bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coagulase‐Negative Staphylococci Isolated from Various Skin Lesions
Authors: Akiyama Hisanori , Kanzaki Hiroko , Tada Joji , Arata Jirô 
Published: April 2015

AbstractWe isolated 162 coagulase‐negative staphylococci (48: from infection, 114: from colonization) from various skin diseases between January, 1995, and January, 1998. From eighteen infected cysts, 10 Staphylococcus epidermidis strains, 3 S. capitis strains, 2 S. hominis strains, 2 S. auricularis strains, and one S. saprophyticus strain were individually detected. Similarly, from ten folliculitis lesions, 6 S. epidermidis strains, 2 S. capitis strains, and 2 S. hominis strains, and from five furuncle lesions, 3 S. lugdunensis strains, one S. epidermidis strains, and one S. hominis strain were detected, respectively. Four abscesses with mild inflammatory signs were localized on the scalp; S. epidermidis strains alone were detected from them. From two felons, one S. lugdunensis strain and one S. haemolyticus strain were detected, respectively. Staphylococus epidermidis and S. lugdunensis strains seems to be more frequently associated with skin suppurative lesions than other strains. Staphylococcus hominis strains and S. capitis strains were suggested to be potential pathogens in the initiation of suppuration in various purulent skin lesions. Among the 28 S. epidermidis strains, 13 (46.4%) were methicillin‐resistant (oxacillin. minimum inhibitory concentration ≥4 μg/ml). Twelve (29.3%) out of the other 41 coagulase‐negative staphylococci were methicillin‐resisitant. Coagulase‐positive and ‐negative staphylococci showed no differences in susceptibility tests against various antistaphylococcal agents.
Squamous Cell Carcinoma of the Skin Associated with Sarcoid Reactions in the Regional Lymph Nodes
Authors: Setoyama Mitsuru , Nishi Masayuki , Uchimiya Hiroshi , Kanzaki Tamotsu 
Published: April 2015

AbstractSarcoid reactions in lymph nodes with or without metastasis from a primary malignant neoplasm are well‐known. However, it is extremely rare to find these reactions associated with cutaneous solid tumors; only one such case has appeared in the literature. Here we describe a case of an 83‐year‐old man with cutaneous squamous cell carcinoma accompanied by sarcoid reactions and metastatic foci in the regional lymph nodes. The possibilities of systemic sarcoidosis and tuberculosis were excluded after extensive examinations specific for these diseases. Some authors regard the sarcoid reaction to be a sign of a good prognosis on the basis of studies of a few patients with solid tumors. In our case, however, the patient died of pulmonary metastasis with pleuritis carcinomatosa shortly after surgery.Systematic analysis of a sufficient number of cases should be carried out to evaluate the clinical significance of this type of reaction.
A Case of Superficial Leiomyosarcoma, and a Review of Japanese Cases
Authors: Wakasugi Shoji , Kayashima Ken‐ichi , Ono Tomomichi 
Published: April 2015

AbstractWe report a case of leiomyosarcoma in a 55‐year‐old Japanese female. She developed an erythematous nodule, 1.5 cm in diameter, on her right thigh. The totally excised specimen contained tumor cells with variously sized, chromatin‐rich nuclei. Gitter staining revealed the so‐called encased picture, immunohistopathological staining was positive for smooth muscle actin, and electron microscopy revealed filaments with focal densities. A diagnosis of leiomyosarcoma was made based on these findings, which suggested a muscle cell origin. Normal arrector pili muscles were continuous from the tumor, further suggesting the tumor's origin. The presence of Epstein‐Barr virus‐encoded RNA (EBER) was examined by in situ hybridization with negative results.
Pustular Drug Eruption Due to Bacampicilin Hydrochloride in a Patient with Psoriasis
Authors: Isogai Zenzo , Sunohara Akiyo , Tsuji Takuo 
Published: April 2015

AbstractWe report a case of pustular drug eruption due to bacampicilin hydrochloride which developed in a patient with pustular psoriasis. The patient was a 45‐year‐old Japanese woman with psoriasis which started as pustular psoriasis twenty years previously. In 1994, she developed generalized erythema with pustules accompanied by high fever and liver injury. Clinical and histological findings of this pustular eruption were different from her previous episodes of pustular psoriasis. Erythemas and pustules disappeared and her abnormal transaminase returned to normal rapidly when she discontinued bacampicilin hydrochloride. Her positive reaction to a patch test and a lymphocyte stimulation test also suggested that our case had a pustular drug eruption rather than pustular psoriasis induced by a drug.
Comparative Studies of the Detection Rates of Leishmania Parasites from Formalin, Ethanol‐fixed, Frozen Human Skin Specimens by Polymerase Chain Reaction and Southern Blotting
Authors: Uezato Hiroshi , Hagiwara Keisuke , Hosokawa Atsushi , Maruno Motoyoshi , Nonaka Shigeo , Oshiro Minoru , Nakashima Yasutsugu , Furuya Masato , Hashiguchi Yoshihisa 
Published: April 2015

AbstractIn this study, detection rates of Leishmania parasites from human skin were compared among three different types of specimens, formalin‐fixed, ethanol‐fixed, and frozen, by polymerase chain reaction (PCR) and Southern blotting. For this purpose, we used biopsy specimens collected from 19 leishmaniasis patients and performed PCR and Southern hybridization with the probe specific for Leishmania (Viannia) braziliensis complex. Among these 19, 16 specimens were from cutaneous leishmaniasis (CL), one, diffuse cutaneous leishmaniasis (DCL) and 2, mucocutaneous leishmaniasis (MCL) and were formalin‐fixed and paraffin‐embedded. The causative agents for one case of CL and one case of DCL were already identified as L. (Leishmania) complex. Six specimens of CL were preserved in 100% ethanol. Two specimens of MCL were frozen tissues. PCR using the formalin‐fixed and paraffin‐embedded specimens revealed positive bands at 70bp in 9 (47.4%) out of 19 specimens of CL, MCL and DCL. Southern blotting detected the signals in 12 (63.2%) out of the 19. PCR using the 100% ethanol‐fixed specimens revealed positive bands in 4 (66.7%) out of 6, and Southern blotting also detected the signals in 4 (66.7%) out of the 6. PCR and Southern blotting using 2 frozen specimens of MCL were always positive (100%). Although we failed to detect significant differences by Chi‐square test between the results from the formalin‐fixed, paraffin‐embedded specimens and those from 100% ethanol‐fixed ones, we concluded that ethanol‐fixed specimens, convenient for transportation and storage, would be more useful for diagnosis of leishmaniasis by PCR in a developing country.
Characteristics of UVA‐Induced Delayed Tanning: Seasonal Variation
Authors: Sakamaki Takeshi , Arai Seiichi , Kawada Akira 
Published: April 2015

AbstractWe studied seasonal variation in UVA‐induced delayed tanning and compared it to that in UVA + B‐induced delayed tanning. The minimal tanning dose (MTD) and the minimal melanogenic dose (MMD) were determined one week after UVA exposure or UVA + B exposure, respectively. The MTD determined in the summer (June to August) was significantly higher than that in winter (December to February). In addition, while MTD in the winter correlated well with skin color, there was no correlation between MTD and skin color in summer. MTD tended to correlate with self‐reported tanning history at the initiation of tanning in the winter, but they were not correlated with each other in the summer. The MMD measured in the summer was significantly lower than that in the winter. MMD correlated well with skin color in both seasons; the lighter the skin, the smaller the MMD. Also, a correlation was detected between MMD and self‐reported tanning history. These results suggest that the susceptibilities of delayed tanning to UVA and UVA + B are affected by different factors.
Management of Toenail Onychomycosis with 2% Butenafine and 20% Urea Cream: A Placebo‐controlled, Double‐blind Study
Authors: Syed Tanweer A. , Ahmadpour Oliver Abbas , Ahmad Seyed Ali , Shamsi Sadaf 
Published: April 2015

AbstractOnychomycosis is an increasingly common and recalcitrant fungal nail infection worldwide. The purpose of this placebo‐controlled, double‐blind study was to determine the clinical efficacy, chemical avulsion, and tolerability of 2% butenafine hydrochloride and 20% urea incorporated in a cream to cure toenail onychomycosis in a preselected population. Sixty patients (38M, 22F), ranging between 18 and 60 years (mean 27.4), with more than 25% involvement of the big toenail were enrolled in the study. The diagnosis of onychomycosis was established by mycologic identification and reconfirmed by positive fungal culture. A precoded 25‐g tube was randomly assigned to each patient (50 active and 10 placebo) with instructions to apply the trial medication to their infected toenail twice daily with an occlusive dressing for one week. The affected nail was removed with a nail clipper. No occlusive dressing was maintained after the initial one week regimen. To assess the chemical avulsion of the infected toenail, mycologic cure, clinical effectiveness of the treatment, and overall success, patients were examined twice a week for 16 weeks and thereafter on a weekly basis for a further 36 weeks. The treatment was well tolerated by all the patients throughout the study, with no dropouts. Marked improvement was seen in 73.3% patients after weeks 8, 16 and 24 with clinically and mycologically confirmed negative fungal culture. Code disclosure revealed that active butenafine and urea cream cured significantly more patients than placebo (88% versus 0%; p<0.0001). Of the 60 patients 91.6% reported no drug‐related adverse symptoms. Five patients reported non‐objective mild inflammation without discontinuation of the treatment. During one year follow‐up of the study phase, none of the cured patients had a relapse. In conclusion, the mycologic and overall assessment of this study demonstrate that 2% butenafine HCl and 20% urea incorporated in a cream for keratinolysis is safe to use and significantly more effective than placebo in curing big toenail onychomycosis.
A Case of Pustular Ulcerative Dermatosis of the Scalp Associated with Ulcerative Skin Lesions on the Scrotum
Authors: Sadayama Takahiro , Muramatsu Tsutomu , Shirai Toshihiko 
Published: April 2015

AbstractA 79‐year‐old man developed pustules, erosions, and ulcer with severe pain on the parietal regions of the scalp and the scrotum. In another hospital, mycobacterium was detected from the pustules of the scalp at Gaffky scale 1, and this patient was referred to our clinic. In our hospital, repeated cultures of the pus from the skin lesions of the scalp were negative for mycobacterium. Biopsy specimens obtained from the skin lesions on the scalp disclosed ulcerative granulation with infiltration of inflammatory cells. Antibiotics and antituberculotic agents were ineffective. In contrast, the ulcerative skin lesions on the scalp and scrotum responded well to topical and systemic glucocorticosteroids. Although this patient developed pustular ulcerative skin lesions not only on the scalp but also on the scrotum, we considered this case to be pustular ulcerative dermatosis of the scalp developing ulcerative skin lesions on the scrotum.
An Unusual Panniculitis Appearing in the Winter with Good Response to Tetracycline
Authors: Aroni Kyriaki , Aivaliotis Mackos , Tsele Efzossini , Charalambopoulos Dionysios , Davaris Panagiotis 
Published: April 2015

AbstractCold panniculitis is a form of physical panniculitis due to exposure of skin to severe cold. It usually appears on the cheeks of infants and children. It has also been reported on the thighs and buttocks of young females. Its clinical manifestations include red, cold, indurated plaques or nodules which appear one to three days after exposure to low temperatures and resolve spontaneously within several weeks without scarring.The histopathological picture shows a perivascular infiltrate of lymphoid and histiocytic cells at the dermal‐subcutaneous junction in the early phase of the reaction (1). After 48 to 72 hours, a well developed panniculitis appears.We report an unusual case of an adult female patient with recurrent panniculitis on her legs appearing in the winter but without any preceding repeated or prolonged exposure to cold. She responded dramatically to oral tetracycline. This drug was successful as a prophylactic agent as well.
Verruca Vulgaris Developing during Cyclosporin Therapy for Psoriasis Vulgaris
Authors: Irimajiri Junya , Yamamoto Toshiyuki , Kimura Kyoko , Yokozeki Hiroo , Nishioka Kiyoshi 
Published: April 2015

AbstractSeveral kinds of side effects have recently been reported with cyclosporin (CsA) therapy. We describe two patients with psoriasis vulgaris who developed verrucae vulgaris during systemic CsA therapy.In one case, a verrucous nodule appeared on the scalp and grew rapidly in a 54‐year‐old male with psoriasis vulgaris 29 months after starting CsA therapy. The total dose of CsA was 87.5 g, and average dose of CsA was 2 mg/kg/day.In the other case, multiple verrucae vulgaris developed on the scalp, face, knee, and inguinal region in a 54‐year‐old male. He had been given CsA for 10 months, the total dose was 45.5 g, and the average dose was 3 mg/kg/day. He also had Sjögren's syndrome. Most of the verrucae vulgaris lesions spontaneously disappeared after stopping the CsA.
A Clinical, Microbiological, and Histopathologic Study of Trichostasis Spinulosa
Authors: Chung Tae‐Ahn , Lee Jae‐Bong , Jang Ho‐Sun , Kwon Kyung‐Sool , Oh Chang‐Keun 
Published: April 2015

AbstractTrichostasis spinulosa (TSS) is a relatively common follicular disorder that can occur on the face and trunk, especially in the interscapular area. Its cause remains unclear. We examined clinically 30 patients with TSS and follicular materials extracted from each patient were examined microscopically. Bacterial culture and skin biopsy were done in 12 and 10 patients, respectively. Periodic acid Schiff (PAS) and Brown‐Brenn Gram stain were used for detection of pityrosporum (malassezia) and bacteria. The interscapular area (14/30), nose (8/30), and cheek (4/30) were common sites of TSS. Pityrosporum and bacteria in the extracted follicular material were found at the rates of 82.6% and 73.3%, respectively. In histologic examination, follicular hyperkeratosis and numerous vellus hairs enveloped within keratotic sheath were common features. Pityrosporum and bacteria were found at the rate of 70% in biopsied specimens on PAS and Brown‐Brenn Gram stain. In bacterial culture, Propionibacterium acne was most commonly identified in 75% (9 out of 12 patients). Pityrosporum and bacteria, especially Propionibacterium acne, were commonly found in the extracted follicular material and biopsied specimens. Thus, they may be related to the induction of follicular hyperkeratosis with retention of vellus hairs, and we suggest that these microorganisms may be one of the possible etiologic factors of TSS.
Effect of Nonenzymatic Glycosylation on the Titers of Circulating Autoantibodies in Pemphigus and Pemphigoid
Authors: Nakagawa Akemi , Kobayashi Nobuhiko , Yamashina Yukio , Nakatani Chie , Muramatsu Tsutomu , Mori Toshio , Shirai Toshihiko 
Published: April 2015

AbstractHyperglycemia is observed in some patients with autoimmune bullous diseases complicated by diabetes mellitus or treated with systemic corticosteroids. High concentrations of glucose can react with various proteins and change their structural and functional properties. We previously reported that nonenzymatic glycosylation of antibody can impair antigen‐antibody binding. We ascertained whether glycosylation of autoantibody decreases the autoantibody titer by examining 30 sera from patients with pemphigus and pemphigoid. Nonenzymatic glycosylation in the physiological range was induced by incubation of sera with 1650 mM D‐glucose at 4°C for 7 days. The titers of sera were determined by indirect immunofluorescence (IIF). In all cases, the immunofluorescence intensity of glycosylated sera was weaker than that of nonglycosylated sera. Glycosylated sera showed a lower antibody titer by 1 doubling dilution in 18 out of 30 cases, compared with nonglycosylated sera. The ten BP patients' sera were also analyzed by immunoblotting for reactivity with the BP180‐GST fusion proteins, SΔ1 and 4575. All BP sera reacted with SΔ1, and 5 out of 10 BP sera reacted with both SΔ1 and 4575. In all the sera that reacted only with SΔ1, the glycosylated sera showed a 1 doubling dilution decrease in autoantibody titer. Interestingly, in 4 out of 5 sera that reacted with both SΔ1 and 4575, there were no differences in the antibody titer between glycosylated and nonglycosylated sera.These results indicate the possibility of a false decrease in autoantibody titers of sera from patients with autoimmune bullous diseases complicated with hyperglycemia. Although the false decrease in titers of autoantibodies induced by nonenzymatic glycosylation is not dramatic, it must be considered in order not to underestimate the disease activity of pemphigus in such cases.
Phacomatosis Pigmentovascularis Type IIb Associated with Sturge‐Weber Syndrome and Pyogenic Granuloma
Authors: Hagiwara Keisuke , Uezato Hiroshi , Nonaka Shigeo 
Published: April 2015

AbstractA case of phacomatosis pigmentovascularis (PPV) in a 6‐year‐old girl with Sturge‐Weber syndrome, pyogenic granuloma, and other complications is described. It is relatively rare that a complete form of Sturge‐Weber syndrome was associated with PPV. A review of the literature on PPV, focusing on total number of reported cases and etiological speculations, is presented. To our knowledge, a total of 118 cases of PPV, including the present one, have been reported to date. Regardless of many speculations, the true etiology remains unknown. The average “density” of mast cells (MCs) per mm2 appearing in the central region of the pyogenic granuloma was calculated to be 86.3/mm2 and that in the adjacent nevus flammeus was 37.9/mm2. The “density” of mast cells in pyogenic granuloma separately calculated from ten other cases was 105.5 ± 28.6/mm2 (mean ± SD), compared with that in normal skin, 6.85 ± 4.9/mm2 (n=20). There was a significant difference between the two, indicating that MCs are closely associated with angiogenesis in pyogenic granuloma.
Chronic Pruritic Papular Dermatitis in Adult Men: A Variant of Prurigo
Authors: Chang Sung Nam , Kim Soo‐Chan , Chun Yoon‐Sun , Kim Kyun Tae , Ahn Sung Ku , Park Wook Hwa 
Published: April 2015

AbstractChronic recurrent pruritic papular eruptions in which a specific diagnosis cannot be established becomes a baffling experience to the dermatologist. We have met adult male patients with chronic recurrent pruritic papular eruptions, but their clinicopathological features are not described in English language textbooks. Our purpose was to study the clinical and histological features of this entity and review the various existing taxonomy. We conducted a study of 20 patients over a six year period by taking histories, performing skin biopsies, screening patch tests, and doing immunofluoresence studies. The eruptions occured exclusively in male adults and had a predilection for the trunk and proximal extremities. The lesions were characterized by severely pruritic, nonfollicular, monomorphic, erythematous urticarial papules. There was no evidence of atopic diathesis or history of insect bite. Most patients had normal levels of serum eosinophils and IgE. The predominant histopathologic finding was a presence of perivascular infiltration of mononuclear cells with eosinophils. The patients followed a chronic course of at least six months with waxing and waning; systemic corticosteroids were the only effective treatment. Finally, all other pruritic erythematous papular dermatoses were ruled out. These cases comprise a distinct entity that has previously been mentioned in a few reports. Clear definition of this entity with an appropriate designation is in order to avoid confusion among dermatologists, and we propose the disease name “chronic papular dermatitis in adult men” as a variant of prurigo.
A Clinical and Bacteriological Examination of Mycobacterium leprae in the Epidermis and Cutaneous Appendages of Patients with Multibacillary Leprosy
Authors: Hosokawa Atsushi 
Published: April 2015

AbstractIn the specimens examined at Ryukyu University Hospital, acid‐fast bacilli (AFB) were observed in the epidermis, cutaneous appendages and endotherial cells of capillaries. These specimens were taken from non‐ulcerating skin lesions of patients with multibacillary leprosies such as LL and borderline lepromatous leprosy (BL). Of the 211 specimens examined, 23 (10.9%) were AFB‐positive [AFB (+)] in the above mentioned skin regions. These AFB (+) samples were taken from nine leprosy patients; six cases (17 samples) of LL, two cases (5 samples) of BL, and one case (one sample) of BB. The AFB positive rate [AFB (+)‐rate] in the above mentioned skin regions was high in the unmedicated LL sample (50.0%, 7/14) and low in the medicated mid‐borderline leprosy (BB) samples (0.0%, 0/10). Particularly in the intraepidermal eccrine sweat duct (acrosyringium), a relatively high number of AFB were observed. The AFB (+)‐rate appears likely to be higher in non‐ulcerating skin lesions with minor inflammation or in lesions with leprosy reaction than typical skin lesions such as papules, nodules, and infiltrated punched out skin lesions. Although the possibility that viable bacilli could be excreted from non‐ulcerating skin lesions appeared to be small, these lesions were suspected of being a possible source of infection.
Generalized Atrophic Benign Epidermolysis Bullosa: A Case of Severe Hemidesmosomal Deficiency
Authors: Hashimoto Ken , Shwayder Tor 
Published: April 2015

AbstractA case of generalized atrophic benign epidermolysis bullosa was followed from birth to 14 years of age. Electron microscopy of the blistered skin showed either the absence or greatly reduced hemidesmosomes of a very poorly developed type. In perilesional skin, the frequency of hemidesmosomes was 1/3 to 1/2 of the normal controls. These hemidesmosomes were small and lacked normal ultrastructures. Anchoring filaments did not cluster underneath them, and the anchoring fibrils were also diminished. In view of the published data correlating the genotype to the phenotype, this patient seems to have a homozygous nonsense mutation of the COL17A1 gene encoding collagen XVII. An accumulation of data may eventually enable electron microscopists to estimate the types of gene mutation.
Basosquamous Carcinoma after Ultraviolet Therapy with Artificial Sunlight
Authors: Miyamoto Hideaki , Okajima Mitsuya 
Published: April 2015

AbstractWe report a 63‐year‐old Japanese man with basosquamous carcinoma developing after ultraviolet therapy with artificial sunlight. Because he had had an eczematpus lesion on the right leg five years previously, he had received ultraviolet therapy with artificial sunlight from a mercury‐quartz lamp for a total of 30 sessions over a 3‐year period. Physical examination revealed an irregular erythematous plaque measuring 5 times 6 cm, with slight eruptions and erosions, on the right leg. A brownish plaque, measuring 9 times 12 cm, surrounded the erythematous plaque. Histopathologically, in the nests of atypical cells in the dermis, nuclear palisading was present at the edge of each tumor cluster. Adjacent clusters exhibited transitions to squamous cells and contained horn pearls. Most of the cells of the epidermis and adnexa were atypical, but cells of the normal adnexal epithelium extended over the atypical cells of the epidermis in an umbrella‐like fashion in other lesions. We believe that, in the present case, artificial sunlight therapy caused solar keratosis and that this developed into basosquamous carcinoma. To our knowledge, skin cancer developing after ultraviolet therapy with artificial sunlight without PUVA has not previously been reported in the English literature.