bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adeno‐associated virus serotype 9 administered systemically after reperfusion preferentially targets cardiomyocytes in the infarct border zone with pharmacodynamics suitable for the attenuation of left ventricular remodeling
Authors: Konkalmatt Prasad R. , Wang Feng , Piras Bryan A. , Xu Yaqin , O'Connor Daniel M. , Beyers Ronald J. , Epstein Frederick H. , Annex Brian H. , Hossack John A. , French Brent A. 
Published: October 2012

AbstractBackgroundAdeno‐associated virus serotype 9 (AAV9) vectors provide efficient and uniform gene expression to normal myocardium following systemic administration, with kinetics that approach steady‐state within 2–3 weeks. However, as a result of the delayed onset of gene expression, AAV vectors have not previously been administered intravenously after reperfusion for post‐infarct gene therapy applications. The present study evaluated the therapeutic potential of post‐myocardial infarction gene delivery using intravenous AAV9.MethodsAAV9 vectors expressing firefly luciferase, enhanced green fluorescent protein (eGFP) or extracellular superoxide dismutase genes from the cardiac troponin‐T (cTnT) promoter (AcTnTLuc, AcTnTeGFP, AcTnTEcSOD) were employed. AcTnTLuc was administered intravenously at 10 min and at 1, 2 and 3 days post‐ischemia/reperfusion (IR), and the kinetics of luciferase expression were assessed with bioluminescence imaging. AcTnTeGFP was used to evaluate the distribution of eGFP expression. High‐resolution echocardiography was used to evaluate the effects of AcTnTEcSOD on left ventricular (LV) remodeling when injected 10 min post‐IR.ResultsCompared to sham animals, luciferase expression at 2 days after vector administration was elevated by four‐, 24‐, 210‐ and 213‐fold in groups injected at 10 min, 1 day, 2 days and 3 days post‐IR, respectively. The expression of cTnT‐driven eGFP was strongest in cardiomyocytes bordering the infarct zone. In the efficacy study of EcSOD, post‐infarct LV end‐systolic and end‐diastolic volumes at days 14 and 28 were significantly smaller in the EcSOD group compared to the control.ConclusionsSystemic administration of AAV9 vectors after IR both elevates and accelerates gene expression that preferentially targets cardiomyocytes in the border zone with pharmacodynamics suitable for the attenuation of LV remodeling. Copyright © 2012 John Wiley & Sons, Ltd.
Nutrition in the hospitalized patient
Authors: Kirkland Lisa L. , Kashiwagi Deanne T. , Brantley Susan , Scheurer Danielle , Varkey Prathibha 
Published: October 2012

AbstractAlmost 50% of patients are malnourished on admission; many others develop malnutrition during admission. Malnutrition contributes to hospital morbidity, mortality, costs, and readmissions. The Joint Commission requires malnutrition risk screening on admission. If screening identifies malnutrition risk, a nutrition assessment is required to create a nutrition care plan. The plan should be initiated early in the hospital course, as even patients with normal nutrition become malnourished quickly when acutely ill. While the Harris‐Benedict equation is the most commonly used method to estimate calories, its accuracy may not be optimal in all patients. Calculating the caloric needs of acutely ill obese patients is particularly problematic. In general, a patient's caloric intake should be slightly less than calculated needs to avoid the metabolic risks of overfeeding. However, most patients do not receive their goal calories or receive parenteral nutrition due to erroneous practices of awaiting return of bowel sounds or holding feeding for gastric residual volumes. Patients with inadequate intake over time may develop potentially fatal refeeding syndrome. The hospitalist must be able to recognize the risk factors for malnutrition, patients at risk of refeeding syndrome, and the optimal route for nutrition support. Finally, education of patients and their caregivers about nutrition support must begin before discharge, and include coordination of care with outpatient facilities. As with all other aspects of discharge, it is the hospitalist's role to assure smooth transition of the nutrition care plan to an outpatient setting. Journal of Hospital Medicine 2013. © 2012 Society of Hospital Medicine
Prospective comparison of curbside versus formal consultations
Authors: Burden Marisha , Sarcone Ellen , Keniston Angela , Statland Barbara , Taub Julie A. , Allyn Rebecca L. , Reid Mark B. , Cervantes Lilia , Frank Maria G. , Scaletta Nicholas , Fung Philip , Chadaga Smitha R. , Mastalerz Katarzyna , Maller Nancy , Mascolo Margherita , Zoucha Jeff , Campbell Jessica , Maher Mary P. , Stella Sarah A. , Albert Richard K. 
Published: October 2012

AbstractBACKGROUND:Curbside consultations are commonly requested during the care of hospitalized patients, but physicians perceive that the recommendations provided may be based on inaccurate or incomplete information.OBJECTIVE:To compare the accuracy and completeness of the information received from providers requesting a curbside consultation of hospitalists with that obtained in a formal consultation on the same patients, and to examine whether the recommendations offered in the 2 consultations differed.DESIGN:Prospective cohort.SETTING:University‐affiliated, urban safety net hospital.MAIN OUTCOME MEASURES:Proportion of curbside consultations with inaccurate or incomplete information; frequency with which recommendations in the formal consultation differed from those in the curbside consultation.RESULTS:Curbside consultations were requested for 50 patients, 47 of which were also evaluated in a formal consultation performed on the same day by a hospitalist other than the one performing the curbside consultation. Based on information collected in the formal consultation, information was either inaccurate or incomplete in 24/47 (51%) of the curbside consultations. Management advice after formal consultation differed from that given in the curbside consultation for 28/47 patients (60%). When inaccurate or incomplete information was received, the advice provided in the formal versus the curbside consultation differed in 22/24 patients (92%, P < 0.0001).CONCLUSIONS:Information presented during inpatient curbside consultations of hospitalists is often inaccurate or incomplete, and this often results in inaccurate management advice. Journal of Hospital Medicine 2013. © 2012 Society of Hospital Medicine
Neuroanatomy of halobiotus crispae (eutardigrada: hypsibiidae): Tardigrade brain structure supports the clade panarthropoda
Authors: Persson Dennis K. , Halberg Kenneth A. , Jørgensen Aslak , Møbjerg Nadja , Kristensen Reinhardt M. 
Published: October 2012

AbstractThe position of Tardigrada in the animal tree of life is a subject that has received much attention, but still remains controversial. Whereas some think tardigrades should be categorized as cycloneuralians, most authors argue in favor of a phylogenetic position within Panarthropoda as a sister group to Arthropoda or Arthropoda + Onychophora. Thus far, neither molecular nor morphological investigations have provided conclusive results as to the tardigrade sister group relationships. In this article, we present a detailed description of the nervous system of the eutardigrade Halobiotus crispae, using immunostainings, confocal laser scanning microscopy, and computer‐aided three‐dimensional reconstructions supported by transmission electron microscopy. We report details regarding the structure of the brain as well as the ganglia of the ventral nerve cord. In contrast to the newest investigation, we find transverse commissures in the ventral ganglia, and our data suggest that the brain is partitioned into at least three lobes. Additionally, we can confirm the existence of a subpharyngeal ganglion previously called subesophagal ganglion. According to our results, the original suggestion of a brain comprised of at least three parts cannot be rejected, and the data presented supports a sister group relationship of Tardigrada to 1) Arthropoda or 2) Onychophora or 3) Arthropoda + Onychophora. J. Morphol. 2012. © 2012 Wiley Periodicals, Inc.
Customer‐based Brand Equity for a Destination: the Effect of Destination Image on Preference for Products Associated with a Destination Brand
Authors: Lim Yumi , Weaver Pamela A. 
Published: October 2012

ABSTRACTThis study investigated how destination image of customer‐based brand equity for a tourist destination can be transferred to products associated with a destination brand. The effect of destination image on preference for products associated with a destination brand (PPADB) and the relationship between tourists’ destination preference and PPADB were investigated. Two hundred and ninety six individuals replied to a self‐administered questionnaire distributed to 318 students at a major US university. The results revealed that both cognitive and affective image of a destination are positively related to PPADB; preference for a destination is also positively related to PPADB. Suggestions are provided for a successful destination brand extension strategy. Copyright © 2012 John Wiley & Sons, Ltd.
Ternary Donor–Insulator–Acceptor Systems for Polymer Solar Cells
Authors: Li Sijun , Lu Guanghao , Li Hui , Qu Yunpeng , Li Ligui , Loos Joachim , Yang Xiaoniu 
Published: October 2012

AbstractA series of block copolymers with fixed length of the semiconductor‐block poly(3‐butylthiophene) (P3BT) and varying length of the insulator‐block polystyrene (PS) are synthesized. These copolymers are blended with phenyl‐C61‐butyric acid methyl ester (PCBM) for the bulk heterojunction photoactive layers. With appropriate insulator‐block length and donor–acceptor ratio, the power conversion efficiency increases by one order of magnitude compared with reference devices with pure P3BT/PCBM. PS blocks improve the miscibility of the active layer blends remarkably. The P3BT‐b‐PS crystallizes as nanorods with the P3BT core covered with the PS‐block, which creates a nanoscale tunneling barrier between donor and acceptor leading to more efficient transportation of charge carriers in the semiconductors.
Cell(MC3T3‐E1)‐Printed Poly(ϵ‐caprolactone)/Alginate Hybrid Scaffolds for Tissue Regeneration
Authors: Lee HyeongJin , Ahn SeungHyun , Bonassar Lawrence J. , Kim GeunHyung 
Published: October 2012

AbstractA new cell‐printed scaffold consisting of poly(ϵ‐caprolactone) (PCL) and cell‐embedded alginate struts is designed. The PCL and alginate struts are stacked in an interdigitated pattern in successive layers to acquire a three‐dimensional (3D) shape. The hybrid scaffold exhibits a two‐phase structure consisting of cell (MC3T3‐E1)‐laden alginate struts able to support biological activity and PCL struts able to provide controllable mechanical support of the cell‐laden alginate struts. The hybrid scaffolds exhibit an impressive increase in tensile modulus and maximum strength compared to pure alginate scaffolds. Laden cells are homogeneously distributed throughout the alginate struts and the entire scaffold, resulting in cell viability of approximately 84%.
Synthesis and Photophysical Properties of Polyfluorene With Dipicolylamine Groups on the Side Chain: Highly Selective and Sensitive Detection of Histidine
Authors: Zhang Wenjun , Qin Jingui , Yang Chuluo 
Published: October 2012

AbstractTwo new polyfluorenes with dipicolylamine (DPA) pendant, PF‐TDPA and PF‐HDPA, are designed and synthesized by pre‐ and post‐functionalization, respectively. PF‐TDPA with a rigid side chain shows a selective fluorescence quenching upon the addition of Cu2+ in a mixture solution of tetrahydrofuran and 4‐(2‐hydroxyethyl)‐1‐piperazineethanesulfonic acid (HEPES) buffer. What is more, the PF‐TDPA/Cu2+ complex can selectively detect histidine over other amino acids with a fluorescence recovery. In contrast, PF‐HDPA with a flexible spacer exhibits a fluorescence quenching to Cu2+ but slightly fluorescence recovery after the addition of histidine. This indicates that the proper distance between the two DPA groups play an important role in the detection of histidine.
Detailed Optimization of Polycondensation Reaction via Direct C–H Arylation of Ethylenedioxythiophene
Authors: Yamazaki Koutarou , Kuwabara Junpei , Kanbara Takaki 
Published: October 2012

AbstractThe polycondensation reaction of 3,4‐ethylenedioxythiophene with 2,7‐dibromo‐9,9‐dioctylfluorene via Pd‐catalyzed direct arylation gives poly[(3,4‐ethylenedioxythiophene‐2,5‐diyl)‐(9,9‐dioctylfluorene‐2,7‐diyl)]. The reaction conditions are optimized in terms of the Pd precatalysts, reaction time, and carboxylic acid additives. The combination of 1 mol% Pd(OAc)2 and 1‐adamantanecarboxylic acid as an additive is the optimized catalytic system, and it yields the corresponding polymer with a molecular weight of 39 400 in 89% yield. The polycondensation reaction, followed by an end‐capping reaction, effectively provides a linear polymer without Br terminals.
Pure Protein Scaffolds from Pickering High Internal Phase Emulsion Template
Authors: Li Zifu , Xiao Manda , Wang Jianfang , Ngai To 
Published: October 2012

AbstractThe formation of hierarchical porous protein scaffolds from oil‐in‐water (o/w) high internal phase emulsions (HIPEs) stabilized by bovine serum albumin (BSA) protein nanoparticles (Pickering HIPE) is reported. The route consists of three principal steps. First, a stable o/w HIPE stabilized by BSA protein nanoparticles is formulated. Next, crosslinking the dispersed protein nanoparticles gives rise to a gel in the continuous water phase to freeze the emulsion's microstructure. Finally, removal of the oil components and water directly leads to a three dimensional, bimodal meso‐macroporous protein scaffold, which is suitable for a wide range of biomedical applications.
Polyester production by halophilic and halotolerant bacterial strains obtained from mangrove soil samples located in Northern Vietnam
Authors: Van‐Thuoc Doan , Huu‐Phong Tran , Thi‐Binh Nguyen , Thi‐Tho Nguyen , Minh‐Lam Duong , Quillaguamán Jorge 
Published: October 2012

AbstractThis research article reports halophilic and halotolerant bacteria isolated from mangrove forests located in Northern Vietnam. Several of these bacteria were able to synthesize polyhydroxyalkanoates (PHAs). PHAs are polyesters stored by microorganisms under the presence of considerable amounts of a carbon source and deficiency of other essential nutrient such as nitrogen or phosphorous. Mangrove forests in Northern Vietnam are saline coastal habitats that have not been microbiologically studied. Mangrove ecosystems are, in general, rich in organic matter, but deficient in nutrients such as nitrogen and phosphorus. We have found about 100 microorganisms that have adapted to mangrove forests by accumulating PHAs. The production of polyesters might therefore be an integral part of the carbon cycle in mangrove forests. Three of the strains (ND153, ND97, and QN194) isolated from the Vietnamese forests were identified as Bacillus species, while other five strains (QN187, ND199, ND218, ND240, and QN271) were phylogenetically close related to the α‐proteobacterium Yangia pacifica. These strains were found to accumulate PHAs in noticeable amounts. Polymer inclusions and chemical structure were studied by transmission electron microscopy and proton nuclear magnetic resonance (NMR) spectroscopy analyses, respectively. Strains ND153, ND97, QN194, QN187, ND240, and QN271 synthesized poly(3‐hydroxybutyrate) (PHB) from glucose, whereas strains ND199 and ND218 synthesized poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) (PHBV) from this carbohydrate. With the exception of strain QN194, the strains accumulated PHBV when a combination of glucose and propionate was included in the culture medium. The polymer yields and cell growth reached by one Bacillus isolate, strain ND153, and one Gram‐negative bacterium, strain QN271, were high and worth to be researched further. For experiments performed in shake flasks, strain ND153 reached a maximum PHBV yield of 71 wt% and a cell dry weight (CDW) of 3.6 g/L while strain QN271 attained a maximum PHB yield of 48 wt% and a CDW of 5.1 g/L. Both strain ND153 and strain QN271 may only represent a case in point that exemplifies of the potential that mangrove forests possess for the discovery of novel halophilic and halotolerant microorganisms able to synthesize different types of biopolyesters.
The differential susceptibility of spores from virulent and attenuated Bacillus anthracis strains to aldehyde‐ and hypochlorite‐based disinfectants
Authors: March Jordon K. , Cohen Marissa N. , Lindsey James M. , Millar D. A. , Lowe Chinn‐Woan , Bunnell Annette J. , O'Neill Kim L. , Bruce Schaalje G. , Robison Richard A. 
Published: October 2012

AbstractThis study compared the sensitivity of spores from virulent and attenuated Bacillus anthracis strains in suspension to inactivation by various chemical disinfectants. Spore suspensions from two virulent strains (A0256 and A0372) and two attenuated strains (Sterne and A0141) of B. anthracis were tested against two aldehyde‐based disinfectants and one hypochlorite‐based disinfectant. A novel statistical model was used to estimate 4‐log10 reduction times for each disinfectant/strain combination. Reduction times were compared statistically using approximate Z and χ2 tests. Although there was no consistent correlation between virulence and increased sporicidal resistance across all three disinfectants, spores from the two virulent and two attenuated strains did display significantly different susceptibilities to different disinfectants. Significant disinfectant–strain interactions were observed for two of the three disinfectants evaluated. The comparative results suggest that the use of surrogate organisms to model the inactivation kinetics of virulent B. anthracis spores may be misleading. The accuracy of such extrapolations is disinfectant dependent and must be used with caution.
Numerical analysis of thermal fracturing in subsurface cold water injection by finite element methods
Authors: Yin Shunde 
Published: October 2012

SUMMARYIntegration of poromechanics and fracture mechanics plays an important role in understanding a series of thermal fracturing phenomena in subsurface porous media such as cold water flooding for enhanced oil recovery, produced‐water reinjection for waste disposal, cold water injection for geothermal energy extraction, and CO2injection for geosequestration. Thermal fracturing modeling is important to prevent the potential risks when fractures propagate into undesired zones, and it involves the coupling of heat transfer, mass transport, and stress change as well as the fracture propagation. Analytical method, finite element method, and finite difference method as well as boundary element method have been used to perform the thermal fracturing modeling considering different degrees and combinations of coupling. In this paper, extended finite element method is employed for the thermal fracturing modeling in a fully coupled fashion with remeshing avoided, and the stabilized finite element method is employed to account for the convection‐dominated heat transfer in the fracturing process with numerical oscillation circumvented. With the thermal fracturing model, a hypothetical numerical experiment on cold water injection into a deep warm aquifer is conducted. Results show that parameters such as injection rate, injection temperature, aquifer stiffness, and permeability can affect the fracture development in different ways and extended finite element method and stabilized finite element method provide effective tools for thermal fracturing simulation. Copyright © 2012 John Wiley & Sons, Ltd.
A multidisciplinary approach can help both Parkinson's and epilepsy patients
Authors: Church Alistair , Lanyon Karen 
Published: October 2012

AbstractWe are pleased to introduce a new series of Commentaries from the Primary Care Neurology Society (P‐CNS). These aim to provide a primary care perspective on the neurology articles featured in each issue of Progress. Here, Dr Alistair Church provides a commentary on our Review ‘Diagnosis and management of psychosis in Parkinson's disease’ (see page 7 of this issue) and Dr Karen Lanyon gives her perspective on ‘Improving quality of care for epilepsy patients using a pharmacist review service’ (page 12).
Improving quality of care for epilepsy patients using a pharmacist review service
Authors: Brown Carole 
Published: October 2012

AbstractA structured review process for epilepsy in primary care has been advocated as best practice. In Calderdale Primary Care Trust, a prescribing clinical pharmacist practitioner with a special interest in epilepsy (PwSI epilepsy) has been conducting annual reviews for epilepsy patients in GP practices. This service has improved patient care over and above the Quality and Outcomes Framework (QOF) indicators for epilepsy and has been shown to be sustainable.
Synthesis and thin‐film orientation of poly(styrene‐block‐trimethylsilylisoprene)
Authors: Bates Christopher M. , Pantoja Marco A. Bedolla , Strahan Jeffrey R. , Dean Leon M. , Mueller Brennen K. , Ellison Christopher J. , Nealey Paul F. , Willson C. Grant 
Published: October 2012

AbstractThe successful synthesis, characterization, and directed self‐assembly of a silicon‐containing block copolymer, poly(styrene‐block‐trimethylsilylisoprene) (P(S‐b‐TMSI)), which has much higher oxygen etch contrast than the de facto standard, poly(styrene‐block‐methyl methacrylate) is reported. A Sakurai, Grignard‐type coupling reaction provided the key monomer in good yield. Living anionic polymerization was employed to prepare the block copolymer, which has very low polydispersity. P(S‐b‐TMSI) was successfully ordered and oriented by directed self‐assembly. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Synthesis, characterization, and catalytic activity of titanium iminophenoxide complexes in relation to the ring‐opening polymerization of L‐lactide and ε‐caprolactone
Authors: Chen Hsuan‐Ying , Lu Wei‐Yi , Chen Yen‐Jen , Hsu Sodio C. N. , Ou Siou‐Wei , Peng Wei‐Te , Jheng Nai‐Yuan , Lai Yi‐Chun , Wu Bo‐Sheng , Chung Hsuan , Chen Yun , Huang Ta‐Chou 
Published: October 2012

AbstractThis study synthesized a series of titanium iminophenoxide complexes and investigated their suitability as catalysts for the ring‐opening polymerization of L‐lactide (L‐LA) and ε‐caprolactone (CL). Complexes with bidentate ligands demonstrate higher catalytic activity than their tridentate counterparts since the third coordination atom needs to contend with L‐LA and CL. Differences in the geometric framework of bidentate ligands also influence the catalytic activity. Type II ligands (N, N‐trans form of Ti complex) prevent the coordination of monomers to Ti thereby decreasing the initiation rate. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2013
Fracture behavior of amorphous and semicrystalline blends of poly(vinylidene fluoride) and poly(methyl methacrylate)
Authors: Laiarinandrasana Lucien , Nziakou Yannick , Halary Jean Louis 
Published: October 2012

AbstractThe fracture behavior of blends of poly(vinylidene fluoride) and poly(methyl methacrylate) was investigated all over the composition range. A detailed analysis of the net stress versus crack opening displacement curves was performed. Fracture surface observations allowed statements on the process zone characteristics ahead of the crack tip. For the amorphous blends, the crack initiation energy is well related to the glass transition temperature. For the semicrystalline blends, the fracture energy is correlated with the degree of crystallinity. © 2012 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012