bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermostable lipase fromGeobacillussp. Iso5: Bioseparation, characterization and native structural studies
Authors: Mahadevan Gurumurthy D. , Neelagund Shivayogeeswar E 
Published: June 2013

AbstractThe extracellular thermoalkaline lipase fromGeobacillussp. Iso5 was purified to homogeneity by ultrafiltration, 6% cross‐linked agarose and Phenyl spehrose HIC column chromatography. The final purified lipase resulted in 8.7‐fold with 6.2% yield. The relative molecular weight of the enzyme was determined to be a monomer of 47 kDa by SDS–PAGE and MALDI‐TOF MS/MS spectroscopy. The purified enzyme exhibit optimum activity at 70 °C and pH 8.0. The enzyme retained above 90% activity at temperatures of 70 °C and about 35% activity at 85 °C for 2 h. However, the stability of the enzyme decreased at the temperature over 90 °C. The enzyme activity was promoted in the presence of Ca2+and Mg2+and strongly inhibited by HgCl2, PMSF, DTT, K+, Co2+, and Zn2+. EDTA did not affect the enzyme activity. The secondary structure of purified lipase contains 36% α‐helix and 64% β‐sheet which was determined by Circular dichromism, FTIR, and Raman Spectroscopy.
Internal pressure of human meniscal root attachments during loading
Authors: Abraham Adam C. , Villegas Diego F. , Kaufman Kenton R. , Haut Donahue Tammy L. 
Published: June 2013

ABSTRACTThis study investigated the internal fluid pressure of human cadaver meniscal root attachments. A pressure micro‐sensor was implanted inside each attachment site. Tibiofemoral joints were compressed to 2× body weight at various flexion angles and pressure recorded for 20 min. The anterior cruciate ligament (ACL) was then transected and joints retested. Lastly, a longitudinal incision of the lateral posterior (LP) horn was made and the joint retested. Ramp pressure was defined as the pressure when 2× body weight was reached, and equilibrium pressure was recorded at the end of the hold period. The medial posterior (MP) attachment was subjected to greater ramp pressure than the medial anterior (p = 0.002) and greater equilibrium pressure than all other root attachment sites (p < 0.001). Flexion angle had a significant effect on pressure as full extension was greatest at ramp (p = 0.040). Transection of the ACL decreased ramp pressure in the LP attachment (p = 0.025) and increased equilibrium pressure (p = 0.031) in the MP attachment. The results suggest that repair strategies should be developed which reconstruct the MP attachments to be sufficient to withstand large pressures. Furthermore, since meniscal pressure is highest at full extension, this fact should be considered when prescribing rehabilitation following repair of an attachment. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1507–1513, 2013
Residual effects of organic Zn fertilizers applied before the previous crop on Zn availability and Zn uptake by flax (Linum usitatissium)
Authors: Almendros Patricia , Gonzalez Demetrio , Alvarez Jose M. 
Published: June 2013

AbstractThe objective of this study was to compare the residual effect of zinc (Zn) from three Zn chelates (Zn‐aminelignosulfonate, Zn‐AML; Zn‐polyhydroxyphenylcarboxylate, Zn‐PHP; and Zn‐ethylenediaminedisuccinate, Zn‐EDDS), applied at two rates (5 and 10 mg Zn [kg soil]–1, respectively) to a previous crop, for a flax crop (Linum usitatissimum L.). For the greenhouse experiment, two different soils were used: a weakly acidic soil, classified as Typic Haploxeralf (Soilacid), and a calcareous soil, classified as Typic Calcixerept (Soilcalc). Plant availability of soil Zn was evaluated using the DTPA‐triethanolamine (TEA), Mehlich 3, and low‐molecular‐weight organic acids (LMWOAs) methods. Easily leachable Zn was determined, and soil Zn status was characterized based on the Zn distribution in different fractions obtained by a sequential extraction. The Zn reserves after the previous crop were substantial and ranged from 2.85% to 5.61% of available Zn (Mehlich 3‐extractable) with respect to the applied Zn. Plant parameters such as dry‐matter yield, total Zn, and soluble Zn concentrations were measured, and Zn utilization by plants was calculated. In both soils, the highest concentrations of available Zn were associated with the application of Zn‐AML at a rate of 10 mg Zn kg–1. In Soilacid the largest quantity of easily leachable Zn was also observed with Zn‐AML fertilizer. Similarly, Zn‐AML resulted in the highest Zn concentration in flax seeds (229 mg Zn kg–1 and 72 mg Zn kg–1 for the highest rate of Zn application to Soilacid and Soilcalc, respectively). The results suggest that these Zn chelates resulted in a residual effect in soils with appropriate concentrations of the most labile fractions of Zn and available Zn, particularly when Zn‐AML was applied at the highest rate. This chelate was more effective in Soilacid than in Soilcalc. In the weakly acidic soil at the lowest Zn level it was associated with the highest percentage of Zn utilization by the flax plant and the most effective Zn transfer from soil to the plant.
Long‐term outcomes for the endoscopic modified lothrop/draf III procedure: A 10‐year review
Authors: Naidoo Yuresh , Bassiouni Ahmed , Keen Mark , Wormald Peter J. 
Published: June 2013

Objectives/HypothesisTo detail the long‐term outcomes of the endoscopic modified Lothrop procedure (EMLP) (also know as Draf III/frontal drillout) and identify key risk factors for failure.Study DesignRetrospective cohort study and chart review.MethodsEndoscopic assessment of frontal ostium patency and patient‐reported symptoms were prospectively collected on patients who underwent EMLP between January 2001 and December 2011 for chronic rhinosinusitis (CRS). Risk factors for failing EMLP were identified.ResultsThere were 229 patients who met the inclusion and exclusion criteria and underwent an EMLP. The average number of standard endoscopic sinus surgery procedures prior to an EMLP was 3.8 (95% confidence interval [CI]: 3.4‐4.2, standard deviation [SD]: 3.3).The average length of follow‐up was 45.0 months (95% CI: 41.2–48.9 months, SD: 22.3 months). The EMLP was successful in 95% (217/229), with no further surgery being required. Postsurgical recurrence of disease with persistence of symptoms requiring revision EMLP occurred in 12 patients. No complications were identified. Allergic fungal sinusitis and recurrent Staphylococcus aureus infections were identified as potential risk factors for failure.ConclusionsThis is the single largest study of EMLP in the literature with a long follow‐up period. It illustrates the benefit of the EMLP for patients with CRS recalcitrant to medical and standard endoscopic sinus surgery.Level of Evidence4 Laryngoscope, 124:43–49, 2014
A Hybrid Particle‐Field Coarse‐Grained Molecular Model for Pluronics Water Mixtures
Authors: De Nicola Antonio , Kawakatsu Toshihiro , Milano Giuseppe 
Published: June 2013

AbstractIn the framework of a hybrid particle‐field simulation technique where self‐consistent field (SCF) theory and molecular dynamics (MD) are combined, specific coarse‐grained (CG) models for Pluronic block copolymers are developed. In particular, the behavior of the model in the correct reproduction of micellar and non‐micellar phases has been tested for Pluronic L62 and L64. At different temperature and polymer contents of the water–polymer mixture, the proposed model is able to correctly describe the different morphologies that are experimentally found. The proposed CG models, still very close to atomistic ones, allow the reconstruction of full atomistic structures by applying suitable reverse mapping techniques. This opens the way to all atom description of these systems.magnified image
Störungsfrei mittels optischem Multiplexing
Authors: Krüger Sven , Nedvidek Francis 
Published: June 2013

AbstractDie stetig steigende Flut des weltweiten mobilen Datenverkehrs führt direkt zu Engpässen in den nachgeschalteten fasergebundenen Metronetzen: Aufgrund der hohen Datenraten kann der Mobilfunkdatenverkehr vor allem in Ballungsräumen nicht mehr über Richtfunk vom Handymast zu einem zentralen „Sammelpunkt”︁ abgeführt werden, sondern wird direkt am Mast auf eine Glasfaserstrecke übergeben.
Miniaturisierungspotenziale in der Raumfahrt
Authors: Liebold Florian , Hanbuch Karsten 
Published: June 2013

AbstractRegelmäßig sorgen spektakuläre Satellitenbilder für Verblüffung. Kommunikations‐ und Navigationssatelliten erleichtern unser aller Leben. Und Weltraumteleskope sind unsere Augen und Ohren im All. Doch es sind nicht nur tonnenschwere Raumfahrtzeuge, die uns mit Informationen beliefern. So kommen auch Satellitensysteme in der Größe von handelsüblichen Kühlschränken als Träger hochpräziser Instrumente zum Einsatz: sogenannte Mikro‐Satelliten (Abb. 1). Die für die Lageregelung solcher Satelliten unabdingbaren Drehraten‐Sensoren erreichen mittlerweile einen sehr hohen Miniaturisierungsgrad — Dank optischer Technologien.
Urologic co‐morbidities associated with sacrococcygeal teratoma and a rational plan for urologic surveillance
Authors: Cost Nicholas G. , Geller James I. , Le Louis D. , Crombleholme Timothy M. , Keswani Sundeep G. , Lim Foong‐Yen , Alam Shumyle 
Published: June 2013

ABSTRACTBackgroundSacrococcygeal teratoma (SCT) is one of the most common neonatal and fetal tumors. SCT pelvic mass effect and the need for aggressive surgical resection, create potential for urologic co‐morbidity. We reviewed our experience with SCTs and propose a rational plan for urologic surveillance.MethodsWe retrospectively reviewed all patients with SCT evaluated at our institution from 2004 to 2011. We collected data on the need for reconstructive surgery related to the urologic co‐morbidity, the time to detection of urologic co‐morbidity, and length of follow‐up.ResultsWe identified 28 patients evaluated during the study period with a median follow‐up of 3.1 year (range 0.14–13.4). The Altman classifications were—type I: 7 (25%), II: 15 (53.6%), and III: 6 (21.4%). Eighteen (64.3%) patients had an associated urologic co‐morbidity: 12 (42.9%) patients had hydronephrosis, VUR—10 (35.7%), NGB—13 (46.4%), and 4 (14.3%) developed ≥CKD2. When comparing the patients according to Altman classification, there was a trend towards more urologic co‐morbidity in patients with increasing pelvic involvement, P = 0.06. Eleven patients (39.3%) had delayed urologic evaluation and five (17.9%) required reconstructive urologic surgery. In comparing these groups, 4 of 11 (36.4%) undergoing delayed urologic evaluation progressed to reconstruction, as opposed to only one of 17 (5.7%) with urologic evaluation within first year of life (P‐value = 0.06).ConclusionUrologic co‐morbidities are common in children with SCT and appear most common in patients with more pelvic tumor involvement (≥Altman II). A risk‐adapted approach to urologic surveillance is proposed. Pediatr Blood Cancer 2013;60:1626–1629. © 2013 Wiley Periodicals, Inc.
Risk factors for hyperferritinemia secondary to red blood cell transfusions in pediatric cancer patients
Authors: Amid Ali , Barrowman Nick , Vijenthira Abi , Lesser Pat , Mandel Karen , Ramphal Raveena 
Published: June 2013

AbstractBackgroundTransfusion of packed red blood cells is common in pediatric cancer patients who receive chemotherapy. This study was done to identify characteristics of pediatric cancer patients at risk of hyperferritinemia secondary to frequent transfusions.ProcedureIn this retrospective chart review, all pediatric cancer patients who completed chemotherapy from January 2007 to January 2012 and had an assessment of serum ferritin 6 months after the end of treatment were included. Variables included: age, sex, type of cancer diagnosis, weight and body surface area (BSA) at the time of diagnosis, number of transfusions, total transfused volume (TTV), total transfused volume per body weight (TVPBW), and weight and BSA change from the time of diagnosis to the time of ferritin check.ResultsOf 109 eligible patients, 85 (78%) received transfusions. Sixteen patients (14.7%) had ferritin levels > 200 µg/L and four (3.7%) had ferritin levels > 1,000 µg/L. Although age, weight and BSA at cancer diagnosis, number of transfusions and TVPBW were correlated with the level of ferritin, independent risk factors were TTV (range 1,961–30,090 ml in patients with hyperferritinemia, P < 0.001) and BSA change from the time of diagnosis to the time of ferritin check (range −0.15 to 0.31 m2 in patients with hyperferritinemia, P < 0.001). Increase in BSA was correlated with reduction of hyperferritinemia in follow‐up ferritin measurements (P = 0.049).ConclusionsIn addition to TTV, change in BSA is an independent predictor for the degree and possibly persistence of hyperferritinemia in pediatric cancer patients and should be considered in decisions to initiate interventions. Pediatr Blood Cancer 2013;60:1671–1675. © 2013 Wiley Periodicals, Inc.
Spiritual beliefs near the end of life: a prospective cohort study of people with cancer receiving palliative care
Authors: King Michael , Llewellyn Henry , Leurent Baptiste , Owen Faye , Leavey Gerard , Tookman Adrian , Jones Louise 
Published: June 2013

AbstractObjectivesDespite growing research interest in spirituality and health, and recommendations on the importance of spiritual care in advanced cancer and palliative care, relationships between spiritual belief and psychological health near death remain unclear. We investigated (i) relationships between strength of spiritual beliefs and anxiety and depression, intake of psychotropic/analgesic medications and survival in patients with advanced disease; and (ii) whether the strength of spiritual belief changes as death approaches.MethodsWe conducted a prospective cohort study of 170 patients receiving palliative care at home, 97% of whom had a diagnosis of advanced cancer. Data on strength of spiritual beliefs (Beliefs and Values Scale [BVS]), anxiety and depression (Hospital Anxiety and Depression Scale [HADS]), psychotropic/analgesic medications, daily functioning, global health and social support were collected at recruitment then 3 and 10 weeks later. Mortality data were collected up to 34 months after the first patient was recruited.ResultsRegression analysis showed a slight increase in strength of spiritual belief over time approaching statistical significance (+0.16 BVS points per week, 95% CI [−0.01, 0.33], p = 0.073). Belief was unrelated to anxiety and depression (−0.15 points decrease in HADS for 10 points increased in BVS (95% CI [−0.57, 0.27], p = 0.49) or consumption of psychotropic medication). There was a non‐significant trend for decreasing analgesic prescription with increasing belief. Mortality was higher over 6 months in participants with lower belief at recruitment.ConclusionResults suggest that although religious and spiritual beliefs might increase marginally as death approaches, they do not affect levels of anxiety or depression in patients with advanced cancer. © 2013 The Authors. Psycho‐Oncology published by John Wiley & Sons, Ltd.
The effect of electrode/graphene interfaces and dephasing processes on conductance and giant magnetoresistance
Authors: Krompiewski S. 
Published: June 2013

AbstractThis study is devoted to potential applications of graphene nanostructures (GNRs) in future electronics and spintronics. This problem is related with the impact of geometrical and magnetic features of interfaces between contacts and the GNR on electrical performance of the device. The present approach combines the tight‐binding model and Green's function technique. It makes possible to put end‐contacted and side‐contacted arrangements on an equal footing, and understand their effect on conductance and giant magnetoresistance (GMR), depending on: aspect ratio, current orientation, gate voltage and dephasing. It turns out that at low gate voltages, dephasing processes may enhance the GMR effect, and that for short nanoribbons the end‐contacted geometry leads to the highest conductance. Long semiconducting armchair graphene nanoribbons, in turn, may reveal a sizeable inverse GMR effect. Some additional insight into the scalability of GNRs, for the spin‐dependent transport, is also provided.magnified imageEnd‐contacted zigzag GNR with infinite source and drain electrodes (top and bottom) and a fictitious dephasing contact (left).(© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A simple solution method for the blade element momentum equations with guaranteed convergence
Authors: Ning S. Andrew 
Published: June 2013

ABSTRACTThe blade element momentum (BEM) equations, though conceptually simple, can be challenging to solve reliably and efficiently with high precision. These requirements are particularly important for efficient rotor blade optimization that utilizes gradient‐based algorithms. Many solution approaches exist for numerically converging the axial and tangential induction factors. These methods all generally suffer from a lack of robustness in some regions of the rotor blade design space, or require significantly increased complexity to promote convergence. The approach described here allows for the BEM equations to be parameterized by one variable—the local inflow angle. This reduction is mathematically equivalent, but greatly simplifies the solution approach. Namely, it allows for the use of one‐dimensional root‐finding algorithms for which very robust and efficient algorithms exist. This paper also discusses an appropriate arrangement of the equation and corresponding bounds for the one‐dimensional search—intervals that bracket the solution and over which the function is well behaved. The result is a methodology for solving the BEM equations with guaranteed convergence and at a superlinear rate.Copyright © 2013 John Wiley & Sons, Ltd.
Charge Density Analysis of an Organic Ferroelectric. Croconic Acid: an Experimental and Theoretical Study
Authors: Zhurov Vladimir V. , Pinkerton A. Alan 
Published: June 2013

AbstractThe charge density in the high density (ρ= 1.912 g·cm–3) organic ferroelectric, croconic acid, was determined from low temperature (20 K) X‐ray diffraction data, and DFT theoretical calculations. The high density was associated with strong intermolecular interaction energies. The spontaneous polarization is correlated with the derived molecular dipole moment (9.3 Debye). Improvements in multipole refinement protocols are described. Limitations in the multipole model with respect to heteronuclear bond properties are discussed.
Synthesis, Structures and DFT Studies of Imido‐bridged and (Bis)ligand‐coordinated Titanium Complexes
Authors: Wu Jian , Chen Yanmei , Chen Zhou , Liu Wei , Li Yahong , Pei Hao , Liu Yonglu , Gao Qian , Li Wu 
Published: June 2013

AbstractSyntheses and structures of five imido‐bridged dinuclear titanium complexes and two (bis)ligand‐coordinated mononuclear titanium complexes are reported. Addition of 1 or 2 equiv. of Schiff base ligand (((1H‐pyrrol‐2‐yl)methylene)amino)‐2,3‐dihydro‐1H‐inden‐2‐ol (H2L) to Ti(NMe2)4 resulted in transamination with 4 equiv. of dimethylamides generating a (bis)ligand‐coordinated complex Ti(L)2 (1). Treatment of Ti(NMe2)4 with 1 equiv. of tBuNH2 followed by addition of 1 equiv. of H2L afforded an imido‐bridged complex [Ti(L)(NtBu)]2 (2). 1:1:1:1 reaction of Ti(NMe2)4/RNH2/H2L/py(or phen) produced imido‐bridgedcomplexes [Ti(L)(NPh)(py)]2 (3), [Ti(L)(4‐F‐PhN)(py)]2·Tol (4·Tol), [Ti(L)(4‐Cl‐PhN)(py)]2·Tol·THF (5·Tol·THF), [Ti(L)(4‐Br‐PhN)(py)]2·Tol (6·Tol) and a (bis)ligand‐coordinated complex Ti(L)2·phen (7) (py = pyridine, phen = 1,10‐phenanthroline). Attempts to prepare the monomeric titianium imido complexes were unsuccessful. DFT studies show that the assumed compound which contains Ti = N species is less stable than imido‐bridged Ti‐N(R)‐Ti complexes, providing the better understanding of the experimental results.
Surf Lifeguard Rescues
Authors: Morgan Damian , Ozanne-Smith Joan 
Published: June 2013

Objective This study assessed the utility of lifeguard rescue data for providing information on person and situation factors to inform surf bather drowning prevention research. Methods The dataset comprised 872 beach-days (daily lifeguard reports) obtained from 26 beaches over a 95-day period in Victoria, Australia. Results The rescue rate was 128 per 100,000 in-water bathers. One or more rescues were required on 125 beach-days (14%). Rescue on a beach-day was more likely for offshore wind conditions, relatively high daily air temperatures, and high bather numbers ( P < .05). Compared to female bathers, males were more frequently rescued (65%) and more likely ( P < .05) to be from a younger age group (30 years or less), although being older was associated with a relatively poorer condition on rescue. Conclusions Although rescues are proportional to water exposure, frequencies are also influenced by situation and person factors. Bathers at relatively high risk of rescue are hypothesized to be overrepresented in amenable sea and weather conditions, and poor patient condition on rescue may be associated with exposure to a preexisting health condition.
Air entrainment during impact of droplets on liquid surfaces
Authors: Tran Tuan , de Maleprade Hélène , Sun Chao , Lohse Detlef 
Published: June 2013

AbstractWe study drop impact on a deep pool of the same fluid, with an emphasis on the air layer trapped under the droplets from its formation to its rupture. The penetration velocity of the air layer at a very short time scale prior to its rupture is shown, using an energy argument and experimental verification, to be one-half of the impact velocity. We then deduce the dependence of the rupture position on the liquid viscosity and the impact velocity. We show that the volume of the resulting air bubbles can be related to both those resulting from droplets impacting on solid surfaces and those resulting from rigid spheres impacting on liquid surfaces.
Evaluation of acetylcholinesterase activity and behavioural alterations induced by ketamine in an animal model of schizophrenia
Authors: Zugno Alexandra I. , Matos Maria Paula , Canever Leila , Fraga Daiane B. , De Luca Renata D. , Ghedim Fernando V. , Deroza Pedro F. , de Oliveira Mariana B. , Pacheco Felipe D. , Valvassori Samira S. , Volpato Ana Maria , Budni Josiane , Quevedo João 
Published: June 2013

ObjectiveCognitive deficits in schizophrenia play a crucial role in its clinical manifestation and seem to be related to changes in the cholinergic system, specifically the action of acetylcholinesterase (AChE). Considering this context, the aim of this study was to evaluate the chronic effects of ketamine in the activity of AChE, as well as in behavioural parameters involving learning and memory.MethodsThe ketamine was administered for 7 days. A duration of 24 h after the last injection, the animals were submitted to behavioural tests. The activity of AChE in prefrontal cortex, hippocampus and striatum was measured at different times after the last injection (1, 3, 6 and 24 h).ResultsThe results indicate that ketamine did not affect locomotor activity and stereotypical movements. However, a cognitive deficit was observed in these animals by examining their behaviour in inhibitory avoidance. In addition, an increase in AChE activity was observed in all structures analysed 1, 3 and 6 h after the last injection. Differently, serum activity of AChE was similar between groups.ConclusionChronic administration of ketamine in an animal model of schizophrenia generates increased AChE levels in different brain tissues of rats that lead to cognitive deficits. Therefore, further studies are needed to elucidate the complex mechanisms associated with schizophrenia.