bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimalisasi Personal Hygiene Menggosok Gigi untuk Early Diagnosis and Prompt Treatment Karies Gigi
Authors: Adimayanti Eka , Siyamti Dewi , Susilo Tri 
Published: August 2024

Karies gigi adalah kerusakan jaringan yang disebabkan oleh asam yang ada dalam karbohidrat melalui perantara mikroorganisme yang ada dalam saliva. Salah satu upaya pencegahan karies gigi adalah dengan mengajarkan cara menggosok gigi secara benar secara dini. menggosok gigi adalah membersihkan gigi dari sisa-sisa makanan, bakteri, dan plak. Kegiatan pengabdian kepada masyarakat ini berupa memberikan pengetahuan dan ketrampilan untuk mencegah penyakit secara dini terutama untuk kesehatan gigi dan mulut pada siswa TKIT Cahaya Ummat untuk mencegah lebih dini terjadinya karies gigi. Pengetahuan juga menjadi hal yang penting, terutama tentang cara menggosok gigi yang benar akan mampu mencegah terjadinnya masalah kesehatan mulut terutama masalah caries gigi.
ANALISIS PELAKSANAAN KEBIJAKAN MELALUI DISKRESI OLEH PEJABAT NEGARA
Authors: Parlindungan S Gokma Toni 
Published: August 2024

Referring to the discussed material above, several conclusions can be drawn regarding the internalization of the Rule of Law elements into efforts to hold accountable the abuse of authority in discretion (decisions and/or actions) by Government Officials related to corruption offenses involving financial losses to the state. This can be considered a rational thought result inseparable from the first influence, legal politics [outline or basic plan of government's agenda] in the UUAP related to the use of discretion by government bodies or officials in the form of legal policy or official guidelines containing the outline and basic plan of the government as the basis for the implementation of government administration itself. Second, the law enforcement process for officials suspected of committing corruption offenses involving financial losses to the state through the use of discretion must first adhere to the legal norms used by officials in their activities, namely administrative law which conceptually has principles, norms, and characteristics different from criminal law. Third, the issue of freedom to make policies (freis ermessen-discretionary power) falls within the domain of administrative law and thus cannot serve as a basis for criminal prosecution. Therefore, decision-makers and/or actions (discretion) cannot be easily criminalized, ensuring that government bodies and/or officials are not hindered in innovating government administration. Keywords: Analysis, Policy, Discretion, Government Officials.
O uso da rede social whatsapp® como ferramenta de educação em saúde
Authors: Fontana Rosane Teresinha , Wachekowski Giovana , Betana Cargnin Marcia , Amabile Guimaraes Carine 
Published: August 2024

Buscou-se avaliar a contribuição do uso de uma rede social como ferramenta de educação para a prevenção de agravos e promoção da saúde. Pesquisa de abordagem qualitativa, norteada pelo método netnográfico que teve como público-alvo, 21 estudantes do oitavo ano de uma escola privada de um município da região noroeste do estado do Rio Grande do Sul. A coleta de dados foi realizada no segundo semestre do ano de 2019 e ocorreu em três momentos distintos, utilizando-se a ferramenta Google Forms e o aplicativo WhatsApp®. O estudo respeitou os preceitos da pesquisa em seres humanos. Dos participantes, 81% nunca havia participado de uma atividade de educação em saúde. A temática de maior interesse dos adolescentes foi Infecções Sexualmente Transmissíveis As atividades de educação em saúde foram bem recebidas pelos estudantes, que foram participativos. A maioria relatou que a atividade foi importante para a sua aprendizagem e mais da metade afirmaram que buscariam o profissional enfermeiro para consultas e orientações, após esta atividade. Pode-se inferir que o WhatsApp pode ser utilizado pelos enfermeiros como uma ferramenta para aproximar adolescentes às atividades de educação em saúde.
O Curso Clássico do Colégio Estadual de Paranavaí na criação do Festival de Música e Poesia de Paranavaí
Authors: Rosa dos Santos Cruz Pessoa Edna Mara , Stentzler Márcia Marlene , De Fátima Henrique de Assis Eloah 
Published: August 2024

Resumo: Este estudo é resultado de pesquisa desenvolvida por meio de projeto de Iniciação Científica (PIC-EM) da Universidade Estadual do Paraná (Unespar), campus Paranavaí. O objetivo foi investigar a trajetória histórica do Festival de Música e Poesia de Paranavaí/PR (Femup) criado a partir da iniciativa de alunos do Curso Clássico do Colégio Estadual de Paranavaí, no ano de 1966. Desde então, o Femup é realizado anualmente, promovendo a disseminação de poesias, contos e músicas inéditas. Esta pesquisa oportunizou conhecer a historicidade desse festival e o contexto de sua criação associado à história da educação de Paranavaí. Analisamos publicações no jornal Diário do Noroeste, em blog, além das fontes documentais e registros fotográficos pertencentes ao acervo mantido pelo Colégio Estadual de Paranavaí. Resgatamos memórias, aspectos da história do Femup e da organização dos estudantes em sua formação.
Palavras-chave: Estudantes, festival, comunidade, música, poesia e contos.
Insetos como instrumento de aprendizagem no ensino de ciências
Authors: Layonn de Sousa Silva John , José Fontenele Oliveira Rômulo , Henrique da Silva Sousa Carlos 
Published: August 2024

Resumo: A grande quantidade existente dentro do leque entomológico, permite que os insetos sejam ferramentas de grande importância para o ensino de ciências como um todo, possibilitando interdisciplinaridade. Neste sentido, foi despertada a necessidade de buscar se os insetos são e como vêm sendo utilizados pelos docentes em sala de aula e no ensino de ciências do ensino base. Desta forma foi objetivado a utilização dos insetos como ferramenta de ensino aprendizagem. Foi optado por uma pesquisa qualitativa, revisão bibliográfica, artigos, periódico da CAPES, SCielo e repositórios acadêmicos de instituições públicas e, nacionais, de preferência a língua portuguesa, sobre utilização dos insetos no ensino de ciências, partindo de pontos de vista onde é exposto à visão de autores sobre a importância de trabalhar o saber entomológico no ensino, e o significado de ensinar de forma objetiva e científica, rompendo as limitações do ambiente de sala de aula e difundindo conceitual sobre o temática. Partiu-se da ideia de que os professores devem trabalhar os conteúdos, dentro de suas disciplinas e independente de qualquer que seja o conteúdo, buscar de forma interdisciplinar agregar conhecimentos sobre ciências. Concluímos que que a utilização entomológica como ferramenta de ensino, tem sido alvo de docentes no ensino de ciências, e que esta tática permite uma ampla abrangência no tocante ao aprendizado. Os artigos trabalhados apontam resultados de aceitação pelos discentes e ainda possibilita uma adequação cognitiva, dando aos mesmos uma nova perspectiva científica e que não se prende somente ao livro didático.
Palavras- chave: Insetos, Ensino, Ciências.
Percepção de licenciandos sobre as imagens: contribuições para a formação de professores
Authors: Oestreich Laura , Goldschmidt Andréa Inês 
Published: August 2024

Este artigo tem como objetivo investigar como licenciandos de Ciências da Natureza percebem o emprego de imagens e a sua influência nos processos de ensino e de aprendizagem, buscando subsidiar discussões e cursos de formação voltados para a temática. A pesquisa qualitativa, abrangeu um questionário realizado via Google Forms com 41 licenciandos. As questões foram analisadas de acordo com Análise de Conteúdo. Os resultados sinalizaram que para a maioria dos respondentes as imagens são sempre ou geralmente empregadas e buscadas principalmente na internet, cumprindo funções de facilitadoras do ensino-aprendizagem, além de tornar as aulas mais dinâmicas. São recursos autoexplicativos e embora a maioria sinalize que possui critérios para selecioná-las, uma parcela significativa, não os possui ou admitiu nunca ter pensado sobre, denotando uma visão ingênua e a necessidade da inserção deste tema no percurso formativo docente.
Performance of metagenomic Next-Generation Sequencing and metagenomic Nanopore Sequencing for the diagnosis of tuberculosis in HIV-positive patients
Authors: Yuan Jing , Wang Lanchun , Zhang Wei , Deng Changgang , Li Qisui , Meng Yamin , Chen Yaokai 
Published: August 2024

BackgroundPatients who were infected by the Human Immunodeficiency Virus (HIV) could have weakened immunity that is complicated by opportunistic infections, especially for Mycobacterium tuberculosis (MTB). Notably, the HIV-MTB co-infection will accelerate the course of disease progress and greatly increase the mortality of patients. Since the traditional diagnostic methods are time-consuming and have low sensitivity, we aim to investigate the performance of mNGS (metagenomic Next-Generation Sequencing) and mNPS (metagenomic NanoPore Sequencing) for the rapid diagnosis of tuberculosis in HIV-infected patients.MethodsThe 122 HIV-infected patients were enrolled for the retrospective analysis. All of the patients underwent traditional microbiological tests, mNGS, and (or) mNPS tests. The clinical comprehensive diagnosis was used as the reference standard to compare the diagnostic performance of culture, mNGS, and mNPS on tuberculosis. We also investigate the diagnostic value of mNGS and mNPS on mixed-infection. Furthermore, the treatment adjustment directed by mNGS and mNPS was analyzed.ResultsCompared with the composite reference standard, the culture showed 42.6% clinical sensitivity and 100% specificity, and the OMT(other microbiological testing) had 38.9% sensitivity and 100% specificity. The mNGS had 58.6% clinical sensitivity and 96.8% specificity, and the mNPS had 68.0% clinical sensitivity and 100% specificity. The proportion of mixed-infection cases (88.9%) in the TB group was higher than those in the non-TB group (54.8%) and the mNGS and mNPS are more competitive on mixed-infection diagnosis compared with the traditional methods. Furthermore, there are 63 patients (69.2%) and 36 patients (63.2%) achieved effective treatment after receiving the detection of mNPS and mNGS, respectively.ConclusionOur study indicated that mNPS and mNGS have high sensitivity and specificity for TB diagnosis compared with the traditional methods, and mNPS seems to have better diagnostic performance than mNGS. Moreover, mNGS and mNPS showed apparent advantages in detecting mixed infection. The mNPS and mNGS-directed medication adjustment have effective treatment outcomes for HIV-infected patients who have lower immunity.
A novel nomogram for predicting the recurrence of atrial fibrillation in patients treated with first-time radiofrequency catheter ablation for atrial fibrillation
Authors: Ma Guiling , Zou Changhong , Zhang Zhiyong , Zhang Lin , Zhang Jianjun 
Published: August 2024

IntroductionThe purpose of this study was to investigate the predictive factors of atrial fibrillation (AF) recurrence in patients after first-time radiofrequency catheter ablation (RFCA) and to develop a nomogram predictive model that can provide valuable information for determining the ablation strategy.MethodsIn total, 500 patients who had received first-time RFCA for AF were retrospectively enrolled in the study. The patients were divided into a training cohort (n = 300) and a validation cohort (n = 200) randomly at a 6:4 ratio. Lasso and multivariate logistic regression analyses were used to screen the predictors for AF recurrence during a 2-year follow-up. The C-index and a calibration plot were used to detect the discriminative ability and calibration of the nomogram. The performance of the nomogram was assessed compared with the APPLE score, CAAP-AF score, and MB-LATER score using the receiver operating characteristic (ROC) curve, decision curve analysis (DCA), integrated discrimination index (IDI), and net reclassification index (NRI).ResultsA total of 78 patients experienced the recurrence of AF after first-time RFCA in the training cohort. The six strongest predictors for AF recurrence in the training cohort were persistent AF, duration of AF, left atrial diameter (LAD), estimated glomerular filtration rate (eGFR), N-terminal pro-brain natriuretic peptide (NT-proBNP), and autoantibody against M2-muscarinic receptor (anti-M2-R). Based on the above six variables, a nomogram prediction model was constructed with a C-index of 0.862 (95% CI, 0.815–0.909), while the C-index was 0.831 (95% CI, 0.771–0.890) in the validation cohort. DCA showed that this nomogram had greater net benefits compared with other models. Furthermore, the nomogram showed a noticeable improvement in predictive performance, sensitivity, and reclassification for AF recurrence compared with the APPLE score, CAAP-AF score, or MB-LATER score.ConclusionWe established a novel predictive tool for AF recurrence after the first-time RFCA during a 2-year follow-up period that could accurately predict individual AF recurrence.
The left axillary artery is a reasonable option as the inflow site for saphenous vein graft in minimally invasive coronary artery bypass grafting
Authors: Ushioda Ryohei , Hirofuji Aina , Yoongtong Dit , Sakboon Boonsap , Cheewinmethasiri Jaroen , Lokeskrawee Thanin , Patumanond Jayanton , Lawanaskol Suppachai , Kamiya Hiroyuki , Arayawudhikul Nuttapon 
Published: August 2024

IntroductionThis study aims to clarify the good inflow site for saphenous vein grafts (SVG) in minimally invasive off-pump coronary artery bypass grafting (mini-CABG), between the ascending aorta, the internal thoracic arteries (ITAs) and the left axillary artery (LAA).MethodsThis retrospective study included 126 patients who underwent Mini-CABG at our center between January 2014 and July 2023. Patients were divided into three groups according to the SVG inflow site for patency comparison: Aorta group (n = 56), LAA group (n = 23), and ITA group (n = 47).ResultsThere were 84 males, with mean age of 65.9 ± 7.0 years. There were no significant differences in preoperative characteristics between groups. Mean operation times were 254.6 ± 72.2, 213.7 ± 57.6, and 253.0 ± 81.2 min, and the average numbers of distal anastomoses were 2.9 ± 0.9, 2.4 ± 0.7 and 2.9 ± 1.1 in the Aorta, ITA and LAA groups respectively. Days in intensive care, hospital stay, and major complications did not differ between the groups. Early patency of SVG did not significantly differ among groups: 93.0% in the Aorta group, 98.0% in the ITA group, and 100% in the LAA group. Mean follow-up period was 136.7 ± 295.7 days, and follow-up coronary CTA revealed 18 SVG occlusions (Aorta group n = 8, ITA group n = 5, LAA group n = 5). The Kaplan-Meier curve for SVG patency rates did not show any significant differences among the three groups.ConclusionThe ascending aorta, the ITAs, and the LAA serve as reliable inflow sites with similar results in mini-CABG.
Co-creating academic career self-efficacy: exploring academic career segments, mediating and moderating influences in engineering education trajectory
Authors: Mahajan Prashant 
Published: August 2024

IntroductionThis study explores the dynamic relationship among different academic career stages within engineering education, using academic career self-efficacy (ACSE) – one’s belief in their academic ability, as the unitary construct. The purpose of this study was to examine the relationships between the academic career segments demonstrating self-efficacy at the beginning of career, during academic progression, and post-graduation completion, along with the mediating and moderating effects corresponding to the relationships.MethodsQuantitative survey was conducted on Indian recent engineering graduates. Data was collected from closed-ended questionnaire. Structural equation modeling was employed to analyze the relationships among academic career segments, mediation and the moderating impact of personal traits.ResultsThere were statistical significances among the relationships between academic career segments with each other. Notably, ACSE during academic progression emerged as a crucial mediator between entry-level ACSE and ACSE post-graduation completion. Furthermore, gender, social caste, engineering major, campus placement and engineering grade were identified as significant moderators for these relationships.DiscussionIt sheds light on the significance of ACSE throughout diverse academic career segments enhancing career aspirations, engineering skills, and expressions and reflections that facilitate collaborative co-creation for engineering as a career. It contributes to the positive propagation of engineering education as an excellent and rewarding career choice.
Inflammatory cytokine expression in Fabry disease: impact of disease phenotype and alterations under enzyme replacement therapy
Authors: Yuan Yujing , Zhao Yawen , Li Fan , Ling Chen , Wu Yuan , Ma Wei , Wang Zhaoxia , Yuan Yun , Hao Hongjun , Zhang Wei 
Published: August 2024

ObjectivesThe aim of this study is to explore the expression of inflammatory cytokines (ICs) in Fabry disease (FD), the correlation between ICs and FD phenotypes, and the impact of enzyme replacement therapy (ERT) on IC expression.MethodsWe recruited 67 FD patients and 44 healthy controls (HCs) and detected concentrations of the following ICs: interferon-γ, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12P70, IL-17A, IL-17F, IL-22, tumor necrosis factor (TNF)-α, and TNF-β. We also analyzed the impact of ERT on IC expression in FD patients and the relationship between IC expression and sex, genotype, phenotype, disease burden, and biomarkers.ResultsMost ICs were significantly higher in FD patients than in HCs. A number of ICs were positively correlated with clinical aspects, including disease burden (Mainz Severity Score Index [MSSI]) and cardiac and renal markers. IL-8 was higher in the high MSSI (P-adj=0.026*) than in the low MSSI.ConclusionsICs were upregulated in FD patients, indicating the role of the innate immune process in FD etiology. ERT ameliorated FD-related inflammatory activation, at least to some extent. IC expression was positively correlated with disease burden and clinical markers in FD. Our findings indicated that the inflammatory pathway may be a promising therapeutic target for FD.
Integrating single-cell transcriptomics to reveal the ferroptosis regulators in the tumor microenvironment that contribute to bladder urothelial carcinoma progression and immunotherapy
Authors: Chen Ziang , Hu Jia , Ou Yuxi , Ye Fangdie , Li Weijian , Liu Shenghua , Jiang Haowen 
Published: August 2024

BackgroundFerroptosis, as a novel form of programmed cell death, plays a crucial role in the occurrence and development of bladder cancer (BCa). However, the regulatory mechanisms of ferroptosis in the tumor microenvironment (TME) of BCa remain to be elucidated.MethodsBased on single-cell RNA (scRNA) transcriptomic data of BCa, we employed non-negative matrix factorization (NMF) dimensionality reduction clustering to identify novel ferroptosis-related cell subtypes within the BCa TME, aiming to explore the biological characteristics of these TME cell subtypes. Subsequently, we conducted survival analysis and univariate Cox regression analysis to explore the prognostic significance of these cell subtypes. We investigated the relationship between specific subtypes and immune infiltration, as well as their implications for immunotherapy. Finally, we discovered a valuable and novel biomarker for BCa, supported by a series of in vitro experiments.ResultsWe subdivided cancer-associated fibroblasts (CAFs), macrophages, and T cells into 3-5 small subpopulations through NMF and further explored the biological features. We found that ferroptosis played an important role in the BCa TME. Through bulk RNA-seq analysis, we further verified that ferroptosis affected the progression, prognosis, and immunotherapy response of BCa by regulating the TME. Especially ACSL4+CAFs, we found that high-level infiltration of this CAF subtype predicted worse prognosis, more complex immune infiltration, and less response for immunotherapy. Additionally, we found that this type of CAF was associated with cancer cells through the PTN-SDC1 axis, suggesting that SDC1 may be crucial in regulating CAFs in cancer cells. A series of in vitro experiments confirmed these inferences: SDC1 promoted the progression of BCa. Interestingly, we also discovered FTH1+ macrophages, which were closely related to SPP1+ macrophages and may also be involved in the regulation of BCa TME.ConclusionThis study revealed the significant impact of ferroptosis on bladder cancer TME and identified novel ferroptosis-related TME cell subpopulations, ACSL4+CAFs, and important BCa biomarker SDC1.
Deciphering the causal association and underlying transcriptional mechanisms between telomere length and abdominal aortic aneurysm
Authors: Zhang Jiyu , Xia Xinyi , He Shujie 
Published: August 2024

BackgroundThe purpose of this study is to investigate the causal effect and potential mechanisms between telomere length and abdominal aortic aneurysm (AAA).MethodsSummary statistics of telomere length and AAA were derived from IEU open genome-wide association studies and FinnGen R9, respectively. Bi-directional Mendelian randomization (MR) analysis was conducted to reveal the causal relationship between AAA and telomere length. Three transcriptome datasets were retrieved from the Gene Expression Omnibus database and telomere related genes was down-loaded from TelNet. The overlapping genes of AAA related differentially expressed genes (DEGs), module genes, and telomere related genes were used for further investigation. Telomere related diagnostic biomarkers of AAA were selected with machine learning algorisms and validated in datasets and murine AAA model. The correlation between biomarkers and immune infiltration landscape was established.ResultsTelomere length was found to have a suggestive negative associations with AAA [IVW, OR 95%CI = 0.558 (0.317-0.701), P &lt; 0.0001], while AAA showed no suggestive effect on telomere length [IVW, OR 95%CI = 0.997 (0.990-1.004), P = 0.4061]. A total of 40 genes was considered as telomere related DEGs of AAA. PLCH2, PRKCQ, and SMG1 were selected as biomarkers after multiple algorithms and validation. Immune infiltration analysis and single cell mRNA analysis revealed that PLCH2 and PRKCQ were mainly expressed on T cells, while SMG1 predominantly expressed on T cells, B cells, and monocytes. Murine AAA model experiments further validated the elevated expression of biomarkers.ConclusionWe found a suggestive effect of telomere length on AAA and revealed the potential biomarkers and immune mechanism of telomere length on AAA. This may shed new light for diagnosis and therapeutics on AAA