bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro-C reveals MORC/ApiAP2-mediated links between distant, functionally related genes in the human malaria parasite
Authors: Singh Parul , Serizay Jacques , Couble Justine , Cabahug Maureen D. , Rosa Catarina , Chen Patty , Scherf Artur , Koszul Romain , Baumgarten Sebastian , Bryant Jessica M. 
Published: August 2024

SummaryGenome organization plays a significant role in silencing heterochromatinized genes in the most virulent human malaria parasite,Plasmodium falciparum. However, it remains unclear how heterochromatinized genes spatially cluster or if active genes are also organized in a specific manner. We used Micro-C to achieve a near-nucleosome resolution DNA-DNA contact map, which revealed new inter- and intrachromosomal heterochromatic and euchromatic structures in the blood stage parasite. We observed subtelomeric fold structures that facilitate interactions amongst heterochromatinized genes involved in antigenic variation. In addition, we identified long-range intra- and interchromosomal interactions amongst active, stage-specific genes. Both structures are mediated by AP2-P, an ApiAP2 transcription factor, and a putative MORC chromatin remodeler, and functional specificity is achieved via combinatorial binding with other sequence-specific DNA-binding factors. This study provides unprecedented insight into the organizational machinery used by this medically important eukaryotic parasite to spatially coordinate genes underlying antigenic variation and to co-activate stage-specific genes.
Chromosome-level genome assembly and methylome profile enables insights for the conservation of endangered loggerhead sea turtles
Authors: Yen Eugenie C. , Gilbert James D. , Balard Alice , Taxonera Albert , Fairweather Kirsten , Ford Heather L. , Thorburn Doko-Miles J. , Rossiter Stephen J. , Martín-Durán José M. , Eizaguirre Christophe 
Published: August 2024

ABSTRACTBackgroundCharacterising genetic and epigenetic diversity is crucial for assessing the adaptive potential of populations and species. Slow-reproducing and already threatened species, including endangered sea turtles, are particularly at risk. Those species with temperature-dependent sex determination (TSD) have heightened climate vulnerability, with sea turtle populations facing feminisation and extinction under future climate change. High- quality genomic and epigenomic resources will therefore support conservation efforts for these flagship species with such plastic traits.FindingsWe generated a chromosome-level genome assembly for the loggerhead sea turtle (Caretta caretta) from the globally important Cabo Verde rookery. Using Oxford Nanopore Technology (ONT) and Illumina reads followed by homology-guided scaffolding, we achieved a contiguous (N50: 129.7 Mbp) and complete (BUSCO: 97.1%) assembly, with 98.9% of the genome scaffolded into 28 chromosomes and 29,883 annotated genes. We then extracted the ONT-derived methylome and validated it via whole genome bisulfite sequencing of ten loggerheads from the same population. Applying our novel resources, we reconstructed population size fluctuations and matched them with major climatic events and niche availability. We identified microchromosomes as key regions for monitoring genetic diversity and epigenetic flexibility. Isolating 191 TSD-linked genes, we further built the largest network of functional associations and methylation patterns for sea turtles to date.ConclusionsWe present a high-quality loggerhead sea turtle genome and methylome from the globally significant East Atlantic population. By leveraging ONT sequencing to create genomic and epigenomic resources simultaneously, we showcase this dual strategy for driving conservation insights into endangered sea turtles.
Dopamine and temporal discounting: revisiting pharmacology and individual differences
Authors: Smith Elke , Theis Hendrik , van Eimeren Thilo , Knauth Kilian , Tuzsus Deniz , Zhang Lei , Mathar David , Peters Jan 
Published: August 2024

AbstractDisorders characterised by changes in dopamine (DA) neurotransmission are often linked to changes in the temporal discounting of future rewards. Likewise, pharmacological manipulations of DA neuro-transmission in healthy individuals modulate temporal discounting, but there is considerable variability in the directionality of reported pharmacological effects, as enhancements and reductions of DA signalling have been linked to both increases and reductions of temporal discounting. This may be due to meaningful individual differences in drug effects and/or false positive findings in small samples. To resolve these inconsistencies, we 1) revisited pharmacological effects of the DA precursor L-DOPA on temporal discounting in a large sample of N = 76 healthy participants (n = 44 male) and 2) examined several putative proxy measures for DA to revisit the role of individual differences in a randomised, double-blind placebo-controlled pre-registered study (https://osf.io/a4k9j/). Replicating previous findings, higher rewards were discounted less (magnitude effect). Computational modelling using hierarchical Bayesian parameter estimation confirmed that the data in both drug conditions were best accounted for by a non-linear temporal discounting drift diffusion model. In line with recent animal and human work, L-DOPA reliably reduced the discount rate with a small effect size, challenging earlier findings in substantially smaller samples. We found no credible evidence for linear or quadratic effects of putative DA proxy measures on model parameters, calling into question the role of these measures in accounting for individual differences in DA drug effects.
Cellular processing of beneficialde novoemerging proteins
Authors: Houghton Carly J. , Coelho Nelson Castilho , Chiang Annette , Hedayati Stefanie , Parikh Saurin B. , Ozbaki-Yagan Nejla , Wacholder Aaron , Iannotta John , Berger Alexis , Carvunis Anne-Ruxandra , O’Donnell Allyson F. 
Published: August 2024

AbstractNovel proteins can originatede novofrom non-coding DNA and contribute to species-specific adaptations. It is challenging to conceive howde novoemerging proteins may integrate pre-existing cellular systems to bring about beneficial traits, given that their sequences are previously unseen by the cell. To address this apparent paradox, we investigated 26de novoemerging proteins previously associated with growth benefits in yeast. Microscopy revealed that these beneficial emerging proteins preferentially localize to the endoplasmic reticulum (ER). Sequence and structure analyses uncovered a common protein organization among all ER-localizing beneficial emerging proteins, characterized by a short hydrophobic C-terminus immediately preceded by a transmembrane domain. Using genetic and biochemical approaches, we showed that ER localization of beneficial emerging proteins requires the GET and SND pathways, both of which are evolutionarily conserved and known to recognize transmembrane domains to promote post-translational ER insertion. The abundance of ER-localizing beneficial emerging proteins was regulated by conserved proteasome- and vacuole-dependent processes, through mechanisms that appear to be facilitated by the emerging proteins’ C-termini. Consequently, we propose that evolutionarily conserved pathways can convergently govern the cellular processing ofde novoemerging proteins with unique sequences, likely owing to common underlying protein organization patterns.
Measurement model of readiness for online testing of undergraduate students in Thailand’s distance education programs
Authors: Laosum Thanyasinee 
Published: August 2024

PurposeThis study aims to develop a model for readiness measurement and to study readiness levels for online testing of undergraduate students in Thailand’s distance education programs.Design/methodology/approachIn total, 870 undergraduate students enrolled in the 2022 academic year of a Thai university were sampled for the study. The samples were divided into two groups: Group 1 comprised 432 students who underwent exploratory factor analysis (EFA) and Group 2 comprised 438 students who underwent second-order confirmatory factor analysis (CFA). Both were multi-stage random samples. Descriptive statistics, item-total correlations (ITCs), coefficient correlations, EFA and second-order CFA were used.FindingsThe readiness for the online testing model comprised 5 factors and 33 indicators. These included self-efficacy (SE) in utilizing technology (nine indicators), self-directed learning (SL) for readiness testing (six indicators), adequacy of technology (AT) for testing (five indicators), acceptance of online testing (AC) (seven indicators) and readiness training for testing (six indicators). The model was congruent with empirical data, and the survey results indicated that students were highly prepared at the “high” level.Practical implicationsThis study disclosed several factors and indicators involved in the readiness for online testing. The university may use these findings in preparing its students for online testing for better achievement.Originality/valueThese findings may serve as a framework for the analysis of the readiness issues for online testing of undergraduate students and also offer guidance to the universities preparing to offer online testing.
Cloud-based Building Information Modelling (CBIM): software engineers' insights into improving asset information quality
Authors: Chang Janet , Xie Xiang , Parlikad Ajith Kumar 
Published: August 2024

PurposeThis research investigates the capabilities of Cloud-based Building Information Modelling (CBIM) in managing quality asset information, drawing upon software engineers' perspectives. Compelling statistics highlight the relationship between building information and environmental sustainability. However, despite the growing utilisation of CBIM in the Architecture, Engineering and Construction (AEC) industry, a significant knowledge gap remains concerning its effectiveness in maintaining quality asset information.Design/methodology/approachThis study employed an exploratory qualitative approach, utilising semi-structured interviews with thirteen software engineers actively developing technological solutions for the AEC industry. Following thematic analysis, the findings are categorised into four dimensions: strengths, weaknesses, opportunities and technological limitations. Subsequently, these findings are analysed in relation to previously identified information quality problems.FindingsThis research reveals that while CBIM improves project coordination and information accessibility, its effectiveness is challenged by the need for manual updates, vulnerability to human errors and dependency on network services. Technological limitations, notably the absence of automated updates for as-built drawings and the risk of data loss during file conversions in the design phase, coupled with its reduced capability to validate context-specific information from the user's viewpoint, emphasise the urgent need for managerial strategies to maximise CBIM's capabilities in addressing information quality problems.Originality/valueThis study augments the understanding of CBIM, highlighting the managerial implications of a robust information management process to safeguard information integrity. This approach fosters sustainable practices anchored in reliable information essential for achieving desired outcomes. The findings also have broader managerial implications, especially for sectors that employ CBIM as an instrumental tool.
Are construction enterprises with higher niches more willing to implement compliance behavior?
Authors: Wang Zhixiu , Ye Kunhui 
Published: August 2024

PurposeConstruction enterprises increase their competitive advantage by joining the project ecosystem, but the dual nature of the enterprise’s niche has attracted attention, and existing research has gaps in understanding niche and ecosystem governance issues. This study aims to promote ecosystem compliance governance by exploring the impact of the niche of the key role of construction enterprises on compliance behavior.Design/methodology/approachFirst, the study proposes a model on the impact of the enterprise's niche on its compliance behavior and the mediating role of the enterprise's perception of sanctions and the moderating role of a shared vision for compliance of ecological partners within these relationships. Second, we used 205 samples, who were Chinese contractors with international construction project experience through a questionnaire survey. Third, the study conducted a hierarchical regression to test the hypothesis.FindingsThe results show that construction enterprises with a wider niche or with a higher degree of niche overlap are more inclined to performance compliance. Enterprises' perception of sanctions plays a part in mediating the enterprise's niche and compliance behavior. The relationship between enterprises' perception of sanctions and compliance behavior can be moderated by the shared vision for compliance of ecological partners. Given a low compliance shared vision of ecological partners, the impact of enterprise perception of sanctions on compliance behavior is positively stronger.Practical implicationsThe findings provide valuable evidence upon which ecosystem governance needs to focus and leverage the role of key members, using the advantageous resources of key members as a fulcrum to leverage a larger governance scope. Construction enterprises should keep improving their niche and the shared vision for partners' compliance to promote the evolution and upgrading of cooperation to an ecosystem model that creates greater value.Originality/valueThis study provides new insights for future compliance governance in the project ecosystem by introducing the concept of niche and answering whether construction enterprises with a higher niche in the project ecosystem are more willing to implement compliance behavior.
Brewing success: Woolah’s bagless tea dip for sustainable sipping
Authors: Nigam Namita , Mohanty Devi Archana , Agarwal Puja Shree 
Published: August 2024

Learning outcomes
After completion of the case study, students will be able to identify the strategic key components of the Woolah tea brand through the business model canvas framework, to evaluate the major challenges faced by different stakeholders, to analyse the potential effects of bagless tea dip innovation and understand the principles of design thinking and its application in developing innovative solutions, to assess the strategic framework of Woolah tea brand to scale up its business and operations and to align the Woolah tea brand’s sustainability practices with a triple bottom line approach and contribution to UN Sustainable Development Goals.

Case overview/synopsis
Upamanyu Borkakoty and Anshuman Bharali, the founder duo of Woolah tea, began their entrepreneurial journey on a noble note. They recognised that plastic tea bags, which customers worldwide consume, create harmful health effects in the form of microplastic. They aimed to provide an authentic and sustainable tea experience while making it microplastic-free. When the world is heading towards securing a sustainable future, they envisioned adding a feather to it by proposing their Truedips. The USP of their product is Truedips – a tea ball they prepare by compressing one bud and two premier leaves. The founders were convinced that their innovative idea of tea consumption would provide customers with an authentic and exhilarating experience. However, there were dilemmas and roadblocks. They faced roadblocks related to the farmer’s traditional approach to growing tea, untrained tea growers, lack of financial assistance and customer readiness for a bagless tea experience. The dilemmas they faced related to their customer acceptance of their idea and the price affordability of the product. The big question hovering around was the customer’s feedback and acceptance of the product.

Complexity academic level
This case study suits graduate and postgraduate business administration students and other management programmes. The case study can also be used for business, marketing, design thinking, innovation and and social entrepreneurship courses.

Supplementary materials
Teaching notes are available for educators only.

Subject code
CSS 3: Entrepreneurship.
Bridging power and fairness: unravelling the impact of informal networks on organisational justice in the ARAB region
Authors: Al-Twal Arwa , Alsarhan Fadi , Nabulsi Leen , Horani Christina 
Published: August 2024

PurposeWasta, the prevailing form of informal networks in the Arab region, exerts a significant controversial influence on organisations in this region. Despite the pervasive negative perceptions and alleged detrimental effects of Wasta, it remains a frequently employed practice. This research aims to comprehensively explore the influence of Wasta on organisational justice across organisations in the Arab world, with Jordanian organisations taken as an illustrative case.Design/methodology/approachEmploying a qualitative approach, semi-structured interviews were conducted with 20 employees from various industries in Jordan.FindingsThe study reveals that Wasta significantly affects distributive, procedural and interactional justice within organisations, eliciting feelings of unfairness and hopelessness among employees.Practical implicationsThis study recommends that HR departments focus on implementing ethical policies and standards to enhance transparency in organisational decision-making processes. By understanding and addressing the implications of Wasta, organisations can foster a more just and equitable workplace environment.Originality/valueThis study advances our understanding of informal network theories in the Arab world by highlighting the interconnectedness between Wasta and organisational justice, and its profound impact on the work environment and employees. This study also explains how Wasta directly influences decision-making processes, at high managerial levels.
Service satisfaction among a language minority: a randomized survey experiment on the satisfaction of Swedish-speaking Finns with early childhood education
Authors: Vento Isak , Eklund Jesper , Schauman Jonas 
Published: August 2024

PurposeThis study explores the effect of language on service satisfaction among Finland-Swedes, a national minority language group in Finland, in the context of early childhood education. Models of public service satisfaction hold standard process and outcome related factors, such as availability and quality, as drivers of the satisfaction. However, although research has shown significant variation in satisfaction between different groups of citizens (race, ethnicity, age etc.), research has largely overlooked group specific factors as explanations for the satisfaction.Design/methodology/approachA randomized survey experiment with a 2 × 2 × 2 factorial design analyzed the impacts of language, service accessibility, and quality on service satisfaction. The data was analyzed with ANOVA.FindingsThe results revealed that language significantly impacts Swedish speakers’ satisfaction, suggesting that for minority groups, language may override typical satisfaction determinants like quality and accessibility. Interestingly, special linguistic needs are relatively more pertinent in low-quality services than in higher-quality ones.Originality/valueThe study shows how group related factors of public service, in our case language, in an important factor explaining satisfaction with the service. The findings have implications for the literature on citizens’ satisfaction with public services with demographic and identity facets, especially in a typical Nordic welfare state.
A low-emission pathway for tourism passenger transport in small and medium-sized urban destinations – case of Yilan, Taiwan
Authors: Lin Yung-Hsin , Nitivattananon Vilas 
Published: August 2024

Purpose
The nexus of transport and tourism is critical to the 2021 Glasgow Declaration which sets out the net zero by 2050 goal for global tourism in the context of the Paris Agreement. Numerous small and medium-sized urban destinations (SMUDs) populated under one million are constrained by a limited capacity to manage visitor flows and increasing greenhouse gas (GHG) emissions. This paper aims to develop an analytical approach for urban practitioners, based on a case study in Taiwan, to identify the low-emission pathway and strategies for tourism passenger transport.

Design/methodology/approach
A GHG emissions assessment and scenario analysis were enabled by historical activity data from official sources and projected scenario data from the International Energy Agency. The scenarios were established based on the avoid-shift-improve framework for low-carbon transport.

Findings
To drive tourism passenger transport to a low-emission pathway compatible with the Paris Agreement goal, three low-carbon transport strategies, i.e. “Avoid,” “Shift” and “Improve,” shall be applied all together, with a focus on “improving” the efficiency of heavy-duty vehicles and rail transport. Meanwhile, alternative tourism and integrated transport policy packages could enhance demand-side management of visitors’ mobility, enabling the “avoid” and “shift” strategies.

Originality/value
Unlike most studies that have focused on large cities or small tourist areas, this paper addressed our knowledge gap regarding the low-emission pathway for tourism transport in numerous SMUDs compatible with a 1.5°C world. The proposed analytical approach can help policymakers assess effective strategies toward the targeted pathway.
Managing knowledge to enable learning individuals with intellectual and developmental disabilities: the missing piece on knowledge management
Authors: Almuaqel Ibraheem Abdulaziz 
Published: August 2024

Purpose
This study aims to present a qualitative analysis of how higher education institutions (HEIs) faculties manage knowledge to facilitate the learning and engagement of individuals with intellectual and developmental disabilities.

Design/methodology/approach
This study uses a qualitative research design to collect responses from 39 HEI faculties to understand how they manage knowledge to facilitate learning in individuals with intellectual and developmental disabilities. Data collection tools comprised a set of predetermined questions, soliciting written responses.

Findings
Coding of the collected data confirmed that there was a knowledge management process in HEIs for enabling higher education of individuals with intellectual and developmental disabilities. A total of six themes emerged, clarifying the knowledge management process. The four components of this process were creation, storage, sharing and use. In addition to the four process-related themes, two other themes that emerged were barriers to knowledge creation and supportive culture. This covers the relevant aspects of the set-up around the knowledge management process in HEIs trying to improve the higher education of individuals with intellectual and developmental disabilities.

Originality/value
There is a need to improve the education of individuals with intellectual and developmental disabilities, which requires effective knowledge management. This paper reveals details of the steps in the knowledge management process relevant to this aim. This is a unique contribution, providing a basis for future research and the introduction of required knowledge management practices by HEIs dedicated to providing high-quality education to students with intellectual and developmental disabilities.