bluuebunny/crossref_metadata_2025_split huggingface dataset:
Initial recommendations for higher-tier risk assessment protocols for bumble bees, Bombus spp. (Hymenoptera: Apidae)
Authors: Cabrera Ana R , Almanza Maria Teresa , Cutler G Christopher , Fischer David L , Hinarejos Silvia , Lewis Gavin , Nigro Daniel , Olmstead Allen , Overmyer Jay , Potter Daniel A , Raine Nigel E , Stanley-Stahr Cory , Thompson Helen , van der Steen Jozef 
Published: June 2015

Abstract
Global declines of bumble bees and other pollinator populations are of concern because of their critical role for crop production and maintenance of wild plant biodiversity. Although the consensus among scientists is that the interaction of many factors, including habitat loss, forage scarcity, diseases, parasites, and pesticides, potentially plays a role in causing these declines, pesticides have received considerable attention and scrutiny. In response, regulatory agencies have introduced more stringent pollinator testing requirements for registration and reregistration of pesticides, to ensure that the risks to pollinators are minimized. In this context, guidelines for testing bumble bees (Bombus spp.) in regulatory studies are not yet available, and a pressing need exists to develop suitable protocols for routine higher-tier studies with these non-Apis sp., social bees. To meet this need, Bayer CropScience LP, Syngenta Crop Protection LLC US, and Valent USA. Corporation organized a workshop bringing together a group of global experts on bumble bee behavior, ecology, and ecotoxicology to discuss and develop draft protocols for both semi-field (Tier II) and field (Tier III) studies. The workshop was held May 8–9, 2014, at the Bayer Bee Care Center, North Carolina, USA. The participants represented academic, consulting, and industry scientists from Europe, Canada, the United States, and Brazil. The workshop identified a clear protection goal and generated proposals for basic experimental designs, relevant measurements, and endpoints for both semifield (tunnel) and field tests. These initial recommendations are intended to form the basis of discussions to help advance the development of appropriate protocol guidelines. Integr Environ Assess Manag 2016;12:222–229. © 2015 The Authors. Integrated Environmental Assessment and Management Published by SETAC
Key Points
Initial recommendations for semifield and field studies with bumble bees based on expert opinions. Identification of a protection goal and relevant endpoints for risk assessment of pesticides to bumble bees. Identification of research needs to advance the development of guidelines for bumble bee regulatory testing.
Electrospinning of PVA/chitosan nanocomposite nanofibers containing gelatin nanoparticles as a dual drug delivery system
Authors: Fathollahipour Shahrzad , Abouei Mehrizi Ali , Ghaee Azadeh , Koosha Mojtaba 
Published: June 2015

AbstractNanofibrous core‐sheath nanocomposite dual drug delivery system based on poly(vinyl alcohol) (PVA)/chitosan/lidocaine hydrochloride loaded with gelatin nanoparticles were successfully prepared by the electrospinning method. Gelatin nanoparticles were prepared by nanoprecipitation and were then loaded with erythromycin antibiotic agent with the average particle size of ∼175 nm. The morphology of gelatin nanoparticles observed by field emission scanning electron microscopy (FE‐SEM) was shown to be optimal at the concentration of 1.25 wt % of gelatin in aqueous phase by addition of 20 µL of glutaraldehyde 5% as the crosslinking agent. The nanoparticles were also characterized by dynamic light scattering, zeta potential measurement, and Fourier transform infrared spectroscopy (FTIR). The best bead free morphology for the PVA/chitosan nanofibrous mats were obtained at the solution weight ratio of 96/4. The nanofibrous mats were analyzed by swelling studies, FTIR and antibacterial tests. In vitro dual release profile of the core‐sheath nanofibers was also studied within 72 h and showed the release efficiency equal to 84.69 and 75.13% for lidocaine hydrochloride and erythromycin, respectively. According to release exponent n, the release of lidocaine hydrochloride from the sheath part of the matrix is quasi‐Fickian diffusion mechanism, while the release of erythromycin is based on anomalous or non‐Fickian mechanisms. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3852–3862, 2015.
Disturbances in Bone Largely Predict Aortic Calcification in an Alternative Rat Model Developed to Study Both Vascular and Bone Pathology in Chronic Kidney Disease
Authors: Neven Ellen , Bashir-Dar Rida , Dams Geert , Behets Geert J , Verhulst Anja , Elseviers Monique , D'Haese Patrick C 
Published: June 2015

ABSTRACT

Because current rat models used to study chronic kidney disease (CKD)-related vascular calcification show consistent but excessive vascular calcification and chaotic, immeasurable, bone mineralization due to excessive bone turnover, they are not suited to study the bone-vascular axis in one and the same animal. Because vascular calcification and bone mineralization are closely related to each other, an animal model in which both pathologies can be studied concomitantly is highly needed. CKD-related vascular calcification in rats was induced by a 0.25% adenine/low vitamin K diet. To follow vascular calcification and bone pathology over time, rats were killed at weeks 4, 8, 10, 11, and 12. Both static and dynamic bone parameters were measured. Vascular calcification was quantified by histomorphometry and measurement of the arterial calcium content. Stable, severe CKD was induced along with hyperphosphatemia, hypocalcemia as well as increased serum PTH and FGF23. Calcification in the aorta and peripheral arteries was present from week 8 of CKD onward. Four and 8 weeks after CKD, static and dynamic bone parameters were measurable in all animals, thereby presenting typical features of hyperparathyroid bone disease. Multiple regression analysis showed that the eroded perimeter and mineral apposition rate in the bone were strong predictors for aortic calcification. This rat model presents a stable CKD, moderate vascular calcification, and quantifiable bone pathology after 8 weeks of CKD and is the first model that lends itself to study these main complications simultaneously in CKD in mechanistic and intervention studies. © 2015 American Society for Bone and Mineral Research.
Osteoclast TGF-β Receptor Signaling Induces Wnt1 Secretion and Couples Bone Resorption to Bone Formation
Authors: Weivoda Megan M , Ruan Ming , Pederson Larry , Hachfeld Christine , Davey Rachel A , Zajac Jeffrey D , Westendorf Jennifer J , Khosla Sundeep , Oursler Merry Jo 
Published: June 2015

ABSTRACT

Osteoblast-mediated bone formation is coupled to osteoclast-mediated bone resorption. These processes become uncoupled with age, leading to increased risk for debilitating fractures. Therefore, understanding how osteoblasts are recruited to sites of resorption is vital to treating age-related bone loss. Osteoclasts release and activate TGF-β from the bone matrix. Here we show that osteoclast-specific inhibition of TGF-β receptor signaling in mice results in osteopenia due to reduced osteoblast numbers with no significant impact on osteoclast numbers or activity. TGF-β induced osteoclast expression of Wnt1, a protein crucial to normal bone formation, and this response was blocked by impaired TGF-β receptor signaling. Osteoclasts in aged murine bones had lower TGF-β signaling and Wnt1 expression in vivo. Ex vivo stimulation of osteoclasts derived from young or old mouse bone marrow macrophages showed no difference in TGF-β–induced Wnt1 expression. However, young osteoclasts expressed reduced Wnt1 when cultured on aged mouse bone chips compared to young mouse bone chips, consistent with decreased skeletal TGF-β availability with age. Therefore, osteoclast responses to TGF-β are essential for coupling bone resorption to bone formation, and modulating this pathway may provide opportunities to treat age-related bone loss. © 2015 American Society for Bone and Mineral Research.
PTH(1-84) Administration in Hypoparathyroidism Transiently Reduces Bone Matrix Mineralization
Authors: Misof Barbara M , Roschger Paul , Dempster David W , Zhou Hua , Bilezikian John P , Klaushofer Klaus , Rubin Mishaela R 
Published: June 2015

ABSTRACT

Patients with hypoparathyroidism have low circulating parathyroid (PTH) levels and higher cancellous bone volume and trabecular thickness. Treatment with PTH(1-84) was shown to increase abnormally low bone remodeling dynamics. In this work, we studied the effect of 1-year or 2-year PTH(1-84) treatment on cancellous and cortical bone mineralization density distribution (Cn.BMDD and Ct.BMDD) based on quantitative backscattered electron imaging (qBEI) in paired transiliac bone biopsy samples. The study cohort comprised 30 adult hypoparathyroid patients (14 treated for 1 year; 16 treated for 2 years). At baseline, Cn.BMDD was shifted to higher mineralization densities in both treatment groups (average degree of mineralization Cn.CaMean +3.9% and +2.7%, p &lt; 0.001) compared to reference BMDD. After 1-year PTH(1-84), Cn.CaMean was significantly lower than that at baseline (–6.3%, p &lt; 0.001), whereas in the 2-year PTH(1-84) group Cn.CaMean did not differ from baseline. Significant changes of Ct.BMDD were observed in the 1-year treatment group only. The change in histomorphometric bone formation (mineralizing surface) was predictive for Cn.BMDD outcomes in the 1-year PTH(1-84) group, but not in the 2-year PTH(1-84) group. Our findings suggest higher baseline bone matrix mineralization consistent with the decreased bone turnover in hypoparathyroidism. PTH(1-84) treatment caused differential effects dependent on treatment duration that were consistent with the histomorphometric bone formation outcomes. The greater increase in bone formation during the first year of treatment was associated with a decrease in bone matrix mineralization, suggesting that PTH(1-84) exposure to the hypoparathyroid skeleton has the greatest effects on BMDD early in treatment. © 2015 American Society for Bone and Mineral Research.
Stimulation of Cell Migration by Flagellin Through the p38 MAP Kinase Pathway in Cultured Intestinal Epithelial Cells
Authors: Kondo Yutaka , Higa‐Nakamine Sayomi , Maeda Noriko , Toku Seikichi , Kakinohana Manabu , Sugahara Kazuhiro , Kukita Ichiro , Yamamoto Hideyuki 
Published: June 2015

ABSTRACTToll‐like receptor 5 (TLR5) is a receptor for flagellin and is present on the basolateral surface of intestinal epithelial cells. However, the pathological roles of TLR5 in intestinal epithelial cells are not clear at present. In previous reports, we demonstrated that treatment of cultured alveolar epithelial cells with flagellin activated the p38 mitogen‐activated protein kinase (MAPK) pathway and enhanced epithelial‐mesenchymal transition induced by transforming growth factor beta 1 (TGF‐β1). In translating our findings in alveolar epithelial cells to intestinal epithelial cells, we found that both flagellin and TGF‐β1 activated p38 MAPK and its downstream protein kinase, MAPK‐activated protein kinase‐2 (MAPKAPK‐2) in an IEC‐6 intestinal epithelial cell line. The phosphorylation of HSP27, one of the substrates for MAPKAPK‐2, was also increased. TGF‐β1 increased the protein level of α‐smooth muscle actin (αSMA), and flagellin enhanced the effect of TGF‐β1. A wound healing assay revealed that flagellin and TGF‐β1 stimulated the migration of cells. SB203580, an inhibitor of p38 MAPK, and an inhibitor of MAPKAPK‐2 inhibited flagellin‐stimulated migration. These results suggested that TLR5 is involved in the migration of intestinal epithelial cells through activation of the p38 MAPK pathway. J. Cell. Biochem. 117: 247–258, 2016. © 2015 Wiley Periodicals, Inc.
Automated analysis protocol for high resolution BOLD‐fMRI mapping of the fingertip somatotopy in brodmann area 3b
Authors: Pfannmöller Jörg P. , Schweizer Renate , Lotze Martin 
Published: June 2015

BackgroundTo introduce a standardized and automatized method for functional MRI (fMRI) examinations of the cortical sensory somatotopy in large samples for investigations of the fingertip somatotopy in the primary somatosensory cortex.MethodsAt 3 Tesla, T2* (spin‐spin relaxation time) weighted images (gradient‐echo echo planar imaging, voxel size 1.5 × 1.5 × 2 mm3) were acquired during stimulation of the finger tips for thumb, index and middle finger on both hands, in a group of 18 healthy participants. In addition, structural T1 weighted (magnetization prepared rapid gradient echo, isotropic voxel size 1 mm) and MR‐angiography (time of flight, voxel size 0.26 × 0.26 × 0.5 mm3) images were recorded. Boundary based register served to combine movement correction and registration in FreeSurfer Functional analysis stream (FS‐Fast), resulting in fine scale corrections, as revealed with FSL Possum (FSL FMRIB Software Library Physics‐Oriented Simulated Scanner for Understanding MRI) simulations. Automated data analysis was achieved by inclusion of cytoarchitectonic probability maps for calculation of functional activation in Brodmann area 3b. Draining vessel artifacts were identified using the peak value approach and the MR‐angiography. Distances were computed as the shortest connection within the gray matter.ResultsThe fMRI somatotopic maps agreed with the expected fingertip somatotopy in 63% of the investigated subjects, an improvement of 34% compared with FS‐Fast. Artifacts have been removed completely. Adjacent fingertips showed average distances of 8 ± 4.3 mm, and between thumb and middle finger 13.4 ± 4.8 mm was found. Distances for both hands were similar as expected from the characteristics of the fingertip spatial tactile resolution.ConclusionThe introduced evaluation procedure allowed automated analysis of the fingertip representation in excellent agreement with preceding results. J. Magn. Reson. Imaging 2016;43:479–486.
Growth, yield, and yield components of canola as affected by nitrogen, sulfur, and boron application
Authors: Ma Bao‐Luo , Biswas Dilip K. , Herath Aruna W. , Whalen Joann K. , Ruan S. Qianying , Caldwell Claude , Earl Hugh , Vanasse Anne , Scott Peter , Smith Donald L. 
Published: June 2015

AbstractDeveloping efficient nutrient management regimes is a prerequisite for promoting canola (Brassica napus L.) as a viable cash crop in eastern Canada. Field experiments were conducted to investigate the growth, yield, and yield components of canola in response to various combinations of preplant and sidedress nitrogen (N) with soil‐applied sulfur (S) and soil and foliar‐applied boron (B). Canola yield and all its yield components were strongly correlated (r2 = 0.99) with the amount of N applied, as was the above‐ground biomass at 20% flowering and the leaf area index. Sidedress N was more efficiently utilized by the crop, leading to greater yields than preplant N application. On average, canola yields increased by 9.7 kg ha−1 for preplant N application and by 13.7 kg ha−1 for sidedress N application, for every kg N ha−1 applied, in 6 of the 10 site‐years. Soil‐applied S also increased canola yields by 3–31% in 7 of the 10 site‐years, but had no effect on yield components. While there was no change in yield from soil‐applied B, the foliar B application at early flowering increased yields up to 10%, indicating that canola plants absorb B efficiently through their leaves. In summary, canola yields were improved by fertilization with N (8 of 10 site‐years), S (7 of 10 site‐years) and B (4 of 10 site‐years). Yield gains were also noted with split N‐fertilizer application that involved sidedressing N between the rosette and early flowering stage. Following these fertilizer practices could improve the yield and quality of canola crop grown in rainfed humid regions similar to those in eastern Canada.
Phospholipids in foods: prooxidants or antioxidants?
Authors: Cui Leqi , Decker Eric A 
Published: June 2015

AbstractLipid oxidation is one of the major causes of quality deterioration in natural and processed foods and thus a large economic concern in the food industry. Phospholipids, especially lecithins, are already widely used as natural emulsifiers and have been gaining increasing interest as natural antioxidants to control lipid oxidation. This review summarizes the fatty acid composition and content of phospholipids naturally occurring in several foods. The role of phospholipids as substrates for lipid oxidation is discussed, with a focus on meats and dairy products. Prooxidant and antioxidant mechanisms of phospholipids are also discussed to get a better understanding of the possible opportunities for using phospholipids as food antioxidants. © 2015 Society of Chemical Industry
Mapping Extent and Change in Surface Mines Within the United States for 2001 to 2006
Authors: Soulard Christopher E. , Acevedo William , Stehman Stephen V. , Parker Owen P. 
Published: June 2015

AbstractA complete, spatially explicit dataset illustrating the 21st century mining footprint for the conterminous United States does not exist. To address this need, we developed a semi‐automated procedure to map the country's mining footprint (30‐m pixel) and establish a baseline to monitor changes in mine extent over time. The process uses mine seed points derived from the U.S. Energy Information Administration (EIA), U.S. Geological Survey (USGS) Mineral Resources Data System (MRDS), and USGS National Land Cover Dataset (NLCD) and recodes patches of barren land that meet a “distance to seed” requirement and a patch area requirement before mapping a pixel as mining. Seed points derived from EIA coal points, an edited MRDS point file, and 1992 NLCD mine points were used in three separate efforts using different distance and patch area parameters for each. The three products were then merged to create a 2001 map of moderate‐to‐large mines in the United States, which was subsequently manually edited to reduce omission and commission errors. This process was replicated using NLCD 2006 barren pixels as a base layer to create a 2006 mine map and a 2001–2006 mine change map focusing on areas with surface mine expansion. In 2001, 8,324 km2 of surface mines were mapped. The footprint increased to 9,181 km2 in 2006, representing a 10·3% increase over 5 years. These methods exhibit merit as a timely approach to generate wall‐to‐wall, spatially explicit maps representing the recent extent of a wide range of surface mining activities across the country. Copyright © 2015 John Wiley & Sons, Ltd.
Can macroalgae contribute to blue carbon? An Australian perspective
Authors: Hill Ross , Bellgrove Alecia , Macreadie Peter I. , Petrou Katherina , Beardall John , Steven Andy , Ralph Peter J. 
Published: June 2015

AbstractMacroalgal communities in Australia and around the world store vast quantities of carbon in their living biomass, but their prevalence of growing on hard substrata means that they have limited capacity to act as long‐term carbon sinks. Unlike other coastal blue carbon habitats such as seagrasses, saltmarshes and mangroves, they do not develop their own organic‐rich sediments, but may instead act as a rich carbon source and make significant contributions in the form of detritus to sedimentary habitats by acting as a “carbon donor” to “receiver sites” where organic material accumulates. The potential for storage of this donated carbon however, is dependent on the decay rate during transport and the burial efficiency at receiver sites. To better understand the potential contribution of macroalgal communities to coastal blue carbon budgets, a comprehensive literature search was conducted using key words, including carbon sequestration, macroalgal distribution, abundance and productivity to provide an estimation of the total amount of carbon stored in temperate Australian macroalgae. Our most conservative calculations estimate 109.9 Tg C is stored in living macroalgal biomass of temperate Australia, using a coastal area covering 249,697 km2. Estimates derived for tropical and subtropical regions contributed an additional 23.2 Tg C. By extending the search to include global studies we provide a broader context and rationale for the study, contributing to the global aspects of the review. In addition, we discuss the potential role of calcium carbonate‐containing macroalgae, consider the dynamic nature of macroalgal populations in the context of climate change, and identify the knowledge gaps that once addressed will enable robust quantification of macroalgae in marine biogeochemical cycling of carbon. We conclude that macroalgal communities have the potential to make ecologically meaningful contributions toward global blue carbon sequestration, as donors, but given that the fate of detached macroalgal biomass remains unclear, further research is needed to quantify this contribution.
Potential Clinical Uses of CDK Inhibitors: Lessons from Synthetic Lethality Screens
Authors: Vymětalová Ladislava , Kryštof Vladimír 
Published: June 2015

AbstractDevelopments in genetic and genomic technology have produced vast quantities of data that are gradually yielding new insights into fundamental cellular and molecular processes. In particular, they have revealed some differences between normal and transformed cells that could potentially be exploited to develop targeted, personalized cancer therapies with unprecedented efficiencies. This review summarizes recent findings from synthetic lethality (SL) screens against cyclin‐dependent kinases (CDKs) that can be targeted with small molecule kinase inhibitors. SL screens can be used to identify cancers sensitive to CDK inhibitors. Several SL partners of specific CDKs have been identified, including MYC, K‐Ras, VHL, PI3K, and PARP, all of which are discussed in the review. CDK inhibitors have been in clinical trials for nearly 20 years and it has become clear that effective therapy using these compounds will require careful selection of patients with respect to the specific molecular phenotype of their disease.
Repetitive nerve stimulation and single‐fiber electromyography in the evaluation of patients with suspected myasthenia gravis or Lambert–Eaton myasthenic syndrome: Review of recent literature
Authors: Chiou‐Tan Faye Y. , Gilchrist James M. 
Published: June 2015

ABSTRACTIntroduction: Our aim in this study was to provide an updated literature review of electrodiagnostic testing in myasthenia gravis and Lambert–Eaton myasthenic syndrome. Methods: A systematic review of the recent literature was performed using the following key words: myasthenia gravis (MG); Lambert–Eaton myasthenic syndrome (LEMS); electromyography (EMG); repetitive nerve stimulation (RNS); single‐fiber electromyography (SFEMG); nerve conduction study; and normative values. Results: Several articles supported testing of facial, bulbar, and respiratory muscles in the diagnosis of neuromuscular junction (NMJ) disorders, including muscle‐specific kinase antibody (MuSK)‐seropositive MG. Several articles supported use of concentric needle EMG as an alternative to SFEMG jitter in disorders of neuromuscular transmission. A limited number of articles addressed measurement of area (vs. amplitude) decrement in RNS and decreasing the threshold of post‐exercise facilitation. Conclusions: Electrodiagnostic testing continues to be useful for diagnosis of MG and LEMS, although the quality of the evidence is not great. This literature review summarizes RNS and jitter measurement of facial and respiratory muscles and use of concentric needle EMG for SFEMG. Muscle Nerve 52:455–462, 2015