bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of joint disease in mucopolysaccharidosis type I mice
Authors: de Oliveira Patricia G. , Baldo Guilherme , Mayer Fabiana Q. , Martinelli Barbara , Meurer Luise , Giugliani Roberto , Matte Ursula , Xavier Ricardo M. 
Published: June 2013

SummaryMucopolysaccharidoses (MPS) are lysosomal storage disorders characterized by mutations in enzymes that degrade glycosaminoglycans (GAGs). Joint disease is present in most forms of MPS, including MPS I. This work aimed to describe the joint disease progression in the murine model of MPS I. Normal (wild‐type) and MPS I mice were sacrificed at different time points (from 2 to 12 months). The knee joints were collected, and haematoxylin–eosin staining was used to evaluate the articular architecture. Safranin‐O and Sirius Red staining was used to analyse the proteoglycan and collagen content. Additionally, we analysed the expression of the matrix‐degrading metalloproteinases (MMPs), MMP‐2 and MMP‐9, using immunohistochemistry. We observed progressive joint alterations from 6 months, including the presence of synovial inflammatory infiltrate, the destruction and thickening of the cartilage extracellular matrix, as well as proteoglycan and collagen depletion. Furthermore, we observed an increase in the expression of MMP‐2 and MMP‐9, which could conceivably explain the degenerative changes. Our results suggest that the joint disease in MPS I mice may be caused by a degenerative process due to increase in proteases expression, leading to loss of collagen and proteoglycans. These results may guide the development of ancillary therapies for joint disease in MPS I.
Conceptualization and measurement of personal norms regarding meal preparation
Authors: Costa Ana Isabel de Almeida 
Published: June 2013

AbstractPast research has revealed that individual beliefs about the proper amount of time, effort and care that should be put into preparing daily household meals can lead to a moral obligation to cook regularly. Such perceived sense of duty may affect not only the frequency with which consumers prepare meals from scratch but also resort to using different home meal replacements. This paper presents the conceptualization, development and validation of psychographic scales that measure the direction and strength of personal norms regarding meal preparation. Literature review, qualitative research and pre‐testing generated a pool of 40 items related to the constructs of interest. These were subsequently administered to a random sample of 590 adults as part of a household mail survey on meals and meal preparation. Exploratory factor analysis and reliability testing of survey results produced four new psychometric measures with adequate construct validity:Personal norm about cooking,Awareness of the psychological consequences of ready meal use, Awareness of the health consequences of ready meal useandAscription of responsibility for cooking. These measures can be used to segment consumers according to their personal norms about meal preparation. This will help nutrition educators and policy makers to target messages about healthy eating more effectively, and it may also provide valuable support to the product development and marketing activities of food manufacturers.
Histone deacetylase inhibitors activate CIITA and MHC class II antigen expression in diffuse large B‐cell lymphoma
Authors: Cycon Kelly A. , Mulvaney Kathleen , Rimsza Lisa M. , Persky Daniel , Murphy Shawn P. 
Published: June 2013

SummaryDiffuse large B‐cell lymphoma (DLBCL), the most common form of non‐Hodgkin's lymphoma (NHL) diagnosed in the USA, consists of at least two distinct subtypes: germinal centre B (GCB) and activated B‐cell (ABC). Decreased MHC class II (MHCII) expression on the tumours in both DLBCL subtypes directly correlates with significant decreases in patient survival. One common mechanism accounting for MHCII down‐regulation in DLBCL is reduced expression of the MHC class II transactivator (CIITA), the master regulator of MHCII transcription. Furthermore, reduced CIITA expression in ABC DLBCL correlates with the presence of the transcriptional repressor positive regulatory domain‐I‐binding factor‐1 (PRDI‐BF1). However, the mechanisms underlying down‐regulation of CIITA in GCB DLBCL are currently unclear. In this study, we demonstrate that neither PRDI‐BF1 nor CpG hypermethylation at the CIITA promoters are responsible for decreased CIITA in GCB DLBCL. In contrast, histone modifications associated with an open chromatin conformation and active transcription were significantly lower at the CIITA promoters in CIITA− GCB cells compared with CIITA+ B cells, which suggests that epigenetic mechanisms contribute to repression of CIITA transcription. Treatment of CIITA− or CIITAlow GCB cells with several different histone deacetylase inhibitors (HDACi) activated modest CIITA and MHCII expression. However, CIITA and MHCII levels were significantly higher in these cells after exposure to the HDAC‐1‐specific inhibitor MS‐275. These results suggest that CIITA transcription is repressed in GCB DLBCL cells through epigenetic mechanisms involving HDACs, and that HDACi treatment can alleviate repression. These observations may have important implications for patient therapy.
Abdominal wall reconstruction with Two‐step Technique (TST): a prospective study in 20 patients
Authors: Al Zarouni Marwan , Trelles Mario A. , Leclère Franck M. 
Published: June 2013

AbstractAbdominal wall defects continue to be a challenging problem for reconstructive surgeons. The aim of our study was to report a 3‐year experience using a simple Two‐step Technique (TST) to treat abdominal wall defects. Between January 2008 and December 2010, 20 patients with abdominal wall defects were treated by TST. Patients had a mean age of 37·5 ± 14·9 years (range: 22–85 years); 5 were women and 15 were men. The size of the defects was prospectively analysed. Early and late complications were recorded. Hospital stay, post‐procedure downtime and patient overall satisfaction were systematically assessed. A secondary defect resulting from self‐manipulation and an infection were responsible for a complication rate of 10%. Both underwent successful surgical revision which led to full resolution. The average hospital stay was 11·2 ± 4·9 weeks for the series. Long‐term complications were scar hyperpigmentation in 11 cases, scar hypertrophy in 5 cases and scar widening in 3 cases. Mean patient satisfaction was 8·3 ± 0·5 [visual analogue scale (VAS) 0–10]. Average downtime post surgery was 4·1 ± 1·2 weeks. The mean follow‐up was 24·6 ± 6·7 months. Reconstruction of abdominal wall defect with the TST is a reliable and reproducible technique. This technique provides excellent outcomes, and we anticipate that it will become widespread in the near future.
Wells syndrome and its relationship to Churg–Strauss syndrome
Authors: Ratzinger Gudrun , Zankl Julia , Zelger Bernhard 
Published: June 2013

AbstractBackground  Wells syndrome has been described as an inflammatory disorder based on typical clinical appearance combined with the histopathological presence of eosinophilic infiltrates and flame figures in the absence of vasculitis. Churg–Strauss syndrome, on the other hand, is primarily a diffuse, necrotizing vasculitis but is also typically displaying eosinophils and flame figures. Despite several parallels, the present understanding of these two diseases excludes any pathogenetic relationship.Methods  We describe the clinical course and histopathological appearance of three patients who had initially been diagnosed with Wells syndrome that developed into Churg–Strauss syndrome during the course of their disease.Results  The clinical presentation of all three patients led to the diagnosis of Wells syndrome by independent specialists. Histopathology showed an eosinophilic infiltrate and flame figures next to features of leukocytoclastic vasculitis. Detailed examination revealed asthma bronchiale and additional symptoms indicating Churg–Strauss syndrome. The initial diagnosis of Wells syndrome had to be revised to Churg–Strauss syndrome.Conclusion  We conclude that Wells syndrome could be the starting point of a pathogenetic process that might reach its maximum in Churg–Strauss syndrome. As a clinical consequence, patients with Wells syndrome should be evaluated and followed for Churg–Strauss syndrome.
Punch Biopsy of the Skin: Effect of Temperature and Local Anesthesia with Ethyl Chloride Freezing
Authors: SERUP JØRGEN 
Published: June 2013

Abstract. The effect of local anesthesia with ethyl chloride freezing on biopsy size and quality for microscopy was studied postmortem. Fourmm punch biopsies were taken from the abdominal skin by different techniques: manual punch and drill (250 RPM) at 8°C and 33 to 36°C, ethyl chloride, and injection of lidocaine for local anesthesia. Histologic sections were assessed, and defatted dried weight (DW) was determined as an expression of biopsy size.After ethyl chloride freezing, microscopy showed more trauma (p < 0.001) and characteristic, marginal tissue‐breaks (p < 0.01). More biopsies (p < 0.05) were not proper for microscopy. DW and the variances showed no differences.It is concluded that local anesthesia with ethyl chloride freezing results in tissue trauma and inferior quality of the tissue specimens for microscopy.
A Multivariate Analysis of Factors Affecting Wound‐Healing Time
Authors: ROBINS PERRY , DAY CALVIN L. , LEW ROBERT A. 
Published: June 2013

Abstract For post‐Mohs surgical defects of the head and neck, the width of the defect is the best predictor of the length of that time it will take the wound to heal. This conclusion is based on a multivariate analysis, testing the effect of the following factors on wound‐healing time: (1) age, (2) sex, (3) sound location, (4) wound width, (5) wound length, and (6) wound length × wound width.After accounting for wound width, none of the other factors significantly influenced the rate of wound healing. The 64 wounds evaluated were dressed daily with one of five bandages.When compared with the controls, and after correcting for wound width, each of the test bandages shortened wound‐healing time. Among the treatment groups, no significant differences were found in the cultures or in the appearance of the wounds.
Article: Cardiovascular Effects of Topical 50% Trichloroacetic Acid and Baker's Phenol Solution
Authors: STAGNONE GREGORY J. , ORGEL MICHAEL G. , STAGNONE JAMES J. 
Published: June 2013

Abstract. Phenol and trichloroacetic acid (TCA) are used for cosmetic face peeling. Deaths associated with phenol have been ascribed to the cardiotoxicity. Our purpose has been to study and compare the cardiovascular responses to these two agents in a rat model.Fifteen rats underwent abdominal epilation. Two to six days later a surface area comparable to the human face (16 cm2) was delineated on the abdomen. Baker's phenol or 50% TCA was applied to this area. Cardiac rate and rhythm and arterial pressure were monitored. Once stable, the same agent was applied to the entire abdomen for further study.TCA has been shown to be a safer agent than phenol in this rat model. Assuming that this model simulates the human response, monitoring would appear to be indicated during full‐face Baker's phenol peeling.
Use of the Carbon Dioxide Laser in Dermatologic Surgery
Authors: OLBRICHT SUZANNE M. 
Published: June 2013

background. The effective and safe use of the carbon dioxide (CO2) laser in the 1990s has been facilitated by technologic advances and widespread clinical use producing a better understanding of laser‐tissue interactions, refinements in technique, and modifications of the list of indications. Safety guidelines have evolved from identification of complications.objective. To discuss the basic principles and applications of CO2 laser surgery.methods. Types of CO2 lasers and their applications are discussed.conclusion. Today, use of the carbon dioxide laser simplifies many procedures, is reasonably safe, is effective for its intended usages, and is the preferred treatment of several cutaneous disorders.
Solitary Mastocytoma in an Adult
Authors: ASHINOFF ROBIN , SOTER NICHOLAS A. , FREEDBERG IRWIN M. 
Published: June 2013

BACKGROUND. In approximately 65% of patients, mastocytosis presents between birth and 15 years of age. Although solitary mastocytomas usually appear within the first 3 months of life, in unusual circumstances they may appear in adulthood.OBJECTIVE. The rare entity of solitary mastocytoma in adulthood and the simple treatment method of excision are discussed.METHODS. Simple surgical excision without manipulation of the lesion was performed.RESULTS. The lesion was surgically excised without recurrence.CONCLUSION. Solitary mastocytoma is a rare lesion in adulthood. The differential diagnosis includes a melanocytic nevus, xanthogranuloma and leukemia cutis. Surgical excision offers a rapid, relatively simple and effective mode of treatment.
Influence of self‐esteem, locus of control, and organizational climate on psychological empowerment in a sample of Chinese teachers
Authors: Wang Jin‐Liang , Zhang Da‐Jun , Jackson Linda A. 
Published: June 2013

AbstractThis study examined the effects of locus of control, self‐esteem, and organizational climate on psychological empowerment in a sample of Chinese teachers. Participants were 1,272 Chinese teachers (460 men and 821 women) residing in six provinces in China. Results indicated that self‐esteem was a positive predictor of psychological empowerment. Several dimensions of organizational climate, namely professional communication, decision‐making, appraisal and recognition, supportive leadership, and professional growth, were also positive predictors of teachers’ psychological empowerment. Locus of control, although related to psychological empowerment, had no predictive effects when other factors related to empowerment were considered.
Evolutionary history influences the effects of water–energy dynamics on oak diversity in Asia
Authors: Xu Xiaoting , Wang Zhiheng , Rahbek Carsten , Lessard Jean‐Philippe , Fang Jingyun 
Published: June 2013

AbstractAimWater–energy dynamics are often correlated with geographical patterns of terrestrial plant richness. However, the relative importance of water and energy on species richness is still being debated. Some studies suggest a transition in the relative importance of water and energy along a latitudinal gradient, i.e. that water is the most important factor at low latitudes, whereas energy is the leading factor at high latitudes. The generality of this transition is yet to be established and is the focus of the current study. In particular, we examine whether differences in the evolutionary histories of two subgenera of Asian oaks influence the derived models of water–energy dynamics.LocationAsia (70–140° E; 10–60° N).MethodsWe performed geographically weighted regressions to quantify geographical variation in the relative importance of water and energy on the species richness of Asian oaks (Quercus L.). We also evaluated how evolutionary history influences the relative importance of water and energy in determining species richness, by comparing patterns in the two oak subgenera: Quercus subg. Quercus and Quercus subg. Cyclobalanopsis. In particular, we assessed how the ancestral climatic niche of these two subgenera (i.e. subgenus Quercus of temperate origin versus subgenus Cyclobalanopsis of tropical origin) relates to the relative importance of water and energy on contemporary diversity patterns.ResultsWe found no geographical transition line in the relative importance of water and energy along the latitudinal gradient studied. Instead, we found that the importance of energy relative to water on the species richness of subgenus Quercus increased from mid‐latitudes (warm temperate regions) towards both tropical and boreal regions: this pattern might reflect that this subgenus originated in the temperate region. In contrast, the importance of both water and energy on the species richness of the tropical subgenus Cyclobalanopsis increased from tropical to boreal regions, probably due to a lack of adaptation to winter coldness.Main conclusionsOur results suggest that differences in the evolutionary history – specifically the ancestral climatic niche – of the two subgenera of oaks influence the effects of water–energy dynamics on species richness along a latitudinal gradient through niche conservatism.
Range size patterns of New World oscine passerines (Aves): insights from differences among migratory and sedentary clades
Authors: Morales‐Castilla Ignacio , Rodríguez Miguel Á. , Kaur Ramnik , Hawkins Bradford A. 
Published: June 2013

AbstractAimTo quantify the contributions of environment, phylogeny and geography to variation in the breeding and non‐breeding geographical range sizes of oscine passerines.LocationWestern Hemisphere.MethodsBreeding range sizes were estimated for 420 species, and non‐breeding ranges were estimated for 122 migratory species. Phylogenetic, environmental and geographical (spatial) eigenvectors were used to partition cross‐species variation in range size. The strengths of environmental and phylogenetic signals were quantified and compared among all species, and between migratory and sedentary oscines.ResultsPhylogenetic, environmental and geographical structure explained most of the variation in range size, accounting for 95% of the variation in breeding range sizes of migratory birds. The three components overlapped extensively, with most variation explained by differences in environmental niches. Models for breeding ranges of migratory species contained the strongest phylogenetic, environmental and geographical signals at the species level. In contrast, models for non‐breeding ranges of migratory species contained the weakest phylogenetic and environmental signals (5.7% and 65.2% of variance explained, respectively). The phylogenetic signal was consistently stronger for migratory breeding ranges than for the other groups.Main conclusionsOscine range sizes contain a low to moderate phylogenetic signal that overlaps with environmental and geographical associations. The significance of phylogenetic signal suggests that the evolution of range size is not entirely labile, which is probably a result of the non‐labile evolution of associated traits. Environmental, geographical and phylogenetic variables can account for most of the variance in species‐level range size, with qualitatively similar patterns for migratory and sedentary species. Nonetheless, the stronger environmental and phylogenetic signals in the breeding ranges of migratory species may reflect both that migration is a phylogenetically conserved trait and that the subset of species able to breed in ‘recently’ deglaciated regions is more severely constrained by macroclimatic filtering.
Yersiniosis in Atlantic cod, Gadus morhua (L.), characterization of the infective strain and host reactions
Authors: Gudmundsdottir B K , Gudmundsdottir S , Gudmundsdottir S , Magnadottir B 
Published: June 2013

AbstractA disease outbreak in farmed Atlantic cod caused by Yersinia ruckeri is reported. Mortality started following vaccination of cod reared in two tanks (A and B). The accumulated mortality reached 1.9% in A and 4.8% in B in the following 30 days when treatment with oxytetracycline was applied. Biochemical and molecular analysis of Y. ruckeri isolates from the cod and other fish species from fresh and marine waters in Iceland revealed a high salinity‐tolerant subgroup of Y. ruckeri serotype O1. Infected fish showed clinical signs comparable with those of Y. ruckeri ‐infected salmonids, with the exception of granuloma formations in infected cod tissues, which is a known response of cod to bacterial infections. Immunohistological examination showed Y. ruckeri antigens in the core of granulomas and the involvement of immune parameters that indicates a strong association between complement and lysozyme killing of bacteria. Experimental infection of cod with a cod isolate induced disease, and the calculated LD50 was 1.7 × 104 CFU per fish. The results suggest that yersiniosis can be spread between populations of freshwater and marine fish. Treatment of infected cod with antibiotic did not eliminate the infection, which can be explained by the immune response of cod producing prolonged granulomatous infection.
Dopaminergic neurotoxicity ofS‐ethylN,N‐dipropylthiocarbamate (EPTC), molinate, andS‐methyl‐N,N‐diethylthiocarbamate (MeDETC) inCaenorhabditis elegans
Authors: Caito Samuel W. , Valentine William M. , Aschner Michael 
Published: June 2013

AbstractEpidemiological studies corroborate a correlation between pesticide use and Parkinson's disease (PD). Thiocarbamate and dithiocarbamate pesticides are widely used and produce neurotoxicity in the peripheral nervous system. Recent evidence from rodent studies suggests that these compounds also cause dopaminergic (DAergic) dysfunction and altered protein processing, two hallmarks ofPD. However,DAergic neurotoxicity has yet to be documented. We assessedDAergic dysfunction inCaenorhabditis elegans(C. elegans) to investigate the ability of thiocarbamate pesticides to induceDAergic neurodegeneration. Acute treatment with eitherS‐ethylN,N‐dipropylthiocarbamate (EPTC), molinate, or a common reactive intermediate of dithiocarbamate and thiocarbamate metabolism,S‐methyl‐N,N‐diethylthiocarbamate (MeDETC), to gradual loss ofDAergic cell morphology and structure over the course of 6 days in worms expressing green fluorescent protein (GFP) under aDAergic cell specific promoter.HPLCanalysis revealed decreasedDAcontent in the worms immediately following exposure to MeDETC,EPTC, and molinate. In addition, worms treated with the three test compounds showed a drastic loss ofDAergic‐dependent behavior over a time course similar to changes inDAergic cell morphology. Alterations in theDAergic system were specific, as loss of cell structure and neurotransmitter content was not observed in cholinergic, glutamatergic, orGABAergic systems. Overall, our data suggest that thiocarbamate pesticides promote neurodegeneration andDAergic cell dysfunction inC. elegans, and may be an environmental risk factor forPD.
Age‐dependent alterations of the kynurenine pathway in the YAC128 mouse model of Huntington disease
Authors: Mazarei Gelareh , Budac David P. , Lu Ge , Adomat Hans , Tomlinson Guns Emma S. , Möller Thomas , Leavitt Blair R. 
Published: June 2013

AbstractIndoleamine 2,3 dioxygenase (Ido1), the first and rate‐limiting enzyme of the kynurenine pathway (KP), is a striatally enriched gene with increased expression levels in the YAC128 mouse model of Huntington disease (HD). Our objective in this study was to delineate age‐related KP alterations in this model. Three enzymes potentially catalyze the first step of the KP; Ido1 and Indoleamine 2,3 dioxygenase‐2 were highly expressed in the striatum and Tryptophan 2,3 dioxygenase (Tdo2) in the cerebellum. During development, Ido1 mRNA expression is dynamically regulated and chronically up‐regulated in YAC128 mice. Kynurenine (Kyn) to tryptophan (Trp) ratio, a measure of activity in the first step of the KP, was elevated in YAC128 striatum, but no change in Tdo2 mRNA levels or Kyn to Trp ratio was detected in the cerebellum. Ido1 induction was coincident with Trp depletion at 3 months and Kyn accumulation at 12 months of age in striatum. Changes in downstream KP metabolites of YAC128 mice generally followed a biphasic pattern with neurotoxic metabolites reduced at 3 months and increased at 12 months of age. Striatally specific induction of Ido1 and downstream KP alterations suggest involvement in HD pathogenesis, and should be taken into account in future therapeutic developments for HD.