bluuebunny/crossref_metadata_2025_split huggingface dataset:
The protein structure determines the sensitizing capacity of Brazil nut 2S albumin (Ber e1) in a rat food allergy model
Authors: Van Bilsen Jolanda HM , Knippels Léon MJ , Penninks André H , Nieuwenhuizen Willem F , De Jongh Harmen HJ , Koppelman Stef J 
Published: November 2013

AbstractIt is not exactly known why certain food proteins are more likely to sensitize. One of the characteristics of most food allergens is that they are stable to the acidic and proteolytic conditions in the digestive tract. This property is thought to be a risk factor in allergic sensitization. The purpose of the present study was to investigate the contribution of the protein structure of 2S albumin (Ber e1), a major allergen from Brazil nut, on the sensitizing capacity in vivo using an oral Brown Norway rat food allergy model. Disulphide bridges of 2S albumin were reduced and alkylated resulting in loss of protein structure and an increased pepsin digestibility in vitro. Both native 2S albumin and reduced/alkylated 2S albumin were administered by daily gavage dosing (0.1 and 1 mg) to Brown Norway rats for 42 days. Intraperitoneal administration was used as a positive control. Sera were analysed by ELISA and passive cutaneous anaphylaxis. Oral exposure to native or reduced/alkylated 2S albumin resulted in specific IgG1 and IgG2a responses whereas only native 2S albumin induced specific IgE in this model, which was confirmed by passive cutaneous anaphylaxis. This study has shown that the disruption of the protein structure of Brazil nut 2S albumin decreased the sensitizing potential in a Brown Norway rat food allergy model, whereas the immunogenicity of 2S albumin remained preserved. This observation may open possibilities for developing immunotherapy for Brazil nut allergy.
Graphical modeling and query language for hospitals
Authors: Barzdins Janis , Barzdins Juris , Rencis Edgars , Sostaks Agris 
Published: November 2013

Abstract

Background
So far there has been little evidence that implementation of the health information technologies (HIT) is leading to health care cost savings. One of the reasons for this lack of impact by the HIT likely lies in the complexity of the business process ownership in the hospitals. The goal of our research is to develop a business model-based method for hospital use which would allow doctors to retrieve directly the ad-hoc information from various hospital databases.

Methods
We have developed a special domain-specific process modelling language called the MedMod. Formally, we define the MedMod language as a profile on UML Class diagrams, but we also demonstrate it on examples, where we explain the semantics of all its elements informally. Moreover, we have developed the Process Query Language (PQL) that is based on MedMod process definition language. The purpose of PQL is to allow a doctor querying (filtering) runtime data of hospital’s processes described using MedMod.

Results
The MedMod language tries to overcome deficiencies in existing process modeling languages, allowing to specify the loosely-defined sequence of the steps to be performed in the clinical process.
The main advantages of PQL are in two main areas – usability and efficiency. They are: 1) the view on data through “glasses” of familiar process, 2) the simple and easy-to-perceive means of setting filtering conditions require no more expertise than using spreadsheet applications, 3) the dynamic response to each step in construction of the complete query that shortens the learning curve greatly and reduces the error rate, and 4) the selected means of filtering and data retrieving allows to execute queries in O(n) time regarding the size of the dataset.

Conclusions
We are about to continue developing this project with three further steps. First, we are planning to develop user-friendly graphical editors for the MedMod process modeling and query languages. The second step is to do evaluation of usability the proposed language and tool involving the physicians from several hospitals in Latvia and working with real data from these hospitals. Our third step is to develop an efficient implementation of the query language.
Subcutaneous fatty acid composition of steers finished as weanlings or yearlings with and without growth promotants
Authors: Mapiye Cletos , Turner Tyler D , Basarab John A , Baron Vern S , Aalhus Jennifer L , Dugan Michael ER 
Published: November 2013

Abstract

Background
The current study evaluated the subcutaneous fatty acid (FA) composition of calf- and yearling-fed steers with or without growth promoting implants. Crossbred steers (n = 112; 267 ± 5.0 kg) of the same contemporary group were allocated to one of four production system and implant strategy based treatments in a completely randomized design with a 2 × 2 factorial arrangement of treatments.

Results
There were no interactions (P > 0.05) between production systems and growth promoting implants for the total and individual subcutaneous FA. Yearling as opposed to calf finishing reduced (P < 0.05) subcutaneous proportions of C20:3n-6, trans (t) 12-18:1, C14:0, several minor cis-monounsaturated FA (c-MUFA; c 9-14:1, c 11-16:1, c 11-18:1, c 12-18:1, c 13-18:1, c 9-20:1 and c 11-20:1), and increased (P < 0 .05) subcutaneous proportions of t 11c 15-18:2, total and individual branched-chain FA. Subcutaneous fat from steers implanted with growth promotants had higher (P < 0.05) proportions of total polyunsaturated FA (PUFA), total n-6 PUFA, C18:2n-6 and individual t- 18:1 isomers (t 6 to t 10) compared to non-implanted steers.

Conclusions
Overall, current findings show that production systems and growth promotants led to only minor differences in subcutaneous FA composition of beef steers.
In vitro methods for evaluating therapeutic ultrasound exposures: present-day models and future innovations
Authors: Alassaf Ahmad , Aleid Adham , Frenkel Victor 
Published: November 2013

Abstract
Although preclinical experiments are ultimately required to evaluate new therapeutic ultrasound exposures and devices prior to clinical trials, in vitro experiments can play an important role in the developmental process. A variety of in vitro methods have been developed, where each of these has demonstrated their utility for various test purposes. These include inert tissue-mimicking phantoms, which can incorporate thermocouples or cells and ex vivo tissue. Cell-based methods have also been used, both in monolayer and suspension. More biologically relevant platforms have also shown utility, such as blood clots and collagen gels. Each of these methods possesses characteristics that are well suited for various well-defined investigative goals. None, however, incorporate all the properties of real tissues, which include a 3D environment and live cells that may be maintained long-term post-treatment. This review is intended to provide an overview of the existing application-specific in vitro methods available to therapeutic ultrasound investigators, highlighting their advantages and limitations. Additional reporting is presented on the exciting and emerging field of 3D biological scaffolds, employing methods and materials adapted from tissue engineering. This type of platform holds much promise for achieving more representative conditions of those found in vivo, especially important for the newest sphere of therapeutic applications, based on molecular changes that may be generated in response to non-destructive exposures.
Interactions between Quantum Mixing and the Environmental Dynamics Controlling Ultrafast Photoinduced Electron Transfer and Its Temperature Dependence
Authors: Ishizaki Akihito 
Published: November 2013

Abstract
Primary charge separation in the photosystem II reaction center might take place on subpicosecond time scales. Typically, the time scales of environmental dynamics, such as protein reorganization, also occur in subpicosecond. To explore the potential causes for the subpicosecond charge separation, we theoretically investigate the interactions between environmental dynamics and quantum mixing that affect ultrafast photoinduced electron transfer. The time scales of environmental dynamics change the nature and temperature dependence of reactions to enable subpicosecond reactions.
Acceleration of Fe2O3 Reduction Kinetics by Wet Methane with Calcium Titanate as Support
Authors: Isogai Syunsuke , Kosaka Fumihiko , Takimoto Isao , Hatano Hiroyuki , Oshima Yoshito , Otomo Junichiro 
Published: November 2013

Abstract
To develop new chemical-energy conversion and storage systems using metal oxides, the kinetics of iron oxide (Fe2O3) reduction by humidified methane using an oxide ion conductor, CaTi1−xFexO3−δ (CTFO), as a support was analyzed. Significant improvements in Fe2O3 reduction rate and lattice oxygen utilization were observed using CTFO, which may be induced by rapid ion transport at the interface between Fe2O3 and CTFO.
Behavioral Modeling of SNFS for Synthesizing Multi-Scroll Chaotic Attractors
Authors: Sánchez-López C. , Fernández F. V. , Carbajal-Gómez V. H. , Tlelo-Cuautle E. , Mendoza-López J. 
Published: November 2013

Abstract
Existing models of continuous nonlinear functions used for generating multi-scroll chaotic attractors are based on a piece-wise linear (PWL) approach. These models, although relatively easy to build, do not include any information related to the performance parameters of active devices, in the context of a possible physical implementation. This is a serious drawback, since the use of a PWL model introduces a level of inaccuracy into a numerical analysis which is more evident when numerical and experimental results are compared. This paper proposes a methodology to generate the behavioral model of continuous nonlinear functions, but unlike PWL approaches, real physical active device parameters are herein taken into account. In particular, we generate the behavioral model of a nonlinear function called saturated nonlinear function series (SNFS), but in general, the proposed approach can be used to generate the behavioral model of other continuous nonlinear functions. Our results indicate that the proposed approach yields a more realistic and accurate behavioral model than PWL models. As a consequence, not only the generation of chaotic attractors is more precise, but the metrics used to measure the complexity of a chaotic system can also be better predicted. Numerical and electrical simulation results at both domains, phase and time, illustrate the benefits of the new proposed model.
Peroxisome proliferator-activated receptor-gamma (PPARγ) is down regulated in trophoblast cells of gestational diabetes mellitus (GDM) and in trophoblast tumour cells BeWo in vitro after stimulation with PPARγ agonists
Authors: Knabl Julia , Hüttenbrenner Rebecca , Hutter Stefan , Günthner-Biller Maria , Vrekoussis Thomas , Karl Katrin , Friese Klaus , Kainer Franz , Jeschke Udo 
Published: November 2013

Abstract

Aims: Peroxisome proliferator-activated receptor-gamma (PPARγ) plays an important role in insulin metabolism, trophoblast differentiation and anti-inflammatory circuits. The aim of this study was to investigate the expression of PPARγ in the placenta of patients with gestational diabetes mellitus (GDM) and the regulation of PPARγ by its agonists in trophoblast tumour cells BeWo in vitro.

Methods: PPARγ expression in a total of 80 placentas (40 GDM/40 controls) was analysed by immunohistochemistry using the semi-quantitative immunoreactive score. Furthermore, a quantitative reverse transcription-polymerase chain reaction (PCR) was performed to determine the PPARγ mRNA-expression in both groups. We used a fused and a non-fused BeWo cell culture model for the stimulation with arachidonic acid and 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2). Afterwards PPARγ mRNA-expression was analysed by quantitative real-time PCR (RT-PCR) (TaqMan).

Results: Using immunohistochemistry we identified a decreased expression of PPARγ in the syncytiotrophoblast and the extravillous trophoblast of GDM placentas compared to normal controls. Furthermore, PPARγ mRNA-expression was reduced in GDM placentas. Stimulation of BeWo cells with arachidonic acid and 15d-PGJ2 caused a downregulation of PPARγ expression.

Conclusion: As PPARγ is down regulated by arachidonic acid and 15d-PGJ2, the reduced PPARγ expression in GDM placentas may be due to an altered concentration of fatty acid derivates.
Ribosome Biogenesis in the YeastSaccharomyces cerevisiae
Authors: Woolford John L , Baserga Susan J 
Published: November 2013

AbstractRibosomes are highly conserved ribonucleoprotein nanomachines that translate information in the genome to create the proteome in all cells. In yeast these complex particles contain four RNAs (&gt;5400 nucleotides) and 79 different proteins. During the past 25 years, studies in yeast have led the way to understanding how these molecules are assembled into ribosomes in vivo. Assembly begins with transcription of ribosomal RNA in the nucleolus, where the RNA then undergoes complex pathways of folding, coupled with nucleotide modification, removal of spacer sequences, and binding to ribosomal proteins. More than 200 assembly factors and 76 small nucleolar RNAs transiently associate with assembling ribosomes, to enable their accurate and efficient construction. Following export of preribosomes from the nucleus to the cytoplasm, they undergo final stages of maturation before entering the pool of functioning ribosomes. Elaborate mechanisms exist to monitor the formation of correct structural and functional neighborhoods within ribosomes and to destroy preribosomes that fail to assemble properly. Studies of yeast ribosome biogenesis provide useful models for ribosomopathies, diseases in humans that result from failure to properly assemble ribosomes.
Passive obduction and gravity-driven emplacement of large ophiolitic sheets: The New Caledonia ophiolite (SW Pacific) as a case study?
Authors: Lagabrielle Yves , Chauvet Alain , Ulrich Marc , Guillot Stéphane 
Published: November 2013

AbstractThe 300 km long allochthonous sheet of oceanic mantle forming the New Caledonia ophiolite displays three specific characters: 1) the ophiolite pile lacks concordant sheeted dykes and pillow basalt layers; 2) the ophiolite, refered to as the Peridotite nappe, is thrusted over the basaltic formations of the Poya terrane which are classicaly thought to originate from a different oceanic environment; 3) The basal contact of the ultramafic sheet is remarkably flat all along New-Caledonia and the Peridotite nappe has not been thickened during obduction, rather it experienced significant extension. This suggests that the peridotites have not been emplaced by a tectonic force applied to the rear. New petrological and geochemical results obtained from mantle rocks finally show that the Poya terrane may originate from the same oceanic basin as the peridotites. In this article, we consider such possible cogenetic links and we propose a simple model for the obduction of the New Caledonia ophiolite in which the Poya basalts represent the original cover of the Peridotite nappe. We infer that continuous uplift of the subducted units buried beneath the oceanic lithosphere in the northern part of New Caledonia drove passive uplift of the ophiolite and led to erosion and to initiation of sliding of the basaltic layer. During the Priabonian (latest Eocene), products of the erosion of the basaltic layer were deposited together with sediments derived from the Norfolk passive oceanic margin. These sediments are involved as tectonic slices into an accretionary wedge formed in response to plate convergence. The volcaniclastic sedimentation ends up with the emplacement of large slided blocks of basalts and rafted mafic units that progressively filled up the basin. Obduction process ended with the gravity sliding of the oceanic mantle sheet, previously scalped from its mafic cover. This process is contemporaneous with the exhumation of the HP-LT units of Pouebo and Diahot. Gravity sliding was facilitated by the occurrence of a continuous serpentine sole resulting from metasomatic hydratation of mantle rocks, which developed during the uplift of the Norfolk basement and overlying Diahot and Pouébo units. Progressive emersion of the obducted lithosphere allowed subsequent weathering under subaerial, tropical conditions.
West Timor: a key for the eastern Indonesian geodynamic evolution
Authors: Villeneuve Michel , Bellon Hervé , Martini Rossana , Harsolumakso Agus , Cornée Jean-Jacques 
Published: November 2013

Abstract
Timor Island was at time considered as an example of “accretionary prism” linked to the collision between the Australian block and the Banda arc. However, its geological evolution is more complex. Five main superimposed structural units are distinguished in West Timor. The today structure is the result of three main tectonic events that occurred during the Late Oligocene, Late Early Pliocene and Late Pliocene-Early Pleistocene times, respectively. Our field investigations in the 1990 to 2000 decade completed with geochemical analyses and K-Ar datings (Jurassic and Miocene ages) of magmatism allow to precise the geodynamic evolution of Timor that can be summarized as follows: a first block was detached from Gondwana (unit 2) and drifted to the Asiatic margin until the Late Oligocene when it collided with the Asiatic active margin (unit 3). Then, the new block formed by both 2 and 3 units drifted to the south during the Miocene and the Early Pliocene until it collided with the Australian margin (ASM), by the Late Early Pliocene. Then, the Australian and Timor blocks moved together towards the North-North East during the Late Pliocene until they collided with the Banda fore-arc (unit 4). Later on (Pleistocene), Timor island was capped by the “autochthon” (unit 5) and then on (Quaternary?) by the Banda volcanic arc northward thrusted over the South Banda basin. Taking in consideration its close relationships with both the Australian plate and the Eurasian one. Timor may be considered as a key area for building this geodynamical scenario of Indonesia.
Involvement of the vanilloid receptor 1 in the mechanism of analgetic effect of ketorolac
Authors: O. Yadlovskyi , T. Bukhtiarova , L. Bobkova , I. Tatianchenko , I. Monchak 
Published: November 2013

Abstract
The existing ideas about the ketorolac mechanism of analgetic potency are associated with its effect on COX isoenzymes and its significant potency needs to be explained more completely. Its impact on vanilloid receptors 1 (TRPV1) can also be an effective mechanism of analgetic action. We have evaluated the analgetic potential of ketorolac on the basis of its influence on TRPV1. It is known that Tyr511 and Ser512 amino acids are active central components of TRPV1. Thereby the synthesis of the dipeptide Tyr-Ser has been conducted as a model of the TRPV1 active centre. In our model test, using spectrofluorometry, the formation of intermolecular complexes was shown: “ketorolac - Tyr-Ser” and “capsaicin - Tyr-Ser” at stability constants Kr=0.53 • 10-4 L/mol; Kkor= 0.998 and Kr =0.3 • 10-4 L/mol; Kkor=0.998, respectively. Verification of the data was proved in experiments in vitro (the preparation of portal vein) and in vivo (Tail-flick model), with the TRPV1 agonist and antagonist. On portal vein, it was established (from initial level) by Krebs solution that the range of relaxation of smooth muscles of the portal vein (SM) was as follows at the concentration of capsaicin 0.1 μmol/L +30.3%±5.3%, capsazepine 0.5 μmol/L -3.2±2.7% and ketorolac 1.0 μmol/L -60.0±7.0%. In the experiment, at the above mentioned doses of ketorolac on the background of the action of the capsaicin (capsazepine), the range of relaxation of smooth muscles (SM) of the portal vein was -59.3±5.0% and -63.0±6.0% respectively testifying to the fact that the effect of capsaicinoids was neutralised by ketorolac. On the Tail-flick model, an atypical potentiation of the ketorolac antinociception at simultaneous use with the capsaicin was shown. The obtained data allow suggesting that TRPV1 can activate the ketorolac antinociceptive effects.
Automatic Brain Tumor Detection in T2-weighted Magnetic Resonance Images
Authors: Dvořák P. , Kropatsch W.G. , Bartušek K. 
Published: November 2013

Abstract
This work focuses on fully automatic detection of brain tumors. The first aim is to determine, whether the image contains a brain with a tumor, and if it does, localize it. The goal of this work is not the exact segmentation of tumors, but the localization of their approximate position. The test database contains 203 T2-weighted images of which 131 are images of healthy brain and the remaining 72 images contain brain with pathological area. The estimation, whether the image shows an afflicted brain and where a pathological area is, is done by multi resolution symmetry analysis. The first goal was tested by five-fold cross-validation technique with 100 repetitions to avoid the result dependency on sample order. This part of the proposed method reaches the true positive rate of 87.52% and the true negative rate of 93.14% for an afflicted brain detection. The evaluation of the second part of the algorithm was carried out by comparing the estimated location to the true tumor location. The detection of the tumor location reaches the rate of 95.83% of correct anomaly detection and the rate 87.5% of correct tumor location.
Artificial Neural Networks Application in Modal Analysis of Tires
Authors: Koštial P. , Jančíková Z. , Bakošová D. , Valíček J. , Harničárová M. , Špička I. 
Published: November 2013

Abstract
The paper deals with the application of artificial neural networks (ANN) to tires’ own frequency (OF) prediction depending on a tire construction. Experimental data of OF were obtained by electronic speckle pattern interferometry (ESPI). A very good conformity of both experimental and predicted data sets is presented here. The presented ANN method applied to ESPI experimental data can effectively help designers to optimize dimensions of tires from the point of view of their noise.
Aprendizaje, complejidad y desarrollo: agenda curricular para enseñar en los tiempos actuales
Authors: Labarrere Alberto F. 
Published: November 2013

El presente trabajo aborda la cuestión relativa a la formación que demandan los tiempos actuales. En ella se asume que uno de los ejes del currículo lo constituye la preparación para vivir y actuar en un mundo globalizado, donde las condiciones imponen enseñanza y aprendizaje para la incertidumbre, la variabilidad, la ambigüedad y el desequilibrio. Estas dimensiones, como nunca antes, se muestran consustanciales con la vida. Las consideraciones que aparecen a lo largo de sus reflexiones, llevan al autor a proponer cuatro nodos de aprendizaje que debieran figurar en la agenda de las prioridades educacionales.