bluuebunny/crossref_metadata_2025_split huggingface dataset:
FOXP2-immunoreactive, corticothalamic pyramidal cells in neocortical layers 6a and 6b are tightly regulated by neuromodulatory systems
Authors: Qi Guanxiao , Yang Danqing , Messore Fernando , Bast Arco , Yáñez Felipe , Oberlaender Marcel , Feldmeyer Dirk 
Published: June 2024

SummaryTheFOXP2/Foxp2gene is involved in fine motor control in many vertebrate species; in humans, it is one of the candidate genes thought to play a prominent role in language production. Several studies suggest that in the neocortex,Foxp2is exclusively expressed in a subset of corticothalamic (CT) pyramidal cells (PCs) in layer 6 (L6). However, the morphological and intrinsic electrophysiological, synaptic and neuromodulatory properties ofFoxp2-expressing L6 PCs remain largely unknown. Here we systematically characterise these properties for FOXP2-positive (FOXP2+) PCs across L6 in the rat somatosensory cortex. We find that L6 FOXP2+ PCs are distinct in all of these properties from those of L6 FOXP2-negative (FOXP2–) neuronal cell types. We show that L6 FOXP2+ PCs project exclusively to thalamus. In upper L6 (L6a), FOXP2+ PCs innervate either the first-order thalamus or both first and higher-order thalamic nuclei. FOXP2+ PCs in deep L6 (L6b) project almost exclusively to higher-order thalamus. Synaptic connections established by L6a and L6b FOXP2+ PCs exhibit low synaptic release probability, whereas L6 corticocortical PCs have a high release probability. Both L6a and L6b FOXP2+ PCs respond strongly to acetylcholine (ACh), which in the absence of TTX results in firing of action potential (AP) trains. Notably, L6b but not L6a FOXP2– PCs are highly sensitive to ACh. In addition, L6b FOXP2+ PCs close to the white matter border show strong responses to dopamine that develop into prolonged AP firing. Our data suggest that FOXP2 is a marker for CT PCs in L6 that are strongly controlled by neurotransmitters such as ACh and dopamine. These findings are in line with a pivotal role for both L6a and L6b CT PCs as modulators of thalamic activity.
Visually Evoked 40 Hz Gamma Activity Enhanced by Transcranial Electrical Stimulation
Authors: Hainke Laura , Spitschan Manuel , Priller Josef , Taylor Paul , Dowsett James 
Published: June 2024

AbstractBackgroundNon-invasive Visual Stimulation (VS) and Transcranial Electrical Stimulation (TES) can modulate neuronal oscillations, including gamma activity at 40 Hz, which is relevant for cognition and disrupted in dementia. Combining both techniques may increase effects, but simultaneously recording Electroencephalography (EEG) activity poses several challenges, so this approach is untested.ObjectivesWe predicted that combined TES and VS in the lower gamma band would increase Steady-State Visually Evoked Potential (SSVEP) amplitude during and after stimulation, but only when targeting visual areas and at closely matching stimulation frequencies.MethodsWe administered combined VS and TES and simultaneously measured effects on EEG gamma activity in healthy participants. In experiment 1 (N=25), VS and TES frequencies were closely matched at ∼40 Hz, and TES sites varied between occipito-central, centro-occipital (reversed polarity), and centro-frontal. In experiment 2 (N=25), occipito-central TES was applied at ∼40 Hz, and VS frequency varied between 35, 40, and 45 Hz. Every 5-minute VS+TES trial was preceded and followed by a VS-only baseline trial. Electrical artifacts were removed using adaptive template subtraction.ResultsTES enhanced gamma SSVEP amplitudes most when applied to occipital and central sites, compared to frontal. Enhancement only occurred when TES and VS frequencies closely matched at ∼40 Hz, not when VS was slower (35 Hz) or faster (45 Hz) than TES. The effect was present during, not after, TES.ConclusionMultimodal visual and electrical stimulation evokes stronger oscillatory gamma activity than visual alone. Non-invasive gamma stimulation against cognitive decline in dementia may benefit from this optimised approach.
Previously uncharacterized aliphatic amino acid positions modulate the apparent catalytic activity of the EAL domain of ZMO_1055 and other cyclic di-GMP specific EAL phosphodiesterases
Authors: Cao Lianying , Yang Yong-Fu , Bai Feng-wu , Römling Ute 
Published: June 2024

AbstractThe ubiquitous second messenger cyclic di-GMP is the most abundant diffusible nucleotide signalling system in bacteria deciding the life style transition between sessility and motility. GGDEF diguanylate cyclases and EAL phosphodiesterases conventionally direct the turnover of this signaling molecule. Thereby, those domains are subject to micro- and macroevolution with the evolutionary forces that promote alterations in these proteins currently mostly unknown. While the highly conserved signature amino acids involved in divalent ion binding and catalysis equally as signal transduction modules have been readily identified, more subtle amino acid substitutions that modulate the catalytic activity have been rarely recognized and their molecular mechanism characterized. Our previous work identified the A526V substitution to be involved in downregulation of the apparent catalytic activity of theZymomonas mobilisZM4 PAS-GGDEF-EAL ZMO1055 phosphodiesterase and leading to a self-flocculation phenotype mediated by elevated production of the exopolysaccharide cellulose inZ. mobilisZM401. As A526 is located at a position that has previously not been recognized to affect the catalytic activity of the EAL domain, we further investigated the molecular mechanisms and the functional conservation of this substitution. Using a number of model systems, our results indicate that the alanine at position 526 is highly conserved in ZMO1055 homologs and beyond with the A526V mutation to alter the apparent phosphodiesterase activity in subgroups of EAL domains. Thus we hypothesize that single amino acid substitutions that lead to alterations in the catalytic activity of cyclic di-GMP turnover domains amplify the signaling output and thus significantly contribute to the flexibility and adaptability of the cyclic di-GMP signaling network. In this context, ZMO1055 seems to be a current evolutionary target.
Molecular basis of Ad5-nCoV Vaccine-Induced Immunogenicity
Authors: Dong Dongyang , Song Yutong , Wu Shipo , Wang Busen , Peng Cheng , Kong Weizheng , Zhang Zheyuan , Hou Li-Hua , Li Sai 
Published: June 2024

AbstractIn response to coronavirus disease 2019 (COVID-19), numerous vaccines have been developed to protect against SARS-CoV-2 infection. Ad5-nCoV (Convidecia) is a vaccine listed for emergency use by the WHO and has been administrated to millions of people globally. It comprises a series of human adenovirus 5 (Ad5) replication-incompetent vectored vaccines that transduce the spike protein (S) gene of various SARS-CoV-2 strains. Despite promising clinical data demonstrating its safety and effectiveness, the underlying molecular mechanism of its high immunogenicity and incidence of adverse reactions remains less understood. Here we combined cryo-ET, fluorescence microscopy and mass spectrometry to characterize thein situstructures, density and site-specific glycan compositions of the Ad5-nCoV_Wu and Ad5-nCoV_O vaccine-induced S antigens, which encode the unmodified SARS-CoV-2 Wuhan-Hu-1 S gene and optimized Omicron S gene, respectively. We found that the vaccine-induced S are structurally intact, antigenic and densely distributed on the cell membrane. Compared to Ad5-nCoV_Wu induced S, the Ad5-nCoV_O induced S demonstrate significantly better stability and is less likely to induce syncytia among inoculated cells. Our work demonstrated that Ad5-nCoV is a prominent platform for antigen induction and cryo-ET can be a useful technique for vaccine characterization and development.
CIEVaD: a lightweight workflow collection for rapid and on demand deployment of end-to-end testing of genomic variant detection
Authors: Krannich Thomas , Ternovoj Dimitri , Paraskevopoulou Sofia , Fuchs Stephan 
Published: June 2024

The identification of genomic variants has become a routine task in the thriving age of genome sequencing. Particularly small genomic variants of single or few nucleotides are routinely investigated for their impact on an organism’s phenotype. Hence, precise and robust detection of the variants’ exact genomic location and change in nucleotide composition is vital in many biological applications. Although a plethora of methods exist for the many key steps of variant detection, thoroughly testing the detection process and evaluating its results is still a cumbersome procedure. In this work, we present a collection of trivial to apply and highly modifiable workflows to facilitate the generation of synthetic test data as well as to evaluate the accordance of a user-provided set of variants with the test data.AvailabilityThe workflows are implemented in Nextflow and are freely available and open-source athttps://github.com/rki-mf1/cievadunder the GPL-3.0 license.
Colibactin-induced damage in bacteria is cell contact independent
Authors: Lowry Emily , Mitchell Amir 
Published: June 2024

AbstractThe bacterial toxin colibactin, produced primarily by the B2 phylogroup ofEscherichia coli,underlies some cases of colorectal cancers. Colibactin crosslinks DNA and induces genotoxic damage in both mammalian and bacterial cells. While the mechanisms facilitating colibactin delivery remain unclear, results from multiple studies supported a delivery model that necessitates cell-cell contact. We directly tested this requirement in bacterial cultures by monitoring the spatiotemporal dynamics of the DNA damage response using a fluorescent transcriptional reporter. We found that in mixed-cell populations, DNA damage saturated within twelve hours and was detectable even in reporter cells separated from colibactin producers by hundreds of microns. Experiments with distinctly separated producer and reporter colonies revealed that the intensity of DNA damage decays similarly with distance regardless of colony contact. Our work reveals that cell contact is inconsequential for colibactin delivery in bacteria and suggests that contact-dependence needs to be reexamined in mammalian cells as well.ImportanceColibactin is a bacteria-produced toxin that binds and damages DNA. It has been widely studied in mammalian cells due to its potential role in tumorigenesis. However, fundamental questions about its impact in bacteria remain underexplored. We usedE. colias a model system to study colibactin toxicity in neighboring bacteria and directly tested if cell-cell contact is required for toxicity, as has previously been proposed. We found that colibactin can induce DNA damage in bacteria hundreds of microns away and that the intensity of DNA damage presents similarly regardless of cell-cell contact. Our work further suggests that the requirement for cell-cell contact for colibactin-induced toxicity also needs to be reevaluated in mammalian cells.
ATP-dependent citrate lyase Drives Left Ventricular Dysfunction by Metabolic Remodeling of the Heart
Authors: Liu Shijie , Gammon Seth T. , Tan Lin , Gao Yaqi , Kim Kyoungmin , Williamson Ian K. , Pham Janet , Davidian Angela , Khanna Radhika , Gould Benjamin D. , Salazar Rebecca , Vitrac Heidi , Dinh An , Lien Evan C. , de L. Vitorino Francisca N. , Gongora Joanna M. , Martinez Sara A. , Lawrence Czer S. C. , Kransdorf Evan P. , Leffer David , Hanson Blake , Garcia Benjamin A. , Vander Heiden Matthew G. , Lorenzi Philip L. , Taegtmeyer Heinrich , Piwnica-Worms David , Martin James F. , Karlstaedt Anja 
Published: June 2024

ABSTRACTBackgroundMetabolic remodeling is a hallmark of the failing heart. Oncometabolic stress during cancer increases the activity and abundance of the ATP-dependent citrate lyase (ACL,Acly), which promotes histone acetylation and cardiac adaptation. ACL is critical for the de novo synthesis of lipids, but how these metabolic alterations contribute to cardiac structural and functional changes remains unclear.MethodsWe utilized human heart tissue samples from healthy donor hearts and patients with hypertrophic cardiomyopathy. Further, we used CRISPR/Cas9 gene editing to inactivateAclyin cardiomyocytes of MyH6-Cas9 mice.In vivo,positron emission tomography andex vivostable isotope tracer labeling were used to quantify metabolic flux changes in response to the loss of ACL. We conducted a multi-omics analysis using RNA-sequencing and mass spectrometry-based metabolomics and proteomics. Experimental data were integrated into computational modeling using the metabolic network CardioNet to identify significantly dysregulated metabolic processes at a systems level.ResultsHere, we show that in mice, ACL drives metabolic adaptation in the heart to sustain contractile function, histone acetylation, and lipid modulation. Notably, we show that loss of ACL increases glucose oxidation while maintaining fatty acid oxidation.Ex vivoisotope tracing experiments revealed a reduced efflux of glucose-derived citrate from the mitochondria into the cytosol, confirming that citrate is required for reductive metabolism in the heart. We demonstrate that YAP inactivation facilitates ACL deficiency. Computational flux analysis and integrative multi-omics analysis indicate that loss of ACL induces alternative isocitrate dehydrogenase 1 flux to compensate.ConclusionsThis study mechanistically delineates how cardiac metabolism compensates for suppressed citrate metabolism in response to ACL loss and uncovers metabolic vulnerabilities in the heart.
Dead reckoning: charting a new (metaphorical) course for accounting
Authors: Hutchinson Robert , Amador Carlos 
Published: June 2024

Purpose
Metaphor is the foundation upon which all scientific disciplines, from basic to applied, construct the mental models used in theory development and organizing research phenomena. The authors posit that a navigational science metaphor might provide a useful framework, or at least an additional “waypoint,” with which to evaluate extant accounting theory and further discourse in accounting research and practice. This study aims to critically examine the base metaphors of accounting theory and practice through the lens of navigational science.

Design/methodology/approach
The supreme dominance of the Positive Accounting Theory paradigm (Watts and Zimmerman, 1986) is critically evaluated using a navigational metaphor as a literary device for cognitive estrangement.

Findings
The authors suggest that accounting, as both a practical and academic field, might benefit from the multifarious approach of navigational science in the computation of longitude, particularly with regards to the use of external (societal) referents, moving toward a more “heteroglossic” model of accounting (vid. Macintosh and Baker, 2002) as a means of “situating” accounting research and practice with regards to said external referents (cf. Bayou et al., 2011).

Originality/value
This work brings together existing streams of literary theory and epistemology in accounting, and views them through the lens of a navigational science metaphor. Cognitive estrangement, a well-established device for reorganizing perplexing problems in any science, is used to reimagine an accounting science as navigational situating.
Electronic procurement adoption and procurement performance: does institutional quality matter?
Authors: Charnor Isaac Tetteh , Quartey Evelyn Kukuwa 
Published: June 2024

PurposeElectronic procurement has received primacy as one of the significant reforms to help fight corruption and inefficiencies in the public sector across countries. The purpose of this paper is to examine the effect of electronic procurement adoption on procurement performance. Additionally, the paper examines the moderating role of institutional quality in the relationship between electronic procurement and procurement performance.Design/methodology/approachThe study applied an explanatory design and a quantitative research approach. Also, the study relied on UTAUT and institutional theory to develop a test research model using 121 responses from public entities in Ghana. Hypothesised paths were investigated using structural equation modelling.FindingsThe findings revealed that electronic procurement adoption positively and significantly affects procurement performance. Also, the findings indicated that institutional quality positively and significantly affects electronic procurement adoption and procurement performance, respectively. Lastly, the findings proved that institutional quality moderates the relationship between electronic procurement adoption and procurement performance.Research limitations/implicationsThe study focused on public procurement entities in the Greater Accra Region – Ghana. The study contributes to adopting electronic procurement, institutional quality, and procurement performance literature. The study also extends the UTAUT and the institutional theory to African public entities. The study reechoes the importance of institutional quality in developing economies to enhance electronic procurement adoption.Originality/valueTo the best of the researcher’s knowledge, this is the first study that examines the moderating role of institutional quality in the relationship between electronic procurement adoption and procurement performance.
Literature review: Industry 5.0. Leveraging technologies for environmental, social and governance advancement in corporate settings
Authors: Rehman Ali , Umar Tariq 
Published: June 2024

Purpose
This research paper explores the transformative potential of Industry 5.0 for environmental, social and governance (ESG) factors within corporate settings. This study aims to elucidate the role of Industry 5.0 and its related technologies in influencing ESG factors, explore potential risks linked to ESG and present strategies for mitigation through Industry 5.0.

Design/methodology/approach
This paper is the literature review that introduces Industry 5.0 as a pivotal factor in implementing and mitigating ESG and its related risks. It outlines Industry 5.0's characteristics, driven by advanced technologies.

Findings
Literature reviews suggest that Industry 5.0 has the potential to significantly influence ESG factors within corporate settings. It can promote sustainability, enhance working conditions and offer operational advantages.

Practical implications
The practical implications of this research paper are twofold. First, it provides valuable insights to policymakers, organizations and regulatory bodies, guiding them in adapting their frameworks to embrace Industry 5.0. This adaptation is essential for achieving ESG goals and facilitating sustainable development. Second, it highlights the critical role of Industry 5.0 in mitigating ESG-related risks, offering a robust structure for sustainable development.

Originality/value
This research paper contributes to the existing body of knowledge by highlighting the transformative potential of Industry 5.0 in the context of ESG. It offers a comprehensive exploration of the historical evolution of corporate governance, the integration of sustainability and the growing focus on ESG. It also highlights the originality and value of Industry 5.0 as a critical mitigating factor for ESG-related risks, presenting a holistic approach to sustainable corporate practices.
A new stress tensor approach for application to the conductor surface
Authors: Petkovć Bojana , Ziolkowski Marek , Toepfer Hannes , Haueisen Jens 
Published: June 2024

Purpose
The purpose of this paper is to derive a new stress tensor for calculating the Lorentz force acting on an arbitrarily shaped nonmagnetic conductive specimen moving in the field of a permanent magnet. The stress tensor allows for a transition from a volume to a surface integral for force calculation.

Design/methodology/approach
This paper derives a new stress tensor which consists of two parts: the first part corresponds to the scaled Poynting vector and the second part corresponds to the velocity term. This paper converts the triple integral over the volume of the conductor to a double integral over its surface, where the subintegral functions are continuous through the different compartments of the model. Numerical results and comparison to the standard volume discretization using the finite element method are given.

Findings
This paper evaluated the performance of the new stress tensor computation on a thick and thin cuboid, a thin disk, a sphere and a thin cuboid containing a surface defect. The integrals are valid for any geometry of the specimen and the position and orientation of the magnet. The normalized root mean square errors are below 0.26% with respect to a reference finite element solution applying volume integration.

Originality/value
Tensor elements are continuous throughout the model, allowing integration directly over the conductor surface.
Empowering women through digital technology: unraveling the nexus between digital enablers, entrepreneurial orientation and innovations
Authors: Abdelwahed Nadia A. Abdelmegeed , Bano Safia , Al Doghan Mohammed A. , Aljughiman Abdulaziz Ahmed , Shah Naimatullah , Soomro Bahadur Ali 
Published: June 2024

PurposeWomen's empowerment plays a pivotal role in achieving sustainable and sustainable development in developed and developing contexts. The present paper explores the effect of technology orientation (TO), entrepreneurial orientation (EO), and digital technology self-efficacy (DTSE) on digital innovation (DI) and women's empowerment (WE) among Saudi women.Design/methodology/approachThis is a cross-sectional study which applies a deductive approach. The study collected data from women in Saudi Arabia actively involved in entrepreneurship and utilizing digital technology. The survey questionnaire is used as a prevalent tool to get responses. Finally, the study concludes based on 316 valid samples.FindingsThe structural equation modeling through SmartPls4, the results exert an insignificant effect of TO on both DI and women empowerment. The study confirmed a positive significant impact of EO on DI but not on WE. Moreover, the DTSE construct is found to be a significant and robust analyst of DI and WE. With regard to mediating effects, DI mediates the relationship between EO, DTSE and WE, but not between TO and WE.Practical implicationsThe study's findings contribute to more comprehensive and effective initiatives that foster innovation, gender equality, and WE in entrepreneurial networks. The study would assist policymakers and planners in developing robust strategies focusing on digitalization to boost DI and WE through enhanced DTSE. The study would also offer guidelines for policymakers to achieve sustainable development goals (SDGs) generally and specifically for Saudi Vision 2030, which is particularly ambitious to promote WE.Originality/valueThe study fills the gaps by offering a bunch of predictors, i.e., TO, EO, DTSE and DI, which predict WE in the Saudi context.
Identification of regional growth poles: the case of Vietnam
Authors: Le Minh Son 
Published: June 2024

PurposeThis study aims to identify the location of regional growth poles in Vietnam.Design/methodology/approachA potential gravity model is constructed to estimate how attractive a location is in relation to other locations within a specifically defined region using spatial interpolation tools.FindingsWe present the calculated and visualized potential gravitational energy (or attractiveness) for every province showcasing regional growth poles in Vietnam.Research limitations/implicationsGraphical evidence need to be supported by statistical analysis to establish causal effects of driving factors on growth measures.Originality/valueThis is the first study to use a potential gravity model to study growth poles in Vietnam.