bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prospective longitudinal controlled assessment of pain experience and oral health‐related quality of life in adolescents undergoing fixed appliance treatment
Authors: Johal A. , Fleming P. S. , Al Jawad F. A. 
Published: April 2014

Structured AbstractObjectiveTo compare subjective pain experience and oral health‐related quality of life (OH‐QoL) in treated and untreated subjects over the first 3 months of fixed appliance therapy.Setting and Sample PopulationThe Department of Orthodontics, School of Medicine and Dentistry. One hundred and twenty‐four subjects aged between 11 and 14 years either commencing or awaiting fixed appliance treatment.Material & MethodsA prospective controlled longitudinal study design was applied to subjects, over a 3‐month observation period, following the placement of fixed appliances. Socio‐economic status, OH‐QoL, pain experience and analgesic consumption were recorded on questionnaires at baseline (T0), 6 weeks (T1) and 3 months (T2).ResultsOral symptoms and functional limitation domains of OH‐QoL were found to worsen, during the follow‐up period, in the test group (p = 0.001 and p = 0.002, respectively). In the treated group, pain intensity declined significantly on days 3 and 2 at T1 and T2, respectively (p < 0.001). Analgesia was required during both periods in a total of 13 participants (24.5%) undergoing orthodontic treatment.ConclusionBased on this prospective controlled study, the initial stages of fixed appliance treatment results in subjective pain experience, with subsequent reduction, and a significant impact on oral symptoms and functional limitation domains of OH‐QoL.
Near‐genomewide RNAi screening for regulators of BRAFV600E‐induced senescence identifies RASEF, a gene epigenetically silenced in melanoma
Authors: Kaplon Joanna , Hömig‐Hölzel Cornelia , Gao Linda , Meissl Katrin , Verdegaal Els M. E. , van der Burg Sjoerd H. , van Doorn Remco , Peeper Daniel S. 
Published: April 2014

SummaryThe activation of oncogenes in primary cells blocks proliferation by inducing oncogene‐induced senescence (OIS), a highly potent in vivo tumor‐suppressing program. A prime example is mutant BRAF, which drives OIS in melanocytic nevi. Progression to melanoma occurs only in the context of additional alteration(s) like the suppression of PTEN, which abrogates OIS. Here, we performed a near‐genomewide short hairpin (sh)RNA screen for novel OIS regulators and identified by next generation sequencing and functional validation seven genes. While all but one were upregulated in OIS, depletion of each of them abrogated BRAFV600E‐induced arrest. With genome‐wide DNA methylation analysis, we found one of these genes, RASEF, to be hypermethylated in primary cutaneous melanomas but not nevi. Bypass of OIS by depletion of RASEF was associated with suppression of several senescence biomarkers including senescence‐associated (SA)‐β‐galactosidase activity, interleukins, and tumor suppressor p15INK4B. Restoration of RASEF expression inhibited proliferation. These results illustrate the power of shRNA OIS bypass screens and identify a potential novel melanoma suppressor gene.
Language as the House of Being? How to Bring Intelligibility to Heidegger While Keeping the Excitement
Authors: Vandevelde Pol 
Published: April 2014

AbstractAt the core of Heidegger's philosophy, there lies this nagging question: what is the link between language and being? Using a famous formulation by Heidegger as a guide (‘When we go to the well, when we go through the woods, we are always already going through the word “well”, through the word “woods”’), the analysis focuses on the connection Heidegger establishes between being (what woods and well ‘are’), understanding (something is understood ‘as’ woods or well), and temporality (human understanding of woods and well has changed since ancient Greek times, for example). Language is both what grants things their way to matter to us and thus to ‘be’ for us to the extent that we understand them, but language is also linked to a ‘happening’ (Geschehen) or an event so that things are not bound to their Geek being or current being: things ‘become being’ (seiend werden). Language is both the means for things to materialize in historical times: ‘being woods’ or ‘being a well,’ as well as a testimony to their entry into being. This is why language in its poetic use can make beings ‘more being’ (seiender).
Immune response to Cystoisospora suis in piglets: local and systemic changes in T‐cell subsets and selected mRNA transcripts in the small intestine
Authors: Gabner S. , Worliczek H. L. , Witter K. , Meyer F. R. L. , Gerner W. , Joachim A. 
Published: April 2014

SummaryInfections of neonatal piglets with Cystoisospora suis are responsible for substantial economic losses in pig production. To investigate kinetics of T‐cell populations, which are possibly involved in this infection, lymphocytes from blood, spleen, mesenteric lymph nodes and the jejunal mucosa of infected and noninfected piglets were investigated by flow cytometry and immunohistochemistry at five time points during the acute phase of primary infection. Additionally, mRNA expression levels of pattern recognition receptors and immunomodulatory cytokines in the jejunum were investigated. T‐cell receptor‐γδ+ T cells were found to be increased in the gut mucosa 4 days after infection and were most likely involved in the primary local immune response. Five to eleven days later, cytotoxic T cells peaked in this location, which was preceded by an expansion of this lymphocyte population in the mesenteric lymph nodes. In intestines of infected piglets, mRNA expressions of TLR‐2, NOD2 and TNF‐α were significantly upregulated, suggesting an involvement in parasite recognition, immune response and possibly also in immunopathology. Taken together, this study identifies cellular and molecular players involved in the early immune responses against C. suis, but their precise role in the pathogenesis and control of this neonatal disease requires further investigation.
Maternal Stressors and Social Support as Risks for Delivering Babies with Structural Birth Defects
Authors: Carmichael Suzan L. , Ma Chen , Tinker Sarah , Rasmussen Sonia A. , Shaw Gary M. ,   National Birth Defects Prevention Study
Published: April 2014

AbstractBackgroundWe examined the association of maternal stressful life events and social support with risks of birth defects using National Birth Defects Prevention Study data, a population‐based case–control study.MethodsWe examined seven stressful life events and three social support questions applicable to the periconceptional period, among mothers of 552 cases with neural tube defects (NTDs), 413 cleft palate (CP), 797 cleft lip ± cleft palate (CLP), 189 d‐transposition of the great arteries (dTGA), 311 tetralogy of Fallot (TOF), and 2974 non‐malformed controls. A stressful life events index equalled the sum of ‘yes’ responses to the seven questions. Social support questions were also summed to form an index. Data were analyzed using logistic regression to estimate odds ratios (OR) and 95% confidence intervals (CI), adjusted for maternal race‐ethnicity, age, education, body mass index, smoking, drinking, and intake of vitamin supplements.ResultsAssociations with the stress index tended to be higher with higher scores, but few 95% CIs excluded one. A four‐point increase in the index was moderately associated with NTDs (OR 1.5, [95% CI 1.1, 2.0]) and CLP (OR 1.3, [95% CI 1.0, 1.7]). The social support index tended to be associated with reduced risk but most 95% CIs included one, with the exception of dTGA (OR for a score of 3 vs 0 was 0.5 [95% CI 0.3, 0.8]).ConclusionsMaternal periconceptional stressful life events, social support, and the two factors in combination were at most modestly, if at all, associated with risks of the studied birth defects.
Dietary Fat Supplementation and the Consequences for Oocyte and Embryo Quality: Hype or Significant Benefit for Dairy Cow Reproduction?
Authors: Leroy JLMR , Sturmey RG , Van Hoeck V , De Bie J , McKeegan PJ , Bols PEJ 
Published: April 2014

ContentsIn many countries, fat supplementation in the diet has become common in the dairy industry. There are several ideas as to how dietary fat could influence reproductive performance. Saturated fatty acids, such as palm oil, can increase milk yield but may aggravate negative energy balance and thus may impair fertility when fed during the first week post‐partum. However, priming the lipid oxidation in the liver by feeding saturated fats during the dry period has recently been shown to be a potentially promising strategy to mitigate fat mobilization and liver accumulation post‐partum. Furthermore, polyunsaturated fats (omega‐3 fatty acids and conjugated linoleic acids) are fed to reduce the ‘de novo’ fat synthesis in the udder and thus the milk fat content, which may be of modest benefit for overall energy balance. Furthermore, omega‐6 and omega‐3 polyunsaturated fatty acids are reported to alter follicular growth, steroid synthesis and prostaglandin metabolism in the ovary and endometrium, respectively. Omega‐6 fatty acids are believed to have pro‐inflammatory and thus PGF2α‐stimulating properties rendering them extra value as ‘nutraceutical’ early post‐partum, while omega‐3 fatty acids can weaken this inflammatory potency, leading to a higher chance of survival of the embryo when supplemented during the periconceptual period. Unfortunately, research results rarely provide a consensus in this perspective. The consequences of these fat‐feeding strategies on oocyte and embryo quality remain an intriguing issue for debate. Fat feeding may alter the microenvironment of the growing and maturing oocyte of the early and older embryo and thus may affect reproductive outcome. We recently reported that dietary‐induced hyperlipidaemic conditions can be harmful for embryo development and metabolism. However, to date, research results remain somewhat conflicting most probably due to differences in fat sources used, in diet and duration of supplementation and in experimental set‐up in general.
Oral health status of individuals with cerebral palsy at a nationally recognized rehabilitation center
Authors: Al‐Allaq Tumouh , DeBord Terrie K. , Liu Honghu , Wang Yan , Messadi Diana V. 
Published: April 2014

ABSTRACTCerebral palsy (CP) is a set of nonprogressive neuromuscular disorders caused by defects in the developing fetal brain. The aim of this study is to investigate the prevalence and distribution of various dental conditions including dental caries and periodontitis among individuals with CP who receive care at the Rancho Los Amigos National Rehabilitation Center dental clinic.Medical records of 478 patients between the ages of 3 and 78 years were reviewed. Patients were divided into four age groups: 3–20, 21–35, 36–55, and 56 and above year old. Data related to their dental conditions including caries, periodontitis, and other oral diseases were assessed. Statistical analyses were conducted to evaluate the correlations between these oral diseases and age, gender, ethnicity as well as their living conditions (home or group home).The 36–55‐year‐old age group displayed significantly more caries and periodontitis than any other age groups. Individuals aged 3–20 years showed a significantly lower rate of periodontitis and caries. There was no significant association between gender and race with these outcome variables but there was a correlation between these variables and living conditions.Differences in oral health exist among people with CP from different age groups and living conditions. These findings suggest that there is a dire need for more oral hygiene training and education for the care givers. Dental schools should better prepare their graduates to meet the treatment demands of individuals with special healthcare needs.
Reproductive risk factors assessment for anaemia among pregnant women in India using a multinomial logistic regression model
Authors: Perumal Vanamail 
Published: April 2014

AbstractObjectiveTo assess reproductive risk factors for anaemia among pregnant women in urban and rural areas of India.MethodThe International Institute of Population Sciences, India, carried out third National Family Health Survey in 2005–2006 to estimate a key indicator from a sample of ever‐married women in the reproductive age group 15–49 years. Data on various dimensions were collected using a structured questionnaire, and anaemia was measured using a portable HemoCue instrument. Anaemia prevalence among pregnant women was compared between rural and urban areas using chi‐square test and odds ratio. Multinomial logistic regression analysis was used to determine risk factors.ResultsAnaemia prevalence was assessed among 3355 pregnant women from rural areas and 1962 pregnant women from urban areas. Moderate‐to‐severe anaemia in rural areas (32.4%) is significantly more common than in urban areas (27.3%) with an excess risk of 30%. Gestational age specific prevalence of anaemia significantly increases in rural areas after 6 months. Pregnancy duration is a significant risk factor in both urban and rural areas. In rural areas, increasing age at marriage and mass media exposure are significant protective factors of anaemia. However, more births in the last five years, alcohol consumption and smoking habits are significant risk factors.ConclusionIn rural areas, various reproductive factors and lifestyle characteristics constitute significant risk factors for moderate‐to‐severe anaemia. Therefore, intensive health education on reproductive practices and the impact of lifestyle characteristics are warranted to reduce anaemia prevalence.
Impact of ethnicity on human umbilical cord blood banking: a systematic review
Authors: Akyurekli Celine , Chan Joshua Y.S. , Elmoazzen Heidi , Tay Jason , Allan David S. 
Published: April 2014

BackgroundHow ethnicity impacts characteristics of umbilical cord blood collected by cord blood banks remains unclear.Study Design and MethodsAfter a systematic search, we identified 11 studies for analysis that reported on various variables of collected cord blood units.ResultsNon‐Caucasian ethnicity of the cord blood donor was associated with a higher risk of failing to meet banking criteria, lower cord blood volume, reduced total nucleated cell count, fewer CD34+ cells, and reduced colony‐forming units.ConclusionsNon‐Caucasian ethnicity is associated with reductions in hematopoietic measures of collected cord blood units. Public banking efforts will have to balance issues related to building ethnic diversity within the bank and maintaining the characteristics of banked units that remain desirable by transplant centers.
Draft Genome Sequences of Two
Arcobacter
Strains Isolated from Human Feces
Authors: Adam Zaky , Whiteduck-Léveillée Kerri , Cloutier Michel , Chen Wen , Lewis Christopher T. , Lévesque C. André , Topp Edward , Lapen David R. , Tambong James T. , Talbot Guylaine , Khan Izhar U. H. 
Published: April 2014

ABSTRACT

Arcobacter
species are members of the family
Campylobacteraceae
and are considered emerging enteropathogens and potential zoonotic agents. Here, we report the draft genome sequences of two
Arcobacter
strains isolated from human feces in an effort to provide further genetic resources for understanding the pathogenic dynamics and diversity of this important genus.
Genome Sequence of the Acrystalliferous Bacillus thuringiensis Serovar Israelensis Strain 4Q7, Widely Used as a Recombination Host
Authors: Jeong Haeyoung , Park Seung-Hwan , Choi Soo-Keun 
Published: April 2014

ABSTRACT

Bacillus thuringiensis
serovar israelensis is well known for its mosquitocidal activity and has long been used as a biopesticide. Herein, we present the genome sequence of
B. thuringiensis
serovar israelensis strain 4Q7, a plasmid-cured derivative with higher transformation efficiency than wild types.
Improved Hybrid Genome Assemblies of Two Strains of Bacteroides xylanisolvens, SD_CC_1b and SD_CC_2a, Obtained Using Illumina and 454 Sequencing Technologies
Authors: Ramaraj Thiruvarangan , Sundararajan Anitha , Schilkey Faye D. , DelVecchio Vito G. , Donlon Mildred , Ziemer Cherie , Mudge Joann 
Published: April 2014

ABSTRACT

Bacteroides xlyanisolvens
strains (SD_CC_1b, SD_CC_2a) isolated from human feces were grown on crystalline cellulose. Cellulolytic properties are not common in
Bacteroides
species. Here, we report improved genome sequences of both of the
B. xlyanisolvens
strains.
De Novo
Assembly of the Quorum-Sensing
Pandoraea
sp. Strain RB-44 Complete Genome Sequence Using PacBio Single-Molecule Real-Time Sequencing Technology
Authors: Ee Robson , Lim Yan-Lue , Yin Wai-Fong , Chan Kok-Gan 
Published: April 2014

ABSTRACT

We report the first complete genome sequence of
Pandoraea
sp. strain RB-44, which was found to possess quorum-sensing properties. To the best of our knowledge, this is the first documentation of both a complete genome sequence and quorum-sensing properties of a
Pandoraea
species.
Complete Genome Sequence of a Porcine Circovirus 2 Strain Isolated in Central China
Authors: Guo Guan-Peng , Pan Xin-Long , Cheng Kun , Fu Peng-Fei , Yang Ming-Fan , Chen Hong-Ying 
Published: April 2014

ABSTRACT
We report here the complete genome sequence of the porcine circovirus type 2 (PCV2) XZBK strain, isolated from central China. This is the first report of a member of the 1B subgroup of PCV2b central China. This finding will help in understanding the epidemiology and molecular characteristics of PCV2.
Draft Genome Sequences of
Psychrobacter
Strains JCM 18900, JCM 18901, JCM 18902, and JCM 18903, Isolated Preferentially from Frozen Aquatic Organisms
Authors: Kudo Toshiaki , Kidera Akihiro , Kida Muneaki , Kawauchi Ayumi , Shimizu Ryo , Nakahara Tomomi , Zhang Xiaochi , Yamada Akinori , Amano Masao , Hamada Yuki , Taniyama Shigeto , Arakawa Osamu , Yoshida Asami , Oshima Kenshiro , Suda Wataru , Kuwahara Hirokazu , Nogi Yuichi , Kitamura Keiko , Yuki Masahiro , Iida Toshiya , Moriya Shigeharu , Inoue Tetsushi , Hongoh Yuichi , Hattori Masahira , Ohkuma Moriya 
Published: April 2014

ABSTRACT

Four
Psychrobacter
strains, JCM 18900, JCM 18901, JCM 18902, and JCM 18903, related to either
Psychrobacter nivimaris
or
Psychrobacter cibarius
, were isolated from frozen marine animals. The genome information of these four strains will be useful for studies of their physiology and adaptation properties to frozen conditions.
Correlation of the nappe stack in the Ibero-Armorican arc across the Bay of Biscay: a joint French–Spanish project
Authors: Ballèvre Michel , Martínez Catalán José R. , López-Carmona Alicia , Pitra Pavel , Abati Jacobo , Fernández Rubén Díez , Ducassou Céline , Arenas Ricardo , Bosse Valérie , Castiñeiras Pedro , Fernández-Suárez Javier , Gómez Barreiro Juan , Paquette Jean-Louis , Peucat Jean-Jacques , Poujol Marc , Ruffet Gilles , Sánchez Martínez Sonia 
Published: April 2014

Abstract
A correlation between allochthonous units exposed in the NW Iberian Massif and the southern Armorican Massif is carried out based on lithological associations, structural position, age and geochemistry of protoliths and tectonometamorphic evolution. The units on both sides of the Bay of Biscay are grouped into Upper, Middle and Lower allochthons, whereas an underlying allochthonous thrust sheet identified in both massifs is referred to as the Parautochthon. The Lower Allochthon represents a fragment of the outermost edge of Gondwana that underwent continental subduction shortly after the closure of a Palaeozoic ocean which, in turn, is represented by the Middle Allochthon. The latter consists of supra-subduction ophiolites and metasedimentary sequences alternating with basic, mid-ocean ridge basalt (MORB)-type volcanics, with inheritances suggesting the proximity of a continental domain. Seafloor spreading began at the Cambro-Ordovician boundary and oceanic crust was still formed during the Late Devonian, covering the lifetime of the Rheic Ocean, which is possibly represented by the Middle Allochthon. The opening of the oceanic domain was related to pulling apart the peri-Gondwanan continental magmatic arc, which is represented by the Upper Allochthon.
Motivational Processes in Online Learning: The Role of Tutorship for Laboratory Activities through the Semistructured Self-Evaluation Tests
Authors: Biasi Valeria , Domenici Gaetano 
Published: April 2014

This contribution highlights the role of  affectiveprocesses in multimedia learning conducted by e-learning platform used in the General Psychology online laboratory activities, based on semistructured self-evaluation tests following the model proposed by Domenici (2005). These tests include items with a closed- and open-ended structure requiring the student to answer within space restrictions and, in some cases, within context simulations. This enables scorers to give generally univocal and objective scores thereby assuring high test validity and reliability. The focus is on the psychological aspects of the online tutor’s role and its effects on learning levels. Students who completed the online laboratory activities through the semistructured self-evaluation tests (n=252) obtained an average score of 27.7 in the objective examination; students who did not complete the activities or who never even started them (n=186) obtained an average score of 25.7. These differences are statistically significant (F1, 449=38.05;P<0.001). The advantages of online learning are highlighted: developing an active attitude towards learning, enabling the individualisation of learning, and strengthening motivation with regard to knowledge, self-assertiveness, and sociality. This motivational activation reinforces convergent and divergent cognitive skills, owing to the semistructured tests, which facilitate knowledge and study method acquisition.
Cross-Cultural Validity, Reliability, and Psychometric Properties of the Persian Version of the Scales for Outcomes in Parkinson’s Disease-Psychosocial Questionnaire
Authors: Fereshtehnejad Seyed-Mohammad , Farhadi Farzaneh , Hadizadeh Hasti , Shahidi Gholam Ali , Delbari Ahmad , Lökk Johan 
Published: April 2014

Objectives. Considering the influence of different motor and nonmotor features of Parkinson’s disease (PD), it is important to evaluate the psychosocial functioning of the patients. For this purpose, the scales for outcomes in Parkinson’s disease-psychosocial questionnaire (SCOPA-PS) has been previously designed. The aim of our study was to assess the cross-cultural validation and psychometric properties of the Persian version of the SCOPA-PS.Methods. One hundred and ten nondemented idiopathic Parkinson’s disease (IPD) patients were consecutively recruited from an outpatient referral movement disorder clinic. Eligible patients filled up a number of questionnaires including the Persian version of SCOPA-PS during the face-to-face interview session and clinical examination to measure disease severity, nonmotor psychiatric symptoms, and health-related quality of life (HRQoL).Results. The highest and lowest correlation coefficients of internal consistency were reported for item 7 on “asking for help”(r=0.765)and item 5 on “sexual problems”(r=0.553). Cronbach’s alpha reliability coefficient of the entire scale was 0.87 (95% CI: 0.83–0.90). The Hoehn and Yahr stage(r=0.34, P<0.001), Schwab and England ADL scale(r=-0.55, P<0.001), anxiety(r=0.64, P<0.001), depression(r=0.71, P<0.001), and fatigue(r=0.35, P<0.001)were significantly correlated with the total score of the SCOPA-PS questionnaire.Conclusions. The Persian version of SCOPA-PS is a highly reliable and valid scale to measure psychosocial functioning in IPD patients with different sex, age-group, and educational level, which could be applied in future researches. Disease severity scales, depression, anxiety, fatigue, and different domains of HRQoL were all associated with psychosocial functioning in PD patients.