bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phosphotyrosine recognition domains: the typical, the atypical and the versatile
Authors: Kaneko Tomonori , Joshi Rakesh , Feller Stephan M , Li Shawn SC 
Published: November 2012

Abstract
SH2 domains are long known prominent players in the field of phosphotyrosine recognition within signaling protein networks. However, over the years they have been joined by an increasing number of other protein domain families that can, at least with some of their members, also recognise pTyr residues in a sequence-specific context. This superfamily of pTyr recognition modules, which includes substantial fractions of the PTB domains, as well as much smaller, or even single member fractions like the HYB domain, the PKCδ and PKCθ C2 domains and RKIP, represents a fascinating, medically relevant and hence intensely studied part of the cellular signaling architecture of metazoans. Protein tyrosine phosphorylation clearly serves a plethora of functions and pTyr recognition domains are used in a similarly wide range of interaction modes, which encompass, for example, partner protein switching, tandem recognition functionalities and the interaction with catalytically active protein domains. If looked upon closely enough, virtually no pTyr recognition and regulation event is an exact mirror image of another one in the same cell. Thus, the more we learn about the biology and ultrastructural details of pTyr recognition domains, the more does it become apparent that nature cleverly combines and varies a few basic principles to generate a sheer endless number of sophisticated and highly effective recognition/regulation events that are, under normal conditions, elegantly orchestrated in time and space. This knowledge is also valuable when exploring pTyr reader domains as diagnostic tools, drug targets or therapeutic reagents to combat human diseases.
Quantum conductance of silicon-doped carbon wire nanojunctions
Authors: Szczȩśniak Dominik , Khater Antoine , Ba̧k Zygmunt , Szczȩśniak Radosław , Ghantous Michel Abou 
Published: November 2012

AbstractUnknown quantum electronic conductance across nanojunctions made of silicon-doped carbon wires between carbon leads is investigated. This is done by an appropriate generalization of the phase field matching theory for the multi-scattering processes of electronic excitations at the nanojunction and the use of the tight-binding method. Our calculations of the electronic band structures for carbon, silicon, and diatomic silicon carbide are matched with the available corresponding density functional theory results to optimize the required tight-binding parameters. Silicon and carbon atoms are treated on the same footing by characterizing each with their corresponding orbitals. Several types of nanojunctions are analyzed to sample their behavior under different atomic configurations. We calculate for each nanojunction the individual contributions to the quantum conductance for the propagatingσ,Π, andσ∗electron incidents from the carbon leads. The calculated results show a number of remarkable features, which include the influence of the ordered periodic configurations of silicon-carbon pairs and the suppression of quantum conductance due to minimum substitutional disorder and artificially organized symmetry on these nanojunctions. Our results also demonstrate that the phase field matching theory is an efficient tool to treat the quantum conductance of complex molecular nanojunctions.
Cardiac-specific catalase overexpression rescues anthrax lethal toxin-induced cardiac contractile dysfunction: role of oxidative stress and autophagy
Authors: Kandadi Machender R , Yu Xuejun , Frankel Arthur E , Ren Jun 
Published: November 2012

AbstractBackgroundLethal and edema toxins secreted byBacillus anthracisduring anthrax infection were found to incite serious cardiovascular complications. However, the underlying mechanisms in anthrax lethal toxin-induced cardiac anomalies remain unknown. This study was designed to evaluate the impact of antioxidant enzyme catalase in anthrax lethal toxin-induced cardiomyocyte contractile dysfunction.MethodsWild type (WT) and cardiac-specific catalase overexpression mice were challenged with lethal toxin (2 μg/g, intraperotineally (i.p.)). Cardiomyocyte contractile and intracellular Ca2+properties were assessed 18 h later using an IonOptix edge-detection system. Proteasome function was assessed using chymotrypsin-like and caspase-like activities. GFP-LC3 puncta and Western blot analysis were used to evaluate autophagy and protein ubiquitination.ResultsLethal toxin exposure suppressed cardiomyocyte contractile function (suppressed peak shortening, maximal velocity of shortening/re-lengthening, prolonged duration of shortening/re-lengthening, and impaired intracellular Ca2+handling), the effects of which were alleviated by catalase. In addition, lethal toxin triggered autophagy, mitochondrial and ubiquitin-proteasome defects, the effects of which were mitigated by catalase. Pretreatment of cardiomyocytes from catalase mice with the autophagy inducer rapamycin significantly attenuated or ablated catalase-offered protection against lethal toxin-induced cardiomyocyte dysfunction. On the other hand, the autophagy inhibitor 3-MA ablated or significantly attenuated lethal toxin-induced cardiomyocyte contractile anomalies.ConclusionsOur results suggest that catalase is protective against anthrax lethal toxin-induced cardiomyocyte contractile and intracellular Ca2+anomalies, possibly through regulation of autophagy and mitochondrial function.
Delineating the core regulatory elements crucial for directed cell migration by examining folic-acid-mediated responses
Authors: Srinivasan Kamalakkannan , Wright Gus A. , Hames Nicole , Housman Max , Roberts Alayna , Aufderheide Karl J. , Janetopoulos Chris 
Published: November 2012

Summary
Dictyostelium discoideum shows chemotaxis towards folic acid (FA) throughout vegetative growth, and towards cAMP during development. We determined the spatiotemporal localization of cytoskeletal and signaling molecules and investigated the FA-mediated responses in a number of signaling mutants to further our understanding of the core regulatory elements that are crucial for cell migration. Proteins enriched in the pseudopods during chemotaxis also relocalize transiently to the plasma membrane during uniform FA stimulation. In contrast, proteins that are absent from the pseudopods during migration redistribute transiently from the PM to the cytosol when cells are globally stimulated with FA. These chemotactic responses to FA were also examined in cells lacking the GTPases Ras C and G. Although Ras and phosphoinositide 3-kinase activity were significantly decreased in Ras G and Ras C/G nulls, these mutants still migrated towards FA, indicating that other pathways must support FA-mediated chemotaxis. We also examined the spatial movements of PTEN in response to uniform FA and cAMP stimulation in phospholipase C (PLC) null cells. The lack of PLC strongly influences the localization of PTEN in response to FA, but not cAMP. In addition, we compared the gradient-sensing behavior of polarized cells migrating towards cAMP to that of unpolarized cells migrating towards FA. The majority of polarized cells make U-turns when the cAMP gradient is switched from the front of the cell to the rear. Conversely, unpolarized cells immediately extend pseudopods towards the new FA source. We also observed that plasma membrane phosphatidylinositol 3,4,5-trisphosphate [PtdIns(3,4,5)P3] levels oscillate in unpolarized cells treated with Latrunculin-A, whereas polarized cells had stable plasma membrane PtdIns(3,4,5)P3 responses toward the chemoattractant gradient source. Results were similar for cells that were starved for 4 hours, with a mixture of polarized and unpolarized cells responding to cAMP. Taken together, these findings suggest that similar components control gradient sensing during FA- and cAMP-mediated motility, but the response of polarized cells is more stable, which ultimately helps maintain their directionality.
Glutathione peroxidase 3, a new retinoid target gene, is crucial for human skeletal muscle precursor cell survival
Authors: El Haddad Marina , Jean Elise , Turki Ahmed , Hugon Gérald , Vernus Barbara , Bonnieu Anne , Passerieux Emilie , Hamade Aline , Mercier Jacques , Laoudj-Chenivesse Dalila , Carnac Gilles 
Published: November 2012

SummaryProtection of satellite cells from cytotoxic damages is crucial to ensure efficient adult skeletal muscle regeneration and to improve therapeutic efficacy of cell transplantation in degenerative skeletal muscle diseases. It is therefore important to identify and characterize molecules and their target genes that control the viability of muscle stem cells. Recently, we demonstrated that high aldehyde dehydrogenase activity is associated with increased viability of human myoblasts. In addition to its detoxifying activity, aldehyde dehydrogenase can also catalyze the irreversible oxidation of vitamin A to retinoic acid; therefore, we examined whether retinoic acid is important for myoblast viability. We showed that when exposed to oxidative stress induced by hydrogen peroxide, adherent human myoblasts entered apoptosis and lost their capacity for adhesion. Pre-treatment with retinoic acid reduced the cytotoxic damage ex vivo and enhanced myoblast survival in transplantation assays. The effects of retinoic acid were maintained in dystrophic myoblasts derived from facioscapulohumeral patients. RT-qPCR analysis of antioxidant gene expression revealed glutathione peroxidase 3 (Gpx3), a gene encoding an antioxidant enzyme, as a potential retinoic acid target gene in human myoblasts. Knockdown of Gpx3 using short interfering RNA induced elevation in reactive oxygen species and cell death. The anti-cytotoxic effects of retinoic acid were impaired in GPx3-inactivated myoblasts, which indicates that GPx3 regulates the antioxidative effects of retinoic acid. Therefore, retinoid status and GPx3 levels may have important implications for the viability of human muscle stem cells.
Structure, innervation and response properties of integumentary sensory organs in crocodilians
Authors: Leitch Duncan B. , Catania Kenneth C. 
Published: November 2012

SUMMARYIntegumentary sensory organs (ISOs) are densely distributed on the jaws of crocodilians and on body scales of members of the families Crocodilidae and Gavialidae. We examined the distribution, anatomy, innervation and response properties of ISOs on the face and body of crocodilians and documented related behaviors for an alligatorid (Alligator mississippiensis) and a crocodylid (Crocodylus niloticus). Each of the ISOs (roughly 4000 in A. mississippiensis and 9000 in C. niloticus) was innervated by networks of afferents supplying multiple different mechanoreceptors. Electrophysiological recordings from the trigeminal ganglion and peripheral nerves were made to isolate single-unit receptive fields and to test possible osmoreceptive and electroreceptive functions. Multiple small (&lt;0.1 mm2) receptive fields, often from a single ISO, were recorded from the premaxilla, the rostral dentary, the gingivae and the distal digits. These responded to a median threshold of 0.08 mN. The less densely innervated caudal margins of the jaws had larger receptive fields (&gt;100 mm2) and higher thresholds (13.725 mN). Rapidly adapting, slowly adapting type I and slowly adapting type II responses were identified based on neuronal responses. Several rapidly adapting units responded maximally to vibrations at 20–35 Hz, consistent with reports of the ISOs' role in detecting prey-generated water surface ripples. Despite crocodilians' armored bodies, the ISOs imparted a mechanical sensitivity exceeding that of primate fingertips. We conclude that crocodilian ISOs have diverse functions, including detection of water movements, indicating when to bite based on direct contact of pursued prey, and fine tactile discrimination of items held in the jaws.
Dine or dash? Turbulence inhibits blue crab navigation in attractive–aversive odor plumes by altering signal structure encoded by the olfactory pathway
Authors: Weissburg Marc , Atkins Lorin , Berkenkamp Kimberly , Mankin Danielle 
Published: November 2012

SUMMARYBlue crabs can distinguish and navigate to attractive (food) odors even when aversive odors (injured crab metabolites) are released nearby. Blue crabs in these conditions detect the aversive odor and avoid it, but find the attractive source with nearly the same success rate as when the attractive source is presented alone. Spatially and temporally distinct odor filaments appear to signal to foragers that the two odor sources are not co-located, and hence navigating to the attractive odor entails an acceptable risk of predation. However, environmentally produced turbulence suppresses tracking by homogenizing the two odors; blue crabs fail to track to the attractive source when the aversive source is present, even though turbulence does not substantially inhibit tracking to the attractive source alone. Removal of sensory input from aesthetascs on the antennules, but not chemosensors on the legs, rescues navigation to attractive–aversive dual plumes in turbulent conditions. These results suggest that mixing in the natural environment may amplify the effects of predators by suppressing tracking to food odors when aversive cues are present, and that the olfactory pathway mediates the response.
Use of a Fogarty catheter after tracheobronchial inhalation of a bead
Authors: Landy Christian , Massoure Pierre-Laurent , Gauthier Jerome , Eve Olivier , Kaiser Eric 
Published: November 2012

Summary We describe a case of tracheobronchial inhalation of a bead by a five-year-old girl in Djibouti. The bead was extracted using a Fogarty balloon catheter passed through the rigid bronchoscope without complication. This kind of accident is not exceptional and we discuss the management of such cases with limited diagnostic tools and therapeutic environments.
Novel H6PDH mutations in two girls with premature adrenarche: ‘apparent’ and ‘true’ CRD can be differentiated by urinary steroid profiling
Authors: Lavery G G , Idkowiak J , Sherlock M , Bujalska I , Ride J P , Saqib K , Hartmann M F , Hughes B , Wudy S A , De Schepper J , Arlt W , Krone N , Shackleton C H , Walker E A , Stewart P M 
Published: November 2012

ContextInactivating mutations in the enzyme hexose-6-phosphate dehydrogenase (H6PDH, encoded by H6PD) cause apparent cortisone reductase deficiency (ACRD). H6PDH generates cofactor NADPH for 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1, encoded by HSD11B1) oxo-reductase activity, converting cortisone to cortisol. Inactivating mutations in HSD11B1 cause true cortisone reductase deficiency (CRD). Both ACRD and CRD present with hypothalamic-pituitary-adrenal (HPA) axis activation and adrenal hyperandrogenism.ObjectiveTo describe the clinical, biochemical and molecular characteristics of two additional female children with ACRD and to illustrate the diagnostic value of urinary steroid profiling in identifying and differentiating a total of six ACRD and four CRD cases.DesignClinical, biochemical and genetic assessment of two female patients presenting during childhood. In addition, results of urinary steroid profiling in a total of ten ACRD/CRD patients were compared to identify distinguishing characteristics.ResultsCase 1 was compound heterozygous for R109AfsX3 and a novel P146L missense mutation in H6PD. Case 2 was compound heterozygous for novel nonsense mutations Q325X and Y446X in H6PD. Mutant expression studies confirmed loss of H6PDH activity in both cases. Urinary steroid metabolite profiling by gas chromatography/mass spectrometry suggested ACRD in both cases. In addition, we were able to establish a steroid metabolite signature differentiating ACRD and CRD, providing a basis for genetic diagnosis and future individualised management.ConclusionsSteroid profile analysis of a 24-h urine collection provides a diagnostic method for discriminating between ACRD and CRD. This will provide a useful tool in stratifying unresolved adrenal hyperandrogenism in children with premature adrenarche and adult females with polycystic ovary syndrome (PCOS).
Phylogeny of Nearctic Pandemis (Lepidoptera: Tortricidae), with Focus on Species Boundaries in the P. limitata Group
Authors: Dombroskie Jason J , Sperling Felix A H 
Published: November 2012

Abstract
Despite their status as economically important pests on apple, species boundaries in the four native Nearctic species of Pandemis Hübner have remained uncertain for a century. We use a total evidence approach to test their genomic integrity using DNA (cytochrome oxidase I and ITS2), morphological (greyness of various wing areas), and geographic evidence. No single source of characters is sufficient, but combined evidence successfully separates the four species. We also examine the generic boundaries of Pandemis, using all Nearctic species, most western Palaearctic species, and a species of Archepandemis. DNA data place Archepandemis within Pandemis indicating that its members have lost the secondary sexual characters found in other Pandemis species.
Population Structure and Genetic Diversity of the Boll Weevil (Coleoptera: Curculionidae) onGossypiumin North America
Authors: Kuester Adam P , Jones Robert W , Sappington Thomas W , Kim Kyung Seok , Barr Norman B , Roehrdanz Richard L , Senechal Patti , Nason John D 
Published: November 2012

AbstractAlthough the boll weevil, Anthonomus grandis grandis Boheman (Coleoptera: Curculionidae), is a devastating pest in the United States and Mexico, its population structure and genetic diversity in Mexico on wild and cultivated cotton hosts (genus Gossypium) is poorly understood. Past studies using morphology, host use, and distribution records suggest that A.grandis grandis comprises three forms with host-associated characteristics: the southeastern form (from domesticated Gossypium hirsutum L., southeastern United States and northeastern Mexico), the thurberia form (from Gossypium thurberi Todaro, Arizona and northwestern Mexico), and the Mexican form (from multiple Gossypium species and other malvaceous plant genera in the remainder of Mexico and Central America). However, the phylogenetic relationships, host preferences, and distributions of these forms are not completely understood. An alternative hypothesis of an eastern and western form of the boll weevil is suggested by the suspected phylogeographic range expansion from an ancestral distribution in the tropics northward along both Mexican coasts, culminating in the maximally contrasting phenotypes observed in the northeastern and northwestern arms of the current distribution. In this study, we sequenced one mitochondrial and four nuclear genes to gain insight into the evolutionary relationships among the putative forms and their distributions on wild and domesticated cotton hosts. Using models of evolution, we compared the three-form to the two-form classification and to two alternative classifications that incorporate geography and host use traits. The genetic data at most loci provide stronger support for the two-form than the three-form hypothesis, with an eastern and western group separated by the Sierra Madre Occidental mountain range. They do not support separate taxonomic status for boll weevils developing on G.thurberi.
Medical Inpatients' Adherence to Outpatient Psychiatric Aftercare: A Prospective Study of Patients Evaluated by an Inpatient Consultation Liaison Psychiatry Service
Authors: Ehrenreich Mark J. , Robinson Charles T. , Glovinsky David B. , Dixon Lisa B. , Medoff Deborah R. , Himelhoch Seth S. 
Published: November 2012

Objective: This study sought to determine whether patients on psychiatric medication evaluated by inpatient consultation psychiatrists followed up with psychiatric aftercare and continued psychiatric medication 8 weeks post-discharge. Barriers to care and their effect on aftercare follow-up were assessed. Method: This was a prospective study of a consecutive sample of adults who received a psychiatric consultation and were prescribed psychotropic medication during hospitalization on the general medical or surgical inpatient units at the University of Maryland Medical Center. Baseline information was collected from 36 patients who received an inpatient psychiatric consultation and were: (1) prescribed psychiatric medications; and (2) discharged to home. Follow-up data was collected from 21 (58.3%) of these patients 8 weeks post-discharge. Results: Of 36 patients who provided baseline data, 93% recognized they had a psychiatric disorder, 90% recognized the importance of taking psychiatric medication, and 80% recognized the importance of psychiatric aftercare. Aftercare recommendations were included in only 33% of patient discharge instructions. Of 21 patients providing follow-up data, 57% reported receiving psychiatric aftercare. Patients who did not receive psychiatric aftercare were significantly more likely to be at risk for poor literacy (88.9% vs. 33.3% Fisher's exact test = 0.024) and were less often given psychiatric aftercare instructions at discharge (22% vs. 42%). Conclusions: Poor communication of aftercare instructions as well as poor literacy may be associated with lack of psychiatric aftercare. Consultation psychiatrists should assess literacy and insure aftercare information is provided to patients.
Factors Associated with Low Bone Density among Women with Major Depressive Disorder
Authors: Hsiao Mei-Chun , Liu Chia-Yih , Wang Chao-Jan 
Published: November 2012

Objective: Previous studies have suggested that depression might be associated with low bone mineral density (BMD) in women with depression. The aim of this study was to investigate the association between the BMD of women with major depressive disorder and correlated factors. Method: This prospective cross-sectional study explored the association between bone density and major depressive disorder in women. One hundred women diagnosed with major depressive disorder were enrolled. The diagnoses were made by board-certificated psychiatrists using the Mini International Neuropsychiatric Interview (MINI). The Beck Depression Inventory (BDI) was administered. The bone density of the hip was measured with dual X-ray densitometry (DEXA) using a Hologic Delphi QDR-2000 densitometer. Results: We found age, family history of osteoporosis, consumption of coffee, and consumption of tea to be associated with low BMD in single-variate analysis. Depression was also related to BMD, in that the worse the depression, the lower the BMD. Multi-variate analysis by linear regression revealed an equation of BMD = 0.91 − 0.004 × (severity of depression) + 0.07 × (tea consumption) − 0.06 × (family history of osteoporosis) − 0.04 × age. Conclusion: These results suggest that depression is associated with lower BMD, and the associated factors should be considered in depressive women. The findings of this research may be useful for improving the care of women with major depressive disorder in terms of developing appropriate and effective care plans.
Mega-Melodrama! Vertical and Horizontal Suspensions of the “Classical”
Authors: Williams Linda 
Published: November 2012

ABSTRACT:“Mega-melodrama!” describes blockbuster action films that occupy more and different kinds of space than previous films and serial television programs that occupy more and different time than previous episodic television. This article defines melodrama anew and attempts to disabuse scholars of a habit of thought in film studies that consistently aligns melodrama – whether action blockbusters or more emotionally resonant “women’s films” – with stylistic or emotional excess and thus, implicitly or explicitly, opposes it to a “classical” norm. For what we habitually call the classical is even more protean than the supposed excess of melodrama. Indeed, the very norms of melodrama are what we often mistake for the classical.