bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of Attention in Placebo and Nocebo Effects
Authors: Barbiani Diletta , Camerone Eleonora M , Grosso Francesca , Geers Andrew L , Pagnini Francesco 
Published: July 2024

Abstract

Background
Although some existing models propose that attention may be crucially implicated in placebo/nocebo effects, empirical research on this aspect remains limited and scattered.

Purpose
This systematic review aims to provide an inclusive overview of studies that have either directly manipulated or assessed attention within the context of placebo and nocebo procedures so to gain a synthetized picture of the role of this variable in placebo/nocebo effects. Importantly, only studies in which attention represented a mechanism or mediator of the placebo/nocebo response, and not a primary outcome, were included.

Methods
A systematic search was conducted across multiple databases, including PubMed, Scopus, PsycINFO, Web of Science, and Embase, to identify peer-reviewed studies. These studies were subjected to methodological evaluation and eligibility criteria for inclusion.

Results
We identified and classified 12 studies into three categories based on their focus: (i) those that directly assessed attention, (ii) those that directly manipulated participants’ attention, and (iii) those that combined both a direct manipulation and assessment of attention. In all selected studies attention acted as a mechanism or mediator of the placebo/nocebo response, and was not considered a primary outcome of the placebo/nocebo manipulation.

Conclusions
The synthesis of the included studies reveals that the role of attention in placebo and nocebo effects is still a topic of debate, marked by variations in how attention is conceptualized and measured. Results suggest that attention has significant clinical implications, particularly in optimizing therapeutic efficacy by directing patients’ focus toward signs of healing and away from indicators of illness or distress. To advance our understanding, future research should explore these attentional mechanisms, in conjunction with neurophysiological correlates.
Prospective transitions in hemoglobin A1c following gestational diabetes using multistate Markov models
Authors: McCarthy Katharine J , Liu Shelley H , Kennedy Joseph , Chan Hiu Tai , Mayer Victoria L , Vieira Luciana , Glazer Kimberly B , Van Wye Gretchen , Janevic Teresa 
Published: July 2024

Abstract
We characterized the state-to-state transitions in postpartum hemoglobin A1c levels after gestational diabetes, including remaining in a state of normoglycemia or transitions between prediabetes or diabetes states of varying severity. We used data from the APPLE Cohort, a postpartum population-based cohort of individuals with gestational diabetes between 2009 and 2011, and linked A1c data with up to 9 years of follow-up (n = 34 171). We examined maternal sociodemographic and perinatal characteristics as predictors of transitions in A1c progression using Markov multistate models. In the first year postpartum following gestational diabetes, 45.1% of people had no diabetes, 43.1% had prediabetes, 4.6% had controlled diabetes, and 7.2% had uncontrolled diabetes. Roughly two-thirds of individuals remained in the same state in the next year. Black individuals were more likely to transition from prediabetes to uncontrolled diabetes (adjusted hazard ratio [aHR] = 2.32; 95% CI, 1.21-4.47) than White persons. Perinatal risk factors were associated with disease progression and a lower likelihood of improvement. For example, hypertensive disorders of pregnancy were associated with a stronger transition (aHR = 2.06; 95% CI, 1.39-3.05) from prediabetes to uncontrolled diabetes. We illustrate factors associated with adverse transitions in incremental A1c stages and describe patient profiles that may warrant enhanced postpartum monitoring.
Impact of a new state law and electronic health record prompt on naloxone prescribing in an academic medical center
Authors: Dugovich Anna , Robert Sophie , Hatton James , Jewett Amanda 
Published: July 2024

Abstract

Purpose
To evaluate the impact of a best-practice advisory (BPA) and South Carolina legislation on naloxone prescribing patterns. The primary objective was to assess the change in naloxone prescription rates following BPA implementation. The secondary objective was to analyze the performance of the BPA.

Methods
Naloxone prescriptions generated before (July 28, 2020, through July 27, 2021) and after (July 28, 2021, through July 28, 2022) BPA implementation were analyzed via retrospective chart review. Lists of patients at risk for opioid overdose and patients for whom the BPA fired were generated for March 2022. The BPA’s effectiveness was evaluated based on the proportion of at-risk patients missed by the alert, the frequency with which the BPA resulted in a naloxone prescription, and the reasons for not prescribing naloxone when the BPA fired.

Results
Following BPA implementation, there was a significant increase in the average monthly naloxone prescribing rate from 66.1 to 625.5 prescriptions per month. Overall, 2,086 patients were considered at risk for opioid overdose and 1,101 had a BPA alert during March 2022, with 32.7% of BPA alerts resulting in naloxone prescribing. The most common reasons selected for not prescribing naloxone were “patient refusal” and “criteria not met.” Only 354 patients (17.1%) at risk for opioid overdose also had a BPA alert.

Conclusion
State legislation and implementation of the BPA significantly increased naloxone prescribing rates. However, a significant proportion of patients identified as being at risk did not have a BPA alert and most BPA alerts did not result in naloxone prescribing, suggesting a need for improvement of the BPA.
Positive pressure in bamboo is generated in stems and rhizomes, not in roots
Authors: Michaud Joseph M , Mocko Kerri , Schenk H Jochen 
Published: July 2024

Abstract
Bamboos stand out among other tall plants in being able to generate positive pressure in the xylem at night, pushing water up to the leaves and causing drops to fall from leaf tips as guttation that can amount to a steady nocturnal ‘bamboo rain’. The location and mechanism of nocturnal pressure generation in bamboos are unknown, as are the benefits for the plants. We conducted a study on the tall tropical bamboo species Bambusa oldhamii (giant timber bamboo) growing outdoors in southern California under full irrigation to determine where in the plant the nocturnal pressure is generated, when it rises in the evening, and when it dissipates in the morning. We hypothesized that the build-up of positive pressure would be triggered by the cessation of transpiration-driven sap flow and that resumption of sap flow in the morning would cause the pressure to dissipate. Nocturnal pressure was observed in mature stems and rhizomes, but never in roots. The pressure was episodic and associated with stem swelling and was usually, but not always, higher in rhizomes and basal stems than in stems at greater height. Time series analyses revealed that dry atmospheric conditions were followed by lower nocturnal pressure and rainfall events by higher stem pressure. Nocturnal pressure was unrelated to sap flow and even was generated for a short time in isolated stem pieces placed in water. We conclude that nocturnal pressure in bamboo is not ‘root pressure’ but is generated in the pseudo-woody rhizomes and stems. It is unrelated to the presence or absence of sap flow and therefore must be created outside of vessels, such as in phloem, parenchyma, or fibres. It is unlikely to be a drought adaptation and may benefit the plants by maximizing stem water storage for daytime transpiration or by transporting nutrients to the leaves.
A Bibliometric Analysis of the 100 Most-Cited Articles on Otoplasty
Authors: Arif Fizzah , Rehman Saif ur , Shahzad Zenab , Rahman Mohammad Fazlur , Shaikh Safdar Ali 
Published: July 2024

Abstract

A bibliometric analysis was conducted in April 2024 to review the current trends in otoplasty. It involved a literature search of the Scopus database for original articles with the query terms “otoplasty” and “pinnaplasty,” without restricting publication dates or selecting journals in the database. The top 100 articles with the highest citations were reviewed. Bibliometric analysis was performed with the Scimago journal impact factor. The screening was done following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines to extract the top 100 most-cited articles in otoplasty. We excluded articles not focused on otoplasty, those involving other surgical procedures, and non-English articles. With Scopus and manual screening, we performed citation analysis of selected articles. Mean values were calculated for the number of citations and citations per year. Of the 951 studies identified, 100 (10.5%) were analyzed in detail. The studies were published between 1941 and 2024, with a mean of 36 ± 23.7 citations per paper. The journal Plastic and Reconstructive Surgery reported the highest number (23%, n = 23) of articles, followed by the Journal of Plastic, Reconstructive and Aesthetic Surgery with 13% (n = 13) articles. The highest number of articles originated from the United States (38%, n = 38), followed by the United Kingdom (15%, n = 15). Our bibliometric analysis provides a comprehensive overview of the landscape of otoplasty research, highlighting key publications, authors, and journals. This study contributes to the understanding of the evolution and impact of otoplasty literature, laying the groundwork for further research and innovation in this field.

Level of Evidence: 3
Managing Trouble Along a Continuum of Accountability: On Police Practices for Dealing with Less-Than-Competent Subjects
Authors: Buscariolli André 
Published: July 2024

Abstract
This article uses conversation analysis to examine recordings of police encounters, focusing on how officers respond to routine forms of trouble emerging from civilians’ failure to comprehend and cooperate with their initiating actions. The analysis demonstrates how officers improvise methods to deal with their subjects’ troublesome responses to their queries and directives, treating civilians along a continuum from being purposefully uncooperative to lacking the interactional competence to comply with their projects. In the latter case, officers treat noncompliance as related to one’s status as “mentally disordered,” adopting practices to progressively carve out islands of competence within which civilians are competent to cooperate or accountable for not doing so. Thus, officers’ orientation to a continuum of accountability shapes the encounter’s interactional trajectory toward more punitive or instructive outcomes.
COVID-19 and the New Pains of Imprisonment
Authors: Horowitz Veronica L , Andersen Synøve N , Hyatt Jordan M 
Published: July 2024

Abstract
As the COVID-19 pandemic upended life worldwide, prisons gained attention as epicentres for the virus. The focus was primarily on infections and death rates, often omitting the impact on incarcerated people. This study draws on semi-structured interviews (n = 58) with men imprisoned throughout the pandemic. Using and extending classic and contemporary theorizations of ‘the pains of imprisonment’, we find that official pandemic responses meant that (1) new, pandemic-related pains developed, (2) established pains changed in severity and took new manifestations, and (3) pains were experienced simultaneously and interactively. Thus, the pandemic amplified, diversified and compounded the pains of imprisonment. While most visible in the context of a large-scale crisis, these concepts provide an expanded, broadly applicable framing for future carceral scholarship.
Chondroitin sulfate proteoglycan 4 increases invasion of recessive dystrophic epidermolysis bullosa-associated cutaneous squamous cell carcinoma by modifying transforming growth factor-β signalling
Authors: Macaulay Allison R K , Yang Jianbo , Price Matthew A , Forster Colleen L , Riddle Megan J , Ebens Christen L , Albert Frank W , Giubellino Alessio , McCarthy James B , Tolar Jakub 
Published: July 2024

Abstract

Background
Recessive dystrophic epidermolysis bullosa (RDEB) is a rare genetic skin-blistering disorder that often progresses to metastatic cutaneous squamous cell carcinoma (cSCC) at chronic wound sites. Chondroitin sulfate proteoglycan 4 (CSPG4) is a cell-surface proteoglycan that is an oncoantigen in multiple malignancies, where it modulates oncogenic signalling, drives epithelial-to-mesenchymal transition (EMT) and enables cell motility.

Objectives
To evaluate CSPG4 expression and function in RDEB cSCC.

Methods
RDEB cSCC cell lines were used to assess CSPG4-dependent changes in invasive potential, transforming growth factor (TGF)-β1-stimulated signal activation and clinically relevant cytopathology metrics in an in vitro full-thickness tumour model. CSPG4 expression in RDEB cSCC and non-RDEB cSCC tumours was analysed via immunohistochemistry and single-cell RNA sequencing (scRNA-Seq), respectively.

Results
Inhibiting CSPG4 expression reduced invasive potential in multiple RDEB cSCC cell lines and altered membrane-proximal TGF-β signal activation via changes in SMAD3 phosphorylation. CSPG4 expression was uniformly localized to basal layer keratinocytes in fibrotic RDEB skin and tumour cells at the tumour–stroma interface at the invasive front in RDEB cSCC tumours in vivo. Analysis of published scRNA-Seq data revealed that CSPG4 expression was correlated with an enhanced EMT transcriptomic signature in cells at the tumour–stroma interface of non-RDEB cSCC tumours. Cytopathological metrics, for example nucleus : cell area ratio, were influenced by CSPG4 expression in in vitro tumour models.

Conclusions
We determined that CSPG4 expression in RDEB cSCC cell lines enhanced the invasive potential of tumours. Mechanistically, CSPG4 was found to enhance membrane-proximal TGF-β-stimulated signalling via SMAD3, which is a key mediator of EMT in RDEB cSCC. The implication of these studies is that CSPG4 may represent a therapeutic target that can be leveraged for the clinical management of patients with RDEB cSCC.
Reduction of sacsin levels in peripheral blood mononuclear cells as a diagnostic tool for spastic ataxia of Charlevoix–Saguenay
Authors: De Ritis Daniele , Ferrè Laura , De Winter Jonathan , Tremblay-Desbiens Clémence , Blais Mathieu , Bassi Maria Teresa , Dupré Nicolas , Baets Jonathan , Filippi Massimo , Maltecca Francesca 
Published: July 2024

Abstract
Autosomal recessive spastic ataxia of Charlevoix–Saguenay is a rare neurodegenerative disease caused by biallelic variants in the SACS gene encoding for sacsin. More than 200 pathogenic variants have been identified to date, most of which are missense. It is likely that the prevalence of autosomal recessive spastic ataxia of Charlevoix–Saguenay is underestimated due to the lack of an efficient diagnostic tool able to validate variants of uncertain significance. We have previously shown that sacsin is almost absent in fibroblasts of patients with autosomal recessive spastic ataxia of Charlevoix–Saguenay regardless of the type of SACS variant, because sacsin carrying missense variants is cotranslationally degraded. In this work, we aimed to establish the pathogenicity of SACS variants by quantifying sacsin protein in blood samples, with relevant implications for autosomal recessive spastic ataxia of Charlevoix–Saguenay diagnosis. We developed a protocol to assess sacsin protein levels by western blot using small amounts of peripheral blood mononuclear cells, which can be propagated in culture and cryopreserved. The study involves eight patients with autosomal recessive spastic ataxia of Charlevoix–Saguenay (including a novel case) carrying variants of different types and positions along the SACS gene and two parents who are carriers of heterozygous missense variants. We show that patients with autosomal recessive spastic ataxia of Charlevoix–Saguenay (carrying either missense or truncating variants) almost completely lacked sacsin in peripheral blood mononuclear cells. Moreover, both carriers of a SACS missense variant showed 50% reduction in sacsin protein levels compared to controls. We also describe a patient with uniparental isodisomy carrying a homozygous nonsense variant near the 3′ end of the SACS gene. This resulted in a stable sacsin protein lacking the last 202 amino acids, probably due to escape of nonsense-mediated decay of mRNA. In conclusion, we have optimized a minimally invasive diagnostic tool for autosomal recessive spastic ataxia of Charlevoix–Saguenay in blood samples based on sacsin protein level assessment. Indeed, our results provide definite evidence that sacsin carrying missense pathogenic variants undergoes cotranslational degradation. The quantitative reduction in sacsin levels in the case of missense variants of uncertain significance allows defining them as pathogenic variants, something which cannot be predicted bioinformatically with high certainty.
Human genetic and immunological determinants of SARS-CoV-2 infection and multisystem inflammatory syndrome in children
Authors: Kholaiq Halima , Abdelmoumen Yousra , Moundir Abderrahmane , El Kettani Assiya , Ailal Fatima , Benhsaien Ibtihal , Adnane Fatima , Drissi Bourhanbour Asmaa , Amenzoui Naima , El Bakkouri Jalila , Bousfiha Ahmed Aziz 
Published: July 2024

Summary
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces pneumonia and acute respiratory failure in coronavirus disease 2019 (COVID-19) patients with inborn errors of immunity to type I interferon (IFN-I). The impact of SARS-CoV-2 infection varies widely, ranging from mild respiratory symptoms to life-threatening illness and organ failure, with a higher incidence in men than in women. Approximately 3–5% of critical COVID-19 patients under 60 and a smaller percentage of elderly patients exhibit genetic defects in IFN-I production, including X-chromosome-linked TLR7 and autosomal TLR3 deficiencies. Around 15–20% of cases over 70 years old, and a smaller percentage of younger patients, present with preexisting autoantibodies neutralizing type I interferons. Additionally, innate errors affecting the control of the response to type I interferon have been associated with pediatric multisystem inflammatory syndrome (MIS-C). Several studies have described rare errors of immunity, such as XIAP deficiency, CYBB, SOCS1, OAS1/2, and RNASEL, as underlying factors in MIS-C susceptibility. However, further investigations in expanded patient cohorts are needed to validate these findings and pave the way for new genetic approaches to MIS-C. This review aims to present recent evidence from the scientific literature on genetic and immunological abnormalities predisposing individuals to critical SARS-CoV-2 infection through IFN-I. We will also discuss multisystem inflammatory syndrome in children (MIS-C). Understanding the immunological mechanisms and pathogenesis of severe COVID-19 may inform personalized patient care and population protection strategies against future serious viral infections.
Distal but not local auditory information supports spatial representations by place cells
Authors: Dolón Vera Laura , Dietz Birte , Manahan-Vaughan Denise 
Published: July 2024

Abstract
Sound is an important navigational cue for mammals. During spatial navigation, hippocampal place cells encode spatial representations of the environment based on visual information, but to what extent audiospatial information can enable reliable place cell mapping is largely unknown. We assessed this by recording from CA1 place cells in the dark, under circumstances where reliable visual, tactile, or olfactory information was unavailable. Male rats were exposed to auditory cues of different frequencies that were delivered from local or distal spatial locations. We observed that distal, but not local cue presentation, enables and supports stable place fields, regardless of the sound frequency used. Our data suggest that a context dependency exists regarding the relevance of auditory information for place field mapping: whereas locally available auditory cues do not serve as a salient spatial basis for the anchoring of place fields, auditory cue localization supports spatial representations by place cells when available in the form of distal information. Furthermore, our results demonstrate that CA1 neurons can effectively use auditory stimuli to generate place fields, and that hippocampal pyramidal neurons are not solely dependent on visual cues for the generation of place field representations based on allocentric reference frames.
Prevalence, Clinical Severity, and Serotype Distribution of Pneumococcal Pneumonia Among Adults Hospitalized With Community-Acquired Pneumonia in Tennessee and Georgia, 2018–2022
Authors: Self Wesley H , Johnson Kelly D , Resser J Jackson , Whitney Cynthia G , Baughman Adrienne , Kio Mai , Grijalva Carlos G , Traenkner Jessica , Johnson Jakea , Miller Karen F , Rostad Christina A , Yildirim Inci , Salazar Luis , Tanios Ralph , Swan Sydney A , Zhu Yuwei , Han Jin H , Weiss Thomas , Roberts Craig , Rouphael Nadine ,   For the PNEUMO Study Investigators, Self Wesley H , Resser J Jackson , Baughman Adrienne , Grijalva Carlos G , Johnson Jakea , Miller Karen F , Swan Sydney A , Zhu Yuwei , Han Jin H , Shipman Sabrina , Rouphael Nadine , Whitney Cynthia , Kio Mai , Traenkner Jessica , Rostad Christina , Yildirim Inci , Bristow Laurel , Salazar Luis , Al-Husein Zayna , Anderson Evan , Benyeogor Ifeyinwa K , Cheng Andrew , Choi Jong-Ha , De Castro Khalel , Drobeniuc Ana , Hellmeister Kieffer , Kay Ariel , Lee Matthew , Patel Vikash , Reese Olivia D , Smith Veronica , Tanios Ralph , Taylor Elizabeth Grace , Taylor Megan , Washington Wesley , Zhang Cecilia , Johnson Kelly D , Weiss Thomas , Roberts Craig , Ruggieri Madelyn 
Published: July 2024

Abstract

Introduction
Understanding the pneumococcal serotypes causing community-acquired pneumonia (CAP) is essential for evaluating the impact of pneumococcal vaccines.

Methods
We conducted a prospective surveillance study of adults aged ≥18 years hospitalized with CAP at 3 hospitals in Tennessee and Georgia between 1 September 2018 and 31 October 2022. We assessed for pneumococcal etiology with cultures, the BinaxNOW urinary antigen detection test, and serotype-specific urinary antigen detection assays that detect 30 pneumococcal serotypes contained in the investigational pneumococcal conjugate vaccine V116, as well as licensed vaccines PCV15 and PCV20 (except serotype 15B). The distribution of pneumococcal serotypes was calculated based on serotype-specific urinary antigen detection results.

Results
Among 2917 hospitalized adults enrolled with CAP, 352 (12.1%) patients had Streptococcus pneumoniae detected, including 51 (1.7%) patients with invasive pneumococcal pneumonia. The 8 most commonly detected serotypes were: 3, 22F, 19A, 35B, 9N, 19F, 23A, and 11A. Among 2917 adults with CAP, 272 (9.3%) had a serotype detected that is contained in V116, compared to 196 (6.7%) patients with a serotype contained in PCV20 (P &lt; .001), and 168 (5.8%) patients with a serotype contained in PCV15 (P &lt; .001). A serotype contained in V116 but not PCV15 or PCV20 was detected in 120 (4.1%) patients, representing 38.0% of serotype detections.

Conclusions
Approximately 12% of adults hospitalized with CAP had S. pneumoniae detected, and approximately one-third of the detected pneumococcal serotypes were not contained in PCV15 or PCV20. Development of new pneumococcal vaccines with expanded serotype coverage has the potential to prevent a substantial burden of disease.