bluuebunny/crossref_metadata_2025_split huggingface dataset:
Classification of African ground pangolin behaviour based on accelerometer readouts: validation of bio-logging methods
Authors: Harvey-Carroll Jessica , Carroll Daire , Trivella Cara-Marie , Connelly Ellen 
Published: July 2024

Abstract
Background
Understanding how free-ranging animals behave can help in the design of optimal management strategies to both conserve species and enable individuals to express natural behaviours, maximising welfare. Animal-borne accelerometers passively collect data on body acceleration which can be interpreted to quantify behaviour. Accelerometers are increasingly used in behavioural research, however validation of accelerometer readouts to enable classification of discrete behaviours is required for each species. Pangolins are a heavily trafficked clade of mammals, all of which are considered vulnerable to extinction. They are also under-researched, with little known about their behaviour in the wild. In this study, we present the first validation of behavioural classification based on accelerometer readouts for a pangolin species; the ground pangolin (Smutsia temminckii).

Results
We present a standardised protocol for attaching accelerometers to pangolins to minimise the impact of devices on welfare. We match the readouts from accelerometers to behaviours defined through video observations. Using a random forest classification, we defined five discrete behaviours (walking, digging, feeding, investigating ground, and stationary, accuracy of 85%) and three activity levels (low, medium, and high, accuracy of 94%) from accelerometer readouts. We determine optimal sampling frequency and window length (50 Hz and five seconds for discrete behaviour, 10 Hz and seven seconds for activity level). We then deploy accelerometers and classify the behaviour of three free-ranging pangolins for between two and four days. We find considerable variation in peak daily activity between free-ranging pangolins with different individuals displaying nocturnal and crepuscular behaviour. We also find that pangolins spend the majority of their time (between 62 and 71%) at rest.

Conclusion
The methods we present will enable the quantification of ground pangolin behaviour in the wild to improve our understanding of the species’ ecology and help inform conservation efforts. This will also help to improve our fundamental understanding of animal behaviour and ecology.
TGF-β1 mediates hypoxia-preconditioned olfactory mucosa mesenchymal stem cells improved neural functional recovery in Parkinson’s disease models and patients
Authors: Zhuo Yi , Li Wen-Shui , Lu Wen , Li Xuan , Ge Li-Te , Huang Yan , Gao Qing-Tao , Deng Yu-Jia , Jiang Xin-Chen , Lan Zi-Wei , Deng Que , Chen Yong-Heng , Xiao Yi , Lu Shuo , Jiang Feng , Liu Zuo , Hu Li , Liu Yu , Ding Yu , He Zheng-Wen , Tan De-An , Duan Da , Lu Ming 
Published: July 2024

Abstract
Background
Parkinson’s disease (PD) is a neurodegenerative disorder characterized by the degeneration of dopaminergic neurons in the substantia nigra (SN). Activation of the neuroinflammatory response has a pivotal role in PD. Mesenchymal stem cells (MSCs) have emerged as a promising therapeutic approach for various nerve injuries, but there are limited reports on their use in PD and the underlying mechanisms remain unclear.

Methods
We investigated the effects of clinical-grade hypoxia-preconditioned olfactory mucosa (hOM)-MSCs on neural functional recovery in both PD models and patients, as well as the preventive effects on mouse models of PD. To assess improvement in neuroinflammatory response and neural functional recovery induced by hOM-MSCs exposure, we employed single-cell RNA sequencing (scRNA-seq), assay for transposase accessible chromatin with high-throughput sequencing (ATAC-seq) combined with full-length transcriptome isoform-sequencing (ISO-seq), and functional assay. Furthermore, we present the findings from an initial cohort of patients enrolled in a phase I first-in-human clinical trial evaluating the safety and efficacy of intraspinal transplantation of hOM-MSC transplantation into severe PD patients.

Results
A functional assay identified that transforming growth factor-β1 (TGF-β1), secreted from hOM-MSCs, played a critical role in modulating mitochondrial function recovery in dopaminergic neurons. This effect was achieved through improving microglia immune regulation and autophagy homeostasis in the SN, which are closely associated with neuroinflammatory responses. Mechanistically, exposure to hOM-MSCs led to an improvement in neuroinflammation and neural function recovery partially mediated by TGF-β1 via activation of the anaplastic lymphoma kinase/phosphatidylinositol-3-kinase/protein kinase B (ALK/PI3K/Akt) signaling pathway in microglia located in the SN of PD patients. Furthermore, intraspinal transplantation of hOM-MSCs improved the recovery of neurologic function and regulated the neuroinflammatory response without any adverse reactions observed in patients with PD.

Conclusions
These findings provide compelling evidence for the involvement of TGF-β1 in mediating the beneficial effects of hOM-MSCs on neural functional recovery in PD. Treatment and prevention of hOM-MSCs could be a promising and effective neuroprotective strategy for PD. Additionally, TGF-β1 may be used alone or combined with hOM-MSCs therapy for treating PD.
Efficacy of tracheostomy for respiratory management in patients with advanced oral cancer
Authors: Kim Yun-Ho , Yang Jae-Young , Ma Yoon-Hee , Lee Jin-Choon , Hwang Dae-Seok , Ryu Mi-Heon , Kim Uk-Kyu 
Published: July 2024

Abstract
Background
Many studies have been reported on tracheostomy to prevent upper airway obstruction after surgery. Among these, the scoring system proposed by Cameron et al. quantifies various factors that influence postoperative respiratory failure. This system provides a basis for surgeons to decide whether to perform an elective tracheostomy. In this study, the authors applied the Cameron scoring system retrospectively to patients undergoing severe oral cancer surgery to reevaluate the indications for elective tracheostomy and to investigate its clinical efficacy in airway management. In this study, a sample of 20 patients who underwent oral cancer surgery was selected and divided into two groups: 10 underwent tracheostomy and 10 did not. The Cameron scoring scores for each patient were extracted, to verify whether elective tracheostomy was performed in accordance with the threshold scores. Differences in scores and significant clinical impact factors between the two groups were analyzed and compared.

Result
The 10 patients who underwent tracheostomy had an average Cameron score of 6.4, all scoring above the recommended threshold of 5 for tracheostomy. For the 10 patients who did not undergo tracheostomy, the average score was 2.5, with 8 out of these 10 patients scoring below 5. Significant clinical impact factors observed included the location and size of the tumor, the performance of mandibulectomy and neck dissection, and the type of reconstruction surgery.

Conclusion
In planning surgery for oral cancer patients, it is essential to consider the use of elective tracheostomy based on preoperative assessment of the risk of postoperative airway obstruction using tools like the Cameron scoring system, and patients’ condition. Research confirms that elective tracheostomy effectively enhances airway management in patients with severe oral cancer.
Acetate-containing bicarbonate dialysate increases all-cause mortality more than acetate-free bicarbonate dialysate containing citrate in hemodialysis patients
Authors: Okada Kazuyoshi , Tashiro Manabu , Michiwaki Hiroyuki , Tanaka Yusaku , Yamashita Syo , Inoue Tomoko , Shima Hisato , Minakuchi Jun 
Published: July 2024

Abstract
Background
No studies have compared mortality between acetate-containing bicarbonate dialysate (ABD) and acetate-free bicarbonate dialysate containing citrate (AFD) in hemodialysis (HD) or online hemodiafiltration (OHDF). We therefore compared mortality between ABD and AFD in each modality.

Methods
This retrospective observational study included 738 patients who were receiving super high-flux (SHF) or SHF albumin-leaking HD (n = 310: ABD 235 and AFD 75) or OHDF (n = 428: ABD 321 and AFD 107) at our institution between 1 April and 1 July 2017. Three-year all-cause mortality was compared between ABD and AFD in the HD or OHDF groups using a propensity score matching model. Kaplan–Meier survival curves were compared using the log-rank test, and then Cox regression analysis with adjustments was performed for some covariates that remained significant.

Results
After propensity score matching, mortality on ABD was not significantly different from that on AFD in the HD group [n = 75; hazard ratio (HR) 2.271, 95% confidence interval (CI) 0.863–5.981, P = 0.087] or in the OHDF group (n = 107; HR 1.944, 95% CI 0.585–6.458, P = 0.269) without adjustments. However, with adjustments using some covariates, mortality was significantly higher on ABD than on AFD (adjusted HR 4.501, 95% CI 1.434–14.125, P = 0.010) in the HD group, but not in the OHDF group.

Conclusions
These findings suggest that ABD worsens mortality more than AFD in patients on SHF and SHF albumin-leaking HD.
Trial registration: UMIN Clinical Trials Registry, UMIN000053090. Prospectively registered 13 December 2023, https://center6.umin.ac.jp/cgi-bin/ctr/ctr_view_reg.cgi?recptno=R000060581.
Development of a flexible liver phantom for hepatocellular carcinoma treatment planning: a useful tool for training & education
Authors: Al-Thani Abdulla , Sharif Abdulrahman , El Borgi Sami , Abdulla Shameel , Ahmed Saleh Mahmoud Raja , Al-Khal Reem , Velasquez Carlos , Aboumarzouk Omar , Dakua Sarada Prasad 
Published: July 2024

Abstract
Purpose
Hepatocellular carcinoma (HCC) is one of the most common types of liver cancer that could potentially be surrounded by healthy arteries or veins that a surgeon would have to avoid during treatment. A realistic 3D liver model is an unmet need for HCC preoperative planning.

Methods
This paper presents a method to create a soft phantom model of the human liver with the help of a 3D-printed mold, silicone, ballistic gel, and a blender.

Results
For silicone, the elastic modulus of seven different ratios of base silicone and silicone hardener are tested; while for ballistic gel, a model using 20% gelatin and 10% gelatin is created for the tumor and the rest of the liver, respectively. It is found that the silicone modulus of elasticity matches with the real liver modulus of elasticity. It is also found that the 10% gelatin part of the ballistic gel model is an excellent emulation of a healthy human liver.

Conclusion
The 3D flexible liver phantom made from a 10% gelatin-to-water mixture demonstrates decent fidelity to real liver tissue in terms of texture and elasticity. It holds significant potential for improving medical training, preoperative planning, and surgical research. We believe that continued development and validation of such models could further enhance their utility and impact in the field of hepatobiliary treatment planning and education.
mpMRI features of mucinous prostate cancer: two case reports
Authors: Chiacchio Giuseppe , Grimaldi Alessandro , Beatrici Edoardo , Rossi Riccardo , Cappuccelli Simone , Pitoni Lucia , Lacetera Vito , Gabrielloni Giuliana , Rebonato Alberto , Scarcella Simone , Beatrici Valerio 
Published: July 2024

Abstract
Background
Mucinous adenocarcinoma of the prostate (MACP) is one of the rarest variants of prostatic neoplasm, with more aggressive behavior than non-mucinous prostatic cancer. Previous studies suggested that these tumors exhibit different imaging magnetic resonance imaging (MRI) features compared with those of non-mucinous adenocarcinoma of the prostate upon which the conventional PIRADS v2.1 is based. To the best of our knowledge, this case series is the first to describe the multiparametric magnetic resonance imaging features of the MACP.

Results
We presented two cases of biopsy proven mucinous adenocarcinoma of the prostate studied with multiparametric MRI. In both cases, diagnosis was late because of the different MRI features of MACP than those of the more common adenocarcinoma of the prostate. In both mpMRI, MACP appears to be hyperintense on T2WI, there was not a significant decrease in diffusivity in ADC maps and it exhibits early enhancement in DCE-MRI; the septa resulted in hypointense on T2WI compared to the PZ

Conclusions
According to our experience, the conventional PIRADS v2.1 score is not suitable for mucinous prostate adenocarcinoma. MACP appears to be hyperintense on T2WI, has a lower ADC value, and exhibits early enhancement in DCE-MRI; the septa are usually hypointense on T2WI compared to the PZ. It is imperative for radiologists and urologists to be cognizant of this rare variant of prostate cancer to promptly identify and diagnose it, thereby preventing any diagnostic delays.
The influence of IT outsourcing on organisational success and innovation
Authors: Murphy Liam 
Published: July 2024

AbstractThis study investigates the domain of IT outsourcing (ITO), focusing on its implications for organisational success or failure, and its impact on innovation. Through a comprehensive literature review, significant gaps were identified, including a lack of empirical studies assessing ITO outcomes for organisations, how ITO impacts innovation, and if an organisation’s industry impacts ITO success. In response, three research questions are formulated to address the identified gaps, leading to the creation of a novel conceptual model designed to evaluate the influence of ITO on organisations. Employing a deductive approach, the model informed a triangulation research approach, combining quantitative and qualitative data. A survey garnered responses from 112 senior IT professionals across three industries, complemented by eight interviews with senior and executive management. Findings indicate a concerning rate of perceived ITO failures and reveal that the specific industry of an organisation can affect ITO success. The study also concludes that ITO adversely impacts organisational innovation. Based on these insights, eight best practices are recommended to enhance ITO outcomes, including market research on vendor skillsets and experience, the establishment of well-structured contracts, adherence to SLAs without grace periods, shorter project durations, multi-sourcing strategies, bolstering internal IT capabilities, helping employees to understand ITO value, and rigorous governance. Acknowledging its limitations, this paper calls for future research involving broader industry representation and larger sample sizes to validate and expand upon these findings.
UCHL5 is a putative prognostic marker in renal cell carcinoma: a study of UCHL family
Authors: Zhang Mengdi , Li Jingxian , Liu Sijia , Zhou Fangfang , Zhang Long 
Published: July 2024

AbstractA macroscopic perspective is indispensable for understanding the intricate relationship between deubiquitinases and tumorigenesis. Proteomics has been proposed as a viable approach for elucidating the complex role of deubiquitylation in cellular progression. Instead of studying the function of a single ubiquitinase, research on a deubiquitinase family with similar catalytic core(s) may provide a new perspective for the pathological understanding of cancer. The Ubiquitin C-terminal hydrolase L (UCHL) family consists of four members: UCHL1, UCHL3, UCHL5, and BRAC1 associated protein-1 (BAP1), and they have been implicated in tumorigenesis and metastasis. Some members are considered hallmarks of intracranial lesions, colon cancer, chromatin remodeling, and histone stability. The present study uncovered an unknown correlation between the UCHL family and renal cancer. We discovered that UCHLs exhibit diverse regulatory effects in renal cancer, establishing connections between the renal cancer and truncated gene mutations, mitochondrial energetic metastasis, immune cell infiltration, and chromosomal stability of UCHLs family. Notably, we found that the increase of UCHL5 expression in renal cancer cells decreases the antigen processing and presentation of RCC tumor-infiltrating B cells. Further research identified that the expression of UCHL5 in RCC tumors is correlated with transport proteins, which led us to find that the abundance of UCHL5 in the blood of late-stage renal cell cancer patients is upregulated from 18 ng/L to 500 ng/L. Therefore, we propose that the abundance of UCHL5 in patients' blood can be a possible indicator of poor prognosis for renal cell cancer.
Efficacy of digital interventions on physical activity promotion in individuals with noncommunicable diseases: an overview of systematic reviews
Authors: Kardan Mohammadhossein , Jung Andres , Iqbal Mehvish , Keshtkar Saeed , Geidl Wolfgang , Pfeifer Klaus 
Published: July 2024

Abstract
Background
Physical inactivity is considered one of the main modifiable risk factors for noncommunicable diseases. It has been proven that an active lifestyle is an efficient means of preventing and managing noncommunicable diseases. Multiple barriers have been identified that hinder engagement in conventional physical activity programs and the achievement of physical activity recommendations. Digital interventions may expand opportunities to resolve these barriers and empower people with noncommunicable diseases to be physically active. This overview of systematic reviews aims to evaluate the efficacy of digital interventions on physical activity promotion among individuals with major noncommunicable diseases.

Method
A protocol was registered in the PROSPERO database (CRD42022364350). A qualitative synthesis method was applied to summarize the efficacy data. The assessment of methodological quality using AMSTAR-2 for each systematic review was performed by two independent reviewers.

Results
Searches in nine databases resulted in seven systematic reviews for inclusion. Most of the primary studies included in these seven reviews were conducted in high-income countries. The reviews addressed five populations: participants with cancers, cardiovascular diseases, chronic obstructive pulmonary disease, type 2 diabetes, and osteoarthritis. Digital interventions were classified into two categories: interventions based on digital activity trackers and remote interventions via digital facilities. Additional components were identified in both categories of interventions such as motivational content, counseling, goal setting, tailored feedback/reminders, and other behavioral change techniques. The overall quality of the reviews ranged from critically low to low. The findings provided some evidence that both categories of interventions were effective in promoting physical activity.

Conclusion
The findings of this overview suggest that some types of digital interventions, such as self-monitoring with digital activity trackers and remote interventions via digital facilities, may be effective means of promoting physical activity across major noncommunicable diseases. The findings should be interpreted cautiously since the methodological quality of the included reviews ranged from critically low to low, and there was no consistent assessment of the certainty of evidence. Further systematic reviews are required on efficacy of digital interventions with more rigorous conducting and reporting of systematic review methodologies.
Intratumoral Escherichia Is Associated With Improved Survival to Single-Agent Immune Checkpoint Inhibition in Patients With Advanced Non–Small-Cell Lung Cancer
Authors: Elkrief Arielle , Montesion Meagan , Sivakumar Smruthy , Hale Caryn , Bowman Anita S. , Begüm Bektaş Ayyüce , Bradic Martina , Kang Wenfei , Chan Eric , Gogia Pooja , Manova-Todorova Katia , Mata Douglas A. , Egger Jacklynn V. , Rizvi Hira , Socci Nicolas D. , Kelly Daniel W. , Rosiek Eric , Meng Fanli , Tam Grittney , Fan Ning , Drilon Alexander , Yu Helena A. , Riely Gregory J. , Rekhtman Natasha , Quintanal Villalonga Álvaro , Dogan Snjezana , Bhanot Umesh , Gönen Mithat , Loomis Brian , Hellmann Matthew D. , Schoenfeld Adam J. , Ladanyi Marc , Rudin Charles M. , Vanderbilt Chad M. 
Published: July 2024

PURPOSE
The impact of the intratumoral microbiome on immune checkpoint inhibitor (ICI) efficacy in patients with non–small-cell lung cancer (NSCLC) is unknown. Preclinically, intratumoral Escherichia is associated with a proinflammatory tumor microenvironment and decreased metastases. We sought to determine whether intratumoral Escherichia is associated with outcome to ICI in patients with NSCLC.

PATIENTS AND METHODS
We examined the intratumoral microbiome in 958 patients with advanced NSCLC treated with ICI by querying unmapped next-generation sequencing reads against a bacterial genome database. Putative environmental contaminants were filtered using no-template controls (n = 2,378). The impact of intratumoral Escherichia detection on overall survival (OS) was assessed using univariable and multivariable analyses. The findings were further validated in an external independent cohort of 772 patients. Escherichia fluorescence in situ hybridization (FISH) and transcriptomic profiling were performed.

RESULTS

In the discovery cohort, read mapping to intratumoral Escherichia was associated with significantly longer OS (16
v
11 months; hazard ratio, 0.73 [95% CI, 0.59 to 0.92];
P
= .0065) in patients treated with single-agent ICI, but not combination chemoimmunotherapy. The association with OS in the single-agent ICI cohort remained statistically significant in multivariable analysis adjusting for prognostic features including PD-L1 expression (
P
= .023). Analysis of an external validation cohort confirmed the association with improved OS in univariable and multivariable analyses of patients treated with single-agent ICI, and not in patients treated with chemoimmunotherapy. Escherichia localization within tumor cells was supported by coregistration of FISH staining and serial hematoxylin and eosin sections. Transcriptomic analysis correlated Escherichia-positive samples with expression signatures of immune cell infiltration.

CONCLUSION
Read mapping to potential intratumoral Escherichia was associated with survival to single-agent ICI in two independent cohorts of patients with NSCLC.