bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protective effects of schizandrin and schizandrin B towards cisplatin nephrotoxicity in vitro
Authors: Bunel Valérian , Antoine Marie‐Hélène , Nortier Joëlle , Duez Pierre , Stévigny Caroline 
Published: October 2013

ABSTRACTRenal proximal tubular epithelial cells are the main targets of toxic drugs such as cisplatin (CisPt), an alkylating agent indicated for the treatment of solid organ tumors. Current techniques aiming at reducing nephrotoxicity in patients receiving CisPt are still not satisfactory as they can only partially prevent acute kidney injury. New nephroprotective strategies remain to be developed. In the present in vitro study, schizandrin (Schi) and schizandrin B (Schi B), major phytochemicals from Schisandra chinensis (Turcz.) Baill. fruits, were tested on HK‐2 cells along four processes that could help alleviate CisPt toxicity. Results indicated that: (i) both Schi and Schi B enhanced cell survival via reducing apoptosis rate; (ii) only Schi showed moderate effects towards modulation of regeneration capacities of healthy cells; (iii) both Schi and Schi B limited extracellular matrix deposition; and (iv) both compounds could help preventing dedifferentiation processes via the β‐catenin pathway. Schi and Schi B present promising activities for future development of protective agents against CisPt nephrotoxicity. Copyright © 2013 John Wiley & Sons, Ltd.
Cytotoxicity of silica nanoparticles on HaCaT cells
Authors: Liang Hao , Jin Chan , Tang Ying , Wang Fude , Ma Chunwang , Yang Yongji 
Published: October 2013

ABSTRACTDespite the widespread use of silica nanoparticles (SiO2 NPs) in biological and medical fields, their adverse effects have not been clearly elucidated. In this study, spherical SiO2 NPs with a 50 nm diameter were used to study their interaction with HaCaT cells. SiO2 NPs were found to be readily internalized into HaCaT cells and localized in the cytoplasm, lysosomes and autophagosomes. Decreased cell viability and damaged cell membrane integrity showed the cytotoxicity of SiO2 NPs. Significant glutathione depletion and reactive oxygen species generation, which reduced the cellular antioxidant level, could be the major factor of cytotoxicity induced by SiO2 NPs. Copyright © 2013 John Wiley & Sons, Ltd.
Postelectrospinning modifications for alginate nanofiber‐based wound dressings
Authors: Leung Victor , Hartwell Ryan , Elizei Sanam Salimi , Yang Heejae , Ghahary Aziz , Ko Frank 
Published: October 2013

AbstractAlginate nanofibers have been attractive for potential tissue regeneration applications due to a combination of their moisture retention ability and large surface area available in a nonwoven nanofiber form. This study aims to address several challenges in alginate nanofiber application, including the lack of structural stability in aqueous environment and limited cell attachment as compared to commercial wound dressings, via examining crosslinking techniques. In addition to the commonly performed divalent ion crosslinking, a glutaraldehyde double‐crosslinking step and polylysine addition were applied to an electrospun alginate nanofiber nonwoven mat. With optimization of the electrospinning solution, nanofiber morphology was maintained after the two‐stage crosslinking process. Extensibility of the nanofiber mat reduced after the crosslinking process. However, both aqueous stability and cell attachment improved after the postspinning modifications, as shown through degradation tests in phosphate buffered saline solutions and fibroblast cell culture studies, respectively. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 508–515, 2014.
Fabrication of three‐dimensional porous scaffolds with controlled filament orientation and large pore size via an improved E‐jetting technique
Authors: Li Jin Lan , Cai Yan Li , Guo Yi Lin , Fuh Jerry Ying Hsi , Sun Jie , Hong Geok Soon , Lam Ruey Na , Wong Yoke San , Wang Wilson , Tay Bee Yen , Thian Eng San 
Published: October 2013

AbstractBiodegradable polymeric scaffolds have been widely used in tissue engineering as a platform for cell proliferation and subsequent tissue regeneration. Conventional microextrusion methods for three‐dimensional (3D) scaffold fabrication were limited by their low resolution. Electrospinning, a form of electrohydrodynamic (EHD) printing, is an attractive method due to its capability of fabricating high‐resolution scaffolds at the nanometer/micrometer scale level. However, the scaffold was composed of randomly orientated filaments which could not guide the cells in a specific direction. Furthermore, the pores of the electrospun scaffold were small, thus preventing cell infiltration. In this study, an alternative EHD jet printing (E‐jetting) technique has been developed and employed to fabricate 3D polycaprolactone (PCL) scaffolds with desired filament orientation and pore size. The effect of PCL solution concentration was evaluated. Results showed that solidified filaments were achieved at concentration >70% (w/v). Uniform filaments of diameter 20 μm were produced via the E‐jetting technique, and X‐ray diffraction and attenuated total reflectance Fourier transform infrared spectroscopic analyses revealed that there was no physicochemical changes toward PCL. Scaffold with a pore size of 450 μm and porosity level of 92%, was achieved. A preliminary in vitro study illustrated that live chondrocytes were attaching on the outer and inner surfaces of collagen‐coated E‐jetted PCL scaffolds. E‐jetted scaffolds increased chondrocytes extracellular matrix secretion, and newly formed matrices from chondrocytes contributed significantly to the mechanical strength of the scaffolds. All these results suggested that E‐jetting is an alternative scaffold fabrication technique, which has the capability to construct 3D scaffolds with aligned filaments and large pore sizes for tissue engineering applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2014:102B:651–658.
Anodic oxidation of a Co–Ni–Cr–Mo alloy and its inhibitory effect on platelet activation
Authors: Nagai Akiko , Suzuki Yuta , Tsutsumi Yusuke , Nozaki Kosuke , Wada Norio , Katayama Keiichi , Hanawa Takao , Yamashita Kimihiro 
Published: October 2013

AbstractIn this study, surface treatment of a Co–Ni–Cr–Mo alloy (MP35N) was attempted to attain biocompatibility using an anodic oxidation technique. To determine the optimal condition of the anodic oxidation treatment for stent applications, anodic polarization of the alloy was first conducted. After anodic oxidation, the surface topology and wettability were examined, and the composition and chemical states of the surface oxide were characterized. For biocompatibility, stent surfaces must have both cell adhesion and antithrombogenic properties. Therefore, the anodically oxidized surface was assessed with an endothelial cell attachment test and an in vitro platelet adhesion test. The results indicated that the topography, wettability, and composition of the surface oxide film on the alloy were changed by anodic oxidation at a voltage near the passive and transpassive region. The surface roughness and wettability increased after anodic oxidation. The major content of the oxide layer after anodic oxidation was Cr containing a small amount of Mo, and Ni and Co were almost eliminated from the layer. Platelet activation of the alloy decreased significantly after anodic oxidation at an optimal potential, whereas the cytocompatibility remained constant. Therefore, the anodic oxidation is an effective process for treating this alloy for stent applications. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 659–666, 2014.
Size and composition of synthetic calcium sulfate beads influence dissolution and elution rates in vitro
Authors: Roberts Randy , McConoughey Stephen J. , Calhoun Jason H. 
Published: October 2013

AbstractTreatments of osteomyelitis lag behind bacterial resistance to antibiotics. We tested different‐sized calcium sulfate beads and their ability to elute multiple antibiotics in vitro as a possible method to improve the therapeutic delivery in patients. Two sizes of calcium sulfate beads (4.8 and 3.0 mm diameter) that contained vancomycin, tobramycin, or both were dissolved in phosphate‐buffered saline, and the rate of dissolution by weight and antibiotic elution by the disc diffusion assay and high‐pressure liquid chromatography were measured. The 4.8 mm beads showed significantly higher dissolution rates relative to the 3.0 mm beads (2.3 mg/day vs. 1.3 mg/day). While the vancomycin‐loaded 4.8 mm beads eluted for a longer time relative to the 3.0 mm beads (20 days vs. 10 days), the smaller beads had threefold higher elution for the first 2 days, before dropping to near zero elution by day 4. The presence of tobramycin extended the elution of the vancomycin to day 40, which closely matches the recommended 6 weeks to treat orthopedic staphylococcus infections. These data suggest that size and content of the bead are variables that could affect their clinical success, and both could be exploited to tailor treatments of specific infections and injuries. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 667–673, 2014.
Metal release and speciation of released chromium from a biomedical CoCrMo alloy into simulated physiologically relevant solutions
Authors: Hedberg Yolanda , Odnevall Wallinder Inger 
Published: October 2013

AbstractThe objective of this study was to investigate the extent of released Co, Cr(III), Cr(VI), and Mo from a biomedical high‐carbon CoCrMo alloy exposed in phosphate‐buffered saline (PBS), without and with the addition of 10 µMH2O2(PBS + H2O2), and 10 g L−1bovine serum albumin (PBS + BSA) for time periods up to 28 days. Comparative studies were made on AISI 316L for the longest time period. No Cr(VI) release was observed for any of the alloys in either PBS or PBS + H2O2at open‐circuit potential (no applied potential). However, at applied potentials (0.7 V vs. Ag/AgCl), Cr was primarily released as Cr(VI). Co was preferentially released from the CoCrMo alloy at no applied potential. As a consequence, Cr was enriched in the utmost surface oxide reducing the extent of metal release over time. This passivation effect was accelerated in PBS + H2O2. As previously reported for 316L, BSA may also enhance metal release from CoCrMo. However, this was not possible to verify due to the precipitation of metal–protein complexes with reduced metal concentrations in solution as a consequence. This was particularly important for Co–BSA complexes after sufficient time and resulted in an underestimation of metals in solution. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 693–699, 2014.
Development of a rotational cell‐seeding system for tubularized extracellular matrix (ECM) scaffolds in vascular surgery
Authors: Callanan A. , Davis N.F. , McGloughlin T. M. , Walsh M. T. 
Published: October 2013

AbstractTubularized porcine extracellular matrices (ECMs) are under investigation as adjuvant scaffolds for endovascular aneurismal repair (EVAR). Limitations with tubularized ECMs in this setting include difficulties in achieving a confluent endothelium on the scaffold's luminal surface prior to in vivo implantation. In this in vitro study a rotational “cell‐seeding rig” (RCR) was constructed to assess the potential for endothelialization of tubular ECM constructs. Human aortic endothelial cells (HAECs) were cultured onto the luminal surfaces of tubular porcine urinary bladder matrix (UBM) scaffolds and rotated in the RCR at experimental rotational speeds. Results showed that endothelial attachment occurred at a rotation speed of six revolutions per hour. HAECs continued to proliferate after the initial attachment period of 24 h and formed a confluent endothelial monolayer after 14 days of growth. Our results demonstrate that RCRs facilitate attachment of HAECs in vitro at a speed of six revolutions per hour. The endothelialization technique presented in the current study may be important for advancing tissue‐engineering approaches to address some of the current limitations in endovascular treatments of abdominal aortic aneurysms. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 102B: 781–788, 2014.
Compressive Force-Produced CCN2 Induces Osteocyte Apoptosis Through ERK1/2 Pathway
Authors: Hoshi Kenji , Kawaki Harumi , Takahashi Ichiro , Takeshita Nobuo , Seiryu Masahiro , Murshid Sakhr A , Masuda Taisuke , Anada Takahisa , Kato Ryushi , Kitaura Hideki , Suzuki Osamu , Takano-Yamamoto Teruko 
Published: October 2013

ABSTRACT

Osteocytes produce various factors that mediate the onset of bone formation and resorption and play roles in maintaining bone homeostasis and remodeling in response to mechanical stimuli. One such factor, CCN2, is thought to play a significant role in osteocyte responses to mechanical stimuli, but its function in osteocytes is not well understood. Here, we showed that CCN2 induces apoptosis in osteocytes under compressive force loading. Compressive force increased CCN2 gene expression and production, and induced apoptosis in osteocytes. Application of exogenous CCN2 protein induced apoptosis, and a neutralizing CCN2 antibody blocked loading-induced apoptosis. We further examined how CCN2 induces loaded osteocyte apoptosis. In loaded osteocytes, extracellular signal-regulated kinase 1/2 (ERK1/2) was activated, and an ERK1/2 inhibitor blocked loading-induced apoptosis. Furthermore, application of exogenous CCN2 protein caused ERK1/2 activation, and the neutralizing CCN2 antibody inhibited loading-induced ERK1/2 activation. Therefore, this study demonstrated for the first time to our knowledge that enhanced production of CCN2 in osteocytes under compressive force loading induces apoptosis through activation of ERK1/2 pathway. © 2014 American Society for Bone and Mineral Research.
Genomic diversity of low‐risk human papillomavirus genotypes HPV 40, HPV 42, HPV 43, and HPV 44
Authors: Maver Polona J. , Kocjan Boštjan J. , Seme Katja , Poljak Mario 
Published: October 2013

AbstractIn order to investigate the genomic diversity of low‐risk human papillomavirus (HPV) genotypes, a total of 108 isolates of HPV 40, HPV 42, HPV 43, or HPV 44, obtained from anal swabs or tissue specimens of patients with anogenital warts, and cervical swabs of women with cervical intraepithelial neoplasia of different grades, were analyzed. The characterization of genomic variants was established by sequencing one third of the viral genome and analysis of three different genomic regions: L1, LCR, and E6. Maximum variant divergence accounted for 0.4–1.1% of the investigated genomic segments. Several novel, potentially important nucleotide substitutions, deletions, and insertions are described. Altogether, among 14 HPV 40 isolates, a total of nine different genomic variants were identified, composed of eight L1, five LCR, and four E6 genomic variants. Among 49 HPV 42 isolates, a total of 30 genomic variants were identified, composed of 20 L1, 18 LCR, and four E6 genomic variants. Among 10 HPV 43 isolates, distributed into two major genomic variant lineages with clearly defined nucleotide signatures, three genomic variants were identified, composed of three L1, two LCR, and two E6 genomic variants. Among 35 HPV 44 isolates, a total of eight HPV 44 and 11 subtype HPV 44 genomic variants were identified, composed of 13 L1, 14 LCR, and 6 E6 genomic variants. A similar level of genomic diversity of HPV 44 and its subtype was identified in our geographic region as has been reported previously on isolates collected worldwide. J. Med. Virol. 86:272–282, 2014. © 2013 Wiley Periodicals, Inc.
A dynamical energy‐based hysteresis model for iron loss calculation in laminated cores
Authors: Steentjes S. , Henrotte F. , Geuzaine C. , Hameyer K. 
Published: October 2013

SUMMARYThis paper proposes a macroscopic model for ferromagnetic hysteresis that is well‐suited for finite element implementation. The model relies on a consistent thermodynamic formulation. The stored magnetic energy and the dissipated energy are known at all times and not solely after the completion of closed hysteresis loops as is usually the case. Copyright © 2013 John Wiley & Sons, Ltd.
Sea ice concentration variability over the Southern Ocean and its impact on precipitation in southeastern South America
Authors: Saurral Ramiro , Barros Vicente , Camilloni Inés 
Published: October 2013

ABSTRACTThis article quantifies the relationship between sea ice cover (SIC) on the Southern Ocean and precipitation and river discharges over southeastern and eastern South America. The period of analysis covers from 1981 to 2008 and includes monthly mean data of SIC over the Ross, Amundsen–Bellingshausen and Weddell Seas, as well as atmospheric variables and discharges of selected rivers in eastern South America. To isolate the effect of known modes of climate variability on SIC, the signals of El Niño‐Southern Oscillation and the Southern Annual Mode are removed from all time series through regression analysis. Composites of precipitation differences over South America reveal a pattern of enhanced rainfall activity over the South Atlantic Convergence Zone (SACZ) in summer (JFM) when SIC is above average particularly over the Weddell Sea area, while winter (ASO) SIC anomalies show negative and significant correlations with rainfall over much of South America. Moisture fluxes patterns in summer reveal increased moisture transport towards the SACZ region and decreased low‐level jet activity (and precipitation) over northern Argentina. In particular a large dry bias over southeastern South America associated to positive SIC anomalies on the Weddell Sea in September is shown to impact the discharges of the Uruguay and Iguazú Rivers, with the largest effects found two months after the SIC anomaly (November). Although less robust, a relationship with the Paraná River is also found. These results suggest that increased SIC over the Weddell Sea during September can help to forecast drier conditions particularly on the Uruguay basin and somewhat wetter conditions in the SACZ region within the following season.
Metastasectomy for gastrointestinal stromal tumors
Authors: Bamboat Zubin M. , DeMatteo Ronald P. 
Published: October 2013

AbstractGastrointestinal stromal tumor (GIST) is the most common sarcoma of the intestinal tract. Improvements in understanding the molecular pathogenesis of GIST have resulted in novel treatment strategies combining surgery with tyrosine kinase inhibitors (TKIs). Metastasectomy in carefully selected patients who have stable or responsive disease on imatinib should be considered in the multidisciplinary setting. We review existing data on surgical cytoreduction in metastatic GIST while on targeted therapy and compare outcomes with either treatment alone. J. Surg. Oncol. 2014 109:23–27. © 2013 Wiley Periodicals, Inc.
Management of recurrent retroperitoneal sarcoma
Authors: Gyorki David E. , Brennan Murray F. 
Published: October 2013

AbstractApproximately 15% of soft tissue sarcomas are retroperitoneal. The occult location and anatomic complexity results in local recurrences in the majority of patients. Predictors of recurrence include histological subtype, completeness of resection, and the hospital case volume. The most significant predictor of outcome following local recurrence is the resectability of the recurrent disease. An understanding of the implication of tumor biology on outcomes is essential in determining optimal management for patients with recurrent retroperitoneal sarcoma. J. Surg. Oncol. 2014 109:53–59. © 2013 Wiley Periodicals, Inc.
Prognostic information derived from RT‐PCR analysis of peritoneal fluid in gastric cancer patients: Results from a prospective multicenter clinical trial
Authors: Tamura Shigeyuki , Fujiwara Yoshiyuki , Kimura Yutaka , Fujita Junya , Imamura Hiroshi , Kinuta Masakatsu , Yano Masahiko , Hiratsuka Masahiro , Kobayashi Kenji , Okada Kaoru , Mori Masaki , Doki Yuichiro 
Published: October 2013

AbstractObjectiveThe purpose of this study is to define the clinical significance of the molecular analysis of peritoneal lavage fluid in patients with gastric cancer in a multicenter prospective trial.MethodsQuantitative RT‐PCR with CEA and CK‐20 mRNA as target markers was introduced for peritoneal lavage diagnosis in 141 patients with clinically advanced gastric cancer from 9 different institutes. We then evaluated the prognostic factors in patients and also evaluated predictive markers for peritoneal recurrence in 124 patients without peritoneal metastasis at surgeryResultsOut of the 141 cases, 140 patients were successfully analyzed by RT‐PCR of peritoneal lavage fluids. According to multivariate analysis, the combined results of RT‐PCR (CEA and CK‐20) and CK‐20 alone in addition to pathological N (pN)‐stage were significantly correlated with overall survival. Multivariate analysis showed that the RT‐PCR (CEA and CK‐20) and CEA alone in addition to pathological T‐stage, pN‐stage, and histological grade were significantly correlated with peritoneal recurrence after surgery.ConclusionThis is the first multicenter prospective study to show that RT‐PCR analysis of peritoneal lavage fluid with the combination of CEA and CK‐20 markers was useful for predicting overall survival and peritoneal recurrence in patients with clinically advanced gastric cancer. J. Surg. Oncol. 2014 109:75–80. © 2013 Wiley Periodicals, Inc.
Measurement of the Rate of Hydrogen Peroxide Thermal Decomposition in a Shock Tube Using Quantum Cascade Laser Absorption Near 7.7 μm
Authors: Sajid M. B. , Es‐sebbar Et. , Javed T. , Fittschen C. , Farooq A. 
Published: October 2013

ABSTRACTHydrogen peroxide (H2O2) is formed during hydrocarbon combustion and controls the system reactivity under intermediate temperature conditions. Here, we measured the rate of hydrogen peroxide decomposition behind reflected shock waves using midinfrared absorption of H2O2 near 7.7 µm. We performed the experiments in diluted H2O2/Ar mixtures between 930 and 1235 K and at three different pressures (1, 2, and 10 atm). Under these conditions, the decay of hydrogen peroxide is sensitive only to the decomposition reaction rate, H2O2 + M → 2OH + M (k1). The second‐order rate coefficient at low pressures (1 and 2 atm) did not exhibit any pressure dependence, suggesting that the reaction was in the low‐pressure limit. The rate data measured at 10 atm exhibited falloff behavior. The measured decomposition rates can be expressed in Arrhenius forms as follows:

urn:x-wiley:05388066:kin20827:equation:kin20827-math-0001
urn:x-wiley:05388066:kin20827:equation:kin20827-math-0002
Assessment of aspiration risk from dynamic modulation of endotracheal tube cuff pressure
Authors: Kanotra Sohit P. , Propst Evan J. , Luginbuehl Igor , Campisi Paolo , Fisher Joseph A. , Forte Vito 
Published: October 2013

Objectives/HypothesisTo assess the risk of aspiration using a novel valve circuit that dynamically modulates endotracheal tube cuff pressure during the ventilatory cycle using bench and live animal models.Study DesignAnimal model.MethodsThe bench model consisted of a cuffed endotracheal tube inserted into an artificial trachea. Leakage of liquid around the cuff was measured after 4 hours of constant or dynamic modulation of cuff pressure at variable peak end expiratory pressures. In the porcine model, eight animals were ventilated with the modulating valve circuit and compared to eight controls ventilated with a constant cuff pressure (25 cm of water). Aspiration was monitored quantitatively using a pH probe (measured as voltage) and visually using fluoroscopy.ResultsThere was no difference in the amount of fluid leakage around the endotracheal tube cuff in the constant or dynamically modulated pressure‐cuff groups in the bench or animal models.ConclusionDynamically modulating endotracheal tube‐cuff pressures to minimize tracheal mucosal damage does not increase the tendency to leak around the cuff compared to endotracheal tube cuffs inflated to a constant pressure.Level of EvidenceN/A. Laryngoscope, 124:1415–1419, 2014