bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison between two different in vitro basophil activation tests for gluten- and cow’s milk protein sensitivity in irritable bowel syndrome (IBS)-like patients
Authors: Carroccio Antonio , Brusca Ignazio , Mansueto Pasquale , D’alcamo Alberto , Barrale Maria , Soresi Maurizio , Seidita Aurelio , La Chiusa Stella M. , Iacono Giuseppe , Sprini Delia 
Published: November 2012

Abstract

Background: The diagnosis of food hypersensitivity (FH) in adult patients with gastrointestinal symptoms, beyond the immediate IgE-mediated clinical manifestations, is very often difficult. The aims of our study were to: 1) evaluate the frequency of FH in patients with irritable bowel syndrome (IBS)-like clinical presentation; and 2) compare the diagnostic accuracy of two different methods of in vitro basophil activation tests.

Methods: Three hundred and five patients (235 females, age range 18–66 years) were included and underwent a diagnostic elimination diet and successive double-blind placebo-controlled (DBPC) challenges. Two different methods of in vitro basophil activation tests (BAT) (CD63 expression after in vitro wheat or cow’s milk proteins stimulation) were evaluated: one was performed on separated leukocytes, and the other on whole blood.

Results: Ninety patients of the 305 studied (29.5%) were positive to the challenges and were diagnosed as suffering from FH. BAT on separate leukocytes showed a sensitivity of 86% and a specificity of 91% in FH diagnosis. BAT on whole blood showed a sensitivity of 15%–20% and a specificity of 73% in FH diagnosis (p<0.0001 compared to the other method).

Conclusions: About one third of the IBS patients included in the study were suffering from FH and were cured on the elimination diet. The BAT based on CD63 detection on whole blood samples did not work in FH diagnosis and showed a significantly lower sensitivity, specificity and diagnostic accuracy than the assay based on separated leukocytes.
Genetic defects in folate and cobalamin pathways affecting the brain
Authors: Kirsch Susanne H. , Herrmann Wolfgang , Obeid Rima 
Published: November 2012

Abstract
Folate and cobalamin are necessary for early brain development and function. Deficiency of folate or cobalamin during pregnancy can cause severe malformation in the central nervous system such as neural tube defects. After birth, folate and cobalamin deficiency can cause anemia, failure to thrive, recurrent infections, psychiatric and neurological symptoms. The folate and the homocysteine metabolic pathways interact at a central step where 5-methyltetrahydrofolate donates its methyl group to homocysteine to produce methionine and tetrahydrofolate. Methyl cobalamin and folate interact at this critical step. Both nutrients have a crucial role in DNA synthesis and in delivering S-adenosylmethionine, the universal methyl donor. Severe and mild inherited disorders in folate and cobalamin pathways have been described. The two groups of disorders share some similarities, but differ in the molecular mechanism, metabolic dysregulation, and disease management. This review summarizes selected disorders, including rare and common mutations that affect folate and cobalamin absorption, transport, or dependent enzymes. When the mutations are discovered early enough, many of the described disorders are easily treatable by B vitamin supplementation, which often prevents or reverses the manifestation of the disease. Therefore, the screening for mutations is recommended and should be carried out as early as possible: after occurrence of the first symptoms or when a certain constellations of the folate and cobalamin related markers are measured, such as elevated homocysteine and/or methylmalonic acid.
Sequential amination of heteroaromatic halides with aminopyridine 1 - oxides and their N-protected derivatives based on novel aza-Smiles rearrangement
Authors: Wolińska Ewa 
Published: November 2012

Abstract
The SNAr and Pd-catalyzed amination of chloro derivatives of azines, diazines, and triazines with 2-aminopyridine 1-oxides and their N-protected derivatives was described.
Effect of short-chain silicones bearing different functional groups on the resistance of pine (Pinus sylvestris L.) and beech (Fagus sylvatica L.) against decay fungi
Authors: Pries Malte , Wagner Roland , Kaesler Karl-Heinz , Militz Holger , Mai Carsten 
Published: November 2012

Abstract
Blocks of pine sapwood and beech wood were treated with water-based emulsions containing short-chain silicones with different α-ω-bonded functional groups, such as diamino, carboxyl and carbonyl, betain, and epoxy groups. The weight percent gain upon treatment of the pine wood specimens was relatively high (10%–20%), but their cross-sectional bulking was low (1%–2.5%). Thus, the anti-shrink efficiency (ASE) due to the treatment was also low. The first water-submersion tests revealed some hydrophobation of the treated wood. A second submersion test, however, revealed successful hydrophobation only for betain-functionalised material. The carboxylated silicone even increased the speed of water uptake as compared to the controls. The samples treated with silicones bearing epoxy, diamino, and carboxy functionalities showed a distinct reduction in mass loss compared to the control samples after 16 weeks of incubation with the fungi Coniophora puteana and Trametes versicolor according to EN 113 and CEN/TS 15083-1, whereas the betain-functionalised silicone did not enhance fungal resistance. All silicones tested lowered the mass loss in a soft rot test according to ENv 807. The mode of action of the silicones is discussed.
Modification of Pinus sylvestris L. wood with quat- and amino-silicones of different chain lengths
Authors: Ghosh Shyamal C. , Militz Holger , Mai Carsten 
Published: November 2012

Abstract
The water-related properties of Scots pine (Pinus sylvestris L.) sapwood have been improved by treatment with quat- and amino-silicones of different chain lengths. Standard leaching test and hot water Soxhlet extraction (6 h) showed that the amino-silicones are better fixed in wood than the quat-silicones. A water dipping test (24 h) revealed that both quat- and amino-silicones made wood hydrophobic; however, amino-silicones were more effective in reducing water uptake. The long-chained silicones of both types resulted in higher water repellent effectiveness compared with the respective short-chained silicones. As demonstrated by assessing cell wall bulking, scanning electron microscopy (SEM), and SEM energy-dispersive X-ray analysis, silicones with short chains penetrated the cell wall better than those with long chains regardless of the silicone type. The maximum antishrink efficiency of approximately 60% was attained with short-chained amino-silicone at approximately 39% weight percent gain. The short-chained silicones show more potential to enhance the water-related properties of solid wood and for the development of silicone-based industrial wood modification processes.
Cloning and characterization of a 2,3-oxidosqualene cyclase from Eleutherococcus trifoliatus
Authors: Ma Li-Ting , Wang Sheng-Yang , Tseng Yen-Hsueh , Lee Yi-Ru , Chu Fang-Hua 
Published: November 2012

Abstract
The 2,3-oxidosqualene cyclases (OSCs) are a family of enzymes that have an important role in plant triterpene biosynthesis. In this study, an OSC gene designed EtLUS from Eleutherococcus trifoliatus has been cloned. EtLUS includes a 2292-bp open reading frame and encodes a 763-amino acid protein. EtLUS has an MLCYCR motif, which is conserved in lupeol synthases. Comparison of active-site residues and gene expression in yeast showed that EtLUS synthesizes lupeol. However, EtLUS has the highest sequence identity with β-amyrin synthases from Araliaceae rather than lupeol synthases, adding new perspective to the evolution of the OSCs of Araliaceae. Furthermore, EtLUS is upregulated in leaf tissues under methyl jasmonate treatment, which can be interpreted that lupeol and its derivatives play an ecological and physiological role in plant defense against pathogens and insect herbivores.
Measuring the thermal properties of green wood by the transient plane source (TPS) technique
Authors: Dupleix Anna , Kusiak Andrzej , Hughes Mark , Rossi Fréderic 
Published: November 2012

Abstract
The thermal properties of wood in the green state have been determined by the transient plane source (TPS) technique. Data are presented on thermal conductivity (λ), heat capacity (C), and thermal diffusivity (κ) at moisture contents (MCs) above the fiber saturation point, which are based on measurements using the HotDisk® apparatus. Four wood species (Douglas fir, beech, birch, and spruce) were tested, and the results are compared with literature data and those obtained by the flash method. A linear relationship was found between the thermal properties λ, C, and κ on the one hand and MC on the other. Equations predicting the thermal values as a function of MC and wood anisotropy are presented. Wood C and λ increase with MC, but wet wood diffuses heat more rapidly than dry wood.
Kinetics of Mechanical Alloying, Mechanical Properties of Micro and Nanostructural Al-C Systems
Authors: Besterci Michal , Sülleiová Katarína , Velgosová Oksana 
Published: November 2012

AbstractA method of mechanical alloying process is described. Carbon transformation to Al4C3 is characterized within the different heat treatment schedules and nine commercial carbon powders are tested. The micromechanism of carbon incorporation into the metallic powder, and its compacting are described. The influence of dispersed carbides on mechanical properties is evaluated together with the influence of deformation on microstructure and properties. It was proved that the transformation efficiency of carbon to Al4C3 by heat treatment of aluminium with the porous furnace black and electrographite is higher than that of the hard cracked graphite. Microstructure changes consisted of the fracture processes and welding of the particles with incorporation of C phase and forming of final granules. The dispersed phase Al4C3 particle size was measured on thin foil structures, and it was constant and as small as 30 nm. The particle size was influenced neither by the carbon type nor by the heat treatment technology applied. Subgrain size measured in the range of 100 grains in thin foils depended on the carbon type, as well. It ranged from 0.3 to 0.7 µm. Using a DSI (depth sensing indentation technique), the Martens hardness, indentation modulus E and deformation work W for Al matrix and Al4C3 particles have been measured. The temperature dependence of ductility, and reduction of area in the temperature range of 623–723 K and strain rate of 10−1 s−1, indicated a considerable increase of these properties. In a case when the volume fraction of Al4C3 changes from lower to higher, the grain rotation mechanism dominates instead of the grain boundary sliding. The comparison of the tensile test results and changes in fracture for the Al-Al4C3 system at two temperatures and two strain rates is summarized. The dependence of the minimum deflection rate on the applied force as well as the dependence of the time to fracture on the applied force for two temperature levels (623 and 723 K) by small punch testing are depicted. The composite was tested in two different states: a) as received by mechanical alloying with hot extrusion (HE) as the final operation and b) ECAPed (mean grain size of 100–200 nm). The dependence of the minimum deflection rate on the applied force as well as the dependence of the time to fracture on the applied force for two temperature levels are evaluated. The anisotropy of the creep properties and fracture using small punch tests for the Al-Al4C3 system produced by ECAP were analysed.
Educating for the future: adolescent girls’ health and education in West Bengal, India
Authors: Rees Chris A. , Long Katelyn N. , Gray Bobbi , West Joshua H. , Chanani Sheila , Spielberg Freya , Crookston Benjamin T. 
Published: November 2012

Abstract
Adolescent girls in India carry a disproportionate burden of health and social risks; girls that do not finish secondary education are more likely to have an earlier age of sexual initiation, engage in risky sexual behavior, and consequentially be at greater risk of dying from pregnancy-related causes. This paper presents a comparison of girls in school and girls not in school from 665 participants in rural West Bengal, India. The social cognitive theory (SCT), a comprehensive theoretical model, was used as a framework to describe the personal, behavioral, and environmental factors affecting the lives of these adolescent girls. There were significant differences between girls in and out of school in all three categories of the SCT; girls in school were more likely to have heard of sexually transmitted diseases or infections than girls not in school (p<0.0001). Girls in school were also more likely than girls not in school to boil water before drinking (p=0.0078), and girls in school lived in dwellings with 2.3 rooms on average, whereas girls not in school lived in dwellings with only 1.7 rooms (p<0.0001). Indian adolescent girls who are not in school are disadvantaged both economically and by their lack of health knowledge and proper health behaviors when compared with girls who are still in school. In addition, to programs to keep girls in school, efforts should also be made to provide informal education to girls not in school to improve their health knowledge and behaviors.
Internet use, misuse, and addiction in adolescents: current issues and challenges
Authors: Greydanus Donald E. , Greydanus Megan M. 
Published: November 2012

Abstract
The Internet has revolutionized education and social communication in the 21st century. This article reviews the growing literature identifying a number of adolescents and young adults with a pathologically excessive Internet use leading to many potential consequences. Current research dilemmas in this area include that Internet addiction is a broad topic with no standard definition and no standard measurement tools. Management of youth with identified problematic Internet use or misuse centers on behavioral therapy and treatment of comorbidities. Pharmacologic approaches are limited at this time but are undergoing research, such as use of opioid antagonists and antidepressants in adults with pathological gambling. Efforts should be expanded on not only the education of all adolescents regarding the benefits but also the potential negative consequences of Internet use. It is vital that we do this for Generation Z, whereas Generation ALPHA will soon benefit or suffer from our efforts in this regard today.
Early executive function deficit in preterm children and its association with neurodevelopmental disorders in childhood: a literature review
Authors: Sun Jing , Buys Nicholas 
Published: November 2012

Abstract
The purpose of this study is to examine the association of deficits of executive function (EF) and neurodevelopmental disorders in preterm children and the potential of assessing EF in infants as means of early identification. EF refers to a collection of related but somewhat discrete abilities, the main ones being working memory, inhibition, and planning. There is a general consensus that EF governs goal-directed behavior that requires holding those plans or programs on-line until executed, inhibiting irrelevant action and planning a sequence of actions. EF plays an essential role in cognitive development and is vital to individual social and intellectual success. Most researchers believe in the coordination and integrate cognitive–perceptual processes in relation to time and space, thus regulating higher-order cognitive processes, such as problem solving, reasoning, logical and flexible thinking, and decision-making. The importance of the maturation of the frontal lobe, particularly the prefrontal cortex, to the development of EF in childhood has been emphasized. Therefore, any abnormal development in the prefrontal lobes of infants and children could be expected to result in significant deficits in cognitive functioning. As this is a late-maturing part of the brain, various neurodevelopmental disorders, such as autism spectrum disorders, attention deficit hyperactivity disorder, language disorders, and schizophrenia, as well as acquired disorders of the right brain (and traumatic brain injury) impair EF, and the prefrontal cortex may be particularly susceptible to delayed development in these populations. The deficits of EF in infants are persistent into childhood and related to neurodevelopmental disorders in childhood and adolescence.
Access to contraception and HIV testing among young women in a peri-urban district of Uganda
Authors: Mbonye Anthony K. , Wamono Felix 
Published: November 2012

Abstract
Objective: The purpose of the study was to assess care-seeking practices for contraception and human immune deficiency virus (HIV) testing services among young women (14–24 years) in a peri-urban district of Uganda.
Methods: A formative study assessed care-seeking practices among women in Wakiso district, Central Uganda. A survey was carried out in 11 randomly selected parish clusters targeting women in the reproductive age group. Data on current use of contraception, information from women who had been tested for HIV, future desire for an HIV test, and choice of care were captured.
Results: A total of 10,993 women were interviewed of whom young women were 5384 (49.0%). The proportion of young women using a method of contraception was less, 2446 (45.8%), compared with 2832 (54.3%) for older women, p=0.0001. Few adolescents (14–19 years), 850 (45.3%), had tested for HIV compared with older women, p=0.0001, whereas majority of the young women, 1321 (75.5%), wished to have an HIV test compared with older women, 742 (66.0%), p=0.0001. Fear for an HIV test was common among women of both age groups, p=0.9. Data further show that young women were less likely to deliver at hospitals, 1582 (50.8%), compared with older women, 2586 (53.3%), p=0.0001. Similarly, a lesser proportion of young women, 845 (50.4%), sought contraception at hospitals compared with older women, 1432 (54.4%), p=0.004. The factors that most influenced access to services were age, educational level, source of care, marital status, and future intention to use a particular service.
Conclusions: Young women are less likely to access contraception and HIV testing services in this district. These results will be useful in designing and evaluating future interventions targeting young women.
Exploring resilience: strengths of trafficking survivors in Cambodia
Authors: Gray Glori G. , Luna Lilia , Seegobin Winston 
Published: November 2012

Abstract
Western literature provides an array of information regarding resilience within at-risk youth. Resilience research within non-Western contexts, and more specifically with exploited youth, is more limited. Despite exploitation, some youth develop a hardy ability to overcome adversity, allowing them more mastery over their environments and even increased psychological steadfastness. This project involved exploring the protective factors of resilience and psychological functioning in Cambodian youth, specifically a group of 24 survivors of sexual trafficking and another group of 24 rural youth without reported exploitation. The ages of participants ranged from 13 to 22 years, with the average age being 15.62 years [standard deviation (SD=2.68)]. Results indicated resilience constructs (mastery and relatedness) correlated with psychological functioning (anxiety and depression), as expected. The sense of relatedness was moderately associated with age. Also, as predicted, the trafficked young women demonstrated more resilience and less pathology. Consistent with previous research, earlier trauma is believed to inoculate survivors of trauma against further stress, mobilize them to better confront adversity and reduce psychological disruptions. Understanding these issues can help in understanding the relationship between resilience factors and psychological functioning as well as the strengths of many trauma survivors. Their strengths are particularly useful for developing effective treatment protocols for traumatized youth from non-Western backgrounds.
Deliberate self-poisoning: a study from Nablus
Authors: Sawalha Ansam F. 
Published: November 2012

Abstract
Aim: Arab Palestinians have experienced intense political, domestic and economic stressful conditions, which can increase the risk of self-harm. No previous studies haves been published to describe the patterns and rates of deliberate self-harm, particularly self-poisoning, in the Palestinian Authority. The objective of this study was to investigate deliberate self-poisoning in the Palestinian Authority.
Methods: All patients admitted to the emergency department of Al-Watani Governmental Hospital from May 2008 to April 2009 and identified as deliberate self-poisoning were investigated. The demographic and clinical data of the subjects were analyzed using SPSS16.
Results: A total of 54 individuals were identified. The mean age of these patients was 23.8±7.9 years and less than one-third (16; 29.6%) were under 18 years of age. The majority of the patients were female (35; 64.8%). Two-thirds (37; 68.5%) of the patients were Nablus city residents. An overdose of pharmaceutical products (39; 72.2%) was the most common method of self-poisoning. Significant associations were found between the type of material used in self-poisoning and place of residence (p=0.008) as well as gender (p=0.021). Gastric lavage was performed for 23 (42.6%) individuals, while only four (7.4%) received activated charcoal, and six (11.1%) had both.
Conclusion: Rates of deliberate self-poisoning in the Palestinian Authority are lower than those reported from developed countries, but higher than that reported from other Arab countries. Regulations regarding the sale and storage of poisonous agricultural chemicals are needed in the Palestinian Authority.
Can prenatal detection of Down syndrome be improved by enhancing obstetricians’ skills of performing adequate foetal cardiac examination at the primary level?a
Authors: Gersak Ksenija , Vesel Samo 
Published: November 2012

Abstract

Aims: To evaluate the prevalence of congenital heart defects (CHDs) in live-born infants with Down syndrome (DS) and to investigate whether these CHDs might be detected during routine second trimester ultrasound screening performed at the primary level.

Methods: A retrospective analysis of 66 cases of DS in live-born infants. The infants with DS underwent a detailed echocardiographic examination to evaluate cardiac morphological characteristics and function.

Results: Thirty-six live-born DS infants (54.5%) had associated CHDs. According to the apical four-chamber view at the first postnatal echocardiographic examination, we estimated that 20 (55.6%) of the 36 patients with associated CHDs should have been identified during the routine second-trimester prenatal scan [17 infants with complete atrioventricular septal defect (AVSD), two with partial AVSD, and one with non-restrictive perimembranous ventricular septal defect] if the results had been correctly interpreted. An additional seven patients with associated CHDs should have been identified if the evaluation of both outflow tracts had been included into the screening protocol.

Conclusion: Our data suggest that the prenatal DS detection rate can be significantly increased by improving obstetricians’ skills of performing adequate foetal cardiac examination as part of the routine 18- to 23-week ultrasound examination at the primary level.