bluuebunny/crossref_metadata_2025_split huggingface dataset:
Considerations on the Aquaculture Development and on the Use of Veterinary Drugs: Special Issue for Fluoroquinolones—A Review
Authors: Quesada Silvia Pilco , Paschoal Jonas Augusto Rizzato , Reyes Felix Guillermo Reyes 
Published: August 2013

AbstractAquaculture has become an important source of fish available for human consumption. In order to achieve greater productivity, intensive fish cultivation systems are employed, which can cause greater susceptibility to diseases caused by viruses, bacteria, fungi, and parasites. Antimicrobial substances are compounds used in livestock production with the objectives of inhibiting the growth of microorganisms and treatment or prevention of diseases. It is well recognized that the issues of antimicrobial use in food animals are of global concern about its impact on food safety. This paper present an overview of the aquaculture production in the whole world, raising the particularities in Brazil, highlighting the importance of the use of veterinary drugs in this system of animal food production, and address the potential risks arising from their indiscriminate use and their impacts on aquaculture production as they affect human health and the environment. The manuscript also discusses the analytical methods commonly used in the determination of veterinary drug residues in fish, with special issue for fluroquinolones residues and with emphasis on employment of LC‐MS/MS analytical technique.
Identification of Sensory Attributes That Drive Consumer Liking of Commercial Orange Juice Products in Korea
Authors: Kim Mina K. , Lee Young‐Jin , Kwak Han Sub , Kang Myung‐woo 
Published: August 2013

AbstractOrange juice is a well‐accepted fruit juice, and its consumption increases steadily. Many studies have been conducted to understand the sensory characteristics of orange juice throughout its varying processing steps. Sensory language and consumer likings of food can be influenced by culture. The objective of this study is to evaluate the sensory characteristics of commercially available orange juices in Korea and identify drivers of liking for orange juices in Korea. A quantitative descriptive analysis was conducted using a trained panel (n = 10) to evaluate 7 orange juice samples in triplicates, followed by consumer acceptance tests (n = 103). Univariate and multivariate statistical analyses were conducted for data analysis. The sensory characteristics of commercially available orange juice were documented and grouped: group 1 samples were characterized by high in natural citrus flavors such as orange peel, orange flesh, citrus fruit, and grape fruit, whereas group 2 samples were characterized by processed orange‐like flavors such as over‐ripe, cooked‐orange, and yogurt. Regardless of orange flavor types, a high intensity of orange flavor in orange juice was identified as a driver of liking for orange juices in Korea. Three distinct clusters were segmented by varying sensory attributes that were evaluated by likes and dislikes. Overall, many similarities were noticed between Korean market segment and global orange juice market. By knowing the drivers of liking and understanding the distinct consumer clusters present in the Korean orange juice market, the orange juice industry could improve the strategic marketing of its products in Korea.
Influence of ketanserin on the effects of methylenedioxymethamphetamine on body temperature in the mouse
Authors: Docherty J. R. , Bexis S. 
Published: August 2013

Summary

We have investigated the ability of the 5HT2‐receptor antagonist ketanserin to affect the hyperthermia produced by methylenedioxymethamphetamine (MDMA) in conscious mice and examined whether α1‐adrenoceptor antagonist actions are involved.

Mice were implanted with intra‐abdominal temperature probes under anaesthesia and allowed 2 weeks recovery. MDMA (20 mg kg−1) was administered subcutaneously 30 min after vehicle or test antagonist and effects on body temperature monitored by telemetry.

Following vehicle, MDMA produced a slowly developing hyperthermia, reaching a maximum increase of 1.24 °C at 150 min postinjection. Ketanserin (0.5 mg kg−1) revealed a significant and marked early hypothermia to MDMA, an effect that is mimicked by the α1‐adrenoceptor antagonist prazosin (0.1 mg kg−1).

Functional studies revealed antagonist actions of ketanserin at α1‐adrenoceptors in rat aorta and rat vas deferens in vitro indicative of α1‐adrenoceptor antagonist actions at the concentration used in vivo.

In conclusion, ketanserin (0.5 mg kg−1) modulates the hyperthermic actions of MDMA in mice. Although we cannot rule out additional actions at 5HT2‐receptors, the actions of ketanserin are consistent with α1‐adrenoceptor antagonism. There is no clear evidence from this study that 5HT2‐receptors mediate the hyperthermic response to MDMA.
Targeted enrichment and high‐resolution digital profiling of mitochondrial DNA deletions in human brain
Authors: Taylor Sean D. , Ericson Nolan G. , Burton Joshua N. , Prolla Tomas A. , Silber John R. , Shendure Jay , Bielas Jason H. 
Published: August 2013

SummaryDue largely to the inability to accurately quantify and characterize de novo deletion events, the mechanisms underpinning the pathogenic expansion of mtDNA deletions in aging and neuromuscular disorders remain poorly understood. Here, we outline and validate a new tool termed ‘Digital Deletion Detection’ (3D) that allows for high‐resolution analysis of rare deletions occurring at frequencies as low as 1 × 10−8. 3D is a three‐step process that includes targeted enrichment for deletion‐bearing molecules, single‐molecule partitioning of genomes into thousands of droplets for direct quantification via droplet digital PCR, and breakpoint characterization using massively parallel sequencing. Using 3D, we interrogated over 8 billion mitochondrial genomes to analyze the age‐related dynamics of mtDNA deletions in human brain tissue. We demonstrate that the total deletion load increases with age, while the total number and diversity of unique deletions remain constant. Our data provide support for the hypothesis that expansion of pre‐existing mutations is the primary factor contributing to age‐related accumulation of mtDNA deletions.
Larval morphology of Neocamiarus kuscheli kuscheli Jeannel 1957 and Inocatops sp. (Coleoptera, Leiodidae, Camiarinae): the first modern descriptions of two members of the tribe Camiarini in comparison with other Camiarinae
Authors: Kilian Aleksandra , Ptak Maria 
Published: August 2013

AbstractThe first modern, detailed descriptions of mature larvae of the tribe Camiarini are presented. The larva of Neocamiarus kuscheli is redescribed, and the larva of an unidentified species of Inocatops is also described for the first time. Both are measured, illustrated and photographed. Morphological and chaetotaxic differences among mature larvae of both species are summarised. Inaccuracies of the previous description of the larva of N. kuscheli as well as unique features of each of the species are discussed. Larval characters of the described species are discussed with reference to other Camiarinae and Leiodidae and their gondwanian past.
Prognostic factors and outcomes for salvage surgery in patients with recurrent squamous cell carcinoma of the tongue
Authors: Goto Mitsuo , Hanai Nobuhiro , Ozawa Taijiro , Hirakawa Hitoshi , Suzuki Hidenori , Hyodo Ikuo , Kodaira Takeshi , Ogawa Tetsuya , Fujimoto Yasushi , Terada Akihiro , Kato Hisakazu , Hasegawa Yasuhisa 
Published: August 2013

AbstractAimRecurrence rates of oral cancer following primary treatment have been reported in the range of 25–48%. However, salvage therapy remains a critical challenge to improving outcomes. Here, we investigated prognostic factors and outcomes for salvage surgery in patients with recurrent oral tongue squamous cell carcinoma (OTSCC).MethodsWe retrospectively reviewed patients who were referred to Aichi Cancer Center, Japan, for the treatment of recurrent OTSCC. All patients included in the present study had undergone salvage surgery. Data to identify the predictive value of prognostic factors were available from 69 patients. Prognostic factors were assessed using Cox's proportional hazards regression analysis. Differences in overall survival between groups of patients were assessed by the log–rank test.ResultsIn all, 36 patients (52%) developed second recurrence or died, of which 21 (58%) occurred within 12 months of salvage surgery. Univariate analysis indicated that survival was significantly worse in patients with recurrent stage III or IV tumors, two or more positive cervical lymph nodes, levels IV or V positive cervical lymph nodes, extracapsular spread (ECS) of positive cervical lymph nodes and a disease‐free interval from initial treatment of less than 12 months. On multivariate analysis, ECS was an independent prognostic factor for overall survival after salvage surgery.ConclusionNeck status at the time of salvage surgery, particularly ECS is a significant prognostic factor for surgical salvage. Survival was also influenced by the stage of the recurrent tumor and disease‐free interval, suggesting that the biological features of recurrent tumors might impact on prognosis.
Association of plasma visfatin with risk of colorectal cancer: An observational study of Chinese patients
Authors: Chen Mingwei , Wang Youming , Li Yongxiang , Zhao Lili , Ye Shuai , Wang Shenyi , Yu Changjun , Xie Huijuan 
Published: August 2013

AbstractAimTo investigate the association between plasma visfatin levels and risk of early and advanced colorectal cancer (CRC).MethodsIn total, 358 CRC patients and 286 controls were enrolled. According to the T factor of the TNM system. cancer patients were divided into two subgroups: early and advanced cancer. Levels of visfatin, anthropometric and metabolic parameters, which were classified as low, medium, and high, based on the tertile distributions in the control group, were determined.ResultsThe visfatin levels in patients with advanced and early cancer were higher than in controls (least significant difference test, P = 0.004 and 0.013, respectively). The patients in the highest tertile of visfatin concentration presented significantly higher odds for early and advanced CRC, adjusted for potential confounding factors (odds ratio 3.37; 95% CI, 1.93–8.37; P = 0.011; odds ratio 2.38; 95% CI: 1.82–8.35; P = 0.015, respectively). The visfatin level correlated significantly with waist:hip ratio (P < 0.05 for all) among case and control participants. Plasma visfatin levels in early and advanced CRC yielded a receiver operating characteristic curve area of 72 and 86%, respectively. The optimal sensitivity and specificity were 73% and 57% in discriminating between early CRC and normal controls while they were 76% and 68% in discriminating between advanced CRC and normal controls.ConclusionAn increased level of visfatin was a strong risk factor for both early and advanced CRC in Chinese patients. Plasma visfatin levels might be a potential biomarker for CRC detection.
Poultry and fish intake and risk of esophageal cancer: A meta‐analysis of observational studies
Authors: Jiang Gengxi , Li Bailing , Liao Xiaohong , Zhong Chongjun 
Published: August 2013

AbstractAimMixed results regarding the association between white meat (including poultry and fish) intake and the risk of esophageal cancer (EC) have been reported. We performed a meta‐analysis to provide a quantitative assessment of this association.Method(s)Relevant studies were identified in MEDLINE until December 31, 2012. Summary relative risks (SRRs) with 95% confidence intervals (CIs) were pooled with a random‐effects model.ResultsA total of 20 articles, including 3990 cases with EC, were included in this meta‐analysis. Compared to individuals with the lowest level of fish intake, individuals with the highest fish intake were found to have reduced risk of EC (SRRs = 0.69; 95% CIs: 0.57–0.85), while poultry intake was not associated with EC (SRRs = 0.83; 95% CIs: 0.62–1.12). Total fish consumption is associated with reduced esophageal squamous cell carcinoma (ESCC) risk, while poultry consumption was not associated with ESCC risk. Additionally, neither poultry nor fish consumption was associated with esophageal adenocarcinoma risk.Conclusion(s)Our results suggest that fish consumption may have a potential role in EC prevention, while poultry intake has no effect. However, because the majority of data was from case‐control studies, further well‐designed prospective studies are warranted.
Regulatory dendritic cell expression of MHCII and IL‐10 are jointly requisite for induction of tolerance in a murine model of OVA‐asthma
Authors: Huang H. , Dawicki W. , Lu M. , Nayyar A. , Zhang X. , Gordon J. R. 
Published: August 2013

AbstractBackgroundAllergen‐presenting dendritic cells differentiated with IL‐10 (DC10) reverse the asthma phenotype in mice by converting their Th2 cells to regulatory T cells (Tregs). DC10 express elevated levels of IL‐10, but substantially reduced levels of MHCII and costimulatory molecules, so the relationships between these factors with each other and tolerogenicity have not been clearly elucidated.MethodsWe assessed the roles of these inputs in DC10 reversal of OVA‐associated asthma‐like disease by treating affected mice with OVA‐pulsed DC10 generated from wild‐type or IL‐10‐sufficient MHCII−/− or CD80/CD86−/− mice, or with MHCII‐intact IL‐10‐silenced DC10.ResultsIL‐10 silencing did not discernibly affect the cells' immunobiology (e.g., costimulatory molecules, chemokines), but it eliminated IL‐10 secretion and the cell's abilities to induce tolerance, as determined by assessments of airway hyper‐responsiveness, eosinophilia, and Th2 responses to recall OVA challenge. MHCII−/− DC10 expressed normal levels of IL‐10, but, nevertheless, were unable to induce allergen tolerance in asthma phenotype mice, while tolerance induced by CD80/CD86−/− DC10 was attenuated but not eliminated. We also assessed the induction of multiple Treg cell markers (e.g., ICOS, PD‐1, GITR) on pulmonary CD25+Foxp3+ cells in the treated mice. Wild‐type DC10 treatments upregulated expression of each marker, while neither IL‐10‐silenced nor MHCII−/− DC10 did so, and the CD80/86−/− DC10 induced an intermediate Treg cell activation phenotype.ConclusionBoth IL‐10 and MCHII expression by DC10 are requisite, but not sufficient for tolerance induction, suggesting that DC10 and Th2 effector T cells must be brought together in a cognate fashion in order for their IL‐10 to induce tolerance.
Effect of retinoic acid on gene expression profiles of bovine intramuscular preadipocytes during adipogenesis
Authors: Mizoguchi Yasushi , Moriya Makoto , Taniguchi Daiki , Hasegawa Akira 
Published: August 2013

AbstractTo investigate genes involved in intramuscular adipogenesis in ruminants, 16 genes with dramatic variable expression were selected. These were selected from the differentiation‐ and proliferation‐phase libraries of our previous serial analysis of gene expression (SAGE) studies of a clonal bovine intramuscular preadipocyte (BIP) cell line. We harvested the BIP cells over 12 days after adipogenic stimulation with all‐trans retinoic acid (ATRA). Quantitative real‐time PCR confirmed the earlier SAGE study results of the expression patterns of 15 of the genes. On day 6, TG accumulation increased significantly in the BIP cells but was completely inhibited in the 3T3‐L1 cells (the monogastric reference). ATRA enhanced expression levels of six genes whereas it suppressed expression of eight genes on day 3 of adipogenesis in the BIP cells. Forty‐eight hours after transfection, the messenger RNA expression level of the adipose differentiation‐related protein (ADFP), encoded by one of the upregulated genes, in the ADFP small interference RNA (siRNA)‐transfected cells was 3.5% of that in negative control‐transfected cells. Also, 6 days after induction the TG level in the ADFP siRNA‐transfected cells was 21.8% lower than that in negative control‐transfected cells. This analysis of gene expression profiles after ATRA treatment will contribute to our understanding of the molecular mechanisms involved in bovine intramuscular adipogenesis.
Normal Human Variation: Refocussing the Enhancement Debate
Authors: Kahane Guy , Savulescu Julian 
Published: August 2013

AbstractThis article draws attention to several common mistakes in thinking about biomedical enhancement, mistakes that are made even by some supporters of enhancement. We illustrate these mistakes by examining objections that John Harris has recently raised against the use of pharmacological interventions to directly modulate moral decision‐making. We then apply these lessons to other influential figures in the debate about enhancement. One upshot of our argument is that many considerations presented as powerful objections to enhancement are really strong considerations in favour of biomedical enhancement, just in a different direction. Another upshot is that it is unfortunate that much of the current debate focuses on interventions that will radically transform normal human capacities. Such interventions are unlikely to be available in the near future, and may not even be feasible. But our argument shows that the enhancement project can still have a radical impact on human life even if biomedical enhancement operated entirely within the normal human range.
A Decision‐Theory Approach to Interpretable Set Analysis for High‐Dimensional Data
Authors: Boca Simina M. , Bravo Héctor Corrada , Caffo Brian , Leek Jeffrey T. , Parmigiani Giovanni 
Published: August 2013

SummaryA key problem in high‐dimensional significance analysis is to find pre‐defined sets that show enrichment for a statistical signal of interest; the classic example is the enrichment of gene sets for differentially expressed genes. Here, we propose a new decision‐theory approach to the analysis of gene sets which focuses on estimating the fraction of non‐null variables in a set. We introduce the idea of “atoms,” non‐overlapping sets based on the original pre‐defined set annotations. Our approach focuses on finding the union of atoms that minimizes a weighted average of the number of false discoveries and missed discoveries. We introduce a new false discovery rate for sets, called the atomic false discovery rate (afdr), and prove that the optimal estimator in our decision‐theory framework is to threshold the afdr. These results provide a coherent and interpretable framework for the analysis of sets that addresses the key issues of overlapping annotations and difficulty in interpreting p values in both competitive and self‐contained tests. We illustrate our method and compare it to a popular existing method using simulated examples, as well as gene‐set and brain ROI data analyses.
Pre‐diagnosis cigarette smoking and overall survival in non‐Hodgkin lymphoma
Authors: Ollberding Nicholas J. , Evens Andrew M. , Aschebrook‐Kilfoy Briseis , Caces Donne Bennett D. , Weisenburger Dennis D. , Smith Sonali M. , Chiu Brian C.‐H. 
Published: August 2013

SummaryWe examined whether smoking prior to non‐Hodgkin lymphoma (NHL) diagnosis was associated with overall survival (OS) and conducted a meta‐analysis to assess the evidence relating pre‐diagnosis cigarette smoking with OS. Among 523 NHL patients, worse OS was suggested for greater pre‐diagnostic smoking habits when compared to never smokers. In the meta‐analysis (n = 5 patient populations), inferior OS was observed for greater number of cigarettes smoked per day, years of cigarette smoking, and pack‐years of cigarette smoking. The inferior survival was more pronounced for follicular than for diffuse large B cell lymphoma. Pre‐diagnosis cigarette smoking may adversely impact the survival of NHL patients.
National prospective study on the use of local haemostatic agents during partial nephrectomy
Authors: Lang Herve , Mouracade Pascal , Gimel Pierre , Bernhard Jean C. , Pignot Geraldine , Zini Laurent , Crepel Maxime , Rigaud Jerome , Salomon Laurent , Bellec Laurent , Vaessen Christophe , Roupret Morgan , Jung Jean L. , Mourey Eric , Martin Xavier , Bigot Pierre , Bruyère Franck , Berger Julien , Ansieau Jean P. , Salome Franck , Hubert Jacques , Pfister Christian , Trifard François , Gigante Marc , Baumert Herve , Méjean Arnaud , Patard Jean J. 
Published: August 2013

Objective
To assess the use of local haemostatic agents (HAs) in a prospective multicentre large series of partial nephrectomies (PNs).
Patients and Methods
Prospective National Observational Registry on the Practices of Haemostasis in Partial Nephrectomy (NEPHRON): the study was conducted in 54 French urological centres from 1 June to 31 December 2010.
In all, 570 consecutive patients undergoing a PN were enrolled in this study in a prospective manner. The data was collected prospectively via an electronic case‐report form: five different sheets were included for preoperative, perioperative, postoperative and follow‐up data respectively. Information related to haemostasis was analysed.
Results
The median patient age was 60 years and the mean (range) tumour size was 3.68 (0.19–15) cm.
An HA was primarily used in 71.4% of patients, with a statistically significant difference among surgical approaches (P = 0.024). In 91.8% of cases, a single use of a HA was sufficient for achieving haemostasis. The HA was used either alone (13.9%) or in association with sutures (80.3%).
One or more additional haemostatic action(s) was needed in 12.3% of the cases.
When comparing patients who received a HA with those who did not receive a HA, there was no statistical difference between the groups for tumour size (P = 0.542), collecting system drainage (P = 0.538), hospital stay (P = 0.508), operation time (P = 0.169), blood loss (P = 0.387) or transfusion rate (P = 0.713).
Conclusion
HAs are widely used by urologists during PN. Progress is needed for standardising HA application, especially for the timing of application. For the time being, the role of the HA in nephron‐sparing surgery is still to be evaluated.
Catheter Ablation of Atrial Flutter in Patients With Left Ventricular Assist Device Improves Symptoms of Right Heart Failure
Authors: Hottigoudar Rashmi U. , Deam Allen G. , Birks Emma J. , McCants Kelly C. , Slaughter Mark S. , Gopinathannair Rakesh 
Published: August 2013

Persistent atrial flutter (AFL) in left ventricular assist device (LVAD) recipients can result in loss of AV synchrony, impaired ventricular filling and right heart failure (RHF). The authors report the largest series of HeartMate II (HMII) patients who developed AFL with decompensated RHF, which successfully resolved with AFL ablation. Eight patients with HMII LVAD (mean age, 57±12 years) had medically refractory AFL, with 7 developing de novo AFL after LVAD implant (onset range, 2 days–22 months post‐implant). Three patients developed recurrent syncope, 2 had inappropriate implantable cardioverter‐defibrillator shocks, and 6 had new or escalating need for inotropes. All had features of decompensated RHF. Seven patients underwent electrophysiology testing where mapping confirmed typical counterclockwise AFL (mean AFL cycle length, 252±49 ms) and radiofrequency ablation of cavotricuspid isthmus restored sinus rhythm in all patients. Complete resolution of symptoms and signs of RHF with improved quality of life were noted in all. No procedural complications were noted. During a mean follow‐up of 9±5 months, all patients remained free of atrial flutter. Catheter ablation of AFL in LVAD patients is safe and highly effective, resulting in immediate and significant improvement in symptoms of RHF, and should be considered first‐line therapy for AFL in these patients.