bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penggunaan Video Edukasi Menyikat Gigi terhadap Pengetahuan dan Keterampilan Pada Siswa Sekolah Dasar
Authors: Supriatna Agus , Widyastuti Nugraheni , Wahyudadi Badai Septa , Rahmah Nur Aulia 
Published: July 2024

Penyuluhan kesehatan gigi pada anak sekolah merupakan salah satu strategi efektif dalam mencegah terjadinya penyakit gigi dan mulut. Penyuluhan kesehatan gigi merupakan tindakan pencegahan primer yang dilakukan sebelum terjadinya suatu penyakit. Salah satu upaya yang dapat dilakukan untuk meningkatkan pengetahuan dan keterampilan anak sekolah dalam menyikat gigi yaitu dengan penggunaan media audio visual. Media ini tidak hanya efektif dalam menyampaikan informasi secara langsung, tetapi juga dapat dijadikan alat penyuluhan yang dapat disebarkan secara luas. Penggunaan teknologi ini dapat meningkatkan daya serap dan pemahaman audiens, terutama pada anak-anak yang cenderung lebih responsif terhadap media visual. Tujuan penelitian ini adalah untuk mengetahui pengaruh penggunaan video edukasi menyikat gigi terhadap pengetahuan dan keterampilan siswa SD Berbt. Muhammdiyah Berua Kota Makassar. Jenis penelitian ini adalah penelitian kuantitatif dengan pendekatan quasy experiment. Teknik pengambilan sampel yang digunakan adalah total sampling pada semua kelas 6 SD Berbt. Muhammadiyah II Berua sebanyak 36 siswa. Penggunaan video edukasi menyikat gigi secara signifikan meningkatkan pengetahuan siswa SD tentang pentingnya menjaga Kesehatan gigi dan mulut dan penggunaan video edukasi juga secara signifikan meningkatakan keterampilan praktis siswa dalam menyikat gigi.
Effects of evaporative conditions on the performance of recovered residue of modified emulsified asphalt
Authors: Huang Haibing , Xu Zujie , Zeng Enjiu , Liu Bin , Xu Wen 
Published: July 2024

Emulsified asphalt has become a popular green material in modern road construction due to its environmentally friendly and energy-saving characteristics. The method of obtaining residues is crucial for accurately evaluating the performance of emulsified asphalt and ensuring the quality of road construction. The evaporated residues of 3% and 6% SBR modified emulsified asphalt and 6% SBS modified emulsified asphalt were obtained by the low-temperature evaporation (LTE) method and the direct heating (DH) method, respectively. The rheological properties of the modified asphalt residues were tested by the temperature frequency sweeping (TFS), multi-stress creep recovery (MSCR), and linear amplitude sweeping (LAS) tests using the dynamic shear rheometer (DSR), and their aging resistance in the form of carbonyl index was measured by the Fourier infrared spectroscopy (FTIR). The test results showed that, as compared to the DH method, the SBR modified emulsified asphalt residue obtained by the LTE method had higher elastic recovery rate, lower irrecoverable creep compliance, smaller accumulated performance deformation, longer fatigue life, and higher aging resistance, indicating that the LTE method using the low-temperature curing conditions (25°C, 24 h—60°C, 24 h) is more suitable for curing the SBR modified emulsified asphalt. However, the opposite trends of the performance indices except the aging resistance were observed for the SBS modified emulsified asphalt residue obtained from the LTE method, showing that the high-temperature curing condition (120°C, 30 min—163°C, 1 min) can give the SBS modified asphalt residue better rheological properties and higher fatigue resistance performance, but it also has the disadvantage of weakening the aging resistance. Therefore, in practical application, an appropriate curing temperature should be selected to condition the SBS modified emulsified asphalt for balancing its rheological performance and aging resistance.
Genetic diversity of Plasmodium malariae in sub-Saharan Africa: a two-marker genotyping approach for molecular epidemiological studies
Authors: Rodi Miriam , Kawecka Katarzyna , Stephan Laura , Berner Lilith , Medina Martha Salinas , Lalremruata Albert , Woldearegai Tamirat Gebru , Matsiegui Pierre Blaise , Groger Mirjam , Manego Rella Zoleko , Ekoka Mbassi Dorothea , Mombo-Ngoma Ghyslain , Agnandji Selidji Todagbe , Ramharter Michael , Mordmüller Benjamin , Inoue Juliana , Held Jana 
Published: July 2024

IntroductionPlasmodium malariae is the most common non-falciparum species in sub-Saharan Africa. Despite this, data on its genetic diversity is scarce. Therefore, we aimed to establish a P. malariae genotyping approach based on size polymorphic regions that can be easily applied in molecular epidemiological studies.MethodsFour potential genotyping markers, Pm02, Pm09, P. malariae thrombospondin-related anonymous protein (pmtrap), and P. malariae merozoite surface protein fragment 2 (pmmsp1 F2) were amplified via nested PCR and analysed using automated capillary gel electrophoresis.ResultsWe observed the highest allelic diversity for pmtrap (MOI = 1.61) and pmmsp1 F2 (He = 0.81). Further applying the two markers pmtrap and pmmsp1 F2 on a different sample set of 21 P. malariae positive individuals followed up over one week, we saw a high consistency in their performance. The results show a large complexity and high dynamics of P. malariae infections in the asymptomatic Gabonese study population.DiscussionWe successfully implemented a new genotyping panel for P. malariae consisting of only two markers: pmtrap and pmmsp1 F2. It can be easily applied in other settings to investigate the genotype diversity of P. malariae populations, providing further important data on the molecular epidemiology of this parasite species.
Comparative analysis of gut microbiota in children with obstructive sleep apnea: assessing the efficacy of 16S rRNA gene sequencing in metabolic function prediction based on weight status
Authors: Chuang Hai-Hua , Huang Chung-Guei , Chou Shih-Hsuan , Li Hsueh-Yu , Lee Chin-Chia , Lee Li-Ang 
Published: July 2024

BackgroundAnalyzing bacterial microbiomes consistently using next-generation sequencing (NGS) is challenging due to the diversity of synthetic platforms for 16S rRNA genes and their analytical pipelines. This study compares the efficacy of full-length (V1–V9 hypervariable regions) and partial-length (V3–V4 hypervariable regions) sequencing of synthetic 16S rRNA genes from human gut microbiomes, with a focus on childhood obesity.MethodsIn this observational and comparative study, we explored the differences between these two sequencing methods in taxonomic categorization and weight status prediction among twelve children with obstructive sleep apnea.ResultsThe full-length NGS method by Pacbio® identified 118 genera and 248 species in the V1–V9 regions, all with a 0% unclassified rate. In contrast, the partial-length NGS method by Illumina® detected 142 genera (with a 39% unclassified rate) and 6 species (with a 99% unclassified rate) in the V3–V4 regions. These approaches showed marked differences in gut microbiome composition and functional predictions. The full-length method distinguished between obese and non-obese children using the Firmicutes/Bacteroidetes ratio, a known obesity marker (p = 0.046), whereas the partial-length method was less conclusive (p = 0.075). Additionally, out of 73 metabolic pathways identified through full-length sequencing, 35 (48%) were associated with level 1 metabolism, compared to 28 of 61 pathways (46%) identified through the partial-length method. The full-length NGS also highlighted complex associations between body mass index z-score, three bacterial species (Bacteroides ovatus, Bifidobacterium pseudocatenulatum, and Streptococcus parasanguinis ATCC 15912), and 17 metabolic pathways. Both sequencing techniques revealed relationships between gut microbiota composition and OSA-related parameters, with full-length sequencing offering more comprehensive insights into associated metabolic pathways than the V3–V4 technique.ConclusionThese findings highlight disparities in NGS-based assessments, emphasizing the value of full-length NGS with amplicon sequence variant analysis for clinical gut microbiome research. They underscore the importance of considering methodological differences in future meta-analyses.
Keratoconus and inflammatory bowel disease: mendelian randomization
Authors: Jin Yiheng , Wang Yuanfeng , Qiu Xu , Liu Jiao , Qu Shugen 
Published: July 2024

BackgroundKeratoconus is a diseased corneal dilation of unknown etiology. Studies have shown that inflammation may play a role in keratoconus. Inflammatory enteritis (IBD), including ulcerative colitis (UC), is a chronic, systemic inflammatory disease. We used Mendelian randomization to assess the causal relationship among IBD, UC and keratoconus.MethodsThe instrumental variable of IBD and UC was selected, the information of the instrumental variable in keratoconus outcome was extracted, and the causal relationship was assessed by the inverse variance weighted method by primary analysis, and its relevant sensitivity analysis.ResultsA causal relationship between IBD and keratoconus was observed significantly (P = 0.017, OR = 1.21, 95% CI = 1.03–1.41), and same as to UC and keratoconus (P = 0.038, OR = 1.25, 95% CI = 1.01–1.54).ConclusionIBD may play a causal role in the development of keratoconus, but the mechanism needs to be further elucidated.
Conduction and validation of a novel mitotic spindle assembly related signature in hepatocellular carcinoma: prognostic prediction, tumor immune microenvironment and drug susceptibility
Authors: Zhang Zhao , Zhang Yuezhou , Hu Gangli , Wu Qianxue , Zhou Yang , Luo Fang 
Published: July 2024

Introduction: We have developed a risk-scoring model using gene expression levels related to mitotic spindle assembly (MSA) to predict the prognosis of liver cancer.Methods and results: Initially, we identified 470 genes related to MSA from public databases. Subsequently, through analysis of sequencing data from liver cancer patient samples in online databases, we identified 7 genes suitable for constructing the risk-scoring model. We validated the predictive accuracy and clinical utility of the model. Through drug sensitivity analysis, we identified SAC3D1 as a gene sensitive to the most common anti-tumor drugs among these 7 genes. We propose SAC3D1 as a significant target for future clinical treatment. Furthermore, we conducted in vivo and in vitro experiments to validate the relevance of SAC3D1 to MSA and found its significant impact on the PI3K/Akt signaling pathway and spindle function.Conclusion: Our research introduces a novel risk-scoring model that accurately predicts liver cancer prognosis. Additionally, our findings suggest SAC3D1 as a promising therapeutic target for hepatocellular carcinoma, potentially revealing new mechanisms underlying liver cancer development.
The impact of AIM2 inflammasome-induced pyroptosis on acute gouty arthritis and asymptomatic hyperuricemia patients
Authors: Chu Jiyan , Tian Jing , Li Ping , Fu Diyu , Guo Lin , Sun Rui 
Published: July 2024

ObjectiveThis study aimed to evaluate the role of absent in melanoma 2 (AIM2) inflammasome-mediated pyroptosis in the pathogenesis of acute gouty arthritis (AGA) and asymptomatic hyperuricemia(AHU).MethodsA cohort of 30 AGA patients, 30 AHU individuals, and 30 healthy controls (HC) was assembled. Demographic and biochemical data, along with blood samples, were collected. Serum double-stranded DNA (dsDNA) levels were quantified using a fluorescent assay. Transcriptomic and proteomic analysis of AIM2, Caspase-1, GSDMD, IL-1β, and IL-18 in peripheral blood mononuclear cells was performed using qRT-PCR and Western blot. Enzyme-linked immunosorbent assay (ELISA) was employed to measure serum IL-1β and IL-18. Spearman correlation analysis was utilized to assess relationships between variables.ResultsBoth AGA and AHU groups demonstrated elevated metabolic indicators and serum levels of dsDNA, IL-1β, and IL-18 compared to the HC group. AGA patients exhibited higher inflammatory markers than the AHU group. In the AGA group, there was a significant increase in the mRNA and protein levels of AIM2, Caspase-1, GSDMD, IL-1β, and IL-18 (P&lt;0.05 to P&lt;0.001). The AHU group showed higher AIM2, Caspase-1, GSDMD, and IL-18 mRNA levels than the HC group (P&lt;0.001 to P&lt;0.01), with a non-significant increase in AIM2, GSDMD, and IL-1β proteins (P&gt;0.05). In contrast, Caspase-1 and IL-18 proteins were significantly higher in the AHU group (P&lt;0.05). Notable correlations were observed between AIM2 protein expression and levels of Caspase-1 and GSDMD in both AGA and AHU groups. In the AGA group, AIM2 protein correlated with IL-1β, but not in the AHU group. The AIM2 protein in the AHU group was positively associated with IL-18, with no such correlation in the AGA group.ConclusionAIM2 inflammasome may play a role in the inflammatory processes of AGA and AHU and that its activation may be related to the pyroptosis pathway.
iPSC-derived NK cells expressing high-affinity IgG Fc receptor fusion CD64/16A to mediate flexible, multi-tumor antigen targeting for lymphoma
Authors: Dixon Kate J. , Snyder Kristin M. , Khaw Melissa , Hullsiek Robert , Davis Zachary B. , Matson Anders W. , Shirinbak Soheila , Hancock Bryan , Bjordahl Ryan , Hosking Martin , Miller Jeffrey S. , Valamehr Bahram , Wu Jianming , Walcheck Bruce 
Published: July 2024

IntroductionNK cells can mediate tumor cell killing by natural cytotoxicity and by antibody-dependent cell-mediated cytotoxicity (ADCC), an anti-tumor mechanism mediated through the IgG Fc receptor CD16A (FcγRIIIA). CD16A polymorphisms conferring increased affinity for IgG positively correlate with clinical outcomes during monoclonal antibody therapy for lymphoma, linking increased binding affinity with increased therapeutic potential via ADCC. We have previously reported on the FcγR fusion CD64/16A consisting of the extracellular region of CD64 (FcγRI), a high-affinity Fc receptor normally expressed by myeloid cells, and the transmembrane/cytoplasmic regions of CD16A, to create a highly potent and novel activating fusion receptor. Here, we evaluate the therapeutic potential of engineered induced pluripotent stem cell (iPSC)-derived NK (iNK) cells expressing CD64/16A as an “off-the-shelf”, antibody-armed cellular therapy product with multi-antigen targeting potential.MethodsiNK cells were generated from iPSCs engineered to express CD64/16A and an interleukin (IL)-15/IL-15Rα fusion (IL-15RF) protein for cytokine independence. iNK cells and peripheral blood NK cells were expanded using irradiated K562-mbIL21–41BBL feeder cells to examine in in vitro and in vivo assays using the Raji lymphoma cell line. ADCC was evaluated in real-time by IncuCyte assays and using a xenograft mouse model with high circulating levels of human IgG.ResultsOur data show that CD64/16A expressing iNK cells can mediate potent anti-tumor activity against human B cell lymphoma. In particular, (i) under suboptimal conditions, including low antibody concentrations and low effector-to-target ratios, iNK-CD64/16A cells mediate ADCC, (ii) iNK-CD64/16A cells can be pre-loaded with tumor-targeting antibodies (arming) to elicit ADCC, (iii) armed iNK-CD64/16A cells can be repurposed with additional antibodies to target new tumor antigens, and (iv) cryopreserved, armed iNK-CD64/16A are capable of sustained ADCC in a tumor xenograft model under saturating levels of human IgG.DiscussioniNK-CD64/16A cells allow for a flexible use of antibodies (antibody arming and antibody targeting), and an “off-the-shelf” platform for multi-antigen recognition to overcome limitations of adoptive cell therapies expressing fixed antigen receptors leading to cancer relapse due to antigen escape variants.
Impacts of phytoplankton availability on bigeye tuna (Thunnus obesus) recruitment in the Indian Ocean
Authors: Wang Yang , Zhang Yuying , Geng Zhe , Zhu Jiangfeng 
Published: July 2024

IntroductionA sustainable fishery relies on consistent and substantial recruitment. There is, however, considerably high mortality among fish larvae during their early development. One of the most important factors determining larval survival is foraging success. Bigeye tuna is among the most important commercial species in the Indian Ocean. Despite being routinely researched and assessed, it remains unclear how food availability affects the recruitment success of bigeye tuna.MethodsIn this study, we used phytoplankton size (MD50) as an indicator of prey availability and investigated the connection between MD50 and bigeye tuna recruitment from 2000 to 2018 through the Beverton-Holt stock-recruitment function. The Indian Ocean was divided into four regions to accommodate spatial differences.ResultsAs a result, larger MD50 could bring higher recruitment, particularly in the eastern and southern Indian Ocean. Based on the analysis, we inferred that for bigeye tuna, the eastern Indian Ocean is the primary spawning ground, and the peak spawning period falls in Season 2 (April-June).DiscussionThe conclusions provide valuable insights for understanding the recruitment characteristics of bigeye tuna in the Indian Ocean.
Vascular development analysis: a study for tertiary anti-vascular endothelial growth factor therapy after second reactivation of retinopathy of prematurity
Authors: Zhang Xuerui , Peng Jie , Yang Yuan , Liu Yongqing , Zhang Wenting , Gu Victoria Y. , Liu Huanyu , Xiao Haodong , Yin Jiawei , Xu Yu , Zhao Peiquan 
Published: July 2024

PurposeTo observe the vascular development results of tertiary anti-vascular endothelial growth factor (anti-VEGF) therapy following spontaneous second reactivation of retinopathy of prematurity (ROP).MethodsThis retrospective study included 22 infants (42 eyes) with Type 1 or aggressive ROP (A-ROP) who received three anti-VEGF drug treatments for ROP from January 2018 to December 2022. The vascular growth, possible associated risk factors, and the retinal vascularization (DB/DF ratio) were assessed.ResultsThe mean follow-up was 17.6 months. After the 3rd intravitreal injection, seven eyes showed complete vascularization (Group 1), while the remaining 35 eyes demonstrated persistent avascular retina (PAR) (Group 2). In Group 2, 17 eyes maintained a stable state and were classified in the regression subgroup. The other 18 eyes developed a 3rd reactivation (reactivation subgroup) and were treated with laser photocoagulation (LPC).Birth weight (BW) was significantly lower in Group 2 than in Group 1 (p &lt; 0.001). The decision tree analysis shows that only infants weighing more than 1,250 g (17.50%) had a chance to achieve complete retinal vascularization. The possibility of PAR was higher in patients with BW &lt;1,250 g than ≥1,250 g (70.00% vs. 12.50%). In addition, most infants with BW ≥ 1,290 g and initial ROP disease in Zone I or posterior Zone II developed PAR.ConclusionTertiary IVR can successfully treat a second ROP reactivation and improve peripheral retinal vascularization. BW is the most significant factor related to complete retinal vascularization. Our decision tree model may be helpful in predicting the prognosis of anti-VEGF drugs in the event of a second ROP reactivation.
The sub-MIC selective window decreases along the digestive tract: determination of the minimal selective concentration of oxytetracycline in sterilised intestinal contents
Authors: Imazaki Pedro Henrique , Voisin Bertille , Arpaillange Nathalie , Roques Béatrice B. , Dordet-Frisoni Emilie , Dupouy Véronique , Ferran Aude A. , Bousquet-Mélou Alain , Bibbal Delphine 
Published: July 2024

IntroductionThe administration of antibiotics can expose the digestive microbiota of humans and animals to sub-inhibitory concentrations, potentially favouring the selection of resistant bacteria. The minimal selective concentration (MSC) is a key indicator to understand this process. The MSC is defined as the lowest concentration of an antibiotic that promotes the growth of a resistant strain over a susceptible isogenic strain. It represents the lower limit of the sub-minimal inhibitory concentration (MIC) selective window, where resistant mutants can be selected. Previous studies focused on determining the MSC under standard culture conditions, whereas our research aimed to determine the MSC in a model that approximates in vivo conditions.MethodsWe investigated the MSC of oxytetracycline (OTC) in Mueller-Hinton broth (MHB) and sterilised intestinal contents (SIC) from the jejunum, caecum and rectum (faeces) of pigs, using two isogenic strains of Escherichia coli (one susceptible and one resistant to OTC). Additionally, the MIC of OTC against the susceptible strain was determined to assess the upper limit of the sub-MIC selective window.ResultsOur study took a novel approach, and the results indicated that MIC and MSC values were lower in MHB than in SIC. In the latter, these values varied depending on the intestinal segment, with distal compartments exhibiting higher MIC and MSC values. Moreover, the sub-MIC selective window of OTC in SIC narrowed from the jejunum to the rectum, with a significantly closer MSC to MIC in faecal SIC.DiscussionThe results suggest that OTC binds to digestive contents, reducing the fraction of free OTC. However, binding alone does not fully explain our results, and interactions between bacteria and intestinal contents may play a role. Furthermore, our findings provide initial estimates of low concentrations facilitating resistance selection in the gut. Finally, this research enhances the understanding of antimicrobial resistance selection, emphasising the intricate interplay between antibiotics and intestinal content composition in assessing the risk of resistance development in the gut.
Protective effects of Lactococcus lactis subsp. lactis HFY14 supplementation on the brain, intestines, and motor function of antibiotic-treated mice
Authors: Yang Yang , Zhao Yuanji , Lei Huan 
Published: July 2024

IntroductionThis study aimed to explore the anti-oxidative and anti-inflammatory properties of Lactococcus lactis subsp. lactis HFY14 (LLSLHFY14) and investigate its effects on the intestinal barrier, cranial nerve, and motor function in mice treated with antibiotics.MethodsMice were administered an antibiotic mixture (neomycin 5 mg/mL, vancomycin 25 mg/mL, amphotericin B 0.1 mg/mL, ampicillin 10 mg/mL, metronidazole file 5 mg/mL, and lipopolysaccharide 1.5 μg/mL) intraperitoneally, and oxidative stress and inflammatory markers in the serum and brain tissues, and liver index were measured. H&amp;E staining was performed to detect pathological alterations in brain tissues. The expression of intestinal-barrier-related genes and that of genes involved in inflammatory pathways in the brain were detected using polymerase chain reaction (PCR).ResultsLLSLHFY14 administration extended the weight-loaded swimming and running times of mice and decreased the liver index. Moreover, the levels of malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) in the serum and brain tissue were reduced, whereas those of superoxide dismutase (SOD), glutathione (GSH), and interleukin-10 (IL-10) were elevated. Elevated brain expression of the protein kinase B (AKT)/cAMP-response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF)/extracellular signal-regulated kinase 1 (ERK1) pathway, decreased brain expression of the IL-6 gene, and elevated cecum expression of zonula occludens-1 (ZO-1), occludin-1, and claudin-1 genes were noted. LLSLHFY14 supplementation significantly increased Bacteroidetes expression but decreased Firmicutes expression, thus increasing the Bacteroidetes/Firmicutes ratio.DiscussionOverall, LLSLHFY14 supplementation ameliorated antibiotic-induced oxidative stress and inflammation in the mouse central nervous system, intestinal barrier dysfunction, and increased motor function, thus confirming its potential application as probiotics.