bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgenic expression of the proneural transcription factor Ascl1 in Müller glia stimulates retinal regeneration in young mice
Authors: Ueki Yumi , Wilken Matthew S. , Cox Kristen E. , Chipman Laura , Jorstad Nikolas , Sternhagen Kristen , Simic Milesa , Ullom Kristy , Nakafuku Masato , Reh Thomas A. 
Published: October 2015

Significance
The retina is subject to a variety of insults that lead to degeneration of one or more types of neurons and ultimate visual impairment and blindness. Although the retinas of nonmammalian vertebrates can regenerate new neurons after injury, mammalian retinas largely lack this potential. We have tested whether the expression of the proneural transcription factor Ascl1 may be a key difference between the fish and mouse by targeting this factor to the cells that provide new retinal progenitors in mature retina, the Müller glia. Our results show that at least one of the differences between mammal and fish Müller glia that bears on their difference in regenerative potential is the proneural transcription factor Ascl1.
Molecular signatures of plastic phenotypes in two eusocial insect species with simple societies
Authors: Patalano Solenn , Vlasova Anna , Wyatt Chris , Ewels Philip , Camara Francisco , Ferreira Pedro G. , Asher Claire L. , Jurkowski Tomasz P. , Segonds-Pichon Anne , Bachman Martin , González-Navarrete Irene , Minoche André E. , Krueger Felix , Lowy Ernesto , Marcet-Houben Marina , Rodriguez-Ales Jose Luis , Nascimento Fabio S. , Balasubramanian Shankar , Gabaldon Toni , Tarver James E. , Andrews Simon , Himmelbauer Heinz , Hughes William O. H. , Guigó Roderic , Reik Wolf , Sumner Seirian 
Published: October 2015

SignificanceIn eusocial insect societies, such as ants and some bees and wasps, phenotypes are highly plastic, generating alternative phenotypes (queens and workers) from the same genome. The greatest plasticity is found in simple insect societies, in which individuals can switch between phenotypes as adults. The genomic, transcriptional, and epigenetic underpinnings of such plasticity are largely unknown. In contrast to the complex societies of the honeybee, we find that simple insect societies lack distinct transcriptional differentiation between phenotypes and coherently patterned DNA methylomes. Instead, alternative phenotypes are largely defined by subtle transcriptional network organization. These traits may facilitate genomic plasticity. These insights and resources will stimulate new approaches and hypotheses that will help to unravel the genomic processes that create phenotypic plasticity.
Theory for rates, equilibrium constants, and Brønsted slopes in F
1
-ATPase single molecule imaging experiments
Authors: Volkán-Kacsó Sándor , Marcus Rudolph A. 
Published: October 2015

Significance
The biological function in the F1-ATPase enzyme can be elucidated by understanding the dynamical interplay of reactions and mechanical rotation in the protein. Recent single molecule imaging and rotor manipulation experiments revealed that the rate constants of enzymatic steps, such as ATP binding or phosphate release, show an exponential dependence on the stalled rotor angle. Following concepts that originated in theories of electron and group transfer reactions, a structure-based analytic model is described to treat the dynamical behavior observed in these experiments. Using biochemical and the single molecule observations, thermodynamic driving potentials are constructed that take into account the elasticity of the rotor shaft. The model predicts observable quantities without using adjustable parameters and is tested using existing data.
The human gene damage index as a gene-level approach to prioritizing exome variants
Authors: Itan Yuval , Shang Lei , Boisson Bertrand , Patin Etienne , Bolze Alexandre , Moncada-Vélez Marcela , Scott Eric , Ciancanelli Michael J. , Lafaille Fabien G. , Markle Janet G. , Martinez-Barricarte Ruben , de Jong Sarah Jill , Kong Xiao-Fei , Nitschke Patrick , Belkadi Aziz , Bustamante Jacinta , Puel Anne , Boisson-Dupuis Stéphanie , Stenson Peter D. , Gleeson Joseph G. , Cooper David N. , Quintana-Murci Lluis , Claverie Jean-Michel , Zhang Shen-Ying , Abel Laurent , Casanova Jean-Laurent 
Published: October 2015

Significance
The protein-coding exome of a patient with a monogenic disease contains about 20,000 variations, of which only one or two are disease causing. When attempting to select disease-causing candidate mutation(s), a challenge is to filter out as many false-positive (FP) variants as possible. In this study, we describe the gene damage index (GDI), a metric for the nonsynonymous mutational load in each protein-coding gene in the general population. We show that the GDI is an efficient gene-level method for filtering out FP variants in genes that are highly damaged in the general population.
LEO P: AN UNQUENCHED VERY LOW-MASS GALAXY*
Authors: McQuinn Kristen B. W. , Skillman Evan D. , Dolphin Andrew , Cannon John M. , Salzer John J. , Rhode Katherine L. , Adams Elizabeth A. K. , Berg Danielle , Giovanelli Riccardo , Girardi Léo , Haynes Martha P. 
Published: October 2015

ABSTRACT
Leo P is a low-luminosity dwarf galaxy discovered through the blind H i Arecibo Legacy Fast ALFA survey. The H i and follow-up optical observations have shown that Leo P is a gas-rich dwarf galaxy with active star formation, an underlying older population, and an extremely low oxygen abundance. We have obtained optical imaging with the Hubble Space Telescope to two magnitudes below the red clump in order to study the evolution of Leo P. We refine the distance measurement to Leo P to be 1.62 ± 0.15 Mpc, based on the luminosity of the horizontal branch stars and 10 newly identified RR Lyrae candidates. This places the galaxy at the edge of the Local Group, ∼0.4 Mpc from Sextans B, the nearest galaxy in the NGC 3109 association of dwarf galaxies of which Leo P is clearly a member. The star responsible for ionizing the H ii region is most likely an O7V or O8V spectral type, with a stellar mass ≳25 M
⊙. The presence of this star provides observational evidence that massive stars at the upper end of the initial mass function are capable of being formed at star formation rates as low as ∼10−5
M
⊙ yr−1. The best-fitting star formation history (SFH) derived from the resolved stellar populations of Leo P using the latest PARSEC models shows a relatively constant star formation rate over the lifetime of the galaxy. The modeled luminosity characteristics of Leo P at early times are consistent with low-luminosity dSph Milky Way satellites, suggesting that Leo P is what a low-mass dSph would look like if it evolved in isolation and retained its gas. Despite the very low mass of Leo P, the imprint of reionization on its SFH is subtle at best, and consistent with being totally negligible. The isolation of Leo P, and the total quenching of star formation of Milky Way satellites of similar mass, implies that the local environment dominates the quenching of the Milky Way satellites.
The Predictive Sciences
Authors: Lehoux Daryn 
Published: October 2015

Abstract
This article examines the science and folklore of Greek and Roman methods of weather prediction, dividing techniques into astrometeorological practices (those that looked at the motions of the stars and planets to predict the weather) and “Theophrastan” practices (those that looked at signs on earth or in the atmosphere, from the behavior of animals to the colors of clouds). It includes some discussion of how and when the ancients thought about the causal links between both kinds of signs and the ensuing weather, and discusses the uses of weather prediction in agriculture and seafaring, and its place in ancient didactic literature.
Burmese Buddhist Politics
Authors: Walton Matthew J. 
Published: October 2015

Abstract
This article looks thematically at several important aspects of Buddhist politics in Myanmar, from the precolonial period to the present. It considers a number of arguments regarding the use of Buddhism in both supporting and opposing political authority, especially as they are rooted in a dualistic conception of human nature. It presents several examples of Burmese Buddhist political thought that creatively combine traditional Buddhist ideas with other political ideologies and practices, revealing a once-vibrant tradition that will hopefully be revitalized with the country’s current political transition. The role of monks in politics is controversial in Myanmar, and the article looks at some of the unique aspects of monastic activism, using examples from the 2007 “Saffron Revolution” and the current anti-Muslim Buddhist nationalist movements. Finally, it offers several different strands of democratic thought, including a provocative Burmese Buddhist notion of “moral democracy.”
Early Enteral Nutrition in Children With Acute Pancreatitis
Authors: Abu‐El‐Haija Maisam , Wilhelm Rebecca , Heinzman Christie , Siqueira Bruna Nabuco Freire , Zou Yuanshu , Fei Lin , Cole Conrad R. 
Published: October 2015

ABSTRACTNutrition is an integral part of acute pancreatitis (AP) management and is not adequately studied in pediatrics. The goal from the present study was to evaluate the effect of nutrition and fat content on the length of stay and pain severity in patients with AP. This is a retrospective review of our nutrition database between May 1, 2014 and December 1, 2014. Pain levels were similar between patients who were allowed to feed and patients kept nill per os. Higher fat intake grams per kilogram per day was associated with significantly lower pain scores. Early feeds are feasible in pediatric patients with AP. Pain was not increased in the group that had more fat in their diet.
A process-output classification for customer participation in services
Authors: Dong Beibei , Sivakumar K. 
Published: October 2015

Purpose– The purpose of this paper is to propose a classification for customer participation (CP) in services. Furthermore, it develops research propositions examining the moderating role of the proposed classification on the link between the magnitude of CP and service outcomes.Design/methodology/approach– Building on the process-output framework, the paper explores the contingent nature of the effect of CP magnitude on service outcomes (satisfaction and efficiency).Findings– The research propositions suggest that specific output enhances the positive effect of CP magnitude on satisfaction but also intensifies the negative effect of CP magnitude on efficiency; conversely, generic output diminishes the positive effect of CP magnitude on satisfaction but mitigates the negative effect of CP magnitude on efficiency. The effect of CP magnitude on satisfaction is stronger for a structured participation process than for an unstructured process; while the negative effect of CP magnitude on efficiency is stronger for an unstructured participation process than for a structured process. Further, process structure has an asymmetric enhancing effect on the negative link between CP magnitude and efficiency such that the enhancing effect of process structure is stronger for specific output than for generic output; likewise, process structure has an asymmetric enhancing effect on the positive link between CP magnitude and satisfaction such that the enhancing effect of structure is stronger for generic output than for specific output.Research limitations/implications– The research provides a conceptual approach to classify CP. Further research can focus on empirical validation as well as expanding the scope and variables examined.Practical implications– The research points to guidelines to structure CP activities based on the nature of the participation process and the type of service output to achieve the competing goals of customer satisfaction and efficiency.Originality/value– The proposed classification offers a new method to visualize CP in services. The framework is applicable to a wide variety of services, service contexts, and resources contributed by customers during their participation.
Effects of Lowering Dialysate Calcium Concentration on Mineral and Bone Disorders in Chronic Hemodialysis Patients: Conversion from 3.0 mEq/L to 2.75 mEq/L
Authors: Yamada Shunsuke , Ueki Kenji , Tokumoto Masanori , Suehiro Takaichi , Kimura Hiroshi , Taniguchi Masatomo , Fujimi Satoru , Kitazono Takanari , Tsuruya Kazuhiko 
Published: October 2015

AbstractSelection of a lower dialysate calcium concentration (DCa) can reduce calcium burden and prevent vascular calcification in hemodialysis patients. However, decreased DCa can worsen mineral and bone disorders. This 1‐year retrospective observational study evaluated 121 hemodialysis patients at Fukuoka Renal Clinic who underwent conversion of DCa from 3.0 mEq/L to 2.75 mEq/L. The primary outcomes were changes in serum levels of calcium, phosphate, and parathyroid hormone (PTH). The effects of baseline serum calcium and PTH levels on changes in biochemical parameters were also determined. One year after DCa conversion, mean serum calcium level decreased, while serum phosphate, alkaline phosphatase, and PTH concentrations increased. The rate of achievement of target PTH was higher in patients with lower serum PTH level at baseline, while patients with higher baseline serum PTH level tended to exceed the upper limit of the PTH target range. Patients with higher baseline serum calcium concentration showed a greater decrease in serum calcium level and a greater increase in serum PTH level at 1 year. Patients with a lower baseline serum PTH level can benefit from optimal PTH control following conversion of DCa from 3.0 mEq/L to 2.75 mEq/L. However, secondary hyperparathyroidism may be exacerbated in some patients with higher baseline serum calcium (Ca) and PTH levels. These results indicate that an individualized approach can maximize the benefits of Ca unloading after conversion to lower DCa.
Patterns of IgE sensitization in house dust mite‐allergic patients: implications for allergen immunotherapy
Authors: Batard T. , Baron‐Bodo V. , Martelet A. , Le Mignon M. , Lemoine P. , Jain K. , Mariano S. , Horiot S. , Chabre H. , Harwanegg C. , Marquette C. A. , Corgier B. P. , Soh W. T. , Satitsuksanoa P. , Jacquet A. , Chew F. T. , Nony E. , Moingeon P. 
Published: October 2015

AbstractBackgroundUnderstanding patterns of IgE sensitization in Dermatophagoides‐allergic patients living in various geographical areas is necessary to design a product suitable for worldwide allergen immunotherapy (AIT).MethodsUsing a HIFI Allergy customized microarray assay, IgEs specific for 12 purified allergens from Dermatophagoides pteronyssinus or D. farinae were assessed in sera from 1302 house dust mite (HDM)‐allergic patients living in various areas. Comprehensive mass spectrometric (MS) analyses were conducted to characterize HDM extracts, as well as purified bodies and feces.ResultsPatterns of IgE reactivity to HDM allergens are comparable in all cohorts of patients analyzed, encompassing adults and 5‐ to 17‐year‐old children, as well as American, Canadian, European, and Japanese patients. Overall, >70% and >80% of HDM‐allergic patients are sensitized to group 1 and group 2 allergens, respectively, from D. pteronyssinus and/or D. farinae species. Furthermore, 20–47% of patients also have IgEs to allergens from groups 4, 5, 7, 13, 15, 21, and 23. All patients have IgEs to allergens present in mite bodies and feces. MS‐based analyses confirmed the presence of mite allergens recorded by IUIS in D. pteronyssinus and D. farinae extracts, with groups 2, 8, 10, 11, 14, and 20 prominent in bodies and groups 1, 6, 18, and 23 well represented in feces.ConclusionsMite‐specific AIT should rely upon a mixture of D. pteronyssinus and D. farinae extracts, manufactured from both feces and bodies. Such a combination is appropriate to treat children and adult Dermatophagoides‐allergic patients from Asia, Europe, and North America.
Alternative regimens of magnesium sulfate for treatment of preeclampsia and eclampsia: a systematic review of non‐randomized studies
Authors: Pratt Jeremy J. , Niedle Polina S. , Vogel Joshua P. , Oladapo Olufemi T. , Bohren Meghan , Tunçalp Özge , Gülmezoglu Ahmet Metin 
Published: October 2015

AbstractIntroductionThe optimal dosing regimen of magnesium sulfate for treating preeclampsia and eclampsia is unclear. Evidence from the Cochrane review of randomized controlled trials (RCTs) was inconclusive due to lack of relevant data.Material and methodsTo complement the evidence from the Cochrane review, we assessed available data from non‐randomized studies on the comparative efficacy and safety of alternative magnesium sulfate regimens for the management of preeclampsia and eclampsia. Sources included Medline, EMBASE, Popline, CINAHL, Global Health Library, African Index Medicus, Biological abstract, BIOSIS and reference lists of eligible studies. We selected non‐randomized study designs including quasi‐RCTs, cohort, case‐control and cross‐sectional studies that compared magnesium sulfate regimens in women with preeclampsia or eclampsia.ResultsOf 6178 citations identified, 248 were reviewed in full text and five studies of low to very low quality were included. Compared with standard regimens, lower‐dose regimens appeared equally as good in terms of preventing seizures [odds ratio (OR) 1.02, 95% confidence interval (CI) 0.46–2.28, 899 women, four studies], maternal morbidity (OR 0.47, 95%CI 0.32–0.71, 796 women, three studies), and fetal and/or neonatal mortality (OR 0.87, 95%CI 0.38–2.00, 800 women, four studies). Comparison of loading dose only with maintenance dose regimens showed no differences in seizure rates (OR 0.99, 95%CI 0.22–4.50, 146 women, two studies), maternal morbidity (OR 0.53, 95%CI 0.15–1.93, 146 women, two studies), maternal mortality (OR 0.63, 95%CI 0.05–7.50, 146 women, two studies), and fetal and/or neonatal mortality (OR 0.49, 95%CI 0.23–1.03, 146 women, two studies).ConclusionLower‐dose and loading dose‐only regimens could be as safe and efficacious as standard regimens; however, this evidence comes from low to very low quality studies and further high quality studies are needed.
Changes in dental fear among Finnish adults: a national survey
Authors: Liinavuori Arja , Tolvanen Mimmi , Pohjola Vesa , Lahti Satu 
Published: October 2015

AbstractObjectivesThe aim of this study was to evaluate changes in dental fear among adult Finns aged 19 years and over at the beginning of the study in 2000–2011 in a nationally representative sample.MethodsThe study was based on Health 2000 and 2011 Surveys by the National Institute for Health and Welfare in Finland. The Health 2000 Survey used a stratified two‐stage cluster sampling design (N = 9 922). Those who participated in 2000 (n = 7 964) were invited to participate in 2011. The number of participants was 3 961. Dental fear was assessed by a single question: ‘How afraid are you of visiting a dentist?’ (not at all, somewhat, very much). Background variables included were age (categories 30–34, 35–39, 40–44, 45–54, 55–64, 65–74, 75+ years), gender, marital status (nonsingle, single), and education (basic, secondary, higher). To match the population sizes in different areas and thus forming a nationally representative data, the data were weighted based on age, gender, and area. General linear modeling for repeated measures was used to evaluate changes in dental fear and associations with background variables.ResultsBetween 2000 and 2011, dental fear decreased more often than increased among adults in Finland. Change from being very afraid to not at all afraid of visiting a dentist was most common in the oldest group. For most participants, dental fear remained stable; being very afraid of visiting a dentist was more stable among participants from 35 to 54 years of age than among younger or older participants.ConclusionsDental fear is still common among adult Finns and it seems to depend on age. As the most obvious consequence of dental fear is avoidance of dental care, reducing dental fear should be taken into account when planning and reorienting oral healthcare services.
Financial costs for families of children with Type 1 diabetes in lower‐income countries
Authors: Ogle G. D. , Kim H. , Middlehurst A. C. , Silink M. , Jenkins A. J. 
Published: October 2015

AbstractAimsTo assess the direct costs of necessary consumables for minimal care of a child with Type 1 diabetes in countries where the public health system does not regularly provide such care.MethodsSupply costs were collected between January 2013 and February 2015 from questionnaires submitted by centres requesting International Diabetes Federation Life for a Child Program support. All 20 centres in 15 countries agreed to the use of their responses. Annual costs for minimal care were estimated for: 18 × 10 ml 100 IU/ml insulin, 1/3 cost of a blood glucose meter, two blood glucose test strips/day, two syringes/week, and four HbA1c tests/year. Costs were expressed in US dollars, and as % of gross national income (purchasing power parity) per capita.ResultsThe ranges (median) for the minimum supply costs through the private system were: insulin 10 ml 100 IU/ml equivalent vial: $5.10–$25 ($8.00); blood glucose meter: $15–$121 ($33.33); test strip: $0.15–$1.20 ($0.50); syringe: $0.10–$0.56 ($0.20); and HbA1c: $4.90–$20 ($9.75). Annual costs ranged from $255 (Pakistan) to $1,185 (Burkina Faso), with a median of $553. Annual % gross national income costs were 12–370% (median 56%). For the lowest 20% income earners the annual cost ranged 20–1535% (median 153%). St Lucia and Mongolia were the only countries whose governments consistently provided insulin. No government provided meters and strips, which were the most expensive supplies (62% of total cost).ConclusionsIn less‐resourced countries, even minimal care is beyond many families’ means. In addition, families face additional costs such as consultations, travel and indirect costs. Action to prevent diabetes‐related death and morbidity is needed.
Open‐label randomized non‐inferiority trial of a fixed‐dose combination of glimepiride and atorvastatin for the treatment of people whose Type 2 diabetes is uncontrolled on metformin
Authors: Ambery P. , Stylianou A. , Atkinson G. , Dott C. , Baylor Curtis L. , Haque N. , LaCroix K. , Min K. W. ,   on behalf of the Glimepiride/Atorvastatin Investigational Team
Published: October 2015

AbstractAimsTo evaluate, in a randomized, open‐label study, the non‐inferiority of a bioequivalent fixed‐dose combination of glimepiride and atorvastatin vs. separately co‐administered tablets in people with Type 2 diabetes mellitus.MethodsParticipants with HbA1c ≥ 53 to < 80 mmol/mol (≥ 7.0 to < 9.5%), average fasting blood glucose > 7.0 mmol/l, who were on metformin for ≥ 3 months, were randomized to combination (n = 215) or co‐administered glimepiride and atorvastatin (n = 212) once daily for 20 weeks. Up‐titration of glimepiride (1–4 mg) and atorvastatin (10–20 mg) were based on average fasting blood glucose and LDL cholesterol, respectively. Co‐primary endpoints were change from baseline to week 20 in HbA1c and LDL cholesterol.ResultsNon‐inferiority was demonstrated for both co‐primary endpoints: the upper limits of 95% CIs for differences (combination‐reference) were less than the prespecified margins of 3.3 mmol/mol (0.3%) for change from baseline in HbA1c [difference 0.1 mmol/mol (95% CI −1.6, 1.9); 0.01% (95% CI −0.15, 0.17)] and 6% for percentage change from baseline in LDL cholesterol [difference 0.87% (95% CI −2.47, 4.21)]. Similar proportions of participants on combination and reference had treatment‐emergent adverse events (64 vs. 61%). More participants on combination had hypoglycaemia (21 vs. 13%); most events were considered by the treating physician to be unrelated to study drug.ConclusionsThe combination was non‐inferior to separately co‐administered tablets and the safety profile was consistent with the known profiles of glimepiride and atorvastatin. The observed increase in hypoglycaemia on the combination cannot be explained, but may be attributable to non‐systematic collectiof glucose readings and may have been influenced by reporting bias in this open‐label trial.