bluuebunny/crossref_metadata_2025_split huggingface dataset:
Depth Characteristics for the Electronic Futures Limit Order Book
Authors: Aidov Alexandre , Daigler Robert T. 
Published: January 2015

AbstractPrior literature provides limited information on the depth characteristics of futures markets, especially for U.S. exchanges, due to the lack of historical depth data from floor trading. A proprietary database remedies this problem by providing a five‐deep electronic futures limit order book. Our results show a large amount of depth beyond the best level, as well as more concentrated activity during the day than the night. We document symmetry between the bid and ask sides of the book, although no evidence of equality between the depth levels exists. Most important is that our results often contradict equity depth research. Overall, the structure of the limit order book helps us to understand the depth allocation by active traders in futures markets. © 2015 Wiley Periodicals, Inc. Jrl Fut Mark 35:542–560, 2015
Sphingomonas sp. is a Novel Cell Culture Contaminant
Authors: Asghar Muhammad Tahir , Al‐Ghanim K , Mahboob Shahid , Sharif Muhammad , Nazir Jawad , Shakoori Abdul Rauf 
Published: January 2015

ABSTRACTA novel contaminant was isolated from Madin Darby Bovine Kidney (MDBK) cells. The organism was unable to grow on standard microbiological media by conventional techniques, but grew well in Dulbecco's Modified Eagle's Medium (DMEM) containing high glucose concentration. The organism formed a white biofilm on the bottom without any signs of turbidity. Upon genome sequence analysis of 16 S rDNA, the contaminant was identified as Sphingomonas sp. Shah, a member of the group α‐Proteobacteria. Neutral red dye uptake method confirmed clear cytotoxic potential of the bacterium on A‐549 cells. The organism was capable of invading and infecting different mammalian cell lines: MDBK, ZZ‐R, 293‐T, A549, and HeLa cells. Infected cells showed a variety of cytopathic effects including vacuolation at perinuclear area, cytoplasmic granulation and membrane blebbing. Microscopic analysis of the infected cells revealed the presence of cytoplasmic vacuoles harboring motile organisms. Apparently local serum preparations seem to be the source of this contamination, which is imperceptibly passed on from one culture passage to the other and ultimately leading to serious cytopathic manifestations. J. Cell. Biochem. 116: 934–942, 2015. © 2015 Wiley Periodicals, Inc.
MicroRNA‐124 Regulates Neuronal Differentiation of Mesenchymal Stem Cells by Targeting Sp1 mRNA
Authors: Mondanizadeh Mahdieh , Arefian Ehsan , Mosayebi Ghasem , Saidijam Massoud , Khansarinejad Behzad , Hashemi Seyed Mahmoud 
Published: January 2015

ABSTRACTMicroRNAs play an important role in neuronal development and function. miR‐124 is the most abundantly expressed miRNA in the nervous system. Several different mRNA targets have been proposed for miR‐124, but the precise function of endogenous miR‐124 and its mRNA targets remain to be further elucidated. Specificity protein 1 (Sp1) is a transcription factor that plays key roles in many cell processes including cell cycle. However, this transcription factor is nearly absent in differentiated neurons and a remarkable suppression of Sp1 expression was shown after neurogenesis. Since miR‐124 is expressed abundantly in neurons and because Sp1 levels decrease during neurogenesis, it is possible that miR‐124 could regulate the expression of Sp1 during neuronal development. Therefore, the aim of the present study was to evaluate the putative targeting of Sp1 by miR‐124. Overexpression of miR‐124 using a plasmid coding for pri‐miR‐124 in HEK293 cells decreased the expression of Sp1 mRNA. The results of dual‐luciferase reporter assay demonstrated that miR‐124 directly targeted the 3′‐untranslated regions of Sp1 mRNA. To evaluate whether Sp1 expression was regulated by miR‐124 during the process of neuronal differentiation, Adipose‐derived mesenchymal stem cells (A‐MSCs) were differentiated into neuron‐like cells. The results of qPCR analysis showed that with the gradual increase of miR‐124 expression during neurogenesis, the expression of Sp1 mRNA decreased accordingly. In summary, this study demonstrated for the first time that miR‐124 is able to suppress Sp1 expression, which in turn affected the neuronal differentiation of mesenchymal stem cells. J. Cell. Biochem. 116: 943–953, 2015. © 2015 Wiley Periodicals, Inc.
Down‐regulated SOX4 Expression Suppresses Cell Proliferation, Metastasis and Induces Apoptosis in Xuanwei Female Lung Cancer Patients
Authors: Zhou Yongchun , Wang Xicai , Huang Yunchao , Chen Yan , Zhao Guangqiang , Yao Qian , Jin Congguo , Huang Youguang , Liu Xin , Li Guangjian 
Published: January 2015

ABSTRACTThe transcription factor SOX4 has functional importance in foetal lung maturation and tumorigenesis in a number of cancers. However, its biological functions in the progression of lung tumorigenesis remain unclear. In this study, we found that the expression levels of SOX4 mRNA and protein were significantly higher in Xuanwei female lung cancer tissues than in benign lung lesions. The patients with high expression of the SOX4 protein had a higher pathological grade, lymph node (LN) metastasis, poor tumor differentiation and worse prognosis than those patients with low expression of SOX4. Knockdown of the SOX4 gene in the Xuanwei female lung cancer cell line XWLC‐05 resulted in apoptotic morphological changes, decreased cell proliferation, invasion and migration. Furthermore, knockdown of the SOX4 gene resulted in obvious sub‐G1 peaks and induction of apoptosis through upregulation of caspase‐3 expression, while in cells treated with a caspase‐3 inhibitor, apoptosis induced by silencing SOX4 expression was inhibited. In vivo analysis in nude mice further confirmed that knockdown of SOX4 suppressed tumor growth. In conclusion, SOX4 appears to be an important tumor suppressor gene in the regulation of Xuanwei female lung cancer cell proliferation, apoptosis and metastases, and it may be a potential target for effective lung cancer therapy. J. Cell. Biochem. 116: 1007–1018, 2015. © 2015 Wiley Periodicals, Inc.
Three dimensional mesoscale analysis of translamellar cross‐bridge morphologies in the annulus fibrosus using optical coherence tomography
Authors: Han Sang Kuy , Chen Chao‐Wei , Wierwille Jerry , Chen Yu , Hsieh Adam H. 
Published: January 2015

ABSTRACTThe defining characteristic of the annulus fibrosus (AF) of the intervertebral disc (IVD) has long been the lamellar structures that consist of highly ordered collagen fibers arranged in alternating oblique angles from one layer to the next. However, a series of recent histologic studies have demonstrated that AF lamellae contain elastin‐ and type VI collagen‐rich secondary “cross‐bridge” structures across lamellae. In this study, we use optical coherence tomography (OCT) to elucidate the three‐dimensional (3‐D) morphologies of these translamellar cross‐bridges in AF tissues. Mesoscale volumetric images by OCT revealed a 3‐D network of heterogeneously distributed cross‐bridges. The results of this study confirm the translamellar cross‐bridge is identifiable as a distinguishable structure, which lies in the interbundle space of adjacent lamellae and crisscrosses multiple lamellae in the radial direction. In contrast to previously proposed models extrapolated from 2–D sections, results from this current study show that translamellar cross‐bridges exist as a complex, interconnected network. We also found much greater variation in lengths of cross‐bridges within the interbundle space of lamellae (0.8–1.4 mm from the current study versus 0.3–0.6 mm from 2–D sections). OCT‐based 3–D morphology of translamellar cross‐bridge provides novel insight into the AF structure. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:???–???, 2015.
Novel fetal and maternal sonographic findings in confirmed cases of Beckwith–Wiedemann syndrome
Authors: Kagan Karl Oliver , Berg Christoph , Dufke Andreas , Geipel Annegret , Hoopmann Markus , Abele Harald 
Published: January 2015

AbstractObjectiveThe objective of the study was to examine the prenatal anomalies in fetuses with Beckwith–Wiedemann syndrome (BWS).MethodsThe study included a retrospective assessment of 12 pregnancies that were seen at three tertiary referral centres (Universities of Tübingen, Bonn, and Cologne/Germany). The genetic mutation, the results of the second trimester ultrasound examination, and the outcome of the pregnancies are shown. Biometric data were transformed into z‐values.ResultsMedian gestational age at the time of examination was 22.6 (range 19.0–29.7) weeks of gestation. In all cases, the head circumference (HC) and the femur length (FL) were within the normal range, but the HC‐FL ratio was above the 95th centile in 75% of the cases. An exomphalos, macroglossia, and visceromegaly were observed in 67%, 50%, and 83% of the cases, and in 58% and 83%, there were polyhydramnios and placentamegaly respectively. The fetal pancreas was identified in three quarters of the cases. A third of the women had large, overstimulation‐like ovaries, although each pregnancy was conceived naturally. In four cases, beta‐human chorionic gonadotropin (hCG) levels were measured and mean hCG levels were 498 106 IU/L.DiscussionBesides exomphalos, BWS should be considered if there is macroglossia, a distinct growth pattern, pancreatic hyperplasia, placentamegaly, and substantially increased levels of beta‐hCG. © 2015 John Wiley & Sons, Ltd.
Female protection from slow‐pressor effects of angiotensin II involves prevention of ROS production independent of NMDA receptor trafficking in hypothalamic neurons expressing angiotensin 1A receptors
Authors: Marques‐Lopes Jose , Lynch Mary‐Katherine , Van Kempen Tracey A. , Waters Elizabeth M. , Wang Gang , Iadecola Costantino , Pickel Virginia M. , Milner Teresa A. 
Published: January 2015

ABSTRACTRenin–angiotensin system overactivity, upregulation of postsynaptic NMDA receptor function, and increased reactive oxygen species (ROS) production in the hypothalamic paraventricular nucleus (PVN) are hallmarks of angiotensin II (AngII)‐induced hypertension, which is far more common in young males than in young females. We hypothesize that the sex differences in hypertension are related to differential AngII‐induced changes in postsynaptic trafficking of the essential NMDA receptor GluN1 subunit and ROS production in PVN cells expressing angiotensin Type 1a receptor (AT1aR). We tested this hypothesis using slow‐pressor (14‐day) infusion of AngII (600 ng/kg/min) in mice, which elicits hypertension in males but not in young females. Two‐month‐old male and female transgenic mice expressing enhanced green fluorescent protein (EGFP) in AT1aR‐containing cells were used. In males, but not in females, AngII increased blood pressure and ROS production in AT1aR–EGFP PVN cells at baseline and following NMDA treatment. Electron microscopy showed that AngII increased cytoplasmic and total GluN1–silver‐intensified immunogold (SIG) densities and induced a trend toward an increase in near plasmalemmal GluN1–SIG density in AT1aR–EGFP dendrites of males and females. Moreover, AngII decreased dendritic area and diameter in males, but increased dendritic area of small (<1 µm) dendrites and decreased diameter of large (>1 µm) dendrites in females. Fluorescence microscopy revealed that AT1aR and estrogen receptor β do not colocalize, suggesting that if estrogen is involved, its effect is indirect. These data suggest that the sexual dimorphism in AngII‐induced hypertension is associated with sex differences in ROS production in AT1aR‐containing PVN cells but not with postsynaptic NMDA receptor trafficking. Synapse 69:148–165, 2015.  © 2015 Wiley Periodicals, Inc.
Carbon-limited fed-batch production of medium-chain-length polyhydroxyalkanoates by aphaZ-knockout strain ofPseudomonas putidaKT2440
Authors: Vo Minh Tri , Ko Kenton , Ramsay Bruce 
Published: January 2015

AbstractA medium-chain-length poly-3-hydroxyalkanote (MCL-PHA) depolymerase knockout mutant of Pseudomonas putida KT2440 was produced by double homologous recombination. A carbon-limited shake-flask study confirmed that depolymerase activity was eliminated. Lysis of both mutant and wild-type strains occurred under these conditions. In carbon-limited, fed-batch culture, the yield of unsaturated monomers from unsaturated substrate averaged only 0.62 mol mol−1 for the phaZ minus strain compared to 0.72 mol mol−1 for the wild type. The mutant strain also produced more CO2 and less residual biomass from the same amount of carbon substrate. However, most results indicated that elimination of PHA depolymerase activity had little impact on the overall yield of biomass and PHA.
Synthesis of New Salt‐Resistant Sulfonate Gemini Surfactants with Hydroxyl Groups
Authors: Zhou Ming , Xia Liangliang , He Yinglan , Zhang Ling , Qiao Xin , Zhong Xiang 
Published: January 2015

AbstractThough the gemini surfactants have unique properties, their high cost limits their scope of application. The new salt‐resistant sulfonate gemini surfactants disodium 5,12‐bis(alkyloxymethyl)‐4,7,10,13‐tetraoxa‐2,15‐dihydroxyhexadecane 1,16‐disulfonates bearing two alkyl chains, two hydroxyl and two sulfonate groups were prepared from fatty alcohol, epichlorohydrin, glycol, sodium hydroxide, sodium bisulfite and sodium sulfite. Their synthesis exhibited the advantages of low raw material cost, mild reaction conditions and easy operation. The structures of prepared compounds were confirmed by FTIR and 1H NMR. These gemini surfactants show excellent surface‐active properties, good salt resistance and excellent foaming ability.
Development of Functional Polyurethane–ZnO Hybrid Nanocomposite Coatings from Thevetia peruviana Seed Oil
Authors: Siyanbola T. O. , Sasidhar K. , Rao B. V. S. K. , Narayan Ramanuj , Olaofe O. , Akintayo E. T. , Raju K. V. S. N. 
Published: January 2015

AbstractThe present article reports eco‐friendly multi‐functional polyurethane–ZnO hybrid nanocomposite coatings obtained from Thevetia peruviana seed oil (TPSO). Initially, the polyols were prepared by treating TPSO with glycerol and the formation was supported by Fourier transform infrared (FT‐IR) and 1H‐NMR studies. In the next stage, siloxane functionalized ZnO nanoparticles were added to the polyol mixture in different weight percentages (0, 1 and 2 %) and then treated with excess 4,4′‐diisocyanatodicyclohexylmethane (H12MDI) in order to synthesize isocyanate terminated polyurethane nanocomposites. The polyurethane hybrids were then casted as thin films and cured under atmospheric moisture. After complete curing they were characterized by using FT‐IR, 1H‐NMR, 13C‐NMR, X‐ray diffraction, scanning electron microscopy, thermogravimetric analysis, and dynamic mechanical thermal analysis techniques. The hybrid nanocomposites showed superior thermo‐mechanical and anti‐corrosive properties compared to pristine polyurethane. Also, due to the presence of nano ZnO in the polyurethane matrix, the composite coatings are showing excellent resistance towards various bacterial and fungal stains.
Flattening simulations of 3D thick sheets made of fiber composite materials
Authors: Morioka Kotaro , Ohtake Yutaka , Suzuki Hiromasa , Nagai Yukie , Hishida Hiroyuki , Inagaki Koichi , Nakamura Takeshi , Watanabe Fumiaki 
Published: January 2015

Abstract
Recently, fiber composite materials have been attracting attention from industry because of their remarkable material characteristics, including light weight and high stiffness. However, the costs of products composed of fiber materials remain high because of the lack of effective manufacturing and designing technologies. To improve the relevant design technology, this paper proposes a novel simulation method for deforming fiber materials. Specifically, given a 3D model with constant thickness and known fiber orientation, the proposed method simulates the deformation of a model made of thick fiber-material. The method separates a 3D sheet model into two surfaces and then flattens these surfaces into two dimensional planes by a parameterization method with involves cross vector fields. The cross vector fields are generated by propagating the given fiber orientations specified at several important points on the 3D model. Integration of the cross vector fields gives parameterization with low-stretch and low-distortion.
Intake patterns and dietary associations of soya protein consumption in adults and children in the Canadian Community Health Survey, Cycle 2.2
Authors: Mudryj Adriana N. , Aukema Harold M. , Yu Nancy 
Published: January 2015

Soya foods are one of the recommended alternatives to meat in many dietary guidelines. While this is expected to increase the intake of some nutrients, potential concerns regarding others have been raised. The purpose of the present study was to examine the prevalence and the association of soya food consumption with nutrient intakes and dietary patterns of Canadians (age ≥ 2 years). Cross-sectional data from the 2004 Canadian Community Health Survey (Cycle 2.2;n33 218) were used to classify soya consumers and non-consumers. Soya consumers were further divided into two groups based on their soya protein intake. Sample weights were applied and logistic regression analysis was used to explore the association between nutrient intakes and soya consumption, with cultural background, sex, age and economic status being included as covariates. On any given day, 3·3 % (n1085) of Canadians consume soya foods, with females, Asian Canadians and adults with post-secondary education being more likely to be soya consumers. As a whole, adolescent and adult respondents who had consumed at least one soya food during their 24 h dietary recall had higher energy intakes, as well as increased intakes of nutrients such as protein, fibre, vitamin C, vitamin B6, naturally occurring folate, thiamin, Ca, P, Mg, PUFA, Fe and K and lowered intakes of saturated fat. These data indicate that soya food consumption is associated with improved diet quality of Canadians. However, future research is necessary to investigate the association between increased energy intake and soya consumption.
The Dark Side of Empathy: Mimesis, Deception, and the Magic of Alterity
Authors: Bubandt Nils , Willerslev Rane 
Published: January 2015

AbstractThis article challenges the tendency, both academic and popular, to assign empathy the status of a virtue. The widespread inclination to associate empathy with the morally and socially “good”—with compassion, understanding, cultural bonding, and non-violent sociality—ignores what we propose to call the “dark side of empathy”: that is, the multiple ways in which empathy is routinely deployed to manipulate, seduce, deceive, and dehumanize others by means of vicariousness. Two diverse ethnographic cases, of hunting in Siberia and political violence in Indonesia, provide the empirical background for a discussion of the complex relationship of empathy to mimesis, deception, violence, and sociality.
Toward a Critical Hauntology: Bare Afterlife and the Ghosts of Ba Chúc
Authors: Lincoln Martha , Lincoln Bruce 
Published: January 2015

AbstractWhile cross-disciplinary analysis of ghosts and haunting has burgeoned in recent decades, much of this scholarship presumes the figure of the ghost as a less than literal apparition. We propose that writers such as Jacques Derrida and Avery Gordon, who make use of the ghostly as a trope, are in fact describing a phenomenon we term secondary haunting, distinct from accounts of unquiet spirits who address the living directly with specific demands for redress: a visceral and often frightening experience we term primary haunting. Drawing on a contemporary account of the ghosts of a massacre in a Vietnamese village, we explore the complex interaction of primary and secondary haunting, the different kinds of memory work they engage in and the different moral communities they mobilize.
Subaltern Bureaucrats and Postcolonial Rule: Indigenous Professional Registers of Engagement with the Chilean State
Authors: Radcliffe Sarah A. , Webb Andrew J. 
Published: January 2015

AbstractWhat is the experience of a racial subaltern on becoming an employee of a postcolonial state? Latin America has undertaken widespread multicultural state reform, often in response to pressure from nation-wide social movements and transnational human rights activism. This provides us with a window into ways in which subaltern individuals negotiate their place in a historically exclusionary state with norms of whiteness, European codes, and literal and metaphoric distance from marginal populations. Previous research has emphasized the cooptation of subaltern actors by neoliberal postcolonial states, but we argue that a close reading of subaltern accounts yields important insights into their experiences of ambivalence, ambiguity, and agency. Neoliberal state restructuring entrained a parallel, and in many cases interconnected process that generated ambivalence among civil servants. We draw on interviews with state employees associated with multicultural educational reforms in Chile to document the registers through which indigenous subalterns position themselves regarding the politics of interculturalism and the costs of serving the state.
Fast photodynamics of azobenzene probed by scanning excited-state potential energy surfaces using slow spectroscopy
Authors: Tan Eric M. M. , Amirjalayer Saeed , Smolarek Szymon , Vdovin Alexander , Zerbetto Francesco , Buma Wybren Jan 
Published: January 2015

AbstractAzobenzene, a versatile and polymorphic molecule, has been extensively and successfully used for photoswitching applications. The debate over its photoisomerization mechanism leveraged on the computational scrutiny with ever-increasing levels of theory. However, the most resolved absorption spectrum for the transition to S1(nπ*) has not followed the computational advances and is more than half a century old. Here, using jet-cooled molecular beam and multiphoton ionization techniques we report the first high-resolution spectra of S1(nπ*) and S2(ππ*). The photophysical characterization reveals directly the structural changes upon excitation and the timescales of dynamical processes. For S1(nπ*), we find that changes in the hybridization of the nitrogen atoms are the driving force that triggers isomerization. In combination with quantum chemical calculations we conclude that photoisomerization occurs along an inversion-assisted torsional pathway with a barrier of ~2 kcal mol−1. This methodology can be extended to photoresponsive molecular systems so far deemed non-accessible to high-resolution spectroscopy.
Centromeric binding and activity of Protein Phosphatase 4
Authors: Lipinszki Zoltan , Lefevre Stephane , Savoian Matthew S. , Singleton Martin R. , Glover David M. , Przewloka Marcin R. 
Published: January 2015

AbstractThe cell division cycle requires tight coupling between protein phosphorylation and dephosphorylation. However, understanding the cell cycle roles of multimeric protein phosphatases has been limited by the lack of knowledge of how their diverse regulatory subunits target highly conserved catalytic subunits to their sites of action. Phosphoprotein phosphatase 4 (PP4) has been recently shown to participate in the regulation of cell cycle progression. We now find that the EVH1 domain of the regulatory subunit 3 of Drosophila PP4, Falafel (Flfl), directly interacts with the centromeric protein C (CENP-C). Unlike other EVH1 domains that interact with proline-rich ligands, the crystal structure of the Flfl amino-terminal EVH1 domain bound to a CENP-C peptide reveals a new target-recognition mode for the phosphatase subunit. We also show that binding of Flfl to CENP-C is required to bring PP4 activity to centromeres to maintain CENP-C and attached core kinetochore proteins at chromosomes during mitosis.