bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic changes in amygdala activation and functional connectivity in children and adolescents with anxiety disorders
Authors: Swartz Johnna R. , Phan K. Luan , Angstadt Mike , Fitzgerald Kate D. , Monk Christopher S. 
Published: November 2014

AbstractAnxiety disorders are associated with abnormalities in amygdala function and prefrontal cortex–amygdala connectivity. The majority of functional magnetic resonance imaging studies have examined mean group differences in amygdala activation or connectivity in children and adolescents with anxiety disorders relative to controls, but emerging evidence suggests that abnormalities in amygdala function are dependent on the timing of the task and may vary across the course of a scanning session. The goal of the present study was to extend our knowledge of the dynamics of amygdala dysfunction by examining whether changes in amygdala activation and connectivity over scanning differ in pediatric anxiety disorder patients relative to typically developing controls during an emotion processing task. Examining changes in activation over time allows for a comparison of how brain function differs during initial exposure to novel stimuli versus more prolonged exposure. Participants included 34 anxiety disorder patients and 19 controls 7 to 19 years old. Participants performed an emotional face-matching task during functional magnetic resonance imaging scanning, and the task was divided into thirds in order to examine change in activation over time. Results demonstrated that patients exhibited an abnormal pattern of amygdala activation characterized by an initially heightened amygdala response relative to controls at the beginning of scanning, followed by significant decreases in activation over time. In addition, controls evidenced greater context-modulated prefrontal cortex–amygdala connectivity during the beginning of scanning relative to patients. These results indicate that differences in emotion processing between the groups vary from initial exposure to novel stimuli relative to more prolonged exposure. Implications are discussed regarding how this pattern of neural activation may relate to altered early-occurring or anticipatory emotion-regulation strategies and maladaptive later-occurring strategies in children and adolescents with anxiety disorders.
Regulating sadness and fear from outside and within: Mothers' emotion socialization and adolescents' parasympathetic regulation predict the development of internalizing difficulties
Authors: Hastings Paul D. , Klimes-Dougan Bonnie , Kendziora Kimberly T. , Brand Ann , Zahn-Waxler Carolyn 
Published: November 2014

AbstractMultilevel models of developmental psychopathology implicate both characteristics of the individual and their rearing environment in the etiology of internalizing problems and disorders. Maladaptive regulation of fear and sadness, the core of anxiety and depression, arises from the conjoint influences of ineffective parasympathetic regulation of emotion and ineffective emotion socialization experiences. In 171 youths (84 female,M= 13.69 years,SD= 1.84), we measured changes of respiratory sinus arrhythmia (RSA) in response to sadness- and fear-inducing film clips and maternal supportive and punitive responses to youths' internalizing emotions. Youths and mothers reported on youths' internalizing problems and anxiety and depression symptoms concurrently and 2 years later at Time 2. Maternal supportive emotion socialization predicted fewer, and punitive socialization predicted more, mother-reported internalizing problems at Time 2 only for youths who showed RSA suppression to fear-inducing films. More RSA suppression to sadness-inducing films predicted more youth-reported internalizing problems at Time 2 in girls only. In addition, less supportive emotion socialization predicted more youth-reported depression symptoms at Time 2 only for girls who showed more RSA suppression to sadness. RSA suppression to sadness versus fear might reflect different patterns of atypical parasympathetic regulation of emotional arousal, both of which increase the risk for internalizing difficulties in youths, and especially girls, who lack maternal support for regulating emotions.
Two prospective studies of changes in stress generation across depressive episodes in adolescents and emerging adults
Authors: Morris Matthew C. , Kouros Chrystyna D. , Hellman Natalie , Rao Uma , Garber Judy 
Published: November 2014

AbstractThe stress generation hypothesis was tested in two different longitudinal studies examining relations between weekly depression symptom ratings and stress levels in adolescents and emerging adults at varied risk for depression. The participants in Study 1 included 240 adolescents who differed with regard to their mothers' history of depressive disorders. Youth were assessed annually across 6 years (Grades 6–12). Consistent with the depression autonomy model, higher numbers of prior major depressive episodes (MDEs) were associated with weaker stress generation effects, such that higher levels of depressive symptoms predicted increases in levels of dependent stressors for adolescents with two or more prior MDEs, but depressive symptoms were not significantly related to dependent stress levels for youth with three or more prior MDEs. In Study 2, the participants were 32 remitted-depressed and 36 never-depressed young adults who completed a psychosocial stress task to determine cortisol reactivity and were reassessed for depression and stress approximately 8 months later. Stress generation effects were moderated by cortisol responses to a laboratory psychosocial stressor, such that individuals with higher cortisol responses exhibited a pattern consistent with the depression autonomy model, whereas individuals with lower cortisol responses showed a pattern more consistent with the depression sensitization model. Finally, comparing across the two samples, stress generation effects were weaker for older participants and for those with more prior MDEs. The complex, multifactorial relation between stress and depression is discussed.
Coping with having a depressed mother: The role of stress and coping in hypothalamic–pituitary–adrenal axis dysfunction in girls at familial risk for major depression
Authors: Foland-Ross Lara C. , Kircanski Katharina , Gotlib Ian H. 
Published: November 2014

AbstractHaving a depressed mother is one of the strongest predictors of depression in adolescence. We investigated whether the stress of having a mother with recurrent depression is associated with dysfunction in adolescents in the HPA axis and whether the tendency to use involuntary coping strategies in dealing with this stress is associated with exacerbation of dysfunction in this system. Sixty-four never-disordered daughters of mothers with recurrent depression (high risk) and 64 never-disordered daughters of never-disordered mothers (low risk) completed diurnal cortisol and stress assessments. High-risk girls secreted more diurnal cortisol than did low-risk girls. Whereas low-risk girls secreted higher levels of cortisol with increasing stress associated with having a depressed mother, no such relation was present in high-risk girls. Finally, in contrast to low-risk girls, girls at familial risk for depression who more frequently used involuntary versus voluntary coping exhibited the greatest elevations in diurnal cortisol. These findings indicate that a tendency to utilize involuntary, as opposed to voluntary, coping strategies in dealing with stress involving maternal depression exacerbates already high levels of cortisol in youth at risk for depression. Future research that examines whether interventions aimed at increasing the use of voluntary coping strategies normalizes HPA axis dysfunction is of interest.
Occupational Risk Factors in the Emergency Medical Services
Authors: Donnelly Elizabeth , Siebert Darcy 
Published: November 2014

AbstractIntroduction:During a medical emergency, the American public often relies on the expertise of emergency medical technicians (EMTs). These professionals face a number of occupational hazards, and the literature suggests that EMTs are at a greater risk of developing physical and mental stress-related disorders. The purpose of this paper is to systematically examine gaps in the extant literature and to present a theoretically driven conceptual model to serve as a basis for future intervention and research efforts.Methods:A systematic review of the literature was conducted utilizing rele-vant databases (e.g., PsychInfo, Medline). All empirical articles regarding emergency medical responders were reviewed, but given the limited research available, relevant theoretical and conceptual literature on the constructs of interest in other, related populations also were included. Based on this extensive review, a modification of the stress process model is suggested to explain the relationships among occupational stress exposure, post-traumatic stress disorder (PTSD), and high-risk alcohol and other drug use.Results:Exposure to traumatic events was reported to be between 80% and 100% among EMTs, and rates of PTSD are >20%. High-risk alcohol and drug use rates among other emergency response professionals were reported to be as high as 40%. The proposed model suggests direct linkages between occupationally related stress exposure, including chronic and critical incident stress, PTSD, and high-risk alcohol and other drug use. Social support and personal resources (e.g., coping, locus of control) are proposed to have mediating and moderating influences on the three main constructs, and cohesion is introduced as an important, idiosyncratic influence in this population. The moderating influences of gender, age, ethnicity, marital status, and socioeconomic status, level of training, and years of service are included in the proposed model.Conclusions:High-risk alcohol and other drug use and post-traumatic symptomatology pose substantial risks for EMTs, and consequently, for the patients they serve. It is imperative that researchers develop and test a theoretically grounded model of risk and protective factors that will guide intervention development and future study. The model suggested in this paper, based on a comprehensive literature review and development of theory, represents a critical first step in the intervention research process.
Environmental metabarcoding reveals heterogeneous drivers of microbial eukaryote diversity in contrasting estuarine ecosystems
Authors: Lallias Delphine , Hiddink Jan G , Fonseca Vera G , Gaspar John M , Sung Way , Neill Simon P , Barnes Natalie , Ferrero Tim , Hall Neil , Lambshead P John D , Packer Margaret , Thomas W Kelley , Creer Simon 
Published: November 2014

Abstract
Assessing how natural environmental drivers affect biodiversity underpins our understanding of the relationships between complex biotic and ecological factors in natural ecosystems. Of all ecosystems, anthropogenically important estuaries represent a ‘melting pot’ of environmental stressors, typified by extreme salinity variations and associated biological complexity. Although existing models attempt to predict macroorganismal diversity over estuarine salinity gradients, attempts to model microbial biodiversity are limited for eukaryotes. Although diatoms commonly feature as bioindicator species, additional microbial eukaryotes represent a huge resource for assessing ecosystem health. Of these, meiofaunal communities may represent the optimal compromise between functional diversity that can be assessed using morphology and phenotype–environment interactions as compared with smaller life fractions. Here, using 454 Roche sequencing of the 18S nSSU barcode we investigate which of the local natural drivers are most strongly associated with microbial metazoan and sampled protist diversity across the full salinity gradient of the estuarine ecosystem. In order to investigate potential variation at the ecosystem scale, we compare two geographically proximate estuaries (Thames and Mersey, UK) with contrasting histories of anthropogenic stress. The data show that although community turnover is likely to be predictable, taxa are likely to respond to different environmental drivers and, in particular, hydrodynamics, salinity range and granulometry, according to varied life-history characteristics. At the ecosystem level, communities exhibited patterns of estuary-specific similarity within different salinity range habitats, highlighting the environmental sequencing biomonitoring potential of meiofauna, dispersal effects or both.
Significance of archaeal nitrification in hypoxic waters of the Baltic Sea
Authors: Berg Carlo , Vandieken Verona , Thamdrup Bo , Jürgens Klaus 
Published: November 2014

Abstract
Ammonia-oxidizing archaea (AOA) of the phylum Thaumarchaeota are widespread, and their abundance in many terrestrial and aquatic ecosystems suggests a prominent role in nitrification. AOA also occur in high numbers in oxygen-deficient marine environments, such as the pelagic redox gradients of the central Baltic Sea; however, data on archaeal nitrification rates are scarce and little is known about the factors, for example sulfide, that regulate nitrification in this system. In the present work, we assessed the contribution of AOA to ammonia oxidation rates in Baltic deep basins and elucidated the impact of sulfide on this process. Rate measurements with 15N-labeled ammonium, CO2 dark fixation measurements and quantification of AOA by catalyzed reporter deposition–fluorescence in situ hybridization revealed that among the three investigated sites the highest potential nitrification rates (122–884 nmol l−1per day) were measured within gradients of decreasing oxygen, where thaumarchaeotal abundance was maximal (2.5–6.9 × 105 cells per ml) and CO2 fixation elevated. In the presence of the archaeal-specific inhibitor GC7, nitrification was reduced by 86–100%, confirming the assumed dominance of AOA in this process. In samples spiked with sulfide at concentrations similar to those of in situ conditions, nitrification activity was inhibited but persisted at reduced rates. This result together with the substantial nitrification potential detected in sulfidic waters suggests the tolerance of AOA to periodic mixing of anoxic and sulfidic waters. It begs the question of whether the globally distributed Thaumarchaeota respond similarly in other stratified water columns or whether the observed robustness against sulfide is a specific feature of the thaumarchaeotal subcluster present in the Baltic Deeps.
Exosomes secreted by nematode parasites transfer small RNAs to mammalian cells and modulate innate immunity
Authors: Buck Amy H. , Coakley Gillian , Simbari Fabio , McSorley Henry J. , Quintana Juan F. , Le Bihan Thierry , Kumar Sujai , Abreu-Goodger Cei , Lear Marissa , Harcus Yvonne , Ceroni Alessandro , Babayan Simon A. , Blaxter Mark , Ivens Alasdair , Maizels Rick M. 
Published: November 2014

AbstractIn mammalian systems RNA can move between cells via vesicles. Here we demonstrate that the gastrointestinal nematodeHeligmosomoides polygyrus, which infects mice, secretes vesicles containing microRNAs (miRNAs) and Y RNAs as well as a nematode Argonaute protein. These vesicles are of intestinal origin and are enriched for homologues of mammalian exosome proteins. Administration of the nematode exosomes to mice suppresses Type 2 innate responses and eosinophilia induced by the allergenAlternaria.Microarray analysis of mouse cells incubated with nematode exosomesin vitroidentifies Il33r and Dusp1 as suppressed genes, and Dusp1 can be repressed by nematode miRNAs based on a reporter assay. We further identify miRNAs from the filarial nematodeLitomosoides sigmodontisin the serum of infected mice, suggesting that miRNA secretion into host tissues is conserved among parasitic nematodes. These results reveal exosomes as another mechanism by which helminths manipulate their hosts and provide a mechanistic framework for RNA transfer between animal species.
Ultralow mode-volume photonic crystal nanobeam cavities for high-efficiency coupling to individual carbon nanotube emitters
Authors: Miura R. , Imamura S. , Ohta R. , Ishii A. , Liu X. , Shimada T. , Iwamoto S. , Arakawa Y. , Kato Y. K. 
Published: November 2014

AbstractThe unique emission properties of single-walled carbon nanotubes are attractive for achieving increased functionality in integrated photonics. In addition to being room-temperature telecom-band emitters that can be directly grown on silicon, they are ideal for coupling to nanoscale photonic structures. Here we report on high-efficiency coupling of individual air-suspended carbon nanotubes to silicon photonic crystal nanobeam cavities. Photoluminescence images of dielectric- and air-mode cavities reflect their distinctly different mode profiles and show that fields in the air are important for coupling. We find that the air-mode cavities couple more efficiently, and estimated spontaneous emission coupling factors reach a value as high as 0.85. Our results demonstrate advantages of ultralow mode-volumes in air-mode cavities for coupling to low-dimensional nanoscale emitters.
Loss of PHD3 allows tumours to overcome hypoxic growth inhibition and sustain proliferation through EGFR
Authors: Henze Anne-Theres , Garvalov Boyan K. , Seidel Sascha , Cuesta Angel M. , Ritter Mathias , Filatova Alina , Foss Franziska , Dopeso Higinio , Essmann Clara L. , Maxwell Patrick H. , Reifenberger Guido , Carmeliet Peter , Acker-Palmer Amparo , Acker Till 
Published: November 2014

AbstractSolid tumours are exposed to microenvironmental factors such as hypoxia that normally inhibit cell growth. However, tumour cells are capable of counteracting these signals through mechanisms that are largely unknown. Here we show that the prolyl hydroxylase PHD3 restrains tumour growth in response to microenvironmental cues through the control of EGFR. PHD3 silencing in human gliomas or genetic deletion in a murine high-grade astrocytoma model markedly promotes tumour growth and the ability of tumours to continue growing under unfavourable conditions. The growth-suppressive function of PHD3 is independent of the established PHD3 targets HIF and NF-κB and its hydroxylase activity. Instead, loss of PHD3 results in hyperphosphorylation of epidermal growth factor receptor (EGFR). Importantly, epigenetic/genetic silencing of PHD3 preferentially occurs in gliomas without EGFR amplification. Our findings reveal that PHD3 inactivation provides an alternative route of EGFR activation through which tumour cells sustain proliferative signalling even under conditions of limited oxygen availability.
Disruption of FAT10–MAD2 binding inhibits tumor progression
Authors: Theng Steven Setiawan , Wang Wei , Mah Way-Champ , Chan Cheryl , Zhuo Jingli , Gao Yun , Qin Haina , Lim Liangzhong , Chong Samuel S. , Song Jianxing , Lee Caroline G. 
Published: November 2014

Significance
FAT10, a ubiquitin-like modifier, is an oncogene that interacts with mitotic arrest-deficient 2 (MAD2) and confers cellular malignancy. Here we identified the MAD2-binding residues of FAT10 and determined the first solution structure, to our knowledge, of the first FAT10 ubiquitin-like domain. Importantly, we demonstrated the proof-of-mechanism for a novel and specific drug-targeting strategy that entails the specific inhibition of the pathological activity of a therapeutic target but not its reported physiological function, thus minimizing undesirable side effects: Abrogation of the FAT10–MAD2 interaction curtailed tumor progression without affecting FAT10’s interaction with its other known physiological binding partners. This study presents a paradigm for drug targeting and paves the way for the development of a novel small-molecule anticancer inhibitor targeting the MAD2-binding interface of FAT10.
Mechanism of ganciclovir-induced chain termination revealed by resistant viral polymerase mutants with reduced exonuclease activity
Authors: Chen Han , Beardsley G. Peter , Coen Donald M. 
Published: November 2014

Significance
Nucleoside analogues include important drugs that target DNA polymerases and cause chain termination. However, how ganciclovir, the first line of treatment for human cytomegalovirus infections, does this is unknown. Ganciclovir resistance is a serious clinical problem. Many ganciclovir-resistant isolates contain substitutions in the 3′–5′ exonuclease domain of the catalytic subunit of viral DNA polymerase. How these mutations confer resistance is a long-standing question. This study shows that both wild-type and exonuclease mutant polymerases incorporate ganciclovir into DNA and continue synthesis, but whereas the wild-type enzyme excises nucleotides two positions downstream of incorporated ganciclovir, the mutant enzymes do not, permitting chain extension. These results show how a therapeutically important drug causes chain termination and explain an unusual mechanism of drug resistance.
Functionally distinct
ERAP1
allotype combinations distinguish individuals with Ankylosing Spondylitis
Authors: Reeves Emma , Colebatch-Bourn Alexandra , Elliott Tim , Edwards Christopher J. , James Edward 
Published: November 2014

Significance
The immune system performs surveillance to identify infected or cancerous cells through recognition of small protein fragments called antigenic peptides on their surface. To do this, the peptides must be cut to a specific length by an enzyme called endoplasmic reticulum aminopeptidase 1 (ERAP1). Variation in this enzyme has recently been linked to the inflammatory rheumatic disease Ankylosing Spondylitis (AS). We have found that ERAP1 is highly polymorphic in humans and that specific combinations of ERAP1 are found in people with AS. These disease-associated combinations have a reduced ability to generate peptides for presentation at the cell surface by MHC class I molecules, including HLA-B27. Understanding this finding may allow easier identification of individuals with AS and allow stratification into prognostic groups.
Elevated hippocampal resting-state connectivity underlies deficient neurocognitive function in aging
Authors: Salami Alireza , Pudas Sara , Nyberg Lars 
Published: November 2014

SignificanceAging is accompanied by disruptive alterations in large-scale brain systems, such as the default mode network (DMN) and the associated hippocampus (HC) subsystem, which support higher cognitive functions. However, the exact form of DMN–HC alterations and concomitant memory deficits is largely unknown. We identified age-related decrements in resting-state functional connectivity of the cortical DMN, whereas elevated connectivity between the bilateral HC was found along with attenuated HC–cortical connectivity. Critically, elevated HC at rest restricts the degree to which HC interacts with other brain regions during memory tasks, and thus results in memory deficits. This study provides empirical evidence of how the relationship between the DMN and HC breaks down in aging and how such alterations underlie deficient mnemonic processing.
Actin stress in cell reprogramming
Authors: Guo Jun , Wang Yuexiu , Sachs Frederick , Meng Fanjie 
Published: November 2014

Significance
Mechanical signaling plays many roles in cell physiology, including stem cell differentiation and reprogramming. To better understand the roles of mechanical forces in stem cells, we created genetically coded probes for actin that enables the direct measurement of forces in actin in living cells. This is the first force probe created for oligomeric proteins. We reprogrammed HEK-293 and Madin-Darby canine kidney cells into stem-like cells by culturing them on a soft substrate without using transcription factors. The mechanical properties of the microenvironment, and thus the local forces, promote cell reprogramming. Surprisingly, the cells showed close association of stemness to high tension in actin. Given the universal presence of actin in animal cells, the actin force probes have broad applications in biology.