bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anthocyanin Phytochemical Profiles and Anti‐oxidant Activities of Vitis candicans and Vitis doaniana
Authors: la Cruz Alexander Acevedo De , Hilbert Ghislaine , Mengin Virginie , Rivière Céline , Ollat Nathalie , Vitrac Caroline , Bordenave Louis , Decroocq Stéphane , Delaunay Jean‐Claude , Mérillon Jean‐Michel , Monti Jean‐Pierre , Gomès Eric , Richard Tristan 
Published: July 2013

ABSTRACTIntroductionGrapes are one of the most important fruit crops in the world. The quality of red grape berries greatly depends on skin colour, mainly due to the anthocyanin profile. Today, the American Vitis species have the greatest potential for breeding work. They have multiple resistance properties in comparison with Vitis vinifera but little is known about their anthocyanin content.ObjectiveTo determine the anti‐oxidant properties and anthocyanin profile of two American species, Vitis candicans and Vitis doaniana, by using LC–MSn and LC–NMR.MethodsGrape extracts were prepared by extraction of berry skins with acidified methanol. The complete structure elucidation of the individual anthocyanins was performed with LC–MSn, LC–NMR and NMR experiments. Individual anthocyanins in the extracts were quantified by using malvidin glucoside as external standard. The anti‐oxidant activities of grape skin extracts were evaluated by using 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH•) radical scavenging and oxygen radical absorbance capacity (ORAC) assays.ResultsBy using LC–MSn and LC–NMR experiments, 30 anthocyanins were identified and quantified in the two Vitis species, including two new cis‐p‐coumaroyl derivatives. Vitis candicans and V. doaniana showed significant differences in their anthocyanin profile. These two Vitis species possess low‐to‐medium anti‐oxidant activities in comparison with V. vinifera.ConclusionThe profiles of 30 anthocyanins were established unambiguously in two American Vitis species. Copyright © 2013 John Wiley & Sons, Ltd.
Characterisation of Diarylheptanoid‐ and Flavonoid‐type Phenolics in Corylus avellana L. Leaves and Bark by HPLC/DAD–ESI/MS
Authors: Riethmüller Eszter , Alberti Ágnes , Tóth Gergő , Béni Szabolcs , Ortolano Francesco , Kéry Ágnes 
Published: July 2013

ABSTRACTIntroductionThe leaves of Corylus avellana L. (common hazel, Betulaceae), a plant with a wide distribution in Europe, have been used in folk medicine for various diseases, but phytochemical exploration of C. avellana is still incomplete. To the best of our knowledge there is no previous report concerning diarylheptanoids in C. avellana, although these compounds show a frequent occurrence among Betulaceae plants.ObjectiveTo improve existing online chromatographic methods for the investigation of the phenolic compounds in C. avellana leaves and bark, focusing on diarylheptanoid‐type molecules.MethodsDried and powdered leaves and bark of C. avellana were extracted with increasing polarity solvents (n‐hexane, chloroform, ethyl acetate and methanol) in Soxhlet extractor apparatus. For the characterisation of the phenolic compounds in the ethyl acetate and methanolic extracts, UV spectral data, obtained by LC with a diode‐array detector (DAD), accurate molecular mass and formula, acquired by LC and electrospray ionisation (ESI) with time‐of‐flight (TOF) MS and fragmentation pattern, given by LC–ESI/MS/MS analyses were used. Quantitation of the compounds was performed by LC–MS/MS.ResultsIn the methanolic and ethyl acetate extracts of C. avellana bark four flavonoid glycosides and a caffeoyl hexoside derivative were detected and characterised, while in C. avellana leaves, seven diarylheptanoid‐type molecules were tentatively identified in addition to six flavonoid components. As far as we know this is the first study where the presence of diarylheptanoids in C. avellana is reported.ConclusionThe improved HPLC/DAD–ESI/MS method was successfully utilised for the characterisation and quantitation of the phenolic compounds in C. avellana bark and leaves extracts. Copyright © 2013 John Wiley & Sons, Ltd.
Fraction of cell‐free fetal DNA in the maternal serum as a predictor of abnormal placental invasion‐a pilot study
Authors: Samuel Amber , Bonanno Clarissa , Oliphant Arnold , Batey Annette , Wright Jason D. 
Published: July 2013

ABSTRACTObjectivePilot studies have suggested the amount of cell‐free fetal DNA (cffDNA) in the maternal serum is increased in pregnancies complicated by placenta accreta. Our objective was to determine if levels of cffDNA can predict invasive placentation.MethodsWe enrolled women with antenatally suspected placenta accreta compared with gestational age‐matched cases of placenta previa and women with prior cesarean deliveries (CDs) and normal placentation. The fetal fraction of cffDNA was quantified using DANSR™ as compared with total DNA. Patient characteristics were compared between the three groups using ANOVA, and linear regression was used to compare the fraction of cffDNA between pathologically confirmed cases of placenta accreta, placenta previa and normal placentation.ResultsTwenty women were enrolled, (seven cases of placenta accreta, six cases of placenta previa and seven cases of normal placentation with prior CD). The groups did not differ by maternal weight, placental weight, number of prior CD or years from prior CD. The mean fraction of cffDNA did not differ significantly by group when controlling for the aforementioned factors (accreta = 19.1%, previa = 27.2%, prior CD = 28.9%, p = 0.26), nor did the median (accreta = 17.0%, previa = 30.1%, prior CD = 22.7%).ConclusionsWe could not confirm diagnostic benefit. Further investigation of other biomarkers including placental mRNA is warranted. © 2013 John Wiley & Sons, Ltd.
Noninvasive prenatal diagnosis experience in the Çukurova Region of Southern Turkey: detecting paternal mutations of sickle cell anemia and β‐thalassemia in cell‐free fetal DNA using high‐resolution melting analysis
Authors: Yenilmez Ebru Dündar , Tuli Abdullah , Evrüke İ. Cüneyt 
Published: July 2013

ABSTRACTObjectiveThis study used a high‐resolution melting (HRM) technique to detect paternal mutations for the noninvasive prenatal diagnosis (NIPD) of β‐thalassemia and sickle cell anemia (HbS). We also determined the levels of cell‐free fetal DNA and total cell‐free DNA.MethodsWe used the HRM technique for fetal genotyping of paternal mutations in maternal plasma from 32 pregnancies at risk of β‐thalassemia and 57 pregnancies at risk of HbS. The DNA levels in maternal plasma were measured using real‐time quantitative PCR. Multiples of the median (MoM) values were calculated in women at risk for β‐thalassemia or HbS.ResultsTwenty‐two paternal mutations were detected in 89 pregnant women. Although we were successfully able to detect the paternal β‐thalassemia mutations, the mutant HbS fetuses could not be distinguished from maternal background in the early weeks of pregnancy. The detection of DYS14 in male fetuses was 100%. The MoM values of women at high risk of having HbS‐affected fetuses were higher than those for the other groups.ConclusionHigh‐resolution melting is a useful method for NIPD of β‐thalassemias by detecting paternal mutations in the maternal plasma. Cell‐free fetal DNA quantification and MoM values were not informative for HbS or β‐thalassemias in early pregnancy. © 2013 John Wiley & Sons, Ltd.
Assessment of fruit fly (Diptera: Tephritidae) management practices in deciduous fruit growing areas in South Africa
Authors: Manrakhan Aruna , Addison Pia 
Published: July 2013

AbstractBACKGROUNDCeratitis capitata (Wiedemann) and Ceratitis rosa Karsch are important fruit fly pests of deciduous fruit in the Western Cape Province, South Africa. The main techniques used for fruit fly control in the Western Cape are the sterile insect technique (SIT) targeting C. capitata and the bait application technique (BAT). We determined the relative success of SIT by comparing adult fly‐trap catches and fruit infestation in commercial orchards between three regions under SIT and two regions under BAT in the Western Cape, from 2006 to 2008.RESULTSCeratitis capitata was predominant in all regions. In commercial orchards, C. capitata catches peaked towards the end of the fruiting season (March to May) and were low between July and January. During the late season, C. capitata catches were significantly higher in two of the regions under SIT. The sterile to wild male ratio in those regions was found to be mostly <1.CONCLUSIONSIT is not being properly applied in some regions. SIT should be implemented when the pest population is low. The sterile to wild fly ratios should be increased. Alternatively, BAT should be used to lower the pest population before SIT application. Control methods should be more integrated and applied area‐wide. © 2013 Society of Chemical Industry
WETLAND HABITAT PATCHES AS ECOLOGICAL COMPONENTS OF LANDSCAPE MEMORY IN A HIGHLY MODIFIED FLOODPLAIN
Authors: Ortmann‐Ajkai A. , Lóczy D. , Gyenizse P. , Pirkhoffer E. 
Published: July 2013

ABSTRACTLandscape memory and ecological memory are relatively new concepts developed independently in geomorphology and ecology. Eco‐geomorphology strives to enrich the concept of landscape memory with that of ecological memory, as ecosystems build inherent parts of landscapes and are controlled by geomorphological factors. This integration is illustrated by a small‐scale eco‐geomorphological case study of links between ecosystems and their hydrogeomorphological template, in the floodplain of a medium size Hungarian River. Despite that the river is channelized by 1835 and most of the study area is under intensive agricultural cultivation, the seminatural vegetation along the various reaches shows significant variations according to geomorphological background variables: detrended correspondence analysis yields results of p = 0.002 significance. Our results prove that geomorphological patterns in floodplains, defined by landscape memory, and the related vegetation patterns, as manifestations of ecological memory, jointly determine the face of floodplain landscapes. The integration of ecological memory into the definition of landscape memory may help meet the challenges of eco‐geomorphology and facilitate a holistic understanding of landscapes, and thus be instrumental for reconstructing past environments—both in theory (scientific research) and practice (rehabilitation projects). Copyright © 2013 John Wiley & Sons, Ltd.
A novel discrete wavelet transform based on traveling wave technique for identifying the fault location for transmission network systems
Authors: Patcharoen Theerasak , Ngaopitakkul Atthapol 
Published: July 2013

AbstractThis paper proposes a technique to identify fault location on transmission systems using discrete wavelet transforms (DWTs). Fault conditions are simulated using alternative transients program/electromagnetic transients program (ATP/EMTP) in order to obtain the current signals. Various cases based on Thailand electricity transmission systems are studied to verify the validity of the proposed technique. The comparisons among the maximum coefficients in first scale of all buses that can detect fault are performed to detect the faulty bus. The first peak time of positive sequence current obtained from the faulty bus is used as input data for the traveling wave equation. It is shown that the proposed technique gives satisfactory accuracy and is suitable for all types of fault occurring in different sections of transmission lines. © 2013 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
Morphometric alterations, steatosis, fibrosis and active caspase‐3 detection in carbamate bendiocarb treated rabbit liver
Authors: Petrovova Eva , Purzyc Halina , Mazensky David , Luptakova Lenka , Torma Norbert , Sopoliga Igor , Sedmera David 
Published: July 2013

ABSTRACTIncreasing use of pesticides all over the world makes it necessary to reveal the toxic risk in populations of nontargeted organisms. Bendiocarb is one of the 12 insecticides recommended by the World Health Organization for use in malaria control in Africa, and is used against a variety of insects. The liver has an important role in its process of detoxication and excretion. In our experiment 56 adult rabbits of breed HY+, 28 males and 28 females were used. Animals were divided into groups (control, days 10, 20, 30 of bendiocarb administration). The presence of many binucleated hepatocytes, the highest number of liver cells and their decreased size at 10 day after bendiocarb administration was observed as an evidence of the hepatic regeneration. After the long‐term treatment pronounced changes were presented such as vacuolization and dilatation of hepatocytes, dilatation of sinusoids between hepatocytes, and focal infiltration of inflammatory cells. Numerous cells with caspase‐3 activity were present throughout the organ, most commonly around the portal tract and close to the central vein. Short and long‐term bendiocarb treatment showed the central vein thickened rim with increased deposition of collagen, spreading of collagen fibers into the perisinusoidal, and pericellular space surrounding the central veins, and septal fibrosis extended from the portal tract. Subsequently, presence of the lipid vacuoles both in the liver parenchyma and inner of the hepatocytes were observed. These results suggest that bendiocarb treatment leads to increased cell death, liver perisinusoidal fibrosis, and steatosis, especially during the long‐term administration. © 2013 Wiley Periodicals, Inc. Environ Toxicol 30: 212–222, 2015.
Prepyramidal clustering and Robinsonian dissimilarities: one‐to‐one correspondences
Authors: Bertrand Patrice , Diatta Jean 
Published: July 2013

Cluster analysis and seriation are basic data mining techniques. We consider here clustering structures that enable us to achieve both a clustering and a seriation, namely the hierarchical, pyramidal, and prepyramidal clustering structures. Cluster collections of these types determine seriations of any data set by providing compatible orders, i.e., total rankings of the whole data set for which the objects within each cluster are consecutive. Moreover, the dissimilarity measures induced from such cluster collections are Robinsonian; in other words, the more distant the objects in a compatible order, the higher the induced dissimilarity value. It results in a one‐to‐one correspondence between weakly indexed pyramids and the class of Robinsonian dissimilarities, which holds still in a general setting where undistinguishable objects can be detected, and, as shown in this paper, extends, to prepyramids, which are not required to contain arbitrary intersections of clusters.This article is categorized under:

Technologies > Structure Discovery and Clustering
An expanded tool kit for the auxin‐inducible degron system in budding yeast
Authors: Morawska Magdalena , Ulrich Helle D. 
Published: July 2013

AbstractFusion of inducible degradation signals, so‐called degrons, to cellular proteins is an elegant method of controlling protein levels in vivo. Recently, a degron system relying on the plant hormone auxin has been described for use in yeast and vertebrate cells. We now report the construction of a series of vectors that significantly enhance the versatility of this auxin‐inducible degron (AID) system in Saccharomyces cerevisiae. We have minimized the size of the degron and appended a series of additional epitope tags, allowing detection by commercial antibodies or fluorescence microscopy. The vectors are compatible with PCR‐based genomic tagging strategies, allow for C‐ or N‐terminal fusion of the degron, and provide a range of selection markers. Application to a series of yeast proteins, including essential replication factors, provides evidence for a general usefulness of the system. © 2013 The Authors. Yeast published by John Wiley & Sons, Ltd.
What Factors Influence 10‐year Survival After Curative Resection of a Colorectal Carcinoma?
Authors: Ueberrueck Torsten , Wurst Christine , Rauchfuß Falk , Knösel Thomas , Settmacher Utz , Altendorf‐Hofmann Annelore 
Published: July 2013

AbstractBackgroundTen‐year survival rates are only rarely reported and frequently include a large proportion of censored data—that is, most of the patients have not survived the 10 years. We therefore selected patients in a prospectively maintained, hospital‐based tumor register who had been operated on for colorectal carcinoma (CRC) more than 10 years earlier and who were classified as long‐term survivors.MethodsFor 589 consecutive CRC patients who underwent R0 resection in the period 1990–1998, we compared prognosis‐relevant characteristics and calculated the survival rate as a function of age, sex, location of the tumor, general state of health, urgency of the operation, and pT and pN class. All patients were observed until their death or until at least 10 years after resection. Patients who died of other causes were censored. Overall survival and relative survival (the latter based on tumor‐related death) were assessed.ResultsThe 10‐year survivors were more often female (not significant), younger (p < 0.001), in good general health (p < 0.001), had undergone elective resection (p < 0.001), and had early‐stage tumors (p < 0.001). In the univariate analysis emergency operation, impaired general health, invasion beyond the muscularis propria, and lymph‐node metastasis were found to reduce relative survival. In the multivariate analysis, location, emergency resection, pT, and pN were found to be statistically independent risk factors.ConclusionsLong‐term freedom from tumor recurrence, like‐short‐term, is influenced largely by factors that are beneficially influenced by early recognition. The patient’s age at resection is immaterial.
Temperature Dependency When Generating Glycidyl and 3‐MCPD Esters from Diolein
Authors: Shimizu Masao , Weitkamp Petra , Vosmann Klaus , Matthäus Bertrand 
Published: July 2013

AbstractA series of heating tests of pure diolein with and without the addition of tetrabutylammonium chloride as a chloride source were conducted at temperatures that ranged from 180 to 240 °C. The levels of glycidyl ester (GE) and 3‐monochloropropane‐1,2‐diol (3‐MCPD) ester reached plateau levels that depended on the heating temperature. Higher temperatures resulted in higher levels for both esters. A two‐step heating test with a combination of different temperatures revealed that the plateau levels of GE came from steady states between the generation and the transformation of the esters, and was controllable by temperature. Levels of GE were decreased at low temperatures followed by treatment at high temperatures. To the contrary, the plateau levels of 3‐MCPD esters seemed to have originated from the completion of the reactions that were caused by the shortage of an available chloride source and by the stable nature of the esters. The generation of 3‐MCPD esters was difficult to control, since the reactions were completed at an early stage of the heating period. Using raw materials containing low levels of precursor, such as partial acylglycerols and chloride, would be effective in mitigating the formation of the 3‐MCPD esters.
A systematic review on keystroke dynamics
Authors: Pisani Paulo Henrique , Lorena Ana Carolina 
Published: July 2013

Abstract
Computing and communication systems have improved our way of life, but have also contributed to an increased data exposure and, consequently, to identity theft. A possible way to overcome this issue is by the use of biometric technologies for user authentication. Among the possible technologies to be analysed, this work focuses on keystroke dynamics, which attempts to recognize users by their typing rhythm. In order to guide future researches in this area, a systematic review on keystroke dynamics was conducted and presented here. The systematic review method adopts a rigorous procedure with the definition of a formal review protocol. Systematic reviews are not commonly used in artificial intelligence, and this work contributes to its use in the area. This paper discusses the process involved in the review along with the results obtained in order to identify the state of the art of keystroke dynamics. We summarized main classifiers, performance measures, extracted features and benchmark datasets used in the area.
DSM-IV-TR Axes-I and II mental disorders in a representative and referred sample of unemployed youths – Results from a psychiatric liaison service in a job centre
Authors: Reissner V. , Mühe B. , Wellenbrock S. , Kuhnigk O. , Kis B. , Dietrich H. , Hebebrand J. 
Published: July 2013

AbstractObjectives:Increased levels of anxiety, depression and alcohol abuse are associated with unemployment. This study compares both DSM-IV-TR Axis-I and Axis-II mental disorders between a representative and a referred sample of unemployed youths aged 16.0 to 24.9.Methods:One hundred subjects were randomly recruited on the premises of the vocational services centre in the urban region of Essen, Germany (representative sample, RS). One hundred and sixty-five subjects constituting the ‘clinical sample’ (CS) were preselected and referred by case managers to the on-site psychiatric liaison service. Structured Clinical Interviews for DSM-IV (SCID-I and -II), measures of psychopathology and health service utilization were administered.Results:Ninety-eight percent and 43% of CS and RS subjects fulfilled DSM-IV criteria for mental disorders. Mood-, anxiety- and substance-related disorders were the most common Axis-I disorders in both samples. Personality disorders were diagnosed significantly more frequently in the CS. Despite the more severe psychopathology in subjects with mental disorders from the CS compared to the RS, no differences were found for recent mental health service utilisation.Conclusion:Because the sample of unemployed youths referred by case managers was significantly more disturbed in psychiatric terms, such a pre-selection is deemed useful in conjunction with a psychiatric liaison service on the premise of a job centre.
Bi-stability of a pendular disk in laminar and turbulent flows
Authors: Obligado M. , Puy M. , Bourgoin M. 
Published: July 2013

AbstractThe simple pendulum remains one of the most fundamental systems studied in physics. It is commonly used as a model to illustrate a broad variety of mechanisms in a wide range of areas. However, in spite of this popularity, subtle behaviours still remain to be discovered and to be explored when the pendulum is strongly coupled to fluid mechanics. This is for instance illustrated in recent studies by Neill, Livelybrooks & Donnelly (Am. J. Phys., vol. 75, 2007, pp. 226–229) and Bolster, Hershberger & Donnelly (Phys. Rev. E, vol. 81, 2010, pp. 1–6) which highlight the impact on a simple spherical pendulum of vortex shedding and added mass effects. In the present work we show that the equilibrium of a pendular disk facing a flow exhibits bi-stability and hysteresis. We give a simple interpretation of this behaviour in terms of a two-potential-well description, only requiring to know the angular dependence of the normal drag coefficient of an inclined plate. We investigate the influence of turbulence on the equilibrium of the pendulum in general and on the observed bi-stability in particular. Our results have potentially important fundamental and practical consequences: (i) they extend the attractiveness of the pendulum as a model to investigate generic questions related to bi-stable stochastic processes, (ii) they highlight important fluid dynamic mechanisms, including turbulent drag enhancement and fluid–structure interaction issues.