bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depression in Adults in the T1D Exchange Clinic Registry
Authors: Trief Paula M. , Xing Dongyuan , Foster Nicole C. , Maahs David M. , Kittelsrud Julie M. , Olson Beth A. , Young Laura A. , Peters Anne L. , Bergenstal Richard M. , Miller Kellee M. , Beck Roy W. , Weinstock Ruth S. ,   for the T1D Exchange Clinic Network
Published: May 2014

OBJECTIVE
Little is known about the frequency of depression in adults with type 1 diabetes (T1D) or its relationship to diabetes outcomes. The T1D Exchange clinic registry allowed us to explore depression in a large, heterogeneous sample.

RESEARCH DESIGN AND METHODS
Participants ≥18 years old (N = 6,172; median age 34 years; median diabetes duration 16 years; 55% female; and 89% non-Hispanic white) completed the eight-item Patient Health Questionnaire (PHQ-8), a validated, reliable measure of current depression. Probable major depression was defined in four ways: PHQ-8 ≥10, PHQ-8 ≥12, per diagnostic algorithm, and as a continuous variable. Characteristics and clinical outcomes of those with and without depression were compared using logistic and linear regression models.

RESULTS
A total of 4.6–10.3% of participants were classified as probable major depression depending on how defined. Participants classified as depressed were more likely female, nonwhite race/ethnicity, to have a lower household income and lower education level, to exercise less often, to miss insulin doses, and to have one or more complications (neuropathy, nephropathy, treatment for retinopathy, or cardiovascular/cerebrovascular disease) (all P &lt; 0.01). HbA1c was higher in the depressed versus not depressed groups (8.4 ± 1.7% [68 ± 8.6 mmol/mol] vs. 7.8 ± 1.4% [62 ± 15.3 mmol/mol]; P &lt; 0.001). Occurrence of one or more diabetic ketoacidosis events (11 vs. 4%; P &lt; 0.001) and one or more severe hypoglycemic events (18 vs. 9%; P &lt; 0.001) in the past 3 months was higher among depressed participants.

CONCLUSIONS
In the T1D Exchange clinic registry, adults with probable major depression have worse clinical outcomes than those not depressed. Whether identification and treatment of depression improves diabetes outcomes requires study. Depression is common in T1D, and better identification and treatment of this comorbid condition is needed.
Unnatural Deaths in a National Cohort of People Diagnosed With Diabetes
Authors: Webb Roger T. , Lichtenstein Paul , Dahlin Marie , Kapur Navneet , Ludvigsson Jonas F. , Runeson Bo 
Published: May 2014

OBJECTIVE
To examine risk of unnatural death among people diagnosed with diabetes irrespective of disease type.

RESEARCH DESIGN AND METHODS
We conducted a matched cohort study of the entire Swedish population using interlinked national registers. From the National Diabetes Register we identified 252,191 people diagnosed with diabetes (type 1 or 2) during 1996–2009. Each cohort member was matched for age, sex, and county of birth to five unaffected individuals randomly sampled from the Total Population Register. Mortality was examined with complete ascertainment, and risk ratios (RRs) for all unnatural deaths and for specific causes (suicide, accident, homicide, and iatrogenic effects) were estimated using conditional fixed-effects Poisson regression.

RESULTS
Risk of any unnatural death was elevated versus the general population: 77.3 versus 32.1 per 10,000 (RR 2.2 [95% CI 2.1–2.4]), and these deaths occurred at a younger age in the diabetes cohort. Risk was increased for suicide (RR 3.4 [95% CI 3.0–3.8]), accident (RR 2.0 [95% CI 1.9–2.1]), homicide (RR 3.1 [95% CI 1.6–6.1]), and iatrogenic effects (RR 2.4 [95% CI 1.9–3.2]). It was greatly elevated for fatal poisoning from a variety of agents, including psychotropic drugs and “other and unspecified medication,” as well as narcotics, alcohol, and carbon monoxide. Almost 9% of all fatal poisoning cases in the diabetes cohort were identified as overdoses of insulin or oral hypoglycemic drugs.

CONCLUSIONS
Various causes of unnatural death, in particular deliberate and accidental poisonings, occur more frequently among diabetic patients. Before preventive strategies can be implemented, a deeper understanding of the risk factors and causal mechanisms explaining the marked elevations in risk is needed.
Combined administration of bacteriocin- -producing, probiotic strain Enterococcus faecium CCM7420 with Eleutherococcus senticosus and their effect in rabbits
Authors: Simonová M. Pogány , Lauková A. , Chrastinová L. , Plachá I. , Strompfová V. , Čobanová K. , Formelová Z. , Chrenková M. 
Published: May 2014

AbstractThe effect of Enterococcus faecium CCM7420 (EF) - enterocin-producing and probiotic strain of rabbit origin, Eleutherococcus senticosus extract (ES) and their combination (ES+EF) was determined on selected bacteria in faeces and caecum content, leukocytes phagocytosis, blood biochemistry and growth performance. Ninety-six weaned rabbits were divided into 3 experimental (ES, EF, ES+EF) and control group (CG). The rabbits in the groups ES and EF+ES were fed commercial diet enriched with E. senticosus extract (30 g/100 kg feed), rabbits in groups EF and CG were fed untreated diet. The rabbits in the EF and ES+EF groups were administered with an overnight culture of E. faecium CCM7420 strain (500 μl/animal/day into water, 109 CFU/ml). The treatment period lasted 21 days. The microbiological examinations in faecal samples confirmed the presence of E. faecium CCM7420 strain. In groups EF and ES+EF, the reduction of faecal coliforms, Pseudomonas-like sp., Clostridium-like sp. and S. aureus was recorded. Leucocyte phagocytosis significantly increased in all experimental groups (P<0.0001) compared to CG. The lowest GPx values were measured in the ES+EF group. Higher total protein, triglycerides and calcium concentrations were detected in experimental groups compared to CG. The cholesterol concentration decreased in the ES group. The highest average daily gain was recorded in EF group; in ES+EF the better feed conversion ratio and no mortality was recorded. These results indicated that the dietary supplementation with the E. faecium CCM7420 and E. senticosus extract stimulate the leukocytes phagocytosis and reduces the potential pathogens in rabbits digestive tract without oxidative stress and improve the growth performance.
Immunohistochemical characteristics and distribution of neurons in the intramural ganglia supplying the urinary bladder in the male pig
Authors: Pidsudko Z. 
Published: May 2014

AbstractThis study investigated the distribution and chemical coding of neurons in intramural ganglia of the urinary bladder trigone (UBT-IG) and cervix (UBC-IG) in the male pig using combined retrograde tracing and double-labelling immunohistochemistry. Additionally, immunoblotting was used to confirm the presence of marker enzymes for main populations of autonomic neurons. Retrograde fluorescent tracer Fast Blue (FB) was injected into the wall of both the left and right side of the bladder trigone, cervix and apex during laparotomy performed under thiopental anaesthesia. Twelve μm-thick cryostat sections were processed for double-labelling immunofluorescence with antibodies against tyrosine hydroxylase (TH), dopamine β-hydroxylase (DBH), neuropeptide Y (NPY), somatostatin (SOM), galanin (GAL), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), calcitonin gene-related peptide (CGRP), substance P (SP) and vesicular acetylcholine transporter (VAChT). UBT-IG and UBC-IG neurons in both parts of the organ formed characteristic clusters (from few to tens of neuronal cells) found under visceral peritoneum or in the outer muscular layer. Immunohistochemistry revealed several subpopulations in UBT-IG and UBC-IG neurons, namely noradrenergic (ca. 76% and 76%), cholinergic (ca. 22% and 20%), non-adrenergic/non-cholinergic nerve cells (ca. 1.5% and 3.8%), NPY- (ca. 66% and 58%), SOM- (ca. 39% and 39 %), VIP- (ca. 5% and 0%) and NOS- immunoreactive (IR) (ca. 1.5% and 3.8%), respectively. Immunoblotting using antibodies to TH and VAChT showed the presence of studied proteins as revealed by the presence of protein bands of the correct molecular weight. This study has revealed a relatively large population of differently coded UBT- and UBC- IG neurons, which constitute an important element of the complex neuroendocrine system involved in the regulation of the male urogenital organs function.
Virulence factors and ability of staphylococci from bovine milk and the cowshed environment to biofilm formation
Authors: Kot B. , Binek T. , Piechota M. , Wolska K.M. , Zdunek E. , Płatkowska K. 
Published: May 2014

AbstractThe aim of this study was to examine virulence factors and the ability of S. aureus and CNS species isolated from milk of cows with mastitis to form biofilm, and to compare them with virulence factors of staphylococci from milk of cows without mastitis and cowshed environment. Most of S. aureus strains from cows with mastitis showed haemolytic activity (93.9%), among them 72.7% and 21.2% produced α- and β-haemolysin, respectively. S. aureus from cows with mastitis symptoms produced proteases (above 48%) and esterase (42.4%). The highly significant relationship between the number of S. xylosus strains producing haemolysins (62%) and the origin of these strains from milk of cows with mastitis was observed. The ability to produce proteases was significantly associated with S. sciuri from milk of cows with mastitis. The ability of biofilm formation by staphylococcal strains from milk of cows with mastitis was greater than in strains from milk of cows without mastitis and the difference was significant (p≤0.05). The highest percentage of strains from milk of cows with mastitis were weak biofilm formers (48.6%), while 40% and 11.4% of strains were moderate and strong biofilm producers, respectively. S. xylosus showed the highest ability to form biofilm, while the lowest ability to form biofilm was observed in S. aureus and S. epidermidis.In conclusion, production of exotoxins and enzymes, and ability of biofilm formation shown by many CNS isolated from milk of cows with mastitis symptoms indicates that these features are important in pathogenesis of this disease.
Preliminary studies on the reaction of growing geese (Anser anser f. domestica) to the proximity of wind turbines
Authors: Mikołajczak J. , Borowski S. , Marć-Pieńkowska J. , Odrowąż-Sypniewska G. , Bernacki Z. , Siódmiak J. , Szterk P. 
Published: May 2014

Abstract
Wind farms produce electricity without causing air pollution and environmental degradation. Unfortunately, wind turbines are a source of infrasound, which may cause a number of physiological effects, such as an increase in cortisol and catecholamine secretion. The impact of infrasound noise, emitted by wind turbines, on the health of geese and other farm animals has not previously been evaluated. Therefore, the aim of this study was to determine the effect of noise, generated by wind turbines, on the stress parameters (cortisol) and the weight gain of geese kept in surrounding areas. The study consisted of 40 individuals of 5- week- old domestic geese Anser anser f domestica, divided into 2 equal groups. The first experimental gaggle (I) remained within 50 m from turbine and the second one (II) within 500 m. During the 12 weeks of the study, noise measurements were also taken. Weight gain and the concentration of cortisol in blood were assessed and significant differences in both cases were found. Geese from gaggle I gained less weight and had a higher concentration of cortisol in blood, compared to individuals from gaggle II. Lower activity and some disturbing changes in behavior of animals from group I were noted. Results of the study suggest a negative effect of the immediate vicinity of a wind turbine on the stress parameters of geese and their productivity.
Prevalence of subclinical endometritis in repeat breeding cows and mRNA expression of tumor necrosis factor α and inducible nitric oxide synthase in the endometrium of repeat breeding cows with and without subclinical endometritis
Authors: Janowski T. , Barański W. , Łukasik K. , Skarżyński D. , Rudowska M. , Zduńczyk S. 
Published: May 2014

Abstract
Information on the prevalence of subclinical endometritis and its mechanism in repeat breeding cows is very limited. The aims of this study were: a) to evaluate the incidence of this disorder with cytobrush cytology b) to analyze mRNA expression of tumor necrosis factor α (TNFα) and inducible nitric oxide synthase (iNOS) in endometrial biopsy samples collected from repeat breeding cows with and without subclinical endometritis.
Two experiments were carried out. In experiment 1, 112 (12.4%) repeat breeding cows (inseminated at least 3 times and not pregnant) were selected out of 902 cows from 8 dairy herds. Cytobrush cytology was performed on these cows, using the threshold of 10% PMNs in uterine smears. The results showed that 45 out of the 112 cows (40.2%) were diagnosed as having subclinical endometritis. In experiment 2, uterine biopsy samples were taken from repeat breeding cows with subclinical endometritis (n = 10) and without this disorder (n = 10). Using reverse transcription- PCR, the mRNA expression of TNFα and iNOS was determined. A statistically significant increase in expression of both substances was measured in the group of cows with subclinical endometritis (p < 0.05).
These results provide evidence for a high prevalence of subclinical endometritis in repeat breeding cows as well as the involvement of TNFα and iNOS pathways in the regulation of this pathological condition.
The prevalence of dogs with lymphocyte proliferative responses to food allergens in canine allergic dermatitis
Authors: Kawano K. , Oumi K. , Ashida Y. , Horiuchi Y. , Mizuno T. 
Published: May 2014

Abstract
The aim of the present study was to examine the correlation between the results of lymphocyte proliferative test (LPT) specific to food allergens and allergic skin diseases in dogs. Investigations were performed in 138 dogs with allergic skin diseases diagnosed in a private animal hospital. Of the 138 animals, 97 cases had positive reactions in LPT specific to food allergens. Of these 97 dogs, 67 animals were diagnosed with canine atopic dermatitis (CAD), but 30 dogs did not have IgE antibodies to environmental allergens. As 14 dogs out of 30 animals showed a positive result, 12 dogs underwent elimination diet trial based on the test results and all of them showed improvement in the pruritus score. Therefore, we conclude that LPT is an effective diagnostic test for allergic skin disease. Results of the lymphocyte test are useful in the identification of food allergens for the elimination diet trial
Influence of importin α/β and exportin 1 on equine herpesvirus type 1 (EHV-1) replication in primary murine neurons
Authors: Słońska A. , Cymerys J. , Skwarska J. , Golke A. , Bańbura M.W. 
Published: May 2014

Abstract
Viruses replicating in the nucleus need to cross the nuclear membrane barrier during infection, therefore disruption of specific nuclear transport pathways is crucial for their replication cycle. In the present study we have investigated the influence of nucleo-cytoplasmic transport inhibitors - ivermectin and leptomycin B, on EHV-1 replication in primary murine neurons. Obtained results suggest that the examined proteins - exportin 1 and importin α/β may participate, but are not required, during EHV-1 infection. Based on these results, it can be assumed that EHV-1 is able to use other receptors for nucleo-cytoplasmic transport.
Therapeutic effect of adipose-derived mesenchymal stem cell injection in horses suffering from bone spavin
Authors: Nicpoń J. , Marycz K. , Grzesiak J. 
Published: May 2014

Abstract
In this article we demonstrate the efficiency of autologous transplantations of adipose-derived mesenchymal stem cells for equine bone spavin treatment. Horses qualified to the study were divided into three groups: (i) research - treated with intra-articular injections of autologous stem cells, (ii) comparison treated with steroid drugs and (iii) control - untreated. All animals underwent comprehensive clinical examination before and after treatment. Our research confirms the long-term beneficial influence resulting from stem cell therapy in horse bone spavin treatment, in contrast to routine steroid usage.
Characteristics of Brucella strains isolated from animals in Poland
Authors: Szulowski K. , Iwaniak W. , Weiner M. , Złotnicka J. 
Published: May 2014

Abstract
A total of 42 Brucella strains were isolated from animals in Poland in years 2003-2012. Most of them (N=37) originated from wild animals, 3 from cattle, 1 from pig and 1 from sheep. The strains were characterised using both bacteriological and molecular (Bruce-ladder and MLVA) methods. The examinations revealed that all strains from wild boars, hares, cattle and pigs (N=41) had the same phenotypic characteristics and were classified as B. suis biovar 2. The remaining strain, isolated from sheep, was classified as B. ovis. The molecular examination showed that all B. suis biovar 2 strains, except one, had the same molecular profile as reference strain B. suis bv2 Thomsen. Different from the others strain originated from boars imported to Poland and its VNTR profile was typical for Iberian strains.
Chitosan – a promising biomaterial in veterinary medicine
Authors: Drewnowska O. , Turek B. , Carstanjen B. , Gajewski Z. 
Published: May 2014

Abstract
Biomaterials originate from natural substances and are widely used in medicine. Although they have to satisfy many conditions to be useful for treatment, more and more research is carried out with new types of biomaterials that can help replace various tissues such as tendons and bones. Chitosan is a very promising material, revealing unique features, which makes it useful for veterinary medicine - antimicrobial activity, biocompatibility, biodegradability. It is also known as good scaffold material, especially when combined with other polymers. This article describes chitosan as a biomaterial and tissue engineering scaffold with possible applications in veterinary medicine
Crill: A novel technique to characterize nano-ligno-cellulose
Authors: Osong Sinke H. , Norgren Sven , Engstrand Per , Lundberg Mathias , Hansen Peter 
Published: May 2014

Abstract
The CrillEye is a technique for qualitatively assessing loose slender and fibrillar particles created during pulping. It has also been demonstrated that the crill measurement technique can easily be used to measure the degree of fibrillation of mechanical pulp based nano-ligno-cellulose (NLC). The measurement technique is based on an optical response of a suspension at two wavelengths of light; UV and IR. The UV light contains information on both fibres and crill, while IR only contains information on fibres. The resolution on the CrillEye module is based on optical response of the pulp and on an analogue signal analysis making it concentration independent. Characterization of particlesize distribution of nano-ligno-cellulose is both important and challenging. The objective of the work presented in this paper was to study the crill values of TMP and CTMP based nano-ligno-celluloses as a function of homogenization time. Results showed that the crill value of both TMP-NLC and CTMP-NLC correlated fairly well with the homogenization time.
A comparison of fibre deformations from mill like and laboratory kraft cooking of softwood
Authors: Salmén Lennart , Hornatowska Joanna 
Published: May 2014

Abstract
The fact that industrial pulps have a lower strength than their corresponding laboratory pulps is an unsolved problem affecting in various ways the potential fibre utilisation in different mills. The loss of pulp strength has to a great extent been attributed to changes at the fibre level. In order to clarify in what way changes in fibre properties contribute to the strength losses, cooking experiments were conducted using a laboratory batch digester in which mechanical forces may be introduced. Fibre properties, i.e. fibre structure and fibre strength, of laboratory-made pulps were compared with those of an industrial pulp. It was concluded that two essentially different mechanisms may be identified; one related to the transverse fibre shape, the other to fibre damage. The latter is manifested as lower rewetted zero-span strength which reduces tear resistance and tensile strength of the pulp. The former is a collapse of the fibre, reducing the lumen area and resulting in a pulp with lower water-retaining capacity, given sheets of lower density and a pulp that has to be beaten to a higher degree to reach the desired bonding and the desired tensile strength.
Automated Drop-on-Fiber contact angle measurement using a microrobotic platform
Authors: Saketi Pooya , Hirvonen Juha , Lai Yuli , Ganser Christian , Teichert Christian , Järnström Joakim , Fardim Pedro , Kallio Pasi 
Published: May 2014

Abstract
Measuring contact angles on single fibers enables the separation of structural factors from surface chemistry factors. Current Drop-on-Fiber (DOF) contact angle measurement methods for natural fibers generally, and for pulp/paper fibers specifically, present a number of challenges. These are manipulation and mounting of the microscale fibers, straightening natural fibers to produce repeatable axisymmetric droplet conformation, measuring the droplet volume excluding the fiber volume and also human errors due to manually performed tasks. This paper presents a novel method to measure contact angles in DOF systems and overcome the above mentioned challenges using microrobots. The proposed microrobotic platform is capable of handling natural and synthetic fibrous materials in microscale, and dispensing probe liquid droplets down to 12 nl. It measures contact angle values using computer vision and a droplet-profiledetection algorithm. It reports the contact angle values as a function of volume of the droplet. The paper validates the capabilities of the proposed platform by applying three commonly used probe liquids: deionized water, ethylene glycol and diiodomethane for measuring contact angles on glass and pulp fibers. Finally, the results are compared with a picoliter contact angle measurement approach.
Increase of the filler content in papermaking by using a silica-coated PCC filler
Authors: Lourenço Ana F. , Gamelas José A. F. , Ferreira Paulo J. 
Published: May 2014

Abstract
The increase of filler content in paper without significantly sacrificing the paper mechanical resistances is of high interest for papermakers. In this work, precipitated calcium carbonate (PCC) modified with silica was used as filler for papermaking. Handsheets based on a eucalyptus kraft pulp furnish with different amounts of the modified filler, ranging from 16 to 40%, were produced. For similar levels of filler content it was found that the strength properties of the handsheets produced with the modified PCC were always significantly better than those obtained with the unmodified PCC. In particular, for handsheets with a filler content of 30%, the tensile index and the Scott bond internal resistance improved by ca. 20% and 39%, respectively. Some decrease of the light scattering and opacity values was noted when using the modified PCC, but the brightness was roughly the same, for each filler level evaluated. The enhanced fibre-tofiller bonding can be due to the hydrogen bonding between the cellulosic fibres and the hydroxyl groups of the silica film coating the calcium carbonate crystals, during the sheet formation. The present results with the new modified PCC provide a possibility to increase the filler amount in the papermaking industry.