bluuebunny/crossref_metadata_2025_split huggingface dataset:
Capping protein beta is required for actin cytoskeleton organisation and cell migration during Drosophila oogenesis
Authors: Ogienko Anna A. , Karagodin Dmitry A. , Lashina Valentina V. , Baiborodin Sergey I. , Omelina Eugeniya S. , Baricheva Elina M. 
Published: August 2013

AbstractCapping protein (CP) is a well‐characterised actin‐binding protein important for regulation of actin filament (AF) assembly. CP caps the barbed end of AFs, inhibiting the addition and loss of actin monomers. In Drosophila melanogaster, the gene encoding CP β‐subunit is named capping protein beta (cpb; see Hopmann et al. [1996] J Cell Biol 133: 1293–305). The cpb level is reduced in the Drosophila bristle actin cytoskeleton and becomes disorganised with abnormal morphology. A reduced level of the CP protein in ovary results in disruption of oocyte determination, and disturbance of nurse cell (NC) cortical integrity and dumping. We describe novel defects appearing in cpb mutants during oogenesis, in which cpb plays an important role in border and centripetal follicle cell migration, ring canal development and cytoplasmic AF formation. The number of long cytoplasmic AFs was dramatically reduced in cpb hypomorphs and abnormal actin aggregates was seen on the inner side of NC membranes. A hypothesis to explain the formation of abnormal short‐cut cytoplasmic AFs and actin aggregates in the cpb mutant NCs was proffered, along with a discussion of the reasons for ‘dumpless’ phenotype formation in the mutants.
Upregulation of HO‐1 is accompanied by activation of p38MAPK and mTOR in human oesophageal squamous carcinoma cells
Authors: Hu Jian‐Li , Xiao Lan , Li Zhen‐Yun , Wang Qiong , Chang Yu , Jin Yi 
Published: August 2013

AbstractInduction of HO‐1 protein can have both beneficial and detrimental effects for cells, including regulating proliferation and apoptosis of several tumours. We have investigated the regulation of HO‐1, p38MAPK and mTOR in the context of proliferation, cell cycle events and apoptosis of oesophageal squamous cell carcinoma cells. Real‐time PCR and Western blots were used to determine the expression levels of HO‐1, p38MAPK, p‐p38MAPK and p‐mTOR. MTT assays were used to measure proliferation, FACS for cell cycle events and Annexin V staining for apoptosis. Proliferation of Eca109 cells was inhibited and apoptosis was induced in the presence of p38MAPK inhibitor (SB203580) and mTOR inhibitor (Rapamycin, RAPA). HO‐1 expression was downregulated in cells treated with SB203580 and RAPA. HO‐1 overexpression inhibited apoptosis and induced G2/M arrest in SB203580 and RAPA‐treated cells. HO‐1 expression was upregulated in the presence of ethanol, and was accompanied by activation of p38MAPK and mTOR. However, ethanol‐treated cells exposed to HO‐1 inhibitor showed no effect on p38MAPK and mTOR activation. The data suggest that ethanol‐induced upregulation of HO‐1 in oesophageal squamous cell carcinoma is accompanied by the activation of the p38MAPK and mTOR pathways.
Injectable thermosensitive chitosan/β‐glycerophosphate/collagen hydrogel maintains the plasticity of skeletal muscle satellite cells and supports their in vivo viability
Authors: Ding Ke , Yang Zhong , Zhang Yu‐Long , Xu Jian‐Zhong 
Published: August 2013

AbstractA cell carrier plays an important role in the maintenance, growth and engraftment of specific cells aimed for defined therapeutic uses in many tissue engineering strategies. A suitable microenvironment for the cells allows for the maximum efficacy of the hybrid device. We have prepared an injectable thermosensitive chitosan/β‐glycerophosphate/collagen (C/GP/Co) gel and investigated its potential application as a support for the culture of skeletal muscle satellite cells (SMSCs). A cell viability assay was used to evaluate the in vitro cytocompatibility of the gel. Cell growth was assessed by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM) and histological analysis. The influence of the C/GP/Co gel on the plasticity of SMSCs seeded at the surface of the gel was assessed by induction of the myogenic, osteogenic and adipogenic differentiation. C/GP/Co gel provided the appropriate environment for the culture of SMSCs in vitro. In addition, the C/GP/Co gel supported SMSC plasticity. In vivo testing of the SMSC‐seeded gel was investigated by subcutaneous injection into the dorsum of nude mice. Cell viability was assessed both by in vivo imaging and histological examination of the explants. In conclusion, C/GP/Co hydrogel is a cytocompatible carrier for the in vivo delivery of SMSCs and supportive for SMSC plasticity. Thus, this gel has potential applications in tissue engineering and regenerative medicine.
Corilagin inhibits hepatocellular carcinoma cell proliferation by inducing G2/M phase arrest
Authors: Ming Yanlin , Zheng Zhizhong , Chen Lianghua , Zheng Guohua , Liu Shaosong , Yu Yinhua , Tong Qingxuan 
Published: August 2013

AbstractHepatocellular carcinoma (HCC) is one of most common types of malignant tumours. Therefore, it is very important to identify powerful drugs and their antitumour mechanisms. Corilagin has a significant antitumour potential and lower toxicity in normal cells in vitro. The IC50 values of corilagin for normal Chang‐liver cells and the HCC cell lines Bel7402 and SMMC7721 were 131.4, 24.5 and 23.4 µM, respectively, in the methyl thiazolyl tetrazolium (MTT) assay. MHCC97‐H xenografts in Balb/c mice intraperitoneally injected with 30 mg/kg corilagin for 5 weeks showed a 47.3% inhibition of tumour growth in vivo. Furthermore, data from flow cytometry and Western blot analyses of cell cycle and cell cycle‐related proteins suggest that corilagin arrests SMMC7721 cells at the G2/M phase by downregulating p‐Akt and cyclin B1/cdc2 and upregulating p‐p53 and p21Cip1. In conclusion, corilagin is a potential antitumour drug that is effective in retarding the growth of HCC, which is correlated with the activation of p‐p53‐p21Cip1‐cdc2/cyclin B1.
Immortality of cell lines: challenges and advantages of establishment
Authors: Irfan Maqsood Muhammad , Matin Maryam M. , Bahrami Ahmad Reza , Ghasroldasht Mohammad M. 
Published: August 2013

AbstractCellular immortality happens upon impairment of cell‐cycle checkpoint pathways (p53/p16/pRb), reactivation or up‐regulation of telomerase enzyme, or upregulation of some oncogenes or oncoproteins leading to a higher rate of cell division.There are also some other factors and mechanisms involved in immortalisation, which need to be discovered. Immortalisation of cells derived from different sources and establishment of immortal cell lines has proven useful in understanding the molecular pathways governing cell developmental cascades in eukaryotic, especially human, cells. After the breakthrough of achieving the immortal cells and understanding their critical importance in the field of molecular biology, intense efforts have been dedicated to establish cell lines useful for elucidating the functions of telomerase, developmental lineage of progenitors, self‐renewal potency, cellular transformation, differentiation patterns and some bioprocesses, like odontogenesis. Meanwhile, discovering the exact mechanisms of immortality, a major challenge for science yet, is believed to open new gateways toward understanding and treatment of cancer in the long term. This review summarises the methods involved in establishing immortality, its advantages and the challenges still being faced in this field.
TNF‐α triggers osteogenic differentiation of human dental pulp stem cells via the NF‐κB signalling pathway
Authors: Feng Xingmei , Feng Guijuan , Xing Jing , Shen Biyu , Li Liren , Tan Wei , Xu Yue , Liu Suzhe , Liu Hong , Jiang Jinxia , Wu Hao , Tao Tao , Gu Zhifeng 
Published: August 2013

AbstractDental pulp stem cells (DPSCs) are a type of mesenchymal stem cells (MSCs) characterised by self‐renewal and multi‐lineage differentiation, including chondrocytes, adipocytes, neural cells and osteoblasts, which make it an attractive choice for tissue engineering purposes. Tumour necrosis factor α (TNF‐α) had the positive effect on the mineralisation of bone marrow MSCs and stromal cells derived from human adipose tissue. However, the effect of TNF‐α on DPSCs is unclear. We found that TNF‐α activated the NF‐κB pathway during the osteogenic differentiation of DPSCs. TNF‐α also increased mineralisation and the expression of bone morphogenetic protein 2 (BMP2), alkaline phosphatase (ALP), runt‐related transcription factor 2 (RUNX2) and collagen type I (COL I) during this process. PDTC, an NF‐κB inhibitor, blocked the osteogenic differentiation induced by TNF‐α. No effect of TNF‐α on proliferation of DPSCs or cell cycle was detected. In summary, TNF‐α promotes mineralisation and mineralisation‐related gene expression through the NF‐κB signalling pathway in DPSCs, which may provide a foundation for autologous transplantation of DPSCs.
Hydrodynamics of High‐Pressure Bubble Columns
Authors: Rollbusch P. , Tuinier M. , Becker M. , Ludwig M. , Grünewald M. , Franke R. 
Published: August 2013

AbstractBubble column hydrodynamics influence important performance parameters like conversion, selectivity, and yield of a chemical reaction. Backmixing of the liquid phase and gas holdup are thereby of particular importance. In order to determine these parameters, a number of correlations exist in the literature. These correlations are mainly derived from small‐scale equipment operated under atmospheric conditions using the physical system air/water. Published experimental results regarding gas holdups and dispersion in bubble columns operated under high pressure are discussed and a recommendation for further research is given.
Parameter optimisation and validation for droplet population balances
Authors: Jildeh Hanin B. , Attarakih Menwer , Mickler Matthias , Bart Hans‐Jörg 
Published: August 2013

AbstractSingle droplet experiments in Kühni compartment for five different liquid systems were used to evaluate Schmidt breakage model parameters. Based on these optimised parameters, Coulaloglou and Tavlarides coalescence model parameters were obtained by an inverse solution of population balance model (PBM) using the extended fixed‐pivot technique. The estimated interaction parameters were used to study the hydrodynamics of a pilot Kühni column. For online prediction purposes the One Primary and One Secondary Particle Model (OPOSPM), which is essentially a reduced population balance model (PBM), was used as an alternative to the computational expensive detailed PBM. Different test systems and column geometries were used for steady and transient state validation where OPOSPM was found to produce remarkable good results.
Online monitoring, simulation and prediction of multiphase flows
Authors: Mickler Matthias , Jildeh Hanin B. , Attarakih Menwer , Bart Hans‐Jörg 
Published: August 2013

AbstractAn online monitoring and simulation tool (OMST) is used to determine, analyse, simulate and predict the multiphase flow behaviour in an extraction column within the context of model predictive control (MPC). The simulation was done with the droplet population model OPOSPM using an adapted moments method for online simulation. For validation, steady‐state and transient experiments are performed, where rotor speed and dispersed volume flow rate are changed using the EFCE system toluene–water. OMST deviations are between 3% (droplet sizes in steady state) and 20% (hold‐up in dynamic step‐changes) which are highly dependent on the quality of the parameter estimation.
Evaluating comparative effectiveness with observational data
Authors: Parmar Abhishek D. , Sheffield Kristin M. , Han Yimei , Vargas Gabriela M. , Guturu Praveen , Kuo Yong‐Fang , Goodwin James S. , Riall Taylor S. 
Published: August 2013

BACKGROUNDA previous observational study reported that endoscopic ultrasound (EUS) is associated with improved survival in older patients with pancreatic cancer. The objective of this study was to reevaluate this association using different statistical methods to control for confounding and selection bias.METHODSSurveillance, Epidemiology, and End Results (SEER)‐Medicare linked data (1992‐2007) was used to identify patients with locoregional pancreatic cancer. Two‐year survival in patients who did and did not receive EUS was compared by using standard Cox proportional hazards models, propensity score methodology, and instrumental variable analysis.RESULTSEUS was associated with improved survival in both unadjusted (hazard ratio [HR] = 0.67, 95% confidence interval [CI] = 0.63‐0.72) and standard regression analyses (HR = 0.78, 95% CI = 0.73‐0.84) which controlled for age, sex, race, marital status, tumor stage, SEER region, Charlson comorbidity, year of diagnosis, education, preoperative biliary stenting, chemotherapy, radiation, and pancreatic resection. Propensity score adjustment, matching, and stratification did not attenuate this survival benefit. In an instrumental variable analysis, the survival benefit was no longer observed (HR = 1.00, 95% CI = 0.73‐1.36).CONCLUSIONSThese results demonstrate the need to exercise caution in using administrative data to infer causal mortality benefits with diagnostic and/or treatment interventions in cancer research. Cancer 2013;119:3861–3869. © 2013 American Cancer Society.
A phase 2 trial of extended induction epratuzumab and rituximab for previously untreated follicular lymphoma: CALGB 50701
Authors: Grant Barbara W. , Jung Sin‐Ho , Johnson Jeffrey L. , Kostakoglu Lale , Hsi Eric , Byrd John C. , Jones Jeffrey , Leonard John P. , Martin S. Eric , Cheson Bruce D. 
Published: August 2013

BACKGROUNDRituximab combined with chemotherapy has improved the survival of previously untreated patients with follicular lymphoma (FL). Nevertheless, many patients neither want nor can tolerate chemotherapy, leading to interest in biological approaches. Epratuzumab is a humanized anti‐CD22 monoclonal antibody with efficacy in relapsed FL. Because both rituximab and epratuzumab have single‐agent activity in FL, the antibody combination was evaluated as initial treatment of patients with FL.METHODSFifty‐nine untreated patients with FL received epratuzumab 360 mg/m2 with rituximab 375 mg/m2 weekly for 4 induction doses. This combination was continued as extended induction in weeks 12, 20, 28, and 36. Response assessed by computed tomography was correlated with clinical risk factors, [18F]fluorodeoxyglucose positron emission tomography findings at week 3, Fcγ polymorphisms, immunohistochemical markers, and statin use.RESULTSTherapy was well‐tolerated, with toxicities similar to expected with rituximab monotherapy. Fifty‐two (88.2%) evaluable patients responded, including 25 complete responses (42.4%) and 27 partial responses (45.8%). At 3 years follow‐up, 60% of patients remain in remission. Follicular Lymphoma International Prognostic Index (FLIPI) risk strongly predicted progression‐free survival (P = .022).CONCLUSIONSThe high response rate and prolonged time to progression observed with this antibody combination are comparable to those observed after standard chemoimmunotherapies and further support the development of biologic, nonchemotherapeutic approaches for these patients. Cancer 2013;119:3797–3804. © 2013 American Cancer Society.
Associations of breastfeeding with bulimic behaviors and eating disorders among adolescents
Authors: Iron‐Segev Sharon , Peterson Karen E. , Gillman Matthew W. , Williams Corrine M. , Austin S. Bryn , Field Alison E. 
Published: August 2013

ABSTRACTPurposeTo use the lifecourse framework to examine the association between duration of breastfeeding and risk of developing bulimic behaviors or a diagnosed eating disorder.MethodQuestionnaires were sent every 12–24 months between 1996 and 2005 to 6,436 females and 5,756 males in the Growing Up Today Study, who were 9–14 years at baseline. Duration of breastfeeding was reported by the participants' mothers in 1997. We used generalized estimating equations to estimate the association of breastfeeding with purging, binge eating, engaging in bulimic behaviors, and having a diagnosed eating disorder.ResultsCompared to girls who were breastfed for more than 9 months, those who were breastfed for less than 4 months did not have a significantly different prevalence of purging, binge eating, bulimic behaviors, and self‐reported history of diagnosed eating disorders. Adjusting for gestational age/birthweight, age, age at menarche, maternal history of an eating disorder, and maternal body mass index, short duration of breastfeeding was not associated with any outcome among the girls [adjusted odds ratios (AOR) ranged from 0.8 to 1.1]. Among the boys, the results showed no significant associations between duration of breastfeeding and purging, binge eating, and self‐reported history of diagnosed eating disorder. However, there was a suggestion that boys who had been breastfed for less than 4 months were at a higher risk of engaging in bulimic behaviors [AOR: 1.5, 95% confidence interval (CI), 1.0–2.3].DiscussionNo association was found between duration of breastfeeding and risk of developing bulimic behaviors or a diagnosed eating disorder among girls or boys with the one exception of longer duration of breastfeeding associated with fewer bulimic behaviors in boys. Although there are many benefits to breastfeeding, our data suggest that breastfeeding does not offer any protection against binge eating or purging, nor does it present harmful effects. © 2013 Wiley Periodicals, Inc. (Int J Eat Disord 2013; 46:834–840)
Small‐Molecule Activation by Reactive Metal Complexes
Authors: Lu Connie C. , Meyer Karsten 
Published: August 2013

AbstractAn overview of the papers in this cluster issue is presented.One of the grand challenges is to develop clean energy for a global green economy. Chemists – as creators and manipulators of molecules – will play a tremendous role towards this future. We will be relied upon to invent and improve catalysts for many useful and needed reactions, of which one of the most important class is the conversion of small molecules of biological and industrial relevance, including H2, H2O, CH4, CO2, N2, and O2...Read more by the Guest Editors on p. 3731.
Population pharmacokinetic analysis from phase I and phase II studies of the humanized monovalent antibody, onartuzumab (MetMAb), in patients with advanced solid tumors
Authors: Xin Yan , Jin Denise , Eppler Stephen , Damico‐Beyer Lisa A. , Joshi Amita , Davis John D. , Kaur Surinder , Nijem Ihsan , Bothos John , Peterson Amy , Patel Premal , Bai Shuang 
Published: August 2013

AbstractOnartuzumab is a unique, humanized, monovalent (one‐armed) monoclonal antibody (mAb) against the MET receptor. The intravenous (IV) pharmacokinetics (PK) of onartuzumab were investigated in a phase I study and a phase II study in recurrent non‐small cell lung cancer (NSCLC) patients. The potential for drug–drug interaction (DDI) was assessed during co‐administration of IV onartuzumab with oral erlotinib, by measuring the PK of both drugs. The concentration–time profiles of onartuzumab were adequately described using a two‐compartment model with linear clearance (CL) at doses between 4 and 30 mg/kg. The estimates for CL, central compartment volume (V1), and median terminal half‐life were 0.439 L/day, 2.77 L, and 13.4 days, respectively. Statistically significant covariates included creatinine clearance (CrCL) on clearance, weight and gender on V1, and weight on peripheral compartment volume (V2), but the clinical relevance of these covariates needs to be further evaluated. The current analysis did not indicate obvious DDI between onartuzumab and erlotinib. MET diagnostic status did not impact the exposure of either agent. Despite the slightly faster clearance compared with typical bivalent mAbs, the PK of onartuzumab support dosing regimens of 15 mg/kg every 3 weeks or doses equivalent to achieve the target minimum tumoristatic concentration in patients.
Stratigraphic architecture and fault offsets of alluvial terraces at Te Marua, Wellington fault, New Zealand, revealed by pseudo‐3D GPR investigation
Authors: Beauprêtre S. , Manighetti I. , Garambois S. , Malavieille J. , Dominguez S. 
Published: August 2013

AbstractPast earthquake slips on faults are commonly determined by measuring morphological offsets at current ground surface. Because those offsets might not always be well preserved, we examine whether the first 10 m below ground surface contains relevant information to complement them. We focus on the Te Marua site, New Zealand, where 11 alluvial terraces have been dextrally offset by the Wellington fault. We investigated the site using pseudo‐3D Ground Penetrating Radar and also produced a high‐resolution digital elevation model (DEM) of the zone to constrain the surface slip record. The GPR data reveal additional information: (1) they image the 3D stratigraphic architecture of the seven youngest terraces and show that they are strath terraces carved into graywacke bedrock. Each strath surface is overlain by 3–5 m of horizontally bedded gravel sheets, including two pronounced and traceable reflectors; (2) thanks to the multilayer architecture, terrace risers and channels are imaged at three depths and their lateral offsets can be measured three to four times, constraining respective offsets and their uncertainties more reliably; and (3) the offsets are better preserved in the subsurface than at the ground surface, likely due to subsequent erosion‐deposition on the latter. From surface and subsurface data, we infer that Te Marua has recorded six cumulative offsets of 2.9, 7.6, 18, 23.2, 26, and 31 m (± 1–2 m). Large earthquakes on southern Wellington fault might produce 3–5 m of slip, slightly less than previously proposed. Pseudo‐3D GPR thus provides a novel paleoseismological tool to complement and refine surface investigations.
A review of abusive supervision research
Authors: Martinko Mark J. , Harvey Paul , Brees Jeremy R. , Mackey Jeremy 
Published: August 2013

SummaryThis paper reviews studies concerned with abusive supervision and provides a constructive revision of Tepper's 2007 model. As a result of our review of the recent research, we revised the 2007 Tepper model and added additional variables and casual paths to increase its explanatory potential. The model we propose distinguishes between abusive supervisory behavior and abusive supervisory perceptions, suggesting that each of these variables needs to be studied separately until we know more about how they are related. The revised model also explicitly recognizes possibilities for reverse causation and stresses the importance of subordinates' individual differences such as attribution style, negative affectivity, and implicit work theories, which have the potential to account for significant variability in subordinates' perceptions of abuse. Suggestions for future research based on the original relationships identified by the Tepper review as well as the variables and causal paths suggested in the revised model are provided. Copyright © 2013 John Wiley & Sons, Ltd.