bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Adenoid Cystic Carcinoma Cell Line Possessing High Metastatic Activity has High NF‐kB Activation in Response to TNF‐α
Authors: Seki Katsuhiro , Ishii Kotaro , Sugiura Tsuyoshi , Takahashi Miho , Yoshiko Inoue , Shirasuna Kanemitsu 
Published: August 2012

AbstractAdenoid cystic carcinoma(AdCC)is characterized by frequent recurrence and distant metastasis. Although lung metastasis in AdCC is very common, the mechanism by which this occurs is uncertain. When five AdCC cell lines (ACCS, ACCT, ACCH, Acc‐3, and Acc‐M) were screened for metastatic ability by injecting tumor cells into nude mice via the tail vein, lung metastases were found in mice injected with Acc‐M (15/16 mice) but not in mice injected with any of the other four cell lines (0/10 mice with each line). To determine why Acc‐M metastasizes to the lung but the others do not, we examined the biological characteristics of Acc‐M and compared them with those of the other lines.Nuclear factor‐κB（NF‐κB）may play a key role in malignant tumor behaviors such as invasion and metastasis. Thus, we examined these cell lines for response to tumor necrosis factor（TNF‐α）, one of the typical stimulators of NF‐κB. Although treatment with TNF‐α stimulated matrix metalloprotease 9（MMP‐9）expression in all cell lines, the response to TNF‐α varied between cell lines ; the greatest stimulation was observed in Acc‐M. Acc‐M expressed higher levels of TNF receptors（both TNF‐R1 and TNF‐R2）than did the other AdCC lines. Judging from inhibitor‐κBα degradation and nuclear translocation and DNA binding by NF‐κB, the degree of activation of NF‐κB in response to TNF‐α in Acc‐M cell lines was very high compared to the other lines. Moreover, the ability of Acc‐M cells to adhere to endothelial cells, which was greater than that of the other cell lines, was further enhanced by pretreatment with TNF‐α. Acc‐M cells also expressed higher levels of sialyl Lewisx than did the other AdCC cell lines. These findings suggest that lung metastasis is mediated by tumor‐endothelial cell interaction, which is probably associated with the NF‐κB activation pathway. Further experiments are required to identify the molecules that mediate both lung metastasis and NF‐κB activation.
Progressive Hemifacial Atrophy Treated by Orthodontic Surgery
Authors: Kodama Yasumitsu , Miyazawa Marta , Fukuda Jun‐ichi , Iida Akihiko , Ono Kazuhiro , Takagi Ritsuo 
Published: August 2012

AbstractProgressive hemifacial atrophy（PHA）is a self‐limited atrophy of subcutaneous tissues（and less frequently of hard tissues）on one side of the face. It is a sporadic, slowly progressing disease whose pathogenesis is still unknown. As a rule the asymmetry caused by PHA（usually of soft tissue）is treated by volume augmentation that involves free tissue grafting or a pedicled flap transfer.We describe a rare case of hard tissue PHA. The atrophic changes occurred in the left molar tooth, maxilla and mandible and were associated with moderate soft tissue atrophy. The left side of the patient’s mouth was higher on the right side, and the occlusal plane was severely inclined in spite of normal occlusion. After no alteration and no progression of the atrophy were established, the patient was treated with orthodontic surgery. To correct the inclined occlusal plane and asymmetry profile, we performed a Le Fort I and intraoral vertical ramus osteotomy. In the 3‐year follow‐up, there were good occlusal balance and improved symmetrical profile without relapse or recurrence of the atrophy. Thus, orthodontic surgery was effective as a first procedure to treat hard tissue atrophy that appeared with moderate soft tissue atrophy.
Oral Leukoplakia Related to Malignant Transformation
Authors: Amagasa Teruo , Yamashiro Masashi , Ishikawa Hitoshi 
Published: August 2012

AbstractOral leukoplakia and its malignant transformation are reviewed in this article. Oral leukoplakia is defined as a predominantly white lesion of the oral mucosa that can not be characterized as any other definable lesion; however, the lesion must be confirmed histopathologically by biopsy in order to discuss malignant transformation of oral leukoplakia. Malignant transformation rates of oral leukoplakia range from 0.13 to 17.5%, while the rates of five‐year cumulative malignant transformation range from 1.2 to 14.5%. Some reports found a high incidence of malignant transformation in older patients. Chewing tobacco and smoking are distinct risk factors particularly among males in certain countries; however, other countries have noted that females or non‐smokers may be at risk of malignant transformation. HPV has been detected in oral dysplasia lesions and cancer in non‐smokers. Conflicting reports have been presented regarding the malignant transformation of oral leukoplakia with epithelial dysplasia; however, we and some authors believe that epithelial dysplasia is an important factor in the malignant transformation of oral leukoplakia. The majority of researchers showed non‐homogenous leukoplakia as a risk factor, although different terms have been used to describe these lesions. There may be several routes to malignant transformation of oral leukoplakia, including transformations induced by carcinogenesis due to betel quid chewing or smoking, or by HPV infection. While no definite treatment modalities for oral leukoplakia have been established, we suggest surgical therapy with an adequate safety‐margin and well‐timed evaluation as an appropriate treatment in preventing malignant transformation.
Developmental Signaling Disorders in Craniofacial Anomalies and Cancers
Authors: Zhang Yan , Wang Hua , Kamegai Akihide , Hata Tsuyoshi , Kitamura Naoya , Hosoda Masaru , Tani Ryouji , Hayashido Yasutaka , Toratani Shigeaki , Okamoto Tetsuji 
Published: August 2012

AbstractNormal human development requires the precise functioning and coordination of many complex pathways. Abnormalities in these signaling cascades often result in developmental perturbations, giving rise to congenital anomalies and cancers. There are 21,787 genes in each human nucleus, different gene subsets are expressed in different cell types, and different gene networks make different signal cascades. Among a large number of genes, in this review, we describe signaling disorders of sonic hedgehog and its receptor, patched‐1; Tie2 ; fibroblast growth factor receptor in craniofacial anomalies and oral cancers.
Hydrostatic Pressure Induces Cytokine Production in Human Periodontal Ligament Cells
Authors: Yamamoto Toshiro , Kita Masakazu , Kimura Isao , Oseko Fumishige , Amemiya Takeshi , Nakanishi Akira , Sakao Kei , Takahashi Kenji , Kubo Toshikazu , Kanamura Narisato 
Published: August 2012

AbstractPeriodontal tissue has a unique structure in that the human periodontal ligament (hPDL) lies between the hard tissues of cementum and alveolar bone. Although the role of cytokines in hPDL function is not clearly understood, we investigated the effect of mechanical stress as hydrostatic pressure (HP) on cytokine expression in hPDL cells.The hPDL cells were obtained from a healthy maxillary third molar. After the 3rd to 4th passage, the cells were exposed to HP ranging from 1 MPa to 6 MPa as previously described. The expression of cytokine mRNA was determined by RT‐PCR and cytokines in the culture supernatants were assessed by enzyme‐linked immunosorbent assay (ELISA).The exposure to 6 MPa of HP caused no morphological changes of hPDL cells, and did not affect cellular viability. No expression of IL‐1β, IL‐6, IL‐8, TNF‐α, RANK, RANKL or OPG mRNA was observed in the control cells under atmospheric pressure, whereas in hPDL cells treated with HP, enhancement of IL‐6, IL‐8, RANKL and OPG mRNA expression was observed between 10 and 60 minutes after the exposure to HP. After the exposure to HP, the production of IL‐6 and TNF‐α were induced significantly in hPDL cells, but IL‐1β and IL‐8 were not produced.These results suggest that hPDL cells may play a role in the production of cytokines in response to mechanical stress in vivo.
A Case of Symmetrical Lipomatosis of the Tongue Presenting as Macroglossia
Authors: Ishikawa Yoshimi , Katada Yu , Enomoto Masahiro , Kawada Kensuke , Okamoto Yoshiyuki , Ozawa Mikio 
Published: August 2012

AbstractSymmetrical lipomatosis is characterized by symmetrical infiltration of adipose tissue into the head, neck, or upper trunk. By contrast, oral involvement is extremely rare. The present report describes a case of symmetrical lipomatosis of the tongue (SLT) presenting as macroglossia. The patient was a 63‐yearold Japanese male who developed progressive dysarthria secondary to enlargement of the tongue. The patient's medical history was notable for alcoholic hepatitis and hyperlipidemia. Marginal glossectomy was performed on both sides of the tongue to achieve volume reduction and restore normal speech. Histopathologic examination of the resected tissue revealed diffuse infiltration with adipose tissue lacking a fibrous capsule. The patient's postoperative course was uneventful, and he remained free of recurrence or regrowth and functional deficits at the 6‐year follow‐up time point.
Successful Hemostasis after Dental Extraction with the Use of Recombinant Activated Factor VII in a Factor VII Deficient Patient
Authors: Koji Satoh , Masataka Okamoto , Aya Torimura , Rina Taguchi , Yasuhisa Mineno , Hideki Mizutani 
Published: August 2012

AbstractWe report our experience of successful hemostasis after dental extraction with the use of rFVII a in a FVII deficient patient. Preoperative PT% was 25%, and FVII was less than 3%. Thirty minutes before tooth extraction, 1.2 mg of rFVII a was injected. At the beginning of the operation, PT% was more than 200%, FVII was 336%, and the hemostasis after dental extraction was excellent. rFVII a was used effectively and safely for dental extraction in this case of FVII deficiency.
Ultrasonographic Evaluation and Differentiation of Tumorous Lesions in the Floor of the Mouth:
Authors: Ariji Yoshiko , Maeda Hatsuhiko , Naitoh Munetaka , Gotoh Masakazu , Izumi Masahiro , Aimiya Hidetoshi , Shimozato Kazuo , Kurita Kenichi , Ariji Eiichiro 
Published: August 2012

AbstractUltrasonography may provide some information as to the tissue characteristics of tumorous lesions in the floor of the mouth, which have not been sufficiently clarified. Ultrasonographic imaging characteristics of these lesions are presented and the differential diagnoses are discussed. Ultrasonographic images of 5 patients with metastatic lingual lymph nodes (squamous cell carcinoma), lymphangioma, Schwannoma, ranula and dermoid cyst are presented. The literature on the imaging features of tumorous lesions in the floor of the mouth was searched using Medline. Five cases of tumorous lesions in the floor of the mouth are presented. The differential diagnosis through a review of the references was discussed. Ultrasonographic images clearly showed the internal structures of the mass. The homogeneity varied according to the degree of closeness of the cells and tissues, or the presence of fluid, hemorrhage, cystic degeneration and calculus. The echogenicity was due to the high acoustic impedance of calculus, cholesterol, and so on. The imaging features varied according to the ratio of the tissues, such as fat and fibrous tissue (in lipoma), or cholesterol and keratin (in dermoid cyst). A high vascular mass indicated malignant salivary gland tumors and hemangioma. In conclusion, ultrasonographic images revealed the distinctive features of the lesions and were useful for the differential diagnosis. Therefore, ultrasonography could be used to conjecture the content of the lesions and is considered to be useful for easy and accurate diagnosis prior to treatment.
Etiology of Trigeminal Neuralgia
Authors: Toda Katsuhiro 
Published: August 2012

AbstractNeurovascular compression at the root entry zone accounts for more than 80% of trigeminal neuralgia (TN) cases, but not all patients with TN have neurovascular compression. Many non‐TN subjects have neurovascular contract at the root entry zone. TN is reported to occur in 0.9% to 4.5% of patients with multiple sclerosis (MS). In patients with TN, 1.7% to 15% of patients suffer from MS. The reported range for patients with TN due to tumors is from 0.8% to 11.6%. Because carbamazepine may relieve pain temporarily, relief of pain with carbamazepine does not exclude the diagnosis of a tumor or cyst. There are the peripheral cause theory, central cause theory, peripheral origin central pathogenesis theory, and multiple factors theory in the pathology of TN. Dental pain and/or treatment may trigger TM. Alveolar cavitational osteonecrosis may also cause TN.
Expression of Urokinase‐type Plasminogen Activator (uPA), uPA Receptor, and Plasminogen Activator Inhibitor‐1 in Oral Squamous Cell Carcinoma
Authors: Inoue Yoshiko , Sugiura Tsuyoshi , Matsuki Ryousuke , Ishii Kotaro , Seki Katsuhiro , Shirasuna Kanemitsu 
Published: August 2012

AbstractWe investigated whether the expression levels of urokinase‐type plasminogen activator (uPA), uPA receptor (uPAR), and plasminogen activator inhibitor‐1 (PAI‐1) correlate with clinicopathological features of oral squamous cell carcinoma (SCC). We immunohistochemically examined the expression levels of uPA, uPAR, and PAI‐1 in 160 biopsy specimens of oral SCC. Positive stainings for uPA, uPAR, and PAI‐1 were observed mainly in SCC cells, and their intensity and number of positive cells were related to lymph node involvement (p < 0.001, p < 0.001, and p < 0.001, respectively). The expression levels of uPA and uPAR were also related to the pattern of invasion (p < 0.05 and p < 0.001, respectively), while both were associated with tumor size (p < 0.05). Moreover, a poor survival rate was related to the expression of uPAR (p < 0.01) and PAI‐1 (p < 0.05). These findings suggest that the uPA system may regulate the invasion and metastasis of oral SCC cells.
Multilineage Cells from Apical Pulp of Human Tooth with Immature Apex
Authors: Abe Shigehiro , Yamaguchi Satoshi , Amagasa Teruo 
Published: August 2012

AbstractThe human tooth with immature apex is a developing organ available for investigation. In this tooth, especially in the apical pulp, the proliferation and differentiation of various cells are activated to make a complete tooth. We investigated the notion that unique cells are included in the apical pulp of human tooth with immature apex. Human impacted third molars with immature apex freshly extracted for orthodontic reasons or treatment were obtained. Histological analyses revealed that BrdU‐incorporating cells and cells positive for the mesenchymal stem cell markers SH2 and SH3 were located in the same region. The cells from the apical pulp of a human tooth with immature apex, designated here as apical pulp‐derived cells (APDCs), can be cultured easily in vitro under ordinary serum‐supplemented culture condition. The expression of surface markers of expanded APDCs is similar to that of bone marrow mesenchymal stem cells, except for CD49d (α4‐integrin). APDCs differentiated into mineralized cells, adipocytes, chondroblasts and neural cells in vitro. APDCs have a high capacity for proliferation and multilineage potential in vitro. Our results indicate that human tooth with immature apex is a precious tissue source for the research of human adult stem cells and for the advancement of dental and regenerative medicine.
Effects of Heparin‐binding Epidermal Growth Factor on the Invasion Activity of an Oral Cancer Cell Line
Authors: Furukawa Masayuki , Ohnishi Yuichi , Kakudo Kenji 
Published: August 2012

AbstractHeparin‐binding epidermal growth factor (EGF)‐like growth factor (HB‐EGF), which belongs to the EGF family, has been shown to stimulate the growth of a variety of cells in an autocrine or paracrine manner. Although HB‐EGF is widely expressed in tumors when compared to normal tissue, its contribution to tumor invasion is still not known. In the present study, we analyzed the effects of HB‐EGF on the invasion activity of a cultured oral cancer cell line using short interfering RNA (siRNA).Oral squamous carcinoma cell lines, HSC3 and SAS, were transfected with siRNA targeting HBEGF. Expression of HB‐EGF was analyzed by real‐time PCR. The invasiveness of the transfected cells was determined using a matrigel invasion assay, and MMP‐9 production was measured by RTPCR and gelatin zymography.The expression of HB‐EGF was reduced in HSC3‐siRNA and SAS‐siRNA cells. The matrigel invasion assay demonstrated that the invasiveness of HSC3‐siRNA and SAS‐siRNA cells was reduced. Gelatin zymography demonstrated that in HSC3‐siRNA and SAS‐siRNA cells, MMP9 production was decreased.These findings suggest that HB‐EGF expression is related to the invasion activity of oral cancer, particularly via regulation of MMP9.
Cystadenoma Arising from the Retromolar Region: A Case Report
Authors: Takahashi Yoshihiro , Kawano Kenji , Yanagisawa Shigetaka , Yokoyama Shigeo 
Published: August 2012

AbstractCystadenoma is an uncommon benign epithelial neoplasm. Cystadenoma usually occurs on the palatal and buccal mucosa, but is less commonly found in the retromolar regions.A 43‐year‐old male patient noticed a swelling on his gingiva distal to the right mandibular second molar. Physical examination showed a diffuse poorly circumscribed swelling extending from the lingual gingiva distal to the right mandibular second molar to the floor of the mouth and pharynx. The tumor size was 20 × 27 mm. The surface mucosa was purply‐blue in parts. About 4 ml of pale yellow serous fluid was aspirated. The tumor was surrounded by a capsule and was excised successfully above the periosteum, incorporating a 2 mm safety margin including the surface mucosa. The tumor consisted of a fibrous capsule with multiple cystic cavities of various sizes. The cysts were lined by a layer of columnar epithelium, with intracystic papillary proliferation in some areas. No cytologic atypia or invasion of the surrounding tissues were observed.There is no evidence of recurrence ten years after excision, suggesting that complete excision is sufficient treatment in such cases.
Benign Fibrous Histiocytoma of the Upper Lip: A Case Report
Authors: Toyohara Yukiyo , Hashitani Susumu , Sakurai Kazunari , Takaoka Kazuki , Urade Masahiro 
Published: August 2012

AbstractA rare case of benign fibrous histiocytoma arising in the upper lip in a 76‐year‐old female is presented. A well‐defined tumor measuring 1 cm existed in the submucosal area of the right side of the upper lip. With the patient under local anesthesia, the tumor was excised. Microscopically, the excised tumor was diagnosed as a benign fibrous histiocytoma. There has been no evidence of tumor recurrence after the operation.
Morphometric Analysis of Nuclear Features and Volume‐Corrected Mitotic Index in the Prognosis of Oral Squamous Cell Carcinoma
Authors: Natarajan Srikant , Mahajan Sumita , Boaz Karen , George Thomas 
Published: August 2012

AbstractIn the present study, an objective and reproducible evaluation of mitotic activity and nuclear morphometric factors was attempted in 30 patients of oral squamous cell carcinoma with a view to predicting local relapse and survival. Various nuclear parameters and volume‐corrected mitotic index were calculated and compared with the recurrence and death of the study group (n = 30). Volume‐corrected mitotic index (M/V index) was the single best prognosticator for recurrence of oral squamous cell carcinoma (p = 0.008 for recurrence; p = 0.015 for death). The combination of M/V and SD of nuclear area (forward stepwise regression) was a more efficient and better predictor of survival (Log rank test, Kaplan‐Meier’s survival analysis; χ2 = 17.46, p = 0.00001). The present study proved the effectiveness of the M/V index in predicting the biological behavior and the outcome of oral squamous cell carcinoma patients.
Preliminary Clinical Study to Evaluate the Relationship between Systemic Bone Turnover and the Microstructure of the Alveolar Bone
Authors: Arisaka Ryuichi , Matsuo Akira , Chiba Hiroshige , Takahashi Hidetoshi , Takeuchi Sawako , Watanabe Masato , Hojo Satoru 
Published: August 2012

AbstractThe objective of this study was to assess the possibility of developing a clinical minimally invasive and standardized method to evaluate the relationship between the microstructure of the jaw bone and systemic bone turnover. For this purpose, we performed standardized bone biopsy of the alveolar bone, and compared the 3D bone microstructure using micro‐computed tomography (micro‐CT) with bone mineral density (BMD) of the lumbar spine and biochemical markers of bone turnover. We evaluated a total of 9 samples taken from 6 patients by standardized biopsy using a trephine bur. BMD was evaluated using dual energy X‐ray absorptiometry (DXA). Regarding the biochemical markers of bone turnover, serum bone‐specific alkaline phosphatase (BAP) and serum osteocalcin (OC) were used as bone formation markers, and urinary cross‐linked N‐telopeptides of type I collagen (NTx) and urinary deoxypyridinoline (DPD) were selected as bone resorption markers. We scanned micro‐CT images of these samples. Bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular spacing (Tb.Spac), fractal dimension, trabecular bone pattern factor (TBPf) and node‐strut (Nd.Nd/TV, TSL/TV) were measured. Regarding the correlations between the parameters of bone microstructures, TB/TV, Tb.N, fractal dimension, and node‐strut seemed to be positively correlated and Tb.Spac and TBPf seemed to be negatively correlated with each other, but Tb.Th seemed to have a low correlation with other parameters. OC and/or BAP showed a significantly high correlation with many structural parameters (p < 0.05%). In conclusion, some microstructural parameters may change according to the systemic bone turnover.
Complications and Outcome of Free Flap Transfers for Oral and Maxillofacial Reconstruction: Analysis of 213 Cases
Authors: Yamashiro Masashi , Hasegawa Kazuki , Uzawa Narikazu , Michi Yasuyuki , Ishii Junichi , Yoshitake Hiroyuki , Kobayashi Junji , Yagihara Kazuhiro , Okabe Sadao , Amagasa Teruo 
Published: August 2012

AbstractMicrovascular free flap transfers have become a preferred reconstructive technique ; however, rare complications may still prove devastating. This study reviewed 213 consecutive freetissue transfers in order to assess the incidence and causes of complications in patients undergoing microvascular free flap reconstruction in the oral and maxillofacial region. In most cases, reconstruction was undertaken after resection of a malignant tumor. The flap donor sites were the radial forearm (n=111), rectus abdominis (n=88), scapula (n=13), and latissimus dorsi (n=1). The superior thyroid artery and the external jugular vein were commonly used as recipient vessels for anastomosis. The overall flap success rate was 99%. There were 7 cases of postoperative vascular thrombosis (6 venous and 1 arterial), constituting 3.3% of the entire series. Five flaps were salvaged, representing a 71.4% successful salvage rate in cases of vascular complications. Most of the successful salvage attempts were made within 24 hours of the end of the initial operation, and the successful salvage rate for re‐exploration was 100%. Finally, the total flap loss rate was 0.9% and the partial flap loss rate was 2.3%. We conclude that early re‐exploration should be the first choice for management of vascular compromised flaps. Complications at the donor site occurred in 17 cases (8.0%), the most common complication of which was partial skin graft loss after harvesting a radial forearm flap (n=10; 9.0%). Recipient and donor site morbidity was limited and considered acceptable.