bluuebunny/crossref_metadata_2025_split huggingface dataset:
Seed production determines the entrance to dormancy of the inflorescence meristem of Pisum sativum and the end of the flowering period
Authors: Burillo Eduardo , Ortega Raul , Vander Schoor Jacqueline K. , Martínez‐Fernández Irene , Weller James L. , Bombarely Aureliano , Balanzà Vicente , Ferrándiz Cristina 
Published: July 2024

AbstractFlowering plants adjust their reproductive period to maximize the success of the offspring. Monocarpic plants, those with a single reproductive cycle that precedes plant senescence and death, tightly regulate both flowering initiation and flowering cessation. The end of the flowering period involves the arrest of the inflorescence meristem activity, known as proliferative arrest, in what has been interpreted as an evolutionary adaptation to maximize the allocation of resources to seed production and the viability of the progeny. Factors influencing proliferative arrest were described for several monocarpic plant species many decades ago, but only in the last few years studies performed in Arabidopsis have allowed to approach proliferative arrest regulation in a comprehensive manner by studying the physiology, hormone dynamics, and genetic factors involved in its regulation. However, these studies remain restricted to Arabidopsis and there is a need to expand our knowledge to other monocarpic species to propose general mechanisms controlling the process. In this work, we have characterized proliferative arrest in Pisum sativum, trying to parallel available studies in Arabidopsis to maximize this comparative framework. We have assessed quantitatively the role of fruits/seeds in the process, the influence of the positional effect of these fruits/seeds in the behavior of the inflorescence meristem, and the transcriptomic changes in the inflorescence associated with the arrested state of the meristem. Our results support a high conservation of the factors triggering arrest in pea and Arabidopsis, but also reveal differences reinforcing the need to perform similar studies in other species.
The effect of exogenous melatonin administration before superovulation on embryo yield in Assaf ewes
Authors: Yesilkaya Omer Faruk , Erdem Huseyin 
Published: July 2024

AbstractThe aim of this study was to determine the effect of exogenous melatonin administration on transferable embryos by increasing total antioxidant status before superovulation in Assaf ewes. Selected ewes were randomly divided into two equal groups: melatonin (n = 9) and control (n = 9). In the melatonin group, a melatonin implant (18 mg melatonin, Regulin®, Ceva, Turkey) was placed under the skin of the ear 7 days prior to insertion of the progesterone‐containing sponge. In the control group, a physiological saline solution was injected under the skin of the ear on the same day. The same superovulation protocol was used in both groups. In addition, blood samples for determination of Glutathione peroxidase, superoxide dismutase, total antioxidant status and total oxidant status concentrations were collected on five different days, including the day of melatonin implant placement (Day−7), vaginal sponge insertion (Day 0), vaginal sponge removal (Day 11), mating (Day 12–13) and uterine flushing (Day 19). Embryos were collected by laparotomy on the 7th day after mating. Uterine flushing taken into petri dishes were scanned under a stereomicroscope, and the quality and developmental stages of the embryos were recorded. In the study, total corpus luteum count and total cell count were found to be higher in the control group than in the melatonin group (p < .05). When the results were evaluated in terms of oxidative stress index, a negative correlation was found between the total number of corpus luteum, number of cells obtained, count of transferable embryos and number of Grade 1 embryos on Day 0. There was also a positive correlation oxidative stress index and the number of unfertilized oocytes on Day−7. As a result, exogenous melatonin administration prior to superovulation during the breeding season is thought to have a negative effect on embryo yield and quality. Therefore, the use of exogenous melatonin in MOET studies during the breeding season is recommended to be investigated in new studies.
An empirical comparison of stabilization funds and futures hedging for oil exporting developing countries
Authors: Lalloo Ricardo 
Published: July 2024

AbstractOil price volatility can significantly affect the growth and development of oil exporting developing countries. Given the widespread use of stabilization funds as a risk management tool by such countries and the potential of futures hedging to function in a similar vein, no study has effectively compared both mechanisms. This article hence uses the hedging effectiveness measure to examine the potential of each mechanism to smooth the volatile revenues of oil exporting developing countries. The results show that stabilization funds performed best with larger deposit rates and using benchmark prices with larger moving averages. However, the volatility reduction capabilities of optimal futures hedging were found to be far superior and should thus be adopted by oil dependent developing countries as their prime revenue smoothening mechanism.
The Effect of Duration on Performance and Perceived Fatigability During Acute High‐Intensity Interval Exercise in Young, Healthy Males and Females
Authors: Tripp Thomas R. , Caswell Allison M. , Aboodarda S. Jalal , MacInnis Martin J. 
Published: July 2024

ABSTRACTFew studies have explored the kinetics of performance and perceived fatigability during high‐intensity interval training, despite its popularity. We aimed to characterize the kinetics of fatigability and recovery during an 8 × 4‐min HIIT protocol, hypothesizing that most muscle function impairment would occur during the initial four intervals. Fifteen healthy males and females (mean ± standard deviation; age = 26 ± 5 years, V̇O2max = 46.8 ± 6.1 mL·kg−1·min−1) completed eight, 4‐min intervals at 105% of critical power with 3 min of rest. Maximal voluntary knee extension contractions (MVCs) coupled with electrical nerve stimulation were performed at baseline and after the first, fourth, and eighth intervals. MVC, potentiated twitch force (Pt), and Db10:100 ratio all declined throughout HIIT (p < 0.05). MVC sharply declined after interval 1 (−15 ± 9% relative to baseline; p < 0.05) and had only further declined after interval 8 (−26 ± 11%; p < 0.05), but not interval 4 (−19 ± 13%; p > 0.05). Pt and Db10:100 also sharply declined after interval 1 (Pt: −18 ± 13%, Db10:100: −14 ± 20%; p < 0.05) and further declined after interval 4 (Pt: −35 ± 19%, Db10:100: −30 ± 20%; p < 0.05) but not interval 8 (Pt: −41 ± 19%; Db10:100: −32 ± 18%; p > 0.05). Voluntary activation did not significantly change across the HIIT protocol (p > 0.05). Evoked force recovery was significantly blunted as more intervals were completed: after interval 1, Pt recovered by 7 ± 11% compared to −6 ± 7% recovery after interval 8 (p < 0.05). Ratings of perceived effort, fatigue, and leg pain rose throughout the session (p < 0.05 for each) and were greater (effort and fatigue) for females (p < 0.05). Otherwise, males and females exhibited similar performance fatigability kinetics, with contractile function declines blunted in response to additional intervals.
The Influence of Sports‐Related Concussion on Cognition and Landing Biomechanics in Collegiate Athletes
Authors: Avedesian Jason M. , Covassin Tracey , Baez Shelby , Nash Jennifer , Dufek Janet S. 
Published: July 2024

ABSTRACTInjury surveillance data indicate that collegiate athletes are at greater risk for lower extremity (LE) injuries following sports‐related concussion (SRC). While the association between SRC and LE injury appears to be clinically relevant up to 1‐year post‐SRC, little evidence has been provided to determine possible mechanistic rationales. Thus, we aimed to compare collegiate athletes with a history of SRC to matched controls on biomechanical and cognitive performance measures associated with LE injury risk. Athletes with a history of SRC (n = 20) and matched controls (n = 20) performed unanticipated bilateral land‐and‐cut tasks and cognitive assessments. Group‐based analyses (ANOVA) and predictive modeling (C5.0 decision tree algorithm) were used to compare group differences on biomechanical and cognitive measures. Collegiate athletes with a history of SRC demonstrated approximately six degrees less peak knee flexion on both dominant (p = 0.03,d = 0.71) and nondominant (p = 0.02,d = 0.78) limbs during the land‐and‐cut tasks compared to controls. Verbal Memory, knee flexion, and Go/No Go total score (C5.0 decision tree algorithm) were identified as the strongest indicators of previous SRC injury history. Reduced knee flexion during sport‐specific land‐and‐cut tasks may be a mechanism for increased LE injury risk in athletes with a history of SRC. There appears to be multiple biomechanical and cognitive predictors for identifying previous SRC in collegiate athletes, providing evidence to support a multifactorial SRC management strategy to reduce future injury risk.
“I really got to think about my background, their background, and how do we come together on something?”: One emergent mathematics teacher leader's reflexive journey withSocial Justice Mathematics
Authors: Kokka Kari 
Published: July 2024

AbstractThis 2‐year qualitative case study focuses on one emergent mathematics teacher leader, Mr. Miller, and his conceptualization of Social Justice Mathematics (SJM). SJM is a justice‐oriented pedagogical approach where students simultaneously learn dominant mathematics and explore social injustices to take action toward justice. Using Rodriguez's (Journal of Research in Science Teaching, 1998, 35, 589–622) sociotransformative constructivism framework, findings illuminate how dialogic conversation, authentic activity, and metacognition supported Mr. Miller's reflexivity about his positionality, which he described as “upper middle class, highly educated parents, white, male,” in relationship to his students' positionality. He taught in a public charter high school in an urban city in the Northeast United States, where approximately 60% of students identified as Black, 30% white, 10% mixed race, 1% Asian American, 1% Latine, and less than 1% Indigenous, with 60% of students qualifying for free or reduced lunch. The article discusses of the importance of reflexivity for teachers and teacher leaders of all backgrounds, and especially when educators of dominant backgrounds work with students of historically marginalized backgrounds. The article calls for further research with more experienced mathematics teacher leaders of various backgrounds and contexts to further investigate justice‐centered mathematics teacher leadership.
Reading Paasi and Living through Bounded Spaces
Authors: Lulle Aija 
Published: July 2024

AbstractThis brief article dedicates a few subjective considerations to the versatility and afterlives of Paasi's conceptual thinking, enriching the way we perceive spaces and borders in highly differentiated environments and times. I will confess my uneasiness with bounded spaces and explore the origins of that unease. I draw inspiration from feminist geography, which embraces subjectivity, dispelling the myth of objectivity. Although not explicitly referenced by Paasi himself, Paasi's article implicitly poses similar feminist questions: Who gains? Who is overlooked and who loses within bounded spaces and ‘borderless’ orders? For whom are spatial and territorial borders beneficial, and whom do they oppress? I will further examine how the ideas of spaces, borders and boundaries have evolved in my specific interpretation of Paasi's writing. The text will be interspersed with insights into lived experiences of regions, products, brands and people's consciousness, or, as in my case, awkward embodied experiences.
Epigenetic regulation by hypoxia, N‐acetylcysteine and hydrogen sulphide of the fetal vasculature in growth restricted offspring: A study in humans and chicken embryos
Authors: Krause Bernardo J. , Paz Adolfo A. , Garrud Tessa A. C. , Peñaloza Estefanía , Vega‐Tapia Fabian , Ford Sage G. , Niu Youguo , Giussani Dino A. 
Published: July 2024

AbstractFetal growth restriction (FGR) is a common outcome in human suboptimal gestation and is related to prenatal origins of cardiovascular dysfunction in offspring. Despite this, therapy of human translational potential has not been identified. Using human umbilical and placental vessels and the chicken embryo model, we combined cellular, molecular, and functional studies to determine whether N‐acetylcysteine (NAC) and hydrogen sulphide (H2S) protect cardiovascular function in growth‐restricted unborn offspring. In human umbilical and placental arteries from control or FGR pregnancy and in vessels from near‐term chicken embryos incubated under normoxic or hypoxic conditions, we determined the expression of the H2S gene CTH (i.e. cystathionine γ‐lyase) (via quantitative PCR), the production of H2S (enzymatic activity), the DNA methylation profile (pyrosequencing) and vasodilator reactivity (wire myography) in the presence and absence of NAC treatment. The data show that FGR and hypoxia increased CTH expression in the embryonic/fetal vasculature in both species. NAC treatment increased aortic CTH expression and H2S production and enhanced third‐order femoral artery dilator responses to the H2S donor sodium hydrosulphide in chicken embryos. NAC treatment also restored impaired endothelial relaxation in human third‐to‐fourth order chorionic arteries from FGR pregnancies and in third‐order femoral arteries from hypoxic chicken embryos. This NAC‐induced protection against endothelial dysfunction in hypoxic chicken embryos was mediated via nitric oxide independent mechanisms. Both developmental hypoxia and NAC promoted vascular changes in CTH DNA and NOS3 methylation patterns in chicken embryos. Combined, therefore, the data support that the effects of NAC and H2S offer a powerful mechanism of human translational potential against fetal cardiovascular dysfunction in complicated pregnancy.
imageKey points
Gestation complicated by chronic fetal hypoxia and fetal growth restriction (FGR) increases a prenatal origin of cardiovascular disease in offspring, increasing interest in antenatal therapy to prevent against a fetal origin of cardiovascular dysfunction.
We investigated the effects between N‐acetylcysteine (NAC) and hydrogen sulphide (H2S) in the vasculature in FGR human pregnancy and in chronically hypoxic chicken embryos.
Combining cellular, molecular, epigenetic and functional studies, we show that the vascular expression and synthesis of H2S is enhanced in hypoxic and FGR unborn offspring in both species and this acts to protect their vasculature.
Therefore, the NAC/H2S pathway offers a powerful therapeutic mechanism of human translational potential against fetal cardiovascular dysfunction in complicated pregnancy.
Development of a 6-Degrees-of-Freedom Hybrid Interface Intended for Teleoperated Robotic Cervical Spine Surgery
Authors: Koszulinski Alizée , Sandoval Juan , Arsicault Marc , Laribi Med Amine 
Published: July 2024

Abstract
This article deals with the development of a 6-degrees-of-freedom (DoF) hybrid interface for a teleoperated robotic platform intended to assist surgeons in cervical spine surgery. The targeted task is the drilling of cervical vertebrae for the attachment of spinal implants. Given the complex anatomy of the cervical region, with the proximity of the spinal cord and vertebral arteries, high accuracy in the drilling procedure is required to avoid complications for the patient. In this context, the proposed hybrid interface has been designed to meet the requirements of the drilling task, in terms of degrees of freedom, workspace, and force feedback, which have been identified through a literature review. It consists of an association of two parallel mechanisms and a centrally located serial mechanism. Direct and inverse kinematic modeling of each mechanism and one of the complete interfaces were carried out. A study of the dexterity distribution of the parallel mechanisms was carried out to select the suitable interface working mode that would keep the singularities away from the prescribed workspace. In addition, the force feedback was implemented in static mode, neglecting in the first time the weight of the system. The interface design parameters were then optimized to avoid singularities within the prescribed workspace, to minimize motor torques, and to reduce the size of the interface. These development stages led to the design of a motorized prototype of the hybrid interface.
Physiologic medium renders human iPSC-derived macrophages permissive for
M. tuberculosis
by rewiring organelle function and metabolism
Authors: Bussi Claudio , Lai Rachel , Athanasiadi Natalia , Gutierrez Maximiliano G. 
Published: July 2024

ABSTRACT

In vitro
studies are crucial for our understanding of the human macrophage immune functions. However, traditional
in vitro
culture media poorly reflect the metabolic composition of blood, potentially affecting the outcomes of these studies. Here, we analyzed the impact of a physiological medium on human induced pluripotent stem cell (iPSC)-derived macrophages (iPSDM) function. Macrophages cultured in a human plasma-like medium (HPLM) were more permissive to
Mycobacterium tuberculosis
(Mtb) replication and showed decreased lipid metabolism with increased metabolic polarization. Functionally, we discovered that HPLM-differentiated macrophages showed different metabolic organelle content and activity. Specifically, HPLM-differentiated macrophages displayed reduced lipid droplet and peroxisome content, increased lysosomal proteolytic activity, and increased mitochondrial activity and dynamics. Inhibiting or inducing lipid droplet formation revealed that lipid droplet content is a key factor influencing macrophage permissiveness to Mtb. These findings underscore the importance of using physiologically relevant media
in vitro
for accurately studying human macrophage function.

IMPORTANCE

This work compellingly demonstrates that the choice of culture medium significantly influences
M. tuberculosis
replication outcomes, thus emphasizing the importance of employing physiologically relevant media for accurate in vitro host-pathogen interaction studies. We anticipate that our work will set a precedent for future research with clinical relevance, particularly in evaluating antibiotic efficacy and resistance
in cellulo
.
Revised geologic map and structural interpretation of the Mineral King pendant, southern Sierra Nevada, California (USA): Evidence for kilometer-scale folding and structural imbrication of a Permian to mid-Cretaceous volcanosedimentary assemblage
Authors: Greene David C. , Lackey Jade Star , Klemetti Erik W. 
Published: July 2024

Abstract
The Mineral King pendant is an ~15-km-long, northwest-striking assemblage of Permian to mid-Cretaceous metavolcanic and metasedimentary rocks that form a steeply dipping wall-rock screen between large mid-Cretaceous plutons of the Sierra Nevada batholith (California, USA). Pendant rocks are generally well layered and characterized by northwest-striking, steeply dipping, layer-parallel cleavage and flattening foliation and steeply northwest-plunging stretching lineation. Northwest-elongate lithologic units with well-developed parallel layering and an absence of prominent faults or shear zones suggests a degree of stratigraphic continuity. However, U-Pb zircon dating of felsic metavolcanic and volcanosedimentary rocks across the pendant indicates a complex pattern of structurally interleaved units with ages ranging from 277 Ma to 101 Ma.
We utilize a compilation of 39 existing and new U-Pb zircon ages and four reported fossil localities to construct a revised geologic map of the Mineral King pendant that emphasizes age relationships rather than lithologic or stratigraphic correlations as in previous studies. We find that apparently coherent lithologic units are lensoidal and discontinuous and are cryptically interleaved at meter to kilometer scales. Along-strike facies changes and depositional unconformities combine with kilometer-scale tight folding and structural imbrication to create a complex map pattern with numerous discordant units.
Discrete faults or major shear zones are not readily apparent in the pendant, although such structures are necessary to produce the structural complications revealed by our new mapping and U-Pb dating. We interpret the Mineral King pendant to be structurally imbricated by a combination of kilometer-scale tight to isoclinal folding and cryptic faulting, accentuated by, and eventually obscured by, pervasive flattening and vertical stretching that preceded and accompanied emplacement of the bounding mid-Cretaceous plutons. Deformation in the Mineral King pendant represents a significant episode of pure-shear-dominated transpression between ca. 115 Ma and 98 Ma that adds to growing evidence for a major mid-Cretaceous transpressional orogenic event affecting the western U.S. Cordillera.
Cancer screening attendance rates in transgender and gender-diverse patients: a systematic review and meta-analysis
Authors: Chan Alvina , Jamieson Charlotte , Draper Hannah , O'Callaghan Stewart , Guinn Barbara-ann 
Published: July 2024

ObjectivesTo examine disparities in attendance rates at cancer screening services between transgender and gender-diverse (TGD) people in comparison with their cisgender (CG) counterparts, and to determine whether these differences were based on the anatomical organ screened.DesignSystematic review and meta-analysis.Data sourcesPubMed, EMBASE (via Ovid), CINAHL Complete (via EBSCO) and Cochrane Library from inception to 30 September 2023.MethodsStudies for inclusion were case-control or cross-sectional studies with quantitative data that investigated TGD adults attending any cancer screening service. Exclusion criteria were studies with participants who were ineligible for cancer screening or without samples from TGD individuals, qualitative data and a cancer diagnosis from symptomatic presentation or incidental findings. A modified Newcastle-Ottawa Scale was used to assess risk of bias, during which seven reports were found incompatible with the inclusion criteria and excluded. Results were synthesised through random-effects meta-analysis and narrative synthesis.ResultsWe identified 25 eligible records, of which 18 were included in the analysis. These were cross-sectional studies, including retrospective chart reviews and survey analyses, and encompassed over 14.8 million participants. The main outcomes measured were up-to-date (UTD) and lifetime (LT) attendance. Meta-analysis found differences for UTD cervical (OR 0.37, 95% CI 0.23 to 0.60, p<0.0001) and mammography (OR 0.41, 95% CI 0.20 to 0.87, p=0.02) but not for prostate or colorectal screening. There were no meaningful differences seen in LT attendance based on quantitative synthesis. Narrative synthesis of the seven remaining articles mostly supported the meta-analysis. Reduced rates of screening engagement in TGD participants were found for UTD cervical and mammography screening, alongside LT mammography screening.ConclusionsCompared with their CG counterparts, TGD individuals had lower rates of using cervical and mammography screening at the recommended frequencies but displayed similar prevalences of LT attendance. The greatest disparity was seen in UTD cervical screening. Limitations of this review included high risk of bias within studies, high heterogeneity and a lack of resources for further statistical testing. Bridging gaps in healthcare to improve cancer screening experiences and outcomes will require consolidated efforts including working with the TGD community.PROSPERO registration numberCRD42022368911.