bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequency of nut consumption and mortality risk in the PREDIMED nutrition intervention trial
Authors: Guasch-Ferré Marta , Bulló Mònica , Martínez-González Miguel Ángel , Ros Emilio , Corella Dolores , Estruch Ramon , Fitó Montserrat , Arós Fernando , Wärnberg Julia , Fiol Miquel , Lapetra José , Vinyoles Ernest , Lamuela-Raventós Rosa Maria , Serra-Majem Lluís , Pintó Xavier , Ruiz-Gutiérrez Valentina , Basora Josep , Salas-Salvadó Jordi 
Published: July 2013

AbstractBackgroundProspective studies in non-Mediterranean populations have consistently related increasing nut consumption to lower coronary heart disease mortality. A small protective effect on all-cause and cancer mortality has also been suggested. To examine the association between frequency of nut consumption and mortality in individuals at high cardiovascular risk from Spain, a Mediterranean country with a relatively high average nut intake per person.MethodsWe evaluated 7,216 men and women aged 55 to 80 years randomized to 1 of 3 interventions (Mediterranean diets supplemented with nuts or olive oil and control diet) in the PREDIMED (‘PREvención con DIeta MEDiterránea’) study. Nut consumption was assessed at baseline and mortality was ascertained by medical records and linkage to the National Death Index. Multivariable-adjusted Cox regression and multivariable analyses with generalized estimating equation models were used to assess the association between yearly repeated measurements of nut consumption and mortality.ResultsDuring a median follow-up of 4.8 years, 323 total deaths, 81 cardiovascular deaths and 130 cancer deaths occurred. Nut consumption was associated with a significantly reduced risk of all-cause mortality (Pfor trend <0.05, all). Compared to non-consumers, subjects consuming nuts >3 servings/week (32% of the cohort) had a 39% lower mortality risk (hazard ratio (HR) 0.61; 95% CI 0.45 to 0.83). A similar protective effect against cardiovascular and cancer mortality was observed. Participants allocated to the Mediterranean diet with nuts group who consumed nuts >3 servings/week at baseline had the lowest total mortality risk (HR 0.37; 95% CI 0.22 to 0.66).ConclusionsIncreased frequency of nut consumption was associated with a significantly reduced risk of mortality in a Mediterranean population at high cardiovascular risk.Please see related commentary:http://www.biomedcentral.com/1741-7015/11/165.Trial registrationClinicaltrials.gov. International Standard Randomized Controlled Trial Number (ISRCTN):35739639. Registration date: 5 October 2005.
Psychiatric symptoms as a clinical presentation of Cushing’s syndrome
Authors: Tang Alice , O’Sullivan Anthony J , Diamond Terry , Gerard Andrew , Campbell Peter 
Published: July 2013

Abstract
Cushing’s syndrome can present with a spectrum of symptoms; however, it is less recognised that psychiatric symptoms can form part of the clinical presenting features. In the investigations for an organic cause for a psychiatric illness, Cushing’s syndrome needs to be considered, especially if there are other features such as hirsutism or hypertension. In this article, the two cases reported demonstrate that a prompt diagnosis is not only important for psychiatric management but also crucial for timely institution of the necessary treatment of life-threatening causes of hypercortisolaemia such as metastatic adrenal carcinoma.
Ultra-pure platelet isolation from canine whole blood
Authors: Trichler Shauna A , Bulla Sandra C , Thomason John , Lunsford Kari V , Bulla Camilo 
Published: July 2013

Abstract

Background
Several research applications involving platelets, such as proteomic and transcriptomic analysis, require samples with very low numbers of contaminating leukocytes, which have considerably higher RNA and protein content than platelets. We sought to develop a platelet purification protocol that would minimize contamination, involve minimal centrifugation steps, and yield highly pure platelet samples derived from low volume whole blood samples from healthy dogs.

Results
Using an optimized OptiPrep density gradient technique, platelet recovery was 51.56% with 99.99% platelet purity and leukocyte contamination of 100 leukocytes per 108 platelets, on average. Platelet samples were subjected to additional purification with CD45-labeled Dynabeads after density barrier centrifugation resulting in a 95-fold depletion of residual leukocytes. Platelets purified using these methods remained inactivated as assessed by Annexin V and P-selectin labeling with flow cytometry.

Conclusions
The use of OptiPrep density gradient is a quick method for obtaining highly purified platelet samples from low volumes of canine whole blood with minimal contamination. Additional depletion of residual leukocytes can be achieved using CD45-labeled beads. These platelet samples can then be used for many downstream applications that require ultra-pure platelet samples such as RNA and protein analysis.
Neuroregeneration and dementia: new treatment options
Authors: Mitran Smaranda Ioana , Catalin Bogdan , Sfredel Veronica , Balseanu Tudor-Adrian 
Published: July 2013

Abstract
In the last years, physiological aging became a general concept that includes all the changes that occur in organism with old age. It is obvious now, that in developing and developed countries, new health problems concerning older population appear. One of these major concerns is probably dementia. Sooner or later, all forms of dementia lead to learning deficit, memory loss, low attention span, impairment of speech and poor problem solving skills. Normal ageing is a physiological process that also involves a lot of neurological disorders with the same type of symptoms and effects that many researchers are trying to minimize in demented patients. In this review we try to highlight some of the newest aspects of therapeutic strategies that can improve natural neuroregeneration.
Non-conventional induction strategies for production of subunit swine erysipelas vaccine antigen in rE. coli fed-batch cultures
Authors: da Silva Adilson José , Horta Antônio Carlos Luperni , Velez Ana Maria , Iemma Mônica Rosas C , Sargo Cíntia Regina , Giordano Raquel LC , Novo Maria Teresa M , Giordano Roberto C , Zangirolami Teresa Cristina 
Published: July 2013

Abstract
In spite of the large number of reports on fed-batch cultivation of E. coli, alternative cultivation/induction strategies remain to be more deeply exploited. Among these strategies, it could be mentioned the use of complex media with combination of different carbon sources, novel induction procedures and feed flow rate control matching the actual cell growth rate. Here, four different carbon source combinations (glucose, glycerol, glucose + glycerol and auto-induction) in batch media formulation were compared. A balanced combination of glucose and glycerol in a complex medium formulation led to: fast growth in the batch-phase; reduced plasmid instability by preventing early expression leakage; and protein volumetric productivity of 0.40 g.L-1.h-1. Alternative induction strategies were also investigated. A mixture of lactose and glycerol as supplementary medium fully induced a high biomass population, reaching a good balance between specific protein production (0.148 gprot.gDCW
-1) and volumetric productivity (0.32 g.L-1.h-1). The auto-induction protocol showed excellent results on specific protein production (0.158 gprot.gDCW
-1) in simple batch cultivations. An automated feed control based on the on-line estimated growth rate was implemented, which allowed cells to grow at higher rates than those generally used to avoid metabolic overflow, without leading to acetate accumulation. Some of the protocols described here may provide a useful alternative to standard cultivation and recombinant protein production processes, depending on the performance index that is expected to be optimized. The protocols using glycerol as carbon source and induction by lactose feeding, or glycerol plus glucose in batch medium and induction by lactose pulse led to rSpaA production in the range of 6 g.L-1, in short fed-batch processes (16 to 20 h) with low accumulation of undesired side metabolites.
Thiol-Containing Polysilsesquioxane Liquid and Photocurable Sulfur-Containing Transparent Organic–Inorganic Hybrid Monoliths Obtained via Cosolvent-Free Hydrolytic Polycondensation
Authors: Igarashi Yuta , Kajihara Koichi , Kanamura Kiyoshi 
Published: July 2013

Abstract
Cosolvent-free hydrolytic polycondensation of trimethoxy(3-sulfanylpropyl)silane under acidic conditions was found to yield thiol-containing polysilsesquioxane (PSQ) liquid. The resultant PSQ liquid is stable at room temperature and serves as a precursor of sulfur-containing transparent organic–inorganic hybrid monoliths prepared via ultraviolet (UV) photoinduced thiol–ene reaction. The factors that determine the UV transparency of the hybrids were investigated. Sulfur-containing organic–inorganic hybrids showing good transparency in the UV spectral region are obtained using a vinyl compound, which is transparent around the UV absorption edge of thiol groups.
Time-Resolved Spectroscopic and Imaging Studies on Laser Ablation of Molecular Systems: From Mechanistic Study to Bio/Nano Applications
Authors: Masuhara Hiroshi 
Published: July 2013

Abstract
Studies on laser ablation of polymer films, molecular crystals in solution, protein solution, and culture media containing living cells are summarized and considered. Dynamics and mechanism of laser ablation were systematically studied by utilizing time-resolved spectroscopy and imaging; femtosecond–nanosecond transient absorption and emission spectroscopy, nanosecond shadowgraphy, nanosecond–nanometer interferometry, femtosecond surface light scattering imaging. It was confirmed by integrating both data that primary processes of laser ablation can be well understood in the framework of Jablonski diagram. For nanosecond laser ablation of doped polymers, it was demonstrated that cyclic multiphoton absorption is an efficient photothermal conversion process leading to photothermal ablation. For femtosecond laser ablation of dye films, transient pressure mechanism was proposed indicating photomechanical ablation. As applications of laser ablation, nanoparticle preparation, protein crystallization, and manipulation of living cells are presented. Laser ablation of molecular crystals in poor solvent gives small fragments whose size are in a few tens nm. The fabricated nanocolloids are stable without adding detergents and their size was the smallest as nanoparticles produced by the top-down-method. Multiphoton laser ablation of water generates local impulsive force due to bubble formation, shockwave propagation, and local convection flow. The force triggers molecular and protein crystallization in their supersaturated solutions, whose mechanisms are described and considered. The impulsive force is also very useful for manipulating living cells and its high potential was confirmed by examining cell functions such as division, differentiation, death, and migration. Finally summary and future plan are presented.
Synthesis of Hollow Zinc Oxide Nanoparticles by Templating Micelles of Poly(styrene-b-acrylic acid-b-ethylene oxide) in Aqueous Solutions
Authors: Zhai Shuo , Yusa Shin-ichi , Nakashima Kenichi 
Published: July 2013

Abstract
Spherical hollow zinc oxide (ZnO) nanoparticles have been successfully synthesized by templating micelles of poly(styrene-b-acrylic acid-b-ethylene oxide) (PS-b-PAA-b-PEO). This polymer forms a micelle with a PS core, a PAA shell, and a PEO corona in aqueous solutions. Zinc chloride (ZnCl2) and sodium hydrogen carbonate (NaHCO3) were used as precursors of ZnO. In the synthesis, the PS core acts as a template for cavities of the hollow particles, the PAA shell is beneficial for arresting zinc ions for further mineralizing, and the PEG corona stabilizes the zinc hydrogen carbonate (Zn(HCO3)2)/polymer nanocomposite to prevent secondary aggregate formation. Hollow ZnO nanoparticles were obtained after the calcination of the separated (Zn(HCO3)2)/polymer nanocomposite at 500 °C for 4 h. The hollow ZnO nanoparticles were characterized by various techniques including transmission electron microscopy and X-ray diffraction analysis. The hollow ZnO particles have a spherical shape, a narrowly distributed size of 24.7 ± 1.3 nm, and a shell thickness of 6.9 ± 0.7 nm. Compared with other methods, the present method gives hollow ZnO nanoparticles with smaller size (ca. 25 nm), narrow size distribution, controlled shape, and robust shell. This result strongly demonstrates the advantages of the present synthesis route.
‘Time + materials: Choreographic experiments with labour and finance in The Dangerologists’ Work Songs’
Authors: Chow Broderick D. V. 
Published: July 2013

Abstract
This article discusses the process of choreographing labour in Work Songs, a practice as research investigation in dance theatre that poses the question: how is labour as such made present and perceptible within the representational frame of the theatre? Work Songs was created between November 2011 and December 2012 by Broderick Chow and Tom Wells (The Dangerologists) and has been seen in London, Manchester, Leeds, Edinburgh and Gießen, Germany. Departing from Karl Marx’s analyses of abstract and concrete labour, the article describes the economic and social context of post-Fordist production and financial capitalism to which this piece is a response. It then discusses the apparatuses of theatre and dance in terms of their ‘erasure’ of labour through, respectively, dramatic representation and naturalized virtuosity. It then argues that the perception of labour as such within the frame of the theatre is directly related to the interlinked dynamics of temporality and materiality – when narrative/chronological temporality is suspended, materiality is made present. This is demonstrated in Work Songs by the insertion of blockages into the smooth flow of its choreography, and the suspension of its dramatic narrative.
Their hands in the dirt: How Kazuo Ohno and Stephanie Skura cultivate dance practices and alterity from nature
Authors: Hamp Amanda E. 
Published: July 2013

Abstract
As city-based artists, Kazuo Ohno and Stephanie Skura each create for themselves excursions into ‘nature’, which are departures from their daily modern, urban lives, plus fecund sources for their artistic practices. Ohno and Skura are part of the tradition in dance of turning to and connecting with nature, but are distinct from it in their practices – and the effects in the practitioner – of embodying and ‘becoming’ images. Paradoxically, Ohno’s and Skura’s embodiment of imagery from nature cultivates experiences of alterity, which yields aesthetics of inclusion.
Short-term estimation and application of biological variation of small dense low-density lipoproteins in healthy individuals
Authors: Lara-Riegos Julio , Brambila Eduardo , Ake-Ku Ana , Villegas-Hernández Vanessa , Quintero-Carrilo Carmen , Ortiz-Andrade Rolffy , Yza-Villanueva Rubén , Torres-Romero Julio , Lozano-Zarain Patricia 
Published: July 2013

Abstract

Background: A number of methods have been developed to measure small dense low-density lipoprotein cholesterol (sd-LDL-C) to evaluate atherogenic risk in the population. However, to our knowledge there are no reports about the biologic variability of these lipoproteins. Therefore, the aim of this work was to estimate sd-LDL-C biological variability, and with this information establish quality specifications, index of individuality (II) and reference change values (RCV).

Methods: To estimate within- and between-subject biological variability, sd-LDL-C in serum was measured in 24 individuals (11 female and 13 male) for 5 consecutive days and then, at 2 and 3 weeks. Quality specifications, II, and RCVs were estimated according to procedures described.

Results: Total within- and between-subject biological variability, expressed as coefficient of variation, was 9.1% and 20%. Meanwhile, within- and between-biological variability in female and men was 10.9% and 6.7%, and 22% and 17%, respectively. Desirable quality specification to the sd-LDL-C method was 4.6% for analytical imprecision, bias 5.5% and total allowable error of 11.4%; the II was 0.46 and the RCV (calculated at 95% and 99% of significance) was 27.1% and 35.7%, for the total data.

Conclusions: Short-term biological variability components were determined, and then used to estimate quality specifications, II and RCV for sd-LDL-C precipitation assay. To our knowledge, this is one of the first reports about sd-LDL-C biological variability, so that this information can be used as a starting point to develop long-term studies of biological variability for sd-LDL-C.
Full-length Vpu and human CD4(372–433) in phospholipid bilayers as seen by magic angle spinning NMR
Authors: Do Hoa Q. , Wittlich Marc , Glück Julian M. , Möckel Luis , Willbold Dieter , Koenig Bernd W. , Heise Henrike 
Published: July 2013

Abstract
HIV-1 Vpu and CD4(372–433), a peptide comprising the transmembrane and cytoplasmic domain of human CD4, were recombinantly expressed in Escherichia coli, uniformly labeled with 13C and 15N isotopes, and separately reconstituted into phospholipid bilayers. Highly resolved dipolar cross-polarization (CP)-based solid-state NMR spectra of the two transmembrane proteins were recorded under magic angle sample spinning. Partial assignment of 13C resonances was achieved. Site-specific assignments were obtained for 13 amino acid residues of CD4(372–433) and two Vpu residues. Additional amino acid type-specific assignments were achieved for 10 amino acid spin systems for both CD4(372–433) and Vpu. Further, structural flexibility was probed with different dipolar recoupling techniques, and the correct insertion of the transmembrane domains into the lipid bilayers was confirmed by proton spin diffusion experiments.
Drug use in Ghana: knowledge, perceptions, and attitudes in a small group of elite student sportspersons
Authors: Brown Ato A. , Asare Pereko Kingsley K. , Eliason Sebastian 
Published: July 2013

Summary
Study aim: This study assessed the level of knowledge, perceptions, and attitudes of tertiary student athletes on doping issues. Emphasis was placed on the knowledge of substances found on the prohibited list of the World Anti- Doping Agency (WADA) as well as the potential effects and side effects of the drugs when consumed.
Material and methods: The study was conducted in elite tertiary student sportspersons who had qualified in their various disciplines to represent Ghana at the 2011 World University Games in Shenzhen, China.
Results: The study found that these sportspersons had significantly higher levels of knowledge of socially abused drugs that also appeared on the WADA prohibited list, such as cocaine (90.9%, p<0.01), heroine (90.9%, p<0.01), cannabis (87.9%, p<0.01) and steroids (72.7%, p<0.05), as compared to drugs that are not socially abused. There was also a large gap in knowledge among respondents with regard to specific knowledge about the possible effects and side effects of banned substance. Of these sportspersons, 30% had received some form of education on doping agents; however, there was no significant statistical difference in knowledge between respondents that had received some form of education and those that had not.
Conclusions: The findings of this study suggest that sporting authorities would have to intensify their efforts to provide tertiary school athletes with credible and up-to-date information on doping issues. These interventions may also need to be extended to the wider sporting populace.
Group reflection and precompact paratopological groups
Authors: Tkachenko Mikhail 
Published: July 2013

AbstractWe construct a precompact completely regular paratopological
Abelian group G of size (2ω)+ such that all subsets
of G of cardinality ≤ 2ω are closed. This shows that Protasov’s
theorem on non-closed discrete subsets of precompact
topological groups cannot be extended to paratopological
groups. We also prove that the group reflection of the
product of an arbitrary family of paratopological (even semitopological)
groups is topologically isomorphic to the product
of the group reflections of the factors, and that the group reflection,
H*, of a dense subgroup G of a paratopological group
G is topologically isomorphic to a subgroup of G*.
Delicious Risk: Progress Lab 1422’s Obstructions as ”Group Dramaturgy”
Authors: Ditor Rachel 
Published: July 2013

ABSTRACT: Dramaturg and director Rachel Ditor examines the Obstructions program at Vancouver’s Progress Lab 1422. The Progress Lab is an organization made up of many of Vancouver’s most popular and well-established physical theatre companies. In the Obstructions program, each company is set the task of creating a performance based upon those aspects of their practice that most challenge them. The performance is then “judged” by their peers. Ditor examines the particular type of risk this represents, as well as the collaborative theatre culture that makes this program possible.
Des registres de rationalité en concurrence? Empirica magiques et médecine scolastique
Authors: Weill-Parot Nicolas 
Published: July 2013

[fr] On peut interpréter l’opposition récurrente des médecins scolastiques aux vetulae et empirici comme un confl it entre niveaux de rationalité. Sont d’abord distingués trois niveaux de rationalité dans les conceptions de la magie, puis sont étudiées successivement les deux voies de justifi cation rationnelle des empirica intégrés à la médecine savante: celle qui avait recours aux propriétés occultes, celle qui convoquait le pouvoir de l’imagination –dans ce second cas, plutôt le pouvoir de l’imagination sur le corps de celui qui imagine qu’un pouvoir sur un autre corps. Les propriétés occultes semblent offrir une solution plus satisfaisante rationnellement mais aussi peut-être théologiquement. L’article s’achève par l’étude d’un cas: la manière dont le médecin valencien Jeroni Torrella dans l’Opus praeclarum de imaginibus astrologicis (1496-c. 1500) est confronté à un empiricum particulièrement diffi cile à rationaliser, ce qui illustre, à une date tardive, les enjeux de la rationalisation naturaliste scolastique.
Innovation et expérimentation en lycée technique, bilan et perspective
Authors: Coulardeau Jacques 
Published: July 2013

Bilan de trois ans de recherche-action dans le cadre de la Mission académique « Réussir en Seconde », dépendant elle-même de la Mission académique « Réussir au Lycée » de l’Académie de Lille. Nous partons de l’innovation technologique ; photocopieuse, informatique, multimédia, vidéo... Ensuite, l’axe anglais de spécialité permet d’accrocher l’enseignement de l’anglais à des problématiques quotidiennes des élèves. Il s’agit ici de concevoir le terme d’anglais de spécialité dans une acception la plus large possible, permettant ainsi d’ouvrir une gamme importante de discours et de sujets qui permettent à l’élève de se projeter et de se retrouver dans les matières et les sujets enseignés. Puis nous abordons l’innovation pédagogique et de l’émergence de l’autonomie et d’une pédagogie centrée sur l’apprenant et les conséquences que cela a dans la pratique quotidienne. Enfin, nous posons l’innovation cognitive qui nous amène à construire une approche multistratégique remédiante dans la pratique pédagogique actuelle.
The use of cartoons in teaching commercial English
Authors: Tee Anderson Pauline 
Published: July 2013

L’importance de critères affectifs dans l’apprentissage d’une langue étrangère a conduit à la mise au point d’activités ludiques destinées à augmenter le plaisir de l’apprenant. Les dessins humoristiques font partie des documents qui peuvent s’utiliser dans la didactique de l’anglais commercial : non seulement pour développer les compétences linguistiques ainsi que les connaissances des affaires des étudiants, mais aussi pour encourager leur participation et leur créativité. L’approche utilisée dépend en partie du type d’humour concerné.