bluuebunny/crossref_metadata_2025_split huggingface dataset:
The cancer gene WWOX behaves as an inhibitor of SMAD3 transcriptional activity via direct binding
Authors: Ferguson Brent W , Gao Xinsheng , Zelazowski Maciej J , Lee Jaeho , Jeter Collene R , Abba Martin C , Aldaz C Marcelo 
Published: December 2013

Abstract

Background
The WW domain containing protein WWOX has been postulated to behave as a tumor suppressor in breast and other cancers. Expression of this protein is lost in over 70% of ER negative tumors. This prompted us to investigate the phenotypic and gene expression effects of loss of WWOX expression in breast cells.

Methods
Gene expression microarrays and standard in vitro assays were performed on stably silenced WWOX (shRNA) normal breast cells. Bioinformatic analyses were used to identify gene networks and transcriptional regulators affected by WWOX silencing. Co-immunoprecipitations and GST-pulldowns were used to demonstrate a direct interaction between WWOX and SMAD3. Reporter assays, ChIP, confocal microscopy and in silico analyses were employed to determine the effect of WWOX silencing on TGFβ-signaling.

Results
WWOX silencing affected cell proliferation, motility, attachment and deregulated expression of genes involved in cell cycle, motility and DNA damage. Interestingly, we detected an enrichment of targets activated by the SMAD3 transcription factor, including significant upregulation of ANGPTL4, FST, PTHLH and SERPINE1 transcripts. Importantly, we demonstrate that the WWOX protein physically interacts with SMAD3 via WW domain 1. Furthermore, WWOX expression dramatically decreases SMAD3 occupancy at the ANGPTL4 and SERPINE1 promoters and significantly quenches activation of a TGFβ responsive reporter. Additionally, WWOX expression leads to redistribution of SMAD3 from the nuclear to the cytoplasmic compartment. Since the TGFβ target ANGPTL4 plays a key role in lung metastasis development, we performed a meta-analysis of ANGPTL4 expression relative to WWOX in microarray datasets from breast carcinomas. We observed a significant inverse correlation between WWOX and ANGPTL4. Furthermore, the WWOX

lo

/ANGPTL4

hi
cluster of breast tumors is enriched in triple-negative and basal-like sub-types. Tumors with this gene expression signature could represent candidates for anti-TGFβ targeted therapies.

Conclusions
We show for the first time that WWOX modulates SMAD3 signaling in breast cells via direct WW-domain mediated binding and potential cytoplasmic sequestration of SMAD3 protein. Since loss of WWOX expression increases with breast cancer progression and it behaves as an inhibitor of SMAD3 transcriptional activity these observations may help explain, at least in part, the paradoxical pro-tumorigenic effects of TGFβ signaling in advanced breast cancer.
Candida albicans lumbar spondylodiscitis in an intravenous drug user: a case report
Authors: Chen Chang-Hua , Chen Wei Liang , Yen Hua-Cheng 
Published: December 2013

AbstractBackgroundSpondylodiscitis leads to debility, and few data exist onCandidaspondylodiscitis in patients with intravenous drug use.Case presentationWe present a case ofCandida albicanslumbar spondylodiscitis in a patient with intravenous drug use. This patient was treated with surgical debridement and 9 months of fluconazole therapy, and the neurological deficits resolved completely. The infection did not recur clinically or radiologically during 9 months of follow-up.ConclusionAlthoughCandida albicanslumbar spondylodiscitis is rare,Candidashould be suspected as a causative pathogen in patients with intravenous drug use except forStaphylococcus aureus, Pseudomonas aeruginosa,andMycobacterium tuberculosis. As soon asCandida albicanslumbar spondylodiscitis is suspected, magnetic resonance imaging and percutaneous biopsy should be performed. Surgical intervention combined with treatment with antifungal medications can successfully eradicate the infection and resolve the neurological deficits.
Occurrence of potentially pathogenic nontuberculous mycobacteria in Mexican household potable water: a pilot study
Authors: Perez-Martinez Iza , Aguilar-Ayala Diana A , Fernandez-Rendon Elizabeth , Carrillo-Sanchez Alma K , Helguera-Repetto Addy C , Rivera-Gutierrez Sandra , Estrada-Garcia Teresa , Cerna-Cortes Jorge F , Gonzalez-y-Merchand Jorge A 
Published: December 2013

Abstract

Background
Nontuberculous mycobacteria (NTM) are environmental opportunistic pathogens found in natural and human-engineered waters, including drinking water distribution systems and household plumbing. This pilot study examined the frequency of occurrence of NTM in household potable water samples in Mexico City. Potable water samples were collected from the “main house faucet” and kitchen faucet. The presence of aerobic-mesophilic bacteria (AMB), total coliforms (TC), fecal coliforms (FC) and NTM species were determined. Mycobacteria species were identified by PCR restriction enzyme pattern analysis (PRA) of the 65-kDa heat shock protein gene (hsp65) and sequencing of the hypervariable region 2 (V2) of the 16S rRNA gene and of the rpo B gene.

Results
AMB (<100 CFU/ml) were present in 118 out of 120 samples; only two samples were outside guidelines ranges (>100 CFU/ml). TC and FC were detected in four and one samples, respectively. NTM species were recovered from 16% samples (19/120) and included M. mucogenicum (nine), M. porcinum (three), M. avium (three), M. gordonae (one), M. cosmeticum (one), M. fortuitum (one), and Mycobacterium sp (one). All household water samples that contained NTM complied with the standards required to grade the water as “good quality” potable water.

Conclusion
Household potable water may be a potential source of NTM infection in Mexico City.
The cytokine and chemokine expression profile of nucleus pulposus cells: implications for degeneration and regeneration of the intervertebral disc
Authors: Phillips Kate L E , Chiverton Neil , Michael Anthony LR , Cole Ashley A , Breakwell Lee M , Haddock Gail , Bunning Rowena AD , Cross Alison K , Le Maitre Christine L 
Published: December 2013

Abstract
Introduction
The aims of these studies were to identify the cytokine and chemokine expression profile of nucleus pulposus (NP) cells and to determine the relationships between NP cell cytokine and chemokine production and the characteristic tissue changes seen during intervertebral disc (IVD) degeneration.

Methods
Real-time q-PCR cDNA Low Density Array (LDA) was used to investigate the expression of 91 cytokine and chemokine associated genes in NP cells from degenerate human IVDs. Further real-time q-PCR was used to investigate 30 selected cytokine and chemokine associated genes in NP cells from non-degenerate and degenerate IVDs and those from IVDs with immune cell infiltrates (‘infiltrated’). Immunohistochemistry (IHC) was performed for four selected cytokines and chemokines to confirm and localize protein expression in human NP tissue samples.

Results
LDA identified the expression of numerous cytokine and chemokine associated genes including 15 novel cytokines and chemokines. Further q-PCR gene expression studies identified differential expression patterns in NP cells derived from non-degenerate, degenerate and infiltrated IVDs. IHC confirmed NP cells as a source of IL-16, CCL2, CCL7 and CXCL8 and that protein expression of CCL2, CCL7 and CXCL8 increases concordant with histological degenerative tissue changes.

Conclusions
Our data indicates that NP cells are a source of cytokines and chemokines within the IVD and that these expression patterns are altered in IVD pathology. These findings may be important for the correct assessment of the ‘degenerate niche’ prior to autologous or allogeneic cell transplantation for biological therapy of the degenerate IVD.
Porcine adipose-derived stem cells from buccal fat pad and subcutaneous adipose tissue for future preclinical studies in oral surgery
Authors: Niada Stefania , Ferreira Lorena Maria , Arrigoni Elena , Addis Alessandro , Campagnol Marino , Broccaioli Eugenio , Brini Anna Teresa 
Published: December 2013

Abstract
Introduction
Adipose-derived stem cells (ASCs) are progenitor cells used in bone tissue engineering and regenerative medicine. Despite subcutaneous adipose tissue being more abundant, the buccal fat pad (BFP) is easily accessible for dentists and maxillofacial surgeons. For this reason, considering the need for preclinical study and the swine as an optimal animal model in tissue engineering applications, we compared the features of porcine ASCs (pASCs) from both tissue-harvesting sites.

Methods
ASCs were isolated from interscapular subcutaneous adipose tissue (ScI) and buccal fat pads of six swine. Cells were characterized for their stemness and multipotent features. Moreover, their osteogenic ability when cultured on titanium disks and silicon carbide-plasma-enhanced chemical vapor-deposition fragments, and their growth in the presence of autologous and heterologous serum were also assessed.

Results
Independent of the harvesting site, no differences in proliferation, viability, and clonogenicity were observed among all the pASC populations. Furthermore, when induced toward osteogenic differentiation, both ScI- and BFP-pASCs showed an increase of collagen and calcified extracellular matrix (ECM) production, alkaline phosphatase activity, and osteonectin expression, indicating their ability to differentiate toward osteoblast-like cells. In addition, they differentiated toward adipocyte-like cells, and chondrogenic induced pASCs were able to increase glycosaminoglycans (GAGs) production over time. When cells were osteoinduced on synthetic biomaterials, they significantly increased the amount of calcified ECM compared with control cells; moreover, titanium showed the osteoinductive effect on pASCs, also without chemical stimuli. Finally, these cells grew nicely in 10% FBS, and no benefits were produced by substitution with swine serum.

Conclusions
Swine buccal fat pad contains progenitor cells with mesenchymal features, and they also osteo-differentiate nicely in association with synthetic supports. We suggest that porcine BFP-ASCs may be applied in preclinical studies of periodontal and bone-defect regeneration.
Choice alters Drosophila oviposition site preference on menthol
Authors: Abed-Vieillard Dehbia , Cortot Jérôme , Everaerts Claude , Ferveur Jean-François 
Published: December 2013

Summary
Food choice and preference relies on multiple sensory systems that are under the control of genes and sensory experience. Exposure to specific nutrients and nutrient-related molecules can change food preference in vertebrates and invertebrates. For example, larval exposure of several holometabolous insects to menthol can change their adult response to this molecule. However, studies involving Drosophila melanogaster exposure to menthol produced controversial results due maybe to methodological differences. Here, we compared the oviposition-site preference of wild-type D. melanogaster lines freely or forcibly exposed to menthol-rich food. After 12 generations, oviposition-site preference diverged between the two lines. Counterintuitively, menthol ‘forced’ lines showed a persistent aversion to menthol whereas ‘free choice’ lines exhibited a decreased aversion to menthol-rich food. This effect was specific to menthol since the ‘free choice’ lines showed unaltered responses to caffeine and sucrose. This suggests that the genetic factors underlying Drosophila oviposition site preference are more rapidly influenced when flies have a choice between alternative sources compared to flies permanently exposed to the same aversive substance.
Gain-of-function mutations in the ALS8 causative gene VAPB have detrimental effects on neurons and muscles
Authors: Sanhueza Mario , Zechini Luigi , Gillespie Trudy , Pennetta Giuseppa 
Published: December 2013

Summary
Amyotrophic Lateral Sclerosis (ALS) is a motor neuron degenerative disease characterized by a progressive, and ultimately fatal, muscle paralysis. The human VAMP-Associated Protein B (hVAPB) is the causative gene of ALS type 8. Previous studies have shown that a loss-of-function mechanism is responsible for VAPB-induced ALS. Recently, a novel mutation in hVAPB (V234I) has been identified but its pathogenic potential has not been assessed. We found that neuronal expression of the V234I mutant allele in Drosophila (DVAP-V260I) induces defects in synaptic structure and microtubule architecture that are opposite to those associated with DVAP mutants and transgenic expression of other ALS-linked alleles. Expression of DVAP-V260I also induces aggregate formation, reduced viability, wing postural defects, abnormal locomotion behavior, nuclear abnormalities, neurodegeneration and upregulation of the heat-shock-mediated stress response. Similar, albeit milder, phenotypes are associated with the overexpression of the wild-type protein. These data show that overexpressing the wild-type DVAP is sufficient to induce the disease and that DVAP-V260I is a pathogenic allele with increased wild-type activity. We propose that a combination of gain- and loss-of-function mechanisms is responsible for VAPB-induced ALS.
Silicon-based C–N Cross-coupling Reaction
Authors: Shimizu Kenta , Minami Yasunori , Goto Osamu , Ikehira Hideyuki , Hiyama Tamejiro 
Published: December 2013

Abstract
Palladium-catalyzed C–N bond-forming cross-coupling reaction of N-trimethylsilylamines with aryl bromides and chlorides is found to proceed in the presence of a fluoride activator in 1,3-dimethyl-2-imidazolidinone (DMI), giving triarylamines in excellent yields. When aryl bromide and bis(silyl)amine were used in this reaction, double C–N bond-forming products were obtained in high yields. The present reaction was successfully applied to C–N bond-forming polymerization.
HBIM for Documentation, Dissemination and Management of Built Heritage. The Case Study of St. Maria in Scaria d'Intelvi
Authors: Brumana Raffaella , Georgopoulos Andreas , Oreni Daniela , Raimondi Anna , Bregianni Angeliki 
Published: December 2013

The research presented here is carried out within the INTERREG EU project framework, which aims to the valorisation and dissemination of the role of the Church of St. Maria di Scaria (Como, Italy). It mainly focuses on the Carloni's intervention (XVIII century), a local family of craftsmen, famous across many European cities and regions contributing to the construction of monuments and their rich decoration apparatus. The laser scanning and photogrammetric surveys have been integrated with the on-site stratigraphic analysis and with the scarcely available historical documents, in an attempt to focus on the reconstruction of the main transformations and chronological phases: BIM approach has been experimented as a way of transmitting a piece of the history of the church life to the local people and for tourist purposes. A Historic Building Information Modeling (HBIM) has been developed while investigating the potential of an object library specially generated to illustrate the structural elements, the construction technologies, and the decorative layers, along with the critical aspects faced by standard BIM in a complex geometry shift from surface approach to object modeling. The research contributes to the explanation of the sequence and construction technologies adopted for the vault system, the first two vaults of the nave, with respect to the vault covering the altar and the apse. The HBIM approach development is analysed to help the generation of a vocabulary and an abacus of elements to be geographically referenced across Europe to disseminate typical construction elements and skills.
Primary stability of two uncemented acetabular components of different geometry: hemispherical or peripherally enhanced?
Authors: Antoniades G. , Smith E. J. , Deakin A. H. , Wearing S. C. , Sarungi M. 
Published: December 2013

ObjectiveThis study compared the primary stability of two commercially available acetabular components from the same manufacturer, which differ only in geometry; a hemispherical and a peripherally enhanced design (peripheral self-locking (PSL)). The objective was to determine whether altered geometry resulted in better primary stability.MethodsAcetabular components were seated with 0.8 mm to 2 mm interference fits in reamed polyethylene bone substrate of two different densities (0.22 g/cm3and 0.45 g/cm3). The primary stability of each component design was investigated by measuring the peak failure load during uniaxial pull-out and tangential lever-out tests.ResultsThere was no statistically significant difference in seating force (p = 0.104) or primary stability (pull-out p = 0.171, lever-out p = 0.087) of the two components in the low-density substrate. Similarly, in the high-density substrate, there was no statistically significant difference in the peak pull-out force (p = 0.154) or lever-out moment (p = 0.574) between the designs. However, the PSL component required a significantly higher seating force than the hemispherical cup in the high-density bone analogue (p = 0.006).ConclusionsHigher seating forces associated with the PSL design may result in inadequate seating and increased risk of component malpositioning or acetabular fracture in the intra-operative setting in high-density bone stock. Our results, if translated clinically, suggest that a purely hemispherical geometry may have an advantage over a peripherally enhanced geometry in high density bone stock. Cite this article: Bone Joint Res 2013;2:264–9.
Design management education and work-based learning
Authors: Norman Caroline , Jerrard Robert N. 
Published: December 2013

Abstract
This article explores the high level of design management skills that can be acquired through work-based learning. Design domain work-based learning in business environments is described from the authors’ research and experience in operating work-based learning contracts within a master’s degree in Design Management at Birmingham Institute of Art and Design. The authors’ research was based on a sample of (work-based, learning contract) students’ experiences over five years and involved the analysis of work submitted for assessment, with a view to evidencing the level of acquisition of key design management skills. A quantitative analysis of students’ achievement is described, followed by a qualitative description of work-based learning within their companies. From the research evidence, a core of design management skills was detected within the work-based experience.
Videoscanning (in) an art school community: Pivotal creative tool becomes a medium of collective (self)reflection and curriculum development
Authors: Purg Peter 
Published: December 2013

Abstract
The aim of this mixed-media contribution that includes the article text and an online video, is to interpret the school-cum-online community practice of ‘videoscanning’ as a social as well as creative (eco)system, and discuss it briefly against selected media theory. The first part of the article embeds this explorative practice (of editing short video-reflections on learning events) within the school’s pedagogical setting; to subsequently reveal its potential for curriculum development, as a community-embedded process of research-based innovation; and finally to reflect the practice within selected theoretical coordinates. The multi-layered interplay of theory and practice, between text and video, proves that only positive and creative interactions can secure self-reflection and improvement in social (learning) systems. If designed as communicative (eco)systems, it is especially in pedagogical settings that social media can prove most important – not only as playgrounds and marketplaces of ethical decisions, but also as platforms of aesthetical innovation.
Reviews
Authors: Shreeve Alison , Stevenson Alison , Souleles Nicos 
Published: December 2013

Abstract
Artist, Researcher, Teacher: A Study of Professional Identity in Art and Education, Alan Thornton (2013) Bristol: Intellect, 147 pp., p/bk, ISBN: 9781841506449, £15.95, $25
University Libraries and Space in the Digital World, Graham Matthews and Graham Walton (eds) (2013) UK: Ashgate, 248 pp., h/bk, ISBN 978-1-4094-2382-9, £54, eBook £37
Digital Dieting: From Information Obesity to Intellectual Fitness, Tara Brabazon (2013) Farnham: Ashgate Publishing Limited, pp. 333, ISBN: 9781472409379, h/bk, £35.00
Get-in-the-groove music: Rhythmicking with young children
Authors: Howard Karen , Morford James B. , Campbell Patricia Shehan 
Published: December 2013

Abstract
The expressive capacities of young children in music are understood to develop naturally from infancy, and are home-grown in families where music is valued and present. The act of ‘rhythmicking’, which is engagement in some manner of rhythmic behaviour, is one of children’s expressive features long before they are introduced to musical study in some formal or systematic manner. With a conscious attention to the application of a community music sensibility, an early childhood programme was designed that nurtured children’s playful yet powerful musical engagement, shored up their rhythmicking capacities, and fostered family time for sharing music that was collaboratively made and listened to. Facilitating musicians co-constructed musical experiences with kindergarten children, and efforts were put forward to involve parents and siblings with kindergarten children in an enticing rhythm-driven programme.
Voices from the ‘ghetto’: Music therapy perspectives on disability and music (A response to Joseph Straus’s book Extraordinary Measures: Disability in Music)
Authors: Tsiris Giorgos 
Published: December 2013

Abstract
In 2011 Joseph Straus published the book Extraordinary Measures: Disability in Music. Adopting a sociocultural approach, Straus explores the relationship between disability and music by focusing on how music not only reflects, but also constructs different disability narratives. After providing an overview of the book content and themes, this article focuses on what I perceive as being Straus’s misunderstood guest, that is, music therapy. Writing from my own perspective as a music therapy practitioner-researcher, and in relation to Straus’s characterization of music therapy as a ‘ghetto’, I respond to certain ideas developed in the book and offer some different perspectives regarding music therapy as well as its contribution to the study of disability in music. These perspectives are drawn from practices and theories emerging from music- and culture-centred, as well as resource-oriented and community approaches to music therapy. This response article aims to instigate interdisciplinary exchange and dialogue between different music-related disciplines within the wider field of disability studies.