bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANALYSIS OF THE LIPIDS FROM THE CONTENTS OF AN EPIDERMAL CYST
Authors: Otsuka Fujio , Kukita Atsushi , Seyama Yousuke , Yamakawa Tamio 
Published: April 2015

ABSTRACTThe lipids from the contents of an epidermal cyst were analysed quantitatively. Cholesteryl ester, triglyceride, free fatty acids, cholesterol, ceramide and some phospholipids were determined.Fatty acid components of some lipids (cholesteryl ester, triglyceride, free fatty acids and ceramide) were also analysed. The fatty acid component of ceramide included a high proportion of long chain fatty acids. The fatty acid portion of cholesteryl ester contained a relatively larger proportion of odd numbered, straight chained monoenes (C17:1 etc), which were also found in ceramide at a lower concentration. The odd numbered monoene, C17:1 in which the double bond is located at Δ9, was considered to be an intrinsic component of epidermal cyst contents.The lipid pattern and fatty acid compositions of the lipids of the contents from an epidermal cyst were similar to those of the human epidermal corneum.
TICK BITES: A STUDY USING SCANNING ELECTRON MICROSCOPY
Authors: Aoki Kiyoko , Mori Makusu , Kamata Hideaki , Suzuki Hiroyuki 
Published: April 2015

ABSTRACTA 14‐year‐old girl was visiting Karuizawa when a tick attached itself to her right upper chest. A purple‐red lesion was induced near the base of the neck. In the center of it, small black particles, the remains of the tick, were present. The tick was identified as an adult female Ixodes ovatus.Light microscopy: In the dermis near the area where the tick had attached itself, perivascular and periadnexal cellular infiltrations were observed, mainly lymphocytes and neutrophils.Scanning electron microscopy: Parts of the capitulum could be observed on the surface of the skin. The hypostome with its sharp denticles had been inserted from above through the epidermis into the dermis. A cement‐like substance, suggesting a secretion, was observed on the dorsal hypostome. An intact Ixodes ovatus was also examined by scanning electron microscopy for comparison.The present study has probably produced the very first clear visual demonstration of the tick's secretion flow pattern and effect on the surrounding tissue.
CONJUGATION OF SOME PHENOLIC COMPOUNDS WITH AMINO GROUPS AND RELATED SENSITIZATION POTENCY
Authors: Osamura Hiromi , Takeuchi Yoshio , Urayama Tsutomu , Itoh Masatoshi , Ishihara Masaru 
Published: April 2015

AbstractThe conjugation of some phenolic compounds with ten dansyl amino acids was examined and the results indicated the importance of conjugation with the lysine residue.The conjugation of phenolic compounds with monodansyl cadaverine and cadaverine was also studied. The results obtained by these in vitro tests correlated well with those of animal sensitization tests and patch tests on patients with contact dermatitis.
BIOCHEMICAL CHANGES AFTER THE ORAL ADMINISTRATION OF RETINOID IN THE HORNY LAYER OF PATIENTS WITH KERATINIZATION DISORDERS
Authors: Yoshiike Takashi , Negi Makoto , Manabe Motomu , Takamori Kenji , Ogawa Hideoki 
Published: April 2015

AbstractFour cases with keratinization disorders were treated with aromatic retinoid (Ro 10‐9359), with resulting clinical improvement. Horny layers from these patients were obtained before and after treatment, and fractionated into keratin fibers, soluble proteins, and membraneous fractions. These were studied using SDS gel electrophoresis and amino‐acid analysis, and a comparison was made between the findings before and after retinoid administration. Membraneous fractions from diseased horny layers contained abnormal amino acid compositions, in particular, reduced half‐cystine and proline residues, which tended to become normalized after the treatment with retinoid. Normalization of keratin fibers and soluble fractions was not as obvious on SDS gel electrophoresis.
CELL KINETIC STUDIES OF THE EFFECT OF DTIC COMBINED WITH FLUORESCENT LIGHT OR UVA IRRADIATION ON HUMAN MELANOMA CELL LINE (SEKI, II)
Authors: Shimada Shinji , Kawashima Makoto , Watanabe Shinichi , Yamada Kiyoshi , Mizoguchi Masako , Hori Yoshiaki , Kukita Atsushi 
Published: April 2015

AbstractThe effectiveness of DTIC (5‐(3.3‐dimethyl‐1‐triazeno) imidazole‐4‐carboxamide) against a human malignant melanoma cell line (SEKI, II) was investigated with the additional use of fluorescent light and long wave ultraviolet irradiation. The cell cycle phase distribution of cultured melanoma cells exposed to DTIC (10 μg/ml, 100 μg/ml) was analyzed by means of flow‐cytometry (ICP type 11). The cytostatic effect of DTIC was observed at a concentration higher than 10 μg/ml in standard culture. Remarkable enhancement of the effect of DTIC was observed in both fluorescent light and ultraviolet irradiation. The most prominent change in cell cycle phase occurred in the group treated with DTIC 100 μg/ml with fluorescent light. S phase prolongation was shown in early stages (6, 12 hours), and then G2M phase accumulation was observed in late stages (48 hours). In ultraviolet irradiation groups, enhancement of the cytocidal effect of DTIC was observed 12 hours after irradiation. The death of all of the cells was observed in the DTIC 10 μg/ml + UVA 3 joules/cm2 and the DTIC 100 μg/ml + UVA 1 joule/cm2 groups. DTIC with UVA irradiation may be used for the treatment of cutaneous malignant tumors.
CHARACTERIZATION OF BASAL CELL EPITHELIOMA IN THE JAPANESE
Authors: Ono Tomomichi , Kitoh Masato , Kuriya Norikazu 
Published: April 2015

AbstractClinicopathological and statistical studies were made on one hundred and fifty‐six Japanese cases of basal cell epithelioma. A new formula was presented for the calculation of the growth rate of BCE, as follows,Size of tumor (major axis × minor axis) (mm2) = 1.7363 × T2 + 133.1192T = Years which had elapsed from the presumed time of onset to the time of the first examinationThe yearly growth in diameter calculated from the above formula is less than 1.0 mm within ten years after onset, and 1.0 mm to 1.3 mm thereafter. The time needed for BCE to double its size averaged 8.8 years in the present study.In addition to the slow rate of progression, BCE in the Japanese was characterized by its pigmentation; 84% were either partially or totally pigmented, giving the BCE the so‐called black and pearly appearance.
ADENOSINE DEAMINASE IN ADULT T CELL LEUKEMIA
Authors: Iizuka Hajime , Ohkawara Akira , Koizumi Hiroko , Aoyagi Takashi , Miura Yusho , Taniguchi Naoyuki , Wakisaka Akemi 
Published: April 2015

ABSTRACTIncreased adenosine deaminase activity was observed in peripheral lymphocytes from a patient with adult T cell leukemia. Gel filtration pattern on a Sephadex G‐150 revealed that the enzyme from normal lymphocytes existed in two forms with different molecular weights (large and small), whereas the enzyme from the patient existed solely as the small molecular weight form. Comparison of this small form adenosine deaminase from the patient with that from normal controls revealed that both enzymes were quite similar in relative substrate specificity, Km values for adenosine, pH optima, heat stability pattern, and sensitivity to inhibition by coformycin, a tight binding inhibitor of adenosine deaminase. Increased adenosine deaminase activity and the lack of the large form of the enzyme might be significant features of adult T cell leukemia lymphocytes.
LOCALIZATION AND POSSIBLE ACTIVATION MECHANISMS OF TRANSGLUTAMINASE IN THE SKIN
Authors: Negi Makoto 
Published: April 2015

ABSTRACTThe activity of transglutaminase per unit of wet weight, dry weight, soluble protein, and DNA was determined in three fractions (stratum corneum, malpighii, and dermis) of cow snout skin. The stratum corneum showed the highest enzyme activity among these three fractions.The activity of transglutaminase in the stratum malpighii was ehhanced remarkably by a 36 hour preincubation period at pH 4.5, but the activity in the stratum corneum did not show any increase in enzyme activity under acidic conditions. This ehhancement of enzyme activity was inhibited by pepstatin (100 μg/ml) and chymostatin (100 μg/ml).Relatively high activities of lysosomal enzymes such as β‐glucuronidase, cathepsin B1, and cathepsin D were detected in the stratum malpighii fraction, but these enzyme activities were low in the stratum corneum fraction.To further clarify the nature and activation systems of transglutaminase from stratum corneum and malpighii, transglutaminase solutions with the same activity were prepared from the two layers. Then the same amount of cathepsin D in the crude malpighian transglutaminase solution was added in the crude transglutaminase solution of stratum corneum. Following incubation, the transglutaminase activity of stratum corneum became elevated to the same level of enzyme activity as that of the stratum malpighii. These results suggest the importance of cathepsin D in the activation system of transglutaminase and possible activation machanisms of epidermal transglutaminase in vivo. Transglutaminase in the stratum malpighii may exist in a nonactivated form. During the autolytic stages in the stratum granulosum, a small amount of this nonactivated transglutaminase may be activated by released lysosomal acid proteinases such as cathepsin B1 and cathepsin D. Thereafter, activated transglutaminase could catalyze the formations of ε‐(γ‐glutamyl) lysine cross‐linking in the proteins synthesized in the cytosol of keratinizing cells with ultimate transformation to the membrane‐lining protein, the so‐called marginal band.In addition, most of the transglutaminase in the stratum corneum may still remain in a nonactivated form, since cathepsin D like enzymes may possibly be inactivated or degraded in the stratum corneum in vivo.
EXAMINATION OF SOME PROPERTIES OF DERMATOPHYTES FOR LONG‐TERM PRESERVATION USING PERIODIC TRANSFER METHOD
Authors: Kinbara Takeshi , Mashimo Masami , Fukushiro Ryoichi 
Published: April 2015

ABSTRACTSeventy‐two strains from species of dermatophytes which had been transferred periodically on Sabouraud's dextrose agar for more than ten years were examined for their morphology, physiological activities and biological properties.Of 42 strains of Trichophyton rubrum, 17 (40.4%) were positive for pigment production on Sabouraud's dextrose agar. In mating experiments, 15 (79%) of 19 strains tested were compatible with Arthroderma simii and proved to be of the “—” mating type. Eight (67%) of 12 strains in the Microsporum gypseum complex were positive for macroconidium formation. In mating experiments, 4 of 12 strains proved to be Nannizzia gypsea of the “+” type, 3 to be of the “—” type, and one to be N. incurvata of the “—” type.Most of the 18 strains in the T. mentagrophytes complex and M. canis have been maintained with alterations.
VARIOUS CELLULAR ACTIVITIES OF THE OUTGROWING EPIDERMIS IN PIG SKIN EXPLANT CULTURE—THE EFFECTS OF METABOLIC INHIBITORS
Authors: Aoyagi Takashi , Umeda Katsuko , Kato Naoko , Fukaya Toru , Miura Yusho 
Published: April 2015

ABSTRACTThe effects of metabolic inhibitors on the outgrowing epidermis were studied to detect the various cellular activities of epidermal cells. Hydroxyurea significantly inhibited mitosis and 3H‐thymidine uptake, but the inhibitory effect of hydroxyurea on the rate of epidermal outgrowth was less remarkable. On the other hand, cytochalasin B significantly inhibited the rate of epidermal outgrowth but did not inhibit mitosis or 3H‐thymidine uptake. The overall results indicate that there are several cellular activities such as migration and proliferation in the outgrowing epidermis in vitro.
NON‐HODGKIN'S LYMPHOMA SIMULATING ERYTHEMA NODOSUM
Authors: Sato Akihiko , Mikami Masatsugu , Konno Hidehiko , Usuba Makoto 
Published: April 2015

ABSTRACTTwo cases of malignant lymphoma whose initial clinical presentations simulated unilateral erythema nodosum on the leg are reported. In one case, the first diagnosis was in error, while, in the other, the diagnosis was confirmed by the histology. The patients later developed widespread malignant lymphoma. An increased awareness of this symptomology in patients with malignant lymphoma may lead to earlier diagnosis and appropriate therapy.
RAPID DIAGNOSIS OF HERPETIC DISEASE BY THE PARKER INK METHOD
Authors: Saruta Takao 
Published: April 2015

ABSTRACTIn place of the Giemsa method hitherto used in cytology for viral diseases, we applied a method using Parker Ink to herpes simplex, herpes zoster, and chicken pox infected tissues and found that this method is capable of detecting viral giant cells more easily and rapidly than the Giemsa method.Thus it was concluded that this method is useful in the rapid differential diagnosis of herpetic diseases otherwise difficult to diagnose clinically and can be used in the field of dermatology as easily as the caustic potash method is used as a fungal test.
IMMUNOFLUORESCENCE CHARACTERISTICS AND SOME BIOCHEMICAL PROPERTIES OF LARGE MOLECULAR WEIGHT KERATIN FROM HUMAN PLANTAR STRATUM CORNEUM
Authors: Hosokawa Michiko , Masu Shinichi 
Published: April 2015

ABSTRACTKeratin subunits of 64K, 53K, and 43K M.W. from human plantar stratum corneum fibrous proteins were isolated by SDS polyacrylamide gel electrophoresis (PAGE), and the antiserum against each keratin was prepared from rabbits. During indirect immunofluorescence studies of the normal skin, the whole epidermis stained positively with anti‐64K keratin antiserum (64K antiserum), but the positive staining of the basal layer diminished with the dilution of the antiserum. A similar staining pattern was observed with 53K antiserum, although the diminished staining of the basal layer was much less obvious than that with 64K antiserum. In contrast, 43K keratin was distributed throughout the whole epidermis including the basal layer, and no diminished staining with dilution of the antiserum was observed. These results suggest that an immunofluorescence technique using anti‐large M.W. keratin antisera, especially 64K antiserum with a certain degree of dilution, could be used to study the differentiated cells of the epidermis.DEAE‐cellulose column chromatography of keratins revealed that 64K keratin, along with some of the smaller M.W. keratins, was eluted at a low concentration of KCl and could be separated from most of the smaller M.W. keratins, which were eluted at higher concentrations of KCl. In the first process of stratum corneum fibrous protein extraction, 64K protein was observed to be extracted with neutral Tris‐HCl buffer. This protein was found to be immuno‐reactive with the antiserum to 64K keratin which had been extracted with Tris‐HCl buffer containing urea and mercaptoethanol. It is suggested that the biochemical properties of large M.W. keratin are somewhat different from those of smaller M.W. keratins.
EFFECTS OF CALCIUM AND CALCIUM‐IONOPHORE ON THE OUTGROWING EPIDERMIS —POSSIBLE ACTIVATION OF EPIDERMAL PHOSPHOLIPASE A2—
Authors: Aoyagi Takashi , Umeda Katsuko , Kato Naoko , Adachi Riri , Fukaya Toru , Nemoto Osamu , Kobayashi Hitoshi , Miura Yusho 
Published: April 2015

ABSTRACTUsing an in vitro explant culture system of pig skin, the effects of calcium and calcium‐ionophore on the outgrowing epidermis were studied. The optimum concentration of calcium on the rates of epidermal outgrowth was 1.0 mM and that of mitosis was around 1.2 mM. Ionophore A23187 (Ionophore) significantly stimulated both the rates of epidermal outgrowth and the mitosis of the outgrowing epidermis. This stimulation was partially blocked by the addition of hydrocortisone.The cyclo‐oxygenation products, prostaglandins, failed to stimulate the rate of epidermal outgrowth. It appeared that the stimulatory effect of Ionophore on the outgrowing epidermis was due either to the activation of phospholipase A2 or to the activation of the lipoxygenase pathway.
STUDIES OF CELL AND ORGAN CULTURE OF PSORIATIC AND NORMAL EPIDERMIS IN VITRO
Authors: Nakayama Juichiro , Yasumoto Shigeru , Takagi Yasuyuki , Nagae Shonosuke , Yashima Yutaka , Urabe Harukuni 
Published: April 2015

ABSTRACTInvolved and noninvolved epidermis from 15 psoriatic patients, and epidermis from 12 normal individuals were grown under various conditions in vitro as cell and organ cultures. In the organ cultures of normal or noninvolved psoriatic skin, epidermal cells migrated around the dermis and differentiated. In psoriatic organ cultures, progressive necrotic changes were observed in the spinous cells including gradual detachment of the spinous layer from the dermis. In spite of these necrotic changes, newly‐grown basal cells which appeared on the dermal tissue in the 9 to 12‐day psoriatic explants clearly incorporated 3H‐thymidine into nuclear DNA. By electron microscopy these cells were observed to have numerous ribosomes, a few mitochondria, tonofilament‐like structures in the cytoplasm, and no hemi‐desmosomes. Dissociated epidermal cells from the involved psoriatic skin failed to grow on plastic dishes. When these cells were plated on dishes coated with increasing amounts of collagen, the rate of cellular attachment was proportional to the thickness of the collagen coating, but they were unable to grow and form epidermal monolayers. In contrast, normal and noninvolved psoriatic epidermal cells attached to the dishes regardless of the presence of collagen and formed differentiated epidermal monolayers.
LOW‐TITER AGGLUTININS TO SPOROTHRIX SCHENCKII IN NONSPOROTRICHOTIC HUMAN AND ANIMAL SERA
Authors: Ishizaki Hiroshi , Nakamura Yoichi , Hasegawa Atsuhiko 
Published: April 2015

ABSTRACTLow‐titer agglutinins to Sporothrix schenckii were demonstrated in the sera of dogs, cats, rats (Wistar), guinea pigs, hamsters, white rabbits, swine, horses, cattle, sheep, chickens, and goats as well as in the human sera of nonsporotrichotic individuals. It is hypothesized that the presence of agglutinins is due to a response to various bacteria which share common antigens with S. schenckii.
EFFECT OF SERUM ALBUMIN ON GROWTH OF STAPHYLOCOCCUS AUREUS IN SYNTHETIC TISSUE CULTURE MEDIUM
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

ABSTRACTThe effect of bovine serum albumin on the growth of Staphylococcus aureus Smith in a synthetic tissue culture medium was investigated. The growth was almost the same when the albumin was added at the concentrations below 0.01 % or over 1.0%. At concentrations of albumin from 0.01 to 1.0%, a linear correlation was seen between each doubling time and the maximum growth level on the linear scale, and the albumin concentration on the logarithmic scale. It may be that Staphylococcus aureus present on the skin easily colonize and then form suppurative lesions after accumulation of serum‐like fluid by trauma or destruction of the skin or its appendages.
THE GLYCOLIPIDS OF NORMAL HUMAN SKIN
Authors: Hamanaka Sumiko , Asagami Chidori , Nishioka Kazue , Fujita Hidesuke 
Published: April 2015

ABSTRACTGlucosylceramide (CMH), lactosylceramide (CDH), ***trihexosylceramide (CTH), Globoside I, GM3, and GD3 were detected as constituent sphingoglycolipids of normal human skin, using Unisil, DEAE‐Sephadex, Iatrobeads column chromatography, and reversed‐phase chromatography. These procedures enabled isolation of glycolipids from even small amounts of skin.
THE MESHWORK STRUCTURE OF THE DERMOEPIDERMAL JUNCTION AS REVEALED BY TANGENTIALLY CUT SECTIONS IN A CASE OF PILAR TUMOR OF THE SCALP–HAS THE JUNCTION MANY PORES?
Authors: Ono Tomomichi , Sakazaki Yoshikado , Mutoh Kouichiro , Mera Shuji 
Published: April 2015

ABSTRACTThe dermo‐epidermal junction (parenchymal‐stromal junction) of a case of pilar tumor of the scalp was examined ultrastructurally.Although the basal lamina showed the same picture as the normal basal lamina, there were areas which were cut tangentially.The tangentially cut sections revealed a meshwork structure; this mesh work was related to the half desmosomes while the pores appeared to represent areas not related to the half desmosomes. The pore portions showed tumor cell components, lamina lucida, and dermal components.
ERYTHEMA INFECTIOSUM
Authors: Hata Seiichiro , Baba Koichi , Yabuuchi Hyakuji 
Published: April 2015

ABSTRACTAn outbreak of erythema infectiosum occurred in an orphanage housing a total of 99 infants and children; 37 were affected. Attack rates were 5% in infants less than one year old, 17% in one year olds, 50% in 2 year olds, and 37% throughout the range between 0 and 5 years.According to previous reports, large outbreaks of the disease occur in elementary schools but children of preschool age are less susceptible. We observed a high attack rate in younger children. The closed situation probably permitted the intensive contact among children needed to develop this high incidence.
AN UNUSUAL FORM OF SUTTON'S PHENOMENON
Authors: Matsubara Motoo , Maruo Michiru , Nonomura Kyoko , Inoue Yuji , Kobayashi Kazuo , Yasuno Hirokazu , Ueda Keiichi 
Published: April 2015

ABSTRACTAn unusual form of “Sutton's hypomelanotic phenomenon” was found in a 37‐year‐old man who developed hypomelanosis in a naevus pigmentosus pilliferus during the course of generalized vitiligo. Depigmentation was confined, unlike the ordinary halo phenomenon, to the interfollicular epidermis, thus leaving the hypermelanosis on the follicular area (acrotrichium) where augmented basal pigmentation was confirmed by histological examination. Lymphocytic infiltration toward naevus cells was also observed. He had a positive thyroid test (x100) and microsome test (x6400), but thyroid function was within normal limits. This phenomenon is considered to be similar to Sutton's phenomenon.
ABNORMAL DNA HISTOGRAMS OF STROMA CELLS IN PATIENTS WITH TUBEROUS SCLEROSIS
Authors: Ishibashi Yasumasa , Inoue Yukiko , Takehara Kazuhiko , Kukita Atsushi , Murakami Yasufumi , Hanaoka Fumio , Yamada Masa‐Atsu 
Published: April 2015

ABSTRACTExplant cultures were attempted from the so‐called adenoma sebaceum in the face and a plaque lesion on the neck observed in three genetically related cases (three males twelve, fifteen, and 42 years old) with Pringle's disease, tuberous sclerosis. Then the DNA content of outgrown non‐epithelial cells (NEC), or stroma cells, derived from the respective tissues (10,000 nuclei and 15,000 cells) were investigated by a flow cytofluorometer.1) DNA histograms per cell showed no remarkable differences between patients or lesions. On the other hand, 2) DNA histograms per nucleus did show certain differences. Among similar lesions, higher degrees of involvement showed more remarkable differences. 3) The NEC from the mulberry‐shaped tumor (adenoma sebaceum) showed obviously reduced peaks of 2C and 4C, and an increased width and increase in cells with their DNA content corresponding to the S‐phase. In addition, 4) there were no findings suggesting the presence of cells with excessive DNA content over 4C.
DETERMINATION OF SUITABLE CONCENTRATIONS FOR PATCH TESTING OF VARIOUS FRAGRANCE MATERIALS
Authors:   MID‐JAPAN CONTACT DERMATITIS RESEARCH GROUP
Published: April 2015

ABSTRACTTwelve fragrance materials were patch tested on patients with various facial dermatoses at three different concentrations to determine suitable concentrations of these materials for patch testing.The results indicate that 2% rose oil, 10% oil of bergamot, 5% jasmine absolute, 10% ylang ylang oil, 5% benzyl alcohol, 2% benzyl salicylate, 2% sandalwood oil, 2% α and β santalol, 2% isobornyl cyclohexanol, 5% hydroxycitronellal, 5% eugenol and 1% trans‐isoeugenol in white petrolatum are the optimal concentrations for testing, showing good accord with those recommended by ICDRG except for jasmine absolute, ylang ylang oil, and trans‐isoeugenol, which should be retested at concentrations lower than the range used in the present study.