bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting Glycoprotein NMB With Antibody‐Drug Conjugate, Glembatumumab Vedotin, for the Treatment of Osteosarcoma
Authors: Roth Michael , Barris David M. , Piperdi Sajida , Kuo Vicky , Everts Stephanie , Geller David , Houghton Peter , Kolb E. Anders , Hawthorne Thomas , Gill Jonathan , Gorlick Richard 
Published: August 2015

BackgroundCure rates for children and young adults with osteosarcoma have remained stagnant over the past three decades. Targeting glycoprotein non‐metastatic b (GPNMB) with the antibody‐drug conjugate glembatumumab vedotin has improved outcomes for patients with melanoma and breast cancer. The potential utility of targeting GPNMB in osteosarcoma was explored.MethodsGPNMB protein expression was evaluated by immunohistochemistry in human osteosarcoma tumor samples and by enzyme‐linked immunosorbent assay (ELISA) in osteosarcoma cell lines. mRNA expression was measured by quantitative PCR in primary osteosarcoma samples and cell lines. Surface GPNMB expression was evaluated by flow cytometry and correlated with in vitro and in vivo cytotoxicity of glembatumumab vedotin.ResultsSixty seven human osteosarcoma samples were evaluated by immunohistochemistry, including 12 samples from initial biopsy, 38 samples from definitive surgery, and 17 from the time of disease recurrence. GPNMB was expressed in 92.5% (62/67) of osteosarcoma samples. All primary osteosarcoma samples expressed high levels of GPNMB mRNA. Glembatumumab induced cytotoxic effects in 74% (14/19) of osteosarcoma cell lines, and GPNMB protein levels correlated with glembatumumab in vitro cytotoxicity (r = −0.46, P = 0.04). All osteosarcoma cell lines demonstrated surface GPNMB expression.ConclusionsGPNMB is expressed in osteosarcoma and targeting GPNMB with the antibody‐drug conjugate glembatumumab vedotin demonstrates osteosarcoma cytotoxic activity. Clinical trials are indicated to assess the efficacy of targeting GPNMB in patients with osteosarcoma. Pediatr Blood Cancer 2015; 9999:1–7 © 2015 Wiley Periodicals, Inc.
Synthesis of strigolactones, a strategic account
Authors: Zwanenburg Binne , Ćavar Zeljković Sanja , Pospíšil Tomáš 
Published: August 2015

AbstractStrigolactones (SLs) constitute a new class of plant hormones that have received growing interest in recent years. They firstly became known as signalling molecules for host recognition by parasitic plants, and for symbiosis of plants with arbuscular mycorrhizal fungi. Furthermore, they are involved in numerous physiological processes in plants, such as the regulation of plant architecture and the response to abiotic factors. SLs are produced by plants in extremely low quantities, and they may be unstable during the purification process. Therefore, their total synthesis is highly relevant for confirming the structures assigned on the basis of spectroscopic and other physical data. A second important theme in SL research is the design and synthesis of SL analogues that have a simplified structure while still featuring the essential bioproperties. This review summarises the strategy and synthesis of naturally occurring SLs, and the design and synthesis of SL analogues with appreciable bioactivity. © 2015 Society of Chemical Industry
Velocity‐free attitude stabilization with inertial vector measurements
Authors: Benziane Lotfi , Benallegue Abdelaziz , Chitour Yacine , Tayebi Abdelhamid 
Published: August 2015

SummaryThis paper deals with the attitude stabilization problem of a rigid body, where neither the angular velocity nor the attitude is used in the feedback; only body‐referenced vector measurements are needed. The proposed control scheme is based on an angular velocity observer‐like system relying solely on vector measurements. The proposed controller ensures almost global asymptotic stability and provides some interesting performance properties through an appropriate tuning of the control gains. The performance and effectiveness of the proposed control scheme are illustrated via simulation results where the control gains are adjusted using a nonlinear optimization. Copyright © 2015 John Wiley & Sons, Ltd.
Provably secure unbounded multi‐authority ciphertext‐policy attribute‐based encryption
Authors: Li Qi , Ma Jianfeng , Li Rui , Xiong Jinbo , Liu Ximeng 
Published: August 2015

AbstractMulti‐authority attribute‐based encryption (ABE) is a generation of ABE where the descriptive attributes are managed by different authorities. In current multi‐authority ABE schemes, the scale of attribute universe employed in encryption is restricted by various predefined thresholds. In this paper, we propose an unbounded multi‐authority ciphertext‐policy ABE system without such restriction. Our scheme consists of multiple attribute authorities (AAs), one central authority (CA), and users labeled by the set of attributes. Each AA governs a different universe of attributes and operates separately. Moreover, there is no cooperation between the CA and AAs. To provide the private keys for a user, the AAs first issue partial attribute‐related keys according to the attributes; the CA then issues identity‐related keys and links these attribute‐keys with the user's global identifier. Both the identity‐related and the linked attribute‐related keys will be used in decryption. The proposed multi‐authority ciphertext‐policy ABE scheme can support arbitrary linear secret sharing scheme as the access policy. Performance analysis and security proof indicate that our scheme is efficient and secure. Copyright © 2015 John Wiley & Sons, Ltd.
NEDD4 Regulates PAX7 Levels Promoting Activation of the Differentiation Program in Skeletal Muscle Precursors
Authors: Bustos Francisco , de la Vega Eduardo , Cabezas Felipe , Thompson James , Cornelison D.D.W. , Olwin Bradley B. , Yates John R. , Olguín Hugo C. 
Published: August 2015

Abstract
The transcription factor Pax7 regulates skeletal muscle stem cell (satellite cells) specification and maintenance through various mechanisms, including repressing the activity of the muscle regulatory factor MyoD. Hence, Pax7-to-MyoD protein ratios can determine maintenance of the committed-undifferentiated state or activation of the differentiation program. Pax7 expression decreases sharply in differentiating myoblasts but is maintained in cells (re)acquiring quiescence, yet the mechanisms regulating Pax7 levels based on differentiation status are not well understood. Here we show that Pax7 levels are directly regulated by the ubiquitin-ligase Nedd4. Our results indicate that Nedd4 is expressed in quiescent and activated satellite cells, that Nedd4 and Pax7 physically interact during early muscle differentiation—correlating with Pax7 ubiquitination and decline—and that Nedd4 loss of function prevented this effect. Furthermore, even transient nuclear accumulation of Nedd4 induced a drop in Pax7 levels and precocious muscle differentiation. Consequently, we propose that Nedd4 functions as a novel Pax7 regulator, which activity is temporally and spatially controlled to modulate the Pax7 protein levels and therefore satellite cell fate. Stem Cells 2015;33:3138—3151
In Vitro Conditioned Bone Marrow-Derived Mesenchymal Stem Cells Promote De Novo Functional Enteric Nerve Regeneration, but Not Through Direct-Transdifferentiation
Authors: Lin Rong , Ding Zhen , Ma Huan , Shi Huiying , Gao Yuanjun , Qian Wei , Shi Weina , Sun Zhaoli , Hou Xiaohua , Li Xuhang 
Published: August 2015

AbstractInjury or neurodegenerative disorders of the enteric nervous system (ENS) cause gastrointestinal dysfunctions for which there is no effective therapy. This study, using the benzalkonium chloride-induced rat gastric denervation model, aimed to determine whether transplantation of bone marrow-derived mesenchymal stem cells (BMSC) could promote ENS neuron regeneration and if so, to elucidate the mechanism. Fluorescently labeled BMSC, isolated from either WT (BMSC labeled with bis-benzimide [BBM]) or green fluorescent protein (GFP)-transgenic rats, were preconditioned in vitro using fetal gut culture media containing glial cell-derived neurotrophic factor (GDNF), and transplanted subserosally into the denervated area of rat pylorus. In the nerve-ablated pylorus, grafted BMSC survived and migrated from the subserosa to the submucosa 28 days after transplantation, without apparent dedifferentiation. A massive number of PGP9.5/NSE/HuC/D/Tuj1-positive (but GFP- and BBM-negative) neurons were effectively regenerated in denervated pylorus grafted with preconditioned BMSC, suggesting that they were regenerated de novo, not originating from trans-differentiation of the transplanted BMSC. BMSC transplantation restored both basal pyloric contractility and electric field stimulation-induced relaxation. High levels of GDNF were induced in both in vitro-preconditioned BMSC as well as the previously denervated pylorus after transplantation of preconditioned BMSC. Thus, a BMSC-initiated GDNF-positive feedback mechanism is suggested to promote neuron regeneration and growth. In summary, we have demonstrated that allogeneically transplanted preconditioned BMSC initiate de novo regeneration of gastric neuronal cells/structures that in turn restore gastric contractility in pylorus-denervated rats. These neuronal structures did not originate from the grafted BMSC. Our data suggest that preconditioned allogeneic BMSC may have therapeutic value in treating enteric nerve disorders. Stem Cells 2015;33:3545–3557
Bcl3 Bridges LIF-STAT3 to Oct4 Signaling in the Maintenance of Naïve Pluripotency
Authors: Chen Chen-Yun , Lee Desy S. , Yan Yu-Ting , Shen Chia-Ning , Hwang Shiaw-Min , Lee Sho Tone , Hsieh Patrick C.H. 
Published: August 2015

Abstract
Leukemia inhibitory factor (LIF) regulates mouse embryonic stem cell (mESC) pluripotency through STAT3 activation, but the downstream signaling remains largely unelucidated. Using cDNA microarrays, we verified B cell leukemia/lymphoma 3 (Bcl3) as the most significantly downregulated factor following LIF withdrawal in mESCs. Bcl3 knockdown altered mESC morphology, reduced expression of pluripotency genes including Oct4, Sox2, and Nanog, and downregulated DNA binding of acetylated histone 3 and RNA polymerase II on the Oct4 promoter. Conversely, Bcl3 overexpression partially prevented cell differentiation and promoted Oct4 and Nanog promoter activities. Furthermore, coimmunoprecipitation and chromatin immunoprecipitation experiments demonstrated that Bcl3 regulation of mESC pluripotency may be through its association with Oct4 and β-catenin and its promoter binding capability. These results establish that Bcl3 positively regulates pluripotency genes and thus shed light on the mechanism of Bcl3 as a downstream molecule of LIF/STAT3 signaling in pluripotency maintenance. Stem Cells 2015;33:3468–3480
Informal science institutions and learning to teach: An examination of identity, agency, and affordances
Authors: Adams Jennifer D. , Gupta Preeti 
Published: August 2015

AbstractInformal science education institutions play an important in the public understanding of science and, because of this are well‐positioned to positively impact science teacher education. Informal science institutions (ISIs) have a range of affordances that could contribute to learner‐centered science teacher identity development. This article describes research from a clinical experience in a museum where teacher candidates engaged visitors in learning dialogs around objects on a moveable cart in an exhibit. We describe how working in informal settings and learning to use the affordances of that setting supports aspiring teachers to connect theory to practice in ways that developed Spielraum in that is student‐centered, responsive to the needs of learners, and allows for the imagination future selves and classrooms that are conducive to maintaining these identities. This research supports the critical role that ISIs could play in teacher education, especially during the clinical phase where teacher candidates are forming initial notions about their identities, about the self who teaches. © 2015 Wiley Periodicals, Inc. J Res Sci Teach 54: 121–138, 2017
From cyst to tubule: innovations in vertebrate spermatogenesis
Authors: Yoshida Shosei 
Published: August 2015

Although vertebrates share many common traits, their germline development and function exhibit significant divergence. In particular, this article focuses on their spermatogenesis. The fundamental elements that constitute vertebrate spermatogenesis and the evolutionary changes that occurred upon transition from water to land will be discussed. The life‐long continuity of spermatogenesis is supported by the function of stem cells. Series of mitotic and meiotic germ cell divisions are ‘incomplete’ due to incomplete cytokinesis, forming syncytia interconnected via intercellular bridges (ICBs). Throughout this process, germ cells are supported by appropriate microenvironments established primarily by somatic Sertoli cells. In anamniotes (fish and amphibians) spermatogenesis progresses in cysts, in which developing germ cell syncytia are individually encapsulated by Sertoli cells. Accordingly, Sertoli cells undergo turnover with germ cells that they nourish. This mode of cystic spermatogenesis is also observed in nonvertebrates as insects. In amniotes (reptiles, birds, and mammals), however, Sertoli cells do not turn over but comprise a persistent structure of seminiferous tubules. Sertoli cells nourish different stages of germ cells simultaneously in distinct regions of their surface. This function of Sertoli cells is spatiotemporally orchestrated, and the seminiferous epithelial cycle and spermatogenic wave make the seminiferous tubules a high‐throughput factory for sperm production. Furthermore, contrary to the organized differentiating cells, undifferentiated spermatogonia that comprise the stem cell compartment exhibit active motion over the basal layer of seminiferous tubules and the frequent breakdown of ICBs. Thus, amniote seminiferous tubules represent a typical facultative (or open) niche environment without a stem cell tethering anatomically defined niche.WIREs Dev Biol2016, 5:119–131. doi: 10.1002/wdev.204This article is categorized under:Gene Expression and Transcriptional Hierarchies > Cellular DifferentiationAdult Stem Cells, Tissue Renewal, and Regeneration > Tissue Stem Cells and NichesAdult Stem Cells, Tissue Renewal, and Regeneration > Environmental Control of Stem Cells
Analytical model for predicting the performance of arbitrary size and layout wind farms
Authors: Yang Xiaolei , Sotiropoulos Fotis 
Published: August 2015

AbstractA simple engineering model for predicting wind farm performance is presented, which is applicable to wind farms of arbitrary size and turbine layout. For modeling the interaction of wind farm with the atmospheric boundary layer (ABL), the wind farm is represented as added roughness elements. The wind speed behind each turbine is calculated using a kinematic model, in which the friction velocity and the wind speed outside the turbine wake, constructed based on the wind farm‐ABL interaction model, are employed to estimate the wake expansion rate in the crosswind direction and the maximum wind speed that can be recovered within the turbine wake, respectively. Validation of the model is carried out by comparing the model predictions with the measurements from wind tunnel experiments and the Horns Rev wind farm. For all validation cases, satisfactory agreement is obtained between model predictions and experimental data. Copyright © 2015 John Wiley & Sons, Ltd.
Superposition of vortex cylinders for steady and unsteady simulation of rotors of finite tip‐speed ratio
Authors: Branlard E. , Gaunaa M. 
Published: August 2015

AbstractJoukowski introduced in 1912 a helical vortex model to represent the vorticity of a rotor and its wake. For an infinite number of blades but finite tip‐speed ratio, the model consists of a vortex cylinder of longitudinal and tangential vorticity, a root vortex and a bound vortex disk. A superposition of cylinders is used in this paper to model rotors of radially varying circulation. The relations required to form a consistent system of cylinders are derived. The model contains a term which is not accounted for in the standard blade element momentum (BEM) algorithm. This term is identified as the contribution from the pressure drop due to the wake rotation. The BEM algorithm can be corrected to account for this effect. Unlike previous work on the topic, the contribution is derived for a radially varying circulation. A high‐thrust correction is also presented to extend the model. The optimal power coefficient obtained with this model for the constant circulation rotor is assessed and compared with that of existing solutions. Results from prescribed thrust distributions are compared with that of actuator disk simulations. Steady simulations are performed to compare with the BEM algorithm. The model is also applied to compute the velocity field in the entire domain and perform unsteady simulations. Results for an unsteady simulation corresponding to a pitch change of the rotor is used to compare the model with measurements and a BEM code with a dynamic inflow model. Copyright © 2015 John Wiley & Sons, Ltd.
A Randomised Assessment of Trainee Doctors’ Understanding and Interpretation of Diagnostic Test Results
Authors: Parker V. L. , Ritchie J. E. , Drake T. M. , Hookham J. , Balasubramanian S. P. 
Published: August 2015

AbstractIntroductionDoctors are unfamiliar with diagnostic accuracy parameters despite routine clinical use of diagnostic tests to estimate disease probability.MethodsTrainee doctors completed a questionnaire exploring their understanding of diagnostic accuracy parameters; ability to calculate post‐test probability of a common surgical condition (appendicitis) and their perceptions on training in this area. To determine whether the method of information provision altered interpretation, trainees were randomised to receive diagnostic test information in three ways: positive test only; positive test with specificity and sensitivity; positive test with positive likelihood ratio in layman terms.Results326 candidates were recruited across 30 training sessions. Trainees scored a median of three out of seven in questions concerning knowledge of diagnostic accuracy parameters. This was affected neither by training level (P = 0.737) nor by experience in acute general surgery (P = 0.738). 30 (11.8 %) candidates correctly estimated post‐test probability; with 86.6 % overestimating this value. Neither level of training (P = 0.180) nor experience (P = 0.242) influenced the accuracy of the estimate. Provision of the ultrasound scan results in different ways was not associated with likelihood of a correct response (P = 0.857).ConclusionThis study highlights the deficiencies in trainee doctors’ understanding and application of diagnostic tests results. Most trainees over‐estimated disease probability, increasing the risk of unnecessary intervention and treatment.
CLA‐Rich Soy Oil Shortening Production and Characterization
Authors: Mayfield Sarah , Proctor Andrew , Shinn Sara E. , Dewettinck Koen , Patel Ashok R. 
Published: August 2015

AbstractConjugated linoleic acid‐rich soy oil (CLARSO) has been shown to have numerous health benefits, including anti‐obesity and anti‐carcinogenic properties. This oil was previously used to produce CLA‐rich margarine that showed physical characteristics similar to commercially available margarine. The objective of this study was to produce CLA‐rich shortening and analyze its physical properties relative to commercially available shortenings and soy oil control shortenings. The shortenings were prepared and their rheology, thermal behavior, and solid fat content (SFC) were determined and compared to the commercial samples. The CLA‐rich shortening samples showed similar rheological properties to the commercial samples and showed a better consistency (more solid‐like behavior) compared to the soy oil control samples. In addition, the CLA‐rich shortenings also have a higher SFC (% SFC) as well as higher latent heat of crystallization and melting than the soy oil controls indicating a comparatively higher crystalline fraction. Thus, CLARSO produced firmer shortenings than did conventional soy oil by interacting with the crystallizing stearin fraction and consequently increasing the crystalline mass fraction without significantly altering the microstructure kinetics of solid fat crystallization.
Changes in Volatile Compounds of Black Cumin Oil and Hazelnut Oil by Microwave Heating Process
Authors: Kiralan Mustafa , Kiralan S. Sezer 
Published: August 2015

AbstractBlack cumin and hazelnut oils were subjected to a heating process in a microwave oven for a duration of 2, 4, 6 and 8 min at a constant frequency of 2450 MHz and a power of 0.45 kW. The ultraviolet absorption and volatile products of the oils were investigated in detail during the processes. The experimental evidences obtained show that K232 and K270 parameters reach values of 4.69 and 1.30 for black cumin oil, 3.22 and 1.75 for hazelnut oil, respectively with the increment of heating time. The headspace SPME method was used to analyze volatile compounds extracted from black cumin and hazelnut oils being exposed to the microwave heating process. The SPME–GC/MS method allowed the detection of 17 identified volatile compounds (hexanal, α‐thujene, α‐pinene, sabinene, β‐pinene, 2‐heptenal, α‐terpinene, limonene, p‐cymene, γ‐terpinene, E‐2‐octenal, nonanal, 4‐terpineol, thymoquinone, E,E‐2,4‐decadienal, α‐longipinene and isolongifolene) in black cumin oils. Of the products, hexanal, 2‐heptenal, E‐2‐octenal, nonanal and E,E‐2,4‐decadienal were determined to be the predominant volatile oxidation products. In fact, the hexanal was found as a major volatile oxidation compound and reached a local maximum point of 7.41 × 106 AU at the end of heating. On the other hand, only 8 volatile oxidation products (hexanal, heptanal, 2‐heptenal, nonanal, E‐2‐decenal, E,Z‐2,4‐decadienal, E,E‐2,4‐decadienal and E‐2‐tridecenal) were identified in hazelnut oils as a consequence of the heating process. Based on the experimental evidence observed, it is reasonable to conclude that the nonanal content dramatically increased at the end of heating and reached a value of 9.22 × 106 AU.
Cortical thickness predicts the first onset of major depression in adolescence
Authors: Foland‐Ross Lara C. , Sacchet Matthew D. , Prasad Gautam , Gilbert Brooke , Thompson Paul M. , Gotlib Ian H. 
Published: August 2015

AbstractGiven the increasing prevalence of Major Depressive Disorder and recent advances in preventative treatments for this disorder, an important challenge in pediatric neuroimaging is the early identification of individuals at risk for depression. We examined whether machine learning can be used to predict the onset of depression at the individual level. Thirty‐three never‐disordered adolescents (10–15 years old) underwent structural MRI. Participants were followed for 5 years to monitor the emergence of clinically significant depressive symptoms. We used support vector machines (SVMs) to test whether baseline cortical thickness could reliably distinguish adolescents who develop depression from adolescents who remained free of any Axis I disorder. Accuracies from subsampled cross‐validated classification were used to assess classifier performance. Baseline cortical thickness correctly predicted the future onset of depression with an overall accuracy of 70% (69% sensitivity, 70% specificity; p = 0.021). Examination of SVM feature weights indicated that the right medial orbitofrontal, right precentral, left anterior cingulate, and bilateral insular cortex contributed most strongly to this classification. These findings indicate that cortical gray matter structure can predict the subsequent onset of depression. An important direction for future research is to elucidate mechanisms by which these anomalies in gray matter structure increase risk for developing this disorder.