bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cats and Dogs: Two Neglected Species in this Era of Embryo Production in Vitro?
Authors: Van Soom A , Rijsselaere T , Filliers M 
Published: June 2014

ContentsDuring the last decades, in vitro fertilization (IVF) has become a routine technique in most domestic animals. However, in the dog the technique has lagged behind, with to date not a single pup born after IVF. In cats, healthy kittens have been born, but in fewer numbers than in cattle and horses. In pet animals, research in reproduction has mainly been focused on contraception, although recently, the introduction of new drugs especially marketed for cats and dogs will probably expand fertility research in carnivores towards the previously neglected area of assisted reproduction. In particular, the dog remains a real challenge for the reproductive biologist, due to the low meiotic capacity of canine follicular oocytes. In cats, oocyte maturation is less of a problem and embryo production rates comparable to those of cattle can be achieved. The domestic cat is a valuable model for endangered felids and it can even be used as a recipient for wild felid embryos. In this short review, we list some of the problems associated with the implementation of IVF in dogs and cats in relation to their reproductive characteristics, and we discuss the state‐of‐the‐art of IVF in several other domestic species such as cattle, horses and pigs.
Causes, Diagnosis and Therapy of Common Diseases in Neonatal Puppies in the First Days of Life: Cornerstones of Practical Approach
Authors: Münnich A , Küchenmeister U 
Published: June 2014

ContentsNeonatal diseases and losses are a common and often unavoidable problem within breeding kennels. Altogether, morbidity and mortality ranges, according to the literature, from 5 to 35%. Among non‐infectious causes besides hypoxia during birth, hypothermia, hypoglycaemia and dehydration are mostly responsible for puppy diseases and losses. Approximately 90% of all deaths in hypoxaemic pups occur during the first 2 days. Of 183 pups with hypoxia, 63 died, 92.7% of them within 48 h after birth. Among infectious causes, bacterial infection is the most common cause of neonatal mortality. Escherichia coli, streptococci, staphylococci, Pseudomonas sp., Klebsiella sp., Enterobacter sp. and some other micro‐organisms are regularly involved in neonatal infections. Post‐mortem findings especially document E. coli, Staphylococcus sp. and Streptococcus sp. as responsible bacteria. The dam and the environment are suspected as sources of neonatal infections as it was shown by genetic relatedness of responsible bacterial strains isolated in both puppies and their dams. From a total of 517 puppies with bacterial infections, the treatment results documented that parenteral administration of amoxicillin/clavulanic acid in 308 neonates showed the best result. Diagnosis of diseases is often made difficult by the absence of variability in clinical signs contrary to adult dogs. Findings during a physical examination in pups differ from those in adults. Furthermore, treatment recommendations have to meet the special conditions in neonates concerning drug metabolism and excretion.
Diagnostic and Prognostic Markers for Uterine Diseases in Dogs
Authors: Hagman R 
Published: June 2014

ContentsCommon uterine diseases in female dogs include cystic endometrial hyperplasia (CEH), mucometra, hydrometra and pyometra. It is important in clinical practice to recognize pyometra because it is potentially life‐threatening due to the systemic illness induced by bacterial infection of the uterus. In contrast, the uterine content is sterile in CEH and mucometra/hydrometra, and clinical signs are mostly mild or absent. Optimal treatment depends on the type of uterine disease and its severity, but diagnosis and prognosis determination may be challenging and the diseases difficult to separate clinically. Diagnostic findings or biomarkers that may aid in the differentiation of the diseases are valuable, especially when several bitches are admitted with a fluid‐filled uterus during night‐time, and it has to be decided which patient to operate on first. Additionally, some variables may indicate outcome as measured by mortality or morbidity. If the uterus is not enlarged or fluid‐filled, detection of uterine disease can be even more difficult. In this study, clinically useful variables with possible diagnostic or prognostic value for uterine diseases in dogs are discussed.
Interventional therapies for malignant pleural effusions: The present and the future
Authors: Thomas Rajesh , Francis Roslyn , Davies Helen E. , Lee Y.C. Gary 
Published: June 2014

AbstractThe approach to management of malignant pleural effusions (MPE) has changed over the past few decades. The key goals of MPE management are to relieve patient symptoms using the least invasive means and in the most cost‐effective manner. There is now a realization that patient‐reported outcome measures should be the primary goal of MPE treatment, and this now is the focus in most clinical trials. Efforts to minimize patient morbidity are complemented by development of less invasive treatments that have mostly replaced the more aggressive surgical approaches of the past. Therapeutic thoracentesis is simple, effective and generally safe, although its benefits may only be temporary. Pleurodesis is the conventional and for a long time the only definitive therapy available. However, the efficacy and safety of talc pleurodesis has been challenged. Indwelling pleural catheter (IPC) drainage is increasingly accepted worldwide and represents a new concept to improve symptoms without necessarily generating pleural symphysis. Recent studies support the effectiveness of IPC treatment and provide reassurance regarding its safety. An unprecedented number of clinical trials are now underway to improve various aspects of MPE care. However, choosing an optimal intervention for MPE in an individual patient remains a challenge due to our limited understanding of the underlying pathophysiology of breathlessness in MPE and a lack of predictors of survival and pleurodesis outcome. This review provides an overview of common pleural interventional procedures used for MPE management, controversies and limitations of current practice, and areas of research most needed to improve practice in future.
Fossil water striders in Cretaceous French amber (Heteroptera: Gerromorpha: Mesoveliidae and Veliidae)
Authors: SOLÓRZANO KRAEMER MÓNICA M. , PERRICHOT VINCENT , SORIANO CARMEN , DAMGAARD JAKOB 
Published: June 2014

AbstractFossil gerromorphan bugs from the Cretaceous (Albian–Cenomanian boundary) amber of Charentes, SW France, are reviewed. A larva described by Perrichot et al. (2005) as incertae familiae within the Gerromorpha is now placed in the Mesoveliidae. Three new genera and species are also described and illustrated: Emilianovelia audax Solórzano Kraemer & Perrichot, gen. et sp.n., and Malenavelia videris Solórzano Kraemer & Perrichot, gen. et sp.n., which are placed in the Mesoveliidae: Mesoveliinae; and Arcantivelia petraudi Solórzano Kraemer & Perrichot, gen. et sp.n., which is the first Mesozoic member of the Veliinae. The relationships between these fossils and their palaeoecology are discussed. The new discoveries confirm the antiquity of the semi‐aquatic gerromorphans, particularly the clade (Veliidae + Gerridae). The habitat is described as aquatic and likely marine‐influenced, yet adaptation to a fully marine habitat in these gerromorphans remains difficult to establish.This published work has been registered in ZooBank, http://zoobank.org/urn:lsid:zoobank.org:pub:F8C034B8-7BD0-4C03-B8FC-CD81D75C7001.
Leaf phenomics: a systematic reverse genetic screen for Arabidopsis leaf mutants
Authors: Wilson‐Sánchez David , Rubio‐Díaz Silvia , Muñoz‐Viana Rafael , Pérez‐Pérez José Manuel , Jover‐Gil Sara , Ponce María Rosa , Micol José Luis 
Published: June 2014

SummaryThe study and eventual manipulation of leaf development in plants requires a thorough understanding of the genetic basis of leaf organogenesis. Forward genetic screens have identified hundreds of Arabidopsis mutants with altered leaf development, but the genome has not yet been saturated. To identify genes required for leaf development we are screening the Arabidopsis Salk Unimutant collection. We have identified 608 lines that exhibit a leaf phenotype with full penetrance and almost constant expressivity and 98 additional lines with segregating mutant phenotypes. To allow indexing and integration with other mutants, the mutant phenotypes were described using a custom leaf phenotype ontology. We found that the indexed mutation is present in the annotated locus for 78% of the 553 mutants genotyped, and that in half of these the annotated T‐DNA is responsible for the phenotype. To quickly map non‐annotated T‐DNA insertions, we developed a reliable, cost‐effective and easy method based on whole‐genome sequencing. To enable comprehensive access to our data, we implemented a public web application named PhenoLeaf (http://genetics.umh.es/phenoleaf) that allows researchers to query the results of our screen, including text and visual phenotype information. We demonstrated how this new resource can facilitate gene function discovery by identifying and characterizing At1g77600, which we found to be required for proximal–distal cell cycle‐driven leaf growth, and At3g62870, which encodes a ribosomal protein needed for cell proliferation and chloroplast function. This collection provides a valuable tool for the study of leaf development, characterization of biomass feedstocks and examination of other traits in this fundamental photosynthetic organ.
Mechanisms of physiological adjustment of N2 fixation in Cicer arietinum L. (chickpea) during early stages of water deficit: single or multi‐factor controls
Authors: Nasr Esfahani Maryam , Sulieman Saad , Schulze Joachim , Yamaguchi‐Shinozaki Kazuko , Shinozaki Kazuo , Tran Lam‐Son Phan 
Published: June 2014

SummaryDrought negatively impacts symbiotic nitrogen fixation (SNF) in Cicer arietinum L. (chickpea), thereby limiting yield potential. Understanding how drought affects chickpea nodulation will enable the development of strategies to biotechnologically engineer chickpea varieties with enhanced SNF under drought conditions. By analyzing carbon and nitrogen metabolism, we studied the mechanisms of physiological adjustment of nitrogen fixation in chickpea plants nodulated with Mesorhizobium ciceri during both drought stress and subsequent recovery. The nitrogenase activity, levels of several key carbon (in nodules) and nitrogen (in both nodules and leaves) metabolites and antioxidant compounds, as well as the activity of related nodule enzymes were examined in M. ciceri‐inoculated chickpea plants under early drought stress and subsequent recovery. Results indicated that drought reduced nitrogenase activity, and that this was associated with a reduced expression of the nifK gene. Furthermore, drought stress promoted an accumulation of amino acids, mainly asparagine in nodules (but not in leaves), and caused a cell redox imbalance in nodules. An accumulation of organic acids, especially malate, in nodules, which coincided with the decline of nodulated root respiration, was also observed under drought stress. Taken together, our findings indicate that reduced nitrogenase activity occurring at early stages of drought stress involves, at least, the inhibition of respiration, nitrogen accumulation and an imbalance in cell redox status in nodules. The results of this study demonstrate the potential that the genetic engineering‐based improvement of SNF efficiency could be applied to reduce the impact of drought on the productivity of chickpea, and perhaps other legume crops.
Nucleotide polymorphism and copy number variant detection using exome capture and next‐generation sequencing in the polyploid grass Panicum virgatum
Authors: Evans Joseph , Kim Jeongwoon , Childs Kevin L. , Vaillancourt Brieanne , Crisovan Emily , Nandety Aruna , Gerhardt Daniel J. , Richmond Todd A. , Jeddeloh Jeffrey A. , Kaeppler Shawn M. , Casler Michael D. , Buell C. Robin 
Published: June 2014

SummarySwitchgrass (Panicum virgatum) is a polyploid, outcrossing grass species native to North America and has recently been recognized as a potential biofuel feedstock crop. Significant phenotypic variation including ploidy is present across the two primary ecotypes of switchgrass, referred to as upland and lowland switchgrass. The tetraploid switchgrass genome is approximately 1400 Mbp, split between two subgenomes, with significant repetitive sequence content limiting the efficiency of re‐sequencing approaches for determining genome diversity. To characterize genetic diversity in upland and lowland switchgrass as a first step in linking genotype to phenotype, we designed an exome capture probe set based on transcript assemblies that represent approximately 50 Mb of annotated switchgrass exome sequences. We then evaluated and optimized the probe set using solid phase comparative genome hybridization and liquid phase exome capture followed by next‐generation sequencing. Using the optimized probe set, we assessed variation in the exomes of eight switchgrass genotypes representing tetraploid lowland and octoploid upland cultivars to benchmark our exome capture probe set design. We identified ample variation in the switchgrass genome including 1 395 501 single nucleotide polymorphisms (SNPs), 8173 putative copy number variants and 3336 presence/absence variants. While the majority of the SNPs (84%) detected was bi‐allelic, a substantial number was tri‐allelic with limited occurrence of tetra‐allelic polymorphisms consistent with the heterozygous and polyploid nature of the switchgrass genome. Collectively, these data demonstrate the efficacy of exome capture for discovery of genome variation in a polyploid species with a large, repetitive and heterozygous genome.
Roles of obstetricians in quality management of cord blood collection and informing potential donors: a survey on obstetricians' understanding of cord blood in Korea
Authors: Roh Eun Youn , Shin Sue , Kim Byoung Jae , Kim Mina , Hong Jung Ja , Yoon Jong Hyun 
Published: June 2014

BackgroundThe obstetrician's role in vitalizing cord blood (CB) banking is important in both informing donors and beginning CB processing. The aim of the study was to investigate obstetricians' understanding of CB and their roles in informing donors and collection management.Study Design and MethodsQuestionnaires examining the management and awareness of CB were distributed to collection centers, and we analyzed 57 respondents who had collection experience.ResultsAll respondents were experienced, and 82.5% had CB collection experience of at least 4 years. If patients asked for information, one‐third provided information about CB themselves, and two‐thirds provided CB banks' (CBBs') contact information. Less than half of the respondents knew the legal regulations and had a collection manual. Regarding the awareness of transportation and storage of CB, 58% rated their knowledge as minimal, and respondents who knew the legal regulations rated their knowledge at a higher level. One‐fifth of the participants felt that CB collection distracted the labor process, and 16% were concerned about delayed bleeding control. The probability of autologous CB transplantation was overestimated, and the matching probability in public CBBs was underestimated. Approximately 82.7% of the respondents correctly rated the therapeutic use of CB, and 54% rated their knowledge level about CB usefulness as average.ConclusionObstetricians' understanding of CB was lower than expected, considering that they are associated with CB collection centers. Obstetricians who provide information and recognize regulations seem to collect CB in compliance with the regulations better. These results will be helpful in CB collection management and education planning for obstetricians.
Metric Diophantine approximation on homogeneous varieties
Authors: Ghosh Anish , Gorodnik Alexander , Nevo Amos 
Published: June 2014

AbstractWe develop the metric theory of Diophantine approximation on homogeneous varieties of semisimple algebraic groups and prove results analogous to the classical Khintchine and Jarník theorems. In full generality our results establish simultaneous Diophantine approximation with respect to several completions, and Diophantine approximation over general number fields using$\def \xmlpi #1{}\def \mathsfbi #1{\boldsymbol {\mathsf {#1}}}\let \le =\leqslant \let \leq =\leqslant \let \ge =\geqslant \let \geq =\geqslant \def \Pr {\mathit {Pr}}\def \Fr {\mathit {Fr}}\def \Rey {\mathit {Re}}S$-algebraic integers. In several important examples, the metric results we obtain are optimal. The proof uses quantitative equidistribution properties of suitable averaging operators, which are derived from spectral bounds in automorphic representations.
Genome Sequence of Abbey Lake Virus, a Novel Orthobunyavirus Isolated from China
Authors: Liu Ran , Zhang Guilin , Yang Yinhui , Dang Rongli , Zhao Tongyan 
Published: June 2014

ABSTRACT

The
Orthobunyavirus
genus of
Bunyaviridae
is a divergent group of medically important pathogens. Abbey Lake bunyavirus (Ab-BUNV) was newly isolated and identified in Xinjiang Province, northwestern China. The complete genome of Ab-BUNV was sequenced and is reported here, revealing that Ab-BUNV may represent a novel genotype in the genus
Orthobunyavirus
.
Genome Sequences of Nine Bordetella holmesii Strains Isolated in the United States
Authors: Harvill Eric T. , Goodfield Laura L. , Ivanov Yury , Smallridge William E. , Meyer Jessica A. , Cassiday Pamela K. , Tondella Maria L. , Brinkac Lauren , Sanka Ravi , Kim Maria , Losada Liliana 
Published: June 2014

ABSTRACT

An increasing number of pertussis-like cases are attributed to the emergent pathogen
Bordetella holmesii
. The genomes of 9 clinical isolates show that they are clonal, lack the virulence factors encoded by
B. pertussis
, and are more similar to nonpertussis bordetellae. New markers for
B. holmesii
can be developed using these sequences.
Full-Genome Analysis of Influenza A(H7N9) Virus from Shanghai, China, 2014
Authors: Zhang Wanju , He Yanchao , Xu Lei , Dai Fahui , Mei Zhoufang , Qian Ling , Xie Desheng , Shen Ying , Gu Yong , Zhang Zhiyong , Yuan Zhenghong , Jie Zhijun , Hu Yunwen 
Published: June 2014

ABSTRACT
We analyzed the complete genome sequence of the A/Shanghai/01/2014 (H7N9) strain, which will provide a better understanding of the evolution of influenza A(H7N9) virus.
Genome Sequence of
meso
-2,3-Butanediol-Producing Strain Serratia marcescens ATCC 14041
Authors: Li Lixiang , Wang Yu , Li Kun , Su Fei , Ma Cuiqing , Xu Ping 
Published: June 2014

ABSTRACT

Serratia marcescens
strain ATCC 14041 was found to be an efficient
meso
-2,3-butanediol (
meso
-2,3-BD) producer from glucose and sucrose. Here we present a 5.0-Mb assembly of its genome. We have annotated 4 coding sequences (CDSs) for
meso
-2,3-BD fermentation and 2 complete operons including 6 CDSs for sucrose utilization.
Complete Genome Sequence of a Velogenic Newcastle Disease Virus Isolated from an Apparently Healthy Village Chicken in South India
Authors: Uthrakumar Arumugam , Vijayarani Kumanan , Kumanan Kathaperumal , Bhuvaneswari Srinivasan , Kuchipudi Suresh Varma , Elankumaran Subbiah 
Published: June 2014

ABSTRACT
We report the complete genome sequence of a Newcastle disease virus (NDV) isolate, NDV-D1/1998, from an apparently healthy village chicken in South India. This class II, genotype II virus is 15,186 nucleotides in length with unique amino acid variations and was found to be a velogenic pathotype by standard pathogenicity tests.
Draft Genome Sequence of Enterococcus faecalis MB5259
Authors: Karlyshev Andrey V. , Robyn Joris , Rasschaert Geertrui , Heyndrickx Marc 
Published: June 2014

ABSTRACT

In this study, we present a draft genome sequence of
Enterococcus faecalis
MB5259, a promising probiotic strain. The identified differences and common features between this strain and reference strains will assist in better understanding the mechanism of antibacterial action and in developing novel probiotics.
Draft Genome Sequence of the Lignin-Degrading
Burkholderia
sp. Strain LIG30, Isolated from Wet Tropical Forest Soil
Authors: Woo Hannah L. , Utturkar Sagar , Klingeman Dawn , Simmons Blake A. , DeAngelis Kristen M. , Brown Steven D. , Hazen Terry C. 
Published: June 2014

ABSTRACT

Burkholderia
species are common soil
Betaproteobacteria
capable of degrading recalcitrant aromatic compounds and xenobiotics.
Burkholderia
sp. strain LIG30 was isolated from wet tropical forest soil and is capable of utilizing lignin as a sole carbon source. Here we report the draft genome sequence of
Burkholderia
sp. strain LIG30.
Cardiovascular screening in adolescents and young adults: a prospective study comparing the Pre-participation Physical Evaluation Monograph 4th Edition and ECG
Authors: Fudge Jessie , Harmon Kimberly G , Owens David S , Prutkin Jordan M , Salerno Jack C , Asif Irfan M , Haruta Alison , Pelto Hank , Rao Ashwin L , Toresdahl Brett G , Drezner Jonathan A 
Published: June 2014

BackgroundThis study compares the accuracy of cardiovascular screening in active adolescents and young adults using a standardised history, physical examination and resting 12-lead ECG.MethodsParticipants were prospectively screened using a standardised questionnaire based on the Pre-participation Physical Evaluation Monograph 4th Edition (PPE-4), physical examination and ECG interpreted using modern standards. Participants with abnormal findings had focused echocardiography and further evaluation. Primary outcomes included disorders associated with sudden cardiac arrest (SCA).ResultsFrom September 2010 to July 2011, 1339 participants underwent screening: age 13–24 (mean 16) years, 49% male, 68% Caucasian, 17% African-American and 1071 (80%) participating in organised sports. Abnormal history responses were reported on 916 (68%) questionnaires. After physician review, 495/916 (54%) participants with positive questionnaires were thought to have non-cardiac symptoms and/or a benign family history and did not warrant additional evaluation. Physical examination was abnormal in 124 (9.3%) participants, and 72 (5.4%) had ECG abnormalities. Echocardiograms were performed in 586 (44%) participants for abnormal history (31%), physical examination (8%) or ECG (5%). Five participants (0.4%) were identified with a disorder associated with SCA, all with ECG-detected Wolff-Parkinson-White. The false-positive rates for history, physical examination and ECG were 31.3%, 9.3% and 5%, respectively.ConclusionsA standardised history and physical examination using the PPE-4 yields a high false-positive rate in a young active population with limited sensitivity to identify those at risk for SCA. ECG screening has a low false-positive rate using modern interpretation standards and improves detection of primary electrical disease at risk of SCA.