bluuebunny/crossref_metadata_2025_split huggingface dataset:
Metabolomic analysis of Agkistrodon haly venom poisoning mouse treatment by Jidesheng snake pill based on GC-MS
Authors: Luo Jie , Guo Minkang , Xie Ke , Han Ting-Li , Ai Shanmu 
Published: August 2024

IntroductionSnakebites are acute systemic toxic diseases caused by snake venom entering the body through wounds. Failure to use antivenom immediately and difficulty in obtaining antivenoms are frequently responsible for worsening disease. Traditional Chinese medicine is commonly used to supplement and replace antivenom in treating snakebites. The Jidesheng snake pill (JDS) is a widely used traditional Chinese medicine that has achieved good clinical therapeutic effects; however, its mechanism remains unclear. Therefore, metabolomics techniques were employed to explore the pathophysiological mechanisms of JDS treatment of Agkistrodon halys (Ah) snake venom-poisoned mice.MethodsThe Ah group mouse model was established by intramuscular injection of Ah venom into the hind legs of the mice. The Ah venom + JDS group model was established using JDS after the affected area was treated with Ah venom. Hematoxylin and eosin (HE) staining was used to evaluate the severity of gastrocnemius injury. Quantitative polymerase chain reaction (qPCR) was utilized to detect the mRNA expression of vascular cell adhesion molecule-1 (VCAM-1), muscle-specific creatine kinase (CKM), thrombin antithrombin complex (TAT), and tumor necrosis factor-alpha (TNF-α). Gas chromatography-mass spectrometry (GC-MS) was performed with multivariate statistical analysis to provide new insights into the global metabolic profile of Ah venom-poisoned mice.ResultsHE staining revealed increased red cell necrosis, local hemorrhage, and neutrophil infiltration in the Ah venom group than in the control group. Several compounds were identified, including lipids, amino acids, peptides, and organooxygen. Eighty differential metabolites were screened between the control group and the Ah venom group, and 24 were screened between the Ah venom and JDS groups. The mechanism of Ah venom poisoning in mice may involve aminoacyl-tRNA biosynthesis, various amino acid metabolism disorders, tricarboxylic acid circulation disorders, and abnormal fatty acid metabolism. JDS may reduce symptoms by affecting long-chain fatty acid and amino acid metabolism and promoting nicotinamide-nicotinamide metabolism.ConclusionOur results suggest that metabolomics has huge prospects for elucidating the pathophysiology of Agkistrodon haly venom poisoning and therapeutic mechanisms of JDS.
The effect of convalescent plasma therapy on the rate of nucleic acid negative conversion in patients with persistent COVID-19 test positivity
Authors: Wang Yixuan , Xu Zhe , Xu Xue , Yang Shuwen , Li Yuanyuan , Zhang Hanwen , Zhang Yufeng , Wang Fu-Sheng , Wang Ying , Bi Jingfeng 
Published: August 2024

ObjectiveThis study investigates the association between convalescent plasma therapy and the negative conversion rate in patients with persistent COVID-19 test positivity.MethodA retrospective analysis was conducted on patients with severe or mild to moderate COVID-19 whose viral nucleic acid tests remained positive for over 30 days. Patients were categorized into two groups: those who administered convalescent plasma therapy and those who were not. Data collected included information on therapy strategies used (convalescent plasma, corticosteroids, interferons, etc.), patients’ demographic characteristics, comorbidities, therapeutic medications, and nucleic acid testing results. Patients in the convalescent plasma therapy group were matched 1:2 ratio with those in the non-convalescent plasma therapy group. Cumulative negative conversion rates on the fifth, tenth, and fifteenth days post-therapy initiation were analyzed as dependent variables. Independent variables included therapy strategies, demographic characteristics, comorbidities, and therapeutic medication usage. Univariate analysis was conducted, and factors with a p-value (P) less than 0.2 were included in a paired Cox proportional hazards model.ResultsThere was no statistically significant difference in the cumulative negative conversion rate between the convalescent plasma therapy group and the non-convalescent plasma therapy group on the fifth, tenth, and fifteenth days. Specifically, on day the fifth, the negative conversion rate was 41.46% in the convalescent plasma therapy group compared to 34.15% in the non-convalescent plasma therapy group (HR: 1.72, 95% CI: 0.82–3.61, P = 0.15). On the tenth day, it was 63.41% in the convalescent plasma therapy group and 63.41% in the non-convalescent plasma therapy group (HR: 1.25, 95% CI: 0.69∼2.26, P = 0.46). On the fifteenth day, the negative conversion rate was 85.37% in the convalescent plasma therapy group and 75.61% in the non-convalescent plasma therapy group (HR: 1.19, 95% CI: 0.71–1.97, P = 0.51).ConclusionOur finding does not support the hypothesis that convalescent plasma therapy could accelerate the time to negative conversion in patients who consistently test positive for COVID-19.
Toxic epidermal necrolysis caused by phenobarbital: a case report and literature review
Authors: Cheng Jie , Li Hui , Li Yan , Li Xiao , Wang Jianjun , Huang Xin , Cui XueYan 
Published: August 2024

BackgroundToxic epidermal necrolysis (TEN) and Stevens-Johnson syndrome (SJS) are rare, life-threatening immunologic reactions. Previous relevant literature has provided limited information regarding this disease’s genetic susceptibility and management principles.ObjectivesThis study aimed to describe a phenobarbital-induced TEN case report with HLA-B*15:02 and HLA-B*58:01 negative, CYP2C19*1/*2. In addition, we revised the existing literature on phenobarbital-induced SJS/TEN to explore its clinical characteristics.MethodsWe describe a woman undergoing treatment with Phenobarbital for status epilepticus who developed classic cutaneous findings of TEN. A systematic search was conducted in the PubMed, Medline, WanFang, and CNKI databases from 1995 to 2023. The search terms used were “Stevens-Johnson Syndrome,” “Toxic Epidermal Necrolysis,” and “Phenobarbital.”ResultsWe report a case of TEN resulting from phenobarbital; it tested negative for the HLA-B*15:02 and HLA-B*58:01 allele and CYP2C19*1/*2 intermediate metabolism. Supportive treatment with steroids and antihistamines resulted in complete resolution of the skin lesions and improvement in clinical symptoms after 14 days. Physicians and clinical pharmacists should be aware of these potential phenobarbital-related adverse events and closely monitor patients with first-time use of phenobarbital. Among 19 cases were identified in the literature, with 11 (57.9%) cases of SJS, 6 (31.6%) cases of TEN, and 2 (7.2%) cases of SJS-TEN/DRESS overlap. A total of 5 (26.3%) did not survive, of which 4 (21.1%) were under 12 years old and 1 (5.3%) was over 12 years old.ConclusionPhenobarbital-induced SJS/TEN may still occur in patients who test negative for HLA-B*15:02 and HLA-B*58:01, CYP2C19*1/*2. Most cutaneous adverse events occur early in the course of Phenobarbital therapy and should be closely monitored early in the course of treatment. In addition, Phenobarbital should be used with caution in patients with a history of asthma and allergy to antipyretics and analgesics.
Quantitative analysis of systemic perfusion and cerebral blood flow in the modeling of aging and orthostatic hypotension
Authors: Cheng Heming , Dai Jifeng , Li Gen , Ding Dongfang , Li Jianyun , Zhang Ke , Wei Liuchuang , Hou Jie 
Published: August 2024

Introduction: Orthostatic hypotension (OH) is common among the older population. The mechanism hypothesized by OH as a risk factor for cognitive decline and dementia is repeated transient cerebral blood flow deficiency. However, to our knowledge, quantitative evaluation of cardiac output and cerebral blood flow due to acute blood pressure changes resulting from postural changes is rare.Methods: We report a new fluid-structure interaction model to analyze the quantitative relationship of cerebral blood flow during OH episodes. A device was designed to simulate the aging of blood vessels.Results and Discussion: The results showed that OH was associated with decreased transient cerebral blood flow. With the arterial aging, lesions, the reduction in cerebral blood flow is accelerated. These findings suggest that systolic blood pressure regulation is more strongly associated with cerebral blood flow than diastolic blood pressure, and that more severe OH carries a greater risk of dementia. The model containing multiple risk factors could apply to analyze and predict for individual patients. This study could explain the hypothesis that transient cerebral blood flow deficiency in recurrent OH is associated with cognitive decline and dementia.
Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome
Authors: Su Hang , Wang Yuanchun , Xu Jin , Omar Ahmad A. , Grosser Jude W. , Wang Nian 
Published: August 2024

IntroductionCitrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a devastating disease worldwide. Previously, we successfully generated canker-resistant Citrus sinensis cv. Hamlin lines in the T0 generation. This was achieved through the transformation of embryogenic protoplasts using the ribonucleoprotein (RNP) containing Cas12a and one crRNA to edit the canker susceptibility gene, CsLOB1, which led to small indels.MethodsHere, we transformed embryogenic protoplasts of Hamlin with RNP containing Cas12a and three crRNAs.ResultsAmong the 10 transgene-free genome-edited lines, long deletions were obtained in five lines. Additionally, inversions were observed in three of the five edited lines with long deletions, but not in any edited lines with short indel mutations, suggesting long deletions maybe required for inversions. Biallelic mutations were observed for each of the three target sites in four of the 10 edited lines when three crRNAs were used, demonstrating that transformation of embryogenic citrus protoplasts with Cas12a and three crRNAs RNP can be very efficient for multiplex editing. Our analysis revealed the absence of off-target mutations in the edited lines. These cslob1 mutant lines were canker- resistant and no canker symptoms were observed after inoculation with Xcc and Xcc growth was significantly reduced in the cslob1 mutant lines compared to the wild type plants.DiscussionTaken together, RNP (Cas12a and three crRNAs) transformation of embryogenic protoplasts of citrus provides a promising solution for transgene-free multiplex genome editing with high efficiency and for deletion of long fragments.
The influence of adolescents’ self-perception of social relationships on personality functioning in the context of inclusive education
Authors: Hartmann Anne , Knigge Michel , Lenkeit Jenny , Ehlert Antje , Goth Kirstin , Spörer Nadine 
Published: August 2024

IntroductionAdolescence is a special phase of life in which fundamentals of psychosocial functioning are formed. The present study investigates how adolescents’ self-perception of social relationships in inclusive classes affect personality functioning. Furthermore, we examine whether the association between self-perceived social relationships and personality functioning is stronger for students with special educational needs in the domains of learning (SEN L) and social -emotional development (SED) than for students without SEN.MethodsAt two measurement points questionnaire data was collected from 927 sixth- and seventh-graders from 20 primary and 20 comprehensive inclusive classes in Germany.ResultsResults of longitudinal multilevel analyses show partially different results for sixth- and seventh-graders. Overall, students’ perceived social relatedness predicted personality functioning. Students who perceived their social relationships more positively showed healthier personality functioning. Further, SEN SED represents a potential risk factor for personality functioning. But, we observed that differences in personality functioning between seventh-graders with SEN L or SED and those without SEN decreased over time. Furthermore, SEN does not appear to reinforce the association between low self-perception of social relationships and risky personality functioning.DiscussionThe findings are discussed in the context of inclusive education and implications for future research as well as practice are given. Overall, our findings contribute to a better understanding of students’ psychosocial development in inclusive learning environments.
Unmet healthcare needs and the local extinction index: an analysis of regional disparities impacting South Korea’s older adults
Authors: Kwon Younggyu , Sohn Minsung , Choi Mankyu 
Published: August 2024

BackgroundThis study examines the factors affecting unmet healthcare experiences by integrating individual-and community-level extinction indices.MethodsUsing spatial autocorrelation and multilevel modeling, the study utilizes data from the Community Health Survey and Statistics Korea for 218 local government regions from 2018 to 2019.ResultsThe analysis identifies significant clustering, particularly in non-metropolitan regions with a higher local extinction index. At the individual level, some factors affect unmet medical needs, and unmet healthcare needs increase as the local extinction index at the community level increases.ConclusionThe findings underscore the need for strategic efforts to enhance regional healthcare accessibility, particularly for vulnerable populations and local infrastructure development.
Genomic Insights into Bacillus thuringiensis V-CO3.3: Unveiling Its Genetic Potential against Nematodes
Authors: Palma Leopoldo , Bel Yolanda , Escriche Baltasar 
Published: August 2024

Bacillus thuringiensis (Bt) is a Gram-positive, spore-forming, and ubiquitous bacterium harboring plasmids encoding a variety of proteins with insecticidal activity, but also with activity against nematodes. The aim of this work was to perform the genome sequencing and analysis of a native Bt strain showing bipyramidal parasporal crystals and designated V-CO3.3, which was isolated from the dust of a grain storehouse in Córdoba (Spain). Its genome comprised 99 high-quality assembled contigs accounting for a total size of 5.2 Mb and 35.1% G + C. Phylogenetic analyses suggested that this strain should be renamed as Bacillus cereus s.s. biovar Thuringiensis. Gene annotation revealed a total of 5495 genes, among which, 1 was identified as encoding a Cry5Ba homolog protein with well-documented toxicity against nematodes. These results suggest that this Bt strain has interesting potential for nematode biocontrol.
Bioinformatics Analysis of the Genome of Rhodococcus rhodochrous IEGM 1362, an (−)-Isopulegol Biotransformer
Authors: Maltseva Polina Yu. , Plotnitskaya Natalia A. , Krivoruchko Anastasiia V. , Beletskiy Aleksey V. , Rakitin Andrey L. , Mardanov Andrey V. , Ivshina Irina B. 
Published: August 2024

A genome of Rhodococcus rhodochrous IEGM 1362 was sequenced and annotated. This strain can transform monoterpene alcohol (–)-isopulegol with the formation of two novel pharmacologically promising metabolites. Nine genes encoding cytochrome P450, presumably involved in (–)-isopulegol transformation, were found in the genome of R. rhodochrous IEGM 1362. Primers and PCR conditions for their detection were selected. The obtained data can be used for the further investigation of genes encoding enzymes involved in monoterpene biotransformation.
Enhancing Point-of-Use Sand Filters through Integration of Copper Mesh
Authors: Kasaraneni Varun K. , Anaya Nelson M. , Taliani Maria 
Published: August 2024

Ensuring access to safe drinking water remains a crucial global challenge, particularly in low-income and developing regions. Point-of-use (POU) sand filters, known for their affordability and efficiency, hold promise for improving water quality. This study explores the use of copper mesh (M-Cu) as a disinfectant in sand filters, focusing on its effectiveness against Escherichia coli (E. coli). Through a series of experiments—kinetic, batch, and column—we investigated the impact of M-Cu on bacterial inactivation. Our findings reveal that the contact time between the M-Cu and bacteria is more critical than the M-Cu mass for achieving significant microbial reduction. Specifically, 1.0 g of M-Cu can substantially reduce E. coli levels, achieving log removal values (LRVs) between 2.03 and 4.81 after 30 min of exposure, across initial bacterial concentrations ranging from 102 to 106 CFU/100 mL. For sand filter testing under dynamic conditions, columns containing 1.0 g of M-Cu achieved a 3.1 LRV, significantly outperforming control columns, which only achieved a 0.6 LRV. These results support the integration of M-Cu into sand filters as a viable strategy for enhancing microbial safety in water treatment, potentially reducing waterborne disease incidence in vulnerable populations.
ROTAVIRAL AND CORONAVIRAL INFECTIONS OF CATTLE IN THE IRKUTSK REGION
Authors: Батомункуев Алдар Содномишиевич , Евдокимов Петр Иванович , Мельцов Иван Владимирович 
Published: August 2024

Рота- и коронавирусные инфекции занимают одно из ведущих мест в патологии желудочно-кишечного тракта телят. В статье представлен анализ распространенности рота- и коронавирусной инфекций крупного рогатого скота на территории Иркутской области, проведенный по отчетным формам Иркутской межобластной ветеринарной лаборатории за период2004-2017 гг. согласно результатам лабораторной диагностики. Установлено, что в Иркутской области антиген ротавируса у телят профилакторного возраста методом иммуноферментного анализа выявлен в 17,6±2,3% случаевв шести районах (Иркутский, Шелеховский, Боханский, Черемховский, Заларинский, Зиминский), при этом основная доля положительных проб установлена в Иркутском районе (56,8%). Геном вируса ротавирусной инфекции выявлен методом полимеразной цепной реакции в 17,8±2,4% от числа исследованных проб; все случаи диагностированы у телят в Усольском районе.Антиген коронавируса в смывах с прямой кишки новорожденных телят методом реакции гемагглютинации получен в 2,2±0,9% проб в трех районах (Иркутский, Боханский, Эхирит-Булагатский) с доминированием Иркутского района (60,0%).При исследовании фекалий, взятых от телят (n = 99) в возрасте от 1 суток до 3 месяцев в трех хозяйствах Иркутской области, в 44,4% случаев выделяли антиген ротавируса и в 11,1% - коронавируса.
Rota- and coronavirus infections lead in the pathology of the gastrointestinal tract of calves. The analysis of the prevalence of rota- and coronavirus infections of cattle in the Irkutsk region is presented in the article. The analysis was carried out according to the data studies based on the results of laboratory diagnostics provided by the Irkutsk Interregional Veterinary Laboratory for years of 2004-2017. The antigen of rotavirus in the calves of the prophylactic age by the method of enzyme-linked immunosorbent assay was detected in 17,6±2,3% of cases in six districts of the Irkutsk region (Irkutskу, Shelekhovsky, Bokhansky, Cheremkhovsky, Zalarinsky, Ziminsky); the majority of positive samples was collected in the Irkutskу districts (56,8%). The genome of rotavirus infection virus was detected by Polymerase Chain Reaction in 17,8 ± 2,4% of the number of samples examined; all cases are diagnosed in calves in Usolsky district.The antigen of coronavirus was obtained from flushing of newborn calves by the method of reaction of haemagglutination in 2,2 ± 0,9% of samples taken in three districts (Irkutskу, Bokhansky, Ehirit-Bulagatsky; the Irkutsky districts dominated (60,0%). In the study of faeces taken from calves (n = 99) aged 1 day to 3 months in three farms of the Irkutsk region, in 44.4% of cases rotavirus antigen was isolated and in 11.1% - coronavirus.
ANALYSIS OF BEEF CATTLE BREEDING IN THE URAL FEDERAL DISTRICT OF THE RUSSIAN FEDERATION
Authors: Бахарев Алексей Александрович , Литкевич Антон Иванович , Бугасов Беимбет Жасуланович 
Published: August 2024

В статье представлено аналитическое состояние отрасли специализированного мясного скотоводства в Уральском федеральном округе Российской Федерации - Челябинской, Тюменской (без автономных округов), Свердловской, Курганской областей, структура и породный состав поголовья. Исследования представлены за период с 2016 по 2018 год. Так, наибольший прирост поголовья крупного рогатого скота специализированных мясных пород наблюдается в Свердловской области, прирост составил 37 %, общее поголовье начитывает 3056 голов. Поголовье крупного рогатого скота в Курганской области выросло на 28% и составило 9873 головы. В Тюменской области поголовье крупного рогатого скота специализированных мясных пород выросло на 5,8 % и в 2018 году составило 14032 головы. В Челябинской области произошло снижение поголовья специализированных мясных пород крупного рогатого скота на 17,4 %, вместе с тем, субъект остается лидером по количеству голов в Уральском федеральном округе Российской Федерации - 34046 голов. Лидером по численности маточного поголовья специализированного мясного скота является Челябинская область с показателем в 2018 году 15692 голов коров. На втором месте находится Тюменская область с поголовьем мясных коров 6563. Курганская и Свердловская области имеют маточное поголовье 4552 и 1552 соответственно. Основной, а в Свердловской и Челябинской областях порой и единственной, породой является герефордская. Наибольшее количество породного разнообразия мясного скота отмечается в Курганской и Тюменской областях.
The article presents the analysis of the specialized beef cattlebreeding in the Urals Federal District of the Russian Federation: Chelyabinsk Region, Tyumen Region (without autonomous districts), Sverdlovsk Region, Kurgan Region, the structure and species composition of the livestock. Studies are presented for the periods from 2016 to 2018. So, the largest increase in livestock of cattle of specialized meat breeds is observed in the Sverdlovsk region where an increase amounted to 37%, the total number of cattle reached 3056 heads. The number of cattle in the Kurgan region grew by 28% and amounted to 9873 heads. In the Tyumen region, the livestock of cattle specialized meat breeds increased by 5.8%, and in 2018 it amounted to 14032 heads. In the Chelyabinsk Region, the number of specialized beef cattle decreased by 17.4%, while the region remained the leader in the Urals Federal District of the Russian Federation with 34046 heads 0of beef cattle. The leader in the number of breeding stock of specialized beef cattle is the Chelyabinsk region, with 15,692 cows in 2018. The Tyumen region ranks the second with 6563 cows. The Kurgan and Sverdlovsk regions have a breeding stock of 4552 and 1552, respectively. The main, and sometimes the only breed in the Sverdlovsk and Chelyabinsk regions is the Hereford breed. The bigger diversity of beef cattle is noted in the Kurgan and Tyumen regions.
ПЕРСПЕКТИВНЫЕ ОТРАСЛИ ЭКОНОМИЧЕСКОГО РАЗВИТИЯ РЕГИОНА
Authors: Джалалов К.З. , Булатов М.М. 
Published: August 2024

В статье изучены самые перспективные каналы экономики Республики Дагестан. Было подмечено, что государство играет во всех этих направлениях роль компаса. Также в работе изучены проблемы, которые могут оказывать сопротивление развитию сельского хозяйства. Ключевые слова: сельское хозяйство, блокчейн, IT, государство, кластер.
The article examines the most promising channels of the economy of the Republic of Dagestan. It was noticed that the state plays the role of a compass in all these directions. The paper also examines the problems that may resist the development of agriculture.