bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of a Hypoglycemia Treatment Protocol on Glycemic Variability in Critically Ill Patients
Authors: Arnold Patrick , Paxton Renee Alexander , McNorton Kelly , Szpunar Susan , Edwin Stephanie B. 
Published: November 2013

Introduction: Hypoglycemia and glucose variability are independently associated with increased mortality in septic, surgical, and mixed intensive care unit (ICU) patients. Treatment of hypoglycemia with dextrose 50% can overcorrect blood glucose levels and increase glucose variability. The purpose of this study is to evaluate the effect of a hypoglycemia treatment protocol focused on minimizing glucose variability in critically ill patients. Methods: This retrospective analysis was conducted at a 772-bed community teaching hospital in Detroit, Michigan. A standardized nursing-driven hypoglycemia treatment protocol specific to critically ill patients was implemented. Glucose variability, amount of dextrose administered, subsequent glucose monitoring, hypoglycemia recurrence, and mortality were compared between pre- and postprotocol groups. Results: The coefficient of variability of blood glucose in the postprotocol group (n = 53) was decreased compared with the preprotocol group (n = 52), 40.9% versus 49.3%, respectively ( P = .048). Dextrose usage was significantly reduced between groups (21.2 g preprotocol vs 11.5 g postprotocol; P < .001). The time to first blood glucose check was 36 minutes after protocol implementation compared to 61 minutes before the protocol ( P = .003). Finally, the incidence of continued hypoglycemia following dextrose administration and ICU mortality was similar between groups. Conclusions: Implementation of the hypoglycemia treatment protocol described led to a reduction in glucose variability, while still providing a safe and effective way to manage hypoglycemia in critically ill patients.
Application of ultrasound-assisted emulsification microextraction for simultaneous determination of aminophenol isomers in human urine, hair dye, and water samples using high-performance liquid chromatography
Authors: Asghari Alireza , Fazl-Karimi Hamidreza , Barfi Behruz , Rajabi Maryam , Daneshfar Ali 
Published: November 2013

Aminophenol isomers (2-, 3-, and 4-aminophenols) are typically classified as industrial pollutants with genotoxic and mutagenic effects due to their easy penetration through the skin and membranes of human, animals, and plants. In the present study, a simple and efficient ultrasound-assisted emulsification microextraction procedure coupled with high-performance liquid chromatography with ultraviolet detector was developed for preconcentration and determination of these compounds in human fluid and environmental water samples. Effective parameters (such as type and volume of extraction solvent, pH and ionic strength of sample, and ultrasonication and centrifuging time) were investigated and optimized. Under optimum conditions (including sample volume: 5 mL; extraction solvent: chloroform, 80 µL; pH: 6.5; without salt addition; ultrasonication: 3.5 min; and centrifuging time: 3 min, 5000 rpm min−1), the enrichment factors and limits of detection were ranged from 42 to 51 and 0.028 to 0.112 µg mL−1, respectively. Once optimized, analytical performance of the method was studied in terms of linearity (0.085–157 µg mL−1, r2 > 0.998), accuracy (recovery = 88.6– 101.7%), and precision (repeatability: intraday precision < 3.98%, and interday precision < 5.12%). Finally, applicability of the method was evaluated by the extraction and determination of these compounds in human urine, hair dye, and real water samples.
Cytotoxicity evaluation of dentin contacting materials with dentin barrier test device using erbium-doped yttrium, aluminum, and garnet laser-treated dentin
Authors: Ülker HE , Ülker M , Botsalı MS , Dündar A , Acar H 
Published: November 2013

Objectives: The effect of dentin contacting materials on three-dimensional cultures of pulp-derived cells was evaluated in a dentin barrier test device using erbium-doped yttrium, aluminum, and garnet (Er:YAG) laser-treated dentin. Methods: The test materials (iBond®, G-Bond™, and Vitrebond™) were applied on laser-treated or untreated dentin discs. After 24 h of exposure with perfusion of the test chamber, cell survival was evaluated by enzyme activity and related to a nontoxic control material. The mean values of control tissues were set to represent 100% viability. Data were analyzed using Kruskal–Wallis and Mann–Whitney U test. Results: Vitrebond was the most toxic material for both laser-treated and untreated dentin. On untreated dentin, G-bond was cytotoxic to the pulp-derived cells ( p < 0.05), and iBond was similar to the negative control group ( p > 0.05). However, G-Bond and iBond were not cytotoxic when they were applied to Er:YAG laser-treated dentin ( p > 0.05). Conclusion: Er:YAG laser treatment of dentin may protect the pulp cells from toxic substances of dentin contacting restorative materials; however, this effect is material related. Taking into consideration the limitations of this in vitro study, the Er:YAG laser treatment of dentin before restoration might be an option for decreasing the cytotoxic effects of the dental materials. Further research is required for clinical applications.
Risk Factors Affecting Chronic Rupture of the Plantar Fascia
Authors: Lee Ho Seong , Choi Young Rak , Kim Sang Woo , Lee Jin Yong , Seo Jeong Ho , Jeong Jae Jung 
Published: November 2013

Background: Prior to 1994, plantar fascia ruptures were considered as an acute injury that occurred primarily in athletes. However, plantar fascia ruptures have recently been reported in the setting of preexisting plantar fasciitis. We analyzed risk factors causing plantar fascia rupture in the presence of preexisting plantar fasciitis. Methods: We retrospectively reviewed 286 patients with plantar fasciitis who were referred from private clinics between March 2004 and February 2008. Patients were divided into those with or without a plantar fascia rupture. There were 35 patients in the rupture group and 251 in the nonrupture group. The clinical characteristics and risk factors for plantar fascia rupture were compared between the 2 groups. We compared age, gender, the affected site, visual analog scale pain score, previous treatment regimen, body mass index, degree of ankle dorsiflexion, the use of steroid injections, the extent of activity, calcaneal pitch angle, the presence of a calcaneal spur, and heel alignment between the 2 groups. Results: Of the assessed risk factors, only steroid injection was associated with the occurrence of a plantar fascia rupture. Among the 35 patients with a rupture, 33 had received steroid injections. The odds ratio of steroid injection was 33. Conclusion: Steroid injections for plantar fasciitis should be cautiously administered because of the higher risk for plantar fascia rupture. Level of Evidence: Level III, retrospective comparative study.
Heme triggers TLR4 signaling leading to endothelial cell activation and vaso-occlusion in murine sickle cell disease
Authors: Belcher John D. , Chen Chunsheng , Nguyen Julia , Milbauer Liming , Abdulla Fuad , Alayash Abdu I. , Smith Ann , Nath Karl A. , Hebbel Robert P. , Vercellotti Gregory M. 
Published: November 2013

Key PointsHeme, released from hemoglobin, elicits vaso-occlusion in transgenic sickle mice via endothelial TLR4 signaling. Heme/TLR4 signaling activates NF-κB and triggers vaso-occlusion through Weibel-Palade body degranulation and adhesion molecule expression.
Aggf1 acts at the top of the genetic regulatory hierarchy in specification of hemangioblasts in zebrafish
Authors: Li Lei , Chen Di , Li Jia , Wang Xiaojing , Wang Nan , Xu Chengqi , Wang Qing K. 
Published: November 2013

Key PointsAggf1 is required for both primitive and definitive hematopoiesis. Aggf1 is the earliest known regulator for differentiation of hemangioblasts.
UKALLXII/ECOG2993: addition of imatinib to a standard treatment regimen enhances long-term outcomes in Philadelphia positive acute lymphoblastic leukemia
Authors: Fielding Adele K. , Rowe Jacob M. , Buck Georgina , Foroni Letizia , Gerrard Gareth , Litzow Mark R. , Lazarus Hillard , Luger Selina M. , Marks David I. , McMillan Andrew K. , Moorman Anthony V. , Patel Bella , Paietta Elisabeth , Tallman Martin S. , Goldstone Anthony H. 
Published: November 2013

Key Points
Imatinib improves outcomes for adults with Ph+ ALL at least in part by facilitating allogeneic stem cell transplant. Allogeneic hematopoietic stem cell transplant is not dispensible in Ph+ ALL in the imatinib era.
Injudicious use of laboratory facilities in tertiary care hospitals at rawalpindi, pakistan: a cross-sectional descriptive study
Authors: Malik Muhammad Farooq , Khan Dilshad Ahmed , Ansari Wafa Munir , Khan Farooq Ahmad 
Published: November 2013

Abstract

Background
In recent years inappropriate and excessive use of clinical laboratory facilities has become a cause of concern and has led to concurrent rise in the laboratory errors and the health care costs. The aim of the study was to find out the frequency of incomplete laboratory request forms, inappropriate test requests at various professional levels and the financial impact of uncollected reports at Armed Forces Institute of Pathology (AFIP) and Combined Military Hospital (CMH) Laboratory Rawalpindi.

Methods
The cross-sectional descriptive study was conducted during a three month period from April to June 2012 at AFIP and CMH Laboratory Rawalpindi. A total of 1000 laboratory request forms were collected and scrutinized for completion from AFIP (n=500) and CMH Rawalpindi laboratory (n=500). 536 request forms of costly/specialized tests from different departments of AFIP were studied to find out the professional level of test request. The total number of tests performed at AFIP during the study period and number of uncollected reports were noted. The financial impact of these uncollected reports was also calculated. Collection of data and sorting were done manually. Patient confidentiality was maintained. Microsoft excel software and SPSS-17 were used for analysis. The study was approved by the Institutional Ethical Review Committee.

Results
Out of a total of 1000 forms studied none was completely filled with clinical notes being present in only 2.4% and 13% of forms sent to CMH and AFIP respectively. 62% of the expensive investigations were requested by specialists while 38% were ordered by residents and general practitioners but the percentage of avoidable expensive tests ordered by the general practitioners and residents was significantly higher than the specialists(p<0.001). A total of 9026 (40%) and 5046 (22%) diagnostic test reports were not collected from the Chemical pathology and Hematology departments respectively. Financial impact of uncollected reports from all the departments at AFIP collectively amounted to Pakistani Rupees (PKR) 3338201.

Conclusion
Processing incomplete laboratory request forms and injudicious use of laboratory facilities leads to incorrect interpretation of laboratory test results affecting outcome of the overall treatment.
Genetic mutations in influenza H3N2 viruses from a 2012 epidemic in Southern China
Authors: Zhong Jing , Liang Lijun , Huang Ping , Zhu Xiaolan , Zou Lirong , Yu Shouyi , Zhang Xin , Zhang Yonghui , Ni Hanzhong , Yan Jin 
Published: November 2013

Abstract

Background
An influenza H3N2 epidemic occurred throughout Southern China in 2012.

Methods
We analyzed the hemagglutinin (HA) and neuraminidase (NA) genes of influenza H3N2 strains isolated between 2011–2012 from Guangdong. Mutation sites, evolutionary selection, antigenic sites, and N-glycosylation within these strains were analyzed.

Results
The 2011–2012 Guangdong strains contained the HA-A214S, HA-V239I, HA-N328S, NA-L81P, and NA-D93G mutations, similar to those seen in the A/ Perth/16/2009 influenza strain. The HA-NSS061–063 and NNS160–162 glycosylation sites were prevalent among the 2011–2012 Guangdong strains but the NA-NRS402–404 site was deleted. Antigenically, there was a four-fold difference between A/Perth/16/2009 -like strains and the 2011–2012 Guangdong strains.

Conclusion
Antigenic drift of the H3N2 subtype contributed to the occurrence of the Southern China influenza epidemic of 2012.
Brucellosis seroprevalence in Bali cattle with reproductive failure in South Sulawesi and Brucella abortus biovar 1 genotypes in the Eastern Indonesian archipelago
Authors: Muflihanah Hanah , Hatta Mochammad , Rood Ente , Scheelbeek Pauline , Abdoel Theresia H , Smits Henk L 
Published: November 2013

Abstract

Background
Brucellosis is a major cause of infertility and reproductive failure in livestock. While cattle in the Eastern Indonesian archipelago suffers from reproductive problems information on bovine brucellosis in the region is fragmentary. The control of brucellosis requires a major and prolonged effort and confirmation of the infection by isolation with detailed knowledge of the spread of the infection is essential when planning a control program.

Results
Serological investigation of Brucella infection in beef cattle tended under extensive farming conditions revealed a high seroprevalence (19.3%; 95% CI, 17–22) in the compliment fixation tests. The results of a rapid and simple field test correlated well with the Rose Bengal test (kappa, 0.917) and indicated an acceptable sensitivity (87.5%) and specificity (98.1%) compared with the complement fixation test. Reproductive failure was reported for 39.0% of the cows with a loss of calves due to abortion or early death amounting to 19.3%. Past reproductive failure did not, however, correlate with seropositivity in the complement fixation test (RP = 1.21; P = 0.847). B. abortus biovar 1 was freshly isolated from the hygromas of two cows and together with thirty banked isolates collected since 1990 from different parts of Sulawesi and Timor eight related genotypes could be distinguished with one genotype being identical to that of an isolate (BfR91) from Switzerland. The Indonesian genotypes formed together with BfR91 and one African and one North American isolate a distinct branch on the B. abortus biovar 1 dendogram.

Conclusions
Bovine brucellosis appears to be widespread in the Eastern Indonesian archipelago and calls for urgent intervention. The fresh isolation of the pathogen together with the observed high seroprevalence demonstrates the presence and frequent exposure of cattle in the area to the pathogen. The application of a rapid and simple field test for brucellosis could be very useful for the quick screening of cattle at the pen side.
Antioxidant activity of herbaceous plant extracts protect against hydrogenperoxide-induced DNA damage in human lymphocytes
Authors: Lin Kuan-Hung , Yang Yan-Yin , Yang Chi-Ming , Huang Meng-Yuan , Lo Hsiao-Feng , Liu Kuang-Chuan , Lin Hwei-Shen , Chao Pi-Yu 
Published: November 2013

Abstract

Background
Herbaceous plants containing antioxidants can protect against DNA damage. Thepurpose of this study was to evaluate the antioxidant substances,antioxidant activity, and protection of DNA from oxidative damage in humanlymphocytes induced by hydrogen peroxide (H2O2). Ourmethods used acidic methanol and water extractions from six herbaceousplants, including Bidens alba (BA), Lycium chinense (LC),Mentha arvensis (MA), Plantago asiatica (PA),Houttuynia cordata (HC), and Centella asiatica(CA).

Methods
Antioxidant compounds such as flavonol and polyphenol were analyzed.Antioxidant activity was determined by the inhibition percentage ofconjugated diene formation in a linoleic acid emulsion system and bytrolox-equivalent antioxidant capacity (TEAC) assay. Their antioxidativecapacities for protecting human lymphocyte DNA fromH2O2-induced strand breaks was evaluated by cometassay.

Results
The studied plants were found to be rich in flavonols, especially myricetinin BA, morin in MA, quercetin in HC, and kaemperol in CA. In addition,polyphenol abounded in BA and CA. The best conjugated diene formationinhibition percentage was found in the acidic methanolic extract of PA.Regarding TEAC, the best antioxidant activity was generated from the acidicmethanolic extract of HC. Water and acidic methanolic extracts of MA and HCboth had better inhibition percentages of tail DNA% and tail moment ascompared to the rest of the tested extracts, and significantly suppressedoxidative damage to lymphocyte DNA.

Conclusion
Quercetin and morin are important for preventing peroxidation and oxidativedamage to DNA, and the leaves of MA and HC extracts may have excellentpotential as functional ingredients representing potential sources ofnatural antioxidants.
Neurotology symptoms at referral to vestibular evaluation
Authors: Jáuregui-Renaud Kathrine , Gutierrez-Marquez Aralia , Viveros-Rentería Leticia , Ramos-Toledo Verónica , Gómez-Alvarez Fátima 
Published: November 2013

Background Dizziness-vertigo is common in adults, but clinical providers may rarely diagnose vestibular impairment and referral could be delayed. To assess neurotology symptoms (including triggers) reported by patients with peripheral vestibular disease, during the year just before their referral to vestibular evaluation. Methods 282 patients with peripheral vestibular disease and 282 control subjects accepted to participate. They had no middle ear, retinal, neurological, psychiatric, autoimmune or autonomic disorders. They reported their symptoms by a standardized questionnaire along with their anxiety/depression symptoms. Results Patients were referred after months or years from the onset of their symptoms, 24% of them reported frequent falls with a long clinical evolution; 10% of them reported no vertigo but instability related to specific triggers; 86% patients and 12% control subjects reported instability when moving the head rapidly and 79% patients and 6% control subjects reported instability when changing posture. Seven out of the 9 symptoms explored by the questionnaire allowed the correct classification of circa 95% of the participants (Discriminant function analysis, p < 0.001). High blood pressure, dyslipidemia and anxiety/depression symptoms showed a mild correlation with the total score of symptoms (multiple R2 =0.18, p < 0.001). Conclusions Late referral to vestibular evaluation may underlie a history of frequent falls; some patients may not report vertigo, but instability related to specific triggers, which could be useful to prompt vestibular evaluation. High blood pressure, dyslipidemia and anxiety/depression symptoms may have a mild influence on the report of symptoms of vestibular disease in both, patients and control subjects.
Introducing glycomics data into the Semantic Web
Authors: Aoki-Kinoshita Kiyoko F , Bolleman Jerven , Campbell Matthew P , Kawano Shin , Kim Jin-Dong , Lütteke Thomas , Matsubara Masaaki , Okuda Shujiro , Ranzinger Rene , Sawaki Hiromichi , Shikanai Toshihide , Shinmachi Daisuke , Suzuki Yoshinori , Toukach Philip , Yamada Issaku , Packer Nicolle H , Narimatsu Hisashi 
Published: November 2013

Abstract

Background
Glycoscience is a research field focusing on complex carbohydrates (otherwise known as glycans)a, which can, for example, serve as “switches” that toggle between different functions of a glycoprotein or glycolipid. Due to the advancement of glycomics technologies that are used to characterize glycan structures, many glycomics databases are now publicly available and provide useful information for glycoscience research. However, these databases have almost no link to other life science databases.

Results
In order to implement support for the Semantic Web most efficiently for glycomics research, the developers of major glycomics databases agreed on a minimal standard for representing glycan structure and annotation information using RDF (Resource Description Framework). Moreover, all of the participants implemented this standard prototype and generated preliminary RDF versions of their data. To test the utility of the converted data, all of the data sets were uploaded into a Virtuoso triple store, and several SPARQL queries were tested as “proofs-of-concept” to illustrate the utility of the Semantic Web in querying across databases which were originally difficult to implement.

Conclusions
We were able to successfully retrieve information by linking UniCarbKB, GlycomeDB and JCGGDB in a single SPARQL query to obtain our target information. We also tested queries linking UniProt with GlycoEpitope as well as lectin data with GlycomeDB through PDB. As a result, we have been able to link proteomics data with glycomics data through the implementation of Semantic Web technologies, allowing for more flexible queries across these domains.
The Ontology of Vaccine Adverse Events (OVAE) and its usage in representing and analyzing adverse events associated with US-licensed human vaccines
Authors: Marcos Erica , Zhao Bin , He Yongqun 
Published: November 2013

Abstract

Background
Licensed human vaccines can induce various adverse events (AE) in vaccinated patients. Due to the involvement of the whole immune system and complex immunological reactions after vaccination, it is difficult to identify the relations among vaccines, adverse events, and human populations in different age groups. Many known vaccine adverse events (VAEs) have been recorded in the package inserts of US-licensed commercial vaccine products. To better represent and analyze VAEs, we developed the Ontology of Vaccine Adverse Events (OVAE) as an extension of the Ontology of Adverse Events (OAE) and the Vaccine Ontology (VO).

Results
Like OAE and VO, OVAE is aligned with the Basic Formal Ontology (BFO). The commercial vaccines and adverse events in OVAE are imported from VO and OAE, respectively. A new population term ‘human vaccinee population’ is generated and used to define VAE occurrence. An OVAE design pattern is developed to link vaccine, adverse event, vaccinee population, age range, and VAE occurrence. OVAE has been used to represent and classify the adverse events recorded in package insert documents of commercial vaccines licensed by the USA Food and Drug Administration (FDA). OVAE currently includes over 1,300 terms, including 87 distinct types of VAEs associated with 63 human vaccines licensed in the USA. For each vaccine, occurrence rates for every VAE in different age groups have been logically represented in OVAE. SPARQL scripts were developed to query and analyze the OVAE knowledge base data. To demonstrate the usage of OVAE, the top 10 vaccines accompanying with the highest numbers of VAEs and the top 10 VAEs most frequently observed among vaccines were identified and analyzed. Asserted and inferred ontology hierarchies classify VAEs in different levels of AE groups. Different VAE occurrences in different age groups were also analyzed.

Conclusions
The ontology-based data representation and integration using the FDA-approved information from the vaccine package insert documents enables the identification of adverse events from vaccination in relation to predefined parts of the population (age groups) and certain groups of vaccines. The resulting ontology-based VAE knowledge base classifies vaccine-specific VAEs and supports better VAE understanding and future rational AE prevention and treatment.