bluuebunny/crossref_metadata_2025_split huggingface dataset:
Congenital unilateral pericardial agenesis presenting as an isolated chest pain in an adolescent: a case report and comprehensive review
Authors: Jafari Farshad , Taheri Maryam , Ebrahimi Pouya , Soflaee Maedeh , Rafie Reyhaneh Alipore , Anafje Mohsen 
Published: February 2025

Abstract

Introduction
Congenital pericardial agenesis is a rare congenital anomaly resulting from the incomplete development of the pleuropericardial membranes during embryogenesis, leading to the partial or complete absence of the pericardial sac. Although this condition usually remains asymptomatic, it can present with various misleading symptoms such as chest pain (add some other, maybe 2, more prevalent presenting symptoms), making diagnosis challenging. Advanced imaging techniques are crucial for accurate diagnosis and management, especially when usual diagnostic modalities do not achieve a definite diagnosis.

Case presentation
The history and diagnostic process of a 16-year-old female who presented with isolated, non-exertional chest pain are detailed. A comprehensive diagnostic work-up was initiated, including chest X-ray (CXR), transthoracic echocardiogram (TTE), CT angiography (CTA), and cardiac magnetic resonance imaging (CMRI). These advanced imaging modalities unveiled the rare and elusive diagnosis of left-sided pericardial agenesis, decisively ruling out other potential causes and shedding light on an extraordinary case that challenges conventional diagnostic pathways.

Conclusion
Unilateral pericardial agenesis, though typically benign and often shrouded in clinical obscurity, can manifest with enigmatic symptoms such as isolated chest pain, necessitating a meticulous and comprehensive diagnostic approach. Multimodal imaging is essential for accurate diagnosis and for ruling out complications such as cardiac herniation or coronary artery compression. Considering the absence of significant complications, conservative management was chosen in this case, with the patient being discharged with instructions to monitor for any warning signs.

Clinical key message
Clinicians should consider congenital pericardial agenesis as one of the potential causes of unexplained chest pain, particularly when the initial investigations are inconclusive. Advanced imaging techniques (such as CXR and MRI) are vital for confirming the diagnosis and subsequently appropriate and timely management and preventing potential complications.
Refined variant calling pipeline on RNA-seq data of breast cancer cell lines without matched-normal samples
Authors: Eberth Sonja , Koblitz Julia , Steenpaß Laura , Pommerenke Claudia 
Published: February 2025

Abstract

Objective
RNA-seq delivers valuable insights both to transcriptional patterns and mutational landscapes for transcribed genes. However, as tumour cell lines frequently lack their matched-normal counterpart, variant calling without the paired normal sample is still challenging. In order to exclude variants of common genetic variation without a matched-normal control, filtering strategies need to be developed to identify tumour relevant variants in cell lines.

Results
Here, variants of 29 breast cancer cell lines were called on RNA-seq data via HaplotypeCaller. Low read depth sites, RNA-edit sites, and low complexity regions in coding regions were excluded. Common variants were filtered using 1000 genomes, gnomAD, and dbSNP data. Starting from hundred thousands of single nucleotide variants and small insertions and deletions, about thousand variants remained after filtering for each sample. Extracted variants were validated against the Catalogue of Somatic Mutations in Cancer (COSMIC) for 10 cell lines included in both data sets. Approximately half of the COSMIC variants were successfully called. Importantly, missing variants could mainly be attributed to sites with low read depth. Moreover, filtered variants also included all 10 cancer gene census COSMIC variants, a condensed hallmark variant set.
ROASMI: accelerating small molecule identification by repurposing retention data
Authors: Sun Fang-Yuan , Yin Ying-Hao , Liu Hui-Jun , Shen Lu-Na , Kang Xiu-Lin , Xin Gui-Zhong , Liu Li-Fang , Zheng Jia-Yi 
Published: February 2025

Abstract
The limited replicability of retention data hinders its application in untargeted metabolomics for small molecule identification. While retention order models hold promise in addressing this issue, their predictive reliability is limited by uncertain generalizability. Here, we present the ROASMI model, which enables reliable prediction of retention order within a well-defined application domain by coupling data-driven molecular representation and mechanistic insights. The generalizability of ROASMI is proven by 71 independent reversed-phase liquid chromatography (RPLC) datasets. The application of ROASMI to four real-world datasets demonstrates its advantages in distinguishing coexisting isomers with similar fragmentation patterns and in annotating detection peaks without informative spectra. ROASMI is flexible enough to be retrained with user-defined reference sets and is compatible with other MS/MS scorers, making further improvements in small-molecule identification.
IHCH9033, a novel class I HDAC inhibitor, synergizes with FLT3 inhibitor and rescues quizartinib resistance in FLT3-ITD AML via enhancing DNA damage response
Authors: Yao Mingyue , Yan Wenzhong , Wang Yafang , Zhao Yu , Xu Xiaowei , Chen Yujun , Yu Chengcheng , Li Yingnian , Jiang Hualiang , Shen Jie , Cheng Jianjun , Xie Chengying 
Published: February 2025

Abstract

Background
Despite initial success with FLT3 inhibitors (FLT3is), outcomes for FLT3-ITD acute myeloid leukemia (AML) patients remain unsatisfactory, underscoring the need for more effective treatment options. Epigenetic modifications, such as histone acetylation, contribute to AML’s onset and persistence, advocating the potential for epigenetic therapies. However, the poor specificity of pan-histone deacetylase inhibitors (HDACis) leads to undesirable adverse effects, prompting the need for isoform-specific HDACis. This study aims to explore the antileukemic activities and mechanisms of IHCH9033, a novel class I HDACi, alone or combined with FLT3i in FLT3-ITD AML.

Methods
The viability of AML cell lines and primary AML cells treated with HDACis alone or in combination with FLT3i was detected by MTT or CCK8 assay. Flow cytometry was utilized to examine cell apoptosis, cell cycle progression and ROS production. RNA sequencing analysis, RT-qPCR, western blotting, and co-immunoprecipitation assays were employed to elucidate the molecule mechanisms. The in vivo anti-leukemia efficacy was tested in xenografted mice models derived from FLT3-ITD cell lines and primary AML patients.

Results
Here, we identified IHCH9033, a novel selective class I HDACi, which exhibited an increased antitumor effect in FLT3-ITD AML through effectively eliminating leukemia burden and overcoming resistance to FLT3i. Mechanically, IHCH9033 selectively inhibited DNA repair in FLT3-ITD AML cells, leading to the accumulation of DNA damage that eventually resulted in cell cycle arrest and apoptosis. Additionally, IHCH9033 induced HSP90 acetylation, FLT3 ubiquitination, and proteasomal degradation of FLT3, thereby inhibiting FLT3 downstream signaling. Notably, IHCH9033 maintained its potency in both FLT3i-resistant AML cell lines and primary-resistant patient samples, and exerted strong synergy with the FLT3i quizartinib, leading to tumor regression in FLT3-ITD/TKD AML xenografts. In patient-derived xenografts, the treatment with IHCH9033, both alone and in combination, led to nearly complete eradication of the AML burden, without significant adverse effects.

Conclusions
Our study shows that IHCH9033, a novel class I HDACi with a desirable pharmacological profile, is a promising drug candidate for FLT3-ITD AML, and suggests a strategy of combining class I HDACis and FLT3is in AML clinical trials to increase efficacy and overcome resistance, thus potentially providing a curative treatment option.
Recent advances in CMOS-compatible synthesis and integration of 2D materials
Authors: Katiyar Ajit Kumar , Choi Jonggyu , Ahn Jong-Hyun 
Published: February 2025

Abstract
The upcoming generation of functional electronics in the era of artificial intelligence, and IoT requires extensive data storage and processing, necessitating further device miniaturization. Conventional Si CMOS technology is struggling to enhance integration density beyond a certain limit to uphold Moore’s law, primarily due to performance degradation at smaller dimensions caused by various physical effects, including surface scattering, quantum tunneling, and other short-channel effects. The two-dimensional materials have emerged as highly promising alternatives, which exhibit excellent electrical and mechanical properties at atomically thin thicknesses and show exceptional potential for future CMOS technology. This review article presents the chronological progress made in the development of two-dimensional materials-based CMOS devices with comprehensively discussing the advancements made in material production, device development, associated challenges, and the strategies to address these issues. The future prospects for the use of two-dimensional materials in functional CMOS circuitry are outlooked, highlighting key opportunities and challenges toward industrial adaptation.

Graphical Abstract
Antibacterial and physicomechanical properties of cellulosic nonwovens functionalized with chitosan: a study on interaction effects of influencing factors and assessment methods
Authors: Gebeyehu Esubalew Kasaw , Shresth Rekha , Saha Tonmoy , Tienaho Jenni , Jauhiainen Ulla , Tarhini Ali Amin , Tehrani-Bagha Ali Reza 
Published: February 2025

Abstract
The growing demand for antimicrobial textiles and environmental concerns over synthetic agents have driven interest in biobased agents like chitosan, an eco-friendly alternative reported with variable effectiveness on textiles. This research investigates the effects of chitosan’s molecular weight, concentration, treatment method, and their interaction effects on the antibacterial and physicomechanical properties of cellulosic nonwovens. The role of culture medium dynamics in antibacterial testing was also examined. Chitosan with low (30 kDa), medium (250 kDa) and high (2100 kDa) molecular weights at concentrations of 1 to 15 g/L was applied using pad-dry and dip-dry methods, with dip coating resulting in higher chitosan deposition. Antibacterial activity against Escherichia coli (E. coli) was assessed using three methods: agar diffusion, a luminescent bacterial biosensor assay, and log reduction of colony-forming units (CFU/mL) in a time-kill test. While the first two test methods showed no antibacterial effect, CFU/mL revealed significant activity with interaction effects showing that the dip-coated, 30 kDa chitosan above a concentration of 5 g/L achieved complete bacterial reduction. The enhanced antibacterial performance of chitosan-coated cellulosic substrates, compared to chitosan in a culture medium, highlighted the role of cellulosic nonwoven in boosting antibacterial effectiveness, likely through improved contact and interaction with bacterial cells. The study demonstrated that chitosan-treated cellulosic nonwovens, particularly with dip-dry coating altered tensile strength and increased the bending resistance and bending stiffness.

Graphical Abstract
Harnessing the synergy of statistics and deep learning for BCI competition 4 dataset 4: a novel approach
Authors: Jangir Gauttam , Joshi Nisheeth , Purohit Gaurav 
Published: February 2025

Abstract
Human brain signal processing and finger’s movement coordination is a complex mechanism. In this mechanism finger’s movement is mostly performed for every day’s task. It is well known that to capture such movement EEG or ECoG signals are used. In this order to find the patterns from these signals is important. The BCI competition 4 dataset 4 is one such standard dataset of ECoG signals for individual finger movement provided by University of Washington, USA. In this work, this dataset is, statistically analyzed to understand the nature of data and outliers in it. Effectiveness of pre-processing algorithm is then visualized. The cleaned dataset has dual polarity and gaussian distribution nature which makes Tanh activation function suitable for the neural network BC4D4 model. BC4D4 uses Convolutional neural network for feature extraction, dense neural network for pattern identification and incorporating dropout & regularization making the proposed model more resilient. Our model outperforms the state of the art work on the dataset 4 achieving 0.85 correlation value that is 1.85X (Winner of BCI competition 4, 2012) & 1.25X (Finger Flex model, 2022).
Mice Lacking the Fructose Transporter Glut5 Exhibit Excessive Androgens and Reduced Sperm Motility
Authors: Kallianioti Aikaterini , Bourdon Guillaume , Grandhaye Jeremy , Chevaleyre Claire , Aboulouard Soulaimane , Péchoux Christine , Ribes Sandy , Sellem Eli , Ramé Christelle , Plotton Ingrid , Fournier Isabelle , Salzet Michel , Dupont Joelle , Douard Véronique , Froment Pascal 
Published: February 2025

Abstract
Overconsumption of fructose is linked to metabolic diseases, which are often associated with reduced fertility. GLUT5 is the most specific fructose transporter. To investigate its role in the testes, we analyzed the male reproductive phenotype of transgenic male mice deficient in GLUT5 (GLUT5−/− or GLUT5 knockout [KO] mice). Glut5 expression was shown in Leydig cells and germ cells, from primary spermatocytes to spermatozoa. We found reduced intratesticular fructose and pyruvate concentrations in GLUT5−/− mice. These mice exhibited 30% lower litter sizes compared with control mice. Histological analysis of the testes revealed some seminiferous tubules with a “Sertoli cell-only” phenotype, although spermatogenesis occurred normally in most tubules. Reduced fertility in GLUT5 KO mice was linked to lower sperm production and impaired sperm quality. Spermatozoa from these mice displayed reduced motility, head abnormalities, and a diminished acrosome reaction, which was associated with reduced cyclic adenosine monophosphate content and impaired phosphorylation of protein kinase A substrates in the acrosome. Unexpectedly, androgen production in GLUT5 KO mice was 3-fold higher than in controls, despite unchanged luteinizing hormone levels. Electron microscopy of Leydig cells revealed a highly developed smooth endoplasmic reticulum, increased lipid droplets, and abnormal mitochondrial structures, suggesting disrupted mitochondrial dynamics. Proteomic analysis identified 155 deregulated proteins in the testicular tissue of GLUT5 KO mice, nearly half of which were associated with sperm motility, germ cell morphology, glycolysis, mitochondrial dynamics, and oxidative stress. In conclusion, the absence of the specific fructose transporter GLUT5 reduced testicular fructose content and led to an asthenozoospermia phenotype accompanied by hyperandrogenism.
The Interaction and Mediation of Physical Activity of Body Mass Index with Cardiovascular Disease: evidence from NHANES and MR Analysis
Authors: Zhao Liang , Zhang Danfeng , Zhang Ting , Wang Chunhun , Han Shuo , Zhang Tengfei , He Zhiqing , Wang Junyu 
Published: February 2025

ABSTRACT

Background
Both insufficient physical activity (PA) and excess body weight are important risk factors for cardiovascular disease (CVD), and PA is closely related to body weight. However, it remains unclear whether PA modifies or mediates the association of body mass index (BMI) with CVD.

Methods
We conducted a cross-sectional study of 35,406 adults participating in the National Health and Nutrition Examination Survey (NHANES). The mediation and interaction effects of PA were assessed using a four-way decomposition approach. An additional two-step Mendelian randomization (MR) analysis was performed to verify the potential causal mediation effect.

Results
A strong association was observed between PA and lower odds of CVD after adjusting for all confounders [Odds Ratio (OR): 0.84; 95% Confidence Interval (CI): 0.74–0.95]. Increased BMI was associated with higher odds of CVD (OR: 1.04; 95% CI: 1.03–1.04). PA showed interaction and mediation effects on the association of BMI with CVD. The overall proportion attributable to interaction was -37.5%, while the overall proportion attributable to mediation was 22.2%. MR analysis further confirmed that PA causally mediated the pathway from BMI to CVD.

Conclusions
PA modified the association of BMI with CVD, suggesting that sufficient PA is needed to lower the impact of high BMI on CVD risk. Moreover, we found that PA served as a causal influence on the association of BMI with CVD, indicating that higher BMI led to a lower level of PA, which in turn increased the risk of CVD.
Are health professionals ‘equipped’ to support vegan mothers in the UK?
Authors: Francis Bethany , Miele Mara 
Published: February 2025

This article investigates the extent to which health professionals are equipped to support vegan mothers in the UK and how stigma plays a part in this issue. Veganism has grown in popularity in the UK in recent years (as well as in many other countries worldwide) with the environmental, health and animal welfare benefits of the lifestyle becoming more widely researched and publicised. However, research into the topic of veganism has highlighted that, up until recently, many vegans experienced stigma for their beliefs, with the UK being no exception. Similarly, a growing body of research has highlighted the long-accepted and gendered issue of mum-shaming, whereby mothers, as opposed to fathers, are disproportionately criticised and blamed for their parenting skills. In this article we want to examine the experience of women who fit into both categories of veganism and motherhood in their encounters with health professionals during pregnancy and while raising their babies on a vegan diet.
Adopting semi-structured interviews, this study gauged the opinions of a sample of vegan mothers, non-vegan mothers, midwives and paediatricians. Our findings indicate that in the UK, healthcare professionals are not equipped to provide vegan mothers with nutritional advice. This was found to be due to a lack of understanding of veganism, which, in some cases, fuelled negative ideas on veganism and led to stigma towards vegan pregnant women and vegan mothers. All healthcare professionals interviewed stressed the severe lack of training on vegan nutrition, as well as nutrition more generally.
Predicting Free Flap Viability: Integrating Lactate and Glucose Measurements with Artificial Intelligence
Authors: Muzakkie Mufida , Murti Krisna , Nurmaini Siti , Soedjana Hardi Siswo 
Published: February 2025

Introduction: In reconstructive surgery, free flaps are a superior method for resurfacing defects. While free flap viability is typically monitored by subjective clinical examination, lactate and glucose levels in free flaps, which can affect tissue metabolism during ischaemia and reperfusion, can help predict viability. This study aims to review previous research and provide a theoretical basis for using artificial intelligence in lactate and glucose measurement as a means of assessing flap viability.Method: The primary databases used to retrieve the key medical literature presented in this study were book references and Google Scholar, PubMed and Science Direct, using search terms related to the topic. Only articles written in English and published less than ten years ago were included.Results: Lactate levels detect perfusion impairment earlier than clinical signs or other biochemical markers while glucose monitoring can indicate underlying metabolic dysregulation or physiological stress, helps early detection of complications. Combining lactate and glucose measurements enhances diagnostic accuracy and allows for timely interventions for flap viability. Studies confirm this dual monitoring is a practical, unbiased, and has the potential to be developed into an artificial intelligence tool to improve patient outcomes.Conclusion: Lactate and glucose measurements in free flap monitoring have distinct benefits. Lactate detects ischaemia and reflects tissue metabolism, while glucose monitors energy metabolism and systemic health. Combining these leading to improved flap survival rates. With accessible tools, this approach improves patient care and outcomes in reconstructive surgery.
Rapid Resolution of Perianal Crohn's Disease with Upadacitinib
Authors: Brennan Kristen , Beniwal-Patel Poonam 
Published: February 2025

ABSTRACT
A 20-year-old woman with ileocolonic, stricturing, and penetrating Crohn's disease (CD) presented with new onset perianal and vulvar CD despite having a diverting ileostomy and medical therapy with ustekinumab. She had previous secondary nonresponse to infliximab, vedolizumab, and adalimumab. There is a paucity of data supporting the use of medical therapies for perianal CD. Upadacitinib is approved for treatment of moderate to severely active ulcerative colitis and CD. This case demonstrates successful and rapid resolution of perianal CD within weeks of initiating upadacitinib.
Evaluating The Visibility of Building Syrian Refugee Shelters by 3D Printing Technology in Jordan
Authors: Akeila Mohanad , Kuok King Kuok Kelvin , H. Matthew Wong N. 
Published: February 2025

Syrian Refugees in Jordan are placed all over the country. Low income refugees are placed in Jordanian camps that miss the basic living standards such as water, electricity, and stable sheltering system. The weakness of refugee camps come in association to the short execution period of construction. New technologies such as 3D printing are emerging with potential to provide better sheltering solutions; however, these technologies are not yet properly explored for providing sustainable shelters to Syrian refugees in Jordan and therefore, this paper developed to evaluate the efficiency of 3D printing technology in comparison to transitional shelters that is commonly used over Jordanian camp, through developing a 3D printable shelter model that assist in studying the cost, the technical specification, and delivery time of shelter printed by the technology. After the comparison, it was found that the 3D printed shelter achieved lower construction cost, higher durability, and can be executed within the same period of transitional shelters.Â Â
A Galerkin Approach to the Generalized Karush–Kuhn–Tucker Conditions for the Solution of an Elliptic Distributed Optimal Control Problem with Pointwise State and Control Constraints
Authors: Brenner Susanne C. , Sung Li-Yeng 
Published: February 2025

Abstract
We develop a convergence analysis for the simplest finite element method for a model linear-quadratic elliptic distributed optimal control problem with pointwise control and state constraints under minimal assumptions on the constraint functions.
We then derive the generalized Karush–Kuhn–Tucker conditions for the solution of the optimal control problem from the convergence results of the finite element method and the Karush–Kuhn–Tucker conditions for the solutions of the discrete problems.