bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perbandingan Struktur Skeleton Sirip Ekor Mudskipper Boleophthalmus boddarti dan Periophthalmus chrysospilos
Authors: Hidayah Siti Yuli Nuril , Santoso Hari , Latuconsina Husain 
Published: July 2024

Glodok fish (mudskipper) is a member of the amphibian fish group that uses its tail fin as a tool for movement, both in water and on land. The aim of this study was to compare the skeleton structure and the number of tail fin bones of Boleophthalmus boddarti and Periophthalmus chrysospilos. The sampling location is in Banyuurip Mangrove Center (BMC), Ujungpangkah District, Gresik Regency. The research was carried out in December 2020-January 2021 at the Unisma FMIPA Laboratory. The research method used is descriptive qualitative, with purposive sampling and Alizarin red staining. The results showed that there was no difference in the tail fin skeleton structure of the two species. The modification of the caudal fins of the two species was shown by widening in the second and third spine hemalis and neural spine. The number of caudal fin bones of B. boddarti is the preural centrum os, two epural bones, the parhypural os, five hypural os, and lepidotrichia with two types of fin radius, namely 3 un-segmented and unbranched fin radii and 17 radii segmented and branched. In P. chrysospilos, there are eight cenrum preural os, two epural os, parhypural os, five hypural os, and lepidotrichia with two types of caudal fin radius, namely 3 un-segmented and unbranched fingers and 14 segmented and branched fingers.

Keywords: caudal fin, Boleophthalmus boddarti, Periophthalmus chrysospilos, number of bones
Pengaruh Pendidikan Kesehatan melalui Media Klik KB terhadap Minat Akseptor KB IUD pada Masa Pandemi
Authors: Susiloningtyas Is , Machfudloh Machfudloh , Witantri Adisa Dyah 
Published: July 2024

Pandemi COVID-19 memberikan dampak drop out KB (Keluarga Berencana) dan peningkatan kehamilan. Penggunaan KB IUD (intrauterine device) efektif mengendalikan jumlah penduduk sebesar 99,4%, akan tetapi minat masyarakat terhadap IUD rendah. Minat yang rendah tersebut dapat diatasi dengan pemberian edukasi menggunakan aplikasi klik KB. Penelitian ini bertujuan untuk mengetahui dampak pendidikan kesehatan melalui klik KB terhadap minat akseptor KB IUD pada masa pandemi. Jenis penelitian adalah kuasi eksperimen dengan desain one-group pretest posttest. Penelitian dilakukan bulan Desember – Maret 2022 dengan populasi 311 orang dan sampel yang digunakan 31 orang. Teknik sampling yang digunakan yaitu purposive sampling dengan kriteria inklusi dan eksklusi. Pengambilan data dilakukan dengan penyebaran kuesioner dan pemberian intervensi. Analisis data menggunakan tabel distribusi frekuensi dan uji Wilcoxon. Hasil penelitian menunjukkan mayoritas responden berminat sedang sebelum pemberian pendidikan kesehatan dengan klik KB dan minat responden menjadi tinggi setelah pemberian pendidikan kesehatan dengan klik KB. Pemberian pendidikan kesehatan dengan media aplikasi klik KB berpengaruh terhadap minat akseptor KB IUD di puskesmas wilayah Kabupaten Pemalang dengan nilai p 0,000. Bidan dan Badan Kependudukan dan Keluarga Berencana Nasional (BKKBN) diharapkan bisa melakukan sosialisasi yang lebih menyeluruh kepada masyarakat menggunakan media aplikasi klik KB.
Bone regeneration driven by a nano-hydroxyapatite/chitosan composite bioaerogel for periodontal regeneration
Authors: Souto-Lopes M. , Grenho L. , Manrique Y. , Dias M. M. , Lopes J. C. B. , Fernandes M. H. , Monteiro F. J. , Salgado C. L. 
Published: July 2024

The most recent progress in reconstructive therapy for the management of periodontitis and peri-implantitis bone defects has relied on the development of highly porous biodegradable bioaerogels for guided bone regeneration. The objective of this work was to evaluate in vitro the osteoinduction of periodontal-originating cells (human dental follicle mesenchymal cells, DFMSCs) promoted by a nano-hydroxyapatite/chitosan (nHAp/CS) bioaerogel, which was purified and sterilized by a sustainable technique (supercritical CO2). Moreover, the in vivo bone regeneration capacity of the nHAp/CS bioaerogel was preliminarily assessed as a proof-of-concept on a rat calvaria bone defect model. The quantification of DNA content of DFMSCs seeded upon nHAp/CS and CS scaffolds (control material) showed a significant increase from the 14th to the 21st day of culture. These results were corroborated through confocal laser scanning microscopy analysis (CLSM). Furthermore, the alkaline phosphatase (ALP) activity increased significantly on the 21st day, similarly for both materials. Moreover, the presence of nHAp promoted a significantly higher expression of osteogenic genes after 21 days when compared to CS scaffolds and control. CLSM images of 21 days of culture also showed an increased deposition of OPN over the nHAp/CS surface. The in vivo bone formation was assessed by microCT and histological analysis. The in vivo evaluation showed a significant increase in bone volume in the nHAp/CS test group when compared to CS and the empty control, as well as higher new bone formation and calcium deposition within the nHAp/CS structure. Overall, the present study showed that the nHAp/CS bioaerogel could offer a potential solution for periodontal and peri-implant bone regeneration treatments since the in vitro results demonstrated that it provided favorable conditions for DFMSC proliferation and osteogenic differentiation, while the in vivo outcomes confirmed that it promoted higher bone ingrowth.
Stability of osteotomy in minimally invasive hallux valgus surgery with “8” shaped bandage during gait: a finite element analysis
Authors: Sun Xudong , Guo Ziyan , Cao Xuhan , Xiong Binglang , Pan Yaxin , Sun Weidong , Bai Zixing 
Published: July 2024

IntroductionHallux valgus, a common foot deformity, often necessitates surgical intervention. This study evaluates the biomechanical alterations in patients post-surgery, focusing on the efficacy of an “8” bandage fixation system to promote optimal recovery.MethodsA three-dimensional (3D) model was constructed using CT data from a patient with hallux valgus. A quasi-static finite element analysis (FEA) was conducted in conjunction with gait analysis to evaluate the biomechanical changes at the osteotomy site under “8” shaped bandage fixation following hallux valgus surgery. The effects of the “8” shaped bandage on the stability of the osteotomy site and bone healing were investigated at three load points during the gait cycle.ResultsDuring the Loading Response (LR), Midstance (MSt), and Terminal stance TSt phases, the osteotomy end experienced maximum Von Mises stresses of 0.118, 1.349, and 1.485 MPa, respectively. Correspondingly, the maximum principal stresses, all of which were compressive along the Z-axis, were 0.11662 N, 1.39266 N, and 1.46762 N, respectively. Additionally, these phases showed a maximum relative total displacement of 0.848 mm and a maximum relative shear displacement of 0.872 mm.ConclusionDuring the stance phase, the osteotomy end of the first metatarsal is predominantly subjected to compressive stress, with the relative displacement within the safe range to promote healing. The application of an “8” bandage for external fixation after surgery can maintain the dynamic stability of osteotomy sites post-minimally invasive hallux valgus correction during the gait cycle, thereby promoting the healing of the osteotomy ends.
APH(3’)-Ie, an aminoglycoside-modifying enzyme discovered in a rabbit-derived Citrobacter gillenii isolate
Authors: Lin Naru , Sha Yuning , Zhang Guozhi , Song Chunhan , Zhang Yuan , Zhao Jingxuan , Huang Dawei , Lu Junwan , Bao Qiyu , Pan Wei 
Published: July 2024

BackgroundAminoglycoside-modifying enzymes (AMEs) play an essential role in bacterial resistance to aminoglycoside antimicrobials. With the development of sequencing techniques, more bacterial genomes have been sequenced, which has aided in the discovery of an increasing number of novel resistance mechanisms.MethodsThe bacterial species was identified by 16S rRNA gene homology and average nucleotide identity (ANI) analyses. The minimum inhibitory concentration (MIC) of each antimicrobial was determined by the agar dilution method. The protein was expressed with the pCold I vector in E. coli BL21, and enzyme kinetic parameters were examined. The whole-genome sequence of the bacterium was obtained via the Illumina and PacBio sequencing platforms. Reconstruction of the phylogenetic tree, identification of conserved functional residues, and gene context analysis were performed using the corresponding bioinformatic techniques.ResultsA novel aminoglycoside resistance gene, designated aph(3’)-Ie, which confers resistance to ribostamycin, kanamycin, sisomicin and paromomycin, was identified in the chromosome of the animal bacterium Citrobacter gillenii DW61, which exhibited a multidrug resistance phenotype. APH(3’)-Ie showed the highest amino acid identity of 74.90% with the functionally characterized enzyme APH(3’)-Ia. Enzyme kinetics analysis demonstrated that it had phosphorylation activity toward four aminoglycoside substrates, exhibiting the highest affinity (Km, 4.22 ± 0.88 µM) and the highest catalytic efficiency [kcat/Km, (32.27 ± 8.14) × 104] for ribomycin. Similar to the other APH(3’) proteins, APH(3’)-Ie contained all the conserved functional sites of the APH family. The aph(3’)-Ie homologous genes were present in C. gillenii isolates from different sources, including some of clinical significance.ConclusionIn this work, a novel chromosomal aminoglycoside resistance gene, designated aph(3’)-Ie, conferring resistance to aminoglycoside antimicrobials, was identified in a rabbit isolate C. gillenii DW61. The elucidation of the novel resistance mechanism will aid in the effective treatment of infections caused by pathogens carrying such resistance genes.
Quantitative evaluation of uncertainty and interpretability in machine learning-based landslide susceptibility mapping through feature selection and explainable AI
Authors: Le Xuan-Hien , Choi Chanul , Eu Song , Yeon Minho , Lee Giha 
Published: July 2024

Landslide susceptibility mapping (LSM) is essential for determining risk regions and guiding mitigation strategies. Machine learning (ML) techniques have been broadly utilized, but the uncertainty and interpretability of these models have not been well-studied. This study conducted a comparative analysis and uncertainty assessment of five ML algorithms—Random Forest (RF), Light Gradient-Boosting Machine (LGB), Extreme Gradient Boosting (XGB), K-Nearest Neighbor (KNN), and Support Vector Machine (SVM)—for LSM in Inje area, South Korea. We optimized these models using Bayesian optimization, a method that refines model performance through probabilistic model-based tuning of hyperparameters. The performance of these algorithms was evaluated using accuracy, Kappa score, and F1 score, with accuracy in detecting landslide-prone locations ranging from 0.916 to 0.947. Among them, the tree-based models (RF, LGB, XGB) showed competitive performance and outperformed the other models. Prediction uncertainty was quantified using bootstrapping and Monte Carlo simulation methods, with the latter providing a more consistent estimate across models. Further, the interpretability of ML predictions was analyzed through sensitivity analysis and SHAP values. We also expanded our investigation to include both the inclusion and exclusion of predictors, providing insights into each significant variable through a comprehensive sensitivity analysis. This paper provides insights into the predictive uncertainty and interpretability of ML algorithms for LSM, contributing to future research in South Korea and beyond.
Exploring the molecular landscape of osteosarcoma through PTTG family genes using a detailed multi-level methodology
Authors: Lu Yulin , Wang Danjun , Chen Guoao , Shan Zitong , Li Dongmei 
Published: July 2024

BackgroundOsteosarcoma (OS) poses a significant clinical challenge, necessitating a comprehensive exploration of its molecular underpinnings.MethodsThis study explored the roles of PTTG family genes (PTTG1, PTTG2, and PTTG3P) in OS, employing a multifaceted approach encompassing molecular experiments, including OS cell lines culturing, RT-qPCR, bisulfite and Whole Exome Sequencing (WES) and in silico experiments, including The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets-based validation, overall survival, gene enrichment, functional assays, and molecular docking analyses.ResultsOur findings reveal a consistent up-regulation of PTTG genes in OS cell lines, supported by RT-qPCR experiments and corroborated across various publically available expression datasets databases. Importantly, ROC curve analyses highlight their potential as diagnostic markers. Moving beyond expression profiles, we unveil the epigenetic landscape by demonstrating significant hypomethylation of CpG islands associated with PTTG genes in OS. The negative correlation between methylation status and mRNA expression emphasizes the regulatory role of promoter methylation in PTTG gene expression. Contrary to expectations, genetic mutations in PTTG genes are rare in OS, with only benign mutations observed. Moreover, functional assays also confirmed the oncogenic roles of the PTTG gene in the development of OS. Lastly, we also revealed that Calcitriol is the most appropriate drug that can be utilized to treat OS in the context of PTTG genes.ConclusionThe identification of PTTG genes as potential diagnostic markers and their association with epigenetic alterations opens new avenues for understanding OS pathogenesis and developing targeted therapies. As we navigate the complex landscape of OS, this study contributes essential insights that may pave the way for improved diagnostic and therapeutic strategies in its management.
COVID-19 and menstrual-related disturbances: a Spanish retrospective observational study in formerly menstruating women
Authors: González María , Al-Adib Miriam , Rodríguez Ana B. , Carrasco Cristina 
Published: July 2024

IntroductionFour years after the start of the pandemic, there is limited evidence on the impact of COVID-19 on the women's health regardless of their reproductive status.ObjectiveThe aim was to analyze the prevalence and associated factors of menstrual-related disturbances in formerly menstruating women following SARS-CoV-2 infection.Study designA retrospective observational study of adult women in Spain was conducted during the month of December 2021 using an online survey (N = 17,512). The present analysis includes a subpopulation of SARS-CoV-2-infected and formerly menstruating women (n = 72). The collected data included general characteristics, medical history, and specific information on COVID-19. Chi-square and Mann-Whitney U-tests were performed. Bivariate logistic regression analysis was then performed to investigate possible associations between the occurrence of menstrual-related disturbances after SARS-CoV-2 infection.Results38.8% of participants experienced menstrual-related disturbances following COVID-19. Among these, unexpected vaginal bleeding (20.8%) was the most common event, followed by spotting (11.1%) (
Table 1). Other reported changes were in the length (shorter = 12.5%) and flow (heavier = 30.3%) of menstrual bleeding in comparison to their previous experience. Regression analysis revealed that being a perimenopausal woman [adjusted odds ratio (AOR) 4.721, CI 95%, 1.022–21.796, p = 0.047] and having a previous diagnosis of menorrhagia (AOR 5.824 CI 95%, 1.521–22.310, p = 0.010) were factors associated with the event.ConclusionThese findings could help health professionals provide their patients with up-to-date scientific information to empower them to actively manage their reproductive health, especially in societies where menstrual health is still taboo.
Case report: Managing pemphigus foliaceus using apremilast without systemic glucocorticosteroids or immunosuppressive agents
Authors: Zhang Quanhong , Yu Lang , Wan Li , Chen Liuqing , Chen Jinbo 
Published: July 2024

Pemphigus foliaceus (PF) is a superficial form of pemphigus. Treatment options for PF resemble pemphigus vulgaris, including glucocorticosteroids, immunosuppressive agents and rituximab et al. These treatment approaches can effectively improve the condition but may also be accompanied by high risks of side effects. Therefore, it is crucial to find a safe and effective treatment options for patients with PF. It will not only benefit/be necessary for patients who refuse glucocorticosteroids or immunosuppressive agents treatments, but also for patients who cannot be treated with glucocorticosteroids or immunosuppressive agents. Herein, we reported a case of PF that was treated with apremilast without systemic glucocorticosteroids or immunosuppressive agents. A 54-year-old woman presented with itchy erythema and erosions on the trunk for more than 1 month. The patient applied mometasonefuroate cream without improvement for a duration of two weeks. The past history of diabetes mellitus and atrophic gastritis was reported. Physical examination revealed scattered erythematous macules and erosions on the trunk. No mucosal involvement was observed. The condition was assessed by the pemphigus disease area index and numerical rating scale, with baseline scores of 7 and 8, respectively. Histopathological examination showed acantholysis and intraepithelial blister. Direct immunofluorescence revealed the presence of IgG and Complement 3 deposition between the acanthocytes with the reticular distribution. Based on enzyme-linked immunosorbent assay results, the levels of Dsg1 and Dsg3 antibodies were 28.18 and 0.26 kU/L respectively. The diagnosis of PF was made. This patient was successfully treated with apremilast without systemic glucocorticosteroids or immunosuppressive agents. The patient has continued with apremilast 30mg once daily for maintenance and no adverse events related to apremilast such as gastrointestinal side effects were observed during the 9-month follow-up period. In conclusion, apremilast therapy without systemic glucocorticosteroids nor immunosuppressive agents might provide an effective alternative to management of mild PF without obvious side effect.
Knowledge mapping of severe fever with thrombocytopenia syndrome: a bibliometric analysis
Authors: Zhang Huiying , Zhang Leiliang 
Published: July 2024

BackgroundSevere fever with thrombocytopenia syndrome (SFTS), caused by the Dabie bandavirus (DBV), formerly known as the SFTS virus (SFTSV), is characterized by rapid progression, high morbidity, and mortality. This study aims to analyze the current research status, hotspots, and trends of SFTS since 2009 through bibliometrics, focusing on original research and providing valuable references and inspirations for future basic research, prevention and control of SFTS.MethodsThe Web of Science Core Collection (WOSCC) was used to extract global papers on SFTS from 2009 to 2024. VOSviewer and CiteSpace software were also used to process and visualize results.ResultsA total of 760 publications relevant to SFTS were reviewed. Among these publications, the most active country, author, and publication type included China, Liu Wei, and original articles, respectively. Among the institutions, the National Institute of Infectious Diseases emerged as the top publisher. The most frequently used keywords were “China,” “Bunyavirus,” and “person-to-person transmission.” The bibliometric analysis reviewed and summarized the research results in the field of SFTS and demonstrated the research trends in the field. In addition, the study revealed the current research hotspots and predicted the future research frontiers and potential challenges in the field of SFTS, which will provide references for further exploring and investigating the SFTS-related mechanisms and inspire new therapeutic strategies.ConclusionBibliometric visualization provides an overview of research advances, hotspots, and trends regarding SFTS and consolidates existing knowledge. SFTS research is in a phase of rapid development, and the number of annual publications in the field is growing steadily and rapidly. This is laying the groundwork for further research and providing new ideas for clinicians engaged in SFTS-related therapies and researchers working to improve public health. Currently, researchers are focused on elucidating the biology of SFTS, exploring antibodies, delving into pathogenesis, and investigating specific therapies.
Low muscle mass index is associated with type 2 diabetes risk in a Latin-American population: a cross-sectional study
Authors: Suárez Rosario , Andrade Celina , Bautista-Valarezo Estefania , Sarmiento-Andrade Yoredy , Matos Andri , Jimenez Oliver , Montalvan Martha , Chapela Sebastián 
Published: July 2024

ObjectiveDiabetes mellitus is a growing disease with severe complications. Various scores predict the risk of developing this pathology. The amount of muscle mass is associated with insulin resistance, yet there is no established evidence linking muscle mass with diabetes risk. This work aims to study that relationship.Research methods and proceduresThis cross-sectional study included 1,388 employees. The FINDRISC score was used to assess type 2 diabetes risk, and bioimpedance was used for body composition analysis. Appendicular skeletal muscle mass adjusted by body mass index (ASM/BMI) was analyzed. Sociodemographic, clinical and anthropometric measures were evaluated, logistic regression models with sex stratification were conducted and ROC curves were calculated to determine the ability of ASM/BMI index to predict T2D risk.ResultsIt was observed that patients with higher ASM/BMI had a lower FINDRISC score in both men and women (p &lt; 0.001). A logistic regression model showed and association between ASM/BMI and diabetes risk in women [OR: 0.000 (0.000–0.900), p = 0.048], but not in men [OR: 0.267 (0.038–1.878), p = 0.185]. However, when the body mass index variable was excluded from the model, an association was found between muscle mass adjusted to BMI and diabetes risk in both men [OR: 0.000 (0.000–0.016), p &lt; 0.001], and women [OR:0.001 (0.000–0.034), p &lt; 0.001]. Other risk factors were having a low level of physical activity, waist circumference, age and sedentary lifestyle. A ROC curve was built and the optimal ASM/BMI cut-of value for predicting T2D risk was 0.82 with a sensitivity of 53.71% and specificity of 69.3% [AUC of 0.665 (0.64–0.69; p &lt; 0.0001)].ConclusionWhen quantifying the risk of type 2 diabetes in both women and men, assessing muscle mass can help detect adult individuals with a high risk of developing type 2 diabetes.