bluuebunny/crossref_metadata_2025_split huggingface dataset:
Na,K-ATPase β-subunit cis homo-oligomerization is necessary for epithelial lumen formation in mammalian cells
Authors: Barwe Sonali P. , Skay Anna , McSpadden Ryan , Huynh Thu P. , Langhans Sigrid A. , Inge Landon J. , Rajasekaran Ayyappan K. 
Published: October 2012

Summary
Na,K-ATPase is a hetero-oligomer of an α- and a β-subunit. The α-subunit (Na,K-α) possesses the catalytic function, whereas the β-subunit (Na,K-β) has cell-cell adhesion function and is localized to the apical junctional complex in polarized epithelial cells. Earlier, we identified two distinct conserved motifs on the Na,K-β1 transmembrane domain that mediate protein-protein interactions: a glycine zipper motif involved in the cis homo-oligomerization of Na,K-β1 and a heptad repeat motif that is involved in the hetero-oligomeric interaction with Na,K-α1. We now provide evidence that knockdown of Na,K-β1 prevents lumen formation and induces activation of extracellular regulated kinases 1 and 2 (ERK1/2) mediated by phosphatidylinositol 3-kinase in MDCK cells grown in three-dimensional collagen cultures. These cells sustained cell proliferation in an ERK1/2-dependent manner and did not show contact inhibition at high cell densities, as revealed by parental MDCK cells. This phenotype could be rescued by wild-type Na,K-β1 or heptad repeat motif mutant of Na,K-β1, but not by the glycine zipper motif mutant that abrogates Na,K-β1 cis homo-oligomerization. These studies suggest that Na,K-β1 cis homo-oligomerization rather than hetero-oligomerization with Na,K-α1 is involved in epithelial lumen formation. The relevance of these findings to pre-neoplastic lumen filling in epithelial cancer is discussed.
ELISA System for Human Endothelial Lipase
Authors: Ishida Tatsuro , Miyashita Kazuya , Shimizu Mamoru , Kinoshita Noriaki , Mori Kenta , Sun Li , Yasuda Tomoyuki , Imamura Shigeyuki , Nakajima Katsuyuki , Stanhope Kimber L , Havel Peter J , Hirata Ken-ichi 
Published: October 2012

BACKGROUNDEndothelial lipase (EL) regulates the metabolism of HDL cholesterol (HDL-C). However, the role of EL in regulating plasma HDL-C concentrations and EL's potential involvement in atherosclerosis in humans has not been fully investigated due to the lack of reliable assays for EL mass. We developed an ELISA system for serum EL mass.METHODSHuman recombinant EL proteins, purified from cultured media of human EL–transfected Chinese hamster ovary cells, were used as antigen and calibrator. Two specific monoclonal antibodies were generated in mice against recombinant EL protein for a sandwich ELISA. We measured EL mass in human serum using EL recombinant protein as a calibration standard.RESULTSThe EL antibodies did not cross-react with lipoprotein lipase and hepatic triglyceride lipase. The detection limit of the ELISA was 20 pg/mL, which is approximately 10 times lower than that of previous ELISA systems. Recovery of spiked EL in serum was 90%–105%. Assay linearity was intact with a &gt;4-fold dilution of serum. Intra- and interassay CVs were &lt;5%. The serum EL mass in 645 human subjects was [mean (SE)] 344.4 (7.7) pg/mL (range 55.2–1387.7 pg/mL). Interestingly, serum EL mass was increased in patients with diagnosed cardiovascular disease and inversely correlated with serum HDL-C concentrations. There was no difference in EL mass between pre- and postheparin plasma samples.CONCLUSIONSThis ELISA should be useful for clarifying the impact of EL on HDL metabolism and EL's potential role in atherosclerosis.
Hilferufe aus dem besetzten Polen
Authors: Löw Andrea 
Published: October 2012

Vorspann
Der große bittere Streit um die Judenräte, den Hannah Arendt 1963 mit ihrem Buch „Eichmann in Jerusalem“ auslöste, zeichnete sich schon 1939/40 ab. Damals waren knapp 1.600 Juden aus Wien in das Generalgouvernement deportiert worden, wo nichts, aber auch gar nichts vorbereitet gewesen war. In ihrer Not und Verzweiflung fühlten sie sich auch von ihrer Heimatgemeinde im Stich gelassen, die harte Worte der Kritik einstecken musste, im Rahmen ihrer Möglichkeiten aber alles tat, um ihren Glaubensbrüdern zu helfen. Andrea Löw, Mitarbeiterin am Institut für Zeitgeschichte München-Berlin und im großen Dokumentationsprojekt „Die Verfolgung und Ermordung der europäischen Juden durch das nationalsozialistische Deutschland 1933-1945“, präsentiert eindrucksvolle Briefe, in denen sich das bedrückende Schicksal dieser Deportierten ebenso spiegelt wie die Ohnmacht der jüdischen Kultusgemeinde Wien.
Paternal Influences on Adolescent Sexual Risk Behaviors: A Structured Literature Review
Authors: Guilamo-Ramos Vincent , Bouris Alida , Lee Jane , McCarthy Katharine , Michael Shannon L. , Pitt-Barnes Seraphine , Dittus Patricia 
Published: October 2012

BACKGROUND AND OBJECTIVE:
To date, most parent-based research has neglected the role of fathers in shaping adolescent sexual behavior and has focused on mothers. The objective of this study was to conduct a structured review to assess the role of paternal influence on adolescent sexual behavior and to assess the methodological quality of the paternal influence literature related to adolescent sexual behavior.

METHODS:
We searched electronic databases: PubMed, PsychINFO, Social Services Abstracts, Family Studies Abstracts, Sociological Abstracts, and the Cumulative Index to Nursing and Allied Health Literature. Studies published between 1980 and 2011 that targeted adolescents 11 to 18 years and focused on paternal parenting processes were included. Methodological quality was assessed by using an 11-item scoring system.

RESULTS:
Thirteen articles were identified and reviewed. Findings suggest paternal factors are independently associated with adolescent sexual behavior relative to maternal factors. The most commonly studied paternal influence was emotional qualities of the father-adolescent relationship. Paternal communication about sex was most consistently associated with adolescent sexual behavior, whereas paternal attitudes about sex was least associated. Methodological limitations include a tendency to rely on cross-sectional design, nonprobability sampling methods, and focus on sexual debut versus broader sexual behavior.

CONCLUSIONS:
Existing research preliminarily suggests fathers influence the sexual behavior of their adolescent children; however, more rigorous research examining diverse facets of paternal influence on adolescent sexual behavior is needed. We provide recommendations for primary care providers and public health practitioners to better incorporate fathers into interventions designed to reduce adolescent sexual risk behavior.
Pediatric Chronic Nonbacterial Osteomyelitis
Authors: Borzutzky Arturo , Stern Sara , Reiff Andreas , Zurakowski David , Steinberg Evan A. , Dedeoglu Fatma , Sundel Robert P. 
Published: October 2012

BACKGROUND AND OBJECTIVES:
Little information is available concerning the natural history and optimal treatment of chronic nonbacterial osteomyelitis (CNO). We conducted a retrospective review to assess the clinical characteristics and treatment responses of a large cohort of pediatric CNO patients.

METHODS:
Children diagnosed with CNO at 3 tertiary care centers in the United States between 1985 and 2009 were identified. Their charts were reviewed, and clinical, laboratory, histopathologic, and radiologic data were extracted.

RESULTS:
Seventy children with CNO (67% female patients) were identified. Median age at onset was 9.6 years (range 3–17), and median follow-up was 1.8 years (range 0–13). Half of the patients had comorbid autoimmune diseases, and 49% had a family history of autoimmunity. Patients with comorbid autoimmune diseases had more bone lesions (P &lt; .001), higher erythrocyte sedimentation rate (P &lt; .05), and higher use of second line therapy (P = .02). Treatment response to nonsteroidal antiinflammatory drugs (NSAIDs), sulfasalazine, methotrexate, tumor necrosis factor α inhibitors, and corticosteroids was evaluated. The only significant predictor of a positive treatment response was the agent used (P &lt; .0001). Estimated probability of response was 57% for NSAIDs, 66% for sulfasalazine, 91% for methotrexate, 91% for tumor necrosis factor α inhibitors, and 95% for corticosteroids.

CONCLUSIONS:
In a US cohort of 70 children with CNO, coexisting autoimmunity was a risk factor for multifocal involvement and treatment with immunosuppressive agents. Disease-modifying antirheumatic drugs and biologics were more likely to lead to clinical improvement than NSAIDs.
Effects of School-Wide Positive Behavioral Interventions and Supports on Child Behavior Problems
Authors: Bradshaw Catherine P. , Waasdorp Tracy E. , Leaf Philip J. 
Published: October 2012

OBJECTIVE:
School-Wide Positive Behavioral Interventions and Supports (SWPBIS) is a universal prevention strategy currently implemented in &gt;16 000 schools across the United States. SWPBIS intends to reduce students’ behavior problems by altering staff behaviors and developing systems and supports to meet children’s behavioral needs. The current study reports intervention effects on child behaviors and adjustment from an effectiveness trial of SWPBIS.

METHODS:
The sample of 12 344 elementary school children was 52.9% male, 45.1% African American, and 46.1% Caucasian. Approximately 49% received free or reduced-priced meals, and 12.9% received special education services at baseline. The trial used a group randomized controlled effectiveness design implemented in 37 elementary schools. Multilevel analyses were conducted on teachers’ ratings of children’s behavior problems, concentration problems, social-emotional functioning, prosocial behavior, office discipline referrals, and suspensions at 5 time points over the course of 4 school years.

RESULTS:
The multilevel results indicated significant effects of SWPBIS on children’s behavior problems, concentration problems, social-emotional functioning, and prosocial behavior. Children in SWPBIS schools also were 33% less likely to receive an office discipline referral than those in the comparison schools. The effects tended to be strongest among children who were first exposed to SWPBIS in kindergarten.

CONCLUSIONS:
These findings provide support for the hypothesized reduction in behavior problems and improvements in prosocial behavior and effective emotion regulation after training in SWPBIS. The SWPBIS framework appears to be a promising approach for reducing problems and promoting adjustment among elementary school children.
Variation in Occult Injury Screening for Children With Suspected Abuse in Selected US Children’s Hospitals
Authors: Wood Joanne N. , Feudtner Chris , Medina Sheyla P. , Luan Xianqun , Localio Russell , Rubin David M. 
Published: October 2012

OBJECTIVE:To describe variation across selected US children’s hospitals in screening for occult fractures in children &lt;2 years old diagnosed with physical abuse and in infants &lt;1 year old who have injuries associated with a high likelihood of physical abuse.METHODS:We performed a retrospective study of children &lt;2 years old with a diagnosis of physical abuse and infants &lt;1 year old with non-motor vehicle crash–associated traumatic brain injuries or femur fractures admitted to 40 hospitals within the Pediatric Hospital Information System database from January 1, 1999, to December 31, 2009. We examined variation among the hospitals in the performance of screening for occult fractures as defined by receipt of skeletal survey or radionuclide bone scan. Marginal standardization implemented with logistic regression analysis was used to examine hospital variation after adjusting for patient demographic characteristics, injury severity, and year of admission.RESULTS:Screening for occult fractures was performed in 83% of the 10 170 children &lt;2 years old with a diagnosis of physical abuse, 68% of the 9942 infants who had a traumatic brain injury, and 77% of the 2975 infants who had femur fractures. After adjustment for patient characteristics, injury severity, and year of admission, hospitals varied significantly in use of screening for occult fractures in all 3 groups of children.CONCLUSIONS:The observed variation in screening for occult fractures in young victims of physical abuse and infants who have injuries associated with a high likelihood of abuse underscores opportunities to improve the quality of care provided to this vulnerable population.
Metformin's Effect on First-Year Weight Gain: A Follow-up Study
Authors: Carlsen Sven M. , Martinussen Marit P. , Vanky Eszter 
Published: October 2012

BACKGROUND:
The impact of metformin medication in pregnant women with polycystic ovary syndrome on weight gain during pregnancy and after delivery and the impact on growth of the offspring are essentially unexplored.

METHODS:
This is a follow-up study of a randomized controlled trial (The Metformin treatment in pregnant PCOS women study), conducted in 11 secondary care centers. Women with PCOS were randomized to metformin (2000 mg daily) or placebo from first trimester to delivery. Questionnaires were sent to 256 participants 1 year postpartum. Maternal weight development in pregnancy and the first year after delivery and offspring anthropometry at birth and weight 1 year postpartum were registered.

RESULTS:
Women randomized to metformin gained less weight during pregnancy compared with those in the placebo group. In the newborns, there was no difference between the 2 groups in weight or length. One year postpartum, women who used metformin in pregnancy lost less weight and their infants were heavier than those in the placebo group (10.2 ± 1.2 kg vs 9.7 ± 1.1 kg, P = .003).

CONCLUSIONS:
Women randomized to metformin were heavier in the first trimester, gained less weight in pregnancy, and lost less weight in the first year postpartum compared with women randomized to placebo. Children exposed to metformin weighed more at 1 year of age.
A New High Strength Drill Pipe Maximizing Safety and Performace in Low Temperature Enviornments
Authors: El Bachiri K. , Machecourt P. , Mauries S. 
Published: October 2012

Abstract
Extreme low temperature and hostile drilling environments such as the "Arctic" are driving the industry to adapt their drilling programs to new challenges and to develop fit-for-purpose drilling string solutions. Proprietary arctic grades already exist for temperatures as low as −40°C. However, for high-strength drill pipe it is necessary to meet the drilling loads associated to temperatures conditions as low as −60°C. Arctic drilling is one of the most vivid examples of how the oil and gas industry is called upon to adapt to ever-increasing demand for energy resources. This evolution is driving the industry to develop suitable drill string solutions. VAM Drilling is at the forefront of these developments for these types of environments.
The need for such drill pipe is derived from well topology constrains in active regions such as Russia, Eastern Europe, CIS, Alaska and Canadian. The underlying issues are not uniquely related to the service temperature of these tubulars but rather to the high risks associated with susceptible damages during ground transportation and surface handling and especially in "arctic" fields (permafrost).
Controlling critical manufacturing parameters is a key factor to reach top of the range products. Steel microstructure, chemical composition, cleanliness and heat treatment process control are critical to achieve high impact toughness on high strength drill pipe. Controlling yield strength and hardness are instrumental to achieve the established product performance and allowing drilling contractors and operators to safety and efficiently drill in these environments.
The paper presents the appropriate steel chemistries and manufacturing controlled processes required to meet safe operating industry standards and performance-driven drilling practices. This includes a range of parameters to be considered by managers and drilling engineers involved with specifications when planning wells and designing drilling products for low temperature environments.
Cementing Abnormally Over-pressurized Formations in Saudi Arabia
Authors: Al-Dossary Abdulla Faleh , Jennings Scott 
Published: October 2012

Abstract
Cementing is one of important and crucial issues in oil field especially for high pressure and gas bearing formations. It is difficult to achieve a good zonal isolation in such formation types where pressure is abnormal and formation fluid contains corrosive fluids and gases. A common problem associated with highly over pressurized zones is cross flow after cementing. Fluid flow from an over pressured zone to a low pressure, high permeability zone can lead to deteriorating the existing production hardware. Work over operations that attempt to repair cement voids including perforation, squeezing and use of casing patches or scab liners are not recommended as they do not provide long lasting results. In one of onshore fields in Saudi Arabia there is a persistent problem related to cementing at high pressure zones. Recently, communication between A (abnormally over pressurized zone) and B (low pressure zone) formations is occurring due to long term sea water injection with increasing frequency, and has resulted in production interruption in several wells. This paper addresses the problems through investigating field practices including drilling, cementing, and completion. It also reviews the field reports and cased hole logs. A three-month study was conducted to evaluate the effects of formation-A water on cement, where the cement was exposed to formation-A water under down hole conditions. The tests for permeability, mechanical properties TGA and EDXRF are presented, in addition to discussions of some of the preliminary findings.
Injectivity Profile Monitoring and Hot Water Flooding Optimization for Horizontal Well 2G on Rospan's Russkoe Heavy Oil Field using DTS
Authors: Rodionov A. , Lachugin D. , Muslimov E. , Makarov E. , Brown G. A. , Nukhaev M. , Tyutikov S. 
Published: October 2012

Abstract
Rospan International, a subsidiary of TNK-BP, is developing its Russkoe heavy-oil field located in the Yamalo-Nenets Autonomous District of Russia. Development of this field commenced with several pilot pads of horizontal wells completed with stand-alone sand-screens because of the unconsolidated sandstone formation. To increase oil recovery hot water flooding through horizontal injectors was employed, however because of high reservoir heterogeneity the injection profile was not uniform and this led to early water breakthrough and an inefficient waterflood, leaving unswept oil reserves. The remote location and harsh environment limited the use of the conventional production logging tools (PLT) for monitoring the injection profile. Because of this, Rospan International chose to install fiber-optic distributed temperature sensing (DTS) system in a ¼-inch control line attached to a stinger hung below the injection tubing along the reservoir section, post-completion, to determine the injection profile and optimize water flooding.
This paper is describes the completion technology used to install the fiber in the horizontal injector and shows the data and results obtained. Numerical thermal models of the near-wellbore environment were used to analyze the shut-in cool-back and hot water velocity injection DTS data to calculate the injectivity profile. Comparison of the inflow profile calculated from the DTS and PLT data proved the value of the installed optic fiber system for injection change monitoring over time and water flooding optimization for Russkoe oilfield.
The First Implementation of Elongated Proppant for Hydraulic Fracturing in Russia
Authors: Kayumov Rifat , Konchenko Andrey , Nevvonen Olga , Firsov Vladislav , Kuznetsov Maxim , Titov Ivan , Kaluder Zdenko 
Published: October 2012

Abstract
The Volga-Urals basin is one of the largest oil-producing regions in Russia. Orenburg region, located in Volga-Urals, has more than 100 oil fields with great variety of formation properties. The majority of formation fluids are characterized by high gas/oil ratio (GOR) and high bubblepoint pressure. Today, most reservoirs are depleted and produce at bottomhole flowing pressure below bubblepoint pressure. These factors yield multiphase and non-Darcy flow in propped hydraulic fractures, which drastically decreases production. As a result, hydraulic fracturing treatments with conventional proppant are not effective.
Proppant flowback is another critical problem after hydraulic fracturing. There are a few solutions widely accepted by industry, and one of the most popular is a resin-coated proppant (RCP). Usually the coating is activated by temperature; however, for formations with low bottomhole static temperature (BHST), RCP may not be efficient. Many formations in Volga-Urals have a low BHST, which requires a different technology from RCP to eliminate proppant flowback.
A recently developed rod-shaped proppant was proposed as a solution to both problems specified above. In comparison with conventional proppant, it provides higher fracture conductivity with integrated flowback control due to random alignment of proppant grains while packing. This property results in improved fracture cleanup from treating fluids. The rod-shaped proppant also acts to prevent proppant flowback through specific shapes of grains. The significant benefit over conventional RCP is that rod-shaped proppant does not have any BHST limitations, does not require any chemical activation, and does not have special flowback requirements.
Since the introduction of rod-shaped proppant in Russia, 10 fracturing treatments have been successfully carried out with this new proppant in Vakhitovskoe, Lebyzhinskoe, Vostochno-Kapitonovskoe, and Sorochinsko-Nikolskoe oil fields. Well production analysis proved that rod-shaped proppant was more effective than conventional proppant: productivity index is 26 to 67% higher. Further, no proppant flowback issues were detected on wells fractured with rod-shaped proppant. The first successful implementation of this product in Russia is described in detail with laboratory data, a thorough production analysis, and case histories.
Determining Reservoir Connectivity and Compositional Grading by Mapping Asphaltene Gradients
Authors: Tsiklakov A. , Weinheber P. , Wichers W. , Zuo J. , Zimin S. , Driller A. , Oshmarin R. 
Published: October 2012

Abstract
Many techniques are used in industry to determine reservoir hydraulic connectivity from static data. These can be rock-based techniques such as seismic mapping, well to well correlations and geological modeling. Or they can be fluid based techniques such as pressure and fluid gradients. Fluid pressure gradients acquired with formation testers have long been popular but they are understood to be able to identify a lack of connectivity and cannot necessarily prove the presence of connectivity.
Recent work has shown that mapping fluid gradients can be much more definitive. For light fluids this mapping is based on the gas-oil ratio (GOR). For heavier fluids, with little GOR variation, this technique requires mapping a different parameter. It has been suspected that asphaltene content was the parameter to map, but until recently the science of asphaltene prediction was unclear. Recent advances in asphaltene science have now clarified the mechanism for asphaltene distribution in the reservoir and gradient prediction is now possible. And most fortunately it turns out that the asphaltene gradient is relatively easy to measure in-situ.
In this paper we present the science behind asphaltene gradient prediction and show how fluid gradients are a superior way to infer reservoir connectivity. We then present data from an Eastern Siberia oilfield where asphaltene gradients are determined in-situ with a wireline formation tester. These gradients are verified by later comparison to laboratory measurements. Finally and most importantly, we show also how the asphaltene content is used to predict reservoir connectivity both vertically and laterally.