bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlaciones moleculares, clínicas y serológicas de los alfaherpesvirus de Rumiantes, con especial referencia al HVB-1
Authors: Zapata Juan C , Bedoya Gabriel B , Ossa Jorge E , Zuluaga Fabio Nelson 
Published: August 2024

Resumen A pesar de que se tiene la secuencia genómica completa del HVB-1 (prototipo de estudio de los Alfaherpersvirus de rumiantes), hasta el momento, las relaciones entre los Alfaherpesvirus han sido pobremente caracterizadas. En este artículo, queremos revisar algunos trabajos que han comparado desde el punto de vista estructural y de secuencia, los ácidos nucleicos de este grupo de virus. También analizamos, algunos estudios sobre patogénesis, haciendo referencia especial al HVB-1, con énfasis en la descripción de variantes de este agente con diferentes grados de virulencia. Finalmente, se discute la posibilidad de que en Colombia, donde no hemos demostrado una lata evidencia clínica ni virológica, pero si una alta seroprevalencia, circules cepas relativamente apatógenas, como se ha propuesto desde hace varios años. Summary Although the complete sequence of the HVB-1 (prototype of study if the Alfaherpesviruses of ruminants) is available, the relationship among the alfaherpesvirus has been poorly characterized. This becomes an obstacle for the differential diagnosis if this viruses and for the filogenetic analysis among them. In this review, we want to present some of the information which has been published recently on genomic, clinic and serological comparisons among the agents of this viral group, with special emphasis on BHV-1. The data presented are compatible with our hypothesis that IBR strains circulating in Colombia may be less virulent.
Evaluación anatomopatológica de riñones lepóridos y determinación de la prevalencia de encefalitozoonosis en la hacienda El Progreso de la Universidad de Antioquia
Authors: Rodriguez Bernardo J. , Cañas Liliam , Zapata Marcela , Alvarez Lyda C. , Rico Silvia , Garay Francisco J. 
Published: August 2024

Resumen La encefalitozoonosis es una enfermedad zoonótica emergente causada por el microsporidio Encephalitozoon cuniculi, el cual se diagnosticó recientemente por primera vez en el departamento de Antioquia. Con el objetivo de determinar la prevalencia de esta enfermedad y caracterizar las lesiones renales, se realizó el estudio anatomopatológico de los riñones de 100 conejos asintomáticos al momento del sacrificio en la Hacienda El Progreso de la Universidad de Antioquia. El diagnóstico se basó en los hallazgos macroscópicos de las lesiones renales y la histopatología utilizando la coloración de rutina de Hematoxilina - Eosina y la confirmación del parásito mediante las coloraciones especiales de Sandiford, Ziehl - Neelsen, y Warthin - Starry. El análisis estadístico se efectuó utilizando estadística descriptiva y la prueba de independencia y contingencia de Chi cuadrado para establecer el grado de asociación entre algunas variables en estudio. La prevalencia de la encefalitozoonosis en los conejos fue del 5% y estuvo siempre relacionada con la presentación de nefritis intersticial por lo que se concluye que ésta enfermedad tiene una alta rata de presentación de la forma subclínica y que en consecuencia en el departamento de Antioquia existe una nueva enfermedad emergente afectando a la industria cunícola, constituyéndose en un factor de riesgo potencial para la salud pública. Palabras clave: conejo, Encephalitozoon cuniculi, nefritis intersticial, riñón. Summary Encephalitozoonosis is an emmerging zoonotic disease caused by the microsporidio Encephalitozoon cuniculi which was recently diagnosticated for the first time in the province of Antioquia (Colombia). Looking for the prevalence of this disease and the caracterization of kidney´s affections, an anathomopathological study was carried and in the kidneys of 100 asintomatic rabbits just when slaughtered at the "Hacienda El Progreso" of the Universidad de Antioquia. The diagnostic was based an the macroscopic find-out of the kidney lesions; Histhopathology by using the standard coloration Hematoxilina- Eosina and the parasite evidence by means of special coloration, Sandiford, ZiehlNeelsen and Warthin- Starry, Statistics analyses was realised by using descriptive statistic and independence and contingency of Chi square to stablish the association level between some variables under the study. Encephalitozoonosis prevalence in rabitts was 5% and it was allways in relation with interstitial nefritis , since, it can be concluded that this disease has a high rat of subclinical presentation and thus that, in the province of Antioquia there is a new emmerging disease which affects the rabbit industry and represent a public health patiental risk factor. Key words: Encephalitozoon cuniculi, interstitial nefritis, kidney, rabbit.
Hidrocefalia canina: Reporte de casos
Authors: Orozco P. Sonia C. , Aranzazu Diego 
Published: August 2024

Resumen La hidrocefalia es un agrandamiento del sistema ventricular cerebral secundario a un aumento en la cantidad de líquido cefalorraquídeo (LCR). Esta enfermedad puede ser de carácter adquirido o congénito. La signología que presentan los pacientes afectados, puede ir desde la ausencia de manifestación clínica hasta la presentación de alteraciones en el estado de conciencia. El tratamiento médico y/o quirúrgico puede llegar a mantener estable a estos pacientes, teniendo estos un pronóstico de reservado a malo. Este artículo presenta una revisión sobre aspectos de la fisiopatología, diagnóstico y tratamiento de esta entidad; y tres casos clínicos llegados al Consultorio Veterinario de la Universidad de Antioquia, los cuales presentaron como diagnóstico final Hidrocefalia congénita, cada uno presentando signología variada. Palabras clave: caninos, hidrocefalia, líquido cefalorraquídeo, presión intracraneal, trastornos del sistema nervioso, ventrículos. Summary Hydrocephalus is a pathologic enlargement of the ventricular system secondary to an increased volume of cerebrospinal fluid (CSF). This disease can be acquired or congenital. The clinical signs vary from total absence of manifestation to alteration of mental status. The medical and/or treatments could stabilize these patients, but their prognosis continuous to be guarded to poor. This article presents an overhaul of aspects of the pathophysiology, diagnostic plan and treatment of congenital hydrocephalus and three clinical cases that arrived the Veterinary Teaching Clinic of University of Antioquia, which presented as a definitive diagnose congenital hydrocephalus, each one with a myriad of clinical signs. Key words: acquired, cerebrospinal fluid, congenital, intracraneal pressure, ventricles.
Predicción del peso vivo a través del perímetro torácico en la raza bovina Lucerna
Authors: Mahecha Liliana , Angulo Joaquín , Manrique Luis P. 
Published: August 2024

Resumen Con base en el perímetro torácico y por barimetría se predijo el peso vivo de la raza Lucerna, utilizando 1273 animales de ambos sexos, con edades de un día a ocho años. Se realizó análisis de regresión con el modelo matemático Gamma (Y= AeBX Xc + Ei), con el cual se obtuvo la ecuación de predicción del peso vivo para esta raza. Una prueba de bondad de ajuste determinó la confiabilidad de los valores estimados y los resultados, establecieron que no hubo diferencia significativa y que hubo concordancia entre los pesos observados y los estimados para la elaboración de la tabla predictiva del peso vivo en la raza Lucerna. Palabras clave: barimetría, medidas bovinométricas, modelo gamma, tabla de predicción. Summary Prediction of the body weight throught the chest girth on the Lucerna breed The objetive was to predict the body weight throught barimetry based on the chest girth by using measurments of 1273 animals of both sex with ages between one day and eigth years of the Lucerna breed. Regression analysis was made with the Gamma model (Y= AeBX Xc + Ei) and a equation was obtained to predict the body weight of this breed. Was realized a proof in order to demostrate the confiability of the predicted data. Results demostrated that there wasn't significant differences between the body weight measured and the body weight predicted with the equation. Key words: barimetry, bovinometry, gamma model, measurements, table of prediction.
Respuesta inmune y estrategias de evasión durante la infección con Brucella spp.
Authors: Saldarriaga Omar A. , Ossa Londoño Jorge , Rugeles Lopez María Teresa 
Published: August 2024

Resumen La Brucella es una bacteria Gram-negativa, intracelular facultativa que infecta en forma persistente a humanos y animales domésticos y salvajes. Este género de bacterias se replica en los fagocitos mononucleares del hospedero, evadiendo los mecanismos solubles extracelulares de la respuesta inmune. La patogénesis de la infección por Brucella es bastante compleja, depende del patógeno y de los mecanismos de defensa que se activan, donde la inmunidad celular, macrófagos, citoquinas tipo Th1 y células citotóxicas participan activamente en la resolución de la infección. Aunque esta bacteria no evade los mecanismos de fagocitosis, si ha desarrollado mecanismos para la sobrevivencia intracelular. El entendimiento de la interrelación de la respuesta inmune con estos patógenos microbianos, permitirá el desarrollo de medidas para la prevención y el control. Esta revisión describe algunos de los mecanismos inmunológicos innatos y específicos involucrados en la eliminación de la infección causada por las especies de Brucella y referencia artículos originales que dan cuenta del avance logrado mediante la utilización de diferentes modelos animales. Palabras clave: B. abortus, inmunidad, sobrevivencia. Summary Brucella is a Gram-negative intracellular facultative bacterium causing persistent infection in humans and domestic and wild animals. This bacterial genus replicates in host mononuclear phagocytes evading the extracellular soluble mechanisms of the immune response. The infection induced by this bacterium is quite complex and depends on the pathogen and on the activated defense mechanisms of the host. Macrophages, Th 1 cytokines and citotoxic cells actively participate in the resolution of the infection. Although the macrophages of the host actively and efficiently phagocyte these bacteria, the parasite has developed mechanisms for intracellular survival. The understanding of the interaction of the immune system with these microbial pathogens would allow us the development of new efficient strategies directed to prevent and control this broadly distributed pathogen. This review emphasizes on some of the innate and specific immunologic mechanisms involved in the clearance of the infection caused by Brucella species and on recent literature on animal models of the disease. Key words: B. abortus, immunity, survival.
Detección y aislamiento del Parvovirus porcino en Medellín, Colombia
Authors: Rico Silvia , Molina Sadoh , Pabón Francisco 
Published: August 2024

Resumen Con el objetivo de detectar y aislar el Parvovirus porcino (PVP), se analizó el tejido pulmonar de 75 fetos porcinos momificados, procedentes de granjas de Medellín, por la técnica de Hemaglutinación (HA) con glóbulos rojos de cobayo y la propagación del virus en cultivos de la línea celular PK15 de riñón porcino. Los fetos presentaron un tamaño entre 8 y 25 cm. El 73.3% (55/75) de los fetos resultó positivo y 26.7% (20/75) negativo a la HA. El 93.54% (52/55) de los fetos positivos eran de 45 a 65 días de gestación y el 6.46% (3/55) eran de 66 a 75 días de edad; en cuanto a los casos negativos el 90%(18/20) era mayor de 76 días y 10% (2/20) de 66 a 75 días de edad. El aislamiento viral fue posible a partir de dos de los fetos positivos a HA 3.6% (2/55) de una edad estimada de 48 y 51 días respectivamente. Luego de su propagación en la línea celular PK15, se determinó un incremento en el título del virus medido por HA y su resistencia al tratamiento con éter al 20%, así como su estabilidad a pH 3.0 a 9.0 y formación de cuerpos de inclusión intranucleares. Adicionalmente, se concluyó que el PVP afectó un alto porcentaje de los fetos analizados y que el diagnóstico por HA de la infección con el virus presentó mayores títulos del antígeno viral en fetos de menos de 65 días de edad (17 cm de tamaño). Palabras clave: cerdos, cultivo celular, diagnóstico, hemaglutinación, reproducción, virus. Summary The objective of this study was to detect the viral antigen and isolate the porcine parvovirus (PVP). Supernatants of lung tissue of 75 dead porcine foetuses from selected farms in Colombia were studied by Haemagglutination assay (HA) and viral propagation in PK15 cell culture (Porcine Kidney). The foetuses had a crown rump length between 8 to 25cm. 73.3% (55/75) of the foetuses were positive to HA and the 93.54% (52/55) of the positive cases were 45 to 65 days old while the 6.46% (3/55) were 66 to 75 days old. The 26.7% (20/75) of the cases were negative to HA. The 90% (18/20) of the negative cases were 66 to 95 days old. The virus was isolated from two mummies positives to HA, 3.6% (2/55 ), which were 48 and 51 days old, respectively. It was characterized like PVP by its HA activity, cytopathic effect in PK15 cell culture, and it was resistant to 20% ether and stable at p H 3.0. The conclusion was that a high percentage of analysed foetuses were infected with PVP, and the detection of virus antigen by HA, presents higher titles in foetuses younger than 65 days (17 cm length). Key words: cell culture, diagnostic, haemagglutination, pigs, reproduction, virus.
Osteosarcoma generalizado y megaesófago secundario, ocasionados por infección de Spirocerca lupi en un canino
Authors: Londoño N. Yesid , Carmona R. Jorge Uriel , Giraldo M. Carlos Eduardo 
Published: August 2024

Resumen En este artículo se describe un caso clínico poco frecuente de osteosarcoma generalizado acompañado de megaesófago secundario adquirido, ocasionados por un granuloma parasitario torácico de Spirocerca lupi, en un canino bóxer adulto. Se presentan los hallazgos radiológicos y anatomo-patológicos y se discuten con la literatura revisada. Palabras clave: canino, megaesófago, osteosarcoma, Spirocerca lupi. Summary A rare clinical case of a generalized osteosarcoma with a secondary acquired megaesophagus, due to thoracic Spirocerca lupi granuloma in an adult boxer canine, is described in this paper. Both radiological and anatomo-pathological findings are presented and discussed with literature reviewed. Key words: canine, osteosarcoma, megaesophagus, Spirocerca lupi.
Simulación del metabolismo del nitrógeno en vacas lactantes
Authors: Correa Héctor J. 
Published: August 2024

Resumen Con la finalidad de simular algunos parámetros del metabolismo del nitrógeno en vacas lactantes se revisaron trabajos de investigación en los que aparecen publicados datos de las variables dependientes e independientes asumidas para el modelo. Se consideraron como variables independientes aquellas que pudiesen ser obtenidas en campo sin que exigiesen metodologías costosas y complicadas: producción de leche (PL), proteína cruda en la dieta (PCd), proteína cruda en la leche (PCl) y nitrógeno ureico en leche (NUL). Las dependientes fueron: Eficiencia en el uso del nitrógeno dietario para la producción de proteínas lácteas (Efic), proteína verdadera en leche (Pverd), nitrógeno no proteico en leche (NNPl), nitrógeno absorbido (Nabs), nitrógeno retenido (Nret), y nitrógeno urinario (Nuri). Aritméticamente y teniendo en cuenta el contenido de materia seca y proteína cruda tanto del forraje como del suplemento concentrado, se calculó el nitrógeno excretado en heces (Nexcr), el nitrógeno consumido (Ncons), la materia seca consumida total (MSCtotal), la materia seca consumida desde el suplemento (MSconc), la materia seca consumida desde el forraje (MSforr), y la materia verde consumida del forraje (MVforr). Para este fin se utilizaron 29 datos provenientes de seis trabajos publicados en revistas científicas de amplia circulación. Se analizaron ecuaciones de predicción lineal múltiple con el programa SAS para Efic, Pverd, Nabs, Nret y Nuri. Palabras clave: balance de nitrógeno, eficiencia nutricional, modelos matemáticos. Summary To simulate some parameters of the nitrogen metabolism in lactating cows, some research articles about the independent and dependent variables included in the assumed model, were reviewed. Independent variables that can be measured in situ without expensive and complicated diagnosis methods were considered: milk yield (PL), diet crude protein (PCd), milk crude protein (PCl) and milk ureic nitrogen (NUL). The dependent variables were: efficiency of nitrogen utilization to milk protein production (Efic), milk true protein (Pverd), milk non protein nitrogen (NNPl), absorbed nitrogen (Nabs), retained nitrogen (Nret), and urinary nitrogen (Nuri). Based on dry matter and crude protein contained in forage and also in concentrate supplement, the nitrogen excreted in feces (Nexcr), the nitrogen intake (Ncons), the total dry matter intake (MSCtotal), the dry matter intake from supplement (MScons), the dry matter intake from forage (MSforr), and the wet matter intake from forage (MVforr) were arithmetically calculated. To obtain this results, 29 values from six research articles published in scientific journals were used and lineal multiple equations were analysed with SAS software to Efic, Pverd, Nabs, Nret and Nuri. Key words: mathematic models, nitrogen balance, nutritional efficiency.
Study on University Dropouts In the Context of the Romanian Education System Reform
Authors: Călin Mariana Floricica , Flaut Cristina , Flaut Daniel , Tușa Enache 
Published: August 2024

After 2010, the Romanian university system registered an increase of dropout cases. This aspect requires to identify and implement some alternative methods of action at the national level with the unique scope to prevent and combat early university dropout. The good motivation of the students for their studies is increasingly low. Some of the causes which determine this phenomenon are well known and quite difficult to remove, as for example the financial and intellectual development level of the family from where they come. These aspects can often be decisive factors in the emergence of the school dropout phenomenon. But these aspects are certainly not the only causes. This is the reason for which other aspects, which determine the students to dropping out the school and can be more easily corrected, must be identified. The aim of this paper is to present these aspects, emphasizing the causes, consequences, and potential solutions to limit absenteeism and university dropout. For this purpose, we present a questionnaire applied at "Ovidius" University on a target group of students. The participants consisted of 500 students from 5 faculties of our university. With this questionnaire we want to identify patterns and trends in the evolution of absenteeism and university dropout.
Digital Technology in The Training of Future Kinesiotherapists
Authors: Cojocaru Aurora Liliana , Timnea Olivia Carmen 
Published: August 2024

Digital technology has permeated all fields of activity, therefore it is absolutely necessary to use it in the education system as well. Today`s students should benefit from the conveniences offered by the digital world; the information activity must thus be permanently adapted to the digital evolution, so that the future specialists can face the demands of society. Kinesiotherapy and Special Motricity students, future kinesiotherapists, need solid knowledge of human anatomy, this knowledge being a support for their whole activity. Digital technology can supplement the traditional way of learning human anatomy. Various apps have been designed for learning human anatomy, among them Complete Anatomy. Created in 2009 by 3D4Medical, now part of Elsevier, Complete Anatomy is an application that offers an alternative to physical anatomical models, through interactive anatomical models; the application can be installed on various devices: laptop, tablet, smartphone and can be accessed anytime and anywhere the student is, both during class/lab and outside them, for individual study. Using the Complete Anatomy app, muscle actions can be visualized; movement analysis being important in the training of future kinesiotherapists. In addition to detailed 3D anatomical models, the app also provides anatomical information and a video library. For the research, the group of subjects was made up of first-year students from the Faculty of Physical Education, Sport and Kinesiotherapy, specialization in Kinesiotherapy and Special Motricity, Romanian-American University of Bucharest. Following the use of a questionnaire, it was found that, regardless of the experience of previous use or not of some applications in learning, students are eager to use digital technology for learning human anatomy through the Complete Anatomy application.
Four Azadirone-Type Limonoids from Chisocheton Pentandrus Stem Bark and Their Cytotoxic Acitivity Against Mcf-7 Breast Cancer Cell Lines
Authors: Supratman Unang , Runadi Dudi , Naini Al Arofatus , Purnama Purnama , Mayanti Tri , Harneti Desi , Nurlelasari Nurlelasari , Maharani Rani , Farabi Kindi , Nafiah Mohamad Azlan 
Published: August 2024

ABSTRACT. Limonoid group belongs to triterpenoid that has undergone further oxidation accompanied by the loss of four carbon atoms to form a furan ring in the chain. This limonoid compound is often found in the Chisocheton genus. Limonoid compounds have been known as compounds with high structural variations and this makes limonoids have diverse and interesting activities, including cytotoxic and anticancer. In the course of our continuing study for limonoid constituents that have cytotoxic activity against cancer cell lines, methanol extract of stem bark from the Chisocheton pentandrus plant provides significant activity. The methanol extract was separated using various chromatographic techniques in the normal and reverse stationary phase to produce four azadirone-type limonoid compounds (1-4). The elucidation structure of 1-4 was determined using spectroscopic methods including, UV-Visible, IR, and 1D-NMR as well as optical rotation. All four known compounds were established as trichilenone acetate (1), toonaciliaton C (2), 11α-acetoxiazadiron (3), and 16β-hydroxydisobinine (4). The cytotoxicity of compounds 1-4 was assessed by examination using the resazurin method, which showed that compound 4 was the promising constituent against the MCF-7 cells with an IC50 value of 43.1 µM and was stronger than its positive control.

Keywords: Azadirone-type limonoid, Chisocheton pentandrus, cytotoxic activity, Meliaceae.
Cationic Dye Adsorption Using Silica Xerogel from Corn Husk in A Continuous Fixed-Bed Column
Authors: Dahliyanti Ayu , Nabila Luthfia Jihan , Pratama Arka Krisna , Widharyanti Ika Dyah 
Published: August 2024

ABSTRACT. Silica xerogel from corn husks shows potential to be applied as an adsorbent for cationic dye removal due to its high adsorption capacity. However, the study regarding its performance in a continuous operation is still very limited, which hinders its application as a commercial adsorbent. To answer that problem, we investigate the adsorption of methylene blue (MB) and crystal violet (CV) dye using silica xerogel from corn husk in a continuous fixed-bed column. A breakthrough curve analysis was carried out with flow rate and bed height variations in an up-flow mode. The column effluent concentration was analyzed using a UV-Vis Spectrophotometer. The experimental data shows good agreement with the Thomas/Bohart-Adams and Yoon-Nelson model at different flow rates but has less correspondence for increasing bed height. Maximum adsorption capacities for methylene blue were 1.977 mg/g (Thomas/BA) and 1.743 mg/g (experimental). While for crystal violet the maximum adsorption capacities were 3.400 mg/g (Thomas/BA) and 4.573 mg/g (experimental).

Keywords: adsorption, cationic dyes, silica xerogel, fixed-bed column, mathematical model.