bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of COMBOzyme<sup>®</sup> and Renasol AD<sub>3</sub>E<sup>®</sup> on Body Weight and Hematological Parameters in Broiler Chicken
Authors: Mustari A , Rana S , Rahman MM 
Published: September 2013

The present research work was undertaken to study the effect of multi enzymes and vitamin AD3E on body weight and hematological parameter (TEC, Hb Concentration and ESR) in growing broilers fed with enzyme and vitamin AD3E with normal broiler ration. 20 broiler chickens of 10 (ten) days old Hubbard Classic were taken for study. The chicks were divided randomly into four groups with five chicks in each group. Group A designated as control group and was given normal broiler ration and rest of the groups were given enzyme and vitamin preparation. Group B was given enzyme only, group C was given vitamin only and group D was given both enzyme and vitamin as per schedule. It was observed that enzyme and vitamin AD3E preparation enhanced the growth rate of broilers. On the final day of experiment the body weight was significantly increased (P<0.01) in the treated groups in comparison to that of control group, TEC, Hb concentration and ESR values were significantly (P<0.01) increased in all treated groups than the control group and among the treated groups vitamin AD3E showed lowest performance.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16471 Progress. Agric. 22(1 & 2): 97-103, 2011
Cryopreservation of Sperm of <i>Labeo rohita</i> (Hamilton, 1822) and Its Use in Fertilization and Hatching of Eggs
Authors: Sarder MRI , Rahman AKMA , Samad MS , Nazrul KMS , Bhuiyan MKJ 
Published: September 2013

Cryopreservation is one of the widely accepted and useful methods for preservation of gamete especially the spermatozoa. Experiments were conducted to develop the protocols for cryopreservation of sperm of Labeo rohita and to assess the effect of cryopreserved sperm on fertilization and hatching. Six extenders Alsevers solution, egg-yolk citrate, urea egg-yolk, Kurokura-2, Ma and Mb and five cryoprotectants ethanol, methanol, DMSO, DMA and glycerol were employed. Diluents were prepared by mixing the cryoprotectants at 10% concentration of the extenders (% v/v). Milt and diluents were mixed at a ratio of 1:9 for Alsevers solution and Kurokura-2; and 1:4 for urea egg-yolk, egg-yolk citrate, Ma and Mb. Egg-yolk citrate with 10% DMSO showed best performance producing 85 ± 1.6% post-thaw motility followed by 83±2% and 81±2.9% with Alsevers solution and urea egg-yolk respectively. Other extenders did not produce satisfactory results. Milt was diluted at six ratios (1:2, 1:4, 1:7, 1:9, 1:15 and 1:20) and 1:4 dilution showed the highest post-thaw motility for egg-yolk citrate and urea egg-yolk and 1:9 for Alsevers solution. Six cryoprotectant concentrations (5, 7, 10, 15, 20% and 30%) were investigated and 10% concentration produced the highest post-thaw motility. In breeding trials, sperm preserved with egg-yolk citrate plus DMSO as well as Alsevers solution plus DMSO fertilized eggs and produced hatchlings. The fertilization and hatching rates were 57 ± 7% and 46.5 ± 3.5% for egg-yolk citrate, and 33.5 ± 3.5% and 27±3% for Alsevers solution respectively. Fresh sperm yielded 76 ± 4% fertilization and 70.5 ± 5.5% hatching. The protocol developed through this study can be applied for long-term conservation of genetic materials of L. rohita and the cryopreserved sperm can be used in artificial breeding for generating new individuals.DOI: http://dx.doi.org/10.3329/pa.v22i1-2.16474 Progress. Agric. 22(1 & 2): 123-137, 2011
L'analyse et rien d'autre !
Authors: Baldacci Jean-Louis 
Published: September 2013

À partir du texte de C. Chabert « La belle freudienne », il s’agit d’interroger les incidences contre-transférentielles d’une indication d’analyse portée par une intime conviction. Le rapprochement entre conviction et potentialité mélancolique conduit, en référence à Freud et particulièrement au texte « Une névrose diabolique au xvii e  siècle », à la nécessité d’une « trahison » par la parole. La « trahison », en permettant une ouverture tiercéisante, ouvre un espace de rencontre entre parole associative et parole interprétative : elle remet en circulation une parole symbolisante.
Les fondements des concepts de base de la métapsychologie freudienne chez Winnicott ?
Authors: Girard Martine 
Published: September 2013

Dans un article récent, Fulgencio montre comment Winnicott a rejeté les concepts fondamentaux de la métapsychologie – pulsion, appareil psychique et libido – pour les remplacer par des concepts non spéculatifs en faveur d’une théorisation factuelle. Sont examinés ici quelques concepts winnicottiens suggérant qu’il n’a pas remplacé, mais fondé la métapsychologie sur des faits : la double dépendance de l’enfant par rapport aux soins. Freud n’a jamais méconnu la nécessité des soins maternels précoces, mais les considère comme allant de soi. En différenciant besoin du ça et besoin du moi, relation au ça et relation au moi, mère-objet et mère-environnement, Winnicott théorise ce que Freud tenait pour acquis : la fonction du holding comme cadre des expériences pulsionnelles et la fonction de présentation de l’objet comme condition de l’épreuve de réalité. De plus, avec le clivage des éléments féminins et masculins il est aussi capable d’une théorisation hautement spéculative visant à distinguer deux principes : l’être et le faire. Et s’il refuse effectivement l’instinct de mort, c’est dans la continuité de sa théorisation de la double dépendance. Ainsi l’auteur suggère que Winnicott n’a pas tant rejeté les concepts métapsychologiques que théorisé les conditions de leur utilisation et celles de la topique de l’intrapsychique.
Genotype-Dependent Effects of TGF-β1 on Mast Cell Function: Targeting the Stat5 Pathway
Authors: Fernando Josephine , Faber Travis W. , Pullen Nicholas A. , Falanga Yves T. , Kolawole Elizabeth Motunrayo , Oskeritzian Carole A. , Barnstein Brian O. , Bandara Geethani , Li Geqiang , Schwartz Lawrence B. , Spiegel Sarah , Straus David B. , Conrad Daniel H. , Bunting Kevin D. , Ryan John J. 
Published: September 2013

Abstract
We previously demonstrated that TGF-β1 suppresses IgE-mediated signaling in human and mouse mast cells in vitro, an effect that correlated with decreased expression of the high-affinity IgE receptor, FcεRI. The in vivo effects of TGF-β1 and the means by which it suppresses mast cells have been less clear. This study shows that TGF-β1 suppresses FcεRI and c-Kit expression in vivo. By examining changes in cytokine production concurrent with FcεRI expression, we found that TGF-β1 suppresses TNF production independent of FcεRI levels. Rather, IgE-mediated signaling was altered. TGF-β1 significantly reduced expression of Fyn and Stat5, proteins critical for cytokine induction. These changes may partly explain the effects of TGF-β1, because Stat5B overexpression blocked TGF-mediated suppression of IgE-induced cytokine production. We also found that Stat5B is required for mast cell migration toward stem cell factor, and that TGF-β1 reduced this migration. We found evidence that genetic background may alter TGF responses. TGF-β1 greatly reduced mast cell numbers in Th1-prone C57BL/6, but not Th2-prone 129/Sv mice. Furthermore, TGF-β1 did not suppress IgE-induced cytokine release and did increase c-Kit–mediated migration in 129/Sv mast cells. These data correlated with high basal Fyn and Stat5 expression in 129/Sv cells, which was not reduced by TGF-β1 treatment. Finally, primary human mast cell populations also showed variable sensitivity to TGF-β1–mediated changes in Stat5 and IgE-mediated IL-6 secretion. We propose that TGF-β1 regulates mast cell homeostasis, and that this feedback suppression may be dependent on genetic context, predisposing some individuals to atopic disease.
TIM-4 Has Dual Function in the Induction and Effector Phases of Murine Arthritis
Authors: Abe Yoshiyuki , Kamachi Fumitaka , Kawamoto Toshio , Makino Fumihiko , Ito Jun , Kojima Yuko , Moustapha Alaa El Din Hussein , Usui Yoshihiko , Yagita Hideo , Takasaki Yoshinari , Okumura Ko , Akiba Hisaya 
Published: September 2013

Abstract
T cell Ig and mucin domain (TIM)-4 is involved in immune regulation. However, the pathological function of TIM-4 has not been understood and remains to be clarified in various disease models. In this study, DBA/1 mice were treated with anti–TIM-4 mAb during the induction or effector phase of collagen-induced arthritis (CIA). Anti–TIM-4 treatment in the induction phase exacerbated the development of CIA. In vitro experiments suggest that CD4 T cells bind to TIM-4 on APCs, which induces inhibitory effect to CD4 T cells. In contrast, therapeutic treatment with anti–TIM-4 mAb just before or after the onset or even at later stage of CIA significantly suppressed the development and progression by reducing proinflammatory cytokines in the ankle joints without affecting T or B cell responses. Consistently, clinical arthritis scores of collagen Ab-induced arthritis, which is not mediated by T or B cells, were significantly reduced in anti–TIM-4–treated mice with a concomitant decrease of proinflammatory cytokines in the joints. In vitro, macrophages secreted proinflammatory cytokines in response to TIM-4-Ig protein and LPS, which were reduced by the anti–TIM-4 mAb. The anti–TIM-4 mAb also inhibited the differentiation and bone-resorbing activity of osteoclasts. These results indicate that TIM-4 has two distinct functions depending on the stage of arthritis. The therapeutic effect of anti–TIM-4 mAb on arthritis is mediated by the inhibition of proinflammatory cytokine production by inflammatory cells, osteoclast differentiation, and bone resorption, suggesting that TIM-4 might be an appropriate target for the therapeutic treatment of arthritis.
Human Memory Helios− FOXP3+ Regulatory T Cells (Tregs) Encompass Induced Tregs That Express Aiolos and Respond to IL-1β by Downregulating Their Suppressor Functions
Authors: Raffin Caroline , Pignon Pascale , Celse Clotilde , Debien Emilie , Valmori Danila , Ayyoub Maha 
Published: September 2013

Abstract
FOXP3+ regulatory T cells (Tregs) are critical regulators of self-tolerance and immune homeostasis. In mice and humans, two subsets of FOXP3+ Tregs have been defined based on their differential expression of Helios, a transcription factor of the Ikaros family. Whereas the origin, specificity, and differential function of the two subsets are as yet a source of controversy, their characterization thus far has been limited by the absence of surface markers to distinguish them. In this article, we show that human memory Helios+ and Helios− Tregs are phenotypically distinct and can be separated ex vivo based on their differential expression of IL-1RI, which is restricted to Helios− Tregs, in combination with CCR7. The two populations isolated using this strategy are distinct with respect to the expression of other Ikaros family members. Namely, whereas Eos, which has been reported to mediate FOXP3-dependent gene silencing, is expressed in Helios+ Tregs, Aiolos, which is involved in the differentiation of TH17 and induced Tregs, is instead expressed in Helios− Tregs. In addition, whereas both subsets are suppressive ex vivo, Helios− Tregs display increased suppressive capacity in comparison to Helios+ Tregs, but respond to IL-1β by downregulating their suppressive activity. Together, these data support the concept that human Helios− memory Tregs encompass induced Tregs that can readily respond to changes in the environment by modulating their suppressive capacity.
Ebf1 and c-Myb Repress Rag Transcription Downstream of Stat5 during Early B Cell Development
Authors: Timblin Greg A. , Schlissel Mark S. 
Published: September 2013

Abstract
The temporal control of RAG (Rag) expression in developing lymphocytes prevents DNA breaks during periods of proliferation that could threaten genomic integrity. In developing B cells, the IL-7R and precursor B cell Ag receptor (pre-BCR) synergize to induce proliferation and the repression of Rag at the protein and mRNA levels for a brief period following successful Ig H chain gene rearrangement. Whereas the mechanism of RAG2 protein downregulation is well defined, little is known about the pathways and transcription factors that mediate transcriptional repression of Rag. Using Abelson murine leukemia virus–transformed B cells to model this stage of development, we identified early B cell factor 1 (Ebf1) as a strong repressor of Rag transcription. Short hairpin RNA–mediated knockdown of either Ebf1 or its downstream target c-Myb was sufficient to induce Rag transcription in these highly proliferative cells. Ebf1 and c-Myb antagonize Rag transcription by negatively regulating the binding of Foxo1 to the Rag locus. Ebf1 accomplishes this through both direct negative regulation of Foxo1 expression and direct positive regulation of Gfi1b expression. Ebf1 expression is driven by the IL-7R downstream effector Stat5, providing a link between the negative regulation of Rag transcription by IL-7 and a novel repressive pathway involving Ebf1 and c-Myb.
Design and Management Features of Everyday Technology That Challenge Older Adults
Authors: Patomella Ann-Helen , Kottorp Anders , Nygård Louise 
Published: September 2013

Introduction: The management of everyday technologies could present too difficult a challenge for older adults with cognitive impairments, leading to their exclusion from participation. The present study aims to identify and describe features that make everyday technologies more or less difficult for older adults, whether with or without cognitive difficulties. Methods: A pre-existent, empirically generated hierarchy of more to less difficult everyday technologies was analysed. A mixed methods design was used to investigate predefined assumptions. Findings: Correlation analysis indicated that the more difficult everyday technologies required a higher frequency of skill actions; a positive correlation between the difficulty of an everyday technology and the need to perform actions in a logical sequence when using it was identified. The analysis indicated that feedback provided to users by less difficult everyday technologies consisted of different modes of stimuli (visual, auditory and tactile), while that from more difficult everyday technologies provided a high frequency of complex visual feedback. The less difficult everyday technologies were designed in a manner that was more intuitive; the design guided the user and fewer errors could be made. Conclusion: This study presents several features in the design of everyday technologies, and the skills required in using them, that could make their management more difficult for older adults.
A Survey Examining the Impact of Driving Cessation on People with Epilepsy in Ireland
Authors: Sullivan Clare , Buckley Sherrie 
Published: September 2013

Introduction: Driving is a job, a hobby and a form of transport that enables occupation. This research investigated the impact of the inability to drive due to epilepsy on community mobility and occupational participation, and compared both men and women's perceptions of the impact of driving cessation on romantic relationships and family involvement. Method: By means of an internet-based survey, data were collected concerning community mobility, personal impact of driving cessation, previous driving practices, epilepsy and demographics. Statistical analysis was performed on quantitative data and content analysis was used with responses to open questions. The survey received 95 eligible participants. Findings: Participants used public transport more than the general population; however, when unable to drive, being driving by friends or relatives was the main mode of transport. As a result, people experienced restricted access to the community, had to give up activities, had a reduced quality of life and a lowered self-esteem. Significantly more men than women felt that driving cessation had a negative impact on romantic relationships (p = 0.024) yet men and women did not differ significantly in their feelings about the impact of driving cessation on family involvement (p = 0.58). Conclusion: Independent access to the community is often dependent on the ability to drive.
The Effect of the Use of Discretion on Occupational Therapists' Professional Identity
Authors: Grant Aimee 
Published: September 2013

Introduction: In the United Kingdom, one strand of New Labour's welfare reform agenda was the introduction of the Condition Management Programme. In many areas of the United Kingdom, occupational therapists took a leading role in service delivery. This research article examines occupational therapists' use of discretion within the programme and its effect on their professional identity. Method: In-depth face-to-face interviews were undertaken with 13 staff members employed by the Condition Management Programme, including six occupational therapists. Interview transcripts were analysed thematically using ATLAS.ti 6 software as a data management tool. Findings: Within Condition Management Programmes, managers had a large amount of freedom in service design and created a service staffed in the majority by occupational therapists. While some decisions were made as part of a multidisciplinary team, occupational therapists were allowed considerable clinical autonomy in delivering the service. The impact of this autonomy on their professional identity is discussed. Conclusion: As a central part of the Condition Management Programme service, occupational therapists and other allied health professionals were allowed considerable autonomy. Concurrently, occupational therapists reported a strong professional identity. There is a need for further research within mainstream National Health Service departments to examine how discretion affects professional identity.
Systolic Anterior Motion: An Illustrative Case and Discussion of Management Strategy
Authors: Bardia Amit , Subramaniam Balachundhar 
Published: September 2013

ABSTRACT
Systolic anterior motion (SAM) of the mitral leaflets can lead to hemodynamic instability following mitral valve repair. Perioperative echocardiography plays a crucial role in the management of SAM. Perioperative echocardiography helps to (a) identify patients at high risk for SAM before repair, (b) assess SAM and diagnose severity and (c) follow-up management efficacy. An illustrative case is shown in this report to take the reader through the current management strategy of SAM following mitral valve repair.

How to cite this article
Bardia A, Subramaniam B. Systolic Anterior Motion: An Illustrative Case and Discussion of Management Strategy. J Perioper Echocardiogr 2013;1(2): 62-65.
COMPARATIVE INVESTIGATION OF THE DEVULCANIZATION PARAMETERS OF TIRE RUBBERS
Authors: Saiwari S. , Dierkes W. K. , Noordermeer J. W. M. 
Published: September 2013

ABSTRACT
The optimal process conditions for a high ratio of devulcanization to polymer degradation have been investigated for tire rubbers: styrene–butadiene rubber (SBR), butadiene rubber (BR), natural rubber (NR), and chloro-isobutylene–isoprene rubber (CIIR). These polymers all show their own particular breakdown characteristics. The temperature dependence of the breakdown mechanism was investigated by measuring sol fractions and cross-link densities. For SBR and BR, the highest reduction in cross-link density was found at a temperature of 220 °C, together with a moderate increase in sol content. According to the Horikx theory, which correlates sol fraction and a decrease in cross-link density, this is the result of a high degree of cross-link scission. Higher process temperatures result in a lower decrease in cross-link density due to recombination of active chain fragments. NR and CIIR show different behavior. Breakdown of NR in this temperature range results in an almost complete destruction of the polymer network; cross-link density is reduced to almost zero, and the sol fraction is close to 100%. The same result is found for CIIR at higher temperatures. Although different rubbers react via other devulcanization mechanisms, the best devulcanization conditions for whole passenger car tire material are optimized.
Determination of Methoxamine in Human Plasma and Urine by a Validated HPLC-Fluorescence Detection Method
Authors: Ulu Sevgi Tatar 
Published: September 2013

Abstract
A sensitive HPLC method for the determination of methoxamine in human plasma and urine is described. The method is based on derivatization of methoxamine with 4-chloro-7-nitrobenzofurazan in borate buffer of pH 9.5 to yield a yellow, fluorescent product. Isocratic HPLC separation was achieved on an Inertsil C18 column (250 × 4.6 mm id, 5 μm particle size) using the mobile phase methanol–water (60 + 40, v/v) at a flow rate of 1.2 mL/min. Fluorescence detection was used at excitation and emission wavelengths of 458 and 521 nm, respectively. The assay was linear over the concentration ranges of 10–250 and 20–300 ng/mL for plasma and urine, respectively. The LOD values were 3.3 and 6.8 ng/mL and the LOQ values were 10 and 20 ng/mL for plasma and urine, respectively. The extraction recoveries were more than 97.10%. After strict validation, the method indicated good performance in terms of linearity, sensitivity, precision, accuracy (recovery), robustness, and system suitability, and it was successfully applied to the determination of methoxamine in human plasma and urine.
Enantioselective Quantification of Atenolol in Mouse Plasma by High Performance Liquid Chromatography Using a Chiral Stationary Phase: Application to a Pharmacokinetic Study
Authors: Hefnawy Mohammed M , Al-Shehri Mona M , Abounassif Mohammed A , Mostafa Gamal A E 
Published: September 2013

Abstract
Enantiomeric resolution of atenolol was achieved on the HPLC vancomycin macrocyclic antibiotic chiral stationary phase Chirobiotic V. The polar ionic mobile phase consisted of methanol–glacial acetic acid–triethylamine (100 + 0.025 + 0.75, v/v/v) at a flow rate of 0.8 mL/min. Fluorescence detection at 275/305 nm for excitation and emission, respectively, was used. Plasma samples were purified using SPE on Oasis HLB cartridges. The calibration curves in plasma were linear over the range of 5–400 ng/mL (r = 0.999) for each enantiomer with an LOD of 1.0 ng/mL. The proposed method was validated in compliance with International Conference of Harmonization guidelines in terms of linearity, accuracy, precision, LOD, LOQ, and selectivity. The overall recoveries for S-(–)- and R-(+)-atenolol enantiomers from plasma were 95.0–99.5%; RSD ranged from 2.5 to 3.3%. The developed method was applied for the trace analysis of atenolol enantiomers in plasma and for the pharmacokinetic investigation of atenolol enantiomers in mouse plasma.
A Novel Validated Ultra-Performance Liquid Chromatography Method for Separation of Eszopiclone Impurities and its Degradants in Drug Products
Authors: Sharma Nitish , Rao Surendra Singh , Kumar Namala Durga Atchuta , Reddy Annarapu Malleswara 
Published: September 2013

Abstract
A selective, specific, and sensitive ultra-performance LC (UPLC) method was developed for determination of eszopiclone and its degradation products. The chromatographic separation was performed with a Waters ACQUITY UPLC system and BEH C18 column using gradient elution with mobile phases A and B. Mobile phase A was 0.01 M phosphate buffer with 0.2% (w/v) 1-octane sulfonic acid sodium salt as an ion pair reagent, adjusted pH 2.2 with orthophosphoric acid–acetonitrile (85 + 15, v/v). Mobile phase B was pH 2.2 buffer–acetonitrile (20 + 80, v/v). UV detection was performed at 303 nm. Eszopiclone and its impurities were chromatographed with a total run time of 13 min. A calibration study showed that the response for each of the impurities A, B, C, and D was linear between concentrations of 0.02 and 7.2 μg/mL (r2 ≥ 0.999). The method was validated over this range for precision, intermediate precision, accuracy, linearity, and specificity. For the precision study, RSD of each impurity was &lt;5% (n = 6). The method was found to be precise, accurate, linear, and specific. The proposed method was successfully used for determination of eszopiclone impurities in pharmaceutical preparations.