bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combining Interferon-γ Release Assays and Metagenomic Next-generation Sequencing for Diagnosis of Pulmonary Tuberculosis: A Retrospective Study
Authors: Liu Yanyan , Fang Miaohong , Yuan Chenxi , Yang Yi , Yu Liang , Li Yasheng , Hu Lifen , Li Jiabin 
Published: July 2024

Abstract

Background
Rapid diagnosis of pulmonary tuberculosis (PTB) is urgently needed. We aimed to improve diagnosis rates by combining tuberculosis-interferon (IFN)-γ release assays (TB-IGRA) with metagenomic next-generation sequencing (mNGS) for PTB diagnosis.
Methods
A retrospective study of 29 PTB and 32 non-TB patients from our hospital was conducted between October 2022 and June 2023. Samples were processed for TB-IGRA and mNGS tests according to the manufacturer’s protocol.
Results
The levels of IFN-γ release in PTB patients were significantly higher than those -in non-TB patients (604.15 ± 112.18 pg/mL, and 1.04 ± 0.38 pg/mL, respectively; p < 0.0001). Regarding presenting symptoms or signs, cough and thoracalgia were less common in PTB patients than in non-TB patients (p = 0.001 and p = 0.024, respectively). Total protein and albumin levels in the sera of PTB patients were significantly elevated compared to non-TB patients (p = 0.039 and p = 0.004, respectively). The area under the ROC curve (AUC) for TB-IGRA in PTB diagnosis was 0.939. With an optimal IFN-γ cut-off value of 14.3 pg/mL( Youden’s index 0.831) sensitivity was 86.2% and specificity was 96.9%. ROC curve analysis for mNGS and TB-IGRA combined with mNGS showed AUCs of 0.879 and 1, respectively.
Conclusions
TB-IGRA combined with mNGS is an effective method for diagnosing tuberculosis, and can be used in the clinical diagnosis of PTB.
Implausible, Not Impossible: Delayed Supradiaphragmatic Thoracic Migration of a Ventriculoperitoneal Shunt in a 17-Month-Old
Authors: FLORIAN Ioan-Alexandru , TOPAL Paula , FLORIAN Teodora-Larisa , FONT Dragos , FLORIAN Ioan-Stefan 
Published: July 2024

Abstract

Background
Intrathoracic migration of a ventriculoperitoneal shunt (VPS) is a phenomenally rare complication, with the supradiaphragmatic intercostal variant even more so. Whereas it can prove debilitating or even fatal via massive hydrothorax, the causative mechanism and proper management of this occurrence are undefined.
Case Presentation:
A 17-month-old girl who had undergone VPS insertion at one month of age was brought to our department for somnolence and dyspnea, which had a sudden onset. Despite a previous thoracostomy provided temporary symptom relief, she had relapsed. Computed tomography (CT) of the chest showed a large loop of the right-sided VPS penetrating into the thorax through the anterior wall, as well as marked right hydrothorax. She was subjected to VPS revision and thoracostomy, with the swift, complete, and lasting remission of her complaints.
Conclusion
It is possible that local reaction coupled with negative inspiratory pressure caused the catheter loop to break into the pleural cavity. Our case demonstrates an exceedingly rare event that has a favorable prognosis if diagnosed and treated quickly and appropriately.
Discrete wavelet analysis of acoustic emission precursory characteristics of confining pressure failure in Coal and rock triaxial unloading
Authors: Ma Shaojie , Zhou Yan , Ma Depeng 
Published: July 2024

Abstract

Acoustic emission information can describe the damage degree of rock samples in the process of failure. However, as a discrete non-stationary signal, acoustic emission information is difficult to be effectively processed by conventional methods, while wavelet analysis is an effective method for non-stationary signal processing. Therefore, acoustic emission signal is deeply studied by using wavelet analysis method. In this paper, on the basis of noise reduction of acoustic emission signal, Matlab calculation program is used to decompose the acoustic emission signal of coal sample under the confining pressure test of triaxial unloading, and the singularity detection is carried out. The results show that the time when the Lipschitz index value first appears α negative can be used as the prediction time. However, the corresponding time when the Lipschitz index value is -0.15~-0.31 should be excluded; The absolute range of the difference between the final forecast time and the actual rupture time of coal samples is [5.2s, 17.1s], and the coal samples with the absolute value of time difference within [5.2s, 10.0s] account for 63.6% of the total.
Hemolysis intraoperative monitoring by Helge V-test in patients undergoing extracorporeal circulation in cardiac surgery: a single center data
Authors: Schiavoni Lorenzo , Stifano Mariapia , Mattei Alessia , Verde Francesca La , Strumia Alessandro , Pumpo Annalaura Di , Sarubbi Domenico , Rizzo Stefano , Conti Maria Cristina , Mortini Lara , Cataldo Rita , Costa Fabio , Pascarella Giuseppe , Rita Alessandro , Casali Elena , Agrò Felice Eugenio , Carassiti Massimiliano 
Published: July 2024

Abstract

Purpose
Hemolysis is a complication in all surgical procedures requiring extracorporeal circulation (ECC). The aim of this study is to evaluate the effectiveness of the disposable point of care device Hemcheck Helge™ V-TEST quantifying hemolysis during cardiac surgery in ECC.
Methods
Two whole blood samples of 78 patients were simultaneously collected at different times of surgery: T0 pre ECC; T1 at the time of clamping of aorta; T2 at 20 minutes after the start of ECC; T3 at the end of ECC; T4 at the end of surgery. Both samples were analyzed by the disposable Hemcheck Helge™ V-TEST device, which offers a real-time assessment of hemolysis through a value of plasma free hemoglobin (PfHb) expressed in mg/dL.
Results
Out of all cases analyzed, no case of hemolysis (PfHb ≥ 50 mg/dL) was recorded at T0. The results recorded median PfHb values at T0 0.5 (0-7.1) mg/dL, at T1 3.75 (0-14.4) mg/dL; at T2 8.25 (0.4–19.1) mg/dL, at T3 27.5 (9.9–50) mg/dL, at T4 18.5 (2.4–41) mg/dL; for all T time p value was < 0.001. A statistically significant correlation was recorded between hemolysis values > 50 mg / dL at T3 and ECC time > 100 minutes (p < 0,05).
Conclusions
The use of Hemcheck Helge™ V-TEST allows effective identification of hemolysis directly in the operating room, reducing wasted time for laboratory analyses. This could help the anesthetist, perfusionist or cardiac surgeon approaching earlier intraoperative hemolysis and its effects on organs function and improve the post-operative course of patients undergoing cardiac surgery in ECC.
Providing excellence of care through the development and implementation of Digital Care Pathway across Northern Health, Australia: A co-designed methodological protocol
Authors: Duckham Rachel L , Webster Tracey , Gan Min , Gust Anthony , See Katharine 
Published: July 2024

Abstract

Background: Healthcare costs within Australia continue to rise without commensurate improvement in health outcomes of care delivered. Across Australia there is a commitment to reform the healthcare system focusing on value-based care for patients. Within Victoria Australia, Northern Health have sought to enhance communication with patients and their families, particularly around shared decision making, quality of life and symptoms, and the impact of the patient care and treatment. This paper describes the co-design methodology utilised to: 1) Develop and implement new digital models of care, 2) capture, utilise and analysis patient reported outcome measures, and 3) evaluate the effectiveness and cost effectiveness of the transformed models of care across the health service.
Methods: A six-phase co-design methodology incorporating the Kotters change model will be piloted to develop, refine, implement and evaluate innovative multi-lingual, fully automated digital care pathways across ten clinical services. Quantitative and qualitative data will be collected from consumers and clinicians during the development and implementation of the digital care pathway to 1) understand the barriers and enablers of clinical care across specific clinical departments, 2) provide information to inform decision making, 3) understand patient outcomes and inform treatment, and 4) to evaluate each implemented digital care pathway.
Discussion: Northern Health is leading the change in Australia to implement evaluate and redesign models of care based on evidence to maximise the impact on health outcomes for patients and the community. This innovative initiative will change the way in which healthcare is delivered. At the time of publication this methodological approach has been applied with one digital care pathway implementation and 9 are currently under development.
Fusing fact and mask mechanism to promote consistency between long text summarization and fact
Authors: Wang Xianchuan , Lu Ben , Ming Wenkai , Wang Xianchao 
Published: July 2024

Abstract

The basic requirement of text summarization is that the facts in the summary and the original text must be consistent. At present, most of the summarization models choose to introduce fact information in the decoding stage. With the increase of the text content, the ability to process fact information becomes weak, which leads to fact consistency errors in the model. From the perspective of data fusion in input section, this paper proposes a LTSum-FTL (Long Text Summarization model with Fact Triples Labeling) to improve factual consistency, which help readers obtain more accurate information. Firstly, use fact triples to represent the factual information of the original text. Then annotate the three attributes in the triple, and vector the annotation information and fuse into the input vector. Finally, use the improved masking mechanism to mask or replace the triple attributes of the input part, to improve the model summary ability. The experimental results show that the proposed model can effectively reduce the probability of fact consistency errors,it is at least 2.4%,1.1% and 0.3 higher than that of other comparison models in the metrics of Pre1,Pre2 and FactCC-Socre.
The comparison of the risk of neurological deterioration and clinical outcomes in different atherosclerotic stroke patterns
Authors: He Yue , Zhang Tingting , Wu Wenqi , Niu Kai , Cai Zhirong , Zhao Tian , Shao YuanWei , Yang Yi , Zhu Ying , Yu Ming 
Published: July 2024

Abstract

This study aims to compare the incidences of ND and poor outcome (a modified Ranking scale > 2 points at discharge) among patients with different atherosclerotic stroke patterns. A total of 688 participants were categorized into 4 groups according to atherosclerotic stroke pattern: multiple small infarcts (MSI), single subcortical infarction (SSI), borderzone infarct (BZI) and large infarct groups. Among the 4 groups, MSI group had the lowest incidences of ND and poor outcome (13.5% and 16.2%, respectively). In multivariable analyses, for BZI patients, the risks of ND [odds ratio (OR) = 4.33, 95% confidence interval (CI) = 2.37–7.94, p< 0.001] and poor outcome (OR = 4.16, 95% CI = 2.04–8.50, p < 0.001) both increased approximately 3-fold than MSI, both of which were the highest among the 4 stroke patterns. The neutrophil to lymphocyte ratio in BZI and large infarct groups were higher than in MSI and SSI groups [3.35 (2.28, 5.04) and 3.36 (2.53, 4.94) vs. 2.64 (1.89, 4.06) and 2.71 (1.93, 3.91), p< 0.001]. BZI group had the highest risks of ND and poor outcome among atherosclerotic stroke patients. BZI and large infarct patients had stronger poststroke inflammation than MSI and SSI patients.
Insulin resistance, left atrium enlargement, and recurrence in patients with atrial fibrillation undergoing radiofrequency ablation
Authors: Luo Yan , Qin Shujuan , Huang Wenchao , Yang Guoshu , Luo Duan , Sun Huaxin , Tang Yan , Xu Bao , He Guijun , Yang Yongxin , Tong Lin , Cai Lin , Zhang Zhen , Liu Hanxiong , Xiong Shiqiang 
Published: July 2024

Abstract

Aims: To investigate the association between insulin resistance (IR), left atrial enlargement, and atrial fibrillation (AF) recurrence in AF patients undergoing radiofrequency catheter ablation (RFCA).
Methods: 910 AF patients were evaluated for IR using TyG-BMI and left atrial enlargement using LAD. Various analytical methods were employed to study the relationship between TyG-BMI, LAD, and AF recurrence.
Results: Over a median 12-month follow-up period, 189 (20.77%) cases of AF recurrence were observed. The likelihood of AF recurrence rose with elevated TyG-BMI and LAD levels. After adjusting for multiple factors, both TyG-BMI and LAD were significant predictors of AF recurrence. Both TyG-BMI and LAD demonstrated a positive dose-response relationship with AF recurrence. The areas under the ROC curves for TyG-BMI and LAD in predicting AF recurrence were 0.634 and 0.639, respectively. Notably, LAD partly mediated the association between TyG-BMI and AF recurrence, accounting for an 19.23% proportion.
Conclusion: Elevated baseline TyG-BMI and LAD were associated with a higher risk of AF recurrence in patients undergoing RFCA. Furthermore, the enlargement of the left atrium partially mediated the relationship between IR and AF recurrence. Ameliorating IR may serve as a potential upstream therapy for the comprehensive management of AF in clinical practice.
Characterization and Structural Basis for the Brightness of mCLIFY: A Novel Monomeric and Circularly Permuted Bright Yellow Fluorescent Protein
Authors: Goldman Yale , Shweta Him , Gupta Kushol , Zhou Yufeng , Cui Xiaonan , Li Selene , Lu Zhe , Dantzig Jody 
Published: July 2024

Abstract

We present mCLIFY: a monomeric, bright, yellow, and long-lived fluorescent protein (FP) created by circular permutation of YPet, the brightest yellow FP from Aequorea Victoria for use in cellular and in vitro single molecule studies. mCLIFY retains the enhanced photophysical properties of YPET as a monomer at concentrations ≤ 40 μM. In contrast, we determined that YPet has a dimerization dissociation constant (KD1-2) of 3.4 μM. Dimerization of YPet can cause homo-FRET, which underlies quantitative errors due to dimerization and homo-FRET. We determined the atomic structure of mCLIFY at 1.57 Å resolution and used its similarity with Venus for guided chromophore-targeted substitution studies to provide insights into its enhanced photophysical properties. The mutation V58L within the chromophore pocket improved quantum yield and extinction coefficient, making mCLIFY ~30% brighter than Venus. The extensive characterization of the photophysical and structural properties of YPet and mCLIFY presented here allowed us to reveal the basis of their long lifetimes and enhanced brightness and the basis of YPet’s dimerization.
Comprehensive genomic profiling of Taiwanese triple negative breast cancers with medium- and large-sized sequencing panels: a comparative study of actionable genes
Authors: Huang Chi-Cheng , Yeh Yi-Chen , Tsai Yi-Fang , Lin Yen-Shu , Chao Ta-Chung , Liu Chun-Yu , Ho Hsiang-Ling , Tseng Ling-Ming 
Published: July 2024

Abstract

Introduction:
Comprehensive genomic profiling (CGP) is a molecular diagnostic tool with increasing use in cancer research and treatment. There are several commercialized CGP assays with variable targeted genes, however, how large a panel should be used for breast cancer remains undetermined.
Methods
Triple negative breast cancer (TNBC) patients from the VGH-TAYLOR study were initially assayed by a medium-sized CGP panel (Oncomine Comprehensive Panel, OCP, v3), and the remaining nucleic acid specimens were re-sequenced with a large-sized CGP panel (TruSight Oncology 500, TSO500). Molecular profiling between the two sequencing panels was compared and reported.
Results
A total of 108 breast cancers were successfully assayed using both platforms and 272 variants were reported at least once by OCP or TSO500. Variants reported were among actionable genes (AKT1, BRCA1/2, PALB2, ERBB2, PIK3CA, PTEN) and TP53. Concordance rate between TSO500 and OCP was 34.6% and was enhanced to 58.9% after excluding polymorphisms, out-of-targeted region variants and those with low variant allele frequency (< 10%).
Conclusion
Only one-third of actionable mutations could be detected consistently between the medium- and the large-sized CGP panels using the default analytical pipelines, while extensive bioinformatics analyses improved variant calling consistency substantially. TSO500, the larger panel, detected more variants than OCP from the same set of actionable genes.
Preoperative anxiety and its association with resilience of surgical patients in the preoperative waiting area: A latent profile analysis
Authors: Shen Xiaowen , Wu Min , Wang Rong , Yang Silan , Wang Yuwei , Dai Suwan , Zhou Qinghe 
Published: July 2024

Abstract

Background
Preoperative anxiety is a specific type of anxiety that focuses on concerns and worries related to anesthesia and surgical procedures. However, there has been limited research conducted on the various aspects of this phenomenon, especially in waiting areas.
Objective
Using latent profile analysis (LPA), this study aimed to identify various preoperative anxiety profiles among surgical patients in the preoperative waiting area. Additionally, this study aimed to explore the demographic and resilience factors associated with different preoperative anxiety profiles.
Methods and Materials
A cross-sectional study was conducted at comprehensive hospitals in Jiaxing, Zhejiang Province, China. Three hundred seventy-three (373) surgical patients were included in the analyses. Surgical patients completed the demographic characteristics questionnaire, the Amsterdam Preoperative Anxiety and Information Scale (APAIS-C), and the Connor-Davidson Resilience Scale (CD-RISC) while waiting for surgery in the waiting area.
Results
The LPA results indicated that the data were most consistent for four types of solutions: high preoperative anxiety-medium information need, high preoperative anxiety-low information need, low preoperative anxiety-medium information need, and low preoperative anxiety-low information need. One-way ANOVA revealed significant differences between the four groups with respect to resilience. Sex, education background, tumor diagnosis, sleep quality and surgical experience significantly predicted profile membership.
Conclusion
There is heterogeneity in preoperative anxiety among surgical patients in the waiting area. When supporting surgical patients in waiting areas, nurses should consider the level of psychological resilience of patients in addition to assessing demographic factors to identify high-risk subgroups to warrant intervention.
Investigation of Cell-to-cell Transfer of Polystyrene Microplastics Through Extracellular Vesicle-mediated Communication
Authors: Kim Nara , Park Joo Hyun , Lee Inha , Jung Gee Soo , Lee Jae Hoon , Lee Min Jung , Im Wooseok , Cho SiHyun , Choi Young Sik 
Published: July 2024

Abstract

Plastics have become an integral part of human life, and their production is increasing annually. Plastics are broken down into small particles known as microplastics (MPs) with particle size of < 5 mm in the environment because of a variety of factors. MPs are prevalent in the environment and all living organisms are exposed to their effects. In this study, we investigated whether polystyrene (PS)-MPs were transferred from cell-to-cell via extracellular vesicles (EVs). This study showed that cell-derived EVs could transport plastic particles. In addition, using a real-time imaging device, we confirmed that PS-MPs were transported by EVs that accumulated in the cells. This study provides an understanding of the potential effects of PS-MPs on living organisms via EVs and suggests directions for future research.
Hemolytic disease of fetus and newborn course, management and outcomes - an analysis based on the Israeli Maccabi database
Authors: Fallach Noga , Hoshen Moshe , Gazit Sivan , Segal Yaakov , Fitzgibbon Marie , Karmous Wisam , Barthelmes Norma , Iqbal Sofia , Borsi Andras , Noel Wim , Mandel Dror , Yinon Yoav 
Published: July 2024

Abstract

Objective: Hemolytic disease of the fetus and newborn (HDFN) can cause severe prenatal and postnatal outcomes. The main objective of this study is to understand the clinical impact of HDFN on pregnant women and newborns.
Study Design: A retrospective cohort study was performed on de-identified data extracted from a large nationwide health organization. The cohort included women and newborns diagnosed with HDFN between 1998-2021. Cohort characteristics and outcomes are described.
Result: Over the 24-year study period, incidence rate of HDFN among pregnant women was stable while incidence rate among newborns declined. Severe HDFN was diagnosed in 28.8% of 73 HDFN affected pregnancies. One third of 450 HDFN newborns were diagnosed with anemia or jaundice; 5 cases of kernicterus were observed.
Conclusion: Severe prenatal and postnatal outcomes following HDFN were observed. Further studies are needed to evaluate treatment regimens and assess the association between treatment management and short- and long-term outcomes.
Effect of long-term fertilization on the growth and yield formation of early rice
Authors: Hu Zhihua , Liu Lailou , Xu Xiaolin , Hu Dandan , Song Huijie , Wu Yan , Wu Jianfu 
Published: July 2024

Abstract

Fertilization is crucial for rice growth and yield formation. We conducted a 42-year long-term fixed experiment in southeast China, examining nine treatments. This study focused on three treatments: a combination of chemical N, P, and K (NPK), a double dose of chemical N, P, and K (HNPK), and a combination of chemical fertilizer and organic fertilizers (NPKM). We assessed rice yield, yield components, tiller dynamics, dry matter accumulation, chlorophyll dynamics, and leaf transcriptome at the full heading stage. Results indicated that early rice yield followed the order of NPKM > HNPK > NPK. Compared to NPK, HNPK and NPKM significantly increased spikelet density, effective panicles, and 1000-grain weight, while also promoting tillering. NPKM and HNPK significantly enhanced dry matter accumulation from the full heading stage to the filling stage and facilitated the transport of dry matter from leaves and stems to spikes during the filling to mature stages. NPKM consistently maintained higher chlorophyll content than HNPK and NPK at all stages, significantly reducing chlorophyll decline from the full heading stage to the filling stage. Correlation analysis revealed a significant positive relationship between yield and both chlorophyll content and dry matter accumulation under long-term fertilization. There was also a significant negative correlation between yield and chlorophyll reduction from the full heading stage to the filling stage. Differential gene expression analysis at the full heading stage showed significant enrichment in photosynthesis and plant senescence metabolism pathways among different fertilization treatments. Overall, the combined application of chemical and organic fertilizers significantly increased early rice yield by enhancing tillering, regulating photosynthesis and senescence-related gene expression, boosting dry matter accumulation from the full heading stage to the filling stage, and improving dry matter transport to spikes from the filling to the mature stage.