bluuebunny/crossref_metadata_2025_split huggingface dataset:
Widespread Phytophthora infestations in European nurseries put forest, semi‐natural and horticultural ecosystems at high risk of Phytophthora diseases
Authors: Jung T. , Orlikowski L. , Henricot B. , Abad‐Campos P. , Aday A. G. , Aguín Casal O. , Bakonyi J. , Cacciola S. O. , Cech T. , Chavarriaga D. , Corcobado T. , Cravador A. , Decourcelle T. , Denton G. , Diamandis S. , Doğmuş‐Lehtijärvi H. T. , Franceschini A. , Ginetti B. , Green S. , Glavendekić M. , Hantula J. , Hartmann G. , Herrero M. , Ivic D. , Horta Jung M. , Lilja A. , Keca N. , Kramarets V. , Lyubenova A. , Machado H. , Magnano di San Lio G. , Mansilla Vázquez P. J. , Marçais B. , Matsiakh I. , Milenkovic I. , Moricca S. , Nagy Z. Á. , Nechwatal J. , Olsson C. , Oszako T. , Pane A. , Paplomatas E. J. , Pintos Varela C. , Prospero S. , Rial Martínez C. , Rigling D. , Robin C. , Rytkönen A. , Sánchez M. E. , Sanz Ros A. V. , Scanu B. , Schlenzig A. , Schumacher J. , Slavov S. , Solla A. , Sousa E. , Stenlid J. , Talgø V. , Tomic Z. , Tsopelas P. , Vannini A. , Vettraino A. M. , Wenneker M. , Woodward S. , Peréz‐Sierra A. 
Published: October 2015

SummaryAn analysis of incidence of Phytophthora spp. in 732 European nurseries producing forest transplants, larger specimen trees, landscape plants and ornamentals, plus 2525 areas in which trees and shrubs were planted, is presented based on work conducted by 38 research groups in 23 European countries between 1972 and 2013. Forty‐nine Phytophthora taxa were recorded in 670 nurseries (91.5%); within these nurseries, 1614 of 1992 nursery stands (81.0%) were infested, although most affected plants appeared healthy. In forest and landscape plantings, 56 Phytophthora taxa were recovered from 1667 of 2525 tested sites (66.0%). Affected plants frequently showed symptoms such as crown thinning, chlorosis and dieback caused by extensive fine root losses and/or collar rot. Many well‐known highly damaging host–Phytophthora combinations were frequently detected but 297 and 407 new Phytophthora–host associations were also observed in nurseries and plantings, respectively. On average, 1.3 Phytophthora species/taxa per infested nursery stand and planting site were isolated. At least 47 of the 68 Phytophthora species/taxa detected in nurseries and plantings were exotic species several of which are considered well established in both nurseries and plantings in Europe. Seven known Phytophthora species/taxa were found for the first time in Europe, while 10 taxa had not been previously recorded from nurseries or plantings; in addition, 5 taxa were first detections on woody plant species. Seven Phytophthora taxa were previously unknown to science. The reasons for these failures of plant biosecurity in Europe, implications for forest and semi‐natural ecosystems and possible ways to improve biosecurity are discussed.
Equine placenta – A clinician's perspective. Part 1: Normal placenta – Physiology and evaluation
Authors: Pozor M. 
Published: October 2015

SummaryA complex organ, the equine placenta is responsible for fetal nourishment, protection from external and internal insults, and the production and/or metabolisation of various hormones. The endometrial cups are unique structures of the equine placenta that are responsible for producing an essential hormone for equine pregnancy, equine chorionic gonadotropin. Since mares have epitheliochorial placentae, with 6 layers of tissue separating maternal from fetal circulation, almost the entire surface of the chorioallantois must be attached to the maternal endometrium in order to support a developing fetus adequately. The only avillous areas of the normal chorioallantois are: the cervical star, sites of the endometrial cups, areas facing oviductal papillae, folds overlying major allantoic vessels, and fetal footPADs (placental areas of degeneration). There are characteristic differences between the gravid and the nongravid horn of the chorioallantois. Allantoic vesicles, allantoic pouches, hippomanes, amniotic plaques and yolk sac remnants are normal features of the equine placenta. The clinician should thoroughly examine the entire placenta immediately after its expulsion. The most important aspect of this evaluation is to check for completeness of the chorioallantois, along with identifying any pathological lesions on the fetal membranes or the umbilical cord.
Conceptual feature generation for textual information using a conceptual network constructed from Wikipedia
Authors: Jadidinejad Amir H. , Mahmoudi Fariborz , Meybodi M. R. 
Published: October 2015

AbstractA proper semantic representation of textual information underlies many natural language processing tasks. In this paper, a novel semantic annotator is presented to generate conceptual features for text documents. A comprehensive conceptual network is automatically constructed with the aid of Wikipedia that has been represented as a Markov chain. Furthermore, semantic annotator gets a fragment of natural language text and initiates a random walk to generate conceptual features that represent topical semantic of the input text. The generated conceptual features are applicable to many natural language processing tasks where the input is textual information and the output is a decision based on its context. Consequently, the effectiveness of the generated features is evaluated in the task of document clustering and classification. Empirical results demonstrate that representing text using conceptual features and considering the relations between concepts can significantly improve not only the bag of words representation but also other state‐of‐the‐art approaches.
No decrease in f ratio (ratio of Festuca‐specific genome region to the whole genome) in maternally derived progeny of festulolium (Festuca pratensis × Lolium species) across generations
Authors: Kubota Akito , Akiyama Yukio , Fujimori Masahiro , Kiyoshi Takako 
Published: October 2015

AbstractFestulolium is an outbred, intergeneric hybrid between Lolium and Festuca species that might possess the agronomic advantages of both genera. As a result of DNA recombination, the chromosomes in festulolium are striped with DNA from the two genera. Using genomic in situ hybridization and image analysis techniques, we determined the genomic constitution of festulolium based on the ratio of Festuca‐specific genome regions to the whole genome (the “f ratio”). Some researchers have observed a drift in the genome balance in favor of Lolium over successive generations in amphiploid festulolium plants resulting from the cross between Lolium species and Festuca pratensis. In order to uncover the cause of the drift in favor of Lolium, we checked the f ratio drift between maternal plants and their progenies in three populations. One of the populations was a single cross progeny between parents of the backcrossed cultivar ‘Icarus,’ while the other two populations were isolated progenies of the amphiploid cultivar ‘Nakei 1.’ Because Lolium species produce more seeds than Festuca species, bulking of the seeds could cause the drift in favor of Lolium. In order to avoid this, progenies were maternally derived. In the present study, very few progeny plants had f ratios greater than 58%. The genomic drift in favor of Lolium in other studies might be due to the lack of very high f ratio plants. However, at least for one generation and with maternally derived progenies, Festuca chromosomal fragments did not disappear in either amphiploid or backcrossed festulolium cultivars, and the f ratio drift in favor of Lolium did not occur.
Immunohistochemical expression of matrix metalloproteinase‐1, matrix metalloproteinase‐2 and matrix metalloproteinase‐9, myofibroblasts and Ki‐67 in actinic cheilitis and lip squamous cell carcinoma
Authors: Bianco Bianca C. , Scotti Fernanda M. , Vieira Daniella S. C. , Biz Michelle T. , Castro Renata G. , Modolo Filipe 
Published: October 2015

SummaryMatrix metalloproteinases (MMPs), myofibroblasts (MFs) and epithelial proliferation have key roles in neoplastic progression. In this study immunoexpression of MMP‐1, MMP‐2 and MMP‐9, presence of MFs and the epithelial proliferation index were investigated in actinic cheilitis (AC), lip squamous cell carcinoma (LSCC) and mucocele (MUC). Thirty cases of AC, thirty cases of LSCC and twenty cases of MUC were selected for immunohistochemical investigation of the proteins MMP‐1, MMP‐2, MMP‐9, α‐smooth muscle actin (α‐SMA) and Ki‐67. The MMP‐1 expression in the epithelial component was higher in the AC than the MUC and LSCC. In the connective tissue, the expression was higher in the LSCC. MMP‐2 showed lower epithelial and stromal immunostaining in the LSCC when compared to the AC and MUC. The epithelial staining for MMP‐9 was higher in the AC when compared to the LSCC. However, in the connective tissue, the expression was lower in the AC compared to other lesions. The cell proliferation rate was increased in proportion to the severity of dysplasia in the AC, while in the LSCC it was higher in well‐differentiated lesions compared to moderately differentiated. There were no statistically significant differences in number of MFs present in the lesions studied. The results suggest that MMPs could affect the biological behaviour of ACs and LSCCs inasmuch as they could participate in the development and progression from premalignant lesions to malignant lesions.
Changes in calsequestrin, TNF‐α, TGF‐β and MyoD levels during the progression of skeletal muscle dystrophy in mdx mice: a comparative analysis of the quadriceps, diaphragm and intrinsic laryngeal muscles
Authors: Barros Maranhão Juliana , de Oliveira Moreira Drielen , Maurício Adriana Fogagnolo , de Carvalho Samara Camaçari , Ferretti Renato , Pereira Juliano Alves , Santo Neto Humberto , Marques Maria Julia 
Published: October 2015

SummaryIn Duchenne muscular dystrophy (DMD), the search for new biomarkers to follow the evolution of the disease is of fundamental importance in the light of the evolving gene and pharmacological therapies. In addition to the lack of dystrophin, secondary events including changes in calcium levels, inflammation and fibrosis greatly contribute to DMD progression and the molecules involved in these events may represent potential biomarkers. In this study, we performed a comparative evaluation of the progression of dystrophy within muscles that are differently affected by dystrophy (diaphragm; DIA and quadriceps; QDR) or spared (intrinsic laryngeal muscles) using the mdx mice model of DMD. We assessed muscle levels of calsequestrin (calcium‐related protein), tumour necrosis factor (TNF‐α; pro‐inflammatory cytokine), tumour growth factor (TGF‐β; pro‐fibrotic factor) and MyoD (muscle proliferation) vs. histopathology at early (1 and 4 months of age) and late (9 months of age) stages of dystrophy. Fibrosis was the primary feature in the DIA of mdx mice (9 months: 32% fibrosis), which was greater than in the QDR (9 months: 0.6% fibrosis). Muscle regeneration was the primary feature in the QDR (9 months: 90% of centrally nucleated fibres areas vs. 33% in the DIA). The QDR expressed higher levels of calsequestrin than the DIA. Laryngeal muscles showed normal levels of TNF‐α, TGF‐β and MyoD. A positive correlation between histopathology and cytokine levels was observed only in the diaphragm, suggesting that TNF‐α and TGF‐β serve as markers of dystrophy primarily for the diaphragm.
Effects of the association of diabetes and pulmonary emphysema on cardiac structure and function in rats
Authors: Di Petta Antonio , Simas Rafael , Ferreira Clebson L. , Capelozzi Vera L. , Salemi Vera M. C. , Moreira Luiz F. P. , Sannomiya Paulina 
Published: October 2015

SummaryChronic obstructive pulmonary disease is often associated with chronic comorbid conditions of cardiovascular disease, diabetes mellitus and hypertension. This study aimed to investigate the effects of the association of diabetes and pulmonary emphysema on cardiac structure and function in rats. Wistar rats were divided into control non‐diabetic instilled with saline (CS) or elastase (CE), diabetic instilled with saline (DS) or elastase (DE), DE treated with insulin (DEI) groups and echocardiographic measurements, morphometric analyses of the heart and lungs, and survival analysis conducted 50 days after instillation. Diabetes mellitus was induced [alloxan, 42 mg/kg, intravenously (iv)] 10 days before the induction of emphysema (elastase, 0.25 IU/100 g). Rats were treated with NPH insulin (4 IU before elastase plus 2 IU/day, 50 days). Both CE and DE exhibited similar increases in mean alveolar diameter, which are positively correlated with increases in right ventricular (RV) wall thickness (P = 0.0022), cavity area (P = 0.0001) and cardiomyocyte thickness (P = 0.0001). Diabetic saline group demonstrated a reduction in left ventricular (LV) wall, interventricular (IV) septum, cardiomyocyte thickness and an increase in cavity area, associated with a reduction in LV fractional shortening (P < 0.05), and an increase in LViv relaxation time (P < 0.05). Survival rate decreased from 80% in DS group to 40% in DE group. In conclusion, alloxan diabetes did not affect RV hypertrophy secondary to chronic emphysema, even in the presence of insulin. Diabetes per se induced left ventricular dysfunction, which was less evident in the presence of RV hypertrophy. Survival rate was substantially reduced as a consequence, at least in part, of the coexistence of RV hypertrophy and diabetic cardiomyopathy.
Antioxidant activities of Thai pigmented rice cultivars and application in sunflower oil
Authors: Chinprahast Ninnart , Tungsomboon Teerapat , Nagao Patlapa 
Published: October 2015

SummaryAn analytical comparison for active chemical compounds and their antioxidant activities between three representative pigmented Thai rice cultivars was performed. It was revealed that the darkest purple‐red cultivar (Kum Doi Musur, KDM) contained the highest total phenolic content of 1164.6 mg gallic acid equivalent per100 g (dry basis, db) and the highest total monomeric anthocyanin content of 381.7 mg per100 g (db), when compared with the purple cultivar (Riceberry) and the red cultivar (Sungyod). Its 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging activity and ferric reducing antioxidant power were also the highest. When applied in sunflower oil, the KDM crude extract was efficiently capable of retarding lipid peroxidation as assessed by peroxide value, p‐anisidine value, totox value and the Rancimat method. Results show this specific pigmented rice to be a potent antioxidant for stabilisation of sunflower oil.
Effects of TiO2–UVC photocatalysis and thermal pasteurisation on microbial inactivation and quality characteristics of the Korean rice‐and‐malt drink sikhye
Authors: Shahbaz Hafiz M. , Kim Sanghun , Hong Jungyeon , Kim Jeong Un , Lee Dong‐Un , Ghafoor Kashif , Park Jiyong 
Published: October 2015

SummarySikhye is a popular Korean rice‐and‐malt drink. This drink is prepared using a specific traditional process and has short shelf life. Commercial processing using ultra‐high temperature affects characteristic colour and flavour attributes and disturbs the traditional method of sikhye preparation. The objective of this study was to compare the effect of TiO2–UVC photocatalysis (TUVP) nonthermal processing with heat‐pasteurised and untreated sikhye samples during storage at 4 and 10 °C. TUVP and heat treatments were both sufficient to inhibit regrowth of total aerobic bacteria and coliform during storage. Hunter colour parameter values showed that the colour of sikhye was less affected by TUVP treatment compared to the heat treatment. Higher sensory scores for flavour, taste and overall preference were attained for low‐dosage TUVP‐treated (0.24 J cm−2) samples than for heat‐treated samples but without a significant difference. TUVP treatment effectively controlled the microbial growth during storage at 4 °C for more than 18 days which was reported a serious microbiological safety issue. In summary, TUVP showed its suitability for commercialisation possibilities of fresh sikhye drink by inactivating the main micro‐organisms and thus increasing significantly its shelf life with minimum impact on quality characteristics.
Clinico‐microbiological study of Pseudomonas aeruginosa in wound infections and the detection of metallo‐β‐lactamase production
Authors: Bangera Divya , Shenoy Suchitra M , Saldanha Dominic RM 
Published: October 2015

AbstractPseudomonas aeruginosa is a common opportunistic pathogen of humans among the Gram‐negative bacilli. Clinically, it is associated with nosocomial infections like burns and surgical‐site wound infections and remains a major health concern, especially among critically ill and immunocompromised patients. This is a prospective laboratory‐based 2 year study conducted to isolate P. aeruginosa from wound specimens and the antimicrobial susceptibility pattern with reference to metallo‐β‐lactamase (MBL) production. Two hundred and twenty‐four samples of P. aeruginosa isolated from wound specimens were included in the study. Antimicrobial susceptibility was done as per Clinical Laboratory Standard Institute (CLSI) guidelines. MBL‐producing P. aeruginosa was detected using the EDTA disk diffusion synergy test. Statistical analysis was done using the SPSS 11 package (SPSS Inc., Chicago, IL). Out of the 224 P. aeruginosa isolates, 100% were susceptible to polymyxin B and colistin, 92·8% were sensitive to imipenem, 38% showed resistance to gentamicin followed by ceftazidime (31·69%) and meropenem (33·03). Sixteen (7·14%) isolates showed MBL production. Infection caused by drug‐resistant P. aeruginosa is important to identify as it poses a therapeutic problem and is also a serious concern for infection control management. The acquired resistance genes can be horizontally transferred to other pathogens or commensals if aseptic procedures are not followed.
Employed Carers’ Empathy Towards People with Intellectual Disabilities: The Development of a New Measure and Some Initial Theory
Authors: Collins Kirsten , Gratton Caroline , Heneage Celia , Dagnan Dave 
Published: October 2015

BackgroundThis study aimed to develop a self‐report measure of paid caregivers’ empathy towards people with intellectual disabilities.Materials and MethodsFollowing questionnaire develop‐ment, 194 staff working in services for people with intellectual disabilities completed self‐report questionnaires, including the new empathy measure. The measure's factor structure and psychometric properties were investigated.ResultsA three factor solution suggested two key processes in empathizing: experiencing commonality between one's own and people with intellectual disabilities’ psychological experiences and efforts to attune to their internal worlds. The final factor represented whether carers find it challenging to empathize. Correlations with beliefs about the self and others in caregiving relationships provided initial evidence of validity, although further investigation is needed.ConclusionsThe most salient processes in empathizing with people with intellectual disabilities may be different from empathy in other contexts. Establishing determinants of carer empathy may facilitate the development of psychological interventions to promote and enhance this important quality.
Amniotic mesenchymal cells from pre‐eclamptic placentae maintain immunomodulatory features as healthy controls
Authors: Pianta Stefano , Magatti Marta , Vertua Elsa , Bonassi Signoroni Patrizia , Muradore Ivan , Nuzzo Anna Maria , Rolfo Alessandro , Silini Antonietta , Quaglia Federico , Todros Tullia , Parolini Ornella 
Published: October 2015

AbstractPre‐eclampsia (PE) is one of the most severe syndromes in human pregnancy, and the underlying mechanisms of PE have yet to be determined. Pre‐eclampsia is characterized by the alteration of the immune system's activation status, an increase in inflammatory Th1/Th17/APC cells, and a decrease in Th2/Treg subsets/cytokines. Moreover, inflammatory infiltrates have been detected in the amniotic membranes of pre‐eclamptic placentae, and to this date limited data are available regarding the role of amniotic membrane cells in PE. Interestingly, we and others have previously shown that human amniotic mesenchymal stromal cells (hAMSC) possess anti‐inflammatory properties towards almost all immune cells described to be altered in PE. In this study we investigated whether the immunomodulatory properties of hAMSC were altered in PE. We performed a comprehensive study of cell phenotype and investigated the in vitro immunomodulatory properties of hAMSC isolated from pre‐eclamptic pregnancies (PE‐hAMSC), comparing them to hAMSC from normal pregnancies (N‐hAMSC). We demonstrate that PE‐hAMSC inhibit CD4/CD8 T‐cell proliferation, suppress Th1/Th2/Th17 polarization, induce Treg and block dendritic cells and M1 differentiation switching them to M2 cells. Notably, PE‐hAMSC generated a more prominent induction of Treg and higher suppression of interferon‐γ when compared to N‐hAMSC, and this was associated with higher transforming growth factor‐β1 secretion and PD‐L2/PD‐L1 expression in PE‐hAMSC. In conclusion, for the first time we demonstrate that there is no intrinsic impairment of the immunomodulatory features of PE‐hAMSC. Our results suggest that amniotic mesenchymal stromal cells do not contribute to the disease, but conversely, could participate in offsetting the inflammatory environment which characterizes PE.
Olfactory cues explain differential attraction of the striped rice stem borer to different varieties of rice plant
Authors: Ghaninia M. , Amooghli Tabari M. 
Published: October 2015

AbstractIn Iranian rice fields, different varieties of rice are cultivated which are differentially impacted by females of the rice stem borer,Chilo suppressalis. To elucidate the role odours may play in their host plant finding behaviour, femaleCh. suppressaliswere exposed to four varieties of rice plants and their volatiles in a four‐arm olfactometer. In whole plant tests,Ch. suppressaliswere significantly attracted to the variety previously characterized as most susceptible, least attracted to one characterized as semisusceptible, and showed no attraction to those varieties characterized as semi‐ and highly resistant. Tests using headspace volatile extracts yielded similar results in the case of the most susceptible variety, but showed no attraction to the semisusceptible and highly resistant varieties, and low attraction to the semiresistant variety. Subsequent analysis of the volatile composition identified a panel of 27 components, some of which were either unique to, or abundantly present in, particular varieties, and may explain the observed variation in their attractiveness. Our findings show that rice plant volatiles can play a role in the host selection behaviour of this pest species, and we suggest compounds which may be important to this process and the future application of volatiles in rice pest management programs.