bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treadmill exercise ameliorates symptoms of methimazole‐induced hypothyroidism through enhancing neurogenesis and suppressing apoptosis in the hippocampus of rat pups
Authors: Shin Mal‐Soon , Ko Il‐Gyu , Kim Sung‐Eun , Kim Bo‐Kyun , Kim Tae‐Soo , Lee Shin‐Ho , Hwang Dong‐Sup , Kim Chang‐Ju , Park Jin‐Kook , Lim Baek‐Vin 
Published: January 2013

AbstractThyroid hormones play a crucial role in new neuron production and maturation during brain development. Physical exercise is known to promote cell survival and functional recovery after brain injuries. In the present study, we investigated the effects of treadmill exercise on short‐term memory, spatial learning ability, neurogenesis, and apoptosis in hypothyroidism rat pups. On the 14th perinatal day, the pregnant rats were divided into two groups: the maternal control group and the maternal methimazole (MMI)‐treated group. For the induction of hypothyroidism in rat pups, MMI was added to the drinking water (0.02%, wt/vol), from the 14th prenatal day to the 49th postnatal day. After delivery, the male rat pups born from the maternal control group were assigned into the control group and the control and exercise group. The rat pups born from the maternal MMI‐treated group were divided into the hypothyroidism‐induction group and the hypothyroidism‐induction and treadmill exercise group. The rat pups in the exercise groups were forced to run on a motorized treadmill for 30 min once a day, starting on the 22nd postnatal day for 4 weeks. Induction of hypothyroidism during the fetal and early postnatal period showed suppression of neurogenesis and enhancement of apoptosis in the hippocampus. Short‐term memory and spatial learning ability were deteriorated in the hypothyroidism rat pups. Treadmill exercise during the postnatal period increased neurogenesis and inhibited apoptosis, and resulted in the improvement of short‐term memory and spatial learning ability in the hypothyroidism rat pups.
Rosuvastatin‐regulated post‐translational phosphoproteome in human umbilical vein endothelial cells
Authors: Huang Bin , Wu Chien‐Hsing , Li Fu‐An , Liang Shih‐Shin , Shieh Ying‐Hua , Wang Ling Danny 
Published: January 2013

AbstractStatins, 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase inhibitors, are widely prescribed as cholesterol‐lowering drugs. Statins have recently been found to have pleiotropic effects that are independent of their lipid‐lowering properties. Phosphorylation of serine, threonine, and tyrosine residues of functional proteins are considered to be important in the endothelial signaling cascade. In this study, protein phosphorylation status in human umbilical vein endothelial cells (ECs) after rosuvastatin treatment was examined. The proteins were collected from rosuvastatin‐treated ECs and then the phosphorylated peptides purified by a Fe3+‐immobilized metal‐affinity chromatography bead system were examined by liquid chromatography–tandem mass spectrometry analysis. Alterations of the phosphorylation status of proteins were noticed after rosuvastatin treatment. There were 277 and 530 phosphorylated proteins identified from the control and rosuvastatin‐treated ECs, respectively. Among those proteins, T78, in addition to S156 of the Ras‐GTPase‐activating protein, was phosphorylated after rosuvastatin treatment. Rosuvastatin reduced the phosphorylation of Y455 in HSP90 protein. Decreased phosphorylation of T211 with a concurrent increase in the T291 phosphorylation of Akt1 was observed under rosuvastatin treatment. Increased S633 phosphorylation was detected in endothelial nitric oxide synthase. Western blot analysis further showed an earlier and greater S633 phosphorylation than that of S1177 in endothelial nitric oxide synthase after rosuvastatin treatment. Changes in the phosphorylation status of these proteins may alter the protein's function and affect endothelial physiology. The current study provides new insights leading to a better understanding of the pleiotropic effects of statins on the vascular system.
Urinary neutrophil gelatinase‐associated lipocalin levels predict cisplatin‐induced acute kidney injury better than albuminuria or urinary cystatin C levels
Authors: Lin Hugo You‐Hsien , Lee Su‐Chu , Lin Sheng‐Fung , Hsiao Hui‐Hua , Liu Yi‐Chang , Yang Wen‐Chi , Hwang Daw‐Yang , Hung Chi‐Chih , Chen Hung‐Chun , Guh Jinn‐Yuh 
Published: January 2013

AbstractCisplatin‐induced acute kidney injury (AKI) is a major concern among clinicians in prescribing cisplatin‐based chemotherapy. This study evaluated and compared the ability of urinary biomarkers, including urinary neutrophil gelatinase‐associated lipocalin (NGAL), cystatin C, and the urinary albumin to creatinine ratio (ACR) to predict cisplatin‐induced AKI. Thirty‐three cancer patients receiving cisplatin‐based chemotherapy were prospectively studied, including 10 (30%) who developed AKI (the study group). Changes of urinary biomarkers were compared at 4 hours, 8 hours, and 12 hours, and 1 day, 2 days, 3 days, and 4 days after cisplatin intravenous infusions (75 mg/m2) versus the baseline. There was a significant increase in urinary NGAL levels from 12 hours to 4 days (p < 0.05) compared to baseline after cisplatin infusion in the AKI group. The magnitude of these changes over time differed significantly by group (p < 0.001). The area under the receiver operating curve describing the relationship between urinary NGAL levels and AKI within 12 hours was 0.865 (95% confidence interval = 0.691–1.000). Urinary NGAL levels independently predicted AKI 12 hours after cisplatin (p = 0.045) after adjustments for age, gender, body mass index, baseline serum creatinine, and urinary total protein. Urinary NGAL levels may be an early biomarker of AKI in patients receiving cisplatin‐based treatment.
The feasibility and correlation of atrial fibrillation vulnerability test to the indices of atrial substrates using atrial burst decremental pacing
Authors: Tang Wei‐Hua , Lee Kun‐Tai , Tsai Wei‐Chung , Sheu Sheng‐Hsiung , Lai Wen‐Ter 
Published: January 2013

AbstractPulmonary vein isolation and atrial substrate modification using catheter ablation have been developed as a standard treatment for atrial fibrillation (AF). However, the standard end‐point for these procedures is still not well established. This study aimed to investigate the characteristics among positive and negative AF vulnerability patients with normal structural heart, in order to define the endpoint of the atrial substrate modification. Fifty supraventricular tachycardia patients with normal heart structure referred for electrophysiological study and catheter ablation were enrolled. After eliminating the underling arrhythmias, the basic cycle length, effective refractory period of the right atrium, and the P wave indices in 12‐lead electrocardiograms were measured. The AF vulnerability test was performed by atrial burst decremental pacing with a pacing cycle length decreasing from 290 ms to 200 ms. The AF vulnerability test was considered as positive when the duration of the induced AF or atrial tachyarrhythmias (ATs) was longer than 10 seconds. The parameters of atrial substrates were compared between patients with positive and negative values of the AF vulnerability test. ATs or AF were induced in 24 (48%) patients. Among these patients, 12 (24%) induced ATs or AF were found to be sustained (duration more than 10 seconds). However, only two of these patients could reproduce the positive result after 10 minutes of the first induced protocol. Comparing the patient baseline characteristics, P wave characteristics and cardiac echo parameters, there were no significant differences between the positive and negative AF vulnerability groups. In conclusion, AF and ATs could be induced in patients with a structurally normal heart. The traditional clinical indices of atrial substrates were not significantly different between the positive and negative AF vulnerability patients. Protocols other than atrial burst decremental pacing should be investigated to evaluate the endpoint of the atrial substrate modification.
Novel usage of intraocular pressure‐lowering drugs as wound‐healing inhibitors after trabeculectomy with cell culture and animal models
Authors: Wu Kwou‐Yeung , Wu Pei‐Cheng , Lai Yu‐Hung , Hong Show‐Jen 
Published: January 2013

AbstractInhibiting the healing of wounds to ensure that the aqueous humor can drain into the scleral space unimpeded and form a filtering bleb plays a crucial role in determining the success rate of glaucoma surgery. The aim of this study was to investigate the novel use, with cell culture and animal models, of some commercial intraocular pressure (IOP)‐lowering drugs in inhibiting the healing of fibroblast wounds. The Tenon's fibroblasts of rabbits were cultured to evaluate 13 IOP‐lowering drugs for cellular proliferation, collagen formation, and migration. These were measured using [3H]thymidine and [3H]proline uptake, and Transwell chambers. A preservative of benzalkonium chloride (BAK) was initially used, with 0.02% as a maximal original concentration. All of the drugs and the BAK were diluted from original commercial concentrations to 1/10, 1/100, and 1/1000. The more inhibitive drugs screened from the cell cultures were then selected for further short‐term application during and after trabeculectomy surgeries had been performed on the rabbits. Expression of the proliferative cell nuclear antigen was immunohistochemically examined 3 and 7 days after surgery. The results revealed that the inhibitive effects of BAK in cellular [3H]thymidine and [3H]proline uptake, and cellular migration were only evident at 0.002% concentrations. Based on the results of the cell cultures, timolol, latanoprost, and unoprostone exhibited a greater inhibitory effect than the other drugs. Moreover, the animal studies showed that latanoprost and unoprostone significantly suppressed the positive expression of proliferative cell nuclear antigen around the operative excision area 7 days after the trabeculectomy surgeries. The results indicate that short‐term use of some IOP‐lowering drugs, such as latanoprost and unoprostone, may inhibit postoperative wound healing after glaucoma surgery.
Randomized trial comparing rabeprazole‐ versus lansoprazole‐based Helicobacter pylori eradication regimens
Authors: Liu Meng‐Kwan , Wu I‐Chen , Lu Chien‐Yu , Kuo Chao‐Hung , Yu Fang‐Jung , Liu Chung‐Jung , Hsu Ping‐I , Hsu Wen‐Hung , Su Yu‐Chung , Chen Angela , Wu Deng‐Chyang , Kuo Fu‐Chen , Chen Jyh‐Jou 
Published: January 2013

AbstractDifferent types of proton pump inhibitor (PPI)‐based triple therapies could result in different Helicobacter pylori eradication rates. This study aimed to compare the efficacy and safety of rabeprazole‐ and lansoprazole‐based triple therapies in primary treatment of H. pylori infection. From September 2005 to July 2008, 426 H. pylori‐infected patients were randomly assigned to receive a 7‐day eradication therapy with either rabeprazole 20 mg bid (RAC group, n = 222) or lansoprazole 30 mg bid (LAC group, n = 228) in combination with amoxicillin 1 g bid and clarithromycin 500 mg bid. The patients received follow‐up esophagogastroduodenoscopy (EGD) and/or 13C‐urea breath test 12–16 weeks later to define H. pylori status. Their personal and medical history, compliance and side effects were obtained by using a standardized questionnaire. Intention‐to‐treat analysis revealed that the eradication rate was 87.84% in the RAC group and 85.96% in the LAC group (p = 0.56). All patients returned for assessment of compliance (100% in the LAC group vs. 99.50% in the RAC group; p = 0.32) and adverse events (7.20% in the RAC group vs. 5.70% in the LAC group, p = 0.51). Univariate analysis suggested that patients with nonsteroid anti‐inflammatory agent (NSAID) use had lower eradication rates than those without (76.71% vs. 88.74%; p = 0.006). Our results showed that efficacy and safety were similar in rabeprazole‐ and lansoprazole‐based primary therapies. The influence of NSAID usage on H. pylori eradication needs to be further investigated.
Adding MUFA to a dietary portfolio of cholesterol-lowering foods reduces apoAI fractional catabolic rate in subjects with dyslipidaemia
Authors: Labonté Marie-Ève , Jenkins David J. A. , Lewis Gary F. , Chiavaroli Laura , Wong Julia M. W. , Kendall Cyril W. C. , Hogue Jean-Charles , Couture Patrick , Lamarche Benoît 
Published: January 2013

The present randomised parallel study assessed the impact of adding MUFA to a dietary portfolio of cholesterol-lowering foods on the intravascular kinetics of apoAI- and apoB-containing lipoproteins in subjects with dyslipidaemia. A sample of sixteen men and postmenopausal women consumed a run-in stabilisation diet for 4 weeks. Subjects were then randomly assigned to an experimental dietary portfolio either high or low in MUFA for another 4 weeks. MUFA substituted 13·0 % of total energy from carbohydrate (CHO) in the high-MUFA dietary portfolio. Lipoprotein kinetics were assessed after the run-in and portfolio diets using a primed, constant infusion of [2H3]leucine and multicompartmental modelling. The high-MUFA dietary portfolio resulted in higher apoAI pool size (PS) compared with the low-MUFA dietary portfolio (15·9 % between-diet difference,P= 0·03). This difference appeared to be mainly attributable to a reduction in apoAI fractional catabolic rate (FCR) after the high-MUFA diet ( − 5·6 %,P= 0·02v. pre-diet values), with no significant change in production rate. The high-MUFA dietary portfolio tended to reduce LDL apoB100 PS compared with the low-MUFA dietary portfolio ( − 28·5 % between-diet difference,P= 0·09), predominantly through an increase in LDL apoB100 FCR (23·2 % between-diet difference,P= 0·04). These data suggest that adding MUFA to a dietary portfolio of cholesterol-lowering foods provides the added advantage of raising HDL primarily through a reduction in HDL clearance rate. Replacing CHO with MUFA in a dietary portfolio may also lead to reductions in LDL apoB100 concentrations primarily by increasing LDL clearance rate, thus potentiating further the well-known cholesterol-lowering effect of this diet.
Perceived thickness and creaminess modulates the short-term satiating effects of high-protein drinks
Authors: Bertenshaw Emma J. , Lluch Anne , Yeomans Martin R. 
Published: January 2013

Previous research suggests that increasing beverage protein content enhances subsequent satiety, but whether this effect is entirely attributable to post-ingestive effects of protein or is partly caused by the distinct sensory characteristics imparted by the presence of protein remains unclear. To try and discriminate nutritive from sensory effects of added protein, we contrasted effects of three higher-energy (about 1·2 MJ) and one lower-energy (LE: 0·35 MJ) drink preloads on subsequent appetite and lunch intake. Two higher-energy drinks had 44 % of energy from protein, one with the sensory characteristics of a juice drink (HP − , low-sensory protein) and the second a thicker and creamier (HP+, high-sensory protein) drink. The high-carbohydrate preload (HC+, high-sensory carbohydrate) was matched for thickness and creaminess to the HP+ drink. Participants (healthy male volunteers,n26) consumed significantly less at lunch after the HP+(566 g) and HC+(572 g) than after HP − (623 g) and LE (668 g) drinks, although the compensation for drink energy accounted for only 50 % of extra energy at best. Appetite ratings indicated that participants felt significantly less hungry and more full immediately before lunch in HP+ and HC+ groups compared with LE, with HP − being intermediate. The finding that protein generated stronger satiety in the context of a thicker creamier drink (HP+ but not HP − ) and that an isoenergetic carbohydrate drink (HC+), matched in thickness and creaminess to the HP+ drink, generated the same pattern of satiety as HP+, both suggest an important role for these sensory cues in the development of protein-based satiety.
Impact of climate change on harvest security and biomass yield of two timothy ley harvesting systems in Norway
Authors: PERSSON T. , HÖGLIND M. 
Published: January 2013

SUMMARYPredicted future climate changes in northern Europe include increased air temperature and altered precipitation patterns. There is a lack of knowledge about potential climate change effects on the biomass yield and security of agricultural crops. The present study determined the potential impact of future climate change on the yield and harvest security of timothy (Phleum pratense L.). Harvest security was assessed using data on accumulated precipitation and the length of dry spell period within the 7 days after cutting. Timothy production as a function of weather, soil and management practices was simulated using the LINGRA model for the periods 1961–90, 2046–65 and 2080–99, and the locations Apelsvoll, Ås, Sola, Tromsø and Værnes in Norway and harvest systems with 600 and 800 °C days between cuts. One hundred years of daily weather data were generated with the LARS-WG tool, using future daily weather data sets based on 12 Global Climate Models. Total seasonal biomass yield varied between 690 g dry matter (DM)/m2 for the 800 °C days harvesting regime in the period 1961–90 at Tromsø and 1548 g DM/m2 for the same harvesting regime in the period 2046–65 at Sola. In general, the biomass was higher in the two future periods than in 1961–90 across locations and harvesting regimes, mainly owing to more cuts per season. Accumulated precipitation after cutting varied between 12·2 mm after the first cut for the 600 °C days harvesting regime in the period 1961–90 at Værnes and 42·5 mm after the fourth cut in the 800 °C days harvesting regime in the period 2080–99 at Sola. The longest duration of dry spell 7 days after pre-planned harvest varied between 1·8 days after the fourth cut at Sola in the 600 °C days harvesting regime for the period 2080–99, and 3·9 days after the first cut at Ås in the 800 °C days harvesting regime for the period 2046–65. Potential consequences of these results are discussed.
Exploring the ameliorative potential of probiotic Dahi containingLactobacillus acidophilusandBifidobacterium bifidumon dextran sodium sulphate induced colitis in mice
Authors: Jadhav Sagar R. , Shandilya Umesh Kr. , Kansal Vinod K. 
Published: January 2013

Conventional medical therapies for ulcerative colitis (UC) are still limited due to the adverse side effects like dose-dependent diarrhoea and insufficient potency to keep in remission for long-term periods. So, new alternatives that provide more effective and safe therapies for ulcerative colitis are constantly being sought. In the present study, probiotic LaBb Dahi was selected for investigation of its therapeutic effect on DSS-induced colitis model in mice. LaBb Dahi was prepared by co-culturing Dahi culture of Lactococci along with selected strain ofLactobacillus acidophilusLaVK2 andBifidobacterium bifidumBbVK3 in buffalo milk. Four groups of mice (12 each) were fed for 17 d with buffalo milk (normal control), buffalo milk plus DSS (Colitis control), Dahi plus DSS, and LaBb Dahi plus DSS, respectively, with basal diet. The disease activity scores, weight loss, organ weight, colon length, myeloperoxidase (MPO) and β-glucoronidase activity was assessed, and the histopathological picture of the colon of mice was studied. All colitis control mice evidenced significant increase in MPO, β-glucoronidase activity and showed high disease activity scores along with histological damage to colonic tissue. Feeding with LaBb Dahi offered significant reduction in MPO activity, β-glucoronidase activity and improved disease activity scores. We found significant decline in length of colon, organ weight and body weight in colitis induced controls which were improved significantly by feeding LaBb Dahi. The present study suggests that LaBb Dahi can be used as a potential nutraceutical intervention to combat UC related changes and may offer effective adjunctive treatment for management of UC.
Socio-economic status over the lifecourse and internet use in older adulthood
Authors: SILVER MICHELLE PANNOR 
Published: January 2013

ABSTRACTThis study explored associations between socio-economic status (SES) at different phases in the lifecourse and regular internet use among older adults. A sample (N = 11,035) from the 2010 wave of the United States Health and Retirement Study was used. Odds ratios were estimated to explore the relationship between regular internet use in older adulthood and measures of SES in childhood and in adulthood, and cumulative SES. Findings provided support for the lifecourse perspective, suggesting that variations observed among older adults are reflective of cumulative experiences. Three main themes emerged: higher SES in childhood increased the odds of being an internet user in older adulthood; SES advantages tended to accumulate, so that having at least one period of high SES in the lifecourse increased the odds of being an internet user in older adulthood; age did not appear to modify the positive relationship between cumulative SES and internet use.
Effects of Moderate to Severe Traumatic Brain Injury on Anticipating Consequences of Actions in Adolescents: A Preliminary Study
Authors: Cook Lori G. , Hanten Gerri , Orsten Kimberley D. , Chapman Sandra B. , Li Xiaoqi , Wilde Elisabeth A. , Schnelle Kathleen P. , Levin Harvey S. 
Published: January 2013

AbstractFor this pilot study, we compared performance of 15 adolescents with moderate–severe traumatic brain injury (TBI) to that of 13 typically developing (TD) adolescents in predicting social actions and consequences for avatars in a virtual microworld environment faced with dilemmas involving legal or moral infractions. Performance was analyzed in relation to cortical thickness in brain regions implicated in social cognition. Groups did not differ in number of actions predicted nor in reasons cited for predictions when presented only the conflict situation. After viewing the entire scenario, including the choice made by the avatar, TD and TBI adolescents provided similar numbers of short-term consequences. However, TD adolescents provided significantly more long-term consequences (p = .010). Additionally, for the Overall qualitative score, TD adolescents’ responses were more likely to reflect the long-term impact of the decision made (p = .053). Groups differed in relation of the Overall measure to thickness of right medial prefrontal cortex/frontal pole and precuneus, with stronger relations for the TD group (p < .01). For long-term consequences, the relations to the posterior cingulate, superior medial frontal, and precentral regions, and to a lesser extent, the middle temporal region, were stronger for the TBI group (p < .01). (JINS, 2013, 19, 1–10.)