bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clonal Propagation of Turmeric (Curcuma longa) and Confirmation of Genetic Fidelity of the Micropropagated Shoots by RAPD Markers
Authors: Rani Moli , Miah Md Abdul Halim , Hasan Md Toufiq , Rashid Md Harun-or , Yasmin Sabina , Haque Md Shahidul 
Published: July 2024

Turmeric (Curcuma longa) is used as a spice and has excellent medicinal value. However, the field multiplication rate of turmeric plants is not satisfactory. In vitro methods could be a feasible alternative to vegetative propagation in the field. This study was aimed at developing a suitable protocol for the clonal propagation of turmeric confirming the genetic fidelity of the in vitro regenerated plantlets. The explants of shoot bud (full, half, and quarter), root, rhizome, and leaf were cultivated on MS medium with BAP, NAA, and Kinetin. Cultured shoot buds showed a higher regeneration frequency and shoots per explant than those obtained from other explants. The highest shoot regeneration was 72.49% from shoot buds, followed by rhizome (68.76%) and the lowest was 53.93 % from ¼ shoot buds. There were significant effects of growth regulator combinations and concentrations in shoot regeneration from shoot bud explant. The highest shoot regeneration per shoot bud explant was 4.42 with BAP at 20.0 mg/l and NAA at 0.5 mg/l at 24 weeks. The best rooting response with 9.75 roots per explant was reported with 1.0 mg/l BAP with 1.0 mg/l NAA. The rooted plants were successfully transferred to pots for their acclimatization. Both the micropropagated shoots and their mother plant produced monomorphic bands with the six RAPD markers that confirm the genetic fidelity of micropropagated clones. These results showed that in vitro raised plantlets of turmeric had no risk of somaclonal variations and were found to be true-to-type in nature over the culture period. The protocol developed through this investigation has the potential application for the rapid multiplication of turmeric plants.
Plant Tissue Cult. & Biotech. 34(1): 55-69, 2024 (June)
Práticas monstruosas na invenção de um mundo para além do humano (um laboratório de dança e imaginação)
Authors: Marinelli Princesa Ricardo , MATHEUS LOPES MACHADO Gabriel 
Published: July 2024

O artigo trata de um compartilhamento de metodologias e táticas de criação desenvolvidas por meio da residência Práticas monstruosas na invenção de um mundo para além do humano (um laboratório de dança e imaginação), que aconteceu dentro da programação do Seminário Internacional de Arte e Ecologia - Políticas da Existência pelo Programa de Pós-graduação em Artes da Cena - UFRJ em parceria com o MAR - Museu de Arte do Rio. Por meio de quatro eixos investigativos a proposta visa um deslocamento da noção de humanidade construída por meio do pensamento/tecnologia moderna para um olhar atento para outras formas de vida e de alianças entre seres humanos e não humanos, materialidades diversas e hibridismos. A imaginação enquanto fisicalidade possível para criações em dança.
Predicting behavior in extinct reptiles from quantitative analysis of trigeminal osteological correlates
Authors: Lessner Emily J. , Xu Xing , Young Bruce A. , Echols M. Scott , Jones Marc E. H. , Elsey Ruth M. , Holliday Casey M. 
Published: July 2024

IntroductionVertebrate sensory systems are in close contact with surrounding tissues, often leaving bony signatures behind. These bony features are the keys to assessing variation in sensory systems in fossil taxa. The trigeminal sensory system (e.g., trigeminal ganglion, ophthalmic, maxillary, and mandibular divisions) has osteological correlates throughout the skull, including the braincase (e.g., trigeminal fossa, prootic notch, ophthalmic and maxillomandibular foramina) and rostrum (e.g., mandibular canal, neurovascular foramina).MethodsHere we measured and compared these features among a morphologically, phylogenetically, and ecologically diverse sample of sauropsids to determine strength of osteological correlates and to explore ecomorphological trends. We determined several suitable osteological correlates for trigeminal soft tissue features and discounted foramen count alone as a suitable osteological correlate. However, when size was accounted for, foramen count becomes a useful indicator of sensory ecology.Results and discussionAmong extant taxa, those engaging in tactile sensory behaviors with the face exhibit relatively larger trigeminal tissues and osteological correlates than those not engaging in tactile sensory behaviors. Though patterns are unclear among several clades, both relative feature sizes and models used to predict sensory capacity reveal a trend of increasing tactile sensitivity along the pseudosuchian lineage. Overall, a quantitative assessment of ecomorphological trends of trigeminal osteological correlates proves informative for the hypotheses of sensory behavior in extinct taxa and supports the use of similar assessment methods for other osteological correlates.
The treatment of bacterial biofilms cultivated on knee arthroplasty implants using the bioelectric effect
Authors: Tamimi Iskandar , Gasca María , Halbardier Alexandra , Martin Sergio , Martin Caballero Gregorio , Lucena Serrano Cristina , Martin Elena , Tamimi Faleh , González-Quevedo David , García de Quevedo David , Sobrino Beatriz , Palop Begoña , Guerado Enrique , Pérez Lara Almudena , Urdiales Cristina , Gómez de Gabriel Jesús Manuel 
Published: July 2024

Introduction: The formation of bacterial biofilms on knee arthroplasty implants can have catastrophic consequences. The aim of this study was to analyze the effectiveness of the bioelectric effect in the elimination of bacterial biofilms on cultivated knee arthroplasty implants.Methods: A novel device was designed to deliver a bioelectric effect on the surface of knee arthroplasty implants. 4-femoral prosthetic implants were cultivated with a staphylococcus aureus inoculum for 15 days. The components were divided into four different groups: A (not treated), B (normal saline 20-minutes), C (bioelectric effect 10-minutes), D (bioelectric effect 20-minutes). The implants were sonicated, and the detached colonies were quantified as the number of colony-forming unit (CFUs). The implants were sterilised and the process was repeated in a standardized manner four more times, to obtain a total of five samples per group.Results: The number of the CFUs after a 10-minute exposure to the bioelectric effect was of 208.2 ± 240.4, compared with 6,041.6 ± 2010.7 CFUs in group A, representing a decrease of 96.5% ± 4.3 (p = 0.004). And a diminution of 91.8% ± 7.9 compared with 2,051.0 ± 1,364.0 CFUs in group B (p = 0.109). The number of bacterial colonies after a 20-minute exposure to the bioelectric effect was 70 ± 126.7 CFUs, representing a decrease of 98.9% ± 1.9 (p = 0.000) compared with group A. And a decrease of 97.8% ± 3.0 (p = 0.019) compared with group B.Conclusions: The bioelectric effect was effective in the elimination of bacterial biofilm from knee arthroplasty implants. This method could be used in the future as part of conventional surgical procedures.
Case Report: Persistent residual shunt after a first percutaneous PFO closure followed by minimally invasive surgical failure: third time is a charm
Authors: Onorato Eustaquio M. , Alamanni Francesco , Monizzi Giovanni , Mastrangelo Angelo , Bartorelli Antonio Luca 
Published: July 2024

BackgroundEven though the optimal management of a moderate or large residual shunt following patent foramen ovale (PFO) closure is open to question, recent data confirmed that it is associated with an increased risk of stroke recurrence.Case summaryA 48-year-old woman, a migraineur with visual aura, was diagnosed with a PFO associated with a huge multifenestrated atrial septal aneurysm (mfASA) and a moderate right-to-left shunt, detectable only after a Valsalva maneuver on contrast-transthoracic echocardiography. Brain magnetic resonance imaging showed a 1-mm silent white matter lesion in the right frontal lobe. Although the indication was not supported by guidelines, a transcatheter PFO closure was performed at another center with implantation of a large, equally sized, double-disc device (Figulla UNI 33/33 mm). At 6-month follow-up, a 2D/3D transesophageal echocardiography (TEE) color Doppler showed incorrect orientation of the device, which was not parallel to the interatrial septum, with two discs failing to capture the aortic muscular rim and partially protruding in the right atrium; furthermore, a 4 mm × 7 mm ASA fenestration was documented with a residual bidirectional shunt. Thereafter, the same team performed a minimally invasive cardiac surgery under femoro-femoral cardiopulmonary bypass; however, the procedure proved ineffective and was complicated by postoperative pericarditis with pericardial effusion, requiring further rehospitalization 1 month later due to persistent pericarditis, bilateral pleuritis, phrenic nerve palsy, and atrial flutter, which was treated with amiodarone. The patient asked for a second opinion, and our multidisciplinary heart team decided to offer a percutaneous redo intervention. An uneventful implantation of a regular PFO occluder (Figulla Flex II 16/18 mm) across the septal defect was performed successfully. Twelve-month follow-up with 2D TTE color Doppler and contrast transcranial Doppler showed correct position and good interaction between the two devices, with no residual shunt.DiscussionIn addition to the incorrect indication for PFO closure and the failure of minimally invasive surgery, the procedural mishap in this case could have been due to the inappropriate implantation of the first large device within the tunnel. It would have been better to deploy the same large device in the most central fenestration, covering the PFO and a greater part of the remaining mfASA at the same time.
The 3-year follow-up of a fully biodegradable implantable device closure for perimembranous ventricular septal defects in children using echocardiography
Authors: Cong Juan , Cui Cunying , Huang Danqing , Wang Ying , Liu Sifan , Song Shubo , Fan Taibing 
Published: July 2024

ObjectsThe aim of this study was to investigate the morphologic changes of a novel fully biodegradable implantable device after closing a perimembranous ventricular septal defect (Pm-VSD) and to evaluate the effect of the occluder on the myocardial function in patients during a 3-year follow-up period.MethodsOne-year, 2-year, and 3-year follow-ups were carried out after implantation with a total of 30 Pm-VSD patients who had successful closure by the fully biodegradable occluder. In total, 30 healthy children were enrolled as controls. At discharge and at every follow-up visit, the lengths of the left and right discs of the novel device were measured in the apical three- and four-chamber as well as short-axis views. At the end of the follow-up, using three-dimensional speckle-tracking conditions, the values of myocardial deformation, including global longitudinal strain, global circumferential strain, and global area strain, were acquired.ResultsThe fully bioabsorbable double-disc occluder gradually decreased over time and was eventually invisible under echocardiographic scanning during the follow-up (p &lt; 0.05). At the end of the third year, there were no significant differences in the myocardial deformation parameters between the cases implanted with the novel devices and the controls; no significant differences were found between the basal segments of the ventricle septa and that of the left ventricle (LV) free wall among the patients who completed the Pm-VSD closure using the fully biodegradable occluder (p &gt; 0.05).ConclusionThe novel fully biodegradable occluder is a safe, effective, and perfect alternative for the treatment of VSD. Echocardiography plays a crucial role in the follow-up of this new type of occluder implantation.
Derivation and validation of the first web-based nomogram to predict the spontaneous pregnancy after reproductive surgery using machine learning models
Authors: Liu Zhenteng , Wang Meimei , He Shunzhi , Wang Xinrong , Liu Xuemei , Xie Xiaoshi , Bao Hongchu 
Published: July 2024

ObjectiveInfertility remains a significant global burden over the years. Reproductive surgery is an effective strategy for infertile women. Early prediction of spontaneous pregnancy after reproductive surgery is of high interest for the patients seeking the infertility treatment. However, there are no high-quality models and clinical applicable tools to predict the probability of natural conception after reproductive surgery.MethodsThe eligible data involving 1013 patients who operated for infertility between June 2016 and June 2021 in Yantai Yuhuangding Hospital in China, were randomly divided into training and internal testing cohorts. 195 subjects from the Linyi People’s Hospital in China were considered for external validation. Both univariate combining with multivariate logistic regression and the least absolute shrinkage and selection operator (LASSO) algorithm were performed to identify independent predictors. Multiple common machine learning algorithms, namely logistic regression, decision tree, random forest, support vector machine, k-nearest neighbor, and extreme gradient boosting, were employed to construct the predictive models. The optimal model was verified by evaluating the model performance in both the internal and external validation datasets.ResultsSix clinical indicators, including female age, infertility type, duration of infertility, intraoperative diagnosis, ovulation monitoring, and anti-Müllerian hormone (AMH) level, were screened out. Based on the logistic regression model’s superior clinical predictive value, as indicated by the area under the receiver operating characteristic curve (AUC) in both the internal (0.870) and external (0.880) validation sets, we ultimately selected it as the optimal model. Consequently, we utilized it to generate a web-based nomogram for predicting the probability of spontaneous pregnancy after reproductive surgery. Furthermore, the calibration curve, Hosmer–Lemeshow (H-L) test, the decision curve analysis (DCA) and clinical impact curve analysis (CIC) demonstrated that the model has superior calibration degree, clinical net benefit and generalization ability, which were confirmed by both internal and external validations.ConclusionOverall, our developed first nomogram with online operation provides an early and accurate prediction for the probability of natural conception after reproductive surgery, which helps clinicians and infertile couples make sensible decision of choosing the mode of subsequent conception, natural or IVF, to further improve the clinical practices of infertility treatment.
A novel mitochondrial function-associated programmed cell death-related prognostic signature for predicting the prognosis of early breast cancer
Authors: Wang Jian , Jiang Haiming 
Published: July 2024

Purpose: To screen mitochondrial function-associated PCD-related biomarkers and construct a risk model for predicting the prognosis of early breast cancer.Methods: Data on gene expression levels and clinical information were obtained from the TCGA database, and GSE42568 and GSE58812 datasets were obtained from GEO database. The mitochondrial function-associated programmed cell death (PCD) related genes in early breast cancer were identified, then LASSO logistic regression, SVM-RFE, random forest (RF), and multiple Cox logistic regression analysis were employed to construct a prognostic risk model. Differences in immune infiltration, drug sensitivity, and immunotherapy response were evaluated between groups. Lastly, the qRT-PCR was employed to confirm the key genes.Results: Total 1,478 DEGs were screened between normal and early breast cancer groups, and these DEGs were involved in PI3K-Akt signaling pathway, focal adhesion, and ECM-receptor interaction pathways. Then total 178 mitochondrial function-associated PCD related genes were obtained, followed by a four mitochondrial function-associated PCD related genes prognostic model and nomogram were built. In addition, total 2 immune checkpoint genes were lowly expressed in the high-risk group, including CD47 and LAG3, and the fraction of some immune cells in high- and low-risk groups had significant difference, such as macrophage, eosinophil, mast cell, etc., and the Top3 chemotherapeutics with significant differences were included FH535, MK.2206, and bicalutamide. Finally, the qRT-qPCR results shown that the CREB3L1, CAPG, SPINT1 and GRK3 mRNA expression were in line with the bioinformatics analysis results.Conclusion: Four mitochondrial function-associated PCD-related genes were identified, including CREB3L1, CAPG, SPINT1, and GRK3, and the prognostic risk model and nomogram were established for predicting the survival of early breast cancer patient. The chemotherapeutics, containing FH535, MK.2206, and bicalutamide, might be used for early breast cancer.
Morphologic, cytometric, quantitative transcriptomic and functional characterisation provide insights into the haemocyte immune responses of Pacific abalone (Haliotis discus hannai)
Authors: Ma Zeyuan , Wu Yunlong , Zhang Yu , Zhang Weini , Jiang Mingmei , Shen Xiaoyue , Wu Hailian , Chen Xinhua , Di Guilan 
Published: July 2024

In recent years, the abalone aquaculture industry has been threatened by the bacterial pathogens. The immune responses mechanisms underlying the phagocytosis of haemocytes remain unclear in Haliotis discus hannai. It is necessary to investigate the immune mechanism in response to these bacterial pathogens challenges. In this study, the phagocytic activities of haemocytes in H. discus hannai were examined by flow cytometry combined with electron microscopy and transcriptomic analyses. The results of Vibrio parahaemolyticus, Vibrio alginolyticus and Staphylococcus aureu challenge using electron microscopy showed a process during phagosome formation in haemocytes. The phagocytic rate (PP) of S. aureus was higher than the other five foreign particles, which was about 63%. The PP of Vibrio harveyi was about 43%, the PP peak of V. alginolyticus in haemocyte was 63.7% at 1.5 h. After V. parahaemolyticus and V. alginolyticus challenge, acid phosphatase, alkaline phosphatase, total superoxide dismutase, lysozyme, total antioxidant capacity, catalase, nitric oxide synthase and glutathione peroxidase activities in haemocytes were measured at different times, differentially expressed genes (DEGs) were identified by quantitative transcriptomic analysis. The identified DEGs after V. parahaemolyticus challenge included haemagglutinin/amebocyte aggregation factor-like, supervillin-like isoform X4, calmodulin-like and kyphoscoliosis peptidase-like; the identified DEGs after V. alginolyticus challenge included interleukin-6 receptor subunit beta-like, protein turtle homolog B-like, rho GTPase-activating protein 6-like isoform X2, leukocyte surface antigen CD53-like, calponin-1-like, calmodulin-like, troponin C, troponin I-like isoform X4, troponin T-like isoform X18, tumor necrosis factor ligand superfamily member 10-like, rho-related protein racA-like and haemagglutinin/amebocyte aggregation factor-like. Some immune-related KEGG pathways were significantly up-regulated or down-regulated after challenge, including thyroid hormone synthesis, Th17 cell differentiation signalling pathway, focal adhesion, melanogenesis, leukocyte transendothelial migration, inflammatory mediator regulation of TRP channels, ras signalling pathway, rap1 signalling pathway. This study is the first step towards understanding the H. discus hannai immune system by adapting several tools to gastropods and providing a first detailed morpho-functional study of their haemocytes.
Erythrocytes of the common carp are immune sentinels that sense pathogen molecular patterns, engulf particles and secrete pro-inflammatory cytokines against bacterial infection
Authors: Majstorović Jovana , Kyslík Jiří , Klak Katarzyna , Maciuszek Magdalena , Chan Justin T. H. , Korytář Tomáš , Holzer Astrid S. 
Published: July 2024

IntroductionRed blood cells (RBCs), also known as erythrocytes, are underestimated in their role in the immune system. In mammals, erythrocytes undergo maturation that involves the loss of nuclei, resulting in limited transcription and protein synthesis capabilities. However, the nucleated nature of non-mammalian RBCs is challenging this conventional understanding of RBCs. Notably, in bony fishes, research indicates that RBCs are not only susceptible to pathogen attacks but express immune receptors and effector molecules. However, given the abundance of RBCs and their interaction with every physiological system, we postulate that they act in surveillance as sentinels, rapid responders, and messengers.MethodsWe performed a series of in vitro experiments with Cyprinus carpio RBCs exposed to Aeromonas hydrophila, as well as in vivo laboratory infections using different concentrations of bacteria.ResultsqPCR revealed that RBCs express genes of several inflammatory cytokines. Using cyprinid-specific antibodies, we confirmed that RBCs secreted tumor necrosis factor alpha (TNFα) and interferon gamma (IFNγ). In contrast to these indirect immune mechanisms, we observed that RBCs produce reactive oxygen species and, through transmission electron and confocal microscopy, that RBCs can engulf particles. Finally, RBCs expressed and upregulated several putative toll-like receptors, including tlr4 and tlr9, in response to A. hydrophila infection in vivo.DiscussionOverall, the RBC repertoire of pattern recognition receptors, their secretion of effector molecules, and their swift response make them immune sentinels capable of rapidly detecting and signaling the presence of foreign pathogens. By studying the interaction between a bacterium and erythrocytes, we provide novel insights into how the latter may contribute to overall innate and adaptive immune responses of teleost fishes.
The impact of non-lethal doses of pyriproxyfen on male and female Aedes albopictus reproductive fitness
Authors: Kancharlapalli Sri Jyosthsna , Brelsfoard Corey L. 
Published: July 2024

IntroductionControl of the mosquito Aedes albopictus is confounded by its behavior due to females preferring to oviposition in small natural and artificial containers that are often difficult to remove or treat with insecticides. Autodissemination strategies utilizing highly potent insect growth regulators (IGRs) have emerged as promising tools for the control of this container-inhabiting species. The intended goal of autodissemination approaches is to use mosquitoes to self-deliver an IGR to these cryptic oviposition locations. Previous studies have focused on the efficacy of these approaches to impact natural populations, but little focus has been placed on the impacts on mosquitoes when exposed to non-lethal doses of IGRs similar to the levels they would be exposed to with autodissemination approaches.MethodsIn this study, the impact of non-lethal doses of pyriproxyfen (PPF) on the reproductive fitness of Ae. albopictus was investigated. Female and male Ae. albopictus mosquitoes were exposed to non-lethal doses of PPF and their fecundity and fertility were measured. To examine the impact of non-lethal doses of PPF, the expression of the ecdysone-regulated genes USP, HR3, and Vg, which are involved in vitellogenesis, was determined.ResultsOur results demonstrated a significant reduction in female fecundity and in the blood feeding and egg hatching rates upon exposure to non-lethal doses of PPF. Oocyte development was also delayed in PPF-treated females. Furthermore, exposure to non-lethal doses of PPF altered the expression of the genes involved in vitellogenesis, indicating disruption of hormonal regulation. Interestingly, PPF exposure also reduced the sperm production in males, suggesting a potential semi-sterilization effect.DiscussionThese findings suggest that non-lethal doses of PPF could enhance the efficacy of autodissemination approaches by impacting the reproductive fitness of both males and females. However, further research is needed to validate these laboratory findings in field settings and to assess their practical implications for vector control strategies.