bluuebunny/crossref_metadata_2025_split huggingface dataset:
AFAP1L1, a novel associating partner with vinculin, modulates cellular morphology and motility, and promotes the progression of colorectal cancers
Authors: Takahashi Ryo , Nagayama Satoshi , Furu Moritoshi , Kajita Yoichiro , Jin YongHui , Kato Tomohisa , Imoto Seiya , Sakai Yoshiharu , Toguchida Junya 
Published: April 2014

AbstractWe have previously identified actin filament‐associated protein 1‐like 1 (AFAP1L1) as a metastasis‐predicting marker for spindle cell sarcomas by gene expression profiling, and demonstrated that AFAP1L1 is involved in the cell invasion process by in vitro analyses. However, its precise molecular function has not been fully elucidated, and it remains unknown whether AFAP1L1 could be a prognostic marker and/or therapeutic target of other malignancies. In this study, we found a marked elevation of AFAP1L1 gene expression in colorectal cancer (CRC) tissues as compared to the adjacent normal mucosa. Multivariate analysis revealed that AFAP1L1 was an independent and significant factor for the recurrence of rectal cancers. Moreover, the addition of the AFAP1L1 expression level to the lymph node metastasis status provided more predictive information regarding postoperative recurrence in rectal cancers. AFAP1L1‐transduced CRC cells exhibited a rounded shape, increased cell motility on planar substrates, and resistance to anoikis in vitro. AFAP1L1 localized to the ringed structure of the invadopodia, together with vinculin, and AFAP1L1 was identified as a novel associating partner of vinculin by immunoprecipitation assay. AFAP1L1‐transduced cells showed accelerated tumor growth in vivo, presumably reflecting the anoikis resistance of these AFAP1L1‐expressing cells. Furthermore, the local administration of a siRNA against AFAP1L1 significantly suppressed the in vivo tumor growth of xenografts, suggesting that AFAP1L1 might be a candidate therapeutic target for CRCs. These results suggest that AFAP1L1 plays a role in the progression of CRCs by modulating cell shape and motility and by inhibiting anoikis, presumably through interactions with vinculin‐including protein complexes.
Molecular testing in lung cancer: Fine‐needle aspiration specimen adequacy and test prioritization prior to the CAP/IASLC/AMP Molecular Testing Guideline publication
Authors: Rafael Oana C. , Aziz Mohamed , Raftopoulos Harry , Vele Oana E. , Xu Weisheng , Sugrue Chiara 
Published: April 2014

BACKGROUNDSubtyping of lung carcinoma with immunohistochemistry is essential for diagnosis, whereas molecular testing (MT) is required for therapy guidance. In the current study, the authors report on MT performed on fine‐needle aspiration specimens at the study institution over a 2‐year period preceding the April 2013 College of American Pathologists (CAP)/International Association for the Study of Lung Cancer (IASLC)/Association for Molecular Pathology (AMP) Molecular Testing Guideline (MTG) publication.METHODSThe database of the study institution was retrospectively queried for cases of lung and thoracic/lower cervical lymph node fine‐needle aspiration specimens for 2011 through 2012.RESULTSOf 246 selected cases, 26 featured a limited amount of material in cell blocks. MT increased significantly between 2011 and 2012 and was requested in 39.4% of cases (97 of 246 cases): 86 of those cases had at least 1 MT result and 11 had insufficient material for any MT. Anaplastic lymphoma kinase (ALK) testing was performed in 9 cases in which DNA was insufficient for epidermal growth factor receptor (EGFR) testing. In addition, 13 cases of adenocarcinoma/non‐small cell lung carcinoma had at least 1 MT canceled because of insufficient DNA, but at the same time had an average of 3.46 immunohistochemical stains performed.CONCLUSIONSOf all the cytology specimens, 10.6% featured limited material; however, no universally accepted testing sequence priority was available at the time the study was performed. As per the MTG, MT should take precedence over immunohistochemistry in cases of adenocarcinoma/non‐small cell lung carcinoma. Approximately 5.3% of the specimens in the current study had insufficient material for MT while having multiple stains performed instead. The MTG also recommend performing EGFR before ALK testing; the authors found 9 cases with insufficient material for EGFR testing that had ALK testing performed. The results of the current study underscore the need for a testing prioritization algorithm in view of the MTG publication to serve as reference for both clinicians and pathologists. Cancer (Cancer Cytopathol) 2014;122:454–458. © 2014 American Cancer Society.
Strategy for analysis of flow diverting devices based on multi‐modality image‐based modeling
Authors: Cebral Juan R. , Mut Fernando , Raschi Marcelo , Ding Yong‐Hong , Kadirvel Ramanathan , Kallmes David 
Published: April 2014

SUMMARYQuantification and characterization of the hemodynamic environment created after flow diversion treatment of cerebral aneurysms is important to understand the effects of flow diverters and their interactions with the biology of the aneurysm wall and the thrombosis process that takes place subsequently. This paper describes the construction of multi‐modality image‐based subject‐specific CFD models of experimentally created aneurysms in rabbits and subsequently treated with flow diverters. Briefly, anatomical models were constructed from 3D rotational angiography images, flow conditions were derived from Doppler ultrasound measurements, stent models were created and virtually deployed, and the results were compared with in vivo digital subtraction angiography and Doppler ultrasound images. The models were capable of reproducing in vivoobservations, including velocity waveforms measured in the parent artery, peak velocity values measured in the aneurysm, and flow structures observed with digital subtraction angiography before and after deployment of flow diverters. The results indicate that regions of aneurysm occlusion after flow diversion coincide with slow and smooth flow patterns, whereas regions still permeable at the time of animal sacrifice were observed in parts of the aneurysm exposed to larger flow activity, that is, higher velocities, more swirling, and more complex flow structures. Copyright © 2014 John Wiley & Sons, Ltd.
Challenges of “Going Nano”: Enhanced Electrochemical Performance of Cobalt Oxide Nanoparticles by Carbothermal Reduction and In Situ Carbon Coating
Authors: Bresser Dominic , Paillard Elie , Niehoff Philip , Krueger Steffen , Mueller Franziska , Winter Martin , Passerini Stefano 
Published: April 2014

AbstractThe electrochemical performance of nano‐ and micron‐sized Co3O4 is investigated, highlighting the substantial influence of the specific surface area on the obtainable specific capacities as well as the cycling stability. In fact, Co3O4 materials with a high surface area (i.e. a small particle size) show superior specific features, which are, however, accompanied by a rapid capacity fading, owing to the increased formation of an insulating polymeric surface film that results from transition‐metal‐catalyzed electrolyte decomposition. The simultaneous coating with carbon of Co3O4 nanoparticles and in situ reduction of the Co3O4 by a carbothermal route yields a CoOCoC nanocomposite. The formation of this material substantially enhances the long‐term cycling stability and coulombic efficiency of the lithium‐ion active material used. Although the metallic cobalt enhances the electronic conductivity within the electrode and remains electrochemically inactive (as revealed by in situ powder X‐ray diffraction analysis), it might have a detrimental effect on the long‐term cycling stability by catalytically inducing continuous electrolyte decomposition.
Molecular Conductors with Effectively Half‐Filled Electronic States Based on Tetrathiafulvalene Derivatives Condensed with a 2‐Isopropylidene‐1,3‐dithiole Ring
Authors: Furuta Keisuke , Kohno Shuhei , Shirahata Takashi , Misaki Yohji 
Published: April 2014

AbstractThe electron donor 2‐isopropylidene‐1,3‐dithiolo[4,5‐d]‐4,5‐ethylenediselenotetrathiafulvalene (3) provides two new molecular conductors, (3)2[M(CN)2] (M = Ag and Au). These salts crystallize in the triclinic P$\bar {1}$ space group. The donor sheet structure of these salts is the so‐called β‐type molecular array, in which the donor molecules form a head‐to‐tail dimer. The formation of considerable dimerization and the 2:1 donor–anion ratio result in an effectively half‐filled electronic nature. These salts show metallic conducting behavior from room temperature. The metallic conducting behavior of (3)2[Ag(CN)2] was retained down to 8 K; however, the resistivity of (3)2[Au(CN)2] increased at around 50 K. The magnetic susceptibility of (3)2[Au(CN)2] suggests that the strength of the electron correlation for this salt is enhanced below 50 K.
Charge Disproportionation and Magnetoresistivity in a Double Perovskite with Alternate Fe4+(d4) and Mn4+(d3) Layers
Authors: Ganesanpotti Subodh , Tassel Cédric , Hayashi Naoaki , Goto Yoshihiro , Bouilly Guillaume , Yajima Takeshi , Kobayashi Yoji , Kageyama Hiroshi 
Published: April 2014

AbstractAn oxygen‐stoichiometric, B‐site‐ordered perovskite Ca2Fe1.1Mn0.9O6 (CFMO) with alternate stacking of Fe and Mn layers was obtained through topochemical oxidation of the corresponding brownmillerite phase under high pressure in the presence of KClO4. The structure crystallizes in the P21/m space group with a doubling of the cell along all three crystallographic axes. Mössbauer spectroscopy, susceptibility, and resistivity measurements suggest ferromagnetic interactions between Fe4+(d4) and Mn4+(d3) along [001] through a double‐exchange mechanism, a situation similar to half‐doped manganese perovskite oxides. Upon cooling, CFMO exhibits a ferrimagnetic transition below Tc = 90 K, likely accompanied by a charge disproportionation of the iron site, 2Fe4+ → Fe3+ + Fe5+. A reasonably good magnetoresistivity of 27 % was observed below Tc.
Synthesis and Absolute Configurations of Six Natural Phenylpropanoids
Authors: Liu Xue‐Lian , Chen Hui‐Jun , Yang Yang‐Yang , Wu Yikang , You Jun 
Published: April 2014

AbstractIn an effort to find answers to the tantalizing questions about the absolute configurations of a group of long‐known natural phenylpropanoids with very similar structures but different signs for the optical rotations, the compounds in question were synthesized in enantiomerically pure form using Evans asymmetric alkylation to generate the stereogenic centres with predefined absolute configurations. The 1H and 13C NMR spectra of the synthetic products were very consistent with those reported for their natural counterparts. In most cases, the optical rotations were also consistent with the corresponding data for the natural samples. The new findings not only allowed unequivocal assignments of the absolute configurations for the natural products, but also revealed that the configurations of closely related compounds from the same plant may be different.
How normative and social identification processes predict self‐determination to engage in derogatory behaviours against outgroup hockey fans
Authors: Amiot Catherine E. , Sansfaçon Sophie , Louis Winnifred R. 
Published: April 2014

AbstractBringing together self‐determination theory, intergroup theories based on the social identity approach, and normative approaches, three studies conducted among hockey fans tested if social norms and social identity predict greater self‐determined motivation to engage in derogatory behaviours against an outgroup team and higher frequency of these behaviours. Higher self‐determination was conceptualised as an indicator of internalisation. In Study 1, hockey fans who identified more strongly as fans of the Montreal Canadiens (N = 181) displayed a stronger positive association between the perceived norm in favour of outgroup derogation and self‐determined motivation to engage in derogatory behaviours against fans of an outgroup team. This interaction also emerged on the frequency with which the derogatory behaviours were enacted. In Studies 2 and 3 (Ns = 105 and 116), this norm by social identity interaction was replicated on both the self‐determination and the frequency outcomes for fans of a diversity of teams in the National Hockey League. In Study 3, these findings were observed over and above a manipulation that framed derogatory behaviours as being either harmful or beneficial. Results are discussed in light of motivational theories, normative approaches, and intergroup theories based on the social identity approach. Copyright © 2014 John Wiley & Sons, Ltd.
Dielectrophoretic isolation and detection of cancer‐related circulating cell‐free DNA biomarkers from blood and plasma
Authors: Sonnenberg Avery , Marciniak Jennifer Y. , Skowronski Elaine A. , Manouchehri Sareh , Rassenti Laura , Ghia Emanuela M. , Widhopf George F. , Kipps Thomas J. , Heller Michael J. 
Published: April 2014

Conventional methods for the isolation of cancer‐related circulating cell‐free (ccf) DNA from patient blood (plasma) are time consuming and laborious. A DEP approach utilizing a microarray device now allows rapid isolation of ccf‐DNA directly from a small volume of unprocessed blood. In this study, the DEP device is used to compare the ccf‐DNA isolated directly from whole blood and plasma from 11 chronic lymphocytic leukemia (CLL) patients and one normal individual. Ccf‐DNA from both blood and plasma samples was separated into DEP high‐field regions, after which cells (blood), proteins, and other biomolecules were removed by a fluidic wash. The concentrated ccf‐DNA was detected on‐chip by fluorescence, and then eluted for PCR and DNA sequencing. The complete process from blood to PCR required less than 10 min; an additional 15 min was required to obtain plasma from whole blood. Ccf‐DNA from the equivalent of 5 μL of CLL blood and 5 μL of plasma was amplified by PCR using Ig heavy‐chain variable (IGHV) specific primers to identify the unique IGHV gene expressed by the leukemic B‐cell clone. The PCR and DNA sequencing results obtained by DEP from all 11 CLL blood samples and from 8 of the 11 CLL plasma samples were exactly comparable to the DNA sequencing results obtained from genomic DNA isolated from CLL patient leukemic B cells (gold standard).
An Empirical Comparison of Meta‐analysis and Mega‐analysis of Individual Participant Data for Identifying Gene‐Environment Interactions
Authors: Sung Yun Ju , Schwander Karen , Arnett Donna K. , Kardia Sharon L.R. , Rankinen Tuomo , Bouchard Claude , Boerwinkle Eric , Hunt Steven C. , Rao Dabeeru C. 
Published: April 2014

ABSTRACTFor analysis of the main effects of SNPs, meta‐analysis of summary results from individual studies has been shown to provide comparable results as “mega‐analysis” that jointly analyzes the pooled participant data from the available studies. This fact revolutionized the genetic analysis of complex traits through large GWAS consortia. Investigations of gene‐environment (G×E) interactions are on the rise since they can potentially explain a part of the missing heritability and identify individuals at high risk for disease. However, for analysis of gene‐environment interactions, it is not known whether these methods yield comparable results. In this empirical study, we report that the results from both methods were largely consistent for all four tests; the standard 1 degree of freedom (df) test of main effect only, the 1 df test of the main effect (in the presence of interaction effect), the 1 df test of the interaction effect, and the joint 2 df test of main and interaction effects. They provided similar effect size and standard error estimates, leading to comparableP‐values. The genomic inflation factors and the number of SNPs with various thresholds were also comparable between the two approaches. Mega‐analysis is not always feasible especially in very large and diverse consortia since pooling of raw data may be limited by the terms of the informed consent. Our study illustrates that meta‐analysis can be an effective approach also for identifying interactions. To our knowledge, this is the first report investigating meta‐versus mega‐analyses for interactions.
Discoidin domain receptors: A promoter of the aggressive behavior of ameloblastomas
Authors: Yang Jing , Liu Jie , Zhong Ming , Chen Yu , Song Ming , Du Yaming 
Published: April 2014

AbstractDiscoidin domain receptors 1 and 2 (DDR1 and DDR2) are members of the receptor tyrosine kinases, which regulate fundamental cellular processes concerning proliferation, differentiation, adhesion, motility, and apoptosis. The dysregulation of these receptors is linked to a number of human diseases, including fibrotic disorders, atherosclerosis, and cancer. However, there have been no studies that analyzed the expression of these DDRs in ameloblastomas (ABs). In this study, we investigated the expression level and distribution of both DDRs in ABs and determined whether these receptors could predict the prognosis of the disease. Real‐time reverse transcription polymerase chain reaction, western blot, and immunohistochemical analyses were performed to detect the DDR mRNA and protein expression levels in normal oral mucosa (NOM) and ABs. The relationship of the DDRs with the clinicopathology and prognosis of ABs was analyzed statistically. The mRNA expression levels of DDR1 and DDR2 were found to be increased by 3.42‐ and 3.66‐fold in ABs versus NOM, respectively. Recurrent ABs displayed higher DDR mRNA expression than did primary ABs (P < 0.05). Using western blot analysis, the DDR proteins were found to be lower in NOM than in ABs (P < 0.05), and primary ABs showed lower expression levels than did recurrent ones (P < 0.05). Immunohistochemically, the DDR protein expressions were markedly higher in ABs than in NOM (P < 0.05), and AB patients with higher DDR protein expression showed higher recurrence (P < 0.05). Multivariate analysis with the Cox proportional hazards model indicated the expression of both DDRs to be an independent prognostic factor of ABs. It was suggested that the up‐regulation of DDR expression might play an important role in the tumorigenesis and aggressiveness of ABs. Thus, DDR protein expression may be considered as a good biomarker for indicating the prognosis of ABs. © 2014 IUBMB Life, 66(4):292–299, 2014
Evaluation of femoral perfusion in a rabbit model of steroid‐induced osteonecrosis by dynamic contrast‐enhanced mri with a high magnetic field MRI system
Authors: Hayashi Shigeki , Fujioka Mikihiro , Ikoma Kazuya , Saito Masazumi , Ueshima Keiichiro , Ishida Masashi , Kuribayashi Masaaki , Ikegami Akira , Mazda Osam , Kubo Toshikazu 
Published: April 2014

PurposeTo evaluate perfusion during the early phase after steroid administration in vivo using dynamic contrast‐enhanced magnetic resonance imaging (DCE‐MRI) with a high magnetic field MRI system. The main pathogenesis of steroid‐induced osteonecrosis is considered to be ischemia.Materials and MethodsA single dose of methylprednisolone (MPSL) was injected into nine rabbits. DCE‐MRI was performed for these rabbits before MPSL administration and 1, 5, 10, and 14 days after administration. Time–signal intensity curves were created for each femur based on the signal intensity to evaluate perfusion. Enhancement ratio (ER), initial slope (IS), and area under the curve (AUC) were calculated and the value before MPSL administration and the minimal value after administration were compared statistically.ResultsER, IS, and AUC values after MPSL administration significantly decreased (P < 0.05, P < 0.01, and P < 0.01, respectively). All of them decreased by the 5th day in 56% of the femora and by the 14th day in 83%, and some femora even showed a decrease from the 1st day.ConclusionIn this study, decreased perfusion in the femora after steroid administration was proven. Additionally, we could show that it occurred from the early days after steroid administration. J. Magn. Reson. Imaging 2015;41:935–940. © 2014 Wiley Periodicals, Inc.
Status Quo and Perspectives of Laser Micromachining
Authors: Mayerhofer Roland , Geiger Stephan 
Published: April 2014

AbstractThe global laser system market increased six‐fold over the past two decades as laser material processing successfully took over domains formerly occupied by conventional techniques. Today, global key markets like mobile communication, healthcare or transportation are pushing laser micromachining and waiting for it to push the limits of what is technically feasible. Global players will look for laser makers with global reach to secure immediate support wherever they manufacture. A diversified product portfolio and a network of specialized partners will help laser manufacturers to offer and evaluate whatever laser technology is needed. Finally, customer demands will push laser makers like Rofin to offer process design, laser source, beam guidance, parts handling, machine building and even certification and validation support from one source.
High Power VCSEL Systems
Authors: Moench Holger , Derra Günther 
Published: April 2014

AbstractNew high power infrared sources in the kilowatt range are based on modular building blocks of LED‐like microlaser arrays. Modules in a very compact form factor enable easy integration in industrial heating processes. Fully flexible and dynamic control of the heating profile is paving the path to digitization of manufacturing processes, here particularly to “Digital Thermal Processing”.
Material Processing with Ultrashort‐pulsed Lasers
Authors: Holtkamp Jens , Eifel Stephan , Ryll Joachim 
Published: April 2014

AbstractThe outstanding properties of ultrashort‐pulsed laser have led to a huge amount of potential applications as a precise and flexible tool for materials processing. On the other hand, complex process parameter correlations paired with high investment costs require efficient process chains to ensure an economical use in production. Pulsar Photonics presents a tool system that stands out by its integrated inspection routines and workflow management. Embedded sensors and networked actuators offer the Industry 4.0 capability of machine data acquisition together with the benefits of an improved reliability. For competitive series applications Pulsar Photonics focuses on an increased efficiency by multiple beam processing. Hereby the laser beam is divided into up to 100 parallelized beams, thus enabling the efficient production of periodic structures, large‐scaled ablation or the parallel processing of multiple components.
Conformational Dynamics of DNA G‐Quadruplex in Solution Studied by Kinetic Capillary Electrophoresis Coupled On‐line with Mass Spectrometry
Authors: Mironov Gleb G. , Okhonin Victor , Khan Nasrin , Clouthier Christopher M. , Berezovski Maxim V. 
Published: April 2014

AbstractInvited for this months cover is the group of Prof. Maxim V. Berezovski. The cover picture shows the two‐dimensional separation of unfolded (green) and folded (red) forms of G‐quadruplex (GQ) DNA. The first dimension is kinetic capillary electrophoresis (KCE) separation of unfolded and folded DNA with different K+ concentrations in solution; the second dimension is ion mobility mass spectrometry separation of DNA conformers in the gas phase. DNA folding into a compact GQ structure is mediated by K+ ions. For more details, see the Full Paper on p. 58 ff.
The Effects of Contraception on Female Poverty
Authors: Browne Stephanie P. , LaLumia Sara 
Published: April 2014

AbstractPoverty rates are particularly high among households headed by single women, and childbirth is often the event preceding these households’ poverty spells. This paper examines the relationship between legal access to the birth control pill and female poverty. We rely on exogenous cross‐state variation in the year in which oral contraception became legally available to young, single women. Using census data from 1960 to 1990, we find that having legal access to the birth control pill by age 20 significantly reduces the probability that a woman is subsequently in poverty. We estimate that early legal access to oral contraception reduces female poverty by 0.5 percentage points, even when controlling for completed education, employment status, and household composition.