bluuebunny/crossref_metadata_2025_split huggingface dataset:
Iodine-123-Iomazenil Single-Photon Emission Computed Tomography Revealed Recovery of Neuronal Viability in Association With Improvement of Cognitive Dysfunction After Revascularization in Moyamoya Disease
Authors: Yoshioka Hideyuki , Wakai Takuma , Hashimoto Koji , Tateoka Toru , Fukuda Norito , Horiuchi Ryo , Umeda Takako , Onishi Hiroshi , Kinouchi Hiroyuki 
Published: July 2024

BACKGROUND AND OBJECTIVES:
Recent studies indicate that 123I-iomazenil (123I-IMZ) single-photon emission computed tomography (SPECT) can demonstrate neuronal viability. Although cognitive dysfunction has been recognized as an important issue in adult patients with moyamoya disease (MMD), no standard neuroradiological methods to define such conditions have been established. We examined the relationship between cognitive function and 123I-IMZ SPECT before and after revascularization in patients with MMD.

METHODS:
The study participants were 16 adult patients with MMD whose cerebrovascular reactivities were decreased only on the surgical sides of combined revascularization. Cognitive function was examined using the Mini-Mental State Examination (MMSE; cutoff: 27) and the Frontal Assessment Battery (FAB; cutoff: 16) before and at 3 to 6 months after surgery. 123I-iodoamphetamine (123I-IMP) SPECT with acetazolamide challenge and 123I-IMZ SPECT were performed concurrently while evaluating cognitive function. The radioreactivities of 123I-IMZ SPECT in regions with decreased cerebrovascular reactivities on 123I-IMP SPECT were investigated using affected-to-contralateral side asymmetry ratio (IMZ-ACR).

RESULTS:
Twelve patients showed normal cognitive function (MMSE: 29.8 ± 0.4, FAB: 18 ± 0) before surgery. No evident laterality of 123I-IMZ uptake was seen (IMZ-ACR: 0.98 ± 0.04). Neither cognitive function nor 123I-IMZ SPECT worsened after surgery (MMSE: 29.8 ± 0.3, FAB: 18 ± 0, IMZ-ACR: 1.00 ± 0.04). By contrast, 4 patients presented cognitive dysfunction (MMSE: 24.3 ± 3.9, FAB: 14.8 ± 2.7) before revascularization. Preoperative imaging of these patients showed decreased 123I-IMZ uptake, and their IMZ-ACRs (0.83 ± 0.08) were significantly lower than those of the normal group. After revascularization, cognitive functions and 123I-IMZ uptake tended to ameliorate (MMSE: 27.5 ± 1.7, FAB: 16.3 ± 2.2, IMZ-ACR: 0.94 ± 0.09).

CONCLUSION:
Preoperative cognitive function was associated with 123I-IMZ uptake in adult patients with MMD. After revascularization, cognitive function could be recovered in the viable areas of the brain, which is consistent with 123I-IMZ SPECT findings.
Improved whole-mount immunofluorescence protocol for consistent and robust labeling of adult Drosophila melanogaster adipose tissue
Authors: Ott Rachael K. , Williams Isaiah H. , Armstrong Alissa R. 
Published: July 2024

ABSTRACT
Energy storage and endocrine functions of the Drosophila fat body make it an excellent model for elucidating mechanisms that underlie physiological and pathophysiological organismal metabolism. Combined with Drosophila's robust genetic and immunofluorescence microscopy toolkits, studies of Drosophila fat body function are ripe for cell biological analysis. Unlike the larval fat body, which is easily removed as a single, cohesive sheet of tissue, isolating intact adult fat body proves to be more challenging, thus hindering consistent immunofluorescence labeling even within a single piece of adipose tissue. Here, we describe an improved approach to handling Drosophila abdomens that ensures full access of the adult fat body to solutions generally used in immunofluorescence labeling protocols. In addition, we assess the quality of fluorescence reporter expression and antibody immunoreactivity in response to variations in fixative type, fixation incubation time, and detergent used for cellular permeabilization. Overall, we provide several recommendations for steps in a whole-mount staining protocol that results in consistent and robust immunofluorescence labeling of the adult Drosophila fat body.
Influence of diet on the course and activity of Crohn's disease and ulcerative colitis - a review of available literature
Authors: Dębińska Julia , Bentkowska Zuzanna , Golemo Jagna , Szpyra Justyna , Kałuża Izabela , Górska Magdalena , Dziuba Gabriela , Bogoń Aleksandra , Ostojska Magdalena , Miazga Małgorzata 
Published: July 2024

Introduction: Inflammatory bowel disease (IBD) is a chronic inflammatory disease of the digestive tract. It is a clinical entity that includes: Crohn's disease (CD) and ulcerative colitis (UC). Food intake is an important environmental factor that influences the development of IBD. This article reviews studies on different dietary options and their effects on the course and activity of Crohn's disease and ulcerative colitis.
Materials and methods: A review of the literature available in the PubMed database was carried out, using the key words: ”inflammatory bowel disease”; ”Crohn disease”; ”diet”; “nutrition”; „colitis ulcerosa”.
Results: Appropriate diet selection is important in the course of inflammation in the intestines. It influences disease activity. It turns out that the diet improves inflammation parameters, clinical symptoms, and quality of life. In patients with inflammatory bowel disease, one should also remember about deficiencies such as iron, vitamin D or protein deficiency, the demand of which is increased in sick patients compared to the general population.
Conclusions: An individualized approach to nutritional therapy may be one of the main branches of treatment for patients with inflammatory bowel diseases. However, there is a need for further research on larger groups of patients in order to effectively implement it into treatment standards.
FIXING THE PERIPROSTHETIC FEMUR FRACTURE PROBLEM: ARE WE ABLE TO PROVIDE SAFE, EFFECTIVE, AND TIMELY CARE IN NORTH WALES? A PAN BETSI COLLABORATIVE STUDY
Authors: Kandhari V. , Shetty S. , Nugur A. , Ghosh S. , Azam A. , Bhaskar D. , Malek I. 
Published: July 2024

The recruitment drive, investment and collaboration within Betsi Cadwaladr University Health Board (BCUHB) sites providing specialist lower limb arthroplasty and trauma service has evolved over last few years with aims to improve patient care and reduce reliance on tertiary referral centres. Through our service-evaluation project, we reviewed the results of treatment provided for periprosthetic femur fractures (PPFFs) presenting to BCUHB sites over last 4 years.We retrospectively reviewed consecutive PPFFs admitted at three BCUHB sites from January’20 to June’23 with mean follow-up of 20.8 ± 13.2 (8–49) months [n=161; Mean age: 82.2 ± 8.5 (59–101) years, Females:107]. Over the review period we noted a 23% increase in service demand for care of PPFFs. Majority were managed surgically [132/161] [38 revision arthroplasties; 94 ORIFs] at BCUHB sites and two patients were referred to tertiary centre. Average time to surgery was 3.5 days. 90% of the PPFFs were managed successfully with 10% (16/159) having orthopaedic complications needing further intervention. 6.3 (10/159) had medical complications and did not need orthopaedic re-intervention. In our series, 12-month re-operation rate was 6.1% (8/132) and 1-month, 3-month and 1-year mortality rate was 6.3%, 11.3% and 21.4% respectively. These results are comparable to the published results of PPFF management at tertiary centres. Potential cost savings compared to transfer to tertiary centre for PPFF management was £2.31 million. Thus, it is possible to successfully provide adequate care for PPFFs at DGHs and efforts should be made to appropriately equip and adequately staff DGHs, to provide service for local PPFF care.
PATELLAR HEIGHT IN CHILDREN: ARE THE INDICES USED IN ADULTS APPROPRIATE IN THE PAEDIATRIC POPULATION?
Authors: Lal A. K. , Nugur A. , Santhanam S. 
Published: July 2024

Abnormal patella height has been found to be one of the main reasons for abnormal contact between patella and trochlear groove leading to patellar instability in children. Many methods have been described to diagnose patellar instability but most of them are justified only in adults. The reason being incomplete ossification in the paediatric population. These methods have been divided into direct and indirect methods. We analysed the MRI scan of knee of 57 children between 12–14 years of age with no previous diagnosis of patellar instability. Patients with a diagnosis of patellar instability, previous surgery on the knee or trauma and poor MRI scan were excluded from the study. We used Insall -Salvati Index (ISI), Caton-Deschamp Index (CDI) and Patella-Trochlear Index (PTI) and compared the results. We found that 40% of measurements by CDI and 41% by ISI showed patella alta in patients with normal patella height. 10% of patients in PTI readings had value suggestive of abnormal patella height.We concluded that PTI is a more reliable index to be used in children as it uses the length of articular surface and does not rely on bony landmarks. Studies done show PTI is a more reliable and accurate method of measuring patella height.
Improved echo signal detection for pseudo-random single-photon counting laser ranging
Authors: Qu Shihan , Li Guobing , Li Jie , Zhang Guomei 
Published: July 2024

In this paper, a new echo signal detection method, to the best of our knowledge, for pseudo-random single-photon counting ranging (PSPCR) LiDAR systems is proposed, which is applied for long distances, low repetition rates, and system cost reduction. First, in order to achieve a comparable temporal resolution as that in time-correlated single-photon counting (TCSPC) systems, we extend the pseudo-random code to discriminate the minimal time slot in time correlation. Second, we use the full width at half maxima (FWHM) in the duration of each pseudo-random code for correlation to reduce the impact of pulse width variation and timing jitters on ranging accuracy. Third, we study the bias and errors caused by using synchronous signals as the “START” signal, and propose to use the time of flight (ToF) at half energy to reduce the walk error. Simulation results show that, compared with existing PSPCR methods, the proposed method improves ranging accuracy with a lower repetition rate and lower peak and average power—centimeter-level ranging accuracy over tens of kilometers can be achieved using a laser with a repetition rate of 400 kHz, peak power of up to 1 kW, and average power of up to 1 W.
Optically powered FiWi system for B5G hybrid VLC/RF access networks
Authors: Pinto Felipe B. F. , Souza Leticia C. , Andrade Tomás P. V. , Lima Eduardo S. , Anderson Evandro L. , Portelinha Jr. Francisco M. , Cerqueira S. Jr. Arismar 
Published: July 2024

This paper proposes the concept and reports the demonstrator of an optically powered fiber/wireless (FiWi) system based on a hybrid visible light communication (VLC) and RF access network for beyond 5G (B5G) indoor applications. The power-over-fiber (PoF) technology is properly applied to simultaneously energize two components from the remote site, namely: a radio-over-fiber (RoF) module, which contains a photodetector and an RF amplifier; a red laser from the VLC system. The proposed PoF system is composed of a 500 m conventional multimode optical fiber (MMF) with a 62.5 μm core diameter, which enables the transmission of up to 12 W optical power with a power transmission efficiency (PTE) of 14%. In the proof of concept, 8.1 W optical power is generated to feed the 5G system components. Regarding the communication setup, a 5G new radio (5G NR) signal is initially transported through a RoF fronthaul, composed of 10 km of single-mode fiber (SMF), followed by the hybrid VLC/RF access network. The performance investigation of the B5G communication system is based on an analysis of the root mean square error vector magnitude (EVMRMS) parameter. Experimental results indicate 1.2 Gbps throughput in the VLC link using a 200 MHz bandwidth and 64-quadrature amplitude modulation (64-QAM), resulting in approximately 4.02% of EVMRMS. Moreover, the RF link at 3.5 GHz provides 360 Mbps with an EVMRMS of 2.67%. Finally, a comparison between the PoF system and conventional power supply validates the proposed approach's applicability for B5G wireless communications.
Broadband continuous-wave mid-infrared wavelength conversion in high-Q silicon microring resonators
Authors: Yan Zhiwei , Yi Qiyuan , Li Qiyuan , Cheng Guanglian , Cui Shuai , Xia Lipeng , Xu Fanglu , Xiong Xinzhe , Shen Zengfan , Zheng Shuang , Yu Yuan , Zou Yi , Shen Li 
Published: July 2024

The four-wave mixing (FWM) effect offers promise to generate or amplify light at wavelengths where achieving substantial gain is challenging, particularly within the mid-infrared (MIR) spectral range. Here, based on the commonly used 340 nm silicon-on-insulator (SOI) platform, we experimentally demonstrate high-efficiency and broadband wavelength conversion using the FWM effect in a high-Q silicon microring resonator pumped by a continuous-wave (CW) laser in the 2 μm waveband. The microring resonator parameters are carefully optimized for effective phase-matching to obtain high conversion efficiency (CE) with broad bandwidth. The loaded quality (Q
l
) factor of the fabricated microring resonator is measured to be 1.11×105, at a resonance wavelength of 1999.3 nm, indicating low propagation losses of 1.68 dB/cm. A maximum CE of −15.57  dB is achieved with a low input pump power of only 4.42 dBm, representing, to our knowledge, the highest on-chip CE demonstrated to date under the CW pump in the MIR range. Furthermore, broadband wavelength conversion can be observed across a 140.4 nm wavelength range with a CE of −19.32  dB, and simulations indicate that the conversion bandwidth is over 400 nm. This work opens great potential in exploiting widely tunable on-chip sources using high-efficiency wavelength conversion, particularly leveraging the advantages of the SOI platform in integrated photonics across the 2 μm MIR range.
Six-year follow-up of participants in two clinical trials of rituximab or cyclophosphamide in Myalgic Encephalomyelitis/Chronic Fatigue Syndrome
Authors: Rekeland Ingrid G. , Sørland Kari , Neteland Lisbeth Lykke , Fosså Alexander , Alme Kine , Risa Kristin , Dahl Olav , Tronstad Karl J. , Mella Olav , Fluge Øystein 
Published: July 2024

Objectives
In this six-year follow-up study, we used patient-reported outcome measures (PROMs) to compare values at baseline, at 18 months, and at six-year follow up from the CycloME and the RituxME trials.

Methods
Based on the hypothesis that ME/CFS in a subgroup of patients is a variant of an autoimmune disease, we performed two clinical trials between 2014 and 2017. The RituxME trial was a randomized, double-blind and placebo-controlled phase III trial of 151 patients, assessing the B-cell depleting antibody rituximab. The CycloME trial was an open-label phase II trial of 40 patients using intravenous cyclophosphamide. Here we report six-year follow-up from both trials, using the Short Form 36 Physical Function (SF-36 PF) and DePaul short form (DSQ-SF) questionnaires.

Result
Of the patients available after six years, 75.7% of RituxME and 94.4% of CycloME patients participated. In the RituxME rituximab group, the mean SF-36 PF scores were 32.9 at baseline, 42.4 at 18 months and 45.5 at six years. In the placebo group, the mean SF-36 PF scores were 32.3 at baseline, 45.5 at 18 months and 43.1 at six years. In the CycloME trial, mean SF-36 PF increased from 35.4 at baseline to 54.4 at 18 months, and 56.7 at six years. At six-year follow-up, 44.1% of cyclophosphamide-, 27.6% of rituximab- and 20.4% of placebo-treated patients had an SF-36 PF ≥ 70, and further, 17.6%, 8.6% and 7.4% of the corresponding patient groups had an SF-36 PF ≥ 90, which is within normal range. In terms of worsening at six years, 5.9% of cyclophosphamide-treated, 10.3% of rituximab-, and 14.8% of placebo-treated patients had a drop in SF-36 PF of 20 points or more from baseline. There were no serious unexpected adverse reactions.

Conclusions
After six years, 44.1% of the cyclophosphamide group scored an SF-36 PF of at least 70, and 17.6% of at least 90, suggesting that cyclophosphamide in a subgroup may modulate the disease course in a beneficial way. However, cyclophosphamide carries toxicity concerns and should not be used for ME/CFS patients outside clinical trials. Rather, these data should encourage efforts to better understand the disease mechanisms and to search for targeted and less toxic immune modulatory treatment for this patient group.
HTA model for laboratory medicine technologies: overview of approaches adopted in some international agencies
Authors: d’Angela Daniela , Orso Massimiliano , Migliore Antonio , Polistena Barbara , Spandonaro Federico , Bernardini Sergio 
Published: July 2024

Abstract
The Health Technology Assessment (HTA) Working Group of the Emerging Technology Division of International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) aims to develop a methodological approach for producing structured HTA information for laboratory medicine technologies. This approach seeks to support decision-making processes at the country, regional, and/or hospital levels regarding the introduction of specific technologies. The focus of this model will primarily be on defining assessment elements within the domains of ‘organizational aspects’ and ‘costs and economic evaluations’, potentially differentiated by the type of diagnostic technology (e.g., genetic tests, molecular tests). To achieve this project’s goal, a literature review and examination of websites of international HTA agencies have been conducted. The research aims to identify multidisciplinary methodological approaches used to assess laboratory diagnostic technologies and to pinpoint the domains and assessment elements utilized. We found 7 methodological articles describing methodological approaches adopted to assess laboratory diagnostic technologies. Among the HTA organizations considered, 23 reports were found, of which 7 were produced by the European Network of HTA (EUnetHTA), 4 by the National Institute for Health and Care Excellence Diagnostic Assessment Program (NICE DAP), and 12 by other HTA agencies. The EUnetHTA reports were rapid collaborative assessments covering various domains, while the NICE DAP reports focused on diagnostic guidances, including descriptions of technologies, clinical need and practice, diagnostic tests, accuracy, effectiveness, and cost-effectiveness. Finally, a survey targeting laboratory professionals will be conducted to introduce assessment elements, differentiated by the type of diagnostic technology, primarily for organizational and economic domains.
The evaluation of melatonin and EGF interaction on breast cancer metastasis
Authors: Akbarzadeh Moloud , Vahedian Vahid , Hamid Abudulmohesen Zahraa , Ghadimi Parvin , Fathi Maroufi Nazila , Farzaneh Ali , Bastani Sepideh , Roshanravan Neda , Pirpour Tazehkand Abbas , Fattahi Amir , Faridvand Yousef , Talebi Mehdi , Farajzadeh Davoud , Akbarzadeh Maryam 
Published: July 2024

Abstract

Objectives
Metastasis in breast cancer is the first cause of death in patients. The epidermal growth factor (EGF) increases cancer cells’ invasion, and migration. Melatonin’s inhibitory effects on various types of cancer were confirmed. This study aimed to investigate whether melatonin could apply its impact through the EGF-related pathways or not.

Methods
First, MDA-MB-231 and MCF7 cells were cultured, and then melatonin effects on cell viability were determined by MTT assay. Transwell invasion assay was applied to identify the invasiveness of these breast cancer cell lines under treatment of EGF and melatonin. Real-time RT-PCR then investigated the expression of MMP9 and MMP2 in determined groups. Cell proliferation was also assayed under EGF and melatonin treatment using Ki67 assessment by flow cytometry.

Results
The rate of invasion and migration of EGF-treated cells increased in both groups, in which melatonin caused increased invasion by EGF just in MCF7 cells. MMP9 and MMP2 expression increased significantly in both cell lines under EGF treatment, and melatonin increased these genes’ expression in both cell lines (p<0.05). EGF increased the MMP9 and MMP2 gene expression, and melatonin increased EGF-induced expression (p<0.05). The EGF reduced the expression of the Ki67 protein in the MCF7 cell line, which was negatively affected by melatonin and EGF. In contrast, along with melatonin, EGF did not affect the proliferation of the MDA-MB-231 cell line.

Conclusions
The results of this study show that melatonin in the presence of EGF does not show the anti-cancer properties previously described for this substance.
The effect of adipose-derived stem cells (ADSC) treatment on kidney histopathological appearance on the Wistar rat models with grade five kidney trauma
Authors: Agil Ahmad , Romdam Tjahjodjati , Atik Nur , Rachmadi Dedi , Yantisetiasti Anglita , Zumrutbas Ali E. 
Published: July 2024

Abstract

Objectives
Kidney trauma is the most common urological trauma. Technological advances have made conservative management possible for almost all kidney trauma. However, grade five kidney trauma needs to be carefully examined due to its various complications, especially late complications that often delayed in recognition thus forming irreversible morbidity, with the most common late complication is kidney damage due to ischemic and fibrotic process. This study aims to confirm the effect of Adipose-Derived Stem Cells (ADSC) on the prevention of fibrosis in grade five kidney trauma using Wistar rat models, where the fibrosis process will be measured with histopathological examination which had features of glomerular sclerosis, tubular atrophy, and interstitial fibrosis in kidney tissue, then followed by histopathological scoring and total renal score.

Methods
A total of 22 adult rats were divided into five groups: one healthy control group, two trauma groups without ADSC, and two others trauma groups with ADSC. Two different treatment times were set: two weeks and four weeks after treatment. The data were tested for normality (Shapiro-Wilk test), while differences between groups were assessed using one-way ANOVA or Kruskal-Wallis test if the distribution was not normal.

Results
For the result of total renal score, statistical analysis reveal a significant difference in the total renal score in the kidney trauma with ADSC group compared with kidney trauma without ADSC group in fourth week of observation (p=0.001).

Conclusions
These findings highlighted ADSC capability to prevent fibrosis caused by grade five kidney trauma on the Wistar rat models, as proven by significantly reduced histopathological grading on fibrosis.
Composite liquids under high-power heating: superheat of water in micro-explosion of water-in-fuel droplets
Authors: Melkikh Alexey , Skripov Pavel 
Published: July 2024

Abstract
The article analyses the degree of water superheating with respect to the liquid-vapour equilibrium line in experiments on the micro-explosion of a composite droplet comprised of two immiscible liquids. The analyses were carried out for water-in-fuel drops under conditions of high-power heating. This degree is compared with the mechanical effect of droplet decay, involving the formation of daughter droplets. Our attention was drawn to the smallness of the degree of superheating preceding the decay. A model of the boiling up of such a droplet is constructed taking into account the sources of premature boiling up of water inherent in micro-explosive experiments. The dependencies of the boiling up temperature of water on the heating rate obtained in the model turned out to be in accordance with the experimental data across a wide range of heating rates. A hypothesis about the local superheating of the transition layer, which is not detected in the experiment, is formulated. Thus, a step has been taken to clarify the essence of the mismatch of the degree of superheating of water recorded by macroscopic equipment along with a completely satisfactory generation of daughter droplets serving as the basis for advanced fuel technology.