bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selection for narrow gate of emergence results in correlated sex-specific changes in life history of Drosophila melanogaster
Authors: Varma Vishwanath , Kannan Nisha N. , Sharma Vijay Kumar 
Published: June 2014

ABSTRACT
Since the ability to time rhythmic behaviours in accordance with cyclic environments is likely to confer adaptive advantage to organisms, the underlying clocks are believed to be selected for stability in timekeeping over evolutionary time scales. Here we report the results of a study aimed at assessing fitness consequences of a long-term laboratory selection for tighter circadian organisation using fruit fly Drosophila melanogaster populations. We selected flies emerging in a narrow window of 1 h in the morning for several generations and assayed their life history traits such as pre-adult development time, survivorship, adult lifespan and lifetime fecundity. We chose flies emerging during the selection window (in the morning) and another window (in the evening) to represent adaptive and non-adaptive phenotypes, respectively, and examined the correlation of emergence time with adult fitness traits. Adult lifespan of males from the selected populations does not differ from the controls, whereas females from the selected populations have significantly shorter lifespan and produce more eggs during their mid-life compared to the controls. Although there is no difference in the lifespan of males of the selected populations, whether they emerge in morning or evening window, morning emerging females live slightly shorter and lay more eggs during the mid-life stage compared to those emerging in the evening. Interestingly, such a time of emergence dependent difference in fitness is not seen in flies from the control populations. These results, therefore, suggest reduced lifespan and enhanced mid-life reproductive output in females selected for narrow gate of emergence, and a sex-dependent genetic correlation between the timing of emergence and key fitness traits in these populations.
An interplay between extracellular signalling and the dynamics of the exit from pluripotency drives cell fate decisions in mouse ES cells
Authors: Turner David A. , Trott Jamie , Hayward Penelope , Rué Pau , Martinez Arias Alfonso 
Published: June 2014

ABSTRACT
Embryonic Stem cells derived from the epiblast tissue of the mammalian blastocyst retain the capability to differentiate into any adult cell type and are able to self-renew indefinitely under appropriate culture conditions. Despite the large amount of knowledge that we have accumulated to date about the regulation and control of self-renewal, efficient directed differentiation into specific tissues remains elusive. In this work, we have analysed in a systematic manner the interaction between the dynamics of loss of pluripotency and Activin/Nodal, BMP4 and Wnt signalling in fate assignment during the early stages of differentiation of mouse ES cells in culture. During the initial period of differentiation, cells exit from pluripotency and enter an Epi-like state. Following this transient stage, and under the influence of Activin/Nodal and BMP signalling, cells face a fate choice between differentiating into neuroectoderm and contributing to Primitive Streak fates. We find that Wnt signalling does not suppress neural development as previously thought and that it aids both fates in a context dependent manner. Our results suggest that as cells exit pluripotency they are endowed with a primary neuroectodermal fate and that the potency to become endomesodermal rises with time. We suggest that this situation translates into a “race for fates” in which the neuroectodermal fate has an advantage.
Target-induced DNAzyme Cleavage Accompanying Bioactive Enzymatic Assembly with Glucometer Readout for Quantitative Monitoring of Lead Ion
Authors: Liao Jia-Yao , Li Hang 
Published: June 2014

Abstract
Target-induced DNAzyme cleavage is designed for sensitive monitoring of lead ion with glucometer readout, coupling with an additional single-strand DNA accompanying glucoamylase assembly.
STUDENT POLITICS IN URBAN TERNATE, NORTH MALUKU
Authors: Amin Basri 
Published: June 2014

This article examines student politics articulated by university students in contemporary Ternate, North Maluku. The involvement of students in the political arena in the region is mostly organized through regional (ethnic) organizations.The larger context of such political process is decentralisation, which make religional resource resources dominated by the state. At the same time competition among local elites and ethnic groups flourish. This is the main background of a new formation of group interests in local level –-including local university students-- to gain group advantages. The case of Ternate, North Maluku, is an example of how groups of students organize their practical interests in the arena of politics by exploiting youth associations and ethnic organizations. Artikel ini mengkaji tentang politik yang diartikulasikan oleh kalangan mahasiswa dalam percaturan politik lokal di Ternate, Maluku Utara. Keterlibatan mahasiswa dalam arena kekuasaan di kawasan ini lebih banyak dilakukan melalui instrumen organisasi kedaerahan (etnis). Konteks besar yang menjadi landasan dari proses sosial ini adalah desentralisasi yang menempatkan sedemikian rupa sumberdaya pembangunan lebih banyak didominasi oleh negara, tapi pada saat yang sama perkembangan politik etnis terus menyertai persaingan kelompok dan elit lokal. Kasus Maluku Utara adalah sebuah contoh bagaimana kaum muda memainkan kepentingannya sendiri dalam percaturan kekuasaan dan dalam hal memanfaatkan kesempatan-kesempatan praktis untuk mereka.
Elevated serum levels of free triiodothyronine in adolescent boys with gynaecomastia compared with controls
Authors: Mieritz Mikkel G , Sorensen Kaspar , Aksglaede Lise , Mouritsen Annette , Hagen Casper P , Hilsted Linda , Andersson Anna-Maria , Juul Anders 
Published: June 2014

ObjectivePubertal gynaecomastia is a frequent phenomenon occurring in 20–40% of otherwise healthy adolescent boys. Little is known about the aetiology of pubertal gynaecomastia. Markedly elevated thyroid hormone levels in adults with hyperthyroidism are associated with gynaecomastia.DesignA cross-sectional examination of 444 healthy boys with and without pubertal gynaecomastia.MethodsWe evaluated TSH, triiodothyronine (T3), thyroxine (T4), free T4 and free T3 in a cohort of healthy boys with and without pubertal gynaecomastia.ResultsBoys with gynaecomastia had significantly higher serum free T3, even after correction for age, BMI and pubertal stage. After inclusion of IGF1 in the model the differences disappeared. TSH, T4, free T4 and T3 did not differ between the groups.ConclusionsWe speculate that the GH/IGF1 axis and thyroid hormones interact and influence the development of pubertal gynaecomastia.
Regulation of Inflorescence Branch Development in Rice Through a Novel Pathway Involving the Pentatricopeptide Repeat Protein sped1-D
Authors: Jiang Guanghuai , Xiang Yanghai , Zhao Jiying , Yin Dedong , Zhao Xianfeng , Zhu Lihuang , Zhai Wenxue 
Published: June 2014

Abstract
Panicle type has a direct bearing on rice yield. Here, we characterized a rice clustered-spikelet mutant, sped1-D, with shortened pedicels and/or secondary branches, which exhibits decreased pollen fertility. We cloned sped1-D and found that it encodes a pentatricopeptide repeat protein. We investigated the global expression profiles of wild-type, 9311, and sped1-D plants using Illumina RNA sequencing. The expression of several GID1L2 family members was downregulated in the sped1-D mutant, suggesting that the gibberellin (GA) pathway is involved in the elongation of pedicels and/or secondary branches. When we overexpressed one GID1L2, AK070299, in sped1-D plants, the panicle phenotype was restored to varying degrees. In addition, we analyzed the expression of genes that function in floral meristems and found that RFL and WOX3 were severely downregulated in sped1-D. These results suggest that sped1-D may prompt the shortening of pedicels and secondary branches by blocking the action of GID1L2, RFL, and Wox3. Moreover, overexpression of sped1-D in Arabidopsis resulted in the shortening of pedicels and clusters of siliques, which indicates that the function of sped1-D is highly conserved in monocotyledonous and dicotyledonous plants.
Cervical Cancer in Ethiopia: Survival of 1,059 Patients Who Received Oncologic Therapy
Authors: Kantelhardt Eva Johanna , Moelle Ulrike , Begoihn Matthias , Addissie Adamu , Trocchi Pietro , Yonas Bekuretsion , Hezkiel Petros , Stang Andreas , Thomssen Christoph , Vordermark Dirk , Gemechu Tufa , Gebrehiwot Yirgu , Wondemagegnehu Tigeneh , Aynalem Abreha , Mathewos Assefa 
Published: June 2014

Abstract

Background.
Almost 500,000 women are newly diagnosed with cervical cancer (CC) every year, the majority from developing countries. There is little information on the survival of these patients. Our primary objective was to evaluate consecutive CC patients presenting over 4 years at the only radiotherapy center in Ethiopia.

Methods.
All patients with CC from September 2008 to September 2012 who received radiotherapy and/or surgery were included (without brachytherapy). Vital status was obtained through telephone contact or patient cards.

Results.
Of 2,300 CC patients, 1,059 patients with standardized treatment were included. At the end of the study, 249 patients had died; surviving patients had a median follow-up of 16.5 months; the 10% and 90% percentiles were 3.0 and 32.7 months, respectively. Mean age was 49 years (21–91 years). The majority of patients presented with International Federation of Gynecology and Obstetrics stage IIb–IIIa (46.7%). Because of progression during the waiting time (median 3.8 months), this proportion declined to 19.3% at the beginning of radiotherapy. The 1- and 2-year overall survival probabilities were 90.4% and 73.6%. If assuming a worst-case scenario (i.e., if all patients not available for follow-up after 6 months had died), the 2-year survival probability would be 45.4%.

Conclusion.
This study gives a thorough 4-year overview of treated patients with CC in Ethiopia. Given the limited treatment availability, a relatively high proportion of patients survived 2 years. More prevention and early detection at all levels of the health care system are needed. Increasing the capacity for external-beam radiation as well as options for brachytherapy would facilitate treatment with curative intention.
Estimates of Young Breast Cancer Survivors at Risk for Infertility in the U.S.
Authors: Trivers Katrina F. , Fink Aliza K. , Partridge Ann H. , Oktay Kutluk , Ginsburg Elizabeth S. , Li Chunyu , Pollack Lori A. 
Published: June 2014

Abstract

Background.
Standard treatments for breast cancer can impair fertility. It is unknown how many U.S. survivors are at risk for infertility. We estimated the population at risk for infertility secondary to treatment among reproductive-aged breast cancer survivors.

Methods.
We combined data from three sources: the National Program of Cancer Registries (NPCR) and Surveillance, Epidemiology, and End Results cancer registry data on incident breast cancers diagnosed in women aged 15–44 years between 2004 and 2006; treatment data from NPCR's 2004 Breast and Prostate Cancer Data Quality and Patterns of Care (PoC) study; and data on women's intentions to have children from the 2006–2010 National Survey of Family Growth (NSFG).

Results.
In the cancer registry data, an average of 20,308 women with breast cancer aged &lt;45 years were diagnosed annually. Based on estimates from PoC data, almost all of these survivors (97%, 19,416 women) were hormone receptor positive or received chemotherapy and would be at risk for infertility. These women need information about the impact of treatments on fertility. Estimates based on NSFG data suggest approximately half of these survivors (9,569 women) might want children and could benefit from fertility counseling and fertility preservation.

Conclusion.
Nearly all young breast cancer survivors in the U.S. are at risk for infertility. Physicians should discuss the potential impact of treatment on fertility. A smaller but sizeable number of at-risk survivors may be interested in having children. Given the magnitude of potential infertility and its quality-of-life implications, these survivors should have access to and potential coverage for fertility services.
FOLFOX-4 Chemotherapy for Patients With Unresectable or Relapsed Peritoneal Pseudomyxoma
Authors: Pietrantonio Filippo , Maggi Claudia , Fanetti Giuseppe , Iacovelli Roberto , Di Bartolomeo Maria , Ricchini Francesca , Deraco Marcello , Perrone Federica , Baratti Dario , Kusamura Shigeki , Tamborini Elena , Castano Alessandra , Consonni Paola Valentina , Bossi Ilaria , Gavazzi Cecilia , Milione Massimo , Pelosi Giuseppe , de Braud Filippo 
Published: June 2014

Abstract

Purpose.
The standard treatment of peritoneal pseudomyxoma is based on cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy (HIPEC). The establishment of newer systemic treatments is an unmet clinical need for unresectable or relapsed peritoneal pseudomyxoma. The aim of our study was to assess the activity of chemotherapy with 5-fluorouracil and oxaliplatin (FOLFOX-4 regimen) in terms of response rate in this subset of patients.

Materials and Methods.
Patients were included in a single-center, observational study and treated with FOLFOX-4 administered every 2 weeks for up to 12 cycles or until progressive disease or unacceptable toxicity.

Results.
Twenty consecutive patients were reviewed from July 2011 to September 2013. Only partial responses were observed, with an objective response rate of 20%. Median progression-free survival and overall survival were 8 months and 26 months, respectively. Two patients were able to undergo laparotomy with complete cytoreduction and HIPEC in one case. Safety data for FOLFOX-4 were consistent with the literature. By means of a mutant enriched polymerase chain reaction, KRAS mutation was found in 16 of 19 cases (84%), and MGMT promoter methylation was found in 8 (42%, all KRAS mutant).

Conclusion.
FOLFOX-4 chemotherapy is tolerable and active in patients with peritoneal pseudomyxoma when disease is deemed unresectable or relapsed after peritonectomy and HIPEC. The identification of predictive biomarkers, such as KRAS for resistance to anti-epidermal growth factor receptor monoclonal antibodies and MGMT for response to temozolomide, is a priority for the development of evidence-based treatment strategies for peritoneal pseudomyxoma.
Morphological and molecular characterization of Posthodiplostomum sp. (Digenea: Diplostomidae) metacercaria in the muscles of snakeheads (Channa punctata) from Manipur, India
Authors: Athokpam V. , Tandon V. 
Published: June 2014

Abstract
The spotted snakehead, Channa punctata Bloch, 1793, is a locally important fish species commonly consumed by the natives in the state of Manipur, Northeast India. The fish host C. punctata from Lamphel area revealed a diplostomid metacercarial infection. Morphologically, the recovered metacercaria was identified as a species of Posthodiplostomum Dubois, 1936. Molecular characterization using the ribosomal RNA genes (rDNA 18S, ITS2 and 28S regions) and the mitochondrial CO1 region supplements the identification. Molecular analysis revealed the metacercaria to be closely related to Posthodiplostomum sp. Japan isolate, with sequence similarity variation from 97.5–99.7 % while considering for the three rDNA markers. The secondary structure of the ITS2 region further corroborated these results; the typical four-helix model, when compared to the taxon from Japan, showed differences only in twelve bases (with seven transitions and five transversions). In phylogenetic analysis also, the metacercaria claded with the genus Posthodiplostomum, coming closer to the Japanese isolate, thus supplementing the morphological identification of the metacercaria.
Characterization of Longidorus caespiticola (Nematoda: Longidoridae) from the Czech Republic
Authors: Kumari S. 
Published: June 2014

Abstract
Longidorus caespiticola was found for the first time from the rhizosphere of apple with two other Longidorus sp. at Žlunice, Czech Republic. Females and males were analyzed morphologically and morphometrically. Four regions (18S, ITS1, ITS2, D2-D3 expansion segments of 28S) of ribosomal DNA and one region (cox1) of mitochondrial DNA were sequenced and analyzed and compared with a population from the Slovak Republic. The partial mitochondrial cytochrome c oxidase subunit 1 (cox1) gene showed relatively high genetic variation compared with ribosomal DNA between the Czech and Slovakian populations.
Science, stories and the anti-vaccination movement
Authors: Veselková Marcela 
Published: June 2014

Abstract
This paper discusses the theoretical and methodological approaches to the study of the use or non-use of expert-based information in policy-making. Special attention is paid to the Narrative Policy Framework introduced by Jones & McBeth in 2010. This theory of the policy process adopts a quantitative, structuralist and positivist approach to the study of policy narratives. The Narrative Policy Framework is useful for the analysis of the use of expert-based information to resolve so-called wicked problems, which are characterized by intense value-based conflict between policy coalitions. The methodological approach of the Narrative Policy Framework is illustrated using the policy issue of mandatory vaccination.
Central civil service management structures as brokers of de-politicization and evidence based civil service management: A typology
Authors: Staňová Ľudmila 
Published: June 2014

Abstract
This article provides a methodological tool for studying central civil service management structures (CCSMS) and their role in de-politicizing the civil service, thereby contributing to evidence-based civil service management. This tool can be used in comparative studies of CCSMS across, but also within countries, since civil service management functions are often split between several central organizations in one country. The comparative study of CCSMS is particularly useful for contemporary policy makers in EU member states, who currently face the challenge of reforming civil service systems due to EU pressure. There is therefore an urgent need to establish which type of central structure would effectively support the EU’s favored values of professionalizing and de-politicizing the civil service. Stemming from theory on organizational autonomy, our typology focuses on two key dimensions of the CCSMS that support these values: decision-making autonomy over individual civil servants and structural accountability.
Researching social influences on decision making: The case for qualitative methods
Authors: Masaryk Radomír 
Published: June 2014

Abstract
Research on decision making has mainly been based on economic models that have tried to downplay the overall context of decision-making situations. When we look into the social influences on decision making we realize it is crucial that we bring the issue of context back into the spotlight. In the present paper we explore the methodological foundations of selected qualitative approaches for studying social influences on decision-making, focusing especially on their strengths and weaknesses. We conclude that this area has great potential for further research providing academic rigor is maintained when using qualitative methods.
Can a magic wand plausibly be used in serious psychological research? The complications of researching the ideal age at which to be a parent through the eyes of the child
Authors: Konečná Hana , Kocourková Jiřina , Burcin Boris , Kučera Tomáš , Davidová Karolína 
Published: June 2014

Abstract
There is a growing trend in European countries for childbearing to occur later in women’s lives. The recent increase in the use of ART, together with the long-term trend towards later childbearing, raises questions as to the acceptable age of childbearing in contemporary society. ART legislation varies considerably across Europe and age limits for access to fertility treatment are rarely defined. The legislation takes into account the preferences of potential parents; children’s preferences, however, are not ascertained. The article discusses a research method used in a survey of older children and young adults. The objective of the survey was to answer the following questions: What age would children prefer their parents to be if they could choose? What are their reasons? Respondents were asked the following question: “How old would you like your mother and father to be when you are 20 (version for respondents younger than 16) or 25 (version for those older than 16) if you could wave a magic wand?” Furthermore, their reasons for wishing a change were identified through an open question, “Why would you like to change the age of your parents?”
Analysis of selected compounds in the surface water of lake Oleckie Wielkie
Authors: Kluska Mariusz , Witkowska-Krajewska Ewa , Marciniuk-Kluska Anna 
Published: June 2014

Abstract
The paper presents the results of research on the content of 16 PAH, selected anions and heavy metals in samples collected from Lake Oleckie Wielkie. The samples were collected at seven locations around the lake within about 15 m from the shore in November 2012. The highest total concentration of 16 PAH (68.58 μmol dm−3) was found in water sample no. 7, and the lowest in water samples no. 1, 4 and 5. The obtained results do not exceed the levels of standards accepted in Poland for the surface water. The analysis of collected water samples, performed with the isotachophoresis method, showed a low content (less than 4 mg dm−3) of the analyzed anions: nitrates(V), nitrates(III), chlorides, sulphates(VI), sulphates(IV), phosphates(V) and hydrocarbonates(IV), as well as a low content of the analyzed heavy metals.
Larval development and settlement of the barnacle Amphibalanus amphitrite from the Red Sea: Influence of the nauplii hatching season
Authors: Al-Aidaroos Ali , Satheesh Sathianeson 
Published: June 2014

Abstract
Barnacle Amphibalanus amphitrite adults were collected from the Jeddah coast of the Red Sea during different seasons. The nauplii released by adults in autumn, winter, spring and summer were reared under laboratory conditions to know the larval development duration and settlement in relation to the hatching season. The nauplii reared during winter (11 days) and autumn (13 days) took longer to reach the cypris stage compared to nauplii reared in summer (6 days) and spring (7 days). The most successful settlement of larvae was observed in spring and summer and the least successful — in winter. The observations of gonads showed that summer and spring are the active breeding season for A. amphitrite in the Red Sea. The results of this study indicated that the nauplii hatching season plays a significant role in the larval development and settlement of barnacles in the Red Sea.