bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequence of Brucella melitensis Biovar 3 Strain NI, Isolated from an Aborted Bovine Fetus
Authors: Liu Wenxiao , Jing Zhigang , Ou Qixing , Cui Buyun , He Yongqun , Wu Qingmin 
Published: October 2012

ABSTRACT

From an aborted bovine fetus in China, a bacterial strain named NI was isolated and identified as
Brucella melitensis
by a PCR assay. Strain NI was further characterized as
B. melitensis
biovar 3 using biochemical assays. Here we report the complete genome sequence of strain NI.
Complete Genome Sequence of Bartonella quintana, a Bacterium Isolated from Rhesus Macaques
Authors: Li Hao , Tong Yigang , Huang Yong , Bai Jieying , Yang Hong , Liu Wei , Cao Wuchun 
Published: October 2012

ABSTRACT

Bartonella quintana
is a re-emerging pathogen and the causative agent of a broad spectrum of disease manifestations in humans. The present study reports the complete genome of
B. quintana
strain RM_11, which was isolated from rhesus macaques.
Draft Genome Sequence of Alicyclobacillus hesperidum Strain URH17-3-68
Authors: Wang Ping , Li Li , Chen Xiaoyun , Jiang Nan , Liu Guiyou , Chen Lawrence , Xu Jianyong , Song Hui , Chen Zugen , Ma Yanhe 
Published: October 2012

ABSTRACT

Alicyclobacillus hesperidum
is a thermoacidophilic bacterium. We isolated strain URH17-3-68 from hot spring sludge in Tengchong, Yunnan province, China. Its extracellular products include heat- and acid-stable enzymes which are important for industrial applications. Here we report the draft genome of this strain.
Draft Genome Sequence of the Xylan-Degrading Marine Bacterium Strain S124, Representing a Novel Species of the Genus Oceanicola
Authors: Kwon Young-Kyung , Kim Jennifer Jooyoun , Kim Ji Hyung , Jeon Seon-Mi , Ye Bo-Ram , Jang Jiyi , Heo Soo-Jin , Park Se Chang , Kang Do-Hyung , Oh Chulhong 
Published: October 2012

ABSTRACT

We isolated a xylan-degrading bacterium from seawater of Micronesia and identified it as
Oceanicola
sp. strain S124. We sequenced the
Oceanicola
sp. S124 genome using GSFLX 454 pyrosequencing and predicted 4,433 open reading frames (ORFs) including putative saccharification and phage-related genes.
Draft Genome Sequence of the Sulfobacillus thermosulfidooxidans Cutipay Strain, an Indigenous Bacterium Isolated from a Naturally Extreme Mining Environment in Northern Chile
Authors: Travisany Dante , Di Genova Alex , Sepúlveda Andrea , Bobadilla-Fazzini Roberto A. , Parada Pilar , Maass Alejandro 
Published: October 2012

ABSTRACT

Sulfobacillus thermosulfidooxidans
strain Cutipay is a mixotrophic, acidophilic, moderately thermophilic bacterium isolated from mining environments of the north of Chile, making it an interesting subject for studying the bioleaching of copper. We introduce the draft genome sequence and annotation of this strain, which provide insights into its mechanisms for heavy metal resistance.
Complete Genome Sequence of Lactobacillus helveticus R0052, a Commercial Probiotic Strain
Authors: Tompkins Thomas A. , Barreau Guillaume , Broadbent Jeffery R. 
Published: October 2012

ABSTRACT

Lactobacillus helveticus
R0052 is a commercially available strain that is widely used in probiotic preparations. The genome sequence consisted of 2,129,425 bases. Comparative analysis showed that it was unique among
L. helveticus
strains in that it contained genes encoding mucus-binding proteins similar to those found in
Lactobacillus acidophilus
.
Draft Genome Sequence of Bacillus oceanisediminis 2691
Authors: Lee Yong-Jik , Lee Sang-Jae , Jeong Haeyoung , Kim Hyun Ju , Ryu Naeun , Kim Byoung-Chan , Lee Han-Seung , Lee Dong-Woo , Lee Sang Jun 
Published: October 2012

ABSTRACT

Bacillus oceanisediminis
2691 is an aerobic, Gram-positive, spore-forming, and moderately halophilic bacterium that was isolated from marine sediment of the Yellow Sea coast of South Korea. Here, we report the draft genome sequence of
B. oceanisediminis
2691 that may have an important role in the bioremediation of marine sediment.
Draft Genome Sequence of Achromobacter piechaudii Strain HLE
Authors: Trimble William L. , Phung Le T. , Meyer Folker , Silver Simon , Gilbert Jack A. 
Published: October 2012

ABSTRACT

Achromobacter piechaudii
strain HLE is a betaproteobacterium (previously known as
Alcaligenes faecalis
) that was an early isolate with arsenite oxidase activity. This draft genome of 6.89 Mb is the second available genome for this species in the opportunistic pathogen
Alcaligenaceae
family.
Draft Genome Sequence of Bacillus anthracis BF-1, Isolated from Bavarian Cattle
Authors: Antwerpen Markus , Proença Diogo Neves , Rückert Christian , Licht Katharina , Kalinowski Jörn , Hanczaruk Matthias , Tiemann Carsten , Grass Gregor 
Published: October 2012

ABSTRACT

Bacillus anthracis
BF-1 was isolated from a cow in Bavaria (Germany) that had succumbed to anthrax. Here, we report the draft genome sequence of this strain, which belongs to the European B2 subclade of
B. anthracis
. The closest phylogenetic neighbor of strain BF-1 is a strain isolated from cattle in France.
The Effectiveness of Acupuncture for Plantar Heel Pain: A Systematic Review
Authors: Clark Richard James , Tighe Maria 
Published: October 2012

Introduction Plantar heel pain (PHP) is a common complaint, yet there are no definitive guidelines for its treatment. Acupuncture is increasingly used by podiatrists, and there is a need for evidence to validate this practice. It is acknowledged that PHP and acupuncture are both complex phenomena. Method A systematic review (PROSPERO no. CRD42012001881) of the effectiveness of acupuncture for PHP is presented. Quality of the studies was assessed by independent assessors with reference to Quality Index (QI), ‘STandards for Reporting Interventions in Controlled Trials of Acupuncture’ (STRICTA) and ‘CONsolidated Standards Of Reporting Trials’ (CONSORT) criteria. Pooling of data, or even close comparison of studies, was not performed. Results Five randomised controlled trials and three non-randomised comparative studies were included. High quality studies report significant benefits. In one, acupuncture was associated with significant improvement in pain and function when combined with standard treatment (including non-steroidal anti-inflammatory drugs). In another, acupuncture point PC7 improved pain and pressure pain threshold significantly more than LI4. Other papers were of lower quality but suggest benefits from other acupuncture approaches. Conclusions There is evidence supporting the effectiveness of acupuncture for PHP. This is comparable to the evidence available for conventionally used interventions, such as stretching, night splints or dexamethasone. Therefore acupuncture should be considered in recommendations for the management of patients with PHP. Future research should recognise the complexity of PHP, of acupuncture and of the relationship between them, to explore the optimum use and integration of this approach. There is a need for more uniformity in carrying out and reporting such work and the use of STRICTA is recommended.
Translation and testing of the Risk Assessment Pressure Ulcer Sore scale used among residents in Norwegian nursing homes
Authors: Fossum Mariann , Söderhamn Olle , Cliffordson Christina , Söderhamn Ulrika 
Published: October 2012

ObjectiveThe purpose of this study was to translate and test the psychometric properties of the Norwegian-language version of the Risk Assessment Pressure Sore (RAPS) scale.BackgroundRisk assessment scales for pressure ulcer (PU) prevention have become an aspect of quality improvement in healthcare, but their effectiveness depends on the reliability and validity of the scale.Methods A convenience sample of 481 residents in 15 nursing homes in rural Norway was included between January and June 2007. The English-language version of the RAPS scale was translated into Norwegian, and this scale was used to collect the data, including a skin examination. The number of PUs and grades were documented. Reliability was assessed in a small group of 26 residents and construct validity in the total study group.ResultsEquivalence between two assessments regarding total scores of the RAPS scale was reflected in an intraclass correlation coefficient (ICC) of 0.95. Construct validity was supported, and the RAPS scale could define groups with expected low and high scores. Further evidence of construct validity was shown in a confirmatory factor analysis.ConclusionThe Norwegian version of the RAPS scale has shown sufficient psychometric properties to be considered a reliable and valid scale for identifying risk of PUs among nursing home residents. However, further testing is needed.
Consensus diagnostic criteria for fetal alcohol spectrum disorders in Australia: a modified Delphi study
Authors: Watkins Rochelle E , Elliott Elizabeth J , Mutch Raewyn C , Payne Janet M , Jones Heather M , Latimer Jane , Russell Elizabeth , Fitzpatrick James P , Hayes Lorian , Burns Lucinda , Halliday Jane , D'Antoine Heather A , Wilkins Amanda , Peadon Elizabeth , Miers Sue , Carter Maureen , O'Leary Colleen M , McKenzie Anne , Bower Carol 
Published: October 2012

ObjectiveTo evaluate health professionals' agreement with components of published diagnostic criteria for fetal alcohol spectrum disorders (FASD) in order to guide the development of standard diagnostic guidelines for Australia.DesignA modified Delphi process was used to assess agreement among health professionals with expertise or experience in FASD screening or diagnosis. An online survey, which included 36 Likert statements on diagnostic methods, was administered over two survey rounds. For fetal alcohol syndrome (FAS), health professionals were presented with concepts from the Institute of Medicine (IOM), University of Washington (UW), Centers for Disease Control (CDC), revised IOM and Canadian diagnostic criteria. For partial FAS (PFAS), alcohol-related neurodevelopmental disorder (ARND), and alcohol-related birth defects (ARBD), concepts based on the IOM and the Canadian diagnostic criteria were compared.Setting/participants130 Australian and 9 international health professionals.ResultsOf 139 health professionals invited to complete the survey, 103 (74.1%) responded, and 74 (53.2%) completed one or more questions on diagnostic criteria. We found consensus agreement among participants on the diagnostic criteria for FAS, with the UW criteria most commonly endorsed when compared with all other published criteria for FAS. When health professionals were presented with concepts based on the Canadian and IOM diagnostic criteria, we found consensus agreement but no clear preference for either the Canadian or IOM criteria for the diagnosis of PFAS, and no consensus agreement on diagnostic criteria for ARND. We also found no consensus on the IOM diagnostic criteria for ARBD.ConclusionsParticipants indicated clear support for use of the UW diagnostic criteria for FAS in Australia. These findings should be used to develop guidelines to facilitate improved awareness of, and address identified gaps in the infrastructure for, FASD diagnosis in Australia.
The collaborative communication model for patient handover at the interface between high-acuity and low-acuity care
Authors: Toccafondi Giulio , Albolino Sara , Tartaglia Riccardo , Guidi Stefano , Molisso Antonio , Venneri Francesco , Peris Adriano , Pieralli Filippo , Magnelli Elisabetta , Librenti Marco , Morelli Marco , Barach Paul 
Published: October 2012

BackgroundCross-unit handovers transfer responsibility for the patient among healthcare teams in different clinical units, with missed information, potentially placing patients at risk for adverse events.ObjectivesWe analysed the communications between high-acuity and low-acuity units, their content and social context, and we explored whether common conceptual ground reduced potential threats to patient safety posed by current handover practices.MethodsWe monitored the communication of five content items using handover probes for 22 patient transitions of care between high-acuity ‘sender units’ and low-acuity ‘recipient units’. Data were analysed and discussed in focus groups with healthcare professionals to acquire insights into the characteristics of the common conceptual ground.ResultsHigh-acuity and low-acuity units agreed about the presence of alert signs in the discharge form in 40% of the cases. The focus groups identified prehandover practices, particularly for anticipatory guidance that relied extensively on verbal phone interactions that commonly did not involve all members of the healthcare team, particularly nursing. Accessibility of information in the medical records reported by the recipient units was significantly lower than reported by sender units. Common ground to enable interpretation of the complete handover content items existed only among selected members of the healthcare team.ConclusionsThe limited common ground reduced the likelihood of correct interpretation of important handover information, which may contribute to adverse events. Collaborative design and use of a shared set of handover content items may assist in creating common ground to enable clinical teams to communicate effectively to help increase the reliability and safety of cross-unit handovers.
Medication discrepancies in integrated electronic health records
Authors: Linsky Amy , Simon Steven R 
Published: October 2012

IntroductionMedication discrepancies are associated with adverse drug events. Electronic health records (EHRs) may reduce discrepancies, especially if integrated with pharmacy dispensing. We determined the prevalence of discrepancies within a national healthcare system with EHR–pharmacy linkage to characterise the medications involved and to identify factors associated with discrepancies.MethodsWe conducted a retrospective cohort study of ambulatory care patients at Veterans Affairs Boston Healthcare System, April 2010–July 2011. The primary outcome was the presence of any medication discrepancy or specific types of discrepancies: commission—present in the record but not taken by patient; omission—not present in the record; duplication—present more than once; or alteration in dose or frequency—present but taken differently than documented.ResultsSixty-two patients (60%) had at least one medication discrepancy. Prevalence of commissions, omissions, duplications and alterations were 36%, 27%, 11% and 19%, respectively. The involved medications differed by type of discrepancy, but non-opioid analgesics and herbal therapies were common among commissions and omissions. In adjusted analyses, an increasing number of medications was associated with more commissions (OR 1.2; 95% CI 1.1 to 1.3) and duplications (OR 1.2; 95% CI 1.1 to 1.4) and fewer omissions (OR 0.9; 95% CI 0.8 to 1.0).DiscussionIn a system with a well established EHR linked to pharmacy dispensing, medication discrepancies occurred in 60% of ambulatory clinic patients. Patients with a greater number of medications were more likely to have errors of commission and duplication, but less likely to have errors of omission. Our findings highlight that relying on EHRs alone will not ensure an accurate medication list and stress the need to review medication taking thoroughly with patients to capitalise on the full potential of EHRs.
The effectiveness of a specially designed shoulder chair for closed reduction of acute shoulder dislocation in the emergency department: a randomised control trial
Authors: Chung Josephine Yuen Man , Cheng Chi Hung , Graham Colin A , Rainer Timothy H 
Published: October 2012

Study objectiveThe objective of this study was to demonstrate the effectiveness of a specially designed chair for closed reduction of acute shoulder dislocations.MethodThis was a prospective, non-blinded randomised controlled trial conducted in a university affiliated emergency department (ED). The inclusion criteria were (1) age ≥18 years; (2) anterior or posterior shoulder dislocation without fracture of the surgical neck of the humerus; (3) patient who is able to communicate and cooperate. Participants were randomly assigned using a computer generated random number sequence into one of two groups—either the traditional practice group or Oxford chair group. Administration of intravenous sedation was only permitted in the traditional practice group due to the concerns of sedation use in the sitting position while unsupported on the chair. The primary outcome measure was length of ED stay. The secondary outcome measures were length of time for the procedure, successful reduction rate, levels of pain experienced by patients in different time periods before and after the reduction.ResultSixty eligible patients were recruited, 30 in each group. The median lengths of stay in the ED in Oxford chair group (n=30) and traditional method group (n=30) were152 min and 173 min respectively (p=0.183). The median procedure time was 3 min for the Oxford chair group compared to 5 min in the traditional method group (p=0.179). The success rate for the Oxford chair method was 77% (23/30). There were no statistically or clinically significant differences of pain score at any point.ConclusionsThe chair method had a 77% success rate in reducing acute shoulder dislocations without sedation. There was no difference in pain level experienced by patients between the chair method and the traditional method. Patient factors, including patients who have had previous shoulder surgery and patients who have fracture dislocations, contribute to the reduced efficacy of the chair method. It remains possible that the chair method may reduce patient length of stay in the ED in uncomplicated patients.
Sexual activity-related emergency department admissions: eleven years of experience at a Swiss university hospital
Authors: Pfortmueller Carmen Andrea , Koetter Jana Nimia , Zimmermann Heinz , Exadaktylos Aristomenis Konstantinos 
Published: October 2012

PrincipalsMost people enjoy sexual intercourse without complications, but a significant, if small, number need to seek emergency medical help for related health problems. The true incidence of these problems is not known. We therefore assessed all admissions to our emergency department (ED) in direct relation to sexual intercourse.MethodsAll data were collected prospectively and entered into the ED's centralised electronic patient record database (Qualicare, Switzerland) and retrospectively analysed. The database was scanned for the standardised key words: ‘sexual intercourse’ (German ‘Geschlechtsverkehr’) or ‘coitus’ (German ‘Koitus’).ResultsA total of 445 patients were available for further evaluation; 308 (69.0%) were male, 137 (31.0%) were female. The median age was 32 years (range 16–71) for male subjects and 30 years (range 16–70) for female subjects. Two men had cardiovascular emergencies. 46 (10.3%) of our patients suffered from trauma. Neurological emergencies occurred in 55 (12.4%) patients: the most frequent were headaches in 27 (49.0%), followed by subarachnoid haemorrhage (12, 22.0%) and transient global amnesia (11, 20.0%). 154 (97.0%) of the patients presenting with presumed infection actually had infections of the urogenital tract. The most common infection was urethritis (64, 41.0%), followed by cystitis (21, 13.0%) and epididymitis (19, 12.0%). A sexually transmitted disease (STD) was diagnosed in 43 (16.0%) of all patients presenting with a presumed infection. 118 (43.0%) of the patients with a possible infection requested testing for an STD because of unsafe sexual activity without underlying symptoms.ConclusionsSexual activity is mechanically dangerous, potentially infectious and stressful for the cardiovascular system. Because information on ED presentation related to sexual intercourse is scarce, more efforts should be undertaken to document all such complications to improve treatment and preventative strategies.
Effective Treatment of Edema and Endothelial Barrier Dysfunction With Imatinib
Authors: Aman Jurjan , van Bezu Jan , Damanafshan Amin , Huveneers Stephan , Eringa Etto C. , Vogel Steven M. , Groeneveld A.B. Johan , Vonk Noordegraaf Anton , van Hinsbergh Victor W.M. , van Nieuw Amerongen Geerten P. 
Published: October 2012

Background—
Tissue edema and endothelial barrier dysfunction as observed in sepsis and acute lung injury carry high morbidity and mortality, but currently lack specific therapy. In a recent case report, we described fast resolution of pulmonary edema on treatment with the tyrosine kinase inhibitor imatinib through an unknown mechanism. Here, we explored the effect of imatinib on endothelial barrier dysfunction and edema formation.

Methods and Results—
We evaluated the effect of imatinib on endothelial barrier function in vitro and in vivo. In human macro- and microvascular endothelial monolayers, imatinib attenuated endothelial barrier dysfunction induced by thrombin and histamine. Small interfering RNA knock-downs of the imatinib-sensitive kinases revealed that imatinib attenuates endothelial barrier dysfunction via inhibition of Abl-related gene kinase (Arg/Abl2), a previously unknown mediator of endothelial barrier dysfunction. Indeed, Arg was activated by endothelial stimulation with thrombin, histamine, and vascular endothelial growth factor. Imatinib limited Arg-mediated endothelial barrier dysfunction by enhancing Rac1 activity and enforcing adhesion of endothelial cells to the extracellular matrix. Using mouse models of vascular leakage as proof-of-concept, we found that pretreatment with imatinib protected against vascular endothelial growth factor–induced vascular leakage in the skin, and effectively prevented edema formation in the lungs. In a murine model of sepsis, imatinib treatment (6 hours and 18 hours after induction of sepsis) attenuated vascular leakage in the kidneys and the lungs (24 hours after induction of sepsis).

Conclusions—
Thus, imatinib prevents endothelial barrier dysfunction and edema formation via inhibition of Arg. These findings identify imatinib as a promising approach to permeability edema and indicate Arg as novel target for edema treatment.
Barriers to the uptake of eye care services in developing countries: A systematic review of interventions
Authors: Abdullah Khadija Nowaira , Al-Sharqi Omar Zayan , Abdullah Muhammad Tanweer 
Published: October 2012

Objective: This research identifies effective and ineffective interventions for reducing barriers to the uptake of eye care services in developing countries. Design: Systematic literature review. Setting: Only research studies done in developing countries were included. Method: The review is restricted to English-language articles published between 1997 and 2007. It includes studies that reported randomized trials (controlled and uncontrolled) and surveys with some form of outcome measure, with or without process evaluation. The literature search was conducted on six electronic databases. Data were extracted and synthesized using a standard data extraction form. A narrative synthesis was carried out for the purpose of this review. The quality of the included trials was assessed by CRD guidelines and Crombie’s checklist. The applicability of research findings was evaluated by the RE-AIM model. Results: One randomized cluster trial and nine surveys were critically appraised. Three multi-faceted interventions were judged effective while another three were found ineffective. Two out of the three effective interventions used service provision and educational input. Two out of the three ineffective interventions also used service provision alongside other methods including screening and counselling. Findings of effective interventions were generalizable. Conclusion: The review suggests that eye health education and service provision lessen the barriers to service uptake and increase the uptake of eye care services. The role of counselling and screening services needs further review by large and good-quality studies.