bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sorafenib perpetuates cellular anticancer effector functions by modulating the crosstalk between macrophages and natural killer cells
Authors: Sprinzl Martin Franz , Reisinger Florian , Puschnik Andreas , Ringelhan Marc , Ackermann Kerstin , Hartmann Daniel , Schiemann Matthias , Weinmann Arndt , Galle Peter Robert , Schuchmann Marcus , Friess Helmut , Otto Gerd , Heikenwalder Mathias , Protzer Ulrike 
Published: February 2013

Abstract

Alternatively polarized macrophages (Mφ) shape the microenvironment of hepatocellular carcinoma (HCC) and temper anticancer immune responses. We investigated if sorafenib alters the HCC microenvironment by restoring classical macrophage polarization and triggering tumor-directed natural killer (NK) cell responses.

In vivo

experiments were conducted with sorafenib (25 mg/kg)-treated C57BL/6 wildtype as well as hepatitis B virus (HBV) and lymphotoxin transgenic mice with and without HCC. Monocyte-derived Mφ or tumor-associated macrophages (TAM) isolated from HCC tissue were treated with sorafenib (0.07-5.0 μg/mL) and cocultured with autologous NK cells. Mφ and NK cell activation was analyzed by flow cytometry and killing assays, respectively. Cytokine and growth factor release was measured by enzyme-linked immunosorbent assay. Short-term administration of sorafenib triggered activation of hepatic NK cells in wildtype and tumor-bearing mice.

In vitro

, sorafenib sensitized Mφ to lipopolysaccharide, reverted alternative Mφ polarization and enhanced IL12 secretion (

P

= 0.0133). NK cells activated by sorafenib-treated Mφ showed increased degranulation (15.3 ± 0.2% versus 32.0 ± 0.9%,

P

< 0.0001) and interferon-gamma (IFN-γ) secretion (2.1 ± 0.2% versus 8.0 ± 0.2%,

P

< 0.0001) upon target cell contact. Sorafenib-triggered NK cell activation was verified by coculture experiments using TAM. Sorafenib-treated Mφ increased cytolytic NK cell function against K562, Raji, and HepG2 target cells in a dose-dependent manner. Neutralization of interleukin (IL)12 or IL18 as well as inhibition of the nuclear factor kappa B (NF-κB) pathway reversed NK cell activation in Mφ/NK cocultures.

Conclusion

: Sorafenib triggers proinflammatory activity of TAM and subsequently induces antitumor NK cell responses in a cytokine- and NF-κB-dependent fashion. This observation is relevant for HCC therapy, as sorafenib is a compound in clinical use that reverts alternative polarization of TAM in HCC.
Interstrain differences in chronic hepatitis and tumor development in a murine model of inflammation-mediated hepatocarcinogenesis
Authors: Potikha Tamara , Stoyanov Evgeniy , Pappo Orit , Frolov Antonina , Mizrahi Lina , Olam Deborah , Shnitzer-Perlman Temima , Weiss Ido , Barashi Neta , Peled Amnon , Sass Gabriele , Tiegs Gisa , Poirier Francoise , Rabinovich Gabriel A. , Galun Eithan , Goldenberg Daniel 
Published: February 2013

Abstract

Chronic inflammation is strongly associated with an increased risk for hepatocellular carcinoma (HCC) development. The multidrug resistance 2 (Mdr2)–knockout (KO) mouse (adenosine triphosphate–binding cassette b4−/−), a model of inflammation-mediated HCC, develops chronic cholestatic hepatitis at an early age and HCC at an adult age. To delineate factors contributing to hepatocarcinogenesis, we compared the severity of early chronic hepatitis and late HCC development in two Mdr2-KO strains: Friend virus B-type/N (FVB) and C57 black 6 (B6). We demonstrated that hepatocarcinogenesis was significantly less efficient in the Mdr2-KO/B6 mice versus the Mdr2-KO/FVB mice; this difference was more prominent in males. Chronic hepatitis in the Mdr2-KO/B6 males was more severe at 1 month of age but was less severe at 3 months of age in comparison with age-matched Mdr2-KO/FVB males. A comparative genome-scale gene expression analysis of male livers of both strains at 3 months of age revealed both common and strain-specific aberrantly expressed genes, including genes associated with the regulation of inflammation, the response to oxidative stress, and lipid metabolism. One of these regulators, galectin-1 (Gal-1), possesses both anti-inflammatory and protumorigenic activities. To study its regulatory role in the liver, we transferred the Gal-1–KO mutation (lectin galactoside-binding soluble 1−/−) from the B6 strain to the FVB strain, and we demonstrated that endogenous Gal-1 protected the liver against concanavalin A–induced hepatitis with the B6 genetic background but not the FVB genetic background. Conclusion: Decreased chronic hepatitis in Mdr2-KO/B6 mice at the age of 3 months correlated with a significant retardation of liver tumor development in this strain versus the Mdr2-KO/FVB strain. We found candidate factors that may determine strain-specific differences in the course of chronic hepatitis and HCC development in the Mdr2-KO model, including inefficient anti-inflammatory activity of the endogenous lectin Gal-1 in the FVB strain. (HEPATOLOGY 2013 )
Health care expenditures from living longer—how much do they matter
Authors: Gandjour Afschin 
Published: February 2013

ABSTRACTHealth interventions have two major downstream cost effects, savings from reducing morbidity and expenditures from living longer. Anecdotal evidence suggests that many economic evaluations, particularly those that are trial‐based, do not include health care costs from living longer. The purpose of this study was therefore to determine the bias from excluding life extension costs in economic evaluations. To this end, the impact of health changes on savings from preventing disease and costs of living longer was examined in the US Medicare population between 1998 and 2004. A state transition decision model with two health states (alive and dead) was built from an extended payer's perspective. It used Medicare expenditure data on survivors and decedents. Health changes were measured in terms of both morbidity and mortality reduction. The analysis shows that life extension costs cancel out savings from reducing morbidity. Users of economic evaluations may use this finding to estimate the bias when life extensions costs are not included in the analysis. Copyright © 2013 John Wiley & Sons, Ltd.
River classification as the basis for freshwater biological assessment in overseas Europe: Issues raised from Guadeloupe (French Lesser Antilles)
Authors: Touron‐Poncet Heliott , Bernadet Caroline , Compin Arthur , Bargier Nicolas , Céréghino Régis 
Published: February 2013

AbstractOver the past decade, Europe's Water Framework Directive (WFD) has prompted a large amount of ecological research aiming at establishing river typologies and ecological indicators in member States. Yet, the lack of robust bioindicators in Europe's overseas regions arguably reflects minimal knowledge of the distribution patterns of aquatic species in the Community's outermost areas. Specifically, there has been no published classification of rivers for any European overseas region. Fifty‐one sites were sampled for benthic invertebrates and environmental variables (land‐cover, physical habitat, and water chemistry) in Guadeloupe, French Lesser Antilles. Redundancy analysis and k‐means clustering were used to bring out spatial patterns in species composition in relation to environmental conditions. Our results highlighted the importance of land cover and geomorphology in delineating three ecological sub‐regions (clusters) for freshwater invertebrates. Deviation from predictable community structure only occurred when river sites were subjected to harsh water chemistry alterations (urban runoff, wastewaters). Changes in species richness did not detect environmental stress efficiently within a given sub‐region, probably because most sites are naturally species‐poor due to the insular context and/or because disturbance is often weak. However, differences existed between clusters in terms of species identity and numerical dominance. Our a posteriori typology of sites was compared to local a priori expert opinion of river health, in an attempt to better characterize the network of survey sites, and to target sites for reference conditions.
Parameterization of a reactive force field using a Monte Carlo algorithm
Authors: Iype E. , Hütter M. , Jansen A. P. J. , Nedea S. V. , Rindt C. C. M. 
Published: February 2013

AbstractParameterization of a molecular dynamics force field is essential in realistically modeling the physicochemical processes involved in a molecular system. This step is often challenging when the equations involved in describing the force field are complicated as well as when the parameters are mostly empirical. ReaxFF is one such reactive force field which uses hundreds of parameters to describe the interactions between atoms. The optimization of the parameters in ReaxFF is done such that the properties predicted by ReaxFF matches with a set of quantum chemical or experimental data. Usually, the optimization of the parameters is done by an inefficient single‐parameter parabolic‐search algorithm. In this study, we use a robust metropolis Monte‐Carlo algorithm with simulated annealing to search for the optimum parameters for the ReaxFF force field in a high‐dimensional parameter space. The optimization is done against a set of quantum chemical data for MgSO4hydrates. The optimized force field reproduced the chemical structures, the equations of state, and the water binding curves of MgSO4hydrates. The transferability test of the ReaxFF force field shows the extend of transferability for a particular molecular system. This study points out that the ReaxFF force field is not indefinitely transferable. © 2013 Wiley Periodicals, Inc.
Evaluation of the FilmArray® Respiratory Panel for Clinical Use in a Large Children's Hospital
Authors: Couturier Marc Roger , Barney Trenda , Alger Garrison , Hymas Weston C. , Stevenson Jeffery B. , Hillyard David , Daly Judy A. 
Published: February 2013

BackgroundRespiratory pathogens are a leading cause of hospital admission and traditional detection methods are time consuming and insensitive. Multiplex molecular detection methods have recently been investigated in hope of replacing these traditional techniques with rapid panel‐based testing.ObjectivesThis study evaluated the FilmArray® Respiratory Panel ([FARP], Idaho Technology Inc., Salt Lake City, UT) as a replacement for direct fluorescent antibody (DFA) testing in a pediatric hospital.MethodsEleven of the 21 FARP analytes (Adenovirus, Bordetella pertussis, human Metapneumovirus, Influenza A, Influenza A H1N1 2009, Influenza B, Parainfluenza [1, 2, & 3], Respiratory Syncytial Virus, and rhinovirus) were evaluated using nasopharyngeal specimens. Positive samples were pooled in groups of 5. Samples identified by reference methods as positive for respiratory pathogens were used for the majority of positive samples. DFA was the reference method for ten analytes; LuminexTM xTAG Respiratory Virus Panel (RVP) was the reference method for rhinovirus. Discrepant results were resolved by positive culture and fluorescent antibody stain and/or laboratory‐developed real‐time polymerase chain reaction (PCR) assays (LDT).ResultsThe agreement for most analytes was in concordance with the established reference methods with the exception of Adenovirus. Additionally, the FARP detected several pathogens not previously detected by DFA, and most were confirmed by LDT. Several DFA‐positive analytes were confirmed as true‐negatives by the FARP and LDT.ConclusionFARP overall performed better than DFA with the exception of Adenovirus, making the FARP an attractive alternative to laboratories looking to replace DFA with a rapid, user‐friendly, multiplex molecular assay. J. Clin. Lab. Anal. 27:148–154, 2013. © 2013 Wiley Periodicals, Inc.
Palladium recovery as nanoparticles by an anaerobic bacterial community
Authors: Martins Mónica , Assunção Ana , Martins Hélio , Matos António Pedro , Costa Maria Clara 
Published: February 2013

AbstractBACKGROUNDBio‐recovery of metals from dilute industrial waste using bacteria is a promising alternative to primary raw material resources. Therefore, the search for and identification of palladium resistant bacteria with the ability to remove that metal from solutions is very important. The main goal of this study was to find a palladium(II) resistant bacterial community able to bio‐recover this metal from solution.RESULTSA palladium(II) resistant bacterial community able to precipitate that metal from the growth medium was obtained from a sludge sample from a municipal waste water treatment plant. This community was able to remove 60% of palladium(II) from an aqueous solution containing 18 mg L‐1 of Pd(II) and 43% in the presence of 26 mg L‐1 Pd(II) plus sulphate. TEM‐EDS analysis showed the presence of nanosized palladium deposits in the surface of cells. Phylogenetic analysis of the 16S rRNA gene showed that this community was mainly composed of bacteria closely related to several Clostridium species. However, bacteria affiliated to genera Bacteroides and Citrobacter were also present in the community.CONCLUSIONThe current study for the first time reports the bio‐recovery of Pd(II) as nanoparticles by a bacterial consortium, and is a relevant demonstration of the biotechnological potentialities of this community. © 2013 Society of Chemical Industry
Analyzing the Effectiveness of Multisource Feedback as a Leadership Development Tool for College Students
Authors: Rosch David M. , Anderson James C. , Jordan Shannon N. 
Published: February 2013

AbstractUndergraduate students (n = 144) completed the Socially Responsible Leadership Scale (SRLS), which measures leadership capacity within the framework of the Social Change Model (SCM) of Leadership Development. Observers (n = 374) who knew the students from a variety of contexts completed observer‐report versions of the SRLS. The research was designed to measure differences in rating of capacity between student leaders and those who know and observe their behaviors. Mean observer scores were higher than self‐reported scores for most capacities, with moderate to large effect sizes. Significant differences between self and observer interscale correlations and a high degree of subscale intercorrelations within observer‐reports led to conducting an exploratory factor analysis of observer responses. A universal one‐factor structure emerged that was different from the SCM model, suggesting observers make little distinction among separate leadership capacities in those they observe. Implications for leadership development and education programs are discussed including the benefit of rater training prior to the use of multisource feedback instruments, how the context in which students interact with observers affects feedback, and the need for further examination of other methods of determining effectiveness in conjunction with multisource feedback instruments.
The pattern of cartilage damage in antero‐medial osteoarthritis of the knee and its relationship to the anterior cruciate ligament
Authors: Rout Raj , McDonnell Stephen , Hulley Philippa , Jayadev Chethan , Khan Tanvir , Carr Andrew , Murray David , Gill Harinderjit , Price Andrew 
Published: February 2013

AbstractWithin antero‐medial gonarthrosis (AMG) of the knee, there is a spectrum of damage seen in the functionally intact anterior cruciate ligament (ACL). Our aim was to correlate the degree of ACL damage to the geographical extent and degree of cartilage loss on the tibial plateau. Ninety tibial plateaus resected during unicompartmental arthroplasty were photographed and digitally mapped. The ACL damage was graded (0: normal, 1: synovium loss, 2: longitudinal splits), and dimensions of full thickness cartilage loss and damage recorded. The percentage of full thickness loss in patients with a normal ACL was compared to those with a damaged, but functionally intact ligament. All specimens showed similar elliptical loss of cartilage in the antero‐medial part of the tibial plateau. A total of 45(50%) patients had a macroscopically normal ACL, 21(23%) had synovial loss, and 24(27%) had longitudinal splits. An increase in the area of cartilage damage was seen with progressive ACL damage (p < 0.001). The area of macroscopically normal cartilage found posteriorly did not change. This study demonstrates that phenotypic distribution of cartilage damage in AMG is highly reproducible with a pattern of increasing cartilage erosion associated with increasing ACL damage. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 908–913, 2013
Effects of boron excess in nutrient solution on growth, mineral nutrition, and physiological parameters of Jatropha curcas seedlings
Authors: Simón Inma , Díaz‐López Leyanes , Gimeno Vicente , Nieves Manuel , Pereira Walter E. , Martínez Vicente , Lidon Vicente , García‐Sánchez Francisco 
Published: February 2013

AbstractJatropha curcas L. has recently attracted the attention of the international research community due to its potential as a biodiesel crop. In addition, its high resistance to drought and salinity is well known. Under arid and semiarid conditions, boron (B) concentrations in irrigation water can be higher than desired when water from industry, urban areas, or desalination is used. However, the growth and physiological responses of J. curcas plants to B excess in the irrigation water are unknown. Therefore, a greenhouse experiment was conducted to study the effects of B excess in the nutrient solution (0.25, 2, 4.5, and 7 mg L–1 B, applied as H3BO3) on plant growth, mineral concentration in the different plant tissues, photosynthesis, water relations, chlorophyll fluorescence, chlorophyll concentration (as SPAD values), and composition of carbohydrates. Plant growth decreased with increasing B concentration in the nutrient solution; growth reduction was higher for roots than for leaves or stems. The B concentration increased in all plant tissues, in the following order: leaf > root > stem. These data indicate that the roots of J. curcas are more sensitive to B toxicity than the leaves and that B has restricted mobility inside these plants, accumulating mainly in the basal and middle leaves via the transpiration stream. Increasing B concentration in leaves decreased the ACO2 and the stomatal conductance, but the leaf water parameters were not affected. The data for chlorophyll concentration and chlorophyll fluorescence indicated that nonstomatal factors were involved in the ACO2 decline, whereas decreases in the parameters of PSII photochemistry due to B toxicity suggest that there was structural damage in chloroplasts. There was also a general tendency for a decrease in nonstructural carbohydrates in all plant tissues, possibly due to the decline in ACO2. With excess B, the concentrations of K and Mg increased in leaves due to a decrease in the growth, while a typical antagonistic effect between B and P was evident from the P concentration decrease in leaves. In summary, J. curcas should be considered a B‐sensitive plant, as a leaf B concentration of 1.2 mg (g dw)–1 caused a growth decline of approximately 30%.
Patterns of effects of arbuscular mycorrhizal fungi on plants grown in contaminated soil
Authors: Neagoe Aurora , Iordache Virgil , Bergmann Hans , Kothe Erika 
Published: February 2013

AbstractArbuscular mycorrhizal fungi (AMF) are integral functioning parts of plant root systems and are widely recognized for enhancing plant growth on severely disturbed sites, including those contaminated with heavy metals. However, the generality of detailed patterns observed for their influence on various metals and oxidative‐stress parameters in multiple plant species is not clarified. The goal of this study was to investigate the patterns of metal‐stress alleviation by AMF in four plant species. For this purpose, clover, sunflower, mustard, and phacelia were inoculated with Glomus intraradices and compared to noninoculated plants grown under heavy metal–stressed conditions. The study focused on the effect of AMF inoculation on plant biomass, assimilating pigments, total protein, superoxide dismutase and peroxidase activity, lipid peroxidation and As, Cd, Co, Cu, Fe, Mn, P, Pb, U, and Zn contents. As a result of inoculation very different patterns of variation were obtained for concentrations of elements and for biochemical parameters in plants. The particular effect of AMF inoculation on plants was species‐ and metal‐specific, although there was a general enhancement of plant growth.
Separation and pre‐concentration of glucocorticoids in water samples by ionic liquid supported vortex‐assisted synergic microextraction and HPLC determination
Authors: Qin Hui , Li Bi , Liu Mou Sheng , Yang Ya Ling 
Published: February 2013

We have developed a synergic microextraction procedure based on ionic liquid for the pre‐concentration and determination of glucocorticoids in water samples. Using nonionic surfactant Triton X‐100 (TX‐100) as synergic reagent, 1‐butyl‐3‐methylimidazolium hexa‐fluorophosphate accomplished extraction rapidly without heating in water bath. One key property of ionic liquids that highlights their potential is their wide liquid temperature range. The improved extraction was named as ionic liquid supported vortex‐assisted synergic microextraction. Compared with the traditional liquid–liquid extraction and cloud point extraction, ionic liquid supported vortex‐assisted synergic microextraction was accomplished in 8 min with considerably high recovery. The proposed method greatly improved the sensitivity of HPLC for the determination of glucocorticoids. The results obtained indicated a good linearity with the correlation coefficient of 0.997 over the range of 0.6–300 ng/mL and high sensitivity with LODs of 4.11, 9.19, and 7.50 ng/mL for hydrocortisone butyrate, beclomethasone dipropionate, and nandrolone phenylpropionate, respectively. The RSD of the method was 1.57–1.81% (n = 6) with enrichment factor of 99.85, and good recovery (≥97.24%). The method was successfully applied to the determination of glucocorticoids in mineral water, water of Dianchi lake, and tap water samples.
Individual turnover in common pochards wintering in western France
Authors: Gourlay‐Larour Marie‐Lucile , Pradel Roger , Guillemain Matthieu , Santin‐Janin Hugues , L'hostis Monique , Caizergues Alain 
Published: February 2013

AbstractStudying movements and population turnover is a prerequisite of management and conservation policies. Understanding the relative importance of known wintering sites requires estimating total number of birds using a wintering site with robust statistical methods because counts alone do not account for turnover. To study movements of common pochards (Aythya ferina) during winter (Oct to Mar) in western France, we used a combination of band‐recoveries and capture–recapture data. We constructed a multi‐state model to estimate monthly local survival and probability of movements between the banding site and other wintering sites, accounting for individual sex and age. We observed significant movements between sites and high population turnover even during winter. We did not detect any effect of age or sex on movement probabilities, except at the beginning of the winter when first‐year individuals had a lower emigration probability. This result suggests that these inexperienced birds did not explore the wintering area like adults, at least early in the season. Combining our estimates (survival and emigration) and winter counts data, we computed the number of individuals using our study area throughout winter. This total was twice the maximum number of individuals counted in winter. Unexpectedly, low estimates for apparent survival suggest that permanent emigration occurred; although, recaptures and resightings revealed some birds traveled long distances from the capture site. We hypothesize that this permanent emigration was due to birds moving to areas where observation and hunting pressures were reduced compared to those in the French territory. If this hypothesis is true, high turnover rate would prevail not only at the scale of the wetland but also at the scale of the country. © The Wildlife Society, 2013