bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular dissection of the parental contribution in Paeonia Itoh hybrids
Authors: Hong Conghao , Zhao Yingying , Qiao Meiyu , Huang Ziteng , Wei Lan , Zhou Qingqing , Lu Wanqing , Sun Guorun , Huang Zhimin , Gao Hongbo 
Published: August 2024

Abstract
Hybrid breeding between herbaceous peonies (the maternal parent) and tree peonies (the paternal parent) results in Paeonia Itoh hybrids (Itoh peonies), a triploid species that combines advantageous traits from both parental species, thus offering great economic value. However, the exact genetic contribution of the two parents is unclear. In this study, we introduce a straightforward approach utilizing heterozygous single-nucleotide polymorphisms (SNPs) and Sanger sequencing of targeted gene fragments to trace the original bases back to their parents in Itoh peonies. Our results indicate that in triploid Itoh peonies, only one set of genes is derived from herbaceous peonies, and two sets of genes are derived from the tree peonies. Notably, the presence of three distinct bases of heterozygous SNPs across multiple Itoh cultivars suggests that the gametes from the paternal parents carry two sets of heterozygous homologous chromosomes, which could be due to Meiosis I failure during gamete formation. To validate our method's effectiveness in parentage determination, we analyze two Itoh hybrids and their parents, confirming its practical utility. This research presents a method to reveal the parental genetic contribution in Itoh peonies, which could enhance the efficiency and precision of hybrid breeding programs of triploids in Paeonia and other plant species.
Policymaking and the role of government in promoting public health during Covid-19 in Indonesia?
Authors: Abdillah Abdillah , Widianingsih Ida , Buchari Rd Ahmad , Nurasa Heru 
Published: August 2024

Abstract
The global COVID-19 pandemic has significantly impacted inadequate physical activity, which has an impact on public health. Government policies are critical in supporting public health and encouraging physical activity during COVID-19. In addition, collaboration between public and health authorities, influencers, and governments is essential in implementing public education campaigns to promote physical activity and healthy behaviors. In Indonesia, strategies to encourage physical activity among children can include home-based physical activity programs, online physical education classes, and outdoor activities that adhere to social distancing guidelines.
Cryopreserved apoptotic mesenchymal stromal cells retain functional efficacy in suppressing an allergic inflammation in a murine model
Authors: Amison Richard T , Cheung Tik S , Giacomini Chiara , Riffo-Vasquez Yanira , Galleu Antonio , Savoldelli Roberto , Hicks Ryan , Kozlowska Anna , Dazzi Francesco 
Published: August 2024

Abstract
Mesenchymal stromal cell (MSC) apoptosis is required for in vivo immunosuppression. However, the induction of apoptosis is heavily dependent on the recipient’s immune system. In graft-versus-host disease (GvHD), patients who fail to respond to MSCs are in fact those whose immune cells are unable to induce MSC apoptosis ex vivo. The information is critical to explain why responses in clinical trials vary even though the same sources of MSC products are infused. More importantly, it highlights the need for an alternative MSC treatment for the nonresponders. By using a mouse model of ovalbumin (OVA)-induced allergic inflammation, we demonstrated that we could generate apoptotic MSCs (ApoMSCs) in vitro and use them to successfully reduce allergic airway inflammation. In order to address the logistics of their potential future clinical application, we have shown that ApoMSCs could be cryopreserved without impairing efficacy compared to freshly generated ApoMSCs. We have also highlighted that MSCs need to undergo complete apoptosis before cryopreservation to retain their immunosuppressive activity. The cryopreserved ApoMSCs could serve as a potential future off-the-shelf cellular product, in particular for patients who suffer from inflammatory conditions yet do not harbor the immune capacity to induce MSC apoptosis in vivo. Our data provide proof-of-concept that under laboratory conditions, ApoMSCs can be successfully frozen and thawed without affecting their anti-inflammatory activity, as tested in a murine model of allergic inflammation.
PhyloJunction: A Computational Framework for Simulating, Developing, and Teaching Evolutionary Models
Authors: Mendes Fábio K , Landis Michael J 
Published: August 2024

Abstract
We introduce PhyloJunction, a computational framework designed to facilitate the prototyping, testing, and characterization of evolutionary models. PhyloJunction is distributed as an open-source Python library that can be used to implement a variety of models, thanks to its flexible graphical modeling architecture and dedicated model specification language. Model design and use are exposed to users via command-line and graphical interfaces, which integrate the steps of simulating, summarizing, and visualizing data. This article describes the features of PhyloJunction—which include, but are not limited to, a general implementation of a popular family of phylogenetic diversification models—and, moving forward, how it may be expanded to not only include new models, but to also become a platform for conducting and teaching statistical learning.
Toxicological evaluation of copper oxide nanoparticles following their intraperitoneal injection to Wistar rats
Authors: Shareef Nisha , Abid Shairyar , Amir Aqsa , Ismail Amir , Ullah Abd , Ahmad Adnan , Ibenmoussa Samir , Bin Jardan Yousef A , Bourhia Mohammed , Ibrahim Ather , Iqbal Furhan 
Published: August 2024

Abstract

Background
Copper oxide (Cu2O) nanoparticles (CO NPs) are in extensive use during our everyday life as antimicrobial agent, lubricant, in manufacturing electrodes of lithium ion batteries as well as for photo catalytic degradation of organic pollutants. Due to extensive and diverse use Cu2O NPs, they are likely to accumulate in the environment and to affect the live forms. Present investigation was aimed to report the biocompatibility of CO NPs in Wistar rats in sex specific manner. CO NPs, having average diameter of 14.06 nm, were synthesized by co-precipitation method and scanning electron microscopy and X ray diffraction were used for their characterization.

Methods
For 14 consecutive days, Wistar rats (6 weeks old) of both sexes were intraperitoneally injected with 10 mg/mL saline/Kg body weight of CO NPs, while the control groups intraperitoneally received saline solution for same duration. Behavioral tests (open field and novel object recognition), complete blood count, selected biomarkers of oxidative stress and Copper concentration in brain and liver were determined in all subjects.

Results
High mortality rates [male 40% and female 60%] were observed in rats exposed to CO NPs. A sever decrease in body weight was also observed in both male and female rats exposed to CO NPs. Female rats treated with CO NPs spent significantly more time with novel object as compared to control [P = 0.05] during second trial of novel object test. CO NPs treated female rats had higher mean corpuscular hemoglobin [P &lt; 0.001] levels and Copper concentration in liver [P = 0.04] than control. Male rats exposed to CO NPs had significantly higher mean corpuscular volume [P = 0.02] and superoxide dismutase [SOD] [P = 0.04] in lungs than their control group. All other studied parameters non significantly varied upon comparison between CO NPs treated and untreated rats of both sex.

Conclusion
In conclusion, we are reporting that intraperitoneal injections of CO NPs for 14 days can disturb complete blood count and biomarkers of oxidative stress in lungs of Wistar rats.
The radiation of Austral teals (Aves: Anseriformes) and the evolution of flightlessness
Authors: Rosinger Hanna S , Kardailsky Olga , Kennedy Martyn , Spencer Hamish G , Steiner Florian M , Schlick-Steiner Birgit C , Rawlence Nicolas J , Knapp Michael 
Published: August 2024

Abstract
The origin and evolution in the Southern Hemisphere of the Austral teals, consisting of the grey-teal and brown-teal species complexes, remains poorly understood owing to limited molecular data. With the group containing multiple independent examples of flight loss, understanding the evolutionary history of the group is of significant interest for functional genomic studies into the evolution of flightlessness. Here, we present the first whole-mitogenome-based phylogeny of the Austral teals. We show that the group diverged from a common ancestor with mallards in the late Miocene and soon after radiated into the brown-teal and grey-teal lineages, in addition to the widely distributed pintails and green-winged teals. The brown-teal species complex, which includes the volant brown teal, the flight-impaired, sub-Antarctic Auckland Island teal, and the flightless, Chatham Island and sub-Antarctic Campbell Island teals, radiated within the past 0.9–1.8 Myr. The divergence of the extinct Chatham Island teal and the colonization of the Auckland and Campbell Islands occurred from mainland New Zealand. Morphological changes towards flightlessness are also present in the volant brown teal on mainland New Zealand, suggesting that this group was on the pathway to flightlessness, a trend that accelerated in some insular island lineages.
New insights into the evolution and biogeography of freshwater planarians on islands in the Tyrrhenian Sea, Western Mediterranean Basin, with the integrative description of a new endemic species from Corsica (Platyhelminthes: Tricladida: Dugesia)
Authors: Dols-Serrate Daniel , Stocchino Giacinta Angela , Nuin-Villabona Paula , Sluys Ronald , Riutort Marta 
Published: August 2024

Abstract
A recent study on the freshwater planarian fauna of Corsica and Sardinia established that the formerly presumed single species Dugesia benazzii subsumed a complex of species. In that study, a thorough integrative taxonomic approach, combining molecular, morphological, and karyological data, uncovered the presence of two new endemic species. For the present study, additional samplings were conducted on Corsica, covering several new localities. The data obtained were added to our previous datasets, which included information on specimens collected from both islands. By taking a similar integrative approach to the prior study, we here describe another new endemic species from Corsica, Dugesia xeropotamica. Although our results support the separate status of this new species, it also turned out that the evolutionary relationships among species of the Corso-Sardinian clade remain unclear, because the relationships are not well resolved. Additionally, we corroborate the presence of Dugesia mariae on the Tyrrhenian islets of Montecristo and Molara, representing new records for this group of Dugesia species in the region of the Tyrrhenian Sea. In all, our findings indicate that the evolutionary and biogeographical history of Dugesia species in this region of the Western Mediterranean is more intricate than previously thought.
Quality of Reporting on Anastomotic Leaks in Colorectal Cancer Trials: A Systematic Review
Authors: Heuvelings Danique J.I. , Mollema Omar , van Kuijk Sander M.J. , Kimman Merel L. , Boutros Marylise , Francis Nader , Bouvy Nicole D. , Sylla Patricia ,   On behalf of the CoReAL Collaborative
Published: August 2024

BACKGROUND:
Although attempts have been made in the past to establish consensus regarding the definitions and grading of the severity of colorectal anastomotic leakage, widespread adoption has remained limited.

OBJECTIVE:
A systematic review of the literature was conducted to examine the various elements used to report and define anastomotic leakage in colorectal cancer resections.

DATA SOURCES:
A systematic review was conducted using the PubMed, Embase, and Cochrane Library Database.

STUDY SELECTION:
All published randomized controlled trials, systematic reviews, and meta-analyses containing data related to adult patients undergoing colorectal cancer surgery and reporting anastomotic leakage as a primary or secondary outcome, with a definition of anastomotic leakage were included.

MAIN OUTCOME MEASURES:
Definitions of anastomotic leakage, clinical symptoms, radiological modalities and findings, findings at reoperation, and grading terminology or classifications for anastomotic leakage.

RESULTS:
Of the 471 articles reporting anastomotic leakage as a primary or secondary outcome, a definition was reported in 95 studies (45 randomized controlled trials, 13 systematic reviews, and 37 meta-analyses) involving a total of 346,140 patients. Of these 95 articles, 68% reported clinical signs and symptoms of anastomotic leakage, 26% biochemical criteria, 63% radiological modalities, 62% radiological findings, and 13% findings at reintervention. Only 45% (n = 43) of included studies reported grading of anastomotic leakage severity or leak classification, and 41% (n = 39) included a time frame for reporting.

LIMITATIONS:
There was a high level of heterogeneity between the included studies.

CONCLUSIONS:
This evidence synthesis confirmed incomplete and inconsistent reporting of anastomotic leakage across the published colorectal cancer literature. There is a great need to develop and implement a consensus framework for defining, grading, and reporting anastomotic leakage.

REGISTRATION:
Prospectively registered at PROSPERO (ID 454660).
Leveraging machine learning to enhance postoperative risk assessment in coronary artery bypass grafting patients with unprotected left main disease: a retrospective cohort study
Authors: Elmahrouk Ahmed , Daoulah Amin , Panduranga Prashanth , Rajan Rajesh , Jamjoom Ahmed , Kanbr Omar , Alzahrani Badr , Qutub Mohammed A. , Yousif Nooraldaem , Chachar Tarique Shahzad , Elmahrouk Youssef , Alshehri Ali , Hassan Taher , Tawfik Wael , Haider Kamel Hazaa , Abohasan Abdulwali , Alqublan Adel N. , Alqahtani Abdulrahman M. , Ghani Mohamed Ajaz , Al Nasser Faisal Omar M. , Almahmeed Wael , Ghonim Ahmed A. , Hashmani Shahrukh , Alshehri Mohammed , Elganady Abdelmaksoud , Shawky Abeer M. , Fathey Hussien Adnan , Abualnaja Seraj , Noor Taha H. , Abdulhabeeb Ibrahim A. M. , Ozdemir Levent , Refaat Wael , Kazim Hameedullah M. , Selim Ehab , Altnji Issam , Ibrahim Ahmed M. , Alquaid Abdullah , Arafat Amr A. 
Published: August 2024

Background:
Risk stratification for patients undergoing coronary artery bypass surgery (CABG) for left main coronary artery (LMCA) disease is essential for informed decision-making. This study explored the potential of machine learning (ML) methods to identify key risk factors associated with mortality in this patient group.

Methods:
This retrospective cohort study was conducted on 866 patients from the Gulf Left Main Registry who presented between 2015 and 2019. The study outcome was hospital all-cause mortality. Various machine learning models [logistic regression, random forest (RF), k-nearest neighbor, support vector machine, naïve Bayes, multilayer perception, boosting] were used to predict mortality, and their performance was measured using accuracy, precision, recall, F1 score, and area under the receiver operator characteristic curve (AUC).

Results:
Nonsurvivors had significantly greater EuroSCORE II values (1.84 (10.08–3.67) vs. 4.75 (2.54–9.53) %, P<0.001 for survivors and nonsurvivors, respectively). The EuroSCORE II score significantly predicted hospital mortality (OR: 1.13 (95% CI: 1.09–1.18), P<0.001), with an AUC of 0.736. RF achieved the best ML performance (accuracy=98, precision=100, recall=97, and F1 score=98). Explainable artificial intelligence using SHAP demonstrated the most important features as follows: preoperative lactate level, emergency surgery, chronic kidney disease (CKD), NSTEMI, nonsmoking status, and sex. QLattice identified lactate and CKD as the most important factors for predicting hospital mortality this patient group.

Conclusion:
This study demonstrates the potential of ML, particularly the Random Forest, to accurately predict hospital mortality in patients undergoing CABG for LMCA disease and its superiority over traditional methods. The key risk factors identified, including preoperative lactate levels, emergency surgery, chronic kidney disease, NSTEMI, nonsmoking status, and sex, provide valuable insights for risk stratification and informed decision-making in this high-risk patient population. Additionally, incorporating newly identified risk factors into future risk-scoring systems can further improve mortality prediction accuracy.
A CARE-compliant article: A case report of retroperitoneal endometrial stromal sarcoma with multiple pulmonary metastases and literature review
Authors: Fang Yaoyao , Zhou Xiaoli , Bai Jing , Lin Qiuju , Zheng Yingchun , Bo Sijia , Sui Lei , Zhu Ting , Bai Nuo , Sun Li 
Published: August 2024

Rationale:
Endometrial stromal sarcoma is an extremely rare mesenchymal neoplasm occurring in the extrauterine. Retroperitoneal endometrial stromal sarcoma with multiple pulmonary metastases, in particular, is extremely rare.

Patient concerns:
Forty-seven-year-old woman (gravida 3, para 2), was referred to our institution with complaints of fever.

Diagnoses:
Ultrasound and computed tomographic imaging of the abdomen identified the presence of masses in the pelvic region. Additionally, computed tomographic scans and X-ray evaluations of the thorax detected dispersed masses in both the lungs and pelvic area. Histopathological analysis of the pelvic region indicated the presence of low-grade endometrial stromal sarcoma. A computed tomography-guided pneumocentesis was conducted to further characterize the pulmonary lesions, confirming the diagnosis of low-grade endometrial stromal sarcoma.

Interventions:
The patient underwent tumor resection, subsequent treatment with Medroxyprogesterone acetate for 6 months, received microwave ablation for multiple lung metastases, PARP1 inhibitor for 4 courses, and has been undergoing chemotherapy (epirubicin/ifosfamide) up to the present time.

Outcomes:
Partial remission was obtained after the above treatment and this patient is now still alive maintaining for 3 years.

Lessons:
The identification and management of this disease remain a significant challenge due to its low prevalence. Further research involving a larger number of cases is necessary to ensure consistency in diagnosis and to establish effective treatment guidelines.
Levothyroxine sodium tablets reversed Hashimoto thyroiditis-induced kidney injury, muscle injury, and lipid metabolism disorder: A case report and literature review
Authors: Li Xuesong , Cao Chenxiang 
Published: August 2024

Rationale:
Hashimoto thyroiditis (HT), a common cause of hypothyroidism, has shown an increasing incidence in recent years, particularly among women. In addition to the common complications such as lipid metabolism disorders, patients with HT may also experience some serious complications, acute kidney injury and severe muscle damage for instance. This article explored the effectiveness of levothyroxine sodium tablets (L-T4) replacement therapy in severe complications of hypothyroidism, including treatment dosage, duration of complication recovery, and whether additional treatment is needed.

Patient concerns, Diagnoses, and Interventions:
We described a case of a 52-year-old woman with HT who exhibited kidney injury, muscle injury, and lipid metabolism disorders. The increased levels of serum creatinine, creatine kinase, cholesterol, triglyceride, low density lipoprotein cholesterol, high density lipoprotein cholesterol, and the decreased levels of estimated glomerular filtration rate were obviously observed. This patient was started on L-T4 (75 and 100 µg, alternate).

Outcomes and Lessons:
Following a two-month treatment, the serum creatine kinase level decreased to within normal range. The estimated glomerular filtration rate level was restored, and the serum creatinine level was down-regulated, although slightly higher than the normal range. L-T4 partially reversed HT-induced the disorders of muscle, renal function, and lipid profile of this patient and remarkably alleviated her HT-related symptoms.
The role of geriatric assessment in the care of older adults undergoing urologic cancer surgery
Authors: Wingfield Sarah A. 
Published: August 2024

Purpose of review
Older adults undergoing urologic cancer surgery have unique needs and require unique risk assessment and management. This review will discuss recent literature on brief screening tools to identify high risk older adults in the preoperative period and the role of comprehensive geriatric assessment (CGA) in identifying and addressing geriatric vulnerabilities for older adults undergoing urologic cancer surgery.

Recent findings
Frailty screening tools such as the G8 can be used to identify patients who are at an increased risk of adverse postoperative outcomes such as postoperative complications and prolonged length of stay. CGA can provide more detailed information about geriatric syndromes prior to urologic cancer surgery.

Summary
Screening tools for geriatric vulnerabilities and CGA are valuable tools for the urologist in identifying high-risk older adults, counseling patients on perioperative risk and addressing vulnerabilities prior to surgery. Making health system-wide changes can allow this optimal practice to reach more older surgical patients.
A Rare Complication in the Oral Local Infiltration Anesthesia
Authors: Yang Sisi , Zhou Hailing , Dai Yongbo 
Published: August 2024

The present report describes a rare complication named Kuhn anemia, which happened during local infiltration anesthesia of maxillary wisdom tooth. A 24-year-old male was referred for residual crown of left maxillary wisdom tooth and therefore required extraction. Oral examination revealed a buccal impacted left maxillary wisdom tooth (28), severe caries affecting the dental pulp, and pain with percussion. This case was diagnosed as impacted wisdom tooth 28 and pulpitis. Thus, the authors performed an extraction of the 28. After injection of articaine hydrochloride (68 mg:1.7 mL) containing epinephrine (1:100,000) as local anesthetic, the patient felt pain and heat in the left cheek, and an irregularly shaped pale area appeared on the left cheek. The authors stopped the surgery and comforted him. After a 30-minute break, the cheek turned normal, and abnormal sensation was no longer felt.