bluuebunny/crossref_metadata_2025_split huggingface dataset:
Junctional adhesion molecular 3 (JAM3) is a novel tumor suppressor and improves the prognosis in breast cancer brain metastasis via the TGF-β/Smad signal pathway
Authors: Zhu Kaitao , Li Shiwei , Yao Hongru , Hei Jilong , Martin Tracey , Zhang Shanyi 
Published: August 2024

Abstract

Purpose
The incidence of breast cancer brain metastasis (BCBM) is a deadly clinical problem, and exact mechanisms remain elusive. Junction adhesion molecule (JAM), a tight junction protein, is a key negative regulator of cancer cell invasion and metastasis.
Methods
Junction adhesion molecular 3 (JAM3) expression in breast cancer was analyzed by bioinformatics method and confirmed by PCR, western blot, and immunofluorescence (IF) in cell lines. The effect of exogenous expression of JAM3 through lentivirus vectors on invasion, adhesion, and apoptosis was verified using transwell assay and flow cytometer. Differentially expressed genes (DEGs) were detected by RNA sequence and verified by q-PCR and Western bot. The effect of silencing JAM3 using siRNA was assessed by adhesion assay. Kaplan-Meier analysis was applied to calculate the impact of JAM3 expression and classic clinicopathologic characteristics on survival.
Results
Bioinformatics analysis revealed that JAM3 expression was reduced in BCBM. Exogenous expression of JAM3 minimizes the ability to invade, adhesion and promotes apoptosis of breast cancer cells. Silencing JAM3 results in morphology-changing and recovering invasion and adhesion to ECMs and the TGF-β/Smad signal pathway may be involved. JAM3 predicts less metastasis and good survival in patients with BCBM. Statistical analysis examined the correlation between JAM3 expression in BCBM samples detected by IHC and the clinicopathological characteristics. Kaplan-Meier analysis indicated that a high expression level of JAM3 was associated with longer survival time.
Conclusion
JAM3 can serve as a key negative regulator of breast cancer cell invasion, apoptosis, and brain metastasis, which may be linked to the TGF/Smad signal pathway. JAM3 has been anticipated to be a promising biomarker in the diagnosis and prognosis of breast cancer.
Based on pN-stage to Identify Gastric Carcinoma Patients with Overall Survival Benefit from Postoperative Radiotherapy: A Propensity Score Matching Analysis and Subgroup Analysis
Authors: Wan Guangmin , Wang Gang , Yang Lu , Xu Gang 
Published: August 2024

Abstract

Background: It is currently controversial whether regional lymph node status is related to the benefit of postoperative radiotherapy for gastric cancer.
Methods: All surgically treated patients with more than 15 lymph nodes examined diagnosed by positive histology between January 2007–December 2019 were included from the SEER database. All the patients receiving adjuvant chemotherapy were subsequently compared by propensity score matching (PSM), with overall survival (OS) as the endpoint, adjuvant radiotherapy (ART) as the only variable. Results of subgroup analyses are presented in a forest plot.
Results: There are 1882 patients included in this study, 1301(69.1%) patients in the ART group and 581(30.9%) patients in the no-ART group. After PSM, each group included 573 patients. Overall, ART did not significantly improve 3-year overall survival (OS; 55.5% vs 51.1%, p = 0.07). However, in the subgroup of patients with pathologic lymph node metastasis (pN3-stage), 55-70 years old, tumor size > 89mm and T3-stage benefited from postoperative radiotherapy (p < 0.05). We further performed PSM and survival analysis on total patients with different pathological lymph node stages to validate the role of ART. We statistically observed survival benefits in pN+ (positive pathologic lymph node) and pN3 groups, but no similar effects were observed in pN1 and pN2 groups. For pN0 groups, although the results showed no statistical significance, we believe that ART should not be performed.
Conclusion: ART significantly improved prognosis in the patients with pN3-stage. More researches are needed to further validate the role of ART for gastric cancer.
Prognostic value of prostate volume and nomograms for predicting recurrence in patients with non-muscle invasive bladder cancer: a multi-institutional study.
Authors: Hu Dichao , Liu He , Li Mingyang , Wu Wenbo , Ma Chenxu , Chen Lujie , Lin Yunqiao , Li Peng , Du Yiheng , Shao Zhiqiang , Qiao Liang , Wang Yongchuan , Liu HaiTao 
Published: August 2024

Abstract

Purpose
We conducted an assessment to investigate the impact of prostate volume on the recurrence of patients with non-muscle invasive bladder cancer (NMIBC). Subsequently, we developed and validated nomograms to accurately evaluate recurrence in NMIBC patients. Additionally, we examined the potential improvement in predictive capability achieved by introducing prostate volume as a variable in the model.
Methods
We conducted a retrospective analysis, enrolling 555 eligible patients from seven independent medical institutions across China. We first evaluate recurrence-free survival outcomes in patients with varying prostate volumes. Subsequently, we divided patients into a training cohort and an external validation cohort. Univariate and multivariate Cox regression analyses were conducted within the training cohort. Accordingly, two nomogram models with and without prostate volumes were developed. Their performance was compared by concordance index, calibration curves, receiver operating characteristics curves, and decision curve analysis. Furthermore, a risk classification model utilizing the nomogram incorporating prostate volume was developed.
Results
The 3-year recurrence-free survival was markedly lower in patients with large prostate volumes (> 30 ml) compared to those with relatively small prostate volumes (< 30 ml) (p < 0.001). The AUC for the model incorporating prostate volume at 3 years in the training cohort and external validation cohort was 0.803 and 0.776, surpassing the AUC for the model excluding prostate volume at the corresponding intervals, which was 0.787 and 0.767. The 1- and 2-year AUC for the two models also exhibited similar differences. The decision curve analysis results demonstrated the significant superiority of the nomogram incorporating prostate volume over the one without it.
Conclusion
Our investigation revealed that prostate volume significantly influences recurrence in patients with NMIBC. We successfully developed a more accurate nomogram by introducing prostate volume as a variable and provided new insights to further guide clinical management and individualized treatment of NMIBC patients.
Comparision of Intravaginal with Interstitial Brachytherapy in Cervical Cancer after Inadvertent Hysterectomy: A Retrospective Study
Authors: Choudhary Sunil , Pandey Ankita , Singh Ankita , Mourya Ankur , Gupta Neha , Shajid Syed Mohamed , Venkatachalam Gogul Priean , Rai Sangita , Agarwal Sakshi , Venkatraman Pitchaikannu , Aggarwal Lalit Mohan 
Published: August 2024

Abstract

Purpose
The purpose of this study was to compare intravaginal (IVBT) with interstitial brachytherapy (ISBT) in patients presenting with residual or recurrent disease after inadvertent hysterectomy for cervical cancer.
Methods
Records of consecutive patients with cervical cancer, registered in the Radiotherapy Outpatient Department (RTOPD) between March 2018 and March 2021, who had inadvertent hysterectomy followed by external beam radiotherapy (EBRT) and IVBT (n = 15) or ISBT (n = 15) were analyzed retrospectively. All the patients received a dose of 45Gy-50Gy/25 fractions in 5 weeks by EBRT with concurrent cisplatin. A dose of 6Gy/fraction/week for two consecutive weeks was prescribed at 5mm from the surface of the vaginal cylinder in the IVBT arm. Patients in the ISBT arm were treated with 6Gy/fraction for four fractions over two days with Martinez Interstitial Perineal Template (MUPIT). Overall survival (OS) and disease-free survival (DFS) were the primary endpoints. Acute and late toxicities were the secondary endpoints.
Results
Median follow-up (FU) was 24.3 months and 32.8 months in the IVBT and ISBT arms respectively. The 3-year OS and DFS for IVBT and ISBT arms were 54% and 93% (p = 0.011) and 42% and 93% (p = 0.023), respectively. Both the arms had similar acute & late toxicities.
Conclusion
ISBT had significantly better survival outcomes in terms of OS, and DFS with similar toxicity as compared to IVBT in patients with residual or recurrent disease after inadvertent hysterectomy for cervical cancer.
Raman and autofluorescence spectroscopy for in situ identification of neoplastic tissue during surgical treatment of brain tumors
Authors: Uckermann Ortrud , Ziegler Jonathan , Meinhardt Matthias , Richter Sven , Schackert Gabriele , Eyüpoglu Ilker Y. , Hijazi Mido M. , Krex Dietmar , Juratli Tareq A. , Sobottka Stephan B. , Galli Roberta 
Published: August 2024

Abstract

Purpose
Raman spectroscopy (RS) is a promising method for brain tumor detection. Near-infrared autofluorescence (AF) acquired during RS provides additional information useful for tumor identification and was investigated in comparison with RS for delineating brain tumors in situ.
Methods
Raman spectra together with AF were acquired in situ within the solid tumor and at the tumor border during routine brain tumor surgeries (218 spectra; glioma WHO II-III n = 6, GBM n = 10, metastases n = 10, meningioma n = 3). Tissue classification for tumor identification in situ was trained on data acquired ex vivo (375 spectra; glioma/GBM patients n = 20, metastases n = 11, meningioma n = 13, epileptic hippocampi n = 4).
Results
Both in situ and ex vivo data showed that AF intensity in brain tumors is lower compared to border regions and normal brain tissue. Moreover, a positive correlation was observed between the AF intensity and the intensity of the Raman band of lipids at 1437 cm− 1, while a negative correlation was found with the intensity of the protein band at 1260 cm− 1. The classification of in situ AF and RS datasets matched the surgeon’s evaluation of tissue type with a correct rate of 0.83 and 0.84, respectively. Similar correct rates were achieved in comparison to histopathology of tissue biopsies resected in selected measurement positons (AF: 0.80, RS: 0.83).
Conclusions
Spectroscopy was successfully integrated into existing neurosurgical workflows and in situ spectroscopic data could be classified based on ex vivo data. RS confirmed its ability in detecting brain tumors, while AF emerged as a competitive method for intraoperative tumor delineation.
Epidemiological survey on pet dog ticks (Rhipicephalus turanicus sensu stricto) and their blood samples in Korla, northwestern China
Authors: Chen Jianhui , Shi Shen , Cui Nannan , Su Lixin , Wang Ziqi , Li Fang , Zhan Shang , Zhao Guoyu , Wang Yuanzhi , Wang Xijiang 
Published: August 2024

Abstract

Background
Pet dogs pose a potential risk to transmitting zoonotic pathogens by ticks. however information about the prevalence status in pet dog of Tick-borne diseases is currently limited.
Result
In the study, 196 blood samples and 223 parasitic ticks were collected from pet dogs in Korla, northwestern China. Based on morphological and molecular characteristics, all ticks were identified as Rhipicephalus turanicus sensu stricto.We used primers targeting the 16S ribosomal(16S rRNA) for detection of Anaplasma bovis species, targeting the small subunit 18S ribosomal RNA gene (18S rRNA) for detection of Hepatozoon canis species and targeting htpAB-associated repetitive element gene (IS111) for detection of Coxiella burnetii species The nPCR-positive products were sequenced, aligned, and phylogenetically analyzed. Three tick-borne pathogenic bacteria were detected in the samples. Coxiella burnetii were detected both in parasitic ticks and in blood samples with a detection rate of 17.93% (40/233) in ticks and 79.1% (155/196) in blood samples, followed by 21.52% Hepatozoon canis (48/233) in tick, 2.5% Anaplasma bovis (5/196) in blood samples.
Conclusion
This study provided molecular evidence for the occurrence of A. bovis, H. canis and C. burnetii circulating in pet dogs and their parasitic ticks in northwestern China. Understanding the prevalence of Tick-borne diseases in pet dog is essential for developing effective strategies for disease control and management.
Ileal Signet Ring Adenocarcinoma in Crohn’s Disease: Unanticipated Diagnosis After Surgical Resection of a Symptomatic Stricture
Authors: Clarke Lindsay M. , Riascos Maria Christina , Redston Mark S. , Hamilton Matthew J. , Kelly Colleen R. 
Published: August 2024

Abstract

We describe a rare case of small bowel signet ring adenocarcinoma that developed in the context of ileal Crohn’s disease. The patient had medically refractory, stricturing disease for several years, leading to obstructive symptoms necessitating resection, which ultimately led to the diagnosis. This case highlights the importance of considering malignancy in the differential and surgical referral earlier in the treatment paradigm for obstructive symptoms that do not respond to IBD-directed therapies. Since there currently are no screening guidelines for small bowel malignancy in CD, close clinical monitoring is recommended in an effort to reduce complications.
Risk factors of poor prognosis in patients with acute ischemic stroke after bridging therapy
Authors: wei wan , Qiu Kefan , Nie Tian , Feng Danyu , Liu Fei , Zhu Jiahui , Huang Chao , Hong Xiaoqin , Zhang Liuhai 
Published: August 2024

Abstract

Background: The poor prognosis of patients with acute ischemic stroke (AIS) after bridging therapy (BT) imposes a heavy burden on their families. This study decided to investigate the risk factors for poor prognosis and establish a predictive model.Objective: To explore the risk factors of poor prognosis in patients with AIS after BT.Methods: The study included AIS patients treated with BT (intravenous thrombolysis with alteplase prior to endovascular thrombectomy) from January 2020 to December 2023 in the Hangzhou First People's Hospital. Modified Rankin scale (mRS)was used to assess the patient’s prognosis after 3 months, and these patients were divided into the poor prognosis group (mRS > 2) and good prognosis group (mRS ≤ 2) according to the mRS.The patients' history of chronic diseases and the laboratory testing data were recorded. SPSS 25 was used for statistical analysis.Receiver operating characteristics (ROC) curves and logistic regression analysis were used to explore associated factors of AIS treated with BT.Results: We studied 120 AIS patients treated with BT.The poor prognosis group included 65 cases and good prognosis group included 55 cases.In the poor prognosis group, the patients with higher proportion of stroke-associated pneumonia (SAP), Symptomatic intracranial hemorrhage(sICH) and intracranial hemorrhage (ICH), and with higher NIHSS score at admission were older, concomitantly, the fasting plasma glucose (FBG) was significantly higher than those of the good prognosis group (P < 0.05). Multivariate logistic regression analysis showed SAP and NIHSS score were independent risk factors for poor prognosis of patients with AIS after BT (P < 0.05).The ROC analysis showed that the area under curve (AUC) of SAP was 0.717 (95% CI = 0.622–0.811), for the NIHSS score, the AUC was 0.716 (95% CI = 0.624–0.807), and the optimal cutoff threshold, sensitivity, and specificity were 15.4, 0.754, 0.564 respectively.When SAP combined with NIHSS score,we created a 2-item prediction model.In this model, the AUC increased to 0.809 (95% CI = 0.732–0.886), and the optimal cut-off, sensitivity, and specificity were 0.522,0.831, 0.691 respectively.Conclusion: Age, FBG, SAP, sICH ,ICH, and NIHSS score at admission were associated with poor prognosis of AIS patients after BT, while SAP and NIHSS score were independent risk factors for poor prognosis. The NIHSS score plus the SAP had a high diagnostic performance and predictive value for poor prognosis in patients with AIS treated with BT.
Epidemiological Characteristics of Imported Malaria Related to International Travel in the Republic of Korea (2009-2018)
Authors: Jeon Byoung Hak , Lee Jung Ah , Lee Shin Young , Lee Sang Eun , Yeom Joon Sup 
Published: August 2024

Abstract

Malaria, transmitted by mosquitoes infected with Plasmodium parasites, remains a significant health issue with global travel increasing the risk of imported malaria. This study investigates imported malaria cases in the Republic of Korea from 2009 to 2018 using data from the Korea National Infectious Disease Surveillance System. During this period, 601 imported cases were reported, with 82.4% male patients and a median age of 39.1 years. Most cases (76.5%) involved Korean residents returning from malaria-endemic areas, mainly Africa and Asia. Plasmodium falciparum (55.7%) and Plasmodium vivax (30.3%) were the predominant species. The annual percent change in incidence rate was 6.45%. Notably, 71.5% of the patients did not receive prophylactic chemotherapy, and 18% of those who did still developed malaria. Median diagnostic delays were 4 days for P. falciparum and 7 days for P. vivax. The case fatality rate was 2.3%, with all deaths occurring in residents who contracted P. falciparum in Africa. This study emphasizes the ongoing risk of imported malaria in the ROK and highlights the need for better awareness and preventive measures among travelers. Enhancing surveillance and educating travelers on prophylaxis are crucial.
Integrated single-cell and bulk RNA sequencing analyses reveal the impact of tryptophan metabolism on prognosis and immunotherapy in colon cancer
Authors: Hu Yanyan , Xu Ximo , Zhong Hao , Ding Chengshen , Qin Wei , Zhang Enkui , Shu Duohuo , Yu Mengqin , Abuduaini Naijipu , Yang Xiao , Feng Bo , Li Jianwen , zhang sen 
Published: August 2024

Abstract

Tryptophan metabolism is intricately associated with the progression of colon cancer. This research endeavored to meticulously analyze tryptophan metabolic characteristics in colon cancer and forecast immunotherapy responses. Patients were stratified into subtypes through consistent clustering, and a tryptophan metabolic risk score model was constructed using the random forest algorithm. Based on these risk scores, patients were delineated into high and low-risk groups, and their clinicopathologic characteristics, immune cell infiltration, immune checkpoint expression, and signaling pathway disparities were examined. The Oncopredict algorithm facilitated the identification of sensitive chemotherapeutic agents, while the immune escape score was employed to evaluate the immunotherapy response across risk groups. Transcriptomic sequencing findings were corroborated by single-cell sequencing from Shanghai Ruijin Hospital. Two distinct subtypes of colon cancer patients emerged, exhibiting significant prognostic and immune cell infiltration differences. The high-risk group demonstrated a poorer prognosis (p<0.001), advanced clinical stage (p<0.001), and elevated immunosuppressive cell expression (p<0.05). Additionally, three chemotherapeutic drugs showed efficacy in the high-risk cohort, which also displayed a heightened immune escape potential (p<0.05) and diminished response to immunotherapy. Single-cell sequencing validated the overexpression of tryptophan-related genes in epithelial cells. In conclusion, tryptophan metabolism significantly influences the colon cancer immune microenvironment, with high-risk patients experiencing adverse prognoses and potentially reduced efficacy of immunotherapy.
Is ultrasound-guided radiofrequency ablation reliable for unifocal T1aN0M0 papillary thyroid carcinoma in the isthmus: A retrospective cohort study
Authors: Zhou Gongli , Xu Dong , Zhang Beibei , Su Ruiqing , Xu Ke , Zhang Xuefeng , Li Feng , Zhao Wei , Cai Tingting 
Published: August 2024

Abstract

431 patients who underwent ultrasound-guided RFA for unifocal T1aN0M0 papillary thyroid carcinoma (PTC) were divided by location of the lesion into the PTCI group (52 females, 7 males, mean age 43.79 ± 12.04 years, range 22–74 years) and the PTCL group ((291 females, 81 males, mean age 43.42 ± 10.87 years, range 18–75 years) for comparative analysis. The efficacy of ultrasound-guided RFA was evaluated by volume reduction rate (VRR), complete disappearance rate (CDR), and disease progression, and the safety was evaluated by incidence of complications. The two groups exhibited a consistent trend of change, with the PTCI group performing slightly better in volume, VRR, and CDR at all follow-up time points expect 1 month. The mean initial volume of the PTCI group vs the PTCL group decreased significantly from 65.4 ± 69.79 vs 86.38 ± 87.09mm³ (range 10.92-427.58 vs 3.05-471.6mm³) to 0 vs 0 mm³ at a mean follow-up time of 31.12 ± 12.5 months (range 12–60 months); their VRR increased significantly from − 618.62 ± 655.61% vs -789.85 ± 1135.07%, at 1 month to 100% vs 100% at 48 months. No disease progression was found in the two groups. The PTCI group had no complications, whereas the PTCL group had 7 complications (1.88%). Ultrasound-guided RFA is reliable for unifocal T1aN0M0 PTCI in the isthmus.
Validation of IASLC 9th Edition TNM Classification for Lung Cancer: Focus on N Descriptor
Authors: Son Joung Woo , Lee Joonseok , Jeon Jae Hyun , Cho Sukki , Shih Beatrice Chia-Hui , Jung Woohyun , Kim Kwhanmien , Jheon Sanghoon 
Published: August 2024

Abstract

Background: We externally validated the proposed 9th edition of the TNM staging classification with our institution's prospectively collected data and compared it to the 8th edition for overall survival (OS) and freedom from recurrence (FFR).
Methods: A retrospective analysis was conducted of 3,711 cases of stage I-III non-small cell lung cancer that underwent surgical treatment from January 2004 to December 2020. Survival was compared using Kaplan-Meier curves and multivariable Cox regression analysis. The concordance index (C-index), Alkaike information criterion (AIC), and R2 were used to assess the discriminatory ability.
Results: In the 9th edition, the N2 category (n = 347) was subdivided into N2a (n = 252, 72.6%) and N2b (n = 95, 27.4%). The TNM stage changes were as follows: 1) IIB to IIA, 140 cases (24.9%); 2) IIIA to IIB, 51 cases (11.4%); 3) IIIB to IIIA, 57 cases (59.4%); 4) IIIA to IIIB, 55 cases (12.5%). The survival curves of the proposed 9th edition demonstrated similar patterns to those of the 8th edition, but with a greater discriminative ability for OS and FFR. Subdividing N2 into N2a and N2b refined prognosis prediction. The C-index, AIC, and R2 demonstrated improved values in the proposed 9th edition compared to the 8th edition.
Conclusions: The proposed 9th edition of the TNM staging classification for lung cancer showed favorable prognostic validity and better discrimination ability than the 8th edition.