bluuebunny/crossref_metadata_2025_split huggingface dataset:
PRC2 inhibition counteracts the culture-associated loss of engraftment potential of human cord blood-derived hematopoietic stem and progenitor cells
Authors: Varagnolo Linda , Lin Qiong , Obier Nadine , Plass Christoph , Dietl Johannes , Zenke Martin , Claus Rainer , Müller Albrecht M. 
Published: July 2015

AbstractCord blood hematopoietic stem cells (CB-HSCs) are an outstanding source for transplantation approaches. However, the amount of cells per donor is limited and culture expansion of CB-HSCs is accompanied by a loss of engraftment potential. In order to analyze the molecular mechanisms leading to this impaired potential we profiled global and local epigenotypes during the expansion of human CB hematopoietic stem and progenitor cells (HPSCs). Human CB-derived CD34+ cells were cultured in serum-free medium together with SCF, TPO, FGF, with or without Igfbp2 and Angptl5 (STF/STFIA cocktails). As compared to the STF cocktail, the STFIA cocktail maintains in vivo repopulation capacity of cultured CD34+ cells. Upon expansion, CD34+ cells genome-wide remodel their epigenotype and depending on the cytokine cocktail, cells show different H3K4me3 and H3K27me3 levels. Expanding cells without Igfbp2 and Angptl5 leads to higher global H3K27me3 levels. ChIPseq analyses reveal a cytokine cocktail-dependent redistribution of H3K27me3 profiles. Inhibition of the PRC2 component EZH2 counteracts the culture-associated loss of NOD scid gamma (NSG) engraftment potential. Collectively, our data reveal chromatin dynamics that underlie the culture-associated loss of engraftment potential. We identify PRC2 component EZH2 as being involved in the loss of engraftment potential during the in vitro expansion of HPSCs.
S-acylation of the Insulin-Responsive Aminopeptidase (IRAP): Quantitative analysis and Identification of Modified Cysteines
Authors: Werno Martin W. , Chamberlain Luke H. 
Published: July 2015

AbstractThe insulin-responsive aminopeptidase (IRAP) was recently identified as an S-acylated protein in adipocytes and other tissues. However, there is currently no information on the extent of S-acylation of this protein, the residues that are modified, or the effects of S-acylation on IRAP localisation. In this study, we employ a semi-quantitative acyl-RAC technique to show that approximately 60% of IRAP is S-acylated in 3T3-L1 adipocytes. In contrast, S-acylation of GLUT4, a glucose transporter that extensively co-localises with IRAP, was approximately five-fold lower. Site-directed mutagenesis was employed to map the sites of S-acylation on IRAP to two cysteine residues, one of which is predicted to lie in the cytoplasmic side of the single transmembrane domain and the other which is just upstream of this transmembrane domain; our results suggest that these cysteines may be modified in a mutually-exclusive manner. Although S-acylation regulates the intracellular trafficking of several transmembrane proteins, we did not detect any effects of mutating the modified cysteines on the plasma membrane localisation of IRAP in HEK293T cells, suggesting that S-acylation is not essential for the movement of IRAP through the secretory pathway.
The Effect of Chronological Age on the Acceptance of Internet-Based Hearing Health Care
Authors: Moore Ashley N. , Rothpletz Ann M. , Preminger Jill E. 
Published: July 2015

Purpose
The purpose of this article is to offer design considerations in developing Internet-based hearing health care for older adults by analyzing and discussing the relationship between chronological age, computer skills, and the acceptance of Internet-based hearing health care.

Method

This article reports baseline data from a training study measuring the acceptance of Internet-based hearing health care. Participants (
n
= 26; 20 men, 6 women) were aged 55–95 years. All passed a cognitive screen and failed a hearing screen. Participants completed the Patient-Technology Acceptance questionnaire (Or, 2008). Computer literacy was measured using the Northstar Digital Literacy Assessment (Cytron-Hysom, Hadley, Vanek, Graif, & Asp, 2012).

Results
Computer literacy was negatively correlated with increasing age. Additional negative relationships were seen between computer literacy and computer anxiety and between computer literacy and computer self-efficacy. Finally, there was a negative relationship between computer self-efficacy and computer anxiety.

Conclusion
These results suggest computer literacy is lower in adults of advanced age than in those who are a few years younger. Indirect relationships were observed between age and computer self-efficacy and between age and computer anxiety. Consideration should be given to addressing discrepancies in self-efficacy and computer literacy in older adults to increase the likelihood of acceptance of Internet-based hearing health care.
The Application of Intervention Coding Methodology to Describe the Tinnitus E-Programme, an Internet-Delivered Self-Help Intervention for Tinnitus
Authors: Greenwell Kate , Featherstone Debbie , Hoare Derek J. 
Published: July 2015

Purpose
This article describes the Tinnitus E-Programme, a previously untested Internet-delivered self-help intervention for tinnitus.

Method
Intervention coding methodology was applied to describe the intervention components, techniques, and mode of delivery.

Results
The intervention consists of 5 self-management intervention components, 5 behavior change techniques, and 3 modes of Internet delivery, which aim to promote relaxation behavior and reduce tinnitus distress.

Conclusions
The intervention coding provided a reliable method for reporting Internet-delivered self-help interventions. It will be used to facilitate our understanding of the intervention's potential mechanisms of change and will guide future evaluation work.
Can a Remotely Delivered Auditory Training Program Improve Speech-in-Noise Understanding?
Authors: Abrams Harvey B. , Bock Kirsten , Irey Ryan L. 
Published: July 2015

Purpose
The aims of this study were to determine if a remotely delivered, Internet-based auditory training (AT) program improved speech-in-noise understanding and if the number of hours spent engaged in the program influenced postintervention speech-in-noise understanding.

Method
Twenty-nine first-time hearing aid users were randomized into an AT group (hearing aids + 3 week remotely delivered, Internet-based auditory training program) or a control group (hearing aids alone). The Hearing in Noise Test (Nilsson, Soli, & Sullivan, 1994) and the Words-in-Noise test (Wilson, 2003) were administered to both groups at baseline + 1 week and immediately at the completion of the 3 weeks of auditory training.

Results
Speech-in-noise understanding improved for both groups at the completion of the study; however, there was not a statistically significant difference in postintervention improvement between the AT and control groups. Although the number of hours the participants engaged in the AT program was far fewer than prescribed, time on task influenced the postintervention Words-in-Noise but not Hearing in Noise Test scores.

Conclusion
Although remotely delivered, Internet-based AT programs represent an attractive alternative to resource-intensive, clinic-based interventions, their demonstrated efficacy continues to remain a challenge due in part to issues associated with compliance.
Bridging the Gap Between Hearing Screening and Successful Rehabilitation: Research Protocol of a Randomized Controlled Trial of Motivational Interviewing via Internet
Authors: Mikaela Weineland Sandra , Andersson Gerhard , Lunner Thomas , Carlbring Per , Hesser Hugo , Ingo Elisabeth , Molander Peter , Nordqvist Peter , Laplante-Lévesque Ariane 
Published: July 2015

Purpose
Studies point to low help-seeking after a failed hearing screening. This research forum article presents the research protocol for a randomized controlled trial of motivational interviewing via the Internet to promote help-seeking in people who have failed an online hearing screening.

Method
Adults who fail a Swedish online hearing screening, including a speech-in-noise recognition test, will be randomized to either an intervention group (participating in motivational interviewing) or an active control group (reading a book on history of hearing aids). Both of the conditions will be delivered via the Internet. The primary outcome is experience with seeking health care and using hearing aids 9 months after the intervention. Secondary outcomes are changes in before and after measures of self-reported hearing difficulties, anxiety, depression, and quality of life. Stages of change and self-efficacy in hearing help-seeking are measured immediately after intervention and at a 9-month follow-up for the purpose of mediation analysis.

Results
The results of this randomized controlled trial may help bridge the gap between hearing screening and successful hearing rehabilitation.

Conclusion
Although no large instantaneous benefits are expected, a slow change toward healthy behaviors—seeking health care and using hearing aids—would shed light on how to use the Internet to assist people with hearing impairment.
Internet-Based Acceptance and Commitment Therapy for Psychological Distress Experienced by People With Hearing Problems: Study Protocol for a Randomized Controlled Trial
Authors: Molander Peter , Hesser Hugo , Weineland Sandra , Bergwall Kajsa , Buck Sonia , Hansson-Malmlöf Johan , Lantz Henning , Lunner Thomas , Andersson Gerhard 
Published: July 2015

Purpose
Psychological distress and psychiatric symptoms are prevalent among people with hearing loss or other audiological conditions, but psychological interventions for these groups are rare. This article describes the study protocol for a randomized controlled trial for evaluating the effect of a psychological treatment delivered over the Internet for individuals with hearing problems and concurrent psychological distress.

Method
Participants who are significantly distressed will be randomized to either an 8-week Internet-delivered acceptance-based cognitive behavioral therapy (i.e., acceptance and commitment therapy [ACT]), or wait-list control. We aim to include measures of distress associated with hearing difficulties, anxiety, and depression. In addition, we aim to measure acceptance associated with hearing difficulties as well as quality of life.

Conclusion
The results of the trial may further our understanding of how to best treat people who present problems with both psychological distress and hearing in using the Internet.
Implementing Internet-Based Aural Rehabilitation in a General Clinical Practice
Authors: Malmberg Milijana , Lunner Thomas , Kähäri Kim , Jansson Gunilla , Andersson Gerhard 
Published: July 2015

Purpose
The purpose of this paper is to share the lessons that were learned about the process of implementing an Internet-based, randomized controlled trial (RCT) in general clinical practice (GCP) and to address some of the advantages of using the Internet as a tool to implement a RCT in GCP. The RCT implemented focused on investigating Internet-based aural rehabilitation (AR) in addition to hearing aid (HA)-fitting supplemented with telephone support, and it was applied in a clinical setting. The results of this RCT and the questionnaires chosen will be presented in an article elsewhere.

Procedure

Here, the procedure of the implemented trial is presented, and the implementation challenges are presented and discussed. Specifically, we describe the trial
research question
,
recruitment
strategy, patient
eligibility criteria
, the
questionnaires
,
clinician participation
,
funding
and
time
(for the clinicians), and
risks
and
benefits
(for the participants).

Discussion
The trial implementation showed that AR in addition to HA-fitting can be carried out in GCP using the Internet. Using an Internet-based RCT overcomes some of the challenges of implementing a trial in GCP.
Frequency Importance Functions in Quiet and Noise for Adults With Cochlear Implants
Authors: Sladen Douglas P. , Ricketts Todd. A. 
Published: July 2015

PurposeSeveral studies have been devoted to understanding the frequency information available to adult users of cochlear implants when listening in quiet. The objective of this study was to construct frequency importance functions for a group of adults with cochlear implants and a group of adults with normal hearing both in quiet and in a +10 dB signal-to-noise ratio.MethodTwo groups of adults, 1 with cochlear implants and 1 with normal hearing, were asked to identify nonsense syllables in quiet and in the presence of 6-talker babble while “holes” were systematically created in the speech spectrum. Frequency importance functions were constructed.ResultsResults showed that adults with normal hearing placed greater weight on bands 1, 3, and 4 than on bands 2, 5, and 6, whereas adults with cochlear implants placed equal weight on all bands. The frequency importance functions for each group did not differ between listening in quiet and listening in noise.ConclusionsAdults with cochlear implants assign perceptual weight toward different frequency bands, though the weight assignment does not differ between quiet and noisy conditions. Generalizing these results to the broader population of adults with implants is constrained by a small sample size.
Cavity‐backed on‐body antenna for customised hearing instrument applications
Authors: Ruaro A. , Thaysen J. , Jakobsen K.B. 
Published: July 2015

A novel on‐body antenna targeted for 2.45 GHz customised in‐the‐ear hearing instrument applications is designed, implemented, and measured. The antenna is designed to use the maximum volume available in the user's ear canal and to provide isolation from the user's body. The polarisation is primarily normal to the surface of the head to ensure the best on‐body path gain. Efficiency and S‐parameters from measurements in free space and on a specific anthropomorphic mannequin head endowed with ears are presented.
Ultrasonography as a Guiding Method in Breast Micro-Calcification Vacuum-Assisted Biopsies
Authors: Keränen A. K. , Haapea M. , Rissanen T. 
Published: July 2015

Abstract
Purpose: To assess the visibility of breast micro-calcifications using ultrasonography (US) and the accuracy and clinical usefulness of vacuum-assisted biopsy (VAB) using US guidance (USVAB) as compared to stereotactic guidance (SVAB).
Materials and Methods: The study material comprised 158 retrospectively reviewed micro-calcification cases examined with US before VAB. The pre-biopsy US positivity frequency distributions were calculated, and the sensitivity, specificity and accuracy of VAB determined by comparing VAB histology with the surgical pathology or a 12-month follow-up.
Results: 158 US examinations yielded 80 positive and 78 negative results. US positivity correlated to a large size and a suspicious BI-RADS category of the calcifications. USVAB was performed in 49 cases with 61 % malignant, 12 % high-risk and 27 % benign results. The percentages for the 109 SVAB cases were 40 %, 28 % and 32 %, respectively. Specimen radiography demonstrated calcifications in 48 of the 49 (98 %) USVAB cases and in 107 of the 109 (98 %) SVAB cases. The overall accuracy of VAB was 94 % (USVAB 98 %, SVAB 94 %), the sensitivity was 88 % (USVAB 97 %, SVAB 83 %), and the specificity was 100 %. The higher sensitivity of USVAB was due to an accumulation of atypical hyperplasia diagnoses in the SVAB group. The final diagnosis was invasive ductal carcinoma in 21 US-positive and in 4 US-negative cases.
Conclusion: Approximately 50 % of mammographically detected micro-calcifications could be detected with ultrasonography. US was found to be a valuable alternative guidance method for vacuum-assisted biopsy of micro-calcifications with a technical success rate and diagnostic accuracy well comparable to the stereotactic method.
Memory formation and retrieval of neuronal silencing in the auditory cortex
Authors: Nomura Hiroshi , Hara Kojiro , Abe Reimi , Hitora-Imamura Natsuko , Nakayama Ryota , Sasaki Takuya , Matsuki Norio , Ikegaya Yuji 
Published: July 2015

Significance
Cortical neurons exhibit spontaneous activity without explicit external stimuli. Sensory stimuli not only activate specific populations of those neurons but can also silence other populations. However, it remains unclear whether neuronal silencing per se leads to memory formation and behavioral expression. Here, we used optogenetic silencing of a neuronal ensemble in the auditory cortex and found that mice exhibited a fear response to silencing that had been previously paired with footshock, and could find a reward based on the presence or absence of the silencing. These findings indicate that neuronal silencing per se, which is originally believed to be involved in neuronal modulation, is enough to elicit a cognitive behavior.
Near-infrared fluorescence molecular imaging of amyloid beta species and monitoring therapy in animal models of Alzheimer’s disease
Authors: Zhang Xueli , Tian Yanli , Zhang Can , Tian Xiaoyu , Ross Alana W. , Moir Robert D. , Sun Hongbin , Tanzi Rudolph E. , Moore Anna , Ran Chongzhao 
Published: July 2015

Significance
Drug development for Alzheimer’s disease (AD) has been largely unsuccessful to date. Although numerous agents are reportedly effective in vitro, only an inadequate number of them have been tested in vivo, partially because of the lack of reliable and cost-efficient imaging methods to monitor their in vivo therapeutic effectiveness. Several amyloid beta (Aβ)-specific PET tracers have been used for clinical studies. However, their application for monitoring drug treatment in small animals is limited. Near-infrared fluorescence (NIRF) molecular imaging is a cheap, easy-to-use, and widely available technology for small animal studies. In this report we demonstrate, for the first time to our knowledge, that NIRF imaging can be used to monitor the loading changes of Aβs in an AD mouse model.
Addendum to ‘velocity-information based force-term estimation of dielectric barrier discharge plasma actuators’
Authors: Kriegseis J , Maden I , Schwarz C , Tropea C , Grundmann S 
Published: July 2015

Abstract
In a recent publication (Kriegseis et al 2013 J. Phys. D: Appl. Phys. 46 055202) we contrasted the variety of published strategies to estimate magnitude and distribution of the body force produced by dielectric barrier discharge plasma actuators. All approaches were applied to an identical set of velocity data, which was experimentally obtained by means of particle image velocimetry. Particularly, the determined force distributions of the above paper turned out to be beneficial for subsequent efforts to derive empirical plasma-actuator models. The purpose of this addendum, therefore, is to provide both the velocity data and the determined force distributions as discussed in the original publication. As such, a comparison of different empirical models based on identical velocity data becomes possible. In addition, numericists can directly implement the published force fields in so as to perform computational studies of discharge-based flow control.
Identifying novel associations between small molecules and miRNAs based on integrated molecular networks
Authors: Lv Yingli , Wang Shuyuan , Meng Fanlin , Yang Lei , Wang Zhifeng , Wang Jing , Chen Xiaowen , Jiang Wei , Li Yixue , Li Xia 
Published: July 2015

AbstractMotivation: miRNAs play crucial roles in human diseases and newly discovered could be targeted by small molecule (SM) drug compounds. Thus, the identification of small molecule drug compounds (SM) that target dysregulated miRNAs in cancers will provide new insight into cancer biology and accelerate drug discovery for cancer therapy.Results: In this study, we aimed to develop a novel computational method to comprehensively identify associations between SMs and miRNAs. To this end, exploiting multiple molecular interaction databases, we first established an integrated SM-miRNA association network based on 690 561 SM to SM interactions, 291 600 miRNA to miRNA associations, as well as 664 known SM to miRNA targeting pairs. Then, by performing Random Walk with Restart algorithm on the integrated network, we prioritized the miRNAs associated to each of the SMs. By validating our results utilizing an independent dataset we obtained an area under the ROC curve greater than 0.7. Furthermore, comparisons indicated our integrated approach significantly improved the identification performance of those simple modeled methods. This computational framework as well as the prioritized SM-miRNA targeting relationships will promote the further developments of targeted cancer therapies.Contact: yxli@sibs.ac.cn, lixia@hrbmu.edu.cn or jiangwei@hrbmu.edu.cnSupplementary information: Supplementary data are available at Bioinformatics online.
The application of a novel ‘rising activity, multi-level mixed effects, indicator emphasis’ (RAMMIE) method for syndromic surveillance in England
Authors: Morbey Roger A. , Elliot Alex J. , Charlett Andre , Verlander Neville Q. , Andrews Nick , Smith Gillian E. 
Published: July 2015

Abstract
Motivation: Syndromic surveillance is the real-time collection and interpretation of data to allow the early identification of public health threats and their impact, enabling public health action. The ‘rising activity, multi-level mixed effects, indicator emphasis’ method was developed to provide a single robust method enabling detection of unusual activity across a wide range of syndromes, nationally and locally.
Results: The method is shown here to have a high sensitivity (92%) and specificity (99%) compared to previous methods, whilst halving the time taken to detect increased activity to 1.3 days.
Availability and implementation: The method has been applied successfully to syndromic surveillance systems in England providing realistic models for baseline activity and utilizing prioritization rules to ensure a manageable number of ‘alarms’ each day.
Contact: roger.morbey@phe.gov.uk
Big biomedical data as the key resource for discovery science
Authors: Toga Arthur W , Foster Ian , Kesselman Carl , Madduri Ravi , Chard Kyle , Deutsch Eric W , Price Nathan D , Glusman Gustavo , Heavner Benjamin D , Dinov Ivo D , Ames Joseph , Van Horn John , Kramer Roger , Hood Leroy 
Published: July 2015

Abstract
Modern biomedical data collection is generating exponentially more data in a multitude of formats. This flood of complex data poses significant opportunities to discover and understand the critical interplay among such diverse domains as genomics, proteomics, metabolomics, and phenomics, including imaging, biometrics, and clinical data. The Big Data for Discovery Science Center is taking an “-ome to home” approach to discover linkages between these disparate data sources by mining existing databases of proteomic and genomic data, brain images, and clinical assessments. In support of this work, the authors developed new technological capabilities that make it easy for researchers to manage, aggregate, manipulate, integrate, and model large amounts of distributed data. Guided by biological domain expertise, the Center’s computational resources and software will reveal relationships and patterns, aiding researchers in identifying biomarkers for the most confounding conditions and diseases, such as Parkinson’s and Alzheimer’s.