bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of fed-batch strategy to fully eliminate the negative effect of lignocellulose-derived inhibitors in ABE fermentation
Authors: Branska Barbora , Koppova Kamila , Husakova Marketa , Patakova Petra 
Published: June 2024

Abstract
Background
Inhibitors that are released from lignocellulose biomass during its treatment represent one of the major bottlenecks hindering its massive utilization in the biotechnological production of chemicals. This study demonstrates that negative effect of inhibitors can be mitigated by proper feeding strategy. Both, crude undetoxified lignocellulose hydrolysate and complex medium supplemented with corresponding inhibitors were tested in acetone–butanol–ethanol (ABE) fermentation using Clostridium beijerinckii NRRL B-598 as the producer strain.

Results
First, it was found that the sensitivity of C. beijerinckii to inhibitors varied with different growth stages, being the most significant during the early acidogenic phase and less pronounced during late acidogenesis and early solventogenesis. Thus, a fed-batch regime with three feeding schemes was tested for toxic hydrolysate (no growth in batch mode was observed). The best results were obtained when the feeding of an otherwise toxic hydrolysate was initiated close to the metabolic switch, resulting in stable and high ABE production. Complete utilization of glucose, and up to 88% of xylose, were obtained. The most abundant inhibitors present in the alkaline wheat straw hydrolysate were ferulic and coumaric acids; both phenolic acids were efficiently detoxified by the intrinsic metabolic activity of clostridia during the early stages of cultivation as well as during the feeding period, thus preventing their accumulation. Finally, the best feeding strategy was verified using a TYA culture medium supplemented with both inhibitors, resulting in 500% increase in butanol titer over control batch cultivation in which inhibitors were added prior to inoculation.

Conclusion
Properly timed sequential feeding effectively prevented acid-crash and enabled utilization of otherwise toxic substrate. This study unequivocally demonstrates that an appropriate biotechnological process control strategy can fully eliminate the negative effects of lignocellulose-derived inhibitors.

Graphical Abstract
Hard ticks (Ixodida: Ixodidae) in the Colombian Caribbean harbor the Jingmen tick virus: an emerging arbovirus of public health concern
Authors: López Yesica , Thomas Richard , Muñoz-Leal Sebastián , López-Mejia Yeimi , Galeano Ketty , Garcia Alejandra , Romero Luis , la Hoz Daniel Echeverri-De , Martinez Caty , Calderón Alfonso , Gastelbondo Bertha , Contreras Héctor , Olivieri Gino , Rubiano Luis , Paternina Luis , Hoyos-López Richard , Ortiz Anggie , Garay Evelyn , Alemán-Santos Maira , Rivero Ricardo , Miranda Jorge , Florez Luis , Ballesteros Jolaime , Contreras Verónica , Tique Vaneza , Fragoso Pedro , Guzman Camilo , Arrieta German , Mattar Salim 
Published: June 2024

Abstract
Background
Ticks are obligate hematophagous ectoparasites involved in transmitting viruses of public health importance. The objective of this work was to identify the Jingmen tick virus in hard ticks from the Colombian Caribbean, an arbovirus of importance for public health.

Methods
Ticks were collected in rural areas of Córdoba and Cesar, Colombia. Taxonomic identification of ticks was carried out, and pools of 13 individuals were formed. RNA extraction was performed. Library preparation was performed with the MGIEasy kit, and next-generation sequencing (NGS) with MGI equipment. Bioinformatic analyses and taxonomic assignments were performed using the Galaxy platform, and phylogenetic analyses were done using IQ-TREE2.

Results
A total of 766 ticks were collected, of which 87.33% (669/766) were Rhipicephalus microplus, 5.4% (42/766) Dermacentor nitens, 4.2% (32/766) Rhipicephalus linnaei, and 3.0% (23/766) Amblyomma dissimile. Complete and partial segments 1, 2, 3, and 4 of Jingmen tick virus (JMTV) were detected in the metatranscriptome of the species R. microplus, D. nitens, and A. dissimile. The JMTVs detected are phylogenetically related to JMTVs detected in Aedes albopictus in France, JMTVs detected in R. microplus in Trinidad and Tobago, JMTVs in R. microplus and A. variegatum in the French Antilles, and JMTVs detected in R. microplus in Colombia. Interestingly, our sequences clustered closely with JMTV detected in humans from Kosovo.

Conclusions
JMTV was detected in R. microplus, D. nitens, and A. dissimile. JMTV could pose a risk to humans. Therefore, it is vital to establish epidemiological surveillance measures to better understand the possible role of JMTV in tropical diseases.

Graphical Abstract
RETRACTED ARTICLE: Emerging insights into the role of IL-1 inhibitors and colchicine for inflammation control in type 2 diabetes
Authors: Guan Jianbin , Abudouaini Haimiti , Lin Kaiyuan , Yang Kaitan 
Published: June 2024

Abstract
Type 2 diabetes mellitus (T2DM), a prevalent chronic metabolic disorder, is closely linked to persistent low-grade inflammation, significantly contributing to its development and progression. This review provides a comprehensive examination of the inflammatory mechanisms underlying T2DM, focusing on the role of the NLRP3 inflammasome and interleukin-1β (IL-1β) in mediating inflammatory responses. We discuss the therapeutic potential of IL-1 inhibitors and colchicine, highlighting their mechanisms in inhibiting the NLRP3 inflammasome and reducing IL-1β production. Recent studies indicate that these agents could effectively mitigate inflammation, offering promising avenues for the prevention and management of T2DM. By exploring the intricate connections between metabolic disturbances and chronic inflammation, this review underscores the need for novel anti-inflammatory strategies to address T2DM and its complications.
Chemical fingerprint analysis of fermented Morinda citrifolia L. (Noni) juice by UHPLC Q-TOF/MS combined with chemometric analysis
Authors: Kim Yoonjeong , Pyeon Jiye , Lee Jae-Yeon , Kim Eun-Min , La Im-Joung , Lee Ok-Hwan , Kim Keono , Sung Jeehye , Kim Younghwa 
Published: June 2024

AbstractMorinda citrifolia L. (Noni) has been widely used in traditional medicine in tropical zones and has become increasingly popular globally owing to its health benefits. Most noni fruits are consumed as juice, which is traditionally produced by the natural fermentation of noni fruits. In this study, the metabolic profiles of noni fruit juice (NJ1) and fermented noni fruit juices (NJ2 and NJ3) was compared. A total of 74, 83, and 91 compounds including anthraquinones, coumarins, flavonoids, phenolic acids, phenolics, terpenoids, and miscellaneous (acids, carbohydrates, vitamins, fatty acids, etc.) were tentatively identified from NJ1, NJ2, and NJ3 in both positive and negative electrospray ionization modes. The phenolic compound composition differed significantly between noni juice and fermented noni juice. The results of the unsupervised principal component analysis and hierarchical clustering analysis showed that the non-fermented juice group clustered with the fermented juice groups. Asperulosidic acid, isoasperulosidic acid, and rutin levels were higher in the NJ1 group than those in the NJ2 group. Deacetylasperulosidic acid and monotropein contents in NJ2 were higher than those in NJ1. Similarly, NJ1 had higher asperulosidic acid and isoasperulosidic acid than those in NJ3. The findings from this study have the potential to enhance the quality of fermented noni juice.
Associated factors of dietary diversity among schoolchildren in Plateau Central region of Burkina Faso: a cross-sectional study
Authors: Ouedraogo Daniel Somwaoga , Compaore Ella W. R. , Ouedraogo Ousmane , Dicko Mamoudou H. 
Published: June 2024

Abstract
Context
School-age is a dynamic period of growth and development, leading to good health and a productive adult life. Adequate dietary intake provides essential nutrients for growth, health and cognition. However, the practices of adequate nutrition is still not a matter of course for schoolchildren in many countries. The aim of this study was to identify associated factors of dietary diversity among students in public primary school in the Central Plateau Region.

Method
Multi-stage sampling was used to select schoolchildren. A semi-structured questionnaire was used to collect information’s of food consumption at home and at school using a 24-h dietary recall method. Binary logistic regression was used to identify variables associated with students' dietary diversity scores (DDS) with statistical significance at p < 0.05, after performing Chi-square test of independence to identify candidates variables at p < 0.25.

Results
The study involved 560 pupils aged 6 to 14 older, including 52.9% girls and 47.9% boys. Dietary diversity was divided into three classes: low (DDS ≤ 4), medium (DDS = 5) and high (DDS ≥ 6). Thus, 13.4% of students have a low DDS and average in 48.9%, versus 37.7% high. Students in Ganzourgou were twice as likely to have a low DDS (AOR = 2.01, 95% CI:1.00–4.04) compared to those in Oubritenga. Household drinking water source, pupil status and father's occupation were significantly associated with pupils' dietary intake.

Conclusion
Primary schoolchildren don’t have good dietary practices in the Plateau Central Region. Promoting dietary diversification in households and balanced meals in school canteens would be necessary to improve the DDS of schoolchildren.

Trial registration
Clinical Trial Number: 2022_33_/MS/MESRSI/CERS of 02/14/2022.
Computer-aided design and 3D printing for a stable construction of segmental bone defect model in Beagles: a short term observation
Authors: Cheng Kai , Zhu Haotian , Peng Yuanhao , Wen Xinghua , Ding Huanwen 
Published: June 2024

Abstract
Objective
Segmental bone defect animal studies require stable fixation which is a continuous experimental challenge. Large animal models are comparable to the human bone, but with obvious drawbacks of housing and costs. Our study aims to utilize CAD and 3D printing in the construction of a stable and reproducible segmental bone defect animal mode.

Methods
CAD-aided 3D printed surgical instruments were incorporated into the construction of the animal model through preoperative surgical emulation. 20 3D printed femurs were divided into either experimental group using 3D surgical instruments or control group. In Vitro surgical time and accuracy of fixation were analysed and compared between the two groups. A mature surgical plan using the surgical instruments was then utilized in the construction of 3 segmental bone defect Beagle models in vivo. The Beagles were postoperatively assessed through limb function and imaging at 1, 2 and 3 months postoperatively.

Results
In vitro experiments showed a significant reduction in surgical time from 40.6 ± 14.1 (23–68 min) to 26 ± 4.6 (19–36 min) (n = 10, p < 0.05) and the accuracy of intramedullary fixation placement increased from 71.6 ± 23.6 (33.3–100) % to 98.3 ± 5.37 (83–100) %, (n = 30, p < 0.05) with the use of CAD and 3D printed instruments. All Beagles were load-bearing within 1 week, and postoperative radiographs showed no evidence of implant failure.

Conclusion
Incorporation of CAD and 3D printing significantly increases stability, while reducing the surgical time in the construction of the animal model, significantly affecting the success of the segmental bone defect model in Beagles.
Sounding the alarm regarding mental health of children and adolescents in relation to parenting style
Authors: Mohamed Nesreen Mosbah Elsayed , Abd-Elmageed Fawzia Nabeel Mohammad , Ramadan Rasha Abdelateef Abdelaziz 
Published: June 2024

Abstract
Background
Childhood and adolescence are critical periods for physical and mental development. For that, sounding the alarm for the warning signs and red flags of children’s mental health disorders is important to promote good health and mental wellness throughout the lifespan. The aim of the study was to assess children’s and adolescents’ mental health in relation to parenting styles.

Methods
This study used a descriptive cross-sectional design. From early May 2022 until late October 2022, Zagazig University in Egypt hosted this investigation. Subjects: For this study, 400 parents of Zagazig University staff, employees, and workers who agreed to engage in the current study were gathered as a convenience sample. Tools: In order to get the required data, three tools were utilized. Tool I: A questionnaire for interviews to gather demographic information about the participating parents and their children. Tool II: Adapted Ontario Child Health Study Emotional Behavioural Scale: Parent Version (for children 4–17 years). Tool III: Parenting style scale.

Results
It was found that criteria for conduct disorder constituted the highest followed by criteria for attention deficit hyperactivity disorder and major depression disorder with a mean and standard deviation of 15.10 ± 3.7, 12.83 ± 3.4, and 11.9 ± 2.8. Also, 66% of the participating parents practiced a permissive parenting style while 18% of them practiced the authoritative style and 16% for the authoritarian style.

Conclusion
It was determined that criteria of conduct disorder were the most prevalent, followed by criteria of attention deficit hyperactivity disorder and major depressive disorder. Additionally, there was high statistical significance between mental health disorders, parenting styles, and parental educational level. In order to protect children’s and adolescents’ mental health, this study recommended alerting parents and teachers about red flags and warning signs of mental health disorders for early detection and management. Additionally, educate parents about effective parenting methods and how to behave correctly with their children. Psychological counseling centers for seeking help should be available everywhere and announced.
Word-in-noise perception test in children
Authors: Emami Seyede Faranak , Shariatpanahi Elnaz , Gohari Nasrin , Mehrabifard Mobina 
Published: June 2024

Abstract
Background
The word-in-noise discrimination test evaluates the phonological aspect of speech based on the detection of the vowels. While word-in-noise perception (WINP) test is one of the speech perception exams and evaluates the ability to understand the meaning of words by discovering the consonants.
Until now, all word-in-noise tests have assessed speech discrimination performance, and for the first time, the normal values of the WINP test for adults have been determined. Since the normal values of WINP scores in children have not been reported, our research aimed to determine the normal values of the WINP test in children aged 5 to 13 years.
In this cross-sectional study, 120 Persian speaking Iranain children with normal hearing thresholds were evaluated from the beginning to the end of spring 2023. We divided them into 4 age groups (A = 5–7 < years, B = 7–9 < years, C = 9–11 < years, D = 11–13 < years). The evaluations included general audiology tests and WINP tests using homtonic-monosyllabic words (HMWs) at a signal-to-noise ratio of 5 dB.

Results
Significant difference was observed between the mean scores of the WINP test in age group A (54%) compared to age group B (66%), (Pv = 0.04). Also, the differences between the mean scores of age group A compared to age groups C (70%) and D (69%) were significant (Pv = 0.01, Pv = 0.03).

Conclusions
This article presented the norm values of WINP test scores for Persian speaking Iranian children aged 5 to 13 years. The norm values of WINP test scores in the age group of 5–7 years were lower than in the age groups of 7–13 years. The biggest change in the performance of speech perception in noise was observed in the age range of 7–9 years.
Developing a coordinated response to chemsex across health, justice and social care settings: expert consensus statement
Authors: Hillier Bradley , Carthy Eliott , Kalk Nicola , Moncrieff Monty , Pakianathan Mark , Tracy Derek , Bowden-Jones Owen , Hickson Ford , Forrester Andrew 
Published: June 2024

SummaryChemsex occurs primarily among gay, bisexual and other men who have sex with men (GBMSM), and there is evidence of a subgroup of users who carry out chemsex-related criminal offences and experience harm. Challenges with chemsex can present to various settings; there are concerns that harm is increasing, including at interfaces between health, social care and criminal justice systems. The UK response to date has lacked a coordinated approach. An expert reference group was convened to share chemsex knowledge, articulate priorities for research and pathway development, and foster collaborative working between agencies. It made three key recommendations: develop and increase training and awareness across all services; implement a coordinated research programme with the development of a common data-set and assessment tool to fully characterise population-level needs; develop a professional network to share information, provide professional support and act as a knowledge hub. There was support for a unified multi-agency strategy incorporating the priorities identified as overarching principles.
Explainable Machine Learning Model to Predict Overall Survival in Patients Treated With Palliative Radiotherapy for Bone Metastases
Authors: Cilla Savino , Rossi Romina , Habberstad Ragnhild , Klepstad Pal , Dall’Agata Monia , Kaasa Stein , Valenti Vanessa , Donati Costanza M. , Maltoni Marco , Morganti Alessio G. 
Published: June 2024

PURPOSE The estimation of prognosis and life expectancy is critical in the care of patients with advanced cancer. To aid clinical decision making, we build a prognostic strategy combining a machine learning (ML) model with explainable artificial intelligence to predict 1-year survival after palliative radiotherapy (RT) for bone metastasis. MATERIALS AND METHODS Data collected in the multicentric PRAIS trial were extracted for 574 eligible adults diagnosed with metastatic cancer. The primary end point was the overall survival (OS) at 1 year (1-year OS) after the start of RT. Candidate covariate predictors consisted of 13 clinical and tumor-related pre-RT patient characteristics, seven dosimetric and treatment-related variables, and 45 pre-RT laboratory variables. ML models were developed and internally validated using the Python package. The effectiveness of each model was evaluated in terms of discrimination. A Shapley Additive Explanations (SHAP) explainability analysis to infer the global and local feature importance and to understand the reasons for correct and misclassified predictions was performed. RESULTS The best-performing model for the classification of 1-year OS was the extreme gradient boosting algorithm, with AUC and F1-score values equal to 0.805 and 0.802, respectively. The SHAP technique revealed that higher chance of 1-year survival is associated with low values of interleukin-8, higher values of hemoglobin and lymphocyte count, and the nonuse of steroids. CONCLUSION An explainable ML approach can provide a reliable prediction of 1-year survival after RT in patients with advanced cancer. The implementation of SHAP analysis provides an intelligible explanation of individualized risk prediction, enabling oncologists to identify the best strategy for patient stratification and treatment selection.
Chiari I Malformation With Concomitant Nonfunctioning Pituitary and Adrenal Tumors
Authors: Zielinski Rachel , Khan Aysha , Mirza Faryal Sardar 
Published: June 2024

Abstract
Chiari 1 malformation (CM1) is a rare finding that has been described with growth hormone (GH)-secreting pituitary adenomas and with an endothelial PAS domain protein 1 gain-of-function mutation syndrome. We describe the first reported case of a patient diagnosed with CM1 and nonfunctioning pituitary and adrenal incidentalomas. Our case describes a 45-year-old female who was found to have cerebellar tonsillar ectopia consistent with CM1, a pituitary tumor, and bilateral adrenal incidentalomas. She was diagnosed after presenting with 2 weeks of upper extremity weakness and paresthesia. A comprehensive endocrine workup including insulin like growth factor (IGF-1) was normal. She underwent posterior fossa decompression without complication. Pituitary adenectomy was not pursued as there was no evidence of compression of the chiasm or the surrounding structures. In previous case reports it has been proposed that GH-secreting adenomas contribute to CM1 by causing hypertrophy of soft tissue structures in the skull base, overcrowding the posterior fossa. Given that our patient had normal IGF-1 levels, there could be a different underlying mechanism that contributed to the concomitant occurrence of CM1 with the pituitary and adrenal tumors.
From Matter to Material Culture
Authors: Miller Maureen C. 
Published: June 2024

Abstract
As a contribution to the Common Knowledge symposium “Caroline Walker Bynum across the Disciplines,” this essay traces the origins and development of Bynum's interest in the material artifacts of late medieval Christian spirituality. The author narrates these evolutions through analyses of a single object, the Louvain beguine cradle from the collections of the Metropolitan Museum of Art. The essay begins by treating Bynum's research from the 1980s to the early 1990s as moving toward a “visual theology” and then charts her movement from an interest in matter to an interest in materiality over the second half of the 1990s and first decade of the twenty-first century. Finally, the author uses the Louvain cradle's appearance in Bynum's most recent book, Dissimilar Similitudes, to articulate and reflect upon her contribution to material culture studies.
Bynum across the Generations
Authors: Herzig Tamar , Elmakais Omer 
Published: June 2024

Abstract
In this afterword to the Common Knowledge symposium “Caroline Walker Bynum across the Disciplines,” Bynum's early work is seen to have revolutionized the fields of medieval studies and religious studies by disclosing the need to account for the embodied and gendered aspects of Christian spirituality. It reflects on the enduring influence of her book Holy Feast and Holy Fast on the study of premodern mysticism, sanctity, and witchcraft, then discusses the impact of Bynum's later works on the reception of Holy Feast and Holy Fast in the third decade of the twenty-first century.
Revocability, Exception, Disqualification
Authors: Deutscher Penelope 
Published: June 2024

Abstract
The Dobbs decision revoking the constitutional legality of abortion in the United States was widely characterized as a use of raw power. That gives rise to the questions: What kind of power is in question? How does the post-Dobbs moment encapsulate a number of hinges between formations of power characterized in post-Foucauldian theory? How is fluency in the combinations of power at work in the Dobbs decision and aftermath enhanced by a vocabulary of such hinges, including “revocability,” “exception,” and “disqualification”? The article opens up the relationship between rights-bearing and the differential distribution of expectations (for some) that rights be accompanied by particular kinds of good conduct from which others are exempt. Asking how and why abortion has served as a device for setting aside a broad range of rights, the article gives special attention to “exemption” and “self-exemption” in the reproductive governance of abortion.