bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alzheimer's disease‐causing proline substitutions lead to presenilin 1 aggregation and malfunction
Authors: Ben‐Gedalya Tziona , Moll Lorna , Bejerano‐Sagie Michal , Frere Samuel , Cabral Wayne A , Friedmann‐Morvinski Dinorah , Slutsky Inna , Burstyn‐Cohen Tal , Marini Joan C , Cohen Ehud 
Published: October 2015

AbstractDo different neurodegenerative maladies emanate from the failure of a mutual protein folding mechanism? We have addressed this question by comparing mutational patterns that are linked to the manifestation of distinct neurodegenerative disorders and identified similar neurodegeneration‐linked proline substitutions in the prion protein and in presenilin 1 that underlie the development of a prion disorder and of familial Alzheimer's disease (fAD), respectively. These substitutions were found to prevent the endoplasmic reticulum (ER)‐resident chaperone, cyclophilin B, from assisting presenilin 1 to fold properly, leading to its aggregation, deposition in the ER, reduction of γ‐secretase activity, and impaired mitochondrial distribution and function. Similarly, reduced quantities of the processed, active presenilin 1 were observed in brains of cyclophilin B knockout mice. These discoveries imply that reduced cyclophilin activity contributes to the development of distinct neurodegenerative disorders, propose a novel mechanism for the development of certain fAD cases, and support the emerging theme that this disorder can stem from aberrant presenilin 1 function. This study also points at ER chaperones as targets for the development of counter‐neurodegeneration therapies.
Variability in the detection of macro TSH in different immunoassay systems
Authors: Hattori Naoki , Ishihara Takashi , Shimatsu Akira 
Published: October 2015

DesignMacro TSH is a large molecular-sized TSH that is mostly a complex of TSH and IgG. Patients with macro TSH have elevated serum TSH and normal free thyroxine levels, mimicking subclinical hypothyroidism. The aim of this study was to clarify the degree of cross-reactivity of macro TSH to different commercial immunoassay systems.MethodsScreening for macro TSH was done using a polyethylene glycol (PEG) method and confirmed with gel filtration chromatography in serum samples from 1901 patients with subclinical hypothyroidism. Interference due to human anti-mouse antibodies (HAMA) was examined using HAMA blockers. TSH was measured with an enzyme immunoassay for the analysis of macro TSH. Serum TSH values in patients with macro TSH were also determined with the widely used commercial immunoassay platforms Elecsys, Centaur and Architect, and the detectability of macro TSH was compared among them.ResultsGel filtration chromatography was performed with 174 serum samples with PEG-precipitable TSH ratios >75%. Twenty serum samples were found to contain large molecular-sized TSH, five of which were due to interference by HAMA. The prevalence of macro TSH was eventually 0.79% (15/1901). Commercial immunoassay systems variably recognized macro TSH. The Architect TSH immunoassay platform was the least reactive to macro TSH, but still recognized it in 60% of macro TSH-containing serum samples.ConclusionsThere were no commercial TSH immunoassay platforms that did not cross-react with macro TSH. Screening for macro TSH should be performed before hormone replacement therapy is initiated for subclinical hypothyroidism.
HUMAN INTERFERON MODULATES INFECTED VASCULAR ENDOTHELIUM FUNCTION
Authors: Scheglovitova O. N. , Sklyankina N. N. , Boldyreva N. V. , Babayants A. A. , Frolova I. S. , Kapkaeva M. R. 
Published: October 2015

Background: To study impact of interferon (IFN) α, β and γ on the Herpes simplex virus type 1 (HSV-1) infected endothelial cells functional activity related with participation in the inflammation development. Materials and methods: In the work endothelial cells isolated from umbilical vein were used. Intact and infected cultures were treated by interferon and in the dynamics of cultivation tested mediators in the cultural medium. Results: All investigated interferons activated the production of IL-6. IFN α, β activated the production of IL-8, while IFN γ inhibited her. IFN α and γ increased synthesis of nitrogen oxides and reduced the synthesis of endothelin-1, while IFN β activated the production of endothelin-1. Conclusion: Infection of endothelial cells isolated from umbilical vein with HSV-1 does not alter the ability of interferon in modulating of proinflammatory cyto-kines, nitric oxide and endothelin-1 synthesis. It is obvious in the body modulation manifestations of innate immunity under the influence of exogenous interferon is implemented both intact and infected with HSV-1-vascular endothelium and nature modulation is determined by the type of IFN.
Análisis del impacto sobre la metodología XP de la inclusión de modelos de procesos de negocio en la elicitación de requisitos
Authors: Escobar Villada Andrés Felipe , Velandia Vanegas Diana Lorena , Ordoñez E. Hugo Armando , Cobos Carlos 
Published: October 2015

La metodología ágil de desarrollo de software eXtreme Programming (XP) utiliza Historias de Usuario [HU] como una estrategia de elicitación de requerimientos. Las HU utilizan lenguaje natural, el cual aunque debería ser claro para todos, pues causar confusiones y diferencias en la comprensión de los problemas, entre el equipo de desarrolladores y los interesados [stakeholders]. Este artículo propone incluir el modelamiento de procesos de negocio [PN] en la metodología XP, como una alternativa a las historias de usuario capaz de mejorar la calidad u cantidad de información recolectada. En el desarrollo de esta investigación se evaluó un total de once proyectos (HU vs PN), durante todas las fases propuestas por XP. Las dos estrategias y su efectividad fueron analizadas a través de métricas de software para demostrar el mejoramiento en el desarrollo de los procesos. El trabajo realizado evidenció que el uso de PN mejora la comunicación e incrementa la calidad del proceso y la productividad del equipo de desarrollo.
Le substrat galénique des idées médicales d’Isaac Beeckman (1616-1627)
Authors: Moreau Elisabeth 
Published: October 2015

The Galenic content of Isaac Beeckman's medical ideas (1617-1629) The Journal tenu par Isaac Beeckman de 1604 à 1634 has been studied in the history of the seventeenth-century Scientific Revolution following the theme of Isaac Beeckman's physical mathematical mechanistic view, his proto-molecular theory and his atomistic Lucretian influence. This article goes deeper into the medical ideas of the Journal: how Isaac Beeckman (1588-1637) settles the structure of living matter according to his intensive reading of Galen. It develops a different analysis from the traditionally triumphalist approach in the history of science, focused on the victory of Cartesian mechanism, particularly in the history of medicine taking up Galenism very briefly because of its obsolete physiology. The Galenic corpus inside Isaac Beeckman's Journal consists of the many commentaries of Galen which Beeckman has put down in writing since 1616 until 1627, after when the passages linked to Galen became fewer. Isaac Beeckman's study of Galenic medicine is analyzed according to three approaches: the teleological dimension of Galenism showing up the organic conception of human body corresponding to the divine Providence and consistent with Beeckman's Calvinist belief, the physiologic angle of Galenism, based on natural faculties, stressing thepurely speculative aspect of Beeckman's commentaries, while the pathologic and therapeutic angle supports the Hippocratic humourism influence.
The Analysis of Asymetric Dimethylarginine and Homocysteine in Patients with Chronic Kidney Disease
Authors: Hendrawati Tetty , Bakri Syakib , Arif Mansyur 
Published: October 2015

BACKGROUND: Asymmetric dimethylarginine (ADMA) is a competitive inhibitor of nitric oxide synthase (NOS). ADMA reduces NO synthesis when its concentration elevates. ADMA is a novel risk factor for cardiovascular disease. Plasma ADMA accumulates in patients with endstage renal disease, due to reduced renal clearance. Hyperhomocysteinemia is often found in patients with chronic kidney disease (CKD). Homocysteine may cause ADMA to accumulate; however, the mechanism by which ADMA level elevates in hyperhomocysteinemia is still unclear. Objective of this study was to analyze the concentrations of homocysteine and ADMA and to assess the correlation between homocysteine and ADMA concentrations with the severity of chronic kidney disease.METHODS: This was a cross-sectional study on 75 patients with CKD, comprising men and women aged 40-70 years. Assessments were done on the concentrations of creatinine, homocysteine, ADMA, fasting blood glucose, cholesterol HDL and triglyceride.RESULTS: In later stage of CKD there was significantly higher tHcy concentration as compared with the earlier stage of CKD (p=0.0000). In CKD stage 2 to 4 there was a tendency for ADMA concentration to increase to a significant average (p=0.210), but ADMA concentration was lower at stage 5. There was increased ADMA along with increased tHcy concentration of around 20μ mol/L, and this then decreased. The inverse correlation between tHcy and ADMA concentrations started to appear in CKD stage 4, but this correlation was statistically insignificant (r2 =0.19; p=0.499).CONCLUSIONS: This study showed there was a correlation between homocysteine and ADMA concentrations in patients with CKD stage 2 to 5, although statistically not significant.KEYWORDS: Asymetric Dimethylarginine, Homocysteine, Chronic Kidney Disease
Visfatin and Adiponectin Have an Opposite Correlation with Inflammation and Metabolic Syndrome in Non-Diabetic Obese Indonesian Men
Authors: Meiliana Anna , Lawrence Gatot Susilo , Patellongi Ilhamjaya , Wijaya Andi , As'ad Suryani 
Published: October 2015

BACKGROUND: Along with the increase in obesity is a parallel increase in the prevalence of metabolic complications of obesity, often referred to as the metabolic syndrome (MetS). The role of adipose tissue in MetS has continued to evolve with the description of numerous secretory peptides from adipocytes named adipocytokines or adipokines. Recent studies have found visfatin as the regulation of inflammatory and immunomodulating prosesses, meanwhile adiponectin was known to have a potent anti-inflammatory properties. Here we try to assess the correlation between those two adipokines to MetS, via an inflammatory pathway.METHODS: This was a cross-sectional study on 128 non diabetic obese male subject (waist circumferences ≥90 cm). Visfatin and adiponectin were assessed by ELISA. Statistical analysis was performed using SPSS for Windows v.16.00 with signifcantly p<0.05. The correlations among biomarkers were assessed using Spearman's Rho test.RESULTS: This study showed a significant positive correlation between levels of visfatin and inflammatory markers TNF-α (r=0.22, p<0.005), and hsCRP (r=0.12, p=0.19), significant negative correlation between levels of adiponectin and TNF-α (r=-0.22-8, p<0.005-1), adiponectin and hsCRP (r=-0.14, p=0.11) and visfatin (r=-0.029, p<0.01). Plasma visfatin levels were increased along with the number MetS components, white plasma adiponectin showed inversely relation.CONCLUSION: Our present study has shown that visfatin has a proinflammatory properties and adiponectin has an anti-inflammatory properties, and how they have an opposite effects on MetS. Visfatin was found to have a positive correlaton while adiponectin was found to have a negative correlation with the number of MetS components.KEYWORDS: Obesity, Inflammation, Metabolic Syndrome, Adipocytokines, Visfatin, Adiponectin, TNF-α, hsCRP
Batata 2 Remote Well Pad: Executing Sustainable Development at a Sensitive Amazon Basin Area--Decommission and Abandonment
Authors: Benalcazar Fernando L. , Valdivieso Sebastin 
Published: October 2015

Summary
In Ecuador (circa 2006-07), PetroOriental (operator of Blocks 14 and 17) concluded a large exploration project with the construction of the Batata 2 well pad and the drilling of two exploratory wells, pursuing the confirmation and development of oil reserves identified during the seismic work started in 2003 by EnCanEcuador (subsidiary of EnCana Corporation) in the northern region of Block 14. All this development occurred within the Yasuní National Park, one of the natural areas in South America with the highest biodiversity, and also a traditional land of peoples living in voluntary isolation (the Tagaeri and the Taromenani).
The early planning and execution of this project included the development of a long-term strategy and a set of high-standard social and environmental practices to minimize the company's exposure to social and environmental liabilities and to guarantee the success of this challenging project. Unfortunately, by the first half of 2007, after drilling the previously mentioned exploratory wells, the prospects were declared noncommercial, triggering the process for decommissioning and restoration of the Batata 2 well pad. The loop was closed, and the success of the project was dimmed by the lack of results.
This paper summarizes some of the most important elements of the project planning and execution of the Batata 2 well pad by means of a retrospective analysis with the intent to identify some of those practices, highlight them, and present them to our colleagues in the industry. We now have many examples of life-cycle applications, which is our contribution to the discussion, because we believe that life-cycle assessment and life-cycle management are closely linked to sustainable development, a journey that the oil and gas industry is just starting to make.
Vertical eruption patterns of impacted mandibular third molars after the mesialization of second molars using miniscrews
Authors: Baik Un-Bong , Kook Yoon-Ah , Bayome Mohamed , Park Je-Uk , Park Jae Hyun 
Published: October 2015

ABSTRACT

Objective:
To investigate (1) whether vertical eruption of impacted third molars improves after mesialization of second molars and (2) what factors affect the vertical eruption of impacted third molars when space caused by missing molars is successfully closed by mesialization of the second molar using miniscrews.

Materials and Methods:
The treatment group (Group 1) included 52 patients who had (1) missing mandibular first molars (ML-6) or missing deciduous mandibular second molars (ML-E), (2) initially impacted mandibular third molars, and (3) successful space closure of the edentulous area with orthodontics. Panoramic radiographs at start of treatment (T1) and at time of space closure (T2) were collected. The control group (Group 2) included 46 nonedentulous patients with impacted mandibular third molars without molar protraction treatment. Panoramic radiographs with similar T1/T2 treatment times were selected. Nine measurements were obtained regarding horizontal available space, vertical eruption, and third molar angulation.

Results:
Third molars erupted vertically an average of 2.54 mm in Group 1 compared with 0.41 mm in Group 2. Age, gender, Nolla stage, and angle of the third molars did not show significant correlations with the vertical change of the impacted third molars, whereas the depth of third molar impaction and available space showed significant correlations.

Conclusions:
Impacted mandibular third molars vertically erupt as a result of uprighting with mesialization of the second molar, and vertical eruption is affected by the initial vertical location of impacted third molars and available space.
Protein and small non‐coding RNA‐enriched extracellular vesicles are released by the pathogenic blood fluke Schistosoma mansoni
Authors: Nowacki Fanny C. , Swain Martin T. , Klychnikov Oleg I. , Niazi Umar , Ivens Alasdair , Quintana Juan F. , Hensbergen Paul J. , Hokke Cornelis H. , Buck Amy H. , Hoffmann Karl F. 
Published: October 2015

BackgroundPenetration of skin, migration through tissues and establishment of long‐lived intravascular partners require Schistosoma parasites to successfully manipulate definitive host defences. While previous studies of larval schistosomula have postulated a function for excreted/secreted (E/S) products in initiating these host‐modulatory events, the role of extracellular vesicles (EVs) has yet to be considered. Here, using preparatory ultracentrifugation as well as methodologies to globally analyse both proteins and small non‐coding RNAs (sncRNAs), we conducted the first characterization of Schistosoma mansoni schistosomula EVs and their potential host‐regulatory cargos.ResultsTransmission electron microscopy analysis of EVs isolated from schistosomula in vitro cultures revealed the presence of numerous, 30–100 nm sized exosome‐like vesicles. Proteomic analysis of these vesicles revealed a core set of 109 proteins, including homologs to those previously found enriched in other eukaryotic EVs, as well as hypothetical proteins of high abundance and currently unknown function. Characterization of E/S sncRNAs found within and outside of schistosomula EVs additionally identified the presence of potential gene‐regulatory miRNAs (35 known and 170 potentially novel miRNAs) and tRNA‐derived small RNAs (tsRNAs; nineteen 5′ tsRNAs and fourteen 3′ tsRNAs).ConclusionsThe identification of S. mansoni EVs and the combinatorial protein/sncRNA characterization of their cargo signifies that an important new participant in the complex biology underpinning schistosome/host interactions has now been discovered. Further work defining the role of these schistosomula EVs and the function/stability of intra‐ and extra‐vesicular sncRNA components presents tremendous opportunities for developing novel schistosomiasis diagnostics or interventions.
Les ordinations sacerdotales dans le diocèse de Cambrai au XVIIIe siècle (1727-1788)
Authors: Deregnaucourt Gilles 
Published: October 2015

The Cambrai diocese offered in the xvinth century a rate of recruiting to the ecclesiastical function approaching 3 clergymen for any thousand inhabitants over periods of 25 years, that is largely over the French average (between one and two per thousand). This widespread sacerdotal vocation can be accounted for by, all at once, a strong demographic growth, a high degree of literacy, the system of secondary education, the efficiency of the diocesan seminary, the benefits system and, more largely, the impact of Counter- Reformation in the Netherlands.
Moreover, Cambrai did not experience the vocational crisis observed anywhere else after 1760 ; only a slight decline can be noticed here. In the same manner, it does not look as though there had been any real decline among the regular clergy.
This inquiry bases itself on the bishops' records of ordinations and ecclesiastical insinuations.
Fitness consequences of short‐ and long‐distance pollinations in Phlox hirsuta, an endangered species
Authors: Ruane Lauren G. , Dickens Morgan E. , Wall Morgan E. 
Published: October 2015

PREMISE OF THE STUDY:The persistence of rare and endangered plant species may depend on the distance pollinators travel when dispersing pollen. Pollinations between adjacent plants, which are often genetically similar, can decrease seed set, germination, and/or progeny vigor due to shared S‐alleles or inbreeding depression. Interpopulation pollen dispersal is often suggested as a management tool to increase genetic diversity; however, long‐distance pollinations also have the potential to decrease fitness.METHODS:We performed experimental hand pollinations in the field and germination experiments in a growth chamber to determine the effect of intrapopulation pollination distance (1 m, 10 m, and 100 m) on seed set, seed germination, progeny growth, and progeny reproduction in Phlox hirsuta. In addition, we included interpopulation pollinations (6740 m) to determine whether artificial gene flow is a viable management option for this endangered species.KEY RESULTS:Although pollination distance did not affect the number of healthy seeds produced or the likelihood of radicle emergence, it did significantly affect the ability of germinating seeds to successfully produce cotyledons. Outbreeding depression was observed during seed germination and early seedling development. Seedlings resulting from interpopulation pollinations developed more slowly and were less likely to survive to produce cotyledons than seedlings resulting from all three intrapopulation pollination distances.CONCLUSIONS:Our results suggest that the success of P. hirsuta does not depend on the distance pollinators travel within populations and that conservation strategies that involve transporting genes between populations can be counterproductive.
Dispositif ITEP et scolarisation
Authors: AIRe Collectif association , scolarisation Commission 
Published: October 2015

Le dispositif itep , dans la mesure où il se doit de favoriser autant que possible le « hors les murs » dans tous les types d’interventions, appelle à une évolution, voire une transformation, des représentations et des pratiques professionnelles. Ce processus de changement d’ordre culturel nécessite temps, attention, ainsi qu’une organisation souple et modulable.	Cette organisation permet de combiner et d’articuler différents lieux de scolarisation possibles, en interne ou à l’extérieur, auprès d’écoles et d’établissements de proximité partenaires ainsi que les établissements de référence... La notion traditionnelle de « classe » a entre autres tendance à s’effacer au bénéfice de celle de « modules d’inclusion » selon des modalités très diversifiées et évolutives.	L’unité d’enseignement ( ue ) de l’ itep ne peut en aucun cas remplacer l’école ou le collège de référence. Elle constitue une étape dans un parcours global de scolarisation lui-même inscrit dans un processus à visée soignante.
L’habillement est issu des liens familiaux
Authors: Berquière Thierry 
Published: October 2015

Le vêtement est un objet de représentation sociale et un système de signes distinctifs déterminant des différences de génération, de sexe, de culture. L’habillement a aussi une fonction importante dans les liens primordiaux, qui participe de la construction des enveloppes psychiques. L’auteur recherche comment l’in-vestiture , qui marque des différences structurantes, et l’in-vestissement , qui qualifie ces liens, vont tisser une trame des liens familiaux, dans laquelle le corps est inscrit. Des récits fondateurs, et tout particulièrement celui de Perceval , nous indiquent comment le vêtement est un passeur qui accompagne les séparations. La présentation d’une thérapie familiale montre comment à partir d’une peau commune déchirée, l’enveloppe vestimentaire a permis de tisser des liens entre des « traces sans mémoire » et faire une place au transgénérationnel.
Statut du corps et du symptôme obésité dans l’économie psychique familiale au travers du dessin de la maison imaginaire
Authors: Schwailbold Marie-Anne , Cuynet Patrice 
Published: October 2015

Les auteurs présentent l’évolution de la prise en charge d’une famille à problématique d’obésité. À travers l’étude de l’enveloppe corporelle groupale, ils proposent une compréhension de la dynamique familiale. Ils utilisent pour cela un médiateur groupal projectif, à savoir l’épreuve du dessin de la maison imaginaire, « spatiographie projective ». À travers cet outil ils investiguent la manière dont la famille projette son « nous familial » sur l’espace feuille. Le dessin rend alors compte de la représentation inconsciente du corps familial.