bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leptin modulates dose‐dependently the metabolic and cytolytic activities of NK‐92 cells
Authors: Lamas Bruno , Goncalves‐Mendes Nicolas , Nachat‐Kappes Rachida , Rossary Adrien , Caldefie‐Chezet Florence , Vasson Marie‐Paule , Farges Marie‐Chantal 
Published: November 2012

AbstractLeptin, a hormone‐cytokine produced primarily in the adipose tissue, has pleiotropic effects on many biological systems and in several cell types, including immune cells. Hyperleptinemia is associated with immune dysfunction and carcinogenesis. Natural killer (NK) cells are critical mediators of anti‐tumor immunity, and leptin receptor deficiency in mice leads to impaired NK function. It was thus decided to explore the in vitro effects of leptin on human NK cell function. NK‐92 cells were cultured during 48 h with different leptin concentrations [absence, 10 (physiological), 100 (obesity), or 200 ng/ml (pharmacology)]. Their metabolic activity was assessed using the resazurin test. NK‐92 cell cytotoxicity and intracellular IFN‐γ production were analyzed by flow cytometry. NK‐92 cell mRNA and protein expression levels of cytotoxic effectors were determined by RT‐qPCR and Western blot. In our conditions, leptin exerted a dose‐dependent stimulatory effect on NK‐92 cell metabolic activity. In addition, high leptin concentrations enhanced NK‐92 cell cytotoxicity against K562‐EGFP and MDA‐MB‐231‐EGFP target cells and inversely reduced cytotoxicity against the MCF‐7‐EGFP target. At 100 ng/ml, leptin up‐regulated both NK cell granzyme B and TRAIL protein expressions and concomitantly down‐regulated perforin expression without affecting Fas‐L expression. In response to PMA/ionomycin stimulation, the proportion of IFN‐γ expressing NK‐92 cells increased with 100 and 200 ng/ml of leptin. In conclusion, leptin concentration, at obesity level, variably increased NK‐92 cell metabolic activity and modulated NK cell cytotoxicity according to the target cells. The underlying mechanisms are partly due to an up‐regulation of TRAIL and IFN‐γ expression and a down‐regulation of perforin. J. Cell. Physiol. 228: 1202–1209, 2013. © 2012 Wiley Periodicals, Inc.
Phosphorylation of Cdc6 at serine 74, but not at serine 106, drives translocation of Cdc6 to the cytoplasm
Authors: Yim Hyungshin , Park Ji‐Woong , Woo Sang Uk , Kim Seong‐Taek , Liu Linhua , Lee Chul‐Hoon , Lee Seung Ki 
Published: November 2012

AbstractPhosphorylation‐dependent cytoplasmic translocation of human Cdc6 during S phase is sufficient to control its activity after origin firing. Export from the nucleus also serves as a mechanism for preventing re‐replication in mammalian cells. Phosphorylation of the CDK consensus serine residues 54, 74, and 106 has been suggested to be involved in the cytoplasmic translocation of Cdc6. To determine the relative importance of the three phosphorylation sites, we have generated Cdc6 variants by substituting one or more of the three serine residues with alanine or aspartic acid and have assessed their cytoplasmic translocation behavior. Phosphorylation of serine 74 mainly contributes to the cytoplasmic translocation of Cdc6, while serine 54 phosphorylation provides a minor contribution. In contrast, phosphorylation at serine 106 does not affect the nuclear export of Cdc6. Comparative results were found in cells coexpressing the phosphorylation defective mutants of Cdc6 and cyclin A as well as in non‐transfected cells synchronized by their release from a double thymidine block. We conclude that Cdk‐mediated phosphorylation of Cdc6 at serine 74 is required for the cytoplasmic translocalization of Cdc6 during the cell cycle. Phosphorylation of Cdc6 at serine 54 plays a minor role and phosphorylation of serine 106 plays no role in the cytoplasmic localization of Cdc6. The phosphorylation of S74 in Cdc6 could be important for binding to the nuclear export protein for translocalization. J. Cell. Physiol. 228: 1221–1228, 2013. © 2012 Wiley Periodicals, Inc.
Utilization of and charges for robotic versus laparoscopic versus open surgery for endometrial cancer
Authors: Yu Xinhua , Lum Deirdre , Kiet Tuyen K. , Fuh Katherine C. , Orr James , Brooks Rebecca A. , Ueda Stefanie M. , Chen Lee‐may , Kapp Daniel S. , Chan John K. 
Published: November 2012

AbstractBackground and ObjectivesTo analyze the utilization and hospital charges associated with robotic (RS) versus laparoscopic (LS) versus open surgery (OS) in endometrial cancer patients.MethodsHospital discharge data were extracted from Florida Agency for Health Care Administration between October 2008 and December 2009.ResultsOf 2,247 patients (median age: 64 years), 29% had RS, 10% had LS, and 61% had OS. The mean length of hospital stay was 1.6, 1.8, and 3.9 days for RS, LS, and OS, respectively (P < 0.001). The median hospital charge was $51,569, $37,202, and $36,492, for RS, LS, and OS (P < 0.001), with operating room charges ($22,600, $13,684, and $11,272) accounting for the major difference. Robotic surgery utilization increased by 11% (23–34%) over time.ConclusionsIn this statewide analysis of endometrial cancer patients, the utilization of robotic surgery increased and is associated with higher hospital charges compared to laparoscopic and open procedures. J. Surg. Oncol. 2013;107:653–658. © 2012 Wiley Periodicals, Inc.
Increased PD‐1 expression on CD4+ and CD8+ T cells is involved in immune evasion in gastric cancer
Authors: Saito Hiroaki , Kuroda Hirohiko , Matsunaga Tomoyuki , Osaki Tomohiro , Ikeguchi Masahide 
Published: November 2012

AbstractBackgroundCo‐signaling molecules play an important role in T cells. Programmed death‐1 (PD‐1) is an immunoinhibitory receptor and its overexpression on T cells appears to be involved in immune evasion in cancer patients. The present study was designed to investigate PD‐1 expression on T cells and its relationship with immune evasion in gastric cancer patients.MethodsPD‐1 expression on CD4+ and CD8+ T cells obtained from peripheral blood mononuclear cells (PBMC), normal gastric mucosa, and gastric cancer tissue was evaluated by multicolor flow cytometry.ResultsPD‐1 expression on CD4+ and CD8+ T cells from gastric cancer patients was significantly higher than that from normal controls. PD‐1 expression on CD4+ and CD8+ T cells was related to disease progression. Furthermore, PD‐1 expression on CD4+ and CD8+ T cells from gastric cancer tissue was significantly higher than that from normal gastric mucosa and PBMC. PD‐1 positive CD4+ and CD8+ T cells produced significantly less IFN‐gamma than PD‐1 negative CD4+ and CD8+ T cells.ConclusionsUpregulation of PD‐1 on both CD4+ and CD8+ T cells may be, in part, responsible for immune evasion in gastric cancer patients. J. Surg. Oncol. 2013;107:517–522. © 2012 Wiley Periodicals, Inc.
Salvage abdominoperineal resection for anal cancer following chemoradiation: A proposed scoring system for predicting postoperative survival
Authors: Correa Jose Humberto S. , Castro Leonaldson S. , Kesley Rubens , Dias Jurandir A. , Jesus Jose Paulo , Olivatto Luiz O. , Martins Ivanir O. , Lopasso Fabio P. 
Published: November 2012

AbstractBackground and ObjectivesAnal carcinoma is treated primarily by chemoradiation. Failure of this treatment requires salvage surgery. The aims of this retrospective study were to assess the survival probability after rescue surgery and design a pathological risk score (PRS) to predict postoperative outcome.MethodsFrom 1982 to 2011, the clinical and pathological data of 111 patients treated with chemoradiation or radiation alone and abdominoperineal resection were reviewed. The Kaplan–Meier method was used to assess overall survival and parametric modeling was applied to determine prognostic factors and design a PRS.ResultsThe 2‐ and 5‐year overall survival rates were 60% and 24.5%, respectively. The multivariate analysis showed that nodal disease (P < 0.03), resection margin (P < 0.001), and perineural and/or lymphovascular invasion (P < 0.0001) were significantly associated with survival. Patients who presented negative values for these three variables were estimated to show a 5‐year survival rate of 55% compared with 0.03% for patients who presented positive values.ConclusionsPositive surgical margin, the presence of perineural and/or lymphovascular invasion and positive nodal involvement were identified as significant independent predictors of mortality. The PRS was shown to be highly predictive of postoperative outcome. J. Surg. Oncol. 2013;107:486–492. © 2012 Wiley Periodicals, Inc.
Perioperative management of patients undergoing pancreatic resection: Implementation of a care plan in a tertiary‐care center
Authors: Salvia Roberto , Malleo Giuseppe , Butturini Giovanni , Dal Molin Marco , Esposito Alessandro , Marchegiani Giovanni , Paiella Salvatore , Malpaga Anna , Fontana Martina , Personi Beatrice , Bassi Claudio 
Published: November 2012

AbstractEvidence‐based perioperative management is important for a successful outcome after pancreatic surgery. Use of protocolized pathways of care based on fast‐track concepts has been shown to reduce hospital stay and contain costs. These regimens include pain control, early device removal (nasogastric tube, abdominal drains), enforced early mobilization, and early oral feeding. In this article, current evidence on perioperative management of pancreatic resections was analyzed in the attempt to implement our institutional care plan. J. Surg. Oncol. 2013;107:51–57. © 2012 Wiley Periodicals, Inc.
Blue LED inhibits the growth of Porphyromonas gingivalis by suppressing the expression of genes associated with DNA replication and cell division
Authors: Chui Chanthoeun , Hiratsuka Koichi , Aoki Akira , Takeuchi Yasuo , Abiko Yoshimitsu , Izumi Yuichi 
Published: November 2012

AbstractBackground and ObjectivesBlue light has been employed or investigated in both the medical and dental fields. Many studies have so far been reported a bactericidal effect of blue light emitting diodes (LED). However, it is still unclear whether exposure to blue LED kills or inhibits the growth of bacteria. We therefore investigated the effect of blue LED irradiation on the growth of Porphyromonas gingivalis compared with the effects of red LED.Materials and MethodsP. gingivalis cell suspensions were irradiated with blue or red LED (135 J/cm2) anaerobically, incubated for various lengths of time, and then the total RNAs were isolated. The RNA degradation and gene expression levels of stress‐related proteins in blue or red LED‐irradiated samples were examined using the RNA integrity number (RIN) and RT‐PCR, respectively. Quantitative RT‐PCR was done to investigate the gene expression profiles associated with chromosome replication and cell division.ResultsExposure to blue LED delayed the growth of P. gingivalis, while red LED did not. The RIN value indicated no RNA degradation in either the blue or red LED‐irradiated samples. In addition, the gene expression levels of stress‐related molecules remained either constant or increased 15 minutes after the blue LED irradiation compared to that before irradiation, thus suggesting that blue LED may not kill P. gingivalis cells. However, the blue LED irradiation did lead to a remarkably decreased expression of genes associated with chromosomal DNA replication and cell division after 5 minutes; exposure to the red LED did not.ConclusionThe inhibition of the growth of P. gingivalis by blue LED may therefore be induced not by a bactericidal effect, but instead due to a bacteriostatic effect mediated by the suppression of the genes associated with chromosomal DNA replication and cell division at the transcriptional level. Lasers Surg. Med. 44: 856–864, 2012. © 2012 Wiley Periodicals, Inc.
A case–control study on the association between rheumatoid arthritis and bladder pain syndrome/interstitial cystitis
Authors: Keller Joseph J. , Liu Shih‐Ping , Lin Herng‐Ching 
Published: November 2012

AbstractAimWhile bladder pain syndrome/interstitial cystitis (BPS/IC) has been suggested by a number of studies to have autoimmune character, no population‐based study to date has been conducted investigating its association with rheumatoid arthritis (RA). This study aimed to examine the association between IC/BPS and having previously been diagnosed with RA.MethodsWe conducted this study by using administrative claims data sourced from the Taiwan National Health Insurance Database. Our study included 9,269 cases with BPS/IC and 46,345 randomly selected controls. Conditional logistic regression was performed to calculate the odds ratio (OR) for the association between previously diagnosed RA and IC/BPS.ResultsRA was found among 202 (2.2%) cases and 504 (1.12%) controls. Conditional logistic regression analysis suggested that when compared with controls, the OR for prior RA among cases was 1.66 (95% CI = 1.47–1.87, P < 0.001) after adjusting for diabetes, hypertension, coronary heart disease, obesity, hyperlipidemia, chronic pelvic pain, irritable bowel syndrome, fibromyalgia, chronic fatigue syndrome, depression, panic disorder, migraine, sicca syndrome, allergy, endometriosis, asthma, overactive bladder, tobacco use disorder, and alcohol abuse. Additionally, BPS/IC was consistently and significantly associated with a previous diagnosis of RA regardless of prescription drug use; the OR for prior RA among groups prescribed ≤1 type of disease‐modifying antirheumatic drug (DMARD), two types of DMARDs, and ≥3 types of DMARDs or TNF‐alpha inhibitor when compared to controls were 1.49 (95% CI = 1.28–1.72), 1.91 (95% CI = 1.38–2.68), and 2.36 (95% CI = 1.77–3.17), respectively.ConclusionsThere is an association between RA and BPS/IC after adjusting for socio‐demographic characteristics and medical co‐morbidities. Neurourol. Urodynam. 32: 980–985, 2013. © 2012 Wiley Periodicals, Inc.
Biosimilars: Eine neue Gruppe rekombinanter Arzneimittel
Authors: Dingermann Theo , Zündorf Ilse 
Published: November 2012

AbstractSeit Mitte 2006 ist mit den Biosimilars eine völlig neue Gruppe von Arzneimitteln verfügbar (Abb. 1). Biosimilars sind molekulare Kopien patentfrei gewordener Biologika, die sich über viele Jahre im klinischen Alltag als wirksame und sichere Wirkstoffe zur Behandlung teils sehr schwerer Krankheiten bewährt haben. Obwohl man die Entwicklung hin zur Realisierung und Akzeptanz des Biosimilar‐Konzeptes lange für undenkbar gehalten hat, hat sich dieses Konzept nicht zuletzt auch Dank einer souverän agierenden Zulassungsbehörde durchgesetzt.
Die klinische Entwicklung von Biosimilars
Authors: Brockmeyer Carsten 
Published: November 2012

AbstractBiosimilare Arzneimittel (Biosimilars) sind Nachfolgeprodukte biomedizinischer Arzneimittel, die ihren Patentschutz verloren haben. Im Gegensatz zu “klassischen” Generika werden für Biosimilars in jedem Fall klinische Studien gefordert.
Patentrechtliche Aspekte bei der Herstellung von Biosimilars
Authors: Sommer Andrea 
Published: November 2012

AbstractBiosimilars sind Nachfolgeprodukte biotechnologischer Arzneimittel (Biopharmazeutika), deren Patentschutz abgelaufen ist. In der EU‐Gesetzgebung wurde der Begriff “einem biologischen Referenzarzneimittel ähnliches biologisches Arzneimittel” (Biosimilar) geprägt, da ein biopharmazeutisches Nachahmerpräparat nur ähnlich (similar), aber nicht identisch mit dem Originalpräparat sein kann.
Kommt bald die Pille für den Mann?
Authors: Zündorf Ilse , Dingermann Theo 
Published: November 2012

AbstractSeit den 1960er Jahren steht die Antibabypille zur oralen Kontrazeption für die Frau zur Verfügung. Dadurch wurde die Hauptverantwortung für die Familienplanung in die Hand der Frauen gelegt. Auch für den Mann wurde immer wieder – bisher mit wenig Erfolg – an einer “Pille” geforscht. Nun wurde eine Arbeit in Cell veröffentlicht, die einen wichtigen Schritt in die richtige Richtung aufzeigt.
Use of purified fibrinogen concentrate for dysfibrinogenemia and importance of laboratory fibrinogen activity measurement
Authors: Franzblau Emily B. , Punzalan Rowena C. , Friedman Kenneth D. , Roy Angshumoy , Bilen Ozlem , Flood Veronica H. 
Published: November 2012

AbstractWe report a patient with dysfibrinogenemia treated with purified fibrinogen concentrate who had discrepant post‐treatment laboratory values. The patient had mild bleeding symptoms and was diagnosed with dysfibrinogenemia based on fibrinogen activity of 51 mg/dl and antigen of 240 mg/dl. He was treated for an adenoidectomy with purified fibrinogen concentrate (RiaSTAP®) at a dose of 70 mg/kg. A discrepancy in post‐treatment fibrinogen activity was observed between the hospital and reference laboratories. Investigation revealed differences in laboratory assay and calibration methods. Fibrinogen concentrate may be a treatment option for patients with dysfibrinogenemia, but accurate laboratory technique is critical for fibrinogen measurement. Pediatr Blood Cancer 2013; 60: 500–502. © 2012 Wiley Periodicals, Inc.
Identifying periods of acceptable computer usage in primary care research databases
Authors: Horsfall Laura , Walters Kate , Petersen Irene 
Published: November 2012

ABSTRACTPurposeTo examine the effect of applying different data quality filters on the incidence of disease and prescribing trends over time in a primary care research database and validate a new method for defining periods of adequate computer usage.MethodsAcceptable computer usage (ACU) was defined as the year in which a general practice was continuously entering on average at least two therapy records, one medical record and one additional health data record per patient per year. The effect of using this date on the incidence of a range of outcomes (antibiotic prescriptions, myocardial infarction, colon cancer, lung cancer) over time was compared with other methods for defining the start of patient follow‐up in The Health Improvement Network (THIN) primary care database containing UK patient records. Various combinations of the follow‐up start dates were applied to the data to calculate incidence rates: (i) registration date, (ii) practice computerization date, (iii) acceptable mortality recording (AMR) (iv) ACU.ResultsOn average, the ACU date was 3.3 years after the AMR date. Applying the AMR or ACU dates separately or in combination produced trends in incidence rates more comparable with external data sources than using the year of practice registration or computerization. The estimated incidence rates were highly sensitive to different methods of defining start date in early time periods.ConclusionsUsing the latest of AMR and ACU dates is useful for improving the integrity of THIN data. Copyright © 2012 John Wiley & Sons, Ltd.
Controlling porous properties of polymer monoliths synthesized by photoinitiated polymerization
Authors: Yu Suzhu , Ma Khin Cho Cho , Mon Aye Aye , Ng Fern Lan , Ng Yen Yong 
Published: November 2012

AbstractPorous monoliths have been successfully prepared by photoinitiated polymerization of hydroxyethyl methacrylate and ethylene glycol dimethacrylate monomers in a porogenic solvent. By varying the composition of the starting solutions such as initiator fraction, monomer ratio and pore generator (porogen) fraction and type, as well as UV intensity, monoliths have been obtained with narrow pore size distribution and median pore size from about a few nanometres to a few micrometres. © 2012 Society of Chemical Industry
Lauftiere: vom Spielzeug zum Roboter
Authors: Ucke Christian , Schlichting Hans Joachim 
Published: November 2012

AbstractLauftiere wackeln eine schiefe Ebene hinunter oder werden durch ein sich absenkendes Gewichtsstück gezogen. Schon Kleinkinder sind fasziniert von diesem über hundert Jahre alten Spielzeug. Ingenieure beschäftigen sich aktuell damit, da sich mit dem dahinter stehenden Prinzip überraschend energiesparende Konstruktionen von Laufrobotern realisieren lassen. Eine genaue Analyse ergibt interessante Parallelen zur Stick‐and‐Slip‐Bewegung des Pickspechts.
Die Suche nach dem Unsichtbaren
Authors: Kiefer Michael 
Published: November 2012

AbstractViele astronomische Beobachtungsergebnisse belegen die Existenz von Dunkler Materie. Nach derzeitiger Erkenntnis besteht sie aus nichtbaryonischen, schwach wechselwirkenden Elementarteilchen (WIMPs), die das Standardmodell der Teilchenphysik nicht enthält. Sie sollten extrem geringe Wechselwirkungsquerschnitte besitzen, aber in so großer Zahl am Ort der Erde vorhanden sein, dass ein direkter Nachweis möglich erscheint. Das Experiment CRESST hat in seinen aktuellen Messdaten mehrere Ereignisse gefunden, die von WIMPs herrühren könnten. Letztliche Klarheit können jedoch nur weitere Daten bringen.
Der Radar‐Ritter
Authors: Loos Andreas 
Published: November 2012

AbstractBei seinem Tod ging ein Rauschen durch den Blätterwald. Schließlich war ein echter Ritter gestorben, der nicht nur wegen seiner Verdienste in der Radioastronomie in diesen Ehrenstand gekommen war: Als Radar‐Experte hatte er im Kalten Krieg an vorderster Front gestanden.
Suprafluidität in zwei Dimensionen
Authors: Weitenberg Christof 
Published: November 2012

AbstractSuprafluidität ist einer der verblüffendsten Effekte der Quantenmechanik. Dieser Zustand, bei dem sich eine Flüssigkeit ohne Reibung bewegt, wurde 1938 in flüssigem Helium entdeckt. Seit einigen Jahren studiert man ihn zudem intensiv an ultrakalten atomaren Quantengasen. Unserer Gruppe an der Ecole Normale Supérieure in Paris ist es kürzlich gelungen, die Supraflüssigkeit auch in einem zweidimensionalen Bose‐Gas direkt zu beobachten.
The Brush‐Shape Device Used to Generate Atmospheric and Homogeneous Plasmas for Biomedical Applications
Authors: Song Ying , Liu Dongping , Wang Wenchun , Ji Longfei , Zhang Qi , Ni Weiyuan , Niu Jinhai 
Published: November 2012

AbstractThe brush‐shape device mainly consisting of the well‐aligned and microns‐thick hollow quartz fibers can be used to generate an atmospheric and homogeneous cold plasma jet for biomedical applications. Addition of a trace amount of O2 into the feed gas greatly altered the electrical properties of the plasma jet, thus had an obvious effect on the sterilization efficiency. The uniformity of the brush‐shape plasma jet along its transverse direction was well consistent with the uniform distribution of sterilization efficiency. The atmospheric He plasma jet containing 1% O2 may kill all the Candida albicans cells with a population of 105 spores within an exposure time of 180 s. Analysis indicates that both O radicals and charged species can be an important contributor in this plasma inactivation. magnified image
Bronchial artery–pulmonary artery malformation as a cause of cryptogenic hemoptysis
Authors: Nugent Zoe , Oliveira Vicente , MacLusky Ian , Amaral Joao , Connolly Bairbre , Yang Connie L. 
Published: November 2012

AbstractA 12‐year‐old girl with two episodes of massive hemoptysis was found to have a localized pulmonary hemorrhage on bronchoscopy. Multiple investigations including a computed tomography (CT) angiogram failed to identify the cause. Catheterization of the pulmonary and bronchial vessels uncovered a bronchial artery to pulmonary artery fistula, which was embolized by interventional radiology. This is the first pediatric case of this type of anomaly and it illustrates the importance of angiography in the investigation of cryptogenic hemoptysis. Pediatr Pulmonol. 2013; 48:930–933. © 2012 Wiley Periodicals, Inc.