bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effectiveness and Cost-Effectiveness of Attachment Based Family Therapy for Young Adults with High Suicidal Ideation: Protocol of a Randomized Controlled Trial.
Authors: Bockting Claudi L. , Bosmans Guy , Bergers Nele , Gavan Luana , Hiligsmann Mickaël , de Beurs Derek , Molenberghs Geert , Wijnen Ben , Lokkerbol Joran , Spek Nadia van der 
Published: July 2024

Abstract

Young adult suicidality is worldwide a prevalent mental health problem and the number one cause of death, with devastating consequences for individuals and their families, and substantial economic costs. However, psychological and pharmacological treatments currently recommended in guidelines for treatment of high-risk youth for fatal suicide have limited effect. In line with the WHO’s recommendation to involve the family in treatment of these youth, Attachment Based Family Therapy (ABFT) was developed; a 16-week attachment and emotion-focused treatment, implemented in mental health care settings across various European countries in the past years, and is becoming increasingly popular among therapists. However, the (cost-)effectiveness of ABFT has not been studied in emerging adults. In the proposed pragmatic RCT we aim to evaluate the effectiveness and cost-effectiveness of ABFT compared to Treatment As Usual (TAU) on suicidality, as delivered in daily practice. This pragmatic multicenter study in the Netherlands and Belgium includes 12 participating sites. Participants are suicidal young adults (≥ 31 SIQ-JR score) between 18 and 25 years old who seek mental health treatment (n = 142) and their caregivers. The primary outcome is suicidality (SIQ-JR), with assessments at baseline, post-intervention (five months after baseline), three, six and twelve months after intervention. We predict that, compared to TAU, ABFT will lead to a stronger reduction in suicidality and will be more cost-effective, over the course of all timepoints. We also expect stronger decreases in depressive symptoms, given that suicidality is very common in individuals with depressive disorder, as well as more improvement in family functioning, autonomy, entrapment, and young adult attachment, in the ABFT condition. This study can contribute to improving the care for suicidal youngsters with high mortality risk. Treatment of suicidal emerging adults is understudied. The results will inform clinical guidelines and policy makers and improve treatment of suicidal emerging adults.
Study on Fracture Characteristics of Panel Asphalt Concrete Under Different Loading Rates
Authors: Yang Hanbing , He Jianxin , Yang Wu , Ding Xinyu , Chen Peng-peng , Liu Liang 
Published: July 2024

Abstract

The development of internal cracks in asphalt concrete can be seen as a sign of macro crack development. To explore the influence of different loading rates on the crack resistance of panel asphalt concrete, based on the theory of fracture mechanics, DIC digital image correlation technology was used as a test method for crack propagation of specimens. SCB (Semi-circular bending)tests were carried out on panel asphalt concrete with different loading rates (0.05, 0.2, 1, 3, 5,1 0 mm/min). The effects of different loading rates on the fracture index and full-field strain of asphalt concrete were analyzed. the results showed that when the loading rate increases from 0.05 mm/min to 10mm/min, the stress intensity factor (KIC) and fracture energy (Gf) increase, and the crack resistance index (CRI) shows the opposite law; With the increase of loading rate, the strain (Exx, Eyy) corresponding to the peak load gradually decreases. After reaching the peak strength, with the increase of load displacement, the crack of the sample gradually expands along the edge of the coarse aggregate at a low loading rate, and the crack penetrates the coarse aggregate at a high loading rate; There are obvious differences in crack morphology of asphalt concrete specimens under different loading rates, When the loading rate is 0.05-1 mm/min, the crack morphology is tortuously rising. When the loading rate is 3-10mm/min, the crack morphology develops almost vertically upward along the pre-crack. The results of the study can provide a reference for the development of cracks and fracture behavior of panel asphalt concrete under different loading rates.
Flavopiridol Suppresses Cell Proliferation and Metastasis and Induces Endoplasmic Reticulum Stress of Triple Negative Breast Cancer Cells
Authors: OZTURK Emel , Yakan Birkan , Hamurcu Zuhal 
Published: July 2024

Abstract

Background
As the number of breast cancer, which is the most common type of cancer, increases, alternative medicine has come to the fore. In this study, it was aimed to investigate the effect of flavopiridol on cell viability and apoptotic pathways associated with endoplasmic reticulum stress (ERS) on triple negative breast cancer cells.
Methods
In this study, cell viability test (MTS) was used for the effect of flavopiridol on the viability of triple negative breast cancer cells and clonogenic assay to determine its effect on colony formation. Migration assay showed migration rates of cells, expressions of proteins involved in apoptosis, ERS [Glucose regulated protein (GRP78), DNA damage inducible gene 153 (GADD153) and the apoptotic rate was measured with Annexin V.
Results
Flavopiridol was found to significantly reduce cell viability in the triple negative breast cancer cell lines in the 200nM-700nM dose range. We also found that FP induces apoptosis via ERS.
Conclusion
Our results showed that flavopiridol, when used in combination with other cytotoxic chemotherapeutic agents, will have promising potential for the treatment of patients with aggressive breast cancer, but further studies are needed in this regard.
Thalamic Purinergic Receptors: A Gateway to Modulating Fibromyalgia Pain via NLRP3 Inflammasome Signaling and Pyroptosis
Authors: Kamel Ahmed Seif El Din , Mohamed Maram M. , Zaki Hala F. 
Published: July 2024

Abstract

The high pain sensitivity in fibromyalgia (FM) is processed by the thalamus that presents as a key component in the pain pathway in FM patients. Noteworthy, Purinergic receptors, specifically P2X, are implicated in pain signaling and neuroinflammation via inflammasome signaling. However, there is no available data on the impact of pharmacological intervention on P2X receptor in thalamic pain transmission in FM. To investigate this aspect, the clinically tested P2X inhibitor, Suramin (SURM), was utilized. FM was induced over three days using Reserpine (1 mg/kg/day, s.c.), followed by a single dose of SURM (100 mg/kg, i.p.). At the molecular level, SURM countered the overexpression of P2X7 and P2X4 receptors accompanied by reduced NLRP3 inflammasome complex and pyroptotic markers like gasdermin-D. This was associated by the suppression of the p38-MAPK and NF-κB pathways, along with a decrease in pro-inflammatory cytokines and tumor necrosis factor-α as observed by increased CD86 expression on M1 microglia phenotype, a neuroinflammatory marker. Concurrently, blocking the P2X receptor shifted microglia polarization towards the M2 phenotype, marked by elevated CD163 expression, as a neuroprotective mechanism. This was outlined by increased neurotrophic and anti-inflammatory; IL-10 with normalization of disturbed neurotransmitters. Behaviorally, SURM ameliorated the heightened pain processing, as observed in mechanical and thermal pain tests. Furthermore, it lowered Reserpine-induced motor impairment in the rotarod and open-field tests. This improvement in the somatosensory experience was reflected in alleviating depressive-like behavior in the forced swimming test. These findings highlight the therapeutic potential of blocking thalamic P2X receptors in alleviating fibromyalgia symptoms.
Analyzing the Impact of Sphingolipid Metabolism Genes on bladder cancer Progression and Microenvironment for the Development of a Prognostic Signature
Authors: Peng Zechun , Yang Jie , Wu Tianshi 
Published: July 2024

Abstract

The role of sphingolipid metabolism (SM) in promoting the progression of bladder cancer (BLCA) and its impact on patient prognosis has been established. To improve therapeutic outcomes, it is essential to identify specific molecular pathways in BLCA and develop a predictive signature underlying SM-related genes. In this study, 430 BLCA samples were analyzed using univariate Cox regression to identify critical SM-relevant genes (SMGs) involved in BLCA development. LASSO regression analysis was then employed to reduce the possibility of overfitting. A multivariable Cox regression analysis was employed to develop a prognostic signature underlying SMGs, which was subsequently validated in a separate cohort. Our research revealed that dysregulated SM leads to worse prognosis in BLCA patients, and important prognostic genes (PCSK2, NFASC, NTF3, NR2F1, ATP13A2, SREBF1, GSDMB, and LGALS4) were identified. Using these SMGs, we developed a prognostic BLCA-risk model that effectively predicted the prognosis of BLCA patients (AUC was 0.772 for the training cohort and 0.725 for the validation cohort). Interestingly, patients identified as high-risk by this model had a significantly more active immunological milieu, characterized by higher immune scores and increased 26 types of immune function and cell like NK cells, CD8+T cells, and cytolytic activity. These findings suggest that dysregulated SM may contribute to immune microenvironment dysregulation in BLCA. Our research provides a better awareness of the role of SM in the emergence of BLCA and has the potential to offer customized care to high-risk patients based on their SM-related gene expression signature.
A Low-Cost, Open-Source Cylindrical Couette Rheometer
Authors: Erni Makita , Hart John , Trumper David , Owens Crystal E. 
Published: July 2024

Abstract

Rheology, commonly simplified by the property of viscosity, describes the flow of all fluids, from food and plastics, to coatings, adhesives, and 3D printing inks. While viscometers adequately probe Newtonian (constant) viscosity, most fluids have complex viscosity, requiring tests over multiple shear rates, and transient measurements. As a result, rheometers are typically large, expensive, and require additional infrastructure (e.g., gas lines), rendering them inaccessible for regular use by many individuals, small organizations, and educators. Here, we introduce a low-cost (under USD$200 bill of materials) Open Source Rheometer (OSR), constructed entirely from thermoplastic 3D printed components and off-the-shelf electromechanical components. A sample fluid rests in a cup while a microstepping motor rotates a tool inside the cup, applying strain-controlled shear flow. A load cell measures reaction torque exerted on the cup, and viscosity is calculated. To establish the measurement range, the viscosity of four Newtonian samples of 0.1–10 Pa.s were measured with the OSR and compared to benchmark values from a laboratory rheometer, showing under 23% error. Building on this, flow curves of three complex fluids – a microgel (hand sanitizer), foam (Gillette), and biopolymer solution (1% xanthan gum) – were measured with a similar error range. A further stress relaxation test was demonstrated on the biopolymer solution. The OSR cost is ∼1/25th that of commercially available devices with comparable minimum torque (200 µN.m), and provides a platform for further innovation in open-source rheometry.
Sensitivity to change of the COVID-19 Anxiety Syndrome Scale among Canadian dentists
Authors: Seth Rachita , Madathil Sreenath A. , Siqueira Walter L. , McNally Mary , Quinonez Carlos R. , Glogauer Michael , Allison Paul 
Published: July 2024

Abstract

Introduction:
There is a need to further validate the COVID-19 Anxiety Syndrome Scale (C-19ASS) by evaluating its sensitivity to change over time.
Aim
To estimate the sensitivity to change over time of the C-19ASS in a sample of Canadian dentists. To estimate the effect of age, sex, practice type and vaccination on the anxiety levels of dentists in Canada.
Methods
Longitudinal data were used from a prospective cohort study conducted to estimate the incidence of COVID-19 among dentists practising in Canada. Mixed effects ordinal logistic regression models were used to estimate the association between total C-19ASS scores and follow-up time in the study and COVID-19 case counts in the province of practice of the participants during the 14 days prior to completing the C-19ASS questionnaire. Mixed effects models were utilized to identify the factors associated with COVID-19 related anxiety.
Results
The odds of being in a more severe category of anxiety were reduced by 26% (OR 0.74, 95% C.I. 0.72–0.76) with every 30-day increase in the follow-up time. The odds of being in a more severe category of anxiety increased by 20% (Odds Ratio = 1.20, 95% C.I.= 1.12–1.27) with each 10,000 COVID-19 case increase in the dentists’ work province during the 14 days prior to data collection. Age, sex and practice type were significantly associated with COVID-19 related anxiety in dentists.
Conclusion
The C-19ASS is sensitive to change over time and with external anxiety stimuli. COVID-19 related anxiety among dentists was associated with age, sex and practice type.
Resistance of rice genotypes to lesser cornstalk borer Elasmopalpus lignosellus (Lepidoptera: Pyralidae)
Authors: Siqueira José Claudino , Filho Rafael Danillo Araújo Costa , Barros Wanderson Fernandes , Silva Anderson Rodrigo da , Almeida André Cirilo de Sousa , Barrigossi José Alexandre de Freitas , Jesus Flavio 
Published: July 2024

Abstract

The lesser cornstalk borer Elasmopalpus lignosellus (Zeiler 1848) (Lepidoptera: Pyralidae) is one of the most harmful pests of upland rice and others several economically important crops in Brazil. The lesser cornstalk borer is a pest that damages the newly emerged rice plant, reducing the "stand" and often requires replanting. The objective of this work was to identify source of resistance in rice genotypes to E. lignosellus. Thirty-day-old rice plants were infested with newly emerged larvae and evaluated to determine the intensity of damage: number of total stem, number of damaged stems, percentage of stems attacked, number of stems with dead heart symptom, percentage of stems with dead heart symptom and length of damage. Also the biological parameters of the lesser cornstalk borer: live larval number, larval weight and larval length were evaluated. The genotypes Canela de Ferro, Confiança, Esmeralda, Lajeado Ligeiro and Primavera are considered moderately resistant to E. lignosellus and can be used by rice producers as a component of Integrated Pest Management or used in rice breeding programs as a source of resistance to the lesser cornstalk borer.
Constructing and identifying an eighteen-gene Tumor Microenvironment Prognostic Model for Non-Small Cell Lung Cancer
Authors: Li Zaishan , Meng Zhenzhen , Xiao Lin , Du Jiahui , Jiang Dazhi , Liu Baoling 
Published: July 2024

Abstract

Background
The tumor microenvironment (TME) plays a crucial role in tumorigenesis and tumor progression. This study aimed to identify novel TME-related biomarkers and develop a prognostic model for patients with non-small-cell lung cancer (NSCLC).
Methods
After downloading and preprocessing data, we classified the molecular subtypes using the "NMF" R package. We performed survival analysis and quantified immune scores between clusters. A Cox proportional hazards model was then constructed, and its formula was produced. We assessed model performance and clinical utility. A prediction nomogram was also constructed and validated. Additionally, we explored the potential regulatory mechanisms of our TME gene signature using Gene Set Enrichment Analysis (GSEA).
Results
From data processing and univariate Cox regression, 57 TME-related prognostic genes were identified. Two clusters (C1 and C2) with significant differences were established. Immune scores, including those for cytotoxic lymphocytes, endothelial cells, monocytic cells, myeloid dendritic cells, neutrophils, and T cells, showed significant differences between the subtypes. Through univariate Cox analysis, lasso regression, and multivariate Cox regression analysis, an 18-gene TME-related prognostic model was developed. This model accurately predicted survival outcomes in subgroups with varying clinical features. Finally, a nomogram was constructed, and its predictive accuracy was validated.
Conclusions
We developed a prognostic model based on TME-related genes in NSCLC. Our 18-gene TME signature can effectively predict the prognosis of NSCLC with high accuracy.
Case Report - A Safe Laparoscopic Technique for Complicated Appendicitis
Authors: Glasser James 
Published: July 2024

Abstract

A case of complicated appendicitisis presented to illustrate a safe laparoscopic appendectomy technique.
What makes extirpation so difficult in complicated appendicitis? Infection and tissue injury trigger release of cytokines, which attract the omentum and cause contiguous loops of bowel to adhere, effectively isolating the inflammatory locus. Surgical dissection must reverse this process.
Visualization is excellent in laparoscopy; however, an operator lacks tactile sensation; and when organs are fused together, touch is a valuable aid to accurate dissection. Injury to the adjacent organs (small bowel, colon, fallopian tubes, or ureter) may occur and require resection (cecectomy or hemicolectomy) and anastomosis.
What is needed is an operative technique that is safe and effective in these challenging situations, especially where the appendix is adherent to adjacent structures and encased in a cocoon of (highly vascularized) fibrous tissue, a phlegmon.
The technique presented is derived from open surgery. It is safe because dissection is performed directly upon the appendiceal wall. This procedure (utilized in complicated appendicitis) is contrasted with an alternate (simpler) technique applicable to uncomplicated appendicitis.
Feasibility and acceptance of KIDSCREEN-52 as a screening tool for unmet needs in children with rare inflammatory diseases
Authors: Satirer Özlem , Erbis Gabi , Heck Verena , Welzel Tatjana , Reiser Christiane , Gellner Anne-Kathrin , Benseler Susanne M. , Kümmerle-Deschner Jasmin 
Published: July 2024

Abstract

Background: Children living with rare diseases often face significant psychosocial challenges; recognizing and addressing these effectively is crucial. However, there is a paucity of comprehensive screening tools. This study aimed to assess the feasibility and acceptance of the comprehensive KIDSCREEN-52 tool in identifying unmet needs of children with rare inflammatory diseases and their caregivers and identifying factors associated with low health-related quality of life (HRQoL).
Methods A prospective single-center study of consecutive pediatric patients aged 8-18 with inflammatory diseases and their caregivers was performed to assess HRQoL utilizing the multidimensional KIDSCREEN-52 self-report and proxy tool. The validated KIDSCREEN-52 tool is available in 13 languages with corresponding Norm Data. It captures HRQoL across 10 domains including 52 inquiries. HRQoL of children with rare inflammatory diseases was described utilizing the multidimensional KIDSCREEN-52 self-report and proxy tool. The feasibility and acceptability of KIDSCREEN-52 was determined using a simple, dichotomous three item acceptance tool. Factors associated with low self-reported HRQoL were explored.
Results: A total of 104 participants, comprising 51 pediatric patients and their 53 caregivers, were included. The patients were 35 females and 16 males, with a median age of 16 years (range: 9-18). Among them, 25 (49%) had autoinflammatory diseases, 26 (51%) had rheumatic diseases. Mean values from self-reports and proxies were consistent with the Norm Data across all domains. Self-report and proxy assessments showed high-degree agreement. Patients reported lower HRQoL levels compared to the control population in nearly all domains. Both caregivers and children expressed strong acceptance of the KIDSCREEN-52 questionnaire's clarity, relevance, and adequacy. The overall completion rate was 75%, the mean completion time 17 minutes (range: 10-25). Factors associated with low HRQoL included female gender, adolescent age and evidence of a rheumatic disease.
Conclusion: The KIDSCREEN-52 demonstrated promise as a feasible and accepted tool for capturing the HRQoL and identifying unmet needs in children with rare inflammatory diseases. Its comprehensiveness and the availability in multiple languages with corresponding Norm Data, offers a unique opportunity to implement strategies to identify and address HRQoL challenges of children with rare diseases in routine clinical care.
NMES1 promotes lung adenocarcinoma progression by activating PI3K/AKT signaling pathway
Authors: Wu Qian , Wang Ning , Sun Chao , Zhang Ying , Han Jue-ming , Wang Hui 
Published: July 2024

Abstract

Focusing on the role of normal mucosa of esophagus specific 1 (NMES1) in LUAD, TCGA database was hereby first used to explore the expression and prognostic value of NMES1 in LUAD patients. qRT-PCR, Western blotting and immunohistochemistry were utilized to detect expression of NMES1. NMES1 expression was up-regulated in LUAD patients and LUAD cells. Subsequently, siRNA was utilized to transfect LUAD cells to knockdown NMES1. Proliferation, migration and invasion potential were evaluated by cell counting Kit-8, wound healing and transwell migration. Furthermore, experiments were also conducted to investigate the potential mechanisms. Finally, a transplanted tumor model was established to elucidate the functions of NMES1 on the tumorigenesis of LUAD cells. Knockdown of NMES1 notably inhibited proliferation, migration, cell cycle and tumor growth in xenografts. The findings demonstrated the efficiency of NMES1 in mediating the ROS level and Mitochondrial potential to promote PI3K/AKT signaling pathway.
Association between adverse childhood experiences and the risk of developing chronic headache in Chinese individuals: a RCSCD-TCM study in China evidence from a national survey
Authors: Lu Hong , Huang Daiqiang , Yu Hegao , Li Cuiyin , Wen Shuangshuang , Zhao Dizhou 
Published: July 2024

Abstract

Background:
Chronic headache (CH) is a widespread problem in clinical practice. The causes of the disease are very complex and varied, including organic, functional and even psychogenic causes. Adverse childhood experiences (ACEs) are related to several psychogenic neurological disorders. However, whether the number of ACEs is associated with the risk of developing CH has not been well established. Therefore, in this study, we aimed to identify the association between the number of ACEs and the risk of developing CH in the general population using a Chinese national survey.
Methods:
Participants who reported experiencing headache in two consecutive waves of investigation from the China Health and Retirement Longitudinal Study (CHARLS) were defined as having CH and were included in this study. The life history information of the participants was then matched. Twelve types of ACEs were identified from the life history information. The participants were divided into four classes according to the number of ACEs. The association between the number of ACEs and the risk of developing CH was analysed using logistic regression.
Results:
A total of 654 (14.77%) of 4427 participants were diagnosed with CH within 9 years of the survey. After adjusting for various confounding factors, compared to that associated with Class 1 (ACEs = 0), the OR for CH associated with Class 3 (ACEs = 2) was 1.68 (1.30 ~ 2.19; P < 0.001) and the OR for CH associated with Class 4 (ACEs > 2) was 2.62 (2.04 ~ 3.38; P < 0.001). Further analysis of different types of ACEs demonstrated that physical abuse, household mental illness and five other types of ACEs were associated with the risk of developing CH. Subgroup analysis demonstrated similar results without any interactions.
Conclusions:
Exposure to ACEs was associated with a greater risk of developing CH, indicating the importance of early intervention for individuals with ACEs to mitigate the detrimental impact of ACEs on CH and to promote health.