bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Phlaeothrips-lineage of fungus feeding thrips (Thysanoptera: Phlaeothripidae) in Iran with a new species of Hindsiothrips
Authors: MINAEI KAMBIZ 
Published: January 2013

Hindsiothrips sisakhti sp. n. is described from leaf litter in Iran, this being the first record of the genus from this country. A key is provided to the seven Phlaeothripinae genera recorded from Iran that are considered members of the Phlaeothrips-lineage, in which most species are fungus feeding: Aleurodothrips, Hindsiothrips, Hoplandrothrips, Hoplothrips, Idiothrips, Phlaeothrips and Stictothrips. Structural variation in the group is discussed briefly, and Idiothrips ficus Bhatti is considered a new synonym of Idiothrips bellus Faure.
Two new species of the genus Anoplocheylus Berlese, 1910 (Acari: Trombidiformes: Pseudocheylidae) from Iran
Authors: BAGHERI MOHAMMAD , ZAREI ELHAM , AHANIAZAD MANSOUREH , GHAREKHANY GHOLAMHOSSEIN , NAVAEI-BONAB REZA 
Published: January 2013

Two new species of the genus Anoplocheylus Berlese, 1910 (Acari: Trombidiformes: Pseudocheylidae), A. sinai Bagheri sp. nov. and A. kazemii Bagheri sp. nov., are described and figured. An updated key to all known species of Anoplocheylus is also provided.
First record of Trichosteresis Förster (Hymenoptera: Megaspilidae) from South America
Authors: MACEDO ANTONIO CARLOS CRUZ 
Published: January 2013

The family Megaspilidae worldwide comprises more than 450 species in 11 genera (Dessart 2006), but the fauna of the family in South America is poorly known. For example, Dendrocerus Ratzeburg has been the only named genus reported from either Argentina (Loiácono 1998; Martinez 2003) or Brazil (Loiácono & Margaría 2002).
A new genus and species of Bruchinae, with a key to the genera from Australia (Coleoptera: Chrysomelidae)
Authors: REID Chris A. M. , BEATSON M. 
Published: January 2013

A new genus of Bruchinae, Buburra Reid & Beatson, is erected for a single new species, B. jeanae Reid & Beatson. Buburra is endemic to Australia where it is known from a single site at high altitude in Victoria. The hostplant is unknown. Buburra is placed in the tribe Pachymerini. A key is provided for identification of the 12 genera of Bruchinae in Australia, including Caryotrypes Decelle, 1968, recorded from Australia for the first time. Five Australian species described in Bruchus Linneus, 1767, are newly transferred to Bruchidius Schilsky, 1905: Bruchidius diversipes (Lea, 1899) comb. nov.; B. maestus (Lea, 1899) comb. nov.; B. oodnadattae (Blackburn, 1900) comb. nov.; B. persimulans (Blackburn, 1900) comb. nov.; B. quornensis (Blackburn, 1900) comb. nov. A checklist is provided for the species of Bruchinae in Australia.
The activation peptide cleft exposed by thrombin cleavage of FXIII-A2 contains a recognition site for the fibrinogen α chain
Authors: Smith Kerrie A. , Pease Richard J. , Avery Craig A. , Brown Jane M. , Adamson Penelope J. , Cooke Esther J. , Neergaard-Petersen Søs , Cordell Paul A. , Ariëns Robert A. S. , Fishwick Colin W. G. , Philippou Helen , Grant Peter J. 
Published: January 2013

Key PointsFibrin αC389-402 binds a cleft in the β-sandwich domain of FXIII-A2* exposed only after cleavage of the activation peptide. Binding of fibrin αC389-402 to FXIII-A2* regulates fibrin cross-linking and thus clot stabilization and fibrinolysis.
The COP1 E3-ligase interacts with FIP200, a key regulator of mammalian autophagy
Authors: Kobayashi Saori , Yoneda-Kato Noriko , Itahara Nagisa , Yoshida Akihiro , Kato Jun-ya 
Published: January 2013

Abstract

Background
The ubiquitin ligase COP1, COnstitutively Photomorphogenic 1, functions in many biological responses in mammalian cells, but its downstream pathway remains unclear.

Results
Here, we identified FIP200, a key regulator of mammalian autophagy, as a novel COP1-interacting protein by yeast two-hybrid screening. The interaction was confirmed by a GST-pulldown assay. Split-GFP analysis revealed that interaction between COP1 and FIP200 predominantly occurred in the cytoplasm and was enhanced in cells treated with UV irradiation. Different forms of FIP200 protein were expressed in cultured mammalian cells, and ectopic expression of COP1 reduced one of such forms.

Conclusions
These data suggest that COP1 modulates FIP200-associated activities, which may contribute to a variety of cellular functions that COP1 is involved in.
Consistent mutational paths predict eukaryotic thermostability
Authors: van Noort Vera , Bradatsch Bettina , Arumugam Manimozhiyan , Amlacher Stefan , Bange Gert , Creevey Chris , Falk Sebastian , Mende Daniel R , Sinning Irmgard , Hurt Ed , Bork Peer 
Published: January 2013

Abstract

Background
Proteomes of thermophilic prokaryotes have been instrumental in structural biology and successfully exploited in biotechnology, however many proteins required for eukaryotic cell function are absent from bacteria or archaea. With Chaetomium thermophilum, Thielavia terrestris and Thielavia heterothallica three genome sequences of thermophilic eukaryotes have been published.

Results
Studying the genomes and proteomes of these thermophilic fungi, we found common strategies of thermal adaptation across the different kingdoms of Life, including amino acid biases and a reduced genome size. A phylogenetics-guided comparison of thermophilic proteomes with those of other, mesophilic Sordariomycetes revealed consistent amino acid substitutions associated to thermophily that were also present in an independent lineage of thermophilic fungi. The most consistent pattern is the substitution of lysine by arginine, which we could find in almost all lineages but has not been extensively used in protein stability engineering. By exploiting mutational paths towards the thermophiles, we could predict particular amino acid residues in individual proteins that contribute to thermostability and validated some of them experimentally. By determining the three-dimensional structure of an exemplar protein from C. thermophilum (Arx1), we could also characterise the molecular consequences of some of these mutations.

Conclusions
The comparative analysis of these three genomes not only enhances our understanding of the evolution of thermophily, but also provides new ways to engineer protein stability.
Cartilage collagen damage in hip osteoarthritis similar to that seen in knee osteoarthritis; a case–control study of relationship between collagen, glycosaminoglycan and cartilage swelling
Authors: Hosseininia Shahrzad , Lindberg Lisbeth R , Dahlberg Leif E 
Published: January 2013

Abstract

Background
It remains to be shown whether OA shares molecular similarities between different joints in humans. This study provides evidence for similarities in cartilage molecular damage in osteoarthritic (OA) joints.

Methods
Articular cartilage from osteoarthritic hip joints were analysed and compared to non-OA controls regarding collagen, glycosaminoglycan and water content. Femoral heads from 16 osteoarthritic (OA) and 20 reference patients were obtained from hip replacement surgery due to OA and femoral neck fracture, respectively. Cartilage histological changes were assessed by Mankin grading and denatured collagen type II immunostaining and cartilage was extracted by α-chymotrypsin. Hydroxyproline and Alcian blue binding assays were used to measure collagen and glycosaminoglycan (GAG) content, respectively.

Results
Mankin and immunohistology scores were significantly higher in hip OA samples than in reference samples. Cartilage water content was 6% higher in OA samples than in references. 2.5 times more collagen was extracted from OA than from reference samples. There was a positive association between water content and percentage of extractable collagen pool (ECP) in both groups. The amounts of collagen per wet and dry weights did not differ statistically between OA and reference cartilage. % Extractable collagen was not related to collagen per dry weight in either group. However when collagen was expressed by wet weight there was a negative correlation between % extractable and collagen in OA cartilage. The amount of GAG per wet weight was similar in both groups but the amount of GAG per dry weight was higher in OA samples compared to reference samples, which suggests a capacity for GAG biosynthesis in hip OA cartilage. Neither of the studied parameters was related to age in either group.

Conclusions
Increased collagen extractability and water content in human hip cartilage is associated with OA pathology and can be observed at early stages of the degenerative hip OA process. Our results suggest a common degradative pathway of collagen in articular cartilage of different joints. Furthermore, the study suggests that biochemical changes precede more overt OA changes and that chondrocytes may have a capability to compensate molecular loss in the early phase of OA.
In vitro antioxidant and antiproliferative activities of plants of the ethnopharmacopeia from northwest of Mexico
Authors: Jiménez-Estrada Manuel , Velázquez-Contreras Carlos , Garibay-Escobar Adriana , Sierras-Canchola Davisela , Lapizco-Vázquez Ricardo , Ortiz-Sandoval Carolina , Burgos-Hernández Armando , Robles-Zepeda Ramón Enrique 
Published: January 2013

Abstract

Background
The aim of this study, is to investigate the in vitro antioxidant activity, the total phenols content, the flavonoids content and the antiproliferative activity of methanolic extracts of the plants: Krameria erecta, Struthanthus palmeri, Phoradendron californicum, Senna covesii and Stegnosperma halimifolium, used by different ethnic groups from northwestern Mexico in the treatment and cure of various diseases.

Methods
The in vitro antioxidant activity was measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing/Antioxidant Power assay (FRAP), the total phenols content was measured by Folin–Ciocalteau assay, the flavonoids content by the AlCl3 colorimetric method and the antiproliferative activity (line cells HeLa, RAW 264.7, M12Ak.C3.F6 and L929) using MTT method.

Results
The K. erecta extract showed the higher radical scavenging activity (67.88%), antioxidant activity by FRAP (1.41 mg Trolox Eq), the highest total phenols content (598.51 mg Galic Acid Eq/g extract), the highest flavonoids content (3.80 mg Quercetin Eq/g extract) and the greatest antiproliferative activity in a dose dependent manner against most Cell line evaluated. A positive correlation was found between the antioxidant activity and the flavonoids content.

Conclusions
This study is the first report on the antioxidant and antiproliferative activities of the five species evaluated. The results demostrate that there is a positive correlation between antioxidant activity and the flavonoids content, indicating that these type of polyphenols could be the major contributors to the observed antioxidant activity in the evaluated plant extracts. Of the extracts evaluated, that of Krameria erecta showed the greatest antioxidant and antiproliferative activities, a discovery that makes this species a promising candidate for future research.
Implementation of geriatric assessment and decision support in residential care homes: facilitating and impeding factors during initial and maintenance phase
Authors: Boorsma Marijke , Langedijk Eveliene , Frijters Dinnus HM , Nijpels Giel , Elfring Tom , van Hout Hein PJ 
Published: January 2013

Abstract

Background
Successfully introducing and maintaining care innovations may depend on the interplay between care setting, the intervention and specific circumstances. We studied the factors influencing the introduction and maintenance of a Multidisciplinary Integrated Care model in 10 Dutch residential care homes.

Methods
Facilitating and impeding factors were studied and compared at the time of introduction of the interRAI-LTCF assessment method in residential care homes as well as three years later, by surveys and semi structured interviews among nurse staff, managers, and physicians.

Results
Facilitating factors at introduction were positive opinions of staff and family physicians about the changes of the process of care and the anticipated improvement of quality of care. Staff was positive about the applicability of the software to support the interRAI-LTCF assessments. Impeding factors were time constraints to complete interRAI-LTCF assessments and insufficient computer equipment.
In the maintenance phase, the positive attitude of the location manager and the perceived benefits of the care model and the interRAI-LTCF assessment method were most important. Impeding factors after 3 years remained the lack of time to complete the assessments and lack of sufficient computer equipment.

Conclusions
Impeding and facilitating factors were comparable in the initial and maintenance phase. Adoption of the interRAI-LTCF assessment method depended on positive opinions of staff and management, continuing support of staff and the availability of sufficient computer equipment.
Coronary aspirate TNFα reflects saphenous vein bypass graft restenosis risk in diabetic patients
Authors: Baars Theodor , Konorza Thomas , Kahlert Philipp , Möhlenkamp Stefan , Erbel Raimund , Heusch Gerd , Kleinbongard Petra 
Published: January 2013

Abstract

Background
Patients with diabetes mellitus (DM) have an increased risk for periprocedural complications and adverse cardiac events after percutaneous coronary intervention. We addressed the potential for coronary microvascular obstruction and restenosis in patients with and without DM undergoing stenting for saphenous vein bypass graft (SVG) stenosis under protection with a distal occlusion/aspiration device.

Methods
SVG plaque volume and composition were analyzed using intravascular ultrasound before stent implantation. Percent diameter stenosis was determined from quantitative coronary angiography before, immediately after and 6 months after stent implantation. Coronary aspirate was retrieved during stent implantation and divided into particulate debris and plasma. Total calcium, several vasoconstrictors, and tumor necrosis factor (TNF)α in particulate debris and coronary aspirate plasma were determined.

Results
Patients with and without DM had similar plaque volume, but larger necrotic core and greater particulate debris release in patients with than without DM (20.3±2.7 vs. 12.7±2.6% and 143.9±19.3 vs. 75.1±10.4 mg, P<0.05). The TNFα concentration in particulate debris and coronary aspirate plasma was higher in patients with than without DM (15.9±6.6 vs. 5.1±2.4 pmol/mg and 2.2±0.7 vs. 1.1±0.2 pmol/L, P<0.05), whereas total calcium and vasoconstrictors were not different. Patients with DM had a greater percent diameter stenosis 6 months after stent implantation than those without DM (22.17±5.22 vs. 6.34±1.11%, P<0.05). The increase in TNFα immediately after stent implantation correlated with restenosis 6 months later (r=0.69, P<0.05).

Conclusion
In diabetics, particulate debris and coronary aspirate plasma contained more TNFα, which might reflect the activity of the underlying atherosclerotic process.

Trial registration
URL: http://www.clinicaltrials.gov/ct2/results?term=NCT01430884; unique identifier: NCT01430884
Bariatric surgery: the challenges with candidate selection, individualizing treatment and clinical outcomes
Authors: Neff KJ , Olbers T , le Roux CW 
Published: January 2013

Abstract
Obesity is recognized as a global health crisis. Bariatric surgery offers a treatment that can reduce weight, induce remission of obesity-related diseases, and improve the quality of life. In this article, we outline the different options in bariatric surgery and summarize the recommendations for selecting and assessing potential candidates before proceeding to surgery. We present current data on post-surgical outcomes and evaluate the psychosocial and economic effects of bariatric surgery. Finally, we evaluate the complication rates and present recommendations for post-operative care.
A novel data mining system points out hidden relationships between immunological markers in multiple sclerosis
Authors: Gironi Maira , Saresella Marina , Rovaris Marco , Vaghi Matilde , Nemni Raffaello , Clerici Mario , Grossi Enzo 
Published: January 2013

AbstractBackgroundMultiple Sclerosis (MS) is a multi-factorial disease, where a single biomarker unlikely can provide comprehensive information. Moreover, due to the non-linearity of biomarkers, traditional statistic is both unsuitable and underpowered to dissect their relationship. Patients affected with primary (PP=14), secondary (SP=33), benign (BB=26), relapsing-remitting (RR=30) MS, and 42 sex and age matched healthy controls were studied. We performed a depth immune-phenotypic and functional analysis of peripheral blood mononuclear cell (PBMCs) by flow-cytometry. Semantic connectivity maps (AutoCM) were applied to find the natural associations among immunological markers. AutoCM is a special kind of Artificial Neural Network able to find consistent trends and associations among variables. The matrix of connections, visualized through minimum spanning tree, keeps non linear associations among variables and captures connection schemes among clusters.ResultsComplex immunological relationships were shown to be related to different disease courses. Low CD4IL25+ cells level was strongly related (link strength, ls=0.81) to SP MS. This phenotype was also associated to high CD4ROR+ cells levels (ls=0.56). BB MS was related to high CD4+IL13 cell levels (ls=0.90), as well as to high CD14+IL6 cells percentage (ls=0.80). RR MS was strongly (ls=0.87) related to CD4+IL25 high cell levels, as well indirectly to high percentages of CD4+IL13 cells. In this latter strong (ls=0.92) association could be confirmed the induction activity of the former cells (CD4+IL25) on the latter (CD4+IL13). Another interesting topographic data was the isolation of Th9 cells (CD4IL9) from the main part of the immunological network related to MS, suggesting a possible secondary role of this new described cell phenotype in MS disease.ConclusionsThis novel application of non-linear mathematical techniques suggests peculiar immunological signatures for different MS phenotypes. Notably, the immune-network displayed by this new method, rather than a single marker, might be viewed as the right target of immunotherapy. Furthermore, this new statistical technique could be also employed to increase the knowledge of other age-related multifactorial disease in which complex immunological networks play a substantial role.
Two types of mental fatigue affect spontaneous oscillatory brain activities in different ways
Authors: Shigihara Yoshihito , Tanaka Masaaki , Ishii Akira , Kanai Etsuko , Funakura Masami , Watanabe Yasuyoshi 
Published: January 2013

Abstract

Background
Fatigue has a multi-factorial nature. We examined the effects of two types of mental fatigue on spontaneous oscillatory brain activity using magnetoencephalography (MEG).

Methods
Participants were randomly assigned to two groups in a single-blinded, crossover fashion to perform two types of mental fatigue-inducing experiments. Each experiment consisted of a 30-min fatigue-inducing 0- or 2-back test session and two evaluation sessions performed just before and after the fatigue-inducing mental task session.

Results
After the 0-back test, decreased alpha power was indicated in the right angular gyrus and increased levels in the left middle and superior temporal gyrus, left postcentral gyrus, right superior frontal gyrus, left inferior frontal gyrus, and right medial frontal gyrus. After the 2-back test, decreased alpha power was indicated in the right middle and superior frontal gyrus and increased levels in the left inferior parietal and superior parietal lobules, right parahippocampal gyrus, right uncus, left postcentral gyrus, left middle frontal gyrus, and right inferior frontal gyrus. For beta power, increased power following the 0-back test was indicated in the left middle temporal gyrus, left superior frontal gyrus, left cingulate gyrus, and left precentral gyrus. After the 2-back test, decreased power was suggested in the left superior frontal gyrus and increased levels in the left middle temporal gyrus and left inferior parietal lobule. Some of these brain regions might be associated with task performance during the fatigue-inducing trials.

Conclusions
Two types of mental fatigue may produce different alterations of the spontaneous oscillatory MEG activities. Our findings would provide new perspectives on the neural mechanisms underlying mental fatigue.
Synchronous plexiform neurofibroma in the arytenoids and neurofibroma in the parapharynx in a patient with non-neurofibromatosis: a case report
Authors: Son Hee Young , Shim Hyun Seok , Kim Jin Pyeong , Woo Seung Hoon 
Published: January 2013

Abstract

Introduction
Plexiform neurofibroma of the larynx is a rare disease. In this report, we present a plexiform neurofibroma in the arytenoids and neurofibroma in the parapharynx detected coincidently.

Case presentation
A 56-year-old Asian woman presented with respiratory distress and episodes of apnea at night. A solitary mass from the left arytenoids was found to be nearly obstructing the airway and causing the sleep apnea. There was also a parapharynx mass protruding into the pharynx. The parapharynx tumor was removed with the lateral incision approach, and the arytenoid tumor was removed with a transoral carbon dioxide laser. The pathologic diagnosis was plexiform neurofibroma for the arytenoid mass and neurofibroma for the parapharynx mass.

Conclusion
We have reported an extremely rare case of plexiform neurofibroma in the arytenoids and neurofibroma in the parapharynx. This entity may be considered in the differential diagnosis of all laryngeal and parapharynx masses.
Metabolic syndrome and menopause
Authors: Jouyandeh Zahra , Nayebzadeh Farnaz , Qorbani Mostafa , Asadi Mojgan 
Published: January 2013

Abstract
Background
The metabolic syndrome is defined as an assemblage of risk factors for cardiovascular diseases, and menopause is associated with an increase in metabolic syndrome prevalence. The aim of this study was to assess the prevalence of metabolic syndrome and its components among postmenopausal women in Tehran, Iran.

Methods
In this cross-sectional study in menopause clinic in Tehran, 118 postmenopausal women were investigated. We used the adult treatment panel 3 (ATP3) criteria to classify subjects as having metabolic syndrome.

Results
Total prevalence of metabolic syndrome among our subjects was 30.1%. Waist circumference, HDL-cholesterol, fasting blood glucose, diastolic blood pressure ,Systolic blood pressure, and triglyceride were significantly higher among women with metabolic syndrome (P-value<0.05). Our study shows high abdominal obesity and hypertension are the most prevalent components of metabolic syndrome. 15%, 13.3% and 1.8% of subjects had three, four and five criteria for metabolic syndrome, respectively. There was a significant relationship between number of components of metabolic syndrome and waist circumference.

Conclusions
Our study shows that postmenopausal status is associated with an increased risk of metabolic syndrome. Therefore, to prevent cardiovascular disease there is a need to evaluate metabolic syndrome and its components from the time of the menopause.