bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between gut microbiota and breast cancer: a Mendelian randomization analysis of mediating effects based on inflammatory proteins
Authors: Li Haipeng , Li Hui , Liu Yuan , Chen Chen , Liu Xinyu , Li Hongtao 
Published: August 2024

Abstract

Background: Breast cancer is associated with dysbiosis of dysbiosis of gut flora. However, the mechanisms of how gut microbiota mediate breast cancer disease are not clear, and exploring possible mediating mechanisms is key to investigating the study of the mechanisms that gut microbiota contribute to breast cancer development.
Methods: A two-sample two-way Mendelian randomization (MR) analysis was employed, with publicly available genome-wide association study (GWAS) data, to investigate role of abnormal gut microbiota in breast cancer development. It focuses on assessing the potential mediation by circulating inflammatory proteins. The primary methodology employed for identifying gut microbiota (GM) associated with breast cancer and examining the impact of inflammatory markers is the variance inverse weighting method, supplemented by the MR-Egger method and weighted median method. This research aims to offer novel insights into potential therapeutic interventions for breast cancer.
Result: Seven genera and three inflammatory proteins were determined to be associated with breast cancer. The inflammatory protein Fibroblast growth factor 21 levels mediated the effect of the intestinal bacterium Adlercreutzia on the course of breast cancer in 11.1% of cases, and the inflammatory protein CD40L receptor levels mediated the effect on the gut microbiotaParabacteroides on the course of breast cancer in 9% of cases.
Gene Expression Profiling for the Diagnosis of Male Breast Cancer
Authors: Liu Jing , Sun Yifeng , Qi Peng , Wo Yixin , Xu Qinghua , Huang Shenglin , Wang Qifeng 
Published: August 2024

Abstract

Background:
Male breast cancer (MBC) is a rare malignancy, but its global incidence has shown a notable increase in recent decades. Factors such as limited health literacy, insufficient health education, and reluctance to seek medical attention contribute to most MBC patients being diagnosed at an advanced stage. Consequently, there is an urgent need for a highly specific and sensitive diagnostic approach to MBC.
Methods:
This retrospective study enrolled 20 patients with 30 surgical or biopsy MBC specimens from August 2020 to August 2023. The 90-gene expression assay was performed to determine the tissue of origin. Predicted tumor types were then compared to the reference diagnosis for accuracy calculation. The differentially expressed genes were identified between male and female breast cancer.
Result:
The 90-gene expression assay demonstrated an overall accuracy of 96.7% (29/30) when compared with the pathological diagnosis. Subgroup analysis revealed accuracies of 100% (15/15) for primary tumors, 90.9% (10/11) for lymph node metastatic tumors, and 100% (4/4) for distant metastatic tumors. Five genes (RPS4Y1, PI15, AZGP1, PRRX1, and AGR2) were up-regulated, and six (XIST, PIGR, SFRP1, PLA2G2A, S100A2, andCHI3L1) were down-regulated in MBC.
Conclusion:
Our findings highlight the promising performance of the 90-gene expression assay in accurately identifying the tumor origin in MBC. Incorporating this assay into pathological diagnoses has the potential to empower oncologists with precision treatment options, ultimately enhancing the care and outcomes for patients with MBC.
Cardiac adverse events associated with statins in myocardial infarction patients: a pharmacovigilance analysis of the FDA Adverse Event Reporting System
Authors: Deng Chuanhuan , Lin Xiaofang , Ni Dan , Yuan Ludong , Li Jing , Liu Yuxuan , Liang Pengfei , Jiang Bimei 
Published: August 2024

Abstract

Background: Despite the advent of new pharmacotherapies, statins remain a cornerstone in the secondary prevention of myocardial infarction (MI). However, the cardiac adverse events (CAEs) linked to statins are not well-documented.
Aim: This pharmacovigilance study used data from the FDA Adverse Event Reporting System (FAERS) to investigate the association between statin use and CAEs in MI patients.
Method: Reports from the FAERS database (2004-2023) identifying statins as the primary suspect in MI patients were analyzed. The study evaluated seven types of statins: atorvastatin, fluvastatin, lovastatin, pitavastatin, pravastatin, rosuvastatin, and simvastatin. Disproportionality analysis using four major indices, Reporting Odds Ratio (ROR), Proportional Reporting Ratio (PRR), Bayesian Confidence Propagation Neural Network (BCPNN), and Multi-Item Gamma-Poisson Shrinker (MGPS), was conducted to detect signals of statin-related CAEs.
Results: Of the 20,346,289 reports reviewed, 150 identified statins as the primary suspect drug in MI patients. The most common CAEs were recurrent MI (50 reports), acute MI (14 reports), followed by tachycardia (10), angina pectoris (8), coronary artery occlusion (6), cardiac failure (6), and arrhythmia (6). The analysis revealed no significant signals of statin-induced CAEs.
Conclusion: The findings confirm that statin use in MI patients does not significantly increase the risk of cardiac adverse effects, supporting their safety profile in this context.
Thermodynamic processes-induced prolongating effects on triple La Niña events in a hybrid coupled ocean-atmosphere model
Authors: DONG Xiang , Zhang Rong-Hua , HU Junya , GAO Chuan , Chen Maonan 
Published: August 2024

Abstract

Observed ENSO cycles show apparent asymmetry, especially manifested as frequent occurrences of multi-year La Niña events but mostly single-year El Niño events, whose causes are still not well understood. Most previous research has primarily based on observational data and reanalysis products; current coupled ocean-atmosphere models still have difficulties in realistically depicting multi-year La Niña events. A new hybrid coupled model (HCM)-based 1000-year simulation presented here can adequately depict the evolution of triple La Niña events, with sensitivity experiments further performed to explore their physical mechanisms. The results reveal that off-equatorial Pacific Meridional Mode (PMM)-like sea surface temperature (SST) anomalies can promote triple La Niñas by establishing a surface cooling in the first place that is located in the central equatorial Pacific. When the PMM-related off-equatorial cold SST anomalies weaken, the equatorial surface cooling in the western-central equatorial Pacific can be sustained by thermodynamic coupling processes (wind speed–evaporation–SST feedback). Further sensitivity experiments and diagnostic analyses are conducted to confirm that the thermodynamic processes can prolong the triple La Niña events by intensifying the effect of the off-equatorial cold SST anomalies on the equatorial surface cooling and enhancing the local ocean-atmosphere coupling. Therefore, adequately representing the PMM-like SST anomalies and the related thermodynamic processes is an important factor affecting a way for coupled models to depict multi-year La Niña events.
Time to Positivity of Coagulase Negative Staphylococcus in Neonatal Blood Cultures as a Useful Tool to Discriminate Between Sepsis and Contamination
Authors: Carbonell-Sahuquillo Silvia , Olea Beatriz , Pérez-Suárez Raquel , Giménez Estela , Colomina Javier , Navarro David , Estañ Javier 
Published: August 2024

Abstract

Objective: to assess the usefulness of time to positivity (TTP) to distinguish between sepsis and contamination in coagulase-negative staphylococci (CoNS) isolates.
Study design: unicentric retrospective observational. 168 patients with suspected sepsis and positive blood culture for CoNS were recruited. Patients were subdivided into sepsis (29%) and probable contamination (71%). Logistic regression analyses were performed to evaluate different risk factors and clinical signs and symptoms associated with sepsis.
Results: TTP cut-off value that best discriminated sepsis from contamination was found to be 18 hours. Regression analysis revealed that TTP ≤18h, gestational age ≤32 weeks, taquicardia/bradicardia and hypoactivity/lethargy were independent predictors of sepsis.
Conclusion: usefulness of TTP to distinguish sepsis from contamination in isolation of CoNS, especially in neonates with other associated risk factors (lower gestational age or being a CVC carrier). The clinical signs that most increase the discriminatory power of TTP are the presence of tachycardia/bradycardia or hypoactivity.
Coronary Flow Velocity Reserve is Decreased in Patients with Restless Leg Syndrome; Case Control Study
Authors: Güz Göksel , Demirgan Serdar 
Published: August 2024

Abstract

Background
Restless leg syndrome (RLS) has been associated with an increased risk of vascular disorders, which suggests that endothelial dysfunction plays important role in the pathogenesis of RLS. In this study, we aimed to evaluate coronary endothelial dysfunction in RLS patients using coronary flow velocity reserve (CFVR) and compared it with healthy controls.
Methods
In this study, the participants were divided into two groups as group RLS (n=42) and group HC (n=41). The primary outcome was the CFVR compared between groups. The number of participants with a CFVR value below 2.0 was also evaluated. In addition, a correlation between the international restless legs scale (IRLS) and CFVR, white-blood-cell-count (WBC), C-reactive-protein (CRP) were analyzed. Secondary outcomes were the WBC, hemoglobin, CRP, blood glucose, high-density-lipoprotein (HDL), low-density-lipoprotein (LDL), and creatinine compared between the two groups.
Results
In the group RLS, CFVR was measured lower than healthy controls (p<0.001). When the groups were compared in terms of the number of participants with a CFVR less than 2.0, the difference between the groups was significant (p<0.001, 0/41 in group HC and 14/42 in group RLS).
Patients with RLS had higher WBC and CRP values. There was a negative correlation between CFVR and IRLS (p<0.001). The relationship between WBC, CRP, and IRLS was not statistically significant (p=0.691).
Conclusions
We conclude that RLS patients had lower CFVR compared with healthy controls and there was a negative correlation between the severity of RLS and CFVR. Therefore, we think that it may be beneficial to follow up patients with RLS in terms of coronary heart disease.
The reduced cognitive load of strong self-related information’s orientation attention revealed by ERP Running title: Cognitive Load of Self-Related Information
Authors: Cheng Sizhe , Zhan Qingchen , Qiu Rui , Xing Wanying , Zhang Yuting , Cao Yang , Li Xinhong , Huang Yan , Wang Xiuchao , Liu Xufeng 
Published: August 2024

Abstract

Background
Event-related potentials (ERPs) can demonstrate the dynamic nature of individual cognitive processing in real time through changes in brain neurons. Previous studies have shown that self-related information (SRI) has the advantage of orientation attention. P2, N2, and P300 are the main components related to the orientation attention of the SRI.
Methods
This study adopted the oddball paradigm of novel stimuli combined with time-domain and frequency-domain ERP analysis. 35 freshmen were recruited for this study.
Results
Compared with low SRI, high and medium SRI elicited similar temporal-occipital N170. High SRI produced N2/N250 components in the left temporal occipital region while medium SRI induced larger temporal-occipital P2. However, high and medium SRIs failed to elicit the P3a associated with orientation attention. There were significant differences in differing degrees of SRI in the delta and beta2 bands.
Conclusion
Birthplace, as a high SRI, can be automatically processed because it is not limited to word processing. Rather, it relies on emotional activation. High SRI needs less activation at a perceptual level, suggesting that it requires a smaller cognitive load.
Identification and validation of key extracellular proteins as the potential biomarkers in diabetic nephropathy
Authors: Pan Wei , Zhang Qiankun , Gong Xiaohua , Wu Wenjun , Zhou Qi 
Published: August 2024

Abstract

Objective: Accumulation of extracellular matrix (ECM) proteins in the glomerular mesangial region is a typical hallmark of diabetic nephropathy (DN). However, the molecular mechanism underlying ECM accumulation in the mesangium of DN patients remains unclear. The present study aims to establish a connection between extracellular proteins and DN with the goal of identifying potential biomarkers for this condition.
Methods: Differentially expressed genes (DEGs) between DN kidney tissue and healthy kidney tissue were analyzed using the public data GSE166239. Two gene lists encoding extracellular proteins were then utilized to identify extracellular protein-differentially expressed genes (EP-DEGs). Functional enrichment analyses, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, were performed on these EP-DEGs. A protein-protein interaction (PPI) network was established to identify key EP-DEGs. Furthermore, the diagnostic ability, immune cell infiltration, and clinical relevance of these EP-DEGs were investigated. Immunohistochemistry (IHC) staining of paraffin-embedded renal tissues was performed to validate the accuracy of the bioinformatic results.
Results: A total of 1204 DEGs were identified, from which 162 EP-DEGs were further characterized by overlapping with extracellular protein gene lists. From the PPI network analysis, five EP-DEGs (e.g., TNF, COL1A1, FN1, MMP9, and TGFB1) were identified as candidate biomarkers. TNF, COL1A1, and MMP9 had a high diagnostic accuracy for DN. Assessment of immune cell infiltration revealed that the expression of TNF was positively associated with resting dendritic cells (DCs) (r = 0.85, P < 0.001) and M1 macrophages (r = 0.62, P < 0.05), whereas negatively associated with regulatory T cells (r = -0.62, P < 0.05). Nephroseq v5 analysis demonstrated a negative correlation between the estimated glomerular filtration rate (eGFR) and TNF expression (r = -0.730, P = 0.025). Gene set enrichment analysis (GSEA) revealed significant enrichment of glycosaminoglycan (GAG) degradation in the high-TNF subgroup. IHC staining of renal tissues confirmed significantly elevated TNF-a expression and decreased hyaluronic acid (HA) levels in the DN group compared to controls (both P < 0.05), with a negative correlation observed between TNF-a and HA (r = -0.691, P = 0.026).
Conclusion: Our findings suggest that TNF plays a pivotal role in the pathogenesis of DN, potentially by activating the GAG degradation pathway. This study sheds light on the underlying mechanisms driving ECM accumulation in DN and identify TNF as a potential therapeutic target and biomarker for DN.
Risk Factors for Dehiscence in Alveolar Ridge Resorption with Patient-Specific Titanium Mesh Augmentation: A Retrospective Cohort Study
Authors: Kaya Sebahat , Barcellos Bharbara Marinho , Fan Shengchi , Azaripour Adriano , Walter Christian , Schiegnitz Eik , Sagheb keyvan 
Published: August 2024

Abstract

Purpose
This retrospective cohort study aimed to analyze the incidence of dehiscence after bone augmentation using patient-specific titanium meshes and the factors associated with its occurrence.
Material and Methods
Patients who underwent bone augmentation with the aid of patient-specific titanium mesh between December 2014 and October 2021 from the Department of Maxillofacial Surgery at Mainz University Hospital were included. The primary outcome was the occurrence of dehiscence after bone augmentation with a customized titanium mesh technique. The occurrence of dehiscence was recorded during the following time phases, enabling the determination of whether dehiscences occur early (< 2 weeks), in the mid-term (3–9 weeks), or later in the healing phase (> 10 weeks).
Results
A total of 78 patients with 85 titanium mesh augmentation were included with a mean follow-up of 1.2 years. The dehiscence was observed in 33 meshes (38.8%), which 51.5% within the first two weeks. In none of the cases was premature removal of the titanium mesh required following dehiscence. A statistically significant correlation between the occurrence of dehiscences and smoking behavior (p < 0.001) and the location of maxillary defect (p = 0.029) was observed. There was no significant correlation between the occurrence of dehiscences and gender (p = 0.160), periodontitis (p = 0.512), gingival type (p = 0.495), type of defect (p = 0,490), and incision technique (p = 0,354).
Conclusion
Dehiscence in augmentations using customized titanium meshes was primarily associated with smoking status and defect location in the maxilla. To achieve high success, early detection and treatment of dehiscence are crucial.
Split-dose Gastric Preparation Improves Dramatically the Degree of the Stomach Cleanliness of Magnet-Controlled Capsule Endoscopy
Authors: Sun Tingji , Zhang Yali , Cheng Chunsheng 
Published: August 2024

Abstract

The current standard preparation method produces poor cleanliness outcomes of magnetically controlled capsule. Inspired by the efficacy of a split-dose preparation in colonoscopy, the aim of this study was to examine the efficacy of a split-dose preparation for stomach cleanliness. Patients who received magnetic control capsule gastroscopy (enrolled August 2020 to December 2021) were divided into split-dose preparation group and singular-preparation group, Preparation outcomes, Gastric fluid turbidity and mucus/foam volume in the stomach were compared between the groups. A greater mean intragastric cleanliness score was obtained for the split-dose group (8.45 ± 1.76) and the singular-dose group (7.0 ± 1.0; P = 0.01). Outcomes were obtained in 14.3% and 7.1% (Excellent P > 0.05), 57.1% and 24.2% (Good,P < 0.05), 28.6% and 68.5% (Poor,P < 0.05), respectively. Split-dose preparation can improve stomach cleanliness.
Long non-coding RNA C20orf56 as predictor for response to neoadjuvant CCRT and survival rates of rectal cancers
Authors: Chen Chih-I , Yeh Cheng-Fa , Yang Ching-Chieh , Lee Sung-Wei , Tian Yu-Feng , Kuo Yu-Hsuan , Wu Li-Ching , Chien Yi-Che Chang , Chang I-Wei 
Published: August 2024

Abstract

Introduction: Colorectal cancers are the third most prevalent malignant neoplasms and the second leading cause of cancer mortality globally. The introduction of neoadjuvant concurrent chemoradiotherapy (CCRT) for patients with resectable rectal cancer not only improves survival rates but also increases the likelihood of curative surgeries. C20orf56, a long non-coding RNA, has been extensively documented as a tumor suppressor across various cancer types, participating in numerous cellular processes. However, no comprehensive evaluation has been conducted on the relationship between C20orf56 expression, response to neoadjuvant CCRT, and survival in patients with rectal cancer.
Materials and Methods: A comparative analysis of gene expression profiles from the transcriptomic dataset (GSE35452) identified C20orf56 as the most significantly up-regulated lncRNA. Tumor samples were collected from 343 primary rectal cancer patients who underwent neoadjuvant CCRT followed by surgical resection. The expression level of C20orf56 was semi-quantitatively assessed using in situ hybridization. Subsequently, statistical analyses were conducted to examine the relationship between C20orf56 expression, various clinicopathological features, and survival outcomes.
Results: Decreased expression of C20orf56 showed significant correlations with less advanced post-treatment tumor invasiveness, negative post-treatment nodal metastasis, absence of vascular invasion and perineural invasion, and improved response to neoadjuvant CCRT (all p ≤ 0.024). Diminished expression of C20orf56 was associated not only with favorable disease-specific survival (DSS), local recurrence-free survival (LRFS) and metastasis-free survival (MeFS) (all p < 0.0001) in univariate analysis but also functioned as an independent predictor signifying enhanced clinical outcomes, including DSS, LRFS, and MeFS (all p < 0.001).
Conclusion: C20orf56 may play a significant role in rectal cancer progression and response to neoadjuvant CCRT, serving as a novel prognostic factor.
Investigation of the Effects of Boric Acid Against Post Operative Testicular Adhesion Caused by Experimental Laporotomy in Rats
Authors: BOLAT İsmail , BOLAT Merve , KİLİÇLİOĞLU Metin , OKUR Sıtkıcan , GÖLGELİ Ayşe , GÖZEGİR Berrah , ÇOMAKLI Selim , YILDIRIM Serkan , SAĞLAM Yavuz Selim , WARDA Mohamad 
Published: August 2024

Abstract

Post-operative intra-abdominal adhesions, significantly affecting testicular tissue, are a prevalent and serious complication following laparoscopic surgery. This study investigated the efficacy of boric acid, known for its antioxidant, anti-inflammatory, and anti-apoptotic properties, in preventing post-operative testicular adhesions. Forty rats, weighing between 230 and 290 grams, were divided into four groups: control, laparoscopy (LA), boric acid (BA), and LA + BA. Following laparoscopic surgery, BA treatment was administered for seven days. Adhesion scores were markedly higher in the LA group, whereas the LA + BA group exhibited a significant reduction in adhesion scores. Testicular tissues were analyzed using immunohistochemistry, immunofluorescence, Masson's trichrome staining, biochemical assays, and histopathological evaluation. In the LA group, malondialdehyde (MDA) levels increased while superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels decreased; these parameters normalized with BA treatment. Additionally, the LA group exhibited reduced levels of IL-10, Bcl-2, Kisspeptin-1, and GnRH, alongside elevated levels of inflammatory markers IL-1β, IL-6, TNF-α, JNK, BAX, and Caspase 3. Boric acid treatment significantly restored these levels to normal. In conclusion, oxidative stress, inflammation, and apoptosis in testicular tissues were associated with post-operative testicular adhesions. Boric acid demonstrated potential as an anti-adhesive agent, reducing testicular adhesions and normalizing biochemical and histological parameters following laparoscopic surgery.