bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptome and Complexity-Reduced, DNA-Based Identification of Intraspecies Single-Nucleotide Polymorphisms in the Polyploid Gossypium hirsutum L.
Authors: Zhu Qian-Hao , Spriggs Andrew , Taylor Jennifer M , Llewellyn Danny , Wilson Iain 
Published: August 2014

Abstract
Varietal single nucleotide polymorphisms (SNPs) are the differences within one of the two subgenomes between different tetraploid cotton varieties and have not been practically used in cotton genetics and breeding because they are difficult to identify due to low genetic diversity and very high sequence identity between homeologous genes in cotton. We have used transcriptome and restriction site−associated DNA sequencing to identify varietal SNPs among 18 G. hirsutum varieties based on the rationale that varietal SNPs can be more confidently called when flanked by subgenome-specific SNPs. Using transcriptome data, we successfully identified 37,413 varietal SNPs and, of these, 22,121 did not have an additional varietal SNP within their 20-bp flanking regions so can be used in most SNP genotyping assays. From restriction site−associated DNA sequencing data, we identified an additional 3090 varietal SNPs between two of the varieties. Of the 1583 successful SNP assays achieved using different genotyping platforms, 1363 were verified. Many of the SNPs behaved as dominant markers because of coamplification from homeologous loci, but the number of SNPs acting as codominant markers increased when one or more subgenome-specific SNP(s) were incorporated in their assay primers, giving them greater utility for breeding applications. A G. hirsutum genetic map with 1244 SNP markers was constructed covering 5557.42 centiMorgan and used to map qualitative and quantitative traits. This collection of G. hirsutum varietal SNPs complements existing intra-specific SNPs and provides the cotton community with a valuable marker resource applicable to genetic analyses and breeding programs.
Genetic Regulation ofZfp30, CXCL1, and Neutrophilic Inflammation in Murine Lung
Authors: Rutledge Holly , Aylor David L , Carpenter Danielle E , Peck Bailey C , Chines Peter , Ostrowski Lawrence E , Chesler Elissa J , Churchill Gary A , de Villena Fernando Pardo-Manuel , Kelada Samir N P 
Published: August 2014

AbstractAllergic asthma is a complex disease characterized in part by granulocytic inflammation of the airways. In addition to eosinophils, neutrophils (PMN) are also present, particularly in cases of severe asthma. We sought to identify the genetic determinants of neutrophilic inflammation in a mouse model of house dust mite (HDM)-induced asthma. We applied an HDM model of allergic asthma to the eight founder strains of the Collaborative Cross (CC) and 151 incipient lines of the CC (preCC). Lung lavage fluid was analyzed for PMN count and the concentration of CXCL1, a hallmark PMN chemokine. PMN and CXCL1 were strongly correlated in preCC mice. We used quantitative trait locus (QTL) mapping to identify three variants affecting PMN, one of which colocalized with a QTL for CXCL1 on chromosome (Chr) 7. We used lung eQTL data to implicate a variant in the gene Zfp30 in the CXCL1/PMN response. This genetic variant regulates both CXCL1 and PMN by altering Zfp30 expression, and we model the relationships between the QTL and these three endophenotypes. We show that Zfp30 is expressed in airway epithelia in the normal mouse lung and that altering Zfp30 expression in vitro affects CXCL1 responses to an immune stimulus. Our results provide strong evidence that Zfp30 is a novel regulator of neutrophilic airway inflammation.
Neuroinvasive Arboviral Disease in the United States: 2003 to 2012
Authors: Gaensbauer James T. , Lindsey Nicole P. , Messacar Kevin , Staples J. Erin , Fischer Marc 
Published: August 2014

OBJECTIVE:To describe the epidemiologic and clinical syndromes associated with pediatric neuroinvasive arboviral infections among children in the United States from 2003 through 2012.METHODS:We reviewed data reported by state health departments to ArboNET, the national arboviral surveillance system, for 2003 through 2012. Children (&lt;18 years) with neuroinvasive arboviral infections (eg, meningitis, encephalitis, or acute flaccid paralysis) were included. Demographic, clinical syndrome, outcome, geographic, and temporal data were analyzed for all cases.RESULTS:During the study period, 1217 cases and 22 deaths due to pediatric neuroinvasive arboviral infection were reported from the 48 contiguous states. La Crosse virus (665 cases; 55%) and West Nile virus (505 cases; 41%) were the most common etiologies identified. Although less common, Eastern equine encephalitis virus (30 cases; 2%) resulted in 10 pediatric deaths. La Crosse virus primarily affected younger children, whereas West Nile virus was more common in older children and adolescents. West Nile virus disease cases occurred throughout the country, whereas La Crosse and the other arboviruses were more focally distributed.CONCLUSIONS:Neuroinvasive arboviral infections were an important cause of pediatric disease from 2003 through 2012. Differences in the epidemiology and clinical disease result from complex interactions among virus, vector, host, and the environment. Decreasing the morbidity and mortality from these agents depends on vector control, personal protection to reduce mosquito and tick bites, and blood donor screening. Effective surveillance is critical to inform clinicians and public health officials about the epidemiologic features of these diseases and to direct prevention efforts.
Impact Locations and Concussion Outcomes in High School Football Player-to-Player Collisions
Authors: Kerr Zachary Y. , Collins Christy L. , Mihalik Jason P. , Marshall Stephen W. , Guskiewicz Kevin M. , Comstock R. Dawn 
Published: August 2014

BACKGROUND:
Little research has examined concussion outcomes in terms of impact location (ie, the area on the head in which the impact occurred). This study describes the epidemiology of concussions resulting from player-to-player collision in high school football by impact location.

METHODS:
National High School Sports-Related Injury Surveillance Study data (2008/2009–2012/2013) were analyzed to calculate rates and describe circumstances of football concussion (eg, symptomology, symptom resolution time, return to play) resulting from player-to-player collisions by impact location (ie, front-, back-, side-, and top-of-the-head).

RESULTS:
Most concussions resulting from player-to-player collisions occurred from front-of-the-head (44.7%) and side-of-the-head (22.3%) impacts. Number of symptoms reported, prevalence of reported symptoms, symptom resolution time, and length of time to return to play were not associated with impact location. However, a larger proportion of football players sustaining concussions from top-of-the-head impacts experienced loss of consciousness (8.0%) than those sustaining concussions from impacts to other areas of the head (3.5%) (injury proportion ratio 2.3; 95% confidence interval 1.2–4.2; P = .008). Players had their head down at the time of impact in a higher proportion of concussions caused by top-of-the-head impacts (86.4%) than concussions from impacts to other areas of the head (24.0%) (injury proportion ratio 3.6; 95% confidence interval 3.2–4.0; P &lt; .001).

CONCLUSIONS:
Among high school football players who sustained concussions due to player-to-player collisions, concussion outcomes were generally independent of impact location. Recommended strategies for reducing the proportion of top-of-the-head impacts include improved education regarding tackling with proper “head-up” technique.
Defining and Determining Medical Necessity in Medicaid Managed Care
Authors: Markus Anne Rossier , West Kristina D. 
Published: August 2014

OBJECTIVES:
In 2013, the American Academy of Pediatrics published a policy statement calling for pediatricians to be informed about the need for specific pediatric medical necessity language because children deserve “the intent embedded in Medicaid.” This study aims to explore the definitions and determinations of medical necessity in Medicaid Managed Care (MMC), document the relevant language used throughout Medicaid, and investigate whether the federal standard of medical necessity for children is replicated in related state documents.

METHODS:
We conducted a desk review of state statutes, model MMC contracts, and 2 provider manuals per state, for 33 states with a full-risk MMC model.

RESULTS:
The federal “to correct and ameliorate” standard was replicated in 100% of state regulations, 72% of MMC model contracts (n = 13 of 18 MMC model contracts available online), and 54% of provider manuals (n = 30 of 56 available and sampled online). Only 9 states had an explicit “preventive” pediatric medical necessity standard in their state regulations that exemplified “the intent imbedded in Medicaid.”

CONCLUSIONS:
The federal medical necessity standard for children is not replicated consistently within MMC programs from the state, to health plans, to network providers. Although the majority of the documents reviewed included the standard, the presence of the standard decreased by almost half between state-level and network-provider-level regulations. Having a single, explicitly defined pediatric medical necessity definition replicated at all levels of the health system would reduce confusion and increase the ability of pediatricians to apply the standard more uniformly.
The Influence of Chemical Surface Treatment on the Corrosion Resistance of Titanium Castings Used in Dental Prosthetics
Authors: Burnat B. , Parchańska-Kowalik M. , Klimek L. 
Published: August 2014

Abstract
Air abrasion process is used for cleaning casting surface of prosthetic components, and to prepare the surface of these elements for the application of veneering items. Its side effect, however, is that abrasive particles are embedded in the treated surface, which can be up to 30% of the surface and it constitutes the side effect of this procedure. Such a significant participation of foreign material can not be indifferent to the properties of the surface. Embedded particles can be the place of stress concentration causing cracking of ceramics, and may deteriorate corrosion resistance by forming corrosive microlinks. In the latter cases, it would be advisable to remove elements embedded into the surface. The simplest method is chemical etching or electrochemical one. Nevertheless, these procedures should not significantly change the parameters of the surface. Among many possible reagents only a few fulfills all the above conditions. In addition, processing should not impair corrosion resistance of titanium, which is one of the most important factors determining its use as a prosthetic restoration in the mouth. The study presented results of corrosion resistance of titanium used to make prosthetic components by means of casting method, which were subjected to chemical processing designed to remove the embedded abrasive particles. The aim of the study was to investigate whether etching with selected reagents affects the corrosion resistance of titanium castings. For etching the following reagents were used: 30% HNO3 + 3% HF + H2O, HNO3+ HF+ glycerol (1:2:3), 4% HF in H2O2, 4% HF in H2O, with a control sandblasted sample, not subjected to etching. Tests demonstrated that the etching affected corrosion properties of test samples, in each case the reduction of the corrosion potential occurred - possibly due to the removal of particles of Al2O3 from the surface and activation of the surface. None of the samples underwent pitting corrosion as a result of polarization to 9 V. Values of the polarization resistance, and potentiodynamic characteristics indicated that the best corrosion resistance exhibited the samples after etching in a mixture of 4% solution of HF in H2O2. They showed very good passivation of the surface.
The Use of Innovative Ceramic-Carbon Bonded Filters Used for Filtration of Liquid Alloys and Evaluation of the Filtration Efficiency
Authors: Karwiński A. , Małysza M. , Wieliczko P. , Gil A. , Lipowska B. 
Published: August 2014

Abstract
Extremely intense development of civilization requires from foundry casting technologies very high quality and not expensive castings. In the foundries, there are many treatments that allow increasing of the final properties of produced castings such as refining, modification, heat treatment, etc. One of the methods of increasing the quality of the casting by removing inclusions from the liquid alloy is filtration. The use of ceramic-carbon foam filters in filtration process is still analysed phenomenon that allows improving the final properties of castings. A modern method of research, testing and synthesis of innovative chemical compositions allows improving the properties of such filters. In the paper the evaluation of application properties of developed ceramic-carbon bonded foam filters is presented. The quality of the foam filters is evaluated by Computer Tomography and foundry trials in pouring of liquid metal in test molds. Additionally computer simulations were made to visualize the flow characteristics in the foam filter. The analysed filters are the result of the research work of Foundry Research Institute and the Institute of Ceramics and Building Materials, Refractory Materials Department in Gliwice.
Multi-Criterion Assessment of Different Variants of Casts Manufacturing Processes
Authors: Kukla S. 
Published: August 2014

Abstract
The paper presents the issue of production processes improvement in foundries in the area of finishing treatment of iron casts manufactured on automated foundry lines with vertical or horizontal mould division. Due to numerous factors which influence the efficiency of the processes, multi-criterion assessment tools were proposed in order to select the optimal solution for the assumed criteria. After determining the criteria weight using the Saaty method, a simulation experiment was designed and carried out which presents possible scenarios of casts finishing treatment operations. Basing on experiment reports from a computer model, particular solutions were evaluated using the Yager's method. The evaluation of the experiment results was performed by experts who assessed different options according to each of the criteria adopted. After the establishment of the total standardized ratings by averaging the scores given by individual experts, the final decision was generated. Using the presented method, the best solution was chosen from among the analyzed scenarios.
The Influence of Graphite Addition on the Abrasive Wear of AlMg10 Alloy Matrix Composites Reinforced with SiC Particles
Authors: Łągiewka M. , Konopka Z. 
Published: August 2014

Abstract
The presented work deals with the influence of the addition of soft graphite particles on the abrasive wear of composite reinforced with hard SiC particles. The discussed hybrid composites were produced by stirring the liquid alloy and simultaneous adding the mixture of particles. The adequately prepared suspension was gravity cast into a metal die. Both the composite castings obtained in this way and the comparative castings produced of the pure matrix alloy were examined for the abrasive wear behaviour. Photomacrographs of the sliding surfaces of the examined composites were taken, and also the hardness measurements were carried out. It was found that even a small addition of Cgr particles influences positively the tribological properties of the examined composite materials, protecting the abraded surface from the destructive action of silicon carbide particles. The work presents also the results of hardness measurements which confirm that the composite material hardness increases with an increase in the volume fraction of hard reinforcing particles.
Evaluation of Heat Capacity and Resistance to Cyclic Oxidation of Nickel Superalloys
Authors: Przeliorz R. , Binczyk F. , Gradoń P. , Góral M. , Mikuszewski T. 
Published: August 2014

Abstract
Paper presents the results of evaluation of heat resistance and specific heat capacity of MAR-M-200, MAR-M-247 and Rene 80 nickel superalloys. Heat resistance was evaluated using cyclic method. Every cycle included heating in 1100°C for 23 hours and cooling for 1 hour in air. Microstructure of the scale was observed using electron microscope. Specific heat capacity was measured using DSC calorimeter. It was found that under conditions of cyclically changing temperature alloy MAR-M-247 exhibits highest heat resistance. Formed oxide scale is heterophasic mixture of alloying elements, under which an internal oxidation zone was present. MAR-M-200 alloy has higher specific heat capacity compared to MAR-M-247. For tested alloys in the temperature range from 550°C to 800°C precipitation processes (γ′, γ″) are probably occurring, resulting in a sudden increase in the observed heat capacity.
High-Strength Aluminium Alloys and Their Use in Foundry Industry of Nickel Superalloys
Authors: Pysz S. , Maj M. , Czekaj E. 
Published: August 2014

Abstract
Of great importance in the selection of materials for cast structures is keeping a proper balance between the mechanical and plastic properties, while preserving the relevant casting properties. This study has been devoted to an analysis of the choice and application of high-strength aluminium-based alloys maintaining sufficient level of casting properties. The high level of tensile strength (Rm > 500 MPa) matched with satisfactory elongation (A > 3%) is important because materials of this type are used for cast parts operating in the aerospace, automotive, and military industries. These beneficial relationships between the high tensile strength and toughness are relatively easy to obtain in the Al-Zn-Mg-Cu alloys subjected to plastic forming and proper heat treatment. In gravity cast products, on the other hand, whether poured into sand moulds or metal moulds (dies), obtaining this favourable combination of properties poses a number of research problems (mostly resulting from the alloy chemical composition) as well as technical and technological difficulties.
The Decision Support System in the Domain of Casting Defects Diagnosis
Authors: Wilk-Kołodziejczyk D. , Rojek G. , Regulski K. 
Published: August 2014

Abstract
This article presents a computer system for the identification of casting defects using the methodology of Case-Based Reasoning. The system is a decision support tool in the diagnosis of defects in castings and is designed for small and medium-sized plants, where it is not possible to take advantage of multi-criteria data. Without access to complete process data, the diagnosis of casting defects requires the use of methods which process the information based on the experience and observations of a technologist responsible for the inspection of ready castings. The problem, known and studied for a long time, was decided to be solved with a computer system using a CBR (Case-Based Reasoning) methodology. The CBR methodology not only allows using expert knowledge accumulated in the implementation phase, but also provides the system with an opportunity to “learn” by collecting new cases solved earlier by this system. The authors present a solution to the system of inference based on the accumulated cases, in which the main principle of operation is searching for similarities between the cases observed and cases stored in the knowledge base.
Investigation of an Advanced Cellulose Profile Used for the Manufacture of Gating Systems
Authors: Zawieja Z. , Sawicki J. , Gumienny G. , Sobczyk-Guzenda A. 
Published: August 2014

Abstract
The herein paper contains the results of investigations on a new type of cellulose blend used for the manufacture of profiles applied in the process of making gating systems in the foundry industry. A standard cellulose profile was subjected to an experiment. During the experiment the profile was filled with a liquid cast iron and at the same time the temperatures of the liquid metal crystallizing inside the profile were measured as well as the temperature of the outer layer of the profile was controlled. Further, the microstructure of the cast iron, which crystallized out inside the cellulose profile, was analysed and the cellulose, thermally degraded after the experiment, was verified with the use of the chemical analysis method. Moreover, a quality analysis of the original as well as the degraded cellulose profile was run with the use of the FTIR infrared spectroscopy. The presented results revealed that the cellulose blend is aluminium silicate enriched and contains organic binder additives. The cast iron, which crystallized out, tended to have an equilibrium pearlitic structure with the release of graphite and carbides. The generation of disequilibrium ausferrite phases was also observed in the structure.
Distribution and Morphological Variations of Invasive Macrophytes Elodea Nuttallii (Planch.) H. St. John and Elodea Canadensis Michx in Croatia
Authors: Kočić Aleksandra , Horvatić Janja , Jelaska Sven D. 
Published: August 2014

Abstract
The invasive species Elodea nuttallii was recorded for the first time in the Croatian flora in 2006, in the drainage channels of Kopački rit (Baranja). After its establishment, E. nuttallii begins to spread to the eastern and northern part of the drainage channel network from 2006-2009. High water levels are responsible for the linear spreading direction of E. nuttallii, E. nuttallii and E. canadensis show a wide range of morphological variation, which is characteristic of successful invaders. To show morphological variations of two Elodea species, the most important characters indicated in the literature were measured on 24 fresh collected samples from the seven sites in Croatia. In spite of some overlap in leaf length and width between the two Elodea species, the differences of all morphological traits except internode length are statistically significant. In E. nuttallii leaf width, length and internode length show a higher morphological variability as a result of the higher adaptive strategy to environmental parameters. The most reliable morphologi-cal characters distinguishing E. nuttallii and E. canadensis are leaf width 0.5 mm below the tip and the angle at the apex. E. nuttallii can be expected to spread to other areas of Croatia.
Responses of Wheat (Triticum Aestivum L.) and Maize (Zea Mays L.) Plants to Cadmium Toxicity in Relation to Magnesium Nutrition
Authors: Nikolić Nataša , Pajević Slobodanka , Župunski Milan , Topić Mirjana , Arsenov Danijela 
Published: August 2014

AbstractThe influence of cadmium (Cd) on physiological processes in wheat (Triticum aestivumL.) and maize (Zea maysL.) plants exposed to either optimal mineral nutrition or the absence of magnesium (Mg) as well as the accumulation of cadmium and magnesium in plant organs were studied using the method of water culture in a greenhouse. Cd treatment reduced shoot fresh mass more strongly in Mg-supplied than in Mg-deficient plants. Negative effect of Cd on photosynthetic activity was more pronounced inT. aestivumthan inZ. maysplants. Cd treatment decreased leaf chlorophyll and carotenoid concentration in bothZ. maysandT. aestivum,irrespective of the Mg supply. Cd was preferentially accumulated in the roots of both species. Catalase activity inT. aestivumleaves and roots was unaffected by Cd and Mg supply. Cd treatment did not affect Fe accumulation in the leaves of either species, while in the roots a considerable increase occurred, irrespective of the Mg nutrition. Higher tolerance ofZ. maysandT. aestivumplants to Cd toxicity exposed to Mg deficiency could partly be ascribed to the preservation of Fe nutrition.