bluuebunny/crossref_metadata_2025_split huggingface dataset:
Global DNA methylation status in laryngeal cancer
Authors: Stembalska Agnieszka , Leszczyński Przemysław , Gil Justyna , Ramsey David , Pitala Grzegorz , Maciejczyk Adam , Frączek Marcin 
Published: June 2013

BackgroundThe purpose of our study was to evaluate if DNA methylation level in leukocytes may be used as a surrogate marker of genome methylation status in laryngeal cancer tissues.MethodsWe evaluated global DNA methylation using an ultra performance liquid chromatography (UPLC)‐based method to assess the total content of 5‐methylcytosine (5 mC).ResultsThe study was performed on DNA isolated from cancer tissues, adjacent normal tissues, and peripheral blood leukocytes in a group of 72 patients with laryngeal cancer. DNA hypomethylation was found in tumor tissue (56%) and normal tissue (49%). There was a significant correlation between the levels of 5 mC in these 2 types of tissue. There was no significant DNA hypomethylation in blood. A negative correlation between tumor grade and blood levels of 5 mC was found.ConclusionThe level of leukocyte DNA methylation measured using total 5 mC content cannot be used as a surrogate marker for genome methylation status in laryngeal cancer tissues. © 2013 Wiley Periodicals, Inc. Head Neck 36: 419–424, 2014
Prevalence and trends of human papillomavirus in oropharyngeal cancer in a predominantly north Indian population
Authors: Bahl Ankur , Kumar Pankaj , Dar Lalit , Mohanti Bidhu K. , Sharma Atul , Thakar Alok , Karthikeyan Venkat , Sikka Kapil , Singh Chiromamit , Poo Kamala , Lodha Jaini 
Published: June 2013

BackgroundHuman papillomavirus (HPV) prevalence in oropharyngeal squamous cell cancer (SCC) remains variable and studies have estimated that up to 60% may be positive.MethodsOne hundred five treatment‐naïve oropharyngeal SCC patients were included. HPV genotyping was done by consensus polymerase chain reaction (PCR) and reverse line‐blot hybridization assay. HPV prevalence was co‐related with sex, age, tobacco consumption, alcohol use, and high‐risk sexual behavior.ResultsHPV prevalence was 22.8%. No significant associations were seen between tobacco or alcohol consumption with HPV status. The mean number of lifetime sexual partners and indulgence in high‐risk sexual behavior was significantly more in patients who are HPV positive. There were no significant associations between the 2 groups with respect to the stage of the tumor.ConclusionResults of this study confirms that patients who are HPV positive are younger, and with high‐risk sexual behavior. We did not find any impact of smoking and alcohol consumption on HPV status. © 2013 Wiley Periodicals, Inc. Head Neck 36: 505–510, 2014
Feasibility of super‐selective neck dissection for indeterminate lateral neck nodes in papillary thyroid carcinoma
Authors: Kim Heejin , Jin Young Ju , Cha Wonjae , Jeong Woo‐Jin , Ahn Soon‐Hyun 
Published: June 2013

BackgroundTherapeutic lateral compartment neck dissection is recommended for cases of papillary thyroid carcinoma with biopsy‐proven lymph node metastasis. The purpose of this study was to evaluate the efficacy and safety of super‐selective neck dissection for patients with clinically suspicious lymph node metastasis not confirmed by biopsy.MethodsAmong 620 patients treated for papillary thyroid carcinoma between 2008 and 2010, 34 had suspicious lymph node enlargement in the lateral neck and underwent total thyroidectomy with super‐selective neck dissection.ResultsMetastatic disease was confirmed in 38.2% patients (13 of 34) with indeterminate lymph nodes identified by preoperative CT and/or ultrasonography who underwent super‐selective neck dissection. Most harvested lymph nodes were located at level IV. There was no recurrence during a mean follow‐up period of 31.6 months.ConclusionPatients with clinically suspicious lateral neck nodes that are not confirmed by biopsy may be good candidates for super‐selective neck dissection, which had minimal morbidity and did not compromise oncologic outcomes. © 2013 Wiley Periodicals, Inc. Head Neck 36: 487–491, 2014
Use of fallout tracers 7Be, 210Pb and 137Cs to distinguish the form of sub‐surface soil erosion delivering sediment to rivers in large catchments
Authors: Hancock Gary J. , Wilkinson Scott N. , Hawdon Aaron A. , Keen Rex J. 
Published: June 2013

AbstractFallout radionuclides (FRNs) 137Cs and 210Pb are well established as tracers of surface and sub‐surface soil erosion contributing sediment to river systems. However, without additional information, it has not been possible to distinguish sub‐surface soil erosion sources. Here, we use the FRN 7Be (half‐life 53 days) in combination with 137Cs and excess 210Pb to trace the form of erosion contributing sediment in three large river catchments in eastern Australia; the Logan River (area 3700 km2), Bowen River (9400 km2) and Mitchell River (4700 km2). We show that the combination of 137Cs, excess 210Pb and 7Be can discriminate horizontally aligned sub‐surface erosion sources (rilled and scalded hillslopes and the floors of incised drainage lines and gully ‘badland’ areas) from vertical erosion sources (channel banks and gully walls). Specifically, sub‐surface sources of sediment eroded during high rainfall and high river flow events have been distinguished by the ability of rainfall‐derived 7Be to label horizontal soil surfaces, but not vertical. Our results indicate that in the two northern catchments, erosion of horizontal sub‐surface soil sources contributed almost as much fine river sediment as vertical channel banks, and several times the contribution of hillslope topsoils. This result improves on source discrimination provided previously and indicates that in some areas erosion of hillslope soils may contribute significantly to sediment yield, but not as topsoil loss. We find that in north‐eastern Australia, scalded areas on hillslopes and incising drainage lines may be sediment sources of comparable importance to vertical channel banks. Previous studies have used the combination of 137Cs, excess 210Pb and 7Be to estimate soils losses at the hillslope scale. Here, we show that with timely and judicious sampling of soil and sediment during and immediately after high flow events 7Be measurements can augment fallout 137Cs and 210Pb to provide important erosion source information over large catchments. Copyright © 2013 John Wiley & Sons, Ltd.
Periostin Mediates the Increased Pro‐Angiogenic Activity of Gastric Cancer Cells under Hypoxic Conditions
Authors: Qiu Feng , Shi Chun‐hua , Zheng Jun , Liu Yu‐bin 
Published: June 2013

ABSTRACTThis study was conducted to investigate the biological role of periostin in gastric cancer (GC) under hypoxia. Western blot analysis revealed that along with an upregulation of hypoxia‐inducible factor‐1alpha, there was a time‐dependent induction of periostin in MKN‐45 cells under hypoxia (2% O2), increasing by eightfold as compared to normoxic cells. Pretreatment with 30 µM PD98059, an inhibitor of ERK1/2, significantly reduced hypoxia‐stimulated periostin expression (P < 0.01). Periostin knockdown in MKN‐45 cells was achieved by specific small interfering RNA (siRNA). The conditioned medium from periostin siRNA‐transfected MKN‐45 cells induced significantly less (P < 0.01) endothelial tube formation than control siRNA‐transfected cells. Additionally, periostin silencing markedly decreased the mRNA expression and secretion of vascular endothelial growth factor (VEGF) in hypoxic MKN‐45 cells. Thus, our data suggest that periostin is a hypoxia‐response gene and mediates a cross talk between GC and endothelial cells under hypoxia, partially through regulation of the VEGF expression. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:364‐369, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21498
Spatial and seasonal variability of dissolved organic matter in the Cariaco Basin
Authors: Lorenzoni Laura , Taylor Gordon T. , Benitez‐Nelson Claudia , Hansell Dennis A. , Montes Enrique , Masserini Robert , Fanning Kent , Varela Ramón , Astor Yrene , Guzmán Laurencia , Muller‐Karger Frank E. 
Published: June 2013

AbstractDissolved organic carbon (DOC), nitrogen (DON), and phosphorus (DOP) were measured monthly at the CARIACO Time Series station (10°30′N, 64°40′W) in the southeastern Caribbean Sea between 2005 and 2012. Marked seasonal variability in DOC concentrations was observed, with lower values (~66 µM) in the upper water column (<75 m) during the upwelling season (December–April) due to the injection of cool, DOC‐impoverished Subtropical Underwater from the Caribbean Sea. During the rainy season (May–November) waters were stratified and upper layer DOC concentrations increased to ~71 µM. Interannual variability in surface (1 m) concentrations of DOC was also observed in response to the variable strength in upwelling and stratification that the Cariaco Basin experienced. DON and DOP showed no such seasonality. At depths >350 m, DOC concentrations were 56 ± 4.7 µM, roughly 10 µM higher than those in the Caribbean Sea over the same depth range. DON and DOP showed similar vertical profiles to that of DOC, with higher concentrations (6.8 ± 1.2 µM N and 0.15 ±0.09 µM P) in the upper water column and invariant, lower concentrations at depth (4.8 ± 1.6 µM N and 0.10 ± 0.08 µM P). Wind‐driven advection of surface DOC out of the Cariaco Basin was estimated to support a net export ~15 Gmol C yr−1 into the Caribbean Sea; this rate is comparable to the flux of settling particulate organic carbon to depths >275 m within the basin.
Tongue suspension
Authors: Handler Ethan , Hamans Evert , Goldberg Andrew N. , Mickelson Samuel 
Published: June 2013

Objectives/HypothesisOur first objective was to perform a systematic review of suture‐based tongue suspension procedures as a stand‐alone therapy for hypopharyngeal obstruction in obstructive sleep apnea (OSA). A second objective compared outcomes of tongue suspension as part of a multilevel approach to OSA surgery to genioglossus advancement (GA) with uvulopalatopharyngoplasty (UPPP), and to genioglossus advancement with hyoid suspension (GAHM) with UPPP.Study DesignSystematic review.MethodsThe PubMed database was queried for English‐language studies published after 1997 to create four cohorts: tongue suspension alone, tongue suspension with UPPP, GA + UPPP, and GAHM with UPPP. Chi‐squared test was used to compare outcomes between cohorts.ResultsTwenty‐seven studies were included, broken down into cohorts based on our selection criteria. Six studies qualified for the tongue suspension‐alone group with a surgical success rate of 36.6%. Eight studies qualified for our cohort of tongue suspension with UPPP with a surgical success rate of 62.3%. Eighteen studies qualified for our remaining two cohorts: GA + UPPP, and GAHM + UPPP. Their surgical success rates were both 61.1%. A chi‐squared test to compare surgical outcomes showed that there was no difference between tongue suspension with UPPP, GA + UPPP, and GAHM + UPPP.ConclusionTongue suspension is effective and safe as part of a multilevel surgical approach for patients with OSA. As a stand‐alone procedure, its success rate is 36.6%, comparable to UPPP procedures for OSA success rates across the board. Tongue suspension should be considered in patients with OSA who demonstrate tongue base obstruction.Level of Evidence4. Laryngoscope, 124:329–336, 2014
Children and adults with thrombotic thrombocytopenic purpura associated with severe, acquired Adamts13 deficiency: Comparison of incidence, demographic and clinical features
Authors: Reese Jessica A. , Muthurajah Darrshini S. , Hovinga Johanna A. Kremer , Vesely Sara K. , Terrell Deirdra R. , George James N. 
Published: June 2013

AbstractBackgroundThrombotic thrombocytopenic purpura (TTP) associated with severe, acquired ADAMTS13 deficiency is uncommonly reported in children. The incidence, demographic, and clinical features of these children, compared to adults, have not been described.ProceduresThis study focused on children (<18 years old) and adults with TTP associated with severe, acquired ADAMTS13 deficiency, defined as activity <10%. The incidence rates for TTP in children and adults were calculated from patients enrolled in the Oklahoma TTP–HUS (Hemolytic–Uremic syndrome) Registry, 1996–2012. To describe demographic and clinical features, children with TTP were also identified from a systematic review of published reports and from samples sent to a reference laboratory for analysis of ADAMTS13.ResultsThe standardized annual incidence rate of TTP in children was 0.09 × 106 children per year, 3% of the incidence rate among adults (2.88 × 106 adults per year). Among the 79 children who were identified (one from the Oklahoma Registry, 55 from published reports, 23 from the reference laboratory), TTP appeared to be more common among females, similar to the relative increased frequency of women among adults with TTP, and more common in older children. Clinical data were available on 52 children; the frequency of severe renal failure, relapse, treatment with rituximab, and systemic lupus erythematosus in these children was similar to adults with TTP.ConclusionsTTP associated with severe, acquired ADAMTS13 deficiency is uncommon in children. The demographic and clinical features of these children are similar to the features of adults with TTP. Pediatr Blood Cancer 2013;60:1676–1682. © 2013 Wiley Periodicals, Inc.
The impact of passband characteristics on imaging microwave radiometer brightness temperatures over the ocean
Authors: Bettenhausen Michael H. , Adams Ian S. 
Published: June 2013

AbstractRadiative transfer modeling is used to estimate the effects of nonideal receiver frequency passband characteristics on the measured brightness temperatures from imaging microwave radiometers over the ocean. The analysis includes microwave frequencies from 6 to 40 GHz and applies to the lower frequency channels of conically scanning, space‐based radiometers such as AMSR‐E, SSMI, SSMIS, and WindSat. The analysis demonstrates that frequency passband characteristics can have significant effects on the brightness temperatures for microwave imaging channels. The largest effects are due to shifts in the center frequency of the passband. The imaging channels near the water vapor resonance at 22.235 GHz are most sensitive to passband characteristics. The effects for these channels depend on the water vapor in the scene.
The use of reflected GPS signals to retrieve ocean surface wind speeds in tropical cyclones
Authors: Katzberg Stephen J. , Dunion Jason , Ganoe George G. 
Published: June 2013

AbstractSince the first intentional acquisition of GPS signals reflected from water bodies, one of the objectives which has driven the research is to determine whether the acquired signal can provide useful geophysical information about the reflecting surface. One obvious condition of considerable interest is ocean surface wind speed. Theory suggested that the reflection technique, a form of bistatic RADAR, would be sensitive to surface roughness which in turn is driven by wind speed. This paper reports the results derived from data acquired over the past decade of applying the GPS reflection technique to ocean surface winds, particularly ocean surface winds in tropical cyclones. Examples of wind speed retrievals will be given for some illustrative cases of hurricanes and tropical storms. The results from several hurricanes and tropical storms on how the signal was calibrated will be presented. In addition, a quantitative comparison will be given between dropsondes deployed by NOAA during the storms and GPS reflection derived wind speeds taken at the same time. Conditions in which the GPS technique offers excellent comparisons as well as examples where the comparison is not so good will be presented. Suggestions will be given as to when the GPS technique can be used with confidence and when it is likely to be at variance with other methods.
Do singing‐ground surveys reflect american woodcock abundance in the western Great Lakes region?
Authors: Nelson Matthew R. , Andersen David E. 
Published: June 2013

AbstractThe Singing‐ground Survey (SGS) is the primary monitoring tool used to assess population status and trends of American woodcock (Scolopax minor). Like most broad‐scale surveys, the SGS cannot be directly validated because there are no independent estimates of abundance of displaying male American woodcock at an appropriate spatial scale. Furthermore, because locations of individual SGS routes have generally remained stationary since the SGS was standardized in 1968, it is not known whether routes adequately represent the landscapes they were intended to represent. To indirectly validate the SGS, we evaluated whether 1) counts of displaying male American woodcock on SGS routes related to land‐cover types known to be related to American woodcock abundance, 2) changes in counts of displaying male American woodcock through time were related to changes in land cover along SGS routes, and 3) land‐cover type composition along SGS routes was similar to land‐cover type composition of the surrounding landscape. In Wisconsin and Minnesota, USA, counts along SGS routes reflected known American woodcock‐habitat relations. Increases in the number of woodcock heard along SGS routes over a 13‐year period in Wisconsin were related to increasing amounts of early successional forest, decreasing amounts of mature forest, and increasing dispersion and interspersion of cover types. Finally, the cover types most strongly associated with American woodcock abundance were represented along SGS routes in proportion to their composition of the broader landscape. Taken together, these results suggest that in the western Great Lakes region, the SGS likely provides a reliable tool for monitoring relative abundance and population trends of breeding, male American woodcock. © 2013 The Wildlife Society.
Development of a whole-cell biocatalyst with NADPH regeneration system for biosulfoxidation
Authors: Zhai Xiao-Hong , Ma Yuan-Hui , Lai Dun-Yue , Zhou Shuo , Chen Zhen-Ming 
Published: June 2013

Abstract
A formate dehydrogenase gene (fdh) originated from Candida boidinii was co-expressed in E. coli BL21 (DE3) with the cyclohexanone monooxygenase gene (chmo) cloned from Acinetobacter calcoaceticus NCIMB 9871. The co-expression system was then used as a whole-cell biocatalyst to synthesize chiral phenyl methyl sulfoxide (PMSO) from thioanisole (PMS) and the reaction conditions were investigated. When the initial concentration of PMS was 20 mM, the specific productivity of PMSO in this system was 2.07 μmol g−1 cw min−1 (cw: wet cell weight) and the ee value for the R-sulfoxide was 99 %. In contrast, when chmo was the only gene expressed in E. coli, the specific productivity of PMSO was 0.053 μmol g−1 cw min−1 with no exact enantioselectivity. Further determination of NADPH concentration in the whole-cell catalysts suggested that co-expression of fdh with chmo significantly improved NADPH supply. Thus, this whole-cell biocatalyst system is highly advantageous for the synthesis of optically pure R-sulfoxide.
Views of Genetics Health Professionals on the Return of Genomic Results
Authors: Grove Megan E. , Wolpert Maya N. , Cho Mildred K. , Lee Sandra Soo‐Jin , Ormond Kelly E. 
Published: June 2013

AbstractAs exome and whole genome sequencing become clinically available, the potential to receive a large number of clinically relevant but incidental results is a significant challenge in the provision of genomic counseling. We conducted three focus groups of a total of 35 individuals who were members of ASHG and/or NSGC, assessing views towards the return of genomic results. Participants stressed that patient autonomy was primary. There was consensus that a mechanism to return results to the healthcare provider, rather than patient, and to streamline integration into the electronic health record would ensure these results had the maximal impact on patient management. All three focus groups agreed that pharmacogenomic results were reasonable to return and that they were not felt to be stigmatizing. With regard to the return of medically relevant results, there was much debate. Participants had difficulty in consistently assigning specific diseases to ‘bins’ that were considered obligatory versus optional for disclosure. Consensus was reached regarding the importance of informed consent and pretest counseling visits to clarify what the return of results process would entail. Evidence based professional guidelines should continue to be developed and regularly revised to assist in consistently and appropriately providing genomic results to patients.
Dipalmitoyl‐Phosphatidylcholine Biosynthesis is Induced by Non‐Injurious Mechanical Stretch in a Model of Alveolar Type II Cells
Authors: Pantazi Despoina , Kitsiouli Eirini , Karkabounas Athanasios , Trangas Theoni , Nakos George , Lekka Marilena E. 
Published: June 2013

AbstractDipalmitoylphosphatidylcholine, (DP‐PtdCho), the major phospholipid component of lung surfactant is biosynthesized via a de novo pathway, the last step of which is catalyzed by CDP‐choline:cholinephosphotransferase (CPT) and two remodeling steps: a deacylation and a reacylation one, catalyzed by an acidic, Ca2+‐independent phospholipase A2 (aiPLA2) and a lyso‐phosphatidylcholine acyltransferase (LPCAT), respectively. The aim of our study was to investigate whether a low magnitude, non‐injurious static mode of mechanical stretch can induce phosphatidylcholine (PtdCho) biosynthesis and its remodeling to DP‐PtdCho in the A549 cell‐line, a model of alveolar type II cells. The deformation of A549 cells did not cause any release of lactate dehydrogenase, or phospholipids into the cell culture supernatants. An increase in PtdCho levels was observed after 1 h of static stretching, especially among the DP‐PtdCho molecular species, as indicated by targeted lipidomics approach and site‐directed fatty acyl‐chain analysis. Moreover, although sphingomyelin (CerPCho) levels were unaffected, the DP‐PtdCho/CerPCho ratio increased. Induction was observed in CPT, LPCAT and aiPLA2 enzymatic activities and gene expression. Finally, incubation of the cells with MJ33 suppressed aiPLA2 activity and DP‐PtdCho production. Our data suggest that mild static mechanical stretch can promote the biosynthesis of PtdCho and its remodeling to DP‐PtdCho in lung epithelial cells. Thus, low magnitude stretch could contribute to protective mechanisms rather than to injurious ones.
Combined Orbital Floor and Medial Wall Fractures Involving the Inferomedial Strut: Repair Technique and Case Series Using Preshaped Porous Polyethylene/Titanium Implants
Authors: Cho Raymond I. , Davies Brett W. 
Published: June 2013

Background Combined orbital floor and medial wall fractures can be technically challenging to repair, particularly when the inferomedial strut is involved. A surgical repair technique is described utilizing a single preshaped porous polyethylene/titanium implant to span both defects. Methods Retrospective interventional case series. Results Fracture repair was performed on 17 orbits (16 patients) between October 2009 and February 2012. Subsequent surgical revision was required in three cases (18%). Visual acuity was stable or improved in all cases. Of 7 patients with preoperative diplopia, 5 improved and 2 remained stable postoperatively, and there were no cases of new or worsened diplopia following surgery. Postoperative asymmetry in Hertel exophthalmometry averaged 1.0 mm (range 0 to 2 mm). Preoperatively, average orbital volume was 122.7% compared with control (range 109 to 147%, standard deviation [SD] 9.6), which improved to 100.3% postoperatively (range 92 to 110%, SD 5.7). The average decrease in orbital volume was 22.5% (range 10 to 54%, SD 11.4, p < 0.001). Conclusions With careful preoperative planning and meticulous surgical technique, combined orbital floor and medial wall fractures involving the inferomedial strut can be successfully repaired with a preshaped porous polyethylene/titanium implant through a transconjunctival/transcaruncular approach with inferior oblique disinsertion.