bluuebunny/crossref_metadata_2025_split huggingface dataset:
Minimum Pulse Rate During Sleep: A Potential Non-Invasive Biomarker for Subtle Abnormalities in Mini-Mental State Examination from an Exploratory Cross-Sectional Multifaceted Survey in Active Older Adults
Authors: Tanaka Yuji , Saito Kozo , Tsuchiya Kyoichiro , Iwata Yusuke , Ando Takashi , Mochizuki Kazuki , Taniguchi Tamami , Mitsui Takahiko 
Published: July 2024

Abstract

Purpose
Developing quantitative indicators of daily life that can detect subtle cognitive decline is a significant challenge in the growing population of older adults worldwide. In this multifaceted survey conducted on active older adults, we aimed to explore novel indicators associated with subtle abnormalities in brief dementia screening tests.
Methods
Data were collected from 35 older adults who were not certified for long-term care or diagnosed with cognitive impairment using questionnaires, the Mini-Mental State Examination (MMSE), body composition measurements, sleep apnoea testing, activity monitoring, motor function assessments, blood tests, and nutrient analyses. Of the 89 factors examined in this study, several less invasive indicators for cognitive impairment were identified using Spearman’s correlation analysis, two-group comparison, and multiple linear regression model analysis.
Results
An elevated minimum pulse rate during sleep emerged as the most significant non-invasive marker correlated with both MMSE scores and the classification of cognitive impairment risk (mild cognitive impairment or dementia).
Conclusion
These findings could expedite further research into early cognitive decline detection among older adults and facilitate early intervention.
Implementation of an intelligent drinking water supply system using GIS mapping and smart metering for reliable water supply management
Authors: Pongiannan R. K. , Brindha R. , Geetha A. , Ganesan K. , JayeKumar M. , Maddileti Telugu , Preethivarshni K. 
Published: July 2024

ABSTRACT

The demand for water resources has increased due to population growth and the effects of cyclical droughts on irrigated agriculture. Due to these current circumstances, there is an imbalance between the limited supply of water and the rising demand for water. According to this perspective, accurate data on the spatial and temporal patterns of stockholder water demand can only be obtained through effective water planning and management. Geographic information system (GIS) mapping and smart metering are being used to implement intelligent water supply systems for dependable water supply management. In water supply systems, GIS models aid in data comprehension, analysis, and querying using advanced technologies that can significantly enhance work in the field of urban planning.
Evaluation of sediment generation and transport: a case study of Thwake Dam in Kenya
Authors: Maiyo Gilbert , Ndiba Peter Kuria , Odira Patts M. , Nyangeri Ezekiel 
Published: July 2024

ABSTRACT
The study aimed to evaluate sediment generation, transport, and deposition into the Thwake reservoir. This research sought to assess sediment transport patterns and their potential impact on the reservoir using regional and numerical techniques. The Thwake River basin constitutes 30% of the dam's catchment area and experiences high soil-loss due to its semi-arid climate, steep slopes, and lack of vegetation. The river system in the sub-basin is ephemeral, with the riverbed remaining dry throughout most of the year and experiencing tidal flow only during storm events. Bed-material samples were collected from selected reaches, and sediment properties were evaluated. The study involved analyzing datasets on the reservoir, catchment, and sand-bed channel. Numerical models assessed hydrological and sediment transport information by considering interacting variables and predicting deposition patterns and sediment-yield estimates. The findings indicated that sufficient bed material from sub-basin 3E would be deposited into the reservoir, resulting in delta formation approximately 5 km downstream of the tail waters at minimum dam operating level. The mass cumulative sediment inflow from 3E into the reservoir was estimated as between 14 and 26.3 metric tons per annum, representing reservoir loss and useful life under 50 years.
MODCEL-MHUS: a comprehensive multilayer hydrodynamic unified simulation for stormwater, sanitary sewer systems, and urban surface
Authors: de Oliveira Antonio Krishnamurti Beleño , Magalhães Paulo Canedo de , Rezende Osvaldo Moura , Sousa Matheus Martins de , Marques Rodrigo Hoerner Morais , Gomes Miguez Marcelo 
Published: July 2024

ABSTRACT

Numerous countries and regions have embraced implementing a separate sewer system, segregating sanitary and storm sewers into distinct systems. However, the functionality of these systems often needs to improve due to irregular interconnections, resulting in a mixed and malfunctioning system. Sewage collection is crucial for residential sanitation, but untreated collection significantly contributes to environmental degradation. Analyzing the simultaneous operation of both systems becomes vital for effective management. Using mathematical tools for precise and unified diagnosis and prognosis becomes imperative. However, municipal professionals and companies need more tools specifically designed to evaluate these systems in a unified way, mapping all the hydraulic connections observed in practice. This study proposes a unified simulation method for stormwater and sanitary sewer urban systems, addressing real-world scenarios and potential interferences. The primary goal is to develop a simulation method for both systems, considering system interconnections and urban layouts, involving hydrodynamic and water quality simulations. The practical application of this method, the Multilayer Hydrodynamic Simulation Method (MODCEL-MHUS), successfully identifies issues in urban water networks and suggests solutions, making it a valuable tool for urban water management and environmental engineering professionals.
Zé do Caixão
Authors: Kaizer Felicíssimo Débora 
Published: July 2024

Este trabalho tem como objetivo analisar o tema da desumanização da mulher nos filmes “À meia-noite levarei sua alma (1964)” e “Esta noite encarnarei no teu cadáver (1967)” ambos dirigidos pelo cineasta José Mojica Marins. Para isso, o artigo inicialmente foca na construção histórica do corpo e do gênero, a fim de introduzir um debate sobre os papéis de gênero. Em seguida, é feita a análise dos filmes, contextualizando-os historicamente e abordando como eles incorporam ideias da cultura e do imaginário brasileiro para tratar da violência contra a mulher no enredo.
A Rapid, Sensitive, and High-throughput Method for the Simultaneous Determination of Antihypertensive Drug Combinations in Dog Plasma by UHPLC-MS/MS: The Assessment of Predicable Bioequivalence of In-vitro Dissolution Condition
Authors: Wang Yuanjian , Wang Ruixun , Wang Huijia , Liu Ran , Bi Kaishun , Li Qing 
Published: July 2024

Background:
Essential hypertension is a common clinical disease and a risk factor for cardiovascular
and cerebrovascular diseases. Olmesartan medoxomil, amlodipine, and hydrochlorothiazide are commonly
used antihypertensive drugs. The aim of this study was to establish a robust UPLC-MS/MS method for the simultaneous
determination of olmesartan medoxomil, amlodipine, and hydrochlorothiazide in dog plasma. At
the same time, the in vivo and in vitro release studies were conducted, and a preliminary in vitro-in vivo correlation
(IVIVC) evaluation was performed.

Methods:
The bioequivalence experiment was conducted with a double-crossed design. Three major components
were extracted and analyzed by UHPLC-MS/MS. With the MRM scan, olmesartan and amlodipine were
quantified by fragment conversion (m/z 447.10→190.10) and (m/z 408.95→294.00) under positive ESI mode,
while hydrochlorothiazide was quantified with fragment conversion (m/z 295.90→268.90) under negative ESI
mode. The in vitro release studies were performed using a USP paddle, and the dissolution medium was chosen
from pH 6.0 to pH 6.8 according to the BCS classification of compounds. The IVIVC was calculated using
the Wagner-Nelson equation.

Results:
The linear ranges of olmesartan, amlodipine, and hydrochlorothiazide in the plasma were 5.0-2500,
0.1-50, and 3.0-1500 ng/mL, respectively. All accuracies were within 3.8% of the target values, and the findings
revealed that intra-day and inter-day accuracies were less than 12.1%. Moreover, the recoveries exceeded
88.3%, the matrix effect tests were positive, and the stability tests were positive. With the establishment of correlation,
the distinguishable dissolution condition (pH 6.8) was selected as the predictable condition.

Conclusion:
The established method was suitable for the preclinical pharmacokinetic study of tripartite drugs
with strong specificity and high sensitivity. Through the evaluation of IVIVC, the connection between in vivo
and in vitro drug testing was initially established.
Low Expression of SCN4B Predicts Poor Prognosis in Non-small Cell Lung Cancer
Authors: Li Xia , Chen Weiwei , Jiang Shu , Zhang Lianlian , Huang Hua , Ji Yanan , Ni Qinggan , Ling Chunhua 
Published: July 2024

Background:
Sodium voltage-gated channel beta subunit 4 (SCN4B) plays a suppressive role in various tumors. However, the role of SCN4B in non-small cell lung cancer
(NSCLC) is not yet clear. This study aims to investigate the expression of SCN4B in NSCLC
patients and its correlation with prognosis.

Methods:
Firstly, the expression of SCN4B in non-small cell lung cancer (NSCLC) was analyzed using The Cancer Genome Atlas (TCGA) database. Then, differential expression genes (DEGs) were identified using R software. Next, DEG enrichment pathways were analyzed using the R package clusterPro-filer. Protein-protein interaction networks were revealed through STRING analysis. A heatmap showed significant differential expression of SCN4B. Further analysis included examining SCN4B expression in a pan-cancer context and its correlation with 24 types of immune cells in NSCLC. Subsequently, quantitative real-time polymerase chain reaction (qRT-PCR), Western Blot, immunohistochemistry, and clinical data were used to validate SCN4B expression and prognostic value in NSCLC patients.

Results:
SCN4B mRNA expression in non-small cell lung cancer tissues was significantly lower than in adjacent normal tissues (p < 0.001). Clinical correlation analysis confirmed its association with clinical pathology. Gene set enrichment analysis (GSEA) and tumor immune-related analyses indicated that SCN4B is involved in NSCLC-related Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and participates in immune infiltration. qRT-PCR, Western Blot, and immunohistochemistry also con-firmed that SCN4B is downregulated in NSCLC patients and is associated with poor prognosis.

Conclusion:
SCN4B is downregulated in tumor tissues of NSCLC patients and is associated with a poor prognosis.
The Role of Epigenetic Mechanisms in the Treatment of GI Cancers
Authors: Shahini Ali , Imani Amirhossein , Reyhani Amirali , Khalili-Tanha Ghazaleh , Ghorbani Elnaz , Fiuji Hamid , Saeed Al-Hayawi Ibrahim , Khazaei Majid , Hassanian Seyed Mahdi , Ferns Gordon A. , Nazari Elham , Avan Amir 
Published: July 2024

Abstract:
Epigenetic mechanisms have been shown to play a critical role in the development and
progression of gastrointestinal [GI] cancers. These mechanisms involve modifications to DNA
and histones that can alter gene expression patterns and may contribute to the initiation and progression
of cancers. In recent years, epigenetic therapies have emerged as a promising approach to
treating GI cancers. These therapies target specific epigenetic modifications, such as DNA methylation
and histone acetylation, to restore normal gene expression patterns and inhibit cancer cell
growth. Several epigenetic drugs have been approved for the treatment of GI cancers. Moreover,
the use of epigenetic therapies in combination with other treatments, such as chemotherapeutic
agents, is being studied to improve treatment outcomes.
We have provided an overview of the role of epigenetic mechanisms in GI cancer treatment aimed
to focus on recent evidence of the use of epigenetic agents in clinical and preclinical GI cancer
studies, including gastric, esophageal, hepatic, pancreatic, and colorectal cancers. Overall, the role
of epigenetic mechanisms in GI cancer treatments is an active area of research with the potential
to improve patients' treatment outcomes and advance cancer treatment strategies.
The Search for Drugs Derived from Natural Products for Toxoplasma gondii Infection Treatment in the Last 20 Years - A Systematic Review
Authors: Marques-Santos Fabielle , Faria Robson Xavier , Amendoeira Maria Regina Reis 
Published: July 2024

Introduction:
Toxoplasmosis is a worldwide distributed zoonosis caused by Toxo-plasma gondii (T. gondii), an obligate intracellular protozoan. The infection in immunocompetent hosts usually progresses with mild or no symptoms. However, in immunocompromised individu-als, this disease can cause severe or fatal symptoms.

Method:
Sulfadiazine and pyrimethamine are two drugs used as standard therapies for human toxoplasmosis. Although they do not cause chronic infection, they may cause hematological tox-icity, hypersensitivity, intolerance, teratogenic effects, gastrointestinal disorders, and bone mar-row suppression.

Results:
The limited effect, significant toxicity, and emerging resistance to current drugs availa-ble to treat T. gondii infections require investigating other effective, nontoxic, and well-tolerated alternatives. Medicinal plants are, traditionally, the most promising sources used to treat infec-tious diseases.

Conclusion:
This review provides data on new therapeutic and prophylactic methods for T. gondii infection based on the use of extracts and/or compounds derived from natural products, which have been reported to be useful as alternative treatment options in the last 20 years.
When It Rains It Pours...: Wernicke Encephalopathy with Sudden
Blindness after Bariatric Surgery in a Patient with Congenital Deafness
Authors: Lopes Mariarita , Oteri Vittorio , Sceusa Giulia , Federica Spitali , Salvatore Volpe , Gullo Damiano 
Published: July 2024

Background::
Malabsorption syndromes are known chronic complications of bariatric
surgery. Therefore, it is recommended to take oral supplementation with multivitamins.
Wernicke's encephalopathy represents an acute neuropsychiatric syndrome associated with
alcoholism or severe malnutrition; sporadic cases of this potential complication related to bariatric
surgery are described in the literature. We present a case of Wernicke's encephalopathy due
to severe vitamin B1 deficiency after bariatric surgery.

Case Report::
A 31-year-old woman with deaf-mutism from the age of 3 years old, operated 3
months before with a mini-gastric bypass for severe obesity, was transferred to our unit after
accessing the emergency room. In the immediate medical history, there was the sudden and rapid
decline in vision, leading to complete loss of vision, marked asthenia, and paresthesia in the
four limbs. Considering the previous bariatric surgery, the diagnosis of non-alcoholic Wernicke's
syndrome was suspected, for which IV therapy with Vitamin B1 was started at a dosage
of 5 vials of 200 mg in 100 cc of saline solution (three times a day for the first 72 hours,
subsequently 1 once/day). After 12 hours, there was an improvement in visual acuity, and the
symptoms completely resolved within 48 hours. She was discharged with complete resolution of
all symptoms after 1 month.

Conclusion::
Initial vision loss without confusion or encephalopathy is one atypical presentation
of Wernicke syndrome. Clinical suspicion must be high in case of alcoholism or post-bariatric
surgery. Early recognition of atypical symptoms, including vision loss, and timely administration
of therapy improves the prognosis of this potentially reversible but time-dependent neurological
emergency.
Use of Web-Based Surveys to Collect Long-Term Pediatric Outcomes in Patients With Twin-Twin Transfusion Syndrome Treated With Fetoscopic Laser Photocoagulation: Observational Study
Authors: Bergh Eric , Rennie Kimberly , Espinoza Jimmy , Johnson Anthony , Papanna Ramesha 
Published: July 2024

Abstract

Background
In the United States, patients with monochorionic diamniotic twins who undergo in utero fetoscopic laser photocoagulation (FLP) for twin-twin transfusion syndrome (TTTS) may travel great distances for care. After delivery, many parents cannot return to study sites for formal pediatric evaluation due to geographic location and cost.

Objective
The aim of this study was to collect long-term pediatric outcomes in patients who underwent FLP for TTTS.

Methods
We assessed the feasibility of using a web-based survey designed in REDCap (Research Electronic Data Capture; Vanderbilt University) to collect parent-reported outcomes in children treated for TTTS at a single center during 2011‐2019. Patients with ≥1 neonatal survivor were invited via email to complete 5 possible questionnaires: the child status questionnaire (CSQ); fetal center questionnaire (FCQ); Ages & Stages Questionnaires, Third Edition (ASQ-3); Modified Checklist for Autism in Toddlers, Revised With Follow-Up (M-CHAT-R/F); and thank you questionnaire (TYQ). The R programming language (R Foundation for Statistical Computing) was used to automate survey distribution, scoring, and creation of customized reports. The survey was performed in 2019 and repeated after 12 months in the same study population in 2020.

Results
A total of 389 patients in 26 different states and 2 international locations had an email address on file and received an invitation in 2019 to complete the survey (median pediatric age 48.9, IQR 1.0‐93.6 months). Among surveyed mothers in 2019, the overall response rate was 37.3% (145/389), and the questionnaire completion rate was 98% (145/148), 87.8% (130/148), 71.1% (81/100), 86.4% (19/22), and 74.3% (110/148) for the CSQ, FCQ, ASQ-3, M-CHAT-R/F, and TYQ, respectively. In 2020, the overall response rate was 57.8% (56/97), and the questionnaire completion rate was 96.4% (54/56), 91.1% (51/56), 86.1% (31/36), 91.7% (11/12), and 80.4% (45/56) for the CSQ, FCQ, ASQ-3, M-CHAT-R/F, and TYQ, respectively.

Conclusions
This is the first study to use both REDCap and computer automation to aid in the dissemination, collection, and reporting of surveys to collect long-term pediatric outcomes in the field of fetal medicine.
Detection, Optimization, Characterization, and Cytotoxic Effect of Vaginolysin Extracted from Gardnerella Vaginalis Isolated from Iraqi Women Who Had Abortions
Authors: Najim Saja Ayad , Hussain Suzan Saadi , Abdulrazaq Raghad Abdulatef 
Published: July 2024

Objective: The goal of this study is the detection of the gen that responsible for production the vaginolysin toxin, discovered the optimal condition for produce vaginolysin, purification and characterization of the toxin and show the cytotoxic effect of vaginolysin on VK2cell and HeLa cell.
Methods: Among 592 specimens isolated from pregnant women suffering from vaginal inflammation and threatened with abortion(these women is suffering from strong pain in the cervical region), only 62 of isolates identified as G vaginalis. From Baghdad teaching hospital. detection of the gen that responsible for production the vaginolysin toxin. study of the optimal condition to produce vaginolysin. the purification of the toxin by using ammonium sulfate, ion exchange chromatography and gel filtration, toxin characterization of pH and temperature activity and stability, the study of cytotoxic effect of vaginolysin on VK2 and HeLa cells.
Result: The optimal condition to produce the toxin is pH level in 4, best temperature is 37 0C, the best media is TSB broth , and the best hours is 24hr. .
The best range for precipitation saturation ratio 50%. ion exchanges have specific activity 4 mg. gel filtrations show an increase in the specific activity of purified toxin (10U/mg).
the characterization done by pH and temperature,Vaginolysin was active in 0.5 U/ mL at pH 7, and stable in pH 7 activity, the best temperature for vaginolysin activity is 0.5 U/mL at 37°C and maximum stability was recorded at 37°C .
the cytotoxic effect of the toxin on VK2 cell observed the viability ratio 69.98%, at higher toxin concentration, while the result for HeLa cell viability ratio 38.77%, at higher toxin concentration.
Conclusion: vaginolysin cause lysis of vaginal cell and cervical cell may lead to abortion, the killing ratio increase by increasing the concentration of the toxin and the effect of the toxin on HeLa cell is more than on VK2 cell.
Pengaruh komik pancasila berbantuan metode role playing terhadap pemahaman nilai-nilai pancasila di sekolah dasar
Authors: Musqiroh Lita 
Published: July 2024

Penelitian ini bertujuan untuk mengetahui pengaruh media buku Komik Pancasila berbantuan metode Role Playing terhadap pemahaman nilai-nilai Pancasila. Penelitian ini dilaksanakan di SDN Sukamenak Indah pada bulan Februari-Maret 2023. Metode penelitian yang digunakan pada penelitian ini adalah pre eksperimen dengan design one group pretest-posttest design. Pengambilan sampel dilakukan dengan menggunakan teknik nonprobability sampling. Sampel penelitian yaitu 27 orang peserta didik kelas V. Instrumen yang digunakan dalam penelitian ini berupa soal tes pilihan ganda dan dokumentasi untuk mengamati kegiatan proses pembelajaran. Teknik pengolahan data yang digunakan yaitu uji validitas dan reliabilitas. Teknik analisis data yang digunakan dalam penelitian ini dengan uji normalitas yang menggunakan uji Liliefors dengan teknik Shapiro Wilk, uji homogenitas dengan menggunakan levenes statistic. Kemudian dilanjutkan dengan uji hipotesis menggunakan Independent samples T-Test. Setelah dilakukan pengujian dapat diperoleh thitung sebesar 3,720. Sedangkan ttabelÂ 2,036. Dengan kata lain thitung > ttabel. Maka dapat disimpulkan bahwa terdapat pengaruh penggunaan media buku Komik Pancasila berbantuan metode Role Playing terhadap pemahaman nilai-nilai Pancasila di kelas V SDN Sukamenak Indah.
Patient’s Satisfaction Post-Transurethral Resection of the Prostate with Monopolar and Bipolar for Benign Prostate Hyperplasia
Authors: Duarsa Gede Wirya Kusuma , Pradiptha I Nyoman Tri , Sugianto Ronald , Mahadewa Tjokorda Gde Bagus , Prakoeswa Cita Rosita Sigit 
Published: July 2024

Transurethral resection of the prostate (TURP), either bipolar or monopolar, is a surgical treatment for benign prostatic hyperplasia, which has similar effectiveness in reducing international prostate symptom score, increasing men peak flow rates, and reducing post-void residual volume. However, bipolar TURP (B-TURP) can reduce the incidence of bleeding and dilutional hyponatremia. This study aims to evaluate the effectiveness of the level of patient satisfaction between Monopolar-TURP (M-TURP) and B-TURP. 82 patients at three hospitals in Denpasar City were eligible as subjects based on inclusion criteria, consisting of 41 patients for each procedure. The recruitment method used consecutive sampling from March to August 2019. The patient’s demographics as baseline data were recorded. The level of patient satisfaction was recorded for one month after surgery through the SF-36 questionnaire. The baseline sample’s characteristics before surgery in both groups were statistically identical. In evaluating patient satisfaction levels, there are significant differences where TURP (B-TURP) is better in the domain of vitality (p=0.001), social function (p=0.005), pain (p=0.002), and general health (p=0.001). B-TURP resulted in an increasing level of patient satisfaction better than M-TURP.