bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistent Facial Ulcerations with a Unique Clinical Appearance: Possible Trigeminal Trophic Syndrome
Authors: Inoue Tadanori , Mihara Motoyuki , Nakayama Hidetoshi , Shimao Shuhei , Shimizu Yasuyuki 
Published: April 2015

AbstractWe reported a 53‐year‐old woman with a persistent facial ulcer which moved gradually from the right marginal portion of the nasal root to the right lateral canthus along the margin of the right lower eyelid. The ulcer healed spontaneously. A similar ulcer developed at the entrance of the right anterior naris. The clinical findings were suggestive of squamous cell carcinoma, deep mycosis, mycobacterial infection, or a related condition. However, no definitive diagnosis could be made from the clinicopathological findings or the laboratory examinations, including bacterial, mycobacterial, and fungal cultures. The characteristic location of the lesion, and course of the disease, however, suggested the diagnosis of trigeminal trophic syndrome.
Functional Analysis of HLA‐DR‐expressing Keratinocytes from Tuberculin Reactive Skin
Authors: Morikawa Mitsuya , Yasue Takashi , Ohashi Masaru 
Published: April 2015

AbstractWe examined the functional roles of HLA‐DR+ keratinocytes which were induced in vivo from tuberculin reactive skin. At 4 days after intradermal PPD injection, about 80% of keratinocytes obtained from the tuberculin reactive area expressed DR antigens. In 14 of 18 individuals examined, PPD‐pulsed DR+ keratinocyte fraction induced autologous T‐cell proliferation. The proliferative response was PPD‐dependent, antigen specific; it depended upon DR expression by the keratinocyte fraction, because it did not occur in the presence of the PPD‐nonpulsed DR+ keratinocyte fraction and was completely blocked by mouse monoclonal antibody to HLA‐DR. However, the antigen‐presenting capacity of the DR+ keratinocyte fraction appeared to be less than that of monocytes or the DR+ epidermal cell fraction. The DR+ keratinocyte fraction was also able to stimulate allogeneic T‐cell DNA synthesis, but the DR− keratinocyte fraction could not. The possible influences of contaminant Langerhans cells and mononuclear cells in the DR+ keratinocyte fraction were considered to be unlikely. These results suggest that DR+ keratinocytes, which are induced in vivo, can both present the antigen to autologous T cells and stimulate allogeneic T cells.
Cutaneous Metastasis of Renal Cell Carcinoma: An Electron Microscopic Study
Authors: Haruki Tomoe , Takahashi Shozo , Morohashi Masaaki , Maruyama Tomohiro , Ida Mitsuru 
Published: April 2015

AbstractA case of cutaneous metastasis of renal cell carcinoma in a 67‐year‐old man is described. Right side nephrectomy had been performed three years earlier, and a renal cell carcinoma was diagnosed. Following a bruise, the patient noticed an erythematous nodule on the scalp. Histological examination of this skin lesion showed atypical tumor cells consisting predominantly of clear cells with a honeycomb‐like or adenoid structure. Electron microscopic examination revealed that the tumor cells were divided into three groups: clear cells with abundant glycogen, dark cells with abundant mitochondria, and intermediate cells containing both glycogen and mitochondria in varying proportions. Some of the tumor cells with an adenoid pattern had a brush border‐like structure in the lumen. These results support the diagnosis of metastatic renal cell carcinoma. An electron microscopic approach may aid in diagnosis of cutaneous metastasis from renal cell carcinoma.
Stimulation of Fibronectin Secretion in Cultured Human Keratinocytes by Transforming Growth Factor‐β Not by Other Growth Inhibitory Substances
Authors: Hashiro Makoto , Matsumoto Kunio , Hashimoto Koji , Yoshikawa Kunihiko 
Published: April 2015

AbstractWe investigated the effects of various growth inhibitory substances on fibronectin secretion in cultured normal human keratinocytes. Fibronectin secretion in keratinocytes was expressed by two methods: immunofluorescent staining of cells with anti‐human fibronectin antibody, and sodium dodecyl‐sulphate‐polyacrylamide gel electrophoresis of [35S]‐labeled proteins secreted by the cells followed by autoradiography. With immunofluorescent staining, the extracellular fibronectin was observed as coarse fibrils only in some cells of the control culture, while cells treated with 20 ng/ml transforming growth factor‐βl (TGF‐β1) showed intense fluorescences in a radiating pattern around most of the cells, indicating that TGF‐β1 markedly increased fibronectin secretion in keratinocytes. Analysis of [35S]‐methionine labeled proteins also revealed that TGF‐β1 increased fibronectin secretion eight fold in culture medium. TGF‐β2 has also induced weak increase of fibronectin secretion 1.8 fold in keratinocytes.In contrast, other growth inhibitory substances, such as tumor necrosis factor‐α, 1,25‐dihydroxyvitamin D3 and cyclosporin A, did not show any significant effects on fibronectin secretion. These results indicated that increase of fibronectin secretion is not associated with growth inhibitory effect, but rather with a specific effect of TGF‐β.
Lowered Cu, Zn‐Superoxide Dismutase Activity in Human Malignant Skin Tumors
Authors: Hamanaka Hiroshi , Miyachi Yoshiki , Tachibana Takao , Imamura Sadao 
Published: April 2015

AbstractWe examined Cu, Zn‐superoxide dismutase (SOD) activities of fifteen malignant skin tumors (four malignant melanomas, three squamous cell carcinomas, four Bowen's diseases, two basal cell epitheliomas, one sebaceous epithelioma, and one extramammary Paget's disease) and compared them with those in adjacent normal tissues of the same patients. Though there were some individual differences, all the SOD activities in tumor tissues were significantly lower than those in adjacent normal tissues of the same subjects. The average SOD activity in tumor tissues was 12.01 ± 2.17 unit/mg protein, while that of normal tissues was 17.62 ± 2.85. These findings agree with previous reports from various other organ tumors.
An Unusual Mechanical Hyperkeratosis of the Soles
Authors: Yoshii Akira , Ono Tomomichi , Kayashima Ken‐ichi , Kitoh Masato 
Published: April 2015

AbstractA series of 13 patients with hyperkeratosis of both soles of the feet are presented. All patients developed hyperkeratosis after wearing the so‐called “health sandals”, which have multiple projections on their upper surfaces to stimulate the soles while walking. There was no evidence of hereditary or systemic factors producing the hyperkeratosis and it was concluded to be due to chronic mechanical stimulation of the soles.This side effect of the wearing of “health sandals” (health sandals keratosis) should be noted by dermatologists.
Purpuric Cutaneous Manifestations in Mitochondrial Encephalomyopathy
Authors: Horiguchi Yuji , Fujii Tatsuya , Imamura Sadao 
Published: April 2015

AbstractA six‐month‐old boy with mitochondrial myopathy, encephalopathy, lactic acidosis, and stroke‐like episodes (MELAS) syndrome developed repeated crops of purpuric macules on his soles and palms, which were histologically identified as hemorrhage into the dermis without inflammatory infiltrates. Transmission electron microscopy of the skin eruptions revealed various stages of endothelial degeneration in the dermal capillaries associated with consequent extravasation of erythrocytes. The degenerative change was characterized by swollen and vacuolated mitochondria which showed disintegration of their cristae. These morphological changes in the mitochondria of the endothelial cells resembled those seen in skeletal muscle fibers. Similar changes were also noted in other tissues of the skin, such as the axons of myelinated peripheral nerves and some of the keratinocytes in the epidermis. Although these fine structural features are difficult to differentiate from artifacts, abnormal mitochondria could result in functional disturbance particularly in the tissues that require relatively high kinetics, and thus contribute the symptoms of myopathy, encephalopathy, acidosis and stroke‐like episodes.
Cutaneous Porphyrias in China
Authors: Liao Kang‐Huang 
Published: April 2015

AbstractIn China, 78 cases of cutaneous porphyrias, together with 67 cases reported by other clinicians from 1979 to 1990, were reviewed. Of a total of 145 cases, 75.2% were EPP (109 cases), but only 28 cases (19.3%) were PCT. This prevalence differs from that in other parts of the world. The article discussed this disparity and some notable points. Early diagnosis of EPP using the fluorescence microscopic test for determination of RBC protoporphyrin is important. The complication of hepatobiliary aspects in PCT and EPP was noticed. Liver disease seems to be an important precipitating factor in China.
Anaplastic Large Cell Lymphoma, So‐called Ki‐1 Lymphoma: A Report of a Case of Long Course
Authors: Kanekura Takuro , Setoyama Mitsuru , Yotsumoto Shin‐ichi , Nishi Masayuki , Tashiro Masaaki 
Published: April 2015

AbstractA 55‐year‐old female with a complaint of tumors on the right lower extremity was reported. The condition was diagnosed as anaplastic large cell lymphoma, so‐called Ki‐1 lymphoma, by its histological and immunohistochemical features. The clonal proliferation of the infiltrating cells of the skin lesions was confirmed by the analysis of T cell receptor gene rearrangement. The lesions have repeatedly occurred on the right lower extremity for more than ten years.In this report, we also discuss the prognosis of anaplastic large cell lymphoma with or without skin lesions.
Tinea Corporis Due to Microsporum canis from an Asymptomatic Dog
Authors: Katoh Takuro , Maruyama Rhuji , Nishioka Kiyoshi , Sano Takao 
Published: April 2015

AbstractThe patient was a 19‐year‐old female student who purchased a puppy from a pet shop four weeks earlier. At the time of her first examination, an annular edematous erythema with adherent scales and vesicles surrounding its margin was seen on the left forearm. On direct examination of the vesicles, fungal elements were detected, and Microsporum canis was isolated. The puppy was a Pomeranian and was kept in the house at all times. No clinical lesions were seen on the puppy, and the Wood's lamp test was negative. However, M. canis was isolated from the animal by the hairbrush method. Symptoms disappeared after the patient was treated topically with terbinafine cream for three weeks. Although the dog received no treatment whatsoever, there was no evidence of the disease on the pet. Results of the hairbrush method performed on the pet two and three weeks later were negative, but, at five weeks, it was again positive. Human infection with M. canis from an asymptomatic dog was demonstrated in this case. Attention should be paid to preventing infections from animals without lesions.
Inhibition Spectra of Solar Urticaria: A Case Report and a Review of the Japanese Cases
Authors: Yamazaki Masashi , Kawada Akira , Noda Toshiaki , Shimizu Tatsuya , Hiruma Masataro , Ishibashi Akira 
Published: April 2015

AbstractA 34‐year‐old Japanese female with solar urticaria is described, who had an episode of urticarial response when she was exposed to indoor fluorescent light first and sunlight later. The action spectrum was found to range from 480 to 520 nm with an inhibition spectrum above 650 nm in the visible light by both postirradiation and preirradiation. Injection of in vitro‐irradiated serum did not produce an urticarial wheal on the patient. In the Japanese literature, 17 cases of solar urticaria with inhibition spectra have been reported. The wavelength of the inhibition spectrum was longer than that of the action spectrum in each case.
Histochemical Detection of Burn‐induced Lipid Peroxidation in Sebaceous Glands of Rat Skin
Authors: Kitajima Toshiyuki , Hamanaka Hiroshi , Miyachi Yoshiki , Ono Yoko , Moriguchi Takahiko , Toyokuni Shinya , Imamura Sadao 
Published: April 2015

AbstractIncreased lipid peroxide levels both in skin and serum have been reported after cutaneous thermal injury. However, it still remains unclear where lipid peroxides are produced at the site of burned skin. In the present study, a histochemical method using cold Schiff's reagent was applied in order to detect the localization of lipid peroxide.Schiff positivity was detected in sebaceous glands, and the extent of positivity seemed to correlate with the serum lipid peroxide levels. These results may suggest that lipid peroxides produced in sebaceous glands after thermal injury enter the blood stream and are partially responsible for the elevated serum lipid peroxide levels.
The ATPase‐staining of Langerhans Cells in Previously Stored Skin Tissues
Authors: Kim Young‐Keum , Miyauchi Hiroko , Horio Takeshi , Asada Yasuo 
Published: April 2015

AbstractThe ATPase‐staining of Langerhans cells is usually done on fresh, unfixed biopsy specimens. In the present study, we attempted to stain these cells in previously stored tissues of guinea pig skin. The ATPase activity disappeared after freezing or preservation in formalin or ethanol. In contrast, tissue specimens which had been stored in physiological saline at 4°C stained as well as fresh cells even after 14 days.
Dermatophytosis in Children and Adolescents: Epidemiological, Clinical, and Microbiological Aspects Changing with Age
Authors: Zienicke Helga C. , Korting Hans C. , Lukacs Andreas , Braun‐Falco Otto 
Published: April 2015

AbstractDuring a four year period, a total of 84 cases of dermatophytosis were seen in patients from 0 to 17 years of age attending our out‐patient department. The sex distribution was about equal in preadolescents. Males predominated among adolescents. A peak incidence at early school age was due to cases of tinea capitis, mainly caused by Microsporum canis. A peak among adolescents was due to cases of tinea pedis, mainly caused by Trichophyton rubrum. In general, patients with Trichophyton rubrum infections were older than those with other types of dermatophytosis (p<0.05). Dermatophytosis due to Microsporum canis was most frequently seen in January and in September (p<0.01). Although onychomycosis due to dermatophytes was a comparatively rare disease, it did occur within all age subgroups. Entities which were very frequent in former times and which have become rare during the last decades, such as tinea capitis due to Trichophyton schönleinii, still contribute to the spectrum of dermatophytosis in childhood.
Lichen Nitidus: A Histologic and Electron Microscopic Study
Authors: Mihara Motoyuki , Nakayama Hidetoshi , Shimao Shuhei 
Published: April 2015

AbstractA lesion of lichen nitidus in a 53‐year‐old man was examined by histology and electron microscopy. The histology showed typical features. The electron microscopy revealed primary changes in hydropic degeneration of basal cells which possibly indicated severe edema between the basal keratinocytes and in the subepidermal area. The basal keratinocytes themselves were not particularly altered, except for clustered micro‐vacuolar structures in a small portion of the peripheral cytoplasm. In one of the eight blocks observed, the cytoplasmic process of a mast cell was found between the basal keratinocytes, indicating that mast cells may possibly infiltrate the epidermis in lichen nitidus. Scattered mast cells in the dermis were also noted. Thus primary pathologic changes of lichen nitidus may be induced by severe edema, which was accompanied by inflammatory cells including mast cells, in the region of the dermal‐epidermal junction.
Monoclonal Antibody OKB19, Reactive with a B‐Lymphoid Differentiation Antigen (CD19), Binding to Basal Layer Keratinocytes of Normal Human Skin
Authors: Watanabe Shinichi , Ichikawa Eiko , Wagatsuma Keiko , Kawa Yohko , Takahashi Hisashi 
Published: April 2015

AbstractMonoclonal antibody (moAB) OKB19 reacts with CD19 antigen, which is the broadest lineage‐specific surface marker on B‐lymphocytes. In frozen tissue sections, using an immunohistochemical technique, the OKB19‐positive cells in the basal layer were sharply demarcated from the negative suprabasal layers. In normal hair follicles, the OKB19 reactivity was also confined to one layer of the dermal side of the outer root sheath. However, this reactivity gradually disappeared in the lower areas. The inner surface of the lumina in the eccrine duct was weakly stained with OKB19. The basal keratinocytes were also stained with OKB19 in the lesional epidermis of the various dermatoses examined in this study, when the basal keratinocytes remained unaffected. Even in the hyperproliferative state of psoriasis, the OKB19 reactivity was confined to the basal layer. Several kinds of tumor cells derived from the skin were not stained with OKB19. No labeling was seen even in the basaloid cells of basal cell carcinoma, which are morphologically similar to basal keratinocytes. B4 and Leu‐12, other monoclonal antibodies reacting with CD19, did not recognize any keratinocytes in the normal human skin. MoAB OKB19, therefore, reacts with an antigen present on basal keratinocytes and provides a probe for the isolation of the basal keratinocyte subpopulation. Thus, this antibody should be useful in studies of not only B‐lymphocyte differentiation, but also normal and aberrant differentiation of the epidermal keratinocytes.
Lymphomatoid Papulosis: Clinicopathological Comparative Study with Pityriasis Lichenoides et Varioliformis Acuta
Authors: Erpaiboon Prayoon , Mihara Ichiro , Niimura Michihito 
Published: April 2015

AbstractWe have compared the clinical and histopathological features of 6 patients with lymphomatoid papulosis (LP) and 14 patients with pityriasis lichenoides et varioliformis acuta (PLEVA). There were some differences between the clinical features in the two diseases, including the size and appearance of skin lesions and the duration of the course of disease. Ki‐1 Ag positive, large, atypical, lymphoid cells were always seen in lymphomatoid papulosis; none of lymphoid cells of pityriasis lichenoides et varioliformis acuta demonstrated this antigen. We conclude that lymphomatoid papulosis and PLEVA, although sharing some common features, should be considered to be different clinical and immunopathological entities.
Immunohistochemical Differential Diagnosis between Lymphocytoma Cutis and Malignant Lymphoma in Paraffin‐embedded Sections
Authors: Toyota Noriaki , Matsuo Shinobu , Iizuka Hajime 
Published: April 2015

AbstractA panel of monoclonal antibodies (Leucocyte Common Antigen [CD45], LN‐1 [CDw75], LN‐2 [CD74], LN‐3, L‐26, UCHL‐1 [CD45RO] and MT‐1) which identify B and T cell surface markers were applied to benign lymphocytoma cutis and malignant lymphoma of the skin in paraffin‐embedded sections.In five cases of lymphocytoma cutis, both B and T cell markers were positive, consistent with the polyclonality of the proliferative lymphocytes. In contrast, in two cases of B cell lymphoma, only the B cell markers were positive, consistent with the monoclonality.Furthermore, the immunohistochemistry clarified lymph follicle structures of lymphocytoma cutis. These were occasionally not evident in routine hematoxylin and eosin stained sections.Thus an immunologic phenotyping study combined with other appropriate criteria for histologic evaluation and clonal gene rearrangement analysis appears to be useful in distinguishing reactive from neoplastic lymphoid lesions of the skin.
A Case Report of EMO Syndrome Showing Localized Hyperhidrosis in Pretibial Myxedema
Authors: Kato Naoko , Ueno Hiroo , Matsubara Miyao 
Published: April 2015

AbstractWe describe here a case of EMO syndrome, which is defined as a combination of exophthalmos, pretibial or localized myxedema, and hypertrophic osteoarthropathy. A 34‐year‐old Japanese man with Graves' disease developed the characteristic eye changes. He showed 22 mm protrusion of both eyes and hypertrophy of the right lateral rectus muscle as well as both superior rectus muscles by computerized tomography. He subsequently developed lightly erythematous, indurated, nonpitting, peau d'orange plaques and nodules on his lower legs. Finally, he developed Graves' acropathy with hypertrophic osteoarthropathy in the metacarpal bones. Histological examination of myxedematous skin showed typical deposition of mucin accompanied by changes in the amount and distribution of elastic fibers. In addition, the lesional skin showed localized hyperhidrosis, a rarely reported complication of pretibial myxedema. We speculate that this hyperhidrosis of the lesional skin was brought about by stimulation of peripheral sympathetic nerves by surrounding mucin deposition, in the setting of poorly controlled hyperthyroidism.
Leiomyosarcoma of the Skin with Generalized Metastases: Electron Microscopic and Immunohistochemical Study
Authors: Kinoshita Shinsuke , Hirai Akio , Izaki Seiichi , Kitamura Keijiro , Itoyama Shinji , Mukai Makio , Okamura Rieko 
Published: April 2015

AbstractA 62‐year‐old woman with numerous (more than 50) tumors between 25 and 75 mm in diameter disseminated over most of her body surface is reported. Biopsied tissue specimens demonstrated that the tumors consisted mainly of spindle‐shaped cells with nuclear pleomorphisms and relatively high mitotic rates. They were irregularly arranged; they sometimes mimicked storiform‐ and palisading‐patterns.Immunohistochemistry revealed that the tumor cells were positive for muscle‐specific actin, desmin, and myosin, but negative for S‐100 protein. Electron microscopy confirmed myofilaments and dense patches in the cytoplasm. The metastatic potential of such leiomyosarcoma of the skin is indicated.
Efficacy of Bimolane in the Malassezia ovalis Model of Psoriasis
Authors: Xu Bin , Noah Patricia W. , Skinner Robert B. , Bale George , Chesney Thomas McC. , Rosenberg E. William 
Published: April 2015

AbstractBimolane, an analog of razoxane has been used in China with comparable efficacy but less toxicity than razoxane in the treatment of psoriasis. In an attempt to characterize further its mode of action it was administered both systemically and topically in the Malassezia ovalis animal model of psoriasis.Intravenous methotrexate and topical 0.1% betamethasone valerate were also used as positive control treatments.The animal model of psoriasis was effectively treated by bimolane, both systemically and topically, and also by parenteral methotrexate and topical betamethosone valerate.The time course of bimolane's effect with this model was different from methotrexate's suggesting the possibility of a different mode of action.Because bimolane, like razoxane, is an ethylene diamino tetraacetate acid (EDTA) derivative, it is possible that its effects on this reaction relate to its chelating properties and that inhibition of complement activation is important to its mode of action.