bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Reference Genome Sequence ofSaccharomyces cerevisiae: Then and Now
Authors: Engel Stacia R , Dietrich Fred S , Fisk Dianna G , Binkley Gail , Balakrishnan Rama , Costanzo Maria C , Dwight Selina S , Hitz Benjamin C , Karra Kalpana , Nash Robert S , Weng Shuai , Wong Edith D , Lloyd Paul , Skrzypek Marek S , Miyasato Stuart R , Simison Matt , Cherry J Michael 
Published: January 2014

AbstractThe genome of the budding yeast Saccharomyces cerevisiae was the first completely sequenced from a eukaryote. It was released in 1996 as the work of a worldwide effort of hundreds of researchers. In the time since, the yeast genome has been intensively studied by geneticists, molecular biologists, and computational scientists all over the world. Maintenance and annotation of the genome sequence have long been provided by the Saccharomyces Genome Database, one of the original model organism databases. To deepen our understanding of the eukaryotic genome, the S. cerevisiae strain S288C reference genome sequence was updated recently in its first major update since 1996. The new version, called “S288C 2010,” was determined from a single yeast colony using modern sequencing technologies and serves as the anchor for further innovations in yeast genomic science.
Creating associative memory distortions - a Polish adaptation of the DRM paradigm
Authors: Ulatowska Joanna , Olszewska Justyna 
Published: January 2014

Abstract
One of the most widely applied techniques used to examine associative memory errors is the Deese-Roediger- McDermott (DRM) paradigm. The aim of the present studies was to demonstrate a Polish version of the DRM paradigm and to test the characteristics of memory illusions evoked by this procedure for both recall and recognition. A normative study was conducted to prepare Polish stimuli material sharing similar characteristics as the lists in the English language version. Subsequently, the lists were applied to examine the effect of prior recall on recognition, as well as the influence of retention interval on recall. The results revealed that the Polish version of the DRM paradigm induced a robust effect of false recall and recognition. Moreover, it was revealed that immediate recall of a single list led to a higher rate of both correct and false recall and that prior recall positively influenced recognition, leading to a higher rate of hits.
Depression among the Geriatric Population is a Matter of Concern: A Community Based Study in a Rural Area of West Bengal
Authors: Dasgupta A , Ray D , Roy S , Sarkar T , Ghosal A , Das A , Pal J 
Published: January 2014

Background Depression is commonest psychiatric disorder in elderly; it is often misdiagnosed and undertreated. People with depression suffer from impairment of all major areas of functioning ranging from personal care to social capability, which in turn decreases individuals’ quality of life and increases dependency on others Elderly patients with depression are more prone in developing chronic diseases like ischemic heart disease, diabetes mellitus, hypertension. This problem may be more severe in rural population. Objective of this study is to find out the magnitude of depression and its correlates among a rural geriatric population. Materials and Methods Population based cross sectional study was done in Singur, West Bengal with a sample size of 85 with the help of a pretested semi structured schedule and a geriatric depression scale (short form). Results Majority 61.2(%) of the study population were in the age group of 60 to 69 years and most belonged to joint Hindu family. Cardiovascular disease (67.05%) especially hypertension followed by musculoskeletal problems (42.35%) were the two major health related conditions suffered by this population and they usually made avail of government health facilities during illness. About 58.8% of the population suffered from depression and this was associated with nuclear family AOR(95%CI)=8.625(1.697-43.831), with poor income AOR(95%CI)=7.628(1.862-31.847), financial dependence AOR(95%CI)=4.948 (1.044-23.554)and living without spouse AOR(95%CI)=7.422 (1.513-36.418) and also presence of disease OR =5.423 (1.348-21.81). Conclusion Alarmingly, the prevalence of depression was observed to be quite high in this rural population and so was prevalence of different morbidities. All these will hasten the isolation of this vulnerable population from general community and increase burden on younger generation. DOI: http://dx.doi.org/10.3126/nje.v3i4.9515 Keywords:
Low Molecular Weight Heparin versus Aspirin plus Intermittent Compression Devices for Thromboprophylaxis in Indian Patients Undergoing Total Hip and Knee Arthroplasty
Authors: Paudel Sushil 
Published: January 2014

Introduction: Thromboembolic complications is common after arthroplasty. The purpose of this study was to find out the incidences of deep vein thrombosis, symptomatic, and fatal pulmonary embolism in Indian patients undergoing arthroplasties and to compare the effectiveness and safety of thromboprophylaxis with low molecular weight heparin versus aspirin plus intermittent pneumatic compression device. Methods: Patients who had a total hip and knee arthroplasty were randomized to receive prophylaxis with LMWH or aspirin plus intermittent pneumatic compression device. After four to six days, all patients underwent bilateral lower-extremity color Doppler ultrasonography to screen for deep venous thrombi in the calf and thigh. Any clinical symptoms of pulmonary embolism were evaluated with pulmonary CT angiography. Bleeding events in both groups were documented. The patients were followed up at 6 week and 3 months with Color Doppler to look for the evidence of deep venous thrombi. Results: 300 patients (409 joints) were randomized into 2 groups and studied regarding the incidence of deep vein thrombosis, pulmonary embolism, safety of the thromboprophylaxis in regard to its efficacy. Demographics were similar clinically between the groups. The rate of major bleeding events was 0.67% in the aspirin and compression group and 6% in the low-molecular-weight heparin group. Overall, prevalence of DVT and PE in Indian patients who underwent total hip or knee arthroplasty was 0.67% and 0.33% respectively. The rates of deep venous thrombosis were 1.33%, in the aspirin plus compression group compared 0% in the LMWH group. The rates of pulmonary embolism were 0.67% in the Aspirin plus compression group and 0% in the heparin group, and there were no fatal pulmonary emboli. Within the six week and three month follow-up period, no events occurred. There was no difference between the groups with regard to the prevalence of venous thromboembolism. Conclusions: An inexpensive multimodal protocol, consisting of aspirin, exercises, and the use of intermittent compression devices, was associated with relatively higher thromboembolic complications. However, major bleeding events were significantly lower in Aspirin group. DOI: http://dx.doi.org/10.3126/noaj.v3i2.9525 NOAJ July-December 2013, Vol 3, Issue 2, 26-32
Conservative treatment of TB Spondylitis in Dorsolumbar and Lumbar spine
Authors: Pradhan Rabindra Lal , Pandey Bimal Kumar , Sharma Sashmit , Lakhey Shishir , Manadhar Rajiv Raj , Rijal Kiran Prasad , Prasai Tanup 
Published: January 2014

Introduction: Tuberculosis (TB) is endemic in South Asia including Nepal. TB spine is the most common musculoskeletal manifestations and can have devastating complications. Early diagnosis by proper history, physical and radiological examination is mandatory. Chemotherapy is effective in most cases while some require surgical treatment. Methods: Forty-four patients with clinico-radiological evidence of TB were treated with four drug regime for 18 months and all patients were followed up till the end of the chemotherapy. Results: All 44 patients (M-28/ F-16) had back pain not responding to analgesics and physiotherapy while few had constitutional symptoms. Localized tenderness and paravertebral muscle spasm was present in 32/44 (72.7%) and the rest had palpable swelling at the dorsolumbar and lumbar regions. According to the classification by Oguz et al. the majority of the patients were in the Type I B. Six patients who did not show any improvement in pain and resolution of swelling were admitted and two more drugs (Steptomycin and Ofloxacin) were added. Conclusions: Conservative treatment of TB spondylitis of the lumbar spine can be treated with chemotherapy and appropriate investigations should be performed in patients with back pain who do not respond to rest, analgesics and physiotherapy. DOI: http://dx.doi.org/10.3126/noaj.v3i2.9526 NOAJ July-December 2013, Vol 3, Issue 2, 33-37
Fracture Dislocation of Shoulder
Authors: Manandhar Hemanta Kumar , Devkota Pramod , Khadka Padam Bahadur , Randey Chakra Raj 
Published: January 2014

Simultaneous dislocation of shoulder with fracture of the ipsilateral humerus is extremely rare and very little reported in the literature. We report a case of forty three year old man who got right shoulder dislocation with proximal humerus fracture. The patient was treated with open reduction and internal fixation by three 4.5mm screws. The operation went uneventful and has good range of motion of the shoulder after six months of follow up. DOI: http://dx.doi.org/10.3126/noaj.v3i2.9529 NOAJ July-December 2013, Vol 3, Issue 2, 44-45
'Not A Drop of Water...': State, Modernity and the Production of Nature in Spain, 1898-2010
Authors: Swyngedouw Erik 
Published: January 2014

Abstract
In this paper, I excavate the socio-environmental production of Spanish society during the twentieth century through the lens of the central role water politics, economics, culture and engineering played in Spain's tumultuous modernisation process. I explore first the origins of Spain's early-twentieth-century modernisation process (1890-1930) and the production of a national modernising imaginary as expressed in debates and actions around the hydrological condition. In the second part, I focus on how Spain's modernisation process after the Civil War became a profoundly scalar geographical project, articulated through the production of a specific technonatural hydraulic edifice during Franco's authoritarian rule (1939-1975). In the third part, I explore how the desalination of seawater has become one of the key contested terrains for managing hydro-scarcities since the transition to democracy. The broader intellectual objective of the paper is, first, to show how diverse political projects, social visions, physical matter, ecological concerns, cultural imaginaries, discursive formations, institutional practices, economic strategies of competitiveness and engineering technologies fuse together and articulate around specific hydro-technical imaginaries and infrastructures. Second, the paper documents how human and non-human actants become enrolled in such processes of hydro-social transformation. Third, the paper considers how the networks of actors sustaining hydro-social transformations mark the transition from a state-centred hydro-structural to a decentralised region-centred market environmentalist water framework.
The Aesthetics of the Volga and National Narratives in Russia
Authors: Zeisler-Vralsted Dorothy 
Published: January 2014

Abstract
Simon Schama's provocative work, Landscape and Memory , reveals the role that nature has historically played in shaping culture. Although much of Schama's focus predates the twentieth century, this essay on the historical development of the Volga River extends up to the present. The thesis, however, of demonstrating the continuity of earlier nature myths with the present, is an ongoing theme in this recounting of the effects of modernisation on the Volga while a nationalist rhetoric touts its aesthetic qualities. These aesthetics - symbolised through literary and artistic representations of Mother Volga - contributed to an emerging national narrative in Russia in the nineteenth century. This national identity is articulated through a rich visual culture that includes the work of Russian artists such as Isaac Levitan and Ilya Repin with their landscape portrayals of the Volga. As a result, the river assumed an iconic status which enhanced the story of its transformation. By the 1930s, the Volga underwent a major engineering project that rerouted part of the river into the Moscow Volga Canal. Construction of the project took four years, during which an array of journals, photographic essays and newspaper articles chronicled progress, celebrating this symbolic foray into the modern nation-state. Overlooking the beginning of the project were two towering statues of Lenin and Stalin, reinforcing the nationalist attributes of the project. In the 1930s Soviet Union, literature is rife with references to the conquest of nature in service of socialism. Harnessing rivers was especially attractive to Stalin in his push to modernise. But the Moscow Volga Canal was not just a showpiece - although in some instances the architecture rivaled the newly built metro stations - as it connected Moscow to the Caspian Sea, provided hydropower and supplied drinking water to Moscow. In conclusion, this essay will expand on nature's integral role in shaping culture with twentieth-century examples of how the Volga River continued to serve a nationalist discourse for a country in the throes of modernisation. The story of the Moscow Volga Canal is also a reminder that nature is often the repository to which nations look when crafting their identity.
Nature and Identity in the Construction of the Romanian Concept of Nation
Authors: Nicolescu Valentin Quintus 
Published: January 2014

Abstract
In this article I explore the role played by nature in the construction of the discourse of Romanian national identity. What was the perceived relation between the human community and the natural realm? How was the natural landscape transformed into a constitutive part of the national discourse? Could nature be seen as an essential identity marker for Romanian nationhood, thus shaping a form of Romanian exceptionalism? What form does this exceptionalist view take in the Romanian case? I highlight how the concept of an imagined national community was developed by the Romanian political and intellectual elite of the eighteenth and nineteenth centuries and the role played by nature in the shaping of this discourse of identity.
In vitro Cultivation of Hancornia speciosa Gomes : The Physical Constitution of the Culture Medium, Sucrose Concentrations and Growth Conditions
Authors: Cabral Juliana Silva Rodrigues , Alberto Paula Sperotto , Pereira Flávia Dionísio , Souchie Edson Luiz , Silva Fabiano Guimarães 
Published: January 2014

The effect of constitution of the culture medium, different concentrations of sucrose, and different growth conditions on the germination of seeds and growth of explants of "mangaba" (Hancornia speciosa Gomes) were evaluated in two in vitro assays. In assay one, the physical constitution of a medium (solid and liquid) and different concentrations of sucrose (0, 15, 30, 45, 60, 75, and 90 g/l) were tested, and seed germination was found to be variable. In assay two evaluation of the effect of different growth conditions (with and without agitation) on the in vitro growth of explants. Present results showed that "mangaba" had the highest percentage of germination and potentiality when inoculated in a liquid medium with (15 g/l) or without sucrose. The medium without agitation resulted in a better growth of explants the average length number of buds. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17519 Plant Tissue Cult. & Biotech. 23(2): 177-187, 2013 (December)
Callus Induction and In vitro Plantlet Regeneration of Gymnema sylvestre R. Br. (Retz.) and the Phytochemical Screening of Natural Plants and Callus Cultures
Authors: NAN Kaushalya , WTPSK Senarath 
Published: January 2014

Gymnema sylvestre is a slow growing perennial medicinal woody climber. It belongs to the family Asclepiadaceae. Gymnemic acid, the major bioactive component of this plant species is used as a remedy for type II diabetes. Propagation of this plant is often difficult and expensive. In the present study, in vitro protocols were developed in order to induce callus and regenerate plantlets from different explants of G. sylverstre. As secondary metabolites are important in medicinal plants, studies were carried out to screen phytochemicals present in natural plants and callus. The best medium for callus induction from leaf discs was MS supplemented with 5.0 mg/l 2,4-D. Although, nodal segment grown in MS supplemented with 1.0 mg/l BA gave the highest shoot elongation (14.8 ± 0.20), growth regulator free MS also showed a high elongation of shoots (14.2 ± 0.37) and the difference between those two were non-significant. MS supplemented with 3.0 mg/l IBA was best for root induction. Highest survival percentage (62.5) was observed when plantlets were acclimated in a substrate containing a mixture of soil and sand in the proportion of 1 : 2. In the present study, phytochemicals present in callus and the leaves of the naturally grown plants were compared using Gas Chromatography- Mass Spectrophotometer. A total of nine compounds was identified from the leaves of naturally grown plants and nine compounds were identified from the callus. Out of all identified phytochemicals, a total of six compounds were present in both leaves and callus samples suggesting that in addition the plant material, callus may also be used as a supplement raw material to obtain secondary metabolites for the pharmaceutical industry. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17521 Plant Tissue Cult. & Biotech. 23(2): 201-210, 2013 (December)
Micropropagation of Salacia reticulata - an Endangered Medicinal Plant
Authors: Dhanasri G. , Reddy M. Srikanth , Naresh B. , Cherku Devi 
Published: January 2014

An efficient protocol of axillary bud proliferation and direct organogenesis has been developed for Salacia reticulata, a highly important medicinal plant. Over-exploitation for its antidiabetic and antioxidant properties concentrated in roots and stem has caused it to be endangered, thereby the need for its conservation. Propagation of S. reticulata in vitro is a promising way for its conservation. To develop the micropropagation protocol, the germplasm was screened for selection of a suitable ecotype with high content of mangiferin estimated with High Performance Liquid Chromatography technique. Nodal segments were cultured on MS supplemented with different growth regulators. The most efficient shoot multiplication was obtained with the supplementation of BA and IAA (3.5 + 0.5 mg/l). Elongation of the micro-shoots was achieved by subculture every 20 days. The elongated micro-shoots were efficiently rooted in vitro on half strength MS supplemented with IBA. Plantlets were successfully established in the soil in 6 - 8 weeks and were morphologically similar to those of the source plant. The protocols developed presently for direct shoot regeneration and root-induction could be successfully applied for development of high quality planting stocks. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17523 Plant Tissue Cult. & Biotech. 23(2): 221-229, 2013 (December)
Cloning of the Plasma Membrane Sodium/Hydrogen Antiporter SOS1 for its Over expression in Rice
Authors: Razzaque Samsad , Elias Sabrina M. , Biswas Sudip , Haque Taslima , Seraj Zeba I. 
Published: January 2014

Soil salinity adversely affects plant growth, development and disturbs intracellular ion homeostasis which results in cellular toxicity. The salt overly sensitive 1 (SOS1) gene is a critical component of salt tolerance in many species and encodes a plasma membrane Na+/H+ antiporter that plays an important role in germination and growth in saline environments. In the current study, the coding sequence of SOS1 gene (3447 bp) was amplified from a Bangladeshi rice landrace, Pokkali, by applying the fusion PCR strategy. SOS1 PCR amplicons were firstly cloned into pENTR/D-TOPO and then recombined with the binary vector, pH7WG2, through LR reaction. Positive colonies were validated by PCR, restriction digestion and sequencing. Finally, the constructed vector was transformed into Agrobacterium tumeficiens, LBA4404 strain, to initiate Agrobacterium-mediated transformation with the provision to transfer the cloned SOS1 into farmer popular rice varieties. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17527 Plant Tissue Cult. & Biotech. 23(2): 263-273, 2013 (December)
The Phloroglucinol Conundrum: Increase in Root Growth of Hybrid Cymbidium (Orchidaceae) with no Toxic Effect on Protocorm-like Body Formation
Authors: Teixeira da Silva Jaime A. 
Published: January 2014

D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17528 Plant Tissue Cult. & Biotech. 23(2): 275-282, 2013 (December)
A long PCR–based approach for DNA enrichment prior to next‐generation sequencing for systematic studies
Authors: Uribe‐Convers Simon , Duke Justin R. , Moore Michael J. , Tank David C. 
Published: January 2014

• Premise of the study: We present an alternative approach for molecular systematic studies that combines long PCR and next‐generation sequencing. Our approach can be used to generate templates from any DNA source for next‐generation sequencing. Here we test our approach by amplifying complete chloroplast genomes, and we present a set of 58 potentially universal primers for angiosperms to do so. Additionally, this approach is likely to be particularly useful for nuclear and mitochondrial regions.• Methods and Results: Chloroplast genomes of 30 species across angiosperms were amplified to test our approach. Amplification success varied depending on whether PCR conditions were optimized for a given taxon. To further test our approach, some amplicons were sequenced on an Illumina HiSeq 2000.• Conclusions: Although here we tested this approach by sequencing plastomes, long PCR amplicons could be generated using DNA from any genome, expanding the possibilities of this approach for molecular systematic studies.
Peut-on comparer les grandes crises de 1873, 1929 et 2008 ?
Authors: Plihon Dominique 
Published: January 2014

L’histoire du capitalisme est jalonnée de crises récurrentes d’inégale gravité. Par sa profondeur, la crise, qui a débuté aux États-Unis en 2007 sur le marché des subprimes et s’est propagée à l’économie mondiale à partir de 2008, peut être comparée aux grandes crises de 1873 et 1929. Ces trois crises ont en commun une triple dimension financière, sociale et économique 1 .
Les « experts » de la PAC à la Confédération paysanne : saisir les logiques sociales de la représentation syndicale
Authors: Roullaud Élise 
Published: January 2014

Les travaux portant sur les logiques de la représentation partisane ou syndicale mettent en évidence une distinction sociale entre « mandataires » et « mandants » reposant notamment sur un capital scolaire et économique plus élevé pour les premiers. L’étude sociologique de la sélection des responsables nationaux de la Confédération paysanne et plus précisément de ceux considérés comme « experts » de la Politique agricole commune dévoile d’autres mécanismes distinctifs. Le présent article démontre ainsi non seulement le rôle déterminant de la constitution d’un capital militant spécifique mais également celui joué par les logiques organisationnelles dans la désignation des représentants syndicaux. En se focalisant sur la figure des « experts », cet article révèle en outre la relativité des ressources militantes dont la valeur dépend du contexte organisationnel dans lequel elles s’inscrivent.