bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-Octanoyl dopamine transiently inhibits T cell proliferation via G1 cell-cycle arrest and inhibition of redox-dependent transcription factors
Authors: Wedel Johannes , Hottenrott Maximillia C , Stamellou Eleni , Breedijk Annette , Tsagogiorgas Charalambos , Hillebrands Jan-Luuk , Yard Benito A 
Published: June 2014

AbstractRecently, we developed a nonhemodynamic dopamine derivative, NOD, which has profound anti-inflammatory effects in vitro. As NOD also protects rats from ischemic AKI, the present study tested whether NOD is able to modulate cellular immunity for potential use as a T cell-suppressive agent. To this end, T cells were stimulated by anti-CD3/CD28 or PMA/ionomycin in the presence or absence of different concentrations of NOD. T cell proliferation, activation markers, intracellular cytokine expression, and activation of transcription factors were assessed. Whereas T cell proliferation was inhibited significantly by NOD at Day 3, proliferation was restored at Day 7 or later depending on the NOD concentration used. Inhibition of proliferation was reflected by a diminished CD25 expression and switch from naive to memory T cells. Early TCR activation events were unaffected, yet NF-κB and AP-1 were strongly inhibited by NOD. The inhibitory effect of NOD seemed to be dependent on its redox activity, as NOT, a redox-inactive NOD derivate, did not influence proliferation. NOD displayed synergistic effects with CNIs on T cell proliferation. Our data demonstrate that NOD displays T cell-suppressive activity. In keeping with its anti-inflammatory action and its beneficial effect on ischemia-induced AKI, NOD may be an interesting drug candidate to prevent CNI-related side-effects.
Histone Deacetylase Inhibition Induces Long-Lasting Changes in Maternal Behavior and Gene Expression in Female Mice
Authors: Stolzenberg Danielle S. , Stevens Jacqueline S. , Rissman Emilie F. 
Published: June 2014

AbstractIn many species, including mice, maternal responsiveness is experience-dependent and permanent, lasting for long periods (months to years). We have shown that after brief exposures to pups, virgin female mice continue to respond maternally toward pups for at least one month. Administration of a histone deacetylase inhibitor (HDACi) reduces the amount of maternal experience required to affect maternal behavior and gene expression. In this set of studies, we examined the epigenetic mechanisms that underlie these motivated behaviors. We assessed whether the effects of HDACi persisted 1 month after the initial experience (in the absence of continued pup experience or HDACi treatment) and whether the maintenance of maternal memory was associated with stable changes in gene expression. Using chromatin immunoprecipitation, we examined whether Esr2 and Oxt gene expression might be mediated by recruitment of the histone acetyltransferase cAMP response element binding protein (CBP) to their promoter regions after maternal memory consolidation. We report that HDACi treatment induced long-lasting changes in maternal responsiveness. Maternal learning was associated with increased recruitment of CBP to the Esr2 and Oxt gene promoters during the consolidation of maternal memory as well as a persistent increase in estrogen receptor-β (Esr2) mRNA and decreased expression of the de novo DNA methyltransferase Dnmt3a within the medial preoptic area. The consolidation of the maternal experience may involve the CBP recruitment and stable changes in gene expression, which maintain increased maternal responsiveness for long periods of time.
Rab5 Activity Regulates GLUT4 Sorting Into Insulin-Responsive and Non-Insulin-Responsive Endosomal Compartments: A Potential Mechanism for Development of Insulin Resistance
Authors: Tessneer Kandice L. , Jackson Robert M. , Griesel Beth A. , Olson Ann Louise 
Published: June 2014

AbstractGlucose transporter isoform 4 (GLUT4) is the insulin-responsive glucose transporter mediating glucose uptake in adipose and skeletal muscle. Reduced GLUT4 translocation from intracellular storage compartments to the plasma membrane is a cause of peripheral insulin resistance. Using a chronic hyperinsulinemia (CHI)-induced cell model of insulin resistance and Rab5 mutant overexpression, we determined these manipulations altered endosomal sorting of GLUT4, thus contributing to the development of insulin resistance. We found that CHI induced insulin resistance in 3T3-L1 adipocytes by retaining GLUT4 in a Rab5-activity-dependent compartment that is unable to equilibrate with the cell surface in response to insulin. Furthermore, CHI-mediated retention of GLUT4 in this non-insulin-responsive compartment impaired filling of the transferrin receptor (TfR)-positive and TfR-negative insulin-responsive storage compartments. Our data suggest that hyperinsulinemia may inhibit GLUT4 by chronically maintaining GLUT4 in the Rab5 activity-dependent endosomal pathway and impairing formation of the TfR-negative and TfR-positive insulin-responsive GLUT4 pools. This model suggests that an early event in the development of insulin-resistant glucose transport in adipose tissue is to alter the intracellular localization of GLUT4 to a compartment that does not efficiently equilibrate with the cell surface when insulin levels are elevated for prolonged periods of time.
Role of the miR-17∼92 cluster family in cerebellar and medulloblastoma development
Authors: Zindy Frederique , Kawauchi Daisuke , Lee Youngsoo , Ayrault Olivier , Ben Merzoug Leila , McKinnon Peter J. , Ventura Andrea , Roussel Martine F. 
Published: June 2014

ABSTRACT
The miR-17∼92 cluster family is composed of three members encoding microRNAs that share seed sequences. To assess their role in cerebellar and medulloblastoma (MB) development, we deleted the miR-17∼92 cluster family in Nestin-positive neural progenitors and in mice heterozygous for the Sonic Hedgehog (SHH) receptor Patched 1 (Ptch1+/−). We show that mice in which we conditionally deleted the miR-17∼92 cluster (miR-17∼92floxed/floxed; Nestin-Cre+) alone or together with the complete loss of the miR-106b∼25 cluster (miR-106b∼25−/−) were born alive but with small brains and reduced cerebellar foliation. Remarkably, deletion of the miR-17∼92 cluster abolished the development of SHH-MB in Ptch1+/− mice. Using an orthotopic transplant approach, we showed that granule neuron precursors (GNPs) purified from the cerebella of postnatal day 7 (P7) Ptch1+/−; miR-106b∼25−/− mice and overexpressing Mycn induced MBs in the cortices of naïve recipient mice. In contrast, GNPs purified from the cerebella of P7 Ptch1+/−; miR-17∼92floxed/floxed; Nestin-Cre+ animals and overexpressing Mycn failed to induce tumors in recipient animals. Taken together, our findings demonstrate that the miR-17∼92 cluster is dispensable for cerebellar development, but required for SHH-MB development.
Photochemical Channel of Anisaldehyde Vapor Revealed by Phosphorescence Measurement
Authors: Itoh Takao 
Published: June 2014

Abstract
Change of the emission spectrum of anisaldehyde vapor has been investigated as a function of irradiation time by exposing a sample to UV light for different times. It is shown that there is a new photochemical channel for anisaldehyde (p-methoxybenzaldehyde) vapor to form benzaldehyde vapor upon the photoexcitation into the S2(π, π*) state.
Study of Aqueous Solutions of Surfactants in the Presence of Alcohols by Means of Ultrasonic Relaxation
Authors: Nishikawa Sadakatsu , Kawahara Takahiro , Tominaga Naotaka , Sugiyama Koutarou , Mizuta Koutarou 
Published: June 2014

Abstract
Ultrasonic absorption coefficients in aqueous solutions of surfactants, octyltrimethylammonium bromide, dodecyltrimethylammonium bromide, and tetradecyltrimethylammonium bromide have been measured in the presence of 2-propanol or ethanol in the frequency range from 0.8 to 220 MHz at 25 °C. The frequency dependence of the observed absorption in solution of octyltrimethylammonium bromide has been characterized by a Debye-type relaxation equation with one single relaxation frequency which decreases with the surfactant concentration. On the other hand, the dependences in solutions of dodecyltrimethylammonium bromide and cetyltrimethylammonium bromide have been well fitted to the equation with two relaxation frequencies. One relaxation frequency decreases with the surfactant concentrations, and the other relaxation frequency shows concentration independence trend. The relaxation mechanisms have been considered on the basis of interaction between surfactant monomer and alcohol aggregate and of a conformational change of the surfactants with long hydrophobic groups to which plural alcohol molecules (dumpling cake) attract. The rate and thermodynamic parameters have been determined for aqueous solutions of octyltrimethylammonium bromide coexisting with 2.00 mol dm−3 2-propanol and 3.00 mol dm−3 ethanol. It has been speculated from the analysis that the dumpling cake of alcohols attaches and detaches on and from the alkyl chains of the surfactants. The number of alcohols in the aggregate is dependent on size of alcohol molecule although the forward and backward rate constants are not very dependent on coexisting alcohols. However, only the orders of the rate constants for the systems of dodecyltrimethylammonium bromide solution with 2.00 mol dm−3 2-propanol have been speculated because of too many parameters which should be determined. Following the proposed reaction model, the relation between the surfactant concentration dependence of adiabatic compressibility and the solution characteristics has been discussed.
Project Managers' Practical Intelligence and Project Performance in Software Offshore Outsourcing: A Field Study
Authors: Langer Nishtha , Slaughter Sandra A. , Mukhopadhyay Tridas 
Published: June 2014

This study examines the role of project managers' (PM) practical intelligence (PI) in the performance of software offshore outsourcing projects. Based on the extant literature, we conceptualize PI for PMs as their capability to resolve project related work problems, given their long-range and short-range goals; PI is targeted at resolving unexpected and difficult situations, which often cannot be resolved using established processes and frameworks. We then draw on the information processing literature to argue that software offshore outsourcing projects are prone to severe information constraints that lead to unforeseen critical incidents that must be resolved adequately for the projects to succeed. We posit that PMs can use PI to effectively address and resolve such incidents, and therefore the level of PMs' PI positively affects project performance. We further theorize that project complexity and familiarity contribute to its information constraints and the likelihood of critical incidents in a project, thereby moderating the relationship between PMs' PI and project performance. To evaluate our hypotheses, we analyze longitudinal data collected in an in-depth field study of a leading software vendor organization in India. Our data include project and personnel level archival data on 530 projects completed by 209 PMs. We employ the critical incidents methodology to assess the PI of the PMs who led these projects. Our findings indicate that PMs' PI has a significant and positive impact on project performance. Further, projects with higher complexity or lower familiarity benefit even more from PMs' PI. Our study extends the literatures on project management and outsourcing by conceptualizing and measuring PMs' PI, by theorizing its relationship with project performance, and by positing how that relationship is moderated by project complexity and familiarity. Our study provides unique empirical evidence of the importance of PMs' PI in software offshore outsourcing projects. Given that PMs with high PI are scarce resources, our findings also have practical implications for the optimal resource allocation and training of PMs in software offshore services companies.
Rationing Capacity in Advance Selling to Signal Quality
Authors: Yu Man , Ahn Hyun-Soo , Kapuscinski Roman 
Published: June 2014

We consider a seller who can sell her product over two periods, advance and spot. The seller has private information about the product quality, which is unknown to customers in advance and publicly revealed in spot. The question we consider is whether the seller has an incentive to signal quality in advance and, if so, how she can convey a credible signal of product quality. We characterize the seller's signaling strategy and find that rationing of capacity in the advance period is an effective tool of signaling product quality. We find that the high-quality seller can distinguish herself by allocating less capacity than the low-quality seller in the advance period. We show that this signaling mechanism exists whenever advance selling would be optimal for both the high-quality and low-quality sellers if quality were known by the consumers. Interestingly, the seller's ability to ration (rationing flexibility) sometimes disadvantages the seller; this effect is independent of product quality. This paper was accepted by Yossi Aviv, operations management.
Managing anger at work: on the ward
Authors: Duckett Stephane 
Published: June 2014

While there is a significant amount of literature on anger management, there is relatively little specifically as it applies to ward based work. However, there are two seminal papers on this topic (Gans, 1983; McLaughlin and Carey 1993) that are extremely useful to the practicing health care professional. Gans outlines some of the key dynamics that can arise within ward based work, dynamics that can lead to reciprocal anger developing between patients' families and treatment teams. He outlines how families can find themselves caught between needing to grieve, and tremendous feelings of guilt that such emotions can cause in families who feel that grieving represents a betrayal or abandonment of hope. The feelings of vulnerability that can follow on such occasions can be overwhelming, and anger is often an easy way out.
Self-rated health among spinal cord injury survivors: directions for future research
Authors: Lysack Cathy , Neufeld Stewart , Machacova Katerina 
Published: June 2014

Aim: This study was conducted to further the understanding of self-rated health (SRH) in persons with spinal cord injury (SCI). Methods: A review of the literature identified four published studies and the National Spinal Cord Injury Statistical Center (NSCISC) database that have evaluated SRH in persons with SCI. This article discusses the SRH findings across the studies and the NSCISC database, and compares these to the broader SRH literature. Findings: People with SCI do not rate their health very differently from able-bodied persons and, for the most part, rate their health positively. SRH ratings for persons with SCI may be inextricably linked to age and secondary conditions. Conclusions: The analysis suggests that SRH as a concept has been underused in studies with people with SCI. Given the literature that points to the power of SRH to predict many important health outcomes, the authors urge greater consideration of the SRH measure in disability and rehabilitation research and suggest directions for conceptual and measurement development.
Methodological issues arising from a pilot RCT investigating the effectiveness of joint protection
Authors: Masterson Shirley , Bryer Melanie 
Published: June 2014

Aims: This article describes the difficulties encountered when attempting to evaluate the addition of joint protection education to an exercise programme in the treatment of patients with osteoarthritis (OA) of the hand. This involved a pilot study for a randomized controlled trial, which was carried out in order to inform the design of future studies. Methods: Subjects were recruited from rheumatology and orthopaedic out-patient clinics in a district general hospital for a 6 month period in 2006. They were assessed at baseline and 12 weeks after intervention. There were 2 intervention groups: one receiving exercise therapy and formal joint protection education from an occupational therapist over 2 sessions, supplemented with a joint protection information booklet, and the control group which received exercise therapy and informal joint protection advice. The primary outcome measures were pain and hand function measured using the hand pain visual analogue scale and the Cochin Hand Functional Disability Scale. Six patients were recruited with OA of the hand (mean age 59.2 years and mean disease duration 8.9 years) with three randomized to the intervention group, and three to the control group. Findings: There were methodological issues arising from the study, including sample recruitment issues, time scale, limited participant numbers and ethical constraints. No statistically valid data was gained to ascertain whether exercise therapy was more effective when given in conjunction with joint protection advice than without. Conclusions: Although some results were achieved that suggested there were improvements in the measures of perceived hand pain, health status, grip strength and pinch grip, these were not statistically meaningful. The improvements were greater in the control group, which received exercise therapy without joint protection advice. Hand function was reduced in both intervention groups. The reasons for this could not be answered in this study. A larger study would need to be undertaken in order to gain statistically valid data to evaluate the effectiveness of joint protection advice for people with OA hand.
Sexualidad y escuela. Perspectivas programáticas posibles
Authors: Wainerman Catalina , Chami Natalia 
Published: June 2014

La ley nacional 26.150 (2006) establece la obligación de todas las escuelas de Argentina de gestión estatal y privada de impartir un Programa Integral de Educación Sexual. Ya antes de la sanción de la ley, algunas escuelas habían puesto en práctica programas diversos de educación sexual. Para aprovechar estas experiencias espontáneas como insumos para el diseño de estrategias de implementación de la ley, se realizó una investigación en 24 escuelas de cuatro jurisdicciones. El objetivo central fue conocer las perspectivas programáticas y las estrategias pedagógicas utilizadas a partir de entrevistas con setenta y dos actores. El presente artículo expone algunos de los resultados alcanzados.
Differentiation State-Specific Mitochondrial Dynamic Regulatory Networks Are Revealed by Global Transcriptional Analysis of the Developing Chicken Lens
Authors: Chauss Daniel , Basu Subhasree , Rajakaruna Suren , Ma Zhiwei , Gau Victoria , Anastas Sara , Brennan Lisa A , Hejtmancik J Fielding , Menko A Sue , Kantorow Marc 
Published: June 2014

AbstractThe mature eye lens contains a surface layer of epithelial cells called the lens epithelium that requires a functional mitochondrial population to maintain the homeostasis and transparency of the entire lens. The lens epithelium overlies a core of terminally differentiated fiber cells that must degrade their mitochondria to achieve lens transparency. These distinct mitochondrial populations make the lens a useful model system to identify those genes that regulate the balance between mitochondrial homeostasis and elimination. Here we used an RNA sequencing and bioinformatics approach to identify the transcript levels of all genes expressed by distinct regions of the lens epithelium and maturing fiber cells of the embryonic Gallus gallus (chicken) lens. Our analysis detected more than 15,000 unique transcripts expressed by the embryonic chicken lens. Of these, more than 3000 transcripts exhibited significant differences in expression between lens epithelial cells and fiber cells. Multiple transcripts coding for separate mitochondrial homeostatic and degradation mechanisms were identified to exhibit preferred patterns of expression in lens epithelial cells that require mitochondria relative to lens fiber cells that require mitochondrial elimination. These included differences in the expression levels of metabolic (DUT, PDK1, SNPH), autophagy (ATG3, ATG4B, BECN1, FYCO1, WIPI1), and mitophagy (BNIP3L/NIX, BNIP3, PARK2, p62/SQSTM1) transcripts between lens epithelial cells and lens fiber cells. These data provide a comprehensive window into all genes transcribed by the lens and those mitochondrial regulatory and degradation pathways that function to maintain mitochondrial populations in the lens epithelium and to eliminate mitochondria in maturing lens fiber cells.
Testing Models of the APC Tumor Suppressor/β-Catenin Interaction Reshapes Our View of the Destruction Complex in Wnt Signaling
Authors: Yamulla Robert J , Kane Eric G , Moody Alexandra E , Politi Kristin A , Lock Nicole E , Foley Andrew V A , Roberts David M 
Published: June 2014

AbstractThe Wnt pathway is a conserved signal transduction pathway that contributes to normal development and adult homeostasis, but is also misregulated in human diseases such as cancer. The tumor suppressor adenomatous polyposis coli (APC) is an essential negative regulator of Wnt signaling inactivated in &gt;80% of colorectal cancers. APC participates in a multiprotein “destruction complex” that targets the proto-oncogene β-catenin for ubiquitin-mediated proteolysis; however, the mechanistic role of APC in the destruction complex remains unknown. Several models of APC function have recently been proposed, many of which have emphasized the importance of phosphorylation of high-affinity β-catenin-binding sites [20-amino-acid repeats (20Rs)] on APC. Here we test these models by generating a Drosophila APC2 mutant lacking all β-catenin-binding 20Rs and performing functional studies in human colon cancer cell lines and Drosophila embryos. Our results are inconsistent with current models, as we find that β-catenin binding to the 20Rs of APC is not required for destruction complex activity. In addition, we generate an APC2 mutant lacking all β-catenin-binding sites (including the 15Rs) and find that a direct β-catenin/APC interaction is also not essential for β-catenin destruction, although it increases destruction complex efficiency in certain developmental contexts. Overall, our findings support a model whereby β-catenin-binding sites on APC do not provide a critical mechanistic function per se, but rather dock β-catenin in the destruction complex to increase the efficiency of β-catenin destruction. Furthermore, in Drosophila embryos expressing some APC2 mutant transgenes we observe a separation of β-catenin destruction and Wg/Wnt signaling outputs and suggest that cytoplasmic retention of β-catenin likely accounts for this difference.
Correlation of Stress in Silicon Carbide Crystal and Frequency Shift in Micro-Raman Spectroscopy
Authors: Sugiyama N. , Yamada M. , Urakami Y. , Kobayashi M. , Masuda T. , Nishikawa K. , Hirose F. , Onda S. 
Published: June 2014

ABSTRACTThe correlation of stress in Silicon Carbide (SiC) crystal and frequency shift in micro- Raman spectroscopy was determined by an experimental method. We applied uniaxial stress to 4H- and 6H-SiC single crystal square bar specimen shaped with (0001) and (11-20) faces by four point bending test, under measuring the frequency shift in micro-Raman spectroscopy. The results revealed that the linearity coefficients between stress and Raman shift were -1.96 cm-1/GPa for FTO(2/4)E2 on 4H-SiC (0001) face, -2.08 cm-1/GPa for FTO(2/4)E2 on 4H-SiC (11-20) face and -2.70 cm-1/GPa for FTO(2/6)E2 on 6H-SiC (0001) face. Determination of these coefficients has made it possible to evaluate the residual stress in SiC crystal quantitatively by micro-Raman spectroscopy. We evaluated the residual stress in SiC substrate that was grown in our laboratory by utilizing the results obtained in this study. The result of estimation indicated that the SiC substrate with a diameter of 6 inch remained residual stress as low as ±15 MPa.