bluuebunny/crossref_metadata_2025_split huggingface dataset:
TLR4 Signaling Shapes B Cell Dynamics via MyD88-Dependent Pathways and Rac GTPases
Authors: Barrio Laura , Saez de Guinoa Julia , Carrasco Yolanda R. 
Published: August 2013

Abstract
B cells use a plethora of TLR to recognize pathogen-derived ligands. These innate signals have an important function in the B cell adaptive immune response and modify their trafficking and tissue location. The direct role of TLR signaling on B cell dynamics nonetheless remains almost entirely unknown. In this study, we used a state-of-the-art two-dimensional model combined with real-time microscopy to study the effect of TLR4 stimulation on mouse B cell motility in response to chemokines. We show that a minimum stimulation period is necessary for TLR4 modification of B cell behavior. TLR4 stimulation increased B cell polarization, migration, and directionality; these increases were dependent on the MyD88 signaling pathway and did not require ERK or p38 MAPK activity downstream of TLR4. In addition, TLR4 stimulation enhanced Rac GTPase activity and promoted sustained Rac activation in response to chemokines. These results increase our understanding of the regulation of B cell dynamics by innate signals and the underlying molecular mechanisms.
The Swiss Transplant Cohort Study's Framework for Assessing Lifelong Psychosocial Factors in Solid-Organ Transplants
Authors: De Geest Sabina , Burkhalter Hanna , Berben Lut , Bogert Laura Jane , Denhaerynck Kris , Glass Tracy R. , Goetzmann Lutz , Kirsch Monika , Kiss Alexander , Koller Michael T. , Piot-Ziegler Chantal , Schmidt-Trucksäss Arno ,   For the Psychosocial Interest, Group, Swiss Transplantm, Cohort Study
Published: August 2013

Background Understanding outcomes after transplant requires a biopsychosocial model that includes biomedical and psychosocial factors. The latter, to date, are assessed only in a limited way as part of transplant registries or cohort studies. The Swiss Transplant Cohort Study (STCS) is a nationwide open cohort study (starting May 2008) to systematically and prospectively assess psychosocial factors. This article describes the framework underpinning STCS's psychosocial assessment. Methods The STCS framework was adapted from the multidimensional conceptual perspective of Dew et al to describe transplant psychosocial domains and specific outcomes by adding a time perspective, a system perspective, and interaction among domains. Results We propose a multidimensional, multilevel biopsychosocial framework representing mutually influencing domains from before to after transplant, and exemplify each domain by factors included in STCS and their measurement. The transplant patient, centrally positioned, is described by clinical and sociodemographic characteristics (eg, socioeconomic status, educational, professional, and relationship status). The following psychosocial domains further describe the patient: (1) physical/functional (eg, perceived health status, sleep quality, daytime sleepiness), (2) psychological (eg, depression, stress), (3) behavioral (eg, medication adherence, smoking, drug use, physical activity, sun protection), (4) social (eg, work capacity/return to work), and (5) global quality of life. Factors associated with health care system level (eg, trust in transplant team) are also included in the model. Conclusion The STCS's psychosocial framework provides a basis for studying the interplay of biomedical, sociodemographic, psychosocial, behavioral, and health care system factors in view of transplant outcomes and therefore has the potential to guide biopsychosocial transplant research.
Families' Knowledge, Attitudes, and Experiences of the Tissue Donation Process
Authors: Luo Jingjing , Rothnie Alison , Sanderson Elaine , Smith Melissa , Cowie Anne , Musiello Toni 
Published: August 2013

Context Most existing research on the experiences of donor families has been focused on organ donation, with few studies pertaining specifically to the experiences of tissue donor families. Further investigation into tissue donation in Australia is needed in order to improve the process, support, and potential rate of tissue donation consent. Objective To assess the experiences of families of recent tissue donors, to compare changes in the tissue donation process with previously published research, and to identify areas for improvement. Design Cross-sectional survey by postal questionnaire. Setting and Participants Families who consented to tissue donation through DonateLife Western Australia (the coordinating organization for deceased organ and tissue donation in Western Australia) between 2004 and 2009 were invited to participate. Main Outcome Measures Donor families' experience, knowledge, and attitudes toward tissue donation and their satisfaction with the approach and quality/effectiveness of support services provided to donor families. Results One hundred one (39%) of the 256 questionnaires were completed. Most respondents were satisfied with how they were approached, whether by phone or in person. Most participants who received ongoing support after donation (91%) found it beneficial. Nearly half (46%) of respondents supported the use of tissue for research; however, only 28% could recall the topic being discussed at the time of donation. Only 40% of respondents knew of the differences between organ and tissue donation before the donation process. Overall, respondents rated the experience of donating tissue as positive.
Achievements and Barriers in the Organ Donation Process: A Critical Analysis of Donation Coordinators' Discourse
Authors: Mercado-Martínez Francisco J. , Díaz-Medina Blanca A. , Hernández-Ibarra Eduardo 
Published: August 2013

Context Donation coordinators play an important role in the success or failure of organ donation and transplant programs. Nevertheless, these professionals' perspectives and practices have hardly been explored, particularly in low- and middle-income countries. Objective To examine donation coordinators' discourse on the organ donation process and the barriers they perceive. Design A critical qualitative study was carried out in Guadalajara, Mexico. Setting and Participants Twelve donation coordinators from public and private hospitals participated. Data Gathering and Analysis Data were gathered by using semistructured interviews and critical discourse analysis. Results Participants indicated that partial results have been achieved in deceased organ donation. Concomitantly, multiple obstacles have adversely affected the process and outcomes: at the structural level, the fragmentation of the health system and the scarcity of financial and material resources; at the relational level, nonegalitarian relationships between coordinators and hospital personnel; at the ideational level, the transplant domain and its specialists overshadow the donation domain and its coordinators. Negative images are associated with donation coordinators. Conclusions Organ donation faces structural, relational, and ideational barriers; hence, complex interventions should be undertaken. Donation coordinators also should be recognized by the health system.
Attitudes of Australian Emergency Department Clinicians toward Organ and Tissue Donation: An Analysis of Cultural and Religious Influences
Authors: Weiland Tracey J. , Marck Claudia H. , Jelinek George A. , Neate Sandra L. , Hickey Bernadette B. 
Published: August 2013

Objectives To determine Australian emergency department clinicians' cultural and religious barriers to organ and tissue donation (OTD). Method A national cross-sectional survey of physicians and nurses working in Australian emergency departments. An online questionnaire of 133 items was delivered via e-mail. Results Responses were received from 599 of 2969 (20%) physicians and 212 of 1026 (21%) nurses. Respondents were generally representative of the colleges. Participants were from 26 cultures and 9 religious groups. Self-rated competence in OTD tasks was low for some minority groups: South American, Caribbean, and Pacific Islanders reported little competence in identifying, referring, and caring for potential donors, introducing OTD to families, and approaching distressed families. Those of Arabic, Jewish, North African, and Middle Eastern background reported low competence in referring and caring for potential donors and comforting distressed families. They reported low support for OTD after cardiac death, low familiarity with OTD processes after cardiac death, and poor familiarity with the coroner's process. Those of Southern Asian background reported low comfort in undertaking OTD processes, poor familiarity with the coroner's process, and low competence in caring for potential donors. Those of Islamic faith reported low competence in identifying potential donors, low support for OTD after cardiac death, and thought that the emergency department was an inappropriate location to identify potential donors. Those of Buddhist and Hindu faiths reported low competence in identifying potential donors. Respondent numbers for members of minority groups were low, thereby limiting the statistical accuracy of results. Conclusions Among clinicians working in Australian emergency departments, religion and culture may be barriers to facilitating OTD in emergency departments because of the perceptions and attitudes held by particular religious and cultural groups. Improving access to education may address these differences.
Validation of a Single Item to Assess Daytime Sleepiness for the Swiss Transplant Cohort Study
Authors: Burkhalter Hanna , Wirz-Justice Anna , Cajochen Christian , Weaver Terri , Steiger Jürg , Fehr Thomas , Venzin Reto Martin , De Geest Sabina 
Published: August 2013

Context Daytime sleepiness in kidney transplant recipients has emerged as a potential predictor of impaired adherence to the immunosuppressive medication regimen. Thus there is a need to assess daytime sleepiness in clinical practice and transplant registries. Objective To evaluate the validity of a single-item measure of daytime sleepiness integrated in the Swiss Transplant Cohort Study (STCS), using the American Educational Research Association framework. Methods Using a cross-sectional design, we enrolled a convenience sample of 926 home-dwelling kidney transplant recipients (median age, 59.69 years; 25%-75% quartile [Q25-Q75], 50.27–59.69), 63% men; median time since transplant 9.42 years (Q25-Q75, 4.93–15.85). Daytime sleepiness was assessed by using a single item from the STCS and the 8 items of the validated Epworth Sleepiness Scale. Receiver operating characteristic curve analysis was used to determine the cutoff for the STCS daytime sleepiness item against the Epworth Sleepiness Scale score. Results Based on the receiver operating characteristic curve analysis, a score greater than 4 on the STCS daytime sleepiness item is recommended to detect daytime sleepiness. Content validity was high as all expert reviews were unanimous. Concurrent validity was moderate (Spearman ϱ, 0.531; P < .001) and convergent validity with depression and poor sleep quality although low, was significant (ϱ, 0.235; P < .001 and ϱ, 0.318, P= .002, respectively). For the group difference validity: kidney transplant recipients with moderate, severe, and extremely severe depressive symptom scores had 3.4, 4.3, and 5.9 times higher odds of having daytime sleepiness, respectively, as compared with recipients without depressive symptoms. Conclusion The accumulated evidence provided evidence for the validity of the STCS daytime sleepiness item as a simple screening scale for daytime sleepiness.
Lithium Chloride
Authors: Nowick James S. , Lutterbach Guido , Barbay J. Kent , He Wei , Liu Jinlin , Huang Liangbin 
Published: July 2024

Abstract

image

[

7447‐41‐8

]

LiCl
(MW 42.39)

InChI =
1S/ClH.Li/h1H;/q;+1/p‐1

InChIKey =
KWGKDLIKAYFUFQ‐UHFFFAOYSA‐M

(source of Cl
−
as nucleophile and ligand; weak Lewis acid that modifies the reactivity of enolates, lithium dialkylamides, and other Lewis bases)

Physical Data
: mp 605 °C; bp 1325–1360 °C;
d
2.068 g cm
−3
.

Solubility
: very sol H
2
O; sol methanol, ethanol, acetone, acetonitrile, THF, DMF, DMSO, HMPA.

Form Supplied in
: white solid, widely available.

Drying
: deliquescent; for most applications, drying at 150 °C for 3 h is sufficient; for higher purity, recrystallization from methanol, followed by drying at 140 °C/0.5 mmHg overnight, is recommended.

Handling, Storage, and Precautions
: of low toxicity; take directly from the oven when dryness is required.
1‐Chloro‐3,3‐Dimethylbenziodoxole
Authors: Thai‐Savard Léa 
Published: July 2024

image

[69352‐04‐1]

C
9
H
10
ClIO

(MW 296.53)

InChI =
1S/C9H10ClIO/c1‐9(2)7‐5‐3‐4‐6‐8(7)11(10)12‐9/h3‐6H,1‐2H3

InChIKey =
HEBYOYIIQNKPGQ‐UHFFFAOYSA‐N

(key intermediate for hypervalent iodine(III) compounds)

Physical Data
: 178.5–180.0 °C.

Solubility
: dichloromethane, carbon tetrachloride, ethyl acetate, pentane, and most organic solvents.

Form Supplied in
: yellow powder, commercially available.

Analysis of Reagent Purity
:
1
H NMR (300 MHz, CDCl
3
, 23 °C) δ 7.79 (m, 1
H
), 7.99 (ddd,
J
= 8.6, 7.2, and 1.6 Hz, 1
H
), and 8.23 (ddd,
J
= 14.0, 8.0, and 1.3 Hz, 2
H
).
13
C NMR (75.5 MHz, CDCl
3
, 23 °C) δ 117.1, 126.9, 128.8, 131.9, 133.4, 136.6, and 167.2.

Preparative Method
: chlorination of 2‐iodobenzoic acid using trichloroisocyanuric acid (TCICA) (eq 1).

1

image

A 3 L, three‐necked, round‐bottom flask equipped with a magnetic stirring bar, gas inlet, Dimroth condenser, and drop funnel with pressure‐equalizing side arm was charged, under argon with solid 2‐iodobenzoic acid (200 g, 0.790 mol), and anhydrous MeCN (1.5 L) was added. The resulting stirred suspension was heated to 75 °C in an oil bath. The dropping funnel was charged with a solution of trichloroisocyanuric acid (63.7 g, 0.266 mol) in anhydrous MeCN (300 mL). The solution of trichloroisocyanuric acid was dropped into the vigorously stirring reaction mixture within 5 min. The reaction mixture was refluxed for an additional 5 min. The reaction media was vacuum filtered over an oven‐preheated, sintered‐glass funnel with a tightly packed pad of Celite (1 cm thick), and the filter cake was rinsed with additional hot MeCN (100–200 mL). The combined filtrates were evaporated to near‐dryness, and the resulting yellow solid was filtered over a sintered‐glass funnel and washed with a small amount of cold MeCN. The mother liquor from filtration was partially concentrated, giving a second crop of crystals. The combined crops were dried for 2 h under high vacuum to give 1‐chloro‐3,3‐dimethylbenziodoxole (223 g, 89%) as free‐flowing light‐yellow crystals.

Purification
: recrystallization in acetonitrile.

Handling, Storage, and Precautions
: air stable.
Anethole Regulated Crystallization for High Efficiency Carbon‐Based Perovskite Solar Cells
Authors: Hong Jin , Kang Cuiting , Huang Rong , Wu Zhujie , Li Lingcong , Li Xijie , Rao Huashang , Pan Zhenxiao , Zhong Xinhua 
Published: July 2024

AbstractTwo‐step sequential deposition is a widespread technique for the fabrication of perovskite films, renowned for its better control of the crystallization process. However, achieving a well‐controlled and complete reaction of PbI2 by organic ammonium salts remains a key challenge. Previous studies have predominantly focused on regulating the properties of the PbI2 layer while paying less attention to the high reactivity of organic ammonium salts. In this study, the natural molecule anethole is first explored to control perovskite crystallization during two‐step sequential deposition, focusing on the reactivity modulation of organic formamidine ion (FA+). It is demonstrated that FA+ exhibits strong hydrogen bond interactions with anethole, inhibiting the high reactivity of FA+ and effectively delaying the rapid reaction between FAI and PbI2. This decelerates the crystallization kinetics of perovskite films, facilitating the orderly and complete reaction of PbI2 by FAI while suppressing detrimental δ‐phase formation. Consequently, FA‐based perovskite films with high crystallinity, preferred orientation, and low defect state density are obtained. The fabricated planar hole transport layer‐free carbon electrode perovskite solar cells deliver an efficiency of 20.41% (certified efficiency of 20.0%), which is a new record for this kind of solar cell.
Electrically Insulating Electromagnetic Interference Shielding Materials: A Perspective
Authors: Liu Ji , Nicolosi Valeria 
Published: July 2024

AbstractElectromagnetic interference (EMI) shielding is critical in electronic applications. Over the past decades, numerous high‐performance EMI shields are developed using electrically conducting materials. However, from an application aspect, EMI shielding materials, integral to the increasingly complex electronic systems, often need to be electrically insulating to prevent short‐circuiting and enhance overall application reliability and flexibility. This requirement has frequently been overlooked in earlier research on EMI shielding materials. This paper summarizes the representative advancements in the design of innovative EMI shielding materials that minimize and eliminate reliance on electrical conductivity. Insights into the future prospects and directions for the development of electrically insulating EMI shielding materials are also provided, with the aim of pioneering materials that are both intrinsically insulating and effective at EMI shielding.
Co‐Substitution Engineering Boosting the Kinetics and Stablity of VO2 for Zn Ion Batteries
Authors: Wang Zihan , Cui Peng , Wang Xiaomei , Chang Mengwei , Yu Yongren , You Junhua , Hu Fang , Wu Yusheng , Zhu Kai 
Published: July 2024

AbstractVO2 is considered as one of the most likely cathode materials to be commercialized for large‐scale application in AZIBs and is at the forefront of aqueous batteries, but its lower electrical conductivity, slower Zn2+ mobility, as well as voltage degradation and structural collapse due to vanadium solubilization have limited its further development. Herein, a Co‐substitution engineering strategy is proposed, which is introducing heteroatom Co2+ doping substitution and oxygen vacancy substitution to stabilize structure and promote ionic/electronic conductivity, leading an enhanced Zn ion storage behavior. The Co‐substituted VO2 (Co0.03V0.97O2‐x, denote as Ov‐CoVO) is reported in this paper, Co‐substitution inhibits vanadium dissolution of VO2 in AZIBs, even in the acetionitrile system. DFT calculations show that Ov‐CoVO has a more stable structure as well as a faster electronic/ionic conductivity. Consequently, the Ov‐CoVO||ZnOTF||Zn battery (aqueous) can deliver a remarkable capacity of 475 mAh g−1 at 0.2 A g−1 with 99.1% capacity retention after 200 cycles, still maintains excellent cycling stability in Ov‐CoVO||ZnTFSI||Zn (acetionitrile electrolyte) at 0.1 A g−1. In addition, compared to VO2, the charge transfer resistance and Zn2+ iffusion coefficient of Ov‐CoVO are significantly enhanced. This work broadens the scope for research cathode materials for high performance ZIBs.
A Nanoreactor Based on Metal–Organic Frameworks With Triple Synergistic Therapy for Hepatocellular Carcinoma
Authors: Zheng Heming , Huang Lei , An Guanghui , Guo Lianshan , Wang Nannan , Yang Wenhui , Zhu Yanqiu 
Published: July 2024

AbstractThe transformation of monotherapy into multimodal combined targeted therapy to fully exploit synergistic efficacy is of increasing interest in tumor treatment. In this work, a novel nanodrug‐carrying platform based on iron‐based MOFs, which is loaded with doxorubicin hydrochloride (DOX), dihydroartemisinin (DHA), and glucose oxidase (GOx), and concurrently covalently linked to the photosensitizer 5,10,15,20‐tetrakis(4‐carboxyphenyl)porphyrin (TCPP) in polydopamine (PDA)‐encapsulated MIL‐101(Fe) (denoted as MIL‐101(Fe)‐DOX‐DHA@TCPP/GOx@PDA, MDDTG@P), is successfully developed. Upon entering the tumor microenvironment, MDDTG@P catalyzes the hydrogen peroxide (H2O2) into hydroxyl radicals (·OH) and depletes glutathione (GSH); thus, exerting the role of chemodynamic therapy (CDT). The reduced Fe2+ can also activate DHA, further expanding CDT and promoting tumor cell apoptosis. The introduced GOx will rapidly consume glucose and oxygen (O2) in the tumor; while, replenishing H2O2 for Fenton reaction, starving the cancer cells; and thus, realizing starvation and chemodynamic therapy. In addition, the covalent linkage of TCPP endows MDDTG@P with good photodynamic therapeutic (PDT) properties. Therefore, this study develops a nanocarrier platform for triple synergistic chemodynamic/photodynamic/starvation therapy, which has promising applications in the efficient treatment of tumors.
Compliant DNA Origami Nanoactuators as Size‐Selective Nanopores
Authors: Yu Ze , Baptist Anna V. , Reinhardt Susanne C. M. , Bertosin Eva , Dekker Cees , Jungmann Ralf , Heuer‐Jungemann Amelie , Caneva Sabina 
Published: July 2024

AbstractBiological nanopores crucially control the import and export of biomolecules across lipid membranes in cells. They have found widespread use in biophysics and biotechnology, where their typically narrow, fixed diameters enable selective transport of ions and small molecules, as well as DNA and peptides for sequencing applications. Yet, due to their small channel sizes, they preclude the passage of large macromolecules, e.g., therapeutics. Here, the unique combined properties of DNA origami nanotechnology, machine‐inspired design, and synthetic biology are harnessed, to present a structurally reconfigurable DNA origami MechanoPore (MP) that features a lumen that is tuneable in size through molecular triggers. Controllable switching of MPs between 3 stable states is confirmed by 3D‐DNA‐PAINT super‐resolution imaging and through dye‐influx assays, after reconstitution of the large MPs in the membrane of liposomes via an inverted‐emulsion cDICE technique. Confocal imaging of transmembrane transport shows size‐selective behavior with adjustable thresholds. Importantly, the conformational changes are fully reversible, attesting to the robust mechanical switching that overcomes pressure from the surrounding lipid molecules. These MPs advance nanopore technology, offering functional nanostructures that can be tuned on‐demand – thereby impacting fields as diverse as drug delivery, biomolecule sorting, and sensing, as well as bottom‐up synthetic biology.
Eco‐Friendly Production of 2D Ti3C2Tx MXene and Cytotoxicity Mitigation Toward Biomedical Applications
Authors: Sankar Siva Nemala , Araujo Guilherme , Fernandes João , Cerqueira Fatima , Alpuim Pedro , Ribeiro Ana R. , Lebre Filipa , Alfaro‐Moreno Ernesto , Placidi Ernesto , Marras Sergio , Capasso Andrea 
Published: July 2024

Abstract
2D MXenes find applications in several technology fields. Solution processing techniques can facilitate the integration of these materials within the technological supply chain. Here, a highly concentrated (up to 1.5 g L−1) and stable dispersion of 2D Ti3C2Tx MXene flakes is produced by an environmentally friendly liquid phase exfoliation process (LPE) in dihydrolevoglucosenone (Cyrene). The flakes preserve their elemental composition after 6 months, while those exfoliated in N‐Methyl‐2‐pyrrolidone (NMP), a commonly used solvent, undergo significant oxidation. The cytotoxicity of the MXene flakes in Cyrene and in NMP is investigated by assessing the cellular viability in a human keratinocyte (HaCaT) cell line, which is a relevant model for skin applications. The data show that the MXene flakes in Cyrene induce cytotoxic effects for a dose > 1 µg cm−2, as similarly observed for the MXene flakes in NMP. Efforts are made to overcome this by collecting the MXene flakes and redispersing them in a solution of bovine serum albumin (BSA). MXene in BSA does not affect cellular viability and cell morphology, possibly due to the formation of a protective protein corona around the individual MXene flakes. These findings illustrate an environmentally friendly strategy to produce biocompatible 2D MXene for biomedical applications.