bluuebunny/crossref_metadata_2025_split huggingface dataset:
Macrophage‐mediated heart repair and remodeling: A promising therapeutic target for post‐myocardial infarction heart failure
Authors: Yin Wenchao , Chen Yong , Wang Wenjun , Guo Mengqi , Tong Lingjun , Zhang Mingxiang , Wang Zhaoyang , Yuan Haitao 
Published: July 2024

AbstractHeart failure (HF) remains prevalent in patients who survived myocardial infarction (MI). Despite the accessibility of the primary percutaneous coronary intervention and medications that alleviate ventricular remodeling with functional improvement, there is an urgent need for clinicians and basic scientists to further reveal the mechanisms behind post‐MI HF as well as investigate earlier and more efficient treatment after MI. Growing numbers of studies have highlighted the crucial role of macrophages in cardiac repair and remodeling following MI, and timely intervention targeting the immune response via macrophages may represent a promising therapeutic avenue. Recently, technology such as single‐cell sequencing has provided us with an updated and in‐depth understanding of the role of macrophages in MI. Meanwhile, the development of biomaterials has made it possible for macrophage‐targeted therapy. Thus, an overall and thorough understanding of the role of macrophages in post‐MI HF and the current development status of macrophage‐based therapy will assist in the further study and development of macrophage‐targeted treatment for post‐infarction cardiac remodeling. This review synthesizes the spatiotemporal dynamics, function, mechanism and signaling of macrophages in the process of HF after MI, as well as discusses the emerging bio‐materials and possible therapeutic agents targeting macrophages for post‐MI HF.
Ineffectiveness of phosphorus‐containing amendments to reduce Pb bioaccessibility in an urban alkaline soil
Authors: Lake Loryssa , Basta Nicholas 
Published: July 2024

AbstractUrban soils contaminated by historical and current anthropogenic activities present an alarming human health risk requiring redress. Federal and state governments continue to lower residential soil lead (Pb) screening standards, which will likely require new risk‐based approaches to address urban soil Pb contamination. Phosphorus (P) soil amendments have long been presented as a solution to sequester Pb, thereby reducing exposure risk. In this study, P‐containing sources (biosolids incinerator ash, poultry litter, biosolids compost, and triple superphosphate) of varying solubilities were assessed as soil amendments to reduce Pb bioaccessibility and serve as an inexpensive remediation strategy for urban soil. Contaminated soil (1624 mg kg−1 Pb, pH 7.43) from Cleveland, OH, was treated with the four P‐containing soil amendments at a 1:5 Pb:P molar ratio and two combination treatments at 1:10 Pb:P molar ratio and incubated for 3 months. A batch equilibration analysis was also conducted to assess reduction in in vitro bioaccessible Pb (IVBA Pb). Pb bioaccessibility was evaluated using US EPA Method 1340 at pH 1.5 and the Physiologically Based Extraction Test pH 2.5 at 1 and 3 months. In general, treatments were ineffective in reducing IVBA Pb regardless of IVBA extraction method, incubation duration, batch equilibration analyses, or P source. The results of this study suggest P‐containing amendments are not suitable to address Pb exposure in the study soil. Site‐specific efficacy testing to determine reductions in IVBA Pb from P‐containing amendments should be performed before making recommendations for remediation of Pb‐contaminated urban soil.
Microbial diversity and gene abundance in denitrifying bioreactors: A comparison of the woodchip surface biofilm versus the interior wood matrix
Authors: Duggan DiDominic Katie L. , Shapleigh James P. , Walter M. Todd , Wang Y. Samuel , Reid Matthew C. , Regan John M. 
Published: July 2024

AbstractExcessive amounts of nitrogen (N) and phosphorus (P) can lead to eutrophication in water sources. Woodchip bioreactors have shown success in removing N from agricultural runoff, but less is known regarding P removal. Woodchip bioreactors are subsurface basins filled with woodchips installed downgradient of agricultural land to collect and treat drainage runoff. Microorganisms use the woodchips as a carbon (C) source to transform N in the runoff, with unresolved biological impacts on P. This study aims to explore microbial communities present in the bioreactor and determine whether milling woodchips to probe the microbial communities within them reveals hidden microbial diversities or potential activities. Metagenomic sequencing and bioinformatic analyses were performed on six woodchip samples (i.e., three unmilled and three milled) collected from a 10‐year‐old woodchip bioreactor treating agricultural tile drainage. All samples had similar DNA purity, yield, quality, and microbial diversity regardless of milling. However, when sequences were aligned against various protein libraries, our results indicated greater relative abundance of denitrification and P transformation proteins on the outside of the woodchips (unmilled), while the interior of woodchips (milled) exhibited more functional gene abundance for carbohydrate breakdown. Thus, it may be important to characterize microbial communities both within woodchips, and on woodchip surfaces, to gain a more holistic understanding of coupled biogeochemical cycles on N, P, and C in woodchip bioreactors. Based on these findings, we advise that future microbial research on woodchips (and potentially other permeable organic materials) examine both the surface biofilm and the interior organic material during initial studies. Once researchers determine where specific proteins or enzymes of interest are most prevalent, subsequent studies may then focus on either one or both aspects, as needed.
Comparative osteology of the skull of cowbirds (Icteridae: Molothrus)
Authors: Gómez Raúl O. , Lois‐Milevicich Jimena 
Published: July 2024

AbstractDetailed osteological descriptions of the craniomandibular complex of passerine birds are lacking for most species, limiting our understanding of their diversity and evolution. Cowbirds (genus Molothrus) are a small but widespread group of New World nine‐primaried songbirds, well‐known for their unique brooding parasitic behavior. However, detailed osteological data for cowbirds and other Icteridae are currently scarce and several features of their skulls remain undescribed or poorly known. To address this issue, a detailed comparative osteology of cowbird skulls is presented here for the first time based on data from x‐ray microcomputed tomography, dry skeletal data, and multivariate analyses of linear morphometric data. Cowbird skulls offer some functional insights, with many finch‐like features probably related to a seed‐rich diet that distinguishes them from most other icterids. In addition, features previously overlooked in earlier studies might provide valuable phylogenetic information at different levels of passerine phylogeny (Passerida, Emberizoidea, Icteridae, and Agelaiinae), including some of the otic region and nasal septum. Comparisons among cowbirds show that there is substantial cranial variation within the genus, with M. oryzivorus being the most divergent cowbird species. Within the genus, distantly related species share similar overall skull morphology and proportions, but detailed osteological data allow species identification even in cases of strong convergence. Further efforts are warranted to furnish baseline data for future studies of this iconic group of Neotropical birds and to fully integrate it into phylogenetic comparative frameworks.
Multi‐modal mass spectrometry imaging of a single tissue section
Authors: Zhan Lingpeng , Huang Yanyi , Wang Guanbo 
Published: July 2024

AbstractMass spectrometry imaging (MSI) was developed to visualize spatial chemical information within tissues, thereby facilitating spatial multi‐omic analysis. However, due to the limited spatial information provided by individual modal MSI, correlating various chemical data within tissues remains a significant challenge. In recent years, multimodal MSI has garnered considerable attention due to its ability to visualize the spatial distributions of multiple biomolecules within tissues. Among the strategies employed in this field, multimodal imaging on a single tissue section circumvents multiple issues introduced by integration of images of consecutive tissue sections. In this minireview, we provide an overview of multimodal MSI on a single tissue section, with a particular focus on the use of Matrix‐Assisted Laser Desorption/Ionization–MSI for spatial multi‐omic investigations that offer a comprehensive and in‐depth elucidation of the biological state and activities, aiming to inspire the development of new approaches in this field.
Metagenomic analysis reveals altered gut virome and diagnostic potential in pancreatic cancer
Authors: Zhang Pan , Shi Haitao , Guo Ruochun , Li Lu , Guo Xiaoyan , Yang Hui , Chang Danyan , Cheng Yan , Zhao Gang , Li Shenghui , Zhong Qingling , Zhang Huan , Zhao Ping , Fu Cui , Song Yahua , Yang Longbao , Wang Yan , Zhang Yue , Jiang Jiong , Wang Ting , Zhao Juhui , Li Yong , Wang Biyuan , Chen Fenrong , Zhao Hongli , Wang Yonghua , Wang Jinhai , Ma Shiyang 
Published: July 2024

AbstractPancreatic cancer (PC) is a highly aggressive malignancy with a poor prognosis, making early diagnosis crucial for improving patient outcomes. While the gut microbiome, including bacteria and viruses, is believed to be essential in cancer pathogenicity, the potential contribution of the gut virome to PC remains largely unexplored. In this study, we conducted a comparative analysis of the gut viral compositional and functional profiles between PC patients and healthy controls, based on fecal metagenomes from two publicly available data sets comprising a total of 101 patients and 82 healthy controls. Our results revealed a decreasing trend in the gut virome diversity of PC patients with disease severity. We identified significant alterations in the overall viral structure of PC patients, with a meta‐analysis revealing 219 viral operational taxonomic units (vOTUs) showing significant differences in relative abundance between patients and healthy controls. Among these, 65 vOTUs were enriched in PC patients, and 154 were reduced. Host prediction revealed that PC‐enriched vOTUs preferentially infected bacterial members of Veillonellaceae, Enterobacteriaceae, Fusobacteriaceae, and Streptococcaceae, while PC‐reduced vOTUs were more likely to infect Ruminococcaceae, Lachnospiraceae, Clostridiaceae, Oscillospiraceae, and Peptostreptococcaceae. Furthermore, we constructed random forest models based on the PC‐associated vOTUs, achieving an optimal average area under the curve (AUC) of up to 0.879 for distinguishing patients from controls. Through additional 10 public cohorts, we demonstrated the reproducibility and high specificity of these viral signatures. Our study suggests that the gut virome may play a role in PC development and could serve as a promising target for PC diagnosis and therapeutic intervention. Future studies should further explore the underlying mechanisms of gut virus‐bacteria interactions and validate the diagnostic models in larger and more diverse populations.
TTV and CMV viral load dynamics: Which emerges first during immunosuppression?
Authors: Roberto Piergiorgio , Cinti Lilia , Lucente Dario , Russo Gianluca , Lai Quirino , Micozzi Alessandra , Gentile Giuseppe , Turriziani Ombretta , Pierangeli Alessandra , Antonelli Guido 
Published: July 2024

AbstractNovel biomarkers reflecting the degree of immunosuppression in transplant patients are required to ensure eventual personalized equilibrium between rejection and infection risks. With the above aim, Torque Teno Virus (TTV) viremia was precisely examined in a large cohort of transplanted immunocompromised patients (192 hematological and 60 solid organ transplant recipients) being monitored for Cytomegalovirus reactivation. TTV load was measured in 2612 plasma samples from 448 patients. The results revealed a significant increase in TTV viral load approximately 14 days following CMV reactivation/infection in solid organ transplant (SOT) patients. No recognizable difference in TTV load was noted among hematological patients during the entire timeframe analyzed. Furthermore, a temporal gap of approximately 30 days was noted between the viral load peaks reached by the two viruses, with Cytomegalovirus (CMV) preceding TTV. It was not possible to establish a correlation between CMV reactivation/infection and TTV viremia in hematological patients. On the other hand, the SOT patient cohort allowed us to analyze viral kinetics and draw intriguing conclusions. Taken together, the data suggest, to our knowledge for the first time, that CMV infection itself could potentially cause an increase in TTV load in the peripheral blood of patients undergoing immunosuppressive therapy.
Clinical performance of Circulating HBV RNA and iTACT‐HBcrAg Assays in HBeAg‐negative and HBsAg‐cleared Chronic Hepatitis B Patients
Authors: Suzuki Takanori , Matsuura Kentaro , Inoue Takako , Sasada Keiko , Ogawa Shintaro , Watanabe Takehisa , Kawamura Hayato , Fujiwara Kei , Kataoka Hiromi , Tanaka Yasuhito 
Published: July 2024

AbstractHepatitis B virus (HBV) RNA and hepatitis B core‐related antigen (HBcrAg) have been reported to reflect the transcriptional activity of covalently closed circular HBV DNA. We retrospectively investigated the proportions of quantifiable serum HBV RNA and immunoassay for total antigen including complex via pretreatment‐hepatitis B core‐related antigen (iTACT‐HBcrAg) in chronic hepatitis B patients negative for hepatitis B e antigen (HBeAg) and/or with hepatitis B surface antigen (HBsAg) seroclearance. This study included 246 HBeAg‐negative HBV‐infected patients, who comprised 13 with liver cirrhosis (LC, the LC group), 118 chronic hepatitis (CH, the CH group), and 115 inactive carriers (IC, the IC group), and 44 patients with HBsAg seroclearance. iTACT‐HBcrAg and HBV RNA levels were determined using stored serum samples. Higher proportions of the patients had quantifiable iTACT‐HBcrAg than HBV RNA in all groups of HBeAg‐negative patients (iTACT‐HBcrAg: 84.6%, 90.7%, 35.7%, HBV RNA: 23.1%, 26.3%, 14.8%, for the LC, CH, IC groups). With HBsAg seroclearance (HBsAg <0.05 IU/mL), the proportions of quantifiable samples for HBV RNA were also lower than iTACT‐HBcrAg (0% for HBV RNA). Thus, iTACT‐HBcrAg was more often detectable than circulating HBV RNA in this study population. Further long‐term prospective evaluation of iTACT‐HBcrAg is desirable for its utilization in clinical practice.
The effects of eWom triggered service recovery on customer citizenship behavior in the hospitality industry: The moderating role of failure severity
Authors: Tengilimoglu Engin , Öztürk Yüksel 
Published: July 2024

AbstractThis study examines service failures in the hospitality industry, amplified by the shift to online platforms, and highlights the need for effective online recovery methods. These methods are crucial for retaining existing customers and preventing potential customer loss. Despite its importance, customer citizenship behavior resulting from service recovery must be noticed. This study examines the impact of service failure severity and online recovery methods on customer citizenship behavior, employing six scenarios varying in severity and recovery strategies. Multi‐group scanning electron microscope analysis reveals that justice types positively influence recovery satisfaction, enhancing customer citizenship behaviors. Failure severity moderates the relationship between recovery satisfaction and customer citizenship behavior, with recovery satisfaction being more effective in promoting advocacy and tolerance behaviors during low‐severity failures. However, this effect diminishes in high‐severity failures. Effective recovery strategies can transform negative service failures into positive outcomes for hospitality organizations.
Exploring the formation of destination loyalty from destination personality and self‐congruity: A mediating role of destination relationship quality
Authors: Li Mao‐Hua , Lai Ivan Ka Wai 
Published: July 2024

AbstractThis study proposes a new concept of the tourist‐destination relationship called destination relationship quality. It consists of four elements: destination satisfaction, destination trust, destination commitment, and destination attachment. The study investigates how destination personality and self‐congruity influence tourists' destination loyalty through destination relationship quality. Data were obtained through systematic sampling from 424 Chinese tourists in Guangzhou, China. Using partial least squares structural equation modelling analysis, this study confirmed that destination personality and self‐congruity are antecedent factors of destination relationship quality, which ultimately affect tourists' destination loyalty towards a destination. The study contributes to tourist‐destination relationship research by indicating the mechanism for establishing destination relationship quality so that researchers can further apply this concept in their future studies. The results of this study are helpful for tourist destination marketers to attach importance to the establishment of destination personality and create core differences in tourist destinations when developing marketing strategies.
Prospective Evaluation of Normal Supraspinatus Muscle Using Shear Wave Elastography
Authors: Jeong Jeung Yeol , Khil Eun Kyung , Seo Woorim 
Published: July 2024

ObjectiveThis study aimed to evaluate normal supraspinatus (SS) muscle elasticity using shear wave elastography (SWE) in an asymptomatic group, analyze its correlation with demographic factors and ultrasound (US) grayscale grade, and compare the elasticity between normal SS muscles and symptomatic SS muscles with tendon tears.MethodsA prospective study was conducted with 101 adult patients with normal SS muscle scheduled for surgery due to a contralateral shoulder with SS tendon tear. Both shoulders underwent US examinations, including SWE. The SWE values, including mean and median elasticity, as well as the elasticity ratio, were analyzed for their correlation with demographic characteristics and grayscale grades. A comparison of SWE values was performed between the normal and symptomatic SS muscle groups.ResultsThe mean SWE modulus of the normal SS muscle was 27.87 kPa (±49.04), with an elasticity ratio of approximately 1.52 (±0.03). Males exhibited slightly higher elasticity ratios compared with females (1.61 vs 1.45, P = .016). The interobserver agreement for all SWE measurements was excellent (>0.8). Grayscale grade increased with age, showing a similar pattern in females (P < .001). However, no significant correlation was observed between SWE values and grayscale grade in the normal SS muscle group. SWE values in normal SS muscles were significantly lower than those with tendon tears (P < .001).ConclusionsSWE provides objective measurements of normal SS muscle elasticity. Gender‐based variations were observed, with males exhibiting slightly higher elastography ratios. SWE values were significantly lower in asymptomatic SS muscles compared with those with tendon tears.
Trends and epidemiology in robotic‐assisted total knee arthroplasty: Reduced complications and shorter hospital stays
Authors: Maman David , Laver Lior , Becker Roland , Takrori Loai Ahmad , Mahamid Assil , Finkel Binyamin , Gan‐Or Hadar , Yonai Yaniv , Berkovich Yaron 
Published: July 2024

AbstractIntroductionThis study provides an in‐depth analysis of the immediate postoperative outcomes and implications or robotic‐assisted total knee arthroplasty (RA‐TKA) compared with conventional TKA (C‐TKA), particularly with regard to mortality, complications, hospital stay and costs, drawing from a comprehensive nationwide data set.MethodsThe Nationwide Inpatient Sample (NIS) database, the largest all‐payer inpatient healthcare database in the United States, was used to identify all patients who underwent RA‐TKA or C‐TKA from 2016 to 2019. A total of 527,376 cases, representing 2,638,679 patients who underwent elective TKA were identified, of which 88,415 had RA‐TKA. To mitigate potential variations and selection bias in baseline characteristics between the two groups, a propensity score‐matched analysis was employed to further balance and refine our data set, resulting in 176,830 patients evenly distributed between the groups. Analysis was performed according to demographics, immediate post‐operative complications, and economic data, including payor class, length of stay and total charges.ResultsThere was a marked shift towards RA‐TKA, from an initial 0.70% in 2016 to a notable 7.30% by 2019. Patients who underwent RA‐TKA were slightly younger (66.2 ± SD years), compared to the C‐TKA group (66.7 ± SD years). Hospital stay was 1.89 days and 2.29 days for RA‐TKA and C‐TKA, respectively. Charges metrics revealed slightly higher charges for RA‐TKA. Less postoperative complications were found in the RA‐TKA group, such as blood loss, anaemia, acute kidney injury, venous thromboembolism, pulmonary embolism, pneumonia and surgical wound complication. Even following the propensity score matching, these findings remained consistent and statistically significant.ConclusionsRA‐TKA use in the United States has grown substantially in the last few years and has been associated with significantly reduced immediate post‐operative complications and length of hospital stay compared to C‐TKA, offering safer surgical management for TKA patients. Further studies on the short‐ and long‐term outcomes of RA‐TKA would improve the understanding of the full potential of this technology.Levels of EvidenceLevel III.
Hamstring autografts demonstrate either similar or inferior outcomes to quadriceps or bone–patellar tendon–bone autografts in revision anterior cruciate ligament reconstruction: A systematic review of comparative studies
Authors: Vivekanantha Prushoth , Kahlon Harjind , Hassan Zackariyah , Slawaska‐Eng David , Abdel‐Khalik Hassaan , Johnson Jansen , de SA Darren 
Published: July 2024

ABSTRACTPurposeTo evaluate the clinical outcomes in patients undergoing revision anterior cruciate ligament reconstruction (r‐ACLR) using hamstring tendon (HT) autografts with those using either quadriceps tendon (QT) or bone–patellar tendon–bone (BPTB) autografts or allografts.MethodsThree databases were searched on 8 August 2023. The authors adhered to the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) and Revised Assessment of Multiple Systematic Reviews (R‐AMSTAR) guidelines and the Cochrane Handbook for Systematic Reviews of Interventions. Data on demographics, surgical details, patient‐reported outcome measures (PROMs), rates of instability, failure and return to sport (RTS) were extracted.ResultsEleven studies comprising 859 patients were included in this review. Five studies compared HT and QT autografts. One study each reported greater IKDC scores (p = 0.04) and Tegner scores (p = 0.04) in the QT group, while one study each reported higher anterior translation (p = 0.04), rates of positive pivot shift (p = 0.03) and rates of failure (p = 0.03) in the HT group. Six studies compared HT and BPTB autografts with one study each reporting greater Lysholm scores (p = 0.02) and less side‐to‐side anterior laxity (p < 0.01) in the BPTB group. Two studies compared HT autografts with allografts with only one study reporting a faster time to RTS in the HT group than the allograft group (p < 0.001). All other comparisons were not significant.ConclusionsHT autografts result in either similar or inferior outcomes in r‐ACLR when compared to QT or BPTB autograft options. Allografts resulted in similar outcomes to HT autografts apart from greater time to RTS for r‐ACLR.Level of EvidenceLevel III.