bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of human papillomavirus types in HER2/Neu breast cancer samples among women in Ardabil province
Authors: HAMDOLLAHZADEH SHAYAN , FEIZI IRAJ , AZGANI PEYMAN , hosseinzadeh SHAHNAZ , KARAMI CHIMAN 
Published: August 2024

Abstract

Background
The potential role of human papillomavirus (HPV) in breast carcinogenesis has been a topic of ongoing debate and investigation. While some studies have reported detecting HPV DNA in breast cancer samples, others have failed to establish a clear association. This study aimed to examine the presence of HPV in breast cancer tissue samples from patients in Ardabil, Iran, to contribute to the understanding of this potential link.
Methods
A total of 100 tissue samples, comprising 50 breast cancer samples and 50 healthy control samples, were analyzed. Fresh-frozen samples were transported and stored appropriately until processing. DNA extraction was performed using a commercial kit, and specific primers targeting the HPV L1 gene were employed for PCR amplification. Conventional PCR, agarose gel electrophoresis, and real-time PCR were utilized for HPV detection.
Results
Despite employing highly sensitive PCR techniques, including real-time PCR, no HPV DNA sequences were detected in any breast cancer tissue samples analyzed.
Conclusion
While the role of HPV in breast carcinogenesis remains an area of ongoing investigation and debate, with conflicting evidence from recent studies, the present study did not detect HPV DNA in breast cancer samples from Ardabil, Iran
The Impact of a Multidisciplinary Clinic on Diagnosis and Management of Patients with Cutaneous T-cell Lymphoma
Authors: Thomas Sierra , Taylor Mitchell , Antonson Molly , Ogah Ochanya , Wysong Ashley , Stephany Matthew 
Published: August 2024

Abstract

Multidisciplinary clinics (MDCs) have been shown to improve patient outcomes and reduce disease-specific mortality. This study aims to evaluate the impact of a MDS clinic on diagnosis, management, and clinical outcomes of patients diagnosed with CTCL. A retrospective cohort analysis of a CTCL MDS clinic was conducted between January 2018 to August 2023 at a large academic institution. Patients diagnosed with CTCL with at least one visit to the CTCL MDS clinic were included. Medical records were reviewed for patient demographics, diagnosis change, treatment management, survival outcomes, and clinicopathologic disease characteristics at 6-month intervals. A descriptive analysis was performed. 55 patients were identified (mean age 62.7; 58.1% male; 89.1% White). 80.0% of patients were diagnosed with CTCL, however, 78% were previously diagnosed with other dermatologic conditions. At initial presentation, 20% had a change in diagnosis with 12.7% changing to a benign dermatologic condition. Only 9% of patients had staging prior to initial visit, while majority (62%) received initial staging evaluation during their first visit. At the initial visit, 50.2% had an escalation of treatment, mainly of skin-directed therapy (36.4%). Over 6-month intervals, majority of patients had an escalation of treatment (21.1–51.1%). The modified severity weighted assessment tool (mSWAT) decreased over time (41.8 to 3.5), with many achieving stable disease (18.4–50%). MDS management is highly recommended for patients with CTCL. This study further highlights the important role of a CTCL MDS clinic, specifically in establishing accurate diagnoses, managing appropriate treatments, and stabilizing disease progression.
Signals of memory building-blocks: Pupil-linked brain arousal predicts event segmentation and episodic memory
Authors: Pajkossy Péter , Szőllősi Ágnes , Racsmány Mihály 
Published: August 2024

Abstract

We automatically segment the continuous stream of our experiences into events with discrete boundaries, and these events serve then as building blocks for later episodic memories. How our brain creates these discrete event representations remains elusive. Here we investigated the role of noradrenergic (NA) transmission, heavily linked to change-detection, in identifying event boundaries during listening to auditorily presented narratives. Specifically, we investigated how pupil dilation, an indirect physiological proxy of NA activity, tracks event boundaries while listening to short stories of everyday events. In a series of three studies, we could demonstrate that event boundaries trigger significant pupil dilation, and the magnitude of these dilations predicts later memory of narratives. Furthermore, we also showed that pupil dilation magnitude at each time point during the narratives scaled as a function of how frequently a set of independent raters indicated the presence of an event boundary preceding that specific story location. This set of results suggests that NA transmission influences episodic memory encoding by signaling unexpected changes in the flow of continuous experience, which then lead to the identification of event boundaries structuring long-term memory representations.
Modeling and Simulation of Genotypic TMB and Phenotypic immunogenicity Biomarkers in Cancer Immunoediting with Ising-Hamiltonian Characterization
Authors: Rojas-Domínguez Alfonso , Martínez-Vargas Irving Ulises , Alvarado Matías 
Published: August 2024

Abstract

Background and Objective: In the Tumor Micro-Environment, cancerprogression and its relationship with the Immune System (IS) is described interms of cancer immunoediting (CI) phases, each of which is characterized bydifferent types and levels of interaction between the tumor cells and elements ofthe IS, such as CD8+T cells. Said interactions are governed by genotypical (TumorMutational Burden, TMB) and phenotypical aspects pertaining to the tumor, aswell as by the strength of the IS. In this work, a computational model of CI ispresented which incorporates the TMB and the biomarker Tumor Immunogenic Phenotype (TIP) as its control parameters, and which employs the Ising-modelHamiltonian to characterize the system with respect to the CI phases.
Methods: Our model is a probabilistic multi-agent system with agents for tumorcells and for the IS. A basic version of this model was presented before; for thiswork we have produced a new implementation of our system, improved throughthe inclusion of the TMB and the TIP (i.e. whether a tumor is hot or cold). Thenew elements are integrated under a Michaelis-Menten relationship that isemployed to regulate the recruitment rate of CD8+T cells (and other IS elements),thus controlling the interactions between tumor cells and the IS.
Results: Our simulations confirm that the proposed system is capable ofconsistently generating different phases of CI, and that by varying the introducedparameters, the system is effectively controlled. However, beyond the expectedbehaviors, we also found unanticipated effects that nevertheless match well withthe literature regarding the combination of the genotypical and phenotypicalbiomarkers discussed. Moreover, the Ising-model Hamiltonian is confirmed as avaluable tool for the broad characterization of tumor-IS interaction, by reflectingclear and distinct patterns related to the phases of CI.
Conclusions: The presented model, although formed by relatively simple agents,generate emergent behaviors with which the phases of CI can be identified. Themodel is robust to the choice of its control parameters and, more importantly,provides a plausible explanation for the mechanics through which tumors withhigh TMB and high immunogenicity (i.e. hot tumors) exhibit a higher probabilityof responding to treatment by immunotherapy. Characterization via theIsing-model Hamiltonian also contributes to said explanation, by summarizing thesystem’s dynamics in a way that facilitates its analysis and future improvements.
Multifaceted Investigation into the Absence and Potential Invasion of Spotted Lanternfly (Lycorma delicatula) in Taiwan
Authors: Lin You-Sheng , Liao Jhih-Rong 
Published: August 2024

Abstract

BACKGROUND The spotted lanternfly (Lycorma delicatula), a significant agricultural and forestry pest, poses concerns due to its potential for rapid spread in favorable environments. Originating from East Asia, its distribution and ecological impacts in various regions, including Taiwan, remain subject to uncertainty. This study employed a comprehensive methodology combining field surveys, museum specimen analysis, and ecological niche modeling to assess the presence and potential invasion risks of L. delicatula in Taiwan.RESULTS Despite extensive surveys over two years and thorough reviews of museum collections, which were estimated by iNEXT3D analysis to ensure survey completeness, we found no conclusive evidence for the presence of the species in Taiwan, challenging previous controversial records. Ecological niche models indicate that, under current climate conditions, L. delicatula might find suitable habitats primarily in specific high-altitude areas, while coastal and lower elevation zones generally show low suitability. Additionally, in Taiwan, most regions where the host plants of L. delicatula were recorded exhibited extremely low habitat suitability. Global climate change predictions suggest a possible northward shift in suitable habitats, and the existing limited suitable habitats in Taiwan may further decrease as climate change progresses.CONCLUSION However, concerning the relatively higher suitability for the species in particular mountainous areas, continuous monitoring and further research are recommended to conduct for better understanding the risk and preparing for potential future scenarios.
Эритроцитарный индекс RDW как маркер прогноза течения и исхода эмпиемы плевры, развившейся после пневмонии: проспективное когортное исследование
Authors: Фетлам Д. Л. , Чумаченко А. Г. , Данилов А. В. , Кузовлев А. Н. , Гречко А. В. , Писарев Владимир Митрофанович 
Published: August 2024

INTRODUCTION: The incidence of purulent destructive lung diseases including pleural empyema (PE) is increasing worldwide. Prognosis of the course and outcome is needed to personalize monitoring and care of PE patients as well as assess if previous coronavirus disease 2019 (COVID-19) may affect prognostic value of a biomarker. OBJECTIVE: Identification of inflammatory markers of course and outcome of PE developed after community-acquired pneumonia and determine the dependence of their prognostic value on previous COVID-19 in anamnesis. MATERIALS AND METHODS: The study included hospitalised post-community-acquired pneumonia PE patients (n = 216, age 18–87 years). Mann–Whitney U-criterion was used to assess significance of differences between groups. Odds ratios (OR) and 95 % confidence intervals (95% CI) were calculated. Kaplan–Meier method and log-rank test were used to determine differences in survival. RESULTS: Depending on the PE course (patients with and without fistula), the RDW (Red Cell Distribution Width) values were differed, as recorded on the third (p = 0.039), fifth (p = 0.018) and last days (p = 0.007) of hospitalization. Raised RDW value (Hazard Ratio (HR) = 2.4; 95 % confidence interval (CI) 1.0–5.6; р = 0.0454, log-rank test, n = 216) and C-reactive protein (CRP) level increase (HR = 3.5; 95% CI 1.4–8.5; р = 0.0036, log-rank test, n = 216) on the first day of hospitalization predicted the unfavourable outcome in the whole cohort of patients with PE. In patients with no PCR-proved COVID-19 in anamnesis, RDW value increased on the first day of hospitalization predicting unfavourable outcome (HR = 2.8; 95% CI 1.0–7.7; р = 0.0319, log-rank test, n = 135). In post-COVID-19 patients, increased CRP value and extended RDW level were not significantly associated with poor outcome. CONCLUSIONS: CRP level predicted mortality only in a whole cohort of patients with pleural empyema; the significance of prediction was lost in a subgroup of patients with a previous PCR-proved COVID-19. Increased RDW value on the first day of hospitalization predicted unfavourable outcome only in patients with no PCR-proved anamnestic COVID-19.
Частота и факторы риска развития послеоперационного делирия у детей при коррекции различных форм косоглазия: проспективное наблюдательное слепое исследование
Authors: Головатая Мария Вячеславовна , Мясникова В. В. , Сахнов С. Н. , Дереза С. В. 
Published: August 2024

INTRODUCTION: At present, the problem of emergence delirium in children during surgical correction of various forms of strabismus is still relevant, especially if the child has concomitant neurological pathology. OBJECTIVE: To assess the incidence and risk factors for the development of emergence delirium in children with and without concomitant neurological pathology after anesthesia with xenon or sevoflurane during surgical correction of various forms of strabismus. MATERIALS AND METHODS: 240 children who were operated on in a planned manner for the correction of strabismus. RESULTS: The incidence of emergence delirium was statistically significantly higher with sevoflurane anesthesia compared with xenon anesthesia (p < 0,01). The frequency of emergence delirium in children with concomitant neurological pathology is higher than in children without it (p < 0,05). There was a statistically significant difference in the frequency of emergence delirium in groups of children with concomitant neurological pathology under anesthesia with sevoflurane and under anesthesia with xenon (p < 0,05): under anesthesia with sevoflurane the frequency of emergence delirium was higher. In groups of children without concomitant neurological pathology, there was no statistically significant difference between anesthesia with sevoflurane and anesthesia with xenon (p > 0,05). CONCLUSIONS: Xenon anesthesia rarely causes the development of emergence delirium in children with concomitant neurological pathology during surgical correction of various forms of strabismus.
Проблемы ранней диагностики и коррекции белково-энергетической недостаточности у пациентов высокого операционно-анестезиологического риска в абдоминальной онкохирургии: обзор литературы
Authors: Лейдерман Илья Наумович , Медведев А. Ю. , Еремеева О. Г. , Зуков Р. А. 
Published: August 2024

INTRODUCTION: The share of patients with high surgical and anesthesia risk in the total structure of oncological patients is increasing every year. Patients with protein-energy malnutrition (PEM) are characterized by a high frequency of postoperative complications. Progression of PEM in patients with high surgical and anesthesia risk occurs faster due to both the oncologic process itself and severe comorbidity. OBJECTIVE: To summarize and analyze information on modern methods of early diagnosis and correction of PEM in abdominal oncosurgery patients at high operative and anesthetic risk in the perioperative period. MATERIALS AND METHODS: A systematic search and selection of publications was performed using the bibliographic databases PubMed and eLibrary. Studies published in the last 15 years (2008–2023) were analyzed to investigate the problem. Relevant studies were limited to systematic literature reviews, meta-analyses, randomized controlled trials, prospective observational studies, retrospective studies. RESULTS: The use of special assessment systems that identify different stages of PEM (CASC IN, miniCASCO, new cachexia staging scale 2018) in cancer patients at high surgical and anesthetic risk creates conditions for the implementation of a personalized approach to the selection of nutritional support program in the perioperative period. Assessment of nutritional status, including the analysis of laboratory markers of malnutrition (total protein, albumin, serum transferrin), as well as qualitative assessment of body composition using bioimpedance analysis contribute to the early detection of PEM and allow for maximum personalization of nutritional support and hydration of this patients. CONCLUSIONS: This narrative review has shown that currently it is rather difficult to formulate algorithms for diagnostics and correction of PEM in patients with high surgical and anesthesia risk in abdominal oncosurgery. Research is required to determine the effectiveness of different scales of PEM staging. A personalized approach to early diagnosis and correction of PEM is one of the most promising strategies to reduce the incidence of postoperative complications in this category of patients.
Correlation of Different Time Measurements of the Surgical Pleth Index with Postoperative Pain: A Prospective Observational Study
Authors: Hamed Mohamed Ahmed , Goda Abeer Shaban , Abdelghaffar Rana Ahmed , Algyar Mohammad Fouad , Mohamed Maha Ahmed , Abdelhady Mahdy Ahmed 
Published: August 2024

Background
The Surgical Pleth Index (SPI) has been introduced to monitor intraoperative pain under general anesthesia. This study aimed to determine the optimum time to measure intraoperative SPI, which correlates better with postoperative pain.

Methods
This prospective blind study collected data from female patients scheduled for elective abdominal hysterectomy under general anesthesia. SPI was recorded 5 minutes after the skin incision and 10 minutes before recovery. After recovery, upon the ability to communicate, all patients were asked to quantify their pain level. The primary outcome was determining which SPI measurement correlates more with postoperative pain.

Results
In the current study, we found a statistically significant correlation between SPI 10 min before recovery and the highest NRS (r=0.555, p-value <0.001). With the highest sensitivity and specificity, the cut-off value of SPI at 10 minutes before recovery to differentiate between moderate and severe pain was 57. On the other hand, no significant correlation was observed between SPI 5 min after skin incision and the highest NRS. Also, SPI 10 at minutes was significantly correlated with postoperative tramadol consumption, which was unlike SPI at 5 minutes.

Conclusion
SPI is a good indicator for nociception. Pre-arousal SPI score correlates more with postoperative pain scores and opioid consumption than SPI score after skin incision. Also, SPI 10 minutes before recovery value of 57 was the cut-off value, with the highest sensitivity and specificity, to differentiate between moderate and severe pain.
Investment Decision Making on Small-sized Enterprise: Case of the Aquaculture Fishery in Tuban Regency
Authors: Utami Tiwi Nurjannati , Subagyo Subagyo 
Published: August 2024

This study aims to analize the meaning, the decision making and the identification of fishery small entreprise investment. The study locates in Tuban Coastal–East Java Indonesia. It employs qualitative methods with case study. The data are collected through semi-structural interview, observation, and documentation. Techniques and time triangulation are used to check the validity of the data. CV. SAM is a fishery business on aquaculture of shrimp (vanname sp) and catfish (clarisas batracus sp), also makes probiotic and vitamins for fishes. The result shows that the entrepreneur interprets an investment as purchasing an asset to operation the entreprise. The owner decides on an investment for the purpose of expanding business and taking opportunity on the potential big market. Nevertheless, the enterprise identification several risks on their business. Firstly, operational risk; there are fish disease and increasing price of the feed and medicine on vanname that decreasing profit. Secondly, market risk; there are rejection or decline of selling price if the quality on low standart especilly for vanname on foreign markets. The entreprise anticipates operational risk by producing probiotics and vitamins for fish. On the market risks, the entrepreneur hasn’t any action to anticipate and to find solution especially for vanname. To develop the fishery small enterprise, the stakeholders need to support them in getting solution.
Smartphone-Based Smoking Cessation Intervention (OKquit) for Oklahoma Tobacco Helpline Users: Protocol for a Randomized Controlled Trial
Authors: Businelle Michael , Becerra Jessica , Witten Carl , Chen Sixia , Kezbers Krista , Beebe Laura A , Kendzor Darla E 
Published: August 2024

Background
Tobacco quitlines provide effective resources (eg, nicotine replacement therapy, smoking cessation counseling, and text and web-based support) for those who want to quit smoking in the United States. However, quitlines reach approximately only 1%-3% of people who smoke each year. Novel, smartphone-based, and low-burden interventions that offer 24/7 access to smoking cessation resources that are tailored to current readiness to quit may increase appeal, reach, and effectiveness of smoking cessation interventions.

Objective
This study will examine the efficacy of OKquit, a low-burden smartphone-based app for smoking cessation.

Methods
Approximately 500 people who smoke cigarettes and access the Oklahoma Tobacco Helpline (OTH) will be randomized to receive standard OTH care (SC) or SC plus the novel OKquit smartphone app for smoking cessation (OKquit). All participants will use a smartphone app to complete study surveys (ie, baseline, 27 weekly surveys, brief daily check-ins, and 27-week follow-up). Upon completion of daily check-ins and weekly surveys, participants will receive either trivia type messages (SC) or messages that are tailored to current readiness to quit smoking and currently experienced lapse triggers (OKquit). In addition, those assigned to receive the OKquit app will have access to on-demand smoking cessation content (eg, quit tips, smoking cessation medication tips). It is hypothesized that participants assigned to OKquit will be more likely to achieve biochemically verified 7-day point prevalence abstinence than those assigned to SC at 27 weeks post enrollment. In addition, participants who use more OTH resources (eg, more cessation coaching sessions completed) or more OKquit resources (eg, access more quit tips) will have greater biochemically verified smoking cessation rates.

Results
Data collection began in September 2022 and final follow-ups are expected to be completed by May 2025.

Conclusions
Data from this randomized controlled trial will determine whether the OKquit smartphone app combined with OTH care will increase smoking cessation rates over standard OTH care alone. If successful, OKquit could provide tailored intervention content at a fraction of the cost of traditional interventions. Furthermore, this type of low-burden intervention may offer a way to reach underserved populations of adults who smoke and want to quit.

Trial Registration
ClinicalTrials.gov NCT05539209; https://clinicaltrials.gov/study/NCT05539209

International Registered Report Identifier (IRRID)
DERR1-10.2196/56827
Karakteristik Fisik Dan Kandungan Antioksidan Bubur Instan Dari Beras Pratanak, Tepung Mocaf, Dan Bayam Merah
Authors: Hanifa Zulfa Nur 
Published: August 2024

Diabetes mellitus merupakan penyakit yang terjadi akibat gangguan metabolisme sehingga kadar gula darah diatas normal. Konsumsi pangan fungsional dapat mencegah terjadinya gangguan kesehatan seperti diabetes mellitus, karena pangan fungsional mengandung senyawa antioksidan yang baik bagi tubuh. Bubur instan berbahan dasar beras pratanak dan tepung mocaf yang difortifikasi bayam merah dapat menjadi pangan fungsional yang sangat baik untuk kesehatan. Pembuatan bubur instan dilakukan dengan 5 formulasi perlakuan perbandingan beras pratanak dan tepung mocaf serta 3 perlakuan fortifikasi bayam merah. Pengujian terhadap karakteristik fisik dan kandungan antioksidan diperlukan untuk mengetahui keberhasilan bubur instan sebagai pangan fungsional. Berdasarkan hasil pengujian fisik, bubur instan dengan perlakuan perlakuan T4K2 yaitu bubur instan dengan perbandingan tepung beras pratanak dengan tepung mocaf 70% : 30% dengan penambahan tepung bayam merah 2% merupakan bubur instan dengan perlakuan terbaik dan memiliki nilai aktivitas antioksidan 73,2764 μg/ml yang berarti termasuk ke dalam antioksidan kuat.