bluuebunny/crossref_metadata_2025_split huggingface dataset:
X-ray and neutron imaging for cultural heritage: the INFN-CHNet experience
Authors: Mazzinghi A. , Castelli L. , Ruberto C. , Barone S. , García-Avello Bofías F. , Bombini A. , Czelusniak C. , Gelli N. , Giambi F. , Manetti M. , Massi M. , Sodi L. , Balerna A. , Pronti L. , Romani M. , Angelucci M. , Viviani G. , Sciarra V. , Cestelli Guidi M. , Marabotto M. , Sottili L. , Vigorelli L. , Tansella F. , Magalini M. , Guidorzi L. , Re A. , Lo Giudice A. , Ricci C. , Pozzi F. , Iorio G. , Graziani V. , Fabbri A. , Branchini P. , De Carlo A. , Tortora L. , Morigi M. P. , Bettuzzi M. , Brancaccio R. , Seracini M. , Cantini F. , Grazzi F. , Marcucci G. , Clemenza M. , Alloni D. , Altieri S. , Rossini R. , Salvini A. , Sans-Planell O. , Zafiropoulos D. , Sarchiapone L. , Arneodo F. , Torres Saavedra R. A. , Castellá M. F. , Mastrangelo N. , Morales A. M. , Taccetti F. , Giuntini L. 
Published: July 2024

AbstractThis paper reports on the instrumentation and expertise developed within the INFN-CHNet network for X-ray and neutron imaging, which enable non-invasive identification of materials and production processes in the field of cultural heritage. INFN-CHNet is the network of the Italian National Institute of Nuclear Physics specifically dedicated to the development and application of scientific methods and technologies to cultural heritage. This article focuses on portable MA-XRF scanners, often complemented by additional techniques, PIXE imaging on a newly developed portable accelerator, X-ray radiography and tomography, exploited to their full potential also through the use of portable systems, and neutron radiography and tomography, which require large-scale facilities. In many respects, the information obtained from X-ray and neutron-based methods is complementary, facilitating a comprehensive characterisation of materials, structures, and manufacturing techniques.
Graphical abstract
Symmetry analysis, optimal system, conservation laws and exact solutions of time-fractional diffusion-type equation
Authors: Yu Jicheng , Feng Yuqiang 
Published: July 2024

In Liu et al. [On the generalized time fractional diffusion equation: Symmetry analysis, conservation laws, optimal system and exact solutions, Int. J. Geom. Methods Mod. Phys. 17(01) (2020) 2050013], the generalized time-fractional diffusion equation [Formula: see text] is studied by the symmetry analysis method. Liu et al. obtained two group generators [Formula: see text] [Formula: see text] and only one trivial solution [Formula: see text] for the equation. In this paper, we classify the Lie symmetry group admitted by the equation, and for the case [Formula: see text], we obtain a richer set of group generators and some nontrivial solutions, including unprecedented exact solutions and power series solutions. In addition, we construct the one-dimensional optimal system of the Lie symmetry group admitted by the time-fractional diffusion-type equation by Olver’s method, and obtain the conservation laws for all the obtained Lie symmetries using the general method developed by Ibragimov. For the novel exact solutions and power series solutions, we analyze their dynamic behavior graphically.
Electrochemical Detection of Arsenic Using Eco-Friendly Zeolite-Graphite Composite Electrode
Authors: Ait Baha Abdellah , Idouhli Rachid , Tabit Kamal , Kazemian Hossein , Khadiri Mohy Eddine , Abouelfida Abdesselam 
Published: July 2024

This paper presents an innovative and stable electrochemical sensor using zeolite and graphite for the reliable and accurate detection of arsenic(V). The zeolite materials were hydrothermally synthesized using coal fly ash and fumed silica by-products under specific environments (1.5 M NaOH, L/(Si/Al) ratio of 40, 120 °C, and 24 h), addressing environmental concerns related to wastes managment. The performance of the zeolite-graphite modified electrode (ZGME) was evaluated using voltammetric and impedance techniques at potentials between 1.2 V and −1.2 V (vs SCE) and a frequency range of 100 kHz to 10 mHz, respectively. The results showed that the incorporation of zeolite for the first time offered notable advantages, such as affordability, simplicity, and improved oxidation-reduction current and peak resolution for arsenic. The ZGME effectively detected As(V) under neutral conditions using a phosphate buffer solution, with a concentration range of 1 × 10−3 to 1 × 10−6 mol.l−1. The sensor achieved a detection limit of 3 μmol.l−1, a quantification limit of 5 μmol.l−1, a sensitivity of 0.28 μA/μM.cm2, and exhibited good reproducibility, opening up new potential for portable zeolite-based electrochemical sensors. Moreover, this research pioneers the use of zeolite as a graphite modifier.
MR1 Gene and Protein Expression Are Enhanced by Inhibition of the Extracellular Signal-Regulated Kinase ERK
Authors: Constantin Daniel , Nosi Vladimir , Kehrer Natalie , Vacchini Alessandro , Chancellor Andrew , Contassot Emmanuel , Beshirova Aisha , Prota Gennaro , Navarini Alexander , Mori Lucia , De Libero Gennaro 
Published: July 2024

Abstract
The MHC class I–related molecule MR1 is ubiquitously expressed, is highly conserved among mammals, and presents bacterial and endogenous antigens in tumor cells. These features indicate that tumor-specific T cells restricted to MR1 may represent ideal candidates for novel cancer-directed T-cell immunotherapy. The very low expression of the MR1 protein at the cell surface is a potential challenge limiting the possible use of MR1-directed immunotherapies. To overcome this challenge, it is important that understanding of the mechanisms regulating MR1 expression is increased, as little is known about this currently. This study identified ERK1/2 as negative regulators of the MR1 gene and protein expression. Inhibition of ERK1/2 in tumor cells or treatment of BRAF-mutant tumor cells with drugs specific for mutated BRAF increased MR1 protein expression and recognition by tumor-reactive and MR1-restricted T cells. The ERK1/2 inhibition of MR1 was mediated by the ELF1 transcription factor, which was required for MR1 gene expression. The effects of ERK1/2 inhibition also occurred in cancer cell lines of different tissue origins, cancer cell lines resistant to drugs that inhibit mutated BRAF, and primary cancer cells, making them potential targets of specific T cells. In contrast to tumor cells, the recognition of healthy cells was very poor or absent after ERK1/2 inhibition. These findings suggest a pharmaceutical approach to increase MR1 protein expression in tumor cells and the subsequent activation of MR1-restricted T cells, and they have potential therapeutic implications.
Role of Health Care Professionals in the Success of Blood Pressure Control Interventions in Patients With Hypertension: A Meta-Analysis
Authors: Mills Katherine T. , O’Connell Samantha S. , Pan Meng , Obst Katherine M. , He Hua , He Jiang 
Published: July 2024

BACKGROUND:
Globally, only 13.8% of patients with hypertension have their blood pressure (BP) controlled. Trials testing interventions to overcome barriers to BP control have produced mixed results. Type of health care professional delivering the intervention may play an important role in intervention success. The goal of this meta-analysis is to determine which health care professionals are most effective at delivering BP reduction interventions.

METHODS:
We searched Medline and Embase (until December 2023) for randomized controlled trials of interventions targeting barriers to hypertension control reporting who led intervention delivery. One hundred articles worldwide with 116 comparisons and 90 474 participants with hypertension were included. Trials were grouped by health care professional, and the effects of the intervention on systolic and diastolic BP were combined using random effects models and generalized estimating equations.

RESULTS:
Pharmacist-led interventions , community health worker–led interventions, and health educator–led interventions resulted in the greatest systolic BP reductions of −7.3 (95% CI, −9.1 to −5.6), −7.1 (95% CI, −10.8 to −3.4), and −5.2 (95% CI, −7.8 to −2.6) mm Hg, respectively. Interventions led by multiple health care professionals, nurses, and physicians also resulted in significant systolic BP reductions of −4.2 (95% CI, −6.1 to −2.4), −3.0 (95% CI, −4.2 to −1.9), and −2.4 (95% CI, −3.4 to −1.5) mm Hg, respectively. Similarly, the greatest diastolic BP reductions were −3.9 (95% CI, −5.2 to −2.5) mm Hg for pharmacist-led and −3.7 (95% CI, −6.6 to −0.8) mm Hg for community health worker–led interventions. In pairwise comparisons, pharmacist were significantly more effective than multiple health care professionals, nurses, and physicians at delivering interventions.

CONCLUSIONS:
Pharmacists and community health workers are most effective at leading BP intervention implementation and should be prioritized in future hypertension control efforts.
Action Interpretation Determines the Effects of Go/No-Go and Approach/Avoidance Actions on Stimulus Evaluation
Authors: Chen Zhang , Van Dessel Pieter 
Published: July 2024

Abstract
Executing go/no-go or approach/avoidance responses toward a stimulus can change its evaluation. To explain these effects, some theoretical accounts propose that executing these responses inherently triggers affective reactions (i.e., action execution), while others posit that the evaluative influences originate from interpreting these responses as valenced actions (i.e., action interpretation). To test the role of action execution and action interpretation in these evaluative effects, we developed a novel training task that combined both go/no-go and approach/avoidance actions orthogonally. Participants either responded or did not respond (i.e., go/no-go) to control a shopping cart on screen, and as a result, either collected or did not collect (i.e., approach/avoidance) certain food items. When the task instructions referred to the go/no-go actions (Experiment 1, N = 148), we observed an effect of these actions. Participants evaluated no-go items less positively than both go and untrained items. No effect of approach/avoidance actions was observed. Contrarily, when the task instructions referred to the approach/avoidance actions (Experiment 2, N = 158), we observed an approach/avoidance effect. Participants evaluated approached items more positively and avoided items less positively than untrained items. No effect of go/no-go actions was observed. This suggests that action interpretation determined whether go/no-go or approach/avoidance actions influenced stimulus evaluation, when the same motor responses were made. Further examination of the role of action interpretation can inform theories of how actions influence stimulus evaluation, and facilitate the use of these interventions in applied settings.
The Eternal Present: A Photovoice Study of the Experience of Geriatrics Residents During the COVID-19 Pandemic
Authors: Negrete‐Najar Juan Pablo , Radosh Sverdlin Adina , Arreola Rodríguez Alejandro , Navarrete Reyes Ana Patricia 
Published: July 2024

During the COVID-19 pandemic, medical residents had the task of being the frontline of the response, being exposed to high risk of infection, increased clinical duty, and long and irregular working hours in highly restricted environments, increasing their levels of stress. We sought to expose the experiences of a group of geriatrics residents during this period of change in their professional and personal lives through the photovoice methodology. Thirteen participants were recruited and had 2 weeks to take photographs. The photographs were discussed in group meetings; the content of the conversations was transcribed and analyzed using interpretive description. Sixteen themes were identified. They were divided into personal life (11 themes) and life as a resident (5 themes). Adaptation was the main theme that came into discussion. The photographs and themes show how life changed for the participants, having a feeling of isolation, especially from their families, and highlighting their experiences as a team and community. While the pandemic, particularly at its beginning, was a period of uncertainty and a heavy load of work, it also provided learning and experience to this group of young physicians, which should not hide the fact that mental health concerns and burnout were a common situation. An online gallery was created which is publicly accessible.
Validity and reliability of the functional test for hemiplegic upper Extremity–Thai version
Authors: Chinchai Pisak , Pingmuang Pheangrawee , Chinchai Krongporn , Kaunnil Anuchart 
Published: July 2024

Background There are very few standard instruments currently available for measuring upper extremity (UE) functions for patients with stroke in Thailand. Objectives This study aims to examine the concurrent validity, construct validity, and stability reliability of the Functional Test for Hemiplegic Upper Extremity (FTHUE)-Thai version for patients with stroke. Methods Thirty hemiplegic participants from five community rehabilitation centers in Chiang Mai province and 30 healthy subjects were recruited. The FTHUE-Thai version and the Fugl-Meyer Assessment for the Upper Extremity (FMA-UE) were the instruments used. Concurrent validity was determined by investigating the relationship between the FTHUE-Thai version and the FMA-UE. Construct validity was investigated by comparing the performance of FTHUE-Thai version between stroke participants and healthy subjects. The stability reliability of the FTHUE-Thai version, which measured the UE function of stroke participants twice in a two-week’s period, was also investigated. The statistics used were Spearman’s correlation coefficient and the Mann-Whitney test. Results There were significant correlations between the UE function, as measured by the FTHUE-Thai version, and the arm, and hand sub-scores, as well as the total scores of the FMA-UE ( r = 0. 93, r = 0. 84, and r = 0. 95, respectively), indicating good concurrent validity. Stability reliability was also good ( r = 0. 98, weighted kappa = 0. 94). A known group technique test revealed significantly different scores between stroke patients and healthy subjects ( p < . 001), indicating good construct validity. Conclusion The FTHUE-Thai version could be a reliable measurement tool for the UE function in stroke patients in the Thai context.
Monitoring correlates of SARS-CoV-2 infection in cell culture using a two-photon-active calcium-sensitive dye
Authors: Máthé Domokos , Szalay Gergely , Cseri Levente , Kis Zoltán , Pályi Bernadett , Földes Gábor , Kovács Noémi , Fülöp Anna , Szepesi Áron , Hajdrik Polett , Csomos Attila , Zsembery Ákos , Kádár Kristóf , Katona Gergely , Mucsi Zoltán , Rózsa Balázs József , Kovács Ervin 
Published: July 2024

Abstract
Background
The organism-wide effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral infection are well studied, but little is known about the dynamics of how the infection spreads in time among or within cells due to the scarcity of suitable high-resolution experimental systems. It has been reported that SARS-CoV-2 infection pathways converge at calcium influx and subcellular calcium distribution changes. Imaging combined with a proper staining technique is an effective tool for studying subcellular calcium-related infection and replication mechanisms at such resolutions.

Methods
Using two-photon (2P) fluorescence imaging with our novel Ca-selective dye, automated image analysis and clustering analysis were applied to reveal titer and variant effects on SARS-CoV-2-infected Vero E6 cells.

Results
The application of a new calcium sensor molecule is shown, combined with a high-end 2P technique for imaging and identifying the patterns associated with cellular infection damage within cells. Vero E6 cells infected with SARS-CoV-2 variants, D614G or B.1.1.7, exhibit elevated cytosolic calcium levels, allowing infection monitoring by tracking the cellular changes in calcium level by the internalized calcium sensor. The imaging provides valuable information on how the level and intracellular distribution of calcium are perturbed during the infection. Moreover, two-photon calcium sensing allowed the distinction of infections by two studied viral variants via cluster analysis of the image parameters. This approach will facilitate the study of cellular correlates of infection and their quantification depending on viral variants and viral load.

Conclusions
We propose a new two-photon microscopy-based method combined with a cell-internalized sensor to quantify the level of SARS-CoV-2 infection. We optimized the applied dye concentrations to not interfere with viral fusion and viral replication events. The presented method ensured the proper monitoring of viral infection, replication, and cell fate. It also enabled distinguishing intracellular details of cell damage, such as vacuole and apoptotic body formation. Using clustering analysis, 2P microscopy calcium fluorescence images were suitable to distinguish two different viral variants in cell cultures. Cellular harm levels read out by calcium imaging were quantitatively related to the initial viral multiplicity of infection numbers. Thus, 2P quantitative calcium imaging might be used as a correlate of infection or a correlate of activity in cellular antiviral studies.

Graphical Abstract
Transcriptome analysis suggested that lncRNAs regulate rapeseed seedlings in responding to drought stress by coordinating the phytohormone signal transduction pathways
Authors: Tan Xiaoyu , Long Weihua , Ma Ni , Sang Shifei , Cai Shanya 
Published: July 2024

AbstractThe growth, yield, and seed quality of rapeseed are negatively affected by drought stress. Therefore, it is of great value to understand the molecular mechanism behind this phenomenon. In a previous study, long non-coding RNAs (lncRNAs) were found to play a key role in the response of rapeseed seedlings to drought stress. However, many questions remained unanswered. This study was the first to investigate the expression profile of lncRNAs not only under control and drought treatment, but also under the rehydration treatment. A total of 381 differentially expressed lncRNA and 10,253 differentially expressed mRNAs were identified in the comparison between drought stress and control condition. In the transition from drought stress to rehydration, 477 differentially expressed lncRNAs and 12,543 differentially expressed mRNAs were detected. After identifying the differentially expressed (DE) lncRNAs, the comprehensive lncRNAs-engaged network with the co-expressed mRNAs in leaves under control, drought and rehydration was investigated. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of co-expressed mRNAs identified the most significant pathways related with plant hormones (expecially abscisic acid, auxin, cytokinins, and gibberellins) in the signal transduction. The genes, co-expressed with the most-enriched DE-lncRNAs, were considered as the most effective candidates in the water-loss and water-recovery processes, including protein phosphatase 2 C (PP2C), ABRE-binding factors (ABFs), and SMALL AUXIN UP-REGULATED RNAs (SAURs). In summary, these analyses clearly demonstrated that DE-lncRNAs can act as a regulatory hub in plant-water interaction by controlling phytohormone signaling pathways and provided an alternative way to explore the complex mechanisms of drought tolerance in rapeseed.
Adolescent gut microbiome imbalance and its association with immune response in inflammatory bowel diseases and obesity
Authors: Joo Minjae , Nam Seungyoon 
Published: July 2024

Abstract
Background
Recently, there has been an increase in the number of studies focusing on the association between the gut microbiome and obesity or inflammatory diseases, especially in adults. However, there is a lack of studies investigating the association between gut microbiome and gastrointestinal (GI) diseases in adolescents.

Method
We obtained 16S rRNA-seq datasets for gut microbiome analysis from 202 adolescents, comprising ulcerative colitis (UC), Crohn’s disease (CD), obesity (Ob), and healthy controls (HC). We utilized Quantitative Insights Into Microbial Ecology (QIIME) and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) to acquire Operational Taxonomic Units (OTUs). Subsequently, we analyzed Kyoto Encyclopedia of Genes and Genomes (KEGG) Orthology (KO) terms and pathway enrichment for the identified OTUs.

Results
In this study, we investigated the difference between the gut microbiomes in adolescents with GI diseases and those in healthy adolescents using 202 samples of 16S rRNA sequencing data. The distribution of the six main gut microbiota (i.e., unclassified Dorea, unclassified Lachnospiraceae, unclassified Ruminococcus, Faecalibacterium prausnitzii, Prevotella copri, unclassified Sutterella) was different based on the status of obesity and inflammatory diseases. Dysbiosis was observed within Lachnospiraceae in adolescents with inflammatory diseases (i.e., UC and CD), and in adolescents with obesity within Prevotella and Sutterella. More specifically, our results showed that the relative abundance of Faecalibacterium prausnitzii and unclassified Lachnospiraceae was more than 10% and 8% higher, respectively, in the UC group compared to the CD, Ob, and HC groups. Additionally, the Ob group had over 20% and over 3% higher levels of Prevotella copri and unclassified Sutterella, respectively, compared to the UC, CD, and HC groups. Also, inspecting associations between the six specific microbiota and KO terms, we found that the six microbiota -relating KO terms were associated with NOD-like receptor signaling. These six taxa differences may affect the immune system and inflammatory response by affecting NOD-like receptor signaling in the host during critical adolescence.

Conclusion
In this study, we discovered that dysbiosis of the microbial community had varying degrees of influence on the inflammatory and immune response pathways in adolescents with inflammatory diseases and obesity.
Morphological characterization and transcriptome analysis of rolled and narrow leaf mutant in soybean
Authors: Xu Xiaomin , Wang Yongzhen , Lu Housheng , Zhao Xueqian , Jiang Jiacan , Liu Mengshi , Yang Cunyi 
Published: July 2024

Abstract
Background
In plants, the leaf functions as a solar panel, where photosynthesis converts carbon dioxide and water into carbohydrates and oxygen. In soybean, leaf type traits, including leaf shape, leaf area, leaf width, and leaf width so on, are considered to be associated with yield. In this study, we performed morphological characterization, transcriptome analysis, and endogenous hormone analysis of a rolled and narrow leaf mutant line (rl) in soybean.

Results
Compared with wild type HX3, mutant line rl showed rolled and narrower leaflet, and smaller leaf, meanwhile rl also performed narrower pod and narrower seed. Anatomical analysis of leaflet demonstrated that cell area of upper epidermis was bigger than the cell area of lower epidermis in rl, which may lead rolled and narrow leaf. Transcriptome analysis revealed that several cytokinin oxidase/dehydrogenase (CKX) genes (Glyma.06G028900, Glyma.09G225400, Glyma.13G104700, Glyma.14G099000, and Glyma.17G054500) were up-regulation dramatically, which may cause lower cytokinin level in rl. Endogenous hormone analysis verified that cytokinin content of rl was lower. Hormone treatment results indicated that 6-BA rescued rolled leaf enough, rescued partly narrow leaf. And after 6-BA treatment, the cell area was similar between upper epidermis and lower epidermis in rl. Although IAA content and ABA content were reduced in rl, but exogenous IAA and ABA didn’t affect leaf type of HX3 and rl.

Conclusions
Our results suggest abnormal cytokinin metabolism caused rolled and narrow leaf in rl, and provide valuable clues for further understanding the mechanisms underlying leaf development in soybean.