bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced peroxisomal citrate synthase activity increases substrate availability for polyhydroxyalkanoate biosynthesis in plant peroxisomes
Authors: Tilbrook Kimberley , Poirier Yves , Gebbie Leigh , Schenk Peer M. , McQualter Richard B. , Brumbley Stevens M. 
Published: June 2014

SummaryPolyhydroxyalkanoates (PHAs) are bacterial carbon storage polymers used as renewable, biodegradable plastics. PHA production in plants may be a way to reduce industrial PHA production costs. We recently demonstrated a promising level of peroxisomal PHA production in the high biomass crop species sugarcane. However, further production strategies are needed to boost PHA accumulation closer to commercial targets. Through exogenous fatty acid feeding of Arabidopsis thaliana plants that contain peroxisome‐targeted PhaA, PhaB and PhaC enzymes from Cupriavidus necator, we show here that the availability of substrates derived from the β‐oxidation cycle limits peroxisomal polyhydroxybutyrate (PHB) biosynthesis. Knockdown of peroxisomal citrate synthase activity using artificial microRNA increased PHB production levels approximately threefold. This work demonstrates that reduction of peroxisomal citrate synthase activity may be a valid metabolic engineering strategy for increasing PHA production in other plant species.
Differences in MAT gene distribution and expression between Rhynchosporium species on grasses
Authors: King K. M. , West J. S. , Fitt B. D. L. , Dyer P. S. 
Published: June 2014

Leaf blotch is a globally important disease of barley crops and other grasses that is caused by at least five host‐specialized species in the fungal genus Rhynchosporium. The pathogen R. commune (specialized to barley, brome‐grass and Italian ryegrass) has long been considered to reproduce only by asexual means, but there has been accumulating evidence for recombination and gene flow from population genetic studies and the detection of complementary MAT1‐1 and MAT1‐2 isolates in a c. 1:1 ratio in the field. Here, it is demonstrated that 28 isolates of the closely related species R. agropyri (on couch‐grass) and R. secalis (on rye and triticale), collected from Europe, were also either of MAT1‐1 or MAT1‐2 genotype and that the distribution of mating types did not deviate significantly from a 1:1 ratio. Evidence is then provided for MAT1‐1‐1 and MAT1‐2‐1 gene expression during mycelial growth for all three species. By contrast, 27 isolates of the more distantly related R. orthosporum (on cocksfoot) and R. lolii (on Italian and perennial ryegrasses) from Europe were exclusively of the MAT1‐1 genotype, and expression of the MAT1‐1‐1 gene could not be detected during mycelial growth. These data suggest that cryptic sexual cycles are more likely to exist for R. commune, R. agropyri and R. secalis than for either R. orthosporum or R. lolii. A phylogenetic analysis of partial MAT1‐1 idiomorph sequences resolved these five species into two distinct groups (R. commune, R. agropyri and R. secalis versus R. orthosporum and R. lolii) but provided only limited resolution within each group.
Emotional content modulates response inhibition and perceptual processing
Authors: Yang Suyong , Luo Wenbo , Zhu Xiangru , Broster Lucas S. , Chen Taolin , Li Jinzhen , Luo Yuejia 
Published: June 2014

AbstractIn this study, event‐related potentials were used to investigate the effect of emotion on response inhibition. Participants performed an emotional go/no‐go task that required responses to human faces associated with a “go” valence (i.e., emotional, neutral) and response inhibition to human faces associated with a “no‐go” valence. Emotional content impaired response inhibition, as evidenced by decreased response accuracy and N2 amplitudes in no‐go trials. More importantly, emotional expressions elicited larger N170 amplitudes than neutral expressions, and this effect was larger in no‐go than in go trials, indicating that the perceptual processing of emotional expression had priority in inhibitory trials. In no‐go trials, correlation analysis showed that increased N170 amplitudes were associated with decreased N2 amplitudes. Taken together, our findings suggest that emotional content impairs response inhibition due to the prioritization of emotional content processing.
Mild Renal Artery Stenosis Can Induce Renovascular Hypertension and is Associated with Elevated Renal Vein Renin Secretion
Authors: Balamuthusamy Saravanan , Kannan Arun , Thajudeen Bijin , Ottley Dean , Jalandhara Nishant 
Published: June 2014

AbstractRenovascular hypertension is a syndrome which encompasses the physiological response of the kidney to changes in renal blood flow and renal perfusion pressure. Such physiological changes can occur with renal artery occlusion irrespective of the severity of the lesion. We have analyzed hypertensive patients with mild renal artery stenosis and compared them to patients with no stenosis. Renal vein renin sampling from catheterization of the renal vein was performed in all these patients. Patients with mild stenosis had higher renal vein renin ratio (3.01 ± 1.5) than the patients with no stenosis (1.10 ± 0.29; p = 0.002). Patients with mild stenosis were also found to have higher diastolic blood pressure and renal artery resistive indices when compared to patients with no stenosis. We therefore conclude that mild stenosis can precipitate renin‐mediated hypertension in renovascular stenosis and also emphasis that parameters pertinent to renal physiology need to be evaluated before considering treatment options in patients with renal artery stenosis and medical management with RAAS blockade is the preferred modality of therapy for patients with renin‐mediated hypertension.
Globalization and the Question of Social Justice
Authors: Agartan Kaan 
Published: June 2014

AbstractPrevious conceptions of social justice presupposed a closed political community in which nation‐states were to be in full control of policy instruments that reinforced mechanisms of social justice. States’ governing capacity to deliver social justice to their citizens has been challenged in the face of deepening transnational interactions and interdependencies in economic, political, and cultural realms, as these interactions and interdependencies directly affect the lives of millions of people. This paper revisits an ongoing debate on Global Social Justice and aims to introduce two clashing views – namely, Minimalist and Cosmopolitan approaches – on whether or not people in affluent societies have distinctive duties of concern for people in less developed countries. After outlining the main contours of the arguments on both sides, the paper concludes by suggesting that the debate can inform different areas of sociological inquiry that are directly related to issues such as power, inequality, and social exclusion.
Capturing Capital to Negotiate the Intersections of Motherhood and Work
Authors: Brooks Jane E. , Rogalin Christabel L. 
Published: June 2014

AbstractWomen are judged and judge themselves in response to cultural norms about motherhood and employment. While much has been written in sociology and other disciplines about the intersections of motherhood and work, there is not much on their ideals and the enactment of the ideals of womanhood and worker in connection to various forms of capital. In this paper, we review the literature on motherhood to explore the ways in which these ideals are related to the meanings of mother and worker. Further, we highlight how differential access to economic and non‐economic capital due to one's social location influences a woman's ability to be viewed successful simultaneously as mother and worker.
Residue incorporation mitigates tillage‐induced loss of soil carbon in laboratory microcosms
Authors: Bajgai Y. , Hulugalle N. , Kristiansen P. , McHenry M. , Cowie A. 
Published: June 2014

AbstractAnnual horticultural systems rely on frequent and intensive tillage to prepare beds, manage weeds and control insects. But this practice reduces soil organic carbon (SOC) through accelerated CO2 emission. Crop residue incorporation could counteract this loss. We investigated whether vegetable systems could be made more resilient by including a high‐residue grain crop such as sweet corn (Zea mays L. var. rugosa), in the rotation through the use of conventional (no residue, no soil sieving) and organic (residue incorporated and soil sieved) soil management scenarios. We evaluated short‐term emission of CO2‐C and soil C content in incubated Chromosol and Vertosol soils (Australian Classification) with and without sieving (simulated tillage) or the incorporation of ground sweet corn residue. Residue treatment emitted 2.3 times more CO2‐C compared to the no‐residue treatment, and furthermore, sieved soil emitted 1.5 times more CO2‐C than the unsieved across the two soil types. The residue incorporation had a greater effect on CO2‐C flux than simulated tillage, suggesting that C availability and form can be more important than physical disturbance in cropping soils. The organic scenario (with residue and sieved) emitted more CO2‐C, but had 13% more SOC compared with the conventional scenario (without residue and unsieved), indicating that organic systems may retain more SOC than a conventional system. The SOC lost by soil disturbance was more than offset by the incorporation of residue in the laboratory conditions. Therefore, the possible SOC loss by tillage for weed control under organic management may be offset by organic residue input.
Sur les rapprochements par conjugaison en dimension 1 et classe
Authors: Navas Andrés 
Published: June 2014

AbstractWe show that the space of actions of every finitely generated, nilpotent group by $C^1$ orientation-preserving diffeomorphisms of the circle is path-connected. This is done via a general result that allows any given action on the interval to be connected to the trivial one by a continuous path of topological conjugates.
Single Versus Combined Cognitive and Physical Activity Effects on Fluid Cognitive Abilities of Healthy Older Adults: A 4-Month Randomized Controlled Trial With Follow-Up
Authors: Linde Katja , Alfermann Dorothee 
Published: June 2014

Background:Physical and cognitive activity seems to be an effective strategy by which to promote age-sensitive fluid cognitive abilities in older adults.Method:In this randomized controlled trial, 70 healthy senior citizens (age 60–75) were allocated to a physical, cognitive, combined physical plus cognitive, and waiting control group. The trial assessed information processing speed, short-term memory, spatial relations, concentration, reasoning, and cognitive speed.Results:In contrast to the control group, the physical, cognitive, and combined training groups enhanced their concentration immediately after intervention. Only the physical training group showed improved concentration 3 months later. The combined training group displayed improved cognitive speed both immediately and three months after intervention. The cognitive training group displayed improved cognitive speed 3 months after intervention.Conclusions:Physical, cognitive, and combined physical plus cognitive activity can be seen as cognition-enrichment behaviors in healthy older adults that show different rather than equal intervention effects.
Evaluation of Bio-Rad Geenius HIV-1 and -2 Assay as a Confirmatory Assay for Detection of HIV-1 and -2 Antibodies
Authors: Hawthorne Hallen Anne , Samuelson Agneta , Nordin Margareta , Albert Jan , Bogdanovic Gordana 
Published: June 2014

ABSTRACTThe performance of the new Bio-Rad Geenius HIV-1/2 confirmatory assay was compared with that of the Chiron RIBA HIV-1/HIV-2 strip immunoblot assay using 166 samples from HIV-1-, HIV-2-, or HIV-1/2-positive and HIV-negative individuals and two quality control panels. Our results indicate that the Geenius assay is a suitable alternative for confirmatory HIV testing.
Murine Cytomegalovirus Virion-Associated Protein M45 Mediates Rapid NF-κB Activation after Infection
Authors: Krause Eva , de Graaf Miranda , Fliss Patricia M. , Dölken Lars , Brune Wolfram 
Published: June 2014

ABSTRACTMurine cytomegalovirus (MCMV) rapidly induces activation of nuclear factor κB (NF-κB) upon infection of host cells. After a transient phase of activation, the MCMV M45 protein blocks all canonical NF-κB-activating pathways by inducing the degradation of the gamma subunit of the inhibitor of κB kinase complex (IKKγ; commonly referred to as the NF-κB essential modulator [NEMO]). Here we show that the viral M45 protein also mediates rapid NF-κB activation immediately after infection. MCMV mutants lacking M45 or expressing C-terminally truncated M45 proteins induced neither NF-κB activation nor transcription of NF-κB-dependent genes within the first 3 h of infection. Rapid NF-κB activation was absent in MCMV-infected NEMO-deficient fibroblasts, indicating that activation occurs at or upstream of the IKK complex. NF-κB activation was strongly reduced in murine fibroblasts lacking receptor-interacting protein 1 (RIP1), a known M45-interacting protein, but was restored upon complementation with murine RIP1. However, the ability of M45 to interact with RIP1 and NEMO was not sufficient to induce NF-κB activation upon infection. In addition, incorporation of the M45 protein into virions was required. This was dependent on a C-terminal region of M45, which is not required for interaction with RIP1 and NEMO. We propose a model in which M45 delivered by viral particles activates NF-κB, presumably involving an interaction with RIP1 and NEMO. Later in infection, expression of M45 induces the degradation of NEMO and the shutdown of canonical NF-κB activation.IMPORTANCETranscription factor NF-κB is an important regulator of innate and adaptive immunity. Its activation can be beneficial or detrimental for viral pathogens. Therefore, many viruses interfere with NF-κB signaling by stimulating or inhibiting the activation of this transcription factor. Cytomegaloviruses, opportunistic pathogens that cause lifelong infections in their hosts, activate NF-κB rapidly and transiently upon infection but block NF-κB signaling soon thereafter. Here we report the surprising finding that the murine cytomegalovirus protein M45, a component of viral particles, plays a dual role in NF-κB signaling. It not only blocks NF-κB signaling later in infection but also triggers the rapid activation of NF-κB immediately following virus entry into host cells. Both activation and inhibition involve M45 interaction with the cellular signaling mediators RIP1 and NEMO. Similar dual functions in NF-κB signaling are likely to be found in other viral proteins.
Triggering TLR2, -3, -4, -5, and -8 Reinforces the Restrictive Nature of M1- and M2-Polarized Macrophages to HIV
Authors: Schlaepfer Erika , Rochat Mary-Aude , Duo Li , Speck Roberto F. 
Published: June 2014

ABSTRACT

Macrophages must react to a large number of pathogens and their effects. In chronic HIV infection, the microenvironment changes with an influx of microbial products that trigger Toll-like receptors (TLRs). That dynamic nature can be replicated
ex vivo
by the proinflammatory (M1-polarized) and alternatively activated (M2-polarized) macrophages. Thus, we determined how polarized macrophages primed by various TLR agonists support HIV replication. Triggering of TLR2, -3, -4, -5, and -8 reinforced the low level of permissiveness in polarized macrophages. HIV was inhibited even more in M1-polarized macrophages than in macrophages activated only by TLR agonists. HIV was inhibited before its integration into the host chromosome. Polarization and triggering by various TLR agonists resulted in distinct cytokine profiles, endocytic activity, and distinct upregulation of restriction factors of HIV. Thus, different mechanisms likely contribute to the HIV-inhibitory effects. In chronic HIV infection, macrophages might become less permissive to HIV due to changes in the microenvironment. The high level of reactivity of polarized macrophages to TLR triggering may be exploited for immunotherapeutic strategies.

IMPORTANCE
Macrophages are a major target of HIV-1 infection. Different cell types in this very heterogeneous cell population respond differently to stimuli.
In vitro
, the heterogeneity is mimicked by their polarization into proinflammatory and alternatively activated macrophages. Here we explored the extent to which agonists triggering the TLR family affect HIV replication in polarized macrophages. We found that a number of TLR agonists blocked HIV replication substantially when given before infection. We also report the mechanisms of how TLR agonists exert their inhibitory action. Our findings may advance our understanding of which and how TLR agonists block HIV infection in polarized macrophages and may facilitate the design of novel immunotherapeutic approaches.
Dendritic Cells Restore CD8+T Cell Reactivity to Autologous HIV-1
Authors: Smith Kellie N. , Mailliard Robbie B. , Larsen Brendan B. , Wong Kim , Gupta Phalguni , Mullins James I. , Rinaldo Charles R. 
Published: June 2014

ABSTRACTRecall T cell responses to HIV-1 antigens are used as a surrogate for endogenous cellular immune responses generated during infection. Current methods of identifying antigen-specific T cell reactivity in HIV-1 infection use bulk peripheral blood mononuclear cells (PBMC) yet ignore professional antigen-presenting cells (APC) that could reveal otherwise hidden responses. In the present study, peptides representing autologous variants of major histocompatibility complex (MHC) class I-restricted epitopes from HIV-1 Gag and Env were used as antigens in gamma interferon (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) and polyfunctional cytokine assays. Here we show that dendritic cells (DC) enhanced T cell reactivity at all stages of disease progression but specifically restored T cell reactivity after combination antiretroviral therapy (cART) to early infection levels. Type 1 cytokine secretion was also enhanced by DC and was most apparent late post-cART. We additionally show that DC reveal polyfunctional T cell responses after many years of treatment, when potential immunotherapies would be implemented. These data underscore the potential efficacy of DC immunotherapy that aims to awaken a dormant, autologous, HIV-1-specific CD8+T cell response.IMPORTANCEAssessment of endogenous HIV-1-specific T cell responses is critical for generating immunotherapies for subjects on cART. Current assays ignore the ability of dendritic cells to reveal these responses and may therefore underestimate the breadth and magnitude of T cell reactivity. As DC do not prime new responses in these assays, it can be assumed that the observed responses are not detected without appropriate stimulation. This is important because dogma states that HIV-1 mutates to evade host recognition and that CD8+cytotoxic T lymphocyte (CTL) failure is due to the inability of T cells to recognize the autologous virus. The results presented here indicate that responses to autologous virus are generated during infection but may need additional stimulation to be effective. Detecting the breadth and magnitude of HIV-1-specific T cell reactivity generatedin vivois of the utmost importance for generating effective DC immunotherapies.
Intercontinental Dispersal of HIV-1 Subtype B Associated with Transmission among Men Who Have Sex with Men in Japan
Authors: Takebe Yutaka , Naito Yuki , Raghwani Jayna , Fearnhill Esther , Sano Takako , Kusagawa Shigeru , Mbisa Jean L. , Zhang Hongyi , Matano Tetsuro , Leigh Brown Andrew J. , Pybus Oliver G. , Dunn David , Kondo Makiko 
Published: June 2014

ABSTRACT

Transmission clusters of HIV-1 subtype B uniquely associated with the epidemic among men who have sex with men (MSM) in East Asia have recently been identified. Using the Los Alamos HIV sequence database and the UK HIV drug resistance database, we explored possible links between HIV MSM epidemics in East Asia and the rest of the world by using phylogenetic and molecular clock analyses. We found that JP.MSM.B-1, a subtype B MSM variant that accounts for approximately one-third of the infections among Japanese MSM, was detected worldwide, in the United Kingdom (
n
= 13), mainland China (
n
= 3), the United States, Germany, Canada, and Taiwan (
n
= 1 each). Interestingly, 10 United Kingdom samples plus two from Germany and the United States formed a distinct monophyletic subgroup within JP.MSM.B-1. The estimated divergence times of JP.MSM.B-1 and the latter subgroup were ∼1989 and ∼1999, respectively. These dates suggest that JP.MSM.B-1 was circulating for many years in Japan among MSM before disseminating to other countries, most likely through global MSM networks. A significant number of other Asian MSM HIV lineages were also detected in the UK HIV drug resistance database. Our study provides insight into the regional and global dispersal of Asian MSM HIV lineages. Further study of these strains is warranted to elucidate viral migration and the interrelationship of HIV epidemics on a global scale.

IMPORTANCE
We previously identified several transmission clusters of HIV-1 subtype B uniquely associated with the epidemic among men who have sex with men (MSM) in East Asia. Using the Los Alamos HIV sequence database and the UK HIV drug resistance database, we explored the possible interplay of HIV MSM epidemics in the different geographic regions and found previously unrecognized interrelationships among the HIV-1 epidemics in East Asia, the United Kingdom, and the rest of the world. Our study provides insight into the regional and global dispersal of Asian MSM HIV lineages and highlights the importance of strengthening HIV monitoring efforts and the need for implementing effective control measures to reduce HIV transmission on a global scale.