bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidermal Basement Membrane Alterations in Epidermolysis Bullosa Acquisita
Authors: Miyagawa Sachiko , Shirai Toshihiko , Matsumoto Hirohito , Komatsu Machiko , Iida Takashi , Sakamoto Kuniki 
Published: April 2015

AbstractImmunopathological studies on a patient with clinical evidence of epidermolysis bullosa acquisita (EBA) revealed an increase in type IV collagen in the skin of the chronic scarred lesion, but not in the clinically normal skin. These findings, together with multiplication of the lamina densa of the chronic lesional skin demonstrated by electron microscopy, strongly suggest that repeated injury possibly stimulates epidermal cells to secrete type IV collagen, leading to multiplication of lamina densa of the basement membrane at the dermal‐epidermal junction in EBA.
Cutaneous Angioma in Crow‐Fukase Syndrome: The Nature of Globules within the Endothelial Cells
Authors: Jitsukawa Kumiko , Hayashi Yoko , Sato Syozo , Anzai Takashi 
Published: April 2015

AbstractTo clarify the nature of the intracytoplasmic globules seen in the endothelial cells of the cutaneous angiomas of the Crow‐Fukase syndrome, we performed light and electron microscopic as well as immunohistochemical studies. The proliferating plump endothelial cells of the angioma located in the mid‐dermal portion contained numerous globules. IgG, A, M, and kappa and lambda light chain globulins were present in the endothelial cells containing these globules. Electron microscopy revealed osmiophilic fine fibrillar structures and amorphous substance in the globules. Thiéry staining for detection of vicinal‐glycol radicals of carbohydrate demonstrated the deposition of fine silver particles as a reaction product. We consider that the intracytoplasmic globules are phagolysosomes containing glycoprotein.
Phototoxic Index of Drug Determined by Candida Growth Inhibition
Authors: Horikawa Etsuro , Miura Takashi 
Published: April 2015

AbstractTo compare phototoxic potencies of drugs, an attempt was made to express them numerically. For this purpose, an in vitro phototoxic index was designed as the reciprocal of the product of minimal growth inhibition concentration and UVA dose in Candida, growth inhibition. Obtained results showed that the 11 drugs tested could be ranked by index as follows: 8‐methoxypsoralen (50.0) » prochlorperazine (5.0) > tiaprofenic acid (2.0) > chlorpromazine (1.25) > nalidixic acid (1.0) > naproxen (0.5) > fluorouracil (0.25)=ofloxacin (0.25) > enoxacin (0.2). Tegafur and piroxicam showed no inhibition.
Disseminated Superficial Actinic Porokeratosis with Sterile Spongiform Pustular Dermatitis
Authors: Dekio Satoshi , Jidoi Joli 
Published: April 2015

AbstractA 55‐year‐old woman with disseminated superficial actinic porokeratosis (DSAP) with sterile spongiform pustular dermatitis is described. The pustular dermatitis may have developed from friction of the DSAP lesions with clothes, gloves and/or stockings.
Effects of L‐glutamine on Tyrosinase and γ‐glutamyl Transpeptidase of B‐16 Melanoma Cells in Culture
Authors: Chakraborty Chandan , Hatta Susumu , Ichihashi Masamitsu , Hayashibe Kazuhito , Mishima Yutaka 
Published: April 2015

AbstractOmission of L‐glutamine (Gln) from the culture medium decreases tyrosinase activity and increases γ‐GTP activity in cultured melanotic B‐16 melanoma cells; inclusion of this amino acid in the medium shows the reverse effects. Its absence, however, decreases the γ‐GTP activity in non‐pigmented cells like HeLa cells, fibroblasts and amelanotic melanoma cells and its presence induces a dose dependent increase in this enzyme activity in these cells. The decrease of γ‐GTP activity in the melanotic melanoma cells by Gln is attributed to increased production of dopa and its derivatives by these cells, as evidenced by our results. Our data also indicate the inhibitory effects of dopa and several other dopa derivatives known to be produced by the melanoma cells on isolated bovine kidney γ‐GTP.
Fractionation of Epidermal Keratinocytes by Counterflow Centrifugal Elutriation (CCE)
Authors: Sakamoto Fumino , Nomura Hirofumi , Hachisuka Hiroshi , Sasai Yoichiro , Shiotsuki Kazuhide 
Published: April 2015

AbstractCounterflow centrifugal elutriation (CCE) is one of the most powerful and rapid methods for separating free cells by sedimentation velocity based on cell size, density, or shape. To obtain a sufficient number of cells, the fractionation of guinea pig epidermal keratinocytes was developed with the use of a newly‐developed elutriation system. An EDTA‐trypsin method was used to isolate keratinocytes; they were then separated into three groups according to flow rate by CCE. Morphologically, the keratinocytes in each region were identified as basal cells, spinous cells, and granular cells. The DNA distribution pattern agreed well with that obtained by Percoll's density gradient. The light‐scatter of keratinocytes in each fraction obtained by the present method was similar to that obtained with the density gradient method.
Sebaceous Carcinoma—With Special Reference to Histopathologic Differential Diagnosis
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Nishihara Kiyoshi , Imai Toshiya 
Published: April 2015

AbstractA case of incompletely differentiated sebaceous carcinoma and its histopathological findings were reported. In addition, morphological criteria for separating pseudo‐sebaceous tumors into categories were discussed.In this case, histopathological findings showed a mixture of basaloid and squamoid features. Specimens were examined with both light and electron microscopes.
Grouping Prurigo
Authors: Ofuji Shigeo , Ogino Atsuhiko 
Published: April 2015

AbstractThe skin disease we describe in this paper exhibits pruritic, inflammatory, solid papules that are grouped to form hen‐egg to goose‐egg‐sized, circumscribed lesions, usually on the trunk but occasionally on the extremities. The lesions do not spread in all directions but spread rather in only one or two, producing new papules while the old papules become flattened and leave pigmentation. After the lesions reach a size of a fist to a palm, or sometimes even larger, they begin to resolve. Usually a few or several lesions repeat remissions and exacerbations, taking a chronic course. We think that this disease constitutes a new clinical entity not yet reported.
Multiple Bowen's Disease Associated with Squamous Cell Carcinoma
Authors: Ismail Rokiah , Cheng Peh Suat 
Published: April 2015

AbstractThis paper reports a 50‐year‐old man with multiple Bowen's disease who subsequently developed squamous cell carcinoma.He had a possible history of chronic arsenical ingestion through drinking contaminated well water at a young age. The rain drop appearance of depigmented spots on the body and the warty hyperkeratosis on the palms and soles are manifestations of chronic arsenical ingestion.
Griseofulvin‐induced Photodermatitis
Authors: Kojima Takayuki , Hasegawa Takashi , Ishida Hisae , Fujita Masaru , Okamoto Shoji 
Published: April 2015

AbstractSix Japanese cases of griseofulvin (GF)‐induced photodermatitis were reported. All of the patients had received GF from 4 days to 4 months as treatment for tinea unguium. Clinical features were basically eczematous, but differed somewhat case by case. Patient 2 showed pigmentation and depigmentation and patient 5 showed pellagra‐like eruptions. From the clinical view, we could not differentiate precisely between phototoxic and photoallergic reactions. The difference may also depend on response severity, duration of skin eruption, or skin color. Blood and urine porphyrins were within normal ranges. An action spectrum study revealed that UVA was responsible for eliciting a photosensitivity reaction. Patch and photopatch tests were performed on all 6 patients. The results of the photopatch tests were positive for 3 patients and negative for the other 3. The degrees of reaction to photopatch tests differed so widely that it was difficult to determine suitable conditions for UVA intensity or GF concentrations for photopatch testing. Moreover, half of the patients showed negative photopatch tests. Although there is merit in the use of photopatch tests for studying the causes of systemically administered drug‐induced photodermatitis, there may also be limits to their usage. When performed carefully, drug readministration is a more reliable method. From the results of positive photopatch tests, photocross reactions between GF and penicillin, persistent light reactions and distant flare up phenomena, we concluded that in some of our cases, reactions were photoallergic. Histological findings showed non‐specific inflammatory changes. Direct immunofluorescence studies showed that immunoglobulin and complement were deposited at papillary perivascular areas and the dermo‐epidermal junction.
Polarization Assay Studies of Human Neutrophil Motility
Authors: Tsuda Shingo , Miyasato Minoru , Iryo Kayoko , Sasai Yoichiro 
Published: April 2015

AbstractThe polarization assay was used to assess the effects of chemoattractants on neutrophil motility by means of cellular configuration. The addition of N‐formyl‐L‐methionyl‐phenylalanine (f‐Met‐Phe) to a neutrophil suspension from human peripheral blood induced a polarized change in cellular configuration, and the ratio of such transfiguration reached a maximum at 10–8 to 10–6 M of f‐Met‐Phe. The polarized configuration appeared 1 min after addition of f‐Met‐Phe and showed its maximum response at 10 min. The number of polarized cells were decreased after exposure to cytochalasin B or incubating with corticosteroid. The increased response of random polarization was induced in a dose‐dependent fashion by ascorbic acid. Tetracycline HCl did not cause any changes in cell shape at any concentrations examined. This assay can be used to examine patients with depressed neutrophil motility, such as those with systemic lupus erythematosus in the active phase and a severe case of atopic dermatitis.
Administration of IgG Fraction of Epidermolysis Bullosa Acquisita (EBA) Serum into Mice
Authors: Shigemoto Takashi , Nashiro Kiyoko , Tsuchida Tetsuya , Seki Yoshihito , Tamaki Kunihiko 
Published: April 2015

AbstractIn order to study the pathogenic role of autoantibodies in the serum of EBA patients, we tried to induce EBA lesions in mice by administration of the IgG fraction of EBA serum via the intraperitoneal (i.p.) or subcutaneous (s.c.) routes into neonatal or adult mice. We also tried to induce EBA lesions in fetuses by intravenous (i.v.) injection of EBA serum into pregnant mice.This study demonstrates that IgG fraction of EBA serum injected into neonatal or adult mice, but not into fetal mice, deposits at the epidermo‐dermal junction in a linear fashion but fails to induce bullae. The role of EBA serum in the pathogenesis of EBA lesions is discussed.
Autoantibodies to Vimentin‐type Intermediate Filament in Patients with Progressive Systemic Sclerosis
Authors: Tsuneda Yoriko , Kitajima Yasuo , Mori Shunji 
Published: April 2015

AbstractUsing immunoblotting techniques, sera from patients with systemic lupus erythematosus (SLE) and progressive systemic sclerosis (PSS) and from normal individuals were tested for the presence of antibodies to the intermediate filament protein (vimentin) of the cytoskeleton of human fibroblasts. Antibodies to vimentin were found in sera from both patients and normal controls. By the enzyme‐linked immunosorbent assay (ELISA) using vimentin purified from cultured human skin fibroblasts, we determined the titer of the antibodies in PSS (50 cases), and SLE (8 cases), and normal sera (48 cases). Significantly higher titers of anti‐vimentin auto‐antibodies were detected in the patient sera than in normal controls. According to the titers, the cases of PSS were divided into two groups: one with titers higher than the range of normal controls, and another with titers within the range of normal controls. The relationships of the titers to the clinical features were examined. No significant correlations between anti‐vimentin titer and clinical characteristics were found with regard to sex, onset‐age, duration of the disease, lung involvement, proximal scleroderma, or titers of anti‐nuclear, anti‐RNP, anti‐Scl‐70, anti‐centromere and anti‐DNA‐antibodies.
A Trial of Acupuncture for Progressive Systemic Sclerosis
Authors: Maeda Manabu , Ichiki Yoshiro , Sumi Atsushi , Mori Shunji 
Published: April 2015

AbstractThirty four cases of PSS were treated once or twice a week by a low frequency electrical acupuncture method for periods of 3 months to 9 years and 2 months. On clinically worse side of arm, two meredian points, Hatija and Shokai , were used for the electrical stimulation. As the result, various degrees of improvement were obtained in ulcerations on the fingertips or toetips, arthralgia, cutaneous sclerosis, hypo‐ and hyperpigmentation and sensory disturbance etc. and in respiratory functions, plethysmography, capillary microscopic features of the nail bed and histopathologic features biopsied from the forearms. Laboratory data showed remarkable improvement in the inflammatory reactions. However, the improvement in immune reactions was poor paralleling the poorness of clinical improvement and prognosis.
Case Report and Study of Collagen Metabolism in Ehlers‐Danlos Syndrome Type II
Authors: Kojima Takayuki , Shinkai Hiroshi , Fujita Masaru , Morita Eiji , Okamoto Shoji 
Published: April 2015

AbstractA case report on a 41‐year‐old Japanese woman with prominent features of Ehlers‐Danlos syndrome is presented.At birth, the patient was noted to have moderate hyperlastic skin and hypermobility of the joints, especially those of the hands and fingers. She bruised easily, formed purpura, and had cigarette paper scars. From the age of 38, she became aware of palpitation, dyspnea and easy fatigue on exertion; examination showed mitral insufficiency. Mitral‐valve prolapse was revealed by echocardiogram and CT echogram. Upper G‐I series of X‐ray revealed duodenum diverticula. At the age of 42, she was operated for mitral valve replacement. From the clinical features she was dignosed as having Ehlers‐Danlos syndrome type II. Tissue sections stained with H‐E showed a few, fine, disorderly arranged collagen fibers. Electron microscopically, the diameter of individual collagen fibrils was rather more varied than that in normal skin, but the average diameter was within the normal range. In longitudinal sections, no apparent abnormal findings could be observed.Cultured dermal fibroblasts from the patient synthesized pro alpha 1 trimer of type I collagen but failed to secrete type III procollagen or pro alpha 2 chain of type I collagen. Affected cells were stained with antibodies against type I and type III collagen. Specific fluorescences against type I collagen were observed and very faint positive results against type III collagen were seen. These studies indicate that secreted altered type I procollagens that are synthesized can result in the clinical syndrome of our case.
Pigmented Neurofibroma
Authors: Jurecka Wolfgang 
Published: April 2015

AbstractA 24‐year‐old male patient suffering from a large‐coconut‐sized neurofibroma is described. In the tumor, sharply demarcated areas of melanin containing cells were seen. These cells were identified as melanin producers by the premelanosomes seen by electron microscope. Although the coexistence of neurofibroma with another melanin‐producing tumor, such as cellular blue nevus, could not be ruled out, this tumor shows the close relationship between tumors of the peripheral nervous system and melanocytic malformations or pigmented nevi. These observations are relevant to the controversy over the histiogenesis of these pigmented lesions and support Masson's view that nerve sheath cells are capable of melanogenesis.
Haematoxylin Stainable Epidermal Protein of the Newborn Rat
Authors: Takahashi Masae , Tezuka Tadashi 
Published: April 2015

AbstractA polyclonal antibody elicited by a newly purified haematoxylin stainable protein (HSP) from newborn rat epidermis by Superose 12 column chromatography and Mono Q anion‐exchange column chromatography showed localization in the cell membrane region of the stratum corneum cells of the flank skin and the foot pad skin of newborn rats, but not of adult rats in indirect immunofluorescent studies. In addition, although the stratum corneum of neither the fetal flank nor the foot pad skin (20th day of gestation) reacted with the antibody, both of them became positive shortly after birth (5 hours). However, the positive areas in the stratum corneum of the foot pad skin gradually moved to the upper part. Only the upper one third was positive at the 15th day after birth and the entire stratum corneum finally became negative in the adult. By immunoblotting technique, antigenic materials were detected in the fetal flank and foot pad skins, and in the adult foot pad skin, but not in the flank skin of adults. In immunofluorescent studies, neither the cornified nor non‐cornified epithelia of the oral membrane, tongue, esophagus, trachea or bladder of the newborn rats (3‐days old) reacted with this antibody. In studies of the reactivity of the stratum corneum of the other species, the outer one third of the stratum corneum of the flank skin of newborn mice positively reacted. However, the fluorescence was not membrane‐located, but rather diffuse. Newborn guinea pig epidermis showed no positive reaction. The epidermis of adult mice, guinea pigs, dogs and humans showed no reaction with this antibody.
Expression of Transferrin Receptor in Normal Human Skin, Psoriatic Skin and Various Skin Tumors
Authors: Iwata Mitsuru , Otsuka Fujio , Watanabe Shin‐ichi , Nogita Tatsutoshi , Ishibashi Yasumasa 
Published: April 2015

AbstractThe distribution of transferrin receptor in normal human skin and its expression in psoriatic skin and various skin tumors have been investigated. Immuno‐peroxidase staining was performed on biopsy specimens using monoclonal OKT 9 antibody, which reacts with transferrin receptor.Normal human skin showed positive staining with OKT 9 in eccrine glands and outer root sheaths of the hair. Sebaceous glands were also strongly positive. The basal layer stained very weakly.Psoriatic skin expressed OKT 9 strongly in the epidermis, especially in the area of the rete ridge.In squamous cell carcinoma, Bowen's disease, and malignant melanoma, a widespread and strong labelling reaction was found. Basal cell epithelioma and genital Paget's disease were partially and moderately positive in their staining pattern. No such positive staining pattern could be found in either nevus cell nevi or seborrhoic keratosis.These findings indicate that, in normal skin, transferrin receptor exists in eccrine glands, sebaceous glands, and outer root sheaths of the hair in greater amounts. High amounts of expression of this receptor in psoriatic epidermis and malignant tumors suggests that immunohistochemical demonstration of transferrin receptor parallels the proliferating activity of the tissue or tumor of the skin and may provide an useful aid for detecting such conditions.
A Case of Dermatomyositis and Hashimoto's Thyroiditis
Authors: Kato Hideyuki , Uyeki Yumiko , Kitajima Yasuo , Yaoita Hideo 
Published: April 2015

AbstractWe report a 59‐year‐old female patient with dermatomyositis associated with Hashimoto's thyroiditis. It is generally agreed that overlap of autoimmune diseases occurs during their course. The relationship between dermatomyositis and Hashimoto's thyroiditis is discussed in terms of autoimmune diseases, and several similar cases in the literature are reviewed.
Epidermal Factors Stimulate Collagenase Synthesis by Epidermolysis Bullosa Fibroblasts
Authors: Nomura Kazuo , Takahashi Masaaki , Mikami Hideki , Imaizumi Takashi , Sawada Yukimasa , Hashimoto Isao 
Published: April 2015

AbstractThe effect of epidermal factors on the collagenase activity of epidermolysis bullosa (EB) fibroblasts was examined. The epidermal extract obtained from a surgical scar strikingly stimulated the collagenase activity by fibroblasts to 2.55–3.07 U/ml (EB dystrophica recessiva, EBDR) and 1.67–2.03 U/ml (EB dystrophica dominans, EBDD), compared with 0.02–0.07 U/ml (EBDR) and 0–0.04 U/ml (EBDD) in the absence of the factor. Murine epidermal cell‐conditioned medium also enhanced the enzyme activity to 1.37–1.41 U/ml (EBDR) and 0.30–0.94 U/ml (EBDD). These results indicate that the epidermal factors, possibly interleukin‐1, may regulate collagenase metabolism in EB. Interleukin‐1‐positive cells were also observed immunohistochemically in the epidermis of EB. Therefore, the present data support the possibility that epidermis may be directly or indirectly involved in altering collagenase synthesis by EB fibroblasts.
Conserved Gene Organization of 67K Keratin in Patients with Hereditary Palmoplantar Keratoderma
Authors: Tanaka Toshihiro , Horiguchi Yuji , Moriwaki Shin‐ichi , Danno Kiichiro , Ikai Kouichi , Imamura Sadao 
Published: April 2015

AbstractRestriction enzyme analysis to determine the divergence of the gene organization and the restriction fragment length polymorphism in four patients with two types of hereditary palmoplantar keratoderma revealed that the gene organization of 67K keratin was conserved in all cases. No restriction enzyme fragement length polymorphism was observed in a family of the Vörner type (Epidermolytic hereditary palmoplantar keratoderma) nor in a family of the Unna‐Thost type.Total southern blotting analysis of both patients and controls showed the same pattern of bands; digestion with BamHI revealed 16.5 Kilo base pairs (Kbp) and 8.5 Kbp, whereas double digestion with BamHI and EcoRI showed bands of 7.2 Kbp and 4.5 Kbp, and that with BamHI and SphI gave 6.0 Kbp and 1.7 Kbp.These results indicate that abnormalities in the keratin pattern, especially a decrease in the amount of higher molecular weight keratin in Vörner type palmoplantar hyperkeratosis, are not due to changes in the gene organization, such as insertion and/or deletion in the 67K keratin gene, and that there is no polymorphism around the 67K keratin gene of either type.
Low‐molecular‐weight Leukocyte Chemotactic Factors in Psoriatic Scales: Contribution of Lipid‐soluble Factors to the Chemotactic Activity
Authors: Takematsu Hideaki , Kato Taizo , Tagami Hachiro 
Published: April 2015

AbstractPsoriatic scales contain polymorphonuclear leukocyte (PMN) chemotactic factors which presumably are responsible for transepidermal PMN chemotaxis in psoriatic lesions. On molecular sieve chromatography, psoriatic scale extracts revealed PMN chemotactic activity in the peptide fractions eluted near 12 kDa in addition to low molecular weight (MW) chemotactic fractions. PMN chemotactic peptides with MW around 12 kDa that contain a C5‐cleavage product have been characterized as a unique major chemotactic factor in the aqueous components. On the other hand, it is uncertain whether or not lipid‐soluble low MW chemotactic factors, i.e., leukotriene B4 (LTB4) and its related compound 12‐hydroxy‐5,8,10,14‐eicosatetraenoic acid (12‐HETE), play major roles as PMN chemoattractants. In the present study, we found that the chemotactic activity in the aqueous psoriatic extracts was much higher than that in the organic extracts. Chemotactic activity in the low MW chemotactic fractions of psoriatic scale extracts, about half of which was recovered after extraction with ether at acidic pH, was heat‐stable (100°C) and withstood protease digestion, but was partially inactivated by treatment with trypsin. Immunoreactive LTB4 was demonstrated in these fractions. The previous findings and the present results suggest that the lipid‐soluble chemotactic factors, LTB4 and 12‐HETE, which constitute the low MW fraction of the psoriatic scale extracts, do not play as major a role as a PMN chemoattractants as do the chemotactic peptide fractions.