bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of peripheral cannabinoid and opioid receptors in β‐caryophyllene‐induced antinociception
Authors: Katsuyama S. , Mizoguchi H. , Kuwahata H. , Komatsu T. , Nagaoka K. , Nakamura H. , Bagetta G. , Sakurada T. , Sakurada S. 
Published: November 2012

AbstractBackground  β‐caryophyllene (BCP) is a common constitute of the essential oils of numerous spice, food plants and major component in Cannabis. The present study investigated the contribution of peripheral cannabinoid (CB) and opioid systems in the antinociception produced by intraplantar (i.pl.) injection of BCP. The interaction between peripheral BCP and morphine was also examined.Methods  The antinociceptive effect of i.pl. BCP was assayed by the capsaicin tests in mice. Antagonists for CB and opioid receptors, and antisera against β‐endorphin were injected peripherally prior to i.pl. injection of BCP. Morphine in combination with BCP was injected subcutaneously or intrathecally.ResultsThe i.pl. injection of BCP dose‐dependently attenuated capsaicin‐induced nociceptive response. The antinociceptive effect produced by BCP was prevented by pretreatment with AM630, a selective CB2 receptor antagonist, but not by AM251, a selective CB1 receptor antagonist. Pretreatment with naloxone, an opioid receptor antagonist, and β‐funaltrexamine, a selective μ‐opioid receptor antagonist, reversed the antinociceptive effect of BCP. Pretreatment with naloxone methiodide, a peripherally acting antagonist for opioid receptors and antisera against β‐endorphin, resulted in a significant antagonizing effect on BCP‐induced antinociception. Morphine‐induced antinociception was increased by a low dose of BCP. The increased effect of morphine in combination with BCP was antagonized significantly by pretreatment with naloxone.ConclusionsThe present results demonstrate that antinociception produced by i.pl. BCP is mediated by activation of CB2 receptors, which stimulates the local release from keratinocytes of the endogenous opioid β‐endorphin. The combined injection of morphine and BCP may be an alternative in treating chemogenic pain.
Characterization of atherosclerotic plaque depositions by Raman and FTIR imaging
Authors: Lattermann Annika , Matthäus Christian , Bergner Norbert , Beleites Claudia , Romeike Bernd F. , Krafft Christoph , Brehm Bernhard R. , Popp Jürgen 
Published: November 2012

AbstractSpectroscopy‐based imaging techniques can provide useful biochemical information about tissue samples. Here, we employ Raman and Fourier transform infrared (IR) imaging to characterize composition and constitution of atherosclerotic plaques of rabbits, fed with a high cholesterol diet. The results were compared with conventional light microscopy after staining with hematoxylin eosin, and elastica van Gieson. The spectral unmixing algorithm vertex component analysis was applied for data analysis and image reconstruction. IR microscopy allowed for differentiation between lipids and proteins in plaques of full aortic cross sections. Raman microscopy further discriminated cholesterol esters, cholesterol and triglycerides. FTIR and Raman images were recorded at a resolution near 20 micrometer per pixel for a large field of view. High resolution Raman images at 1 micrometer per pixel revealed structural details at selected regions of interest. The intima‐media and the lipid‐protein ratio were determined in five specimens for quantitation. These results correlate well with histopathology. The described method is a promising tool for easy and fast molecular imaging of atherosclerosis. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Study of Hepatitis B Prevalence in Parallel With the Most Frequent HBVGenotype in South Iranian Blood Donors
Authors: Karimabad Mojgan Noroozi , Hassanshahi Gholamhossein , Gharebaghiyan Ahmad , Sharifi Zohre 
Published: November 2012

BackgroundHepatitis B virus (HBV) is one of the leading causes of acute and chronic liver diseases and in turn responsible for a million of worldwide annual deaths. HBVhas been classified into eight main groups, designated as A–H with different geographical distribution. Some genotypes are associated with different clinical outcomes and particular viral mutations. Current study was aimed to investigate the genotype and prevalence of HBV in blood donors of south of Iran.MethodsThis experimental study investigated the prevalence of HBVpositivity in 198,289 Iranian blood donors from south of Iran by both ELISA‐ and PCR‐based methods. Within 198,289 donors, 120 HBsAg+ cases were selected, HBV‐DNAwas extracted, and the p gene sequences were amplified by nested‐PCR. The HBVgenotypes were determined by direct sequencing of the polymerase gene of HBV. Phylogenetic trees also were constructed by the neighbor‐joining (NJ) method.ResultsFindings of this study indicated that 0.184%, 0.329%, and 0.215% of blood donors were HBsAg+ among Isfahan, Kerman, and Yazd provinces, respectively. Only 69 (57.5%) cases of 120 HBsAg+ donors were HBV‐DNA+. Sequencing analysis revealed that all of HBV‐infected donors had the D genotype of HBV.ConclusionsIn conclusion, the prevalence of genotype D was 100% in Iranian HBVblood donors. These findings may have an impact on the immunological and genetic diagnosis of HBV, selection of diagnostic kits, and viral quality control panels to evaluate diagnostic methods.
Hsa‐miR‐196a2 Functional SNP is Associated With Severe Toxicity After Platinum‐Based Chemotherapy of Advanced Nonsmall Cell Lung Cancer Patients in a Chinese Population
Authors: Zhan Xiaoying , Wu Wenting , Han Baohui , Gao Ge , Qiao Rong , Lv Juan , Zhang Shuyu , Zhang Wei , Fan Weiwei , Chen Hongyan , Zhang Tianbao , Gu Aiqin , Shen Jie , Wu Qihan , Lu Daru 
Published: November 2012

BackgroundRs11614913 is a polymorphism in hsa‐miR‐196a2 reported to alter mature microRNA expression and function. This single‐nucleotide polymorphism (SNP) was reported to be associated with susceptibility and prognosis of lung cancer.MethodsIn this article, association study was performed to reveal the relation between SNP and response rate or severe toxicity after platinum‐based regimen in advanced nonsmall cell lung cancer patients.ResultsBy screening this polymorphism in 442 Chinese patients with MALDI‐TOF Mass Spectrometer, significantly higher occurrence of grade 3 or 4 overall toxicity (P = 0.02) in response to treatment was found in patients with homozygous CC. After stratified analyses, association between rs11614912 and overall toxicity existed, especially in individuals treated with gemcitabine (P = 0.006) or cisplatin (P = 0.008), and in male patients (P = 0.02) or younger patients (P = 0.01).ConclusionOur study confirmed that rs11614913 in hsa‐miR‐196a2 was associated with severe toxicity in lung cancer patients, and might help to improve individualized therapy in the future.
Mean Platelet Volume in Neonatal Sepsis
Authors: Oncel Mehmet Yekta , Ozdemir Ramazan , Yurttutan Sadik , Canpolat Fuat Emre , Erdeve Omer , Oguz Serife Suna , Uras Nurdan , Dilmen Ugur 
Published: November 2012

BackgroundThe aim of this study was to investigate any changes in mean platelet volume (MPV) in patients with neonatal sepsis (NS).MethodsSubjects were stratified into two groups: proven sepsis (Group 1a) and clinical sepsis (Group 1b). The control group (Group 2) consisted of healthy newborns matched for gestational age and birth weight.ResultsA total of 100 patients with NS (35 with proven sepsis and 65 with clinical sepsis) and 50 healthy controls were enrolled. A comparison of markers of sepsis obtained at baseline revealed white blood cell count (WBC), C‐reactive protein (CRP), interleukin‐6 (IL‐6), and MPV levels to be significantly higher in newborns with sepsis compared to healthy controls (P = 0.01, <0.001, <0.001, and 0.001, respectively). Mean baseline serum levels of CRP and MPV were significantly higher in Group 1a compared to Group 1b (P = 0.003, P = 0.007, respectively), whereas the difference between group with regards to baseline serum levels of IL‐6 and platelet count was statistically insignificant (P = 0.14, P = 0.28, respectively).ConclusionThis is the first study to demonstrate a statistically significant difference with regard to baseline MPV values between patients with sepsis (proven or clinical) and healthy controls.
Treatment efficacy of DNA lipid nanocapsules and DNA multimodular systems after systemic administration in a human glioma model
Authors: David Stephanie , Montier Tristan , Carmoy Nathalie , Resnier Pauline , Clavreul Anne , Mével Mathieu , Pitard Bruno , Benoit Jean‐Pierre , Passirani Catherine 
Published: November 2012

AbstractBackgroundWe previously developed different types of DNA nanocarriers for systemic administration. Recently, the biodistribution profiles of these intravenously administered nanocarriers, DNA lipid nanocapsules (LNCs) and different multimodular systems (MMS), were analysed in healthy mice using in vivo biofluorescence imaging.MethodsIn the present study, the experiments were performed in an ectopic human U87MG glioma model in nude mice. First, the biodistribution profiles of intravenously administered multimodular systems delivering a plasmid DNA with a luciferase cassette were analysed using in vivo biofluorescence imaging. Afterwards, a systemic treatment with two long circulating DNA nanocarriers, poly(ethylene glycol) (PEG) DNA LNCs and galactose (GAL) DNA MMS dioleylamin‐succinyl paromomycin (DOSP) was performed on this glioma model using a plasmid encoding the herpes simplex virus thymidine kinase (HSV‐tk) and subsequent ganciclovir (GCV) treatment.ResultsThe biodistribution profiles of the different DNA nanocarriers on this glioma model were similar to those observed on healthy animals and varied in function of their cationic lipid composition and their surface characteristics. Furthermore, PEG DNA LNCs and GAL DNA MMS DOSP showed a specific accumulation and some luciferase expression in the tumour tissue. The systemic treatment using the HSV‐tk/GCV approach showed a tumour growth reduction compared to the nontreated mice cohort.ConclusionsThese results are in good accordance with those obtained previously with PEG DNA LNCs in a human melanoma mouse model and highlight the potential use of GAL DNA MMS DOSP and PEG DNA LNCs as future therapeutics in glioma and other cancers. Copyright © 2012 John Wiley & Sons, Ltd.
Chondromodulin‐I derived from the inner meniscus prevents endothelial cell proliferation
Authors: Fujii Masataka , Furumatsu Takayuki , Yokoyama Yusuke , Kanazawa Tomoko , Kajiki Yuya , Abe Nobuhiro , Ozaki Toshifumi 
Published: November 2012

AbstractThe meniscus is a fibrocartilaginous tissue that plays an important role in controlling complex biomechanics of the knee. A perimeniscal capillary plexus supplies the outer meniscus, whereas the inner meniscus is composed of avascular tissue. Anti‐angiogenic molecules, such as chondromodulin‐I (ChM‐I) and endostatin, have pivotal roles in preserving the avascularity of cartilage. However, the anti‐angiogenic role of ChM‐I is unclear in the meniscus. We hypothesized that the inner meniscus might maintain its avascular feature by expressing ChM‐I. Immunohistochemical analyses revealed that ChM‐I was mainly detected in the inner and superficial zones of the meniscus. On the other hand, endostatin distribution was similar between the inner and outer meniscus. In Western blot, ChM‐I was detected only in the inner meniscus, whereas endostatin was equally observed in both inner and outer menisci. In addition, ChM‐I concentration of the inner meniscus‐derived conditioned medium was higher than that of the outer meniscus‐derived medium. ChM‐I removal from the inner meniscus‐derived medium and functional blocking of ChM‐I significantly increased endothelial cell proliferation. In this study, we demonstrated that the inner meniscus contained larger amounts of ChM‐I, and that the inner meniscus‐derived ChM‐I inhibited endothelial cell proliferation. Our results suggest that ChM‐I may be a key anti‐angiogenic factor for maintaining the avascularity of the inner meniscus. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 538–543, 2013
The overexpression of SIRT1 inhibited osteoarthritic gene expression changes induced by interleukin‐1β in human chondrocytes
Authors: Matsushita Takehiko , Sasaki Hiroshi , Takayama Koji , Ishida Kazunari , Matsumoto Tomoyuki , Kubo Seiji , Matsuzaki Tokio , Nishida Kotaro , Kurosaka Masahiro , Kuroda Ryosuke 
Published: November 2012

AbstractIn this study, we examined the effects of overexpression of SIRT1 on IL‐1β‐induced gene expression changes in human chondrocytes to explore a protective role of SIRT1 in human chondrocytes. SIRT1 was overexpressed in human chondrocytes by expression plasmid under stimulation with IL‐1β. SIRT1 was also inhibited by siRNA under stimulation with IL‐1β. Gene expression changes were examined by real‐time PCR. The interaction of SIRT1 and p65 (NF‐κB) were examined by Western blotting. SIRT1, MMP‐13, and ADAMTS‐5 expressions in human cartilage were examined by immunohistochemistry. IL‐1β stimulation significantly up‐regulated MMP‐1, 2, 9, and 13 and ADAMTS‐5. Overexpression of SIRT1 significantly inhibited the up‐regulation of those genes caused by IL‐1β while the inhibition of SIRT1 further increased them. In addition, the overexpression of SIRT1 markedly reduced the IL‐1β‐induced acetylation of p65. SIRT1 expression was clearly detected in the non‐OA cartilage while MMP‐13 and ADAMTS‐5 were undetectable. In contrast, in the OA cartilage, SIRT1 expression was decreased while MMP‐13 and ADAMTS‐5 were increased. Our observations suggested that SIRT1 can play a protective role by suppressing IL‐1β‐induced expressions of cartilage‐degrading enzymes partially through the modulation of the NF‐κB pathway. SIRT1 overexpression might be a new therapeutic approach for OA. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 531–537, 2013
Kernel elongation in rice
Authors: Golam Faruq , Prodhan Zakaria H 
Published: November 2012

AbstractKernel elongation after cooking is an important character of fine rice and most rice consumers prefer length‐wise elongation. Although improvement of aromatic rice began early in the 1970s, until now the mechanisms and genetics of kernel elongation has remained unrevealed. Kernel elongation is considered as a physical phenomenon and is influenced by several physicochemical and genetic factors, including genotypes, aging temperature, aging time, water uptake, amylose content and gelatinization temperature. Recently the complete genetic map of fine rice has been created and the gene responsible for kernel length identified; moreover, this gene is tightly linked with the cooked kernel elongation trait. Several molecular markers linked with cooked kernel elongation have been developed. These tools will be helpful for the improvement of this important trait. For the proper study of cooked kernel elongation of rice, this review paper will provide the basis and directional materials for further studies.© 2012 Society of Chemical Industry
Improvement of lipopolysaccharide‐induced hepatic injuries and inflammation with mulberry extracts
Authors: Ou Ting‐Tsz , Kuo Chih‐Yi , Chyau Charng‐Cherng , Lee Huei‐Jane , Peng Jih‐Shin , Wang Chau‐Jong 
Published: November 2012

AbstractBACKGROUNDMulberry water extracts (MWEs), which contain polyphenols including anthocyanins, have been used in traditional Chinese edible food. The hepatoprotective effects and molecular mechanisms of MWEs on acute liver failure induced by lipopolysaccharides (LPS) were investigated in vivo.RESULTSRats were administered different doses of MWEs (0.5 and 1 g kg−1) 1 h before injection of LPS (5 mg kg−1) and then sacrificed 10 h after treatment with LPS. Liver function, inflammatory factors, oxidative stress index and hepatic histopathological alteration were examined in the rats with and without MWE treatment. Pretreatment with MWEs prevented LPS‐induced liver damage by preventing associated increases of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALKP), triglycerol (TG), cholesterol and low‐density lipoprotein/high‐density lipoprotein ratio. MWEs also suppressed oxidative stress to prevent the formation of hepatic malondialdehyde (MDA). Furthermore, the molecular mechanism involved in LPS‐induced liver injury was associated with reducing the expression of COX‐2, NF‐κB and iNOS in liver tissues.CONCLUSIONThe results support the investigation of MWEs as a therapeutic candidate for liver injuries and indicate that MWEs exhibit hepatoprotective activities via NF‐κB signaling.© 2012 Society of Chemical Industry
Very mild presentation in adult with classical cellular phenotype of ataxia telangiectasia
Authors: Worth Paul F. , Srinivasan Venkataramanan , Smith Anna , Last James I. , Wootton Laura L. , Biggs Paul M. , Davies Nicholas P. , Carney Ellen F. , Byrd Philip J. , Taylor A. Malcolm R. 
Published: November 2012

BackgroundThe major clinical feature of ataxia telangiectasia (A‐T) is severe progressive neurodegeneration with onset in infancy. This classical A‐T phenotype is caused by biallelic null mutations in the ATM gene, leading to the absence of ATM protein and increased cellular radiosensitivity. We report an unusual case of A‐T in a 41‐year‐old mother, A‐T210, who had very mild neurological symptoms despite complete loss of ATM protein.MethodsA neurological examination was performed, cellular radiosensitivity was assessed, and the ATM gene was sequenced. Skin fibroblasts and a lymphoblastoid cell line (LCL) were assayed for ATM protein expression and kinase activity.ResultsPatient A‐T210 showed mild chorea, dystonia, and gait ataxia, walked independently, and drove a car. LCL and skin fibroblasts were radiosensitive and did not express ATM protein. Two ATM‐null mutations were identified.ConclusionsThe severe neurodegeneration resulting from loss of ATM can be mitigated in some circumstances. © 2012 Movement Disorder Society
oct4‐EGFP reporter gene expression marks the stem cells in embryonic development and in adult gonads of transgenic medaka
Authors: Froschauer Alexander , Khatun Mst. Muslima , Sprott David , Franz Alexander , Rieger Christiane , Pfennig Frank , Gutzeit Herwig O. 
Published: November 2012

AbstractMaintenance of pluripotency in stem cells is tightly regulated among vertebrates. One of the key genes in this process is oct4, also referred to as pou5f1 in mammals and pou2 in teleosts. Pou5f1 evolved by duplication of pou2 early in the tetrapod lineage, but only monotremes and marsupials retained both genes. Either pou2 or pou5f1 was lost from the genomes of the other tetrapods that have been analyzed to date. Consequently, these two homologous genes are often designated oct4 in functional studies. In most vertebrates oct4 is expressed in pluripotent cells of the early embryo until the blastula stage, and later persist in germline stem cells until adulthood. The isolation and analysis of stem cells from embryo or adult individuals is hampered by the need for reliable markers that can identify and define the cell populations. Here, we report the faithful expression of EGFP under the control of endogenous pou2/oct4 promoters in transgenic medaka (Oryzias latipes). In vivo imaging in oct4‐EGFP transgenic medaka reveals the temporal and spatial expression of pou2 in embryos and adults alike. We describe the temporal and spatial patterns of endogenous pou2 and oct4‐EGFP expression in medaka with respect to germline and adult stem cells, and discuss applications of oct4‐EGFP transgenic medaka in reproductive and stem cell biology. Mol. Reprod. Dev. 80: 48–58, 2013. © 2012 Wiley Periodicals, Inc.
Thoracoamniotic shunting for fetal pleural effusions using a double‐basket shunt
Authors: Takahashi Yuichiro , Kawabata Ichiro , Sumie Masahiro , Nakata Masahiko , Ishii Keisuke , Murakoshi Takeshi , Katsuragi Shinji , Ikeda Tomoaki , Saito Mari , Kawamoto Hiroshi , Hayashi Satoshi , Sago Haruhiko 
Published: November 2012

ABSTRACTObjectiveTo describe the safety and efficacy of thoracoamniotic shunting for fetal pleural effusion using a double‐basket catheter with a very small diameter (1.47 mm).MethodIn this 2‐year multicenter, prospective single‐arm clinical study registered with the University hospital Medical Information Network (UMIN) Clinical Trials Registry (UMIN00001095); shunting was performed between 18w0d and 33w6d of gestation with this catheter in cases of fetal pleural effusions reaccumulating after thoracocentesis. The primary endpoint measures were maternal and fetal adverse effects and survival in the neonatal period.ResultsA total of 24 cases were included, of which 17 had hydrops (71%). The median gestational ages at shunting and delivery were 27.4 and 34.8 weeks, respectively. There were no fetal deaths, lung injuries, or severe maternal complications. Preterm rupture of the membranes occurred in 7/24 (29%) cases at a median of 62 days after the shunting. Preterm rupture of the membranes within 28 days of the procedure occurred in 1/24 (4%) cases. Catheter displacement towards the fetal thoracic cavity occurred in 4/42 (10%) cases. The overall survival rate was 79% (19/24), whereas it was 71% (12/17) in the cases with hydrops.ConclusionDrainage of fetal pleural effusions with a double‐basket shunt is safe and effective, and the shunt could be an alternative device. © 2012 John Wiley & Sons, Ltd.
Waist circumference, BMI, and lung function in 8‐year‐old children: The PIAMA birth cohort study
Authors: Bekkers Marga B.M. , Wijga Alet H. , de Jongste Johan C. , Kerkhof Marjan , Postma Dirkje , Gehring Ulrike , Smit Henriëtte A. , Brunekreef Bert 
Published: November 2012

AbstractBackgroundBody mass index (BMI) and waist circumference (WC) may be associated with lung function in children, as observed in adults.MethodsHeight, weight, waist circumference, and lung function (FVC and FEV1) were measured during a medical examination in 1,058 eight‐year‐old children participating in the Prevention and Incidence of Asthma and Mite Allergy (PIAMA) birth cohort study.ResultsAfter adjusting for height, age and other potential confounders large WC or high BMI (>90th percentile) were not associated with forced expiratory volume in 1 sec (FEV1) or forced vital capacity (FVC). In girls only, large WC was, independently of BMI, associated with 3.5% (95% confidence interval (CI): −6.4, −0.6) lower FEV1/FVC ratio in the model including WC and BMI. Girls with low BMI (<10th percentile) had 4.6% lower FEV1 (95% CI: −8.4, −0.6) and 5.1% lower FVC (95% CI: −8.8, −1.2) than girls with normal BMI. In boys, we did not observe associations between low or high BMI and lung function independent of WC, or between small or large WC and lung function after adjustment for BMI.ConclusionAt 8 years of age, a high BMI or large WC are not yet associated with FEV1 and FVC, indicating that this association may change over the course of life from childhood to adulthood. Pediatr Pulmonol. 2013; 48:674–682. © 2012 Wiley Periodicals, Inc.