bluuebunny/crossref_metadata_2025_split huggingface dataset:
ErbB4 Activated p38γ MAPK Isoform Mediates Early Cardiogenesis Through NKx2.5 in Human Pluripotent Stem Cells
Authors: Ramachandra Chrishan J.A. , Mehta Ashish , Wong Philip , Shim Winston 
Published: September 2015

Abstract
Activation of ErbB4 receptor signaling is instrumental in heart development, lack of which results in embryonic lethality. However, mechanism governing its intracellular signaling remains elusive. Using human pluripotent stem cells, we show that ErbB4 is critical for cardiogenesis whereby its genetic knockdown results in loss of cardiomyocytes. Phospho-proteome profiling and Western blot studies attribute this loss to inactivation of p38γ MAPK isoform which physically interacts with NKx2.5 and GATA4 transcription factors. Post-cardiomyocyte formation p38γ/NKx2.5 downregulation is followed by p38α/MEF2c upregulation suggesting stage-specific developmental roles of p38 MAPK isoforms. Knockdown of p38γ MAPK similarly disrupts cardiomyocyte formation in spite of the presence of NKx2.5. Cell fractionation and NKx2.5 phosphorylation studies suggest inhibition of ErbB4-p38γ signaling hinders NKx2.5 nuclear translocation during early cardiogenesis. This study reveals a novel pathway that directly links ErbB4 and p38γ to the transcriptional machinery of NKx2.5-GATA4 complex which is critical for cardiomyocyte formation during mammalian heart development.
The Quality of Genetic Counseling and Connected Factors as Evaluated by Male BRCA1/2 Mutation Carriers in Finland
Authors: Kajula Outi , Kääriäinen Maria , Moilanen Jukka S. , Kyngäs Helvi 
Published: September 2015

AbstractThere is little written about the quality of genetic counseling for men with the BRCA1/2 mutation. The purpose of this study was to describe the quality of genetic counseling and connected factors according to Finnish male BRCA1/2 mutation carriers’ (n = 35) perspectives and reasons for seeking genetic counseling. Data were collected from the Departments of Clinical Genetics at five Finnish university hospitals. The exploratory study design was conducted using a 51‐item questionnaire based on a previously devised quality of counseling model and analyzed using non‐parametric tests and principle content analysis. The satisfaction level with genetic counseling was high, especially with regard to the content of genetic counseling. The benefit of genetic counseling on the quality of life differed significantly (p < 0.001–0.009) from other factors. In particular, genetic counseling was in some cases associated to reduce the quality of life. Only 49 % of the male carriers felt they received sufficient counseling on social support. Attention to individual psychosocial support was proposed as an improvement to genetic counseling. Primary and secondary reasons for seeking genetic counseling and background information, such as education, affected the perceived quality of genetic counseling. The results of the study could be used to tailor genetic counseling for male BRCA1/2 mutation carriers.
Identification of Recycled Cooking Oil and Edible Oils by Iodine Determination and Carbon Isotopic Analysis
Authors: Lu Hai , Wei Chao , Fu Hui , Li Xiuqin , Zhang Qinghe , Wang Jun 
Published: September 2015

AbstractElemental iodine determination by ICP‐MS and carbon isotope analysis by EA‐IRMS were jointly employed to identify recycled cooking oil from edible oils. Iodine in 204 oils demonstrated that recycled cooking oils can be distinguished from soybean oil, maize oil, colza oil, peanut oil, sunflower oil and olive oil with the exception of tea oil and mixed oil. These two types of oils can be definitively distinguished from recycled cooking oil in the carbon isotope ratio analysis. It was proposed to use total iodine analysis as the primary screening method and the carbon isotope ratio measurement as a secondary method for confirmation and verification. For routine screening, threshold values of Iodine determination and δ13C measurement were determined to be 20 ng g−1 iodine and a range of −27.3 to −28.5 of δ13C, respectively.
Supraclavicular skin temperature and BAT activity in lean healthy adults
Authors: van der Lans Anouk A. J. J. , Vosselman Maarten J. , Hanssen Mark J. W. , Brans Boudewijn , van Marken Lichtenbelt Wouter D. 
Published: September 2015

Abstract
The ‘gold standard’ for measuring brown adipose tissue (BAT) in humans is [18F]FDG-PET/CT-imaging. With this technique subjects are exposed to ionizing radiation and are therefore limited in the number of scans that can be performed. We investigated the relation between supraclavicular skin temperatures and BAT activity values using a strictly temperature-controlled air-cooling protocol. Data of 36 male subjects was analyzed. BAT activity was evaluated by [18F]FDG-PET/CT-imaging and skin temperature was measured by means of wireless temperature sensors. Supraclavicular skin temperature dropped less compared to skin temperatures at other sites (all P values <0.01). A significant positive correlation was found between the change in supraclavicular skin temperature with BAT activity (R
2 0.23), and the change in supraclavicular skin temperature and non-shivering thermogenesis (R
2 0.18, both P values <0.01). The correlations indicate that supraclavicular skin temperature (changes) can potentially be used as a qualitative measure of BAT activity and BAT thermogenesis.
Frontier studies on fatigue, autonomic nerve dysfunction, and sleep-rhythm disorder
Authors: Tanaka Masaaki , Tajima Seiki , Mizuno Kei , Ishii Akira , Konishi Yukuo , Miike Teruhisa , Watanabe Yasuyoshi 
Published: September 2015

Abstract
Fatigue is defined as a condition or phenomenon of decreased ability and efficiency of mental and/or physical activities, caused by excessive mental or physical activities, diseases, or syndromes. It is often accompanied by a peculiar sense of discomfort, a desire to rest, and reduced motivation, referred to as fatigue sensation. Acute fatigue is a normal condition or phenomenon that disappears after a period of rest; in contrast, chronic fatigue, lasting at least 6 months, does not disappear after ordinary rest. Chronic fatigue impairs activities and contributes to various medical conditions, such as cardiovascular disease, epileptic seizures, and death. In addition, many people complain of chronic fatigue. For example, in Japan, more than one third of the general adult population complains of chronic fatigue. It would thus be of great value to clarify the mechanisms underlying chronic fatigue and to develop efficient treatment methods to overcome it. Here, we review data primarily from behavioral, electrophysiological, and neuroimaging experiments related to neural dysfunction as well as autonomic nervous system, sleep, and circadian rhythm disorders in fatigue. These data provide new perspectives on the mechanisms underlying chronic fatigue and on overcoming it.
Prenatal exposure to integerrimine N‐oxide enriched butanolic residue from Senecio brasiliensis affects behavior and striatal neurotransmitter levels of rats in adulthood
Authors: Sandini Thaísa M. , Udo Mariana S.B. , Reis‐Silva Thiago M. , Sanches Daniel , Bernardi Maria Martha , Flório Jorge Camilo , Spinosa Helenice de S. 
Published: September 2015

AbstractPyrrolizidine alkaloids (PAs) are toxins that are exclusively biosynthesized by plants and are commonly present in foods and herbs. PAs are usually associated with poisoning events in livestock and human beings. The aim of the present study was to evaluate the behavioral and neurochemical effects of prenatal exposure to PA integerrimine N‐oxide of rats in adulthood. Pregnant Wistar rats received integerrimine N‐oxide from the butanolic residue of Senecio brasiliensis by gavage on gestational days 6–20 at doses of 3, 6 and 9 mg/kg. During adulthood of the offspring, the following behavioral tests were performed: open‐field, plus‐maze, forced swimming, catalepsy and stereotypy. Histological analyses and monoamine levels were measured. Male offspring from dams that were exposed to 9 mg/kg showed an increase in locomotion in the open‐field test, an increased frequency of entries and time spent in open arms in elevated plus‐maze test, as well as decreased swimming time. In the female offspring from dams that were exposed to 9 mg/kg, there was an increased time of climbing in forced swimming and intensity of stereotyped behavior. The histological study indicates an increase in the number of multinucleated cells in the liver (6 and 9 mg/kg). In neurotransmitter analysis, specifically in the striatum, we observed change in dopamine and serotonin levels in the middle dose. Thus, our results indicate that prenatal exposure to integerrimine N‐oxide changed behavior in adulthood and neurotransmitter levels in the striatum. Our results agree with previous studies, which showed that integerrimine N‐oxide impaired physical and neurobehavioral development in childhood that can persist until adulthood.
Thomas Hooker, Martin Luther, and the Terror at the Edge of Protestant Faith
Authors: Tipson Baird 
Published: September 2015

Toward the end of his nine-year tenure as Fellow, Thomas Hooker awoke in his sleeping quarters at Emmanuel College terrified by a sense of “the Just Wrath of Heaven.” His God, the same God in whom he had always put his trust, had turned against him and was furious at his sinfulness. Dreading divine punishment, Hooker found himself “fill'd. . . with most unusual Degrees of Horror and Anguish.” Alone in the night, Hooker was haunted by the anger of a terrifying God.
The relationships between coping strategies, social support and depression: an investigation among Turkish care-givers of patients with dementia
Authors: KIRAL KAHRAMAN , YETİM ÜNSAL , ÖZGE AYNUR , AYDIN ARZU 
Published: September 2015

ABSTRACTCaring for a patient with dementia is a stressful life event, and care-givers carry a heavy psychological burden. However, the extent to which care-givers are affected by the stressful aspects of care-giving may depend on a variety of factors. This study examined the relationships between cognitive emotion regulation strategies, social support and depression among Turkish dementia care-givers. Research questions explored whether different coping strategies and social support were related to levels of depression, as well as whether social support moderated this relationship. We used a hierarchical multiple regression analysis consisting of three blocks as the primary statistical technique to examine our expectations. In total, 141 dementia care-givers (108 women, 33 men) were recruited to the study. The mean age of the sample was 59.74 years old (standard deviation = 12.70). Hierarchical regression analysis indicated a significant negative main effect for positive refocusing strategies and significant positive main effects for catastrophising and blaming others. Social support moderated the relationships between catastrophising, rumination and symptoms of depression. Our results show that cognitive emotion regulation strategies and social support can play significant roles in alleviating care-giver depression. In the light of these results, it may be suggested that interventions focusing on the effects of positive refocusing, catastrophising and blaming others, as well as providing social support, may be helpful in alleviating depression in care-givers.
A conceptual framework for economic optimization of an animal health surveillance portfolio
Authors: GUO X. , CLAASSEN G. D. H. , OUDE LANSINK A. G. J. M. , SAATKAMP H. W. 
Published: September 2015

SUMMARYDecision making on hazard surveillance in livestock product chains is a multi-hazard, multi-stakeholder, and multi-criteria process that includes a variety of decision alternatives. The multi-hazard aspect means that the allocation of the scarce resource for surveillance should be optimized from the point of view of a surveillance portfolio (SP) rather than a single hazard. In this paper, we present a novel conceptual approach for economic optimization of a SP to address the resource allocation problem for a surveillance organization from a theoretical perspective. This approach uses multi-criteria techniques to evaluate the performances of different settings of a SP, taking cost-benefit aspects of surveillance and stakeholders’ preferences into account. The credibility of the approach has also been checked for conceptual validity, data needs and operational validity; the application potentials of the approach are also discussed.
A Quantitative Method for Microtubule Analysis in Fluorescence Images
Authors: Lan Xiaodong , Li Lingfei , Hu Jiongyu , Zhang Qiong , Dang Yongming , Huang Yuesheng 
Published: September 2015

AbstractMicrotubule analysis is of significant value for a better understanding of normal and pathological cellular processes. Although immunofluorescence microscopic techniques have proven useful in the study of microtubules, comparative results commonly rely on a descriptive and subjective visual analysis. We developed an objective and quantitative method based on image processing and analysis of fluorescently labeled microtubular patterns in cultured cells. We used a multi-parameter approach by analyzing four quantifiable characteristics to compose our quantitative feature set. Then we interpreted specific changes in the parameters and revealed the contribution of each feature set using principal component analysis. In addition, we verified that different treatment groups could be clearly discriminated using principal components of the multi-parameter model. High predictive accuracy of four commonly used multi-classification methods confirmed our method. These results demonstrated the effectiveness and efficiency of our method in the analysis of microtubules in fluorescence images. Application of the analytical methods presented here provides information concerning the organization and modification of microtubules, and could aid in the further understanding of structural and functional aspects of microtubules under normal and pathological conditions.
Political Contestation, Secularization, and Religious Supply: Why is Morality Policy so Restrictive in the Faroe Islands?
Authors: van Kersbergen Kees , Lindberg Erla Leifsdóttir 
Published: September 2015

AbstractThe Nordic countries pursue permissive policies with regard to morality issues. There is, however, one exception: the Faroe Islands. This country pursues remarkably conservative policies. Drawing on morality issue theory, secularization theory, and the supply side theory of religion, we develop a framework that explains the political dynamics around morality issues and policies in this country. We contribute to the understanding of the various ways in which religion can enter politics by showing how and why a minimal politicization of morality issues can coincide with a strong religious wording of such issues in a context where a high level of religiosity stands out. Religious vitality came about because religion became attached to the nationalist and language struggle, which encouraged religious activity. The ample supply of religion accounts for religious vitality, which, in turn, explains why religion continues to play in morality politics.
Ablation of hippocampal neurogenesis in mice impairs the response to stress during the dark cycle
Authors: Tsai Cheng-Yu , Tsai Ching-Yen , Arnold Sebastian J. , Huang Guo-Jen 
Published: September 2015

AbstractThe functional role of adult neurogenesis in the hippocampus remains the subject of intense speculation. One recent hypothesis is that adult-born neurons contribute to the endocrine and behavioural outputs of the stress response. Here we show a genetic model system to ablate neurogenesis by inducibly deleting Tbr2 gene function specifically in the hippocampus and corroborate our findings in a radiation-based model of neurogenesis deprivation. We found that mice with ablation of new neurons in the dentate gyrus exhibit reduced anxiety during the dark cycle. After restraint stress, corticosterone levels in neurogenesis-deficient mice decreased more quickly than controls and were more sensitive to suppression by dexamethasone. Furthermore, glucocorticoid receptor target genes and neuronal activity markers showed reduced expression after stress in neurogenesis-deficient mice. These findings suggest that newborn neurons in the hippocampus are involved in sensing and eliciting an appropriate response to stress.
Antibiotics in neonatal life increase murine susceptibility to experimental psoriasis
Authors: Zanvit Peter , Konkel Joanne E. , Jiao Xue , Kasagi Shimpei , Zhang Dunfang , Wu Ruiqing , Chia Cheryl , Ajami Nadim J. , Smith Daniel P. , Petrosino Joseph F. , Abbatiello Brittany , Nakatsukasa Hiroko , Chen Qianming , Belkaid Yasmine , Chen Zi-Jiang , Chen WanJun 
Published: September 2015

AbstractPsoriasis is an inflammatory skin disease affecting ∼2% of the world’s population, but the aetiology remains incompletely understood. Recently, microbiota have been shown to differentially regulate the development of autoimmune diseases, but their influence on psoriasis is incompletely understood. We show here that adult mice treated with antibiotics that target Gram-negative and Gram-positive bacteria develop ameliorated psoriasiform dermatitis induced by imiquimod, with decreased pro-inflammatory IL-17- and IL-22-producing T cells. Surprisingly, mice treated neonatally with these antibiotics develop exacerbated psoriasis induced by imiquimod or recombinant IL-23 injection when challenged as adults, with increased IL-22-producing γδ+ T cells. 16S rRNA gene compositional analysis reveals that neonatal antibiotic-treatment dysregulates gut and skin microbiota in adults, which is associated with increased susceptibility to experimental psoriasis. This link between neonatal antibiotic-mediated imbalance in microbiota and development of experimental psoriasis provides precedence for further investigation of its specific aetiology as it relates to human psoriasis.
Lower glycolysis carries a higher flux than any biochemically possible alternative
Authors: Court Steven J. , Waclaw Bartlomiej , Allen Rosalind J. 
Published: September 2015

AbstractThe universality of many pathways of core metabolism suggests a strong role for evolutionary selection, but it remains unclear whether existing pathways have been selected from a large or small set of biochemical possibilities. To address this question, we constructin silicoall possible biochemically feasible alternatives to the trunk pathway of glycolysis and gluconeogenesis, one of the most highly conserved pathways in metabolism. We show that, even though a large number of alternative pathways exist, the alternatives carry lower flux than the real pathway under typical physiological conditions. We also find that if physiological conditions were different, different pathways could outperform those found in nature. Together, our results demonstrate how thermodynamic and biophysical constraints restrict the biochemical alternatives that are open to evolution, and suggest that the existing trunk pathway of glycolysis and gluconeogenesis may represent a maximal flux solution.
Raman spectroscopy as probe of nanometre-scale strain variations in graphene
Authors: Neumann C. , Reichardt S. , Venezuela P. , Drögeler M. , Banszerus L. , Schmitz M. , Watanabe K. , Taniguchi T. , Mauri F. , Beschoten B. , Rotkin S. V. , Stampfer C. 
Published: September 2015

AbstractConfocal Raman spectroscopy has emerged as a major, versatile workhorse for the non-invasive characterization of graphene. Although it is successfully used to determine the number of layers, the quality of edges, and the effects of strain, doping and disorder, the nature of the experimentally observed broadening of the most prominent Raman 2D line has remained unclear. Here we show that the observed 2D line width contains valuable information on strain variations in graphene on length scales far below the laser spot size, that is, on the nanometre-scale. This finding is highly relevant as it has been shown recently that such nanometre-scaled strain variations limit the carrier mobility in high-quality graphene devices. Consequently, the 2D line width is a good and easily accessible quantity for classifying the crystalline quality, nanometre-scale flatness as well as local electronic properties of graphene, all important for future scientific and industrial applications.
Dose–response curve slope helps predict therapeutic potency and breadth of HIV broadly neutralizing antibodies
Authors: Webb Nicholas E. , Montefiori David C. , Lee Benhur 
Published: September 2015

AbstractA new generation of HIV broadly neutralizing antibodies (bnAbs) with remarkable potency, breadth and epitope diversity has rejuvenated interest in immunotherapeutic strategies. Potencies defined by in vitro IC50 and IC80 values (50 and 80% inhibitory concentrations) figure prominently into the selection of clinical candidates; however, much higher therapeutic levels will be required to reduce multiple logs of virus and impede escape. Here we predict bnAb potency at therapeutic levels by analysing dose–response curve slopes, and show that slope is independent of IC50/IC80 and specifically relates to bnAb epitope class. With few exceptions, CD4-binding site and V3-glycan bnAbs exhibit slopes >1, indicative of higher expected therapeutic effectiveness, whereas V2-glycan, gp41 membrane-proximal external region (MPER) and gp120–gp41 bnAbs exhibit less favourable slopes <1. Our results indicate that slope is one major predictor of both potency and breadth for bnAbs at clinically relevant concentrations, and may better coordinate the relationship between bnAb epitope structure and therapeutic expectations.
Exciton dynamics of C60-based single-photon emitters explored by Hanbury Brown–Twiss scanning tunnelling microscopy
Authors: Merino P. , Große C. , Rosławska A. , Kuhnke K. , Kern K. 
Published: September 2015

AbstractExciton creation and annihilation by charges are crucial processes for technologies relying on charge-exciton-photon conversion. Improvement of organic light sources or dye-sensitized solar cells requires methods to address exciton dynamics at the molecular scale. Near-field techniques have been instrumental for this purpose; however, characterizing exciton recombination with molecular resolution remained a challenge. Here, we study exciton dynamics by using scanning tunnelling microscopy to inject current with sub-molecular precision and Hanbury Brown–Twiss interferometry to measure photon correlations in the far-field electroluminescence. Controlled injection allows us to generate excitons in solid C60 and let them interact with charges during their lifetime. We demonstrate electrically driven single-photon emission from localized structural defects and determine exciton lifetimes in the picosecond range. Monitoring lifetime shortening and luminescence saturation for increasing carrier injection rates provides access to charge-exciton annihilation dynamics. Our approach introduces a unique way to study single quasi-particle dynamics on the ultimate molecular scale.