bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences in long-term fixation between mobile-bearing and fixed-bearing knee prostheses at ten to 12 years’ follow-up
Authors: Pijls B. G. , Valstar E. R. , Kaptein B. L. , Nelissen R. G. H. H. 
Published: September 2012

This single-blinded randomised controlled trial investigated whether one design of mobile-bearing (MB) total knee replacement (TKR) has any advantage over a fixed-bearing (FB) design on long-term fixation as measured by radiostereometry. The amount of wear underneath the mobile bearing was also evaluated. A series of 42 knees was randomised to MB or FB tibial components with appropriate polyethylene inserts and followed for between ten and 12 years, or until the death of the patient. The polyethylene in the MB group was superior in that it was gamma-irradiated in inert gas and was calcium-stearate free; the polyethylene in the FB group was gamma-irradiated in air and contained calcium stearate. In theory this should be advantageous to the wear rate of the MB group. At final follow-up the overall mean migration was 0.75 mm (sd 0.76) in the MB group and 0.66 mm (sd 0.4) in the FB group, with the FB group demonstrating more posterior tilt and the MB group more internal rotation. In the FB group there was one revision for aseptic loosening, but none in the MB group. There were no significant differences in clinical or radiological scores.For the MB group, the mean linear wear rate on the under-surface was 0.026 mm/year (sd 0.014). This was significantly smaller than the wear rate of 0.11 mm/year (sd 0.06) in the MB between femur and polyethylene (p < 0.001). Nevertheless, even in a best-case setting the mobile bearings of this TKR design had no apparent advantage in terms of fixation over the FB knee prosthesis at ten to 12 years. The wear underneath the mobile bearing was small and is unlikely to be clinically relevant.
Does obesity influence clinical outcome at nine years following total knee replacement?
Authors: Collins R. A. , Walmsley P. J. , Amin A. K. , Brenkel I. J. , Clayton R. A. E. 
Published: September 2012

A total of 445 consecutive primary total knee replacements (TKRs) were followed up prospectively at six and 18 months and three, six and nine years. Patients were divided into two groups: non-obese (body mass index (BMI) < 30 kg/m2) and obese (BMI ≥ 30 kg/m2). The obese group was subdivided into mildly obese (BMI 30 to 35 kg/m2) and highly obese (BMI ≥ 35 kg/m2) in order to determine the effects of increasing obesity on outcome. The clinical data analysed included the Knee Society score, peri-operative complications and implant survival. There was no difference in the overall complication rates or implant survival between the two groups.Obesity appears to have a small but significant adverse effect on clinical outcome, with highly obese patients showing lower function scores than non-obese patients. However, significant improvements in outcome are sustained in all groups nine years after TKR. Given the substantial, sustainable relief of symptoms after TKR and the low peri-operative complication and revision rates in these two groups, we have found no reason to limit access to TKR in obese patients.
Risk factors associated with surgical site infection in 30 491 primary total hip replacements
Authors: Namba R. S. , Inacio M. C. S. , Paxton E. W. 
Published: September 2012

We examined patient and surgical factors associated with deep surgical site infection (SSI) following total hip replacement (THR) in a large integrated healthcare system. A retrospective review of a cohort of primary THRs performed between 2001 and 2009 was conducted. Patient characteristics, surgical details, surgeon and hospital volumes, and SSIs were identified using the Kaiser Permanente Total Joint Replacement Registry (TJRR). Proportional-hazard regression models were used to assess risk factors for SSI. The study cohort consisted of 30 491 THRs, of which 17 474 (57%) were performed on women. The mean age of the patients in the whole series was 65.5 years (13 to 97; sd 11.8) and the mean body mass index was 29.3 kg/m2 (15 to 67; sd 5.9). The incidence of SSI was 0.51% (155 of 30 491). Patient factors associated with SSI included female gender, obesity, and American Society of Anesthesiologists (ASA) score ≥ 3. Age, diagnosis, diabetes and race were not associated with SSI. The only surgical factor associated with SSI was a bilateral procedure. Surgeon and hospital volumes, use of antibiotic-laden cement, fixation method, laminar flow, body exhaust suits, surgical approach and fellowship training were not associated with risk of SSI. A comprehensive infection surveillance system, combined with a TJRR, identified patient and surgical factors associated with SSI. Obesity and chronic medical conditions should be addressed prior to THR. The finding of increased SSI risk with bilateral THR requires further investigation.
Spondylodiscitis in infancy
Authors: Tsirikos A. I. , Tome-Bermejo F. 
Published: September 2012

An eight-week-old boy developed severe thoracic spondylodiscitis following pneumonia and septicaemia. A delay in diagnosis resulted in complete destruction of the T4 and T5 vertebral bodies and adjacent discs, with a paraspinal abscess extending into the mediastinum and epidural space. Antibiotic treatment controlled the infection and the abscess was aspirated. At the age of six months, he underwent posterior spinal fusion in situ to stabilise the spine and prevent progressive kyphosis. At the age of 13 months, repeat imaging showed lack of anterior vertebral body re-growth and he underwent anterior spinal fusion from T3 to T6 and augmentation of the posterior fusion. At the age of five years, he had no symptoms and radiographs showed bony fusion across the affected levels. Spondylodiscitis should be included in the differential diagnosis of infants who present with severe illness and atypical symptoms. Delayed diagnosis can result in major spinal complications with a potentially fatal outcome.
Hepaticocholecystoenterostomy as an alternative to hepaticojejunostomy for biliary bypass
Authors: Gani J , Lewis K 
Published: September 2012

INTRODUCTION Hepaticojejunostomy is the standard biliary bypass technique for periampullary cancer when trial dissection reveals unresectable disease or endoscopic stent placement is not possible. This anastomosis can be technically demanding and potentially difficult. The simpler technique of hepaticocholecystoenterostomy (HCE) has only previously been reported in very limited numbers and without outcome data. METHODS All patients undergoing HCE for the management of periampullary cancer were identified from a prospectively maintained computerised database of a single surgeon and were reviewed retrospectively. The HCE technique achieves a biliary bypass by two anastomoses, using the gallbladder as a conduit. It involves an anastomosis of the infundibulum of the gallbladder to the common hepatic duct followed by a second anastomosis of the gallbladder fundus to the proximal small bowel. RESULTS From 1996 to 2010, 30 patients with pancreatic adenocarcinoma required a biliary bypass after a failed trial of Whipple procedure (80%) or failed endoscopic stenting (20%). There were 19 men and 11 women with a mean age of 64.5 years. The mean operative time for HCE alone was 92 minutes. The mean length of hospital stay was nine days. There was a single grade 2 complication (readmission with gastric emptying delay) and a single grade 3 complication (bile leak requiring reoperation). Thirty-day mortality was zero and the mean survival was 12 months (with one patient still alive at the time of writing). There were no readmissions with recurrent biliary obstruction or cholangitis. One patient had developed an incisional hernia by the 24 month follow-up appointment. CONCLUSIONS HCE in periampullary cancer is safe and effective in selected patients. It involves two simple anastomoses with good access rather than one more demanding anastomosis. Morbidity, patency and overall survival are comparable with contemporary published series of hepaticojejunostomy.
“Know that I, Ringo the drummer, am”: Shakespeare, YouTube, and the Limits of Performance
Authors: Geddes Louise 
Published: September 2012

The Beatles' 1964 performance of the 'Pyramus and Thisbe' playlet from A Midsummer Night's Dream , originally an oddball product of variety television, has gained a new life as a popular clip on YouTube. This essay explores the video's engagement with online theatrical culture by way of a complex interplay of source and context. As performed by The Beatles, 'Pyramus and Thisbe' mocks classroom Shakespeare while simultaneously drawing on its reputation to position the band in a playful - and lucrative - space between high culture and counterculture. The sketch also locates The Beatles on multiple planes of performance and reality: their deliberately poor performances offer a glimpse of the 'real' Beatles while also recreating Shakespeare's rude mechanicals and highlighting the artificiality, not just of the play, but of 'The Beatles' as an image in itself. Finally, the sketch's presence on YouTube adds another layer of spectator/image recursion, as the online audience appropriates both Shakespeare and The Beatles, while unconsciously mimicking prior audiences of the 1960s and the 1590s.
Quantitative PCR Analysis of DNA, RNAs, and Proteins in the Same Single Cell
Authors: Ståhlberg Anders , Thomsen Christer , Ruff David , Åman Pierre 
Published: September 2012

BACKGROUNDThe single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes.METHODSReverse transcription and the proximity ligation assay were coupled with quantitative PCR and used to quantify any combination of DNA, mRNAs, microRNAs (miRNAs), noncoding RNAs (ncRNAs), and proteins from the same single cell. The method was used on transiently transfected human cells to determine the intracellular concentrations of plasmids, their transcribed mRNAs, translated proteins, and downstream RNA targets.RESULTSWe developed a whole-cell lysis buffer to release unfractionated DNA, RNA, and proteins that would not degrade any detectable analyte or inhibit the assay. The dynamic range, analytical sensitivity, and specificity for quantifying DNA, mRNAs, miRNAs, ncRNAs, and proteins were shown to be accurate down to the single-cell level. Correlation studies revealed that the intracellular concentrations of plasmids and their transcribed mRNAs were correlated only moderately with translated protein concentrations (Spearman correlation coefficient, 0.37 and 0.31, respectively; P &lt; 0.01). In addition, an ectopically expressed gene affected the correlations between analytes and this gene, which is related to gene regulation.CONCLUSIONSThis method is compatible with most cell-sampling approaches, and generates output for the same parameter for all measured analytes, a feature facilitating comparative data analysis. This approach should open up new avenues in molecular diagnostics for detailed correlation studies of multiple and different classes of analytes at the single-cell level.
Interaction of methocarbamol and yeast sucrase induces enzyme inhibition
Authors: Minai-Tehrani Dariush , Masoudnia Allaleh , Alavi Sana , Osmani Raheleh , Lotfi Leila , Asghari Mitra , Pirsalehi Mahdi , Sobhani-Damavandifar Zahra 
Published: September 2012

Abstract

Background: Methocarbamol is a skeletal muscle relaxant and is widely used to relieve pain in muscles. Many drugs may have interactions with each other when used at the same time. Yeast sucrase is taken as a drug by patients with congenital sucrase-isomaltase deficiency (CSID).

Methods: In this study, the interaction between methocarbamol and yeast sucrase was investigated.

Results: Our results showed that methocarbamol can inhibit sucrase activity and reduce the maximum reaction velocity (Vmax) of the enzyme by a non-competitive pattern. Measurement of IC50 and Ki of the drug revealed that methocarbamol did not bind the enzyme with high affinity. Fluorescence measurement showed that the drug binds to free enzyme and enzyme-substrate complexes that were accompanied by structural changes on the enzyme. Guaifenesin, which has a similar structure to methocarbamol, does not affect the activity of sucrase.

Conclusions: Methocarbamol inhibits sucrase activity and its carbamate group plays a main role in the binding of drug to sucrase.
Exosomes: the ideal nanovectors for biodelivery
Authors: Fais Stefano , Logozzi Mariantonia , Lugini Luana , Federici Cristina , Azzarito Tommaso , Zarovni Natasa , Chiesi Antonio 
Published: September 2012

Abstract
Nanomedicine aims to exploit the improved and often novel physical, chemical, and biological properties of materials at the nanometric scale, possibly with the highest level of biomimetism, an approach that simulates what occurs in nature. Although extracellularly released vesicles include both microvesicles (MVs) and exosomes, only exosomes have the size that may be considered suitable for potential use in nanomedicine. In fact, recent reports have shown that exosomes are able to interact with target cells within an organ or at a distance using different mechanisms. Much is yet to be understood about exosomes, and currently, we are looking at the visible top of an iceberg, with most of what we have to understand on these nanovesicles still under the sea. In fact, we know that exosomes released by normal cells always trigger positive effects, whereas those released by cells in pathological condition, such as tumor or infected cells, may induce undesired, dangerous, and mostly unknown effects, but we cannot exclude the possibility that exosomes may also be detrimental for the body in normal conditions. However, whether we consider extracellular vesicles as a whole, thus including MVs, it appears that even in normal conditions, extracellular vesicles may lead to unwanted effects, depending on gender and age. This review aims to critically emphasize existing data in the literature that support the possible roles of exosomes in both diagnostic and therapeutic scopes.
Acetylcholinesterase (AChE) inhibitory activity, antioxidant properties and phenolic composition of two Aframomum species
Authors: Adefegha Stephen A. , Oboh Ganiyu 
Published: September 2012

Abstract

Background:
Aframomum species are widely used as a food supplement and remedy in folklore medicine for the management of several diseases. This study was designed to investigate the acetylcholinesterase inhibitory activity and antioxidant properties of phenolic-rich extracts from two Aframomum species: Aframomum danielli (Hook F.) K. Schum (Zingiberaceae) and Aframomum melegueta (Roscoe) K. Schum (Zingiberaceae) seeds.

Methods: Acetylcholinesterase inhibitory activity and antioxidant properties [inhibition of quinolinic acid (QA)-induced lipid peroxidation in rat brain, reducing properties, 2,2-azinobis (3-ethylbenzo-thiazoline-6-sulfonate) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging abilities] using in vitro models were evaluated. Phenolic composition of the seed extracts using reversed phase high performance chromatography (RP-HPLC) and gas chromatography coupled with flame ionization detector (GC-FID) were also assessed.

Results: Both extracts exhibited acetylcholinesterase inhibitory activity in a dose-dependent manner (125–1000 μg/mL); however, A. melegueta extract (IC50=373.33 μg/mL) had a significantly higher (p<0.05) acetylcholinesterase inhibitory activity than A. danielli extract (IC50=417.10 μg/mL). Furthermore, both extracts significantly decreased QA-elevated brain malondialdehyde (MDA) contents, reduced Fe3+ to Fe2+ and scavenged DPPH and ABTS radicals. Phenolic characterization of the seeds by RP-HPLC at 280 nm showed abundance of quercetin and kaempferol in A. melegueta and chlorogenic acid in A. danielli, whereas GC-FID revealed that p-hydroxybenzoic acid was abundant in both seeds.

Conclusions: Inhibitory effect of these extracts on acetylcholinesterase activity and their antioxidant property could be attributed to the combined effect of phenolic and non-phenolic constituents of the seeds. These effects could be part of the possible biochemical mechanism by which these seeds elicit their protection against oxidative stress in brain; however, A. melegueta showed the more promising potential.
Mechanism of the experimental antithrombotic effect of some apple varieties involves enhanced endogenous thrombolytic activity
Authors: Morishita Mai , Naemura Aki , Tamura Yukinori , Yamaya Hideaki , Tsuda Yuko , Okada Yoshio , Okada Kiyotaka , Matsuo Osamu , Yamamoto Junichiro 
Published: September 2012

AbstractPrevention of arterial thrombotic diseases has high priority in developed countries. As inappropriate diet is known to enhance the risk for acute thrombotic events, a regular diet with experimentally proven antithrombotic effect might be a beneficial way of prevention. The present study is part of a series of investigations testing fruits and vegetables for antithrombotic activity. The Global Thrombosis Test was used to screen sixteen different apple varieties for antiplatelet and thrombolytic activities. Thein vitroeffective varieties were further investigated using the laser-induced thrombosis model in mice. In order to investigate the mechanism, hemostatometry and flow-mediated vasodilation test (FMV) were performed. Apple varieties were grouped into subclasses according to their antithrombotic activity. AP-2, AP-13, AP-14 and AP-15 showed significant antithrombotic effect bothin vitroandin vivo. AP-8 was antithromboticin vitrobut could not determinein vivobecause of the shortage of the sample. The antithrombotic effect was mainly due to activation of endogenous thrombolytic mechanism. The mechanism of such enhanced thrombolysis was investigated using a synthetic inhibitor highly specific to plasmin, polyclonal IgG to t-PA and u-PA, and testing the antithrombotic effect in t-PA knockout mice. Antithrombotic activity was prevented by the synthetic plasmin inhibitor and IgG against t-PA but not against u-PA. There was no antithrombotic activity in t-PA knockout mice. Intake of antithrombotic apple filtrate did not affect FMV and platelet reactivity. The antithrombotic effect was heat stable at 100 °C for 10 min. These results suggest that the mechanism of antithrombotic activity involves an increased t-PA release from the vascular endothelium and an inhibition of plasminogen activator inhibitor (PAI-1) release from activated platelets. The present findings justify including antithrombotic apple varieties in an antithrombotic diet.
Modulation of hearing related proteins in the brain and inner ear following repeated blast exposures
Authors: Arun Peethambaran , Valiyaveettil Manojkumar , Biggemann Lionel , Alamneh Yonas , Wei Yanling , Oguntayo Samuel , Wang Ying , Long Joseph B. , Nambiar Madhusoodana P. 
Published: September 2012

AbstractEmerging studies show that blast exposure causes traumatic brain injury (TBI) and auditory dysfunction without rupture of tympanic membrane, suggesting central auditory processing impairment after blast exposure. There is limited information on the mechanisms of blast-induced TBI and associated peripheral and central auditory processing impairments. We utilized a repetitive blast exposure mouse model to unravel the mechanisms of blast TBI and auditory impairment. C57BL/6J mice were exposed to three repeated blasts (20.6 psi) using a shock tube, and the cerebellum was subjected to proteomic analysis. The data showed that calretinin and parvalbumin, two major calcium buffering proteins, were significantly up-regulated after repeated blast exposures, and this was confirmed by Western blotting. Since these proteins are reportedly involved in auditory dysfunction, we examined the inner ear and found both calretinin and parvalbumin were up-regulated, suggesting that modulation of these proteins plays a role in blast-induced peripheral and central auditory processing impairments. Expression of cleaved caspase-3 was also up-regulated in both regions indicating ongoing cellular apoptosis, possibly due to altered calcium homeostasis. These results provide a molecular basis for changes in central and peripheral auditory processing involving abnormal calcium homeostasis resulting in hearing impairment after blast exposure.
Urinary neutrophil gelatinase-associated lipocalin (uNGAL) levels in patients with nonalcoholic fatty liver disease
Authors: Tekkeşin Nilgün , Taga Yavuz , İbrişim Duygu , Gündoğan Nilgün 
Published: September 2012

AbstractBackground/AimsNonalcoholic fatty liver disease (NAFLD), one of the most common forms of chronic liver disease, is closely associated with obesity and insulin resistance (IR). Adipokines secreted by adipose tissue have recently been implicated in initiating and perpetuating the chronic inflammatory state observed in obesity and NAFLD. Recent studies suggest neutrophil gelatinase-associated lipocalin (NGAL) plays a key role in the pathogenesis of IR. The aims of this study were to determine urinary NGAL levels in patients with ultrasonography proven NAFLD and to correlate these levels with the metabolic profile and fibrosis grade in this population.MethodologyOur cohort consisted of 65 consecutive patients undergoing abdominal ultrasonography for clinical suspicion of NAFLD. Patients were subsequently divided into two groups: no steatosis (n=20) and steatosis (n=45). The stage of fibrosis was measured using a four-point scale. Urinary NGAL was measured by a specific microparticle enzyme immunoassay. The degree of insulin resistance was determined by the homeostatic model assessment (HOMA). Glucose, insulin, lipid profile, and transaminases were also measured.ResultsUrinary NGAL levels correlated with body mass index, HOMA, fasting glucose, and insulin levels in patients with steatosis. Moreover, uNGAL levels were higher in patients with steatosis compared to those with no steatosis (49.8 ng/mL and 22.7 ng/mL, respectively) with a statistically significant difference (p<0.001). A stepwise increase in uNGAL levels from patients without fibrosis (21.7 ng/mL) to patients with cirrhosis (47.4 ng/mL) was noted (p<0.001).ConclusionsOur study demonstrates that in adult patients with NAFLD, uNGAL levels correlate with BMI, insulin resistance, and lipid profiles, and identifies a novel association between uNGAL levels and hepatocellular injury in these patients.