bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacts of survival and reproductive success on the long-term population viability of reintroduced great bustards Otis tarda in the UK
Authors: Ashbrook Kate , Taylor Andrew , Jane Louise , Carter Ian , Székely Tamás 
Published: June 2015

AbstractReintroductions aim to re-establish species within their historical ranges through the release of wild- or captive-bred individuals following extirpation (or extinction) in the wild. There is no general agreement on what constitutes a successful reintroduction but the probability of the population achieving long-term persistence should be addressed. Here we review a 10-year trial reintroduction of the great bustard Otis tarda, a globally threatened bird species, to the UK and assess the long-term population viability. Despite changes in rearing and release strategy, initial post-release survival probability remained consistently low, with only 11.3% of bustards (n = 167) surviving from release to 1 year post-release. Nineteen breeding attempts were made by eight females; however, only one chick survived > 100 days after hatching, and no wild juveniles have recruited into the population. Using demographic rates from the UK population and wild populations elsewhere, and stochastic population modelling, we investigate the viability of this reintroduced population by predicting population size over the next 10 years. Under current demographic rates the population was predicted to decline rapidly. Self-sufficiency was predicted only using the highest estimates from the UK population for first-year and adult survival, and recruitment rates from wild populations elsewhere. Although changes have been made in rearing, release strategies, habitat management and release sites used, these changes appear to have a modest effect on long-term viability. Substantial improvements in survival rates and productivity are necessary to establish a viable great bustard population in the UK, and we consider this unlikely.
Seed dispersal by rodents in a lowland forest in central Panama
Authors: Dittel Jacob W. , Lambert Thomas D. , Adler Gregory H. 
Published: June 2015

Abstract:We studied the removal of seeds of three species of large-seeded tree (Astrocaryum standleyanum, Attalea butyraceaandDipteryx oleifera) from three different heights within six study plots in a lowland forest in central Panama. Fresh fruits with intact seeds fitted with industrial sewing bobbins were placed within semi-permeable exclosures. Removed seeds were tracked to deposition sites, and seed fate was determined. Removals were likely perpetrated by two small rodents, the strictly terrestrialProechimys semispinosusand the scansorialSciurus granatensis, because they were the most abundant small rodents in the study site during the study period and were of sufficient size to remove large seeds. Rodent abundance and fruit availability were estimated by conducting censuses. Nine microhabitat variables were measured at each deposition site to determine if these two rodents were preferentially depositing seeds in sites with certain characteristics or were randomly depositing seeds. During the study, rodents handled 98 seeds, 85 of which were not predated upon and could potentially germinate. Removal rates were not influenced by rodent abundance or fruit availability. Seeds were most frequently moved <3 m and deposited with the fruit eaten and the seed intact. However, some seeds did experience relatively long-distance dispersal (>10 m). Rodents preferentially deposited seeds in locations with large logs (>10 cm diameter), dense herbaceous cover, and an intact canopy. The number of large logs was different from random locations. Despite not being able to determine long-term fate (greater thanc. 1 y), we show that these small rodents are not primarily seed predators and may in fact be important mutualists by dispersing seeds relatively long distances to favourable germination sites.
Trend of Japanese encephalitis in Uttar Pradesh, India from 2011 to 2013
Authors: JAIN P. , SINGH A. K. , KHAN D. N. , PANDEY M. , KUMAR R. , GARG R. , JAIN A. 
Published: June 2015

SUMMARYAs indicated by the sporadic Japanese encephalitis (JE) cases reported from the districts of Uttar Pradesh (UP), India, the disease is endemic in the state despite the fact that a JE vaccination programme has been ongoing in the state since 2006. Hence, the present study was undertaken to study the annual trend of JE in UP during January 2011 to December 2013. CSF and/or serum samples collected from acute encephalitis syndrome (AES) cases were referred to the virology laboratory at King George's Medical University, Lucknow and were tested for anti-JEV IgM antibodies by JEV MAC-ELISA kit. The study reveals that 26·9%, 9·9% and 14·8% of AES cases were positive for anti-JEV IgM in the years 2011, 2012 and 2013, respectively. Of the total JE confirmed cases, 30% were adults. Males were more commonly affected than females. A distinct peak of JE was seen in the monsoon and post-monsoon season, although sporadic cases were also reported in other months. JE vaccination by district in UP is discussed. This study reports that the proportion of JE positives in AES cases is decreasing in UP although the number of AES cases has not decreased. The study also discusses the probable causes of this decrease, including JE vaccination and natural periodicity due to herd immunity.
Does the weather play a role in the spread of pandemic influenza? A study of H1N1pdm09 infections in France during 2009–2010
Authors: VITTECOQ M. , ROCHE B. , COHEN J.-M. , RENAUD F. , THOMAS F. , GAUTHIER-CLERC M. 
Published: June 2015

SUMMARYUnderstanding patterns of influenza spread and persistence is crucial for pandemic preparedness. The H1N1pdm09 virus caused the first influenza pandemic of the 21st century which resulted in at least 18500 deaths. Based on laboratory-confirmed primary-care case reports we investigated the role of weather conditions and socio-demographic variables in its initial spread and subsequent presence in France. Our findings suggest that low relative humidity and high population density were determinants in shaping the early spread of the virus at the national level. Those conditions also favoured the persistence of viral presence throughout the first 33 weeks of the pandemic. Additionally this persistence was significantly favoured by low insolation. These results confirm the increasingly recognized role of humidity in influenza dynamics and underlie the concomitant effect of insolation. Therefore climatic factors should be taken into account when designing influenza control and prevention measures.
Within-herd prevalence thresholds for the detection of Mycobacterium avium subspecies paratuberculosis-positive dairy herds using boot swabs and liquid manure samples
Authors: DONAT K. , HAHN N. , EISENBERG T. , SCHLEZ K. , KÖHLER H. , WOLTER W. , ROHDE M. , PÜTZSCHEL R. , RÖSLER U. , FAILING K. , ZSCHÖCK P. M. 
Published: June 2015

SUMMARYThe control of Johne's disease requires the identification of Mycobacterium avium ssp. paratuberculosis (MAP)-positive herds. Boot swabs and liquid manure samples have been suggested as an easy-to-use alternative to sampling individual animals in order to diagnose subclinical Johne's disease at the herd level, but there is a need to evaluate performance of this approach in the field. Using a logistic regression model, this study aimed to calculate the threshold level of the apparent within-herd prevalence as determined by individual faecal culture, thus allowing the detection of whether a herd is MAP positive. A total of 77 boot swabs and 75 liquid manure samples were taken from 19 certified negative and 58 positive dairy herds. Faecal culture, three different polymerase chain reaction (PCR) methods and the combination of faecal culture with PCR were applied in order to detect MAP. For 50% probability of detection, a within-herd prevalence threshold of 1·5% was calculated for testing both matrices simultaneously by faecal culture and PCR, with the threshold increased to 4·0% for 90% probability of detection. The results encourage the use of boot swabs or liquid manure samples, or a combination both, for identifying MAP-positive herds and, to a certain extent, for monitoring certified Johne's disease-negative cattle herds.
Dissemination of clonally related multidrug-resistantKlebsiella pneumoniaein Ireland
Authors: MORRIS D. , O'CONNOR M. , IZDEBSKI R. , CORCORAN M. , LUDDEN C. E. , McGRATH E. , BUCKLEY V. , CRYAN B. , GNIADKOWSKI M. , CORMICAN M. 
Published: June 2015

SUMMARYIn October 2012, an outbreak of gentamicin-resistant, ciprofloxacin non-susceptible extended-spectrumβ-lactamase (ESBL)-producingKlebsiella pneumoniaeoccurred in a neonatal intensive care unit in Ireland. In order to determine whether the outbreak strain was more widely dispersed in the country, 137 isolates ofK. pneumoniaewith this resistance phenotype collected from 17 hospitals throughout Ireland between January 2011 and July 2013 were examined. ESBL production was confirmed phenotypically and all isolates were screened for susceptibility to 19 antimicrobial agents and for the presence of genes encodingblaTEM,blaSHV,blaOXA, andblaCTX-M; 22 isolates were also screened forblaKPC,blaNDM,blaVIM,blaIMPandblaOXA-48genes. All isolates harbouredblaSHVandblaCTX-Mand were resistant to ciprofloxacin, gentamicin, nalidixic acid, amoxicillin-clavulanate, and cefpodoxime; 15 were resistant to ertapenem, seven to meropenem and five isolates were confirmed as carbapenemase producers. Pulsed-field gel electrophoresis of all isolates identified 16 major clusters, with two clusters comprising 61% of the entire collection. Multilocus sequence typing of a subset of these isolates identified a novel type, ST1236, a single locus variant of ST48. Data suggest that two major clonal groups, ST1236/ST48 (CG43) and ST15/ST14 (CG15) have been circulating in Ireland since at least January 2011.
The prevalence of HSV-2 infection in HIV-1 discordant couples
Authors: DUAN S. , DING Y. , WU Z. , ROU K. , YANG Y. , WANG J. , GAO M. , YE R. , XIANG L. , HE N. 
Published: June 2015

SUMMARYWe aimed to investigate the prevalence and associated factors of HSV-2 discordance and concordance in HIV-1-discordant couples. This study used the baseline data from a cohort study of HIV-1-discordant couples in Dehong prefecture of Yunnan province, China. Of 954 participating couples, 42·4% were affected by HSV-2, of which 20·4% were HSV-2-concordant positive, 7·6% were HSV-2-discordant where the male was HSV-2 positive, and 14·4% were HSV-2 discordant where the female was HSV-2 positive. Compared to HSV-2-negative concordance, HSV-2 discordance with an HSV-2-positive male spouse was significantly associated with characteristics of the male spouse, including Han ethnicity and being in a second marriage. HSV-2 discordance with an HSV-2-positive female spouse was significantly associated with characteristics of the female spouse, including Han ethnicity, having engaged in commercial sex, having a sexual relationship of <3 years and being HIV-1 infected. Compared to HSV-2 discordance, HSV-2-positive concordance was significantly associated with an education level of middle school or higher for both spouses, a sexual relationship of ⩾3 years, more frequent sex and having an HIV-1-infected male spouse. The findings highlight the need for HSV-2 prevention and treatment efforts to reduce HSV-2 transmission in this population, and emphasize the importance of implementing prevention interventions early in couples' relationships.
Mucosal immunization of BALB/c mice with DNA vaccines encoding the SEN1002 and SEN1395 open reading frames of Salmonella enterica serovar Enteritidis induces protective immunity
Authors: BELLO J. , SÁEZ D. , ESCALONA E. , VELOZO P. , SANTIVIAGO C. A. , CONTRERAS I. , OÑATE Á. 
Published: June 2015

SUMMARYSalmonella Enteritidis is the main cause of foodborne salmonellosis worldwide. The limited effectiveness of current interventions against this pathogen has been the main incentive to develop new methods for the efficient control of this infection. To investigate the use of DNA vaccines against S. Enteritidis in humans, immune responses stimulated by two plasmids containing the genes designated SEN1002, located in the pathogenicity island SPI-19 and encoding a Hcp protein involved in transport mechanisms, and SEN1395, located in the genomic island ΦSE14 and encoding a protein of a new superfamily of lysozymes, were evaluated. Humoral and cellular responses following intranasal immunization of two groups of BALB/c mice with the plasmids pV1002 and pV1395 plus adjuvant were evaluated and it was observed that the IgG2a/IgG1 ratios were sixfold higher than control groups. Both plasmids stimulated specific secretory IgA production. Increased proliferation of lymphocytes and IFN-γ production were detected in both experimental groups. DNA-vaccinated mice developed protective immunity against a virulent strain of S. Enteritidis, with nearly 2 logs of protection level compared to the negative control values in the spleen. Therefore, DNA vaccines are efficient at stimulating cellular and humoral immune responses at systemic and mucosal levels.
A cluster of invasive meningococcal disease revealed by the characterization of a novel serogroup B meningococcal clone
Authors: VALINSKY L. , JAFFE J. , KELLER N. , BLOCK C. , ABRAMSON N. , STEIN-ZAMIR C. 
Published: June 2015

SUMMARYThe incidence of invasive infections due toNeisseria meningitidisin Israel is about 1/100 000 population annually. Three cases of meningococcal meningitis were reported in employees at a single plant; the first case appeared in March 2013 and the second and third cases appeared in December, almost 9 months later.N. meningitidisserogroup B was isolated from cerebrospinal fluid samples. Multilocus sequence typing assigned the three meningococcal isolates to ST10418, a new sequence type and a member of the ST32 clonal complex. The clonality was confirmed by performance of pulsed-field gel electrophoresis. Post-exposure antibiotic prophylaxis was administered to close contacts of the first case. Upon the diagnosis of the additional two cases, post-exposure prophylaxis was administered to all the plant employees. This report demonstrates the importance of combining public health measures and advanced laboratory studies to confirm clonality and to prevent further disease spread in a closed setting.
Wildlife conservation, labor supply and time values in rural Botswana
Authors: Larson Douglas M. , Pienaar Elizabeth F. , Jarvis Lovell S. 
Published: June 2015

AbstractTo improve wildlife conservation incentives in community-based natural resource management programs, a better understanding of rural communities' willingness to engage in wildlife conservation jobs is needed. We implement a discrete choice model explaining reservation wages for nine conservation jobs using contingent behavior data from rural Botswana residents. We present a model in which the conditional indirect utility function incorporates a more general value of time than has previously been used, and this specification outperforms the standard model. Sample estimates indicate that reservation wages are modestly higher for women than for men, and that residents have higher reservation wages for jobs requiring more exertion or involving more danger.
Mono-specific facies of Parazoanthus axinellae in Luiz Saldanha Marine Park
Authors: Gomes-Pereira J.N. , Vasco-Rodrigues N. , Afonso A. , Albuquerque M. , Berecibar E. , Bernardes L. , Calado A. , Castanheira A. , Tojeira I. , Tempera F. , Dias F.C. , Santos R.S. , Campos A.S. 
Published: June 2015

We report the first mono-specific facies ofParazoanthus axinellaefrom the western Portuguese coast. The habitat was recorded using a MiniRov Observer on a large outcrop at ca. 55–60 m in the Luiz Saldanha Marine Park. A method to estimate the maximum uncertainty of the ROV position is illustrated and the occurrence of the facies is discussed.
Specificity and catalysis hardwired at the RNA–protein interface in a translational proofreading enzyme
Authors: Ahmad Sadeem , Muthukumar Sowndarya , Kuncha Santosh Kumar , Routh Satya Brata , Yerabham Antony S.K. , Hussain Tanweer , Kamarthapu Venu , Kruparani Shobha P , Sankaranarayanan Rajan 
Published: June 2015

AbstractProofreading modules of aminoacyl-tRNA synthetases are responsible for enforcing a high fidelity during translation of the genetic code. They use strategically positioned side chains for specifically targeting incorrect aminoacyl-tRNAs. Here, we show that a unique proofreading module possessing a D-aminoacyl-tRNA deacylase fold does not use side chains for imparting specificity or for catalysis, the two hallmark activities of enzymes. We show, using three distinct archaea, that a side-chain-stripped recognition site is fully capable of solving a subtle discrimination problem. While biochemical probing establishes that RNA plays the catalytic role, mechanistic insights from multiple high-resolution snapshots reveal that differential remodelling of the catalytic core at the RNA–peptide interface provides the determinants for correct proofreading activity. The functional crosstalk between RNA and protein elucidated here suggests how primordial enzyme functions could have emerged on RNA–peptide scaffolds before recruitment of specific side chains.
Socio-economic position as an intervention against overweight and obesity in children: a systematic review and meta-analysis
Authors: Wu Shunquan , Ding Yingying , Wu Fuquan , Li Ruisheng , Hu Yan , Hou Jun , Mao Panyong 
Published: June 2015

AbstractStudies that investigated the association between socio-economic position (SEP) and obesity in children suggest inconsistent results. The aim of this study is to summarize and quantify the current evidence on SEP and risks of overweight and obesity in children aged 0–15 years. Relevant studies published between 1990 to Sep 4, 2014 were searched in Medline, Web of Science, Embase and the Cochrane Database of Systematic Reviews. Risk estimates from individual studies were pooled using random-effects models, according to lowest vs the highest SEP category. A total of 62 articles were included in the meta-analysis. The odds of both overweight risk and obesity risk were higher in the children with lowest SEP than in those with highest SEP (OR, 1.10, 95% CI: 1.03–1.17 and OR, 1.41, 95% CI: 1.29–1.55, respectively). Sub-group analyses showed that the inverse relationships between SEP and childhood overweight and obesity were only found in high-income countries and in more economic developed areas. In conclusion, our study suggests that children with lower SEP had higher risks of overweight and obesity and the increased risks were independent of the income levels of countries.
The Surtsey Magma Series
Authors: Schipper C. Ian , Jakobsson Sveinn P. , White James D.L. , Palin J. Michael , Bush-Marcinowski Tim 
Published: June 2015

AbstractThe volcanic island of Surtsey (Vestmannaeyjar, Iceland) is the product of a 3.5-year-long eruption that began in November 1963. Observations of magma-water interaction during pyroclastic episodes made Surtsey the type example of shallow-to-emergent phreatomagmatic eruptions. Here, in part to mark the 50thanniversary of this canonical eruption, we present previously unpublished major-element whole-rock compositions and new major and trace-element compositions of sideromelane glasses in tephra collected by observers and retrieved from the 1979 drill core. Compositions became progressively more primitive as the eruption progressed, with abrupt changes corresponding to shifts between the eruption’s four edifices. Trace-element ratios indicate that the chemical variation is best explained by mixing of different proportions of depleted ridge-like basalt, with ponded, enriched alkalic basalt similar to that of Iceland’s Eastern Volcanic Zone; however, the systematic offset of Surtsey compositions to lower Nb/Zr than other Vestmannaeyjar lavas indicates that these mixing end members are as-yet poorly contained by compositions in the literature. As the southwestern-most volcano in the Vestmannaeyjar, the geochemistry of the Surtsey Magma Series exemplifies processes occurring within ephemeral magma bodies on the extreme leading edge of a propagating off-axis rift in the vicinity of the Iceland plume.
Spider wrapping silk fibre architecture arising from its modular soluble protein precursor
Authors: Tremblay Marie-Laurence , Xu Lingling , Lefèvre Thierry , Sarker Muzaddid , Orrell Kathleen E. , Leclerc Jérémie , Meng Qing , Pézolet Michel , Auger Michèle , Liu Xiang-Qin , Rainey Jan K. 
Published: June 2015

AbstractSpiders store spidroins in their silk glands as high concentration aqueous solutions, spinning these dopes into fibres with outstanding mechanical properties. Aciniform (or wrapping) silk is the toughest spider silk and is devoid of the short amino acid sequence motifs characteristic of the other spidroins. Using solution-state NMR spectroscopy, we demonstrate that the 200 amino acid Argiope trifasciata AcSp1 repeat unit contrasts with previously characterized spidroins, adopting a globular 5-helix bundle flanked by intrinsically disordered N- and C-terminal tails. Split-intein-mediated segmental NMR-active isotope-enrichment allowed unambiguous demonstration of modular and malleable “beads-on-a-string” concatemeric behaviour. Concatemers form fibres upon manual drawing with silk-like morphology and mechanical properties, alongside secondary structuring and orientation consistent with native AcSp1 fibres. AcSp1 structural stability varies locally, with the fifth helix denaturing most readily. The structural transition of aciniform spidroin from a mostly α-helical dope to a mixed α-helix/β-sheet-containing fibre can be directly related to spidroin architecture and stability.
Musical training, individual differences and the cocktail party problem
Authors: Swaminathan Jayaganesh , Mason Christine R. , Streeter Timothy M. , Best Virginia , Kidd, Jr Gerald , Patel Aniruddh D. 
Published: June 2015

AbstractAre musicians better able to understand speech in noise than non-musicians? Recent findings have produced contradictory results. Here we addressed this question by asking musicians and non-musicians to understand target sentences masked by other sentences presented from different spatial locations, the classical ‘cocktail party problem’ in speech science. We found that musicians obtained a substantial benefit in this situation, with thresholds ~6 dB better than non-musicians. Large individual differences in performance were noted particularly for the non-musically trained group. Furthermore, in different conditions we manipulated the spatial location and intelligibility of the masking sentences, thus changing the amount of ‘informational masking’ (IM) while keeping the amount of ‘energetic masking’ (EM) relatively constant. When the maskers were unintelligible and spatially separated from the target (low in IM), musicians and non-musicians performed comparably. These results suggest that the characteristics of speech maskers and the amount of IM can influence the magnitude of the differences found between musicians and non-musicians in multiple-talker “cocktail party” environments. Furthermore, considering the task in terms of the EM-IM distinction provides a conceptual framework for future behavioral and neuroscientific studies which explore the underlying sensory and cognitive mechanisms contributing to enhanced “speech-in-noise” perception by musicians.