bluuebunny/crossref_metadata_2025_split huggingface dataset:
PUF-8 Functions Redundantly with GLD-1 to Promote the Meiotic Progression of Spermatocytes inCaenorhabditis elegans
Authors: Priti Agarwal , Subramaniam Kuppuswamy 
Published: June 2015

AbstractSuccessful meiotic progression of germ cells is crucial for gametogenesis. Defects in this process affect proper genetic transmission and sometimes lead to tumor formation in the germline. In Caenorhabditis elegans, the RNA-binding protein GLD-1 is essential for the meiotic development of oocytes. However, its role during spermatogenesis has not been understood. Here, we show that GLD-1 functions redundantly with the PUF family protein PUF-8 to ensure proper meiotic development of spermatocytes. When grown at 20°—the standard laboratory temperature for C. elegans growth—primary spermatocytes in both gld-1 and puf-8 single-mutant males and hermaphrodites complete the meiotic divisions normally. By contrast, some of the gld-1; puf-8 double-mutant spermatocytes exit meiosis and form germ cell tumors in both sexes. During larval development, gld-1; puf-8 double-mutant germ cells begin to express the meiotic marker HIM-3, lose P granules, and form the sperm-specific membranous organelle, which are characteristics of developing spermatocytes. However, some of these cells quickly lose HIM-3 and form germ cell tumors that lack membranous organelle but contain P granules. Mutations that block meiotic progression at late pachytene or diakinetic stage fail to arrest the tumorigenesis, suggesting that the gld-1; puf-8 double-mutant spermatocytes exit meiosis prior to the completion of pachytene. Together, results presented here uncover a novel function for gld-1 in the meiotic development of spermatocytes in both hermaphrodites and males.
A Consensus Genetic Map forPinus taedaandPinus elliottiiand Extent of Linkage Disequilibrium in Two Genotype-Phenotype Discovery Populations ofPinus taeda
Authors: Westbrook Jared W , Chhatre Vikram E , Wu Le-Shin , Chamala Srikar , Neves Leandro Gomide , Muñoz Patricio , Martínez-García Pedro J , Neale David B , Kirst Matias , Mockaitis Keithanne , Nelson C Dana , Peter Gary F , Davis John M , Echt Craig S 
Published: June 2015

AbstractA consensus genetic map for Pinus taeda (loblolly pine) and Pinus elliottii (slash pine) was constructed by merging three previously published P. taeda maps with a map from a pseudo-backcross between P. elliottii and P. taeda. The consensus map positioned 3856 markers via genotyping of 1251 individuals from four pedigrees. It is the densest linkage map for a conifer to date. Average marker spacing was 0.6 cM and total map length was 2305 cM. Functional predictions of mapped genes were improved by aligning expressed sequence tags used for marker discovery to full-length P. taeda transcripts. Alignments to the P. taeda genome mapped 3305 scaffold sequences onto 12 linkage groups. The consensus genetic map was used to compare the genome-wide linkage disequilibrium in a population of distantly related P. taeda individuals (ADEPT2) used for association genetic studies and a multiple-family pedigree used for genomic selection (CCLONES). The prevalence and extent of LD was greater in CCLONES as compared to ADEPT2; however, extended LD with LGs or between LGs was rare in both populations. The average squared correlations, r2, between SNP alleles less than 1 cM apart were less than 0.05 in both populations and r2 did not decay substantially with genetic distance. The consensus map and analysis of linkage disequilibrium establish a foundation for comparative association mapping and genomic selection in P. taeda and P. elliottii.
Prevalence of Cardiovascular Autonomic Neuropathy in a Cohort of Patients With Newly Diagnosed Type 2 Diabetes: The Verona Newly Diagnosed Type 2 Diabetes Study (VNDS)
Authors: Zoppini Giacomo , Cacciatori Vittorio , Raimondo Daniele , Gemma Marialuisa , Trombetta Maddalena , Dauriz Marco , Brangani Corinna , Pichiri Isabella , Negri Carlo , Stoico Vincenzo , Bergamini Corinna , Targher Giovanni , Santi Lorenza , Thomaseth Karl , Bellavere Federico , Bonadonna Riccardo C. , Bonora Enzo 
Published: June 2015

OBJECTIVE
Cardiovascular autonomic diabetic neuropathy (CAN) is a serious complication of diabetes. No reliable data on the prevalence of CAN among patients with newly diagnosed type 2 diabetes are available. Therefore, the aim of this study was to estimate the prevalence of CAN among patients with newly diagnosed type 2 diabetes.

RESEARCH DESIGN AND METHODS
A cohort of 557 patients with newly diagnosed type 2 diabetes with cardiovascular autonomic test results available was selected. Early and confirmed neuropathy were assessed using a standardized methodology and their prevalences determined. A multivariate logistic regression analysis was modeled to study the factors associated with CAN.

RESULTS
In the entire cohort, the prevalence of confirmed CAN was 1.8%, whereas that of early CAN was 15.3%. Prevalence did not differ between men and women. In the multivariate analyses BMI results were independently and significantly associated with CAN after adjusting for age, sex, hemoglobin A1c, pulse pressure, triglyceride-to-HDL cholesterol ratio, kidney function parameters, and antihypertensive treatment.

CONCLUSIONS
CAN could be detected very early in type 2 diabetes. This study may suggest the importance of performing standardized cardiovascular autonomic tests after diagnosis of type 2 diabetes.
Five-Year Metabolic, Functional, and Safety Results of Patients With Type 1 Diabetes Transplanted With Allogenic Islets Within the Swiss-French GRAGIL Network
Authors: Lablanche Sandrine , Borot Sophie , Wojtusciszyn Anne , Bayle Francois , Tétaz Rachel , Badet Lionel , Thivolet Charles , Morelon Emmanuel , Frimat Luc , Penfornis Alfred , Kessler Laurence , Brault Coralie , Colin Cyrille , Tauveron Igor , Bosco Domenico , Berney Thierry , Benhamou Pierre-Yves ,   on behalf of the GRAGIL Network
Published: June 2015

OBJECTIVE
To describe the 5-year outcomes of islet transplantation within the Swiss-French GRAGIL Network.

RESEARCH DESIGN AND METHODS
Retrospective analysis of all subjects enrolled in the GRAGIL-1c and GRAGIL-2 islet transplantation trials. Parameters related to metabolic control, graft function, and safety outcomes were studied.

RESULTS
Forty-four patients received islet transplantation (islet transplantation alone [ITA] 24 patients [54.5%], islet after kidney [IAK] transplantation 20 patients [45.5%]) between September 2003 and April 2010. Recipients received a total islet mass of 9,715.75 ± 3,444.40 IEQ/kg. Thirty-four patients completed a 5-year follow-up, and 10 patients completed a 4-year follow-up. At 1, 4, and 5 years after islet transplantation, respectively, 83%, 67%, and 58% of the ITA recipients and 80%, 70%, and 60% of the IAK transplant recipients reached HbA1c under 7% (53 mmol/mol) and were free of severe hypoglycemia, while none of the ITA recipients and only 10% of the IAK transplant recipients met this composite criterion at the preinfusion stage. Thirty-three of 44 patients (75%) experienced insulin independence during the entire follow-up period, with a median duration of insulin independence of 19.25 months (interquartile range 2–58). Twenty-nine of 44 recipients (66%) exhibited at least one adverse event; 18 of 55 adverse events (33%) were possibly related to immunosuppression; and complications related to the islet infusion (n = 84) occurred in 10 recipients (11.9%).

CONCLUSIONS
In a large cohort with a 5-year follow-up and in a multicenter network setting, islet transplantation was safe and efficient in restoring good and lasting glycemic control and preventing severe hypoglycemia in patients with type 1 diabetes.
Risk factors for failure of single-level percutaneous endoscopic lumbar discectomy
Authors: Wang Hongwei , Zhou Yue , Li Changqing , Liu Jun , Xiang Liangbi 
Published: June 2015

OBJECT
Few studies have addressed surgical failures and complications following percutaneous endoscopic lumbar discectomy (PELD), and no previous study has investigated the risk factors that lead to surgical failure. The authors report their experience using PELD for single-level lumbar disc herniation (LDH) to provide insights into the rates of surgical failure and identify potential risk factors that lead to this complication.

METHODS
The authors retrospectively reviewed the medical records of 350 patients who underwent PELD for single-level LDH and identified 36 patients (10.3%) who underwent reoperation due to the failure of PELD.

RESULTS
Patients’ mean visual analog scale of pain scores and Oswestry Disability Index scores improved significantly from 6.6 ± 2.1 and 51.6 ± 19.4 preoperatively to 1.9 ± 1.4 and 28.3 ± 12.0, respectively, at 1 month postoperatively and 1.2 ± 1.1 and 9.3 ± 7.8, respectively, at 1 year postoperatively. The frequencies with which patients took analgesic medications significantly decreased from 74.6% preoperatively to 19.7% at 1 month postoperatively and 10.0% at 1 year postoperatively. Relatively older patients (p = 0.005) and those ≥ 60 years old (p = 0.001) experienced larger numbers of failures compared to younger patients. An analysis of potentially contributing comorbid conditions indicated that significantly more patients with diabetes were present in the PELD failure group (p = 0.017). As surgeons gained familiarity with the procedure, outcomes improved. The failure rate during the authors’ early use of the PELD technique (Cases 1–70) was 17.1%; the failure rate then fell to 5.7% (p = 0.034) (Cases 141–210) before finally stabilizing at 10.0% (Cases 211–280 and Cases 281–350).

CONCLUSIONS
The surgical failure rate following PELD for LDH was 10.3%. Older patients, elderly patients (age ≥ 60 years), and patients with diabetes were at increased risk of surgical failure of PELD, particularly in the early years of the procedure’s use.
The lateral infratrigeminal transpontine window to deep pontine lesions
Authors: Ferroli Paolo , Schiariti Marco , Cordella Roberto , Boffano Carlo , Nava Simone , La Corte Emanuele , Cavallo Claudio , Bauer Dario , Castiglione Melina , Broggi Morgan , Acerbi Francesco , Broggi Giovanni 
Published: June 2015

OBJECT
Surgery of brainstem lesions is increasingly performed despite the fact that surgical indications and techniques continue to be debated. The deep pons, in particular, continues to be a critical area in which the specific risks related to different surgical strategies continue to be examined. With the intention of bringing new knowledge into this important arena, the authors systematically examined the results of brainstem surgeries that have been performed through the lateral infratrigeminal transpontine window.

METHODS
Between 1990 and 2013, 29 consecutive patients underwent surgery through this window for either biopsy sampling or for removal of a deep pontine lesion. All of this work was performed at the Department of Neurosurgery of the Istituto Nazionale Neurologico "Carlo Besta", in Milan, Italy. A retrospective analysis of the findings was conducted with the intention of bringing further clarity to this important surgical strategy.

RESULTS
The lateral infratrigeminal transpontine window was exposed through 4 different approaches: 1) classic retrosigmoid (15 cases), 2) minimally invasive keyhole retrosigmoid (10 cases), 3) translabyrinthine (1 case), and 4) combined petrosal (3 cases). No deaths occurred during the entire clinical study. The surgical complications that were observed included hydrocephalus (2 cases) and CSF leakage (1 case). In 6 (20.7%) of 29 patients the authors encountered new neurological deficits during the immediate postoperative period. All 6 of these patients had undergone lesion removal. In only 2 of these 6 patients were permanent sequelae observed at 3 months follow-up. These findings show that 93% of the patients studied did not report any permanent worsening of their neurological condition after this surgical intervention.

CONCLUSIONS
This retrospective study supports the idea that the lateral infratrigeminal transpontine window is both a low-risk and safe corridor for either biopsy sampling or for removal of deep pontine lesions.
The effectiveness of percutaneous balloon compression in the treatment of trigeminal neuralgia in patients with multiple sclerosis
Authors: Martin Sean , Teo Mario , Suttner Nigel 
Published: June 2015

OBJECT
Trigeminal neuralgia (TN) is more common in multiple sclerosis (MS) patients than in the general population and among the former has an incidence of approximately 2%. The pathophysiology of TN in MS patients is believed to be caused by a demyelinating plaque at the root entry zone, and therefore procedures that cause direct nerve damage are thought to be the most effective surgical modality. The authors aimed to compare the efficacy of percutaneous balloon compression (PBC) in TN patients with and without MS.

METHODS
Retrospectively collected clinical data on 80 consecutive patients who underwent 144 procedures and who received PBC forTN treatment between January 2000 and January 2010 were analyzed. The cohort included 17 MS and 63 non-MS patients.

RESULTS
The mean age at first operation was significantly younger in the MS group compared with the non-MS group (59 years vs 72 years, respectively, p < 0.0001). After a mean follow-up of 43 months (MS group) and 25 months (non-MS group), the symptom recurrence rate following the first operation was higher in the MS group compared with that in the non-MS group (86% vs 47%, respectively, p < 0.01). During long-term follow-up, more than 70% of MS patients required multiple procedures compared with only 44% of non-MS patients. Excellent or satisfactory outcomes were not significantly different between the MS and non-MS cohorts, respectively, at 1 day postoperatively (82% vs 91%, p = 0.35), 3 months postoperatively (65% vs 81%, p = 0.16), and at last follow-up (65% vs 76%, p = 0.34). A similar incidence of postoperative complications was observed in the 2 groups.

CONCLUSIONS
PBC is effective in the treatment of trigeminal neuralgia in patients with MS, but, compared with that in non-MS patients, symptom recurrence is higher and requires multiple procedures.
Improved capture of adverse events after spinal surgery procedures with a longitudinal administrative database
Authors: Veeravagu Anand , Cole Tyler S. , Azad Tej D. , Ratliff John K. 
Published: June 2015

OBJECT
The significant medical and economic tolls of spinal disorders, increasing volume of spine surgeries, and focus on quality metrics have made it imperative to understand postoperative complications. This study demonstrates the utility of a longitudinal administrative database for capturing overall and procedure-specific complication rates after various spine surgery procedures.

METHODS
The Thomson Reuters MarketScan Commercial Claims and Encounters and the Medicare Supplemental and Coordination of Benefits database was used to conduct a retrospective analysis of longitudinal administrative data from a sample of approximately 189,000 patients. Overall and procedure-specific complication rates at 5 time points ranging from immediately postoperatively (index) to 30 days postoperatively were computed.

RESULTS
The results indicated that the frequency of individual complication types increased at different rates. The overall complication rate including all spine surgeries was 13.6% at the index time point and increased to 22.8% at 30 days postoperatively. The frequencies of wound dehiscence, infection, and other wound complications exhibited large increases between 10 and 20 days postoperatively, while complication rates for new chronic pain, delirium, and dysrhythmia increased more gradually over the 30-day period studied. When specific surgical procedures were considered, 30-day complication rates ranged from 8.6% in single-level anterior cervical fusions to 27.3% in multilevel combined anterior and posterior lumbar spine fusions.

CONCLUSIONS
This study demonstrates the usefulness of a longitudinal administrative database in assessing postoperative complication rates after spine surgery. Use of this database gave results that were comparable to those in prospective studies and superior to those obtained with nonlongitudinal administrative databases. Longitudinal administrative data may improve the understanding of overall and procedure-specific complication rates after spine surgery.
The safety and efficacy of use of low-molecular-weight heparin in pediatric neurosurgical patients
Authors: Gonda David D. , Fridley Jared , Ryan Sheila L. , Briceño Valentina , Lam Sandi K. , MBA MD , Luerssen Thomas G. , Jea Andrew 
Published: June 2015

OBJECT
Low-molecular-weight heparins (LMWHs), mainly enoxaparin, offer several advantages over standard anticoagulation therapies such as unfractionated heparin and warfarin, including predictable pharmacokinetics, minimal monitoring, and subcutaneous administration. The purpose of this study was to determine the safety and efficacy of LMWHs in pediatric neurosurgical patients.

METHODS
A retrospective study was performed with patients 18 years old or younger who were admitted to the Pediatric Neurosurgery Service at Texas Children's Hospital and treated with LMWH for either therapeutic or prophylactic purposes between March 1, 2011, and December 30, 2013. Demographic and clinical features and outcomes were recorded.

RESULTS
LMWH was administered for treatment of venous thromboembolic events (VTEs) in 17 children and for prophylaxis in 24 children. Clinical resolution of VTEs occurred in 100% (17 of 17) of patients receiving therapeutic doses of LMWH. No patient receiving prophylactic doses of LMWH developed a new VTE. Major or minor bleeding complications occurred in 18% (3 of 17 children) and 4% (1 of 24 children) of those receiving therapeutic and prophylactic doses, respectively. All 4 patients who experienced hemorrhagic complications had other bleeding risk factors—i.e., coagulopathies and antiplatelet medications.

CONCLUSIONS
LMWH seems to be safe and efficacious for both management and prophylaxis of VTEs in pediatric neurosurgery. However, pediatric practitioners should be aware of higher risk for bleeding complications with increasing doses of LMWH, especially in patients with preexisting bleeding disorders or concurrent use of antiplatelet agents.
Axial interbody arthrodesis of the L5–S1 segment: a systematic review of the literature
Authors: Schroeder Gregory D. , Kepler Christopher K. , MBA MD , Vaccaro Alexander R. 
Published: June 2015

OBJECT
The object of this study was to determine the fusion rate and safety profile of an axial interbody arthrodesis of the L5–S1 motion segment.

METHODS
A systematic search of MEDLINE was conducted for literature published between January 1, 2000, and August 17, 2014. All peer-reviewed articles related to the fusion rate of L5–S1 and the safety profile of an axial interbody arthrodesis were evaluated.

RESULTS
Seventy-four articles were identified, but only 15 (13 case series and 2 retrospective cohort studies) met the study inclusion criteria. The overall pseudarthrosis rate at L5–S1 was 6.9%, and the rate of all other complications was 12.9%. A total of 14.4% of patients required additional surgery, and the infection rate was 5.4%. Deformity studies reported a significantly increased rate of complications (46.3%), and prospectively collected data demonstrated significantly higher complication (36.8%) and revision (22.6%) rates. Lastly, studies with a conflict of interest reported lower complication rates (12.4%).

CONCLUSIONS
A systematic review of the literature indicates that an axial interbody fusion performed at the lumbosacral junction is associated with a high fusion rate (93.15%) and an acceptable complication rate (12.90%). However, these results are based mainly on retrospective case series by authors with a conflict of interest. The limited prospective data available indicate that the actual fusion rate may be lower and the complication rate may be higher than currently reported.
Mutations économiques et sociales en Flandre au cours de la phase proto-industrielle, 1650-1850
Authors: Vandenbroeke Christian 
Published: June 2015

Jusqu'au troisième quart du XVI ème siècle, la population flamande connaissait une prospérité relativement élevée. La culture intensive et l'extension de l'industrie linière étaient à la base de ce développement favorable. L'industrie linière représentait alors 20 à 25% par rapport au produit physique. Il est dès lors absurde d'assimiler exclusivement l'industrie rurale à un procès de paupérisation.
L'atteinte au pouvoir d'achat ne se manifesta que vers la fin du XVIIIème siècle et le début du XIXème siècle. Par des salaires réduits et une durée de travail prolongée on s'efforçait alors à résister à la concurrence étrangère et aux innovations techniques. Dès que la croissance économique diminua à la fin de l'Ancien Régime, le comportement démographique se modifia et la croissance de la population ralentit à cause d'une nuptialité plus restrictive.
La filiation comme incarnation
Authors: Berlanda Thierry 
Published: June 2015

Je traite ici la question de la filiation, notamment du point de vue original de la phénoménologie de Henry. Cette question a été traitée par Henry lui-même et m’est apparue comme l’une des plus fondamentales, si ce n’est la plus fondamentale de sa pensée, et donc peut-être de la pensée en général (étant donné la place éminente qu’y occupe Henry). Il s’est donc imposé à moi de tenter de la reprendre, bien sûr trop rapidement, depuis ses prémisses historiques, afin de montrer en quoi elle trouve dans la phénoménologie henryenne une sorte d’achèvement. Cette synthèse a donc pour vocation de dégager les moments ontologico-phénoménologiques fondant la possibilité même de ce qu’est un fils, et aussi, ce qui ne revient pas au même, de ce qu’est « être fils ».
À quoi rêvent les analystes ?
Authors: Neuquelman-Denis Fabienne 
Published: June 2015

Ce travail porte sur les rêves de l’analyste en cours de cure. Il étudie les conditions de leur validité et de leur pertinence pour le processus à l’œuvre, le travail analytique, ainsi que pour le patient. Il met en évidence l’arrière-plan théorique qui sous-tend la prise en compte de ce type de rêve. Enfin, il articule ces rêves à la problématique du « guérisseur blessé ». Une vignette clinique clôt le propos.
Du meurtre du père au maître à penser : paternité héritée, paternité élective
Authors: Wunenburger Jean-Jacques 
Published: June 2015

La filiation n’est pas seulement un processus biologique mais donne lieu à des métaphorisations et à des mimétismes symboliques de la paternité et de la maternité. Une figure parentale est d’abord un héritage subi, qu’on intériorise jusqu’à s’y dissoudre ou contre lequel l’enfant se révolte (meurtre du père, haine de la mère), mais elle peut aussi devenir une figure d’autorité et d’amour choisie, adoptée. On peut adopter un enfant mais qu’est-ce qu’adopter une parentalité ? Qu’est-ce que échanger une parentalité naturelle contre une affiliation de désir ? On se focalisera, à titre d’exemple, sur la figure des fils dans leur relation aux pères. Comment la relation héritée au père peut-elle être remplacée ou complétée par une(s) élection d’un ou plusieurs pères symboliques ? En quoi ce « transfert » produit-il une libération ou un assujettissement spirituel ? Qu’est-ce qu’un directeur de conscience, un père spirituel, un maître à penser, une idole ? Quels bénéfices et risques accompagnent cette paternité symbolique ? En quoi la paternité multiple peut-elle enrichir la filiation ? Faut-il avoir un père ou des pères ? Quelle est la fonction symbolique de l’image du père, source de mon identité mimétique ou matrice de médiation et de formation d’une identité nouvelle dans un processus d’individuation ? À quelles conditions l’image du père conduit-elle vers le Père archétypal ? Comment penser la relation multiple aux pères et à l’imago Dei au sens de Jung ?