bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantification of myo‐inositol, 1,5‐anhydro‐ D‐sorbitol, and D‐chiro‐inositol using high‐performance liquid chromatography with electrochemical detection in very small volume clinical samples
Authors: Schimpf Karen J. , Meek Claudia C. , Leff Richard D. , Phelps Dale L. , Schmitz Daniel J. , Cordle Christopher T. 
Published: May 2015

AbstractInositol is a six‐carbon sugar alcohol and is one of nine biologically significant isomers of hexahydroxycyclohexane. Myo‐inositol is the primary biologically active form and is present in higher concentrations in the fetus and newborn than in adults. It is currently being examined for the prevention of retinopathy of prematurity in newborn preterm infants. A robust method for quantifying myo‐inositol (MI), D‐chiro‐inositol (DCI) and 1,5‐anhydro‐ D‐sorbitol (ADS) in very small‐volume (25 μL) urine, blood serum and/or plasma samples was developed. Using a multiple‐column, multiple mobile phase liquid chromatographic system with electrochemical detection, the method was validated with respect to (a) selectivity, (b) accuracy/recovery, (c) precision/reproducibility, (d) sensitivity, (e) stability and (f) ruggedness. The standard curve was linear and ranged from 0.5 to 30 mg/L for each of the three analytes. Above‐mentioned performance measures were within acceptable limits described in the Food and Drug Administration's Guidance for Industry: Bioanalytical Method Validation. The method was validated using blood serum and plasma collected using four common anticoagulants, and also by quantifying the accuracy and sensitivity of MI measured in simulated urine samples recovered from preterm infant diaper systems. The method performs satisfactorily measuring the three most common inositol isomers on 25 μL clinical samples of serum, plasma, milk, and/or urine. Similar performance is seen testing larger volume samples of infant formulas and infant formula ingredients. MI, ADS and DCI may be accurately tested in urine samples collected from five different preterm infant diapers if the urine volume is greater than 2–5 mL. Copyright © 2015 John Wiley & Sons, Ltd.
Synthesis and in Vitro Antiviral Activities of [(Dihydrofuran‐2‐yl)oxy]methyl‐phosphonate Nucleosides with 2‐Substituted Adenine as Base
Authors: Liu Fei , Liu Yingju , Xu Rui‐Gang , Dai Guifu , Zhao Liu‐Xi , Wang Yafeng , Liu Hong‐Min , Liu Feng‐Wu , Pannecouque Christophe , Herdewijn Piet 
Published: May 2015

AbstractThe synthesis of [(2′,5′‐dihydrofuran‐2‐yl)oxy]methyl‐phosphonate nucleosides with a 2‐substituted adenine base moiety starting from 2‐deoxy‐3,5‐bis‐O‐(4‐methylbenzoyl)‐α‐L‐ribofuranosyl chloride and 2,6‐dichloropurine is described. The key step is the regiospecific and stereoselective introduction of a phosphonate synthon at C(2) of the furan ring. None of the synthesized compounds showed significant in vitro activity against HIV, BVDV, and HBV.
Interaction of β3/β2‐Peptides, Consisting of Val‐Ala‐Leu Segments, with POPC Giant Unilamellar Vesicles (GUVs) and White Blood Cancer Cells (U937) – A New Type of Cell‐Penetrating Peptides, and a Surprising Chain‐Length Dependence of Their Vesicle‐ and Cell‐Lysing Activity
Authors: Kolesinska Beata , Eyer Klaus , Robinson Tom , Dittrich Petra S. , Beck Albert K. , Seebach Dieter , Walde Peter 
Published: May 2015

AbstractMany years ago, β2/β3‐peptides, consisting of alternatively arranged β2‐ and β3h‐amino‐acid residues, have been found to undergo folding to a unique type of helix, the 10/12‐helix, and to exhibit non‐polar, lipophilic properties (Helv. Chim. Acta 1997, 80, 2033). We have now synthesized such ‘mixed’ hexa‐, nona‐, dodeca‐, and octadecapeptides, consisting of Val‐Ala‐Leu triads, with N‐terminal fluorescein (FAM) labels, i.e., 1–4, and studied their interactions with POPC (=1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphocholine) giant unilamellar vesicles (GUVs) and with human white blood cancer cells U937. The methods used were microfluidic technology, fluorescence correlation spectroscopy (FCS), a flow‐cytometry assay, a membrane‐toxicity assay with the dehydrogenase G6PDH as enzymatic reporter, and visual microscopy observations. All β3/β2‐peptide derivatives penetrate the GUVs and/or the cells. As shown with the isomeric β3/β2‐, β3‐, and β2‐nonamers, 2, 5, and 6, respectively, the derivatives 5 and 6 consisting exclusively of β3‐ or β2‐amino‐acid residues, respectively, interact neither with the vesicles nor with the cells. Depending on the method of investigation and on the pretreatment of the cells, the β3/β2‐nonamer and/or the β3/β2‐dodecamer derivative, 2 and/or 3, respectively, cause a surprising disintegration or lysis of the GUVs and cells, comparable with the action of tensides, viral fusion peptides, and host‐defense antimicrobial peptides. Possible sources of the chain‐length‐dependent destructive potential of the β3/β2‐nona‐ and β3/β2‐dodecapeptide derivatives, and a possible relationship with the phosphate‐to‐phosphate and hydrocarbon thicknesses of GUVs, and eukaryotic cells are discussed. Further investigations with other types of GUVs and of eukaryotic or prokaryotic cells will be necessary to elucidate the mechanism(s) of interaction of ‘mixed’ β3/β2‐peptides with membranes and to evaluate possible biomedical applications.
A 12–month angiographic and optical coherence tomography follow‐up after bioresorbable vascular scaffold implantation in patients with ST‐segment elevation myocardial infarction
Authors: Kochman Janusz , Tomaniak Mariusz , Kołtowski Łukasz , Jąkała Jacek , Proniewska Klaudia , Legutko Jacek , Roleder Tomasz , Pietrasik Arkadiusz , Rdzanek Adam , Kochman Wacław , Brugaletta Salvatore , Kaluza Grzegorz L. 
Published: May 2015

ObjectivesThe aim of the study was to evaluate the healing process at 12 months after ABSORB™ bioresorbable vascular scaffold (BVS) implantation in patients with ST‐segment elevation myocardial infarction (STEMI).BackgroundThere is currently no data on long‐term BVS performance in the acute thrombotic setting. The underlying altered plaque pathomorphology may impact the neointima healing pattern, potentially making it different to that observed in stable coronary artery disease (CAD).MethodsWe have performed an angiographic and optical coherence tomography (OCT) 12‐month follow‐up of 19 STEMI patients who were treated with a BVS implantation (23 scaffolds). An independent core laboratory performed a paired analysis of the corresponding frames at baseline and follow‐up.ResultsAt 12 months, the OCT follow‐up showed a decrease in the mean lumen area (8.29 ± 1.53 mm2 vs. 6.82 ± 1.57 mm2, P < 0.001), but no significant change in the mean scaffold area (8.49 ± 1.53 mm2 vs. 8.90 ± 1.51 mm2). Significant decreases in malapposed strut ratio (4.9 ± 8.65% vs. 0.4 ± 1.55%, P < 0.001) and malapposition area (0.29 ± 0.60 mm2 0.08 ± 0.32 mm2, P = 0.002) were observed. A nonhomogenous proliferation of neointima was revealed with a symmetry index of 0.15 (0.08–0.27), a mean neointima thickness of 203 μm (183–249) and mean neointima area of 2.07 ± 0.51 mm2. The quantitative coronary angiography showed late lumen loss of 0.08 ± 0.23 mm and no significant change in the minimal lumen diameter (P = 0.11). There were no major adverse cardiovascular events (MACE), except for one nontarget vessel revascularization.ConclusionsThe OCT revealed a favorable healing pattern after BVS implantation in a STEMI population. © 2015 Wiley Periodicals, Inc.
Bilateral femur metastases in low‐grade endometrial carcinoma
Authors: Lunardi Pierre , Vidal Fabien , Accadbled Franck , Leguevaque Pierre , Soule‐Tholy Marc , Beauval Jean Baptiste , Motton Stéphanie 
Published: May 2015

Key Clinical MessageLower‐limb bone metastases are uncommon in low‐grade endometrial carcinoma, and are mostly located on the axial skeleton. Here, we present a rare case of bilateral femur metastases in low‐grade endometrial carcinoma and performed a review of the current literature.
Nonsyndromic X‐linked intellectual deficiency in three brothers with a novel MED12 missense mutation [c.5922G>T (p.Glu1974His)]
Authors: Bouazzi Habib , Lesca Gaetan , Trujillo Carlos , Alwasiyah Mohammad Khalid , Munnich Arnold 
Published: May 2015

Key Clinical MessageX‐linked intellectual deficiency (XLID) is a large group of genetic disorders. MED12 gene causes syndromic and nonsyndromic forms of XLID. Only seven pathological mutations have been identified in this gene. Here, we report a novel mutation segregating with XLID phenotype. This mutation could be in favor of genotype–phenotype correlations.
Quantum Chemical Study of Water Adsorption on the Surfaces of SrTiO3 Nanotubes
Authors: Bandura Andrei V. , Kuruch Dmitry D. , Evarestov Robert A. 
Published: May 2015

AbstractWe have studied the adsorption of water molecules on the inner and outer surfaces of nanotubes generated by rolling (001) layers of SrTiO3 cubic crystals. The stability and the atomic and electronic structures of the adsorbed layers are determined by using hybrid density functional theory. The absorption energy and the preferred adsorbate structure are essentially governed by the nature of the surface of the nanotube. Dissociative adsorption prevails on the outer nanotube surfaces. The stability of the adsorbed layers on the inner surfaces is related to the possibility of the formation of hydrogen bonds between water molecules and surface oxygen atoms, and depends on the surface curvature. The presence of water molecules on the inner surface of the nanotubes leads to an increase of the electronic band gap. Externally TiO2‐terminated nanotubes could be used for the photocatalytic decomposition of water by ultraviolet radiation.
An environmental problem hidden in plain sight? Small Human-made ponds, emergent insects, and mercury contamination of biota in the Great Plains
Authors: Chumchal Matthew M. , Drenner Ray W. 
Published: May 2015

Abstract

Mercury (Hg) contamination of small human-made ponds and surrounding terrestrial communities may be 1 of the largest unstudied Hg-pollution problems in the United States. Humans have built millions of small ponds in the Great Plains of the United States, and these ponds have become contaminated with atmospherically deposited mercury. In aquatic ecosystems, less toxic forms of Hg deposited from the atmosphere are converted to highly toxic methylmercury (MeHg). Methylmercury is incorporated into the aquatic food web and then can be transferred to terrestrial food webs via emergent aquatic insects. The authors present a conceptual model that describes the movement of MeHg produced in aquatic ecosystems to terrestrial consumers via insects emerging from small human-made ponds. The authors hypothesize that pond permanence and the level of Hg contamination of the food web control this emergent insect-mediated flux of MeHg. The highest insect-mediated flux of MeHg is predicted to be from fishless semipermanent ponds with food webs that are highly contaminated with MeHg. Further development and testing of the conceptual model presented in the present column, particularly in the context of a changing climate, will require research at the regional, watershed, and pond scales. Environ Toxicol Chem 2015;34:1197–1205. © 2015 SETAC
A field test of the effect of spiked ivermectin concentrations on the biodiversity of coprophagous dung insects in Switzerland
Authors: Jochmann Ralf , Lipkow Erhard , Blanckenhorn Wolf U. 
Published: May 2015

Abstract

Veterinary medical product residues can cause severe damage in the dung ecosystem. Depending on the manner of application and the time after treatment, the excreted concentration of a given pharmaceutical varies. The popular anthelmintic drug ivermectin can be applied to livestock in several different ways and is fecally excreted over a period of days to months after application. In a field experiment replicated in summer and autumn, the authors mixed 6 ivermectin concentrations plus a null control into fresh cow dung to assess the reaction of the dung insect community. Taxon richness of the insect dung fauna emerging from the dung, but not Hill diversity (1D) or the total number of individuals (abundance), decreased as ivermectin concentration increased. Corresponding declines in the number of emerging insects were found for most larger brachyceran flies and hymenopteran parasitoids, but not for most smaller nematoceran flies or beetles (except Hydrophilidae). Parallel pitfall traps recovered all major dung organism groups that emerged from the experimental dung, although at times in vastly different numbers. Ivermectin generally did not change the attractiveness of dung: differences in emergence therefore reflect differences in survival of coprophagous offspring of colonizing insects. Because sample size was limited to 6 replicates, the authors generally recommend more than 10 (seasonal) replicates and also testing higher concentrations than used in the present study as positive controls in future studies. Results accord with parallel experiments in which the substance was applied and passed through the cow's digestive system. In principle, therefore, the authors' experimental design is suitable for such higher-tier field tests of the response of the entire dung community to pharmaceutical residues, at least for ivermectin. Environ Toxicol Chem 2016;35:1947–1952. © 2015 SETAC
Cubic spline extrapolation for uplink channel quality prediction in LTE‐A with carrier aggregation
Authors: Lema Maria A. , García‐Lozano Mario , Ruiz Silvia 
Published: May 2015

AbstractIn the context of wireless communications, an accurate channel state information is the key to a correct radio resource management. In the uplink of long‐term evolution advanced, obtaining up‐to‐date channel state information relies on user equipments that send sounding signals throughout the available bandwidth. This procedure is quite challenging when considering the ever increasing bandwidth demands and the introduction of carrier aggregation. Information from many radio resource blocks becomes outdated and unreliable for scheduling operations. Besides, interference values are much more variant than in the downlink due to changes in the position of interferers and their transmission power. This work proposes the introduction of a cubic spline extrapolation method to obtain a prediction horizon that allows extending the reliability of the channel quality evaluation along time. Although very high interference levels and variations thereof imply no gains, the mechanism succeeds with moderate interference, situation that can be provided by interference cancellation strategies. Copyright © 2015 John Wiley & Sons, Ltd.
New Limonoids from the Seeds of Xylocarpus granatum
Authors: Wu Yi‐Bing , Liu Dan , Liu Pei‐Yu , Yang Xue‐Mei , Liao Man , Lu Nan‐Nan , Sauriol Françoise , Gu Yu‐Cheng , Shi Qing‐Wen , Kiyota Hiromasa , Dong Mei 
Published: May 2015

AbstractThree novel limonoids, 2,3‐dideacetylxyloccensin S (1), 30‐deacetylxyloccensin W (2), and 7‐hydroxy‐21β‐methoxy‐3‐oxo‐24,25,26,27‐tetranortirucalla‐1,14‐diene‐23(21)‐lactone (3), were isolated from the seeds of the Chinese mangrove, Xylocarpus granatum. The structures were elucidated on the basis of 1D‐ and 2D‐NMR (including 1H‐ and 13C‐NMR, DEPT, 1H,1H‐COSY, HSQC, HMBC, and NOESY) data and confirmed by HR‐MS.
Three New Xanthones from the Fungus Penicillium sp. NH‐7‐1
Authors: Zhuang Yi‐Bin , Yin Hua , Zhang Xue‐Wen , Zhou Wei , Liu Tao 
Published: May 2015

AbstractThree new xanthones, drimiopsins G–I (1–3, resp.), and two known congeners, griseoxanthone C (4) and norlichexanthone (5), were isolated from a fungal isolate, Penicillium sp. NH‐7‐1. The structures of all compounds were determined on the basis of extensive spectroscopic analyses, as well as by comparison with literature reports, and the structure of compound 1 was further confirmed by single‐crystal X‐ray diffraction.
Facile Synthesis of the Pentasaccharide Repeating Unit of the Exopolysaccharide from Cryptococcus neoformans Serotype D
Authors: Zhuo Xiao‐Bin , Zou Yan , Huang Sheng‐Jun , Liao Jun , Hu Hong‐Gang , Zhao Qing‐Jie , Wu Qiu‐Ye 
Published: May 2015

Abstractβ‐D‐GlcpA‐(1→2)‐[β‐D‐Xylp‐(1→2)‐α‐D‐Manp‐(1→3)]‐α‐D‐Manp‐(1→3)‐α‐D‐Manp, the repeating unit of the exopolysaccharide from Cryptococcus neoformans serotype D, was synthesized as its 4‐methoxyphenyl glycoside. The approach presented here also provides a route to the synthesis of more complex repeating units of glucuconoxylomannan (GXM) of C. neoformans serotypes A–C.
New Calix[4]arene‐Cored Peripherally Functionalized Dendrimers: Synthesis and Conformational Characteristics
Authors: Paraskevopoulos Georgios , Alivertis Dimitrios , Theodorou Vassiliki , Skobridis Konstantinos 
Published: May 2015

AbstractNew calixarene‐based dendrimers, containing calix[4]arene as the core and different generations of Fréchet‐type poly(benzyl ether) dendrons as building blocks, which possess either Br‐atoms or COOtBu groups at their surface were synthesized and presented herein for the first time. The new calix[4]arene‐cored dendritic macromolecules were fully characterized and found to prefer strictly the cone conformation.
A New Indole Alkaloid from Cleome droserifolia
Authors: Hussain Javid , Khan Hidayatullah , Ali Liaqat , Latif Khan Abdul , Ur Rehman Najeeb , Jahangir Sajid , Al‐Harrasi Ahmed 
Published: May 2015

AbstractThe phytochemical investigations on Cleome droserifolia resulted in the isolation and characterization of a new indole alkaloid, 5‐hydroxy‐2‐methoxy‐1‐methyl‐1H‐indole‐3‐carbaldehyde (1). The structure elucidation was carried out on the basis of 1D‐ and 2D‐NMR techniques. In addition to 1, two known aromatic derivatives, veratrol (2) and 2‐methoxy‐4‐methylacetophenone (3), were also obtained. All these compounds were purified by repeated column chromatography of the CH2Cl2 fraction obtained from MeOH extract of Cleome droserifolia. The structure of the new compound 1 was finally confirmed by the combined 1D (1H‐ and 13C‐) and 2D (HC correlations; HMBC and HSQC) NMR and IR spectroscopy, mass spectrometry (MS), and UV absorption spectroscopy techniques. The comparison of the physical and spectroscopic data with those in the literature provided evidence for the structure confirmation of known compounds. All the purified compounds were subjected to urease and α‐glucosidase enzymes inhibition. The results showed that compound 1 was more potent with an IC50 value 11.97±2.067 μg/ml towards urease inhibition, while the activity of α‐glucosidase enzyme was marginal.
Explaining pharmacophobia and pharmacophilia in psychiatric patients: relationship with treatment adherence
Authors: De las Cuevas Carlos , Peñate Wenceslao 
Published: May 2015

ObjectiveThe objective of this study is to assess psychiatric patient attitudes towards psychotropic medication and their association with adherence, as well as to identify the factors that influence these attitudes.MethodsNine hundred and forty nine consecutive psychiatric outpatients categorized as “pharmacophobic” or “pharmacophilic” according to the Drug Attitude Inventory were compared with regard to adherence to prescribed treatment, socio‐demographic variables, clinical characteristics, health locus of control, self‐efficacy, and psychological reactance.ResultsSeventy three per cent of psychiatric outpatients showed a “pharmacophilic” attitude and 15.5% “pharmacophobic” one. Patients' attitudes toward prescribed drug treatment were clearly related to self‐reported treatment adherence. The pharmacophobic group registered significant worse adherence than the pharmacophilic patients. Psychiatric patients’ attitudes to drugs were mediated by external health locus of control, cognitive psychological reactance, and self‐efficacy.ConclusionThe encouragement of psychiatric patients’ treatment adherence needs to include lack of coercion during prescription, a positive relationship with the prescriber, verbal persuasion, information containing vicarious experience, and the involvement of the patient in treatment decisions. Copyright © 2015 John Wiley & Sons, Ltd.
Effects of iron oxide nanoparticles on biological responses and MR imaging properties in human mammary healthy and breast cancer epithelial cells
Authors: Zhang Qin , Rajan Sunder S. , Tyner Katherine M. , Casey Brendan J. , Dugard Christopher K. , Jones Yvonne , Paredes Angel M. , Clingman Chekesha S. , Howard Paul C. , Goering Peter L. 
Published: May 2015

AbstractSuperparamagnetic iron oxide nanoparticles (SPIONs, diameters >50 nm) have received great attention due to their promising use as magnetic resonance imaging (MRI) contrast agents. In this study, we evaluated the cellular uptake and biological responses in vitro of ultrasmall SPIONs (USPIONs, diameters < 50 nm). We compared the cellular responses between breast epithelia isolated from healthy and breast cancer donors after exposure to carboxy‐terminated USPIONs (10 and 30 nm PEG‐coated, 10 and 30 nm non‐PEG‐coated). The particles were characterized using transmission electron microscopy (TEM), dynamic light scattering (DLS) and gel electrophoresis. Cellular interactions with USPIONs were assessed by confocal microscopy and TEM. Cellular uptake of USPIONs was quantified using ICP‐MS. Cell viability was measured by MTT and neutral red uptake assays. T2* weighted MRI scans were performed using a 7T scanner. Results demonstrated that cell association/internalization of USPIONs was size‐ and surface coating‐dependent (PEG vs. non‐PEG), and higher cellular uptake of 10 and 30 nm non‐coated particles was observed in both cell types compared with PEG‐coated particles. Cell uptake for 10 and 30 nm non‐coated particles was higher in cancer cells from two of three tested donors compared to healthy cells from three donors. There was no significant cytotoxicity observed for all tested particles. Significantly enhanced MRI contrast was observed following exposure to 10 and 30 nm non‐coated particles compared to PEG‐coated particles in both cell types. In comparison, cancer cells showed more enhanced MRI signals when compared to normal cells. The data indicate that cell responses following exposure to USPIONs are dependent on particle properties. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1032–1042, 2016.
Apparent diffusion coefficient is highly reproducible on preclinical imaging systems: Evidence from a seven‐center multivendor study
Authors: Doblas Sabrina , Almeida Gilberto S. , Blé François‐Xavier , Garteiser Philippe , Hoff Benjamin A. , McIntyre Dominick J.O. , Wachsmuth Lydia , Chenevert Thomas L. , Faber Cornelius , Griffiths John R. , Jacobs Andreas H. , Morris David M. , O'Connor James P.B. , Robinson Simon P. , Van Beers Bernard E. , Waterton John C. 
Published: May 2015

PurposeTo evaluate between‐site agreement of apparent diffusion coefficient (ADC) measurements in preclinical magnetic resonance imaging (MRI) systems.Materials and MethodsA miniaturized thermally stable ice‐water phantom was devised. ADC (mean and interquartile range) was measured over several days, on 4.7T, 7T, and 9.4T Bruker, Agilent, and Magnex small‐animal MRI systems using a common protocol across seven sites. Day‐to‐day repeatability was expressed as percent variation of mean ADC between acquisitions. Cross‐site reproducibility was expressed as 1.96 × standard deviation of percent deviation of ADC values.ResultsADC measurements were equivalent across all seven sites with a cross‐site ADC reproducibility of 6.3%. Mean day‐to‐day repeatability of ADC measurements was 2.3%, and no site was identified as presenting different measurements than others (analysis of variance [ANOVA] P = 0.02, post‐hoc test n.s.). Between‐slice ADC variability was negligible and similar between sites (P = 0.15). Mean within‐region‐of‐interest ADC variability was 5.5%, with one site presenting a significantly greater variation than the others (P = 0.0013).ConclusionAbsolute ADC values in preclinical studies are comparable between sites and equipment, provided standardized protocols are employed. J. MAGN. RESON. IMAGING 2015;42:1759–1764.