bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum Lipid Peroxide Levels in Various Dermatoses
Authors: Demitsu Toshio , Katayama Hiroshi , Yaoita Hideo 
Published: April 2015

AbstractIn studies of physiological roles of lipid peroxide in cutaneous tissue, we examined serum lipid peroxide levels in 199 patients with various dermatoses such as psoriasis, eczema/prurigo, alopecia, bullous disorders, acne/seborrheic dermatitis, atopic dermatitis, SLE, urticaria, progressive systemic sclerosis, generalized morphea, and herpes zoster, by using the TBA (thiobarbituric acid) method and a new assay technique, called the MCDP (methyl carbamoyl‐dimethylamino phenothiazine)‐Hb method. The following results were obtained. By the TBA method, statistically significantly high serum lipid peroxide levels were noted in patients with psoriasis, eczema/prurigo, alopecia, SLE and generalized morphea. By the MCDP‐Hb method, similarly high levels were found in patients with alopecia and atopic dermatitis, compared with those of the control group. The discrepancy between the results from the TBA and the MCDP‐Hb methods is thought to be due to the fact that TBA method measures a secondary product of lipid peroxide, malondialdehyde, whereas the MCDP‐Hb method measures lipid hydroperoxide itself.These results suggest some involvement of lipid peroxidation in the pathogenesis or, at least, in the enhancement and modification of the symptoms in these dermatoses.
A Case of Steatocystoma Simplex on the Head
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Hoshi Kenji , Onda Shutaro 
Published: April 2015

AbstractA 69‐year‐old Caucasian American man had a dome‐shaped tumor on his head without any other involvement. On his first visit, we considered epidermal cyst or trichilemmal cyst clinically; however histopathological features matched those of steatocystoma simplex.
Defective Expression of a Desmosome‐related Keratinocyte Membrane Antigen in Squamous Cell Carcinoma
Authors: Haftek Marek , Meissner Kurt , Viac Jacqueline , Thivolet Jean 
Published: April 2015

AbstractIntercellular junctions of keratinocytes are essential for coordination of the epithelial tissue function. Neoplastic transformation is often accompanied by changes in the glycolipid and glycoprotein composition of the cell membranes, although no evident structural modifications can be observed. We have investigated one aspect of the keratinocyte cell‐cell contact in squamous cell carcinoma using KM48 monoclonal antibody as a differentiation‐related marker for desmosomes. Quantitative analysis of the immunogold labelling revealed that the tumor cells lose the KM48 antigen during their neoplastic transformation. The process could also be observed, although to a lesser degree, in clinically and ultrastructurally normal marginal epidermis. The desmosome frequency in the tumor tissue also decreased, but not as radically as did the KM48 expression. Decreased expression of the desmosome‐related membrane antigen suggests qualitative and perhaps functional modifications of the junction in squamous cell carcinoma.
In vitro and In vivo Regulation of Transglutaminase Activity of Mouse Epidermal Cells by Various Vitamin A Compounds
Authors: Lee Seung Churl , Ikai Kouichi , Ando Yoshihiro , Danno Kiichiro , Imamura Sadao 
Published: April 2015

AbstractIn vitro and in vivo effects of various vitamin A analogues on the transglutaminase activity of mouse epidermal cells were investigated. In vitro addition of all‐trans‐retionic acid (retinoic acid), retinyl acetate, or Ro 10‐9359 (etretinate) to a culture medium induced transglutaminase activity in a transformed mouse epidermal cell line (PAM 212 cells). Induction by a new synthetic vitamin‐A‐like compound (E‐5166, polyprenoic acid derivative) was significant but less marked than that by other vitamin A analogues. Simultaneous addition of retinoic acid and the phorbol ester 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), which also induces transglutaminase, did not cause any increase in transglutaminase activity. These retinoic acid derivatives inhibited the proliferation of PAM 212 cells at concentrations of 10–5–10–6 M in the culture medium.In vivo analysis consisted of oral administration of retinoic acid and Ro 10‐9359 to adult mice. These mice were found to have increased liver transglutaminase activity in the soluble fraction but not in the particulate fraction. Orally administered retinoic acid did not induce transglutaminase activity in the epidermis in the whole skin. These results suggest that retinoids modify transglutaminase activity and epidermal proliferation and differentiation in vitro, but the induction of the enzyme seems not to be related to the clinical effects of orally administered retinoids on various skin diseases.
Analysis of Epidermal Growth Factor Receptor Gene in a Case of Multiple Bowen's Disease
Authors: Tanaka Mizuyo , Dosaka Naoko , Tanaka Toshihiro , Miyachi Yoshiki , Imamura Sadao 
Published: April 2015

AbstractA 45‐year‐old woman had a triple cancer, multiple Bowen's disease, breast cancer, and carcinoma in situ of the cervical portion of her uterus. Development of all three carcinomas was discovered within a period of three years. Southern blotting analysis of the epidermal growth factor receptor (EGFRc) gene of DNA extracted from the individual lesions of multiple Bowen's disease revealed no amplification or variant other than one extra band observed in one lesion. DNA extracted from other patients with solitary Bowen's disease showed essentially the same result. In contrast to squamous cell carcinoma DNA which sometimes has amplification of this gene, our present case suggests that there is only a small possibility that the EGFRc gene plays a role in the carcinogenesis of Bowen's disease.
Scanning Electron Microscopic Appearance of Thy‐1 Positive Dendritic Epidermal Cells in Mouse Epidermal Cell Suspension
Authors: Nakagawa Shojiro , Honma Takayoshi , Kumon Hiromi , Jinno Yoko , Bang Dongsik , Ueki Hiroaki , Ohmori Hiroyuki 
Published: April 2015

AbstractThe distribution of Thy‐1.2 antigens and asialo GM1 on suspended epidermal cells prepared from C3H/He mice by trypsinizing their ear skin was examined by the scanning immunoelectron microscopic method using antibodies against the antigens and antibody‐bacteriophage T4 conjugates as visual markers. Thy‐1.2 and asialo GM1 were found to be distributed diffusely over a cell type with a relatively cuboidal shape and numerous villous projections. These cells are considered to be Thy‐1 positive dendritic epidermal cells. The significance of these findings is discussed.
Cyclic AMP as a Negative Regulator of DNA Synthesis in FRSK Cells, a Fetal Rat Epidermal Cell Line
Authors: Yamanishi Kiyohumi , Kishimoto Saburo , Yasuno Hirokazu 
Published: April 2015

AbstractIn FRSK cells, a cell line derived from fetal rat epidermal cells, cyclic AMP‐elevating agents forskolin (10 μM) and cholera toxin (10 ng/ml) increased cellular cyclic AMP content and suppressed [3H] thymidine incorporation. These effects of forskolin were enhanced by the phosphodiesterase inhibitor 3‐isobutyl‐***l‐methylxanthine (0.1 mM). Dibutyryl cyclic AMP (1 mM), an analog of cyclic AMP, decreased not only basal but also both tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate***‐ and epidermal growth factor‐stimulated [3H] thymidine incorporation. From these results, we suggest that cyclic AMP may be a negative regulatory factor of DNA synthesis in FRSK cells.
Clinical, Histological, and Immunohistological Studies of Postoperative Erythroderma
Authors: Hidano Akira , Yamashita Noriko , Mizuguchi Michi , Toyoda Hiroyuki 
Published: April 2015

AbstractWe report 7 cases of acute fatal illness characterized by fever, diffuse erythematous rash, and progressive leukopenia occurring 10 days after surgical operation. The outcome was uniformly fatal. The biopsy findings consisted of eosinophilic individual necrosis of epidermal cells, satellite cell necrosis, basal liquefaction degeneration, and scanty cell infiltration into the dermis. T lymphocytes were found in the epidermis but Langerhans cells disappeared. These findings are compatible with acute graft‐vs‐host disease following blood transfusion. Explanations based upon drug allergy, infection, toxic shock syndrome, or toxic epidermal necrolysis seem less reasonable.
Cytochemistry of Trichohyalin Granules: A Possible Role for Cornification of Inner Root Sheath Cell in the Hair Follicle
Authors: Sugiyama Sadao 
Published: April 2015

AbstractWe studied ultracytochemically how trichohyalin granules (TGs) were involved in the cornification of the inner root sheath (IRS) cells in the hair follicle. After storing unfixed mouse skin samples in glycerol and then low ionic strength salt solution (G‐LISS), the TGs reduced electron‐dense amorphous material (EDM) to various degrees, with the appearance of filamentous material in routine staining. As the IRS cells were differentiating, these changes in the TGs became successively prominent; Some TGs consisted of the filamentous material and residual granular EDM, showing a “filamentogranular structure”. In the cells in transition to cornified cells, or transitional cells, the EDM of the TGs disappeared and consisted of only filamentous material which was intertwined with keratin filaments (KFs). The internal substructure of the TGs induced by the G‐LISS treatment stained well with ethanolic phosphotungstic acid, which reacts with basic proteins, and the KFs associated with the TGs also stained well. In skin samples not treated with G‐LISS, the TGs of the transitional cells often exhibited fibrogranular structure after PTA staining to detect tissue basic proteins, suggesting a release of PTA stainable proteins(s) by the TGs in living transitional cells. The KFs of the cornified cells were more intensively stained and thicker (about 20 nm wide) than those of the differentiating IRS cells. These findings suggest that the TGs may contain at least two kinds of basic proteins; One is G‐LISS‐soluble, and the other is a very insoluble filamentous protein. These protein materials may be added to or incorporated into the KFs in the cornified cells with the disappearance of the TGs, since the KFs are markedly thick with PTA staining.
Morphea Profunda
Authors: Nakamura Takehiko , Ono Tomomichi , Yamashita Naoko , Maruo Keishi , Mizutari Kiyoko , Ando Masayuki 
Published: April 2015

AbstractA 51‐year‐old Japanese male developed morphea profunda over a plaque of pigmented sweating skin on the left chest extending deeply into the lung. Eight years previously, he noted a brown pigmented spot on the left axilla which started to enlarge over the left chest in a band‐like manner. Later, the involved skin hardened with profuse sweating. Chest X‐ray examination and computer tomography revealed a thickening of the left chest wall and a nodular mass lesion in the left lower lung field. Biopsy specimens from lung, chest wall, and overlying skin revealed a granulomatous tissue in the parenchyma and a marked fibrosis extending from the deep dermis to the pleura. There were no findings suggestive of malignancy or chronic infectious disease. We assume that the process of morphea in this patient extended deeply from the skin to the lung.
A Case of Epidermotropic Metastatic Mammary Carcinoma
Authors: Kato Naoko , Kumakiri Masanobu , Ohkawara Akira 
Published: April 2015

AbstractA special type of metastatic carcinoma to the skin, epidermotropic carcinoma, is thought to be a parasitic occupation of malignant tumors in the epidermis. A patient, a 56‐year‐old Japanese woman who had had a mastectomy because of mammary carcinoma thirteen months earlier, developed a metastatic skin tumor in the operation wound and another area of mammary skin. Histologically, almost only the epidermis was occupied by multifocal metastatic tumor nests, so the tumor was a type of epidermotropic metastatic carcinoma. Moreover, some tumor nests were found to be eliminated from the epidermis. Like other types of metastatic skin tumor, nevus pigmentosus, and amyloidosis, epidermotropic metastatic carcinoma can show transepithelial elimination.
Investigation of the Roles of the Substances in Serum Lipids and Their Constitutive Fatty Acids in Chronic Urticaria
Authors: Kobayashi Sumiko 
Published: April 2015

AbstractThe newly‐generated lipid mediators include products of arachidonate metabolism, prostaglandins and leukotrienes. In this study, serum lipids and fatty acids, including arachidonic acid (C20:4) were examined in 12 normal subjects (6 males and 6 females) and 23 subjects with chronic urticaria (6 males and 17 females), including 17 who made an excellent or good recovery (4 males and 13 females). The results indicated a relationship between chronic urticaria and serum lipids and fatty acids. The ω6 (n‐6) and ω3 (n‐3) series of polyunsaturated fatty acids and lipid peroxidation were suggested that may be one of the mediators in chronic urticaria. Pantethine, glutathione and ascorbic acid were effective in controlling chronic urticaria.
Eczema of the Face, Scalp and Neck: An Epidemiological Comparison by Site
Authors: Goh Chee Leok 
Published: April 2015

AbstractFifteen percent of 3684 patients with eczema attending a contact clinic presented with head and/or neck eczema. There were epidemiological differences among eczema rashes occurring on different parts of the head and neck. Of the 538 patients with head and/or neck eczema, 345 (64%) occurred solely on the face, 52 (10%) on the eyelids, 49 (9%) on the lips, 20 (4%) on the ears, 22 (4%) on the scalp, and 21 (4%) on the neck. Twenty‐nine (5%) had eczema on more than one area of the head/neck. The mean age was lowest in patients with lip eczema (27.5 years) and highest in patients with eyelid eczema (37 years) (p=0.0056). The proportion of females with eczema was higher than males in all groups except the scalp eczema group. Contact dermatitis was more prevalent in the ear eczema (80%) and neck eczema (76%) groups, while endogenous/unclassifiable eczema was more prevalent in the lip eczema (72%) and scalp eczema (73%) groups (p<0.0001). Allergic contact dermatitis was more common than irritant contact dermatitis.
Hyperresponsibility to Exogeneous Interleukin 4 in Atopic Dermatitis
Authors: Furue Masutaka , Ogata Fuyuki , Ootsuki Mamitaro , Ishibashi Yasumasa 
Published: April 2015

AbstractMany humoral and cellular immunological abnormalities have been reported in atopic dermatitis (AD). Since interleukin 4 (IL‐4) enhances IgE production and IgE‐Fc receptor expression by B cells as well as [3H]‐TdR incorporation by T cells, we hypothesized that IL‐4 may play an important role in the regulation of the immune response in AD. We examined [3H]‐TdR incorporation by peripheral blood lymphocytes from AD patients or from non‐AD controls in the presence or absence of IL‐4 or interleukin 2 (IL‐2). We found that IL‐4/IL‐2 responsiveness was significantly higher in AD than controls, although the IgE levels did not seem to be correlated with IL‐4/IL‐2 responsiveness. It is possible that most humoral and cellular immunological abnormalities of AD may be due to this hyperresponsibility to IL‐4
Comparison of Human Hair and Nail Low‐sulfur Protein Compositions on Two‐dimensional Electrophoresis
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractCompositions of human normal hair and nail low‐sulfur proteins were compared using two‐dimensional electrophoresis of their S‐carboxymethylated (SCM) derivatives. Six SCM low‐sulfur protein components with molecular weights (MWs) of 76,000, 73,000, 72,000, 64,000, 61,000 and 55,000 were common to the hair and nail. One component with a MW of 61,000 was specific to hair, and two components, both with a MW of 50,000, were specific to nail.
Correlation of Aedes albopictus Bite Reaction with IgE Antibody Assay and Lymphocyte Transformation Test to Mosquito Salivary Antigens
Authors: Oka Keiko 
Published: April 2015

AbstractTo investigate the mechanisms responsible for mosquito bite reaction, 120 subjects were experimentally exposed to Aedes albopictus bite and two types of immunological tests were performed. Specific IgE antibodies to salivary gland extract (SGE) antigens of female Aedes albopictus were measured by enzyme‐linked immunosorbent assay (ELISA) in all subjects. Lymphocyte transformation tests (LTTs) to the same antigens were measured in 51 of the 120 subjects. The relationships between the results of ELISA and LTTs with bite reactions were statistically analysed. The amount of specific IgE antibodies correlated well with immediate bite reactions (r=0.55) (p<0.01). Stimulation indices (SIs) to SGE antigens correlated with delayed bite reactions (r=0.54) (p<0.01). The results suggest that type I hypersensitivity plays a role in the immediate reaction to mosquito bites and a cellular immune mechanism is responsible for the pathogenesis of delayed bite reactions.
Chemotactic Responsiveness of Eosinophils Isolated from Patients with Inflammatory Skin Diseases
Authors: Morita Eishin , Schröder Jens‐Michael , Christophers Enno 
Published: April 2015

AbstractWe determined the chemotactic responsiveness of peripheral eosinophilic granulocytes (eosinophils) isolated from patients with inflammatory dermatoses and healthy volunteers. Ten patients with atopic dermatitis, five patients with drug reactions, ten patients with psoriasis, and fourteen healthy volunteers were studied. Well characterized chemotaxins, the complement split product C5a, leukotriene B4 (LTB4), platelet activating factor (PAF), and N‐formyl‐methionylleucyl‐phenylalanine (FMLP), were used as chemoattractants. Eosinophils from healthy volunteers showed strong migratory responses towards C5a and PAF but responded poorly to LTB4 and FMLP. When patients were grouped by disease severity, eosinophil chemotactic responses to PAF were significantly enhanced in severely affected patients (p<0.05); this was not true with C5a, LTB4 or FMLP. This enhanced eosinophil chemotaxis to PAF was not related to a specific disease. No correlation between eosinophil chemotactic activity and peripheral blood eosinophil count was observed. The increased responsiveness of circulating eosinophils towards PAF may be related to altered receptor expression during cutaneous inflammation.
Erythema Multiforme in Children: Unusual Clinical Features with Seasonal Occurrence
Authors: Sakurai Michiyo 
Published: April 2015

AbstractEighty‐six cases of erythema multiforme (EM) were studied based on the distribution of skin lesions. Twenty‐nine patients had distinct facial skin lesions as well as lesions on the extremities (Group A) and 57 patients had skin lesions only on the extremities (Group B). Patients in Group A were younger than those in Group B; the average ages being 7.5 and 25.2 years, respectively. In Group A, the disease occurred mainly in winter, while, in Group B, it began most frequently in summer. Preceding ulcerated pernio was seen in 11 patients of Group A, but only one of Group B. Histopathologically, both groups are compatible with a diagnosis of EM, although the seasonal occurrence of facial involvement in Group A may suggest a specific etiological relationship to cold.
Erythroderma with Generalized Lymphadenopathy Induced by Phenytoin
Authors: Danno Kiichiro , Kume Makoto , Ohta Miyuki , Utani Atsushi , Ohno Sayoko , Kobashi Yoichiro 
Published: April 2015

AbstractA 40‐year‐old man developed a generalized erythematous rash, fever, and systemic lymphadenopathy after treatment for two months with anticonvulsants including phenytoin. Laboratory examinations revealed increased white blood cell counts with eosinophilia, abnormal liver function, and increased gammaglobulin levels. A lymphocyte stimulation test showed a significant stimulation index for phenytoin. Skin biopsy findings were nonspecific cellular infiltrates in the upper dermis whereas in lymph nodes the normal architecture was replaced by massive cellular infiltrates consisting largely of lymphocytes, immunoblasts, and eosinophils suggesting a pattern of phenytoin lymphadenopathy. No malignant changes were noted. Phenytoin was discontinued and after a two‐month treatment with oral corticosteroids, all manifestations had nearly subsided. However, the patient should be carefully followed up since phenytoin occasionally induces lymphoproliferative states including malignant lymphoma even after cessation of the medication.
Malignant Hidroacanthoma Simplex
Authors: Takano Yoshika , Nishimura Masayuki , Urabe Atsumichi , Hayashi Noritaka , Toshitani Shoji 
Published: April 2015

AbstractA case of malignant hidroacanthoma simplex is described. Histological examination revealed that the malignant cells had arisen from a tumor cell nest of benign hidroacanthoma simplex and invaded the dermis. Two years and 7 months after the excision of the original thigh lesion, a groin lymph node metastasis was discovered. The metastatic tumor cells in the lymph node were histologically similar to the carcinoma cells in the original thigh lesion and had enzymatic and ultrastructural features resembling eccrine sweat duct.
Phenotypic Heterogeneity of Lymphoma of the Skin
Authors: Nagatani Tetsuo , Kim Shu‐taku , Baba Naoko , Miyamoto Hideaki , Minato Keisuke , Shimoyama Masanori , Nakajima Hiroshi 
Published: April 2015

AbstractWe studied surface markers present in 56 cases of lymphoma of the skin by immunohistochemical staining, using the ABC (avidin‐biotin‐peroxidase complex) and PAP (peroxidase‐antiperoxidase complex) methods. Of these cases, 49 were T‐cell lymphoma and 7 were B‐cell lymphoma. Ten of the 49 cases of T‐cell lymphoma were adult T‐cell leukemia/lymphoma (ATL). Twenty‐five of 31 cases of T‐cell lymphoma except ATL analyzed by the ABC method showed a helper/inducer phenotype (Leu2a−, Leu3a+), two cases showed a suppressor/cytotoxic phenotype (Leu2a+, Leu3a−), one case showed Leu2a+Leu3a+, one case showed an inducer phenotype (Leu2a−, Leu3a+, Leu9+), and one case showed OKT11+, Leu2a−, Leu3a−, Leu1−, Leu9+, CD25+, Leu10+, CD30+. One CD8+ lymphoma was Pagetoid reticulosis, and a CD4+, CD8+ lymphoma was lymphomatoid papulosis with erythematous plaque. Cutaneous T‐cell lymphoma (CTCL), previously described by Edelson et al., is defined as a helper T‐cell lymphoma with marked affinity for the skin. In our study, 5 cases of T‐cell lymphoma of the skin were not CTCL as described by Edelson et al. These results show that T‐cell lymphoma of the skin is heterogeneous in nature. In other words, CTCL is one type but represents a major proportion of T‐cell lymphomas of the skin.
Amino Acid Compositions of Human Hair Fibrous Protein Components Purified with Two‐dimensional Electrophoresis
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractNormal human S‐carboxymethylated (SCM) hair fibrous protein (HFP) components were purified with two‐dimensional electrophoresis, and their amino acid compositions were examined. As previously reported, the SCM cysteine and glycine contents of the crude HFPs were characteristically high and low, respectively, as compared with those reported for the stratum corneum fibrous proteins (SCFPs). However, the SCM cysteine and glycine contents of the purified SCM HFP components were not as high or as low, respectively, but were rather very similar to those of the SCFPs. This suggests that, with respect to cysteine and glycine content, fibrous protein components similar to those of the stratum corneum exist in normal human hair.