bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men
Authors: Eid Hossam A , Taha Tarek H , Alahmari Manea M , Alqarni Abdullah Awn S , Alshehri Abdulkarim Ali H 
Published: November 2013

ABSTRACTRecent advances in DNA technology have revolutionized forensic identification procedures. Teeth dentin and pulp are rich sources of DNA material, which can be successfully extracted and it provides us with valuable information on individuals, systemic health including fertility status.Aim of studyThe aim of this study was to use DNA material extracted from human teeth pulp for detection of fertility status of men.Materials and methodsTwenty extracted premolar teeth of systemic disease free male Saudi individuals (45 years average age) were collected; eight of them were infertile while others were fertile and were used as control group. This information was concealed until the PCR analysis was performed. The results of recorded patient information was matched with the results of the DNA analysis.ResultsResults showed that the gene (sY83) an important gene of AZFa region in Y chromosome is important for male fertility. It was later evident that the infertile patients suffered from azoospermia, and that information is completely matched with our results.ConclusionUsing DNA extracted from dental pulp can be used successfully in determining fertility status of human which may help in an accurate personal identification specially in extreme circumstances.How to cite this articleEid HA, Taha TH, Alahmari MM, Alqarni AAS, Alshehri AAH. Utilization of Dental Pulp DNA as Diagnostic Molecular Marker for Fertility Detection in Men. J Contemp Dent Pract 2013;14(4):590-594.
Evaluation of Propofol as a General Anesthetic Agent for Minor Oral Surgical Procedure
Authors: Kalra Amit , Medsinge Sonali V , Jaggi Nitin , Baghla Gunpreet Kaur , Purohit Nikhil 
Published: November 2013

ABSTRACT
Nausea and vomiting following anesthesia is a distressing problem for the patient as it increases the recovery time, intensity of nursing care and delays discharge. The aim of randomized controlled single blind study is to evaluate the efficacy and safety of subhypnotic doses of propofol for the prevention of postoperative nausea and vomiting (PONV) in day care management of cases in oral and maxillofacial surgeries. Twenty-five patient of ASA-1 with age ranging from 12 to 40 years were scheduled for various maxillofacial surgical cases like fracture, cyst enucleation, surgical removal of 3rd molar, etc. were given propofol at the dose of 2 to 2.5 mg/kg as induction dose and sedation was maintained with the dose 5 to 10 mg/min. There was no significant effect on heart rate, systolic and diastolic blood pressure, respiratory rate and oxygen saturation intraoperatively. In conclusion, a subhypnotic dose of propofol is fast acting, safe and easily controllable, short acting general anesthetic agent with rapid recovery. The study found that the PONV was significantly reduced in the patient with propofol, no hemodynamic derangements were noted in the postoperative period.

How to cite this article
Jaggi N, Kalra A, Baghla GK, Medsinge SV, Purohit N. Evaluation of Propofol as a General Anesthetic Agent for Minor Oral Surgical Procedure. J Contemp Dent Pract 2013;14(4):605-609
Evaluation of the Relationship between Bruxism and Premature Occlusal Contacts
Authors: Safari Anahita , Jowkar Zahra , Farzin Mitra 
Published: November 2013

ABSTRACTAimThis study evaluates the relationship between occlusal interferences and premature contacts and bruxism by determining the relationship between unassisted and assisted nonworking interferences.Materials and methodsIn this study, 60 subjects (14 males and 46 females) that consisted of 30 bruxers (7 males, 23 females) and 30 nonbruxers (7 males, 23 females) were selected after completion of a questionnaire based on the exclusion criteria. Occlusal interferences in the centric relation and eccentric movements in the two groups were evaluated and recorded. Data were analyzed by SPSS software (version 16) using the Chi-square and paired t-tests.ResultsThe results showed a statistically significant relationship between bruxism and nonworking interferences (p < 0.05). There was no statistically significant relationship in the centric relation and other eccentric movements (p > 0.05). The number of assisted nonworking occlusal contacts was more than unassisted nonworking occlusal contacts.ConclusionAccording to the results of this study, there is a relationship between certain types of occlusal interferences (nonworking interferences) and bruxism. Hence, it would be useful to examine occlusal contacts in bruxing patients to eliminate probable causative or contributing occlusal factors. Both assisted and unassisted nonworking occlusal contacts should be evaluated.Clinical implicationBruxism is an oral habit that consists of involuntary rhythmic or spasmodic nonfunctional gnashing, grinding or teeth clenching, other than chewing movements by the mandible. Bruxism may lead to occlusal trauma, tooth wear, fracture of the teeth and fillings, and hypertrophy of the masticatory muscles. Treatment of bruxism needs a correct diagnosis. Therefore, it is useful to determine the relationship between occlusal interferences and bruxism in order to prevent its development by adjusting for these interferences.How to cite this articleSafari A, Jowkar Z, Farzin M. Evaluation of the Relationship between Bruxism and Premature Occlusal Contacts. J Contemp Dent Pract 2013;14(4):616-621.
Gingival Microleakage of Class V Composite Restorations with Fiber Inserts
Authors: Ahmed Walaa , El-Badrawy Wafa , Kulkarni Gajanan , Prakki Anuradha , El-Mowafy Omar 
Published: November 2013

ABSTRACT

Aim
This study investigated the effect of different fiber inserts (glass and polyethylene), bonding agents, and resin composites on the gingival margin microleakage of class V composite restorations.

Materials and methods
Sixty premolars were sterilized and mounted in acrylic resin bases. Class V cavities were prepared buccally and lingually, 1 mm below the cementoenamel junction, comprising 12 groups (n = 10). In the experimental groups fiber inserts were cut and placed at the gingival seat, while the control groups had no inserts. Combinations of two composite materials, Filtek-Z250 and Filtek-LS (3M-ESPE), and four bonding agents, Clearfil SE bond (Kuraray) (C), Scotch Bond Multipurpose (3M-ESPE) (SB), Prime and Bond NT (Dentsply) (PB), and Filtek-LS (3M-ESPE) (LS) were used. Restorations were incrementally inserted and polymerized for 40s. Specimens were then stored in distilled water for 7 days and thermocycled for 500 cycles. Teeth surfaces were sealed with nail polish except for 1 mm around restoration margins and immersed in 2% red procion dye. Teeth were then sectioned buccolingually and dye penetration was assessed with five-point scale. Data were statistically-analyzed by Kruskal-Wallis, ANOVA and Tukey's tests (α = 5%).

Results
Mean microleakage scores varied from 0.40 (Groups C, C with polyethylene, LS, LS with polyethylene) to 1.50 (SB).

Conclusion
Different bonding agents led to differences in microleakage scores where C and LS showed significantly lower microleakage than PB. SB had highest mean microleakage score, however, incorporation of fibers resulted in significant reduction in microleakage.

Clinical significance
Class V resin composite restorations bonded with a total-etch adhesive had a significant reduction in mean microleakage scores when glass or polyethylene fibers were placed at the gingival cavo-surface margin. In contrast, for two self-etch adhesive systems, the incorporation of fibers had no significant effect on mean microleakage scores.

How to cite this article
Ahmed W, El-Badrawy W, Kulkarni G, Prakki A, El-Mowafy O. Gingival Microleakage of Class V Composite Restorations with Fiber Inserts. J Contemp Dent Pract 2013;14(4):622-628.
Cleaning Efficiency of Anatomic Endodontic Technology, ProFile System and Manual Instrumentation in Ovalshaped Root Canals: An in vitro Study
Authors: Reddy E Sujayeendranath , Sainath Dinapadu , Narenderreddy M , Pasari Srikanth , Vallikanthan Sangeetha , Sindhurareddy G 
Published: November 2013

ABSTRACT

Aim
This in vitro study is an attempt to compare the effectiveness in cleaning oval shaped root canals usingAnatomic Endodontic Technology (AET®), ProFile system® and Manual Instrumentation with K-files

Methodology
Sixty oval shaped single rooted maxillary and mandibular premolars with straight canals were divided in to three groups. The root canals were, confirmed as being oval shape by means of radiographs made in a buccolingual and mesiodistal direction. Automated canal preparation was performed using Anatomic Endodontic Technology (group 1) and the ProFile system® (group 2). Manual instrumentation (group 3) was performed with k-files. Irrigation was performed using alternatively 3% NaOCl and 17% EDTA, followed by rinsing with normal saline. The roots were split longitudinally into two halves and examined under a scanning electron microscope. The presence of debris and smear layer was recorded at distances 1, 5 and 10 mm from the working length using a three step scoring scale. Mean scores for debris and smear layer was calculated and statistically analyzed for between and within groups significance, using the Kruskal-Wallis nonparametric ANOVA test and Bonferroni's multiple comparison test.

Results
At 1, 5 and 10 mm levels the root canals prepared with AET had significantly less surface debris and smear layer on the canal walls as compared to canals prepared with ProFile system® or manual instrumentation. For all three groups significantly lower mean smear layer scores (p < 0.05) were recorded at 5 and 10 mm levels compared with the 1 mm level. Significantly lower mean debris scores (p < 0.05) were also recorded at 5 and 10 mm levels for the AET group whereas no significant differences were found between the three levels for the ProFile system® and manual instrumentation groups.

Conclusion
Although better instrumentation scores were obtained in canals prepared with AET, complete cleanliness was not achieved with any of the techniques and instruments investigated.

How to cite this article
Reddy ES, Sainath D, Narenderreddy M, Pasari S, Vallikanthan S, Sindhurareddy G. Cleaning Efficiency of Anatomic Endodontic Technology, ProFile System and Manual Instrumentation in Oval-shaped Root Canals: An in vitro Study. J Contemp Dent Pract 2013;14(4):629-634.
An in vivo Evaluation of Different Methods of Working Length Determination
Authors: Shah Nitin , Singh Sarita , Mandlik Jyoti , Pawar Kalpana , Gupta Paras , Shaik Shoaib Ali 
Published: November 2013

ABSTRACT

Objective
The purpose of this in vivo study was to compare the ability of digital tactile, digital radiographic and electronic methods to determine reliability in locating the apical constriction.

Materials and methods
Informed consent was obtained from patients scheduled for orthodontic extraction. The teeth were anesthetized, isolated and accessed. The canals were negotiated, pulp chamber and canals were irrigated and pulp was extirpated. The working length was then evaluated for each canal by digital tactile sensation, an electronic apex locator (The Root ZX) and digital radiography. The readings were then compared with post-extraction working length measurements.

Results
The percentage accuracy indicated that EAL method (Root ZX) shows maximum accuracy, i.e. 99.85% and digital tactile and digital radiographic method (DDR) showed 98.20 and 97.90% accuracy respectively.

Clinical significance
Hence, it can be concluded that the EAL method (Root ZX) produced most reliable results for determining the accurate working length.

How to cite this article
Mandlik J, Shah N, Pawar K, Gupta P, Singh S, Shaik SA. An in vivo Evaluation of Different Methods of Working Length Determination. J Contemp Dent Pract 2013;14(4):644-648.
A Scanning Electron Microscopic Study to evaluate the Efficacy of NaviTip FX in removing the Canal Debris during Root Canal Preparation: An in vitro Study
Authors: Nischith KG , Chandra Vinay , Gandi Padma , Shivanna Anil Kumar , Srinivas Siva , Himgiri S 
Published: November 2013

ABSTRACT

Objective
To evaluate the efficacy of NaviTip FX in removing the canal debris during root canal preparation using scanning electron microscopic study

Materials and methods
Thirty single rooted teeth with completely formed apices were used in this study. Standard endodontic access cavity preparations were performed. Then the teeth were randomly divided into two groups: groups 1 and 2 of 15 teeth each group. For group 1, NaviTip FX (brush covered needle) was used to irrigate the canal with 5.25% sodium hypochlorite after each instrument use. For group 2, NaviTip (brushless needle) was used for irrigation following each instrument use. ProTaper rotary files were used for the canal preparation. The teeth were then cleaned and dried before splitting them into two halves. The half with most visible part of the apex was used for scanning electron microscopic evaluation. The results were statistically analyzed using the Mann-Whitney U-test at significance level p < 0.005.

Results
The mean values for coronal and middle third of group 1 showed lower debris scores than group 2 and this difference was statistically significant at a p-value 0.01 and 0.05 respectively, but no significance difference between them at the apical third at a p-value of < 0.05.

Conclusion
The NaviTip FX (brush covered needle) showed effectively better canal wall debris removal than the NaviTip (brushless needle).

How to cite this article
Chandra V, Gandi P, Shivanna AK, Srinivas S, Himgiri S, Nischith KG. A Scanning Electron Microscopic Study to evaluate the Efficacy of NaviTip FX in removing the Canal Debris during Root Canal Preparation: An in vitro Study. J Contemp Dent Pract 2013;14(4):653-656.
Influence of Solvents and Composition of Etch-and-Rinse and Self-Etch Adhesive Systems on the Nanoleakage within the Hybrid Layer
Authors: Ferreira Joao Cardoso , Pires Patricia Teixeira , Azevedo Alvaro Ferreira , Oliveira Sofia Arantes , Melo Paulo Ribeiro , Silva Mario Jorge 
Published: November 2013

ABSTRACT

Aim
The goal of this study was to evaluate nanoleakage within the hybrid layer yielded by etch-and-rinse and self-etch adhesive systems, with different solvents and compositions.

Materials and methods
Four adhesives were applied onto 20 human dentin disks: group A: Adper Scotchbond 1XT™ (3M ESPE), group B: One Coat Bond® (Coltène Whaledent), group C: AdheSE® (Ivoclar Vivadent) and group D: Xeno-V® (Dentsply). The samples were immersed in aqueous ammoniacal silver nitrate for 24 hour, prepared and observed under field-emission scanning electron microscopy with backscattered electrons. Microphotographs were scanned and data were processed. The mean value and standard deviation were calculated. KruskalWallis and Mann-Whitney tests were used (p < 0.05).

Results
All the adhesives showed nanoleakage within the hybrid layer: Adper Scotchbond 1XT™ (218.5 μm ± 52.6 μm), One Coat Bond® (139.6 μm ± 79.0 μm), AdheSE® (92.7 μm ± 64.8 μm) and Xeno-V® (251.0 μm ± 85.2 μm). AdheSE® yielded less nanoleakage than Adper Scotchbond-1XT™ (p = 0.003) and than Xeno-V® (p = 0.007). No other statistically significant differences were detected.

Conclusion
Two-step self-etch adhesive system (AdheSE®) might contribute for lower nanoleakage deposition and thus better performance in dentin adhesion.

Clinical significance
The two-step self-etch adhesive system showed the lowest nanoleakage deposition compared with the other adhesive systems evaluated, which seems to indicate a better behavior when a restoration is performed in dentin and possibly can lead to a durable adhesion along time.

How to cite this article
Ferreira JC, Pires PT, Azevedo AF, Oliveira SA, Melo PR, Silva MJ. Influence of Solvents and Composition of Etch-and-Rinse and Self-Etch Adhesive Systems on the Nanoleakage within the Hybrid Layer. J Contemp Dent Pract 2013;14(4):691-699.
Shear Bond Strength of Two Adhesive Materials to Eroded Enamel
Authors: Hesse Daniela , Lenzi Tathiane , Guglielmi Camila , Anacleto Ketlin , Raggio Daniela Prócida 
Published: November 2013

ABSTRACT

Aim
To evaluate the bond strength of one etch-and-rinse adhesive system and one resin-modified glass ionomer cement to sound and eroded enamel.

Materials and methods
Forty-eight bovine incisors were embedded in acrylic resin and ground to obtain flat buccal enamel surfaces. Half of the specimens were submitted to erosion challenge with pH-cycling model (3x/cola drink for 7 days) to induce eroded enamel. After that, all specimens were randomly assigned according to adhesive material: etch-andrinse adhesive system (Adper Single Bond 2 – 3M ESPE, USA) or resin-modified glass ionomer cement (Vitro Fil LC – DFL, Brazil). The shear bond testing was performed after 24 hours water storage (0.5 mm/min). Shear bond strength means were analyzed by two-way ANOVA and Tukey post hoc tests (p < 0.05).

Results
Adper Single Bond 2 showed the highest bond strength value to eroded enamel (p < 0.05), whereas no difference was observed in sound enamel compared with Vitro Fil LC (p > 0.05).

Conclusion
Bond strength of etch-and-rinse adhesive system increases in eroded enamel, while no difference is verified to resin-modified glass ionomer cement.

Clinical significance
Adhesive materials may be used in eroded enamel without jeopardizing the bonding quality; however it is preferable to use etch-and-rinse adhesive system.

How to cite this article
Lenzi T, Hesse D, Guglielmi C, Anacleto K, Raggio DP. Shear Bond Strength of Two Adhesive Materials to Eroded Enamel. J Contemp Dent Pract 2013;14(4):700-703.
Comparison of the Remaining Dentin Thickness in the Root after Hand and Four Rotary Instrumentation Techniques: An in vitro Study
Authors: Issac Johnson K , Rao MS Rama , Shameem Abdul , Nair Rashmi , Ghanta Sureshbabu , Thankachan Rekha P 
Published: November 2013

ABSTRACTAimThe aim of the present study was to compare the remaining dental thickness (RDT) in the mesiobuccal root of mandibular first molars at 3 and 7 mm from the anatomic apex after instrumentation with ProTaper, light speed LSX, K3 and M2 and to compare with that of K-files.Materials and methodsIn this study, 60 extracted, untreated human mandibular first molars with fully formed apices, with curvature less than 35° and no root resorption were used. Prepared specimens were cut horizontally at 3 and 7 mm short of anatomic apex. The least dentin thickness from canal to external root surface was observed under 3× magnification and recorded using Clemax measuring tool and the sections were reassembled. Group I—instrumentation with ProTaper, group II—instrumentation with K3, group III—instrumentation with Light Speed LSX, group IV—instrumentation with M2 and group V— instrumentation with K-files and RDT was measured.ResultsResults showed that group V removed lesser amount of dentin compared to all other groups while all the three instrumentation techniques removed almost equal amount of dentin apically.Clinical significanceCleaning and shaping of the root canal space involves the elimination of pathogenic contents as well as attaining a uniform specific shape. However, the RDT following the use of various intraradicular procedures is an important factor to be considered as an iatrogenic cause that may result in root fracture. To avoid this, newer rotary instruments are being introduced.How to cite this articleRao MSR, Shameem A, Nair R, Ghanta S, Thankachan RP, Issac JK. Comparison of the Remaining Dentin Thickness in the Root after Hand and Four Rotary Instrumentation Techniques: An in vitro Study. J Contemp Dent Pract 2013;14(4):712-717.
Prevalence of Dental Caries and Oral Hygiene Status Among School Going Children: An Epidemiological Study
Authors: Jayapalan CS , Gondhalekar Rajesh V , Krishna B Jaya , Shaloob KM Muhamed , Ummer P Fajar 
Published: November 2013

ABSTRACT
Oral health is an important part of general health of body. Oral hygiene determines oral health status. Thus, oral hygiene is most important for good health in general. Poor oral hygiene can be source of many diseases. By maintaining the good oral hygiene, we can prevent occurrence of many disease. A survey was carried out to assess oral hygiene status and to find out caries prevalence rate among school going children of age 6 to 12 years. 957 healthy subjects including 567 boys and 390 girls from four different schools were examined in broad day light with the help of mouth mirror and explorer.

How to cite this article
Ravishankar PL, Jayapalan CS, Gondhalekar RV, Krishna BJ, Shaloob KMM, Ummer PF. Prevalence of Dental Caries and Oral Hygiene Status Among School Going Children: An Epidemiological Study. J Contemp Dent Pract 2013;14(4):743-746.
Dental Health and Treatment Needs Among Children in a Tribal Community
Authors: Kathariya Mitesh D , Viragi Prashant S , Dwijendra KS , Chopra Kirti , Dadpe Mahesh V , Madhukar HS 
Published: November 2013

ABSTRACT

Objective
To assess the dental health status and treatment needs among children of ‘Pardhi’ tribal community.

Methods
A total of 185 children were examined over a period of 2 months using WHO proforma. The statistical software namely SPSS version 15.0 and data was analyzed using Student's t-test and ANOVA test at p < 0.05.

Results
The mean score for dft and DMFT was 1.87 ± 1.073 and 2.04 ± 1.564 respectively with males subjects were having comparatively more scores. It was also found significant differences between age groups. Most of the children needed one surface filling, i.e. 29.40%, followed by pulp care and restoration (19.30%), two or more surface fillings (15.60%) and extraction (11.70%).

Clinical significance
The study subjects were characterized by a lack of dental care services, high prevalence of dental caries and treatment needs. Therefore, implementation of a basic oral health care program for this tribal population is a high priority

How to cite this article
Viragi PS, Dwijendra KS, Kathariya MD, Chopra K, Dadpe MV, Madhukar HS. Dental Health and Treatment Needs Among Children in a Tribal Community. J Contemp Dent Pract 2013;14(4):747-750.
Secondary Closure of Alveolar Clefts with Mandibular Symphyseal Bone Grafts and with Platelet-Rich Fibrin Under Local Anesthesia: Three Case Reports
Authors: Findik Yavuz , Baykul Timuçin 
Published: November 2013

ABSTRACT
Secondary alveolar bone grafting is a method that enables an excellent oral rehabilitation of the patients having cleft palate. Many types of bone grafts have been used for reconstruction of the cleft, including autogenous bone and bone substitutes. Platelet-rich fibrin (PRF) has been shown to be effective in grafting the defect. The aim of this presentation is to report the closure of an alveolar cleft with the use of symphyseal bone grafts harvested with platelet rich fibrin under local anesthesia. PRF may be a good treatment choice depending on the early radiographical view of the defect and uneventfull healing.

How to cite this article
Flndlk Y, Baykul T. Secondary Closure of Alveolar Clefts with Mandibular Symphyseal Bone Grafts and with Platelet-Rich Fibrin Under Local Anesthesia: Three Case Reports. J Contemp Dent Pract 2013;14(4):751-753.