bluuebunny/crossref_metadata_2025_split huggingface dataset:
A rapid, sensitive and solvent‐less method for determination of malonaldehyde in meat by stir bar sorptive extraction coupled thermal desorption and gas chromatography/mass spectrometry with in situ derivatization
Authors: Ruan Eric Dongliang , Aalhus Jennifer , Juárez Manuel 
Published: November 2014

RATIONALEThe traditional methods for analysis of malonaldehyde (MDA), such as the thiobarbituric acid (TBA) assay, require strong acidity at high temperature for derivatization and lack specificity in analysis. Stir bar sorptive extraction (SBSE) coupled with thermal desorption‐gas chromatography/mass spectrometry (TD‐GC/MS) with in situ derivatization using pentafluorophenylhydrazine (PFPH) under mild conditions is an emerging technique for MDA analysis.METHODSMDA in meat was derivatized with PFPH at pH ~4 for 1 h at room temperature, forming a relative stable derivative of MDA‐PFPH. The derivative of MDA‐PFPH was simultaneously extracted using SBSE. Then, MDA‐PFPH was thermally released and quantitatively analyzed by GC/MS in selected ion monitoring (SIM) mode.RESULTSThe method of SBSE‐TD‐GC/MS for MDA analysis with in situ derivatization was optimized and validated with good linearity, specificity and limit of detection/quantification (LOD/LOQ). The method was successfully applied for analysis of MDA in raw and cooked meat (pork).CONCLUSIONSThe SBSE‐TD‐GC/MS method was suitable to monitor and analyze MDA in meat samples at trace levels. The simple, sensitive and solvent‐less method with moderated in situ derivatization can be applied for analysis of MDA in a wide variety of foods and biological samples. Copyright © 2014 John Wiley & Sons, Ltd.
Studies on quantitative phosphopeptide analysis by matrix‐assisted laser desorption/ionization mass spectrometry without label, chromatography or calibration curves
Authors: Ho Hsin‐Pin , Rathod Pratikkumar , Louis Marissa , Tada Christine K. , Rahaman Sherida , Mark Kevin J. , Leng Jin , Dana Dibyendu , Kumar Sanjai , Lichterfeld Mathias , Chang Emmanuel J. 
Published: November 2014

RATIONALEMatrix‐assisted laser desorption/ionization (MALDI) mass spectrometry combined with isotope labeling methods are effective for protein and peptide quantification, but limited in their multiplexing capacity, cost‐effectiveness and dynamic range. This study investigates MALDI‐MS‐based quantification of peptide phosphorylation without labeling, and aims to overcome the shot‐to‐shot variability of MALDI using a mathematical transformation and extended data acquisition times.METHODSA linear relationship between the reciprocal of phosphopeptide mole fraction and the reciprocal of phosphorylated‐to‐unphosphorylated signal ratio is derived, and evaluated experimentally using three separate phosphopeptide systems containing phosphorylated serine, threonine and tyrosine residues: mixtures of phosphopeptide and its des‐phospho‐analog with known stoichiometry measured by vacuum MALDI‐linear ion trap mass spectrometry and fit to the linear model. The model is validated for quantifying in vitro phosphorylation assays with inhibition studies on Cdk2/cyclinA.RESULTSDynamic range of picomoles to femtomoles, good accuracy (deviations of 1.5–3.0% from expected values) and reproducibility (relative standard deviation (RSD) = 4.3–6.3%) are achieved. Inhibition of cyclin‐dependent kinase phosphorylation by the classical inhibitors olomoucine and r‐roscovitine was evaluated and IC50 values found to be in agreement with reported literature values. These results, achieved with single‐point calibration, without isotope or chromatography, compare favorably to those arrived at using isotope dilution (p >0.5 for accuracy).CONCLUSIONSThe mathematical relationship derived here can be applied to a method that we term Double Reciprocal Isotope‐free Phosphopeptide Quantification (DRIP‐Q), as a strategy for quantification of in vitro phosphorylation assays, the first MALDI‐based, isotope‐ and calibration curve‐free method of its type. These results also pave the way for further systematic studies investigating the effect of peptide composition and experimental conditions on quantitative, label‐free MALDI. Copyright © 2014 John Wiley & Sons, Ltd.
Identification of xenobiotic metabolites from biological fluids using flow injection analysis high‐resolution mass spectrometry and post‐acquisition data filtering
Authors: Rathahao‐Paris Estelle , Paris Alain , Bursztyka Julian , Jaeg Jean‐Philippe , Cravedi Jean‐Pierre , Debrauwer Laurent 
Published: November 2014

RATIONALEConcern for public health entails the need to evaluate the degree of exposure of population to toxicants. To do this, robust high‐throughput approaches are required to be able to perform a large number of analyses in cohort studies. In this study, a data‐filtering procedure was applied to mass spectral data acquired by direct analysis of biological fluids leading to rapid detection of metabolites in a model xenobiotic system.METHODSFlow injection analysis (FIA) coupled to negative electrospray ionization (ESI)‐LTQ Orbitrap Fourier transform mass spectrometry was used to directly analyze urine of rats treated with vinclozolin. Tandem mass spectrometry (MS/MS) experiments were subsequently performed for confirmation of a new metabolite structure. The isotope filtering based on the difference between accurate masses of 35Cl and 37Cl was applied to the raw data for the specific detection of ions containing at least one chlorine atom.RESULTSSeven metabolites of vinclozolin were manually identified thanks to the characteristic isotope pattern of dichlorinated compounds. A new metabolite of vinclozolin was detected for the first time and identified as a sulfate conjugate. The application of an isotope‐filtering procedure allowed the selective extraction of pertinent signals from the data. The processed mass spectrum was greatly simplified, significantly facilitating the detection of the seven metabolites previously identified.CONCLUSIONSThe use of FIA‐HRMS in combination with dedicated bio‐informatics data processing is shown to be an efficient approach for the rapid detection of metabolites in biological fluids. This is a very promising high‐throughput approach for rapid characterization of the exposure status to xenobiotics. Copyright © 2014 John Wiley & Sons, Ltd.
Glycogen pathways in disease: new developments in a classical field of medical genetics
Authors: Kilimann Manfred W. , Oldfors Anders 
Published: November 2014

SummaryGlycogen is the storage form of glucose in animal cells. Its degradation can rapidly provide fuel for energy production (particularly important in muscle), or replenish blood glucose during fasting by the liver. Genetic defects of glycogen metabolism give rise to glycogen storage diseases (GSDs), manifesting histologically in abnormal quantity or quality of glycogen in the cells. GSDs can be caused by defects of proteins participating in the synthesis or degradation of glycogen itself, in the glycolytic degradation of glucose phosphates in muscle and erythrocytes, in the release of glucose from liver and kidney into the bloodstream, in the clearance of glycogen from lysosomes (all, “primary GSDs”), or in the control of these pathways (“secondary GSDs”). Most genes responsible for classical, primary GSDs have probably been identified, and future progress in understanding the biochemical and genetic defects underlying unsolved disorders presenting with glycogen storage abnormalities will perhaps be predominantly in the field of secondary GSDs.
Cognitive profile of patients with glycogen storage disease type III: a clinical description of seven cases
Authors: Michon Claire‐Cécile , Gargiulo Marcela , Hahn‐Barma Valérie , Petit François , Nadaj‐Pakleza Aleksandra , Herson Ariane , Eymard Bruno , Labrune Philippe , Laforet Pascal 
Published: November 2014

AbstractBackgroundGlycogen storage disease type III (GSDIII) is a rare autosomal recessive disorder due to glycogen debranching enzyme (GDE) deficiency. It results in a multisystemic disease with predominant hepatic and myopathic symptoms. While frequent social maladjustment has been observed in our clinical practice, cognitive and psychological disturbances have never been assessed. The aim of this pilot study was to examine and characterize the cognitive profile of patients with GSDIII.MethodsSeven patients (six women and one man, mean age: 38.7 ± 11.6 years) with GSDIII underwent a neuropsychological set of tests assessing global cognitive efficiency, executive functions, social cognition, apathy, and episodic memory.ResultsAll patients presented previous psychopathological history. We observed attention fluctuations for each patient, and impaired global cognitive efficiency with deficiencies in executive functions in 5/7 patients. Emotional skills (social cognition) were impaired in five patients. Memory was mostly preserved.ConclusionThe impairment in social cognition (recognition of emotions and ability to attribute mental states to others) and executive functions observed could be a consequence of orbito‐frontal dysfunction due to the abnormal glycogen metabolism characteristic of the underlying disease. These results are consistent with the hypothesis of a central nervous system involvement in patients with GSDIII, but need to be confirmed in future research. This could explain the social and economic difficulties, and the lack of compliance to the medical follow‐up presented by these patients. It suggests that these disturbances need to be taken into account when planning the medical management of patients with GSDIII.
Workplace care: a sensible form of support to convalescents from occupational injuries or diseases
Authors: Taddicken Markus 
Published: November 2014

In order to ensure the success of medical and occupational rehabilitation, the BGW – the occupational insurer – offers its clients to accompany them at the workplace. This aims at transferring the working methods, which the rehabilitant has acquired during rehabilitation and are therefore partly new to him, into operational practice and at verifying their fitness for practical applicability. Apart from that, this shall facilitate to identify factors jeopardizing reintegration and to beneficially influence them, if possible.Work is resumed with the approval by the client in question and their employer, accompanied by a specialized workplace attendant. Depending on the severity of the health impairment and on the prevailing contextual factors, such care is provided on an hourly or daily basis for one or up to several weeks.Thereby, the workplace attendant refers the client to the most reasonable and gentle mode of working that is possible. Moreover, the workplace attendant takes care of potential difficulties arising during the stage of resuming employment, in order to work out strategies early on for ensuring the success of rehabilitation and for transferring such strategies to operational practice, jointly with all parties involved, including the BGW rehab managers.The potential need for qualification, following the motto of “Occupational placement comes before qualification”, is thereby identified at an early stage and in a purposeful way.Principal Experience and Summary: Workplace care facilitates highly practice‐oriented reintegration; Difficulties in the reintegration process are identified at an early stage; Workplace accompaniment gears prevention very effectively to rehabilitation. Clients as well as employers perceive such support provided by the BGW as being sensible and helpful.
The role of employers in retaining older workers
Authors: Howe Caroline 
Published: November 2014

Background: Previous research into the retirement experience tends to focus on the individual experience. This study looks at the role of work and retirement on subjective wellbeing and how corporations must change to engage workers for longer if they themselves are to remain sustainable.Objectives: The objective of the study was to ascertain the need for creative solutions for older employer engagement.Method: Using mixed methods Internet-based research methodology, 317 people were recruited with an online survey. Thematic content analysis was utilised to explore trends that emerged from the qualitative analysis.Findings: The principle finding was that, employers need to rethink how they engage the older worker to ensure that their workforce remains stable.Discussion: Low levels of life satisfaction were linked with being dissatisfied at work. Being at work also decreased the likelihood of social interaction. In contrast retirement offered freedom from the constraints of work. Employers need to adopt suggested solutions around flexibility, offering choices and a differentiation of the meaning of the “working day” to this cohort if they are to be successful in retaining older workers in employment.Conclusion: The results are significant because governments, both local and federal, are encouraging an ageing workforce to remain in work longer. However employers need to consider how they can create greater autonomy for older people in the workplace to encourage them to want to work longer.
Improving injury outcomes and experience of indigenous New Zealanders
Authors: Witheford-Smith Suzanne , Karaitiana Ronald 
Published: November 2014

This presentation outlines disparities in the uptake of accident compensation entitlements by indigenous New Zealanders and a programme to prevent injury and improve outcomes/experience for Māori. ACC administers NZ's 24/7 no-fault accident compensation scheme which covers New Zealanders and visitors to NZ injured at work, home, play, in road crashes, or during treatment. Research has shown that disparities persist in injury outcomes/experience for NZ's indigenous population (Māori make-up 15% of the population and experience multiple disadvantage). This is inconsistent with the Government's commitment to Crown-Māori partnership.Disparities findings persist for Māori included; higher rates of serious/fatal injuries on the roads, lower GP referral rates to medical/surgical specialists, found the claim process more complicated and ACC less helpful in their return to work and lower rates of employment participation following a serious injury.ACC's 10 year vision of success; improved outcomes/experience for Māori clients and businesses, increased injury prevention effectiveness for Māori and improved ACC culture and capability in relation to Māori.Over a three year period, the ACC Board considered a series of papers on disparities in the uptake of entitlements by Māori. In early 2013, the Board considered advice on the Scheme's and organisation's responsiveness to Māori and endorsed the need for significant improvements. We shall explore two phases. Phase 1 of ACC's Māori Programme aims to reduce disparities, build organisational capability and establish relationships. Initiatives include innovative solutions to improve employment participation of seriously injured Māori. Phase 2 builds new partnerships with indigenous organisations and embeds cultural-responsiveness into the organisation/system. Challenges include building genuine partnerships and sustaining the Programme in a changing organisational, social, political and economic context. Improving injury outcomes/experience for Māori is a long-term commitment. Programme success includes fewer fatal/serious injuries to Maori, better employment participation after injury, and new partnerships.
Case studies of employers with exemplary early intervention and return to work practices
Authors: Randall Christine 
Published: November 2014

Background: There is ample evidence that returning to suitable work is good for an injured worker's health and wellbeing. Evidence from the 2013 National Return to Work Survey shows a positive relationship between employers’ treatment of injured workers and return to work outcomes.Objectives: The case studies project aims to provide:
•employers with examples of practical solutions, practices or systems to improve return to work outcomes, and•policy makers with a summary of key themes and best practice approaches to inform the development of policy and programs.Methods: Organisations for the case studies will be shortlisted from recent Australian national return to work award winners and finalists. Key players within the organisations, including managers, supervisors, rehabilitation co-ordinators and recovered injured workers, will be interviewed to obtain information and attitudes on the early intervention and return to work approaches. An analysis of the case studies and a literature review on current best practice for early intervention and return to work will be conducted from which key themes and practices will be identified.Findings: The project will produce two reports: case studies of six organisations’ practices, and an analytical report on key findings and best practice for early intervention and return to work. The case studies will provide employers and workers published examples of practical approaches to improving return to work outcomes. The case studies also explore the roles and views of management, supervisors and injured workers. The analytical report will examine the case studies and report on key themes and best practice, providing policy makers and employers with an analysis of findings. The 2013 National Return to Work survey provides supporting evidence of the positive relationship between return to work outcomes and a range of variables related to the role of the employer and the workplace.Conclusion: It is anticipated the case studies project will provide examples of practical approaches to improving early intervention and return to work outcomes, which other organisations may consider implementing.
What is the level of influence that may be exercised by income security organisations in facilitating employment outcomes?
Authors: Wiltshire Simon 
Published: November 2014

The primary function of income security organisations is to administer personal injury claims in a commercially viable and cost effective manner, while supporting the receivers of benefits/claimants to achieve a meaningful and durable return to employment. Given the pressures of commercial success in a competitive market measured by effective cost minimisation, to what extent is the level of support offered to individuals claiming benefits impacted by an organisation's purpose to be profitable? The extent of influence exercised by income security organisations in administering claims and driving employment outcomes is limited by jurisdictional legislation and regulations. Furthermore, the requirements to meet compliance requirements can often be detrimental to the quality of management of claims, resulting in a highly administrated framework. This can restrict an organisations capacity to meet individual needs, especially in cases that are not seen as fitting the norm. Considering the conflicting demands imposed by commercial pressures and regulatory frameworks, what is the level of influence that may be exercised by these organisations in facilitating employment outcomes? Employers Mutual have maintained a leading position in the NSW Workers Compensation Scheme, achieving commercial targets, meeting legislative requirements and promoting excellence in employment outcomes through:
•Organisational vision – we help people get their lives back;•Focus on support and development of people as front line drivers of performance;•Role design – how we actually do our jobs;•Implementing organisation strategies and designing key support roles aligned to remunerative measures;•Strategic focus on early intervention to facilitate return to work;•Ensuring people receive the full extent of their entitlements, however, are not compensated to excess;•Service level agreements with employers/brokers with a key focus on return to work expectations;•Strong relationships with third party service providers; and•A structured framework that allows flexibility in decision making.
Breaking the web of needless disability
Authors: Aurbach Robert 
Published: November 2014

Background: In 2012 I presented a program to the London IFDM entitled “Breaking the Web of Needless Disability”. The model drew heavily from the latest neuroscientific theory, and was quite well received. This program represents further development of the ideas presented in London to encompass “resilience”.Hypothesis: 1. “Resilience” is a behaviour that is learned and unlearned like any other skill; 2. Understanding how humans think and react in compensation systems will yield valuable insight into the building of resilience to unnecessary disability.Methods: Extension of existing literature and pilot studies examining the differences between those people who suffer injury and recover as expected and those who suffer the same sort of injury and spiral down into despair and dependency. Principal findings: “Resilience” is a specific behaviour beneficially affecting the sense of loss of control that attends injury. There are four specific “styles” of resilience. Each person uses one or more of these styles to a greater or lesser degree. By use of modified psychological inventory tools, the predominant resilience style of an individual can be ascertained, and approaches that enhance their resilience style can be utilised for either prevention or treatment.Discussion: “Habits of thought” are established by repetition. For habits of thinking of one's self as able or disabled, the most significant form of repetition is internal dialog. In resilient people, there are four discrete kinds of dialog that are used to overcome a sense of loss of control. By identifying and bolstering those styles of resilience an injured person can become less susceptible to a sense of loss of control that gives rise to unnecessary disability.Conclusion: The approach is useful in understanding failure to recover as expected, and shows promise for effective individualised treatment for injury management.
How does self-regulation of emotions impact employee work engagement: The mediating role of social resources
Authors: Bouckenooghe Dave , Raja Usman , Abbas Muhammad 
Published: November 2014

AbstractDrawing upon the Conservation of Resources Theory, we investigated the hitherto unexplored role of ‘social resources’ (i.e., trust in supervisor and social interaction) in mediating the relationship between ‘self-regulation of emotions’ (i.e., a personal resource) and work engagement. The data were collected from 296 IT professionals at four well-established IT firms in Ukraine. As we hypothesized, self-regulation of emotions positively affected work engagement, yet this effect partially disappeared when controlling for the role of social resources. Together, these findings illustrate the dynamic role of an individual's personal and social resources in fostering work engagement.