bluuebunny/crossref_metadata_2025_split huggingface dataset:
Chemical Constituents from the Root of Antidesma Pentandrum var. Barbatum
Authors: Chen Yu‐Chang , Cheng Ming‐Jen , Lee Shoiw‐Ju , Tsai Ian‐Lih , Chen Ih‐Sheng 
Published: May 2015

AbstractInvestigation of the root extract of Antidesma pentandrum var. barbatum led to the isolation of seven new compounds, antidesmol (1), antidesmanins E (2) and F (3), antidesnone (4), antidesnol (5), barbatumols A (6) and B (7), together with 14 known compounds including sodium aristolochate‐I (10) and aristolochic acid‐I methyl ester (11).
Three New Cadinene Derivatives from Xenia Puerto‐Galerae
Authors: Shen Ya‐Ching , Tzeng Guo‐Lin , Chen Meng‐Hsien , Khalil Ashraf Taha 
Published: May 2015

AbstractChromatographic investigation of an acetone extract of the octocoral Xenia puerto‐galerae afforded three new cadinene sesquiterpenes; 8‐epi‐xenitorin A (1), 10‐epi‐xenitorin C (2), and 7‐isopropenyl‐4,10‐dimethyl‐2,3,4,5‐tetrahydronaphthalene (3), in addition to four known cadinene analogs (4, 11–13) and six xenicanes (5–10). The structures were elucidated through spectroscopic analysis, especially 2D NMR. A biogenetic pathway of 1–3 and analogs was proposed.
Evaluation of the Sorptive Capacity of Sugarcane Bagasse and Its Coal for Heavy Metals in Solution
Authors: Soltan M. E. , Sirry S. M. , Fawzy E. M. 
Published: May 2015

AbstractRecycling of sugarcane bagasse and its coal as metal sorbents to capture metal ions from wastewater is the aim of this study. Thus, stability of sugarcane bagasse and its coal, in addition to the solubilities of metal ions in synthetic solution, were determined in this study at different pH values. Also, sorption of Fe, Mn, Cd, and Pb ions with different concentrations (10‐100 mg L−1) on different grain size fractions of sugarcane bagasse (< 150 > μm) and its coal (< 80 > μm) was carried out under different pH values (2, 4 and 6), dosage (2, 6, and 10 g L−1), time intervals (15‐300 min.) and temperature (20‐50 °C). The results indicated that the sugarcane bagasse and its coal were more stable at pH 6, and the solubilities of metal ions in the synthetic solution exhibited high values at pH 2 more than pH 4 and 6, respectively. Generally, removal of metal ions using the sorbents increased with the decreasing of grain size fractions and with increasing of pH values (6 > 4 > 2), sorbent doses (10 > 6 > 2 g L−1) and initial concentrations of metal ions (10‐100 mg L−1). Coal of sugarcane bagasse was more effective than the sugarcane bagasse for removal of the metal ions from solution. Positive values of ΔH° suggest the endothermic nature of sorption in all cases. The negative Gibb's free energy values indicate the feasibility of the process and spontaneous nature of sorption (Fe‐bagasse coal system), while the positive value of ΔG° suggests the non‐spontaneous character of adsorption of all metals. The negative values of entropy change ΔS° (Pb‐bagasse system) indicate the highly ordered adsorption process in this case, while the positive values of ΔS° show the increased randomness at solid/solution interface during the sorption metal ion on bagasse. The results of activation energy values indicate the order of sorption feasibility is: Pb > Fe > Cd > Mn in the case of bagasse and Fe > Pb > Cd > Mn in the case of coal. Generally, the results of this study suggest that the sugarcane bagasse and its coal might provide an economical method for the removal of metal ions from wastewater.
Purification, Characterization and Physico‐Chemical Properties of Three Galactose‐Specific Lectins from Pumpkin (Cucurbita Maxima) Seed Kernels
Authors: Sarkar Subodh Kumar , Hossain M. Tofazzal , Uddin M. Belal , Absar Nurul 
Published: May 2015

AbstractThree galactose‐specific lectins have been isolated and purified from the extract of pumpkin seed kernels by gel filtration on Sephadex G‐75 with 100% ammonium sulfate saturated crude extract, followed by ion exchange chromatography on DEAE‐cellulose and affinity chromatography on Sepharose 4B. All three lectins were found to be homogeneous as judged by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS‐PAGE). The molecular weights of lectins, PSL‐1, PSL‐2 and PSL‐3, as estimated by gel filtration on Sephadex G‐75 were 40,000, 42,000 and 46,000, and by SDS‐PAGE about 39,500, 41,000 and 45,000, respectively. The lectins, PSL‐1, PSL‐2 & PSL‐3 were dimer in nature and the molecular weights of their subunits were about 25,500 and 14,000; 26,000 and 15,000; and 30,500 and 15,000, respectively. The lectins are glycoproteins with a neutral sugar content of 3‐5%. The lectins agglutinated rat red blood cells and the hemagglutination was inhibited specifically by galactose and galactose‐containing saccharides. The lectins exhibited a strong cytotoxic effect in a brine shrimp lethality bioassay.
A New Biphenylpropanoid from Alpinia Katsumadai
Authors: Huang Wen‐Zhe , Zhang Chao‐Feng , Zhang Mian , Wang Zheng‐Tao 
Published: May 2015

AbstractA new biphenylpropanoid, katsumadin (1), together with seven known ones cardamonin (2), (4E, 6E)‐1,7‐diphenyl‐4,6‐heptadien‐3‐one (3), pinocembrin (4), (4Z, 6E)‐5‐hydroxy‐1,7‐diphenyl‐4, 6‐heptadien‐3‐one (5), 7,4î‐dihydroxy‐5‐methoxyflavanone (6), 3,5‐dihydroxystilbene (7), and alpinetin (8) were isolated from the seeds of Alpinia katsumadai Hayata and identified based on detailed spectral analysis including 2D NMR spectrometry and HRMS. Compounds 1‐4 and 8 showed antiemetic activity.
Synthesis and Reactions of 3‐Methylthiazolo[3,2‐a]Benzimidazole‐2‐Carboxylic Acid Hydrazide: Synthesis of Some New Pyrazole, 1,3‐Thiazoline, 1,2,4‐Triazole and 1,2,4‐Triazolo[3,4‐b]‐1,3,4‐Thiadiazine Derivatives Pendant to Thiazolo[3,2‐a]Benzimidazole Moiety
Authors: Abdel‐Aziz Hatem A. , Hamdy Nehal A. , Farag Ahmad M. , Fakhr Issa M. I. 
Published: May 2015

AbstractThe reaction of 3‐methylthiazolo[3,2‐a]benzimidazole‐2‐carboxylic acid ethyl ester (1) with hydrazine hydrate gives the hydrazide 2 which reacts with CS2/KOH to afford the potassium salt 3. Treatment of 3 with l‐aryl‐2‐bromoethanones 4a,b afforded the 1,3‐thiazoline derivatives 6a,b, respectively, while the reaction of 3 with hydrazine hydrate afforded 1,2,4‐triazole‐3‐thione derivative 9. The reaction of 9 with l‐aryl‐2‐bromoethanones 4a,b and with hydrazonyl chlorides 11a,b gave the 1,2,4‐triazolo[3,4‐b]‐1,3,4‐thiadiazine derivatives 10a,b and 12a,b, respectively. Treatment of hydrazide 2 with phenyl isothiocyanate in refluxing benzene gave the thiosemicarbazide derivative 16. The latter reaction gave 1,3,4‐oxadiazole derivative 17 when benzene was replaced by DMF. Cyclization of the thiosemicarbazide derivative 16 with NaOH resulted in the formation of the 1,2,4‐triazole‐3‐thione derivative 18.
An Environmentally Benign System; A Simple and Effective Procedure for Dehydration of Oximes into Nitriles in Solvent Free Conditions
Authors: Hekmatshoar Rahim , Sajadi Sodeh , Heravi Majid M. 
Published: May 2015

AbstractIn an environmentally benign solvent free system aldoximes are rapidly transformed into nitriles using trimethylsilylchloride under mild conditions.
Interaction of Reactive Brilliant Red with Cetylpyridinium and Application to Determination of Cationic Surfactant in Water
Authors: Zhao He‐Ping , Zhao Xue‐Tao , Bai Jing , Gao Hong‐Wen 
Published: May 2015

AbstractThe microsurface adsorption‐spectral correction technique and light adsorption ratio variation approach were jointly applied to characterization of the interaction of reactive brilliant red (RBR) with cetylpyridinium chloride (CPC) at pH 3.67. The interaction responded with the Langmuir isothermal adsorption. The saturated binding ratio of RBR to CPC is (1.2 ± 0.1): 1, and the bind constant of the RBR‐CPC complex was calculated to be (1.15 ± 0.05) × 105 1/mol. The interaction was applied to the determination of cationic surfactant in water by the light adsorption ratio variation approach with satisfactory results.
Selective Adsorption and Molecular Recognition by a Molecularly Imprinted Polymer: Kinetic Study and Analysis
Authors: Li Song‐Jun , Zheng Ming‐Xia , Zhang Jian‐Ming 
Published: May 2015

AbstractThis article presents an original work aiming at kinetically surveying the substrate‐selective mechanism from a molecularly imprinted polymer. With Bisphenol A (BPA) as the template, the imprinted polymer was prepared. The result indicates that the imprinted polymer presents a much more complicated sorption toward the template than toward its analogue. The rate constant in the case of the template appears to be a function of coverage degree. There are also significant deviations from the idealized Langmuir model. Related information indicates that these logically can be a result from the induced molecular memory within the binding framework, which makes the polymer capable of selectively adsorbing the imprint species.
Simultaneous Kinetic‐Potentiometric Determination of Hydrazine and Thiosemicarbazide by Partial Least Squares and Principle Component Regression Methods
Authors: Karimi Mohammad Ali , Abdollahi Hamid , Karami Hassan , Banifatemeh Fatemeh 
Published: May 2015

AbstractSimultaneous determination of hydrazine (HZ) and thiosemicarbazide (TSC) by partial least squares (PLS) and principle component regression (PCR) was carried out based on kinetic data of novel potentiometry. The rate of chloride ion production in reaction of HZ and TSC with N‐chlorosuccinimide (NCS) was monitored by a chloride ion‐selective electrode. The experimental dada shows not only the good ability of ion‐selective electrodes (ISEs) as detectors for the direct determination of chloride ions but also for simultaneous kinetic‐potentiometric analysis using chemometrics methods. The methods are based on the difference observed in the production rate of chloride ions. The results show that simultaneous determination of HZ and TSC can be performed in their concentration ranges of 0.7‐20.0 and 0.5‐20.0 μg mL−1, respectively. The total relative standard error for applying PLS and PCR methods to 9 synthetic samples in the concentration ranges of 0.8‐10 μg mL−1 of TSC and 1.0‐12.0 μg mL−1 of HZ was 4.62 and 4.98, respectively. The effects of certain foreign ions upon the reaction rate were determined for the assessment of the selectivity of the method. Both methods (PLS and PCR) were validated using a set of synthetic sample mixtures and then applied for simultaneous determination of HZ and TSC in water samples.
Synthesis and NMR Study of α‐Keto Stabilized Ylides of the Type RCOCH=PAr3, (R=MeO‐C6H4, Cl‐C6H4, NO2‐C6H4, α‐Thiophenyl and Naphthyl; Ar = p‐Tolyl or Phenyl)
Authors: Sabounchei Seyyed Javad , Nemattalab Hassan , Jodaian Vida 
Published: May 2015

AbstractSynthesis and characterization of five new phosphonium salts and related phosphorus ylides of the type R‐COCH=PAr3 are reported. The reaction of bromo 4′‐methoxo/chloro/nitro acetophenone, α‐thiophenyl and naphthoyl acetophenone with triphenylphosphine or triparatolylphosphine in acetone gives phosphonium salts. Dehydrogenation of these salts in aqueous sodium hydroxide solution form ylides. Characterization of obtained compounds was performed by IR, 1H, 13C, 31PNMR and elemental analysis.
Oxidation of 3‐Furfurylcarbinols with Bromine in Acetone‐Water
Authors: Harn Piin‐Jye , Lin Chu‐Chung , Wu Hsien‐Jen 
Published: May 2015

AbstractOxidation of 3‐furfurylcarbinols 3a‐e and 7 with bromine in acetone‐water solution gave the 2‐substituted‐3‐furfurals 4a‐e and 8 in good yields, respectively. Reaction of 2‐alkyl‐3‐furfurylcarbinols 9a and 9b with bromine in acetone‐water gave the bromoalkyl 3‐furfuryl ketones 10a and 10b as the major products. A reaction mechanism via the cis‐trans isomerization of the 2‐ene‐1,4‐diones 13 and 14 was proposed to account for the transposition of the alkyl group of the 3‐furfurylcarbinols 3, 7 and 9 to the 2‐position on the furan ring of the products 4, 8 and 10.
Syntheses and Structural Characterization of 1D Coordination Polymers of Transition‐Metal Atoms Containing 2,4‐Pyridinedicarboxylate Bridges
Authors: Chuang Shu‐Ting , Kuo Ting‐Shen , Shiu Kom‐Bei 
Published: May 2015

AbstractBy employing one bridging ligand, 2,4‐pyridinedicarboxylate (2,4‐pda2−), three one‐dimensional (1D) coordination polymers of [Cu(2,4‐pda)(H2O)2]∞ (1), {[Cu4(2,4‐pda)4(H2O)8]·3H2O}∞ (2), and {[Cd(2,4‐pda)(H2O)3]·H2O}∞ (3) were synthesized. A simple zigzag polymeric chain is observed for both 1 and 3, but a railroad‐like zigzag polymeric chain is observed for 2. Almost all water molecules, whether coordinated or not, are involved in hydrogen bonding interactions and help to tie up the 1D polymeric chains of 1‐3 into a three‐dimensional (3D) network. A comparison about the coordination geometries in all similar coordination polymers available to date is also included.
Synthesis of New Reagent 2,6‐Diacetylpyridine Bis‐4‐Phenyl‐3‐Thiosemicarbazone (2,6‐DAPBPTSC): Selective, Sensitive and Extractive Spectrophotometric Determination of Co(II) in Vegetable, Soil, Pharmaceutical and Alloy Samples
Authors: Reddy S. Adinarayana , Reddy K. Janardhan , Narayana S. Lakshmi , Sarala Y. , Reddy A. Varada 
Published: May 2015

AbstractNew synthesized reagent 2,6‐diacetylpyridine bis‐4‐phenyl‐3‐thiosemicarbazone (2,6‐DAPBPTSC) is proposed as a sensitive and selective analytical reagent for the extractive spectrophotometric determination of cobalt(II). Cobalt(II) forms a reddish brown colored complex with 2,6‐DAPBPTSC, which is extracted into isoamylalcohol, under optimum conditions. The maximum absorption of the isoamylalcohol extract is measured at 400 nm. Beer's law is applied in the range 0.6‐6.0 ppm of cobalt(II). The molar absorptivity and Sandell's sensitivity of the complex is calculated as 2.2 × 104 L mol−1 cm−1 and 2.68 × 10−3 μg cm−2, respectively. An adequate linearity with a correlation coefficient value of 0.969 is obtained for the Co(II)‐2,6‐DAPBPTSC complex. The instability constant of the complex, calculated from Asmus' method is 3.75 × 10−4 The precision and accuracy of the method is checked with calculation of relative standard deviation (n = 5), 0.388 and the detection limit a value is 0.0028 μg mL−1. The method is successfully employed for the determination of cobalt(II) in real samples, such as vegetables, soil, water samples, standard alloy samples, and the performance of the present method was evaluated in terms of Student ‘t’ test and Variance ‘f’ test, which indicates the significance of the present method is an inter comparison of the experimental values, using atomic absorption spectrometer (AAS).
Recent Progress Regarding Regio‐, Site‐, and Stereoselective Formation of Oxetanes in Paternò‐Büchi Reactions
Authors: Abe Manabu 
Published: May 2015

AbstractRecently, the demand for synthetic oxetanes has increased because the highly strained structure allows specific biologic functions in vivo and also serves unique purposes in industrial materials. This review article summarizes recent progress in regio‐, site‐, and stereo‐selective formation of oxetanes via a photochemical process.
Indirect Determination of Sodium Cefotaxime with N‐Propyl Alcohol‐Ammonium Sulfate‐Water System by Extraction‐Flotation of Cuprous Thiocyanate
Authors: Wu Ling‐Li , Zhang Yan , Zhao Wei , Li Quan‐Min 
Published: May 2015

AbstractA novel method for the indirect determination of sodium cefotaxime by the extraction‐flotation of cuprous thiocyanate is described in this paper. The experiment indicated that the degradation of sodium cefotaxime took place in the presence of 0.20 M sodium hydroxide in a boiling water bath for 40 min. At pH 4.0, the thiol group (‐SH) of the degradation product of sodium cefotaxime could reduce Cu(II) to Cu(I) for the formation of the emulsion CuSCN precipitation in the presence of ammonium thiocyanate. By determining the residual amount of Cu(II) in the solution and calculating the flotation yield of CuSCN, the indirect determination of sodium cefotaxime can be achieved. When the concentration of Cu(II) was 5.0 μg mL−1, a good linear relationship was obtained between the flotation yield of CuSCN and the amount of sodium cefotaxime in the range of 0.50∼20 μg mL−1. The linear equation is E = 1.329 + 2.654C with a correlation coefficient r = 0.9988. The detection limit of sodium cefotaxime of this proposed method evaluated by calibration curve (3σ/k) was found to be 0.39 μg mL−1. Every parameter has been optimized and the reaction mechanism has been studied. This method has been successfully applied to the determination of sodium cefotaxime in pharmaceutical formulations, human serum, and urine samples, respectively. Analytical results obtained with this novel method are satisfactory.
On the AMS and EPR Studies of Chinese Cultural Objects
Authors: Yuan Hong‐Chien , Kutschera Walter , Wald Eva Maria , Steier Peter , Li Yong 
Published: May 2015

AbstractAccelerator mass spectrometry (AMS) is a versatile tool in carbon‐14 dating. In the studies of Chinese paintings and calligraphic works on paper substrates, it is necessary to assess the period of preparation. The precision of AMS measurements has been proven to be reliable for estimation, In conformity with historical background of the art works, AMS can be adapted to assess the age, authenticity, artistic information and conservation requirements. The AMS measurement of Chinese ink rubbing on paper, after OxCal data treatment, gave a result of 1105 ± 30BP. During the course of artistic preparations the paper received extensive impact force which induced the formation of entrapped free radicals. Thus, EPR spectrometry was used to investigate the sample, as well as for a print with an age of 345 ± 40BP by AMS dating and a piece of blank paper from 17th century to find out the differences in free radical phenomena.
Chiral Aryl Pyridyl Alcohols as Enantioselective Catalysts in the Addition of Diethylzinc to Substituted Benzaldehydes
Authors: Chang Tien‐Chu , Chen Chinpiao 
Published: May 2015

AbstractChiral (5‐aryl‐10, 10‐dimethyl‐6‐aza‐tricyclo[7.1.1.02,7]undeca‐2(7),3,5‐trien‐8‐yl)‐diphenyl‐methanols were prepared from highly enantiopure (1R)‐(+)‐α‐pinene (> 97% ee), and applied in the enantioselective addition of diethylzinc to substituted benzaldehydes, to yield alcohols with the (S)‐configuration with an enantiomeric excess that typically ranges from 19 to 86%. Importantly, the electron‐withdrawing substituents at the meta‐position of the substituted benzaldehydes exhibited high enantioselectivity during alkylation using diethylzinc.
A Novel One‐Step Synthesis of Symmetrical Dialkyl Trithiocarbonates in the Presence of Phase‐Transfer Catalysis
Authors: Kiasat Ali Reza , Mehrjardi Mehdi Fallah 
Published: May 2015

AbstractSymmetrical trithiocarbonates were directly obtained, in moderate to excellent isolated yields, by the reaction of various primary, secondary, allylic and benzylic halides or alkyl tosylates with a suspension of granulated KOH and alumina in CS2 under phase‐transfer catalysis. In this manner, cyclic trithiocarbonates such as 1,3‐dithiolane‐2‐thione can also be prepared without formation of any polymeric by‐products.
Catalytic Spectrophotometric Determination of Titanium(IV) Using Methylene Blue‐Ascorbic Acid Redox Reaction
Authors: Mousavi H. Zavvar , Pourreza N. 
Published: May 2015

AbstractA highly selective and sensitive catalytic method for the determination of trace amounts of titanium(IV) was developed. The method is based on the catalytic effect of titanium(IV) on the methylene blue‐ascorbic acid redox reaction. The reaction was followed spectrophotometrically by measuring the change in absorbance of methylene blue at 665 nm, 5 minutes after the initiation of the reaction. In this study experimental parameters were optimized and the effect of the presence of various cations and some anions on the determination of titanium(IV) was examined. The calibration graph was linear in the range of 3‐25 ng mL−1 of titanium(IV). The relative standard deviation for the determination of 10 and 20 ng mL−1 of titanium(IV) were 2.64% and 1.51%, respectively (n = 8). The detection limit calculated from three times of standard deviation of blank 3Sb was 0.6 ng mL−1. The method was successfully applied to the determination of titanium(IV) in tap water and ore samples.
Facile Solid‐Phase Organic Synthesis of 5‐Vinyl‐Substituted 1,2,4‐Oxadiazoles from Polymer‐Bound α‐Selenopropionic Acid
Authors: Hu Qiao‐Sheng , Sheng Shou‐Ri , Liu Xiao‐Ling , Hu Fang , Cai Ming‐Zhong 
Published: May 2015

AbstractCyclocondensation of polystyrene‐supported α‐selenopropionic acid with amidoximes in the presence of 1‐(3‐dimethylaminopropyl)‐3‐ethylcarbodiimide hydrochloride (EDC) followed by oxidative deselenation efficiently afforded 5‐vinyl 1,2,4‐oxadiazoles in good yield and purity with a facile work‐up procedure.
An Efficient Synthesis of 3H‐1,5‐Benzodiazepine Derivatives Catalyzed by Heteropolyacids as a Heterogeneous Recyclable Catalyst
Authors: Heravi Majid M. , Sadjadi Samaheh , Oskooie Hossein A. , Hekmatshoar Rahim , Bamoharram Fatemeh F. 
Published: May 2015

Abstract3H‐1,5‐benzodiazepines were synthesized by the condensation of o‐phenylendiamine and various 1,3‐diketones in the presence of various heteropolyacid (HPA) catalysts under mild conditions in very good yields and with high selectivity.
One‐Pot Synthesis of 2‐Arylbenzoxazoles Promoted by Heteropolyacids
Authors: Heravi Majid M. , Sadjadi Samaheh , Oskooie Hossein A. , Shoar Rahim Hekmat , Bamoharram Fatemeh F. 
Published: May 2015

AbstractBenzoxazole derivatives were obtained in high yields with excellent purity from the condensation of 2‐aminophenol with benzaldehydes and benzoic acids in the presence of a catalytic amount of heteropolyacids (HPAs).
Two New Diterpenoids and other Constituents from Isodon Nervosus
Authors: Yan Fu‐Lin , Guo Lan‐Qing , Bai Su‐Ping , Sun Han‐Dong 
Published: May 2015

AbstractTwo new ent‐kaurane diterpenoids, 15β‐hydroxy‐6,7‐seco‐6,11β:6,20‐diepoxy‐1α,7‐olide‐ent‐kaur‐16‐ene (1), 11α,15α‐dihydroxy‐6β‐methoxy‐6,7‐seco‐6,20‐epoxy‐1α,7‐olide‐ent‐kaur‐16‐ene (2), together with four known diterpenoids, nodosin (3), isodocarpin (4), odonicin (5) and maoyecrystal F (6) were isolated from the aerial parts of Isodon nervosus. The structures of the new compounds were elucidated on the basis of their spectral evidence, especially on 2D NMR.
Simultaneous Determination of Atenolol, Rosuvastatin, Spironolactone, Glibenclamide and Naproxen Sodium in Pharmaceutical Formulations and Human Plasma by RP‐HPLC
Authors: Sultana N. , Arayne M. S. , Iftikhar B. 
Published: May 2015

AbstractA rapid and sensitive high‐performance liquid chromatographic method was developed and validated for the simultaneous determination and quantification of atenolol, rosuvastatin, spirnolactone, glibenclamide and naproxen sodium in bulk drugs, pharmaceutical formulations and in human plasma in the presence of internal standard (flurbiprofen). Chromatograms were developed with methanol and water (80:20, v/v) solvent system on a Purospher start, C18 (5 μm, 250 × 4.6 mm) column and pH was adjusted to 3.40 with ortho‐phosphoric acid. Mobile phase was pumped with a flow rate of 0.90 mL/minute with 235 nm UV detection. Standard curves were linear over the concentration range 0.25‐30 μg/mL−1. The coefficients of variation (C.V.%), were < 3% and LOD and LOQ were <0.0154 & 0.06 for inter‐ and intra‐day, respectively. The method was applied to drug interaction studies of atenolol with rosuvastatin, spironolactone, glibenclamide and naproxen to illustrate the scope and application of the methods to manage four different therapeutic classes of drugs, as they are co‐administered.