bluuebunny/crossref_metadata_2025_split huggingface dataset:
FoxO Transcription Factors Support Oxidative Stress Resistance in Human Chondrocytes
Authors: Akasaki Yukio , Alvarez‐Garcia Oscar , Saito Masahiko , Caramés Beatriz , Iwamoto Yukihide , Lotz Martin K. 
Published: September 2014

ObjectiveA major signaling pathway that regulates cellular aging is the insulin/insulin‐like growth factor 1 (IGF‐1)/phosphatidylinositol 3‐kinase (PI3K)/Akt/FoxO transcription factor axis. We previously observed that FoxO transcription factors are dysregulated in aged and OA cartilage. The objective of this study was to investigate the impact of down‐regulated FoxO transcription factors on chondrocytes.MethodsSmall interfering RNAs (siRNAs) targeting FOXO1 (siFOXO1) and FOXO3 (siFOXO3) were transfected into human articular chondrocytes. Cell viability following treatment with the oxidant tert‐butyl‐hydroperoxide (tBHP) was measured by MTT assay. Caspase 3/7 activation and apoptotic cells were examined. Gene and protein expression of antioxidant proteins and autophagy‐related proteins and changes in inflammatory mediators following treatment with interleukin‐1β were assessed. Cells transfected with FOXO plasmids were also analyzed.ResultsCell viability was significantly reduced by siFOXO after treatment with tBHP. Apoptosis accompanied by caspase activation was significantly increased in siFOXO‐transfected chondrocytes. Knockdown of FOXO1 and FOXO1+3 resulted in significant reductions in levels of glutathione peroxidase 1 (GPX‐1), catalase, light chain 3 (LC3), Beclin1, and sirtuin 1 (SIRT‐1) proteins following treatment with tBHP. In contrast, the constitutive active form of FOXO3 increased cell viability while inducing GPX‐1, Beclin1, and LC3 in response to tBHP. Expression and production of ADAMTS‐4 and chemerin were significantly increased in siFOXO‐transfected chondrocytes.ConclusionReduced expression of FoxO transcription factors in chondrocytes increased susceptibility to cell death induced by oxidative stress. This was associated with reduced levels of antioxidant proteins and autophagy‐related proteins. Our data provide evidence for a key role of FoxO transcription factors as regulators of chondrocyte oxidative stress resistance and tissue homeostasis.
The Impact of Depression on Self–Other Discrepancies in Decision Making
Authors: Garcia‐Retamero Rocio , Okan Yasmina , Maldonado Antonio 
Published: September 2014

ABSTRACTAlthough there is a small but growing body of literature on how people make risky decisions for others and predict others' decisions, results seem to be contradictory. The authors contribute to the understanding of these mixed results by investigating how depression affects self–other discrepancies in decision making and the psychological processes that underlie these discrepancies. In an experiment, depressed and nondepressed individuals read a series of scenarios involving decisions about health, money, and interpersonal relationships. They then indicated which of two options they would choose for themselves or for another person, or predicted which option this person would choose for himself or herself. Finally, participants reported benefits and drawbacks of the decisions (i.e., cognitions) and feelings about risk. Depressed individuals were less prone to bias when they predicted others' decisions than nondepressed individuals. Feelings about risk played a key role in determining the direction and the magnitude of this bias. In contrast, both depressed and nondepressed individuals showed bias when they made decisions for others. This bias affected their decisions in opposing ways and was determined by cognitions. This bias is consistent with literature showing that depression is associated with an increased sensitivity to social risks. The authors provide a theoretical explanation of self–other discrepancies in decision making in depressed and nondepressed individuals and conclude that the results support the assumption that depression is associated with psychological processes whose role is to increase sensitivity to social threats rather than with a more general negative bias in cognitive functioning. Copyright © 2014 John Wiley & Sons, Ltd.
Poloxamer surfactant preserves cell viability during photoacoustic delivery of molecules into cells
Authors: Sengupta Aritra , Dwivedi Nishant , Kelly Sean C. , Tucci Lara , Thadhani Naresh N. , Prausnitz Mark R. 
Published: September 2014

ABSTRACTEfficient intracellular delivery of molecules is needed to modulate cellular behavior for laboratory and medical applications, but is often limited by trade‐offs between achieving high intracellular delivery and maintaining high cell viability. Here, we studied photoacoustic delivery of molecules into cells by exposing DU145 human prostate carcinoma cells to nanosecond laser pulses in the presence of carbon black nanoparticles. Under strong laser exposure conditions, less than 30% of cells were viable and exhibited uptake. Addition of poloxamer surfactant at those laser exposure conditions increased cell viability to almost 90%, with intracellular uptake in >80% of cells. This remarkable increase in efficiency of intracellular delivery and cell viability may be attributed to enhanced cell membrane resealing by poloxamer surfactant after photoacoustic delivery. While F‐68 poloxamer was effective, the larger, more‐hydrophobic F‐127 poloxamer provided the best results. There was no significant protective effect from addition of Ca2+, BAPTA‐AM, ATP, fetal bovine serum or glycine betaine, which were expected to promote active cell membrane repair mechanisms and other active intracellular protective processes. We conclude that poloxamer surfactant preserves cell viability during photoacoustic delivery of molecules into cells, thereby enabling highly efficient intracellular delivery. Biotechnol. Bioeng. 2015;112: 405–415. © 2014 Wiley Periodicals, Inc.
miR181c promotes apoptosis and suppresses proliferation of metanephric mesenchyme cells by targeting Six2 in vitro
Authors: Lv Xiaoyan , Mao Zhaomin , Lyu Zhongshi , Zhang Pengzong , Zhan Alan , Wang Jian , Yang Hui , Li Mi , Wang Honglian , Wan QianYa , Wei Hongyuan , Wang Ming , Wang Nian , Li Xun , Liu Yi , Zhao Hui , Zhou Qin 
Published: September 2014

Increasingly recognized importance has been assumed for microRNA (miRNA) in the regulation of the delicate balance of gene expression. In our study, we aimed to explore the regulation role of miR181c towards Six2 in metanephric mesenchyme (MM) cells.Bioinformatics analysis, luciferase assay and semi‐quantitative real‐time (RT) PCR, subsequently RT PCR, Western blotting, 5‐ethynyl‐2′‐deoxyuridine cell proliferation assay, Cell Counting Kit‐8 assay, immunofluorescence and flow cytometry, were employed to verify the modulation function of miR181c on Six2 in the mK3 MM cell line that is one kind of MM cells.miR181c was predicted to bind the 3′ untranslated region of Six2 by bioinformatics analysis, which was subsequently validated by the in vitro luciferase reporter assay. Moreover, transfection of miR181c mimic can decrease the expression of Six2 both in mRNA and protein levels in mK3 cells. Still, ectopic expression of miR181c inhibits the proliferation, promotes the apoptosis and even makes the nephron progenitor phenotype lose mK3 cells.These results revealed the ability of a single miRNA–miR181c to downregulate the expression of Six2, restrain the proliferation and promote the apoptosis that even makes the nephron progenitor phenotype lose MM cells, suggesting a potential role of miR181c during the kidney development. Copyright © 2014 John Wiley & Sons, Ltd.
An Isocytidine Derivative with a 2‐Amino‐6‐methylpyridine Unit for Selective Recognition of the CG Interrupting Site in an Antiparallel Triplex DNA
Authors: Okamura Hidenori , Taniguchi Yosuke , Sasaki Shigeki 
Published: September 2014

AbstractSequence‐specific recognition of duplex DNA mediated by triple helix formation offers a potential basis for oligonucleotide therapy and biotechnology. However, triplex formation is limited mostly to homopurine strands, due to poor stabilization at CG or TA base pairs in the target duplex DNA sequences. Several non‐natural nucleosides have been designed for the recognition of CG or TA base pairs within an antiparallel triplex DNA. Nevertheless, problems including low selectivity and high dependence on the neighboring bases remain unsolved. We thus synthesized N2‐arylmethyl isodC derivatives and incorporated them into triplex‐forming oligonucleotides (TFOs) for the selective recognition of the CG base pair within antiparallel triplex DNA. It was shown that an isodC derivative bearing a 2‐amino‐6‐methylpyridine moiety (AP‐isodC) recognizes the CG base pair with high selectivity in antiparallel triplex DNA irrespective of the flanking base pairs.
Multiplexed Programmable Release of Captured DNA
Authors: Kennedy‐Darling Julia , Holden Matthew T. , Shortreed Michael R. , Smith Lloyd M. 
Published: September 2014

AbstractNucleic‐acid hybridization is widely used for the specific capture of complementary sequences from complex samples. It is useful for both analytical methodologies, such as array hybridization (e.g. transcriptome analysis, genetic‐variation analysis), and preparative strategies such as exome sequencing and sequence‐specific proteome capture and analysis (PICh, HyCCAPP). It has not generally been possible to selectively elute particular captured subsequences, however, as the conditions employed for disruption of a duplex can lack the specificity needed to discriminate between different sequences. We show here that it is possible to bind and selectively release multiple sets of sequences by using toehold‐mediated DNA branch migration. The strategy is illustrated for simple mixtures of oligonucleotides, for the sequence‐specific capture and specific release of crosslinked yeast chromatin, and for the specific release of oligonucleotides hybridized to DNA microarrays.
High glucose‐induced Galectin‐1 in human podocytes implicates the involvement of Galectin‐1 in diabetic nephropathy
Authors: Liu Yinghong , Long Luping , Yuan Fang , Liu Fuyou , Liu Hong , Peng Youming , Sun Lin , Chen Guochun 
Published: September 2014

AbstractThe diabetic milieu is believed to change the activity, or result in damage of podocytes—a key component of the glomerular filtration barrier and known to secrete matrix for glomerular basement membrane. This in turn contributes to diabetic nephropathy. However, how podocyte dysfunction is triggered in diabetic nephropathy remains ambiguous. Galectin‐1 belongs to Galectin family that bind to β‐galactoside residues of glycosylated proteins. We explored whether Galectin‐1 is dysregulated in diabetic nephropathy using three different techniques, namely real‐time polymerase chain reaction, western blotting, and immunofluorescent staining, to follow the expression of Galectin‐1 under high glucose levels in podocytes. High glucose consistently induced Galectin‐1 expression. Immunohistochemistry using a Galectin‐1‐specific antibody also showed elevated Galectin‐1 in renal tissues of diabetic patients with manifestation of nephropathy, indicating a correlation of Galectin‐1 overexpression with diabetic nephropathy. Upregulation of Galectin‐1 is associated with loss of podocin, which is important for the physiological function of podocytes and decreases in the renal tissues of diabetic nephropathy. Increased Galectin‐1 is a causal event for the high glucose‐induced loss of podocin, since silencing Galectin‐1 in podocytes increased podocin expression in the presence of 25 mM glucose. Thus expression of Galectin‐1 in diabetic nephropathy may serve as a marker and contribute to disease progression by interfering with podocin expression.
New Iridium Catalysts for the Selective Alkylation of Amines by Alcohols under Mild Conditions and for the Synthesis of Quinolines by Acceptor‐less Dehydrogenative Condensation
Authors: Ruch Susanne , Irrgang Torsten , Kempe Rhett 
Published: September 2014

AbstractA novel family of iridium catalysts stabilised by P,N‐ligands have been introduced. The ligands are based on imidazo[1,5‐b]pyridazin‐7‐amines and can be synthesised with a broad variety of substitution patterns. The catalysts were synthesised quantitatively from the protonated ligands and a commercially available iridium precursor. The catalysts mediate the alkylation of amines by alcohols under mild conditions (70 °C). In addition, the synthesis of quinolines from secondary or primary alcohols and amino alcohols is reported. This sustainable synthesis proceeds through the liberation of two equivalents of water and two equivalents of dihydrogen. The investigations indicate that catalysts suitable for hydrogen autotransfer or borrowing hydrogen chemistry might also be suitable for acceptor‐less dehydrogenative condensation reactions.
Prussian Blues as a Cathode Material for Lithium Ion Batteries
Authors: Shen Lian , Wang Zhaoxiang , Chen Liquan 
Published: September 2014

AbstractPrussian blues (or iron cyanides) and their analogues are attractive in both fundamental studies and industrial applications owing to their chemical and structural diversity. The large open space in their framework provides tunnels and space for the transport and storage of lithium ions. Two Prussian blues were synthesized by a co‐precipitation method. The nanosized Fe4[Fe(CN)6]3 and cubic FeFe(CN)6 deliver reversible capacities of 95 mAh g−1 and 138 mAh g−1, respectively. In comparison, FeFe(CN)6 shows cycling and rate performances superior to Fe4[Fe(CN)6]3.
Voltage‐Driven Photoluminescence Modulation of Liquid‐Crystalline Hybridized ZnO Nanoparticles
Authors: Neaime Chrystelle , Prévôt Marianne , Amela‐Cortes Maria , Cîrcu Viorel , Grasset Fabien , Folliot Hervé , Molard Yann 
Published: September 2014

AbstractLiquid‐crystalline hybrid nanomaterials have been obtained by grafting mesogenic units around luminescent ZnO nanocrystals of 5 nm in diameter. Modifying the mesogenic density around the inorganic core allows the modulation of the liquid‐crystalline behavior and its miscibility in commercial liquid crystal (LC). The strong blue photoluminescence observed for the hybrids can be modulated by applying a voltage on a LC cell containing commercial LC and 10 wt % of hybrid.
Explosives Sensing by Using Electron‐Rich Supramolecular Polymers: Role of Intermolecular Hydrogen Bonding in Significant Enhancement of Sensitivity
Authors: Gole Bappaditya , Song Wentao , Lackinger Markus , Mukherjee Partha Sarathi 
Published: September 2014

AbstractWe demonstrate here that supramolecular interactions enhance the sensitivity towards detection of electron‐deficient nitro‐aromatic compounds (NACs) over discrete analogues. NACs are the most commonly used explosive ingredients and are common constituents of many unexploded landmines used during World War II. In this study, we have synthesised a series of pyrene‐based polycarboxylic acids along with their corresponding discrete esters. Due to the electron richness and the fluorescent behaviour of the pyrene moiety, all the compounds act as sensors for electron‐deficient NACs through a fluorescence quenching mechanism. A Stern–Volmer quenching constant determination revealed that the carboxylic acids are more sensitive than the corresponding esters towards NACs in solution. The high sensitivity of the acids was attributed to supramolecular polymer formation through hydrogen bonding in the case of the acids, and the enhancement mechanism is based on an exciton energy migration upon excitation along the hydrogen‐bond backbone. The presence of intermolecular hydrogen bonding in the acids in solution was established by solvent‐dependent fluorescence studies and dynamic light scattering (DLS) experiments. In addition, the importance of intermolecular hydrogen bonds in solid‐state sensing was further explored by scanning tunnelling microscopy (STM) experiments at the liquid–solid interface, in which structures of self‐assembled monolayer of the acids and the corresponding esters were compared. The sensitivity tests revealed that these supramolecular sensors can even detect picric acid and trinitrotoluene in solution at levels as low as parts per trillion (ppt), which is much below the recommended permissible level of these constituents in drinking water.
Efficient Synthesis of 1,2,3‐Triazoles by Copper‐Mediated CN and NN Bond Formation Starting From N‐Tosylhydrazones and Amines
Authors: Chen Zhengkai , Yan Qiangqiang , Yi Hong , Liu Zhanxiang , Lei Aiwen , Zhang Yuhong 
Published: September 2014

AbstractAn effective copper‐mediated synthesis of 1,5‐dialkyl‐4‐aryl‐1,2,3‐triazoles and 1,4‐dialkyl‐5‐aryl‐1,2,3‐triazoles has been achieved by the use of different N‐tosylhydrazones and alkyl amines. The scope of the substrates could be extended from anilines to aliphatic amines when 30 mol % amino acid is added into the reaction mixture. This methodology exhibits many notable features, such as broad substrates scope, high efficiency, and good regioselectivity. Preliminary mechanistic studies indicated that the reaction probably proceeded through a 1‐tosyl‐2‐vinyldiazene intermediate and subsequent aza‐Michael addition and NN bond formation process.
Cube‐in‐Cube Hollow Cu9S5 Nanostructures with Enhanced Photocatalytic Activities in Solar H2 Evolution
Authors: Xu Miao , Wang Min , Ye Tiannan , Liang Na , Jin Lun , Zai Jiantao , Qian Xuefeng 
Published: September 2014

AbstractHydrogen produced from water under solar energy is an ideal clean energy source, and the efficiency of hydrogen production usually depends on the catalytic systems based on new compounds and/or a unique nanostructure. Herein, well‐defined cube‐in‐cube hollow Cu9S5 nanostructures have been successfully prepared with Cu2O nanocubes and CS2 as precursors, and single‐shell hollow Cu9S5 nanocubes could be obtained by replacing CS2 with Na2S. The formation mechanism of cube‐in‐cube hollow nanostructures has been proposed based on the Kirkendell effect and an outward self‐assembly process. Further studies revealed that the cube‐in‐cube hollow Cu9S5 nanostructures exhibited better photocatalytic activity toward solar H2 evolution and would be a promising photocatalyst in the solar hydrogen industry.
(N‐Phosphinoamidinate)cobalt‐Catalyzed Hydroboration: Alkene Isomerization Affords Terminal Selectivity
Authors: Ruddy Adam J. , Sydora Orson L. , Small Brooke L. , Stradiotto Mark , Turculet Laura 
Published: September 2014

AbstractHerein we establish the utility of a three‐coordinate (N‐phosphinoamidinate)cobalt(amido) pre‐catalyst that is capable of effecting challenging alkene isomerization/hydroboration processes at room temperature, leading to the selective terminal addition of the boron group.
Financing constraints and US cross‐listing
Authors: Charest Guy , Cosset Jean‐Claude , Marhfor Ahmed , M'Zali Bouchra 
Published: September 2014

AbstractThis study investigates whether relaxation of firms' financial constraints is an important outcome of the US cross‐listing mechanism. We use the association between investment spending and cash flow to test for the presence and importance of firms' financing constraints. Consistent with the bonding hypothesis, the results suggest that US exchange and private placement cross‐listings significantly alleviate firms' financing constraints. In addition, the financial benefits associated with exchange listings are larger than those associated with private listings, while on the other hand, over‐the‐counter programs do not improve capital allocation. The study also shows that US exchange cross‐listing benefits have not been eroded by the enactment of the Sarbanes‐Oxley (SOX) Act in 2002. Copyright © 2014 ASAC. Published by John Wiley & Sons, Ltd.
The influence of top management team traits on corporate financial performance in the US
Authors: Dhaouadi Karima 
Published: September 2014

AbstractThis article aims to study the effect of human capital on the financial performance of the most admired American firms. Human capital is measured in terms of the demographic attributes and the diversity of top managers. The use of panel linear multiple regressions reveals that the most successful firms are characterized by coherent and homogeneous top management teams with moderate tenure and excellent wages. Moreover, the heterogeneity of managers is recommended for fairly young top management teams and for larger firms operating in a turbulent environment. Copyright © 2014 ASAC. Published by John Wiley & Sons, Ltd.
Validation of mammalian target of rapamycin biomarker panel in patients with clear cell renal cell carcinoma
Authors: Haddad Ahmed Q. , Kapur Payal , Singla Nirmish , Raman Jay D. , Then Matthew T. , Nuhn Philipp , Buchner Alexander , Bastian Patrick , Seitz Christian , Shariat Shahrokh F. , Bensalah Karim , Rioux‐Leclercq Nathalie , Sagalowsky Arthur , Lotan Yair , Margulis Vitaly 
Published: September 2014

BACKGROUNDThis was an external validation of the prognostic benefit of mammalian target of rapamycin (mTOR) marker panel in patients with clear cell renal cell carcinoma (ccRCC).METHODSImmunohistochemistry for 5 mTOR pathway markers was performed on tissue microarrays of patients with nonmetastatic ccRCC treated surgically at 4 centers. The markers employed were phosphatase and tensin homolog (PTEN), phosphoinositide 3‐kinase (PI3K), phosphorylated‐mTOR (p‐mTOR), phosphorylated‐S6 (p‐S6), and phosphorylated 4E‐binding protein‐1 (p‐4EBP1). Cox regression was used to correlate marker status and oncologic outcomes. Discrimination of the models was determined using area under the curve and net reclassification improvement.RESULTSFive hundred twenty‐eight patients with a median follow‐up of 56.5 months were included. Expression of PI3K, PTEN, p‐mTOR, p‐4EBP1, and p‐S6 was altered in 52%, 78%, 25%, 86%, and 30% of patients, respectively. The number of altered biomarkers predicted recurrence‐free survival (RFS) in multivariate analysis adjusted for stage, grade, and lymph node status (HR, 3.20; P = .02 for patients with 4‐5 altered biomarkers compared with 0‐1 altered markers). A biomarker panel consisting of only 2 markers (p‐S6 and p‐4EBP1) independently predicted for worse RFS (HR, 4.38; P = .003 for patients with 2 altered markers compared to patients with 0 altered markers). The biomarker score increased predictive accuracy when added to the clinical Cox regression model.CONCLUSIONSm‐TOR pathway biomarkers add prognostic information in addition to standard clinicopathologic variables in ccRCC patients and may identify patients who could benefit from additional treatments or closer postoperative surveillance. Cancer 2015;121:43–50. © 2014 American Cancer Society.