bluuebunny/crossref_metadata_2025_split huggingface dataset:
Superficial layer pyramidal cells communicate heterogeneously between multiple functional domains of cat primary visual cortex
Authors: Martin Kevan A. C. , Roth Stephan , Rusch Elisha S. 
Published: October 2014

AbstractThe axons of pyramidal neurons in the superficial layers of the neocortex of higher mammals form lateral networks of discrete clusters of synaptic boutons. In primary visual cortex the clusters are reported to link domains that share the same orientation preferences, but how individual neurons contribute to this network is unknown. Here we performed optical imaging to record the intrinsic signal, which is an indirect measure of neuronal firing, and determined the global map of orientation preferences in the cat primary visual system. In the same experiment, single cells were recorded and labelled intracellularly. We found that individual axons arborise within the retinotopic representation of the classical receptive field, but their bouton clusters were not aligned along their preferred axis of orientation along the retinotopic map. Axon clusters formed in a variety of different orientation domains, not just the like-orientation domains. This topography and heterogeneity of single-cell connectivity provides circuits for normalization and context-dependent feature processing of visual scenes.
miR-21 improves the neurological outcome after traumatic brain injury in rats
Authors: Ge Xin-Tong , Lei Ping , Wang Hai-Chen , Zhang An-Ling , Han Zhao-Li , Chen Xin , Li Sheng-Hui , Jiang Rong-Cai , Kang Chun-Sheng , Zhang Jian-Ning 
Published: October 2014

AbstractThe expression levels of microRNAs (miRNAs) including miR-21, have been reported to change in response to traumatic brain injury (TBI), suggesting that they may influence the pathophysiological process in brain injury. To analyze the potential effect of miR-21 on neurological function after TBI, we employed the fluid percussion injury rat model and manipulated the expression level of miR-21 in brain using intracerebroventricular infusion of miR-21 agomir or antagomir. We found that upregulation of miR-21 level in brain conferred a better neurological outcome after TBI by improving long-term neurological function, alleviating brain edema and decreasing lesion volume. To further investigate the mechanism underlying this protective effect, we evaluated the impact of miR-21 on apoptosis and angiogenesis in brain after TBI. We found that miR-21 inhibited apoptosis and promoted angiogenesis through regulating the expression of apoptosis- and angiogenesis-related molecules. In addition, the expression of PTEN, a miR-21 target gene, was inhibited and Akt signaling was activated in the procedure. Taken together, these data indicate that miR-21 could be a potential therapeutic target for interventions after TBI.
Trophic interactions induce spatial self-organization of microbial consortia on rough surfaces
Authors: Wang Gang , Or Dani 
Published: October 2014

AbstractThe spatial context of microbial interactions common in natural systems is largely absent in traditional pure culture-based microbiology. The understanding of how interdependent microbial communities assemble and coexist in limited spatial domains remains sketchy. A mechanistic model of cell-level interactions among multispecies microbial populations grown on hydrated rough surfaces facilitated systematic evaluation of how trophic dependencies shape spatial self-organization of microbial consortia in complex diffusion fields. The emerging patterns were persistent irrespective of initial conditions and resilient to spatial and temporal perturbations. Surprisingly, the hydration conditions conducive for self-assembly are extremely narrow and last only while microbial cells remain motile within thin aqueous films. The resulting self-organized microbial consortia patterns could represent optimal ecological templates for the architecture that underlie sessile microbial colonies on natural surfaces. Understanding microbial spatial self-organization offers new insights into mechanisms that sustain small-scale soil microbial diversity; and may guide the engineering of functional artificial microbial consortia.
Atomic Diffusion within Individual Gold Nanocrystal
Authors: Xiong Gang , Clark Jesse N. , Nicklin Chris , Rawle Jonathan , Robinson Ian K. 
Published: October 2014

AbstractDue to their excess surface free energy and structural instabilities, nanoparticles exhibit interesting physical and chemical properties. There has been an ever-growing interest in investigating these properties, driven by the desire to further miniaturize electronic devices, develop new functional materials and catalysts. Here, the intriguing question of how diffusion evolves in a single nanoparticle is investigated by measuring the spatial and temporal variations of the diffracted coherent X-ray intensity during copper diffusion into a gold nanocrystal. Dislocation loops formed from the insertion of single layer of extra atoms between neighbouring gold host lattice planes are detected. Au-Cu alloy channels are found to penetrate the nanocrystal due to the differential diffusion rate along different directions. With the advent of higher brilliance sources and free-electron-lasers, Bragg Coherent X-ray Diffraction Imaging can play an important role in unveiling atomic behaviours in three dimensions for nanomaterials during various fundamental processes.
BriGuard: a lightweight indoor intrusion detection system based on infrared light spot displacement
Authors: Liu Leibo , Zhang Weilong , Deng Chenchen , Yin Shouyi , Wei Shaojun 
Published: October 2014

Indoor intrusion detection systems are widely implemented in many special departments and environments. Owing to the special target market, several specific features of such systems are required including high accuracy, high performance, high concealment and ease of deployment. In this study, the authors proposed an infrared light spot displacement‐based surveillance system for indoor intrusion detection. This system, named BriGuard, shares a similar idea with radar. A 940 nm infrared light beam goes through an optical diffraction grating to generate light spot matrix imagery in the view of a complementary metal oxide semiconductor image sensor. The coordinates of the light spots are extracted and recorded. When someone enters the monitored area, the imaging plane changes and leads to a variation of the pre‐recorded light spots coordinates. The light beam is modulated in order to be distinguished from the light in the environment. The lightweight algorithms require little computation power and data storage. BriGuard achieves a nearly zero false detection rate while covering a distance up to 10 m. It can process 30 full HD (1920 × 1080) frames per second. More importantly, because of unnoticeable infrared lights and very small form factor, the whole detection equipment is very hard to observe.
Deep Infection after Total Hip Replacement: A Method for National Incidence Surveillance
Authors: Lindgren Viktor , Gordon Max , Wretenberg Per , Kärrholm Johan , Garellick Göran 
Published: October 2014

Objective.The purpose of this study was to estimate the incidence of deep periprosthetic joint infections (PJIs) after primary total hip replacement (THR) in Sweden prior to the introduction of a national initiative to reduce these infections.Design.Prospective open cohort study with 2 years follow-up of each subject.Setting.All THR-performing clinics in Sweden.Methods.All patients registered for a primary THR in the Swedish Hip Arthroplasty Register between July 1, 2005, and December 31, 2008, were selected for the study (45,531 patients with 49,219 THRs) and were matched with the Swedish Prescribed Drug Register. All patients with a minimum of 4 weeks of continuous outpatient antibiotic treatment within 2 years after their primary THR (1,989 patients with 2,219 THRs) were selected for a medical records review, and the number of cases with PJI was determined.Results.The cumulative incidence of PJI within 2 years after primary THR was 0.9% (95% confidence interval, 0.85–1.02; n = 443), and 405 of these had been reoperated. The incidence rate of PJI for the first 3 months was 5 per 10,000 THR-weeks and, thereafter, 0.3 per 10,000 THR-weeks. Staphylococcus aureus and coagulase-negative staphylococci were the most common bacteria isolated.Conclusions.This study describes a new method of national postoperative infection surveillance. The incidence is similar to previous smaller reports and is useful for monitoring changes over time to evaluate the national initiative to reduce infections.
A systematic survey of an intragenic epistatic landscape
Authors: Bank Claudia , Hietpas Ryan T. , Jensen Jeffrey D. , Bolon Daniel N.A. 
Published: October 2014

ABSTRACTMutations are the source of evolutionary variation. The interactions of multiple mutations can have important effects on fitness and evolutionary trajectories. We have recently described the distribution of fitness effects of all single mutations for a nine amino acid region of yeast Hsp90 (Hsp82) implicated in substrate binding. Here, we report and discuss the distribution of intragenic epistatic effects within this region in seven Hsp90 point mutant backgrounds of neutral to slightly deleterious effect, resulting in an analysis of more than 1000 double-mutants. We find negative epistasis between substitutions to be common, and positive epistasis to be rare – resulting in a pattern that indicates a drastic change in the distribution of fitness effects one step away from the wild type. This can be well explained by a concave relationship between phenotype and genotype (i.e., a concave shape of the local fitness landscape), suggesting mutational robustness intrinsic to the local sequence space. Structural analyses indicate that, in this region, epistatic effects are most pronounced when a solvent-inaccessible position is involved in the interaction. In contrast, all 18 observations of positive epistasis involved at least one mutation at a solvent-exposed position. By combining the analysis of evolutionary and biophysical properties of an epistatic landscape, these results contribute to a more detailed understanding of the complexity of protein evolution.
Adapting smart specialisation to a micro-economy – the case of Malta
Authors: Georghiou Luke , Elvira Uyarra and , Saliba Scerri Ramona , Castillo Nadine , Cassingena Harper Jennifer 
Published: October 2014

Purpose
– The purpose of this paper is to set out the process by which a smart specialisation strategy was developed for a small, peripheral economy in the European Union, the Republic of Malta. It assesses the applicability of the approach in the context of a micro-economy with an industrial structure based on a small number of foreign direct investments and a predominance of micro-enterprises.

Design/methodology/approach
– The paper follows an action research approach by presenting as a case study the process by which the strategy for Malta was assessed and developed through successive rounds of engagement with business and other actors with the application of scenarios and other prioritisation approaches to facilitate its development. An initial consultation with 20 public sector and representative organisations was followed by a general business workshop and 21 sectoral focus groups.

Findings
– Lack of critical mass can be mitigated by maximising the generic use of available skills and competences. Given the higher vulnerability to external shock in micro-economies, strategies need to have a high degree of flexibility and adaptability. Greater internationalisation provides the main response to peripherality.

Practical implications
– The approach can be applied more generally for micro-economies and in some aspects to other countries or regions lacking critical mass in research and innovation assets or facing peripherality.

Originality/value
– The smart specialisation approach had not been applied in these circumstances and hence the findings allowed the concept to be extended and adapted to deal with the issues raised.
Smart specialisation in European regions: issues of strategy, institutions and implementation
Authors: McCann Philip , Ortega-Argilés Raquel 
Published: October 2014

Purpose
– The purpose of this paper is to show that the approaches to smart specialisation being adopted in different European Union (EU) regions are likely to be heavily shaped by the institutional and governance context, as well as the regional economic specifics. Along with the specific regional economic characteristics, these institutional variations mean that there is no single smart specialisation template or blueprint which can be transplanted onto every region. Rather, regions have to work within their own governance frameworks to find their best solutions.

Design/methodology/approach
– As evidence of this, the authors analyse the possibilities and challenges faced by four different sets of regional examples in the UK, the Netherlands, Belgium and Spain. Using OECD, EU and other official national documents and publications, the authors are able to explain the ways in which the governance set-ups vary enormously across these different arenas although they do share some certain common features with the other examples on a case-by-case basis.

Findings
– The policy architecture within which the smart specialisation agenda will be operating is very different in each national or regional case. As such, in addition to the regional economic specifics, the smart specialisation challenges faced by different regions are likely to differ significantly due to governance issues as well as variations in the regional economic conditions. This is because the possibilities for different regional actions depend heavily on the governance relationship between the regional and the local governance remits.

Research limitations/implications
– The argument presented here are necessarily in part speculative in that while they are based on a regional systems-of-innovation conceptual framework which links institutions, innovation and regional development, the actual smart specialisation implementation processes are still in their infancy, so that the actual outcomes remain to be seen in the long run.

Practical implications
– The analysis here helps to situate smart specialisation discussions in the national-regional institutional and governance context. This also serves to frame how smart specialisation priority-setting processes are likely to be undertaken and helps to consider how such activities may play out in other regions with different institutional settings.

Originality/value
– This is one of the few papers that explicitly examine specialisation issues in a governance and institutional setting. In reality, the success or otherwise of smart specialisation agenda will be heavily shaped by how the governance and institutional issues are addressed. Good analysis and data gathering is essential, but good governance for policy design, monitoring and evaluation can potentially also provide a crucial advantage to smart specialisation actions. In contrast, poor governance may undermine good smart specialisation intentions and analyses.
Guest editorial on research and innovation strategies for smart specialisation in Europe
Authors: Landabaso Mikel 
Published: October 2014

Purpose
– This Special Issue of the European Journal of Innovation Management sheds new light on the burning issue of Research and Innovation Strategies for Smart Specialisation (RIS3), both in terms of their policy formulation and their practical implementation in the field. This new policy approach refers to the process of priority setting in national and regional research and innovation strategies in order to build “place-based” competitive advantages and help regions and countries develop an innovation-driven economic transformation agenda. The paper aims to discuss these issues.

Design/methodology/approach
– This is an important topic both in the current debate about a new industrial policy for Europe and as a policy option for a successful crisis exit strategy led by public investments in the real economy. Moreover, smart specialisation is promoted by the European Commission as an ex ante conditionality for all regions in Europe to receive European Structural and Investment Funds in the field of innovation. Thus, it has become a pre-requisite for accessing fresh funds for investing in badly needed innovation-driven productivity growth throughout the European Union (EU).

Findings
– The six papers in this Special Issue are the fruit of ground-breaking research and policy testing by nearly 20 leading academics and policy makers throughout the EU. They explore the early smart specialisation concept and its further developments, examine the methodological tools at its disposal and advance specific policy proposals and governance considerations based on actual experimentation in the field.

Originality/value
– All these make the present Special Issue of the European Journal of Innovation Management an important research milestone. This Special Issue is the fruit of a call towards the European academic and research community to help shaping and advancing the smart specialisation concept and thus contribute to better position regions and countries in the global economy through innovation-driven policies.
Workforce ageing and the training propensity of Italian firms
Authors: Guerrazzi Marco 
Published: October 2014

Purpose– The purpose of this paper is to take into consideration the propensity to offer vocational training of a large sample of Italian private firms by retrieving cross-sectional data from INDACO (2009).Design/methodology/approach– Estimating a probit model, the author assesses how the age and the gender composition of the employed workforce, as well as a set of relevant corporate characteristics, such as size, sector, geographical location, innovation strategies, R&D investments and use of social safety valves, are linked to the willingness of firms to supply on-the-job training.Findings– First, as far as the average age of the whole employed workforce is concerned, it was found that the propensity of surveyed firms toward training provision follows an inverted U-shaped pattern. Furthermore, it was shown that larger firms have a higher training propensity with respect to small firms, and the same attitude holds for productive units that adopted innovation strategies and/or invested in R&D projects. By contrast, it was found that the propensity to support training activities is negatively correlated to the percentage of employed women and the use social valves.Research limitations/implications– The sample of business units taken into consideration is quite large, but it has some biases toward larger and manufacturing firms. Moreover, the cross-sectional perspective of the analysis does not allow implementation of the finer identification procedures that can be applied with panel data. Furthermore, the lack of employer – employee linked data does not allow to fully address the issue of compliance to training activities.Social implications– From a policy point of view, the results shown throughout the paper suggest some broad guidelines. First, especially in small firms, vocational training for young and older workers should be somehow stimulated. Moreover, as far as mature employees are concerned, those interventions should be framed in an active ageing perspective. Subsidies and targeted job placement programmes are often claimed as being the most appropriate ways to improve the underprivileged position of older workers. However, continuous learning during the whole working life still appears as the most effective device to reduce the employment disadvantages in the older years.Originality/value– While there are a number of papers that study the age patterns of training participation by using workers’ data retrieved from personnel and/or labour force surveys, this work is the first attempt to provide a probabilistic assessment of the decisions of Italian firms regarding training provision by taking into account the ageing perspectives of the incumbent workforce.
Unpacking the relationship between organizational culture and excellent leadership in public relations
Authors: Meng Juan 
Published: October 2014

Purpose– By looking into the relationship between organizational culture and excellent leadership in public relations, the purpose of this paper is bifold: first, how and to what extent organizational culture can affect leadership effectiveness in communication management; and second, what possible impact excellent leadership in public relations may generate to reshape organizational culture in a way to further support the value of public relations.Design/methodology/approach– An online survey was distributed to a randomly selected group of 1,000 senior public relations executives in the USA by using the PR executive database of Heyman Associates, a PR executive search firm in New York City. A final sample of 222 senior public relations executives was recruited. This group of respondents mirrored the characteristics of senior executives in the public relations profession in the USA.Findings– The results identified the reciprocal relationship between organizational culture and excellent leadership in public relations. The testing of a non-recursive structural model confirmed that organizational culture generates a direct, positive effect on the achievement of excellent leadership in public relations. More importantly, excellent leadership in public relations also influences organizational culture by reshaping it in a favorable way to support public relations efforts in the organization.Originality/value– There is insufficient empirical research linking public relations leadership and organizational culture, although it is agreed these two institutional conditions are critical. Thus, this study unpacked the independent and dependent roles of organizational culture in influencing leadership effectiveness in public relations, which may generate implications for both research and practice purposes.
An attributional analysis of corporate reporting in crisis situations
Authors: Tennert Falk 
Published: October 2014

Purpose
– The purpose of this paper is to use an attributional approach to examine press coverage in Germany dealing with Toyota’s 2010 global product recall due to purportedly defective brakes. The research focuses on the attributions of cause and responsibility and, thereby, the practices of media-brokered selection and interpretation of events.

Design/methodology/approach
– The methodology used is a quantitative content analysis of selected German print media. Corporate reporting is analysed with the help of attribution theory approaches from the field of psychology, which, when applied to public relations themes, thereby enables the identification of latent and manifest risk factors that emerge from the perceived responsibility of the media.

Findings
– Causal attributions are an essential aspect of coverage in acute crisis situations. The key findings show a dominance of internal attributions of responsibility in which the media interprets the crisis as self inflicted and ascribes a high level of fault on the company. Exonerating attributions according to a self-serving bias find little resonance in the coverage. The responsibility attributed to Toyota by the media coverage to a sustained damage to the company’s reputation.

Originality/value
– The study demonstrates that attribution theory can be productively applied to questions of communication management. This approach enables an analysis of attribution discourse as well as the potential long-term effects on the company’s reputation. Thus, the original value of this study lies in the psychological foundation of organisational risk and opportunity.