bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo optical assessment of cerebral and skeletal muscle microvascular response to phenylephrine
Authors: Mawdsley Laura , Eskandari Rasa , Kamar Farah , Rajaram Ajay , Yip Lawrence C. M. , Abayomi Naomi , Milkovich Stephanie , Carson Jeffrey J. L. , St. Lawrence Keith , Ellis Christopher G. , Diop Mamadou 
Published: August 2024

AbstractThis study aimed to investigate the simultaneous response of the cerebral and skeletal muscle microvasculature to the same phenylephrine (PE) boluses. A hybrid optical system that combines hyperspectral near‐infrared spectroscopy (hs‐NIRS) and diffuse correlation spectroscopy (DCS) was used to monitor changes in tissue oxygenation and perfusion. Data were collected from the head and hind limb of seven male Sprague–Dawley rats while administering intravenous (IV) injections of PE or saline to all animals. The response to saline was used as a control. Skeletal muscle oxygenation decreased significantly after PE injection, while a statistically underpowered decrease in perfusion was observed, followed by an increase beyond baseline. Vascular conductance also decreased in the muscle reflecting the drug's vasoconstrictive effects. Tissue oxygenation and perfusion increased in the brain in response to PE. Initially, there was a sharp increase in cerebral perfusion but no changes in cerebral vascular conductance. Subsequently, cerebral flow and vascular conductance decreased significantly below baseline, likely reflecting autoregulatory mechanisms to manage the excess flow. Further, fitting an exponential function to the secondary decrease in cerebral perfusion and increase in muscular blood flow revealed a quicker kinetic response in the brain to adjust blood flow. In the skeletal muscle, PE caused a transient decrease in blood volume due to vasoconstriction, which resulted in an overall decrease in hemoglobin content and tissue oxygen saturation. Since PE does not directly affect cerebral vessels, this peripheral vasoconstriction shunted blood into the brain, resulting in an initial increase in oxygenated hemoglobin and oxygen saturation.
The role of EUS in the diagnosis of early chronic pancreatitis
Authors: Bai Yaya , Qin Xianzheng , Ao Xiang , Ran Taojing , Zhou Chunhua , Zou Duowu 
Published: August 2024

ABSTRACT
The diagnosis of early chronic pancreatitis (ECP) is challenging due to the lack of standardized diagnostic criteria. EUS has been considered a sensitive diagnostic modality for chronic pancreatitis (CP), with advancements in technique such as EUS-guided fine needle aspiration and biopsy (EUS-FNA/FNB) being developed. However, their role in the diagnosis of ECP remains unelucidated. This review thereby aimed to provide an overview of the clinical landscape of EUS in the field of ECP.
GATA4 downregulation enhances CCL20-mediated immunosuppression in hepatocellular carcinoma
Authors: Nasir N. Jannah M. , Chuah Samuel , Shuen Timothy , Prawira Aldo , Ba Rebecca , Lim Mei Chee , Chua Joelle , Nguyen Phuong H. D. , Lim Chun J. , Wasser Martin , Hazirah Sharifah N. , Lim Tony K. H. , Leow Wei Qiang , Loh Tracy Jiezhen , Wan Wei Keat , Pang Yin Huei , Soon Gwyneth , Cheow Peng Chung , Kam Juinn Huar , Iyer Shridhar , Kow Alfred , Dan Yock Young , Bonney Glenn K. , Chung Alexander , Goh Brian K. P. , Chow Pierce K. H. , Albani Salvatore , Zhai Weiwei , Ouyang John F. , Toh Han Chong , Chew Valerie 
Published: August 2024

Background:
Hepatocellular carcinoma (HCC) is a deadly cancer with a high global mortality rate, and the downregulation of GATA binding protein 4 (GATA4) has been implicated in HCC progression. In this study, we investigated the role of GATA4 in shaping the immune landscape of HCC.

Methods:
HCC tumor samples were classified into “low” or “normal/high” based on GATA4 RNA expression relative to adjacent non-tumor liver tissues. The immune landscapes of GATA4-low and GATA4-normal/high tumors were analyzed using cytometry by time-of-flight, bulk/spatial transcriptomic analyses and validated by multiplex immunofluorescence.

Results:

GATA4-low tumors displayed enrichment in exhausted programmed cell death protein 1+ T cells, immunosuppressive regulatory T cells, myeloid-derived suppressor cells, and macrophages, highlighting the impact of GATA4 downregulation on immunosuppression. Spatial and bulk transcriptomic analyses revealed a negative correlation between GATA4 and C-C Motif Chemokine Ligand 20 (CCL20) expression in HCC. Overexpressing GATA4 confirmed CCL20 as a downstream target, contributing to an immunosuppressive tumor microenvironment, as evidenced by increased regulatory T cells and myeloid-derived suppressor cells in CCL20-high tumors. Lastly, the reduced expression of GATA4 and higher expression of CCL20 were associated with poorer overall survival in patients with HCC, implicating their roles in tumor progression.

Conclusions:
Our study reveals that GATA4 downregulation contributes to an immunosuppressive microenvironment, driven by CCL20-mediated enrichment of regulatory T cells and myeloid-derived suppressor cells in HCC. These findings underscore the critical role of GATA4 reduction in promoting immunosuppression and HCC progression.
Evaluation of Retinal Arterial Occlusion and its Visual and Systemic Prognosis after Hyperbaric Oxygen Therapy
Authors: Kalaw Fritz Gerald P. , Chartrand Nicholas , Wedekind Lauren , Chen Jimmy S. , Lin Andrew C. , Koretz Zachary , Meller Leo , Oca Michael , Jagadeesh Vasan , Wilson Katherine , Walker Evan , Freeman William R. , Toomey Christopher B. 
Published: August 2024

Purpose:
To evaluate the systemic and ocular outcomes of patients with branch retinal artery occlusion (BRAO) and central retinal artery occlusion (CRAO) after hyperbaric oxygen therapy (HBOT).

Methods:
This is a single-institution study of 75 subjects diagnosed with BRAO (28, 37.3%) and CRAO (47, 62.7%) who visited the emergency department or stroke clinic. Twenty-seven (36%) subjects received HBOT on initial presentation (BRAO–14.3%, CRAO–48.9%). The primary outcome was the best corrective visual acuity (BCVA) change in non-HBOT and HBOT subjects. Secondary outcomes included subsequent development of an acute cerebrovascular accident (CVA)/stroke or neovascular glaucoma (NVG).

Results:
Overall BCVA did not change from the initial presentation to the final timepoint (logMAR 1.5) in either the conservative management or HBOT cohorts for either BRAO subjects (non-HBOT–logMAR 0.4 vs. 0.6, p=0.658; HBOT–logMAR 0.1 vs. 0.4, p=0.207) or CRAO subjects (non-HBOT–logMAR 2.1 vs. 2.2, p=0.755; HBOT–logMAR 2.1 vs. 2.0, p=0.631). Seven (9.3%) subjects developed CVA (BRAO: non-HBOT–4.2% and HBOT–25.0%, p=0.207; CRAO: non-HBOT–16.7% and HBOT–4.3%, p=0.348) and five subjects (6.7%) developed NVG (BRAO: non-HBOT–4.2% and HBOT–0%, p=1.00; CRAO: non-HBOT–16.7% and HBOT–0%, p=0.109).

Conclusions:
Our findings suggest that HBOT does not significantly improve BCVA or mitigate the subsequent development of stroke and NVG in patients with RAOs.
Exploring the diagnostic and prognostic significance of circulating tumor cells in stage II-IV colorectal cancer using a nano-based detection method
Authors: Liu Gang , Zhu Jinfeng , Zhang Pengbo , Zhang Tingting , Cui Zheng , Jiao Fanglei , Le Wenjun , Li Xiaofeng , Chen Bingdi 
Published: August 2024

Background:
Colorectal cancer (CRC) is a leading cause of cancer mortality globally, underscoring the urgency for a noninvasive and effective biomarker to enhance patient prognosis. Circulating tumor cells (CTCs), a potential marker for real-time tumor monitoring, are limited in clinical utility due to the low sensitivity of existing detection methods. Previously, we introduced a novel nano-based CTCs detection method that relies on the electrical properties of cell surfaces, thus eliminating the need for specific molecular biomarkers. In this study, we used this technique to evaluate the diagnostic and prognostic value of CTCs in stage II-IV CRC.

Methods:
A total of 194 participants were included, consisting of 136 CRC patients and 58 healthy individuals. The peripheral blood of the participants was collected, and CTC enumeration was performed utilizing the nano-based detection method that we newly developed. The receiver operating characteristic (ROC) curve and multivariate Cox proportional-hazards analysis were used to assess the effectiveness of CTCs for diagnosing CRC and predicting patient prognosis.

Results:
The nano-based method demonstrated an ability to differentiate CRC patients from healthy individuals with a sensitivity of 84.6% and a specificity of 94.8%. Furthermore, baseline CTC levels were predictive of progression-free survival (PFS) in CRC patients, with lower levels associated with longer PFS compared to higher levels (4.5 vs 8.0 months at 15 CTCs/mL, p = 0.016; 4.4 vs 8.0 months at 20 CTCs/mL, p = 0.028). We also explored the dynamic changes in the number of CTCs after 1 to 5 cycles of chemotherapy. Patients with increasing CTC levels typically experienced disease progression (PD), while those with decreasing levels often achieved a partial response (PR) or maintained stable disease (SD). These findings suggest that the dynamic fluctuations in CTC counts are closely tied to the clinical course of the disease.

Conclusion:
Our study indicates the potential of nano-based CTCs detection in diagnosing and predicting outcomes for patients with stage II-IV CRC.
Implications for Socioenvironmental Determinants of Health-Informed Health Worker Wellness Programs
Authors: Kim Linda Y. , Martinez-Hollingsworth Adrienne , Coleman Bernice , Aronow Harriet U. 
Published: August 2024

Purpose
The aim of the study is to explore how socioenvironmental factors may potentially impact health outcomes across different groups of health workers (HWs).

Method
Data from the California Healthy Places Index (HPI) and administrative dataset including 12,715 HWs were analyzed using analysis of variance and two-step cluster analysis to evaluate variations in socioenvironmental factors across 11 HW job categories.

Results
While physicians had the highest average HPI percentiles (69th percentile) across almost all HPI components, the lower-wage HWs consistently ranked below the state average (50th percentile). Overall, the HPI percentile was lowest for environmental living conditions and pollution for all HCWs (30th percentile). The education (52nd percentile) and economic components (59th percentile) were higher than the state average.

Conclusions
The findings from this highlight the importance of socioenvironmental determinants of health-informed employee wellness programs and research studies.
Proceedings From a National Summit on Workplace Mental Health and Well-being
Authors: Roemer Enid Chung , Goetzel Ron Z. , Davis Meghan F. , Zhang Ying , Kent Karen B. , Harter Jim , McGee Ebony O. , Troester Joan M. , Hilton Lara , Stratton Kelcey J. , Vietas Jay , MacKenzie Ellen J. 
Published: August 2024

Objective
The aim of the study is to spotlight the challenges, gaps, and opportunities to improve workforce mental health and well-being in higher education institutions.

Methods
We convened a full-day summit of subject matter experts from academia, business, government, and practice to share research and best practices on workplace mental health.

Results
Highlights from the summit are presented in this paper covering the importance of leadership and culture; the mental health costs associated with being a Black STEM scholar; the role of the environment; case studies of three university mental health and well-being programs; and the future of work.

Conclusions
Establishing a culture of caring requires leadership commitment, strategic planning, accountability and shared responsibility, and measurement and evaluation. Higher education institution leaders are called to lead by example; foster community partnerships; adopt a Total Worker Health framework; and regularly evaluate progress.
The microbiota comparative analysis of the characteristics between colorectal adenomatous polyps and normal mucosal intestinal
Authors: Liu Ya , Lin Xiao-xiao , Hu Si-si , Zheng En-dian , Ye Yi , Xu Bei-bei , Wu Le-can 
Published: August 2024

Objective
The aim of this study is to systematically examine and compare the characteristics distinguishing colorectal adenomatous polyps from normal mucosal intestinal microbiota.

Methods
A total of 30 specimens were obtained from patients diagnosed with colorectal adenomatous polyps (adenoma group) who underwent endoscopic removal at Wenzhou People’s Hospital between September 2021 and November 2021. Concurrently, 30 normal mucosal specimens were collected from patients without adenomatous polyps (control group). Subsequently, microbiome total DNA extraction was carried out, followed by PCR amplification targeting the V3–V4 region of the 16S rDNA. High-throughput sequencing was conducted using the Illumina MiSeq platform. Subsequent to sequencing, bioinformatics analysis was used to assess the diversity, composition, and functional aspects of the intestinal microbiota in both study groups.

Results
A notable dissimilarity in the microbiota structure was identified, specifically within the transverse colon, between these two groups (P < 0.05). Species composition analysis revealed that Escherichia, Fusobacterium, and Bacteroides were predominant bacteria in both groups, with Escherichia and Enterobacter displaying significant differences at the genera level between the control group and the adenoma group (P < 0.05). Correlation analysis and functional prediction demonstrated substantial disparities in interactions among dominant intestinal microbial genera within patients from both groups. Additionally, it was discovered that the intestinal microbiomes in patients in the adenoma group exhibited a significantly higher pathogenic potential.

Conclusion
Upon conducting a comprehensive analysis, it was discerned that the microbiota present in the transverse colon of the control group exhibited distinctive characteristics that may contribute to the maintenance of intestinal health.
Incidence and Risk Factors for Genitourinary Tract Injury Within 1 Year After Surgery for Pelvic Organ Prolapse
Authors: Sheyn David , Gupta Ankita , Boyd Sara , Wu Xian , Shoag Jonathan , Bretschneider C. Emi 
Published: August 2024

Importance
The rate of genitourinary tract injury (GUTI) following pelvic organ prolapse (POP) surgery is presently ill-defined and based on relatively small trials with short follow-up time. Given the potential for higher risk of injury with POP, a better understanding of this type of injury is important for patient counseling.

Objectives
The objective of this study was to identify the incidence and risk factors of GUTI related to POP surgery.

Study Design
Women undergoing POP surgery between 2010 and 2019 were identified using Current Procedural Terminology codes in the Premier Healthcare Database. The primary outcome was GUTI, defined as bladder or ureteral injury, and vesicovaginal or ureterovaginal fistula within 1 year of surgery. Genitourinary tract injury was identified using International Classification of Diseases and Current Procedural Terminology codes. Patients were divided into those with and without GUTI. Differences between groups were evaluated using the Student t test, Wilcoxon rank-sum test, and Fisher exact test as appropriate. Multivariable logistic regression was used to evaluate the independent predictors of GUTI.

Results
One hundred thirty-nine thousand one hundred fifty-eight surgical procedures for POP were captured between 2010 and 2019. The rate of GUTI was 1.10%: 0.48% bladder, 0.64% ureteral injuries, and 0.01% fistulas. The most significant variables associated with any GUTI were as follows: adhesiolysis (adjusted odds ratio [aOR], 2.64; 95% confidence interval [CI], 1.07–6.51), blood transfusion (aOR, 3.67; 95% CI, 1.34–10.04), and low-volume surgeons (<12 cases per year) (aOR, 1.68; 95% CI, 1.60–1.77), nonurologic or gynecologic surgeon specialty (aOR, 1.62; 95% CI, 1.49–2.00), and uterosacral suspension (aOR, 1.30; 95% CI, 1.13–1.49).

Conclusions
The rate of GUTI following POP surgery is lower than has previously been reported. Surgeon experience and specialty and surgical approach may affect GUTI incidence.
AUGS-PERFORM: A New Patient-Reported Outcome Measure to Assess Quality of Prolapse Care
Authors: O’Shea Michele , Boyles Sarah , Bradley Catherine S. , Jacobs Kristin , McFatrich Molly , Sung Vivian , Weinfurt Kevin , Siddiqui Nazema Y. 
Published: August 2024

Objective
Patient-reported outcomes (PRO) are important for measuring quality of care, particularly for interventions aimed at improving symptom bother such as procedures for pelvic organ prolapse. We aimed to create a concise yet comprehensive PRO measurement tool to assess pelvic organ prolapse care in high-volume clinical environments.

Methods
The relevant concepts to measure prolapse treatment quality were first established through literature review, qualitative interviews, and a patient and provider-driven consensus-building process. Extant items mapping to these concepts, or domains, were identified from an existing pool of patient-reported symptoms and condition-specific and generic health-related quality of life measures. Item classification was performed to group items assessing similar concepts while eliminating items that were redundant, inconsistent with domains, or overly complex. A consensus meeting was held in March 2020 where patient and provider working groups ranked the remaining candidate items in order of relevance to measure prolapse treatment quality. After subsequent expert review, the revised candidate items underwent cognitive interview testing and were further refined.

Results
Fifteen relevant PRO instruments were initially identified, and 358 items were considered for inclusion. After 2 iterative consensus reviews and 4 rounds of cognitive interviewing with 19 patients, 11 final candidate items were identified. These items map 5 consensus-based domains that include awareness and bother from prolapse, physical function, physical discomfort during sexual activity, pain, and urinary/defecatory symptoms.

Conclusions
We present a concise set of candidate items that were developed using rigorous patient-centered methodology and a national consensus process, including urogynecologic patients and providers.
MagnoliidsGDB: An integrated functional genomics database for Magnoliids
Authors: Chen Yayu , Yang Zhuang , Chen Jiajie , Li Ping , Zhao Xinglong , Huang Sihui , Li Zhumao , Huang Sishu , Luo Jie , Hu Haiyan , Ding Yuanhao 
Published: August 2024

AbstractIn this study, we present MagnoliidsGDB, an integrated functional genomics database specifically developed for magnoliids, a group of plants known for their unique characteristics and medicinal properties. As of March 2024, MagnoliidsGDB has collected 31 genome sequences from 24 species, 149 resequencing datastes from 4 species, 842 transcriptomic datasets from 20 species, and metabolomic data from 15 species. This comprehensive resource allows researchers to explore the genomic, transcriptomic, and metabolomic variations of magnoliids. The user-friendly search and analysis functionalities of MagnoliidsGDB facilitate the study of genomic variations, gene functions, plant evolution and key metabolites of magnoliids. We hope this database will contribute to the study of magnoliids and increase our understanding of the origin, variation, and molecular basis of the unique biological features of magnoliids.
Effect of a novel food rich in miraculin on the intestinal microbiome of malnourished patients with cancer and dysgeusia
Authors: Plaza-Diaz Julio , Brandimonte-Hernández Marco , López-Plaza Bricia , Ruiz-Ojeda Francisco Javier , Álvarez-Mercado Ana Isabel , Arcos-Castellanos Lucía , Feliú-Batlle Jaime , Hummel Thomas , Palma Milla Samara , Gil Angel 
Published: August 2024

AbstractDysgeusia contributes to nutritional derangement and worsens the quality of life of patients with cancer. Despite the different strategies, there is no effective treatment for patients suffering from taste disorders provided by the pharmaceutical industry. We developed a novel strategy for reducing side effects in cancer patients by providing a novel food supplement with the tastemodifying glycoprotein miraculin, which is approved by the European Union, as an adjuvant to medical-nutritional therapy. A pilot randomized, parallel, triple-blind, and placebo-controlled intervention clinical trial was carried out in which 31 malnourished patients with cancer and dysgeusia receiving antineoplastic treatment, and were randomized into three arms: standard dose of DMB (150 mg DMB/tablet), high dose of DMB (300 mg DMB/tablet) or placebo (300 mg freeze-dried strawberry) for three months. Patients consumed a DMB or placebo tablet before each main meal (breakfast, lunch and dinner). Using stool samples from patients with cancer, we analyzed the intestinal microbiome via nanopore methodology. We detected differences in the relative abundances of generaPhocaeicolaandEscherichiadepending on the treatment. Nevertheless, only theSolibaculumgenus was more abundant in the standard-dose DMB group after 3 months. At the species level,Bacteroidessp. PHL 2737 presented a relatively low abundance in both DMB groups, andVescimonas coprocolapresented a relatively high abundance in both treatment groups after 3 months. Furthermore, a standard dose of DMB was positively associated with TNF-α levels andLachnoclostridiumandMediterraneibacterabundances, whereas a high dose of DMB was negatively associated with TNF-α levels and the relative abundance ofPhocaeicola. After a high dose of DMB, erythrocyte polyunsaturated fatty acids were positively correlated withLachnoclostridiumandRoseburia, and there was a positive association betweenPhocaeicolaand the acetic acid concentration of feces. The intake of DMB together with nutritional treatment and individualized dietary advice results in positive changes in the intestinal microbiome of patients with cancer and dysgeusia There was a negative association between the relative abundance ofPhocaeicolaand taste perception in the DMB high dose group. Changes observed in the intestinal microbiota might contribute to maintaining an appropriate immune response of cancer patients. Since the present pilot study involved only a few participants, further research is needed to draw robust conclusions.
Evoked resonant neural activity outperforms spectral markers in decoding sleep from the subthalamic nucleus
Authors: Wiest Christoph , Simpson Thomas G. , Pogosyan Alek , Hasegawa Harutomo , He Shenghong , Plazas Fernando Rodriguez , Wehmeyer Laura , Yassine Sahar , Guo Xuanjun , Shah Rahul , Merla Anca , Perera Andrea , Raslan Ahmed , O’Keeffe Andrew , Hart Michael G. , Morgante Francesca , Pereira Erlick A. , Ashkan Keyoumars , Tan Huiling 
Published: August 2024

ABSTRACTBackgroundDeep brain stimulation is a treatment for advanced Parkinson’s disease and currently tuned to target motor symptoms during daytime. Parkinson’s disease is associated with multiple nocturnal symptoms such as akinesia, insomnia and sleep fragmentation which may require adjustments of stimulation during sleep for best treatment outcome.ObjectivesThere is a need for a robust biomarker to guide stimulation titration across sleep stages. This study aimed to investigate whether evoked resonant neural activity (ERNA) is modulated during sleep.MethodsWe recorded local field potentials from the subthalamic nucleus of four Parkinson’s patients with externalised electrodes while applying single stimulation pulses to investigate the effect of sleep on ERNA.ResultsWe found that ERNA features change with wakefulness and sleep stages, and are correlated with canonical frequency bands and heart rate. We further evaluated the performance of machine learning models in classifying non-REM sleep versus wakefulness and found that ERNA amplitude outperforms all spectral markers.ConclusionsGiven the heterogeneity of spectral features during sleep, their susceptibility to movement artefacts and superior classification accuracy of models using ERNA features, this study paves the way for ERNA as a marker for automatic stimulation titration during sleep and improved patient care.