bluuebunny/crossref_metadata_2025_split huggingface dataset:
An environmental assessment of the parasite fauna of the reef-associated grouperEpinephelus areolatusfrom Indonesian waters
Authors: Kleinertz S. , Damriyasa I.M. , Hagen W. , Theisen S. , Palm H.W. 
Published: October 2012

AbstractSixtyEpinephelus areolatuswere examined for metazoan fish parasites in Indonesia, off Segara Anakan lagoon, Java and in Balinese waters. The study revealed 21 different parasite species, and 14 new host and locality records. The anisakid nematodesAnisakis typicaand, for the first time in Indonesia,Anisakissp. HC-2005 were identified by using molecular methods. Ecological parameters were calculated for both sites off the anthropogenically influenced Segara Anakan lagoon and the relatively undisturbed reference site at the southern Balinese coast. The fish from Segara Anakan demonstrated a significantly higher enzymatic activity (Hepatosomatic index) and a significantly reduced number of heteroxenous gut helminths (e.g. the digeneanDidymodiclinussp., the nematodeRaphidascarissp. and the acanthocephalanSerrasentis sagittifer). Other regional differences forE. areolatusincluded ecto-/endoparasite ratio, endoparasite diversity, the parasite species composition and prevalence of infection of the respective parasite species. We applied the stargraph method to visualize observed regional differences using grouper parasites as biological indicators for the sampled coastal ecosystems at both sampling sites.
High-risk occupations for suicide
Authors: Roberts S. E. , Jaremin B. , Lloyd K. 
Published: October 2012

BackgroundHigh occupational suicide rates are often linked to easy occupational access to a method of suicide. This study aimed to compare suicide rates across all occupations in Britain, how they have changed over the past 30 years, and how they may vary by occupational socio-economic group.MethodWe used national occupational mortality statistics, census-based occupational populations and death inquiry files (for the years 1979–1980, 1982–1983 and 2001–2005). The main outcome measures were suicide rates per 100 000 population, percentage changes over time in suicide rates, standardized mortality ratios (SMRs) and proportional mortality ratios (PMRs).ResultsSeveral occupations with the highest suicide rates (per 100 000 population) during 1979–1980 and 1982–1983, including veterinarians (ranked first), pharmacists (fourth), dentists (sixth), doctors (tenth) and farmers (thirteenth), have easy occupational access to a method of suicide (pharmaceuticals or guns). By 2001–2005, there had been large significant reductions in suicide rates for each of these occupations, so that none ranked in the top 30 occupations. Occupations with significant increases over time in suicide rates were all manual occupations whereas occupations with suicide rates that decreased were mainly professional or non-manual. Variation in suicide rates that was explained by socio-economic group almost doubled over time from 11.4% in 1979–1980 and 1982–1983 to 20.7% in 2001–2005.ConclusionsSocio-economic forces now seem to be a major determinant of high occupational suicide rates in Britain. As the increases in suicide rates among manual occupations occurred during a period of economic prosperity, carefully targeted suicide prevention initiatives could be beneficial.
Patients with bipolar disorders share similar but attenuated prospective memory impairments with patients with schizophrenia
Authors: Chan R. C. K. , Lui S. S. Y. , Wang Y. , Liu A. C. Y. , Chui W. W. H. , Shum D. H. K. , Cheung E. F. C. 
Published: October 2012

BackgroundProspective memory (PM) refers to the ability to remember to carry out an intended action in the future. PM is consistently found to be impaired in individuals with schizophrenia. Bipolar disorder and schizophrenia may represent conditions along a continuum, and share similar neurocognitive and genetic architecture. This study aimed to compare the nature and extent of PM impairment in individuals with schizophrenia and bipolar disorder.MethodParticipants were 38 out-patients with schizophrenia and 40 out-patients with bipolar disorder in an early psychosis intervention programme, and 37 healthy controls. Time-, event- and activity-based PMs were assessed using a dual-task laboratory paradigm. Self-reported PM performance was gauged using the Prospective and Retrospective Memory Questionnaire. Analysis of covariance (ANCOVA), with intelligence quotient (IQ) and education included as covariates, was used to examine group difference on various types of PM. Repeated measures of ANCOVA were used to examine the group × PM type interaction effect. Correspondence between laboratory and self-reported PM measures was examined using correlational analysis.ResultsThe group × PM type interaction effect was not significant, but the main effect of group was significant. Patients with schizophrenia and patients with bipolar disorder both performed more poorly than healthy participants in PM. The two clinical groups did not significantly differ in PM. Laboratory and self-reported PM measures did not correlate significantly with each other.ConclusionsPatients with bipolar disorder shared a similar PM impairment with those with schizophrenia. Findings of this study extended the similarity in neurocognitive impairments between the two psychiatric disorders to PM.
Endophytic bacterial diversity of an Antarctic moss, Sanionia uncinata
Authors: Park Mira , Lee Hyoungseok , Hong Soon Gyu , Kim Ok-Sun 
Published: October 2012

AbstractAlthough the beneficial effects of endophytic bacteria on their host are significant, the investigation of the microbial diversity in any Antarctic moss has been neglected. In this study, we investigate the endophytic bacterial diversity of the upper green part and the lower brown part of Sanionia uncinata through 16S rRNA genes using pyrosequencing. Proteobacteria was the most dominant phylum with 65.6%, followed by Bacteroidetes (29.1%) and Actinobacteria (11.7%). The different distribution of Alphaproteobacteria between the upper green (2%) and lower brown (22.2%) parts of the moss was significant. Furthermore, dominant and diverse species were detected and closely related to the environmental sequences. These findings suggest that there are likely to be specific relationships between endophytes and host Antarctic moss species.
Nutrient intakes of children aged 1–2 years as a function of milk consumption, cows’ milk or growing-up milk
Authors: Ghisolfi Jacques , Fantino Marc , Turck Dominique , de Courcy Geneviève Potier , Vidailhet Michel 
Published: October 2012

AbstractObjectiveTo evaluate the nutritional adequacy of diets in early childhood as a function of milk intake, cows’ milk (CM) or growing-up milk (GUM).DesignFrom a cross-sectional food consumption survey, two groups of children aged 1–2 years were defined: Group CM fed exclusively on CM ≥ 250 ml/d and Group GUM fed on GUM ≥ 250 ml/d. Proportions of children at risk of nutrient excess or insufficiency were estimated relative to the French Recommended Daily Allowances, Estimated Average Requirements or Adequate Intakes.SettingParents participating in the survey were recruited from all regions of France by a polling organization. Distribution was adjusted to that of the French population.SubjectsSixty-three (Group CM) and fifty-five (Group GUM) children.ResultsTotal energy and macronutrient intakes were similar in the two groups except protein intake of Group CM, which was much higher than the Recommended Daily Allowance and significantly higher than in Group GUM. A high percentage of children of Group CM had intake of linoleic acid (51 %) and α-linolenic acid (84 %) below the lower limit of the Adequate Intake, and intake of Fe (59 %) vitamin C (49 %) and alimentary vitamin D (100 %) less than the Estimated Average Requirement. Significant differences were observed in the proportions of children with a risk of dietary inadequacy between the two groups for all the mentioned nutrients (P < 0·001). In Group GUM, this imbalance was only observed for vitamin D. Intake of foods other than milk and dairy products could not account for these discrepancies.ConclusionsConsumption of CM (≥250 ml/d) entails the risk of insufficiency in α-linolenic acid, Fe, vitamin C and vitamin D. Use of GUM (≥250 ml/d) significantly reduces the risk of insufficiencies in the mentioned nutrients.
Microstructural Characterization of Consolidant Products for Historical Renders: An Innovative Nanostructured Lime Dispersion and a More Traditional Ethyl Silicate Limewater Solution
Authors: Borsoi Giovanni , Tavares Martha , Veiga Rosário , Silva Antonio Santos 
Published: October 2012

AbstractThe conservation and durability of historical renders must be carried out through compatible techniques and materials. An important operation is the restitution of historical renders cohesion, turned friable by the loss of binder, usually due to physical and/or chemical actions. Surface consolidation is based on the use of materials with aggregating properties. This operation is reached usually through the application of organic or mineral consolidants, but inorganic consolidants are becoming preferred due to better compatibility and durability. In this article two mineral compatible consolidation products were studied: a commercial suspension of calcium hydroxide nanoparticles in propanol and a limewater dispersion of ethyl silicate. Microscopy (optical and scanning electron microscopy) and X-ray microanalyses of the consolidation products and of the consolidated mortar specimens were carried out. To assess the mechanical properties and product's efficacy, analyses of the compression, flexural strength, and superficial hardness were performed. Microscopy results show that limewater dispersion of ethyl silicate forms platelike silica gels, which can interfere in product penetration. Otherwise, nanolime particles permit homogeneous distribution and optimum penetration on the treated substrate, improving cementing action and the agglomeration process.
What Remains to Be Done to Allow Quantitative X-Ray Microanalysis Performed with EDS to Become a True Characterization Technique?
Authors: Gauvin Raynald 
Published: October 2012

AbstractThis article reviews different methods used to perform quantitative X-ray microanalysis in the electron microscope and also demonstrates the urgency of measuring the fundamental parameters of X-ray generation for the development of accurate standardless quantitative methods. Using ratios of characteristic lines acquired on the same X-ray spectrum, it is shown that the Cliff and LorimerKA-Bfactor can be used in a general correction method that is appropriate for all types of specimens and electron microscopes, providing that appropriate corrections are made for X-ray absorption, fluorescence, and indirect generation. Since the fundamental parameters appear in theKA-Bfactor, only the ratio of the ionization cross sections needs to be known, not their absolute values. In this regard, the measurement of ratios of theKA-Bfactor (or intensities at different beam energies of the same material with no change of beam spreading in the material) permits the validation for the best models to compute the ratio of ionization cross sections. It is shown, using this method, that the nonrelativistic Bethe equation, to compute ionization cross section, is very close to the equation of E. Casnati et al. (J Phys B15, 155–167, 1982) and also to the equations proposed by D. Bote and F. Salvat (Phys Rev A77, 042701, 2008) for the computation of the ratio of ionization cross sections. The method is extended to show that it could be used to determine the values of the Coster-Kronig transitions factors, an important fundamental parameter for the generation of L and M lines that is mostly known with poor accuracy. The detector efficiency can be measured with specimens where their intensities were measured with an energy dispersive spectrometer detector, the efficiency of which has been measured in an X-ray synchrotron (M. Alvisi et al.,Microsc Microanal12, 406–415, 2006). The spatial resolution should always be computed when performing quantitative X-ray microanalysis and the equations of R. Gauvin (Microsc Microanal13(5), 354–357, 2007) for bulk materials and the one presented in this article for thin films should be used. The effects of X-rays generated by fast secondary electrons and by Auger electrons are reviewed, and their effect can be detrimental for the spatial resolution of materials involving low-energy X-ray lines, in certain specific conditions. Finally, quantitative X-ray microanalysis of heterogeneous materials is briefly reviewed.
Synthetic radiance simulation and evaluation for a Joint Observing System Simulation Experiment
Authors: Zhu Tong , Weng Fuzhong , Masutani Michiko , Woollen Jack S. 
Published: October 2012

Since 2006, a Joint Observing System Simulation Experiment (OSSE) project has been established at the United States Joint Center for Satellite Data Assimilation. The work provided here documents the simulation of synthetic satellite observations from the OSSE Nature Run (NR) and the evaluation of the simulated results. The Community Radiative Transfer Model was used to produce synthetic satellite observations, which will be assimilated in the Gridpoint Statistical Interpolation system. Synthetic radiances were evaluated through a comparison with real observations and model simulations obtained from National Center for Environmental Prediction (NCEP) Global Forecast System (GFS) 6 h forecast fields. For both IR and microwave sensors, we determined that the bias and the standard deviation of the synthetic radiances were in good agreement with real observations. At the NR initial time, the simulated Advanced Microwave Sounding Unit‐A (AMSU‐A) radiances reproduced observed AMSU‐A interchannel correlations and symmetric angular‐dependent features. However, the asymmetric angular‐dependent bias, mainly related to AMSU‐A instrument polarization misalignment, cloud not be simulated properly. The simulated GOES‐12 Sounder radiances indicated that the error characteristics of atmospheric temperature sounding channels were similar to those from the NCEP operational GFS analysis system, and those biases of moisture and surface channels were approximately 2 K. To mimic real observational errors, random (Gaussian distribution) errors with the mean and variance estimated by the differences between observations and GFS fields were added to the synthetic radiances, resulting in a 20–30% increase in the standard deviations of the synthetic radiances.
Structure and function of the PorB porin from disseminating Neisseria gonorrhoeae
Authors: Zeth Kornelius , Kozjak-Pavlovic Vera , Faulstich Michaela , Fraunholz Martin , Hurwitz Robert , Kepp Oliver , Rudel Thomas 
Published: October 2012

The outer membrane of Gram-negative bacteria contains a large number of channel-forming proteins, porins, for the uptake of small nutrient molecules. Neisseria gonorrhoeae PorBIA (PorB of serotype A) are associated with disseminating diseases and mediate a rapid bacterial invasion into host cells in a phosphate-sensitive manner. To gain insights into this structure–function relationship we analysed PorBIA by X-ray crystallography in the presence of phosphate and ATP. The structure of PorBIA in the complex solved at a resolution of 3.3 Å (1 Å=0.1 nm) displays a surplus of positive charges inside the channel. ATP ligand-binding in the channel is co-ordinated by the positively charged residues of the channel interior. These residues ligate the aromatic, sugar and pyrophosphate moieties of the ligand. Two phosphate ions were observed in the structure, one of which clamped by two arginine residues (Arg92 and Arg124) localized at the extraplasmic channel exit. A short β-bulge in β2-strand together with the long L3 loop narrow the barrel diameter significantly and further support substrate specificity through hydrogen bond interactions. Interestingly the structure also comprised a small peptide as a remnant of a periplasmic protein which physically links porin molecules to the peptidoglycan network. To test the importance of Arg92 on bacterial invasion the residue was mutated. In vivo assays of bacteria carrying a R92S mutation confirmed the importance of this residue for host-cell invasion. Furthermore systematic sequence and structure comparisons of PorBIA from Neisseriaceae indicated Arg92 to be unique in disseminating N. gonorrhoeae thereby possibly distinguishing invasion-promoting porins from other neisserial porins.
Temperature‐Related Changes in Habitat Quality and Use by Bonneville Cutthroat Trout in Regulated and Unregulated River Segments
Authors: Hillyard Ryan W. , Keeley Ernest R. 
Published: October 2012

AbstractThe availability of suitable habitat is often viewed as one of most important limiting factors for animal populations. In this study, we examined the composition and spatial distribution of stream habitat based on summer water temperature using airborne thermal imagery, floating temperature surveys, and fixed temperature data loggers in a regulated and unregulated segment of the Bear River in Idaho and Wyoming. We also used temperature‐sensitive radio telemetry tags to measure water temperature in habitats used by Bonneville cutthroat trout Oncorhynchus clarkii utah. We found that when available water temperatures increased in the Bear River, cutthroat trout in the regulated segment continuously selected cooler habitats that followed a similar rate of increase as available water temperatures. In the unregulated segment, cutthroat trout did not select significantly cooler available water temperatures when measured over the entire study period. However, cutthroat trout in the unregulated segment did select cooler water temperatures during the peak period of high water temperatures in July. In the regulated river segment, patches of habitat with hospitable water temperatures were small, infrequent, and widely distributed. Whereas, in the unregulated river segment, habitat patches with hospitable water temperatures were larger, more frequent, and in closer proximity to one another. Our data indicate that during the peak of summer temperatures the availability of habitat below the thermal maxima of cutthroat trout becomes very limited, but they are able to locate and use these small patches of habitat during the warmest part of the summer.
Choosing Here and Now versus There and Later: The Moderating Role of Psychological Distance on Assortment Size Preferences
Authors: Goodman Joseph K. , Malkoc Selin A. 
Published: October 2012

Abstract
Consumers prefer larger assortments, despite the negative consequences associated with choosing from these sets. This article examines the role of psychological distance (temporal and geographical) in consumers’ assortment size decisions and rectifies contradicting hypotheses produced by construal level theory. Six studies demonstrate that while consumers prefer larger assortments when the choice takes place in the here and now, they are more likely to prefer small assortments when choices pertain to distant locations and times. This decrease in preference for large assortments is due to psychological distance increasing the similarity of the options in a category, making them appear more substitutable. This effect of psychological distance reverses when consumers consider desirability/feasibility trade-off information inherent in the assortment size decision. These findings point to important outcomes of psychological distance, resolving opposing predictions of construal level theory, and identify boundary conditions for the well-established notion that consumers are attracted to large assortments.
Implications of M Bias in Epidemiologic Studies: A Simulation Study
Authors: Liu Wei , Brookhart M. Alan , Schneeweiss Sebastian , Mi Xiaojuan , Setoguchi Soko 
Published: October 2012

Abstract
Collider-stratification bias arises from conditioning on a variable (collider) which opens a path from exposure to outcome. M bias occurs when the collider-stratification bias is transmitted through ancestors of exposure and outcome. Previous theoretical work, but not empirical data, has demonstrated that M bias is smaller than confounding bias. The authors simulated data for large cohort studies with binary exposure, an outcome, a collider, and 2 predictors of the collider. They created 178 scenarios by changing the frequencies of variables and/or the magnitudes of associations among the variables. They calculated the effect estimate, percentage bias, and mean squared error. M bias in these realistic scenarios ranged from −2% to −5%. When the authors increased one or both relative risks for the relation between the collider and unmeasured factors to ≥8, the negative bias was more substantial (&gt;15%). The result was substantially biased (e.g., &gt;20%) if an unmeasured confounder that was also a collider was not adjusted to avoid M bias. In scenarios resembling those the authors examined, M bias had a small impact unless associations between the collider and unmeasured confounders were very large (relative risk &gt; 8). When a collider is itself an important confounder, controlling for confounding would take precedence over avoiding M bias.
Sildenafil in Heart Failure (SilHF). An investigator‐initiated multinational randomized controlled clinical trial: rationale and design
Authors: Cooper Trond J. , Guazzi Marco , Al‐Mohammad Abdallah , Amir Offer , Bengal Tuvia , Cleland John G. , Dickstein Kenneth 
Published: October 2012

AimsHeart failure (HF) is a major clinical problem and, despite advances in both pharmacological and device therapy, the mortality remains high and quality of life poor. Over the last decade there has been growing interest in using phosphodiesterase‐5 (PDE‐5) inhibitors in HF associated with group 2 pulmonary hypertension (PH), with benefits reported on pulmonary haemodynamic and functional status in single‐centre trialsMethodsThe Sildenafil in Heart Failure (SilHF) trial is a randomized, placebo‐controlled multinational trial designed to assess efficacy and tolerability of PDE‐5 inhibition with sildenafil (target dose 40 mg three times per day) in 210 patients with HF, New York Heart Association (NYHA) functional class II or III, and evidence of group 2 PH. The co‐primary endpoints are patient global assessment and the 6 min walk test. Secondary endpoints include NYHA functional class and the quality of life tools Euro QoL 5D and the Kansas City questionnaire. Patients will be followed up for 6 months.PerspectiveThe authors hypothesize that PDE‐5 inhibition can improve exercise capacity and symptoms with acceptable tolerability in patients with HF and group 2 PH