bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human saphenous vein endothelial cell adhesion and expansion on micropatterned polytetrafluoroethylene
Authors: Boivin Marie‐Claude , Chevallier Pascale , Hoesli Corinne A. , Lagueux Jean , Bareille Reine , Rémy Murielle , Bordenave Laurence , Durrieu Marie‐Christine , Laroche Gaétan 
Published: September 2012

AbstractIntimal hyperplasia and thrombosis are responsible for the poor patency rates of small‐diameter vascular grafts. These complications could be avoided by a rapid and strong adhesion of endothelial cells to the prosthetic surfaces, which typically consist of expanded polytetrafluoroethylene (PTFE) for small‐diameter vessels. We have previously described two peptide micropatterning strategies that increase the endothelialization rates of PTFE. The micropatterns were generated either by inkjet printing 300 μm squares or by spraying 10.1 ± 0.1 μm diameter droplets of the CGRGDS cell adhesion peptide, while the remaining surface was functionalized using the CWQPPRARI cell migration peptide. We now directly compare these two micropatterning strategies and examine the effect of hydrodynamic stress on human saphenous vein endothelial cells grown on the patterned surfaces. No significant differences in cell adhesion were observed between the two micropatterning methods. When compared to unpatterned surfaces treated with a uniform mixture of the two peptides, the cell expansion was significantly higher on sprayed or printed surfaces after 9 days of static cell culture. In addition, after 6 h of exposure to hydrodynamic stress, the cell retention and cell cytoskeleton reorganization on the patterned surfaces was improved when compared to untreated or random treated surfaces. These results indicate that micropatterned surfaces lead to improved rates of PTFE endothelialization with higher resistance to hydrodynamic stress. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 694–
703, 2013.
The roles of PI3K/Akt signaling pathway in regulating MC3T3‐E1 preosteoblast proliferation and differentiation on SLA and SLActive titanium surfaces
Authors: Gu Ying‐Xin , Du Juan , Si Mi‐Si , Mo Jia‐Ji , Qiao Shi‐Chong , Lai Hong‐Chang 
Published: September 2012

AbstractChemical modification to produce a hydrophilic microrough titanium (Ti) implant surface has been shown to increase osseointegration compared with microrough topography alone. This study aimed to investigate the roles of PI3K/Akt signaling pathway in regulating proliferation and differentiation of osteoblasts in response to surface microroughness and hydrophilicity. Ti disks were manufactured to present different surface morphologies: a smooth pretreatment surface (PT), a rough hydrophobic surface that was sand‐blasted, large‐grit, acid‐etched (SLA), and an SLA surface with the same roughness that was chemically modified to possess high wettability/hydrophilicity (SLActive/modSLA). MC3T3‐E1 cells were cultured on these substrates with or without LY294002, a PI3K inhibitor, and their behaviors, including cell viability (MTT colorimetric assay), alkaline phosphatase (ALP) activity, and osteogenic genes expression of osteopontin (OPN) and osteocalcin (OCN) were measured. Western blot was applied to detect the expression of PI3K/Akt signal pathway proteins. The results showed that a decrease in osteoblast proliferation associated with the Ti surfaces (SLActive > SLA > PT) correlated with an increase in activity of the osteogenic differentiation markers ALP. The peak of ALP activity appeared earlier at 7 days for the SLActive surfaces compared with the SLA and PT surfaces. Osteoblast proliferation, as well as the level of p‐Akt, was significantly inhibited by LY294002 in all three Ti surfaces. The top value of ALP activity was increased with the inhibition of PI3K/Akt signaling pathway while the time of the peak appeared was not advanced. The expression levels of OPN and OCN were upregulated by the effect of surface roughness and hydrophilicity, which were further enhanced by LY294002. In conclusion, osteogenic responses to SLActive surface were moderately better than the SLA surface and protein expression studies indicated that PI3K/Akt signaling activation may be responsible for this increased osteogenic differentiation. Surface microroughness and hydrophilicity may affect osteoblast functions by targeting osteoblast proliferation and the early stage of osteoblast differentiation through PI3K/Akt signaling pathway. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 748–754, 2013.
Subscapularis tendon tears: A common sonographic finding in symptomatic postarthroplasty shoulders
Authors: Ives Elizabeth P. , Nazarian Levon N. , Parker Laurence , Garrigues Grant E. , Williams Gerald R. 
Published: September 2012

AbstractPurpose.Subscapularis tendon tears can complicate shoulder arthroplasty because the subscapularis tendon is typically divided surgically to gain access to the joint. The purpose of this study is to document the prevalence of subscapularis tears on sonography of symptomatic postarthroplasty shoulders.Methods.We retrospectively reviewed official reports of shoulder sonograms performed over an 11‐year period by one experienced radiologist. Sonographic findings in 112 (mean age, 61.7 years; age range, 38–87 years; 40% female) symptomatic patients who had undergone either total shoulder arthroplasty or hemiarthroplasty were compared with findings in both a symptomatic control group consisting of 209 patients (mean age, 55.2 years; age range, 32–79 years; 37% female) who had undergone surgery for rotator cuff repair but no arthroplasty, and an asymptomatic control group consisting of 11 (mean age, 66 years; age range, 61–77 years; 45% female) volunteers who had shoulder arthroplasty but were without complaints.Results.The prevalence of subscapularis tears in arthroplasty patients (51%, 57/112) was higher (p < 0.0001) than that of rotator cuff repair patients (16%, 33/209). In the asymptomatic volunteers, subscapularis tears (9%, 1/11) were less common (p < 0.01) than in the symptomatic patients.Conclusions.Subscapularis tendon tears are a common sonographic finding in symptomatic postarthroplasty shoulders. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2013.
Subclinical left and right ventricular systolic dysfunction in behcet's disease: A combined tissue doppler and velocity vector imaging study
Authors: Yurdakul Selen , Erdemir Vefa Aslı , Tayyareci Yelda , Yildirimturk Ozlem , Salih Gurel Mehmet , Aytekin Saide 
Published: September 2012

BackgroundBehcet's disease (BD) is a chronic inflammatory disease characterized by recurrent oral and genital ulcerations and ocular lesions. Subclinical cardiac involvement may develop in BD patients. We aimed to evaluate subclinical left ventricular (LV) and right ventricular (RV) systolic dysfunction in BD patients without any apparent cardiovascular disease.MethodsWe studied 50 BD patients (43.8 ± 9.7 years, 59% men) and 30 healthy controls (45.4 ± 8.2 years, 60% men). Conventional echocardiography, tissue Doppler imaging, and velocity vector imaging‐based strain measurements were performed to analyze LV and RV systolic functions.ResultsLV isovolumic myocardial acceleration, peak systolic velocity during isovolumic contraction (isovolumic contraction velocity), were significantly lower, while myocardial performance index was increased in BD patients. RV peak systolic velocity, isovolumic myocardial acceleration, and isovolumic contraction velocity were also markedly lower in BD patients. LV and RV longitudinal peak systolic strain and strain rate were significantly lower in patients than in controls, demonstrating subclinical ventricular systolic dysfunction.ConclusionsVentricular long‐axis functions are important markers of myocardial contractility. Novel echocardiographic techniques may provide additional data for detecting early deterioration in ventricular systolic function in patients with BD. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound 41:347–353, 2013
High‐intensity focused ultrasound combined with herpes simplex virus thymidine kinase gene‐loaded ultrasound‐targeted microbubbles improved the survival of rabbits with VX2 liver tumor
Authors: Zhou Shi‐Ji , Li Sheng‐Wei , Wang Ji‐Jian , Liu Zuo‐Jin , Yin Guo‐Bing , Gong Jian‐Ping , Liu Chang‐An 
Published: September 2012

AbstractBackgroundTo explore the anti‐tumor effect of high‐intensity focused ultrasound (HIFU) combined with herpes simplex virus thymidine kinase (HSV‐TK) gene‐loaded ultrasound‐targeted microbubbles on VX2 rabbit liver tumors.MethodsSeventy‐five New Zealand white rabbits were randomly divided into five groups after the models of VX2 rabbit liver tumors were established: (a) HIFU group; (b) HIFU and HSV‐TK group (HIFU + HSV‐TK); (c) HIFU, HSV‐TK and ultrasound group (HIFU + HSV‐TK + US); (d) HIFU, HSV‐TK gene‐loaded microbubbles and ultrasound group (HIFU + HSV‐TK‐MBs + US); and (e) HSV‐TK gene‐loaded microbubbles and ultrasound group (HSV‐TK‐MBs + US). After 2 weeks of VX2 liver tumor implantation, rabbits in groups (a), (b), (c) and (d) received HIFU to establish rabbit models of residual tumor by ablating 80% of the tumor volume. After HIFU ablation, rabbits in different groups received MBs wrapped around HSV‐TK or HSV‐TK solution via marginal ear veins and/or local ultrasonic irradiation to the tumor. Six rabbits in each group were sacrificed 48 h after the corresponding treatment, and tumors were extracted for in vitro experiments. Thymidine kinase mRNA was detected by the real‐time polymerase chain reaction. The green fluorescent protein expression in liver tumor was detected by western blotting and immunohistochemistry. Tumor cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling. The growth curves of VX2 liver tumors and survival curves of rabbits were compared.ResultsForty‐eight hours after treatment, TK mRNA and protein were the highest in the HIFU + HSV‐TK + US + MBs group and the HSV‐TK + US + MBs group (p < 0.05). At 48 h after treatment, the apoptotic index of tumor cells in HIFU + HSV‐TK‐MBs + US group was the highest (p < 0.05). Compared to other groups, HIFU combined with MBs wrapped HSV‐TK suicide gene significantly inhibited tumor growth in vivo (p < 0.05) and prolonged the survival time of animals (p < 0.05).ConclusionsHIFU combined with HSV‐TK gene‐loaded ultrasound‐targeted MBs significantly inhibited the growth of VX2 rabbit liver tumors in vivo and prolonged the survival time of the animals, providing a novel gene delivery method and a novel strategy for liver tumor treatment. Copyright © 2012 John Wiley & Sons, Ltd.
Use of conventional MR imaging and diffusion‐weighted imaging for evaluating the risk grade of gastrointestinal stromal tumors
Authors: Zhou Hai Ying , Zhang Xiao Ming , Zeng Nan Lin , Jian Shun Hai , Tang Wei 
Published: September 2012

AbstractPurpose:To study the risk grade of gastrointestinal stromal tumors (GISTs) with conventional MR imaging and diffusion‐weighted imaging (DWI).Materials and Methods:The abdominal MR images with DWI of 23 patients with pathologically proven GISTs during January 2010 to May 2011 were retrospectively reviewed. The conventional MR imaging findings and apparent diffusion coefficient (ADC) values of the tumors related to the risk grade were analyzed.Results:In the 23 patients, there were 13 patients with high‐risk, 5 with medium‐risk, 5 with low‐risk, and 0 with very low‐risk GISTs. Most of the conventional MR findings of the tumors did not correlate with the risk grade. The only exception to this was the correlation between risk grade and the enhancement degree of the tumor after Gd‐DTPA. The ADC values were, respectively, (1.04 ± 0.13) × 10−3 mm2·s−1, (1.59 ± 0.06) × 10−3 mm2·s−1 and (1.94 ± 0.08) × 10−3 mm2·s−1 (P < 0.05) in the high‐, medium‐, and low‐risk grade groups. The ADC values of GISTs decreased with the increase of the risk grade of the tumors (r = −0.957; P < 0.05).Conclusion:DWI can be used to assess the risk grade of GISTs, but conventional MR imaging is of limited use. J. Magn. Reson. Imaging 2012; 36:1395–1401. © 2012 Wiley Periodicals, Inc.
Artifact reduction from metallic dental materials in T1‐weighted spin‐echo imaging at 3.0 tesla
Authors: Zho Sang‐Young , Kim Min‐Oh , Lee Keun‐Woo , Kim Dong‐Hyun 
Published: September 2012

AbstractPurpose:To investigate and propose a method of artifact reduction arising from metallic dental materials by applying a slice‐encoding for metal artifact correction (SEMAC) technique on T1‐weighted spin‐echo (SE) imaging at 3 Tesla.Materials and Methods:The view angle tilting (VAT) technique was adapted to conventional T1‐weighted spin‐echo (SE) sequence to correct the in‐plane distortion, and the SEMAC technique was used for correcting the remaining through‐plane distortions. Fourier transform based B0 field simulations were performed to estimate the amount of field perturbation and a scout imaging method was developed which guide in selecting the number of slice‐encodings needed in SEMAC sequences. Phantoms of six different dental materials with various shapes and sizes that are used in practice (amalgam; titanium implant; gold and Ni‐Cr crowns; Ni‐Ti and stainless steel orthodontic wires) were imaged. In vivo images of two subjects were also acquired. The amounts of artifact reduction were quantified in phantom studies.Results:Compared with conventional SE imaging in phantom studies, in‐plane artifacts were reduced by up to 43% in the VAT SE images and 80% in the SEMAC images. Through‐plane artifacts were reduced by up to 65% in SEMAC images. In vivo SEMAC images also showed reduced artifacts.Conclusion:The SEMAC technique can mitigate artifact caused by metallic dental materials for T1w‐SE imaging. J. Magn. Reson. Imaging 2013;37:471–478. © 2012 Wiley Periodicals, Inc.
Asymptomatic prospective and retrospective cohorts with metal‐on‐metal hip arthroplasty indicate acquired lymphocyte reactivity varies with metal ion levels on a group basis
Authors: Hallab Nadim J. , Caicedo Marco , McAllister Kyron , Skipor Anastasia , Amstutz Harlan , Jacobs Joshua J. 
Published: September 2012

AbstractSome tissues from metal‐on‐metal (MoM) hip arthroplasty revisions have shown evidence of adaptive‐immune reactivity (i.e., excessive peri‐implant lymphocyte infiltration/activation). We hypothesized that, prior to symptoms, some people with MoM hip arthroplasty will develop quantifiable metal‐induced lymphocyte reactivity responses related to peripheral metal ion levels. We tested three cohorts (Group 1: n = 21 prospective longitudinal MoM hip arthroplasty; Group 2: n = 17 retrospective MoM hip arthroplasty; and Group 3: n = 20 controls without implants). We compared implant position, metal‐ion release, and immuno‐reactivity. MoM cohorts had elevated (p < 0.01) amounts of serum Co and Cr compared to controls as early as 3 months post‐op (Group 1:1.2 ppb Co, 1.5 ppb Cr; Group 2: 3.4 ppb Co, 5.4 ppb Cr; Group 3: 0.01 ppb Co, 0.1 ppb Cr). However, only after 1–4 years post‐op did 56% of Group 1 develop metal‐reactivity (vs. 5% pre‐op, metal‐LTT, SI > 2), compared with 76% of Group 2, and 15% of Group 3 controls (patch testing was a poor diagnostic indicator with only 1/21 Group 1 positive). Higher cup‐abduction angles (50° vs. 40°) in Group 1 were associated with higher serum Cr (p < 0.07). However, sub‐optimal cup‐anteversion angles (9° vs. 20°) had higher serum Co (p < 0.08). Serum Cr and Co were significantly elevated in reactive versus non‐reactive Group‐1 participants (p < 0.04). CD4+CD69+ T‐helper lymphocytes (but not CD8+) and IL‐1β, IL‐12, and IL‐6 cytokines were all significantly elevated in metal‐reactive versus non‐reactive Group 1 participants. Our results showed that lymphocyte reactivity to metals can develop within the first 1–4 years after MoM arthroplasty in asymptomatic patients and lags increases in metal ion levels. This increased metal reactivity was more prevalent in those individuals with extreme cup angles and higher amounts of circulating metal. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 173–182, 2013
A consistent model for the thermal decomposition of NCN3 and the singlet– triplet relaxation of NCN
Authors: Dammeier J. , Oden B. , Friedrichs G. 
Published: September 2012

AbstractThe thermal decomposition of cyanogen azide (NCN3) and the subsequent collision‐induced intersystem crossing (CIISC) process of cyanonitrene (NCN) have been investigated by monitoring excited electronic state 1NCN and ground state 3NCN radicals. NCN was generated by the pyrolysis of NCN3 behind shock waves and by the photolysis of NCN3 at room temperature. Falloff rate constants of the thermal unimolecular decomposition of NCN3 in argon have been extracted from 1NCN concentration–time profiles in the temperature range 617 K <T< 927 K and at two different total densities: k(ρ ≈ 3 × 10−6 mol/cm3)/s−1=4.9 × 109 × exp (−71±14 kJ mol−1/RT) (± 30%); k(ρ ≈ 6 × 10−6 mol/cm3)/s−1=7.5 × 109 × exp (‐71±14 kJ mol−1/RT) (± 30%). In addition, high‐temperature 1NCN absorption cross sections have been determined in the temperature range 618 K <T< 1231 K and can be expressed by σ /(cm2/mol)= 1.0 × 108 −6.3 × 104 K−1 × T (± 50%). Rate constants for the CIISC process have been measured by monitoring 3NCN in the temperature range 701 K <T< 1256 K resulting in kCIISC (ρ ≈ 1.8 ×10−6 mol/cm3)/ s−1=2.6 × 106× exp (‐36±10 kJ mol−1/RT) (± 20%), kCIISC (ρ ≈ 3.5×10−6 mol/cm3)/ s−1 = 2.0 × 106 × exp (−31±10 kJ mol−1/RT) (± 20%), kCIISC (ρ ≈ 7.0×10−6 mol/cm3)/ s−1=1.4 × 106 × exp (−25±10 kJ mol−1/RT) (± 20%). These values are in good agreement with CIISC rate constants extracted from corresponding 1NCN measurements. The observed nonlinear pressure dependences reveal a pressure saturation effect of the CIISC process. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 45: 30–40, 2013
Evidence for the involvement of the anthranilate degradation pathway in Pseudomonas aeruginosa biofilm formation
Authors: Costaglioli Patricia , Barthe Christophe , Claverol Stephane , Brözel Volker S. , Perrot Michel , Crouzet Marc , Bonneu Marc , Garbay Bertrand , Vilain Sebastien 
Published: September 2012

AbstractBacterial biofilms are complex cell communities found attached to surfaces and surrounded by an extracellular matrix composed of exopolysaccharides, DNA, and proteins. We investigated the whole‐genome expression profile of Pseudomonas aeruginosa sessile cells (SCs) present in biofilms developed on a glass wool substratum. The transcriptome and proteome of SCs were compared with those of planktonic cell cultures. Principal component analysis revealed a biofilm‐specific gene expression profile. Our study highlighted the overexpression of genes controlling the anthranilate degradation pathway in the SCs grown on glass wool for 24 h. In this condition, the metabolic pathway that uses anthranilate for Pseudomonas quinolone signal production was not activated, which suggested that anthranilate was primarily being consumed for energy metabolism. Transposon mutants defective for anthranilate degradation were analyzed in a simple assay of biofilm formation. The phenotypic analyses confirmed that P. aeruginosa biofilm formation partially depended on the activity of the anthranilate degradation pathway. This work points to a new feature concerning anthranilate metabolism in P. aeruginosa SCs.
The Expected Impact of State Immigration Legislation on Labor Market Outcomes
Authors: Hotchkiss Julie L. , Quispe‐Agnoli Myriam 
Published: September 2012

AbstractIn response to the dramatic rise in the number of unauthorized immigrants to the United States, every state has passed some form of immigration legislation. These laws appear to be predicated on a belief that unauthorized immigrants impose greater costs than benefits to state and local communities, including the labor market. The purpose of this paper is to examine some evidence on what workers should expect if the immigration legislation is successful in eliminating undocumented workers from states' labor markets. © 2012 by the Association for Public Policy Analysis and Management.
Sensitivity of polyvinylidene fluoride films to mechanical vibration modes and impact after optimizing stretching conditions
Authors: Jain Anjana , Kumar Jayanth S. , Srikanth S. , Rathod V.T. , Roy Mahapatra D. 
Published: September 2012

AbstractThe β‐phase of polyvinylidene fluoride (PVDF) is well known for its piezoelectric properties. PVDF films have been developed using solvent cast method. The films thus produced are in α‐phase. The α‐phase is transformed to piezoelectric β‐phase when the film is hot‐stretched with various different stretching factors at various different temperatures. The films are then characterized in terms of their mechanical properties and surface morphological changes during the transformation from α‐ to β‐phases by using X‐ray diffraction, differential scanning calorimeter, Raman spectra, Infrared spectra, tensile testing, and scanning electron microscopy. The films showed increased crystallinity with stretching at temperature up to 80°C. The optimum conditions to achieve β‐phase have been discussed in detail. The fabricated PVDF sensors have been tested for free vibration and impact on plate structure, and its response is compared with conventional piezoelectric wafer type sensor. The resonant and antiresonant peaks in the frequency response of PVDF sensor match well with that of lead zirconate titanate wafer sensors. Effective piezoelectric properties and the variations in the frequency response spectra due to free vibration and impact loading conditions are reported. POLYM. ENG. SCI., 2013. © 2012 Society of Plastics Engineers
Characterisation of (E)‐2‐styrylchromones by electrospray ionisation mass spectrometry: singular gas‐phase formation of benzoxanthenones
Authors: Silva Eduarda M. P. , Domingues M. Rosário M. , Silva Artur M. S. , Cavaleiro José A. S. 
Published: September 2012

RATIONALEThe electrospray ionisation (ESI) mass spectra (ESI‐MSn, n = 2 and 3) of six (E)‐2‐styrylchromones containing electron‐donating and electron‐withdrawing groups were studied for the first time. The potential application of these compounds as drug candidates brings a need for the development of an analytical method to ensure a detailed knowledge of their structures.METHODSESI‐MS, CID‐MS2 and MS3 spectra of all the studied compounds were acquired using a LXQ linear ion trap mass spectrometer with an electrospray source, operating in the positive ion mode. ESI conditions were: nitrogen 8.00 sheath gas flow rate (arb), spray voltage 5 kV, heated capillary temperature 275 °C, capillary voltage 10.99 V and tube lens voltage 75.01 V. CID‐MS2 and MS3 experiments were performed on mass‐selected precursor ions using standard isolation and excitation procedures (activation q value of 0.25, activation time of 30 ms).RESULTSA comparable pattern of product ions was observed for all the studied compounds. Only the product ion (1,2)(3,4)A+ is common to all the studied compounds. Common to compounds 1 (R2 = H), 1a (R2 = OMe) and 1c (R2 = Cl) are the product ions formed by loss of H2O, CO, C6H5OH, and the product ions (1,2)(2,3)B+ and (1,2)(3,4)B+. Among all compounds, 2‐SC 1b (R2 = NO2) shows an obvious differentiation showing the lowest number of product ions under CID‐MS2 conditions.CONCLUSIONSThe analysed compounds revealed a comparable pattern of product ions formed for each compound only different in what concerns the relative abundance of these formed product ions. A rather interesting and unexpected loss of 2 Da was observed for some compounds. The structure of a benzoxanthenone was proposed for this product ion based on evidence obtained by synthesised benzoxanthenone as a model compound. Copyright © 2012 John Wiley & Sons, Ltd.