recorded by FESEM and XRD. Adding gelatin quantum dots and mucilage led to the lowest level of total (8.4 ± 1 CFU/g− 1), psychrophilic (5.4 ± 1 CFU/g− 1), and H2S-producing (7 ± 1 CFU/g− 1) bacteria after 16 days. Additionally, the lowest level of pH (7.7 ± 0.09), TVB-N (76.6 ± 0.47%), PV (7.48 ± 0.68%), TBA (0.94 ± 0.06), and FFA (1.13 ± 0.004%) were observed in gelatin quantum dots and mucilage contained treatment. After 8 days, the fluorescence intensity of films enhanced due to the increasing level of bacteria. The changes in microbial and chemical indexes were perfectly detected by increasing the fluorescence and color changes of produced films. The active-intelligent films are introduced as a novel potential packaging for prolonging the shelf life along with monitoring the freshness of food.
Experimental and Theoretical Study of Gravity-Driven Falling Plates
Authors: Saenz Leonardo , Tian Ruijun , Jiao Jianqiang , Shu Fangjun 
Published: June 2024

Abstract

This research investigates the fluid-structure interaction phenomenon of gravity-driven falling rigid plates through a combination of experimental and theoretical approaches. Plates of varying dimensions and densities are systematically examined to explore the influence of non-dimensional parameters, including the Reynolds number (Re) and dimensionless moment of inertia (I*), on the falling patterns. High-speed photography is employed to extract plate trajectories and posterior kinematics calculations. In the range of relatively high Reynolds numbers (Re > 800), our study identifies three distinct falling modes: periodic fluttering, periodic tumbling, and marginal chaotic motion. The falling trajectories of the plates are analyzed and compared with their corresponding kinematic behaviors. By integrating theoretical analyses with experimental findings, we develop a semi-analytical model capable of calculating the real-time hydrodynamic forces and moments acting on falling plates. This model facilitates the prediction of falling trajectories for quasi-2-dimensional plates with arbitrary material and dimension combinations. Comparisons between model predictions and experimental results demonstrate good agreement in the fluttering and tumbling modes.
Hot Carrier Localization Affected by A Cations in Hybrid Perovskite
Authors: Zhang Zeyu , Yue Xingyu , Ouyang Yunfei , Zheng Yujie , Sun Kuan , Hu Zhiping , Wang Chunwei , Zu Xinzhi , Liu Zhengzheng , Leng Yuxin , Yin Qinxue , Du Juan 
Published: June 2024

Abstract

Although FAPbI3-based optoelectronic devices have achieved excellent properties, the role of organic cations FA and MA in the performance of organic-inorganic perovskites remains contentious. These cations typically affect the phase stabilization, cubic crystallization, and surface functionalization process, while were traditionally believed to hardly directly affect the optoelectronic properties of organic-inorganic lead halide perovskites (OLHPs). In this study, transient photo-drag current, arising from momentum transfer during light-matter interaction, is employed to reveal the hot carrier transfer dynamics of the OLHPs. The photo-drag current could be reversed by the substitution of the MA cation, which indicates the hot carriers in OLHPs are strongly localized by the MA cation dipole. Additionally, the correlation between the hot carrier photoconductivity and the electronic structure in different A-cation samples revealed that the bulk photovoltaic current could also affected by the A-cation in HIOPs. Our work not only demonstrates the photon drag current in perovskite with an excess photon energy pump but also provides a method to study light-carrier-phonon interactions of the hot carriers in OLHPs.