bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does a coexisting congener of a mixed mating species affect the genetic structure and selfing rate via reproductive interference?
Authors: Katsuhara Koki R. , Ushimaru Atushi , Miyazaki Yuko 
Published: August 2024

AbstractReproductive interference is defined as an interspecific interaction that reduces fitness via mating processes. Although its ecological and evolutionary consequences have attracted much attention, how reproductive interference affects the population genetic structures of interacting species is still unclear. In flowering plants, recent studies found that self-pollination can mitigate the negative effects of reproductive interference. Selfing-biased seed production is expected to increase population-level inbreeding and the selfing rate, and limits gene flow via pollinator outcrossing among populations. We examined the population genetics of the mixed-mating annual herb Commelina communis f. ciliata, focusing on reproductive interference by the sympatric competing congener C. communis using microsatellite markers. First, we found that almost all C. c. f. ciliata populations had relatively high inbreeding coefficients. Then, comparing sympatric and allopatric populations, we found evidence that reproductive interference from a competing congener increased the inbreeding coefficient and selfing rate. Allopatric populations exhibit varied selfing rates while almost all sympatric populations exhibit extremely high selfing rates, suggesting that population selfing rates were also influenced by unexamined factors, such as pollinator limitation. Besides, our findings revealed that reproductive interference from a competing congener did not limit gene flow among populations. We present the first report on how reproductive interference affects the genetic aspects of populations. Our results suggested that the high selfing rate of C. c. f. ciliata promotes its sympatric distribution with C. communis, even in the presence of reproductive interference, although it is not clear whether reproductive interference directly causes the high selfing rate.
Robotic bariatric surgery reduces morbidity for revisional gastric bypass when compared to laparoscopic: outcome of 8-year MBSAQIP analysis of over 40,000 cases
Authors: Spurzem Graham J. , Broderick Ryan C. , Kunkel Emily K. , Hollandsworth Hannah M. , Sandler Bryan J. , Jacobsen Garth R. , Horgan Santiago 
Published: August 2024

Abstract
Introduction
Robotic-assisted metabolic and bariatric surgery (MBS) is gaining popularity. Revisional MBS is associated with higher perioperative morbidity compared to primary MBS. The optimal surgical approach to minimize complications in these complex cases is unclear. The goal of this study was to assess robot utilization in revisional MBS and compare laparoscopic and robotic revisional MBS outcomes in the Metabolic and Bariatric Surgery Accreditation and Quality Improvement Program (MBSAQIP) database.

Methods
A retrospective review of the MBSAQIP database was performed identifying revisional sleeve gastrectomy (SG) and Roux-en-Y gastric bypass (RYGB) cases from 2015 to 2022. Primary MBS, open/emergent cases, cases converted to another approach, and combined cases other than esophagogastroduodenoscopy were excluded. 30-Day outcomes for laparoscopic and robotic cases were compared using multivariate logistic regression adjusting for patient demographics, comorbidities, and operative variables.

Results
41,404 Cases (14,474 SG; 26,930 RYGB) were identified. From 2015 to 2022, the percentage of revisional SG and RYGB cases performed robotically increased from 6.1% and 7.3% to 24.2% and 32.0% respectively. Laparoscopic SG had similar rates of overall morbidity, leak, bleeding, readmission, reoperation, and length of stay compared to robotic. Laparoscopic RYGB had significantly higher rates of overall morbidity (6.2% vs. 4.8%, p < 0.001, AOR 0.80 [0.70–0.93]), blood transfusion (1.5% vs. 1.0%, p < 0.05, AOR 0.74 [0.55–0.99]), superficial incisional SSI (1.2% vs. 0.4%, p < 0.001, AOR 0.30 [0.19–0.47]), and longer length of stay (1.87 vs. 1.76 days, p < 0.001) compared to robotic. Laparoscopic operative times were significantly shorter than robotic (SG: 86.4 ± 45.8 vs. 113.5 ± 51.7 min; RYGB: 130.7 ± 64.7 vs. 165.5 ± 66.8 min, p < 0.001).

Conclusion
Robot utilization in revisional bariatric surgery is increasing. Robotic surgery has lower postoperative morbidity and shorter length of stay in revisional RYGB when compared to laparoscopic. Robotic platforms may have the capacity to improve the delivery of care for patients undergoing revisional bariatric surgery.
Association of ambient temperature with intentional self-harm and suicide death in Seoul: a case-crossover design with a distributed lag nonlinear model
Authors: Kim Seunghyeon , Kim Yoonhee , Park Eunsik 
Published: August 2024

AbstractPrevious epidemiological studies have reported a short-term association between ambient temperature and suicide risk. To gain a clearer understanding of this association, it is essential to differentiate the risk factors for intentional self-harm (ISH) from those specifically associated with suicide deaths. Therefore, this study aims to examine whether the association between daily temperature and ISH or suicide deaths differs by age and sex. Between 2014 and 2019, cases of emergency room visits related to ISH and suicide deaths in Seoul were identified. A time-stratified case-crossover design was used to adjust for temporal trends and seasonal variation. A distributed lag nonlinear model was used to analyze the nonlinear and time-delayed effect of ambient temperature on ISH and suicide deaths. Positive associations were observed between temperature and both ISH and suicide deaths. For ISH, the relative risk (RR) was high at 1.17 (95% confidence interval (CI): 1.03, 1.34) for a temperature of 25.7 °C compared with 14.8 °C. The RR for suicide death was higher than those for ISH, at 1.43 (95% CI: 1.03, 2.00) for a temperature of 33.7 °C. These associations varied by age and sex, with males and females aged 35–64 years showing increased susceptibility to suicide deaths. This study provides detailed evidence that unusually high temperatures, both anomalous and out of season, may trigger suicidal behaviors, including both ISH and suicide deaths.
Patterns and Predictors of Recurrence After Curative Resection of Colorectal Liver Metastasis (CRLM)
Authors: Vadisetti Satya Niharika , Kazi Mufaddal , Patkar Shraddha , Mundhada Rohit , Desouza Ashwin , Saklani Avanish , Goel Mahesh 
Published: August 2024

Abstract
Background
Our study aims to determine the predictors and patterns of relapses after curative colorectal liver metastasis (CRLM) resection.

Methods
A single-centre, retrospective study of CRLM patients operated between 2010 and 2022 was performed. The site of first recurrence was either hepatic (marginal (≤ 1 cm) or extramarginal), extrahepatic, or both. Factors that predicted relapse patterns and overall survival were determined by multivariable Cox regression analysis with backward elimination of variables.

Results
The study consisted of 258 patients, with a similar proportion of synchronous (144; 56%) and metachronous(114; 43%) metastasis. At a 43-month median follow-up, 156 patients (60.4%) developed recurrences with 33 (21.1%) in the liver, 62(24.03%) extra-hepatic recurrences, and 58 (22.48%) having both. Isolated marginal liver relapses were seen in seven (9.89%) liver recurrence patients. The median overall and relapse-free survivals were 38 months (30–54) and 13 months (11–16), respectively. The 3-year liver-relapse-free survival was 54.4% (44.9–60.6). Size of liver metastases > 5 cm (HR 2.06 (1.34–3.17), involved surgical margins (HR 2.16 (1.27–3.68)), and adjuvant chemotherapy (HR 1.89 (1.07–3.35)) were predictors of hepatic recurrences. Node positivity of primary (HR 1.61 (1.02–2.56)), presence of baseline extra-hepatic metastases (HR 0.30 (0.18–0.51)), size of liver metastases > 5 cm (HR 2.02 (1.37–2.99)), poorly differentiated histology (HR 2.25 (1.28–3.49)), presence of LVI (HR 2.25 (1.28–3.94)), and adjuvant chemotherapy (HR 2.15 (1.28–3.61)) were predictors of extra-hepatic recurrences.

Conclusion
The study found majority relapses occurred at extrahepatic sites whilst isolated marginal recurrences were few. The consistent predictors of recurrence were size and inability to deliver adjuvant therapy. A tailored adjuvant therapy might improve outcomes after liver metastasectomy in colorectal cancers.
Design and Assessment of Fighter Pilot Assistance Systems for Air-to-Air Refuelling with Probe-and-Drogue-Equipment
Authors: Ament J. , Lachmann J. , Schmelz J. , Wandrey L. 
Published: August 2024

AbstractAir-to-air refuelling (AAR) with a probe-and-drogue system is a highly demanding process for fighter pilots. In the frame of the project “Future Air-to-Air Refuelling” (F(AI)2R) the German Aerospace Center (DLR) is designing concepts for assistance systems to support fighter pilots during this manoeuvre. The AAR process was examined using a hierarchical task analysis (HTA) based on a literature review and semi-structured interviews with Eurofighter-, Tornado- and test pilots. “Situation Awareness Requirements” (SAR), which represent relevant parameters for the situation awareness of the fighter pilot were derived from the HTA and rated by pilots according to their relevance. The collected data was then used to design different concepts of pilot assistance systems using a morphological box and a decision matrix. The concepts were visualised as storyboards, assessed by test pilots of the “Wehrtechnische Dienststelle 61” (WTD 61) and optimized based on their feedback. In this paper, the used methodology as well as the results of the HTA and SAR analysis are presented. Besides that, storyboards for pilot assistance systems displaying the overtake speed “German Patent No. 10.2022.110.505” and the “Contact Assistance, Status and Warning System” (CASWS) “German Patent No. 10.2022.123.653” are described. Additionally, the planned implementation using a HoloLens 2 is explained and the strategy for the subsequent simulator study on the DLR fighter aircraft simulator “Military AiR vehicle Simulator – Fast Integration Testbed” (MARS-FIT) is described.
Integration of microfluidic chips with biosensors
Authors: Sekhwama Masindi , Mpofu Kelvin , Sudesh Sivarasu , Mthunzi-Kufa Patience 
Published: August 2024

AbstractThe potential threat posed by disease outbreaks to diagnostic instruments demands the development of more effective biosensor technologies to counteract the risks. Diseases like SARS-CoV-2, Ebola, malaria, cholera, and many more have demonstrated beyond the limits of health care that new advancements are required for early detection and diagnosis. The rising number of diseases outbreaks has led to an increasing demand for biosensors that are more effective and quicker to utilize in healthcare settings. A biosensor incorporated with microfluidic chips offers an improved detection compared to traditional or classical biosensors. Microfluidic chips improve the performance of the biosensors by allowing automation, mixing, separation, throughput processing, and transport of the analytes to desired reactors. A biosensor incorporated with a microfluidic chip has improved sensitivity, easy operation and can use small volumes of samples to process the results. The effectiveness of biosensors depends also on the materials used in its fabrication and there are many materials used for fabrication which are reviewed in this work. This paper reviews the potential advantages of the use of microfluidic chips to enhance the performance of biosensors, materials used to fabricate the chips, and potential electrodes incorporated into microfluidic chips which improve the detection time by shortening the processing time for biosensors at the point of care service. This work also reviews new technologies which are not previously addressed other reviews including, integration of cell-imprinted polymers with microfluidic sensors and delved into future technologies outlook.
A narrative review: integrating leisure time programs for children with hearing impairment to mitigate behavioral problems
Authors: Fayed Gamal 
Published: August 2024

AbstractThis paper focused on students' leisure time with an exclusive focus on students with hearing disabilities leading to behavioural issues. This sensitive issue needs time to be highlighted as students with such disabilities are naturally restrained from utilizing their leisure time. This research aims to investigate numerous courses of action in the light of a detailed literature review and explore possible ways to reduce their severity and mitigate their effects. This qualitative conceptual review encompasses on how leisure time programs could be integrated and inclusive into the education, workplace, and daily life of children with hearing disabilities. This research effectively offers suggestions and recommendations that could help teachers and parents while dealing with leisure time as an approach to reducing behavioural problems such as practical solutions on various tools and procedures that can be manipulated and used in various workplaces. This review has numerous implications, such as this study could serve as a premier in setting guidelines for applying a leisure time approach to the non-clinical work environment.
Does poor oral health impact on young children's development? A rapid review
Authors: Watt Samantha , Dyer Tom A. , Marshman Zoe , Jones Kate 
Published: August 2024

AbstractBackground The extent to which poor oral health in young children impacts developmental milestones is unclear.Aim To review the evidence of association between poor oral health and development of young children (0-5 years).Method A rapid review was conducted. Medline was searched for articles in English published from 2000 onwards. The outcomes considered were the impact of poor oral health on speech and language development, oral health-related quality of life (OHRQoL), school performance and attendance.Results In total, 15 studies were included: four systematic reviews, a critical review and 10 primary studies. All data were from observational or cross-sectional studies. Premature extraction of primary maxillary incisors was associated with change in speech sounds and articulation. Two primary studies found associations between early childhood caries and physical and psychomotor deficiencies, while acknowledging a web of causation with other factors, including socioeconomic status. Children with dental caries reported lower OHRQoL. Studies found caries experience was associated with preschool absenteeism.Conclusion Poor oral health in young children appears to be associated with speech and language development, OHRQoL, and school attendance and performance. However, there are significant limitations in the existing evidence, with further high-quality, observational, longitudinal research required to fully understand the impact of poor oral health on the development of young children. Strategies to improve oral health by health and education professionals are needed to ensure young children reach their potential.
EZH2 represses mesenchymal genes and upholds the epithelial state of breast carcinoma cells
Authors: Gallardo Amador , López-Onieva Lourdes , Belmonte-Reche Efres , Fernández-Rengel Iván , Serrano-Prados Andrea , Molina Aldara , Sánchez-Pozo Antonio , Landeira David 
Published: August 2024

AbstractEmerging studies support that the polycomb repressive complex 2 (PRC2) regulates phenotypic changes of carcinoma cells by modulating their shifts among metastable states within the epithelial and mesenchymal spectrum. This new role of PRC2 in cancer has been recently proposed to stem from the ability of its catalytic subunit EZH2 to bind and modulate the transcription of mesenchymal genes during epithelial-mesenchymal transition (EMT) in lung cancer cells. Here, we asked whether this mechanism is conserved in other types of carcinomas. By combining TGF-β-mediated reversible induction of epithelial to mesenchymal transition and inhibition of EZH2 methyltransferase activity, we demonstrate that EZH2 represses a large set of mesenchymal genes and favours the residence of breast cancer cells towards the more epithelial spectrum during EMT. In agreement, analysis of human patient samples supports that EZH2 is required to efficiently repress mesenchymal genes in breast cancer tumours. Our results indicate that PRC2 operates through similar mechanisms in breast and lung cancer cells. We propose that PRC2-mediated direct transcriptional modulation of the mesenchymal gene expression programme is a conserved molecular mechanism underlying cell dissemination across human carcinomas.
Characterizing barren plateaus in quantum ansätze with the adjoint representation
Authors: Fontana Enrico , Herman Dylan , Chakrabarti Shouvanik , Kumar Niraj , Yalovetzky Romina , Heredge Jamie , Sureshbabu Shree Hari , Pistoia Marco 
Published: August 2024

AbstractVariational quantum algorithms, a popular heuristic for near-term quantum computers, utilize parameterized quantum circuits which naturally express Lie groups. It has been postulated that many properties of variational quantum algorithms can be understood by studying their corresponding groups, chief among them the presence of vanishing gradients or barren plateaus, but a theoretical derivation has been lacking. Using tools from the representation theory of compact Lie groups, we formulate a theory of barren plateaus for parameterized quantum circuits whose observables lie in their dynamical Lie algebra, covering a large variety of commonly used ansätze such as the Hamiltonian Variational Ansatz, Quantum Alternating Operator Ansatz, and many equivariant quantum neural networks. Our theory provides, for the first time, the ability to compute the exact variance of the gradient of the cost function of the quantum compound ansatz, under mixing conditions that we prove are commonplace.
Structure of anellovirus-like particles reveal a mechanism for immune evasion
Authors: Liou Shu-hao , Boggavarapu Rajendra , Cohen Noah R. , Zhang Yue , Sharma Ishwari , Zeheb Lynn , Mukund Acharekar Nidhi , Rodgers Hillary D. , Islam Saadman , Pitts Jared , Arze Cesar , Swaminathan Harish , Yozwiak Nathan , Ong Tuyen , Hajjar Roger J. , Chang Yong , Swanson Kurt A. , Delagrave Simon 
Published: August 2024

AbstractAnelloviruses are nonpathogenic viruses that comprise a major portion of the human virome. Despite being ubiquitous in the human population, anelloviruses (ANVs) remain poorly understood. Basic features of the virus, such as the identity of its capsid protein and the structure of the viral particle, have been unclear until now. Here, we use cryogenic electron microscopy to describe the first structure of an ANV-like particle. The particle, formed by 60 jelly roll domain-containing ANV capsid proteins, forms an icosahedral particle core from which spike domains extend to form a salient part of the particle surface. The spike domains come together around the 5-fold symmetry axis to form crown-like features. The base of the spike domain, the P1 subdomain, shares some sequence conservation between ANV strains while a hypervariable region, forming the P2 subdomain, is at the spike domain apex. We propose that this structure renders the particle less susceptible to antibody neutralization by hiding vulnerable conserved domains while exposing highly diverse epitopes as immunological decoys, thereby contributing to the immune evasion properties of anelloviruses. These results shed light on the structure of anelloviruses and provide a framework to understand their interactions with the immune system.
Unique genetic and risk-factor profiles in clusters of major depressive disorder-related multimorbidity trajectories
Authors: Gezsi Andras , Van der Auwera Sandra , Mäkinen Hannu , Eszlari Nora , Hullam Gabor , Nagy Tamas , Bonk Sarah , González-Colom Rubèn , Gonda Xenia , Garvert Linda , Paajanen Teemu , Gal Zsofia , Kirchner Kevin , Millinghoffer Andras , Schmidt Carsten O. , Bolgar Bence , Roca Josep , Cano Isaac , Kuokkanen Mikko , Antal Peter , Juhasz Gabriella 
Published: August 2024

AbstractThe heterogeneity and complexity of symptom presentation, comorbidities and genetic factors pose challenges to the identification of biological mechanisms underlying complex diseases. Current approaches used to identify biological subtypes of major depressive disorder (MDD) mainly focus on clinical characteristics that cannot be linked to specific biological models. Here, we examined multimorbidities to identify MDD subtypes with distinct genetic and non-genetic factors. We leveraged dynamic Bayesian network approaches to determine a minimal set of multimorbidities relevant to MDD and identified seven clusters of disease-burden trajectories throughout the lifespan among 1.2 million participants from cohorts in the UK, Finland, and Spain. The clusters had clear protective- and risk-factor profiles as well as age-specific clinical courses mainly driven by inflammatory processes, and a comprehensive map of heritability and genetic correlations among these clusters was revealed. Our results can guide the development of personalized treatments for MDD based on the unique genetic, clinical and non-genetic risk-factor profiles of patients.
Femtosecond laser writing of ant-inspired reconfigurable microbot collectives
Authors: Ren Zhongguo , Xin Chen , Liang Kaiwen , Wang Heming , Wang Dawei , Xu Liqun , Hu Yanlei , Li Jiawen , Chu Jiaru , Wu Dong 
Published: August 2024

AbstractMicrobot collectives can cooperate to accomplish complex tasks that are difficult for a single individual. However, various force-induced microbot collectives maintained by weak magnetic, light, and electric fields still face challenges such as unstable connections, the need for a continuous external stimuli source, and imprecise individual control. Here, we construct magnetic and light-driven ant microbot collectives capable of reconfiguring multiple assembled architectures with robustness. This methodology utilizes a flexible two-photon polymerization strategy to fabricate microbots consisting of magnetic photoresist, hydrogel, and metal nanoparticles. Under the cooperation of magnetic and light fields, the microbots can reversibly and selectively assemble (e.g., 90° assembly and 180° assembly) into various morphologies. Moreover, we demonstrate the ability of assembled microbots to cross a one-body-length gap and their adaptive capability to move through a constriction and transport microcargo. Our strategy will broaden the abilities of clustered microbots, including gap traversal, micro-object manipulation, and drug delivery.
Iron-catalyzed radical Markovnikov hydrohalogenation and hydroazidation of alkenes
Authors: Elfert Jonas , Frye Nils Lennart , Rempel Isabel , Daniliuc Constantin Gabriel , Studer Armido 
Published: August 2024

AbstractWe herein report radical hydroazidation and hydrohalogenation of mono-, di- and trisubstituted alkenes through iron catalysis. The alkene moiety that often occurs as a functionality in natural products is readily transformed into useful building blocks through this approach. Commercially available tosylates and α-halogenated esters are used as radical trapping reagents in combination with silanes as reductants. The reported radical Markovnikov hydroazidation, hydrobromination, hydrochlorination, and hydroiodination occur under mild conditions. These hydrofunctionalizations are valuable and practical alternatives to ionic hydrohalogenations with the corresponding mineral acids that have to be run under harsher acidic conditions, which diminishes the functional group tolerance. Good to excellent diastereoselectivities can be obtained for the hydrofunctionalization of cyclic alkenes.