bluuebunny/crossref_metadata_2025_split huggingface dataset:
OxLDL as a prognostic biomarker of plaque instability in patients qualified for carotid endarterectomy
Authors: Woźniak Agnieszka , Satała Joanna , Gorzelak‐Pabiś Paulina , Pawlos Agnieszka , Broncel Marlena , Kaźmierski Piotr , Woźniak Ewelina 
Published: July 2024

AbstractAtherosclerotic plaque instability increases the risk of stroke. As such, determining the nature of an instability atherosclerotic plaque may speed up qualification for carotid endarterectomy (CEA), thus reducing the risk of acute vascular events. The aim of the study was to determine the diagnostic value of oxidized LDL cholesterol (ox‐LDL), matrix metalloproteinase 9 (MMP‐9) and 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG) in serum as a prognostic markers of instability atherosclerotic plaques. Serum was collected from 67 patients who underwent CEA in accordance with the qualification criteria. The levels of ox‐LDL, MMP‐9 and 8‐OHdG were assessed by ELISA. The predictive value of the markers was determined based on an ROC curve, and the cut‐off points with the highest sensitivity and specificity were determined. Patients with unstable atherosclerotic plaque had significantly higher serum ox‐LDL, MMP‐9 and 8‐OHdG values. It was found that in patients before CEA, ox‐LDL >31.4 ng/mL was associated with an 82.5% probability of unstable atherosclerotic plaque, MMP‐9 >113.1 ng/mL with 78.6%, and 8‐OHdG >2.15 ng/mL with 64.7%. Multivariate regression analysis found ox‐LDL to be an independent factor associated with plaque instability. Patients with unstable plaques tend to have higher serum levels of ox‐LDL, MMP‐9 and 8‐OHdG compared to those with stable plaques. The optimal cut‐off point for ox‐LDL (AUC 0.86, p <0.0001) was 31.14 ng/mL, with 91.18% sensitivity and 78.79% specificity. The high sensitivity and specificity of ox‐LDL suggests that it can be used as an independent marker of plaque instability.
Couple and family optimal experiences: Integrating flow theory into the relational sciences
Authors: Somjee Ali Asad 
Published: July 2024

AbstractShared experiences are a fundamental aspect of couple and family life that impact both individual well‐being and satisfaction with close relationships. Optimal experience, also known as flow, is the state of complete absorption and fulfillment experienced when engaged in an intrinsically rewarding activity. The interdisciplinary concept of flow provides a sound basis to explore optimal shared experiences. Research on flow experiences in couple and family contexts is limited, presenting a gap in the literature. This article outlines a research program integrating flow theory into the relational sciences along three lines of inquiry. First, couple/family flow requires a clear definition and theoretical framework by building upon previous scholarly work on social flow. Second, the impact of flow experiences on relational variables (e.g., relationship quality, interpersonal commitment, sexual satisfaction) requires investigation. Lastly, flow theory presents an opportunity to develop new interventions aimed at improving the experiences couples and families share together.
The actin motor protein OsMYA1 associates with OsExo70H1 and contributes to rice secretory defense by modulating OsSyp121 distribution
Authors: Li Yuan‐Bao , Liu Chengyu , Shen Ningning , Zhu Shuai , Deng Xianya , Liu Zixuan , Han Li‐Bo , Tang Dingzhong 
Published: July 2024

ABSTRACTMagnaporthe oryzae (M. oryzae) is a devastating hemibiotrophic pathogen. Its biotrophic invasive hyphae (IH) are enclosed in the extrainvasive hyphal membrane produced by plant cells, thus generating a front line of the battlefield between the pathogen and the host plants. In plants, defense‐related complexes such as proteins, callose‐rich materials and vesicles, are directionally secreted to this interface to confer defense responses, but the underlying molecular mechanism is poorly understood. In this study, we found that a Myosin gene, Myosin A1 (OsMYA1), contributed to rice defense. The OsMYA1 knockout mutant exhibited decreased resistance to M. oryzae infection. OsMYA1 localizes to the actin cytoskeleton and surrounds the IH of M. oryzae. OsMYA1 interacts with an exocyst subunit, OsExo70H1, and regulates its accumulation at the plasma membrane (PM) and pathogen–plant interface. Furthermore, OsExo70H1 interacted with the rice syntaxin of the plants121 protein (OsSyp121), and the distribution of OsSyp121 to the PM or the pathogen–plant interface was disrupted in both the OsMYA1 and OsExo70H1 mutants. Overall, these results not only reveal a new function of OsMYA1 in rice blast resistance, but also uncover a molecular mechanism by which plants regulate defense against M. oryzae by OsMYA1‐initiated vesicle secretory pathway, which originates from the actin cytoskeleton to the PM.
BBX9 forms feedback loops with PIFs and BBX21 to promote photomorphogenic development
Authors: Song Zhaoqing , Ye Wanying , Jiang Qing , Lin Huan , Hu Qing , Xiao Yuntao , Bian Yeting , Zhao Fengyue , Dong Jie , Xu Dongqing 
Published: July 2024

ABSTRACTLight is one of the most essential environmental factors that tightly and precisely control various physiological and developmental processes in plants. B‐box CONTAINING PROTEINs (BBXs) play central roles in the regulation of light‐dependent development. In this study, we report that BBX9 is a positive regulator of light signaling. BBX9 interacts with the red light photoreceptor PHYTOCHROME B (phyB) and transcription factors PHYTOCHROME‐INTERACTING FACTORs (PIFs). phyB promotes the stabilization of BBX9 in light, while BBX9 inhibits the transcriptional activation activity of PIFs. In turn, PIFs directly bind to the promoter of BBX9 to repress its transcription. On the other hand, BBX9 associates with the positive regulator of light signaling, BBX21, and enhances its biochemical activity. BBX21 associates with the promoter regions of BBX9 and transcriptionally up‐regulates its expression. Collectively, this study unveiled that BBX9 forms a negative feedback loop with PIFs and a positive one with BBX21 to ensure that plants adapt to fluctuating light conditions.
Use of distinct projective techniques in investigating participants' perception of a clean label dairy product: A study on the presence of additives/stabilizers in Ultra high temperature processing milk's label in Brazil
Authors: de Alcântara Natália Emmerick , Tavares Filho Elson Rogério , Pimentel Tatiana Colombo , Pagani Mônica Marques , Mársico Eliane Teixeira , da Cruz Adriano Gomes , Esmerino Erick Almeida 
Published: July 2024

AbstractUsing an online questionnaire, the perception of additives/stabilizers in ultra‐high temperature (UHT) milk was investigated using different projective techniques. Clustering data on the Food Choice Questionnaire (FCQ) identified two groups of participants, Moderates and Judicious, with the latter assigning significantly higher agreement scores for all FCQ items. In the Word Association task, clean label UHT milk was more associated with “Nutrients and Constituents” by the Moderates and less by the Judicious, suggesting that for part of the interviewed participants, the absence of stabilizers increases the general quality of the product. In the Product Personality Profiling task, the same product was related to “young people” and a “balanced diet” by the Moderates. At the same time, the Judicious was designed for a stereotype of healthy living. Finally, the methodologies used provide valuable insights into the dairy sector, holistically highlighting the nuances in consumer preferences and expectations, offering a significant strategic opportunity for developing and promoting new clean‐label products in the segment.Practical ApplicationInvestigating consumer perceptions regarding the absence of additives offers valuable insights for marketing strategies and product development aimed at different consumer market segments, especially the dairy sector, and contributes to consumer psychology and behavioral research. At the same time, the food industry can use these findings to improve attribute communication, build consumer confidence, and adapt products to identified preferences, reflecting a practical intersection between academia and the industry.
Understanding food consumer behavior using projective methods and the big five personality traits model: The case of traditional Mexican cheeses
Authors: Rojas‐Rivas Edgar , Thomé‐Ortiz Humberto , Martínez‐García Carlos Galdino , Salgado‐Beltrán Lizbeth 
Published: July 2024

AbstractThis research aimed to use two projective methods and the Big Five Personality Traits (BFPT) model to study food consumer behavior in the case of traditional Mexican cheeses. Based on an online survey (n = 197), this study employs the Word Association and Mason‐Haire's Shopping List projective techniques with two shopping lists (traditional vs. supermarket). Several factors influencing consumers' perceptions of traditional cheeses were identified, such as hedonism, sensory characteristics, quality and freshness. The shopping list allowed the identification of factors related to food consumption and consumer personality traits. Conscientiousness and Neuroticism were associated with the profile of the consumer who purchases food in supermarkets. In contrast, the personalities of Agreeableness and Openness were associated with the profile of the consumer of traditional Mexican cheeses. The results of this research could be helpful in promoting and marketing traditional foods, considering consumers' personality traits.Practical applicationsThis research identified consumer perceptions of traditional cheeses produced in the central region of Mexico and the personality traits associated with the consumer profile of these food products. The results can be of great use to the cheese‐producing agroindustry, considering that consumers are aware that by purchasing these products, they are contributing to the development and support of the local economy. Therefore, it is essential for producers and sellers to consider the attributes that consumers associate with traditional cheeses, such as artisanal and local characteristics, hedonic value, and consumers' personality traits.
Elevational shifts in tree community composition in the Brazilian Atlantic Forest related to climate change
Authors: Bergamin Rodrigo Scarton , Bastazini Vinicius Augusto Galvão , Esquivel‐Muelbert Adriane , Bordin Kauane Maiara , Klipel Joice , Debastiani Vanderlei Júlio , Vibrans Alexander Christian , Loyola Rafael , Müller Sandra Cristina 
Published: July 2024

AbstractQuestionClimate change induces shifts in species distributions, ultimately changing community composition. Mountains are especially sensitive to climate change, and tree species are predicted to move towards higher elevations, but observed changes are not always unidirectional. The diversity and complexity of tropical and subtropical systems limits our understanding of climate‐induced responses of subtropical mountain forests. Here, we investigated migration trends in tree community composition along an elevational gradient, and between the transition from lowland to montane forests in subtropical forests.LocationBrazilian Atlantic Forest.MethodsWe used thermal affiliations of 627 tree species to calculate community temperature scores (CTS) for different life‐history stages of trees in 96 permanent plots. We compared CTS of different life‐history stages across space and time.ResultsMost tree communities (58%) did not show a significant difference of CTS between life‐history stages, indicating a non‐migration trend. On the other hand, 27% of tree communities showed upward migration and 15% downward migration. Upward migration was more common in montane forests, and downward migration in lowland forests. Our temporal analysis shows significant changes in CTS values for juvenile communities with 0.36°C decrease in lowland forests and 0.34°C increase in montane forests.ConclusionsContrasting results between lowland and montane forest communities indicate that the transition zone influences migration patterns and may reflect differences in species’ thermal limitations, as well as by non‐thermal factors such as biotic interactions. Our findings provide the first evidence of climate change‐induced community shifts in the Brazilian Atlantic Forest. We demonstrated that upward migration trends were predominantly observed in montane–upper mountain forests, while downward migrations were noted in lowland–submontane forests. The thermophilization of montane forests may suggest an increased risk of reduction for cold‐demanding species under climate change scenarios.
Ultraviolet attenuates centromere‐mediated meiotic genome stability and alters gametophytic ploidy consistency in flowering plants
Authors: Fu Huiqi , Zhong Jiaqi , Zhao Jiayi , Huo Li , Wang Chong , Ma Dexuan , Pan Wenjing , Sun Limin , Ren Ziming , Fan Tianyi , Wang Ze , Wang Wenyi , Lei Xiaoning , Yu Guanghui , Li Jing , Zhu Yan , Geelen Danny , Liu Bing 
Published: July 2024

Summary

Ultraviolet (UV) radiation influences development and genome stability in organisms; however, its impact on meiosis, a special cell division essential for the delivery of genetic information across generations in eukaryotes, has not yet been elucidated.

In this study, by performing cytogenetic studies, we reported that UV radiation does not damage meiotic chromosome integrity but attenuates centromere‐mediated chromosome stability and induces unreduced gametes in Arabidopsis thaliana.

We showed that functional centromere‐specific histone 3 (CENH3) is required for obligate crossover formation and plays a role in the protection of sister chromatid cohesion under UV stress. Moreover, we found that UV specifically alters the orientation and organization of spindles and phragmoplasts at meiosis II, resulting in meiotic restitution and unreduced gametes. We determined that UV‐induced meiotic restitution does not rely on the UV Resistance Locus8‐mediated UV perception and the Tapetal Development and Function1‐ and Aborted Microspores‐dependent tapetum development, but possibly occurs via altered JASON function and downregulated Parallel Spindle1.

This study provides evidence that UV radiation influences meiotic genome stability and gametophytic ploidy consistency in flowering plants.
Mereological Nihilism and Material Constitution
Authors: Thunder Simon 
Published: July 2024

AbstractMereological nihilists typically employ a paraphrase strategy in order to mitigate the apparent absurdity of their denial of the existence of composite objects. I argue here that the nihilist's paraphrase strategy is incomplete, because no schema for generating nihilistically acceptable paraphrases of sentences concerning material constitution has ever been given. Nor can an adequate schema be arrived at by generalising things that nihilists have already said. I fill this lacuna in the nihilist's account by developing and defending a novel paraphrase schema that makes essential reference to the determinable‐determinate relation that holds between arrangement‐properties instantiated by the simples.
A stage‐dependent seed defense response to explain efficient seed transmission of Xanthomonas citri pv. fuscans to common bean
Authors: Darrasse Armelle , Tarkowski Łukasz Paweł , Briand Martial , Lalanne David , Chen Nicolas W. G. , Barret Matthieu , Verdier Jerome 
Published: July 2024

AbstractAlthough seed represents an important means of plant pathogen dispersion, the seed–pathogen dialogue remains largely unexplored. A multiomic approach was performed at different seed developmental stages of common bean (Phaseolus vulgaris L.) during asymptomatic colonization by Xanthomonas citri pv. fuscans (Xcf), At the early seed developmental stages, we observed high transcriptional changes both in seeds with bacterial recognition and defense signal transduction genes, and in bacteria with up‐regulation of the bacterial type 3 secretion system. This high transcriptional activity of defense genes in Xcf‐colonized seeds during maturation refutes the widely diffused assumption considering seeds as passive carriers of microbes. At later seed maturation stages, few transcriptome changes indicated a less intense molecular dialogue between the host and the pathogen, but marked by changes in DNA methylation of plant defense genes, in response to Xcf colonization. We showed examples of pathogen‐specific DNA methylations in colonized seeds acting as plant defense silencing to repress plant immune response during the germination process. Finally, we propose a novel plant–pathogen interaction model, specific to the seed tissues, highlighting the existence of distinct phases during seed–pathogen interaction with seeds being actively interacting with colonizing pathogens, then both belligerents switching to more passive mode at later stages.
Discrimination of relatedness drives rice flowering and reproduction in cultivar mixtures
Authors: Xu You , Li Feng‐Li , Li Lei‐Lei , Chen Xin , Meiners Scott J. , Kong Chui‐Hua 
Published: July 2024

AbstractThe improvement of performance and yield in both cultivar and species mixtures has been well established. Despite the clear benefits of crop mixtures to agriculture, identifying the critical mechanisms behind performance increases are largely lacking. We experimentally demonstrated that the benefits of rice cultivar mixtures were linked to relatedness‐mediated intraspecific neighbour recognition and discrimination under both field and controlled conditions. We then tested biochemical mechanisms of responses in incubation experiments involving the addition of root exudates and a root‐secreted signal, (–)‐loliolide, followed by transcriptome analysis. We found that closely related cultivar mixtures increased grain yields by modifying root behaviour and accelerating flowering over distantly related mixtures. Importantly, these responses were accompanied by altered concentration of signalling (–)‐loliolide that affected rice transcriptome profiling, directly regulating root growth and flowering gene expression. These findings suggest that beneficial crop combinations may be generated a‐priori by manipulating neighbour genetic relatedness in rice cultivar mixtures and that root‐secreted (–)‐loliolide functions as a key mediator of genetic relatedness interactions. The ability of relatedness discrimination to regulate rice flowering and yield raises an intriguing possibility to increase crop production.
Arachidonic acid‐derived dihydroxy fatty acids in neonatal cord blood relate symptoms of autism spectrum disorders and social adaptive functioning: Hamamatsu Birth Cohort for Mothers and Children (HBC Study)
Authors: Hirai Takaharu , Umeda Naoko , Harada Taeko , Okumura Akemi , Nakayasu Chikako , Ohto‐Nakanishi Takayo , Tsuchiya Kenji J. , Nishimura Tomoko , Matsuzaki Hideo 
Published: July 2024

AimAutism spectrum disorder (ASD) is associated with abnormal lipid metabolism, such as a high total ratio of omega‐6 to omega‐3 in polyunsaturated fatty acids (PUFAs). PUFAs are metabolized to epoxy fatty acids by cytochrome P450 (CYP); then, dihydroxy fatty acid is produced by soluble epoxide hydrolase. This study examined the association between PUFA metabolites in the cord blood and ASD symptoms and adaptive functioning in children.MethodsThis prospective cohort study utilized cord blood to quantify PUFA metabolites of the CYP pathway. The Autism Diagnostic Observation Schedule (ADOS‐2) and Vineland Adaptive Behaviors Scales, Second Edition (VABS‐II) were used to assess subsequent ASD symptoms and adaptive functioning in children at 6 years. The analysis included 200 children and their mothers.ResultsArachidonic acid‐derived diols, 11,12‐diHETrE was found to impact ASD symptom severity on the ADOS‐2‐calibrated severity scores and impairment in the socialization domain as assessed by the VABS‐II (P = 0.0003; P = 0.004, respectively). High levels of 11,12‐diHETrE impact social affect in ASD symptoms (P = 0.002), while low levels of 8,9‐diHETrE impact repetitive/restrictive behavior (P = 0.003). Notably, there was specificity in the association between diHETrE and ASD symptoms, especially in girls.ConclusionThese findings suggest that the dynamics of diHETrE during the fetal period is important in the developmental trajectory of children after birth. Given that the role of diol metabolites in neurodevelopment in vivo is completely uncharacterized, the results of this study provide important insight into the role of diHETrE and ASD pathophysiology.
C4 monocots and C4 dicots exhibit rapid photosynthetic induction response in contrast to C3 plants
Authors: Tanigawa Keiichiro , Yuchen Qu , Katsuhama Naoya , Sakoda Kazuma , Wakabayashi Yu , Tanaka Yu , Sage Rowan , Lawson Tracy , Yamori Wataru 
Published: July 2024

AbstractConsidering the prevalence of ever‐changing conditions in the natural world, investigation of photosynthetic responses in C4 plants under fluctuating light is needed. Here, we studied the effect of dynamic illumination on photosynthesis in totally 10 C3, C3–C4 intermediate, C4‐like and C4 dicots and monocots at CO2 concentrations of 400 and 800 μmol mol−1. C4 and C4‐like plants had faster photosynthetic induction and light‐induced stomatal dynamics than C3 plants at 400 μmol mol−1, but not at 800 μmol mol−1 CO2, at which the CO2 supply rarely limits photosynthesis. C4 and C4‐like plants had a higher water use efficiency than C3 plants at both CO2 concentrations. There were positive correlations between photosynthetic induction and light‐induced stomatal response, together with CO2 compensation point, which was a parameter of the CO2‐concentrating mechanism of C4 photosynthesis. These results clearly show that C4 photosynthesis in both monocots and dicots adapts to fluctuating light conditions more efficiently than C3 photosynthesis. The rapid photosynthetic induction response in C4 plants can be attributed to the rapid stomatal dynamics, the CO2‐concentrating mechanism or both.
Development of Four‐Item Attitudes toward People Living with Dementia Scale for population surveys
Authors: Matsumoto Hiroshige , Takaoka Manami , Yamamoto‐Mitani Noriko , Igarashi Ayumi 
Published: July 2024

AbstractBackgroundThis study developed a short version of a scale measuring attitudes toward people living with dementia, the Four‐Item Attitudes toward People Living with Dementia Scale (APDS4), that could be included in a large population survey.MethodsWe used three datasets from Japan: a web panel survey, a community‐based mail survey, and data from a randomised controlled trial on dementia education. The original scale used was the Attitudes toward People Living with Dementia Scale developed by Kim and Kuroda. Test–retest reliability and item response theory analyses were used to reduce the number of items. The reliability, internal consistency, validity, and responsiveness of the short version were evaluated.ResultsSix items with low test–retest reliability and four items with low discrimination parameters were removed from the 14‐item scale. The APDS4, consisting of four items, had test–retest reliability and internal consistency comparable to those of the original scale. Confirmatory factor analysis indicated that the APDS4 fit a unidimensional model. The validity of the APDS4 was confirmed by significant associations between the APDS4 scores and the original scale scores, knowledge of dementia, helping behaviour intentions toward people living with dementia, helping behaviour experience, attending the Dementia Supporter Training Course, and engagement in healthcare jobs. In a randomised controlled trial dataset, the APDS4 was more responsive to educational interventions than the original scale.ConclusionThe shortened APDS4 was established as a reliable, validated, and responsive scale. This scale can be used efficiently in population surveys to evaluate dementia‐friendly initiatives at the community level.