bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single‐cell RNA sequencing reveals the CRTAC1+ population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1+ macrophage
Authors: Tang Yulong , Zhuo Dachun , Yu Yuexin , Pu Weilin , Ma Yanyun , Zhang Yuting , Huang Yan , Zhang Qing , Tang Kunhai , Meng Chen , Yang Di , Bai Lu , He Dongyi , Jin Li , Zou Hejian , Xu Huji , Zhu Qi , Wang Jiucun , Chen Yuanyuan , Liu Jing 
Published: August 2024

AbstractDegenerative spinal stenosis is a chronic disease that affects the spinal ligaments and associated bones, resulting in back pain and disorders of the limbs among the elderly population. There are few preventive strategies for such ligament degeneration. We here aimed to establish a comprehensive transcriptomic atlas of ligament tissues to identify high‐priority targets for pharmaceutical treatment of ligament degeneration. Here, single‐cell RNA sequencing was performed on six degenerative ligaments and three traumatic ligaments to understand tissue heterogeneity. After stringent quality control, high‐quality data were obtained from 32,014 cells. Distinct cell clusters comprising stromal and immune cells were identified in ligament tissues. Among them, we noted that collagen degradation associated with CTHRC1+ fibroblast‐like cells and calcification linked to CRTAC1+ chondrocyte‐like cells were key features of ligament degeneration. SCENIC analysis and further experiments identified ATF3 as a key transcription factor regulating the pathogenesis of CRTAC1+ chondrocyte‐like cells. Typically, immune cells infiltrate localized organs, causing tissue damage. In our study, myeloid cells were found to be inflammatory‐activated, and SPP1+ macrophages were notably enriched in degenerative ligaments. Further exploration via CellChat analysis demonstrated a robust interaction between SPP1+ macrophages and CRTAC1+ chondrocyte‐like cells. Activated by SPP1, ATF3 propels the CRTAC1/MGP/CLU axis, fostering ligament calcification. Our unique resource provides novel insights into possible mechanisms underlying ligament degeneration, the target cell types, and molecules that are expected to mitigate degenerative spinal ligament. We also highlight the role of immune regulation in ligament degeneration and calcification, enhancing our understanding of this disease.
Hierarchical drift‐diffusion modelling uncovers differences of valenced self‐evaluation
Authors: Wang Nan , Shi Kun , Li Jiwen , Chen Haopeng , Tang Jianchao , Liu Yadong , Zhao Xiaolin , Yang Juan 
Published: August 2024

AbstractDifferences in valenced self‐evaluation refer to positive and negative coexistence in the process of self‐evaluation, while there is a clear difference in cognitive processes. The present study aimed to uncover the differences in the latent cognitive parameters (e.g., processing speed) in valenced self‐evaluation using the hierarchical drift‐diffusion model in two independent experiments. A self‐referential decision‐making task was applied in both experiments, and a self‐descriptiveness task plus the rating of related emotions (e.g., pride and shame) were also used but only in Experiment 2. Results of Experiments 1 & 2 showed a faster processing speed for accepting positive attributes and longer times for encoding and response execution in negative self‐evaluation. Moreover, Experiment 2 found cognitive parameters had predictive effects on subsequent decisional outcomes such as self‐descriptiveness and self‐related emotions via Bayesian inference. The current study provided findings that help to understand the cognitive mechanism behind self‐positivity and self‐accuracy biases.
Assessment of demand for male contraceptives: A multi‐country study
Authors: Kaur Japneet , Rohini Dhananjay Vaidyanathan , Chang LinChiat , Gugliotti Allie , Kretschmer Steve 
Published: August 2024

AbstractBackgroundAs of 2019, high unmet contraceptive needs in low‐ and middle‐income countries result in nearly half of all pregnancies being unintended. Additional male contraceptive options could help, but gaps remain in assessing demand for them. As the development of novel male contraceptives progresses, addressing these gaps would help inform policy and scientific direction.ObjectivesMeasure consumer demand for novel male contraceptives and identify product attributes associated with men's preferences.Materials & methodsA cross‐sectional, probability‐based‐sample survey was conducted with 12,435 sexually active, fertile cisgender, heterosexual men aged 18–60 years, and 9,122 of their female partners in Bangladesh, Vietnam, Kenya, Nigeria, DR Congo, and Côte d'Ivoire from May 2021 to October 2022. The survey was also conducted in the United States among 3,243 of these men in April 2022. A follow‐up with 3,070 men in May 2023 assessed potential changes in demand after the Supreme Court's Dobbs v. Jackson Women's Health Organization decision. The surveys assessed contraceptive use, perceptions, and sociodemographic characteristics, and a discrete choice experiment evaluated preferences for 11 potential product attributes. Data from female partners in the six LMICs indicated their interest and trust in male partners’ contraceptive use.ResultsOn average, 61% of men showed interest in trying novel male contraceptives in their first year of availability, ranging from 39% and 49% (pre‐ and post‐Dobbs) in the United States to 76% in Nigeria and Bangladesh. Form of administration and time of use drove men's product preferences. Female partners’ interest and trust in male contraceptive use were high across geographies.DiscussionThis study reveals strong latent demand for novel male contraceptives, highlighting the potential for novel male methods to meet unmet needs, and female partners’ trust in men to use them.ConclusionHigh demand for novel male contraceptives among men and their partners exists, supporting investment in their development.
Jaw in a day surgery: early experience with 19 patients at an Australian tertiary referral center
Authors: Jeong Yu Jin , Dunn Masako , Manzie Timothy , Howes Dale , Wykes James , Palme Carsten E. , Leinkram David , Low Tsu‐Hui (Hubert) , Oberoi Ramman , Aung Yee Mon , Ormsby Christopher , Clark Jonathan 
Published: August 2024

AbstractBackgroundThe Jaw‐in‐a‐Day (JIAD) procedure aims to achieve immediate functional occlusion via a single‐stage approach to maxillofacial reconstruction. While JIAD has gained popularity since its inception by Levine and colleagues, efficacy and outcome data remain limited. In this report, we discuss our experience with the JIAD technique at an Australian tertiary referral centre.MethodsA retrospective review of all JIAD procedures performed from April 2022 to December 2023 was conducted. Clinicopathologic data reviewed included demographic information, primary diagnosis, anatomical site of disease, and history of pre‐operative radiotherapy. Outcome measures of interest included operative time, number of implants placed, post‐operative complications and implant survival.ResultsNineteen patients were identified for the study. Two maxillary and 17 mandibular JIAD procedures were performed. The most common indications were squamous cell carcinoma (n = 8) and ameloblastoma (n = 5). Surgical complications included recipient site wound infection (n = 3), flap dehiscence (n = 2), haematoma formation (n = 1), and neck abscess associated with partial flap failure (n = 1). No total flap failures were identified. Of the 55 total implants placed, one implant failure occurred 2‐months post‐operatively. No loss of irradiated implants (n = 21) was observed. The median time to adjuvant radiotherapy was 57 days (range, 32–61). Eighteen of 19 patients (95%) achieved immediate dental rehabilitation, and 15/19 patients (79%) retained a functional prosthesis by the end of the follow‐up period.ConclusionsOur series supports the feasibility of single‐stage reconstruction for both benign and malignant indications. Further research is required to understand the long‐term functional, aesthetic, and health‐related quality‐of‐life outcomes with the JIAD technique.
Promoting medication safety for older adults upon hospital discharge: Guiding principles for a medication discharge plan
Authors: Zhang Fang Hao , Lauzon Justine , Payette Jérémy , Courtemanche Fanny , Papillon‐Ferland Louise , Firoozi Faranak , Gilbert Suzanne , Turner Justin P. , Villeneuve Yannick 
Published: August 2024

AbstractOlder adults are at risk of adverse drug events during transition of care from hospital to community, thus optimal communication about medications at discharge is essential. Standardization of medication discharge plan (MDP) is lacking. This study aimed to (1) create a standardized MDP for older adults using consensus‐based principles, (2) create a short‐version MDP and (3) generate a practical guide. Modified Delphi was used to establish consensus on guiding principles for the MDP. Additionally, participants were asked about guiding principles deemed most essential, patient prioritization, the format and mode of transmission of the MDP. Twenty‐six guiding principles reached consensus, with 17 prioritized for a short‐version MDP. The practical guide includes explanations of the guiding principles, criteria for patient selection and recommendations on the format and mode of transmission. The results of this study will assist implementation of MDPs when older adults are discharged from hospital.
Opt‐out vaccination in school and daycare: Reconciling parental authority and obligations
Authors: Andersen Didde Boisen , Pedersen Viki Møller Lyngby 
Published: August 2024

AbstractAn increasing vaccine hesitancy among parents, which has resulted in insufficient rates of immunization, provides reason to reconsider childhood vaccination practices. Studies suggest that parents' decision‐making process concerning whether to vaccinate their child is highly influenced by cognitive biases. These biases can be utilized to increase vaccination uptake via changes in the choice context. This article considers childhood vaccination programmes, which involve children being vaccinated in school or daycare unless their parents actively ‘opt out’. We suggest that such programmes reconcile parents' decisional authority and vaccination duties. First, opt‐out childhood vaccination based in schools or daycare centres are not disrespectful of parental authority. Second, the programme aligns the default setting with a moral obligation to vaccinate one's child that most parents have.
Posttraumatic growth and its associations with perceived stress and core beliefs in women after traumatic childbirth during the COVID‐19 pandemic
Authors: Silva Rosa , Prata Ana Paula , Abreu Wilson , Brandão Tânia , Brandão Sónia , Riklikiene Olga , Jarasiunaite‐Fedosejeva Gabija , González Mesa Ernesto S. , Gökçe İsbir Gözde , Inci Figen , Kömürcü Akik Burcu , Uriko Kristiina , Thompson Gill 
Published: August 2024

AbstractBackgroundThe COVID‐19 pandemic added new challenges and stressors to the childbirth period, potentially increasing the risk of traumatic childbirth experiences. There is little known about posttraumatic growth (PTG) in a childbearing population. This study describes PTG in women after traumatic childbirth during the COVID‐19 pandemic and its association with sociodemographic, birth‐related characteristics, traumatic childbirth events, perceived stress, and core beliefs, as well as explores what factors predict PTG.MethodsA cross‐sectional study was conducted with 202 women who self‐identified as having experienced traumatic childbirth. Measures included sociodemographic and birth‐related characteristics, traumatic childbirth events, self‐reported stress during childbirth, the PTG Inventory, and the Core Beliefs Inventory (CBI).ResultsPerceived stress at the time of birth was very high in 70% of the respondents. CBI showed moderate disruption of core beliefs. 41.6% of mothers indicated substantial PTG. Education and type of birth were related to perceived stress levels; higher disruption of core beliefs was observed in individuals who experienced perineal trauma and lack of partners' presence during childbirth, and higher disruption of core beliefs was positively associated with PTG. Predictive models showed that perceived stress had a minimal effect, while the disruption of core beliefs showed a significant positive association with PTG.ConclusionTraumatic childbirth experiences during the COVID‐19 pandemic were positively related to PTG. Health professionals should create an environment where women can explore their feelings and emotions. Changes in current practices are also necessary as cesareans have been shown to be highly associated with high levels of perceived stress.
Association of paediatric autoimmune cytopenia and inflammatory bowel disease suggests a common genetic origin
Authors: Gilton M. , Fernandes H. , Martinez C. , Leverger G. , Abou Chahla W. , Li Thiao Te V. , Deparis M. , Armari Alla C. , Garnier N. , Benadiba J. , Marie‐Cardine A. , Rieux‐Laucat F. , Picard C. , Aladjidi N. , Leblanc T. 
Published: August 2024

SummaryThe association of autoimmune cytopenia (AIC) and inflammatory bowel disease (IBD) has been reported in small series, but the incidence of and risk factors for IBD in children with AIC are not known. One thousand six hundred nine children with chronic immune thrombocytopenic purpura, autoimmune haemolytic anaemia or Evans syndrome from the prospective OBS'CEREVANCE cohort are included in this study. Overall, 15 children were diagnosed with IBD, including 14 who developed IBD after AIC diagnosis (median delay: 21 months). The only risk factor for IBD development is age at AIC over 10 years. Out of 10 children genetically tested, germline variants associated with autoimmune disorders were identified in three (CTLA4: two, DOCK11: one). In children and adolescents monitored for AIC or past history of AIC, especially children over 10 years, gastro‐intestinal (GI) symptoms (recurrent abdominal pains, GI bleeding, chronic diarrhoea, weight loss) should suggest IBD and deserve specific work‐up and genetic studies. Identification of a causal germline variant will allow targeted therapy.
Forty years of human G‐CSF: A short history in time
Authors: Welte Karl 
Published: August 2024

SummaryHuman G‐CSF was identified in 1984 at Memorial Sloan‐Kettering Cancer Centre, New York. Based on these findings, recombinant G‐CSF was developed by Amgen, Thousand Oaks. In 1987, clinical trials began using recombinant G‐CSF in cancer patients following chemotherapy to reduce the duration of neutropenia and in patients with congenital neutropenia (CN) to increase the number of neutrophils. It has changed the quality of life for many cancer patients and saved the lives of many patients with (CN).
Reduction of TRPV1 expression on neurons due to downregulation of P2X7R in neonatal rat dorsal root ganglion satellite glial cells under co‐culture conditions
Authors: Wang Hongji , Chen Lisha , Xing Juping , Shi Xiangchao , Xu Changshui 
Published: August 2024

AbstractBackground information: The purinergic ligand‐gated ion channel 7 receptor (P2X7R) is an ATP‐gated ion channel that transmits extracellular signals and induces corresponding biological effects, transient receptor potential vanilloid type 1 (TRPV1) is a non‐selective cation channel that maintains normal physiological functions; numerous studies showed that P2X7R and TRPV1 are associated with inflammatory reactions. Results: The effect of P2X7R knockdown in satellite glial cells (SGCs) on neuronal TRPV1 expression under high glucose and high free fat (HGHF) environment was investigated. P2X7 short hairpin RNA (shRNA) was utilized to downregulate P2X7R in SGCs, and treated and untreated SGCs were co‐cultured with neuronal cell lines. The expression levels of inflammatory factors and signaling pathways in SGCs and neurons were measured using Western blot analysis, RT‐qPCR, immunofluorescence, and enzyme‐linked immunosorbent assays. Results suggested that P2X7 shRNA reduced the expression levels of P2X7R protein and mRNA in SGCs surrounding DRG neurons and downregulated the release of tumor necrosis factor‐alpha and interleukin‐1 beta via the Ca2+/p38 MAPK/NF‐κB pathway. Additionally, the downregulation of P2X7R might decrease TRPV1 expression in neurons via the Ca2+/PKC‐ɛ/p38 MAPK pathway.Conclusions: Reducing P2X7R expression in SCGs in an HGHF environment could decrease neuronal TRPV1 expression via the Ca2+/PKC‐ɛ/p38 MAPK pathway.
Biomarkers to inform the management of polycystic ovary syndrome: A review of systematic reviews
Authors: Walford Hugo , Tyler Bede , Abbara Ali , Clarke Sophie , Talaulikar Vikram , Wattar Bassel Al 
Published: August 2024

AbstractIntroductionPolycystic ovarian syndrome (PCOS) is the commonest endocrine condition affecting reproductive age women. Many biomarkers may aid assessment and management, however evidence is limited on their utility in clinical practice. We conducted a review of systematic reviews to identify the most useful biomarkers in the clinical management of PCOS.MethodsWe searched MEDLINE, EMBASE, CENTRAL and HTA until August 2023 for reviews evaluating biomarkers in PCOS women compared to healthy controls. Methodological quality was assessed using the AMSTAR2 tool. We reported pooled evidence for each biomarker with 95% confidence intervals from the most recent, up‐to‐date, and best quality review.ResultsFrom 3360 citations, we included 75 systematic reviews (88 biomarkers, 191,792 women). Most reviews (50/75, 67%) were moderate quality, but reported high heterogeneity (66/75, 88%). We identified 63 abnormal biomarkers in women with PCOS versus healthy controls. Of these, 22 core biomarkers could help evaluate the multisystemic impact of PCOS and inform patient management and surveillance: dehydroepiandrosterone, prolactin, sex hormone‐binding globulin, total and free testosterone, anti‐Mullerian hormone, systolic and diastolic blood pressure, c‐reactive protein, fibrinogen, oral glucose tolerance test, homoeostatic model assessment‐insulin resistance index, fasting insulin, total cholesterol, triglycerides, lipoprotein(a), HDL, LDL, non‐HDL‐cholesterol, ferritin, iron, and 25‐hydroxy‐vitamin D.ConclusionWe identified 22 core biomarkers assessing the multisystemic impact of PCOS and inform its clinical management. Future research is required to establish validated healthcare pathways.
Impact of Warm Ischemia Time on Donation After Circulatory Death Kidney Transplant Outcomes
Authors: Alghannam Karima , Fine Jeffrey , Howard Brian , Loza Jennifer , Goussous Naeem M. , Sageshima Junichiro , Mineyev Neal M. , Wang Aileen X. , Perez Richard V. , Than Peter A. 
Published: August 2024

ABSTRACTBackgroundEfforts to address the shortage of donor organs include increasing the use of renal allografts from donors after circulatory death (DCD). While warm ischemia time (WIT) is thought to be an important factor in DCD kidney evaluation, few studies have compared the relationship between WIT and DCD kidney outcomes, and WIT acceptance practices remain variable.MethodsWe conducted a single‐center retrospective review of all adult patients who underwent deceased donor kidney transplantation from 2000 to 2021. We evaluated the impact of varied functional warm ischemia time (fWIT) in controlled DCD donors by comparing donor and recipient characteristics and posttransplant outcomes between high fWIT (>60 min), low fWIT (≤60 min), and kidneys transplanted from donors after brain death (DBD).ResultsTwo thousand eight hundred eleven patients were identified, 638 received low fWIT DCD, 93 received high fWIT DCD, and 2080 received DBD kidneys. There was no significant difference in 5‐year graft survival between the DCD low fWIT, high fWIT, and DBD groups, with 84%, 83%, and 83% of grafts functioning, respectively. Five‐year patient survival was 91% in the low fWIT group, 92% in the high fWIT group, and 90% in the DBD group. An increase in kidney donor risk index (KDRI) (HR 3.37, 95% CI = 2.1–5.7) and high CIT compared to low CIT (HR 2.12, 95% CI = 1.4–3.1) have higher hazard ratios for 1‐year graft failure.ConclusionsIncreased acceptance of kidneys from selected DCD donors with prolonged fWIT may present an opportunity to increase kidney utilization while preserving outcomes. Our group specifically prioritizes the use of kidneys from younger donors, with lower KDPI, and without acute kidney injury, or risk factors for underlying chronic kidney disease.
Association of glycaemic control with intraocular pressure in a large general population: Results from the UK Biobank
Authors: Liu Qiaoling , Celis‐Morales Carlos , Sattar Naveed , Welsh Paul 
Published: August 2024

AbstractAimTo evaluate the association of glycated haemoglobin (HbA1c) and serum glucose with intraocular pressure (IOP) in a large UK general population.Materials and MethodsParticipants were selected from the UK Biobank, excluding those with eye conditions that may affect IOP. IOP was measured using an ocular response analyser. Goldmann‐correlated IOP (IOPg) and corneal‐compensated IOP (IOPcc) were outcomes of interest, and ocular hypertension was defined as left‐eye IOPg or IOPcc > 21 mmHg. HbA1c and random (non‐fasting) serum glucose were the exposures of interest. Multivariate restricted cubic spline models, as well as linear regression, were applied to explore the associations of interest.ResultsAmong 68 806 participants (46.5% male), the mean age was 56.7 years. The mean (standard deviation) for IOPg was 15.7 (3.6) mmHg and 15.9 (3.6) mmHg for IOPcc. Occular hypertension was prevalent in 8055 participants (11.7%) and 4178 participants (6.1%) had diabetes. Those with diabetes had higher IOP and a higher prevalence of ocular hypertension. After adjustment for demographic and clinical variables, HbA1c was positively associated with IOP in participants with diabetes, but not in those without diabetes. For every 10‐mmol/mol increase in HbA1c, IOPg increased by 0.20 mmHg (95% confidence interval [CI] 0.12, 0.28) and IOPcc by 0.15 mmHg (95% CI 0.07, 0.23); the odds of ocular hypertension was increased by 6% (95% CI 1.00, 1.13) in participants with diabetes. A borderline positive association between serum glucose and IOP was found only in participants without diabetes.ConclusionsImpaired glycaemic control was associated with elevated IOP and a possible risk of ocular hypertension among participants with diabetes but of normal ocular health.