bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dual-emissive Polymer Dots for Rapid Detection of Fluoride in Pure Water and Biological Systems with Improved Reliability and Accuracy
Authors: Zhao Qiang , Zhang Chuanqi , Liu Shujuan , Liu Yahong , Zhang Kenneth Yin , Zhou Xiaobo , Jiang Jiayang , Xu Wenjuan , Yang Tianshe , Huang Wei 
Published: November 2015

AbstractIt is of paramount importance to develop new probes that can selectively, sensitively, accurately and rapidly detect fluoride in aqueous media and biological systems, because F- is found to be closely related to many health and environmental concerns. Herein, a dual-emissive conjugated polyelectrolyte P1 containing phosphorescent iridium(III) complex was designed and synthesized, which can form ultrasmall polymer dots (Pdots) in aqueous media. The F--responsive tert-butyldiphenylsilyl moiety was introduced into iridium(III) complex as the signaling unit for sensing F− with the quenched phosphorescence. Thus, the dual-emissive Pdots can rapidly and accurately detect F− in aqueous media and live cells as a ratiometric probe by measuring the change in the ratio of the F−-sensitive red phosphorescence from iridium(III) complex to the F−-insensitive blue fluorescence from polyfluorene. Moreover, the interaction of Pdots with F− also changes its emission lifetime and the lifetime-based detection of F− in live cells has been realized through photoluminescence lifetime imaging microscopy for the first time. Both the ratiometric luminescence and lifetime imaging have been demonstrated to be resistant to external influences, such as the probe’s concentration and excitation power. This study provides a new perspective for the design of promising Pdots-based probes for biological applications.
Doubly curved nanofiber-reinforced optically transparent composites
Authors: Shams Md. Iftekhar , Yano Hiroyuki 
Published: November 2015

AbstractDoubly curved nanofiber-reinforced optically transparent composites with low thermal expansion of 15 ppm/k are prepared by hot pressing vacuum-filtered Pickering emulsions of hydrophobic acrylic resin monomer, hydrophilic chitin nanofibers and water. The coalescence of acrylic monomer droplets in the emulsion is prevented by the chitin nanofibers network. This transparent composite has 3D shape moldability, making it attractive for optical precision parts.
A comprehensive analysis of the chorion locus in silkmoth
Authors: Chen Zhiwei , Nohata Junko , Guo Huizhen , Li Shenglong , Liu Jianqiu , Guo Youbing , Yamamoto Kimiko , Kadono-Okuda Keiko , Liu Chun , Arunkumar Kallare P. , Nagaraju Javaregowda , Zhang Yan , Liu Shiping , Labropoulou Vassiliki , Swevers Luc , Tsitoura Panagiota , Iatrou Kostas , Gopinathan Karumathil P. , Goldsmith Marian R. , Xia Qingyou , Mita Kazuei 
Published: November 2015

AbstractDespite more than 40 years of intense study, essential features of the silkmoth chorion (eggshell) are still not fully understood. To determine the precise structure of the chorion locus, we performed extensive EST analysis, constructed a bacterial artificial chromosome (BAC) contig and obtained a continuous genomic sequence of 871,711 base pairs. We annotated 127 chorion genes in two segments interrupted by a 164 kb region with 5 non-chorion genes, orthologs of which were on chorion bearing scaffolds in 4 ditrysian families. Detailed transcriptome analysis revealed expression throughout choriogenesis of most chorion genes originally categorized as “middle” and evidence for diverse regulatory mechanisms including cis-elements, alternative splicing and promoter utilization and antisense RNA. Phylogenetic analysis revealed multigene family associations and faster evolution of early chorion genes and transcriptionally active pseudogenes. Proteomics analysis identified 99 chorion proteins in the eggshell and micropyle localization of 1 early and 6 Hc chorion proteins.
ADAM9 enhances CDCP1 protein expression by suppressing miR-218 for lung tumor metastasis
Authors: Chiu Kuo-Liang , Kuo Ting-Ting , Kuok Qian-Yu , Lin Yu-Sen , Hua Chung-Hung , Lin Chen-Yuan , Su Pei-Yuan , Lai Liang-Chuan , Sher Yuh-Pyng 
Published: November 2015

AbstractMetastasis is the leading cause of death in cancer patients due to the difficulty of controlling this complex process. MicroRNAs (miRNA), endogenous noncoding short RNAs with important biological and pathological functions, may play a regulatory role during cancer metastasis, but this role has yet to be fully defined. We previously demonstrated that ADAM9 enhanced the expression of the pro-migratory protein CDCP1 to promote lung metastasis; however, the regulatory process remains unknown. Here we demonstrate that endogenous miR-218, which is abundant in normal lung tissue but suppressed in lung tumors, is regulated during the process of ADAM9-mediated CDCP1 expression. Suppression of miR-218 was associated with high migration ability in lung cancer cells. Direct interaction between miR-218 and the 3′-UTR of CDCP1 mRNAs was detected in luciferase-based transcription reporter assays. CDCP1 protein levels decreased as expression levels of miR-218 increased and increased in cells treated with miR-218 antagomirs. Induction of miR-218 inhibited tumor cell mobility, anchorage-free survival and tumor-initiating cell formation in vitro and delayed tumor metastases in mice. Our findings revealed an integrative tumor suppressor function of miR-218 in lung carcinogenesis and metastasis.
Early maternal loss affects social integration of chimpanzees throughout their lifetime
Authors: Kalcher-Sommersguter Elfriede , Preuschoft Signe , Franz-Schaider Cornelia , Hemelrijk Charlotte K. , Crailsheim Karl , Massen Jorg J. M. 
Published: November 2015

AbstractThe long-term effects of early adverse experiences on later psychosocial functioning are well described in humans, but sparsely documented for chimpanzees. In our earlier studies, we investigated the effects of maternal and social deprivation on three groups of ex-laboratory chimpanzees who experienced either an early or later onset of long-term deprivation. Here we expand our research by adding data on subjects that came from two stable zoo groups. The groups comprised of early maternally deprived wild-caught chimpanzees and non-deprived zoo-born chimpanzees. We found that compared to zoo chimpanzees, ex-laboratory chimpanzees were more restricted regarding their association partners in the newly formed groups, but not during their second year of group-life, indicating that social stability has an important influence on the toleration of association partners close-by. Social grooming activity, however, was impaired in early long-term deprived ex-laboratory chimpanzees as well as in early maternally deprived zoo chimpanzees compared to non-deprived zoo chimpanzees. Thus, we conclude that early maternal loss has lifelong effects on the social integration of chimpanzees which becomes evident in their grooming networks. Although the retrospective nature of our study prevents a clear causal explanation, our results are of importance for understanding the development of social competence in chimpanzees.
A potential mode of action for Anakinra in patients with arthrofibrosis following total knee arthroplasty
Authors: Dixon David , Coates Jonathon , del Carpio Pons Alicia , Horabin Joanna , Walker Andrew , Abdul Nicole , Kalson Nicholas S. , Brewster Nigel T. , Weir David J. , Deehan David J. , Mann Derek A. , Borthwick Lee A. 
Published: November 2015

AbstractArthrofibrosis is a fibroproliferative disease characterised by excessive deposition of extracellular matrix components intra-articularly leading to pain and restricted range of movement. Although frequently observed following total knee arthroplasty (TKA) no therapeutic options exist. A pilot study demonstrated that intra-articular injection of Anakinra, an IL-1R antagonist, improved range of movement and pain in patients with arthrofibrosis however the mechanism of action is unknown. We hypothesise that IL-1α/β will drive an inflammatory phenotype in fibroblasts isolated from the knee, therefore identifying a potential mechanism of action for Anakinra in arthrofibrosis following TKA. Fibroblasts isolated from synovial membranes and infra-patellar fat pad of patients undergoing TKA express high levels of IL-1R1. Stimulation with IL-1α/β induced a pro-inflammatory phenotype characterised by increased secretion of GMCSF, IL-6 and IL-8. No significant difference in the inflammatory response was observed between fibroblasts isolated from synovial membrane or infra-patellar fat pad. IL-1α/β treatments induced a pro-inflammatory phenotype in fibroblasts from both synovial membrane and infra-patellar fat pad and therefore Anakinra can likely have an inhibitory effect on fibroblasts present in both tissues in vivo. It is also likely that fibroblast responses in the tissues are controlled by IL-1α/β availability and not their ability to respond to it.
A mineralogical study in contrasts: highly mineralized whale rostrum and human enamel
Authors: Li Zhen , AI-Jawad Maisoon , Siddiqui Samera , Pasteris Jill D. 
Published: November 2015

AbstractThe outermost enamel of the human tooth and the rostrum of the whale Mesoplodon densirostris are two highly mineralized tissues that contain over 95 wt.% mineral, i.e., bioapatite. However, the same mineral type (carbonated hydroxylapatite) does not yield the same material properties, as revealed by Raman spectroscopy, scanning electron microscopy, electron microprobe analysis and synchrotron X-ray diffraction analysis. Overall, the outermost enamel of a tooth has more homogeneous physical and chemical features than the rostrum. Chemical comparison of rostrum and enamel shows bioapatite in the rostrum to be enriched in Na, Mg, CO3 and S, whereas the outermost enamel shows only a slightly enriched Cl concentration. Morphologically, mineral rods (at tens of μm scale), crystallites and prisms (at μm and sub-μm scale) and platelets (at tens of nm scale) all demonstrate less organized texture in the rostrum than in enamel. Such contrasts between two mineralized tissues suggest distinct pathways of biomineralization, e.g., the nature of the equilibrium between mineral and body fluid. This study illustrates the remarkable flexibility of the apatite mineral structure to match its chemical and physical properties to specific biological needs within the same animal or between species.
Near-infrared (NIR) up-conversion optogenetics
Authors: Hososhima Shoko , Yuasa Hideya , Ishizuka Toru , Hoque Mohammad Razuanul , Yamashita Takayuki , Yamanaka Akihiro , Sugano Eriko , Tomita Hiroshi , Yawo Hiromu 
Published: November 2015

AbstractNon-invasive remote control technologies designed to manipulate neural functions have been long-awaited for the comprehensive and quantitative understanding of neuronal network in the brain as well as for the therapy of neurological disorders. Recently, it has become possible for the neuronal activity to be optically manipulated using biological photo-reactive molecules such as channelrhodopsin (ChR)-2. However, ChR2 and its relatives are mostly reactive to visible light, which does not effectively penetrate through biological tissues. In contrast, near-infrared (NIR) light (650–1450 nm) penetrates deep into the tissues because biological systems are almost transparent to light within this so-called ‘imaging window’. Here we used lanthanide nanoparticles (LNPs), composed of rare-earth elements, as luminous bodies to activate ChRs since they absorb low-energy NIR light to emit high-energy visible light (up-conversion). Here, we created a new type of optogenetic system which consists of the donor LNPs and the acceptor ChRs. The NIR laser irradiation emitted visible light from LNPs, then induced the photo-reactive responses in the near-by cells that expressed ChRs. However, there remains room for large improvements in the energy efficiency of the LNP-ChR system.
Testing for Basins of Wada
Authors: Daza Alvar , Wagemakers Alexandre , Sanjuán Miguel A. F. , Yorke James A. 
Published: November 2015

AbstractNonlinear systems often give rise to fractal boundaries in phase space, hindering predictability. When a single boundary separates three or more different basins of attraction, we say that the set of basins has theWada property and initial conditions near that boundary are even more unpredictable. Many physical systems of interest with this topological property appear in the literature. However, so far the only approach to study Wada basins has been restricted to two-dimensional phase spaces. Here we report a simple algorithm whose purpose is to look for the Wada property in a given dynamical system. Another benefit of this procedure is the possibility to classify and study intermediate situations known as partially Wada boundaries.
Production of Korean Idiomatic Utterances Following Left- and Right-Hemisphere Damage: Acoustic Studies
Authors: Yang Seung-yun , Van Lancker Sidtis Diana 
Published: November 2015

Purpose
This study investigates the effects of left- and right-hemisphere damage (LHD and RHD) on the production of idiomatic or literal expressions utilizing acoustic analyses.

Method
Twenty-one native speakers of Korean with LHD or RHD and in a healthy control (HC) group produced 6 ditropically ambiguous (idiomatic or literal) sentences in 2 different speech tasks: elicitation and repetition. Utterances were analyzed using durational and fundamental-frequency (F0) measures. Listeners' goodness ratings (how well each utterance represented its category: idiomatic or literal) were correlated with acoustic measures.

Results
During the elicitation tasks, the LHD group differed significantly from the HC group in durational measures. Significant differences between the RHD and HC groups were seen in F0 measures. However, for the repetition tasks, the LHD and RHD groups produced utterances comparable to the HC group's performance. Using regression analysis, selected F0 cues were found to be significant predictors for goodness ratings by listeners.

Conclusions
Using elicitation, speakers in the LHD group were deficient in producing durational cues, whereas RHD negatively affected the production of F0 cues. Performance differed for elicitation and repetition, indicating a task effect. Listeners' goodness ratings were highly correlated with the production of certain acoustic cues. Both the acoustic and functional hypotheses of hemispheric specialization were supported for idiom production.
Comparison of Intelligibility Measures for Adults With Parkinson's Disease, Adults With Multiple Sclerosis, and Healthy Controls
Authors: Stipancic Kaila L. , Tjaden Kris , Wilding Gregory 
Published: November 2015

Purpose
This study obtained judgments of sentence intelligibility using orthographic transcription for comparison with previously reported intelligibility judgments obtained using a visual analog scale (VAS) for individuals with Parkinson's disease and multiple sclerosis and healthy controls (K. Tjaden, J. E. Sussman, & G. E. Wilding, 2014).

Method
Speakers read Harvard sentences in habitual, clear, loud, and slow conditions. Sentence stimuli were equated for peak intensity and mixed with multitalker babble. A total of 50 listeners orthographically transcribed sentences. Procedures were identical to those for a VAS reported in Tjaden, Sussman, and Wilding (2014).

Results
The percent correct scores from transcription were significantly higher in magnitude than the VAS scores. Multivariate linear modeling indicated that the pattern of findings for transcription and VAS was virtually the same with respect to differences among groups and speaking conditions. Correlation analyses further indicated a moderately strong, positive relationship between the two metrics. The majority of these correlations were significant. Last, intrajudge and interjudge listener reliability metrics for the two intelligibility tasks were comparable.

Conclusion
Results suggest that there may be instances when the less time-consuming VAS task may be a viable substitute for an orthographic transcription task when documenting intelligibility in mild dysarthria.
Leukocyte-specific protein 1 regulates T-cell migration in rheumatoid arthritis
Authors: Hwang Seong-Hye , Jung Seung-Hyun , Lee Saseong , Choi Susanna , Yoo Seung-Ah , Park Ji-Hwan , Hwang Daehee , Shim Seung Cheol , Sabbagh Laurent , Kim Ki-Jo , Park Sung Hwan , Cho Chul-Soo , Kim Bong-Sung , Leng Lin , Montgomery Ruth R. , Bucala Richard , Chung Yeun-Jun , Kim Wan-Uk 
Published: November 2015

SignificanceWe screened rheumatoid arthritis (RA)-associated copy number variations (CNVs) across the whole genome and identified significant deletion variants encompassing leukocyte-specific protein 1 (LSP1) gene. Functional assays revealed that LSP1, induced by T-cell receptor activation, negatively regulates T-cell migration. Loss ofLsp1promotes T-cell migration into antigen-instilled tissues and draining lymph nodes in mice with T-cell–dependent chronic inflammation. Moreover, patients with RA show diminished expression of LSP1 in peripheral T cells with increased migratory capacity. To our knowledge, our work is the first to demonstrate how CNVs result in immune dysfunction and a disease phenotype, highlighting the importance ofLSP1CNVs and LSP1 insufficiency in the pathogenesis of RA.
Redistribution of soil water by a saprotrophic fungus enhances carbon mineralization
Authors: Guhr Alexander , Borken Werner , Spohn Marie , Matzner Egbert 
Published: November 2015

Significance
This work shows a mechanism behind the observed higher drought resistance of soil fungi compared with bacteria. It also demonstrates the relevance of hydraulic redistribution by saprotrophic fungi for ecosystem ecology by influencing the carbon and water cycle in soils and terrestrial ecosystems under drought. Furthermore, we documented a so far underrated pathway of water in desiccated soils.
QQSorphan gene regulates carbon and nitrogen partitioning across species via NF-YC interactions
Authors: Li Ling , Zheng Wenguang , Zhu Yanbing , Ye Huaxun , Tang Buyun , Arendsee Zebulun W. , Jones Dallas , Li Ruoran , Ortiz Diego , Zhao Xuefeng , Du Chuanlong , Nettleton Dan , Scott M. Paul , Salas-Fernandez Maria G. , Yin Yanhai , Wurtele Eve Syrkin 
Published: November 2015

SignificanceEach species contains a subset of genes that are uniquely present in that species; the functions and origins of the vast majority of these “orphan genes” are not well-understood. Expression of theArabidopsis QQS(Qua-Quine Starch;At3g30720) orphan transgene increases the level of protein in soybean lines with high and low protein and acts across flowering plants to increase the protein content of maize and rice. Our results begin to dissect the mechanism of QQS functions by identifying that it binds to the conserved transcription factor nuclear factor Y, subunit C4 (NF-YC4). Increased expression ofNF-YC4inArabidopsismimics the effects of increased expression ofQQS. The ability to optimize protein productivity in plant-based foods would have far-ranging impacts on world health and sustainability.
RNA-directed DNA methylation enforces boundaries between heterochromatin and euchromatin in the maize genome
Authors: Li Qing , Gent Jonathan I. , Zynda Greg , Song Jawon , Makarevitch Irina , Hirsch Cory D. , Hirsch Candice N. , Dawe R. Kelly , Madzima Thelma F. , McGinnis Karen M. , Lisch Damon , Schmitz Robert J. , Vaughn Matthew W. , Springer Nathan M. 
Published: November 2015

Significance
RNA-directed DNA methylation (RdDM) provides a system for targeting DNA methylation to asymmetric CHH (H = A, C, or T) sites. This RdDM activity is often considered a mechanism for transcriptional silencing of transposons. However, many of the RdDM targets in the maize genome are located near genes or regulatory elements. We find that the regions of elevated CHH methylation, termed mCHH islands, are the boundaries between highly methylated (CG, CHG), silenced chromatin and more active chromatin. Analysis of RdDM mutants suggests that the function of the boundary is to promote and reinforce silencing of the transposable elements located near genes rather than to protect the euchromatic state of the genes.
Mutations in the linker domain affect phospho-STAT3 function and suggest targets for interrupting STAT3 activity
Authors: Mertens Claudia , Haripal Bhagwattie , Klinge Sebastian , Darnell James E. 
Published: November 2015

Significance
Examination of mutants in linker domain of STAT3 suggests contacts with both the DNA binding and SH2 domains that may cause structural changes and affect pSTAT3-dependent transcription, opening the possibility of new targets for drug inhibition of pSTAT3.
Conformational signaling required for synaptic plasticity by the NMDA receptor complex
Authors: Aow Jonathan , Dore Kim , Malinow Roberto 
Published: November 2015

SignificanceNeurotransmitter binding to NMDA receptors (NMDARs) produces an ion-flow-independent conformational change in its intracellular domain, as well as synaptic depression. An antibody that blocks this movement also blocks ion-flow-independent synaptic depression. The interactions of the NMDAR with signaling proteins that mediate synaptic depression, protein phosphatase 1 (PP1), and calcium-calmodulin-dependent protein kinase II (CaMKII) are monitored using fluorescence lifetime imaging microscopy (FLIM) to measure Förster resonance energy transfer (FRET). Ligand binding to the NMDAR produces changes in these interactions that require conformational movement, but not ion flow through the receptor. Our study directly measures metabotropic signaling actions by NMDARs and provides insights into the mechanisms of synaptic depression.
Center of excellence for mobile sensor data-to-knowledge (MD2K)
Authors: Kumar Santosh , Abowd Gregory D , Abraham William T , al’Absi Mustafa , Gayle Beck J , Chau Duen Horng , Condie Tyson , Conroy David E , Ertin Emre , Estrin Deborah , Ganesan Deepak , Lam Cho , Marlin Benjamin , Marsh Clay B , Murphy Susan A , Nahum-Shani Inbal , Patrick Kevin , Rehg James M , Sharmin Moushumi , Shetty Vivek , Sim Ida , Spring Bonnie , Srivastava Mani , Wetter David W 
Published: November 2015

AbstractMobile sensor data-to-knowledge (MD2K) was chosen as one of 11 Big Data Centers of Excellence by the National Institutes of Health, as part of its Big Data-to-Knowledge initiative. MD2K is developing innovative tools to streamline the collection, integration, management, visualization, analysis, and interpretation of health data generated by mobile and wearable sensors. The goal of the big data solutions being developed by MD2K is to reliably quantify physical, biological, behavioral, social, and environmental factors that contribute to health and disease risk. The research conducted by MD2K is targeted at improving health through early detection of adverse health events and by facilitating prevention. MD2K will make its tools, software, and training materials widely available and will also organize workshops and seminars to encourage their use by researchers and clinicians.