bluuebunny/crossref_metadata_2025_split huggingface dataset:
The temperature and pressure dependence of the dynamic properties of branched perfluoropolyethers
Authors: Bair Scott 
Published: November 2013

The relative volume and the limiting low shear viscosity have been accurately determined as functions of temperature and pressure for a series of eight branched perfluoropolyether liquids. The Murnaghan equation of state was fitted to the volumes and three correlations for viscosity were examined: the Doolittle free volume, the Bair and Casalini thermodynamic scaling, and the improved Yasutomi. The ambient-pressure bulk modulus increases gradually with molecular weight and is low compared with conventional lubricants. The Doolittle parameter increases with molecular mass as does the occupied volume fraction. The Ashurst–Hoover interaction parameter decreases with increasing molecular mass. The improved Yasutomi model provides accuracy comparable to models requiring an equation of state. When the kinematic viscosity at 40℃ is greater than about 200 mm2/s, fragility is independent of pressure. When the kinematic viscosity is less than about 200 mm2/s fragility increases with pressure. The pressure counterpart to the Angell plot and to the Vogel, Tammann, and Fulcher equation suggested by Drozd-Rzoska et al. is shown to be workable using stability limit pressures in the tensile domain. Clearly, progress in quantitative elastohydrodynamic lubrication must rely upon accurate measurements of the dynamic properties as a function of temperature and pressure and upon accurate temperature and pressure correlations for these properties. Only then can precise predictions of film thickness and friction be made.
Increased expression of ADAMTS13 mRNA correlates with ischemic cerebrovascular disease in systemic lupus erythematosus patients
Authors: López De Padilla Consuelo M , Hein Molly S , Crowson Cynthia S , Choo Christopher S , Green Abigail B , Petri Michelle , Bilgic Hatice , Baechler Emily C , Reed Ann M 
Published: November 2013

Objective: We investigated ADAMTS13 (a disintegrin-like and metalloprotease with thrombospondin type 1 motif, member 13) messenger RNA levels as a biomarker of disease features in systemic lupus erythematosus. Methods: We measured and compared messenger RNA (mRNA) levels of ADAMTS13 in peripheral blood cells in patients with systemic lupus erythematosus and healthy control subjects by whole-genome microarray. We retrospectively analyzed the correlations of ADAMTS13 mRNA expression with clinical features, laboratory parameters, therapeutic features, and disease activity (according to the Systemic Lupus Erythematosus Disease Activity Index). We also examined the association of three single nucleotide polymorphisms (rs4962145, rs2285467, and rs685523) of the ADAMTS13 gene with patient characteristics. Results: In 309 patients, the median ADAMTS13 mRNA expression levels were significantly higher in blood cells of systemic lupus erythematosus patients than in 23 healthy controls ( p = .03). Notably, ADAMTS13 mRNA expression levels were significantly higher in systemic lupus erythematosus patients with a history of stroke ( p = .02) or transient ischemic attack ( p = .02). Among the three single nucleotide polymorphisms analyzed, rs2285467 was significantly associated with stroke ( p = .03) and anticardiolipin antibodies ( p = .04). Conclusions: Increased expression of ADAMTS13 mRNA in blood cells is associated with the presence of ischemic cerebrovascular disease in systemic lupus erythematosus patients and suggests a potential role for ADAMTS13 in the pathogenesis of ischemic cerebrovascular disease in these patients.
Interstitial lung disease associated with Equine Infectious Anemia Virus infection in horses
Authors: Bolfa Pompei , Nolf Marie , Cadoré Jean-Luc , Catoi Cornel , Archer Fabienne , Dolmazon Christine , Mornex Jean-François , Leroux Caroline 
Published: December 2013

AbstractEIA (Equine Infectious Anemia) is a blood-borne disease primarily transmitted by haematophagous insects or needle punctures. Other routes of transmission have been poorly explored. We evaluated the potential of EIAV (Equine Infectious Anemia Virus) to induce pulmonary lesions in naturally infected equids. Lungs from 77 EIAV seropositive horses have been collected in Romania and France. Three types of lesions have been scored on paraffin-embedded lungs: lymphocyte infiltration, bronchiolar inflammation, and thickness of the alveolar septa. Expression of the p26 EIAV capsid (CA) protein has been evaluated by immunostaining. Compared to EIAV-negative horses, 52% of the EIAV-positive horses displayed a mild inflammation around the bronchioles, 22% had a moderate inflammation with inflammatory cells inside the wall and epithelial bronchiolar hyperplasia and 6.5% had a moderate to severe inflammation, with destruction of the bronchiolar epithelium and accumulation of smooth muscle cells within the pulmonary parenchyma. Changes in the thickness of the alveolar septa were also present. Expression of EIAV capsid has been evidenced in macrophages, endothelial as well as in alveolar and bronchiolar epithelial cells, as determined by their morphology and localization. To summarize, we found lesions of interstitial lung disease similar to that observed during other lentiviral infections such as FIV in cats, SRLV in sheep and goats or HIV in children. The presence of EIAV capsid in lung epithelial cells suggests that EIAV might be responsible for the broncho-interstitial damages observed.
Construction of high-density genetic linkage maps for orange-spotted grouper Epinephelus coioidesusing multiplexed shotgun genotyping
Authors: You Xinxin , Shu Liping , Li Shuisheng , Chen Jieming , Luo Jian , Lu Jun , Mu Qian , Bai Jie , Xia Qiuju , Chen Qingchun , Cai Yanjie , Zhang Haifa , Chen Guohua , Lin Haoran , Zhang Yong , Shi Qiong 
Published: December 2013

Abstract

Background
Orange-spotted grouper, Epinephelus coioides, is one of the most valuable fish species in China. Commercial production of orange-spotted grouper could be increased by developing higher growth rates and improving commercially important traits. Information on genetic markers associated with quantitative trait loci (QTL) can be used in breeding programs to identify and select individuals carrying desired traits. A high-density genetic linkage map is the basis for QTL study, and multiplexed shotgun genotyping (MSG) facilitates the development of single nucleotide polymorphisms (SNPs) and genotyping. In this study, the first high-density genetic linkage maps for groupers were generated on the basis of the MSG method.

Results
The sex-averaged map contained a total of 4,608 SNPs, which spanned 1581.7 cM, with a mean distance between SNPs of 0.34 cM. The 4,608 SNPs were located in 2,849 unique locations on the linkage map, with an average inter-location space at 0.56 cM. There were 2,516 SNPs on the female map, and the number of unique locus was 1,902. However, the male map contained more numbers of SNP (2,939) and unique locations (2,005). The total length of the female and male maps was 1,370.9 and 1,335.5 cM, respectively.

Conclusions
The high-resolution genetic linkage maps will be very useful for QTL analyses and marker-assisted selection (MAS) for economically important traits in molecular breeding of the orange-spotted grouper.
Comparative transcriptome analysis of tomato (Solanum lycopersicum) in response to exogenous abscisic acid
Authors: Wang Yan , Tao Xiang , Tang Xiao-Mei , Xiao Liang , Sun Jiao-long , Yan Xue-Feng , Li Dan , Deng Hong-Yuan , Ma Xin-Rong 
Published: December 2013

AbstractBackgroundAbscisic acid (ABA) can regulate the expressions of many stress-responsive genes in plants. However, in defense responses to pathogens, mounting evidence suggests that ABA plays variable roles. Little information exists about genome-wide gene expression in ABA responses in tomato (Solanum lycopersicumL.), a model fruit crop plant.ResultsGlobal transcriptome profiles of tomato leaf responses to exogenous ABA were generated using Illumina RNA-sequencing. More than 173 million base pair reads were mapped onto the tomato reference genome and the expression pattern differences between treated and control leaves were assessed. In total, 50,616 transcripts were generated. Among them, 42,583 were functionally annotated in the NCBI non-redundant database and 47,877 in the tomato genome reference. Additionally, 31,107 transcripts were categorized into 57 functional groups based on Gene Ontology terms, and 14,371 were assigned to 310 Kyoto Encyclopedia of Genes and Genomes pathways. In both the ABA treatment and control samples, 39,671 transcripts were available to analyze their expressions, of which 21,712 (54.73%) responded to exogenous ABA. Of these transcripts, 2,787 were significantly differently expressed genes (DEGs). Many known and novel ABA-induced and -repressed genes were found. Exogenous ABA can influence the ABA signaling pathway with PYR/PYL/RCARs-PP2Cs-SnRK2s as the center. Eighteen PYL genes were detected. A large number of genes related to various transcription factors, heat shock proteins, pathogen resistance, and the salicylic acid, jasmonic acid, and ethylene signaling pathways were up-regulated by exogenous ABA.ConclusionsThe results indicated that ABA has the potential to improve pathogen-resistance and abiotic stress tolerance in tomato. This study presents the global expression analysis of ABA-regulated transcripts in tomato and provides a robust database for investigating the functions of genes induced by ABA.
Development and characterization of microsatellite markers for Morus spp. and assessment of their transferability to other closely related species
Authors: Mathithumilan Balachandran , Kadam Niteen Narharirao , Biradar Jyoti , Reddy Sowmya H , Ankaiah Mahadeva , Narayanan Madhura J , Makarla Udayakumar , Khurana Paramjit , Sreeman Sheshshayee Madavalam 
Published: December 2013

Abstract

Background
Adoption of genomics based breeding has emerged as a promising approach for achieving comprehensive crop improvement. Such an approach is more relevant in the case of perennial species like mulberry. However, unavailability of genomic resources of co-dominant marker systems has been the major constraint for adopting molecular breeding to achieve genetic enhancement of Mulberry. The goal of this study was to develop and characterize a large number of locus specific genic and genomic SSR markers which can be effectively used for molecular characterization of mulberry species/genotypes.

Result
We analyzed a total of 3485 DNA sequences including genomic and expressed sequences (ESTs) of mulberry (Morus alba L.) genome. We identified 358 sequences to develop appropriate microsatellite primer pairs representing 222 genomic and 136 EST regions. Primers amplifying locus specific regions of Dudia white (a genotype of Morus alba L), were identified and 137 genomic and 51 genic SSR markers were standardized. A two pronged strategy was adopted to assess the applicability of these SSR markers using mulberry species and genotypes along with a few closely related species belonging to the family Moraceae viz., Ficus, Fig and Jackfruit. While 100% of these markers amplified specific loci on the mulberry genome, 79% were transferable to other related species indicating the robustness of these markers and the potential they hold in analyzing the molecular and genetic diversity among mulberry germplasm as well as other related species. The inherent ability of these markers in detecting heterozygosity combined with a high average polymorphic information content (PIC) of 0.559 ranging between 0.076 and 0.943 clearly demonstrates their potential as genomic resources in diversity analysis. The dissimilarity coefficient determined based on Neighbor joining method, revealed that the markers were successful in segregating the mulberry species, genotypes and other related species into distinct clusters.

Conclusion
We report a total of 188 genomic and genic SSR markers in Morus alba L. A large proportion of these markers (164) were polymorphic both among mulberry species and genotypes. A substantial number of these markers (149) were also transferable to other related species like Ficus, Fig and Jackfruit. The extent of polymorphism revealed and the ability to detect heterozygosity among the cross pollinated mulberry species and genotypes render these markers an invaluable genomic resource that can be utilized in assessing molecular diversity as well as in QTL mapping and subsequently mulberry crop improvement through MAS.
Body image and eating patterns among adolescents
Authors: Bibiloni Maria del Mar , Pich Jordi , Pons Antoni , Tur Josep A 
Published: December 2013

Abstract

Background
Data on the association between body self-perception and eating patterns among adolescents are scarce. This study assessed the association between body image and eating patterns among normal-weight, overweight and obese adolescents.

Methods
A cross-sectional survey (n = 1231; 12–17 years old) was carried out in the Balearic Islands, Spain. Anthropometry, body image, socio-economic determinants, and food consumption were studied.

Results
Fifty-one percent of boys and sixty percent of girls that wished to be thinner had less than or equal to 3 eating occasions per day. Overfat girls that wish to be thinner skipped breakfast more frequently than normal-fat girls. Overfat boys and girls that wished a thinner body reported lower consumption of several food groups than normal-fat adolescents and overfat boys satisfied with their own body image (i.e. breakfast cereals, pasta and rice dishes, other oils and fats, high fat foods, soft drinks and chocolates in boys; and dairy products and chocolates in girls).A restriction of Western diet foods and energy intake was associated with a wish to be thinner among overfat adolescents.

Conclusions
Many overfat boys were satisfied with their body image while practically all overfat girls reported wishing a thinner body. Meal patterns and food consumption were associated with body dissatisfaction and overfat status among adolescents.
Invasively-treated incidence of lower extremity peripheral arterial disease and associated factors in Taiwan: 2000–2011 nationwide hospitalized data analysis
Authors: Chang Nien-Tzu , Chan Chien-Lung , Lu Yu-Tzuen , Hsu Jin-Chyr , Hsu Yuan-Nian , Chu Dachen , Yang Nan-Ping 
Published: December 2013

Abstract

Background
Lower extremity (LE) peripheral artery disease (PAD), which is associated with a reduced quality of life and increased mortality from atherosclerotic cardio-/cerebro-vascular occlusion, is a significant public health problem, especial for an aging society such as that of Taiwan.

Methods
Specific datasets of the 2000–2011 nationwide inpatient databases were analyzed. Two inclusion criteria, including one of the major diagnosis codes of PAD and one of three categorical invasive treatments of LE PAD, were used consecutively to select cases diagnosed as LE PAD and receiving invasive treatment. The epidemiology of invasively-treated PAD in Taiwan was estimated, and the influences of potential confounders on these invasively-treated methods were evaluated.

Results
In general, the invasively-treated incidence of PAD in Taiwan doubled, from 3.73/10,000 (in 2000) to 7.48/10,000 (in 2011). On average, the total direct medical cost of one hospitalized and invasively-treated PAD case ranged from $US 4,600 to $US 5,900. The annual cases of bypass surgery for the PAD cases averaged 1,000 and the cases for limb amputation ranged from 4,100 to 5,100 annually. However, the number of percutaneous transluminal angioplasty (PTA) procedures remarkably increased by 15 times, from 600/year to 9,100/year, from 2000 to 2011. 51.3% of all the enrolled cases were treated with limb amputations, and female, young and middle-aged people (30–65 years of age), DM patients and those on a low income had a tendency to undergo amputation due to PAD. 37.6% of all the enrolled cases were treated with PTAs related to hypertension, cardiovascular disease, hyperlipidemia and catastrophic Illness. 2-year PTA failure rates of 22.13%, 11.91% and 10.61% were noted among the first (2000–2001), second (2004–2005) and the third (2008–2009) cohort groups, respectively.

Conclusions
In Taiwan, a gender difference and age and period effects on the invasively-treated incidence of LE PAD were observed. Female, young and middle-aged people (30–50 and 50–65 years of age), DM patients and those on a low income had a tendency to undergo amputation. The number of PTA procedures remarkably increased, but the 2-year failure rate of PTAs reduced from 2000 to 2011.
Safety reporting on implantation of autologous adipose tissue-derived stem cells with platelet-rich plasma into human articular joints
Authors: Pak Jaewoo , Chang Jae-Jin , Lee Jung Hun , Lee Sang Hee 
Published: December 2013

Abstract

Background
Adipose tissue-derived stem cells (ADSCs), a type of mesenchymal stem cells (MSCs), have great potential as therapeutic agents in regenerative medicine. Numerous animal studies have documented the multipotency of ADSCs, showing their capabilities to differentiate into tissues such as muscle, bone, cartilage, and tendon. However, the safety of autologous ADSC injections into human joints is only beginning to be understood and the data are lacking.

Methods
Between 2009 and 2010, 91 patients were treated with autologous ADSCs with platelet-rich plasma (PRP) for various orthopedic conditions. Stem cells in the form of stromal vascular fraction (SVF) were injected with PRP into various joints (n = 100). All patients were followed for symptom improvement with visual analog score (VAS) at one month and three months. Approximately one third of the patients were followed up with third month magnetic resonance imaging (MRI) of the injected sites. All patients were followed up by telephone questionnaires every six months for up to 30 months.

Results
The mean follow-up time for all patients was 26.62 ± 0.32 months. The follow-up time for patients who were treated in 2009 and early 2010 was close to three years. The relative mean VAS of patients at the end of one month follow-up was 6.55 ± 0.32, and at the end of three months follow-up was 4.43 ± 0.41. Post-procedure MRIs performed on one third of the patients at three months failed to demonstrate any tumor formation at the implant sites. Further, no tumor formation was reported in telephone long-term follow-ups. However, swelling of injected joints was common and was thought to be associated with death of stem cells. Also, tenosinovitis and tendonitis in elderly patients, all of which were either self-limited or were remedied with simple therapeutic measures, were common as well.

Conclusions
Using both MRI tracking and telephone follow ups in 100 joints in 91 patients treated, no neoplastic complications were detected at any ADSC implantation sites. Based on our longitudinal cohort, the autologous and uncultured ADSCs/PRP therapy in the form of SVF could be considered to be safe when used as percutaneous local injections.
An unusual delayed complication of paraffin self-injection for penile girth augmentation
Authors: De Siati Mario , Selvaggio Oscar , Di Fino Giuseppe , Liuzzi Giuseppe , Massenio Paolo , Sanguedolce Francesca , Carrieri Giuseppe , Cormio Luigi 
Published: December 2013

Abstract

Background
Penile self-injection of various oils is still carried out among Eastern Europe people for penile girth augmentation despite the potential destructive complications of this practice are well known. Penile reactions to such foreign bodies include scarring, abscess formation, ulceration, and even Fournier’s gangrene; voiding problems due to mineral oil self-injection have been reported only once. To our knowledge, we describe the first case of paraffin self-injection for penile girth augmentation presenting with acute urinary retention.

Case presentation
A 27-year-old Romanian man presented with severe penile pain and acute urinary retention five years after having practiced repeated penile self-injections of paraffin for penile girth augmentation. The penile shaft was massively enlarged, fibrotic and phymotic; urethral catheterization failed due to severe stricture of the proximal pendulum urethra. The patients refused placement of a suprapubic catheter and underwent immediate penile surgical exploration. The scarred tissue between dartos and Buck’s fascia and a fibrotic ring occluding the urethra were removed and the penile skin reconstructed. Pathology confirmed the diagnosis of paraffinoma. The patient resumed normal voiding immediately after catheter removal on second postoperative day; he was very pleased with cosmetic, sexual and voiding results at six weeks, six months and 1 year follow-up.

Conclusions
The present report describes a novel complication of penile self-injection for penile girth augmentation. Because of the increasing number of patients seeking penile augmentation, physicians dealing with sexual medicine should pay more attention to such request to prevent the use of non medical treatments that can turn into medical disasters.
Severe hematuria after transurethral electrocoagulation in a patient with an arteriovesical fistula
Authors: Zheng Xiangyi , Lin Yiwei , Chen Bin , Zhou Xianyong , Zhou Xiaofeng , Shen Yuehong , Xie Liping 
Published: December 2013

AbstractBackgroundArteriovesical fistulas are extremely rare. Only eleven cases were previously reported in the literature. They can occur iatrogenically, traumatically or spontaneously.Case presentationWe report an unusual case of a 62-year-old woman with arteriovesical fistula that developed fatal hematuria after transurethral electrocoagulation. Computed tomography (CT) and selective angiography revealed a pseudoaneurysm of the right superior vesical artery with arteriovesical fistula formation, which was managed by transarterial embolization.ConclusionsContrast enhanced CT or CT angiography should be performed when a pulsatile hemorrhage is revealed during cystoscopy. Therapeutic vesical arterial embolization should be considered as a safe and effective procedure for arteriovesical fistulas. Transurethral electrocoagulation may cause severe hematuria for pulsatile bladder bleeding in patients with pelvic vascular malformation.