bluuebunny/crossref_metadata_2025_split huggingface dataset:
Communicating science and technology while engaging the public at the Illinois Basin – Decatur Project
Authors: Greenberg Sallie E. , Gauvreau Lori M. 
Published: September 2014

AbstractThe Midwest Geological Sequestration Consortium communication initiative at the Illinois Basin – Decatur Project (IBDP) draws on multiple carbon capture and storage (CCS) best practices, guidelines, and international project experiences to be both proactive and responsive toward the engagement of multiple stakeholders. The IBDP communications strategy was developed early in the project to actively reduce associated risks by creating and implementing a communication plan, training communicators, and providing a structure for the communications team. Formalized approaches to knowledge sharing and capacity building have generated additional opportunities to further outreach and impact from IBDP experiences. The initial challenge for the communications team was to provide easy‐to‐understand, scientifically accurate, and consistent information for stakeholders to carry throughout the project. Consistent, factual information was developed and incorporated into project planning, and provides the basis of public communications through the alignment of goals across communications, risk mitigation, and project management. The refinement of the communication strategy and plan is ongoing due to the changing communication needs that are encountered throughout the lifetime of the project.
The structure of the amygdala associates with human sexual permissiveness: Evidence from voxel‐based morphometry
Authors: Takeuchi Hikaru , Taki Yasuyuki , Nouchi Rui , Hashizume Hiroshi , Sekiguchi Atsushi , Kotozaki Yuka , Nakagawa Seishu , Miyauchi Carlos Makoto , Sassa Yuko , Kawashima Ryuta 
Published: September 2014

AbstractSexual behavior is a critical function of human procreation. Despite previous studies that investigated the neural mechanisms of basic human physiological sexual functions, the neural mechanisms that underlie individual differences in human sexual permissiveness remain unknown. We used voxel‐based morphometry and a questionnaire (scale for sexual attitudes) to measure sexual permissiveness to investigate the gray matter and white matter structural correlates of sexual permissiveness. Sexual permissiveness was negatively correlated with regional gray matter density of the structures involving the right amygdala and surrounding areas, and positively correlated with regional white matter density of the white matter area that spread around the left amygdala to the hypothalamus area. There were no gender‐specific relationships in the neural correlates of our findings. These findings suggest that structural variations in regions that play key roles in the basic physiological aspects of human sexuality underlie individual complex sexual attitudes in social life. Hum Brain Mapp 36:440–448, 2015. © 2014 The Authors. Human Brain Mapping Published by Wiley Periodicals, Inc.
In vitromechanical fatigue behavior of poly‐ɛ‐caprolactone macroporous scaffolds for cartilage tissue engineering: Influence of pore filling by a poly(vinyl alcohol) gel
Authors: Panadero J. A. , Vikingsson L. , Gomez Ribelles J. L. , Lanceros‐Mendez S. , Sencadas V. 
Published: September 2014

AbstractPolymeric scaffolds used in regenerative therapies are implanted in the damaged tissue and submitted to repeated loading cycles. In the case of articular cartilage engineering, an implanted scaffold is typically subjected to long‐term dynamic compression. The evolution of the mechanical properties of the scaffold during bioresorption has been deeply studied in the past, but the possibility of failure due to mechanical fatigue has not been properly addressed. Nevertheless, the macroporous scaffold is susceptible to failure after repeated loading‐unloading cycles. In this work fatigue studies of polycaprolactone scaffolds were carried by subjecting the scaffold to repeated compression cycles in conditions simulating the scaffold implanted in the articular cartilage. The behavior of the polycaprolactone sponge with the pores filled with a poly(vinyl alcohol) gel simulating the new formed tissue within the pores was compared with that of the material immersed in water. Results were analyzed with Morrow's criteria for failure and accurate fittings are obtained just up to 200 loading cycles. It is also shown that the presence of poly(vinyl alcohol) increases the elastic modulus of the scaffolds, the effect being more pronounced with increasing the number of freeze/thawing cycles. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1037–1043, 2015.
Incorporation of copper into chitosan scaffolds promotes bone regeneration in rat calvarial defects
Authors: D'Mello Sheetal , Elangovan Satheesh , Hong Liu , Ross Ryan D. , Sumner D. Rick , Salem Aliasger K. 
Published: September 2014

AbstractThe objective of this study was to investigate the effects of a copper loaded chitosan scaffold on bone regeneration in critical‐sized calvarial defects in rats. Chitosan scaffolds and copper‐chitosan scaffolds were fabricated and characterized by scanning electron microscopy (SEM). Chitosan and copper‐chitosan scaffolds were implanted into 5 mm diameter critical‐sized calvarial defects in Fisher 344 male rats. Empty defects (no scaffolds) were included as a control. After 4 weeks, the rats were sacrificed for microcomputed tomography (micro‐CT) and histological analysis of new bone tissue development. Microscopy images revealed the uniformly porous structure of chitosan and copper‐chitosan scaffolds. Significant bone regeneration was noted in the defects treated with copper‐chitosan scaffolds when evaluated using micro‐CT and histological analysis, when compared with other groups tested. On analysis of the micro‐CT scans, an eleven‐fold and a two‐fold increase in the new bone volume/total volume (BV/TV) % was found in defects treated with the copper‐chitosan scaffolds, when compared to empty defects and chitosan scaffolds, respectively. This study demonstrated the suitability of copper‐crosslinked chitosan scaffolds for bone tissue engineering and provides the first evidence that inclusion of copper ions in scaffolds can enhance tissue regeneration. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1044–1049, 2015.
Fatty acid composition and δ13C of bulk and individual fatty acids as marker for authenticating Italian PDO/PGI extra virgin olive oils by means of isotopic ratio mass spectrometry
Authors: Faberi Angelo , Marianella Rosa Maria , Fuselli Fabio , La Mantia Alessandro , Ciardiello Felice , Montesano Camilla , Mascini Marcello , Sergi Manuel , Compagnone Dario 
Published: September 2014

European Regulation (EEC) 2568/91 has been setting the minimum requirements in order to allow labeling of oil as extra virgin. These general requirements, are based on physical–chemical and organoleptic parameters directly linked to the freshness and quality of the product.Isotope ratio mass spectrometry (IRMS) was demonstrated to be a useful tool for the discrimination of the origin of unknown samples, because the obtained data are practically independent of the cultivar employed and the production technique.In this work, the evaluation of the composition of fatty acid methyl esters (FAME) alongside with the determination of stable isotope ratio of C in bulk oils and in main FAME constituents have been investigated as a tool to improve geographical discrimination of Italian Protected Designation of Origin/Protected Geographical Indication (PDO/PGI) samples.For this purpose, authentic PDO/PGI extra virgin olive oils were sampled at oil mills and grouped into different sets according to their areas of provenience.The use of principal component analysis and partial least squares discriminant analysis multivariate analysis techniques demonstrated that discrimination of olive oil samples can be done using geographical and pedoclimatic parameters predominantly by using δ13C results of bulk and individual fatty acids. Results showed that δ13C values are a more reliable marker of origin with respect to fatty acid composition. Copyright © 2014 John Wiley & Sons, Ltd.
Stable isotope ratios of H, C, N and O in Italian citrus juices
Authors: Bontempo L. , Caruso R. , Fiorillo M. , Gambino G. L. , Perini M. , Simoni M. , Traulo P. , Wehrens R. , Gagliano G. , Camin F. 
Published: September 2014

Stable isotope ratios (SIRs) of C, N, H and O have been exensively used in fruit juices quality control (ENV and AOAC methods) to detect added sugar and the watering down of concentrated juice, practices prohibited by European legislation (EU Directive 2012/12). The European Fruit Juice Association (AIJN) set some reference guidelines in order to allow the judging of the genuiness of a juice. Moreover, various studies have been carried out to determine the natural variability of SIRs in fruit juices, but none of these has investigated SIRs extensively in authentic citrus juices from Italy. In this work, about 500 citrus juice samples were officially collected in Italy by the Italian Ministry of Agricultural and Forestry Policies from 1998 onwards. (D/H)I and (D/H)II in ethanol and δ13Cethanol, δ13Cpulp, δ13Csugars, δ18Ovegetalwater, δ15Npulp, and δ18Opulp were determined using Site‐Specific Natural Isotope Fractionation‐Nuclear Magnetic Resonance and Isotope Ratio Mass Spectrometry, respectively.The characteristic ranges of variability in SIRs in genuine Italian citrus juice samples are here presented as well as their relationships and compliance with the limits indicated by the AIJN and others proposed in the literature. In particular, the Italian range of values was found to be not completely in agreement with AIJN guidelines, with the risk that genuine juices could be judged as not genuine.Variety seems not to influence SIRs, whereas harvest year and region of origin have some influence on the different ratios, although their data distribution shows overlapping when principal component analysis is applied. Copyright © 2014 John Wiley & Sons, Ltd.
In‐office balloon catheter dilation: Analysis of 628 patients from an administrative claims database
Authors: Sillers Michael J. , Lay Kristopher F. , Holy Chantal E. 
Published: September 2014

Objectives/HypothesisBalloon catheter dilation (BCD) of the paranasal sinuses is increasingly described as a standalone procedure in the office for minimally invasive treatment of patients with chronic rhinosinusitis (CRS). This study evaluates CRS‐related healthcare utilization before and after in‐office treatment as performed by otolaryngologists within the United States in an all‐comer population.Study DesignRetrospective claims database analysis.MethodsThe MarketScan database was queried for patients undergoing BCD (Current Procedural Terminology [CPT] 31295–31297) for CRS (International Classification of Diseases Ninth Edition [ICD‐9] 473.x) since 2011 in the office (place of service: 11). Exclusion criteria included <1 year preoperative enrollment, <6 months postoperative history, and concurrent (±30 days) endoscopic sinus surgery (CPT 31254–31288). A cohort of 628 patients was identified. Analyses included preoperative comorbidities and CRS‐related healthcare use.ResultsPreoperative comorbidities included asthma (29.0%), polyps (11.5%), and aspirin sensitivity (9.9%). An average of 3.0 sinuses (95% confidence interval: 2.91–3.17) were dilated at index. Maxillary, frontal, and sphenoid dilations were performed in 88.9%, 74.5%, and 33.8% of cases, respectively. There were two orbital complications and seven cases of hemorrhage potentially associated with the procedure, but no postoperative cerebrospinal fluid leaks. No blood transfusion, lateral canthotomy, or canthoplasty were reported. CRS‐related postoperative healthcare use declined rapidly from the immediate preoperative state. Twenty‐one patients underwent revisions, with one patient undergoing two surgeries for a total of 22 reoperations (3.5%).ConclusionsIn‐office BCD is associated with low risks of complications and revisions. Whereas not significant for patients with polyps, decreases in healthcare utilization pre‐ to postsurgery were significant for patients without polyps.Level of EvidenceNA Laryngoscope, 125:42–48, 2015
Computational homogenization of soft matter friction: Isogeometric framework and elastic boundary layers
Authors: Temizer İ. 
Published: September 2014

SUMMARYA computational contact homogenization framework is established for the modeling and simulation of soft matter friction. The main challenges toward the realization of the framework are (1) the establishment of a frictional contact algorithm that displays an optimal combination of accuracy, efficiency, and robustness and plays a central role in (2) the construction of a micromechanical contact test within which samples of arbitrary size may be embedded and which is not restricted to a single deformable body. The former challenge is addressed through the extension of mixed variational formulations of contact mechanics to a mortar‐based isogeometric setting where the augmented Lagrangian approach serves as the constraint enforcement method. The latter challenge is addressed through the concept of periodic embedding, with which a periodically replicated ‐continuous interface topography is realized across which not only pending but also ensuing contact among simulation cells will be automatically captured. Two‐dimensional and three‐dimensional investigations with unilateral/bilateral periodic/random roughness on two elastic micromechanical samples demonstrate the overall framework and the nature of the macroscopic frictional response. Copyright © 2014 John Wiley & Sons, Ltd.
Targeted genomic analysis of Müllerian adenosarcoma
Authors: Howitt Brooke E , Sholl Lynette M , Dal Cin P , Jia Yonghui , Yuan Liping , MacConaill Laura , Lindeman Neal , Kuo Frank , Garcia Elizabeth , Nucci Marisa R , Quade Bradley J 
Published: September 2014

AbstractMüllerian adenosarcoma (MA) is a rare mixed mesenchymal tumour of the female genital tract, composed of malignant stroma and benign‐appearing epithelium. Sarcomatous overgrowth (SO) is the only established histological variable associated with higher stage and shorter survival. Specific molecular or immunohistochemistry (IHC) tools for the diagnosis of MA are lacking. Our goal was to study genomic mutations and copy number variations (CNVs) in MA to understand better its pathobiology, and develop specific diagnostic and prognostic tools. DNA was extracted from 20 samples of MA from 18 subjects (12 without SO and 6 with SO), including two in which areas of both typical MA histology and SO were independently tested. Samples were analysed using a targeted next‐generation sequencing assay interrogating exonic sequences of 275 cancer genes for mutations and CNVs as well as 91 introns across 30 genes for cancer‐associated rearrangements. The mean number of mutations in MA with SO (mean 9.7; range 3–14) did not differ significantly from that in MA without SO (mean 9.6; range 5–16). MA with SO had significantly higher mean numbers of gene‐level CNVs (24.6) compared to MA without SO (5; p = 0.0002). The most frequent amplification involved MDM2 and CDK4 (5/18; 28%), accompanied by focal CDK4 and MDM2 and diffuse HMGA2 expression using immunohistochemistry. MYBL1 amplification was seen in 4/18 (22%), predominantly in SO. Alterations in PIK3CA/AKT/PTEN pathway members were seen in 13/18 (72%). Notably, TP53 mutations were uncommon, present in only two cases with SO. Three out of 18 (17%) had mutations in ATRX, all associated with SO. No chromosomal rearrangements were identified. We have identified a number of recurrent genomic alterations in MA, including some associated with SO. Although further investigation of these findings is needed, confirmation of one or more may lead to new mechanistic insights and novel markers for this often difficult‐to‐diagnose tumour. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd
CXCR4 expression enhances diffuse large B cell lymphoma dissemination and decreases patient survival
Authors: Moreno María José , Bosch Rosa , Dieguez‐Gonzalez Rebeca , Novelli Silvana , Mozos Ana , Gallardo Alberto , Pavón Miguel Ángel , Céspedes María Virtudes , Grañena Albert , Alcoceba Miguel , Blanco Oscar , Gonzalez‐Díaz Marcos , Sierra Jorge , Mangues Ramon , Casanova Isolda 
Published: September 2014

AbstractThe chemokine receptor CXCR4 has been implicated in the migration and trafficking of malignant B cells in several haematological malignancies. Over‐expression of CXCR4 has been identified in haematological tumours, but data concerning the role of this receptor in diffuse large B cell lymphoma (DLBCL) are lacking. CXCR4 is a marker of poor prognosis in various neoplasms, correlating with metastatic disease and decreased survival of patients. We studied CXCR4 involvement in cell migration in vitro and dissemination in vivo. We also evaluated the prognostic significance of CXCR4 in 94 biopsies of DLBCL patients. We observed that the level of expression of CXCR4 in DLBCL cell lines correlated positively with in vitro migration. Expression of the receptor was also associated with increased engraftment and dissemination, and decreased survival time in NOD/SCID mice. Furthermore, administration of a specific CXCR4 antagonist, AMD3100, decreased dissemination of DLBCL cells in a xenograft mouse model. In addition, we found that CXCR4 expression is an independent prognostic factor for shorter overall survival and progression‐free survival in DLBCL patients. These results show that CXCR4 mediates dissemination of DLBCL cells and define for the first time its value as an independent prognostic marker in DLBCL patients. Copyright © 2014 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd
A new approach to blending starch with natural rubber
Authors: Trovatti Eliane , Carvalho Antonio José Felix , Gandini Alessandro 
Published: September 2014

AbstractThermoplastic starch (TPS) is the most widely used renewable and biodegradable polymer in applications such as packaging and disposable materials due to its properties, abundance and low cost. Research aimed at widening its range of applications includes the improvement of its mechanical properties by mixing it with other polymers. In this study, TPS was blended with oxidized natural rubber (ONR) in order to enhance its plasticity through higher interface compatibility. The oxidation of natural rubber was carried out to promote chemical interactions between the hydroxyl groups of ONR with those of TPS through hydrogen bonding. The blends were prepared by extrusion and characterized using dynamic mechanical analysis, tensile tests, contact angle measurements, water absorption and scanning electron microscopy. The results showed that these new materials displayed the expected improvements, as well as better water resistance and decreased brittleness. © 2014 Society of Chemical Industry
A comprehensive database of verified experimental data on protein folding kinetics
Authors: Wagaman Amy S. , Coburn Aaron , Brand‐Thomas Itai , Dash Barnali , Jaswal Sheila S. 
Published: September 2014

AbstractInsights into protein folding rely increasingly on the synergy between experimental and theoretical approaches. Developing successful computational models requires access to experimental data of sufficient quantity and high quality. We compiled folding rate constants for what initially appeared to be 184 proteins from 15 published collections/web databases. To generate the highest confidence in the dataset, we verified the reported lnkf value and exact experimental construct and conditions from the original experimental report(s). The resulting comprehensive database of 126 verified entries, ACPro, will serve as a freely accessible resource (https://www.ats.amherst.edu/protein/) for the protein folding community to enable confident testing of predictive models. In addition, we provide a streamlined submission form for researchers to add new folding kinetics results, requiring specification of all the relevant experimental information according to the standards proposed in 2005 by the protein folding consortium organized by Plaxco. As the number and diversity of proteins whose folding kinetics are studied expands, our curated database will enable efficient and confident incorporation of new experimental results into a standardized collection. This database will support a more robust symbiosis between experiment and theory, leading ultimately to more rapid and accurate insights into protein folding, stability, and dynamics.
Diversity and Community Structure of Fishes in the Regulated Versus Unregulated Tributaries of the Mekong River
Authors: Phomikong P. , Fukushima M. , Sricharoendham B. , Nohara S. , Jutagate T. 
Published: September 2014

AbstractDiversity and community structure of fishes were studied in three neighbouring tributaries of the Mekong River in Thailand, namely the Mun, Songkhram and Gam Rivers. The rivers are located in the same ecoregion but have contrasting levels of both hydrological regulations and mitigation measures; the Mun River has a hydropower dam with a fish ladder and sluice gates that are opened during the wet season each year, the Gam River has several irrigation dams with a fish ladder at each dam site, and the Songkhram River has no dams along its river course. A total of 124 freshwater fish species were sampled in these rivers from August 2009 to June 2010. Overall species richness was highest in the Songkhram River (112), followed by the Mun (97) and Gam (54) Rivers. Average per site species richness was also significantly different among rivers but not among sampling months. Abundance–biomass comparison plots revealed considerably overlapping distributions of these two metrics from the dry to early rainy seasons in the Songkhram River and, to a lesser extent, in the Mun River. Fish assemblage data were classified into six clusters with similar community structure. Fish assemblages in the Gam River constituted a single cluster, while those in the other two rivers formed multiple clusters depending on the sampling season. The results of the cluster analysis are discussed in relation to the dominance of the three migration guilds (white, black, and grey fishes) of the Mekong River fishes. The effectiveness of the mitigation measures was determined to be limited in alleviating adverse impacts of dams in these tributaries of the Mekong River. Copyright © 2014 John Wiley & Sons, Ltd.