bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isoliquiritigenin‐induced effects on Nrf2 mediated antioxidant defence in the HL‐60 cell monocytic differentiation
Authors: Chen Hongmei , Zhang Bo , Yuan Xuan , Yao Ying , Zhao Hong , Sun Xiling , Zheng Quisheng 
Published: August 2013

AbstractTo evaluate the role of redox homeostasis in differentiation in human promyelocytic leukemia cells (HL‐60) induced by isoliquiritigenin (ISL) through modulation of the nuclear erythroid‐related factor 2/antioxidant responsive element (Nrf2/ARE) pathway. Morphological changes, cell surface markers CD11b/CD14, and nitroblue tetrazolium (NBT)‐reducing ability were used to determine the differentiation of HL‐60, and 2,7‐dichlorofluorescein was used to detect the level of intracellular reactive oxygen species (ROS). Thiobarbituric acid test was utilised to determine the levels of malondialdehyde production in ISL‐treated HL‐60. The study determines and presents the redox state of the ratio of reduced/oxidised glutathione as a consequence of progression from differentiation in HL‐60. Expression levels of the Nrf2/ARE downstream target genes were determined by quantitative polymerase chain reaction. Nicotinamide adenine dinucleotide phosphate‐oxidase (NADPH oxidase) inhibitors, apocynin (APO), and diphenyleneiodonium (DPI) were used for the preliminary study to determine the potential downstream targets regulated by NADPH oxidase in ISL‐induced HL‐60 differentiation. The data showed a strong dose–response relationship between ISL exposure and the characteristics of HL‐60 differentiation, namely, morphology changes, NBT reductive activities, and expression levels of surface antigens CD11b/CD14. Intercellular redox homeostasis changes toward oxidation during drug exposure are necessary to support ISL‐induced differentiation. The unique expression levels of the Nrf2/ARE downstream target genes in the differentiation of HL‐60 recorded a statistically significant and dose‐dependent increase (P < 0.05), which were suppressed by NADPH oxidase inhibitor, APO, and DPI. ISL as a differentiation‐inducing agent with mechanisms involved in the Nrf2/ARE pathway to modulate intercellular redox homeostasis, and thus, facilitate differentiation.
Combination of BMP‐2 and 5‐AZA is advantageous in rat bone marrow‐derived mesenchymal stem cells differentiation into cardiomyocytes
Authors: Hou Jing , Lü An‐Lin , Liu Bo‐Wu , Xing Yu‐Jie , Da Jing , Hou Zhao‐Lei , Ai Shi‐Yi 
Published: August 2013

AbstractBone morphogenetic protein‐2 (BMP‐2) has a crucial role in the development of cardiogenesis, and is used in inducing bone marrow mesenchymal stem cells (BMMSCs) to differentiate into cardiomyocytes. We have examined a combination of BMP‐2 and 5‐azacytidine (5‐AZA) in inducing these differentiation effects. BMMSCs were collected and purified from bone marrow of 4‐week‐old Sprague‐Dawley (SD) rats by density‐gradient centrifugation and differential attachment. The fourth passage subculture of BMMSCs, selected by cytometry for purity and identification, was divided into four groups: a control group, BMP‐2 treated, 5‐AZA treated, and a combination of BMP‐2 and 5‐AZA treatment. Expression of cardiac Troponin I (cTnI) and Connexin 43 (CX‐43) in BMMSCs after induction were detected by immunofluorescence and Western blot. Flow cytometry analysis was used for differentiation rates and apoptosis of induced BMMSCs, through the expression of cardiac Troponin T (cTnT) and Annexin V‐FITC & PI kit, respectively. BMP‐2 can ameliorate apoptosis of BMMSCs caused by 5‐AZA and promote the differentiation of BMMSCs into cardiomyocyte‐like cells. Thus a combination of BMP‐2 and 5‐AZA can significantly improve the cardiac differentiation with fewer cell damage effects, making it a safe and effective method of induction in vitro.
The lectin of Dolichos biflorus agglutinin recognises glycan epitopes on the surface of a subset of cardiac progenitor cells
Authors: Chen Zhanfeng , Wang Man , Xiang Qiang , Sun Zhenliang , Zhang Rong 
Published: August 2013

AbstractThe discovery of adult cardiac progenitor cells (CPCs) provides a promising way for treating heart disease; however, their surface characteristics that play a critical role in regulating their maintenance, self‐renewal, migration, and differentiation have not been fully investigated. One subpopulation of Dolichos biflorus agglutinin (DBA)‐positive cells was identified in the heart of adult mice. Flow cytometry showed that 3.7% of heart cells could be labeled by FITC conjugated DBA. BrdU pulse‐chase showed that 55–75% of DBA+ cells were CPCs. Evidences from 5‐FU‐induced myelosuppression along with BrdU pulse‐chasing suggests that DBA‐positive cells are proliferative. Furthermore, DBA positive cells display a cologenic appearance in vivo. Our findings suggest that DBA‐positive cells in the heart of adult mouse contained a subset of CPCs, and DBA reactivity is one novel surface characteristic on CPCs.
Optimization of a Multiple Impinging Jets Cavitation Reactor Using Zero‐Valent Iron Powder as Catalyst
Authors: Basiri Parsa J. , Ebrahimzadeh Zonouzian S. A. 
Published: August 2013

AbstractThe effect of a combination of impinging jets and hydrodynamic cavitation technology was investigated by a multiple impinging jets cavitation reactor (MIJCR). Zero‐valent iron (ZVI) powder and rhodamine B were chosen as heterogeneous catalyst and model pollutant, respectively. ZVI addition resulted in more than five times higher decolorization efficiency compared to runs without ZVI. In situ generation of Fenton reagents suggested an advanced Fenton process to explain the enhancing effect of the applied catalyst. Impinging jets and aeration led to significant enhancement of the process, and optimum values of inter‐nozzle distance and initial catalyst concentration favored the decolorization of rhodamine B.
Multitasking Water‐Soluble Synthetic G‐Quartets: From Preferential RNA–Quadruplex Interaction to Biocatalytic Activity
Authors: Haudecoeur Romain , Stefan Loic , Monchaud David 
Published: August 2013

AbstractNatural G‐quartets, a cyclic and coplanar array of four guanine residues held together through a Watson–Crick/Hoogsteen hydrogen‐bond network, have received recently much attention due to their involvement in G‐quadruplex DNA, an alternative higher‐order DNA structure strongly suspected to play important roles in key cellular events. Besides this, synthetic G‐quartets (SQ), which artificially mimic native G‐quartets, have also been widely studied for their involvement in nanotechnological applications (i.e., nanowires, artificial ion channels, etc.). In contrast, intramolecular synthetic G‐quartets (iSQ), also named template‐assembled synthetic G‐quartets (TASQ), have been more sparingly investigated, despite a technological potential just as interesting. Herein, we report on a particular iSQ named PNADOTASQ, which demonstrates very interesting properties in terms of DNA and RNA interaction (notably its selective recognition of quadruplexes according to a bioinspired process) and catalytic activities, through its ability to perform peroxidase‐like hemin‐mediated oxidations either in an autonomous fashion (i.e., as pre‐catalyst for TASQzyme reactions) or in conjunction with quadruplex DNA (i.e., as enhancing agents for DNAzyme processes). These results provide a solid scientific basis for TASQ to be used as multitasking tools for bionanotechnological applications.
Highly Efficient Construction of Bisspirooxindoles Containing Vicinal Spirocenters through an Organocatalytic Modified Feist–Bénary Reaction
Authors: Ahadi Somayeh , Khavasi Hamid Reza , Bazgir Ayoob 
Published: August 2013

AbstractWe have developed an organocatalytic modified Feist–Bénary reaction of cyclic dicarbonyl compounds, isatins and cyclic α‐bromo dicarbonyl compounds. This method affords bisspirooxindole‐fused dihydrofurans containing two vicinal spiro centers. To the best of our knowledge, employing cyclic α‐halo dicarbonyl compounds for the synthesis of bisspirooxindole‐fused dihydrofurans has not been previously reported.
A Combined Experimental and Computational Study of Linear Ruthenium(II) Coordination Oligomers with End‐Capping Organic Redox Sites: Insight into the Light Absorption and Charge Delocalization
Authors: Yao Chang‐Jiang , Zheng Ren‐Hui , Nie Hai‐Jing , Cui Bin‐Bin , Shi Qiang , Yao Jiannian , Zhong Yu‐Wu 
Published: August 2013

AbstractTwo series of linear ruthenium coordination oligomers, [(Ntpy)Run(tppz)n−1(tpy)]2n+ (mono‐Ntpy series, n=1–3) and [(Ntpy)2Run(tppz)n−1]2n+ (bis‐Ntpy series, n=1–3) have been prepared, where Ntpy is the capping ligand 4′‐di‐p‐anisylamino‐2,2′:6′,2′′‐terpyridine, tppz is tetra‐2‐pyridylpyrazine, and tpy is 2,2′:6′,2′′‐terpyridine. The electrochemical measurements evidence oxidation events from both the amine segments and the metal centers and reduction waves from tppz and the capping ligands. Both series complexes display much enhanced light absorption with respect to model complexes without terminal amine units. Density functional theory (DFT) calculations have been performed on both series and time‐dependent DFT (TD‐DFT) calculations have been performed on the bis‐Ntpy‐series compounds (n=1–4) to characterize their electronic structures and excited states and predict the electronic properties of long‐chain polymers. Upon one‐electron oxidation, the mono‐Ntpy‐series monoruthenium and diruthenium complexes display N+‐localized transitions and metal‐to‐nitrogen charge‐transfer (MNCT) transitions in the near‐infrared (NIR) region. DFT and TD‐DFT computations on the one‐electron‐oxidized forms of the mono‐Ntpy‐series compounds (n=1–4) provide insight into the nature of the MNCT transitions and the degree of charge delocalization.
Donor–Acceptor (D–A)‐Substituted Polyyne Chromophores: Modulation of Their Optoelectronic Properties by Varying the Length of the Acetylene Spacer
Authors: Štefko Martin , Tzirakis Manolis D. , Breiten Benjamin , Ebert Marc‐Olivier , Dumele Oliver , Schweizer W. Bernd , Gisselbrecht Jean‐Paul , Boudon Corinne , Beels Marten T. , Biaggio Ivan , Diederich François 
Published: August 2013

AbstractA series of donor–acceptor‐substituted alkynes, 2 a–f, was synthesized in which the length of the π‐conjugated polyyne spacer between the N,N‐diisopropylanilino donor and the 1,1,4,4‐tetracyanobuta‐1,3‐diene (TCBD) acceptor was systematically changed. The effect of this structural change on the optoelectronic properties of the molecules and, ultimately, their third‐order optical nonlinearity was comprehensively investigated. The branched N,N‐diisopropyl groups on the anilino donor moieties combined with the nonplanar geometry of 2 a–f imparted exceptionally high solubility to these chromophores. This important property allowed for performing INADEQUATE NMR measurements without 13C labeling, which, in turn, resulted in a complete assignment of the carbon skeleton in chromophores 2 a–f and the determination of the 13C–13C coupling constants. This body of data provided unprecedented insight into characteristic 13C chemical shift patterns in push–pull‐substituted polyynes. Electrochemical and UV/Vis spectroscopic studies showed that the HOMO–LUMO energy gap decreases with increasing length of the polyyne spacer, while this effect levels off for spacers with more than four acetylene units. The third‐order optical nonlinearity of this series of molecules was determined by measuring the rotational averages of the third‐order polarizabilities (γrot) by degenerate four‐wave mixing (DFWM). These latter studies revealed high third‐order optical nonlinearities for the new chromophores; most importantly, they provided fundamental insight into the effect of the conjugated spacer length in D–A polyynes, that can be exploited in the future design of suitable charge‐transfer chromophores for applications in optoelectronic devices.
19F NMR Spectroscopy as a Probe of Cytoplasmic Viscosity and Weak Protein Interactions in Living Cells
Authors: Ye Yansheng , Liu Xiaoli , Zhang Zeting , Wu Qiong , Jiang Bin , Jiang Ling , Zhang Xu , Liu Maili , Pielak Gary J. , Li Conggang 
Published: August 2013

AbstractProtein mobility in living cells is vital for cell function. Both cytosolic viscosity and weak protein–protein interactions affect mobility, but examining viscosity and weak interaction effects is challenging. Herein, we demonstrate the use of 19F NMR spectroscopy to measure cytoplasmic viscosity and to characterize nonspecific protein–protein interactions in living Escherichia coli cells. The origins of resonance broadening in Escherichia coli cells were also investigated. We found that sample inhomogeneity has a negligible effect on resonance broadening, the cytoplasmic viscosity is only about 2–3 times that of water, and ubiquitous transient weak protein–protein interactions in the cytosol play a significant role in governing the detection of proteins by using in‐cell NMR spectroscopy.
Control of Exchange Interactions in π Dimers of 6‐Oxophenalenoxyl Neutral π Radicals: Spin‐Density Distributions and Multicentered–Two‐Electron Bonding Governed by Topological Symmetry and Substitution at the 8‐Position
Authors: Nishida Shinsuke , Kawai Junya , Moriguchi Miki , Ohba Tomohiro , Haneda Naoki , Fukui Kozo , Fuyuhiro Akira , Shiomi Daisuke , Sato Kazunobu , Takui Takeji , Nakasuji Kazuhiro , Morita Yasushi 
Published: August 2013

AbstractThe tri‐tert‐butylphenalenyl (TBPLY) radical exists as a π dimer in the crystal form with perfect overlapping of the singly occupied molecular orbitals (SOMOs) causing strong antiferromagnetic exchange interactions. 2,5‐Di‐tert‐butyl‐6‐oxophenalenoxyl (6OPO) is a phenalenyl‐based air‐stable neutral π radical with extensive spin delocalization and is a counter analogue of phenalenyl in terms of the topological symmetry of the spin density distribution. X‐ray crystal structure analyses showed that 8‐tert‐butyl‐ and 8‐(p‐XC6H4)‐6OPOs (X=I, Br) also form π dimers in the crystalline state. The π‐dimeric structure of 8‐tert‐butyl‐6OPO is seemingly similar to that of TBPLY even though its SOMO–SOMO overlap is small compared with that of TBPLY. The 8‐(p‐XC6H4) derivatives form slipped stacking π dimers in which the SOMO–SOMO overlaps are greater than in 8‐tert‐butyl‐6OPO, but still smaller than in TBPLY. The solid‐state electronic spectra of the 6OPO derivatives show much weaker intradimer charge‐transfer bands, and SQUID measurements for 8‐(p‐BrC6H4)‐6OPO show a weak antiferromagnetic exchange interaction in the π dimer. These results demonstrate that the control of the spin distribution patterns of the phenalenyl skeleton switches the mode of exchange interaction within the phenalenyl‐based π dimer. The formation of the relevant multicenter–two‐electron bonds is discussed.
Cyclisation versus 1,1‐Carboboration: Reactions of B(C6F5)3 with Propargyl Amides
Authors: Melen Rebecca L. , Hansmann Max M. , Lough Alan J. , Hashmi A. Stephen K. , Stephan Douglas W. 
Published: August 2013

AbstractA series of propargyl amides were prepared and their reactions with the Lewis acidic compound B(C6F5)3 were investigated. These reactions were shown to afford novel heterocycles under mild conditions. The reaction of a variety of N‐substituted propargyl amides with B(C6F5)3 led to an intramolecular oxo‐boration cyclisation reaction, which afforded the 5‐alkylidene‐4,5‐dihydrooxazolium borate species. Secondary propargyl amides gave oxazoles in B(C6F5)3 mediated (catalytic) cyclisation reactions. In the special case of disubstitution adjacent to the nitrogen atom, 1,1‐carboboration is favoured as a result of the increased steric hindrance (1,3‐allylic strain) in the 5‐alkylidene‐4,5‐dihydrooxazolium borate species.
Copper‐Mediated Deoxygenative Trifluoromethylation of Benzylic Xanthates: Generation of a CCF3 Bond from an O‐Based Electrophile
Authors: Zhu Lingui , Liu Shasha , Douglas Justin T. , Altman Ryan A. 
Published: August 2013

AbstractThe conversion of an alcohol‐based functional group, into a trifluoromethyl analogue is a desirable transformation. However, few methods are capable of converting O‐based electrophiles into trifluoromethanes. The copper‐mediated trifluoromethylation of benzylic xanthates using Umemoto’s reagent as the source of CF3 to form CCF3 bonds is described. The method is compatible with an array of benzylic xanthates bearing useful functional groups. A preliminary mechanistic investigation suggests that the CCF3 bond forms by reaction of the substrate with in situ generated CuCF3 and CuOTf. Further evidence suggests that the reaction could proceed via a radical cation intermediate.
Fractional Countercurrent Crystallization for the Production of Highly Purified Pharmaceuticals
Authors: Temmel Erik , Wloch Sebastian , Müller Uwe , Grawe Detlef , Eilers Robert , Lorenz Heike , Seidel‐Morgenstern Andreas 
Published: August 2013

AbstractBei Verunreinigungen, die partielle feste Lösungen mit der Zielsubstanz bilden, sind bei der Aufreinigung gebräuchlichen Kristallisationsverfahren Grenzen gesetzt. Für dieses Problem stellt die fraktionierende Kristallisation eine Alternative dar. In diesem Beitrag soll die Anwendung einer Kristallisationskaskade zur Herstellung eines hochreinen Wirkstoffs gezeigt werden. Durch den ausschließlichen Transport flüssiger Phasen kann dieser Prozess automatisch betrieben, ein Gegenstrom zwischen den Phasen hergestellt und dadurch die Effizienz der fraktionierenden Kristallisation deutlich gesteigert werden. Es werden die Massenbilanzen für den mehrstufigen Prozess hergeleitet und die Ergebnisse von Simulationsstudien mit einer experimentellen Fallstudie verglichen.
Exceptional Adsorption and Separation Properties of the Molecular Sieve ZIF‐8
Authors: Böhme Ulrike , Paula Carolin , Reddy Marthala V. R. , Caro Jürgen , Hartmann Martin 
Published: August 2013

AbstractDie Trennung von Olefin/Paraffin‐Gemischen erfolgt nach derzeitigem Stand der Technik durch Tieftemperaturrektifikation. Eine Alternative zu diesem energie‐ und kostenintensiven Verfahren könnte die Adsorption an mikroporösen Koordinationspolymeren sein. Insbesondere zeolithische Imidazolatnetzwerke, beispielsweise das hier untersuchte ZIF‐8, zeigen in dieser Hinsicht interessante Eigenschaften, da die Alkane gegenüber den Alkenen bevorzugt adsorbiert werden. Die im Rahmen dieser Arbeit aufgenommenen Reinstoffadsorptionsisothermen und Durchbruchsexperimente für C2‐ bis C4‐Kohlenwasserstoffe demonstrieren das Potenzial dieses Adsorbens für die Olefin/Paraffin‐Trennung.
Effect of prophylactic cranial irradiation on survival in elderly patients with limited‐stage small cell lung cancer
Authors: Eaton Bree R. , Kim Sungjin , Marcus David M. , Prabhu Roshan , Chen Zhengjia , Ramalingam Suresh S. , Curran Walter J. , Higgins Kristin A. 
Published: August 2013

BACKGROUNDProphylactic cranial irradiation (PCI) improves survival in patients with limited‐stage small cell lung cancer (SCLC) who have a complete response to chemotherapy and radiotherapy, yet to the best of the authors' knowledge, data specific to the elderly population are lacking.METHODSUsing the Surveillance, Epidemiology, and End Results (SEER) database, the authors identified 1926 patients aged ≥ 70 years who were diagnosed with limited‐stage SCLC between 1988 and 1997. Overall survival (OS) for patients who received PCI versus those who did not were estimated using the Kaplan‐Meier method and compared with the log‐rank test. A Cox proportional hazards model was further fitted to estimate the effect of PCI on OS after adjusting for age, race, sex, tumor size, lymph node status, stage of disease, and receipt of thoracic radiotherapy and surgery.RESULTSThe median age of the patients was 75 years (range, 70 years‐94 years) and 138 patients (7.2 %) received PCI. The 2‐year and 5‐year OS rates were 33.3% (95% confidence interval [95% CI], 25.6%‐41.2%) and 11.6% (95% CI, 6.9%‐17.6%), respectively, among patients who received PCI versus 23.1% (95% CI, 21.2%‐25.1%) and 8.6% (95% CI, 7.3%‐9.9%), respectively, among patients who did not receive PCI (P = .028). On multivariable analysis, PCI was found to be an independent predictor of OS (hazards ratio, 0.72; 95% CI, 0.54‐0.97 [P = .032]). On subgroup analysis, PCI remained an independent predictor of OS among patients aged ≥ 75 years, but not among patients aged ≥ 80 years.CONCLUSIONSThe receipt of PCI is associated with improved OS in patients aged ≥ 70 years with SCLC, suggesting that the benefit of PCI is maintained in the elderly population. Cancer 2013;119:3753–3760. © 2013 American Cancer Society.
Efficacy and safety profile of long‐term exposure to lenalidomide in patients with recurrent multiple myeloma
Authors: Fouquet Guillemette , Tardy Stéphanie , Demarquette Hélène , Bonnet Sarah , Gay Julie , Debarri Houria , Herbaux Charles , Guidez Stéphanie , Michel Jessica , Perrot Aurore , Serrier Caroline , Miljkovic Darko , Avet Loiseau Hervé , Facon Thierry , Hulin Cyrille , Leleu Xavier 
Published: August 2013

BACKGROUNDLenalidomide in combination with dexamethasone (Len/Dex) is indicated for patients with recurrent/refractory multiple myeloma (RRMM) who were treated with 1 prior therapy until evidence of disease progression. The objective of the current study was to determine the efficacy and safety profile of long‐term exposure to Len/Dex.METHODSA total of 50 patients with RRMM who were treated with long‐term Len for ≥ 2 years from 2 Intergroupe Francophone du Myélome (IFM) centers (Lille and Nancy) were included in the current study.RESULTSThe median age of the patients was 58 years, with 30% of the patients aged > 65 years, 49% having an International Staging System stage of 2 and 3, 12% having severe renal insufficiency, and 8% demonstrating an adverse result on fluorescence in situ hybridization. Approximately 56% of the patients received treatment with Len/Dex for ≥ 3 years. The median duration of treatment with Len/Dex was 3 years (range, 2 years‐7 years). The response rates for partial response or better and very good partial response or better for the overall cohort were 96% and 74%, respectively, which is similar to patients exposed to Len for ≥ 3 years. With a median follow‐up of 4 years, 19 (38%) patients had stopped treatment with Len/Dex. The time to disease progression rate at 37 months was 78% and 91%, respectively, in patients exposed to Len for 2 years to < 3 years and for ≥ 3 years (P = 025). The safety profile was manageable, similar to that of Len when administered for a shorter period of time; 16% of patients had grade 3 to 4 neutropenia, 6% had thrombopenia, 6% had anemia, and 20% experienced thromboembolic events, all of venous type. The annual incidence rate of second primary malignancy was 1.96% in the current series.CONCLUSIONSThe results of the current study confirmed that the Len/Dex combination is feasible for long‐term use in patients with RRMM, with a significant benefit noted in terms of time to disease progression for prolonged treatment with Len/Dex. Cancer 2013;119:3680–3686. © 2013 American Cancer Society.