bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human platelet microRNA-mRNA networks associated with age and gender revealed by integrated plateletomics
Authors: Simon Lukas M. , Edelstein Leonard C. , Nagalla Srikanth , Woodley Angela B. , Chen Edward S. , Kong Xianguo , Ma Lin , Fortina Paolo , Kunapuli Satya , Holinstat Michael , McKenzie Steven E. , Dong Jing-fei , Shaw Chad A. , Bray Paul F. 
Published: February 2014

Key Points
Unique dataset of human platelet mRNA, miRNA, and physiology reveals mRNAs and miRNAs that differ by age and gender. Interactive public web tool (www.plateletomics.com) provides biologic insights into platelet function and gene expression.
The CtrA phosphorelay integrates differentiation and communication in the marine alphaproteobacterium Dinoroseobacter shibae
Authors: Wang Hui , Ziesche Lisa , Frank Oliver , Michael Victoria , Martin Madeleine , Petersen Jörn , Schulz Stefan , Wagner-Döbler Irene , Tomasch Jürgen 
Published: February 2014

AbstractBackgroundDinoroseobacter shibae,a member of theRoseobacterclade abundant in marine environments, maintains morphological heterogeneity throughout growth, with small cells dividing by binary fission and large cells dividing by budding from one or both cell poles. This morphological heterogeneity is lost if the quorum sensing (QS) system is silenced, concurrent with a decreased expression of the CtrA phosphorelay, a regulatory system conserved inAlphaproteobacteriaand the master regulator of theCaulobacter crescentuscell cycle. It consists of the sensor histidine kinase CckA, the phosphotransferase ChpT and the transcriptional regulator CtrA. Here we tested if the QS induced differentiation ofD. shibaeis mediated by the CtrA phosphorelay.ResultsMutants forctrA,chpTandcckAshowed almost homogeneous cell morphology and divided by binary fission. ForctrAandchpT, expression intranson a plasmid caused the fraction of cells containing more than two chromosome equivalents to increase above wild-type level, indicating that gene copy number directly controls chromosome number. Transcriptome analysis revealed that CtrA is a master regulator for flagellar biosynthesis and has a great influence on the transition to stationary phase. Interestingly, the expression of the autoinducer synthase genesluxI2andluxI3was strongly reduced in all three mutants, resulting in loss of biosynthesis of acylated homoserine-lactones with C14 side-chain, but could be restored by expressing these genes intrans. Several phylogenetic clusters ofAlphaproteobacteriarevealed a CtrA binding site in the promoters of QS genes, includingRoseobactersandRhizobia.ConclusionsThe CtrA phosphorelay induces differentiation of a marineRoseobacterstrain that is strikingly different from that ofC. crescentus. Instead of a tightly regulated cell cycle and a switch between two morphotypes, the morphology and cell division ofDinoroseobacter shibaeare highly heterogeneous. We discovered for the first time that the CtrA phosphorelay controls the biosynthesis of signaling molecules. Thus cell-cell communication and differentiation are interlinked in this organism. This may be a common strategy, since we found a similar genetic set-up in other species in the ecologically relevant group ofAlphaproteobacteria. D. shibaewill be a valuable model organism to study bacterial differentiation into pleomorphic cells.
Somatostatin and its 2A Receptor in Dorsal Root Ganglia and Dorsal Horn of Mouse and Human: Expression, Trafficking and Possible Role in Pain
Authors: Shi Tie-Jun Sten , Xiang Qiong , Zhang Ming-Dong , Barde Swapnali , Kai-Larsen Ylva , Fried Kaj , Josephson Anna , Glück Laura , Deyev Sergey M , Zvyagin Andrei V , Schulz Stefan , Hökfelt Tomas 
Published: February 2014

Background: Somatostatin (SST) and some of its receptor subtypes have been implicated in pain signaling at the spinal level. In this study we have investigated the role of SST and its sst2A receptor (sst2A) in dorsal root ganglia (DRGs) and spinal cord. Results: SST and sst2A protein and sst2 transcript were found in both mouse and human DRGs, sst2A-immunoreactive (IR) cell bodies and processes in lamina II in mouse and human spinal dorsal horn, and sst2A-IR nerve terminals in mouse skin. The receptor protein was associated with the cell membrane. Following peripheral nerve injury sst2A-like immunoreactivity (LI) was decreased, and SST-LI increased in DRGs. sst2A-LI accumulated on the proximal and, more strongly, on the distal side of a sciatic nerve ligation. Fluorescence-labeled SST administered to a hind paw was internalized and retrogradely transported, indicating that a SST-sst2A complex may represent a retrograde signal. Internalization of sst2A was seen in DRG neurons after systemic treatment with the sst2 agonist octreotide (Oct), and in dorsal horn and DRG neurons after intrathecal administration. Some DRG neurons co-expressed sst2A and the neuropeptide Y Y1 receptor on the cell membrane, and systemic Oct caused co-internalization, hypothetically a sign of receptor heterodimerization. Oct treatment attenuated the reduction of pain threshold in a neuropathic pain model, in parallel suppressing the activation of p38 MAPK in the DRGs Conclusions: The findings highlight a significant and complex role of the SST system in pain signaling. The fact that the sst2A system is found also in human DRGs and spinal cord, suggests that sst2A may represent a potential pharmacologic target for treatment of neuropathic pain.
Chemical composition and Biological studies of Ficus benjamina
Authors: Imran Muhammad , Rasool Nasir , Rizwan Komal , Zubair Muhammad , Riaz Muhammad , Zia-Ul-Haq Muhammad , Rana Usman Ali , Nafady Ayman , Jaafar Hawa ZE 
Published: February 2014

Abstract

Background
Current study has been designed to estimate the possible antioxidant, antimicrobial and hemolytic potential of Ficus benjamina different parts (leaves, stem and root).

Results
All examined extracts and fractions were significantly rich in antioxidants and exhibited potent antimicrobial activity. GC/MS analysis of essential oil identified four compounds in stem and eight compounds in root, respectively. HPLC analysis indicated four phenolic compounds (chlorogenic, p-coumaric, ferulic and syringic acids) in roots, three (chlorogenic p-coumaric and ferulic acids) in stem and only one (caffeic acid) in leaves. Extracts of all three parts of F. benjamina exhibited substantial hemolytic activity.

Conclusions
Considering these results, it is concluded that F. benjamina can be used as a potential source for the exploration of new antioxidant compounds and antimicrobial agents.
Foot kinematics in walking on a level surface and on stairs in patients with hallux rigidus before and after cheilectomy
Authors: Kuni Benita , Wolf Sebastian Immanuel , Zeifang Felix , Thomsen Marc 
Published: February 2014

AbstractBackgroundWalking down stairs is a clinically relevant daily activity for older persons. The aim of this pilot study was to investigate the impact of cheilectomy on walking on level ground and on stairs.Methods3D motion analysis of foot kinematics was performed in eight patients with hallux rigidus and 11 healthy control participants with a 12‐camera system, using the Heidelberg foot measurement method before and one year after surgery. The clinical results were documented using the AOFAS Scale.ResultsThe range of motion of the first metatarsophalangeal joint did not improve after the operation under any gait condition. Preoperatively, hallux dorsi‐/plantarflexion in level walking was 11.9° lower in patients than in controls (p = 0.006), postoperatively 14.5° lower (p = 0.004). Comparing walking conditions in patients, hallux dorsi‐/plantarflexion was significantly higher in level walking than in climbing stairs (difference up stairs – level: ‐8.1°, p = 0.018).The AOFAS Scale improved significantly from 56.9 ± 19.9 points (mean ± SD), preoperatively, to 75.9 ± 13.9 points, postoperatively (p = 0.027).ConclusionsCheilectomy is appropriate for reducing symptoms of hallux rigidus. However, neither a positive influence on the range of motion in walking on level ground and on stairs nor a functional improvement was observed in this group of patients.Trial registrationNCT01804491
Brain-derived neurotrophic factor, but not body weight, correlated with a reduction in depression scale scores in men with metabolic syndrome: a prospective weight-reduction study
Authors: Lee I-Te , Fu Chia-Po , Lee Wen-Jane , Liang Kae-Woei , Lin Shih-Yi , Wan Chu-Jen , Sheu Wayne Huey-Herng 
Published: February 2014

Abstract

Background
Obesity, a critical component of metabolic syndrome (MetS), is associated with depression. Deficiency of brain-derived neurotrophic factor (BDNF) is involved in the mechanism of depression. We hypothesized that weight reduction would improve depressive symptoms via increasing BDNF levels in obese men.

Methods
Male adults with obesity were enrolled in a weight-reduction program for twelve weeks. All subjects underwent daily caloric restriction and an exercise program which was regularly assessed in group classes. Fasting blood samples and Zung Self-Rating Depression Scale (Zung SDS) scores were collected for assessments before and after the study.

Results
A total of 36 subjects completed this program. The average reduction in body weight was 8.4 ± 5.1 kg (8.8 ± 5.1%, P < 0.001). Fasting serum BDNF significantly increased after the study (from 40.4 ± 7.8 to 46.9 ± 8.9 ng/ml, P < 0.001). However, the depression symptoms, as assessed by the Zung Self-Rating Depression Scale (Zung SDS), did not reduce significantly (P = 0.486). Divided into subgroups based on changes in BDNF, Zung SDS scores were significantly reduced in subjects with greater BDNF increase than in those with minor BDNF change (-3.9 ± 6.2 vs. 2.3 ± 6.7, P = 0.009). The increased percentage of BDNF was inversely correlated with the change in Zung SDS (r = -0.380, P = 0.022). Multivariate regression analysis showed that reduction in BDNF was independently associated with change in Zung SDS (95% confidence interval -0.315 to -0.052, P = 0.008).

Conclusion
Zung SDS only significantly improved in men with increased fasting BDNF levels after a lifestyle intervention.

Trial registration

(NCT01065753,ClinicalTrials.gov)
Will women soon outperform men in open-water ultra-distance swimming in the ‘Maratona del Golfo Capri-Napoli’?
Authors: Rüst Christoph Alexander , Lepers Romuald , Rosemann Thomas , Knechtle Beat 
Published: February 2014

Abstract
This study investigated the change in sex differences across years in ultra-distance swimming performances at the 36-km ‘Maratona del Golfo Capri-Napoli’ race held from 1954 to 2013. Changes in swimming performance of 662 men and 228 women over the 59-year period were investigated using linear, non-linear and hierarchical regression analyses. Race times of the annual fastest swimmers decreased linearly for women from 731 min to 391 min (r

2
= 0.60, p < 0.0001) and for men from 600 min to 373 min (r

2
= 0.30, p < 0.0001). Race times of the annual top three swimmers decreased linearly between 1963 and 2013 for women from 736.8 ± 78.4 min to 396.6 ± 4.5 min (r

2
= 0.58, p < 0.0001) and for men from 627.1 ± 34.5 min to 374.1 ± 0.3 min (r

2
= 0.42, p < 0.0001). The sex difference in performance for the annual fastest decreased linearly from 39.2% (1955) to 4.7% (2013) (r

2
= 0.33, p < 0.0001). For the annual three fastest competitors, the sex difference in performance decreased linearly from 38.2 ± 14.0% (1963) to 6.0 ± 1.0% (2013) (r

2
= 0.43, p < 0.0001). In conclusion, ultra-distance swimmers improved their performance at the ‘Maratona del Golfo Capri-Napoli’ over the last ~60 years and the fastest women reduced the gap with the fastest men linearly from ~40% to ~5-6%. The linear change in both race times and sex differences may suggest that women will be able to achieve men’s performance or even to outperform men in the near future in an open-water ultra-distance swimming event such as the ‘Maratona del Golfo Capri-Napoli’.
Nanoleakage in indirect composite restorations using different combinations of resin coating technique
Authors: Medina Alberth David Correa , de Paula Andréia Bolzan , Naufel Fabiana Scarparo , Puppin-rontani Regina Maria , Correr-Sobrinho Lourenço , Sinhoreti Mário Alexandre Coelho 
Published: February 2014

Abstract
The aim of this study was to evaluate the nanoleakage patterns in indirect composite restorations bonding to dentin using different combinations of Resin Coating, after thermal and load cycling. Twenty five extracted third molars were used in the study; two box-like Class II cavities were prepared in each tooth (mesial and distal surface). The 50 cavities were distributed in 5 groups according to the RC materials combinations: G1: Etch-rinse 2 steps/Hydrophobic monomer; G2: Etch-rinse 2steps/Flow composite-resin, G3: Self-etch 1step, G4: Self-etch1step/Flowable composite resin liner, G5: Self-etch 2step/Flowable composite-resin liner. The cavities were molded with a vinyl polysiloxane impression material and the molds were poured with stone plaster. The fillings were confectioned using the Sinfony composite system (3 M/ESPE) and cemented with resin luting cement (Rely X ARC). After 24 hours, the teeth were submitted to thermocycling (2000 cycles, 5° to 55°C) and load cycling (250,000 cycles, 30 N). Past 24hours, the restored teeth were sectioned into serial slabs and immersed in 50% ammoniacal silver nitrate for 24 h, exposed to photo-developing solution for 8 h, carbon coated and observed in SEM using backscattered electron mode. Diverse nanoleakage patterns were observed for the different RC combinations. Silver accumulation were observed in the entire thickness of the hybrid layer in G1 and G2 groups, while in the “all in one” self-etch groups (G3 and G4) silver accumulations similar to “water trees” within the adhesive layer were observed. In G5 group, less nanoleakage than other groups was observed and it was limited to the hybrid layer. The group G5 presented a superior behavior and revealed less nanoleakage compared to the other groups.
Relative Value Health Insurance
Authors: Korobkin Russell 
Published: February 2014

AbstractIncreases in health costs continue to outpace general inflation, and implementation of the Patient Protection and Affordable Care Act will exacerbate the problem by adding more Americans to the ranks of the insured. The most commonly proposed solutions — bureaucratic controls, greater patient cost sharing, and changes to physician incentives — all have substantial weaknesses. This article proposes a new paradigm for rationalizing health care expenditures called “relative value health insurance,” a product that would enable consumers to purchase health insurance that covers cost-effective treatments but excludes cost-ineffective treatments. A combination of legal and informational impediments prevents private insurers from marketing this type of product today, but creative use of comparative effectiveness research, funded as a part of health care reform, could make relative value health insurance possible. Data deficits, adverse selection risks, and heterogeneous values among consumers create obstacles to shifting the health insurance system to this paradigm, but they could be overcome.
Dysregulated interactions between lamin A and SUN1 induce abnormalities in the nuclear envelope and endoplasmic reticulum in progeric laminopathies
Authors: Chen Zi-Jie , Wang Wan-Ping , Chen Yu-Ching , Wang Jing-Ya , Lin Wen-Hsin , Tai Lin-Ai , Liou Gan-Guang , Yang Chung-Shi , Chi Ya-Hui 
Published: February 2014

ABSTRACT
Hutchinson-Gilford progeria syndrome (HGPS) is a human progeroid disease caused by a point mutation on the LMNA gene. We reported previously that the accumulation of the nuclear envelope protein SUN1 contributes to HGPS nuclear aberrancies. However, the mechanism by which interactions between mutant lamin A (also known as progerin or LAΔ50) and SUN1 produce HGPS cellular phenotypes requires further elucidation. Using light and electron microscopy, this study demonstrated that SUN1 contributes to progerin-elicited structural changes in the nuclear envelope and the endoplasmic reticulum (ER) network. We further identified two domains through which full-length lamin A associates with SUN1, and determined that the farnesylated cysteine within the CaaX motif of lamin A has a stronger affinity for SUN1 than does the lamin A region containing amino acids 607 to 656. Farnesylation of progerin enhanced its interaction with SUN1 and reduced SUN1 mobility, thereby promoting the aberrant recruitment of progerin to the ER membrane during postmitotic assembly of the nuclear envelope, resulting in the accumulation of SUN1 over consecutive cellular divisions. These results indicate that the dysregulated interaction of SUN1 and progerin in the ER during nuclear envelope reformation determines the progression of HGPS.
Theoretical Investigation on the Electronic Transport Properties of Iron(II) Porphyrin for CO Sensing with Single-walled Carbon Nanotubes
Authors: He Yuanyuan , Zhang Jinjiang , Zhao Jianwei 
Published: February 2014

Abstract
We studied the electronic transport properties of a new type of iron(II) porphyrin (PP-Fe) chemosensor for CO with two single-walled carbon nanotube (SWCNT) electrodes, using the first-principle density functional theory. Owing to the fact that electron does not pass through the Fe center when the CO molecule is absorbed to PP-Fe, leading to a considerable difference between the I–V curves of PP-Fe before and after CO absorption, it has great potential for application as a chemical sensor for CO.
Great uncertainty regarding treatment of varicose vein recurrence
Authors: Nelzén O. 
Published: February 2014

SummaryIntroduction: Although varicose vein recurrence is common and 10–30 % of all varicose vein surgery is done for recurrence of some sort, there are very few studies that can guide us to the best re-treatment option. With the introduction of minimal invasive endovenous treatments there is a variety of possible options besides traditional open surgical techniques.Method: The Scandinavian Venous Forum held a symposium at the GSP meeting in Lü-beck 2012 and this review article is based on data from the presentations at that symposium. Further data has been added regarding new knowledge that was not available a year ago, from PubMed search and article references.Results: The most common reasons for recurrence are discussed and also the discrepancy between neovascularization (NV) and recurrence due to technical failures. It is likely that NV is the most commonly duplex detected type of recurrence following open groin surgery, less common early after endovascular techniques. However, technical or tactical failures are the most common reasons for redo surgery because of symptomatic recurrence. NV seldom leads to symptomatic recurrences and thus a need for re- treatment. There is a risk that the stumps left following endovenous treatments will become a source for symptomatic recurrence after 5–10 years and indications of that have been reported in the few available 5 year RCT-reports following laser treatments. Treatment of recurrence due to stumps in the groin can be done safely within a reasonable operating time through a medial approach and the stump itself can generally not be treated with any of the endovenous alternative methods. Foam treatment can be used for most other recurrent veins but the durability is unknown. Endovascular thermal ablation can only be used for reopened or remaining saphenous veins and accessory saphenous veins while tributaries have to be treated by stab excisions or foam.Conclusion: Long term reports of results of redo surgery are limited but suggest reasonably good results from open surgical intervention and are non-existent for the endovenous techniques. So far groin recurrence seems best treated surgically by an indirect approach, preferably medial. More studies are needed to find the best treatment regime for varicose vein recurrence in general and hybrid procedures might be the way forward by combinations of different techniques.
Nominal Group Technique consultation of a Pulmonary Rehabilitation Programme
Authors: Hutchings Hayley A , Rapport Frances L , Wright Sarah , Doel Marcus A , Clement Clare , Lewis Keir E 
Published: February 2014

Objective:The purpose of the study was to determine what patients, professionals and significant others regarded as the most important positive- and challenging aspects of Pulmonary Rehabilitation Programmes for patients with Chronic Obstructive Pulmonary Disease (COPD) and to gain insight into how such programmes could be developed and improved.Method:A modified Nominal Group Technique method was used in three consultation workshops (one with COPD patients who had recently undertaken a Pulmonary Rehabilitation Programme; one with ‘significant others’ of the same patients; one with secondary care professionals who deliver the Pulmonary Rehabilitation Programme).Results:Each of three workshops resulted in the production of approximately ten positive- and ten challenging aspects related to Pulmonary Rehabilitation Programmes. These were further developed by a process of thematisation into seven broad themes. The most important was ‘the patient’, followed by ‘physical health’; jointly ranked as third were: ‘mental health’ and ‘knowledge and education’. ‘The programme’ and ‘professional characteristics’ were jointly ranked as fifth, with ‘the future’ being ranked as the least important theme.Conclusions:The modified Nominal Group Technique method allowed the development of a ranked thematic list that illustrated the important positive- and challenging aspects of Pulmonary Rehabilitation Programmes for patients with COPD. These themes should be core to planning future Pulmonary Rehabilitation Programmes, particularly if patients and carer views are to be considered.
KEGGViewer, a BioJS component to visualize KEGG Pathways
Authors: Villaveces Jose M. , Jimenez Rafael C. , Habermann Bianca H. 
Published: February 2014

Summary: Signaling pathways provide essential information on complex regulatory processes within the cell. They are moreover widely used to interpret and integrate data from large-scale studies, such as expression or functional screens. We present KEGGViewer a BioJS component to visualize KEGG pathways and to allow their visual integration with functional data. Availability: KEGGViewer is an open-source tool freely available at the BioJS Registry. Instructions on how to use the tool are available at http://goo.gl/dVeWpg and the source code can be found at http://github.com/biojs/biojs and DOI:10.5281/zenodo.7708.
PsicquicGraph, a BioJS component to visualize molecular interactions from PSICQUIC servers
Authors: Villaveces Jose M. , Jimenez Rafael C. , Habermann Bianca H. 
Published: February 2014

Summary: Protein interaction networks have become an essential tool in large-scale data analysis, integration, and the visualization of high-throughput data in the context of complex cellular networks. Many individual databases are available that provide information on binary interactions of proteins and small molecules. Community efforts such as PSICQUIC aim to unify and standardize information emanating from these public databases. Here we introduce PsicquicGraph, an open-source, web-based visualization component for molecular interactions from PSIQUIC services. Availability: PsicquicGraph is freely available at the BioJS Registry for download and enhancement. Instructions on how to use the tool are available here http://goo.gl/kDaIgZ and the source code can be found at http://github.com/biojs/biojs and DOI:10.5281/zenodo.7709.