bluuebunny/crossref_metadata_2025_split huggingface dataset:
How User‐Created‐Content (UCC) Service Quality Influences User Satisfaction and Behaviour
Authors: Kim Min Sun , Moon Yun Ji , Kim Woo Gon 
Published: September 2012

AbstractThe Internet is undergoing a gradual shift from Web 1.0‐based user interaction to Web 2.0, which has a greater emphasis on user‐created content (UCC) and user collaboration (Hsu & Hsu, 2008). Since existing models of e‐service quality are likely deficient under Web 2.0, we build on them and test a new framework using 319 users who frequented UCC websites. Among other findings, three service elements together explained 44% of the variance in user satisfaction and 33% of the variance in website usage. These elements were: the provider‐user relationship (customization and participation), the relationship among users (e.g., user empowerment), and user compensation. Copyright © 2012 ASAC. Published by John Wiley & Sons, Ltd.
Parallel classification and feature selection in microarray data using SPRINT
Authors: Mitchell Lawrence , Sloan Terence M. , Mewissen Muriel , Ghazal Peter , Forster Thorsten , Piotrowski Michal , Trew Arthur 
Published: September 2012

SUMMARYThe statistical language R is favoured by many biostatisticians for processing microarray data. In recent times, the quantity of data that can be obtained in experiments has risen significantly, making previously fast analyses time consuming or even not possible at all with the existing software infrastructure. High performance computing (HPC) systems offer a solution to these problems but at the expense of increased complexity for the end user. The Simple Parallel R Interface is a library for R that aims to reduce the complexity of using HPC systems by providing biostatisticians with drop‐in parallelised replacements of existing R functions. In this paper we describe parallel implementations of two popular techniques: exploratory clustering analyses using the random forest classifier and feature selection through identification of differentially expressed genes using the rank product method. Copyright © 2012 John Wiley & Sons, Ltd.
The response of semi‐arid ephemeral wetland plants to flooding: linking water use to hydrological processes
Authors: Drake Paul L. , Coleman Blaire F. , Vogwill Ryan 
Published: September 2012

ABSTRACTEvergreen plants inhabiting ephemeral wetlands endure long dry spells interspersed with periods of flooding (or inundation). Inundation events are likely to be important for plant water use and growth, but few studies have linked the physiology of plants to hydrological processes during flood. We investigated the link between changes in the soil physical environment and plant water use traits in a stand of Casuarina obesa Miq and Melaleuca strobophylla Barlow trees during a controlled inundation event at Toolibin Lake. Toolibin Lake is an internationally recognized ephemeral wetland, which is under threat from altered hydrology and salinization. During flood, the velocity of water movement through the clay‐dominated soil profile suggested that macropores and plant root preferential flow paths aided water distribution. C. obesa was more capable than M. strobophylla to capitalize on the inundation event, suggesting preferential use of macropore water and a concentration of roots near the soil surface. Sap flux (QS) and tree diameter (ϕ) were interdependent and there was a correlation between increasing ϕ and increasing pre‐dawn leaf water potential (Ψpd). These observations link the movement of water through the soil profile with changes in tree water use and tree girth in C. obesa and M. strobophylla. Changes in the soil physical environment observed in this study also highlight the risks associated with hydrological management in salinity‐affected ecosystems. Although floods provide fresh water to the root zone of trees, the resulting recharge may also elevate saline groundwater into the region of plant water uptake. Copyright © 2012 Commonwealth of Australia
Complexes of Trinuclear Macrocyclic Copper(I) and Silver(I) 3,5‐Bis(Trifluoromethyl)Pyrazolates with Ketones
Authors: Titov Alexey A. , Filippov Oleg. A. , Bilyachenko Alexey N. , Smol'yakov Alexander F. , Dolgushin Fedor M. , Belsky Vitaly K. , Godovikov Ivan A. , Epstein Lina M. , Shubina Elena S. 
Published: September 2012

AbstractThe interaction of the copper [{3,5‐(CF3)2Pz}Cu]3 and silver [{3,5‐(CF3)2Pz}Ag]3 macrocycles {3,5‐(CF3)2Pz = 3,5‐bis(trifluoromethyl)pyrazole} with 2‐butanone and benzophenone was investigated for the first time by IR and NMR spectroscopy and single‐crystal X‐ray diffraction. New, low‐frequency IR bands for ν(CO)bond (in hexane) were observed and the composition (1:1) of all the complexes was determined. These data as well as the 13C NMR chemical shifts (in CD2Cl2) for the complexes of aliphatic and aromatic ketones evidence that the oxygen atom of the CO group is the sole coordination site in both complexes and that the Ph rings of benzophenone do not take part in complexation in solution. The thermodynamic parameters for the formation of the complexes increase on going from the copper to silver macrocycle and grow slightly from butanone to benzophenone. The same coordination site (CO group) was found in the crystal structure. However, the complexes with benzophenone are formulated as 1:2 complexes and feature a wedge‐shaped sandwich structure, but [AgL]3 also forms a 1:1 complex with triple coordination of the oxygen atom to each Ag atom. Analysis of the IR spectroscopic data for the complexes in crystal form and in solution revealed that in solution the [Ph2CO][ML]3 complexes also have triple coordination of the CO oxygen atom to the metals of both the Cu and Ag macrocycles. The structure of the 1:1 complex features no interaction between the Ph rings and the macrocycle in contrast to sandwiches (1:2) with shortened distances Ag···C (Ph). It was found that the sandwich complexes pack as infinite stacks, whereas the complex with triple coordination (1:1) forms only cofacial dimers.
An Amperometric Immunosensor Based on a Gold Nanoparticle‐Diazonium Salt Modified Sensing Interface for the Detection of HbA1c in Human Blood
Authors: Liu Guozhen , Iyengar Sridhar G. , Gooding J. Justin 
Published: September 2012

AbstractAn amperometric immunosensor for glycosylated hemoglobin (HbA1c) is reported. A glassy carbon electrode is modified with gold nanoparticles (AuNPs) bearing a ferrocene derivative and a glycosylated pentapeptide (GPP) as an epitope to which anti‐HbA1c IgG can selectively bind. The rest of the electrode is passivated with an oligo(ethylene oxide) species to give the electrode resistant to nonspecific adsorption of proteins. Complexation of anti‐HbA1c IgG with the surface bound epitope resulted in attenuation of the ferrocene electrochemistry. The immunosensor can detect HbA1c in the range of 4.6 %–15.1 % of total hemoglobin in human blood by a competitive inhibition assay.
New genetic findings in parotid gland pleomorphic adenomas
Authors: Wemmert Silke , Willnecker Vivienne , Brunner Christian , Wenzel Gentiana Ioana , Sauter Birgit , Meinelt Heike , Bartholmé Nadia , Saada Carolin , Bohle Rainer Maria , Urbschat Steffi , Schick Bernhard 
Published: September 2012

BackgroundDespite numerous studies, the tumor biology of pleomorphic adenomas, the most common salivary gland tumors, is still not completely defined. In order to identify further candidate genes important for tumor biology of pleomorphic adenomas, extended cytogenetic and molecular analysis are mandatory.MethodsWe performed a detailed molecular cytogenetic analysis using comparative genomic hybridization (CGH) followed by fluorescence in situ hybridization (FISH) with probes for chromosome X, 16p, 17, and 20 on a large cohort of pleomorphic adenomas (n = 29).ResultsWe could confirm previously described deletions in pleomorphic adenomas affecting 16p, 17, 20q, and 22 by FISH and/or CGH analysis. Moreover, our CGH study revealed novel candidate regions on 8p23.1pter, 9p, 10q25.1q25.3, and 11q24qter in the series of analyzed pleomorphic adenomas.ConclusionOur present study reveals new insights in novel candidate regions implicated in pleomorphic adenoma tumorigenesis which should be considered in further molecular studies. © 2012 Wiley Periodicals, Inc. Head Neck 35: 1431–1438, 2013
Prognostic value of vascular endothelial growth factor in patients with head and neck cancer: A meta‐analysis
Authors: Zang Jian , Li Chen , Zhao Li‐Na , Shi Mei , Zhou Yong‐Chun , Wang Jian‐Hua , Li Xin 
Published: September 2012

BackgroundThe correlation between vascular endothelial growth factor (VEGF) overexpression and the clinical outcome of head and neck cancer remains inconclusive. This meta‐analysis aimed to evaluate the prognostic value of VEGF in patients with head and neck cancer.MethodsWe searched Ovid MEDLINE, EMBASE and 2 Chinese science databases in order to enroll all eligible articles. Forty‐seven studies were included in this meta‐analysis. All results were evaluated by the random‐effects model.ResultsVEGF overexpression is significantly associated with worse overall survival (OS; hazard ratio [HR], 1.89; 95% confidence interval [CI], 1.61–2.22) and progression‐free survival (PFS; HR, 1.68; 95% CI, 1.33–2.12). Subgroup analysis reveals that VEGF overexpression is a significant poor predictor for nasopharyngeal cancer (HR, 1.66; 95% CI, 1.30–2.12) and salivary gland cancer (HR, 3.32; 95% CI, 1.61–6.84).ConclusionOur meta‐analysis supports that VEGF overexpression is an available poor predictor for patients with head and neck cancer. © 2012 Wiley Periodicals, Inc. Head Neck 35: 1507–1514, 2013
Hypopharyngeal paraganglioma with a paraneoplastic neurologic syndrome: A case report
Authors: Tian Huiqin , Chen Zhibin , Wang Cong , Xing Guangqian 
Published: September 2012

BackgroundWe report an extremely rare case of hypopharyngeal paraganglioma that was accompanied by a paraneoplastic neurologic syndrome (PNS).MethodsThe clinical, radiological, and histopathologic findings of the patient are presented.ResultsA 49‐year‐old woman presented with a history of progressive asthenia of the lower extremities persisting for 2 months. She also experienced numbness and sensory disturbance of the left lower limbs, 2 epileptic seizures, and constipation during the period. Clinical and radiological evaluations suggested the diagnosis of encephalomyelitis in combination with a left hypopharyngeal mass lesion. The patient underwent surgical excision of the lesion, which subsequently resolved her symptoms. Postoperative pathology was consistent with a paraganglioma. There was no evidence of local recurrence or distant metastasis of the tumor on recent follow‐up.ConclusionsTo the best of our knowledge, our patient represents the third reported case of hypopharyngeal paraganglioma, and the only one to have a paraneoplastic syndrome. © 2012 Wiley Periodicals, Inc. Head Neck 35: E304–E307, 2013
Perirhinal cortex represents nonspatial, but not spatial, information in rats foraging in the presence of objects: Comparison with lateral entorhinal cortex
Authors: Deshmukh Sachin S. , Johnson Jeremy L. , Knierim James J. 
Published: September 2012

AbstractThe medial temporal lobe (MTL) is involved in mnemonic processing. The perirhinal cortex (PRC) plays a role in object recognition memory, while the hippocampus is required for certain forms of spatial memory and episodic memory. The lateral entorhinal cortex (LEC) receives direct projections from PRC and is one of the two major cortical inputs to the hippocampus. The transformations that occur between PRC and LEC neural representations are not well understood. Here, we show that PRC and LEC had similarly high proportions of neurons with object‐related activity (PRC 52/94; LEC 72/153), as expected from their locations in the “what” pathway into the hippocampus. However, LEC unit activity showed more spatial stability than PRC unit activity. A minority of LEC neurons showed stable spatial firing fields away from objects; these firing fields strongly resembled hippocampal place fields. None of the PRC neurons showed this place‐like firing. None of the PRC or LEC neurons demonstrated the high firing rates associated with interneurons in hippocampus or medial entorhinal cortex, further dissociating this information processing stream from the path‐integration based, movement‐related processing of the medial entorhinal cortex and hippocampus. These results provide evidence for nonspatial information processing in the PRC‐LEC pathway, as well as showing a functional dissociation between PRC and LEC, with more purely nonspatial representations in PRC and combined spatial‐nonspatial representations in LEC. © 2012 Wiley Periodicals, Inc.
Combined analysis of three lynch syndrome cohorts confirms the modifying effects of 8q23.3 and 11q23.1 in MLH1 mutation carriers
Authors: Talseth‐Palmer Bente A. , Wijnen Juul T. , Brenne Ingvild S. , Jagmohan‐Changur Shantie , Barker Daniel , Ashton Katie A. , Tops Carli M. , Evans Tiffany‐Jane , McPhillips Mary , Groombridge Claire , Suchy Janina , Kurzawski Grzegorz ,   The Dutch Cancer Genetics Group, Spigelman Allan , Møller Pål , Morreau Hans M. , Van Wezel Tom , Lubinski Jan , Vasen Hans F.A. , Scott Rodney J. 
Published: September 2012

AbstractTwo colorectal cancer (CRC) susceptibility loci have been found to be significantly associated with an increased risk of CRC in Dutch Lynch syndrome (LS) patients. Recently, in a combined study of Australian and Polish LS patients, only MLH1 mutation carriers were found to be at increased risk of disease. A combined analysis of the three data‐sets was performed to better define this association. This cohort‐study includes three sample populations combined totaling 1,352 individuals from 424 families with a molecular diagnosis of LS. Seven SNPs, from six different CRC susceptibility loci, were genotyped by both research groups and the data analyzed collectively. We identified associations at two of the six CRC susceptibility loci in MLH1 mutation carriers from the combined LS cohort: 11q23.1 (rs3802842, HR = 2.68, p ≤ 0.0001) increasing risk of CRC, and rs3802842 in a pair‐wise combination with 8q23.3 (rs16892766) affecting age of diagnosis of CRC (log‐rank test; p ≤ 0.0001). A significant difference in the age of diagnosis of CRC of 28 years was observed in individuals carrying three risk alleles compared to those with 0 risk alleles for the pair‐wise SNP combination. A trend (due to significance threshold of p ≤ 0.0010) was observed in MLH1 mutation carriers towards an increased risk of CRC for the pair‐wise combination (p = 0.002). This study confirms the role of modifier loci in LS. We consider that LS patients with MLH1 mutations would greatly benefit from additional genotyping of SNPs rs3802842 and rs16892766 for personalized risk assessment and a tailored surveillance program.
Mortality audit of the Finnish cervical cancer screening program
Authors: Lönnberg Stefan , Nieminen Pekka , Luostarinen Tapio , Anttila Ahti 
Published: September 2012

AbstractIncidence‐based evaluations of cervical cancer screening programs have suggested age‐specific impacts and there is uncertainty regarding the effectiveness of screening outside the ages of 30–60 years. We audited the screening histories of cervical cancer deaths and conducted a case‐control evaluation of the effectiveness of organized screening in different ages with mortality as outcome. We included all 506 cervical cancer deaths in Finland in 2000–2009 due to cancers diagnosed in 1990 or later, and 3,036 controls matched by age at diagnosis to the cases. Squamous cell carcinoma constituted 59% of the cases, adenocarcinomas 29%, and the remaining 12% were other specified and unspecified cervical malignancies. Most deaths (54%) were due to cancers diagnosed more than 5 years after last screening invitation, 24% were diagnosed among nonattenders and only 14% of deaths occurred among women who had attended invitational screening. The risk reduction associated with attending a single program screen at an age below 40 was nonsignificant (OR 0.70; 95% CI 0.33–1.48), while clear risk reductions were observed after screening at the age of 40–54 (OR 0.33; CI 0.20–0.56) and 55–69 (OR 0.29; CI 0.16–0.54). This study also provides some indication of a long‐lasting additional effect of screening at the age of 65. Possible avenues for improving the effectiveness of the Finnish screening program include efforts to increase attendance and an extension of the target ages to include 65‐to 69‐year‐old women. The potential benefit of increasing the sensitivity of the screening test or shortening the screening interval is smaller.
Effects of irradiation on osteoblast‐like cells on different titanium surfacesin vitro
Authors: Li Yumei , Ma Wei , Feng Zhihong , Wang Zhijun , Zha Nianbao , Deng Banglian , Zhao Yimin 
Published: September 2012

AbstractThe aim of this study was to investigate the effects of irradiation on adhesion ability, proliferation, and differentiation of MC3T3‐E1 cells on microarc oxidation (MAO) titanium surfaces and polished titanium (PT) surfaces. MC3T3‐E1 cells were exposed to a single dose at 2, 4, 6, 8, or 10 Gy using a60Co source, with tissue culture polystyrene plates chosen as controls. On all surfaces, irradiation resulted in a dose‐dependent decrease in cellular proliferation. At 4 Gy dose, the cell proliferation of cells decreased by 17.8% on MAO and 18.6% on PT surfaces, respectively, compared with nonirradiated controls. Cells exposed to 8 Gy dose showed significant inhibition in collagen secretion and osteogenesis‐related genes expression (OSX, COL‐Iα1, and OCN). In contrast, irradiation increased cell adhesion to three surfaces dose dependently. It was also demonstrated that cells on MAO surface showed higher adhesion and collagen secretion than on PT surface at different radiation doses. This study revealed the effects of irradiation on osteoblastsin vitroon two titanium surfaces. MAO surface could be used in dental implants in irradiated bone due to enhanced adhesion ability and collagen secretion in osteoblasts. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 9–17, 2013.
Platelet‐rich plasma‐loaded chitosan scaffolds: Preparation and growth factor release kinetics
Authors: Kutlu Burak , Tiğlı Aydın Rahime Seda , Akman Abdullah C. , Gümüşderelioglu Menemşe , Nohutcu Rahime M. 
Published: September 2012

AbstractThe aim of this study is to compare the effects of different platelet‐rich plasma (PRP) preparation methods on platelet activity and to investigate the growth factor (GF) release kinetics from PRP‐loaded chitosan scaffolds for tissue engineering applications. Flow cytometry analysis showed that centrifugation processes used for PRP preparation did not cause significant effect on platelet activation levels by means of markers investigated. Two different methods were used to prepare PRP‐loaded chitosan scaffolds: (i) PRP was added to chitosan gel before freeze‐drying to prepare scaffolds called as “GEL” and (ii) PRP was embedded to freeze‐dried chitosan scaffolds to prepare scaffolds called as “SPONGE.” In addition, nonactivated PRP and PRP activated with type‐I collagen were used as control groups. Scanning electron microscopy images demonstrated that, in GEL group, there is no deterioration on the scaffolds porous, 3D, and interconnected structure. GF release kinetics was determined by enzyme‐linked immunosorbent assay for platelet‐derived GF‐BB, transforming GF‐β1, and insulin‐like GF‐1. A sustained release of GFs was achieved in GEL group while a sharp burst release was observed for all the GFs from the SPONGE groups. Moreover, platelet‐derived GF‐BB, insulin‐like GF‐1, and transforming GF‐β1 releases were prolonged to 20 days in GEL groups, and the biological activities of all GFs released from GEL and SPONGE scaffolds were preserved. This study demonstrated that chitosan scaffold that was called GEL could be an appropriate carrier for PRP applications by providing sustained release of GFs. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 101B: 28–35, 2013.
Human apolipoprotein E isoforms differentially affect bone mass and turnover in vivo
Authors: Dieckmann Marco , Beil F Timo , Mueller Brigitte , Bartelt Alexander , Marshall Robert P , Koehne Till , Amling Michael , Ruether Wolfgang , Cooper Jackie A , Humphries Steve E , Herz Joachim , Niemeier Andreas 
Published: September 2012

Abstract
The primary role of apolipoprotein E (apoE) is to mediate the cellular uptake of lipoproteins. However, a new role for apoE as a regulator of bone metabolism in mice has recently been established. In contrast to mice, the human APOE gene is characterized by three common isoforms APOE ε2, ε3, and ε4 that result in different metabolic properties of the apoE isoforms, but it remains controversial whether the APOE polymorphism influences bone traits in humans. To clarify this, we investigated bone phenotypes of apoE knock-in (k.i.) mice, which express one human isoform each (apoE2 k.i., apoE3 k.i., apoE4 k.i.) in place of the mouse apoE. Analysis of 12-week-old female k.i. mice revealed increased levels of biochemical bone formation and resorption markers in apoE2 k.i. animals as compared to apoE3 k.i. and apoE4 k.i., with a reduced osteoprotegerin (OPG)/receptor activator of NF-κB ligand (RANKL) ratio in apoE2 k.i., indicating increased turnover with prevailing resorption in apoE2 k.i. Accordingly, histomorphometric and micro–computed tomography (µCT) analyses demonstrated significantly lower trabecular bone mass in apoE2 than in apoE3 and apoE4 k.i. animals, which was reflected by a significant reduction of lumbar vertebrae maximum force resistance. Unlike trabecular bone, femoral cortical thickness, and stability was not differentially affected by the apoE isoforms. To extend these observations to the human situation, plasma from middle-aged healthy men homozygous for ε2/ε2, ε3/ε3, and ε4/ε4 (n = 21, n = 80, n = 55, respectively) was analyzed with regard to bone turnover markers. In analogy to apoE2 k.i. mice, a lower OPG/RANKL ratio was observed in the serum of ε2/ε2 carriers as compared to ε3/ε3 and ε4/ε4 individuals (p = 0.02 for ε2/ε2 versus ε4/ε4). In conclusion, the current data strongly underline the general importance of apoE as a regulator of bone metabolism and identifies the APOE ε2 allele as a potential genetic risk factor for low trabecular bone mass and vertebral fractures in humans. © 2013 American Society for Bone and Mineral Research