bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of C1R promotes hepatocellular carcinoma development by activating HIF‐1α‐regulated glycolysis
Authors: Ma Yuying , Wang Yuehua , Tuo Peng , Meng Zhongji , Jiang Bin , Yuan Yahong , Ding Yan , Naeem Abid , Guo Xingrong , Wang Xiaoli 
Published: August 2024

AbstractC1R has been identified to have a distinct function in cutaneous squamous cell carcinoma that goes beyond its role in the complement system. However, it is currently unknown whether C1R is involved in the progression of hepatocellular carcinoma (HCC). HCC tissues were used to examine C1R expression in relation to clinical and pathological factors. Malignant characteristics of HCC cells were assessed through in vitro and in vivo experiments. The mechanism underlying the role of C1R in HCC was explored through RNA‐seq, methylation‐specific PCR, immuno‐precipitation, and dual‐luciferase reporter assays. This study found that the expression of C1R decreased as the malignancy of HCC increased and was associated with poor prognosis. C1R promoter was highly methylated through DNMT1 and DNMT3a, resulting in a decrease in C1R expression. Downregulation of C1R expression resulted in heightened malignant characteristics of HCC cells through the activation of HIF‐1α‐mediated glycolysis. Additionally, decreased C1R expression was found to promote xenograft tumor formation. We found that C‐reactive protein (CRP) binds to C1R, and the free CRP activates the NF‐κB signaling pathway, which in turn boosts the expression of HIF‐1α. This increase in HIF‐1α leads to higher glycolysis levels, ultimately promoting aggressive behavior in HCC. Methylation of the C1R promoter region results in the downregulation of C1R expression in HCC. C1R inhibits aggressive behavior in HCC in vitro and in vivo by inhibiting HIF‐1α‐regulated glycolysis. These findings indicate that C1R acts as a tumor suppressor gene during HCC progression, opening up new possibilities for innovative therapeutic approaches.
Oscillatory hypoxia induced gene expression predicts low survival in human breast cancer patients
Authors: Suhail Yasir , Liu Yamin , Du Wenqiang , Afzal Junaid , Qiu Xihua , Atiq Amina , Vera‐Licona Paola , Agmon Eran , Kshitiz  
Published: August 2024

AbstractHypoxia is one of the key factors in the tumor microenvironment regulating nearly all steps in the metastatic cascade in many cancers, including in breast cancer. The hypoxic regions can however be dynamic with the availability of oxygen fluctuating or oscillating. The canonical response to hypoxia is relayed by transcription factor Hypoxia‐Inducible Factor 1 (HIF‐1), which is stabilized in hypoxia and acts as the master regulator of a large number of downstream genes. However, HIF‐1 transcriptional activity can also fluctuate either due to unstable hypoxia, or by lactate mediated noncanonical degradation of HIF‐1. Our understanding of how oscillatory hypoxia or HIF‐1 activity specifically influences cancer malignancy is very limited. Here, using MDA‐MB‐231 cells as a model of triple negative breast cancer characterized by severe hypoxia, we measured the gene expression changes induced specifically by oscillatory hypoxia. We found that oscillatory hypoxia can specifically regulate gene expression differently, and at times opposite to stable hypoxia. Using the Cancer Genome Atlas RNAseq data of human cancer samples, we show that the oscillatory specific gene expression signature in MDA‐MB‐231 is enriched in most human cancers, and prognosticates low survival in breast cancer patients. In particular, we found that oscillatory hypoxia, unlike stable hypoxia, induces unfolded protein folding response in cells resulting in gene expression predicting reduced survival.
Enhancing mechanical and tribological performance of hybrid composites: An experimental study utilizing response surface methodology and firefly algorithm
Authors: Dash Soumya , Satpathy Mantra Prasad , Routara Bharat Chandra , Pati Pravat Ranjan , Gantayat Subhra 
Published: August 2024

AbstractThis study emphasizes the significance of stacking sequence and hybridization of glass, carbon, kevlar and basalt fibers to enhance the mechanical characteristics and the overall wear response of polymer composites. The carbon layer on the outside of the composite exhibited higher ultimate tensile and flexural strengths. The abrasive wear of fabricated hybrid composites is also explored by performing experiments using Box–Behnken design approach. The pin‐on‐disc tester is utilized to do the wear test by varying composite type, sliding distance, and sliding velocity, with specific wear rate (SWR) serving as the response parameter. Regression analysis is performed to predict SWR using control and response parameters derived from experimentation. A novel firefly algorithm technique is adopted to determine the optimal process parameter combination. By utilizing optimized parameters (430 m, 10.5 m/s, and the CKBG4BKC stacking sequence), the SWR is considerably reduced to 16.82 × 10−5 mm3/Nm. Scanning electron microscopy on the worn‐out wear surface reveals enhanced interfacial bonding, fiber breakage and plowing as the fundamental wear mechanism. This work provides insight into hybrid composites for constructing aircraft and automobile body structures, where they provide an optimal blend of strength, sustainability, and structural performance.Highlights
Hybrid composite: Stacking sequence impacts on mechanical and abrasive wear.
Box–Behnken design: Applied on stacking order, sliding distance and velocity.
Utilizing metaheuristic firefly algorithm to enhance specific wear results.
Optimal parameters: 430 m, 10.5 m/s, and CKBG4BKC stacking sequence.
Lightweight, high‐strength, cost‐effective, and sustainable hybrid composites.
A Bimetallic Nanomodulator to Reverse Immunosuppression via Sonodynamic‐Ferroptosis and Lactate Metabolism Modulation
Authors: Deng Xi , Zhu Yutong , Dai Zideng , Liu Qing , Song Ze , Liu Tianzhi , Huang Yuefeng , Chen Hangrong 
Published: August 2024

AbstractTriple‐negative breast cancer (TNBC) responds poorly to immunotherapy due to insufficient immunogenicity and highly immunosuppressive tumor microenvironment (TME). Herein, an intelligent calcium/cobalt‐based nanomodulator (Ca,Co)CO3‐LND‐TCPP@F127‐TA (abbreviated as CCLT@FT) is developed to act as a sonodynamic‐ferroptosis inducer and metabolic immunoadjuvant to enhance anti‐tumor immunotherapy. More details, simultaneous reactive oxygen species (ROS) generation and glutathione (GSH) depletion can be achieved due to the existence of Co2+/Co3+ redox couple in CCLT@FT. Meanwhile, mitochondrial Ca2+ overload and tetrakis(4‐carboxyphenyl) porphyrin (TCPP)‐mediated sonodynamic therapy (SDT) further amplify the oxidative stress and promote ferroptosis in tumor cells. More impressively, CCLT@FT can modulate lactate metabolism by doping with cobalt and loading with lonidamine (LND, an inhibitor of MCT4), thereby reversing the high‐lactate immunosuppressive TME. Furthermore, the combination with immune checkpoint blockade (ICB) therapy is found to achieve superior anti‐tumor immunity, which in turn promotes ferroptosis of tumor cells by downregulating SLC7A11 protein, ultimately creating a “cycle” therapy. Overall, this work demonstrates a novel strategy for enhancing anti‐tumor immunotherapy based on a closed‐loop enhancement therapeutic route between CCLT@FT inducing ferroptosis/lactate metabolism modulation and ICB therapy, providing an alternative and important reference for effective immunotherapy of TNBC.
Multifunctional Bone Regeneration Membrane with Flexibility, Electrical Stimulation Activity and Osteoinductive Activity
Authors: Zhang Shihao , Huang Lei , Bian Mengxuan , Xiao Lan , Zhou Dong , Tao Ziwei , Zhao Zheng , Zhang Jian , Jiang Li‐Bo , Li Yulin 
Published: August 2024

AbstractThe use of membrane‐based guided bone regeneration techniques has great potential for single‐stage reconstruction of critical‐sized bone defects. Here, a multifunctional bone regeneration membrane combining flexible elasticity, electrical stimulation (ES) and osteoinductive activity is developed by in situ doping of MXene 2D nanomaterials with conductive functionality and β‐TCP particles into a Poly(lactic acid‐carbonate (PDT) composite nano‐absorbable membrane (P/T/MXene) via electrostatic spinning technique. The composite membrane has good feasibility due to its temperature sensitivity, elastic memory capacity, coordinated degradation profile and easy preparation process. In vitro experiments showed the P/T/MXene membrane effectively promoted the recruitment and osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) under ES and enhanced the angiogenic capacity of endothelial cells, which synergistically promoted bone regeneration through neovascularization. In addition, an in vivo rat model of cranial bone defects further confirmed the bone regeneration efficacy of the P/T/MXene membrane. In conclusion, the developed P/T/MXene membrane can effectively promote bone regeneration through their synergistic multifunctional effects, suggesting the membranes have great potential for guiding tissue regeneration and providing guidance for the biomaterials design.
Amyand's hernia: Cause of inguinal swelling
Authors: Vasin Dragan , Vukovic Goran , Mijovic Ksenija , Pavlovic Aleksandar , Miskovic Bojana , Maricic Bojana , Doklestic Krstina , Masulovic Dragan 
Published: August 2024

AbstractAmyand's hernia is a condition where the vermiform appendix is contained within an inguinal hernia sac. An inguinal hernia with an appendix in the hernia sac is extremely rare and almost exclusively occurs on the right side. The mechanism of developing appendicitis with hernia is most likely the compression of the appendix at the level of the neck of the sac or due to impaired vascular supply leading to infection. Despite technological advances in radiology modalities, the preoperative diagnosis of and hernia is challenging: the role of abdominal ultrasound and computed tomography is very important and is essential in planning surgical management and approaches. In our case, we made a rare preoperative diagnosis of Amyand's hernia using ultrasound showing a typical appearance only without wall hyperemia probably due to vascular compression of the inguinal ring, which was confirmed by CT. CT is more sensitive to the detection of radiological signs of appendiceal inflammation and perforation in the hernia sac and is most often used in the case of an inconclusive ultrasound findings. Due to the prompt radiological diagnosis of acute appendicitis in the hernia sac provided by ultrasound, our patient underwent laparoscopic appendectomy.
Extraction, Modification of Radix Paeoniae Alba Starch and Adsorption Application
Authors: Liu Xueli , Wang Lanlan , Zhu Chunfeng 
Published: August 2024

AbstractRadix Paeoniae Alba is an important plant resource and valuable tonic in traditional Chinese medicine. The radix of Radix Paeoniae Alba is rich in starch. There are few reports of Radix Paeoniae Alba starch. In present study, the Radix Paeoniae Alba starch is extracted, citrate esterified starches are prepared, the starch‐based hydrogels are synthesized, and adsorptive properties are investigated. The investigation suggests that Radix Paeoniae Alba starch‐based adsorbent is promising as an efficient and sustainable adsorbent for copper ions and methylene blue removal; therefore, Radix Paeoniae Alba starch has an appealing application prospect in adsorption for scavenging metal ions and dyes from real complex waste liquid.
Uterine intussusception in a pet rat (Rattus norvegicus)
Authors: Berry Alexandra , Sladakovic Izidora 
Published: August 2024

AbstractUterine intussusception is defined as the uterus telescoping onto itself. This condition has been rarely described in animals, and when it occurs, is often associated with post‐partum or pregnant animals. This case report describes the diagnosis and treatment of a uterine horn intussusception in a 1‐year 7‐month‐old, female, entire nulliparous pet rat (Rattus norvegicus). The rat presented for a 1‐day history of sanguineous vaginal discharge. An exploratory laparotomy was performed, and the rat was diagnosed with a firm, off‐white approximately 1 cm cervical mass and a left uterine horn intussusception. An ovariohysterectomy was performed for therapeutic and diagnostic purposes, and the mass was diagnosed as a benign endometrial stromal polyp on histopathology. Ten days post‐operative, the surgical incision developed a bacterial infection, which resolved with a course of amoxicillin/clavulanic acid. No further post‐operative complications occurred. The rat continues to do well 10 months after surgery.
Fluoride geochemistry in groundwater at regulated industrial sites
Authors: Fowler Andrew , Goulding Nigel , Law Shanna 
Published: August 2024

AbstractFew studies apply geochemical concepts governing fluoride fate and transport in natural waters to geochemical conditions at contaminated industrial sites. This has negative implications for designing sampling and compliance monitoring programs and informing remediation decision‐making. We compiled geochemical data for 566 groundwater samples from industrial waste streams associated with elevated fluoride and that span a range of geochemical conditions, including alkaline spent potliner, near‐neutral pH coal combustion, and acidic gypsum stack impoundments. Like natural systems, elevated fluoride (hundreds to thousands of ppm) exists at the pH extremes and is generally tens of ppm at near‐neutral pH conditions. Geochemical models identify pH‐dependent fluoride complexation at low pH and carbonate stability at high pH as dominant processes controlling fluoride mobility. Limitations in available thermochemical, kinetic rate, and adsorption/desorption data and lack of complete analyses present uncertainties in quantitative models used to assess fluoride mobility at industrial sites.Practitioner Points
Geochemical fundamentals of fluoride fate and transport in groundwater are communicated for environmental practitioners.
Fluoride is a reactive constituent in groundwater, and factors that govern attenuation are identified.
Geochemical models are useful for identifying fluoride attenuation processes, but quantitative use is limited by thermodynamic data uncertainties.
Thermal process computing in nanofluid using dissipation and squeezing constraints
Authors: Mishra Nidhish Kumar , Adnan  , Ashraf Waqas , Mir Ahmed , Kolsi Lioua 
Published: August 2024

AbstractThe investigation of thermal mechanisms in traditional as well as advanced fluid phases grabbed huge attention of the engineers around the globe. These fluids have a bright future in numerous fields including but not restricted to chemical, applied thermal, and heat transfer applications. Therefore, the present analysis emphasized on the development and investigation of a new nanofluid model under physical constraints like viscous dissipation, squeezing, and nanoparticle concentration effects. The fourth‐order heat transfer model is formulated using new thermophysical properties of tetra nanofluids and similar transformative functions. Then, the model was analyzed numerically and a deep discussion of the results was provided. It is noticed that the motion reduced near the bottom wall rapidly when the tetra nanoparticles concentration is taken from to and inward the sheet movement enhanced it. The fluid motion can be augmented by accelerating the top sheet in an outward direction by keeping . Moreover, the shear drag increased in the range of to and the heat transfer rate enhanced from to and to when and increased.
Residues of TRPM8 at the Lipid-Water-Interface have Coevolved with Cholesterol Interaction and are Relevant for Diverse Health Disorders
Authors: Shikha Deep , Dalai Ritesh , Kumar Shamit , Goswami Chandan 
Published: August 2024

AbstractTRPM8 is a non-selective cation channel that is expressed in several tissues and cells and also has a unique property to be activated by low-temperature. In this work, we have analyzed the conservation of amino acids that are present in the lipid-water-interface (LWI) region of TRPM8, the region which experiences a microenvironment near the membrane surface. We demonstrate that the amino acids present in the LWI region are more conserved than the transmembrane or even full-length TRPM8, suggesting strong selection pressure in these residues. TRPM8 also has several conserved cholesterol-binding motifs where cholesterol can bind in different modes and energies. We suggest that mutations and/or physiological conditions can potentially alter these TRPM8-cholesterol complexes and can lead to physiological disorders or even apparently irreversible diseases such as cancer and neurodegeneration.
Graphical abstract
Diagnostic performance of liver stiffness as marker of liver involvement in systemic immunoglobulin light chain (AL) amyloidosis
Authors: Brunger Anne F. , Tingen Hendrea S.A. , Bijzet Johan , van Rheenen Ronald , Blokzijl Hans , Roeloffzen Wilfried W. H. , Houwerzijl Ewout J. , Muntinghe Friso L. H. , Slart Riemer H. J. A. , Gans Reinold O. B. , Kimmich Christoph , Hazenberg Bouke P. C. , Nienhuis Hans L. A. 
Published: August 2024

AbstractObjective: To investigate the diagnostic performance of liver stiffness for detecting liver involvement in immunoglobulin light chain (AL) amyloidosis. Methods: Liver stiffness was measured using transient elastography in 71 patients with systemic AL amyloidosis and 18 patients with wild type transthyretin (ATTRwt) amyloidosis with cardiomyopathy. Both non-invasive consensus criteria and serum amyloid P component (SAP) scintigraphy were used as substitute standards instead of liver biopsy for establishing liver involvement. Results: Liver stiffness was higher in AL amyloidosis patients with liver involvement than in those without: this was observed using both consensus criteria (median 14.4 kPa vs. 8.1 kPa;p = 0.001) and SAP scintigraphy (median 20.9 kPa vs. 6.2 kPa;p < 0.001). Liver stiffness was also higher in AL amyloidosis patients with liver involvement compared to AL and ATTRwt amyloidosis patients with cardiac involvement. Based on receiver operating characteristic (ROC) curves a cut-off value of 14.4 kPa for stiffness was optimal to indicate liver involvement, providing sensitivity and specificity of 50% and 74%, respectively, using the consensus criteria and 63% and 90%, respectively, using SAP scintigraphy as standard. Conclusion: Liver stiffness is a promising tool to establish liver involvement in AL amyloidosis having potential to become part of updated criteria for liver involvement.
Phenotypic variability of Montastraea cavernosa and Porites astreoides along a depth gradient from shallow to mesophotic reefs in the Cayman Islands
Authors: Doherty Matthew L. , Chequer Alex D. , Mass Tali , Goodbody-Gringley Gretchen 
Published: August 2024

AbstractPhenotypic variability is the ability of the same species to express different phenotypes under different environmental conditions. Several coral species that exist along a broad depth distribution have been shown to differ in skeletal morphology and nutrient acquisition at different depths, which has been attributed to variability in response to differing levels of light availability. This study examined the phenotypic variability of two common depth generalist corals, Montastraea cavernosa and Porites astreoides, along a gradient from 10 to 50 m in the Cayman Islands, by examining changes in skeletal morphology, photophysiology, symbiont cell density, and chlorophyll concentration. Skeletal features of M. cavernosa were found to increase in size from 10 to 30 m, but returned to smaller sizes from 30 to 50 m, while P. astreoides skeletal features continued to increase in size from 10 to 40 m. No differences were observed in either symbiont density or chlorophyll concentration across depths for either species. However, all photophysiological parameters exhibited significant depth-dependent variations in both species, revealing adaptive strategies to different light environments. These results suggest that both species have high variability in response to depth. Patterns of skeletal morphology and photophysiology, however, suggest that M. cavernosa may be more variable in regulating photosynthetic efficiency compared to P. astreoides, which likely facilitates the broader depth distribution of this species.
Priorisierte Terminvergabe bei Neuvorstellungen: Was ist wirklich entscheidend?
Authors: Krämer Stefan , Flöge A. , Handt S. , Juzek-Küpper F. , Vogt K. , Ullmann J. , Rauen T. 
Published: August 2024

Zusammenfassung
Hintergrund
Die zeitnahe Terminvergabe für Neuvorstellungen ist eine tägliche Herausforderung in der rheumatologischen Praxis, die von digitalen Lösungen unterstützt werden kann. Es stellt sich die Frage nach einer möglichst einfachen und effektiven Methode der Terminpriorisierung.

Methoden
Mithilfe eines Anmeldeformulars für Neuvorstellungen wurden standardisiert Symptome und Laborbefunde erfasst. Die Terminvergabe erfolgte nach fachärztlicher Sichtung dieser Informationen in 3 Kategorien: (a) < 6 Wochen, (b) 6 Wochen bis 3 Monate und (c) > 3 Monate. Die Wartezeiten zwischen dem Zeitpunkt der Anmeldung und dem Vorstellungstermin wurden berechnet und verglichen zwischen Patienten mit und ohne Diagnose einer entzündlich-rheumatischen Erkrankung (ERE). Zusätzlich wurde ein Entscheidungsbaum, eine Methode aus dem Bereich des überwachten Lernens innerhalb der künstlichen Intelligenz (KI), erstellt und die resultierende Klassifikation bezüglich Trefferrate und berechneter Wartezeitersparnis verglichen.

Ergebnisse
Insgesamt wurden 800 Fälle (darunter 555 Frauen [69,4 %], medianes Alter 53 Jahre [IQA 39–63]) zwischen 2020 und 2023 ausgewertet. Eine ERE konnte in 409 (51,1 %) Fällen bestätigt werden mit einer Wartezeit von 58 vs. 93 Tagen bei Non-ERE-Fällen (−38 %, p < 0,01). Eine KI-Stratifizierung ergab eine Trefferrate von 67 % bezüglich einer ERE und eine prognostizierte Einsparung von 19 % Wartezeit. Die Trefferrate stieg hierbei auf 78 % mit einer Zeitersparnis für ERE-Fälle um bis zu 31 %, wenn grundlegende Laborergebnisse bekannt waren. Andererseits ergaben vereinfachte Algorithmen z. B. durch eine reine Laborwert-basierte Stratifizierung eine niedrigere Trefferrate und Zeitersparnis.

Schlussfolgerung
Die fachärztliche Terminzuweisung ist effektiv und verkürzt die Terminwartezeit für Patienten mit ERE signifikant. Eine automatisierte Kategorisierung kann unter Berücksichtigung vollständiger Laborwerte mit reduzierter Sensitivität zu einer Verkürzung der Terminwartezeit führen.
Prediction of neonatal outcomes using gestational age vs ACOG definitions of maternal disease severity in hypertensive disorders of pregnancy
Authors: Hauptman Isabella , Gill Kevin S. , Lim Tiffany , Mack Wendy J. , Wilson Melissa L. 
Published: August 2024

Abstract
Purpose
Hypertensive disorders of pregnancy cause significant neonatal complications. Disease severity is often used to predict neonatal outcomes, however gestational age (GA) at delivery may be a better predictor. We aimed to assess whether disease severity or GA was more predictive of adverse neonatal outcomes.

Methods
We included 165 participants with confirmed HELLP syndrome or severe preeclampsia (sPE). Two predictive models were constructed to assess the ability of disease severity compared to GA to predict a composite adverse neonatal outcome. The composite outcome included low birth weight, SGA, IUGR, Apgar score, and neonatal death.

Results
Using severity as a predictor of binary neonatal outcome had an AUC of 0.73 (0.65–0.81), with a sensitivity (SE) of 70.3% and a specificity (SP) of 64.4%. For GA, we observed an AUC of 0.82 (0.75–0.89), with a SE of 75.7% and a SP of 76.7%.

Conclusion
For the composite neonatal outcome, GA was a better predictor than ACOG diagnosis (severity). This observation underscores the need for further research to validate these findings in larger cohorts and to determine their applicability to maternal outcomes.