bluuebunny/crossref_metadata_2025_split huggingface dataset:
Giant mucinous liposarcoma of the abdominal cavity: A case report
Authors: Li Yi-Ming , Zhu Hai-Hong , Wang Xiang-Qian , Shi Meng-Zhen , ShangGuan Chao-Liang 
Published: August 2024

Rationale:
Mucinous liposarcoma myxoid liposarcoma is a malignant mucoid soft tissue tumor derived from undifferentiated stromal cells in perivascular, subbody cavity and intermuscular space, and composed of cells at different stages of differentiation from preadipocytes to mature cells. In rare cases, it may change from lipoma malignancy. The main manifestations is painless mass, relatively slow growth, the course can last decades, the prevalence of liposarcoma in the population is 14% to 18%, mainly in adults, male prevalence is higher than women, but not significant. The main good hair part is the thigh, have mucinous sex, high differentiation type, dedifferentiation type, polymorphic type. Clinical diagnosis is difficult, and there are no obvious symptoms in the early stage, so the diagnosis should be combined with B ultrasound, MRI, CT, and other auxiliary examinations. The gold standard is pathological examination. In December 2023, our department admitted a patient with a mucinous abdominal mass. The report is as follows.

Patient concerns:
Does liposarcoma metastasize? Is any chemotherapy required after surgery? Will it ever relapse in the future? What is the survival period after surgery?

Diagnosis:
Mucinous liposarcoma.

Interventions:
Surgical resection of the sarcoma.

Results:
The nodule sample was 33 * 28 * 13 cm, with complete capsule, gray and yellow sections, fine texture, soft, gray, red, grayish, and yellow mucoid nodules in some areas, and the maximum diameter of the nodules was 21cm. Immunohistochemistry was: CD34 (+), CDK 4 (+), CK (−), Desmin (weak +), Ki67 (index 5%), MDM 2 (−), p16 (weak +), S-100P (+), Vimentin (+), BCL-2 (+). He was also sent to the Department of Pathology of Peking Union Medical College Hospital for consultation with Professor Lu Zhaohui, whose consultation opinion was in line with myxoliposarcoma.

Conclusion:
Retroperitoneal liposarcoma is a common retroperitoneal tumor, but it is relatively rare in clinical practice; the overall morbidity is low, mainly manifested as abdominal pain and abdominal distension, abdominal distension, and a long course of disease; it is not sensitive to radiotherapy and chemotherapy, and should be closely follow up by CT examination to understand the recurrence and metastasis.
Beliefs, preferences, and acceptance of personal protective equipment for a shock room team in a tertiary care center
Authors: Ott Matthias , Hein Anna Sophie , Krohn Alexander , Jaki Christina , Dengler Florian , Schilling Tobias , Heymer Johannes 
Published: August 2024

Background:
During coronavirus disease of 2019 pandemic a standard usage of personal protective equipment (PPE) in healthcare was mandatory, while actually the usage of PPE is currently decreasing. This raises the question about the further use of PPE in the clinical setting because healthcare workers (HCW) are at greater risk of being infected with SARS-CoV-2 than the general population. The primary objective of this study is to determine the proportion of shock room team members approving the further use of PPE including a FFP2 respirator in simulation training and reality. The secondary objectives are to describe the expertise and difficulties faced while using PPE in the shock room care.

Methods:
Fifty-four HCW participated in a shock room simulation training at a large urban tertiary care hospital in Germany, utilizing a PPE comprising an FFP2 mask, gloves, goggles, and gown. Subsequently, participants completed an online questionnaire featuring 15 questions presented on a 5-point Likert scale or as multiple-choice questions with predefined answers.

Results:
Sixty-eight point five percent of our participants voted for an established standard PPE in shock room care. The largest fraction of our participants (40.7%) favors a standard PPE consisting of FFP2 mask, gown, and gloves. Less HCW (31.5%) want to wear PPE in shock room simulation training. Except for goggles we could not detect relevant difficulties faced while using PPE in the shock room environment. Incorrect use of PPE was observed in 14.8%.

Conclusion:
A majority of our participants favored a standard PPE including a FFP2 respirator in shock room care. In addition, we recommend the use of PPE in shock room simulation training, while further awareness of and training in proper use of PPE seems to be necessary to reduce risk of infectious diseases for HCW.
The impact and mechanism study of Sijunzi decoction and Rg1 on proliferation and differentiation of human umbilical cord mesenchymal stem cells: An experimental study
Authors: Lu Yafei , Ma Chuanxue , Zhang Yajie , Zhu Weina , Huangfu Shaohua , Zhou Ying , Zhou Chungen , Qin Fuhao , Wang Jianmin , Li Ming , Jiang Bin 
Published: August 2024

Background:
Previous researches have demonstrated that the traditional Chinese medicine could therapeutically treat inflammatory and hypoxic diseases by enhancing the functionality of mesenchymal stem cells. However, its mechanism was not yet clear. This research aimed to investigate the impact of the traditional Chinese medicine Sijunzi decoction and its herb monomer ginsenoside Rg1 on the proliferation and differentiation of human umbilical cord mesenchymal stem cells (hUC-MSCs) and explore the underlying mechanisms.

Methods:
Different concentrations of Sijunzi decoction and Rg1 were applied to differentiating induced hUC-MSCs. The CCK-8 test was utilized to evaluate cell proliferation activity and identify suitable drug concentrations. Alizarin Red staining was employed to detect the formation of calcium nodules, and Oil Red O staining was used to assess the formation of lipid droplets. PCR was utilized to examine gene expression related to osteogenic differentiation, adipogenic differentiation, and the HIF-1α signaling pathway in hUC-MSCs. Western blot analysis was conducted to evaluate protein expression in osteogenic differentiation and HIF-1α. ELISA was performed to measure HIF-1α signaling factors and inflammatory cytokine expression. Biochemical assays were used to assess changes in oxidative stress indicators.

Results:
The Sijunzi decoction and Rg1 both demonstrated a dose-dependent promotion of hUC-MSC proliferation. The Sijunzi decoction significantly increased the expression of genes and proteins relevant to osteogenesis, such as osterix, osteocalcin, RUNX2, and osteopontin, and activated the HIF-1α pathway in hUC-MSCs. (P < .05). Similar effects were observed at the gene level after treatment with Rg1. Simultaneously, Sijunzi decoction significantly reduced the secretion of pro-inflammatory cytokines TNF-α, IL-6, and IL-1β, while increasing the secretion of the anti-inflammatory cytokine IL-10 during osteogenic differentiation (P < .05). Moreover, Sijunzi decoction lowered oxidative stress levels and enhanced the antioxidant capacity of hUC-MSCs during osteogenic differentiation (P < .05). However, the impact of Sijunzi decoction on hUC-MSCs toward adipogenic differentiation was not significant (P > .05).

Conclusion:
Sijunzi decoction promotes the proliferation and osteogenic differentiation of hUC-MSCs, potentially through the activation of the HIF-1α signaling pathway and by modulating the microenvironment via reducing inflammation and oxidative stress levels. Rg1 might be involved in this process.
A case report of iliopsoas abscess and literature review
Authors: He Wenyu , Yuan Ye , Huang Jihua 
Published: August 2024

Rationale:
Iliopsoas abscess is a rare acute medical condition. It usually occurs because of the spread of infection from adjacent structures and hematogenous spread. Clinical features include fever, backache, radiating nerve root pain, and leg weakness. When sepsis occurs, prompt recognition is required to initiate appropriate antimicrobial therapy and surgical drainage.

Patient concerns:
A 65-year-old male presented to the outpatient department with a 2-day history of lower back, hip, and leg pain, for which analgesics were administered. During hospitalization, he experienced deterioration, becoming febrile, hypoxic, hypotensive, tachycardiac, and delirious.

Interventions:
The patient was then intubated and ventilated. His family reported an additional history of acupuncture for back pain, which sustained an inflamed wound on his right forearm. Abdominal computed tomography was performed, which confirmed bilateral iliopsoas abscess without involvement of intra-abdominal organs. A preliminary report of blood culture revealed Gram-positive cocci. Echocardiography showed vegetation on the aortic valve, and moderate aortic regurgitation was sustained. He was started on vancomycin along with piperacillin–tazobactam. Ultrasound-guided percutaneous drainage was inserted into the bilateral abscess. Pus and blood yielded methicillin-sensitive Staphylococcus aureus. He remained septic. The repeat computed tomography showed the right abscess enlarged. A repeated echocardiogram showed that the vegetation increased. Further incision and surgical drainage were performed with continuous wash-out.

Outcome:
His condition improved after management and he was discharged to a regional hospital for ongoing care.

Conclusion:
Prompt diagnosis and surgical treatment are essential to improve patient outcomes. The unique aspect of this case is the persistence of the methicillin-sensitive Staphylococcus aureus infection. Centralized surgical services are pivotal in conjunction with robust antimicrobial regimens.

Lesson:
This case reinforces the importance of high clinical suspicion of an unknown source of sepsis.
Bioinspired Bimetallic Ions Functionalized MOF SAzyme Nanocomposites for Synergistic Ferroptosis/Cuproptosis-Enhanced Immune Checkpoint Therapy
Authors: Yang Linjiao , Pan Mengmeng , Hu Haofan , Liu Furong , Jiang Ming , Ning Shangwu , Chen Xiaoping , Zhang Zhanguo , Yu Xu , Xu Li 
Published: August 2024

AbstractImmune checkpoint blockade (ICB) generates sustained responses in immunogenic cancers, but its effectiveness is limited in tumors lacking immune activity. Here, we construct a bioinspired bimetallic ions functionalized nanoscale metal-organic framework (NMOF) single-atom nanozyme (SAzyme) loaded with doxorubicin (Dox) (NMOF-Fe/Cu-Dox nanocomposite) to effectively trigger anti-tumor immune responses while addressing the immunosuppressive tumor microenvironment (TME). The NMOF-Fe/Cu-Dox nanocomposite has been demonstrated to efficiently reverse the TME by generating reactive oxygen species and oxidizing glutathione. Camouflaging NMOF-Fe/Cu-Dox nanocomposites with bioinspired cancer cell membrane (NMOF-Fe/Cu-Dox@M) enables its navigation to the tumor region through homologous targeting. The highly efficient uptaken by cancer cells selectively induced synergistic ferroptosis and cuproptosis in these cells. Furthermore,in vitroandin vivoexperiments demonstrate that the ferroptosis in cancer cells can polarize tumor-associated macrophages (TAMs) towards anti-tumoral M1 phenotype and significantly diminish pro-tumoral M2 phenotype. We find that NMOF-Fe/Cu-Dox@M could induce the ferroptosis of M2 macrophages, while no effect to M1 macrophages. In addition, a significant increase of anti-tumor infiltrating CD8+T cells, while a remarkable decrease of CD4+regulatory T cells were observed. These findings suggested that NMOF-Fe/Cu-Dox@M could efficiently modulate TME, enhance tumoricidal immunity and elevate the therapeutic efficiency of ICB. Moreover, the combination of NMOF-Fe/Cu-Dox@M with αPD-1 effectively eradicated hepatocellular carcinoma cellsin vivo, outperforming the use of either NMOF-Fe/Cu-Dox@M or αPD-1 alone. In summary, our study presents a therapeutic strategy that leverages coordinated ferroptosis and cuproptosis with therapeutic efficiency of ICB, underscoring the promise of combined chemoimmunotherapy.
Association between the choroid plexus volume and cognitive function in community-dwelling older adults without dementia: A population-based cross-sectional analysis
Authors: Hidaka Yosuke , Hashimoto Mamoru , Suehiro Takashi , Fukuhara Ryuji , Ishikawa Tomohisa , Tsunoda Naoko , Koyama Asuka , Honda Kazuki , Miyagawa Yusuke , Yoshiura Kazuhiro , Yuuki Seiji , Kajitani Naoto , Boku Shuken , Ishii Kazunari , Ikeda Manabu , Takebayashi Minoru 
Published: August 2024

ABSTRACTBackgroundChoroid plexus (CP) volume increase may be associated with cognitive decline in older individuals without dementia. In this study, we aimed to clarify whether CP volume can be an imaging marker of cognitive decline in older adults, verify its association with cognitive decline in older individuals compared with brain parenchymal and CSF volumes, and explore the factors associated with the CP volume.MethodsWe measured the CP volume, brain parenchyma, and cerebrospinal fluid (CSF) spaces associated with disproportionately enlarged subarachnoid space hydrocephalus (DESH), an imaging feature of normal-pressure hydrocephalus, in community-dwelling individuals aged ≥65 years without dementia.ResultsIn 1,370 participants, lower Mini-Mental State Examination (MMSE) scores were significantly associated with higher CP volume, even after adjusting for DESH-related CSF space and brain parenchymal volume. CP volume was more strongly associated with MMSE scores than DESH-related CSF space and brain parenchymal volume. History of smoking, white matter hyperintensity, enlarged perivascular spaces, age, body mass index, and diabetes mellitus were associated with increased CP volume.ConclusionsIn community-dwelling older individuals without dementia, CP volume is associated with cognitive decline independent of the brain parenchyma and CSF volumes and may be one of the most sensitive imaging markers of cognitive decline. Our findings emphasize the importance of investigating CP volume increase to maintain cognitive function in older individuals. Further longitudinal studies are required.WHAT IS ALREADY KNOWN ON THIS TOPICChoroid plexus (CP) volume increase has been associated with cognitive decline in older individuals without dementia; however, the influence of volumes of cerebrospinal fluid (CSF) and brain parenchyma in this association remains unexplored. The extent to which CP volume is involved in cognitive decline in older adults compared with brain parenchymal and CSF volumes is unclear.WHAT THIS STUDY ADDSCP volume is associated with cognitive decline in older individuals independent of the volumes of CSF and brain parenchyma. CP is one of the structures most closely related to cognitive impairment in older individuals without dementia.HOW THIS STUDY MIGHT AFFECT RESEARCH, PRACTICE OR POLICYOur study provides a reference for investigating CP volume increase to maintain cognitive function in older individuals.
Engineered Nanotopographies Induce Transient Openings in the Nuclear Membrane
Authors: Sarikhani Einollah , Patel Vrund , Li Zhi , Meganathan Dhivya Pushpa , Rahmani Keivan , Sadr Leah , Hosseini Ryan , Visda Diether , Shukla Shivani , Naghsh-Nilchi Hamed , Balaji Adarsh , McMahon Gillian , Chen Shaoming , Schöneberg Johannes , McHugh Colleen A. , Shi Lingyan , Jahed Zeinab 
Published: August 2024

AbstractMaterials with engineered nano-scale surface topographies, such as nanopillars, nanoneedles, and nanowires, mimic natural structures like viral spike proteins, enabling them to bypass biological barriers like the plasma membrane. These properties have led to applications in nanoelectronics for intracellular sensing and drug delivery platforms, some of which are already in clinical trials. Here, we present evidence that nanotopographic materials can induce transient openings in the nuclear membranes of various cell types without penetrating the cells, breaching the nucleo-cytoplasmic barrier and allowing uncontrolled molecular exchange across the nuclear membrane. These openings, induced by nanoscale curvature, are temporary and repaired through ESCRT-mediated mechanisms. Our findings suggest a potential for nano topographic materials for direct nuclear sensing and delivery, holding promise for improving the delivery, efficiency, and safety of therapeutic agents to the nucleus.
Discovery of Z1362873773: A Novel Fascin Inhibitor from a Large Chemical Library for Colorectal Cancer
Authors: Rodríguez-Martínez Alejandro , Giraldo-Ruiz Lucía , Ramos Maria C. , Luque Irene , Ribeiro Diogo , Postigo-Corrales Fátima , Alburquerque-González Begoña , Montoro-García Silvia , Arroyo-Rodríguez Ana Belén , Conesa-Zamora Pablo , Hurtado Ana María , Luengo-Gil Ginés , Pérez-Sánchez Horacio 
Published: August 2024

AbstractMetastasis is one of the leading causes of cancer-related death worldwide. Fascin is involved in this process by bundling actin filaments and producing protrusions in cancer cells, which facilitate their migration. It has been shown that the overexpression of this protein is related to the appearance of different types of cancer, such as colorectal cancer. In this study, we conducted an in silico screening against the enamine library, a compound library with a broad chemical space (≈1.4M compounds), followed by further validation with physicochemical assays and cellular migration and cytotoxicity tests, thereby obtaining a molecule with considerable fascin inhibitory and migration-arresting capacity similar to other inhibitors already known in the literature.Graphical Abstract
Genetic differentiation and adaptive evolution of buff-tailed bumblebees in Asia
Authors: Su Long , Ding Lele , Williams Paul H. , Liu Yan , Wang Ruijuan , Dai Xiaoyan , Zhao Shan , Fu Haolin , Zhao Xiaomeng , Wang Quangui , Li Yancan , Sang Huiling , Waterhouse Robert M. , Zhai Yifan , Sun Cheng 
Published: August 2024

AbstractBumblebees are ecologically and economically important pollinating insects, so their declines resulting from environmental changes have received intensive attention. Understanding how environmental factors shape the genetic differentiation of natural populations and identifying the genetic basis of local adaptation will provide insights into how species may cope with environmental changes. The buff-tailed bumblebee (Bombus terrestris) has a wide natural distribution range and has been successfully domesticated to produce commercial colonies for greenhouse pollination. Previous population genetics studies onB. terrestrishave mainly focused on populations in Europe; however, populations in Asia, representing the eastern side of its natural distribution, have been less thoroughly sampled. To fill this gap, we collected wildB. terrestrissamples from Asia, as well as wildB. terrestrisfrom Europe and samples from domesticated colonies. We conducted whole-genome resequencing for 77 collectedB. terrestrisworkers and performed population genomics analysis. Our results revealed significant genetic differentiation (FST= 0.076) between buff-tailed bumblebees in Europe and Asia, along with notable morphological and physiological differences. Consequently,B. terrestrisin Asia represents a distinct new genetic resource. Demographic analysis suggested that the population size of buff-tailed bumblebees had increased during historic cold periods, confirming their cold-adapted characteristics. Selective sweep analysis identified 331 genes under selection in the genomes of AsianB. terrestris, likely involved in their adaptation to high ultraviolet radiation, low temperature, and low precipitation in their habitats. Our research provides insights into the population genetic structure and genetic basis of local adaptation in the buff-tailed bumblebee, which will be useful for its conservation and management.
Microbial reaction rate estimation using proteins and proteomes
Authors: McCain J. Scott P. , Britten Gregory L. , Hackett Sean R. , Follows Michael J. , Li Gene-Wei 
Published: August 2024

AbstractMicrobes transform their environments using diverse enzymatic reactions. However, it remains challenging to measure microbial reaction rates in natural environments. Despite advances in global quantification of enzyme abundances, the individual relationships between enzyme abundances and their reaction rates have not been systematically examined. Using matched proteomic and reaction rate data from microbial cultures, we show that enzyme abundance is often insufficient to predict its corresponding reaction rate. However, we discovered that global proteomic measurements can be used to make accurate rate predictions of individual reaction rates (medianR2= 0.78). Accurate rate predictions required only a small number of proteins and they did not need explicit prior mechanistic knowledge or environmental context. These results indicate that proteomes are encoders of cellular reaction rates, potentially enabling proteomic measurementsin situto estimate the rates of microbially mediated reactions in natural systems.SignificanceOne of the most basic phenotypes of a microbe is its set of associated reaction rates, but quantifying these ratesin situremains extremely challenging, especially in natural systems. We used molecular data and statistical models to estimate microbial rates in steady state cultures. We found that many reaction rates are highly predictable using proteomic data, though single proteins are typically not informative for their associated reaction rates. This result suggests that gene expression data from complex microbial communities could be used to estimatein situreaction rates, providing new clues into the lives and environmental function of microbes.
Network models incorporating chloride dynamics predict optimal strategies for terminating status epilepticus
Authors: Currin Christopher B. , Burman Richard J. , Fedele Tommaso , Ramantani Georgia , Rosch Richard E. , Sprekeler Henning , Raimondo Joseph V. 
Published: August 2024

ABSTRACTSeizures that continue for beyond five minutes are classified as status epilepticus (SE) and constitute a medical emergency. Benzodiazepines, the current first-line treatment, attempt to terminate SE by increasing the conductance of chloride-permeable type-A GABA receptors (GABAARs). Despite their widespread use, benzodiazepines are ineffective in over a third of cases. Previous research in animal models has demonstrated that changes in intraneuronal chloride homeostasis and GABAAR physiology may underlie the development of benzodiazepine resistance in SE. However, there remains a need to understand the effect of these changes at a network level to improve translation into the clinical domain. Therefore, informed by data from human EEG recordings of SE and experimental brain slice recordings, we used a large spiking neural network model that incorporates chloride dynamics to investigate and address the phenomenon of benzodiazepine resistance in SE. We found that the GABAAR reversal potential (EGABA) sets SE-like bursting and determines the response to GABAAR conductance modulation, with benzodiazepines being anti-seizure at low EGABAand ineffective or pro-seizure at high EGABA. The SE-like activity and EGABAdepended on a non-linear relationship between the strength of Cl-extrusion and GABAAR conductance, but not on the initial EGABAof neurons. Independently controlling Cl-extrusion in the pyramidal and interneuronal cell populations revealed the critical role of pyramidal cell Cl-extrusion in determining the severity of SE activity and the response to simulated benzodiazepine application. Finally, we demonstrate the model’s utility for considering improved therapeutic approaches for terminating SE in the clinic.