bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acclimation to high daily thermal amplitude converts a defense response regulator into susceptibility factor
Authors: Didelon Marie , Sucher Justine , Carvalho-Silva Pedro , Zaffuto Matilda , Barbacci Adelin , Raffaele Sylvain 
Published: August 2024

ABSTRACTAcclimation enables plants to adapt to immediate environmental fluctuations, supporting biodiversity and ecosystem services. However, global changes are altering conditions for plant disease outbreaks, increasing the risk of infections by pathogenic fungi and oomycetes, and often undermining plant immune responses. Understanding the molecular basis of plant acclimation is crucial for predicting climate change impacts on ecosystems and improving crop resilience. Here, we investigated howArabidopsis thalianaquantitative immune responses acclimates to daily temperature fluctuations. We analyzed responses to the fungal pathogenSclerotinia sclerotiorumfollowing three acclimation regimes that reflect the distribution areas of both species. Mediterranean acclimation, characterized by broad diurnal temperature amplitudes, resulted in a loss of disease resistance in three naturalA. thalianaaccessions. Global gene expression analyses revealed that acclimation altered nearly half of the pathogen-responsive genes, many of which were down-regulated by inoculation and associated with disease susceptibility. Phenotypic analysis ofA. thalianamutants identified novel components of quantitative disease resistance following temperate acclimation. Several of these mutants were however more resistant than wild type following Mediterranean acclimation. Notably, mutant lines in the NAC42-like transcription factor did not show a loss of resistance under Mediterranean acclimation. This resistance was linked to an acclimation-mediated switch in the repertoire of NAC42-like targets differentially regulated by inoculation. These findings reveal the rewiring of immune gene regulatory networks by acclimation and suggest new strategies to maintain plant immune function in a warming climate.
Two residues reprogram immunity receptor kinases to signal in nitrogen-fixing symbiosis
Authors: Tsitsikli Magdalini , Simonsen Bine W. , Larsen Maria M. , Andersen Camilla G. , Gysel Kira , Lironi Damiano , Krönauer Christina , Rübsam Henriette , Luu Thi-Bich , Hansen Simon B. , Kaya Gülendam , Stougaard Jens , Andersen Kasper R. , Radutoiu Simona 
Published: August 2024

AbstractReceptor signaling shapes cellular functions and specificity in the signaling output is central for delineating biological processes. In legume root cells, very similar and structurally conserved chitin and Nod factor receptor kinases activate immunity, or symbiosis, respectively after perception of chitinous ligands. Here we show that individual residues in the intracellular part of the Nod factor receptor NFR1 determine signal specificity and efficiency distinguishing immunity and symbiotic signaling. Functional investigation of CERK6, NFR1 and receptor variants hereof revealed a conserved motif that we term Symbiosis Determinant 1 in the juxtamembrane region of the kinase domain that is key for symbiotic signaling. We demonstrate that two residues in Symbiosis Determinant 1 are indispensable hallmarks for NFR1 receptors and are sufficient to convertLotusCERK6 and barley RLK4 functioning in immunity, to initiate symbiotic signaling.
Open-hardware solutions for light sheet microscopy specimen chambers
Authors: Golden Artemiy , Colombelli Julien , Stelzer Ernst H.K. , Pampaloni Francesco 
Published: August 2024

AbstractLight sheet fluorescence microscopy (LSFM) is a powerful tool for imaging large three-dimensional biological samples. However, the design and fabrication of specimen chambers for these systems present significant challenges, particularly in maintaining water-tight seals, preventing contamination, and ensuring the flexibility needed for precise positioning of the objective and sample. This study introduces open-hardware solutions to address these challenges, utilising a combination of 3D printing, silicone injection moulding, and FEP-foil thermoforming. We describe the development of custom, highly flexible silicone seals and connectors through a laboratory-scale injection moulding process. These components enable precise, low-resistance movement of imaging objectives and specimen holders, which is crucial for maintaining imaging accuracy. Additionally, we introduce a novel “optical window” design that isolates the objective lens from the immersion medium, significantly reducing the risk of contamination and facilitating easy exchange of chambers and lenses without compromising sterility. The practicality of these designs is demonstrated through their application in long-term live imaging ofTribolium castaneumembryos, honey bee embryos, and human mesenchymal stem cell spheroids. By providing open-source CAD and 3D printing files, this work promotes accessibility and customization in microscopy, enabling researchers to easily replicate and adapt these solutions to their specific needs.
Biophysical limits of ultrafast cellular motility
Authors: Chang Ray , Prakash Manu 
Published: August 2024

AbstractMany single-celled organisms and specialized cell types can surprisingly achieve speed and acceleration significantly faster than multicellular counterparts. These remarkable cellular machines must integrate energy storage and amplification in actuation, latches for triggered release, and energy dissipation without failure — all implemented in macro-molecular assemblies inside a single cell. However, a universal biophysical framework that can comparatively evaluate extreme cellular motility remains lacking. Scaling laws have long been recognized as powerful tools for revealing universal principles in physical systems. We map the atlas of ultrafast motility for single cells across the tree of life. We then introduce a new quantitative framework that can be used to evaluate and compare extreme acceleration, speed, area strain rate, volume expansion strain rate, and density changes in single cells. Recognizing that many single cells operate in low-Reynolds number environments, we introduce a new dimensionless number, the “cellular acceleration number,” based on energy dissipation at this scale. Using this new framework, we discover a scaling law between the cellular acceleration number and the transient Reynolds number, valid across six orders of magnitude in a range of single-cell organisms. We further generalize these ideas by placing various trigger, actuation, and dissipation mechanisms within the same framework and estimating the fundamental limits of speed and acceleration at the cellular scale. We conclude with a detailed summary of the range of functions implemented via ultrafast cellular phenomena, laying down a quantitative foundation for extreme biophysics at the cellular scale.
Leveraging Chlorination-Based Mechanism for Resolving Subcellular Hypochlorous Acid
Authors: Tang Fung Kit , Tucker Lawrence , Nadiveedhi Maheshwara Reddy , Hladun Colby , Morse Jared , Ali Mahnoor , Payne Noah , Schmidt Matthias , Leung Kaho 
Published: August 2024

AbstractHypochlorous acid (HOCl) is crucial for pathogen defense, but an imbalance in HOCl levels can lead to tissue damage and inflammation. Existing HOCl indicators employ an oxidation approach, which may not truly reveal the chlorinative stress environment. We designed a suite of indicators with a new chlorination-based mechanism, termed HOClSense dyes, to resolve HOCl in sub-cellular compartments. HOClSense dyes allow the visualization of HOCl with both switch-on and switch-off detection modes with diverse emission colors, as well as a unique redshift in emission. HOClSense features a minimalistic design with impressive sensing performance in terms of HOCl selectivity, and our design also facilitates functionalization through click chemistry for resolving subcellular HOCl. As a proof of concept, we targeted plasma membrane and lysosomes with HOClSense for subcellular HOCl mapping. With utilizing HOClSense, we discovered the STING pathway-induced HOCl production and the abnormal HOCl production in Niemann-Pick diseases. To the best of our knowledge, this is the first chlorination-based HOCl indicator series for resolving subcellular HOCl.
The applicability of Regional Red List assessments for soil invertebrates: First assessment of five native earthworm species in Canada
Authors: Phillips Helen R. P. , Brown George G. , James Sam W. , Mathieu Jérôme , Reynolds John Warren , Dharmasiri Maheshi E. , Singer Claire L. , Briones Maria J. I. , Proctor Heather C. , Cameron Erin K. 
Published: August 2024

AbstractEarthworms (Annelida: Clitellata: Crassiclitellata) are prominent members of the soil community, important to many ecosystem functions. Despite this, and like many other soil invertebrates, they are rarely considered in conservation assessments, including the IUCN Red List assessments used to assess species’ extinction risk. To investigate the applicability of the IUCN Regional Red Listing protocol to soil invertebrates, we assessed the conservation status of five earthworm species known to be native to Canada using this protocol and all available occurrence records. In Canada, no earthworm species have yet been assessed by the Committee on the Status of Endangered Wildlife in Canada (COSEWIC). Due to the lack of data on population sizes and their trends, all five species were assessed using their Extent of Occurrence (EOO) (Criterion B). One species was assessed as Vulnerable, two were assessed in non-threatened categories, and two were assessed as Data Deficient. For the majority, the main threats identified were the continuing loss of potential habitat due to land conversion and resource exploitation, as well as the effects of climate change. Increasing the amount of data, including but not limited to distribution and habitat preferences, would make the assessment process easier and status decisions better supported. By undertaking regional assessments for five native earthworm species in Canada, we show that Regional Red List assessments are feasible for soil invertebrates.
DNA methylation protects cancer cells against senescence
Authors: Chen Xiaoying , Yamaguchi Kosuke , Rodgers Brianna , Goehrig Delphine , Vindrieux David , Lahaye Xavier , Nolot Matthieu , Ferry Laure , Martin Nadine , Dubus Pierre , Miura Fumihito , Ito Takashi , Manel Nicolas , Kanemaki Masato , Bernard David , Defossez Pierre-Antoine 
Published: August 2024

AbstractInhibitors of DNA methylation such as 5-aza-deoxycytidine are widely used in experimental and clinical settings. However, their mechanism of action is such that DNA damage inevitably co-occurs with loss of DNA methylation, making it challenging to discern their respective effects. Here we deconvolute the effects of decreased DNA methylation and DNA damage on cancer cells, by using degron alleles of key DNA methylation regulators. We report that cancer cells with decreased DNA methylation —but no DNA damage— enter cellular senescence, with G1 arrest, SASP expression, and SA-β-gal positivity. This senescence is independent of p53 and Rb, but involves p21, which is cytoplasmic and inhibits apoptosis, and cGAS, playing a STING-independent role in the nucleus. Xenograft experiments show that tumor cells can be made senescentin vivoby decreasing DNA methylation. These findings reveal the intrinsic effects of loss of DNA methylation in cancer cells and have practical implications for future therapeutic approaches.
FGF9 treatment reduces off-target chondrocytes from iPSC-derived kidney organoids
Authors: Joris Virginie , Schumacher Anika , Marks Paula , Eischen-Loges Maria , van Griensven Martijn , LaPointe Vanessa L.S. 
Published: August 2024

SummaryRenal failure due to drug nephrotoxicity or disease is frequently observed in patients. The development ofin vitromodels able to recapitulate kidney biology offers new possibilities to study drug toxicity or model diseases. Induced pluripotent stem cell–derived kidney organoids already show promise, but several drawbacks must be overcome to maintain them in culture, among which is the presence of non-renal cell populations such as cartilage. We modified the culture protocol and maintained kidney organoids in medium containing FGF9 for one additional week compared to the control protocol (Takasato). In comparison to the control, the FGF9-treated kidney organoids had reduced cartilage at day 7+25 and diminished chondrocyte marker expression. Importantly, the renal structures assessed by immunofluorescence were unaffected by the FGF9 treatment. This reduction of cartilage produces a higher quality kidney organoid that can be maintained longer in culture to improve their maturation for furtherin vivowork.HighlightsKidney organoids develop cartilage between days 7+18 and 7+25Extending the FGF9 supplementation reduces cartilage and chondrocyte markersFGF9-treated organoids present less EMT marker expression than control organoidsRenal structures are not affected by the extended FGF9 treatmenteTOC blurbLaPointe and colleagues showed that a one-week extension of FGF9 supplementation in iPSC-derived kidney organoids leads to a reduction of off-target cartilage. The renal structures are not impacted by FGF9 treatment and EMT markers are reduced. This modified organoid protocol will enable longer culture periods, a benefit for the use of organoids for screening or therapies.
The persuasive effects of scarcity messages on impulsive buying in live-streaming e-commerce: the moderating role of time scarcity
Authors: Hao Shuaikang , Huang Ling 
Published: August 2024

PurposeLive-streaming e-commerce (LSE) allows anchors to bring offline promotion skills to interact with consumers and persuade them to buy. However, how consumers respond to these communications remains unknown. This study examines the persuasive effect of different scarcity marketing messages on impulsive buying in the LSE context.Design/methodology/approachThis study adopts scenario-based experimental methods and conducts two 2 quantity-based scarcity (supply-framed vs demand-framed)*2 time-based scarcity appeals (high vs low) between-subjects experiments to test the hypotheses.FindingsThe results indicate that supply-framed appeals are more effective in provoking consumers’ arousal and impulsive buying, but are moderated by time scarcity. Furthermore, emotional arousal only mediates the effects of quantity-based scarcity appeals on impulsive buying under high-level time scarcity conditions.Originality/valueThis study contributes to the e-commerce literature by comparing the persuasive effect of different scarcity messages in the LSE context. We broaden the scarcity marketing literature by testing the combined effect of quantity-based and time-based scarcity appeals. Finally, this study extends the application of the competitive arousal model.
The influence of culture, competence, and style of decision-making on the perceived adoption of evidence-based management sources among Polish managers
Authors: Cassar Vincent , Tracz-Krupa Katarzyna , Bezzina Frank 
Published: August 2024

PurposeIn this study, we explored factors driving evidence-based management (EBM) decision-making in Poland which has experienced changes from state-controlled market environments to more competitive ones. Evidence-based management requires the critical use and adaptability to information to deal with complex problems.Design/methodology/approachIn total, 422 Polish managers responded to a telephone survey measuring their perceptions about decision-making culture, styles, competence, and their use of specific sources to derive the evidence to enable them to make evidence-based decisions. Informed by theoretical principles, we used Hayes’ PROCESS macro (Model 4) to examine whether each factor produced direct effects on EBM decision-making and the mediating influence of competence and style in the relationship between culture and perceived evidence-based decision-making.FindingsAll three factors correlated positively with perceived evidence-based decision-making. Moreover, style was not predictive of EBM decision-making compared to competence and culture while culture had an imposing effect on decision-making both as a direct effect and indirectly through competence.Originality/valueThis study provides important insights into the perceptual state of EBM among Polish managers. It emphasizes the importance of embracing diverse cultures and improving critical thinking to help managers make more evidence-based decisions during significant changes in the business world.
Alternative to health consultations or safe spaces? Pregnant women’s health information-seeking behaviour online during the COVID-19 pandemic in Nigeria
Authors: Alade Moyosore , Sanusi Bernice 
Published: August 2024

Purpose
The COVID-19 pandemic disrupted healthcare systems globally, including antenatal care coverage. Pregnant women, who were considered “at risk” during the pandemic, replaced in-person antenatal visits with telemedicine and accessed health information online. However, little is known about pregnant women’s online information-seeking behaviour during the COVID-19 pandemic in Nigeria. Hence, the purpose of this paper is to investigate the information seeking behaviour of pregnant women online during the COVID-19 pandemic.

Design/methodology/approach
This research uses qualitative study and in-depth interviews to obtain data from eight pregnant women during the pandemic. Data were thematically analysed, with responses presented verbatim to illustrate themes.

Findings
Findings show that during the COVID-19 pandemic, the unavailability of health professionals and the fear of contracting the COVID-19 virus influenced pregnant women’s information-seeking behaviour online. Pregnant women accessed online sources as alternatives to consultations with health professionals, searched for drug prescriptions and asked pregnancy-related questions online. Findings also revealed that pregnant women conceptualised these online sources and platforms as safe spaces for sharing and dealing with pregnancy-related anxieties and difficulties during the pandemic.

Research limitations/implications
The number of participants sampled in the study is considered satisfactory since data saturation was achieved. However, considering the generalisation and transferability of the research findings, note that the study focused on a limited number of pregnant women in one state in Nigeria (Lagos State). Hence, the design and sample do not provide adequate generalisation to a larger population of pregnant women in Nigeria. Future research may generalise more broadly to other states in Nigeria. Another limitation of the study was using telephone interviews to collect data. Therefore, this paper could not analyse body language and facial expressions, which prevented us from gaining insights into participants’ descriptions of health information-seeking behaviour online. Therefore, further studies should use alternative data collection methods, such as face-to-face or online video interviews, instead of telephone interviews.

Practical implications
This study has implications for health policy interventions. The study’s findings can guide policies on designing digital health systems for pregnant women during health crises.

Originality/value
This study contributes to existing literature on health information-seeking behaviour online among a vulnerable population – pregnant women in a developing country. Specifically, the study contributes to knowledge on how pregnant women’s health information-seeking behaviour can change online within a health-crisis context like the COVID-19 pandemic and its implications for their overall well-being.
Does board gender diversity affect bank financial stability? Evidence from a transitional economy
Authors: Ho Trang N.T. , Nguyen Dat , Le Tu , Nguyen Hang Thanh , Tran Son 
Published: August 2024

Purpose
This study aims to investigate whether the changes in gender composition of bank board affects Vietnamese bank stability efficiency.

Design/methodology/approach
This research covers a panel of 27 commercial banks in Vietnam over a 14-year period from 2007 to 2020. The two-step system generalized method of moments is used to estimate the gender diversity–Vietnamese bank stability efficiency nexus.

Findings
The authors find that a greater degree of board gender diversification enhances bank stability efficiency and reduces bank risk-taking in Vietnam. The relationship between gender diversity and the stability efficiency of Vietnamese banks is still valid under the influence of regulatory capital sufficiency and during the financial crisis. These findings are robust to alternative proxies for risk indicators and consistent with the perspectives of stakeholder and behavior theory.

Originality/value
Although this research revisits the relationship between gender diversity and bank risk-taking, it is the first attempt to explore the role of women on board in enhancing the stability efficiency of banks, using the stochastic frontier approach. These findings shed light on the function of gender diversity as a governance instrument for mitigating risk in an emerging market context.
Exploring how institutionalized patriarchy relates to career outcomes among African women: evidence from Nigeria
Authors: Imhanrenialena Benedict Ogbemudia , Ebhotemhen Wilson , Agbaeze Emmanuel Kalu , Eze Nwafor Cletus , Oforkansi Ejike Sebastian 
Published: August 2024

Purpose
Following the renewed interest to harness the full potential of African female employees in the workplace, this paper aims to explore how patriarchal behaviors relate to career adaptability, subjective career success and job satisfaction among women in Nigerian organizations.

Design/methodology/approach
A structured questionnaire was used in collecting quantitative data from 508 middle-level managers in Nigerian organizations. The hypotheses were tested with structural equation modeling.

Findings
Patriarchal-induced gendered work practices were found to have a significant negative influence on career adaptability among Nigerian career women. Contrary to expectations, patriarchal discrimination was found to have an insignificant negative influence on job satisfaction and subjective career success, suggesting that Nigerian career women still experience significant subjective career success and job satisfaction amid patriarchal practices in the workplace.

Practical implications
For female employees to possess significant career adaptability resources that will enable them to reconstruct their careers to match redesigned job functions in times of innovation in the workplace, organizations should reinvent their human resources (HR) policies that address patriarchal-induced gendered work practices in the workplace.

Originality/value
This current study extends research on how patriarchy affects female employees in African organizations from the traditional research focus of patriarchy and work-life balance relationships to the under-explored area of career experience among women. To the best of the authors’ knowledge, this is the first quantitative research that explores how patriarchy influences career adaptability resources, subjective career success and job satisfaction among Nigerian female employees.