bluuebunny/crossref_metadata_2025_split huggingface dataset:
A hierarchical structure in the N-glycosylation process governs the N-glycosylation output: prolonged cultivation induces glycoenzymes expression variations that are reflected in the cellular N-glycome but not in the protein and site-specific glycoprofile of CHO cells
Authors: Arigoni-Affolter Ilaria , Losfeld Marie-Estelle , Hennig René , Rapp Erdmann , Aebi Markus 
Published: June 2024

Abstract
N-glycosylation is a central component in the modification of secretory proteins. One characteristic of this process is a heterogeneous output. The heterogeneity is the result of both structural constraints of the glycoprotein as well as the composition of the cellular glycosylation machinery. Empirical data addressing correlations between glycosylation output and glycosylation machinery composition are seldom due to the low abundance of glycoenzymes. We assessed how differences in the glycoenzyme expression affected the N-glycosylation output at a cellular as well as at a protein-specific level. Our results showed that cellular N-glycome changes could be correlated with the variation of glycoenzyme expression, whereas at the protein level differential responses to glycoenzymes alterations were observed. We therefore identified a hierarchical structure in the N-glycosylation process: the enzyme levels in this complex pathway determine its capacity (reflected in the N-glycome), while protein-specific parameters determine the glycosite-specificity. What emerges is a highly variable and adaptable protein modification system that represents a hallmark of eukaryotic cells.
Caregivers’ experience of caring for family members with complex health needs in Latvia
Authors: Petrova Indra , Pipere Anita 
Published: June 2024

Abstract
The article examines the experiences of family caregivers engaged in the provision of long-term care for their relatives with complex health needs within the Latvian context. Semi-structured interviews were conducted with seven caregivers who provide care in cases of dementia, depression, schizophrenia, opioid use, Down syndrome and mild cognitive impairment. A thematic analysis of interview transcripts revealed common themes, such as the initial experiences when encountering a family member’s disorder and the subsequent reactions, as well as the quest for support and resources. Variations mainly centered around differences in formal aspects and childhood experiences of care. Caregivers reported risk factors such as guilt, lack of support from family and friends, financial difficulties, deficiency of professional care and ignoring the caregiver’s own needs. Awareness of personal resources, values and limits, coping with personal stigma and improved overall quality of life were identified as protective factors. The findings underscore several preventive measures that social and mental health services could implement to mitigate the adverse effects of caregiving on caregivers’ lives.
Using Holocaust Education as a “Bridge” to Learning about Apartheid in a South African History Classroom: The Development of “Interpretive Frames” through Comparative Histories
Authors: Robinson Natasha 
Published: June 2024

Abstract
The Grade 9 South African history curriculum teaches the history of the Holocaust as an introduction to the study of apartheid. Students are taught how racial identities are constructed, codified into law, and weaponized for the purposes of discrimination and genocide. They are taught that racialized discrimination can happen anywhere—not only in South Africa—and that we must be vigilant lest it happens again. Curriculum developers—supported by international literature (e.g., UNESCO publications)—believe that learning about the Holocaust will act as a “bridge” to learning about apartheid.1 By teaching young South Africans difficult history that is emotionally, geographically, and temporally removed from their own country, educators believed that students will be better prepared to study the complex history of apartheid. Yet what is the impact of this comparative approach?2 The author’s ten-month ethnographic study explores how Grade 9 students in one majority-White history classroom in Cape Town learned about the Holocaust and apartheid. Developing Epstein’s idea of an “interpretive frame,”3 this empirical study contributes to ongoing debates on whether and how genocides and other atrocities can be meaningfully compared in the history classroom.4 Furthermore, it questions whether using Holocaust education as a “bridge”5 to domestic injustices and persecution will significantly improve historical understanding, tolerance, and moral reasoning.
Revisiting trophectoderm-inner cell mass lineage segregation in the mammalian preimplantation embryo
Authors: Skory Robin M 
Published: June 2024

Abstract
In the first days of life, cells of the mammalian embryo segregate into two distinct lineages, trophectoderm and inner cell mass. Unlike nonmammalian species, mammalian development does not proceed from predetermined factors in the oocyte. Rather, asymmetries arise de novo in the early embryo incorporating cues from cell position, contractility, polarity, and cell–cell contacts. Molecular heterogeneities, including transcripts and non-coding RNAs, have now been characterized as early as the 2-cell stage. However, it’s debated whether these early heterogeneities bias cells toward one fate or the other or whether lineage identity arises stochastically at the 16-cell stage. This review summarizes what is known about early blastomere asymmetries and our understanding of lineage allocation in the context of historical models. Preimplantation development is reviewed coupled with what is known about changes in morphology, contractility, and transcription factor networks. The addition of single-cell atlases of human embryos has begun to reveal key differences between human and mouse, including the timing of events and core transcription factors. Furthermore, the recent generation of blastoid models will provide valuable tools to test and understand fate determinants. Lastly, new techniques are reviewed, which may better synthesize existing knowledge with emerging data sets and reconcile models with the regulative capacity unique to the mammalian embryo.
The Ripple Effects of the Illegitimacy of War
Authors: O'Mahoney Joseph 
Published: June 2024

Abstracts
Recent data show systematic changes in the diplomacy and practice of war. Conquests, peace treaties, declarations of war, and state boundary changes have declined or disappeared. There are still wars, but they are increasingly fait accomplis, and their outcomes are often not recognized as legal. How can we explain this wide-ranging but seemingly contradictory transformation? Existing accounts, such as those based on a territorial integrity norm, do not adequately explain these changes. This paper uses norm dynamics theory to show that all of these changes can be explained as ‘ripple effects’ of war becoming illegitimate as a way to solve international disputes. The kinds of rhetorical justifications states can convincingly give for engaging in violence have changed. States are navigating this changed international social environment through legitimacy management behaviors. The paper specifies three types of ripple effect, Reframing, Displacement, and Consistency-Maintenance, corresponding to changes in what states say, the actions they perform, and how the audience reacts. We show how this theory unifies all of the existing data into a single explanatory framework. We also apply the theory to the decline of peace treaties to show how ripple effects play out in more detail.
Endophytic diazotrophic communities from rice roots are diverse and weakly associated with soil diazotrophic community composition and soil properties
Authors: Ferrando Lucía , Rariz Gastón , Martínez-Pereyra Andrea , Fernández-Scavino Ana 
Published: June 2024

Abstract

Aim
Bacteria that promote plant growth, such as diazotrophs, are valuable tools for achieving a more sustainable production of important non-legume crops like rice. Different strategies have been used to discover new bacteria capable of promoting plant growth. This work evaluated the contribution of soil diazotrophs to the endophytic communities established in the roots of rice seedlings cultivated on seven representative soils from Uruguay.

Methods and results
The soils were classified into two groups according to the C and clay content. qPCR, terminal restriction fragment length polymorphism (T-RFLP), and 454-pyrosequencing of the nifH gene were used for analyzing diazotrophs in soil and plantlets’ roots grown from seeds of the same genotype for 25 days under controlled conditions. A similar nifH abundance was found among the seven soils, roots, or leaves. The distribution of diazotrophs was more uneven in roots than in soils, with dominance indices significantly higher than in soils (nifH T-RFLP). Dominant soils’ diazotrophs were mainly affiliated to Alphaproteobacteria and Planctomycetota. Conversely, Alpha, Beta, Gammaproteobacteria, and Bacillota were predominant in different roots, though undetectable in soils. Almost no nifH sequences were shared between soils and roots.

Conclusions
Root endophytic diazotrophs comprised a broader taxonomic range of microorganisms than diazotrophs found in soils from which the plantlets were grown and showed strong colonization patterns.
Synthetic microbial ecology: engineering habitats for modular consortia
Authors: Chen Yue Casey , Destouches Louie , Cook Alice , Fedorec Alex J H 
Published: June 2024

Abstract
Microbiomes, the complex networks of micro-organisms and the molecules through which they interact, play a crucial role in health and ecology. Over at least the past two decades, engineering biology has made significant progress, impacting the bio-based industry, health, and environmental sectors; but has only recently begun to explore the engineering of microbial ecosystems. The creation of synthetic microbial communities presents opportunities to help us understand the dynamics of wild ecosystems, learn how to manipulate and interact with existing microbiomes for therapeutic and other purposes, and to create entirely new microbial communities capable of undertaking tasks for industrial biology. Here, we describe how synthetic ecosystems can be constructed and controlled, focusing on how the available methods and interaction mechanisms facilitate the regulation of community composition and output. While experimental decisions are dictated by intended applications, the vast number of tools available suggests great opportunity for researchers to develop a diverse array of novel microbial ecosystems.
Potential to take root in viticulture? An evaluation of mycorrhizal inoculants on the growth and nutrient uptake of young wine grapes planted in live field soil
Authors: Lueck Madeline R , Moyer Michelle M , Cheeke Tanya E 
Published: June 2024

Abstract

Aims
Incorporating biofertilizers, such as arbuscular mycorrhizal fungal (AM) fungal inoculants, into vineyard management practices may enhance vine growth and reduce environmental impact. Here, we evaluate the effects of commercially available and local AM fungal inoculants on the growth, root colonization, and nutrient uptake of wine grapes (Vitis vinifera) when planted in a field soil substrate.

Methods and results
In a greenhouse experiment, young wine grapes were planted in a field soil substrate and inoculated with one of three commercially available mycorrhizal inoculant products, or one of two locally collected whole soil inoculants. After 4 months of growth, inoculated vines showed no differences in plant biomass, colonization of roots by AM fungi, or foliar macronutrient concentrations compared to uninoculated field soil substrate. However, vines grown with local inoculants had greater shoot biomass than vines grown with mycorrhizal inoculant products.

Conclusions
Although effects from inoculations with AM fungi varied by inoculant type and source, inoculations may not improve young vine performance in field soils with a resident microbial community.
A One-Step Green Microwell Spectrophotometric Assay for the Determination of Certain New Chemotherapeutic Drug Formulations
Authors: Darwish Ibrahim A , Darwish Hany W , Alsalhi Mohammed S 
Published: June 2024

Abstract

Background
The formation of charge-transfer complexes (CTCs) of iodine with five chemotherapeutic drugs used for the treatment of different types of cancer has not been investigated. These drugs are olaparib, seliciclib, vandetanib, dasatinib, and tozasertib. Additionally, these drugs need an appropriate general spectrophotometric assay for their analysis in the dosage forms regardless of the differences in their chemical structures.

Objective
The aim of this study was the development of a novel microwell spectrophotometric assay (MW-SPA) for one-step determination of these drugs via their interactions with iodine, which resulted in instantaneous production of bright lemon-yellow CTCs.

Methods
A spectrophotometric study of the CTCs was conducted, and all CTCs were characterized. Site(s) of interaction on each drug were assigned, and the MW-SPA was developed and applied to the analysis of dosage forms.

Results
The findings confirmed that the reactions proceeded via CTC formation. Beer's law was obeyed over a general concentration range of 1–6 µg/mL. The LODs and LOQs were in the ranges of 0.5–2.1 and 1.5–6.4 µg/mL, respectively. The proposed MW-SPA demonstrated excellent precisions as the relative standard deviations were &lt; 2.24 and 2.23% for the intra- and inter-assay precision, respectively. Recovery studies demonstrated the accuracy of MW-SPA. Successful determination of all drugs in bulk and tablet forms was achieved using the MW-SPA. The environmental sustainability of the proposed methodology was determined, providing evidence of the assay's alignment with the basis of green analytical chemistry. The high throughput of the assay was documented.

Conclusion
In contrast to other existing methods, the MW-SPA described herein was valid for analyzing all drugs at the same wavelength.

Highlights
The assay is useful for routine analysis of drugs in their formulations in QC laboratories.
Effects of pyriproxyfen on development and hormone of the aphis, Aphis craccivora (Hemiptera: Aphididae)
Authors: Li Haolin , Zhang Wenjie , Zhang Yongheng , Guo Xiaxia , Hou Jiangan , Li Honghong , Wei Jiguang , Li Xuesheng 
Published: June 2024

Abstract
Pyriproxyfen (PPF) has been shown to affect the pupal stage and ecdysone levels in holometabolous insects, such as silkworms and mealworms. It remains unknown whether it affects hemimetabolous insects with their hormone levels in insects lacking a pupal stage. In this laboratory study, bioassays were conducted to investigate the effects of varying doses of PPF on Aphis craccivora Koch (Hemiptera: Aphididae). Ultraperformance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was used to determine the types and titers of juvenile hormone (JH) and 20-hydroxyecdysone (20E). Additionally, the effects of PPF on A. craccivora reproduction and molting, as well as its influence on relevant gene expression, were examined. The results revealed LC50 and LC90 values of 3.84 and 7.49 mg/l for PPF, respectively, after 48 h of exposure. The results demonstrated a significant reduction in the titer of JH III and a significant increase in the titer of 20E following treatment with PPF. However, there was no significant decrease observed in the titer of JH III skipped bisepoxide (JH SB3). A sublethal concentration of PPF was found to inhibit Krüppel homolog 1 (kr-h1) gene expression and reduce aphid reproduction, but it did not significantly impact ecdysone receptor expression and aphid molting. The results of this study demonstrate that PPF exhibits a lethal effect on aphids, thereby providing an effective means of control. Additionally, sublethal concentrations of PPF have been found to inhibit the JH in aphids, resulting in a decline in their reproductive ability and achieving the desired control objectives.
SUMO-specific protease 1 exacerbates acute myeloid leukemia by enhancing beclin 1-dependent autophagy through polypyrimidine tract-binding protein 1 deSUMOylation
Authors: Xing Lina , Guo Xuefei , Zhang Xiaolei , Wang Ying , Ren Jinhai 
Published: June 2024

Abstract
Genetic association between SUMO-specific protease 1 (SENP1) and acute myeloid leukemia (AML) has been validated. However, the mechanism by which SENP1 affects AML proliferation, apoptosis, and autophagy remains unknown. The levels of SENP1 and polypyrimidine tract-binding protein 1 (PTBP1) were measured in patients with AML, AML cell lines, and xenograft tissues. The effects of SENP1 on AML proliferation, apoptosis, and beclin 1 (BECN1)-dependent autophagy were assessed through in vitro and in vivo loss- or gain-of-function experiments. SUMOylation analysis using immunoprecipitation (IP), RNA pull-down, RNA IP (RIP), and RNA stability assays were used to explore the molecular mechanism of SENP1 in AML development. The SENP1 level was elevated in AML samples. Silencing SENP1 impeded the development of AML, as evidenced by the inhibition of proliferation and promotion of G1-phase arrest and apoptosis resulting from SENP1 depletion in AML cells. Moreover, silencing of SENP1 restrained BECN1-depentent autophagy in AML cells. In addition, the overexpression of BECN1 or PTBP1 partially neutralized the effect of SENP1 knockdown on AML cell behavior. Mechanistically, SENP1 mediated PTBP1 deSUMOylation, which then directly interacted with BECN1 mRNA and enhanced its stability. In vivo experiments further confirmed the repressive effects of SENP1 suppression on AML development. Collectively, the SENP1/PTBP1/BECN1 signaling axis has been identified as a significant therapeutic target for enhancing AML treatment.
Evaluation of PTV margin in CBCT-based online adaptive radiation therapy for gastric mucosa-associated lymphoid tissue lymphoma
Authors: Hirose Taka-aki , Takaki Masanori , Shibayama Yusuke , Fukunaga Jun-ichi , Kato Toyoyuki , Yoshitake Tadamasa , Ishigami Kousei 
Published: June 2024

Abstract
The aim of this study was to investigate planning target volume (PTV) margin in online adaptive radiation therapy (oART) for gastric mucosa-associated lymphoid tissue (MALT) lymphomas. Four consecutive patients with gastric MALT lymphoma ﻿who received oART (30 Gy in 15 fractions) on the oART system were included in this study. One hundred and twenty cone-beam computed tomography (CBCT) scans acquired pre- and post-treatment of 60 fractions for all patients were used to evaluate intra- and interfractional motions. Patients were instructed on breath-holding at exhalation during image acquisition. To assess the intrafraction gastric motion, different PTVs were created by isotropically extending the CTV contoured on a pre-CBCT image (CTVpre) at1 mm intervals. Intrafraction motion was defined as the amount of expansion covering the contoured CTV on post-CBCT images (CTVpost). Interfractional motion was defined as the amount of reference CTV expansion that could cover each CTVpre, as well as the evaluation of the intrafractional motion. PTV margins were estimated from the cumulative proportion of fraction covering the intra- and interfractional motions. The extent of expansion covering the CTVs in 90% of fractions was adopted as the PTV margin. The PTV margin for intrafractional gastric motion using the oART system with breath-holding was 14 mm. In contrast, the PTV margin for interfractional gastric organ motion without the oART system was 25 mm. These results indicated that the oART system can reduce the PTV margin by &gt;10 mm. Our results could be valuable data for oART cases.
Natural flavonoid glycosides Chrysosplenosides I & A rejuvenate intestinal stem cell aging via activation of PPARγ signaling
Authors: Ye Jinbao , Yan La , Yuan Yu , Fu Fang , Yuan Lu , Fan Xinxin , Zhou Juanyu , Zhu Yuedan , Liu Xingzhu , Ren Gang , Chen Haiyang 
Published: June 2024

Abstract
The decline in intestinal stem cell (ISC) function is a hallmark of aging, contributing to compromised intestinal regeneration and increased incidence of age-associated diseases. Novel therapeutic agents that can rejuvenate aged ISCs are of paramount importance for extending healthspan. Here, we report on the discovery of Chrysosplenosides I and A (CAs 1 & 2), flavonol glycosides from the Xizang medicinal plant Chrysosplenium axillare Maxim., which exhibit potent anti-aging effects on ISCs. Our research, using Drosophila models, reveals that CAs 1 & 2 treatments not only restrain excessive ISC proliferation, thereby preserving intestinal homeostasis, but also extend the lifespan of aging Drosophila. In aged mouse intestinal organoids, CAs 1 & 2 enhance the growth and budding of intestinal organoids, indicating improved regenerative capacity. Mechanistic investigations show that CAs 1 & 2 exert their effects by activating the peroxisome proliferator-activated receptor-gamma (PPARγ) and concurrently inhibiting the epidermal growth factor receptor (EGFR) signaling pathways. Our findings position CAs 1 & 2 as promising candidates for ameliorating ISC aging and suggest that targeting PPARγ, in particular, may offer a therapeutic strategy to counteract age-related intestinal dysfunction.
The Value of the Tip-Apex Distance in Predicting Failure of Fixation of Peritrochanteric Fractures of the Hip
Authors: George David A. , Whittingham-Jones Paul 
Published: June 2024

Abstract
The tip-apex distance philosophy was first coined by Baumgaertner and his coauthors in 1995, in response to failures of fixation following the use of a sliding hip screw in trochanteric fractures of the hip. The use of the tip-apex distance, based upon intraoperative anteroposterior and lateral radiographs, describes the sum of the distance from the tip of the lag screw to the apex of the femoral head, with a recommended combined value of less than 25 mm. Anything above this was shown to be highly predictive of failure by cutout of the lag screw (P = 0.0001).
How Long Does a Hip Replacement Last?
Authors: Twaij Haider , Memon Kashif , Raza Naeem 
Published: June 2024

Abstract
Total hip arthroplasty (THA) is a successful procedure used to manage hip osteoarthritis (OA). This study aims to identify the lifespan of a prosthesis to better inform patients. It looks at two data sets consisting of (1) a systematic review and meta-analysis conducted using Embase and Medline searches to September 2017 that provided data for forty-four series (13 212 THAs) and (2) a pooled survival and implant data from the National Joint Registry (NJR) for ninety-two series (215 676 THAs) from Australia and Finland. The twenty-five-year pooled survival of THAs from the case series was 77.6% (95% CI: 76.0–79.2) compared to 57.9% (95% CI: 57.1–58.7) from the NJR. Data from the NJR are less likely to be biased; thus patients and surgeons can expect a THA to last twenty-five years in around 58% of patients.
UK DRAFFT
Authors: Mitchell Chris , Karia Monil , Domos Peter 
Published: June 2024

Abstract
The Distal Radius Acute Fracture Fixation Trial (DRAFFT) is a multicenter randomized trial that compared the functional outcomes of Kirschner wire (K-wire) fixation vs. open reduction and internal fixation (ORIF) with locking plate for dorsally displaced extra-articular distal radius fractures that were reducible using a closed technique. Functional outcomes, radiographic parameters, and cost effectiveness were compared at twelve months postoperatively. Locking plates were associated with statistically significant improvement in radiographic findings of unknown clinical significance. No statistically significant difference in functional outcomes was seen at twelve-month follow-up. There was significant cost savings for K-wiring vs. ORIF.