bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age-related NADH oxidase (arNOX) activity is significantly reduced in coelomic fluid of long-lived sea urchins
Authors: Talbert Eric , Bodnar Andrea , Morré Dorothy M , Morré D James 
Published: January 2013

Abstract
Age-related NADH oxidase (arNOX) is a cell surface protein shed into the circulation and other body fluids, which generates superoxide. The activity increases with age in human tissues and body fluids (serum, saliva, and perspiration) and is a potential source of age-related oxidative damage. We measured arNOX activity in the coelomic fluid of sea urchin species with different life spans. Coelomic fluid of long-lived sea urchin species Strongylocentrotus purpuratus and Strongylocentrotus franciscanus exhibited low levels of arNOX compared to the short-lived urchin species Lytechinus variegatus. arNOX activity was positively correlated with animal size in L. variegatus, whereas with S. purpuratus and S. franciscanus, arNOX activity and animal size were inversely correlated. The inverse correlation of arNOX activity with life span and decreased levels of arNOX with age in the long-lived species is consistent with a contribution of reduced arNOX activity to slower aging.
Constructing narratives of heroism and villainy: case study of Myriad's BRACAnalysis® compared to Genentech's Herceptin®
Authors: Baldwin A Lane , Cook-Deegan Robert 
Published: January 2013

Abstract
Background
The development of Herceptin® is welcomed as a major advance in breast cancer treatment, while Myriad's development of BRACAnalysis® is a widely used diagnostic. However useful and successful this product is, its presence in the public eye is tainted by predominantly negative press about gene patenting and business practices.

Discussion
While retrospection invites a sharp contrast between Genentech's triumphal narrative of scientific achievement and Myriad's public image as a controversial monopolist, a comparative history of these companies' products reveals two striking consistencies: patents and public discontent. Despite these similarities, time has reduced the narrative to that of hero versus villain: Genentech is lauded - at least for the final outcome of the Herceptin® story - as a corporate good citizen, Myriad as a ruthless mercenary. Since patents undergird both products yet the narratives are so different, the stories raise the question: why have patents taken the fall as the scapegoat in current biotechnology policy debate?

Summary
A widely publicized lawsuit and accompanying bad press have cast Myriad as a villain in the evolving narrative of biotechnology. While the lawsuit suggests that this villainy is attributable to Myriad's intellectual property, we suggest through a comparative case study that, at least in the Myriad case, it is not simply about the patents but also other business strategies the company chose to pursue. Patents were a necessary but not sufficient cause of controversy.
Out of Tlatelolco’s Ruins: Patronage, Devotion, and Natural Disaster at the Shrine of Our Lady of the Angels, 1745–1781
Authors: Ramírez Paul , Taylor William B. 
Published: January 2013

Abstract
Colonial inhabitants of Mexico City were accustomed to coping with natural disasters, including disease epidemics, droughts, floods, and earthquakes, which menaced rich and poor alike and stirred fervent devotion to miraculous images and their shrines. This article revisits the late colonial history of the shrine of Our Lady of the Angels, an image preserved miraculously on an adobe wall in the Indian quarter of Santiago Tlatelolco. The assumption has been that archiepiscopal authorities aiming to deflect public worship toward a more austere, interior spirituality suppressed activities there after 1745 because they saw the devotion as excessively Indian and Baroque. The shrine has served as a barometer of eighteenth-century Bourbon reforms even though its story has not been fully told. This article explores the politics of patronage in the years after the shrine’s closure and in the decades prior to the arrival on the scene of a new Spanish patron in 1776, revealing that Indian caretakers kept the faith well beyond the official intervention, with some help from well-placed Spanish devotees and officials. The efforts of the new patron, a Spanish tailor from the city center, to renovate the building and image and secure the necessary permissions and privileges helped transform the site into one of the most famous in the capital. Attention to earlier patterns of patronage and to the social response to a series of tremors that coincided with his promotional efforts helps to explain why a devotion so carefully managed for enlightened audiences was nevertheless cut from old cloth.
The Regenerated Bone Quality by Implantation of Octacalcium Phosphate Collagen Composites in a Canine Alveolar Cleft Model
Authors: Matsui Aritsune , Matsui Keiko , Handa Takuto , Tanuma Yuji , Miura Kei-Ichiro , Kato Yuta , Kawai Tadashi , Suzuki Osamu , Kamakura Shinji , Echigo Seishi 
Published: January 2013

Objective Synthetic octacalcium phosphate and porcine atelocollagen composites significantly enhanced bone regeneration more than β-tricalcium phosphate collagen composite and hydroxyapatite collagen composite in a rat cranial defect model. However, the long-term stability and quality of octacalcium phosphate collagen (OCP/Col) composites–derived regenerated bone, when implanted in a canine alveolar cleft model, have yet to be elucidated. The present study investigated the longterm stability and quality of bone regenerated by OCP/Col. Design Disks of OCP/Col or collagen were implanted in a canine alveolar-cleft model (n = 6). Then, bone regeneration in the implanted areas was investigated macroscopically, radiographically, and histologically at 10 months after implantation. In addition, three-dimensional quantitative images of regenerated bone were analyzed by microcomputed tomography. Results Macroscopically, the OCP/Col treated alveolus was clearly augmented, and radio-opacity in the OCP/Col implanted area was comparable to that of the original alveolus bone. On histological analysis, the area was mostly filled with newly formed bone, and a few granules of implanted OCP/Col were enclosed in it. In the microcomputed tomography analysis, the regenerated bone volume in the OCP/Col group was larger than that in the collagen group. OCP/Col–derived bone consisted of outer cortical and inner cancellous structure with dense trabeculae and seemed like the original bone structure. Conclusions OCP/Co composites could be a useful bone regenerative material to substitute for autogenous bone because their implantation could elicit high bone regeneration and active structural reconstitution.
On the accuracy of thin-ice thickness retrieval using MODIS thermal imagery over Arctic first-year ice
Authors: Mäkynen Marko , Cheng Bin , Similä Markku 
Published: January 2013

AbstractWe have studied the accuracy of ice thickness (hi) retrieval based on night-time MODIS (Moderate Resolution Imaging Spectroradiometer) ice surface temperature (Ts) images and HIRLAM (High Resolution Limited Area Model) weather forcing data from the Arctic. The study area is the Kara Sea and eastern part of the Barents Sea, and the study period spans November-April 2008–11 with 199 hi charts. For cloud masking of the MODIS data we had to use manual methods in order to improve detection of thin clouds and ice fog. The accuracy analysis of the retrieved hi was conducted with different methods, taking into account the inaccuracy of the HIRLAM weather forcing data. Maximum reliable hi under different air-temperature and wind-speed ranges was 35–50 cm under typical weather conditions (air temperature <–20cC, wind speed <5ms–1) present in the MODIS data. The accuracy is best for the 15–30 cm thickness range, ∼38%. The largest hi uncertainty comes from air temperature data. Our ice-thickness limits are more conservative than those in previous studies where numerical weather prediction model data were not used in the hi retrieval. Our study gives new detailed insight into the capability of Ts-based hi retrieval in the Arctic marginal seas during freeze-up and wintertime, and should also benefit work where MODIS hi charts are used.
Radar-detected englacial stratigraphy in the Pensacola Mountains, Antarctica: implications for recent changes in ice flow and accumulation
Authors: Campbell Seth , Balco Greg , Todd Claire , Conway Howard , Huybers Kathleen , Simmons Christopher , Vermeulen Michael 
Published: January 2013

AbstractWe used measurements of radar-detected stratigraphy, surface ice-flow velocities and accumulation rates to investigate relationships between local valley-glacier and regional ice-sheet dynamics in and around the Schmidt Hills, Pensacola Mountains, Antarctica. Ground-penetrating radar profiles were collected perpendicular to the long axis of the Schmidt Hills and the margin of Foundation Ice Stream (FIS). Within the valley confines, the glacier consists of blue ice, and profiles show internal stratigraphy dipping steeply toward the nunataks and truncated at the present-day ablation surface. Below the valley confines, the blue ice is overlain by firn. Data show that upward-progressing overlap of actively accumulating firn onto valley-glacier ice is slightly less than ice flow out of the valleys over the past ∼1200 years. The apparent slightly negative mass balance (-0.25 cm a-1) suggests that ice-margin elevations in the Schmidt Hills may have lowered over this time period, even without a change in the surface elevation of FIS. Results suggest that (1) mass-balance gradients between local valley glaciers and regional ice sheets should be considered when using local information to estimate regional ice surface elevation changes; and (2) interpretation of shallow ice structures imaged with radar can provide information about local ice elevation changes and stability.
The role of cooperative iceberg capsize in ice-shelf disintegration
Authors: Burton Justin C. , Cathles L. Mac , Wilder W. Grant 
Published: January 2013

AbstractDisintegration of several ice shelves along the Antarctic Peninsula demonstrates a mechanism that involves the conversion of a contiguous ice shelf into an expanding plume of ice-shelf fragments that spreads rapidly across the ocean surface. The growth of surface area and energetic expansion are hypothesized to be driven by gravitational potential energy release associated with iceberg capsize and break-up. Here we investigate this process using a water tank filled with plastic icebergs scaled to represent a laboratory analogue of an expanding plume of ice-shelf fragments (icebergs). Our experiments suggest that hydrodynamic pressure within the water separating neighbouring icebergs is sufficient to couple the motion when their separation is comparable to the iceberg size. This allows one iceberg’s capsize to initiate a ‘domino-like’ effect, where the entire array will subsequently capsize in the same direction and expand across the water surface. Our experimental results motivate the suggestion that cooperative iceberg hydrodynamics is a process that enhances the expansion of ice-shelf fragment plumes during ice-shelf disintegration.
L'ennemie dans l'Europe en guerre au XXe siècle
Authors: Virgili Fabrice 
Published: January 2013

Résumé Dans le contexte guerrier du XX e siècle, les frontières entre civil et militaire, front et arrière, sont de plus en plus brouillées. Dans chaque camp, c’est la société dans sa totalité qui se trouve mobilisée. Penser alors l’ennemi, c’est également le penser « femme ». Trois aspects de cet ennemi au féminin sont ici soulignés. La féminisation de l’ennemi comme moyen de le rejeter dans le camp de l’altérité en retirant à l’autre-combattant sa virilité. L’attitude vis à vis de celle qui sans être encore des ennemies sont pourtant les femmes de l’ennemi (filles, épouses, mères). Qu’il s’agisse de les nier, de les séduire ou de leur faire subir des violences spécifiques (viols). Enfin, lorsque les femmes du camp adverse sont considérées comme de véritables ennemies, qu’elles soient ou non combattantes.
Les horizons de la théorie chez Félibien
Authors: Démoris René 
Published: January 2013

Résumé Cette étude tente d’analyser les positions complexes de Félibien, comme auteur des Entretiens et préfacier rédacteur des Conférences de 1667, quant à la théorie de la peinture, aux divers sens de ce terme, en prenant en considération l’engagement religieux de l’auteur et son refus d’un système totalisant, qui conduit à une dispersion, dans les Entretiens , des textes théoriques. L’affirmation d’un au delà de la théorie assure chez lui une « liberté » de la peinture, dont la signification politique ne doit pas être négligée.
Penser l'enregistrement musical avec Jean-François Lyotard
Authors: Pouradier Maud 
Published: January 2013

Résumé Si Lyotard n’a jamais traité explicitement de l’enregistrement musical, il en propose une pensée en creux dans Des dispositifs pulsionnels et L’inhumain . Contrairement à Adorno ou Benjamin, Lyotard n’accuse pas l’enregistrement de faire de la musique une marchandise comme une autre au sein du système capitaliste. C’est la musique classique occidentale elle-même qui est intrinsèquement mécanique et capitaliste, l’enregistrement ne faisant que renforcer ce processus fondamental, à l’œuvre plus particulièrement dans la « nouvelle musique » de Schoenberg. Mais la loi des séries n’est pas le fin mot de la musique. La plus classique des sonates est toujours postmoderne, en ce qu’un événement sonore inaudible y est présent à travers l’audible, en ce qu’à travers les variations incessantes un thème fondamental toujours absent est présent. Toute musique entendue n’est que l’enregistrement d’un tel événement. Loin de voir dans l’enregistrement musical un accident de la musique, Lyotard pense donc essentiellement la musique comme un enregistrement.
Formalisme et ritualité chez Boulez et Stockhausen
Authors: Dousson Lambert 
Published: January 2013

Résumé De nombreuses musiques cherchent à exercer sur les auditeurs une « efficacité symbolique » : transformer l’instant musical en rite d’initiation, faire entrer les corps en transe, convertir les émotions musicales en émotions corporelles, c’est-à-dire les affects en actions. En s’appuyant sur les écrits théoriques de Pierre Boulez et Karlheinz Stockhausen, cet article tente de montrer comment chez ces deux compositeurs cette utopie d’une subjectivation à travers la ritualisation de l’écoute s’articule à l’entreprise formaliste d’autodéfinition de la musique, en vue de la constituer comme une sphère autonome.
Un hôpital urbain, idéalement implanté dans la ville
Authors: Cantal-Dupart Michel 
Published: January 2013

Résumé Analysons la mémoire urbaine des hôpitaux, c’est-à-dire ses rapports avec la ville. Des « quarantaines » qui rejettent les pestiférés hors la ville aux hygiénistes du XIX e siècle, l’hôpital devient un service urbain central parce que la croissance des villes le rattrape. Aborder sa place dans les territoires ne peut se limiter à une approche statistique. Il est le miroir de sa ville et doit répondre à tous les usagers, patients, soignants et visiteurs. Si le plateau technique hospitalier reste innovant et performant, il doit faire tomber les murs virtuels qui le cernent et l’empêchent d’être au plus près des milieux de vie des patients potentiels.
NFATc1 Induction in Peripheral T and B Lymphocytes
Authors: Hock Matthias , Vaeth Martin , Rudolf Ronald , Patra Amiya Kumar , Pham Duong Anh Thuy , Muhammad Khalid , Pusch Tobias , Bopp Tobias , Schmitt Edgar , Rost Rene , Berberich-Siebelt Friederike , Tyrsin Dimitri , Chuvpilo Sergei , Avots Andris , Serfling Edgar , Klein-Hessling Stefan 
Published: January 2013

Abstract
NFAT transcription factors control the proliferation and survival of peripheral lymphocytes. We have reported previously that the short isoform NFATc1/αA whose generation is induced by immune receptor stimulation supports the proliferation and inhibits the activation-induced cell death of peripheral T and B cells. We will show in this study that in novel bacterial artificial chromosome transgenic mice that express EGFP under the control of entire Nfatc1 locus the Nfatc1/Egfp transgene is expressed as early as in double-negative thymocytes and in nonstimulated peripheral T and B cells. Upon immune receptor stimulation, Nfatc1/Egfp expression is elevated in B, Th1, and Th2 cells, but only weakly in T regulatory, Th9, and Th17 cells in vitro whose generation is affected by TGFβ. In naive lymphocytes, persistent immune receptor signals led to a 3–5 increase in NFATc1/αA RNA levels during primary and secondary stimulation, but a much stronger induction was observed at the protein level. Whereas anti-CD3+CD28 stimulation of primary T cells induces both NFATc1/αA and their proliferation and survival, anti-IgM stimulation of B cells induces NFATc1/αA and proliferation, but activation-induced cell death after 3-d incubation in vitro. The anti-IgM–mediated activation-induced cell death induction of B cells in vitro is suppressed by anti-CD40–, LPS-, and CpG-mediated signals. In addition to inducing NF-κB factors, together with anti-IgM, these signals also support the generation of NFATc1/αA. According to these data and the architecture of its promoter region, the Nfatc1 gene resembles a primary response gene whose induction is affected at the posttranscriptional level.
TRIF-Mediated TLR3 and TLR4 Signaling Is Negatively Regulated by ADAM15
Authors: Ahmed Suaad , Maratha Ashwini , Butt Aisha Qasim , Shevlin Enda , Miggin Sinead M. 
Published: January 2013

Abstract
TLRs are a group of pattern-recognition receptors that play a crucial role in danger recognition and induction of the innate immune response against bacterial and viral infections. The TLR adaptor molecule, Toll/IL-1R domain-containing adaptor inducing IFN (TRIF), facilitates TLR3 and TLR4 signaling and concomitant activation of the transcription factors, NF-κB and IFN regulatory factor 3, leading to proinflammatory cytokine production. Whereas numerous studies have been undertaken toward understanding the role of TRIF in TLR signaling, little is known about the signaling components that regulate TRIF-dependent TLR signaling. To this end, TRIF-interacting partners were identified by immunoprecipitation of the TRIF signaling complex, followed by protein identification using liquid chromatography mass spectrometry. Following stimulation of cells with a TLR3 or TLR4 ligand, we identified a disintegrin and metalloprotease (ADAM)15 as a novel TRIF-interacting partner. Toward the functional characterization of the TRIF:ADAM15 interaction, we show that ADAM15 acts as a negative regulator of TRIF-mediated NF-κB and IFN-β reporter gene activity. Also, suppression of ADAM15 expression enhanced polyriboinosinic polyribocytidylic acid and LPS-mediated proinflammatory cytokine production via TRIF. In addition, suppression of ADAM15 expression enhanced rhinovirus 16 and vesicular stomatitis virus–mediated proinflammatory cytokine production. Interestingly, ADAM15 mediated the proteolytic cleavage of TRIF. Thus, ADAM15 serves to curtail TRIF-dependent TLR3 and TLR4 signaling and, in doing so, protects the host from excessive production of proinflammatory cytokines and matrix metalloproteinases. In conclusion, to our knowledge, our study clearly shows for the first time that ADAM15 plays an unexpected role in TLR signaling, acting as an anti-inflammatory molecule through impairment of TRIF-mediated TLR signaling.
Targeting Macrophage Activation for the Prevention and Treatment of Staphylococcus aureus Biofilm Infections
Authors: Hanke Mark L. , Heim Cortney E. , Angle Amanda , Sanderson Sam D. , Kielian Tammy 
Published: January 2013

Abstract
Biofilm infections often lead to significant morbidity due to their chronicity and recalcitrance to antibiotics. We have demonstrated that methicillin-resistant Staphylococcus aureus (MRSA) biofilms can evade macrophage (MΦ) antibacterial effector mechanisms by skewing MΦs toward an alternatively activated M2 phenotype. To overcome this immune evasion, we have used two complementary approaches. In the first, a proinflammatory milieu was elicited by local administration of classically activated M1 MΦs and in the second by treatment with the C5a receptor (CD88) agonist EP67, which invokes MΦ proinflammatory activity. Early administration of M1-activated MΦs or EP67 significantly attenuated biofilm formation in a mouse model of MRSA catheter-associated infection. Several proinflammatory mediators were significantly elevated in biofilm-infected tissues from MΦ- and EP67-treated animals, revealing effective reprogramming of the biofilm environment to a proinflammatory milieu. A requirement for MΦ proinflammatory activity was demonstrated by the fact that transfer of MyD88-deficient MΦs had minimal impact on biofilm growth. Likewise, neutrophil administration had no effect on biofilm formation. Treatment of established biofilm infections with M1-activated MΦs also significantly reduced catheter-associated biofilm burdens compared with antibiotic treatment. Collectively, these results demonstrate that targeting MΦ proinflammatory activity can overcome the local immune inhibitory environment created during biofilm infections and represents a novel therapeutic strategy.
Addition of Glycosylation to Influenza A Virus Hemagglutinin Modulates Antibody-Mediated Recognition of H1N1 2009 Pandemic Viruses
Authors: Job Emma R. , Deng Yi-Mo , Barfod Kenneth K. , Tate Michelle D. , Caldwell Natalie , Reddiex Scott , Maurer-Stroh Sebastian , Brooks Andrew G. , Reading Patrick C. 
Published: January 2013

AbstractSeasonal influenza A viruses (IAV) originate from pandemic IAV and have undergone changes in antigenic structure, including addition of glycans to the viral hemagglutinin (HA). Glycans on the head of HA promote virus survival by shielding antigenic sites, but highly glycosylated seasonal IAV are inactivated by soluble lectins of the innate immune system. In 2009, human strains of pandemic H1N1 [A(H1N1)pdm] expressed a single glycosylation site (Asn104) on the head of HA. Since then, variants with additional glycosylation sites have been detected, and the location of these sites has been distinct to those of recent seasonal H1N1 strains. We have compared wild-type and reverse-engineered A(H1N1)pdm IAV with differing potential glycosylation sites on HA for sensitivity to collectins and to neutralizing Abs. Addition of a glycan (Asn136) to A(H1N1)pdm HA was associated with resistance to neutralizing Abs but did not increase sensitivity to collectins. Moreover, variants expressing Asn136 showed enhanced growth in A(H1N1)pdm-vaccinated mice, consistent with evasion of Ab-mediated immunity in vivo. Thus, a fine balance exists regarding the optimal pattern of HA glycosylation to facilitate evasion of Ab-mediated immunity while maintaining resistance to lectin-mediated defenses of the innate immune system.