bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Concentration of 5‐S‐Cysteinyldopa in Pediatric Patients with Giant Pigmented Nevi
Authors: Hanawa Yoko , Wakamatsu Kazumasa , Ikeda Shigeo 
Published: April 2015

AbstractThe serum concentrations of 5‐S‐cysteinyldopa (5‐S‐CD) in pediatric patients with giant pigmented nevi (GPN) were investigated and compared with those of pediatric patients with small‐ or medium‐sized congenital nevi (CN), or non‐melanocytic benign skin tumor (control group). Serum 5‐S‐CD levels in the GPN group (N=21), particularly in patients less than 5 years old, were significantly higher(highest concentration 38.4, mean ± S.D. 16.6 ± 9.4 nmol/L) than those in the CN (N=22) or control groups (N=26). Serum 5‐S‐CD levels in the CN group were not significantly different from those in the control group. There was a significant correlation between the serum 5‐S‐CD level and size (surface area) of the GPN. There was a significant inverse correlation between the serum 5‐S‐CD level and age in the CN and control groups, but not in the GPN group. Serum 5‐S‐CD levels were transiently elevated immediately after treatment of patients with GPN with a combination of skin abrasion and cryotherapy with solid carbon dioxide.These results suggest that serum 5‐S‐CD levels in the GPN group reflected the 5‐S‐CD derived from GPN, particularly in patients less than 5 years old. This indicates that melanogenesis may be accelerated in infant patients in the GPN group.
Acne Fulminans Coexisting with Pyoderma Gangrenosum‐like Eruptions and Posterior Scleritis
Authors: Kurokawa Shigeko , Tokura Yoshiki , Nham Nguyen Xuan , Sudoh Harumi , Wakita Hisashi , Furukawa Fukumi , Takigawa Masahito 
Published: April 2015

AbstractA 30‐year‐old Japanese man presented with an erythematous, papulonodular eruption on his face and upper trunk, which rapidly progressed into severely exuberant, ulcerative lesions with a confluent tendency. His other symptoms included a high fever and general malaise. Complete blood cell counts showed neutrophilia with increased percentages of myelocytes and metayelo‐cytes. The systemic administration of corticosteroids improved his skin lesions, leaving scars and milia. During tapering of corticosteroids, however, the patient developed posterior scleritis of his eyes and pyoderma gangrenosum‐like eruptions on the lower legs. Our patient's history suggests that pyoderma gangrenosum‐like eruptions and posterior scleritis are disorders associated with acne fulminans.
Macrophage Colony‐Stimulating Factor (M‐CSF) Inhibits the Decrease in the Amount of rRNA and IAβ mRNA in Cultured Epidermal Langerhans Cells of the Mouse
Authors: Koyama Yoh‐ichi , Marunouchi Tohru 
Published: April 2015

AbstractMacrophage colony‐stimulating factor (M‐CSF) is a keratinocyte‐derived cytokine whose function in skin is not completely clarified. We investigated its effects on Langerhans cells by examining the amount of IAβ mRNA, β‐actin mRNA and rRNA per cell, and compared them with the effects of other cytokines such as granulocyte/macrophage colony‐stimulating factor (GM‐CSF) and tumor necrosis factor‐α (TNF‐α). After culture for 24 h in the absence of exogenous cytokines, rRNA in Langerhans cells decreased steeply while β‐actin mRNA increased. IAβ mRNA also decreased sharply. These decreases in the amount of rRNA and IAβ mRNA were limited when cytokines were added to the culture medium (in order of efficiency M‐CSF>GM‐CSF>TNF‐α), but M‐CSF was less potent than GM‐CSF in up‐regulating β‐actin mRNA (GM‐CSF>M‐CSF, TNF‐α). The effect of M‐CSF, but not that of GM‐CSF, was restricted by simultaneous treatment of cells with TNF‐α. None of these effects engendered a change in the viability of the Langerhans cells in a 24‐hr culture. Reverse‐transcribed polymerase chain reaction analysis demonstrated that c‐fms, the gene of the M‐CSF receptor, was expressed in Langerhans cells, implying the physiological importance of M‐CSF in vivo. A protein kinase C activator, TPA, up‐regulated the amount of IAβ mRNA, while a protein kinase C inhibitor, H‐7, suppressed the effects of all three cytokines. These results suggest that M‐CSF, in conjugation with TNF‐α and GM‐CSF, plays an important role in controlling the physiological state of Langerhans cells, probably through the activation of protein kinase C.
Effects of Transforming Growth Factor β1 in the Hair Cycle
Authors: Mori Osamu , Hachisuka Hiroshi , Sasai Yoichiro 
Published: April 2015

AbstractHair follicle growth is thought to be regulated by a complex interplay of stimulatory and inhibitory signals. From among such signals, we examined the effects of transforming growth factor β1 (TGFβ1) on the murine hair growth cycle.Quantitation of TGFβ1 per wet tissue weight was performed at different stages of the hair cycle. TGFβ1 was extracted with 10 mM HCl, and its level was measured with ELISA. The level of TGFβ1 did not change markedly during the hair cycle. Immunohistochemical observations revealed that hair follicle cells and epidermal cells were negative for TGFβ1 at all stages of the hair cycle. Mast cells in the dermis were positive throughout the cycle. To confirm whether TGFβ1 inhibits the proliferation of hair follicles, we injected it subcutaneously. TGFβ1 inhibited the proliferation of hair follicles. Our observations suggest that TGFβ1 is at least partly responsible for regulating hair follicles as a negative growth factor.
Expression of E‐Cadherin in Skin Carcinomas
Authors: Tada Hideyuki , Hatoko Mitsuo , Muramatsu Tsutomu , Shirai Toshihiko 
Published: April 2015

AbstractIn this study, we investigated the expression of E‐cadherin in 31 cases of human skin carcinoma including basal cell carcinoma (BCC), squamous cell carcinoma (SCC), Paget's disease, Bowen's disease (invasive type), and trichilemmal carcinoma, by immunohistochemical staining using a monoclonal antibody specific for E‐cadherin. Similar to the E‐cadherin expression in normal epidermis, E‐cadherin was strongly expressed in all samples of BCC on the cell borders, whereas marked decrease or loss of E‐cadherin expression was found in the tumor cells of SCC, Paget's disease, and Bowen's disease (invasive type). On the other hand, E‐cadherin expression of trichilemmal carcinoma was slightly reduced. Considering the clinical and histological features of these skin carcinoma, the reduction of E‐cadherin expression is considered to be associated with the invasion and metastasis of human skin carcinoma.
Bacteroides fragilis Pyomyositis in a Patient with Multiple Myeloma
Authors: Katagiri Kazumoto , Shibuya Hiromi , Takayasu Susumu 
Published: April 2015

AbstractPyomyositis is a bacterial infection with abscess formation affecting large skeletal muscles. It is predominantly caused by Staphylococcus aureus. The disease is common in tropical areas, but rare in temperate climates. We report a patient with multiple myeloma who developed a giant elastic tumor on the right thigh and a hen egg‐sized tumor on the right upper arm. MR imaging revealed cystic spaces in the femoral quadriceps and brachial biceps muscles. A large amount of pus with foul smell was removed by incision, drainage and aspiration of the two tumors. The lesions were successfully treated with intravenous administration of antibiotics. Repeated bacterial cultures yielded only Bacteroids fragilis. To our knowledge, this is the first report of pyomyositis due to Bacteroides fragilis.
A Case of Tick Bite from Amblyomma testudinarium on the Glans Penis
Authors: Yamada Yoshitaka , Dekio Satoshi , Jidoi Joji , Isobe Akio , Shiwaku Kuninori , Yamane Yosuke 
Published: April 2015

AbstractA tick which bit the glans penis of a 40‐year‐old Japanese man was surgically excised together with the skin where the bite occurred. It was identified as Amblyomma testudinarium through the taxonomical investigation of its morphological characteristics. To our best knowledge, our patient is the sixth tick bite from this species of tick recorded in the Chugoku District of Japan.
Measurement of Skin Elastic Properties with a New Suction Device (II): Systemic Sclerosis
Authors: Ishikawa Takeshi , Tamura Taeko 
Published: April 2015

AbstractWe previously reported the skin elastic properties of normal subjects as measured by a newly developed suction device (6). In the present report, we examined the skin elastic properties of 62 patients with systemic sclerosis (SSc), clinically diagnosed as limited or diffuse type. We measured the skin elastic properties on the forearm and chest and investigated correlations with disease type and clinical skin score. Patients with the diffuse type SSc showed significantly lower values than those with the limited type SSc or normal subjects in distention and retraction ability at the chest and forearm.This suction device is clinically useful for the objective evaluation of skin elastic properties in diffuse systemic sclerosis.
Hereditary Onset of Multiple Seborrheic Keratoses: A Variant of Leser Trélat Sign?
Authors: Yamamoto Toshiyuki , Yokoyama Akiko 
Published: April 2015

AbstractA 79‐year‐old man in a cancer‐prone family developed disseminated seborrheic keratoses over his trunk and extremities over a few decades without rapid increase. His son, mother, and one of his brothers, who died of gastric cancer, also had numerous seborrheic keratoses for a long time. He had a biliary tract cancer. Histologic examination showed hyperproliferation of basaloid cells branching downwards, as if induced by surrounding stroma. No decrease of the number of seborrheic keratoses after surgical operation has been observed. Examination for the localization of epidermal growth factor receptor (EGF‐R) and HER‐2/neu oncoprotein by immunohistochemistry revealed positive staining on the epithelial strands branching downwards on the specimens of seborrheic keratoses. A different pattern of expression was demonstrated in normal seborrheic keratosis. These findings suggest that some unidentified growth factors may be involved in the induction of visceral and/or cutaneous neoplasms in this family.
Contact Dermatitis Due to Topical Steroids with Conceivable Cross Reactions between Topical Steroid Preparations
Authors: Oh‐i Tsunao 
Published: April 2015

AbstractSteroids are usually employed to inhibit inflammation. However, allergic contact dermatitis has been reported with topical steroids. We have experienced contact dermatitis due to active ingredients of topical steroids in 11 patients. Therefore, we make it a rule to carry out the patch test with as many topical steroids as possible. Five patients were confirmed to be positive to several corticosteroids in the patch test. Papers on patients allergic to steroids indicate that some patients react to several. It is difficult to evaluate whether the reaction is caused by multisensitivity or by cross reaction. However, even if steroids differ in type, they may often cause cross reactions when they have a common chemical structure. Considering this chemical structure, we tried to classify steroids reported to cause allergy in patients into the following four types:
The type recognizing betamethasone and/or dexamethasone. Regardless of whether the methyl group at the C‐16 position of the D ring is on the α or β side, the size of the blocking group of the hydroxy residue seems to be associated with the reaction.
The type in which a chlormethylketone group at the C‐17 position of the D ring seems to be associated with the reaction.
The type in which a cis‐diaxial‐diol side chain in the α side of C‐16 and C‐17 positions of the ring D or its ketal blocking group seems to be associated with the reaction.
The type in which the size of the ester protecting the hydroxy group at the C‐17α position seems to be associated with the reaction.
In patients showing a reaction, there may be receptors recognizing each steroid. If the steroid administered is changed, the receptor appears to recognize the changed steroid in a relatively short period. Thus the receptor may not be constant.
Alteration of Human Melanoma Gangliosides by IFN‐γ, IL‐2, and IL‐4
Authors: Ando Iwao , Komine Mayumi , Otsuka Fujio , Kukita Atsushi 
Published: April 2015

AbstractIn lesions of malignant melanoma, melanoma cells are exposed to various cytokines produced by inflammatory reactions. As a result, transformation of melanoma cells is expected to occur. We studied alterations in human melanoma cell line ganglioside composition after exposing melanoma cell lines to interferon (IFN)‐γ, interleukin (IL)‐2, and IL‐4 by biochemical methods. IFN‐γ increases the ratio of a‐series gangliosides and the ratio of GM3/GD3. This suggests an alteration of immunoreactivity, a decrease in ganglioside sialyltransferase II activity, and an decrease in the malignant character of these cells. The alteration of the ganglioside profile varied among cytokines and cell lines. The progression of malignant melanoma may be influenced by reciprocal interactions between the melanoma cells and the host immune system.
Tissue Distribution of a Melanoma‐Associated Antigen Immunogenic in Patients with Melanoma as Analyzed by Polyclonal Antibodies to Recombinant Peptide Antigen
Authors: Kako Satoru , Hayashibe Kazuhito , Ichihashi Masamitsu 
Published: April 2015

AbstractThis study aimed to detect in vivo expression of human melanoma‐associated antigen D‐1, which was identified by screening an expression cDNA library constructed from mRNA extracted from cultured melanoma cells with sera from patients with melanoma. The tissue distribution of D‐1 antigen was then analyzed. Murine anti‐D‐1 recombinant peptide polyclonal antibodies were raised by immunization of in vitro synthesized D‐1 peptide against Balb/c mice and applied immunohistochemically on paraffin‐embedded tissue specimens. D‐1 antigen was found to be restrictedly expressed on melanoma cells, but not on normal melanocytes, adjacent keratinocytes, fibroblasts, lymphocytes and adnexal structures of skin. The reactivities of anti‐D‐1 antibodies did not correlate with histogenesis of the lesions, their ability to produce melanin, and/or their primary or metastatic nature. There was no positive reactivity of anti‐D‐1 antibodies with other skin tumors, including squamous cell carcinoma, basal cell epithelioma, seborrheic keratosis, and nevus cell nevus. Further, cytoplasmic expression of D‐1 antigen in melanoma cells was observed only in a certain subgroup of patients with melanoma. This indicates that the cell surface expression of D‐1 peptide requires specific transporting proteins, such as HLA molecules.
Relationship between N‐Acetyltransferase (NAT) Activity and Liver Protoporphyrin Level in Experimental Porphyria
Authors: Watanabe Masahisa , Irifune Hiroko , Ohgami Taro , Nonaka Shigeo 
Published: April 2015

AbstractThe results of our previous studies demonstrated that isonicotinic acid hydrazide (INH) can aggravate griseofulvin (GF)‐induced protoporphyria in mice. To elucidate this phenomenon, we studied the relationship between liver protoporphyrin (PP) levels and N‐acetyltransferase (NAT) activity, which is known to be a major catabolic enzyme of INH metabolism in the liver.The results revealed a significant correlation between liver PP levels and NAT activity in the mice fed 0.1% GF and 0.05% INH. In this group, mice with high NAT activity developed severe protoporphyria. There was no correlation, however, between liver PP levels and NAT activity in the mice fed GF alone or INH alone.The result suggested that INH metabolites formed by NAT enhance the hepatotoxicity of GF in mice.
Topical Spironolactone Reduces Sebum Secretion Rates in Young Adults
Authors: Yamamoto Ayako , Ito Masaaki 
Published: April 2015

AbstractThe effects of topically applied spironolactone on the sebum secretion rates (SSR) of young adults were investigated. SSR was expressed as the ratio of wax esters/[cholesterol + cholesterol esters] (WE/[C + CE]) and the amount of sebaceous lipids (squalene, triacylglycerol and wax esters). Topical spironolactone 5% gel applied to the right cheeks of the subjects produced a significant reduction in the SSR at 12 weeks (4 weeks after termination of application), but not at 8 weeks (the end of treatment). Untreated “control” areas (the left cheeks of the subjects) showed no significant change during the study. None of the subjects experienced skin rash or signs of local irritation. This results suggests that topical spironolactone may be effective in the treatment of acne patients with high SSR
Two Cases of Atypical Bullous Disease Showing Linear IgG and IgA Deposition in the Basement Membrane Zone
Authors: Kawahara Yoshie , Hashimoto Takashi , Watanabe Kyoko , Kurihara Seiichi , Matsuo Itsuro , Nishikawa Takeji 
Published: April 2015

AbstractPatients showing coexistent linear IgG and IgA deposition along the basement membrane zone on direct immunofluorescence have been described as either bullous pemphigoid, epidermolysis bullosa acquisita, linear IgA bullous dermatosis, or cicatricial pemphigoid, depending on the clinical features and laboratory findings. In the present report, we describe two cases showing atypical clinical features distinct from those of other known bullous diseases. No circulating antibodies were detected by indirect immunofluorescence of normal human skin. Indirect immunofluorescence of 1 M NaCl split skin revealed IgG and/or IgA antibodies reactive with the dermal side of the split. Immunoblotting of normal human epidermal and dermal extracts showed no apparent reactivity with known autoantigens. The results suggest that there may be a unique and distinct bullous disease with linear IgG and IgA deposition at the basement membrane zone.
Successful Treatment of Mucosal Melanosis of the Lip with Normal Pulsed Ruby Laser
Authors: Hanada Katsumi , Baba Takako , Sasaki Chiaki , Hashimoto Isao 
Published: April 2015

AbstractMelanosis of the lip is not rare in Orientals. Although it causes cosmetic problems, treatment of this pigmentary disorder is often neglected because a satisfactory tool for complete removal of mucosal melanosis has not yet been developed. The pulsed ruby laser, which has a wavelength of 694 nm and a short pulse duration of 1–2 msec, selectively damages pigmented cells in the skin. In this study, six Japanese patients with labial melanosis, manifested as labial lentigo and Peutz‐Jeghers syndrome, were successfully treated with the pulsed ruby laser. The therapy achieved rapid results without producing changes in mucosal texture or recurrence after operation
Patent Odontogenic Sinus Tract Draining to the Midline of the Submental Region: Report of a Case
Authors: Urbani Carlo Enrico , Tintinelli Roberto 
Published: April 2015

AbstractWe report a case of a 65‐year‐old woman with a cutaneous sinus tract located on the midline of the submental region secondary to a periapical abscess of the right lateral mandibular incisor. The lesion was nodulocystic and chronically drained purulent fluid. Previous topical and systemic treatments were uneffective. Radiologic examination of the mandible demonstrated diffuse radiolucency involving the apices of four affected incisors. A further radiologic sinogram revealed both the exact origin and the high grade patency of the fistolous tract. Appropriate conservative endodontic therapy led to quick resolution of the sinus tract within sixteen days. In the presence of a single chronic suppurative or nodulocystic lesion of the face, it is always useful to perform a radiologic evaluation of the maxillary and mandibular regions to promptly exclude a possible odontogenic background.
Extramammary Paget's Disease: A Report of 22 Cases in Chinese Males
Authors: Chang Yun‐Ting , Liu Han‐Nan , Wong Chu‐Kwan 
Published: April 2015

AbstractExtramammary Paget's disease (EMPD) is more frequently seen in Caucasian females than in males (3.2:1 female: male ratio). During the past 14 year period, we have collected 22 patients, all Chinese males, with EMPD. They presented with eczema‐like lesions in the early stages in the genital or perianal regions. Histological sections showed Paget cells within the epidermis or skin appendages and even within the dermis. No underlying adnexal carcinoma or adjacent internal carcinoma could be detected after thorough examinations. Mode of therapy and outcome are presented. EMPD seems to affect more males than females in Orientals. The incidence of concomitant malignancy in Chinese male patients with genital Paget's disease seems to be much lower than that in Caucasians. However, if EMPD involves the glans penis or perianal area, a search for internal malignancy is still warranted.
Coexistence of Plaque‐Type Blue Nevus and Congenital Melanocytic Nevi
Authors: Aroni Kyriaki , Georgala Sophia , Papachatzaki Elektra , Liossi Anna , Davaris Panagiotis 
Published: April 2015

AbstractA rare case of development of plaque‐type blue nevus adjacent to congenital nevi in a 44‐year‐old woman is presented.
Antiphospholipid Syndrome Associated with Progressive Systemic Sclerosis
Authors: Chun Woo Hyung , Bang Dongsik , Lee Soo Kon 
Published: April 2015

AbstractWe report a case of secondary antiphospholipid syndrome (APS) occuring in a progressive systemic sclerosis (PSS) patient who took herbal medication.Clinical findings compatible with APS included positive IgM anticardiolipin antibody (ACL), thrombocytopenia, and obstruction of the left radial artery on digital subtraction angiography (DSA), Clinical findings compatible with PSS included sclerodactyly and digital ulcers, Raynaud's phenomenon, pulmonary fibrosis and pulmonary hypertension, proteinuria and renal mesangial reaction, and myocarditis.
A Case of Stasis Papillomatosis Associated with Psoriasis Vulgaris
Authors: Sato Toshihiro , Katagiri Kazumoto , Itami Satoshi , Takayasu Susumu 
Published: April 2015

AbstractA markedly obese, 41‐year‐old Japanese man who had suffered from psoriasis vulgaris for several years visited us with elephantiasis‐like swelling of his lower legs of three months' duration. His right lower leg showed marked papillomatosis with thick scales, and the left lower leg was eroded and papillomatous. Although direct lymphography of his lower extremities showed no abnormality, indirect lymphography revealed local lymphatic damage in the involved skin. Histological examination showed hyperkeratosis, marked papillomatosis, proliferation of capillaries in the upper dermis, and lymphectasia in the lower dermis. The lesions were much improved by washing and topical use of corticosteroids for two months. It was suspected that obesity and the preceding psoriatic lesions caused local lymphatic disturbances, followed by the development of stasis papillomatosis.
Serum Levels of Eosinophil Cationic Protein Reflect the State of In vitro Degranulation of Blood Hypodense Eosinophils in Atopic Dermatitis
Authors: Miyasato Minoru , Tsuda Shingo , Nakama Takekuni , Kato Keiko , Kitamura Naohisa , Nagaji Junko , Sasai Yoichiro 
Published: April 2015

AbstractIn patients with atopic dermatitis (AD), serum levels of eosinophil cationic protein (ECP) have been shown to be a good reflector of disease severity. To elucidate what serum levels of ECP actually reflect, ECP levels in serum and plasma and cytological aspects of blood eosinophils were examined in AD patients (n=27) and compared to healthy subjects (n=12). Significantly elevated levels of serum ECP were noted in AD patients, while plasma ECP were uniformly recorded at nadir levels in both AD patients and normal subjects. In addition to blood eosinophilia, AD patients had significantly increased numbers of hypodense eosinophils (HEo) with morphological characteristics consistent with an activated state. Serum ECP levels strongly correlated with HEo numbers rather than with total eosinophil counts. These results indicate that elevated levels of serum ECP may be a consequence of in vitro degranulation of “activated” HEo, not of ECP supplementation from lesional skin. In addition, the dynamic correlations of eosinophil‐associated parameters (total eosinophil counts, HEo numbers, and serum ECP levels) with AD severity suggest that inflammatory events in lesional skin may be involved in causing not only eosinophilopoiesis in bone marrow, but also development of HEo in the periphery, whose degree in turn may be mirrored in the levels of serum ECP in vitro.