bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insomnia and accidents: cross‐sectional study (EQUINOX) on sleep‐related home, work and car accidents in 5293 subjects with insomnia from 10 countries
Authors: Léger Damien , Bayon Virginie , Ohayon Maurice M. , Philip Pierre , Ement Philippe , Metlaine Arnaud , Chennaoui Mounir , Faraut Brice 
Published: November 2013

SummaryThe link between sleepiness and the risk of motor vehicle accidents is well known, but little is understood regarding the risk of home, work and car accidents of subjects with insomnia. An international cross‐sectional survey was conducted across 10 countries in a population of subjects with sleep disturbances. Primary care physicians administered a questionnaire that included assessment of sociodemographic characteristics, sleep disturbance and accidents (motor vehicle, work and home) related to sleep problems to each subject. Insomnia was defined using the International Classification of Sleep Disorders (ICSD‐10) criteria. A total of 5293 subjects were included in the study, of whom 20.9% reported having had at least one home accident within the past 12 months, 10.1% at least one work accident, 9% reported having fallen asleep while driving at least once and 4.1% reported having had at least one car accident related to their sleepiness. All types of accident were reported more commonly by subjects living in urban compared to other residential areas. Car accidents were reported more commonly by employed subjects, whereas home injuries were reported more frequently by the unemployed. Car accidents were reported more frequently by males than by females, whereas home accidents were reported more commonly by females. Patients with insomnia have high rates of home accidents, car accidents and work accidents related to sleep disturbances independently of any adverse effects of hypnotic treatments. Reduced total sleep time may be one factor explaining the high risk of accidents in individuals who complain of insomnia.
Evaluation of Aldosterone Concentrations in Dogs with Hypoadrenocorticism
Authors: Baumstark M.E. , Sieber‐Ruckstuhl N.S. , Müller C. , Wenger M. , Boretti F.S. , Reusch C.E. 
Published: November 2013

BackgroundSome dogs with primary hypoadrenocorticism (HA) have normal sodium and potassium concentrations, a phenomenon called atypical Addison's disease. The assumption that the zona glomerulosa and aldosterone secretion in these dogs are normal seems widely accepted; however, aldosterone measurements are missing in most published cases.ObjectivesTo measure aldosterone in dogs withHAwith and without electrolyte abnormalities and to determine the time point of aldosterone peak concentrations duringACTHstimulation.AnimalsSeventy dogs withHA, 22 dogs with diseases mimickingHA, and 19 healthy dogs.MethodsProspective study. Blood samples were taken before and 60 minutes after injection of 250 μgACTHin all dogs. Additional blood samples were taken 15, 30, and 45 minutes afterACTHin 7 dogs withHAand in 22 with diseases mimickingHA.ResultsBaseline andACTH‐stimulated aldosterone was significantly lower in dogs withHAthan in the other groups. Aldosterone was low or undetectable in 67/70 dogs withHAindependently of sodium and potassium levels. In 3 dogs, sodium/potassium concentrations were normal; in 1 dog, sodium was normal and potassium decreased. In all 4,ACTH‐stimulated aldosterone concentrations were below the detection limit of the assay. Aldosterone concentrations were not different at 30, 45, or 60 minutes afterACTHadministration.Conclusion and Clinical ImportanceCortisol and aldosterone secretion is compromised in dogs withHAwith and without electrolyte abnormalities. The term atypical Addison's disease, used for dogs with primaryHAand normal electrolytes, must be reconsidered; other mechanisms allowing normal electrolyte balance without aldosterone should be evaluated in these dogs.
Arterial Thromboembolism in 250 Cats in General Practice: 2004–2012
Authors: Borgeat K. , Wright J. , Garrod O. , Payne J.R. , Fuentes V.L. 
Published: November 2013

BackgroundPopulation characteristics and outcome of cats with arterial thromboembolism (ATE) managed in general practice (GP) have been poorly described.HypothesisCats withATEpresenting toGPare usually euthanized at presentation, but survival times >1 year are possible.AnimalsCats withATEmanaged by 3GPclinics in the United Kingdom.MethodsRecords of cases presenting toGPover a 98‐month period (2004–2012) were reviewed. Cats with an antemortem diagnosis of limbATEwere included. Outcome information was obtained.ResultsOver 98 months, 250 cats were identified withATE. Prevalence was approximately 0.3%. At presentation, 153 cats (61.2%) were euthanized, with 68/97 (70.1%) of the remaining cats (27.2% of the total population) surviving >24 hours after presentation. Of these, 30/68 (44.1%) survived for at least 7 days. Hypothermia (HR, 1.44; 95%CI, 1.002–2.07;P = .049) and management by Clinic 2 (HR, 5.53; 95%CI, 1.23–24.8;P = .026) were independent predictors of 24‐hour euthanasia or death. For cats surviving >24 hours, hypothermia (HR, 2.25; 95% CI, 1.12–4.48;P = .021) and failure to receive aspirin, clopidogrel, or both (HR, 8.26; 95%CI, 1.39–50;P = .001) were independent predictors of euthanasia or death within 7 days. For cats that survived ≥7 days, median survival time was 94 (95%CI, 42–164) days, with 6 cats alive 1 year after presentation.ConclusionsAlthough 153/250 cats were euthanized at presentation, 6 cats survived >12 months. No factors were identified that predicted euthanasia on presentation.
Intranasal dexmedetomidine vs midazolam for premedication in children undergoing complete dental rehabilitation: a double‐blinded randomized controlled trial
Authors: Sheta Saad A. , Al‐Sarheed Maha A. , Abdelhalim Ashraf A. 
Published: November 2013

SummaryBackgroundThis prospective, randomized, double‐blind study was designed to evaluate the use of intranasally administered dexmedetomidine vs intranasal midazolam as a premedication in children undergoing complete dental rehabilitation.MethodsSeventy‐two children of American Society of Anesthesiology classification (ASA) physical status (I & II), aged 3–6 years, were randomly assigned to one of two equal groups. Group M received intranasal midazolam (0.2 mg·kg−1), and group D received intranasal dexmedetomidine (1 μg·kg−1). The patients' sedation status, mask acceptance, and hemodynamic parameters were recorded by an observer until anesthesia induction. Recovery conditions, postoperative pain, and postoperative agitation were also recorded.ResultsThe median onset of sedation was significantly shorter in group M 15 (10–25) min than in group D 25 (20–40) min (P = 0.001). Compared with the children in group M, those in group D were significantly more sedated when they were separated from their parents (77.8% vs 44.4%, respectively) (P = 0.002). Satisfactory compliance with mask application was 58.3% in group M vs 80.6% in group D (P = 0.035). The incidences of postoperative agitation and shivering were significantly lower in Group D compared with group M. Thirteen children (36.1%) in group M, showed signs of nasal irritation with teary eyes, and none of these signs was seen in the children in group D (P = 0.000). There were no incidences of bradycardia, hypotension, in either of the groups during study observation.ConclusionIntranasal dexmedetomidine (1 μg·kg−1) is an effective and safe alternative for premedication in children; it resulted in superior sedation in comparison to 0.2 mg·kg−1 intranasal midazolam. However, it has relatively prolonged onset of action.
Artefactual responses of mesophyll conductance to CO2 and irradiance estimated with the variable J and online isotope discrimination methods
Authors: GU LIANHONG , SUN YING 
Published: November 2013

AbstractStudies with the variable J method have reported that mesophyll conductance (gm) rapidly decreases with increasing intercellular CO2 partial pressures (Ci) or decreasing irradiance. Similar responses have been suggested with the online isotope discrimination method, although with less consistency. Here we show that even when the true gm is constant, the variable J method can produce an artefactual dependence of gm on Ci or irradiance similar to those reported in previous studies for any of the following factors: day respiration and chloroplastic CO2 photocompensation point are estimated with Laisk method; Ci or electron transport rate is positively biased; net photosynthetic rate is negatively biased; insufficient NADPH is assumed while insufficient ATP limits RuBP regeneration. The isotopic method produces similar artefacts if fractionation of carboxylation or Ci is positively biased or Δ13 negatively biased. A non‐zero chloroplastic resistance to CO2 movement results in a qualitatively different dependence of gm on Ci or irradiance and this dependence is only sensitive at low Ci. We thus cannot rule out the possibility that previously reported dependence of gm on Ci or irradiance is a methodological artefact. Recommendations are made to take advantage of sensitivities of the variable J and isotopic methods for estimating gm.
Differential cadmium and zinc distribution in relation to their physiological impact in the leaves of the accumulatingZygophyllum fabago L.
Authors: LEFÈVRE ISABELLE , VOGEL‐MIKUŠ KATARINA , JEROMEL LUKA , VAVPETIČ PRIMOŽ , PLANCHON SÉBASTIEN , ARČON IZTOK , ELTEREN JOHANNES T VAN , LEPOINT GILLES , GOBERT SYLVIE , RENAUT JENNY , PELICON PRIMOŽ , LUTTS STANLEY 
Published: November 2013

AbstractCadmium and zinc share many similar physiochemical properties, but their compartmentation, complexation and impact on other mineral element distribution in plant tissues may drastically differ. In this study, we address the impact of 10 μmCd or 50 μmZn treatments on ion distribution in leaves of a metallicolous population of the non‐hyperaccumulating speciesZygophyllum fabagoat tissue and cell level, and the consequences on the plant response through a combined physiological, proteomic and metabolite approach. Micro‐proton‐induced X‐ray emission and laser ablation inductively coupled mass spectrometry analyses indicated hot spots ofCd concentrations in the vicinity of vascular bundles in response toCd treatment, essentially bound toS‐containing compounds as revealed by extended X‐ray absorption fine structure and non‐protein thiol compounds analyses. A preferential accumulation ofZn occurred in vascular bundle and spongy mesophyll in response toZn treatment, and was mainly bound toO/N‐ligands. Leaf proteomics and physiological status evidenced a protection of photosynthetically active tissues and the maintenance of cell turgor through specific distribution and complexation of toxic ions, reallocation of some essential elements, synthesis of proteins involved in photosynthetic apparatus orC‐metabolism, and metabolite synthesis with some specificities regarding the considered heavy metal treatment.
Complete Genome Sequences of Five Paenibacillus larvae Bacteriophages
Authors: Sheflo Michael A. , Gardner Adam V. , Merrill Bryan D. , Fisher Joshua N. B. , Lunt Bryce L. , Breakwell Donald P. , Grose Julianne H. , Burnett Sandra H. 
Published: November 2013

ABSTRACT

Paenibacillus larvae
is a pathogen of honeybees that causes American foulbrood (AFB). We isolated bacteriophages from soil containing bee debris collected near beehives in Utah. We announce five high-quality complete genome sequences, which represent the first completed genome sequences submitted to GenBank for any
P. larvae
bacteriophage.
Complete Genome Sequence of Staphylococcus aureus Siphovirus Phage JS01
Authors: Jia Hongying , Bai Qinqin , Yang Yongchun , Yao Huochun 
Published: November 2013

ABSTRACT

Staphylococcus aureus
is the most prevalent and economically significant pathogen causing bovine mastitis. We isolated and characterized one staphylophage from the milk of mastitis-affected cattle and sequenced its genome. Transmission electron microscopy (TEM) observation shows that it belongs to the family
Siphovirus
. We announce here its complete genome sequence and report major findings from the genomic analysis.
Draft Genome Sequence of Geobacillus thermoleovorans Strain B23
Authors: Boonmak Chanita , Takahasi Yasunori , Morikawa Masaaki 
Published: November 2013

ABSTRACT

Here, we report the draft genome sequence of
Geobacillus thermoleovorans
strain B23, which was isolated from a deep subterranean petroleum reservoir in Japan. An array of genes related to unique long-chain alkane degradation pathways in
G. thermoleovorans
B23 has been identified by whole-genome analyses of this strain.
Impact of the urgent care telephone service NHS 111 pilot sites: a controlled before and after study
Authors: Turner J , O'Cathain A , Knowles E , Nicholl J 
Published: November 2013

ObjectivesTo measure the impact of the urgent care telephone service NHS 111 on the emergency and urgent care system.DesignControlled before and after study using routine data.SettingFour pilot sites and three control sites covering a total population of 3.6 million in England, UK.Participants and dataRoutine data on 36 months of use of emergency ambulance service calls and incidents, emergency department attendances, urgent care contacts (general practice (GP) out of hours, walk in and urgent care centres) and calls to the telephone triage service NHS direct.InterventionNHS 111, a new 24 h 7 day a week telephone service for non-emergency health problems, operated by trained non-clinical call handlers with clinical support from nurse advisors, using NHS Pathways software to triage calls to different services and home care.Main outcomesChanges in use of emergency and urgent care services.ResultsNHS 111 triaged 277 163 calls in the first year of operation for a population of 1.8 million. There was no change overall in emergency ambulance calls, emergency department attendances or urgent care use. There was a 19.3% reduction in calls to NHS Direct (95% CI −24.6% to −14.0%) and a 2.9% increase in emergency ambulance incidents (95% CI 1.0% to 4.8%). There was an increase in activity overall in the emergency and urgent care system in each site ranging 4.7–12%/month and this remained when assuming that NHS 111 will eventually take all NHS Direct and GP out of hours calls.ConclusionsIn its first year of operation in four pilot sites NHS 111 did not deliver the expected system benefits of reducing calls to the 999 ambulance service or shifting patients to urgent rather than emergency care. There is potential that this type of service increases overall demand for urgent care.
What happens when patients know more than their doctors? Experiences of health interactions after diabetes patient education: a qualitative patient-led study
Authors: Snow Rosamund , Humphrey Charlotte , Sandall Jane 
Published: November 2013

ObjectiveTo explore the impact of patient education on the lives of people with diabetes, including the effect on interactions with doctors and other healthcare professionals.DesignQualitative user-led study using longitudinal interviews and 146 h of participant observation. Data were analysed using a narrative approach.Participants21 patients with type 1 diabetes, those either about to attend a patient education course or those who had completed the course in the previous 10 years.SettingEstablished patient education centres in three UK teaching hospitals teaching the Dose Adjustment for Normal Eating (DAFNE) course.ResultsBoth postcourse and several years later, most participants spoke of the experience of taking part in education as life-changingly positive. It helped them understand how to gain control over a very complex disease and freed them from dependence on medical advice and restrictive regimes. However, interactions within the health system following patient education could be fraught. Participants emerged from the course with greater condition-specific knowledge than many of the healthcare professionals they encountered. When these professionals did not understand what their patients were trying to do and were uncomfortable trusting their expertise, there could be serious consequences for these patients' ability to continue effective self-management.ConclusionsPatients who have in-depth knowledge of their condition encounter problems when their expertise is seen as inappropriate in standard healthcare interactions, and expertise taught to patients in one branch of medicine can be considered non-compliant by those who are not specialists in that field. Although patient education can give people confidence in their own self-management skills, it cannot solve the power imbalance that remains when a generalist healthcare professional, however well meaning, blocks access to medication and supplies needed to manage chronic diseases successfully. There is a role for those involved in primary and hospital care, including those supporting and training healthcare professionals, to recognise these problems and find ways to acknowledge and respect chronic patients' biomedical and practical expertise.
Exploring the efficacy of cyclic vs static aspiration in a cerebral thrombectomy model: an initial proof of concept study
Authors: Simon Scott , Grey Casey Paul , Massenzo Trisha , Simpson David G , Longest P Worth 
Published: November 2013

Background and purposeCurrent technology for endovascular thrombectomy in ischemic stroke utilizes static loading and is successful in approximately 85% of cases. Existing technology uses either static suction (applied via a continuous pump or syringe) or flow arrest with a proximal balloon. In this paper we evaluate the potential of cyclic loading in aspiration thrombectomy.MethodsIn order to evaluate the efficacy of cyclic aspiration, a model was created using a Penumbra aspiration system, three-way valve and Penumbra 5Max catheter. Synthetic clots were aspirated at different frequencies and using different aspiration mediums. Success or failure of clot removal and time were recorded. All statistical analyses were based on either a one-way or two-way analysis of variance, Holm–Sidak pairwise multiple comparison procedure (α=0.05).ResultsCyclic aspiration outperformed static aspiration in overall clot removal and removal speed (p<0.001). Within cyclic aspiration, Max Hz frequencies (∼6.3 Hz) cleared clots faster than 1 Hz (p<0.001) and 2 Hz (p=0.024). Loading cycle dynamics (specific pressure waveforms) affected speed and overall clearance (p<0.001). Water as the aspiration medium was more effective at clearing clots than air (p=0.019).ConclusionsCyclic aspiration significantly outperformed static aspiration in speed and overall clearance of synthetic clots in our experimental model. Within cyclic aspiration, efficacy is improved by increasing cycle frequency, utilizing specific pressure cycle waveforms and using water rather than air as the aspiration medium. These findings provide a starting point for altering existing thrombectomy technology or perhaps the development of new technologies with higher recanalization rates.
Republished: Environmental and occupational exposure to chemicals and telomere length in human studies
Authors: Zhang Xiao , Lin Shao , Funk William E , Hou Lifang 
Published: November 2013

Abstract
Telomeres are complexes of tandem repeats of DNA (5′-TTAGGG-3′) and protein that cap eukaryotic chromosomes and play a critical role in chromosome stability. Telomeres shorten with aging and this process can be accelerated by increased oxidative stress and episodes of inflammation. Evidence is rapidly growing that telomere length (TL) may be affected by environmental chemicals that have frequently been associated with chronic diseases. In this article, we review the published data on TL in relation to environmental and occupational exposure to several chemicals based on our own and others' studies. The environmental and occupational exposures associated with shorter TL include traffic-related air pollution (ie, particulate matter (PM), black carbon (BC), and benzene and toluene), polycyclic aromatic hydrocarbons (PAHs), N-nitrosamines, pesticides, lead, exposure in car mechanical workshops, and hazardous waste exposure. Arsenic, persistent organic pollutants (POPs) and short-term exposure to PM are associated with longer TL. We discuss the possible reasons for the differences in results, including time- and dose-related issues, study design, and possible mechanisms involved in telomere regulation. We also discuss the future directions and challenges for TL-related environmental and occupational health research, such as investigation of TL in subpopulations of blood leukocytes, and the study of genetic and epigenetic factors that may regulate telomere integrity using longitudinal designs.
Hallmarks of Aromatase Inhibitor Drug Resistance Revealed by Epigenetic Profiling in Breast Cancer
Authors: Jansen Maurice P.H.M. , Knijnenburg Theo , Reijm Esther A. , Simon Iris , Kerkhoven Ron , Droog Marjolein , Velds Arno , van Laere Steven , Dirix Luc , Alexi Xanthippi , Foekens John A. , Wessels Lodewyk , Linn Sabine C. , Berns Els M.J.J. , Zwart Wilbert 
Published: November 2013

Abstract
Aromatase inhibitors are the major first-line treatment of estrogen receptor–positive breast cancer, but resistance to treatment is common. To date, no biomarkers have been validated clinically to guide subsequent therapy in these patients. In this study, we mapped the genome-wide chromatin-binding profiles of estrogen receptor α (ERα), along with the epigenetic modifications H3K4me3 and H3K27me3, that are responsible for determining gene transcription (n = 12). Differential binding patterns of ERα, H3K4me3, and H3K27me3 were enriched between patients with good or poor outcomes after aromatase inhibition. ERα and H3K27me3 patterns were validated in an additional independent set of breast cancer cases (n = 10). We coupled these patterns to array-based proximal gene expression and progression-free survival data derived from a further independent cohort of 72 aromatase inhibitor–treated patients. Through this approach, we determined that the ERα and H3K27me3 profiles predicted the treatment outcomes for first-line aromatase inhibitors. In contrast, the H3K4me3 pattern identified was not similarly informative. The classification potential of these genes was only partially preserved in a cohort of 101 patients who received first-line tamoxifen treatment, suggesting some treatment selectivity in patient classification. Cancer Res; 73(22); 6632–41. ©2013 AACR.