bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biomimetic hydroxyapatite coating on pore walls improves osteointegration of poly(L‐lactic acid) scaffolds
Authors: Deplaine H. , Lebourg M. , Ripalda P. , Vidaurre A. , Sanz‐Ramos P. , Mora G. , Prósper F. , Ochoa I. , Doblaré M. , Gómez Ribelles J. L. , Izal‐Azcárate I. , Gallego Ferrer G. 
Published: November 2012

AbstractPolymer–ceramic composites obtained as the result of a mineralization process hold great promise for the future of tissue engineering. Simulated body fluids (SBFs) are widely used for the mineralization of polymer scaffolds. In this work an exhaustive study with the aim of optimizing the mineralization process on a poly(L‐lactic acid) (PLLA) macroporous scaffold has been performed. We observed that when an air plasma treatment is applied to the PLLA scaffold its hydroxyapatite nucleation ability is considerably improved. However, plasma treatment only allows apatite deposition on the surface of the scaffold but not in its interior. When a 5 wt % of synthetic hydroxyapatite (HAp) nanoparticles is mixed with PLLA a more abundant biomimetic hydroxyapatite layer grows inside the scaffold in SBF. The morphology, amount, and composition of the generated biomimetic hydroxyapatite layer on the pores' surface have been analyzed. Large mineralization times are harmful to pure PLLA as it rapidly degrades and its elastic compression modulus significantly decreases. Degradation is retarded in the composite scaffolds because of the faster and extensive biomimetic apatite deposition and the role of HAp to control the pH. Mineralized scaffolds, covered by an apatite layer in SBF, were implanted in osteochondral lesions performed in the medial femoral condyle of healthy sheep. We observed that the presence of biomimetic hydroxyapatite on the pore's surface of the composite scaffold produces a better integration in the subchondral bone, in comparison to bare PLLA scaffolds. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 173–186, 2013.
Mixed algae cultures for low cost environmental compensation in cultures grown for lipid production and wastewater remediation
Authors: Johnson Kyle R , Admassu Wudneh 
Published: November 2012

AbstractBackgroundMost cultures of algae today are grown as monocultures. The primary reason for this appears to be that specific strains of algae contain the high‐value products that are desired for harvest. But, in algae cultures grown primarily for lipid production, or as a stage in wastewater remediation, mixed algae cultures may mitigate environmental risk.ResultsFour‐strain mixed cultures of algae were grown in simulated wastewater, alongside the monocultures, under different temperature and lighting conditions. Under all conditions the mixed cultures were less productive than the most fit monocultures for each situation, but more productive than the least fit monocultures. Statistical analysis based on an exponential growth model of the monocultures also suggested that mixed cultures are actually more productive both in terms of biomass and lipids than simply the sum of the monocultures.ConclusuionsA mixed culture composed of various similarly productive strains with various optimal growing conditions would be less susceptible to environmental shifts than a monoculture. This study demonstrated the promise of an appropriately defined mixed culture to reduce risk in applications such as wastewater remediation and lipid production that do not rely on specific strains containing high value products. © 2012 Society of Chemical Industry
Invasive biomass valorization: environmentally friendly processes for obtaining second generation bioethanol and saccharides from Ulex europæus
Authors: Ares‐Peón Iria Ana , Romaní Aloia , Garrote Gil , Parajó Juan Carlos 
Published: November 2012

AbstractBACKGROUNDUlex europæus (UE) is a widespread
invasive shrub species causing economic problems and environmental
hazards. This work deals with the valorization of UE by
hydrothermal processing (to obtain hemicelluloses‐derived
saccharides) followed by simultaneous saccharification and fermentation of the resulting solids for manufacturing second generation bioethanol.RESULTSHydrothermal processing of UE resulted in the
solubilization of up to 21.5 wt% of the oven‐dry raw
material, leading to the formation of hemicelluloses‐derived
saccharides as major reaction products. Treatments at various
severities resulted in processed solids with enhanced susceptibility to enzymatic hydrolysis, allowing cellulose to glucose conversions up to 87%. Simultaneous saccharification and fermentation of solids pretreated under selected conditions, performed at various charges and solid loadings, resulted in bioethanol conversions up to 82% of the stoichiometric amount, with volumetric concentrations higher than 30 g ethanol L‐1.CONCLUSIONHydrothermal processing of UE followed by simultaneous
saccharification and fermentation of pretreated solids was suitable
for the selective separation of hemicelluloses as soluble saccharides
and for the manufacture of second generation bioethanol at high yield
from the pretreated solids.© 2012 Society of Chemical Industry
Endometrial cancer: Utility of diffusion‐weighted magnetic resonance imaging with background body signal suppression at 3T
Authors: Seo Jung Min , Kim Chan Kyo , Choi Dongil , Kwan Park Byung 
Published: November 2012

AbstractPurpose:To prospectively assess the usefulness of diffusion‐weighted magnetic resonance imaging (MRI) with background body signal suppression (DWIBS) at 3T for the preoperative evaluation of endometrial cancer.Materials and Methods:Fifty‐two consecutive patients with biopsy‐proven endometrial cancer were examined with a 3T MR scanner, followed by a hysterectomy. MR examinations included T2‐weighted (T2WI), DWIBS, and dynamic contrast‐enhanced T1‐weighted imaging (DCEI). The apparent diffusion coefficient (ADC) was calculated in the tumor and normal myometrium. According to tumor grade, the mean ADC of the tumor was analyzed. The depth of myometrial invasion was independently assessed by two radiologists for three MRI datasets on a five‐point scale.Results:The mean ADC of the tumors was significantly lower than that of normal myometrium (P < 0.001). The mean ADC of grades 2 or 3 was significantly lower than grade 1 (P < 0.01). For predicting myometrial invasion, the specificity, accuracy, and area under the curve of combined T2WI and DWIBS in both readers were similar to DCEI (P > 0.05). Interreader agreement in all MRI datasets was excellent.Conclusion:DWIBS at 3T has potential for being an effective method for the preoperative evaluation of endometrial cancer. J. Magn. Reson. Imaging 2013;37:1151–1159. © 2012 Wiley Periodicals, Inc.
Subject tolerance of 7 T MRI examinations
Authors: Versluis Maarten J. , Teeuwisse Wouter M. , Kan Hermien E. , van Buchem Mark A. , Webb Andrew G. , van Osch Matthias J. 
Published: November 2012

Purpose:To determine the subjective experiences and the sources of discomfort for subjects undergoing 7 T magnetic resonance imaging (MRI) examinations on a whole‐body 7 T system in a hospital settingMaterials and Methods:A postscan survey was filled out by 101 healthy subjects who participated in a 7 T examination. All participants answered questions regarding different potential sensations of discomfort including dizziness, claustrophobia, and scanner noise.Results:Dizziness was reported most frequently, with 34% of subjects experiencing dizziness while moving into the scanner and 30% while moving out of the magnet. Scanner noise was also frequently mentioned as uncomfortable (33% of the subjects). In 11% of the cases a metallic taste was reported. The overall experience was rated by 3% as unpleasant, 51% as neutral, and 46% as pleasant.Conclusion:The reported side effects are larger than previously reported for lower field strengths. However, overall, 7 T examinations are well tolerated, with only 3% of subjects rating it as unpleasant. These results agree well with previous in‐depth studies, and provide further evidence that 7 T MRI would be accepted by patients in clinical practice. J. Magn. Reson. Imaging 2013;38:722–725. © 2012 Wiley Periodicals, Inc.
Corticomedullary differentiation of the kidney: Evaluation with noncontrast‐enhanced steady‐state free precession (SSFP) MRI with time‐spatial labeling inversion pulse (time‐SLIP)
Authors: Kanki Akihiko , Ito Katsuyoshi , Tamada Tsutomu , Noda Yasufumi , Yamamoto Akira , Tanimoto Daigo , Sato Tomohiro , Higaki Atsushi 
Published: November 2012

AbstractPurpose:To assess whether noncontrast‐enhanced steady‐state free precession (SSFP) magnetic resonance imaging (MRI) with time‐spatial labeling inversion pulse (Time‐SLIP) can improve the visibility of corticomedullary differentiation of the normal kidney.Materials and Methods:A series of noncontrast‐enhanced SSFP MRI with Time‐SLIP were performed in 20 patients by using various inversion times (TIs); 500–1800 msec in increments of 100 msec. In‐phase (IP) and opposed‐phase (OP) MR images were also obtained. The signal intensity (SI) of the renal cortex and medulla was measured to calculate corticomedullary contrast ratio (SI of cortex/medulla). Additionally, the visibility of corticomedullary differentiation was visually categorized using a four‐point scale.Results:In SSFP with Time‐SLIP, corticomedullary contrast ratio was highest with TI of 1200 msec in eight subjects (40%), followed by 1100 msec in seven (35%) and 1000 msec in three (15%). The corticomedullary contrast ratio in SSFP with optimal Time‐SLIP (4.93 ± 1.25) was significantly higher (P < 0.001) than those of IP (1.46 ± 0.12) and OP (1.43 ± 0.14). The visibility of corticomedullary differentiation was significantly better (P < 0.001) in SSFP images with Time‐SLIP (averaged grade = 4.0) than in IP images (averaged grade = 2.63) and OP images (averaged grade = 2.05).Conclusion:SSFP MRI with Time‐SLIP can improve the visibility of renal corticomedullary differentiation without using contrast agents. J. Magn. Reson. Imaging 2012;37:1178–1181. © 2012 Wiley Periodicals, Inc.
Impaired hypoxic sensor Siah‐1, PHD3, and FIH system in spinal motor neurons of an amyotrophic lateral sclerosis mouse model
Authors: Sato Kota , Morimoto Nobutoshi , Kurata Tomoko , Mimoto Takafumi , Miyazaki Kazunori , Ikeda Yoshio , Abe Koji 
Published: November 2012

AbstractWe recently reported spinal blood flow–metabolism uncoupling in the Cu/Zn‐superoxide dismutase 1 (SOD1)‐transgenic (Tg) mouse model of amyotrophic lateral sclerosis (ALS), suggesting relative hypoxia in the spinal cord. However, the hypoxic stress sensor pathway in ALS has not been well studied. In the present work, we examined the temporal and spatial changes of hypoxic stress sensor proteins (Siah‐1, PHD3, and FIH) following motor neuron (MN) degeneration in the spinal cord of normoxic ALS mice. The expression of Siah‐1 and PHD3 proteins progressively increased in the surrounding glial cells of presymptomatic Tg mice (10 weeks, 10 weeks) compared with the large MN of the anterior horn. In contrast, a significant reduction in Siah‐1 and PHD3 protein expression was evident in end‐stage ALS mice (18 weeks, 18 weeks). Double‐immunofluorescence analysis revealed PHD3 plus Siah‐1 double‐positive cells in the surrounding glia of symptomatic Tg mice (14–18 weeks), with no change in the large MNs. In contrast, FIH protein expression decreased in the surrounding glial cells of Tg mice at end‐stage ALS (18 weeks). The present study suggests a partial loss in the neuroprotective response of spinal MNs in ALS results from a relative hypoxia through the Siah‐1, PHD3, and FIH system under normoxic conditions. This response could be an important mechanism of neurodegeneration in ALS. © 2012 Wiley Periodicals, Inc.
N‐methyl‐D‐aspartate receptor‐mediated axonal injury in adult rat corpus callosum
Authors: Zhang Jingdong , Liu Jianuo , Fox Howard S. , Xiong Huangui 
Published: November 2012

AbstractDamage to white matter such as corpus callosum (CC) is a pathological characteristic in many brain disorders. Glutamate (Glut) excitotoxicity through AMPA receptors on oligodendrocyte (OL) was previously considered as a mechanism for white matter damage. Recent studies have shown that N‐methyl‐D‐aspartate receptors (NMDARs) are expressed on myelin sheath of neonatal rat OL processes and that activation of these receptors mediated demyelization. Whether NMDARs are expressed in the adult CC and are involved in excitotoxic axonal injury remains to be determined. In this study, we demonstrate the presence of NMDARs in the adult rat CC and their distributions in myelinated nerve fibers and OL somata by means of immunocytochemical staining and Western blot. Incubation of the CC slices with Glut or NMDA induced axonal injury as revealed by analyzing amplitude of CC fiber compound action potentials (CAPs) and input–output response. Both Glut and NMDA decreased the CAP amplitude and input–output responses, suggesting an involvement of NMDARs in Glut‐ and NMDA‐induced axonal injury. The involvement of NMDAR in Glut‐induced axonal injury was further assayed by detection of β‐amyloid precursor protein (β‐APP) in the CC axonal fibers. Treatment of the CC slices with Glut resulted in β‐APP accumulation in the CC fibers as detected by Western blot, reflecting an impairment of axonal transport function. This injurious effect of Glut on CC axonal transport was significantly blocked by MK801. Taken together, these results show that NMDARs are expressed in the adult CC and are involved in excitotoxic activity in adult CC slices in vitro. © 2012 Wiley Periodicals, Inc.
Hyperexcitability of inferior colliculus neurons caused by acute noise exposure
Authors: Niu Yuguang , Kumaraguru Anand , Wang Rongguang , Sun Wei 
Published: November 2012

AbstractNoise exposure is one of the most common causes of hearing loss. Recent studies found that noise exposure‐induced cochlear damage may change the excitability and tonotopic organization of the central auditory system (CAS). This plasticity was suspected to be related to tinnitus and hyperacusis. However, how cochlear damage affects CAS function and causes these neurologic diseases is still not clear. CAS function is activity dependent, so we hypothesize that a restricted cochlear lesion might disrupt the balance of excitation and inhibition in the CAS and thereby affect its neural activity. To test this hypothesis, we studied the effects of narrow‐band noise exposure on the firing properties of neurons in the inferior colliculus (IC), which has complex neural circuits and plays an important role in sound processing. We found that noise exposure (20 kHz, 105 dB SPL, 30 min) caused a dramatic decrease of the characteristic frequency in about two‐thirds of high‐frequency neurons with/without causing a significant threshold shift. The noise exposure also caused an increase in firing rate of the low‐frequency neurons at suprathreshold levels, whereas it dramatically decreased the firing rate of the high‐frequency neurons. Our results suggest that acute high‐frequency noise exposure may increase low‐frequency responsiveness by causing hyperexcitability of low‐frequency neurons. The functional change of the low‐frequency neurons may be related to the disruption of side‐band inhibition at the noise exposure frequencies caused by cochlear damage. © 2012 Wiley Periodicals, Inc.
Optical inhibition of motor nerve and muscle activity in vivo
Authors: Liske Holly , Towne Chris , Anikeeva Polina , Zhao Shengli , Feng Guoping , Deisseroth Karl , Delp Scott 
Published: November 2012

AbstractIntroduction: There is no therapeutic approach that provides precise and rapidly reversible inhibition of motor nerve and muscle activity for treatment of spastic hypertonia. Methods: We used optogenetics to demonstrate precise and rapidly reversible light‐mediated inhibition of motor nerve and muscle activity in vivo in transgenic Thy1::eNpHR2.0 mice. Results: We found optical inhibition of motor nerve and muscle activity to be effective at all muscle force amplitudes and determined that muscle activity can be modulated by changing light pulse duration and light power density. Conclusions: This demonstration of optical inhibition of motor nerves is an important advancement toward novel optogenetics‐based therapies for spastic hypertonia. Muscle Nerve 47: 916–921, 2013
Prophylactic administration of prothrombin complex concentrates for congenital prothrombin deficiency with a novel frameshift mutation, prothrombin saitama
Authors: Seki Masafumi , Koh Katsuyoshi , Inoue Takeshi , Tomita Yuzo , Kato Motohiro , Shimizu Masaki , Morishita Eriko , Hanada Ryoji 
Published: November 2012

AbstractProthrombin (Factor II, FII) deficiency is an extremely rare autosomal recessive condition with an estimated incidence of 1:2 million. As severe and life‐threatening bleeding is rare in FII deficiency, on demand therapy with administration of prothrombin complex concentrates (PCCs) or fresh frozen plasma is generally performed, and prophylactic therapy for FII deficiency has been reported in only three cases. Thus, its optimal dosage and schedule has remained uncertain. Here we report a case of severe prothrombin deficiency with a novel frameshift mutation of the F2 gene, who was started on prophylactic administration. Pediatr Blood Cancer 2013; 60: 503–505. © 2012 Wiley Periodicals, Inc.
SILAC‐based quantitative proteomic analysis of gastric cancer secretome
Authors: Marimuthu Arivusudar , Subbannayya Yashwanth , Sahasrabuddhe Nandini A. , Balakrishnan Lavanya , Syed Nazia , Sekhar Nirujogi Raja , Katte Teesta V. , Pinto Sneha M. , Srikanth Srinivas M. , Kumar Praveen , Pawar Harsh , Kashyap Manoj K. , Maharudraiah Jagadeesha , Ashktorab Hassan , Smoot Duane T. , Ramaswamy Girija , Kumar Rekha V. , Cheng Yulan , Meltzer Stephen J. , Roa Juan Carlos , Chaerkady Raghothama , Prasad T. S. Keshava , Harsha H. C. , Chatterjee Aditi , Pandey Akhilesh 
Published: November 2012

PurposeGastric cancer is a commonly occurring cancer in Asia and one of the leading causes of cancer deaths. However, there is no reliable blood‐based screening test for this cancer. Identifying proteins secreted from tumor cells could lead to the discovery of clinically useful biomarkers for early detection of gastric cancer.Experimental designA SILAC‐based quantitative proteomic approach was employed to identify secreted proteins that were differentially expressed between neoplastic and non‐neoplastic gastric epithelial cells. Proteins from the secretome were subjected to SDS‐PAGE and SCX‐based fractionation, followed by mass spectrometric analysis on an LTQ‐Orbitrap Velos mass spectrometer. Immunohistochemical labeling was employed to validate a subset of candidates using tissue microarrays.ResultsWe identified 2205 proteins in the gastric cancer secretome of which 263 proteins were overexpressed greater than fourfold in gastric cancer‐derived cell lines as compared to non‐neoplastic gastric epithelial cells. Three candidate proteins, proprotein convertase subtilisin/kexin type 9 (PCSK9), lectin mannose binding 2 (LMAN2), and PDGFA‐associated protein 1 (PDAP1) were validated by immunohistochemical labeling.Conclusions and clinical relevanceWe report here the largest cancer secretome described to date. The novel biomarkers identified in the current study are excellent candidates for further testing as early detection biomarkers for gastric adenocarcinoma.
Electron–phonon coupling in the two‐dimensional electron gas on Bi2Se3
Authors: Barreto Lucas , Bianchi Marco , Guan Dandan , Hatch Richard , Mi Jianli , Iversen Bo Brummerstedt , Hofmann Philip 
Published: November 2012

Abstractmagnified imageThe electron–phonon coupling in the Rashba‐split two‐dimensional electron gas near the surface of the topological insulator Bi2Se3 is studied by temperature‐dependent angleresolved photoemission. The coupling strength is found to be rather weak, with an electron–phonon mass enhancement parameter $ \lambda = 0.12(8). $ (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Hydrogen isotope effects on covalent and noncovalent interactions: The case of protonated rare gas clusters
Authors: Moncada Félix , Uribe Lalita S. , Romero Jonathan , Reyes Andrés 
Published: November 2012

AbstractWe investigate hydrogen isotope and nuclear quantum effects on geometries and binding energies of small protonated rare gas clusters (Rg
$_n$X
$^ +$, Rg = He,Ne,Ar, X = H,D,T, and
$n$ = 1–3) with the any particle molecular orbital (APMO) MP2 level of theory (APMO/MP2). To gain insight on the impact of nuclear quantum effects on the different interactions present in the Rg
$_n$X
$^ +$ systems, we propose an APMO/MP2 energy decomposition analysis scheme. For RgH
$^ +$ ions, isotopic substitution leads to an increase in the stability of the complex, because polarization and charge transfer contributions increase with the mass of the hydrogen. In the case of Rg
$_2$H
$^ +$ complexes, isotopic substitution results in a shortening and weakening of the rare gas‐hydrogen ion bond. For Rg
$_3$X
$^ +$ complexes, the isotope effects on the rare gas binding energy are almost negligible. Nevertheless, our results reveal that subtle changes in the charge distribution of the Rg
$_2$X
$^ +$ core induced by an isotopic substitution have an impact on the geometry of the Rg
$_3$X
$^ +$ complex. © 2012 Wiley Periodicals, Inc.
Curracurrong: a stream programming environment for wireless sensor networks
Authors: Kakkad Vasvi , Attar Saeed , Santosa Andrew E. , Fekete Alan , Scholz Bernhard 
Published: November 2012

SUMMARYThe technological advances in wireless sensor network (WSN) enable the development of complex applications including health monitoring, environmental sampling, and disaster area monitoring. WSN applications deploy battery‐powered sensors at remote locations for long periods. The development of energy‐efficient and complex WSN applications therefore requires in‐depth embedded systems programming skills that are normally not found in domain experts. So that this challenge can be overcome, programming environments for WSN need to offer a high degree of productivity, flexibility, and efficiency at the same time. In this work, we present Curracurrong, a development environment for WSNs that is based on expressing queries with stream programming. A query is represented as a stream graph consisting of stream operators and communication channels. Curracurrong provides an extensible stream operator library that adapts to a wide range of applications. It uses a novel placement algorithm that optimizes the energy consumption on sensor nodes. Through a case study, we demonstrate the productivity and flexibility of our system. We conduct experiments that evaluate the energy efficiency of our optimized operator placement algorithm. Copyright © 2012 John Wiley & Sons, Ltd.