bluuebunny/crossref_metadata_2025_split huggingface dataset:
KIR2DL2/DL3+NKs and Helios+Tregs in Peripheral Blood Predict Nivolumab Response in Patients with Metastatic Renal Cell Cancer
Authors: Santagata Sara , Trotta Anna Maria , D’Alterio Crescenzo , Napolitano Maria , Rea Giuseppina , Di Napoli Marilena , Portella Luigi , Ieranò Caterina , Guardascione Giuseppe , Coppola Elisabetta , Caux Christophe , Dubois Bertrand , Boyle Helen J. , Carles Joan , Rossetti Sabrina , Azzaro Rosa , Feroce Florinda , Perdonà Sisto , Fordellone Mario , Bello Anna Maria , Califano Daniela , Chiodini Paolo , Pignata Sandro , Scala Stefania 
Published: August 2024

Abstract

Purpose:
To identify predictive factors of nivolumab sensitivity, peripheral blood NKs and regulatory T-cell (Treg) were evaluated in patients with metastatic renal cell carcinoma (mRCC) enrolled in the REVOLUTION trial.

Experimental Design:
Fifty-seven mRCCs being treated with nivolumab, as at least second-line of therapy, and 62 healthy donors were longitudinally evaluated (0–1–3–6–12 months) for peripheral NKs and Tregs, phenotype, and function. Multivariable logistic regression was conducted to identify the independent predictors. The 0.632+ internal cross-validation was used to avoid overfitting. The best cutoff value based on a 3-month clinical response was applied to progression-free survival (PFS) and overall survival (OS). Kaplan–Meier curves for PFS and OS were produced.

Results:
At pretreatment, mRCCs displayed high frequency of NKp46+NKs, NKp30+NKs, KIR2DL1+NKs, KIR2DL2/DL3+NKs, and PD1+NKs with reduced NK degranulation as well as high frequency of Tregs, PD1+Tregs, Helios+Tregs, and ENTPD1+Tregs. Responder patients, identified as a clinical response after 3 months of treatment, presented at pretreatment significantly low CD3+, high KIR2DL2/DL3+NKs, high PD1+Tregs, and high Helios+Tregs. Upon multivariate analysis, only KIR2DL2/DL3NKs and Helios+Tregs held as independent predictors of nivolumab responsiveness. The KIR2DL2/DL3+NKs &gt;35.3% identified patients with longer OS, whereas the Helios+Tregs &gt;34.3% displayed significantly longer PFS. After 1-month of nivolumab, responder patients showed low CD3+, high NKs, KIR2DL2/DL3+NKs, and ICOS+Tregs. Among these subpopulations, CD3+ and KIR2DL2/DL3+NKs held as independent predictors of nivolumab efficacy. Low CD3+ (≤71%) was significantly associated with longer PFS, whereas high KIR2DL2/DL3+NKs (&gt;23.3%) were associated with both PFS and OS.

Conclusions:
Pretreatment evaluation of Helios+Tregs/KIR2DL2/DL3+NKs and 1-month posttreatment CD3+/ KIR2DL2/DL3+NKs will predict nivolumab response in mRCCs.
Hello, is it me you’re Stopping for? The Effect of external Human Machine Interface Familiarity on Pedestrians’ Crossing Behaviour in an Ambiguous Situation
Authors: Lee Yee Mun , Sidorov Vladislav , Madigan Ruth , Garcia de Pedro Jorge , Markkula Gustav , Merat Natasha 
Published: August 2024

Objective We investigated how different deceleration intentions (i.e. an automated vehicle either decelerated for leading traffic or yielded for pedestrians) and a novel (Slow Pulsing Light Band – SPLB) or familiar (Flashing Headlights – FH) external Human Machine Interface (eHMI) informed pedestrians’ crossing behaviour. Background The introduction of SAE Level 4 Automated Vehicles (AVs) has recently fuelled interest in new forms of explicit communication via eHMIs, to improve the interaction between AVs and surrounding road users. Before implementing these eHMIs, it is necessary to understand how pedestrians use them to inform their crossing decisions. Method Thirty participants took part in the study using a Head-Mounted Display. The independent variables were deceleration intentions and eHMI design. The percentage of crossings, collision frequency and crossing initiation time across trials were measured. Results Pedestrians were able to identify the intentions of a decelerating vehicle, using implicit cues, with more crossings made when the approaching vehicles were yielding to them. They were also more likely to cross when a familiar eHMI was presented, compared to a novel one or no eHMI, regardless of the vehicle’s intention. Finally, participants learned to take a more cautious approach as trials progressed, and not to base their decisions solely on the eHMI. Conclusion A familiar eHMI led to early crossings regardless of the vehicle’s intention but also led to a higher collision frequency than a novel eHMI. Application To achieve safe and acceptable interactions with AVs, it is important to provide eHMIs that are congruent with road users’ expectations.
An Overview of Indian Marine Seaweeds and their Medicinal Applications
Authors: R. Kavya , Ananthy Vimala , Salwe Kartik Janardan , Manimekalai K. , Komarasamy Boopathi , Deva Reka 
Published: August 2024

Background Seaweeds are macroalgae containing bioactive compounds that have positively impacted the biomedical field due to polysaccharides, sterols and glycolipids, showing a diverse range of pharmacological and biological properties. Eating a diet high in marine items will lessen the risk of developing diabetes, cancer and obesity. Purpose The exploration of medically useful seaweeds is required to identify drugs and drug targets and reduce the risk of developing diseases. Methodology The PubMed search was done with the MESH terms such as seaweed and Marine algae pharmacological activity results were obtained as 748 results. We filtered out the articles and entered key terms related to our area of interest to find the results. The seaweed species in India are listed with Tamil Nadu contributing the most and Orissa the least. The type of algae is also mentioned. Results Marine algae have been employed in industry and medicine as a novel food with possible nutritional benefits. Furthermore, it has been demonstrated that marine algae are a rich source of naturally occurring bioactive substances with activities such as anti-diabetic, hepatoprotective, antiviral, antifungal, antibacterial, antimicrobial, antioxidant, anti-inflammatory, anti-hypercholesterolemia and hypolipidemia. Conclusion The current review focuses on the bioactivities and potential pharmaceutical activity of marine algae discovered in India. The present review article showed that there are many seaweeds of medical importance, and it is necessary to explore them to minimize disease progression and prolong the individual’s survival.
Should I prescribe exercise to a patient with an immobilized shoulder? An electromyographic study in patients with unilateral shoulder pain and healthy volunteers
Authors: Intelangelo Leonardo , Ignacio Lassaga , Mendoza Cristian , Roulet Iván , Jerez-Mayorga Daniel , Barbosa Alexandre Carvalho 
Published: August 2024

Background There is limited evidence about exercise performance during postoperative immobilization. This study aimed to quantify the muscle activity in the immobilized shoulder girdle in an abduction and neutral rotation position during a battery of contralateral activities and ipsilateral exercises of the upper limb in healthy volunteers and patients with unilateral shoulder pain (USP). Methods Thirty participants were included and grouped as without ( N = 17) or with USP ( N = 13). Muscle activity (percentage of maximum voluntary isometric contraction (%MVIC)) of the serratus anterior (SA), upper trapezius (UT), lower trapezius (LT) and infraspinatus muscles was assessed during five contralateral activities (during comfortable speed, fast speed and comfortable speed with additional load) and seven ipsilateral exercises. Results No differences in % MVIC were found between healthy volunteers and patients with USP on immobilized upper limb exercises and with the contralateral shoulder girdle. General muscle activity was low (<15% MVIC) to moderate (21–40% MVIC). This similarity was maintained at different speeds and with additional load. Adapted lawnmower exercise showed a favorable UT/LT activation ratio. Conclusion The study results support the use of all evaluated shoulder exercises, as they have shown low muscle activity, which is important for early rehabilitation phases.
Utility of 3D-Printed Models in the Surgical Planning for Primary Spine Tumors: A Survey of International Spinal Oncology Experts
Authors: Mikula Anthony L. , Rhines Laurence D. , Patel Shalin S. , Pennington Zach , Karim S. Mohammed , Morris Jonathan M. , Tappa Karthik , Alvarez-Breckenridge Christopher , North Robert Y. , Tatsui Claudio E. , Rose Peter S. , Clarke Michelle J. , Bird Justin E. 
Published: August 2024

Study Design Survey study. Objectives The purpose of this study was to characterize the utility of 3D printed patient specific anatomic models for the planning of complex primary spine tumor surgeries. Methods A survey of individual members of an international study group of spinal oncology surgeons was performed. Participants were provided a clinical vignette, pathologic diagnosis, and pre-operative imaging for three primary spinal oncology cases. Study participants provided a free text surgical plan for resection and were then presented an associated 3D printed model for each case and asked to re-evaluate their surgical plan. Results Ten spinal oncology surgeons participated in the study, representing nine institutions across five countries. Four of the surgeons (40%) made significant changes to their surgical plan after reviewing the 3D models, including sacrifice of an additional nerve root to obtain negative margins, sparing an SI joint that was originally planned for inclusion in the en bloc resection, adjusting the location of osteotomy cuts, changes to the number of surgical stages and/or staging order, and preservation of neurology that was originally planned for sacrifice. The overall impression of the 3D models was positive, with 90% of the participants stating they found the 3D model useful in developing a surgical plan. Conclusions Surgical planning for resection of primary spinal column tumors is challenging and time intensive. 3D printed patient specific surgical models may be an additional tool that can augment surgical planning and execution by improving the chance of accomplishing surgical resection goals and minimizing morbidity.
Adolescents’ Perceptions of Screening, Brief Intervention, and Referral to Treatment Service at Pediatric Trauma Centers
Authors: Mello Michael J , Baird Janette , Spirito Anthony , Scott Kelli , Zonfrillo Mark R , Lee Lois K , Kiragu Andrew , Christison-Lagay Emily , Bromberg Julie , Ruest Stephanie , Pruitt Charles , Lawson Karla A , Nasr Isam W , Aidlen Jeremy T , Maxson Robert Todd , Becker Sara 
Published: August 2024

Objective: Screening, brief intervention, and referral to treatment (SBIRT) for adolescent alcohol and drug (AOD) use is recommended to occur with adolescents admitted to pediatric trauma centers. Most metrics on SBIRT service delivery only reference medical record documentation. In this analysis we examined changes in adolescents’ perception of SBIRT services and concordance of adolescent-report and medical record data, among a sample of adolescents admitted before and after institutional SBIRT implementation. Methods: We implemented SBIRT for adolescent AOD use using the Science to Service Laboratory implementation strategy and enrolled adolescents at 9 pediatric trauma centers. The recommended clinical workflow was for nursing to screen, social work to provide adolescents screening positive with brief intervention and referral to their PCP for continued AOD discussions with those. Adolescents screening as high-risk also referred to specialty services. Adolescents were enrolled and contacted 30 days after discharge and asked about their perception of any SBIRT services received. Data were also extracted from enrolled patient’s medical record. Results: There were 430 adolescents enrolled, with 424 that were matched to their EHR data and 329 completed the 30-day survey. In this sample, EHR documented screening increased from pre-implementation to post-implementation (16.3%-65.7%) and brief interventions increased (27.1%-40.7%). Adolescents self-reported higher rates of being asked about alcohol or drug use than in EHR data both pre- and post-implementation (80.7%-81%). Both EHR data and adolescent self-reported data demonstrated low referral back to PCP for continued AOD discussions. Conclusions: Implementation of SBIRT at pediatric trauma centers was not associated with change in adolescent perceptions of SBIRT, despite improved documentation of delivery of AOD screening and interventions. Adolescents perceived being asked about AOD use more often than was documented. Referral to PCP or specialty care for continued AOD discussion remains an area of needed attention. Trial registration: Clinicaltrials.gov NCT03297060
Behavioral and Health Outcome Differences by Heroin or Methamphetamine Preference Among People in Rural US Communities Who Use Both Substances
Authors: Mixson L Sarah , Whitney Bridget M , Jenkins Wiley D , Stopka Thomas J , Korthuis P Todd , Drumright Lydia N , Ruderman Stephanie A , Friedmann Peter D , Pho Mai T , Young April M , Westergaard Ryan P , Seal David W , Go Vivian F , Miller William C , Zule William A , Feinberg Judith , Cooper Hannah LF , Tsui Judith I , Crane Heidi M , Delaney Joseph A 
Published: August 2024

Background: The United States’ (US) opioid overdose epidemic has evolved into a combined stimulant/opioid epidemic, a pattern driven in part by mitigating opioid overdose risk, variable substance availability, and personal preferences. This study aimed to investigate the association between self-reported substance preference (heroin or methamphetamine) and behavioral/health outcomes among individuals who used both heroin and methamphetamine in the rural US. Methods: The Rural Opioid Initiative is a consortium of 8 research cohorts from 10 states and 65 rural counties that recruited individuals reporting past 30-day injection of any substance or opioid substance use by any route from 1/2018 to 3/2020. Analyses were restricted to participants ⩾18 years, who self-reported either heroin or methamphetamine as their preferred substance and past 30-day use of both heroin and methamphetamine. We examined cross-sectional associations between preferred substance (heroin versus methamphetamine) and behavioral and health outcomes using random effects meta-analysis with adjusted regression models. Results: Among 1239 participants, 61% (n = 752) reported heroin as their preferred substance. Adjusting for age, sex, and race/ethnicity, methamphetamine preference was associated with lower prevalence ratios for current naloxone possession (adjusted prevalence ratio [aPR] = 0.68; 95% Confidence Interval [95% CI] = 0.59-0.78; P-value ⩽ .001), of ever being told they had the hepatitis C virus (HCV; aPR = 0.72; 95% CI: 0.61-0.85; P-value ⩽ .001) and a personal history of overdose (aPR = 0.81; 95% CI = 0.73-0.90; P-value ⩽ .001). Conclusion: In our study analyzing associations between preferred substance and various behavioral and health outcomes amongst people who use both heroin and methamphetamine, a majority of participants preferred heroin. Methamphetamine preference was associated with lower prevalence of naloxone possession, ever being told they had HCV, and prior history of an overdose. This study underscores the need for targeted harm reduction services for people who prefer methamphetamine in rural areas.
Low-energy and tunable LIF neuron using SiGe bandgap-engineered resistive switching transistor
Authors: Kim Yijoon , Kim Hyangwoo , Oh Kyounghwan , Park Ju Hong , Kong Byoung Don , Baek Chang-Ki 
Published: August 2024

AbstractWe have proposed leaky integrate-and-fire (LIF) neuron having low-energy consumption and tunable functionality without external circuit components. Our LIF neuron has a simple configuration consisting of only three components: one bandgap-engineered resistive switching transistor (BE-RST), one capacitor, and one resistor. Here, the crucial point is that BE-RST with a silicon–germanium heterojunction possesses an amplified hysteric current switching with a low latch-up voltage due to improved hole storage capability and impact ionization coefficient. Therefore, the proposed neuron utilizing BE-RST requires an energy consumption of 0.36 pJ/spike, which is approximately six times lower than 2.08 pJ/spike of pure silicon-RST based neuron. In addition, the spiking properties can be tuned by modulating the leakage rate and threshold through gate bias, which contributes to energy-efficient sparse-activity and high learning accuracy. As a result, our proposed neuron can be a promising candidate for executing various spiking neural network applications.
Marker effect p-values for single-step GWAS with the algorithm for proven and young in large genotyped populations
Authors: Leite Natália Galoro , Bermann Matias , Tsuruta Shogo , Misztal Ignacy , Lourenco Daniela 
Published: August 2024

Abstract
Background
Single-nucleotide polymorphism (SNP) effects can be backsolved from ssGBLUP genomic estimated breeding values (GEBV) and used for genome-wide association studies (ssGWAS). However, obtaining p-values for those SNP effects relies on the inversion of dense matrices, which poses computational limitations in large genotyped populations. In this study, we present a method to approximate SNP p-values for ssGWAS with many genotyped animals. This method relies on the combination of a sparse approximation of the inverse of the genomic relationship matrix ($${\mathbf{G}}_{\mathbf{A}\mathbf{P}\mathbf{Y}}^\mathbf{-1}$$

G

A
P
Y

-
1

) built with the algorithm for proven and young ($$\text{APY}$$
APY
) and an approximation of the prediction error variance of SNP effects which does not require the inversion of the left-hand side (LHS) of the mixed model equations. To test the proposed p-value computing method, we used a reduced genotyped population of 50K genotyped animals and compared the approximated SNP p-values with benchmark p-values obtained with the direct inverse of LHS built with an exact genomic relationship matrix ($${\mathbf{G}}^\mathbf{-1})$$

G

-
1

)

. Then, we applied the proposed approximation method to obtain SNP p-values for a larger genotyped population composed of 450K genotyped animals.

Results
The same genomic regions on chromosomes 7 and 20 were identified across all p-value computing methods when using 50K genotyped animals. In terms of computational requirements, obtaining p-values with the proposed approximation reduced the wall-clock time by 38 times and the memory requirement by ten times compared to using the exact inversion of the LHS. When the approximation was applied to a population of 450K genotyped animals, two new significant regions on chromosomes 6 and 14 were uncovered, indicating an increase in GWAS detection power when including more genotypes in the analyses. The process of obtaining p-values with the approximation and 450K genotyped individuals took 24.5 wall-clock hours and 87.66GB of memory, which is expected to increase linearly with the addition of noncore genotyped individuals.

Conclusions
With the proposed method, obtaining p-values for SNP effects in ssGWAS is computationally feasible in large genotyped populations. The computational cost of obtaining p-values in ssGWAS may no longer be a limitation in extensive populations with many genotyped animals.
Identification of the mode of evolution in incomplete carbonate successions
Authors: Hohmann Niklas , Koelewijn Joël R. , Burgess Peter , Jarochowska Emilia 
Published: August 2024

Abstract
Background
The fossil record provides the unique opportunity to observe evolution over millions of years, but is known to be incomplete. While incompleteness varies spatially and is hard to estimate for empirical sections, computer simulations of geological processes can be used to examine the effects of the incompleteness in silico.
We combine simulations of different modes of evolution (stasis, (un)biased random walks) with deposition of carbonate platforms strata to examine how well the mode of evolution can be recovered from fossil time series, and how test results vary between different positions in the carbonate platform and multiple stratigraphic architectures generated by different sea level curves.

Results
Stratigraphic architecture and position along an onshore-offshore gradient has only a small influence on the mode of evolution recovered by statistical tests. For simulations of random walks, support for the correct mode decreases with time series length.
Visual examination of trait evolution in lineages shows that rather than stratigraphic incompleteness, maximum hiatus duration determines how much fossil time series differ from the original evolutionary process. Gradual directional evolution is more susceptible to stratigraphic effects, turning it into punctuated evolution. In contrast, stasis remains unaffected.

Conclusions
• Fossil time series favor the recognition of both stasis and complex, punctuated modes of evolution.
• Not stratigraphic incompleteness, but the presence of rare, prolonged gaps has the largest effect on trait evolution. This suggests that incomplete sections with regular hiatus frequency and durations can potentially preserve evolutionary history without major biases. Understanding external controls on stratigraphic architectures such as sea level fluctuations is crucial for distinguishing between stratigraphic effects and genuine evolutionary process.
Remineralizing effect of NSF on artificial enamel caries
Authors: Mohamed Osama Safwat , Hall Mohamed Ashraf , Karawia Inas 
Published: August 2024

Abstract
Introduction
Nanotechnology offers new approaches and endless opportunities for remineralizing tooth decay without being toxic or causing allergies. This study aimed to determine the effect of nanosilver fluoride (NSF) on the remineralization potential of enamel caries-like lesions compared to 5% sodium fluoride varnish in permanent teeth.

Methods
Fifteen teeth (molars and premolars) were gathered, cleaned, and polished using a scaler. After sectioning the teeth mesiodistally and removing the roots, the thirty specimens were subjected to a demineralized solution to induce early enamel lesions and then assigned randomly into two equal groups. The test materials were applied, and then all the specimens were subjected to a pH cycling model for 30 days. DIAGNOdent and surface roughness were investigated, and an evaluation of the enamel Ca and P weight% for Ca/P ratio calculation was done using SEM-EDX to analyze the specimens at the end of the study. The data were analyzed using an independent t-test.

Results
The mean values for the DIAGNOdent measurements for NSF and NaF at baseline and after demineralization were not significantly different (p > 0.05). After treatment, NaF varnish showed a significantly higher mean DIAGNOdent measurement (11.8 ± 5.80) than NSF (4.7 ± 1.6). The mean surface roughness of the NaF group (1.64 ± 0.39) was much higher than NSF’s mean surface roughness (1.07 ± 0.21). Specimens treated with NSF had statistically significant smoother surfaces (p < 0.001). The NSF group had a higher mean Ca/P ratio (2.9 ± 0.35) than NaF (2.2 ± 0.11). This difference was statistically significant (p = 0.012).

Conclusion
The study reveals that nano silver fluoride is a more effective treatment than sodium fluoride varnish in enhancing teeth’s clinical characteristics, particularly in terms of mineral content and surface roughness, suggesting it could be an improved strategy to prevent dental caries and maintain enamel integrity.