bluuebunny/crossref_metadata_2025_split huggingface dataset:
Soil application of high-lignin fermentation byproduct to increase the sustainability of liquid biofuel production from crop residues
Authors: Lynd Lee , Kemanian Armen R , Smith Jo , Richard Tom L , Arifi Anela , Bozzetto Stefano , Fabbri Claudio , Field John , Pries Caitlin Hicks , Kubis Matt , Smith Pete , Wang Michelle , Hoey Madeline 
Published: July 2024

Abstract
When digestates from anaerobic digestion of crop residues are added to soil, a considerable body of information indicates that soil organic carbon (SOC) levels are comparable to those when crop residues are left in the field. This occurs although the amount of digestate added to soil is diminished by digestion and implies that digestion increases the proportion of carbon inputs stabilized as SOC. Here we examine the likelihood and implications of these features being manifested for soil application of high lignin-fermentation byproduct (HLFB) from liquid biofuel production. We show that steady-state SOC levels are much less sensitive to crop residue removal with HLFB return than without it, and provide an example supporting the feasibility of foregoing process energy and coproduct revenue when HLFB is returned to the soil. Informed by this review and analysis, we expect with moderate confidence that long-term SOC levels for soils amended with HLFB from some liquid cellulosic biofuel processes will not be substantially lower than those occurring when crop residues are left in the field. We have high confidence that the economically optimum rate of fertilizer nitrogen (N) application and N2O emissions will be lower at most sites for HLFB return to the soil than if crop residues were left in the field. We estimate that the per hectare N demand for processing crop residues to liquid biofuels is about a third of the per hectare demand for crop production, giving rise to an opportunity to use N twice and thereby realize cost savings and environmental benefits. These observations support but do not prove the hypothesis that a ‘win-win’ is possible wherein large amounts of liquid biofuel feedstock can be obtained from crop residues while improving the economics and sustainability of food and feed production. A research agenda aimed at exploring and testing this hypothesis is offered.
The quality and flavour effects of robusta coffee cultivated at various altitudes in Aceh Tengah District - Gayo Highlands were investigated
Authors: Sulaiman I , Hasni D , Husaini I , Octaviana Maliza Noer 
Published: July 2024

Abstract
Gayo Highland (GH) is known for its arabica GH also planted robusta in lower altitude areas, but its quality is rarely reported. In Indonesia, robusta is mostly planted in Lampung and Java and thus is well-examined and reported. This study explores the quality of the Gayo-robusta-picked coffee cherries and its green bean and the effect of post-harvest practices farmers apply towards the quality of Gayo robusta. This research used RBF Design with two factors and four repetitions. Factor A is altitude levels, which are 600-800 m.a.s.l, 801-1.000 m.a.s.l and 1.001-1.200 m.a.s.l. Factor B is post-harvest practices, which have two levels with and without post-harvest practices. The result shows that 800-1.000 m.a.s.l altitude showed better quality of red cherries (75.25% ± 3.30) as the black and green cherries percentages are lower than other levels. As the altitude increases, green beans tend to have higher moisture, defect value, and bigger size. The average moisture of the green bean was 12.44%, the defect value was 12.51%, and the green bean size that passed the 6.5 mm sieve was 86.84%. For the cupping test, the average score was 67.33. The best treatment was robusta was planted on 600-800 m.a.s.l and, with post-harvest practices, had a cup test value of 80.25 and was considered a quality of fine Robusta.
Evaluation of the invitro antidiabetic and anti-cholesterols activity of selected plant extract
Authors: Agustini S , Asmaliyah  , Hadi EEW , Imanullah A 
Published: July 2024

Abstract
Diabetic and hypercholesterolemia are the most common metabolic disorders in the world. The purpose of the study was to assess the anti-diabetic and anti-cholesterol effect of water leaf extract of Lagerstroemia speciosa, Cinnamomum parthenoxylon, and Oroxylum indicum in vitro. Those three plants are often used by the local community to treat diabetes and hypercholesterol. The water extract’s active ingredients are steroids and flavonoids. The flavonoid content was reported to be 51.89, 110.92, and 63.41 mg QE/g respectively. In vitro studies on hemoglobin glycosylation, showed that 30 ppm aqueous leaf extract of L. speciosa, C. parthenoxylon, and O. indicum showed considerable inhibition of hemoglobin glycosylation by 12.06 %, 33.25%, and 73.96%, compared to a positive control (metformin) was 30.6 %. The in vitro anti-cholesterol properties of O. indicum and C. parthenoxylon could lower cholesterol by 33% compared to simvastatin by 33.104%. According to the study’s findings, the water leaf extracts of O. indicum and C. parthenoxylon have excellent pharmaceutical potential and might be used as herbal remedy to manage diabetes and cholesterol. To verify this work, more in vitro and in vivo research is required.
Study of physical parameters on Sargassum liquid fertilizer production and its nutrient contents
Authors: Nurhayati  , Bhernama B G , Kurniawan H , Bellia S , Abad F , Asmara A P 
Published: July 2024

Abstract
Sargassum can be used as an alternative material for liquid organic fertilizer production. Liquid organic fertilizer is more environmental-friendly than the synthetic one. In the previous research, we have reported that Sargassum from Lange Beach (Aceh Besar, Aceh, Indonesia) was potential as a source of nutrients for plants. This current work aims to evaluate the levels of plants growth hormones including auxin, cytokinin, and gibberellin. Furthermore, we also evaluated the levels of macronutrients of the Sargassum liquid fertilizer (SLF) obtained from a twenty-five-day fermentation compared with the Indonesian Agricultural Ministry Regulation of Permentan no. 70/SR./140/10/2011. Using a high-performance liquid chromatography (HPLC) analysis, we reported that the levels of auxin, cytokinin, and gibberellin were 2.546 ± 0.399 ppm; 130.093 ± 0.378 ppm; and 13.456 ± 0.783 ppm, respectively. In addition, the contents of nitrogen (N), phosphorus (P), and potassium (K) as macronutrients were 1.79 ± 0.25 %; 0.025 ± 0.005 %; and 0.15 ± 0 %, respectively. Our study also measured the physical parameters of the reactor, such as temperature and humidity, when the production was taking place. Reactor temperature during the production process was in between 31 and 32°C. Meanwhile, the humidity was in between 87 and 89%. In conclusion, this work demonstrated that SLF produced from Lange’s Sargassum had sufficient nutrients needed by plants according to the national regulation.
Effect of variety and farm altitude on chemical content of Arabica coffee (Coffea arabica) grown in Gayo Highland, Indonesia
Authors: Abubakar Y , Alya M R , Karim A , Fazlina Y D 
Published: July 2024

Abstract
Arabica coffee grown in the Gayo Highlands, Aceh, by small holder farmers has been well known for its excellent quality and distinctive taste. Its physical and organoleptic qualities have been included in several reports. However, the literature on its chemical content, such as chlorogenic acid, caffeine, and antioxidant activity is very limited. The aims of this research are to investigate the effect of difference varieties and farm altitudes on the coffee chemical content, especially chlorogenic acid, caffeine, and antioxidant activity. This research used a factorial randomized block design with two factors, namely variety (V) and farm altitude (T). Coffee Varieties (V) consist of three most widely planted varieties in the Highlands, namely, Timtim (V1), Borbor (V2), and Ateng Super (V3). Farm altitude (T) consists of three levels, namely farm altitude between 1000-1200 m above sea level (asl), represented by Celala (T1), farm altitude between 1201-1400 m asl, represented by Pegasing (T2) and farm altitude between 1401-1600 m asl, which is represented by Permata (T3). The analysis of variance (ANOVA) showed that coffee varieties, farm altitudes and the interaction between the two factors had no significant effect on the parameters observed. However, the data show a tendency that caffeine content and antioxidant activity in ground coffee tends to increase as farm altitude increase, while the chlorogenic acid content tends to decrease. Overall, the chlorogenic acid content in ground coffee ranged between 3.36% - 7.82%, with an average of 5.78% ± 1.52%, caffeine content ranged between 1.35% - 1.82% with an average of 1.61% ± 0.24%, and antioxidant activity ranged between 62.55% - 79.32% inhibition, with an average of 69.23% ± 4.42%.
The effect of islamic ornament as secondary skin on daylight quality
Authors: Abrar M I , Munir A , Safwan  
Published: July 2024

Abstract
Islamic ornaments are used extensively in different types of buildings, serving both aesthetic and functional purposes. They are used in mosques, offices and other buildings, serving as both interior and exterior elements. One application is their use as secondary skins to cover window openings. This research investigates the effect of Islamic ornaments as secondary skins on interior lighting. The main question addressed is how Islamic ornaments affect the quality of natural lighting. Using computer simulations, scenarios with clear glass and varying ornament distance from the window were assessed under clear sky conditions at different times of the day. The results show that the presence of Islamic ornaments significantly reduces the average daylighting level on interior surfaces compared to scenarios without ornaments. Visual representations using ray diagrams and computer-generated images illustrate the effects of varying ornamental conditions. This research highlights the potential of Islamic ornaments to improve the quality of daylighting in architecture. The findings emphasise their role in optimising daylight indoors, and encourage careful consideration of the impact of spacing on window openings. In particular, the distribution of daylight varies between rooms orientated to the north and west.
Synthesis and physicochemical characterization of sago starch-porang glucomannan hydrogels crosslinked fumaric acid as a new material for drug delivery system
Authors: Salman  , Wahyuni F S , Suardi M , Djamaan A 
Published: July 2024

Abstract
Sago starch and porang glucomannan are natural polymers commonly used as pharmaceutical excipients. The nature of starch, which is easily damaged and has low stability, is an obstacle to its use as a drug-delivery polymer. Chemical modification with crosslinked fumaric acid produces hydrogels with controlled hydrophilicity. This research aimed to synthesize and evaluate the physicochemical characteristics of hydrogels from sago starch-porang glucomannan crosslinked with fumaric acid. Starch synthesis was carried out using the phase inversion method. Tests on the hydrogels formed from sago starch-porang glucomannan included solubility tests, swelling tests, FT-IR, SEM, and XRD analyses used to explore the hydrogels. The results of the swelling tests at 22 and 60 show an increase in the amount of water the hydrogels can absorb. Crosslinked through the development of esters is revealed by the ATR-FTIR spectrum in the fingerprint region. The results of the SEM test of hydrogels, sago starch, and porang glucomannan show a spherical to polyhedral shape with varying powder sizes. XRD analysis shows that the hydrogels from sago starch-porang glucomannan crosslinked with fumaric acid are semi-crystalline, and some formulas are amorphous. In conclusion, the synthesis of hydrogels from sago starch-porang glucomannan crosslinked with fumaric acid has characteristics that can be developed as a new material for drug delivery systems.
Some results from a transversely excited atmospheric carbon dioxide-laser induced breakdown spectroscopy investigation of tsunami deposit cored from Aceh Besar coastal region
Authors: Ramli M , Mitaphonna R , Kurihara K , Lahna K , Idris N 
Published: July 2024

Abstract
We undertook an extensive analysis to examine the geochemical imprints encapsulated within the samples of the 2004 Indian Ocean tsunami deposits procured from the Seungko Mulat coastal region, nestled in the western section of Aceh Besar Regency, Aceh Province, Indonesia. In order to induce the formation of micro-plasma, a Transversely Excited Atmospheric Carbon Dioxide-Laser Induced Breakdown Spectroscopy (TEA CO2-LIBS) has been employed as the excitation source. Our investigation unveils distinct spectral signatures within every layer of the stratified tsunami deposit. We observed the unmistakable presence of salt elements (Ca, K, Mg, Na, Al), transition metals, heavy metals (Si, Fe, Ti, Ba, Sr, Cu, Cr, Pb, Mn, Ni, V), and also organic constituents (C, H, O, and N). The emission intensities of these elements exhibited a significant increase within the layers of the tsunami deposit, unequivocally setting them apart from their non-tsunami equivalents. In addition, we performed a vertical analysis of LIBS elemental emission intensity ratios, unveiling unique values for the Ca/Ti, and Si/Ti, ratios, which significantly distinguish them from all other ratios scrutinized in this exhaustive investigation. This research serves to highlight the effectiveness of TEA-LIBS as a promising and efficient optical technique for discerning the chemical profile associated with the renowned 2004 Indian Ocean tsunami deposits from Seungko Mulat Village in Aceh Province.
Patchouli essential oil: Unlocking antimicrobial potential for dandruff control
Authors: Muhammad S , Isnaini N , Indra I , Prajaputra V , Sufriadi E , Ernawati E 
Published: July 2024

Abstract
Dandruff causes the scalp to peel and results in itching, which is caused by one of the fungi, Malassezia globosa. Several studies have revealed that the compounds in essential oils can act as antimicrobials. To broaden insights regarding the potential compounds in essential oils, this research focuses on the potential of patchouli essential oil in inhibiting the growth of dandruff-causing microbes. One of the compounds with antimicrobial potential in patchouli oil is patchouli alcohol. The steam distillation method was used for extraction oil from dried patchouli stems and leaves to produce three different types of oil samples: crude patchouli oil, heavy fraction patchouli oil, and light fraction patchouli oil. Subsequently, the GC-MS analysis was conducted to compare the compound contents in samples. Our results indicate that the three patchouli oil samples have varying patchouli alcohol contents. Crude patchouli oil contains 31.2% patchouli alcohol; heavy fraction patchouli oil contains 61.82% patchouli alcohol, while light fraction patchouli oil contains 23.99% patchouli alcohol. This study demonstrates that heavy fraction patchouli oil has a greater potential as an antimicrobial compared to crude patchouli oil and light fraction patchouli oil.
Seismic vulnerability distribution of the earthquake prone area in Central Aceh
Authors: Syifa S R , Muksin U 
Published: July 2024

Abstract
Central Aceh is known as an earthquake-prone area due to the presence of the Sumatra Fault and secondary fault crossing the area resulting the M 6.1 earthquake in 2013. The objective of the research is to investigate seismic vulnerability of Central Aceh. Microtremor observations were conducted at 25 locations in Central Aceh analysed using the Horizontal to Vertical Spectral Ratio (HVSR). Seismic amplification is represented by the maximum values of H/V. The dominant frequency f0 representing the sediment thickness was obtained when the H/V curve is maximum. The frequency values ranged from 14.9 to 25.9 Hz, and the seismic vulnerability values varied from 0.02 to 3.1. The top soil consists of thin layers of sedimentary material dominated by tertiary rocks. The rocks are classified as Type IV and Class I based on the dominant frequency values. This result is consistent with the geology, consisting of sedimentary and volcanic rocks. Areas with a seismic vulnerability value > 1.0 is considered to have a high risk of earthquake damage because they are likely to experience earthquake amplification. Areas with seismic vulnerability value > 1.0 are found in West Aceh and the Geureudong volcano area.
Application of the structural equation modeling-partial least square method to analyze factors affecting the quality of life of hypertension patients
Authors: Sofyan H , Inayati R , Harva F , Afriyan I , Yolanda Y 
Published: July 2024

Abstract
Hypertension is a serious health issue in Pidie Regency, Indonesia. This research utilizes Structural Equation Modeling - Partial Least Squares (SEM-PLS) method to analyze the factors contributing to hypertension in Pidie Regency. Primary data were was gathered via surveys and discussions held with inhabitants from the area. One of the multivariate statistical methods that can be used to explain these complex relationships is SEM-PLS. The direct effects of SEM-PLS analysis indicate the hidden demographic factors notably diminish the concealed variable of quality of life. The concealed indicators of medication adherence showcase a positive impact but lack statistical significance on the hidden variable of quality of life. Additionally, the latent activity variable has a positive and significant effect on quality of life. The research findings revealed an R2 value of 0.234, signifying that 23.4% of the variance in the latent quality of life variable can be explained by latent demographic, medication adherence, and activity variables.
Analysis of gravity anomaly data for identification of mineral distribution prospects in the Kuta Cot Glie area, Aceh Besar
Authors: Hasanah M , Syamsuddin F , Safitri R , Fadhli Z , Karmel M E R , Syukri M 
Published: July 2024

Abstract
Gravity anomaly data was analyzed using the gravity method to obtain variations in gravity values and to identify mineral distribution prospects in the Kuta Cot Glie area, Aceh Besar. The Scintrex CG-5 gravimeter instrument was used to measure gravity data. After being corrected and receiving complete Bouguer anomaly values, the variation in gravity values from the research location was shown to be in the range 75-126 mGal. There are two anomalies within gravity values that are higher and lower than the surroundings. The first one is the distribution of serpentine minerals (2.4 – 3.10 g/cm 3) with variations of 108-126 mGal. This serpentine mineral is located at the center of the research location with a total area of ± 67,500 m 2. The second anomaly is limonite mineral deposits (1.55-1.7 g/cm3) with variations in gravity values of 75-87 mGal. The limonite were found on the East and West sides of the location, with a distribution area of ±71,250 m 2. The mineral deposit of limonite is a result of weathering from serpentinite rocks, therefore, the location of limonite mineral deposits is lower than the location of serpentinite. These results are aligned with the geological conditions which is dominated by serpentinite ultramafic metamorphic rocks.
Accurately deciphering spatial domains for spatially resolved transcriptomics with stCluster
Authors: Wang Tao , Shu Han , Hu Jialu , Wang Yongtian , Chen Jing , Peng Jiajie , Shang Xuequn 
Published: July 2024

Abstract
Spatial transcriptomics provides valuable insights into gene expression within the native tissue context, effectively merging molecular data with spatial information to uncover intricate cellular relationships and tissue organizations. In this context, deciphering cellular spatial domains becomes essential for revealing complex cellular dynamics and tissue structures. However, current methods encounter challenges in seamlessly integrating gene expression data with spatial information, resulting in less informative representations of spots and suboptimal accuracy in spatial domain identification. We introduce stCluster, a novel method that integrates graph contrastive learning with multi-task learning to refine informative representations for spatial transcriptomic data, consequently improving spatial domain identification. stCluster first leverages graph contrastive learning technology to obtain discriminative representations capable of recognizing spatially coherent patterns. Through jointly optimizing multiple tasks, stCluster further fine-tunes the representations to be able to capture complex relationships between gene expression and spatial organization. Benchmarked against six state-of-the-art methods, the experimental results reveal its proficiency in accurately identifying complex spatial domains across various datasets and platforms, spanning tissue, organ, and embryo levels. Moreover, stCluster can effectively denoise the spatial gene expression patterns and enhance the spatial trajectory inference. The source code of stCluster is freely available at https://github.com/hannshu/stCluster.
High-throughput method for screening pendimethalin-degrading bacteria from one microbial bank
Authors: Peng Ya-Peng , Ma Liang , Huang Ying , Mo Ming-He , Liu Jian-Jin , Liu Tong 
Published: July 2024

Abstract
The extensive use of chemical pesticides, such as herbicides, has resulted in significant environmental pollution. Microbial degradation represents a crucial approach for managing this pesticide-associated pollution, with enrichment culturing serving as a method for isolating pesticide-degrading microorganisms. However, the efficiency of this strategy is limited, often yielding only a few isolated strains. In this study, a new mineral salt medium (MSM) was developed, and a high-throughput method was used for screening pendimethalin-degrading bacteria by measuring the bacterial growth in the MSM. The utilization of this method resulted in the isolation of 56 pendimethalin-degrading bacteria from approximately 2000 bacterial strains, including 37 Bacillus spp., 10 Alcaligenes spp., 5 Pseudomonas spp., and other 4 strains identified for the first time as pendimethalin-degrading strains. This method may hold promise not only for isolating bacterial strains capable of degrading other pesticides but also for facilitating the utilization of the substantial bacterial strains stored in bacterial banks.
Escherichia coli DNA replication: the old model organism still holds many surprises
Authors: Łazowski Krystian , Woodgate Roger , Fijalkowska Iwona J 
Published: July 2024

Abstract
Research on Escherichia coli DNA replication paved the groundwork for many breakthrough discoveries with important implications for our understanding of human molecular biology, due to the high level of conservation of key molecular processes involved. To this day, it attracts a lot of attention, partially by virtue of being an important model organism, but also because the understanding of factors influencing replication fidelity might be important for studies on the emergence of antibiotic resistance. Importantly, the wide access to high-resolution single-molecule and live-cell imaging, whole genome sequencing, and cryo-electron microscopy techniques, which were greatly popularized in the last decade, allows us to revisit certain assumptions about the replisomes and offers very detailed insight into how they work. For many parts of the replisome, step-by-step mechanisms have been reconstituted, and some new players identified. This review summarizes the latest developments in the area, focusing on (a) the structure of the replisome and mechanisms of action of its components, (b) organization of replisome transactions and repair, (c) replisome dynamics, and (d) factors influencing the base and sugar fidelity of DNA synthesis.