bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of using adipose-derived stem cells and PRP on regeneration of 40 -mm long sciatic nerve defect bridged by polyglycolic-polypropylene mesh in canine model
Authors: Khaled Mona M. , Ibrahium Asmaa M. , Abdelgalil Ahmed I. , El-Saied Mohamed A. , Yassin Aya M. , Abouquerin Nagy , Rizk Hamdy , El-Bably Samah H. 
Published: July 2024

Abstract
Background
Sciatic nerve repair becomes a focus of research in neurological aspect to restore the normal physical ability of the animal to stand and walk. Tissue engineered nerve grafts (TENGs) provide a promising alternative therapy for regeneration of large gap defects. The present study investigates the regenerative capacity of PRP, ADSCs, and PRP mixed ADSCs on a long sciatic nerve defect (40-mm) bridged by a polyglycolic polypropylene (PGA-PRL) mesh which acts as a neural scaffold.

Materials and methods
The study was conducted on 12 adult male mongrel dogs that were randomly divided into 4 groups: Group I (scaffold group); where the sciatic defect was bridged by a (PGA-PRL) mesh only while the mesh was injected with ADSCs in Group II (ADSCs group), PRP in Group III (PRP group). Mixture of PRP and ADSCs was allocated in Group IV (PRP + ADSCs group). Monthly, all animals were monitored for improvement in their gait and a numerical lameness score was recorded for all groups. 6 months-post surgery, the structural and functional recovery of sciatic nerve was evaluated electrophysiologically, and on the level of gene expression, and both sciatic nerve and the gastrocnemius muscle were evaluated morphometrically, histopathologically.

Results
Numerical lameness score showed improvement in the motor activities of both Group II and Group III followed by Group IV and the scaffold group showed mild improvement even after 6 months. Histopathologically, all treated groups showed axonal sprouting and numerous regenerated fascicles with obvious angiogenesis in proximal cut, and distal portion where Group IV exhibited a significant remyelination with the MCOOL technique. The regenerative ratio of gastrocnemius muscle was 23.81%, 56.68%, 52.06% and 40.69% for Group I, II, III and IV; respectively. The expression of NGF showed significant up regulation in the proximal portion for both Group III and Group IV (P ≤ 0.0001) while Group II showed no significant difference. PDGF-A, and VEGF expressions were up-regulated in Group II, III, and IV whereas Group I showed significant down-regulation for NGF, PDGF-A, and VEGF (P ≤ 0.0001).

Conclusion
ADSCs have a great role in restoring the damaged nerve fibers by secreting several types of growth factors like NGF that have a proliferative effect on Schwann cells and their migration. In addition, PRP therapy potentiates the effect of ADSCs by synthesis another growth factors such as PDGF-A, VEGF, NGF for better healing of large sciatic gap defects.
Human umbilical cord mesenchymal stem cell-based gene therapy for hemophilia B using scAAV-DJ/8-LP1-hFIXco transduction
Authors: Bu Zibin , Lou Jintu , Xu Weiqun , Zhang Lingyan , Tang Yongmin 
Published: July 2024

Abstract
Background
Hemophilia B is an X-linked bleeding disorder caused by a mutation in the gene responsible for encoding coagulation factor IX (FIX). Gene therapy offers promising potential for curing this disease. However, the current method of relatively high dosage of virus injection carries inherent risks. The purpose of this study was to introduce a novel scAAV-DJ/8-LP1-hFIXco vector transduced human umbilical cord blood derived mesenchymal stem cells (HUCMSCs) as an alternative cell-based gene therapy to conventional gene therapy for Hemophilia B.

Methods
The LP1-hFIXco gene structure was designed by us through searching the literature from NCBI and the scAAV-DJ/8-LP1-hFIXco vector was constructed by a commercial company. The HUCMSCs were cultivated in routine approach and transduced with scAAV-DJ/8-LP1-hFIXco vector. The human FIX activation system was employed for detection of hFIXco activity. The RNA and protein expression levels of the hFIXco were evaluated using PCR and western blot techniques. In animal studies, both NSG and F9-KO mice were used for the experiment, in which clotting time was utilized as a parameter for bleeding assessment. The immunohistochemical analysis was used to assess the distribution of HUCMSCs in mouse tissue sections. The safety for tumorigenicity of this cell-based gene therapy was evaluated by pathological observation after hematoxylin-eosin staining.

Results
The transduction of HUCMSCs with the scAAV-DJ/8-LP1-hFIXco vector results in consistent and sustainable secretion of human FIXco during 5 months period both in vitro and in mouse model. The secretion level (hFIXco activity: 97.1 ± 2.3% at day 7 to 48.8 ± 4.5% at 5 months) was comparable to that observed following intravenous injection with a high dose of the viral vector (hFIXco activity: 95.2 ± 2.2% to 40.8 ± 4.3%). After a 5-month observation period, no clonal expansions of the transduced cells in tissues were observed in any of the mice studied.

Conclusions
We have discovered a novel and safer HUCMSCs mediated approach potentially effective for gene therapy in hemophilia B.
Protein-free media for cardiac differentiation of hPSCs in 2000 mL suspension culture
Authors: Kriedemann Nils , Manstein Felix , Hernandez-Bautista Carlos A. , Ullmann Kevin , Triebert Wiebke , Franke Annika , Mertens Mira , Stein Inês Carvalheira Arnaut Pombeiro , Leffler Andreas , Witte Merlin , Askurava Tamari , Fricke Veronika , Gruh Ina , Piep Birgit , Kowalski Kathrin , Kraft Theresia , Zweigerdt Robert 
Published: July 2024

Abstract
Background
Commonly used media for the differentiation of human pluripotent stem cells into cardiomyocytes (hPSC-CMs) contain high concentrations of proteins, in particular albumin, which is prone to quality variations and presents a substantial cost factor, hampering the clinical translation of in vitro-generated cardiomyocytes for heart repair. To overcome these limitations, we have developed chemically defined, entirely protein-free media based on RPMI, supplemented with L-ascorbic acid 2-phosphate (AA-2P) and either the non-ionic surfactant Pluronic F-68 or a specific polyvinyl alcohol (PVA).

Methods and Results
Both media compositions enable the efficient, directed differentiation of embryonic and induced hPSCs, matching the cell yields and cardiomyocyte purity ranging from 85 to 99% achieved with the widely used protein-based CDM3 medium. The protein-free differentiation approach was readily up-scaled to a 2000 mL process scale in a fully controlled stirred tank bioreactor in suspension culture, producing > 1.3 × 109 cardiomyocytes in a single process run. Transcriptome analysis, flow cytometry, electrophysiology, and contractile force measurements revealed that the mass-produced cardiomyocytes differentiated in protein-free medium exhibit the expected ventricular-like properties equivalent to the well-established characteristics of CDM3-control cells.

Conclusions
This study promotes the robustness and upscaling of the cardiomyogenic differentiation process, substantially reduces media costs, and provides an important step toward the clinical translation of hPSC-CMs for heart regeneration.
Healthcare workers’ knowledge, attitude and practices on infection prevention and control in the context of the COVID-19 pandemic at the Faranah regional hospital and associated healthcare centers, Guinea
Authors: Landsmann Lena , Borodova Anna , Rocha Carlos , Diallo Aziz Amadou , Diallo Kamis Mamadou , Borchert Matthias , Arvand Mardjan , Diallo Mamadou , Wood Rebekah R. , Müller Sophie A. 
Published: July 2024

Abstract
Background
In response to the COVID-19 pandemic, WHO launched a strategic preparedness and response plan, outlining public health measures to support countries worldwide. Healthcare workers have an increased risk of becoming infected and their behaviour regarding infection prevention and control (IPC) influences infection dynamics. IPC strategies are important across the globe, but even more in low-resource settings where capacities for testing and treatment are limited. Our study aimed to assess and implement COVID-19 pandemic preparedness and response measures in Faranah, Guinea, primarily focusing on healthcare workers’ IPC knowledge, attitude and practice (KAP).

Methods
The study was conducted between April 2020 and April 2021 assessing IPC pandemic preparedness and response measures such as healthcare workers’ KAP, alcohol-based handrub (ABHR) consumption and COVID-19 triaging in the Faranah Regional Hospital and two associated healthcare centres. The assessment was accompanied by IPC training and visual workplace reminders and done in pre- and post- phases to evaluate possible impact of these IPC activities.

Results
The overall knowledge score in the Faranah Regional Hospital was 32.0 out of 44 at baseline, and did not change in the first, but increased significantly by 3.0 points in the second follow-up. The healthcare workers felt closer proximity to SARS-CoV-2 overtime in addition to higher stress levels in all study sites. There was significant improvement across the observed triaging practices. Hand hygiene compliance showed a significant increase across study sites leading to 80% in Faranah Regional Hospital and 63% in healthcare centers. The average consumption of ABHR per consultation was 3.29 mL with a peak in February 2020 of 23 mL.

Conclusion
Despite increased stress levels among HCWs, the ongoing IPC partnership well prepared the FRH in terms of triaging processes with a stronger impact on IPC practice than on theoretical knowledge. Throughout the pandemic, global shortages and surges in consumption did not affect the continuous ABHR provision of the FRH. This highlights local ABHR production as a key pandemic preparedness strategy.
Eating disorders, body image dissatisfaction and their association with gluten-free diet adherence among patients with celiac disease
Authors: Rabiee Reyhaneh , Mahdavi Reza , Shirmohammadi Masood , Nikniaz Zeinab 
Published: July 2024

Abstract
Background
Considering the higher prevalence of psychological problems in patients with Celiac disease (CD), the current study aims to assess the prevalence of eating disorders (EDs) and body image disturbance in patients with CD and examine the possible correlation between EDs, body image dissatisfaction and distortion, and gluten-free diet (GFD) adherence in these patients.

Methods
In this cross-sectional study, 217 patients with CD (18–55 years old) were recruited randomly from the CD registry database. EDs and body image issues were assessed using the 26-item Eating Attitude Test (EAT-26) and Stunkard Figure Rating Scale (FRS), respectively. Adherence to GFD was evaluated by the Celiac Dietary Adherence Test (CDAT) questionnaire.

Results
The prevalence of EDs was 43.5%. Furthermore, the prevalence of body dissatisfaction and distortion was 65.9% and 41.1%, respectively. The logistic regression demonstrated a significant negative association between adherence to the GFD and EDs (OR = 2.09, 95% CI: 1.11–3.91, P = 0.022). However, there was no significant association between following GFD and body image dissatisfaction (OR = 1.70, CI: 0.92–3.17, P = 0.090), and distortion (OR = 0.65, CI: 0.36–1.18, P = 0.163).

Conclusion
Considering the high prevalence of EDs in patients with CD and owing to the inverse association between EDs and GFD adherence, nutritionists should consider the psychological barriers in adhering to a GFD when consulting patients with CD.
Perceptions of artificial intelligence system's aptitude to judge morality and competence amidst the rise of Chatbots
Authors: Oliveira Manuel , Brands Justus , Mashudi Judith , Liefooghe Baptist , Hortensius Ruud 
Published: July 2024

AbstractThis paper examines how humans judge the capabilities of artificial intelligence (AI) to evaluate human attributes, specifically focusing on two key dimensions of human social evaluation: morality and competence. Furthermore, it investigates the impact of exposure to advanced Large Language Models on these perceptions. In three studies (combined N = 200), we tested the hypothesis that people will find it less plausible that AI is capable of judging the morality conveyed by a behavior compared to judging its competence. Participants estimated the plausibility of AI origin for a set of written impressions of positive and negative behaviors related to morality and competence. Studies 1 and 3 supported our hypothesis that people would be more inclined to attribute AI origin to competence-related impressions compared to morality-related ones. In Study 2, we found this effect only for impressions of positive behaviors. Additional exploratory analyses clarified that the differentiation between the AI origin of competence and morality judgments persisted throughout the first half year after the public launch of popular AI chatbot (i.e., ChatGPT) and could not be explained by participants' general attitudes toward AI, or the actual source of the impressions (i.e., AI or human). These findings suggest an enduring belief that AI is less adept at assessing the morality compared to the competence of human behavior, even as AI capabilities continued to advance.
Energy management controllers: strategies, coordination, and applications
Authors: Bakare Mutiu Shola , Abdulkarim Abubakar , Shuaibu Aliyu Nuhu , Muhamad Mundu Mustafa 
Published: July 2024

AbstractEnergy management controllers (EMCs) are pivotal for optimizing energy consumption and ensuring operational efficiency across diverse systems. This review paper delves into the various control strategies utilized by energy management controllers and explores their coordination mechanisms. Additionally, it examines the architectures of energy management controllers and their real-world implementations. The paper surveys a spectrum of EMCs, including conventional-based, rule-based, optimization-based, hybrid methods, and commercial software-based approaches, highlighting their respective advantages and drawbacks. It investigates how these controllers are coordinated within complex energy systems to achieve optimal performance and adaptability. Furthermore, the review outlines different architectures of energy management controllers, ranging from centralized to decentralized designs, discussing their suitability for various applications and their impact on system performance. Real-world applications of energy management controllers in sectors such as smart grids, buildings, industrial processes, and transportation systems are examined. Case studies and examples demonstrate the efficacy of different control strategies and architectures in addressing specific energy management challenges and achieving desired outcomes. Overall, this review provides valuable insights into the current landscape of energy management controller design and implementation, offering direction for future research and development in the pursuit of energy optimization and sustainability.
Optimization configuration strategy for regional energy systems based on multiple uncertainties and demand response
Authors: Zhao Rui , Chen Xinghua 
Published: July 2024

AbstractWith the opening of the power market and the development of the energy Internet, the optimal allocation of regional energy systems has become the key to achieving energy efficiency and economic balance. The article studies how to achieve this balance by optimizing the allocation of regional energy systems under the influence of price fluctuations and load demand uncertainty in the electricity market. This study introduces a real-time electricity price adjustment mechanism to stimulate user participation in energy adjustments and improve energy utilization efficiency. By constructing an optimization model based on multiple uncertainties and comprehensive demand response, uncertainty factors such as energy market price fluctuations and climate change were considered, and user demand response was integrated. The research results indicate that electricity price fluctuations have a significant impact on system operation, while CSP power plant thermal storage fluctuations have a relatively small impact. After the introduction of demand response, the electricity load can be reduced to zero during specific periods, and the adjustment of electricity prices stimulates user participation and improves the consumption rate of renewable energy. The total revenue of the system increased by 54.147 million yuan, demonstrating the potential of optimized configuration in reducing costs and improving efficiency. This study provides important references for building more efficient and sustainable energy systems.
Exploring flexural performance and abrasion resistance in recycled brick powder-based engineered geopolymer composites
Authors: Ahmed Junaid K. , Atmaca Nihat , Khoshnaw Ganjeena J. 
Published: July 2024

Abstract
Background
Due to growing global concerns regarding the management of construction waste, this study investigates the feasibility of creating engineered geopolymer composites by replacing traditional industrial by-products (slag) with construction waste, specifically recycled brick waste powder.

Results
Polyvinyl alcohol fibers were incorporated into the engineered geopolymer composite mixtures. The substitution of slag with recycled brick waste powder was carried out at varying percentages: 0, 20, 40, 60, 80, and 100%, resulting in six different engineered geopolymer composite mixtures. The study evaluated the flexural strength, sorptivity, water absorption, and abrasion resistance of the engineered geopolymer composites, and also, microstructural characterization was conducted using scanning electron microscopy. The findings demonstrated that incorporating recycled brick waste powder into the engineered geopolymer composite mixes resulted in a decrease in flexural strength by 35.59% and a notable increase in midspan deflection by 339% when slag was replaced. Concurrently, there was a significant rise in water absorption and sorptivity by approximately 304 and 214%, respectively, when slag was entirely substituted with recycled brick waste powder. Conversely, abrasion resistance decreased, with the inclusion of recycled brick waste powder resulting in an 84% increase in volume change. The scanning electron microscopy (SEM) analysis showed active geopolymerization of recycled brick waste powder within the engineered geopolymer composite mixtures.

Conclusions
The results of this investigation demonstrate that it is feasible to produce engineered geopolymer composites using recycled brick waste powder instead of slag. The greater ductility and increased midspan deflection point to areas that require further optimization, even in spite of the observed decreases in flexural strength and abrasion resistance. The SEM examination reveals an active geopolymerization, highlighting the potential of recycled brick waste powder to produce environmentally friendly and sustainable construction materials. These results offer a good starting point for further studies that try to maximize the durability and performance of these composites.
Adenocarcinoma of unknown primary with TP53 gene polymorphism: a rare case report with literature review
Authors: Chaudhary Raushan Kumar , Mateti Uday Venkat , Shetty Jayaprakash , Patil Prakash , Sangamesh Vinay C. , Shetty Vijith Vittal 
Published: July 2024

Abstract
Background
Cancer of unknown primary (CUP) is an orphan disease generally presented by undifferentiated and aggressive morphological phenotype. The treatment of CUP is solely dependent upon the origin of cancer. Despite extensive diagnostic testing, in most of the cases the primary site remains unidentifiable.

Case presentation
This case demonstrates a 75-year-old male patient, who initially presented with the complaints of swelling over right side of the neck since 2 months. A cervical lymph node biopsy was taken for immunohistochemistry, which revealed cytokeratin (CK) and CK7 markers to be positive. Computerized tomography (CT) of Thorax showed subcentimetric subpleural nodules in bilateral lungs fields, predominantly in lower lobes (metastatic in nature). A subsequent pulmoCORE 12 gene panel test was recommended, and patient was discharged with tablet gefitinib 250mg and capsule containing vitamins plus minerals. After one month, patient revisited with the pulmoCORE 12 gene test report which revealed polymorphism in TP53. A pathogenic variant of tumor protein p53 (TP53), i.e., p.Glu198Ter (amino acid alteration) and c.592G > T (coding) variant, was detected, which has 17.2% variant allele frequency. There are no treatment guidelines for TP53 mutation; therefore, the patient was treated with injection paclitaxel 70mg and carboplatin 100mg for 12 cycles along with palliative radiotherapy of 20 Gy for 5 fractions. The overall prognosis of patient was found to be favorable.

Conclusions
There is a need for development of comprehensive guidelines and new molecularly targeted therapies for treatment of CUP which can be tailored for each patient and achieve precise therapeutic outcome.
Discordance Between the Initial Diagnosis of Sarcomas and Subsequent Histopathological Revision and Molecular Analyses in a Sarcoma Reference Center in Brazil
Authors: Lopes Carlos Diego H. , Queiroz Marcello M. , Sampaio Luana A.F. , Perina André , Akaishi Eduardo , Teixeira Frederico , Ferreira Fábio de O. , Hanna Samir A. , da Silva João Luís F. , De Lima Luiz Guilherme C.A. , de Oliveira Claudia Regina G.C.M. , Munhoz Rodrigo R. 
Published: July 2024

PURPOSE To investigate the discordance in sarcoma diagnoses between nonspecialized institutions following revision by dedicated sarcoma pathologists at a reference center in Brazil and the relevance of molecular pathology in this context. METHODS We conducted a retrospective analysis of sarcoma samples initially analyzed at outside laboratories and subsequently reviewed by two specialized pathologists between January 2014 and December 2020. After obtaining demographic and tumor characteristics, pathology results were matched and classified as complete discordance (CD; benign v malignant, sarcoma v other malignancies), partial concordance (similar diagnosis of connective tumor, but different grade/histological subtype/differentiation), and complete concordance (CC). The concordance for histology or grade, and the role of molecular assessments supporting the diagnosis were also independently determined. Statistical analyses were conducted through the kappa coefficient of agreement and adherence by χ2 test, χ2 test by Person, and Fisher exact test. RESULTS In total, 197 cases were included, with samples obtained predominately from male patients (57.9%) and localized/primary tumors (86.8%). Following revision, the most frequent final diagnoses were undifferentiated pleomorphic sarcoma (17.8%), well-differentiated/dedifferentiated liposarcoma (8.6%), and leiomyosarcoma (7.6%). CD was found in 13.2%, partial discordance in 45.2%, and CC in 41.6% of reviews ( P < .001). We found a concordance for histology or grade of 53.5% ( P < .001) and 51.8% ( P < .001), respectively. Molecular assessments, comprising next-generation sequencing panels (79.5%) and fluorescent in situ hybridization (20.5%), were performed in 44 (22.3%) cases, with findings classified as of diagnostic relevance in 31.8%. CONCLUSION In nearly 60% of the cases, the initial sarcoma diagnosis was modified when revised by a reference center and dedicated pathologists, assisted by molecular pathology techniques. These results justify the assembly of referral networks in countries with limited health care resources.