bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma Levels of Aminothiols, Nitrite, Nitrate, and Malondialdehyde in Myelodysplastic Syndromes in the Context of Clinical Outcomes and as a Consequence of Iron Overload
Authors: Pimková Kristýna , Chrastinová Leona , Suttnar Jiří , Štikarová Jana , Kotlín Roman , Čermák Jaroslav , Dyr Jan Evangelista 
Published: January 2014

The role of oxidative stress in the initiation and progression of myelodysplastic syndromes (MDS) as a consequence of iron overload remains unclear. In this study we have simultaneously quantified plasma low-molecular-weight aminothiols, malondialdehyde, nitrite, and nitrate and have studied their correlation with serum iron/ferritin levels, patient treatment (chelation therapy), and clinical outcomes. We found significantly elevated plasma levels of total, oxidized, and reduced forms of cysteineP<0.001, homocysteineP<0.001, and cysteinylglycineP<0.006and significantly depressed levels of total and oxidized forms of glutathioneP<0.03and nitriteP<0.001in MDS patients compared to healthy donors. Moreover, total(P=0.032)and oxidized cysteinylglycine(P=0.029)and nitrite(P=0.021)differed significantly between the analyzed MDS subgroups with different clinical classifications. Malondialdehyde levels in plasma correlated moderately with both serum ferritin levels(r=0.78, P=0.001)and serum free iron levels(r=0.60, P=0.001)and were significantly higher in patients with iron overload. The other analyzed compounds lacked correlation with iron overload (represented by serum iron/ferritin levels). For the first time our results have revealed significant differences in the concentrations of plasma aminothiols in MDS patients, when compared to healthy donors. We found no correlation of these parameters with iron overload and suggest the role of oxidative stress in the development of MDS disease.
VEGF-Mediated Angiogenesis Links EMT-Induced Cancer Stemness to Tumor Initiation
Authors: Fantozzi Anna , Gruber Dorothea C. , Pisarsky Laura , Heck Chantal , Kunita Akiko , Yilmaz Mahmut , Meyer-Schaller Nathalie , Cornille Karen , Hopfer Ulrike , Bentires-Alj Mohamed , Christofori Gerhard 
Published: January 2014

Abstract
An epithelial–mesenchymal transition (EMT) underlies malignant tumor progression and metastatic spread by enabling cancer cells to depart from the primary tumor, invade surrounding tissue, and disseminate to distant organs. EMT also enriches for cancer stem cells (CSC) and increases the capacity of cancer cells to initiate and propagate tumors upon transplantation into immune-deficient mice, a major hallmark of CSCs. However, the molecular mechanisms promoting the tumorigenicity of cancer cells undergoing an EMT and of CSCs have remained widely elusive. We here report that EMT confers efficient tumorigenicity to murine breast cancer cells by the upregulated expression of the proangiogenic factor VEGF-A and by increased tumor angiogenesis. On the basis of these data, we propose a novel interpretation of the features of CSCs with EMT-induced, VEGF-A–mediated angiogenesis as the connecting mechanism between cancer cell stemness and tumor initiation. Cancer Res; 74(5); 1566–75. ©2014 AACR.
Future Opportunities in Cancer Nanotechnology—NCI Strategic Workshop Report
Authors: Grodzinski Piotr , Farrell Dorothy 
Published: January 2014

Abstract
There has been significant progress in utilizing nanotechnology in several areas of cancer care, including in vitro diagnostics, imaging, and therapy. The National Cancer Institute, which currently supports an array of research activities in cancer nanotechnology, convened a strategic workshop to explore the most promising directions and areas for future resource investment. The major discussion points as well as the opportunities identified are presented herein. Cancer Res; 74(5); 1307–10. ©2014 AACR.
Role of CHD5 in Human Cancers: 10 Years Later
Authors: Kolla Venkatadri , Zhuang Tiangang , Higashi Mayumi , Naraparaju Koumudi , Brodeur Garrett M. 
Published: January 2014

Abstract
CHD5 was first identified because of its location on 1p36 in a region of frequent deletion in neuroblastomas. CHD5 (chromodomain–helicase–DNA–binding-5) is the fifth member of a family of chromatin remodeling proteins, and it probably functions by forming a nucleosome remodeling and deacetylation (NuRD) complex that regulates transcription of particular genes. CHD5 is preferentially expressed in the nervous system and testis. On the basis of its position, pattern of expression, and function in neuroblastoma cells and xenografts, CHD5 was identified as a tumor suppressor gene (TSG). Evidence soon emerged that CHD5 also functioned as a TSG in gliomas and a variety of other tumor types, including breast, colon, lung, ovary, and prostate cancers. Although one copy of CHD5 is deleted frequently, inactivating mutations of the remaining allele are rare. However, DNA methylation of the CHD5 promoter is found frequently, and this epigenetic mechanism leads to biallelic inactivation. Furthermore, low CHD5 expression is strongly associated with unfavorable clinical and biologic features as well as outcome in neuroblastomas and many other tumor types. Thus, based on its likely involvement as a TSG in neuroblastomas, gliomas, and many common adult tumors, CHD5 may play an important developmental role in many other tissues besides the nervous system and testis. Cancer Res; 74(3); 652–8. ©2014 AACR.
KIF3a Promotes Proliferation and Invasion via Wnt Signaling in Advanced Prostate Cancer
Authors: Liu Zun , Rebowe Ryan E. , Wang Zemin , Li Yingchun , Wang Zehua , DePaolo John S. , Guo Jianhui , Qian Chiping , Liu Wanguo 
Published: January 2014

Abstract
Aberrant activation of the Wnt/β-catenin signaling pathway is a critical event in advanced prostate cancer, but the genetic alterations that activate the Wnt signaling pathway in many other cancers are rarely observed in prostate cancer. Other molecular mechanisms that regulate the Wnt signaling pathway in prostate cancer remain to be identified. Here, it is demonstrated that KIF3a, a subunit of kinesin-II motor protein, functions as an agonist of the Wnt signaling pathway in prostate cancer. KIF3a is upregulated in the majority of human prostate cancer cell lines and primary tumor biopsies. The expression levels of KIF3a correlate with a higher Gleason score, tumor–node–metastasis stage, and metastatic status of prostate cancer. Moreover, exogenous expression of KIF3a promoted cell growth in the benign prostate cells, whereas silencing KIF3a in cancer cells decreased cell proliferation, anchorage-independent cell growth, and cell migration/invasion. Mechanistically, KIF3a increases CK1-dependent DVL2 phosphorylation and β-catenin activation in prostate cancer cells, leading to transactivation of the Wnt-signaling target genes such as cyclin D1, HEF1, and MMP9. These findings support the notion that upregulation of KIF3a is causal of aberrant activation of Wnt signaling in advanced prostate cancer through the KIF3a–DVL2–β-catenin axis.
Implications: Inactivation of KIF3a may improve survival of patients with advanced prostate cancer in which Wnt signaling is activated. Mol Cancer Res; 12(4); 491–503. ©2014 AACR.
Prevalence, Significance, and Management of Aortic Insufficiency in Continuous Flow Left Ventricular Assist Device Recipients
Authors: Jorde Ulrich P. , Uriel Nir , Nahumi Nadav , Bejar David , Gonzalez-Costello Jose , Thomas Sunu S. , Han Jason , Morrison Kerry A. , Jones Sophie , Kodali Susheel , Hahn Rebecca T. , Shames Sofia , Yuzefpolskaya Melana , Colombo Paolo , Takayama Hiroo , Naka Yoshifumi 
Published: January 2014

Background—
Aortic insufficiency (AI) is increasingly recognized as a complication of continuous flow left ventricular assist device support; however, its long-term prevalence, clinical significance, and efficacy of potential interventions are not well known.

Methods and Results—
We studied the prevalence and management of AI in 232 patients with continuous flow left ventricular assist device at our institution. Patients with aortic valve (AV) surgery before left ventricular assist device implantation were excluded from analysis. To examine the prevalence of de novo AI, patients without preoperative AI were divided into a retrospective and a prospective cohort based on whether a dedicated speed optimization study had been performed at the time of discharge. Forty-three patients underwent AV repair at the time of implant, and 3 subsequently developed greater than mild AI. In patients without surgical AV manipulation and no AI at the time of implant, Kaplan–Meier analysis revealed that freedom from greater than mild de novo AI at 1 year was 77.6±4.2%, and that at least moderate AI is expected to develop in 37.6±13.3% after 3 years. Nonopening of the AV was strongly associated with de novo AI development in patients without prospective discharge speed optimization. Seven of 21 patients with at least moderate AI developed symptomatic heart failure requiring surgical intervention.

Conclusions—
AI is common in patients with continuous flow left ventricular assist devices and may lead to clinical decompensation requiring surgical correction. The prevalence of AI is substantially less in patients whose AV opens, and optimized loading conditions may reduce AI prevalence in those patients in whom AV opening cannot be achieved.
Trends in Monthly Tropopause Characteristics Observed over Taipei, Taiwan*
Authors: Raman M. Roja , Chen Wei-Nai 
Published: January 2014

Abstract
This study presents monthly trends in the cold-point tropopause (CPT), calculated using three decades of radiosonde data from 1981 to 2010 over Taipei, Taiwan (25°01′N, 121°27′E). Multivariate regression analysis has been used to suppress the effect of natural variations, such as quasi-biennial oscillation (QBO), ENSO, solar cycle, and volcanic eruptions. From the continuous time series, statistically insignificant heating and a decrease in the height of CPT are observed. However, the trends estimated using individual monthly time series revealed new features with statistically significant increasing trends in CPT temperature at a rate of approximately 0.03°C yr−1 and statistically significant decreasing trends in CPT height at a rate of approximately 4.7 m yr−1 during summer months. An enhanced heating rate in the upper troposphere along with a suppressed cooling rate in the lower stratosphere observed over Taipei might have caused the tropopause heating trend during summer. The possible relationship between tropopause trends and lower-stratospheric ozone is also examined. The seasonal and spatial variations in trends estimated using NCEP–Climate Forecast System Reanalysis (CFSR) data reveal the spatial heterogeneity in CPT temperature trends. Initial inspection of monthly trends in tropopause characteristics suggests that the estimation of tropopause trends using the continuous time series may not exactly represent the long-term variability of individual months or seasons.
How Does the East Asian Summer Monsoon Behave in the Decaying Phase of El Niño during Different PDO Phases?
Authors: Feng Juan , Wang Lin , Chen Wen 
Published: January 2014

Abstract
Modulation of the Pacific decadal oscillation (PDO) on the behavior of the East Asian summer monsoon (EASM) in El Niño decaying years has been studied. When El Niño is in phase with the PDO (El Niño/high PDO), the low-level atmospheric anomalies are characterized by an anticyclone around the Philippines and a cyclone around Japan, inducing an anomalous tripolar rainfall pattern in China. In this case, the western Pacific subtropical high (WPSH) experiences a one-time slightly northward shift in July and then stays stationary from July to August. The corresponding anomalous tripolar rainfall pattern has weak subseasonal variations. When El Niño is out of phase with the PDO (El Niño/low PDO), however, the anomalous Philippines anticyclone has a much larger spatial domain, thereby causing an anomalous dipole rainfall pattern. Accordingly, WPSH experiences clearly two northward shifts. Therefore, the related dipole rainfall pattern has large subseasonal variations. One pronounced feature is that the positive rainfall anomalies shift northward from southern China in June to central China in July and finally to northern China in August.
The different El Niño–EASM relationships are caused by the influences of PDO on the decaying speed of El Niño. During the high PDO phase, El Niño decays slowly and has a strong anchor in the north Indian Ocean warming, which is responsible for the anomalous EASM. Comparatively, during the low PDO phase, El Niño decays rapidly and La Niña develops in summer, which induces different EASM anomalies from that during the high PDO phase. Additionally, PDO changes El Niño behaviors mainly via modifying the background tropical winds.
The Regional Climate Impact of a Realistic Future Deforestation Scenario in the Congo Basin
Authors: Akkermans Tom , Thiery Wim , Van Lipzig Nicole P. M. 
Published: January 2014

Abstract
The demand for agricultural land in the Congo basin is expected to yield substantial deforestation over the coming decades. Although several studies exist on the climatological impact of deforestation in the Congo basin, deforestation scenarios that are implemented in climate models are generally crude. This study aims to refine current impact assessments by removing the primary forest according to an existing spatially explicit scenario, and replacing it by successional vegetation typically observed for the Congo basin. This is done within the Consortium for Small-Scale Modeling (COSMO) model in climate mode (COSMO-CLM), a regional climate model at 25-km grid spacing coupled to a state-of-the-art soil–vegetation–atmosphere transfer scheme (Community Land Model). An evaluation of the model shows good performance compared to in situ and satellite observations. Model integrations indicate that the deforestation, expected for the middle of the twenty-first century, induces a warming of about 0.7°C. This is about half the greenhouse gas–induced surface warming in this region, given an intermediate emission scenario (A1B) with COSMO-CLM driven by the ECHAM5 global climate model. This shows the necessity of taking into account deforestation to obtain realistic future climate projections. The deforestation-induced warming can be attributed to reduced evaporation, but this effect is mitigated by increased albedo and increased sensible heat loss to the atmosphere. Precipitation is also affected: as a consequence of surface warming resulting from deforestation, a regional heat low develops over the rain forest region. Resulting low-level convergence causes a redistribution of moisture in the boundary layer and a stabilization of the atmospheric column, thereby reducing convection intensity and hence precipitation by 5%–10% in the region of the heat low.
Stratocumulus Clouds in Southeastern Pacific Simulated by Eight CMIP5–CFMIP Global Climate Models
Authors: Lin Jia-Lin , Qian Taotao , Shinoda Toshiaki 
Published: January 2014

Abstract
This study examines the stratocumulus clouds and associated cloud feedback in the southeast Pacific (SEP) simulated by eight global climate models participating in phase 5 of the Coupled Model Intercomparison Project (CMIP5) and Cloud Feedback Model Intercomparison Project (CFMIP) using long-term observations of clouds, radiative fluxes, cloud radiative forcing (CRF), sea surface temperature (SST), and large-scale atmosphere environment. The results show that the state-of-the-art global climate models still have significant difficulty in simulating the SEP stratocumulus clouds and associated cloud feedback. Comparing with observations, the models tend to simulate significantly less cloud cover, higher cloud top, and a variety of unrealistic cloud albedo. The insufficient cloud cover leads to overly weak shortwave CRF and net CRF. Only two of the eight models capture the observed positive cloud feedback at subannual to decadal time scales. The cloud and radiation biases in the models are associated with 1) model biases in large-scale temperature structure including the lack of temperature inversion, insufficient lower troposphere stability (LTS), and insufficient reduction of LTS with local SST warming, and 2) improper model physics, especially insufficient increase of low cloud cover associated with larger LTS. The two models that arguably do best at simulating the stratocumulus clouds and associated cloud feedback are the only ones using cloud-top radiative cooling to drive boundary layer turbulence.
A Robust Estimation Method for Correcting Dynamic Draft Error in PPK GPS Elevation Using ADCP Tilt Data
Authors: Matte Pascal , Secretan Yves , Morin Jean 
Published: January 2014

Abstract
Measuring temporal and spatial variations in water level with high resolution and accuracy can provide fundamental insights into the hydrodynamics of marine and riverine systems. Real-time kinematic global positioning systems (RTK GPS), and by extension postprocessed kinematic (PPK) positioning, have provided the opportunity to achieve this goal, by allowing fast and straightforward measurements with subdecimeter accuracy. However, boat-mounted GPS are subject to movements of the water surface (e.g., waves, long-period heaves) as well as to the effects of dynamic draft. The latter contaminate the records and need to be separated and removed from the data. A method is proposed to postcorrect the elevation data using tilt information measured by an attitude sensor—in this case, an acoustic Doppler current profiler (ADCP) equipped with internal pitch and roll sensors. The technique uses iteratively reweighted least squares (IRLS) regressions to determine the position of the center of rotation (COR) of the boat that leads to optimal corrections. The COR is also allowed to change in time by performing the IRLS analyses on data subsamples, thus accounting for changes in weight distribution, for example, due to personnel movements. An example of application is presented using data collected in the Saint Lawrence fluvial estuary. The corrections exhibit significant reductions associated with the boat motion while keeping subtle variations in water levels likely related to local hydrodynamics.
A Case Study in Modeling Low-Lying Inversions and Stratocumulus Cloud Cover in the Bay of Biscay
Authors: Possner Anna , Zubler Elias , Fuhrer Oliver , Lohmann Ulrike , Schär Christoph 
Published: January 2014

Abstract
Many regional forecasting models struggle to simulate low-lying strong temperature inversions. To understand this apparent deficit for forecast improvements, a case study of a strong inversion occurring in the Bay of Biscay on 27 January 2003 is conducted. The event was characterized by extensive stratocumulus cloud cover beneath an extensive high pressure system in combination with a particularly strong inversion of 10–12 K at an altitude of 500–800 m. Simulations were performed at 2- and 12-km horizontal resolutions, with 60 vertical levels (13 levels within the first 1000 m), and with lead times of 12–72 h. The simulations were validated using in situ radiosonde and satellite data. Besides large-scale subsidence, turbulent vertical mixing is a key dynamical process for the formation of nocturnal inversions. Sensitivities to parameters for vertical mixing (the minimum threshold for eddy diffusivity and the turbulence length scale) are investigated. Results presented herein show the planetary boundary layer (PBL) profiles to be very sensitive to the minimum threshold applied for eddy diffusivity, whereas little sensitivity with respect to the turbulence length-scale parameter was found. PBL moisture and potential temperature θ profiles for hindcasts between 24- and 72-h lead times at both resolutions were adequately simulated. In simulations with an adequate representation of the vertical turbulent exchange, realistic cloud cover was simulated, while too high values of the aforementioned threshold produced a strong underestimation of the cloud cover. These results indicate that a realistic simulation of strong inversions and their associated cloud cover is feasible, provided the vertical turbulent exchange is adequately represented.
Accuracy of general practitioner unassisted detection of depression
Authors: Carey Mariko , Jones Kim , Meadows Graham , Sanson-Fisher Rob , D’Este Catherine , Inder Kerry , Yoong Sze Lin , Russell Grant 
Published: January 2014

Objective: Primary care is an important setting for the treatment of depression. The aim of the study was to describe the accuracy of unassisted general practitioner judgements of patients’ depression compared to a standardised depression-screening tool delivered via touch-screen computer. Method: English-speaking patients, aged 18 or older, completed the Patient Health Questionnaire-9 (PHQ-9) when presenting for care to one of 51 general practitioners in Australia. General practitioners were asked whether they thought the patients were clinically depressed. General practitioner judgements of depression status were compared to PHQ-9 results. Results: A total of 1558 patients participated. Twenty per cent of patients were identified by the PHQ-9 as being depressed. General practitioners estimated a similar prevalence; however, when compared to the PHQ-9, GP judgement had a sensitivity of 51% (95% CI [32%, 66%]) and a specificity of 87% (95% CI [78%, 93%]). Conclusions: General practitioner unassisted judgements of depression in their patients lacked sensitivity when compared to a standardised psychiatric measure used in general practice.
Paediatric day case tonsillectomy: a safe, feasible and an economical way to treat patients – Yorkhill experience
Authors: Elsobky S , Ahmad N , Qureshi M , Izzath W , Sadiq H 
Published: January 2014

Introduction Day case surgery is widely encouraged as it has many benefits. In this study, we evaluate the feasibility of paediatric tonsillectomies as day case surgery procedures in a tertiary paediatric centre. Methods The outcome of paediatric tonsillectomy performed as day case procedures was studied for four months. We recorded complication rates in comparison to the standard in-patient practice. Results 23 paediatric patients were included in this study. The age of the patients ranged from four to 11 years, with mean age of 6.5. No patients suffered post-operative complications within the first 24 h. Two patients were re-admitted due to a post-operative complication (8.6%) and one patient for a non-operative cause (4.3%) beyond the initial 24 h. Conclusion Paediatric Day Case Paediatric Tonsillectomy is a safe, feasible and an economical way to manage patients. It should be practised as a day case where appropriate and when criteria are met.
Florence Nightingale and Mary Seacole: Which is the forgotten hero of health care and why?
Authors: McDonald L 
Published: January 2014

Background and aims This paper aims at correcting misinformation in circulation portraying Mary Seacole as a nurse, Crimean War heroine, and health care pioneer, even, for some, a replacement for Florence Nightingale, who really was a health care pioneer as well as being the major founder of the modern profession of nursing. Methods and results The article focuses on the claims for Seacole made by C. Short in Scottish Medical Journal, 2011. It reports, using primary sources, on what Seacole actually did – running a business for officers, with kind acts on the side – short of constituting heroism, pioneering health care or nursing. Conclusion The article concludes with remarks on how Nightingale came to be forgotten as a health care pioneer, with comments on the two major sources that attacked her reputation, F.B. Smith in 1982, and Hugh Small in 1998. Detailed refutations in peer-reviewed sources are referenced. Finally, it is suggested that recent scandals in English hospital care, documented in the Francis Report, may provoke a revival of interest in Nightingale’s principles and methods.