bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium‐Dependent Lipopeptide Antibiotics against Drug‐Resistant Pathogens Discovered via Host‐Dependent Heterologous Expression of a Cloned Biosynthetic Gene Cluster
Authors: Lai Hung‐En , Woolner V. Helen , Little Rory F. , Woolly Ethan F. , Keyzers Robert A. , Owen Jeremy G. 
Published: August 2024

AbstractHistorically, small molecules biosynthesised by bacteria have been an excellent source for antibacterial drugs. Today, however, the rediscovery of known compounds is a significant hurdle to developing new antimicrobials. Here we use a genome mining and synthetic biology approach to discover the ambocidins: calcium‐dependent lipodepsipeptides that are active against drug‐resistant Gram‐positive pathogens. By cloning a silent biosynthetic gene cluster (the amb cluster) from Streptomyces ambofaciens ATCC 2387 and integrating this into the chromosome of Streptomyces avermitilis we induce expression of ambocidin A and B: two new Nϵ‐hydroxyarginine‐containing cyclic lipodepsipeptides active against drug‐resistant Gram‐positive pathogens. Using a panel of Streptomyces host strains, we show that the choice of heterologous host is critical for producing the biologically active compounds, and that inappropriate host choice leads to aberrant production inactive derivatives. We show that Nϵ‐hydroxyarginine is the product of a heme‐dependent oxygenase and that it enhances biological activity. Ambocidin A inhibits cell wall biosynthesis by binding to Lipid II at a different site than vancomycin. Furthermore, unlike daptomycin, ambocidin A retains potent antimicrobial activity in the presence of lung surfactant, giving it the potential to treat bacterial pneumonia. Our work expands the family of calcium‐dependent lipopeptide antibiotics with a new member exhibiting a distinct mechanism of action.
Organic Cationic‐Coordinated Perfluoropolymer Electrolytes with Strong Li+‐Solvent Interaction for Solid State Li‐Metal Batteries
Authors: Wang Shi , Xiao Shijun , Li Shuanghan , Liu Chao , Cai Henan , Sun Wenqing , Huang Zhen‐Dong , Lai Wen‐Yong 
Published: August 2024

AbstractThe practical application of solid‐state polymer lithium‐metal batteries (LMBs) is plagued by the inferior ionic conductivity of the applied polymer electrolytes (PEs), which is caused by the coupling of ion transport with the motion of polymer segments. Here, solvated molecules based on ionic liquid and lithium salt with strong Li+‐solvent interaction are inserted into an elaborately engineered perfluoropolymer electrolyte via ionic dipole interaction, extensively facilitating Li+ transport and improving mechanical properties. The intensified formation of solvation structures of contact ion pairs and ionic aggregates, as well as the strong electron‐withdrawal properties of the F atoms in perfluoropolymers, give the PE high electrochemical stability and excellent interfacial stability. As a result, Li||Li symmetric cells demonstrate a lifetime of 2500 h and an exceptionally high critical current density above 2.3 mA cm−2, Li||LiFePO4 batteries exhibit consistent cycling for 550 cycles at 10 C, and Li||uncoated LiNi0.8Co0.1Mn0.1O2 cells achieve 1000 cycles at 0.5 C with an average Coulombic efficiency of 98.45 %, one of the best results reported to date based on PEs. Our discovery sheds fresh light on the targeted synergistic regulation of the electro‐chemo‐mechanical properties of PEs to extend the cycle life of LMBs.
Carbon Xerogel as a Novel Minor Conductive Filler for Carbon‐Polymer Composite Bipolar Plates
Authors: Sharma Priyanka , Bilican Abdurrahman , Schmidt Wolfgang , Gutowski Olof , Dippel Ann‐Christin , Matschuk Kimberley , Kopietz Lukas , Weidenthaler Claudia 
Published: August 2024

AbstractThe current research explores the potential of carbon xerogel as a conductive filler in bipolar plates. The composites comprise graphite as the primary conductive filler and polypropylene as the binder. Carbon xerogel is introduced as a minor conductive filler, and its performance is compared with commercial carbon black. Both nanocarbons exhibit resemblances in microstructure, texture, and surface carbon chemistry. Through‐plane conductivity measurements reveal enhanced electrical conductivity upon replacing a fraction of graphite with either nanofiller. Cross‐sectional analyses of the plates employing computed tomography based on X‐ray diffraction and phase contrasts indicate that the observed electrical conductivity difference stems from reduced trapped air during production and the distribution of the minor filler particles. Given the similarities between carbon xerogel and the reference nanofiller, this study introduces the innovative concept of employing carbon xerogel as a filler for conductive bipolar plates.
Unveiling the Therapeutic Potentials of Water Hyacinth (Eichhornia crassipes (Mart.) Solms) Flower against Oxidative Stress, Inflammation and Depressive Disorders: GC‐MS/MS, In Vitro, In Vivo and In Silico Approaches
Authors: Hasnat Hasin , Riti Saima Jahan , Shompa Suriya Akter , Alam Safaet , Islam Md. Mirazul , Kabir Ferdousy , Khan Md. Salim , Shao Chuxiao , Zeng Chunlai , Wang Shuanghu , Geng Peiwu , Al Mamun Abdullah 
Published: August 2024

AbstractWater hyacinth (Eichhornia crassipes (Mart.) Solms) is a highly invasive aquatic weed native to the Amazonia basin, known for its rapid propagation, adaptability, and utilization in traditional medicine. The study aims to unveil the therapeutic potential of water hyacinth flowers methanolic extract (EC CME) and its four kupchan fractions (EC PESF, EC DCMSF, EC EASF, EC ASF) through diversified chemical‐pharmacological approaches. GC‐MS/MS of EC‐CME uncovered a rich tapestry of 72 phytochemical components. In vitro DPPH scavenging assay and total phenolic content determination assay deciphered promising antioxidant assays with remarkably low IC50 values of 0.353 and 0.485 μg/mL, respectively for EC‐ESF and EC‐ASF. Besides, different in vivo tests, including tail emersion, acetic acid‐induced writhing, and thiopental‐induced sleeping test of EC‐CME, yielded a remarkable 8.61±0.29 min of tail immersion time compared to the control's 2.05±0.11 min at the highest dose (600 mg/kg). The best % inhibition of writhing was recorded as 47.96 % accrued in 400 mg/kg dose, indicating robust pain‐relieving properties. The onset and duration of sleep are significantly ameliorated for EC‐CME, unveiling its antidepressant potential. Besides, molecular docking studies along with ADME/T analysis also validated the wet lab findings as well as their safety, efficacy, and drug‐likeliness profile. Finally, this work can be an essential hint for utilizing aquatic weeds in drug development research.
Self‐Assembled BODIPY@Au Core‐Shell Structures for Durable Neuroprotective Phototherapy
Authors: Wu Melody Cai‐Syaun , Wei Jack Hau‐Ting , Fan Ricky Yu‐Syun , Sim Eng Zhi , Yong Ken‐Tye , Gong Tianxun , Kong Kien Voon 
Published: August 2024

AbstractBODIPY analogs are promising photosensitizers for molecular phototherapy; however, they exhibit high dark cytotoxicity and limited singlet oxygen generation capacity. In this study, we developed self‐assembled core‐shell nanophotosensitizers by linking a bipyridine group to BODIPY (Bpy‐BODIPY) and promoting J‐aggregation on gold nanourchins. This design enhances photostability and reduces the energy gap between the lowest singlet excited state and the lower triplet state, facilitating efficient singlet oxygen production. We characterized these nanophotosensitizers using UV‐visible spectroscopy, transmission electron microscopy (TEM), surface‐enhanced Raman spectroscopy (SERS) and dynamic light scattering (DLS), which confirmed the formation of the desired core‐shell structure and J‐aggregates. Notably, Bpy‐BODIPY@Au significantly suppresses tau protein aggregation and enhances neuroprotective action, even in the presence of a phosphatase inhibitor. This work broadens the application of BODIPY chemistry to nanoagents for neuroprotective therapy.
Interfacial chemistry and structural engineering modified carbon fibers for stable sodium metal anodes
Authors: Chu Chenxiao , Wang Chunting , Meng Weisong , Cai Feipeng , Wang Bo , Wang Nana , Yang Jian , Bai Zhongchao 
Published: August 2024

AbstractSodium (Na) metal stands out as a highly promising anode material for high‐energy‐density Na batteries owing to its abundant resources and exceptional theoretical capacity at low redox potential. Nevertheless, the uncontrolled growth of Na dendrites and the accompanying volumetric changes during the plating/stripping process lead to safety concerns and poor electrochemical performances. This study introduces nitrogen and oxygen co‐doped carbon nanofiber networks wrapped carbon felt (NO‐CNCF), serving as Na deposition skeletons to facilitate a highly reversible Na metal anode. The NO‐CNCF framework with uniformly distributed “sodiophilic” functional groups, nanonetwork protuberances, and cross‐linked network scaffold structure can avoid charge accumulation and facilitate the dendrite‐free Na deposition. Benefiting from these features, the NO‐CNCF@Na symmetrical cells demonstrate notable enhancements in cycling stability, achieving 4000 h cycles at 1 mA cm−2 for 1 mAh cm−2 and 2400 h cycles at 2 mA cm−2 for 2 mAh cm−2 with voltage overpotential of approximately 6 and 10 mV, respectively. Furthermore, the NVP//NO‐CNCF@Na full cells achieve stable cycling performance and favorable rate capability. This investigation offers novel insights into fabricating a “sodiophilic” matrix with a multistage structure toward high‐performance Na metal batteries.
Osmapentalenofurans Constructed by Reacting Os≡C1 of Osmapentalyne with Phenols
Authors: Yang Xiaofei , Zhang Kunming , Zhang Yuteng , Liu Hui , Liu Shanting , Fu Debin , Song Jie , Ma Xuexue , Li Ning , Liu Sheng Hua 
Published: August 2024

AbstractOver the past decade, significant research efforts have focused on osmapentalyne, characterized by the more reactive Os≡C7 (Carbon atoms numbered in a clockwise direction on the osmapentalyne skeleton), across areas encompassing electrophilic, nucleophilic, and addition reactions. Nevertheless, the reactivity of osmapentalyne featuring Os≡C1 remains ripe for further exploration. In this investigation, we effectively synthesized a lineage of osmapentalenofurans through the nucleophilic reaction of osmapentalyne incorporating Os≡C1 with phenols. These resulting complexes demonstrate near‐infrared luminescence traits in both solid and liquid states. Particularly noteworthy is the osmapentalenofuran derived from tetraphenylethane (TPE) unit, which showcases remarkable aggregation‐induced emission (AIE) property in the aggregated state. These osmapentalenofurans are also able to further extend their range of reactions, including reactions with base and isonitrile. This study not only broadens the scope of applications for metal aromatics but also furnishes valuable insights into the realm of specialized functional materials.
13C CSA Tensors and Orientational Order of Model and Dimer Mesogens Comprising of Phenyl Benzoate
Authors: Pratap Gallelli , Reddy Yanati Santhosh K. , Lobo Nitin P. , Ramanathan Krishna V. , Narasimhaswamy Tanneru 
Published: August 2024

AbstractA Model mesogen and its symmetrical Dimer made up of phenyl benzoate core unit are investigated by 13C NMR spectroscopy. The existence of layer order in smectic A and smectic C phases of Dimer mesogen is established by powder X‐ray diffraction. The chemical shift anisotropy (CSA) tensors of Model mesogen are determined by 2D separation of undistorted powder patterns by effortless recoupling (SUPER) experiment and are utilized for calculating the order parameters employing the alignment‐induced chemical shifts (AIS). Additionally, 2D separated local field (SLF) NMR is availed for extracting 13C‐1H dipolar couplings for both mesogens and used for computing the order parameters. A good agreement in the order parameters calculated from 13C‐1H dipolar couplings and AIS is observed. Accordingly, the main order parameter (Szz) for the phenyl rings of the Model mesogen is found to be in the range 0.54–0.82, and for the Dimer mesogen, the values span 0.64–0.82 across mesophases. Since the phenyl benzoate core unit is frequently employed structural moiety for constructing the main chain as well as side chain liquid crystalline polymers and liquid crystalline elastomers, the CSA tensors reported here will be of immense utility for the structural characterization of these materials.
Photoinduced Intramolecular C−N Coupling For The Synthesis Of Azolo[1,2,4]Benzothiadiazines Derivatives
Authors: García‐Manzano Eibber J. , Cuellar Micaela A. , Martín Sandra E. , Budén María E. , Barolo Silvia M. 
Published: August 2024

AbstractThis paper explores an original synthesis of novel heterocyclic compounds derived from photoinduced intramolecular coupling reactions, involving the formation of C−N and C−C bonds. Specifically, two classes of compounds were targeted: 4H‐benzo[e]pyrazolo[1,5‐b][1,2,4]thiadiazines and 5‐amino‐6‐thia‐4,5a‐diazaacephenanthrylenes. The synthetic strategy involved nucleophilic substitution reactions to prepare key sulfonamide starting substrates, followed by photoinduced cyclization (via the Substitution Radical Nucleophilic Unimolecular, SRN1) under mild conditions using visible light. The scope of the reaction was explored with different substituent groups on the starting materials, which demonstrated moderate to very good product yields, with 13 examples and yields of up to 78 %. Moreover, this strategy represents the first synthetic approach to obtain 5‐amino‐6‐thia‐4,5a‐diazaphenanthrylenes.
Morphology‐controlled synthesis of multi‐metal‐based spinel oxide nanocatalysts and their performance for oxygen reduction
Authors: Li Can , Pan Jinfong , Chen Xiaobo , Zhang Lihua , Dennett Anna , Bharathan Prabhu , Lee Douglas , Zhou Guangwen , Fang Jiye 
Published: August 2024

AbstractWe present a one‐pot colloidal synthesis method for producing monodisperse multi‐metal (Co, Mn, and Fe) spinel nanocrystals (NCs), including nanocubes, nano‐octahedra, and concave nanocubes. This study explores the mechanism of morphology control, showcasing the pivotal roles of metal precursors and capping ligands in determining the exposed crystal planes on the NC surface. The cubic spinel NCs, terminated with exclusive {100}‐facets, demonstrate superior electrocatalytic activity for the oxygen reduction reaction (ORR) in alkaline media compared to their octahedral and concave cubic counterparts. Specifically, at 0.85 V, (CoMn)Fe2O4 spinel oxide nanocubes achieve a high mass activity of 23.9 A/g and exhibit excellent stability, highlighting the promising ORR performance associated with {100}‐facets of multi‐metal spinel oxides over other low‐index and high‐index facets. Motivated by exploring the correlation between ORR performance and surface atom arrangement (active sites), surface element composition, as well as other factors, this study introduces a prospective approach for shape‐controlled synthesis of advanced spinel oxide NCs. It underscores the significance of catalyst shape control and suggests potential applications as nonprecious metal ORR electrocatalysts.
In vitro antidiabetic and antioxidant activities of Galega officinalis extracts
Authors: Sukhtezari Shokoofeh , Sahari Mohammad Ali , Barzegar Mohsen , Azizi Mohammad Hossain 
Published: August 2024

AbstractThe purpose of the present study was to determine the total phenolic, flavonoid, and galegine content and antioxidant activity, as well as the in vitro antidiabetic potential of different extracts of Galega officinalis using the solvent extraction method. The results demonstrated that the highest yield of extraction (28.05%) and galegine content (17.40 ± 0.04 μg/g of sample) was obtained using water as the solvent (p < .05). However, the highest total phenolic content (TPC) (138.35 ± 0.63 mg GAE per gram of dried GOEs) and total flavonoid content (TFC) (189.12 ± 1.47 mg catechin per gram of dried GOEs) were extracted using A90 (acetone–water, 90:10) solvent. A90 extract exhibited the highest inhibition of sucrase activity (91.42%) (p < .05). Also, the inhibitory activity of A90 against α‐amylase (59.96%), α‐glucosidase (54.3%), and maltase (62.73%) was significantly higher than that of A70 (acetone–water, 70:30) and E20 (ethanol–water, 20:80) (p < .05). According to antioxidant activity results, the highest ABTS•+ (360.5 ± 15.69 μmol Trolox eq per gram of dried GOEs), hydroxyl radical‐scavenging activity (3657.75 ± 21.56 μmol histidine eq per gram of dried GOEs), and FRAP assay (558.18 ± 20.26 μmol FeSO4 eq per gram of dried GOEs) were related to A90, while the best DPPH radical‐scavenging activity and metal‐chelating activity were related to A70 (302.66 ± 2.42 μmol Trolox equivalents per gram of dried GOEs) and E20 (36.5 ± 1.02 μmol EDTA eq per gram of dried GOEs), respectively. Taken together, A90 appears to be the best solvent to get Galega officinalis extract with the highest antioxidant and antidiabetic activity.
The impact of Anatomage table on writing of medical students' case reports: A comparative study
Authors: Kldiashvili Ekaterina , Al‐Rustum Saif , Denekens Josette , van Rossum Herman 
Published: August 2024

AbstractBackground and AimsAcademic performance is essential in medical education, yet comprehensive training programs addressing this need are lacking. Simulation‐based learning has emerged as a valuable tool for developing clinical skills, with the Anatomage table offering a virtual platform to enhance learning experiences. This study aimed to assess the impact of utilizing the Anatomage table on the academic performance and similarity of case reports among seventh‐semester medical students.MethodsA total of 68 students participated, with 25 using paper‐based medical history and 43 using the Anatomage table. Descriptive statistics and Turnitin were employed for data analysis.ResultsStudents using the Anatomage table exhibited higher academic performance and lower similarity percentages compared to those using paper‐based medical history.ConclusionThese findings highlight the benefits of integrating advanced technology into medical education to enhance learning outcomes and prepare students for clinical practice. Further investigation is required to improve the effectiveness of simulation‐based learning in enhancing academic performance of medical students.
B cells critical for outcome in high grade serous ovarian carcinoma
Authors: Vledder Annegé , Paijens Sterre T. , Loiero Dominik , Maagdenberg Alexis , Duiker Evelien W. , Bart Joost , Hendriks Anne M. , Jalving Mathilde , Werner Naomi , van Rooij Nienke , Plat Annechien , Wisman G. Bea A. , Yigit Refika , Roelofsen Thijs , Kruse Arnold J. , de Lange Nastascha M. , Koelzer Viktor H. , de Bruyn Marco , Nijman Hans W. 
Published: August 2024

AbstractRecent work has shown evidence for the prognostic significance of tumor infiltrating B cells (B‐TIL) in high grade serous ovarian carcinoma (HGSOC), the predominant histological subtype of ovarian cancer. However, it remains unknown how the favorable prognosis associated with B‐TIL relates to the current standard treatments of primary debulking surgery (PDS) followed by chemotherapy or (neo‐)adjuvant chemotherapy (NACT) combined with interval debulking surgery. To address this, we analyzed the prognostic impact of B‐TIL in relationship to primary treatment and tumor infiltrating T cell status in a highly homogenous cohort of HGSOC patients. This analysis involved a combined approach utilizing histological data and high‐dimensional flow cytometry analysis. Our findings indicate that while HGSOC tumors pre‐treated with NACT are infiltrated with tumor‐reactive CD8+ and CD4+ TIL subsets, only B‐TIL and IgA plasma blasts confer prognostic benefit in terms of overall survival. Importantly, the prognostic value of B‐TIL and IgA plasma blasts was not restricted to patients treated with NACT, but was also evident in patients treated with PDS. Together, our data point to a critical prognostic role for B‐TIL in HGSOC patients independent of T cell status, suggesting that alternative treatment approaches focused on the activation of B cells should be explored for HGSOC.
A pilot study of upcycled smartphone‐based colposcopy for visual inspection of cervix performed by community healthcare workers in rural Vietnam
Authors: Yim Ga Won , Lee Jiyeon , Yang Kyungmo , Lee Chae Hyeong , Huy Nguyen Vu Quoc , Vo Minh Tuan , Yoon Sangchul 
Published: August 2024

AbstractObjectiveThis study assessed the feasibility of smartphone‐based colposcopy (SBC) for visual inspection of the cervix by community healthcare workers in low‐resource areas.MethodsThis was a retrospective study conducted in community villages in rural Vietnam, where 177 participants were enrolled for a cervical cancer screening. Cervical images were obtained by pre‐trained community healthcare workers using a portable, upcycled SBC (Samsung Galaxy Note 20). Images were taken before and after the visual inspection after acetic acid (VIA) examination. Captured images were stored on a web server through an Android‐based application and later reviewed independently by two experienced gynecologists. Image quality was assessed, and kappa statistics were calculated for the measurement of agreement in VIA findings.ResultsCervical images of 177 women obtained between July and August 2020 were analyzed. The mean age of women was 42 ± 9.1 years, and 20.3% were postmenopausal. The percentage of adequate visibility of the squamocolumnar junction (SCJ) in the captured images was 83.1%. The kappa value for interobserver reliability was 0.61 for VIA positivity agreement between the two gynecologists. Image clarity was rated as average or above in 77.3%. The reasons for suboptimal clarity were poor focusing (15.3%), inadequate SCJ visibility (18%), and obscuring of the transformation zone due to blood (11.3%), discharge (14.7%), or artifacts such as intrauterine devices or polyps (5.1%).ConclusionUpcycled SBC was feasible when performed by pre‐trained healthcare workers in a low‐resource setting. VIA findings by SBC showed adequate agreement between two independent assessments, suggesting its potential as a method to aid cervical cancer screening.
Distant recurrence of non‐muscle invasive bladder cancer 8 years after initial treatment
Authors: Watanabe Mahoro , Kawamorita Naoki , Shiraiwa Tetsuro , Sato Tomonori , Sato Takuma , Kawasaki Yoshihide , Yamashita Shinichi , Ebata Akiko , Sato Satoko , Ito Akihiro 
Published: August 2024

IntroductionDistant recurrence of non‐muscle invasive bladder cancer is a rare condition that is poorly understood and difficult to detect in follow‐up protocols.Case presentationA 73‐year‐old female with a history of T1N0M0 bladder cancer 8 years ago suffered from a left axillary tumor, a left lung tumor, left mediastinal lymph node swelling, and bilateral adrenal gland tumors. Initially, she was diagnosed with metastatic left breast cancer of the left accessory mamma by needle biopsy of an axillary tumor. Subsequent bronchoscopic biopsy of the mediastinal lymph node revealed metastatic urothelial carcinoma, although no recurrence was detected in the urinary tract. She underwent systemic therapy, and all regions shrank without reprogression.ConclusionNon‐muscle invasive bladder cancer should be managed considering distant metastasis. If the origin of the metastatic lesions is unknown, this disease should be considered as a possible origin, even in the absence of urinary tract recurrence.
A comparative study of the tracheary elements of some commercial hardwoods of Nigeria
Authors: Gill L. S. , Onuja J. E. 
Published: August 2024

SummaryA comparative account of tracheary elements of 13 commercial woods of Nigeria is given. The taxa investigated are Canarium schweinfurthii, Diospyros mespiliformis, Scottelia coriacea, Pycnanthus angolense, Lophira alata, Canthium vulgare, Nauclea diderrichii, Fagara leprieurii, Aningeria robusta, Manilkara obovata, Holoptelea grandis, Gmelina arborea and Tectona grandis.