bluuebunny/crossref_metadata_2025_split huggingface dataset:
Projet pédagogique et ingénierie de l’unité didactique
Authors: Puren Christian 
Published: August 2013

La nouvelle « perspective actionnelle » ébauchée dans le Cadre Européen Commun de Référence pour les Langues (CECRL, Didier 2001) se donne comme finalité la formation d’un « acteur social ». Cette finalité amène tout naturellement à considérer le « projet pédagogique » comme activité de référence parce qu’il engage par définition les apprenants comme des acteurs sociaux à part entière de leur propre projet d’apprentissage dans l’espace de la classe et pendant le temps de leur apprentissage. C’est (ou ce devrait être) particulièrement le cas dans des environnements d’enseignement-apprentissage tels que les IUT, où les projets pédagogiques proposés par les enseignants peuvent soit simuler de futures activités professionnelles, soit correspondre à des projets réels tels que ceux impliquant la conception, la préparation, la réalisation et l’exploitation de stages de terrain à l’étranger. On se propose d’examiner ici la nouvelle question d’ingénierie didactique qui se pose du coup aux enseignants et concepteurs de matériels didactiques : comment concilier la logique (d’autonomie maximale) et le temps (long) des projets pédagogiques avec un dispositif ‒ ladite « unité didactique » ‒ qui reste malgré tout nécessaire, mais qui obéit à une logique contraire (celle de la pré-programmation des contenus et des activités) et à un temps plus court.
Sonographic features of agenesis of dorsal pancreas
Authors: Vijayaraghavan S Boopathy , Gouru Swapna , Senthil Sathiya 
Published: August 2013

AbstractAgenesis of dorsal pancreas is an extremely rare congenital anomaly that occurs due to failure of the dorsal pancreatic bud to form the body and tail of the pancreas. We report the sonographic appearance of this condition in six cases.
Giant sacrococcygeal teratoma embolization
Authors: Rossi Umberto G , Cariati Maurizio , Tomà Paolo 
Published: August 2013

AbstractResection of giant sacrococcygeal teratoma with high-vasculature in newborns can be a fatal procedure due to massive bleeding of the tumor. Endovascular embolization of the arteries that supply the tumor may lead to minimal blood loss. We present a case of giant high-vascular sacrococcygeal teratoma type-1 that was embolized in an infant born at 35 weeks gestation. This procedure lead to a safe, surgical resection with minimal bleeding: 12 ml.
An Efficient Hybrid DSMC/MD Algorithm for Accurate Modeling of Micro Gas Flows
Authors: Liang Tengfei , Ye Wenjing 
Published: August 2013

AbstractAiming at simulating micro gas flows with accurate boundary conditions, an efficient hybrid algorithm is developed by combining the molecular dynamics (MD) method with the direct simulation Monte Carlo (DSMC) method. The efficiency comes from the fact that the MD method is applied only within the gas-wall interaction layer, characterized by the cut-off distance of the gas-solid interaction potential, to resolve accurately the gas-wall interaction process, while the DSMC method is employed in the remaining portion of the flow field to efficiently simulate rarefied gas transport outside the gas-wall interaction layer. A unique feature about the present scheme is that the coupling between the two methods is realized by matching the molecular velocity distribution function at the DSMC/MD interface, hence there is no need for one-to-one mapping between a MD gas molecule and a DSMC simulation particle. Further improvement in efficiency is achieved by taking advantage of gas rarefaction inside the gas-wall interaction layer and by employing the “smart-wall model” proposed by Barisiket al.The developed hybrid algorithm is validated on two classical benchmarks namely 1-D Fourier thermal problem and Couette shear flow problem. Both the accuracy and efficiency of the hybrid algorithm are discussed. As an application, the hybrid algorithm is employed to simulate thermal transpiration coefficient in the free-molecule regime for a system with atomically smooth surface. Result is utilized to validate the coefficients calculated from the pure DSMC simulation with Maxwell and Cercignani-Lampis gas-wall interaction models.
Androgen activity and markers of inflammation among men in NHANES III
Authors: Schooling C. Mary 
Published: August 2013

ABSTRACTObjectivesInflammation contributes to chronic diseases. Lower serum testosterone among men is associated with less inflammation, yet immune defense is thought to trade‐off against reproduction with androgens adversely affecting immune function. Anti‐androgens are effective at castrate levels of serum testosterone, suggesting serum testosterone may not capture all androgen activity. The association of two androgen biomarkers with key markers of inflammation was examined.MethodsThe adjusted association of serum testosterone and androstanediol glucuronide with C‐reactive protein, white blood cell, granulocyte and lymphocyte count, fibrinogen, and hemoglobin, as a control outcome because testosterone administration raises hemoglobin, were examined in a nationally representative sample of 1,490 US men from the National Health and Nutrition Examination Survey III phase 1 (1988–1991) using multivariable linear regression.ResultsSerum testosterone and androstanediol glucuronide were weakly correlated (0.13). Serum testosterone was associated with lower white blood cell count [−0.26 × 10−9 per standard deviation, 95% confidence interval (CI) −0.37 to −0.14] and granulocyte count (−0.21 × 10−9, 95% CI −0.29 to −0.13) but not with hemoglobin (0.02 g/l, 95% CI −0.89 to 0.92), adjusted for age, education, race/ethnicity, smoking, and alcohol. Similarly adjusted, androstanediol glucuronide was not associated with white blood cell count (0.10 × 10−9, 95% CI −0.05 to −0.25), granulocyte count (0.12 × 10−9, 95% CI −0.02 to 0.25), or fibrinogen (0.05 g/l, 95% CI −0.004 to 0.11), but was with hemoglobin (0.70 g/l, 95% CI 0.07 to 1.32).ConclusionsDifferent androgen biomarkers had different associations with inflammatory markers, highlighting the need to consider several androgen biomarkers. The possibility remains that androgens may generate inflammatory processes with implications for chronic diseases. Am. J. Hum. Biol., 25:622–628, 2013. © 2013 Wiley Periodicals, Inc.
Achondroplasia with multiple‐suture craniosynostosis: A report of a new case of this rare association
Authors: Bessenyei Beáta , Nagy Andrea , Balogh Erzsébet , Novák László , Bognár László , Knegt Alida C. , Oláh Éva 
Published: August 2013

AbstractWe report on a female patient with an exceedingly rare combination of achondroplasia and multiple‐suture craniosynostosis. Besides the specific features of achondroplasia, synostosis of the metopic, coronal, lambdoid, and squamosal sutures was found. Series of neurosurgical interventions were carried out, principally for acrocephaly and posterior plagiocephaly. The most common achondroplasia mutation, a p.Gly380Arg in the fibroblast growth factor receptor 3 (FGFR3) gene, was detected. Cytogenetic and array CGH analyses, as well as molecular genetic testing of FGFR1, 2, 3 and TWIST1 genes failed to identify any additional genetic alteration. It is suggested that this unusual phenotype is a result of variable expressivity of the common achondroplasia mutation. © 2013 Wiley Periodicals, Inc.
De novo 13q deletions in two patients with mild anorectal malformations as part of VATER/VACTERL and VATER/VACTERL‐like association and analysis of EFNB2 in patients with anorectal malformations
Authors: Dworschak Gabriel C. , Draaken Markus , Marcelis Carlo , de Blaauw Ivo , Pfundt Rolph , van Rooij Iris A.L.M. , Bartels Enrika , Hilger Alina , Jenetzky Ekkehart , Schmiedeke Eberhard , Grasshoff‐Derr Sabine , Schmidt Dominik , Märzheuser Stefanie , Hosie Stuart , Weih Sandra , Holland‐Cunz Stefan , Palta Markus , Leonhardt Johannes , Schäfer Mattias , Kujath Christina , Rißmann Anke , Nöthen Markus M. , Zwink Nadine , Ludwig Michael , Reutter Heiko 
Published: August 2013

AbstractAnorectal malformations (ARMs) comprise a broad spectrum of conditions ranging from mild anal anomalies to complex cloacal malformations. In 40–50% of cases, ARM occurs within the context of defined genetic syndromes or complex multiple congenital anomalies, such as VATER/VACTERL (vertebral defects [V], ARMs [A], cardiac defects [C], tracheoesophageal fistula with or without esophageal atresia [TE], renal malformations [R], and limb defects [L]) association. Here, we report the identification of deletions at chromosome 13q using single nucleotide polymorphism‐based array analysis in two patients with mild ARM as part of VATER/VACTERL and VATER/VACTERL‐like associations. Both deletions overlap the previously defined critical region for ARM. Heterozygous Efnb2 murine knockout models presenting with mild ARM suggest EFNB2 as an excellent candidate gene in this region. Our patients showed a mild ARM phenotype, closely resembling that of the mouse. We performed a comprehensive mutation analysis of the EFNB2 gene in 331 patients with isolated ARM, or ARM as part of VATER/VACTERL or VATER/VACTERL‐like associations. However, we did not identify any disease‐causing mutations. Given the convincing argument for EFNB2 as a candidate gene for ARM, analyses of larger samples and screening of functionally relevant non‐coding regions of EFNB2 are warranted. In conclusion, our report underlines the association of chromosome 13q deletions with ARM, suggesting that routine molecular diagnostic workup should include the search for these deletions. Despite the negative results of our mutation screening, we still consider EFNB2 an excellent candidate gene for contributing to the development of ARM in humans. © 2013 Wiley Periodicals, Inc.
Neuromotor synapses in Escobar syndrome
Authors: Robinson Karyn G. , Viereck Matthew J. , Margiotta Megan V. , Gripp Karen W. , Abdul‐Rahman Omar A. , Akins Robert E. 
Published: August 2013

AbstractThe Escobar variant of multiple pterygium syndrome (OMIM #265000) is a rare, autosomal recessive disorder associated with mutations in the γ‐subunit of the nicotinic acetylcholine receptor (CHRNG). CHRNG is expressed in fetal muscle during motor development and contributes to the formation of neuromuscular junctions (NMJs). Anomalies in NMJ structure and function have not been investigated in patients with Escobar syndrome. We report five patients identified as having Escobar syndrome, from four families. In three families, the same mutation (c.459dupA) was identified in CHRNG. A biopsy from brachioradialis muscle was collected from a patient from one of these families and analyzed for NMJ organization using fluorescence microscopy. Compared to spinalis muscle from control patients with idiopathic scoliosis or cerebral palsy (CP), the patient with Escobar syndrome had a significantly higher degree of acetylcholine receptor present outside acetylcholinesterase and significantly less acetylcholinesterase outside acetylcholine receptors. Given the role of the acetylcholine receptor γ‐subunit in fetal neuromuscular signal transduction and in establishing the primary encounter of muscle and motor nerve terminal, the CHRNG mutations described in Escobar syndrome may cause a broader disruption of postsynaptic proteins and result in aberrant development of the NMJ due to impaired prenatal neuromuscular transmission and/or abnormal neuromuscular synaptogenesis. © 2013 Wiley Periodicals, Inc.
Compatibilizing effect of halloysite nanotubes in polar–nonpolar hybrid system
Authors: Pal Parthajit , Kundu Mrinal Kanti , Malas Asish , Das Chapal Kumar 
Published: August 2013

ABSTRACTThis article explores the effect of halloysite nanotubes (HNTs) and modified HNTs (M‐HNTs) on the properties of immiscible blend system based on polar polyoxymethylene (POM) and nonpolar polypropylene (PP) polymers. HNTs have been modified by N‐(β‐aminoethyl)‐γ‐aminopropyltrimethoxysilane (APTMS). Modification is confirmed by Fourier transform infrared spectroscopy (FTIR), also FTIR confirms the interaction between polymer blend and HNTs/M‐HNTs. Morphology of the nanocomposites are demonstrated by scanning electron microscope (SEM) and dispersion of HNTs/M‐HNTs are observed by transmission electron microscope (TEM). In nanocomposites, average dispersed domain sizes reduce in the presence of HNTs/M‐HNTs but significant reduction has been observed in the case of M‐HNT‐filled nanocomposites rather than unmodified HNT‐filled nanocomposites. The M‐HNT acts as a reinforcing agent as well as bridging tool in polar–nonpolar hybrid system. Modification of HNTs brings compatibility in between the blend partners and reveals improved dynamic mechanical, thermal, and tensile properties than that of the pure blend system. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39587.
A success story: water primroses, aquatic plant pests
Authors: Thouvenot Lise , Haury Jacques , Thiebaut Gabrielle 
Published: August 2013

ABSTRACTAquatic ecosystems are currently invaded by non‐indigenous aquatic plants. A major challenge for biological invasion research is to develop the ability to predict the spread of species.Throughout the world,Ludwigia peploidessubsp.montevidensisandLudwigia grandiflorasubsp.hexapetalaare now considered to be two of the most invasive aquatic plants. This paper reviews the scientific knowledge about these species, their ecological and socio‐economic impacts and methods of management.Water primrose possesses some advantageous biological traits, such as rapid growth rate, efficient reproductive capacity, high plasticity in growth response, broad ecological tolerance and allelopathic compounds which might explain its expansion and colonization of numerous ecosystems.Much attention must be paid toLudwigia grandiflorasubsp.hexapetalaandLudwigia peploidessubsp.montevidensis. According to climatic models, global warming will further increase the spread of these species in Europe.Copyright © 2013 John Wiley & Sons, Ltd.
Ex vivo cytokine production in peripheral blood mononuclear cells after their stimulation with dsRNA of natural origin
Authors: Veinalde Rūta , Petrovska Ramona , Brūvere Rūta , Feldmane Guna , Pjanova Dace 
Published: August 2013

AbstractDouble‐stranded RNA (dsRNA) is a pathogen‐associated molecular pattern, known for its ability to induce antiviral response and enhance communication between cells mediating innate and adaptive immune responses. The aim of this study was to characterize the effect of the dsRNA‐containing product Larifan on the production of a wide spectrum of cytokines and chemokines in ex vivo cultivated peripheral blood mononuclear cells. Concentrations of 29 different cytokines were detected by a Luminex® 200™ System using three Milliplex MAP Multiplex Assay Kits. Larifan caused strong induction of chemokine macrophage inflammatory protein 1, I‐309, and TARC, proinflammatory cytokines IL‐6, tumor necrosis factor ‐, granulocyte macrophage colony‐stimulating factor, anti‐inflammatory IL‐10, and cellular immunity mediating factors IL‐23 and interferon‐. Considerable suppression of IL‐16 and chemokine stromal cell‐derived factor 1 a+b and interferon gamma‐induced protein 10 was also observed. The network of molecules responding to the presence of Larifan revealed the pleiotropic effect this product exerts on immune response.
Expression of codon optimized human bone morphogenetic protein 4 in Pichia pastoris
Authors: Huang Yide , Zhen Binqiong , Lin Yao , Cai Yanhui , Lin Zhen , Deng Chunmei , Zhang Yanding 
Published: August 2013

AbstractBone morphogenetic proteins (BMPs) are TGF‐β family member proteins that have therapeutic potential. The amount of BMPs from natural resources is limited, and the production of biologically active BMPs in heterologous protein expression systems remains an obstacle for their clinical application. In this study, the DNA sequence of human BMP4 mature domain (hBMP4) was optimized according to the codon relative synonymous codon usage values in Pichia pastoris, and the A+T content in the sequence after optimization was within the range of 30% to 55%. In Pichia pastoris cultured in shake‐flask, the expression level of hBMP4 protein from the optimized sequence (48 mg/L) increased fourfold in comparison with that from the native sequence (12 mg/L). Recombinant hBMP4 protein was purified by SP Sepharose and heparin affinity chromatography. The biological activities of recombinant hBMP4 were examined by measuring proliferation stimulation in cells and induction of ectopic cartilage formation in mouse models. Our results demonstrated that the optimized DNA sequence could significantly enhance hBMP4 protein expression in Pichia pastoris compared with the native sequence and produce biologically active recombinant hBMP4; this indicates the potential of this optimized sequence for bulk production of hBMP4 protein in future clinical applications.
Enzymatic hydrolysis of pelletized AFEX™‐treated corn stover at high solid loadings
Authors: Bals Bryan D. , Gunawan Christa , Moore Janette , Teymouri Farzaneh , Dale Bruce E. 
Published: August 2013

ABSTRACTAmmonia fiber expansion (AFEX™) pretreatment can be performed at small depots, and the pretreated biomass can then be pelletized and shipped to a centralized refinery. To determine the feasibility of this approach, pelletized AFEX‐treated corn stover was hydrolyzed at high (18–36%) solid loadings. Water absorption and retention by the pellets was low compared to unpelletized stover, which allowed enzymatic hydrolysis slurries to remain well mixed without the need for fed‐batch addition. Glucose yields of 68% and xylose yields of 65% were obtained with 20 mg enzyme/g glucan and 18% solid loading after 72 h, compared to 61% and 59% for unpelletized corn stover. Pelletization also slightly increased the initial rate of hydrolysis compared to unpelletized biomass. The ease of mixing and high yields obtained suggests that pelletization after AFEX pretreatment could have additional advantages beyond improved logistical handling of biomass. Biotechnol. Bioeng. 2014;111: 264–271. © 2013 Wiley Periodicals, Inc.
A novel software framework for teaching aircraft dynamics and control
Authors: Shankar Praveen , Chung Wen‐Ting , Husman Jenefer , Wells Valana 
Published: August 2013

ABSTRACTAircraft dynamics and control (ADC) is a core course requirement for an undergraduate program in aeronautical engineering. This article presents an undertaking that reconfigures the instruction of fundamental concepts in ADC by replacing traditional problem‐solving approach with a method that relies on a simulation framework comprising of Matlab, USAF DATCOM, and flight simulation software. The typical challenges experienced by students including their inability to visualize complicated, multi‐modal aircraft were overcome by the new method thus enhancing the student's learning experience. The implemented approach improved students' motivational belief and its calibration, their use of active learning strategies and actual performance. © 2013 Wiley Periodicals, Inc. Comput Appl Eng Educ 23:63–71, 2015; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.21579
Characterization of a Cyclic Nucleotide‐Activated K+ Channel and its Lipid Environment by Using Solid‐State NMR Spectroscopy
Authors: Cukkemane Abhishek , Baldus Marc 
Published: August 2013

AbstractVoltage‐gated ion channels are large tetrameric multidomain membrane proteins that play crucial roles in various cellular transduction pathways. Because of their large size and domain‐related mobility, structural characterization has proved challenging. We analyzed high‐resolution solid‐state NMR data on different isotope‐labeled protein constructs of a bacterial cyclic nucleotide‐activated K+ channel (MlCNG) in lipid bilayers. We could identify the different subdomains of the 4×355 residue protein, such as the voltage‐sensing domain and the cyclic nucleotide binding domain. Comparison to ssNMR data obtained on isotope‐labeled cell membranes suggests a tight association of negatively charged lipids to the channel. We detected spectroscopic polymorphism that extends beyond the ligand binding site, and the corresponding protein segments have been associated with mutant channel types in eukaryotic systems. These findings illustrate the potential of ssNMR for structural investigations on large membrane‐embedded proteins, even in the presence of local disorder.
Effect of Organic Capping Agents on Ruthenium‐Nanoparticle‐Catalyzed Aqueous‐Phase Fischer–Tropsch Synthesis
Authors: Quek Xian‐Yang , Pestman Robert , van Santen Rutger A. , Hensen Emiel J. M. 
Published: August 2013

AbstractThe influence of organic capping agents on the performance of Ru nanoparticles in aqueous‐phase Fischer–Tropsch (FT) synthesis was investigated. Three organic capping agents were used: trimethyl(tetradecyl)ammonium bromide (TTAB), polyvinylpyrrolidone (PVP), and sodium 3‐mercapto‐1‐propanesulfonate (SMPS). To exclude the effects of particle size, the capping agents were placed onto carbon‐nanofiber‐supported Ru nanoparticles of size 3.4 nm. The activity in the FT reaction increased in the order: Ru‐SMPS≪Ru‐PVP<Ru‐TTAB<Ru. Kinetic data suggest that the FT mechanism was largely unaffected by the presence of capping agents; thus, their binding to active centers explains activity trends. Replacing water with n‐hexadecane as the solvent results in an increase in the rate of formation and a decrease in the chain‐growth probability for hydrocarbons, whereas the production of oxygenates is unaffected. This trend is consistent with the proposal that hydrocarbons are formed on reaction centers that involve facile CO dissociation and that termination is the rate‐determining step. For oxygenates, CO dissociation is proposed to be the rate‐determining step.
Electronic Structure Trends in N‐Heterocyclic Carbenes (NHCs) with Varying Number of Nitrogen Atoms and NHCTransition‐Metal Bond Properties
Authors: Bernhammer Jan C. , Frison Gilles , Huynh Han Vinh 
Published: August 2013

AbstractCarbenes derived from five‐membered heterocycles with different numbers of nitrogen atoms ranging from two to four lead formally either to normal N‐heterocyclic or mesoionic carbenes with, in some cases, the same skeletal structure. The electronic structures of fourteen of these compounds were examined by means of DFT calculations at the B3LYP/aug‐cc‐pVTZ level. The examined parameters include the energies of the σ‐lone pair at Ccarbene and the π‐HOMO of the protonated form, which are correlated to the first and second proton affinities. The singlet–triplet energy gap was used as a measure of the stability of the N‐heterocyclic carbene (NHC) towards dimerisation. Natural population analysis provided insight into the variation of the pπ population and the natural charge at Ccarbene with NHC structure. Additionally, the transition metalNHC bond in L‐AuCl and L‐TiCl4 and the nature of the orbital interactions between the NHC and the transition‐metal fragment were analysed in detail by the extended transition state–natural orbitals for chemical valence (ETS–NOCV) approach at the BP86/TZ2P level. Similarities and differences between the NHCgold and the NHCtitanium bond are discussed, and trends in key bonding properties can be traced back to the variation of the electronic parameters of the NHC.