bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Acupuncture Intervention on Chronic Musculoskeletal Pain in HDKF Patients: Study Protocol for A Randomized Controlled Clinical Trial
Authors: Yan Cui-Na , Liu Hui-Rong , Kong Qi , Gan Jiayi , Liu Kun , Yao Wei-Guo , Yao Xing-Mei 
Published: July 2024

Abstract

Background:Chronic musculoskeletal pain is prevalent in individuals with hemodialysis-dependent renal failure (HDKF). The current opioid crisis highlights the urgent need for effective non-pharmacological pain management. Acupuncture, identified as a non-pharmacological intervention in clinical settings, holds promise for alleviating chronic musculoskeletal pain in HDKF patients, but well-designed studies assessing its specific effects in this population are lacking. This study aims to comprehensively evaluate the efficacy and safety of acupuncture as a treatment modality for HDKF patients with chronic musculoskeletal pain.
Methods: This trial, conducted at a single center, follows a parallel, three-arm design with randomization and sham control. 90 HDKF patients with chronic musculoskeletal pain will be assigned randomly to three groups (acupuncture, sham acupuncture, and waiting-list control) in a 1:1:1 ratio. The acupuncture and sham acupuncture groups will undergo 24 treatment sessions over eight weeks, while the waiting-list control group will receive no acupuncture. The primary outcome measures changes in the Numeric Rating Scale (NRS) score from baseline at the end of treatment, with secondary outcomes including the ESAS-R, POS-renal, and HADS scales. Adverse events will be recorded throughout the study, and all randomized patients will be included in the intention-to-treat analysis.
Discussion: The outcomes of this research aim to advance the systematic management of chronic musculoskeletal pain in HDKF patients through non-pharmacological interventions. Moreover, it will contribute insights into personalized treatment strategies, integrating individual expectations and genetic biomarkers to provide "precision" acupuncture for HDKF patients experiencing chronic musculoskeletal pain.
Trial registration: The trial registration number is ChiCTR2400080488. This trial was prospectively registered on 30 January 2024 in the Chinese Clinical Trial Registry.
Comparative Evaluation of Osteogenic Differentiation Capability and Antimicrobial Effect of Cissus Quadrangularis and Laccifer Lacca Extract Coated Dental Implants against Non-Coated Dental Implants- An In Vitro Study
Authors: Deshmukh Shruti , Pisulkar Sweta , Wanjari Anita , Bansod Akansha , Beri Arushi , Jain Ritul 
Published: July 2024

Abstract

Background: Over the past few decades, implantology has achieved numerous advancements. The fundamentals of integrated osteo-implantology have changed significantly, which is altering the therapeutic approaches that the field uses, achieving outstanding outcomes in oral rehabilitation and resolving functional, psychological, and aesthetic issues in cases of partial and complete edentulism. Alternative materials have been studied to enhance implants and bone interference, including bone-conductive and bone-inducing materials. By enhancing mineralization, bone matrix synthesis, and bone cell adhesion, osseointegration can be enhanced through nanoscale alteration of implant surface coatings. The goal of the implant surface treatments is to create a surface that is biologically active, enhancing the osseointegration process between the implant and bone tissue. For accelerating bone fractures, two well-known ayurvedic formulations are frequently utilized Cissus quadrangularis and Laccifer lacca. Additionally, these formulations include analgesic and anti-inflammatory qualities that lessen the severity of disorders associated to bones and joints. The study focuses on assessing a modification of surface coated implants with herbal extract of Cissus quadrangularis and Laccifer lacca for osteogenic potential differentiation and antimicrobial properties.
Methods:
1. Titanium discs of diameter 10mm and thickness 2mm will be used.
2. Hydroalcoholic extract of Cissus quadrangularis and aqueous extract of Laccifer lacca will be obtained. Prepared extract will be coated on the titanium discs
3. Four groups will be prepared

Group A: Discs coated with Cissus quadrangularis
Group B: Disc coated with Laccifer lacca
Group C: Disc coated with combination of C.
quadrangularis and L. lacca in 1:1 ratio w/w%.
Group D: Non coated discs

4. Each group will be further divided to perform two tests i.e. Osteoblastic Proliferation test and antimicrobial test.

Discussion: Studies have been carried out with
Cissus quadrangularis and Laccifer lacca that studies suggest that
they promote bone healing. Srinidhi et al in year 2020 carried
out a study with C quadrangularis the study included
spectrophotometric analysis to measure the activities of alkaline
phosphatase and lactate dehydrogenase, as well as to ascertain
their kinetics. Based on the findings, they came to the conclusion
that extracts from C. quadrangularis and Lakshadi guggulu exhibit
strong osteogenic activity and can be effectively utilized as
surface modification materials in titanium implants and dental bone
mending. But there is a lack of research that combines the
properties of both C. quadrangularis and Laccifer lacca as a
surface coating material and hence the study was needed to assess
the same.
Clinical Trial registration Number (CTRI):
REF/2024/05/084317
Effectiveness of the Resistance Exercises with Motor Skills Training and Pain Reprocessing on Pain, Performance, and Psychological Factors in Flight Attendants with Non-Specific Low Back Pain: Study Protocol for a Randomised Controlled Trial
Authors: Zolfaghari Negin , Letafatkar Amir , Rossettini Giacomo 
Published: July 2024

Abstract

Background
Low back pain (LBP) is one of the prevalent chronic pains in flight attendants. Exercise is recommended; however, the effects of resistance exercises with motor skills training and pain reprocessing in flight attendants with LBP are not currently known. This study compares the effect of resistance exercises with motor skills training and pain reprocessing on the pain, performance, and psychological factors of flight attendants with LBP.
Methods
This study is a randomized controlled trial in which 60 flight attendants with LBP will be enrolled. The patients will be randomly allocated to receive (1) resistance exercises plus motor skill exercises, (2) resistance exercises plus pain reprocessing, (3) resistance exercises plus motor skill exercises and pain reprocessing and (4) resistance training. Participants will be assessed pre- and post-intervention and 3 months after interventions. The primary outcome will be pain intensity. The secondary outcomes will be disability, quality-of-life, fear of movement, pain catastrophizing, pain self-efficacy, depression, anxiety, stress, performance, single-limb stance, sitting on Bobath ball and muscular endurance at post-intervention and 3 months.
Discussion
This study will be the first to compare resistance training with motor skills training and pain reprocessing on pain, performance and psychological factors of flight attendants with LBP. As this research is being conducted in one of the low-income countries, the demographic characteristics and results may differ from those of high-income countries. Results may guide clinicians and improve their clinical outcomes when treating flight attendants with LBP.
Trial registration:
The protocol was registered prospectively on Clinical Trials (IRCT20220804055617N1, Registration Date: 17/12/2022, http://www.irct.ir).
Application of artificial intelligence in dental crown prosthesis: A scoping review
Authors: Kong Hyun Jun , Kim Yu Lee 
Published: July 2024

Abstract

Background
This scoping review aims to present the applications and performance of artificial intelligence (AI) in dental crown prostheses and related topics.
Methods
We conducted a literature search of PubMed, Scopus, Web of Science, Google Scholar, and IEEE Xplore databases from January 2010 to January 2024. The included articles addressed the application of AI in various aspects of dental crown treatment, including fabrication, assessment, and prognosis.
Results
The initial electronic literature search yielded 393 records, which were reduced to 315 after eliminating duplicate references. The application of inclusion criteria led to analysis of 12 eligible publications in the qualitative review. The AI-based applications included in this review were related to detection of dental crown finish line, evaluation of AI-based color matching, evaluation of crown preparation, evaluation of dental crown designed by AI, identification of a dental crown in an intraoral photo, and prediction of debonding probability.
Conclusions
AI has the potential to increase efficiency in processes such as fabricating and evaluating dental crowns, with a high level of accuracy reported in most of the analyzed studies. However, a significant number of studies focused on designing crowns using AI-based software, and these studies had a small number of patients and did not always present their algorithms. Standardized protocols for reporting and evaluating AI studies are needed to increase the evidence and effectiveness.
Efficacy of Salviae miltiorrhizae and ligustrazine hydrochloride injection on NIHSS, activity of daily living, hemorheology and blood lipid indexes in patients with acute ischemic stroke: a protocol for systematic review and meta analysis
Authors: zhang weiwei , Li Tai , Sun Qianru , Dai Bo 
Published: July 2024

Abstract

Background: Salviae miltiorrhizae and ligustrazine hydrochloride injection (SMLHI), a kind of Chinese herbal medicine injection, has been considered as a promising supplementary treatment for acute ischemic stroke (AIS). However, its clinical efficacy is still not well investigated. In this study, the randomized controlled trials (RCTs) of SMLHI for the treatment of AIS were systematically reviewed to evaluate its clinical efficacy and safety.
Methods and analysis: All available randomized controlled trials (RCTs) that investigated the efficacy and safety ofSMLHI for patients with AIS were searched from the following electronic databases: Google Scholar, PubMed, Cochrane Library, Medline, Embase, Web of Science (WOS), Chinese Biomedical Literature Database (CBLD), China National Knowledge Infrastructure (CNKI), China Scientific Journal Database (CSJ) and Wanfang Database. Papers in Chinese or English published from their inception to December 2023 will be included without any restrictions.
Study selection and data extraction will be performed independently by 2 investigators. The clinical outcomes including neurological deficit score (NIHSS), activity of daily living (ADL), hemorheology and blood lipid indexes were systematically evaluated. Stata 14.0 and Review Manager 5.3 were used for data synthesis, sensitivity analysis, meta regression, subgroup analysis, and risk of bias assessment.
Discussion: The findings of this analysis will provide a helpful evidence for clinicians to formulate the best postoperative adjuvant treatment strategy for patients with AIS, and also provide scientific clues for researchers in this field.
Trial registration number: INPLASY202450033
Spatial patterns and determinants of severe geomorphological changes due to the extreme flood event in the Ahr valley, western Germany in July 2021
Authors: Weidt Fabian , Bell Rainer , Schrott Lothar , Brenning Alexander , Dietze Michael , Burghardt Lisa , Groeßer Joshua 
Published: July 2024

Abstract

The extreme flood event of July 14/15, 2021 caused massive geomorphological changes along the Ahr river in western Germany. The processes include mass movement and bank erosion, channel displacement and widening and deposition of material at the floodplains, all of which contributed to extreme damage. With the aim of gaining a more comprehensive understanding of the factors controlling these processes, spatial patterns of geomorphological changes on a regional scale are analyzed. A differential terrain model (DoD), calculated from digital terrain models (DTM) collected before and after the event using airborne laser scanning (ALS), serves as the data basis. The course of the river is divided into 120 m wide and 100 m long segments. Analyzing the cumulated volumetric loss per segment, which represents the explained variable proxying spatial variability in flood power, is conducted by using a multiple linear regression model. The independent variables considered in this investigation include peak discharge, valley floor width and river curvature. Both the native data and the residuals of the regression model are used to examine effects of bridge failure and subsequent outburst waves on volumetric loss. The analysis shows that the strongest geomorphological changes are associated with high peak discharge and a small valley floor width. River segments containing destroyed arch bridges show higher volumetric loss values than segments with destroyed slab bridges, intact bridges or no bridge at all. These findings provide slight evidence that there are construction types more appropriate than traditional arch bridges to prevent local augmentation of flood power caused by outburst waves resulting from bridge clogging and failure.
On Your Mark, Get Set, Choose! A Randomized Cross-Over Study Comparing Fixed and Self-Selected Rest Periods in Interval Running Among Professional Female Soccer Players.
Authors: Ben-Ari Asaf , Silverman Yedidya , Obolski Uri , Halperin Israel 
Published: July 2024

Abstract

Background
Studies on rest durations during high-intensity interval training (HIIT) often compare fixed and self-selected (SS) rest allocation approaches. Frequently, the rest duration under SS conditions is unlimited, leading to inconsistent total rest durations compared to fixed rest conditions. To address this limitation, we recently compared fixed and SS rest conditions during cycling HIIT sessions, while keeping the total rest time equivalent. However, our protocol required athletes to divide a long total rest time (12 minutes) across nine intervals, which may have been overly cognitively demanding. In the current study, we simplified the athletes’ rest allocation task by reducing the number of rest periods available.
Methods
Following a familiarization session, 24 professional female soccer players completed two running HIIT sessions on a non-motorized treadmill. Each session consisted of twelve 15-second intervals, divided into three blocks, with the goal of maximizing the distance covered. In both conditions, the between-interval rest duration per block amounted to 270 seconds. In the fixed condition, the rest was uniformly allocated to 90 seconds between each interval, whereas in the SS condition, the athletes chose how to allocate the entirety of the 270 seconds of rest. We compared the following outcomes: distance, heart-rate, perception of fatigue, effort, autonomy, enjoyment, boredom, and athletes’ preferences. Outcomes were compared using aggregated measures via paired univariate tests, and across the intervals via mixed-effects models.
Results
We observed comparable results in most outcomes with the exception of higher autonomy in the SS condition (mean difference = 2.1, 95%CI (0.9, 3.3)) and a negligibly higher heart-rate when comparing the observations across intervals (estimate = 2.5, 95%CI (0.9, 4.2)). Additionally, participants chose to rest for longer durations as the block progressed. Finally, most participants (65%) favored the SS condition.
Conclusion
This study further solidifies that SS and fixed approaches with matched total rest durations result in similar performance, physiological, and psychological responses. This effect persists even when the total rest duration required to be allocated is relatively short. Therefore, coaches and trainees can choose either approach based on their preferences and training goals.
Identification and Evaluation of Metabolic mRNAs and Key miRNAs in Colorectal Cancer Liver Metastasis
Authors: chen Guanxuan , Zhang Meng , Wang Shiwen , Shi Wenna , wang Ruoyu , Zhu Wanqi 
Published: July 2024

Abstract

Background
Colorectal cancer (CRC) represents a major global health challenge due to its high lethality, largely attributable to liver metastasis. Despite the established correlation between metabolic reprogramming of cancer cells and their proliferation, invasion, and metastasis, the specific role of metabolism-associated mRNAs in the liver metastasis of CRC remains unelucidated.
Methods
In our research, we procured and analyzed CRC liver metastasis-associated datasets from the GEO database. Subsequently, we employed Weighted Gene Co-expression Network Analysis (WGCNA) to construct an integrated co-expression network of mRNAs and miRNAs, facilitating the identification of pivotal mRNAs and miRNAs. We screened the featured genes using a machine-learning technique, followed by an evaluation of their diagnostic potential for CRC liver metastasis. Additionally, we conducted a functional enrichment analysis and constructed a network of miRNA-targeted mRNAs. Lastly, leveraging the UCSC Xena database, we assessed the correlation between core mRNAs and the clinical attributes and prognosis of CRC patients. Clinical samples from CRC patients and healthy volunteers were collected for validation using qRT-PCR.
Results
Our study identified twelve key mRNAs and four key miRNAs. Functional enrichment analysis indicated that these key genes were intricately linked with biological processes like lipid transport, homeostasis, and metabolism. By implementing LASSO and SVM algorithms, we pinpointed six core mRNAs from the key mRNAs. Their expression patterns and diagnostic performance were validated across multiple datasets. Particularly, CAV1 demonstrated a robust ability to discern between CRC and CRC liver metastasis samples. Additionally, we discerned two key miRNAs (hsa-miR-1246 and hsa-miR-1290) exhibiting diagnostic performance. Lastly, our findings indicate a significant association between AGT, FABP4, and GPD1L and the prognosis of CRC patients. PCR validation in 40 paired tissue samples showed significant downregulation of CAV1 and upregulation of miRNA-1290 in CRC tissues.
Conclusions
This investigation identified modular genes and miRNAs linked to CRC liver metastasis, along with metabolism-associated differentially expressed mRNAs. These pivotal mRNAs and miRNAs could be instrumental in elucidating the biological mechanisms underpinning CRC liver metastasis and offering potential therapeutic targets.
Palliative Effect of Phenolic Extract Derived from Olive Mill Solid Wastes on Pomegranate Plants Submitted to Water Stress
Authors: Tlili Darine , ABBOUD Samia , OUNI Azhar , DBARA Soumaya 
Published: July 2024

Abstract

Fruit trees especially pomegranate (Punica granatum L.) is considered a sensitive specie that showed physiological disturbances under water stress conditions. In this context, it is imperative to improve strategies to mitigate productivity losses and improve fruit quality. On the other hand, Tunisia is an important producer of olive oil and generates every year huge quantities of olive mill solid waste (OMSW). The present study aims the valorization of the OMSW by producing a biostimulant derived from polyphenols extraction. It was applied to pomegranate to restore the negative effects of water stress and as a sustainable alternative to harmful agrochemicals products. The experiment consists on the application of phenolic extract (+PE) at 300 ppm on pomegranate plants. Three water treatments were applied: control (C: 100% FC), moderate stress (MS: 50% FC) and severe stress (SS: 25% FC). In total six combinations there were C+PE, C, MS+PE, MS, SS+PE, SS. Vegetative growth, biochemical statute of leaves and fluorescence were assessed. Results showed that plants treated with PE presented a better tolerance to water stress which was reflected by a higher antioxidant activity, polyphenols, flavonoids, and sugar contents. Additionally, the application of PE decreased the accumulation of proline and stimulated fluorescence increasing leaf area and chlorophyll pigments. It also had a potential in ameliorating shoot elongation and the relative water content in leaves. In conclusion, PE as promoting biostimulant, has beneficial effects by enhancing the tolerance of pomegranate plants to water stress, providing advantages in practices that ensure sustainable agriculture.
Impact of disruptions in antigen processing and presentation machinery on sarcoma
Authors: Renne Salvatore Lorenzo , Samà Laura , Kumar Sonia , Mintemur Omer , Ruspi Laura , Santori Ilaria , Sicoli Federico , Bertuzzi Alexia Francesca , Laffi Alice , Bonometti Arturo , Colombo Piergiuseppe , D'Amato Vittoria , Bressan Alessandra , Scorsetti Marta , Terracciano Luigi , Navarria Pierina , D'Incalci Maurizio , Quagliuolo Vittorio Lorenzo , Pasqualini Fabio , Grizzi Fabio , Cananzi Ferdinando Carlo Maria 
Published: July 2024

Abstract

Background: The antigen processing machinery (APM) plays a critical role in generating tumor-specific antigens that can be recognized and targeted by the immune system. The status of the APM in sarcomas is not well characterized.
Methods: We investigated 126 patients with 8 types of bone and soft tissue sarcoma operated between 2001-2021. Tissue microarrays mapped 11 specific areas in each case. The presence/absence of APM protein was determined through immunohistochemistry. Bayesian networks were used.
Results: All investigated sarcomas had some defects in APM. The least damaged component was HLA Class I subunit β2-microglobulin and HLA Class II. The proteasome LMP10 subunit was defective in leiomyosarcoma (LMS), myxoid liposarcoma (MLPS), and dedifferentiated liposarcoma (DDLPS), while MHC I transporting unit TAP2 was altered in undifferentiated pleomorphic sarcoma (UPS), gastrointestinal stromal tumor (GIST), and chordoma (CH). Among different neoplastic areas, high-grade areas showed different patterns of expression compared to high lymphocytic infiltrate areas. Heterogeneity at the patient level was also observed. Loss of any APM component was prognostic of distant metastasis (DM) for LMS and DDLPS and of overall survival (OS) for LMS.
Conclusion: Sarcomas exhibit a high degree of defects in APM components, with differences among histotypes and tumoral areas. The most commonly altered APM components were HLA Class I subunit β2-microglobulin, HLA Class I subunit α (HC10), and MHC I transporting unit TAP2. The loss of APM components was prognostic of DM and OS and clinically relevant for LMS and DDLPS. This study explores sarcoma molecular mechanisms, enriching personalized therapeutic approaches.