bluuebunny/crossref_metadata_2025_split huggingface dataset:
From 2D to 1D functionalization: Steps towards a carbon nanotube based biomembrane sensor for curvature sensitive proteins
Authors: Ostermaier Frieder , Scharfenberg Linda , Schneider Kristian , Hennig Stefan , Ostermann Kai , Posseckardt Juliane , Rödel Gerhard , Mertig Michael 
Published: February 2015

Membrane proteins are not homogeneously distributed throughout the membrane. One proposed localization mechanism is related to membrane curvature. In this work a sensor platform has been developed that uses carbon nanotubes to create curvature in a supported lipid bilayer. The specific binding of curvature sensitive proteins to the bent areas of the membrane is investigated by fluorescence microscopy. The setup will allow to study binding kinetics of curvature sensing motifs or to use such motifs for a one‐dimensional functionalization along the CNT.Fluorescence image of EGFP labeled curvature sensitive proteins that show linear alignment on a supported lipid bilayer above dielectrophoretically deposited single‐walled carbon nanotubes.
Toward a flexible and fine‐grained access control framework for infrastructure as a service clouds
Authors: Li Bo , Li Jianxin , Liu Lu , Zhou Chao 
Published: February 2015

AbstractCloud computing, as an emerging computing paradigm, greatly facilitates resource sharing and enables providing computing power as services over the Internet. However, it also brings new challenges for security and access control, especially in infrastructure as a service clouds. The introduction of virtualization layer increases new security risks, which should be restricted and confined by more stringent access control techniques. In this paper, we propose a flexible and fine‐grained access control framework, named IaaS‐oriented Hybrid Access Control (iHAC), which combines the advantages of both the role‐based access control and type enforcement model. We consider access control issues from the perspective of virtual machines. A permission transition model is designed to dynamically assign permissions to virtual machines. A Virtual Machine Monitor (VMM)‐based access control mechanism is presented to confine the virtual machine's behaviors in a fine‐grained manner. A VMM‐enabled network access control approach is proposed to regulate the communication among virtual machines. iHAC is successfully implemented in the Internet based Virtual Computing Infrastructure (iVIC) platform, and several experiments are conducted to evaluate its effectiveness and efficiency. The results show that iHAC can make correct access control decisions with low performance overhead. Copyright © 2015 John Wiley & Sons, Ltd.
Modeling and verification of trust and reputation systems
Authors: Aldini Alessandro 
Published: February 2015

AbstractTrust is a basic soft‐security condition influencing interactive and cooperative behaviors in online communities. Several systems and models have been proposed to enforce and investigate the role of trust in the process of favoring successful cooperations while minimizing selfishness and failure. However, the analysis of their effectiveness and efficiency is a challenging issue. This paper provides a formal approach to the design and verification of trust infrastructures used in the setting of software architectures and computer networks supporting online communities. The proposed framework encompasses a process calculus of concurrent systems, a temporal logic for trust, and model checking techniques. Both functional and quantitative aspects can be modeled and analyzed, while several types of trust models can be integrated. Copyright © 2015 John Wiley & Sons, Ltd.
Identification of a non-redundant set of 202 in silico SSR markers and applicability of a select set in chickpea (Cicer arietinum L.)
Authors: Agarwal Gaurav , Sabbavarapu Murali M. , Singh Vikas K. , Thudi Mahendar , Sheelamary S. , Gaur Pooran M. , Varshney Rajeev K. 
Published: February 2015

Abstract The paucity of sequence information flanking the simple sequence repeat (SSR) motifs identified especially in the transcript sequences has been limiting factor in the development of SSR markers for plant genome analysis as well as breeding applications. To overcome this and enhance the genic SSR marker repertoire in chickpea, the draft genome sequence of kabuli chickpea (CDC Frontier) and publicly available transcript sequences consisting of in silico identified SSR motifs were deployed in the present study. In this direction, the 300 bp sequence flanking the SSR motifs were retrieved by aligning 566 SSR containing transcripts of ICCV 2 available in public domain on the reference chickpea genome. A set of 202 novel genic SSRs were developed from a set of 507 primer pairs designed, based on in silico amplification of single locus and having no similarity to the publicly available SSR markers. Further, 40 genic SSRs equally distributed on chickpea genome were validated on a select set of 44 chickpea genotypes (including 41 Cicer arietinum and 3 Cicer reticulatum), out of which 25 were reported to be polymorphic. The polymorphism information content (PIC) value of 25 polymorphic genic SSRs ranged from 0.11 to 0.77 and number of alleles varied from 2 to 9. Clear demarcation among founder lines of multi-parent advanced generation inter-cross (MAGIC) population developed at ICRISAT and near-isogenic nature of JG 11 and JG11 + demonstrates the usefulness of these markers in chickpea diversity analysis and breeding studies. Further, genic polymorphic SSRs reported between parental lines of 16 different mapping populations along with the novel SSRs can be deployed for trait mapping and breeding applications in chickpea.
Analysis of Phosphoinositide‐Binding Properties and Subcellular Localization of GFP‐Fusion Proteins
Authors: Jun Yong‐Woo , Kim Sangyeol , Kim Kun‐Hyung , Lee Jin‐A , Lim Chae‐Seok , Chang Iksoo , Suh Byung‐Chang , Kaang Bong‐Kiun , Jang Deok‐Jin 
Published: February 2015

AbstractSpecific protein‐phosphoinositide (PI) interactions are known to play a key role in the targeting of proteins to specific cellular membranes. Investigation of these interactions would be greatly facilitated if GFP‐fusion proteins expressed in mammalian cells and used for their subcellular localization could also be employed for in vitro lipid binding. In this study, we found that lysates of cells overexpressing GFP‐fusion proteins could be used for in vitro protein‐PI binding assays. We applied this approach to examine the PI‐binding properties of Aplysia Sec7 protein (ApSec7) and its isoform ApSec7(VPKIS), in which a VPKIS sequence is inserted into the PH domain of ApSec7. EGFP‐ApSec7 but not EGFP‐ApSec7(VPKIS) did specifically bind to PI(3,4,5)P3 in an in vitro lipid‐coated bead assay. Overexpression of EGFP‐ApSec7 but not EGFP‐ApSec7(VPKIS) did induce neurite outgrowth in Aplysia sensory neurons. Structure modeling analysis revealed that the inserted VPKIS caused misfolding around the PI(3,4,5)P3‐binding pocket of ApSec7 and disturbed the binding of PI(3,4,5)P3 to the pleckstrin homology (PH) domain. Our data indicate that plasma membrane localization of EGFP‐ApSec7 via the interaction between its PH domain and PI(3,4,5)P3 might play a key role in neurite outgrowth in Aplysia.
Prevalence of helminth infestation during pregnancy and its association with maternal anemia and low birth weight
Authors: Aderoba Adeniyi K. , Iribhogbe Oseihie I. , Olagbuji Biodun N. , Olokor Oghenefegor E. , Ojide Chiedozie K. , Ande Adedapo B. 
Published: February 2015

AbstractObjectiveTo determine the prevalence of helminth infestation during pregnancy and the associated risks of adverse maternal and infant outcomes.MethodsA cross‐sectional study of women with a singleton pregnancy of at least 34 weeks was conducted at a teaching hospital in Benin City, Nigeria, between April 1 and September 30, 2010. Socioeconomic and clinical data were obtained. Stool samples were used to determine helminth infection. Birth weight was recorded at delivery. Multivariable analysis was used to assess the link between helminth infestation and maternal and perinatal outcomes.ResultsAmong 178 women, 31 (17.4%) had a helminth infestation (15 [8.4%] had ascariasis, 8 [4.5%] trichuriasis, and 25 [14.0%] hookworm infestation). Multivariate analysis found that helminth infestations was associated with maternal anemia (adjusted odds ratio 12.4; 95% confidence interval 4.2–36.3) and low birth weight (adjusted odds ratio 6.8; 95% confidence interval 2.1–21.9).ConclusionApproximately one in five women had a helminth infestation in the third trimester of pregnancy. Maternal helminth infestation significantly increased the risks of maternal anemia and low birth weight, indicating that routine administration of anthelminthic drugs during early pregnancy might improve perinatal outcomes.
Reconstruction of ancestral drainage patterns in an internally draining region, Fars Province, Iran
Authors: LEE JACQUELINE 
Published: February 2015

AbstractGoogle Earth imagery is used here in conjunction with a Geographic Information System to identify transverse drainages and recreate palaeodrainage in an internally draining region in Iran. At least 35 water gaps and 34 wind gaps are shown to exist in the region, as well as evidence for an integrated palaeodrainage that originated north of the internally draining region and emptied into the Mand watershed to the west. The topographic characteristics of the transverse drainages suggest a strong control by local topography, and support formation of the internally draining region by basin filling and overflow. Both climatic and tectonic factors may have controlled the loss of external connectivity.
A systematic review on empowerment for healthy nutrition in health promotion
Authors: Brandstetter Susanne , Rüter Jana , Curbach Janina , Loss Julika 
Published: February 2015

AbstractObjectiveThe present review aimed to identify and synthesize studies that used an empowerment approach within the field of healthy nutrition.DesignA systematic review was conducted. Studies were identified by database searching (PubMed, PsycINFO, Web of Science and Psyndex). Searching, selecting and reporting were done according to the PRISMA (Preferred Reporting Items for Systematic reviews and Meta-Analyses) Statement.SettingHealth promotion including the subject of healthy nutrition.SubjectsIndividuals from non-clinical populations.ResultsA total of 1226 studies were screened for eligibility, eight studies were finally included. Three studies used the empowerment approach within a qualitative research paradigm and five studies within (quasi-) experimental intervention studies. Heterogeneity in settings, samples and evaluation methods was high. Most studies referred to the key message of empowerment, i.e. taking control over one’s own life. However, the ways in which this key message was implemented in the interventions differed considerably.ConclusionsThe number of studies included was very low. Furthermore, most studies had some limitations in terms of reporting how the empowerment approach was actually applied. The empowerment approach still seems to be unfamiliar within the field of healthy nutrition.
Expression profiling of the bloom-forming cyanobacterium Nodularia CCY9414 under light and oxidative stress conditions
Authors: Kopf Matthias , Möke Fred , Bauwe Hermann , Hess Wolfgang R , Hagemann Martin 
Published: February 2015

Abstract
Massive blooms of toxic cyanobacteria frequently occur in the central Baltic Sea during the summer. In the surface scum, cyanobacterial cells are exposed to high light (HL) intensity, high oxygen partial pressure and other stresses. To mimic these conditions, cultures of Nodularia spumigena CCY9414, which is a strain isolated from a cyanobacterial summer bloom in the Baltic Sea, were incubated at a HL intensity of 1200 μmol photons m−2 s−1 or a combination of HL and increased oxygen partial pressure. Using differential RNA sequencing, we compared the global primary transcriptomes of control and stressed cells. The combination of oxidative and light stresses induced the expression of twofold more genes compared with HL stress alone. In addition to the induction of known stress-responsive genes, such as psbA, ocp and sodB, Nodularia cells activated the expression of genes coding for many previously unknown light- and oxidative stress-related proteins. In addition, the expression of non-protein-coding RNAs was found to be stimulated by these stresses. Among them was an antisense RNA to the phycocyanin-encoding mRNA cpcBAC and the trans-encoded regulator of photosystem I, PsrR1. The large genome capacity allowed Nodularia to harbor more copies of stress-relevant genes such as psbA and small chlorophyll-binding protein genes, combined with the coordinated induction of these and many additional genes for stress acclimation. Our data provide a first insight on how N. spumigena became adapted to conditions relevant for a cyanobacterial bloom in the Baltic Sea.
Insights into the Role of Substrates on the Interaction between Cytochrome b5 and Cytochrome P450 2B4 by NMR
Authors: Zhang Meng , Le Clair Stéphanie V. , Huang Rui , Ahuja Shivani , Im Sang-Choul , Waskell Lucy , Ramamoorthy Ayyalusamy 
Published: February 2015

AbstractMammalian cytochrome b5 (cyt b5) is a membrane-bound protein capable of donating an electron to cytochrome P450 (P450) in the P450 catalytic cycle. The interaction between cyt b5 and P450 has been reported to be affected by the substrates of P450; however, the mechanism of substrate modulation on the cyt b5-P450 complex formation is still unknown. In this study, the complexes between full-length rabbit cyt b5 and full-length substrate-free/substrate-bound cytochrome P450 2B4 (CYP2B4) are investigated using NMR techniques. Our findings reveal that the population of complexes is ionic strength dependent, implying the importance of electrostatic interactions in the complex formation process. The observation that the cyt b5-substrate-bound CYP2B4 complex shows a weaker dependence on ionic strength than the cyt b5-substrate-free CYP2B4 complex suggests the presence of a larger fraction of steoreospecific complexes when CYP2B4 is substrate-bound. These results suggest that a CYP2B4 substrate likely promotes specific interactions between cyt b5 and CYP2B4. Residues D65, V66, T70, D71 and A72 are found to be involved in specific interactions between the two proteins due to their weak response to ionic strength change. These findings provide insights into the mechanism underlying substrate modulation on the cyt b5-P450 complexation process.
Epitaxial graphene contact electrode for silicon carbide based ultraviolet photodetector
Authors: Kus¸demir Erdi , Özkendir Dilce , Fırat Volkan , Çelebi Cem 
Published: February 2015

Abstract
We present the fabrication and characterization of graphene-semiconductor-graphene ultraviolet photodetector based on the rectifying character of Schottky junction at the interface between epitaxial graphene and SiC semiconductor. As-grown single layer epitaxial graphene is interdigitated as transparent conductive electrode to probe photo-generated charge carriers in a semi-insulating 4H-SiC substrate. The fabricated device exhibits the typical current-voltage characteristics of a conventional metal-semiconductor-metal type photodetector with low leakage current. Time-resolved photocurrent measurements suggest an excellent photocurrent reversibility and high response speed of the device. The measurements performed for different illumination wavelengths showed that the sample reveals higher responsivity values when it is exposed to the light with 254 nm wavelength. The obtained results imply that epitaxial graphene can be used readily as transparent conductive electrode for SiC based optoelectronic device applications.
Exploration of the critical depth hypothesis with a simple NPZ model
Authors: Lévy Marina 
Published: February 2015

AbstractThe critical depth hypothesis (CDH) is a predictive criteria for the onset of phytoplankton blooms that comes from the steady-state analytical solution of a simple mathematical model for phytoplankton growth presented by Sverdrup in 1953. Sverdrup's phytoplankton-only model is very elementary compared with state-of-the-art ecosystem models whose numerical solution in a time-varying environment do not systematically conform to the CDH. To highlight which model ingredients make the bloom onset deviate from the CDH, the complexity of Sverdrup's model is incrementally increased, and the impact that each new level of complexity introduced is analysed. Complexity is added both to the ecosystem model and to the parameterization of physical forcing. In the most complete experiment, the model is a one-dimensional Nutrient-Phytoplankton-Zooplankton model that includes seasonally varying mixed layer depth and surface irradiance, light and nutrient limitation, variable grazing, self-shading, export, and remineralization. When complexity is added to the ecosystem model, it is found that the model solution only marginally deviates from the CDH. But when the physical forcing is also changed, the model solution can conform to two competing theories for the onset of phytoplankton blooms—the critical turbulence hypothesis and the disturbance recovery hypothesis. The key roles of three physical ingredients on the bloom onset are highlighted: the intensity of vertical mixing at the end of winter, the seasonal evolution of the mixed-layer depth from the previous summer, and the seasonal evolution of surface irradiance.
Custom executive education program charters: a beneficial task and useful template
Authors: Haskins Mark E. , Clawson James G. 
Published: February 2015

Purpose
– This paper introduces readers to the usefulness of, design of, and an example of a custom executive education program charter. As such, charters are posed as a key task to complete at the outset of a new custom executive education provider/client relationship.

Design/methodology/approach
– This paper codifies the attributes of a well-conceived and well-crafted program charter based on a number of years of having led custom executive education program design, development, and delivery activities.

Findings
– Six components of a useful and substantive custom executive education program charter are identified. Each is discussed as are the roles that a completed charter can contribute to in an ongoing custom executive education provider/client relationship.

Practical implications
– The field-inspired custom executive education program charter insights presented are immediately actionable by program providers and/or the client sponsors of such programs.

Originality/value
– Readers are provided with a template for crafting their own charters. The merits of having a charter are highlighted, as are some of the challenges involved in crafting one.
As a leader are you trustworthy? Building trust to transform team working
Authors: Sabatier Marielena 
Published: February 2015

Purpose
– The purpose of this article is to highlight the importance, from the author’s experience, of improving team working and rebuilding trust. The issue of trust is high on the agenda for many organizations, especially following the financial crisis and recent scandals that have affected sectors such as banking, retail and energy in the UK. Executive Coach Marielena Sabatier, CEO, Inspiring Potential examines why trust is so important in organizations and what business leaders can do to build trust amongst their teams and embed a culture of trust.

Design/methodology/approach
– The article takes a practical approach focusing on why trust has been damaged in organizations in recent years, why trust is so important and how senior mangers can build trust, providing tips on how to develop a culture of trust.

Findings
– Key findings are organizations that want to have a culture of trust must first analyse where they are going wrong, then leaders need to communicate a clear mission and purpose for the business and employees, they must lead by example and act with integrity always. Other key ingredients are to right any wrongs, to hold people accountable and to communicate openly at all times.

Originality/value
– The article highlights the importance, from the author’s experience, of improving team working and rebuilding trust.
The impact of social media on L&D: and what we should be doing about it
Authors: Paine Nigel 
Published: February 2015

Purpose
– This article aims to provide a succinct summary of the actions learning and development (L&D) professionals should take to get involved in and make the most of social media, both for themselves and their organizations.

Design/methodology/approach
– This viewpoint is based on the author’s book published in 2014, which explores how corporate learning has changed in the past five years and what the big trends are currently.

Findings
– Social media is here to stay and will continually reinvent itself at perhaps even greater speed. L&D professionals in particular must quickly learn to embrace the technology to assist learners to build a personal knowledge framework and thereby assure their usefulness and enduring competence.

Originality/value
– By embracing social media you create an enabling and facilitating model, which shifts the burden of responsibility firmly onto the learner.
The art of influence: apply emotional intelligence and create time and space for thinking
Authors: Nabben Jennifer 
Published: February 2015

Purpose
– This paper aims to explore how emotional intelligence and the emerging field of neuroscience can tell us much about how leaders can use their own emotions to positively influence people. The ability for leaders to positively influence others is a critical skill and yet it is often identified as a gap in leadership teams.

Design/methodology/approach
– The paper draws on the work of Goleman, Kline, Ramachandran and Schwartz, as well as the author’s own recently published book.

Findings
– An emotionally intelligent leader, rather than assuming that those who speak the loudest have the most valuable contribution will be more likely to understand and value different thinking and personality styles and will take active steps to create an environment where people feel safe to contribute their very best thinking.

Practical implications
– This paper provides useful, practical advice to those involved in leadership development or leaders themselves who want to better understand how they can improve their ability to influence others.

Originality/value
– The paper provides practical advice, grounded in research, that professional learning and development executives or leaders themselves can put in action.