bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrogastrography measurement systems and analysis methods used in clinical practice and research: comprehensive review
Authors: Oczka David , Augustynek Martin , Penhaker Marek , Kubicek Jan 
Published: July 2024

Electrogastrography (EGG) is a non-invasive method with high diagnostic potential for the prevention of gastroenterological pathologies in clinical practice. In this study, a review of the measurement systems, procedures, and methods of analysis used in electrogastrography is presented. A critical review of historical and current literature is conducted, focusing on electrode placement, measurement apparatus, measurement procedures, and time-frequency domain methods of filtration and analysis of the non-invasively measured electrical activity of the stomach. As a result, 129 relevant articles with primary aim on experimental diet were reviewed in this study. Scopus, PubMed, and Web of Science databases were used to search for articles in English language, according to the specific query and using the PRISMA method. The research topic of electrogastrography has been continuously growing in popularity since the first measurement by professor Alvarez 100 years ago, and there are many researchers and companies interested in EGG nowadays. Measurement apparatus and procedures are still being developed in both commercial and research settings. There are plenty variable electrode layouts, ranging from minimal numbers of electrodes for ambulatory measurements to very high numbers of electrodes for spatial measurements. Most authors used in their research anatomically approximated layout with two++ active electrodes in bipolar connection and commercial electrogastrograph with sampling rate of 2 or 4 Hz. Test subjects were usually healthy adults and diet was controlled. However, evaluation methods are being developed at a slower pace, and usually the signals are classified only based on dominant frequency. The main review contributions include the overview of spectrum of measurement systems and procedures for electrogastrography developed by many authors, but a firm medical standard has not yet been defined. Therefore, it is not possible to use this method in clinical practice for objective diagnosis.Systematic Review Registrationhttps://www.prisma-statement.org/.
Sub-MIC antibiotics increased the fitness cost of CRISPR-Cas in Acinetobacter baumannii
Authors: Yu Ting , Huang Jiayuan , Huang Xinyue , Hao Jingchen , Zhang Pengyu , Guo Tingting , Bao Guangyu , Li Guocai 
Published: July 2024

IntroductionThe escalating prevalence of bacterial resistance, particularly multidrug-resistant bacteria like Acinetobacter baumannii, has become a significant global public health concern. The CRISPR-Cas system, a crucial defense mechanism in bacteria against foreign genetic elements, provides a competitive advantage. Type I-Fb and Type I-Fa are two subtypes of CRISPR-Cas systems that were found in A. baumannii, and the I-Fb CRISPR-Cas system regulates antibiotic resistance in A. baumannii. However, it is noteworthy that a majority of clinical isolates of A. baumannii lack or have incomplete CRISPR-Cas systems and most of them are multidrug-resistant. In light of this, our study aimed to examine the impact of antibiotic pressure on the fitness cost of the I-Fb CRISPR-Cas system in A. baumannii.Methods and ResultsIn the study, we conducted in vitro competition experiments to investigate the influence of sub-minimum inhibitory concentration (sub-MIC) on the CRISPR-Cas systems’ fitness cost in A. baumannii. We found that the fitness cost of the CRISPR-Cas system was increased under sub-MIC conditions. The expression of CRISPR-Cas-related genes was decreased, while the conjugation frequency was increased in AB43 under sub-MIC conditions. Through metabolomic analysis, we identified that sub-MIC conditions primarily affected energy metabolism pathways. In particular, we observed increased carbon metabolism, nitrogen metabolism, and intracellular ATP. Notably, the CRISPR-Cas system demonstrated resistance to the efflux pump-mediated resistance. Furthermore, the expression of efflux pump-related genes was increased under sub-MIC conditions.ConclusionOur findings suggest that the I-Fb CRISPR-Cas system confers a significant competitive advantage in A. baumanni. However, under sub-MIC conditions, its function and the ability to inhibit the energy required for efflux pumps are reduced, resulting in an increased fitness cost and loss of competitive advantage.
Effects of daily probiotic supplementation with Lactobacillus acidophilus on calcium status, bone metabolism biomarkers, and bone mineral density in postmenopausal women: a controlled and randomized clinical study
Authors: Harahap Iskandar Azmy , Moszak Małgorzata , Czlapka-Matyasik Magdalena , Skrypnik Katarzyna , Bogdański Paweł , Suliburska Joanna 
Published: July 2024

BackgroundMenopause poses significant health risks for women, particularly an increased vulnerability to fractures associated with osteoporosis. Dietary interventions have emerged as promising strategies, focusing on mitigating the risk of osteoporosis rather than solely addressing the established disease. This 12-week randomized controlled trial aimed to analyze the effects of consuming Lactobacillus acidophilus probiotics on calcium levels, biomarkers of bone metabolism, and bone mineral density (BMD) profiles in postmenopausal women.MethodsFifty-five participants were randomly assigned to receive either a placebo (n = 25) or the probiotic L. acidophilus UALa-01™ (n = 30) daily via oral intervention. Throughout the study, evaluations included body composition, blood biochemical parameters, serum calcium levels, and biomarkers of bone metabolism. Additionally, Dual-energy X-ray absorptiometry was used to measure BMD profiles.ResultsThe findings delineated that the probiotic group experienced a decrease in serum calcium levels compared to their initial levels. However, hair calcium levels and biomarkers related to bone metabolism showed no notable changes within this group. Consumption of probiotic L. acidophilus also seemed to prevent fluctuations in bone turnover markers. Moreover, there were no significant alterations in BMD levels at the lumbar spine, left femur, and total body in the probiotic group. Additionally, probiotic intake led to favorable outcomes by significantly reducing both body fat and visceral fat during the intervention period. Conversely, an adverse effect of consuming probiotic L. acidophilus was observed with a significant increase in glucose concentration.ConclusionIn conclusion, the consumption of L. acidophilus probiotics daily for 12 weeks among postmenopausal women does not affect the profile of BMD, but it may help in stabilizing bone turnover. It is important to note that most measured parameters were within the normal range for this population. However, it is worth noting that 3 months of probiotic supplementation could potentially disrupt calcium and glucose status in postmenopausal women.
Segmentation model of soft tissue sarcoma based on self-supervised learning
Authors: Zheng Minting , Guo Chenhua , Zhu Yifeng , Gang Xiaoming , Fu Chongyang , Wang Shaowu 
Published: July 2024

IntroductionSoft tissue sarcomas, similar in incidence to cervical and esophageal cancers, arise from various soft tissues like smooth muscle, fat, and fibrous tissue. Effective segmentation of sarcomas in imaging is crucial for accurate diagnosis.MethodsThis study collected multi-modal MRI images from 45 patients with thigh soft tissue sarcoma, totaling 8,640 images. These images were annotated by clinicians to delineate the sarcoma regions, creating a comprehensive dataset. We developed a novel segmentation model based on the UNet framework, enhanced with residual networks and attention mechanisms for improved modality-specific information extraction. Additionally, self-supervised learning strategies were employed to optimize feature extraction capabilities of the encoders.ResultsThe new model demonstrated superior segmentation performance when using multi-modal MRI images compared to single-modal inputs. The effectiveness of the model in utilizing the created dataset was validated through various experimental setups, confirming the enhanced ability to characterize tumor regions across different modalities.DiscussionThe integration of multi-modal MRI images and advanced machine learning techniques in our model significantly improves the segmentation of soft tissue sarcomas in thigh imaging. This advancement aids clinicians in better diagnosing and understanding the patient's condition, leveraging the strengths of different imaging modalities. Further studies could explore the application of these techniques to other types of soft tissue sarcomas and additional anatomical sites.
Induction chemotherapy with paclitaxel, carboplatin, and cetuximab (PCE) followed by chemoradiotherapy for unresectable locoregional recurrence after curative surgery in patients with squamous cell carcinoma of the head and neck
Authors: Sato Masanobu , Enokida Tomohiro , Fujisawa Takao , Okano Susumu , Takeshita Naohiro , Tanaka Nobukazu , Tanaka Hideki , Motegi Atsushi , Zenda Sadamoto , Shinozaki Takeshi , Matsuura Kazuto , Hayashi Ryuichi , Akimoto Tetsuo , Tahara Makoto 
Published: July 2024

BackgroundThe significance of induction chemotherapy (IC) in the treatment of squamous cell carcinoma of the head and neck (SCCHN) with unresectable locoregional recurrence after curative surgery has not been clarified. The aim of this study was to evaluate the efficacy of IC followed by chemoradiotherapy (CRT) in these patients.MethodsAmong patients with unresectable locoregional recurrent SCCHN who had not undergone prior irradiation and were eligible for cisplatin, we conducted a retrospective analysis of patients who received CRT following IC with paclitaxel, carboplatin, or cetuximab (IC-PCE group) and those who received CRT without prior IC (CRT group) between June 2013 and August 2021.ResultForty-two patients were included. The CRT group and IC-PCE group consisted of 15 and 27 patients, respectively. Primary site was the oral cavity (n=25), oropharynx (n=3), hypopharynx (n=13) and larynx (n=1). Objective response rate (ORR) with IC-PCE was 55.6%; 24 patients (88.9%) subsequently received CRT. ORR after completion of CRT was significantly better in the IC-PCE group (95.8% in the IC-PCE group vs. 66.7% in the CRT group, p=0.024). Progression-free survival (PFS) of the total population on median follow-up of 2.4 years (range: 0.8-7.3) tended to be better in the IC-PCE group (2-year PFS: 55.6% in the IC-PCE group vs. 33.3% in the CRT group, log-rank p=0.176), especially in oral cancer (2-year PFS: 37.5% in the IC-PCE group vs. 0% in the CRT group, log-rank p=0.015).ConclusionTherapeutic strategies including IC-PCE in patients with unresectable locoregional recurrent SCCHN after curative surgery may contribute to improved prognosis, especially in oral cancer.
Conventional management has a greater negative impact on Phaseolus vulgaris L. rhizobia diversity and abundance than water scarcity
Authors: del-Canto Arantza , Sanz-Saez Alvaro , Heath Katy D. , Grillo Michael A. , Heras Jónathan , Lacuesta Maite 
Published: July 2024

IntroductionDrought is one of the biggest problems for crop production and also affects the survival and persistence of soil rhizobia, which limits the establishment of efficient symbiosis and endangers the productivity of legumes, the main source of plant protein worldwide.AimSince the biodiversity can be altered by several factors including abiotic stresses or cultural practices, the objective of this research was to evaluate the effect of water availability, plant genotype and agricultural management on the presence, nodulation capacity and genotypic diversity of rhizobia.MethodA field experiment was conducted with twelve common bean genotypes under irrigation and rain-fed conditions, both in conventional and organic management. Estimation of the number of viable rhizobia present in soils was performed before the crop establishment, whereas the crop yield, nodule number and the strain diversity of bacteria present in nodules were determined at postharvest.ResultsRainfed conditions reduced the number of nodules and of isolated bacteria and their genetic diversity, although to a lesser extent than the agrochemical inputs related to conventional management. In addition, the effect of water scarcity on the conventional management soil was greater than observed under organic conditions.ConclusionsThe preservation of diversity will be a key factor to maintain crop production in the future, as problems caused by drought will be exacerbated by climate change and organic management can help to maintain the biodiversity of soil microbiota, a fundamental aspect for soil health and quality.
The mediating effects of moral disengagement and aggressive humor style: Dark Triad traits and schadenfreude
Authors: Sharafi Zadegan Milad , Pourhosein Reza , Azizi Zohreh 
Published: July 2024

BackgroundWhile schadenfreude is commonly experienced in interpersonal relationships, it is generally considered immoral. Although previous research has explored the factors relating to schadenfreude, including certain personality traits, moral disengagement, and humor styles, our understanding of these factors remains limited. The present study examined the mediating effects of moral disengagement and aggressive humor style in the relationship between the Dark Triad traits and schadenfreude.MethodsIn this study, a sample of 693 Iranian students (69.4% female), was recruited using a convenient sampling method, consisting of 330 undergraduate, 230 graduate, and 133 Ph.D. students. The trait schadenfreude scale, the short Dark Triad, the moral disengagement scale, and the aggressive humor style scale were measured through an online survey.ResultsThe study found that Machiavellianism, narcissism, and psychopathy were positively related to schadenfreude, aggressive humor style, and moral disengagement. Additionally, aggressive humor style and moral disengagement mediated the relationship between Machiavellianism, narcissism, and psychopathy with schadenfreude.ConclusionThis study provides insights into the complex relationships between Dark Triad traits, moral disengagement, aggressive humor style, and schadenfreude. The findings suggest that individuals with higher levels of Dark Triad traits may be more likely to experience pleasure from others’ misfortunes. Furthermore, the study highlights the importance of moral disengagement and aggressive humor style as potential mechanisms underlying the relationship between Dark Triad traits and schadenfreude. Further research should be done to explore the motivational factors influencing schadenfreude in specific settings, thereby elucidating these connections.
Alexithymia as a mediator between adverse childhood events and the development of psychopathology: a meta-analysis
Authors: Kick Lorenz , Schleicher Daniel , Ecker Angelika , Kandsperger Stephanie , Brunner Romuald , Jarvers Irina 
Published: July 2024

IntroductionVictims of child abuse have an elevated risk of developing mental health issues later in life. Several variables have been suggested as mediators of this correlation, but little is known about the possible influence of alexithymia. Alexithymia is a sub-clinical personality trait that manifests as difficulties recognizing and verbalizing emotions.MethodsIn this study, two separate meta-analyses were conducted using questionnaire data, and Pearson correlations for overall effects were estimated.ResultsThe correlation between child abuse and alexithymia showed to be significant (r= .26), as did the correlation between alexithymia and general psychopathology (r= .44). Further analyses revealed no indication for possible publication bias. When investigating differences between various subtypes of child maltreatment, each subtype significantly correlated with alexithymia. Emotional abuse, emotional neglect, and physical neglect had stronger correlations than physical and sexual abuse.DiscussionThese results suggest that alexithymia plays a mediating role, at least in part, in the relationship between experiences of child abuse and general psychopathology in adulthood. Therefore, alexithymia may be relevant to further research and deserves attention in the prevention of and therapy for mental health issues in victims of child abuse.
Recombinase polymerase amplification combined with lateral flow biosensor for rapid visual detection of Clostridium perfringens in chicken meat and milk
Authors: Tian Rui , Xie Feng , Liu Yuqing , Liu Guangjin , Li Qingxia , Wang Jinxiu , Zhang Hongjian , Dai Lei , Zhang Wei 
Published: July 2024

AimsClostridium perfringens is one of the major anaerobic pathogen causing food poisoning and animal enteritis. With the rise of antibiotic resistance and the restrictions of the use of antibiotic growth promoting agents (AGPs) in farming, Clostridium enteritis and food contamination have become more common. It is time-consuming and labor-intensive to confirm the detection by standard culture methods, and it is necessary to develop on-site rapid detection tools. In this study, a combination of recombinase polymerase amplification (RPA) and lateral flow biosensor (LFB) was used to visually detect C. perfringens in chicken meat and milk.Methods and resultsTwo sets of primers were designed for the plc gene of C. perfringens, and the amplification efficiency and specificity of the primers. Selection of primers produces an amplified fragment on which the probe is designed. The probe was combined with the lateral flow biosensor (LFB). The reaction time and temperature of RPA-LFB assay were optimized, and the sensitivity of the assay was assessed. Several common foodborne pathogens were selected to test the specificity of the established method. Chicken and milk samples were artificially inoculated with different concentrations (1 × 102 CFU/mL to 1 × 106 CFU/mL) of C. perfringens, and the detection efficiency of RPA-LFB method and PCR method was compared. RPA-LFB can be completed in 20 min and the results can be read visually by the LFB test strips. The RPA-LFB has acceptable specificity and the lowest detection limit of 100 pg./μL for nucleic acid samples. It was able to stably detect C. perfringens contamination in chicken and milk at the lowest concentration of 1 × 104 CFU/mL and 1 × 103 CFU/mL, respectively.ConclusionIn conclusion, RPA-LFB is specific and sensitive. It is a rapid, simple and easy-to-visualize method for the detection of C. perfringens in food and is suitable for use in field testing work.
Chemical Composition and In Vitro Bioactivities of Extracts from Cones of P. halepensis, P. brutia, and P. pinea: Insights into Pharmaceutical and Cosmetic Potential
Authors: Chammam Amel , Fillaudeau Luc , Romdhane Mehrez , Bouajila Jalloul 
Published: July 2024

Various parts of the Pinaceae species, a traditional plant, have potential health benefits and exhibit antibacterial, anti-cancer, and antioxidant activities. This study aims to investigate the biochemical properties of both petal (P) and core (C) fractions from pinecones of P. halepensis (PA), P. brutia (PB), and P. pinea (PP). Pinecones were manually separated into P and C, which were then milled to investigate maceration with solvents of increasing polarity: cyclohexane (1SV), ethyl acetate (2SV), and methanol (3SV) at 20 °C. Spectrophotometry was utilized to quantify the total phenolic content (TPC) and to assess bioactivities. Gas chromatography with mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were employed to identify the chemical composition. 3SV extracts demonstrated the highest TPC and a significant anti–oxidant potential. PA-P-3SV exhibited the highest TPC (460.66 mg GAE/g DW) and PP-P-3SV displayed the best IC50 (10.54 µg/mL) against DPPH. 1SV and 2SV extracts showed interesting anticancer activity against Hela and HepG2 cells. No significant toxic effect of P and C extracts from pinecones was observed on HEK-293 cells. GC-MS analysis unveiled 46 volatile compounds, of which 32 were detected for the first time in these species. HPLC analysis identified 38 compounds, of which 27 were not previously detected in these species. This study highlights the significant potential of pinecones as a rich source of bioactive compounds.
Pengaruh Brain Gym Therapy terhadap Tingkat Depresi Pasien Diabetes Melitus Tipe 2
Authors: Wahyuliniya Anak Agung Istri , Harini I Gusti Ayu , Candra I Wayan , Lestari Agus Sri 
Published: July 2024

Diabetes melitus (DM) is a serious chronic disease. DM is a major cause of many diseases, such as blindness, heart disease, and kidney failure. The incidence of DM in Indonesia is 2.8%, and Bali Province is 1,33%. DM patients can experience depression due to the need for long-term treatment to avoid complications. The purpose of this study was to determine the effect of brain gym therapy on depression levels in patients with type 2 DM. Pre experimental research design with One-group pre-post test design. The research sample was DM patients who experienced depression. The sampling technique used was purposive sampling. This research instrument uses the beck depression inventory II questionnaire. The statistical test used is the Wilcoxon test. Measurement of depression levels in DM patients was measured twice, namely before and after being given brain gym. The level of depression in research subjects before being given therapy in the moderate depression category, as many as 10 people (55,6%). The level of depression in the research subjects after being given therapy in the mild depression category, as many as 15 people (83,3%). Based on the results of the Wilcoxon test, the p value= 0,008 was obtained. The p value 0,05 indicates that there is an effect of brain gym therapy on depression in DM patients. The conclusion of this study is that brain gym reduces the level of depression in patients with type 2 DM.
APLIKASI R PADA AKURASI ESTIMASI OBSERVASI HILANG DALAM PENELITIAN RANCANGAN ACAK KELOMPOK
Authors: Pratignyo Lisana Sumarah , Prabowo Soekardi Hadi 
Published: July 2024

Abstrak.Tujuan Penelitian ini mengkaji Aplikasi Software R pada Akurasi Estimasi Observasi yang Hilang dalam penelitian Rancangan Acak Lengkap (RAK), serta Analisis Signifikansi Hasil Pengujian F-ANAVA/ Proses Estimasi observasi Hilang menggunakan metode Yates berdasarkan Prinsip Least Means Square (LMS). sSedangkan Akurasi estimasi observasi hilang menggunakan kriteria Means Absolute Percentage Error (MAPE).rPenelitian memberikan Hasil Akurasi estimasi observasi hilang menunjukkan masuk dalam kriteria Sangat Baik atau Akurat. Observasi Hilang berpengaruh pada Proses perhitungan Analisis Variansi, kehilangan dua observasi mengakibatkan derajat kebebasan berkurang 2 dan mempengaruni jumlah Kuadrat Residu sehingga menjadi kecil.