bluuebunny/crossref_metadata_2025_split huggingface dataset:
Time-Mean Flow as the Prevailing Contribution to the Poleward Heat Flux across the Southern Flank of the Antarctic Circumpolar Current: A Case Study in the Fawn Trough, Kerguelen Plateau
Authors: Sekma H. , Park Y.-H. , Vivier F. 
Published: November 2012

Abstract
The major mechanisms of the oceanic poleward heat flux in the Southern Ocean are still in debate. The long-standing belief stipulates that the poleward heat flux across the Antarctic Circumpolar Current (ACC) is mainly due to mesoscale transient eddies and the cross-stream heat flux by time-mean flow is insignificant. This belief has recently been challenged by several numerical modeling studies, which stress the importance of mean flow for the meridional heat flux in the Southern Ocean. Here, this study analyzes moored current meter data obtained recently in the Fawn Trough, Kerguelen Plateau, to estimate the cross-stream heat flux caused by the time-mean flow and transient eddies. It is shown that the poleward eddy heat flux in this southern part of the ACC is negligible, while that from the mean flow is overwhelming by two orders of magnitude. This is due to the unusual anticlockwise turning of currents with decreasing depth, which is associated with significant bottom upwelling engendered by strong bottom currents flowing over the sloping topography of the trough. The circumpolar implications of these local observations are discussed in terms of the depth-integrated linear vorticity budget, which suggests that the six topographic features along the southern flank of the ACC equivalent to the Fawn Trough case would yield sufficient poleward heat flux to balance the oceanic heat loss in the subpolar region. As eddy activity on the southern flank of the ACC is too weak to transport sufficient heat poleward, the nonequivalent barotropic structure of the mean flow in several topographically constricted passages should accomplish the required task.
Associations Between Timing in the Baseball Pitch and Shoulder Kinetics, Elbow Kinetics, and Ball Speed
Authors: Urbin M.A. , Fleisig Glenn S. , Abebe Asheber , Andrews James R. 
Published: November 2012

Background: A baseball pitcher’s ability to maximize ball speed while avoiding shoulder and elbow injuries is an important determinant of a successful career. Pitching injuries are attributed to microtrauma brought about by the repetitive stress of high-magnitude shoulder and elbow kinetics. Hypothesis: Over a number of pitches, variations in timing peak angular velocities of trunk segment rotations will be significantly associated with ball speed and upper extremity kinetic parameters. Study Design: Descriptive laboratory study. Methods: Kinematic and kinetic data were derived from 9 to 15 fastball pitches performed by 16 active, healthy collegiate (n = 8) and professional (n = 8) pitchers via 3-dimensional motion capture (240 Hz). Each pitch was decomposed into 4 phases corresponding to the time between peak angular velocities of sequential body segment rotations. Four mixed models were used to evaluate which phases varied significantly in relation to ball speed, peak shoulder proximal force, peak shoulder internal rotation torque, and peak elbow varus torque. Mixed-model parameter coefficient estimates were used to quantify the influence of these variations in timing on ball speed and upper extremity kinetics. Results: All 4 mixed models were significant ( P < .05). The time from stride-foot contact to peak pelvis angular velocity varied significantly in relation to all upper extremity kinetic parameters and ball speed. Increased time in this phase correlated with decreases in all parameters. Decreased ball speed also correlated with increased time between peak upper torso and elbow extension angular velocities. Decreased shoulder proximal force also correlated with increased time between peak pelvis and upper torso angular velocities. Conclusion: There are specific phases that vary in relation to ball speed and upper extremity kinetic parameters, reinforcing the importance of effectively and consistently timing segmental interactions. For the specific interactions that varied significantly, increased phase times were associated with decreased kinetics and ball speed. Clinical Relevance: Although increased time within specific phases correlates with decreases in the magnitude of upper extremity kinetics linked to overuse injuries, it also correlates with decreased ball speed. Based on these findings, it may appear that minimizing the risk of injury (ie, decreased kinetics) and maximizing performance quality (ie, increased ball speed) are incompatible with one another. However, there may be an optimal balance in timing that is effective for satisfying both outcomes.
Chlamydia trachomatis genovar distribution in clinical urogenital specimens from Tunisian patients: high prevalence of C. trachomatisgenovar E and mixed infections
Authors: Gharsallah Houda , Frikha-Gargouri Olfa , Sellami Hanen , Besbes Fatma , Znazen Abir , Hammami Adnene 
Published: November 2012

AbstractBackgroundThis epidemiological study was carried out in Sfax (south of Tunisia) and focused on genitalChlamydia trachomatis(C. trachomatis) genovar distribution.MethodsOne hundred and thirty seven genital samples from 4067 patients (4.2%) attending the Habib Bourguiba University hospital of Sfax over 12 years (from 2000 to 2011) were found to beC. trachomatisPCR positive by the Cobas Amplicor system. These samples were genotyped by an in house reverse hybridization method.ResultsOne hundred and eight (78.8%) samples contained only one genovar and 29 (21.2%) samples contained two or three genovars. Genovar E was the most prevalent (70.8%) single genovar and it was detected in 90.6% of all the cases. Genovars J, C and L1-L3 were not detected in our samples whereas ocular genovars A and B were in 5 cases. All the five cases were mixed infections. Men had more mixed infections than women (p=0.02) and were more frequently infected by genovars F and K (p<0.05). No associations between current infection, infertility and the genovar distribution were observed. Patients coinfected withNeisseria gonorrhoeaewere also significantly more frequently infected with mixed genovars (p=0.04).ConclusionsIn conclusion, we have reported a high prevalence of genovar E and of mixed infections in our study population. Such data could have implications for the control and vaccine development ofC. trachomatisin Tunisia.
Thymic stromal lymphopoietin gene promoter polymorphisms and expression levels in Graves’ disease and Graves’ ophthalmopathy
Authors: Tsai Kun-Hsi , Tsai Fuu-Jen , Lin Hui-Ju , Lin Hung-Jung , Liu Yu-Huei , Liao Wen-Ling , Wan Lei 
Published: November 2012

Abstract

Background
Graves disease (GD) is an organ-specific autoimmune disease characterized by hyperthyroidism, diffuse goiter, autoantibodies against thyroid-specific antigens, and dermopathy. Studies of GD have demonstrated the importance of the Th2 and Th17 immune responses in mediating disease progression. In the present study, we investigated the role of a Th2 cytokine, thymic stromal lymphopoietin (TSLP), in GD and Th17 differentiation.

Methods
In this study, we genotyped 470 patients with GD at 3 single nucleotide polymorphisms (SNPs) in TSLP. In addition, the serum concentrations of TSLP were determined in 432 patients and 272 controls. Ten patients and controls each were further screened using in vitro Th17 differentiation assays. The SNPs were genotyped using ABI TaqMan® SNP genotyping assays. For the Th17 differentiation assays, peripheral blood mononuclear cells (PBMCs) isolated from the patients and controls were placed into Th17 differentiation media, and interleukin 17 expression levels were determined.

Results
Haplotype analysis indicated that patients with the Ht3 (TCC) haplotype have a 3.28-fold higher risk of developing GD (p = 0.007), whereas those with the Ht5 (TCG) haplotype had a 0.03-fold, reduced risk of developing GD (p = 1 × 10−14). SNP rs3806933 (p = 0.007) was associated with female Graves ophthalmopathy (GO). TSLP expression levels were higher in GD patients than in control subjects, and TLSP was also shown to promote the differentiation of Th17 cells in GD patients.

Conclusions
These results suggest that polymorphisms in TSLP may be used as genetic markers for the diagnosis and prognosis of GD. Furthermore, TLSP may be a target for treating GD.
Effects of Sn doping on the morphology and properties of Fe-doped In2O3 epitaxial films
Authors: Zhou Tie , Wei Lin , Xie Yanru , Li Qinghao , Hu Guoxiang , Chen Yanxue , Yan Shishen , Liu Guolei , Mei Liangmo , Jiao Jun 
Published: November 2012

Abstract
(Sn, Fe)-codoped In2O3 epitaxial films were deposited on (111)-oriented Y-stabilized ZrO2 substrates by pulsed laser deposition with constant Fe concentration and different Sn concentrations. The influence of Sn concentration on the crystal structure and properties of Fe-doped In2O3 ferromagnetic semiconductor films has been investigated systematically. Experimental results indicate that Sn doping can effectively reduce the surface roughness and suppresses breakup of the films into separated islands. At the same time, the optical band gap increases and the electrical properties improve correspondingly. However, although the carrier density increases dramatically with the Sn doping, no obvious change of the ferromagnetism is observed. This is explained by a modified bounded magnetic polaron model.
Zeroing in on AIDS and global health Post-2015
Authors: Buse Kent , Blackshaw Ruth , Ndayisaba Marie-Goretti Harakeye 
Published: November 2012

Abstract
December 1st marks World AIDS Day with the theme ‘Getting to zero’. Three years ago, UNAIDS articulated what was then considered to be an ambitious vision, the aspiration for zero new HIV infections and zero-AIDS related deaths underpinned by zero discrimination. As we imagine the Post-2015 development agenda, we can and should reconceptualise this vision as a set of concrete goals.
This Viewpoint argues that today’s rapidly changing world, including its shifting geo-political and economic landscape, requires policy responses that are context-sensitive. We highlight the Shared Responsibility-Global Solidarity agenda, as pioneered by the African Union in its recent Roadmap on AIDS, tuberculosis, and malaria, to illustrate ways in which global health can be re-thought to tackle twenty-first century challenges. In light of the emerging debate on what a Post-2015 development agenda and accountability framework should look like, we argue that the AIDS response offers lessons as a pathfinder which can pave the way for global health responses in which the most marginalised are at the centre of the debate, human rights are protected under the rule of law, strong accountability is in place for results for people, and community and participatory processes are the norm. These hard-learned and -won principles of the AIDS response are critical if we are to realize a world in which there is zero inequality and health justice for all.
Distinct genetic alterations occur in ovarian tumor cells selected for combined resistance to carboplatin and docetaxel
Authors: Armstrong Stephen R , Narendrula Rashmi , Guo Baoqing , Parissenti Amadeo M , McCallum Katherine L , Cull Stephanie , Lannér Carita 
Published: November 2012

AbstractBackgroundCurrent protocols for the treatment of ovarian cancer include combination chemotherapy with a platinating agent and a taxane. However, many patients experience relapse of their cancer and the development of drug resistance is not uncommon, making successful second line therapy difficult to achieve. The objective of this study was to develop and characterize a cell line resistant to both carboplatin and docetaxel (dual drug resistant ovarian cell line) and to compare this cell line to cells resistant to either carboplatin or docetaxel.MethodsThe A2780 epithelial endometrioid ovarian cancer cell line was used to select for isogenic carboplatin, docetaxel and dual drug resistant cell lines. A selection method of gradually increasing drug doses was implemented to avoid clonal selection. Resistance was confirmed using a clonogenic assay. Changes in gene expression associated with the development of drug resistance were determined by microarray analysis. Changes in the expression of selected genes were validated by Quantitative Real-Time Polymerase Chain Reaction (QPCR) and immunoblotting.ResultsThree isogenic cell lines were developed and resistance to each drug or the combination of drugs was confirmed. Development of resistance was accompanied by a reduced growth rate. The microarray and QPCR analyses showed that unique changes in gene expression occurred in the dual drug resistant cell line and that genes known to be involved in resistance could be identified in all cell lines.ConclusionsOvarian tumor cells can acquire resistance to both carboplatin and docetaxel when selected in the presence of both agents. Distinct changes in gene expression occur in the dual resistant cell line indicating that dual resistance is not a simple combination of the changes observed in cell lines exhibiting single agent resistance.
Membrane-displayed somatostatin activates somatostatin receptor subtype-2 heterologously produced in Saccharomyces cerevisiae
Authors: Hara Keisuke , Shigemori Tomohiro , Kuroda Kouichi , Ueda Mitsuyoshi 
Published: November 2012

Abstract
The G-protein-coupled receptor (GPCR) superfamily, which includes somatostatin receptors (SSTRs), is one of the most important drug targets in the pharmaceutical industry. The yeast Saccharomyces cerevisiae is an attractive host for the ligand screening of human GPCRs. Here, we demonstrate the utility of the technology that was developed for displaying peptide ligands on yeast plasma membrane, termed “PepDisplay”, which triggers signal transduction upon GPCR activation. A yeast strain that heterologously produced human somatostatin receptor subtype-2 (SSTR2) and chimeric Gα protein was constructed along with membrane-displayed somatostatin; somatostatin was displayed on the yeast plasma membrane by linking it to the anchoring domain of the glycosylphosphatidylinositol anchored plasma membrane protein Yps1p. We demonstrate that the somatostatin displayed on the plasma membrane successfully activated human SSTR2 in S. cerevisiae. The methodology presented here provides a new platform for identifying novel peptide ligands for both liganded and orphan mammalian GPCRs.
Each to their own: skeletal muscles of different function use different biochemical strategies during aestivation at high temperature
Authors: Young Karen M. , Cramp Rebecca L. , Franklin Craig 
Published: November 2012

SummaryPreservation of muscle morphology depends on a continuing regulatory balance between molecules that protect, and molecules that damage, muscle structural integrity. Excessive disruption of the biochemical balance that favours reactive oxygen species (ROS) in disused muscles may lead to oxidative stress; which in turn is associated with increased atrophic or apoptotic signalling and/or oxidative damage to the muscle and thus muscle disuse atrophy. Increases in rate of oxygen consumption likely increase the overall generation of ROS in vivo. Temperature-induced increases in muscle oxygen consumption rate occur in some muscles of ectotherms undergoing prolonged muscular disuse during aestivation. In the green-striped burrowing frog, Cyclorana alboguttata, both large jumping muscles and small non-jumping muscles undergo atrophy seemingly commensurate with their rate of oxygen consumption during aestivation. However, since the extent of atrophy in these muscles is not enhanced at higher temperatures despite a temperature sensitive rate of oxygen consumption in the jumping muscle, we proposed that muscles are protected by biochemical means that when mobilised at higher temperatures inhibit atrophy. We proposed the biochemical response to temperature would be muscle-specific. We examined the effect of temperature on the antioxidant and heat shock protein systems and evidence of oxidative damage to lipids and proteins in two functionally different skeletal muscles, gastrocnemius (jumping muscle) and iliofibularis (non-jumping muscle), by aestivating frogs at 24 and 30oC for six months. We assayed small molecule antioxidant capacity, mitochondrial and cytosolic SOD and Hsp70 to show that protective mechanisms in disused muscles are differentially regulated both with respect to temperature and aestivation. High aestivation temperature results in an antioxidant response in the metabolically temperature-sensitive jumping muscle. We assayed lipid peroxidation and protein oxidation to show that oxidative damage is apparent during aestivation and its pattern is muscle-specific, but unaffected by temperature. Consideration is given to how the complex responses of muscle biochemistry inform of the different strategies muscles may use in regulating their oxidative environment during extended disuse and disuse at high temperature.
Contributions of phenotypic plasticity to differences in thermogenic performance between highland and lowland deer mice
Authors: Cheviron Zachary A. , Bachman Gwendolyn C. , Storz Jay F. 
Published: November 2012

SummarySmall mammals face especially severe thermoregulatory challenges at high-altitude because the reduced O2 availability constrains the capacity for aerobic thermogenesis. Adaptive enhancement of thermogenic performance under hypoxic conditions may be achieved via physiological adjustments that occur within the lifetime of individuals (phenotypic plasticity) and/or genetically based changes that occur across generations, but their relative contributions to performance differences between highland and lowland natives is unclear. Here, we examined potentially evolved differences in thermogenic performance between populations of deer mice (Peromyscus maniculatus) that are native to different altitudes. The purpose of the study was to assess the contribution of phenotypic plasticity to population differences in thermogenic performance under hypoxia. We used a common-garden deacclimation experiment to demonstrate that highland deer mice have enhanced thermogenic capacities under hypoxia, and that performance differences between highland and lowland mice persist when individuals are born and reared under common-garden conditions, suggesting that differences in thermogenic capacity have a genetic basis. Conversely, population differences in thermogenic endurance appear to be entirely attributable to physiological plasticity during adulthood. These combined results reveal distinct sources of phenotypic plasticity for different aspects of thermogenic performance, and suggest that thermogenic capacity and endurance may have different mechanistic underpinnings.
Condition-dependent auditory processing in the round goby (Neogobius melanostomus): links to sex, reproductive condition, and female estrogen levels.
Authors: Zeyl Jeffrey N. , Love Oliver P. , Higgs Dennis M. 
Published: November 2012

SummaryNeural responses to sensory stimuli often differ between sexes, vary seasonally, and can be regulated by endocrine activity, but the ecological and physiological mechanisms driving such patterns are not well understood. The current study examined how auditory function in the round goby (Neogobius melanostomus), a vocal teleost, co-varied with sex, reproductive condition, and female plasma 17β-estradiol. Auditory evoked potentials were collected in response to tone pips (100-600 Hz) and a natural round goby pulse vocalization. Additionally, saccule hair cell densities were compared across reproductive groups. Auditory threshold was evaluated in terms of pressure and particle acceleration, and response amplitude and onset latency were measured at 10 dB above threshold. Relative to males, females displayed lower auditory thresholds in response to the natural vocalization and to tones at 300–600 Hz, and had a higher density of saccule hair cells. 17β-estradiol level was positively associated with amplitude and latency for the pulse stimulus and with both threshold and amplitude for tones at 100-200 Hz in females. Relative to non-reproductive males, reproductive males exhibited longer response latencies at 100-200 Hz. The results demonstrate sexual dimorphism in auditory function in a teleost fish as well as intra-sexual variation, partially based on hormone levels. The current research further identifies links between auditory function and reproductive behaviors in fishes and provides a finer-scaled analysis of how this behavior is reflected at the level of the sensory systems facilitating signal reception.
Differences in contractile behaviour between the soleus and medial gastrocnemius muscles during human walking
Authors: Cronin Neil J. , Peltonen Jussi T. , Finni Taija , Avela Janne 
Published: November 2012

Summary
The functional roles of individual lower limb muscles during human walking may differ depending on walking speed or duration. In this study, eleven volunteers walked on a treadmill for 60 minutes at speeds corresponding to both optimal and 20% above optimal energetic cost of transport whilst oxygen consumption and medial gastrocnemius (MG) and soleus fascicle lengths were measured. Although energetic cost of transport was ~12% higher at the faster speed, it remained constant over 60 minutes at both speeds, suggesting that humans can walk for prolonged periods at a range of speeds without compromising energetic efficiency. The fascicles of both muscles exhibited rather 'isometric' behaviour during the early to mid stance phase of walking, which appears to be independent of walking speed or movement efficiency. However, several functional differences were observed between muscles: MG exhibited time- and speed-dependent decreases in operating length, and shortened faster during the pushoff phase at the faster walking speed. Conversely, soleus exhibited consistent contractile behaviour regardless of walking speed or duration, and always shortened slower than MG during pushoff. Soleus appears to play a more important functional role than MG during walking. This may be especially true when walking for prolonged periods or at speeds above the most energetically efficient, where the force potential and thus the functional importance of MG appears to decline.
Protein kinase A dependent and independent activation of the V-ATPase in Malpighian tubules ofAedes aegypti.
Authors: Tiburcy Felix , Beyenbach Klaus W. , Wieczorek Helmut 
Published: November 2012

SummaryTransepithelial ion transport in insect Malpighian tubules is energized by an apical V-ATPase. In hematophagous insects, a blood meal during which the animal ingests huge amounts of salt and water stimulates transepithelial transport processes linked to V-ATPase activation, but how this is accomplished is still unclear. Here we report that membrane-permeant derivatives of cAMP increase the bafilomycin-sensitive ATPase activity in Malpighian tubules of Aedes aegypti twofold and activate ATP-dependent transport processes. In parallel, membraneassociation of the V1 subunits C and D increases, consistent with the assembly of the holoenzyme. The protein kinase A inhibitor H-89 abolishes all cAMP-induced effects, consistent with PKA being involved in V-ATPase activation. Metabolic inhibition induced by KCN, azide and 2,4-dinitrophenol, respectively, also induces assembly of functional V-ATPases at the membrane without protein kinase A involvement, indicating a phosphorylation independent activation mechanism.