bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapeutic potential of ixmyelocel-T, an expanded autologous multicellular therapy for treatment of ischemic cardiovascular diseases
Authors: Ledford Kelly J , Murphy Nikki , Zeigler Frank , Bartel Ronnda L , Tubo Ross 
Published: March 2015

Abstract
Introduction
Bone marrow derived cellular therapies are an emerging approach to promoting therapeutic angiogenesis in ischemic cardiovascular disease. However, the percentage of regenerative cells in bone marrow mononuclear cells (BMMNCs) is small, and large amounts of BMMNCs are required. Ixmyelocel-T, an expanded autologous multicellular therapy, is manufactured from a small sample of bone marrow aspirate. Ixmyelocel-T contains expanded populations of mesenchymal stromal cells (MSCs) and M2-like macrophages, as well as many of the CD45+ cells found in the bone marrow. It is hypothesized that this expanded multi-cellular therapy would induce angiogenesis and endothelial repair.

Methods
A rat model of hind limb ischemia was used to determine the effects of ixmyelocel-T on blood flow recovery. To further determine the effects on endothelial cells, ixmyelocel-T was co-cultured with human umbilical vein endothelial cells (HUVEC) in non-contacting Transwell® inserts.

Results
Co-culture of HUVECs with ixmyelocel-T resulted secretion of a variety of pro-angiogenic factors. HUVECs stimulated by ixmyelocel-T exhibited enhanced migration, proliferation, and branch formation. Ixmyelocel-T co-culture also resulted in increased endothelial nitric oxide synthase (eNOS) expression and nitric oxide (NO) production. In tumor necrosis factor alpha (TNFα)-stimulated HUVECs, ixmyelocel-T co-culture decreased apoptosis and reactive oxygen species generation, increased super oxide dismutase activity, and decreased nuclear factor kappa B (NFκB) activation. Treatment with ixmyelocel-T in a rat model of hind limb ischemia resulted in significantly increased blood flow perfusion and capillary density, gene expression and plasma levels of the anti-inflammatory cytokine interleukin (IL)-10, plasma nitrates, plasma platelet-derived growth factor (PDGF)-BB, vascular endothelial growth factor (VEGF) expression, and significantly decreased plasma thiobarbituric acid reactive substances (TBARS).

Conclusions
This work demonstrates that ixmyelocel-T interacts with endothelial cells in a paracrine manner, resulting in angiogenesis and endothelial protection. This data suggests that ixmyelocel-T could be useful for promoting of angiogenesis and tissue repair in ischemic cardiovascular diseases. In conclusion, ixmyelocel-T therapy may provide a new aspect of therapeutic angiogenesis in this patient population where expanded populations of regenerative cells might be required.
SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model
Authors: Tsartsalis Stergios , Dumas Noé , Tournier Benjamin B , Pham Tien , Moulin-Sallanon Marcelle , Grégoire Marie-Claude , Charnay Yves , Millet Philippe 
Published: March 2015

Abstract

Background
Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.

Methods
GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [123I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.

Results
Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.

Conclusions
The present study demonstrates the feasibility of [123I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.
Power minimization of cooperative beamforming networks with spectrum sharing
Authors: Ling Cen , Yin Xuefeng , Boqué Silvia Ruiz , García-Lozano Mario 
Published: March 2015

Abstract
Cooperative communication with spectrum sharing has been proved to be an efficient way to reduce energy consumption. In this work, an optimal power allocation algorithm is proposed for mobile cooperative beamforming networks, where several secondary users (SUs) collaboratively act as relay nodes to assist the transmission of a primary user (PU) in reward of transmitting their own information. The optimal power allocation scheme is obtained by solving a convex optimization problem aiming at minimizing network energy consumption with guaranteed quality of service (QoS) for both PU and SUs. Simulation results show that with the consideration of overheads, circuit power, and energy costs for message sharing, a cooperative beamforming network augmented with our proposed power allocation algorithm performs better in scenarios of more SUs in terms of higher energy efficiency and less power consumption per unit throughput. Additionally, the proposed scheme outperforms the conventional best-relay selection approach in the aspects of power consumption per unit throughput, energy efficiency, and network power consumption. Furthermore, simulations also demonstrate that the derived cooperative beamforming algorithm exhibits superior performance in energy saving than conventional methods in high-speed scenarios.
Paradoxical Dissociation Between Hepatic Fat Content and De Novo Lipogenesis Due to PNPLA3 Sequence Variant
Authors: Mancina Rosellina M. , Matikainen Niina , Maglio Cristina , Söderlund Sanni , Lundbom Nina , Hakkarainen Antti , Rametta Raffaela , Mozzi Enrico , Fargion Silvia , Valenti Luca , Romeo Stefano , Taskinen Marja-Riitta , Borén Jan 
Published: March 2015

Abstract

Context:
Nonalcoholic fatty liver disease (NAFLD) is an emerging epidemic disease characterized by increased hepatic fat, due to an imbalance between synthesis and removal of hepatic lipids. In particular, increased hepatic de novo lipogenesis (DNL) is a key feature associated with NAFLD. The genetic variations I148M in PNPLA3 and E167K in TM6SF2 confer susceptibility to NAFLD.

Objective:
Here we aimed to investigate the contribution of DNL to liver fat accumulation in the PNPLA3 I148M or TM6SF2 E167K genetic determinants of NAFLD.

Patients and Methods:
The PNPLA3 I148M and TM6SF2 E167K were genotyped in two well-characterized cohorts of Europeans. In the first cohort (Helsinki cohort; n = 88), we directly quantified hepatic DNL using deuterated water. In the second cohort (Milan cohort; n = 63), we quantified the hepatic expression of SREBP1c that we have found previously associated with increased fat content. Liver fat was measured by magnetic resonance proton spectroscopy in the Helsinki cohort, and by histological assessment of liver biopsies in the Milan cohort.

Results:
PNPLA3 148M was associated with lower DNL and expression of the lipogenic transcription factor SREBP1c despite substantial increased hepatic fat content.

Conclusions:
Our data show a paradoxical dissociation between hepatic DNL and hepatic fat content due to the PNPLA3 148M allele indicating that increased DNL is not a key feature in all individuals with hepatic steatosis, and reinforces the contribution of decreased mobilization of hepatic triglycerides for hepatic lipid accumulation in subject with the PNPLA3 148M allele.
Selective Coregulator Function and Restriction of Steroid Receptor Chromatin Occupancy by Hic-5
Authors: Chodankar Rajas , Wu Dai-Ying , Gerke Daniel S. , Stallcup Michael R. 
Published: March 2015

AbstractSteroid receptors (SRs) bind specific DNA regulatory sequences, thereby activating and repressing gene expression. We previously showed that transcriptional coregulator Hic-5 facilitates glucocorticoid regulation of some genes but blocks glucocorticoid regulation of others. Here, in a genome-wide analysis, Hic-5 depletion dramatically increased the global number of sites occupied by glucocorticoid receptor (GR) α (the major GR isoform), and many binding sites blocked by Hic-5 were associated with genes for which Hic-5 also blocked glucocorticoid-regulated expression. Hic-5 had similar effects on GRγ (a splice variant of GRα) and estrogen receptor α (ERα), facilitating hormonal regulation of some genes and blocking hormonal regulation of others. As with GRα, Hic-5 blocking of hormonal gene regulation mediated by GRγ and ERα was associated with blocking of GRγ and ERα occupancy at nearby sites. Hic-5 supported hormonal regulation of many more genes for GRα than for GRγ or ERα and thus exhibited selective coregulator functions for different SRs. In contrast, the number of Hic-5–blocked genes was similar for all 3 SRs. In addition to classic coregulator activity, Hic-5 influences the genomic occupancy of multiple SRs and thereby blocks some aspects of hormonal regulation. Thus, Hic-5, because of its tissue-specific expression, could contribute to tissue-specific genomic occupancy and gene regulation by SRs.
Poly(1,4-phenylene sulfide) (PPS) Synthesis via Oxidative Polymerization of Diphenyl Disulfide: Mechanistic Insight into the Selective Formation of 1,4-Thiophenylene Chain
Authors: Aida Fuyuki , Takatori Yohei , Kiyokawa Daichi , Nagamatsu Kentaro , Nishide Hiroyuki , Oyaizu Kenichi 
Published: March 2015

Abstract
Oxidative polymerization of diphenyl disulfide under molten conditions at moderately elevated temperatures gave poly(1,4-phenylene sulfide) (PPS), using a vanadyl complex–strong acid catalyst with oxygen as the ultimate oxidant. Origin of the selectivity of the sulfonium electrophile for the formation of the 1,4-thiophenylene chain, characterized by the presence of a disulfide bond at the chain end, was determined by the behavior of polymerization and structural analysis of the product together with a computational calculation, which revealed a unique chain growth-type polycondensation process.
Creation of Kinetically Stabilized Porphyrin Microfilms through Synchronized Hydrogen-bonding Interactions in Microflow
Authors: Numata Munenori , Nishino Yuri , Sanada Yusuke , Sakurai Kazuo 
Published: March 2015

Abstract
Fine hydrogen-bonded networks over the micrometer scale were spontaneously constructed in microflow through the synchronized protonation of porphyrin having four carboxylic acid groups. The obtained porphyrin-based microfilms were kinetically stabilized by the hydrogen-bonded networks, and therefore were easily decomposed through synchronized deprotonation in the microflow.
A new quality of life consultation template for patients with venous leg ulceration
Authors: Green J. , Jester R. , McKinley R. , Pooler A. , Mason S. , Redsell S. 
Published: March 2015

Objective: Chronic venous leg ulcers (CVLUs) are common and recurrent, however, care for patients predominantly has a focus which overlooks the impact of the condition on quality of life. The aim of this study was to develop a simple, evidence-based consultation template, with patients and practitioners, which focuses consultations on quality of life themes. Method: A nominal group was undertaken to develop a new consultation template for patients with CVLUs based on the findings of earlier qualitative study phases. Results: A user-friendly two-sided A4 template was designed to focus nurse-patient consultations on the quality of life challenges posed by CVLUs. Conclusion: CVLUs impact negatively on the quality of life of the patient but this receives inadequate attention during current consultations. This new template will help to ensure that key concerns are effectively raised, explored and addressed during each consultation. Declaration of interest: The NHS West Midlands Strategic Health Authority funded this study. The authors have no conflicts of interest to declare.
Educação Especial na Perspectiva Inclusiva: Implicações para o Trabalho Docente Articulado no Ensino Médio e Tecnológico
Authors: Costas Fabiane Adela Tonetto , Honnef Claucia 
Published: March 2015

O trabalho docente articulado é proposto como uma adaptação ao ensino colaborativo e prevê o trabalho conjunto entre professores da classe comum e professores de Educação Especial no planejamento, no desenvolvimento e na avaliação da aula. O objetivo deste estudo foi analisar como os professores do Ensino Médio e Tecnológico de um Instituto Federal de Educação no Rio Grande do Sul, Brasil, percebem o trabalho docente articulado, quais suas limitações e possibilidades, diante da inclusão de alunos com deficiência e/ou necessidades educacionais especiais (NEE), intensificada a partir da Política Nacional da Educação Especial (Brasil, 2008). A investigação foi qualitativa, com uso de diário de campo e um questionário respondido por sete professores de um aluno com deficiência mental incluído no ensino médio e tecnológico. Para a interpretação dos dados, utilizou-se a Análise de Conteúdo. Os resultados evidenciaram que o trabalho docente articulado é importante. Porém, poucos professores participantes da pesquisa citaram dificuldades para sua realização, evidenciadas pelo baixo número de professores de Educação Especial e de classe comum e o pouco tempo para realização desse trabalho. Concluiu-se que o trabalho docente articulado tem obstáculos a superar, no entanto, acredita-se no seu potencial para mobilizar a aprendizagem, principalmente, dos alunos com deficiência/NEE, frente à Educação Especial na perspectiva da educação inclusiva, a qual, no contexto educacional atual, massificou a ideia de inclusão educacional escolar, sem haver, ainda, investimentos suficientes que garantam condições para que ela possa ser estendida a todos os sujeitos.
The relationship of fibroblast growth factors 21 and 23 and α-Klotho with platelet activity measured by platelet volume indices
Authors: Takeda Yoshihiro , Fujita Shu-ichi , Ikemoto Toshiyuki , Okada Yoshikatsu , Sohmiya Koichi , Hoshiga Masaaki , Ishizaka Nobukazu 
Published: March 2015

AbstractSubjects with high fibroblast growth factor 21(FGF21) and 23 (FGF23), endocrine hormones that regulate insulin sensitivity and phosphate metabolism, respectively, are reported to have a higher risk for adverse cardiovascular outcome. Therefore, the relationship of FGF21, FGF23, and α-Klotho (co-receptor for FGF23 signaling) with mean platelet volume (MPV) and platelet distribution width (PDW), two platelet volume indices that reflect platelet activity, was investigated.Data from 156 patients admitted to the cardiology department were analyzed. MPV and PDW were measured by an automatic blood counter, and serum FGF21, FGF23, and α-Klotho concentrations were measured by an enzyme-linked immunoassay.Log(FGF21) was significantly correlated with serum triglycerides but did not differ according to the use of non-use of antidiabetic or lipid-lowering drugs. MPV and PDW were significantly correlated (R=0.475, p<0.001). MPV was significantly correlated with log(FGF21) (R=–0.167, p<0.05) and log(FGF23) (R=0.351, p<0.001) but not with log(α-Klotho). Linear regression analysis showed a negative and positive association of log(FGF21) and log(FGF23), respectively, with MPV that was independent of possible confounders including sex, age, renal function, and antithrombotic drug use. In addition, log(FGF23) was found to have a significant independent positive association with PDW.Among cardiac patients, FGF21 had a negative association with MPV, whereas FGF23 had a positive association. Future studies of serum FGF23/FGF21 concentrations and the incidence of thromboembolic disorders are warranted.
Evaluation of the Effect of Dynamic Sodium on the Low Cycle Fatigue Properties of 316L(N) Stainless Steel Base and Weld Joints
Authors: Ganesan V. , Kannan R. , Mariappan K. , Sukumaran G. , Sandhya R. , Rao K. Bhanu Sankara 
Published: March 2015

Abstract
Low cycle fatigue (LCF) tests on 316L(N) austenitic stainless steel base and weld joints were at 823 K and 873 K at a constant strain rate of 3 × 10
-3
s
-1
with strain ranges varying from {±}0.4% to {±}1.0% in a servo-hydraulic fatigue test system under flowing sodium environment. The cyclic stress response exhibited a similar trend as that in air comprising of an initial rapid hardening, followed by a slight softening stage before saturation. The fatigue lives are significantly improved in sodium environment when compared to identical testing conditions in air environment. The lack of oxidation in sodium environment is attributed to the delayed crack initiation, reduced crack propagation rate and consequent increase in fatigue life. Comparison of the data evaluated in sodium with RCC-MR design code, derived on the basis of data obtained from air shows that the design based on air tests is conservative.
Photo and Thermal Behavior of New Reinforced Polyamide-nanocomposite Montmorillonite on 2,3-Pyrazin Dicarboxylic Acid
Authors: Faghihi Khalil , Samiei Mojtaba , Hajibeygi Mohsen 
Published: March 2015

AbstractTwo new samples of reinforce polyamidemontmorillonite nanocomposites were synthesized by a convenient solution intercalation technique. Polyamide (PA)
Thermodynamic Properties of Lead Oxide in a Mixture of Stainless Steelmaking and Nonferrous Smelting Slags
Authors: Maruoka Nobuhiro , Ueda Shigeru , Shibata Hiroyuki , Yamaguchi Katsunori , Kitamura Shin-ya 
Published: March 2015

AbstractIn our previous paper, a slag modification process involving the mixing of stainless steelmaking and nonferrous smelting slags was proposed for preventing the disintegration of the stainless steelmaking slag. In order to use this method, the behavior of heavy metals especially PbO contained in the nonferrous slag has to be assessed. In the present study, the activity coefficient of PbO in CaO-SiO
Preparation and Structural Characterizations of Titania Slag
Authors: Chen Guo , Chen Jin , Srinivasakannan C. , Peng Jinhui 
Published: March 2015

AbstractThe thermal stability, crystal structures, microstructure, surface chemical functional groups and molecular structures of titania slag were characterized using TG/DSC, XRD, SEM, FT-IR and Raman spectroscopy techniques, respectively. Occurrence of two phase transformation of titania slag was identified at temperatures of 851 K and 1123 K. The weight gain of samples was observed to increase with increase in the temperature. The SEM results indicated the surface morphology of the primary particles to be tighter and smoother. Raman spectrum indicated existence of vibration band of anatase TiO
Analysis of the Dependence of Material Properties on Temperature – Steel 1.4122
Authors: Brnic Josip , Turkalj Goran , Vukelic Goran , Brcic Marino 
Published: March 2015

AbstractThis article presents an analysis of experimental data with reference to material properties, short-time creep and relaxation phenomena of 1.4122 steel (AISI 420RM) at high temperatures. The behaviour of the material subjected to uniaxial stress is indicated by engineering stress – strain diagrams. The data referring to tensile strength, 0.2 percent offset yield strength, the modulus of elasticity and total deformation, are shown through tables and curves presenting the dependence of these quantities on temperature. Creep and relaxation curves regarding certain level of stresses at selected temperatures are also shown. In addition, modelling of both creep and relaxation phenomena is presented.
Contact Strength and Cracking of Laminar Ceramics
Authors: Hegedűsov’a Lucia , Ceniga Ladislav 
Published: March 2015

AbstractThe paper deals with the determination of strength of laminar ceramics by mechanical tests in bending and contact modes. The bending and contact modes are simulated by the four-point bending test, and by single-cycle contact test using rollers or spheres, respectively. In general, the determination of strength of ceramic materials results from statistical methods which are usually represented by the Weibull analysis. The Weibull analysis comprises the determination of the characteristic strength
Reactive Interdiffusion: A Method for Making Composition Graded Metal-ceramic Composites
Authors: Reddy S. N. S. , Jacob K. T. , Rajitha G. 
Published: March 2015

AbstractPresented is a new method for making composition graded metal-ceramic composites using reactive interdiffusion between a metal and a complex ceramic. Composition variation in both metal and ceramic phases with distance along the direction of diffusion is achieved. The design criteria for developing such composites are discussed. The system should exhibit extensive solid solubility in both metallic and ceramic phases, a defined gradation in the stabilities of the oxides, and mobility of electrons or holes in the oxide solid solution. The complex ceramic used for making the composite should be polycrystalline with sufficient porosity to accommodate the volume expansion caused by alloy precipitation. An inert atmosphere to prevent oxidation and high processing temperature to facilitate diffusive transport are required. The process is illustrated using the reaction couples Fe-NiTiO
Some Insights into Mechanisms Involved in Continuous Casting
Authors: Mills K. C. , Ramirez-Lopez P. E. , Lee P. D. 
Published: March 2015

AbstractA mathematical model of the continuous casting process for steel has been developed. This coupled model predicts metal flow, slag infiltration, shell solidification and heat flux. The model predicts values for the thicknesses of the solid and liquid slag films and the solidified shell, powder consumption and heat fluxes, all of which are in excellent agreement with plant data. The model has been used to study the mechanisms involved in (i) slag entrapment, (ii) the effect of mould oscillation on powder consumption, heat flux and shell solidification, (iii) oscillation mark (OM) formation and (iv) high and low frequency fluctuations.
New Poly(amide-imide)/Nanocomposites Reinforced Silicate Nanoparticles Based on N-pyromellitimido-L-phenyl Alanine Containing Ether Moieties
Authors: Faghihi Khalil , Shabanian Meisam , Dadfar Ehsan 
Published: March 2015

AbstractA series of Poly(amide-imide)/montmorillonite nanocomposites containing
Wear Characteristics of Sintered Cermets
Authors: Bidulský Róbert , Bidulská Jana , Arenas Freddy , Grande Marco Actis 
Published: March 2015

AbstractThe present paper deals with the tribological behaviour of the boride and carbide hardmetals evaluated by performing comparative dry sliding pin-on-disc experiments using normal contact loads. Analyses of the wear performance results, microstructural evaluation and processing conditions effect indicate that microstructure inhomogenities play an important role in abrasive wear behaviour of cermets. In term of grain size and chemical composition, the addition of VC also play an important role in increasing the wear resistance.
Effects of Nano Powder Synthesis Methods, Shaping and Sintering Conditions on Microstructure and Oxygen Permeation of La0.6Sr0.4Co0.2Fe0.8O3-Δ (LSCF) Perovskite-type Membranes
Authors: Asadi Amir Atabak , Behrouzifar Amir , Mohammadi Toraj , Pak Afshin 
Published: March 2015

AbstractLa
Kinetics and Mechanism of Phosphorus Removal from Silicon in Vacuum Induction Refining
Authors: Safarian Jafar , Tangstad Merete 
Published: March 2015

AbstractVacuum induction refining is a process that can be applied to remove phosphorus from molten silicon for the production of solar grade silicon. Pure silicon was doped by phosphorus to make molten silicon containing around 17 ppmw phosphorus. The kinetics of phosphorus removal from this silicon was studied at 0.5 Pa through the application of vacuum induction refining. It was observed that vacuum removal of phosphorus occurs through a first-order reaction. The rate constants of phosphorus evaporation were determined as 2.28