bluuebunny/crossref_metadata_2025_split huggingface dataset:
First analysis of 10‐year trends in national factor concentrates usage in haemophilia: data from CHARMS, the Canadian Hemophilia Assessment and Resource Management System
Authors: Traore A. N. , Chan A. K. C. , Webert K. E. , Heddle N. , Ritchie B. , St‐Louis J. , Teitel J. , Lillicrap D. , Iorio A. , Walker I. 
Published: June 2014

SummaryThe Canadian Hemophilia Assessment and Resource Management System (CHARMS) tracks factor concentrates (FC) from the sole suppliers, Canadian Blood Services (CBS) and Hema‐Quebec (HQ), to hospitals and to patients’ homes. Patients FC infusion data are entered into CHARMS at Canadian Hemophilia Treatment Centres (HTCs) then exported to the national database (CentrePoint). From 2000 to 2009, 2260 registered haemophilia A or B patients received FVIII (1 009 097 765 IU) and FIX (272 406 859 IU). Over 91% of FVIII and over 84% of FIX was infused at home. Utilization of FVIII progressively increased; this was accounted for by an increase in the number of patients treated (r = 0.97; P < 0.001), there being a linear relationship between the increase in utilization and the increase in number of patients treated (P < 0.001). There was also a correlation with the annual amount used per patient (r = 0.95; P < 0.001). Utilization of FIX did not increase over time. The highest proportional utilization of both FVIII and FIX was for prophylaxis, and this proportion progressively increased being, in year 10 (2009), 77% and 66% for FVIII and FIX respectively. The proportion used for bleeding remained steady; in year 10 that proportion was 14% for FVIII and 26% for FIX, the use per patient for bleeding decreasing. The HTC‐based CHARMS tracking system is essential, in Canada, for analysing indications for infusion, for predicting utilization and planning for future needs.
Characterization of the Aluminas Formed During the Thermal Decomposition of Boehmite by the Rietveld Refinement Method
Authors: Jiménez Jose A. , Padilla Isabel , López‐Delgado Aurora , Fillali Laila , López‐Andrés Sol 
Published: June 2014

Boehmite synthesized through a sol‐gel route from a nonconventional raw material, as an aluminum waste, was calcined at temperatures between 500 and 1500°C. Quantification of crystalline phases was performed by the Rietveld refinement of the XRD patterns. γ‐Al2O3 is formed at 500°C, and up to 1000°C, is the dominant phase. At temperatures ranging from 1000 to 1400°C, it was observed the appearance of a four‐phase region. The complete transformation into α‐Al2O3 lasts until 12 h at 1400°C or at higher temperatures. The presence of an amorphous phase in calcined samples was confined by direct comparison with a standard of pure α‐Al2O3.
Recurrence and progression in patients with non‐muscle invasive bladder cancer: Prognostic models including multicolor fluorescence in situ hybridization molecular grading
Authors: Lodde Michele , Mian Christine , Mayr Roman , Comploj Evi , Trenti Emanuela , Melotti Roberto , Campodonico Fabio , Maffezzini Massimo , Fritsche Hans‐Martin , Pycha Armin 
Published: June 2014

ObjectiveTo test the prognostic value of multicolor fluorescence in situ hybridization analyses of tumor cells in urine for prediction of the recurrence and progression of tumor in patients with intermediate risk non‐muscle invasive bladder cancer.MethodsA total of 168 patients with non‐muscle invasive bladder cancer were included in the study. Fluorescence in situ hybridization was carried out on the bladder wash urine collected before resection. Tumors were classified as low molecular grading if they had a diploid chromosomal pattern or only a loss of p16 or ch3 aneuploidy, and as high molecular grading if they showed aneuploidy of ch7 or 17. Cox regression models assessed the added prognostic value of fluorescence in situ hybridization for primary tumor recurrence or progression, respectively.ResultsMedian follow up was 67 months. A total of 57% of tumors were classified as low molecular grading. The 2‐ and 5‐year recurrence‐free survival was 68% and 49% for low molecular grading, and 47% and 30% for high molecular grading, respectively. The 2‐ and 5‐year progression‐free survival was 95% and 84% for low molecular grading, and 79% and 58% for high molecular grading tumor patients, respectively. Molecular grading (hazard ratio 1.60; P = 0.03) was associated with recurrence, when also accounting for histopathology and a patient's characteristics. Both cancer severity score (hazard ratio 1.51; P < 0.01) and molecular grading (hazard ratio 2.53; P < 0.01) independently and positively predicted progression in multivariable models. The C‐index for predicting recurrence increased from 0.58 to 0.61 when molecular grading fluorescence in situ hybridization was included in the model, and from 0.68 to 0.72 when predicting progression.ConclusionsFluorescence in situ hybridization‐based molecular grading increases the accuracy of a prognostic model, predicting both recurrence and progression in patients with intermediate risk non‐muscle invasive bladder cancer.
piRNA pathway gene expression in the malaria vector mosquito Anopheles stephensi
Authors: Macias V. , Coleman J. , Bonizzoni M. , James A. A. 
Published: June 2014

AbstractThe ability of transposons to mobilize to new places in a genome enables them to introgress rapidly into populations. The piRNA pathway has been characterized recently in the germ line of the fruit fly, Drosophila melanogaster, and is responsible for downregulating transposon mobility. Transposons have been used as tools in mosquitoes to genetically transform a number of species including Anopheles stephensi, a vector of human malaria. These mobile genetic elements also have been proposed as tools to drive antipathogen effector genes into wild mosquito populations to replace pathogen‐susceptible insects with those engineered genetically to be resistant to or unable to transmit a pathogen. The piRNA pathway may affect the performance of such proposed genetic engineering strategies. In the present study, we identify and describe the An. stephensi orthologues of the major genes in the piRNA pathway, Ago3, Aubergine (Aub) and Piwi. Consistent with a role in protection from transposon movement, these three genes are expressed constitutively in the germ‐line cells of ovaries and induced further after a blood meal.
Liver transplantation for the treatment of homozygous familial hypercholesterolaemia in an era of emerging lipid‐lowering therapies
Authors: Page M. M. , Ekinci E. I. , Jones R. M. , Angus P. W. , Gow P. J. , O'Brien R. C. 
Published: June 2014

AbstractHomozygous familial hypercholesterolaemia (FH) causes severe premature coronary artery disease because of very high levels of low density lipoprotein (LDL)‐cholesterol. Standard lipid‐lowering drugs and LDL‐apheresis may not be sufficiently effective. Liver transplantation replaces defective LDL receptors and vastly improves the lipid profile, and we present the first report of an Australian adult to receive this treatment. Emerging drug treatments for FH may be alternatives to LDL‐apheresis and transplantation, but long‐term safety and efficacy data are lacking for all of these options.
Atrial Pacing Site and Atrioventricular Conduction in Patients Paced for Sinus Node Disease
Authors: WATABE TAICHI , ABE HARUHIKO , KOHNO RITSUKO , OGINOSAWA YASUSHI , HAYASHI KATSUHIDE , OHE HISAHARU , TAMURA MASAHITO , TAKEUCHI MASAAKI , OTSUJI YUTAKA 
Published: June 2014

Atrial Pacing Site and AV ConductionBackgroundUnnecessary ventricular pacing in sinus node disease (SND) must be avoided. To test the hypothesis that in SND, with or without 1st degree atrioventricular (AV) block, cumulative percent ventricular pacing (cum%VP) can be limited by low right atrial septal (LRAS) instead of right atrial appendage (RAA) pacing.MethodsWe studied 102 dual‐chamber pacemaker recipients with SND. The PQ interval on 12‐lead electrocardiogram and the atrial paced to ventricular sensed interval (Ap‐Vs) during LRAS and RAA pacing were measured and compared at implantation, 3 months and 1 year of follow‐up. Group 1 included 62 patients with baseline PQ interval <200 milliseconds during LRAS (n = 28) versus RAA (n = 34) pacing. Group 2 included 40 patients with baseline PQ ≥200 milliseconds during LRAS (n = 20) versus RAA (n = 20) pacing. cum%VP were measured at 3 months and 1 year.ResultsThe characteristics and AV conduction properties were similar and the Ap‐Vs interval was significantly shorter in the LRAS than in the RAA pacing group up to 1 year (193 ± 32 milliseconds vs. 220 ± 27 milliseconds in Group 1; P = 0.003, 222 ± 41 milliseconds vs. 281 ± 30 milliseconds in Group 2; P < 0.001). While cumulative percent atrial pacing was consistently similar, cum%VP was significantly smaller during LRAS than RAA pacing (1 ± 1% vs. 8 ± 18% in Group 1; P = 0.03, 7 ± 10% vs. 48 ± 38% in Group 2; P < 0.001). Similar observations were made with or without left atrial (LA) enlargement.ConclusionCompared with RAA, LRAS pacing showed shorter AV interval in SND patients with or without 1st degree AV block and LA enlargement. This beneficial effect persisted through 1‐year follow‐up, and decreased cum%VP significantly.
Ventricular Fibrillation Triggered by PVCs from Papillary Muscles: Clinical Features and Ablation
Authors: SANTORO FRANCESCO , BIASE LUIGI DI , HRANITZKY PATRICK , SANCHEZ JAVIER E. , SANTANGELI PASQUALE , PERINI ALESSANDRO PAOLETTI , BURKHARDT JOHN DAVID , NATALE ANDREA 
Published: June 2014

VF from Papillary MuscleBackgroundAnimal studies showed that papillary muscles can be sources of ventricular fibrillation (VF) in both the left and right ventricle, but this occurrence in humans has been described only in patients with ischemic heart disease.ObjectiveTo investigate the role of papillary muscle premature ventricular contractions (PVCs) as triggers for VF and the safety and feasibility of catheter ablation in these patients.MethodsSix patients (2 male; age, 40 ± 11 years; 5 with a normal structural heart and 1 with nonischemic cardiomyopathy) with history of VF resulting in repetitive implantable cardioverter defibrillator shocks, despite antiarrhythmic drug therapy, and a papillary muscle focus of PVCs triggering VF were included and underwent mapping and ablation of PVCs.ResultsPVCs were observed to trigger VF and localized by mapping the earliest activation point that matched pace mapping of the same area. In 2 patients, PVCs originated from the left ventricle at the posteromedial papillary muscle; in 4 patients, PVCs originated from the right ventricle, at the posterolateral papillary muscle. Elimination of the triggering PVC was obtained in these areas after 19 ± 12 minutes by radiofrequency application. During a follow‐up of 58 ± 11 months using ambulatory monitoring and defibrillator memory interrogation, no patients had recurrence of symptomatic ventricular arrhythmias.ConclusionPapillary muscles from both ventricles represent an anatomic structure potentially involved in the onset of VF, also in normal structural heart. PVCs arising from this area can be successfully eliminated by radiofrequency ablation, resulting in freedom from recurrent VF at long‐term follow‐up.
Quantification of dynamic changes to blood volume and vascular flow in the primate corpus luteum during the menstrual cycle
Authors: Bishop Cecily V. , Molskness Theodore A. , Xu Fuhua , Belcik J. Todd , Lindner Jonathan R. , Slayden Ov D. , Stouffer Richard L. 
Published: June 2014

AbstractBackgroundThe objective of the current study was to determine changes to vascular parameters of nonhuman primate dominant ovarian structures by dynamic contrast‐enhanced ultrasound (DCE‐US).Materials and MethodsDynamic contrast‐enhanced ultrasound with intravenous microbubble infusion was performed on the rhesus macaque ovary bearing the pre‐ovulatory follicle and corpus luteum (CL) sequentially during the natural luteal phase (n = 8) and GnRH antagonist (antide)‐induced luteal regression (n = 6).ResultsChanges in luteal blood volume (BV) and vascular flow (VF) were observed between stages of the luteal phase Luteal BV was highest in early stage CL, before decreasing 2.5‐fold in late stage CL (P < 0.06); in contrast, luteal VF peaked at mid luteal stage (P < 0.01). Two females identified with luteal insufficiency trended toward lower peak BV, compared to typical CLs. Another female was identified with a luteal cyst on the contralateral ovary, and a CL that regressed before P levels declined. After 72 hours of antide exposure, BV was reduced 2.3‐fold (P = 0.03).ConclusionsDCE‐US provides a sensitive, non‐invasive measurement of the dynamics of blood volume and flow in dominant ovarian structures.
New Uses for Rehabilitation Protocol for Oral Sinus Communications in ARONJ Patients
Authors: de Almeida Fernanda Campos Sousa , Moreira Maria Stella , Marcucci Marcelo , Marques Marcia Martins , de Araujo Maria Ercilia , da Silva Dorival Pedroso 
Published: June 2014

AbstractThis report presents a new use for rehabilitation protocol for oral sinus communications in patients with antiresorptive agent‐induced osteonecrosis of the jaw. The treatment plan consisted of constructing an atraumatic complete denture with rounded edges, made with nontoxic resin, to prevent any injury to the mucosa and recurrence of the disease. The patient was followed up for 4 years, without any complications, and was socially reintegrated by resuming the normal life he experienced before tooth loss.
Impact of dental anxiety and fear on dental care use inBrazilian women
Authors: Goettems Marília Leão , Schuch Helena Silveira , Demarco Flávio Fernando , Ardenghi Thiago Machado , Torriani Dione Dias 
Published: June 2014

AbstractObjectivesThis study investigated the relationship between dental anxiety and fear, demographic and socioeconomic characteristics, and dental attendance patterns in a sample of women inBrazil.MethodsA cross‐sectional study of women in healthcare centers was conducted during an immunization campaign in the city ofPelotas in southernBrazil. Interviews were conducted to assess dental anxiety and fear, education level, family income, marital status, and the use of dental services. Data were analyzed byPoisson regression models, with estimation of the prevalence ratio and the rate ratio (RR).ResultsA total of 608 women aged 16‐50 years (mean age 29.3 ± 7.2 years) were included in the study. Dental anxiety and fear scores (according toCorah'sDentalAnxietyScale) ranged from 4 to 20. Of the 608 participants, 59.5 percent displayed low dental fear, 18.1 percent had moderate dental fear, and 22.4 percent displayed high dental fear. A total of 60.2 percent of the women exhibited irregular dental attendance patterns, characterized by never visiting a dentist, or only visiting when experiencing pain. After adjustments, the presence of at least moderate dental anxiety and fear was associated with low education levels (RR1.43; 95 percentCI1.11‐1.84), low family income (RR1.33; 95 percentCI1.06‐1.68), and irregular dental attendance patterns (RR1.83; 95 percentCI1.41‐2.37).ConclusionsIn this sample ofBrazilian women, dental anxiety and fear were strongly associated with socioeconomic characteristics and dental attendance patterns.
The novel globin protein fungoglobin is involved in low oxygen adaptation of Aspergillus fumigatus
Authors: Hillmann Falk , Linde Jörg , Beckmann Nicola , Cyrulies Michael , Strassburger Maria , Heinekamp Thorsten , Haas Hubertus , Guthke Reinhard , Kniemeyer Olaf , Brakhage Axel A. 
Published: June 2014

SummaryThe human pathogenic fungus Aspergillus fumigatus normally lives as a soil saprophyte. Its environment includes poorly oxygenated substrates that also occur during tissue invasive growth of the fungus in the human host. Up to now, few cellular factors have been identified that allow the fungus to efficiently adapt its energy metabolism to hypoxia. Here, we cultivated A. fumigatus in an O2‐controlled fermenter and analysed its responses to O2 limitation on a minute timescale. Transcriptome sequencing revealed several genes displaying a rapid and highly dynamic regulation. One of these genes was analysed in detail and found to encode fungoglobin, a previously uncharacterized member of the sensor globin protein family widely conserved in filamentous fungi. Besides low O2, iron limitation also induced transcription, but regulation was not entirely dependent on the two major transcription factors involved in adaptation to iron starvation and hypoxia, HapX and SrbA respectively. The protein was identified as a functional haemoglobin, as binding of this cofactor was detected for the recombinant protein. Gene deletion in A. fumigatus confirmed that haem‐binding fungoglobins are important for growth in microaerobic environments with O2 levels far lower than in hypoxic human tissue.
Functional mapping of community‐acquired respiratory distress syndrome (CARDS) toxin of Mycoplasma pneumoniae defines regions with ADP‐ribosyltransferase, vacuolating and receptor‐binding activities
Authors: Kannan Thirumalai R. , Krishnan Manickam , Ramasamy Kumaraguruparan , Becker Argentina , Pakhomova Olga N. , Hart P. John , Baseman Joel B. 
Published: June 2014

SummaryCommunity‐acquired respiratory distress syndrome (CARDS) toxin from Mycoplasma pneumoniae is a 591‐amino‐acid virulence factor with ADP‐ribosyltransferase (ADPRT) and vacuolating activities. It is expressed at low levels during in vitro growth and at high levels during colonization of the lung. Exposure of experimental animals to purified recombinant CARDS toxin alone is sufficient to recapitulate the cytopathology and inflammatory responses associated with M. pneumoniae infection in humans and animals. Here, by molecular modelling, serial truncations and site‐directed mutagenesis, we show that the N‐terminal region is essential for ADP‐ribosylating activity. Also, by systematic truncation and limited proteolysis experiments we identified a portion of the C‐terminal region that mediates toxin binding to mammalian cell surfaces and subsequent internalization. In addition, the C‐terminal region alone induces vacuolization in a manner similar to full‐length toxin. Together, these data suggest that CARDS toxin has a unique architecture with functionally separable N‐terminal and C‐terminal domains.
Maize Elongin C interacts with the viral genome‐linked protein, VPg, of Sugarcane mosaic virus and facilitates virus infection
Authors: Zhu Min , Chen Yuting , Ding Xin Shun , Webb Stephen L. , Zhou Tao , Nelson Richard S. , Fan Zaifeng 
Published: June 2014

Summary

The viral genome‐linked protein, VPg, of potyviruses is involved in viral genome replication and translation. To determine host proteins that interact with Sugarcane mosaic virus (SCMV) VPg, a yeast two‐hybrid screen was used and a maize (Zea mays) Elongin C (ZmElc) protein was identified.

ZmELC transcript was observed in all maize organs, but most highly in leaves and pistil extracts, and ZmElc was present in the cytoplasm and nucleus of maize cells in the presence or absence of SCMV. ZmELC expression was increased in maize tissue at 4 and 6 d post SCMV inoculation. When ZmELC was transiently overexpressed in maize protoplasts the accumulation of SCMV RNA was approximately doubled compared with the amount of virus in control protoplasts.

Silencing ZmELC expression using a Brome mosaic virus‐based gene silencing vector (virus‐induced gene silencing) did not influence maize plant growth and development, but did decrease RNA accumulation of two isolates of SCMV and host transcript encoding ZmeIF4E during SCMV infection. Interestingly, Maize chlorotic mottle virus, from outside the Potyviridae, was increased in accumulation after silencing ZmELC expression.

Our results describe both the location of ZmElc expression in maize and a new activity associated with an Elc: support of potyvirus accumulation.
Predictors and Outcomes of Community Agency Use in Abused Mothers
Authors: Gilroy Heidi , Maddoux John , Symes Lene , Fredland Nina , McFarlane Judith 
Published: June 2014

AbstractObjectiveTo identify characteristics of abused mothers that predict use and difficulty of use of community agencies for intimate partner violence (IPV) and to describe the outcomes of community agency use.Design and SampleA prospective study design was used. Three hundred mothers who had experienced physical or sexual IPV and were seeking services for the first time at either a safe shelter for abused women or applying for a protection order.MeasuresData were collected and analyzed by the research team. Predictors were tested using binary logistic regression and outcomes were tested using a series of Mann‐Whitney U nonparametric test of difference on change scores between baseline and 24‐month scores.ResultsNo individual predictors were statistically significant and/or meaningful for use or difficulty using community agencies. There were significant differences in change scores for severity of violence after women used counseling, the police, or legal services. There were also significant change scores for anxiety after women used legal services and social services.ConclusionsAbused women need individualized attention to assure that they can access the services they need to recover from IPV. Evidence‐based practice is needed for community agencies to treat abused women to facilitate safety and health.
Pharmacological Treatment for Common Prostatic Conditions in Dogs – Benign Prostatic Hyperplasia and Prostatitis: an Update
Authors: Niżański W , Levy X , Ochota M , Pasikowska J 
Published: June 2014

ContentsThe two most frequent prostatic diseases in dogs are benign prostatic hyperplasia (BPH) and prostatitis. Prostatitis requires prolonged antibiotic treatment. In acute prostatitis, the blood–prostate barrier is broken, thus facilitating the penetration of antibiotics, whereas in chronic prostatitis, the barrier prevents the penetration of many drugs into the gland. The selection of antibiotic agents is based on the sensitivity test and the drug's ability to penetrate into the gland. Many protocols for the treatment ofBPHare available. In non‐breeding dogs, surgical and optionally pharmacological castration by means of GnRHagonists may be performed. In breeding dogs, drugs retaining fertility are used. Recently, androgen receptor antagonistic treatment with osaterone acetate has been applied. Other drugs used forBPHtreatment include progestagens, oestrogens, antioestrogens and 5α‐reductase inhibitors. Some of these compounds may provoke severe side effects. The efficiency of GnRHantagonists used for the treatment of prostatic diseases, such as neoplasia andBPH, in humans has been recently investigated in dogs. This androgen deprivation therapy (ADT) is devoid of an initial exacerbation of androgen‐dependent symptoms, which is typical for GnRHagonistic treatment. In many cases,BPHand prostatitis must be treated simultaneously as these conditions may develop in combination.