bluuebunny/crossref_metadata_2025_split huggingface dataset:
A sperm-enriched 5’fragment of tRNA-Valine regulates preimplantation embryonic transcriptome and development
Authors: Liu Simeiyun , Holmes Andrew D. , Katzman Sol , Sharma Upasna 
Published: August 2024

ABSTRACTSperm small RNAs have been implicated in intergenerational epigenetic inheritance of paternal environmental effects; however, their biogenesis and functions remain poorly understood. We previously identified a 5’ fragment of tRNA-Valine-CAC-2 (tRFValCAC) as one of the most abundant small RNA in mature sperm. tRFValCAC is specifically enriched in sperm during post-testicular maturation in the epididymis, and we found that it is delivered to sperm from epididymis epithelial cells via extracellular vesicles. Here, we investigated the mechanistic basis of tRFValCAC delivery to sperm and its functions in the early embryo. We show that tRFValCAC interacts with an RNA binding protein, heterogeneous nuclear ribonucleoprotein A/B (hnRNPAB), in the epididymis, and this interaction regulates the sorting and packing of tRFValCAC into extracellular vesicles. In the embryo, we found that tRFValCAC regulates early embryonic mRNA processing and splicing. Inhibition of tRFValCAC in preimplantation embryos altered the transcript abundance of genes involved in RNA splicing and mRNA processing. Importantly, tRFValCAC-inhibited embryos showed altered mRNA splicing, including alternative splicing of various splicing factors and genes important for proper preimplantation embryonic development. Finally, we find that inhibition of tRFValCAC in zygotes delayed preimplantation embryonic development. Together, our results reveal a novel function of a sperm-enriched tRF in regulating alternating splicing and preimplantation embryonic development and shed light on the mechanism of sperm small RNA-mediated epigenetic inheritance.
Msh2-Msh3 DNA-binding is not sufficient to promote trinucleotide repeat expansions inSaccharomyces cerevisiae
Authors: Casazza Katherine M. , Williams Gregory M. , Johengen Lauren , Twoey Gavin , Surtees Jennifer A. 
Published: August 2024

AbstractMismatch repair (MMR) is a highly conserved DNA repair pathway that recognizes mispairs that occur spontaneously during DNA replication and coordinates their repair. InSaccharomyces cerevisiae, Msh2-Msh3 and Msh2-Msh6 initiate MMR by recognizing and binding insertion deletion loops (in/dels) up to ∼ 17 nucleotides (nt.) and base-base mispairs, respectively; the two complexes have overlapping specificity for small (1-2 nt.) in/dels. The DNA-binding specificity for the two complexes resides in their respective mispair binding domains (MBDs) and have distinct DNA-binding modes. Msh2-Msh3 also plays a role in promotingCAG/CTGtrinucleotide repeat (TNR) expansions, which underlie many neurodegenerative diseases such as Huntington’s Disease and Myotonic Dystrophy Type 1. Models for Msh2-Msh3’s role in promoting TNR tracts expansion have invoked its specific DNA-binding activity and predict that the TNR structure alters its DNA binding and downstream activities to block repair. Using a chimeric Msh complex that replaces the MBD of Msh6 with the Msh3 MBD, we demonstrate that Msh2-Msh3 DNA-binding activity is not sufficient to promote TNR expansions. We propose a model for Msh2-Msh3-mediated TNR expansions that requires a fully functional Msh2-Msh3 including DNA binding, coordinated ATP binding and hydrolysis activities and interactions with Mlh complexes that are analogous to those required for MMR.Article SummaryThe mismatch repair (MMR) protein complex Msh2-Msh3 promotes trinucleotide repeat (TNR) expansions that can lead to neurodegenerative diseases, while the Msh2-Msh6 complex does not. We tested the hypothesis that Msh2-Msh3’s specific DNA binding activity is sufficient to promote TNR expansions, using a chimeric MSH complexin vivoandin vitro. We found that the Msh2-Msh3-like DNA-binding was not sufficient to promote TNR expansions. Our findings indicate that Msh2-Msh3 plays an active, pathogenic role in promoting TNR expansions beyond simply binding to TNR structures.
The molecular structure of an axle-less F1-ATPase
Authors: Furlong Emily J. , Reininger-Chatzigiannakis Ian-Blaine P. , Zeng Yi C. , Brown Simon H. J. , Sobti Meghna , Stewart Alastair G. 
Published: August 2024

AbstractF1FoATP synthase is a molecular rotary motor that can generate ATP using a transmembrane proton motive force. Isolated F1-ATPase catalytic cores can hydrolyse ATP, passing through a series of conformational states involving rotation of the central γ rotor subunit and the opening and closing of the catalytic β subunits. Cooperativity in F1-ATPase has long thought to be conferred through the γ subunit, with three key interaction sites between the γ and β subunits being identified. Single molecule studies have demonstrated that the F1complexes lacking the γ axle still “rotate” and hydrolyse ATP, but with less efficiency. We solved the cryogenic electron microscopy structure of an axle-lessBacillussp. PS3 F1-ATPase. The unexpected binding-dwell conformation of the structure in combination with the observed lack of interactions between the axle-less γ and the open β subunit suggests that the complete γ subunit is important for coordinating efficient ATP binding of F1-ATPase.
The social determinants of suicide: an umbrella review
Authors: Kerrie Gallagher , Grace Phillips , Paul Corcoran , Stephen Platt , Heather McClelland , Michelle O’ Driscoll , Eve Griffin 
Published: August 2024

ABSTRACTPrevious research has highlighted the role of social determinants of health on mental health outcomes, but their impact on suicide mortality is less understood. The aim of this umbrella review was to systematically examine the association between 10 social determinants of health, as defined by the World Health Organization, and suicide mortality. A keyword search of titles and abstracts was conducted in six digital databases for studies published to 24 August 2023. Inclusion criteria were peer-reviewed systematic reviews and meta-analyses in English examining the association between these determinants and suicide. Methodological quality was assessed using an adapted AMSTAR-2 tool. Due to significant heterogeneity in the included studies, a meta-analysis was not undertaken. A narrative synthesis, structured by social determinant, was conducted. 49 records (25 meta-analyses and 24 systematic reviews) were eligible for inclusion in this review. The social determinants with the most available evidence were housing, basic amenities and the environment (n=21), income and social protection (n=13), unemployment (n=8) and early childhood development (n=6). Limited evidence was identified for education (n=3), social inclusion and non-discrimination (n=3) and working life conditions (n=3). No reviews examined the relationship between affordable healthcare services, structural conflict or food insecurity and suicide mortality. There was evidence of a modest effect of social determinants on suicide mortality. Most evidence related to unemployment, job insecurity, income and social protection and childhood adversity. The methodological quality of the included reviews varied considerably. High-quality research fully exploring the relationship between social and environmental factors and suicide risk is needed.
Hybrid MCDM approach for analyzing barriers and formulating strategies for the adoption of modular construction in developing countries
Authors: Oyefusi Oluwatobi Nurudeen , Arowoiya Victor Adetunji , Chan Melissa 
Published: August 2024

PurposeThe construction industry in developed countries is witnessing a paradigm shift towards modular construction methods, driven by the need for efficiency, sustainability, and cost-effectiveness. However, the realization of these benefits in the context of developing countries is hindered by numerous barriers. Against this backdrop, this study seeks to contribute insights into the barriers hindering the adoption of modular construction in developing countries, specifically Nigeria, and further formulate effective strategies.Design/methodology/approachA thorough review of existing literature was conducted to identify the multifaceted barriers hindering the adoption of modular construction and the corresponding strategies. Subsequently, a panel of 13 experts were invited to utilize the Fuzzy Analytic Hierarchy Process (FAHP) approach to systematically evaluate these barriers based on their impact. Furthermore, the experts implored the Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) approach to select and prioritize the most suitable strategies to mitigate these barriers.FindingsThe study revealed that the most critical barriers to modular construction are Client resistance to change and innovation, Limited experience in module installation, and Transportation constraints. Additionally, the study prioritizes 13 strategies, with the Development of effective guidelines, standards, and policies ranked highest. The insights from the ranking using the FAHP and TOPSIS approach were adopted to develop a framework for modular implementation in developing countries.Research limitations/implicationsThis study is limited to Nigeria due to its status as the country with the highest Gross Domestic Product (GDP) in Africa, and it is considered a suitable representation of the region as most of the countries in Africa are categorized as developing nations.Practical implicationsBy highlighting the most critical barriers and prioritizing effective strategies, the study provides actionable insights for overcoming obstacles to modular construction adoption. Decision-makers can use this information to develop targeted policies and training programs to promote the adoption of modular construction in developing countries.Originality/valueThe research provides valuable insights by not only identifying critical barriers but also presenting prioritized strategies, distinguishing itself from previous studies, and establishing itself as a novel resource for developing countries. This adopt a novel hybrid MCDM approach for modular construction in developing countries such as Nigeria which can serve as reference point to other developing countries seeking to adopt modular construction and leverage its numerous benefits.
Enhancing bank efficiency through board diversity: a MENA region study
Authors: Issa Ayman , Sahyouni Ahmad , Mateev Miroslav 
Published: August 2024

PurposeThis empirical research investigates the impact of board nationality diversity on the efficiency of banks. Additionally, our analysis examines the interacting impact of women's representation on bank boards in the correlation between nationality diversity on board and bank efficiency.Design/methodology/approachThis research utilizes a dataset comprising banks operating in the MENA countries over an eight-year period. We apply diverse statistical methodologies, with Ordinary Least Squares (OLS) being the primary econometric analysis, alongside several robustness tests.FindingsThe research results offer important insights into the importance of board nationality diversity, as well as its interaction with the inclusion of women on boards. The findings indicate that having foreign directors on bank boards enhances efficiency. Furthermore, they suggest that increased women representation on boards improves the positive correlation between presence of foreign directors in boardrooms and efficiency of banks, thereby mitigating agency problems and enhancing governance practices.Practical implicationsThese results carry substantial implications for legislators across the MENA countries. Advocating for diversity policies within banks to encourage the inclusion of foreign directors on their boards could lead to efficiency enhancements. Furthermore, policymakers might explore the implementation of quotas or directives to bolster gender heterogeneity within board appointments, ultimately fostering improved bank efficiency and bolstering competitiveness within the region.Originality/valueThis study breaks new ground by investigating how board nationality diversity affects efficiency of banking sector in the MENA countries. It stands out for examining the moderating role of women representation on boards, offering novel insights into how these factors interact.
Understanding information security awareness: evidence from the public healthcare sector
Authors: Neri Martina , Benevento Elisabetta , Stefanini Alessandro , Aloini Davide , Niccolini Federico , Carducci Annalaura , Federigi Ileana , Dini Gianluca 
Published: August 2024

Purpose
Information security awareness (ISA) mainly refers to those aspects that need to be addressed to effectively respond to information security challenges. This research used focus groups to empirically investigate the main ISA dimensions that emerge from the Italian public health-care sector. This study aims to identify the most critical dimension of ISA and to evaluate the diffusion and maturity of information security policies (ISPs) of health-care infrastructure and training programs.

Design/methodology/approach
This research adopted a qualitative research design and focus groups as a research methodology. Data analysis was conducted using the NVIVO 14 software package and followed the principles of thematic analysis.

Findings
The focus group results highlighted that health-care personnel find it difficult to comply with the main ISA dimensions, a situation that leads to risky behaviors. Password management, data storage and transfer and instant messaging applications emerged as the most critical of the main ISA dimensions in the context of this research. It also transpired that ISPs are not all-encompassing as they mainly focus on privacy problems but neglect security concerns. Finally, training programs are not fully implemented in the investigated context, thus undermining their positive enhancing role for ISA.

Originality/value
The public health-care sector emerged as a critical yet still under-investigated context. The need for an in-depth investigation of organizational sciences approaches to overcoming information security challenges is also recommended in several prior research studies.
The role of high human capital and green economies in environmental sustainability in the Asia-Pacific region, 1990–2022
Authors: Soto Gonzalo Hernández 
Published: August 2024

PurposeConsidering the inherent relationship between environmental degradation and the process of economic development, the latter is particularly reliant on the accumulation of human capital, which also emerges as one of the fundamental principles underlying green growth. However, this relationship tends to overlook varying levels of human capital. Hence, the purpose of this study is to examine the enduring associations between the stock of high human capital and green economies in terms of environmental sustainability among the key countries in the Asia Pacific region, namely Australia, Japan, Singapore, and South Korea, spanning the period from 1990 to 2022.Design/methodology/approachThis paper employs second-generation techniques. The long-term relationships were estimated using two constantly updated models - fully modified and bias corrected, CUP-FM and CUP-BC, respectively, to guarantee the robustness of our conclusions for the presence of cross-sectional dependency.FindingsThere is a long-term relationship between the stock of high human capital and the sustainability of the environment, in the same way that we have also found the same relationship between the development of socioeconomic practices of green economies. Finally, we conclude that, in the same way as the environmental Kuznets curve, the countries in our sample incur less environmental pollution as their level of income increases. This relationship may be motivated by a process of technological substitution and investment in the development of new techniques and technology to improve the efficiency of productivity with respect to the environment.Practical implicationsWe suggest that investing in education and promoting green economies can be powerful tools in the fight against climate change and promoting environmental sustainability. By prioritizing investments in renewable energy and sustainable technologies, policymakers can promote long-term economic and environmental health. Moreover, the findings suggest that promoting education in countries with high levels of environmental pollution can develop the knowledge and skills needed to implement sustainable practices and technologies. Ultimately, these efforts can contribute to improving income, productivity, and society's living conditions while reducing the environmental impact.Originality/valueThis research studies for the first time the load capacity curve hypothesis in determining the effects of the stock of high human capital and green economies on the environment. Consequently, limited papers have used the load capacity factor in the study of the relationships that we propose, especially that of human capital, which has scarcely been studied in relation to its contribution to the environmental fight.
How enduring and multi-faceted self-disgust threatens psychological recovery from anorexia nervosa: a qualitative enquiry
Authors: Bell Katie , Coulthard Helen , Wildbur Diane , Williamson Iain 
Published: August 2024

Purpose
Self-disgust appears to be a prominent feature in anorexia nervosa (AN), which might help explain why AN is often such a persistent disorder. Little is known about how this emotion can impact on recovering from this disorder. This study aims to develop our understanding of how people experience the emotion of self-disgust after physical recovery from AN.

Design/methodology/approach
Twelve female participants who reported previously having had a clinical diagnosis of AN but had physically recovered according to their EDE-Q scores took part in a semi-structured interview to explore their experiences of recovery and the role self-disgust played within this. Interpretative phenomenological analysis was used to explore the data.

Findings
Three themes were identified within the data to explain the experiences of self-disgust in those with AN: continued self-disgust following physical “Recovery”, multiple manifestations of self-disgust in recovery and increasing self-disgust in recovery as a driver for relapse.

Practical implications
Self-disgust was something each participant appeared to experience often, despite being physically recovered from AN. Disgust-based reactions to the self are enduring and highly resistant to change even whilst other aspects of the disorder become less potent. Self-disgust is multi-faceted and may trigger relapse as the signs of improvement and behaviours inherent in recovering were generally viewed as disgusting to the individuals.

Originality/value
Self-disgust is an emotion that continues to affect people with AN despite physical recovery. The recovery process itself is not linear and self-disgust is enduring and may cause those affected to relapse. Considering this emotion within therapeutic intervention may encourage those with AN to accept their recovered self.
Social media communication, consumer attitude and purchase intention in lifestyle category products: a PLS-SEM modeling
Authors: Singh Sumanjeet , Chaubey Dhani Shanker , Raj Rohit , Kumar Vimal , Paliwal Minakshi , Mahlawat Seema 
Published: August 2024

PurposeThis study explores the intricate relationship between social media communication, consumer attitude and purchase intention within the context of lifestyle category products. With the rapid proliferation of social media platforms, businesses have turned to these platforms to connect with consumers and influence their purchasing decisions. This study aims to provide an in-depth analysis of how social media communication strategies impact consumer attitudes and, in turn, influence purchase intentions.Design/methodology/approachThe study employs partial least squares structural equation modeling (PLS-SEM) to analyze the data collected from a sample of consumers.FindingsThe results of this study present that lack of visibility (LV), low-efficiency levels (LEL) and unpredictable elements (UE) are ranked as the top three major risk hurdles whereas real-time information on a package’s location (LV1), putting a GPS tracking system to track last-mile journey (OT3) and users wants on time location of their package (LV2) are ranked as top three most significant criteria affecting the practices of modern last-mile logistics in e-commerce businesses.Research limitations/implicationsThe results of this study contribute to our understanding of how social media influences consumer behavior in the lifestyle product sector, shedding light on the underlying mechanisms that drive consumer purchasing decisions.Originality/valueBy constructing and testing experimentally a research model that reveals a thorough analysis of pertinent literature and identifies multiple important elements influencing consumer behavior in the lifestyle category, this paper adds to the body of knowledge on marketing. Practical ramifications for lifestyle firms are examined, along with suggestions for improving their social media tactics, in light of the findings.
Recognition and pose estimation method for random bin picking considering incomplete point cloud scene
Authors: Kan Yan , Li Hao , Chen Zhengtao , Sun Changjiang , Wang Hao , Seidelmann Joachim 
Published: August 2024

Purpose
This paper aims to propose a stable and precise recognition and pose estimation method to deal with the difficulties that industrial parts often present, such as incomplete point cloud data due to surface reflections, lack of color texture features and limited availability of effective three-dimensional geometric information. These challenges lead to less-than-ideal performance of existing object recognition and pose estimation methods based on two-dimensional images or three-dimensional point cloud features.

Design/methodology/approach
In this paper, an image-guided depth map completion method is proposed to improve the algorithm's adaptability to noise and incomplete point cloud scenes. Furthermore, this paper also proposes a pose estimation method based on contour feature matching.

Findings
Through experimental testing on real-world and virtual scene dataset, it has been verified that the image-guided depth map completion method exhibits higher accuracy in estimating depth values for depth map hole pixels. The pose estimation method proposed in this paper was applied to conduct pose estimation experiments on various parts. The average recognition accuracy in real-world scenes was 88.17%, whereas in virtual scenes, the average recognition accuracy reached 95%.

Originality/value
The proposed recognition and pose estimation method can stably and precisely deal with the difficulties that industrial parts present and improve the algorithm's adaptability to noise and incomplete point cloud scenes.