bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of adjunctive roxithromycin therapy on interleukin‐1β, transforming growth factor‐β1 and vascular endothelial growth factor in gingival crevicular fluid of cyclosporine A‐treated patients with gingival overgrowth
Authors: Gong Y. , Lu J. , Ding X. , Yu Y. 
Published: August 2013

Background and ObjectiveSystemic macrolide antibiotic administration has been shown to result in the elimination or reduction cyclosporine A‐induced gingival overgrowth. Roxithromycin (ROX) is known to have anti‐inflammatory, immunomodulatory and tissue reparative effects. This study was to evaluate the effect of adjunctive ROX therapy on cyclosporine A‐induced gingival overgrowth and interleukin (IL)‐1β, transforming growth factor (TGF)‐β1 and vascular endothelial growth factor (VEGF) levels in gingival crevicular fluid of renal transplant patients.Material and MethodsThirty‐one patients with clinically significant overgrowth and 16 periodontally healthy subjects were included in this randomized, double‐blind, placebo‐controlled, parallel‐arm study. Patients received scaling and root planing (SRP) at baseline and randomized to take either ROX or placebo for 5 d. The clinical parameters, including plaque index, papillary bleeding index, probing depth and gingival overgrowth scores, were recorded. The amounts of IL‐1β, TGF‐β1 and VEGF in gingival crevicular fluid were detected by ELISA. Periodontal parameters as well as gingival crevicular fluid biomarker levels were evaluated at baseline and at 1 and 4 wk post‐therapy.ResultsFollowing SRP plus ROX and SRP plus placebo therapy, significant improvements in clinical periodontal parameters of both study groups were observed (p < 0.025). In the ROX group, adjunctive ROX therapy resulted in a greater gingival overgrowth scores reduction compared with those in the placebo group at 4 wk (p < 0.017). Initial amounts of IL‐1β, TGF‐β1 and VEGF for both the ROX and placebo groups were significantly higher than those for healthy subjects (p < 0.017), with no statistical difference between the two study groups. At 1 and 4 wk post‐therapy, significant decreases in the amounts of IL‐1β, TGF‐β1 and VEGF were observed in both study groups when compared with baseline (p < 0.025), but there was no difference in the levels of IL‐1β and VEGF between the two study groups. The amount of decrease in TGF‐β1 levels for the ROX group was statistically significant compared to that for the placebo group at 4 wk after treatment (p < 0.017).ConclusionOur study indicated that combination of ROX with non‐surgical therapy improves gingival overgrowth status and decreases gingival crevicular fluid TGF‐β1 levels in patients with severe gingival overgrowth. The reduction of gingival crevicular fluid TGF‐β1 following ROX therapy suggests an anti‐inflammatory/immunomodulatory effect of ROX on the treatment of cyclosporine A‐induced gingival overgrowth.
Chinese family management of chronic childhood conditions: A cluster analysis
Authors: Zhang Ying , Wei Min , Zhang Yaqing , Shen Nanping 
Published: August 2013

AbstractPurposeThis study compared the management of chronic childhood conditions in Chinese families and identified predictors of management style.Design and MethodA survey was conducted in 2008–2009 on a convenience sample of 387 caregivers of children with chronic conditions.Results. Six family management styles (FMSs) were identified: accomplished, uncertain managing, joint managing, managing with difficulty, struggling, and competent managing. The FMSs were related to the functioning of the family and the child. Changes in parents' employment, geographic location, and diagnosis predicted family placement in a cluster.Practice ImplicationsUnderstanding Chinese FMSs will further aid in providing culturally appropriate family‐centered care. Awareness of the predictors of FMSs can assist nurses in detecting which families need more help.
Body temperature, activity and melatonin profiles in adults with attention‐deficit/hyperactivity disorder and delayed sleep: a case–control study
Authors: Bijlenga Denise , Van Someren Eus J. W. , Gruber Reut , Bron Tannetje I. , Kruithof I. Femke , Spanbroek Elise C. A. , Kooij J. J. Sandra 
Published: August 2013

SummaryIrregular sleep–wake patterns and delayed sleep times are common in adults with attention‐deficit/hyperactivity disorder, but mechanisms underlying these problems are unknown. The present case–control study examined whether circadian abnormalities underlie these sleep problems in a naturalistic home setting. We included 12 medication‐naïve patients with attention‐deficit/hyperactivity disorder and delayed sleep phase syndrome, and 12 matched healthy controls. We examined associations between sleep/wake rhythm in attention‐deficit/hyperactivity disorder and circadian parameters (i.e. salivary melatonin concentrations, core and skin temperatures, and activity patterns) of the patients and controls during five consecutive days and nights. Daily bedtimes were more variable within patients compared with controls (F = 8.19, P < 0.001), but melatonin profiles were equally stable within individuals. Dim‐light melatonin onset was about 1.5 h later in the patient group (U = 771, Z = −4.63, P < 0.001). Patients slept about 1 h less on nights before work days compared with controls (F = 11.21, P = 0.002). The interval between dim‐light melatonin onset and sleep onset was on average 1 h longer in patients compared with controls (U = 1117, Z = −2.62, P = 0.009). This interval was even longer in patients with extremely late chronotype. Melatonin, activity and body temperatures were delayed to comparable degrees in patients. Overall temperatures were lower in patients than controls. Sleep‐onset difficulties correlated with greater distal–proximal temperature gradient (DPG; i.e. colder hands, r2 = −0.32, P = 0.028) in patients. Observed day‐to‐day bedtime variability of individuals with attention‐deficit/hyperactivity disorder and delayed sleep phase syndrome were not reflected in their melatonin profiles. Irregular sleep–wake patterns and delayed sleep in individuals with attention‐deficit/hyperactivity disorder and delayed sleep phase syndrome are associated with delays and dysregulations of the core and skin temperatures.
Once Upon a Time: Parent–Child Dialogue and Storybook Reading in the Electronic Era
Authors: Parish‐Morris Julia , Mahajan Neha , Hirsh‐Pasek Kathy , Golinkoff Roberta Michnick , Collins Molly Fuller 
Published: August 2013

ABSTRACTEarly experiences with books predict later reading success, and an interactive shared reading style called “dialogic reading” is especially beneficial to emergent literacy. Electronic console (EC) books,CD‐rom books, and e‐book apps are designed to teach preschoolers preliteracy skills, but research has yet to systematically explore the impact of these types of books on established predictors of positive literacy outcomes. This research fills that gap with two studies investigating dialogic language and children's story comprehension in a total of 165 parent–child dyads reading battery‐operated, touch‐sensitive children's electronic console books or traditional books. Results revealed that parent–child dialogic reading and children's story comprehension were both negatively affected by the presence of electronic features. Ways in which e‐books may be altered to better serve as educational tools in this new era are discussed.
The impact of microbial symbionts on host plant utilization by herbivorous insects
Authors: Hansen Allison K. , Moran Nancy A. 
Published: August 2013

AbstractHerbivory, defined as feeding on live plant tissues, is characteristic of highly successful and diverse groups of insects and represents an evolutionarily derived mode of feeding. Plants present various nutritional and defensive barriers against herbivory; nevertheless, insects have evolved a diverse array of mechanisms that enable them to feed and develop on live plant tissues. For decades, it has been suggested that insect‐associated microbes may facilitate host plant use, and new molecular methodologies offer the possibility to elucidate such roles. Based on genomic data, specialized feeding on phloem and xylem sap is highly dependent on nutrient provisioning by intracellular symbionts, as exemplified by Buchnera in aphids, although it is unclear whether such symbionts play a substantive role in host plant specificity of their hosts. Microorganisms present in the gut or outside the insect body could provide more functions including digestion of plant polymers and detoxification of plant‐produced toxins. However, the extent of such contributions to insect herbivory remains unclear. We propose that the potential functions of microbial symbionts in facilitating or restricting the use of host plants are constrained by their location (intracellular, gut or environmental), and by the fidelity of their associations with insect host lineages. Studies in the next decade, using molecular methods from environmental microbiology and genomics, will provide a more comprehensive picture of the role of microbial symbionts in insect herbivory.
V. NIH TOOLBOX COGNITION BATTERY (CB): MEASURING WORKING MEMORY
Authors: Tulsky David S. , Carlozzi Noelle E. , Chevalier Nicolas , Espy Kimberly A. , Beaumont Jennifer L. , Mungas Dan 
Published: August 2013

ABSTRACTThis chapter focuses on the NIH Toolbox List Sorting Working Memory Test, which was developed to assess processing speed within the NIH Toolbox Cognition Battery (CB). This test is a sequencing task requiring children and adults to process stimuli (presented both visually and auditorily) and sequence the stimuli according to size. We describe the development of the NIH Toolbox List Sorting Working Memory Test, highlighting its utility in children. We examine descriptive data, test–retest reliability, and convergent and discriminant validity. Results indicated that List Sorting performance was positively correlated with age indicating that performance on the task improved throughout childhood and early adolescence. Further, test–retest reliability coefficients were high and there was support for both convergent and discriminant validity. These data suggest that the NIH Toolbox List Sorting Working Memory Test is reliable and shows evidence of construct validity.
The EATWELL project: Recommendations for healthy eating policy interventions across Europe
Authors: Traill W. B. , Mazzocchi M. , Niedźwiedzka B. , Shankar B. , Wills J. 
Published: August 2013

SummaryThe EATWELL research project (www.eatwellproject.eu) (Interventions to Promote Healthy Eating Habits: Evaluation and Recommendations) funded by the European Commission aimed to improve nutrition policy in Europe by providing scientifically sound evidence on the effectiveness of past healthy eating interventions. This interdisciplinary project involved academic partners from 5 European Union countries and 10 partners in all. As a first step, the project identified 121 large‐scale policy interventions in European countries over the period 1990–2010 and any existing evidence on their evaluation. Of these policies, 82 were aimed at supporting informed choices, and the remaining 39 sought to change the market environment by enhancing the availability of healthier foods, restricting the availability of less healthy foods or nutrients, or changing relative food prices through food taxes or subsidies. Subsequently, advanced quantitative evaluation models were used to conduct a fresh analysis of a small selection of these policy case studies, based on secondary data. Based on these evaluations, together with garnered public and stakeholder opinion, the project put forward proposals for future healthy eating policies, improved evaluation methods and the collection of better data.
The Incidence of Asymptomatic Cerebral Microthromboembolism after Atrial Fibrillation Ablation: Comparison of Warfarin and Dabigatran
Authors: ICHIKI HITOSHI , OKETANI NAOYA , ISHIDA SANEMASA , IRIKI YASUHISA , OKUI HIDEKI , MAENOSONO RYUICHI , NAMINO FUMINORI , NINOMIYA YUICHI , MIYATA MASAAKI , HAMASAKI SHUICHI , TEI CHUWA 
Published: August 2013

BackgroundCerebral microthromboembolism after atrial fibrillation (AF) ablation has been reported in 4–20% with perioperative warfarin. Dabigatran is a new anticoagulant in patients with nonvalvular AF. We investigated the incidence of asymptomatic cerebral microthromboembolism after AF ablation with perioperative warfarin or dabigatran using diffusion‐weighted and T2‐weighted magnetic resonance imaging (MRI).Methods and ResultsOur study included 210 consecutive patients with AF (111 paroxysmal and 99 persistent) who underwent complex fractionated atrial electrogram‐guided ablation (combined with pulmonary vein isolation, n = 110). Catheter irrigation was performed in all cases. Uninterrupted warfarin therapy was used in 180 patients (warfarin group) and interrupted only on the morning of the procedure with dabigatran in 30 (dabigatran group). All patients underwent cerebral MRI the day after ablation. New microthromboemboli were detected in 10.0% of the warfarin group and 26.7% of the dabigatran group (P < 0.05). The incidence of hemopericardium treated with pericardiocentesis was lower in the warfarin group than in the dabigatran group (2.5% vs 11.1%, P < 0.05). In multivariate analysis, the use of cardioversion was a predictor of new microthromboembolism development after AF ablation.ConclusionsThe incidence of asymptomatic cerebral microthromboembolism and hemopericardium after AF ablation was significantly lower with perioperative warfarin therapy than with dabigatran therapy. Dabigatran may not be an effective alternative to warfarin for AF ablation, especially in patients who undergo cardioversion.
Current situation and characterization ofPseudomonas syringaepv.actinidiaeon kiwifruit inGalicia (northwestSpain)
Authors: Abelleira A. , Ares A. , Aguín O. , Picoaga A. , López M. M. , Mansilla P. 
Published: August 2013

Bacterial canker of kiwifruit, caused byPseudomonas syringaepv. actinidiae(Psa), is a disease that is spreading rapidly in several kiwifruit‐producing countries, causing significant economic losses. In 2011, it was detected for the first time inSpain, in the south ofGalicia (northwestSpain). Kiwifruit orchards were therefore inspected and sampled in 2011 and 2012 to determine the pathogen distribution, and the isolates obtained were characterized by morphology, fatty acids profile, biochemical tests and molecular techniques. Isolates were obtained fromActinidia deliciosa‘Hayward’ (from leaves, canes, flower buds, fruits and roots), fromA. deliciosa‘Summer’, fromActinidia chinensis‘JinTao’ (from canes and leaves) and fromA. chinensispollinator ‘Belén’ (from canes). Results of the analysis of thecflgene (phytotoxin production‐related), thetox–argKgene cluster and phylogenetic analysis of thectsgene demonstrated that allPsa isolates from northwestSpain correspond to thePsa3 population, which includes strains of haplotype 2. This is the first record ofPsa3 and haplotype 2 inSpain.
Draft Genome Sequence of Klebsiella pneumoniae Isolate PR04
Authors: Zulkifli M. H. , Teh L. K. , Lee L. S. , Zakaria Z. A. , Salleh M. Z. 
Published: August 2013

ABSTRACT

Klebsiella pneumoniae
PR04 was isolated from a patient hospitalized in Malaysia. The draft genome sequence of
K. pneumoniae
PR04 shows differences compared to the reference sequences of
K. pneumoniae
strains MGH 78578 and NTUH-K2044 in terms of their genomic structures.
Draft Genome Sequence of Cyclobacterium qasimii Strain M12-11B
T
, Isolated from Arctic Marine Sediment
Authors: Shivaji S. , Ara Sreenivas , Singh Aditya , Kumar Pinnaka Anil 
Published: August 2013

ABSTRACT

A 6.29-Mb genome sequence of
Cyclobacterium qasimii
strain M12-11B
T
, isolated from an Arctic marine sediment sample, is reported.
A qualitative study comparing experiences of the surgical safety checklist in hospitals in high-income and low-income countries
Authors: Aveling Emma-Louise , McCulloch Peter , Dixon-Woods Mary 
Published: August 2013

ObjectiveBold claims have been made for the ability of the WHO surgical checklist to reduce surgical morbidity and mortality and improve patient safety regardless of the setting. Little is known about how far the challenges faced by low-income countries are the same as those in high-income countries or different. We aimed to identify and compare the influences on checklist implementation and compliance in the UK and Africa.DesignEthnographic study involving observations, interviews and collection of documents. Thematic analysis of the data.SettingOperating theatres in one African university hospital and two UK university hospitals.Participants112 h of observations were undertaken. Interviews with 39 theatre and administrative staff were conducted.ResultsMany staff saw value in the checklist in the UK and African hospitals. Some resentment was present in all settings, linked to conflicts between the philosophy behind the checklist and the realities of local cultural, social and economic contexts. Compliance—involving use, completeness and fidelity—was considerably higher, though not perfect, in the UK settings. In these hospitals, compliance was supported by established structures and systems, and was not significantly undermined by major resource constraints; the same was not true of the low-income context. Hierarchical relationships were a major barrier to implementation in all settings, but were more marked in the low-income setting. Introducing a checklist in a professional environment characterised by a lack of accountability and transparency could make the staff feel jeopardised legally, professionally, and personally, and it encouraged them to make misleading records of what had actually been done.ConclusionsSurgical checklist implementation is likely to be optimised, regardless of the setting, when used as a tool in multifaceted cultural and organisational programmes to strengthen patient safety. It cannot be assumed that the introduction of a checklist will automatically lead to improved communication and clinical processes.
MGMT-STP27 Methylation Status as Predictive Marker for Response to PCV in Anaplastic Oligodendrogliomas and Oligoastrocytomas. A Report from EORTC Study 26951
Authors: van den Bent Martin J. , Erdem-Eraslan Lale , Idbaih Ahmed , de Rooi Johan , Eilers Paul H.C. , Spliet Wim G.M. , den Dunnen Wilfred F.A. , Tijssen Cees , Wesseling Pieter , Sillevis Smitt Peter A.E. , Kros Johan M. , Gorlia Thierry , French Pim J. 
Published: August 2013

Abstract
Purpose: The long-term follow-up results from the EORTC-26951 trial showed that the addition of procarbazine, CCNU, and vincristine (PCV) after radiotherapy increases survival in anaplastic oligodendrogliomas/oligoastrocytomas (AOD/AOA). However, some patients appeared to benefit more from PCV treatment than others.
Experimental Design: We conducted genome-wide methylation profiling of 115 samples included in the EORTC-26951 trial and extracted the CpG island hypermethylated phenotype (CIMP) and MGMT promoter methylation (MGMT-STP27) status.
Results: We first show that methylation profiling can be conducted on archival tissues with a performance that is similar to snap-frozen tissue samples. We then conducted methylation profiling on EORTC-26951 clinical trial samples. Univariate analysis indicated that CIMP+ or MGMT-STP27 methylated tumors had an improved survival compared with CIMP− and/or MGMT-STP27 unmethylated tumors [median overall survival (OS), 1.05 vs. 6.46 years and 1.06 vs. 3.8 years, both P &lt; 0.0001 for CIMP and MGMT-STP27 status, respectively]. Multivariable analysis indicates that CIMP and MGMT-STP27 are significant prognostic factors for survival in presence of age, sex, performance score, and review diagnosis in the model. CIMP+ and MGMT-STP27 methylated tumors showed a clear benefit from adjuvant PCV chemotherapy: the median OS of CIMP+ samples in the RT and RT-PCV arms was 3.27 and 9.51 years, respectively (P = 0.0033); for MGMT-STP27 methylated samples, it was 1.98 and 8.65 years. There was no such benefit for CIMP- or for MGMT-STP27 unmethylated tumors. MGMT-STP27 status remained significant in an interaction test (P = 0.003). Statistical analysis of microarray (SAM) identified 259 novel CpGs associated with treatment response.
Conclusions: MGMT-STP27 may be used to guide treatment decisions in this tumor type. Clin Cancer Res; 19(19); 5513–22. ©2013 AACR.
Clinical Response to a Lapatinib-Based Therapy for a Li-Fraumeni Syndrome Patient with a Novel HER2V659E Mutation
Authors: Serra Violeta , Vivancos Ana , Puente Xose S. , Felip Enriqueta , Silberschmidt Daniel , Caratù Ginevra , Parra Josep-Lluís , De Mattos-Arruda Leticia , Grueso Judit , Hernández-Losa Javier , Arribas Joaquín , Prudkin Ludmila , Nuciforo Paolo , Scaltriti Maurizio , Seoane Joan , Baselga José 
Published: August 2013

Abstract
Genomic characterization of recurrent breast and lung tumors developed over the course of 10 years in a 29-year-old patient with a germline TP53 mutation (Li-Fraumeni Syndrome) identified oncogenic alterations in the HER2 and EGFR genes across all tumors, including HER2 amplifications, an EGFR-exon 20 insertion, and the first-in-humans HER2V659E mutation showing a phenotypic convergent evolution toward HER2 and EGFR alterations. Following the identification of HER2-activating events in the most recent lung carcinoma and in circulating tumor cells, we treated the reminiscent metastatic lesions with a lapatinib-based therapy. A symptomatic and radiologic clinical response was achieved. HER2V659E sensitivity to lapatinib was confirmed in the laboratory.
Significance: The precise knowledge of the genomic alterations present in tumors is critical to selecting the optimal treatment for each patient. Here, we report the molecular characterization and clinical response to a lapatinib-based therapy for the tumors of a Li-Fraumeni patient showing prevalence of HER2 and EGFR genomic alterations. Cancer Discov; 3(11); 1238–44. ©2013 AACR.
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Endothelial G Protein–Coupled Receptor Kinase 2 Regulates Vascular Homeostasis Through the Control of Free Radical Oxygen Species
Authors: Ciccarelli Michele , Sorriento Daniela , Franco Antonietta , Fusco Anna , Del Giudice Carmine , Annunziata Roberto , Cipolletta Ersilia , Monti Maria Gaia , Dorn Gerald W. , Trimarco Bruno , Iaccarino Guido 
Published: August 2013

Objective—

The role of endothelial G protein–coupled receptor kinase 2 (GRK2) was investigated in mice with selective deletion of the kinase in the endothelium (Tie2-CRE/GRK2
fl/fl
).

Approach and Results—

Aortas from Tie2-CRE/GRK2
fl/fl
presented functional and structural alterations as compared with control GRK2
fl/fl
mice. In particular, vasoconstriction was blunted to different agonists, and collagen and elastic rearrangement and macrophage infiltration were observed. In primary cultured endothelial cells deficient for GRK2, mitochondrial reactive oxygen species was increased, leading to expression of cytokines. Chronic treatment with a reactive oxygen species scavenger in mice corrected the vascular phenotype by recovering vasoconstriction, structural abnormalities, and reducing macrophage infiltration.

Conclusions—
These results demonstrate that GRK2 removal compromises vascular phenotype and integrity by increasing endothelial reactive oxygen species production.