bluuebunny/crossref_metadata_2025_split huggingface dataset:
High Definition Real-Time Ultrasound (HDLive) of Embryonic and Fetal Malformations before Week 16
Authors: Raga Francisco , Caballero Oscar , Bonilla Francisco , Bonilla-Musoles Fernando , Castillo Juan Carlos , Climent Maria Teresa 
Published: April 2013

ABSTRACT
High definition real-time ultrasound (HDLive US) incorporates software that calculates the propagation of light through surface structures in relation to the light direction.
This light source can be freely positioned in order to illuminate the desired area of interest allowing a better image quality, a better sensation of deepness and easier visualization of fetal surfaces.
Cases of normal embryos, fetuses and common fetal malformations are shown.
The full potential of this new technology is still to be revealed. HDLive represents, in our opinion, an innovative tool and a step toward an even more realistic anatomical visualization of normal and malformed fetuses.

How to cite this article
Bonilla-Musoles F, Raga F, Castillo JC, Bonilla F Jr, Climent MT, Caballero O. High Definition Real- Time Ultrasound (HDLive) of Embryonic and Fetal Malformations before Week 16. Donald School J Ultrasound Obstet Gynecol 2013;7(1):1-8.
Fetal and Maternal Physiology and Ultrasound Diagnosis
Authors: Kadic Aida Salihagic , Predojevic Maja 
Published: April 2013

ABSTRACTFetal developmental potential is determined at the moment of conception by genetic inheritance. However, this development is modulated by environmental factors. It is important to recognize that both, the mother and the fetus, actively participate in the maintenance of the physiological intrauterine environment. Unfortunately, the fetus is not entirely protected from harmful influences of the external factors. By altering the intrauterine environment, these factors can have a long-term effect on fetal health.How to cite this articleKadic AS, Predojevic M, Kurjak A. Fetal and Maternal Physiology and Ultrasound Diagnosis. Donald School J Ultrasound Obstet Gynecol 2013;7(1):9-35.
Early Detection of Fetal Abnormality
Authors: Pooh Ritsuko K 
Published: April 2013

ABSTRACT
After introduction of high-frequency vaginal transducer, transvaginal two-dimensional ultrasound has established a field of sonoembryology and most of the major fetal abnormalities have been detectable in the first trimester. Three-dimensional ultrasound adds an objective and comprehensive information to two-dimensional sonographic findings.

How to cite this article
Pooh RK. Early Detection of Fetal Abnormality. Donald School J Ultrasound Obstet Gynecol 2013;7(1):46-50.
Magnesium Sulfate: Fetal Neuroprotective Role in Reducing the Risk of Cerebral Palsy
Authors: Vladareanu Radu , Constantinescu Simona , Denes Margareta , Chilianu Andrei 
Published: April 2013

ABSTRACT
Advances in perinatal and neonatal medicine have significantly improved survival rates of preterm infants. This improvement has been associated with substantial risk of neurodevelopmental impairments and with increased number of infants with special health care needs. Cerebral palsy is the most well known and potentially most disabling motor abnormality associated with prematurity. There has been limited progress in understanding the causes of cerebral palsy and in developing primary prevention strategies. Several studies have summarized the experimental evidence that supports possible neuroprotective effects of magnesium. Five randomized controlled trials of antenatal magnesium sulfate found a trend of reduced risk of cerebral palsy in preterm infants. Three meta-analyses using the data from these five trials found that magnesium sulfate given to women at risk of premature birth significantly reduced the risk of cerebral palsy without increasing the risk of perinatal or infant death.

How to cite this article
Constantinescu S, Denes M, Chilianu A, Vladareanu R. Magnesium Sulfate: Fetal Neuroprotective Role in Reducing the Risk of Cerebral Palsy. Donald School J Ultrasound Obstet Gynecol 2013;7(1):98-104.
Penile Ossification: a traumatic event or evolutionary throwback? Case report and review of the literature
Authors: Yılmaz Ibrahim Edhem , Barazani Yagil , Tareen Basir 
Published: April 2013

Penile ossification is an exceedingly rare condition, with only a handful of histologically confirmed cases reported in the literature. The most common condition leading to penile ossification is Peyronie’s disease. Other conditions such as gout, ERSD, diabetes mellitus, hyperparathyroidism, and local trauma have also been associated with penile ossification. We report a unique case of near-complete penile ossification of the corporal bodies with histologic confirmation on pathologic review. Our report summarizes the literature regarding this rare entity.
Ureteroiliac fistula secondary to radiotherapy in a patient with single renal metastasis of colon adenocarcinoma
Authors: Dormeus Sarah , Hernández Erick A. , Nicolazzi Mickaël , Barba Javier F. , Algarra Rubén , Tienza Antonio , Pascual Juan I. , Zudaire Juan J. 
Published: April 2013

We report the case of a 61-year-old man diagnosed in 2001 with rectal cancer (stage T3N1M0). The patient was treated by surgery, adjuvant chemotherapy and radiotherapy. In 2009 he was admitted to the urology department with a complaint of right hemiabdominal pain. The anatomopathological investigation reported renal metastasis of colon adenocarcinoma. After surgery he received adjuvant chemotherapy. No tumour recurrence or metastasis were reported at the 22-month follow-up.
Nifedipine-Loaded Polymeric Nanocapsules: Validation of a Stability-Indicating HPLC Method to Evaluate the Drug Entrapment Efficiency and In Vitro Release Profiles
Authors: Granada Andréa , Tagliari Monika Piazzon , Soldi Valdir , Silva Marcos Antônio Segatto , Zanetti-Ramos Betina Ghiel , Fernandes Daniel , Stulzer Hellen Karine 
Published: April 2013

Abstract
A simple stability-indicating analytical method was developed and validated to quantify nifedipine in polymeric nanocapsule suspensions; an in vitro drug release study was then carried out. The analysis was performed using an RP C18 column, UV-Vis detection at 262 nm, and methanol–water (70 + 30, v/v) mobile phase at a flow rate of 1.2 mL/min. The method was validated in terms of specificity, linearity and range, LOQ, accuracy, precision, and robustness. The results obtained were within the acceptable ranges. The nanocapsules, made of poly(ε-caprolactone), were prepared by the solvent displacement technique and showed high entrapment efficiency. The entrapment efficiency was 97.6 and 98.2% for the nifedipine-loaded polymeric nanocapsules prepared from polyvinyl alcohol (PVA) and Pluronic F68 (PF68), respectively. The particle size and zeta potential of nanocapsules were found to be influenced by the nature of the stabilizer used. The mean diameter and zeta potential for nanocapsules with PVA and PF68 were 290.9 and 179.9 nm, and –17.7 mV and –32.7 mV, respectively. The two formulations prepared showed a drug release of up to 70% over 4 days. This behavior indicates the viability of this drug delivery system for use as a controlled-release system.
Development and Validation of a Liquid Chromatography/Mass Spectrometry Method for the Simultaneous Quantitation of Rosuvastatin and Ezetimibe in Human Plasma
Authors: Varghese Susheel John , Thengungal Kochupappy Ravi 
Published: April 2013

Abstract
A simple, rapid, and sensitive LC/electrospray ionization (ESI)-MS method was developed and validated for the simultaneous determination of rosuvastatin (ROS) and ezetimibe (EZE) in human plasma. Following liquid–liquid extraction, the analytes and an internal standard, atorvastatin (ATO), were separated using an isocratic mobile phase comprising 0.1% (v/v) formic acid–methanol (20 + 80, v/v) on an RP-C18 column. Detection was performed on a mass spectrometer by selected ion monitoring using their respective [M-H]– ions, m/z 480 for ROS, m/z 408 for EZE, and m/z 557 for ATO. For both analytes, the method was linear in the range of 0.1 to 10 ng/mL. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. A run time of 4 min made it possible to determine many plasma samples/day. The validated LC/ESI-MS method can be used to study pharmacokinetics, bioavailability, and bioequivalence of combined dosage forms of ROS and EZE.
Cloud Point Extraction and Flame Atomic Absorption Spectrometric Determination of Cadmium and Nickel in Drinking and Wastewater Samples
Authors: Naeemullah  , Kazi Tasneem G , Shah Faheem , Afridi Hassan I , Baig Jameel Ahmed , Soomro Abdul Sattar 
Published: April 2013

Abstract
A simple method for the preconcentration of cadmium (Cd) and nickel (Ni) in drinking and wastewater samples was developed. Cloud point extraction has been used for the preconcentration of both metals, after formation of complexes with 8-hydroxyquinoline (8-HQ) and extraction with the surfactant octylphenoxypolyethoxyethanol (Triton X-114). Dilution of the surfactant-rich phase with acidified ethanol was performed after phase separation, and the Cd and Ni contents were measured by flame atomic absorption spectrometry. The experimental variables, such as pH, amounts of reagents (8-HQ and Triton X-114), temperature, incubation time, and sample volume, were optimized. After optimization of the complexation and extraction conditions, enhancement factors of 80 and 61, with LOD values of 0.22 and 0.52 μg/L, were obtained for Cd and Ni, respectively. The proposed method was applied satisfactorily for the determination of both elements in drinking and wastewater samples.
Simultaneous Determination of Sitagliptin and Metformin in Their Pharmaceutical Formulation
Authors: Rezk Mamdouh R , Riad Safa'a M , Mahmoud Ghada Y , Abdel Aleem Abdel-Aziz El Bayoumi 
Published: April 2013

Abstract
Two simple, accurate, and rapid methods were developed for simultaneous determination of sitagliptin phosphate and metformin hydrochloride in their pharmaceutical formulation. The first is a TLC method coupled with densitometry. The second is an HPLC method using a C18 column. The selectivity of the proposed methods was checked using laboratory-prepared mixtures. The proposed methods were successfully applied to the analysis of sitagliptin phosphate and metformin hydrochloride in bulk form and in their pharmaceutical formulation without interference from other additives.
Development of a Novel Direct Bioautography-Thin-Layer Chromatography Test: Optimization of Growth Conditions for Gram-Positive Bacteria, Bacillus subtilis
Authors: Grzelak Edyta M , Majer-Dziedzic Barbara , Choma Irena M , Pilorz Karol M 
Published: April 2013

Abstract
A TLC-direct bioautography (DB) assay using Bacillus subtilis as test bacteria was developed. Various factors affecting the microorganism's viability on the TLC plates were studied and verified for the flumequine standards. The Dhenasar's method called "direct sample determination" was used for TLC; the antibiotic samples were spotted on the TLC plates and subjected to bioautography without developing with a mobile phase. The best preincubation and incubation times of bacterial broth were found to be 1 h at 37°C and 6 h at 37°C. The optimal viscosity of broth was obtained by the addition of agarose to obtain a 0.05% solution in the Mueller-Hinton broth. The best incubation time of seeded TLC plates was 17 h at 37°C. The plates were visualized by spraying with 0.2% aqueous 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide solution and incubated again for 0.5 h at 37°C. The method was validated by determination of linearity, interday and intraday precision, LOD, and LOQ. The calibration curves showed good linearity in the range 0.005–0.5 μg (0.5–50.0 μg/mL). The regression coefficients were 0.9970 and 0.9955 for intraday and interday plots, respectively. The LOD of flumequine equalled 0.5 μg/mL, i. e., 5 ng of the antibiotic in the spot. The sensitivity of the developed TLC-DB test was compared with that of the two most commonly used standard antimicrobial susceptibility assays: agar disc diffusion and agar cylinder diffusion. The obtained minimum inhibitory concentration values clearly indicate much higher sensitivity of the TLC-DB method compared to the standard antimicrobial susceptibility assays.
Stability Study for 53 Antibiotics in Solution and in Fortified Biological Matrixes by LC/MS/MS
Authors: Gaugain Murielle , Chotard Marie-Pierre , Verdon Eric 
Published: April 2013

Abstract
This study was performed in order to determine the stability of antibiotics belonging to eight families in solution or biological matrix. Knowledge of the stability of antibiotics has to be demonstrated during method development or validation. The stability of stock standard solutions of 53 antibiotics was assessed after determining the appropriate conditions of dissolution and storage. The stability of the same 53 antibiotics after addition to negative control cow milk or pork muscle tissue stored at –18 and –70°C was also assessed. Our concern was to obtain information concerning the stability of antibiotic residues in fortified biological matrixes in order to make easier the implementation of a routine screening method for antibiotic residues within the framework of the French monitoring program. Antibiotic solutions and fortified samples were analyzed using an LC/MS/MS method previously validated for screening purposes and for which it was checked that all pertinent criteria to obtain interpretable stability results were fulfilled. The design for testing the stability of antibiotics in solutions and matrix samples is described, as well as the rules of decision that were observed. Term periods for the stability study ranged from 1 month to 1 year, depending on the class of compounds. The results presented in this article will be useful and time-saving for many reference and field laboratories because LC/MS/MS methods are more and more commonly used for screening purposes.
Determination of Ochratoxin A in Licorice and Licorice Extracts by High-Performance Liquid Chromatography Coupled with Fluorescence Detection: Collaborative Study
Authors: Lerda Donata , Ambrosio Massimo , Kunsagi Zoltan , Stroka Joerg ,   Collaborators:, Cea J , Cepurnieks G , De Girolamo A , De Rechter P , Gambin D , Ioannou-Kakouri E , Keegan J , Kormali P , Kotello S , Legarda T , Malone B , MacDonald S , Montag V , Motte J-C , M’ller H-T , Nuotio K , Schwadorf K , Thim A-M , Toome M , Vancutsem J 
Published: April 2013

Abstract
A collaborative study was conducted to validate an analytical method for the determination of ochratoxin A (OTA) in licorice (root powder) and licorice extracts (paste and powder). Contents of OTA ranged from 26 to 141 μg/kg and from 8 to 52 μg/kg for licorice extracts and root material, respectively. For the analysis, a test portion is extracted with a mixture of methanol and aqueous sodium bicarbonate solution. The extract is filtered and diluted with phosphate-buffered saline; and OTA is purified with an immunoaffinity column containing antibodies specific to OTA. The purified extract is dried, reconstituted, and quantified by HPLC with fluorescence detection. Twenty laboratories from 13 European Union member states, Uruguay, Turkey, and the United States of America participated in this study. The study was evaluated according to internationally accepted guidelines. The method performance characteristics can be summarized as follows: over a working range of 7.7 to 141 μg/kg OTA, the mean recoveries were 87% for licorice root and 84–88% for licorice extracts; and the RSDs for reproducibility ranged from 10 to 17% and from 11 to 22% in licorice extracts and licorice root, respectively. The method was found to be fit-for-purpose and to fulfill legal requirements as set in EC Regulation No. 401/2006.
RAZOR™ EX Anthrax Air Detection System for Detection of Bacillus anthracis Spores from Aerosol Collection Samples: Collaborative Study
Authors: Hadfield Ted , Ryan Valorie , Spaulding Usha K , Clemens Kristine M , Ota Irene M , Brunelle Sharon L ,   Collaborators:, Beatty S , Durant A , Hilyard E , Jin S , Knight B , Krause M , McDonald N , Mills J , Morrissey J , Roth A , Sander M , Shea A 
Published: April 2013

Abstract
The RAZOR™ EX Anthrax Air Detection System was validated in a collaborative study for the detection of Bacillus anthracis in aerosol collection buffer. Phosphate-buffered saline was charged with 1 mg/mL standardized dust to simulate an authentic aerosol collection sample. The dust-charged buffer was spiked with either B. anthracis Ames at 2000 spores/mL or Bacillus cereus at 20 000 spores/mL. Twelve collaborators participated in the study, with four collaborators at each of three sites. Each collaborator tested 12 replicates of B. anthracis in dust-charged buffer and 12 replicates of B. cereus in dust-charged buffer. All samples sets were randomized and blind-coded. All collaborators produced valid data sets (no collaborators displayed systematic errors) and there was only one invalid data point. After unblinding, the analysis revealed a cross-collaborator probability of detection (CPOD) of 1.00 (144 positive results from 144 replicates, 95% confidence interval 0.975–1.00) for the B. anthracis samples and a CPOD of 0.00 (0 positive results from 143 replicates, 95% confidence interval 0.00–0.0262) for the B. cereus samples. These data meet the requirements of AOAC Standard Method Performance Requirement 2010.003, developed by the Stakeholder Panel on Agent Detection Assays.
Comparative Evaluation of the VIDAS®Listeria monocytogenes Xpress (LMX) for the Detection of Listeria monocytogenes in a Variety of Foods
Authors: Johnson Ronald , Mills John , Pittet Jean-Louis , Hughes Denise 
Published: April 2013

Abstract
The VIDAS® Listeria monocytogenes Xpress (LMX) test is an enzyme-linked fluorescent immunoassay designed for use with the automated VIDAS or mini-VIDAS instruments for the specific detection of L. monocytogenes using a 26 h proprietary enrichment broth. The VIDAS LMX method was validated according to harmonized AOAC Research Institute (RI) and Official Methods of Analysis guidelines in both the AOAC Performance Tested MethodSM (PTM) and GovVal programs. In the PTM comparison studies, the VIDAS LMX method was compared to the U. S. Department of Agriculture-Food Safety and Inspection Service Microbiology Laboratory Guidebook, the U. S. Food and Drug Administration Bacteriological Analytical Manual, and AOAC Official Methods. The comparative food studies consisted of two main parts: internal testing and AOAC independent laboratory testing, which included seven food matrixes (deli ham, processed cheese, vanilla ice cream, cooked shrimp, smoked white fish, frozen spinach, and peanut butter). As part of the AOAC RI GovVal program, the VIDAS LMX method was compared to the Health Canada MFHPB-30 method for the detection of L. monocytogenes in five ready-to-eat (RTE) meats (hot dogs, deli turkey, deli ham, fermented sausage, and liver paté). Twenty replicates of each inoculation level and five uninoculated controls were evaluated in each study. The LMX method also included the use of chromogenic media, chromID™ Ottaviani Agosti agar and chromID™ L. mono. agar, for confirmation of LMX presumptive results. In both the PTM and GovVal evaluations, there were no significant differences in the Chi-square values for the LMX method when compared to reference methods. The additional parameters tested in the PTM evaluation (inclusivity, exclusivity, ruggedness, stability, and lot-to-lot) satisfied the AOAC RI performance requirements. In both the PTM and GovVal validation studies, the VIDAS LMX method demonstrated reliability as a rapid qualitative method for next-day detection of L. monocytogenes in a variety of foods, including RTE meats.
La chanson à succès dans les années 1940. Une modernité culturelle par acclamation?1
Authors: Savoie Chantal 
Published: April 2013

Dans le contexte de travaux plus larges portant sur l’histoire de la chanson à succès par l’analyse des préférences musicales des lectrices du Bulletin des agriculteurs dans les années 1940, nous nous intéressons ici aux éléments qui permettent de comprendre les modalités par lesquelles les goûts musicaux témoignent de l’émergence d’une modernité culturelle au Québec dans les années 1940. Deux postulats orientent notre perspective. Le premier consiste à supposer l’existence d’une modernité culturelle « populaire » qui transite par la forme, les supports et la technologie. Le second sonde la façon dont les thèmes intimes et la focalisation sur l’expression du sentiment amoureux marquent une transformation des valeurs et, surtout, explore les marques formelles et discursives qui portent ces valeurs dans la chanson à succès.
Les relations entre les mouvements étudiants français et québécois au cours des années 1960. Non-ingérence et indifférence1
Authors: Lamarre Jean 
Published: April 2013

Durant les années 1960, à l’instar des gouvernements français et québécois, l’Union nationale des étudiants de France (UNEF), établie en 1907, et l’Union générale des étudiants du Québec (UGEQ), créée en 1964, ont entretenu des relations institutionnelles. L’objectif de cet article est d’en préciser la nature et les effets sur l’orientation du syndicat étudiant québécois. Rappelons que la France a été longtemps perçue par les Québécois – et l’est encore pour certains – comme la référence culturelle, la mère patrie, le pays vers lequel se tourner pour obtenir secours et soutien. L’histoire et la langue communes ont permis aux deux entités, bien que séparées depuis la Conquête britannique, de tisser à partir des années 1960 des relations plus étroites, notamment en matière de culture et d’éducation. Dans le tourbillon étudiant des années 1960, l’UNEF a-t-elle joué le rôle de « grand frère », de modèle pour l’UGEQ ; a-t-elle, par son discours et sa pratique, influencé l’UGEQ ou l’organisation étudiante québécoise ; ou a-t-elle plutôt mené sa contestation de manière autonome ? Pour parvenir à jeter un peu de lumière sur cet aspect méconnu du mouvement étudiant international, nous allons faire état de l’évolution des deux organisations étudiantes, identifier les points de rencontre, les moments de contacts, et analyser leur nature et leur portée afin d’évaluer dans quelle mesure ces relations ont pu ou non influencer leurs parcours respectifs sur le plan du discours et de la pratique. L’UGEQ s’est rapidement positionnée sur la scène internationale afin d’obtenir la crédibilité qu’elle souhaitait. L’union québécoise s’est largement inspirée de l’UNEF en développant sa position internationale et a cherché sans succès un appui tangible de l’UNEF.
Development of a Seedless Floating Growth Process in Solution for Synthesis of Crystalline ZnO Micro/Nanowire Arrays on Graphene: Towards High‐Performance Nanohybrid Ultraviolet Photodetectors
Authors: Liu Jianwei , Lu Rongtao , Xu Guowei , Wu Judy , Thapa Prem , Moore David 
Published: April 2013

AbstractA seedless solution process is developed for controllable growth of crystalline ZnO micro/nanowire arrays directly on single‐layer graphene sheets made in chemical vapor deposition (CVD). In particular, the alignment of the ZnO micro/nanowires correlates well with the density of the wires, which is determined by both the sample configuration in solution and the graphene surface cleaning. With increasing wire density, the ZnO micro/nanowire array alignment may be varied from horizontal to vertical by increasing the physical confinement. Ultraviolet photodetectors based on the vertically aligned ZnO micro/nanowires on graphene show high responsivity of 1.62 A W−1 per volt, a 500% improvement over epitxial ZnO sensors, a 300% improvement over ZnO nanoparticle sensors, and a 40% improvement over the previous best results for nanowire/graphene hybrid sensors. This seedless, floating growth process could be scaled up for large scale growth of oriented ZnO micro/nanowires on graphene at low costs.