bluuebunny/crossref_metadata_2025_split huggingface dataset:
Menopause Hormone Replacement Therapy and Lifestyle Factors affect Metabolism and Immune System in the Serum Proteome of Aging Individuals
Authors: Dierks Clemens , Mizrak Roza Sürme , Shomroni Orr , Farztdinov Vadim , Textoris-Taube Kathrin , Ludwig Daniela , Rainer Johannes , Mülleder Michael , Demuth Ilja , Ralser Markus 
Published: June 2024

AbstractAging is a fundamental risk factor for a wide array of diseases. The Berlin Aging Study II (BASE-II) is a cohort study designed to investigate the physical, mental, and social determinants of successful aging. We utilized high-throughput mass spectrometry to measure the proteomes of 1890 BASE-II participants, divided into two age groups: 27-37 years and 60-85 years. We employed multiple linear regression analyses to explore the effects of demographic factors such as age, sex, and BMI, along with hormonal treatments and lifestyle factors, on the serum proteome. We identify new associations and confirm previously described proteins linked to age, sex, BMI and hormonal contraceptive use (HCU). Notably, we observed that the abundance of nutrient transport proteins, particularly apolipoproteins, is linked to metabolic diseases in aged individuals, including metabolic syndrome and type 2 diabetes. Additionally, we identified specific alterations explained by lifestyle factors, such as smoking and alcohol consumption. We further report a significant proteome signature in female study participants corresponding to menopause hormone replacement therapy (MHT). We successfully classified these participants based on MHT status with an AUROC of 0.82 using two proteins, Complement Component 9 and Plasminogen, slightly outperforming estradiol (AUROC: 0.80), the active ingredient in most MHT preparations. Overall, our study underscores the impact of lifestyle and hormonal therapies on the serum proteome during aging, primarily affecting components of the immune system and metabolism.
Respiratory syncytial virus (RSV) prevention: perception and willingness of expectant parents in the Netherlands
Authors: Harteveld Lisette M. , van Leeuwen Lisanne M. , Euser Sjoerd M. , Smit Lucy , Vollebregt Karlijn C. , Bogaert Debby , van Houten Marlies A. 
Published: June 2024

ABSTRACTObjectivesTo investigate the perception and willingness of pregnant women and their partners to accept maternal vaccination or neonatal immunization against respiratory syncytial virus (RSV).DesignA cross-sectional survey studySettingPregnant women and their partners were recruited through healthcare professionals (midwives, gynaecologists, and Youth Health care), social media platforms (Instagram, LinkedIn, Facebook via institutions like the Spaarne Hospital), and the 9-Months Fair in the Netherlands.Main outcome measuresWillingness and motivation in decision-making for both maternal RSV vaccination or neonatal RSV immunization among pregnant women and their partners, including strategy preferences, and informational needs.ResultsIn total 1001 pregnant women (mean age: 31.1 years) and their partners (mean age: 33.2 years) completed the survey. On average, they were 24 weeks pregnant at the time, and 54.6% had no other children yet. The majority was Dutch-born (95.2% of women); with 68.3% of women having completed higher education and with overall strong pro-vaccination attitudes (93.9% of partners intended to vaccinate their expected newborn). The overall acceptability to vaccination and immunization was high, with 87% of respondents indicating they would (likely) accept both strategies. A positive attitude towards both methods was associated with previous experience with severity of RSV, intention to vaccinate the newborn and parental vaccination status during childhood and current pregnancy. When the choice was given, the majority of participants, in particular those with children and the intention to breastfeed, favoured maternal vaccination over passive immunization of infants (75.3% of the pregnant and 71.6% of the partners). A majority of the respondents cited optimal protection for the child and knowledge of RSV as important factors for accepting RSV prophylaxis.ConclusionsWhile most participants would accept both strategies for RSV protection of their infant, a majority, especially those with other children, favored maternal vaccination, due to concerns about infant safety and awareness of RSV severity.
A Mendelian randomization study identifies the causal association between plasma mitochondrial CHCHD proteins and polycystic ovary syndrome
Authors: Wei Shiyang , Wang Yafeng , Liu Niping , Zhao Renfeng 
Published: June 2024

AbstractPurposeThe objective of this research was to examine the causal link between PCOS and plasma mitochondrial coiled-coil-helix-coiled-coil-helix domain(CHCHD) proteins using a Mendelian randomization (MR) method.MethodsWe performed a two-sample MR analyses by utilizing summary statistics obtained from genome-wide association studies (GWAS) of PCOS (642 cases and 118,228 controls) and the levels of CHCHD2 and CHCHD10 in plasma (3,301 individuals). The inverse-variance weighted (IVW) method was used for the MR analyses, along with additional sensitivity analyses.ResultsThe association between CHCHD2 and an increased risk of PCOS was identified (OR = 1.682; 95% CI = (1.231, 2.297); P = 0.001). The discovery of CHCHD10 revealed a protective impact on the likelihood of PCOS (OR = 0.828, 95% CI= 0.698-0.981, p = 0.029). The MR results were confirmed to be robust through the analysis of heterogeneity (P > 0.05) and pleiotropy (P > 0.05).ConclusionOur findings indicates that mitochondrial proteins CHCHD2 and CHCHD10 may play an important role in the pathogenesis of PCOS. Additional research is necessary to clarify the underlying mechanisms and investigate the potential of these proteins as targets for therapeutic intervention in PCOS.What does this study add to the clinical workA strong causal relationship has been established between two plasma mitochondrial complexes with coiled-coil-helix domains and polycystic ovary syndrome. The exact role of serum mitochondrial protein in polycystic ovary syndrome needs to be investigated via large-scale randomization trials or further studies.
Marseilleviruses biological properties and viral translation-associated proteins based onin silicotertiary structure
Authors: Yucheng XIA , Wang Long , Xu Yutao , Wong Yue-Him , Zhou Zhichao , Rui ZHANG 
Published: June 2024

AbstractMarseilleviruses are a group of double-strand DNA viruses that infect amoeba within theNucleocytoviricotaphylum and are ubiquitous in water and soil globally. Here, we report four novel strains isolated from mangroves in Guangdong province, China, namely, futianmevirus, futianmfvirus, dashavirus str. E, and xiwanvirus. Viral particles presented about 220∼240 nm icosahedrally shaped capsids and were wrapped by membranes to form giant vesicles. Based on stability assays, viral particles were halotolerant and acid-tolerant, but sensitive to chloroform and high temperature, while giant vesicles conferred thermal and acid/alkaline resistance to particles. Genomics and phylogenetic analyses showed that the four strains formed divergent branches within different lineages of marseillevirus. Notably, to our knowledge, futianmevirus was the first reported marseillevirus lacking translation elongation factor EF-1alpha (EF1A). Ourin silicoanalysis of marseillevirus coded translation-associated homolgs suggested their conserved functions. Additionally, we predicted at least four novel proteins that were structurally similar to components of the protozoan ribosome. Overall, not only our data comprehensively described the diversity of marseillevirus biological properties, but also proposed a new perspective on the giant virus translation system.ImportanceThe familyMarseilleviridaewas the second reported family of giant viruses and distributed globally. In this work, we reported the four novel marseilleviruses isolated from saltwater samples of mangrove. Difference of biological properties between giant vesicles and viral particles revealed the environment fitness of marseillevirus.On the other hand, sensitivity to chloroform indicated the importance of lipid components for viral infection. Additionally, our comparative genomics, phylogenetic analysis, and protein structure comparison revealed the diverse translation-associated gene sets of marseilleviruses. The prediction of ribosome components expands the knowledge about the giant viral translation-associated proteins, and will be helpful in future to reveal how giant viruses hijack the amoeba translation system.
A single mutation in dairy cow-associated H5N1 viruses increases receptor binding breadth
Authors: Good Marina R. , Ji Wei , Fernández-Quintero Monica L. , Ward Andrew B. , Guthmiller Jenna J. 
Published: June 2024

ABSTRACTClade 2.3.4.4b H5N1 is causing an unprecedented outbreak in dairy cows in the United States. To understand if recent H5N1 viruses are changing their receptor use, we screened recombinant hemagglutinin (HA) from historical and recent 2.3.4.4b H5N1 viruses for binding to distinct glycans bearing terminal sialic acids. We found that H5 from A/Texas/37/2024, an isolate from the dairy cow outbreak, has increased binding breadth to glycans bearing terminal α2,3 sialic acids, the avian receptor, compared to historical and recent 2.3.4.4b H5N1 viruses. We did not observe any binding to α2,6 sialic acids, the receptor used by human seasonal influenza viruses. We identified a single mutation outside of the receptor binding site, T199I, was responsible for increased binding breadth, as it increased receptor binding site flexibility. Together, these data show recent H5N1 viruses are evolving increased receptor binding breadth which could impact the host range and cell types infected with H5N1.
A Metagenomic Analysis of the Fecal Microbiota of the Critically Endangered Eastern Bongo
Authors: Kristopeit Suzanne M. , Murphy Kathryn A. , Bevis Durward L. , Brandt Janelle , McLaughlin Richard William 
Published: June 2024

AbstractThe Eastern Bongo (Tragelaphus eurycerus isaaci) is a critically endangered mammal. In zoos, this animal has been known to have a sensitive gastrointestinal tract. Using a metagenomic approach the purpose of this study was to determine the microbiota of two adult (n=2) Eastern Bongos living in a zoo in South Dakota, as well as a juvenile (n=6) over a six-week period to see the microbial succession, and to learn if there were pathogenic microorganisms present which are capable of causing gastrointestinal disease. The dominant phylum in seven samples was Bacillota with Pseudomonadota dominant in only one of the juvenile samples. Functional classifications showed Protein Synthesis was the most predominant (11.36–35.71%). Almost equally predominant were Energy and Precursor Metabolites Generation (7.00-13.27%) and Stress Response, Defense and Virulence (8.44-12.90%). Finally, we also determined the resistomes which will contribute to the One Health approach.
The Radical Pair Mechanism Cannot Explain Telecommunication Frequency Effects on Reactive Oxygen Species
Authors: Talbi Owaiss , Zadeh-Haghighi Hadi , Simon Christoph 
Published: June 2024

ABSTRACTIn order to investigate whether the radical pair mechanism (RPM) can explain the effects of telecommunication frequency radiation on reactive oxygen species production, we modelled the effects of oscillating magnetic fields on radical pair systems. Our analysis indicates that the RPM cannot account for the biological effects observed under exposure to telecommunication frequencies due to negligible effects under low-amplitude conditions used in experimental setups. Observable effects on radical pairs at these frequencies would require hyperfine coupling constants that are precisely fine-tuned to large values that far exceed those naturally occurring within biological systems. We conclude that some other mechanism must be responsible for the effects of telecommunication frequency fields in biological systems.
Hypertension incidence among individuals successfully treated for pulmonary tuberculosis
Authors: Ontiveros Victoria C , Kipiani Maia , Avaliani Teona , Auld Sara C , Gujabidze Mariam , Kempker Russell R , Kornfeld Hardy , Bryan Cassandra , Huaman Moises A , Magee Matthew J , Salindri Argita D 
Published: June 2024

ABSTRACTWe reported a cumulative incidence of hypertension of 28% among individuals successfully treated for pulmonary tuberculosis (TB) with no prior hypertension indication at TB treatment initiation (i.e., prevalent hypertension). The cumulative incidence of hypertension among those with ≥1 cavitary lesion was twice the cumulative incidence among those without cavitary lesions.
Combining chromatography coupled SAXS and AI-predicted structures to dissect the mechanism of ParB1-parS1partition assembly formation
Authors: Sodhi Anu , Lata Sneh , Chaudhuri Barnali N. 
Published: June 2024

ABSTRACTCoupling of solution SAXS and AI-predicted structures can be a powerful strategy for delineating subtle conformational changes and self-association in protein switches. ParB, which is a condensate-forming DNA clamp that aids in bacterial chromosomal origin segregation, undergoes CTP-induced conformational switching to enable DNA sliding. The nature ofparSDNA-induced conformational change in full-length ParB, and the structural features that govern self-association of ParB for partition assembly condensate formation, remains sparsely understood. We combined chromatography-coupled SAXS, rigid domains obtained from Alphafold model of ParB1 fromVibrio cholerae, and synthetic SAXS data describing known domain interfaces, to build integrative models of conformational states of full-length ParB1. These integrative models revealed howparS1DNA loading primed ParB1 for clamping and sliding. The CTPase domains in ParB1 were moved nearer upon DNA loading to facilitate clamping, and a lumen lined with a weak DNA binding site was formed belowparS1binding site for capturing the sliding DNA. Furthermore, we showed that an N-terminal segment of ParB1 undergoes concentration-dependent oligomerization. An intrinsically disordered linker joining this oligomerization-prone N-terminal segment and the C-terminal domain curbs self-association of full-length ParB1, which is likely relevant for ParB1-mediated higher order partition assembly formation. To summarize, SAXS and Alphafold were effectively combined to provide unique insights into context-specific domain rearrangements and self-association in ParB1 for the mechanistic understanding of partition assembly formation.
Synthesis, spectroscopic and crystallographic characterization of various cymantrenyl thioethers [Mn{C5H
x
Br
y
(SMe)
z
}(PPh3)(CO)2]
Authors: Klein-Hessling Christian , Blockhaus Tobias , Sünkel Karlheinz 
Published: June 2024

Starting from [Mn(C5H4Br)(PPh3)(CO)2] (1a), the cymantrenyl thioethers [Mn(C5H4SMe)(PPh3)(CO)2] (1b) and [Mn{C5H4–n
Br(SMe)
n
}(PPh3)(CO)2] (n = 1 for compound 2, n = 2 for 3 and n = 3 for 4) were obtained, using either n-butyllithium (n-BuLi), lithium diisopropylamide (LDA) or lithium tetramethylpiperidide (LiTMP) as base, followed by electrophilic quenching with MeSSMe. Stepwise consecutive reaction of [Mn(C5Br5)(PPh3)(CO)2] with n-BuLi and MeSSMe led finally to [Mn{C5(SMe)5}(PPh3)(CO)2] (11), only the fifth complex to be reported containing a perthiolated cyclopentadienyl ring. The molecular and crystal structures of 1b, 3, 4 and 11 were determined and were studied for the occurrence of S...S and S...Br interactions. It turned out that although some interactions of this type occurred, they were of minor importance for the arrangement of the molecules in the crystal.
Between branding and being: how are inclusive city branding and inclusive city practices related?
Authors: Zhao Run , Edelenbos Jurian , de Jong Martin 
Published: June 2024

Purpose
The purpose of this study is to investigate the relationship between being an inclusive city and branding oneself as such, as more cities adopt the inclusive city concept as part of their brand identity.

Design/methodology/approach
This paper builds theory by introducing a typology that categorizes cities based on their level of inclusion and degree of branding, supplemented by an analysis of the branding practices and identities. Integrating the literature on inclusive city and city branding, with a specific focus on the inherent conflict between their sharing and competing attributes, this research postulates that a city may choose to engage in being inclusive and branding itself as such in various ways depending on its dominant motivations of altruism or entrepreneurialism.

Findings
Four distinct types of inclusive city branding are identified: inclusion ambassadors (high inclusion and high branding); innate champions (high inclusion and low branding); façade marketers (low inclusion and high branding); and silent segregators (low inclusion and low branding). Furthermore, it underscores that inclusive city branding is shaped by the interplay of entrepreneurialism and altruism, not just a city’s inclusion. Different branding practices, such as media-generated images, narratives and events, are emphasized when entrepreneurialism is the primary motivation, whereas iconic architecture buildings, flagship projects and long-term policies are more associated with altruism.

Originality/value
This study develops a typology to unravel the paradoxical aspects of inclusive city branding. Examining the intersection of city branding motivations and practices enriches existing literature. Moreover, its findings offer valuable insights for cities grappling with the implementation of contentious inclusive branding strategies, thereby bridging theory with practical applications.
Exploring the structural relationships between augmented reality quality, perceived value, and psychological well-being in museum applications: a PLS-SEM analysis
Authors: Ma Dongming , Cheng Anping , Qian Hao 
Published: June 2024

PurposeThe purpose of the study is to examine the impact of augmented reality (AR) on users' tour experience and psychological well-being in museums. The study utilizes the Information System (IS) Success Model and the Perceived Value Theory to investigate the relationships between AR quality, content quality, system quality, vividness, utilitarian value, hedonic value, tour experience, and psychological well-being.Design/methodology/approachThe study adopts a quantitative research approach and surveys 286 visitors at the Wuhan Natural History Museum. The collected data is analyzed using PLS-SEM to test the proposed model.FindingsThe study finds that content quality, system quality, and vividness significantly influence utilitarian and hedonic value. Utilitarian and hedonic values mediate the relationship between AR quality and tour experience and psychological well-being, with hedonic value having a greater impact. Utilitarian value positively influences customers' hedonic value, while tour experience positively influences customers' psychological well-being.Originality/valueThis study contributes to the existing literature by applying the Information System Success Model and Perceived Value Theory to explore the impact of AR on users' tour experience and psychological well-being in museums. It provides empirical evidence supporting the importance of content quality and perceived value in promoting AR adoption and use. The findings add value to the field of augmented reality research and offer practical implications for AR service providers.
Utilization of agro waste activated carbons as carbon black replacement in electron beam irradiated styrene butadiene rubber composites
Authors: Radi H , El-Nemr Khaled F. , Elmesallamy Salwa M. , Amdeha Enas 
Published: June 2024

Purpose
This study aims to prepare activated carbon (AC) and activated biochar (BC) from sugarcane bagasse (SCB) can be used as carbon black (CB) replacement for styrene butadiene rubber (SBR) composites cured by electron beam (EB) radiation.

Design/methodology/approach
This study is carried out to investigate the effect of partial replacement of CB (as traditional filler) by AC or BC prepared from low-cost agricultural wastes (SCB) to improve the properties of SBR rubber cured by EB radiation (doses from 25 to 150 kGy).

Findings
The results indicated that the addition of AC or BC leads to improve the physical and mechanical properties of SBR with increasing irradiation dose [especially at concentration of 10 parts per hundred part of rubber (phr) from BC]. Also in this study, this paper examines how exposure of SBR rubber composites to ultraviolet (UV) radiation changes the mechanical properties for these composites, to do that, the specimens were examined before and after they were exposed to UV radiation for 300 h. The results showed that, the irradiated SBR composites, UV exposure, exhibit better retention in mechanical properties as compared with unirradiated ones, and the samples loaded with CB hybrid with ACs had an increased value of tensile strength (TS) retention as compared with blank sample.

Originality/value
The importance of this study is that, the production of AC from SCB offers a huge opportunity to overcome the problem of the disposal of SCB.
Are metaverse applications in quality 4.0 enablers of manufacturing resiliency? An exploratory review under disruption impressions and future research
Authors: El Jaouhari Asmae , Arif Jabir , Samadhiya Ashutosh , Kumar Anil , Jain Vranda , Agrawal Rohit 
Published: June 2024

PurposeThe purpose of this paper is to investigate, from a thorough review of the literature, the role of metaverse-based quality 4.0 (MV-based Q4.0) in achieving manufacturing resilience (MFGRES). Based on a categorization of MV-based Q4.0 enabler technologies and MFGRES antecedents, the paper provides a conceptual framework depicting the relationship between both areas while exploring existing knowledge in current literature.Design/methodology/approachThe paper is structured as a comprehensive systematic literature review (SLR) at the intersection of MV-based Q4.0 and MFGRES fields. From the Scopus database up to 2023, a final sample of 182 papers is selected based on the inclusion/exclusion criteria that shape the knowledge base of the research.FindingsIn light of the classification of reviewed papers, the findings show that artificial intelligence is especially well-suited to enhancing MFGRES. Transparency and flexibility are the resilience enablers that gain most from the implementation of MV-based Q4.0. Through analysis and synthesis of the literature, the study reveals the lack of an integrated approach combining both MV-based Q4.0 and MFGRES. This is particularly clear during disruptions.Practical implicationsThis study has a significant impact on managers and businesses. It also advances knowledge of the importance of MV-based Q4.0 in achieving MFGRES and gaining its full rewards.Originality/valueThis paper makes significant recommendations for academics, particularly those who are interested in the metaverse concept within MFGRES. The study also helps managers by illuminating a key area to concentrate on for the improvement of MFGRES within their organizations. In light of this, future research directions are suggested.