bluuebunny/crossref_metadata_2025_split huggingface dataset:
Articular Chondroprogenitor Cells Maintain Chondrogenic Potential but Fail to Form a Functional Matrix When Implanted Into Muscles of SCID Mice
Authors: Marcus Paula , De Bari Cosimo , Dell’Accio Francesco , Archer Charles W. 
Published: July 2014

Objective Articular cartilage is a complex tissue comprising phenotypically distinct zones. Research has identified the presence of a progenitor cell population in the surface zone of immature articular cartilage. The aim of the present study was to determine the in vivo plasticity of articular cartilage progenitor. Design Chondropogenitor cells were isolated from bovine metacarpalphalangeal joints by differential adhesion to fibronectin. Cells were labeled with PKH26 and injected into the thigh muscle of severe-combined immunodeficient (SCID) mice. After 2 weeks, the muscles were dissected and cryosectioned. Sections were stained with safranin O and labeled for sox9 and collagen type II. Polymerase chain reaction analysis was carried out to determine plasticity for a number of tissue-specific markers. Full-depth chondrocytes acted as a control. Results Fluorescent PKH26 labeled cells were detected after 2 weeks in all samples analyzed. A cartilage pellet was present after injection of freshly isolated chondrocytes. After injection with clonal and enriched populations of chondroprogenitors, no distinct pellet was detected, but diffuse cartilage nodules were found with regions of safranin O staining and Sox9. Low levels of collagen type II were also detected. Polymerase chain reaction analysis identified the presence of the endothelial cell marker PECAM-1 in one clonal cell line, demonstrating phenotypic plasticity into the phenotype of the surrounding host tissues. Conclusions The bovine articular cartilage progenitor cells were able to survive in vivo postimplantation, but failed to create a robust cartilage pellet, despite expressing sox9 and type II collagen. This suggests the cells require further signals for chondrogenic differentiation.
Common genes underlying asthma and COPD? Genome-wide analysis on the Dutch hypothesis
Authors: Smolonska Joanna , Koppelman Gerard H. , Wijmenga Cisca , Vonk Judith M. , Zanen Pieter , Bruinenberg Marcel , Curjuric Ivan , Imboden Medea , Thun Gian-Andri , Franke Lude , Probst-Hensch Nicole M. , Nürnberg Peter , Riemersma Roland A. , van Schayck Constant P. , Loth Daan W. , Brusselle Guy G. , Stricker Bruno H. , Hofman Albert , Uitterlinden André G. , Lahousse Lies , London Stephanie J. , Loehr Laura R. , Manichaikul Ani , Barr R. Graham , Donohue Kathleen M. , Rich Stephen S. , Pare Peter , Bossé Yohan , Hao Ke , van den Berge Maarten , Groen Harry J.M. , Lammers Jan-Willem J. , Mali Willem , Boezen H. Marike , Postma Dirkje S. 
Published: July 2014

Asthma and chronic obstructive pulmonary disease (COPD) are thought to share a genetic background (“Dutch hypothesis”).We investigated whether asthma and COPD have common underlying genetic factors, performing genome-wide association studies for both asthma and COPD and combining the results in meta-analyses.Three loci showed potential involvement in both diseases: chr2p24.3, chr5q23.1 and chr13q14.2, containingDDX1,COMMD10(both participating in the nuclear factor (NF) κβ pathway) andGNG5P5, respectively. Single nucleotide polymorphisms (SNPs) rs9534578 inGNG5P5reached genome-wide significance after first replication phase (p=9.96×10−9). The second replication phase, in seven independent cohorts, provided no significant replication. Expression quantitative trait loci (eQTL) analysis in blood cells and lung tissue on the top 20 associated SNPs identified two SNPs inCOMMD10that influenced gene expression.Inflammatory processes differ in asthma and COPD and are mediated by NF-κβ, which could be driven by the same underlying genes,COMMD10andDDX1. None of the SNPs reached genome-wide significance. Our eQTL studies support a functional role for twoCOMMD10SNPs, since they influence gene expression in both blood cells and lung tissue. Our findings suggest that there is either no common genetic component in asthma and COPD or, alternatively, different environmental factors,e.g.lifestyle and occupation in different countries and continents, which may have obscured the genetic common contribution.
The COPD assessment test: a systematic review
Authors: Gupta Nisha , Pinto Lancelot M. , Morogan Andreea , Bourbeau Jean 
Published: July 2014

The COPD assessment test (CAT) is a self-administered questionnaire that measures health-related quality of life. We aimed to systematically evaluate the literature for reliability, validity, responsiveness and minimum clinically important difference (MCID) of the CAT.Multiple databases were searched for studies analysing the psychometric properties of the CAT in adults with chronic obstructive pulmonary disease. Two reviewers independently screened, selected and extracted data, and assessed methodological quality of relevant studies using the COSMIN checklist.From 792 records identified, 36 studies were included. The number of participants ranged from 45 to 6469, mean age from 56 to 73 years, and mean forced expiratory volume in 1 s from 39% to 98% predicted. Internal consistency (reliability) was 0.85–0.98, and test–retest reliability was 0.80–0.96. Convergent and longitudinal validity using Pearson’s correlation coefficient were: SGRQ-C 0.69–0.82 and 0.63, CCQ 0.68–0.78 and 0.60, and mMRC 0.29–0.61 and 0.20, respectively. Scores differed with GOLD stages, exacerbation and mMRC grades. Mean scores decreased with pulmonary rehabilitation (2.2–3 units) and increased at exacerbation onset (4.7 units). Only one study with adequate methodology reported an MCID of 2 units and 3.3–3.8 units using the anchor-based approach and distribution-based approach, respectively. Most studies had fair methodological quality.We conclude that the studies support the reliability and validity of the CAT and that the tool is responsive to interventions, although the MCID remains debatable.
Nitric oxide-releasing porous silicon nanoparticles
Authors: Kafshgari Morteza Hasanzadeh , Cavallaro Alex , Delalat Bahman , Harding Frances J , McInnes Steven JP , Mäkilä Ermei , Salonen Jarno , Vasilev Krasimir , Voelcker Nicolas H 
Published: July 2014

AbstractIn this study, the ability of porous silicon nanoparticles (PSi NPs) to entrap and deliver nitric oxide (NO) as an effective antibacterial agent is tested against different Gram-positive and Gram-negative bacteria. NO was entrapped inside PSi NPs functionalized by means of the thermal hydrocarbonization (THC) process. Subsequent reduction of nitrite in the presence ofd-glucose led to the production of large NO payloads without reducing the biocompatibility of the PSi NPs with mammalian cells. The resulting PSi NPs demonstrated sustained release of NO and showed remarkable antibacterial efficiency and anti-biofilm-forming properties. These results will set the stage to develop antimicrobial nanoparticle formulations for applications in chronic wound treatment.
Indium droplet formation in InGaN thin films with single and double heterojunctions prepared by MOCVD
Authors: Chen Yung-Sheng , Liao Che-Hao , Kuo Chie-Tong , Tsiang Raymond Chien-Chao , Wang Hsiang-Chen 
Published: July 2014

Abstract
Indium gallium nitride (InGaN) samples with single heterojunction (SH) and double heterojunction (DH) were prepared using metal-organic chemical vapor deposition. SH has a layer of InGaN thin film (thicknesses, 25, 50, 100, and 200 nm) grown on an uGaN film (thickness, 2 μm). The DH samples are distinguished by DH uGaN film (thickness, 120 nm) grown on the InGaN layer. Reciprocal space mapping measurements reveal that the DH samples are fully strained with different thicknesses, whereas the strain in the SH samples are significantly relaxed with the increasing thickness of the InGaN film. Scanning electron microscopy results show that the surface roughness of the sample increases when the sample is relaxed. High-resolution transmission electron microscopy images of the structure of indium droplets in the DH sample indicate that the thickness of the InGaN layer decreases with the density of indium droplets. The formation of these droplets is attributed to the insufficient kinetic energy of indium atom to react with the elements of group V, resulting to aggregation. The gallium atoms in the GaN thin film will not be uniformly replaced by indium atoms; the InGaN thin film has an uneven distribution of indium atoms and the quality of the epitaxial layer is degraded.
Receptor-interacting protein kinase 3 deficiency inhibits immune cell infiltration and attenuates organ injury in sepsis
Authors: Sharma Archna , Matsuo Shingo , Yang Weng-Lang , Wang Zhimin , Wang Ping 
Published: July 2014

Abstract
Introduction
Sepsis is defined as a systemic hyper-inflammatory immune response, with a subsequent immune-suppressive phase, which leads to multiple organ dysfunction and late lethality. Receptor-interacting protein kinase 3 (RIPK3)-dependent necrosis is implicated in driving tumor necrosis factor alpha (TNF-α)- and sepsis-induced mortality in mice. However, it is unknown if RIPK3 deficiency has any impact on immune cell trafficking, which contributes to organ damage in sepsis.

Methods
To study this, male wild-type (WT) and RIPK3-deficient (Ripk3-/-) mice on C57BL/6 background were subjected to sham operation or cecal ligation and puncture (CLP)-induced sepsis. Blood and tissue samples were collected 20 hours post-CLP for various measurements.

Results
In our severe sepsis model, the mean survival time of Ripk3-/- mice was significantly extended to 68 hours compared to 41 hours for WT mice. Ripk3-/- mice had significantly decreased plasma levels of TNF-α and IL-6 and organ injury markers compared to WT mice post-CLP. In the lungs, Ripk3-/- mice preserved better integrity of microscopic structure with reduced apoptosis, and decreased levels of IL-6, macrophage inflammatory protein (MIP)-2 and keratinocyte-derived chemokine (KC), compared to WT. In the liver, the levels of MIP-1, MIP-2 and KC were also decreased in septic Ripk3-/- mice. Particularly, the total number of neutrophils in the lungs and liver of Ripk3-/- mice decreased by 59.9% and 66.7%, respectively, compared to WT mice post-CLP. In addition, the number of natural killer (NK) and CD8T cells in the liver decreased by 64.8% and 53.4%, respectively, in Ripk3-/- mice compared to WT mice post-sepsis.

Conclusions
Our data suggest that RIPK3 deficiency modestly protected from CLP-induced severe sepsis and altered the immune cell trafficking in an organ-specific manner attenuating organ injury. Thus, RIPK3 acts as a detrimental factor in contributing to the organ deterioration in sepsis.
Nanoparticle labeling identifies slow cycling human endometrial stromal cells
Authors: Xiang Lina , Chan Rachel W S , Ng Ernest H Y , Yeung William S B 
Published: July 2014

Abstract
Introduction
Evidence suggests that the human endometrium contains stem or progenitor cells that are responsible for its remarkable regenerative capability. A common property of somatic stem cells is their quiescent state. It remains unclear whether slow-cycling cells exist in the human endometrium. We hypothesized that the human endometrium contains a subset of slow-cycling cells with somatic stem cell properties. Here, we established an in vitro stem cell assay to isolate human endometrial-derived mesenchymal stem-like cells (eMSC).

Methods
Single-cell stromal cultures were initially labeled with fluorescent nanoparticles and a small population of fluorescent persistent cells (FPC) remained after culture of 21 days. Two populations of stromal cells, namely FPC and non-FPC were sorted.

Results
Quantitative analysis of functional assays demonstrated that the FPC had higher colony forming ability, underwent more rounds of self-renewal and had greater enrichment of phenotypically defined prospective eMSC markers: CD146+/CD140b+ and W5C5+ than the non-FPC. They also differentiate into multiple mesenchymal lineages and the expression of lineage specific markers was lower than that of non-FPC. The FPC exhibit low proliferation activities. A proliferation dynamics study revealed that more FPC had a prolonged G1 phase.

Conclusions
With this study we present an efficient method to label and isolate slow-proliferating cells obtained from human endometrial stromal cultures without genetic modifications. The FPC population could be easily maintained in vitro and are of interest for tissue-repair and engineering perspectives. In summary, nanoparticle labeling is a promising tool for the identification of putative somatic stem or progenitor cells when their surface markers are undefined.
Integrins and epithelial cell polarity
Authors: Lee Jessica L. , Streuli Charles H. 
Published: July 2014

Cell polarity is characterised by differences in structure, composition and function between at least two poles of a cell. In epithelial cells, these spatial differences allow for the formation of defined apical and basal membranes. It has been increasingly recognised that cell–matrix interactions and integrins play an essential role in creating epithelial cell polarity, although key gaps in our knowledge remain. This Commentary will discuss the mounting evidence for the role of integrins in polarising epithelial cells. We build a model in which both inside-out signals to polarise basement membrane assembly at the basal surface, and outside-in signals to control microtubule apical–basal orientation and vesicular trafficking are required for establishing and maintaining the orientation of epithelial cell polarity. Finally, we discuss the relevance of the basal integrin polarity axis to cancer.This article is part of a Minifocus on Establishing polarity. For further reading, please see related articles: ‘ERM proteins at a glance’ by Andrea McClatchey (J. Cell Sci. 127, [098343]). ‘Establishment of epithelial polarity – GEF who's minding the GAP?’ by Siu Ngok et al. (J. Cell Sci. 127, [153197]).
Cyclic Voltammetric Deposition of Poly(pyrrole-co-2-formylpyrrole)–Multiwall Carbon Nanotube Composite for Highly Specific Capacitive Electrodes
Authors: Pattananuwat Prasit , Wang Kun , Tagaya Motohiro , Kobayashi Takaomi 
Published: July 2014

Abstract
A supercapacitor composite electrode was effectively fabricated by using cyclic voltammetric deposition of poly(pyrrole-co-2-formylpyrrole)–multiwall carbon nanotube composite, P(Py-co-FPy)–MWCNT. The charge storage of the three-dimensional nanofibril of the P(Py-co-FPy)-coated MWCNT composite electrode was excellent and reached the specific capacitive value of up to 535 F g−1.
Correlation among Charge, Dielectric, and Magnetic Properties in Electron-transfer-type Spin-crossover Systems
Authors: Matsuzawa Satoshi , Tazoe Kou , Nojiri Hiroyuki , Iijima Fumichika , Oshio Hiroki 
Published: July 2014

Abstract
Interplay among multiple degrees of freedoms in electron-transfer-type spin-crossover systems has been investigated by using a multiprobe system evaluating dielectric, optical, and transport properties. Increase in conductivity associated with a change in optical spectrum is found at around the spin-crossover temperature between the low- and high-spin states in [Co((R)-pabn)][Fe(Tp)(CN)3](BF4)·MeOH·2H2O, where (R)-pabn: (R)-N2,N(2′)-bis(pyridin-2-ylmethyl-1,1′-binaphthyl-2,2′-diamine and Tp: hydrotris(pyrazollyl)borate. In the dielectric constant, a very slow dielectric dispersion is found to grow in the high-spin state, showing that some macroscopic motion and/or fluctuation exist.
Structure and Magnetic Properties of Co, Mn, Mg, and Al Codoped Nickel Ferrites Prepared from Laterite Leaching Solutions
Authors: Gao Jian-ming , Zhang Mei , Guo Min 
Published: July 2014

Abstract
Metal codoped nickel ferrites were successfully prepared from laterite leaching solutions. X-ray diffraction (XRD) results indicate that all the samples are well-crystallized NiFe2O4 with face-centered cubic structure, which can be further confirmed by Fourier transform infrared spectrometry (FTIR). Scanning electron microscope (SEM) images show that the average diameters of the synthesized ferrite particles with uniform size is about 70–100 nm. Moreover, it is demonstrated that the samples exhibit typical and enhanced ferrimagnetic properties at room temperature.
Reductive Nucleation of Palladium Nanoparticles on a Cycloolefin Polymer Surface Oxidized with Active Oxygen Species Generated by Vacuum Ultraviolet Excitation
Authors: Sugimura Hiroyuki , Miki Naruhito , Nakamura Akihiro , Ichii Takashi 
Published: July 2014

Abstract
The ability to reduce palladium (Pd) ions to Pd nanoparticles has been provided on the surface of cycloolefin polymer (COP). The origin of this reductive function is aldehyde and alcoholic hydroxy groups formed by oxidizing the COP surface with active oxygen species, which were generated through the irradiation of atmospheric oxygen and water molecules with vacuum ultraviolet light of 172 nm in wavelength. The Pd nanoparticles successfully served as catalysts for electroless plating.
Spatial Turn im Archiv- und Bibliothekswesen
Authors: Mesenburg Peter , Neuheuser Hanns Peter 
Published: July 2014

Zusammenfassung
Die Untersuchung behandelt in ihrem ersten Teil die oft erhobene Forderung nach einem „Spatial Turn“ in den Geisteswissenschaften sowie speziell im Archiv- und Bibliothekswesen bezüglich der Erschließung von Kartenbeständen. Der neue Vorschlag führt zur Implementierung der Genauigkeitsprüfung für Karten in die Arbeitsabfolge. Zum ersten Mal wird dieses Verfahren für großmaßstäbige Manuskriptkarten vorge­schlagen. Im zweiten Teil des Beitrags wird die rechnergestützte Genauigkeitsanalyse der Karten des Klevischen Katasters (1731–1738) exemplarisch beschrieben. Unter Einsatz der Open-Source-Software MapAnalyst wird am Beispiel der im Gemeindearchiv Schermbeck lagernden Manuskriptkarte 7 ein Weg aufgezeigt, wie die Genauigkeit der graphischen Darstellung der im Jahre 1733 aufgemessenen Grundstücksgrenzen ermittelt werden kann. Im Rahmen der Untersuchung ergaben sich in vielen Bereichen überraschende Übereinstimmungen der Grundstücksstrukturen aus dem Jahr 1733 mit denen des Jahres 2013, die auch bei partieller Analyse zu konstanten Genauigkeitswerten führten. Nach ausgleichender Koordinatentransformation und robuster Schätzung wurden generell und auch in Teilbereichen für die dargestellten Grenzpunkte Standardabweichungen zwischen ±2,3 – ±2,9 m ermittelt.
Tilman Nagels Kritik an der Isnad-cum-matn-Analyse. Eine Replik
Authors: Görke Andreas , Motzki Harald 
Published: July 2014

Abstract
In a recent issue of Arabica, Tilman Nagel published a fundamental critique of the isnād-cum-matn analysis. He claims that the method is a formalistic procedure that is incapable of producing any relevant results with regard to the life of Muḥammad and that is to be regarded as a step backwards in the study of the historical Muḥammad. He also criticises the proponents of this method – most notably Gregor Schoeler and Harald Motzki – and accuses them of methodological flaws and scholarly naïveté. In particular, he argues that the proponents of this method wrongly claim that it allows the identification of ‘authentic’ traditions about the life of Muḥammad.
The following article offers a critical review of Nagel's arguments. It shows that Nagel's criticism is fraught with misunderstandings and misrepresentations of the works he discusses and that his accusations are thus unwarranted and untenable. The article also explains what the isnād-cum-matn analysis actually is and how it can be used for dating traditions, which is – in contrast to Nagel's claims – the method's primary objective.
Verse über Verse. Wie Ibn al-Ḥaǧǧāǧ seine eigene Poesie betrachtet
Authors: Wagner Ewald 
Published: July 2014

Abstract
Several Arabic poets composed verses on poetry in general, and on their own poetry in particular. Some of these metapoetic reflections already have been of interest to the Arabists, and became the subject of literary studies (e.g. Lyons, Schoeler, Fakhreddine, McKinney). If I again took up the topic with the focus on Ibn al-Ḥaǧǧāǧ (d. 1001), so it is because he referred to his own poetry much more often than other poets did. He had reason for this: Ibn al-Ḥaǧǧāǧ became famous – or one could also say infamous – because the new theme of suḫf (scatological obscenety) played an important role in his poetry. So he had to give arguments in support of his way of composing. The article presents a selection from his self-references which show that he not only defends his suḫf, but also boasts of this addition to Arabic poetry, which made his poems spread all over the countries.
Multi-component synthesis of 1,2-diphenyl-2-[2-(5-aryl-6H-1,3,4-thiadiazin-2-yl)hydrazono]ethanone
Authors: Aychiluhim Tewodros Birhanu , Vedula Rajeswar Rao 
Published: July 2014

Abstract
The reaction of thiocarbohydrazide with benzil and a substituted phenacyl bromide provides a facile synthetic route to a 1,2-diphenyl-2-[2-(5-aryl-6H-1,3,4-thiadiazin-2-yl)hydrazono]ethanone. The synthesized compounds were characterized by spectral and analytical data.
A Finite-difference Scheme Combining with Finite-volume Technique by Using General Quadrilateral Cells
Authors: Zhu Zheming , Zeng Ligang , Liu Kai , Fu Yangcheng 
Published: July 2014

Abstract
A finite-difference scheme by using general quadrilateral cells was developed, and a method of quarter integration path was proposed and was applied in the calculation of node forces. By using the technique of finite-volume and Green's integration formula, the finite-difference equations were obtained, and the equations were programmed by using VB.NET program language, and a code DMFD was developed. By using this code, a P-wave propagating along a finite-length rock bar was simulated, and the numerical results agree very well with the theoretical results as well as the results from ABAQUS code. Finally, the in-situ blasting in a hydropower station was simulated by using DMFD code, and the results generally agree with the in-situ results as well as those predicted by UDEC code.
Association of urinary RBP4 with insulin resistance, inflammation, and microalbuminuria
Authors: Park Se Eun , Lee Nam Seok , Park Ji Woo , Rhee Eun-Jung , Lee Won-Young , Oh Ki-Won , Park Sung-Woo , Park Cheol-Young , Youn Byung-Soo 
Published: July 2014

ObjectiveSerum concentrations of retinol-binding protein 4 (RBP4) are elevated in type 2 diabetes and associated with the severity of insulin resistance; however, there are few data about the relationship between urinary RBP4 levels and metabolic parameters. We assessed urinary RBP4 as a new biomarker by establishing its relationship with clinical parameters associated with insulin resistance and urinary albumin excretion.Design and methodsWe measured RBP4 in the serum and urine of 689 subjects with diverse glucose tolerance status. We also evaluated the relationship between urinary RBP4 and cardiometabolic risk factors, including insulin resistance, high-sensitivity C-reactive protein (hsCRP), arterial stiffness, and microalbuminuria.ResultsUrinary RBP4 levels were higher in insulin-resistant subjects with prediabetes or type 2 diabetes than in subjects with normal glucose tolerance (NGT) (type 2 diabetes>prediabetes>NGT; allP<0.001). Urinary RBP4 correlated strongly with homeostasis model assessments of insulin resistance (HOMA-IR), fasting glucose, triglycerides, blood pressure, hsCRP, arterial stiffness, estimated glomerular filtration rate, and urinary albumin-to-creatinine ratio (allP<0.01). HOMA-IR and arterial stiffness were found to be independent determinants of urinary RBP4 concentration. Furthermore, urinary RBP4 was highly predictive of microalbuminuria (odds ratio 2.6, 95% CI 1.6–4.2), even after adjustment for other metabolic parameters. The area under the ROC curve for urinary RBP4 to detect the presence of microalbuminuria was 0.80±0.02 (95% CI 0.76–0.84) and the cut-off value was 157.01 μg/gCr.ConclusionsUrinary RBP4 concentrations were elevated in patients with dysregulation of glucose metabolism and were related to various cardiometabolic risk factors including insulin resistance, inflammation, and microalbuminuria.