bluuebunny/crossref_metadata_2025_split huggingface dataset:
Excessive Cellular Proliferation Negatively Impacts Reprogramming Efficiency of Human Fibroblasts
Authors: Gupta Manoj K. , Teo Adrian Kee Keong , Rao Tata Nageswara , Bhatt Shweta , Kleinridders Andre , Shirakawa Jun , Takatani Tomozumi , Hu Jiang , De Jesus Dario F. , Windmueller Rebecca , Wagers Amy J. , Kulkarni Rohit N. 
Published: August 2015

Abstract

The impact of somatic cell proliferation rate on induction of pluripotent stem cells remains controversial. Herein, we report that rapid proliferation of human somatic fibroblasts is detrimental to reprogramming efficiency when reprogrammed using a lentiviral vector expressing OCT4, SOX2, KLF4, and cMYC in insulin-rich defined medium. Human fibroblasts grown in this medium showed higher proliferation, enhanced expression of insulin signaling and cell cycle genes, and a switch from glycolytic to oxidative phosphorylation metabolism, but they displayed poor reprogramming efficiency compared with cells grown in normal medium. Thus, in contrast to previous studies, our work reveals an inverse correlation between the proliferation rate of somatic cells and reprogramming efficiency, and also suggests that upregulation of proteins in the growth factor signaling pathway limits the ability to induce pluripotency in human somatic fibroblasts.

Significance
The efficiency with which human cells can be reprogrammed is of interest to stem cell biology. In this study, human fibroblasts cultured in media containing different concentrations of growth factors such as insulin and insulin-like growth factor-1 exhibited variable abilities to proliferate, with consequences on pluripotency. This occurred in part because of changes in the expression of proteins involved in the growth factor signaling pathway, glycolysis, and oxidative phosphorylation. These findings have implications for efficient reprogramming of human cells.
CXCL12Gene Therapy Ameliorates Ischemia-Induced White Matter Injury in Mouse Brain
Authors: Li Yaning , Tang Guanghui , Liu Yanqun , He Xiaosong , Huang Jun , Lin Xiaojie , Zhang Zhijun , Yang Guo-Yuan , Wang Yongting 
Published: August 2015

AbstractRemyelination is an important repair process after ischemic stroke-induced white matter injury. It often fails because of the insufficient recruitment of oligodendrocyte progenitor cells (OPCs) to the demyelinated site or the inefficient differentiation of OPCs to oligodendrocytes. We investigated whether CXCL12 gene therapy promoted remyelination after middle cerebral artery occlusion in adult mice. The results showed that CXCL12 gene therapy at 1 week after ischemia could protect myelin sheath integrity in the perifocal region, increase the number of platelet-derived growth factor receptor-α (PDGFRα)-positive and PDGFRα/bromodeoxyuridine-double positive OPCs in the subventricular zone, and further enhance their migration to the ischemic lesion area. Coadministration of AMD3100, the antagonist for CXCL12 receptor CXCR4, eliminated the beneficial effect of CXCL12 on myelin sheath integrity and negatively influenced OPC proliferation and migration. At 5 weeks after ischemia, CXCR4 was found on the PDGFRα- and/or neuron/glia type 2 (NG2)-positive OPCs but not on the myelin basic protein-positive mature myelin sheaths, and CXCR7 was only expressed on the mature myelin sheath in the ischemic mouse brain. Our data indicated that CXCL12 gene therapy effectively protected white matter and promoted its repair after ischemic injury. The treatment at 1 week after ischemia is effective, suggesting that this strategy has a longer therapeutic time window than the treatments currently available.SignificanceThis study has demonstrated for the first time that CXCL12 gene therapy significantly ameliorates brain ischemia-induced white matter injury and promotes oligodendrocyte progenitor cell proliferation in the subventricular zone and migration to the perifocal area in the ischemic mouse brain. Additional data showed that CXCR4 receptor plays an important role during the proliferation and migration of oligodendrocyte progenitor cells, and CXCR7 might play a role during maturation. In contrast to many experimental studies that provide treatment before ischemic insult, CXCL12 gene therapy was performed 1 week after brain ischemia, which significantly prolonged the therapeutic time window of brain ischemia.
Concise Review: Human Dermis as an Autologous Source of Stem Cells for Tissue Engineering and Regenerative Medicine
Authors: Vapniarsky Natalia , Arzi Boaz , Hu Jerry C. , Nolta Jan A. , Athanasiou Kyriacos A. 
Published: August 2015

Abstract

The exciting potential for regenerating organs from autologous stem cells is on the near horizon, and adult dermis stem cells (DSCs) are particularly appealing because of the ease and relative minimal invasiveness of skin collection. A substantial number of reports have described DSCs and their potential for regenerating tissues from mesenchymal, ectodermal, and endodermal lineages; however, the exact niches of these stem cells in various skin types and their antigenic surface makeup are not yet clearly defined. The multilineage potential of DSCs appears to be similar, despite great variability in isolation and in vitro propagation methods. Despite this great potential, only limited amounts of tissues and clinical applications for organ regeneration have been developed from DSCs. This review summarizes the literature on DSCs regarding their niches and the specific markers they express. The concept of the niches and the differentiation capacity of cells residing in them along particular lineages is discussed. Furthermore, the advantages and disadvantages of widely used methods to demonstrate lineage differentiation are considered. In addition, safety considerations and the most recent advancements in the field of tissue engineering and regeneration using DSCs are discussed. This review concludes with thoughts on how to prospectively approach engineering of tissues and organ regeneration using DSCs. Our expectation is that implementation of the major points highlighted in this review will lead to major advancements in the fields of regenerative medicine and tissue engineering.

Significance
Autologous dermis-derived stem cells are generating great excitement and efforts in the field of regenerative medicine and tissue engineering. The substantial impact of this review lies in its critical coverage of the available literature and in providing insight regarding niches, characteristics, and isolation methods of stem cells derived from the human dermis. Furthermore, it provides analysis of the current state-of-the-art regenerative approaches using human-derived dermal stem cells, with consideration of current guidelines, to assist translation toward therapeutic use.
Scientific production about calgary model for evaluation of the family: a bibliometric study
Authors: Rodrigues Francileide de Araújo , Costa Solange Fátima Geraldo da , Fernandes Maria Andréia , Zaccara Ana Aline Lacet , Duarte Marcella Costa Souto , Andrade Cristiane Garrido de 
Published: August 2015

Objetivo: Caracterizar a produção científica disponibilizada em periódicos online sobre o Modelo Calgary de Avaliação da Família. Métodos: Pesquisa bibliométrica cuja amostra correspondeu 52 artigos publicados entre 2004 e 2013 em periódicos de enfermagem, nas bases de dados LILACS, BDENF e na Biblioteca Digital SciELO. Dados coletados de março a abril de 2014. Resultados: Os dados evidenciaram que em 2009 teve o maior quantitativo de trabalhos (15.38%). Os periódicos com maior número de publicações foram a Revista de Pesquisa: Cuidado é Fundamental e a Revista da Escola Anna Nery Revista de Enfermagem. O maior quântico dos artigos obtiveram Qualis B2 e B1, com destaque para base de dados LILACS. Quanto à modalidade das publicações predominaram estudos originais. Ressalta-se a titulação de mestre entre os pesquisadores dos estudos. Conclusão: Os resultados evidenciaram a produção científica investigada como uma temática de interesse e crescimento no campo da Enfermagem.
An analytical model of sub‐Alfvénic moon‐plasma interactions with application to the hemisphere coupling effect
Authors: Simon Sven 
Published: August 2015

AbstractWe develop a new analytical model of the Alfvén wing that is generated by the interaction between a planetary moon's ionosphere and its magnetospheric environment. While preceding analytical approaches assumed the obstacle's height‐integrated ionospheric conductivities to be spatially constant, the model presented here can take into account a continuous conductance profile that follows a power law. The electric potential in the interaction region, determining the electromagnetic fields of the Alfvén wing, can then be calculated from an Euler‐type differential equation. In this way, the model allows to include a realistic representation of the “suspension bridge”‐like conductance profile expected for the moon's ionosphere. The major drawback of this approach is its restriction to interaction scenarios where the ionospheric Pedersen conductance is large compared to the Hall conductance, and thus, the Alfvénic perturbations are approximately symmetric between the planet‐facing and the planet‐averted hemispheres of the moon. The model is applied to the hemisphere coupling effect observed at Enceladus, i.e., to the surface currents and the associated magnetic discontinuities that arise from a north‐south asymmetry of the obstacle to the plasma flow. We show that the occurrence of this effect is very robust against changes in the conductance profile of Enceladus' plume, and we derive upper limits for the strength of the magnetic field jumps generated by the hemisphere coupling effect. During all 11 reported detections of the hemisphere coupling currents at Enceladus, the observed magnetic field jumps were clearly weaker than the proposed limits. Our findings are also relevant for future in situ studies of putative plumes at the Jovian moon Europa.
Mechanical compaction and strain localization in Bleurswiller sandstone
Authors: Baud Patrick , Reuschlé Thierry , Ji Yuntao , Cheung Cecilia S. N. , Wong Teng‐fong 
Published: August 2015

AbstractWe performed a systematic investigation of mechanical compaction and strain localization in Bleurswiller sandstone. Our data show that the effective pressure principle can be applied in both the brittle faulting and cataclastic flow regimes, with an effective pressure coefficient close to but somewhat less than 1. Under relatively high confinement, the samples typically fail by development of compaction bands. X‐ray computed tomography (CT) was used to resolve preexisting porosity clusters, as well as the initiation and propagation of the compaction bands in deformed samples. Synthesis of the CT and microstructural data indicates that there is no casual relation between collapse of the porosity clusters in Bleurswiller sandstone and nucleation of the compaction bands. Instead, the collapsed porosity clusters may represent barriers for the propagation of compaction localization, rendering the compaction bands to propagate along relatively tortuous paths so as to avoid the porosity clusters. The diffuse and tortuous geometry of compaction bands results in permeability reduction that is significantly lower than that associated with compaction band formation in other porous sandstones. Our new data confirm that Bleurswiller sandstone stands out as the only porous sandstone associated with a compactive cap that is linear, and our CT and microstructural observation show that it is intimately related to collapse of the porosity clusters. We demonstrate that the anomalous linear caps and their slopes are in agreement with a micromechanical model based on the collapse of a spherical pore embedded in an elastic‐plastic matrix that obeys the Coulomb failure criterion.
Trends in chronic rhinosinusitis research in the past three decades
Authors: Banglawala Sarfaraz M. , Schlosser Rodney J. , Wenztel Jennifer , Walsh Thomas , Overton Lewis , Soler Zachary M. 
Published: August 2015

BackgroundThe objective of this work was to identify trends in chronic rhinosinusitis (CRS)‐related publications for the past 3 decades.MethodsLiterature review was conducted using multiple terms, including sinusitis, chronic rhinosinusitis, chronic sinus disease, nasal polyposis, ethmoid sinusitis, frontal sinusitis, and maxillary sinusitis. Abstracts were divided into 3 decades: 1983 through 1992, 1993 through 2002, and 2003 through 2012. For each decade, we compared the total number of publications and journals, study design, use of validated outcome measures, quality of evidence, number of authors, country of origin, and clinical vs basic science.ResultsA total of 3406 abstracts were identified. There was a statistically significant increase in the number of publications with a 637% increase from 1983 through 1992 to 2003 through 2012 (p < 0.05). Likewise, the number of journals with CRS‐related publications significantly increased during the study period (117 to 350; p < 0.05). Prospective studies increased (15.30% to 28.90%, p < 0.05) and retrospective studies decreased (33.00% to 17.36%, p < 0.05). Cohort studies were the most common type of design study (18.70% to 32.46%). In studies reporting outcome measures, the use of validated measures significantly increased over time (2.56% to 49.70%, p < 0.05). Although, the quality of evidence for most clinical publications for all 3 decades were grade C, the number and percentage of grade A and grade B publications increased significantly over time (0.99% to 7.23%, p < 0.05; 6.9% to 10.44%, p < 0.05; respectively).ConclusionCRS‐related publication quantity and quality have increased over the last 3 decades.
The clinical significance of the pollen calendar of the Western Thrace/northeast Greece region in allergic rhinitis
Authors: Katotomichelakis Michael , Nikolaidis Christos , Makris Michael , Zhang Nan , Aggelides Xenophon , Constantinidis Theodoros C. , Bachert Claus , Danielides Vassilios 
Published: August 2015

BackgroundThere are major differences in the clinical pattern of allergic rhinitis (AR) patients among countries, reflecting local aerobiological conditions. We analyzed the correlation between airborne pollen concentrations of the Western Thrace/northeast (NE) Greece region with symptoms scores in AR patients. The above data is the first provided for the Mediterranean climate of Greece and reflects the clinical significance of pollen calendar in everyday clinical practice.MethodsAn annual pollen calendar of the most important outdoor aeroallergens (grasses, trees, and weeds) was developed, using a Burkard volumetric spore trap. A total of 168 AR patients were studied and asked to evaluate their symptoms and main seasonal appearance. Sensitization prevalence to pollen species was detected by skin prick tests. Symptoms were evaluated by Total 5 Symptoms Score (T5SS) and correlated to aerobiological data.ResultsAs far as the pollen calendar is concerned, the highest total percentages of pollens were recorded for olive (24.02% of total), oak (13.74%), grasses (9.08%), and cypress (7.63%). Regarding patients’ sensitivities, the most prevalent ones were to grasses (56.0%), olive (43.5%), wall pellitory (24.4%), and cypress (16.7%) antigens. A strong significant correlation between total pollen counts and patients’ T5SS (r = 0.874, p < 0.001) was observed. Moreover, strong significant correlations between T5SS and pollen counts were also found for the most prevalent species, including grasses, olive, and Parietaria allergens (r = 0.627, p = 0.029; r = 0.695, p = 0.012; and r = 0.656, p = 0.021, respectively).ConclusionPatients’ symptoms scores were found to be significantly correlated to pollen counts. Given data are important for the management of AR patients who live in similar Mediterranean climate conditions.
Condensation Agents Determine the Temperature–Pressure Stability of F‐Actin Bundles
Authors: Gao Mimi , Berghaus Melanie , von der Ecken Julian , Raunser Stefan , Winter Roland 
Published: August 2015

AbstractBiological cells provide a large variety of rodlike filaments, including filamentous actin (F‐actin), which can form meshworks and bundles. One key question remaining in the characterization of such network structures revolves around the temperature and pressure stabilities of these architectures as a way to understand why cells actively use proteins for forming them. The packing properties of F‐actin in fascin‐ and Mg2+‐induced bundles are compared, and significantly different pressure‐temperature stabilities are observed because of marked differences in their nature of interaction, solvation, and packing efficiency. Moreover, differences are observed in their morphologies and disintegration scenarios. The pressure‐induced dissociation of the actin bundles is reminiscent of a single unbinding transition as observed in other soft elastic manifolds.
Development of polyvinyl alcohol/intercalated MMT composite foams fabricated by melt extrusion
Authors: Li Yan , Tian Huafeng , Jia Qingqing , Niu Ping , Xiang Aimin , Liu Di , Qin Yanan 
Published: August 2015

ABSTRACTIn this work, novel polyvinyl alcohol (PVA)/intercalated montmorillonite (MMT) nanocomposite foams were fabricated by melt extrusion with fixed amount of foaming agent, and the structure and properties were investigated with X‐ray diffraction, transmittance electron microscope, differential scanning calorimetry, plate rheometer, scanning electron microscope, density analysis, and mechanical testing in detail. The results revealed that pristine MMT could be intercalated by PVA during melt‐extrusion. Both exfoliation and intercalation structure was formed in the composites. The strong interaction between PVA and MMT restricted the motion of PVA molecules, leading to the enhanced complex viscosity, storage modulus, and melt flow index, suggesting the improved melt elasticity and melt strength. The improved melt strength of PVA through MMT incorporation facilitated the foaming process. With the increase of MMT, cell rupture was limited and the thickness of cell‐wall decreased, leading to the decreased density. The homogeneous cells and the closed cell structure with the foaming density of 0.35 g/cm3 could be formed with 2 wt % MMT incorporation. With the increase of MMT, the tensile strength of PVA/MMT foams decreased because of the enlarged foaming ratio and decreased density. This work aims to pave a simple and convenient way to produce biodegradable foams. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42706.
Role of silk fibroin and rice starch on some physical properties of hydroxyapatite‐based composites
Authors: Sriudom Suchaya , Niamsup Hataichanoke , Saipanya Surin , Watanesk Ruangsri , Watanesk Surasak 
Published: August 2015

ABSTRACTThe role of organic blends of silk fibroin (SF) and rice starch (RS) in bone composites based on inorganic hydroxyapatite (HA) is studied. The physical property of HA‐based composites prepared by using the sol–gel method from Ca(OH)2 and H3PO4 in ethanol and water solvent (4 : 1 volume ratio) could be improved by adding SF and RS (1 : 2 weight ratio) to HA (7 : 3 weight ratio). The Fourier transform infrared spectrometer spectrum shows that the SF and RS organic phases are blended homogeneously into the HA crystal structure. Addition of SF increases the pore size and surface area of the composites, as measured by Brunauer–Emmett–Teller method, but their pore volume is slightly decreased. The values of lattice parameters, crystallinity, and crystallite size, as determined from the field‐emission scanning electron microscope, transmission electron microscope, and X‐ray diffractometer results, increase after adding RS. The results are agreeable with the increase of their compressive strength and Young's modulus. Thus, the improved physical property of the prepared HA–SF/RS composites is better suited as bone‐filling material than the standard HA or HA‐based composites with either SF or RS only. Therefore, due to its low cost, biocompatibility, and nontoxicity, this innovative solution could be worth taken under consideration by the restorative dental and orthopedic implants industry. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42722.
Electrospun protective self‐healing coatings for light alloys: A better understanding of the intrinsic potential of the technology
Authors: Firouzi Amin , Impagnatiello Andrea , Del Gaudio Costantino , Lamastra Francesca Romana , Bianco Alessandra , Montesperelli Giampiero 
Published: August 2015

ABSTRACTPolymeric coating systems exhibit high potentiality to provide an effective barrier against corrosion of metallic surfaces. However, these coatings can lose their protective characteristics because of their high susceptibility to damage. Thus, the addition of corrosion inhibitors is desirable and considered as an alternative route for active corrosion protection. In the present work, eco‐friendly electrospun coatings of poly(vinyl alcohol) (PVA) loaded with cerium salts have been deposited onto aluminium 6082 alloy. Two different precursors of cerium (III) (i.e., cerium nitrate and cerium acetylacetonate) were added to the electrospinning solutions and the effectiveness of the resulting nanofibrous coatings was evaluated for the healing of generated defects. The microstructural features of the electrospun coatings have been investigated by scanning electron microscopy, infraredspectroscopy, and thermal analysis. Tensile tests were performed to assess the mechanical properties of the different fibrous coatings. The electrochemical behavior of both intact and damaged coatings was evaluated in 3 wt % NaCl solution by means of electrochemical impedance spectroscopy. All the deposited PVA coatings loaded with cerium(III) salts showed remarkable corrosion resistance. In the case of artificially damaged coatings, a self‐healing effect, which stops the development of the corrosion process and provides a significant recovery of the corrosion resistance, has been observed only for coatings loaded with cerium III acetylacetonate. The release of cerium from damaged PVA fibers has been demonstrated by means of inductively coupled plasma mass spectrometry. The observed self‐healing effect has been ascribed to the formation of cerium hydroxide on the defective zone, which hindered the corrosion process. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42728.
Effect of zinc oxide on properties of phenolic foams/halogen‐free flame retardant system
Authors: Ma Yufeng , Wang Jifu , Xu Yuzhi , Wang Chunpeng , Chu Fuxiang 
Published: August 2015

ABSTRACTA halogen‐free flame retardant system consisting of ammonium polyphosphate (APP) as an acid source, blowing agent, pentaerythritol (PER) as a carbonific agent and zinc oxide (ZnO) as a synergistic agent, was used in this work to enhance flame retardancy of phenolic foams. ZnO was incorporated into flame retardant formulation at different concentrations to investigate the flammability of flame retardant composite phenolic foams (FRCPFs). The synergistic effects of ZnO on FRCPFs were evaluated by limited oxygen index (LOI), thermogravimetric analysis (TGA), cone calorimeter tests, and images of residues. Results showed that the flame retardant significantly increased the LOI of FRCPFs. Compared with PF, heat release rate (HRR), total heat release (THR), effective heat of combustion (EHC), production or yield of carbon monoxide (COP or COY) and Oxygen consumption (O2C) of FRCPFs all remarkably decreased. However specific extinction area (SEA) and total smoke release (TSR) significantly increased, which agreed with the gas‐phase flame retardancy mechanism of the flame retardant system. The results indicated that FRCPFs have excellent fire‐retardant performance and less smoke release. And the bending and compression strength were decreased gradually with the increase of ZnO. The comprehensive properties of FRCPFs were better when the amount of ZnO was 1∼1.5%. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42730.