bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between biomarkers of endothelial injury and hypocoagulability in patients with severe sepsis: a prospective study
Authors: Ostrowski Sisse Rye , Haase Nicolai , Müller Rasmus Beier , Møller Morten Hylander , Pott Frank Christian , Perner Anders , Johansson Pär Ingemar 
Published: April 2015

Abstract
Introduction
Patients with severe sepsis often present with concurrent coagulopathy, microcirculatory failure and evidence of vascular endothelial activation and damage. Given the critical role of the endothelium in balancing hemostasis, we investigated single-point associations between whole blood coagulopathy by thrombelastography (TEG) and plasma/serum markers of endothelial activation and damage in patients with severe sepsis.

Methods
A post-hoc multicenter prospective observational study in a subgroup of 184 patients from the Scandinavian Starch for Severe Sepsis/Septic Shock (6S) Trial. Study patients were admitted to two Danish intensive care units. Inclusion criteria were severe sepsis, pre-intervention whole blood TEG measurement and a plasma/serum research sample available from baseline (pre-intervention) for analysis of endothelial-derived biomarkers. Endothelial-derived biomarkers were measured in plasma/serum by enzyme-linked immunosorbent assay (syndecan-1, thrombomodulin, protein C (PC), tissue-type plasminogen activator and plasminogen activator inhibitor-1). Pre-intervention TEG, functional fibrinogen (FF) and laboratory and clinical data, including mortality, were retrieved from the trial database.

Results
Most patients presented with septic shock (86%) and pulmonary (60%) or abdominal (30%) focus of infection. The median (IQR) age was 67 years (59 to 75), and 55% were males. The median SOFA and SAPS II scores were 8 (6 to 10) and 56 (41 to 68), respectively, with 7-, 28- and 90-day mortality rates being 21%, 39% and 53%, respectively. Pre-intervention (before treatment with different fluids), TEG reaction (R)-time, angle and maximum amplitude (MA) and FF MA all correlated with syndecan-1, thrombomodulin and PC levels. By multivariate linear regression analyses, higher syndecan-1 and lower PC were independently associated with TEG and FF hypocoagulability at the same time-point: 100 ng/ml higher syndecan-1 predicted 0.64 minutes higher R-time (SE 0.25), 1.78 mm lower TEG MA (SE 0.87) and 0.84 mm lower FF MA (SE 0.42; all P <0.05), and 10% lower protein C predicted 1.24 mm lower TEG MA (SE 0.31).

Conclusions
In our cohort of patients with severe sepsis, higher circulating levels of biomarkers of mainly endothelial damage were independently associated with hypocoagulability assessed by TEG and FF. Endothelial damage is intimately linked to coagulopathy in severe sepsis.

Trial registration
Clinicaltrials.gov number: NCT00962156. Registered 13 July 2009.
Crustal-scale structure and deformation of Lu-Zong ore district: Joint interpretation from integrated geophysical data
Authors: Lü Qingtian , Liu Zhendong , Yan Jiayong , Tang Jingtian , Wu Mingan , Xiao Xiao 
Published: April 2015

We have studied five intersecting integrated geophysical profiles for the 3D crustal structure of the Lu (Lujiang)-Zong (Zongyang) ore district to obtain a better understanding of the metallogenesis and provide in-depth information for deep mineral targeting. The profiles, totaling more than 300 km, have reflection seismics and magnetotelluric sounding. Regional gravity surveys were also integrated into this study. New discoveries were obtained regarding the upper crustal structure and deformation based on the integrated analysis of these data. The Lu-Zong ore district consists of four major crustal blocks. They are the Shaxi uplift, Qianshan-Kongcheng Depression (QKD) in the west, Lu-Zong volcanic basin, and the Along-River uplift in the east. The north–south crustal elements show the northward “step-type” uplift, juxtaposed by two steplike faults, the west–northwest/east–southeast-trending Tangjiayuan-Zhuanqiao Fault and the Lujiang-Huangguzha-Tongling Detachment (LHTD) Fault. The Lu-Zong volcanic basin presents a nonsymmetrical shape with four inward-dipping boundary faults. The northern and eastern boundary faults (BF2 and LHTD) are deep faults, which control the development and evolution of the Lu-Zong volcanic basin. There are three west–northwest/east–southeast-trending faults and six northeast–southwest-trending faults cutting over the ore district. From north to south, they are the LHTD Fault, Tangjiayuan-Zhuanqiao Fault, and Yijing-Taojiaxiang Fault (BF3); from west to east, the six faults are the Tan-Lu, Chuhe, Luohe-Quekou, Zongyang-Huangtun, Taojiawan-Shijiawan, and Changjiang Thrust Fault (CTF). The formation and evolution of the ore-district are mainly affected by the Yanshanian intracontinental orogeny, and they experience the Middle to Late Jurassic compression and subsequent Cretaceous extension, possibly due to the paleo-Pacific northwest-trending subduction. Our studies determined that the CTF is a thrust system in nature and the LHTD is a southwest-dipping detachment. Two Jurassic basins were found, surrounding the northeast and southeast of Lu-Zong volcanics, which may be due to the product of postcollision extension of Indosinian orogeny during the middle and early Late Triassic.
Synthesis of New Electron Donor ClMe3-TTP: Structures and Properties of (ClMe3-TTP)3X (X = PF6 and AsF6)
Authors: Shirahata Takashi , Kohno Shuhei , Furuta Keisuke , Oka Yusuke , Misaki Yohji 
Published: April 2015

Abstract
In this study, we synthesized 2-(4-chloro-5-methyl-1,3-dithiol-2-ylidene)-5-(4,5-dimethyl-1,3-dithiol-2-ylidene)-1,3,4,6-tetrathiapentalene (ClMe3-TTP). Results show that the cation radical salts of ClMe3-TTP with PF6− and AsF6− anions show high conductivities (101 S cm−1) at room temperature and semiconducting behavior with low activation energy (24–27 meV). A crystal structure analysis of the PF6− and AsF6− salts reveals a 3:1 donor–anion ratio of these salts. The ClMe3-TTP molecules form one-dimensional columns along the a axis, and tape-structures, connected by the side-by-side S···S interactions, also form along the a axis. The calculated intracolumn and intratape overlap integrals are relatively large; however, the overlap integrals between the one-dimensional columns and the tapes are approximately 1/2–1/5 the value of the intracolumn and intratape overlap integrals. The band calculations suggest that they have one-dimensional electronic structures, indicating that they would comprise a Mott insulator.
Models and Insights for Hospital Inpatient Operations: Time-Dependent ED Boarding Time
Authors: Shi Pengyi , Chou Mabel C. , Dai J. G. , Ding Ding , Sim Joe 
Published: April 2015

One key factor contributing to emergency department (ED) overcrowding is prolonged waiting time for admission to inpatient wards, also known as ED boarding time. To gain insights into reducing this waiting time, we study operations in the inpatient wards and their interface with the ED. We focus on understanding the effect of inpatient discharge policies and other operational policies on the time-of-day waiting time performance, such as the fraction of patients waiting longer than six hours in the ED before being admitted. Based on an empirical study at a Singaporean hospital, we propose a novel stochastic processing network with the following characteristics to model inpatient operations: (1) A patient’s service time in the inpatient wards depends on that patient’s admission and discharge times and length of stay. The service times capture a two-time-scale phenomenon and are not independent and identically distributed. (2) Pre- and post-allocation delays model the extra amount of waiting caused by secondary bottlenecks other than bed unavailability, such as nurse shortage. (3) Patients waiting for a bed can overflow to a nonprimary ward when the waiting time reaches a threshold, where the threshold is time dependent. We show, via simulation studies, that our model is able to capture the inpatient flow dynamics at hourly resolution and can evaluate the impact of operational policies on both the daily and time-of-day waiting time performance. In particular, our model predicts that implementing a hypothetical policy can eliminate excessive waiting for those patients who request beds in mornings. This policy incorporates the following components: a discharge distribution with the first discharge peak between 8 a.m. and 9 a.m. and 26% of patients discharging before noon, and constant-mean allocation delays throughout the day. The insights gained from our model can help hospital managers to choose among different policies to implement depending on the choice of objective, such as to reduce the peak waiting in the morning or to reduce daily waiting time statistics. This paper was accepted by Assaf Zeevi, stochastic models and simulation.
Can school nurses identify mental health needs early and provide effective advice and support?
Authors: Bartlett Heather 
Published: April 2015

Aim: This article aims to explore and analyse primary research regarding the role of the school nurse in providing care for children and young people with mental health needs. Background: Children and young people have unique mental health needs that can affect their emotional, educational and physical development. Early identification and support for mental health needs are advocated to improve health and wellbeing outcomes. There are few UK-based primary research papers that address what role the school nurse may have in providing mental health care. Methods: A database search was conducted to identify research papers that discuss ‘school nurse’, ‘mental health’ with ‘children and young people’. Eight UK-based papers were identified, on which a thematic analysis was carried out. Results: Four themes were identified as important indicators for school nurses working with children and young people with mental health needs: help seeking preferences of children and young people, training and support needs of school nurses, service provision and capacity, and wider socio-economic determinants. Conclusions: School nurses can be effective when working with children and young people with mental health needs. As specialist public health nurses their primary area of work should be on the promotion of good mental health and the early identification and support for those who have poor mental health. To ensure effective support is given school nurses require further post-graduate training and regular supervision from a mental health specialist. However, workload structures would need to be managed to allow school nurses to be more visible and accessible within schools in order to build effective and trusting relationships. Implications for practice: As the public health role of the school nurse begins to encompass mental health needs it will be increasingly important to reflect upon collaborative working practices to deliver care, which meets the need of children and young people. This may include the use of modern technology, a work schedule that allows for needs to be met year round and improved communication and discussion between health, education and social care professionals.
Parasite communities of the elongate tigerfish Hydrocynus forskahlii (Cuvier 1819) and redbelly tilapia Tilapia zillii (Gervais 1848) from Lake Turkana, Kenya: influence of host sex and size
Authors: Otachi Elick O. , Szostakowska Beata , Jirsa Franz , Fellner-Frank Christine 
Published: April 2015

AbstractFish is an important food source for an estimated 300,000 people inhabiting the shores of Lake Turkana, Kenya. Despite its large size (7560 km2) and importance, the lake remains one of the least studied in the Great Lakes Region of Africa. This study describes the parasite community of the elongate tigerfish Hydrocynus forskahlii (Cuvier, 1819) and the redbelly tilapia Tilapia zillii (Gervais, 1848). A total of 87 individuals (43 H. forskahlii and 44 T. zillii) were dissected and examined for parasites. Two taxa infected H. forskahlii, the dominant one being an anisakid nematode Contracaecum sp. (L3) (P = 83.7%, mean intensity (MI) = 46.0, abundance (A) = 38.5). Twelve parasite taxa were recovered from T. zillii, with metacestodes of the gryporhynchid cestode Amirthalingamia macracantha being dominant (P = 79.5, MI = 10.3, A = 8.2). This was the first report of a mixture of merocercoids and plerocercoids in the same host. Fish size was positively correlated with the major parasite infection levels, but, the prevalence of Contracaecum sp. in H. forskahlii was negatively correlated with size, probably reflecting ontogenetic shifts in habitats and diets with age. Fish sex was not a significant influencing factor, with the exception of Contracaecum sp. in H. forskahlii, where prevalence differed significantly between sexes (p<0.05), with the females having a higher prevalence than the males. We conclude that H. forskahlii had a poor parasitic community but that the infection levels with Contracaecum sp. were high. T. zillii had a rich parasite fauna, although, most parasites occurred at low intensities.
Larval cestodes infecting the deep-water fish, Cataetyx laticeps (Pisces: Bythitidae) from Madeira Archipelago, Atlantic Ocean
Authors: Costa Graça , Khadem Mahnaz , Dellinger Thomas , Biscoito Manuel , Melo-Moreira Egberto 
Published: April 2015

AbstractParasites of deep-water fishes are less known in comparison to the parasites of fishes living in the demersal and epipelagic ocean zones. In the present research note we report the occurrence of larval trypanorhynch cestodes in a rare deep-water fish, the deep-water brotula, Cataetyx laticeps. Based on the 28S rDNA (region D1-D3) sequence homology and the phylogenetic analysis, the larval cestodes are putatively assigned to the genus Grillotia. It is suggested that the definitive host of this trypanorhynch is a batoid.
Antiparasitic DNA vaccines in 21st century
Authors: Wedrychowicz Halina 
Published: April 2015

AbstractDemands for effective vaccines to control parasitic diseases of humans and livestock have been recently exacerbated by the development of resistance of most pathogenic parasites to anti-parasitic drugs. Novel genomic and proteomic technologies have provided opportunities for the discovery and improvement of DNA vaccines which are relatively easy as well as cheap to fabricate and stable at room temperatures. However, their main limitation is rather poor immunogenicity, which makes it necessary to couple the antigens with adjuvant molecules. This paper review recent advances in the development of DNA vaccines to some pathogenic protozoa and helminths. Numerous studies were conducted over the past 14 years of 21
Ultrastructural characterization of Acarispora falculifera n.gen., n.sp., a new microsporidium (Opisthokonta: Chytridiopsida) from the feather mite Falculifer rostratus (Astigmata: Pterolichoidea)
Authors: Radek Renate , Kariton Madlen , Dabert Jacek , Alberti Gerd 
Published: April 2015

AbstractOnly about 20 species of microsporidia have been described from mites. All except one species produce typical spores with a long polar filament and a polaroplast. This paper is the first study of an atypical microsporidium infection in a feather mite (Falculifer rostratus). The infection of the pigeon feather mite is restricted to the colon epithelium where it leads to hypertrophy of the concerned cells. During sporogony, a multinucleate plasmodial aggregate is formed within a sporont (endogenous sporogony resulting in a polysporophorous vesicle). The cisterns delimiting the single sporoblasts later form the spore walls. Sporogonial stages are in direct contact to the host cell cytoplasm. Merogonial stages were not present. Spores are tiny (3.6 μm × 2.6 μm), broad oval in form and monokaryotic. The spore wall of mature spores consists of a three-layered endospore and a thin, electron-dense, wavy exospore. The polar filament is anisofilar and completely coiled in 3-4 turns. In cross-sections, it has a star-like appearance because the electron-dense core forms rounded compartments of lucent material at its surface. In superficial sections, this results in a honeycomb-like pattern. A polaroplast is missing. The polar filament arises subapically at a polar sac that lacks an internal anchoring disk. These atypical spore structures clearly classify the species from the feather mite as a member of the order Chytridiopsida. It could not be clearly affiliated to one of the known genera, so we created a new genus, Acarispora, with the species A. falculifera.
Phyllodistomum spinopapillatum sp. nov. (Digenea: Gorgoderidae), from the Oaxaca killifish Profundulus balsanus (Osteichthyes: Profundulidae) in Mexico, with new host and locality records of P. inecoli: Morphology, ultrastructure and molecular evidence
Authors: León Gerardo Pérez-Ponce de , Pinacho-Pinacho Carlos D. , Mendoza-Garfias Berenit , García-Varela Martin 
Published: April 2015

AbstractPhyllodistomum spinopapillatum sp. nov. is described from the urinary bladder of the Oaxaca killifish, Profundulus balsanus Ahl (Profundulidae) in Rio Pueblo Viejo and Rio Santa Cruz, Oaxaca, southwestern Mexico. The new species is described based on evidence gathered from morphology, scanning electron microscopy (SEM), and partial sequences of the 28S rRNA gene. Diagnostic characters of the new species of Phyllodistomum Braun 1899 are the presence of spines on the entire body surface and having a ventral sucker almost half the size of oral sucker. The new species possess a large number of dome-like papillae on the body surface with scattered distribution along the hindbody, and these papillae are characteristically spinulated. Phyllodistomum spinopapillatum sp. nov. most closely resembles P. inecoli Razo-Mendivil, Perez-Ponce de Leon and Rubio- Godoy, 2013, a parasite of the twospot livebearer Pseudoxiphophorus bimaculatus (Heckel) from Veracruz, in the Atlantic slope of Mexico. In addition to the new species, specimens of P. inecoli were also found parasitising the urinary bladder of cyprinodontiforms such as the Mexican molly Poecilia sphenops Valencienes in a pond at Santa Maria Coyotepec, and in Profundulus sp. in Rio Templo, both in Oaxaca, and in the Porthole livebearer Poeciliopsis gracilis (Heckel) in Rio San Juan, as well as in Profundulus punctatus (Gunter) from Rio Nueva Francia, both in Chiapas. The distribution and host range of P. inecoli is extended to freshwaters of the Pacific slope of Mexico, and to other cyprynodontiforms.
Diphyllidean cestodes from the bigeye houndshark Iago omanensis (Norman) (Carcharhiniformes: Triakidae) in the Gulf of Oman, with the description of Coronocestus ehsanentezarii sp. nov. (Echinobothriidae)
Authors: Haseli Mohammad , Azad Samira 
Published: April 2015

AbstractA new species of Coronocestus Caira, Marques, Jensen, Kuchta and Ivanov, 2013 is described from Iago omanensis (Norman) from the Gulf of Oman. Coronocestus ehsanentezarii sp. nov. differs from C. musteli (Pintner, 1889) Caira, Marques, Jensen, Kuchta and Ivanov, 2013 in that its ovary is H- rather than U-shape. The new species is easily distinguished from C. diamanti (Ivanov and Lipshitz, 2006) Caira, Marques, Jensen, Kuchta and Ivanov, 2013 by the number of spines per column on the cephalic peduncle (24-36 vs 95-118). It differs from C. hormozganiense (Haseli, Malek, Palm and Ivanov, 2012) Caira, Marques, Jensen, Kuchta and Ivanov, 2013 based on a greater number of spines per column on the cephalic peduncle (24-36 vs 18-21). The new species differs from C. notoguidoi (Ivanov 1997) Caira, Marques, Jensen, Kuchta and Ivanov, 2013 in that of its scolex is craspedote rather than acraspedote. Unlike C. coronatum (Robinson 1959) Caira, Marques, Jensen, Kuchta and Ivanov, 2013, C. ehsanentezarii sp. nov. possesses 29-35 rather than 20 apical hooks in each dorso-ventral group. The new species possesses lateral hooklets with two rows (a and b designations) in each cluster. This character had been presented earlier only for Andocadoncum Abbott and Caira, 2014. Furthermore, a new locality record is presented for C. diamanti from Iago omanensis in the Gulf of Oman. Thus, I. omanensis certainly hosts two diphyllidean species simultaneously in the Gulf of Oman. The generic diagnosis of Coronocestus is also revised to include new data.
Efavirenz–eudragit E-100 nanoparticle-loaded aerosol foam for sustained release: in-vitro and ex-vivo evaluation
Authors: Vedha Hari B. N. , Narayanan N. , Dhevedaran K. 
Published: April 2015

AbstractThe control of HIV infection using antiretroviral agents, especially through targeted delivery, has attracted extensive attention in the last decade. Recently, the vaginal route of administration has become the one most recommended for HIV infections during pregnancy. Efavirenz is a non-nucleoside reverse transcriptase inhibitor anti-HIV drug and its prolonged half-life helps achieve long-term preexposure prophylaxis of HIV-1 infections; it is used as a first-line drug in the treatment using combination therapy. Nanotechnology has received attention as a major area of research into drug delivery in recent years. The aerosol foam formulation is packed under pressure and contains therapeutically active ingredients that are released as foam on the activation of an appropriate valve system. The objective of this study was to develop an aerosol foam formulation of efavirenz nanoparticles for vaginal drug delivery. The nanoparticles were prepared using the emulsion solvent evaporation method and converted into an aerosol foam formulation with the addition of 0.1 mass % of sodium lauryl sulphate as a foaming agent and pressurised with 1,1,1,2-tetrafluroethane. The nanoparticles containing the formulation and aerosol foam formulation were individually characterised by applying various tests such as particle size, zeta potential, pH, viscosity, drug content, entrapment efficiency, drug release studies and release kinetics. The compatibility of the materials was evaluated using FT-IR, polymorphic changes by TGA-DSC and also the morphological properties using SEM study. Foam density, bubble size and collapse time were evaluated in the aerosol-foam formulation; in addition, the drug content and release studies were compared with nanoparticle formulation. The results revealed that the nanoparticle-containing formulation and aerosol formulations were stable and the foam emerged as grade one with fine bubbles and became coarser over time.
Can voltammetry distinguish glycan isomers?
Authors: Trefulka Mojmír , Paleček Emil 
Published: April 2015

AbstractThe analysis of glycan isomers is an important task in glycomics. This study reveals for the first time that two oligosaccharide pentamers can be distinguished electrochemically. 3α,6α-mannopentaose (MPO) and verbascose (VBS) were modified using Os(VI)-temed and the carbohydrate adducts measured directly in the reaction mixture using cyclic voltammetric (CV) and square wave voltammetric (SWV) stripping at the hanging mercury drop electrode. Some CV and SWV responses of MPO were found to differ from those of VBS at full electrode coverage, requiring μM concentrations at short accumulation time-intervals. Similar results were obtained at partial electrode coverage at nM concentrations. These results afford an opportunity for the development of a new simple and inexpensive glycan analysis.