bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asparagine attenuates intestinal injury, improves energy status and inhibits AMP-activated protein kinase signalling pathways in weaned piglets challenged withEscherichia colilipopolysaccharide
Authors: Wang Xiuying , Liu Yulan , Li Shuang , Pi Dingan , Zhu Huiling , Hou Yongqing , Shi Haifeng , Leng Weibo 
Published: August 2015

The intestine requires a high amount of energy to maintain its health and function; thus, energy deficits in intestinal mucosa may lead to intestinal damage. Asparagine (Asn) is a precursor for many other amino acids such as aspartate, glutamine and glutamate, which can be used to supply energy to enterocytes. In the present study, we hypothesise that dietary supplementation of Asn could alleviate bacterial lipopolysaccharide (LPS)-induced intestinal injury via improvement of intestinal energy status. A total of twenty-four weaned piglets were assigned to one of four treatments: (1) non-challenged control; (2) LPS+0 % Asn; (3) LPS+0·5 % Asn; (4) LPS+1·0 % Asn. On day 19, piglets were injected with LPS or saline. At 24 h post-injection, piglets were slaughtered and intestinal samples were collected. Asn supplementation improved intestinal morphology, indicated by higher villus height and villus height:crypt depth ratio, and lower crypt depth. Asn supplementation also increased the ratios of RNA:DNA and protein:DNA as well as disaccharidase activities in intestinal mucosa. In addition, Asn supplementation attenuated bacterial LPS-induced intestinal energy deficits, indicated by increased ATP and adenylate energy charge levels, and decreased AMP:ATP ratio. Moreover, Asn administration increased the activities of key enzymes involved in the tricarboxylic acid cycle, including citrate synthase, isocitrate dehydrogenase and α-ketoglutarate dehydrogenase complex. Finally, Asn administration decreased the mRNA abundance of intestinal AMP-activated protein kinase-α1 (AMPKα1),AMPKα2, silent information regulator 1 (SIRT1) and PPARγ coactivator-1α (PGC1α), and reduced intestinal AMPKα phosphorylation. Collectively, these results indicate that Asn supplementation alleviates bacterial LPS-induced intestinal injury by modulating the AMPK signalling pathway and improving energy status.
Acute consumption of a caffeinated energy drink enhances aspects of performance in sprint swimmers
Authors: Lara Beatriz , Ruiz-Vicente Diana , Areces Francisco , Abián-Vicén Javier , Salinero Juan José , Gonzalez-Millán Cristina , Gallo-Salazar César , Del Coso Juan 
Published: August 2015

AbstractThis study investigated the effect of a caffeinated energy drink on various aspects of performance in sprint swimmers. In a randomised and counterbalanced order, fourteen male sprint swimmers performed two acute experimental trials after the ingestion of a caffeinated energy drink (3 mg/kg) or after the ingestion of the same energy drink without caffeine (0 mg/kg; placebo). After 60 min of ingestion of the beverages, the swimmers performed a countermovement jump, a maximal handgrip test, a 50 m simulated competition and a 45 s swim at maximal intensity in a swim ergometer. A blood sample was withdrawn 1 min after the completion of the ergometer test. In comparison with the placebo drink, the intake of the caffeinated energy drink increased the height in the countermovement jump (49·4 (sd 5·3) v. 50·9 (sd 5·2) cm, respectively; P<0·05) and maximal force during the handgrip test with the right hand (481 (sd 49) v. 498 (sd 43) N; P<0·05). Furthermore, the caffeinated energy drink reduced the time needed to complete the 50 m simulated swimming competition (27·8 (sd 3·4) v. 27·5 (sd 3·2) s; P<0·05), and it increased peak power (273 (sd 55) v. 303 (sd 49) W; P<0·05) and blood lactate concentration (11·0 (sd 2·0) v. 11·7 (sd 2·1) mm; P<0·05) during the ergometer test. The caffeinated energy drink did not modify the prevalence of insomnia (7 v. 7 %), muscle pain (36 v. 36 %) or headache (0 v. 7 %) during the hours following its ingestion (P>0·05). A caffeinated energy drink increased some aspects of swimming performance in competitive sprinters, whereas the side effects derived from the intake of this beverage were marginal at this dosage.
Ageing, retirement and changes in vegetable consumption in France: findings from the prospective GAZEL cohort
Authors: Plessz Marie , Guéguen Alice , Goldberg Marcel , Czernichow Sébastien , Zins Marie 
Published: August 2015

AbstractThe aim of this study was to describe the change in vegetable consumption with ageing and the transition to retirement. Study subjects were the participants of the GAZEL prospective cohort (Gaz and Électricité de France) aged 40–49 years at inclusion in 1989 who retired between 1991 and 2008 (12 942 men and 2739 women). Four FFQ were completed from 1990 to 2009. We used multiple imputation by chained equations in order to avoid dropping incomplete cases. The OR for eating vegetables everyday was estimated as a function of ageing, retirement status and the place of lunch before retirement through generalised estimating equations. Analyses were stratified by sex, and models were adjusted for confounders, including current spousal status. In 1990, 17·7 % of men and 31 % of women reported eating vegetables daily. The odds of consuming vegetables everyday increased with ageing for both men and women. The usual place of lunch was home for less than half the sample before retirement and for almost every respondent after retirement. For those who changed their place of lunch, the association between being retired and the odds of eating vegetables daily was positive and significant. We found that, in this cohort, vegetable consumption increased with ageing. Retirement had an indirect effect on vegetable consumption mediated by changes in the place of lunch.
Morphology of the oxyurid nematodesTrypanoxyuris (T.) cacajaon. sp. andT. (T.) ucayaliin. sp. from the red uakari monkeyCacajao calvus ucayaliiin the Peruvian Amazon
Authors: Conga D.F. , Giese E.G. , Serra-Freire N.M. , Bowler M. , Mayor P. 
Published: August 2015

AbstractCacajao calvus ucayalii(Thomas, 1928) (Primates: Pitheciidae), a subspecies endemic to the Peruvian Amazon, occurs in patchy and sometimes isolated populations in north-eastern Peru and is in a vulnerable situation, mainly due to habitat loss and hunting. This rareness and remote distribution means that, until now, parasitical studies have been limited. Based on optical and scanning electron microscopy of specimens of both sexes, we report two new species ofTrypanoxyurispinworms occurring in the large intestine of the Peruvian red uakari, namelyTrypanoxyuris (Trypanoxyuris) cacajaoandTrypanoxyuris (Trypanoxyuris) ucayalii. Both species showed a distinct morphology of the lips and cephalic structure. Sexual dimorphism in the lateral alae was observed in both male and the female worms, with ventral ornamentation being shown in the oesophageal teeth. The finding of these new pinworm species highlights the possibility of discovering other species.
Intracellular expression profile and clinical significance of the CCR9–CCL25 chemokine receptor complex in nasopharyngeal carcinoma
Authors: Ye L-F , Huang J , Zhang L-P , Chen Z 
Published: August 2015

AbstractObjectives:This study aimed to investigate the association of C-C chemokine receptor type 9 (CCR9) and C-C motif chemokine 25 (CCL25) expression levels with clinical and tumour–node–metastasis stage in nasopharyngeal carcinoma.Methods:A total of 42 nasopharyngeal carcinoma patients (nasopharyngeal carcinoma group) and 18 patients with a normal nasopharynx (control group) were included in this study. Tissues were collected during surgery and medical examinations. The CCR9 and CCL25 messenger RNA and protein levels were measured using quantitative reverse transcription polymerase chain reaction, Western blotting and immunohistochemical analysis.Results:CCR9 and CCL25 messenger RNA and protein levels were significantly increased in the nasopharyngeal carcinoma group compared with the control group (p< 0.05). Both CCR9 and CCL25 messenger RNA and protein levels were significantly higher in advanced-stage nasopharyngeal carcinoma (stages III and IV) patients compared with early-stage nasopharyngeal carcinoma (stages I and II) patients (p< 0.05).Conclusion:The extent of CCR9 and CCL25 upregulation in nasopharyngeal carcinoma correlates with the tumour–node–metastasis stage.
Sleeping on the floor decreases insecticide treated bed net use and increases risk of malaria in children under 5 years of age in Mbita District, Kenya
Authors: MINAKAWA NOBORU , KONGERE JAMES O. , DIDA GABRIEL O. , IKEDA ERIKO , HU JINPING , MINAGAWA KOGOMI , FUTAMI KYOKO , KAWADA HITOSHI , NJENGA SAMMY M. , LARSON PETER S. 
Published: August 2015

SUMMARYChildren who sleep on the floor are less likely to use long-lasting insecticidal nets (LLINs); however, the relationship between sleeping location and Plasmodium falciparum infection has not been investigated sufficiently. This study revealed whether sleeping location (bed vs floor) is associated with P. falciparum infection among children 7–59 months old. More than 60% of children slept on the floor. Younger children were significantly more likely to sleep in beds [odds ratio, OR 2·31 (95% confidence interval (CI) 2·02–2·67)]. Nearly 70% of children slept under LLINs the previous night. LLIN use among children who slept on the floor was significantly less than ones sleeping in beds [OR 0·49 (95% CI 0·35–0·68)]. The polymerase chain reaction (PCR) based P. falciparum infection rate and slide based infection rate were 65·2 and 29·7%, respectively. Both infections were significantly higher among children slept on the floor [OR1·51 (95% CI 1·08–2·10) for PCR base, OR 1·62 (95% CI 1·14–2·30) for slide base] while net availability was not significant. Sleeping location was also significant for slide based infection with fever (⩾37·5 °C) [2·03 (95% CI 1·14–3·84)] and high parasitemia cases (parasite ⩾2500 µL−1) [2·07 (95% CI 1·03–4·50)]. The results suggest that sleeping location has a direct bearing on the effectiveness of LLINs.
Models for managing wildlife disease
Authors: McCALLUM HAMISH 
Published: August 2015

SUMMARYModelling wildlife disease poses some unique challenges. Wildlife disease systems are data poor in comparison with human or livestock disease systems, and the impact of disease on population size is often the key question of interest. This review concentrates specifically on the application of dynamic models to evaluate and guide management strategies. Models have proved useful particularly in two areas. They have been widely used to evaluate vaccination strategies, both for protecting endangered species and for preventing spillover from wildlife to humans or livestock. They have also been extensively used to evaluate culling strategies, again both for diseases in species of conservation interest and to prevent spillover. In addition, models are important to evaluate the potential of parasites and pathogens as biological control agents. The review concludes by identifying some key research gaps, which are further development of models of macroparasites, deciding on appropriate levels of complexity, modelling genetic management and connecting models to data.
Integrating immune mechanisms to model nematode worm burden: an example in sheep
Authors: GARNIER ROMAIN , GRENFELL BRYAN T. , NISBET ALASDAIR J. , MATTHEWS JACQUELINE B. , GRAHAM ANDREA L. 
Published: August 2015

SUMMARYGastrointestinal nematodes represent important sources of economic losses in farmed ruminants, and the increasing frequency of anthelmintic resistance requires an increased ability to explore alternative strategies. Theoretical approaches at the crossroads of immunology and epidemiology are valuable tools in that context. In the case of Teladorsagia circumcincta in sheep, the immunological mechanisms important for resistance are increasingly well-characterized. However, despite the existence of a wide range of theoretical models, there is no framework integrating the characteristic features of this immune response into a tractable phenomenological model. Here, we propose to bridge that gap by developing a flexible modelling framework that allows for variability in nematode larval intake which can be used to track the variations in worm burdens. We parameterize this model using data from trickle infection of sheep and show that using simple immunological assumptions, our model can capture the dynamics of both adult worm burdens and nematode fecal egg counts. In addition, our analysis reveals interesting dose-dependent effects on the immune response. Finally, we discuss potential developments of this model and highlight how an improved cross-talk between empiricists and theoreticians would facilitate important advances in the study of infectious diseases.
A life history intervention for individuals with dementia: a randomised controlled trial examining nursing staff empathy, perceived patient personhood and aggressive behaviours
Authors: ERITZ HEATHER , HADJISTAVROPOULOS THOMAS , WILLIAMS JAIME , KROEKER KRISTINE , R. MARTIN RONALD , M. LIX LISA , V. HUNTER PAULETTE 
Published: August 2015

ABSTRACTBehaviours of concern (e.g.aggression) are often present in residents of long-term care (LTC) facilities diagnosed with dementia and may impact quality of life. Prior uncontrolled research has shown that an intervention involving sharing resident life histories may be effective in reducing aggressive behaviours and improving quality of life, perhaps by increasing staff empathy. We used a randomised controlled design, involving a considerably larger sample than previous investigations. We also examined staff perceptions of LTC resident personhood in relation to aggressive behaviour. Seventy-three residents were randomised to either a life history intervention (N = 38) or a control condition (N = 35). Ninety-nine nurses and care aides answered questionnaires about their own attitudes and the residents' behaviours and quality of life at baseline, post-intervention and at follow-up. Results of mixed-effects modelling indicated significant differences between groups in personhood perception and resident quality of life. Personhood perception mediated the relationship between the intervention and improved quality of life. We identified significant negative correlations between resident cognitive impairment and staff perceptions of resident personhood. Qualitative findings suggested that staff primarily changed their verbal interactions with residents following the intervention, which may be particularly helpful for residents with the most severe dementia. Our results indicate that LTC residents benefit when life histories are constructed with their families and shared with nursing staff.
Design, analysis, and testing of a motor-driven capsule robot based on a sliding clamper
Authors: Gao Jinyang , Yan Guozheng , He Su , Xu Fei , Wang Zhiwu 
Published: August 2015

SUMMARYWe propose a motor-driven capsule robot based on a sliding clamper (MCRSC), a device to explore the partially collapsed and winding intestinal tract. The MCRSC is powered by wireless power transmission based on near-field inductive coupling. It comprises a novel locomotion unit, a camera, and a three-dimensional receiving coil, all installed at both ends of the locomotion unit. The novel locomotion unit comprises a linear motion mechanism and a sliding clamper. The former adopts a pair of lead-screw and nut to obtain linear motion, whereas the latter anchors the MCRSC to a specific point of the intestinal tract by expanding its arc-shaped legs. The MCRSC is capable of two-way locomotion, which is activated by alternately executing linear motion and anchoring action. Ex vivo experiments have shown that the MCRSC is able to inspect the colon within a time frame of standard colonoscopy.
A low-cost hardware-in-the-loop-simulation testbed of quadrotor UAV and implementation of nonlinear control schemes
Authors: Xian Bin , Zhao Bo , Zhang Yao , Zhang Xu 
Published: August 2015

SUMMARYDesigning and testing flight control algorithms for quadrotor UAVs (unmanned aerial vehicles) is not an easy task due to the risk of possible danger and damage during the practical flight. In order to improve the safety and efficiency of the flight control implementation, a low-cost real-time HILS (hardware-in-the-loop simulation) testbed for quadrotor UAVs is developed in this paper. To realize the HILS testbed, a miniature quadrotor is used as the main body, equipped with a micro AHRS (attitude heading reference system) unit and a self-build DSP (digital signal processor) board. The HILS is implemented by using xPC target. A compact PC/104 computer is utilized as the target computer, and a laptop PC is employed as the host computer. A desktop PC is used as flight visualization computer which runs FlightGear and Google Earth to show visual data, such as orientation and flight path of the quadrotor UAV. This testbed can be utilized for simulating various flight control algorithms, without losing safeness and reliableness. To demonstrate the effectiveness of the proposed testbed, a new nonlinear adaptive sliding mode based stabilization control algorithm is developed and verified on the HILS testbed.
Orthostatic hypotension and right heart failure as the initial manifestation of intravenous leiomyomatosis
Authors: Li Ya-Qin , Yin Xiao-Ping , Xu Zhan-Wen 
Published: August 2015

AbstractA 36-year-old woman, who had a history of myomectomy, presented with lightheadedness after changing position from sitting to standing and effort-related shortness of breath. Echocardiography demonstrated a hyperechoic elongated mobile mass extending from the inferior caval vein to the right atrium. The mass was surgically removed, and histological examination established the diagnosis of intravenous leiomyomatosis. This case caught the attention of our cardiology group to consider the diagnosis when an inferior caval vein or right atrium mass is found in a patient with a history of uterine leiomyomatosis.
The Green Eating Project: web-based intervention to promote environmentally conscious eating behaviours in US university students
Authors: Monroe Jessica T , Lofgren Ingrid E , Sartini Becky L , Greene Geoffrey W 
Published: August 2015

AbstractObjectiveTo investigate the effectiveness of an online, interactive intervention, referred to as the Green Eating (GE) Project, to motivate university students to adopt GE behaviours.DesignThe study was quasi-experimental and integrated into courses for credit/extra credit. Courses were randomly stratified into experimental or non-treatment control. The 5-week intervention consisted of four modules based on different GE topics. Participants completed the GE survey at baseline (experimental,n241; control,n367) and post (experimental,n187; control,n304). The GE survey has been previously validated and consists of Transtheoretical Model constructs including stage of change (SOC), decisional balance (DB: Pros and Cons) and self-efficacy (SE: School and Home) as well as behaviours for GE. Modules contained basic information regarding each topic and knowledge items to assess content learning.SettingThe GE Project took place at a public university in the north-eastern USA.SubjectsParticipants were full-time students between the ages of 18 and 24 years.ResultsThe GE Project was effective in significantly increasing GE behaviours, DB Pros, SE School and knowledge in experimental compared with control, but did not reduce DB Cons or increase SE Home. Experimental participants were also more likely to be in later SOC for GE at post testing.ConclusionsThe GE Project was effective in increasing GE behaviours in university students. Motivating consumers towards adopting GE could assist in potentially mitigating negative consequences of the food system on the environment. Future research could tailor the intervention to participant SOC to further increase the effects or design the modules for other participants.
Targeting the γ-/β-secretase interaction reduces β-amyloid generation and ameliorates Alzheimer’s disease-related pathogenesis
Authors: Cui Jin , Wang Xiaoyin , Li Xiaohang , Wang Xin , Zhang Chenlu , Li Wei , Zhang Yangming , Gu Haifeng , Xie Xin , Nan Fajun , Zhao Jian , Pei Gang 
Published: August 2015

AbstractDespite decades of intense global effort, no disease-modifying drugs for Alzheimer’s disease have emerged. Molecules targeting catalytic activities of γ-secretase or β-site APP-cleaving enzyme 1 (BACE1) have been beset by undesired side effects. We hypothesized that blocking the interaction between BACE1 and γ-secretase subunit presenilin-1 (PS1) might offer an alternative strategy to selectively suppress Aβ generation. Through high-throughput screening, we discovered that 3-α-akebonoic acid (3AA) interferes with PS1/BACE1 interaction and reduces Aβ production. Structural analogs of 3AA were systematically synthesized and the functional analog XYT472B was identified. Photo-activated crosslinking and biochemical competition assays showed that 3AA and XYT472B bind to PS1, interfere with PS1/BACE1 interaction, and reduce Aβ production, whereas sparing secretase activities. Furthermore, treatment of APP/PS1 mice with XYT472B alleviated cognitive dysfunction and Aβ-related pathology. Together, our results indicate that chemical interference of PS1/BACE1 interaction is a promising strategy for Alzheimer’s disease therapeutics.
Insights in the ecology and evolutionary history of the Miscellaneous Crenarchaeotic Group lineage
Authors: Fillol Mireia , Auguet Jean-Christophe , Casamayor Emilio O , Borrego Carles M 
Published: August 2015

Abstract
Members of the archaeal Miscellaneous Crenarchaeotic Group (MCG) are among the most successful microorganisms on the planet. During its evolutionary diversification, this very diverse group has managed to cross the saline–freshwater boundary, one of the most important evolutionary barriers structuring microbial communities. However, the current understanding on the ecological significance of MCG in freshwater habitats is scarce and the evolutionary relationships between freshwater and saline MCG remains poorly known. Here, we carried out molecular phylogenies using publicly available 16S rRNA gene sequences from various geographic locations to investigate the distribution of MCG in freshwater and saline sediments and to evaluate the implications of saline–freshwater transitions during the diversification events. Our approach provided a robust ecological framework in which MCG archaea appeared as a core generalist group in the sediment realm. However, the analysis of the complex intragroup phylogeny of the 21 subgroups currently forming the MCG lineage revealed that distinct evolutionary MCG subgroups have arisen in marine and freshwater sediments suggesting the occurrence of adaptive evolution specific to each habitat. The ancestral state reconstruction analysis indicated that this segregation was mainly due to the occurrence of a few saline–freshwater transition events during the MCG diversification. In addition, a network analysis showed that both saline and freshwater MCG recurrently co-occur with archaea of the class Thermoplasmata in sediment ecosystems, suggesting a potentially relevant trophic connection between the two clades.
Erratum: The diversity and host interactions of Propionibacterium acnes bacteriophages on human skin
Authors: Liu Jared , Yan Riceley , Zhong Qiao , Ngo Sam , Bangayan Nathanael J , Nguyen Lin , Lui Timothy , Liu Minghsun , Erfe Marie C , Craft Noah , Tomida Shuta , Li Huiying 
Published: August 2015

Abstract
Correction to: The ISME Journal (2015) 9, 2092–2107; doi:10.1038/ismej.2015.47; published online 7 April 2015 Since the publication of this article, the authors have noticed errors in Figures 4a and 6, where one Propionibacterium acnes strain, HL103PA1, was mislabeled in Figure 4a, and the CRISPR spacers were shown incorrectly in Figure 6.