bluuebunny/crossref_metadata_2025_split huggingface dataset:
GRK3 is essential for metastatic cells and promotes prostate tumor progression
Authors: Li Wenliang , Ai Nanping , Wang Suming , Bhattacharya Nandita , Vrbanac Vladimir , Collins Michael , Signoretti Sabina , Hu Yanhui , Boyce Frederick M. , Gravdal Karsten , Halvorsen Ole J. , Nalwoga Hawa , Akslen Lars A. , Harlow Ed , Watnick Randolph S. 
Published: January 2014

Significance
Although the majority of cancer-related deaths are consequences of metastatic dissemination, the molecular and cellular forces that drive tumor cell dispersion are still poorly understood. To help identify new regulators that play critical roles in these processes, we screened for human kinases that are important for continued survival of metastatic cells. One kinase identified from these screens, the G-protein–coupled receptor kinase 3 (GRK3; or β-adrenergic receptor kinase 2), was found to have a key role in promoting prostate tumor growth and metastasis in mouse models through enhancing angiogenesis. Notably, GRK3 is overexpressed in human prostate metastatic tumors. Further studies on GRK3 and its pathways promise to expand our knowledge of cancer metastasis and also yield new cancer therapeutic targets.
Increasing socioeconomic disparities in adolescent obesity
Authors: Frederick Carl B. , Snellman Kaisa , Putnam Robert D. 
Published: January 2014

Significance
Childhood and youth obesity represent significant US public health challenges. Recent findings that the childhood obesity ‘‘epidemic’’ may have slightly abated have been met with relief from health professionals and popular media. However, we document that the overall trend in youth obesity rates masks a significant and growing class gap between youth from upper and lower socioeconomic status (SES) backgrounds. Until 2002, obesity rates increased at similar rates for all adolescents, but since then, obesity has begun to decline among higher SES youth but continued to increase among lower SES youth. These results underscore the need to target public health interventions to disadvantaged youth who remain at risk, as well as to examine how health information circulates through class-biased channels.
Living liquid crystals
Authors: Zhou Shuang , Sokolov Andrey , Lavrentovich Oleg D. , Aranson Igor S. 
Published: January 2014

SignificanceWe propose a class of active matter, the living liquid crystal (LLC), representing motile rod-shaped bacteria placed in water-based nontoxic liquid crystal. Long-range orientational order of the liquid crystal and the swimming activity of bacteria demonstrate a strong coupling that dramatically alters individual and collective bacterial dynamics. For example, swimming bacteria perturb the orientational order of the liquid crystal or even cause its local melting, making the flagella motion optically visible. Second, self-organized textures emerge from the initial uniform LLC alignment with a characteristic length controlled by a balance between bacteria activity and anisotropic viscoelasticity of liquid crystal. Third, the local liquid crystal orientation controls the direction of motion of bacteria. LLC can lead to valuable biosensoring and biomedical applications.
Timing of cyber conflict
Authors: Axelrod Robert , Iliev Rumen 
Published: January 2014

Significance
The world’s economy and international security have come to depend upon a secure Internet. International rivalries and conflicts have already provided challenges to Internet security in the form of espionage, sabotage, and denial of service. New vulnerabilities in computer systems are constantly being discovered. When an individual, group, or nation has access to means of exploiting such vulnerabilities in a rival’s computer systems, it faces a decision of whether to exploit its capacity immediately or wait for a more propitious time. This paper introduces a simple mathematical model applied to four case studies to promote the understanding of the new domain of cyber conflict.
Integrated description of protein dynamics from room-temperature X-ray crystallography and NMR
Authors: Fenwick R. Bryn , van den Bedem Henry , Fraser James S. , Wright Peter E. 
Published: January 2014

Significance
Most proteins are inherently flexible and their dynamics play a central role in their biological functions. A molecular level understanding of protein function and mechanism requires an accurate description of the atomic coordinates in both time and space. Here we show, through studies of the enzyme dihydrofolate reductase, that multiconformer models derived from room-temperature X-ray crystallographic data can be used synergistically with nuclear magnetic resonance relaxation measurements to provide a detailed description of both the amplitude and timescale of fluctuations in atomic coordinates. This hybrid approach provides a more complete description of protein dynamics than can be obtained from either method alone. The room-temperature crystallographic ensemble accurately reflects the picosecond–nanosecond motions of the protein backbone and side chains.
The WNT receptor FZD7 is required for maintenance of the pluripotent state in human embryonic stem cells
Authors: Fernandez Antonio , Huggins Ian J. , Perna Luca , Brafman David , Lu Desheng , Yao Shiyin , Gaasterland Terry , Carson Dennis A. , Willert Karl 
Published: January 2014

SignificanceEmbryonic stem cells (ESCs) are unique in their ability to expand and self-renew indefinitely while retaining the potential to give rise to all mature cell types. The molecular mechanisms underlying these properties remain poorly understood. We investigated the role of the highly conserved WNT signaling pathway in controlling self-renewal and found that the WNT receptor encoded by the frizzled family receptor 7 (FZD7) gene is essential for maintaining human ESCs in an undifferentiated and pluripotent state. Using an FZD7-specific fragment antigen binding protein, as well as knockdown of FZD7 expression, we showed that the FZD7 receptor transduces a WNT/β-catenin signal in human ESCs. These data demonstrate that an endogenous WNT signaling loop is essential for the maintenance of human ESCs in an undifferentiated state.
Rapid Prediction of Rice Quality Characteristics by Near‐Infrared Reflectance Spectroscopy for Breeding Programs
Authors: Sun Chengxiao , Yu Yonghong , Duan Bingwu , Zhu Zhiwei 
Published: January 2014

ABSTRACTBreeding of high‐quality rice requires quick methods to evaluate the quality characteristics such as milling, grain appearance, nutritional, eating, and cooking qualities. Because routine measurements of these quality traits are time consuming and expensive, a rapid predictive method based on near‐infrared spectroscopy (NIRS) can be applied to measure these quality parameters. In this study, calibration models for measurement of grain quality were developed using a total of 570 brown and milled rice samples. The results indicated that the models developed from the spectra of brown rice for all the quality traits had the coefficient of determination for external validation (R2) larger than 0.64 except for gel consistency. The best model was developed for the protein content, with R2 of 0.94 for external validation. The model for the total score of physicochemical characteristics (TSPC), a comprehensive index reflecting all other traits, had R2 of 0.70 and SD/SEP of 1.70, which indicates that high or low TSPC for a given rice could be discriminated by NIRS. The models developed from brown rice were as accurate as those from milled rice. Results suggest that NIRS‐based predictions for rice quality traits may be used as indicator traits to improve rice quality in breeding programs.
Effects of Genotype and Environment on Phenolic Acids Content and Total Antioxidant Capacity in Durum Wheat
Authors: Martini Daniela , Taddei Federica , Nicoletti Isabella , Ciccoritti Roberto , Corradini Danilo , D'Egidio Maria Grazia 
Published: January 2014

ABSTRACTIn cereals, phenolic acid (PA) content and total antioxidant capacity (TAC) may have a wide range of variability, probably because of several factors influencing the occurrence of grain antioxidants, which include genotype, environment, and their possible interactions. However, only a few studies have investigated the influence of these factors on durum wheat. In the present study, we investigated the impact of the genetic and environmental factors on the profile and content of PAs occurring as soluble free, soluble conjugated, and insoluble bound compounds, as well as on the TAC level, in three genotypes of durum wheat grown in three different Italian agroclimatic areas during two crop years. The results show that genotype, environment, and crop year have highly significant effects on TAC levels and on PA contents. In particular, TAC and free PAs are most influenced by year, whereas conjugated and bound PAs are most influenced by environment × year and genotype, respectively. Therefore, it is evidenced that genetic and environmental factors affect the antioxidant activity and the content of the three forms of PAs in durum wheat to different extents.
Role Reversal Method for Treatment of Food Refusal Associated With Infantile Feeding Disorders
Authors: Segal Idit , Tirosh Anat , Sinai Tali , Alony Sari , Levi Anat , Korenfeld Lia , Zangen Tsili , Mizrachi Avi , Boaz Mona , Levine Arie 
Published: January 2014

ABSTRACTObjectives:Infantile feeding disorders (IFDs) are common causes of food refusal and failure to thrive, and are frequently encountered by primary care physicians and specialists. We have published the Wolfson criteria for IFD, which have eased the approach to the diagnosis of IFDs. Along with and complementary to the Wolfson criteria, we have also developed the role reversal treatment method for IFD, which has been briefly described earlier. The aim of this study was to validate the role reversal treatment method on a cohort of infants diagnosed as having IFD and to present a detailed description of this method for the first time.Methods:Parents of infants and children diagnosed as having IFD were invited to participate in the study; they were handed over a questionnaire comprising 6 categories of questions related to patient and parents behaviors, attitudes, and perceptions, which was completed at initiation and at the end of treatment. Full response was defined as improved normative feeding, cessation of abnormal parental feeding, and improved or normal growth patterns. A partial response was defined as success with two‐third categories.Results:We enrolled 38 patients, and 32 patients completed the study. Improved feeding occurred in 78%, full recovery was documented in 53% of infants by 6 months, and partial response was observed in another 25%. All forms of pathological feeding improved significantly (mechanistic, nocturnal, persecutory, forced feeding, and distraction).Conclusions:The role reversal treatment method is a simple and effective approach to the treatment of food refusal associated with IFD.
Trithorax Group Protein Oryza sativa Trithorax1 Controls Flowering Time in Rice via Interaction with Early heading date3
Authors: Choi Sang Chul , Lee Shinyoung , Kim Sung-Ryul , Lee Yang-Seok , Liu Chunyan , Cao Xiaofeng , An Gynheung 
Published: January 2014

Abstract
Trithorax group proteins are chromatin-remodeling factors that activate target gene expression by antagonistically functioning against the Polycomb group. In Arabidopsis (Arabidopsis thaliana), Arabidopsis Trithorax protein1 (ATX1) regulates flowering time and floral organ identity. Here, we observed that suppression of Oryza sativa Trithorax1 (OsTrx1), an ortholog of ATX1, delayed flowering time in rice (Oryza sativa). Because the delay occurred only under long-day conditions, we evaluated the flowering signal pathways that specifically function under long-day conditions. Among them, the OsMADS50 and Heading date1 pathways were not affected by the mutation. However, the Grain number, plant height, and heading date7 (Ghd7) pathway was altered in ostrx1. Transcript levels of OsGI, phytochrome genes, and Early heading date3 (Ehd3), which function upstream of Ghd7, were unchanged in the mutant. Because Trx group proteins form a complex with other proteins to modify the chromatin structure of target genes, we investigated whether OsTrx1 interacts with a previously identified protein that functions upstream of Ghd7. We demonstrated that the plant homeodomain motif of OsTrx1 binds to native histone H3 from the calf thymus and that OsTrx1 binds to Ehd3 through the region between the plant homeodomain and SET domains. Finally, we showed that the SET domain at the C-terminal end of OsTrx1 has histone H3 methyltransferase activity when incubated with oligonucleosomes. Our results suggest that OsTrx1 plays an important role in regulating flowering time in rice by modulating chromatin structure.
Appraisal of a new 1D weighted joint inversion of ground based and helicopter‐borne electromagnetic data
Authors: Sudha  , Tezkan Bülent , Siemon Bernhard 
Published: January 2014

ABSTRACTIn order to couple spatial data from frequency‐domain helicopter‐borne electromagnetics with electromagnetic measurements from ground geophysics (transient electromagnetics and radiomagnetotellurics), a common 1D weighted joint inversion algorithm for helicopter‐borne electromagnetics, transient electromagnetics and radiomagnetotellurics data has been developed. The depth of investigation of helicopter‐borne electromagnetics data is rather limited compared to time‐domain electromagnetics sounding methods on the ground. In order to improve the accuracy of model parameters of shallow depth as well as of greater depth, the helicopter‐borne electromagnetics, transient electromagnetics, and radiomagnetotellurics measurements can be combined by using a joint inversion methodology. The 1D joint inversion algorithm is tested for synthetic data of helicopter‐borne electromagnetics, transient electromagnetics and radiomagnetotellurics. The proposed concept of the joint inversion takes advantage of each method, thus providing the capability to resolve near surface (radiomagnetotellurics) and deeper electrical conductivity structures (transient electromagnetics) in combination with valuable spatial information (helicopter‐borne electromagnetics). Furthermore, the joint inversion has been applied on the field data (helicopter‐borne electromagnetics and transient electromagnetics) measured in the Cuxhaven area, Germany.In order to avoid the lessening of the resolution capacities of one data type, and thus balancing the use of inherent and ideally complementary information content, a parameter reweighting scheme that is based on the exploration depth ranges of the specific methods is proposed. A comparison of the conventional joint inversion algorithm, proposed by Jupp and Vozoff (), and of the newly developed algorithm is presented. The new algorithm employs the weighting on different model parameters differently. It is inferred from the synthetic and field data examples that the weighted joint inversion is more successful in explaining the subsurface than the classical joint inversion approach. In addition to this, the data fittings in weighted joint inversion are also improved.
Still a ‘Force for Good’? Good International Citizenship in British Foreign and Security Policy
Authors: Gilmore Jonathan 
Published: January 2014

Research Highlights and Abstract Through an analysis of recent foreign policy discourse, in particular the UK approach to stabilisation, the article identifies distinctive similarities and continuities in the patterns of ethically-minded foreign policy, employed by the 1997–2010 Labour government and the Conservative-Liberal Democrat Coalition. The article makes a fresh engagement with the concept of Good International Citizenship, arguing for a much more overt engagement, by policymakers, with the concept, to help address some of the shortcomings associated with both Labour and the Coalition's attempts to incorporate ethical obligations into British foreign policy. This article examines the interaction between the idea of Good International Citizenship and the recent evolution of UK foreign and security policy. Good International Citizenship centres on the mediation of ethical commitments to national interest, to the promotion international order, and to the wellbeing of vulnerable non-citizen populations. Whilst ethical commitments to non-citizens have become overt components of British foreign policy, recent UK governments have struggled to reconcile these with commitments to the national interest and a stable international order. The article argues that the more direct affirmation of Good International Citizenship as a narrative and ethos of practice for UK foreign policy might help further the increasingly open discussion on the ethics and UK foreign policy. As a framework for dialogue and public debate, the concept provides a means by which ethical commitments in the traditionally closed world of foreign policy might be opened up to wider critical scrutiny.
Effects of solvent fractions of Allomyrina dichotoma larvae through the inhibition of in vitro BACE1 and β‐amyloid(25–35)‐induced toxicity in rat pheochromocytoma PC12 cells
Authors: Kim Minji , Youn Kumju , Yun Eun‐Young , Hwang Jae‐Sam , Ahn Mok‐Ryeon , Jeong Woo‐Sik , Jun Mira 
Published: January 2014

AbstractAmyloid‐β peptide (Aβ) generation initiated by β‐site amyloid precursor protein cleaving enzyme 1 BACE1 is a critical cause of Alzheimer's disease. In the course of our ongoing investigation of natural anti‐dementia resources, the ethyl acetate (EtOAc) fraction exerted strong BACE1‐specific inhibition with the half maximal inhibitory concentration (IC50) value of 9.2 × 10−5 μg/mL. Furthermore, Aβ(25–35)‐induced cell death was predominantly prevented by the EtOAc fraction of Allomyrina dichotoma larvae through diminishing of cellular oxidative stress and attenuating apoptosis by inhibiting caspase‐3 activity. Taken together, the present study demonstrated that A. dichotoma larvae possess novel neuroprotective properties not only via the selective and specific inhibition of BACE1 activity but also through the alleviation of Aβ(25–35)‐induced toxicity, which may raise the possibility of therapeutic application of A. dichotoma larvae for preventing and/or treating dementia.
Difference in apical and basal growth of the frontal bone primordium in Foxc1ch/ch mice
Authors: Machida Akihiko , Okuhara Shigeru , Harada Kiyoshi , Iseki Sachiko 
Published: January 2014

AbstractThe frontal and parietal bones form the major part of the calvarium and their primordia appear at the basolateral region of the head and grow apically. A spontaneous loss of Foxc1 function mutant mouse, congenital hydrocephalus (Foxc1ch/ch), results in congenital hydrocephalus accompanied by defects in the apical part of the skull vault. We found that during the initiation stage of apical growth of the frontal bone primordium in the Foxc1ch/ch mouse, the Runx2 expression domain extended only to the basal side and bone sialoprotein (Bsp) and N‐cadherin expression domains appeared only in the basal region. Fluorescent dye (DiI) labeling of the frontal primordium by ex‐utero surgery confirmed that apical extension of the frontal bone primordium of the mouse was severely retarded, while extension to the basal side underneath the brain was largely unaffected. Consistent with this observation, decreased cell proliferation activity was seen at the apical tip but not the basal tip of the frontal bone primordium as determined by double detection of Runx2 transcripts and BrdU incorporation. Furthermore, expression of the osteogenic‐related genes Bmp4 and‐7 was observed only in the basal part of the meninges during the initiation period of primordium growth. These results suggest that a loss of Foxc1 function affects skull bone formation of the apical region and that Bmp expression in the meninges might influence the growth of the calvarial bone primordium.
Voting Systems for Environmental Decisions
Authors: BURGMAN MARK A. , REGAN HELEN M. , MAGUIRE LYNN A. , COLYVAN MARK , JUSTUS JAMES , MARTIN TARA G. , ROTHLEY KRIS 
Published: January 2014

AbstractVoting systems aggregate preferences efficiently and are often used for deciding conservation priorities. Desirable characteristics of voting systems include transitivity, completeness, and Pareto optimality, among others. Voting systems that are common and potentially useful for environmental decision making include simple majority, approval, and preferential voting. Unfortunately, no voting system can guarantee an outcome, while also satisfying a range of very reasonable performance criteria. Furthermore, voting methods may be manipulated by decision makers and strategic voters if they have knowledge of the voting patterns and alliances of others in the voting populations. The difficult properties of voting systems arise in routine decision making when there are multiple criteria and management alternatives. Because each method has flaws, we do not endorse one method. Instead, we urge organizers to be transparent about the properties of proposed voting systems and to offer participants the opportunity to approve the voting system as part of the ground rules for operation of a group.Sistemas de Votación para Decisiones Ambientales
Occupancy Patterns of Regionally Declining Grassland Sparrow Populations in a Forested Pennsylvania Landscape
Authors: HILL JASON M. , DIEFENBACH DUANE R. 
Published: January 2014

AbstractOrganisms can be affected by processes in the surrounding landscape outside the boundary of habitat areas and by local vegetation characteristics. There is substantial interest in understanding how these processes affect populations of grassland birds, which have experienced substantial population declines. Much of our knowledge regarding patterns of occupancy and density stem from prairie systems, whereas relatively little is known regarding how occurrence and abundance of grassland birds vary in reclaimed surface mine grasslands. Using distance sampling and single‐season occupancy models, we investigated how the occupancy probability of Grasshopper (Ammodramus savannarum) and Henslow's Sparrows (A. henslowii) on 61 surface mine grasslands (1591 ha) in Pennsylvania changed from 2002 through 2011 in response to landscape, grassland, and local vegetation characteristics . A subset (n = 23; 784 ha) of those grasslands were surveyed in 2002, and we estimated changes in sparrow density and vegetation across 10 years. Grasshopper and Henslow's Sparrow populations declined 72% and 49%, respectively from 2002 to 2011, whereas overall woody vegetation density increased 2.6 fold. Henslow's Sparrows avoided grasslands with perimeter–area ratios ≥0.141 km/ha and woody shrub densities ≥0.04 shrubs/m2. Both species occupied grasslands ≤13 ha, but occupancy probability declined with increasing grassland perimeter–area ratio and woody shrub density. Grassland size, proximity to nearest neighboring grassland (= 0.2 km), and surrounding landscape composition at 0.5, 1.5, and 3.0 km were not parsimonious predictors of occupancy probability for either species. Our results suggest that reclaimed surface mine grasslands, without management intervention, are ephemeral habitats for Grasshopper and Henslow's Sparrows. Given the forecasted decline in surface coal production for Pennsylvania, it is likely that both species will continue to decline in our study region for the foreseeable future.Patrones de Ocupación de Poblaciones Regionalmente Declinantes de Gorriones de Pastizales en un Paisaje Boscoso de Pennsylvania