bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mucosal melanoma: A series of seven cases and review of literature
Authors: Aashita  , Yadav Vikas , Sharma Rajiv , Mishra Hemendra , Choudhury Apratim R. , Thakur Pragyat 
Published: August 2024

ABSTRACT

Background:
Mucosal melanoma (MM) is the uncontrolled proliferation of melanocytes originating from the mucosal surface. MM is rare and comprises 0.3% of all cancers.

Methods:
We retrospectively analyzed records of 6582 cases who reported to our center from June 1, 2017 to May 31, 2022. Only seven cases of mucosal melanoma were found among them.

Results:
In this article, we have reported seven cases of mucosal melanoma that presented over a period of 5 years. A comparison of mucosal and cutaneous melanoma has been done.

Conclusion:
MM is an aggressive tumor with a dismal prognosis. Surgery, chemotherapy, radiation, targeted and immunotherapy are various treatment options; however, prognosis remains less favorable as timely diagnosis is difficult.
The role of GADD45G methylation in endometrial cancer: Insights into CDK1/CCNB1 activation and therapeutic opportunities
Authors: Wang Chunxiao , Shan Shuzhi , Li Xinjun , Wang Huifang , Qi Jie , Zhao Sufen 
Published: August 2024

ABSTRACT

Introduction:
Accumulating evidence suggests the significant involvement of GADD45G in the development of various cancers. This study investigates GADD45G’s involvement and methylation status in endometrial cancer (EC), along with molecular mechanisms and potential therapies.

Methods:
The expression of GADD45G in EC tissues and controls was evaluated using RNA-seq, quantitative real-time polymerase chain reaction (qRT-PCR), and western blotting (WB). Methylation-specific PCR (MSP) evaluated GADD45G’s methylation status. Protein-protein interaction (PPI) prediction identified potential interactors of GADD45G, and co-immunoprecipitation (co-IP) confirmed GADD45G interact with Cyclin-dependent kinase 1 (CDK1) and cyclin B1 (CCNB1). Several cell behavior assays were conducted in both in vitro and in vivo settings to comprehensively understand the impact of GADD45G dysregulation in EC.

Results:
Our findings revealed a significant decrease in the expression of GADD45G in endometrial cancer tissues and cells, which was attributed to its methylation status. Reduced GADD45G expression correlated with increased invasive behaviors in EC cells. Furthermore, GADD45G negatively regulated CDK1 and CCNB1, promoting invasive behaviors at transcript and protein levels.

Conclusion:
This study demonstrated that the downregulation of GADD45G, mediated by methylation, facilitates the invasive behaviors of EC cells through interaction with the CDK1/CCNB1. These findings enhance understanding of the molecular mechanisms underlying endometrial cancer and suggest potential therapeutic strategies targeting GADD45G for treatment.
Tandem mass tag-based quantitative proteomic analysis of metformin’s inhibitory effects on ovarian cancer cells
Authors: Wang Dongyue , Wang Jingchen , Cui Yingying 
Published: August 2024

ABSTRACT

Purpose:
Metformin (MET), a type 2 diabetes treatment, has attracted increased attention for its potential antitumor properties; however, the precise mechanism underlying this activity remains unclear. Our previous in vivo and in vitro studies revealed MET’s inhibitory effect on ovarian cancer, with the synergistic effects of MET and the MDM2 inhibitor RG7388 contributing to ovarian cancer treatment. This study further explores the mechanism underlying MET’s inhibition of ovarian cancer.

Materials and Methods:
Following MET treatment, we analyzed the differentially expressed proteins in ovarian cancer cells using a tandem mass tag (TMT)-based proteomic approach coupled with bioinformatics.

Results:
Using A2780 and SKOV3 ovarian cancer cells, we identified six upregulated and two downregulated proteins after MET treatment. Bioinformatics analysis revealed that these proteins predominately affect ovarian cancer cells by regulating iron ion transport, iron ion homeostasis, and mitochondrial and ribosomal functions. Validation via western blot confirmed MET-induced elevation of hydroxybutyrate dehydrogenase type 2 (BDH2) protein expression levels in A2780 and SKOV3 cells.

Conclusions:
Overall, our findings suggest that combining MET with other metabolic drugs, such as iron-chelating agents and mitochondrial inhibitors, may result in synergistic antitumor effects, thereby offering novel avenues for ovarian cancer treatment development.
Lymph node metastasis in grossly apparent early-stage epithelial ovarian cancer: A retrospective clinical study at a tertiary institute
Authors: Zhu Menghan , Li Jun , Lu Lijuan , Duan Jie , Jiang Wei 
Published: August 2024

ABSTRACT

Objective:
This study aimed to evaluate the incidence and predict the risk factors of lymph node (LN) metastasis among patients with grossly apparent early-stage epithelial ovarian cancer (EOC).

Methods:
We retrospectively reviewed the clinicopathologic data and follow-up information of 266 patients who underwent LN dissection for apparent early-stage EOC between January 2018 and September 2022 at the Obstetrics and Gynecology Hospital of Fudan University.

Results:
Among 266 patients, 44 (16.5%) showed LN metastasis, of which 65.9% and 59.1% presented in the pelvic region and para-aortic region, respectively. Univariate analysis revealed higher LN positivity in patients with high-grade serous carcinoma (HGSC), preoperative imaging suggestive of LN metastasis, bilateral adnexal involvement, lymphovascular space invasion (LVSI), positive peritoneal cytology, and clinical stage IIA. LN metastases were identified in 7.9%, 10.2%, and 39.7% of clinical stage IA/B, IC, and IIA disease cases, respectively. Multivariate analysis confirmed significantly higher LN positivity rates in patients with HGSC, LVSI, and clinical stage IIA. In clinical stage IIA EOC, the 3-year progression-free survival (PFS) rates were 65.8% and 77.4% (P = 0.360) for LN-negative and LN-positive groups, respectively. In clinical stage I EOC, the 3-year PFS rates were 93.5% and 59.4% (P < 0.001) for LN-negative and LN-positive groups, respectively.

Conclusions:
High-grade serous histology, LVSI, and clinical stage IIA disease are predictive factors for LN involvement in early-stage EOC. In addition, LN metastasis appears to be associated with worse PFS in clinical stage I EOC compared with clinical stage IIA EOC.
Investigating the accuracy of machine vision and augmented reality in percutaneous computed tomography-guided interventions: A phantom study
Authors: Huang Bingyu , Wei Yizhi , Zhang Bing , Chen Jin , Guo Rui , Zhou Steven Zhiying , Lin Zhigang , Lin Zhengyu 
Published: August 2024

ABSTRACTObjectives:This study aimed to evaluate the accuracy of percutaneous computed tomography (CT)-guided puncture based on machine vision and augmented reality in a phantom.Materials and Methods:The surgical space coordinate system was established, and accurate registration was ensured using the hierarchical optimization framework. Machine vision tracking and augmented reality display technologies were used for puncture navigation. CT was performed on a phantom, and puncture paths with three different lengths were planned from the surface of the phantom to the metal ball. Puncture accuracy was evaluated by measuring the target positioning error (TPE), lateral error (LE), angular error (AE), and first success rate (FSR) based on the obtained CT images.Results:A highly qualified attending interventional physician performed a total of 30 punctures using puncture navigation. For the short distance (4.5–5.5 cm), the TPE, LE, AE, and FSR were 1.90 ± 0.62 mm, 1.23 ± 0.70 mm, 1.39 ± 0.86°, and 60%, respectively. For the medium distance (9.5–10.5 cm), the TPE, LE, AE, and FSR were 2.35 ± 0.95 mm, 2.00 ± 1.07 mm, 1.20 ± 0.62°, and 40%, respectively. For the long distance (14.5–15.5 cm), the TPE, LE, AE, and FSR were 2.81 ± 1.17 mm, 2.33 ± 1.34 mm, 0.99 ± 0.55°, and 30%, respectively.Conclusion:The augmented reality and machine vision-based CT-guided puncture navigation system allows for precise punctures in a phantom. Further studies are needed to explore its clinical applicability.
Chinese guidelines for integrated diagnosis and treatment of intestinal microecology technologies in tumor application (2024 Edition)
Authors: Wang Qiang , He Mingxin , Liang Jing , Tan Xiaohua , Wu Qingming , Wang Jun , Li Xiaoan , Qiao Mingqiang , Huang Ziming , Xie Qi , Liu Zhe , Ren Hua , Wang Liang , Zhou Hao , Shao Liang , Shu Rong , Wu Wei , Yang Wenyan , Wang Hua , Sun Zhiqiang , Xu Xiaojun , Zhang Xingding , Li Zhiming , Zhang Yu , Meng Jingye , Zhu Yanli , Chen Feng , Qu Rong , Chen Peng , Li Shuluan , Shi Yuanyuan , Mao Xin , Hu Bichuan , Zhang Yukui , Cao Yu J. , Guo Zhi ,   The Tumor and Microecology Professional Committee of the Chinese Anti-cancer Association
Published: August 2024

ABSTRACT
Intestinal microecology (IM) is the largest and most important microecological system of the human body. Furthermore, it is the key factor for activating and maintaining the physiological functions of the intestine. Numerous studies have investigated the effects of the gut microbiota on the different tissues and organs of the human body as well as their association with various diseases, and the findings are gradually being translated into clinical practice. The gut microbiota affects the occurrence, progression, treatment response, and toxic side effects of tumors. The deepening of research related to IM and tumors has opened a new chapter in IM research driven by methods and technologies such as second-generation sequencing and bioinformatics. The IM maintains the function of the host immune system and plays a pivotal role in tumor-control drug therapy. Increasing evidence has proven that the efficacy of tumor-control drugs largely depends on the IM balance, and strategies based on the IM technology show promising application prospects in the diagnosis and treatment of tumor. The Tumor and Microecology Professional Committee of the Chinese Anti-cancer Association gathered relevant experts to discuss and propose the “Chinese guidelines for integrated diagnosis and treatment of IM technologies in tumor application (2024 Edition),” which was established based on the research progress of the application of the IM technology in tumor to provide a basis for the standardization of the diagnosis and treatment of the IM technology in the tumor.
Evaluation of robotic-assisted navigation system for CT-guided thoracic and abdominal lesion puncture: A prospective clinical study
Authors: Jing Yifan , Zhang Jian , Jin Yong , Bai Xuming 
Published: August 2024

ABSTRACT

Introduction:
The study aims to compare the accuracy and safety of robotic-assisted navigation puncture to freehand puncture during computed tomography (CT)-guided percutaneous needle insertion in the chest and abdomen.

Methods:
A total of 60 patients required percutaneous puncture procedures, with 40 involving the chest and 20 involving the abdomen. Eligible patients were randomly assigned to two groups. The test group punctured using a robotic-assisted navigation system, whereas the control group punctured manually. The primary outcome assessment standards are single puncture success rates, with the number of needle modifications and CT scan timings during the procedure serving as supplementary outcome evaluation standards. The Wilcoxon rank sum test is used for the comparison.

Results:
The puncture procedure’s success rates after just one puncture: The test group punctures accurately without adjusting the puncture needle, while the control group uses an average number of 1.73 ± 1.20 pins. The once-puncture success rate of robot navigation puncture is considerably higher than that of bare-handed puncture (P < 0.001). The times of CT scan are necessitated when the puncture is in place: the average times in the test group is 3.03 ± 0.18 times, while the control group is 4.70 ± 1.24 times.

Conclusion:
In conclusion, the robotic-assisted navigation system improves puncture accuracy while reducing the need for needle corrections during percutaneous puncture procedures. It also shortens CT scans and reduces radiation exposure from X-rays.
PADI3 inhibits epithelial–mesenchymal transition by targeting CKS1-induced signal transduction in colon cancer
Authors: Chai Zhengbin , Zhu Changhui , Wang Xiwei , Zheng Yingying , Han Fabin , Xie Qi , Liu Chunyan 
Published: August 2024

ABSTRACT

Background:
Protein arginine deiminase 3 (PADI3) is involved in various biological processes of human disease. PADI3 has recently received increasing attention due to its role in tumorigenesis. In a previous study, we found that PADI3 plays a tumor suppressor role in colon cancer by inducing cell cycle arrest, but its critical role and mechanism in cancer metastasis remain obscure. In this study, we fully studied the role of PADI3 in colon cancer cell metastasis.

Methods:
The expression levels of related proteins were detected by Western blotting, and Transwell and wound healing assays were used to examine the cell migration ability. Flow cytometry was used to measure and exclude cell apoptosis-affected cell migration. Both overexpression and rescue experiments were employed to elucidate the molecular mechanism of CKS1 in colon cancer cells.

Results:
The expression levels of PADI3 and CKS1 are negatively related, and PADI3 can promote CKS1 degradation in a ubiquitin-dependent manner. PADI3 can suppress colon cancer cell migration and reduce the wound healing speed by inhibiting CKS1 expression. The molecular mechanism showed that CKS1 can promote EMT by increasing Snail and N-cadherin expression and suppressing E-cadherin expression. PADI3, as a suppressor of CKS1, can block the process of EMT by impairing CKS1-induced Snail upregulation and E-cadherin downregulation; however, the expression of N-cadherin cannot be rescued.

Conclusions:
CKS1 promotes EMT in colon cancer by regulating Snail/E-cadherin expression, and this effect can be reversed by PADI3 via the promotion of CKS1 degradation in a ubiquitylation-dependent manner.
Expert consensus on the multidisciplinary diagnosis and treatment of multiple ground glass nodule-like lung cancer (2024 Edition) ∆
Authors: Liu Baodong , Ye Xin , Fan Weijun , Zhi Xiuyi , Ma Haitao , Wang Jun , Wang Peng , Wang Zhongmin , Wang Hongwu , Wang Xiaoping , Niu Lizhi , Fang Yong , Gu Shanzhi , Lu Qiang , Tian Hui , Zhu Yulong , Qiao Guibin , Zhong Lou , Wei Zhigang , Zhuang Yiping , Liu Hongxu , Liu Lingxiao , Liu Lei , Chi Jiachang , Sun Qing , Sun Jiayuan , Sun Xichao , Yang Nuo , Mu Juwei , Li Yuliang , Li Chengli , Li Chunhai , Li Xiaoguang , Li Kang’an , Yang Po , Yang Xia , Yang Fan , Yang Wuwei , Xiao Yueyong , Zhang Chao , Zhang Kaixian , Zhang Lanjun , Zhang Chunfang , Zhang Linyou , Zhang Yi , Chen Shilin , Chen Jun , Chen Kezhong , Chen Weisheng , Chen Liang , Chen Haiquan , Fan Jiang , Lin Zhengyu , Lin Dianjie , Xian Lei , Meng Zhiqiang , Zhao Xiaojing , Hu Jian , Hu Hongtao , Liu Chen , Liu Cheng , Zhong Wenzhao , Yu Xinshuang , Jiang Gening , Jiao Wenjie , Yao Weirong , Yao Feng , Gu Chundong , Xu Dong , Xu Quan , Ling Dongjin , Tang Zhe , Huang Yong , Huang Guanghui , Peng Zhongmin , Dong Liang , Jiang Lei , Jiang Junhong , Cheng Zhaoping , Cheng Zhigang , Zeng Qingshi , Jin Yong , Lei Guangyan , Liao Yongde , Tan Qunyou , Zhai Bo , Li Hailiang 
Published: August 2024

ABSTRACT
This expert consensus reviews current literature and provides clinical practice guidelines for the diagnosis and treatment of multiple ground glass nodule-like lung cancer. The main contents of this review include the following: ① follow-up strategies, ② differential diagnosis, ③ diagnosis and staging, ④ treatment methods, and ⑤ post-treatment follow-up.
Guidelines for power and time variables for microwave ablation in porcine lung in vitro
Authors: Cai Hongchao , Shan Guanglian , Wei Zhigang , Zhao Wenhua , Xue Guoliang , Zhang Chao , Ye Xin 
Published: August 2024

ABSTRACT

Purpose:
Determination of the appropriate ablative parameters is the key to the success and safety of microwave ablation (MWA) of lung tumors. The purpose of this study was to provide guidelines and recommendations for the optimal time and power for lung tumor MWA.

Material and Methods:
MWA using a 2450-MHz system was evaluated in a porcine lung. The independent variables were power (30, 40, 50, 60, 70, and 80 W) and time (2, 4, 6, 8, 10, and 12 min), and the outcome variable was the volume of ablation. Lung tissues were procured after MWA for measurement and histological evaluation. Analysis of variance was used for statistical analysis, followed by least significant difference (LSD) t-tests where appropriate. A P value of <0.05 was considered statistically significant.

Results:
The outcome variable (ablative volume) was significantly affected by time, power, and time/power interaction (P < 0.05). When the total output energy was kept constant, the combination of higher power and shorter time obtained a larger ablative volume, especially in the low- and medium-energy groups (P < 0.01).

Conclusions:
We propose guidelines for ablative volume based on different time and power variables to provide a reference for clinical applications.
Surgery or radiotherapy improves survival in elderly patients with early non-small cell lung cancer: A population-based analysis
Authors: Shi Pingfan , Li Ziye , Zhang Yanfei , Shen Changxing , Xia Qing , Cao Chuanwu , Li Ming , Fan Lihong 
Published: August 2024

ABSTRACT

Objective:
There is a lack of evidence to support a consensus on whether surgery or radiotherapy is optimal for elderly or very elderly patients with early-stage non-small cell lung cancer (NSCLC). We aimed to assess the impact of surgery or radiotherapy on survival in elderly (≥70 years) and very elderly (≥80 years) patients with early-stage NSCLC.

Methods:
Patients aged ≥70 years diagnosed with early-stage NSCLC between January 1, 1975, and December 31, 2018, were identified in the Surveillance, Epidemiology, and End Results (SEER) database. Overall survival (OS) and cancer-specific survival (CSS) were assessed based on surgery, radiotherapy, and no-treatment groups.

Results:
Data for 15,224 NSCLC patients aged ≥70 years were collected, which consisted of 6949 (45.6%) patients who underwent surgery alone, 5014 (32.9%) who underwent radiotherapy alone, and 3261 (21.5%) who received no treatment. Surgery significantly improved patient survival compared with no treatment (MST: 74 months vs. 7 months, HR: 0.201, 95% CI: 0.186–0.217, P < 0.001), as did radiotherapy (MST: 28 months vs. 7 months, HR: 0.440; 95% CI: 0.413–0.469, P < 0.001). Surgery also resulted in improved survival compared with radiotherapy (74 months vs. 28 months, HR: 0.455; 95% CI: 0.430–0.482, P < 0.001). A similar conclusion was made from the analysis of CSS. A subgroup analysis further confirmed the survival benefits.

Conclusions:
The results of this large-scale retrospective study indicate that both surgery and radiotherapy significantly enhance survival outcomes in patients aged ≥70 or ≥80 years with early-stage NSCLC. The survival benefits of surgery were particularly notable.
Morphological Variations of the Liver and Its Applied Significance
Authors: Sangeetha A. , Kumar S. Nandha 
Published: August 2024

Background:
Morphological features of the liver serve as important landmarks for radiologists and surgeons in the diagnosis and interpretations of liver diseases. Accessory fissures and accessory lobes often mislead the clinicians in the diagnosis of liver pathologies.

Aim:
This study is aimed to determine the morphological variations of the liver and implicate its clinical significance.

Materials and Methods:
The present study was conducted in the department of anatomy, with 50 formalin-fixed livers obtained from the cadavers of both sexes. Morphological surface features were observed, photographed, and studied.

Results:
About 22 (44%) livers showed variations such as accessory fissures (24%) present in the right, left, caudate, and quadrate lobe. Diaphragmatic fissures or grooves were seen in 10 (20%). Pons hepatis was seen in 3 (6%) livers. Riedels lobe was seen in 4 (8%), and the absence of quadrate lobe was seen in 1 (2%) specimen.

Conclusion:
The knowledge of abnormality in surface features of the liver is clinically significant and will help the radiologists and surgeons in the interpretation of normal and abnormal liver.
A Study to Assess the Cognitive Functions and the General Health in Patients with Substance Dependence
Authors: Bhusri Leezu , Bathla Manish , Gupta Parul , Singh Angad Harshbir 
Published: August 2024

Introduction:
Cognitive function has been addressed as a hallmark feature of substance use disorders, with alteration in “executive” domains of attention, inhibition/regulation, working memory, and decision-making. Poor cognition or cognitive dysfunction has been recognized as a fundamental impairment in addiction and a potentially important target for intervention. The present study was planned with the aim to assess the cognitive functions and general health in patients with substance dependence and also to correlate general health with the level of cognitive function.

Methodology:
Ninety participants were included in the study and were categorized into three groups of thirty each as patients taking alcohol, opioids, and nicotine. Consecutive sampling was adopted for the sample collection. Each participant was assessed for dependence, demographic data, and tested by the Addenbrooke's Cognitive Examination Scale-III and General Health Questionnaire-12. Descriptive statistics were applied to calculate the frequencies, percentage, mean, median, and standard deviation. Data were further analyzed for significance using the Chi-square, correlation, and Mann–Whitney test.

Results:
There is a statistically significant difference seen in marital status (P = 0.005), education (P = 0.001), family type (P = 0.008), and locality (P = 0.000). Furthermore, with substance use, there is a significant change in the domains of general health with P < 0.001, and there is a significant decline in sustaining attention with P = 0.007.

Conclusion:
Thus, we conclude that substance use has a significant negative impact on cognition mainly attention and working memory with the impact on general health as well.
Self-Reported Morbidities Among Software Professionals – A Cross-Sectional Study
Authors: Patnaik Lipilekha , Pattanaik Sumitra , Sahu Trilochan 
Published: August 2024

Background:
In the modern era, persons working in software companies are increasing, and there is an emergence of computer-related health problems.

Objectives:
The aim of this study is to estimate the prevalence of various morbidities among software professionals and to assess the factors associated with these problems.

Materials and Methods:
A cross-sectional study of 4 months from March 2016 to June 2016 was conducted among software professionals. Two hundred and sixty software professionals in the current job for the past 1 year were included in the study. A Google form was created through Google drive of the primary investigator. The E-mail id of persons working in software companies was collected through friends, relatives, and Internet. One person can send one response option was selected. The Google form was sent to their mail id with a request to forward the Google form to their contacts working in software.

Results:
Among the study participants, 53.8% of persons feel they were having health problems due to prolonged computer use. More than 70% had visual problems such as eye pain, eye strain, eye redness, blurring of vision, or watery eyes. It was observed, 63% of software professionals had some type of musculoskeletal problems. About 40% had headaches frequently and 47% had gastrointestinal problems. About 67.7% feel stressed due to their prolonged work and 35% feel depressed. A significant association was found between participants with visual and musculoskeletal complaints using antiglare screen and soft keypads and those not using, respectively.

Conclusion:
Ocular discomfort, musculoskeletal disorders, and psychosocial problems were main health problems among constant computer users which needs urgent intervention.