bluuebunny/crossref_metadata_2025_split huggingface dataset:
An organic acid-tolerant HAA1-overexpression mutant of an industrial bioethanol strain of Saccharomyces cerevisiae and its application to the production of bioethanol from sugarcane molasses
Authors: Inaba Takuya , Watanabe Daisuke , Yoshiyama Yoko , Tanaka Koichi , Ogawa Jun , Takagi Hiroshi , Shimoi Hitoshi , Shima Jun 
Published: December 2013

Abstract
Bacterial contamination is known as a major cause of the reduction in ethanol yield during bioethanol production by Saccharomyces cerevisiae. Acetate is an effective agent for the prevention of bacterial contamination, but it negatively affects the fermentation ability of S. cerevisiae. We have proposed that the combined use of organic acids including acetate and lactate and yeast strains tolerant to organic acids may be effective for the elimination of principally lactic acid bacterial (LAB) contamination. In a previous study employing laboratory S. cerevisiae strains, we showed that overexpression of the HAA1 gene, which encodes a transcriptional activator, could be a useful molecular breeding method for acetate-tolerant yeast strains. In the present study, we constructed a HAA1-overexpressing diploid strain (MAT
a/α, named ER HAA1-OP) derived from the industrial bioethanol strain Ethanol Red (ER). ER HAA1-OP showed tolerance not only to acetate but also to lactate, and this tolerance was dependent on the increased expression of HAA1 gene. The ethanol production ability of ER HAA1-OP was almost equivalent to that of the parent strain during the bioethanol production process from sugarcane molasses in the absence of acetate. The addition of acetate at 0.5% (w/v, pH 4.5) inhibited the fermentation ability of the parent strain, but such an inhibition was not observed in the ethanol production process using ER HAA1-OP.
HMGB1 is an early and critical mediator in an animal model of uveitis induced by IRBP-specific T cells
Authors: Jiang Guomin , Sun Deming , Yang Huan , Lu Qingxian , Kaplan Henry J , Shao Hui 
Published: December 2013

AbstractIt is largely unknown how invading autoreactive T cells initiate the pathogenic process inside the diseased organ in organ-specific autoimmune disease. In this study, we used a chronic uveitis disease model in mice—EAU—induced by adoptive transfer of uveitogenic IRBP-specific T cells and showed that HMGB1, an important endogenous molecule that serves as a danger signal, was released rapidly from retinal cells into the ECM and intraocular fluid in response to IRBP-specific T cell transfer. HMGB1 release required direct cell–cell contact between retinal cells and IRBP-specific T cells and was an active secretion from intact retinal cells. Administration of HMGB1 antagonists inhibited severity of EAU significantly via mechanisms that include inhibition of IRBP-specific T cell proliferation and their IFN-γ and IL-17 production. The inflammatory effects of HMGB1 may signal the TLR/MyD88 pathway, as MyD88−/− mice had a high level of HMGB1 in the eye but did not develop EAU after IRBP-specific T cell transfer. Our study demonstrates that HMGB1 is an early and critical mediator of ocular inflammation initiated by autoreactive T cell invasion.
Multiwell Thermal Interaction: Field-Data Validation of Transient Model for Closely Spaced Wells
Authors: McSpadden Albert R. , Gunn Alex , Dunagan Craig 
Published: December 2013

Summary
Wellbore-temperature logs and associated field-history data from a high-pressure/high-temperature (HP/HT) condensate North Sea platform are presented that validate the accuracy of a transient model of the multiwell thermal interaction (MWTI). The model is an updated version of previous work by McSpadden and Coker (2010) that simulates transient thermal interaction or "cross heating" between closely spaced wells of a template. As discussed in the preceding work, the MWTI alters final wellbore temperatures as well as formation temperatures in the interwell zone and also farther out from the well template. The multiwell thermal model is shown to converge closely in very characteristic fashion to two different logged and measured temperature profiles at a vertical depth range of more than 3,000 ft.
The empirical data, including field history, represent a unique opportunity to study and understand this important topic. Before this current work, the authors’ experience found the industry discussion of the MWTI or "cross heating" to be largely anecdotal. Model validation against field data is necessary to achieve a full understanding of the physical system and to provide confidence in the predictive capability.
The modeling of wellbore and formation temperatures for closely spaced wells has not been widely examined in the industry literature, as observed by Bellarby (2009). The current work presents an improved methodology on the basis of standard-industry techniques. The method uses standard industry thermal/hydraulic modeling software for a single well and a fully transient finite-difference model for the formation in a loosely coupled, iterative analysis. The iteration scheme is achieved by the coupling of the standard analytical solution for the isolated single-well temperature scenario with the solution in the formation for the cross-heating scenario.
The effect of the MWTI is important for closely spaced wells such as offshore platforms or subsea and Arctic developments. The multiwell disturbance on formation and wellbore temperatures may affect well design, facilities planning, and operations. For example, given nominal flowing wellhead temperatures (FWHTs) approaching 350°F for HP/HT platform developments, even small temperature increases may have a critical impact on the design and layout of surface receiving systems. Annular-pressure buildup (APB), wellhead movement, tubular-stress design, cement-slurry design, subsidence/compaction effects, and facilities health and safety issues can all be affected. If the MWTI is not taken into account, then load events such as APB, wellhead movement, and thermally induced stresses may be underestimated. Concurrent and batch-drilling operations, including cementation, will also be affected.
Prime, irreducible elements and coatoms in posets
Authors: Zhou Weifeng , Li Qingguo , Guo Lankun 
Published: December 2013

Abstract
In this paper, some properties of prime elements, pseudoprime elements, irreducible elements and coatoms in posets are investigated. We show that the four kinds of elements are equivalent to each other in finite Boolean posets. Furthermore, we demonstrate that every element of a finite Boolean poset can be represented by one kind of them. The example presented in this paper indicates that this result may not hold in every finite poset, but all the irreducible elements are proved to be contained in each order generating set. Finally, the multiplicative auxiliary relation on posets and the notion of arithmetic poset are introduced, and some properties about them are generalized to posets.
Rational permutation groups containing a full cycle
Authors: Erkoç Temha , Yilmaztürk Utku 
Published: December 2013

Abstract
A finite group whose irreducible complex characters are rational valued is called a rational group. Thus, G is a rational group if and only if N
G(〈x〉)/C
G(〈x〉) ≌ Aut(〈x〉) for every x ∈ G. For example, all symmetric groups and their Sylow 2-subgroups are rational groups. Structure of rational groups have been studied extensively, but the general classification of rational groups has not been able to be done up to now. In this paper, we show that a full symmetric group of prime degree does not have any rational transitive proper subgroup and that a rational doubly transitive permutation group containing a full cycle is the full symmetric group. We also obtain several results related to the study of rational groups.
q-subharmonicity and q-convex domains in ℂn
Authors: Khue Nguyen , Hai Le , Hong Nguyen 
Published: December 2013

Abstract
In this paper we study q-subharmonic and q-plurisubharmonic functions in ℂn. Next as an application, we give the notion of q-convex domains in ℂn which is an extension of weakly q-convex domains introduced and investigated in [10]. In the end of the paper we show that the q-convexity is the local property and give some examples about q-convex domains.
Existence and multiplicity of periodic solutions for some second order differential systems with p(t)-Laplacian
Authors: Zhang Liang , Tang X. 
Published: December 2013

Abstract
In this paper, we deal with the existence and multiplicity of periodic solutions for the p(t)-Laplacian Hamiltonian system. Some new existence theorems are obtained by using the least action principle and minmax methods in critical point theory, and our results generalize and improve some existence theorems.
Henstock-Kurzweil-Pettis integral and weak topologies in nonlinear integral equations on time scales
Authors: Satco Bianca-Renata , Turcu Corneliu-Octavian 
Published: December 2013

Abstract
The goal of the present work is to give an existence result for a nonlinear integral equation on time scales by considering the Banach space endowed with its weak topology. More precisely, we obtain the existence of weakly continuous solutions for an integral equation that has on the right hand side the sum of two operators, one of them continuous while the other one satisfies a partial continuity condition and some integrability (in a nonabsolute sense) assumptions.
Gruppen secret sharing or how to share several secrets if you must?
Authors: Csirmaz László 
Published: December 2013

Abstract
Each member of an n-person team has a secret, say a password. The k out of n gruppen secret sharing requires that any group of k members should be able to recover the secrets of the other n − k members, while any group of k − 1 or less members should have no information on the secret of other team member even if other secrets leak out. We prove that when all secrets are chosen independently and have size s, then each team member must have a share of size at least (n − k)s, and we present a scheme which achieves this bound when s is large enough. This result shows a significant saving over n independent applications of Shamir’s k out of n − 1 threshold schemes which assigns shares of size (n − 1)s to each team member independently of k.
We also show how to set up such a scheme without any trusted dealer, and how the secrets can be recovered, possibly multiple times, without leaking information. We also discuss how our scheme fits to the much-investigated multiple secret sharing methods.
On ΛIs-sets and the related notions in ideal topological spaces
Authors: Sanabria José , Rosas Ennis , Carpintero Carlos 
Published: December 2013

Abstract
In this paper, we define and study the notions of ΛIs-sets, ΛIs-closed sets and I-generalized semi-closed (briefly I-gs-closed) sets by using semi-I-open sets in an ideal topological space. Moreover, we present and characterize two new low separation axioms using the above notions.
Phylogenetics and diversification of morning glories (tribe Ipomoeeae, Convolvulaceae) based on whole plastome sequences
Authors: Eserman Lauren A. , Tiley George P. , Jarret Robert L. , Leebens‐Mack Jim H. , Miller Richard E. 
Published: December 2013

• Premise of the study: Morning glories are an emerging model system, and resolving phylogenetic relationships is critical for understanding their evolution. Phylogenetic studies demonstrated that the largest morning glory genus, Ipomoea, is not monophyletic, and nine other genera are derived from within Ipomoea. Therefore, systematic research is focused on the monophyletic tribe Ipomoeeae (ca. 650–900 species). We used whole plastomes to infer relationships across Ipomoeeae.• Methods: Whole plastomes were sequenced for 29 morning glory species, representing major lineages. Phylogenies were estimated using alignments of 82 plastid genes and whole plastomes. Divergence times were estimated using three fossil calibration points. Finally, evolution of root architecture, flower color, and ergot alkaloid presence was examined.• Key results: Phylogenies estimated from both data sets had nearly identical topologies. Phylogenetic results are generally consistent with prior phylogenetic hypotheses. Higher‐level relationships with weak support in previous studies were recovered here with strong support. Molecular dating analysis suggests a late Eocene divergence time for the Ipomoeeae. The two clades within the tribe, Argyreiinae and Astripomoeinae, diversified at similar times. Reconstructed most recent common ancestor of the Ipomoeeae had blue flowers, an association with ergot‐producing fungi, and either tuberous or fibrous roots.• Conclusions: Phylogenetic results provide confidence in relationships among Ipomoeeae lineages. Divergence time estimation results provide a temporal context for diversification of morning glories. Ancestral character reconstructions support previous findings that morning glory morphology is evolutionarily labile. Taken together, our study provides strong resolution of the morning glory phylogeny, which is broadly applicable to the evolution and ecology of these fascinating species.
Listening With their Eyes: Problems for Radical Intertextuality
Authors: Gracyk Theodore 
Published: December 2013

Tout produit culturel implique des prédécesseurs et des influences, et la musique populaire ne déroge pas à cette règle. Dans le cas du rock, le sens d’un disque ou d’une chanson ne dépend donc pas moins de l’intertextualité que dans celui de tout autre cas de communication signifiante. Cet article soutient qu’il faut refuser la position radicale selon laquelle tout lien intertextuel est d’égale importance. Si cela était vrai, alors l’expérience consistant à écouter de la musique n’aurait plus qu’une importance limitée pour en déterminer la signification. L’auteur défend une version moins radicale de l’intertextualité : prenant le rock comme exemple de musique populaire, il montre que cette version moins radicale rend mieux compte de la raison pour laquelle les amateurs de rock écoutent cette musique.
« Écouter avec les yeux » : sur quelques problèmes de l’intertextualité radicale
Authors: Gracyk Theodore 
Published: December 2013

Tout produit culturel implique des prédécesseurs et des influences, et la musique populaire ne déroge pas à cette règle. Dans le cas du rock, le sens d’un disque ou d’une chanson ne dépend donc pas moins de l’intertextualité que dans celui de tout autre cas de communication signifiante. Cet article soutient qu’il faut refuser la position radicale selon laquelle tout lien intertextuel est d’égale importance. Si cela était vrai, alors l’expérience consistant à écouter de la musique n’aurait plus qu’une importance limitée pour en déterminer la signification. L’auteur défend une version moins radicale de l’intertextualité : prenant le rock comme exemple de musique populaire, il montre que cette version moins radicale rend mieux compte de la raison pour laquelle les amateurs de rock écoutent cette musique.
Premature Terminal Differentiation Protects from Deregulated Lymphocyte Activation by ITK-Syk
Authors: Bach Martina P. , Hug Eva , Werner Markus , Holch Julian , Sprissler Clara , Pechloff Konstanze , Zirlik Katja , Zeiser Robert , Dierks Christine , Ruland Jürgen , Jumaa Hassan 
Published: December 2013

Abstract
The development of hematopoietic neoplasms is often associated with mutations, altered gene expression or chromosomal translocations. Recently, the t(5, 9)(q33;q22) translocation was found in a subset of peripheral T cell lymphomas and was shown to result in an IL-2–inducible kinase–spleen tyrosine kinase (ITK-Syk) fusion transcript. In this study, we show that T cell–specific expression of the ITK-Syk oncogene in mice leads to an early onset and aggressive polyclonal T cell lymphoproliferation with concomitant B cell expansion and systemic inflammation by 7–9 wk of age. Because this phenotype is strikingly different from previous work showing that ITK-Syk expression causes clonal T cell lymphoma by 20–27 wk of age, we investigated the underlying molecular mechanism in more detail. We show that the reason for the severe phenotype is the lack of B-lymphocyte–induced maturation protein-1 (Blimp-1) induction by low ITK-Syk expression. In contrast, high ITK-Syk oncogene expression induces terminal T cell differentiation in the thymus by activating Blimp-1, thereby leading to elimination of oncogene-expressing cells early in development. Our data suggest that terminal differentiation is an important mechanism to prevent oncogene-expressing cells from malignant transformation, as high ITK-Syk oncogene activity induces cell elimination. Accordingly, for transformation, a specific amount of oncogene is required, or alternatively, the induction of terminal differentiation is defective.
Myeloid Cells Limit Production of Antibody-Secreting Cells after Immunization in the Lymph Node
Authors: Fooksman David R. , Nussenzweig Michel C. , Dustin Michael L. 
Published: December 2013

Abstract
Ab-secreting cell (ASC) expansion and survival are important processes in optimizing vaccines and controlling autoimmunity. The microenvironment of the medullary cords is positioned to control these key processes. Previously, we imaged and characterized ASC differentiation and migration by intravital microscopy in the lymph node (LN) by transferring and activating B cells expressing yellow fluorescent protein only in the ASC compartment. In this study, we observed that yellow fluorescent protein+ ASCs in the medullary cords migrated along myelomonocytic cells and arrested in contact with them. Acute ablation of myeloid cells using the human diphtheria receptor system (diphtheria toxin receptor [DTR]) expressed in Lysmd1-cre–positive cells increased ASC and Ab production by 2-fold. Increases in ASC numbers were associated with cell proliferation based on Ki-67 staining, rather than reduced apoptosis, or changes in egress from the LN. Using DTR-mediated ablation targeted to Ccr2-expressing myeloid cells also generated increases in ASCs. In contrast, neither the depletion of Gr-1–positive cells with an Ab nor the ablation of cells using a cd11c-DTR resulted in any change in ASCs. IL-6 cytokine signaling can enhance ASC production and has been implicated in dampening ASCs in lupus mouse models through myeloid cells. Using mixed bone marrow chimeras, we observed that IL-6 enhances ASC production, but IL-6 production was not required by myeloid cells to dampen ASCs in the LN. Inhibition of ASCs by these myeloid cells in the LN provides a new regulatory mechanism with implications for tuning Ab responses.
Integrating B Cell Lineage Information into Statistical Tests for Detecting Selection in Ig Sequences
Authors: Uduman Mohamed , Shlomchik Mark J. , Vigneault Francois , Church George M. , Kleinstein Steven H. 
Published: December 2013

Abstract
Detecting selection in B cell Ig sequences is critical to understanding affinity maturation and can provide insights into Ag-driven selection in normal and pathologic immune responses. The most common sequence-based methods for detecting selection analyze the ratio of replacement and silent mutations using a binomial statistical analysis. However, these approaches have been criticized for low sensitivity. An alternative method is based on the analysis of lineage trees constructed from sets of clonally related Ig sequences. Several tree shape measures have been proposed as indicators of selection that can be statistically compared across cohorts. However, we show that tree shape analysis is confounded by underlying experimental factors that are difficult to control for in practice, including the sequencing depth and number of generations in each clone. Thus, although lineage tree shapes may reflect selection, their analysis alone is an unreliable measure of in vivo selection. To usefully capture the information provided by lineage trees, we propose a new method that applies the binomial statistical framework to mutations identified based on lineage tree structure. This hybrid method is able to detect selection with increased sensitivity in both simulated and experimental data sets. We anticipate that this approach will be especially useful in the analysis of large-scale Ig sequencing data sets generated by high-throughput sequencing technologies.
Follicular B Cells in Thyroids of Mice with Spontaneous Autoimmune Thyroiditis Contribute to Disease Pathogenesis and Are Targets of Anti-CD20 Antibody Therapy
Authors: Hong So-Hee , Braley-Mullen Helen 
Published: December 2013

Abstract
B cells are required for development of spontaneous autoimmune thyroiditis (SAT) in NOD.H-2h4 mice where they function as important APCs for activation of CD4+ T cells. Depletion of B cells using anti-CD20 effectively inhibits SAT development. The goals of this study were to characterize the B cells that migrate to thyroids in SAT, and to determine whether anti-CD20 effectively targets those B cells in mice with established SAT. The results showed that most thyroid-infiltrating B cells in mice with SAT are follicular (FO) B cells. Expression of CD80, CD86, and CD40 was significantly increased on FO, but not marginal zone, splenic B cells after SAT development. Thyroid-infiltrating and peripheral blood B cells had lower expresion of CD20 and CD24 compared with splenic and lymph node FO B cells. Despite reduced CD20 expression, anti-CD20 depleted most B cells in thyroids of mice with established SAT within 3 d. B cell depletion in thyroids of mice given anti-CD20 was more complete and longer lasting than in spleen and lymph nodes and was comparable to that in blood. Circulation of B cells was required for effective and rapid removal of B cells in thyroids because preventing lymphocyte egress by administration of FTY720 abrogated the effects of anti-CD20 on thyroid B cells. Therefore, the FO subset of B cells preferentially contributes to SAT development and persistence, and anti-CD20 targeting of FO B cells effectively eliminates B cells in the target organ even though thyroid B cells have decreased CD20 expression.