bluuebunny/crossref_metadata_2025_split huggingface dataset:
A phase II trial of a multi‐agent oral antiangiogenic (metronomic) regimen in children with recurrent or progressive cancer
Authors: Robison Nathan J. , Campigotto Federico , Chi Susan N. , Manley Peter E. , Turner Christopher D. , Zimmerman Mary Ann , Chordas Christine A. , Werger Annette M. , Allen Jeffrey C. , Goldman Stewart , Rubin Joshua B. , Isakoff Michael S. , Pan Wilbur J. , Khatib Ziad A. , Comito Melanie A. , Bendel Anne E. , Pietrantonio Jay B. , Kondrat Laura , Hubbs Shannon M. , Neuberg Donna S. , Kieran Mark W. 
Published: October 2013

AbstractBackgroundPreclinical models show that an antiangiogenic regimen at low‐dose daily (metronomic) dosing may be effective against chemotherapy‐resistant tumors. We undertook a prospective, open‐label, single‐arm, multi‐institutional phase II study to evaluate the efficacy of a “5‐drug” oral regimen in children with recurrent or progressive cancer.ProcedurePatients ≤21 years old with recurrent or progressive tumors were eligible. Treatment consisted of continuous oral celecoxib, thalidomide, and fenofibrate, with alternating 21‐day cycles of low‐dose cyclophosphamide and etoposide. Primary endpoint was to assess, within eight disease strata, activity of the 5‐drug regimen over 27 weeks. Blood and urine angiogenesis markers were assessed.ResultsOne hundred one patients were enrolled; 97 began treatment. Median age was 10 years (range: 191 days–21 years); 47 (49%) were female. Disease strata included high‐grade glioma (HGG, 21 patients), ependymoma (19), low‐grade glioma (LGG, 12), bone tumors (12), medulloblastoma/primitive neuroectodermal tumor (PNET, 8), leukemia (4), neuroblastoma (3), and miscellaneous tumors (18). Treatment was generally well tolerated; most common toxicities were hematologic. Twenty‐four (25%) patients completed 27 weeks therapy without progression, including HGG: 1 (5%), ependymoma: 7 (37%), LGG: 7 (58%), medulloblastoma/PNET: 1, neuroblastoma: 1, and miscellaneous tumors: 7 (39%). Best response was complete response (one patient with medulloblastoma), partial response (12), stable disease (36), progressive disease (47), and inevaluable (1). Baseline serum thrombospondin levels were significantly higher in patients successfully completing therapy than in those who progressed (P = 0.009).ConclusionThe 5‐drug regimen was well tolerated. Clinical activity was demonstrated in some but not all tumor strata. Pediatric Blood Cancer 2014;61:636–642. © 2013 The Authors Pediatric Blood & Cancer Published by Wiley Periodicals, Inc.
Quality by Design for Herbal Drugs: a Feedforward Control Strategy and an Approach to Define the Acceptable Ranges of Critical Quality Attributes
Authors: Yan Binjun , Li Yao , Guo Zhengtai , Qu Haibin 
Published: October 2013

ABSTRACTIntroductionThe concept of quality by design (QbD) has been widely accepted and applied in the pharmaceutical manufacturing industry. There are still two key issues to be addressed in the implementation of QbD for herbal drugs. The first issue is the quality variation of herbal raw materials and the second issue is the difficulty in defining the acceptable ranges of critical quality attributes (CQAs).ObjectiveTo propose a feedforward control strategy and a method for defining the acceptable ranges of CQAs for the two issues.MethodsIn the case study of the ethanol precipitation process of Danshen (Radix Salvia miltiorrhiza) injection, regression models linking input material attributes and process parameters to CQAs were built first and an optimisation model for calculating the best process parameters according to the input materials was established. Then, the feasible material space was defined and the acceptable ranges of CQAs for the previous process were determined.ResultsIn the case study, satisfactory regression models were built with cross‐validated regression coefficients (Q2) all above 91 %. The feedforward control strategy was applied successfully to compensate the quality variation of the input materials, which was able to control the CQAs in the 90–110 % ranges of the desired values. In addition, the feasible material space for the ethanol precipitation process was built successfully, which showed the acceptable ranges of the CQAs for the concentration process.ConclusionThe proposed methodology can help to promote the implementation of QbD for herbal drugs. Copyright © 2013 John Wiley & Sons, Ltd.
HPLC Profiling with At‐line Microdilution Assay for the Early Identification of Anti‐fungal Compounds in Plants from French Polynesia
Authors: Bertrand Samuel , Petit Charlotte , Marcourt Laurence , Ho Raimana , Gindro Katia , Monod Michel , Wolfender Jean‐Luc 
Published: October 2013

ABSTRACTIntroductionThe search for anti‐fungal compounds has maintained a scientific interest notably due to existing difficulties in the treatment of mycoses and their increasing occurrence in hospitals.ObjectiveDevelopment of a simple method to rapidly identify anti‐fungal compounds in crude plant extracts based on a HPLC microfractionation approach combined with an at‐line anti‐Candida assay.MethodsThe scale of the semi‐preparative HPLC microfractionation was adapted to fit the sensitivity of the Candida albicans anti‐fungal in a 96‐well microdilution assay. This format is also compatible for MS and NMR dereplication of the active compounds.ResultsBased on the screening of 12 crude extracts of plants from French Polynesia, three plants, which displayed various levels of anti‐fungal activities, were selected to assess the efficiency of the HPLC anti‐fungal profiling and the scale necessary for microfractionation. The same anti‐Candida assay was performed on the HPLC microfractions collected using a generic profiling method. Analysis of active microfractions by MS and NMR issued from the most active extract enabled an efficient dereplication of the compounds responsible for the anti‐fungal activity.ConclusionA generic HPLC anti‐fungal profiling method was developed which revealed that only 50 mg of crude extract were sufficient for a rapid identification of compound(s) responsible for the anti‐Candida activity. This approach was illustrated by the study of Alphitonia zizyphoides, a plant traditionally used to treat dermatomycoses. Copyright © 2013 John Wiley & Sons, Ltd.
Laser Microdissection: a Sample Preparation Technique for Plant Micrometabolic Profiling
Authors: Fang Jingjing , Schneider Bernd 
Published: October 2013

ABSTRACTIntroductionUnlike unicellular organisms, plants have evolved as complex organisms that are defined by their ability to distribute special vital functions to spatially separated organs and tissues. Current phytochemical approaches mostly ignore this fact by analysing samples that consist of different cell types and thus average the information obtained. A comprehensive metabolite analysis with high spatial resolution is essential to fully characterise the state of a certain tissue; hence, the analysis of metabolites occurring in specialised plant cells is of considerable interest in chemical ecology, plant natural product chemistry and other bioscience disciplines. Laser microdissection (LMD), including laser capture microdissection and laser microdissection and pressure catapulting, is a convenient sampling technique to harvest homogeneous cell types for the microanalysis of plant metabolites.ObjectiveThe objective of this work is to provide an introduction to LMD methodology and a concise review of recent applications of LMD in the high‐resolution analysis of metabolites in different plant materials.MethodsA step‐by‐step approach to LMD sampling techniques is described. How LMD can be used to sample cells or microscopic tissue pieces from different plant organs, such as leaves, stems, and seeds, is shown in detail. Finally, the future of LMD in plant metabolites analysis is discussed.ResultsThis review summarises studies over the past decade not only showing technical details but also indicating the wide application of this method for high‐resolution plant metabolite analysis.ConclusionLaser microdissection is a powerful sampling technique for plant micrometabolic profiling and metabolomics research. Copyright © 2013 John Wiley & Sons, Ltd.
Identification of dosage‐sensitive genes in fetuses referred with severe isolated congenital diaphragmatic hernia
Authors: Brady P. D. , DeKoninck P. , Fryns J. P. , Devriendt K. , Deprest J. A. , Vermeesch J. R. 
Published: October 2013

ABSTRACTObjectiveCongenital diaphragmatic hernia (CDH) is a fetal abnormality affecting diaphragm and lung development with a high mortality rate despite advances in fetal and neonatal therapy. CDH may occur either as an isolated defect or in syndromic form for which the prognosis is worse. Although conventional karyotyping and, more recently, chromosomal microarrays support a substantial role for genetic factors, causal genes responsible for isolated CDH remain elusive. We propose that chromosomal microarray analysis will identify copy number variations (CNVs) associated with isolated CDH.MethodsWe perform a prospective genome‐wide screen for CNVs using chromosomal microarrays on 75 fetuses referred with apparently isolated CDH, six of which were later reclassified as non‐isolated CDH.ResultsThe results pinpoint haploinsufficiency of NR2F2 as a cause of CDH and cardiovascular malformations. In addition, the 15q25.2 and 16p11.2 recurrent microdeletions are associated with isolated CDH. By using gene prioritisation and network analysis, we provide strong evidence for several novel dosage‐sensitive candidate genes associated with CDH.ConclusionsChromosomal microarray analysis detects submicroscopic CNVs associated with isolated CDH or CDH with cardiovascular malformations. © 2013 John Wiley & Sons, Ltd.
Psychosocial factors affecting uptake of prenatal genetic testing: a pilot study
Authors: Pivetti Monica , Melotti Giannino , Morselli Davide , Olivieri Mariangela 
Published: October 2013

ABSTRACTObjectiveThis study aims to explore the psychosocial factors which influenced the undergoing of prenatal genetic testing (PGT) in a sample of pregnant women.MethodAn online questionnaire was emailed to a convenient sample of 105 pregnant women, recruited from among those attending two antenatal education programmes and via snowball sampling.ResultsA minority of respondents underwent chorionic villous sampling and amniocentesis (12 and 11, respectively). Advanced maternal age was related to undertaking PGT. The path analysis model (χ2 (16, N = 97) = 19.01, ns; TLI = 0.95; RMSEA = 0.04; WRMR = 0.64) confirmed that three dimensions, namely having a more supporting attitude towards PGT (β = 0.54, p < 0.001), perceiving PGT as reliable (β = 0.29, p < 0.05) and the request for more scientific information (β = 0.22, p < 0.01), related to test uptaking.ConclusionEmpirical researches investigating pregnant women's beliefs and attitudes relating to actual test uptake could help genetic counsellors and policy makers to better understand their client's profile in relation to the determining factors affecting the undertaking of PGT. © 2013 John Wiley & Sons, Ltd.
Using aluminium triflate as a co‐catalyst for the polymerization of o‐phenylenediamine and its derivatives
Authors: Amer Ismael , Young Desmond A , Vosloo Hermanus CM 
Published: October 2013

AbstractAromatic diamine monomers, including o‐phenylenediamine (oPD), 4‐methyl‐o‐phenylenediamine (4Me‐oPD), 4,5‐dimethyl‐o‐phenylenediamine (dMe‐oPD) and 4‐(tert‐butyl)‐o‐phenylenediamine (tBu‐oPD), were polymerized by chemical oxidation using ammonium persulfate as an oxidant. Aluminium triflate (Al(OTf)3) was also used for the first time as a co‐catalyst under various reaction conditions for the polymerization of oPD derivatives. The polymerization yield was improved when Al(OTf)3 was introduced to the polymerization reaction for most polymers. The solubility of poly(4‐methyl‐o‐phenylenediamine) (P(4Me‐oPD)), poly(4,5‐dimethyl‐o‐phenylenediamine) (P(dMe‐oPD)) and poly(4‐(tert‐butyl)‐o‐phenylenediamine) (P(tBu‐oPD)) polymers was improved compared with the poly(o‐phenylenediamine) (P(oPD)) polymers in most common solvents. The homopolymers obtained were characterized by Fourier transform IR spectroscopy, UV−visible spectroscopy, 1H and 13C NMR, wide‐angle X‐ray diffraction, DSC and TGA. The results showed that the yield, solubility and structure of the polymers are significantly dependent on the polymerization conditions. DSC measurements indicated that the polymers exhibited melting and crystallization transitions. The polymers also showed good thermal stability and decompose above 400 °C in nitrogen. © 2013 Society of Chemical Industry
UV filter 2‐ethylhexyl 4‐methoxycinnamate: a structure, energetic and UV–vis spectral analysis based on density functional theory
Authors: Miranda Margarida S. , Pinto da Silva Luís , Esteves da Silva Joaquim C. G. 
Published: October 2013

2‐Ethylhexyl 4‐methoxycinnamate (EHMC) is a very commonly used UVB filter that is known to isomerize from the (E) to the (Z) isomer in the presence of light.In this study, we have performed high level quantum chemical calculations using density functional theory (DFT) with the B3LYP density functional and extended basis sets to study the gas‐phase molecular structure of EHMC and its energetic stability. Calculations were also performed for related smaller molecules cinnamic acid and 4‐methoxycinnamic acid.Charge delocalization has been analyzed using natural charges and Wiberg bond indexes within the natural bond orbital analysis and using nucleus independent chemical shifts.Density functional theory calculations reveal that the (E) isomer of EHMC is more stable than the (Z) by about 20 kJ mol−1 in both the gas and aqueous phases. The enthalpy of formation in the gas phase of (E)‐EHMC was derived from an isodesmic bond separation reaction.Long‐range corrected DFT calculations in implicit water were made in order to understand the excited state properties of the (E) and (Z) isomers of EHMC. Copyright © 2013 John Wiley & Sons, Ltd.
Donor–acceptor copolymers based on phenanthrene as electron‐donating unit: Synthesis and photovoltaic performances
Authors: Chen Zhenhui , Cai Ping , Zhang Lianjie , Zhu Yongxiang , Xu Xiaofeng , Sun Jiangman , Huang Jun , Liu Xuncheng , Chen Junwu , Chen Hongzheng , Cao Yong 
Published: October 2013

ABSTRACTUsing 9,10‐bis(dodecyloxy)phenanthrene as electron‐donating unit and 4,7‐dithienyl‐5,6‐bis(dodecyloxy)benzothiadiazole, 4,7‐dithienyl‐5,6‐bis(octyloxy)benzoxadiazole, 5,8‐dithienyl‐2,3‐bis(para‐octyloxyphenyl)quinoxaline, and 5,8‐dithienyl‐2,3‐bis(meta‐octyloxyphenyl)quinoxaline as electron‐accepting unit, four D–A copolymers PPA‐DTBT, PPA‐DTBX, PPA‐p‐DTQ, and PPA‐m‐DTQ, respectively, were successfully synthesized as new polymeric donors for photovoltaic cells. All the alternating copolymers can show two absorption bands, both in solutions and thin films. The optical bandgaps of the polymers are quite close, which are between 1.93 and 2.00 eV. The HOMO and LUMO levels of the polymers are also comparable of −5.52 ± 0.03 eV and −3.57 ± 0.03 eV, respectively. Thus, using the dialkoxyphenanthrene as the D unit could afford D–A copolymers with deep‐lying HOMO levels, which would be an important factor to achieve high open‐circuit voltages (Voc) in bulk‐heterojunction solar cells. With the copolymers as the donor and PC71BM as the acceptor, the resulting solar cells could display good Voc between 0.86 and 0.88 V. Among the four copolymers, PPA‐DTBT containing the dialkoxybenzothiadiazole unit showed the best power conversion efficiency of 3.03% because of its relatively higher hole mobility and better phase separation. The results suggest that dialkoxyphenanthrene is a valuable electron‐donating unit in the constructions of D–A copolymers for efficient solar cells with high Voc. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 4966–4974
Fabrication and characterization of SiO2/PVDF composite nanofiber‐coated PP nonwoven separators for lithium‐ion batteries
Authors: Yanilmaz Meltem , Chen Chen , Zhang Xiangwu 
Published: October 2013

ABSTRACTSiO2/polyvinylidene fluoride (PVDF) composite nanofiber‐coated polypropylene (PP) nonwoven membranes were prepared by electrospinning of SiO2/PVDF dispersions onto both sides of PP nonwovens. The goal of this study was to combine the good mechanical strength of PP nonwoven with the excellent electrochemical properties of SiO2/PVDF composite nanofibers to obtain a new high‐performance separator. It was found that the addition of SiO2 nanoparticles played an important role in improving the overall performance of these nanofiber‐coated nonwoven membranes. Among the membranes with various SiO2 contents, 15% SiO2/PVDF composite nanofiber‐coated PP nonwoven membranes provided the highest ionic conductivity of 2.6 × 10−3 S cm−1 after being immersed in a liquid electrolyte, 1 mol L−1 lithium hexafluorophosphate in ethylene carbonate, dimethyl carbonate and diethyl carbonate. Compared with pure PVDF nanofiber‐coated PP nonwoven membranes, SiO2/PVDF composite fiber‐coated PP nonwoven membranes had greater liquid electrolyte uptake, higher electrochemical oxidation limit, and lower interfacial resistance with lithium. SiO2/PVDF composite fiber‐coated PP nonwoven membrane separators were assembled into lithium/lithium iron phosphate cells and demonstrated high cell capacities and good cycling performance at room temperature. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2013, 51, 1719–1726
Morphological investigation of polydisperse asymmetric block copolymer systems of poly(styrene) and poly(methacrylic acid) in the strong segregation regime
Authors: Asad Ayoubi Mehran , Zhu Kaizheng , Nyström Bo , Olsson Ulf , Almdal Kristoffer , Khokhlov Alexei R. , Piculell Lennart 
Published: October 2013

ABSTRACTSamples of compositionally (highly) asymmetric diblock copolymers and, also, mixtures of diblock and triblock copolymers (the latter obtained as end‐coupling products of two diblock molecules of the mixture), composed of (a) monodisperse majority block(s) of poly(styrene) (PS) and a polydisperse minority block of poly(methacrylic acid) (PMAA), microphase separate into spherical PMAA microdomains, either in disordered liquid‐like state or body‐centered‐cubic (BCC) arrangement, at various annealing temperaturesT, in the strong segregation regime SSR. We found that (i) the microphase separated state is favored over an anticipated molecularly homogenous state, (ii) the spherical microdomain morphology (with BCC symmetry) is favored over an anticipated hexagonally packed cylindrical morphology, (iii) the extent of the dissolution of short PMAA blocks in the PS material can be quantified, (iv) the spherical microdomains are dilated, and (v) despite molecular‐weight (and architectural) polydispersity, well‐ordered BCC structures can be obtained. © 2013 Wiley Periodicals, Inc. J. Polym. Sci. Part B: Polym. Phys.2013,51, 1657–1671
How do researchers conceive of spousal grief after cancer? A systematic review of models used by researchers to study spousal grief in the cancer context
Authors: Fasse Léonor , Sultan Serge , Flahault Cécile , MacKinnon Christopher J. , Dolbeault Sylvie , Brédart Anne 
Published: October 2013

AbstractBackgroundAlthough spouses bereaved after cancer are considered vulnerable people, there have been few empirical studies to explore grief specifically in this context.MethodsUsing PsycINFO, Medline, and the PRISMA statement, we systematically searched the literature by intersecting ‘cancer’ and ‘grie*’, ‘cancer’ and ‘bereave*’, and ‘cancer’ and ‘mourn*’.ResultsGathering 76 studies (2000–2013) that met the inclusion criteria for bereavement in adulthood, bereavement of an adult loved one and evidence‐based research, we found the following:

Spousal relationships are not systematically examined in the current dominant models of grief.
Theoretically derived determinants of spousal grief after cancer and empirically derived ones converge toward the necessity to include the caregiving experience as determining grief reactions.
A growing body of literature concerning prolonged grief disorders now provides integrative reflections regarding the characteristics of spousal loss, predictors, and associated therapeutic interventions in the cancer context.ConclusionsFew empirical studies (20 of 76) target spousal bereavement specifically after cancer. The process of adaptation to loss is usually decontextualized, removing any consideration of the relationship to the deceased or the experience of caregiving and dying. Our findings suggest that this topic warrants more studies that use both prospective and mixed methodologies, as well as explore typical grief needs and experiences of bereaved spouses. Copyright © 2013 John Wiley & Sons, Ltd.
A survey of barriers to screening for oral cancer among rural Black Americans
Authors: Shepperd James A. , Howell Jennifer L. , Logan Henrietta 
Published: October 2013

AbstractObjectiveResearch documents a disparity between Black and White Americans in mortality for oral cancer that appears to result in part from behaviors such as lower oral cancer screening among Black Americans. We examined barriers to oral cancer screening among Black Americans.MethodsWe surveyed Black Americans (N = 366) living in rural Florida to identify barriers to getting screened for oral cancer.ResultsLow knowledge/social attention, lack of resources, and fear/defensive avoidance predicted screening intentions, with lack of resources emerging as the largest barrier. Participants also reported that a recommendation from their provider was most likely to increase screening intentions, whereas encountering financial barriers was most likely to decrease screening intentions.ConclusionsLow knowledge/social attention, lack of resources, and fear/defensive avoidance emerged as independent barriers to oral cancer screening, with the latter two barriers accounting for the most variance in intentions to get screened. Copyright © 2013 John Wiley & Sons, Ltd.
Optimism, social support, and mental health outcomes in patients with advanced cancer
Authors: Applebaum Allison J. , Stein Emma M. , Lord‐Bessen Jennifer , Pessin Hayley , Rosenfeld Barry , Breitbart William 
Published: October 2013

AbstractObjectiveOptimism and social support serve as protective factors against distress in medically ill patients. Very few studies have specifically explored the ways in which these variables interact to impact quality of life (QOL), particularly among patients with advanced cancer. The present study examined the role of optimism as a moderator of the relationship between social support and anxiety, depression, hopelessness, and QOL among patients with advanced cancer.MethodsParticipants (N = 168) completed self‐report assessments of psychosocial, spiritual, and physical well‐being, including social support, optimism, hopelessness, depressive and anxious symptoms, and QOL. Hierarchical multiple regression analyses were conducted to determine the extent to which social support and optimism were associated with depressive and anxious symptomatology, hopelessness and QOL, and the potential role of optimism as a moderator of the relationship between social support and these variables.ResultsHigher levels of optimism were significantly associated with fewer anxious and depressive symptoms, less hopelessness, and better QOL. Higher levels of perceived social support were also significantly associated with better QOL. Additionally, optimism moderated the relationship between social support and anxiety, such that there was a strong negative association between social support and anxiety for participants with low optimism.ConclusionsThis study highlights the importance of optimism and social support in the QOL of patients with advanced cancer. As such, interventions that attend to patients' expectations for positive experiences and the expansion of social support should be the focus of future clinical and research endeavors. Copyright © 2013 John Wiley & Sons, Ltd.