bluuebunny/crossref_metadata_2025_split huggingface dataset:
First Report of Chrysanthemum stem necrosis virus on Russell Prairie Gentian in Brazil
Authors: Duarte L. M. L. , Alexandre M. A. V. , Gobatto D. , Kitajima E. W. , Harakava R. 
Published: August 2013

In November 2012, plants of Russell prairie gentian (Eustoma grandiflorum, Lisianthus russellianus) were collected from a commercial greenhouse in Atibaia, SP, Brazil, displaying necrotic spots on leaves and necrosis on stems, followed by generalized systemic necrosis. Disease symptom incidence was estimated at 10%. Preliminary electron microscopy observations of negatively stained leaf extracts prepared from those lesions revealed the presence of a large number of spherical tospovirus-like, approximately 100 nm in diameter. Samples of infected leaves were ground in 0.01 M phosphate buffer containing 0.5% sodium sulphide and mechanically inoculated in six plants of each species of Nicotiana glutinosa, N. tabacum cv. White Burley, N. megalosiphon, N. debneyii, Datura stramonium, Chenopodium amaranticolor, C. quinoa, and E. grandiflorum. All inoculated plants displayed local lesions 4 to 5 days after inoculation, while N. debneyii and D. stramonium showed systemic symptoms, typical of Tospovirus infection. In addition, E. grandiflorum reproduced the original symptoms. Total RNA was extracted from infected E. grandiflorum and D. stramonium, and reverse transcription (RT)-PCR was performed using universal primers BR60 and BR65 (2) targeting conserved regions of the nucleocapsid gene (N). The amplification products of approximately 450 bp were purified, cloned, and sequenced. The unknown virus was identified as Chrysanthemum stem necrosis virus (CSNV-Lis) based on host range and nucleotide sequence (Genbank Accession No. KC894721) and showed 99% identity with a CSNV chrysanthemum isolate from Japan (AB600872). Maximum likelihood phylogenetic analysis using nine homologous CSNV sequences available in GenBank classified CSNV-Lis into a monophyletic group formed by chrysanthemum isolates from Japan and China while a Japanese lisianthus isolate was separately clustered. CSNV is a member of the genus Tospovirus (Bunyaviridae) and was first reported on chrysanthemum in Brazil (1) and later in the Netherlands, Slovenia, United Kingdom, and Japan (3). Despite scattered recent reports of CSNV, the simultaneous production of chrysanthemum and lisianthus crops along the year by Brazilian farmers has contributed to the virus maintenance in the field. The high identity between Brazilian and Japanese isolates of CSNV suggest a possible reintroduction of the virus through exchange of vegetative propagating material. References: (1) L. M. L. Duarte et al. J. Phytopathol. 143:569, 1995. (2) M. Eiras et al. Fitopatol. Bras. 26:170, 2001. (3) K. Momonoi et al. J. Gen. Plant Pathol. 77:142, 2011.
Arabidopsis Tetraspanins Are Confined to Discrete Expression Domains and Cell Types in Reproductive Tissues and Form Homo- and Heterodimers When Expressed in Yeast
Authors: Boavida Leonor C. , Qin Peng , Broz Miranda , Becker Jörg D. , McCormick Sheila 
Published: August 2013

AbstractTetraspanins are evolutionary conserved transmembrane proteins present in all multicellular organisms. In animals, they are known to act as central organizers of membrane complexes and thought to facilitate diverse biological processes, such as cell proliferation, movement, adhesion, and fusion. The genome of Arabidopsis (Arabidopsis thaliana) encodes 17 members of the tetraspanin family; however, little is known about their functions in plant development. Here, we analyzed their phylogeny, protein topology, and domain structure and surveyed their expression and localization patterns in reproductive tissues. We show that, despite their low sequence identity with metazoan tetraspanins, plant tetraspanins display the typical structural topology and most signature features of tetraspanins in other multicellular organisms. Arabidopsis tetraspanins are expressed in diverse tissue domains or cell types in reproductive tissues, and some accumulate at the highest levels in response to pollination in the transmitting tract and stigma, male and female gametophytes and gametes. Arabidopsis tetraspanins are preferentially targeted to the plasma membrane, and they variously associate with specialized membrane domains, in a polarized fashion, to intercellular contacts or plasmodesmata. A membrane-based yeast (Saccharomyces cerevisiae) two-hybrid system established that tetraspanins can physically interact, forming homo- and heterodimer complexes. These results, together with a likely genetic redundancy, suggest that, similar to their metazoan counterparts, plant tetraspanins might be involved in facilitating intercellular communication, whose functions might be determined by the composition of tetraspanin complexes and their binding partners at the cell surface of specific cell types.
Rate‐dependent model of structural concrete incorporating kinematics of ambient water subjected to high‐cycle loads
Authors: Maekawa Koichi , Fujiyama Chikako 
Published: August 2013

PurposeThe paper aims to propose a rate‐dependent model of structural concrete in combination with the kinematics of condensed water.Design/methodology/approachFirst, the paper proposes the coupling model of water versus cracked concrete with a mathematical completeness of equilibrium and deformational compatibility. The proposed model deals with anisotropy of structural performance and of permeability, which is a particular issue of concrete caused by cracks. The governing equation for saturated concrete in this study is based on Biot's theory that deals with particle assembly as a two‐phase composite. Second, the paper shows the possible reduction of the fatigue life of real‐scale bridge RC decks owing to the water residing in structural cracks under moving wheel‐type loading.FindingsThe paper shows that the existence of water possibly has an influence on the rate‐dependency of structural performance. The comparison of transition of pore pressure and principal strain indicates that damage to the concrete skeleton is accelerated by internal stress caused by high pore pressure. It suggests that the existence of water can reduce the fatigue life of bridge decks, especially when the upper layer is saturated.Originality/valueThis paper clarifies the effect of pore water on structural concrete by using numerical model considering kinematics of water.
Are all internships beneficial learning experiences? An exploratory study
Authors: Holyoak Lynda 
Published: August 2013

PurposeInternships are becoming a more common feature of the career paths of young people, however there has been little consideration of how people learn on internships. The purpose of this paper is report on the experiences of interns and their supervisors on a research internship programme.Design/methodology/approachThis study analyses the experiences of interns and their supervisors on a research internship programme, using communities of practice as a theoretical framework. In total, six interns and six supervisors were interviewed approximately six to eight months after the internships were completed.FindingsExtreme types of experience in the internships are illustrated by four cases derived from the interviews: optimal conditions for development; intern non‐development; supervisor frustration; and mutual dissatisfaction.Research limitations/implicationsAlthough the research is exploratory and limited to the experience of a small group of interns and their supervisors in one setting, it suggests that characteristics of the intern (such as motivation to learn) and the supervisor (e.g. willingness to provide support) can reduce or enhance the learning and development that is achieved during internships.Originality/valueThis paper questions implicit assumptions that internships are always positive and valuable learning experiences.
A critical account of practice within an innovative Foundation degree in UK higher education
Authors: Smith Paul , Poppitt Nicola , Scott Jonathan 
Published: August 2013

PurposeThe purpose of this paper is to explore the views of academics, senior university managers and employer representatives on the design and implementation of an innovative work‐based learning (WBL) programme in UK higher education.Design/methodology/approachThe research project draws upon case study research and utilizes data from semi‐structured interviews and documentary analysis of policy papers. The experiences of university staff and employer representatives were analysed methodologically using the organisational story‐telling framework.FindingsThe paper exposes voices which are rarely heard in the public domain and provides insights into the challenges involved in introducing new practices within higher education. The themes that have emerged are: the intensity of the learning experience; tensions amongst academics delivering the programme; and, academic support for students, particularly in relation to the importance of face‐to‐face contact.Originality/valueThe paper addresses key gaps in the WBL literature, most notably a dearth of critical accounts of practice within WBL programmes from the perspective of university staff and employer representatives. This type of research is also needed given the increased priority by governments in relation to forging links between business and higher education.
Is the Middle East the land of the future? It is not a given!
Authors: Madichie Nnamdi O. 
Published: August 2013

PurposeThe purpose of this article is to be a conceptual contribution to the special issue on “Is the Middle East the land of the future?”. It aims to provide a holistic picture of the efforts of the Middle East towards achieving the Millennium Development Goals (MDGs), using investments in sports, as a galvanizing force. The paper concludes that, on the balance of probability, this might not be a given.Design/methodology/approachThe paper is based on personal observations and documentary analysis of numerous studies including those in this special issue.FindingsAlthough the Middle East has made “front page” news on most global media platforms – albeit for a variety of reasons, there are signs not to be too optimistic as to whether this geographic entity is, by default, the land of the future.Research limitations/implicationsAs a conceptual piece, this article cannot be generalized across the expanse of what constitutes the Middle East. Most of the observations are based on the most boisterous in the region – notably members of the Gulf Cooperation countries (GCC) and Turkey with its rising regional influence.Practical implicationsWhile the Middle East may have been in the global spotlight, there remain challenges that cannot be so easily wished away. One such is the inability to leverage the investment in sports in a sustainable global partnership that spills over into other MDGs. There are persuasive arguments for deeper integration rather than what seems to be the fragmented sibling rivalry across the Middle East.Originality/valueThis study provides a holistic framework for analyzing a region that has been bedeviled with unrest in recent years; and one that has, to a very large extent, weathered the storm of the Arab Spring and attracted global attention.
A comparison of the effect of logistic strategy and logistics integration on firm competitiveness in the USA and China
Authors: Spillan John E. , McGinnis Michael A. , Kara Ali , Liu Yi George 
Published: August 2013

PurposeThe purpose of this manuscript is to empirically compare logistics strategies in Chinese and US manufacturing firms and the outcomes of these strategies to test the underlying factor structure and measurement equivalences of Bowersox/Daugherty model and its relationship with critical success factors.Design/methodology/approachA structured questionnaire was used to gather data from Chinese and American logistics managers. Using confirmatory factor analysis (CFA), the authors compared the three dimensions of the overall logistics strategy (OLS) – process strategy, market strategy, and information strategy – in two countries. A structural equation model (SEM) was then used to assess the impact of OLS on perceived competitiveness in two countries.FindingsAlthough the economic, political, and cultural dimensions of the two countries differed substantially, the findings were similar. Data from both countries provided strong support for the three dimensions of overall logistic strategy (OLS). In addition, it was found that OLS, when combined with logistics coordination effectiveness (LCE) and customer service effectiveness (CSE), contributes to organizational effectiveness (COMP).Practical implicationsThis research provides insights into comparative logistics in two large disparate economies and provides support for the Bowersox/Daugherty logistics/supply chain management typology.Originality/valueThis manuscript provides insights into comparative logistics/supply chain management that have not been previously reported through empirical research.
What drives environmental sustainability in the New Zealand wine industry?
Authors: Dodds Rachel , Graci Sonya , Ko Soyoung , Walker Lindsay 
Published: August 2013

PurposeWhile global wine consumption is growing, environmental sustainability in the wine industry is also receiving increased attention from retailers, governments, environmental groups, and consumers. New Zealand has experienced a winery boom over the past two decades with a 173 per cent increase in the number of wineries. Along with this growth, wineries are also facing issues such as water consumption, its impact on community, and waste management. The study therefore seeks to examine: the current sustainability initiatives undertaken by wineries; what drives the wine industry to engage in sustainable practices; and barriers to implementing sustainable practices.Design/methodology/approachUsing a mixed method research approach, this study uses a triangulated approach to examine interviews and questionnaires to determine motivations and elements of influence.FindingsThis study found that the strong drivers for the sustainability initiatives are their concern about the state of the environment and social responsibility, followed by requirements for exporting and protection of agricultural land.Research limitations/implicationsA limitation of this study was a low response rate from the online questionnaire, which may influence or bias study results. However, detailed face‐to‐face interviews help to gain clarification on concepts and study results.Practical implicationsWhile global wine consumption is growing, environmental sustainability in the wine industry is also receiving increasing attention from retailers, governments, environmental groups and consumers. New Zealand has experienced a winery boom over the past two decades with a 173 per cent increase in the number of wineries. Along with this growth, wineries are also facing issues such as water consumption, its impact on community, and waste management.Originality/valueAlthough there are many studies about the wine industry, relatively few studies have examined sustainability elements from a mixed‐method approach to determine practical elements which may influence practices undertaken. It also examines practices undertaken by wineries, barriers to implementation and incentives to further implementation.
Overlapping factors in search engine optimization and web accessibility
Authors: Moreno Lourdes , Martinez Paloma 
Published: August 2013

PurposeThe purpose of this paper is to show that the pursuit of a high search engine relevance ranking for a webpage is not necessarily incompatible with the pursuit of web accessibility.Design/methodology/approachThe research described arose from an investigation into the observed phenomenon that pages from accessible websites regularly appear near the top of search engine (such as Google) results, without any deliberate effort having been made through the application of search engine optimization (SEO) techniques to achieve this. The reasons for this phenomenon appear to be found in the numerous similarities and overlapping characteristics between SEO factors and web accessibility guidelines. Context is provided through a review of sources including accessibility standards and relevant SEO studies and the relationship between SEO and web accessibility is described. The particular overlapping factors between the two are identified and the precise nature of the overlaps is explained in greater detail.FindingsThe available literature provides firm evidence that the overlapping factors not only serve to ensure the accessibility of a website for all users, but are also useful for the optimization of the website's search engine ranking. The research demonstrates that any SEO project undertaken should include, as a prerequisite, the proper design of accessible web content, inasmuch as search engines will interpret the web accessibility achieved as an indicator of quality and will be able to better access and index the resulting web content.Originality/valueThe present study indicates how developing websites with high visibility in search engine results also makes their content more accessible.
A task‐focused literature recommender system for digital libraries
Authors: Yang Wan‐Shiou , Lin Yi‐Rong 
Published: August 2013

PurposeThe scientific literature has played an important role in the dissemination of new knowledge throughout the past century. However, the increasing numbers of scientific articles being published in recent years has intensified the perception of information overload for users attempting to find relevant scientific information. The purpose of this paper is to describe a task‐focused strategy that employs the task profiles of users to make recommendations in a digital library.Design/method/approachThis paper combines information retrieval, common citation analysis, and coauthor relationship analysis techniques with a citation network analysis technique – the CiteRank algorithm – to find relevant and high‐quality articles. In total, nine variations of the proposed approach were tested using articles downloaded from the CiteSeerX system and usage logs collected from the authors' experimental server.FindingsThe results from the authors' experimental evaluations demonstrate that the proposed Content‐citation approach outperforms the Relevance‐CiteRank, Relevance‐citation count, and Relevance‐only approaches.Originality/valueThis paper describes an original study that has produced a novel way to combine information retrieval, common citation analysis, and coauthor relationship analysis techniques to find relevant and high‐quality articles for recommendation in a digital library.
Kinetic studies of the effect of a 17‐nucleotide difference in the 0.3‐kb region containing the R‐U5‐5′ leader sequence of Friend murine leukemia virus on viral gene expression
Authors: Choo Yeng Cheng , Seki Yohei , Takase‐Yoden Sayaka 
Published: August 2013

ABSTRACTIn addition to the env gene, a 0.3‐kb fragment containing the R‐U5‐5′ leader sequence is essential for the induction of spongiform neurodegeneration by Friend murine leukemia virus (Fr‐MLV) clone A8 and it also influences expression of the Env protein. Kinetic studies were carried out using two recombinant viruses, R7f, carrying the A8 0.3‐kb fragment, and Rec5, carrying the 0.3‐kb fragment of the non‐neuropathogenic Fr‐MLV clone 57. These analyses suggested that the 0.3‐kb fragment influenced the expression level of the Env protein by regulating the amount of spliced env‐mRNA rather than the amount of total viral mRNA or viral production.
Synthesis of ω‐hydroxy dodecanoic acid based on an engineered CYP153A fusion construct
Authors: Scheps Daniel , Honda Malca Sumire , Richter Sven M. , Marisch Karoline , Nestl Bettina M. , Hauer Bernhard 
Published: August 2013

SummaryA bacterial P450 monooxygenase‐based whole cell biocatalyst using Escherichia coli has been applied in the production of ω‐hydroxy dodecanoic acid from dodecanoic acid (C12‐FA) or the corresponding methyl ester. We have constructed and purified a chimeric protein where the fusion of the monooxygenase CYP153A from Marinobacter aquaeloei to the reductase domain of P450 BM3 from Bacillus megaterium ensures optimal protein expression and efficient electron coupling. The chimera was demonstrated to be functional and three times more efficient than other sets of redox components evaluated. The established fusion protein (CYP153AM. aq.‐CPR) was used for the hydroxylation of C12‐FA in in vivo studies. These experiments yielded 1.2 g l–1 ω‐hydroxy dodecanoic from 10 g l–1 C12‐FA with high regioselectivity (> 95%) for the terminal position. As a second strategy, we utilized C12‐FA methyl ester as substrate in a two‐phase system (5:1 aqueous/organic phase) configuration to overcome low substrate solubility and product toxicity by continuous extraction. The biocatalytic system was further improved with the coexpression of an additional outer membrane transport system (AlkL) to increase the substrate transfer into the cell, resulting in the production of 4 g l–1 ω‐hydroxy dodecanoic acid. We further summarized the most important aspects of the whole‐cell process and thereupon discuss the limits of the applied oxygenation reactions referring to hydrogen peroxide, acetate and P450 concentrations that impact the efficiency of the production host negatively.
Is metagenomics resolving identification of functions in microbial communities?
Authors: Chistoserdova Ludmila 
Published: August 2013

SummaryWe are coming up on the tenth anniversary of the broad use of the method involving whole metagenome shotgun sequencing, referred to as metagenomics. The application of this approach has definitely revolutionized microbiology and the related fields, including the realization of the importance of the human microbiome. As such, metagenomics has already provided a novel outlook on the complexity and dynamics of microbial communities that are an important part of the biosphere of the planet. Accumulation of massive amounts of sequence data also caused a surge in the development of bioinformatics tools specially designed to provide pipelines for data analysis and visualization. However, a critical outlook into the field is required to appreciate what could be and what has currently been gained from the massive sequence databases that are being generated with ever‐increasing speed.
SIRT1 but not its increased expression is essential for lifespan extension in caloric‐restricted mice
Authors: Mercken Evi M. , Hu Jia , Krzysik‐Walker Susan , Wei Min , Li Ying , McBurney Michael W. , de Cabo Rafael , Longo Valter D. 
Published: August 2013

SummaryThe SIRT1 deacetylase is one of the best‐studied putative mediators of some of the anti‐aging effects of calorie restriction (CR), but its role in CR‐dependent lifespan extension has not been demonstrated. We previously found that mice lacking both copies of SIRT1 displayed a shorter median lifespan than wild‐type mice on an ad libitum diet. Here, we report that median lifespan extension in CR heterozygote SIRT1+/− mice was identical (51%) to that observed in wild‐type mice, but SIRT1+/− mice displayed a higher frequency of certain pathologies. Although larger studies in additional genetic backgrounds are needed, these results provide strong initial evidence for the requirement of SIRT1 for the lifespan extension effects of CR, but suggest that its high expression is not required for CR‐induced lifespan extension.