bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of arbuscular mycorrhiza fungal propagules on the growth of subterranean clover (Trifolium subterraneum L.) under Mn toxicity in ex situ experiments
Authors: Alho L. , Carvalho M. , Brito I. , Goss M. J. 
Published: April 2015

AbstractThe role of intact extraradical mycelium (ERM) as the most effective fungal propagule in the formation of the tripartite symbiosis between indigenous arbuscular mycorrhizal fungi (AMF), rhizobia and subterranean clover was investigated under conditions of Mn toxicity. ERM was previously developed in 8 L pots under greenhouse conditions by growing plants, which exhibited various levels of mycotrophicity and were tolerant to the levels of Mn in the soil used in the experiment (Silene gallica L, Lolium rigidum L, Ornithopus compressus L. and Rumex bucephalophorus L). Contrasting conditions of the integrity of the ERM at the planting of subterranean clover were created by soil disturbance (ERM fragmented – soil disturbed; ERM intact – soil undisturbed). Where an intact ERM was present at the time of planting, growth of subterranean clover was 2.5 times greater after 21 days and 3.9 times after 42 days relative to other forms of AMF propagule. This enhanced growth was associated with a reduction in the Mn concentration of roots due to a greater AMF colonization at 21 days after planting. The protection granted by an enhanced AMF root colonization allowed a greater root nodule development, leading to more N acquisition and plant growth. The ERM can be developed in the soil by mycotrophic plants tolerant to the stressing agent and kept intact at the seeding of the crop to be protected by adopting appropriate tillage techniques.
A future with less HLA: potential clinical applications of HLA‐universal cells
Authors: Figueiredo C. , Blasczyk R. 
Published: April 2015

AbstractThe high variability of the human leukocyte antigen (HLA) remains a major obstacle to the application of allogeneic products in cell‐based therapies. We have developed a strategy to decrease the immunogenicity of cell and tissues to improve their survival after allogeneic transplantation in the absence of immunosupression. Using RNA interference technology, the expression of HLA class I and II was stably downregulated. HLA‐silenced cells demonstrated to prevent a de novo and escape a pre‐formed alloimmune response in vitro and in vivo. Also, they demonstrated to be capable of engraft and survive after allogeneic transplantation independently of the donor's and recipient's genetic background. The generation of HLA‐universal cells has may open new horizons in the field of regenerative medicine. Some of the potential clinical applications of HLA universal cells will be discussed in this review.
RH diversity in Mali: characterization of a new haplotypeRHD*DIVa/RHCE*ceTI(D2)
Authors: Ba Alhassane , Beley Sophie , Chiaroni Jacques , Bailly Pascal , Silvy Monique 
Published: April 2015

BACKGROUNDKnowledge of RH variants in African populations is critical to improving transfusion safety in countries with populations of African ancestry and to providing valuable information and direction for future development of transfusion in Africa. The purpose of this report is to describe RH diversity in individuals from Mali.STUDY DESIGN AND METHODSBlood samples collected from 147 individuals self‐identified as Dogon and Fulani were analyzed for Rh antigens and alleles.RESULTSThe most commonRHDallele variant wasRHD*DAU0. Five predicted partial‐D phenotypes were attributed toRHD*DAU3orRHD*DIVa. NeitherRHD*DARnorRHD*DIIIawas found. Investigation ofRHCErevealed three predicted partial‐e antigens encoded byRHCE*ce(254G)in trans toRHCE*cE. Regarding C antigen, 28 Fulani typed as C+ and 16 of 28 harbored at least oneRHCE*Ce‐D(4)‐ce, two being homozygous and predicted to show a rare RH:32,−46 phenotype. A newRHCE*ceTIwith replacement of Exon 2 byRHD(RHCE*ceTI(D2))was identified in Dogon and was identified by inheritance study to be in cis toRHD*DIVa. These samples typed C– with anti‐C polyclonal antibody and monoclonal antibodies (MoAbs) MS24, P3X2551368+MS24, and MS273, but positive with anti‐RhCe MoAb‐BS58. The same pattern was observed in sample withRHD*DIVa/RHCE*ceTI.CONCLUSIONOur survey indicated an uneven distribution ofRHvariant alleles between Dogon and Fulani, suggesting that study in well‐documented cohorts is warranted. A high incidence of predicted partial‐C phenotype encoded byRHCE*Ce‐D(4)‐cewas found in Fulani. Further study will also be needed to clarify the clinical significance of the newDIVa/ceTI(D2)haplotype encoding partial D and variant ce antigens.
Complete Genome Sequences of vB_LmoS_188 and vB_LmoS_293, Two Bacteriophages with Specificity for Listeria monocytogenes Strains of Serotypes 4b and 4e
Authors: Casey Aidan , Jordan Kieran , Coffey Aidan , McAuliffe Olivia 
Published: April 2015

ABSTRACT

Listeria monocytogenes
is responsible for the rare disease listeriosis, which is associated with the consumption of contaminated food products. We report here the complete genome sequences of vB_LmoS_188 and vB_LmoS_293, phages isolated from environmental sources and that have host specificity for
L. monocytogenes
strains of the 4b and 4e serotypes.
Genome Sequence of Bluetongue Virus Type 2 from India: Evidence for Reassortment between Outer Capsid Protein Genes
Authors: Maan Sushila , Maan Narender S. , Belaganahalli Manjunatha N. , Kumar Aman , Batra Kanisht , Rao Pavuluri Panduranga , Hemadri Divakar , Reddy Yella Narasimha , Putty Kalyani , Krishnajyothi Yadlapati , Reddy G. Hanmanth , Singh Karam Pal , Hegde Nagendra R. , Nomikou Kyriaki , Sreenivasulu Daggupati , Mertens Peter P. C. 
Published: April 2015

ABSTRACT

Southern Indian isolate IND1994/01 of bluetongue virus serotype 2 (BTV-2), from the Orbivirus Reference Collection at the Pirbright Institute (
http://www.reoviridae.org/dsRNA_virus_proteins/ReoID/btv-2.htm#IND1994/01
), was sequenced. Its genome segment 6 (Seg-6) [encoding VP5(OCP2)] is identical to that of the Indian BTV-1 isolate (IND2003/05), while Seg-5 and Seg-9 are closely related to isolates from South Africa and the United States, respectively.
Identification of a Genotype VIId Newcastle Disease Virus Isolated from Sansui Sheldrake Ducks in Guizhou Province, China
Authors: Duan Zhiqiang , Ji Xinqin , Xu Houqiang , Zhao Jiafu , Ruan Yong , Chen Jiaqi 
Published: April 2015

ABSTRACT
In this study, we report the complete genome sequence of a novel Newcastle disease virus (NDV) strain, Sheldrake duck/China/Guizhou/SS1/2014, isolated from Sansui Sheldrake duck flocks in Guizhou Province, southwestern China. The genome of this isolate is 15,192 nucleotides in length, which belongs to NDV genotype VIId in class II. This discovery will help us further study the epidemiology characteristics and molecular pathogenesis of genotype VIId NDV in Sansui Sheldrake ducks.
De Novo
Whole-Genome Sequence of Micromonospora carbonacea JXNU-1 with Broad-Spectrum Antimicrobial Activity, Isolated from Soil Samples
Authors: Jiang Yun , Huang Yun-hong , Long Zhong-er 
Published: April 2015

ABSTRACT

Micromonospora carbonacea
JXNU-1 is an actinomycete with broad-spectrum antimicrobial activity, isolated from soil samples from the farmland in the area of Yaohu Lake in Nanchang, China. Here, we report the whole-genome sequence of M. carbonacea JXNU-1.
Draft Genome Sequence of NDM-5-Producing Escherichia coli Sequence Type 648 and Genetic Context of
bla
NDM-5
in Australia
Authors: Wailan Alexander M. , Paterson David L. , Caffery Michael , Sowden David , Sidjabat Hanna E. 
Published: April 2015

ABSTRACT

We report here the draft genome sequence of uropathogenic
Escherichia coli
sequence type 648 (ST648) possessing
bla
NDM-5
from a 55-year-old female in Australia with a history of travel to India. The plasmid-mediated
bla
NDM-5
was in a genetic context nearly identical to that of the GenBank entry of an IncX3
bla
NDM-5
plasmid previously reported from India (
Klebsiella pneumoniae
MGR-K194).
Draft Genome Sequence of Rhodotorula mucilaginosa, an Emergent Opportunistic Pathogen
Authors: Deligios Massimo , Fraumene Cristina , Abbondio Marcello , Mannazzu Ilaria , Tanca Alessandro , Addis Maria Filippa , Uzzau Sergio 
Published: April 2015

ABSTRACT

Rhodotorula mucilaginosa
, a yeast with valuable biotechnological features, has also been recorded as an emergent opportunistic pathogen that might cause disease in both immunocompetent and immunocompromised individuals. Here, we report the draft genome sequence of
R. mucilaginosa
strain C2.5t1, which was isolated from cacao seeds in Cameroon.
Draft Genome Sequence of Exophiala mesophila, a Black Yeast with High Bioremediation Potential
Authors: Tafer Hakim , Lopandic Ksenija , Blasi Barbara , Poyntner Caroline , Sterflinger Katja 
Published: April 2015

ABSTRACT

The fungal genus
Exophiala
comprises both pathogen species, which cause severe infections in humans, and environmental species, which are able to degrade alkylbenzene compounds. The draft genome sequence of Exophiala mesophila presented here is the first genome assembly of an alkylbenzene-degrading organism belonging to the genus
Exophiala
.
Draft Genome Sequences for Canadian Isolates of Pectobacterium carotovorum subsp. brasiliense with Weak Virulence on Potato
Authors: Li Xiang (Sean) , Yuan Kat (Xiaoli) , Cullis Jeff , Lévesque C. André , Chen Wen , Lewis Christopher T. , De Boer Solke H. 
Published: April 2015

ABSTRACT
Pectobacterium carotovurum subsp. brasiliense causes soft rot and blackleg diseases on potato. Here, we report the draft genome sequences of three weakly virulent P. carotovurum subsp. brasiliense strains isolated in Canada. Analysis of these genome sequences will help to pinpoint differences in virulence among P. carotovurum subsp. brasiliense strains from tropical/subtropical and temperate regions, such as Canada and United States. A small number of key factors for adaptation to this bacterium's specific environmental niche were also evaluated.
Genome Sequences of Two Bovine Mastitis-Causing Escherichia coli Strains
Authors: Kempf Florent , Loux Valentin , Germon Pierre 
Published: April 2015

ABSTRACT

Escherichia coli
is one of the main pathogenic agents causing inflammatory infections in the bovine udder. Here, we report the draft genome sequences of two strains isolated from different cases of clinical mastitis.
Draft Genome Sequence of Klebsiella pneumoniae Carbapenemase-Producing Acinetobacter baumannii Strain M3AC9-7, Isolated from Puerto Rico
Authors: Martínez Teresa , Ropelewski Alexander J. , González-Mendez Ricardo , Vázquez Guillermo J. , Robledo Iraida E. 
Published: April 2015

ABSTRACT

We report the draft genome of a multidrug resistant,
Klebsiella pneumoniae
carbapenemase (KPC)-producing
Acinetobacter baumannii
strain M3AC9-7 that belongs to the novel sequence type, ST250. The draft genome consists of a total length of 4.09 Mbp and a G+C content of 38.95%.
Complete Genome Sequence of Gardnerella vaginalis Strain JCM 11026
T
, Isolated from Vaginal Tracts of Women
Authors: Oshima Kenshiro , Hisamatsu Shin , Toh Hidehiro , Nakano Akiyo , Kiuchi Misa , Kuroyanagi Hiromi , Morita Hidetoshi , Hattori Masahira 
Published: April 2015

ABSTRACT

Gardnerella vaginalis
strain JCM 11026
T
was isolated from vaginal tracts of women. Here, we report the complete genome sequence of this organism.
Republished curriculum based clinical review: IBD: reproductive health, pregnancy and lactation
Authors: Hendy Philip , Chadwick Georgina , Hart Ailsa 
Published: April 2015

ABSTRACT
The peak incidence of inflammatory bowel diseases (IBD) occurs during the child-bearing years, and so questions about fertility, pregnancy and breast feeding are often asked by patients. It is known that patients with IBD receive twice as much information about pregnancy-related issues from gastroenterologists as from any other source (including the internet). Therefore, the role of the gastroenterologist in correctly educating patients to avoid misconceptions is paramount, and this should be done proactively prior to planning pregnancy so that the patient's health and medications can be optimised. A clear understanding of medication safety during pregnancy and lactation will improve clinical management.
TGFβ Is a Master Regulator of Radiation Therapy-Induced Antitumor Immunity
Authors: Vanpouille-Box Claire , Diamond Julie M. , Pilones Karsten A. , Zavadil Jiri , Babb James S. , Formenti Silvia C. , Barcellos-Hoff Mary Helen , Demaria Sandra 
Published: April 2015

AbstractT cells directed to endogenous tumor antigens are powerful mediators of tumor regression. Recent immunotherapy advances have identified effective interventions to unleash tumor-specific T-cell activity in patients who naturally develop them. Eliciting T-cell responses to a patient's individual tumor remains a major challenge. Radiation therapy can induce immune responses to model antigens expressed by tumors, but it remains unclear whether it can effectively prime T cells specific for endogenous antigens expressed by poorly immunogenic tumors. We hypothesized that TGFβ activity is a major obstacle hindering the ability of radiation to generate an in situ tumor vaccine. Here, we show that antibody-mediated TGFβ neutralization during radiation therapy effectively generates CD8+ T-cell responses to multiple endogenous tumor antigens in poorly immunogenic mouse carcinomas. Generated T cells were effective at causing regression of irradiated tumors and nonirradiated lung metastases or synchronous tumors (abscopal effect). Gene signatures associated with IFNγ and immune-mediated rejection were detected in tumors treated with radiation therapy and TGFβ blockade in combination but not as single agents. Upregulation of programmed death (PD) ligand-1 and -2 in neoplastic and myeloid cells and PD-1 on intratumoral T cells limited tumor rejection, resulting in rapid recurrence. Addition of anti–PD-1 antibodies extended survival achieved with radiation and TGFβ blockade. Thus, TGFβ is a fundamental regulator of radiation therapy's ability to generate an in situ tumor vaccine. The combination of local radiation therapy with TGFβ neutralization offers a novel individualized strategy for vaccinating patients against their tumors. Cancer Res; 75(11); 2232–42. ©2015 AACR.
Exercícios gramaticais no ensino de paisagismo experiências didáticas em diferentes contextos: UFU/Uberlância e UNIFOR/Fortaleza
Authors: Cocozza Glauco de Paula , Rocha Fernanda Cláudia Lacerda 
Published: April 2015

<p>Este artigo apresenta alguns exercícios gramaticais aplicados na disciplina de Paisagismo da Universidade Federal de Uberlândia e da Universidade de Fortaleza, apresentando a metodologia aplicada, os temas sugeridos para os exercícios, a sua relação com a ementa da disciplina, os reflexos no aprendizado, e como eles possibilitam uma interpretação dos conceitos e práticas que o paisagismo propicia. Essa temática não é recente, alguns professores adotam metodologias semelhantes, variando o tema, a articulação com conteúdos ministrados e a forma de abordagens. O objetivo deste trabalho é apresentar como os exercícios são pensados na disciplina, como são executados e de que modo se relacionam ao trabalho final entregue como síntese da disciplina de Paisagismo.</p>
Dimethylarginine Dimethylaminohydrolase 2 Regulates Nitric Oxide Synthesis and Hemodynamics and Determines Outcome in Polymicrobial Sepsis
Authors: Lambden Simon , Kelly Peter , Ahmetaj-Shala Blerina , Wang Zhen , Lee Benjamin , Nandi Manasi , Torondel Belen , Delahaye Matthew , Dowsett Laura , Piper Sophie , Tomlinson James , Caplin Ben , Colman Lucy , Boruc Olga , Slaviero Anna , Zhao Lan , Oliver Eduardo , Khadayate Sanjay , Singer Mervyn , Arrigoni Francesca , Leiper James 
Published: April 2015

Objective—

Nitric oxide is a key to numerous physiological and pathophysiological processes. Nitric oxide production is regulated endogenously by 2 methylarginines, asymmetric dimethylarginine (ADMA) and monomethyl-
l
-arginine. The enzyme that specifically metabolizes asymmetric dimethylarginine and monomethyl-
l
-arginine is dimethylarginine dimethylaminohydrolase (DDAH). The first isoform dimethylarginine dimethylaminohydrolase 1 has previously been shown to be an important regulator of methylarginines in both health and disease. This study explores for the first time the role of endogenous dimethylarginine dimethylaminohydrolase 2 in regulating cardiovascular physiology and also determines the functional impact of dimethylarginine dimethylaminohydrolase 2 deletion on outcome and immune function in sepsis.

Approach and Results—

Mice, globally deficient in Ddah2, were compared with their wild-type littermates to determine the physiological role of Ddah2 using in vivo and ex vivo assessments of vascular function. We show that global knockout of Ddah2 results in elevated blood pressure during periods of activity (mean [SEM], 118.5 [1.3] versus 112.7 [1.1] mm Hg;
P
=0.025) and changes in vascular responsiveness mediated by changes in methylarginine concentration, mean myocardial tissue asymmetric dimethylarginine (SEM) was 0.89 (0.06) versus 0.67 (0.05) μmol/L (
P
=0.02) and systemic nitric oxide concentrations. In a model of severe polymicrobial sepsis, Ddah2 knockout affects outcome (120-hour survival was 12% in Ddah2 knockouts versus 53% in wild-type animals;
P
<0.001). Monocyte-specific deletion of Ddah2 results in a similar pattern of increased severity to that seen in globally deficient animals.

Conclusions—
Ddah2 has a regulatory role both in normal physiology and in determining outcome of severe polymicrobial sepsis. Elucidation of this role identifies a mechanism for the observed relationship between Ddah2 polymorphisms, cardiovascular disease, and outcome in sepsis.
Enhanced Pulmonary Vasodilator Reserve and Abnormal Right Ventricular
Authors: Andersen Mads J. , Hwang Seok-Jae , Kane Garvan C. , Melenovsky Vojtech , Olson Thomas P. , Fetterly Kenneth , Borlaug Barry A. 
Published: April 2015

Background—
Pulmonary hypertension and right ventricular (RV) dysfunction are common in patients with advanced heart failure with preserved ejection fraction (HFpEF), yet their underlying mechanisms remain poorly understood. We sought to examine RV–pulmonary artery (PA) functional reserve responses and RV–PA coupling at rest and during β-adrenergic stimulation in subjects with earlier stage HFpEF.

Methods and Results—

In a prospective trial, subjects with HFpEF (n=39) and controls (n=18) underwent comprehensive invasive and noninvasive hemodynamic assessment using high fidelity micromanometer catheters, echocardiography, and expired gas analysis at rest and during dobutamine infusion. HFpEF subjects displayed similar RV structure but significantly impaired RV systolic (lower RV d
P
/d
t
max
/IP and s′) and diastolic function (higher RV τ) coupled with more severe pulmonary vascular disease, manifest by higher PA pressures, higher PA resistance, and lower PA compliance compared with controls. Dobutamine infusion caused greater pulmonary vasodilation in HFpEF compared with controls, with enhanced reductions in PA resistance, greater increase in PA compliance, and a more negative slope in the PA pressure–flow relationship when compared with controls (all
P
<0.001). RV–PA coupling analysis revealed that dobutamine improved RV ejection in HFpEF subjects through afterload reduction alone, rather than through enhanced contractility, indicating RV systolic reserve dysfunction.

Conclusions—
Pulmonary hypertension in early stage HFpEF is related to partially reversible pulmonary vasoconstriction coupled with RV systolic and diastolic dysfunction, even in the absence of RV structural remodeling. Pulmonary vascular tone is more favorably responsive to β-adrenergic stimulation in HFpEF than controls, suggesting a potential role for β-agonists in the treatment of patients with HFpEF and pulmonary hypertension.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT01418248.
Cerebrovascular Events After a Primary Percutaneous Coronary Intervention Strategy for Acute ST-Segment– Elevation Myocardial Infarction
Authors: Nikolsky Eugenia , Mehran Roxana , Dangas George D. , Xu Ke , Parvataneni Rupa , Witzenbichler Bernhard , Guagliumi Giulio , Kornowski Ran , Généreux Philippe , Brener Sorin J. , Stone Gregg W. 
Published: April 2015

Background—
Patients with ST-segment–elevation myocardial infarction are at increased risk of cerebrovascular events. We assessed the incidence, predictors, and implications of cerebrovascular events in patients with ST-segment–elevation myocardial infarction managed with a primary percutaneous coronary intervention strategy.

Methods and Results—

In the Harmonizing Outcomes With Revascularization and Stents in Acute Myocardial Infarction (HORIZONS-AMI) trial, 72 of 3602 patients (2.0%) experienced at least 1 cerebrovascular event (stroke: 63 patients; transient ischemic attack: 12 patients) during the 3-year follow-up (40.3% within 30 days, 20.8% between 30 days and 1 year, and 38.9% between 1 and 3 years). Stroke was ischemic in 58 (92.1%) patients and hemorrhagic in 5 (7.9%) patients. More than half of all strokes (52.3%) were disabling. By principal management strategy, cerebrovascular events developed in 2.0%, 14.9%, and 1.9% of patients triaged to primary percutaneous coronary intervention, coronary artery bypass grafting, and medical therapy, respectively (
P
<0.0001). Cerebrovascular events were independently predicted by older age, creatinine clearance <60 mL/min, treatment with coronary artery bypass grafting, anemia, and diabetes mellitus. Cerebrovascular events were associated with significantly increased rates of 3-year mortality (20.5% versus 6.5%;
P
<0.0001), as well as reinfarction (14.3% versus 3.8%;
P
=0.0007), ischemia-driven target vessel revascularization (22.8% versus 13.0%;
P
=0.006), and major bleeding (23.5% versus 8.4%;
P
<0.0001).

Conclusions—
In HORIZONS-AMI, cerebrovascular events within 3 years after ST-segment–elevation myocardial infarction in patients undergoing a primary percutaneous coronary intervention management strategy occurred in 2.0% of patients and were most frequent after coronary artery bypass grafting. Cerebrovascular events were often disabling and were strongly associated with high rates of death, reinfarction, recurrent ischemia, and major bleeding.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT00433966.