bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leakage of Phosphatases and Fertility of Buck Semen Cryopreserved under Different Freezing Modes
Authors: Sharma Sunanda , Sharma Narendra Kumar , Kataria Nalini , Sinha N K 
Published: December 2013

Present study was conducted on 160 ejaculates collected at weekly interval by artificial vagina method from 13 adult Sirohi bucks. Pooled ejaculates were diluted with Tris-egg yolk-citric acid-fructose-glycerol extender (1:4), filled and sealed in straws. Few straws of diluted semen were thawed (40° C/15 seconds) and assessed for acid and alkaline phosphatases (ACP and AKP) in seminal plasma of diluted semen (Control Group). Remaining semen straws were randomly grouped to constitute freezing mode groups (M1, M2, M2, and M4) and processed further for cryo-preservation of semen. Accordingly diluted semen straws were cooled @-4° C/minute from 25° C up to 5° C thereafter equilibrated for 2 hours and frozen up to -160° C @ 15, 20, 25 and 300 C/minute for M1, M2, M3 and M4 groups respectively. These frozen straws were hold at this temperature for 2 minutes then stored separately in LN2. After 7 days of storage, straws from each freezing mode group were thawed and assessed for ACP and AKP in seminal plasma. In vivo fertility trials were also conducted with straws of control (fresh diluted semen) as well as freezing mode groups (frozen at different freezing rates). Least square analysis of variance for the data obtained revealed highly significant (P ≤ 0.01) rise in the seminal plasma ACP and AKP enzyme levels in frozen thawed semen as compared to that in fresh diluted semen. The Values of ACP and AKP also differed significantly (P ≤ 0.05) among all the freezing mode groups wherein lowest values of ACP were observed in M3 group followed by M4, M2 and M1 groups in increasing order whereas, lowest values of AKP were observed in M3 followed by M2, M4 and M1 groups in increasing order. Highest fertility rates were observed with semen from M3 followed by M2, M4 and M1 groups. On the basis of enzyme leakage and in-vivo fertility trials, the optimum freezing rate for cryopreservation protocol was arrived at 25° C/minute.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.8913 Int J Appl Sci Biotechnol, Vol. 1(4): 202-207
Effect of Hydropriming on Field Establishment of Seedlings obtained from Primed Seeds of Boro Rice cv. Brri Dhan29
Authors: Dey A. , Sarkar M. A. R. , Paul S. K. , Roy P. K. 
Published: December 2013

An experiment was conducted at the Seed Laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from January to April 2012 to study the effect of hydropriming on field establishment of seedlings obtained from primed seeds of Boro rice cv. BRRI dhan29. Seeds were soaked in water for 0, 24, 30, 36, 42, 48, 54 and 60 hours. The incubation period was 30 hours at 35°C temperature. Seed quality viz. percent germination, mean germination time, vigor index, shoot length, root length, shoot dry weight and root dry weight of rice seedlings were measured. Plant population m-2 also recorded to understand the field establishment of primed seeds. It was observed that priming treatments had significant effect on germination and other growth parameters of rice seedlings. The highest germination, vigor index, population m-2, length of shoot and root and their weight were found at 15 and 30 DAS. The lowest mean germination time was observed from hydropriming of seeds with 30 hours soaking. On the contrary, no priming treatment showed the lowest germination, vigor index, population m-2, and the highest mean germination time. The study concludes that BRRI dhan29 rice seed could be primed for 30 hours as hydropriming improves germination and field establishment of rice seedlings of Boro rice cv. BRRI dhan29.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9102 Int J Appl Sci Biotechnol, Vol. 1(4): 220-223
Toxicity of Dietary Hibiscus sabdariffa Linn (Malvaceae) Seed to Wistar Rats
Authors: Al Bahi Amgad A. H. , Abdelgadir Warda S. 
Published: December 2013

In Sudan Hibiscus sabdariffa seeds after fermentation are commonly known as Furundu with high quality proteins consumed as substitute to meat mainly in the Western part of the country. The raw seed, 10, 15 and 20 % of the standard diet was fed to Wister rats for periods up to 12 weeks. These doses were found to be non-toxic but not lethal to the treated rats. There was no evidence of carcinogenic effect of Hibiscus sabdariffa seed on any of the vital organs, but the main features were depression of growth and diverse pathological changes on the liver, kidneys and intestines which were correlated with highly significant (P<0.001) elevation of serum Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) and Alkaline phosphatase (ALP) activities and bilirubin (P<0.01) concentration in addition to a significant decrease (P<0.01) in cholesterol concentration. No change was observed in the total protein, globulin and urea concentrations of the test groups and albumin concentration in Groups 1 and 2 fed on diet containing 10 and 15% H. sabdarrifa seed. These findings were accompanied by leukocytosis and significantly lower red blood cells and haemoglobin concentration. Due to the elevated Mean corpuscular volume (MCV) and the normal values of the mean corpuscular Haemoglobin concentration (MCHC) in all the treatment groups the toxicity of H. Sabdariffa seed to Wistar rats resulted in macrocytic normochromic type of anaemia.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9103 Int J Appl Sci Biotechnol, Vol. 1(4): 214-219
Effect of Poly (N-IsoPropylAcrylamide) “PNIPAM” on Hepatic Cells of Swiss Albino Mice, Mus musculus
Authors: Aslam Md. Imtiyaz , Verma Rohit Kumar , Roy Richa , Roy S. P. 
Published: December 2013

The present paper deals with the effect of polymeric compound poly (N-isopropyl acrylamide) “PNIPAM” for their toxicity on hepatic cells. The nanoparticle is a xenobiotic compound that accumulates in the liver for their metabolism. Non-metabolizing xenobiotic compounds such as PNIPAM produces anomalies in the hepatic cells. Certain enzymes such as ALT and ALP can be assayed for the hepatocytic toxicity.An attempt has been made to know the toxic effect of “PNIPAM” in a concentration of 0.8mg/ml on the hepatic cells of Swiss Albino mice, Mus musculus. The ALT and ALP analysis were performed through test kits for their quantitative estimation. The histological result shows that several lesions were produced after the introduction of aqueous solution of PNIPAM for an incubation period of 48 hours. The toxicity was confirmed Spectrophotometrically by the assessment of enzyme ALT and ALP. The increased concentration of ALT (55.0 IU/L) and slight decrease in ALP (40.0 IU/L) concentration was responsible for the metabolic alteration and production of hepatocytic anomalies in the mice.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9104 Int J Appl Sci Biotechnol, Vol. 1(4): 208-213
Mitigation of the Adverse Effects of Salt Stress on Maize (Zea Mays L.) Through Organic Amendments
Authors: Das Debasish Kumar , Dey Bijaya Rani , Mian M.Joinul Abedin , Hoque Md. Anamul 
Published: December 2013

Salinity is a major limiting factor for crop production in coastal areas of Bangladesh. Organic amendments could contribute to the improvement of crop production in coastal areas. Two maize cultivars (BARI Hybrid Maize-5 and Hybrid Maize Pacific-987) were grown in pots to investigate the mitigating adverse effects of salt stress in maize by organic amendments. Two doses of farmyard manure (FYM) and poultry manure (PM) were mixed with soils before seed sowing. Plants were subjected to salinity (0-50 mM NaCl) at vegetative stage. Salt stress caused a significant reduction in growth and yield of both maize cultivars. Higher NaCl (50 mM) stress caused a drastic decrease in growth and yield of both maize cultivars. Salinity also decreased reproductive growth, chlorophyll contents and K+/Na+ ratio in both maize cultivars. Organic amendments with FYM and PM improved salt tolerances of maize that were associated with increased yield components, chlorophyll content and K+/Na+ ratio. Hybrid Maize Pacific-987 grown in low salinity with FYM or PM amendments produced higher yield than control condition. On the contrary, BARI Hybrid Maize-5 conferred tolerance to high salinity, when soils were amended with FYM or PM. Furthermore, organic amendments improved electrical conductivity, exchangeable Na and organic matter status under salinity condition. The present study suggests that organic amendments with FYM or PM confer tolerance to salinity in maize by increasing chlorophyll content and K+/Na+ ratio.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9128 Int J Appl Sci Biotechnol, Vol. 1(4): 233-239
Effect of Osmopriming of Boro Rice Seeds on Field Establishment of Seedlings
Authors: Roy P. K. , Sarkar M. A. R. , Paul S. K. , Dey A. 
Published: December 2013

An experiment was conducted at the Seed Laboratory of the Department of Agronomy, Bangladesh Agricultural University, Mymensingh during the period from January to April 2012 to study the effect of osmopriming of Boro rice seed (cv. BRRI dhan29). Seeds were soaked in 1%, 3% and 5% solutions of ZnSO4, KCl and CaCl2 for 24 hours, respectively, with 30 hours incubation at 35°C temperature. Seed quality tests such as percent germination, mean germination time, vigor index, shoot length, root length, shoot dry weight and root dry weight of rice seedlings were measured for all treatments. Population m-2 was also calculated to investigate the field establishment of primed seeds. The highest germination, vigor index, population m-2, shoot length and shoot dry weight at 15 and 30 DAS, root length at 15 DAS, root dry weight at 15 and 30 DAS and the lowest mean germination time was observed from priming of seeds with 3% ZnSO4. Exceptionally, priming with 3% CaCl2 showed the highest root length at 30 DAS. On the contrary, 3% and 5% CaCl2 priming treatment showed the lowest germination, vigor index, population m-2, shoot length and shoot dry weight at 15 and 30 DAS, root length at 15 DAS, root dry weight at 15 and 30 DAS and the highest mean germination time. It can be concluded that rice seed (cv. BRRI dhan29) can be primed with 3% ZnSO4 solution for improving germination and field establishment of rice seedlings.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9131 Int J Appl Sci Biotechnol, Vol. 1(4): 228-232
Seroprevalence 0f Dengue Virus Infection in Nepal
Authors: Gupta B P , Mishra S K , Manandhar K D , Malla R , Tamarakar C S , Raut P P , Sah S K , Pokhrel S , Rauniyar R , Bajaracharya A. 
Published: December 2013

Dengue Virus infection is an emerging mosquito-borne disease. It is a global health problem and its expanding endemicity towards new territories is a serious concern. Relatively a new disease in Nepalese context, dengue abruptly appeared as massive outbreak in 2010, merely four years after its first introduction. It is a nagging public health problem in the low lands of Terai, expanding to new areas of Nepal in recent years. A cross-sectional study was conducted to determine anti-Dengue IgM positive rate in Lumbini, Dhading and Chitwan district. The study was carried from June 2012 to November 2012. The total number of Serum samples was collected from 275 patients visiting hospitals with history of fever, headache and suspected DF. The samples were examined by ELISA. The anti-Dengue IgM positivity was found to be 29.09 %. The positive rate was highest in Dhading (70.37%) followed by Bharatpur (37.6%) and Lumbini (11.38%). The Dengue positive cases were higher in males (32.5 %) than female (24.8 %). The highest positive cases (41.6%) were from age group less than 15 years. Dengue has substantial expansion in Western and Far Western Terai region of Nepal which was limited to the middle Terai region in the past and mostly infects older people.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9135 Int J Appl Sci Biotechnol, Vol. 1(4): 224-227
Decolourization of Orange G Dye by Microalgae Acutodesmus obliquues Strain PSV2 Isolated from Textile Industrial Site
Authors: Sarwa Prakash , Verma Sanjay Kumar 
Published: December 2013

Introduction-Release of different types of synthetic dyes into the aquatic environment by various industrial sources is a major area of concern worldwide due to their low biodegradable nature. Conventional methods of dye removal are effective but high operating cost and energy requirement, generation of toxic sludge and regeneration problem of adsorbent limits their use at large scale. Biosorption of dye molecules by microorganisms is an economic and eco friendly technology for the treatment of textile wastewater. Aims & objective-The study was carried out to isolate microalgae from industrial site and to evaluate its efficiency for adsorption of Orange G dye from aqueous solution. Materials and methods- A unicellular green micro alga was isolated from textile and dye contaminated site of Sanganer, Jaipur (India) .Biosorption studies were conducted in a batch system for the removal of orange G dye from aqueous solution. Different parameters of pH, time, and initial dye concentration were investigated to optimize the process. Kinetic and equilibrium isotherms were implemented to experimental data in order to investigate the mechanism of biosorption. Results- Microalgae isolated from industrial site was found to be highly efficient for absorption of Orange G dye from aqueous solution. Batch studies depicts that Acutodesmusobliquues strain PSV 2 can absorb orange G dye at optimum pH 2.0 and maximum absorption (56.49 mg/g) occurred within initial 60 min of contact time. Equilibrium studies showed that Freundlich isotherm fitted well with experimental data with high correlation coefficient (r2=0.995) and n value (n=1.192). Langmuir isotherm showed r2=0.96 and value of b=0.008, which represents favorable adsorption of dye on algal surface. FTIR analysis of adsorbent before and after dye adsorption showed the involvement of some functional groups in dye absorption. DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9141 Int J Appl Sci Biotechnol, Vol. 1(4): 247-252
Quality of the Food and Diet Composition in Indian Bison (Bosgaurus) Based On Fecal Analysis in Mookambika Wild Life Sanctuary, Karnataka
Authors: Prashanth P.K. M. , Kumara Vijaya , Thirumala S. 
Published: December 2013

Quality of food and Diet composition of the Indian Bison (Bosgaurus) was estimated by fecal analysis. The results, together with studies in other parts of India, indicate that gaurs are primarily intermediate or adaptable mixed feeders. Fecal composition varied seasonally, with high proportion of grasses, forbs, and woody plant leaves, particularly Cynodondactylon, Cyperusrotundusin monsoon and post monsoon, and Strobilanthescallosus, Strobilanthesixiocephalus, Grewiatiliaefoliaand Syzygiumcuminiin winter and summer. Gaur selected herbs, shrubs, and grasses, and avoided eating woody plants for most of the year. Seasonal changes in the chemical composition of the feces were related to changes in phenology. The levels of crude protein, within certain limitations, and lignin in the feces were probably the most reliable indicators of diet quality. The ratio of crude protein: lignin was highest in monsoon and winter, corresponding early growing and fruiting seasons respectively. The usefulness of feces in estimating the composition and quality of the diet of an intermediate feeder is assessed.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9147 Int J Appl Sci Biotechnol, Vol. 1(4): 240-246
Anti epileptic activity of ocimum species: A brief review
Authors: Agarwal Chhaya , Sharma N. L. , Gaurav S. S. 
Published: December 2013

The Ocimum species is a medicinal herb used in the indigenous system of medicine. Ocimum sp. have variety of biological, pharmacological properties such as antibacterial, antiviral, antifungal, antimalarial, anthelmentic, antidiarrhoeal, antiinflammatory, antihypertensive, cardioprotective, central nervous system (CNS) depressant, antidiabetic, antithyroidic, antioxidant, anticancer, chemopreventive, radioprotective, immunomodulatory, antifertility, antiulcer, antiarthritic, antistress, antileucodermal and anticoagulant activities. Sevral species of Ocimum are used to cure central nervous system (CNS) disorders.in various part of the world due to its anticonvulsant property .epilepsy is a chronic disorder which is characterized by seizures. Seizures are resistant to treatment with currently available anticonvulsant drug (AEDs) in about one out of three patient with epilepsy. This review refers to the study of ocimum as an antiepileptic drug (AEDs) because of its specific anticonvulsant property.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9168 Int J Appl Sci Biotechnol, Vol. 1(4): 180-183
Effect of Date of Sowing on the Performance of Drought Tolerant Wheat Genotypes to Spot Blotch at Rampur, Chitwan , Nepal
Authors: Aryal L. , Shrestha S. M. , K.C. G. B. 
Published: December 2013

A research was conducted in field to elucidate the response of 20 twenty drought tolerant wheat genotypes to spot blotch. Field experiment was carried out in Agronomy block of IAAS, Rampur, Chitwan in split plot design with three replications, considering 25 November as normal sowing and 15 December as late sowing dates taking them as main factors and genotypes as sub factors. Grain yield was 2.50 t ha-1 on 25 November sowing and 2.03 t ha-1 on 15 December sowing. Similarly thousand kernel weight was 37.50 g on 25 November sowing and 32.82 g on 15 December sowing. Aditya, CSISA DRYT 5204 and CSISA DRYT 5205 had less than 13% grain yield and TKW reduction when sown in late condition also. From the experiment it is concluded that these three genotypes can be sown in late condition as they were resistant to spot blotch and heat stress.DOI: http://dx.doi.org/10.3126/ijasbt.v1i4.9180 Int J Appl Sci Biotechnol, Vol. 1(4): 266-271
Breast cancer and circadian disruption from electric lighting in the modern world
Authors: Stevens Richard G. , Brainard George C. , Blask David E. , Lockley Steven W. , Motta Mario E. 
Published: December 2013

AbstractBreast cancer is the leading cause of cancer death among women worldwide, and there is only a limited explanation of why. Risk is highest in the most industrialized countries but also is rising rapidly in the developing world. Known risk factors account for only a portion of the incidence in the high‐risk populations, and there has been considerable speculation and many false leads on other possibly major determinants of risk, such as dietary fat. A hallmark of industrialization is the increasing use of electricity to light the night, both within the home and without. It has only recently become clear that this evolutionarily new and, thereby, unnatural exposure can disrupt human circadian rhythmicity, of which three salient features are melatonin production, sleep, and the circadian clock. A convergence of research in cells, rodents, and humans suggests that the health consequences of circadian disruption may be substantial. An innovative experimental model has shown that light at night markedly increases the growth of human breast cancer xenografts in rats. In humans, the theory that light exposure at night increases breast cancer risk leads to specific predictions that are being tested epidemiologically: evidence has accumulated on risk in shift workers, risk in blind women, and the impact of sleep duration on risk. If electric light at night does explain a portion of the breast cancer burden, then there are practical interventions that can be implemented, including more selective use of light and the adoption of recent advances in lighting technology and application. CA Cancer J Clin 2014;64:207–218. © 2013 American Cancer Society.
STAT5-Induced Lunatic Fringe during Th2 Development Alters Delta-like 4–Mediated Th2 Cytokine Production in Respiratory Syncytial Virus–Exacerbated Airway Allergic Disease
Authors: Mukherjee Sumanta , Rasky Andrew J. , Lundy Phil A. , Kittan Nicolai A. , Kunkel Steven L. , Maillard Ivan P. , Kowalski Paul E. , Kousis Philaretos C. , Guidos Cynthia J. , Lukacs Nicholas W. 
Published: December 2013

Abstract
Notch activation plays an important role in T cell development and mature T cell differentiation. In this study, we investigated the role of Notch activation in a mouse model of respiratory syncytial virus (RSV)–exacerbated allergic airway disease. During RSV exacerbation, in vivo neutralization of a specific Notch ligand, Delta-like ligand (Dll)-4, significantly decreased airway hyperreactivity, mucus production, and Th2 cytokines. Lunatic Fringe (Lfng), a glycosyltransferase that enhances Notch activation by Dll4, was increased during RSV exacerbation. Lfng loss of function in Th2-skewed cells inhibited Dll4-Notch activation and subsequent IL-4 production. Further knockdown of Lfng in T cells in CD4Cre+Lfngfl/fl mice showed reduced Th2 response and disease pathology during RSV exacerbation. Finally, we identified STAT5-binding cis-acting regulatory element activation as a critical driver of Lfng transcriptional activation. These data demonstrate that STAT5-dependent amplification of Notch-modifying Lfng augments Th2 response via Dll4 and is critical for amplifying viral exacerbation during allergic airway disease.
Bach2 Regulates Homeostasis of Foxp3+ Regulatory T Cells and Protects against Fatal Lung Disease in Mice
Authors: Kim Eui Ho , Gasper David J. , Lee Song Hee , Plisch Erin Hemmila , Svaren John , Suresh M. 
Published: December 2013

Abstract
Variants of the Bach2 gene are linked to vitiligo, celiac disease, and type 1 diabetes, but the underlying immunological mechanisms are unknown. In this study, we demonstrate that Bach2 plays crucial roles in maintaining T cell quiescence and governing the differentiation, activation, and survival of Foxp3+ regulatory T (Treg) cells. Bach2-deficient T cells display spontaneous activation and produce elevated levels of Th1/Th2-type cytokines. Without Bach2, Treg cells exhibit diminished Foxp3 expression, depleted numbers, hyperactivation, enhanced proliferation, and profound loss of competitive fitness in vivo. Mechanistically, reduced survival of Bach2-deficient Treg cells was associated with reduced Bcl-2 and Mcl-1 levels and elevated Bim/Bcl-2 ratio. Additionally, Bach2 deficiency induced selective loss of Helios−Foxp3+ Treg cells and a Treg cell transcriptome skewed toward the Th1/Th2 effector program at the expense of the Treg program. In vitro experiments confirmed that Bach2: 1) is indispensable for TCR/TGF-β–induced Foxp3 expression; and 2) mitigates aberrant differentiation of Treg cells by repression of the competing Gata3-driven Th2 effector program. Importantly, perturbations in the differentiation of induced Treg cells was linked to a fatal Th2-type chronic inflammatory lung disease in Bach2-deficient mice. Thus, Bach2 enforces T cell quiescence, promotes the development and survival of Treg lineage, restrains aberrant differentiation of Treg cells, and protects against immune-mediated diseases.