bluuebunny/crossref_metadata_2025_split huggingface dataset:
A semi-quantitative approach for modelling crop response to soil fertility: evaluation of the AquaCrop procedure
Authors: VAN GAELEN H. , TSEGAY A. , DELBECQUE N. , SHRESTHA N. , GARCIA M. , FAJARDO H. , MIRANDA R. , VANUYTRECHT E. , ABRHA B. , DIELS J. , RAES D. 
Published: October 2014

SUMMARYMost crop models make use of a nutrient-balance approach for modelling crop response to soil fertility. To counter the vast input data requirements that are typical of these models, the crop water productivity model AquaCrop adopts a semi-quantitative approach. Instead of providing nutrient levels, users of the model provide the soil fertility level as a model input. This level is expressed in terms of the expected impact on crop biomass production, which can be observed in the field or obtained from statistics of agricultural production. The present study is the first to describe extensively, and to calibrate and evaluate, the semi-quantitative approach of the AquaCrop model, which simulates the effect of soil fertility stress on crop production as a combination of slower canopy expansion, reduced maximum canopy cover, early decline in canopy cover and lower biomass water productivity. AquaCrop's fertility response algorithms are evaluated here against field experiments with tef (Eragrostis tef (Zucc.) Trotter) in Ethiopia, with maize (Zea mays L.) and wheat (Triticum aestivum L.) in Nepal, and with quinoa (Chenopodium quinoa Willd.) in Bolivia. It is demonstrated that AquaCrop is able to simulate the soil water content in the root zone, and the crop's canopy development, dry above-ground biomass development, final biomass and grain yield, under different soil fertility levels, for all four crops. Under combined soil water stress and soil fertility stress, the model predicts final grain yield with a relative root-mean-square error of only 11–13% for maize, wheat and quinoa, and 34% for tef. The present study shows that the semi-quantitative soil fertility approach of the AquaCrop model performs well and that the model can be applied, after case-specific calibration, to the simulation of crop production under different levels of soil fertility stress for various environmental conditions, without requiring detailed field observations on soil nutrient content.
Age, growth, longevity, mortality and reproductive biology ofDipturus oxyrinchus, (Chondrichthyes: Rajidae) off the Gulf of Gabès (Southern Tunisia, central Mediterranean)
Authors: Kadri Hasna , Marouani Sondes , Bradai Mahamed Nejmeddine , Bouaïn Abderrahmen , Morize Eric 
Published: October 2014

The age, growth, longevity, mortality and reproductive parameters were estimated forDipturus oxyrinchusfrom the Gulf of Gabès (Southern Tunisia, central Mediterranean Sea), collected monthly during 2007 from commercial fisheries. The present study provides the first data on age and growth of this species in Tunisian waters, as well as additional data on its reproduction. A total of 240 females (16.5–105 cm total length (TL)) and 280 males (15.5–95 cmTL) were examined to study the reproductive cycle. A subsample of 285 specimens (175 females and 110 males, ranging from 15.5 to 105 cmTL) were analysed to study the age and growth. The oldest female in this study was 25 yr and 105 cmTL, whereas the oldest male was 22 yr and 95 cm. The annual deposition of growth bands was verified by marginal increment and edge analysis. The von Bertalanffy growth parameters wereL∞ = 123.9 ± 2.56 cm,K = 0.08 ± 0.004 yr−1andt0 = −1.26 ± 0.04 10−1yr for females andL∞ = 102.1 ± 3.23 cm,K = 0.12 ± 0.007 10−3yr−1andt0 = −1.18 ± 0.03 10−1yr for males. Growth was not significantly different between sexes. The maturity size was 72.05 cm for males and 82.1 cm for females. The maturity age was estimated to be 11.95 and 13.96 years, respectively, for males and females. Size and age at maturity were not significantly different between sexes. The estimated longevity was 26.18 and 38.84 yr for males and females, respectively. Based on life history parameters, natural mortality was estimated at 0.53 yr−1for females and 0.41 yr−1for males.
THE AGORA OF GYTHEUM
Authors: Kanellopoulos Chrysanthos , Zavvou Eleni 
Published: October 2014

The Roman ‘Agora’ of Gytheum appears to be a large compound with an interior peristyle; its width is 52 m and its length at least 61 m. Excavations have revealed the remains of the north-east corner and a portion of the west wing. The extant architectural blocks allow the reconstruction of the colonnades. Column shafts were made of local red limestone and at least some of them were crowned with lotus-and-acanthus capitals. Three different interaxial column spacings are present. A single column must have stood on each corner of the peristyle. The style of the column capital suggests a date after the yearad100. The entablature is almost identical, in both style and dimensions, to the one found on the Captives' Facade at Corinth. The inscription on two epistyles reveals the private dedication of an exedra totheoi Sebastoiand the city. Most probably the exedra was behind the colonnaded space; the mention oftheoi Sebastoimight suggest imperial cult either in the exedra alone or both in the exedra and in the entire colonnaded compound. Quite possibly, and following A. Themos' suggestion, a large part of the colonnaded compound that is conventionally termed the ‘Agora’ can be identified with the Sebasteum/Caesareum of Gytheum; this latter structure is attested in other inscriptions from the city.
High-efficiency acceleration by the combination of laser and electrostatic field
Authors: Lin H. , Liu C.P. , Wang C. , Shen B.F. 
Published: October 2014

AbstractA new scheme of particle acceleration is verified by the investigation on single-body dynamics of charged particle in a compound field setup. This compound field setup contains a linear polarized laser field and a DC electric field which is along the direction of laser magnetic field. This setup can cause a charged particle to be of aperiodic motion and significantly high kinetic energy. Moreover, the contribution from the motion vertical to accelerating electric field is fully taken into account and is found to be essential to efficient acceleration. The efficiency of such a setup in acceleration is higher than that of a single laser.
Chemically induced enucleation of activated bovine oocytes: chromatin and microtubule organization and production of viable cytoplasts
Authors: Saraiva Naiara Zoccal , Oliveira Clara Slade , Leal Cláudia Lima Verde , de Lima Marina Ragagnin , Del Collado Maite , Vantini Roberta , Monteiro Fabio Morato , Niciura Simone Cristina Méo , Garcia Joaquim Mansano 
Published: October 2014

SummaryAs the standard enucleation method in mammalian nuclear transfer is invasive and damaging to cytoplast spatial organization, alternative procedures have been developed over recent years. Among these techniques, chemically induced enucleation (IE) is especially interesting because it does not employ ultraviolet light and reduces the amount of cytoplasm eliminated during the procedure. The objective of this study was to optimize the culture conditions with demecolcine of pre-activated bovine oocytes for chemically IE, and to evaluate nuclear and microtubule organization in cytoplasts obtained by this technique and their viability. In the first experiment, a negative effect on oocyte activation was verified when demecolcine was added at the beginning of the process, reducing activation rates by approximately 30%. This effect was not observed when demecolcine was added to the medium after 1.5 h of activation. In the second experiment, although a reduction in the number of microtubules was observed in most oocytes, these structures did not disappear completely during assessment. Approximately 50% of treated oocytes presented microtubule reduction at the end of the evaluation period, while 23% of oocytes were observed to exhibit the complete disappearance of these structures and 28% exhibited visible microtubules. These findings indicated the lack of immediate microtubule repolymerization after culture in demecolcine-free medium, a fact that may negatively influence embryonic development. However, cleavage rates of 63.6–70.0% and blastocyst yield of 15.5–24.2% were obtained in the final experiment, without significant differences between techniques, indicating that chemically induced enucleation produces normal embryos.
Sugar-sweetened beverages, vascular risk factors and events: a systematic literature review
Authors: Keller Amelie , Heitmann Berit L , Olsen Nanna 
Published: October 2014

AbstractObjectiveA high intake of sugar-sweetened beverages (SSB) has been linked to weight gain, obesity and type 2 diabetes; however, the influence on CVD risk remains unclear. Therefore, our objective was to summarize current evidence for an association between SSB consumption and cardiovascular risk factors and events.DesignThe article search was performed in August 2013. Two independent researchers performed the article search and selection, data extraction and quality assessment. Eligible studies reported the intake of SSB and one of the following outcomes: change in blood pressure, blood lipid or blood sugar, or CVD events such as stroke or myocardial infarction. Only intervention and longitudinal studies were included.SubjectsOnly studies in adults (aged 18+ years old) were considered.ResultsTwo of four prospective studies found clear direct associations between SSB consumption and CHD, while two of three studies, including both men and women, found direct associations between SSB consumption and stroke; however, the association was significant among women only. All included studies examining vascular risk factors found direct associations between SSB consumption and change in blood pressure, blood lipid or blood sugar.ConclusionsThe reviewed studies generally showed that SSB intake was related to vascular risk factors, whereas associations with vascular events were less consistent. Due to a limited number of published papers, especially regarding vascular events, the strength of the evidence is still limited and hence more studies are needed before firm conclusions can be made.
Direct optic nerve sheath (DONS) application of Schwann cells prolongs retinal ganglion cell survival in vivo
Authors: Guo L , Davis B , Nizari S , Normando E M , Shi H , Galvao J , Turner L , Shi J , Clements M , Parrinello S , Cordeiro M F 
Published: October 2014

AbstractCell-based therapies are increasingly recognized as a potential strategy to treat retinal neurodegenerative disease. Their administration, however, is normally indirect and complex, often with an inability to assess in real time their effects on cell death and their migration/integration into the host retina. In the present study, using a partial optic nerve transection (pONT) rat model, we describe a new method of Schwann cell (SC) delivery (direct application to injured optic nerve sheath, SC/DONS), which was compared with intravitreal SC delivery (SC/IVT). Both SC/DONS and SC/IVT were able to be assessedin vivousing imaging to visualize retinal ganglion cell (RGC) apoptosis and SC retinal integration. RGC death in the pONT model was best fitted to the one-phase exponential decay model. Although both SC/DONS and SC/IVT altered the temporal course of RGC degeneration in pONT, SC/DONS resulted in delayed but long-lasting effects on RGC protection, compared with SC/IVT treatment. In addition, their effects on primary and secondary degeneration, and axonal regeneration, were also investigated, by histology, whole retinal counting, and modelling of RGC loss. SC/DONS was found to significantly reduce RGC apoptosisin vivoand significantly increase RGC survival by targeting secondary rather than primary degeneration. Both SC/DONS and SC/IVT were found to promote RGC axonal regrowth after optic nerve injury, with evidence of GAP-43 expression in RGC somas and axons. SC/DONS may have the potential in the treatment of optic neuropathies, such as glaucoma. We show that SC transplantation can be monitored in real time and that the protective effects of SCs are associated with targeting secondary degeneration, with implications for translating cell-based therapies to the clinic.
Involvement of FoxO1 in the effects of follicle-stimulating hormone on inhibition of apoptosis in mouse granulosa cells
Authors: Shen M , Liu Z , Li B , Teng Y , Zhang J , Tang Y , Sun S-C , Liu H 
Published: October 2014

AbstractIn mammalian ovaries, follicular atresia occurs periodically and destroys almost all the follicles in the ovary. Follicle-stimulating hormone (FSH) acts as the primary survival factor during follicular atresia by preventing apoptosis in granulosa cells. FoxO1 is a critical factor in promoting follicular atresia and granulosa cell apoptosis. FSH inhibits the induction of FoxO1. In this report, we investigated the role of FSH-FoxO1 pathway in mouse follicular atresia. FSH dampened stress-induced apoptosis and the expression of FoxO1 and pro-apoptosis genes in mouse granulosa cells (MGCs). In contrast, overexpression of FoxO1 inhibited the viability of MGCs and induced the expression of endogenous FoxO1. The signaling cascades involved in regulating FoxO1 activity upon FSH treatment were identified using FSH signaling antagonists. Blocking protein kinase A (PKA), phosphatidylinositol-3 kinase (PI3K) or protein kinase B (AKT) restored the upregulation of FoxO1 and apoptotic signals, which was suppressed by FSH. Moreover, inhibition of PKA or PI3K impaired FSH-induced AKT activity, but inactivation of PI3K or AKT had little effect on PKA activity in the presence of FSH. Correspondingly, constitutive activation of FoxO1 (all three AKT sites were replaced by alanines) also promoted MGC apoptosis despite FSH administration. Furthermore, both luciferase reporter assays and chromatin immunoprecipitation assays showed that FoxO1 directly bound to a FoxO-recognized element site within the FoxO1 promoter and contributed to the regulation of FoxO1 expression in response to FSH. Taken together, we propose a novel model in which FSH downregulates FoxO1-dependent apoptosis in MGCs by coordinating the PKA–PI3K–AKT–FoxO1 axis and FoxO1–FoxO1 positive feedback.
Inhibition of human positive cofactor 4 radiosensitizes human esophageal squmaous cell carcinoma cells by suppressing XLF-mediated nonhomologous end joining
Authors: Qian D , Zhang B , Zeng X-L , Le Blanc J M , Guo Y-H , Xue C , Jiang C , Wang H-H , Zhao T-S , Meng M-B , Zhao L-J , Hao J-H , Wang P , Xie D , Lu B , Yuan Z-Y 
Published: October 2014

AbstractRadiotherapy has the widest application to esophageal squamous cell carcinoma (ESCC) patients. Factors associated with DNA damage repair have been shown to function in cell radiosensitivity. Human positive cofactor 4 (PC4) has a role in nonhomologous end joining (NHEJ) and is involved in DNA damage repair. However, the clinical significance and biological role of PC4 in cancer progression and cancer cellular responses to chemoradiotherapy (CRT) remain largely unknown. The aim of the present study was to investigate the potential roles of PC4 in the radiosensitivity of ESCC. In this study, we showed that knockdown of PC4 substantially increased ESCC cell sensitivity to ionizing radiation (IR) both in vitro and in vivo and enhanced radiation-induced apoptosis and mitotic catastrophe (MC). Importantly, we demonstrated that silencing of PC4 suppressed NHEJ by downregulating the expression of XLF in ESCC cells, whereas reconstituting the expression of XLF protein in the PC4-knockdown ESCC cells restored NHEJ activity and radioresistance. Moreover, high expression of PC4 positively correlated with ESCC resistance to CRT and was an independent predictor for short disease-specific survival of ESCC patients in both of our cohorts. These findings suggest that PC4 protects ESCC cells from IR-induced death by enhancing the NHEJ-promoting activity of XLF and could be used as a novel radiosensitivity predictor and a promising therapeutic target for ESCCs.
HDAC8 and STAT3 repress BMF gene activity in colon cancer cells
Authors: Kang Y , Nian H , Rajendran P , Kim E , Dashwood W M , Pinto J T , Boardman L A , Thibodeau S N , Limburg P J , Löhr C V , Bisson W H , Williams D E , Ho E , Dashwood R H 
Published: October 2014

AbstractHistone deacetylase (HDAC) inhibitors are undergoing clinical trials as anticancer agents, but some exhibit resistance mechanisms linked to anti-apoptotic Bcl-2 functions, such as BH3-only protein silencing. HDAC inhibitors that reactivate BH3-only family members might offer an improved therapeutic approach. We show here that a novel seleno-α-keto acid triggers global histone acetylation in human colon cancer cells and activates apoptosis in a p21-independent manner. Profiling of multiple survival factors identified a critical role for the BH3-only member Bcl-2-modifying factor (Bmf). On the corresponding BMF gene promoter, loss of HDAC8 was associated with signal transducer and activator of transcription 3 (STAT3)/specificity protein 3 (Sp3) transcription factor exchange and recruitment of p300. Treatment with a p300 inhibitor or transient overexpression of exogenous HDAC8 interfered with BMF induction, whereas RNAi-mediated silencing of STAT3 activated the target gene. This is the first report to identify a direct target gene of HDAC8 repression, namely, BMF. Interestingly, the repressive role of HDAC8 could be uncoupled from HDAC1 to trigger Bmf-mediated apoptosis. These findings have implications for the development of HDAC8-selective inhibitors as therapeutic agents, beyond the reported involvement of HDAC8 in childhood malignancy.
Artesunate induces necrotic cell death in schwannoma cells
Authors: Button R W , Lin F , Ercolano E , Vincent J H , Hu B , Hanemann C O , Luo S 
Published: October 2014

AbstractEstablished as a potent anti-malaria medicine, artemisinin-based drugs have been suggested to have anti-tumour activity in some cancers. Although the mechanism is poorly understood, it has been suggested that artemisinin induces apoptotic cell death. Here, we show that the artemisinin analogue artesunate (ART) effectively induces cell death in RT4 schwannoma cells and human primary schwannoma cells. Interestingly, our data indicate for first time that the cell death induced by ART is largely dependent on necroptosis. ART appears to inhibit autophagy, which may also contribute to the cell death. Our data in human schwannoma cells show that ART can be combined with the autophagy inhibitor chloroquine (CQ) to potentiate the cell death. Thus, this study suggests that artemisinin-based drugs may be used in certain tumours where cells are necroptosis competent, and the drugs may act in synergy with apoptosis inducers or autophagy inhibitors to enhance their anti-tumour activity.
Enrichment of c-Met+ tumorigenic stromal cells of giant cell tumor of bone and targeting by cabozantinib
Authors: Liu L , Aleksandrowicz E , Fan P , Schönsiegel F , Zhang Y , Sähr H , Gladkich J , Mattern J , Depeweg D , Lehner B , Fellenberg J , Herr I 
Published: October 2014

AbstractGiant cell tumor of bone (GCTB) is a very rare tumor entity, which is little examined owing to the lack of established cell lines and mouse models and the restriction of available primary cell lines. The stromal cells of GCTB have been made responsible for the aggressive growth and metastasis, emphasizing the presence of a cancer stem cell population. To identify and target such tumor-initiating cells, stromal cells were isolated from eight freshly resected GCTB tissues. Tumorigenic properties were examined by colony and spheroid formation, differentiation, migration, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, immunohistochemistry, antibody protein array, Alu in situ hybridization, FACS analysis and xenotransplantation into fertilized chicken eggs and mice. A sub-population of the neoplastic stromal cells formed spheroids and colonies, differentiated to osteoblasts, migrated to wounded regions and expressed the metastasis marker CXC-chemokine receptor type 4, indicating self-renewal, invasion and differentiation potential. Compared with adherent-growing cells, markers for pluripotency, stemness and cancer progression, including the CSC surface marker c-Met, were enhanced in spheroidal cells. This c-Met-enriched sub-population formed xenograft tumors in fertilized chicken eggs and mice. Cabozantinib, an inhibitor of c-Met in phase II trials, eliminated CSC features with a higher therapeutic effect than standard chemotherapy. This study identifies a c-Met+ tumorigenic sub-population within stromal GCTB cells and suggests the c-Met inhibitor cabozantinib as a new therapeutic option for targeted elimination of unresectable or recurrent GCTB.
Deficient Wnt signalling triggers striatal synaptic degeneration and impaired motor behaviour in adult mice
Authors: Galli Soledad , Lopes Douglas M. , Ammari Rachida , Kopra Jaakko , Millar Sarah E. , Gibb Alasdair , Salinas Patricia C. 
Published: October 2014

AbstractSynapse degeneration is an early and invariant feature of neurodegenerative diseases. Indeed, synapse loss occurs prior to neuronal degeneration and correlates with the symptom severity of these diseases. However, the molecular mechanisms that trigger synaptic loss remain poorly understood. Here we demonstrate that deficient Wnt signalling elicits synaptic degeneration in the adult striatum. Inducible expression of the secreted Wnt antagonist Dickkopf1 (Dkk1) in adult mice (iDkk1) decreases the number of cortico-striatal glutamatergic synapses and of D1 and D2 dopamine receptor clusters. Synapse loss occurs in the absence of axon retraction or cell death. The remaining excitatory terminals contain fewer synaptic vesicles and have a reduced probability of evoked transmitter release. IDkk1 mice show impaired motor coordination and are irresponsive to amphetamine. These studies identify Wnts as key endogenous regulators of synaptic maintenance and suggest that dysfunction in Wnt signalling contributes to synaptic degeneration at early stages in neurodegenerative diseases.
Probing excitonic states in suspended two-dimensional semiconductors by photocurrent spectroscopy
Authors: Klots A. R. , Newaz A. K. M. , Wang Bin , Prasai D. , Krzyzanowska H. , Lin Junhao , Caudel D. , Ghimire N. J. , Yan J. , Ivanov B. L. , Velizhanin K. A. , Burger A. , Mandrus D. G. , Tolk N. H. , Pantelides S. T. , Bolotin K. I. 
Published: October 2014

AbstractThe optical response of semiconducting monolayer transition-metal dichalcogenides (TMDCs) is dominated by strongly bound excitons that are stable even at room temperature. However, substrate-related effects such as screening and disorder in currently available specimens mask many anticipated physical phenomena and limit device applications of TMDCs. Here, we demonstrate that that these undesirable effects are strongly suppressed in suspended devices. Extremely robust (photogain > 1,000) and fast (response time < 1 ms) photoresponse allow us to study, for the first time, the formation, binding energies and dissociation mechanisms of excitons in TMDCs through photocurrent spectroscopy. By analyzing the spectral positions of peaks in the photocurrent and by comparing them with first-principles calculations, we obtain binding energies, band gaps and spin-orbit splitting in monolayer TMDCs. For monolayer MoS2, in particular, we obtain an extremely large binding energy for band-edge excitons, Ebind ≥ 570 meV. Along with band-edge excitons, we observe excitons associated with a van Hove singularity of rather unique nature. The analysis of the source-drain voltage dependence of photocurrent spectra reveals exciton dissociation and photoconversion mechanisms in TMDCs.