bluuebunny/crossref_metadata_2025_split huggingface dataset:
Construction of microenvironment onto titanium substrates to regulate the osteoblastic differentiation of bone marrow stromal cells in vitro and osteogenesis in vivo
Authors: Lai Min , Cai Kaiyong , Hu Yan , Zhang Yuan , Li Liqi , Luo Zhong , Hou Yanhua , Li Jinghua , Ding Xingwei , Chen Xiuyong 
Published: August 2012

AbstractTo mimic the extracellular matrix of natural bone, apatite/gelatin composite was deposited onto nanostructured titanium substrates via a coprecipitation method, which was pretreated by potassium hydroxide and heat treatment to generate an anticorrosive nanostructured layer. The successful formation of the apatite/gelatin nanocomposite onto titanium surfaces was revealed by Fourier transform infrared spectroscopy, field‐emission scanning electron microscopy, atomic force microscopy (AFM), and thin film X‐ray diffraction (TF‐XRD) measurements, respectively. The immunofluorescence staining of vinculin revealed that the apatite/gelatin nanocomposite deposited titanium substrate was favorable for cell adhesion. More importantly, bone marrow stromal cells cultured onto the apatite/gelatin nanocomposite deposited titanium substrates displayed significantly higher (p < 0.05 or p < 0.01) proliferation and differentiation levels of alkaline phosphatase, mRNA expressions of osteocalcin (OC), osteopontin (OPN), and collagen type I (Col I), and OC content after culture for 7, 14, and 21 days, respectively, which was also revealed by the immunofluorescence analysis of OC and OPN expression. The deposition of apatite/gelatin nanocomposite improved bone density (p < 0.05) and bone‐implant contact rate (p < 0.05), which was reflected by microcomputed tomography analysis and histological evaluation in vivo using a rabbit model. This work provides an approach to fabricate high‐performance titanium‐based implants with enhanced bone osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 653–666, 2013.
Ten patient stories illustrating the extraordinarily diverse clinical features of patients with thrombotic thrombocytopenic purpura and severe ADAMTS13 deficiency
Authors: George James N. , Chen Qiaofang , Deford Cassie C. , Al‐Nouri Zayd 
Published: August 2012

AbstractPatients with thrombotic thrombocytopenic purpura (TTP) and severe ADAMTS13 deficiency are often considered to have typical clinical features. However, our experience is that there is extraordinary diversity of the presenting features and the clinical courses of these patients. This diversity is illustrated by descriptions of 10 patients. The patients illustrate that ADAMTS13 activity may be normal initially but severely deficient in subsequent episodes. Patients with established diagnoses of systemic infection as the cause of their clinical features may have undetectable ADAMTS13 activity. Patients may have a prolonged prodrome of mild symptoms with only microangiopathic hemolytic anemia and thrombocytopenia or they may have the sudden onset of critical illness with multiple organ involvement. Patients may die rapidly or recover rapidly; they may require minimal treatment or extensive and prolonged treatment. Patients may have acute and severe neurologic abnormalities before microangiopathic hemolytic anemia and thrombocytopenia occur. Patients may have concurrent TTP and systemic lupus erythematosus. Patients may have hereditary ADAMTS13 deficiency as the etiology of their TTP rather than acquired autoimmune ADAMTS13 deficiency. These patients' stories illustrate the clinical spectrum of TTP with ADAMTS13 deficiency and emphasize the difficulties of clinical diagnosis. J. Clin. Apheresis, 2012. © 2012 Wiley Periodicals, Inc.
Anti‐inflammatory activities of isorhamnetin‐3‐O‐galactoside against HMGB1‐induced inflammatory responses in both HUVECs and CLP‐induced septic mice
Authors: Kim Tae Hoon , Ku Sae‐Kwang , Bae Jong‐Sup 
Published: August 2012

AbstractHigh mobility group box 1 (HMGB1) protein is a crucial nuclear cytokine that elicits severe vascular inflammatory diseases. Oenanthe javanica (water dropwort) extract has anti‐arrhythmic, neuroprotective and anti‐diabetic activity. However, isorhamnetin‐3‐O‐galactoside (I3G), an active compound from O. javanica, is not researched well for its biological activity. Here, we investigated the anti‐inflammatory activities of I3G by monitoring the effects of I3G on the lipopolysaccharide (LPS) or cecal ligation and puncture (CLP)‐mediated release of HMGB1 and HMGB1 or CLP‐mediated modulation of inflammatory responses. I3G potently inhibited the release of HMGB1 and down‐regulated HMGB1‐dependent inflammatory responses in human endothelial cells. I3G also inhibited HMGB1‐mediated hyperpermeability and leukocyte migration in mice. Further studies revealed that I3G suppressed the production of tumor necrosis factor‐α and activation of nuclear factor‐κB by HMGB1. In addition, I3G reduced CLP‐induced HMGB1 release and sepsis‐related mortality. Given these results, I3G should be viewed as a candidate therapeutic agent for the treatment of severe vascular inflammatory diseases such as sepsis or septic shock via inhibition of the HMGB1 signaling pathway. J. Cell. Biochem. 114: 336–345, 2013. © 2012 Wiley Periodicals, Inc.
Role of bombesin receptor activated protein in the antigen presentation by human bronchial epithelial cells
Authors: Qu Xiangping , Li Menglan , Liu Hui‐Jun , Xiang Yang , Tan Yurong , Weber Horst Christian , Qin Xiao‐Qun 
Published: August 2012

AbstractBombesin receptor activated protein (BRAP) was identified in a bacterial two‐hybrid screen for proteins interacting with bombesin receptor subtype‐3 (BRS‐3). We found that BRAP is widely expressed in the airway epithelium of human lungs and may play a role during the stress response of lung epithelium. In this work, we explored the potential roles of BRAP in the antigen presenting function of human bronchial epithelial cells (HBECs). Overexpression of a BRAP recombinant protein in a human bronchial epithelial cell line resulted in a reduction of FITC‐OVA uptake by HBECs, which indicated that the antigen uptake ability is inhibited. The analysis of the protein expression of surface molecules including B7 homologs and the major histocompactability complex (MHC) class II molecules showed that the expression levels of HLA‐DR and B7DC increased while the levels of B7‐H1 and B7.2 decreased. Since those surface molecules are all related to antigen presenting process, the altered expression pattern of those molecules provides further evidence showing that BRAP overexpression leads to a change in antigen presenting function of HBECs. Moreover, overexpression of BRAP in HBECs caused a decrease of co‐cultured lymphocytes proliferation and a changed pattern of cytokines produced by lymphocytes in the presence of FITC‐OVA, which indicated that changes in the maturation pattern and functions of co‐cultured lymphocytes were induced by BRAP overexpression. Overall, our results suggested that overexpression of BRAP may play a role during the antigen presenting process of bronchial epithelium by inhibiting the antigen uptake ability of bronchial epithelial cells. J. Cell. Biochem. 114: 238–244, 2012. © 2012 Wiley Periodicals, Inc.
Expression pattern of Boule in dairy goat testis and its function in promoting the meiosis in male germline stem cells (mGSCs)
Authors: Li Mingzhao , Liu Chao , Zhu Haijing , Sun Junwei , Yu Meng , Niu Zhiwei , Liu Weishuai , Peng Sha , Hua Jinlian 
Published: August 2012

AbstractBoule is a conserved gene in meiosis, which encodes RNA binding protein required for spermatocyte meiosis. Deletion of Boule was found to block meiosis in spermatogenesis, which contributes to infertility. Up to date, the expression and function of Boule in the goat testis are not known. The objectives of this study were to investigate the expression pattern of Boule in dairy goat testis and their function in male germline stem cells (mGSCs). The results first revealed that the expression level of Boule in adult testes was significantly higher than younger and immature goats, and azoospermia and male intersex testis. Over‐expression of Boule promoted the expression of meiosis‐related genes in dairy goat mGSCs. The expression of Stra8 was up‐regulated by over‐expression of Boule analyzed by Western blotting and Luciferase reporter assay. While, Cdc25a, the downstream regulator of Boule, was found not to affect the expression of Stra8, and our data illustrated that Cdc25a did not regulate meiosis via Stra8. The expression of Stra8 and Boule was up‐regulated by RA induction. Taken together, results suggest the Boule plays an important role in dairy goat spermatogenesis and that over‐expression of Boule may promote spermatogenesis and meiosis in dairy goat. J. Cell. Biochem. 114: 294–302, 2013. © 2012 Wiley Periodicals, Inc.
LIGHT regulates the adipogenic differentiation of mesenchymal stem cells
Authors: Liu Changlong , Ding Huawei , Zhu Wenbo , Jiang Shaojie , Xu Jianrong , Zou Gang‐Ming 
Published: August 2012

AbstractLIGHT is a cytokine belonging to the TNF family. This cytokine has been extensively defined in its role on T‐cell regulation and dendritic cell maturation. It also exhibits the role in liver regeneration. We recently identified its role in regulation of hematopoietic stem cell differentiation. However, the question whether this cytokine regulates mesenchymal stem cells (MSCs) proliferation and/or differentiation remains unknown. In this study, we observed that MSCs express LT‐βR but not HVEM. PCR analysis show LIGHT mRNA is undectable in MSCs. LIGHT did promote neither MSCs proliferation nor migration. However, LIGHT promoted MSCs differentiation into adipocyte which was confirmed by Oil Red O Staining Assay. Since either MSCs or adipocytes are the major cell population in bone marrow niche, we then suggest that LIGHT regulate bone marrow niche, such as MSCs differentiation. J. Cell. Biochem. 114: 346–353, 2013. © 2012 Wiley Periodicals, Inc.
Comprehensive method based on model free method and IKP method for evaluating kinetic parameters of solid state reactions
Authors: Han Yunqing , Li Tianxiang , Saito Kozo 
Published: August 2012

AbstractThis article presents, firstly, a short review of methods for evaluating kinetic parameters of solid state reactions and a critical analysis of the isoconversional principle of model free methods. It shows theoretically that the activation energy for complex reactions is not only a function of the reaction degree but also of heating programs, and points out that any method that attempts to extract the dependences of activation energy on conversion degree without considering the dependences of heating programs is problematic. Then an analysis is given of the invariant kinetic parameters (IKP) method and recommends an incremental version of it. Based on the incremental IKP method and model free method, a comprehensive method is proposed that predicts the degree of the dependences of activation energy on heating programs, selects reliable values of activation energy and extracts the values of variable pre‐exponential factor. This comprehensive method is tested using both simulation data and experimental data, the results of which show it can not only give reliable values of kinetic parameters but also be helpful in explaining inconsistencies of kinetic results in solid state reactions. © 2012 Wiley Periodicals, Inc.
The Depression Anxiety Stress Scales—21 (DASS‐21): Further Examination of Dimensions, Scale Reliability, and Correlates
Authors: Osman Augustine , Wong Jane L. , Bagge Courtney L. , Freedenthal Stacey , Gutierrez Peter M. , Lozano Gregorio 
Published: August 2012

ObjectivesWe conducted two studies to examine the dimensions, internal consistency reliability estimates, and potential correlates of the Depression Anxiety Stress Scales—21 (DASS‐21; Lovibond & Lovibond, 1995).MethodParticipants in Study 1 included 887 undergraduate students (363 men and 524 women, aged 18 to 35 years; mean [M] age = 19.46, standard deviation [SD] = 2.17) recruited from two public universities to assess the specificity of the individual DASS‐21 items and to evaluate estimates of internal consistency reliability. Participants in a follow‐up study (Study 2) included 410 students (168 men and 242 women, aged 18 to 47 years; M age = 19.65, SD = 2.88) recruited from the same universities to further assess factorial validity and to evaluate potential correlates of the original DASS‐21 total and scale scores.ResultsItem bifactor and confirmatory factor analyses revealed that a general factor accounted for the greatest proportion of common variance in the DASS‐21 item scores (Study 1). In Study 2, the fit statistics showed good fit for the bifactor model. In addition, the DASS‐21 total scale score correlated more highly with scores on a measure of mixed depression and anxiety than with scores on the proposed specific scales of depression or anxiety. Coefficient omega estimates for the DASS‐21 scale scores were good.ConclusionsFurther investigations of the bifactor structure and psychometric properties of the DASS‐21, specifically its incremental and discriminant validity, using known clinical groups are needed.
The importance of key operational variables and electrolyte monitoring to the performance of an all vanadium redox flow battery
Authors: Watt‐Smith M.J. , Ridley P. , Wills R.G.A. , Shah A.A. , Walsh F.C. 
Published: August 2012

AbstractBACKGROUNDThe all vanadium redox flow battery (VRFB) has become the most common type of rfb, however, it is essential to improve our understanding of the importance of key operational variables, including electrode materials, electrolyte flow rate, current density and temperature, on the cell efficiency together with improved methods for cell monitoring.RESULTSSeveral carbon felts were characterized for their electrode activity. A commercially supplied electrolyte of unknown composition was analysed and was shown to contain a VO2+: V3+ ratio of 4.8:1 (total vanadium species = 1.5 mol dm−3) in 4 mol dm−3 H2SO4. A battery (100 cm2) was assembled using three‐dimensional carbon felts and planar carbon feeders as the electrodes with a Nafion® 115 proton exchange membrane. Performance was examined using electrolytes of varying vanadium ion concentration at volumetric flow rating from 0.5–3 mL min1. A suitable volumetric flow rate of electrolyte through each half‐cell was found to be in the range 1.5–2.0 mL min−1, corresponding to a mean linear electrolyte velocity of 1.0–10.1 cm s−1 through the carbon felt electrode. At a constant current charge and discharge current density of 100 mA cm−2 the typical voltage efficiencies were 65%. Charge‐discharge curves were then simulated using a detailed physical model, which generated good quantitative agreement with experimental cell performance.CONCLUSIONSThis study has quantified the effect of key process variables on cell efficiency. A mathematical model has been used successfully to describe charge‐discharge performance and the use of open‐circuit cell voltage has been shown to provide a simple and useful means of cell monitoring. Copyright © 2012 Society of Chemical Industry
Value‐added bioconversion of biomass by solid‐state fermentation
Authors: Chen Hong‐zhang , He Qin 
Published: August 2012

AbstractThe value‐added bioconversion of biomass is necessary due to the depletion of fossil fuels and deterioration of the global environment situation. Based on the analysis of characteristics of solid materials and the applicability of solid agro‐industrial residues used as feedstock for solid‐state fermentation (SSF), the authors established a value‐added bioconversion system for biomass using the key technology SSF. This article gives an overview of biomass bioconversion by SSF and the corresponding advances achieved in recent years. Copyright © 2012 Society of Chemical Industry
Formation of formic acid from glycerine using a hydrothermal reaction
Authors: Zhang Ya‐lei , Zhang Min , Shen Zheng , Zhou Jing‐fei , Zhou Xue‐fei 
Published: August 2012

AbstractBACKGROUND: Glycerine, a main by‐product of the biodiesel manufacturing process, has potential to be an important biorefinery feedstock with the rapid increase in biodiesel production all over the world. Hydrothermal experiments with glycerine were carried out at 250 °C using H2O2 as an oxidant.RESULTS: Glycerine was converted into formic acid with a yield of 31.0% based on the starting mass of carbon in glycerine. A possible oxidation pathway for the formation of formic acid from glycerine is proposed. In the proposed pathway, glycerine may first be oxidised and then decomposed into formic acid and oxalic acid. Oxalic acid was indirectly attributed to the increase of formic acid production from glycerine, but it instead acts as a retardant to prevent further oxidation of formic acid. However, when an alkali was added to the experimental conditions, the yield of formic acid was not greatly improved, reaching only 34.7%.CONCLUSION: The present work should help to facilitate further studies to develop a new green process for the production of formic acid from renewable biomass. © 2012 Society of Chemical Industry
A thermodynamic equilibrium consideration of the effect of sodium ion in acetoclastic methanogenesis
Authors: Oh Sung T. , Martin Alastair D. 
Published: August 2012

AbstractBACKGROUND: Sodium ion, a common constituent of food, is empirically observed to inhibit microbial activity (reduce bio‐kinetic rate) in anaerobic digestion. However such reductions may arise from a number of sources, including loss of enzyme activity and loss of thermodynamic driving force. In this work the theoretical thermodynamic approach was used to describe the inhibitory effects of sodium ion in acetoclastic and hydrogen utilizing methanogenesis.RESULTS: This work takes a modeling approach to investigate thermodynamic limitation by free energy, enthalpy and entropy analysis. Simple free energy analysis provides no evidence for thermodynamic limitation arising from even very high concentrations of sodium ion. However, entropic analysis suggests that increasing concentrations of sodium ion result in the loss of spontaneity. The loss of spontaneity arising from the presence of sodium ions is not directly related to the rise in equilibrium pH, which may also occur in the presence of sodium ions. The results also indicate that hydrogenophilic methanogens are less likely to be affected by the presence of sodium ion than their acetoclastic equivalents.CONCLUSION: From the thermodynamic perspective, the model advises that when the supply of thermal energy is sufficient, sodium inhibition under anaerobic conditions can be virtually completely overcome. The thermodynamic model also provides a design tool with which the stability of methanogenesis in response to feedstock mixtures containing sodium ion and a carbon source such as HAc can be investigated. © 2012 Society of Chemical Industry
Genistein affects ovarian folliculogenesis: A stereological study
Authors: Medigović Ivana , Ristić Nataša , Trifunović Svetlana , Manojlović‐Stojanoski Milica , Milošević Verica , Žikić Dragan , Nestorović Nataša 
Published: August 2012

AbstractThe effects of short‐term genistein exposure on ovarian folliculogenesis in immature rats were examined stereologically. To determine whether genistein acts as an estrogen agonist or antagonist, the results were compared with the effects of 17α‐ethynylestradiol. Immature female rats received 50 mg/kg/bw of genistein in dimethyl sulfoxide subcutaneously daily for three consecutive days from 18 to 20 days. The second group was injected with 1 μg/kg/bw of 17α‐ethynylestradiol in olive oil in the same schedule. Each group had a corresponding control. Genistein increased ovary and ovarian stroma volumes by 18.50% (P < 0.05) and 53.40% (P < 0.05), respectively, and changed the parenchyma to stroma ratio in favor of stroma. Genistein induced decreases in the number of primordial (by 17.23%; P < 0.05), primary (16.62%; P < 0.05), and secondary follicles (12.29%: P < 0.05), whereas the number of atretic secondary follicles increased (5.10‐fold; P < 0.05). The number of healthy large follicles was raised by 27.3% (P < 0.05), accompanied by 35.64% more atretic large follicles (P < 0.05). Similarly to genistein, estradiol changed the parenchyma to stroma ratio in favor of stroma, and reduced the number of primordial follicles, but the number of primary follicles was elevated. There were more healthy and atretic small and large follicles. In conclusion, genistein acted as an estrogen antagonist and had an inhibitory effect on the initial phase of folliculogenesis. In the other phases, genistein acted as an estrogen agonist, stimulating transition from the preantral to antral stage of folliculogenesis, and altering the ratio of follicular parenchyma and ovarian stroma in favor of stroma. Microsc. Res. Tech. 2012. © 2012 Wiley Periodicals, Inc.
Arboreal Habitat Structure Affects Locomotor Speed and Perch Choice of White‐Footed Mice (Peromyscus leucopus)
Authors: Hyams Sara E. , Jayne Bruce C. , Cameron Guy N. 
Published: August 2012

AbstractArboreal habitats pose several challenges for locomotion resulting from narrow cylindrical surfaces, steep slopes, and secondary branches that can form obstructions. We used laboratory trials to test whether different diameters, slopes, or complexity of branches affected maximum speeds and perch choice of the semi‐arboreal white‐footed mouse (Peromyscus leucopus). We tested locomotor performance of mice running horizontally and up and down 45° slopes for cylindrical artificial branches with five diameters ranging from 10 to 116 mm and on a subset of diameters for cylinders that were horizontal and had pegs (e.g., secondary branches) every 10 or 20 cm. Slope, diameter, and presence of pegs on top of cylinders had significant and interactive effects on locomotor performance. On horizontal cylinders the speed of mice increased with increased diameter among the three smallest diameters, but changed little with further increases in diameter, whereas for sloped surfaces the extreme diameters had lower speeds than an intermediate diameter. For a given diameter, the speeds of mice were usually faster when running horizontally rather than running uphill or downhill. The presence of pegs greatly decreased running speed compared to unobstructed surfaces, but the magnitude of this effect decreased as diameter increased. The difficulties of maintaining balance and avoiding toppling may have caused much of the decrease in speed and associated increased amounts of pausing. Only 1 of 11 choice tests detected a significant bias of mice favoring the perch that maximized locomotor performance. J. Exp. Zool. 317A:540–551, 2012. © 2012 Wiley Periodicals, Inc.