bluuebunny/crossref_metadata_2025_split huggingface dataset:
The headache research priorities: Research goals from the American Headache Society and an international multistakeholder expert group
Authors: Schwedt Todd J. , Pradhan Amynah A. , Oshinsky Michael L. , Brin Mitchell F. , Rosen Howard , Lalvani Nim , Charles Andrew , Ashina Messoud , Do Thien Phu , Burstein Rami , Gelfand Amy A. , Dodick David W. , Pozo‐Rosich Patricia , Lipton Richard B. , Ailani Jessica , Szperka Christina L. , Charleston Larry , Digre Kathleen B. , Russo Andrew F. , Buse Dawn C. , Powers Scott W. , Tassorelli Cristina , Goadsby Peter J. ,   the Headache Research Priorities Working Group Members
Published: August 2024

AbstractObjectiveTo identify and disseminate research priorities for the headache field that should be areas of research focus during the next 10 years.BackgroundEstablishing research priorities helps focus and synergize the work of headache investigators, allowing them to reach the most important research goals more efficiently and completely.MethodsThe Headache Research Priorities organizing and executive committees and working group chairs led a multistakeholder and international group of experts to develop headache research priorities. The research priorities were developed and reviewed by clinicians, scientists, people with headache, representatives from headache organizations, health‐care industry representatives, and the public. Priorities were revised and finalized after receiving feedback from members of the research priorities working groups and after a public comment period.ResultsTwenty‐five research priorities across eight categories were identified: human models, animal models, pathophysiology, diagnosis and management, treatment, inequities and disparities, research workforce development, and quality of life. The priorities address research models and methods, development and optimization of outcome measures and endpoints, pain and non‐pain symptoms of primary and secondary headaches, investigations into mechanisms underlying headache attacks and chronification of headache disorders, treatment optimization, research workforce recruitment, development, expansion, and support, and inequities and disparities in the headache field. The priorities are focused enough that they help to guide headache research and broad enough that they are widely applicable to multiple headache types and various research methods.ConclusionsThese research priorities serve as guidance for headache investigators when planning their research studies and as benchmarks by which the headache field can measure its progress over time. These priorities will need updating as research goals are met and new priorities arise.
Improved precision and mechanical properties of 3D‐printed silica ceramics via sintering temperature optimization
Authors: Mu Yunsong , Chen Yanhua , Li He , Sun Jingwen , Mu Baoxia , Colombo Paolo 
Published: August 2024

AbstractCeramic cores are the key components of precision casting hollow turbine blades, and 3D‐printed silica‐based ceramic cores are crucial to the development of the aerospace industry. However, silica‐based ceramic cores have problems in terms of mechanical properties and friction properties. In this paper, silica ceramics were prepared by stereolithography‐based 3D printing technology and processed at different sintering temperatures. The effect of sintering temperature on the microstructure, physical–mechanical properties, and friction and wear properties of the silica ceramics was investigated. The results show that, with the increase of sintering temperature, the average particle size and bulk density of the samples increased, while the open porosity and layer thickness decreased. The surface of ceramics became more and more flat with the increase in temperature. The flexural strength first increased with increasing temperature, and then suddenly decreased at 1350°C. The average surface roughness decreased with increasing temperature. The wear of the material decreased with increasing sintering temperature and increased at 1350°C. The optimum sintering temperatures were 1250°C and 1300°C, giving a flexural strength of 23.18 and 23.25 MPa, bulk density of 1.72 and 1.78 g/cm3, and open porosity of 24.49% and 23.66%, respectively.
Bombyx mori nucleopolyhedrovirus LEF‐2 disrupts the cell cycle in the G2/M phase by triggering a host cell DNA damage response
Authors: Wang Jie , Xiao Miao , Hu Zhigang , Lin Yu , Li Kejie , Chen Peng , Lu Cheng , Dong Zhanqi , Pan Minhui 
Published: August 2024

AbstractIt is a common strategy for viruses to block the host cell cycle to favour their DNA replication. Baculovirus, being a double‐stranded DNA virus, can arrest the cell cycle in the G2/M phase to facilitate its replication. However, the key viral genes and mechanisms crucial for inducing cell cycle arrest remain poorly understood. Here, we initially examined the impacts of several Bombyx mori nucleopolyhedrovirus (BmNPV) DNA replication‐associated genes: ie1, lef‐1, lef‐2, lef‐3, lef‐4, odv‐ec27 and dbp. We assessed their effects on both the host cells' DNA replication and cell cycle. Our findings reveal that when the lef‐2 gene was overexpressed, it led to a significant increase in the number of cells in the G2/M phase and a reduction in the number of cells in the S phase. Furthermore, we discovered that the LEF‐2 protein is located in the virogenic stroma and confirmed its involvement in viral DNA replication. Additionally, by employing interference and overexpression experiments, we found that LEF‐2 influences host cell DNA replication and blocks the cell cycle in the G2/M phase by regulating the expression of CyclinB and CDK1. Finally, we found that BmNPV lef‐2 triggered a DNA damage response in the host cell, and inhibiting this response removed the cell cycle block caused by BmNPV LEF‐2. Thus, our findings indicate that the BmNPV lef‐2 gene plays a crucial role in viral DNA replication and can regulate host cell cycle processes. This study furthers our understanding of baculovirus‐host cell interactions and provides new insight into the molecular mechanisms of antiviral research.
Disaster preparedness perceptions and psychological first‐aid competencies of psychiatric nurses
Authors: Çömez Ikican Tuba , Şahin Bayindir Gizem , Engin Yusuf , Albal Esra 
Published: August 2024

AbstractAimThis study aimed to determine the disaster preparedness perceptions and psychological first‐aid competencies of psychiatric nurses.BackgroundNurses must recognize the risks before a disaster occurs and have an action plan for providing effective physical and psychological care to patients and other affected individuals during and after the disaster. It is especially important for psychiatric nurses caring for a high‐risk group, such as psychiatric patients, to be prepared for disasters and be able to support patients by recognizing the psychological reactions that may occur after a disaster and managing their care accordingly.MethodsFor this cross‐sectional and correlational study, data on 122 nurses were collected using a personal information form, the preparedness perception scale of disaster in nurses, and the psychological first‐aid application self‐efficacy scale.ResultsThe mean score for the participants’ perception of overall disaster preparedness was 76.70 ± 8.30, the mean score for the preparation stage was 27.02 ± 2.82, the mean score for the intervention stage was 30.40 ± 5.15, the mean score for the post‐disaster stage was 19.27 ± 2.88, and the mean score for psychological first‐aid application self‐efficacy was 140.28 ± 19.17.ConclusionThe findings showed that psychiatric nurses were above the medium level in terms of disaster preparedness perceptions and psychological first‐aid application self‐efficacy.Implications for nursing and health policyIt is important for nurses caring for high‐risk groups, such as psychiatric patients, to be aware of disaster preparedness, to recognize psychological reactions that may be observed after a disaster, and to be aware of psychological first‐aid practices that can be used to intervene in a disaster.
Association of Hepatitis B Surface Antigen Levels With Long‐Term Complications in Chronic Hepatitis B Virus Infection: A Systematic Literature Review
Authors: Mahajan Anadi , Kharawala Saifuddin , Desai Supriya , Kendrick Stuart , Das Joyeta , Gielen Vera 
Published: August 2024

ABSTRACTChronic hepatitis B virus (HBV) infection is a global issue and can lead to cirrhosis and hepatocellular carcinoma (HCC). Hepatitis B surface antigen (HBsAg) is an important marker of HBV infection and HBsAg quantification could be a useful tool in clinical practice. This systematic literature review aimed to explore the association between HBsAg titres and long‐term disease outcomes and evaluate the relationship between HBsAg titres, or changes in HBsAg titres, and clinical and treatment characteristics in patients with chronic HBV infection. Structured searches were performed in MEDLINE and Embase (January 2000 to 31 March 2023). Eighty‐two studies were included, comprising 51% retrospective cohort studies, mostly conducted in Asia (85%). HBsAg levels were shown to predict the long‐term development of cirrhosis and HCC in patients who were untreated prior to and during follow‐up; however, these data were inconclusive in mixed and treated populations. HBsAg titres were significantly associated with various virological markers including serum HBV DNA, HBcrAg, HBeAg, HBV RNA levels, intrahepatic covalently closed circular DNA (cccDNA) and intrahepatic HBsAg expression. HBsAg titres generally declined over time; this decline was more pronounced in early (HBeAg‐positive) than later disease phases (HBeAg‐negative). Higher decline in HBsAg levels was consistently associated with subsequent HBsAg seroclearance and a greater decline in total intrahepatic HBV DNA and cccDNA levels. In conclusion, this review showed that HBsAg levels and rates of decline could inform assessment, management and prediction of outcomes in chronic HBV infection. Further studies in broader, more diverse populations and treated patients are needed.
Internet‐delivered emotion regulation therapy for adolescents engaging in non‐suicidal self‐injury and their parents: A qualitative, online focus group study
Authors: Christensen Sofie Heidenheim , Heinrichsen Michella , Møhl Bo , Rubæk Lotte , Byrialsen Katherine Krage , Ojala Olivia , Hellner Clara , Pagsberg Anne Katrine , Bjureberg Johan , Morthorst Britt 
Published: August 2024

AbstractObjectivesWe explore adolescents' and their parents' experiences of internet‐based emotion regulation therapy for non‐suicidal self‐injury (NSSI).DesignA qualitative study nested within a controlled feasibility trial.MethodsOnline, semi‐structured focus group interviews were conducted with outpatient adolescents with NSSI aged 13–17 years (n = 9) and their parents (n = 8) who had received therapist‐guided Internet‐delivered Emotion Regulation Individual Therapy for Adolescents (IERITA). Transcripts were analysed using reflexive thematic analysis.ResultsThree main themes were generated: (1) Fatigue – barriers to and during treatment, comprised of two sub‐themes ‘Arriving to services exhausted, needing motivation, and leaving feeling abandoned’ and ‘the burden of IERITA and the consequences of fatigue’, (2) inter‐ and intrapersonal insights as facilitators of change and (3) Online, written contact with the therapist is beneficial and contributes with less pressure, comprised of three sub‐themes ‘the therapist behind the screen is essential’, ‘less pressure sitting alone: the physical absence of a therapist’ and ‘engaging on your own terms, in your own tempo’. Themes were consistent among adolescents and parents.ConclusionFatigue due to therapeutic engagement and previous help‐seeking processes created barriers for engagement. Emotion regulation therapy was experienced as beneficial leading to inter‐ and intra‐personal insights, facilitating change of maladaptive patterns. Therapists were regarded as indispensable, and the internet‐based format did not hinder therapeutic alliance. The written format allowed for reflection and alleviated the pressure of relating to the therapist. Further research should explore experiences of other online treatment formats (e.g. synchronous or video‐based) with regard to benefits, fatigue and therapist interaction.
The role of melatonin in delaying senescence and maintaining quality in postharvest horticultural products
Authors: Liu Y. , Xu J. , Lu X. , Huang M. , Yu W. , Li C. 
Published: August 2024

AbstractThe postharvest lifespan of horticultural products is closely related to loss of nutritional quality, accompanied by a rapid decline in shelf life, commercial value, and marketability. Melatonin (MT) application not only maintains quality but also delays senescence in horticultural products. This paper reviews biosynthesis and metabolism of endogenous MT, summarizes significant effects of exogenous MT application on postharvest horticultural products, examines regulatory mechanisms of MT‐mediated effects, and provides an integrated review for understanding the positive role of MT in senescence delay and quality maintenance. As a multifunctional molecule, MT coordinates other signal molecules, such as ABA, ETH, JA, SA, NO, and Ca2+, to regulate postharvest ripening and senescence. Several metabolic pathways are involved in regulation of MT during postharvest senescence, including synthesis and signal transduction of plant hormones, redox homeostasis, energy metabolism, carbohydrate metabolism, and degradation of pigment and cell wall components. Moreover, MT regulates expression of genes related to plant hormones, antioxidant systems, energy generation, fruit firmness and colour, membrane integrity, and carbohydrate storage. Consequently, MT could become an emerging and eco‐friendly preservative to extend shelf life and maintain postharvest quality of horticultural products.
Evaluating preoperative coagulation panels in dogs undergoing liver lobectomy for primary liver tumors: A multi‐institutional retrospective study
Authors: Burkhardt Samuel J. , Drobatz Kenneth L. , Callan Beth , Culp William T. N. , Selmic Laura E. , Tobias Karen , Wallace Mandy L. , Worley Deanna , Holt David E. 
Published: August 2024

AbstractBackgroundThe objectives of this study were to: (i) Determine whether operable primary liver tumors were associated with prolongations in prothrombin time (PT) and activated partial thromboplastin time (aPTT) and (ii) determine if these secondary hemostatic abnormalities were more prevalent with specific liver tumors.Study designMulti‐institutional retrospective study.Animal populationDogs (n = 359) undergoing liver lobectomy for a primary liver tumor with a preoperative coagulation panel.MethodsData was identified via electronic medical record review at eight veterinary teaching hospitals. Baseline dog characteristics, coagulation panel values, platelet count, emergency versus non‐emergency procedure, whether the dogs received transfusion(s) of a blood product, liver lobe removed, and histopathological diagnosis were extracted from the medical record. Chi‐square analysis was used to compare categorical variables between groups. Continuous variables were assessed for normality using the Shapiro–Wilk test.ResultsA total of 74 of 359 dogs (20.6%) had a prolongation in either PT or aPTT preoperatively. A total of 20 of 359 dogs (5.6%) were found to have prolongation of both PT and aPTT. Hemangiosarcoma was the only histopathological diagnosis associated with concurrent prolongations of both PT and aPTT (p < .001) in 6/16 (37.5%) dogs.ConclusionCoagulation panels including PT and aPTT are unlikely to detect substantial deficiencies in secondary hemostasis in most dogs with primary liver tumors except in dogs with a histopathological diagnosis of hemangiosarcoma.Clinical significancePT and aPTT testing is low yield as an elective preoperative screening test in dogs with primary liver tumors except in dogs where there is a hemoabdomen or high suspicion for hepatic hemangiosarcoma.
Conservation genetics of barbel species (Teleostei, Cyprinidae) facing hybridization and introgression along an elevational gradient in protected areas of northern Italy
Authors: Ferrari Claudio , Piccoli Federica , Voccia Andrea , Rontani Pietro Maria , Leonardi Stefano , Ardenghi Alessia , Nonnis Marzano Francesco , Filonzi Laura 
Published: August 2024

AbstractThe European endemic barbels represent important bioindicators of river quality and are nowadays threatened by changing environmental conditions and hybridization with the invasive alien Barbus barbus. It is therefore fundamental to investigate interactions among species and adaptability to climate changes in protected areas of Northern Apennines. An investigation was carried out considering 248 barbel samples that were analysed for Cytb mitochondrial DNA and 192 at 10 microsatellite loci, to delineate the distribution and population structure of the two native species as well as the impact of invasive B. barbus inside 15 sites of the Natura 2000 network. The complex distribution of the native barbel species was highlighted, together with a significant genetic structure emerging in different populations. Only one site revealed a “pure” population of B. caninus while the other ones showed a high level of hybridization between the different barbel species. For the B. plebejus two “genetically pure” populations were found in the hill‐mountain sector, while the hybridization level resulted in increasing in the lowest altitudinal stretch of watercourses with a consistent contribution driven by B. barbus. We herein present the first evidence of B. barbus introgression along an altitudinal gradient, carried from the lowland water course to mountain stretches driven by B. plebejus migration. B. plebejus is the species that can act as vicariant organism able to transfer the B. barbus genome from the plain habitats into B. caninus genome of the higher altitude waterstreams, as a consequence of habitat shifts due to climate changes and anthropogenic acitivities.
From raw milk cheese to the gut: investigating the colonization strategies of
Bifidobacterium mongoliense
Authors: Longhi Giulia , Lugli Gabriele Andrea , Tarracchini Chiara , Fontana Federico , Bianchi Massimiliano Giovanni , Carli Elisa , Bussolati Ovidio , van Sinderen Douwe , Turroni Francesca , Ventura Marco 
Published: August 2024

ABSTRACT

The microbial ecology of raw milk cheeses is determined by bacteria originating from milk and milk-producing animals. Recently, it has been shown that members of the
Bifidobacterium mongoliense
species may become transmitted along the Parmigiano Reggiano cheese production chain and ultimately may colonize the consumer intestine. However, there is a lack of knowledge regarding the molecular mechanisms that mediate the interaction between
B. mongoliense
and the human gut. Based on 128 raw milk cheeses collected from different Italian regions, we isolated and characterized 10
B. mongoliense
strains. Comparative genomics allowed us to unveil the presence of enzymes required for the degradation of sialylated host-glycans in
B. mongoliense
, corroborating the appreciable growth on de Man-Rogosa-Sharpe (MRS) medium supplemented with 3’-sialyllactose (3′-SL) or 6’-sialyllactose (6′-SL). The
B. mongoliense
BMONG18 was chosen, due to its superior ability to utilize 3′-SL and mucin as representative strain, to investigate its behavior when co-inoculated with other bifidobacterial species. Conversely, members of other bifidobacterial species did not appear to benefit from the presence of BMONG18, highlighting a competitive scenario for nutrient acquisition. Transcriptomic data of BMONG18 reveal no significant differences in gene expression when cultivated in a gut simulating medium (GSM), regardless of whether cheese was included or not. Furthermore, BMONG18 was shown to exhibit high adhesion capabilities to HT29-MTX human cells, in line with its colonization ability of a human host.

IMPORTANCE

Fermented foods are nourishments produced through controlled microbial growth that play an essential role in worldwide human nutrition. Research interest in fermented foods has increased since the 80s, driven by growing awareness of their potential health benefits beyond mere nutritional content.
Bifidobacterium mongoliense
, previously identified throughout the production process of Parmigiano Reggiano cheese, was found to be capable of establishing itself in the intestines of its consumers. Our study underscores molecular mechanisms through which this bifidobacterial species, derived from food, interacts with the host and other gut microbiota members.
Predicting
Pseudomonas aeruginosa
drug resistance using artificial intelligence and clinical MALDI-TOF mass spectra
Authors: Nguyen Hoai-An , Peleg Anton Y. , Song Jiangning , Antony Bhavna , Webb Geoffrey I. , Wisniewski Jessica A. , Blakeway Luke V. , Badoordeen Gnei Z. , Theegala Ravali , Zisis Helen , Dowe David L. , Macesic Nenad 
Published: August 2024

ABSTRACT

Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) is widely used in clinical microbiology laboratories for bacterial identification but its use for detection of antimicrobial resistance (AMR) remains limited. Here, we used MALDI-TOF MS with artificial intelligence (AI) approaches to successfully predict AMR in
Pseudomonas aeruginosa
, a priority pathogen with complex AMR mechanisms. The highest performance was achieved for modern β-lactam/β-lactamase inhibitor drugs, namely, ceftazidime/avibactam and ceftolozane/tazobactam. For these drugs, the model demonstrated area under the receiver operating characteristic curve (AUROC) of 0.869 and 0.856, specificity of 0.925 and 0.897, and sensitivity of 0.731 and 0.714, respectively. As part of this work, we developed dynamic binning, a feature engineering technique that effectively reduces the high-dimensional feature set and has wide-ranging applicability to MALDI-TOF MS data. Compared to conventional feature engineering approaches, the dynamic binning method yielded highest performance in 7 of 10 antimicrobials. Moreover, we showcased the efficacy of transfer learning in enhancing the AUROC performance for 8 of 11 antimicrobials. By assessing the contribution of features to the model's prediction, we identified proteins that may contribute to AMR mechanisms. Our findings demonstrate the potential of combining AI with MALDI-TOF MS as a rapid AMR diagnostic tool for
Pseudomonas aeruginosa
.

IMPORTANCE

Pseudomonas aeruginosa
is a key bacterial pathogen that causes significant global morbidity and mortality. Antimicrobial resistance (AMR) emerges rapidly in
P. aeruginosa
and is driven by complex mechanisms. Drug-resistant
P. aeruginosa
is a major challenge in clinical settings due to limited treatment options. Early detection of AMR can guide antibiotic choices, improve patient outcomes, and avoid unnecessary antibiotic use. Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) is widely used for rapid species identification in clinical microbiology. In this study, we repurposed mass spectra generated by MALDI-TOF and used them as inputs for artificial intelligence approaches to successfully predict AMR in
P. aeruginosa
for multiple key antibiotic classes. This work represents an important advance toward using MALDI-TOF as a rapid AMR diagnostic for
P. aeruginosa
in clinical settings.
Cyanothioacetamide in Multicomponent Synthesis of 2-Thionicotinonitrile Derivatives
Authors: Dyachenko I. V. , Dyachenko V. D. , Dorovatovskiy P. V. , Khrustalev V. N. , Nenaidenko V. G. 
Published: August 2024

Abstract
Multicomponent condensation of cyanothioacetamide, carbonyl compounds, enamines and alkylating reagents initiated by the Knoevenagel reaction led to the formation of 2-thionicotinonitriles. The molecular and crystal structure of the prepared products was studied by single crystal X-ray diffraction method.