bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular and serological surveillance of canine enteric viruses in stray dogs from Vila do Maio, Cape Verde
Authors: Castanheira Pedro , Duarte Ana , Gil Solange , Cartaxeiro Clara , Malta Manuel , Vieira Sara , Tavares Luis 
Published: April 2014

Abstract

Background
Infections caused by canine parvovirus, canine distemper virus and canine coronavirus are an important cause of mortality and morbidity in dogs worldwide. Prior to this study, no information was available concerning the incidence and prevalence of these viruses in Cape Verde archipelago.

Results
To provide information regarding the health status of the canine population in Vila do Maio, Maio Island, Cape Verde, 53 rectal swabs were collected from 53 stray dogs during 2010 and 93 rectal swabs and 88 blood samples were collected from 125 stray dogs in 2011. All rectal swabs (2010 n = 53; 2011 n = 93) were analysed for the presence of canine parvovirus, canine distemper virus and canine coronavirus nucleic acids by quantitative PCR methods. Specific antibodies against canine distemper virus and canine parvovirus were also assessed (2011 n = 88).
From the 2010 sampling, 43.3% (23/53) were positive for canine parvovirus DNA, 11.3% (6/53) for canine distemper virus RNA and 1.9% (1/53) for canine coronavirus RNA. In 2011, the prevalence values for canine parvovirus and canine coronavirus were quite similar to those from the previous year, respectively 44.1% (41/93), and 1.1% (1/93), but canine distemper virus was not detected in any of the samples analysed (0%, 0/93). Antibodies against canine parvovirus were detected in 71.6% (63/88) blood samples and the seroprevalence found for canine distemper virus was 51.1% (45/88).

Conclusions
This study discloses the data obtained in a molecular and serological epidemiological surveillance carried out in urban populations of stray and domestic animals. Virus transmission and spreading occurs easily in large dog populations leading to high mortality rates particularly in unvaccinated susceptible animals. In addition, these animals can act as disease reservoirs for wild animal populations by occasional contact. Identification of susceptible wildlife of Maio Island is of upmost importance to evaluate the risk of pathogen spill over from domestic to wild animals in Cape Verde and to evaluate the associated threat to the wild susceptible species.
Mesoderm is required for coordinated cell movements within zebrafish neural plate in vivo
Authors: Araya Claudio , Tawk Marcel , Girdler Gemma C , Costa Marta , Carmona-Fontaine Carlos , Clarke Jonathan DW 
Published: April 2014

Abstract

Background
Morphogenesis of the zebrafish neural tube requires the coordinated movement of many cells in both time and space. A good example of this is the movement of the cells in the zebrafish neural plate as they converge towards the dorsal midline before internalizing to form a neural keel. How these cells are regulated to ensure that they move together as a coherent tissue is unknown. Previous work in other systems has suggested that the underlying mesoderm may play a role in this process but this has not been shown directly in vivo.

Results
Here we analyze the roles of subjacent mesoderm in the coordination of neural cell movements during convergence of the zebrafish neural plate and neural keel formation. Live imaging demonstrates that the normal highly coordinated movements of neural plate cells are lost in the absence of underlying mesoderm and the movements of internalization and neural tube formation are severely disrupted. Despite this, neuroepithelial polarity develops in the abnormal neural primordium but the resulting tissue architecture is very disorganized.

Conclusions
We show that the movements of cells in the zebrafish neural plate are highly coordinated during the convergence and internalization movements of neurulation. Our results demonstrate that the underlying mesoderm is required for these coordinated cell movements in the zebrafish neural plate in vivo.
Geographic variations and temporal trends in prostate cancer in Martinique over a 25-year period
Authors: Dieye Moustapha , Banydeen Rishika , Macni Jonathan , Michel Stephane , Veronique-Baudin Jacqueline , Sasco Annie , Escarmant Patrick , Joachim Clarisse 
Published: April 2014

Abstract

Background
In Martinique, prostate cancer incidence rates have been increasing since the 1980s and are actually among the highest worldwide. Exposure to lifestyle (changes in dietary habits), environmental factors (exposure to organochlorine pesticides) and modifications in diagnostic and screening procedures, are favored etiological hypotheses. The aim of the present study is to describe and interpret prostate cancer incidence trends over the past 25 years (1981–2005) in Martinique.

Methods
Data on incident prostate cancer cases from 1981 to 2005 were obtained from the population-based Martinique Cancer Registry. World age-standardised incidence rates were calculated and an age-period-cohort model was used to determine average annual variations for prostate cancer during the study period. Age and period effects were assessed, employing the method proposed by Clayton and Schifflers. Relative changes in prostate cancer incidence, at five-year intervals between 1981 and 2005, were also studied with an organochlorine pesticide exposure index, built as a proxy of the relative intensity of chlordecone use on the island between 1973 and 1993.

Results
Prostate cancer incidence was found to increase by 5.07% annually between 1981 and 2005. Compared to 1981–1985, prostate cancer relative risk, in men aged 50–74 years and 75 years and above was respectively 5.98% and 3.07% from 2001 to 2005. An inverse association between population pesticide exposure levels and prostate cancer risk was also highlighted, with highest prostate cancer incidences observed in urban zones showing the lowest soil contamination levels by the chlordecone pesticide (zone 1).

Conclusion
No conclusive association was found between the intensity of pesticide use and the subsequent rise in prostate cancer incidence. However, it remains necessary to develop and reinforce continuous monitoring of prostate cancer incidence and mortality trends on the island. Further studies are also needed in order to consider other risk factors such as modifications in diagnostic and screening procedures over the last 25 years.
Urinary ATP and visualization of intracellular bacteria: a superior diagnostic marker for recurrent UTI in renal transplant recipients?
Authors: Kelley Stephen P , Courtneidge Holly R , Birch Rebecca E , Contreras-Sanz Alberto , Kelly Mark C , Durodie Jerome , Peppiatt-Wildman Claire M , Farmer Christopher K , Delaney Michael P , Malone-Lee James , Harber Mark A , Wildman Scott S 
Published: April 2014

Abstract
Renal transplant recipients (RTR) are highly susceptible to urinary tract infections (UTIs) with over 50% of patients having at least one UTI within the first year. Yet it is generally acknowledged that there is considerable insensitivity and inaccuracy in routine urinalysis when screening for UTIs. Thus a large number of transplant patients with genuine urine infections may go undiagnosed and develop chronic recalcitrant infections, which can be associated with graft loss and morbidity. Given a recent study demonstrating ATP is released by urothelial cells in response to bacteria exposure, possibly acting at metabotropic P2Y receptors mediating a proinflammatory response, we have investigated alternative, and possibly more appropriate, urinalysis techniques in a cohort of RTRs.
Mid-stream urine (MSU) samples were collected from 53 outpatient RTRs. Conventional leukocyte esterase and nitrite dipstick tests, and microscopic pyuria counts (in 1 μl), ATP concentration measurements, and identification of intracellular bacteria in shed urothelial cells, were performed on fresh unspun samples and compared to ‘gold-standard’ bacterial culture results.
Of the 53 RTRs, 22% were deemed to have a UTI by ‘gold-standard’ conventional bacteria culture, whereas 87%, 8% and 4% showed evidence of UTIs according to leukocyte esterase dipstick, nitrite dipstick, and a combination of both dipsticks, respectively. Intracellular bacteria were visualized in shed urothelial cells of 44% of RTRs, however only 1 of the 23 RTRs (44%) was deemed to have a UTI by conventional bacteria culture. A significant association of the ‘gold-standard’ test with urinary ATP concentration combined with visualization of intracellular bacteria in shed urothelial cells was determined using the Fisher’s exact test.
It is apparent that standard bedside tests for UTIs give variable results and that seemingly quiescent bacteria in urothelial cells are very common in RTRs and may represent a focus of subclinical infection. Furthermore, our results suggest urinary ATP concentration combined with detection of intracellular bacteria in shed urinary epithelial cells may be a sensitive means by which to detect ‘occult’ infection in RTRs.
Antiphospholipid syndrome in 2014: more clinical manifestations, novel pathogenic players and emerging biomarkers
Authors: Meroni Pier Luigi , Chighizola Cecilia Beatrice , Rovelli Francesca , Gerosa Maria 
Published: April 2014

AbstractThe clinical spectrum of the anti-phospholipid syndrome (APS) is not limited to vascular thrombosis or miscarriages but includes additional manifestations that cannot be explained solely by a thrombophilic state. Anti-cardiolipin, anti-beta2 glycoprotein I (anti-β2GPI) and lupus anticoagulant (LA) assays are not only the formal diagnostic and classification laboratory tools but also parameters to stratify the risk to develop the clinical manifestations of the syndrome. In particular, anti-β2GPI antibodies reacting with an immunodominant epitope on domain I of the molecule were reported as the prevalent specificity in APS patients, correlating with a more aggressive clinical picture. Several laboratory assays to improve the diagnostic and predictive power of the standard tests have been proposed. Plates coated with the phosphatidylserine-prothrombin complex for detecting antibodies represent a promising laboratory tool correlating with LA and with clinical manifestations. Anti-phospholipid antibodies can be found in patients with full-blown APS, in those with thrombotic events or obstetric complications only or in asymptomatic carriers. An inflammatory second hit is required to increase the presence of β2GPI in vascular tissues, eventually triggering thrombosis. Post-transcriptional modifications of circulating β2GPI, different epitope specificities or diverse anti-β2GPI antibody-induced cell signaling have all been suggested to affect the clinical manifestations and/or to modulate their occurrence.
The Risk of Osteonecrosis of the Jaws in Taiwanese Osteoporotic Patients Treated With Oral Alendronate or Raloxifene
Authors: Chiu Wei-Yih , Chien Jung-Yien , Yang Wei-Shiung , Juang Jyh-Ming Jimmy , Lee Jang-Jaer , Tsai Keh-Sung 
Published: April 2014

Background:
This study aimed to explore the possible association between osteonecrosis of the jaws (ONJ) and oral alendronate or raloxifene used for osteoporosis and to estimate its absolute and attributable risks in the Taiwanese population.

Methods:
Using an electronic medical records system and manual confirmation of ONJ, we identified patients who began taking alendronate or raloxifene for osteoporosis and developed ONJ between January 2000 and April 2012.

Results:
The incidence of ONJ associated with oral alendronate for the management of osteoporosis began after 1 year of drug exposure and progressively increased with longer durations of therapy, specifically from 0.23% to 0.92% as the duration of treatment went from 2 years to 10 years. The overall frequency of ONJ related to oral alendronate over a 12-year period was 0.55%. The incidence rate of ONJ attributed to alendronate exposure was 283 per 100 000 persons per year. On multivariate Cox proportional analysis, adjusting for the potential confounders, alendronate remains an independent predictor for ONJ occurrence [hazard ratio 7.42 (1.02–54.09)] compared with raloxifene. Advanced age, drug duration, and coexisting diabetes and rheumatoid arthritis are contributing factors to the development of oral alendronate-related ONJ.

Conclusion:
We provided the evidence to support the association of ONJ with oral alendronate used in the treatment or prevention of osteoporosis.
Estimating Demand for Mobile Applications in the New Economy
Authors: Ghose Anindya , Han Sang Pil 
Published: April 2014

In 2013, the global mobile app market was estimated at over US$50 billion and is expected to grow to $150 billion in the next two years. In this paper, we build a structural econometric model to quantify the vibrant platform competition between mobile (smartphone and tablet) apps on the Apple iOS and Google Android platforms and estimate consumer preferences toward different mobile app characteristics. We find that app demand increases with the in-app purchase option wherein a user can complete transactions within the app. On the contrary, app demand decreases with the in-app advertisement option where consumers are shown ads while they are engaging with the app. The direct effects on app revenue from the inclusion of an in-app purchase option and an in-app advertisement option are equivalent to offering a 28% price discount and increasing the price by 8%, respectively. We also find that a price discount strategy results in a greater increase of app demand in Google Play compared with Apple App Store, and app developers can maximize their revenue by providing a 50% discount on their paid apps. Using the estimated demand function, we find that mobile apps have enhanced consumer surplus by approximately $33.6 billion annually in the United States, and we discuss various implications for mobile marketing analytics, app pricing, and app design strategies.This paper was accepted by Alok Gupta, special issue on business analytics.
Combinatorial Benders' Cuts for the Strip Packing Problem
Authors: Côté Jean-François , Dell'Amico Mauro , Iori Manuel 
Published: April 2014

We study the strip packing problem, in which a set of two-dimensional rectangular items has to be packed in a rectangular strip of fixed width and infinite height, with the aim of minimizing the height used. The problem is important because it models a large number of real-world applications, including cutting operations where stocks of materials such as paper or wood come in large rolls and have to be cut with minimum waste, scheduling problems in which tasks require a contiguous subset of identical resources, and container loading problems arising in the transportation of items that cannot be stacked one over the other. The strip packing problem has been attacked in the literature with several heuristic and exact algorithms, nevertheless, benchmark instances of small size remain unsolved to proven optimality. In this paper we propose a new exact method that solves a large number of the open benchmark instances within a limited computational effort. Our method is based on a Benders' decomposition, in which in the master we cut items into unit-width slices and pack them contiguously in the strip, and in the slave we attempt to reconstruct the rectangular items by fixing the vertical positions of their unit-width slices. If the slave proves that the reconstruction of the items is not possible, then a cut is added to the master, and the algorithm is reiterated. We show that both the master and the slave are strongly 𝒩𝒫-hard problems and solve them with tailored preprocessing, lower and upper bounding techniques, and exact algorithms. We also propose several new techniques to improve the standard Benders' cuts, using the so-called combinatorial Benders' cuts, and an additional lifting procedure. Extensive computational tests show that the proposed algorithm provides a substantial breakthrough with respect to previously published algorithms.
10% Tumor Diameter Shrinkage on the First Follow-Up Computed Tomography Predicts Clinical Outcome in Patients With Advanced Renal Cell Carcinoma Treated With Angiogenesis Inhibitors: A Follow-Up Validation Study
Authors: Krajewski Katherine M. , Franchetti Yoko , Nishino Mizuki , Fay André P. , Ramaiya Nikhil , Van den Abbeele Annick D. , Choueiri Toni K. 
Published: April 2014

Abstract

Background.
Vascular endothelial growth factor (VEGF)-targeted agents are standard therapies for metastatic renal cell carcinoma (mRCC), associated with variable tumor shrinkage. Response Evaluation Criteria in Solid Tumors (RECIST) is of limited utility in this setting, and other imaging changes are sought to reliably predict outcome early. We aim to validate 10% tumor shrinkage as the best early indicator of outcome.

Methods.
In this institutional review board-approved, Health Insurance Portability and Accountability Act-compliant study, 66 mRCC patients with 165 lesions on clinical trials of VEGF-targeted agents underwent thoracic and abdominal computed tomography at baseline and at first follow-up after therapy. Measurements were performed according to RECIST and tumor shrinkage of ≥10% decrease in sum of the longest diameter (−10%SLD). Correlation with time-to-treatment failure (TTF) and overall survival (OS) were compared and stratified by response to the radiologic criteria. Receiver-operating characteristics (ROC) analysis yielded the optimal threshold change in SLD, defining patients with prolonged survival.

Results.
More than −10%SLD significantly differentiated responders from nonresponders (median TTF 8.4 vs. 4.1 months, p = .001), whereas partial response by RECIST did not (median TTF 6.9 vs. 5.5 months in responders vs. nonresponders, p = .34). −10%SLD was also significantly predictive of OS (median OS 35.1 vs. 15.0 months in responders vs. nonresponders, p = .003). ROC curve analysis yielded −9.3% in SLD as the optimal threshold for response/no response.

Conclusion.
Ten percent tumor shrinkage is validated as a reliable early predictor of outcome in mRCC patients receiving VEGF-targeted therapies and may provide a practical measure to guide therapeutic decisions.
Frequency of Morning Ketosis After Overnight Insulin Suspension Using an Automated Nocturnal Predictive Low Glucose Suspend System
Authors: Beck Roy W. , Raghinaru Dan , Wadwa R. Paul , Chase H. Peter , Maahs David M. , Buckingham Bruce A. ,   for the In Home Closed Loop Study Group
Published: April 2014

OBJECTIVE
To assess the effect of overnight insulin pump suspension in an automated predictive low glucose suspend system on morning blood glucose and ketone levels in an attempt to determine whether routine measurement of ketone levels is useful when a closed-loop system that suspends insulin delivery overnight is being used.

RESEARCH DESIGN AND METHODS
Data from an in-home randomized trial of 45 individuals with type 1 diabetes (age range 15–45 years) were analyzed, evaluating an automated predictive low glucose pump suspension system in which blood glucose, blood ketone, and urine ketone levels were measured on 1,954 mornings.

RESULTS
One or more pump suspensions occurred during 744 of the 977 intervention nights (76%). The morning blood ketone level was ≥0.6 mmol/L after 11 of the 744 nights (1.5%) during which a pump suspension occurred and 2 of the 233 nights (0.9%) during which there was no suspension compared with 11 of 977 control nights (1.1%). The morning blood ketone level was ≥0.6 mmol/L after only 2 of 159 nights (1.3%) with a pump suspension exceeding 2 h. Morning fasting blood glucose level was not a good predictor of the presence of blood ketones.

CONCLUSIONS
Routine measurement of blood or urine ketones during use of an automated pump suspension system using continuous glucose monitoring, whether threshold based or predictive, is not necessary. Recommendations for checking ketone levels should be no different when a patient is using a system with automated insulin suspension than it is for conventional diabetes self-management.
Ventriloquist Theatre and the Omniscient Narrator: Gatz and El pasado es un animal grotesco
Authors: Fuchs Barbara 
Published: April 2014

ABSTRACT: By replacing dialogue with narration, ventriloquist theatre raises central questions about its ethical and political costs, while challenging our understanding of the limits between drama and narrative. In very different ways, Elevator Repair Service’s Gatz (first performed 2006) and Mariano Pensotti’s El pasado es un animal grotesco [The Past Is a Grotesque Animal] (2010) redraw the boundaries between literary modes, putting characters onstage but replacing their dialogue with narrated text. Staging narration allows us to reconsider literary mechanisms to which we have largely become inured. Rendering the invisible narrator as a presence on the stage lays bare both the arbitrariness of narrative authority and its costs.
Helicopter-borne radar imaging of snow cover on and around glaciers in Alaska
Authors: Gusmeroli Alessio , Wolken Gabriel J. , Arendt Anthony A. 
Published: April 2014

AbstractDuring spring 2013, we performed 500 MHz, helicopter-borne impulsive ground-penetrating radar surveys of several glaciers and glacier forelands in south-central Alaska, USA. These surveys were designed to obtain spatially distributed measurements of snow accumulation spanning a broad range of continental and maritime climatic zones. Visual assessment of radar images shows that data quality varied with the terrains and was optimal for snow that covered smooth glacier ice and firn, smooth debris-covered areas and moraines, freshwater lake and river ice, tundra, and taiga. Conversely, returns from the base of the snowpack were unrecognizable over rough debris-covered glacier termini, icefalls and some high-altitude accumulation basins. Optimal flying speed was 15-20ms–1 (30–40kt). At these speeds, which are two to three times faster than previously reported for such surveys, we could still identify snow-depth data with confidence, at a point spacing of ~1.5-2.0m. Data quality on glaciers decreased with increased air speed, though useful echoes from the base of the snowpack were still obtained at 40-45 ms–1 (87 kt; data point spacing of 6-8 m). Similar high-speed surveys over non-glacial terrains were unsuccessful, as basal reflections were no longer recognizable.
Automated mapping of local layer slope and tracing of internal layers in radio echograms
Authors: Panton Christian 
Published: April 2014

AbstractAn automated method is presented for tracing layers in radio echograms. The method is designed to work with most radio-echo sounding echograms and has been successfully tested with a 180–210 MHz multichannel coherent depth sounder. To accurately trace layers, first approximate layer positions are calculated by integrating the local layer slope which is inferred by the intensity response to a slanted filter, then the positions are refined using an iterative optimization. The layers are traced using an active contour model or snake, which can be constrained to conserve both echogram features and smooth layers. With this technique it is possible to trace internal layers over distances of several hundred kilometers. The method was tested between two Greenland deep ice cores where the age–depth relation is known.