bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential role of p21Waf1/Cip1 in the modulation of post-traumatic hippocampal Neural Stem Cells response
Authors: Chiani Francesco , Mastrorilli Valentina , Marchetti Nicole , Macioce Andrea , Nappi Chiara , Strimpakos Georgios , Pasquini Miriam , Gambadoro Alessia , Battistini Jonathan Isacco , Cutuli Debora , Petrosini Laura , Marinelli Sara , Scardigli Raffaella , Farioli Vecchioli Stefano 
Published: July 2024

Abstract
Background
Traumatic Brain Injury (TBI) represents one of the main causes of brain damage in young people and the elderly population with a very high rate of psycho-physical disability and death. TBI is characterized by extensive cell death, tissue damage and neuro-inflammation with a symptomatology that varies depending on the severity of the trauma from memory loss to a state of irreversible coma and death. Recently, preclinical studies on mouse models have demonstrated that the post-traumatic adult Neural Stem/Progenitor cells response could represent an excellent model to shed light on the neuro-reparative role of adult neurogenesis following damage. The cyclin-dependent kinase inhibitor p21Waf1/Cip1 plays a pivotal role in modulating the quiescence/activation balance of adult Neural Stem Cells (aNSCs) and in restraining the proliferation progression of progenitor cells. Based on these considerations, the aim of this work is to evaluate how the conditional ablation of p21Waf1/Cip1 in the aNSCS can alter the adult hippocampal neurogenesis in physiological and post-traumatic conditions.

Methods
We designed a novel conditional p21Waf1/Cip1 knock-out mouse model, in which the deletion of p21Waf1/Cip1 (referred as p21) is temporally controlled and occurs in Nestin-positive aNSCs, following administration of Tamoxifen. This mouse model (referred as p21 cKO mice) was subjected to Controlled Cortical Impact to analyze how the deletion of p21 could influence the post-traumatic neurogenic response within the hippocampal niche.

Results
The data demonstrates that the conditional deletion of p21 in the aNSCs induces a strong increase in activation of aNSCs as well as proliferation and differentiation of neural progenitors in the adult dentate gyrus of the hippocampus, resulting in an enhancement of neurogenesis and the hippocampal-dependent working memory. However, following traumatic brain injury, the increased neurogenic response of aNSCs in p21 cKO mice leads to a fast depletion of the aNSCs pool, followed by declined neurogenesis and impaired hippocampal functionality.

Conclusions
These data demonstrate for the first time a fundamental role of p21 in modulating the post-traumatic hippocampal neurogenic response, by the regulation of the proliferative and differentiative steps of aNSCs/progenitor populations after brain damage.
Decoding cellular plasticity and niche regulation of limbal stem cells during corneal wound healing
Authors: Sun Di , Zhang Xiaowen , Chen Rong , Sang Tian , Li Ya , Wang Qun , Xie Lixin , Zhou Qingjun , Dou Shengqian 
Published: July 2024

Abstract
Background
Dysfunction or deficiency of corneal epithelium results in vision impairment or blindness in severe cases. The rapid and effective regeneration of corneal epithelial cells relies on the limbal stem cells (LSCs). However, the molecular and functional responses of LSCs and their niche cells to injury remain elusive.

Methods
Single-cell RNA sequencing was performed on corneal tissues from normal mice and corneal epithelium defect models. Bioinformatics analysis was performed to confirm the distinct characteristics and cell fates of LSCs. Knockdown of Creb5 and OSM treatment experiment were performed to determine their roles of in corneal epithelial wound healing.

Results
Our data defined the molecular signatures of LSCs and reconstructed the pseudotime trajectory of corneal epithelial cells. Gene network analyses characterized transcriptional landmarks that potentially regulate LSC dynamics, and identified a transcription factor Creb5, that was expressed in LSCs and significantly upregulated after injury. Loss-of-function experiments revealed that silencing Creb5 delayed the corneal epithelial healing and LSC mobilization. Through cell–cell communication analysis, we identified 609 candidate regeneration-associated ligand-receptor interaction pairs between LSCs and distinct niche cells, and discovered a unique subset of Arg1+ macrophages infiltrated after injury, which were present as the source of Oncostatin M (OSM), an IL-6 family cytokine, that were demonstrated to effectively accelerate the corneal epithelial wound healing.

Conclusions
This research provides a valuable single-cell resource and reference for the discovery of mechanisms and potential clinical interventions aimed at ocular surface reconstruction.
Soft information acceleration aided subspace suppression MIMO detection
Authors: Xiong XiaoXiong , Dong Chao , Bian Yaohui , Niu Kai 
Published: July 2024

AbstractIn this paper, a multiple-input multiple-output detection structure called soft information acceleration (SIA) is proposed, which is suitable for simplifying the two-stage subspace marginalization with interference suppression (SUMIS) into one stage. The proposed one-stage method outperforms the conventional two-stage SUMIS when the subspace size is large enough. The performance advantage of the proposed SUMIS-SIA mainly results from the average number of soft information updates being equal to the ’subspace size,’ instead of only once during the two-stage SUMIS detection. Thus, the SUMIS-SIA achieves a better trade-off between performance and complexity. To further reduce the complexity, a channel-shortening method based on subspace suppression is proposed. Simulation results show that the proposed channel-shortening one-stage method also outperforms SUMIS, which benefits from SIA.
Association of preserved ratio impaired spirometry with mortality and cardiovascular diseases: a systematic review and meta-analysis
Authors: Li Mengya , Chen Mengxin , Li Yang , Liu Zhiguang , Li Xiaocong , Lang Xinyue , Hu Bo , Li Wei 
Published: July 2024

Abstract
Background
Preserved ratio impaired spirometry (PRISm) is a type of abnormal lung function. PRISm and mortality have been explored in several studies, but a comprehensive evaluation of the associations is limited. The current study aims to conduct a systematic review and meta-analysis in order to investigate the mortality and cardiovascular diseases in patients with PRISm.

Methods
PubMed, Embase, and Web of Science databases, as well as gray literature sources, were searched for relevant studies published up to 7 September 2023 without language restrictions. This review included all published observational cohort studies that investigated the association of PRISm with mortality in the general population, as well as subgroup analyses in smokers and pre-bronchodilation spirometry studies. The outcomes of interest were all-cause mortality, cardiovascular mortality, and respiratory-related mortality. The Newcastle–Ottawa scale assessed study quality. Sensitivity and subgroup analyses explored heterogeneity and robustness. Publication bias was assessed with Egger’s and Begg’s tests.

Results
Overall, eight studies were included in this meta-analysis. The pooled HR was 1.60 (95% CI, 1.48–1.74) for all-cause mortality, 1.68 (95% CI, 1.46–1.94) for CVD mortality, and 3.09 (95% CI, 1.42–6.71) for respiratory-related mortality in PRISm group compared to normal group. In the subgroup analysis, participants with PRISm had a higher effect (HR, 2.11; 95% CI, 1.74–2.54) on all-cause mortality among smokers relative to participants with normal spirometry. Furthermore, the association between PRISm and mortality risk was consistent across several sensitivity analyses.

Conclusions
People with PRISm were associated with an increased risk of all-cause mortality, CVD mortality, and respiratory-related mortality as compared to those with normal lung function in the general population.

Systematic review registration
PROSPERO CRD42023426872.
Impact of the COVID-19 pandemic on extended-spectrum β-lactamase producing Escherichia coli in urinary tract and blood stream infections: results from a nationwide surveillance network, Finland, 2018 to 2022
Authors: Ilmavirta Heikki , Ollgren Jukka , Räisänen Kati , Kinnunen Tuure , Hakanen Antti Juhani , Rantakokko-Jalava Kaisu , Jalava Jari , Lyytikäinen Outi 
Published: July 2024

Abstract
Background
Before the COVID-19 pandemic there has been a constant increase in antimicrobial resistance (AMR) of Escherichia coli, the most common cause of urinary tract infections and bloodstream infections. The aim of this study was to investigate the impact of the COVID-19 pandemic on extended-spectrum β-lactamase (ESBL) production in urine and blood E. coli isolates in Finland to improve our understanding on the source attribution of this major multidrug-resistant pathogen.

Methods
Susceptibility test results of 564,233 urine (88.3% from females) and 23,860 blood E. coli isolates (58.8% from females) were obtained from the nationwide surveillance database of Finnish clinical microbiology laboratories. Susceptibility testing was performed according to EUCAST guidelines. We compared ESBL-producing E. coli proportions and incidence before (2018–2019), during (2020–2021), and after (2022) the pandemic and stratified these by age groups and sex.

Results
The annual number of urine E. coli isolates tested for antimicrobial susceptibility decreased 23.3% during 2018–2022 whereas the number of blood E. coli isolates increased 1.1%. The annual proportion of ESBL-producing E. coli in urine E. coli isolates decreased 28.7% among males, from 6.9% (average during 2018–2019) to 4.9% in 2022, and 28.7% among females, from 3.0 to 2.1%. In blood E. coli isolates, the proportion decreased 32.9% among males, from 9.3 to 6.2%, and 26.6% among females, from 6.2 to 4.6%. A significant decreasing trend was also observed in most age groups, but risk remained highest among persons aged ≥ 60 years.

Conclusions
The reduction in the proportions of ESBL-producing E. coli was comprehensive, covering both specimen types, both sexes, and all age groups, showing that the continuously increasing trends could be reversed. Decrease in international travel and antimicrobial use were likely behind this reduction, suggesting that informing travellers about the risk of multidrug-resistant bacteria, hygiene measures, and appropriate antimicrobial use is crucial in prevention. Evaluation of infection control measures in healthcare settings could be beneficial, especially in long-term care.
Source and level of dietary iron influence semen quality by affecting inflammation, oxidative stress and iron utilization levels in boars
Authors: Wu Yinghui , Li Yamei , Miao Yueyue , Wei Hongkui , Luo Hefeng , Ren Chunxiao , Zhang Yawei , Chen Juan , Wei Tanghong , Deng Jiyan , Peng Jian 
Published: July 2024

Abstract
Background
Boars fed a mixed form of inorganic and organic iron in excess of the NRC recommended levels still develop anemia, which suggested that the current level and form of iron supplementation in boar diets may be inappropriate. Therefore, 56 healthy Topeka E line boars aged 15–21 months were randomly divided into 5 groups: basal diet supplemented with 96 mg/kg ferrous sulfate (FeSO4) and 54 mg/kg glycine chelated iron (Gly-Fe, control); 80 mg/kg or 115 mg/kg Gly-Fe; 80 mg/kg or 115 mg/kg methionine hydroxyl analogue chelated iron (MHA-Fe, from Calimet-Fe) for 16 weeks. The effects of dietary iron supplementation with different sources and levels on semen quality in boars were investigated.

Results
1) Serum Fe and hemoglobin concentrations were not affected by reduced dietary iron levels in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups compared with the control group (P > 0.05). 2) Serum interleukin-6 (IL-6) and sperm malondialdehyde (MDA) levels in the 80 mg/kg or 115 mg/kg MHA-Fe groups were lower than those in the control group (P < 0.05), and higher serum superoxide dismutase levels and lower MDA levels in the 115 mg/kg MHA-Fe group (P < 0.05). 3) Boars in the 80 mg/kg or 115 mg/kg Gly-Fe and MHA-Fe groups had lower serum hepcidin (P < 0.01), ferritin (P < 0.05), and transferrin receptor (P < 0.01) concentrations, and boars in the 115 mg/kg MHA-Fe group had higher seminal plasma Fe concentrations compared with the control group. 4) Boars in the 80 mg/kg and 115 mg/kg MHA-Fe groups had lower abnormal sperm rate and in situ oscillating sperm ratio compared to the control group at weeks 12 and/or 16 of the trial. However, the effect of Gly-Fe on improving semen quality in boars was not evident. 5) Serum IL-6 level was positively correlated with hepcidin concentration (P < 0.05), which in turn was significantly positively correlated with abnormal sperm rate (P < 0.05). Furthermore, significant correlations were also found between indicators of iron status and oxidative stress and semen quality parameters.

Conclusions
Dietary supplementation with 80 mg/kg or 115 mg/kg MHA-Fe did not induce iron deficiency, but rather reduced serum inflammatory levels and hepcidin concentration, alleviated oxidative stress, increased body iron utilization, and improved semen quality in adult boars.
Land use evaluation and capacity assessment for sustainable urban physical development: case of study Ahvaz city
Authors: Shamai Ali , Jafarpour Ghalehteimouri Kamran 
Published: July 2024

AbstractLand use evaluation and capacity assessment for sustainable urban development in Ahvaz city has become crucial due to rapid urban expansion negatively impacting current and future development. Urban population growth and resource demands have led to a decline in environmental quality. Over the past decades, Ahvaz has identified potential areas for future expansion until 2030. This study assesses the city's physical expansion and spatial development patterns, aiming to identify optimistic future development scenarios. Emphasizing balanced and equitable development, the study focuses on suitable areas, environmental preservation, pollution reduction, and mitigating urban hazards . Various factors, including land use, soil type, vegetation density, landform, groundwater level, geology, and distances from railways, industries, roads, hinterland cities, water networks, faults, and the city itself, are considered. Three decision-making methods—logic, Analytical Hierarchy Process (AHP), and integrated Fuzzy-AHP—are applied to map urban physical development. Fuzzy logic, AHP models, Expert Choice, Arc GIS software, and a land fit zoning map are used for data analysis. The AHP assesses the factors' relative importance, followed by standardization based on fuzzy set theory. Criteria factors are then combined using AHP and fuzzy logic to generate a land suitability map. The study identifies five zones for Ahvaz's future development—very high, high, medium, low, and very low—based on their physical expansion potential. The third scenario, supported by maps, indicates favorable conditions for future development in the northwest and southwest zones, considering environmental characteristics, infrastructure, and land continuity.
Fishers (Pekania pennanti) are forest structure specialists when resting and generalists when moving: behavior influences resource selection in a northern Rocky Mountain fisher population
Authors: Olson Lucretia E. , Sauder Joel D. , Fekety Patrick A. , Golding Jessie D. , Lewis Carly W. , Sadak Rema B. , Schwartz Michael K. 
Published: July 2024

Abstract
Background
Studies of animal habitat selection are important to identify and preserve the resources species depend on, yet often little attention is paid to how habitat needs vary depending on behavioral state. Fishers (Pekania pennanti) are known to be dependent on large, mature trees for resting and denning, but less is known about their habitat use when foraging or moving within a home range.

Methods
We used GPS locations collected during the energetically costly pre-denning season from 12 female fishers to determine fisher habitat selection during two critical behavioral activities: foraging (moving) or resting, with a focus on response to forest structure related to past forest management actions since this is a primary driver of fisher habitat configuration. We characterized behavior based on high-resolution GPS and collar accelerometer data and modeled fisher selection for these two behaviors within a home range (third-order selection). Additionally, we investigated whether fisher use of elements of forest structure or other important environmental characteristics changed as their availability changed, i.e., a functional response, for each behavior type.

Results
We found that fishers exhibited specialist selection when resting and generalist selection when moving, with resting habitat characterized by riparian drainages with dense canopy cover and moving habitat primarily influenced by the presence of mesic montane mixed conifer forest. Fishers were more tolerant of forest openings and other early succession elements when moving than resting.

Conclusions
Our results emphasize the importance of considering the differing habitat needs of animals based on their movement behavior when performing habitat selection analyses. We found that resting fishers are more specialist in their habitat needs, while foraging fishers are more generalist and will tolerate greater forest heterogeneity from past disturbance.
A multi-channel stimulator with an active electrode array implant for vagal-cardiac neuromodulation studies
Authors: Liu Fangqi , Habibollahi Maryam , Wu Yu , Neshatvar Nazanin , Zhang Jiaxing , Zinno Ciro , Akouissi Outman , Bernini Fabio , Alibrandi Lisa , Gabisonia Khatia , Lionetti Vincenzo , Carpaneto Jacopo , Lancashire Henry , Jiang Dai , Micera Silvestro , Demosthenous Andreas 
Published: July 2024

Abstract
Background
Implantable vagus nerve stimulation is a promising approach for restoring autonomic cardiovascular functions after heart transplantation. For successful treatment a system should have multiple electrodes to deliver precise stimulation and complex neuromodulation patterns.

Methods
This paper presents an implantable multi-channel stimulation system for vagal-cardiac neuromodulation studies in swine species. The system comprises an active electrode array implant percutaneously connected to an external wearable controller. The active electrode array implant has an integrated stimulator ASIC mounted on a ceramic substrate connected to an intraneural electrode array via micro-rivet bonding. The implant is silicone encapsulated for biocompatibility and implanted lifetime. The stimulation parameters are remotely transmitted via a Bluetooth telemetry link.

Results
The size of the encapsulated active electrode array implant is 8 mm × 10 mm × 3 mm. The stimulator ASIC has 10-bit current amplitude resolution and 16 independent output channels, each capable of delivering up to 550 µA stimulus current and a maximum voltage of 20 V. The active electrode array implant was subjected to in vitro accelerated lifetime testing at 70 °C for 7 days with no degradation in performance. After over 2 h continuous stimulation, the surface temperature change of the implant was less than 0.5 °C. In addition, in vivo testing on the sciatic nerve of a male Göttingen minipig demonstrated that the implant could effectively elicit an EMG response that grew progressively stronger on increasing the amplitude of the stimulation.

Conclusions
The multi-channel stimulator is suitable for long term implantation. It shows potential as a useful tool in vagal-cardiac neuromodulation studies in animal models for restoring autonomic cardiovascular functions after heart transplantation.
Assessment of airflow limitation in patients with obstructive sleep apnea
Authors: El-Sorougi Waleed Mohamed Kamal Eldin , Ali Mohamed Alaa Abdelhamid , Abdalrazik Fatmaalzahraa Saad 
Published: July 2024

Abstract
Background
Obstructive sleep apnea (OSA) is a prevalent sleep breathing disorder affecting 9–25% of the general adult population.

Aim
To assess airflow limitation by spirometric indices in patients with obstructive sleep apnea.

Patients and methods
This observational case–control study was conducted on 60 subjects who were divided into four groups: Group I (control group), included 20 subjects chosen from other departments, who had no respiratory complaints with apnea–hypopnea index (AHI < 5); Group II (mild group), included 11 patients with mild sleep apnea, 5 ≤ AHI < 15; Group III (moderate group), included 17 patients with moderate sleep apnea, 15 ≤ AHI < 30; and Group IV (severe group), included 12 patients with severe sleep apnea, AHI ≥ 30 at the Chest Department, Faculty of Medicine, Helwan University, from August 2021 until June 2022.

Results
There was no statistically significant relation found between the severity of AHI and all the previous pulmonary function parameters except a statistically significant decrease in FEF (25–75%) in the moderate group than the mild group and also in the severe group than the moderate group (p-value < 0.001). There was a statistically significant positive correlation found between AHI and BMI and NC and a negative correlation found between AHI and FEF (25–75%) while no statistically significant correlation was found between AHI and the other studied parameters.

Conclusion
Obstructive sleep apnea (OSA) is associated with airflow limitation by spirometric indices, although this association is statistically insignificant. On the other hand, the severity of obstructive sleep apnea is directly proportional to the seriousness of the apnea–hypopnea index (AHI). Strong correlations were found between the severity of AHI and body mass index (BMI), neck circumference, and FEF (25–75%).
Efficacy and Safety of Multikinase Inhibitors for Patients With Refractory Thyroid Cancer: Systematic Review and Network Meta-Analysis
Authors: Jing Ren , Wu Nan , Wu Yang , Zhang Qian , Liang Qiankun , Huang Peng , Yi Shijian 
Published: July 2024

Abstract

Context
Multikinase inhibitors (MKIs) improve the treatment of refractory thyroid cancer, including radioactive iodine-refractory differentiated thyroid cancer (RAIR-DTC) and advanced medullary thyroid carcinoma (aMTC).

Objective
This study aims to compare the efficacy of MKIs in improving survival outcomes and safety.

Data Sources
Comprehensive database searches of MEDLINE via PubMed, EMBASE, and Cochrane were performed from inception to December 2023.

Study Selection
Three independent authors selected these studies. Randomized controlled trials that compared the use of a MKI to other MKIs or placebo were included.

Data Extraction and Synthesis
This review followed Preferred Reporting Items for Systematic Reviews and Meta-Analysis guidelines. Risk of bias was analyzed using the Cochrane risk of bias 2 tool. Bayesian network meta-analysis was performed. Treatments were grouped into common nodes based on the type of MKI.

Main Outcomes and Measures
Primary outcomes were progression-free survival (PFS) and overall survival (OS). Secondary outcomes included objective response rate, disease control rate, clinical benefit rate, and adverse events.

Results
Cabozantinib 60 mg/day (CAB60) was associated with the highest prolonged PFS in RAIR-DTC patients, followed by lentivatinib 18 or 24 mg/day (LEN18 or LEN24), and apatinib. PFS was also improved in aMTC patients who received CAB 140 mg/day (CAB140), CAB60, or anlotinib. A significantly greater improvement on the performance of OS was seen in CAB60, LEN24, anlotinib, and sorafenib in RAIR-DTC patients, but in aMTC patients there were lack of statistical differences. Compared with the low-dose MKIs, high-dose MKIs such as CAB, LEN, and vandetanib increased the incidence of adverse events.

Conclusion
CAB60, LEN, and apatinib are promising topical MKIs with statistically significant primary outcomes in RAIR-DTC patients, while CAB and anlotinib are effective in prolonging PFS in aMTC patients.