bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving panicle exsertion of rice cytoplasmic male sterile line by combination of artificial microRNA and artificial target mimic
Authors: Chen Hao , Jiang Shan , Zheng Jie , Lin Yongjun 
Published: November 2012

SummaryThe adoption of hybrid rice caused the second leap in rice yield after the ‘green revolution’ and contributes substantially to food security of China and the world. However, almost all cytoplasmic male sterile lines (A lines) as females of hybrid rice have a natural deficiency of ‘panicle enclosure’, which blocks pollination between the A line and the fertility restorer line as the male (R line) of hybrid rice and decreases seed yield. In hybrid rice seed production, exogenous ‘920’ (the active ingredient is gibberellin A3) must be applied to eliminate or alleviate panicle enclosure of the A line; however, this not only increases production cost and pollutes the environment, it also decreases seed quality. In this study, we designed a transgenic approach to improve plant height and panicle exsertion of the A line to facilitate hybrid rice production and maintain the semi‐dwarf plant type of the hybrid. This approach comprising two components—artificial microRNA (amiRNA) and artificial target mimicry—can manipulate the differential expression of the endogenous Eui1 gene that is associated with rice internode elongation in the A line and the hybrid. amiRNA is a recently developed gene silencing method with high specificity, while target mimicry is a natural mechanism inhibiting the miRNA function that was also recently characterized. This approach provides a paradigm to tune the expression of endogenous genes to achieve the desired phenotype by combining amiRNA and artificial target mimicry technologies.
Newborn screening for cystic fibrosis in Serbia: A pilot study
Authors: Radivojevic Danijela , Sovtic Aleksandar , Minic Predrag , Grkovic Sanja , Guc‐Scekic Marija , Lalic Tanja , Miskovic Marijana 
Published: November 2012

AbstractBackgroundWe performed a pilot study of neonatal screening for cystic fibrosis (CF) in order to introduce it to the national screening program in Serbia.MethodsImmunoreactive trypsinogen (IRT) concentrations were analyzed in dried blood spot samples. Patients were recalled for repeated measurements in case of high IRT levels. Persisting high IRT levels resulted in DNA testing for the 29 most common mutations in the CF transmembrane regulator (CFTR) gene (IRT/IRT/DNA method). Sweat chloride measurements and clinical assessment were further performed for newly diagnosed patients.ResultsOf 1000 samples, three were initially positive and were further analyzed for the presence of the most common CFTR mutations in the Serbian population. DNA analysis revealed two patients being homozygous for F508del mutation. One sample was false positive, as the genetic test proved to be negative and associated with normal sweat chloride concentration and unremarkable clinical presentation.ConclusionsThe results of our pilot study justified the expanding of the routine neonatal screening program in Serbia with CF. Data could be used in future in order to obtain accurate incidence of CF and carrier prevalence in our country.
High‐resolution computational imaging of leaf hair patterning using polarized light microscopy
Authors: Pomeranz Marcelo , Campbell Jeffrey , Siegal‐Gaskins Dan , Engelmeier Jacob , Wilson Tyler , Fernandez Virginia , Brkljacic Jelena , Grotewold Erich 
Published: November 2012

SummaryThe leaf hairs (trichomes) on the aerial surface of many plant species play important roles in phytochemical production and herbivore protection, and have significant applications in the chemical and agricultural industries. Trichome formation in the model plant Arabidopsis thaliana also presents a tractable experimental system to study cell differentiation and pattern formation in plants and animals. Studies of this developmental process suggest that trichome positioning may be the result of a self‐forming pattern, emerging from a lateral inhibition mechanism determined by a network of regulatory factors. Critical to the continued success of these studies is the ability to quantitatively characterize trichome pattern phenotypes in response to mutations in the genes that regulate this process. Advanced protocols for the observation of changes in trichome patterns can be expensive and/or time consuming, and lack user‐friendly analysis tools. In order to address some of these challenges, we describe here a strategy based on polarized light microscopy for the quick and accurate measurement of trichome positions, and provide an online tool designed for the quantitative analyses of trichome number, density and patterning.
Complete Genome Sequence of a Recombinant Marek's Disease Virus Field Strain with One Reticuloendotheliosis Virus Long Terminal Repeat Insert
Authors: Su Shuai , Cui Ning , Cui Zhizhong , Zhao Peng , Li Yanpeng , Ding Jiabo , Dong Xuan 
Published: November 2012

ABSTRACTMarek's disease virus (MDV) Chinese strain GX0101, isolated in 2001 from a vaccinated flock of layer chickens with severe tumors, was the first reported recombinant MDV field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. GX0101 belongs to very virulent MDV (vvMDV) but has higher horizontal transmission ability than the vvMDV strain Md5. The complete genome sequence of GX0101 is 178,101 nucleotides (nt) and contains only one REV-LTR insert at a site 267 nt upstream of the sorf2 gene. Moreover, GX0101 has 5 repeats of a 217-nt fragment in its terminal repeat short (TRS) region and 3 repeats in internal repeat short (IRS) region, compared to the other 10 strains with only 1 or 2 repeats in both TRS and IRS.
Complete Genome Sequence of Bp7, an Escherichia coli Bacteriophage with a Wide Host Range
Authors: Zhang Can , Liu Wenhua , Ren Huiying 
Published: November 2012

ABSTRACT

Chicken colibacillosis is caused by some pathogenic
Escherichia coli
strains. Thirty-five pathogenic antibiotic-resistant
E. coli
strains were used in the host range detection of bacteriophage Bp7. The phage showed a wide range of
E. coli
hosts (46%). The complete genome of bacteriophage Bp7 was sequenced, assembled, and analyzed. The results revealed a linear double-stranded DNA sequence of 168,066 bp harboring 791 open reading frames. The major findings from its annotation are described.
Complete Genome Sequence Analysis of Human Echovirus Type 30 Isolated in China
Authors: Han Jian-Feng , Xu Li-Juan , Cao Rui-Yuan , Zhao Hui , Jiang Tao , Deng Yong-Qiang , Li Yue-Xiang , Zhu Shun-Ya , Yu Man , Qin E-De , Qin Cheng-Feng 
Published: November 2012

ABSTRACT
We report here the complete genome sequence of a human echovirus type 30 strain ECV30/GX10/05 isolated in Guangxi, China, in 2010. Phylogenetic analysis showed that ECV30/GX10/05 was closely related to a Korean strain isolated in 2008. The sequence information will help in an understanding of the molecular epidemiology and evolution of echovirus.
Complete Genome Sequence of the Novel Lytic Avian Pathogenic Coliphage NJ01
Authors: Li Yanzhe , Chen Mianmian , Tang Fang , Yao Huochun , Lu Chengping , Zhang Wei 
Published: November 2012

ABSTRACT

Bacteriophages of the C3 morphotype, characterized by very long heads that exceed their width several times, are extremely rare among the
Podoviridae
family members and constitute only 0.5% of over 5,500 phages that have been examined by the electron microscope (H. W. Ackermann, Arch. Virol. 152:227-243, 2007; H. W. Ackermann, Arch. Virol. 146:843-857, 2001). To date, among those phages proven to be C3, only coliphage phiEco32,
Lactococcus
phage KSY1,
Vibrio
phage 71A-6, and
Salmonella enterica
phage 7-11, but no avian pathogenic
Escherichia coli
(APEC) bacteriophages, have been completely sequenced (A. Chopin, H. Deveau, S. D. Ehrlich, S. Moineau, and M. C. Chopin, Virology 365:1-9, 2007; S. A. Khan, et al., Mol. Cell Probes 15:61-69, 2001; A. M. Kropinski, E. J. Lingohr, H. W. Ackermann, Arch. Virol. 156:149-151, 2011; D. Savalia, et al., J. Mol. Biol. 377:774-789, 2008) and are available in public databases. We isolated a bacteriophage from a scale duck market in Nanjing, Jiangsu province, named NJ01, that infects APEC. Sequence and morphological analyses revealed that phage NJ01 is a C3-like bacteriophage and belongs to the
Podoviridae
family. Here, we announce the complete genome sequence of phage NJ01 and submit the results of our analysis.
Complete Genome Sequence of the Bacteriophage YMC01/01/P52 PAE BP, Which Causes Lysis of Verona Integron-Encoded Metallo-β-Lactamase-Producing, Carbapenem-Resistant Pseudomonas aeruginosa
Authors: Jeon Jongsoo , Kim Jae-won , Yong Dongeun , Lee Kyungwon , Chong Yunsop 
Published: November 2012

ABSTRACT

Multidrug-resistant
Pseudomonas aeruginosa
commonly causes serious nosocomial infections. In this study, a novel lytic bacteriophage belonging to a member of the family
Podoviridae
, YMC01/01/P52 PAE BP, which infects carbapenem-resistant
Pseudomonas aeruginosa
, was isolated and characterized. YMC01/01/P52 PAE BP genome was analyzed by whole-genome sequencing and putative function identification. The bacteriophage genome consists of a double-stranded linear DNA genome of 49,381 bp with a GC content of 62.16%.
Managing the dyspnoeic emergency Patient
Authors: Firth Ava , Boag Amanda 
Published: November 2012

Correct and rapid management of respiratory emergencies is demanded of almost every veterinary practitioner. A structured, logical approach tothe dyspnoeic patient will maximise the chance of a successful outcome. This article looks at initial treatment of respiratory emergencies as well as respiratory diseases of the lower and upper airway and thoracic cage.
Precise QCD predictions on top quark pair production mediated by massive color-octet vector boson at hadron colliders
Authors: Zhu Hua Xing , Li Chong Sheng , Shao Ding Yu , Wang Jian , Yuan C.-P. 
Published: November 2012

AbstractWe present a theoretical framework for systematically calculating next-to-leading order (NLO) QCD effects to various experimental observables in models with massive COVB in a model independent way at hadron colliders. Specifically, we show the numerical results for the NLO QCD corrections to total cross sections, invariant mass distribution and AFB of top quark pairs production mediated by a massive COVB in both the fixed scale (top quark mass) scheme and the dynamical scale (top pair invariant mass) scheme. Our results show that the NLO QCD calculations in the dynamical scale scheme is more reasonable than the fixed scheme and the naive estimate of the NLO effects by simple rescaling of the LO results with the SM NLO K-factor is not appropriate.
Semantic and Perceptual Processing of Number Symbols: Evidence from a Cross-linguistic fMRI Adaptation Study
Authors: Holloway Ian D. , Battista Christian , Vogel Stephan E. , Ansari Daniel 
Published: November 2012

Abstract
The ability to process the numerical magnitude of sets of items has been characterized in many animal species. Neuroimaging data have associated this ability to represent nonsymbolic numerical magnitudes (e.g., arrays of dots) with activity in the bilateral parietal lobes. Yet the quantitative abilities of humans are not limited to processing the numerical magnitude of nonsymbolic sets. Humans have used this quantitative sense as the foundation for symbolic systems for the representation of numerical magnitude. Although numerical symbol use is widespread in human cultures, the brain regions involved in processing of numerical symbols are just beginning to be understood. Here, we investigated the brain regions underlying the semantic and perceptual processing of numerical symbols. Specifically, we used an fMRI adaptation paradigm to examine the neural response to Hindu-Arabic numerals and Chinese numerical ideographs in a group of Chinese readers who could read both symbol types and a control group who could read only the numerals. Across groups, the Hindu-Arabic numerals exhibited ratio-dependent modulation in the left IPS. In contrast, numerical ideographs were associated with activation in the right IPS, exclusively in the Chinese readers. Furthermore, processing of the visual similarity of both digits and ideographs was associated with activation of the left fusiform gyrus. Using culture as an independent variable, we provide clear evidence for differences in the brain regions associated with the semantic and perceptual processing of numerical symbols. Additionally, we reveal a striking difference in the laterality of parietal activation between the semantic processing of the two symbols types.
Interacting Cortical and Basal Ganglia Networks Underlying Finding and Tapping to the Musical Beat
Authors: Kung Shu-Jen , Chen Joyce L. , Zatorre Robert J. , Penhune Virginia B. 
Published: November 2012

Abstract
Humans are able to find and tap to the beat of musical rhythms varying in complexity from children's songs to modern jazz. Musical beat has no one-to-one relationship with auditory features—it is an abstract perceptual representation that emerges from the interaction between sensory cues and higher-level cognitive organization. Previous investigations have examined the neural basis of beat processing but have not tested the core phenomenon of finding and tapping to the musical beat. To test this, we used fMRI and had musicians find and tap to the beat of rhythms that varied from metrically simple to metrically complex—thus from a strong to a weak beat. Unlike most previous studies, we measured beat tapping performance during scanning and controlled for possible effects of scanner noise on beat perception. Results showed that beat finding and tapping recruited largely overlapping brain regions, including the superior temporal gyrus (STG), premotor cortex, and ventrolateral PFC (VLPFC). Beat tapping activity in STG and VLPFC was correlated with both perception and performance, suggesting that they are important for retrieving, selecting, and maintaining the musical beat. In contrast BG activity was similar in all conditions and was not correlated with either perception or production, suggesting that it may be involved in detecting auditory temporal regularity or in associating auditory stimuli with a motor response. Importantly, functional connectivity analyses showed that these systems interact, indicating that more basic sensorimotor mechanisms instantiated in the BG work in tandem with higher-order cognitive mechanisms in PFC.
The effects of acute hypoglycaemia on cognitive function in type 1 diabetes
Authors: Inkster Berit , Frier Brian M 
Published: November 2012

Throughout life with type 1 diabetes mellitus people with the condition are exposed to multiple episodes of hypoglycaemia associated with insulin therapy. Hypoglycaemia affects several domains of cognitive function. Studies in non-diabetic adults and in people with type 1 diabetes have shown that almost all domains of cognitive function are impaired to some degree during acute hypoglycaemia, with complex tasks being more greatly affected. The specific cognitive functions of attention and memory are both profoundly impaired during hypoglycaemia. These cognitive processes are fundamental to the performance of many day to day tasks. Their impairment disrupts everyday life and raises safety concerns for the pursuit of activities such as driving. Mood and emotion are also negatively affected by hypoglycaemia, resulting in tense tiredness, while motivation is reduced, and anger may be generated in some individuals. Hypoglycaemia can cause embarrassing social situations, and may lead to chronic anxiety and depression in people with type 1 diabetes. At present few therapeutic measures can modify or ameliorate the effects of hypoglycaemia on cognitive function, so instigation of measures to prevent exposure to hypoglycaemia is of major clinical importance, while preserving good glycaemic control.
Multiple graph regularized protein domain ranking
Authors: Wang Jim Jing-Yan , Bensmail Halima , Gao Xin 
Published: November 2012

Abstract

Background
Protein domain ranking is a fundamental task in structural biology. Most protein domain ranking methods rely on the pairwise comparison of protein domains while neglecting the global manifold structure of the protein domain database. Recently, graph regularized ranking that exploits the global structure of the graph defined by the pairwise similarities has been proposed. However, the existing graph regularized ranking methods are very sensitive to the choice of the graph model and parameters, and this remains a difficult problem for most of the protein domain ranking methods.

Results
To tackle this problem, we have developed the Multiple Graph regularized Ranking algorithm, MultiG-Rank. Instead of using a single graph to regularize the ranking scores, MultiG-Rank approximates the intrinsic manifold of protein domain distribution by combining multiple initial graphs for the regularization. Graph weights are learned with ranking scores jointly and automatically, by alternately minimizing an objective function in an iterative algorithm. Experimental results on a subset of the ASTRAL SCOP protein domain database demonstrate that MultiG-Rank achieves a better ranking performance than single graph regularized ranking methods and pairwise similarity based ranking methods.

Conclusion
The problem of graph model and parameter selection in graph regularized protein domain ranking can be solved effectively by combining multiple graphs. This aspect of generalization introduces a new frontier in applying multiple graphs to solving protein domain ranking applications.
CYP1A2 rs762551 polymorphism contributes to cancer susceptibility: a meta-analysis from 19 case-control studies
Authors: Wang Hongge , Zhang Zhi , Han Sugui , Lu Yujuan , Feng Fumin , Yuan Juxiang 
Published: November 2012

Abstract

Background
Genetic polymorphism (rs762551A>C) in gene encoding cytochrome P450 1A2 (CYP1A2) has been shown to influence the inducibility of CYP1A2 expression and thus might be associated with risk of several types of human cancer. However, the results of previous studies on the associations of this polymorphism with risk of cancer are not all consistent. To clarify the potential contribution of CYP1A2 rs762551 to cancer risk, we performed a meta-analysis of the published case–control studies.

Methods
We used PubMed, Embase, OVID, ScienceDirect, and Chinese National Knowledge Infrastructure databases to identify the related publications for this meta-analysis. The pooled odds ratio (OR) and 95% confidence interval (CI) were calculated using random effect model to evaluate the association of rs762551 with cancer risk. A χ
2-based Q-test was used to examine the heterogeneity assumption and the funnel plot and Egger’s test were used to examine the potential publication bias. The leave-one-out sensitivity analysis was conducted to determine whether our assumptions or decisions have a major effect on the results of the review.

Results
Our analysis of 19 eligible case–control studies showed a significant association between rs762551C variant with risk of cancer in the genetic model of CC versus AA (OR = 1.30, 95% CI = 1.02-1.64) and the dominant model (OR = 1.19, 95% CI = 1.04-1.36). In subgroup analysis based on ethnicity, the rs762551CC genotype was associated with increased cancer risk (OR = 1.29, 95% CI = 1.27-1.63 in co-dominate model and OR = 1.17, 95% CI = 1.02-1.34 in dominant model in Caucasians, but not in Asians and the mixed population.

Conclusion
These results suggested that CYP1A2 rs762551 polymorphism is likely to be associated with susceptibility to cancer in Caucasians.
Effect of gender on the association between weight status and health-related quality of life in adolescents
Authors: Bonsergent Emilie , Benie-Bi Joseph , Baumann Cédric , Agrinier Nelly , Tessier Sabrina , Thilly Nathalie , Briançon Serge 
Published: November 2012

AbstractBackgroundSome studies have investigated the association between body mass index (BMI) and health-related quality of life (HRQoL) among adolescents, but their results have been discrepant and few paid attention to the role of gender. The present investigation aimed to assess the relationship between weight status and HRQoL in adolescents and to verify whether it was similar in boys and girls.MethodsFive thousand two hundred and twenty six adolescents aged 14 to 18 years were included in the PRomotion de l’ALIMentation et de l’Activité Physique (PRALIMAP) trial, a 2x2x2 factorial cluster randomized trial performed in 24 high schools in France. Sociodemographic, anthropometric and HRQoL data were collected. BMI was categorized in four classes (thin, normal-weight, overweight, obese). Linear regression models were used to estimate the association between weight status and HRQoL, adjusting for confounders.ResultsThe mean age of adolescents was 15.7±0.6 years and their mean BMI was 21.6 ±3.5 kg/m2; 55% were girls. Boys were more often overweight and obese than were girls (overweight: 15.6% vs 14.2%, obese: 4.8% vs 3.3%), and girls were more likely to be thin (5.5% vs 4.5%, p=0.0042). All HRQoL scores were higher for boys (p=<0.0001). Weight status was not associated with physical and social scores neither in boys nor in girls. Conversely, it was associated with mental score, but differently in girls than boys. As compared with normal-weight girls, thin girls had better mental HRQoL (β=+6.17, p=0.0010), and overweight and obese girls had lower mental HRQoL (β=−3.89 and β=−5.90, respectively, p<0.001). Mental HRQoL was lower for thin, overweight and obese boys than for normal-weight boys (β= −4.97, β= −1.68 and β= −3.17, respectively, p<0.0001).ConclusionsGender can modify the association between weight status and HRQoL in adolescents. Body image could be an important target of public health programs to improve subjective health during adolescence.
Flaxseed oil and α-lipoic acid combination reduces atherosclerosis risk factors in rats fed a high-fat diet
Authors: Xu Jiqu , Yang Wei , Deng Qianchun , Huang Qingde , Yang Jin’e , Huang Fenghong 
Published: November 2012

Abstract

Background
Atherosclerosis is a major manifestation of the pathophysiology underlying cardiovascular disease. Flaxseed oil (FO) and α-lipoic acid (LA) have been reported to exert potential benefit to cardiovascular system. This study tried to assess the effect of supplement of FO and LA combination on the atherosclerosis risk factors in rats fed a high-fat diet.

Methods
LA was dissolved in flaxseed oil to a final concentration of 8 g/kg (FO+LA) when used. The rodent diet contained 20% fat. One-fifth of the fat was soybean oil and the others were lard (HFD group), or 75% lard and 25% FO+LA (L-FO+LA group), or 50% lard and 50% FO+LA (M-FO+LA group), or FO+LA (H-FO+LA group). Animals were fed for 10 weeks and then killed for blood collection.

Results
Supplement of FO and LA combination significantly enhanced plasma antioxidant defense capacities, as evaluated by the marked increase in the activities of SOD, CAT and GPx as well as the level of GSH, and the significant reduction in lipid peroxidation. Simultaneous intake of FO and LA also reduced plasma TG, TC and LDL-C contents and elevated the ratio of HDL-C/LDL-C. Besides, in parallel with the increase of FO and LA combination, plasma IL-6 and CRP levels were remarkably reduced.

Conclusion
Supplement of FO and LA combination may contribute to prevent atherogenesis by improving plasma oxidative stress, lipid profile and inflammation.