bluuebunny/crossref_metadata_2025_split huggingface dataset:
Les territoires ruraux au Québec : vers un modèle de développement territorial durable
Authors: Jean Bruno 
Published: December 2012

La Québec étant une « société neuve » donne à voir différents modèles d’occupation de ses différents territoires. Sa structuration spatiale présente une région centrale, des régions intermédiaires et des régions périphériques et on observe une déconnexion entre celles-ci et notre métropole. Trois modèles de développement rural ont caractérisé l’histoire québécoise : le développement territorial par la petite propriété, par la grande entreprise et par l’État. Ils ont imprégnés la conscience collective et influencent les représentations différenciées du développement territorial. À la suite du succès limité des politiques descendantes de développement régional, une nouvelle approche basée sur la mobilisation des acteurs locaux a émergé. Dans ce renversement de l’action publique, la ruralité de refaire surface et de devenir un objet de la politique publique. Nous montrerons alors que la Politique nationale de la ruralité du Québec est une politique innovatrice qui propose un véritable modèle de développement territorial durable.
Le développement local au Québec : trois décennies de contributions
Authors: Joyal André 
Published: December 2012

Au Québec, l’expression « développement local » (DL) a remplacé l’expression « développement économique communautaire » qui durant les années 70 se rapportait à des initiatives à la fois dotées d’une finalité sociale et économique, mises de l’avant par des acteurs victimes de la conjoncture économique et désireuses d’assumer une plus grande responsabilité de leur avenir immédiat. Nous visons ici à montrer, à travers trois décennies de contributions, qu’activistes et universitaires québécois, tout en ayant des affinités et des objectifs rapprochés, se partagent en deux catégories : ceux que l’on pourrait désigner comme étant adeptes du DL communautaire dit de type « progressif » avec les secteurs coopératif et associatif comme pierre d’assise, et ceux qui misent sur l’entrepreneuriat local de type privé associé à un DL dit de type « libéral ». Ceci, en s’interrogeant où en est-on au plan des efforts de théorisation ou de modélisation ?
Canada Basin Revealed
Authors: Mosher D.C. , Shimeld J. , Hutchinson D. , Chian D. , Lebedova-Ivanova N. , Jackson R. 
Published: December 2012

Abstract
More than 15,000 line-km of new regional seismic reflection and refractiondata in the western Arctic Ocean provide insights into the tectonic andsedimentologic history of Canada Basin, permitting development of new geologicunderstanding in one of Earth's last frontiers. These new data support arotational opening model for southern Canada Basin. There is a central basementridge possibly representing an extinct spreading center with oceanic crustalvelocities and blocky basement morphology characteristic of spreading centrecrust surrounding this ridge. Basement elevation is lower in the south, mostlydue to sediment loading subsidence. The sedimentary succession is thickest inthe southern Beaufort Sea region, reaching more than 15 km, and generally thinsto the north and west. In the north, grabens and half-grabens are indicative ofextension. Alpha-Mendeleev Ridge is a large igneous province in northernAmerasia Basin, presumably emplaced synchronously with basin formation. Itoverprints most of northern Canada Basin structure. The seafloor andsedimentary succession of Canada Basin is remarkably flat-lying in its centralregion, with little bathymetric change over most of its extent. Reflectionsthat correlate over 100s of kms comprise most of the succession and on-lapbathymetric and basement highs. They are interpreted as representing depositsfrom unconfined turbidity current flows. Sediment distribution patterns reflectchanging source directions during the basin's history. Initially, probably lateCretaceous to Paleocene synrift sediments sourced from the Alaska andMackenzie-Beaufort margins. This unit shows a progressive series of onlapunconformities with a younging trend towards Alpha and Northwind ridges, likelya response to contemporaneous subsidence. Sediment source direction appeared toshift to the Canadian Arctic Archipelago margin for the Eocene and Oligocene, likely due to uplift of Arctic islands during the Eurekan Orogeny. The finalstage of sedimentation appears to be from the Mackenzie-Beaufort region for theMiocene and Pliocene when drainage patterns shifted in the Yukon and Alaska tothe Mackenzie valley. Upturned reflections at onlap positions may indicatesyn-depositional subsidence. There is little evidence, at least at a regionalseismic data scale, of contemporaneous or post-depositional sediment reworking, suggesting little large-scale geostrophic or thermohaline-driven bottom currentactivity.
Novel Cellular Targets of AhR Underlie Alterations in Neutrophilic Inflammation and Inducible Nitric Oxide Synthase Expression during Influenza Virus Infection
Authors: Wheeler Jennifer L. Head , Martin Kyle C. , Lawrence B. Paige 
Published: December 2012

Abstract
The underlying reasons for variable clinical outcomes from respiratory viral infections remain uncertain. Several studies suggest that environmental factors contribute to this variation, but limited knowledge of cellular and molecular targets of these agents hampers our ability to quantify or modify their contribution to disease and improve public health. The aryl hydrocarbon receptor (AhR) is an environment-sensing transcription factor that binds many anthropogenic and natural chemicals. The immunomodulatory properties of AhR ligands are best characterized with extensive studies of changes in CD4+ T cell responses. Yet, AhR modulates other aspects of immune function. We previously showed that during influenza virus infection, AhR activation modulates neutrophil accumulation in the lung, and this contributes to increased mortality in mice. Enhanced levels of inducible NO synthase (iNOS) in infected lungs are observed during the same time frame as AhR-mediated increased pulmonary neutrophilia. In this study, we evaluated whether these two consequences of AhR activation are causally linked. Reciprocal inhibition of AhR-mediated elevations in iNOS and pulmonary neutrophilia reveal that although they are contemporaneous, they are not causally related. We show using Cre/loxP technology that elevated iNOS levels and neutrophil number in the infected lung result from separate, AhR-dependent signaling in endothelial and respiratory epithelial cells, respectively. Studies using mutant mice further reveal that AhR-mediated alterations in these innate responses to infection require a functional nuclear localization signal and DNA binding domain. Thus, gene targets of AhR in non-hematopoietic cells are important new considerations for understanding AhR-mediated changes in innate anti-viral immunity.
Antibody Binding to Cryptococcus neoformans Impairs Budding by Altering Capsular Mechanical Properties
Authors: Cordero Radames J. B. , Pontes Bruno , Frases Susana , Nakouzi Antonio S. , Nimrichter Leonardo , Rodrigues Marcio L. , Viana Nathan B. , Casadevall Arturo 
Published: December 2012

Abstract
Abs to microbial capsules are critical for host defense against encapsulated pathogens, but very little is known about the effects of Ab binding on the capsule, apart from producing qualitative capsular reactions (“quellung” effects). A problem in studying Ab–capsule interactions is the lack of experimental methodology, given that capsules are fragile, highly hydrated structures. In this study, we pioneered the use of optical tweezers microscopy to study Ab–capsule interactions. Binding of protective mAbs to the capsule of the fungal pathogen Cryptococcus neoformans impaired yeast budding by trapping newly emerging buds inside the parental capsule. This effect is due to profound mAb-mediated changes in capsular mechanical properties, demonstrated by a concentration-dependent increase in capsule stiffness. This increase involved mAb-mediated cross-linking of capsular polysaccharide molecules. These results provide new insights into Ab-mediated immunity, while suggesting a new nonclassical mechanism of Ab function, which may apply to other encapsulated pathogens. Our findings add to the growing body of evidence that Abs have direct antimicrobial functions independent of other components of the immune system.
MicroRNA-31 Is Overexpressed in Psoriasis and Modulates Inflammatory Cytokine and Chemokine Production in Keratinocytes via Targeting Serine/Threonine Kinase 40
Authors: Xu Ning , Meisgen Florian , Butler Lynn M. , Han Gangwen , Wang Xiao-Jing , Söderberg-Nauclér Cecilia , Ståhle Mona , Pivarcsi Andor , Sonkoly Enikö 
Published: December 2012

Abstract
Psoriasis is characterized by a specific microRNA expression profile, distinct from that of healthy skin. MiR-31 is one of the most highly overexpressed microRNAs in psoriasis skin; however, its biological role in the disease has not been studied. In this study, we show that miR-31 is markedly overexpressed in psoriasis keratinocytes. Specific inhibition of miR-31 suppressed NF-κB–driven promoter luciferase activity and the basal and TNF-α–induced production of IL-1β, CXCL1/growth-related oncogene-α, CXCL5/epithelial-derived neutrophil-activating peptide 78, and CXCL8/IL-8 in human primary keratinocytes. Moreover, interference with endogenous miR-31 decreased the ability of keratinocytes to activate endothelial cells and attract leukocytes. By microarray expression profiling, we identified genes regulated by miR-31 in keratinocytes. Among these genes, we identified serine/threonine kinase 40 (STK40), a negative regulator of NF-κB signaling, as a direct target for miR-31. Silencing of STK40 rescued the suppressive effect of miR-31 inhibition on cytokine/chemokine expression, indicating that miR-31 regulates cytokine/chemokine expression via targeting STK40 in keratinocytes. Finally, we demonstrated that TGF-β1, a cytokine highly expressed in psoriasis epidermis, upregulated miR-31 expression in keratinocytes in vitro and in vivo. Collectively, our findings suggest that overexpression of miR-31 contributes to skin inflammation in psoriasis lesions by regulating the production of inflammatory mediators and leukocyte chemotaxis to the skin. Our data indicate that inhibition of miR-31 may be a potential therapeutic option in psoriasis.
Rapid Determination of Free Fatty Acid Content in Waste Deodorizer Distillates Using Single Bounce-Attenuated Total Reflectance-FTIR Spectroscopy
Authors: Naz Saba , Sherazi Sayed Tufail Hussain , Talpur Farah N , Mahesar Sarfaraz A , Kara Huseyin 
Published: December 2012

Abstract
A simple, rapid, economical, and environmentally friendly analytical method was developed for the quantitative assessment of free fatty acids (FFAs) present in deodorizer distillates and crude oils by single bounce-attenuated total reflectance-FTIR spectroscopy. Partial least squares was applied for the calibration model based on the peak region of the carbonyl group (C=O) from 1726 to 1664 cm–1 associated with the FFAs. The proposed method totally avoided the use of organic solvents or costly standards and could be applied easily in the oil processing industry. The accuracy of the method was checked by comparison to a conventional standard American Oil Chemists' Society (AOCS) titrimetric procedure, which provided good correlation (R = 0.99980), with an SD of ±0.05%. Therefore, the proposed method could be used as an alternate to the AOCS titrimetric method for the quantitative determination of FFAs especially in deodorizer distillates.
Comparison of Activated Carbon and Oxidized Multiwalled Carbon Nanotubes Modified with Bis(3-Nitrobenzylidene)-1,2-Ethanediamine for Enrichment of Trace Amounts of Some Metal Ions
Authors: Ghaedi Mehrorang , Montazerozohori Mortaza , Tabatabie Maryann , Noormohamadi Hamid , Haghighi Alireza Borhan 
Published: December 2012

Abstract
The efficiency of modified activated carbon (AC) and multiwalled carbon nanotubes (MWCNTs) for the separation/preconcentration and determination of Co, Cd, Pb, Zn, and Cu following their complexation by bis(3-nitrobenzylidene)-1,2-ethanediamine has been described and compared. A one-at-a-time optimization method investigated the influence of various parameters that significantly influence the recoveries of the studied metal ions. At the optimum values of all variables, the response was linear over the range of 0.01–0.3 μg/mL, and detection limit (3 SDb/m, n = 10) was between 1.41–2.05 ng/mL for both sorbents while the preconcentration factor was 100 for AC and 500 for MWCNTs. The method was successfully applied for preconcentration and determination of trace amount of the aforementioned ions in various real samples such as orange, lettuce, bread, and pear.
Thin-Layer Chromatography-Densitometry Analysis of Dimethoxyphenylbutadiene Content in Zingiber cassumunar Rhizomes
Authors: Tangyuenyongwatana Prasan , Keeratinijakal Vichien , Gritsanapan Wandee 
Published: December 2012

Abstract
Zingiber cassumunar (Zingiberaceae) has been widely used as a traditional medicine in southeast Asia, especially Thailand, for treatment of asthma, inflammation, and joint pain. (E)-4-(3,4-dimethoxyphenyl)butadiene, or DMPBD, a major component in the rhizome, has been reported to be an active anti-inflammatory agent. This research developed a TLC-densitometry method for the simultaneous quantification of DMPBD in the rhizome extracts of four varieties of Z. cassumunar. DMPBD was found in the range of 1.80 ± 0.01 to 3.23 ± 0.10% (w/w). The method was validated for linearity, precision, accuracy, robustness, LOD, and LOQ. The linearity was indicated by a good regression coefficient 0.9940 and covered the range of 130 to 703 ng/band. Intraday and interday precision (RSD) were less than 2%. Accuracy of the method was evaluated by a recovery study conducted at three different levels; the average recovery was 103.1%. The LOD and LOQ were 10 and 40 ng, respectively. The proposed TLC-densitometry method was simple, precise, specific, sensitive, and inexpensive. This method can be used for routine QC of raw materials of Z. cassumunar rhizomes, extracts, and its products, especially Prasaplai, a Thai traditional medicine for relieving dysmenorrhea.
Evaluation of Cyanocobalamin in Multivitamin Tablets and Their Standard Reference Material 3280 by HPLC with Visible Detection
Authors: Matsumoto Teruki , Takebayashi J Un , Ishimi Yoshiko , Ozawa Chihiro , Sano Akira , Hirota T Akashi , Endoh Kaori 
Published: December 2012

Abstract
As a part of the development of an alternative to microbiological assay for vitamin B12, we performed a quantitative analysis of cyanocobalamin (CN-cbl) in a National Institute of Standards and Technology Standard Reference Material (SRM 3280) by HPLC. Using this method, the observed value (4.64 μg/g) of CN-cbl in SRM 3280 was found to be in good agreement with the certified value (4.80 μg/g). The accuracy was over 95%, with a corresponding measurement precision value of 5%. To evaluate the applicability of the method on commercial multivitamin tablets, the method was applied to a variety of these samples. The present method has a good accuracy and precision to evaluate CN-cbl with respect to all of the examined tablets.
Validation of HPTLC and HPLC Methods for the Quantitative Determination of Allyl Disulfide in Some Polyherbal Oils
Authors: Dubey Nitin , Dubey Nidhi , Mehta Rajendra 
Published: December 2012

Abstract
Allium sativum L (garlic) is an essential component of many polyherbal oils used in traditional systems of medicine. Allyl disulfide has been a major component found in vegetable oil macerate of garlic, and can be used as reliable marker for determination of garlic in oil macerates of garlic. The HPLC separation of allyl disulfide was achieved on a Phenomenex Luna C18 (25 cm × 4.6 mm id × 5 μm particle size) column using acetonitrile–water–tetrahydrofuran (70 + 27 + 3, v/v/v) mobile phase at a flow rate of 1.0 mL/min. Quantitation was achieved with UV detection at 298 nm over the concentration range 8–48 μg/mL. HPTLC separation of allyl disulfide was achieved on an aluminum-backed layer of silica gel 60 F254 using n-hexane mobile phase. Quantitation was achieved by densitometric analysis at 298 nm over the 200–1200 ng/band concentration range. The methods were validated according to International Conference on Harmonization guidelines.
Quantitative Determination of Cucurbitane-Type Triterpenes and Triterpene Glycosides in Dietary Supplements Containing Bitter Melon (Momordica charantia) by HPLC-MS/MS
Authors: Ma Jun , Krynitsky Alexander J , Grundel Erich , Rader Jeanne I 
Published: December 2012

Abstract
Momordica charantia L. (Cucurbitaceae), commonly known as bitter melon, is widely cultivated in many tropical and subtropical areas of the world. It is a common food staple; its fruits, leaves, seeds, stems, and roots also have a long history of use in traditional medicine. In the United States, dietary supplements labeled as containing bitter melon can be purchased over-the-counter and from Internet suppliers. Currently, no quantitative analytical method is available for monitoring the content of cucurbitane-type triterpenes and triterpene glycosides, the major constituents of bitter melon, in such supplements. We investigated the use of HPLC-electrospray ionization (ESI)-MS/MS for the quantitative determination of such compounds in dietary supplements containing bitter melon. Values for each compound obtained from external calibration were compared with those obtained from the method of standard additions to address matrix effects associated with ESI. In addition, the cucurbitane-type triterpene and triterpene glycoside contents of two dietary supplements determined by the HPLC-ESI-MS/MS method with standard additions were compared with those measured by an HPLC method with evaporative light scattering detection, which was recently developed for quantification of such compounds in dried fruits of M. charantia. The contents of five cucurbitane-type triterpenes and triterpene glycosides in 10 dietary supplements were measured using the HPLC-ESI-MS/MS method with standard additions. The total contents of the five compounds ranged from 17 to 3464 μg/serving.
Method Development and Validation for Ultra-High-Pressure LC/MS/MS Determination of Hop Prenylflavonoids in Human Serum
Authors: Yuan Yang , Qiu Xi , Nikolic Dejan , Dahl Jeffrey H , van Breemen Richard B 
Published: December 2012

Abstract
Hops (Humulus lupulus L.) are used in the brewing of beer, and hop extracts containing prenylated compounds, such as xanthohumol (XN) and 8-prenylnaringenin (8-PN), are under investigation as dietary supplements for cancer chemoprevention and the management of hot flashes in menopausal women. To facilitate clinical studies of hop safety and efficacy, a selective, sensitive, and fast ultra-high-pressure LC (UHPLC) tandem MS method was developed and validated for the simultaneous determination of the hop prenylflavonoids XN, isoxanthohumol (IX), 6-prenylnaringenin (6-PN), and 8-PN in human serum. The analytical method requires 300 μL of human serum, which is processed using liquid–liquid extraction. UHPLC separation was carried out in 2.5 min with gradient elution using an RP C18 column containing 1.6 μm particle size packing material. Prenylflavonoids were measured using negative ion electrospray MS with collision-induced dissociation and selected reaction monitoring. The method was validated and showed good accuracy and precision with an LOQ of 0.50 ng/mL for XN (1.4 nM) and 1.0 ng/mL for 6-PN, 8-PN (2.94 nM), and IX (2.82 nM) in serum.
Changes of the Wax Contents in Mixtures of Olive Oils as Determined by Gas Chromatography with a Flame Ionization Detector
Authors: Hodaifa Gassan , Nieto Leopoldo Martínez , Lozano Juan L , Sánchez Sebastián 
Published: December 2012

Abstract
Mixing of refined olive-pomace oil with virgin olive oil is a fraud that has been tried often. Normally, the tests that detected the fraud were determinations of wax esters, erythrodiol+uvaol, and stigmastadienes contents. The most common is the determination of wax esters content (extra virgin olive oil is very poor in wax esters, usually less than 100 mg/kg). In this work, the variations of individual wax esters (C40, C42, C44, and C46), with different degrees of unsaturation content, and total wax esters were studied when extra virgin olive oil and refined pomace-olive oil were mixed. The following mixtures were prepared: extra virgin olive oil plus 1, 2, 4, 6, 8, 10, 12, 15, 18, 25, 35, 45, 50, and 80% of refined olive-pomace oil. In all cases, individual and total wax ester content variation was linear with increasing percentage of refined olive-pomace oil in the mixture. The variation of the total wax esters content can be adjusted according to the equation: Total wax esters, mg/kg = 14.3 × (% refined olive-pomace oil) + 83.9.
Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation: First Action 2011.06
Authors: Szpylka John , De Vries Jon , Cheney Andrea , House Steve 
Published: December 2012

Abstract
The method for “Determination of Total Folates in Infant Formula and Adult Nutritionals by Trienzyme Extraction and UPLC-MS/MS Quantitation” was submitted to the Folate Working Group for consideration for adoption as Official First Action by AOAC INTERNATIONAL. This method uses ultra performance liquid chromatography/tandem mass spectrometry (UPLC-MS/MS) to determine the total folates in infant formulas and adult nutritionals after trienzyme digestion. Deconjugation of the various folate polyglutamates to folate monoglutamates is achieved by using rat plasma conjugase after the sample digestion with protease and α-amylase during the trienzyme digestion process. This method shows linearity of folate concentrations in the range of 10–19 100 μg/100 g. Extension of the range to cover folate concentrations of 5–2 000 000 μg/100 g can be achieved with appropriate adjustment of the sample weight and SPE cleanup loading volume. The recoveries ranged from 94.10 to 101.34%.
Management and Support of Shared Integrated Library Systems
Authors: Vaughan Jason , Costello Kristen 
Published: December 2012

The University of Nevada, Las Vegas (UNLV) University Libraries has hosted and managed a shared integrated library system (ILS) since 1989. The system and the number of partner libraries sharing the system has grown significantly over the past two decades. Spurred by the level of involvement and support contributed by the host institution, the authors administered a comprehensive survey to current Innovative Interfaces libraries. Research findings are combined with a description of UNLV’s local practices to provide substantial insights into shared funding, support, and management activities associated with shared systems.
Building an Open Source Institutional Repository at a Small Law School Library: Is it Realistic or Unattainable?
Authors: Wang Fang 
Published: December 2012

Digital preservation activities among law libraries have largely been limited by a lack of funding, staffing and expertise. Most law school libraries that have already implemented an Institutional Repository (IR) chose proprietary platforms because they are easy to set up, customize, and maintain with the technical and development support they provide. The Texas Tech University School of Law Digital Repository is one of the few law school repositories in the nation that is built on the DSpace open source platform.1 The repository is the law school’s first institutional repository in history. It was designed to collect, preserve, share and promote the law school’s digital materials, including research and scholarship of the law faculty and students, institutional history, and law-related resources. In addition, the repository also serves as a dark archive to house internal records.