bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bilateral Subthalamic Stimulation can Improve Sleep Quality in Parkinson’s Disease
Authors: Deli Gabriella , Aschermann Zsuzsanna , Ács Péter , Bosnyák Edit , Janszky József , Faludi Béla , Makkos Attila , Kovács Márton , Komoly Sámuel , Balás István , Dóczi Tamás , Kovács Norbert 
Published: June 2015

Background: Sleep problems are among the most common non-motor symptoms of Parkinson’s disease (PD). The PD Sleep Scale 2nd version (PDSS-2) improved the original PDSS by adding more items on different aspects of sleep problems, making it a more robust tool to evaluate the severity of sleep disturbances. However, previous studies on deep brain stimulation (DBS) have not used the PDSS-2. Objective: To determine if the PDSS-2 could detect improvement reliably in sleep problems after bilateral subthalamic nucleus DBS for PD. Methods: In this prospective study, 25 consecutive patients undergoing DBS implantation were enrolled. Patients were examined twice: 1 week prior to the DBS implantation (baseline) and 12 months postoperatively. Severity of PD symptoms were assessed by the Movement Disorders Society Unified PD Rating Scale (MDS-UPDRS) and the Non-Motor Symptoms Scale (NMSS). Presence and severity of sleep disturbances were specifically measured by PDSS-2. Results: Total score of MDS-UPDRS improved from 81 (median, interquartile-range: 63-103) to 55 points (median, IQR: 46-75, p <  0.001). Health-related quality of life, measured by PDQ-39, also improved from 29 (IQR: 18-40) to 15 (IQR: 9-28) points ( p = 0.002). Most domains of NMSS also improved. At baseline 13 patients reported sleep problems, but 1 year after DBS implantation only 3 did ( p = 0.012). Although only 6 out of 15 items showed a significant decrease after DBS implantation, the total score of PDSS-2 decreased from 24 (IQR: 17-32) to 10 (IQR: 7-18) points ( P <  0.001). Conclusions: Based on our results, PDSS-2 can detect improvements in sleep quality reliably after DBS implantation.
Grouping of Pseudomonas spp. isolated from dieback- affected sissoo (Dalbergia sissoo Roxb.) using phylogenetic analyses
Authors: Mühlbach HP , Tantau H , Stubbe A , Palm D , Schulze J , Hoque MI , Sarker RH , Alam Sk S , Saha ML , Khan Md S 
Published: June 2015

Dieback of sissoo (Dalbergia sissoo Roxb.) is a devastating plant disease, whose causative biotic agents were not yet identified unequivocally. Our previous studies revealed that bacteria belonging to the genus Pseudomonas were associated with dieback?affected sissoo trees. To study the bacterial community associated with dieback-affected sissoo trees, DNA based approaches were used in the present work. Sequencing almost the complete 16S rRNA gene of 33 selected Pseudomonas isolates allowed them to be grouped into two main clusters only. The major group consisted of 19 isolates related to P. oryzihabitans, while in the minor one ten isolates related to P. putida were found together with very few other pseudomonads. Almost the same clustering was obtained a with the independent grouping methods of amplified ribosomal DNA restriction analysis (ARDRA). These results strongly indicate that mainly bacteria strains related to Pseudomonas oryzihabitans were associated with dieback-affected Dalbergia sissoo trees. Immuno-histological studies with thin-sections of sissoo roots using Pseudomonas-specific antibodies showed that bacteria had invaded the root parenchyma.
Plant Tissue Cult. & Biotech. 24(2): 141-153, 2014 (December)
In vitro micropropagation and cytomorphological evaluation of Centella asiatica (L.) urban (Mandukparni) from Himachal Pradesh, India - an endemic, endangered and threatened herb
Authors: Singh Gurwinder , Kaur Bhawandeep , Sharma Navdeep , Bano Anisha , Kumar Sanjeev , Dhaliwal Harcharan S , Sharma Vivek 
Published: June 2015

The present research work reports the cytomorphological evaluation and an efficient protocol for in vitro micropropagation of an endemic, endangered and threatened herb Centella asiatica (L.) Urban (Mandukparni) from Himachal Pradesh, India. Plant species is a potent memory enhancer, antidiabetic, antioxidant, antimutagenic, anticancerous and also reported to have cardiovascular properties and used to cure chronic hepatitis disorders. Eight morphometric characters for each 10 different accessions were extensively studied, but no new morphotype was reported. As per the cytology, the present report (2n = 18) confirmed the earlier chromosome counts from India and abroad. As there is an important need to preserve this plant species and to make it available all over the year to pharmaceutical industries without causing loss of germplasm from the wild region, efficient in vitro micropropagation protocol through nodal explant has been developed. As per the results, the highest percentage of multiple shoot induction was 90.20 showing average 16.3 number of shoots on the medium augmented with 2.0 mg/l BAP + 0.5 mg/l Kn. Whereas, the combined concentration of 1.0 mg/l NAA + 1.0 mg/l IBA showed highest 92.2% root induction with average 16.5 number of roots per shoot. The survival rate of these plantlets under green house condition was 80%. This protocol can be used for further regeneration and genetic transformation studies in Centella
Plant Tissue Cult. & Biotech. 24(2): 155-171, 2014 (December)
Effect of light emutting diode (LED) lamps and N-acetylglucosamine (NAG) on organogenesis in protocorm-like bodies (PLBs) of a Cymbidium hybrid cultured in vitro
Authors: Kamal Mohammad Mostafa , Shimasaki Kazuhiko , Akter Nasren 
Published: June 2015

Abstract not available
Plant Tissue Cult. & Biotech. 24(2): 273-277, 2014 (December)
The OMERACT First-time Participant (“Newbie”) Program: Initial Assessment and Lessons Learned
Authors: Sloan Victor S. , Grosskleg Shawna , Pohl Christoph , Wells George A. , Singh Jasvinder A. 
Published: June 2015

Objective.To describe the experience of a first-time participant (“newbie”) training program at the Outcome Measures in Rheumatology 12 meeting in 2014.Methods.We conducted newbie sessions at OMERACT 12, including a 2-hour introductory session on Day 1, followed by 1-h evening followup sessions on days 1–4 of OMERACT 12. Pre- and postmeeting surveys assessed participants’ level of comfort with the principles of the OMERACT Filters 1.0 (truth, discrimination, feasibility), and Filter 2.0 (the essential tools for OMERACT methodology), the different types of OMERACT sessions, and whether participants felt welcome.Results.In all, 25 new attendees participated in the introductory session and 10–16 attended followup sessions. Fewer participants reported being somewhat or extremely uncomfortable with the meeting, comparing Day 1 (preintroductory session) to days 1–4 (post): (1) with different OMERACT sessions: 56% (pre) versus 6%, 0%, 8%, and 6% (post days 1–4, respectively); and (2) with principles of the OMERACT filter, 64% (pre) versus 7%, 0%, 8%, and 0% (post), respectively. Most reported feeling welcome (100%) and that they were able to contribute substantively to breakout sessions (87%) on Day 1 evening; results were sustained on days 2–4.Conclusion.First-time participant training sessions increased the comfort level of the participants with the OMERACT meeting structure and filter, and increased the ability of the new attendees to feel they could contribute to the OMERACT process.
Relationship between Serum Magnesium Concentration and Radiographic Knee Osteoarthritis
Authors: Zeng Chao , Wei Jie , Li Hui , Yang Tuo , Zhang Fang-Jie , Pan Ding , Xiao Yong-Bing , Yang Tu-Bao , Lei Guang-Hua 
Published: June 2015

Objective.To establish whether there is a relationship between serum magnesium (Mg) concentration and radiographic knee osteoarthritis (OA).Methods.There were 2855 subjects in this cross-sectional study. Serum Mg concentration was measured using the chemiluminescence method. Radiographic OA of the knee was defined as changes consistent with Kellgren-Lawrence (K-L) grade 2 on at least 1 side. Mg concentration was classified into 1 of 4 quartiles: ≤ 0.87, 0.88–0.91, 0.92–0.96, or ≥ 0.97 mmol/l. Multivariable logistic analysis was used to test the association between serum Mg and radiographic knee OA after adjustment for potentially confounding factors. The OR with 95% CI for the association between radiographic knee OA and serum Mg concentration were calculated for each quartile. The quartile with the lowest value was regarded as the reference category.Results.Significant association between serum Mg concentration and radiographic knee OA was observed in the model after adjustment for age, sex, and body mass index, as well as in the multivariable model. The multivariable-adjusted OR (95% CI) for radiographic knee OA in the second, third, and fourth serum Mg concentration quartiles were 0.90 (95% CI 0.71–1.13), 0.92 (95% CI 0.73–1.16), and 0.72 (95% CI 0.57–0.92), respectively, compared with the lowest (first) quartile. A clear trend (p for trend was 0.01) was observed. The relative odds of radiographic knee OA was decreased by 0.72 times in the fourth serum Mg quartile compared with the lowest quartile.Conclusion.Serum Mg concentration may have an inverse relationship with radiographic OA of the knee.
Can the Cancer-related Fatigue Case-definition Criteria Be Applied to Chronic Medical Illness? A Comparison between Breast Cancer and Systemic Sclerosis
Authors: Kwakkenbos Linda , Minton Ollie , Stone Patrick C. , Alexander Susanna , Baron Murray , Hudson Marie , Thombs Brett D. ,   and the Canadian Scleroderma Research Group
Published: June 2015

Objective.Fatigue is a crucial determinant of quality of life across rheumatic diseases, but the lack of agreed-upon standards for identifying clinically significant fatigue hinders research and clinical management. Case definition criteria for cancer-related fatigue were proposed for inclusion in the International Classification of Diseases. The objective was to evaluate whether the cancer-related fatigue case definition performed equivalently in women with breast cancer and systemic sclerosis (SSc) and could be used to identify patients with chronic illness-related fatigue.Methods.The cancer-related fatigue interview (case definition criteria met if ≥ 5 of 9 fatigue-related symptoms present with functional impairment) was completed by 291 women with SSc and 278 women successfully treated for breast cancer. Differential item functioning was assessed with the multiple indicator multiple cause model.Results.Items 3 (concentration) and 10 (short-term memory) were endorsed significantly less often by women with SSc compared with cancer, controlling for responses on other items. Omitting these 2 items from the case definition and requiring 4 out of the 7 remaining symptoms resulted in a similar overall prevalence of cancer-related fatigue in the cancer sample compared with the original criteria (37.4% vs 37.8%, respectively), with 97.5% of patients diagnosed identically with both definitions. Prevalence of chronic illness-related fatigue was 36.1% in SSc using 4 of 7 symptoms.Conclusion.The cancer-related fatigue criteria can be used equivalently to identify patients with chronic illness-related fatigue when 2 cognitive fatigue symptoms are omitted. Harmonized definitions and measurement of clinically significant fatigue will advance research and clinical management of fatigue in rheumatic diseases and other conditions.
Evaluation of the Satisfaction with Appearance Scale and Its Short Form in Systemic Sclerosis: Analysis from the UCLA Scleroderma Quality of Life Study
Authors: Mills Sarah D. , Fox Rina S. , Merz Erin L. , Clements Philip J. , Kafaja Suzanne , Malcarne Vanessa L. , Furst Daniel E. , Khanna Dinesh 
Published: June 2015

Objective.Changes in appearance are common in patients with systemic sclerosis (SSc) and can significantly affect well-being. The Satisfaction with Appearance Scale (SWAP) measures body image dissatisfaction in persons with visible disfigurement; the Brief-Satisfaction with Appearance Scale (Brief-SWAP) is its short form. The present study evaluated the reliability and validity of SWAP and Brief-SWAP scores in SSc.Methods.A sample of 207 patients with SSc participating in the University of California, Los Angeles Scleroderma Quality of Life Study completed the SWAP. Brief-SWAP scores were derived from the SWAP. The structural validity of both measures was investigated using confirmatory factor analysis. Internal consistency reliability of total and subscale scores was assessed with Cronbach’s alpha coefficients. Convergent and divergent validity was evaluated using the Center for Epidemiological Studies Depression Scale, the Health Assessment Questionnaire-Disability Index, and the Medical Outcomes Study Short Form-36 questionnaire.Results.SWAP and Brief-SWAP total scores were highly correlated (r = 0.97). The 4-factor structure of the SWAP fit well descriptively; the 2-factor structure of the Brief-SWAP fit well descriptively and statistically. Internal consistencies for total and subscale scores were good, and results supported convergent and divergent validity.Conclusion.Both versions are suitable for use in patients with SSc. The Brief-SWAP is most efficient; the full SWAP yields additional subscales that may be informative in understanding body image issues in patients with SSc.
Treatment Patterns of Multimorbid Patients with Rheumatoid Arthritis: Results from an International Cross-sectional Study
Authors: Radner Helga , Yoshida Kazuki , Hmamouchi Ihsane , Dougados Maxime , Smolen Josef S. , Solomon Daniel H. 
Published: June 2015

Objective.To describe the treatment profile of multimorbid patients with rheumatoid arthritis (RA) in contrast to patients with RA only.Methods.COMORA (Comorbidities in Rheumatoid Arthritis) is a cross-sectional, international study assessing morbidities, outcomes, and treatment of patients with RA. Patients were grouped according to their multimorbidity profile assessed by a counted multimorbidity index (cMMI). Treatment for RA was categorized as use of biologic disease-modifying antirheumatic drugs (bDMARD), in particular tumor necrosis factor inhibitors (TNFi), synthetic DMARD (sDMARD) use only, nonsteroidal antiinflammatory drug (NSAID) use, and corticosteroid use. Logistic regression models were performed to determine the OR of bDMARD, TNFi, sDMARD, NSAID, or corticosteroid use based on a patient’s cMMI and global region after adjusting for age, disease activity, disease duration, educational level, and previous DMARD therapy.Results.Out of 3920 patients, 32.7% received bDMARD; 59.9% sDMARD only, 51.1% used concomitant NSAID, and 54.8% used corticosteroid. Regional differences were observed with the most frequent use of bDMARD in the United States (46.5%) and lowest in North Africa (9%). After adjusting for confounders in logistic regression, the OR for bDMARD use was reduced for each additional morbidity (OR 0.89, 95% CI 0.83–0.96). Similar results were found for TNFi (OR 0.91, 95% CI 0.84–0.99), whereas the OR for use of sDMARD was increased (1.13, 95% CI 1.05–1.22). No significant change of OR was found for NSAID or corticosteroid use.Conclusion.In this study, the odds of bDMARD use decreases 11% for each additional chronic morbid condition after adjustment for regional differences, disease activity, and other covariates.
Anakinra in Systemic Juvenile Idiopathic Arthritis: A Single-center Experience
Authors: Pardeo Manuela , Pires Marafon Denise , Insalaco Antonella , Bracaglia Claudia , Nicolai Rebecca , Messia Virginia , De Benedetti Fabrizio 
Published: June 2015

Objective.To assess anakinra as a therapy for systemic juvenile idiopathic arthritis (sJIA) in a single-center series.Methods.We reviewed 25 patients with sJIA treated with anakinra for at least 6 months. The primary outcome was the number of patients who achieved clinically inactive disease at 6 months, according to preliminary criteria for inactive disease and clinical remission of JIA.Results.Among 25 patients evaluated, 14 (56%) met the criteria for inactive disease at 6 months and were classified as responders. For each individual patient, we compared the dose administered with the ideal dose of anakinra and we found that there was no relation with response. We also compared demographic characteristics and clinical and laboratory features at baseline in responders and non-responders: no differences were observed in relation with the number of active joints before starting anakinra or concomitant glucocorticoids treatment. The only variable significantly associated with response was the time from disease onset to receiving anakinra, with earlier treatment being associated with a better outcome.Conclusion.Anakinra is associated with rapid attainment of inactive disease in a significant portion of patients. We found that only the earlier treatment is associated with better outcome. However, formal studies on early treatment and on the pathophysiology and response to treatments, including anakinra, of early- and late-onset sJIA are needed to optimize the management of this challenging disease.
Penser et parler. La transformation de la grammaire philosophique de Fichte à Humboldt
Authors: Thouard Denis 
Published: June 2015

Les thèses de 1795 indiquent précisément comment Humboldt comprend alors le rôle du langage dans le « travail de l’esprit » accédant à la conscience de soi. Ainsi, les coordonnées d’une réflexion adéquate sur le langage en général sont posées dès ces premières pages humboldtiennes. Sans reprendre le style de la spéculation fichtéenne, Humboldt retient cependant l’idée d’une indispensable réflexion générale sur le langage comme orientation de toute étude spécifique. Il fait porter sa réflexion sur le motif d’une libération par le langage, condition et amplification de la conscience de soi. En explicitant la fonction d’autoposition de la conscience de soi dans le langage, Humboldt place celui-ci au centre de la subjectivité. La fonction du verbe représentant l’acte synthétique de la conscience de soi demeurera le point crucial de son analyse des langues. Par son appropriation de Kant et de Fichte, Humboldt préparait le fondement de son analyse du langage ultérieure dans son aspect universel. Il la complètera par la dimension de la diversité sans jamais renier l’orientation philosophique.
Le questionnaire sur la famille : contribution anthropologique et pastorale
Authors: Defois Gérard 
Published: June 2015

Le synode sur la famille convoqué par le Pape François peut achopper sur deux illusions : celle de croire que le langage théologique traditionnel peut redresser la conscience commune du sacrement de mariage, celle de parler comme si notre discours faisait autorité par lui-même dans la culture contemporaine. Comme souvent, la manière dont nous abordons la question conditionne les réponses que nous apportons. Le débat d’aujourd’hui reflète les impacts d’une culture où les valeurs sont réinterprétées de façon « narcissique ». Comme tel le discours magistériel, fondé dans la vérité doctrinale que nous savons, est sommé de faire ses preuves en termes de désir et de bonheur. Ce qui ne peut être que le lieu d’un débat, tant d’éthique que de culture, non pour s’aligner sur les attentes, mais pour s’identifier dans ses transcendances.
The Importance of IL-6 in the Development of LAT-Mediated Autoimmunity
Authors: O’Brien Sarah A. , Zhu Minghua , Zhang Weiguo 
Published: June 2015

AbstractLinker for activation of T cells (LAT) is a transmembrane adaptor protein that is highly tyrosine phosphorylated upon engagement of the TCR. Phosphorylated LAT binds Grb2, Gads, and phospholipase C (PLC)γ1 to mediate T cell activation, proliferation, and cytokine production. T cells from mice harboring a mutation at the PLCγ1 binding site of LAT (Y136F) have impaired calcium flux and Erk activation. Interestingly, these T cells are highly activated, resulting in the development of a lymphoproliferative syndrome in these mice. CD4+ T cells in LATY136F mice are Th2 skewed, producing large amounts of IL-4. In this study, we showed that the LATY136F T cells could also overproduce IL-6 due to activated NF-κB, AKT, and p38 pathways. By crossing LATY136F mice with IL-6–deficient mice, we demonstrated that IL-6 is required for uncontrolled T cell expansion during the early stage of disease development. Reduced CD4+ T cell expansion was not due to a further block in thymocyte development or an increase in the number of regulatory T cells, but was caused by reduction in cell survival. In aged IL-6−/− LATY136F mice, CD4+ T cells began to hyperproliferate and induced splenomegaly; however, isotype switching and autoantibody production were diminished. Our data indicated that the LAT–PLCγ1 interaction is important for controlling IL-6 production by T cells and demonstrated a critical role of IL-6 in the development of this lymphoproliferative syndrome.
Differential Features of AIRE-Induced and AIRE-Independent Promiscuous Gene Expression in Thymic Epithelial Cells
Authors: St-Pierre Charles , Trofimov Assya , Brochu Sylvie , Lemieux Sébastien , Perreault Claude 
Published: June 2015

Abstract
Establishment of self-tolerance in the thymus depends on promiscuous expression of tissue-restricted Ags (TRA) by thymic epithelial cells (TEC). This promiscuous gene expression (pGE) is regulated in part by the autoimmune regulator (AIRE). To evaluate the commonalities and discrepancies between AIRE-dependent and -independent pGE, we analyzed the transcriptome of the three main TEC subsets in wild-type and Aire knockout mice. We found that the impact of AIRE-dependent pGE is not limited to generation of TRA. AIRE decreases, via non–cell autonomous mechanisms, the expression of genes coding for positive regulators of cell proliferation, and it thereby reduces the number of cortical TEC. In mature medullary TEC, AIRE-driven pGE upregulates non-TRA coding genes that enhance cell–cell interactions (e.g., claudins, integrins, and selectins) and are probably of prime relevance to tolerance induction. We also found that AIRE-dependent and -independent TRA present several distinctive features. In particular, relative to AIRE-induced TRA, AIRE-independent TRA are more numerous and show greater splicing complexity. Furthermore, we report that AIRE-dependent versus -independent TRA project nonredundant representations of peripheral tissues in the thymus.
Cutting Edge: Roles for Batf3-Dependent APCs in the Rejection of Minor Histocompatibility Antigen–Mismatched Grafts
Authors: Atif Shaikh M. , Nelsen Michelle K. , Gibbings Sophie L. , Desch A. Nicole , Kedl Ross M. , Gill Ronald G. , Marrack Philippa , Murphy Kenneth M. , Grazia Todd J. , Henson Peter M. , Jakubzick Claudia V. 
Published: June 2015

Abstract
In transplantation, a major obstacle for graft acceptance in MHC-matched individuals is the mismatch of minor histocompatibility Ags. Minor histocompatibility Ags are peptides derived from polymorphic proteins that can be presented by APCs on MHC molecules. The APC subtype uniquely responsible for the rejection of minor Ag–mismatched grafts has not yet been identified. In this study, we examined graft rejection in three mouse models: 1) mismatch of male-specific minor Ags, 2) mismatch of minor Ags distinct from male-specific minor Ags, and 3) skin transplant. This study demonstrates that in the absence of pathogen-associated molecular patterns, Batf3-dependent dendritic cells elicit the rejection of cells and grafts expressing mismatched minor Ags. The implication of our findings in clinical transplantation may be significant, as minor Ag reactivity has been implicated in the pathogenesis of multiple allograft tissues.