bluuebunny/crossref_metadata_2025_split huggingface dataset:
High genetic diversity and geographic subdivision of three lance nematode species (Hoplolaimus spp.) in the United States
Authors: Holguin Claudia M. , Baeza Juan A. , Mueller John D. , Agudelo Paula 
Published: July 2015

AbstractLance nematodes (Hoplolaimus spp.) feed on the roots of a wide range of plants, some of which are agronomic crops. Morphometric values of amphimictic lance nematode species overlap considerably, and useful morphological characters for their discrimination require high magnification and significant diagnostic time. Given their morphological similarity, these Hoplolaimus species provide an interesting model to investigate hidden diversity in crop agroecosystems. In this scenario, H. galeatus may have been over‐reported and the related species that are morphologically similar could be more widespread in the United States that has been recognized thus far. The main objectives of this study were to delimit Hoplolaimus galeatus and morphologically similar species using morphology, phylogeny, and a barcoding approach, and to estimate the genetic diversity and population structure of the species found. Molecular analyses were performed using sequences of the cytochrome c oxidase subunit 1 (Cox1) and the internal transcribed spacer (ITS1) on 23 populations. Four morphospecies were identified: H. galeatus, H. magnistylus, H. concaudajuvencus, and H. stephanus, along with a currently undescribed species. Pronounced genetic structure correlated with geographic origin was found for all species, except for H. galeatus. Hoplolaimus galeatus also exhibited low genetic diversity and the shortest genetic distances among populations. In contrast, H. stephanus, the species with the fewest reports from agricultural soils, was the most common and diverse species found. Results of this project may lead to better delimitation of lance nematode species in the United States by contributing to the understanding the diversity within this group.
Maternal effects on offspring size and number in mosquitofish, Gambusia holbrooki
Authors: O'Dea Rose E. , Vega‐Trejo Regina , Head Megan L. , Jennions Michael D. 
Published: July 2015

AbstractGiven a trade‐off between offspring size and number, all mothers are predicted to produce the same optimal‐sized offspring in a given environment. In many species, however, larger and/or older mothers produce bigger offspring. There are several hypotheses to explain this but they lack strong empirical support. In organisms with indeterminate growth, there is the additional problem that maternal size and age are positively correlated, so what are their relative roles in determining offspring size? To investigate this, we measured the natural relationship between maternal and offspring size in a wild population of Gambusia holbrooki (eastern mosquitofish), and experimentally disentangled the effects of maternal age and size on offspring size in the laboratory. In combination, our data indicate that the relationship between maternal and offspring size is nonlinear. Small mothers seem to produce larger than average offspring due to integer effects associated with very small broods. For extremely large mothers, which were only sampled in our wild data, these larger than average offspring may result from greater maternal resources or age effects. However, maternal age had no effect on offspring size or number in the laboratory experiment. Our results highlight the importance of sampling the full size–range of mothers when investigating maternal effects on offspring size. They also point to the difficulty of experimentally manipulating maternal size, because any change in size is invariably associated with a change in at least one factor affecting growth (be it temperature, food availability, or density) that might also have an indirect effect on offspring size.
An automated shotgun lipidomics platform for high throughput, comprehensive, and quantitative analysis of blood plasma intact lipids
Authors: Surma Michal A. , Herzog Ronny , Vasilj Andrej , Klose Christian , Christinat Nicolas , Morin‐Rivron Delphine , Simons Kai , Masoodi Mojgan , Sampaio Julio L. 
Published: July 2015

Blood plasma has gained protagonism in lipidomics studies due to its availability, uncomplicated collection and preparation, and informative readout of physiological status. At the same time, it is also technically challenging to analyze due to its complex lipid composition affected by many factors, which can hamper the throughput and/or lipidomics coverage. To tackle these issues, we developed a comprehensive, high throughput, and quantitative mass spectrometry‐based shotgun lipidomics platform for blood plasma lipid analyses. The main hallmarks of this technology are (i) it is comprehensive, covering 22 quantifiable different lipid classes encompassing more than 200 lipid species; (ii) it is amenable to high‐throughput, with less than 5 min acquisition time allowing the complete analysis of 200 plasma samples per day; (iii) it achieves absolute quantification, by inclusion of internal standards for every lipid class measured; (iv) it is highly reproducible, achieving an average coefficient of variation of <10% (intra‐day), approx. 10% (inter‐day), and approx. 15% (inter‐site) for most lipid species; (v) it is easily transferable allowing the direct comparison of data acquired in different sites. Moreover, we thoroughly assessed the influence of blood stabilization with different anticoagulants and freeze‐thaw cycles to exclude artifacts generated by sample preparation.Practical applications: This shotgun lipidomics platform can be implemented in different laboratories without compromising reproducibility, allowing multi‐site studies and inter‐laboratory comparisons. This possibility combined with the high‐throughput, broad lipidomic coverage and absolute quantification are important aspects for clinical applications and biomarker research.This MS‐based automated shotgun lipidomics platform for comprehensive analysis of the blood plasma lipidome (covering 22 lipid classes) achieves high inter‐day and inter‐site reproducibility and accuracy and enables unprecedented large scale lipidomics studies.
Acyclic Pseudopeptidic Hosts as Molecular Receptors and Transporters for Anions
Authors: Martí Inés , Burguete M. Isabel , Gale Philip A. , Luis Santiago V. 
Published: July 2015

AbstractThe synthesis and characterization of different pseudopeptidic hosts derived from open chain systems with C2 and C3 symmetry has been achieved. These receptors are based on structures containing phenylalanine amino acid residues and amine, amide, urea and thiourea groups as the structural elements providing their capability to interact with anions. The transport of chloride and organic anions of biological relevance (L‐lactate, L‐maleate and L‐aspartate) through lipid bilayer membranes has been studied for these receptors. The chloride‐binding properties of the selected hosts have been studied in solution by NMR titrations. In addition, in order to rationalize the results, lipophilicity studies have been carried out. It has been observed that transport activity is affected by the nature of the aliphatic central spacer and the side chains, the most active receptors being 9b, derived from a tripodal precursor with the shortest aliphatic side chain and 3c, derived from a bis(amino amide) but having the longest spacer. These hosts are more selective for the anion chloride than for the studied organic anions.
Characterisation of erosional features associated with tsunami terrains on rocky coasts of the Maltese islands
Authors: Mottershead Derek N. , Bray Malcolm J. , Soar Philip J. , Farres Paul J. 
Published: July 2015

AbstractIn recent years there has been a growing body of literature on depositional signatures associated with historic extreme waves on rocky coasts. Here, in the context of the Maltese islands, we place an innovative focus on evidence of erosional forms. The field evidence is concentrated along the NE flank of the islands at a topographically varied range of sites and up to an altitude of 13 m asl. A range of forms is broadly classified in terms of their morphologies and the forces responsible for their formation. Sockets, eroded scarps, scoured terrains, clifftop erosion scars, swept terrains and spillways are interpreted as consequences of overwashing of the landscape by an extreme wave or waves. These forms are shown to be controlled by flow intensity, topography and lithology, and especially rock bedding and jointing. They comprise the source areas for associated depositional evidence allowing transport paths to be estimated, and may significantly enhance the reconstruction of extreme wave events. It is likely that similar (and additional) erosional forms are present elsewhere in the Mediterranean domain, where comparable lithological and topographic situations are exposed to extreme waves. Copyright © 2015 John Wiley & Sons, Ltd.
Sublethal health effects in laboratory rodents from environmentally relevant exposures to oil sands contaminants
Authors: Rodríguez-Estival Jaime , North Michelle A. , Smits Judit E.G. 
Published: July 2015

Abstract

Increasing activity of oil sands extraction and processing in northern Alberta is marked by ongoing controversy about the nature and extent of associated environmental impacts. Bitumen contains a mixture of toxic chemicals, including metals and residual polycyclic aromatic hydrocarbons (PAHs), whose release into the environment poses a distinct risk to the surrounding environment, plus wildlife and human health. In the present study, the authors evaluated several subclinical biomarkers of exposure and effect to mixtures of metals (Pb, Cd, and Hg) and/or PAHs (3 alkylated forms) at environmentally relevant concentrations (100-fold and 10-fold higher than the maximum dissolved concentrations found in snow, to simulate a worst-case scenario), using laboratory mice as a model for future studies of small mammals in the wild. Both metals and alkyl-PAHs exposure were associated with 1) increased relative liver, kidney, and spleen size; 2) alterations in the homeostasis of the antioxidant vitamins A and E in liver; and 3) compromised glutathione redox status in testes, with results also indicating synergistic interactions from co-exposure. The combination of morphometric and oxidative stress biomarkers provide reliable and sensitive measures of the response to contaminant exposure in a mammalian model, suggesting associated physiological costs. Based on the present experimental study, the authors propose that wild small mammals will prove to be valuable sentinel species reflecting sublethal health effects from oil sands–related contaminants. The present study's results also present a basis for the interpretation of future field data. Environ Toxicol Chem 2015;34:2884–2897. © 2015 SETAC
Development of a recombinant human ovarian (BG1) cell line containing estrogen receptor α and β for improved detection of estrogenic/antiestrogenic chemicals
Authors: Brennan Jennifer C. , Bassal Arzoo , He Guochun , Denison Michael S. 
Published: July 2015

Abstract

Estrogenic endocrine-disrupting chemicals are found in environmental and biological samples, commercial and consumer products, food, and numerous other sources. Given their ubiquitous nature and potential for adverse effects, a critical need exists for rapidly detecting these chemicals. The authors developed an estrogen-responsive recombinant human ovarian (BG1Luc4E2) cell line recently accepted by the US Environmental Protection Agency (USEPA) and Organisation for Economic Co-operation and Development (OECD) as a bioanalytical method to detect estrogen receptor (ER) agonists/antagonists. Unfortunately, these cells appear to contain only 1 of the 2 known ER isoforms, ERα but not ERβ, and the differential ligand selectivity of these ERs indicates that the currently accepted screening method only detects a subset of total estrogenic chemicals. To improve the estrogen screening bioassay, BG1Luc4E2 cells were stably transfected with an ERβ expression plasmid and positive clones identified using ERβ-selective ligands (genistein and Br-ERβ-041). A highly responsive clone (BG1LucERβc9) was identified that exhibited greater sensitivity and responsiveness to ERβ-selective ligands than BG1Luc4E2 cells, and quantitative reverse-transcription polymerase chain reaction confirmed the presence of ERβ expression in these cells. Screening of pesticides and industrial chemicals identified chemicals that preferentially stimulated ERβ-dependent reporter gene expression. Together, these results not only demonstrate the utility of this dual-ER recombinant cell line for detecting a broader range of estrogenic chemicals than the current BG1Luc4E2 cell line, but screening with both cell lines allows identification of ERα- and ERβ-selective chemicals. Environ Toxicol Chem 2016;35:91–100. © 2015 SETAC
Efficient multigrid preconditioners for atmospheric flow simulations at high aspect ratio
Authors: Dedner Andreas , Müller Eike , Scheichl Robert 
Published: July 2015

SummaryMany problems in fluid modelling require the efficient solution of highly anisotropic elliptic partial differential equations (PDEs) in ‘flat’ domains. For example, in numerical weather and climate prediction, an elliptic PDE for the pressure correction has to be solved at every time step in a thin spherical shell representing the global atmosphere. This elliptic solve can be one of the computationally most demanding components in semi‐implicit semi‐Lagrangian time stepping methods, which are very popular as they allow for larger model time steps and better overall performance. With increasing model resolution, algorithmically efficient and scalable algorithms are essential to run the code under tight operational time constraints. We discuss the theory and practical application of bespoke geometric multigrid preconditioners for equations of this type. The algorithms deal with the strong anisotropy in the vertical direction by using the tensor‐product approach originally analysed by Börm and Hiptmair [Numer. Algorithms, 26/3 (2001), pp. 219–234]. We extend the analysis to three dimensions under slightly weakened assumptions and numerically demonstrate its efficiency for the solution of the elliptic PDE for the global pressure correction in atmospheric forecast models. For this, we compare the performance of different multigrid preconditioners on a tensor‐product grid with a semi‐structured and quasi‐uniform horizontal mesh and a one‐dimensional vertical grid. The code is implemented in the Distributed and Unified Numerics Environment, which provides an easy‐to‐use and scalable environment for algorithms operating on tensor‐product grids. Parallel scalability of our solvers on up to 20 480 cores is demonstrated on the HECToR supercomputer. Copyright © 2015 John Wiley & Sons, Ltd.
GABAA receptor deficits predict recovery in patients with disorders of consciousness: A preliminary multimodal [11C]Flumazenil PET and fMRI study
Authors: Qin Pengmin , Wu Xuehai , Duncan Niall W. , Bao Weiqi , Tang Weijun , Zhang Zhengwei , Hu Jin , Jin Yi , Wu Xing , Gao Liang , Lu Lu , Guan Yihui , Lane Timothy , Huang Zirui , Bodien Yelena G. , Giacino Joseph T. , Mao Ying , Northoff Georg 
Published: July 2015

AbstractObjectivesDisorders of consciousness (DoC)—that is, unresponsive wakefulness syndrome/vegetative state and minimally conscious state—are debilitating conditions for which no reliable markers of consciousness recovery have yet been identified. Evidence points to the GABAergic system being altered in DoC, making it a potential target as such a marker.Experimental designIn our preliminary study, we used [11C]Flumazenil positron emission tomography to establish global GABAA receptor binding potential values and the local‐to‐global (LTG) ratio of these for specific regions. These values were then compared between DoC patients and healthy controls. In addition, they were correlated with behavioral improvements for the patients between the time of scanning and 3 months later. Functional magnetic resonance imaging resting‐state functional connectivity was also calculated and the same comparisons made.Principal observationslobal GABAA receptor binding was reduced in DoC, as was the LTG ratio in specifically the supragenual anterior cingulate. Both of these measures correlated with behavioral improvement after 3 months. In contrast to these measures of GABAA receptor binding, functional connectivity did not correlate with behavioral improvement.ConclusionsOur preliminary findings point toward GABAA receptor binding being a marker of consciousness recovery in DoC. Hum Brain Mapp 36:3867–3877, 2015. © 2015 Wiley Periodicals, Inc.
Effects of sex and proficiency in second language processing as revealed by a large‐scale fNIRS study of school‐aged children
Authors: Sugiura Lisa , Ojima Shiro , Matsuba‐Kurita Hiroko , Dan Ippeita , Tsuzuki Daisuke , Katura Takusige , Hagiwara Hiroko 
Published: July 2015

AbstractPrevious neuroimaging studies in adults have revealed that first and second languages (L1/L2) share similar neural substrates, and that proficiency is a major determinant of the neural organization of L2 in the lexical‐semantic and syntactic domains. However, little is known about neural substrates of children in the phonological domain, or about sex differences. Here, we conducted a large‐scale study (n = 484) of school‐aged children using functional near‐infrared spectroscopy and a word repetition task, which requires a great extent of phonological processing. We investigated cortical activation during word processing, emphasizing sex differences, to clarify similarities and differences between L1 and L2, and proficiency‐related differences during early L2 learning. L1 and L2 shared similar neural substrates with decreased activation in L2 compared to L1 in the posterior superior/middle temporal and angular/supramarginal gyri for both sexes. Significant sex differences were found in cortical activation within language areas during high‐frequency word but not during low‐frequency word processing. During high‐frequency word processing, widely distributed areas including the angular/supramarginal gyri were activated in boys, while more restricted areas, excluding the angular/supramarginal gyri were activated in girls. Significant sex differences were also found in L2 proficiency‐related activation: activation significantly increased with proficiency in boys, whereas no proficiency‐related differences were found in girls. Importantly, cortical sex differences emerged with proficiency. Based on previous research, the present results indicate that sex differences are acquired or enlarged during language development through different cognitive strategies between sexes, possibly reflecting their different memory functions. Hum Brain Mapp 36:3890–3911, 2015. © 2015 Wiley Periodicals, Inc.
Intracranial recordings reveal transient response dynamics during information maintenance in human cerebral cortex
Authors: Noy Niv , Bickel Stephan , Zion‐Golumbic Elana , Harel Michal , Golan Tal , Davidesco Ido , Schevon Catherine A. , McKhann Guy M. , Goodman Robert R. , Schroeder Charles E. , Mehta Ashesh D. , Malach Rafael 
Published: July 2015

AbstractDespite an extensive body of work, it is still not clear how short term maintenance of information is implemented in the human brain. Most prior research has focused on “working memory”—typically involving the storage of a number of items, requiring the use of a phonological loop and focused attention during the delay period between encoding and retrieval. These studies largely support a model of enhanced activity in the delay interval as the central mechanism underlying working memory. However, multi‐item working memory constitutes only a subset of storage phenomena that may occur during daily life. A common task in naturalistic situations is short term memory of a single item—for example, blindly reaching to a previously placed cup of coffee. Little is known about such single‐item, effortless, storage in the human brain. Here, we examined the dynamics of brain responses during a single‐item maintenance task, using intracranial recordings implanted for clinical purpose in patients (ECoG). Our results reveal that active electrodes were dominated by transient short latency visual and motor responses, reflected in broadband high frequency power increases in occipito‐temporal, frontal, and parietal cortex. Only a very small set of electrodes showed activity during the early part of the delay period. Interestingly, no cortical site displayed a significant activation lasting to the response time. These results suggest that single item encoding is characterized by transient high frequency ECoG responses, while the maintenance of information during the delay period may be mediated by mechanisms necessitating only low‐levels of neuronal activations. Hum Brain Mapp 36:3988–4003, 2015. © 2015 Wiley Periodicals, Inc.
Does Overweight and Obesity Impact on Self‐Rated Health? Evidence Using Instrumental Variables Ordered Probit Models
Authors: Cullinan John , Gillespie Paddy 
Published: July 2015

AbstractThis paper, for the first time, presents estimates of the causal impact of overweight and obesity on self‐rated health (SRH) using instrumental variables (IV) econometric methods. While a number of previous studies have sought to better understand the determinants of SRH, there is no consensus in relation to the impact of overweight and obesity. Using data from a large nationally representative sample of Irish parents and their children, we estimate a range of ordered probit models to isolate the causal effect of overweight and obesity on SRH. Our data includes independently and objectively recorded weight and height measures for parents and their children and we instrument for parental body mass index (BMI) status using the BMI of a biological child. After controlling for a range of individual, socioeconomic, health and lifestyle related variables, we find that being overweight has a negligible impact on SRH, while being obese has a practically and statistically significant negative impact on SRH, with these effects most pronounced for those who are most obese. We find only minor differences in these effects across gender. Copyright © 2015 John Wiley & Sons, Ltd.
Reconstructed centennial variability of Late Holocene storminess from Cors Fochno, Wales, UK
Authors: ORME L. C. , DAVIES S. J. , DULLER G. A. T. 
Published: July 2015

ABSTRACTFuture anthropogenic climate forcing is forecast to increase storm intensity and frequency over Northern Europe, due to a northward shift of the storm tracks, and a positive North Atlantic Oscillation. However understanding the significance of such a change is difficult because the natural variability of storminess beyond the range of instrumental data is poorly known. Here we present a decadal‐resolution record of storminess covering the Late Holocene, based on a 4‐m‐long core taken from the peat bog of Cors Fochno in mid‐Wales, UK. Storminess is indicated by variations in the minerogenic content as well as bromine deposited from sea spray. Twelve episodes of enhanced storm activity are identified during the last 4.5 cal ka BP. Although the age model gives some uncertainty in the timings, it appears that storminess increased at the onset and close of North Atlantic cold events associated with oceanic changes, with reduced storm activity at their peak. Cors Fochno is strongly influenced by westerly moving storms, so it is suggested that the patterns were due to variations in the intensity of westerly airflow and atmospheric circulation during times when the latitudinal temperature gradient was steepened.