bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility of Cancer Immunotherapy with WT1 Peptide Vaccination for Solid and Hematological Malignancies in Children
Authors: Sawada Akihisa , Inoue Masami , Kondo Osamu , Yamada‐Nakata Kayo , Ishihara Takashi , Kuwae Yuko , Nishikawa Masanori , Ammori Yasuhiro , Tsuboi Akihiro , Oji Yusuke , Koyama‐Sato Maho , Oka Yoshihiro , Yasui Masahiro , Sugiyama Haruo , Kawa Keisei 
Published: October 2015

BackgroundAdvances in cancer immunotherapy in the pediatric field are needed in order to improve the prognosis of children with malignancies. We conducted a prospective phase I/II study of WT1 peptide vaccination for children with relapsed or refractory malignancies.MethodsThe main eligibility criteria were affected tissues or leukemic cells expressing the WT1 gene, and patients (and donors for allogeneic hematopoietic stem cell transplantation) having HLA‐A*24:02. Vaccination using the WT1 peptide (CYTWNQMNL), which was modified for higher affinity to this HLA‐type molecule with the adjuvant Montanide ISA51, was performed weekly 12 times.ResultsTwenty‐six patients were enrolled and 13 (50.0%) completed the vaccination 12 times. Evidence for the induction of WT1‐specific cytotoxic T‐lymphocyte (CTL) responses without severe systemic side effects was obtained. Two out of 12 patients with bulky disease exhibited a transient clinical effect (one mixed response and one stable disease), three out of six patients with minimal residual disease achieved transient molecular remission, and five out of eight patients without a detectable level of the molecular marker, but with a high risk of relapse, had the best outcome of long‐term continuous complete remission.ConclusionsWT1 vaccination is a safe immunotherapy and induced WT1‐specific CTL responses in children; however, as a single agent, vaccination only provided patients in remission, but with a high risk of relapse, with “long‐term benefits” in the context of its use for relapse prevention. WT1 peptide‐based treatments in combination with other modalities, such as anti‐tumor drugs or immunomodulating agents, need to be planned.
Non‐isothermal crystallization behavior of PLA/acetylated cellulose nanocrystal/silica nanocomposites
Authors: Myoung Seok Han , Im Seung Soon , Kim Seong Hun 
Published: October 2015

AbstractPoly(lactide) (PLA)/acetylated cellulose nanocrystals (ACN)/silica nanocomposites were prepared by solution casting. Surface modification of cellulose nanocrystal (CNC) was performed to prepare the ACN. The ACN and silica were expected to act as a mechanical reinforcement of PLA and a nucleation agent, respectively, to increase the crystallization rate. Introduction of acetyl groups on the surface of the cellulose nanocrystals was confirmed by Fourier transform infrared spectroscopy. A combined Avrami − Ozawa analysis described the non‐isothermal crystallization effectively. The activation energy for the crystallization was calculated from the Kissinger and the Takhor equations. Spherulite growth behavior was observed by polarizing optical microscope and spherulite growth rate, the number of spherulite versus crystallization time have investigated. The development of PLA crystals and the thermal stability had a tendency to improve with increasing silica content. Increased tensile strength was observed due to the reinforcement effect of ACN and the morphology of the nanocomposites was investigated. © 2015 Society of Chemical Industry
Effect of base‐deposited graphene oxide on the thermal stabilization of poly(vinyl chloride)
Authors: Hao Yong‐Bo , Hou Pang , Li Guo‐Xing , Li Ping , Qiu Feng , He Hong , Zeng Jian‐Bing , Wang Ming 
Published: October 2015

AbstractGraphene oxide was deposited in a base solution to form base‐deposited graphene oxide (bd‐GO) particles. The structure and properties of the bd‐GOparticles were evaluated using transmission electron microscopy, powder X‐ray diffraction and X‐ray photoelectron, Fourier transform infrared,UV‐visible and fluorescence spectroscopies. The effect of the bd‐GOparticles on the thermal stabilization of poly(vinyl chloride) (PVC) was investigated using the Congo red test and thermogravimetric analysis. The results showed that the thermal stability ofPVCwas greatly improved by the bd‐GOparticles. Furthermore, this stabilization mechanism was investigated usingUV‐visible spectroscopy and nitrogen adsorption–desorption isotherm analysis. It was found that the improvement of thermal stability was mainly related to the deactivation of thermally labile structural defects in thePVCchains by the carboxylate and alkoxide moieties of the basic groups in the bd‐GOparticles, and the highly efficient adsorption of the bd‐GOparticles with hydrogen chloride produced duringPVCdegradation. © 2015 Society of Chemical Industry
An in situ GISAXS study of selective solvent vapor annealing in thin block copolymer films: Symmetry breaking of in‐plane sphere order upon deswelling
Authors: Gunkel Ilja , Gu Xiaodan , Sun Zhiwei , Schaible Eric , Hexemer Alexander , Russell Thomas P. 
Published: October 2015

ABSTRACTThin films of asymmetric poly(styrene‐b‐4‐vinylpyridine) (PS‐b‐P4VP) block copolymers are studied by means of in situ grazing‐incidence small‐angle X‐ray scattering (GISAXS) during solvent vapor annealing in tetrahydrofuran, a solvent selective for the PS majority block of the copolymer. Upon swelling, PS‐b‐P4VP block copolymers form hexagonal arrays of spherical P4VP microdomains in a PS matrix in films 7–9 layers thick. Deswelling the films induces a transition from hexagonal to face‐centered orthorhombic (fco) symmetry, which is stable only at ∼7 layers of spherical microdomains. Dry films show co‐existing hexagonal and orthorhombic symmetries when the solvent is removed slowly, whereas instantaneous solvent removal suppresses the fco structure, resulting in films with only hexagonal structure. The in‐plane order of microdomains is significantly deteriorated in dry films independent of the solvent removal rate.Spherical block copolymer microdomains are known to undergo a transition from hexagonal to orthorhombic packing in isothermally annealed thin films when the number of sphere layers is increased from 4 to 5. In this paper, in situ GISAXS experiments reveal that a similar transition occurs during solvent vapor annealing in a selective solvent. Interestingly, the transition from hexagonal to orthorhombic packing of spheres occurs as solvent is removed from a thin block copolymer film. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2016, 54, 331–338
Exploring interaction mechanisms of the inhibitor binding to the VP35 IID region of Ebola virus by all atom molecular dynamics simulation method
Authors: Zhang Yan‐Jun , Ding Jing‐Na , Feng Ting‐Ting , Han Ju‐Guang 
Published: October 2015

AbstractEbola viruses (EBOVs) cause an acute and serious illness which is often fatal if untreated, and there is no effective vaccine until now. Multifunctional VP35 is critical for viral replication, RNA silencing suppression and nucleocapsid formation, and it is considered as a future target for the molecular biology technique. In the present work, the binding of inhibitor pyrrole‐based compounds (GA017) to wild‐type (WT), single (K248A, K251A, and I295A), and double (K248A/I295A) mutant VP35 were investigated by all‐atom molecular dynamic (MD) simulations and Molecular Mechanics Generalized Born surface area (MM/GBSA) energy calculation. The calculated results indicate that the binding with GA017 makes the binding pocket more stable and reduces the space of the binding pocket. Moreover, the electrostatic interactions (ΔEele) and VDW energy (ΔEvdw) provide the major forces for affinity binding, and single mutation I295A and double mutation K248A/I295A have great influence on the conformation of the VP35 binding pocket. Interestingly, the residues R300‐G301‐D302 of I295A form a new helix and the sheet formed by the residues V294‐I295‐H296‐I297 disappears in the double mutation K248A/I295A as compared with WT. Moreover, the binding free energy calculations show that I295A and K248A/I295A mutations decrease of absolute binding free energies while K248A and K251A mutations increase absolute binding free energy. Our calculated results are in good agreement with the experimental results that K248A/I295A double mutant results in near‐complete loss of compound binding. The obtained information will be useful for design effective inhibitors for treating Ebola virus. Proteins 2015; 83:2263–2278. © 2015 Wiley Periodicals, Inc.
A novel privacy‐preserving scheme for collaborative frequent itemset mining across vertically partitioned data
Authors: Nanavati Nirali R. , Jinwala Devesh C. 
Published: October 2015

AbstractPrivacy preservation while undertaking collaborative data mining is a significant research problem. The vertically partitioned data model is an important data partition model and has varied applications. The vertically partitioned data model necessitates a non‐collusive scheme and an efficient scheme for the problem of privacy‐preserving distributed frequent itemset mining (PPDFIM). The current literature has schemes based on secure sum, set intersection cardinality and secure binary dot product (SBDP) for PPDFIM across vertically partitioned data. [m,m] Shamir's additive secret sharing has been proposed as a non‐collusive scheme for PPDFIM in a vertically partitioned setup that uses the secure sum sub‐protocol. However, such a scheme leads to information leakage in the distributed frequent itemset mining scenario and defeats the purpose of privacy preservation. We give a critique on the non‐collusive secret sharing‐based approaches when used for privacy preservation in frequent itemset mining in a vertically partitioned model. Further, we propose Du‐Atallah's efficient multiplication protocol for SBDP of two vectors for PPDFIM. We also propose an extension of the non‐collusive Du‐Atallah's SBDP protocol for a vertically partitioned setup to mine frequent itemsets for a multi‐party multi‐vector scenario. We show how such a collusion‐resistant scheme does not lead to loss of privacy and give the theoretical and empirical analysis therein. Further, we show that our proposed scheme is more efficient than the seminal public key‐based scheme proposed by Vaidyaet al.in terms of the execution cost for a multi‐party scenario. Copyright © 2015 John Wiley & Sons, Ltd.
Family Support Needs Following Acquired Brain Injury Across Metropolitan and Regional/Remote South Australia
Authors: Bellon Michelle , Crocker Ruth , Farnden Jennifer , Gardner Jaime , Sando Sandi , Peterson Chris 
Published: October 2015

Aims:To identify and compare, family support needs following an acquired brain injury (ABI) in metropolitan and regional/remote areas in order to inform the development of a state-wide family peer support network.Design:Mixed methods design including postal survey and focus groups.Results:The survey was completed by 194 family members who provide support to an adult with ABI. Focus groups included 43 participants (29 family members, 14 people with ABI). Thematic analysis of open-ended survey responses and focus group transcripts revealed 15 areas of needed support. Although all themes were identified by both geographic groups, regional/remote participants commented more frequently on the need for coordinated, accessible and tailored services. A strong focus was placed on the need for counselling and emotional support, as well as family support groups from both major city and regional/remote participants. Each support was reviewed to identify those which could be augmented through peer-supports, including: emotional support; family support groups; ABI information; family social activities; help to navigate the system; early supports (within the first year of ABI); and self-advocacy training.Conclusions:Results highlight a need for ongoing supports for the entire family following ABI in both metropolitan and regional/remote regions of SA (South Australia). Support themes can inform the development of family-centred services, including the role of peer-support networks.
Frequency–wavenumber mapping in turbulent shear flows
Authors: de Kat Roeland , Ganapathisubramani Bharathram 
Published: October 2015

Spatial turbulence spectra for high-Reynolds-number shear flows are usually obtained by mapping experimental frequency spectra into wavenumber space using Taylor’s hypothesis, but this is known to be less than ideal. In this paper, we propose a cross-spectral approach that allows us to determine the entire wavenumber–frequency spectrum using two-point measurements. The method uses cross-spectral phase differences between two points – equivalent to wave velocities – to reconstruct the wavenumber–frequency plane, which can then be integrated to obtain the spatial spectrum. We verify the technique on a particle image velocimetry (PIV) data set of a turbulent boundary layer. To show the potential influence of the different mappings, the transfer functions that we obtained from our PIV data are applied to hot-wire data at approximately the same Reynolds number. Comparison of the newly proposed technique with the classic approach based on Taylor’s hypothesis shows that – as expected – Taylor’s hypothesis holds for larger wavenumbers (small spatial scales), but there are significant differences for smaller wavenumbers (large spatial scales). In the range of Reynolds number examined in this study, double-peaked spectra in the outer region of a turbulent wall flow are thought to be the result of using Taylor’s hypothesis. This is consistent with previous studies that focused on examining the limitations of Taylor’s hypothesis (del Álamo & Jiménez, J. Fluid Mech., vol. 640, 2009, pp. 5–26). The newly proposed mapping method provides a data-driven approach to map frequency spectra into wavenumber spectra from two-point measurements and will allow the experimental exploration of spatial spectra in high-Reynolds-number turbulent shear flows.
Combined effect ofLactobacillus acidophilusandβ-cyclodextrin on serum cholesterol in pigs
Authors: Alonso L. , Fontecha J. , Cuesta P. 
Published: October 2015

AbstractA total of twenty-four Yorkshire gilt pigs of 6–7 weeks of age were used in a 2×2 factorial experiment to determine the individual and combined effects of the inclusion of two dietary factors (cholesterol rich, 3 %β-cyclodextrin (BCD) andLactobacillus acidophiluscultures) on total cholesterol and LDL-cholesterol levels in blood serum. Pigs were assigned randomly to treatment groups (n6). Total serum cholesterol concentrations decreased after 3 weeks in all the experimental treatment groups, including diets with BCD,L.acidophilusor both. Similar trends were observed for serum LDL-cholesterol concentrations among the experimental treatments. No statistically significant differences from the control group were observed in either total serum cholesterol or LDL-cholesterol concentrations (P<0·05) for each of the individual treatment groups: BCD orL.acidophilus. However, significant differences in total serum cholesterol concentrations were observed when comparing the combined treatment group (BCD andL.acidophilus) with the control group, which consisted of a basal diet and sterile milk. The combined treatment group exhibited 17·9 % lower total serum cholesterol concentration after 3 weeks. Similar significant differences were observed when comparing the combined effect experimental group with the control group after 3 weeks. The combined treatment group exhibited 27·9 % lower serum LDL-cholesterol concentrations.
Long-term effect of residue return and fertilization on microbial biomass and community composition of a clay loam soil
Authors: ZHANG B. , GAO Q. , XU S. , MA L. , TIAN C. 
Published: October 2015

SUMMARYA field study was carried out to examine the response of microbial communities of a clay loam soil to long-term (30 years) effects of residue return and fertilization. The experimental design was a split-plot arrangement of treatments, consisting of three residue treatments (crop residues returned at rates of 0, 2500 and 5000 kg/ha) in combination with eight fertilization treatments (control, no fertilizer; N, mineral nitrogen (N) fertilizer; P, mineral phosphorus (P) fertilizer; K, mineral potassium (K) fertilizer; NP, mineral NP fertilizer; NK, mineral NK fertilizer; PK, mineral PK fertilizer; and NPK, mineral NPK fertilizer). Soil microbial communities were characterized by phospholipid fatty acid analysis. Results indicated that the more crop residues were returned, the lower ratio of fungi to bacteria was observed. However, soil microbial biomass was only found to be significantly higher in plots with residues returned at a rate of 5000 kg/ha but not 2500 kg/ha. This suggested there was a threshold for microbial biomass to increase under residue return for the clay loam soil studied. The fertilization effect on soil microbial biomass gradually decreased with increases in the amount of crop residues returned. A significant composition change was observed under N fertilization. Structural equation modelling indicated that soil microbial communities were influenced directly by residue return and indirectly by residue-induced change in ratio of carbon to N and fertilization-induced change in soil pH.
The radical humility of Christ in the sixteenth century: Erasmus and Calvin on Philippians 2:6–7
Authors: Essary Kirk 
Published: October 2015

AbstractThe christological hymn in Philippians 2, rich as it is in theological potential, has always been a fruitful locus in the history of biblical interpretation for engaging in a number of doctrinal disputes which revolve around questions of the nature of Christ. Thus, an analysis of any chapter in the history of interpretation of the hymn (or at least parts of it) is necessary for understanding the ways in which Paul's text has informed christological discourse or, vice versa, how certain ways of thinking about Christology inform interpretations of the passage. In the sixteenth century, the hymn also serves as a jumping-off point for discussions of the authority of scripture in matters of doctrine, for whether Paul provides sufficient doctrinal fodder to ground an orthodox doctrine of the Trinity (particularly of the consubstantiality of the Father and the Son) will be brought into question, in particular, by Erasmus. Erasmus’ understanding of the passage, as it appears in hisAnnotations, was criticised by numerous Catholics, and the ensuing debate (especially between Erasmus and Lefèvre) is fairly well known. The response Erasmus (and the surrounding debate) elicits from John Calvin, however, has scarcely been mentioned and, to my knowledge, never been examined in depth – this, despite the fact that Calvin's engagement with Erasmus on Philippians 2:6–7 departs from his usual method ofperspicua brevitasin commentary writing, and constitutes a significant digression on an array of christological and hermeneutical issues. These two verses, and their reception in the sixteenth century, provide a useful lens for analysing the christologies and the hermeneutical strategies of two biblical humanists who, perhaps, are not often enough considered alongside one another. A close reading of these two exegetes’ interpretations of Philippians 2:6–7 will be followed by a consideration of the significance of their emphasis on the radical humility of Christ, which emphasis serves as a departure from the bulk of the antecedent exegetical and theological tradition.
‘“Fuisse in Forma Hominis” belongs to Christ Alone’: John Calvin's trinitarian hermeneutics in hisLectures on Ezekiel
Authors: Slotemaker John T. 
Published: October 2015

AbstractThe present article examines John Calvin's trinitarian and christological interpretation of Old Testament theophanies in hisPraelectioneson Ezekiel 1. The first section of the article treats Calvin's exegetical principles. It is noted that Calvin defends a strict set of rules for how to interpret Old Testament theophanies: in short, Calvin argues that if a passage presents the divine nature in the form of a human person, that given theophany must be interpreted as a representation of the second person of the Trinity, the Son of God (i.e. Jesus Christ). In defending this position, Calvin examines in great detail various rules for how to interpret Old Testament passages which indicate a plurality within the divine nature (i.e. the Trinity). He defends his exegetical approach to these texts with numerous passages from the New Testament.This examination of Calvin's exegesis is contextualised in two ways. First, it is noted that Calvin's exegesis of these passages is uncharacteristically more ‘strict’ in its trinitarian and christological reading than one finds in earlier thinkers such as Augustine and Jerome. For example, Augustine argued that Old Testament theophanies which present God in the form of a human being could be understood as the Father, the Son or the Holy Spirit. Augustine, in short, does not think one can definitively determine which member of the Trinity is ‘present’ in a theophany. Second, it is noted that this surprising development in Calvin's final work is the result of the rising threat of anti-trinitarianism in Transylvania. Thus, the article argues that the rise of Polish anti-trinitarianism not only contributed to Calvin's renewed interest in trinitarian and christological interpretations of the Old Testament, but it also pushed him to develop a more strict set of exegetical rules which govern how such passages are interpreted.Therefore, the article presents a reading of Calvin which strongly suggests that any complete analysis of Calvin's alleged ‘Judaising’ must develop a historically nuanced methodology. While it is often argued that Calvin hesitates from interpreting Old Testament passages in a strictly trinitarian or christological way, it must be acknowledged that towards the end of his career he radically began to alter his exegetical rules/method given the renewed threat of the anti-trinitarians.