bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between migraine and primary open-angle glaucoma: A two-sample Mendelian randomization study
Authors: Chaar Dima L , Yakubova Aliya , Jiang Chen , Hoffmann Thomas J , Pressman Alice , Plotnikov Denis , Choquet Hélène 
Published: February 2025

Introduction To investigate the association of genetically predicted migraine with the risk of primary open-angle glaucoma (POAG). Methods To estimate the shared genetic basis of migraine and POAG, we conducted genome-wide genetic correlation analyses using cross-trait linkage disequilibrium score regression. To assess potential causal effects of migraine on POAG risk, we conducted two-sample Mendelian randomization (MR) analyses using data from European ancestry individuals. Genetic instruments (N = 54 genetic variants with minor allele frequency (MAF) ≥ 0.01) were derived from our previous genome-wide association study (GWAS) of migraine conducted in the UK Biobank (16,709 migraine cases and 438,178 controls). For POAG, we used GWAS summary statistics from our previous GWAS conducted in the GERA cohort (3836 POAG cases and 48,065 controls) or using data from the International Glaucoma Genetics Consortium (15,229 POAG cases and 177,473 controls). The inverse-variance weighted (IVW) method was our primary source of MR estimates and common sensitivity analyses were conducted. Results We found that migraine was not genetically associated with POAG ( rg = 0.05; SE = 0.11; P = 0.69). Further, we did not detect any evidence of association between genetically predicted migraine with POAG. Conclusion Our results provide evidence that genetic risk for migraine may not be a strong causal risk factor for POAG.
Comprehensive genetic variant analysis reveals combination of KRAS and LRP1B as a predictive biomarker of response to immunotherapy in patients with non-small cell lung cancer
Authors: Eklund Ella A. , Svensson Johanna , Näslund Louise Stauber , Yhr Maria , Sayin Sama I. , Wiel Clotilde , Akyürek Levent M. , Torstensson Per , Sayin Volkan I. , Hallqvist Andreas , Raghavan Sukanya , Rohlin Anna 
Published: February 2025

Abstract

Background
In non-small cell lung cancer (NSCLC), the rapid advancement of predictive genetic testing of tumors by identifying specific pathogenic driver variants has significantly improved treatment guidance. However, immune checkpoint blockade (ICB) is typically administered to patients with tumors in the absence of such driver variants. Since only about 30% of patients will respond to ICB treatment, identifying novel genetic biomarkers of clinical response is crucial and will improve treatment decisions. This prospective clinical study aims to combine molecular biology, advanced bioinformatics and clinical data on response to treatment with ICB from a prospective cohort of NSCLC patients to identify single or combination of genetic variants in the tumor that can serve as predictive biomarkers of clinical response.

Methods
In this prospective bi-center clinical study, we performed next-generation sequencing (NGS) of 597 cancer-associated genes in a prospective cohort of 49 patients as the final cohort analyzed, with stage III or IV NSCLC, followed by establishment of an in-house developed bioinformatics-based molecular classification method that integrates, interprets and evaluates data from multiple databases and variant prediction tools. Overall survival (OS) and progression-free survival (PFS) were analyzed for selected candidate genes and variants identified using our novel methodology including molecular tools, databases and clinical information.

Results
Our novel molecular interpretation and classification method identified high impact variants in frequently altered genes KRAS, LRP1B, and TP53. Analysis of these genes as single predictive biomarkers in ICB-treated patients revealed that the presence of likely pathogenic variants and variants of unclear significance in LRP1B was associated with improved OS (p = 0.041). Importantly, further analysis of variant combinations in the tumor showed that co-occurrence of KRAS and LRP1B variants significantly improved OS (p = 0.003) and merged PFS (p = 0.008). Notably, the triple combination of variants in KRAS, LRP1B, and TP53 positively impacted both OS (p = 0.026) and merged PFS (p = 0.003).

Conclusions
This study suggests that combination of the LRP1B and KRAS variants identified through our novel molecular classification scheme leads to better outcomes following ICB treatment in NSCLC. The addition of TP53 improves the outcome even further. To our knowledge, this is the first report indicating that harboring a combination of KRAS, LRP1B, and TP53 variants can significantly enhance the response to ICB, suggesting a novel predictive biomarker combination for NSCLC patients.
High familial risks in some rare cancers may pinpoint to hidden germline genetics: focus on esophageal, stomach, small intestinal, testis, thyroid and bone cancers
Authors: Hemminki Kari , Hemminki Otto , Koskinen Anni , Hemminki Akseli , Försti Asta 
Published: February 2025

Abstract

Background
Germline genetic susceptibilities of rare cancers of the esophagus, stomach, small intestine, testis, (nonmedullary) thyroid gland and bone with high familial risks are not well known. Here, we use familial risk data from the Swedish Family-Cancer Database which contains records of cancers in Swedish families obtained over a century. We compare familial risks for offspring diagnosed with any of these cancers when their parent had or had not that cancer. We review the global literature of the reported constitutional variants that may explain part of the familial risk.

Main body
Familial risks for esophageal and stomach cancers are about 2.0 and apart from early-onset stomach cancer few high-risk variants are known. Genetic studies may be hampered by dominant environmental risk factors for these cancers. Small intestinal carcinoids have a very high familial risk (28 between siblings) but no high-risk genes have been identified to explain this. Low-risk polygenic variants have been identified. Small intestinal adenocarcinoma is a manifestation in Lynch syndrome. Testicular and thyroid cancers are characterized by high familial risk (about 5) which may be explained largely by a polygenic background, although thyroid cancer is a component in a number of rare cancer syndromes. Several predisposing genes have been identified for bone cancer (familial risk 7).

Conclusions
The discussed cancers are rare and they present with a relatively high familial risk, in spite of lacking identified high-penetrant constitutional variants. It is possible that the polygenic component, already recognized for testis cancer, is stronger than previously expected. Thus polygenic models with rare high/moderate- and low-risk variants could fit the familial risk and shape the germline genetic landscape of these cancers. Polygenic background may have clinical implications.
Corticosteroid and antimicrobial therapy in macrolide-resistant pneumococcal pneumonia porcine model
Authors: Motos Ana , Yang Minlan , Battaglini Denise , Yang Hua , Meli Andrea , Bobi Joaquim , Cabrera Roberto , Tanzella Giacomo , Vargas Carmen Rosa , Arrieta Marta , Llonch Blanca , Rovira-Ribalta Nona , Barbeta Enric , di Giannatale Pierluigi , Nogas Stefano , Fernández-Barat Laia , Rigol Montserrat , Kiarostami Kasra , Martín-Loeches Ignacio , Vila Jordi , Martinez Daniel , Bassi Gianluigi Li , Torres Antoni 
Published: February 2025

Abstract

Background

Streptococcus pneumoniae, a primary cause of community-acquired pneumonia (CAP), is typically treated with β-lactams and macrolides or quinolones. Corticosteroids are now recommended as adjunctive therapy in severe CAP to improve outcomes. In this prospective randomized animal study, we evaluated the bactericidal efficacy of various antibiotic regimens combined with corticosteroids using a porcine pneumococcal pneumonia model.

Results
In 30 White-Landrace female pigs, pneumonia was induced by intrabronchial inoculation of macrolide-resistant S. pneumoniae 19A isolate. Animals were randomized to receive saline, ceftriaxone (CRO) with levofloxacin (LVX), CRO with azithromycin (AZM), or combinations of these with methylprednisolone (MP). The primary outcome, S. pneumoniae concentrations in lung tissue after 48 h of treatment, showed that the CRO + LVX, CRO + AZM, CRO + LVX + MP, and CRO + AZM + MP groups were equally effective in reducing bacterial load. However, complete bacterial eradication from lung tissue was achieved only in the CRO + AZM + MP group. Secondary outcomes, including bacterial burden in tracheal aspirates and bronchoalveolar lavage (BAL) samples, showed similar bactericidal activity across all treatment groups. The CRO + AZM + MP group demonstrated the most controlled inflammatory response, achieving baseline levels of inflammation, while other groups exhibited elevated inflammatory markers.

Conclusions
Despite using a macrolide-resistant S. pneumoniae isolate, the combination of CRO, AZM, and MP achieves similar or even superior results compared to other antibiotic combinations. This regimen provides both bactericidal and immunomodulatory benefits, suggesting its effectiveness in treating macrolide-resistant S. pneumoniae pneumonia.
Evaluation of a new Everolimus-coated balloon catheter in an in-vivo porcine peripheral venous model
Authors: Spiliopoulos Stavros , Reppas Lazaros , Giannikas Nikolaos , Kitrou Panagiotis , Theofanis Michail , Karpetas Michail , Moulas Anargyros N. , Paraskevopoulos Ioannis , Moula Amalia I. , Ioannis Kechagias , Karnabatidis Dimitrios 
Published: February 2025

Abstract

Background
The venous uptake following the application of Everolimus-coated balloons is under reported. We evaluated the feasibility, safety, and Everolimus (EVR) deliverability of a novel non-commercially available Everolimus-Coated Balloon (ECB) catheter in a swine healthy peripheral vein model.

Methods
In total 12 ECBs (5.0 μg/mm2) were inflated in 12 venous segments. The primary feasibility endpoint was the successful application of the ECB at the target venous sites. The primary efficacy endpoint was the successful drug uptake by the target venous tissue at 24 h and 7 days, assessed by High Performance Liquid Chromatography combined with tandem mass spectrometry. The primary safety endpoint was freedom from major adverse events.

Results
Everolimus was detected in 10 out of 12 (83.33%) tissue samples (all six tissue samples at 24 h post-intervention and in four out of six samples at 7 days). The mean weight of the examined tissue was 0.20604 ± 0.29822 g (range: 0.37475–0.02229 g). The average EVR tissue content detected at 24 h (135.67 ± 204.95 μg/g) was numerically superior, but non-statistically significant to the that detected 7 days post-procedure (96.85 ± 110.89 μg/g). The average quantity of EVR on the balloon after retrieval was 33.9% of the initial drug dose. No adverse events were recorded, and no abnormalities were noted during autopsy.

Conclusions
The newly developed ECB successfully delivered Everolimus within the healthy venous wall. No adverse events were noted at a short-term follow-up.

Relevance statement
These safety and feasibility results justify further experimental and clinical research to demonstrate the safety efficacy the specific balloon catheter.
All under Heaven; or, The Evolving World Ethos of a New Greater China
Authors: Chun Allen 
Published: February 2025

Abstract
In 2022 and 2023, Duke University Press published three major works as part of its Sinotheory series. Ban Wang's book explored the political ethos of tianxia underlying the advent of the new rise of China. Pheng Cheah and Caroline S. Hau's volume of essays presented critical views of the latter in East Asia from the context of literary postcolonial theory, while Carlos Rojas and Lisa Rofel's volume surveyed the interdisciplinary landscape of China's globalist political economy, perhaps in light of recent history. Despite the mutual thematic overlap, each work interprets “China in the world” from inherently different perspectives. While they may resonate with wider discussions of nation-state, postcoloniality, and globalization elsewhere, this review essay argues that each work has its own problems of internal compatibility, especially when cast in its specific relevance or applicability to China. Instead of a sinotheory, the review essay argues that geopolitics can integrate cultural sociology as critical ethos.
The Last Korean Animation: Wonderful Days and the Aesthetics of Global Monopoly Capitalism
Authors: Gottesman Zachary Samuel 
Published: February 2025

Abstract
Wonderful Days (2003) was supposed to be the global breakthrough of Korean animation. After decades of government neglect and labor-intensive outsourcing work, the film aspired to show the world Korea's new technical prowess in animation, combining 2-D, 3-D, and miniatures in a new “Multi-Type Layer Animation.” Funded by the Korean government and Samsung's post–Asian financial crisis venture capital fund, the film also promised a new government-corporate Korean economic model in which creative labor and free-flowing finance capital would replace heavy industries and state planning. Instead, the film was a box office disaster commercially and critically, resulting in it becoming the last thematically complex, high-budget, feature-length Korean animation. What do these results tell us about the limits of its aspirations? This essay argues that the film's failure represents South Korean animation's defeat by American and Japanese monopoly capitalism. It then analyzes contradictions in the film's aesthetics and narrative to show the incompatibility of these countries’ postindustrial, ecoutopian ideology with persistent Korean manufacturing dependency and proletarian politics. The essay finally interrogates the consequences of Korean labor for thinking about transnational animation production beyond categories of success and failure determined by the nation-state and capitalism's global hierarchy of labor.
Percepción corporal y trastorno del comportamiento alimentario en universitarios españoles
Authors: Hoyos Domingo Andrea , Álvarez Rodríguez Andrea , Pérez de Villar Carmona Guadalupe , Carracedo Añón Julia María , Marrodán Serrano Mª Dolores 
Published: February 2025

Introducción: El vínculo entre la alimentación y la percepción corporal está influenciado por factores biológicos, psicológicos y socioculturales, pudiendo desembocar en hábitos peligrosos para la salud. Esto se potencia por la presión social, especialmente dirigida a las mujeres, para mantener un cuerpo delgado. Además, la etapa de la universidad es una etapa crítica que puede propiciar un mayor riesgo para el desarrollo de los trastornos del comportamiento alimentario (TCA)
Objetivos: Evaluar la asociación entre condición nutricional, percepción de la imagen corporal y satisfacción con la misma, con el riesgo de desarrollar un TCA en una muestra de jóvenes universitarios de ambos sexos.
Material y métodos: Se ha realizado una encuesta a 468 estudiantes universitarios a través de Google Forms compuesta por un test de siluetas y un cuestionario para diagnosticar riesgo de TCA (EAT-26).
Resultados: Un 61,5 % de los hombres y un 66,8 % de las mujeres se encuentra en la categoría nutricional de normopeso. Un 11,4 % de las mujeres y un 5,6 % de los hombres presentan un alto riesgo de TCA.
Discusión: El estudio muestra diferencias en la percepción y satisfacción corporal entre sexos, así como en la probabilidad de desarrollar un TCA, estando estos resultados alienados con la mayor parte de las investigaciones y revisiones precedentes.
Conclusiones: El riesgo de TCA es más frecuente en las mujeres con sobrepeso y obesidad y en los varones con insuficiencia ponderal. La percepción de la figura no se asocia de manera significativa con el riesgo de TCA, mientras que la satisfacción resulta determinante, ya que la proporción de individuos con bajo y alto riesgo de TCA es superior entre los que desearían ser más delgados, respecto a los que quisieran ser más corpulentos.
First record of ocelot Leopardus pardalis in a pine-oak forest of the Sierra Madre Occidental, Durango, México
Authors: Duarte Muñoz Michelle Alejandra , Cruz García Francisco , Cruz Cobos Francisco 
Published: February 2025

El ocelote, Leopardus pardalis, es una de las 6 especies de felinos que se distribuyen en México. Actualmente se encuentra en peligro de extinción conforme a la NOM-059-SEMARNAT-2010. Por lo que el objetivo del presente trabajo fue reportar la presencia del ocelote en la región del ejido Chavarría Nuevo, Durango, México. Se realizó un muestreo aleatorio mediante cámaras trampa en el ejido Chavarría Nuevo, Pueblo Nuevo, Durango en bosque de pino-encino. Se colocaron 8 cámaras y se obtuvo el esfuerzo de muestreo con el número de cámaras por el número de días muestreados (1,200 días trampa). Se obtuvieron fotografías independientes con el registro de varias especies de fauna; sin embargo, el ocelote se observó solo en 4 fotografías, conforme las horas en que fueron tomadas las fotografías, además del tamaño y conformación del cuerpo se identificó como un mismo individuo; sin embargo, no fue posible apreciar el sexo. En trabajos de monitoreo de mamíferos, este sería el primer registro de ocelote para la región, un área de gran importancia en el aprovechamiento forestal a nivel nacional. La presencia de esta especie sugiere un buen estado de salud del bosque, ya que en estas áreas la presencia del felino estaría asociada principalmente a su alimentación con presas como conejos, tejones y aves.
A FUNÇÃO DO ESTILO EM DELEUZE E GUATTARI
Authors: Marques de Carvalho Márcio 
Published: February 2025

Este artigo tem por objetivo apresentar a hipótese de que a experimentação de estilo na obra conjunta de Deleuze e Guattari, notadamente em Mil Platôs e em trechos de O Anti Édipo, visa impulsionar o pensamento a dinâmicas inusuais. Pela sinergia entre conceitos, afectos e perceptos, os autores integram os códigos da razão a fluxos sensíveis. Apresentam assim uma abordagem mais integral do pensamento, não limitada à recognição e desvencilhada da imagem dogmática do pensamento. Desde um paradigma ético estético Deleuze e Guattari extrapolam enquadramentos ideológicos que restringem o pensamento ao racionalismo. Apresentam cadeias heteróclitas por sintaxes incomuns para torcer a língua e tornar sensível a dinâmica dos devires. Em um fluxo rizomático, através de entinemas, buscam abordar o indiscernível. Instaurando um modo de leitura pela intensidade, conduzem o pensamento a se aventurar por domínios alheios à razão. Mais do que usar recursos de linguagens, trata-se propriamente de promover o experimentalismo no próprio pensamento.
Enhancing nutritional value and quality of cookies through pumpkin peel and seed powder fortification
Authors: Asaduzzaman Md. , Akter Mst. Sorifa , Rahman Mahbubur 
Published: February 2025

Background
Recently, the pumpkin by-products such as peels and seeds become more and more interesting for confectionary sector, because of the nutritional and bioactive composition. The study was conducted to develop cookies and noodles using different pumpkin by-products (such as peels and seeds) powders in different concentrations and to evaluate the changes in quality characteristics. The powders were prepared from pumpkin seeds and peels.

Methodology
In this study, various concentrations of pumpkin peel powder (5%, 10%, and 20%) and seeds powder (5%, 10%, 15%, and 20%) were mixed with commercially available wheat flour to produce cookies and noodles. Furthermore, the nutritional, antioxidant, functional and sensory properties of the developed cookies and noodles were evaluated.

Result
Pumpkin peels and seeds powder and their respective products enrich significantly in protein, fiber, beta-carotene, antioxidants such as total phenolic content (TPC), 2,2- diphenyl-1-picrylhydrazyl (DPPH scavenging activity), Ferric reducing ability (FRAP Ferric reducing power assay), ash content and hunter color values. However, pumpkin seed powder had higher protein content (33.25%) than other powders but pumpkin peel powder (16.83%±1.74) had better fiber content than seeds powder. The highest amount of fiber content was found in 10% pumpkin peel cookies. The analysis of the composition of pumpkin peels and seeds powder has been done in order to further promote their functionality in bakery products based, especially, on wheat flour.

Conclusion
The best quality and overall acceptable product were obtained when samples were 10% pumpkin peel powder, 15% pumpkin seed powder, and, 10% pumpkin peel powder noodles.
Impact of the COVID-19 pandemic on women in the workplace in the Middle East and North Africa: A scoping review protocol
Authors: Ghezzawi Malak , Fahme Sasha , Al Sabbagh Reem , Naalbandian Salpy , DeJong Jocelyn ,   WOMENA Study Group
Published: February 2025

Introduction
The COVID-19 pandemic has disrupted the livelihoods of working men and women worldwide. The pandemic exacerbated already existing inequities, especially in sectors where women predominate, such as the healthcare, education, and hospitality sectors. Women in the Middle East and North Africa (MENA) region, a world outlier for low female labor force participation despite high female education levels, may have been disproportionately impacted by the pandemic. Understanding the impact of COVID-19 on women’s livelihoods and health is critical to support and retain women in the labor force during external health shocks. However, until now there has been relatively little research on this topic in MENA. Therefore, this scoping review aims to determine the impact of the COVID-19 pandemic on the health and wellbeing of women workers in low-and-middle-income countries in the MENA region.

Methods
The outcome of interest is COVID-19 related occupational health (COVID-19 infection related to workplace exposures and pandemic-related occupational stressors) and the impact on mental and physical health among women workers in LMIC in MENA. Academic databases, including APA PsycINFO, Arab World Research Source: Al-Masdar, Global Health, MEDLINE, Scopus and Web of Science Core Collection will be searched. The study selection process will involve two independent reviewers and data extraction will involve summarizing key information from the included studies using a predefined charting table. The evidence will be analyzed descriptively, providing a comprehensive overview of the identified themes and patterns.

Discussion
It is anticipated that this review will facilitate a deeper understanding of the impact of the COVID-19 pandemic on working women in the MENA region. The findings may inform data-driven policies and targeted interventions that not only attract and retain women in the workforce but also enhance their health and well-being.