bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiological mechanisms of exercise related to prevention and treatment of breast cancer: A review
Authors: Koushkie Jahromi Maryam , Dehghani Ahmadreza , Ghasemi Mohammadhosein 
Published: August 2024

Background: Considering limitations related to intensity, duration, and mode of exercise and possible physiological mechanisms, this study aimed to summarize the physiological mechanisms emphasizing inflammation, angiogenesis, and immunology of different exercises related to prevention and treatment of breast cancer.
Methods: In the current review study, different platforms were analyzed including PubMed, Scopus, Cochrane, Embase, Web of Science, Science Direct, Google Scholar, and Research Gate as well as Iranian databases such as Scientific Information Database (SID) and Magiran. The data was reviewed until May 2024. Search keywords included “exercise", "sport”, “physical exercise”, “intensity, training”, “breast cancer”, “inflammation”, “angiogenesis”,” immunology”, and “physiological mechanisms”.
Results: Among several proposed mechanisms, inflammation, immunity and angiogenesis have been mentioned as important indices but there are ambiguities regarding the influence of different intensities and durations of exercise on breast cancer prevention and treatment. Other effective factors such as the effect of exercise on gene regulation and some other mechanisms have been proposed as possible mediators.
Conclusion: According to some previous studies, aerobic exercise induces positive effects on preventing and treating breast cancer through reducing inflammation, improving angiogenesis, and enhancing immunological mechanisms. Regarding duration and intensity, long term regular exercise (>8 weeks), in the form of aerobic and especially high intensity interval training (HIIT) reduces inflammation. while the effect of short-term exercise training is not clear yet and high intensity exercise may induce suppressing effects on the immune system. Therefore, the effect of intensity and duration of exercise on physiological mechanisms must be clarified.
BBSdb, an open resource for bacterial biofilm-associated proteins
Authors: Zhang Zhiyuan , Pan Yuanyuan , Hussain Wajid , Chen Guozhong , Li Erguang 
Published: August 2024

Bacterial biofilms are organized heterogeneous assemblages of microbial cells encased within a self-produced matrix of exopolysaccharides, extracellular DNA and proteins. Over the last decade, more and more biofilm-associated proteins have been discovered and investigated. Furthermore, omics techniques such as transcriptomes, proteomes also play important roles in identifying new biofilm-associated genes or proteins. However, those important data have been uploaded separately to various databases, which creates obstacles for biofilm researchers to have a comprehensive access to these data. In this work, we constructed BBSdb, a state-of-the-art open resource of bacterial biofilm-associated protein. It includes 48 different bacteria species, 105 transcriptome datasets, 21 proteome datasets, 1205 experimental samples, 57,823 differentially expressed genes (DEGs), 13,605 differentially expressed proteins (DEPs), 1,930 ‘Top 5% differentially expressed genes’, 444 ‘Threshold-based DEGs’ and a predictor for prediction of biofilm-associated protein. In addition, 1,781 biofilm-associated proteins, including annotation and sequences, were extracted from 942 articles and public databases via text-mining analysis. We used E. coli as an example to represent how to explore potential biofilm-associated proteins in bacteria. We believe that this study will be of broad interest to researchers in field of bacteria, especially biofilms, which are involved in bacterial growth, pathogenicity, and drug resistance.Availability and implementation: The BBSdb is freely available at http://124.222.145.44/#!/.
Effects of acute hypothyroidism on plasma melatonin and Aanat and Asmt expression in the pineal gland and gonads of rats
Authors: Paiva Rafaella Valete Nunes , Mondes Pedro Henrique de Lima , Brandão Beatriz de Jesus , Sant’Anna Julia Nascimento , Freire dos Santos Maria Eduarda , Fighera Yasmin Muniz , Santos Luciano Cardoso , Markus Regina P. , Fernandes Pedro Augusto Carlos Magno , Silva Juneo Freitas , Tamura Eduardo Koji 
Published: August 2024

IntroductionThe reproductive system is tightly regulated by environmental and physiological signals. Melatonin, known as the hormone of darkness, plays a crucial role in regulating both the circadian and reproductive systems in mammals. Hypothyroidism is a key endocrine disorder that harms the reproductive system. Despite many studies on melatonin’s effects on the reproductive system, there is conflicting information regarding melatonin synthesis modulation in hypothyroidism. The objective of this study was to investigate the modulation of plasma melatonin levels and gene expression of Aanat and Asmt in the pineal gland and gonads of rats with hypothyroidism at different times of the day.MethodsFemale and male Wistar rats were divided into control and hypothyroid groups. Hypothyroidism was induced using propylthiouracil (PTU) for 15 days, rats were euthanized six hours after lights on (ZT6), before lights off (ZT11.5), and six hours after lights off (ZT18). Free thyroxine (FT4) and melatonin were quantified in plasma, and gene expressions of melatonin synthesizing enzymes (Aanat and Asmt) were measured in pineal and sexual organs (testis and ovary). Also, morphological analysis was performed in sexual organs.ResultsThe results reveal some disparities between the sexes. Hypothyroidism reduced antral and primary follicles in the ovary, and reduced the weight of testis, epididymis, and prostate. In relation to gene expression, we observed a reduction in Aanat expression in the pineal gland during the light phase (ZT6), and in males, this reduction occurred during the dark phase (ZT18). Regarding Asmt expression, there was a decrease in females also during the dark phase (ZT18). In the gonads, there was an increase in expression in both sexes at ZT11.5. Additionally, it was interesting to observe the association between FT4 levels and Asmt expression in the gonads.ConclusionsThis study showed that acute hypothyroidism can affect components of the melatonergic system in gonads, particularly gene expression of melatonin synthesis enzymes (Aanat and Asmt) contributing to changes in reproduction organs during disease progression. These findings enhance our understanding of melatonin synthesis in the reproductive system during hypothyroidism, showing distinct responses in male and female rats, and suggest that hypothyroidism affects the circadian rhythmicity of melatonin synthesis in a sex-dependent manner.
Abnormal late postprandial glucagon response in type 1 diabetes is a function of differences in stimulated C-peptide concentrations
Authors: Zhang Lingyu , Qin Yao , Huang Yiting , Hu Qizhen , Wu Qian , Wang Xing , Zhang Mei 
Published: August 2024

BackgroundThe functional changes in alpha cells in patients with type 1 diabetes (T1D) with different residual beta cell functions remain poorly elucidated. The study aimed to investigate the relationship between glucagon secretion and C-peptide levels and to explore the relationship between glucagon response and glucose increment in respond to a secretagogue in a steamed bread meal tolerance test (BMTT) in T1D.MethodsThe study enrolled 43 adult patients with T1D and 24 healthy control subjects. Patients with T1D who underwent BMTT were divided into two groups based on peak C-peptide levels: C peptide low (CPL; C-peptide &lt; 200 pmol/L; n=14) and high (CPH; C peptide ≥ 200 pmol/L; n=29). Plasma glucose, C-peptide, glucagon levels at 0, 30, 60, 120, and 180 min were measured. The glucagon response to the BMTT was defined by areas under the curve (AUC) as early (AUC0-30), late (AUC30-180), or total (AUC0-180) glucagon.ResultsCompared to healthy individuals, fasting plasma glucagon was lower and postprandial plasma glucagon level was increased in patients with T1D. Glucagon levels after BMTT between the CPL and CPH group showed significant group by time interaction. Peak glucagon and glucagon at 60-180 min, total and late glucagon response were higher in CPL than CPH group, while fasting glucagon and early glucagon response adjusted for glucose were comparable between CPL and CPH group. The higher late glucagon response and late glucagon response adjusted for glucose were associated with lower peak C-peptide in T1D. The higher late glucagon response and lower peak C-peptide were associated with the higher value of ▵glucose at 180 min.ConclusionStimulated C-peptide levels affect the paradoxical increase in postprandial glucagon secretion in patients with T1D, especially late glucagon response. The exaggerated postprandial glucagon secretion further stimulates the elevation of postprandial glucose in patients with T1D.
Antenatal care service satisfaction and associated factors among pregnant women at public health facilities of Wogera district, Northwest Ethiopia: a cross-sectional study
Authors: Belachew Asrat Kassaw , Debie Ayal , Geberu Demiss Mulatu , Dagnew Abinet , Bitew Gedamnesh , Dejene Tadesse Mamo , Walle Agmasie Damtew 
Published: August 2024

BackgroundPregnant women's satisfaction with Ante-Natal Care (ANC) is crucial for improving its quality and providing standardized healthcare services. However, studies on pregnant women's satisfaction with antenatal care and associated factors are limited in Ethiopia, particularly in the study area. Therefore, this study aimed to assess satisfaction and associated factors among pregnant women receiving antenatal care in Wogera district.MethodsA facility-based cross-sectional study was conducted in Wogera district from March to April 2024. A total of 458 pregnant women who attended ANC at health facilities were included in the study. Interviewer -administered structured questionnaire was used to obtain the necessary information for this study and systematic random sampling was used to select the study participants. Multivariable and binary logistic regression analysis was used to identify the effect of each independent variable on the outcome (satisfaction).ResultsThe overall satisfaction of antenatal care services among pregnant women was 92.1% (95% CI: 89.5, 94.5%). The majority (98.3%) of them were satisfied by the provider's greeting and 97.8% were satisfied by the cost of service but pregnant women were less satisfied by waiting time to see the health workers, cleanness of the toilet, and water supply. Satisfaction of pregnant women was associated with housewife occupational status [AOR = 3.05, 95% CI: 1.02, 9.15], civil servants occupational status [AOR = 4.02, 95% CI: 1.02, 15.85], age ≥25 [AOR = 2.78, 95% CI: 1.05, 1.74], advice on family planning [AOR = 7.29, 95% CI: 3.08, 17.05], one ANC visit [AOR = 3.61, 95% CI: 1.84, 8.74] and the respondents pregnant women who have ≥2 pregnancy [AOR = 4.55, 95% CI: 1.88, 11.03] were the predictors of level of satisfaction.ConclusionPregnant women's satisfaction with antenatal care was high, influenced by factors such as having two or more pregnancies, timing of the first ANC visit, family planning advice, age ≥25 years, and being a housewife or civil servant. Efforts should focus on reducing wait times, ensuring clean water access, and improving latrine hygiene at healthcare facilities to sustain this satisfaction. Specifically, facilities should streamline appointments, maintain safe drinking water sources, and upgrade toilets for better comfort and hygiene.
Role of glycosylation-related gene MGAT1 in pancreatic ductal adenocarcinoma
Authors: Jiang Lai , Liu Jie , Zhang Shengke , Jiang Chenglu , Huang Jinbang , Chen Haiqing , Zhou Xuancheng , Fu Yiping , Yang Zhongqiu , Wang Rui , Yang Guanhu , Chi Hao , Li Bo 
Published: August 2024

Backgroundpancreatic ductal adenocarcinoma (PDAC) is a malignant tumor with a very poor prognosis and a complex tumor microenvironment, which plays a key role in tumor progression and treatment resistance. Glycosylation plays an important role in processes such as cell signaling, immune response and protein stability.Materials and methodssingle-cell RNA sequencing data and spatial transcriptome data were obtained from GSE197177 and GSE224411, respectively, and RNA-seq data and survival information were obtained from UCSC Xena and TCGA. Multiple transcriptomic data were comprehensively analyzed to explore the role of glycosylation processes in tumor progression, and functional experiments were performed to assess the effects of MGAT1 overexpression on PDAC cell proliferation and migration.ResultsIn PDAC tumor samples, the glycosylation level of macrophages was significantly higher than that of normal samples. MGAT1 was identified as a key glycosylation-related gene, and its high expression was associated with better patient prognosis. Overexpression of MGAT1 significantly inhibited the proliferation and migration of PDAC cells and affected intercellular interactions in the tumor microenvironment.ConclusionMGAT1 plays an important role in PDAC by regulating glycosylation levels in macrophages, influencing tumor progression and improving prognosis.MGAT1 is a potential therapeutic target for PDAC and further studies are needed to develop targeted therapeutic strategies against MGAT1 to improve clinical outcomes.
Attraction and avoidance of wild demersal fish and crustaceans to open-net aquaculture pens resolved by baited and towed underwater camera surveys
Authors: Dunlop Katherine , Strammer Ilona , Keeley Nigel 
Published: August 2024

Wild gadoids are known to have close associations with open-net fish farms, leading to concerns about changes in natural fish distribution and feeding patterns. However, the nature of the relationships between non-pelagic fish and mobile benthic invertebrates and aquaculture facilities remains poorly documented, even though this group contains widespread commercial North Atlantic species. This study utilizes baited remote and towed underwater video surveys to resolve the ecosystem-level effects of open salmon aquaculture cages and organic enrichment on the spatial structure and relative abundance of demersal fish and crustacean communities. Towed camera surveys were carried out 50 to &gt;600 m downstream of three farms producing finfish in Western Finnmark and four in the Frøya region of Norway. Towed camera surveys were paired with baited remote underwater video surveys at the four Frøya farms. The declining enrichment gradient with distance downstream of the farm cages was confirmed by modeled and observed depositional flux measurements and benthic infaunal community composition. The community structure of wild demersal fish and crustaceans in close association with the seafloor varied with the distance from the aquaculture sites. In addition to the established pattern of the attraction of gadoids, the edible crab (Cancer pagurus) and flatfish (Pleuronectes platessa and Microstomus kitt) showed a preference for areas 10–150 m from farm cages where organically enriched sediments contain a rich food source of opportunistic polychaetes. In contrast, the cuckoo wrasse (Labrus mixtus) and the common hermit crab (Pagurus bernhardus) exhibited higher numbers in locations over 600 m from the farms. Findings show how changes in the benthic food web from aquaculture enrichment can affect the spatial distribution of poorly studied but commercially important fish and crustaceans. Such changes in distribution can impact the availability of these species to coastal fisheries, especially in dense aquaculture regions.
Comparison of erythrocyte sedimentation rate measurement between Westergren method and automated method among patients attending Jigjiga University Sheik Hassen Yabare Referral Hospital, Jigjiga, Ethiopia
Authors: Walle Muluken , Alemayehu Ermiyas , Tesfaye Addisu , Arkew Mesay , Asmerom Haftu , Agidew Melaku Mekonnen , Getu Fasil 
Published: August 2024

IntroductionErythrocyte sedimentation rate (ESR) is a widely used screening test in clinical practice as an indicator of inflammatory and degenerative malignant diseases. The Westergren method, renowned as the gold standard, is valued for its accuracy and cost-effectiveness but demands considerable time and blood volume. Emerging automated methods offer quicker and more convenient alternatives, aiming to replace manual techniques. Nonetheless, validating these automated methods against the reference Westergren method is essential to ensure reliability. Therefore, this study aimed to evaluate ESR measurement results obtained from both the reference Westergren method and the automated (SFRI ESR 3000) method.MethodsA Hospital-based comparative cross-sectional study was conducted at Jigjiga University Sheik Hassen Yabare Referral Hospital from July 15 to September 16, 2023. Following the acquisition of informed consent, blood samples were obtained from 158 participants, five milliliters of blood from each participant. These samples were then subjected to ESR estimation using both the Westergren (reference) method and the automated (SFRI ESR 3000) method. Subsequently, the collected data were analyzed using SPSS version 20 and MedCalc version 12.3.0.0 statistical Softwares. Statistical analyses such as Paired t-tests, Pearson correlation, linear regression, and the Bland and Altman plot were employed. A p-value of &lt; 0.05 was considered statistically significant.ResultsThe paired sample t-test analysis revealed no significant difference between the use of the reference Westergren method and the automated method for ESR determination, with a mean difference (MD) of 0.7 ± 9.2 mm/h (P = 0.36). Additionally, a significant correlation was observed between the two methods, with a remarkable correlation coefficient (r = 0.94, p &lt; 0.001). The Bland–Altman data analysis indicated no evidence of systematic bias and demonstrated good agreement of ESR values between the two methods, with a limit of agreement of −17.3 to +18.7. Moreover, within-run imprecision analysis for the automated method across a range of ESR values showed coefficient of variation of 27.08, 12.65, and 10.32% for low, medium, and high ESR levels, respectively.ConclusionsThe SFRI ESR 300 automated method demonstrates the potential for interchangeable use with the Westergren method for determining ESR, given the strong correlation and good agreement. Additionally, the same reference range could be applied during interpretation.
Cerebellar white and gray matter abnormalities in temporal lobe epilepsy: a voxel-based morphometry study
Authors: Chen Yini , Pan Jingyu , Lin Andong , Sun Lu , Li Yufei , Lin Hongsen , Pu Renwang , Wang Ying , Qi Yiwei , Sun Bo 
Published: August 2024

BackgroundPrevious structural neuroimaging studies linked cerebellar deficits to temporal lobe epilepsy (TLE). The functions of various cerebellar regions are increasingly being valued, and their changes in TLE patients warrant further in-depth investigation. In this study, we used the Spatially Unbiased Infratentorial (SUIT) toolbox with a new template to evaluate the cerebellar structural abnormalities in patients with TLE, and further explored the relationship between the changes of different cerebellar regions and cognition.MethodsThirty-two patients with TLE were compared with 39 healthy controls (HC) matched according to age, gender, handedness, and education level. All participants underwent a high-resolution T1-weighted MRI scan on a 3.0 Tesla scanner. We used a voxel-based morphometry (VBM) approach utilizing the SUIT toolbox to provide an optimized and fine-grained exploration of cerebellar structural alterations associated with TLE.ResultsCompared with HC, TLE patients showed a significant reduction in the volume of gray matter in the Left lobule VI and white matter in the Right Crus II. In the TLE patient group, we conducted partial correlation analysis between the volumes of different cerebellar regions and cognitive rating scale scores, such as MMSE and MoCA. The volume of the Left lobule VI (GM) exhibited a positive correlation with the MMSE score, but no significant correlation was found with the MoCA score. Furthermore, it was observed that the MMSE was more effective than the MoCA in identifying epilepsy patients with cognitive impairment.ConclusionThis study supported previous research indicating that temporal lobe epilepsy (TLE) is linked to structural changes in the cerebellum, specifically affecting the volume of gray matter. These findings offer valuable insights into the neurobiology of TLE and hold potential to inform the development of enhanced diagnostic methods and more effective treatment approaches.
Human reasoning on social interactions in ecological contexts: insights from the theory of mind brain circuits
Authors: Isernia Sara , Pirastru Alice , Rossetto Federica , Cacciatore Diego Michael , Cazzoli Marta , Blasi Valeria , Baksh R. Asaad , MacPherson Sarah E. , Baglio Francesca 
Published: August 2024

IntroductionThe relationship between neural social cognition patterns and performance on social cognition tasks in daily life is a topic of debate, with key consideration given to the extent to which theory of mind (ToM) brain circuits share properties reflecting everyday social functioning. To test the efficacy of ecological stimuli in eliciting brain activation within the ToM brain circuits, we adapted the Edinburgh Social Cognition test social scenarios, consisting of dynamic ecological contextually embedded social stimuli, to a fMRI paradigm.MethodsForty-two adults (21 men, mean age ± SD = 34.19 years ±12.57) were enrolled and underwent an fMRI assessment which consisted of a ToM task using the Edinburgh Social Cognition test scenarios. We used the same stimuli to prompt implicit (movie viewing) and explicit (silent and two-choice answers) reasoning on cognitive and affective mental states. The fMRI analysis was based on the classical random effect analysis. Group inferences were complemented with supplemental analyses using overlap maps to assess inter-subject variability.ResultsWe found that explicit mentalizing reasoning yielded wide neural activations when two-choice answers were used. We also observed that the nature of ToM reasoning, that is, affective or cognitive, played a significant role in activating different neural circuits.DiscussionThe ESCoT stimuli were particularly effective in evoking ToM core neural underpinnings and elicited executive frontal loops. Future work may employ the task in a clinical setting to investigate ToM network reorganization and plasticity.