bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fetal serum α‐1 microglobulin for renal function assessment: comparison with β2‐microglobulin and cystatin C
Authors: Nguyen Claire , Dreux Sophie , Heidet Laurence , Czerkiewicz Isabelle , Salomon Laurent J. , Guimiot Fabien , Schmitz Thomas , Tsatsaris Vassilis , Boulot Pierre , Rousseau Thierry , Muller Françoise 
Published: April 2013

ABSTRACTObjectiveTo compare the prognostic value of fetal serum α1‐microglobulin with that of β2‐microglobulin and cystatin C for postnatal renal function.MethodRetrospective study of α1‐microglobulin, β2‐microglobulin, and cystatin C in fetal serum from 126 fetuses with congenital abnormalities of the kidney and urinary tract (73 and 53, respectively). Two groups were defined: group with normal renal function and group with renal failure. For live born infants, renal function was assessed on the basis of serum creatinine (cutoff 50 µmol/L) or glomerular filtration rate (cutoff 75 mL/min/1.73 m2) or both. In case of infant or fetal death, histological kidney lesions were considered.ResultsSignificant differences (p < 0.001) were observed for the three markers between fetuses with good renal prognosis and those with renal failure (34.4 mg/L vs 67.6 mg/L for α1‐microglobulin, respectively; 3.9 mg/L vs 7.35 mg/L, for β2‐microglobulin, respectively; and 1.67 mg/L vs 2.12 mg/L for cystatin C, respectively). Areas under receiver operator curves were used to compare the three markers, 0.96, 0.90, and 0.74 for β2‐microglobulin, α1‐microglobulin, and cystatin C, respectively.ConclusionAlthough α1‐microglobulin is significantly different in fetuses with good renal prognosis and those with renal failure, overall, it is a less reliable prognostic marker than fetal serum β2‐microglobulin. © 2013 John Wiley & Sons, Ltd.
Maternal serum cytokines at 30–33 weeks in the prediction of preeclampsia
Authors: Mosimann Beatrice , Wagner Marion , Poon Leona C.Y. , Bansal Amolak S. , Nicolaides Kypros H. 
Published: April 2013

AbstractObjectiveThe aim of this case–control study at 30–33 weeks, a few days or weeks before the clinical onset of preeclampsia (PE), was to assess whether serum concentrations of cytokines differ between patients who are destined to develop PE and those with uncomplicated pregnancies.MethodsA panel of cytokines was measured using Luminex technology at 30–33 weeks' gestation in 39 cases that developed PE at or after 34 weeks and 117 unaffected controls.ResultsThe serum concentrations of most analysed cytokines were no different in women who developed PE than in controls. The proportions of women with detectable concentrations of MIP‐1α and IL‐8 were significantly lower in those with PE than in the controls (MIP‐1α: 14/39 vs 76/117, P = 0.003; IL‐8:13/39 vs 83/117, P < 0.0001). The median maternal serum concentration of IL‐1β was significantly lower in the PE cases than in the controls (0.38 pg/mL, range 0.01–0.92, vs 0.60 pg/mL, range 0.02–3.54, P = 0.005).ConclusionOur findings do not lend support to the hypothesis that systemic inflammation precedes the onset of PE or that cytokines are good markers for such inflammation and certainly the panel of cytokines we examined does not provide useful prediction of subsequent development of PE. © 2013 John Wiley & Sons, Ltd.
Synthesis and characterization of novel adamantane‐based copoly(aryl ether ketone)s with low dielectric constants
Authors: Geng Zhi , Lu Yaning , Zhang Shuling , Jiang Xu , Huo Pengfei , Luan Jiashuang , Wang Guibin 
Published: April 2013

AbstractA series of novel adamantane‐based copoly(aryl ether ketone)s (PAEK‐CF3‐Ad) with low dielectric constants were prepared by post‐amidation of copoly(aryl ether ketone)s containing (3‐trifluoromethyl)phenyl and carboxyl groups with 4‐adamantylaniline. Compared with the preparation of adamantane‐containing poly(aryl ether ketone)s by direct polymerization of adamantyl‐substituted monomers, this side‐chain grafting method avoids various problems, such as high polymerization temperature and the difficult polymerization of adamantyl‐substituted monomers. The dielectric, thermal and mechanical properties of the synthesized PAEK‐CF3‐Ad were characterized using a precision impedance analyzer, differential scanning calorimetry and thermogravimetric analysis, and a universal tester, respectively. The results indicate that PAEK‐CF3‐Ad films exhibited low dielectric constants ranging from 2.33 to 2.65 at 1 MHz due to the introduction of the adamantyl groups. The synthesized PAEK‐CF3‐Ad copolymers exhibited good thermal and mechanical properties. © 2013 Society of Chemical Industry
A biofeedback intervention to control impulsiveness in a severely personality disordered forensic patient
Authors: Howard Rick , Schellhorn Klaus , Lumsden John 
Published: April 2013

ABSTRACTImpulsiveness in personality disordered forensic patients is associated with poor treatment completion and high risk of re‐offending. A biofeedback training protocol, previously found to reduce impulsiveness and improve attention in children with Attention Deficit Hyperactivity Disorder, was used in an initial attempt to reduce impulsiveness in a severely personality disordered man with borderline, antisocial and histrionic features. Electrocortical, behavioural and self‐report measures of impulsiveness were taken before and immediately following 6 weeks of biofeedback training and at 3 months follow‐up. The patient successfully engaged with the intervention. His self‐reports of reduced impulsiveness and improved attention were corroborated by behavioural and electrocortical measures that indicated reduced impulsiveness and better focused attention. Results suggest this intervention might prove useful in improving behavioural and emotional self‐regulation in severely personality disordered patients. Copyright © 2013 John Wiley & Sons, Ltd.
Proteomic analyses of serous and endometrioid epithelial ovarian cancers – Cases studies – Molecular insights of a possible histological etiology of serous ovarian cancer
Authors: Longuespée Rémi , Gagnon Hugo , Boyon Charlotte , Strupat Kurstin , Dauly Claire , Kerdraon Olivier , Ighodaro Adesuwa , Desmons Annie , Dupuis Jocelyn , Wisztorski Maxence , Vinatier Denis , Fournier Isabelle , Day Robert , Salzet Michel 
Published: April 2013

PurposeEpithelial ovarian carcinogenesis may occur de novo on the surface of ovarian mesothelial epithelial cells or from cells originating in other organs. Foreign Müllerian cell intrusion into the ovarian environment has been hypothesized to explain the latter scenario. In this study, MALDI MS profiling technology was used to provide molecular insights regarding these potentially different mechanisms.Experimental designUsing MALDI MS profiling, the molecular disease signatures were established in their anatomical context. MALDI MS profiling was used on serous and endometrioid cancer biopsies to investigate cases of epithelial ovarian cancer. We then applied bioinformatic methods and identification strategies on the LC‐MS/MS analyses of extracts from digested formalin‐fixed, paraffin‐embedded tissues. Extracts from selected regions (i.e. serous ovarian adenocarcinoma, fallopian tube serous adenocarcinoma, endometrioid ovarian cancer, benign endometrium, and benign ovarian tissues) were performed, and peptide digests were subjected to LC‐MS/MS analysis.ResultsComparison of the proteins identified from benign endometrium or three ovarian cancer types (i.e. serous ovarian adenocarcinoma, endometrioid ovarian adenocarcinoma, and serous fallopian tube adenocarcinoma) provided new evidence of a possible correlation between the fallopian tubes and serous ovarian adenocarcinoma. Here, we propose a workflow consisting of the comparison of multiple tissues in their anatomical context in an individual patient.Conclusion and clinical relevanceThe present study provides new insights into the molecular similarities between these two tissues and an assessment of highly specific markers for an individualized patient diagnosis and care.
Trauma‐associated human neutrophil alterations revealed by comparative proteomics profiling
Authors: Zhou Jian‐Ying , Krovvidi Ravi K. , Gao Yuqian , Gao Hong , Petritis Brianne O. , De Asit K. , Miller‐Graziano Carol L. , Bankey Paul E. , Petyuk Vladislav A. , Nicora Carrie D. , Clauss Therese R. , Moore Ronald J. , Shi Tujin , Brown Joseph N. , Kaushal Amit , Xiao Wenzhong , Davis Ronald W. , Maier Ronald V. , Tompkins Ronald G. , Qian Wei‐Jun , Camp David G. , Smith Richard D. ,   the Inflammation and the Host Response to Injury Large Scale Collaborative Research Program
Published: April 2013

PurposePolymorphonuclear neutrophils (PMNs) play an important role in mediating the innate immune response after severe traumatic injury; however, the cellular proteome response to traumatic condition is still largely unknown.Experimental designWe applied 2D‐LC‐MS/MS‐based shotgun proteomics to perform comparative proteome profiling of human PMNs from severe trauma patients and healthy controls.ResultsA total of 197 out of ∼2500 proteins (being identified with at least two peptides) were observed with significant abundance changes following the injury. The proteomics data were further compared with transcriptomics data for the same genes obtained from an independent patient cohort. The comparison showed that the protein abundance changes for the majority of proteins were consistent with the mRNA abundance changes in terms of directions of changes. Moreover, increased protein secretion was suggested as one of the mechanisms contributing to the observed discrepancy between protein and mRNA abundance changes. Functional analyses of the altered proteins showed that many of these proteins were involved in immune response, protein biosynthesis, protein transport, NRF2‐mediated oxidative stress response, the ubiquitin‐proteasome system, and apoptosis pathways.Conclusions and clinical relevanceOur data suggest increased neutrophil activation and inhibited neutrophil apoptosis in response to trauma. The study not only reveals an overall picture of functional neutrophil response to trauma at the proteome level, but also provides a rich proteomics data resource of trauma‐associated changes in the neutrophil that will be valuable for further studies of the functions of individual proteins in PMNs.
Individual and US County Determinants of Repeat Migration: a Comparison of Whites, Blacks, and Hispanics
Authors: Pais Jeremy 
Published: April 2013

ABSTRACTContemporary internal migration trends in the US raise questions about the role of community characteristics in shaping individual‐level migration propensities among different racial and ethnic groups. To examine this issue more closely, this research incorporates key county‐level characteristics into a study of repeat migration. With data from the 1979 National Longitudinal Survey of Youth merged with US census data, this study found that heavily populated counties and counties with large concentrations of employment in manufacturing industries reduce the odds of primary outmigration, onward migration to other locations, and return migration to a previous county of residence. Counties with a high degree of natural amenity retain longstanding white residents. Net of individual unemployment, the county‐level unemployment rate hinders primary and onward migration among whites. In support of the cultural constraints hypothesis – a hypothesis that anticipates divergent community‐level effects for minority and majority group members – whites are more likely to engage in repeat migration from counties with smaller shares of non‐Hispanic White population whereas blacks are more likely to engage in repeat migration from areas with larger shares of non‐Hispanic White population. whites and blacks are more likely to move out of counties with larger shares of foreign‐born population. The share of neither non‐Hispanic White population nor foreign‐born population affects Hispanic repeat migration propensities. Copyright © 2013 John Wiley & Sons, Ltd.
Identification of impurities in methotrexate drug substances using high‐performance liquid chromatography coupled with a photodiode array detector and Fourier transform ion cyclotron resonance mass spectrometry
Authors: Wu Cai‐Sheng , Tong Yuan‐Feng , Wang Peng‐Yuan , Wang Dong‐Mei , Wu Song , Zhang Jin‐Lan 
Published: April 2013

RATIONALEMethotrexate (MTX) is an antineoplastic therapeutic medicine that acts as an antimetabolite of folic acid. In this study we identified the impurities in MTX drug substances produced by different manufacturers and in different batches using high‐performance liquid chromatography coupled with a photodiode array detector and Fourier transform ion cyclotron resonance mass spectrometry (HPLC‐PDA/FTICR‐MS).METHODSMTX and its impurities were separated on a Restek Pinnacle II C18 column (250 × 4.6 mm, 5 µm) with a gradient elution system composed of 0.2% formic acid and acetonitrile at a flow rate of 1.0 mL/min. Ultraviolet (UV) detection was set at 305 nm. Mass detection was carried out using FTICR‐MS with full‐scan mass analysis at a resolving power of 100 000 coupled with multiple‐stage mass analysis using a parent list of compounds.RESULTSFifteen impurities were detected in MTX drug substances, and their structures were predicted from using HPLC‐PDA/FTICR‐MS data, including their UV spectra, high‐resolution mass spectrometry (HRMS), fragmentation patterns, and MSn spectra. Ten of the impurities detected in the MTX drug substances are reported for the first time. There was a high abundance of esterified impurities in some batches of MTX drug substances, over the identification threshold of International Conference on Harmonization (ICH) guidelines, which requires particular attention.CONCLUSIONSThis paper describes a HPLC‐PDA/FTICR‐MS method to profile and identify impurities in MTX drug substances. The results suggest that HPLC‐PDA/FTICR‐MS is a valuable analytical technique for the rapid identification of impurities. Copyright © 2013 John Wiley & Sons, Ltd.
Properties of isotope patterns and their utility for peptide identification in large‐scale proteomic experiments
Authors: Okawa Satoshi , Fischer Bernd , Krijgsveld Jeroen 
Published: April 2013

RATIONALEIn proteomic experiments, isotope patterns are routinely generated for all detected peptides. While this pattern is determined by peptide composition, it has not been evaluated as a parameter that can help in the process of peptide identification.METHODSFirst, we investigated how the relative isotope abundance (RIA) accuracy in proteomic data sets depends on the spectral intensity, resolution, and the number of mass spectrometry (MS) 1 scans, using an Orbitrap Velos mass spectrometer. Next, we explored the discriminatory power of isotope patterns in the context of proteome analyses of various complexities, either alone or in combination with a Mascot database search. Finally, we provide a theoretical framework for the required accuracies of both peptide mass and RIA for peptide identification.RESULTSWe demonstrate that the RIA error obtained in routine proteome analyses is 4–5%, and that this is only modestly influenced by spectral intensity, resolution, and the number of MS1 scans. While RIA alone has no discriminatory power, in a Mascot search isotope patterns can distinguish top scoring hits from runner‐up hits in 70–95% of cases. Our theoretical approach shows that RIA accuracy needs to be ~0.2% in order to uniquely identify peptides in full proteomes.CONCLUSIONSOur results demonstrate that isotope patterns can have discriminatory power when used in combination with a classical database search. Inclusion of this parameter in proteomic workflows may help to increase confidence in peptide identification, but in practical terms this will be limited to small proteomes. Copyright © 2013 John Wiley & Sons, Ltd.
Sensitivity in statistical evaluation of biomarkers
Authors: Li Wen , Qu Yongming , Kulkarni Pandurang M. 
Published: April 2013

AbstractIn clinical research and practice, biomarkers help in understanding disease progression and are useful when monitoring patients and making treatment decisions. Correct quantification of biomarkers in predicting the clinical outcome is important for better treatment of individual patients. Despite the rich literature in statistical validation, application of these validation methods in real data is not well studied. Specifically, the question is whether the change in a biomarker or the actual assessed value of the biomarker should be used. Contrary to most published papers in which the actual value of the biomarker is used, we showed through theory, simulation, and an example that it is more appropriate to use the change or the actual value in the marker with adjustment for the baseline value in evaluating a marker. Copyright © 2013 John Wiley & Sons, Ltd.
Microstructure of Nb‐Bearing Pipeline Steel with Improved Property Applying Ultrafast Cooling Process
Authors: Yao Sheng‐Jie , Du Lin‐Xiu , Wang Guo‐Dong 
Published: April 2013

AbstractX70 pipe line steel with high mechanical strength and high ductility can be achieved through hot rolling and ultrafast cooling process at a low cost. A large amount of nano‐sized precipitates and dislocations are identified near the surface layer of the steel applying ultrafast cooling, which contribute immeasurably to the improvement of mechanical properties. In addition, high cooling rate decreases the mean distance of pearlite laminae, and massive ferrite grains characterized by curved grain boundaries and high dislocation density are also transformed as one of the typical microstructures in the samples applying ultrafast cooling process.
Market Entry Strategies in Emerging Markets: An Institutional Study in the BRIC Countries
Authors: Holtbrügge Dirk , Baron Anastasia 
Published: April 2013

AbstractThis article addresses two of the strategically important questions of market entry strategies in emerging markets: how to enter and when to enter. Furthermore, we investigate the relationship between market entry strategies and market success. On the basis of the institutional theory, we develop hypotheses that are tested against data collected from 564 foreign firms with operations in the BRIC countries (Brazil, Russia, India, and China), using an online survey. Our findings suggest that market entry strategies have a significant effect on market success, showing the greatest market success in India and the lowest in China. We also find that entry mode and length of operation exhibit a significant positive effect on market success. © 2013 Wiley Periodicals, Inc.
FDI Inflow and Human Development: Analysis of FDI's Impact on Host Countries' Social Welfare and Infrastructure
Authors: Lehnert Kevin , Benmamoun Mamoun , Zhao Hongxin 
Published: April 2013

AbstractWe examine the impact of foreign direct investment (FDI) and the mediating effects of country national governance on the welfare and knowledge infrastructure of host countries. Based on a five‐year anchored panel data of 175 countries producing over 9,000 observations, we find that in general FDI has a positive influence on both host country welfare and knowledge infrastructure and the national governance positively mediates these relationships.1 © 2013 Wiley Periodicals, Inc.
Relationship Between Competitive Intensity, Internal Resources, and Firm Performance: Evidence from Indian ITES Industry
Authors: Lahiri Somnath 
Published: April 2013

AbstractThe purpose of this article is to investigate how competitive intensity impacts the relationship between crucial firm resources (human capital, organizational capital, management capability) and firm performance. Using a sample of 105 service providers from the Indian information technology–enabled services (ITES) industry, I find that competitive intensity positively moderates the relationship between firm resources and firm performance such that the relationships become stronger when competitive intensity is high than when it is low. Results imply that top managers' evaluation of the performance implications of internal firm resources are significantly shaped by the perceptions of intensity of competition encountered by their firms. © 2013 Wiley Periodicals, Inc.
Does 17‐α‐hydroxyprogesterone caproate affect fetal biometry and birth weight in twin pregnancy?
Authors: Mulder E. J. H. , Versteegh E. M. J. , Bloemenkamp K. W. M. , Lim A. C. , Mol B. W. J. , Bekedam D. J. , Kwee A. , Bruinse H. W. , Christiaens G. C. M. L. 
Published: April 2013

ABSTRACTObjectiveIncreasingly, maternal administration of 17‐α‐hydroxyprogesterone caproate (17‐OHPC) is utilized to prevent preterm birth, but the fetal safety of 17‐OHPC is still a matter of concern. This study aimed to assess whether exposure to 17‐OHPC during the second and third trimesters of pregnancy affects fetal biometry in twin gestations.MethodsThis study included a subset of women with a twin pregnancy who had been previously included in a randomized clinical trial comparing the effectiveness of 17‐OHPC and placebo on neonatal outcomes and preterm birth rates in multiple pregnancy. In the present study, the individual growth patterns of femur length, head circumference and abdominal circumference were compared between fetuses of women who had been randomized to receive weekly injections of either 17‐OHPC (n = 52) or placebo (n = 58) at between 16–20 and 36 weeks' gestation.ResultsThe three biometric variables assessed developed similarly in fetuses in both the group exposed to 17‐OHPC and the placebo group during the second half of pregnancy. Birth weight adjusted for parity and fetal sex was also comparable between groups.ConclusionThe use of 17‐OHPC has no adverse effects on fetal biometry and birth weight in twins. Copyright © 2013 ISUOG. Published by John Wiley & Sons Ltd.
Short term chemotherapy followed by radiofrequency ablation in stage III pancreatic cancer: results from a single center
Authors: Frigerio Isabella , Girelli Roberto , Giardino Alessandro , Regi Paolo , Salvia Roberto , Bassi Claudio 
Published: April 2013

AbstractBackgroundNeo‐adjuvant chemotherapy (CHT) has gained increasing importance in resectable and borderline resectable pancreatic cancer leading to a better performing surgery when we look at negative resection margins and selection of patients with less aggressive disease. We apply this principle to patients with Stage III (LAC) pancreatic cancer undergoing RFA and try to select patients who may benefit from a local treatment.MethodsAll patients affected by LAC were treated with RFA for a stable disease after a short CHT. Postoperative morbidity and mortality were evaluated together with overall survival (OS) and disease specific survival (DSS).ResultsWe consecutively treated 57 patients affected by LAC. Median duration of CHT before RFA was 5 months. The postoperative mortality rate was zero. Overall morbidity was 14 % with RFA‐related morbidity of 3.5 %. The OS and DSS were 19 months and when compared to a similar population who received RFA as up front treatment, there was no difference.ConclusionsOur results do not support the adoption of a short CHT as a way to identify patients to treat with RFA with the most benefit. Based on this and by knowing the role of immune modulation after RFA and its specific involvement in pancreatic carcinoma, we can propose RFA as upfront treatment.