bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation on the flexural behavior of high‐strength fiber‐reinforced concrete
Authors: de Almeida Ricardo Laguardia Justen , Parsekian Guilherme Aris , Carnio Marco Antonio 
Published: August 2024

AbstractThe present research investigated the flexural behavior of high‐strength fiber‐reinforced concrete (HSFRC) with six different types of fiber: hooked‐end and crimped steel fibers, chopped and pultruded glass fibers; monofilament and twisted polymeric fibers. An extensive experimental program with 108 specimens for 3‐point bending tests was carried out and the parameters analyzed were the fiber content (0.50%, 0.75% and 1.00% in volume), the fiber type and the compressive strength of the concrete (60 and 90 MPa). Statistical analyses were performed and showed that regardless of the fiber material, increasing the amount of fibers added to concrete increases residual strength and toughness. Limit of proportionality is not affected by the addition of fibers, while postcracking behavior, on the other hand, is controlled by the fibers. Increasing the compressive strength of the concrete matrix affect mainly the limit proportionality of all mixes and the postcracking behavior of the concrete reinforced with hooked‐end steel fibers. The classification of HSFRC and the conditions required for HSFRC for use in structural applications according to the fib Model Code 2010 were also discussed, and it was verified that such conditions are not consistent with the experimental results and should be revised for high‐strength fiber‐reinforced concrete.
Rhodiola and Salidroside Attenuate Oxidative Stress‐Triggered H9c2 Cardiomyoblast Apoptosis Through IGF1R‐Induced ERK1/2 Activation
Authors: Ju I‐Ju , Tsai Bruce Chi‐Kang , Kuo Wei‐Wen , Kuo Chia‐Hua , Lin Yueh‐Min , Hsieh Dennis Jine‐Yuan , Pai Pei‐Ying , Huang Shang‐En , Lu Shang‐Yeh , Lee Shin‐Da , Huang Chih‐Yang 
Published: August 2024

ABSTRACTOxidative stress is a pivotal factor in the pathogenesis of various cardiovascular diseases. Rhodiola, a traditional Chinese medicine, is recognized for its potent antioxidant properties. Salidroside, a phenylpropanoid glycoside derived from Rhodiola rosea, has shown remarkable antioxidant capabilities. This study aimed to elucidate the potential protective mechanisms of Rhodiola and salidroside against H2O2‐induced cardiac apoptosis in H9c2 cardiomyoblast cells. H9c2 cells were exposed to H2O2 for 4 h, and subsequently treated with Rhodiola or salidroside for 24 h. Cell viability and apoptotic pathways were assessed. The involvement of insulin‐like growth factor 1 receptor (IGF1R) and the activation of extracellular regulated protein kinases 1/2 (ERK1/2) were investigated. H2O2 (100 μM) exposure significantly induced cardiac apoptosis in H9c2 cells. However, treatment with Rhodiola (12.5, 25, and 50 μg/mL) and salidroside (0.1, 1, and 10 nM) effectively attenuated H2O2‐induced cytotoxicity and apoptosis. This protective effect was associated with IGF1R‐activated phosphorylation of ERK1/2, leading to the inhibition of Fas‐dependent proteins, HIF‐1α, Bax, and Bak expression in H9c2 cells. The images from hematoxylin and eosin staining and immunofluorescence assays also revealed the protective effects of Rhodiola and salidroside in H9c2 cells against oxidative damage. Our findings suggest that Rhodiola and salidroside possess antioxidative properties that mitigate H2O2‐induced apoptosis in H9c2 cells. The protective mechanisms involve the activation of IGF1R and subsequent phosphorylation of ERK1/2. These results propose Rhodiola and salidroside as potential therapeutic agents for cardiomyocyte cytotoxicity and apoptosis induced by oxidative stress in heart diseases. Future studies may explore their clinical applications in cardiac health.
ERα Coregulator TRIM28 Promotes Breast Cancer Progression by Activating the AKT/GSK3β Pathway
Authors: Fu Linlin , Ma Baodong , Zhang Lan , Xu Huikang , Chen Wei , Wu Di , Gao Feng , Huo Yanping 
Published: August 2024

ABSTRACTEstrogen receptor α (ERα) promotes the growth and survival of ER‐positive breast cancer (BC) cells. ER regulates ER expression target genes by directly binding to specific estrogen response elements, upon activation by estrogens. In this study, 106 proteins interacting with endogenous chromatin‐bound ER in a BC cell line MCF7 were identified using the RIME method. The interactome data showed that the tripartite motif containing 28 (TRIM28) is the most significantly enriched ER‐associated protein. This study provides evidence that TRIM28 expression improves ER transcriptional activity and promotes the BC cells proliferation, migration, and invasion of BC cells. The high expression of TRIM28 is associated with poor clinical outcomes in patients with ER‐positive BC. Mechanistic experiments indicate that TRIM28 expression activates the AKT/GSK3β pathway. To conclude, TRIM28 acts as a regulatory protein of ER and AKT signaling; therefore, it can be a target for the therapeutic interventions of BC.
Impact of dietary arginine supplementation on immune responses and growth performance in Newcastle disease virus‐infected broiler chicks
Authors: Izadi Yazdanabadi Fatemeh , Moghaddam Gholamali , Akbari Mohsen , Abbasabadi Mehdi 
Published: August 2024

AbstractBackgroundNewcastle disease (ND) poses significant challenges within the poultry industry, leading to increased mortality rates, compromised growth, weakened immunity and elevated levels of inflammation.ObjectiveThis study explores the potential of dietary arginine supplementation to ameliorate these adverse effects of ND, leveraging arginine's well‐documented benefits in enhancing growth and immune responses.MethodsA total of 480 one‐day‐old male broiler chicks were meticulously categorised into eight groups, encompassing both infected and noninfected cohorts. These chicks received diets with arginine levels at 85%, 100%, 125% and 150% of recommended standards. The study entailed a comprehensive examination of clinical manifestations, growth performance metrics, haemagglutination inhibition (HI) test results, and serum concentrations of proinflammatory cytokines, adrenocorticotropic hormone (ACTH), and cortisol (CORT).ResultsThe infection significantly curtailed feed consumption (p = 0.0001) and weight gain (p = 0.0001) while concurrently depressing HI titres. Additionally, infected chicks experienced an exacerbated feed conversion ratio (p = 0.0001), escalated mortality rates (p = 0.0001), and elevated serum concentrations of proinflammatory cytokines (p = 0.0001), ACTH (p = 0.0001), and CORT (p = 0.0001). Remarkably, dietary arginine supplementation effectively mitigated the adverse impacts of ND infection on growth, immune responses and proinflammatory cytokine levels. In the context of ND infection, mortality rates and inflammation surge, while growth and immunity are significantly compromised.ConclusionsThe strategic inclusion of arginine in the diet emerges as a potent strategy to counteract the deleterious effects of ND. Supplementation with arginine at levels exceeding the conventional dietary recommendations is recommended to alleviate the detrimental consequences of ND effectively.
Surgeon perspectives on preference cards and environmental stewardship
Authors: Palmisano Zachary M. , Sullivan Gwyneth A. , Petit Hayley J. , Gulack Brian C. , Myers Jonathan , Shah Ami N. 
Published: August 2024

AbstractBackgroundRefinement of surgical preference cards may reduce waste from surgery. This study aimed to characterize surgeon perceptions and practices regarding preference card maintenance, identify barriers to updating preference cards, and explore whether opinions on environmental stewardship relate to preference card maintenance.MethodsThis was a mixed methods survey performed at a single tertiary academic medical center. Surgeons completed questions on accuracy, frequency of updates, and perceived environmental impact of their preference cards. Responses were compared between early career and mid‐to late‐career surgeons using Kruskal–Wallis, chi‐squared, and Fisher's exact tests.ResultsThe response rate was 46.4% (n = 89/192). Among respondents, 46.1% (n = 41/89) rarely or never updated preference cards. Nearly all (98.9%, n = 87/88) said some of their cases had unused items on their cards. Most (87.6%, n = 78/89) made updates via verbal requests. Unfamiliar processes (83.7%, n = 72/86) and effort required (64.0%, n = 55/86) were viewed as barriers to card maintenance. Most agreed that more frequent updates would reduce waste (80.5%, n = 70/87), but respondents did not feel knowledgeable about the environmental impact of items on their cards (62.1%, n = 54/87). Mid‐to late‐career surgeons were less likely to update their cards annually or more often compared to early career surgeons (18.9%, n = 7/37 vs. 57.1%, n = 24/42, p < 0.001). No other responses varied significantly between early career and mid‐to late‐career surgeons.ConclusionsSurgeons acknowledged the utility of preference card maintenance in environmental stewardship, but unfamiliar systems and perceived effort hindered preference card review. Greater attention to preference card maintenance would promote environmentally sustainable practices in surgery.
The caspase-inhibitor Emricasan efficiently counteracts cisplatin- and neomycin-induced cytotoxicity in cochlear cells
Authors: Nassauer Larissa , Schott Juliane W. , Harre Jennifer , Warnecke Athanasia , Morgan Michael , Galla Melanie , Schambach Axel 
Published: August 2024

Abstract
Cisplatin is a chemotherapeutic agent widely used to treat solid tumors. However, it can also be highly ototoxic, resulting in high-frequency hearing loss. Cisplatin causes degeneration of hair cells (HCs) and spiral ganglion neurons (SGNs) in the inner ear, which are essential components of the hearing process and cannot be regenerated in mammals. As the affected cells primarily die by apoptosis, we tested several anti-apoptotic small molecules to protect these cells from drug-induced toxicity. We found that the general caspase inhibitor Emricasan could significantly counteract the toxic effects of cisplatin in House Ear Institute-Organ of Corti 1 (HEI-OC1) cells, phoenix auditory cells, and primary SGNs. Importantly, the anti-cytotoxic effect in neuronal cells was even more pronounced than the effect of sodium thiosulfate (STS), which is currently the only approved prevention option for cisplatin-induced ototoxicity. Finally, we tested the protective effect of Emricasan treatment in the context of another ototoxic drug, i.e., the aminoglycoside antibiotic neomycin, and again found a significant increase in cell viability when the cultures were co-treated with Emricasan. These results suggest a promising strategy to prevent ototoxicity in patients by temporarily blocking the apoptotic pathway when applying cisplatin or aminoglycoside antibiotics.

Key messages

Anti-apoptotic small molecules can reduce cisplatin-induced toxicity.

Emricasan can effectively exert its anti-apoptotic effect on cochlear cells.

Strong protection from cisplatin- and neomycin-induced cytotoxicity with Emricasan.

Sodium thiosulfate and Emricasan provide similar protective effects to cisplatin-treated cells.

Emricasan is more potent than sodium thiosulfate in reducing neomycin-induced cytotoxicity.
Topology optimization for fatigue reserve factors
Authors: Sartorti Roman , Kriegesmann Benedikt , Hawla David , Pedersen Claus B. W. 
Published: August 2024

AbstractThis paper describes a topology optimization approach that applies the common fatigue analysis practices of rainflow cycle counting and critical plane searches to cover both proportional and non-proportional fatigue loading conditions of metals. The existing literature on topology optimization has so far mainly considered fatigue damage under proportional loading conditions and typically uses continuous damage models to avoid the discontinuous nature of fatigue rainflow cycle counting and critical plane searches. Furthermore, previous publications often introduced heuristic schemes to scale the fatigue damage and set the move limits for the design variables rather low to avoid oscillations in the design variables and damage responses during the optimization iterations, because fatigue damage is typically highly localized. Therefore, these approaches cause many optimization iterations. Contrarily, our present approach applies the fatigue reserve factor (FRF) directly in the optimization formulation instead of the fatigue damage where FRF is a fatigue reserve factor for infinite fatigue life. The inverse FRF scales nearly linearly with the stresses. Therefore, the present approach needs no heuristic scaling for the fatigue topology optimization. The numerical implementation applies the semi-analytic adjoint sensitivity method for multiple load cases. Numerically, FRF shows more stable optimization convergence using less optimization iterations. Different FRF topology-optimized designs for a variety of fatigue damage types are validated and compared. Additionally, the optimized FRF designs are compared to both strictly stiffness optimized designs and stress strength optimized designs.
Properties of an optical event timer for satellite laser ranging
Authors: Kodet Jan , Eckl Johann J. , Schreiber K. Ulrich 
Published: August 2024

AbstractThe resolution and above all the stability of the geodetic reference frames is crucially important when global change, such as the sea level rise is observed. In this context systematic errors are still presenting a significant challenge to the measurement techniques of space geodesy. In order to overcome this unfortunate situation for the satellite laser ranging technique, we have utilized the injection of a mode-locked laser to provide a stable low-noise link between the optical domain, where the measurements are carried out, and the microwave regime in which the station clock is defined. We obtained a considerably enhanced measurement delay stability by 10–20 ps over several days, albeit with some experimental challenges. The implementation of waveform scans required us to revisit the issue of target structure and intensity variation in satellite laser ranging.
The role of water in APCI-MS online monitoring of gaseous n-alkanes
Authors: Wentrup Jonas , Dülcks Thomas , Thöming Jorg 
Published: August 2024

AbstractIn atmospheric pressure chemical ionization mass spectrometry (APCI-MS), [M−3H+H2O]+ ions can deliver analyte-specific signals that enable direct analysis of volatile n-alkane mixtures. The underlying ionization mechanisms have been the subject of open debate, and in particular the role of water is insufficiently clarified to allow for reliable process analytics when the humidity level changes over time. This can be a problem, particularly in online monitoring, where analyte accumulation in the ion source can also occur. Here, we investigated the role of water during APCI-MS of volatile n-alkanes by changing the carrier gas for sample injection from a dry to a wetted state as well as by using 18O-labeled water. This allowed for a distinction between gaseous and surface-adsorbed water molecules. While adsorbed water seems to be responsible for the desired [M−3H+H2O]+ signals through surface reactions with the analyte molecules, gaseous water was found to promote the formation of CnH2n+1O+ of different (and analyte-independent) hydrocarbons, revealing a reaction with hydrocarbon species which accumulated in the ion source during continuous operation. At the same time, gaseous water competed with analyte molecules for ionization and thus suppressed the formation of alkyl (CnH2n+1+) and alkenyl (CnH2n−1+) ions. The results reveal a memory effect due to hydrocarbon adsorption, which may cause severe interpretation difficulties when the ionization chamber undergoes sudden humidity changes. The use of [M−3H+H2O]+ for n-alkane analysis in alkane/water mixtures can be facilitated by constantly maintaining high humidity and hence stabilizing the ionization conditions.
Graphical abstract
Assessment of hemodynamic and blood parameters that may reflect macroscopic quality of porcine kidneys during normothermic machine perfusion using whole blood
Authors: Steinhauser Carla , Yakac Abdulbaki Emre , Markgraf Wenke , Kromnik Susanne , Döcke Andreas , Talhofer Philipp , Thiele Christine , Malberg Hagen , Füssel Susanne , Thomas Christian , Putz Juliane 
Published: August 2024

Abstract
Purpose
Using ex vivo normothermic machine perfusion (NMP) with whole blood we assessed marginal porcine kidneys under reperfusion. The aim was to link measureable machine and clinical blood parameters with the currently used visual assessment. This could serve as a baseline for a standardized evaluation score to identify potentially transplantable kidneys in the future.

Methods
Kidneys and autologous whole blood were procured from slaughterhouse pigs (n = 33) and were perfused for 4 h using NMP. The hemodynamic parameters arterial pressure (AP), renal blood flow (RBF) and intrarenal resistance (IRR) were measured. Activity of aspartate transaminase (AST), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and lactate were assessed in blood at 0/1/2/4 h. Kidneys were grouped into “potentially transplantable” (PT) or “not transplantable” (NT) based on their overall macroscopic appearance after NMP by an experienced physician.

Results
PT-kidneys (n = 20) had a significantly lower IRR and higher RBF than NT-kidneys (n = 13). GGT, ALP and LDH did not differ significantly, but at 4 h, AST was significantly higher in PT-kidneys compared to NT-kidneys. Lactate levels kept increasing during NMP in NT-kidneys and were significantly higher at 1/2/4 h than in PT-kidneys.

Conclusion
The immediately assessed macroscopic aspects of examined kidneys correlated with hemodynamic parameters, increased lactate and lower AST in this study. In the future, NMP with whole blood could be a useful tool to extend the donor pool by allowing the assessment of otherwise unknown characteristics of marginal kidneys before transplantation.
Recurrences after nephron-sparing treatments of renal cell carcinoma: a competing risk analysis
Authors: Rosenblad Andreas Karlsson , Hashim Bassam Mazin , Lindblad Per , Ljungberg Börje ,   the National Swedish Kidney Cancer Register Steering Committee
Published: August 2024

Abstract
Purpose
To examine associations between ablative therapy (AT) and partial nephrectomy (PN) and the occurrence of local recurrence (LR), distant metastatic recurrence (DMR) and all-cause mortality in a nation-wide real-world population-based cohort of patients with nonmetastatic renal cell carcinoma (nmRCC).

Methods
Data on 2751 AT- or PN-treated nmRCC tumours diagnosed during 2005–2018, representing 2701 unique patients, were obtained from the National Swedish Kidney Cancer Register. Time to LR/DMR or death with/without LR/DMR was analysed using Cox regression models.

Results
During a mean of 4.8 years follow-up, LR was observed for 111 (4.0%) tumours, DMR for 108 (3.9%) tumours, and death without LR/DMR for 206 (7.5%) tumours. AT-treated tumours had a 4.31 times higher risk of LR (P < 0.001) and a 1.91 times higher risk of DMR (P = 0.018) than PN-treated, with no significant differences in risk of death without LR/DMR. During a mean of 3.2 and 2.5 years of follow-up after LR/DMR, respectively, 24 (21.6%) of the LR cases and 56 (51.9%) of the DMR cases died, compared to 7.5% in patients without LR/DMR. There were no significant differences between AT- and PN-treated regarding risks of early death after occurrence of LR or DMR.

Conclusion
AT treatment of patients with nmRCC implied significantly higher risks of LR and DMR compared with PN treatment. To minimize the risks of LR and DMR, these results suggest that PN is preferred over AT as primary treatment, supporting the EAU guidelines to recommended AT mainly to frail and/or comorbid patients.
Image analysis for bright-field HER2 in situ hybridization: validation for clinical use
Authors: Shi Ruoyu , Pinto João Correia , Rienda Ivan , Caie Peter , Eloy Catarina , Polónia António 
Published: August 2024

AbstractThe aim of the present study was to develop and validate a quantitative image analysis (IA) algorithm to aid pathologists in assessing bright-field HER2 in situ hybridization (ISH) tests in solid cancers. A cohort of 80 sequential cases (40 HER2-negative and 40 HER2-positive) were evaluated for HER2 gene amplification with bright-field ISH. We developed an IA algorithm using the ISH Module from HALO software to automatically quantify HER2 and CEP17 copy numbers per cell as well as the HER2/CEP17 ratio. We observed a high correlation of HER2/CEP17 ratio, an average of HER2 and CEP17 copy number per cell between visual and IA quantification (Pearson’s correlation coefficient of 0.842, 0.916, and 0.765, respectively). IA was able to count from 124 cells to 47,044 cells (median of 5565 cells). The margin of error for the visual quantification of the HER2/CEP17 ratio and of the average of HER2 copy number per cell decreased from a median of 0.23 to 0.02 and from a median of 0.49 to 0.04, respectively, in IA. Curve estimation regression models showed that a minimum of 469 or 953 invasive cancer cells per case is needed to reach an average margin of error below 0.1 for the HER2/CEP17 ratio or for the average of HER2 copy number per cell, respectively. Lastly, on average, a case took 212.1 s to execute the IA, which means that it evaluates about 130 cells/s and requires 6.7 s/mm2. The concordance of the IA software with the visual scoring was 95%, with a sensitivity of 90% and a specificity of 100%. All four discordant cases were able to achieve concordant results after the region of interest adjustment. In conclusion, this validation study underscores the usefulness of IA in HER2 ISH testing, displaying excellent concordance with visual scoring and significantly reducing margins of error.
Early EEG and NIRS measurements in preterm babies: a systematic review
Authors: Llamas-Ramos R. , Alvarado-Omenat J. J. , Llamas-Ramos I. 
Published: August 2024

AbstractPreterm birth represents a public health problem, with prematurity being the leading cause of infant mortality. An objective brain maturation and oxygenation measurement are necessary. The objective has been To test the feasibility of EEG and NIRS combination in the assessment of physiological brain maturation and oxygenation in preterm and non-preterm babies. A systematic review in Pubmed, Web of Science, MEDLINE, Cochrane, Dialnet, CINAHL, Scopus, Lilacs and PEDro databases until December 2022 was developed. 598 registers were found, finally 5 of them reached the inclusion criteria. Two independent reviewers analyzed data and a third reviewer were available for discrepancies. All articles combined EEG and NIRS to assess brain oxygenation and maturation in healthy new-born babies. There is an agreement on the electrode’s placement for EEG at P3 and P4; besides, these regions have been shown to be a development predictive area, as well as the frontoparietal region for the NIRS region and comparison between regions. There is little evidence about the physiological brain electrical activity and oxygenation without stimuli.Conclusion: EEG and NIRS have been useful to assess brain electrical activity and oxygenation in preterm and non-preterm. The combined measurement of these instruments could be essential in neurological disorders diagnosis or their sequels. Unfortunately, the heterogeneity of the results found prevents a consensus on which variables are the most appropriate for the assessment of this population.

What is Known

• Brain assessment could help clinicians to prevent sequels.

• There is an agreement for EEG electrodes placement at P3 and P4 region.

What is New

• EEG and NIRS assessment are effective measurements for preterm babies.

• P3 and P4 regions have shown to be a predictive area of development, as well as the frontoparietal region for NIRS assessment.