bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytocompatible and water‐stable camelina protein films for tissue engineering
Authors: Zhao Yi , Jiang Qiuran , Xu Helan , Reddy Narendra , Xu Lan , Yang Yiqi 
Published: October 2013

AbstractIn this research, films with compressive strength and aqueous stability were developed from camelina protein (CP) for tissue engineering. Protein based scaffolds have poor mechanical properties and aqueous stability and generally require chemical or physical modifications to make them applicable for medical applications. However, these modifications such as crosslinking could reduce biocompatibility and/or degradability of the scaffolds. Using proteins that are inherently water‐stable could avoid modifications and provide scaffolds with the desired properties. CP with a high degree of disulfide cross‐linkage has the potential to provide water‐stable biomaterials, but it is difficult to dissolve CP and develop scaffolds. In this study, a new method of dissolving highly cross‐linked proteins that results in limited hydrolysis and preserves the protein backbone was developed to produce water‐stable films from CP without any modification. Only 12 % weight loss of camelina films was observed after 7 days in phosphate buffer saline (PBS) at 37°C. NIH 3T3 fibroblasts could attach and proliferate better on camelina films than on citric acid cross‐linked collagen films. Therefore, CP films have the potential to be used for tissue engineering, and this extraction‐dissolution method can be used for developing biomedical materials from various water‐stable plant proteins. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 102B: 729–736, 2014.
Collagen‐Vicryl scaffolds for reconstruction of the diaphragm in a large animal model
Authors: Brouwer Katrien M. , Daamen Willeke F. , Hoogenkamp Henk R. , Geutjes Paul J. , de Blaauw Ivo , Janssen‐Kessels Wilma , de Boode Willem , Versteeg Elly , Wijnen René M. , Feitz Wout F. , Wijnen Marc , van Kuppevelt Toin H. 
Published: October 2013

AbstractCurrent methods for closure of congenital diaphragmatic hernia using patches are unsatisfactory, and novel collagen‐based scaffolds have been developed, and successfully applied in a rat model. However, for translation to the human situation constructs must be evaluated in larger animal models. We developed collagen scaffolds enforced with Vicryl, loaded either with or without the muscle stimulatory growth factor insulin‐like growth factor 1 (IGF1). We describe our steps to a surgical method to implant these scaffolds into a diaphragmatic defect in 1.5–3 week old lambs, and evaluate the scaffolds 6 months after implantation. Omentum was attached to the scaffold. At sacrifice, eventration of the implantation site was observed in all animals with a thin layer of tissue separating the abdomen from the thorax. Histologically, no scaffold remnants could be observed. Fatty tissue surrounded by fibrous tissue was seen, resembling encapsulated omentum, with collagen‐rich tissue present between this tissue and the original diaphragmatic muscle. Outcomes were not different for scaffolds with or without IGF1. In conclusion, the scaffolds integrated well into the surrounding tissue, but slower degrading materials are needed to prevent eventrations. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 102B: 756–763, 2014.
Novel paper‐templated fabrication of hierarchically porous Ni–Al layered double hydroxides/Al2O3 for efficient BSA separation
Authors: Zhang Tao , Mei Zhenyu , Zhou Yuming , Yu Shuna , Chen Zhenjie , Bu Xiaohai 
Published: October 2013

AbstractBACKGROUNDDue to cellulose of paper is abundant, inexpensive and renewable in nature, the development of paper‐based materials from cellulose to substitute materials prepared by complicated chemical routes is a fascinating challenge. This work investigates a novel method for fabricating paper‐based Ni‐Al layered double hydroxides (LDHs)/Al2O3 composite using celluloses as template; the fabricated composite was applied to adsorb bovine serum albumin (BSA) from water.RESULTSScanning electron microscopy (SEM) images demonstrate the hierarchical morphologies and multiscale structures of composites at scales ranging from micro‐ to nano‐scales. The unique macroporous structures can be directly observed by morphology analysis, and show that these materials which have potential applications in bulky biomolecules adsorption. Compared with bare LDH particles, LDHs/Al2O3 composites show a high binding capacity for BSA, where the appropriate adsorption capacity is achieved at neutral pH. The equilibrium data for BSA adsorption onto composites are found to be well described by the Langmuir isotherm model, while the kinetic data are well‐fitted to the pseudo‐second‐order model.CONCLUSIONSince the BSA adsorbed composites show near complete (over 95%) desorption in a high negative charge density of salt solution, suggesting that the paper‐based Ni‐Al LDHs/Al2O3 composites are practically usable for BSA separation. © 2013 Society of Chemical Industry
Robotic single‐site cholecystectomy
Authors: Morel Philippe , Buchs Nicolas C. , Iranmanesh Pouya , Pugin François , Buehler Leo , Azagury Dan E. , Jung Minoa , Volonte Francesco , Hagen Monika E. 
Published: October 2013

AbstractBackgroundMinimally invasive approaches for cholecystectomy are evolving in a surge for the best possible clinical outcome for the patients. As one of the most recent developments, a robotic set of instrumentation to be used with the da Vinci Si Surgical System has been developed to overcome some of the technical challenges of manual single incision laparoscopy.MethodsFrom February 2011 to February 2013, all consecutive robotic single site cholecystectomies (RSSC) were prospectively collected in a dedicated database. Demographic, intra‐ and postoperative data of all patients that underwent RSSC at our institution were analyzed. Data were evaluated for the overall patient cohort as well as after stratification according to patient BMI (body mass index) and surgeon's experience.ResultsDuring the study period, 82 patients underwent robotic single site cholecystectomy at our institution. The dominating preoperative diagnosis was cholelithiasis. Mean overall operative time was 91 min. Intraoperative complications occurred in 2.4% of cases. One conversion to open surgery due to the intraoperative finding of a gallbladder carcinoma was observed and two patients needed an additional laparoscopic trocar. The rate of postoperative complications was 4.9% with a mean length of stay of 2.4 days. No significant differences were observed when comparing results between robotic novices and robotic experts. Patients with higher BMI trended towards longer surgical console and overall operative time, but resulted in similar rates of conversions and complications when compared to normal weight patients.ConclusionsRobotic Single‐Site cholecystectomy can be performed safely and effectively with low rates of complications and conversions in patients with differing BMI and by surgeons with varying levels of experience.
Intraoperative dopamine release during globus pallidus internus stimulation in Parkinson's disease
Authors: Martinez Raquel C.R. , Hamani Clement , de Carvalho Milene Cristina , de Oliveira Amanda Ribeiro , Alho Eduardo , Navarro Jessie , dos Santos Ghilardi Maria Gabriela , Bor‐Seng‐Shu Edson , Heinsen Helmut , Otoch José Pinhata , Brandão Marcus Lira , Barbosa Egberto Reis , Teixeira Manoel Jacobsen , Fonoff Erich Talamoni 
Published: October 2013

ABSTRACTBackgroundIt is still unclear whether dopamine (DA) levels correlate with Parkinson's disease (PD) severity or play a role in the mechanisms of high‐frequency stimulation (HFS).MethodsWe have used microdialysis to record pallidal DA in 5 patients with PD undergoing microelectrode‐guided pallidotomy.ResultsWe found that patients with more severe disease and, consequently, lower pallidal DA did poorly after pallidal lesions. In the operating room, 4 of 5 patients had a significant increase in DA levels during HFS (600%, on average). To test the hypothesis that DA was important for the effects of stimulation, we correlated the amelioration in rigidity observed in the operating room with pallidal DA release. Though rigidity was 56% better during stimulation, no correlation was found between such an improvement and DA release.ConclusionsThese findings suggest that additional mechanisms not directly dependent on pallidal DA release may be involved in the clinical effects of HFS of the globus pallidus internus. © 2013 International Parkinson and Movement Disorder Society
Long‐term efficacy and safety of fluphenazine in patients with Tourette syndrome
Authors: Wijemanne Subhashie , Wu Laura J.C. , Jankovic Joseph 
Published: October 2013

ABSTRACTObjectivesHaloperidol and pimozide are the only drugs currently approved by the U.S. Food and Drug Administration for treatment of Tourette syndrome (TS), but their potential side effects, which include tardive dyskinesia (TD), limit their use.MethodsWe performed a retrospective chart review of patients with TS treated with fluphenazine over a 26‐year period.ResultsAmong 268 patients with TS, fluphenazine was initiated at a mean age of 15.8 ± 10.7 years (range, 4.1–70.2) and titrated to an optimal dose of 3.24 ± 2.3 mg/day (range, 0.5–12.0), which was continued for an average of 2.6 ± 3.2 years (range, 0.01–16.8). Marked to moderate improvement was noted in 211 (80.5%). The most common side effects included drowsiness, fatigue, or both, observed in 70 (26.1%) patients. There were no cases of TD.ConclusionsFluphenazine appears to be safe and effective in the treatment of TS, and there were no cases of TD in this cohort treated up to 16.8 years. © 2013 International Parkinson and Movement Disorder Society
1H NMR detects different metabolic profiles in glioblastoma stem‐like cells
Authors: Guidoni Laura , Ricci‐Vitiani Lucia , Rosi Antonella , Palma Alessandra , Grande Sveva , Luciani Anna Maria , Pelacchi Federica , di Martino Simona , Colosimo Cesare , Biffoni Mauro , De Maria Ruggero , Pallini Roberto , Viti Vincenza 
Published: October 2013

The metabolic profiles of glioblastoma stem‐like cells (GSCs) growing in neurospheres were examined by 1H NMR spectroscopy. Spectra of two GSC lines, labelled 1 and 83, from tumours close to the subventricular zone of the temporal lobe were studied in detail and compared with those of neural stem/progenitor cells from the adult olfactory bulb (OB‐NPCs) and of the T98G glioblastoma cell line. In both GSCs, signals from myoinositol (Myo‐I), UDP‐hexosamines (UDP‐Hex) and glycine indicated an astrocyte/glioma metabolism. For line 1, the presence of signals from N‐acetyl aspartate, GABA and creatine pointed to a neuronal fingerprint. These metabolites were almost absent from line 83 spectra, whereas lipid signals, absent from normal neural lineages, were intense in line 83 spectra and remained low in those of line 1, irrespective of apoptotic fate. Spectra of OB‐NPC cells displayed strong similarities with those from line 1, with low lipid signals and clearly detectable neuronal signals. In contrast, the spectral profile of line 83 was more similar to that of T98G, displaying high lipids and nearly complete absence of the neuronal markers. A mixed neural–astrocyte metabolic phenotype with a strong neuronal fingerprint was therefore found in line 1, while an astrocytic/glioma‐like metabolism prevailed in line 83. We found a signal assigned to the amide proton of N‐acetyl galactosamine in GSC lines and in OB‐NPC spectra, whereas it was absent from those of T98G cells. This signal may be related to a stem‐cell‐specific protein glycosylation pattern and is therefore suggested as a marker of cell multipotency.Other GSC lines from patients with different clinical outcomes were then examined. Unsupervised analysis of spectral data from 13 lines yielded two clusters, with six lines resembling spectral features of line 1 and seven resembling those of line 83, suggesting that distinct metabolic phenotypes may be present in GSC lines. Copyright © 2013 John Wiley & Sons, Ltd.
The Magic of Money and the Magic of the State: Fast Money Schemes in Papua New Guinea
Authors: Cox John 
Published: October 2013

AbstractAs Keith Hart (1986) articulated in his neat phrase ‘two sides of the coin’, money and the state are inextricably intertwined. However, academic discussions of the state tend to fall under the heading of ‘governance’, with implicit reference to democratic ideals, while money is regarded as ‘economics’, a field dominated by ideas of the market. In this paper, I use material from U‐Vistract, a mass Ponzi scam to show how quasi‐magical ideas of money and wealth have grown out of the disillusioning experience of the state in its failure to deliver ‘development’. These imaginings of prosperity entail a different kind of state, based on the moral reform of Christian citizens and political leaders and the reorientation of the banking system to deliver benefits to ordinary people. As the Royal Kingdom of Papala, U‐Vistract sought to be seen to be like a Christian state and so deceived its investors into thinking that they were participating in a moral project that would allow them to redress the short‐comings of the Papua New Guinean state. As the scam took on the appearance of the state, so the state came to be seen as a scam.
Beyond Bilas: The Enga Take Anda
Authors: Wiessner Polly , Tumu Akii 
Published: October 2013

AbstractA crucial issue facing anthropologists, linguists, and museums today is what to do with research material and artifacts that have accumulated over the years. In 2005 we sought ways to return 20 years of material on Enga history and tradition to the people of Enga Province at a time when rapid change was leaving young generations with little sense of their cultural heritage. The goal was to build a center, the Enga Take Anda, House of Traditional Knowledge, that would provide knowledge of the past to help Enga understand recent changes and to consider what should be carried forward and what left behind. To be effective, the Enga Take Anda would have to be a museum and as well as a resource center with an active role in Enga schools and the Village Court system. We describe here challenges and successes in this endeavor. The establishment of the Take Anda has involved much trial, error, flexibility and persistence, largely persistence.
Characteristics and trends of low‐dose quetiapine use in two western state Medicaid programs
Authors: Hartung Daniel M. , Zerzan Judy , Yamashita Traci , Tong Suhong , Morden Nancy E. , Libby Anne M. 
Published: October 2013

ABSTRACTPurposeMedicaid programs are concerned about inappropriate, potentially hazardous, and costly off‐label use of second‐generation antipsychotics (SGAs). Several states are exploring policies aimed at managing low‐dose quetiapine, commonly prescribed for off‐label conditions. This study aimed to characterize longitudinal trends and patient characteristics associated with low‐dose quetiapine in two state Medicaid programs. We further aimed to quantify changes in the use of quetiapine associated with a legal settlement that curtailed off‐label promotion of this product.MethodsUsing administrative data from two state Medicaid programs, Oregon and Colorado, we identified SGA initiators and determined patient level factors associated with receipt of low‐dose SGAs. We evaluated changes in low‐dose quetiapine initiation during and after a period in which quetiapine was being promoted illegally for off‐label purposes.ResultsWe identified 14 763 new SGA starts during the study period. Low‐dose (versus therapeutic dose) SGA use was common in both states, representing 53% to 56% of initiators. Quetiapine was the most commonly used SGA in both states and both dose ranges. Diagnoses of schizophrenia, bipolar disorder, posttraumatic stress disorder, anxiety disorder, and use of newer sedative hypnotics were associated with lower likelihood of initiating low‐dose quetiapine. Initiation of low‐dose quetiapine as a proportion of all SGA initiation and of all quetiapine initiation significantly declined in Oregon following suspension of off‐label promotional activities.ConclusionsLow‐dose SGA and specifically low‐dose quetiapine use remains common. Medicaid programs must set policies carefully to maximize the net safety of prescription use while optimizing disease management considering the potential for substitution effects. Copyright © 2013 John Wiley & Sons, Ltd.
Phosphido‐diphosphine pincer aluminum complexes as catalysts for ring opening polymerization of cyclic esters
Authors: D'Auria Ilaria , Lamberti Marina , Mazzeo Mina , Milione Stefano , Pellecchia Claudio 
Published: October 2013

ABSTRACTNew aluminum alkyl complexes, supported by o‐phenylene‐derived phosphido diphosphine pro‐ligands [Ph‐PPP]‐H and [iPr‐PPP]‐H ([Ph‐PPP]‐H = bis(2‐diphenylphosphinophenyl)phosphine; [iPr‐PPP]‐H = bis(2‐diisopropylphosphinophenyl)phosphine) are reported. Compounds [Ph‐PPP]AlMe2 (1), [iPr‐PPP]AlMe2 (2), and [Ph‐PPP]AliBu2 (3) have been synthesized by reaction of the pro‐ligand with the appropriate trialkyl aluminum precursor and have been characterized by 1H, 13C and 31P NMR spectroscopy. The solution NMR data and theoretical calculations suggest for all complexes trigonal bipyramidal structures with C2v symmetry in which the phosphido diphosphine ligand acts as a κ3 coordinated ligand. All complexes promote the ring‐opening polymerization of ε‐caprolactone, L‐ and rac‐lactide. Polyesters with controlled molecular parameters (Mn, end groups) and low polydispersities are obtained. Upon addition of isopropanol, efficient binary catalytic systems for the immortal ring‐opening polymerization of the cyclic esters are produced. Preliminary investigations show the ability of these complexes to promote copolymerization of l‐lactide and ɛ‐caprolactone to achieve copolymers whose microstructures are depending on the structure of the catalyst. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2014, 52, 49–60
Preferential N‐H bond direction in GaAsN grown by chemical beam epitaxy
Authors: Ikeda Kazuma , Inagaki Makoto , Kojima Nobuaki , Ohshita Yoshio , Yamaguchi Masafumi 
Published: October 2013

AbstractThe two N‐H local vibrations in GaAsN grown by the chemical beam epitaxy have the same preferential orientation in the crystal. The bond orientations of the N‐H wagging mode at 961 cm‐1 and stretching mode at 2952 cm‐1 tend to align along [1‐10]. The peak intensities of the three major N‐H local vibration modes were examined by the polarized Fourier transform infrared spectroscopy. The integrated absorption peaks at 961and 2952 cm‐1 showed the two‐fold rotational symmetry in (001) plane, while the one at 3098 cm‐1 was almost constant within the accuracy of this measurement. The maximum peak intensities were observed when the IR polarization direction was [110] for the N‐H wagging mode at 961 cm‐1, and [1‐10] for the N‐H stretching mode at 2952 cm‐1, which means that those bond orientations projected on (001) plane were the same. The result indicates that the N‐H wagging mode at 961 cm‐1 and stretching mode at 2952 cm‐1 are originated from the same N‐H bond. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Growth of high‐quality InAs quantum dots embedded in GaAs nanowire structures on Si substrates
Authors: Kwoen Jinkwan , Watanabe Katsuyuki , Ota Yasutomo , Iwamoto Satoshi , Arakawa Yasuhiko 
Published: October 2013

AbstractWe present the growth of InAs quantum dots (QD) embedded in GaAs nanowires (NW) on silicon and demonstrate clear single photon emission. Sharp excitonic emission (linewidth of 162 μeV) and the generation of single photons (g(2)(0) = 0.18) was observed by low temperature photoluminescence measurements, suggesting a high optical quality which can be attributed to the high crystal quality of the QD structure. It is believed that the crystal quality is high due to the Stranski‐Krastanov (S‐K) mode of the growth. This structure may open new ways for the integration of such photon emitters into future ‘on‐silicon’ quantum optical circuits. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Observation of CO2 and solvent adduct ions during negative mode electrospray ionization Fourier transform ion cyclotron resonance mass spectrometric analysis of monohydric alcohols
Authors: Zhou Xibin , Zhang Yahe , Zhao Suoqi , Hsu Chang Samuel , Shi Quan 
Published: October 2013

RATIONALEMonohydric alcohols are common in natural products, bio‐oils, and medicine. We have found that monohydric alcohols can form O3 (ions containing three oxygen atoms) and O4 adduct ions in negative electrospray ionization (ESI) Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR MS), which would significantly affect the composition analysis of alcohols, especially in a complex mixture. It is necessary to study the reaction pathways and the method to eliminate or reduce the 'artifact' adducts.METHODSOctadecanol, cholesterol, squalanol and two complex monohydric alcohol mixtures were selected as model compounds. These samples were subjected to negative ion ESI FT‐ICR MS analysis. The composition and formation mechanism of adducts were studied by the ultrahigh‐resolution accurate mass measurement for elemental composition, along with the MS2 isolation and collision‐induced dissociation (CID) experiments for structural determination.RESULTSThe reaction pathway of O3 adduct formation is the coupling of a monohydric alcohol ion with a CO2 to form a stable O3 ionic species by likely a covalent bond (source of CO2 is not clear). The O4 species are formed by O3 ionic species adducted with an alcohol molecule of the solvent, such as methanol or ethanol, by likely a hydrogen bond. These adduct ions could be eliminated or reduced by increasing collision energy. However, excessive collision energy would fragment monohydric alcohol ions.CONCLUSIONSThe formation mechanisms of O3 and O4 adducts from monohydric alcohols in negative ion ESI FT‐ICR MS were proposed. The solvent adduction effects can be eliminated or reduced by optimizing the collision energy of CID in FT‐ICR MS. Copyright © 2013 John Wiley & Sons, Ltd.