bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Inherited Allele Confers Prostate Cancer Progression and Drug Resistance via RFX6/HOXA10‐Orchestrated TGFβ Signaling
Authors: Zhong Mengjie , Xu Wenjie , Tian Pan , Zhang Qin , Wang Zixian , Liang Limiao , Zhang Qixiang , Yang Yuehong , Lu Ying , Wei Gong‐Hong 
Published: June 2024

AbstractGenetic and epigenetic alterations are cancer hallmark characteristics. However, the role of inherited cancer predisposition alleles in co‐opting lineage factor epigenetic reprogramming and tumor progression remains elusive. Here the FinnGen cohort phenome‐wide analysis, along with multiple genome‐wide association studies, has consistently identified the rs339331‐RFX6/6q22 locus associated with prostate cancer (PCa) risk across diverse populations. It is uncovered that rs339331 resides in a reprogrammed androgen receptor (AR) binding site in PCa tumors, with the T risk allele enhancing AR chromatin occupancy. RFX6, an AR‐regulated gene linked to rs339331, exhibits synergistic prognostic value for PCa recurrence and metastasis. This comprehensive in vitro and in vivo studies demonstrate the oncogenic functions of RFX6 in promoting PCa cell proliferation and metastasis. Mechanistically, RFX6 upregulates HOXA10 that profoundly correlates with adverse PCa outcomes and is pivotal in RFX6‐mediated PCa progression, facilitating the epithelial‐mesenchymal transition (EMT) and modulating the TGFβ/SMAD signaling axis. Clinically, HOXA10 elevation is associated with increased EMT scores, tumor advancement and PCa recurrence. Remarkably, reducing RFX6 expression restores enzalutamide sensitivity in resistant PCa cells and tumors. This findings reveal a complex interplay of genetic and epigenetic mechanisms in PCa pathogenesis and drug resistance, centered around disrupted prostate lineage AR signaling and abnormal RFX6 expression.
PICALM Regulating the Generation of Amyloid β‐Peptide to Promote Anthracycline‐Induced Cardiotoxicity
Authors: Bao Mengni , Hua Xiumeng , Chen Xiao , An Tao , Mo Han , Sun Zhe , Tao Menghao , Yue Guangxin , Song Jiangping 
Published: June 2024

AbstractAnthracyclines are chemotherapeutic drugs used to treat solid and hematologic malignancies. However, life‐threatening cardiotoxicity, with cardiac dilation and heart failure, is a drawback. A combination of in vivo for single cell/nucleus RNA sequencing and in vitro approaches is used to elucidate the underlying mechanism. Genetic depletion and pharmacological blocking peptides on phosphatidylinositol binding clathrin assembly (PICALM) are used to evaluate the role of PICALM in doxorubicin‐induced cardiotoxicity in vivo. Human heart tissue samples are used for verification. Patients with end‐stage heart failure and chemotherapy‐induced cardiotoxicity have thinner cell membranes compared to healthy controls do. Using the doxorubicin‐induced cardiotoxicity mice model, it is possible to replicate the corresponding phenotype in patients. Cellular changes in doxorubicin‐induced cardiotoxicity in mice, especially in cardiomyocytes, are identified using single cell/nucleus RNA sequencing. Picalm expression is upregulated only in cardiomyocytes with doxorubicin‐induced cardiotoxicity. Amyloid β‐peptide production is also increased after doxorubicin treatment, which leads to a greater increase in the membrane permeability of cardiomyocytes. Genetic depletion and pharmacological blocking peptides on Picalm reduce the generation of amyloid β‐peptide. This alleviates the doxorubicin‐induced cardiotoxicity in vitro and in vivo. In human heart tissue samples of patients with chemotherapy‐induced cardiotoxicity, PICALM, and amyloid β‐peptide are elevated as well.
Biomineralization for Reducing and Controlling Sand‐Dust Storms
Authors: Miao Linchang , Wang Hengxing , Sun Xiaohao , Wu Linyu 
Published: June 2024

AbstractThe sand‐dust weather and sand‐dust storms have become a serious environmental disaster worldwide. It is an important challenge to develop technologies for desert sand solidification in order to prevent and control sand‐dust weather. The biomineralization technology for solidifying desert sands has been a novel method for reinforced soils in recent years. The biomineralization solidification sand field tests are completed at the Wuma Highway solidification section in the Tengger Desert. The superiority of the biomineralization for solidifying sands is verified by measuring the water storage capacity of different reinforcement zones including bare sand zone, plant zone, biomineralization solidifying sand zone, and biomineralization combined plant solidifying sand zone. Simultaneously, the molecular dynamics calculation analysis is used to verify the role of biomineralization solidifying sands in preventing sand‐dust storms. All results demonstrate that the biomineralization solidification sand method is effective for controlling and preventing sandstorm disasters.
Development of a Dual‐Factor Activatable Covalent Targeted Photoacoustic Imaging Probe for Tumor Imaging
Authors: Song Jiho , Zhai Tianqu , Hahm Heung Sik , Li Yuancheng , Mao Hui , Wang Xueding , Jo Janggun , Chang Jae Won 
Published: June 2024

AbstractPhotoacoustic imaging (PAI) is an emerging modality in biomedical imaging with superior imaging depth and specificity. However, PAI still has significant limitations, such as the background noise from endogenous chromophores. To overcome these limitations, we developed a covalent activity‐based PAI probe, NOx‐JS013, targeting NCEH1. NCEH1, a highly expressed and activated serine hydrolase in aggressive cancers, has the potential to be employed for the diagnosis of cancers. We show that NOx‐JS013 labels active NCEH1 in live cells with high selectivity relative to other serine hydrolases. NOx‐JS013 also presents its efficacy as a hypoxia‐responsive imaging probe in live cells. Finally, NOx‐JS013 successfully visualizes aggressive prostate cancer tumors in mouse models of PC3, while being negligibly detected in tumors of non‐aggressive LNCaP mouse models. These findings show that NOx‐JS013 has the potential to be used to develop precision PAI reagents for detecting metastatic progression in various cancers.
High Selectivity MEMS C2H2 Sensor for Transformer Fault Characteristic Gas Detection**
Authors: Xu Yifeng , Mei Haixia , Bing Yu , Zhang Fuyun , Sui Ning , Zhou Tingting , Fan Xiaopeng , Wang Lijie , Zhang Tong 
Published: June 2024

AbstractAcetylene (C2H2), as an important characteristic gas in transformer fault diagnosis, should be accurately detected and effectively distinguished from other dissolved gases (H2, CH4, C2H6, C2H4, CO, CO2), which is crucial to determine whether the fault occurs and the fault type, but also faces challenges now. The rational design and employment of rare earth and noble metals are expected to address this issue. In this work, SnO2‐3 at% Sm2O3‐1 at% PdO based MEMS gas sensor was prepared to achieve high performance detection of C2H2 which has a response value of 56 to 50 ppm C2H2, response/recovery time of 2 s/136 s, lower detection limit of 1 ppm, power consumption of 15.5 mW, and weak cross sensitivity to other transformer fault characteristic gases. Lewis acids and bases theory was used to explain the reason why rare earth Sm is a benefit element to improve selectivity to C2H2. The formation of oxygen vacancies and hetero junctions was used to explain the increased sensitivity of the material. This study proved the feasibility of rare earth and noble metals as potential additives to enable advanced gas‐sensitive materials for highly selective transformer fault characteristic gas C2H2 detection.
Evaluation of operational process parameters of polylactic acid filament extruder by quality and dynamic‐mechanical analysis measurements: A comparative study and classification
Authors: Toprak Esra , Aydin R. Seda Tiğli , Kaya Ceren 
Published: June 2024

AbstractIn this study, the operational conditions of a low‐cost in‐house‐built single screw filament extruder including operational parameters of extruder temperature (170–190°C) and screw speed (20–40 rpm) were evaluated in terms of the quality and dynamic‐mechanical properties of extruded PLA filaments. The designed experimental parameters were used to investigate the impact of operational parameters on filament diameter by Taguchi model where the predicted responses were evaluated. Results showed superior performance with R2 value of 0.9898. The measured filament diameters validated the predicted responses, and the error percentage was in the range of 0%–2.1%. Furthermore, in order to classify and distinguish filaments, we adept the artificial neural network (ANN) model classifier, and the measured dynamic mechanical analysis (DMA) values at certain temperatures were taken into consideration where the classes they belonged to be determined. Results reveal successful classification accuracy of filaments in the range of 94.82%–99.98% depends on different cases of classification process.
Investorsʼ view of mandatory corporate social responsibility as a public policy: The case of section 135 of the Indian Companies Act 2013
Authors: Ruhupatty LeRoy Tim , Ruhupatty Melisa Ann 
Published: June 2024

AbstractWe investigate investor sentiment regarding mandatory corporate social responsibility (CSR) as a public policy. Using the event study methodology, we analysed the cumulative abnormal returns (AR) of companies impacted by Section 135 of the Indian Companies Act 2013. Our findings suggest that setting a CSR expenditure threshold may lead companies lagging in CSR to over‐invest, potentially hindering value maximisation. Specifically, we observed that the cumulative AR for companies lagging in CSR are lower than those leading in CSR. Therefore, mandating CSR practices may be counterproductive for value creation. This event study is one of the first to evaluate the impact of mandatory CSR as public policy on CSR‐leading and lagging firms.
Structural Distortion of g‐C3N4 Induced by a Schiff Base Reaction for Efficient Photocatalytic H2 Evolution
Authors: Yang Chunxia , Cao Hailong , Su Fengyun , Tian Mengzhen , Xie Haiquan , Zhang Yezhen , Jin Xiaoli , Li Xin , Li Zhengdao 
Published: June 2024

AbstractPhotocatalytic H2 evolution by water splitting is a promising approach to address the challenges of environmental pollution and energy scarcity. Graphitic carbon nitride (g‐C3N4) has emerged as a star photocatalyst because of its numerous advantages. To address the limitations of traditional g‐C3N4, namely its inadequate visible light response and rapid recombination of photogenerated carriers, we employed a schiff base reaction to synthesize −C=N− doped g‐C3N4. The introduction of −C=N− groups at the bridging nitrogen sites induced structural distortion in g‐C3N4, facilitating n‐π* electronic transitions from the lone pair electrons of nitrogen atom and extending light absorption up to 600 nm. Moreover, the presence of heterogeneous π‐conjugated electron distribution effectively traps photogenerated electrons and enhances charge carrier separation. Benefiting from its expanded spectral response range, unique electronic properties, increased specific surface area, the doped g‐C3N4 exhibited outstanding photocatalytic H2 evolution performance of 1050.13 μmol/g/h. The value was 5.9 times greater than the pristine g‐C3N4.
Adaptive laboratory evolution in a novel parallel shaken pH‐auxostat
Authors: Sarikaya Burak , Bück Hendrik , Pohen Gino , Rodrigues Filipe , Günster Karsten , Wefelmeier Katrin , Miebach Katharina , Blank Lars M. , Büchs Jochen 
Published: June 2024

AbstractAdaptive laboratory evolution (ALE) is a widely used microbial strain development and optimization method. ALE experiments, to select for faster‐growing strains, are commonly performed as serial batch cultivations in shake flasks, serum bottles, or microtiter plates or as continuous cultivations in bioreactors on a laboratory scale. To combine the advantages of higher throughput in parallel shaken cultures with continuous fermentations for conducting ALE experiments, a new Continuous parallel shaken pH‐auxostat (CPA) was developed. The CPA consists of six autonomous parallel shaken cylindrical reactors, equipped with real‐time pH control of the culture medium. The noninvasive pH measurement and control are realized by biocompatible pH sensor spots and a programmable pump module, to adjust the dilution rate of fresh medium for each reactor separately. Two different strains of the methylotrophic yeast Ogataea polymorpha were used as microbial model systems for parallel chemostat and pH‐auxostat cultivations. During cultivation, the medium is acidified by the microbial activity of the yeast. For pH‐auxostat cultivations, the growth‐dependent acidification triggers the addition of fresh feed medium into the reactors, leading to a pH increase and thereby to the control of the pH to a predetermined set value. By controlling the pH to a predetermined set value, the dilution rate of the continuous cultivation is adjusted to values close to the washout point, in the range of the maximum specific growth rate of the yeast. The pH control was optimized by conducting a step‐response experiment and obtaining tuned PI controller parameters by the Chien‐Hrones‐Reswick (CHR) PID tuning method. Two pH‐auxostat cultivations were performed with two different O. polymorpha strains at high dilution rates for up to 18 days. As a result, up to 4.8‐fold faster‐growing strains were selected. The increased specific maximum growth rates of the selected strains were confirmed in subsequent batch cultivations.
Exploring the Therapeutic Potential of Terpenoids for Depression and Anxiety
Authors: Noor Arif Azimi Md 
Published: June 2024

AbstractThis review focus on the terpenoids as potential therapeutic agents for depression and anxiety disorders, which naturally found in a variety of plants and exhibit a wide range of biological activities. Among the terpenoids discussed in this review are α‐pinene, β‐caryophyllene, α‐phellandrene, limonene, β‐linalool, 1, 8‐cineole, β‐pinene, caryophyllene oxide, p‐cymene, and eugenol. All of these compounds have been studied extensively regarding their pharmacological properties, such as neuroprotective effect, anti‐inflammation, antibacterial, regulation of neurotransmitters and antioxidant effect. Preclinical evidence are reviewed to highlight their diverse mechanisms of action and therapeutic potential to support antidepressant and anxiolytic properties. Additionally, challenges and future directions are also discussed to emphasize therapeutic utility of terpenoids for mental health disorders. Overall, this review provides a promising role of terpenoids as novel therapeutic agents for depression and anxiety, with potential implications for the development of more effective and well‐tolerated treatments in the field of psychopharmacology.
Transport of Zinc‐Phthalocyanine to Cancer Cells Using Myoglobin−Albumin Fusion Protein for Photodynamic Therapy
Authors: Yamada Taiga , Funamoto Mizuki , Takada Ryoya , Morita Yoshitsugu , Komatsu Teruyuki 
Published: June 2024

AbstractPhotodynamic therapy (PDT) is a noninvasive approach to cancer treatment, wherein cell death is initiated by singlet oxygen (1O2) production via energy transfer from excited photosensitizers to ground‐state O2. Effective clinical photosensitizers necessitate water solubility for in vivo administration. Hydrophobic dyes, such as phthalocyanines, cannot be used directly as photosensitizers. Herein, we synthesized a myoglobin‐(human serum albumin) fusion protein reconstituted with zinc‐phthalocyanine (ZnPc), termed ZnPcMb‐HSA. The photophysical properties of ZnPcMb‐HSA closely resemble those of ZnPc‐substituted Mb. Notably, ZnPc dissociates from ZnPcMb‐HSA and selectively accumulates within cancer cells, while the protein components remain extracellular. Treatment of four distinct cell lines with ZnPcMb‐HSA, followed by red‐light irradiation, effectively induced apoptosis. The half‐maximal inhibitory concentrations (IC50) against these cancer cell lines ranged between 0.1–0.5 μM. Reconstituted Mb‐HSA emerges as a promising carrier for transporting various water‐insoluble porphyrinoid photosensitizer to target cancer cells in PDT applications.
Construction of Ni−Re Supported on Hydrotalcite‐Derived MgAl Catalysts for Promoting the Ring Hydrogenation of Furfural into Tetrahydrofurfuryl Alcohol in Water
Authors: Kittisabhorn Aurucha , Ahmed Imtiaz , Ratchahat Sakhon , Chaiwat Weerawut , Koo‐amornpattana Wanida , Klysubun Wantana , Poo‐arporn Yingyot , Limphirat Wanwisa , Khemthong Pongtanawat , Assabumrungrat Suttichai , Srifa Atthapon 
Published: June 2024

AbstractBiomass‐derived feedstocks play a vital role in sustainable economies for renewable energy, waste management, energy security, and commercial prospects. This study focused on promoting the ring hydrogenation of furfural (FAL) into tetrahydrofurfuryl alcohol (THFA) in an aqueous solution using a potential Ni−Re‐supported hydrotalcite‐derived MgAl catalyst at different Ni : Re molar ratios. Structural analyses, such as an in situ time‐resolved X‐ray absorption near edge structure, under H2 reduction elucidated the simultaneous transformation of Re6+ and Re7+ to Re0 with the remaining highly stable Ni2+. The construction of Ni−Re on hydrotalcite‐derived MgAl catalyst resulted in the alleviated H2 reduction behavior, facilitated H2 adsorption and desorption processes, and suitable catalyst acidity detected by H2 temperature‐programmed reduction, and H2 and NH3 temperature‐programmed desorption experiments, respectively. Under optimized conditions, a maximum THFA yield of 78 % with a complete FAL conversion was attained using Ni1Re0.16/MgO−Al2O3 catalyst at a reaction temperature of 140 °C, H2 pressure of 20 bar, catalyst loading of 20 %, and reaction time of 2 h in a H2O system. Isotopic deuterium (D2O) labeling revealed that D substitution was notably pronounced through isotopic exchange between the D atom from D2O and H atom in FAL during its hydrogenation to THFA.
Insights into the High Catalytic Activity of Li‐Ion Battery Waste toward Oxygen Reduction to Hydrogen Peroxide
Authors: Warczak Magdalena , Osial Magdalena , Urbańska Weronika , Sławkowska Natalia , Dąbrowska Agnieszka , Bonarowska Magdalena , Pisarek Marcin , Minikayev Roman , Giersig Michael , Opallo Marcin 
Published: June 2024

AbstractDeveloping highly efficient and cost‐effective electrocatalysts for oxygen reduction reaction (ORR) in aqueous media is crucial for energy conversion systems such as fuel cells or Zn‐air batteries. Electrode materials from spent devices such as lithium‐ion batteries (LiBs) are a serious environmental problem. One of the solutions is their reuse for other electrochemical processes. This work demonstrates the application of solid residues of carbon‐based powders left over from the hydrometallurgical recycling process of LIBs’ waste as efficient catalysts for ORR. Microscopic and spectroscopic studies of the residue disclosed their porous structure and various cobalt contents, depending on the recycling procedure. These battery wastes display ORR catalytic activity when deposited at the liquid‐liquid and solid electrode‐electrolyte interfaces. Scanning Electrochemical Microscopy (SECM) tests showed that assembling battery waste at the liquid‐liquid interface boosts the efficiency of H2O2 production by one to two orders of magnitude. The catalytic activity towards 2‐electron ORR strongly depends on waste powder compositions and structures, e.g., porosity, heteroatom presence, level of defects, and graphitization.
Lyso‐H2S: A Mycophenolic Acid‐Derived Probe for Ultra‐Low Toxicity, Intracellular H2S Detection, and Zebrafish Model Validation
Authors: Jain Neha , An Jongkeol , Roychaudhury Arkaprava , Park Sujeong , Sonawane Prasad M. , Punyamung Pheeranat , Aartsen Lars , Nimse Satish Balasaheb , Kim Cheol‐Hee , Churchill David G. 
Published: June 2024

AbstractIn several biological processes, H2S is known to function as an endogenous gaseous agent. It is very necessary to monitor H2S and relevant physiological processes in vivo. Herein, a new type of fluorophore with a reliable leaving group allows for excited‐state intramolecular transfer characteristics (ESIPT), inspired by mycophenolic acid. A morpholine ring was connected at the maleimide position of the probe to target the lysosome. Subsequently, the dinitrophenyl group known for a photoinduced electron transfer (PET) effect, was connected to allow for an effective “turn‐on” probe Lyso‐H2S. Lyso‐H2S demonstrated strong selectivity towards H2S, a large Stokes shift (111 nm), and an incredibly low detection limit (41.8 nM). The imaging of endogenous and exogenous H2S in living cells (A549 cell line) was successfully achieved because of the specificity and ultra‐low toxicity (100 % cell viability at 50 μM concentration of Lyso‐H2S.) Additionally, Lyso‐H2S was also employed to visualize the activity of H2S in the gallbladder and intestine in a living zebrafish model. This is the first report of a fluorescent probe to track H2S sensing in specific organ systems to our knowledge.
2D Conjugated Polymers Containing B←N Units for Improved Photocatalytic Hydrogen Evolution†
Authors: Zhang Mingcai , Wu Xuan , Zhao Yongqing , Wu Jincai , Pan Xiaobo 
Published: June 2024

Comprehensive SummaryPrevious studies have demonstrated linear polymers embedded with B←N units display efficient photocatalytic hydrogen evolution performance, but their limited structural tunability restricts photogenerated carrier dynamics modulation. Current researches mainly focus on linear polymers, while the study of B←N units in the field of two‐dimension conjugated polymers (2DCPs) photocatalytic hydrogen evolution remains an uncultivated ground. Herein, three 2DCPs containing B←N units were synthesized and their photocatalytic hydrogen production performance was investigated. Among them, the BN‐Tz exhibited the best property with a hydrogen production rate of 89.5 μmol·h–1, while BN‐Ph and BN‐TPB were only 26.4 and 8.5 μmol·h–1, respectively. Comprehensive analyses showed that the remarkable photocatalytic ability of BN‐Tz catalyst was mainly attributed to its superior transport and separation of photogenerated carriers, as well as the ability to construct a stronger built‐in electric field and better planarity. Meanwhile, it was found that the manipulation of the electronic properties of the structures connected to the B←N unit could effectively regulate the molecular polarity, thus achieving the control of the electronic structure of the materials. This work extends the application of materials containing B←N units in photocatalysis and lays a good foundation for the subsequent design of photocatalysts containing B←N units.