bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of shear creep properties of wood via modified Burger models and off-axis compression test method
Authors: Shimazaki Kanon , Ando Kosei 
Published: July 2024

AbstractIn this study, the rheological Burger model combining Maxwell and Voigt–Kelvin model units as well as modified mechanical models were employed to analyze the shear creep mechanism of wood. Off-axis compression tests were conducted on Japanese Hinoki cypress specimens (Chamaecyparis obtusa), and a mechanical analysis of the shear creep mechanism was performed. First, the measured creep compliance curves [JTL(t)] were fitted using this Burger model, which is a typical model used to explain the creep behavior of wood. Furthermore, three modified Burger models with non-Newtonian dashpots were proposed to explain the measured data more accurately: model 1—only the dashpot in the permanent strain unit is non-Newtonian; model 2—both dashpots are non-Newtonian; and model 3—only the dashpot in the delayed elastic strain unit is non-Newtonian. The mean value of the coefficient of determination was highest for model 1. The number of specimens that could be fitted with a tolerance error of 0.1% was 43 out of 50 with the Burger model, 45 with model 1, 25 with model 2, and 45 with model 3. The Burger model exhibited large discrepancies between the theoretical and measured values, model 2 could not be used to explain several specimens, and model 3 exhibited a delayed elastic strain behavior that was inconsistent with the definition. Therefore, we conclude that model 1 is the most appropriate for studying the shear creep behavior of wood.
Lattices of varieties of plactic-like monoids
Authors: Aird Thomas , Ribeiro Duarte 
Published: July 2024

AbstractWe study the equational theories and bases of meets and joins of several varieties of plactic-like monoids. Using those results, we construct sublattices of the lattice of varieties of monoids, generated by said varieties. We calculate the axiomatic ranks of their elements, obtain plactic-like congruences whose corresponding factor monoids generate varieties in the lattice, and determine which varieties are joins of the variety of commutative monoids and a finitely generated variety. We also show that the hyposylvester and metasylvester monoids generate the same variety as the sylvester monoid.
Carbon and Nutrient Limitations of Microbial Metabolism in Xingkai Lake, China: Abiotic and Biotic Drivers
Authors: Chen Xingting , Zhang Weizhen , Geng Mengdie , Shen Ji , Wang Jianjun 
Published: July 2024

AbstractMicrobial communities are crucial for water quality and biogeochemical cycling in freshwaters. Microbes secrete extracellular enzymes to decompose organic matter for their needs of nutrients and scarce elements. Yet, there is a lack of knowledge on microbial metabolic limitations in freshwaters, especially in lake sediments. Here, we examined the carbon, nitrogen, and phosphorus-acquiring extracellular enzyme activities and the bacterial and fungal communities of 30 sediments across Xingkai Lake, the largest freshwater lake in Northeast Asia. We further analyzed the microbial metabolic limitations via extracellular enzyme stoichiometry and explored the direct and indirect effects of abiotic and biotic factors on the limitations. We found that microbial metabolisms were primarily limited by phosphorus in Xingkai Lake. For instance, microbial carbon and phosphorus limitations were closely correlated to abiotic factors like water depth, total dissolved solids, sediment total carbon, and conductivity. The metabolic limitations were also affected by biotic factors, such as showing positive relationships with the alpha and beta diversity of bacteria, and with the beta diversity of fungi. In addition, community compositions of bacteria and fungi were mainly correlated to abiotic factors such as total carbon and dissolved organic carbon, respectively. Collectively, microbial metabolic limitations were affected directly or indirectly by abiotic factors and microbial communities. Our findings indicate that microbial metabolic limitations are not only driven by bacteria and fungi but also by abiotic factors such as water depth and total nitrogen, and thus provide empirical evidence for effective management of freshwater lakes under climate warming and intensified human activities.
Fungal aerosol and particulate matter in horse stables in Poland
Authors: Grzyb Jacek , Podstawski Zenon , Bulski Karol 
Published: July 2024

Abstract
Horses stay in different types of stables; especially during the cold season, they stay inside for most of the day. A stable is also a place where many people spend quite a lot of time either as employees who care for and train horses or as equine enthusiasts. Keeping horses in stables causes their constant exposure to high concentrations of particulate matter (PM) and molds in the air inside these facilities. The study was conducted in Udórz Stud Farm located in the southern region of Poland. It was carried out in two different types of stables: three runners and two box stables. The study continued for 2 years; samples were collected in each season of the year. The following devices were used: a six-stage Andersen-Graseby cascade impactor, the DustTrak™ II Aerosol Monitor 8530. The obtained results allowed for the conclusion that horses kept in box stables are exposed to lower concentrations of molds and yeasts than those kept in runners. Molds dominated in the stable air during humid periods—spring and autumn—while yeasts were more prominent during summer and winter. It was observed that cleaning stables reduces the morphotic elements of fungi in the air, even though it results in a higher level of particulate matter in the stable air. It should be noted that microclimate conditions were optimal for horses practically throughout the whole year.

Key points
• In stables, there is a high level of air intoxication, both by yeast and by mold fungi
• The concentrations of fungi in the air depend on the season and the stable cleaning procedure
• The PM concentrations depend on the type of stable
Exploring TNFi drug-levels and anti-drug antibodies during tapering among patients with inflammatory arthritis: secondary analyses from the randomised BIODOPT trial
Authors: Uhrenholt Line , Sørensen Mads E. R. , Lauridsen Karen B. , Duch Kirsten , Dreyer Lene , Christensen Robin , Hauge Ellen-Margrethe , Loft Anne Gitte , Rasch Mads N. B. , Horn Hans Christian , Taylor Peter C. , Nielsen Kaspar R. , Kristensen Salome 
Published: July 2024

AbstractTo evaluate tumour necrosis factor inhibitor (TNFi) drug-levels and presence of anti-drug antibodies (ADAb) in patients with inflammatory arthritis who taper TNFi compared to TNFi continuation. Patients with rheumatoid arthritis, psoriatic arthritis, or axial spondyloarthritis on stable TNFi dose and in low disease activity ≥ 12 months were randomised (2:1) to disease activity-guided tapering or control. Blood samples at baseline, 12- and 18-months were evaluated for TNFi drug-levels and ADAb. In total, 129 patients were randomised to tapering (n = 88) or control (n = 41). Between baseline and month 18, a significant shift in TNFi drug-levels were observed in the tapering group resulting in fewer patients with high drug-levels (change: − 14% [95% CI − 27 to − 1%]) and more with low drug-levels (change: 18% [95% CI 5–31%]). Disease activity was equivalent between groups at 18 months, mean difference: RA − 0.06 (95% CI − 0.44 to 0.33), PsA 0.03 (95% CI − 0.36 to 0.42), and axSpA 0.16 (− 0.17 to 0.49), equivalence margins ± 0.5 disease activity points. ADAb were detected in eight patients, all from the tapering group. TNFi drug-level category or ADAb were not predictive for achieving successful tapering at 18 months. TNFi drug-levels decreased during tapering which indicate adherence to the tapering algorithm. Despite the difference in TNFi drug-levels at 18 months, disease activity remained equivalent, and only few tapering patients had detectable ADAb. These data do not support using TNFi drug-level and/or ADAb to guide the tapering decision but future research with larger trials is needed.Trial registration: EudraCT: 2017-001970-41, December 21, 2017.
RNA interference-based strategies to control Botrytis cinerea infection in cultivated strawberry
Authors: Capriotti Luca , Molesini Barbara , Pandolfini Tiziana , Jin Hailing , Baraldi Elena , Cecchin Michela , Mezzetti Bruno , Sabbadini Silvia 
Published: July 2024

Abstract
Key message
Gene silencing of BcDCL genes improves gray mold disease control in the cultivated strawberry.

Abstract
Gene silencing technology offers new opportunities to develop new formulations or new pathogen-resistant plants for reducing impacts of agricultural systems. Recent studies offered the proof of concept that the symptoms of gray mold can be reduced by downregulating Dicer-like 1 (DCL1) and 2 (DCL2) genes of Botrytis cinerea. In this study, we demonstrate that both solutions based on dsRNA topical treatment and in planta expression targeting BcDCL1 and BcDCL2 genes can be used to control the strawberry gray mold, the most harmful disease for different fruit crops. 50, 70 and 100 ng μL−1 of naked BcDCL1/2 dsRNA, sprayed on plants of Fragaria x ananassa cultivar Romina in the greenhouse, displayed significant reduction of susceptibility, compared to the negative controls, but to a lesser extent than the chemical fungicide. Three independent lines of Romina cultivar were confirmed for their stable expression of the hairpin gene construct that targets the Bc-DCL1 and 2 sequences (hp-Bc-DCL1/2), and for the production of hp construct-derived siRNAs, by qRT-PCR and Northern blot analyses. In vitro and in vivo detached leaves, and fruits from the hp-Bc-DCL1/2 lines showed significantly enhanced tolerance to this fungal pathogen compared to the control. This decreased susceptibility was correlated to the reduced fungal biomass and the downregulation of the Bc-DCL1 and 2 genes in B. cinerea. These results confirm the potential of both RNAi-based products and plants for protecting the cultivated strawberry from B. cinerea infection, reducing the impact of chemical pesticides on the environment and the health of consumers.
The long-term learning curve of holmium laser enucleation of the prostate (HoLEP) in the en-bloc technique: a single surgeon series of 500 consecutive cases
Authors: Wenk M. J. , Hartung F. O. , Egen L. , Netsch C. , Kosiba M. , Grüne B. , Herrmann Jonas 
Published: July 2024

Abstract

Purpose
To evaluate perioperative parameters, clinical outcomes, and the learning curve of holmium laser enucleation of the prostate (HoLEP) of a single surgeon in 500 consecutive cases.

Methods
Demographic parameters, outcomes, and adverse events were evaluated. The learning curve for HoLEP in en-bloc technique of the first 500 consecutive patients was analyzed in clusters of 100 (clusters 1–5) using the Wilcoxen rank test, Chi² test and Kruskal Wallis test.

Results
Enucleation weight was similar in the clusters 1,2,3, and 5 (62 g, 63 g, 61 g, 61 g), in cluster 4 it was slightly higher at 73 g. There was a significant reduction in operating time from 67 min (cluster 1) to 57 min (cluster 2), 46 min (cluster 3), 53 min (cluster 4), and 43 min (cluster 5), p < 0.001. Enucleation efficiency (g/min) showed a steady increase (1.72, 2.24, 2.79, 2.92 vs. 2.99, p < 0.001). Laser energy efficiency also improved (2.17 vs. 2.12 vs. 1.71 vs. 1.65 vs. 1.55; p < 0.001). There was no measurable learning curve regarding the length of hospital stay (mean 2.5 days), catheterization time (1.9 days), hemoglobin drop (approx. 1 g/dl) or complications (p > 0.1).

Conclusions
HoLEP using the en-bloc technique is a safe and highly effective method. Over time, a slight but steady learning curve and improvement in operation time, enucleation efficiency and laser energy efficiency were shown even for an experienced surgeon - after 500 cases, still no plateau was reached. There was no measurable learning curve regarding blood loss, complications, length of hospital stay, and catheterization time.
The role of VI-RADS scoring criteria for predicting oncological outcomes in bladder cancer
Authors: Kozikowski Mieszko , Zagrodzka Magdalena , Zawadzki Marek , Zugaj Przemysław , Osiecki Rafał , Rzymkowski Franciszek , Śledź Mateusz , Sosnowski Roman , Dobruch Jakub , Giudice Francesco Del , Krajewski Wojciech 
Published: July 2024

Abstract
Purpose
Our purpose was to evaluate the prognostic value of Vesical Imaging Reporting and Data System (VI-RADS) in bladder cancer (BCa) staging and predicting recurrence or progression.

Methods
We retrospectively analyzed the prospectively collected data from 96 patients with bladder tumors who underwent VI-RADS-based multiparametric magnetic resonance imaging (mpMRI) before endourological treatment from April 2021 to December 2022. Diagnostic performance was evaluated by comparing mpMRI reports with final pathology, using logistic regression for muscle-invasive bladder cancer (MIBC) predictors. Follow-up until May 2023 included Kaplan-Meier and Cox regression analysis to assess VI-RADS predictive roles for recurrence-free survival (RFS) and progression-free survival (PFS).

Results
A total of 96 patients (19.8% women, 80.2% men; median age 68.0 years) were included, with 71% having primary tumors and 29% recurrent BCa. Multiparametric MRI exhibited high sensitivity (92%) and specificity (79%) in predicting MIBC, showing no significant differences between primary and recurrent cancers (AUC: 0.96 vs. 0.92, P = .565). VI-RADS emerged as a key predictor for MIBC in both univariate (OR: 40.3, P < .001) and multivariate (OR: 54.6, P < .001) analyses. Primary tumors with VI-RADS ≥ 3 demonstrated significantly shorter RFS (P = .02) and PFS (P = .04).

Conclusions
In conclusion, mpMRI with VI-RADS has a high diagnostic value in predicting MIBC in both primary and recurrent BCa. A VI-RADS threshold ≥ 3 is a strong predictor for MIBC, and in primary tumors predicts early recurrence and progression.
Visualization of the two-phase flow in the air gap of an optically accessible generic electric motor and its effect on torque
Authors: Auernhammer A. , Schary S. , Dreizler A. , Böhm B. 
Published: July 2024

AbstractElectric motors with high-power densities are required for the implementation of electromobility. To achieve this, direct liquid cooling methods are increasingly being considered, in which oil is injected into the motor compartment. This results in a two-phase flow that can be used for efficient cooling. However, the oil, which can also penetrate the air gap between the rotor and stator, can also lead to additional losses due to increased friction. Since little is known about the two-phase flow in such systems, especially in the air gap, it is investigated by means of simple optical visualizations and high-speed laser-induced fluorescence imaging as well as torque measurements. The measurements are carried out in the air gap of an optically accessible generic model of a directly cooled electric motor. Speed variations were performed from 100 to 2000 rpm, and three different two-phase flow regimes were observed. At low speeds (Flow Regime 1), the air gap is filled locally with oil in radial direction, in the medium speed range (Flow Regime 2) with foam, while at high speeds (Flow Regime 3) separated films were observed on the rotor and stator. The torque difference between the two-phase and single-phase operation, which quantifies the mechanical losses due to the injected oil, increased continuously due to the oil in the air gap until it reached a maximum in Flow Regime 2 due to foam formation. In Flow Regime 3, the torque difference was negative. This was attributed to the fact that the grooves in the stator were filled with oil, thus reducing the turbulence generation of the air flow.
Role of blood metabolites in mediating the effect of gut microbiome on the mutated-RAS/BRAF metastatic colorectal cancer-specific survival
Authors: Xiang Yaoxian , Zhang Chan , Wang Jing , Cheng Yurong , Wang Kangjie , Wang Li , Tong Yingying , Yan Dong 
Published: July 2024

Abstract
Background
Recent studies have linked alterations in the gut microbiome and metabolic disruptions to the invasive behavior and metastasis of colorectal cancer (CRC), thus affecting patient prognosis. However, the specific relationship among gut microbiome, metabolite profiles, and mutated-RAS/BRAF metastatic colorectal cancer (M-mCRC) remains unclear. Furthermore, the potential mechanisms and prognostic implications of metabolic changes induced by gut microbiome alterations in patients with M-mCRC still need to be better understood.

Methods
We conducted Mendelian randomization (MR) to evaluate the causal relationship of genetically predicted 196 gut microbiome features and 1400 plasma metabolites/metabolite ratios on M-mCRC-specific survival. Additionally, we identified significant gut microbiome-metabolites/metabolite ratio associations based on M-mCRC. Metabolite information was annotated, and functional annotation and pathway enrichment analyses were performed on shared proteins corresponding to significant metabolite ratios, aiming to reveal potential mechanisms by which gut microbiome influences M-mCRC prognosis via modulation of human metabolism.

Results
We identified 11 gut microbiome features and 49 known metabolites/metabolite ratios correlated with M-mCRC-specific survival. Furthermore, we identified 17 gut microbiome-metabolite/metabolite ratio associations specific to M-mCRC, involving eight lipid metabolites and three bilirubin degradation products. The shared proteins corresponding to significant metabolite ratios were predominantly localized within the integral component of the membrane and exhibited enzymatic activities such as glucuronosyltransferase and UDP-glucuronosyltransferase, crucial in processes such as glucuronidation, bile secretion, and lipid metabolism. Moreover, these proteins were significantly enriched in pathways related to ascorbate and aldarate metabolism, pentose and glucuronate interconversions, steroid hormone biosynthesis, and bile secretion.

Conclusion
Our study offers novel insights into the potential mechanisms underlying the impact of the gut microbiome on the prognosis of M-mCRC. These findings serve as a meaningful reference for exploring potential therapeutic targets and strategies in the future.
Empagliflozin prevents heart failure through inhibition of the NHE1-NO pathway, independent of SGLT2
Authors: Chen Sha , Wang Qian , Bakker Diane , Hu Xin , Zhang Liping , van der Made Ingeborg , Tebbens Anna M. , Kovácsházi Csenger , Giricz Zoltán , Brenner Gábor B. , Ferdinandy Peter , Schaart Gert , Gemmink Anne , Hesselink Matthijs K. C. , Rivaud Mathilde R. , Pieper Michael P. , Hollmann Markus W. , Weber Nina C. , Balligand Jean-Luc , Creemers Esther E. , Coronel Ruben , Zuurbier Coert J. 
Published: July 2024

AbstractSodium glucose cotransporter 2 inhibitors (SGLT2i) constitute the only medication class that consistently prevents or attenuates human heart failure (HF) independent of ejection fraction. We have suggested earlier that the protective mechanisms of the SGLT2i Empagliflozin (EMPA) are mediated through reductions in the sodium hydrogen exchanger 1 (NHE1)-nitric oxide (NO) pathway, independent of SGLT2. Here, we examined the role of SGLT2, NHE1 and NO in a murine TAC/DOCA model of HF. SGLT2 knockout mice only showed attenuated systolic dysfunction without having an effect on other signs of HF. EMPA protected against systolic and diastolic dysfunction, hypertrophy, fibrosis, increased Nppa/Nppb mRNA expression and lung/liver edema. In addition, EMPA prevented increases in oxidative stress, sodium calcium exchanger expression and calcium/calmodulin-dependent protein kinase II activation to an equal degree in WT and SGLT2 KO animals. In particular, while NHE1 activity was increased in isolated cardiomyocytes from untreated HF, EMPA treatment prevented this. Since SGLT2 is not required for the protective effects of EMPA, the pathway between NHE1 and NO was further explored in SGLT2 KO animals. In vivo treatment with the specific NHE1-inhibitor Cariporide mimicked the protection by EMPA, without additional protection by EMPA. On the other hand, in vivo inhibition of NOS with L-NAME deteriorated HF and prevented protection by EMPA. In conclusion, the data support that the beneficial effects of EMPA are mediated through the NHE1-NO pathway in TAC/DOCA-induced heart failure and not through SGLT2 inhibition.
Voice quality after open partial horizontal laryngectomy vs. total laryngectomy with voice prosthesis: a comparative study
Authors: Botti Cecilia , Lotto Cecilia , Tesauro Paolo , Guidotti Monica , Borghi Aurora , Molteni Gabriele , Presutti Livio , Fernandez Ignacio Javier 
Published: July 2024

Abstract
Objectives
The aim of this study is to compare voice outcomes in open partial horizontal laryngectomy vs. total laryngectomy (TL) with voice prosthesis.

Methods
In this retrospective monocentric study patients undergoing OPHL or TL with voice prosthesis were enrolled during the usual oncological follow-up consultations at the Otolaryngology and Audiology Unit of a University Hospital in the period between July 2022 and June 2023. Acoustic analysis (F0, HNR, NHR), maximum phonation time, I-SECEL and INFV0 scale were used to assess voice outcome.

Results
Forty-three patients were enrolled. Voices of patients undergoing LT were better in quality of voice (V0) at INFV0 scale. The scores in I-SECEL and acoustic analysis were comparable.

Conclusions
Voice quality could be slight better in patients undergoing TL with voice prosthesis than those undergoing OPHL.