bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of an extruded micronutrient delivery vehicle for the fortification of nixtamalised maize with chelated iron at the point of wet milling
Authors: Torres Pablo C. , Flores Fátima E. , Andrade Juan E. 
Published: April 2015

SummaryThe use of high‐temperature extrusion for the development of a micronutrient delivery vehicle (MDV), made of rice and maize (1:1 w/w), as part of a fortification technology for traditional nixtamalised maize (NM) masa at the point of use was evaluated. A Welly puffing extruder and a peristaltic pump were used to establish a uniform extrusion process for MDVs containing 302 ± 13 mg kg−1 of NaFeEDTA or 656 ± 8 mg kg−1 of ferrous bisglycinate. After manual premixing (30 s) of MDVs with NM (1:20 or 1:40 MDV:NM) and grinding with a pilot scale burr mill (5 min), iron distribution in masa was variable, ranging 3–21% RSD within sections of individual 2‐kg batches. Kneading (5 min) shortly after grinding resulted in uniformed iron distribution (<4% RSD). Despite the iron source and fortification level, fortified NM masa was harder and darker than control after 8‐h storage at room temperature; however, these changes require further sensory evaluation.
Changes in the physical properties of honey powder during storage
Authors: Samborska Katarzyna , Langa Elwira , Bakier Sławomir 
Published: April 2015

SummaryHoney powders produced by spray drying with the addition of Arabic gum and sodium caseinate were characterised in terms of the influence of storage time on the following physical properties: particle size, moisture content, water activity, bulk density, flowability and hygroscopicity. The storage affected those properties; after 12 weeks of storage, particle size decreased (except Arabic gum powder treated as a control sample), moisture content and water activity increased, hygroscopicity decreased. Changes in bulk density, particle size and moisture content caused the rise of Hausner ratio value; however, the powders were still characterised by a very good flow properties and low cohesiveness. The colour of reconstituted powders was also affected by storage; in most of samples, the darkening, reduction in redness and yellowness were observed. Colour parameters were the most stable in powders obtained with the addition of 2% w/w of sodium caseinate.
Tetrahymena Expresses More than a Hundred Proteins with Lipid‐binding MORN Motifs that can Differ in their Subcellular Localisations
Authors: Habicht Jörn , Woehle Christian , Gould Sven B. 
Published: April 2015

AbstractProteins with membrane occupation and recognition nexus (MORN) motifs are associated with cell fission in apicomplexan parasites, chloroplast division in Arabidopsis and the motility of sperm cells. We found that ciliates are among those that encode the largest variety of MORN proteins. Tetrahymena thermophila expresses 129 MORN protein‐encoding genes, some of which are specifically up‐regulated during conjugation. A lipid‐binding assay underpins the assumption that the predominant function of MORN motifs themselves is to confer the ability of lipid binding. The localisation of four MORN candidate proteins with similar characteristics highlights the functional diversity of this group especially in ciliates.
Identification and fine mapping of quantitative trait loci for the number of vascular bundle in maize stem
Authors: Huang Cheng , Chen Qiuyue , Xu Guanghui , Xu Dingyi , Tian Jinge , Tian Feng 
Published: April 2015

AbstractStudies that investigated the genetic basis of source and sink related traits have been widely conducted. However, the vascular system that links source and sink received much less attention. When maize was domesticated from its wild ancestor, teosinte, the external morphology has changed dramatically; however, less is known for the internal anatomy changes. In this study, using a large maize‐teosinte experimental population, we performed a high‐resolution quantitative trait locus (QTL) mapping for the number of vascular bundle in the uppermost internode of maize stem. The results showed that vascular bundle number is dominated by a large number of small‐effect QTLs, in which a total of 16 QTLs that jointly accounts for 52.2% of phenotypic variation were detected, with no single QTL explaining more than 6% of variation. Different from QTLs for typical domestication traits, QTLs for vascular bundle number might not be under directional selection following domestication. Using Near Isogenic Lines (NILs) developed from heterogeneous inbred family (HIF), we further validated the effect of one QTL qVb9‐2 on chromosome 9 and fine mapped the QTL to a 1.8‐Mb physical region. This study provides important insights for the genetic architecture of vascular bundle number in maize stem and sets basis for cloning of qVb9‐2.
Lessons Learned from the Expansion of Naloxone Access in Massachusetts and North Carolina
Authors: Davis Corey S. , Walley Alexander Y. , Bridger Colleen M. 
Published: April 2015

States are rapidly modifying law and policy to increase access to the opioid antidote naloxone, and the provision of naloxone rescue kits (NRK) for use in the event of overdose is becoming increasingly common. As of late 2014 the majority of states had passed laws increasing naloxone access, and nearly as many have modified emergency responder scope of practice protocols to permit Emergency Medical Technicians (EMTs) and law enforcement officers to administer the medication. While the text of these laws is generally similar, their implementation varies among states.This article outlines experiences and lessons learned from two diverse states, Massachusetts and North Carolina. In Massachusetts naloxone access initiatives were well underway before formal legislative action occurred, while in North Carolina the passage of a naloxone access law served as a catalyst for the creation of new programs and facilitated the scale-up of existing ones. In both states legislative action was necessary to permit the prescription and dispensing of naloxone to the friends and family members of people who use opioids, a key legal change.
The effect of pre‐tectonic reaction and annealing extent on behaviour during subsequent deformation: insights from paired shear zones in the lower crust of Fiordland, New Zealand
Authors: Smith J. R. , Piazolo S. , Daczko N. R. , Evans L. 
Published: April 2015

AbstractGranulite facies pargasite orthogneiss is partially to completely reacted to garnet granulite either side of narrow (<20 mm) felsic dykes, in Fiordland, New Zealand, forming ~10–80 mm wide garnet reaction zones. The metamorphic reaction changed the abundance of minerals, and their shape and grain size distribution. The extent of reaction and annealing (temperature‐related coarsening and nucleation) is greatest close to the dykes, whereas further away the reaction is incomplete. As a consequence, grain size and the abundance of garnet decreases away from the felsic dykes over a few centimetres. The aspect ratios of clusters of S1 pyroxene and pargasite in the orthogneiss, which are variably reacted to post‐S1 garnet, decrease from high in the host, to near equidimensional close to the dyke. Post‐reaction deformation localized in the fine‐grained partially reacted areas. This produced a pattern of ‘paired’ shear zones located at the outer parts of the garnet reaction zone. Our study shows that grain size sensitive deformation occurs where the grain size is sufficiently reduced by metamorphic reaction. The weakening of the rock due to the change in grain size distribution outweighs the addition of nominally stronger garnet to the assemblage.
Total tract nutrient digestion and milk fatty acid profile of dairy cows fed diets containing different levels of whole raw soya beans
Authors: Venturelli B. C. , de Freitas Júnior J. E. , Takiya C. S. , de Araújo A. P. C. , Santos M. C. B. , Calomeni G. D. , Gardinal R. , Vendramini T. H. A. , Rennó F. P. 
Published: April 2015

SummaryWhole oilseeds such as soya beans have been utilized in dairy rations to supply additional fat and protein. However, antinutritional components contained in soya beans, such as trypsin inhibitors and haemagglutinins (lectins) may alter digestibility of nutrients and consequently affect animal performance. The objective of the present experiment was to quantify the effect of different levels of whole raw soya beans in diets of dairy cows on nutrient intake, total tract digestion, nutrient balances and milk yield and composition. Sixteen mid to late‐lactation cows (228 ± 20 days in milk; mean ± SD) were used in four replicated 4 × 4 Latin square design experiment with 21‐d periods. Cows were assigned to each square according to milk yield and DIM. The animals were randomly allocated to treatments: control (without soya beans addition; CO), WS9, WS18 and WS27, with addition of 9%, 18% and 27% of whole raw soya bean in diet on a dry matter (DM) basis respectively. All diets contained identical forage and concentrate components and consisted of maize silage and concentrate based on ground corn and soya beans at a ratio of 60:40. There were no differences in OM, CP, NDF and NEL intakes (kg/day and MJ/day) among the treatments (p > 0.05). However, DM and NFC intakes were negatively affected (p = 0.04 and p < 0.01, respectively) and ether extract (EE) intake was positively affected (p < 0.01). Total tract digestion increased linearly with whole raw soya beans for EE (p < 0.01) and NDF (p = 0.01). The excretion (kg/day) of digested soya beans grains increased linearly according to addition of whole raw soya beans. However, the nutritive characteristics of excreted grains were not altered. Milk (kg), milk lactose (kg) and protein (kg) yield decreased linearly (p < 0.01, p < 0.01 and p = 0.04, respectively) milk fat content (%) increased linearly (p < 0.01) with whole raw soya beans inclusion. Increasing addition of whole raw soya beans affected milk fatty acid profile with a linear decrease of cis‐9‐trans 11CLA and total saturated FA; and linear increase of total unsaturated and C18:3 FA. Energy balance was positively affected (p = 0.03) by whole raw soya beans as well as efficiency of NEL milk/DE intake (p = 0.02). Nitrogen balance and microbial protein synthesis were not affected by whole raw soya beans. Increasing doses of whole raw soya beans decreased dry matter intake and milk yield, however, led to an increase of unsaturated acids in milk and higher milk fat concentration.
Notational Variants and Invariance in Linguistics
Authors: Johnson Kent 
Published: April 2015

AbstractThis article argues that the much‐maligned ‘notational variants’ of a given formal linguistic theory play a role similar to alternative numerical measurement scales. Thus, they can be used to identify the invariant components of the grammar; i.e., those features that do not depend on the choice of empirically equivalent representation. Treating these elements as the ‘meaningful’ structure of language has numerous consequences for the philosophy of science and linguistics. I offer several such examples of how linguistic theorizing can profit from adopting a measurement‐theoretic viewpoint. The first concerns a measurement‐theoretic response to a famous criticism of Quine's. Others follow from issues of simplicity in the current biolinguistics program. An unexpected similarity with behaviorist practices is also uncovered. I then argue that managable and useful steps can be taken in this area.
Sensory Substitution is Substitution
Authors: Martin Jean‐Rémy , Le Corre François 
Published: April 2015

AbstractSensory substitution devices (SSDs) make use of one substituting modality (e.g. touch) to get access to environmental information normally accessed through another modality (e.g. vision). Based on behavioural and neuroimaging data, some authors have claimed that using a vision‐substituting device results in visual perception. Reviewing these data, we contend that this claim is untenable. We argue (i) that the kind of information processed by a SSD is metamodal, so that it can be accessed through any sensory modality and (ii) that the phenomenology associated with the use of a SSD is best described in terms of spatial phenomenology, only.
Aspergillus nidulans flippase DnfA is cargo of the endocytic collar and plays complementary roles in growth and phosphatidylserine asymmetry with another flippase, DnfB
Authors: Schultzhaus Zachary , Yan Huijuan , Shaw Brian D. 
Published: April 2015

SummaryEndocytosis and exocytosis are strictly segregated at the ends of hyphal cells of filamentous fungi, with a collar of endocytic activity encircling the growing cell tip, which elongates through directed membrane fusion. It has been proposed that this separation supports an endocytic recycling pathway that maintains polar localization of proteins at the growing apex. In a search for proteins in the filamentous fungus Aspergillus nidulans that possess an NPFxD motif, which signals for endocytosis, a Type 4 P‐Type ATPase was identified and named DnfA. Interestingly, NPFxD is at a different region of DnfA than the same motif in the Saccharomyces cerevisiae ortholog, although endocytosis is dependent on this motif for both proteins. DnfA is involved in asexual sporulation and polarized growth. Additionally, it is segregated within the Spitzenkörper from another Type 4 P‐type ATPase, DnfB. Next, the phosphatidylserine marker GFP::Lact‐C2 was expressed in growing hyphae, which revealed that this phospholipid is enriched on the cytosolic face of secretory vesicles. This distribution is affected by deleting either dnfA or dnfB. These findings provide evidence for the spatial and temporal segregation of Type4‐ATPases in filamentous fungi, and the asymmetric distribution of phosphatidylserine to the Spitzenkörper in A. nidulans.
A conserved proline residue in Dothideomycete Avr4 effector proteins is required to trigger a Cf‐4‐dependent hypersensitive response
Authors: Mesarich Carl H. , Stergiopoulos Ioannis , Beenen Henriek G. , Cordovez Viviane , Guo Yanan , Karimi Jashni Mansoor , Bradshaw Rosie E. , de Wit Pierre J. G. M. 
Published: April 2015

SummaryCfAvr4, a chitin‐binding effector protein produced by the Dothideomycete tomato pathogen Cladosporium fulvum, protects the cell wall of this fungus against hydrolysis by secreted host chitinases during infection. However, in the presence of the Cf‐4 immune receptor of tomato, CfAvr4 triggers a hypersensitive response (HR), which renders the pathogen avirulent. Recently, several orthologues of CfAvr4 have been identified from phylogenetically closely related species of Dothideomycete fungi. Of these, DsAvr4 from Dothistroma septosporum also triggers a Cf‐4‐dependent HR, but CaAvr4 and CbAvr4 from Cercospora apii and Cercospora beticola, respectively, do not. All, however, bind chitin. To identify the region(s) and specific amino acid residue(s) of CfAvr4 and DsAvr4 required to trigger a Cf‐4‐dependent HR, chimeric and mutant proteins, in which specific protein regions or single amino acid residues, respectively, were exchanged between CfAvr4 and CaAvr4 or DsAvr4 and CbAvr4, were tested for their ability to trigger an HR in Nicotiana benthamiana plants transgenic for the Cf‐4 immune receptor gene. Based on this approach, a single region common to CfAvr4 and DsAvr4 was determined to carry a conserved proline residue necessary for the elicitation of this HR. In support of this result, a Cf‐4‐dependent HR was triggered by mutant CaAvr4 and CbAvr4 proteins carrying an arginine‐to‐proline substitution at this position. This study provides the first step in deciphering how Avr4 orthologues from different Dothideomycete fungi trigger a Cf‐4‐dependent HR.
Asthma and allergy in children with and without prior measles, mumps, and rubella vaccination
Authors: Timmermann Clara Amalie Gade , Osuna Christa Elyse , Steuerwald Ulrike , Weihe Pál , Poulsen Lars K. , Grandjean Philippe 
Published: April 2015

AbstractBackgroundThe existing literature on the association between measles vaccination and subsequent risk of allergic disease is inconclusive. The aim of this study was, therefore, to determine whether measles, mumps, and rubella (MMR) vaccination administered in early childhood was associated with asthma and allergic diseases at ages 5, 7 and 13 yrs in a birth cohort.MethodsIn the Faroe Islands, 640 children were followed from birth. Follow‐up examinations at ages 5, 7 and 13 yrs included a physical examination and a maternal questionnaire about the child's health. At age 7, total and grass‐specific IgE was quantified in the child's serum, and at age 13, the children underwent skin prick tests (SPT). The child's vaccination card was reviewed at examinations.ResultsAt age 5, 533 of 555 children had been vaccinated for MMR. After confounder adjustment, we found early life MMR vaccination to be associated with a two‐third reduction in the odds of asthma (OR: 0.33, 95% CI: 0.12; 0.90) and hypersensitivity/allergy (OR: 0.32, 95% CI: 0.11; 0.88) at age 5, and the substantially decreased odds of asthma were replicated at age 13 (OR: 0.22, 95% CI: 0.08; 0.56). At age 7, serum total IgE was reduced by 62.8% (CI 95%: −84.3%; −11.9%) in the vaccinated children. MMR vaccination was not significantly associated with allergic rhinoconjuctivitis symptoms, eczema, or SPT reactions at age 13.ConclusionsMMR vaccination early in life may have a protective effect against allergy at least up to age 7 and against asthma through age 13 yrs.
Toward predicting metastatic progression of melanoma based on gene expression data
Authors: Li Yuanyuan , Krahn Juno M. , Flake Gordon P. , Umbach David M. , Li Leping 
Published: April 2015

SummaryPrimary and metastatic melanoma tumors share the same cell origin, making it challenging to identify genomic biomarkers that can differentiate them. Primary tumors themselves can be heterogeneous, reflecting ongoing genomic changes as they progress toward metastasizing. We developed a computational method to explore this heterogeneity and to predict metastatic progression of the primary tumors. We applied our method separately to gene expression and to microRNA (miRNA) expression data from ~450 primary and metastatic skin cutaneous melanoma (SKCM) samples from the Cancer Genome Atlas (TCGA). Metastatic progression scores from RNA‐seq data were significantly associated with clinical staging of patients’ lymph nodes, whereas scores from miRNA‐seq data were significantly associated with Clark's level. The loss of expression of many characteristic epithelial lineage genes in primary SKCM tumor samples was highly correlated with predicted progression scores. We suggest that those genes/miRNAs might serve as putative biomarkers for SKCM metastatic progression.
Police and Thieves in the Stadium: Measuring the (Multiple) Effects of Football Matches on Crime
Authors: Marie Olivier 
Published: April 2015

SummaryLarge sporting events affect criminal behaviour via three channels: fan concentration, self-incapacitation and police displacement. I exploit information on football matches for London teams linked to detailed recorded crime data at the area level to estimate these effects empirically. I find that only property crime increases in the communities hosting matches but not violent offences. There is a negative away game attendance effect on crime which is due to voluntary incapacitation of potential offenders attending a match. Police displacement during home games increases property crime by 7 percentage points for every extra 10000 supporters in areas that are left underprotected.
Beyond Completion Rate: Evaluating the Passing Ability of Footballers
Authors: Szczepański Łukasz , McHale Ian 
Published: April 2015

SummaryPassing the ball is one of the key skills of a football player yet the metrics commonly used to evaluate passing ability are crude and largely limited to various forms of a pass completion rate. These metrics can be misleading for two general reasons: they do not account for the difficulty of the attempted pass nor the various levels of uncertainty involved in empirical observations based on different numbers of passes per player. We address both these deficiencies by building a statistical model in which the success of a pass depends on the skill of the executing player as well as other factors including the origin and destination of the pass, the skill of his teammates and the opponents, and proxies for the defensive pressure put on the executing player as well as random chance. We fit the model by using data from the 2006–2007 season of the English Premier League provided by Opta, estimate each player's passing skill and make predictions for the next season. The model predictions considerably outperform a naive method of simply using the previous season's completion rate as a predictor of the following season's completion rate. In particular, we show how a change in the difficulty of passes attempted in both seasons explains a significant proportion of the shift in the observed performance of some players—a fact that is ignored if the raw completion rate is used to evaluate player skill.
Modelling the Type and Timing of Consecutive Events: Application to Predicting Preterm Birth in Repeated Pregnancies
Authors: Shih Joanna H. , Albert Paul S. , Mendola Pauline , Grantz Katherine L. 
Published: April 2015

SummaryPredicting the occurrence and timing of adverse pregnancy events such as preterm birth is an important analytical challenge in obstetrical practice. Developing statistical approaches that can be used to assess the risk and timing of these adverse events will provide clinicians with tools for individualized risk assessment that account for a woman's prior pregnancy history. Often adverse pregnancy outcomes are subject to competing events; for example, interest may focus on the occurrence of pre-eclampsia-related preterm birth, where preterm birth for other reasons may serve as a competing event. We propose modelling the type and timing of adverse outcomes in repeated pregnancies. We formulate a joint model, where types of adverse outcomes across repeated pregnancies are modelled by using a polychotomous logistic regression model with random effects, and gestational ages at delivery are modelled conditionally on the types of adverse outcome. The correlation between gestational ages conditional on the adverse pregnancies is modelled by the semiparametric normal copula function. We present a two-stage estimation method and develop the asymptotic theory for the estimators proposed. The model and estimation procedure proposed are applied to the National Institute of Child Health and Human Development consecutive pregnancies study data and evaluated by simulations.