bluuebunny/crossref_metadata_2025_split huggingface dataset:
Geometric morphometrics analysis of forensically important Sarcophaga species
Authors: Reis Carina I C , Mascarenhas Paulo , Farinha Ana C , Menezes Marco A , Rebelo Maria Teresa 
Published: July 2024

Abstract
Geometric morphometrics is a method that offers a mathematical description of biological forms based on geometric definitions of their size and shape, enabling the discrimination between species. In this study, we aimed to utilize geometric morphometrics as a valuable tool for identifying Sarcophagidae species, a family of flies that are challenging to distinguish morphologically. We collected a total of 80 wings (both left and right) from male individuals representing nine species (Sarcophaga africa, S. argyrostoma, S. hirticrus, S. jacobsoni, S. lehmanni, S. melanura, S. pandellei, S. portschinskyi, and S. teretirostris). These wings were removed, mounted on microscope slides, photographed, and digitized using 15 landmarks. The results demonstrated effective differentiation among seven species, representing significant progress in the expedited identification of Sarcophaga species. Due to its speed, affordability, and user-friendly nature, the wing landmark-based geometric morphometrics, when combined with taxonomy, can enhance the robustness of Sarcophagidae analyses and prove useful for discriminating flesh flies in forensic contexts.
Genome-Wide Gene Birth–Death Dynamics Are Associated with Diet Breadth Variation in Lepidoptera
Authors: Dort Hanna , van der Bijl Wouter , Wahlberg Niklas , Nylin Sören , Wheat Christopher W 
Published: July 2024

Abstract
Comparative analyses of gene birth–death dynamics have the potential to reveal gene families that played an important role in the evolution of morphological, behavioral, or physiological variation. Here, we used whole genomes of 30 species of butterflies and moths to identify gene birth–death dynamics among the Lepidoptera that are associated with specialist or generalist feeding strategies. Our work advances this field using a uniform set of annotated proteins for all genomes, investigating associations while correcting for phylogeny, and assessing all gene families rather than a priori subsets. We discovered that the sizes of several important gene families (e.g. those associated with pesticide resistance, xenobiotic detoxification, and/or protein digestion) are significantly correlated with diet breadth. We also found 22 gene families showing significant shifts in gene birth–death dynamics at the butterfly (Papilionoidea) crown node, the most notable of which was a family of pheromone receptors that underwent a contraction potentially linked with a shift to visual-based mate recognition. Our findings highlight the importance of uniform annotations, phylogenetic corrections, and unbiased gene family analyses in generating a list of candidate genes that warrant further exploration.
Getting Nosy: Olfactory Rosette Morphology and Lamellar Microstructure of Two Chondrichthyan Species
Authors: Simonitis Lauren Eve , Clark Aubrey E , Barskaya Elizaveta , Castillo Gabriella , Porter Marianne , Meredith Tricia 
Published: July 2024

Synopsis
To smell, fish rely on passive water flow into their olfactory chambers and through their olfactory rosettes to detect chemical signals in their aquatic environment. The olfactory rosette is made up of secondarily folded tissues called olfactory lamellae. The olfactory morphology of cartilaginous fishes varies widely in both rosette gross morphology and lamellar microstructure. Previous research has shown differences in lamellar sensory morphology depending on the position along the rosette in hammerheads (family Sphyrnidae). Here, we investigate if this pattern continues in members of two other chondrichthyan families: Squalidae and Chimaeridae. Using contrast-enhanced microCT and scanning electron microscopy, we investigated patterns in lamellar morphology based on lamellar position along the olfactory rosette in Pacific spiny dogfish (Squalus suckleyi) and spotted ratfish (Hydrolagus colliei). We describe the gross olfactory rosette anatomy and lamellar microstructure of both species. We also put forth a new method, combining 3D morphological microCT data with 2D SEM microstructure data to better approximate lamellar sensory surface area. We found that in both species, lamellae in the center of the rosette were larger with more secondary folds. However, we found no significant differences in lamellar sensory surface area among lamellar positions. Previously, differences in lamellar sensory morphology have been tied to the internal fluid dynamics of the olfactory chamber. It is possible that the internal flow dynamics of these species are like other chondrichthyan models, where water flow patterns differ in the lateral vs the medial part of the organ, and the consistent distribution of sensory tissue does not correspond to this flow. Alternatively, the olfactory morphology of these species may result in uniform flow patterns throughout the olfactory chamber, correlating with the consistent distribution of sensory tissue throughout the organ. This study emphasizes that further investigations into chondrichthyan fluid dynamics is paramount to any future studies on the correlations between distribution of sensory tissues and water flow.
Y-shaped graft replacement of an isolated innominate artery aneurysm via the transmanubrial approach
Authors: Matsuzaki Kanji , Mitomi Kisato , Imai Akito , Sato Masataka 
Published: July 2024

Abstract
We report the rare case of an obese woman with a 32-mm isolated innominate artery aneurysm. Due to the deep location of the aneurysm within the thoracic inlet, we selected a transmanubrial osteomuscular sparing approach to obtain a clear field of vision. During the aneurysmectomy and reconstruction with a Y-shaped graft, we performed a temporary bypass using a contralateral leg of the graft to the right common carotid artery. Through these techniques, we successfully achieved complete resection and reconstruction of the aneurysm without the need for cardiopulmonary bypass.
Misinformation Detection in the Context of the Russian Invasion of Ukraine: Evidence From Original Survey Data Collected in 19 Democracies
Authors: Gehle Luisa , Hameleers Michael , Tulin Marina , de Vreese Claes , Aalberg Toril , Van Aelst Peter , S. Cardenal Ana , Corbu Nicoleta , van Erkel Patrick , Esser Frank , Halagiera Denis , Hopmann David , Koç-Michalska Karolina , Matthes Jörg , Meltzer Christine , Splendore Sergio , Stanyer James , Stępińska Agnieszka , Štětka Václav , Strömbäck Jesper , Terren Ludovic , Theocharis Yannis , Zoizner Alon 
Published: July 2024

Abstract
It can be difficult for citizens to discern factually accurate information from mis- and disinformation. Particularly in the context of the Russian invasion of Ukraine, the omnipresence of counterfactual narratives, propaganda, and partisan content may increase the likelihood that citizens select and accept mis -or disinformation. To assess citizens’ performance in discerning false statements from factually accurate information on the war, we utilized original survey data across 19 countries (N = 19,037) with soft quotas for age, education, and gender to approximate population characteristics. Our main findings indicate that people perform relatively well in discerning factually accurate information from false statements and that self-perceived media literacy and the need for cognition corresponds with better performance. Stronger pro-Russia views on the war correspond with a lower performance in misinformation detection, whereas anti-Russia attitudes are associated with better evaluations of the statements’ truthfulness. We find little cross-country variation in these patterns. As a key implication, we show that discernment between factually accurate information and mis- or disinformation is driven by both accuracy and partisan motivation and that these effects are similar across most different national contexts.
Misinformation as a Determinant of Response to COVID 19
Authors: Deo Shilpa , Mohanty Abhijit , Sharma Deependra , Sharma Sushil , Khisti Dinesh 
Published: July 2024

Abstract
Most of the micro and macro effects of the COVID-19 pandemic on the global economy have been investigated in the past two years. Few studies have examined COVID disinformation in non-Western countries. India produced the most social media disinformation, probably due to its high internet penetration, increased social media consumption, and low internet literacy (Al-Zaman, 2022a). To quantify the influence of disinformation on pandemic response, this study used mixed methods. The variables were examined through in-depth interviews. As they use digital media more than others, participants under 40 provided quantifiable data (The Future of India Foundation. (2022). Politics of disinformation: Why the current approaches are geared to fail and possible path forward. Retrieved from https://futureofindia.in/reports). It is mainly collected from metro cities of India. Moderation analysis using PLS-SEM examined whether self-perceived media literacy moderates the link between fake social media news and COVID-19 anxiety. The study findings have been linked to the theoretical foundation, the availability heuristic. This study holds significance as its implications will be beneficial in tackling the challenges associated with misinformation and its influence on response to pandemics that might be experienced in the future.
Sources of Human Immunodeficiency Virus Infections Among Men Who Have Sex With Men With a Migration Background: A Viral Phylogenetic Case Study in Amsterdam, The Netherlands
Authors: Blenkinsop Alexandra , Pantazis Nikos , Kostaki Evangelia Georgia , Sofocleous Lysandros , van Sighem Ard , Bezemer Daniela , van de Laar Thijs , van der Valk Marc , Reiss Peter , de Bree Godelieve , Ratmann Oliver 
Published: July 2024

Abstract

Background
Men and women with a migration background comprise an increasing proportion of incident human immunodeficiency virus (HIV) cases across Western Europe.

Methods
To characterize sources of transmission in local transmission chains, we used partial HIV consensus sequences with linked demographic and clinical data from the opt-out AIDS Therapy Evaluation in the Netherlands (ATHENA) cohort of people with HIV in the Netherlands and identified phylogenetically and epidemiologically possible HIV transmission pairs in Amsterdam. We interpreted these in the context of estimated infection dates, and quantified population-level sources of transmission to foreign-born and Dutch-born Amsterdam men who have sex with men (MSM) within Amsterdam transmission chains.

Results
We estimate that Dutch-born MSM were the predominant sources of infections among all Amsterdam MSM who acquired their infection locally in 2010–2021, and among almost all foreign-born Amsterdam MSM subpopulations. Stratifying by 2-year intervals indicated time trends in transmission dynamics, with a majority of infections originating from foreign-born MSM since 2016, although uncertainty ranges remained wide.

Conclusions
Native-born MSM have predominantly driven HIV transmissions in Amsterdam in 2010–2021. However, in the context of rapidly declining incidence in Amsterdam, the contribution from foreign-born MSM living in Amsterdam is increasing, with some evidence that most local transmissions have been from foreign-born Amsterdam MSM since 2016.
Comparison of 2 Serum Free Light Chain Assays with Creatinine Normal and Abnormal Populations Demonstrates the Need for Standardization
Authors: Griffiths Mark , Cheng Pow Lee , Wang Xiao Yan , Schneider Randal , Kulasingam Vathany 
Published: July 2024

Abstract

Background
The objective of this study was to compare The Binding Site’s Freelite on Optilite and Diazyme’s Kappa/Lambda free light chains (K/L FLC) on Abbott Architect c8000 with healthy and renal insufficient populations and to evaluate their respective reference intervals for serum free light chains (sFLCs).

Methods
Two hundred sixty serum samples were measured for creatinine and sFLCs by both assays and a subset by immunofixation electrophoresis. Verification of manufacturer-defined reference intervals was assessed.

Results
Kappa free light chains (KFLC) showed excellent correlation of 0.998 R2 with a slope of 0.73. For Lambda free light chains (LFLC), an acceptable correlation of 0.953 R2 was found with a slope of 1.50 as well as a skewness-based difference with a −12.70 intercept. Healthy estimated glomerular filtration rate (eGFR) ≥60 reference interval verification of central 95% could not be confirmed for either Freelite or Diazyme although LFLC was much closer than KFLC for both assays with Freelite KFLC recovering only 37% of values within reference interval claims. The K/L FLC ratio did not meet 100% claim for both Freelite (91%) and Diazyme (95%) among those with eGFR ≥60. Samples with eGFR ≤59 had increasingly higher levels of KFLC and LFLC for both assays. When comparing worsening eGFR status, Freelite recovered increasingly higher ratios while Diazyme recovered increasingly lower ratios.

Conclusions
Healthy reference intervals could not be verified for either Freelite or Diazyme. Renal reference intervals for Freelite are currently warranted while they are not recommended for Diazyme. The differences between these 2 assays can be minimized by standardization efforts such as recalibration.
Modulation of human gut microbiota by linear and branched fructooligosaccharides in anin vitrocolon model (TIM-2)
Authors: Popov Igor V , Koopmans Bram , Venema Koen 
Published: July 2024

AbstractAimsThis study aimed to compare the effects of linear and branched fructooligosaccharides (FOS) extracted from chicory and grass (Lolium perenne), respectively on human microbiota composition, diversity, and metabolism.Methods and resultsTo test the effects of linear and branched FOS on human microbiota we used the artificial in vitro human colon model (TIM-2). Microbiota composition and diversity were assessed by V3–V4 16S rRNA metagenomic sequencing, followed by differential taxa abundance and alpha/beta diversity analyses. SCFA/BCFA production was evaluated by gas chromatography–mass spectrometry. As a result, branched FOS had the most beneficial effects on microbial diversity and metabolite production. Also, branched FOS significantly increased the abundance of commensal bacteria associated with maintaining healthy gut functions and controlling inflammation, such as Butyricicoccus, Erysipelotrichaceae, Phascolarctobacterium, and Sutterella. Linear FOS also significantly increased the abundance of some other commensal gut bacteria (Anaerobutyricum, Lachnospiraceae, Faecalibacterium), but there were no differences in diversity metrics compared to the control.ConclusionsThe study revealed that branched FOS had the most beneficial effects compared to the linear FOS in vitro, concerning microbiota modulation, and metabolite production, making this a good candidate for further studies in food biotechnology.
Fabricated Legality
Authors: Parsi Lavinia 
Published: July 2024

Abstract
In the wake of the increased regulation of armed conflicts, legal interpretation by government lawyers has become crucial in shaping state policies, particularly during times of war. This article explores the scenarios where state policies that have been positively sanctioned by legal professionals potentially amount to international crimes, raising questions of accountability. By examining historical and contemporary instances, the article explores if and to what extent government legal advisers may be considered accomplices in such crimes. It suggests that their responsibility could be framed within the mode of liability of aiding and abetting, as outlined in Article 25(3)(c) of the Rome Statute. Additionally, the article addresses alternative modes of liability specified under Article 25(3)(a) and 25(3)(d). It finally expands to the further consequences of legal advice on the liability of the principal perpetrator, questioning whether the latter may invoke a defence of mistake of law or a breach of the principle of legality. In an attempt to embrace the revolutionary potential of international criminal law, this article aims to make legal interpretation itself the object of scrutiny, through the lenses of international criminal responsibility.
Effect on neutrophil migration and antimicrobial functions by the Bruton's tyrosine kinase inhibitors tolebrutinib, evobrutinib, and fenebrutinib
Authors: De Bondt Mirre , Renders Janne , Petit de Prado Paloma , Berghmans Nele , Pörtner Noëmie , Vanbrabant Lotte , de Oliveira Vívian Louise Soares , Duran Gayel , Baeten Paulien , Broux Bieke , Gouwy Mieke , Matthys Patrick , Hellings Niels , Struyf Sofie 
Published: July 2024

Abstract
Multiple sclerosis (MS) is a neurodegenerative, autoimmune disease that is still incurable. Nowadays, a variety of new drugs are being developed to prevent excessive inflammation and halt neurodegeneration. Among these are the inhibitors of Bruton's tyrosine kinase (BTK). Being indispensable for B cells, this enzyme became an appealing therapeutic target for autoimmune diseases. Recognizing the emerging importance of BTK in myeloid cells, we investigated the impact of upcoming BTK inhibitors on neutrophil functions. Although adaptive immunity in MS has been thoroughly studied, unanswered questions about the pathogenesis can be addressed by studying the effects of candidate MS drugs on innate immune cells such as neutrophils, previously overlooked in MS. In this study, we used 3 BTK inhibitors (evobrutinib, fenebrutinib, and tolebrutinib), and found that they reduce neutrophil activation by the bacterial peptide fMLF and the chemokine interleukin-8/CXCL8. Furthermore, they diminished the production of reactive oxygen species and release of neutrophil extracellular traps. Additionally, the production of CXCL8 and interleukin-1β in response to inflammatory stimuli was decreased. Inhibitory effects of the drugs on neutrophil activation were not related to toxicity. Instead, BTK inhibitors prolonged neutrophil survival in an inflammatory environment. Finally, treatment with BTK inhibitors decreased neutrophil migration toward CXCL8 in a Boyden chamber assay but not in a transendothelial setup. Also, in vivo CXCL1-induced migration was unaffected by BTK inhibitors. Collectively, this study provides novel insights into the impact of BTK inhibitors on neutrophil functions, thereby holding important implications for autoimmune or hematological diseases in which BTK is crucial.
From powerful knowledge to capabilities: social realism, social justice, and the Capabilities Approach
Authors: Talbot Daniel 
Published: July 2024

Abstract
This article argues that, as applied to education, the Capabilities Approach pioneered by Amartya Sen and Martha Nussbaum shares a range of philosophical commitments with the work of social realist scholars on the concept of ‘powerful knowledge’. I first trace the history of the concept of powerful knowledge and present critiques put forward by social justice scholars. I then outline the Capabilities Approach, arguing it provides a response to some of these concerns. From here I develop the connection between the two educational paradigms focusing on four areas of affinity. The first is the conceptual similarity between the ‘power’ of powerful knowledge and Nussbaum’s account of a capability. The second is their concern with the reduction of education to technical-instrumentalism, human capital, and other economically driven imperatives. The third is a shared focus on the link between knowledge, human agency, and freedom. The fourth, is a mutual rejection of relativism and embrace of a nuanced universalism. Ultimately, by drawing together these two conceptually rich approaches to the aims and purposes of education, I hope to open a space for theorizing about both capabilities and powerful knowledge that can resolve some of the problems in both. For powerful knowledge, the Capabilities Approach allows a more concrete explication of why access to disciplinary bodies of knowledge is of value to both individuals and society. On the side of the Capabilities Approach, engaging with the concept of powerful knowledge can help give a rationale for how educational knowledge fosters certain centrally important capabilities.
Withholding the letter: Transgender asylum seekers, legal gender recognition, and the UNHCR mandate
Authors: Camminga B 
Published: July 2024

Abstract
In the absence of legal gender recognition (LGR), transgender people carry documents that misrecognize them. The link between LGR’s absence and exposure to violence and exclusion—experiences that often force transgender people to flee—is clarified by scholarship. However, when a trans person seeks asylum, they are often provided with documents that, rather than unambiguously recognizing their bearer, repeat this misrecognition. This incongruence often exposes transgender asylum seekers to greater risk. Previously, I have argued that, at the moment in which they seek international protection, transgender asylum seekers experience a form of statelessness due to the absence of LGR. In this paper, I build on this argument, drawing on a range of empirical studies to suggest that conditions of statelessness already exist prior to fleeing. As a result, I argue that the UNHCR is under obligation to provide documents recognizing their holder, even if this runs counter to state legislation.
Genetically clustered antifungal phytocytokines and receptor protein family members cooperate to trigger plant immune signaling
Authors: Lintz Julie , Goto Yukihisa , Bender Kyle W , Bchini Raphaël , Dubrulle Guillaume , Cawston Euan , Zipfel Cyril , Duplessis Sebastien , Petre Benjamin 
Published: July 2024

Abstract
Phytocytokines regulate plant immunity by cooperating with cell surface proteins. Populus trichocarpa RUST INDUCED SECRETED PEPTIDE 1 (PtRISP1) exhibits an elicitor activity in poplar, as well as a direct antimicrobial activity against rust fungi. The PtRISP1 gene directly clusters with a gene encoding a leucine-rich repeat receptor protein (LRR-RP), that we termed RISP-ASSOCIATED LRR-RP (PtRALR). In this study, we used phylogenomics to characterize the RISP and RALR gene families, and molecular physiology assays to functionally characterize RISP/RALR pairs. Both RISP and RALR gene families specifically evolved in Salicaceae species (poplar and willow), and systematically cluster in the genomes. Despite a low sequence identity, Salix purpurea RISP1 (SpRISP1) shows properties and activities similar to PtRISP1. Both PtRISP1 and SpRISP1 induced a reactive oxygen species (ROS) burst and phosphorylation of mitogen-activated protein kinases (MAPKs) in Nicotiana benthamiana leaves expressing the respective clustered RALR. PtRISP1 also triggers a rapid stomatal closure in poplar. Altogether, these results indicate that plants evolved phytocytokines with direct antimicrobial activities, and that the genes encoding these phytocytokines co-evolved and physically cluster with genes encoding LRR-RPs required to initiate immune signaling.