bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rendimiento del hongo comestible Pleurotus ostreatus cultivado en diferentes sustratos a base de residuos agroindustriales de la ciudad de Tacna
Authors: Valera López Anacelly , Castillo Cotrina Daladier , Tito Vargas Carlos 
Published: July 2024

La presente investigación tuvo como objetivo determinar el rendimiento del hongo comestible Pleurotus ostreatus cultivado sobre tres sustratos preparados a base de residuos agroindustriales de la ciudad de Tacna. En la experimentación se tuvo tratamientos con siete repeticiones con un total de 28 unidades experimentales distribuidas al azar. Los resultados obtenidos fueron: menor tiempo de corrida de micelio en el T2 (18 días) y mayor en el T4 (22 días); mayor número de hongos en el T1, T3 y T4 (control) con valores de 51, 50 y 46; tamaño de basidiocarpos en T1, T2, T3 y T4 de 5.8, 6.1, 5.9 y 5.9 cm respectivamente; y mejor eficiencia biológica en el T1 (87.6 %), T3 (76.5 %) y T4 (70.1 %); obteniéndose los mejores rendimientos en el T4 (26.9 %), T3 (24.1 %) y T1 (24.1 %). El valor de proteínas más alto fue en el T4 (10.07 %). Se concluye que el T1 formado por orujos de uva y coronta de maíz y el T3 formado por orujos de uva y orujos de aceituna fueron los más eficientes en rendimiento, eficiencia biológica y tasa de producción, no presentando diferencias significativas con el tratamiento control (T4).
Development of Electronic Teaching Materials Based on Contextual Teaching and Learning (CTL) Approach to Improve High School Students' Mathematical Problem Solving Skills
Authors: Azizah Nurul Izatul , Roza Yenita , Kartini Kartini 
Published: July 2024

The aim of this research is to develop approach-based electronic teaching materials Contextual Teaching And Learning (CTL) on systems of equations and linear inequalities to improve high school students' valid and practical mathematical problem solving abilities. This research method uses research and development with a 4D model. Data was collected using questionnaires, observations and interviews. The data analysis technique used is quantitative descriptive analysis. Teaching materials are said to be valid and practical if the score obtained is more than 70%. The results of this research show that electronic teaching materials are based The CTL approach was developed to increase mathematical problem solving abilities for high school students get score mean 89% with very valid category. The results of student responses in the limited trial obtained a score of 90.28% in the very practical category. The student response results in the field trial were 90.35% with very practical criteria. The teacher response results were 94.14% with the very practical category and the observation sheet 90.6% with the very practical criteria. From the results of validity and practicality, it can be seen that the teaching materials developed are suitable for use as learning resources.
Imaginação de dentro para fora, de fora para dentro
Authors: Jacques de Moraes Dalgalarrondo Luisa 
Published: July 2024

Este artigo pretende trazer algumas formas de entender a imaginação, principalmente através da leitura dos trabalhos dos filósofos Gaston Bachelard e Henry Corbin e articular esses entendimentos ao lugar ocupado pela imaginação nas artes da cena. Para tanto, será analisada a imaginação no trabalho de Konstantin Stanislavski, através de elementos como “as circunstâncias dadas”, o “e se…’mágico’” e o “filme de visões” e na prática de Michael Chekhov, com estratégias e conceitos como o “gesto psicológico” e o “corpo imaginário”.
Hydrogel-chitosan and polylactic acid-polycaprolactone bioengineered scaffolds for reconstruction of mandibular defects: a preclinical in vivo study with assessment of translationally relevant aspects
Authors: Ferrari Marco , Taboni Stefano , Chan Harley H. L. , Townson Jason , Gualtieri Tommaso , Franz Leonardo , Ruaro Alessandra , Mathews Smitha , Daly Michael J. , Douglas Catriona M. , Eu Donovan , Sahovaler Axel , Muhanna Nidal , Ventura Manuela , Dey Kamol , Pandini Stefano , Pasini Chiara , Re Federica , Bernardi Simona , Bosio Katia , Mattavelli Davide , Doglietto Francesco , Joshi Shrinidh , Gilbert Ralph W. , Nicolai Piero , Viswanathan Sowmya , Sartore Luciana , Russo Domenico , Irish Jonathan C. 
Published: July 2024

Background: Reconstruction of mandibular bone defects is a surgical challenge, and microvascular reconstruction is the current gold standard. The field of tissue bioengineering has been providing an increasing number of alternative strategies for bone reconstruction.Methods: In this preclinical study, the performance of two bioengineered scaffolds, a hydrogel made of polyethylene glycol-chitosan (HyCh) and a hybrid core-shell combination of poly (L-lactic acid)/poly (ε-caprolactone) and HyCh (PLA-PCL-HyCh), seeded with different concentrations of human mesenchymal stromal cells (hMSCs), has been explored in non-critical size mandibular defects in a rabbit model. The bone regenerative properties of the bioengineered scaffolds were analyzed by in vivo radiological examinations and ex vivo radiological, histomorphological, and immunohistochemical analyses.Results: The relative density increase (RDI) was significantly more pronounced in defects where a scaffold was placed, particularly if seeded with hMSCs. The immunohistochemical profile showed significantly higher expression of both VEGF-A and osteopontin in defects reconstructed with scaffolds. Native microarchitectural characteristics were not demonstrated in any experimental group.Conclusion: Herein, we demonstrate that bone regeneration can be boosted by scaffold- and seeded scaffold-reconstruction, achieving, respectively, 50% and 70% restoration of presurgical bone density in 120 days, compared to 40% restoration seen in spontaneous regeneration. Although optimization of the regenerative performance is needed, these results will help to establish a baseline reference for future experiments.
Fabrication and characterization of Ti–12Mo/xAl2O3 bio-inert composite for dental prosthetic applications
Authors: Yehia Hossam. M. , El-Tantawy Ahmed , Elkady Omayma A. , Ghayad Ibrahim M. , Daoush Walid M. 
Published: July 2024

Introduction: Titanium (Ti)-molybdenum(Mo) composites reinforced with ceramic nanoparticles have recently significant interest among researchers as a new type of bio-inert material used for dental prosthetic applications due to its biocompatibility, outstanding physical, mechanical and corrosion properties. The current work investigates the impact of alumina (Al2O3) nanoparticles on the properties of the Ti–12Mo composite, including microstructure, density, hardness, wear resistance, and electrochemical behavior.Methods: Ti–12Mo/xAl2O3 nanocomposites reinforced with different Al2O3 nanoparticles content were prepared. The composition of each sample was adjusted through the mechanical milling of the elemental constituents of the sample for 24 h under an argon atmosphere. The produced nanocomposite powders were then cold-pressed at 600 MPa and sintered at different temperatures (1,350°C, 1,450°C, and 1,500°C) for 90 min. Based on density measurements using the Archimedes method, the most suitable sintering temperature was found to be 1,450°C. The morphology and chemical composition of the milled and sintered composites were analyzed using back-scattering scanning electron microscopy (SEM) and X-ray diffraction (XRD).Results and Discussion: The results showed that the addition of Mo increased the Ti density from 99.11% to 99.46%, while the incorporation of 15wt% Al2O3 in the Ti–12Mo composite decreased the density to 97.28%. Furthermore, the Vickers hardness and wear behavior of the Ti–Mo composite were enhanced with the addition of up to 5 wt% Al2O3. The sample contains 5 wt% Al2O3 exhibited a Vickers hardness of 593.4 HV, compared to 320 HV for pure Ti, and demonstrated the lowest wear rate of 0.0367 mg/min, compared to 0.307 mg/min for pure Ti. Electrochemical investigations revealed that the sintered Ti–12Mo/xAl2O3 nanocomposites displayed higher corrosion resistance against a simulated artificial saliva (AS) solution than pure Ti. The concentrations of Ti, Mo, and Al ions released from the Ti–12Mo/xAl2O3 nanocomposites in the AS solution were within the safe levels. It was found from this study that; the sample of the composition Ti–12Mo/5wt%Al2O3 exhibited appropriate mechanical properties, biocompatibility, corrosion resistance against the AS solution with acceptable ion concentration released in the biological fluids. Therefore, it can be considered as a new bio-inert material for potential applications in dental prosthetics.
The inotropic and arrhythmogenic effects of acutely increased late INa are associated with elevated ROS but not oxidation of PKARIα
Authors: Gissibl Theresa , Stengel Laura , Tarnowski Daniel , Maier Lars S. , Wagner Stefan , Feder Anna-Lena , Sag Can Martin 
Published: July 2024

BackgroundAcute stimulation of the late sodium current (INaL) as pharmacologically induced by Anemonia toxin II (ATX-II) results in Na+-dependent Ca2+ overload and enhanced formation of reactive oxygen species (ROS). This is accompanied by an acute increase in the amplitude of the systolic Ca2+ transient. Ca2+ transient amplitude is determined by L-type Ca2+-mediated transsarcolemmal Ca2+ influx (ICa) into the cytosol and by systolic Ca2+ release from the sarcoplasmic reticulum (SR). Type-1 protein kinase A (PKARIα) becomes activated upon increased ROS and is capable of stimulating ICa, thereby sustaining the amplitude of the systolic Ca2+ transient upon oxidative stress.ObjectivesWe aimed to investigate whether the increase of the systolic Ca2+ transient as acutely induced by INaL (by ATX-II) may involve stimulation of ICa through oxidized PKARIα.MethodsWe used a transgenic mouse model in which PKARIα was made resistant to oxidative activation by homozygous knock-in replacement of redox-sensitive Cysteine 17 with Serine within the regulatory subunits of PKARIα (KI). ATX-II (at 1 nmol/L) was used to acutely enhance INaL in freshly isolated ventricular myocytes from KI and wild-type (WT) control mice. Epifluorescence and confocal imaging were used to assess intracellular Ca2+ handling and ROS formation. A ruptured-patch whole-cell voltage-clamp was used to measure INaL and ICa. The impact of acutely enhanced INaL on RIα dimer formation and PKA target structures was studied using Western blot analysis.ResultsATX-II increased INaL to a similar extent in KI and WT cells, which was associated with significant cytosolic and mitochondrial ROS formation in both genotypes. Acutely activated Ca2+ handling in terms of increased Ca2+ transient amplitudes and elevated SR Ca2+ load was equally present in KI and WT cells. Likewise, cellular arrhythmias as approximated by non-triggered Ca2+ elevations during Ca2+ transient decay and by diastolic SR Ca2+-spark frequency occurred in a comparable manner in both genotypes. Most importantly and in contrast to our initial hypothesis, ATX-II did not alter the magnitude or inactivation kinetics of ICa in neither WT nor KI cells and did not result in PKARIα dimerization (i.e., oxidation) despite a clear prooxidant intracellular environment.ConclusionsThe inotropic and arrhythmogenic effects of acutely increased INaL are associated with elevated ROS, but do not involve oxidation of PKARIα.
Early clinical outcomes of Portico and Edwards Sapien 3 valve prosthesis in transcatheter aortic valve replacement: propensity-matched analysis
Authors: Primessnig Uwe , Wiedenhofer Julia M. , Trippel Tobias D. , Loddenkemper Carina M. , Schrader Helene , Brand Anna , Spethmann Sebastian , Stangl Karl , Haghikia Arash , Landmesser Ulf , Boldt Leif-Hendrik , Blaschke Florian , Hindricks Gerhard , Sündermann Simon H. , Grubitzsch Herko , Falk Volkmar , Dreger Henryk , Sherif Mohammad 
Published: July 2024

IntroductionThere is a lack of real-world data directly comparing different valve prostheses for transaortic valve replacement (TAVR). We aimed to compare early clinical outcomes at 30-days between the self-expandable Portico valve (Abbott) with the balloon-expandable Edwards Sapien 3 valve (Edwards Lifesciences) (ES3).MethodsOut of 1,901 patients undergoing TAVR between January 2018 and December 2021, all patients who received either Portico valve or ES3 valve via transfemoral TAVR were matched using nearest-neighbor (1:1) propensity scoring. Primary endpoints were single safety endpoints and early safety composite endpoints defined by Valve Academic Research Consortium-2 (VARC-2) criteria. The secondary endpoint was to analyze risk predictors for new permanent pacemaker (PPM) implantation in TAVR.ResultsOut of 661 complete cases, a total of 434 patients were successfully matched based on age, sex, Euro Score II and STS-score. In the matched cohort, 217 received either a Portico or valve and 217 received an ES3 valve. The VARC-2 early safety composite scores indicated a significantly greater overall 30-day safety risk in the Portico group at 9.2% (n = 20) compared to 3.7% (n = 8) in the ES3 group (p = 0.032). The requirement for new permanent pacemaker (PPM) implantation was also higher in the Portico group, at 21.2% (n = 46) vs. 13.4% (n = 29) in the ES3 group (p = 0.042). 30-day mortality was higher was 3.7% (n = 8) in Portico group compared to 0.9% in ES3 group (p = 0.11). Furthermore, implantation of the Portico valve was identified as a significant risk predictor for new PPM implantation, alongside higher age, preprocedural atrioventricular block (AVB) and longer total procedure duration.ConclusionThis study shows significantly higher rates of early clinical complications for Portico valve prostheses compared to ES3. These findings should be especially taken into consideration when selecting valve prosthesis for high-risk patients.
Metabolomics and network pharmacology exploration of the effects of bile acids on carotid atherosclerosis and potential underlying mechanisms
Authors: Cheng Xing , Zhang Ruijing , Qi Xiaotong , Wang Heng , Gao Tingting , Zheng Lin , Qiao Maolin , Li Yaling , Gao Siqi , Chen Jinshan , Chang Runze , Zheng Guoping , Dong Honglin 
Published: July 2024

BackgroundBile acids (BAs), products of gut microbiota metabolism, have long been implicated in atherosclerotic disease pathogenesis. Characterizing the serum bile acid profile and exploring its potential role in carotid atherosclerosis (CAS) development are crucial tasks.MethodsIn this study, we recruited 73 patients with CAS as the disease group and 77 healthy individuals as the control group. We systematically measured the serum concentrations of 15 bile acids using ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS/MS). Multivariate logistic regression and least absolute shrinkage and selection operator (LASSO) regression were applied to analyze the impact of bile acids on the disease and select the key BAs. The possible molecular mechanism was elucidated by network pharmacology.Results(1) The BA profile of patients with CAS significantly differed. (2) Multifactorial logistic regression analysis identified elevated levels of GCDCA (OR: 1.01, P &lt; 0.001), DCA (OR: 1.01, P = 0.005), and TDCA (OR: 1.05, P = 0.002) as independent risk factors for CAS development. Conversely, GCA (OR: 0.99, P = 0.020), LCA (OR: 0.83, P = 0.002), and GUDCA (OR: 0.99, P = 0.003) were associated with protective effects against the disease. GCA, DCA, LCA, and TDCA were identified as the four key BAs. (3) TNF, FXR, GPBAR1, ESR1 and ACE were predicted to be targets of BAs against AS. These four BAs potentially impact AS progression by triggering signaling pathways, including cAMP, PPAR, and PI3K-AKT pathways, via their targets.ConclusionThis study offers valuable insights into potential therapeutic strategies for atherosclerosis that target bile acids.
Effectiveness of baricitinib in acquired reactive perforating collagenosis: a case report
Authors: Zheng Jianfeng , Ding Yangfeng , Chen Yuanyuan , Shi Yuling , Gao Yunlu 
Published: July 2024

BackgroundAcquired reactive perforating collagenosis (ARPC) poses a clinical challenge with an unclear pathogenesis. This disease has been frequently proven resistant to immunosuppressive treatments, significantly affecting the quality of life of patients. In this report, we highlight the efficacy of baricitinib as a viable option for maintenance therapy in ARPC.Case summaryAn 81-year-old woman presented to our hospital with recurrent pruritus and cup-like ulcerated lesions on her trunk and limbs persisting for 1 year. She exhibited limited response to oral antihistamines and topical steroids. Past medical history revealed a prolonged history of coronary heart disease and type 2 diabetes spanning several years to decades. Histopathological examination revealed cup-shaped depressions filled with necrotic inflammatory debris. In the dermis, a mixed inflammatory infiltrate composed of lymphocytes and histiocytes was observed. Van Gieson staining indicated the elimination of fibrous tissue extending from the dermis into the epidermis. Consequently, a diagnosis of ARPC was established. Due to the inadequate response to conventional treatments and the severe itching, we initiated baricitinib therapy for ARPC, resulting in gradual symptom improvement. Follow-up assessments showed no adverse reactions and normal laboratory findings.ConclusionThe case report suggests that baricitinib might offer significant therapeutic benefits for ARPC.
Neoadjuvant immunochemotherapy improves clinical outcomes of patients with esophageal cancer by mediating anti-tumor immunity of CD8+ T (Tc1) and CD16+ NK cells
Authors: He Yunlong , Yang Depeng , Lin Xiaoyu , Zhang Jinfeng , Cheng Rui , Cao Liangyu , Yang Lijun , Zhang Mengmeng , Shi Xinyue , Jin Xiyun , Sun Handi , Sun Haoxiu , Zang Jingyu , Li Yu , Ma Jianqun , Nie Huan 
Published: July 2024

BackgroundEsophageal cancer (ESCA) is one of the most common tumors in the world, and treatment using neoadjuvant therapy (NT) based on radiotherapy and/or chemotherapy has still unsatisfactory results. Neoadjuvant immunochemotherapy (NICT) has also become an effective treatment strategy nowadays. However, its impact on the tumor microenvironment (TME) and regulatory mechanisms on T cells and NK cells needs to be further elucidated.MethodsA total of 279 cases of ESCA who underwent surgery alone [non-neoadjuvant therapy (NONE)], neoadjuvant chemotherapy (NCT), and NICT were collected, and their therapeutic effect and survival period were compared. Further, RNA sequencing combined with biological information was used to analyze the expression of immune-related genes. Immunohistochemistry, immunofluorescence, and quantitative real-time PCR (qRT-PCR) were used to verify the activation and infiltration status of CD8+ T and CD16+ NK cells, as well as the function and regulatory pathway of killing tumor cells.ResultsPatients with ESCA in the NICT group showed better clinical response, median survival, and 2-year survival rates (p &lt; 0.05) compared with the NCT group. Our RNA sequencing data revealed that NICT could promote the expression of immune-related genes. The infiltration and activation of immune cells centered with CD8+ T cells were significantly enhanced. CD8+ T cells activated by PD-1 inhibitors secreted more IFN-γ and cytotoxic effector factor cells through the transcription factor of EOMES and TBX21. At the same time, activated CD8+ T cells mediated the CD16+ NK cell activation and secreted more IFN-γ to kill ESCA cells. In addition, the immunofluorescence co-staining results showed that more CD276+ tumor cells and CD16+ NK cells were existed in pre-NCT and pre-NICT group. However, CD276+ tumor cells were reduced significantly in the post-NICT group, while they still appeared in the post-NCT group, which means that CD16+ NK cells can recognize and kill CD276+ tumor cells after immune checkpoint blocker (ICB) treatment.ConclusionNICT can improve the therapeutic effect and survival period of resectable ESCA patients. NICT could promote the expression of immune-related genes and activate CD8+ T and CD16+ NK cells to secrete more IFN-γ to kill ESCA cells. It provides a theoretical basis and clinical evidence for its potential as an NT strategy in ESCA.
Implication of the Annexin 1/FPR axis in leishmanial exosome-mediated Leishmania major skin hyperpathogenesis
Authors: da Silva Lira Filho Alonso , Lafleur Andrea , Alvarez Fernando , Piccirillo Ciriaco A. , Olivier Martin 
Published: July 2024

IntroductionExosomes produced by the protozoan parasite Leishmania (LeishEXO) are well-established drivers of virulence, though mechanisms underlying their exacerbation of experimental leishmaniasis remain elusive. Expression of Annexin A1 (ANXA1), a protein implicated in exosome-mediated pathologies and viral internalization, has been shown to correlate with cutaneous leishmaniasis severity. Given ANXA1’s regulation of myeloid cells – the canonical hosts for Leishmania – we studied the potential role of ANXA1 and its receptors FPR1/2 in exerting LeishEXO’s effects.MethodsMurine and in vitro ANXA1-/- models were used to study the generation of protective TH1 responses during experimental L. major infection with and without LeishEXO. Recruitment of inflammatory cells was assessed using a peritoneal cell recruitment assay and immunophenotyping, and production of inflammatory mediators was measured using a cytokine and chemokine array. Treatment of experimental models with FPR2 antagonist WRW4 and FPR1/2 agonist WKYMVm was used to delineate the role of the FPR/ANXA1 axis in LeishEXO-mediated hyperpathogenesis.ResultsWe established that ANXA1 deficiency prohibits LeishEXO-mediated pathogenesis and myeloid cell infection, with minimal alterations to adaptive and innate immune phenotypes. FPR2 blockade with WRW4 similarly inhibited leishmanial hyperpathogenesis, while direct activation of FPRs with WKYMVm enhanced infection and recapitulated the LeishEXO-mediated phenotype. This research describes LeishEXO’s utilization of the ANXA1/FPR axis to facilitate parasitic internalization and pathogenesis, which may be leveraged in the development of therapeutics for leishmaniasis.
Glucose-albumin ratio (GAR) as a novel biomarker of postoperative urinary tract infection in elderly hip fracture patients
Authors: Wang Wei , Tang Wanyun , Yao Wei , Lv Qiaomei , Ding Wenbo 
Published: July 2024

PurposePostoperative urinary tract infections (UTIs) worsen the prognosis of elderly patients with hip fractures. This study aimed to assess the predictive ability of blood-based biomarkers, specifically the glucose-albumin ratio (GAR), in predicting postoperative UTIs.MethodsA retrospective observational study of 1,231 patients from a Level I trauma center was conducted. We evaluated the prognostic and predictive value of 15 biomarkers, including the glucose-albumin ratio, in elderly patients with hip fractures. The primary outcome measure was the incidence of postoperative UTIs.ResultsThe glucose to albumin ratio transformed into GAR was superior to any other biomarker in predicting postoperative UTIs in elderly hip fracture patients (AUC = 0.756, p &lt; 0.001). Elevated GAR (using the best cut-off value of 0.18) was independently associated with postoperative UTIs (OR 3.20, 95% CI 2.23–4.58). Further analysis dividing GAR levels into four groups according to quartiles showed that compared to patients with GAR levels of Q1 (&lt; 0.14), GAR levels of Q2 (0.14–0.17; OR 2.11, 95% CI 1.07–4.15), Q3 (0.17–0.21; OR 3.36, 95% CI 1.74–6.52) and Q4 (&gt; 0.21; OR 7.55, 95% CI 3.84–14.83) patients had significantly higher odds of UTIs.ConclusionGAR holds potential as a novel biomarker for predicting postoperative UTIs in elderly patients with hip fractures.