bluuebunny/crossref_metadata_2025_split huggingface dataset:
Suppression of tongue squamous cell carcinoma growth by inhibition of Jagged1 in vitro and in vivo
Authors: Zhang Tong‐han , Liu Hai‐chao , Liang Yu‐jie , Liang Li‐zhong , Zheng Guang‐sen , Huang Hong‐zhang , Wu Ji‐nan , Liao Gui‐qing 
Published: November 2012

BackgroundThe changes in Notch signaling are closely related to the occurrence and development of many cancers. We have investigated Notch signaling receptor and its ligand expressions in TSCC cell lines, tissues and its significance. We clarified Notch signaling pathway in TSCC and its mechanism. We regulated Notch signaling pathway of tumor cells, thereby inhibiting tumor cell proliferation and differentiation.MethodsWe detected Jagged1 protein and mRNA expression levels in specimens (tongue cancer and adjacent tissues) from 74 patients with tongue cancer and in TSCC cell line. The Jagged1‐targeted lentiviral vector RNAi system was constructed, and its suppressive effects on the proliferation and invasion of tongue carcinoma cells in in vivo and ex vivo were determined.ResultsJagged1 was expressed in tongue squamous cell cancer tissues and cell line, but there were differences in its expression. Jagged1 was knocked down and the tumor growth was inhibited accompanying cell cycle changes. Animal studies also showed that the tumor growth was inhibited.ConclusionsJagged1 may be involved in the differentiation and proliferation of tongue cancer. Targeting Jagged1 RNA interference lentiviral vector can effectively lower Jagged1 mRNA and protein expression levels of Tca8113 cells, thereby preventing the proliferation of TSCC cells. Jagged1 is expected to be a promising new target for curing tongue cancer. In‐depth study of the interaction between Jagged1 and other molecules of Notch signaling pathway in the process of carcinogenesis has important theoretical guidance and clinical significance in revealing the mechanism of Jagged1 and its application in the therapy for tongue cancer.
Foreign Direct Investment, Trade, and Skilled Labour Demand in Eastern Europe
Authors: Bruno Giovanni S. F. , Crinò Rosario , Falzoni Anna M. 
Published: November 2012

Abstract. In this paper, we study the effects of inward foreign direct investment (FDI) and trade on relative skilled labour demand in Poland, Hungary, and the Czech Republic. Our estimates show significant heterogeneity in the FDI effect across the three economies: the effect is always significantly positive for Hungary, weakly negative for Poland, and negligible for the Czech Republic. As to trade, we find much more homogeneity in coefficient estimates, which are generally negative for all countries, although significant only in the case of Hungary.
Evaluating the ability of Bayesian clustering methods to detect hybridization and introgression using an empirical red wolf data set
Authors: Bohling Justin H. , Adams Jennifer R. , Waits Lisette P. 
Published: November 2012

AbstractBayesian clustering methods have emerged as a popular tool for assessing hybridization using genetic markers. Simulation studies have shown these methods perform well under certain conditions; however, these methods have not been evaluated using empirical data sets with individuals of known ancestry. We evaluated the performance of two clustering programs,bapsandstructure, with genetic data from a reintroduced red wolf (Canis rufus) population in North Carolina, USA. Red wolves hybridize with coyotes (C. latrans), and a single hybridization event resulted in introgression of coyote genes into the red wolf population. A detailed pedigree has been reconstructed for the wild red wolf population that includes individuals of 50–100% red wolf ancestry, providing an ideal case study for evaluating the ability of these methods to estimate admixture. Using 17 microsatellite loci, we tested the programs using different training set compositions and varying numbers of loci.structurewas more likely thanbapsto detect an admixed genotype and correctly estimate an individual's true ancestry composition. However,structurewas more likely to misclassify a pure individual as a hybrid. Both programs were outperformed by a maximum‐likelihood‐based test designed specifically for this system, which never misclassified a hybrid (50–75% red wolf) as a red wolf or vice versa. Training set composition and the number of loci both had an impact on accuracy but their relative importance varied depending on the program. Our findings demonstrate the importance of evaluating methods used for detecting admixture in the context of endangered species management.
Predictors of neurological outcome in cooled neonates
Authors: Li Jingang , Funato Masahisa , Tamai Hiroshi , Wada Hiroshi , Nishihara Masato , Iwamoto Hirotaka , Okazaki Yoko , Shintaku Haruo 
Published: November 2012

AbstractBackgroundWe define clinical predictors of neurological outcome in neonates with hypoxic–ischemic encephalopathy undergoing hypothermia therapy.MethodsTwenty‐one neonates who underwent selective head cooling between 2004 and 2010 and were followed neurologically for ≥24 months were investigated retrospectively. Patients were divided according to the neurological outcome at 2 years of age into group A (n = 11), patients with normal neurological function, and group B (n = 10), patients with neurological disabilities (n = 9) or those who died (n = 1). Predictors were determined by χ2 and Mann–Whitney U‐tests, anova, Spearman rank correlations and receiver–operator curves.ResultsGroup B showed higher average blood lactate levels during the first day, particularly at 24 h of life; lower day‐3 cerebral blood flow resistance index; higher maximum dobutamine dose used; higher rate of thiamylal sodium used; more severe background electroencephalogram suppression during the first week (group A: 11/11 cases ≤ grade 3; group B: 7/9 cases at grade 4–5) and higher rate of cerebral lesions on magnetic resonance imaging in the second week (group A: 1/11 case; group B: 9/10 cases) than group A. The most useful predictor of poor prognosis was cerebral parenchymal lesions on magnetic resonance imaging with 90%, 90% and 90% of sensitivity, specificity and accuracy, followed by week‐1 background electroencephalogram ≥ grade 4 with 70%, 100% and 85% and day‐3 cerebral blood flow resistance index < 0.46 with 71%, 88% and 80%, respectively.ConclusionsPrediction of post‐cooling neurological outcome could be improved substantially by evaluating multiple factors.
Genome Sequence of Canine Parvovirus Strain SC02/2011, Isolated from a Puppy with Severe Diarrhea in South China
Authors: Ju Chunmei , Cheng Yi , Ji Yikuan , Wang Yu , Sun Leilei , Huang Jiaxin 
Published: November 2012

ABSTRACT
A widespread hemorrhagic gastroenteritis in young dogs occurred in South China. A virulent field canine parvovirus (CPV) strain, SC02/2011, was isolated from a puppy showing enteric signs in Guangdong, China. The genome of CPV strain SC02/2011 was sequenced and analyzed, which will promote a better understanding of the molecular epidemiology and genetic diversity of CPV field isolates in South China.
Complete Genome Sequence of a Mink Calicivirus in China
Authors: Yang Bochao , Wang Fengxue , Zhang Shuqin , Xu Guicai , Wen Yongjun , Li Jianxi , Yang Zhiqiang , Wu Hua 
Published: November 2012

ABSTRACT
We report the complete genome sequence of a novel calicivirus isolated from a diseased mink in China. The complete viral genome is approximately 8.4 kb in length and consists of three open reading frames. The availability of the complete genome sequence is helpful for further investigation into the molecular characteristics and epidemiology of calicivirus in mink.
Complete Genome Sequence of an Amur Virus Isolated from Apodemus peninsulae in Northeastern China
Authors: Yao Li-Si , Zhao Hui , Shao Li-Jun , Liu Yong-Xian , Zhang Xiao-Long , Wang Jing , Deng Yong-Qiang , Li Xiao-Feng , Hu Kong-Xin , Qin Cheng-Feng , Xu Bao-Liang 
Published: November 2012

ABSTRACT

Amur virus was recently identified as the causative agent of hemorrhagic fever with renal syndrome. Here we report the complete genome sequence of an Amur virus isolated from
Apodemus peninsulae
in Northeastern China. The sequence information provided here is critical for the molecular epidemiology and evolution of Amur virus in China.
Complete Genome Sequence of the English Isolate of Rat Cytomegalovirus (
Murid Herpesvirus 8
)
Authors: Ettinger Jakob , Geyer Henriette , Nitsche Andreas , Zimmermann Albert , Brune Wolfram , Sandford Gordon R. , Hayward Gary S. , Voigt Sebastian 
Published: November 2012

ABSTRACT

The complete genome of the English isolate of rat cytomegalovirus (RCMV-E) was determined. RCMV-E has a 202,946-bp genome with noninverting repeats but without terminal repeats. Thus, it differs significantly in size and genomic arrangement from closely related rodent cytomegaloviruses (CMVs). To account for the differences between the rat CMV isolates of Maastricht and England, RCMV-E was classified as
Murid herpesvirus 8
by the International Committee on Taxonomy of Viruses.
Complete Genome Sequence of Novel Porcine Epidemic Diarrhea Virus Strain GD-1 in China
Authors: Wei Zhong-yan , Lu Wen-hui , Li Zhi-li , Mo Jian-yue , Zeng Xi-duo , Zeng Zhi-liang , Sun Bao-li , Chen Feng , Xie Qing-Mei , Bee Ying-zuo , Ma Jing-yun 
Published: November 2012

ABSTRACT
Porcine epidemic diarrhea virus (PEDV) infection, which causes acute diarrhea and dehydration in suckling piglets, has become a serious problem for the swine industry of China in recent years. In this study, a virulent PEDV strain, GD-1, was obtained from fecal samples from suckling piglets that suffered from severe diarrhea in 2011 in Guangdong, China. Here we describe the complete genome sequence of strain GD-1, which may be helpful in further understanding the molecular epidemiology and genetic diversity of PEDV field isolates in China.
Genome Sequence of Cronobacter sakazakii Myovirus vB_CsaM_GAP31
Authors: Abbasifar Reza , Kropinski Andrew M. , Sabour Parviz M. , Ackermann Hans-Wolfgang , Alanis Villa Argentina , Abbasifar Arash , Griffiths Mansel W. 
Published: November 2012

ABSTRACT

Cronobacter sakazakii
is a pathogen that predominantly infects immunocompromised individuals, especially infants, where it causes meningitis. The genome of lytic
C. sakazakii
myovirus vB_CsaM_GAP31 has been fully sequenced. It consists of 147,940 bp and has a G+C content of 46.3%. A total of 295 genes, including 269 open reading frames and 26 tRNA genes, were identified. This phage is related to
Salmonella
phage PVP-SE1 and coliphages vB_EcoM-FV3 and rV5.
Complete Genome Sequence of a Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus NM1 Strain from Northern China
Authors: Li Zhenguang , Leng Xue , Qi Qiaoling , Wang Fengxue , Wen Yongjun , Tan Bin , He Yanliang , Xia Mingqi , Lu Wei , Chen Lizhi , Cheng Shipeng , Wu Hua 
Published: November 2012

ABSTRACT
NM1 is a highly pathogenic North American-type porcine reproductive and respiratory syndrome virus (PRRSV). The complete genome sequence shows that NM1 shares high sequence identity (99.2 to 99.4%) to other HP-PRRSV isolates, containing two discontinuous deletions, a 1-amino-acid deletion at position 481 and a 29-amino-acid deletion at positions 533 to 651, in nonstructural protein 2.
Complete Genome Sequence of a Duck Hepatitis A Virus Type 3 Identified in Eastern China
Authors: Xu Qian , Zhang Ruihua , Chen Linlin , Yang Lei , Li Jingxin , Dou Pengfei , Wang Hui , Xie Zhijing , Wang Yu , Jiang Shijin 
Published: November 2012

ABSTRACT
We report here the complete genome sequence of a novel duck hepatitis A virus type 3 (DHAV-3) isolated from a dead Cherry Valley duckling in eastern China. The whole genomic nucleotide sequence and polyprotein amino acid sequence of the virus had higher homology with those of Chinese DHAV-3 isolates, medium homology with those of Korean DHAV-3 isolates, and the lowest homology with those of Vietnamese isolate DN2. The result indicated that the genetic evolution of DHAV-3 isolates had obvious geographical features.
Complete Genome Sequence of a Subgenotype VIId Newcastle Disease Virus Circulating Predominantly in Chickens in China
Authors: Zhang Yuanyuan , Zhang Shouping , Wang Xiaoting , Zhang Guozhong 
Published: November 2012

ABSTRACT
At least 11 genotypes of class II viruses have been identified since the discovery of Newcastle disease virus (NDV) in 1926. Here, we reported the complete genome sequence of a prevalent NDV variant from China, belonging to subgenotype VIId in class II. The similar viruses have been the predominant strains circulating in China for the past decade, which occupied over 80% of Chinese prevalent strains and were phylogenetically different from currently available vaccines.
Complete Genome Sequence of a Vero Cell-Adapted Isolate of Porcine Epidemic Diarrhea Virus in Eastern China
Authors: Zhao Mengjiao , Sun Zhen , Zhang Yue , Wang Guisheng , Wang Hui , Yang Fangfang , Tian Fulin , Jiang Shijin 
Published: November 2012

ABSTRACT
In early 2012, a widespread porcine epidemic diarrhea virus (PEDV) occurred in eastern China. A cell-adapted isolate, SD-M, was at the four-passage level of virulent field strain SD, which was isolated from a 2-day-old dead suckling piglet that had suffered from severe diarrhea in Shandong Province, China. We report here the complete genome sequence of SD-M. This sequence will promote a better understanding of the molecular pathogenesis of PEDV.
Complete Genomic Sequences of Rabbit Hemorrhagic Disease Virus G1 Strains Isolated in the European Rabbit Original Range
Authors: Abrantes Joana , Lopes Ana M. , Esteves Pedro J. 
Published: November 2012

ABSTRACT
The complete genomic sequences of rabbit hemorrhagic disease virus (RHDV) strains isolated in 1995 (CB137) and 2006 (CB194) from wild European rabbits from Portugal are described. The strains were isolated in the original range of the European rabbit and assigned to genogroup 1 (G1), which is known to have persisted only in the Iberian Peninsula. ORF2 of isolate CB137 might encode a shorter minor structural protein, VP10.
Complete Genome Sequence of Porcine Circovirus 2b Strain Shandong
Authors: Xu Shaojian , Li Jun , Yuan Xiaoyuan , Wang Guisheng , Shi Jianli , Wu Jiaqiang , Cong Xiaoyan , Sun Wenbo , Du Yijun , Chai Tongjie , Wang Jinbao 
Published: November 2012

ABSTRACT
Shandong is a porcine circovirus 2b (PCV2b) strain that was isolated and purified from tissue samples from pigs with postweaning multisystemic wasting syndrome (PMWS) in the Shandong Province of China. Here, we report the complete genome sequence of strain Shandong, which may aid in understanding the molecular characteristics of this strain.
At the Edge of Intonation: The Interplay of Utterance-Final F0 Movements and Voiceless Fricative Sounds
Authors: Niebuhr Oliver 
Published: November 2012

AbstractThe paper is concerned with the ‘edge of intonation’ in a twofold sense. It focuses on utterance-final F0 movements and crosses the traditional segment-prosody divide by investigating the interplay of F0 and voiceless fricatives in speech production. An experiment was performed for German with four types of voiceless fricatives: /f/, /s/, /ʃ/ and /x/. They were elicited with scripted dialogues in the contexts of terminal falling statement and high rising question intonations. Acoustic analyses show that fricatives concluding the high rising question intonations had higher mean centres of gravity (CoGs), larger CoG ranges and higher noise energy levels than fricatives concluding the terminal falling statement intonations. The different spectral-energy patterns are suitable to induce percepts of a high ‘aperiodic pitch’ at the end of the questions and of a low ‘aperiodic pitch’ at the end of the statements. The results are discussed with regard to the possible existence of ‘segmental intonation’ and its implication for F0 truncation and the segment-prosody dichotomy, in which segments are the alleged troublemakers for the production and perception of intonation.
Making Sense of Outliers
Authors: Ogden Richard 
Published: November 2012

AbstractPhonetic inquiry, particularly of the kind which looks at large numbers of items, frequently asks what a ‘typical’ example of a phenomenon of speech is like. For example, in describing the plosives of a language, it is common to calculate average closure durations or voice onset times in some contexts. In this paper, the focus is on examples from natural conversation where there are untypical patterns, and where the item of interest is, in statistical terms, an outlier. Outliers are generally excluded from linguistic analysis, and may be treated as e.g. production errors. The paper shows instead that at least some outliers are in fact part of a meaningful practice, and an orderly method by which speakers can create meaning. The phenomenon more precisely is that of intensifying emphasis, which has been described for German. We consider its phonetic exponents in American English, which are both ‘prosodic’ and ‘segmental’. We provide an account of function based on participants’ own use and displayed understanding of the phenomenon, and argue for a structurally rich account of the phenomenon which includes aspects of turn construction and sequential organisation.
Habit and Nonhabit Systems for Unconscious and Conscious Behavior: Implications for Multitasking
Authors: Lisman John , Sternberg Eliezer J. 
Published: November 2012

AbstractThe study of human consciousness has demonstrated that there are both conscious and unconscious systems. Other work, particularly in animals, has shown that there are habit and nonhabit systems and that these involve different brain regions and memory processes. Here we argue that habits can be equated with unconscious behavior and nonhabits with conscious behavior. This equation makes the extensive physiological literature on habit/nonhabit relevant to the less tractable issue of consciousness. On the basis of this line of reasoning, it appears that different parts of the BG and different memory structures mediate conscious and unconscious processes. It is further argued here that the unconscious system is highly capable; it can both process sensory information and produce behavior. The benefit of such a dual system is multitasking: The unconscious system can execute background tasks, leaving the conscious system to perform more difficult tasks.
Dopamine D2 Receptor Modulation of Human Response Inhibition and Error Awareness
Authors: Nandam L. Sanjay , Hester Robert , Wagner Joe , Dean Angela J. , Messer Cassandra , Honeysett Asha , Nathan Pradeep J. , Bellgrove Mark A. 
Published: November 2012

Abstract
Response inhibition, comprising action cancellation and action restraint, and error awareness are executive functions of considerable clinical relevance to neuropsychiatric disorders. Nevertheless, our understanding of their underlying catecholamine mechanisms, particularly regarding dopamine, is limited. Here, we used the dopamine D2 agonist cabergoline to study its ability to improve inhibitory control and modulate awareness of performance errors. A randomized, double-blind, placebo-controlled, crossover design with a single dose of cabergoline (1.25 mg) and placebo (dextrose) was employed in 25 healthy participants. They each performed the stop-signal task, a well-validated measure of action cancellation, and the Error Awareness Task, a go/no-go measure of action restraint and error awareness, under each drug condition. Cabergoline was able to selectively reduce stop-signal RT, compared with placebo, indicative of enhanced action cancellation (p &lt; .05). This enhancement occurred without concomitant changes in overall response speed or RT variability and was not seen for errors of commission on the Error Awareness Task. Awareness of performance errors on the go/no-go task was, however, significantly improved by cabergoline compared with placebo (p &lt; .05). Our results contribute to growing evidence for the dopaminergic control of distinct aspects of human executive ability, namely, action cancellation and error awareness. The findings may aid the development of new, or the repurposing of existing, pharmacotherapy that targets the cognitive dysfunction of psychiatric and neurological disorders. They also provide further evidence that specific cognitive paradigms have correspondingly specific neurochemical bases.
Critical Time Course of Right Frontoparietal Involvement in Mental Number Space
Authors: Rusconi Elena , Dervinis Martynas , Verbruggen Frederick , Chambers Christopher D. 
Published: November 2012

Abstract
Neuropsychological, neurophysiological, and neuroimaging studies suggest that right frontoparietal circuits may be necessary for the processing of mental number space, also known as the mental number line (MNL). Here we sought to specify the critical time course of three nodes that have previously been related to MNL processing: right posterior parietal cortex (rPPC), right FEF (rFEF), and right inferior frontal gyrus (rIFG). The effects of single-pulse TMS delivered at 120% distance-adjusted individual motor threshold were investigated in 21 participants, within a window of 0–400 msec (sampling interval = 33 msec) from the onset of a central digit (1–9, 5 excluded). Pulses were delivered in a random order and with equal probability at each time point, intermixed with noTMS trials. To analyze whether and when TMS interfered with MNL processing, we fitted bimodal Gaussian functions to the observed data and measured effects on changes in the Spatial–Numerical Association of Response Codes (SNARC) effect (i.e., an advantage for left- over right-key responses to small numbers and right- over left-key responses to large numbers) and in overall performance efficiency. We found that, during magnitude judgment with unimanual key-press responses, TMS reduced the SNARC effect in the earlier period of the fitted functions (∼25–60 msec) when delivered over rFEF (small and large numbers) and rIFG (small numbers); TMS further reduced the SNARC effect for small numbers in a later period when delivered to rFEF (∼200 msec). In contrast, TMS of rPPC did not interfere with the SNARC effect but generally reduced performance for small numbers and enhanced it for large numbers, thus producing a pattern reminiscent of “neglect” in mental number space. Our results confirm the causal role of an intact right frontoparietal network in the processing of mental number space. They also indicate that rPPC is specifically tied to explicit number magnitude processing and that rFEF and rIFG contribute to interfacing mental visuospatial codes with lateralized response codes. Overall, our findings suggest that both ventral and dorsal frontoparietal circuits are causally involved and functionally connected in the mapping of numbers to space.