bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prehospital identification of trauma patients with early acute coagulopathy and massive bleeding: results of a prospective non-interventional clinical trial evaluating the Trauma Induced Coagulopathy Clinical Score (TICCS)
Authors: Tonglet Martin L , Minon Jean Marc , Seidel Laurence , Poplavsky Jean Louis , Vergnion Michel 
Published: November 2014

Abstract
Introduction
Identifying patients who need damage control resuscitation (DCR) early after trauma is pivotal for adequate management of their critical condition. Several trauma-scoring systems have been developed to identify such patients, but most of them are not simple enough to be used in prehospital settings in the early post-traumatic phase. The Trauma Induced Coagulopathy Clinical Score (TICCS) is an easy-to-measure and strictly clinical trauma score developed to meet this medical need.

Methods
TICCS is a 3-item clinical score (range: 0 to 18) based on the assessment of general severity, blood pressure and extent of body injury and calculated by paramedics on-site for patients with severe trauma. This non-interventional prospective study was designed to assess the ability of TICCS to discern patients who need DCR. These patients were patients with early acute coagulopathy of trauma (EACT), haemorrhagic shock, massive transfusion and surgical or endovascular haemostasis during hospitalization. Diagnosis of EACT was assessed by both thromboelastometry and conventional coagulation tests.

Results
During an 18-month period, 89 severe trauma patients admitted to the general emergency unit at our hospital were enrolled in the study, but 7 were excluded for protocol violations. Of the 82 remaining patients, 8 needed DCR and 74 did not. With receiver operating characteristic curve analysis, TICCS proved to be a powerful discriminant test (area under the curve = 0.98; 95% CI: 0.92 to 1.0). A cutoff of 10 on the TICCS scale provided the best balance between sensitivity (100%; 95% CI: 53.9 to 100) and specificity (95.9%; 95% CI: 88.2 to 99.2). The positive predictive value was 72.7%, and the negative predictive value was 100.0%.

Conclusion
TICCS can be easily and rapidly measured by paramedics at the trauma site. In this study of blunt trauma patients, TICCS was able to discriminate between patients with and without need for DCR. TICCS on-site evaluation should allow initiation of optimal care immediately upon hospital admission of patients with severe trauma in need of DCR. However, a larger multicentre prospective study is needed for in-depth validation of TICCS.

Trial registration
Clinicaltrials.gov ID: NCT02132208 (registered 6 May 2014)
Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples
Authors: Matranga Christian B , Andersen Kristian G , Winnicki Sarah , Busby Michele , Gladden Adrianne D , Tewhey Ryan , Stremlau Matthew , Berlin Aaron , Gire Stephen K , England Eleina , Moses Lina M , Mikkelsen Tarjei S , Odia Ikponmwonsa , Ehiane Philomena E , Folarin Onikepe , Goba Augustine , Kahn S Humarr , Grant Donald S , Honko Anna , Hensley Lisa , Happi Christian , Garry Robert F , Malboeuf Christine M , Birren Bruce W , Gnirke Andreas , Levin Joshua Z , Sabeti Pardis C 
Published: November 2014

AbstractWe have developed a robust RNA sequencing method for generating complete de novo assemblies with intra-host variant calls of Lassa and Ebola virus genomes in clinical and biological samples. Our method uses targeted RNase H-based digestion to remove contaminating poly(rA) carrier and ribosomal RNA. This depletion step improves both the quality of data and quantity of informative reads in unbiased total RNA sequencing libraries. We have also developed a hybrid-selection protocol to further enrich the viral content of sequencing libraries. These protocols have enabled rapid deep sequencing of both Lassa and Ebola virus and are broadly applicable to other viral genomics studies.
Epidemiologic data and pathogen genome sequences: a powerful synergy for public health
Authors: Grad Yonatan H , Lipsitch Marc 
Published: November 2014

AbstractEpidemiologists aim to inform the design of public health interventions with evidence on the evolution, emergence and spread of infectious diseases. Sequencing of pathogen genomes, together with date, location, clinical manifestation and other relevant data about sample origins, can contribute to describing nearly every aspect of transmission dynamics, including local transmission and global spread. The analyses of these data have implications for all levels of clinical and public health practice, from institutional infection control to policies for surveillance, prevention and treatment. This review highlights the range of epidemiological questions that can be addressed from the combination of genome sequence and traditional `line lists’ (tables of epidemiological data where each line includes demographic and clinical features of infected individuals). We identify opportunities for these data to inform interventions that reduce disease incidence and prevalence. By considering current limitations of, and challenges to, interpreting these data, we aim to outline a research agenda to accelerate the genomics-driven transformation in public health microbiology.
YMAP: a pipeline for visualization of copy number variation and loss of heterozygosity in eukaryotic pathogens
Authors: Abbey Darren A , Funt Jason , Lurie-Weinberger Mor N , Thompson Dawn A , Regev Aviv , Myers Chad L , Berman Judith 
Published: November 2014

AbstractThe design of effective antimicrobial therapies for serious eukaryotic pathogens requires a clear understanding of their highly variable genomes. To facilitate analysis of copy number variations, single nucleotide polymorphisms and loss of heterozygosity events in these pathogens, we developed a pipeline for analyzing diverse genome-scale datasets from microarray, deep sequencing, and restriction site associated DNA sequence experiments for clinical and laboratory strains of Candida albicans, the most prevalent human fungal pathogen. The YMAP pipeline (http://lovelace.cs.umn.edu/Ymap/) automatically illustrates genome-wide information in a single intuitive figure and is readily modified for the analysis of other pathogens with small genomes.
Cooperative functions of the two F-BAR proteins Cip4 and Nostrin in regulating E-cadherin in epithelial morphogenesis
Authors: Zobel Thomas , Brinkmann Klaus , Koch Nicole , Schneider Katharina , Seemann Eric , Fleige Astrid , Qualmann Britta , Kessels Michael M. , Bogdan Sven 
Published: November 2014

F-BAR proteins are prime candidates to regulate membrane curvature and dynamics during different developmental processes.
Here, we analyzed nostrin (nost), a novel Drosophila F-BAR protein related to Cip4. Genetic analyses revealed a strong synergism between nost and cip4 functions. While single mutant flies are viable and fertile, combined loss of nost and cip4 results in reduced viability and fertility. Double mutant escaper flies show enhanced wing polarization defects and females exhibit strong egg chamber encapsulation defects. Live-imaging analysis suggests that the observed phenotypes are caused by an impaired E-cadherin membrane turnover. Simultaneous knock-down of Cip4 and Nostrin strongly increases the formation of tubular E-cadherin vesicles at adherens junctions. Cip4 and Nostrin localize at distinct membrane subdomains. Both proteins prefer similar membrane curvatures but seem to form different membrane coats and do not heterooligomerize. Our data suggest an important synergistic function of both F-BAR proteins in membrane dynamics. We propose a cooperative recruitment model in which first Cip4 promotes membrane invagination and early actin-based endosomal motility while Nostrin makes contact with microtubules through the kinesin Khc-73 for trafficking of recycling endosomes.
Influence of Surface Protonation–Deprotonation Stimuli on the Chiroptical Responses of (R)-/(S)-Mercaptosuccinic Acid-protected Gold Nanoclusters
Authors: Ueno Ryota , Yao Hiroshi 
Published: November 2014

Abstract
Racemic mixtures of mercaptosuccinic acid (MSA) are optically resolved to obtain enantiopure (R)-/(S)-MSA, and the (R)-/(S)-MSA-protected gold nanoclusters are synthesized. Circular dichroism responses of the nanoclusters are strongly dependent on the pH of the solution. In particular, chiroptical enhancement is observed under highly acidic conditions, which can be due both to the electronic and conformational origins of the surface chiral MSA ligand.
Visible-light-driven Fuel Cell Constructed by Germanium(IV) Porphyrin/TiO2 Electrode Using Alcohols as an Electron Donor
Authors: Shiragami Tsutomu , Ozawa Hiroki , Kajihara Kurumi , Matsumoto Jin , Yasuda Masahide 
Published: November 2014

Abstract
Visible-light irradiation of a Ge–porphyrin (1a)/TiO2/ITO anode in photochemical cell induced the generation of short-circuit currents in the presence of aliphatic alcohols as an electron donor. The oxidation of alcohols with the Ge–oxyl complex formed by photoinduced electron transfer from 1a to TiO2 could be a critical process for the production of electricity.
X-ray Crystallographic Elucidation for the Alkaline High-spin State Transition of Iron(III) Cytochrome c′ from Alcaligenes xylosoxidans NCIMB 11015
Authors: Takashina Akiko , Unno Masaki , Kohzuma Takamitsu 
Published: November 2014

Abstract
The first X-ray crystallographic study for the alkaline structure transition of cytochrome c′ was performed. Precise X-ray crystal structures of acidic (pH 6.0) and alkaline (pH 10.4) iron(III) cytochrome c′ from Alcaligenes xylosoxidans NCIMB 11015 (AxCyt c′) were determined at 1.1 and 1.48 Å resolution, respectively. The structure of AxCyt c′ at pH 10.4 provides direct evidence for the alkaline high-spin transition caused by bond length elongation between the heme iron and the axial His120 imidazole nitrogen atom.
Copper-catalyzed Silylative Allylation of Ketones and Aldehydes Employing Allenes and Silylboranes
Authors: Tani Yosuke , Yamaguchi Tatsuya , Fujihara Tetsuaki , Terao Jun , Tsuji Yasushi 
Published: November 2014

Abstract
The copper-catalyzed silylative allylation of ketones with allenes and silylboranes via an in situ generated β-silylallylcopper intermediate has been developed. Various ketones and allenes were converted to the homoallylic tertiary alcohols bearing internal (E)-vinylsilane moieties regio- and stereoselectively in good to high yields. Aldehydes were also employed as electrophiles in the reaction.
Council House Movie Star: Que(e)rying the Costume
Authors: Edward Mark 
Published: November 2014

Abstract
Council House Movie Star (2012) originally started as a film enquiry exploring what happens when drag queens age, both off stage and onstage. The research expanded to include two further practice projects: an immersive gallery installation of a life-size council house and a fine art exhibition of the naked and costumed drag body. This article examines the quotidian experiences of a white working-class drag hero/ine and the costumed genderqueered skin. It discusses the queer costume of drag queens, including make-up and wigs. The article also explores the position of memory within the formation of costume for performance as a major theme within the creative processes and design of this project. This visual essay narrates the positioning of drag queens within the social realities of working-class life, thus producing an interesting contrast between the costume of chavs, B-boys and contemporary youth, against the queer and camp drag costume.
Repetition as a methodology: Costumes, archives and choreography
Authors: Fensham Rachel 
Published: November 2014

Abstract
This article considers how costumes contribute to choreographic aesthetics through their capacity to be repeated. I develop different conceptions of repetition – replication (copying); representation (appearance within a frame that represents an image); and reproduction (as construction or manufacture) of costume objects and ideas over time. Being interested in the material process of making and wearing costumes, it also investigates how repetition leads to the possibility of invention. Using Walter Benjamin’s concept of the dialectical image to discuss costumes as objects within a dance archive and within live choreography, it examines an early modern dance form called Natural Movement (NM) as well as seminal postmodern works from the 1970s. It elaborates on the iconic functions of costume in contemporary choreography in relation to Roland Barthes’ writings on the ‘fashion system’, and considers how the costume becomes a sign of its own history. Part of this project to understand repetition requires recognition that the movement quality of texture in a garment, actualizes the experience of affective work taking place in choreography. The experience of repetition in the costume-object therefore leads to a more critical response towards the role of costume in dance and performance.
Relevance of correction for drift and day-to-day variation in cystatin C measurement: a post-hoc analysis of the PREVEND cohort, with independent replication in the ESTHER cohort
Authors: Vart Priya , Bakker Stephan J. L. , Schöttker Ben , de Zeeuw Dick , Rothenbacher Dietrich , Brenner Hermann , Heerspink Hiddo J. Lambers , Saum Kai-Uwe , Reijneveld Sijmen A. , Bültmann Ute , Koenig Wolfgang , Gansevoort Ron T. 
Published: November 2014

AbstractDespite standard laboratory quality control, drift and day-to-day variability in cystatin C measurements can be observed. We investigated whether correction for drift and day-to-day variation in cystatin C measurements improves the association of estimated glomerular filtration rate (eGFR) with chronic kidney disease (CKD) risk factors and prognosis.Plasma samples of the PREVEND study (Dutch cohort study, n=8592) were used to measure cystatin C (Gentian assay) on 243 random days. A correction factor was calculated for each measurement day. GFR was estimated with CKD-EPI equation using routinely measured cystatin C (eGFRCompared to non-reclassified participants, participants re-classified upward had significantly lower age, body mass index, blood pressure, cholesterol, glucose and albuminuria, whereas the opposite was true for participants reclassified downward. CKD risk factors explained more variance in eGFRCorrection for drift and day-to-day variation in cystatin C measurement improves eGFR using cystatin C for its association with CKD risk factors and incident cardiovascular events.
Translation and other acts of meaning: In between cognitive semiotics and semiotics of culture
Authors: Sonesson Göran 
Published: November 2014

AbstractIf translation is an act of meaning transaction, semiotics should be able to define its specificity in relation to other semiotic acts. Instead, following upon suggestions by Roman Jakobson, the Tartu school, and, more implicitly, Charles Sanders Peirce, the notion of translation has been generalized to cover more or less everything that can be done within and between semiotic resources. In this paper, we start out from a definition of communication elaborated by the author in an earlier text, characterizing translation as a double act of meaning. This characterization takes into account the instances of sending and receiving of both acts involved: the first one at the level of cognition and the second one at the level of communication. Given this definition, we show that Jakobson’s “intralinguistic translation” is, in a sense, the opposite of translation and that his “intersemiotic translation” has important differences and well as similarities to real translation. We also suggest that “cultural translation” has very little to do with translation proper except, in some cases, at the end of its operation. Peirce’s idea of exchanging signs for other signs is better understood as a characterization of tradition.
What counts as (an instance of) grammaticalization?
Authors: Joseph Brian D. 
Published: November 2014

AbstractI discuss here various ways in which one might devise a counting heuristic for grammaticalization with an eye to testing the quantificational claims that have been made against specific implementations of such a heuristic. More specifically, I address the question of grammaticalization as a phenomenon of individuals versus a phenomenon of speech communities versus a phenomenon of languages. Similarly, I hope to show, once the individual versus group issue is dealt with, that by adopting Haspelmath’s (2004) definition of grammaticalization as the tightening of internal dependencies, and thus a weakening of boundaries, between elements, we are in a better position to undertake a census since linguists have developed a reasonable idea of the sort of grammatical boundaries that need to be posited (word boundaries, clitic boundaries, morpheme boundaries, phoneme-to-phoneme transitions, etc.). Further, this view generalizes to offer a solution to the problematic notion of gradience in grammaticalization - cf. Kuryłowicz’s famous definition of grammaticalization as taking in movement from “less” to “more” grammatical - since linguists have long posited a hierarchy of boundary strength that can be appealed to.
Sex hormones influence expression and function of peroxisome proliferator-activated receptor γ in adipocytes: pathophysiological aspects
Authors: Sato Hiromi , Ishikawa Momoko , Sugai Hana , Funaki Asami , Kimura Yuki , Sumitomo Mai , Ueno Koichi 
Published: November 2014

Abstract
Adipose tissue plays important roles not only in storing fat but also in maintaining metabolic homeostasis by regulating hundreds of biological signaling events and the secretion of various cytokines. One of the central regulators of adipocyte differentiation is peroxisome proliferator-activated receptor γ (PPARγ), which promotes downstream transcriptional activities, such as adiponectin. Disruption of homeostasis leads to the onset of metabolic diseases such as type 2 diabetes and other triggers for metabolic syndrome. Males and post-menopausal females are more likely to be affected with metabolic diseases than pre-menopausal females, suggesting that sex hormones might be involved in the pathogenesis and development of metabolic diseases. Indeed, 17β-estradiol, testosterone, dihydrotestosterone, and their receptors clearly play a role in adipose regulation: they can alter fat distribution and can modify the expression and activities of PPARγ and its downstream adipocytokines. Furthermore, sex hormones affect inflammatory factors such as nitric oxygen, nitric oxygen synthase, and their surrounding components. Sex hormones are also suggested to be involved with sex differences in the efficacy of the PPARγ agonist thiazolidinediones. Therefore, thorough investigation of how sex hormone-dependent regulation of metabolic homeostasis occurs is necessary in order to develop individualized clinical therapies optimized with regard to each patient’s biological condition and drug sensitivities.
Socio-demographic and dietary factors associated with obesity among female university students in Jordan
Authors: Musaiger Abdulrahman O. , Hammad Shatha S. , Tayyem Reema F. , Qatatsheh Ala A. 
Published: November 2014

Abstract
Objective: This study aimed to explore the socio-demographic and dietary factors that may be associated with obesity among female university students in Jordan.
Methods: A cross-sectional survey was carried out among 406 female students recruited from two universities in Jordan. Data were collected through self-reporting, using a previously validated questionnaire. Weight and height were measured and body mass index was calculated to determine the weight status of the participants.
Results: High educational level of mothers [odds ratio (OR)=1.25] and monthly pocket money of more than 200 Jordanian Dinars (OR=1.67) were found to be risk factors for obesity, whereas a sibling ranking of more than six was a protective factor (OR=0.31). Those who were eating from the university cafeteria had double the risk for obesity (OR=2.41) than those who did not. Regular eating of meals and snacking between meals were found to be protective factors (OR ranged from 0.42 to 0.79). Regular consumption of milk products, fruit, canned fruit juices, bakery products and legumes were found to be protective factors. In contrast, the regular consumption of potato chips (OR=1.35), chicken (OR=1.51), and fish (OR=1.45) were found to be risk factors for obesity. Using a chi-square test, none of the factors studied showed significant association with obesity.
Conclusion: A program to promote healthy eating among university students in Jordan should consider the local socio-demographic and food behavior factors that could be related to obesity to ensure the effectiveness of such a program.
Alginate liquid core capsule formation using the simple extrusion dripping method
Authors: Lee Boon-Beng , Ibrahim Rohaida , Chu Sue-Yin , Zulkifli Nurul Ainina , Ravindra Pogaku 
Published: November 2014

Abstract
Liquid core capsules have been widely used in various biotechnological applications. The capsules could be formed by the simple extrusion dripping method. However, the method requires strict control of several process variables in order to form spherical capsules. The aim of this study was to systematically investigate the influence of process variables of the method on the capsule size and shape. The results showed that the capsules diameter was decreased when the concentration of alginate solution was increased. The capsule diameter was increased when the gelation time and dripping tip diameter were increased. The membrane thickness of the capsules was significantly increased by the concentration of calcium chloride, gelation time and dripping tip diameter. However, the concentration of alginate gave the opposite trend on the membrane thickness of the capsules. As a recommendation, uniform and spherical alginate liquid core capsules could be formed when concentration of calcium chloride was >10 g/l, the concentration of alginate solution was >5 g/l and <20 g/l, gelation solution height in between 1.7 cm and 3.2 cm, and stirring rate of the gelation bath was in the range of 400–500 rpm.
Effect of Fuel Particle Size on the Stability of Swirl Stabilized Flame in a Gas Turbine Combustor
Authors: Mishra R. K. , Kishore Kumar S. , Chandel Sunil 
Published: November 2014

AbstractCombustion stability is examined in a swirl stabilized aero gas turbine combustor using computational fluid dynamics. A 22.5° sector of an annular combustor is modeled for the study. Unstructured tetrahedral meshes comprising 1.2 × 10