bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tissue-Preserving Antibody Cocktails to Differentiate Primary Squamous Cell Carcinoma, Adenocarcinoma, and Small Cell Carcinoma of Lung
Authors: Brown Alan F. , Sirohi Deepika , Fukuoka Junya , Cagle Philip T. , Policarpio-Nicolas Maria , Tacha David , Jagirdar Jaishree 
Published: January 2013

Context.—With the availability of cell type–specific therapies, differentiating primary lung squamous cell carcinomas (SCCs) and adenocarcinomas (ACAs) has become important. The limitations of small sample size and the need to conserve tissue for additional molecular studies necessitate the use of sensitive and specific marker panels on a single slide.Objective.—To distinguish SCC from ACA and small cell carcinoma (SmCC) of lung using 2 novel tissue-conserving cocktails.Design.—We compared two antibody cocktails, desmoglein 3 + cytokeratin 5/napsin A and p40/thyroid transcription factor 1 (Biocare Medical, Concord, California) in diagnosing SCC and ACA of the lung on tissue microarray, cytology, and surgical specimens. Both lung and nonlung tissue were evaluated on an 1150-core tissue microarray that contained 200 lung cancers. A microarray of 35 SmCCs and 5 small cell SCCs was also evaluated.Results.—A cocktail of desmoglein 3 + cytokeratin 5/napsin A provided diagnostic accuracy in lung cancers with a sensitivity and specificity of 100% in SCCs and a sensitivity of 86% and a specificity of 100% in ACAs. A p40/thyroid transcription factor 1 cocktail showed p40 to have a specificity of 92% and a sensitivity of 93% in SCCs, whereas thyroid transcription factor 1 had a specificity of 100% and a sensitivity of 77% in ACAs. Cell blocks of fine-needle aspiration cytology compared with corresponding surgical (n = 20) specimens displayed similar findings. The p40 was useful in differentiating bladder from prostate carcinoma with 88% sensitivity. Isolated carcinomas from nonlung tissues were desmoglein 3 + cytokeratin 5 positive. Napsin A was positive in 22% of renal tumors as previously observed. Both cocktails were excellent in differentiating SmCCs and small cell SCCs because none of the SmCCs stained with p40.Conclusions.—Both antibody cocktails are excellent in differentiating primary lung ACA from SCC, as well as excluding SmCC and ACAs from all other sites on small specimens. A cocktail of desmoglein 3 + cytokeratin 5/napsin A is slightly superior compared with p40/thyroid transcription factor 1 cocktail.
Māori Medical Student and Physician Exposure to Racism, Discrimination, Harassment, and Bullying
Authors: Cormack Donna , Gooder Claire , Jones Rhiannon , Lacey Cameron , Stanley James , Paine Sarah-Jane , Curtis Elana , Harris Ricci 
Published: July 2024

ImportanceDiscrimination, bullying, and harassment in medicine have been reported internationally, but exposures for Indigenous medical students and physicians, and for racism specifically, remain less examined.ObjectiveTo examine the prevalence of racism, discrimination, bullying, and harassment for Māori medical students and physicians in New Zealand and associations with demographic and clinical characteristics.Design, Setting, and ParticipantsThis cross-sectional study used data from an anonymous national survey of Māori medical students and physicians in New Zealand in late 2021 and early 2022. Data were analyzed from March 2022 to April 2024.ExposuresAge, gender, marginalized status (ie, in addition to being Māori, belonging to other groups traditionally marginalized or underrepresented in medicine), year of medical school, year of graduation, and main work role.Main Outcomes and MeasuresDirect and witnessed racism, discrimination, bullying, and harassment were measured as any experience in the last year and ever. Any exposure to negative comments about social groups and witnessing discriminatory treatment toward Māori patients or whānau (extended family). Considering leaving medicine, including because of mistreatment, was measured.ResultsOverall, 205 Māori medical students (median [IQR] age, 23.1 [21.6-24.3] years; 137 [67.2%] women) and 200 physicians (median [IQR] age, 36.6 [30.1-45.3] years; 123 [62.8%] women) responded. Direct and witnessed exposure to racism (184 students [91.5%]; 176 physicians [90.7%]) and discrimination (176 students [85.9%]; 179 physicians [89.5%]) ever in medical education, training, or work environments was common. Ever exposure to witnessed and direct bullying (123 students [66.5%]; 150 physicians [89.3%]) and harassment (73 students [39.5%]; 112 physicians [66.7%]) was also common. Most respondents reported witnessing Māori patients or their whānau being treated badly in clinical settings, in direct interactions (67 students [57.8%]; 112 physicians [58.9%]) or behind their backs (87 students [75.0%]; 138 physicians [72.6%]). One-quarter of Māori medical students (45 students), and 37.0% of physicians (61 physicians) had considered leaving or taken a break from medicine because of these experiences. Additional marginalized statuses were significantly associated with any direct experience of mistreatment in the last year for students and physicians. Exposure to some forms of mistreatment were also significantly associated with higher likelihood of thinking about leaving or taking a break from medicine for physicians.Conclusions and RelevanceIn this study, Māori medical students and physicians reported high exposure to multiple forms of racism, discrimination, bullying, and harassment in medical education, training, and work environments, requiring an urgent response from medical institutions.
Phantoms of a Five‐day Forest
Authors: Badenoch Kirsty 
Published: July 2024

AbstractStrangely, the palimpsest of human occupation is often far less visible in the city than it is in wilder spaces. Artist, architect, researcher and educator Kirsty Badenoch describes an exploratory expedition to draw out these ghosts within the rugged landscapes of the Scottish Highlands, and a subsequent exhibition of the work through diary entries and photographic forms. The work itself is made through transcriptive collaborations with the volatile climate and flora and fauna of the site, creating a tale of palimpsestuous relationships that dislocate the artistic self as nature leads the dance, or at the very least calls the tune.
Immunomodulating Sonocatalytic Nanoagents with Dual‐Functional Ir‐N Centers and Narrow Bandgap for Reversing Immunosuppression and Potentiating Ovarian Cancer Immunotherapy
Authors: Huang Xiaotong , Zhou Hongju , Lv Ning , Zhao Zhenyang , Tian Tian , Huang Jiayan , Rodriguez Raul D. , Luo Hong , Cheng Chong , Ma Lang 
Published: July 2024

AbstractThe heterogeneity and immunosuppression microenvironment of ovarian cancer seriously restrict the efficiency of monomodal treatment. Emerging multimodal therapy strategies based on sonodynamic therapy with enhanced antitumor effects have attracted intensive attention in the quest to combat cancer. However, exploring highly efficient sonosensitizers integrating chemodynamic/sonodynamic/immunotherapies with reversing immunosuppressive tumor microenvironment (TME) capacity is urgently desired but remains challenging. Here, a facile strategy is designed to synthesize immunomodulating sonocatalytic nanoagents (IrT‐SCN) with dual‐functional Ir‐N centers and narrow bandgap for reversing immunosuppression and potentiating ovarian cancer immunotherapy. IrT‐SCN with dual‐functional centers Ir‐N2 and Ir‐N4 and conjugated networks show a reduced bandgap, moderate interaction with H2O2, and efficient production of reactive oxygen species (ROS). Such ROS capabilities include: 1) utilizing H2O2 in TME to catalytically generate potent O2, as well as abundant superoxide anion (•O2−) and singlet oxygen (1O2) radicals; 2) external ultrasound (US) irradiation can also boost the production of 1O2 simultaneously; 3) M1 polarization of tumor‐associated macrophages and enhanced immune effect can promote the outcome of cancer treatment. This finding provides proof‐of‐concept evidence for the future development of immunomodulating sonocatalytic nanoagents for oncological treatments and other ROS‐related biomedical fields.
Scaled Elastic Hydrogel Interfaces for Brain Electrophysiology
Authors: Hu Mengdi , Ren Jing , Pan Yue , Cheng Liping , Xu Xin , Tan Chee Leong , Sun Huabin , Shi Yi , Yan Shancheng 
Published: July 2024

AbstractThe advancement in brain–computer interface technology has facilitated real‐time communication between the brain and external devices, rapidly expanding invasive brain–computer interfaces. Nevertheless, little progress has been made in improving the one‐way, noninvasive brain–computer interface technology using conductive methods, necessitating enhancements to the current paste contact electrodes. In this paper, a captivating spherical electrode is fabricated that exhibits noncytotoxic properties, combining alginate hydrogel with indium tin oxide conductor components. The electrode's remarkable and consistent electrical conductivity enables it to generate stable adaptive deformations that conform to diverse scalp topographies. Most impedance values fall below 10 kΩ, indicating heightened hydrophilicity compared to traditional conductive pastes, as evidenced by contact angle measurements. An empirical electroencephalography data collection study confirms its conductivity and signal transmission reliability. The acquired electroencephalography signals are utilized for emotion recognition utilizing band energy ratio analysis and support vector machine (SVM) algorithms. The operation of this system is both straightforward and convenient, requiring minimal preparation before and after the collection of electroencephalography signals. Utilizing an extensive range of raw materials and straightforward preparatory procedures enhances the potential industrial application of hydrogel electrodes, thereby significantly contributing to advancing civilian electroencephalography equipment and investigating depression‐related pathologies.
Single Versus Blended Electrolyte Additives: Impact of a Sulfur‐Based Electrolyte Additive on Electrode Cross‐Talk and Electrochemical Performance of LiNiO2||Graphite Cells
Authors: Wölke Christian , Benayad Anass , Lai Thanh‐Loan , Hanke Felix , Baraldi Giorgio , Echeverría María , Esen Ekin , Ayerbe Elixabete , Neale Alex R. , Everitt Jacqui , Hardwick Laurence J. , Yan Peng , Poterała Marcin , Wieczorek Władysław , Winter Martin , Cekic‐Laskovic Isidora 
Published: July 2024

AbstractLithium nickel oxide (LNO) is an attractive positive electrode active material for lithium ion batteries (LIBs) due to its high reversible specific capacity and absence of cobalt. Nevertheless, it is prone to structural instabilities that lead to rapid capacity fading, safety concerns and shows in average a lower voltage than mixtures with cobalt, limiting its applicability to date. Herein this study introduces the sulfur‐based electrolyte additive, benzo[d][1,3,2]dioxathiole 2,2‐dioxide (DTDPh), to stabilize the LNO electrode and study its effects on interphase compositions by means of complementary electrochemical and spectroscopic techniques. Obtained results demonstrate an improved galvanostatic cycling performance in terms of cycle life and achievable specific discharge capacity that significantly outperform the common film‐forming additive vinylene carbonate (VC). The cycle life is increased from 102 to 147 cycles compared to the baseline electrolyte and the accumulated discharge energy until end of life is increased by 45%. This study furthermore provides strong evidence of a significant cross‐talk and negative interplay between DTDPh and VC when both are present in the electrolyte formulation. Mechanistic consideration based on density functional theory (DFT) calculations suggest the formation of mobile poly(VC) species, which is supported by the results of post mortem analysis of the resulting interphases.
Development of an animal model of hypothyroxinemia during pregnancy in Wistar rats
Authors: Wei Wei , Liu Aihua , Liu Min , Li Mingfeng , Wu Xinghan , Qin Chuan , Shan Zhongyan , Zhang Ling 
Published: July 2024

AbstractBackgroundHypothyroxinemia is a subclinical thyroid hormone deficiency in which the mother has inadequate levels of T4 during pregnancy. The fetus relies entirely on the mother's T4 hormone level for early neurodevelopment. Isolated maternal hypothyroxinemia (IMH) in the first trimester of pregnancy can lead to lower intelligence, lower motor scores, and a higher risk of mental illness in descendants. Here, we focus on the autism‐like behavior of IMH offspring.MethodsThe animals were administered 1 ppm of propylthiouracil (PTU) for 9 weeks. Then, the concentrations of T3, T4, and thyroid‐stimulating hormone (TSH) were detected using enzyme‐linked immunosorbent assay (ELISA) to verify the developed animal model of IMH. We performed four behavioral experiments, including the marble burying test, open‐field test, three‐chamber sociability test, and Morris water maze, to explore the autistic‐like behavior of 40‐day‐old offspring rats.ResultsThe ELISA test showed that the serum T3 and TSH concentrations in the model group were normal compared with the negative control group, whereas the T4 concentration decreased. In the behavioral experiments, the number of hidden marbles in the offspring of IMH increased significantly, the frequency of entering the central compartment decreased, and the social ratio decreased significantly.ConclusionThe animal model of IMH was developed by the administration of 1 ppm of PTU for 9 weeks, and there were autistic‐like behavior changes such as anxiety, weakened social ability, and repeated stereotyping in the IMH offspring by 40 days.
Lesions Causing Alice in Wonderland Syndrome Map to a Common Brain Network Linking Body and Size Perception
Authors: Friedrich Maximilian U. , Baughan Elijah C. , Kletenik Isaiah , Younger Ellen , Zhao Charlie W. , Howard Calvin , Ferguson Michael A. , Schaper Frederic L.W.V.J. , Chen Amalie , Zeller Daniel , Piervincenzi Claudia , Tommasin Silvia , Pantano Patrizia , Blanke Olaf , Prasad Sashank , Nielsen Jared A. , Fox Michael D. 
Published: July 2024

ObjectiveAlice in Wonderland syndrome (AIWS) profoundly affects human perception of size and scale, particularly regarding one's own body and the environment. Its neuroanatomical basis has remained elusive, partly because brain lesions causing AIWS can occur in different brain regions. Here, we aimed to determine if brain lesions causing AIWS map to a distributed brain network.MethodsA retrospective case–control study analyzing 37 cases of lesion‐induced AIWS identified through systematic literature review was conducted. Using resting‐state functional connectome data from 1,000 healthy individuals, the whole‐brain connections of each lesion were estimated and contrasted with those from a control dataset comprising 1,073 lesions associated with 25 other neuropsychiatric syndromes. Additionally, connectivity findings from lesion‐induced AIWS cases were compared with functional neuroimaging results from 5 non‐lesional AIWS cases.ResultsAIWS‐associated lesions were located in various brain regions with minimal overlap (≤33%). However, the majority of lesions (≥85%) demonstrated shared connectivity to the right extrastriate body area, known to be selectively activated by viewing body part images, and the inferior parietal cortex, involved in size and scale judgements. This pattern was uniquely characteristic of AIWS when compared with other neuropsychiatric disorders (family‐wise error‐corrected p < 0.05) and consistent with functional neuroimaging observations in AIWS due to nonlesional causes (median correlation r = 0.56, interquartile range 0.24).InterpretationAIWS‐related perceptual distortions map to one common brain network, encompassing regions critical for body representation and size‐scale processing. These findings lend insight into the neuroanatomical localization of higher‐order perceptual functions, and may inform future therapeutic strategies for perceptual disorders. ANN NEUROL 2024;96:662–674
Highly Selective Photocatalytic Synthesis of Acetic Acid at 0–25 °C
Authors: Zong Xupeng , Chu Yi‐Chun , Tang Yu , Li Yuting , Wu Xin‐Ping , Sun Zaicheng , Tao Franklin 
Published: July 2024

AbstractAcetic acid (AA), a vital compound in chemical production and materials manufacturing, is conventionally synthesized by starting with coal or methane through multiple steps including high‐temperature transformations. Here we present a new synthesis of AA from ethane through photocatalytic selective oxidation of ethane by H2O2 at 0–25 °C. The catalyst designed for this process comprises g‐C3N4 with anchored Pd1 single‐atom sites. In situ studies and computational simulation suggest the immobilized Pd1 atom becomes positively charged under photocatalytic condition. Under photoirradiation, the holes on the Pd1 single‐atom of OH−Pd1
/g‐C3N4 serves as a catalytic site for activating a C−H instead of C−C of C2H6 with a low activation barrier of 0.14 eV, through a concerted mechanism. Remarkably, the selectivity for synthesizing AA reaches 98.7 %, achieved under atmospheric pressure of ethane at 0 °C. By integrating photocatalysis with thermal catalysis, we introduce a highly selective, environmentally friendly, energy‐efficient synthetic route for AA, starting from ethane, presenting a promising alternative for AA synthesis. This integration of photocatalysis in low‐temperature oxidation demonstrates a new route of selective oxidation of light alkanes.
A Cost‐Effective Sulfide Solid Electrolyte Li7P3S7.5O3.5 with Low Density and Excellent Anode Compatibility
Authors: Li Hui , Lin Qiaosong , Wang Jinzhu , Hu Lv , Chen Fang , Zhang Zhihua , Ma Cheng 
Published: July 2024

AbstractThe commercialization of all‐solid‐state Li batteries (ASSLBs) demands solid electrolytes with strong cost‐competitiveness, low density (for enabling satisfactory energy densities), and decent anode compatibility (the need for cathode compatibility can be circumvented by the cathode coating techniques that are widely applied in sulfide‐based ASSLBs). However, none of the reported oxide, sulfide, or chloride solid electrolytes meets these requirements simultaneously. Here, we design a Li7P3S7.5O3.5 (LPSO) solid electrolyte, which shows a combination of all the aforementioned characteristics. The synthesis of this material does not need the expensive Li2S, so the raw materials cost is only $14.42/kg, which, unlike most solid electrolytes, lies below the $50/kg threshold for commercialization. The density of LPSO is 1.70 g cm−3, considerably lower than those of the oxide (typically above 5 g cm−3) and chloride (around 2.5 g cm−3) solid electrolytes. Besides, LPSO also shows excellent anode compatibility. The Li|LPSO|Li cell cycles stably with a potential of ~50 mV under 0.1 mA cm−2 for over 4200 h at 25 °C, and the all‐solid‐state pouch cell with the Si anode shows a capacity retention of 89.29 % after 200 cycles under 88.6 mA g−1 at 60 °C.
Unlocking the Power of Human Ferritin: Enhanced Drug Delivery of Aurothiomalate in A2780 Ovarian Cancer Cells
Authors: Cosottini Lucrezia , Geri Andrea , Ghini Veronica , Mannelli Michele , Zineddu Stefano , Di Paco Giorgio , Giachetti Andrea , Massai Lara , Severi Mirko , Gamberi Tania , Rosato Antonio , Turano Paola , Messori Luigi 
Published: July 2024

AbstractAurothiomalate (AuTM) is an FDA‐approved antiarthritic gold drug with unique anticancer properties. To enhance its anticancer activity, we prepared a bioconjugate with human apoferritin (HuHf) by attaching some AuTM moieties to surface protein residues. The reaction of apoferritin with excess AuTM yielded a single adduct, that was characterized by ESI MS and ICP‐OES analysis, using three mutant ferritins and trypsinization experiments. The adduct contains ~3 gold atoms per ferritin subunit, arranged in a small cluster bound to Cys90 and Cys102. MD simulations provided a plausible structural model for the cluster. The adduct was evaluated for its pharmacological properties and was found to be significantly more cytotoxic than free AuTM against A2780 cancer cells mainly due to higher gold uptake. NMR‐metabolomics showed that AuTM bound to HuHf and free AuTM induced qualitatively similar changes in treated cancer cells, indicating that the effects on cell metabolism are approximately the same, in agreement with independent biochemical experiments. In conclusion, we have demonstrated here that a molecularly precise bioconjugate formed between AuTM and HuHf exhibits anticancer properties far superior to the free drug, while retaining its key mechanistic features. Evidence is provided that human ferritin can serve as an excellent carrier for this metallodrug.
Tris(triazolo)triazine‐Based Covalent Organic Frameworks for Efficiently Photocatalytic Hydrogen Peroxide Production
Authors: Zhang Zhenwei , Zhang Qi , Hou Yuxin , Li Jiali , Zhu Shanshan , Xia Hong , Yue Huijuan , Liu Xiaoming 
Published: July 2024

AbstractTwo‐dimensional covalent organic frameworks (2D‐COFs) have recently emerged as fascinating scaffolds for solar‐to‐chemical energy conversion because of their customizable structures and functionalities. Herein, two tris(triazolo)triazine‐based COF materials (namely COF‐JLU51 and COF‐JLU52) featuring large surface area, high crystallinity, excellent stability and photoelectric properties were designed and constructed for the first time. Remarkably, COF‐JLU51 gave an outstanding H2O2 production rate of over 4200 μmol g−1 h−1 with excellent reusability in pure water and O2 under one standard sun light, that higher than its isomorphic COF‐JLU52 and most of the reported metal‐free materials, owing to its superior generation, separation and transport of photogenerated carriers. Experimental and theoretical researches prove that the photocatalytic process undergoes a combination of indirect 2e− O2 reduction reaction (ORR) and 4e− H2O oxidation reaction (WOR). Specifically, an ultrahigh yield of 7624.7 μmol g−1 h−1 with apparent quantum yield of 18.2 % for COF‐JLU52 was achieved in a 1 : 1 ratio of benzyl alcohol and water system. This finding contributes novel, nitrogen‐rich and high‐quality tris(triazolo)triazine‐based COF materials, and also designate their bright future in photocatalytic solar transformations.
Stereodivergent Total Synthesis of Tacaman Alkaloids
Authors: Chen Xiangtao , Wang Huijing , Zeng Jie , Li Qiuhong , Zhang Tonghui , Yang Qiaoyun , Tang Pei , Chen Fen‐Er 
Published: July 2024

AbstractThis paper describes a concise, asymmetric and stereodivergent total synthesis of tacaman alkaloids. A key step in this synthesis is the biocatalytic Baeyer–Villiger oxidation of cyclohexanone, which was developed to produce seven‐membered lactones and establish the required stereochemistry at the C14 position (92 % yield, 99 % ee, 500 mg scale). Cis‐ and trans‐tetracyclic indoloquinolizidine scaffolds were rapidly synthesized through an acid‐triggered, tunable acyl‐Pictet–Spengler type cyclization cascade, serving as the pivotal reaction for building the alkaloid skeleton. Computational results revealed that hydrogen bonding was crucial in stabilizing intermediates and inducing different addition reactions during the acyl‐Pictet–Spengler cyclization cascade. By strategically using these two reactions and the late‐stage diversification of the functionalized indoloquinolizidine core, the asymmetric total syntheses of eight tacaman alkaloids were achieved. This study may potentially advance research related to the medicinal chemistry of tacaman alkaloids.