bluuebunny/crossref_metadata_2025_split huggingface dataset:
Male breast cancer according to tumor subtype and race
Authors: Chavez‐MacGregor Mariana , Clarke Christina A. , Lichtensztajn Daphne , Hortobagyi Gabriel N. , Giordano Sharon H. 
Published: January 2013

AbstractBACKGROUND:Breast cancer occurs rarely in men. To the authors' knowledge, no population‐based estimates of the incidence of human epidermal growth factor receptor 2 (HER2)‐positive breast cancer or of the distribution of breast cancer subtypes among male breast cancer patients have been published to date. Therefore, the objective of the current study was to explore breast tumor subtype distribution by race/ethnicity among men in the large, ethnically diverse population of California.METHODS:This study included men who were diagnosed with invasive breast cancer between 2005 and 2009 with known estrogen receptor (ER) and progesterone receptor (PR) (together, hormone receptor [HR]) status and HER2 status reported to the California Cancer Registry. Among the men with HR‐positive tumors, survival probabilities between groups were compared using log‐rank tests.RESULTS:Six hundred six patients were included. The median age at diagnosis was 68 years. Four hundred ninety‐four men (81.5%) had HR‐positive tumors (defined as ER‐positive and/or PR‐positive and HER2‐negative). Ninety men (14.9%) had HER2‐positive tumors, and 22 (3.6%) had triple receptor‐negative (TN) tumors. Among the patients with HR‐positive tumors, non‐Hispanic black men and Hispanic men were more likely to have PR‐negative tumors than non‐Hispanic white men. No statistically significant differences in survival were observed according to tumor subtype (P = .08). Differences in survival according to race/ethnicity were observed among all patients (P = .087) and among those with HR‐positive tumors (P = .0170), and non‐Hispanic black men had poorer outcomes.CONCLUSIONS:In this large, representative cohort of men with breast cancer, the distribution of tumor subtypes was different from that reported for women and varied by patient race/ethnicity. Non‐Hispanic black men were more likely to have TN tumors and ER‐positive/PR‐negative tumors than white men. Cancer 2013. © 2013 American Cancer Society.
Soluble FAS ligand as a biomarker of disease recurrence in differentiated thyroid cancer
Authors: Owonikoko Taofeek K. , Hossain Mohammad S. , Bhimani Chandar , Chen Zhengjia , Kim Sungjin , Ramalingam Suresh S. , Sun Shi‐Yong , Shin Dong M. , Waller Edmund K. , Khuri Fadlo R. 
Published: January 2013

AbstractBACKGROUND:Reliable predictive biomarkers are required to address the challenge of disease recurrence after thyroid cancer surgery. For this study, the authors assessed the association of cellular‐based and serum‐based immunologic mediators with thyroid cancer recurrence.METHODS:Leukocyte subset counts and immune regulatory cytokine levels were determined in peripheral blood samples using multiparameter flow cytometry and 51‐panel, multiplex enzyme‐linked immunosorbent assays, respectively. The functional activity of circulating B‐lymphocytes, T‐lymphocytes, and natural killer lymphocytes was assessed ex vivo. Differences in mean biomarker levels between defined patient groups and correlations between biomarkers and cancer recurrence were assessed using t tests or Wilcoxon tests and by univariate and multivariate analyses with Cox models. Optimal cutoff values of significantly correlated biomarkers that best predicted disease recurrence after surgery were established by receiver operating characteristics and were validated by using an optimal cutpoint determination algorithm.RESULTS:In total, 35 patients were enrolled (median age, 49.4 year), including 24 women and 15 patients with recurrent disease; and there were 21 individuals in the control group. Patients without recurrence had higher levels of soluble FAS (tumor necrosis receptor superfamily, member 6) ligand (sFASL), transforming growth factor‐β, regulatory T cells, and programmed death 1/ programmed death ligand 1‐expressing leukocytes. sFASL (hazard ratio, 0.60; 95% confidence interval, 0.38‐0.95; P = .031) and interferon‐α (hazard ratio, 1.55; 95% confidence interval, 1.03‐2.34; P = .038) were associated significantly with disease recurrence. There was a significant difference in progression‐free survival between patient groups stratified by an sFASL optimal cutpoint of 15 pg/mL (log‐rank P = .0009).CONCLUSIONS:sFASL and IFN‐α levels were correlated significantly with thyroid cancer recurrence and may be useful for risk‐adapted surveillance strategies in patients with thyroid cancer. Cancer 2013. © 2013 American Cancer Society.
Characterization of the hypothalamus of Xenopus laevis during development. I. The alar regions
Authors: Domínguez Laura , Morona Ruth , González Agustín , Moreno Nerea 
Published: January 2013

AbstractThe patterns of expression of a set of conserved developmental regulatory transcription factors and neuronal markers were analyzed in the alar hypothalamus of Xenopus laevis throughout development. Combined immunohistochemical and in situ hybridization techniques were used for the identification of subdivisions and their boundaries. The alar hypothalamus was located rostral to the diencephalon in the secondary prosencephalon and represents the rostral continuation of the alar territories of the diencephalon and brainstem, according to the prosomeric model. It is composed of the supraoptoparaventricular (dorsal) and the suprachiasmatic (ventral) regions, and limits dorsally with the preoptic region, caudally with the prethalamic eminence and the prethalamus, and ventrally with the basal hypothalamus. The supraoptoparaventricular area is defined by the orthopedia (Otp) expression and is subdivided into rostral and caudal portions, on the basis of the Nkx2.2 expression only in the rostral portion. This region is the source of many neuroendocrine cells, primarily located in the rostral subdivision. The suprachiasmatic region is characterized by Dll4/Isl1 expression, and was also subdivided into rostral and caudal portions, based on the expression of Nkx2.1/Nkx2.2 and Lhx1/7 exclusively in the rostral portion. Both alar regions are mainly connected with subpallial areas strongly implicated in the limbic system and show robust intrahypothalamic connections. Caudally, both regions project to brainstem centers and spinal cord. All these data support that in terms of topology, molecular specification, and connectivity the subdivisions of the anuran alar hypothalamus possess many features shared with their counterparts in amniotes, likely controlling similar reflexes, responses, and behaviors.
Sequential staining improves detection of CCR2 and CX3CR1 on monocytes when simultaneously evaluating CCR5 by multicolor flow cytometry
Authors: Jalbert Emilie , Shikuma Cecilia M. , Ndhlovu Lishomwa C. , Barbour Jason D. 
Published: January 2013

AbstractChemokines and their receptors play an essential role within the immune system by dictating cellular migration. In vivo, receptor–ligand interactions rarely occur in isolation as cellular recruitment and migration are complex and highly coordinated processes often involving networks of multiple chemokines and multiple receptors. Simultaneous detection of multiple chemokine receptors on the single cell level is necessary to allow immunophenotyping studies that will help understand the intricacies of these networks. Chemokine receptors undergo a basal level of ongoing internalization, intracellular trafficking, and recycling back to the cell surface, even in the absence of the ligand. In the presence of ligand, receptor–ligand interactions enhance receptor internalization, reducing the cell surface receptor concentration, making precise determination of intrinsic levels challenging. Using multicolor flow cytometry, we sought to evaluate and optimize the simultaneous detection of cell surface expression levels of CCR2, CX3CR1, and CCR5 in primary human monocytes using a single antibody panel. We observed that staining for CCR2 alone or for CX3CR1 alone showed greater expression levels than when the cells were stained with the full panel of antibodies. Fluorescent‐minus‐one (FMO) controls revealed that ligation of the CCR5 monoclonal antibody to the receptor interfered with detection of CX3CR1 and CCR2. Sequential addition of antibodies during the staining procedure was sufficient to restore the detection levels, suggesting close proximity and possible functional interactions between CCR2/CCR5 and CX3CR1/CCR5 in monocytes. This study highlights the importance of optimizing staining procedures and using proper controls when simultaneously evaluating expression levels of multiple chemokine receptors by flow cytometry. Concurrent assessment of multiple receptors will provide insight and greater understanding of the complex interactions involved in cellular migration. © 2013 International Society for Advancement of Cytometry
Hepatic endometriosis diagnosed by liquid‐based cytology: A Case Report
Authors: Hertel J. D. , Guimaraes M. , Yang J. 
Published: January 2013

A 44‐year‐old woman presented to Emergency Department with sudden onset of severe upper abdominal pain. T2‐weighted MRI image showed a large cystic mass with a thickened wall measuring 9.5 × 9.1 × 11.2 cm in the right hepatic lobe. It was radiologically interpreted as a cystic mass with differential diagnosis including echinococcal cyst, biliary cystadenoma, and malignant neoplasm. The cystic mass was intraoperatively aspirated and a liquid‐based cytology preparation (ThinPrep) and a cell block were made. The ThinPrep slides showed three dimensional clusters of epithelioid cells with scant delicate cytoplasm and tissue fragments composed of small stromal cells with round to oval shaped nuclei and a small amount of dense cytoplasm lined by the cuboidal epithelial cells. Occasional ciliated cells and abundant hemosiderin laden macrophages were also present. The cell block showed many tissue fragments containing glands and stroma with associated hemorrhage and hemosiderin laden macrophages, typical of endometrial tissue.Although it is uncommon, hepatic endometriosis should be considered in the differential diagnosis of cystic liver mass in women, especially those with a history of endometriosis or obstetric/gynecologic surgery. Diagn. Cytopathol. 2014;42:441–444. © 2013 Wiley Periodicals, Inc.
Medullary breast carcinoma in an 18‐year‐old female: Report on one case diagnosed on fine‐needle cytology sample
Authors: Galzerano Antonio , Rocco Nicola , Accurso Antonello , Ciancia Giuseppe , Campanile Anna Cipolletta , Caccavello Ferdinando , Fulciniti Franco 
Published: January 2013

Medullary breast carcinoma (MBC) is a rare epithelial malignancy of the breast accounting for about 1–7% of all breast carcinomas. It is characterized by well‐defined borders, a syncytial/solid pattern of growth of high grade atypical cells showing no glandular differentiation and a massive diffuse lympho‐plasmacytic peritumoral infiltrate. Despite the high‐grade atypias characterizing this neoplasm, MBC has been reported to have a better prognosis when compared with the common infiltrating duct carcinoma. MBCs typically lack estrogen and progesterone receptor (ER and PgR) expression and have a low incidence of ERBB2 overexpression. Genetically, they are often associated with BRCA‐1 oncogene mutations and TP53 alterations.While MBC generally occurs in middle‐aged women, ranging from 45 to 52 years of age, we report the case of a 18‐year‐old female patient which was diagnosed by means of fine‐needle cytology sample. Diagn. Cytopathol. 2014;42:445–448. © 2013 Wiley Periodicals, Inc.
Developmental trajectories of respiratory sinus arrhythmia: Associations with social responsiveness
Authors: Patriquin Michelle A. , Lorenzi Jill , Scarpa Angela , Bell Martha Ann 
Published: January 2013

AbstractThe present longitudinal study examined relations between respiratory sinus arrhythmia (RSA) development and social responsiveness characteristics associated with autism spectrum disorders. Group‐based developmental trajectory modeling was used to characterize RSA development patterns in 106 typically developing children across 5, 10, 24, 36, and 48 months of age. A two‐group model fit of RSA development was found: a “typically” and “atypically” developing group. The typical group gradually increased in RSA across 5–48 months of age. The atypical group, however, increased in RSA from 5 to 24 months and demonstrated a plateau or “delay” in RSA development from 24 to 48 months. The atypical RSA development group also demonstrated more difficulties in parent‐reported social responsiveness at 48 months. The results support current literature that identifies RSA as a marker of social functioning level. © 2013 Wiley Periodicals, Inc. Dev Psychobiol 56: 317–326, 2014.
Long‐period observation of neutral current at an extra‐high‐voltage transformer in Chubu region
Authors: Izutsu Jun , Nagata Satoru , Wada Tadayuki , Shimizu Masahito , Ohta Kenji 
Published: January 2013

AbstractWe have observed and recorded the neutral current of a 500‐kV transformer at a substation in the Chubu region since 2008 in order to detect electromagnetic anomalies associated with earthquakes. In this study, we could not ﬁnd any particular anomalous change of the neutral current before and after earthquakes (e.g., for the Suruga Bay Earthquake of August 11, 2009). However, from the results of a quantitative analysis between geomagnetic ﬁeld data and neutral current data, we would be able to investigate the underground electric conductivities near the substations. It would be possible to monitor changes of subsurface electromagnetic structure before and after earthquakes. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 183(1): 32–38, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.22297
Design of a servo control system using a notch filter for a flexible arm and its applications
Authors: Mori Takahiko 
Published: January 2013

AbstractThis paper proposes a new design method for a frequency‐dependent servo system for the positioning of flexible structures. In order to reduce sensor costs and to suppress spillover phenomena caused by ignored elastic modes of the plant, we introduce a prefilter into an input which possesses the characteristics of a notch filter. The proposed method allows for the design of the specified characteristics of the prefilter and for precise assignment of poles to desirable locations in a closed‐loop system. As a result, the tradeoﬀ problem between suppression and transients can be resolved. The results of simulations and experiments are given in order to verify the usefulness of this method. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 183(1): 39–50, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.22343
Location feasibility of degradation in cable through Fourier transform analysis of broadband impedance spectra
Authors: Ohki Yoshimichi , Hirai Naoshi 
Published: January 2013

AbstractSince electric cables play important roles such as power supply and information transmission, their degradation may cause serious problems. We have been trying to monitor the degradation of cable insulation by measuring the magnitude and phase angle of the impedance as a function of frequency in a very wide frequency range. The cables tested were insulated with ﬂame‐retardant ethylene propylene rubber or special heat‐resistant polyvinyl chloride. They were partially damaged by peeling off their insulation layers, or partially aged by heat and γ‐rays. The impedance and phase angle were measured from a terminal of the cable. The difference in impedance between the damaged and sound cables is made clear by fast Fourier transform analyses, from which the damaged portion can be located. It can be clearly shown that this method has the potential ability to detect the degradation of cable insulation induced by physical damage, γ‐ray irradiation, and thermal aging. © 2013 Wiley Periodicals, Inc. Electr Eng Jpn, 183(1): 1–8, 2013; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.22384
Determination of Tetracycline at a UV‐Irradiated DNA Film Modified Glassy Carbon Electrode
Authors: Gholivand Mohammad Bagher , Khani Hamzeh 
Published: January 2013

AbstractIn this study, interaction of tetracycline (TC) and DNA in the BrittonRobinson buffer solution (BR) was studied by cyclic voltammetry. The experimental results reveal that TC can bind strongly to DNA and the association constant and binding number between TC and DNA was obtained. Then DNA was immobilized on a glassy carbon electrode by UV‐irradiation. Through this process, water‐soluble DNA was converted into insoluble materials, and a stable DNA film was formed on the electrode. The electrochemical oxidation behavior of TC was studied at UV‐irradiated DNA film modified glassy carbon electrode (UV‐DNA‐GCE). The response of modified electrode was optimized with respect to pH, accumulation time, ionic strength, drug concentration and other variables. TC at the surface of modified electrode showed a linear dynamic range of 0.30–90.00 µM and a detection limit of 0.27 µM. To demonstrate the applicability of the modified electrode, it was used for the analysis of real samples such as pharmaceutical formulations and milk.
Preparation and Characterization of Hybrid Oxyethylene/Siloxane Electrolyte Systems
Authors: Rodrigues Luísa C. , Silva Maria M. , Smith Michael J. , Gonçalves A. , Fortunato E. 
Published: January 2013

AbstractOne of the most significant consequences of technological evolution in the workplace has been a dramatic increase in the need for portable energy storage. This evolution has occurred, not only with respect to the number of devices, but also in their average energy storage capacity. An obvious commercial consequence has been an increased pressure to develop improved active materials for power sources and more efficient methods for battery production. In recent decades the growth of the commercial market for high performance batteries has been based on the development of both solid‐state and gel electrolytes. The incorporation of these electrolytes as components of various devices (advanced batteries, smart windows, displays and super‐capacitors) offers significant advantages relative to traditional electrolytes, including enhanced reliability and improved safety. The xerogel matrices prepared in this study are represented as U(900) and U(600) and contain 15.5 or 8.5 oxyethylene structural units (CH2CH2O) respectively. The oxyethylene chains are bonded at each end to a siliceous intersection through urea bridging links. These sol‐gel derived oxyethylene /siloxane xerogels (designated as di‐ureasils) were doped with controlled amounts of LiAsF6 or LiSbF6 to prepare a range of electrolyte compositions. The compositions prepared, with 200>n≥2.5 (where the salt content is expressed as n, the molar ratio of oxyethylene moieties to Li+ ions), were characterized by electrochemical and thermal techniques. Preliminary tests performed with a prototype electrochromic device (ECD) incorporating the most promising electrolyte composition, d‐U(900)8LiAsF6 as electrolyte and WO3 as cathodic electrochromic layer, are extremely encouraging.
Electrodeposition of PdAu Alloy Nanoparticles on Ionic Liquid Functionalized Graphene Film for the Voltammetric Determination of Oxalic Acid
Authors: Shang Lei , Zhao Faqiong , Zeng Baizhao 
Published: January 2013

AbstractAn ionic liquid functionalized graphene film was prepared and PdAu nanoparticles (NPs) were electrodeposited on it. The PdAu NPs were characterized by various methods and they showed the features of alloys. In 0.2 M H2SO4 solution, oxalic acid (OA) exhibited a sensitive anodic peak at the resulting electrode at about 1.1 V (vs. SCE), and the peak current was linear to OA concentration in the range of 5–100 µM with a sensitivity of 45.5 µA/mM. The detection limit was 2.7 µM (S/N=3). The electrode was successfully applied to the determination of OA in real sample.
The role of silver and vanadium release in the toxicity of silver vanadate nanowires toward Daphnia similis
Authors: Artal Mariana Coletty , Holtz Raphael Dias , Kummrow Fábio , Alves Oswaldo Luiz , Umbuzeiro Gisela de Aragão 
Published: January 2013

Abstract
Nanomaterials are used in a wide spectrum of applications, including nanowires that are objects with at least one of its dimensions in the range of 1 to 100 nm. Recently, a new type of silver vanadate nanowire decorated with silver nanoparticles (SVSN-LQES1) with promising antimicrobial activity against different pathogenic bacteria was described. The objective of the present study was to evaluate the role of silver and vanadium release in the acute toxicity of this material using Daphnia similis. To verify the effect of the presence of the nanowires in the test solution, tests were performed before and after filtration. Total silver release to the testing media was determined using the method of inductively coupled plasma atomic emission spectroscopy (ICP-AES). Silver vanadate nanowires decorated with silver nanoparticles (SVSN-LQES1) are acutely toxic to D. similis. The release of silver from the nanomaterial trapped in the gut along with the silver released to the test media seems to be responsible for the observed toxicity. Although toxic to Daphnia, vanadium does not contribute to the toxicity of SVSN-LQES1. The observed increase in lipid droplets appears to be related to the exposure of the organisms to the nanomaterials, but the significance of this response needs further investigation. Environ. Toxicol. Chem. 2013;32:908–912. © 2013 SETAC
Accumulation of dietary methylmercury and effects on growth and survival in two estuarine forage fish: Cyprinodon variegatus and Menidia beryllina
Authors: Stefansson Emily S. , Heyes Andrew , Rowe Christopher L. 
Published: January 2013

Abstract
Dietary methylmercury (MeHg) uptake by fish in relation to life stage, species, and level of exposure is poorly understood in lower trophic levels, particularly in estuarine species. The authors compared accumulation of dietary MeHg as well as sensitivity (survival and growth) to dietary MeHg exposure in two species of estuarine forage fish, Cyprinodon variegatus and Menidia beryllina. Fish were fed one of five dietary MeHg concentrations (ranging from 0.04 to 14 µg/g dry wt) over a period of 70 d. Growth rate and the level of dietary exposure influenced MeHg tissue concentrations in both species. Mercury in the diet exhibited a strong linear relationship with fish Hg tissue concentrations. Additionally, the authors found that M. beryllina was more sensitive to dietary MeHg exposure than C. variegatus. Both species showed some decreases in growth related to MeHg exposure, although these patterns were not consistent among treatments. Overall, C. variegatus and M. beryllina were found to have a high tolerance for dietary MeHg exposure. If fish occupying low trophic levels are capable of surviving with high Hg body burdens, this tolerance has important implications for Hg exposure of organisms occupying higher trophic levels. Environ. Toxicol. Chem. 2013;32:848–856. © 2013 SETAC