bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of bifidobacteria in the hydrolysis of chlorogenic acid
Authors: Raimondi Stefano , Anighoro Andrew , Quartieri Andrea , Amaretti Alberto , Tomás‐Barberán Francisco A. , Rastelli Giulio , Rossi Maddalena 
Published: December 2014

AbstractThis study aimed to explore the capability of potentially probiotic bifidobacteria to hydrolyze chlorogenic acid into caffeic acid (CA), and to recognize the enzymes involved in this reaction. Bifidobacterium strains belonging to eight species occurring in the human gut were screened. The hydrolysis seemed peculiar of Bifidobacterium animalis, whereas the other species failed to release CA. Intracellular feruloyl esterase activity capable of hydrolyzing chlorogenic acid was detected only in B. animalis. In silico research among bifidobacteria esterases identified Balat_0669 as the cytosolic enzyme likely responsible of CA release in B. animalis. Comparative modeling of Balat_0669 and molecular docking studies support its role in chlorogenic acid hydrolysis. Expression, purification, and functional characterization of Balat_0669 in Escherichia coli were obtained as further validation. A possible role of B. animalis in the activation of hydroxycinnamic acids was demonstrated and new perspectives were opened in the development of new probiotics, specifically selected for the enhanced bioconversion of phytochemicals into bioactive compounds.
Deutsche Bergbautechnik für einen gigantischen Streb im Nordpolarkreis
Authors: Burbaum Thomas , Simeonova Yana , Nurgaleev Talgat 
Published: December 2014

AbstractIm Herbst 2011 hat das Bergwerk “Worgaschorskaja” den größten Streb im Polarkreis in Betrieb genommen und fördert inzwischen bereits aus seiner dritten Bauhöhe. Die Bergleute bezeichnen diesen Streb als “Riesen”, der mit seiner technischen Ausrüstung und Größe beeindruckt. Der Bericht gibt einen kurzen Überblick über die Strebausrüstung und die Fördertechnik.
Design of an allosterically regulated retroaldolase
Authors: Raymond Elizabeth A. , Mack Korrie L. , Yoon Jennifer H. , Moroz Olesia V. , Moroz Yurii S. , Korendovych Ivan V. 
Published: December 2014

AbstractWe employed a minimalist approach for design of an allosterically controlled retroaldolase. Introduction of a single lysine residue into the nonenzymatic protein calmodulin led to a 15,000‐fold increase in the second order rate constant for retroaldol reaction with methodol as a substrate. The resulting catalyst AlleyCatR is active enough for subsequent directed evolution in crude cell bacterial lysates. AlleyCatR's activity is allosterically regulated by Ca2+ ions. No catalysis is observed in the absence of the metal ion. The increase in catalytic activity originates from the hydrophobic interaction of the substrate (∼2000‐fold) and the change in the apparent pKa of the active lysine residue.
Kinetics and thermodynamics of metal‐binding to histone deacetylase 8
Authors: Kim Byungchul , Pithadia Amit S. , Fierke Carol A. 
Published: December 2014

AbstractHistone deacetylase 8 (HDAC8) was originally classified as a Zn(II)‐dependent deacetylase on the basis of Zn(II)‐dependent HDAC8 activity in vitro and illumination of a Zn(II) bound to the active site. However, in vitro measurements demonstrated that HDAC8 has higher activity with a bound Fe(II) than Zn(II), although Fe(II)‐HDAC8 rapidly loses activity under aerobic conditions. These data suggest that in the cell HDAC8 could be activated by either Zn(II) or Fe(II). Here we detail the kinetics, thermodynamics, and selectivity of Zn(II) and Fe(II) binding to HDAC8. To this end, we have developed a fluorescence anisotropy assay using fluorescein‐labeled suberoylanilide hydroxamic acid (fl‐SAHA). fl‐SAHA binds specifically to metal‐bound HDAC8 with affinities comparable to SAHA. To measure the metal affinity of HDAC, metal binding was coupled to fl‐SAHA and assayed from the observed change in anisotropy. The metal KD values for HDAC8 are significantly different, ranging from picomolar to micromolar for Zn(II) and Fe(II), respectively. Unexpectedly, the Fe(II) and Zn(II) dissociation rate constants from HDAC8 are comparable, koff ∼0.0006 s−1, suggesting that the apparent association rate constant for Fe(II) is slow (∼3 × 103 M−1 s−1). Furthermore, monovalent cations (K+ or Na+) that bind to HDAC8 decrease the dissociation rate constant of Zn(II) by ≥100‐fold for K+ and ≥10‐fold for Na+, suggesting a possible mechanism for regulating metal exchange in vivo. The HDAC8 metal affinities are comparable to the readily exchangeable Zn(II) and Fe(II) concentrations in cells, consistent with either or both metal cofactors activating HDAC8.
Role of membrane contact sites in protein import into mitochondria
Authors: Horvath Susanne E. , Rampelt Heike , Oeljeklaus Silke , Warscheid Bettina , van der Laan Martin , Pfanner Nikolaus 
Published: December 2014

ABSTRACTMitochondria import more than 1,000 different proteins from the cytosol. The proteins are synthesized as precursors on cytosolic ribosomes and are translocated by protein transport machineries of the mitochondrial membranes. Five main pathways for protein import into mitochondria have been identified. Most pathways use the translocase of the outer mitochondrial membrane (TOM) as the entry gate into mitochondria. Depending on specific signals contained in the precursors, the proteins are subsequently transferred to different intramitochondrial translocases. In this article, we discuss the connection between protein import and mitochondrial membrane architecture. Mitochondria possess two membranes. It is a long‐standing question how contact sites between outer and inner membranes are formed and which role the contact sites play in the translocation of precursor proteins. A major translocation contact site is formed between the TOM complex and the presequence translocase of the inner membrane (TIM23 complex), promoting transfer of presequence‐carrying preproteins to the mitochondrial inner membrane and matrix. Recent findings led to the identification of contact sites that involve the mitochondrial contact site and cristae organizing system (MICOS) of the inner membrane. MICOS plays a dual role. It is crucial for maintaining the inner membrane cristae architecture and forms contacts sites to the outer membrane that promote translocation of precursor proteins into the intermembrane space and outer membrane of mitochondria. The view is emerging that the mitochondrial protein translocases do not function as independent units, but are embedded in a network of interactions with machineries that control mitochondrial activity and architecture.
Glycerol transformation to value added C3 diols: reaction mechanism, kinetic, and engineering aspects
Authors: Vasiliadou Efterpi S. , Lemonidou Angeliki A. 
Published: December 2014

Glycerol can serve as a starting material for the production of a variety of chemicals currently formed via fossil‐based routes. The selective hydro‐deoxygenation to C3‐diols is one of the most attractive methods for glycerol upgrading. 1,2‐ and 1,3‐Propanediols which are the target products of this reaction are high added value chemicals with a wide range of applications. The reaction mechanisms for both routes (1,2‐ and 1,3‐diols) are presented and analyzed including the strategies followed for mechanistic understanding. 1,2‐Propanediol is proposed to be formed via two mechanisms namely dehydration–hydrogenation and dehydrogenation‐dehydration–hydrogenation. The selective conversion of glycerol to 1,3‐propanediol is suggested to proceed through dehydration–hydrogenation and direct hydrogenolysis mechanisms. The reaction mechanism depends on various factors such as the catalyst formulation, acid and basic character of the system and the H2 origin. A section focusing on engineering issues describes and compares the reaction mode ie batch‐continuous and liquid–gas phase operation. The different reaction configurations have an impact mainly on product distribution and catalyst stability, which is greatly improved when continuous operation is applied. In addition, the impact of reaction temperature, hydrogen pressure, glycerol concentration as well as the reaction kinetics are also discussed. The approach of operating under inert conditions with H2 generated in situ seems to be a very promising concept for process intensification. WIREs Energy Environ 2015, 4:486–520. doi: 10.1002/wene.159This article is categorized under:

Bioenergy > Science and Materials
Synthesis, Crystal Structure, Luminescent Property and Anti‐esophageal Tumor Activity of Dinuclear Zinc Complex Based on meso‐1,2,3,4‐Tetra(1H‐benzo[d]imidazol‐2‐yl)butane
Authors: Pan Rong‐Kai , Liu Sheng‐Gui , Wang Shao‐Xiang , Li Guo‐Bi , Su Wen‐Yi , Huang Qian‐Wen , He Ya‐Mei 
Published: December 2014

AbstractThe complex [Zn2(tbb)Cl4]·4DMF [tbb = meso‐1,2,3,4‐tetra(1H–benzo‐[d]imidazol‐2‐yl)butane] was synthesized and characterized by X‐ray single crystal structure analyses [C44H54N12Cl4O4Zn2, Mr = 1087.53, crystal system, monoclinic, space group, P21/n, a = 9.2307(12), b = 11.3748(14), c = 23.874(3) Å, β = 90.480(2)°]. In the complex, one meso‐1,2,3,4‐tetra(1H‐benzo‐[d]imidazol‐2‐yl)butane coordinates two zinc ions. Both the zinc ions are coordinated in a tetrahedral fashion by two nitrogen atoms of tbb and two chloride ions. The complex emits blue luminescence in DMF solution and exhibits inhibition on the growth of Eca109 cancer cell with IC50 of 22.1 ± 6.7 μM, which was lower than that of cisplatin (62.5 ± 12.4 μM) and ligand (tbb) (24.7 ± 7.8 μM). This complex has potential application in treatment of esophageal cancer.
Structural, Luminescence, and Electronic Properties of Potassium Holmium Cyclotetraphosphate KHoP4O12: Experiment and Theory
Authors: Zhu Jing , Qian Wei , Zhu Zhongqi , Chen Hui 
Published: December 2014

Abstract The chemical preparation, structure, luminescence, and electronic properties are given for a new potassium holmium cyclotetraphosphate KHoP4O12, a promising optics material for applications. Single‐crystal X‐ray diffraction analysis shows that the newly synthesized compound crystallizes in the monoclinic system with space group C2/c and Z = 4. [P4O12]4– anionic rings and HoO8 polyhedra display a three‐dimensional (3D) framework by corner‐sharing. The ten‐coordinated potassium atoms are located in the delimited tunnels. KHoP4O12 exhibits the blue light emission under the excitation of 330 nm. To gain further insights into electronic properties of crystal KHoP4O12, theoretical calculation based on density functional theory (DFT) was performed using the total‐energy code CASTEP with the LDA + U approach. The calculated bandgap with U = 3.0–3.5 eV is in good agreement with the experimental measurement.
Implications of the Notch1‐Snail/Slug‐epithelial to mesenchymal transition axis for lymph node metastasis in infiltrating ductal carcinoma
Authors: Cao Yu‐Wen , Wan Guo‐Xing , Sun Jian‐Ping , Cui Xiao‐Bin , Hu Jian‐Ming , Liang Wei‐Hua , Zheng Yu‐Qin , Li Wen‐Qin , Li Feng 
Published: December 2014

AbstractEmerging evidence suggests that activation of the Notch1 signaling pathway inducing epithelial to mesenchymal transition (EMT) mediated by Snail/Slug promotes invasion and metastasis of breast cancer cells in vitro. However, the implication of the Notch1‐Snail/Slug‐EMT axis in breast cancer patients remains unclear. A total of 200 formalin‐fixed paraffin‐embedded samples of invasive ductal carcinoma (IDC), and 37 adjacent non‐neoplastic tissue (ANNT) samples from patients who had not been treated with neoadjuvant therapy were examined. Expression of Notch1, Slug, Snail, E‐cadherin, N‐cadherin, and vimentin was determined by immunohistochemistry on tissue microarrays (TMAs). The correlation between protein expression and clinicopathological characteristics of breast cancer patients was also evaluated. Results showed that a significantly high percentage of cases with high expression of Notch1 (74%, 148/200), Slug (36%, 72/200), Snail (62%, 124/200), and N‐cadherin (77%, 153/200) and a low percentage of cases with high expression of E‐cadherin (27%, 54/200) were observed in IDC compared to those in ANNTs. High Notch1, Slug, Snail, and N‐cadherin expression and low E‐cadherin expression in patients with IDC were significantly correlated with lymph node metastasis. In addition, correlation analysis results revealed that high Notch1 expression was significantly associated with high Slug, Snail, and N‐cadherin expression and low E‐cadherin expression in IDC. Furthermore, a high Snail expression was significantly associated with low E‐cadherin expression, and a high Slug expression was found to be significantly associated with increased N‐cadherin expression in patients with IDC. Hence, our study suggested that the Notch1‐Snail/Slug‐EMT axis may be implicated in the lymph node metastasis affecting patients with IDC.
Directly dated MIS 3 Lake-Level Record from Lake Manix, Mojave Desert, California, USA
Authors: Reheis Marith C. , Miller David M. , McGeehin John P. , Redwine Joanna R. , Oviatt Charles G. , Bright Jordon 
Published: December 2014

AbstractAn outcrop-based lake-level curve, constrained by ~ 70 calibrated14C ages onAnodontashells, indicates at least 8 highstands between 45 and 25 cal ka BP within 10 m of the 543-m upper threshold of Lake Manix in the Mojave Desert of southern California. Correlations of Manix highstands with ice, marine, and speleothem records suggest that at least the youngest three highstands coincide with Dansgaard–Oeschger (D–O) stadials and Heinrich events 3 and 4. The lake-level record is consistent with results from speleothem studies in the Southwest that indicate cool wet conditions during D–O stadials. Notably, highstands between 43 and 25 ka apparently occurred at times of generally low levels of pluvial lakes farther north as interpreted from core-based proxies. Mojave lakes may have been supported by tropical moisture sources during oxygen-isotope stage 3, perhaps controlled by southerly deflection of Pacific storm tracks due to weakening of the sea-surface temperature gradient in response to North Atlantic climate perturbations.
Stability analysis of the elliptic cylinder wake
Authors: Leontini Justin S. , Lo Jacono David , Thompson Mark C. 
Published: December 2014

AbstractThis paper presents the results of numerical stability analysis of the wake of an elliptical cylinder. Aspect ratios where the ellipse is longer in the streamwise direction than in the transverse direction are considered. The focus is on the dependence on the aspect ratio of the ellipse of the various bifurcations to three-dimensional flow from the two-dimensional Kármán vortex street. It is shown that the three modes present in the wake of a circular cylinder (modes A, B and QP) are present in the ellipse wake, and that in general they are all stabilized by increasing the aspect ratio of the ellipse. Two new pertinent modes are found: one long-wavelength mode with similarities to mode A, and a second that is only unstable for aspect ratios greater than approximately 1.75, which has similar spatiotemporal symmetries to mode B but has a distinct spatial structure. Results from fully three-dimensional simulations are also presented confirming the existence and growth of these two new modes in the saturated wakes.
Predicting real-time adaptive performance in a dynamic decision-making context
Authors: Good Darren 
Published: December 2014

AbstractIndividuals in organizations must frequently enact a series of ongoing decisions in real-time dynamic contexts. Despite the increasing need for individuals to manage dynamic decision-making demands, we still understand little about individual differences impacting performance in these environments. This paper proposes a new construct applicable to adaptation in such real-time dynamic environments. Cognitive agility is a formative construct measuring the individual capacity to exhibit cognitive flexibility, cognitive openness and focused attention. This study predicts that cognitive agility will impact adaptive performance in a real-time dynamic decision-making microworld computer game called the Networked Fire Chief; a simulation developed to study and train Australian fire fighters. Cognitive agility, operationalized through three distinct methods (performance measures, self-reports and external-rater reports), explained unique variance beyond measures of general intelligence on the total score of adaptive performance in the microworld.
Contract, Work, and Resistance: Boatmen in Early Colonial Eastern India, 1760s–1850s
Authors: Sinha Nitin 
Published: December 2014

AbstractIn the period between the 1760s and the 1850s boatmen were the most important transport workers in early colonial eastern India, at least numerically. Unfortunately, they have received little scholarly attention so far. By looking at the regime of work, which surprisingly had strong bases in the notion of contract from as early as the 1770s, this article explores the nature of work, work organization, and resistance by boatmen. It argues that although work was structured according to the wage or hire-based (thika) contract regime, the social, political, and ecological conditions in which contract operated were equally crucial. The centrality of contract was premised upon how effectively it was enforceable and, in fact, historically enforced. Boatmen being one of the most important “native” groups with which the British were left on their often long journeys, this article suggests that contract helps to understand the formal “structure of work”, and the minute details of the journey help to understand the “world of work”, of which clandestine trade, weather, wind, rain, torrents, tracking, mooring, internal squabbling, and, not least, preparing food were some of the main components.
Joint effects of gray matter atrophy and altered functional connectivity on cognitive deficits in amnestic mild cognitive impairment patients
Authors: Xie C. , Bai F. , Yuan B. , Yu H. , Shi Y. , Yuan Y. , Wu D. , Zhang Z.-S. , Zhang Z.-J. 
Published: December 2014

BackgroundGray matter (GM) atrophy and disrupted intrinsic functional connectivity (IFC) are often present in patients with amnestic mild cognitive impairment (aMCI), which shows high risk of developing into Alzheimer's disease. Little is known, however, about the relationship between GM atrophy and altered IFC, and whether they are related to cognitive decline.MethodA total of 30 aMCI and 26 cognitively normal (CN) subjects were recruited for this study. Optimized voxel-based morphometric and resting-state functional connectivity magnetic resonance imaging approaches were performed to measure the GM volumes (GMVs) and atrophy-related IFC, respectively. Multivariate linear regression analysis was used to examine the effects of GM atrophy and IFC on cognitive performance across subjects, after controlling for the effects of age, education, gender and group.ResultsCompared with CN subjects, aMCI subjects showed significantly reduced GMVs and decreased IFC in the frontal-parietal and medial temporal lobe systems. Multivariate regression analysis further demonstrated that the GMVs and decreased IFC simultaneously affected the cognitive function. Specifically, GMVs were positively correlated with cognitive performances, including global cognition and episodic memory, and showed a strong trend in correlation between GMVs and non-episodic memory, whilst IFC was positively correlated with the above three cognitive measures, across all subjects. In addition, significant correlation was found between GMVs and altered IFC strength across all subjects.ConclusionsOur findings demonstrated that GMVs and IFC jointly contribute to cognitive performance, and combining quantitative information about GMVs and the strength of functional connectivity may serve as an indicator of cognitive deficits in non-demented elderly.
Positive association between Bromelia balansae (Bromeliaceae) and tree seedlings on rocky outcrops of Atlantic forest
Authors: Rocha Fernando Souza , Duarte Leandro da Silva , Waechter Jorge Luiz 
Published: December 2014

Abstract:Plant facilitation can improve the diversity of plant communities in several ecosystems, especially in stressful environments. The establishment of tree species on rocky outcrops can be limited by several biotic and abiotic factors. The richness and abundance of forest trees species on rocky outcrops surrounded by a large forest remnant in south Brazil were correlated with the cover of Bromelia balansae, a spiny stoloniferous species commonly occurring at forest–grassland transitions. Sixty plots were established on three flat rocky outcrops (20 in each). In each plot the coverage of bromeliads was estimated into three classes (0%, 1–50% and 51–100%) and all seedlings (individuals 10–30 cm in height) were counted and identified. ANOVAs with randomization tests were performed to establish the effect of different bromeliad cover classes on the richness and abundance of seedlings. The cover of bromeliads was positively related to the richness and abundance of young pioneer tree species. Evidence suggests that B. balansae can act as a nurse plant for tree seedlings, but manipulative experiments are needed to confirm this.
Gorenstein-duality for one-dimensional almost complete intersections – with an application to non-isolated real singularities
Authors: VAN STRATEN DUCO , WARMT THORSTEN 
Published: December 2014

AbstractWe give a generalisation of the duality of a zero-dimensional complete intersection for the case of one-dimensional almost complete intersections, which results in a Gorenstein module M = I/J. In the real case the resulting pairing has a signature, which we show to be constant under flat deformations. In the special case of a non-isolated real hypersurface singularity f, with a one-dimensional critical locus, we relate the signature on the Jacobian module I/Jf to the Euler characteristic of the positive and negative Milnor fibre, generalising the result for isolated critical points. An application to real curves in ℙ2(ℝ) of even degree is given.
VI—Formulæ and Scheme of Calculation for the Development of a Function of Two Variables in Spherical Harmonics
Authors: Bauschinger T. , Knott C. G. 
Published: December 2014

When a function has been expressed as a series of spherical harmonics with constant coefficients, the determination of these coefficients from given values of the function is in the general case one of the most complicated operations which can be set before the calculator.Since Gauss first carried out these operations in a calculation of this kind, efforts have not been wanting to simplify them and make their frequent application possible. The most successful of all in this respect was Franz Neumann, who showed that by a suitable choice of the argument the calculation could be materially shortened.