bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of root canal morphology in maxillary premolars among the Pakistani subpopulation: a CBCT-based study
Authors: Algarni Hmoud Ali , Alonazi Meshal Aber , Arshad Hamza , Zahra Fatima , Umer Fahad , Maqbool Irfan , Iqbal Azhar , Karobari Mohmed Isaqali 
Published: July 2024

Abstract
Background
Understanding the root canal morphology is essential for the success of root canal treatment. Therefore, this study aimed to evaluate and analyze the root canal configuration of maxillary premolars using Cone Beam Computed Tomography in the Pakistani subpopulation.

Method
This cross-sectional study utilized CBCT scans from two distinct centres: Aga Khan University in Karachi and Jinnah MRI and Body Scans in Lahore. The CBCT images were visualized using GALAXIS version 1.9 (SICAT GmbH and Co. KG, Bonn, Germany), integrated within the Sirona Dental System (D-64625 Bensheim, Germany). The scanning parameters were standardized at 85 kV, 7 mA, with a 15-s exposure time and a voxel size of 0.16 mm. A total of 707 CBCT scans were collected, encompassing 2180 maxillary premolars. Root canal configurations were classified based on (Ahmed et al. Int Endod J. 2017;50(8):761–70). Statistical analyses were performed using SPSS version 26, employing the Chi-square test with a significance level set at p < 0.05.

Results
The distribution of root canal morphologies varied significantly with age and gender. Among maxillary premolars, 50% exhibited the typical configuration of 2MPMB1 L1 (two roots, single canal in each buccal and lingual root), while 26% of maxillary right second premolars displayed 1MPM1 (one root, one canal). Overall, 1MPM1 accounted for 27.4% of the total cases in the second premolars. There was no statistically significant relationship between age and root canal distribution in either first premolars (p = 0.338) or second premolars (p = 0.833). Regarding gender, a significant difference was observed in the distribution of right maxillary 1st premolars (p = 0.022*), with a higher prevalence among females.

Conclusion
This study offers significant insights into the anatomical variations of root canals in maxillary premolars across diverse regional subpopulations in Pakistan. While specific root canal configurations were prevalent, the findings indicate no statistically significant correlation between age and root canal morphology in maxillary premolars. However, a notable gender disparity was observed in the distribution of the right maxillary first premolars.
The perceived social support of parents having bipolar disorder impacts their children’s mental health: a 10-year longitudinal study
Authors: Trespalacios Florencia , Boyle Ariel , Serravalle Lisa , Hodgins Sheilagh , Ellenbogen Mark A. 
Published: July 2024

Abstract
Background
The offspring of parents with bipolar disorder (OBD) are at higher risk of developing psychopathology than the offspring of parents with no affective disorder (control). In addition to genetic predisposition, childhood adversity and a stressful family environment are important risk factors for the OBD. Protective factors in parents, such as social support and coping strategies, may buffer the effects of stress on at-risk children. This study tested whether parents’ social support and coping style attenuated the link between risk status (OBD vs. control) and psychopathology in offspring.

Methods
During offspring’s middle childhood, parents underwent a diagnostic interview and completed social support and coping style questionnaires. Sixty-nine OBD (39 female) and 69 control (29 female) offspring between ages 13 and 29 completed a diagnostic interview approximately 10 years later.

Results
Parents’ social support satisfaction moderated the link between offspring risk status and their development of substance use disorder (SUD) symptoms (F(1,131) = 5.90, p = .017). Parents’ social network size moderated the link between offspring risk status and their development of anxiety and depression symptoms in an unexpected direction (F(1,131) = 5.07, p = .026). No effects of parents’ coping style were found.

Conclusions
Among the OBD, having parents with greater social support satisfaction and, unexpectedly, a smaller social network buffered their development of SUD and depression and anxiety symptoms by early adulthood. Parents’ social support may thus have a protective function for children in these high-risk families.
A fungus-based soil improvement using Rhizopus oryzae inoculum
Authors: Jerez Lazo Cristian , Lee Nathan , Tripathi Priya , Joykutty Leya , Jayachandran Krishnaswamy , Lee Seung Jae 
Published: July 2024

AbstractThis study demonstrates the efficacy of employing Rhizopus oryzae fungus inoculum as a potential solution to improve soil erodibility in coastal environments. A set of unconfined compression tests is conducted on Miami Beach sand treated with a R. oryzae inoculum. Our findings suggest that the R. oryzae fungus inoculum effectively improves the stability of sand by acting as a natural binding agent. This finding aligns with previous studies that utilized different Rhizopus species, such as Rhizopus oligosporus, to improve sand properties. However, a notable difference is observed; the R. oryzae-treated sand exhibits remarkable durability, maintaining significant strength over an extended period without water or dietary supply. The durability is likely attributable to the morphological characteristics of R. oryzae that extensively branches its mycelial network. This paper shares the new discovery to the bio-geotechnics research community, potentially allowing for the customization of soil improvement process by choosing between the fast-acting R. oligosporus and the longer-lasting R. oryzae.
Distinct changes in tomato-associated multi-kingdom microbiomes during Meloidogyne incognita parasitism
Authors: Kudjordjie Enoch Narh , Santos Susana S , Topalović Olivera , Vestergård Mette 
Published: July 2024

Abstract
Background
The interplay between root-knot nematode (RKN) parasitism and the complex web of host-associated microbiota has been recognized as pivotal for effective management of the pest. However, studies assessing this relationship have focussed on the bacterial and fungal communities, neglecting the unicellular eukaryotic members. Here, we employed amplicon sequencing analysis of the bacterial 16S rRNA, fungal ITS and eukaryotic 18S rRNA genes, and comprehensively examined how the microbiome composition, diversity and networking developed with time in the rhizospheres and roots of RKN-inoculated and non-inoculated tomato plants.

Results
As expected, infection with the RKN Meloidogyne incognita decreased plant growth. At individual timepoints, we found distinct bacterial, fungal and eukaryote community structures in the RKN-inoculated and non-inoculated rhizospheres and roots, and RKN inoculation affected several taxa in the root-associated microbiome differentially. Correlation analysis revealed several bacterial and fungal and few protist taxa that correlated negatively or positively with M. incognita. Moreover, network analysis using bacterial, fungal and eukaryotic data revealed more dynamic networks with higher robustness to disturbances in the RKN-inoculated than in the non-inoculated rhizospheres/roots. Hub taxa displayed a noticeable successional pattern that coincided with different phases of M. incognita parasitism. We found that fungal hubs had strong negative correlations with bacteria and eukaryotes, while positive correlations characterized hub members within individual kingdoms.

Conclusion
Our results reveal dynamic tomato-associated microbiomes that develop along different trajectories in plants suffering M. incognita infestation and non-infested plants. Overall, the results identify stronger associations between RKN and bacterial and fungal taxa than between eukaryotic taxa and RKN, suggesting that fungal and bacterial communities could play a larger role in the regulation of RKN. The study identifies several putative RKN-antagonistic bacterial and fungal taxa and confirms the antagonistic potential previously identified in other taxa.
Evaluating the value of individualized 3D printed models for examination, diagnosis and treatment planning of cervical cancer
Authors: Scherer-Quenzer Anne Cathrine , Beyers Inga , Kalisz Adam , Sauer Stephanie Tina , Zimmermann Marcus , Wöckel Achim , Polat Bülent , Schlaiss Tanja , Schelbert Selina , Kiesel Matthias 
Published: July 2024

Abstract
Background
3D printing holds great potential of improving examination, diagnosis and treatment planning as well as interprofessional communication in the field of gynecological oncology. In the current manuscript we evaluated five individualized, patient-specific models of cervical cancer FIGO Stage I-III, created with 3D printing, concerning their value for translational oncology.

Methods
Magnetic resonance imaging (MRI) of the pelvis was performed on a 3.0 Tesla MRI, including a T2-weighted isotropic 3D sequence. The MRI images were segmented and transferred to virtual 3D models via a custom-built 3D-model generation pipeline and printed by material extrusion. The 3D models were evaluated by all medical specialties involved in patient care of cervical cancer, namely surgeons, radiologists, pathologists and radiation oncologists. Information was obtained from evaluated profession-specific questionnaires which were filled out after inspecting all five models. The questionnaires included multiple-select questions, questions based on Likert scales (1 = „strongly disagree “ or „not at all useful “ up to 5 = „strongly agree “ or „extremely useful “) and dichotomous questions (“Yes” or “No”).

Results
Surgeons rated the models as useful during surgery (4.0 out of 5) and for patient communication (4.7 out of 5). Furthermore, they believed that the models had the potential to revise the patients’ treatment plan (3.7 out of 5). Pathologists evaluated with mean ratings of 3.0 out of 5 for the usefulness of the models in diagnostic reporting and macroscopic evaluation. Radiologist acknowledged the possibility of providing additional information compared to imaging alone (3.7 out of 5). Radiation oncologists strongly supported the concept by rating the models highly for understanding patient-specific pathological characteristics (4.3 out of 5), assisting interprofessional communication (mean 4.3 out of 5) and communication with patients (4.7 out of 5). They also found the models useful for improving radiotherapy treatment planning (4.3 out of 5).

Conclusion
The study revealed that the 3D printed models were generally well-received by all medical disciplines, with radiation oncologists showing particularly strong support. Addressing the concerns and tailoring the use of 3D models to the specific needs of each medical speciality will be essential for realizing their full potential in clinical practice.
Computational analysis of the divergent neurotranscriptomic signatures of major depression and suicidality
Authors: Nishanth M. J. , Karthick S. Sai , Jha Shanker 
Published: July 2024

Abstract
Background
Suicide is a leading cause of death globally. Identifying individuals at higher suicidal risk is a key to suicide prevention. Patients with comorbid psychiatric disorders, especially major depressive disorder (MDD), are known to be highly prone to suicidality. However, the behavioural manifestations of MDD and suicidality are distinct, indicating potentially unique molecular underpinnings, of which our current understanding is limited. Delineating the unique and shared molecular etiologies of MDD and suicidality would be imperative to devise effective treatment strategies for suicidal behaviour. To this end, we analysed the existing literature pertaining to transcriptomic alterations in brain samples of individuals who died from MDD or by suicide. Subsequently, biological processes associated with the differentially expressed genes (DEGs) were identified. In addition, we also examined the transcriptional regulators (TRs) potentially driving cortical gene expression changes in MDD and suicidality.

Results
A set of immunological genes was found to be commonly upregulated in MDD but downregulated in suicide. Actin and cytoskeleton organization genes also had a similar trend. In addition, MDD-upregulated and suicide-downregulated genes were found to have overrepresented target sites for 40 TRs associated with epigenetic as well as polymerase-mediated regulation. Any variations in the levels of these TRs could be of behavioural consequence in MDD and suicidality.

Conclusions
A clear understanding of the condition-specific neurotranscriptomic differences in MDD and suicidality would be valuable in order to delineate the biological mechanisms underlying these conditions. Importantly, it would provide insights into more effective treatment strategies for suicidality among individuals with or without MDD. However, we have yet to determine the molecular basis of suicidality in the context of MDD and as a standalone mental condition. In this regard, the present findings would be of scientific and clinical relevance and could stimulate further research.
Novel single nucleotide polymorphism G216A of hormone-sensitive lipase gene associated with Awassi sheep reproduction
Authors: Mohammed Suroor H. , Al-Thuwaini Tahreer M. 
Published: July 2024

Abstract
Background
Litter size plays a crucial role in determining profitability in the sheep industry. Breeding sheep with high litter sizes could be enhanced by selecting candidate genes. One gene affecting sheep's reproductive performance is the hormone-sensitive lipase (HSL) gene. As a result, this study investigated whether the HSL gene variation influenced the fertility of Awassi ewes. The genomic DNA was extracted from 52 singleton ewes and 48 twin ewes. The HSL gene exon 9 (278 bp) was amplified using polymerase chain reaction (PCR).

Results
Study results revealed two genotypes identified in the 278-bp amplicons: GG and GA. Molecular sequence analysis identified a novel mutation in the GA genotype 216G > A. The statistical analysis revealed a significant association between the single nucleotide polymorphism (SNP) 216A > G and reproductive performance. Ewes with the SNP 216G > A genotype exhibited significantly increased litter sizes, twinning rates, lambing rates, and fewer days to lambing compared to ewes with GG genotypes (P ≤ 0.05). The logistic regression analysis results provided strong evidence that the 216G > A mutation significantly increased litter sizes.

Conclusions
This study concluded that variant 216G > A SNP positively impacts Awassi sheep reproduction. There is a higher litter size and more prolificacy in ewes with the 216G > A SNP than in those without the SNP.
How HR analytics evolved over time: a bibliometric analysis on Scopus database
Authors: Sakib Md. Nazmus , Chowdhury Shah Ridwan , Younus Mohammad , Sanju Nehad Laila , Satata Farhana Foysal , Islam Mahafuza 
Published: July 2024

AbstractOver the last decade, human resource (HR) analytics has been widely discussed in the landscape of human resource management due to its dynamic capacity to transform into a data-driven decision-making system for optimizing workforce management. The aim of this paper is to provide a comprehensive summary of the emerging trends and themes of HR analytics drawn from previous literature and offer valuable insight into academia, researchers, practitioners, and policymakers. This paper employs a bibliometric methodology while using RStudio, Biblioshiny, and VOSviewer tools to collect and analyze 102 articles from the Scopus database from January 2008 to September 2023. The findings of the paper reveal current state-of-the-art research in the HR analytics domain while exploring key themes and areas for further study. This study offers practical guidelines for policymakers and contributes to the existing knowledge domain of HR analytics.
Enhancing crop productivity and its economic farm profitabilty of smallholder farmer through the use of green manures from
Alnus acuminata
Authors: Musana Bernard Segatagara , Nabahungu Nsharwasi Léon , Bucagu Charles , Mukuralinda Athanase , Barrios Edmundo , Rutebuka Jules , Nyaga John , Muthuri Catherine Wangari 
Published: July 2024

Abstract

Decline in soil fertility is a major threat to land productivity and food security in the East African highlands. This calls for the application of nutrient inputs to improve crop productivity. A study was conducted in Nyabihu District of Rwanda from 2013 to 2016 to assess the effect of
Alnus acuminata
green manure (AGM)─applied in situ, through biomass alone, or combined with inorganic fertilizer─on the productivity and profitability of maize (
Zea mays
), beans (
Phaseolus vulgaris
), and potato (
Solanum tuberosum
). The treatments included application of AGM, inorganic fertilizer, combination of AGM and inorganic fertilizer, and unfertilized plot as a control (except for potato). These treatments were compared in two seasons and on two local soil fertility levels (medium and high) as defined by the farmer’s knowledge and experience. There was a convergence between farmers’ criteria and soil analysis in the soil fertility evaluation. Crops yields were analyzed using a linear mixed model while for other parameters, descriptive statistics were applied. The combination of AGM and inorganic fertilizer recorded the highest increment in maize (44%) and bean (46%) yields compared with inorganic fertilizer while it increased up to 87% compared with the unfertilized control. The financial analysis showed that AGM + inorganic fertilizer recorded the highest value-to-cost ratio (VCR) of 24.6 for potato and a significantly lower VCR (2.9) for maize and beans. The high VCR highlights a significant potential contribution of AGM + inorganic fertilizer to increase incomes of resource-constrained potato farmers in the Rwandan highlands. However, low crop prices possibly make this practice less attractive for those cultivating maize and beans.
Reclaiming urban lost spaces around elevated urban linkages: the case of El Sawy Cultural Wheel, Egypt
Authors: Nasr Ahmed , El Temsahi Ola , Sarhan Alaa El Din 
Published: July 2024

AbstractIn recent times, elevated urban linkages (EULs) have emerged as crucial components of urban development, cutting through the cities’ urban fabric to accommodate the rapid expansion of urban cores and to facilitate traffic flow. However, these massive infrastructures have inadvertently given rise to urban lost spaces—vacant, inefficient areas within the urban fabric. Recognizing these lost spaces as untapped public assets, the paper explores sustainable development strategies to reclaim them into the urban fabric, transforming them into vibrant, multi-functional public spaces. The paper aims to achieve a set of urban design guidelines for rehabilitation design interventions which will be deduced into a comprehensive framework. This framework encompasses criteria for selecting appropriate approaches and types of rehabilitation design interventions, as well as design principles ensuring the desirability of the reclaimed space. The paper applies this framework to assess the El Sawy Cultural Wheel in Egypt, revealing it as a successful example of a reclaimed space. While positive aspects of rehabilitation design interventions are evident in terms of the approach and type of intervention in this case study, there are identified areas with shortcomings in fully implementing the design principles, if enhanced, could elevate the overall quality of the reclaimed space.
High-throughput Screening for Cushing Disease: Therapeutic Potential of Thiostrepton via Cell Cycle Regulation
Authors: Hakata Takuro , Yamauchi Ichiro , Kosugi Daisuke , Sugawa Taku , Fujita Haruka , Okamoto Kentaro , Ueda Yohei , Fujii Toshihito , Taura Daisuke , Inagaki Nobuya 
Published: July 2024

Abstract
Cushing disease is a life-threatening disorder caused by autonomous secretion of ACTH from pituitary neuroendocrine tumors (PitNETs). Few drugs are indicated for inoperative Cushing disease, in particular that due to aggressive PitNETs. To explore agents that regulate ACTH-secreting PitNETs, we conducted high-throughput screening (HTS) using AtT-20, a murine pituitary tumor cell line characterized by ACTH secretion. For the HTS, we constructed a live cell–based ACTH reporter assay for high-throughput evaluation of ACTH changes. This assay was based on HEK293T cells overexpressing components of the ACTH receptor and a fluorescent cAMP biosensor, with high-throughput acquisition of fluorescence images. We treated AtT-20 cells with compounds and assessed ACTH concentrations in the conditioned media using the reporter assay. Of 2480 screened bioactive compounds, over 50% inhibition of ACTH secreted from AtT-20 cells was seen with 84 compounds at 10 μM and 20 compounds at 1 μM. Among these hit compounds, we focused on thiostrepton (TS) and determined its antitumor effects in both in vitro and in vivo xenograft models of Cushing disease. Transcriptome and flow cytometry analyses revealed that TS administration induced AtT-20 cell cycle arrest at the G2/M phase, which was mediated by FOXM1-independent mechanisms including downregulation of cyclins. Simultaneous TS administration with a cyclin-dependent kinase 4/6 inhibitor that affected the cell cycle at the G0/1 phase showed cooperative antitumor effects. Thus, TS is a promising therapeutic agent for Cushing disease. Our list of hit compounds and new mechanistic insights into TS effects serve as a valuable foundation for future research.
Distinct Indications for Adjuvant Therapy in Resected Invasive Mucinous Cystic Neoplasms of the Pancreas Compared with Pancreatic Ductal Adenocarcinoma
Authors: Wong Paul , Pollini Tommaso , Adam Mohamed A. , Alseidi Adnan , Corvera Carlos U. , Hirose Kenzo , Kirkwood Kimberly S. , Nakakura Eric K. , Thornblade Lucas , Maker Ajay V. 
Published: July 2024

Abstract
Background
Surgical and adjuvant management of mucinous cystic neoplasms (MCNs) lacks formal guidelines and data is limited to institutional studies. Factors associated with receipt of adjuvant therapy and any associated impact on survival remain to be clarified. In the absence of other data, guidelines that recommend adjuvant chemotherapy for invasive pancreatic adenocarcinoma have been extrapolated to MCN.

Patients and Methods
The National Cancer Database (2004–2019) was utilized to identify all patients that underwent pancreatic resection for invasive MCNs. Patients that received neoadjuvant therapy or did not undergo lymphadenectomy were excluded. Patient, tumor, and treatment factors associated with survival were assessed.

Results
For 161 patients with invasive MCN, median overall survival (OS) was 133 months and 45% of patients received adjuvant therapy. Multivariable analysis demonstrated that poorly differentiated tumors [odds ratio (OR) 4.19, 95% confidence interval (CI) 1.47–11.98; p = 0.008] and positive lymph node status (OR 2.67, 95% CI 1.02–6.98; p = 0.042) were independent predictors of receiving adjuvant therapy. Lymph node positivity [hazard ratio (HR) 2.90, 95% CI 1.47–5.73; p = 0.002], positive margins (HR 5.28, 95% CI 2.28–12.27; p < 0.001), and stage III disease (HR 12.46, 95% CI 1.40–111.05; p = 0.024) were associated with worse OS. Receipt of adjuvant systemic therapy was independently associated with decreased risk of mortality in node positive patients (HR 0.23, 95% CI 0.10–0.69; p = 0.002). Survival was not associated with adjuvant therapy in patients with negative lymph nodes or margin negative status.

Conclusion
In contrast to pancreatic ductal adenocarcinoma (PDAC), where adjuvant therapy improves OS for every tumor stage, surgery alone for invasive MCN is not associated with improved OS compared with surgery plus adjuvant therapy in node-negative patients. Surgery alone is likely sufficient for a subset of invasive MCN.