bluuebunny/crossref_metadata_2025_split huggingface dataset:
p600 regulates spindle orientation in apical neural progenitors and contributes to neurogenesis in the developing neocortex
Authors: Belzil Camille , Asada Naoyuki , Ishiguro Kei-ichiro , Nakaya Takeo , Parsons Kari , Pendolino Valentina , Neumayer Gernot , Mapelli Marina , Nakatani Yoshihiro , Sanada Kamon , Nguyen Minh Dang 
Published: May 2014

ABSTRACT
Apical neural progenitors (aNPs) drive neurogenesis by means of a program consisting of self-proliferative and neurogenic divisions. The balance between these two manners of division sustains the pool of apical progenitors into late neurogenesis, thereby ensuring their availability to populate the brain with terminal cell types. Using knockout and in utero electroporation mouse models, we report a key role for the microtubule-associated protein 600 (p600) in the regulation of spindle orientation in aNPs, a cellular event that has been associated with cell fate and neurogenesis. We find that p600 interacts directly with the neurogenic protein Ndel1 and that aNPs knockout for p600, depleted of p600 by shRNA or expressing a Ndel1-binding p600 fragment all display randomized spindle orientation. Depletion of p600 by shRNA or expression of the Ndel1-binding p600 fragment also results in a decreased number of Pax6-positive aNPs and an increased number of Tbr2-positive basal progenitors destined to become neurons. These Pax6-positive aNPs display a tilted mitotic spindle. In mice wherein p600 is ablated in progenitors, the production of neurons is significantly impaired and this defect is associated with microcephaly. We propose a working model in which p600 controls spindle orientation in aNPs and discuss its implication for neurogenesis.
The role of DNA damage response in zebrafish and cellular models of Diamond Blackfan Anemia
Authors: Danilova Nadia , Bibikova Elena , Covey Todd M. , Nathanson David , Dimitrova Elizabeth , Konto Yoan , Lindgren Anne , Glader Bertil , Radu Caius G. , Sakamoto Kathleen M. , Lin Shuo 
Published: May 2014

Abstract
Ribosomal biogenesis involves processing of pre-rRNA in assembly with ribosomal proteins (RPs). Deficiency of some RPs impairs processing and causes Diamond Blackfan Anemia (DBA) associated with anemia, congenital malformations, and cancer. p53 mediates many features of DBA but the mechanism of p53 activation remains unclear. Another hallmark of DBA is upregulation of adenosine deaminase (ADA) suggesting changes in nucleotide metabolism. In RP-deficient zebrafish, we found activation of both nucleotide catabolism and biosynthesis consistent with the need to break and replace the faulty rRNA. We also found upregulation of dNTP synthesis, a typical response to replication stress and DNA damage. Both RP-deficient zebrafish and human hematopoietic cells showed activation of the ATR/ATM/CHK1/2/p53 pathway. Other features of RP deficiency included an imbalanced dNTP pool, ATP depletion, and AMPK activation. Replication stress and DNA damage in cultured cells in non-DBA models can be decreased by exogenous nucleosides. Therefore, we treated RP-deficient zebrafish embryos with exogenous nucleosides and observed decreased activation of p53 and AMPK, reduced apoptosis, and rescue of hematopoiesis. Our data suggest that DNA damage response contributes to p53 activation in cellular and zebrafish models of DBA. Furthermore, rescue of RP-deficient zebrafish with exogenous nucleosides suggests that nucleoside supplements may be beneficial in DBA.
Quinoxaline Dendrimers at the Air–Aqueous Interface and Their Photoluminescent Properties
Authors: Choi Soyoung , Eom Yu Kyung , Kim Hwan Kyu , Sohn Daewon 
Published: May 2014

Abstract
The monolayer behaviors of two dendrimers containing a hydrophilic core group (carboxy group) and hydrophobic branches (quinoxaline and methoxyphenyl groups), 2,3-bis{4-[2,3-bis(4-methoxyphenyl)quinoxalin-6-yloxy]phenyl}quinoxaline-6-carboxylic acid (G2) and 2,3-bis[4-(2,3-bis{4-[2,3-bis(4-methoxyphenyl)quinoxalin-6-yloxy]phenyl}quinoxalin-6-yloxy)phenyl]quinoxaline-6-carboxylic acid (G3), were studied using surface pressure–area (Π–A) isotherms and atomic force microscopy (AFM). Surface pressure–area isotherms show that the limiting area of G2 increased but G3 decreased on the europium ion-containing subphase than on the water subphase. The Langmuir–Blodgett (LB) films of dendrimer–europium ion complexes preserve their original luminescent properties and the packing of further layers does not cause quenching of luminescence.
Preparation of Self-stratifying Epoxy–Polyacrylate Emulsions with Three-layer Core–Shell Structures
Authors: Liu Lizhu , Li Jinfeng , Zhang Xiaorui , Jin Ke 
Published: May 2014

Abstract
Epoxy–polyacrylate emulsions with three-layer core–shell structures were synthesized employing a two-stage emulsion polymerization procedure. The films prepared from the emulsions showed signs of self-stratification. This method may be applicable to the study of self-stratifying coatings.
Uptake and Electrochemical Ejection of Cesium Ion by a Prussian Blue-modified Electrode
Authors: Tatsuma Tetsu , Kuroiwa Yoshinori , Ishii Kazuyuki , Kudo Kazuaki , Sakoda Akiyoshi 
Published: May 2014

Abstract
Prussian blue (FeIII4[FeII(CN)6]3) electrochemically deposited on an indium tin oxide electrode was loaded with cesium ion in a diluted aqueous CsCl solution (ca. 40 ppb) followed by reduction or oxidation. Although reduction of Prussian blue does not increase the capacity of cesium uptake, oxidation of Prussian blue releases the entrapped cesium ion.
Synthesis of MCM-41 with High Manganese Content by Mechanochemical Pretreatment of the Starting Materials
Authors: Iida Takayuki , Sato Mitsutaka , Liu Zhendong , Numako Chiya , Nakahira Atsushi , Okubo Tatsuya , Wakihara Toru 
Published: May 2014

Abstract
Manganese-incorporated MCM-41 with high manganese content of Mn/(Mn + Si) = 9.2 mol % and BET specific surface area of 750 m2 g−1 was obtained by utilizing mechanochemical treatment. Clear differences in the state of manganese throughout the synthetic conditions were confirmed by various spectroscopic analyses.
Eclipsed Columnar Packing in Crystal Structure of Sumanenetrione
Authors: Shrestha Binod Babu , Morita Yuki , Kojima Tatsuhiro , Kawano Masaki , Higashibayashi Shuhei , Sakurai Hidehiro 
Published: May 2014

Abstract
Single crystals of sumanenetrione and the iodinated derivative were found to possess a columnar packing structure with characteristic eclipsed stacks in a convex-to-concave fashion. In each column, the molecules are perfectly stacked without twisting and slipping in contrast to pristine sumanene. The neighboring columns are arranged in the opposite directions.
Highly Efficient Water Oxidation on Rutile Titanium(IV) Oxide Powder in the Presence of Iodate Ions as Reversible Electron Acceptors
Authors: Maeda Kazuhiko 
Published: May 2014

Abstract
A high apparent quantum yield (AQY) of 25 ± 1% (at 365 nm) for water oxidation in the presence of iodate ions was achieved using a rutile titanium(IV) oxide that has relatively low density of surface defects.
Mark Twain’s satanic existentialist: The Mysterious Stranger
Authors: Kravitz Bennett 
Published: May 2014

Abstract
This article attempts to situate Mark Twain’s The Mysterious Stranger in the context of American optimism at the onset of the twentieth century. While often considered one of the most pessimistic of Twain’s novels, The Mysterious Stranger is portrayed as Twain’s engagement with the philosophy of existentialism. Specifically, the article examines the ways that the text engages the existential premises of Jean Paul Sartre. Rather than abandoning his American optimism, Twain essentially destroys the visible world of endless warfare and religious obstruction to allow Americans to reclaim their Emersonian optimism by suggesting that we all ‘Dream other dreams and better.’ This article argues that the margins of the text suggest an existential attempt to come to terms with the disappointments of the Gilded Age and the inconsistencies of early twentieth-century America and its capitalistic society.
The hospitalities of cities: Between the agora and the fortress
Authors: Broek Chávez Frans van den , van der Rest Jean-Pierre 
Published: May 2014

Abstract
This article calls attention to the inherent complexities of analysing and understanding the hospitalities of cities. Drawing on an urban sociological view of the city it proposes a metaphor – the agora and the fortress – as two extreme opposites, to identify levels, places and forms it can comprise. Utilizing the ‘dynamic model of hospitality’ as an analytical framework, it explores and exemplifies several host–guest relationships from a city government perspective and their dynamic interactions with higher levels of agency. These interactions are discussed in the context of cosmopolitanism, urbanism and public policy. The article concludes that the hospitalities of cities involve a variety of human phenomena that cannot be defined without ambiguity, but which are essential to our understanding of the flourishing and growth of cities, and their hospitalities. Future research should therefore include many lines of enquiry and research that no one single discipline can pursue.
Reviews
Authors: Young Elizabeth Drummond , Robinson Richard N.S. , Crowther Alicia 
Published: May 2014

Abstract
Hospitality as Holiness: Christian Witness Amid Moral Diversity, Luke Bretherton (2010) Farnham: Ashgate, 226 pp., ISBN: 9781409403494, p/bk, £19.99
The Limits of Hospitality, Jessica Wrobleski (2012) Collegeville, MN: Liturgical Press, 168 pp., ISBN: 9780814657645, p/bk, $19.95
Eating Together: Food, Friendship and Inequality, Alice P. Julier (2013) Urbana, IL: University of Illinois Press, ix + 237 pp., ISBN: 9780252037634, p/bk, £16.99
Espectros, trauma e historia: La ‘hauntología’ de Guillermo del Toro en El espinazo del diablo
Authors: Rodero Jesús 
Published: May 2014

Abstract
En El espinazo del diablo (2001) el director mexicano Guillermo del Toro ofrece una re-visión/re-escritura en clave sobrenatural de la guerra civil española. Ha habido entre la crítica visiones encontradas sobre la significación de la inserción de lo sobrenatural en el contexto histórico concreto de la guerra civil española. Mientras parte de la crítica hace hincapié en el supuesto carácter finalista y revisionista de este film por su re-interpretación y re-localización de la historia a través de una brillante yuxtaposición de lo irreal y lo real que refuerza la denuncia de los horrores y traumas de la historia (Hardcastle); otra parte resaltan las contradicciones que parecen definir a los personajes para hacer una interpretación ‘hauntológica’ de la película (Brinks, Lázaro-Reboll).
Este trabajo explora la interrelación entre las dos estructuras de El espinazo del diablo, la histórico-referencial y la sobrenatural, para ver hasta qué punto las convenciones del género del horror refuerzan o diluyen la dimensión histórica del film y hasta qué punto el ‘mensaje político’ se puede considerar una recreación catártica de la historia o un cuestionamiento sin resolución final sobre los traumas del pasado. Para ello, nos apoyaremos fundamentalmente en la teoría ‘hauntológica’ de Jacques Derrida y en otros estudios sobre lo fantástico y espectral más recientes (algunos mencionados arriba) que han aplicado las teorías derridianas al cine de la guerra civil.
Activation of the Pleiotropic Drug Resistance Pathway Can Promote Mitochondrial DNA Retention by Fusion-Defective Mitochondria in Saccharomyces cerevisiae
Authors: Mutlu Nebibe , Garipler Görkem , Akdoğan Emel , Dunn Cory D 
Published: May 2014

Abstract
Genetic and microscopic approaches using Saccharomyces cerevisiae have identified many proteins that play a role in mitochondrial dynamics, but it is possible that other proteins and pathways that play a role in mitochondrial division and fusion remain to be discovered. Mutants lacking mitochondrial fusion are characterized by rapid loss of mitochondrial DNA. We took advantage of a petite-negative mutant that is unable to survive mitochondrial DNA loss to select for mutations that allow cells with fusion-deficient mitochondria to maintain the mitochondrial genome on fermentable medium. Next-generation sequencing revealed that all identified suppressor mutations not associated with known mitochondrial division components were localized to PDR1 or PDR3, which encode transcription factors promoting drug resistance. Further studies revealed that at least one, if not all, of these suppressor mutations dominantly increases resistance to known substrates of the pleiotropic drug resistance pathway. Interestingly, hyperactivation of this pathway did not significantly affect mitochondrial shape, suggesting that mitochondrial division was not greatly affected. Our results reveal an intriguing genetic connection between pleiotropic drug resistance and mitochondrial dynamics.
Detecting Local Haplotype Sharing and Haplotype Association
Authors: Xu Hanli , Guan Yongtao 
Published: May 2014

Abstract
A novel haplotype association method is presented, and its power is demonstrated. Relying on a statistical model for linkage disequilibrium (LD), the method first infers ancestral haplotypes and their loadings at each marker for each individual. The loadings are then used to quantify local haplotype sharing between individuals at each marker. A statistical model was developed to link the local haplotype sharing and phenotypes to test for association. We devised a novel method to fit the LD model, reducing the complexity from putatively quadratic to linear (in the number of ancestral haplotypes). Therefore, the LD model can be fitted to all study samples simultaneously, and, consequently, our method is applicable to big data sets. Compared to existing haplotype association methods, our method integrated out phase uncertainty, avoided arbitrariness in specifying haplotypes, and had the same number of tests as the single-SNP analysis. We applied our method to data from the Wellcome Trust Case Control Consortium and discovered eight novel associations between seven gene regions and five disease phenotypes. Among these, GRIK4, which encodes a protein that belongs to the glutamate-gated ionic channel family, is strongly associated with both coronary artery disease and rheumatoid arthritis. A software package implementing methods described in this article is freely available at http://www.haplotype.org.
Neutron Scattering of CeNi at the SNS-ORNL: A Preliminary Report
Authors: Mirmelstein A.V. , Podlesnyak A. , Kolesnikov A.I. , Saporov B. , Sefat A.S. , Tobin JG 
Published: May 2014

ABSTRACTThis is a preliminary report of a neutron scattering experiment used to investigate 4f electron behavior in Ce.
Effects of Light Activated In-office Bleaching on Permeability, Microhardness, and Mineral Content of Enamel
Authors: Parreiras SO , Vianna P , Kossatz S , Loguercio AD , Reis A 
Published: May 2014

SUMMARY
The aim of this study was to evaluate the permeability (PE), microhardness (KHN), and mineral change in enamel after LED/laser activated in-office bleaching. For PE, the coronal portion of premolars (n=51) was subjected to bleaching with 35% hydrogen peroxide (Whiteness HP Maxx, FGM Dental Products, Joinville, SC, Brazil). The samples were stained via the histochemical method, which involves a copper sulphate solution and rubeanic acid. The penetration of dye into the enamel was measured. The KHN of enamel was assessed before treatment, immediately after the bleaching treatment, and again after one week. The calcium and phosphorus content were analyzed with a scanning electron microscope with energy-dispersive X-ray (JSM 6360LV, Jeol Ltd, Tokyo, Japan). The data set from each test was subjected to appropriate parametric statistical analysis (α=0.05). No significant differences were observed for PE in NLA and LA compared to the control group (p=0.98), as well as for calcium (p=0.16) and phosphorus (p=0.80) content. Significant reduction of KHN after bleaching occurred for both groups (p&lt;0.001). After immersion in artificial saliva, the KHN of the enamel for all groups was similar to that seen before bleaching. Light activation during in-office bleaching does not produce significant changes in the enamel compared to a non–light-activated technique.
Anti‐proliferative and anti‐tumour effects of lymphocyte‐derived microparticles are neither species‐ nor tumour‐type specific
Authors: Yang Chun , Xiong Wei , Qiu Qian , Tahiri Houda , Superstein Rosanne , Carret Anne‐Sophie , Sapieha Przemyslaw , Hardy Pierre 
Published: May 2014

BackgroundUnregulated cell proliferation or growth is a prominent characteristic of cancer. We have previously demonstrated that LMPs (cell membrane microparticles derived from apoptotic human CEM T lymphoma cells stimulated with actinomycin D) strongly suppress the proliferation of not only human endothelial cells but also mouse Lewis lung carcinoma cells.MethodsLMPs were generated either from CEM T cells using different stimuli or from 3 different types of lymphocytes. The effects of LMPs on cancer cell proliferation were examined using cell lines from different species and tissues. The cell cycle kinetics was evaluated by FACS and the expression of cell cycle‐related genes was determined using quantitative RT‐PCR. The in vivo anti‐tumor effect of LMPs was investigated using xenografts and allografts.ResultsLMPs at doses far above physiological levels dramatically suppressed the proliferation of cancer cells in a non species‐specific manner. LMPs selectively target high proliferating cells and their anti‐proliferative effect is not dependent on parental cell origin or stimuli. The anti‐proliferative effect of LMPs was due to induction of cell‐cycle arrest in G0/G1, with associated increases in expression of the cyclin‐dependent kinase inhibitors p15INK4b, p16INK4a, and p21Cip1. In vivo, LMPs significantly suppressed tumor growth in animal tumor models.ConclusionThese results highlight the potential role of LMPs in modulating the growth of high proliferating cells. Given that cell‐based therapies are considered less toxic than pharmacologic approaches and have the potential to target multiple pathways in a synergistic manner, LMPs may serve as a veritable option for cancer treatment.
Flower morphology and floral sequence in Artemisia annua (Asteraceae)1
Authors: Wetzstein Hazel Y. , Porter Justin A. , Janick Jules , Ferreira Jorge F. S. 
Published: May 2014

• Premise of the study: Artemisia annua produces phytochemicals possessing antimalarial, antitumor, anti‐inflammatory, and anthelmintic activities. The main active ingredient, artemisinin, is extremely effective against malaria. Breeding to develop cultivars producing high levels of artemisinin can help meet worldwide demand for artemisinin and its derivatives. However, fundamental reproductive processes, such as the sequence of flowering and fertility, are not well understood and impair breeding and seed propagation programs.• Methods: Capitulum structure and floral sequence were studied using light and scanning electron microscopy to describe inflorescence architecture, floret opening, and seed set.• Key results: Florets are minute and born in capitula containing pistillate ray florets and hermaphroditic disk florets. Ray florets have elongated stigmatic arms that extend prior to disk floret opening. Disk florets exhibit protandry. During the staminate phase, pollen is released within a staminate tube and actively presented with projections at the tip of stigmas as the pistil elongates. During the pistillate phase, stigmatic arms bifurcate and reflex. Stigmas are of the dry type and stain positively for polysaccharides, lipids, and an intact cuticle. Floret numbers vary with genotype, and capitula are predominantly composed of disk florets. Both ray and disk florets produce filled seed.• Conclusions: Gynomonoecy, early opening of ray florets, and dichogamy of disk florets promote outcrossing in A. annua. For breeding and seed development, flowering in genotypes can be synchronized under short days according to the floral developmental stages defined. Floret number and percentage seed fill vary with genotype and may be a beneficial selection criterion.
The evolution of bark mechanics and storage across habitats in a clade of tropical trees
Authors: Rosell Julieta A. , Olson Mark E. 
Published: May 2014

•Premise of the study:Bark functional strategies vary conspicuously within communities. As a result, predicting most community‐level bark traits based on environment often reveals little association. To complement this community‐based view, we took a clade‐based approach to study potentially adaptive differences in bark water storage and biomechanics across habitats and examined ontogenetic mechanisms that lead to these differences.•Methods:We studied the branches of nine species in thesimarubaclade ofBurserain dry to wet, fire‐free neotropical forests. We measured mechanical properties from branch tips to bases, as well as the relative area and water content of bark. Using raw data and phylogenetically independent contrasts, we then tested predictions regarding trait associations with environment and mapped branch tip‐to‐base ontogenetic changes.•Key results:Across our wet–dry gradient, bark water storage was greater in drier habitats, whereas bark tissue mechanical rigidity was greater in the taller species of moister forests. Bark was the principal mechanical tissue in branch tips and an important contributor even in branches 3 m long. Within species, bark contributions to mechanical support and water storage came mostly through a tip‐to‐base increase in bark quantity rather than alterations in tissue properties. Quantitative developmental alterations in proportions of bark to wood led to habit differences.•Conclusions:Our clade‐based approach shows that, in marked contrast to most community‐based results, environment can strongly predict bark functional traits across species in ways that seem plausibly adaptive.
Determination of the Synchronous Machine's Parameters Using the iLab Shared Architecture
Authors: Naddami Ahmed , Fahli Ahmed , Gourmaj Mourad , Moussetad Mohammed 
Published: May 2014

The target of this work is to promote the experimental works via internet within the different laboratories of Hassan 1st University in Settat and our partners in eSiense group within the frame of Tempus project.
Tempus project aims to create a series of remote laboratories in Morocco, Tunisia and Algeria in cooperation with France, Austria, Romania and Greece and it is financed by the European Union.
In order to share knowledge, Morocco and these universities are working together in a collective work program in order to create leading laboratories, aiming to put into practice the experimental works in electrical engineering, that are accessible through internet. These experimental works tend to share the equipments existing in every University so as to make them possible for other universities to benefit from them.
This leading project will be generalized afterwards in order to engulf all the other fields, including different manipulations of measuring powers using electromechanical machines and measures concerning instruments.
This paper focuses on the implementation of the first remote laboratory in Hassan 1st University in morocco. It concerns the determination of the characteristics of the synchronous machine. For this, student accesses to our platform via internet to determine the parameters in Open Circuit characteristics OCC, Short circuit characteristics SCC and then the determination of the synchronous reactance Xs.