bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in signal molecules and maturation promoting factor levels associate with spontaneous resumption of meiosis in rat oocytes
Authors: Prasad Shilpa , Tiwari Meenakshi , Tripathi Anima , Pandey Ashutosh N. , Chaube Shail K. 
Published: January 2015

AbstractThe present study was aimed to find out changes in signal molecules and maturation promoting factor (MPF) levels during meiotic cell cycle progression from diplotene and metaphase‐II (M‐II) arrest, a period during which oocyte achieves meiotic competency. Data suggest that high levels of adenosine 3′‐5′‐cyclic monophosphate (cAMP), guanosine 3′‐5′‐cyclic monophosphate (cGMP), and nitric oxide (NO) are associated with diplotene arrest, while reduction in their levels correlates with reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increased intracellular NO, calcium (Ca2+) as well as hydrogen peroxide (H2O2) levels correlate with decreased cAMP, Thr‐161 phosphorylated cyclin‐dependent kinase‐1 (Cdk1) as well as cyclin B1 levels. The decreased Thr‐161 phosphorylated Cdk1 and cyclin B1 level reduce MPF level leading to exit from M‐II arrest in oocytes cultured in vitro. These data suggest that the decrease of cAMP level and increase of cytosolic free Ca2+ as well as H2O2 levels associate with the reduced MPF level and meiotic resumption from diplotene arrest. On the other hand, increase of NO, cGMP, Ca2+ as well as H2O2 levels are associated with reduced MPF and spontaneous exit from M‐II arrest in rat oocytes cultured in vitro.
Palladium‐Catalyzed Enantioselective Allylic Substitution in the Presence of Monodentate Furanoside Phosphoramidites
Authors: Majdecki Maciej , Jurczak Janusz , Bauer Tomasz 
Published: January 2015

AbstractA library of monodentate furanoside phosphoramidites, easily synthesized from inexpensive D‐xylose and optically pure 1,1′‐bi‐2‐naphthol (BINOL), was used as ligands for the palladium‐catalyzed allylic alkylation and amination. The matched pair was formed from D‐xylose‐derivatives and (S)‐BINOL. The asymmetric induction depends strongly on the substituent at the C5 of the carbohydrate backbone; both bulky 5‐O‐pivaloyl and 5‐deoxy derivatives gave excellent results, whereas ligands with trityl protection at position C5 induced low ee values with reversal of configuration. The solvent used for the addition is also of great importance with highest enantioselectivities observed in diethyl ether. The best results for both alkylation and amination, up to 98–99 % ee, were obtained for sterically demanding allylic acetates.
Discrete Modeling of Growth of Hairy Roots in a Mist Bioreactor
Authors: Ranjan Ritu , Katuri Sambasiva Rao , Khanna Rajesh 
Published: January 2015

AbstractHairy root culture is a promising route for large‐scale secondary metabolite production. A discrete model is developed to study the kinetic growth of these valuable roots in a reactor. The growth process is defined to occur in three sequential phases. The elongation rate is modeled as exponential growth with the growth coefficient being dependent on mass transfer coefficient, nutrient concentration difference, and distribution of nutrients in growth and sustenance requirements. Results indicate that the primary root growth is reduced by one‐fifth of its initial growth rate due to the branching process, and the growth of new branches is significantly faster than its primary root growth due to internal transport of nutrients. The model is successfully validated against experimental findings. Its integration with spatio‐temporal variation of nutrients in a reactor will be a crucial input to large‐scale production of hairy roots.
Bed Expansion and Particle Classification in Liquid Fluidized Beds with Structured Internals
Authors: Piovano Stella , Salierno Gabriel L. , Montmany Emiliano , D'Agostino Mauro , Maestri Mauricio , Cassanello Miryan 
Published: January 2015

AbstractThe inclusion of a structured packing as internal in a liquid‐solid fluidized bed allows expansion of the liquid velocity operation range before elutriation, promoting the liquid solid contact and mixing. The bed expansion of liquid‐solid fluidized beds provided with structured packing as internals is examined, for solids denser than the liquid phase and within a wide range of operating conditions. A correlation to estimate the bed expansion in liquid‐solid fluidized beds using structured packing as internals is developed. In addition, the feasibility of employing structured packing as internals for favoring classification of different density particles is demonstrated by analyzing the mass elutriated from the column at different liquid velocities for single particles or binary mixtures.
A Metalloenzyme‐Like Catalytic System for the Chemoselective Oxidative Cross‐Coupling of Primary Amines to Imines under Ambient Conditions
Authors: Largeron Martine , Fleury Maurice‐Bernard 
Published: January 2015

AbstractThe direct oxidative cross‐coupling of primary amines is a challenging transformation as homocoupling is usually preferred. We report herein the chemoselective preparation of cross‐coupled imines through the synergistic combination of low loadings of CuII metal‐catalyst and o‐iminoquinone organocatalyst under ambient conditions. This homogeneous cooperative catalytic system has been inspired by the reaction of copper amine oxidases, a family of metalloenzymes with quinone organic cofactors that mediate the selective oxidation of primary amines to aldehydes. After optimization, the desired cross‐coupled imines are obtained in high yields with broad substrate scope through a transamination process that leads to the homocoupled imine intermediate, followed by dynamic transimination. The ability to carry out the reactions at room temperature and with ambient air, rather than molecular oxygen as the oxidant, and equimolar amounts of each coupling partner is particularly attractive from an environmentally viewpoint.
Asymmetric Conjugate Additions and Allylic Alkylations Using Nucleophiles Generated by Hydro‐ or Carbometallation
Authors: Maksymowicz Rebecca M. , Bissette Andrew J. , Fletcher Stephen P. 
Published: January 2015

AbstractThis Minireview discusses catalytic asymmetric conjugate addition and allylic alkylation reactions where the nucleophiles were generated in situ by hydrometallation or carbometallation. This exciting recent trend in asymmetric catalysis promises to expand the range of transformations available for the rapid and selective assembly of complex, functional molecules for both academic and industrial research. This Minireview aims to serve as a reference for studies reported to date and discusses the current state‐of‐the‐art, scope and limitations of these processes.
Deprotonation of Coordinated Phosphanes in a Rhenium Complex: CC Coupling with Diimine Coligands
Authors: Arévalo Rebeca , Pérez Julio , Riera Lucía 
Published: January 2015

AbstractThe reaction of fac‐[Re(bipy)(CO)3(PMe3)][OTf] (bipy=2,2′‐bipyridine) with KN(SiMe3)2 affords two neutral products: cis,trans‐[Re(bipy)(CO)2(CN)(PMe3)], and a thermally unstable compound, which features a new CC bond between a P‐bonded methylene group (from methyl group deprotonation) and the C6 position of bipy. The solid‐state structures of more stable 1,2‐bis[(2,6‐diisopropylphenyl)imino]acenaphthene analogs, resulting from the deprotonation of PMe3, PPhMe2, and PPh2Me ligands, are determined by X‐ray diffraction.
General and Mild Ni0‐Catalyzed α‐Arylation of Ketones Using Aryl Chlorides
Authors: Fernández‐Salas José A. , Marelli Enrico , Cordes David B. , Slawin Alexandra M. Z. , Nolan Steven P. 
Published: January 2015

AbstractA general methodology for the α‐arylation of ketones using a nickel catalyst has been developed. The new well‐defined [Ni(IPr*)(cin)Cl] (1 c) pre‐catalyst showed great efficiency for this transformation, allowing the coupling of a wide range of ketones, including acetophenone derivatives, with various functionalised aryl chlorides. This cinnamyl‐based Ni–N‐heterocyclic carbene (NHC) complex has demonstrated a different behaviour to previously reported NHC‐Ni catalysts. Preliminary mechanistic studies suggest a Ni0/NiII catalytic cycle to be at play.
Paclitaxel/carboplatin with or without belinostat as empiric first‐line treatment for patients with carcinoma of unknown primary site: A randomized, phase 2 trial
Authors: Hainsworth John D. , Daugaard Gedske , Lesimple Thierry , Hübner Gerdt , Greco F. Anthony , Stahl Michael J. , Büschenfelde Christian Meyer Zum , Allouache Djelila , Penel Nicolas , Knoblauch Poul , Fizazi Karim S. 
Published: January 2015

BACKGROUNDThe objective of this study was to evaluate the efficacy of belinostat, a histone deacetylase inhibitor, when added to paclitaxel/carboplatin in the empiric first‐line treatment of patients with carcinoma of unknown primary site (CUP).METHODSIn this randomized phase 2 trial, previously untreated patients with CUP were randomized to receive belinostat plus paclitaxel/carboplatin (group A) or paclitaxel/carboplatin alone (group B) repeated every 21 days. Patients were re‐evaluated every 2 cycles, and those without disease progression continued treatment for 6 cycles. Patients in group A then continued receiving single‐agent belinostat, whereas patients in group B stopped treatment. The primary endpoint was progression‐free survival (PFS): The authors postulated that the addition of belinostat would improve PFS from 5 months (expected with paclitaxel/carboplatin) to 8 months.RESULTSIn total, 89 patients were randomized (group A, n = 44; group B, n = 45), and the demographics and disease characteristics were balanced between the 2 groups. The addition of belinostat to paclitaxel/carboplatin did not improve PFS (group A, 5.4 months [95% confidence interval, 3.0‐6.0 months]; group B, 5.3 months [95% confidence interval, 2.8‐6.6 months]; P = .85). Overall survival was 12.4 months for group A versus 9.1 months for group B (P = .20). The response rate favored the belinostat group (45% vs 21%; P = .02). Belinostat resulted in a modest increase in treatment toxicity.CONCLUSIONSThe addition of belinostat to paclitaxel/carboplatin did not improve the PFS of patients with CUP who were receiving first‐line therapy, although the patients who received belinostat had a higher investigator‐assessed response rate. Future trials in CUP should focus on specific subsets, defined either by the predicted tissue of origin or by the identification of targetable molecular abnormalities. Cancer 2015;121:1654–1661. © 2015 American Cancer Society.
Double‐Chamber Microbial Fuel Cell with a Non‐Platinum‐Group Metal Fe–N–C Cathode Catalyst
Authors: Santoro Carlo , Serov Alexey , Narvaez Villarrubia Claudia W. , Stariha Sarah , Babanova Sofia , Schuler Andrew J. , Artyushkova Kateryna , Atanassov Plamen 
Published: January 2015

AbstractNon‐Pt‐group metal (non‐PGM) materials based on transition metal–nitrogen–carbon (M–N–C) and derived from iron salt and aminoantipyrine (Fe‐AAPyr) of mebendazole (Fe‐MBZ) were studied for the first time as cathode catalysts in double‐chamber microbial fuel cells (DCMFCs). The pH value of the cathode chamber was varied from 6 to 11 to elucidate the activity of those catalysts in acidic to basic conditions. The Fe‐AAPyr‐ and Fe‐MBZ‐based cathodes were compared to a Pt‐based cathode used as a baseline. Pt cathodes performed better at pH 6–7.5 and had similar performances at pH 9 and a substantially lower performance at pH 11 at which Fe‐AAPyr and Fe‐MBZ demonstrated their best electrocatalytic activity. The power density achieved with Pt constantly decreased from 94–99 μW cm−2 at pH 6 to 55–57 μW cm−2 at pH 11. In contrast, the power densities of DCMFs using Fe‐AAPyr and Fe‐MBZ were 61–68 μW cm−2 at pH 6, decreased to 51–58 μW cm−2 at pH 7.5, increased to 65–75 μW cm−2 at pH 9, and the highest power density was achieved at pH 11 (68–80 μW cm−2). Non‐PGM cathode catalysts can be manufactured at the fraction of the cost of the Pt‐based ones. The higher performance and lower cost indicates that non‐PGM catalysts may be a viable materials choice in large‐scale microbial fuel cells.
Iron‐Catalyzed Hydrogenation of Bicarbonates and Carbon Dioxide to Formates
Authors: Zhu Fengxiang , Zhu‐Ge Ling , Yang Guangfu , Zhou Shaolin 
Published: January 2015

AbstractThe catalytic hydrogenation of carbon dioxide and bicarbonate to formate has been explored extensively. The vast majority of the known active catalyst systems are based on precious metals. Herein, we describe an effective, phosphine‐free, air‐ and moisture‐tolerant catalyst system based on Knölker’s iron complex for the hydrogenation of bicarbonate and carbon dioxide to formate. The catalyst system can hydrogenate bicarbonate at remarkably low hydrogen pressures (1–5 bar).
Atypical presentation of calvarial metastasis of renal cell carcinoma in an adolescent: A rare case diagnosed on fine needle aspiration cytology with literature review
Authors: Gaur Kavita , Mandal Shramana , Gondal Ranjana , Singh Daljit 
Published: January 2015

Renal cell carcinoma (RCC) is a complex and challenging neoplasm both in terms of treatment as well as diagnosis. Its unpredictable biological behavior and many deceptive appearances can sometimes bewilder the pathologist. We hereby report a rare case of a 17 year old girl presenting with an occipital swelling and importantly no prior documents at the time of aspiration. Fine needle aspiration cytology showed features of metastatic carcinoma with the following possibilities; metastatic RCC, melanoma, and hepatocellular carcinoma. Further detailed history of the patient and investigations were advised. Intraoperative squash smears also showed similar features. Histopathology of the lesion showed features of metastatic RCC.This case highlights the fact that a skull lesion in rare cases may be the presenting sign of an underlying malignancy. In the absence of key documents, providing the correct diagnosis may become very challenging and cytomorphology alone can be extremely helpful. Diagn. Cytopathol. 2015;43:412–415. © 2015 Wiley Periodicals, Inc.
Comorbidity Between Lifetime Eating Problems and Mood and Anxiety Disorders: Results from the Canadian Community Health Survey of Mental Health and Well‐being
Authors: Meng Xiangfei , D'Arcy Carl 
Published: January 2015

AbstractThis study was to examine profiles of eating problems (EPs), mood and anxiety disorders and their comorbidities; explore risk patterns for these disorders; and document differences in health service utilization in a national population. Data were from the Canadian Community Health Survey of Mental Health and Well‐being. The lifetime prevalence of EPs was 1.70% among Canadians, compared with 13.25% for mood disorder, 11.27% for anxiety disorder and 20.16% for any mood or anxiety disorder. Almost half of those with EPs also suffered with mood or anxiety disorders. A similar pattern in depressive symptoms was found among individuals with major depression and EPs, but individuals with EPs reported fewer symptoms. Factors associated with the comorbidity of EPs and mood and anxiety disorders were identified. Individuals with EPs reported more unmet needs. Patients with EPs should be concomitantly investigated for mood and anxiety disorders, as similar interventions may be effective for both. Copyright © 2015 John Wiley & Sons, Ltd and Eating Disorders Association.
Combined solar heat and power with microgrid storage and layered smartgrid control toward supplying off‐grid rural villages
Authors: Prinsloo Gerro , Dobson Robert 
Published: January 2015

AbstractA project has been initiated in South Africa to design, model, build, and evaluate an easy to install solar fueled combined heat and power (micro‐CHP) system to supply off‐grid rural villages and eco‐estate communities. This study describes the design of a model that offers a combined 3 kW peak electrical, 12 kW peak thermal, stand‐alone solar power technology solution with microgrid storage to deliver power, and heat when the sun is shining and after sunset (at night time). The CHP demand side management system manages power and heat distribution to individual households in a small rural village based on a proposed smart microgrid technology solution. The solution aims to balance the heat/power demand side requirements based on digital feedback on heat/power utilization patterns and user demand. Aspects of the micro‐CHP system microgrid control are discussed while a modular smartgrid solution is selected for smart microgrid applications.
Calcium and phosphate release from resin‐based materials containing different calcium orthophosphate nanoparticles
Authors: Rodrigues Marcela C. , Natale Livia C. , Arana‐Chaves Victor E. , Braga Roberto R. 
Published: January 2015

AbstractThe study compared ion release from resin‐based materials containing calcium orthophosphates. Amorphous calcium phosphate (ACP), dicalcium phosphate anhydrous (DCPA), dicalcium phosphate dihydrate (DCPD), and tricalcium phosphate (β‐TCP) nanoparticles were characterized by X‐ray diffraction (XRD), transmission electron microscopy (TEM), dynamic light scattering (DLS), and surface area (nitrogen adsorption isotherms, BET method). Nanoparticles were added to a dimethacrylate‐based resin and materials were tested for degree of conversion (DC) and calcium/phosphate release up to 28 days under pH 5.5 and 7.0. Data were analyzed by ANOVA/Tukey test (alpha: 0.05).The crystallinity of DCPA, DCPD, and β‐TCP were confirmed, as well as the ACP amorphous nature. DCPD and β‐TCP presented larger agglomerates than DCPA and ACP. The surface area of ACP was 5–11 times higher than those of the other nanoparticles. Materials showed similar DC. The material containing ACP released significantly more ions than the others, which released similar amounts of calcium and, in most cases, phosphate. Ion release was not affected by pH. Calcium release decreased between 7 and 21 days, while phosphate levels remained constant after 14 days. In conclusion, ACP higher ion release can be ascribed to its high surface area. DCPA, DCPD, and β‐TCP had similar performances as ion‐releasing fillers. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1670–1678, 2015.
Macroscopic, histochemical, and immunohistochemical comparison of hysterorrhaphy using catgut and chitosan suture wires
Authors: Huaixan Lucio N. , Arruda Silvana S. B. , Leonardo André S. , Viana Janiny C. , Barreto‐Vianna André R. C. , Ximenes Fábio H. B. , Agreste Fernanda R. , Godoy Roberta F. , Lima Eduardo M. M. 
Published: January 2015

AbstractThe objective of the current article was to compare the quality of the healing process after hysterorrhaphy with catgut and chitosan suture wires via macroscopic, histochemical, and immunohistochemical evaluations. Seven ewes were submitted to a video‐assisted laparotomy. A hysterectomy was performed with subsequent catgut and chitosan hysterorrhaphy in both horns. Then, macroscopic evaluation and biopsies of the hysterorrhaphy and control areas were carried out after 30 days. Although postoperative adhesion was observed in five animals of the catgut group, no adhesions were detected in the chitosan one. Additionally, there was a significant difference in increasing collagen type III and connective tissue for the chitosan group when compared to the control and catgut groups. A larger amount of blood vessels was also observed in the control group in comparison with the catgut and chitosan groups. Collagen type I and vascular endothelial growth factor‐A (VEGF‐A) were significantly different among groups. Thus, chitosan promoted a preventive effect on postoperative adhesion formation in the hysterorrhaphy areas, with increasing collagen type III deposition during tissue mending, which granted an enhanced healing process. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 50–57, 2016.
Effects of trypsinization and mineralization on intrasynovial tendon allograft healing to bone
Authors: Qu Jin , van Alphen Nick A. , Thoreson Andrew R. , Chen Qingshan , An Kai‐Nan , Amadio Peter C. , Schmid Thomas M. , Zhao Chunfeng 
Published: January 2015

ABSTRACTThe purpose of the current study was to develop a novel technology to enhance tendon‐to‐bone interface healing by trypsinizing and mineralizing (TM) an intrasynovial tendon allograft in a rabbit bone tunnel model. Eight rabbit flexor digitorum profundus (FDP) tendons were used to optimize the trypsinization process. An additional 24 FDP tendons were stratified into control and TM groups; in each group, 4 tendons were used for in vitro evaluation of TM and 8 were transplanted into proximal tibial bone tunnels in rabbits. The samples were evaluated histologically and with mechanical testing at postoperative week 8. Maximum failure strength and linear stiffness were not significantly different between the control and TM tendons. A thin fibrous band of scar tissue formed at the graft‐to‐bone interface in the control group. However, only the TM group showed obvious new bone formation inside the tendon graft and a visible fibrocartilage layer at the bone tunnel entrance. This study is the first to explore effects of TM on the intrasynovial allograft healing to a bone tunnel. TM showed beneficial effects on chondrogenesis, osteogenesis, and integration of the intrasynovial tendon graft, but mechanical strength was the same as the control tendons in this short‐term in vivo study. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:468–474, 2015.
Salinity thresholds and genotypic variability of cabbage (Brassica oleracea L.) grown under saline stress
Authors: Sanoubar Rabab , Cellini Antonio , Veroni Anna Maria , Spinelli Francesco , Masia Andrea , Vittori Antisari Livia , Orsini Francesco , Gianquinto Giorgio 
Published: January 2015

AbstractBACKGROUNDTwo botanical varieties of cabbage, namely Savoy (Brassica oleracea var. Sabauda L.) and White (Brassica oleracea var. Capitata L.) were used in order to understand the morphological, physiological and biochemical elements of functional salt stress response. Thirteen salt concentrations (range, 0 to 300 mmol L−1 NaCl) were considered in Experiment 1 and, of these 13, three (0, 100 and 200 mmol L−1 NaCl) were used in Experiment 2.RESULTSExperiment 1 enabled the definition of two salinity thresholds (100 and 200 mmol L−1 NaCl), associated with morphological and physiological adaptations. In Experiment 2, moderate salinity (100 mmol L−1 NaCl) had lower effects on Savoy than in White cabbage yield (respectively, −16% and −62% from control). Concurrently, 100 mmol L−1 NaCl resulted in a significant increase of antioxidant enzymes from control conditions, that was greater in Savoy (+289, +423 and +88%, respectively) as compared to White (+114, +356 and +28%, respectively) cabbage. Ion accumulation was found to be a key determinant in tissue osmotic adjustment (mainly in Savoy) whereas the contribution of organic osmolites was negligible.CONCLUSIONSHigher antioxidative enzymatic activities in Savoy versus White cabbage after treatment with 100 mmol L−1 NaCl were associated with improved water relations, thus suggesting a possible physiological pathway for alleviating perceived salt stress. © 2015 Society of Chemical Industry