bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leaves to Measure Light Intensity
Authors: Lahlou Aliénor , Coghill Ian , Davidson Mhairi L. H. , Billon Romain , Barneche Fredy , Lazar Dusan , Le Saux Thomas , Jullien Ludovic 
Published: July 2024

AbstractQuantitative measurement of light intensity is a key step in ensuring the reliability and the reproducibility of scientific results in many fields of physics, biology, and chemistry. The protocols presented so far use various photoactive properties of manufactured materials. Here, leaves are introduced as an easily accessible green material to calibrate light intensity. The measurement protocol consists in monitoring the chlorophyll fluorescence of a leaf while it is exposed to a jump of constant light. The inverse of the characteristic time of the initial chlorophyll fluorescence rise is shown to be proportional to the light intensity received by the leaf over a wide range of wavelengths and intensities. Moreover, the proportionality factor is stable across a wide collection of plant species, which makes the measurement protocol accessible to users without prior calibration. This favorable feature is finally harnessed to calibrate a source of white light from exploiting simple leaves collected from a garden.
Precise Identification of Glioblastoma Micro‐Infiltration at Cellular Resolution by Raman Spectroscopy
Authors: Zhu Lijun , Li Jianrui , Pan Jing , Wu Nan , Xu Qing , Zhou Qing‐Qing , Wang Qiang , Han Dong , Wang Ziyang , Xu Qiang , Liu Xiaoxue , Guo Jingxing , Wang Jiandong , Zhang Zhiqiang , Wang Yiqing , Cai Huiming , Li Yingjia , Pan Hao , Zhang Longjiang , Chen Xiaoyuan , Lu Guangming 
Published: July 2024

AbstractPrecise identification of glioblastoma (GBM) microinfiltration, which is essential for achieving complete resection, remains an enormous challenge in clinical practice. Here, the study demonstrates that Raman spectroscopy effectively identifies GBM microinfiltration with cellular resolution in clinical specimens. The spectral differences between infiltrative lesions and normal brain tissues are attributed to phospholipids, nucleic acids, amino acids, and unsaturated fatty acids. These biochemical metabolites identified by Raman spectroscopy are further confirmed by spatial metabolomics. Based on differential spectra, Raman imaging resolves important morphological information relevant to GBM lesions in a label‐free manner. The area under the receiver operating characteristic curve (AUC) for Raman spectroscopy combined with machine learning in detecting infiltrative lesions exceeds 95%. Most importantly, the cancer cell threshold identified by Raman spectroscopy is as low as 3 human GBM cells per 0.01 mm2. Raman spectroscopy enables the detection of previously undetectable diffusely infiltrative cancer cells, which holds potential value in guiding complete tumor resection in GBM patients.
The placebo effect of sham rhinologic procedures in randomized controlled trials: A systematic review and meta‐analysis
Authors: Monaghan Neil P. , Duckett Kelsey A. , Nguyen Shaun A. , Massey Abigail A. , Rathi Vinay , Soler Zachary M. , Schlosser Rodney J. 
Published: July 2024

Key points
Sham procedures produce a clinically significant impact on patient‐reported outcome measures
This effect should be considered when designing and interpreting the results of RCTs
Reduction‐Induced Self‐Propelled Oscillatory Motion of Perylenediimides on Water
Authors: Holstein Lara Rae , Suematsu Nobuhiko J. , Takeuchi Masayuki , Harano Koji , Banno Taisuke , Takai Atsuro 
Published: July 2024

AbstractThe emergence of macroscopic self‐propelled oscillatory motion based on molecular design has attracted continual attention in relation to autonomous systems in living organisms. Herein, a series of perylenediimides (PDIs) with various imide side chains was prepared to explore the impact of molecular design and alignment on the self‐propelled motion at the air‐water interface. When placed on an aqueous solution containing a reductant, a solid disk of neutral PDI was reduced to form the water‐soluble, surface‐active PDI dianion species, which induces a surface tension gradient in the vicinity of the disk for self‐propelled motion. We found that centimeter‐scale oscillatory motion could be elicited by controlling the supply rate of PDI dianion species through the reductant concentration and the structure of the imide side chains. Furthermore, we found that the onset and speed of the self‐propelled motion could be changed by the crystallinity of PDI at the water surface. This design principle using π‐conjugated molecules and their self‐assemblies could advance self‐propelled, non‐equilibrium systems powered by chemical energy.
A Comprehensive Review of CO2 Hydrogenation into Formate/Formic Acid Catalyzed by Whole Cell Bacteria
Authors: Moniruzzaman Mohammad , Afrin Sadia , Hossain Saddam , Yoon Ki‐Seok 
Published: July 2024

AbstractThe increasing levels of carbon dioxide (CO2) in the atmosphere, primarily due to the use of fossil fuels, pose a significant threat to the environment and necessitate urgent action to mitigate climate change. Carbon capture and utilization technologies that can convert CO2 into economically valuable compounds have gained attention as potential solutions. Among these technologies, biocatalytic CO2 hydrogenation using bacterial whole cells shows promise for the efficient conversion of CO2 into formate, a valuable chemical compound. Although it was discovered nearly a century ago, comprehensive reviews focusing on the utilization of whole‐cell bacteria as the biocatalyst in this area remain relatively limited. Therefore, this review provides an analysis of the progress, strategies, and key findings in this field. It covers the use of living cells, resting cells, or genetically modified bacteria as biocatalysts to convert CO2 into formate, either naturally or with the integration of electrochemical and protochemical techniques as sources of protons and electrons. By consolidating the current knowledge in this field, this review article aims to serve as a valuable resource for researchers and practitioners interested in understanding the recent progress, challenges, and potential applications of bacterial whole cell catalyzed CO2 hydrogenation into formate.
Microbial synthesis of gallic acid and its glucoside β‐glucogallin
Authors: Guo Jiao , Ren Xiang , Lu Liangyu , An Ning , Li Shuwei , Geng Mingjie , Li Geng , Shen Xiaolin , Sun Xinxiao , Wang Jia , Yuan Qipeng 
Published: July 2024

AbstractGallic acid (GA) and β‐glucogallin (BGG) are natural products with diverse uses in pharmaceutical, food, chemical and cosmetic industries. They are valued for their wide‐ranging properties such as antioxidant, antibacterial, antidiabetic, and anticancer properties. Despite their significant importance, microbial production of GA and BGG faces challenges such as limited titers and yields, along with the incomplete understanding of BGG biosynthesis pathways in microorganisms. To address these challenges, we developed a recombinant Escherichia coli strain capable of efficiently producing GA. Our approach involved screening efficient pathway enzymes, integrating biosynthetic pathway genes into the genome while balancing carbon flux via adjusting expression levels, and strengthening the shikimate pathway to remove bottlenecks. The resultant strain achieved impressive results, producing 51.57 g/L of GA with a carbon yield of 0.45 g/g glucose and a productivity of 1.07 g/L/h. Furthermore, we extended this microbial platform to biosynthesize BGG by screening GA 1‐O‐glucosyltransferase, leading to the de novo production of 92.42 mg/L of BGG. This work establishes an efficient chassis for producing GA at an industrial level and provides a microbial platform for generating GA derivatives.
Optimizing virus filtration for continuous processing using serial filtration at high area ratio
Authors: Giglia Sal , Cacace Ben , McCoskey Jacob , Tierson Matthew , Carbrello Christina , Miller Corinne , Hersey Joseph 
Published: July 2024

AbstractCompared to batch operation, continuous bioprocessing can offer numerous advantages, including increased productivity, improved process control, reduced footprint, and increased flexibility. However, integration of traditional batch operations into a connected process can be challenging. In contrast to batch operations run at constant pressure or high flux, virus filtration in continuous processes may be operated at very low flux. This change in operating conditions may reduce the viral retention performance of the filter which has inhibited adoption of truly continuous virus filtration. To overcome this limitation, a novel approach is described that utilizes serial virus filtration, with a high area ratio between first to second stage filters, to achieve virus retention targets. In this study, virus filters were operated continuously (except for planned process interruptions) for 200 h in a serial configuration at a first to second stage filter area ratio of 13:1 and at a first stage flux of 5 L/m2/h. While the minute virus of mice (MVM) retention performance of the first stage filter was about 4 log reduction value (LRV), there was no virus detected in the second stage filtrate, translating to an MVM LRV across the filtration train of ≥6.7. The second stage filter was the dominant flow resistance at the start of the run but, as it was protected from foulants by the first stage filter, it suffered minimal fouling and the life of the filter train was controlled by the first stage. A theoretical case study projected that continuous virus filtration using serial configuration at high area ratio would have about 30% longer filter changeout time, 14% higher productivity, and virus retention nearly six LRV greater than single stage operation. The findings of this research are expected to provide valuable insights into optimizing virus filtration in continuous bioprocessing.
Chemical Profiling and Cytotoxicity Activity of Essential Oil from Artemisia Maritima Collected through Four Different Wild Locations of Lahaul Valley in Indian Western Himalaya
Authors: Pathania Vijaylata , Vashisath Sachin , Bhardwaj Rishabh , Kapoor Smita , Kumar Dinesh 
Published: July 2024

AbstractThis research leads to the analysis of quality parameters and chemical profiles of Artemisia maritima essential oils (EOs) extracted from four different wild areas viz: Darcha, Jispa, Gemur and Tandi of Lahaul valley of Indian western Himalaya in comparison to a market sample. The EOs content was found higher in Darcha (0.54 %) and Jispa (0.48 %). The quality parameters such as color, odor, density, refractive index and optical rotations were found comparable with the market sample. Further, volatile chemo‐profile of EOs was generated using GC/GC‐MS. A total of 34 metabolites were identified in five samples (n=3) that account around 87.3–95.1 % of the total oils. These EOs majorly contained monoterpene hydrocarbons (3.0–68.5 %) and oxygenated monoterpenes (26.6–85.0 %) with β‐myrcene (9.8–20.6 %), α‐terpinene (0.7–17.3 %), β‐phellandrene (1.5–22.4 %), 1,8‐cineole (3.0–50.8 %), terpinen‐4‐ol (2.5–7.2 %), ascaridole (11.5 %), bornyl acetate (5.1–14.2 %), trans‐sabinyl acetate (0.8–49.6) and trans‐ascaridole (7.9 %). Moreover, cytotoxicity study of EOs against CaCo2 and HT‐29 human colon carcinoma cell lines unveiled significant anticancer potential of A. maritima. AMD (82.87±0.39 %) AMG (61.17±1.45 %) and AMM (73.37±2.1 %) showed comparable activity at 600 μg/ml (72 hr) in comparisons to vinblastine (20 μM). A. maritima is less explored as compared to other species and could be a new potential source for EOs and anticancer candidate.
Overexpression of EGFL7 promotes angiogenesis and nerve regeneration in peripheral nerve injury
Authors: Hao Zengtao , Huo Zhiqi , Aixin‐Jueluo Qicheng , Wu Tao , Chen Yihong 
Published: July 2024

AbstractPeripheral nerve injury (PNI) often leads to significant functional impairment. Here, we investigated the impact of epidermal growth factor‐like domain‐containing protein 7 (EGFL7) on angiogenesis and nerve regeneration following PNI. Using a sciatic nerve injury model, we assessed nerve function using the sciatic nerve function index. We analyzed the expression levels of EGFL7, forkhead box proteins A1 (FOXA1), nerve growth factor (NGF), brain‐derived neurotrophic factors (BDNF), Neurofilament 200 (NF200), myelin protein zero (P0), cell adhesion molecule 1 (CD31), vascular endothelial growth factor (VEGF), and NOTCH‐related proteins in tissues and cells. Cell proliferation, migration, and angiogenesis were evaluated through cell counting kit assays, 5‐ethynyl‐2′deoxyuridine staining, and Transwell assays. We investigated the binding of FOXA1 to the EGFL7 promoter using dual‐luciferase assays and chromatin immunoprecipitation. We observed decreased EGFL7 expression and increased FOXA1 expression in PNI, and EGFL7 overexpression alleviated gastrocnemius muscle atrophy, increased muscle weight, and improved motor function. Additionally, EGFL7 overexpression enhanced Schwann cell and endothelial cell proliferation and migration, promoted tube formation, and upregulated NGF, BDNF, NF200, P0, CD31, and VEGF expression. FOXA1 was found to bind to the EGFL7 promoter region, inhibiting EGFL7 expression and activating the NOTCH signaling pathway. Notably, FOXA1 overexpression counteracted the effects of EGFL7 on Schwann cells and endothelial cells. In conclusion, EGFL7 holds promise as a therapeutic molecule for treating sciatic nerve injury.
Optimizing delignification and saccharification process for sawdust processing using a central composite design
Authors: Yadav Priya , Kring Julie , Gogate Parag R. 
Published: July 2024

AbstractLignocellulosic mass consists of cellulose, hemicelluloses, and lignin. Although biomass promises to be efficiently used for biofuel production and many other value‐added products, lignin present in lignocellulosic biomass affects the hydrolysis of cellulose and hemicelluloses, making it necessary to develop techniques that provide better lignin removal efficiency and high cellulose hydrolysability. The current work aims to maximize lignin removal in sawdust and develop an understanding of the hydrolysis of pretreated biomass for sugar production. Different parameters such as solvent to solid ratio, temperature, and reaction time have been considered based on the design of experiment to understand the effect on the delignification and saccharification processes. After treating sawdust for 1.5 h, it was observed that a maximum of 85% lignin was removed at a temperature of 131°C and solid loading of 16 g. Subsequent hydrolysis of delignified sawdust at 131°C temperature, solvent to solid ratio 15, and 0.5 h resulted in a maximum reducing sugar production of 26.82 mg/mL. The study elucidated the optimum conditions for the effective processing of sawdust in terms of delignification and saccharification, leading to maximum benefits in lignin removal and sugar production.
Revisiting the effectiveness of cognitive‐behavioural therapy for reducing reoffending in the criminal justice system: A systematic review
Authors: Smith Andrew , Roberts Anton , Krzemieniewska‐Nandwani Karolina , Eggins Elizabeth , Cook Will , Fox Chris , Maruna Shadd , Wallace Stephanie , Szifris Kirstine 
Published: July 2024

AbstractThis is the protocol for a Campbell systematic review. The objectives are as follows. The proposed systematic review is an update to, and extension of, Lipsey et al. (2007). As such we build on their previous aims to: (i) Assess and synthesise the overall impact of cognitive behavioural therapy (CBT) on offender recidivism; (ii) Examine possible sources of variability in the effectiveness of CBT. Data permitting, we will examine if the effectiveness of CBT varies by: (a) Characteristics of the CBT intervention (e.g., cognitive restructuring vs. cognitive skills training, group v. individual implementation; and/or custodial v. community setting, and/or), (b) Characteristics of the population (e.g., juveniles vs. adult offenders), (c) Implementation factors (e.g., implementing practitioner, use of structured/manualised approaches, delivery mode, and/or programme duration or intensity), (d) Evaluation methods (e.g., randomised vs. non‐randomised research designs); (iv) Determine whether there is a decline in the effect of CBT on recidivism over time; and (v) Investigate whether there is an interaction between implementation factors and time in terms of the effect on recidivism.
A Systematic Review of Momentary Social Predictors of Positive Psychosis Experiences
Authors: Irwin Leanne , Udachina Alisa , Bell Tobyn , Carter Lesley‐Anne , Handerer Fritz , Berry Katherine 
Published: July 2024

ABSTRACTBackgroundSocial factors can play an important role in the development and maintenance of psychosis. Clarifying this relationship is vital for advancing theoretical understanding and development of targeted interventions. Psychosis is increasingly researched with an experience sampling methodology (ESM), which provides an ecologically valid approach, that reduces recall biases. Studies examining momentary associations between social factors and psychosis have not yet been summarised.MethodWe identified 29 ESM studies investigating associations between social factors and positive psychotic experiences through a pre‐registered systematic search of the published literature.ResultsBeing alone did not predict increase in psychosis; however, appraisals and feelings associated with being alone such as feeling socially disconnected, lonely and unwanted did. Being with familiar company was found to reduce psychosis experiences but feeling stressed by the current company increased psychosis.ConclusionsWhile issues with sample size and generalisability mean these results should be interpreted with caution, some putative conclusions can be made. Individuals with psychosis or emerging symptoms should be offered interventions that improve social networks such as peer support, community participation and engagement skills training. These individuals may also benefit from virtual reality or compassion‐based interventions which aim to dampen perceived social threat. Moreover, digital interventions which monitor changes in social variables that predict relapse in symptoms would allow early intervention to prevent mental health crises.
Detuning analysis and power tracking of dual‐ended resonant circuit based on improved variable‐step perturbation observation for wireless power transfer system
Authors: Li Jiangui , Luo Guangbin , Wang Longyang , Si Qinghe , Peng Yinchong , Guo Zheyuan 
Published: July 2024

AbstractA power tracking method based on improved variable‐step perturbation observation approach has been proposed in this paper. This method is aimed at addressing the detuning issues caused by capacitor parameter drift in MCR‐WPT systems based on S‐S compensation circuits. Compared with traditional tuning methods, the proposed method has fast response, high accuracy, low complexity, and less prone to over‐tracking. Firstly, a mathematical model of the system based on the detuning factor has been established. Secondly, the impact of different detuning conditions on the system at the initial resonant frequency has been studied. Thirdly, the response characteristics of the system to different frequencies under different detuning conditions have been studied. Fourthly, based on the above researches, an improved variable‐step perturbation observation method based on the single‐step power drop factor has been proposed. Finally, an experimental platform was constructed, and relevant experiments were conducted. Experimental results validate the effectiveness of power tracking under different detuning conditions, with the lowest transmission efficiency being 81.51%.
A benefit–risk analysis for biological control introductions based on the protection of native biodiversity
Authors: Heimpel George E. , Abram Paul K. , Causton Charlotte E. , Celis Sabrina L. , Coll Moshe , Hardy Ian C. W. , Mangel Marc , Mills Nicholas J. , Segoli Michal 
Published: July 2024

AbstractThe release of biological control agents has been an important means of controlling invasive species for over 150 years. While these releases have led to the sustainable control of over 250 invasive pest and weed species worldwide, a minority have caused environmental harm. A growing recognition of the risks of biological control led to a focus on risk assessment beginning in the 1990s along with a precipitous decline in releases. While this new focus greatly improved the safety of biological control, it came at the cost of lost opportunities to solve environmental problems associated with invasive species. A framework that incorporates benefits and risks of biological control is thus needed to understand the net environmental effects of biological control releases. We introduce such a framework, using native biodiversity as the common currency for both benefits and risks. The model is based on interactions among four categories of organisms: (1) the biological control agent, (2) the invasive species (pest or weed) targeted by the agent, (3) one or more native species that stand to benefit from the control of the target species, and (4) one or more native species that are at risk of being harmed by the released biological control agent. Conservation values of the potentially benefited and harmed native species are incorporated as well, and they are weighted according to three axes: vulnerability to extinction, the ecosystem services provided, and cultural significance. Further, we incorporate the potential for indirect risks to native species, which we consider will result mainly from the ecological process of agent enrichment that may occur if the agent exploits but does not control the target pest or weed. We illustrate the use of this framework by retrospectively analyzing the release of the vedalia beetle, Novius (= Rodolia) cardinalis, to control the cottony cushion scale, Icerya purchasi, in the Galapagos Islands. While the framework is particularly adaptable to biological control releases in natural areas, it can also be used in managed settings, where biological control protects native species through the reduction of pesticide use.
Population bottlenecks and sexual recombination shape diatom microevolution
Authors: Hay Mele Bruno , Ruggiero Maria Valeria , D'Alelio Domenico 
Published: July 2024

AbstractDiatoms are single‐celled organisms that contribute approximately 20% of the global primary production and play a crucial role in biogeochemical cycles and trophic chains. Despite their ecological importance, our knowledge of microevolution is limited. We developed a model using the SLiM evolutionary framework to address this knowledge gap. As a reference, we used the diatom Pseudo‐nitzschia multistriata, which has been extensively studied in the Gulf of Naples. Our model recapitulates what we observe in natural populations, with microevolutionary processes that occur annually during a three‐stage bloom phase. Interestingly, we found that non‐bloom phases allow the population to maintain sex‐generated diversity produced during blooms. This finding suggests that non‐bloom phases are critical to counteract bloom‐related pressures and mitigate genetic divergence at the species level. Moreover, our model showed that despite the consistent genetic differentiation during bloom phases, the population tends to return to pre‐bloom states. While our model is limited to neutral dynamics, our study provides valuable insights into diatoms' microevolution, paving the way to explore the ecological implications of the life history dynamics of these organisms.