bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of histone deacetylase 4 increases cytotoxicity of docetaxel in gastric cancer cells
Authors: Colarossi Lorenzo , Memeo Lorenzo , Colarossi Cristina , Aiello Eleonora , Iuppa Antonio , Espina Virginia , Liotta Lance , Mueller Claudius 
Published: August 2014

PurposeNew treatment options for gastric cancer are in great demand. Histone deacetylases (HDACs) are exciting therapeutic targets, but only the class I HDACs 1, 2, and 3 have been studied in gastric cancer. We have investigated class IIa HDAC expression and inhibition in gastric cancer cells.Experimental designWe measured the level of 27 (phospho)proteins related to class IIa HDAC expression and function in ten laser‐capture microdissection gastric tumor samples compared to patient‐matched adjacent normal mucosa. Following, we evaluated class IIa HDAC inhibition by MC1568 in SNU‐16 gastric cancer cells alone and in combination with cisplatin or docetaxel.ResultsWe demonstrate for the first time an increase of HDAC4 in gastric tumor cells. HDAC4 inhibition had a synergistic effect with docetaxel treatment, shifting the cellular response from a cytostatic to a cytotoxic phenotype. This effect was associated with increased levels of cleaved caspases 3 and 9 and increased acetylated histone H3 Lys9/Lys14.Conclusions and clinical relevanceThese data support in vivo studies investigating the potential clinical use of HDAC4 inhibitors in combination with docetaxel for the treatment of gastric cancer, lowering treatment doses of docetaxel to reduce the burden of adverse side effects on patients.
Flanged foundation connection of the Offshore Wind Farm Amrumbank West – Concept, approval, design, tests and installation
Authors: Gollub Peter , Jensen Jacob Fisker , Giese Dirk , Güres Selcuk 
Published: August 2014

AbstractMonopile (MP) foundations are the preferred foundation concept for offshore wind turbine generators (WTG) with soil conditions suitable for driving. Transition Pieces (TP’s) are required for the connection of the driven MP to the tower of the wind turbine. The most commonly used connection between the MP and the TP is grouting of the annulus between MP and TP with fine grained high durability concrete, so called grouted joint. In the Amrumbank wind farm project a flanged connection is used. In this paper the concept of the flanged joint, main aspects of approval, design, procedure tests, manufacturing, prototype assembly and installation are described.Fundamentflanschverbindung der Offshore Wind Farm Amrumbank West − Konzept, Genehmigung, Design, Tests und Montage. Die Gründung mit Monopiles (MP) ist bei geeigneten Baugrundbedingungen häufig das bevorzugte Konzept für die Gründung von Offshore Windenergieanlagen (WEA). Transition Pieces (TP, Übergangsstücke) werden zur Verbindung des gerammten MP zum Turm der WEA benötigt. Die herkömmliche Verbindung zwischen MP und TP ist der Verguss mit Feinkornbeton. Für das Amrumbank‐Projekt kommt eine Ringflanschverbindung als MP‐TP‐Verbindung zur Ausführung. Das Konzept der Flanschverbindung, wesentliche Gesichtspunkte zur Genehmigung, Design, Verfahrenstests, Fertigung, Prototype‐Montage und Installation werden im folgenden Beitrag beschrieben.
Planung, Genehmigung und Ausführung des Offshore‐Windparks Amrumbank West
Authors: Schwegmann Dominik , Kossel Thomas , Göhlmann Joachim , Lochte‐Holtgreven Stephan 
Published: August 2014

AbstractDer Offshore‐Windpark Amrumbank West liegt 35 km nördlich der Insel Helgoland und 35 km westlich der Insel Amrum in der Ausschließlichen Wirtschaftzone Deutschlands in der Nordsee und befindet sich derzeit in der Realisierungsphase. Nach mehr als dreijähriger Planungs‐ und Genehmigungsphase berichtet dieser Beitrag über die Planung und Genehmigung des Offshore‐Windparks. Neben den für die 80 Monopile‐Tragstrukturen in knapp 25 m Wassertiefe maßgebenden und bereits in der statischen Vorplanungsphase berücksichtigten Beanspruchungen werden Besonderheiten in der Genehmigungsphase beschrieben und die erforderlichen Stahltonnagen für die Projektumsetzung vorgestellt. Abschließend werden erste Meilensteine in der Projektrealisierung erläutert und ein Überblick über die ausgeführten Sonderlösungen gegeben.Planning, certification and installation of Amrumbank West offshore wind farm. The offshore wind farm Amrumbank West is located 35 km in the north of Helgoland and 35 km kilometers in the west of the island of Amrum in the exclusive economic zone of Germany. The installation of Amrumbank West is currently ongoing. After more than three years of development and certification, this paper deals with the main topics of planning and certification of the offshore wind farm. Besides the design driving parameters which have been already considered during the conceptual design phase of the 80 monopile substructures in nearly 25 m water depths, specific features of the certification process will be discussed. Furthermore, first milestones of the installation and special technical solutions are presented.
The Center of Biological Nitrogen Fixation: FeMo‐Cofactor
Authors: Spatzal Thomas 
Published: August 2014

AbstractThe enzyme nitrogenase is the only known biological system capable of reducing atmospheric dinitrogen to ammonia, and is therefore an essential player in the biological nitrogen cycle. Despite decades of research, essential questions about the mechanism of nitrogen fixation are unanswered. The complexity of the nitrogenase active site FeMo‐cofactor contributes thereby significantly to the problems nitrogenase research is facing today. Attempts to mimic its exceptional catalytic properties were without success and also the metal center itself exposed only a small fraction of its secrets so far. Today, the FeMo‐cofactor is still the largest and most complex metal center known in biological systems. Solely its structure determination turned out to be an unexpected long process facing multiple hurdles. Even though X‐ray crystallography led to first structural data of the [7Fe:9S:C:Mo]:R‐homocitrate entity more than twenty years ago, only recently the entire atomic structure of the metal center was revealed. This review gives a short overview of the FeMo‐cofactor with a special focus on its structure determination.
A New Method of Preparing Copper Oxide from Dinitrourea Copper Salt
Authors: Il'yasov Sergey G. , Kazantsev Igor V. , Til'zo Mikhail V. , Sakovich Gennady V. , Zaikovskii Vladimir I. , Prosvirin Igor P. , Tuzikov Fedor V. 
Published: August 2014

AbstractA new approach for the preparation of copper nanooxide by solvothermal decomposition of N, N′‐dinitrourea copper(II) salt in an aprotic solvent was developed. The resulting particles were studied by transmission electron microscopy, X‐ray photoelectron spectroscopy, and small‐angle X‐ray scattering. The effects of different solvents on shapes of the agglomerates formed are shown.
The High‐Pressure Borate HP‐(NH4)B3O5
Authors: Sohr Gerhard , Wilhelm Dominik , Vitzthum Daniela , Schmitt Martin K. , Huppertz Hubert 
Published: August 2014

AbstractThe high‐pressure ammonium triborate HP‐(NH4)B3O5 was synthesized in a high‐pressure/high‐temperature experiment at 3 GPa and 600 °C. It crystalizes in the monoclinic space group C2/c (No. 15) with the parameters a = 981.0(2), b = 880.2(2), c = 922.2(2) pm, β = 103.8(1)°, V = 0.7735(3) nm3, Z = 8, R1 = 0.0436, and wR2 = 0.1059 (all data). The compound is built up from ribbons of interconnected corner‐ and edge‐sharing BO4 tetrahedra that are linked by planar BO3 groups to form a three‐dimensional structure. The ammonium ions are situated in channels, forming four hydrogen bonds to oxygen of the boron oxygen network. The structure is a substitutional variant of the compounds HP‐MB3O5, (M = K, Rb) and HP‐Cs1–x(H3O)xB3O5 (x = 0.5–0.7). The collected IR and Raman spectra of HP‐(NH4)B3O5 show great similarities to the spectra of the analogous phases.
Pseudomelanosis duodeni in a female adult with chronic renal failure
Authors: Sun Wei‐Chih , Tsay Feng‐Woei , Lai Kwok‐Hung , Hsu Ping‐I , Shih Chih‐An 
Published: August 2014

SummaryPseudomelanosis duodeni is a rare endoscopic finding that manifests as dark speckled spots in the duodenum. It is considered a benign condition and is associated with certain diseases and the use of certain medications. This study reports a case of a 74‐year‐old woman, with end‐stage renal disease under maintenance hemodialysis, hypertension under regular medical control, iron deficiency anemia under oral iron supplement, and progressive anemia with suspicious occult gastrointestinal bleeding. Upper gastrointestinal endoscopy revealed multiple tiny brownish‐black pigmentation throughout the proximal second portion of the duodenum. The histopathological examination showed pigment‐laden macrophages with positive iron stain and negative melanin stain in the lamina propria of the mucosal villi.
Aubry–Mather theory for homeomorphisms
Authors: FATHI ALBERT , PAGEAULT PIERRE 
Published: August 2014

AbstractIn this paper, we develop a variational approach to study the dynamic of a homeomorphism on a compact metric space. In particular, we describe orbits along which any Lipschitz Lyapunov function has to be constant via a non-negative Lipschitz semi-distance. We give the link with Auslander’s notion of generalized recurrence, and recover in a different way some parts of a more recent work of Akin and Auslander.
Comparative single-cell genomics reveals potential ecological niches for the freshwater acI Actinobacteria lineage
Authors: Ghylin Trevor W , Garcia Sarahi L , Moya Francisco , Oyserman Ben O , Schwientek Patrick , Forest Katrina T , Mutschler James , Dwulit-Smith Jeffrey , Chan Leong-Keat , Martinez-Garcia Manuel , Sczyrba Alexander , Stepanauskas Ramunas , Grossart Hans-Peter , Woyke Tanja , Warnecke Falk , Malmstrom Rex , Bertilsson Stefan , McMahon Katherine D 
Published: August 2014

Abstract
Members of the acI lineage of Actinobacteria are the most abundant microorganisms in most freshwater lakes; however, our understanding of the keys to their success and their role in carbon and nutrient cycling in freshwater systems has been hampered by the lack of pure cultures and genomes. We obtained draft genome assemblies from 11 single cells representing three acI tribes (acI-A1, acI-A7, acI-B1) from four temperate lakes in the United States and Europe. Comparative analysis of acI SAGs and other available freshwater bacterial genomes showed that acI has more gene content directed toward carbohydrate acquisition as compared to Polynucleobacter and LD12 Alphaproteobacteria, which seem to specialize more on carboxylic acids. The acI genomes contain actinorhodopsin as well as some genes involved in anaplerotic carbon fixation indicating the capacity to supplement their known heterotrophic lifestyle. Genome-level differences between the acI-A and acI-B clades suggest specialization at the clade level for carbon substrate acquisition. Overall, the acI genomes appear to be highly streamlined versions of Actinobacteria that include some genes allowing it to take advantage of sunlight and N-rich organic compounds such as polyamines, di- and oligopeptides, branched-chain amino acids and cyanophycin. This work significantly expands the known metabolic potential of the cosmopolitan freshwater acI lineage and its ecological and genetic traits.
Depth-stratified functional and taxonomic niche specialization in the ‘core’ and ‘flexible’ Pacific Ocean Virome
Authors: Hurwitz Bonnie L , Brum Jennifer R , Sullivan Matthew B 
Published: August 2014

Abstract
Microbes drive myriad ecosystem processes, and their viruses modulate microbial-driven processes through mortality, horizontal gene transfer, and metabolic reprogramming by viral-encoded auxiliary metabolic genes (AMGs). However, our knowledge of viral roles in the oceans is primarily limited to surface waters. Here we assess the depth distribution of protein clusters (PCs) in the first large-scale quantitative viral metagenomic data set that spans much of the pelagic depth continuum (the Pacific Ocean Virome; POV). This established ‘core’ (180 PCs; one-third new to science) and ‘flexible’ (423K PCs) community gene sets, including niche-defining genes in the latter (385 and 170 PCs are exclusive and core to the photic and aphotic zones, respectively). Taxonomic annotation suggested that tailed phages are ubiquitous, but not abundant (&lt;5% of PCs) and revealed depth-related taxonomic patterns. Functional annotation, coupled with extensive analyses to document non-viral DNA contamination, uncovered 32 new AMGs (9 core, 20 photic and 3 aphotic) that introduce ways in which viruses manipulate infected host metabolism, and parallel depth-stratified host adaptations (for example, photic zone genes for iron–sulphur cluster modulation for phage production, and aphotic zone genes for high-pressure deep-sea survival). Finally, significant vertical flux of photic zone viruses to the deep sea was detected, which is critical for interpreting depth-related patterns in nature. Beyond the ecological advances outlined here, this catalog of viral core, flexible and niche-defining genes provides a resource for future investigation into the organization, function and evolution of microbial molecular networks to mechanistically understand and model viral roles in the biosphere.
Self-Reported Spatial Hearing Abilities Across Different Cochlear Implant Profiles
Authors: Perreau Ann E. , Ou Hua , Tyler Richard , Dunn Camille 
Published: August 2014

Purpose
The goal of this study was to determine how self-reported spatial hearing abilities differ across various cochlear implant (CI) profiles and to examine the degree of subjective benefit following cochlear implantation across different groups of CI users.

Method
This was a retrospective study of subjective spatial hearing ability of CI recipients. The subjects consisted of 99 unilateral CI users, 49 bilateral CI users, 32 subjects with a CI and contralateral hearing aid (bimodal users), and 37 short-electrode CI users. All subjects completed the Spatial Hearing Questionnaire (Tyler, Perreau, & Ji, 2009), a questionnaire assessing spatial hearing ability, after implantation, and a subset of the subjects completed the questionnaire pre- and postimplantation.

Results
Subjective spatial hearing ability was rated higher for the bilateral and short electrode CI users compared to the unilateral and bimodal users. There was no significant difference in subjective spatial hearing performance between the bilateral and short electrode CI users and the unilateral CI and bimodal users. A separate analysis of pre- and postimplant performance revealed that all CI groups reported significant improvements in spatial hearing ability after implantation.

Conclusion
This study suggests that there are substantial differences in perceived spatial hearing ability among unilateral and bimodal CI users compared with bilateral and short electrode CI users.
Team Collaboration: The Use of Behavior Principles for Serving Students With ASD
Authors: Donaldson Amy L. , Stahmer Aubyn C. 
Published: August 2014

Purpose
Speech-language pathologists (SLPs) and behavior analysts are key members of school-based teams that serve children with autism spectrum disorders (ASD). Behavior analysts approach assessment and intervention through the lens of applied behavior analysis (ABA). ABA-based interventions have been found effective for targeting skills across multiple domains for children with ASD. However, some SLPs may be unfamiliar with the breadth of ABA-based interventions. The intent of this tutorial is to briefly introduce key ABA principles, provide examples of ABA-based interventions used within schools, and identify strategies for successful collaboration between behavior analysts and SLPs.

Method
This tutorial draws from empirical studies of ABA-based interventions for children with ASD within school settings, as well as discussions in the extant literature about the use of behavior principles by SLPs and strategies for interdisciplinary collaboration.

Conclusion
Given the prevalence of ASD at 1 in 68 children (Centers for Disease Control and Prevention, 2014) and the high cost of serving these children within schools (an average cost of 286% over regular education; Chambers, Shkolnik, & Perez, 2003), the need for effective, comprehensive service provision and efficiency within interdisciplinary teams is paramount. Communication, mutual understanding, and recognition of common ground between SLPs and behavior analysts can lead to successful collaboration.
Antibody-based delivery of IL4 to the neovasculature cures mice with arthritis
Authors: Hemmerle Teresa , Doll Fabia , Neri Dario 
Published: August 2014

SignificanceDisease-homing antibody–cytokine fusion proteins (immunocytokines) are considered as innovative biopharmaceutical agents for the therapy of cancer and chronic inflammatory conditions with the potential to modulate the activity of the immune system at the site of disease. The immunocytokine F8-IL4 was able to selectively localize to arthritic sites in vivo and exhibited a potent single-agent activity in the collagen-induced arthritis model in mice. Surprisingly, the combination treatment of F8-IL4 with dexamethasone cured 100% of treated mice with established arthritis. To our knowledge, this is the first report of durable and complete regressions in mice with established RA. These findings are of clinical significance as the F8 antibody recognizes its cognate antigen, the extra domain A of fibronectin, with comparable affinity in mouse and man.
Variable cooperativity in SNARE-mediated membrane fusion
Authors: Hernandez Javier M. , Kreutzberger Alex J. B. , Kiessling Volker , Tamm Lukas K. , Jahn Reinhard 
Published: August 2014

Significance
The merging of lipid bilayer membranes, or membrane fusion, is a ubiquitous process in cellular trafficking in eukaryotic cells. The responsible proteins have long been known to be the soluble N-ethylmaleimide–sensitive factor attachment protein receptors (SNAREs); however, key mechanistic details regarding how they work remain elusive. Among them is the issue of cooperativity, which asks how many SNAREs are needed for fusion to take place. Hitherto, reports have addressed this question in terms of fixed numbers, providing a rather static picture of how SNAREs operate. Using an elaborate kinetic analysis, we provide strong biochemical evidence showing that cooperativity is highly variable and will depend on the energy barrier of the membrane fusion reaction in question, implying SNAREs are much more modular and dynamic than previously thought.
Neuraminidase-Inhibiting Antibody Response to H5N1 Virus Vaccination in Chronically Ill and Immunocompromised Patients
Authors: Fritz Richard , Hetzelt Nicole , Ilk Reinhard , Hohenadl Christine , van der Velden Maikel V. W. , Aichinger Gerald , Portsmouth Daniel , Kistner Otfried , Howard M. Keith , Barrett P. Noel , Kreil Thomas R. 
Published: August 2014

Abstract
Neuraminidase-inhibiting (NAi) antibodies have been reported to be an independent correlate of protection from influenza disease, but the NAi antibody response to influenza vaccination has never been assessed in chronically ill or immunocompromised participants. Using an enzyme-linked lectin assay, we demonstrated that 2 immunizations with a Vero cell culture-derived, whole-virus H5N1 A/Vietnam vaccine induces NAi antibodies in 94.3% of chronically ill and 83.8% of immunocompromised participants. A booster with a heterologous A/Indonesia H5N1 vaccine induced comparable NAi antibody titers in both groups and resulted in 100% seropositivity. These data support prepandemic H5N1 vaccination strategies for these highly vulnerable risk groups.
Study of negative and positive superhumps in ER Ursae Majoris
Authors: Ohshima Tomohito , Kato Taichi , Pavlenko Elena , Akazawa Hidehiko , Imamura Kazuyoshi , Tanabe Kenji , de Miguel Enrique , Stein William , Itoh Hiroshi , Hambsch Franz-Josef , Dubovsky Pavol A. , Kudzej Igor , Krajci Thomas , Baklanov Alex , Samsonov Denis , Antonyuk Oksana I. , Malanushenko Viktor , Andreev Maksim V. , Noguchi Ryo , Ogura Kazuyuki , Nomoto Takashi , Ono Rikako , Nakagawa Shin’ichi , Taniuchi Keisuke , Aoki Tomoya , Kawabata Miho , Kimura Hitoshi , Masumoto Kazunari , Kobayashi Hiroshi , Matsumoto Katsura , Shiokawa Kazuhiko , Shugarov Sergey Yu. , Katysheva Natalia , Voloshina Irina , Zemko Polina , Kasai Kiyoshi , Ruiz Javier , Maehara Hiroyuki , Virnina Natalia , Virtanen Jani , Miller Ian , Boitnott Boyd , Littlefield Colin , James Nick , Tordai Tamas , Robert Fidrich , Padovan Stefano , Miyashita Atsushi 
Published: August 2014

AbstractWe carried out photometric observations of the SU UMa-type dwarf nova ER UMa during 2011 and 2012, which showed the existence of persistent negative superhumps even during the superoutburst. We performed a two-dimensional period analysis of its light curves by using a method called “least absolute shrinkage and selection operator” (Lasso) and the “phase dispersion minimization” (PDM) analysis, and found that the period of negative superhumps systematically changed between a superoutburst and the next superoutburst. The trend of the period change can be interpreted as a reflection of the change of the disk radius. This change is in agreement with the one predicted by the thermal tidal instability model. The normal outburst during a supercycle showed a general trend that the rising rate to its maximum becomes slower as the next superoutburst is approaching. The change can be interpreted as the consequence of the increased gas-stream flow into the inner region of the disk as a result of the tilted disk. Some of superoutbursts were found to be triggered by a precursor normal outburst when the positive superhump appeared to develop. The positive and negative superhumps coexisted during the superoutburst. Positive superhumps were prominent only for four or five days after the supermaximum, while the signal of negative superhumps became stronger after the middle phase of the superoutburst plateau. A simple combination of the positive and negative superhumps was found to be insufficient for reproduction of the complex profile variation. We were able to detect the developing phase of positive superhumps (stage A superhumps) for the first time in ER UMa-type dwarf novae. Using the period of stage A superhumps, we obtained a mass ratio of 0.100(15), which indicates that ER UMa is on the ordinary evolutional track of cataclysmic variable stars.
Public Life as Tragedy
Authors: FRENCH RICHARD D. 
Published: August 2014

AbstractSome of the most perceptive observers of public life have emphasised its tragic dimensions, not so much out of sympathy for politicians, but because the lens of tragedy offers a unique insight into the realities of the world of politics. Here I attempt to synthesise this tragic perspective by employing the comments of those best positioned to identify the salient features of public life, its primary dramatis personae. Politics occasionally provides us with the kind of spectacular catastrophe that journalists like to construe as tragedy. But our purpose is to evoke a different, more personal, less visible kind of tragedy: the small but malignant tragedies of self‐betrayal, of inflation of the ego and deflation of conscience, of helpless witness to injustice and misfortune, of status unaccompanied by power or efficacy, of the shrinking of aspiration to the scale of the practicable, of disillusion and, on occasion, of despair.