bluuebunny/crossref_metadata_2025_split huggingface dataset:
A variability study of regional alveolar oxygen tension measurement in humans using hyperpolarized 3He MRI
Authors: Hamedani Hooman , Kadlecek Stephen J. , Ishii Masaru , Emami Kiarash , Kuzma Nicholas N. , Xin Yi , Rossman Milton , Rizi Rahim R. 
Published: February 2013

PurposeA systematic study of the short‐term and long‐term variability of regional alveolar partial pressure of oxygen tension (pAO2) measurements using 3He magnetic resonance imaging was presented. Additionally, the repeatability of the average evaluated pAO2 was compared with that of the standard pulmonary function tests.MethodsPulmonary function test and pAO2 imaging were performed on 4 nonsmokers (1 M, 3 F, 56 ± 1.7 years) and 4 smokers (3 M, 1 F, 52 ± 7.5 years) during three visits over the course of 2 weeks. Two measurements were performed per visit. Variability of pAO2 was assessed using a mixed‐effect model, with an intraclass correlation coefficient calculated for each group. The coefficient of variation of pAO2 over the 3‐day period was also compared with the coefficient of variation of pulmonary function test results.ResultsShort‐term regional variability based on intraclass correlation coefficient was 0.71 for nonsmokers, and 0.63 for smokers, with long‐term variability significantly lower at 0.59 and 0.47, respectively. While the coefficient of variation of the average pAO2 was similar to the repeatability of the diffusing capacity of CO, it was significantly higher than that of Forced Vital Capacity (P = 0.02).ConclusionShort‐term and long‐term pAO2 variability differences were used as an indication of true physiological changes in order to measure technical reproducibility. Smokers show higher physiologic variability and less technical reproducibility. The suggested pAO2‐imaging technique showed a reasonable regional repeatability in nonsmokers as well as the ability to detect differences between the two groups with similar reproducibility and superior discriminatory ability when compared with pulmonary function tests. Magn Reson Med 70:1557–1566, 2013. © 2013 Wiley Periodicals, Inc.
Dynamic imaging of the fetal heart using metric optimized gating
Authors: Roy Christopher W. , Seed Mike , van Amerom Joshua F. P. , Al Nafisi Bahiyah , Grosse‐Wortmann Lars , Yoo Shi‐Joon , Macgowan Christopher K. 
Published: February 2013

PurposeAdvances in fetal cardiovascular magnetic resonance imaging have been limited by the absence of a reliable cardiac gating signal. The purpose of this work was to develop and validate metric‐optimized gating (MOG) for cine imaging of the fetal heart.Theory and MethodsCine MR and electrocardiogram data were acquired in healthy adult volunteers for validation of the MOG method. Comparison of MOG and electrocardiogram reconstructions was performed based on the image quality for each method, and the difference between MOG and electrocardiogram trigger times. Fetal images were also acquired, their quality evaluated by experienced radiologists, and the theoretical error in the MOG trigger times were calculated.ResultsExcellent agreement between electrocardiogram and MOG reconstructions was observed. The experimental errors in adult MOG trigger times for all five volunteers were ± (7, 25, 17, 8, and 13) ms. Fetal images captured normal and diseased cardiac dynamics.ConclusionMOG for cine imaging of the fetal myocardium was developed and validated in adults. Using MOG, the first gated MR images of the human fetal myocardium were obtained. Small moving structures were visualized during radial contraction, thus capturing normal fetal cardiac wall motion and permitting assessment of cardiac function. Magn Reson Med 70:1598–1607, 2013. © 2013 Wiley Periodicals, Inc.
Subcutaneous panniculitis‐like T‐cell lymphoma in the pediatric age group: A lymphoma of low malignant potential
Authors: Huppmann Alison R. , Xi Liqiang , Raffeld Mark , Pittaluga Stefania , Jaffe Elaine S. 
Published: February 2013

AbstractBackgroundSubcutaneous panniculitis‐like T‐cell lymphoma (SPTCL) is a rare neoplasm of mature αβ cytotoxic T‐cells. Most commonly occurring in young adults, few reports are described in children. A separate analysis of a significant cohort of pediatric patients has not previously been performed.ProcedureWe analyzed the pathology including molecular results as well as available clinical data from 16 pediatric patients (age 5 months to 21 years) who had a total of 19 biopsies submitted to the National Cancer Institute from 1999 to 2011. This included 6 males and 10 females.ResultsMost patients (10/16, 62.5%) had multiple skin lesions at the time of biopsy. Histologic features included rimming of adipocytes by atypical lymphocytes, fat necrosis, and karyorrhectic debris. Four biopsies showed only partial involvement by lymphoma; and plasma cells were identified in 14/19 (74%) cases, including three in which they were focally prominent. The neoplastic cells in general were positive for CD3, CD8, TIA‐1, and βF1 and were negative for CD4 and CD56. CD5 expression was weak to negative in 5/8 cases (63%). A clonal T‐cell receptor gene rearrangement was demonstrated in 11/17 (65%). Patients were treated with a variety of agents. While 5/9 (56%) patients had evidence of recurrent skin lesions, no deaths were attributed to disease for the seven patients with follow‐up information.ConclusionsPediatric SPTCL shares many clinical and pathologic features with adult SPTCL. The presence of partial involvement or admixed plasma cells makes the differential diagnosis with reactive conditions challenging in some cases. Pediatr Blood Cancer 2013; 60: 1165–1170. © 2013 Wiley Periodicals, Inc.
Persistent defective membrane trafficking in epithelial cells of patients with familial hemophagocytic lymphohistiocytosis type 5 due to STXBP2/MUNC18‐2 mutations
Authors: Stepensky Polina , Bartram Jack , Barth Thomas F. , Lehmberg Kai , Walther Paul , Amann Kerstin , Philips Alan D. , Beringer Ortraud , Zur Stadt Udo , Schulz Ansgar , Amrolia Persis , Weintraub Michael , Debatin Klaus‐Michael , Hoenig Manfred , Posovszky Carsten 
Published: February 2013

AbstractBackgroundFamilial hemophagocytic lymphohistiocytosis (FHL) is a rare primary immune disorder defined by mutations in the syntaxin binding protein 2 (STXBP2) alias MUNC18‐2. Despite defective immunity and a hyper‐inflammatory state, clinical findings such as neurological, gastrointestinal, and bleeding disorders are present in a significant number of patients and suggest an impaired expression and function of STXBP2 in cells other than cytotoxic lymphocytes.ProcedureWe investigated four patients with FHL5 suffering from severe enteropathy and one of whom also had renal tubular dysfunction despite successful hematopoietic stem cell transplantation (HSCT). Gastrointestinal and renal biopsy specimens were analyzed by immunohistochemistry and electron microscopy.ResultsHistopathology revealed an intracytoplasmatic accumulation of PAS‐positive granules and an enlarged intracytoplasmatic CD10‐positive band along the apical pole of enterocytes. Electron microscopy revealed short microvilli and granules filled with electro lucent material. In addition, we described mildly dilated renal tubules and electron micrographs displayed a higher number of cytoplasmic inclusions, electrodense lysosomal and electrolucent endosomal vesicles.ConclusionMutations in STXBP2 do not only affect cytotoxic T lymphocytes but also cause changes in the intestinal and renal epithelium resulting in severe, osmotic diarrhea and renal proximal tubular dysfunction. These defects persist after successful treatment of hemophagocytic lymphohistocytosis by HSCT. Clinical manifestations in FHL5 patients despite successful HSCT may therefore be related to defective membrane trafficking in the gut and kidney. Pediatr Blood Cancer 2013; 60: 1215–1222. © 2013 Wiley Periodicals, Inc.
Effects of adenotonsillectomy on polysomnographic parameters in children with sickle cell disease
Authors: Finch Paul , Stocks Rose Mary , Smeltzer Matthew P. , Kimble Amy , Schoumacher Robert , Hankins Jane S. 
Published: February 2013

AbstractObstructive sleep apnea (OSA) in the pediatric sickle cell disease (SCD) population can promote nightly hemoglobin oxygen desaturation, which increases the risk of central nervous system insult and may impair cognitive function. Adenotonsillectomy can ameliorate OSA symptoms, but its effect in children with SCD has not been fully investigated. We reviewed the effects of adenotonsillectomy in thirteen children with SCD by comparison of pre and post‐adenotonsillectomy polysomnography (PSG) parameters. Significant reduction in hemoglobin oxygen desaturation, decreased apnea‐hypopnea index, and increased rapid eye movement sleep occurred after adenotonsillectomy. Adenotonsillectomy promotes improvement in sleep quality in children with SCD and PSG‐confirmed OSA. Pediatr Blood Cancer 2013; 60: E26–E28. © 2013 Wiley Periodicals, Inc.
Polymers with shape memory effect from renewable resources: crosslinking of polyesters based on isosorbide, itaconic acid and succinic acid
Authors: Goerz Oliver , Ritter Helmut 
Published: February 2013

AbstractPolycondensation of isosorbide with itaconic acid and succinic acid was performed in the presence of sulfuric acid as a catalyst in toluene around 140 °C under microwave irradiation. Molar ratios of itaconic acid were varied to investigate the influence of itaconic acid on molecular weights and glass transition temperatures. For polyesters of isosorbide, itaconic acid and succinic acid Tg values were found from 57 °C to 65 °C. The molecular weights obtained varied from 1200 Da for poly(isosorbide itaconate) UPE100 up to 3500 Da for poly(isosorbide succinate) UPE0. The copolyesters obtained were crosslinked radically with dimethyl itaconate giving a round shaped material with slightly raised Tg values up to 74 °C. Furthermore, with dimethyl itaconate crosslinked copolyesters showed a one‐way shape memory effect upon heating after deformation at the glass transition temperature. © 2013 Society of Chemical Industry
Serum fibrinogen alpha C‐chain 5.9 kDa fragment as a biomarker for early detection of hepatic fibrosis related to hepatitis C virus
Authors: Sogawa Kazuyuki , Noda Kenta , Umemura Hiroshi , Seimiya Masanori , Kuga Takahisa , Tomonaga Takeshi , Nishimura Motoi , Kanai Fumihiko , Imazeki Fumio , Takizawa Hirotaka , Yoneda Masato , Nakajima Atsushi , Tsutsumi Mikihiro , Yokosuka Osamu , Nomura Fumio 
Published: February 2013

PurposeClinical application of biomarker candidates discovered by proteomic analysis is challenging. The purpose of this study was to standardize preanalytical conditions for measurement of serum levels of fibrinogen alpha C‐chain 5.9 kDa fragment (FIC 5.9) and to test the diagnostic value of this peptide for detection of early hepatic fibrosis in patients with hepatitis C virus (HCV)‐related chronic hepatitis.Experimental designSerum FIC 5.9 levels were measured by a sandwich ELISA. Effects on the serum FIC 5.9 level of temperature, the time between venipuncture and serum separation, and the types of collection tubes used were examined. The diagnostic value of serum FIC 5.9 as an early indicator of hepatic fibrosis due to HCV was then assessed.ResultsFIC 5.9 was produced in a time‐ and temperature‐dependent manner after venipuncture. Abnormal FIC 5.9 values were found in 89.5% of FI stage patients. Receiver operating characteristic analyses confirmed the superiority of FIC 5.9 over other conventional markers for early detection of fibrosis.Conclusions and clinical relevanceThe serum FIC 5.9 level may be an early indicator of hepatic fibrosis in HCV‐related chronic liver diseases. This study provides an example of a pipeline from biomarker discovery by proteome analysis to assay optimization and preliminary clinical validation.
Proteomic patterns of colonic mucosal tissues delineate Crohn's colitis and ulcerative colitis
Authors: Seeley Erin H. , Washington Mary K. , Caprioli Richard M. , M'Koma Amosy E. 
Published: February 2013

PurposeAlthough Crohn's colitis (CC) and ulcerative colitis (UC) share several clinical features, they have different causes, mechanisms of tissue damage, and treatment options. Therefore, the accurate diagnosis is of paramount importance in terms of medical care. The distinction between CC/UC is made on the basis of clinical, radiologic, endoscopic, and pathologic interpretations but cannot be differentiated in up to 15% of inflammatory bowel disease patients. Correct management of this “indeterminate colitis” depends on the accuracy of future, and yet not known, destination diagnosis (CC/UC).Experimental designWe have developed a proteomic methodology that has the potential to discriminate between UC/CC. The histologic layers of 62 confirmed UC/CC tissues were analyzed using MALDI‐MS for proteomic profiling.ResultsA Support Vector Machine algorithm consisting of 25 peaks was able to differentiate spectra from CC and UC with 76.9% spectral accuracy when using a leave‐20%‐out cross‐validation. Application of the model to the entire dataset resulted in accurate classification of 19/26 CC patients and 36/36 UC patients when using a 2/3 correct cutoff. A total of 114 peaks were found to have Wilcoxin rank sum p‐values of less than 0.05.Conclusion and clinical relevanceThis information may provide new avenues for the development of novel personalized therapeutic targets.
Role of defects in enhancing room temperature ferromagnetism of Mn doped ZnO nanoparticles
Authors: Ekhande L. V. , Dhas V. V. , Kolekar Y. D. , Ghosh K. , Date S. K. , Patil S. I. 
Published: February 2013

AbstractThe soft chemical route was used in the synthesis of undoped and 5% Mn doped ZnO nanocrystalline powders. XRD, TEM, TGA/DTA, FTIR, and superconducting quantum interference device techniques were used to study the structural, nano/microstructural, thermal decomposition and metastability aspects as a function of calcination temperatures (400–1100 °C) and magnetic properties. The evolution of the major wurtzite phase (ZnO) and minor non‐stoichiometric nanocrystalline defect cubic spinel phase (ZnMnO3–δ) at various temperatures is clearly seen. The magnetic hysteresis loop is observed at room temperature in the undoped and doped samples calcined at 400 °C. Interestingly, the hysteresis loop parameters (Ms, Hc) are found to enhance dramatically as soon as the concentration of the minor phase is large enough up to the calcination temperature 700 °C. In contrast, the magnetic hysteresis loop vanishes slowly for the sample calcined at 1000 °C, it disappears completely. The room temperature ferromagnetic behavior at 400 °C is understood in terms of intrinsic cationic/anionic defects, extrinsic defects associated with the various species chemisorbed on the surface of the nanoparticles of undoped and Mn doped ZnO. During thermal annealing a nanocrysatllline seconadary phase of non‐stoichiometric defect cubic spinel ZnMnO3–δ is formed, contributing to the enhancement of ferromagnetic behavior. All our experimental results are discussed in terms of model comparing various structural and localized electronic defects formed in the nanocrystalline powder.
Lgr5-EGFP Marks Taste Bud Stem/Progenitor Cells in Posterior Tongue
Authors: Yee Karen K. , Li Yan , Redding Kevin M. , Iwatsuki Ken , Margolskee Robert F. , Jiang Peihua 
Published: February 2013

Abstract
Until recently, reliable markers for adult stem cells have been lacking for many regenerative mammalian tissues. Lgr5 (leucine-rich repeat-containing G-protein-coupled receptor 5) has been identified as a marker for adult stem cells in intestine, stomach, and hair follicle; Lgr5-expressing cells give rise to all types of cells in these tissues. Taste epithelium also regenerates constantly, yet the identity of adult taste stem cells remains elusive. In this study, we found that Lgr5 is strongly expressed in cells at the bottom of trench areas at the base of circumvallate (CV) and foliate taste papillae and weakly expressed in the basal area of taste buds and that Lgr5-expressing cells in posterior tongue are a subset of K14-positive epithelial cells. Lineage-tracing experiments using an inducible Cre knockin allele in combination with Rosa26-LacZ and Rosa26-tdTomato reporter strains showed that Lgr5-expressing cells gave rise to taste cells, perigemmal cells, along with self-renewing cells at the bottom of trench areas at the base of CV and foliate papillae. Moreover, using subtype-specific taste markers, we found that Lgr5-expressing cell progeny include all three major types of adult taste cells. Our results indicate that Lgr5 may mark adult taste stem or progenitor cells in the posterior portion of the tongue.
Innovative Leichtbau‐Fügetechnologien
Authors: Emrich Stefan , Kopnarski Michael 
Published: February 2013

AbstractAn Beispielen aus der Kleb‐ und Ultraschallschweißtechnik wird dargestellt, wie verschiedene Methoden der Vakuum gestützten Analytik im Bereich innovativer Leichtbau‐Fügetechnologien eingesetzt werden. Ziel dabei ist es, Fügemechanismen aufzuklären und somit Optimierungspotentiale für Festigkeit und Alterungsbeständigkeit von Verbunden zu erschließen. Die Flugzeit‐Sekundärionenmassenspektrometrie (ToF‐SIMS) wird zur Charakterisierung von Epoxidharzklebstoffen eingesetzt und liefert quantitative und lokale Informationen zur Zusammensetzung und Struktur der duroplastischen Kunststoffe. Hierbei erweisen sich multivariate Datenanalysemethoden als sehr hilfreich.Bei ultraschallgeschweißten Aluminiumverbunden liefern hoch ortsaufgelöste Analysemethoden Informationen zur Rekonstruktion der physikalischchemischen Prozesse und Bedingungen während des Fügevorgangs.Die Steigerung von Festigkeit und Langzeitbeständigkeit ultraschallbzw. induktionsgeschweißten Hybridverbunde aus Aluminium und carbonfaserverstärkten thermoplastischen Kunststoffen (CFK‐PA66) kann durch den Einsatz Vakuum gestützter Analyseverfahren auf eine intensive mikromechanische Verklammerung des Matrixpolymers mit der Aluminiumoxidschicht zurückgeführt werden.
Reinigung vor der Beschichtung
Authors: Tränkenschuh Barbara 
Published: February 2013

AbstractVor der Beschichtung wird die Oberfläche des zu beschichtenden Bauteils gereinigt. Dafür gibt es verschiedene Reinigungsanlagen und Chemikalien. Nach der Reinigung vor der Beschichtung muss die Oberfläche beschichtungsfähig sein.Es wird vorgestellt, welchen Einfluss die Bearbeitung des Bauteils auf seine Oberfläche hat. Die Effektivität der Reinigung vor der Beschichtung wird aufgezeigt und in den Kontext des gesamten Fertigungsprozesses gestellt. Die Unterteilung des Fertigungsprozesses sollte nicht nach Orten getrennt werden. Stattdessen sollten die Bearbeitungsschritte, die einen Einfluss auf die Beschaffenheit und Beschichtungsfähigkeit der Metalloberfläche haben, als eine Einheit betrachtet werden. All diese Schritte sind im Idealfall so aufeinander abgestimmt, dass die Oberfläche fehlerfrei beschichtet werden kann.
Optik braucht Vakuum
Authors: Rietmann Thomas , Gottschall Sophie , Sahib Christoffer , Flämmich Michael , Bergner Ute 
Published: February 2013

AbstractEine Vielzahl von Anwendungen in der Hochtechnologie verwenden optische Signale, optische Informationen oder Laserstrahlung im Vakuum. Diese Sinale müssen oftmals in ein Vakuum hinein oder aus einem Vakuum heraus überführt werden.Dieser Artikel stellt die Anforderungen und Eigenschaften für Bauteile dar, die ein optisches Element und ein Vakuumbauteil hermetisch dicht miteinander verbinden. Vier Verbindungsmethoden werden vorgestellt, mit denen es möglich ist, optische und vakuumtechnisch relevante Anforderungen zu erfüllen und diese insbesondere nach der Verbindung zu erhalten. Es werden Vor‐ und Nachteile dieser Verbindungsmethoden diskutiert und typische Eigenschaftsprofile abgeleitet.Aus diesen Betrachtungen ergibt sich, dass die Vakuumoptik letztlich für jede Anwendung die für die spezifischen Anforderungen angepasste Lösung bereithält.
Seawater circulation in sediments driven by interactions between seabed topography and fluid density
Authors: Konikow L. F. , Akhavan M. , Langevin C. D. , Michael H. A. , Sawyer A. H. 
Published: February 2013

Key PointsSeawater recirculation can be driven by seabed topography‐density interactionTopography‐density seawater circulation occurs under limited conditionsTopography‐density seawater circulation typically comprises 20‐30% of SGD
Colorectal Cancer and Crohn’s Colitis: Clinical Implications From 313 Surgical Patients
Authors: Scaringi Stefano , Di Martino Carmela , Zambonin Daniela , Fazi Marilena , Canonico Giuseppe , Leo Francesca , Ficari Ferdinando , Tonelli Francesco 
Published: February 2013

AbstractBackgroundThe relation between Crohn’s colitis (CC) and colorectal cancer is still controversial. Several case reports and retrospective studies have shown that patients with Crohn’s disease (CD) have a 6‐ to 20‐fold higher risk to develop CRC than does the normal population. The extent of disease (extensive colitis), presence of anal fistula, age > 40 years, strictures, and length of disease >10 years may be important determinants for increasing risk. Despite this evidence, other population‐based studies have shown no increased risk of colon or rectal cancer. The aim of this study was to investigate retrospectively factors that may predict the development of cancer.MethodsWe searched the histopathologic database of the Digestive Surgery Unit at Careggi University Hospital for CC patients (January 1987 to September 2011) and identified 313 patients with CC who underwent surgery.ResultsThere are 11 (3.5 %) of adenocarcinomas. Multivariate analysis showed disease duration (p = 0.001), age at CD diagnosis (p = 0.002), distal localization (p = 0.045), and penetrating disease (p = 0.041) to be risk factors. Multivariate analysis showed that 40 patients who had undergone previous immunosuppressive therapy had a significant risk of developing CRC (p = 0.026).ConclusionsCrohn’s colitis patients who require surgery are at higher risk for developing CRC, particularly those whose disease duration is >10 years, have distal localization, age at diagnosis was <40 years, and have penetrating disease. Previous immunosuppressive therapy should be better investigated. We recommend surgery for any patient presenting with colonic strictures.
Quantitative imaging of directional transport through plasmodesmata in moss protonemata via single-cell photoconversion of Dendra2
Authors: Kitagawa Munenori , Fujita Tomomichi 
Published: February 2013

Abstract
Cell-to-cell transport of molecules in plants must be properly regulated for plant growth and development. One specialized mechanism that plants have evolved involves transport through plasmodesmata (PD), but when and how transport of molecules via PD is regulated among individual cells remains largely unknown, particularly at the single-cell level. Here, we developed a tool for quantitatively analyzing cell-to-cell transport via PD at a single-cell level using protonemata of Physcomitrella patens and a photoconvertible fluorescent protein, Dendra2. In the filamentous protonemal tissues, one-dimensional intercellular communication can be observed easily. Using this system, we found that Dendra2 was directionally transported toward the apex of the growing protonemata. However, this directional transport could be eliminated by incubation in the dark or treatment with a metabolic inhibitor. Thus, we propose that directional transport of macromolecules can occur via PD in moss protonemata, and may be affected by the photosynthetic and metabolic activity of cells.