bluuebunny/crossref_metadata_2025_split huggingface dataset:
Outcome of Pregnancy in Italian Patients with Primary Sjögren Syndrome
Authors: Priori Roberta , Gattamelata Angelica , Modesti Mariagrazia , Colafrancesco Serena , Frisenda Silvia , Minniti Antonina , Framarino-dei-Malatesta Marialuisa , Maset Marta , Quartuccio Luca , De Vita Salvatore , Bartoloni Elena , Alunno Alessia , Gerli Roberto , Strigini Francesca , Baldini Chiara , Tani Chiara , Mosca Marta , Bombardieri Stefano , Valesini Guido 
Published: June 2013

Objective.To investigate pregnancy and fetal outcomes in patients with primary Sjögren syndrome (pSS).Methods.An obstetric history of 36 women with established diagnosis of pSS at pregnancy was obtained from a multicenter cohort of 1075 patients. In a subgroup case-control analysis, 12 deliveries in patients with pSS were compared with 96 control deliveries.Results.Thirty-six women (31 with anti-SSA/Ro and/or anti-SSB/La antibodies) with an established diagnosis of pSS had 45 pregnancies with the delivery of 40 newborns. Two miscarriages, 2 fetal deaths, and 1 induced abortion were recorded. Mean age at the first pregnancy was 33.9 years; mean number of pregnancies was 1.25; 18/40 (45%) cesarean births were delivered; mean pregnancy length was 38.5 weeks (range 32–43), with 6 preterm deliveries. The mean Apgar score at 5 min was 8.9, mean birthweight was 2920 g (range 826–4060 g). Congenital heart block (CHB) occurred in 2/40 (5%) newborns. The reported rate of breastfeeding for at least 1 month was 60.5%. In 4/40 pregnancies (10%) a flare of disease activity was observed within a year from delivery. In the case-control subgroup analysis, 12 deliveries were compared with 96 controls and no significant differences were found.Conclusion.Patients with pSS can have successful pregnancies, which might be followed by a mild relapse. CHB was the only cause of death for offspring of mothers with pSS.
The Healthy Skin Project: Changing Nursing Practice to Prevent and Treat Hospital-Acquired Pressure Ulcers
Authors: Armour-Burton Teri , Fields Willa , Outlaw Lanie , Deleon Elvira 
Published: June 2013

Background
Hospital-acquired pressure ulcers are serious clinical complications that can lead to increased length of stay, pain, infection, and, potentially, death. The surgical progressive care unit at Sharp Grossmont Hospital, San Diego, California, developed the multidisciplinary Healthy Skin Project to decrease the prevalence of hospital-acquired pressure ulcers.

Methods
The previous treatment plan was reviewed and modified according to current evidence-based practice. The project consisted of 3 components: creation of a position for a unit-based wound liaison nurse, staff education, and involvement of the nursing assistants. The wound liaison nurse developed and conducted bimonthly skin audits, which revealed inconsistencies in clinical practice and documentation. Education for the staff was accomplished via a self-learning module, case presentations, and 1-on-1 training. In addition, a pressure ulcer algorithm tool was developed to demonstrate step-by-step wound management and documentation.

Results
From Spring 2003 through Summer 2006, the prevalence of hospital-acquired pressure ulcers ranged from 0.0% to 18.92%, with a mean of 4.85%. After implementation of the project, the prevalence decreased to 0.0% for 17 of 20 quarters, through 2011.

Conclusion
Prevention and a multidisciplinary approach are effective in reducing the occurrence of hospital-acquired pressure ulcers.
Myeloid Hypoxia-Inducible Factor-1α Is Essential for Skeletal Muscle Regeneration in Mice
Authors: Scheerer Nina , Dehne Nathalie , Stockmann Christian , Swoboda Sandra , Baba Hideo A. , Neugebauer Agnes , Johnson Randall S. , Fandrey Joachim 
Published: June 2013

AbstractThe outstanding regeneration ability of skeletal muscle is based on stem cells that become activated and develop to myoblasts after myotrauma. Proliferation and growth of myoblasts result in self-renewal of skeletal muscle. In this article, we show that myotrauma causes a hypoxic microenvironment leading to accumulation of the transcription factor hypoxia-inducible factor-1 (HIF-1) in skeletal muscle cells, as well as invading myeloid cells. To evaluate the impact of HIF-1 in skeletal muscle injury and repair, we examined mice with a conditional HIF-1α knockout targeted to skeletal muscle or myeloid cells in a model of soft tissue trauma. No differences in acute trauma size were detected between control and HIF-1α knockout mice. However, muscles of myeloid HIF-1α knockout mice showed a significant delay in myoblast proliferation and growth of regenerating myofibers, in association with decreased expression of cyclooxygenase-2 in HIF-1α–deficient myeloid cells. Moreover, the removal of necrotic cell debris and the regeneration of endothelial cell structure were impaired in myeloid HIF-1α knockout mice that showed delayed invasion of macrophages to the injury site. Our findings for the first time, to our knowledge, demonstrate that myeloid HIF-1α is required for adequate skeletal muscle regeneration.
Human Plasmacytoid Dendritic Cells Efficiently Capture HIV-1 Envelope Glycoproteins via CD4 for Antigen Presentation
Authors: Sandgren Kerrie J. , Smed-Sörensen Anna , Forsell Mattias N. , Soldemo Martina , Adams William C. , Liang Frank , Perbeck Leif , Koup Richard A. , Wyatt Richard T. , Karlsson Hedestam Gunilla B. , Loré Karin 
Published: June 2013

Abstract
Advances in HIV-1 vaccine clinical trials and preclinical research indicate that the virus envelope glycoproteins (Env) are likely to be an essential component of a prophylactic vaccine. Efficient Ag uptake and presentation by dendritic cells (DCs) is important for strong CD4+ Th cell responses and the development of effective humoral immune responses. In this study, we examined the capacity of distinct primary human DC subsets to internalize and present recombinant Env to CD4+ T cells. Consistent with their specific receptor expression, skin DCs bound and internalized Env via C-type lectin receptors, whereas blood DC subsets, including CD1c+ myeloid DCs, CD123+ plasmacytoid DCs (PDCs), and CD141+ DCs exhibited a restricted repertoire of C-type lectin receptors and relied on CD4 for uptake of Env. Despite a generally poor capacity for Ag uptake compared with myeloid DCs, the high expression of CD4 on PDCs allowed them to bind and internalize Env very efficiently. CD4-mediated uptake delivered Env to EEA1+ endosomes that progressed to Lamp1+ and MHC class II+ lysosomes where internalized Env was degraded rapidly. Finally, all three blood DC subsets were able to internalize an Env-CMV pp65 fusion protein via CD4 and stimulate pp65-specific CD4+ T cells. Thus, in the in vitro systems described in this paper, CD4-mediated uptake of Env is a functional pathway leading to Ag presentation, and this may therefore be a mechanism used by blood DCs, including PDCs, for generating immune responses to Env-based vaccines.
Leishmania-Infected MHC Class IIhigh Dendritic Cells Polarize CD4+ T Cells toward a Nonprotective T-bet+ IFN-γ+ IL-10+ Phenotype
Authors: Resende Mariana , Moreira Diana , Augusto Jorge , Cunha Joana , Neves Bruno , Cruz Maria Teresa , Estaquier Jérôme , Cordeiro-da-Silva Anabela , Silvestre Ricardo 
Published: June 2013

Abstract
A differential behavior among infected and bystander dendritic cells (DCs) has been explored in different infection models. We have analyzed both populations sorted on contact with visceral Leishmania infantum on a susceptible mice model evaluating the subsequent repercussions on adaptive immune response. Our results demonstrate a clear dichotomy between the immunomodulatory abilities of bystander and infected DCs. The bystander population presents increased levels of IL-12p40 and costimulatory molecules being capable to induce CD4+ T cell activation with immune protective capabilities. In contrast, infected DCs, which express lower costimulatory molecules and higher levels of IL-10, promote the development of Leishmania Ag-specific, nonprotective T-bet+IFN-γ+IL-10+ CD4+ T cells with an effector phenotype. This specific polarization was found to be dependent on IL-12p70. Splenic infected DCs recovered from chronic infected animals are similarly capable to polarize ex vivo syngeneic naive CD4+ T cells toward a T-bet+IFN-γ+IL-10+ phenotype. Further analysis revealed that only MHC class IIhigh–infected DCs were responsible for this polarization. The adoptive transfer of such polarized CD4+ T cells facilitates visceral leishmaniasis in BALB/c mice in a clear contrast with their counterpart generated with bystander DCs that significantly potentiate protection. Further, we demonstrated that CD4+ T cells primed by infected DCs in an IL-10 free system, thus deprived of T-bet+IFN-γ+IL-10+ population, restore the immune response and reduce parasite load, supporting a deleterious role of IFN-γ+IL-10+ T cells in the maintenance of infection. Overall, our results highlight novel subversion mechanisms by which nonprotective T-bet+IFN-γ+IL-10+ T cells are associated with chronicity and prolonged parasite persistence.
Inhibition of Histone Deacetylase Activity Suppresses IFN-γ Induction of Tripartite Motif 22 via CHIP-Mediated Proteasomal Degradation of IRF-1
Authors: Gao Bo , Wang Yaxin , Xu Wei , Li Shangshan , Li Qiao , Xiong Sidong 
Published: June 2013

Abstract
Tripartite motif (TRIM)22 plays an important role in IFN-mediated antiviral activity. We previously demonstrated that IFN regulatory factor (IRF)-1 was crucial for basal and IFN-induced TRIM22 transcription via binding to a novel cis-element named 5′ extended IFN-stimulating response element. In this study, we investigated the role of histone deacetylase (HDAC) activity in TRIM22 induction by IFN-γ and its underlying mechanism. We found that the HDAC activity, especially that conferred by HDAC6, was required for IFN-γ–induced TRIM22 transcription. Importantly, inhibition of HDAC activity by trichostatin A (TSA) enhanced the hyperacetylation of heat shock protein (HSP)90 and suppressed its chaperone activity for IRF-1. Further study showed that TSA treatment promoted the proteasomal degradation of IRF-1 protein via enhancing the association of IRF-1 with the ubiquitin E3 ligase carboxyl terminus of Hsc70-interacting protein. Moreover, carboxyl terminus of Hsc70-interacting protein was found to be involved in the TSA-mediated inhibitory effect on IFN-γ induction of TRIM22 as well as other IRF-1–dependent IFN-stimulated genes. This study may provide novel insight into the role of HDAC activity in the transcriptional control of IFN-stimulated gene induction.
Ectopic Expression of Epidermal Antigens Renders the Lung a Target Organ in Paraneoplastic Pemphigus
Authors: Hata Tsuyoshi , Nishimoto Shuhei , Nagao Keisuke , Takahashi Hayato , Yoshida Kazue , Ohyama Manabu , Yamada Taketo , Asano Koichiro , Amagai Masayuki 
Published: June 2013

Abstract
Paraneoplastic pemphigus (PNP) is an autoimmune disease of the skin and mucous membranes that can involve fatal lung complications. IgG autoantibodies target the cell adhesion molecules desmoglein (Dsg)3 and plakins, but the nature and targets of infiltrating T cells are poorly characterized. Moreover, the lung involvement in this skin Ag-specific autoimmune condition represents a paradox. To mimic autoimmunity in PNP, we grafted wild-type skin onto Dsg3−/− mice, which resulted in graft rejection and generation of anti-Dsg3 IgG and Dsg3-specific T cells. Transfer of splenocytes from these mice into Rag2−/− mice induced a combination of suprabasilar acantholysis and interface dermatitis, a histology unique to PNP. Furthermore, the recipient mice showed prominent bronchial inflammation of CD4+ and CD8+ T cells with high mortality. Intriguingly, ectopic Dsg3 expression was observed in the lungs of PNP mice, mirroring the observation that squamous metaplasia is often found in the lungs of PNP patients. Dsg3 and other epidermal Ags were ectopically expressed in the lungs after pulmonary injuries by naphthalene, which was sufficient for recruitment of Dsg3-specific CD4+ T cells. These findings demonstrate that squamous metaplasia after pulmonary epithelial injury may play a crucial role in redirecting the skin-specific autoimmune reaction to the lungs in PNP.
Template‐Assisted Formation of Rattle‐type V2O5 Hollow Microspheres with Enhanced Lithium Storage Properties
Authors: Wu Hao Bin , Pan Anqiang , Hng Huey Hoon , Lou Xiong Wen (David) 
Published: May 2013

AbstractIn this work, rattle‐type ball‐in‐ball V2O5 hollow microspheres are controllably synthesized with the assistance of carbon colloidal spheres as hard templates. Carbon spheres@vanadium‐precursor (CS@V) core–shell composite microspheres are first prepared through a one‐step solvothermal method. The composition of solvent for the solvothermal synthesis has great influence on the morphology and structure of the vanadium‐precursor shells. V2O5 hollow microspheres with various shell architectures can be obtained after removing the carbon microspheres by calcination in air. Moreover, the interior hollow shell can be tailored by varying the temperature ramping rate and calcination temperature. The rattle‐type V2O5 hollow microspheres are evaluated as a cathode material for lithium‐ion batteries, which manifest high specific discharge capacity, good cycling stability and rate capability.
Element free Galerkin modeling of radiative hydromagnetic micropolar flow saturated Darcy medium with heat transfer over a stretching sheet with Joule heating
Authors: Sharma Rajesh 
Published: May 2013

ABSTRACTIn this paper, the effect of thermal radiation on heat transfer in the steady, incompressible hydromagnetic flow of an electrically conducting, micropolar fluid through a Darcian porous medium adjacent to a stretching sheet under a uniform transverse magnetic field has been examined. The partial differential conservation equations governing the flow regime have been nondimensionalized using appropriate similarity transformations and the resulting ordinary differential equations have been solved numerically by using the element‐free Galerkin method. The influence of dimensional less flow parameters on the velocity, microrotation, and temperature distribution have been studied graphically. Excellent correlation of selected results has been achieved with the finite element method. Dimensionless temperatures have been shown to be considerably increased with higher thermal radiation contribution. The flow is also shown to be decelerated in the boundary layer with increasing magnetic field, whereas microrotation of the micro‐elements is amplified with increasing magnetic field and temperatures are also elevated. Increasing porosity of the regime accelerates the flow and also boosts microrotation on the surface of sheet but depresses temperatures in the regime. The mathematical model has important applications in polymeric sheet processing at high temperatures, magnetic materials processing, hydromagnetic control of conducting polymeric sheets, and so on. © 2013 Curtin University of Technology and John Wiley & Sons, Ltd.
Enantioselective Organocatalytic Domino Michael/Aldol Reactions: An Efficient Procedure for the Stereocontrolled Construction of 2H‐Thiopyrano[2,3‐b]quinoline Scaffolds
Authors: Wu Lulu , Wang Youming , Song Haibin , Tang Liangfu , Zhou Zhenghong , Tang Chuchi 
Published: May 2013

AbstractAn efficient procedure for the stereocontrolled construction of 2H‐thiopyrano[2,3‐b]quinoline scaffolds has been developed, starting from simple compounds. The domino Michael/aldol reactions between 2‐mercaptobenzaldehydes and enals, promoted by chiral diphenylprolinol TMS ether, proceed with excellent chemo‐ and enantioselectivity to give the corresponding synthetically useful and pharmaceutically valuable 2H‐thiopyrano[2,3‐b]quinolines in high yields with 90–99 % ee.
Strain‐ and temperature‐dependent changes of fatty acid composition in Wickerhamomyces anomalus and Blastobotrys adeninivorans
Authors: Olstorpe Matilda , Pickova Jana , Kiessling Anders , Passoth Volkmar 
Published: May 2013

AbstractThe fatty acid (FA) profiles of two strains of the yeasts Wickerhamomyces anomalus and Blastobotrys (Arxula) adeninivorans at cultivation temperatures from 15 to 30 °C were characterized. Besides the common even‐numbered C16 and C18 FAs, substantial proportions of the uneven‐numbered C17:1 were found in both species. C18:3(n‐3) (alpha linolenic acid) made up to 3% of the total FAs in all strains. Considerable strain differences occurred, with regard to both the presence of single FAs and parameters like the double binding index (DBI) and C16:C18 ratio. W. anomalus J121 formed C18:1(n‐5) (up to 10.9% of the total FAs) but no C18:1(n‐7), whereas in W. anomalus VKM160, no C18:1(n‐5) was found but up to 14.6% C18:1(n‐7). Similarly, B. adeninivorans CBS 8244 formed exclusively C18:1(n‐7) (maximum 9%) and CBS 7377 C18:1(n‐5) (maximum 12.6%). W. anomalus J121 had the lowest DBI (0.72) at 15 °C and the highest (0.92) at 20 °C, at which point the values decreased with increasing temperatures. In W. anomalus VKM160 and both B. adeninivorans strains, DBI was highest at 15 °C and decreased with increasing temperature. In J121, the C16:C18 ratio was highest at 15 °C, decreasing at higher temperatures, whereas in the other strains, the opposite trend was observed.
On the Decision to Explore New Alternatives: The Coexistence of Under‐ and Over‐exploration
Authors: Teodorescu Kinneret , Erev Ido 
Published: May 2013

ABSTRACTThe decision whether to explore new alternatives or to choose from familiar ones is implicit in many of our daily activities. How is this decision made? When will deviation from optimal exploration be observed? The current paper examines exploration decisions in the context of a multi‐alternative “decisions from experience” task. In each trial, participants could choose a familiar option (the status quo) or a new alternative (risky exploration). The observed exploration rates were more sensitive to the frequent outcome from choosing new alternatives than to the average outcome. That is, the implicit decision whether to explore a new alternative reflects underweighting of rare events: Over‐exploration was documented in “Rare Disasters” environments, and insufficient exploration was evident in “Rare Treasures” environments. In addition, the results reveal a decrease in exploration of new alternatives over time even when it is always optimal and some exploration even when it is never reinforcing. These results can be captured with models that share a distinction between “data collection” and “outcome‐driven” decision modes. Under the data collection mode, the decision maker collects information about the environment, to be used in future choices. Under the outcome‐driven mode, the decision maker relies on small samples of previous experiences with familiar versus unfamiliar alternatives, before the selection of a specific alternative. The predictive value of a two‐parameter “explorative sampler” quantification of these assumptions is demonstrated. Copyright © 2013 John Wiley & Sons, Ltd.
Chemiluminescence determination of moxifloxacin in pharmaceutical and biological samples based on its enhancing effect of the luminol–ferricyanide system using a microfluidic chip
Authors: Suh Yeoun Suk , Kamruzzaman Mohammad , Alam Al‐Mahmnur , Lee Sang Hak , Kim Young Ho , Kim Gyu‐Man , Dang Trung Dung 
Published: May 2013

ABSTRACTA sensitive determination of a synthetic fluoroquinolone antibacterial agent, moxifloxacin (MOX), by an enhanced chemiluminescence (CL) method using a microfluidic chip is described. The microfluidic chip was fabricated by a soft‐lithographic procedure using polydimethyl siloxane (PDMS). The fabricated PDMS microfluidic chip had three‐inlet microchannels for introducing the sample, chemiluminescent reagent and oxidant, and a 500 µm wide, 250 µm deep and 82 mm long microchannel. An enhanced CL system, luminol–ferricyanide, was adopted to analyze the MOX concentration in a sample solution. CL light was emitted continuously after mixing luminol and ferricyanide in the presence of MOX on the PDMS microfluidic chip. The amount of MOX in the luminol–ferricyanide system influenced the intensity of the CL light. The linear range of MOX concentration was 0.14–55.0 ng/mL with a correlation coefficient of 0.9992. The limit of detection (LOD) and limit of quantification (LOQ) were 0.06 and 0.2 ng/mL respectively. The presented method afforded good reproducibility, with a relative standard deviation (RSD) of 1.05% for 10 ng/mL of MOX, and has been successfully applied for the determination of MOX in pharmaceutical and biological samples. Copyright © 2013 John Wiley & Sons, Ltd.
Energy hyperspace for stacking interaction in AU/AU dinucleotide step: Dispersion‐corrected density functional theory study
Authors: Mukherjee Sanchita , Kailasam Senthilkumar , Bansal Manju , Bhattacharyya Dhananjay 
Published: May 2013

ABSTRACTDouble helical structures of DNA and RNA are mostly determined by base pair stacking interactions, which give them the base sequence‐directed features, such as small roll values for the purine–pyrimidine steps. Earlier attempts to characterize stacking interactions were mostly restricted to calculations on fiber diffraction geometries or optimized structure using ab initio calculations lacking variation in geometry to comment on rather unusual large roll values observed in AU/AU base pair step in crystal structures of RNA double helices. We have generated stacking energy hyperspace by modeling geometries with variations along the important degrees of freedom, roll, and slide, which were chosen via statistical analysis as maximally sequence dependent. Corresponding energy contours were constructed by several quantum chemical methods including dispersion corrections. This analysis established the most suitable methods for stacked base pair systems despite the limitation imparted by number of atom in a base pair step to employ very high level of theory. All the methods predict negative roll value and near‐zero slide to be most favorable for the purine–pyrimidine steps, in agreement with Calladine's steric clash based rule. Successive base pairs in RNA are always linked by sugar–phosphate backbone with C3′‐endo sugars and this demands C1′–C1′ distance of about 5.4 Å along the chains. Consideration of an energy penalty term for deviation of C1′–C1′ distance from the mean value, to the recent DFT‐D functionals, specifically ωB97X‐D appears to predict reliable energy contour for AU/AU step. Such distance‐based penalty improves energy contours for the other purine–pyrimidine sequences also. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 107–120, 2014.
Photoinduced Charge‐Transfer Interactions on a Graphene/Block Copolymer Electrostatically Bound to Tetracationic Porphyrin in Aqueous Media
Authors: Skaltsas Theodosis , Pispas Stergios , Tagmatarchis Nikos 
Published: May 2013

AbstractStable dispersions of exfoliated graphene in aqueous media with the aid of the amphiphilic block copolymer poly(isoprene‐b‐acrylic acid) (PI‐b‐PAA), in the form of its anion, were used to electrostatically bind cationic 5,10,15,20‐tetrakis(1‐methyl‐4‐pyridinio)porphine tetra(p‐toluenesulfonate) (H2P4+). A new graphene/PI‐b‐PAA−–H2P4+ ensemble was formed and examined by dynamic light scattering, UV/Vis and fluorescence emission spectroscopy. The efficient fluorescence quenching of H2P4+ in the graphene/PI‐b‐PAA−–H2P4+ ensemble was probed by using steady‐state and time‐resolved photoluminescence, suggesting that electron/energy‐transfer phenomena occur within the nanoensemble. Blank experiments validated the concept of electrostatic interactions that govern the formation of graphene/PI‐b‐PAA−–H2P4+ ensemble, which signified the importance of graphene as an electron acceptor toward the preparation of some new donor–acceptor systems. Finally, kinetic analysis of the lifetime profiles of the fluorescence emission gave information regarding the quenching rate constant and quantum yield of the singlet excited state of H2P4+ in the graphene/PI‐b‐PAA−–H2P4+ ensemble.