bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construct Validation of the Health Belief Model
Authors: Cummings K. Michael , Jette Alan M. , Rosenstock Irwin M. 
Published: October 2015

A multitrait-multimethod design was employed to assess the construct validity of the Health Belief Model. The data were obtained from a non-representative sample of 85 graduate students at The University of Michigan's School of Public Health. The traits consisted of the respondents' perceptions of: health interest, locus of control, susceptibility to influenza, severity of influenza, benefits provided by a flu shot, and the barriers or costs associated with getting a flu shot. Each trait was measured by three methods: a seven-point Likert scale, a fixed-alternative multiple choice scale, and a vignette. The results indicate that the Health Belief Model variables can be measured with a substantial amount of convergent validity using Likert or multiple choice questionnaire items. With regard to discriminant validity, evidence suggests that subjects' perceptions of barriers and benefits are quite different from their perceptions of susceptibility and severity. Perceptions of susceptibility and severity are substantially but not entirely independent. Perceived benefits and barriers demonstrate a strong negative relationship which suggests the possibility that these two variables represent opposite ends of a single continuum and not separate health beliefs. These preliminary results provide the basis for developing brief health belief scales that may be administered to samples of consumers and providers to assess educational needs. Such needs assessment, in turn, could then be used to tailor messages and programs to meet the particular needs of a client group.
Profiling of somatic mutations in acute myeloid leukemia with FLT3-ITD at diagnosis and relapse
Authors: Garg Manoj , Nagata Yasunobu , Kanojia Deepika , Mayakonda Anand , Yoshida Kenichi , Haridas Keloth Sreya , Zang Zhi Jiang , Okuno Yusuke , Shiraishi Yuichi , Chiba Kenichi , Tanaka Hiroko , Miyano Satoru , Ding Ling-Wen , Alpermann Tamara , Sun Qiao-Yang , Lin De-Chen , Chien Wenwen , Madan Vikas , Liu Li-Zhen , Tan Kar-Tong , Sampath Abhishek , Venkatesan Subhashree , Inokuchi Koiti , Wakita Satoshi , Yamaguchi Hiroki , Chng Wee Joo , Kham Shirley-Kow Yin , Yeoh Allen Eng-Juh , Sanada Masashi , Schiller Joanna , Kreuzer Karl-Anton , Kornblau Steven M. , Kantarjian Hagop M. , Haferlach Torsten , Lill Michael , Kuo Ming-Chung , Shih Lee-Yung , Blau Igor-Wolfgang , Blau Olga , Yang Henry , Ogawa Seishi , Koeffler H. Phillip 
Published: October 2015

Key Points
MLL3 acts as tumor suppressor in FLT3-ITD AML. The existence of DNMT3A mutations in remission samples implies that the DNMT3A mutant clone can survive induction chemotherapy.
The novel mechanism of lenalidomide activity
Authors: Fink Emma C. , Ebert Benjamin L. 
Published: October 2015

Abstract
Lenalidomide acts by a novel drug mechanism—modulation of the substrate specificity of the CRL4CRBN E3 ubiquitin ligase. In multiple myeloma, lenalidomide induces the ubiquitination of IKZF1 and IKZF3 by CRL4CRBN. Subsequent proteasomal degradation of these transcription factors kills multiple myeloma cells. In del(5q) myelodysplastic syndrome, lenalidomide induces the degradation of CK1α, which preferentially affects del(5q) cells because they express this gene at haploinsufficient levels. In the future, modulation of ubiquitin ligase function may enable us to target previously “undruggable” proteins.
The biogeographic origin of a radiation of trees in Madagascar: implications for the assembly of a tropical forest biome
Authors: Federman Sarah , Dornburg Alex , Downie Alexander , Richard Alison F. , Daly Douglas C. , Donoghue Michael J. 
Published: October 2015

AbstractBackgroundMadagascar’s rain forests are characterized by extreme and uneven patterns of species richness and endemicity, the biogeographic and evolutionary origins of which are poorly understood.MethodsHere we use a time-calibrated phylogeny of a dominant group of trees in Madagascar’s eastern rain forests,Canarium, and related Burseraceae (Canarieae), to test biogeographic hypotheses regarding the origin and radiation of the flora of this unique biome.ResultsOur findings strongly support the monophyly of MalagasyCanarium, suggesting that this clade represents a previously undocumented in situ radiation. Contrary to expectations of dispersal from Africa during the Oligocene, concurrent with the formation of Madagascar’s rain forest biome, our analyses support a late Miocene origin for MalagasyCanarium, probably by long distance dispersal from Southeast Asia.DiscussionOur study illustrates the importance of considering long distance dispersal as a viable explanation for clades with pantropical distributions diversifying subsequent to the Oligocene, and it highlights the formation of the Indo-Australian Archipelago and associated fast-moving equatorial surface currents, suggesting an under-appreciated evolutionary link among tropical centers of endemism.ConclusionsWe postulate that the relatively recent establishment and radiation ofCanariumin Madagascar may have been facilitated by the highly stochastic climates associated with these forest ecosystems.
Deeply divergent sympatric mitochondrial lineages of the earthworm Lumbricus rubellus are not reproductively isolated
Authors: Giska Iwona , Sechi Pierfrancesco , Babik Wiesław 
Published: October 2015

AbstractBackgroundThe accurate delimitation of species is essential to numerous areas of biological research. An unbiased assessment of the diversity, including the cryptic diversity, is of particular importance for the below ground fauna, a major component of global biodiversity. On the British Isles, the epigeic earthwormLumbricus rubellus, which is a sentinel species in soil ecotoxicology, consists of two cryptic taxa that are differentiated in both the nuclear and the mitochondrial (mtDNA) genomes. Recently, several deeply divergent mtDNA lineages were detected in mainland Europe, but whether these earthworms also constitute cryptic species remains unclear. This information is important from an evolutionary perspective, but it is also essential for the interpretation and the design of ecotoxicological projects. In this study, we used genome-wide RADseq data to assess the reproductive isolation of the divergent mitochondrial lineages ofL. rubellusthat occur in sympatry in multiple localities in Central Europe.ResultsWe identified five divergent (up to 16 % net p-distance) mitochondrial lineages ofL. rubellusin sympatry. Because the clustering of the RADseq data was according to the population of origin and not the mtDNA lineage, reproductive isolation among the mtDNA lineages was not likely. Although each population contained multiple mtDNA lineages, subdivisions within the populations were not observed for the nuclear genome. The lack of fixed differences and sharing of the overwhelming majority of nuclear polymorphisms between localities, indicated that the populations did not constitute allopatric species. The nucleotide diversity within the populations was high, 0.7–0.8 %.ConclusionsThe deeply divergent mtDNA sympatric lineages ofL. rubellusin Central Europe were not reproductively isolated groups. The earthwormL. rubellus, which is represented by several mtDNA lineages in continental Europe, apparently is a single highly polymorphic species rather than a complex of several cryptic species. This study demonstrated the critical importance of the use of multilocus nuclear data for the unbiased assessment of cryptic diversity and for the delimitation of species in soil invertebrates.
Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol
Authors: Xu Fen , Liu Junli , Deng Jie , Chen Xiaolei , Wang Yuan , Xu Pengchao , Cheng Lin , Fu Yanli , Cheng Fuyi , Yao Yunqi , Zhang Yujing , Huang Meijuan , Yu Dechao , Wei Yuquan , Deng Hongxin 
Published: October 2015

AbstractThe generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity. Upon transplantation into mice with carbon tetrachloride (CCl4)-induced acute fulminant liver failure, iHeps restore the liver function and prolong survival. The work could contribute to the development of alternative strategies to obtain nonhepatic cell-derived mature hepatocytes with potential for biomedical and pharmaceutical applications.
A rapid in vivo screen for pancreatic ductal adenocarcinoma therapeutics
Authors: Ocal Ozhan , Pashkov Victor , Kollipara Rahul K. , Zolghadri Yalda , Cruz Victoria H. , Hale Michael A. , Heath Blake R. , Artyukhin Alex B. , Christie Alana L. , Tsoulfas Pantelis , Lorens James B. , Swift Galvin H. , Brekken Rolf A. , Wilkie Thomas M. 
Published: October 2015

ABSTRACT
Pancreatic ductal adenocarcinoma (PDA) is the fourth leading cause of cancer-related deaths in the United States, and is projected to be second by 2025. It has the worst survival rate among all major cancers. Two pressing needs for extending life expectancy of affected individuals are the development of new approaches to identify improved therapeutics, addressed herein, and the identification of early markers. PDA advances through a complex series of intercellular and physiological interactions that drive cancer progression in response to organ stress, organ failure, malnutrition, and infiltrating immune and stromal cells. Candidate drugs identified in organ culture or cell-based screens must be validated in preclinical models such as KIC (p48Cre;LSL-KrasG12D;Cdkn2af/f) mice, a genetically engineered model of PDA in which large aggressive tumors develop by 4 weeks of age. We report a rapid, systematic and robust in vivo screen for effective drug combinations to treat Kras-dependent PDA. Kras mutations occur early in tumor progression in over 90% of human PDA cases. Protein kinase and G-protein coupled receptor (GPCR) signaling activates Kras. Regulators of G-protein signaling (RGS) proteins are coincidence detectors that can be induced by multiple inputs to feedback-regulate GPCR signaling. We crossed Rgs16::GFP bacterial artificial chromosome (BAC) transgenic mice with KIC mice and show that the Rgs16::GFP transgene is a KrasG12D-dependent marker of all stages of PDA, and increases proportionally to tumor burden in KIC mice. RNA sequencing (RNA-Seq) analysis of cultured primary PDA cells reveals characteristics of embryonic progenitors of pancreatic ducts and endocrine cells, and extraordinarily high expression of the receptor tyrosine kinase Axl, an emerging cancer drug target. In proof-of-principle drug screens, we find that weanling KIC mice with PDA treated for 2 weeks with gemcitabine (with or without Abraxane) plus inhibitors of Axl signaling (warfarin and BGB324) have fewer tumor initiation sites and reduced tumor size compared with the standard-of-care treatment. Rgs16::GFP is therefore an in vivo reporter of PDA progression and sensitivity to new chemotherapeutic drug regimens such as Axl-targeted agents. This screening strategy can potentially be applied to identify improved therapeutics for other cancers.
From drug response profiling to target addiction scoring in cancer cell models
Authors: Yadav Bhagwan , Gopalacharyulu Peddinti , Pemovska Tea , Khan Suleiman A. , Szwajda Agnieszka , Tang Jing , Wennerberg Krister , Aittokallio Tero 
Published: October 2015

ABSTRACT
Deconvoluting the molecular target signals behind observed drug response phenotypes is an important part of phenotype-based drug discovery and repurposing efforts. We demonstrate here how our network-based deconvolution approach, named target addiction score (TAS), provides insights into the functional importance of druggable protein targets in cell-based drug sensitivity testing experiments. Using cancer cell line profiling data sets, we constructed a functional classification across 107 cancer cell models, based on their common and unique target addiction signatures. The pan-cancer addiction correlations could not be explained by the tissue of origin, and only correlated in part with molecular and genomic signatures of the heterogeneous cancer cells. The TAS-based cancer cell classification was also shown to be robust to drug response data resampling, as well as predictive of the transcriptomic patterns in an independent set of cancer cells that shared similar addiction signatures with the 107 cancers. The critical protein targets identified by the integrated approach were also shown to have clinically relevant mutation frequencies in patients with various cancer subtypes, including not only well-established pan-cancer genes, such as PTEN tumor suppressor, but also a number of targets that are less frequently mutated in specific cancer types, including ABL1 oncoprotein in acute myeloid leukemia. An application to leukemia patient primary cell models demonstrated how the target deconvolution approach offers functional insights into patient-specific addiction patterns, such as those indicative of their receptor-type tyrosine-protein kinase FLT3 internal tandem duplication (FLT3-ITD) status and co-addiction partners, which may lead to clinically actionable, personalized drug treatment developments. To promote its application to the future drug testing studies, we have made available an open-source implementation of the TAS calculation in the form of a stand-alone R package.
Improving translational studies: lessons from rare neuromuscular diseases
Authors: Wells Dominic 
Published: October 2015

ABSTRACT
Animal models play a key role in the development of novel treatments for human disease. This is particularly true for rare diseases – defined as disorders that affect less than 1 in 2000 people in the human population – for which, very often, there are no effective methods of treatment. Pharmaceutical companies are increasingly focussing on the development of therapies for the more than 7000 rare diseases. Because the majority of these are the result of single gene disorders, the exceptional ability to manipulate the mouse genome means that many such studies will take place in the laboratory mouse. But how good are the mouse models and how useful are they in assessing the potential for translational medicine? In this Editorial, I will discuss current difficulties in translational research as well as examples of good laboratory practice and guidelines that are being implemented to improve the translational potential of animal studies in the field of neuromuscular rare diseases. This could represent a potentially useful approach for adoption by other disease fields to achieve a greater success rate in translational studies.
Chekhov’s landscapes: Building story structure with descriptions of the natural world
Authors: Searfoorce Geeda 
Published: October 2015

Abstract
While there are myriad lenses through which we can examine Anton Chekhov’s mastery of the short-story form, one of the clearest is his use of the natural world. The writer often uses descriptions to build story structure and transmogrify the protagonist’s experience, all the while highlighting a theme pervasive throughout his work – that of humankind’s damaging effect on the natural world and the implications of reciprocal damage. A close look at two of Chekhov’s later stories – ‘Peasants’ and ‘In the Ravine’ – reveals his ability to paint, in simple, evocative language, landscapes that do far more than serve as mere backdrops.
Reviews
Authors: Järviö Päivi , Bell Gelsey , Kapadocha Christina , Melillo John , Nickell Chris 
Published: October 2015

Abstract
Dramatic Theories of Voice in the Twentieth Century, Andrew M. Kimbrough (2011) Amherst, NY: Cambria Press, 334 pp. ISBN: 9781604977301, h/bk, $114.99
The Sound Studies Reader, Jonathan Sterne (ed.) (2012) London: Routledge, x + 566 pp. ISBN: 9780415771306, h/bk, $135 ISBN: 9780415771313, p/bk, $49.95
Voice into Acting: Integrating Voice and the Stanislavs ki Approach, Christina Gutekunst and John Gillett (2014) London: Bloomsbury, 384 pp. ISBN: 9781408184509, p/bk, $26.99
Lexicon of the Mouth: Poetics and Politics of Voice and the Oral Imaginary, Brandon LaB elle (2014) London: Bloomsbury, 232 pp. ISBN: 9781623560263, h/bk, £50.00 ISBN: 9781623561888, p/bk, £19.99
Voice Studies Now Conference, University of California, Los Angeles, 29–30 January 2015 https://voicestudiesconference.wordpress.com/
Competencia emocional y rendimiento académico en el alumnado universitario
Authors: Suberviola Ovejas Iratxe 
Published: October 2015

Este estudio presenta la finalidad de evidenciar los beneficios de la inclusión de la Educación Emocional en las diferentes etapas educativas, concretamente como fomento del rendimiento académico del alumnado universitario. Para ello, se realiza un análisis en el que se correlacionan la variablepuntuaciones obtenidas en el TMMS-24 con la variablecalificaciones académicas en una muestra de alumnos y alumnas de 1º curso de grado de la Universidad de La Rioja. Los instrumentos utilizados son: el TMMS-24; El examen teórico; Rúbrica para las prácticas.Se lleva a cabo un análisis cuantitativo correlacional, entre la variable puntuaciones obtenidas en el TMMS-24 y la variablecalificaciones académicas, a tres niveles; 1) de toda la muestra y dividida por los grupos establecidos según las puntuaciones obtenidas en el TMMS-24 en cada una de las sub-escalas; 2) la comparación de los promedios en las calificaciones teórica y práctica, según los cuartiles establecidos con las puntuaciones obtenidas en el TMMS-24; 3) el análisis correlacional de las variables, aplicando la prueba de Pearson emparejando, tanto la puntuación total como la puntuación de las tres sub-escalas del TMMS-24, con las calificaciones obtenidas en la parte práctica y la teórica.
Investigación en comunicación municipal: estudios y aportaciones académicas
Authors: Campillo Alhama Conchi 
Published: October 2015

Los Ayuntamientos se dirigen a la ciudadanía a través de procesos comunicativos y relacionales para transmitir todo tipo de mensajes sobre la gestión pública; tales procesos se justifican desde la premisa fundamental de que el ciudadano debe estar informado de todas aquellas actuaciones e iniciativas administrativas que pueden incidir en su condición de administrado. La comunicación pública municipal constituye, en este sentido, una poderosa herramienta en manos de los equipos de gobierno que permite explicar, justificar y, en consecuencia, legitimar las decisiones políticas asumidas en cada periodo legislativo. En este trabajo se realiza una revisión de las principales aportaciones y contribuciones académicas sobre la comunicación de proximidad, ya que creemos que la investigación académica sobre los procesos de comunicación pública desarrollados en este ámbito puede contribuir, de forma notable, a aumentar la profesionalización de los gabinetes de comunicación en las administraciones locales. Con esta contribución pretendemos, además, facilitar la labor de otros investigadores en el desarrollo de futuros trabajos y estudios vinculados a la comunicación municipal.
La entrada en el mundo de la obra narrativa audiovisual a través de Temps et récit de Paul Ricoeur
Authors: Vela Valldecabres Daniel 
Published: October 2015

En el presente estudio, exponemos la experiencia docente en la asignatura Historia del cine, por la cual se analizan películas generacionales con el método de Paul Ricoeur. Para el análisis hemos tomado la Hermenéutica de Paul Ricoeur de textos narrativos en su libro Temps et récit, y aplicado sus conclusiones a textos audiovisuales. Ricoeur establece tres fases de análisis. La primera es la precomprensión de la obra oprefiguración, por la contextualización y explicación previa a la proyección de determinadas películas, estas se vuelven más asequibles para el espectador actual. La segunda fase, laconfiguración, sería el momento en que el espectador hace suya la película al verse reflejado o no en ella. Por último, larefiguración representa el momento de la recepción y aplicación en la vida real de los modelos que se ven en pantalla.El análisis de las películas generacionales y su impacto didáctico en la formación de los alumnos a través de la asignatura Historia del cine nos ayuda a valorar cómo los alumnos pueden conocer al mismo tiempo las características estructurales y formales de una generación y activar el pensamiento crítico hacia elementos que tienen que ver con la propia estructura de entendimiento de la realidad.
El periodismo en el siglo de las redes sociales
Authors: Herrero Curiel Eva 
Published: October 2015

El siguiente artículo expone y analiza los cambios que las redes sociales han producido en la profesión periodística. Sistemas de producción, contenidos o modelos de negocio han sido los principales protagonistas de este cambio en el nuevo escenario interactivo, pero existe un actor importante que es el periodista. El trabajo de este profesional ha sido completamente transformado por las nuevas tecnologías. Acceso a la información, fuentes o sistemas de verificación se han visto modificados por el uso de las redes sociales como herramienta principal en su trabajo diario dentro de las redacciones. Es necesario investigar de qué manera están utilizando los periodistas estos Social Media y estudiar cuáles pueden ser las claves para que estas nuevas herramientas digitales se conviertan en un recurso útil para el periodista del siglo XXI.
Nuevos sistemas para la evaluación continua del rendimiento del alumnado. Estudio de caso del coaching en el grado de ingeniería forestal
Authors: González-Loureiro Miguel 
Published: October 2015

En este artículo se describe el uso del Coaching para evaluar el rendimiento de los alumnos de forma continua en el área de conocimiento de “organización de empresas” dentro del marco de una titulación de ingeniería como es el Grado en Ingeniería Forestal.En los últimos años el Coaching se está convirtiendo en una herramienta muy útil en el desarrollo profesional y personal. Por eso se incorporó esta nueva herramienta con impactos positivos tanto en la evaluación continua del rendimiento de alumnos como en la transferencia de competencias transversales en estudios universitarios de ingeniería. Así, en el nuevo marco del Espacio Europeo de Educación Superior, el profesor pasa a ser el coach, el guía en el aprendizaje de competencias dejando de lado el rol del docente como “persona que simplemente enseña”. Dichas competencias serán aplicadas por el estudiante una vez finalice su etapa en la universidad.Adicionalmente el Coaching como metodología y estrategia docente es una de las más adecuadas para facilitar la evaluación continua del alumno. Así, en el desarrollo profesional y de madurez del alumno se enfatiza el “aprender-haciendo” (learning-by-doing), con la ayuda del coach. Es muy importante en este caso el refuerzo de la función de retroalimentación.