bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a rapid and high‐performance chemiluminescence immunoassay based on magnetic particles for protein S100B in human serum
Authors: Zhang Huisheng , Qi Suwen , Rao Jie , Li Qiaoliang , Yin Li , Lu Yuejun 
Published: January 2013

ABSTRACTProtein S100B is a clinically useful non‐invasive biomarker for brain cell damage. A rapid chemiluminescence immunoassay (CLIA) for S100B in human serum has been developed. Fluorescein isothiocyanate (FITC) and N‐(aminobutyl)‐N‐(ethylisoluminol) (ABEI) are used to label two different monoclonal antibodies of anti‐S100B. Protein S100B in serum combines with labeled antibodies and can form a sandwiched immunoreaction. A simplified separation procedure based on the use of magnetic particles (MPs) that were coated with anti‐FITC antibody is performed to remove the unwanted materials. After adding the substrate solution, the relative light unit (RLU) of ABEI is measured and is found to be directly proportional to the concentration of S100B in serum. The relevant variables involved in the CLIA signals are optimized and the parameters of the proposed method are evaluated. The results demonstrate that the method is linear to 25 ng/mL S100B with a detection limit of 0.02 ng/mL. The coefficient of variation (CV) is < 5% and < 6% for intra‐ and interassay precision, respectively. The average recoveries are between 97 and 107%. The linearity–dilution effect produces a linear correlation coefficient of 0.9988. Compared with the commercial kit, the proposed method shows a correlation of 0.9897. The proposed method displays acceptable performance for quantification of S100B and is appropriate for use in clinical diagnosis. Copyright © 2013 John Wiley & Sons, Ltd.
Superoxide anion radical scavenging property of catecholamines
Authors: Kładna Aleksandra , Berczyński Paweł , Kruk Irena , Michalska Teresa , Aboul‐Enein Hassan Y. 
Published: January 2013

ABSTRACTThe direct effect of the four catecholamines (adrenaline, noradrenaline, dopamine and isoproterenol) on superoxide anion radicals () was investigated. The reaction between 18‐crown‐6‐ether and potassium superoxide in dimethylsulfoxide was used as a source of . The reactivity of catecholamines with was examined using chemiluminescence, reduction of nitroblue tetrazolium and electron paramagnetic resonance spin‐trapping techniques. 5,5‐Dimethyl‐1‐pyrroline‐N‐oxide was included as the spin trap. The results showed that the four catecholamines were effective and efficient in inhibiting chemiluminescence accompanying the potassium superoxide/18‐crown‐6‐ether system in a dose‐dependent manner over the range 0.05–2 mm in the following order: adrenaline > noradrenaline > dopamine > isoproterenol, with, IC50 = 0.15 ± 0.02 mm 0.21 ± 0.03 mm, 0.27 ± 0.03 mm and 0.50 ± 0.04 mm, respectively. The catecholamines examined also exhibited a strong scavenging effect towards when evaluated this property by the inhibition of nitroblue tetrazolium reduction (56–73% at 1 m concentration). A very similar capacity of scavenging was monitored in the 5,5‐dimethyl‐1‐pyrroline‐N‐oxide spin‐trapping assay. The results suggest that catecholamines tested may involve a direct effect on scavenging radicals. Copyright © 2013 John Wiley & Sons, Ltd.
Monitoring anaerobic sequential batch reactors via fractal analysis of pH time series
Authors: Méndez‐Acosta H.O. , Hernandez‐Martinez E. , Jáuregui‐Jáuregui J.A. , Alvarez‐Ramirez J. , Puebla H. 
Published: January 2013

AbstractEfficient monitoring and control schemes are mandatory in the current operation of biological wastewater treatment plants because they must accomplish more demanding environmental policies. This fact is of particular interest in anaerobic digestion processes where the availability of accurate, inexpensive, and suitable sensors for the on‐line monitoring of key process variables remains an open problem nowadays. In particular, this problem is more challenging when dealing with batch processes where the monitoring strategy has to be performed in finite time, which limits the application of current advanced monitoring schemes as those based in the proposal of nonlinear observers (i.e., software sensors). In this article, a fractal time series analysis of pH fluctuations in an anaerobic sequential batch reactor (AnSBR) used for the treatment of tequila vinasses is presented. Results indicated that conventional on‐line pH measurements can be correlated with off‐line determined key process variables, such as COD, VFA and biogas production via some fractality indexes. Biotechnol. Bioeng. 2013; 110: 2131–2139. © 2013 Wiley Periodicals, Inc.
Comparison of three lymph node staging schemes for predicting outcome in patients with gastric cancer
Authors: Wang X , Appleby D-H , Zhang X , Gan L , Wang J-J , Wan F 
Published: January 2013

Abstract

Background
Several node staging schemes have been proposed for gastric cancer. The optimal system remains controversial.

Methods
Patients with gastric cancer were identified from the Surveillance, Epidemiology, and End Results (SEER) database, and a Chinese patient cohort was used for independent validation. The prognostic performance of three node staging schemes was compared, involving a number-based scheme (pN), ratio-based scheme (rN) and log odds of positive lymph nodes scheme (LODDS).

Results
There were 12 443 patients in the SEER database and 866 in the Chinese cohort. LODDS provided better discriminatory capacity and higher predictive accuracy than either pN or rN, for patients with gastric cancer in both the SEER database and the Chinese cohort. The multivariable model using the LODDS classification was significantly more predictive than the pN classification. LODDS suffered much less from stage migration and was able efficiently to discriminate the heterogeneity for patients with no nodes involved or all nodes involved, whereas the pN and rN schemes could not.

Conclusion
LODDS showed a clear prognostic superiority over both pN and rN schemes. It could serve as an important reference for the tumour node metastasis (TNM) node classification.
Rapid identification of primary constituents in parotoid gland secretions of the Australian cane toad using HPLC/MS‐Q‐TOF
Authors: Jing Jing , Ren Wei C. , Li Chun , Bose Utpal , Parekh Harendra S. , Wei Ming Q. 
Published: January 2013

ABSTRACTToad parotoid gland secretion or toad venom has in recent years been increasingly shown to possess potentially beneficial pharmacological effects; this speculation has drawn much interest centred on elucidating the chemical basis of its multimodal effects. For this purpose, we explored the use of a rapid and accurate analysis method for systemic investigation of the parotoid gland chemistry, when extracted from Australian cane toads. Full‐scan data of cane toad venom extract was acquired using high‐performance liquid chromatography coupled with a hybrid quadrupole–time of flight mass spectrometry system (HPLC/MS‐Q‐TOF), with multiple ionization sources (ESI and APCI) in positive and negative mixed modes. By measuring the exact mass differences between the theoretical and measured mass of each assumed compound, we confirmed the presence of 12 key constituents. The present results demonstrate that the use of HPLC/MS‐Q‐TOF with multiple ionization sources delivers exemplary selectivity and sensitivity, allowing for the rapid and accurate identification of constituents within cane toad venom. This paves the way for this technique to be used in future routine screening of components within the genus Bufo and for key analytes too, then reliably assessed for any purported beneficial (clinic) properties. Copyright © 2013 John Wiley & Sons, Ltd.
Gas‐Phase Reactions of Cationic Vanadium‐Phosphorus Oxide Clusters with C2Hx (x=4, 6): A DFT‐Based Analysis of Reactivity Patterns
Authors: Dietl Nicolas , Zhang Xinhao , van der Linde Christian , Beyer Martin K. , Schlangen Maria , Schwarz Helmut 
Published: January 2013

AbstractThe reactivities of the adamantane‐like heteronuclear vanadium‐phosphorus oxygen cluster ions [VxP4−xO10].+ (x=0, 2–4) towards hydrocarbons strongly depend on the V/P ratio of the clusters. Possible mechanisms for the gas‐phase reactions of these heteronuclear cations with ethene and ethane have been elucidated by means of DFT‐based calculations; homolytic CH bond activation constitutes the initial step, and for all systems the PO. unit of the clusters serves as the reactive site. More complex oxidation processes, such as oxygen‐atom transfer to, or oxidative dehydrogenation of the hydrocarbons require the presence of a vanadium atom to provide the electronic prerequisites which are necessary to bring about the 2e− reduction of the cationic clusters.
Nitrogen‐Doped Carbon Dots: A Facile and General Preparation Method, Photoluminescence Investigation, and Imaging Applications
Authors: Xu Yang , Wu Ming , Liu Yang , Feng Xi‐Zeng , Yin Xue‐Bo , He Xi‐Wen , Zhang Yu‐Kui 
Published: January 2013

AbstractCarbon dots (Cdots) are an important probe for imaging and sensing applications because of their fluorescence property, good biocompatibility, and low toxicity. However, complex procedures and strong acid treatment are often required and Cdots suffer from low photoluminescence (PL) emission. Herein, a facile and general strategy using carbonization of precursors and then extraction with solvents is proposed for the preparation of nitrogen‐doped Cdots (N‐Cdots) with 3‐(3,4‐dihydroxyphenyl)‐L‐alanine (L‐DOPA), L‐histidine, and L‐arginine as precursor models. After they are heated, the precursors become carbonized. Nitrogen‐doped Cdots are subsequently extracted into N,N′‐dimethylformamide (DMF) from the carbogenic solid. A core–shell structure of Cdots with a carbon core and the oxygen‐containing shell was observed. Nitrogen has different forms in N‐Cdots and oxidized N‐Cdots. The doped nitrogen and low oxidation level in N‐Cdots improve their emission significantly. The N‐Cdots show an emission with a nitrogen‐content‐dependent intensity and Cdot‐size‐dependent emission‐peak wavelength. Imaging of HeLa cells, a human cervical cancer cell line, and HepG2 cells, a human hepatocellular liver carcinoma line, was observed with high resolution using N‐Cdots as a probe and validates their use in imaging applications and their multicolor property in the living cell system.
Two Enantiomorphic MgII/Carboxylate Coordination Polymers Produced from Spontaneous Resolution with an Achiral Bipyridyl Dicarboxylate Ligand
Authors: Zhang Qingfu , Hao Hongguo , Zhang Haina , Wang Suna , Jin Juan , Sun Dezhi 
Published: January 2013

AbstractTwo enantiomorphic MgII/carboxylate coordination polymers [Mg(bpda)(H2O)4]n (1M and 1P) have been obtained by spontaneous resolution from achiral precursors, 2,2′‐bipyridyl‐3,3′‐dicarboxylic acid (H2bpda) and Mg(NO3)2·6H2O, upon crystallization without any chiral auxiliary. X‐ray structural analysis shows that chiral transmission from molecular axially chiral conformations to crystal chirality is established through concerted coordination and H‐bonding interactions. In addition, the CD data, and the thermal and photoluminescence properties in the solid state have also been discussed.
Planar Chiral Triazole‐Based Phosphanes Derived from [2.2]Paracyclophane and Their Activity in Suzuki Coupling Reactions
Authors: Glover Jean E. , Martin David J. , Plieger Paul G. , Rowlands Gareth J. 
Published: January 2013

AbstractIn this communication we report the short synthesis of a variety of planar chiral triazole‐based phosphanes. These molecules have been readily prepared from 4‐azido[2.2]paracyclophane in two steps. It was observed that the stability of the resulting phosphanes was dependent on the C‐4 substituent. The efficacy of the new phosphanes in the palladium‐mediated Suzuki–Miyaura coupling reaction was evaluated. The phosphanes proved to be more effective than previously prepared planar chiral [2.2]paracyclophane‐based phosphanes.
Exploring Properties of a Hyperthermophilic Membrane‐Bound Hydrogenase at Carbon Nanotube Modified Electrodes for a Powerful H2/O2 Biofuel Cell
Authors: De Poulpiquet Anne , Ciaccafava Alexandre , Szot Katarzyna , Pillain Baptiste , Infossi Pascale , Guiral Marianne , Opallo Marcin , Giudici‐Orticoni Marie‐Thérèse , Lojou Elisabeth 
Published: January 2013

AbstractHydrogenases are the key enzymes for hydrogen metabolism in many microorganisms. Due to the high efficiency and specificity they developed for H2 oxidation, they have been recently used as biocatalysts for an efficient H2/O2 biofuel cell. In this work we explore new properties of an O2‐, CO‐ and T°‐resistant hydrogenase. Enzyme immobilization on carbon nanotube modified electrodes is studied and optimized for long‐term stabilization of the direct hydrogen catalytic oxidation process. The role of co‐immobilized redox mediator is finally discussed in view of H2/O2 fuel cell performance enhancement.
Determination of Trichloroacetic Acid (TCAA) Using CdO Nanoparticles Modified Carbon Paste Electrode
Authors: Najafi Mostafa , Darabi Salman , Tadjarodi Azadeh , Imani Mina 
Published: January 2013

AbstractIn this paper, the electrochemical behavior of a carbon paste electrode modified with CdO nanoparticles as a potential electrocatalyst for the reduction of trichloroacetic acid (TCAA) was investigated using cyclic voltammetry and double‐potential step chronoamperometry. The modified electrode showed a great enhancement in cathodic peak current with respect to reduction of TCAA in acidic aqueous solution. Using this increment, a quantitative method was developed for the determination of TCAA in aqueous solution. The detection limit and linear dynamic range of TCAA are 2.3×10−6 M and 2.3×10−4–3×10−6 M, respectively.
Detection of Prunus Necrotic Ringspot Virus in Plant Extracts with Impedimetric Immunosensor based on Glassy Carbon Electrode
Authors: Jarocka Urszula , Radecka Hanna , Malinowski Tadeusz , Michalczuk Lech , Radecki Jerzy 
Published: January 2013

AbstractThis paper concerns the development of an immunosensor for detection of Prunus necrotic ringspot virus (PNRSV) in plant extracts. The immunosensor fabrication consists of successive modification steps of glassy carbon electrodes: (i) creation of COOH groups, (ii) covalent immobilization of protein A with EDC/NHS coupling reaction, (iii) immobilization of anti‐PNRSV IgG polyclonal antibody, (iiii) filling free spaces with BSA. Each step was controlled with cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The immunosensor was applied for the determination of the PNRSV virus in extracts from cucumber leaves using EIS technique. It was capable of discriminating between samples consisting of extracts from healthy plants and consisting of leaf extracts from infected plants diluted 10 000 times with extract from healthy plants.
Detection of Tellurium in Presence of Extra Arsenic on Gold Electrodes Modified By Benzenedithiol Self‐Assembled Monolayers
Authors: Bass Ethan , Ke Shan 
Published: January 2013

AbstractElectrochemical detection of tellurium is interfered with arsenic due to their similar redox potentials. Gold electrodes modified by benzenedithiols (BDT) self‐assembled monolayers (SAMs) were explored to determine Te(IV) in the presence of extra As(III) in this work. SAMs of benzenedithiols on the gold surface allows for selective extraction of Te (IV) over As(III), while blocking the redox of As(III). It provides potential applications to tellurium toxicity analysis, biological membrane study and identification of geochemistry samples.
Cross-tolerance in amphibians: Wood frog mortality when exposed to three insecticides with a common mode of action
Authors: Hua Jessica , Cothran Rickey , Stoler Aaron , Relyea Rick 
Published: January 2013

Abstract
Insecticide tolerance and cross-tolerance in nontarget organisms is often overlooked despite its potential to buffer natural systems from anthropogenic influence. We exposed wood frog tadpoles from 15 populations to three acetylcholine esterase-inhibiting insecticides and found widespread variation in insecticide tolerance and evidence for cross-tolerance to these insecticides. Our results demonstrate that amphibian populations with tolerance to one pesticide may be tolerant to many other pesticides. Environ. Toxicol. Chem. 2013;32:932–936. © 2013 SETAC
Associations between perfluoroalkyl compounds and immune and clinical chemistry parameters in highly exposed bottlenose dolphins (Tursiops truncatus)
Authors: Fair Patricia A. , Romano Tracy , Schaefer Adam M. , Reif John S. , Bossart Gregory D. , Houde Magali , Muir Derek , Adams Jeff , Rice Charles , Hulsey Thomas C. , Peden-Adams Margie 
Published: January 2013

Abstract
Perfluoroalkyl compounds (PFCs) are ubiquitous, persistent chemical contaminants found in the environment, wildlife, and humans. Despite the widespread occurrence of PFCs, little is known about the impact these contaminants have on the health of wildlife populations. The authors investigated the relationship between PFCs (including ∑perfluorocarboxylates, ∑perfluoroalkyl sulfonates, perfluorooctane sulfonate, perfluorooctanoic acid, and perfluorodecanoic acid) and the clinocopathologic and immune parameters in a highly exposed population (n = 79) of Atlantic bottlenose dolphins (mean ∑PFCs = 1970 ng/ml; range 574–8670 ng/ml) sampled from 2003 to 2005 near Charleston, South Carolina, USA. Age-adjusted linear regression models showed statistically significant positive associations between exposure to one or more of the PFC totals and/or individual analytes and the following immunological parameters: absolute numbers of CD2+ T cells, CD4+ helper T cells, CD19+ immature B cells, CD21+ mature B cells, CD2/CD21 ratio, MHCII+ cells, B cell proliferation, serum IgG1, granulocytic, and monocytic phagocytosis. Several PFC analyte groups were also positively associated with serum alanine aminotransferase, gamma-glutamyltransferase, creatinine, phosphorus, amylase, and anion gap and negatively associated with cholesterol levels, creatinine phosphokinase, eosinophils, and monocytes. Based on these relationships, the authors suggest that the PFC concentrations found in Charleston dolphins may have effects on immune, hematopoietic, kidney, and liver function. The results contribute to the emerging data on PFC health effects in this first study to describe associations between PFCs and health parameters in dolphins. Environ. Toxicol. Chem. 2013;32:736–746. © 2013 SETAC
Predictive QSPR modelling for the olfactory threshold of a series of pyrazine derivatives
Authors: Pal Pallabi , Mitra Indrani , Roy Kunal 
Published: January 2013

ABSTRACTNowadays, much interest is shown in the prediction of different organoleptic properties of molecules for their prominent use in a variety of formulations and food products. Against this background, cheminformatics modelling was done in the present work for the development of predictive models for the olfactory threshold (log T) of a series of 74 pyrazine derivatives employing in silico techniques such as genetic function algorithm (GFA) and genetic partial least squares algorithm (G/PLS). Different categories of descriptors were calculated for the work. After validating the models both internally and externally, a parabolic GFA‐spline model was selected as the best model which showed significant predictive quality [ = 0.822,
= 0.641,
= 0.019]. Interpretation of the descriptors concluded that increased hydrophobic surface area, along with an increase in the positive charge weighted solvent accessible surface area (as interpreted from the Jurs_DPSA_2 descriptor) in the molecules, provides a low value of log T. Again, the presence of an isopropyl group and electron richness of the molecules significantly influences the property profile (log T) of the molecules. Finally, 27 pyrazine derivatives were designed based on the present analysis and good in silico prediction values for odour threshold, i.e. low log T values were obtained from the developed model. Thus, use of this optimized quantitative structure–property relationship (QSPR) model may be used to screen molecule databases as well as modify the structures for selection of potent entities. Copyright © 2013 John Wiley & Sons, Ltd.
Identification of novel serum biomarkers of hepatocellular carcinoma using glycomic analysis
Authors: Kamiyama Toshiya , Yokoo Hideki , Furukawa Jun-Ichi , Kurogochi Masaki , Togashi Tomoaki , Miura Nobuaki , Nakanishi Kazuaki , Kamachi Hirofumi , Kakisaka Tatsuhiko , Tsuruga Yosuke , Fujiyoshi Masato , Taketomi Akinobu , Nishimura Shin-Ichiro , Todo Satoru 
Published: January 2013

Abstract

The altered

N

-glycosylation of glycoproteins has been suggested to play an important role in the behavior of malignant cells. Using glycomics technology, we attempted to determine the specific and detailed

N

-glycan profile for hepatocellular carcinoma (HCC) and investigate the prognostic capabilities. From 1999 to 2011, 369 patients underwent primary curative hepatectomy in our facility and were followed up for a median of 60.7 months. As normal controls, 26 living Japanese related liver transplantation donors were selected not infected by hepatitis B and C virus. Their mean age was 40.0 and 15 (57.7%) were male. We used a glycoblotting method to purify

N

-glycans from preoperative blood samples from this cohort (10 μL serum) which were then identified and quantified using mass spectrometry (MS). Correlations between the

N

-glycan levels and the clinicopathologic characteristics and outcomes for these patients were evaluated. Our analysis of the relative areas of all the sugar peaks identified by MS, totaling 67

N

-glycans, revealed that a proportion had higher relative areas in the HCC cases compared with the normal controls. Fourteen of these molecules had an area under the curve of greater than 0.80. Analysis of the correlation between these 14

N

-glycans and surgical outcomes by univariate and multivariate analysis identified G2890 (

m/z

value, 2890.052) as a significant recurrence factor and G3560 (

m/z

value, 3560.295) as a significant prognostic factor. G2890 and G3560 were found to be strongly correlated with tumor number, size, and vascular invasion.

Conclusion

: Quantitative glycoblotting based on whole serum

N

-glycan profiling is an effective approach to screening for new biomarkers. The G2890 and G3560

N

-glycans determined by tumor glycomics appear to be promising biomarkers for malignant behavior in HCCs.