bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Sister Chromatid Cohesion Pathway Suppresses Multiple Chromosome Gain and Chromosome Amplification
Authors: Covo Shay , Puccia Christopher M , Argueso Juan Lucas , Gordenin Dmitry A , Resnick Michael A 
Published: December 2013

AbstractGain or loss of chromosomes resulting in aneuploidy can be important factors in cancer and adaptive evolution. Although chromosome gain is a frequent event in eukaryotes, there is limited information on its genetic control. Here we measured the rates of chromosome gain in wild-type yeast and sister chromatid cohesion (SCC) compromised strains. SCC tethers the newly replicated chromatids until anaphase via the cohesin complex. Chromosome gain was measured by selecting and characterizing copper-resistant colonies that emerged due to increased copies of the metallothionein gene CUP1. Although all defective SCC diploid strains exhibited increased rates of chromosome gain, there were 15-fold differences between them. Of all mutants examined, a hypomorphic mutation at the cohesin complex caused the highest rate of chromosome gain while disruption of WPL1, an important regulator of SCC and chromosome condensation, resulted in the smallest increase in chromosome gain. In addition to defects in SCC, yeast cell type contributed significantly to chromosome gain, with the greatest rates observed for homozygous mating-type diploids, followed by heterozygous mating type, and smallest in haploids. In fact, wpl1-deficient haploids did not show any difference in chromosome gain rates compared to wild-type haploids. Genomic analysis of copper-resistant colonies revealed that the “driver” chromosome for which selection was applied could be amplified to over five copies per diploid cell. In addition, an increase in the expected driver chromosome was often accompanied by a gain of a small number of other chromosomes. We suggest that while chromosome gain due to SCC malfunction can have negative effects through gene imbalance, it could also facilitate opportunities for adaptive changes. In multicellular organisms, both factors could lead to somatic diseases including cancer.
Screening for Behavioral Health Issues in Children Enrolled in Massachusetts Medicaid
Authors: Hacker Karen A. , Penfold Robert , Arsenault Lisa , Zhang Fang , Murphy Michael , Wissow Larry 
Published: December 2013

OBJECTIVES:
To understand mandated behavioral health (BH) screening in Massachusetts Medicaid including characteristics of screened children, predictors of positive screens, and whether screening identifies children without a previous BH history.

METHODS:
Massachusetts mandated BH screening in particularly among underidentified groups. 2008. Providers used a billing code and modifier to indicate a completed screen and whether a BH need was identified. Using MassHealth claims data, children with ≥300 days of eligibility in fiscal year (FY) 2009 were identified and categorized into groups based on first use of the modifier, screening code, or claim. Bivariate analyses were conducted to determine differences among groups. BH history was examined by limiting the sample to those continuously enrolled in FY 2008 and 2009. Multivariate logistic regression was used to determine predictors of positive screens.

RESULTS:
Of 355 490 eligible children, 46% had evidence of screening. Of those with modifiers, 12% were positive. Among continuously enrolled children (FY 2008 and FY 2009) with evidence of screening, 43% with positive modifiers had no BH history. This “newly identified” group were more likely to be female, younger, minority, and from rural residences (P &lt; .0001). Among children with modifiers; gender (male), age (5–7), being in foster care, recent BH history, and Hispanic ethnicity predicted having a positive modifier.

CONCLUSIONS:
The high rate of newly identified Medicaid children with a BH need suggests that screening is performing well, particularly among underidentified groups. To better assess screening value, future work on cost-effectiveness and the impact on subsequent mental health treatment is needed.
Fuel Formation and Conversion During In-Situ Combustion of Crude Oil
Authors: Hascakir Berna , Ross Cynthia M. , Castanier Louis M. , Kovscek Anthony R. 
Published: December 2013

Summary
In-situ combustion (ISC) is a successful method with great potential for thermal enhanced oil recovery. Field applications of ISC are limited, however, because the process is complex and not well-understood. A significant open question for ISC is the formation of coke or "fuel" in correct quantities that is sufficiently reactive to sustain combustion. We study ISC from a laboratory perspective in 1 m long combustion tubes that allow the monitoring of the progress of the combustion front by use of X-ray computed tomography (CT) and temperature profiles. Two crude oils—12°API (986 kg/m3) and 9°API (1007 kg/m3)—are studied. Cross-sectional images of oil movement and banking in situ are obtained through the appropriate analysis of the spatially and temporally varying CT numbers. Combustion-tube runs are quenched before front breakthrough at the production end, thereby permitting a post-mortem analysis of combustion products and, in particular, the fuel (coke and coke-like residues) just downstream of the combustion front. Fuel is analyzed with both scanning electron microscopy (SEM) and X-ray photoelectron spectroscopy (XPS). XPS and SEM results are used to identify the shape, texture, and elemental composition of fuel in the X-ray CT images. The SEM and XPS results aid efforts to differentiate among combustion-tube results with significant and negligible amounts of clay minerals. Initial results indicate that clays increase the surface area of fuel deposits formed, and this aids combustion. In addition, comparisons are made of coke-like residues formed during experiments under an inert nitrogen atmosphere and from in-situ combustion. Study results contribute to an improved mechanistic understanding of ISC, fuel formation, and the role of mineral substrates in either aiding or impeding combustion. CT imaging permits inference of the width and movement of the fuel zone in situ.
Association Between Glycemic Control and Morning Blood Pressure Surge With Vascular Endothelial Dysfunction in Type 2 Diabetic Patients
Authors: Yoda Koichiro , Inaba Masaaki , Hamamoto Kae , Yoda Maki , Tsuda Akihiro , Mori Katsuhito , Yamada Shinsuke , Emoto Masanori , Koyama Hidenori , Imanishi Yasuo 
Published: December 2013

OBJECTIVE
Morning blood pressure surge (MBPS) is an independent predictor of cardiovascular events. However, little is known about the association between glycemic control and MBPS, and its effect on vascular injury in patients with type 2 diabetes mellitus (T2DM). The current study examined the association between glycemic control and MBPS, and the involvement of MBPS in the development of vascular dysfunction in T2DM patients.

RESEARCH DESIGN AND METHODS
We examined MBPS in T2DM patients (25 male patients/25 female patients; mean age, 60.1 ± 13.2 years; n = 50) using 24-h ambulatory blood pressure monitoring, and assessed vascular function by brachial artery flow-mediated dilation (FMD) and nitroglycerin-mediated dilation (NMD).

RESULTS
HbA1c (ρ = 0.373, P = 0.009) and triglyceride (TG) (ρ = 0.375, P = 0.009) levels correlated significantly and positively with MBPS. In multiple regression analysis, including TG and HbA1c levels in addition to age and 24-h systolic blood pressure (SBP) as independent variables, HbA1c (β = 0.328, P = 0.016) and TG (β = 0.358, P = 0.014) were associated significantly in a positive manner with MBPS. In a noninsulin user, when homeostasis model assessment ratio (HOMA-R) was included in place of TG, HOMA-R emerged as a significant factor. MBPS (ρ = −0.289, P = 0.043) and HbA1c (ρ = −0.301, P = 0.035) correlated significantly and negatively with FMD, whereas 24-h SBP correlated with both FMD (ρ = −0.359, P = 0.012) and NMD (ρ = −0.478, P = 0.004). In multiple regression analysis, including age, gender, 24-h SBP, MBPS, LDL cholesterol, and HbA1c, MBPS (β = −0.284, P = 0.044) alone associated significantly in a negative manner with FMD, but not with NMD.

CONCLUSIONS
The current study demonstrated that poor glycemic control and insulin resistance are independently associated with the occurrence of MBPS in T2DM patients, which might be significantly associated with endothelial dysfunction.
Effects of Different Surface Treatments on Bond Strength Between Resin Cements and Zirconia Ceramics
Authors: Erdem A , Akar GC , Erdem A , Kose T 
Published: December 2013

SUMMARY
This study compares the bond strength of resin cement and yttrium-stabilized tetragonal zirconia polycrystalline (Y-TZP) ceramic with different surface conditioning methods. Two hundred presintered Y-TZP ceramic specimens were prepared, sintered (4 × 4 × 4 mm), and randomly assigned to four equal groups as control (C, no conditioning); airborne particle abraded (APA, air abrasion with 11 μm Al2O3); tribochemical silica coating/silane coupling system (TSC, Rocatec, air abrasion with 110 μm Al2O3, 30 μm silica-coated Al2O3 and silane); and laser (L, Er:YAG laser irradiation treated at a power setting of 200 mJ). After specimen preparation, composite resin cylinders were prepared and cemented with resin cements (Clearfil Esthetic, Panavia F 2.0, Rely X-U100, Super Bond C&B, and Multilink Automix) on the ceramic surfaces and kept in an incubator at 37°C for 60 days. All specimens were tested for shear bond strength with a universal testing machine, and fractured surfaces were evaluated by environmental scanning electron microscopy. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-tests (α=0.05). The bond strengths for C and L groups were not significantly different according to adhesive resin cement. APA and TSC resulted in increased bond strength for Panavia F 2.0 and Rely X-U100 resin cements. Additionally, TSC presented higher bond strength with Multilink Automix. Adhesive fracture between the ceramic and resin cement was the most common failure. Complete cohesive fracture at the ceramic or composite cylinders was not observed. Regardless of the adhesive resin cement used, laser treatment did not improve resin bond strength.
Co-occurrence of potentially preventable factors in 256 dog bite–related fatalities in the United States (2000–2009)
Authors: Patronek Gary J. , Sacks Jeffrey J. , Delise Karen M. , Cleary Donald V. , Marder Amy R. 
Published: December 2013

Abstract
Objective—To examine potentially preventable factors in human dog bite–related fatalities (DBRFs) on the basis of data from sources that were more complete, verifiable, and accurate than media reports used in previous studies.
Design—Prospective case series.
Sample—256 DBRFs occurring in the United States from 2000 to 2009.
Procedures—DBRFs were identified from media reports and detailed histories were compiled on the basis of reports from homicide detectives, animal control reports, and interviews with investigators for coding and descriptive analysis.
Results—Major co-occurrent factors for the 256 DBRFs included absence of an able-bodied person to intervene (n = 223 [87.1%]), incidental or no familiar relationship of victims with dogs (218 [85.2%]), owner failure to neuter dogs (216 [84.4%]), compromised ability of victims to interact appropriately with dogs (198 [77.4%]), dogs kept isolated from regular positive human interactions versus family dogs (195 [76.2%]), owners’ prior mismanagement of dogs (96 [37.5%]), and owners’ history of abuse or neglect of dogs (54 [21.1%]). Four or more of these factors co-occurred in 206 (80.5%) deaths. For 401 dogs described in various media accounts, reported breed differed for 124 (30.9%); for 346 dogs with both media and animal control breed reports, breed differed for 139 (40.2%). Valid breed determination was possible for only 45 (17.6%) DBRFs; 20 breeds, including 2 known mixes, were identified.
Conclusions and Clinical Relevance—Most DBRFs were characterized by coincident, preventable factors; breed was not one of these. Study results supported previous recommendations for multifactorial approaches, instead of single-factor solutions such as breed-specific legislation, for dog bite prevention.
Idiopathic ischemic necrosis of an accessory carpal bone in a dog
Authors: Harris Karen P. , Langley-Hobbs Sorrel J. 
Published: December 2013

Abstract
Case Description—A 6-year-old neutered female mixed-breed dog was evaluated because of a 6-week history of left forelimb lameness that varied in severity.
Clinical Findings—Radiography revealed expansile and lytic changes of the left accessory carpal bone (ACB). Results of histologic evaluation of ACB core biopsy specimens indicated areas of bone necrosis. The entire left ACB was excised and submitted for histologic evaluation; results confirmed a diagnosis of idiopathic ischemic necrosis.
Treatment and Outcome—Left pancarpal arthrodesis was performed to treat carpal hyperextension and persistent lameness. The dog had an excellent functional outcome with no other problems related to the carpus until its death 4 years later, further decreasing suspicion that the problem was attributable to an undetected neoplasm or bacterial or fungal osteomyelitis.
Clinical Relevance—The radiographic and histologic findings for the dog of this report were similar to previously reported findings for dogs with ischemic femoral head necrosis and humans with ischemic carpal (pisiform or lunate bone) necrosis. The etiology of the ischemic ACB necrosis in this dog was not determined. To the authors’ knowledge, this is the first report of a dog with idiopathic ischemic ACB necrosis. Idiopathic ischemic necrosis should be included as a differential diagnosis for dogs with lameness and destructive and expansile ACB radiographic lesions. An excellent functional outcome may be attained by means of ACB excision and pancarpal arthrodesis.
Corneal sequestrum in a dog with chronic unilateral keratoconjunctivitis sicca
Authors: Dubin Alexis J. , Pizzirani Stefano , Beamer Gillian L. 
Published: December 2013

Abstract
Case Description—A 14-year-old 8.2-kg (18.04-lb) castrated male Cairn Terrier with chronic keratoconjunctivitis sicca in the left eye was evaluated because of severe blepharospasm and a black plaque of 3 weeks’ duration.
Clinical Findings—Abnormalities of the left eye included a decreased Schirmer tear test value and the presence of a brownish-black plaque in the center of the cornea. The plaque was surrounded by fibrovascular tissue except at the medial aspect where there was mild malacia of the adjacent corneal stroma.
Treatment and Outcome—The plaque was removed by superficial keratectomy, and a conjunctival graft was performed. Histologic evaluation of the plaque and surrounding cornea revealed ulceration, stromal necrosis, and chronic suppurative keratitis with fibrosis and neovascularization. Evaluation of plaque sections that were stained with Gram and Von-Kossa stains yielded negative results for bacteria and mineralization, respectively; examination of sections stained with periodic acid–Schiff stain revealed multiple intracytoplasmic inclusions in macrophages. Virus isolation and a PCR assay for canine herpesvirus yielded negative results. Transmission electron microscopy revealed collagen disruption with interspersed macrophages and apoptotic keratocytes; no viral particles or evidence of other infectious agents was observed. The graft healed without complication and was trimmed 2 weeks after surgery. Four months after surgery, the Schirmer tear test value remained decreased from reference limits despite topical tacrolimus treatment, and pigmentary keratopathy was present surrounding the graft.
Clinical Relevance—Corneal sequestra are rare in species other than cats. In this dog, it was possible that chronic keratoconjunctivitis sicca might have contributed to the development of the corneal sequestrum.
Tenofovir or Zidovudine in Second-Line Antiretroviral Therapy after Stavudine Failure in Southern Africa
Authors: Wandeler Gilles , Gerber Florian , Rohr Julia , Chi Benjamin H , Orrell Catherine , Chimbetete Cleophas , Prozesky Hans , Boulle Andrew , Hoffmann Christopher J , Gsponer Thomas , Fox Matthew P , Zwahlen Marcel , Egger Matthias ,   Africa IeDEA Southern
Published: December 2013

Background There is debate over using tenofovir or zidovudine alongside lamivudine in second-line anti-retroviral therapy (ART) following stavudine failure. We analysed outcomes in cohorts from South Africa, Zambia and Zimbabwe Methods Patients aged ≥16 years who switched from a first-line regimen including stavudine to a ritonavir-boosted lopinavir-based second-line regimen with lamivudine or emtricitabine and zidovudine or tenofovir in seven ART programmes in southern Africa were included. We estimated the causal effect of receiving tenofovir or zidovudine on mortality and virological failure using Cox proportional hazards marginal structural models. Its parameters were estimated using inverse probability of treatment weights. Baseline characteristics were age, sex, calendar year and country. CD4+ T-cell count, creatinine and haemoglobin levels were included as time-dependent confounders. Results A total of 1,256 patients on second-line ART, including 958 on tenofovir, were analysed. Patients on tenofovir were more likely to have switched to second-line ART in recent years, spent more time on first-line ART (33 versus 24 months) and had lower CD4+ T-cell counts (172 versus 341 cells/ml) at initiation of second-line ART. The adjusted hazard ratio comparing tenofovir with zidovudine was 1.00 (95% CI 0.59, 1.68) for virological failure and 1.40 (0.57, 3.41) for death. Conclusions We did not find any difference in treatment outcomes between patients on tenofovir or zidovudine; however, the precision of our estimates was limited. There is an urgent need for randomized trials to inform second-line ART strategies in resource-limited settings.
Presence of Anti-Interferon Antibodies is Not Associated with Non-Response to Pegylated Interferon Treatment in Chronic Hepatitis B
Authors: Arends Pauline , van der Eijk Annemiek A , Sonneveld Milan J , Hansen Bettina E , Janssen Harry LA , Haagmans Bart L 
Published: December 2013

Background Several factors have been related to response to pegylated interferon (PEG-IFN) in chronic hepatitis B (CHB). The occurrence of anti-IFN antibodies is associated with non-response to PEG-IFN in chronic hepatitis C. This study investigated the association between anti-IFN antibodies and response to PEG-IFN in CHB. Methods Presence of anti-IFN antibodies was assessed at baseline and at 3 and 6 months post-treatment in 323 CHB patients treated with PEG-IFN for 1 year. Results At baseline, anti-IFN antibodies were detected in 112 (35%) patients. Prevalence was higher in HBeAg-negative compared to HBeAg-positive CHB (43% versus 31%, respectively; P=0.03). Detection of anti-IFN antibodies was not associated with age, sex or HBV genotype. Presence of anti-IFN antibodies at baseline was associated with previous IFN therapy failure ( P=0.04), which remained after adjustment for HBeAg status (OR 2.0, 95% CI 1.1, 3.7; P=0.03). Presence of anti-IFN antibodies at baseline was not associated with response, nor with HBV DNA or HBsAg decline (all P-values >0.3). Overall, 56 of 211 (27%) patients without anti-IFN at baseline developed anti-IFN antibodies after PEG-IFN treatment. Response rates did not differ between patients who developed anti-IFN antibodies and patients who did not develop anti-IFN antibodies during treatment ( P=0.1). Conclusions Anti-IFN antibodies may frequently be detected in CHB patients, and presence is associated with previous IFN therapy. However, presence or development of anti-IFN antibodies after PEG-IFN therapy is not associated with non-response to PEG-IFN treatment in CHB. There appears to be no future role for anti-IFN antibodies in predicting response to PEG-IFN in CHB.
Inhibition of HBV replication in vivo using helper-dependent adenovirus vectors to deliver antiviral RNA interference expression cassettes
Authors: Crowther Carol , Mowa Mohube B , Ely Abdullah , Arbuthnot Patrick B 
Published: December 2013

Background HBV is hyperendemic to southern Africa and parts of Asia, but licensed antivirals have little effect on limiting life-threatening complications of the infection. Although RNA interference (RNAi)-based gene silencing has shown therapeutic potential, difficulties with delivery of anti-HBV RNAi effectors remain an obstacle to their clinical use. To address concerns about the transient nature of transgene expression and toxicity resulting from immunostimulation by recombinant adenovirus vectors (Ads), utility of RNAi-activating anti-HBV helper-dependent (HD) Ads were assessed in this study. Methods Following intravenous administration of 5x109 unmodified or pegylated HD Ad infectious particles to HBV transgenic mice, HBV viral loads and serum HBV surface antigen levels were monitored for 12 weeks. Immunostimulation of HD Ads was assessed by measuring inflammatory cytokines, hepatic function and immune response to the co-delivered LacZ reporter gene. Results Unmodified and pegylated HD Ads transduced 80–90% of hepatocytes and expressed short hairpin RNAs (shRNAs) were processed to generate intended HBV-targeting guides. Markers of HBV replication were decreased by approximately 95% and silencing was sustained for 8 weeks. Unmodified HD Ads induced release of proinflammatory cytokines and there was evidence of an adaptive immune response to β-galactosidase. However the HD Ad-induced innate immune response was minimal in preparations that were enriched with infectious particles. Conclusions HD Ads have potential utility for delivery of therapeutic HBV-silencing sequences and alterations of these vectors to attenuate their immune responses may further improve their efficacy.
A Probabilistic Approach for the Generation of Learning Sessions Tailored to the Learning Styles of Learners
Authors: El Bouhdidi Jaber , Ghailani Mohamed , Fennan Abdelhadi 
Published: December 2013

In this paper we present an audacious solution based on Bayesian networks and educational approach for the construction of evolutionary personalized learning paths. We mean by evolutionary learning paths, paths that are composed gradually as learners advance in their learning, i.e in real time. To do this, the system selects the hypermedia units of learning to apprehend based on the results of formative assessments, psychological and cognitive characteristics of learner.
The architecture that we propose is based, firstly, on the semantic web, First, in order to model the domain model and to index learning resources so as to maximize their reuse, and then to represent the personal and cognitive traits of learners in a learner model while integrating their learning styles according to the Felder and Silverman model; and secondly, a probabilistic approach based on Bayesian networks that calculates the probability of success of each candidate hypermedia unit, for selecting those who are most appropriate for the construction of evolutionary personalized learning paths.
The proposed Bayesian model is validated with real data collected from an experimental study with a specimen of students.
Moduli Spaces of Vector Bundles over a Real Curve: ℤ/2-Betti Numbers
Authors: Baird Thomas 
Published: December 2013

AbstractModuli spaces of real bundles over a real curve arise naturally as Lagrangian submanifolds of the moduli space of semi–stable bundles over a complex curve. In this paper, we adapt the methods of Atiyah–Bott's “Yang–Mills over a Riemann Surface” to compute ℤ/2–Betti numbers of these spaces.