bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal serum protein profile and immune response protein subunits as markers for non‐invasive prenatal diagnosis of trisomy 21, 18, and 13
Authors: Narasimhan Kothandaraman , Lin Su Lin , Tong Terry , Baig Sonia , Ho Sherry , Sukumar Ponnusamy , Biswas Arijit , Hahn Sinuhe , Bajic Vladimir B. , Choolani Mahesh 
Published: February 2013

ABSTRACTObjectivesTo use proteomics to identify and characterize proteins in maternal serum from patients at high‐risk for fetal trisomy 21, trisomy 18, and trisomy 13 on the basis of ultrasound and maternal serum triple tests.MethodsWe performed a comprehensive proteomic analysis on 23 trisomy cases and 85 normal cases during the early second trimester of pregnancy. Protein profiling along with conventional sodium dodecyl sulfate polyacrylamide gel electrophoresis/Tandem mass spectrometry analysis was carried out to characterize proteins associated with each trisomy condition and later validated using Western blot.ResultsProtein profiling approach using surface enhanced laser desorption/ionization time‐of‐flight mass (SELDI‐TOF/MS) spectrometry resulted in the identification of 37 unique hydrophobic proteomic features for three trisomy conditions. Using sodium dodecyl sulfate polyacrylamide gel electrophoresis followed by Matrix Assisted Laser Desorption Ionization – Time of Flight/Time of Flight (MALDI‐TOF/TOF) and western blot, glyco proteins such as alpha‐1‐antitrypsin, apolipoprotein E, apolipoprotein H, and serum carrier protein transthyretin were identified as potential maternal serum markers for fetal trisomy condition. The identified proteins showed differential expression at the subunit level. .ConclusionsMaternal serum protein profiling using proteomics may allow non‐invasive diagnostic testing for the most common trisomies and may complement ultrasound‐based methods to more accurately determine pregnancies with fetal aneuploidies. © 2013 John Wiley & Sons, Ltd.
Dielectric and electromechanical studies of plasticized poly(vinyl chloride) fabricated from plastisol
Authors: Ali Mohammad , Ueki Takamitsu , Hirai Toshihiro , Sato Takaaki , Sato Tamotsu 
Published: February 2013

AbstractPoly(vinyl chloride) (PVC) plastisol is used in many industrial applications and is considered an electrically inactive material. To explore the potential of plastisol as an electroactive material, the dielectric properties, space charge distribution, mechanical properties, internal structure and electromechanical behavior of plasticized PVC (PVC gel) prepared from plastisol by heating were investigated. The gel exhibited a large dielectric constant at low frequencies (1–1000 Hz), an asymmetric charge distribution and excellent mechanical properties. Various DC electric fields were applied to the gel placed parallel between two electrodes and the electrostatic adhesive force to the anode was measured. The results of small‐ and wide‐angle X‐ray scattering suggested that the electromechanical properties of the gel originated from the characteristics of the physical crosslinking distance (ca 20 nm) of PVC in the gel structure. Considering the dielectric properties, space charge density and adhesion force to the anode, PVC gels prepared from plastisol using the heating method have potential for use as electroactive materials. © 2013 Society of Chemical Industry
Continuous Coaxial Electrohydrodynamic Atomization System for Water‐Stable Wrapping of Magnetic Nanoparticles
Authors: Kim Sang‐Yoon , Yang Jaemoon , Kim Bongjune , Park Jungmin , Suh Jin‐Suck , Huh Yong‐Min , Haam Seungjoo , Hwang Jungho 
Published: February 2013

AbstractAn electrohydrodynamic atomization (EHDA) system that generates an electrospray can achieve particle formation and encapsulation by accumulating an electric charge on liquid flowing out from the nozzle. A novel coaxial EHDA system for continuous fabrication of water‐stable magnetic nanoparticles (MNPs) is established, based on a cone‐jet mode of electrospraying. Systemic variables, such as flow rates from dual nozzles and inducing voltages, are controlled to enable the preparation of water‐soluble MNPs coated by polysorbate 80. The PEGylated MNPs exhibit water stability. The magnetic resonance imaging potential of these MNPs is confirmed by in vivo imaging using a gastric cancer xenograft mouse model. Thus, this advanced coaxial EHDA system demonstrates remarkable capabilities for the continuous encapsulation of MNPs to render them water‐stable while preserving their properties as imaging agents.
Neue Eisenbahnbrücke am Bahnhof Hamburg‐Dammtor
Authors: Starke Peter 
Published: February 2013

AbstractDer Bericht befasst sich mit der Erneuerung einer vierteiligen Eisenbahnbrücke “unter dem rollenden Rad“, die neben dem Durchgangsbahnhof “Dammtor“ an einem belebten Straßenknotenpunkt gelegen ist (Bilder 1 und 2). Zur Erhaltung der vorhandenen Ost‐West‐Verbindung innerhalb der Stadt sind diese Arbeiten im Jahr 2010 ausgeschrieben worden und wurden im November 2012 abgeschlossen. Beim Altbau handelt es sich um eine Stahlkonstruktion auf Stützen von 1902, die als Vollwandträger mit Nietverbindungen ausgebildet ist und nach jetzt 110 Jahren noch den vollen Bahnbetrieb einwandfrei ertragen hat. Im neuen Entwurf sollen die Stützen entfallen, dafür aber die Wände erneuert werden. Die Brücke ist schiefwinklig 30 bis 40° und im Grundriss zu den Bahnsteigen hin leicht fächerförmig ausgebildet.Der unmittelbar angrenzende Bahnhof hat zwei Bahnsteige innerhalb einer geschlossenen Halle mit stählernem Gewölbedach. Die Bahnsteige dienen dem Fernverkehr Hamburg–Berlin und dem Stadtbahnverkehr Hauptbahnhof–Altona.Es werden unter anderem die Bauhilfsmaßnahmen beschrieben, die zur Erstellung der neuen Widerlagerwände erforderlich waren. Den Abschluss bilden einige Bilder vom Transport der trogförmigen Überbauten zur Einbaustelle.New railway bridge at station Hamburg‐Dammtor. The following report concernes the renewing of a four part railway bridge “beyond the rolling wheels“, which is situated besides the through‐traffic station “Dammtor“ near a vivid street knot (Figs. 1 and 2). To beware east‐west connection within the town, this project was advertised in 2010 and will be finished at the end of 2012. The old bridge has been a steel‐construction on columns from 1902. It was formed as a sheet metal girder with riveted connections. The bridge has carried the full railwayload after now 110 years. In future the 16 columns were to be released, but the walls were to be renewed.The bridge is oblique‐angled 30 to 40 degrees and in the ground plan lightly fan shaped leading to the platforms. The directly bordering station has two platforms. They are serving for the long distance traffic Hamburg–Berlin and for the town traffic Mainstation–Altona, both within a closed hall with a steel‐vault roof. Described are among others the devices, which were needed for rising the new walls under the bridge. Finally there are pictures about the transport oft he prefabricated trough shaped superstructures.
Ersatzneubau für die Schnettkerbrücke in Dortmund: Bauwerksentwurf (Teil 1)
Authors: Hamme Markus , Prenting Adele , Neumann Winfried 
Published: February 2013

AbstractIm Zuge des Ausbaus der Autobahn A 40 wurde ein Ersatzneubau für die Schnettkerbrücke in Dortmund erforderlich. Neben den technischen Schwierigkeiten, die sich aus dem Bauen unter Aufrechthaltung des Verkehrs ergeben, war die Bauaufgabe auch durch besondere Anforderungen an die Gestaltung der neuen Brücke geprägt. Die außergewöhnliche Lösung ist eine technisch und optisch anspruchsvolle Brücke, bei der verschiedene Tragwirkungen miteinander kombiniert werden. Die Ausführung erfolgt als robuste Stahlverbundbrücke mit einer maximalen Stützweite von 132 m.Replacement of the Schnettker Bridge in Dortmund: Design of the bridge (part 1). In course of the expansion of the autobahn A 40 the bridge Schnettkerbrücke had to be replaced. Beside of technical difficulties, resulting from the request to build the new bridge while maintaining the traffic, there have been a lot of architectural requirements to the new bridge. The extraordinary solution is a technical and optical ambitious bridge, which combines different types of load carrying actions. The maximal span of the robust composite bridge is 132 m.
Der Lamellenstoß im Stahl‐ und Verbundbrückenbau
Authors: Kuhlmann Ulrike , Kudla Konrad 
Published: February 2013

AbstractAngeregt durch Erfahrungen von Ingenieurbüros und Stahlbaufirmen mit den neuen Ermüdungsnachweisen der europäischen Normen im Brückenbau, wurde im Rahmen eines DASt/IGF‐Forschungsvorhabens zur Weiterentwicklung und Spezifizierung der Ermüdungsnachweise für Straßen‐ und Eisenbahnbrücken unter anderem das Konstruktionsdetail des Lamellenstoßes untersucht. Dabei handelt es sich um einen typischen Montagestoß von Vollwandträgern aus Stahl mit mehrteiligen Gurten, der in Deutschland zwar übliche Baupraxis darstellt, aber im Ausland weitgehend unbekannt ist. Im Kerbfallkatalog von Eurocode 3 Teil 1‐9 zur Einordnung der Ermüdungsdetails fehlt deshalb der Lamellenstoß auch. Der Beitrag stellt erste Forschungsergebnisse vor, die es in Zukunft ermöglichen, auch bei Anwendung von Eurocode 3 das Konstruktionsdetail Lamellenstoß als Kerbfall einzuordnen und auszuführen.The lamella joint for steel and composite bridges, State of the Art in research and Eurocode design. Initiated by the experiences of consulting offices and steel companies applying the fatigue verification according to the new European rules in bridge design, in the frame of a DASt/IGF‐Research project dealing with the evolution and the specification of the fatigue assessment for highway and railway steel bridges the construction detail of a lamella joint has been investigated. This joint represents a typical butt welding joint during erection of plated steel girders with flanges consisting of several lamellas. This detail is typical of German bridge design in practice, but nearly unknown in other European countries. In the fatigue detail catalogue of Eurocode 3 Part 1‐9 the lamella joint is therefore missing. The paper presents first research results which will in future allow applying the construction detail of the lamella joint also in the frame of Eurocode design.
Die neue Rheinbrücke Wiesbaden‐Schierstein – Wettbewerb und Entwurf
Authors: Pelke Eberhard , Dieter Alwin 
Published: February 2013

AbstractOft unterschätzt, stand die bestehende Rheinbrücke Wiesbaden‐Schierstein am Wendepunkt vom statischen zum fertigungsgerechten Ingenieurbauwerk. Der Siegerentwurf des Realisierungswettbewerbes 2007 bestätigte die Grundlagen von Entwurf und Ausführung der Jahre 1959 bis 1962 und zieht Schlüsse aus der Unter‐ und baulichen Erhaltung des bestehenden Bauwerks. Der Aufsatz beschreibt Entstehung des bestehenden Bauwerks, wesentliche Instandsetzungsschritte, die sich daraus ableitende Erfordernis zum Neubau und abschließend dessen Ausschreibungsentwurf.The new Rhine Bridge Wiesbaden‐Schierstein, Germany – Competition and Design. The underestimated, existing Rhine Bridge Wiesbaden‐Schierstein was the watershed between static and erection optimization. The winner of the design competition in 2007 for a new Rhine Bridge between Wiesbaden and Mainz confirms the basic design and construction ideas of the former engineers. His design gathers experience of maintenance and retrofitting of the older bridge. The article describes the main steps of design, construction and retrofitting of the older bridge, need and tender design of a new bridge.
Development of an Empirically Based Questionnaire to Investigate Young Students' Ideas About Nature of Science
Authors: Chen Sufen , Chang Wen‐Hua , Lieu Sang‐Chong , Kao Huey‐Lien , Huang Mao‐Tsai , Lin Shu‐Fen 
Published: February 2013

AbstractThis study developed an empirically based questionnaire to monitor young learners' conceptions of nature of science (NOS). The questionnaire, entitled Students' Ideas about Nature of Science (SINOS), measured views on theory‐ladenness, use of creativity and imagination, tentativeness of scientific knowledge, durability of scientific knowledge, coherence and objectivity in science, the science for girls stereotype, and the science for boys stereotype. SINOS was constructed based on the written responses of 431 sixth graders, elementary students' quotations in theses and books related to NOS, and student interviews. The draft questionnaire was administered to 1,139 third to sixth graders (aged 9–12) in Taiwan. According to the results of an item analysis, and reliability and validity tests, 47 out of 62 items were selected for the current version. It was further validated by a sample of 1,091 5th and 6th graders. Confirmatory factor analysis revealed that all of the 5th graders, 6th graders, girls, and boys' data could be explained by the 7‐factor structure. The overall reliability as measured by Cronbach's alpha was 0.85. The relations between the SINOS scores and the achievement scores are presented. The instrument yields valid and reliable data useful for inferential statistics, and allows researchers and educators to relate NOS views to other measurable educational outcomes. © 2013 Wiley Periodicals, Inc. J Res Sci Teach 50: 408‐430, 2013
Calcium ion channels: challenges and successes in drug discovery
Authors: McDonough Stefan I. 
Published: February 2013

AbstractThere are both opportunities and challenges in developing improved or novel therapeutics based on targeting voltage‐gated calcium channels. Calcium channels are technically difficult with respect to target‐based drug discovery but unquestionably are key points for influencing human physiology and pathophysiology. Many marketed drugs, including nifedipine, nimodipine, ethosuximide, pregabalin, and ziconotide, exert therapeutic efficacy via inhibition of calcium channels. Although there is no obvious path to improving these existing drugs, recent clinical results with dihydropyridine drugs point to testable strategies for treatment of Parkinson's disease and possibly other CNS disorders by inhibiting L‐type calcium channels. Roles of T‐type calcium channels in pain and in abnormal sleep are plausible and may soon be tested with clinical trials of novel therapeutics. Success with any of these trials certainly will be followed by intensive efforts for further refinements around the target class. WIREs Membr Transp Signal 2013, 2:85–104. doi: 10.1002/wmts.71For further resources related to this article, please visit the WIREs website.
Image‐guided resection of malignant gliomas using ﬂuorescent nanoparticles
Authors: Su Xinhui , Cheng Kai , Wang Charlie , Xing Lei , Wu Hua , Cheng Zhen 
Published: February 2013

AbstractIntraoperative fluorescence imaging especially near‐infrared fluorescence (NIRF) imaging has the potential to revolutionize neurosurgery by providing high sensitivity and real‐time image guidance to surgeons for defining gliomas margins. Fluorescence probes including targeted nanoprobes are expected to improve the specificity and selectivity for intraoperative fluorescence or NIRF tumor imaging. The main focus of this article is to provide a brief overview of intraoperative fluorescence imaging systems and probes including fluorescein sodium, 5‐aminolevulinic acid, dye‐containing nanoparticles, and targeted NIRF nanoprobes for their applications in image‐guided resection of malignant gliomas. Moreover, photoacoustic imaging is a promising molecular imaging modality, and its potential applications for brain tumor imaging are also briefly discussed. WIREs Nanomed Nanobiotechnol 2013, 5:219–232. doi: 10.1002/wnan.1212This article is categorized under:

Diagnostic Tools > Diagnostic Nanodevices
Diagnostic Tools > In Vivo Nanodiagnostics and Imaging
Therapeutic Approaches and Drug Discovery > Nanomedicine for Neurological Disease
Therapeutic Approaches and Drug Discovery > Nanomedicine for Oncologic Disease
Improve carbon metabolic flux inSaccharomyces cerevisiaeat high temperature by overexpressedTSL1gene
Authors: Ge Xiang-Yang , Xu Yan , Chen Xiang 
Published: February 2013

AbstractThis study describes a novel strategy to improve the glycolysis flux of Saccharomyces cerevisiae at high temperature. The TSL1 gene-encoding regulatory subunit of the trehalose synthase complex was overexpressed in S. cerevisiae Z-06, which increased levels of trehalose synthase activity in extracts, enhanced stress tolerance and glucose consuming rate of the yeast cells. As a consequence, the final ethanol concentration of 185.5 g/L was obtained at 38 °C for 36 h (with productivity up to 5.2 g/L/h) in 7-L fermentor, and the ethanol productivity was 92.7 % higher than that of the parent strain. The results presented here provide a novel way to enhance the carbon metabolic flux at high temperature, which will be available for the purposes of producing other primary metabolites of commercial interest using S. cerevisiae as a host.
GATE Teamware: a web-based, collaborative text annotation framework
Authors: Bontcheva Kalina , Cunningham Hamish , Roberts Ian , Roberts Angus , Tablan Valentin , Aswani Niraj , Gorrell Genevieve 
Published: February 2013

Abstract
This paper presents GATE Teamware—an open-source, web-based, collaborative text annotation framework. It enables users to carry out complex corpus annotation projects, involving distributed annotator teams. Different user roles are provided (annotator, manager, administrator) with customisable user interface functionalities, in order to support the complex workflows and user interactions that occur in corpus annotation projects. Documents may be pre-processed automatically, so that human annotators can begin with text that has already been pre-annotated and thus making them more efficient. The user interface is simple to learn, aimed at non-experts, and runs in an ordinary web browser, without need of additional software installation. GATE Teamware has been evaluated through the creation of several gold standard corpora and internal projects, as well as through external evaluation in commercial and EU text annotation projects. It is available as on-demand service on GateCloud.net, as well as open-source for self-installation.
Risk Factors for Problem Gambling Among Indigenous Australians: An Empirical Study
Authors: Hing Nerilee , Breen Helen , Gordon Ashley , Russell Alex 
Published: February 2013

AbstractDespite a long history of gambling amongst many Indigenous peoples, knowledge about contemporary Indigenous gambling is sparse. In Australia, previous studies of Indigenous gambling have been severely limited in number, scope and rigour. The research reported in this paper is based on the first Indigenous-specific quantitative gambling research undertaken in Australia since 1996 and draws on the largest sample to date. This study examined numerous aspects of gambling among Indigenous Australians. After appropriate consultations and permission, the study collected surveys from 1,259 self-selected Indigenous adults in 2011 at three Indigenous festivals, online and in several Indigenous communities. This paper draws on these data to identify problem gambling risk factors by comparing selected socio-demographic characteristics, early exposure to gambling, gambling motivations, gambling behaviour, gambling cognitions, and substance use while gambling, amongst non-problem, low risk, moderate risk and problem gamblers. A logistic regression investigated the difference between problem gamblers and all other PGSI groups. Risk factors associated with being a problem gambler were: being older, commencing gambling when under 10 years old, always being exposed to adults gambling as a child, using alcohol and/or drugs while gambling, having family and friends who gamble, having an addiction to gambling and not gambling to socialise, having a high expenditure on commercial gambling, and living in a state or territory other than NSW or QLD. Public health measures to address these risk factors are identified.
Snow evens fragmentation effects and food determines overwintering success in ground‐dwelling voles
Authors: Haapakoski Marko , Ylönen Hannu 
Published: February 2013

AbstractClimate instability strongly affects overwintering conditions in organisms living in a strongly seasonal environment and consequently their survival and population dynamics. Food, predation and density effects are also strong during winter, but the effect of fragmentation of ground vegetation on ground‐dwelling small mammals is unknown. Here, we report the results of a winter experiment on the effects of habitat fragmentation and food on experimental overwintering populations of bank voles Myodes glareolus. The study was conducted in large outdoor enclosures containing one large, two medium‐sized or four small habitat patches or the total enclosure area covered with protective tall‐grass habitat. During the stable snow cover of midwinter, only food affected the overwintering success, body condition, trappability and earlier onset of breeding in bank voles. However, after the snow thaw in spring, habitat fragmentation gained importance again, and breeding activities increased the movements of voles in the most fragmented habitat. The use of an open, risky matrix area increased along the habitat fragmentation. Our experiment showed that long‐lasting stable snow cover protects overwintering individuals in otherwise exposed and risky ground habitats. We conclude that a stable winter climate and snow cover should even out habitat fragmentation effects on small mammals. However, along prolonged snow‐free early winter and in an earlier spring thaw, this means loss of protection by snow cover both in terms of thermoregulation and predation. Thus, habitat cover is important for the survival of small ground‐dwelling boreal mammals also during the non‐breeding season.
Inhibitory Effect of N‐Acyl Dopamines on IgE‐Mediated Allergic Response in RBL‐2H3 Cells
Authors: Yoo Jae‐Myung , Park Eun Seok , Kim Mee Ree , Sok Dai‐Eun 
Published: February 2013

AbstractRecently, endogenous N‐acyl dopamines have been found to show anti‐inflammatory and immunomodulatory activities. However, the effect of the N‐acyl dopamines on allergic responses was not reported. In this study, we investigated whether N‐acyl dopamines might inhibit immunoglobulin E‐mediated degranulation in RBL‐2H3 cells. When RBL‐2H3 cells were exposed to palmitoyl dopamine (NP‐DA), oleoyl dopamine (NO‐DA) or arachidonoyl dopamine (NA‐DA) at micromolar levels, all these compounds significantly inhibited the release of β‐hexosaminidase, a marker of degranulation, as well as tumor necrosis factor (TNF)‐α. In comparison, NP‐DA, potently suppressing the release of β‐hexosaminidase (IC50, 3.5 μM) and TNF‐α (IC50, 2.2 μM), was more potent than NO‐DA or NA‐DA. Additionally, NP‐DA markedly suppressed the formation of prostaglandin E2, prostaglandin D2 and leukotriene C4, corresponding to pro‐inflammatory lipid mediators in asthma. In the mechanistic analyses, where the effect of NP‐DA on the FcεRI cascade was examined, NP‐DA significantly inhibited the phosphorylation and expression of Syk, but not Lyn. And, NP‐DA also suppressed phosphorylation of ERK1/2 and Akt. Further, NP‐DA decreased the phosphorylation of cPLA2 and 5‐lipoxygenase (5‐LO), but not cyclooxygenase‐2 (COX‐2). Based on these results, it is suggested that NP‐DA exert anti‐allergic effect on allergic response through suppressing the activation of Syk, ERK1/2, Akt, cPLA2 and 5‐LO. Besides, a strong inhibition of COX‐2 activity by NP‐DA may be additional mechanism for its anti‐allergic action. Such an anti‐allergic action of N‐acyl dopamines may contribute to further information about biological functions of N‐acyl dopamines.