bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood Pressure and Atrial Fibrillation: A Combined AF‐CHF and AFFIRM Analysis
Authors: TREMBLAY‐GRAVEL MAXIME , WHITE MICHEL , ROY DENIS , LEDUC HUGUES , WYSE D. GEORGE , CADRIN‐TOURIGNY JULIA , SHOHOUDI AZADEH , MACLE LAURENT , DUBUC MARC , ANDRADE JASON , RIVARD LENA , GUERRA PETER G. , THIBAULT BERNARD , TALAJIC MARIO , KHAIRY PAUL 
Published: February 2015

Blood Pressure and Atrial FibrillationBackgroundHypertension is an established risk factor for new‐onset atrial fibrillation (AF). However, the relationship between blood pressure and recurrent AF is less well understood.Methods and ResultsA pooled analysis of patient‐level data from AFFIRM and AF‐CHF trials was conducted on all 2,715 patients with paroxysmal or persistent AF, 68 ± 8 years, 66% male, randomized to rhythm control and followed for 40.6 ± 16.5 months. We assessed the impact of a baseline systolic blood pressure (SBP; <120 mmHg [N = 1,008], 120–140 mmHg [N = 930], >140 mmHg [N = 777]) on recurrent AF and proportion of time spent in AF. In patients with LVEF >40% (N = 1,719), SBP was not associated with recurrent AF in multivariate regression analyses (P = 0.752). In contrast, in patients with LVEF ≤40% (N = 996), the AF recurrence rate was higher in those with an SBP >140 mmHg compared to 120–140 mmHg (hazard ratio 1.47; 95% CI [1.12–1.93], P = 0.005). The rate of recurrent AF was similar in patients with SBP <120 mmHg compared to 120–140 mmHg (hazard ratio 1.15; 95% CI [0.92–1.43], P = 0.225). Consistently, the proportion of time spent in AF was not influenced by SBP in patients with LVEF >40% (P = 0.645). However, in patients with LVEF ≤40%, the adjusted mean proportion of time spent in AF was 17.2% if SBP was <120 mmHg, 15.4% for SBP 120–140 mmHg, and 24.0% for SBP >140 mmHg (P = 0.025).ConclusionSystolic blood pressure is an important determinant of recurrent AF and overall AF burden in patients with left ventricular dysfunction (LVEF≤40%) but not in those with preserved ventricular function.
Minimally Invasive Single Implant Treatment (M.I.S.I.T.) based on ridge preservation and contour augmentation in patients with a high aesthetic risk profile: one‐year results
Authors: Cosyn Jan , Pollaris Louis , Van der Linden Filip , De Bruyn Hugo 
Published: February 2015

AbstractAimTo evaluate the 1‐year outcome of Minimally Invasive Single Implant Treatment (M.I.S.I.T.) based on ridge preservation and contour augmentation in patients with a high aesthetic risk profile.Materials and MethodsPeriodontally healthy non‐smoking patients with a failing tooth in the anterior maxilla (15–25) were selected. All were in need of a single implant and demonstrated high risk for aesthetic complications given mid‐facial recession at the failing tooth and/or a buccal bone dehiscence and/or a thin‐scalloped gingival biotype. Patients without mid‐facial recession (NRG) received flapless tooth extraction and ridge preservation using a collagen‐enriched bovine‐derived xenograft, flapless installation of a bone condensing implant with variable‐thread design (4–6 months later), a provisional screw‐retained crown and connective tissue graft (CTG) inserted in the buccal mucosa (3 months later) and a permanent crown (3 months later). Patients with mid‐facial recession (RG) were treated similarly, yet they received a CTG at the time of ridge preservation. All patients were treated by an experienced periodontist using a microsurgical approach. Primary outcome variables included papillary and mid‐facial recession. Clinical parameters, pink (PES) and white aesthetic score (WES) were considered secondary outcome variables.ResultsFifty patients (25 females, 25 males; mean age 39, range 19–81; 42 in NRG and 8 in RG) met the selection criteria and consented to the treatment. Forty‐seven could be examined at 12 months and all implants survived. Mean bone loss amounted to 0.48 mm (range 0.00–1.80) at 12 months. Papillary recession was minimal in both groups at 12 months (mean ≤ 0.3 mm), as was mid‐facial recession in the NRG (mean 0.1 mm). Twelve patients in the NRG even demonstrated coronal migration of the mucosal margin following CTG and needed adaptation of the provisional crown to induce soft tissue retraction. Due to CTG at the time of ridge preservation in the RG, mid‐facial soft tissue gain amounted to 0.9 mm at 12 months, hereby eliminating 2/3rd of the initial recession. PES and WES were favourable pointing to 10.9/14 and 8.2/10 respectively.ConclusionThis short‐term prospective study offers a proof of principle of M.I.S.I.T. in patients with a high aesthetic risk profile.
Traps baited with pear ester and acetic acid attract both sexes of Hedya nubiferana (Lepidoptera: Tortricidae)
Authors: Jósvai J. K. , Koczor S. , Tóth M. 
Published: February 2015

AbstractThis study was initiated with the objective of studying field responses of the green budworm moth, Hedya nubiferana (Haworth) (Lepidoptera: Tortricidae), to pear ester [PE; ethyl (E,Z)‐2,4‐decadienoate] and acetic acid (AA) with the aim of developing a lure attractive also for females. In the overwhelming majority of tests, traps baited with the PEAA lure (the combination of both PE and AA) caught more than traps baited with either of the constituents presented alone. PEAA lures were attractive to H. nubiferana no matter whether the two compounds were provided in separate dispensers or mixed together in a single one, and a large percentage (up to 71%) of trap catch consisted of females. Traps with PEAA lures caught (females plus males) on an average 30% of the catches in traps baited with the synthetic green budworm moth sex pheromone (only males). This suggested that the new PEAA lure had a trapping performance comparable with that of pheromone traps, which latter are used by farmers today. Consequently, the PEAA lure showed potential for future practical applications as a female‐targeted lure for H. nubiferana. To our knowledge, this is the first well‐documented report on the attraction of PEAA lure for a tortricid species other than codling moth.
Action of anti‐M3 muscarinic acetylcholine receptor IgG of primary Sjögren's syndrome on the enzymatic antioxidant system in rat submandibular gland
Authors: Reina Silvia , Rodríguez Marcelo , Stranieri Graciela , Borda Enri 
Published: February 2015

BackgroundWe demonstrate that serum immunoglobulin G (IgG) directed against glandular M3 muscarinic acetylcholine receptors (M3 mAChR) and pilocarpine triggers the increment of superoxide dismutase (SOD) and catalase (CAT) and the production of nitric oxide (NO) and prostaglandin E2 (PGE2).MethodsEnzyme‐linked immunosorbent assay (ELISA) was performed in the presence of the human M3 mAChR synthetic peptide as antigen to detect in serum of pSS patients the autoantibodies. Further, SOD and CAT specific activity and NO were determined chemically in the presence of anti‐M3 mAChR IgG and pilocarpine. The level of PGE2 generation in the presence of autoantibody and pilocarpine was determined by ELISA.ResultsAn association between anti‐M3 mAChR autoantibodies and pilocarpine given the increment of the specific activity of SOD and CAT in the serum of pSS patients and in the rat submandibular gland was observed. As a result of this action, M3 synthetic peptide and atropine abrogated the stimulatory action. The L‐type calcium channel, calcium/calmodulin complex and COX‐2 inhibitors selectively blocked the increment of the specific activity of SOD and CAT in the rat submandibular gland. An increased production of NO and PGE2 by the cholinergic autoantibody and pilocarpine was also detected.ConclusionOn the basis of these results, the increment of the specific activity of SOD and CAT in pSS patients as compared to control healthy individuals may be seen as a defensive reaction to the increment of the amount of ROS, which becoming uncontrollable, leads to irreversible cellular and tissue damage.
Evidence of ancient genome reduction in red algae (Rhodophyta)
Authors: Qiu Huan , Price Dana C. , Yang Eun Chan , Yoon Hwan Su , Bhattacharya Debashish 
Published: February 2015

Red algae (Rhodophyta) comprise a monophyletic eukaryotic lineage of ~6,500 species with a fossil record that extends back 1.2 billion years. A surprising aspect of red algal evolution is that sequenced genomes encode a relatively limited gene inventory (~5–10 thousand genes) when compared with other free‐living algae or to other eukaryotes. This suggests that the common ancestor of red algae may have undergone extensive genome reduction, which can result from lineage specialization to a symbiotic or parasitic lifestyle or adaptation to an extreme or oligotrophic environment. We gathered genome and transcriptome data from a total of 14 red algal genera that represent the major branches of this phylum to study genome evolution in Rhodophyta. Analysis of orthologous gene gains and losses identifies two putative major phases of genome reduction: (i) in the stem lineage leading to all red algae resulting in the loss of major functions such as flagellae and basal bodies, the glycosyl‐phosphatidylinositol anchor biosynthesis pathway, and the autophagy regulation pathway; and (ii) in the common ancestor of the extremophilic Cyanidiophytina. Red algal genomes are also characterized by the recruitment of hundreds of bacterial genes through horizontal gene transfer that have taken on multiple functions in shared pathways and have replaced eukaryotic gene homologs. Our results suggest that Rhodophyta may trace their origin to a gene depauperate ancestor. Unlike plants, it appears that a limited gene inventory is sufficient to support the diversification of a major eukaryote lineage that possesses sophisticated multicellular reproductive structures and an elaborate triphasic sexual cycle.
Proteomic and gene expression analysis of zebrafish brain undergoing continuous light/dark stress
Authors: Purushothaman Sruthi , Saxena Sandeep , Meghah Vupalapathy , Meena Lakshmi Mula G. , Singh Sachin K. , Brahmendra Swamy Cherukuvada V. , Idris Mohammed M. 
Published: February 2015

SummarySeveral organisms irrespective of their complexity in structure and function have an inbuilt circadian rhythm. Zebrafish could be used as an alternate model animal in sleep research as it exhibits similar sleep–wake dynamics as mammals andDrosophila. In this study, we have analysed the adult zebrafish brain for its differential proteome and gene expression during perturbed light/dark cycle. A total of 53 and 25 proteins including sncb, peroxiredoxins andTCRalpha were identified based on two‐dimensional gel electrophoresis Fourier transform mass spectrometer/ion trap tandem mass spectrometer and differential in‐gel electrophoresisMALDI TOF MS/MSanalysis, respectively, with at least 1.5‐fold changes between the control and experimental brains. Real time‐polymerase chain reaction revealed that many circadian pathway‐associated genes, such as per1b, bmal1b, cry1b, bmal2 and nr1d2, were differentially regulated during continuous light/dark exposures. It is hypothesized that the differential regulation of these genes might lead to the discovery of potential diagnostic markers for gaining insight into the light/dark‐associated stress in humans.
Genetic isolation between two recently diverged populations of a symbiotic fungus
Authors: Branco Sara , Gladieux Pierre , Ellison Christopher E. , Kuo Alan , LaButti Kurt , Lipzen Anna , Grigoriev Igor V. , Liao Hui‐Ling , Vilgalys Rytas , Peay Kabir G. , Taylor John W. , Bruns Thomas D. 
Published: February 2015

AbstractFungi are an omnipresent and highly diverse group of organisms, making up a significant part of eukaryotic diversity. Little is currently known about the drivers of fungal population differentiation and subsequent divergence of species, particularly in symbiotic, mycorrhizal fungi. Here, we investigate the population structure and environmental adaptation in Suillus brevipes (Peck) Kuntze, a wind‐dispersed soil fungus that is symbiotic with pine trees. We assembled and annotated the reference genome for Su. brevipes and resequenced the whole genomes of 28 individuals from coastal and montane sites in California. We detected two clearly delineated coast and mountain populations with very low divergence. Genomic divergence was restricted to few regions, including a region of extreme divergence containing a gene encoding for a membrane Na+/H+ exchanger known for enhancing salt tolerance in plants and yeast. Our results are consistent with a very recent split between the montane and coastal Su. brevipes populations, with few small genomic regions under positive selection and a pattern of dispersal and/or establishment limitation. Furthermore, we identify a putatively adaptive gene that motivates further functional analyses to link genotypes and phenotypes and shed light on the genetic basis of adaptive traits.
The effects of music therapy in endotracheal suctioning of mechanically ventilated patients
Authors: Yaman Aktaş Yeşim , Karabulut Neziha 
Published: February 2015

ABSTRACTBackgroundEndotracheal suctioning has been identified as a painful procedure for critically ill patients.AimTo determine the effect of music therapy on pain intensity, sedation level and physiological parameters during endotracheal suctioning of mechanically ventilated patients in cardiovascular surgery intensive care unit (ICU).DesignExperimental survey.MethodsThe study was conducted between May 2010 and June 2013 in Ordu Medical Park Hospital Cardiovascular Surgery Intensive Care Unit. The study sample consisted of 66 patients (33 experimental and 33 control) who complied with the criteria of inclusion for the study. Data was collected using the ‘Patient Information Form’, ‘Critical‐Care Pain Observation Tool’, ‘Ramsay Sedation Scale’ and ‘Form of Physiological Parameters’.ResultsThe mean scores of the Ramsay Sedation Scale during endotracheal aspiration were respectively 1·88 and 1·55 in the experimental and control group and the difference between the groups was statistically significant (p = 0·003). The mean score of Critical‐Care Pain Observation Tool during endotracheal suctioning in the experimental group was found to be lower statistically than those of the control group (p < 0·001). There were no significant differences before, during and 20 min after suctioning between the two groups with regard to systolic blood pressure, diastolic blood pressure, heart rate and oxygen saturation (p > 0·05).ConclusionsThe results of this study implies that music therapy can be effective practice for nurses attempting to reduce patients' pain and control sedation level in patients on mechanical ventilators during endotracheal suctioning.Relevance to clinical practiceIt is recommended that music therapy should be added to the routine nursing care for mechanically ventilated patients.
The use of the MeDALL‐chip to assess IgE sensitization: a new diagnostic tool for allergic disease?
Authors: Skrindo Ingebjørg , Lupinek Christian , Valenta Rudolf , Hovland Vegard , Pahr Sandra , Baar Alexandra , Carlsen Kai‐Håkon , Mowinckel Petter , Wickman Magnus , Melen Erik , Bousquet Jean , Anto Josep M. , Lødrup Carlsen Karin C. 
Published: February 2015

AbstractBackgroundAllergic sensitization is frequently present in asthma and rhinitis, but the role of specific immunoglobulin E (s‐IgE) is not always clear. Multiple s‐IgE analyses may provide insight into this relationship, thus a microarray chip was developed within the EU‐funded MeDALL project. The main objective was to evaluate the performance of the MeDALL‐chip compared to ImmunoCAP and skin prick test (SPT) in detecting allergic sensitization in children and secondarily to investigate the association to asthma and allergic rhinitis.MethodsFrom the ‘Environment and Childhood Asthma Study’, 265 children were investigated at 10 and 16 yr of age with clinical examination, interview, SPT, ImmunoCAP, and the MeDALL‐chip including 152 allergen components in the analysis.ResultsAllergic sensitization at 10 yr was more frequently detected using the MeDALL‐chip (38.1%) compared to the ImmunoCAP (32.8%) (p = 0.034) and SPT (25.5%) (p < 0.001), but no significant difference was seen at 16 yr (MeDALL‐chip 49.8%, ImmunoCAP 48.6%, SPT 45.8%). The MeDALL‐chip did not differ significantly from the ImmunoCAP or SPT in terms of detecting allergic sensitization in subjects with rhinitis or asthma at 10 or 16 yr.ConclusionThe prevalence of allergic sensitization increased by all three diagnostic tests from 10 to 16 yr was similar by SPT and ImmunoCAP and significantly higher with the MeDALL‐chip at 10 yr. All three tests were comparable for identification of allergic sensitization among children with current rhinitis or asthma.
Freshwater occurrence of the extinct dolphinParapontoporia(Cetacea:Lipotidae) from the upperPliocene nonmarineTulareFormation ofCalifornia
Authors: Boessenecker Robert W. , Poust Ashley W. 
Published: February 2015

AbstractThe diminutive, extinct longirostrine dolphinParapontoporiais one of the most abundantly represented lateNeogene odontocetes from the easternNorthPacific and is widely known from numerous marine strata of lateMiocene andPliocene age inCalifornia,BajaCalifornia and possibly Japan.Parapontoporiahas been identified as the sister taxon of the recently extinctChinese river dolphin (Lipotes vexillifer); unlikeLipotes, which exclusively inhabited freshwater, the depositional context ofParapontoporiasuggests it was marine. A newly identified petrosal ofParapontoporiasp. was preserved alongside terrestrial vertebrates in the nonmarineTulareFormation (upperPliocene toPleistocene, 2.2–0.6 Ma),California, which was deposited under lacustrine and fluviodeltaic conditions. Abundantly preserved freshwater molluscs and rare marine taxa suggest predominantly freshwater settings with intermittent periods of estuarine conditions. This occurrence ofParapontoporiaindicates its presence in theSanJoaquin basin after the retreat of the inland sea and suggests that this extinct odontocete may have been freshwater tolerant and an inhabitant of marine and freshwater settings, heralding the exclusively freshwater existence of its Recent sister taxonLipotes vexillifer.
Biologic Response Modifiers and Pediatric Psoriasis
Authors: Bellodi Schmidt Fernanda , Shah Kara N. 
Published: February 2015

AbstractThe efficacy and safety of biologic response modifiers such as etanercept, adalimumab, infliximab, and ustekinumab have been demonstrated in the treatment of psoriasis in adults, but none are currently approved for the treatment of psoriasis in children in the United States, and only etanercept is approved for the treatment of psoriasis in children in the European Union. Through case reports, case series, and a large clinical trial of the use of etanercept, the literature supports the use of these agents to treat psoriasis in children. Data on the use of the tumor necrosis factor‐α antagonists etanercept, adalimumab, and infliximab in the treatment of other inflammatory diseases in children—namely Crohn's disease, juvenile arthritis, and uveitis—support their safety profile in children.
Lulwoana sp., a dark septate endophyte in roots of Posidonia oceanica (L.) Delile seagrass
Authors: Torta L. , Lo Piccolo S. , Piazza G. , Burruano S. , Colombo P. , Ottonello D. , Perrone R. , Di Maida G. , Pirrotta M. , Tomasello A. , Calvo S. 
Published: February 2015

AbstractPosidonia oceanica is the most common, widespread and important monocotyledon seagrass in the Mediterranean Basin, and hosts a large biodiversity of species, including microorganisms with key roles in the marine environment. In this study, we ascertain the presence of a fungal endophyte in the roots of P. oceanica growing on different substrata (rock, sand and matte) in two Sicilian marine meadows. Staining techniques on root fragments and sections, in combination with microscope observations, were used to visualise the fungal presence and determine the percentage of fungal colonisation (FC) in this tissue. In root fragments, statistical analysis of the FC showed a higher mean in roots anchored on rock than on matte and sand. In root sections, an inter‐ and intracellular septate mycelium, producing intracellular microsclerotia, was detected from the rhizodermis to the vascular cylinder. Using isolation techniques, we obtained, from both sampling sites, sterile, slow‐growing fungal colonies, dark in colour, with septate mycelium, belonging to the dark septate endophytes (DSEs). DNA sequencing of the internal transcribed spacer (ITS) region identified these colonies as Lulwoana sp. To our knowledge, this is the first report of Lulwoana sp. as DSE in roots of P. oceanica. Moreover, the highest fungal colonisation, detected in P. oceanica roots growing on rock, suggests that the presence of the DSE may help the host in several ways, particularly in capturing mineral nutrients through lytic activity.
Physical activity in patients with idiopathic pulmonary fibrosis
Authors: Nakayama Masayuki , Bando Masashi , Araki Koji , Sekine Toshie , Kurosaki Fumio , Sawata Tetsuro , Nakazawa Shoko , Mato Naoko , Yamasawa Hideaki , Sugiyama Yukihiko 
Published: February 2015

AbstractBackground and objectivePhysical activity is an important parameter in patients with chronic obstructive pulmonary disease, but has not been studied in detail in patients with interstitial lung disease. This study aimed to evaluate physical activity in patients with idiopathic pulmonary fibrosis (IPF).MethodsPhysical activity was monitored in 31 stable IPF patients using an accelerometer for 1 month. The following factors reflecting physical activity were measured: the number of steps, walking distance, the time spent at magnitude of movement (MM) 1–6, physical activity‐related energy expenditure (PAEE) and total energy expenditure. We also measured the following clinical parameters: the modified Medical Research Council (MRC) scale, Krebs von den Lungen‐6 (KL‐6), pulmonary function parameters, 6‐min walk test (6MWT) results and high‐resolution computed tomography (HRCT) findings of the chest. We determined the relationship between these parameters and physical activity.ResultsWe recorded 24 days of physical activity data. The time spent at MM < 1 was more than 10 h per day, whereas that at MM > 1 was approximately 1 h per day. The modified MRC scale, serum KL‐6 levels, 6MWT distance, and the extent of honeycomb and reticular abnormality on HRCT were associated with several facets of physical activity. In particular, lower KL‐6 levels were correlated with higher physical activity based on the number of steps, walking distance, the time spent at MM 1–4 and PAEE.ConclusionsThe modified MRC scale, 6MWT distance, extent of fibrosis on HRCT and serum KL‐6 levels are strongly associated with physical activity.