bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in the Biochemical Activity in Hairless Mouse Skin and Other Organs after Systemic and Topical Methotrexate Treatment
Authors: Yoshino Kazuhiro , Maibach Howard I. 
Published: April 2015

AbstractDNA synthesis in epidermis, spleen, small intestine, and muscle of the hairless mouse was measured after systemic and topical methotrexate dosing. Mice intraperitoneally injected with methotrexate (5 mg/Kg) incorporated 3H‐UdR in epidermis at 90% of baseline at 3 hrs, and the incorporation was not suppressed at 24 hrs. Muscle DNA synthesis was not suppressed. In the spleen and small intestine, incorporation was greatly suppressed to 10% at 3 hrs.On the other hand, after topically applied methotrexate (25 mg/Kg) treatment, epidermis and muscle were 60‐90% of baseline at 3‐24 hrs and spleen and small intestine were 5‐10% at 3‐6 hrs.In a prolonged time schedule study (4 days), epidermal incorporation after intraperitoneally applied methotrexate (5 mg/Kg) showed no suppression, but was instead stimulated to 180% at 2 days.5‐Fluorouracil, a thymidine kinase inhibitor in antitumor agents, was topically applied (25 mg/Kg) and compared for DNA synthesis. The incorporation of 3H‐UdR was drastically suppressed at 3 hrs.The results suggest that methotrexate is percutaneously absorbed, but does not extensively suppress epidermal DNA synthesis in hairless mice, although it does suppress spleen and intestinal DNA synthesis.
An Unusual Hybrid Cyst in Gardner's Syndrome with Partial Differentiation toward the Inner Root Sheath
Authors: Narisawa Yutaka , Kohda Hiromu 
Published: April 2015

AbstractA 26‐year‐old man with Gardner's syndrome had numerous subcutaneous nodules over almost his entire body. Histologically, one of them revealed an unusual so‐called hybrid cyst composed of epidermal type keratinization and inner root sheath type keratinization. The histologic findings and the possibility of cutaneous cysts based on hair matrix differentiation, more specifically partial differentiation toward the inner root sheath, are discussed.
Cutaneous Protothecosis
Authors: Otoyama Kazunobu , Tomizawa Nobuko , Higuchi Iwao , Horiuchi Yasuhiro 
Published: April 2015

AbstractThe present report describes a case of cutaneous protothecosis, as confirmed by skin biopsy and culture, in a 62‐year‐old man with an erythematous plaque lesion on his back. The disease was initially detected after return from a trip to Taiwan. The characteristic sporangia and biochemical sugar assimilation tests indicated the organism to be Prototheca wickerhamii. Oral administration of (5‐FC) failed to have any effect and so finally, the lesion had to be removed by surgical excision.
Multiple Seborrheic Verrucae Following Eczema
Authors: Horiuchi Yasuhiro 
Published: April 2015

AbstractOnly a limited number of cases of multiple seborrheic verrucae following eczema, or Williams' sign as first described by Williams in 1956, have been reported. The present report describes a 59‐year‐old man with multiple seborrheic verrucae following eczema.
A Case of Skin Metastasis of Lung Carcinoma Associated with Superior Vena Cava Syndrome
Authors: Matsukawa Ataru , Furusugi Yuzuru 
Published: April 2015

AbstractAn unusual case of lung carcinoma with both skin metastasis and superior vena cava syndrome (SVCS) is reported. The histological type of the primary lesion as taken by punch biopsy was small cell carcinoma consisting of relatively small cells with hyperchromatic nuclei and scanty cytoplasm. The cutaneous metastatic lesion consisted of large cells with light‐staining nuclei and small cells with deep‐staining nuclei; it had foci of glandular elements by light microscopy. Neurosecretory granules characteristic of small cell carcinoma were found in the tumor cell cytoplasm by electron microscopy. Cutaneous metastatic rates, complication rates of SVCS, and histological varieties of small cell lung carcinomas are discussed.
Immunoblot Demonstration of Protein Kinase C in Pig Epidermis
Authors: Koizumi Hiroko , Ohkawara Akira 
Published: April 2015

AbstractProtein kinase C, which has an important role in cell surface signal transduction, has at least 7 subspecies. The role of each isozyme remains unknown; however, the distribution of the subspecies has been reported to differ from tissue to tissue.In order to investigate the subspecies of protein kinase C in epidermis, we used monoclonal anti‐protein kinase C antibodies against the alpha and beta subspecies and against the gamma subspecies. Protein kinase C activity was detected in both cytosol and membrane bound fractions in the extract of pig epidermis, although with immunohistochemical analysis the enzyme was not detected in the epidermis. The protein kinase C in pig epidermis was extracted and partially purified with DE52 column chromatography and immunoblot analysis was performed. Western blot analysis with each anti‐protein kinase C antibody showed protein kinase C of the alpha and/or beta subspecies in pig epidermis. The gamma subspecies of the enzyme was not demonstrated. Since this gamma subspecies has only been demonstrated in the brain, it is regarded not to be present in epidermis.The differring patterns of the expression of the multiple subspecies of protein kinase C may closely related to its specific function in each tissue. The subspecies of the enzyme expressed in the epidermis should be clarified further.
Psoriatic Arthritis of the Hands in Seven Adults
Authors: Kato Naoko , Kato Sadatoshi , Tsukinaga Ichiro , Ando Masaaki , Ohkawara Akira 
Published: April 2015

AbstractPsoriatic arthritis is defined as an association of psoriasis with an arthropathy characterized mainly by osteolytic changes in the X‐ray. To assess its frequency among Japanese psoriatic patients and to characterize their signs and symptoms, we examined 51 patients (37 males, 14 females) for arthropathy of the fingers. Seven patients (5 males, 2 females) had abnormal X‐ray findings of the hands. They were compared with psoriatic patients without arthritis epidemiologically, clinically, and hematologically. Metacarpal indices were also measured and compared with the results from rheumatic arthritis patients and controls.
Sulfhydryl Drug‐induced Eruption: A Clinical and Histological Study
Authors: Kitamura Kazuko , Aihara Michiko , Osawa Junko , Naito Shizuo , Ikezawa Zenro 
Published: April 2015

AbstractSulfhydryl drug‐induced skin eruptions were studied clinically and histologically in 23 patients. In this study, tiopronin, D‐penicillamine, captopril and gold sodium thiomalate were considered to be sulfhydryl drugs, because they have a thiol group or release sulfhydryl compounds. The clinical features included skin eruptions that were maculopapular, erythema mutiforme‐like, eczematous, psoriasis‐like, seborrheic dermatitis‐like, Gibert‐like, lichen planus‐like, and pemphigus‐like. These clinical findings were reminiscent of the wide variety of eruptions seen in cutaneous graft‐versus‐host reactions (GVHR). Histologically, areas of vacuolation and eosinophilic necrosis with a satellite infiltrate of lymphoid cells were seen in the epidermis, and perivascular infiltrates were noted in the dermis. These findings were similar to the histological picture of cutaneous GVHR. In skin tests with sulfhydryl compounds, 19 out of 20 subjects showed positive reactions, and autoantibodies were found in 8 out of 12 subjects tested.Sulfhydryl drugs seem likely to induce immunologic changes in the host and to produce a distinctive reaction similar to that of cutaneous GVHR.
A Human Case of Zoonotic Onchocerciasis in Japan
Authors: Hashimoto Hiroyuki , Murakami Isamu , Fujiwara Sakuhei , Takayasu Susumu , Takaoka Hiroyuki , Uga Shoji , Akao Nobuaki , Kondo Kaoru , Yoshimura Hiroyuki 
Published: April 2015

AbstractA 2‐year‐old girl living in southwestern Japan had a nodule of 2 months' duration on the left foot. A biopsy from the lesion showed transverse sections of a worm surrounded by granulomatous tissue. The worm was identified as an Onchocerca sp. from the morphological characteristics such as relatively thick cuticles, annular ridges on the cortical layer, and high somatic muscles. Positive serological tests using ELISA for Onchocerca gutturosa and Onchocerca volvulus supported the diagnosis. This was the first case of zoonotic Onchocerca infection detected in Japan. The clinicopathological aspects of zoonotic onchocerciasis of this case were discussed.
A Peculiar Form of Epidermotropism in Cutaneous Metastatic Carcinoma
Authors: Oku Tomozo , Nakayama Fukiko , Takigawa Masahiro 
Published: April 2015

AbstractA 71‐year‐old man developed metastatic cutaneous nodules of colon carcinoma on the scalp and chest wall. Histopathological findings revealed aggressive invasion of the tumor cells into the overlying epidermis (epidermotropic carcinoma); they occasionally adhered to the epidermal cells. These morphological features have not been described in cutaneous metastases from internal carcinomas.
Amounts of Fibrous Proteins and Matrix Substances in Hairs of Different Races
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractFibrous proteins (FPs) and matrix substances (MSs) were extracted as S‐carboxymethylated (SCM) derivatives from scalp hair of five individuals each of the Mongoloid, Negroid and Caucasoid races. The ratio of the extracted SCM FPs to SCM MSs in dry weight was calculated for each hair specimen. The ratios obtained from the Mongoloid hair were 0.45 ± 0.03; those from the Negroid, 0.18 ± 0.02; and those from the Caucasoid, 0.29 ± 0.02. It has thus been suggested that the ratios of FP to MS contents in the hair may be involved in the determination of the macroscopic racial variations in the form of hair.
1,24(R)‐Dihydroxyvitamin D3, a Novel Active Form of Vitamin D3 with High Activity for Inducing Epidermal Differentiation but Decreased Hypercalcemic Activity
Authors: Matsunaga Takashi , Yamamoto Masaru , Mimura Hirohide , Ohta Tomohiro , Kiyoki Mamoru , Ohba Takeo , Naruchi Tatsuyuki , Hosoi Junichi , Kuroki Toshio 
Published: April 2015

Abstract1α,25‐Dihydroxyvitamin D3 (1,25(OH)2D3) is known to be a hormonally active form of vitamin D3 in the regulation of intracellular and extracellular calcium levels and of differentiation of myeloid cells and epidermal keratinocytes. We found that 1α,24(R)‐dihydroxyvitamin D3 (1,24(OH)2D3), a novel synthetic derivative of vitamin D3, is also active in regulating the differentiation of epidermal keratinocytes. 1,24(OH)2D3 had the same affinity as 1,25(OH)2D3 for a receptor isolated from the epidermis of newborn mice. The incubation of mouse epidermal keratinocytes with 1,24(OH)2D3 induced their differentiation in a time‐ and dose‐dependent manner, as determined by the formation of a cornified envelope and an increase in the activity of transglutaminase. 1,24(OH)2D3 inhibited DNA synthesis of epidermal keratinocytes and also increased their cytosolic calcium level. These effects of 1,24(OH)2D3 were similar to, or rather more than, those of physiologically active 1,25(OH)2D3. However, 1,24(OH)2D3 was found to cause less hypercalcemia than 1,25(OH)2D3 when administrated intravenously to rats, suggesting its possible therapeutic value in psoriasis.
Eosinophilic Polymyositis Induced by Tranilast
Authors: Arase Seiji , Kato Shoji , Nakanishi Hideki , Sadamoto Yasushi , Urano Yoshio , Kawai Hisaomi , Takeda Katsuyuki 
Published: April 2015

AbstractA 38‐year‐old man with asthma developed eosinophilic polymyositis following the administration of Tranilast, an antiasthmatic agent. Low grade fever, erythematous rashes on the entire body, dysphagia, blood eosinophilia, elevations of serum creatine phosphokinase and myoglobin levels, and inverted T waves in the electrocardiogram were noted. A muscle biopsy showed focal degeneration of muscle fibers with an infiltrate of eosinophils and lymphocytes. A rechallenge with Tranilast resulted in erythema formation, blood eosinophilia, and elevations of some serum muscle enzymes and myoglobin levels. Tranilast was considered to be the causative agent. This is the first reported case of Tranilast‐induced eosinophilic polymyositis.
Cutaneous T Cell Lymphoma Differing from Classical Mycosis Fungoides and Sézary Syndrome: Clinical, Histological and Immunohistochemical Studies of Six Cases
Authors: Maeda Kazuo , Takahashi Makoto , Takatsuka Noriko , Miyazawa Hitoshi 
Published: April 2015

AbstractSix cases of cutaneous T cell lymphoma (CTCL), differing from classical mycosis fungoides (MF) and Sézary syndrome (SS), were studied. Initial skin eruptions were erythematoplaques, papules and tumors. Some cases showed frequent spontaneous regressions without any specific therapy; however, the prognosis was not always good. Surface phenotypes of neoplastic cells were markedly heterogeneous from the onset of initial skin eruption. Three cases expressed the T helper/inducer (Th/i) phenotype, one case had both the Th/i and T suppressor/cytotoxic phenotypes, and the other two cases lacked both surface markers. These results suggest that CTCL other than MF and SS displays marked heterogeneous clinical behavior and diverse morphological and immunophenotypical characteristics of the postthymic T cells.
A New Apparatus for Hair Regrowth in Male‐pattern Baldness
Authors: Toshitani Shoji , Nakayama Juichiro , Yahata Takashi , Yasuda Masaru , Urabe Harukuni 
Published: April 2015

AbstractA newly devised apparatus (Scalp‐Tension‐Relaxer, STR) can efficiently promote hair regrowth in patients with male‐pattern baldness. When this apparatus is applied, the scalp is pushed up to relieve tension on the vertex. The efficacy rate of hair regrowth in alopecia patients was 40%. An investigation into the basis for the hair regrowth caused by this apparatus was directed toward the changes in hemodynamics and skin temperature of the scalp. During and after use of this apparatus, subjects exhibited an increase both in the cutaneous blood flow rate (as determined by laser Doppler flowmetry) and in the cutaneous temperature (as determined by thermography).
Immunopathological Aspects of Etretinate Therapy in Lichen Planus
Authors: Simon Miklós 
Published: April 2015

AbstractBiopsy specimens of involved and uninvolved skin were studied in 8 patients suffering from generalized lichen planus (LP) before and after successful etretinate treatment using murine monoclonal antibodies against several cell surface markers of effector and/or accessory cells of the immune system. In contrast to untreated LP‐lesions, in biopsies obtained after etretinate therapy from healed involved skin a markedly disintegrated HLA‐DR expression on keratinocytes and intraepidermal Langerhans cells and a nearly total loss of suppressor/cytotoxic T lymphocytes at the dermo‐epidermal interface were found. Our data emphasize the key position of the class II antigen expression on various immunocompetent cells, as a marker for a cell‐mediated immune reaction, on the other hand, stress the involvement of keratinocytes, Langerhans cells and suppressor/cytotoxic T lymphocytes in the pathogenesis of LP.
Immunohistochemical Study of Lysozyme in Various Benign Sweat Apparatus Tumors
Authors: Katsumata Michio , Ezoe Kazuhiko 
Published: April 2015

AbstractWe investigated the existence of lysozyme in various sweat apparatus tumors by adopting the avidin‐biotin‐peroxidase complex method. Positive reactions for lysozyme were found in four cases of apocrine cystadenoma, hidradenoma papilliferum, and an apocrine sweat apparatus benign tumor resembling “apocrine spiradenoma”, all of which derive from apocrine sweat apparatus. On the other hand, in ten cases of syringoma, eccrine hidrocystoma, clear cell hidradenoma, eccrine spiradenoma, and eccrine poroma, which derive from eccrine sweat apparatus, no positive stainings for lysozyme were obtained. In four out of five cases of mixed tumor of the skin, the apocrine type exhibited positive results. Two cases of syringocystadenoma papilliferum were negative for lysozyme. The investigation of lysozyme in various sweat apparatus tumors is useful in determining the direction of differentiation in these tumors.
Unusual Varicella Zoster Virus Infection in a Patient with Colon Carcinoma and Evans Syndrome
Authors: Hata Seiichiro , Tamaki Toshiharu 
Published: April 2015

AbstractA 53‐year‐old Japanese woman with Evans syndrome and colon cancer had two episodes of herpes zoster. The first painful vesicular rashes involved the right lower abdomen and buttock and healed in one month. After one more month, a second attack occurred on the right thigh and leg and developed into generalized hemorrhagic lesions, which became crusted in about 90 days. The patient died 131 days after the second attack, when the lesions had almost subsided. Varicella‐zoster virus (VZV) was isolated on the 59th day of the second attack. Her intracutaneous reactions to VZV antigen was negative, but the humoral antibodies were continuously positive.
A Case of Pyoderma Vegetans Associated with Acne Conglobata
Authors: Fujita Masaru , Okamoto Shoji 
Published: April 2015

AbstractA 41‐year‐old man visited our clinic because of vegetating plaques resembling pemphigus vegetans on his sacral and perianal areas. He had also suffered from acne conglobata since adolescence. A biopsy specimen taken from the perianal area showed marked acanthosis, exocytosis, and inflammatory infiltrates in the dermis, but no acantholysis. Direct and indirect immunofluorescent tests were negative for anti‐intercellular antibodies. Bacterial cultures from the lesions showed many different types of organisms. We diagnosed the case as pyoderma vegetans and discussed the relationship between pyoderma vegetans and other pyoderma.
Adenylate Cyclase System in Fetal Rat Keratinizing Epidermal Cells (FRSK Cells) and SV40‐transformed Human Keratinocytes
Authors: Takahashi Hidetoshi , Tamura Toshiya , Tsutsui Masato , Iizuka Hajime 
Published: April 2015

AbstractThe adenylate cyclase system of FRSK cells, a cultured cell line of fetal rat epidermal keratinocytes, and SV40‐transformed human keratinocytes was investigated. Stimulators of the human epidermal adenylate cyclase, epinephrine, adenosine, and prostaglandin E2 increased cyclic AMP levels of these cells. There were marked differences in the stimulatory effects; while epinephrine revealed a much stronger effect than the other stimulators in FRSK cells, epinephrine and prostaglandin E2 revealed similarly marked effects in SV40‐transformed cells. Histamine had little or only slight effect on the cyclic AMP levels of these cells. Cholera toxin and forskolin, which work on the stimulatory guanine nucleotide binding protein (Gs) and the catalytic component of adenylate cyclase, respectively, also increased cyclic AMP levels. Northern blot hybridization analysis revealed that both FRSK cells and SV40‐transformed human keratinocytes express mRNAs for the beta2‐adrenergic receptor, as well as the stimulatory and inhibitory guanine nucleotide binding proteins (Gs and Gi, respectively). The presence of Gs as well as Gi were confirmed by cholera toxin‐, and pertussis toxin (IAP)‐induced ADP‐ribosylation of membranous proteins of these cells. Our results indicate that both FRSK cells and SV40‐transformed human keratinocytes express the fundamental components of the adenylate cyclase system. These cell lines might be useful tools for the analysis of the adenylate cyclase system in epidermal keratinocytes.
Infiltration of Melanoma Cells into the Type I Collagen Gel
Authors: Kono Takeshi , Furukawa Masayoshi , Tanii Tsukasa , Taniguchi Shoji , Mizuno Nobuyuki , Ishii Masamitsu , Hamada Toshio 
Published: April 2015

AbstractMelanoma cells were cultured on type I collagen gel, and the infiltration of those cells into the gel was observed. B16 murine melanoma cells initially adopted a spherical form on the gel, but they assumed a dendritic form after infiltration into the interior. The degree of infiltration increased very rapidly and was time‐dependent. No correlations between the growth rate or melanogenic activity and infiltrative potential were observed. When Syrian hamster and human melanoma cell lines were cultured, the degrees of infiltration varied. This culture system using collagen gel is considered to be a useful in vitro model of tumor cell invasion.
Treatment of PUVA‐ and Retinoid‐PUVA‐resistant Severe Psoriasis with Systemic Cyclosporin A
Authors: Takashima Akira , Morita Akimichi , Tsuji Takuo 
Published: April 2015

AbstractTwenty patients with severe psoriasis were treated with the oral administration of 5 mg/kg/day of cyclosporin A (CyA) for 12 weeks. These patients had either failed to respond to or had become unresponsive to conventional treatments including PUVA, UVB, or combinations of etretinate and PUVA or UVB. Complete clearance and marked improvement were observed in 12 (60%) and 4 patients (20%), respectively. The average score of the Psoriasis Area and Severity Index (PASI) was 26.2 before treatment, decreasing to 18.3 in 2 weeks, 8.2 in 6 weeks, and 5.1 in 12 weeks of CyA treatment. There was a tendency for patients with lower blood levels of CyA to show smaller decreases in their PASI scores. In four patients who received skin biopsies, histological improvement was noted within 10 days of treatment; epidermal thickness had decreased by 32%, intraepidermal mitoses by 66%, and parakeratosis had disappeared almost completely. No clinical side effects or alterations in laboratory values were observed that required cessation of CyA. Exacerbations of psoriasis occurred in 11 of 16 patients within 6 weeks after stopping treatment. These results suggest that CyA could be the first choice of treatment for resistant severe psoriasis.
Acquired Perforating Dermatosis: Comparison of an Acquired Perforating Dermatosis and Perforation as an Incidental Histologic Finding
Authors: Kato Naoko 
Published: April 2015

AbstractA 66‐year‐old Japanese woman with a rare acquired perforating disorder, usually called adult‐type reactive perforating collagenosis, is reported. The patient had poorly controlled diabetes mellitus with retinopathy under oral diabetic medication. She was found to have multiple papules and umbilicated nodules on the trunk and four extremities when she was admitted and examined for the origin of jaundice and severe pruritus of sudden onset. In the biopsy specimen, collagen fibers were observed to be eliminated from the dermis through epidermal tunnel‐like perforations. No elastic fibers were eliminated, and serial sectioning of the specimen could not prove follicular perforation. Adenocarcinoma of the biliary duct was found to be the cause of the jaundice with pruritus. Although such cases are usually classified as acquired reactive perforating collagenosis of adult onset, proposed reclassification for acquired perforating disorders is discussed. Another case which also showed perforation and transepithelial elimination of both collagen and elastic fibers as an incidental histologic finding is described. Such elimination seems to be a not uncommon step in the formation of pruriginous eruptions. Therefore, these cases should be differentiated from acquired‐type characteristic perforating disorders.
A Combined Case of Desmoplastic Trichoepithelioma and Nevus Cell Nevus
Authors: Fukui Yoshimasa , Ono Hiroki , Umemura Tadahiro , Hasegawa Morimasa 
Published: April 2015

AbstractA 41‐year‐old woman with desmoplastic trichoepithelioma associated with pigmented nevus presented. Pigmented nevus had been present on her face at birth. She had received cryotherapy and dermabrasion at a small part of the pigmented nevus. As a result of the therapy, the discoloration disappeared. The lesion lately increased and became hard. Histologically, the lesion was composed of two distinctive but intimately mixed cellular components; nevus cells and basaloid cells. In the pigmented lesion, basaloid cells were not present. To our knowledge, this is the first reported case of desmoplastic trichoepithelioma associated with nevocellular nevus in Japan.