bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Studies of Treatments for Pityriasis Lichenoides
Authors: Gritiyarangsan Pimonpun , Pruenglampoo Sontaya , Ruangratanarote Pravit 
Published: April 2015

AbstractThirty patients with pityriasis lichenoides were divided into three groups. The first group was treated twice daily with applications of topical 0.02–0.1% triamcinolone cream. The second group was given the same regimen but in combination with oral tetracycline, one to two grams daily in divided doses. The third group was treated with oral psoralen and ultraviolet A (PUVA). The average durations of treatment in the three groups were 6, 7 and 2 months, respectively. The results of the treatment in the first group included two patients with complete remission, two with partial response and four with no response. In the second group, there was one patient with complete remission, eleven with partial response, and two with no response. Significant therapeutic results were observed in the third group; there were five patients with complete remission, two with partial response, and one with no response. Skin lesions of patients in the third group cleared completely or were markedly reduced with an average of 26 treatments at an average UVA dose of 68.80 joules/cm2. We therefore concluded that PUVA is more effective than the other modes of treatment for pityriasis lichenoides.
Malignant Transformation of Lymphadenosis Benigna Cutis: A Possibly Transformed Case and B‐cell Lymphoma
Authors: Nakayama Hidetoshi , Mihara Motoyuki , Shimao Shuhei 
Published: April 2015

AbstractThis study presents a patient who had had a 10 year history of lymphadenosis benigna cutis (LABC) before malignant lymphoma of the skin subsequently developed. During the LABC stage, the lesion showed several foci of cavernous hemangioma‐like nodules which disappeared spontaneously but reccurred repeatedly. These cutaneous nodules were finally diagnosed as malignant lymphoma of the skin with a histology of diffuse, mixed small and large cell type according to the classification of the Working Formulation. Among the subpopulations of tumor cells, B‐cells were highly predominant. It is suggested that LABC may have the potential to transform into malignant lymphoma (probably B‐cell lymphoma).
Cutaneous Metastasis of Invasive Lobular Carcinoma of the Breast
Authors: Aizawa Harumi , Aiba Setsuya , Takahashi Masaaki , Tagami Hachiro 
Published: April 2015

AbstractAs far as we know there has been no specified report of cutaneous metastasis from invasive lobular carcinoma of the breast. This type of carcinoma constitutes a minor histological group in all breast cancers, especially in Japan. We describe two such cases whose histologic features were conspicuous; numerous dissociated round cells that were arranged in linear arrays. It resembled the histology of cancer en cuirasse to some extent, but the density and variety of tumor cell nests were much richer. The arrangement of the tumor cells as well as their cytology was identical to that of the primary lesions.
Epidermolytic Hyperkeratosis Associated with a Dilated Pore
Authors: Tokura Yoshiki , Takigawa Masahiro , Yamada Mizuho 
Published: April 2015

AbstractA case of a dilated pore containing a histologic focus of epidermolytic hyperkeratosis was reported. Our case widened the spectrum to include the association of epidermolytic hyperkeratosis with acquired solitary skin lesions.
Lanthanum‐induced Alterations in the Distribution of Actin Filaments in Human Keratinocytes
Authors: Kitano Yukio 
Published: April 2015

AbstractThe effects of La3+ on the distribution and organization of actin in human keratinocytes were examined with rhodamine‐phalloidin staining. At 0.2 and 0.02 mM concentrations, La3+ induced redistribution and organization of actin. Thick bundles of actin filaments appeared, as did fine ripple‐like short bundles and polygonal structures. Thick, ribbon‐like assemblages of actin were crescent‐shaped or semi‐circular. These changes were quite similar to those induced by TPA. The effects of La3+ were discussed with reference to Ca2+.
Qualitative and Quantitative Studies of Paget Cells in the Horny Layer
Authors: Palungwachira Piti , Kurumatani Mineko , Miyashita Mitsuo , Baba Shunichi , Suzuki Hiroyuki , Morioka Sadao 
Published: April 2015

AbstractOne case of extramammary Paget's disease was studied by ultrastructural and morphometrical methods to clarify whether or not Paget cells change qualitatively or quantitatively in passing through the epidermis. Paget cells in living layers were observed as large cells with abundant clear cytoplasm, large nuclei, and prominent nucleoli. The cytoplasm was enriched with well developed rough‐surfaced endoplasmic reticulum, mitochondria, free ribosomes, and glycogen particles. In contrast, filamentous structures were not developed. Paget cells are connected to each other and adjacent keratinocytes by small desmosomes.In contrast, in the horny layer, Paget cells contained shrunken, flattened nuclei. Cell organelles had degenerated and large lipid droplets were present in the cytoplasm. As disruption of the desmosomes took place, intercellular spaces between Paget cells and keratinocytes widened. Based on the morphometrical analysis by a sonic digitizer computer system, there were statistically significant decreases in areas and diameters of Paget cells between the horny layer and the living cell layers, including the basal, spinous and granular layers.In conclusion, it is suggested that Paget cells change qualitatively and quantitatively after they move into the horny layer from the living layers.
Anti‐keratin Monoclonal Antibody against Basal Cell Epithelioma Keratin: BKN‐1
Authors: Shimizu Naoya , Ito Masaaki , Tazawa Toshio , Katsuumi Kaoru , Sato Yoshio 
Published: April 2015

AbstractA BALB/c mouse was immunized with fibrous proteins (FP) extracted from basal cell epithelioma (BCE) and anti‐keratin monoclonal antibodies were produced by a hybridoma technique with a mouse myeloma cell line. One monoclonal antibody was designated as BKN‐1.Immunohistochemically, in 17 cases of BCE, the cytoplasm of all the tumor cells was always stained by BKN‐1. In the normal human skin tissue, BKN‐1 specifically reacted with the basal cells in the epidermis and in the infundibular epithelium of the hair follicle, the entire follicular cells below the isthmus portion in the anagen hair follicle, the peripheral cells of sebaceous glands, and sweat gland cells. The reaction of BKN‐1 was immunoelectron microscopically located on the tonofilaments in the cytoplasm. By immunoblot analysis, BKN‐1 stained either a band of 56–56.5K in FP from BCE or several bands in normal epidermal FP.These results suggest that the keratin expression of BCE may resemble that of the follicular epithelium below the isthmus portion.
Light and Electron Microscopic Immunohistochemistry of Solar Elastosis: A Study with Cutis Rhomboidalis Nuchae
Authors: Matsuta Mayumi , Izaki Seiichi , Ide Chizuka , Izaki Masakatsu 
Published: April 2015

AbstractBiopsied skin specimens from 10 males with cutis rhomboidalis nuchae, 50–83 years in age, as well as age‐matched non‐sun‐exposed skin and young normal skin specimens were subjected to a light and electron microscopic immunohistochemical study using anti‐elastin polyclonal antibody and anti‐microfibril HB‐8 monoclonal antibody. Conventional histochemical and electron microscopic techniques were also used.The most severely degenerated elastotic materials had developed in the upper dermis of the cutis rhomboidalis nuchae cases. Light microscopic histochemistry with Elastica van Gieson and Azan staining revealed elastotic materials with elastic fiber characteristics. Electron microscopic observation showed that the elastotic materials consisted of disintegrated and dispersed tannic‐acid staining elastin, fine granular materials, and filamentous substances. Light microscopic immunohistochemistry showed that the upper dermis from cutis rhomboidalis nuchae samples was reactive to anti‐elastin but not to anti‐microfibril HB‐8 antibody. Electron microscopic immunohistochemistry further revealed that reticularly disintegrated substances in the elastotic materials retained their reactivity to anti‐elastin, but did not show any reactivity to anti‐microfibril HB‐8 monoclonal antibody. These findings show that these elastotic materials were derived from elastic fibers and partially retained their antigenicity for elastin, but not for microfibrils.
A Case of Donovanosis in Japan
Authors: Fujiwara Sakuhei , Honda Tomohito , Shinkai Hiroshi , Takayasu Susumu , Chen Shin‐Zon , Mori Hiroyuki , Sehgal Virender Nath 
Published: April 2015

AbstractA 21‐year‐old woman had large, painless, genital ulcers during pregnancy. The biopsy specimen showed a dense infiltration of mononuclear cells throughout the whole dermis. The histological sections with slow Giemsa staining revealed purplish‐blue colored intracytoplasmic Donovan bodies. The genital lesions rapidly improved with antibiotics. She and her husband had never traveled in endemic areas.
Hemorrhage into a Plexiform Neurofibroma Induced by Trauma: A Rare Complication of von Recklinghausen's Disease
Authors: Sawada Yukimasa 
Published: April 2015

AbstractA case of hemorrhage into a plexiform neurofibroma of a 53‐year‐old woman is described. Immediately after trauma to the plexiform neurofibroma on her scalp, she noticed severe headaches and sudden enlargement of the tumor. The tumor continued to enlarge slowly. Her headaches also continued until tumor excision. The specimen taken during surgery surrounded a round cavity about 7 cm in diameter containing coagulated blood. Numerous, old, perivascular hemorrhages around many dilated vessels with extremely thin walls were revealed by histological examination.
The Major Histocompatibility Complex and the Fugu Aspect of Immunity
Authors: Klein Jan 
Published: April 2015

AbstractThe major histocompatibility complex (Mhc) is a set of genes that codes for plasma‐membrane associated proteins, which are primarily expressed on the cells and tissues of the immune system. The proteins serve as cell markers during the initiation phase of immune response. T lymphocytes recognize Mhc molecules with their receptors when they recognize a foreign antigen on the surface of an antigen presenting cell. The functional Mhc genes are highly polymorphic. One consequence of this polymorphism is the existence of nonresponders to foreign antigens. Certain combinations of self Mhc molecules and nonself antigens fail to stimulate an immune response. A possible explanation for this nonresponsiveness is the need to make an individual unresponsive to (tolerant of) its own proteins. Tolerance leads to the inactivation of certain T cell clones; thus, if the same clones also happen to recognize a particular foreign antigen, the individual becomes unresponsive to this antigen as well. It then contains a blind spot in its repertoire.
Clinical Characteristics of Pigmented Macules on Palms and/or Soles Appearing after Middle Age in Japan
Authors: Narisawa Yutaka , Kohda Hiromu 
Published: April 2015

AbstractWe have examined the clinical characteristics of pigmented macules on palms and/or soles. A total of 238 inpatients and 111 healthy persons over 40 years old in Japan were included in our analysis. We found: (a) that such pigmented macules may occur not only on healthy persons but also on patients who suffer from various internal disorders, (b) that, in both males and females, the incidence of pigmented macules is relatively high in malignancy patients in comparison with non‐malignancy and control individuals, (c) that the incidence is higher in males than in females, (d) that, in both males and females, there was a tendency toward distribution on both palms and soles in malignancy patients in comparison with the non‐malignancy and control individuals (p<0.01), and (e) that, among cases with pigmented macules, the number of pigmented macules is empirically greater in inpatients (the malignancy and non‐malignancy groups) than in the controls.
The Mechanisms of Histological Incontinence of Pigment: Light and Electron Microscopic Studies of Lesions and Patch Test Sites in Female Facial Melanosis and DNCB Reactions in Guinea Pigs
Authors: Onoda Susumu 
Published: April 2015

AbstractHistological incontinence of pigment (HIP) was studied using light and electron microscopy in a pigmented lesion from a female facial melanosis (FFM) patient and in another lesion produced by isoeugenol when the patient was patch tested. The following sequence of events is thought to underlie HIP. Phagocytes invade the epidermis and phagocytize melanosomes either in keratinocytes or in cytoid bodies, which are degenerated keratinocytes. These phagocytes then return to the dermis through gaps in the basal lamina.In brown guinea pigs sensitized to DNCB, 0.02% DNCB in acetone was applied repeatedly to the same area of the abdomen once per week for six weeks. HIP was observed in 28% of the animals following the fifth application of DNCB. In contrast to the HIP process observed in the patient, phagocytes in brown guinea pigs phagocytized free melanosomes. In irritant reactions to DNCB in brown guinea pigs, only concentrations which produced epidermal necrosis induced HIP.
Ultrastructural Sites of Blister Formation in Dermatitis Herpetiformis: Report of a Case and Retrospective Electron Microscopy Using Routine Histologic Preparations
Authors: Horiguchi Yuji , Danno Kiichiro , Toda Ken‐ichi , Takada Kazuyo , Komura Jinro , Horio Takeshi , Imamura Sadao 
Published: April 2015

AbstractElectron microscopy of the skin lesions of a patient with the classical type of dermatitis herpetiformis (DH), showing granular IgA deposits beneath the epidermis and gluten‐sensitive enteropathy, revealed that the initial changes of the vesicular or blister formation are neutrophilic invasion above the basal lamina and consequent degeneration of the basal cells. Retrospective electron microscopic studies using the refixation‐reembedding method performed on four previous cases of classical DH revealed the coexistence of degeneration of the basal cells and dermal vacuolization in two cases, blister formation above the basal lamina in one case, and blister formation in the uppermost dermis with basal lamina remaining along the blister roof in one case. The infiltrating inflammatory cells in the skin of classical type of DH seem to injure the epidermal‐dermal junction, including the basal cells and the uppermost dermis, to initiate the blister or vesicular formation.
Cutaneous Infection Caused by Fusarium solani and Fusarium oxysporum
Authors: Uchiyama Noriko , Shindo Yasuko , Mikoshiba Hajime , Saito Hideki 
Published: April 2015

AbstractA case of cutaneous infection by Fusarium solani and Fusarium oxysporum on the middle finger of the right hand of a 58‐year‐old farmer is reported. On the extensor aspect of the distal phalanx of the middle finger, there was a pale reddish, edematous lesion with some vesicles, pustules, and an ulcer. Scrapings from the lesion showed fungal elements on direct microscopic examination, and Fusarium solani and Fusarium oxysporum were identified on slide culture. Hyperthermia treatment with a chemical pocket warmer led to conspicuous healing of the lesion.
Microsporum ferrugineum Infection with Unusual Clinical Features
Authors: Shoji Akinobu , Kitajima Jun'ichi , Hamada Toshio 
Published: April 2015

AbstractUnusual skin manifestations of a dermatophyte infection in a 30‐year‐old female were reported. These included a remarkable peripheral hyperkeratosis with central yellowish pus. The pinkish papillomatous lesions were covered with thick crusts. They gave off a foul smell. Abundant fungus elements were present in the crusts and keratotic layer. The trichophytin skin test was positive. Immunologic test results revealed that the patient had hypergammaglobulinemia with hypoalbuminemia. However, she had no edema or immunologic abnormalities. The skin lesions were successfully treated with topical miconazole cream, oral griseofulvin, and topical sulconazole cream. The etiologic agent was identified as Microsporum ferrugineum Ota 1922. It seemed to us that the unusual skin manifestation were induced by her immunological response to fungal and bacterial infection due to a long history of Candida granuloma.
Systemic Mastocytosis
Authors: Lo Wen‐Lin , Wong Chu‐Kwan 
Published: April 2015

AbstractA 74‐year‐old Chinese male has had diffuse asymptomatic thickening, wrinkling, and mild hyperpigmentation of the skin with prominent skin markings and a leathery appearance over the neck, abdomen, flanks, and flexural area for forty years. Darter's sign was positive. Skin biopsy showed a dense band‐like infiltration of mast cells into the upper dermis. Mast cell infiltration into the bone marrow and liver was also proved histologically. Electron microscopic studies of the skin and liver specimens were performed. Related aspects of diffuse and systemic mastocytosis are discussed. To our knowledge, this is the first reported Chinese case of diffuse and systemic mastocytosis in English literature. A review of the literature on systemic mastocytosis is also included.
Erythema Dyschromicum Perstans/Ashy Dermatosis
Authors: Phay Ken Lin , Goh Chee Leok 
Published: April 2015

AbstractEight patients with erythema dyschromicum perstans (ashy dermatosis) are described. The characteristic ashy macules occurred mainly on the trunk and limbs. The condition did not appear to be associated with infection or drug ingestion. Seven patients had preceding erythema before onset of ashy macules; three had urticarial eruptions associated with the early erythema. Biopsy consistently showed mild, superficial, perivascular mononuclear cell infiltration and abundant melanophages in all the patients. In five, vascuolar degeneration of the basal epidermal cells was evident. There was no evidence to suggest that the condition was associated with lichen planus. The dermatosis appeared to run a prolonged course.
Corticosteroid‐modified Tinea Faciei Simulating Rosacea
Authors: Dekio Satoshi , Imaoka Chiharu , Jidoi Joji 
Published: April 2015

AbstractTinea faciei simulating rosacea which developed after topical application of a corticosteroid ointment is described. Mycological examinations revealed that the causative organism was Trichophyton mentagrophytes. The tinea was cured by topical application of a cream containing tolciclate.
Dermatomal Pruritus of the Upper Limb: A Manifestation of Nerve Root Compression due to Degenerative Spine Disease?
Authors: Grob Jean‐Jacques , Bonerandi Jean‐Jacques 
Published: April 2015

AbstractA case of pruritus and lichenification localized to a band area on the arm is reported. It is interpreted as a dermatomal pruritus due to C5‐C6 radiculitis caused by degenerative spine disease. This unusual case is compared to an other entity known as branchioradial pruritus.
Immunohistochemical Characterization of Spitz's Nevus: Differentiation from Common Melanocytic Nevus, Dysplastic Melanocytic Nevus and Malignant Melanoma
Authors: Takahashi Hiroyuki , Maeda Kaori , Maeda Kazuo , Akutsu Yutaka , Horikoshi Takashi , Jimbow Kowichi 
Published: April 2015

AbstractRecently it has been reported that Spitz's nevus possesses a deranged melanogenesis with formation of the spherical melanosomes also seen in superficial spreading melanoma (SSM) and dysplastic melanocytic nevus (DMN). To characterize the nature of Spitz's nevus, immunohistochemical studies were carried out in 9 cases of this condition using monoclonal antibodies (MoAbs) which identify (a) human melanosome‐associated antigen (HMSA‐1 and HMSA‐2), (b) S‐100 protein (α and β subunits), (c) Leu‐7 (HNK‐1), (d) β2 microglobulin (B2MG), and (e) neuron specific enolase (NSE). In contrast to SSM and DMN, none of the 9 cases showed any significant reactivity with MoAbs HMSA‐1 and HMSA‐2. Similar to cutaneous malignant melanoma (CMM) and DMN, and unlike common melanocytic nevus (CMN), anti‐S‐100 protein α subunit MoAb reacted from moderately to intensely with Spitz's nevus, and anti‐S‐100 protein β subunit MoAb reacted weakly. Anti‐B2MG MoAb was reactive with 8 of 9 cases. Only one case showed cytoplasmic reactivity to anti‐Leu‐7 MoAb. Polyclonal NSE was found in 7 cases at varying intensities. Our immunohistochemical study indicates the distinct, benign neoplastic nature of Spitz's nevus which has immunoreactivities differing from those of CMM, DMN and CMN.
Superoxide Dismutase in Epidermis: Its Relation to Keratinocyte Proliferation
Authors: Ohkuma Noritaka , Kajita Satoshi , Iizuka Hajime 
Published: April 2015

AbstractDecreased superoxide dismutase (SOD) activity has been reported in various hyperproliferative tissues. In order to elucidate the significance of SOD activity in keratinocyte proliferation, we measured the SOD activity in several pathologic epidermal conditions. The SOD activity was significantly decreased in psoriatic hyperproliferative epidermis as well as in basal cell epithelioma, squamous cell carcinoma and seborrheic keratosis.Following tape stripping in vivo, pig epidermis revealed a marked increase in thymidine uptake and mitotic counts. The peak of the thymidine uptake was observed at 24 h, which was followed by the increased mitotic counts (peak at 48 h). The pig skin revealed a decreased epidermal SOD activity following the tape stripping. A decline of SOD activity was observed within 7 days after the tape stripping and about a 50% decrease was observed at 48 h. Epidermis is known to contain both Cu, Zn‐SOD and Mn‐SOD activities; the decreased SOD activity after tape stripping was mainly due to decreased Cu, Zn‐SOD activity without any significant alteration of Mn‐SOD activity.Following UVB irradiation (2MED) in vivo, pig epidermis revealed decreased thymidine incorporation as well as decreased mitotic counts. Epidermal SOD activity, however, was not altered by the UVBirradiation. Pig epidermis is also known to indicate decreased cell proliferative activity in the presence of various chemicals in vitro. These modalities, which include retinoid (Ro 10‐1670), colchicine and protein synthesis inhibitors (puromycin, actinomycin D, cycloheximide), had no effect on the epidermal SOD activity.Our results indicate that, although the decreased SOD activity appears to be comcomitant with keratinocyte hyperproliferation, no alteration of SOD activity is observed in hypoproliferative epidermis. The significance of the altered SOD activity in the regulatory mechanism of keratinocyte proliferation remains unknown at present.
Two Unusual Cases of Deep Lymphangioma
Authors: Kikuchi Ichiro , Takasaki Naoya 
Published: April 2015

AbstractTwo cases of deep lymphangioma which were difficult to diagnose at first examination were reported. One case was battered‐child‐like, while the other presented a progressive cystic course. The onset in both cases was at birth, and both of them presented purpura during their courses. The diagnosis and treatment of these cases were difficult and required special consideration.