bluuebunny/crossref_metadata_2025_split huggingface dataset:
Light‐Dependent Circadian Rhythm Governs O‐GlcNAc Cycling to Influence Cognitive Function in Adult Zebrafish
Authors: Park Jiwon , Kim Dong Yeol , Oh Eok‐Soo , Han Inn‐Oc 
Published: August 2024

ABSTRACTThis study explores the 24‐h rhythmic cycle of protein O‐GlcNAcylation within the brain and highlights its crucial role in regulating the circadian cycle and neuronal function based on zebrafish as an animal model. In our experiments, disruption of the circadian rhythm, achieved through inversion of the light‐dark cycle or daytime melatonin treatment, not only impaired the rhythmic changes of O‐GlcNAcylation along with altering expression patterns of O‐GlcNAc transferase (OGT) and O‐GlcNAcase (OGA) in zebrafish brain but also significantly impeded learning and memory function. In particular, circadian disruption affected rhythmic expression of protein O‐GlcNAcylation and OGT in the nuclear fraction. Notably, the circadian cycle induces rhythmic alterations in O‐GlcNAcylation of H2B histone protein that correspond to changes in H3 trimethylation. Disruption of the cycle interfered with these periodic histone code alterations. Pharmacological inhibition of OGT with OSMI‐1 disrupted the wake‐sleep patterns of zebrafish without affecting expression of circadian rhythm‐regulating genes. OSMI‐1 inhibited the expression of c‐fos, bdnf, and calm1, key genes associated with brain function and synaptic plasticity, and decreased the binding of O‐GlcNAcylated H2B and OGT to promoter regions of these genes. The collective findings support the potential involvement of circadian cycling of the O‐GlcNAc histone code in regulating synaptic plasticity and brain function. Overall, data from this study provide evidence that protein O‐GlcNAcylation serves as a pivotal posttranslational mechanism integrating circadian signals and neuronal function to regulate rhythmic physiology.
Sources of reduced visual acuity and spectacle treatment options for individuals with Down syndrome: Review of current literature
Authors: Anderson Heather A. 
Published: August 2024

AbstractIndividuals with Down syndrome are known to have a greater prevalence of ocular conditions such as strabismus, nystagmus, elevated refractive error, poor accommodative function, elevated higher‐order optical aberrations and corneal abnormalities. Related to these conditions, individuals with Down syndrome commonly have reduced best‐corrected visual acuity at both far and near viewing distances across their lifespan. This review summarises the various optical sources of visual acuity reduction in this population and describes clinical trials that have evaluated alternative spectacle prescribing strategies to minimise these optical deficits. Although refractive corrections may still have limitations in their ability to normalise visual acuity for individuals with Down syndrome, the current literature provides evidence for eye care practitioners to consider in their prescribing practices for this population to maximise visual acuity. These considerations include accounting for the presence of elevated higher‐order aberrations when determining refractive corrections and considering bifocal lens prescriptions, even for young children with Down syndrome.
Workload of home care nurses: Italian adaptation, validity, and reliability of the National Aeronautics and Space Administration Task Load Index questionnaire
Authors: Zaghini Francesco , Calzolari Michela , Di Nitto Marco , Longobucco Yari , Alvaro Rosaria , Cicolini Giancarlo , Lancia Loreto , Manara Duilio Fiorenzo , Rasero Laura , Rocco Gennaro , Zega Maurizio , Mazzoleni Beatrice , Sasso Loredana , Bagnasco Annamaria 
Published: August 2024

AbstractThe aim of this study was to adapt the National Aeronautics and Space Administration Task Load Index (NASA‐TLX) to the home care setting and translate and validate it in Italian.An online questionnaire containing the Italian version of the NASA‐TLX adapted to the home care setting was administered to home care nurses to measure workload. Content Validity Index, Exploratory, and Confirmatory Factor Analyses were used to measure the psychometric characteristics of the modified NASA‐TLX.The modified Italian version of NASA‐TLX_HC‐IT showed good psychometric characteristics in measuring the workload of home care nurses, with excellent fit indices. The reliability, calculated with Cronbach's alpha, was 0.73, indicating adequate reliability. A negative correlation between workload and job satisfaction among home care nurses, as well as a positive association between high workload and intention to leave the workplace, was verified.The modified Italian version of the NASA‐TLX_HC‐IT was confirmed to be a valid and reliable instrument to measure workload in home care nursing. Furthermore, the correlation between workload and the intention to leave the workplace among home care nurses was an important result that community nursing managers should consider preventing the shortage of home care nurses.
Measures and factors for regularly and effectively holding case conferences to improve quality of care provided by public health nurses for individuals and families: A qualitative descriptive study
Authors: Sato Taichi , Ishimaru Mina 
Published: August 2024

AbstractObjectiveThis study clarified the measures and necessary factors for regularly and effectively conducting case conferences to improve the quality of public health nurses’ care for individuals and families.DesignWe employed a qualitative descriptive design.SampleThe study fields were three Japanese municipalities that regularly held case conferences for at least a year to improve the quality of public health nurses’ care for individuals and families. The first author conducted semi‐structured interviews with three lead public health nurses and two nurses responsible for case conference projects.MeasurementsThe audio recordings of the interviews were transcribed verbatim and categorized. Interviews were conducted between December 2021 and May 2022.ResultsMeasures to regularly and effectively conduct case conferences included “creating a system to promote case conferences,” “preparations to achieve case conference objectives,” and “case conference progress management.” Factors included “resources for promoting case conferences in the organization,” “public health nurses’ attitudes toward care,” and “an environment that allows discussions about care.”ConclusionLead public health nurses can use the results as a reference to implement the identified measures in their organizations. The factors can enable lead public health nurses to evaluate the status of their organization regarding conducting case conferences.
Global dynamics of a two‐stage structured diffusive population model in time‐periodic and spatially heterogeneous environments
Authors: Gueguezo H. M. , Doumatè T. J. , Salako R. B. 
Published: August 2024

AbstractThis work examines the global dynamics of classical solutions of a two‐stage (juvenile–adult) reaction–diffusion population model in time‐periodic and spatially heterogeneous environments. It is shown that the sign of the principal eigenvalue of the time‐periodic linearized system at the trivial solution completely determines the persistence of the species. Moreover, when , there is at least one time‐periodic positive entire solution. A fairly general sufficient condition ensuring the uniqueness and global stability of the positive time‐periodic solution is obtained. In particular, classical solutions eventually stabilize at the unique time‐periodic positive solutions if either each subgroup's intrastage growth and interstage competition rates are proportional, or the environment is temporally homogeneous and both subgroups diffuse slowly. In the latter scenario, the asymptotic profile of steady states with respect to small diffusion rates is established.
Nonlinear effects of uncertainty shocks: State dependency and asymmetry
Authors: Morita Hiroshi , Yuasa Shiro 
Published: August 2024

AbstractThe nonlinear effects of uncertainty shocks on U.S. macroeconomy are examined using a smooth transition VAR model in which the dynamic relationship between the variables changes with the level of economic policy uncertainty. Our results show that the nonlinear effects of uncertainty shocks depend on the current level of uncertainty, with uncertainty shocks behaving more like stronger demand shocks in the high‐uncertainty period than in the low period. Hence, we can conclude that the negative demand shock channel of uncertainty shocks, documented in the literature, is valid especially in the high‐uncertainty period.
Effects of the Presidential Awards for Excellence in Mathematics and Science Teaching (PAEMST) on teacher leadership trajectories
Authors: Ives Jillian , Falk Joni 
Published: August 2024

AbstractProviding teachers opportunities to lead has a positive effect on student achievement and teacher retention, which is critical in a time when there is a shortage of qualified STEM teachers. While there is research on the impact of professional development (PD) programs on teacher leadership, there is sparse literature on the impact of award or recognition programs such as the Presidential Awards for Excellence in Mathematics and Science Teaching (PAEMST). In this qualitative case study of eight PAEMST teacher leaders, we found that the PAEMST program accelerates and promotes STEM teacher leadership trajectories. The PAEMST program does this through: (a) the application process, which provided teachers with an informal PD experience; (b) the award notification experience, which served as external validation to boost teachers' confidence and identification as leaders; and (c) external validation and an expanded professional network, all of which led to increased leadership opportunities at the state and national level. Furthermore, we found increased opportunities to lead within the school and district was mediated by the reaction of their colleagues, school culture, and school leadership structures. We offer recommendations for administrators and practitioners in providing teacher leadership trajectories for awardees to positively influence their schools.
Development of a Novel Retinal Surgery Robot Based on Spatial Variable Remote Center-of-Motion Mechanism
Authors: Zhao Xiaolu , Wang Fujun , Tao Changping , Shi Beichao , Huo Zhichen , Tian Yanling 
Published: August 2024

Abstract
This article reports the design, modeling, and experiments of a novel retinal surgery robot based on spatial variable remote center-of-motion (RCM) mechanism. The general design criteria for parallel mechanisms are proposed, and the planar five-bar mechanisms are evaluated and selected. The planar-spatial evolution process, including the parallel connection of the planar mechanism and the equivalent substitution of joints, is adopted to develop a spatial variable RCM mechanism and then the robot. The mobility and singularity of the robot are analyzed, and the forward/inverse kinematics and workspace are modeled. Dimension optimization is conducted based on a comprehensive performance indicator that characterizes the motion range of linear actuators and the global dexterity performance index of robot. The prototyped robot is fabricated and assembled, and the kinematic calibration is performed. The position error of end-effector is within 34 μm, and both the position error and deviation of the RCM point are within 23 μm. The robot is demonstrated to reach the desired position and execute the RCM motion with high precision simultaneously.
Thermodynamic Optimization of a Combined Cooling and Power System Utilizing Industrial Waste Heat
Authors: Yin Ge , Yuan Tianhao , Li Deming , Zhang Chengbin , Deng Zilong 
Published: August 2024

Abstract
Cascade utilization is the most promising solution for efficient recovery of industrial waste heat. In this paper, a combined cooling and power (CCP) system including an organic Rankine cycle (ORC) and an absorption refrigeration cycle (ARC) is proposed, aiming to realize the cascade utilization of efficient utilization of low-grade flue gas. With the objective of maximizing the equivalent specific net power output, a particle swarm optimization (PSO) algorithm is employed to optimize the operation parameters of the proposed system and the composition and mass fractions of zeotropic working fluid. The results indicate that the proposed system can realize efficient cascade utilization for flue gas waste heat energy. The optimized proposed system achieves a specific net power output of 6.09 kW/kgFG of a specific refrigeration capacity of 27.65 kW/kgFG. Additionally, heat exchanger exergy losses account for 73.7% and 53.6% of the total exergy losses in the ORC and ABR subsystems, respectively. Therefore, optimizing the heat exchanger equipment is a feasible approach to further enhance the thermodynamic performance of the proposed system.
The robustness of porin-cytochrome gene clusters from
Geobacter metallireducens
in extracellular electron transfer
Authors: Zhuo Shiyan , Jiang Yongguang , Qi Lei , Hu Yidan , Jiang Zhou , Dong Yiran , Shi Liang 
Published: August 2024

ABSTRACT

To investigate their roles in extracellular electron transfer (EET), the porin-cytochrome (
pcc
) gene clusters
Gmet0825-0828
,
Gmet0908-0910
, and
Gmet0911-0913
of the Gram-negative bacterium
Geobacter metallireducens
were deleted. Failure to delete all
pcc
gene clusters at the same time suggested their essential roles in extracellular reduction of Fe(III)-citrate by
G. metallireducens
. Deletion of
Gmet0825-0828
had no impact on bacterial reduction of Fe(III)-citrate but diminished bacterial reduction of ferrihydrite and abolished anode reduction and direct interspecies electron transfer (DIET) to
Methanosarcina barkeri
and
Geobacter sulfurreducens
. Although it had no impact on the bacterial reduction of Fe(III)-citrate, deletion of
Gmet0908-0910
delayed ferrihydrite reduction, abolished anode reduction, and diminished DIET. Deletion of
Gmet0911-0913
had little impact on DIET but diminished bacterial reductions of Fe(III)-citrate, ferrihydrite, and anodes. Most importantly, deletions of both
Gmet0825-0828
and
Gmet0908-0910
restored bacterial reduction of ferrihydrite and anodes and DIET. Enhanced expression of
Gmet0911-0913
in this double mutant when grown in coculture with
G. sulfurreducens
Δ
hybL
Δ
fdnG
suggested that this cluster might compensate for impaired EET functions of deleting
Gmet0825-0828
and
Gmet0908-0910
. Thus, these
pcc
gene clusters played essential, distinct, overlapping, and compensatory roles in EET of
G. metallireducens
that are difficult to characterize as deletion of some clusters affected expression of others. The robustness of these
pcc
gene clusters enabled
G. metallireducens
to mediate EET to different acceptors for anaerobic growth even when two of its three
pcc
gene clusters were inactivated by mutation. The results from this investigation provide new insights into the roles of
pcc
gene clusters in bacterial EET.

IMPORTANCE

The Gram-negative bacterium
Geobacter metallireducens
is of environmental and biotechnological significance. Crucial to the unique physiology of
G. metallireducens
is its extracellular electron transfer (EET) capability. This investigation sheds new light on the robust roles of the three porin-cytochrome (
pcc
) gene clusters, which are directly involved in EET across the bacterial outer membrane, in the EET of
G. metallireducens
. In addition to their essential roles, these gene clusters also play distinct, overlapping, and compensatory roles in the EET of
G. metallireducens
. The distinct roles of the
pcc
gene clusters enable
G. metallireducens
to mediate EET to a diverse group of electron acceptors for anaerobic respirations. The overlapping and compensatory roles of the
pcc
gene clusters enable
G. metallireducens
to maintain and restore its EET capability for anaerobic growth when one or two of its three
pcc
gene clusters are deleted from the genome.
Molecular basis for sortase-catalyzed pilus tip assembly
Authors: Bhat Aadil H. , Chang Chungyu , Das Asis , Ton-That Hung 
Published: August 2024

ABSTRACT

During pilus assembly within the Gram-positive bacterial envelope, membrane-bound sortase enzymes sequentially crosslink specific pilus protein monomers through their cell wall sorting signals (CWSS), starting with a designated tip pilin, followed by the shaft made of another pilin, ultimately anchoring the fiber base pilin to the cell wall. To date, the molecular determinants that govern pilus tip assembly and the underlying mechanism remain unknown. Here, we addressed this in the model organism
Actinomyces oris
. This oral microbe assembles a pathogenically important pilus (known as type 2 fimbria) whose shafts, made of FimA pilins, display one of two alternate tip pilins—FimB or the coaggregation factor CafA—that share a markedly similar CWSS. We demonstrate that swapping the CWSS of CafA with that of FimB produces a functional hybrid, which localizes at the pilus tip and mediates polymicrobial coaggregation, whereas alanine-substitution of the conserved FLIAG motif within the CWSS hampers these processes. Remarkably, swapping the CWSS of the normal cell wall-anchored glycoprotein GspA with that of CafA promotes the assembly of hybrid GspA at the FimA pilus tip. Finally, exchanging the CWSS of the
Corynebacterium diphtheriae
shaft pilin SpaA with that of CafA leads to the FLIAG motif-dependent localization of the heterologous pilus protein SpaA at the FimA pilus tip in
A. oris
. Evidently, the CWSS and the FLIAG motif of CafA are both necessary and sufficient for its destination to the cognate pilus tip specifically assembled by a designated sortase in the organism.

IMPORTANCE

Gram-positive pili, whose precursors harbor a cell wall sorting signal (CWSS) needed for sortase-mediated pilus assembly, typically comprise a pilus shaft and a tip adhesin. How a pilin becomes a pilus tip, nevertheless, remains undetermined. We demonstrate here in
Actinomyces oris
that the CWSS of the tip pilin CafA is necessary and sufficient to promote pilus tip assembly, and this functional assembly involves a conserved FLIAG motif within the CWSS. This is evidenced by the fact that an
A. oris
cell-wall anchored glycoprotein, GspA, or a heterologous shaft pilin from
Corynebacterium diphtheriae
, SpaA, engineered to have the CWSS of CafA in place of their CWSS, localizes at the pilus tip in a process that requires the FLIAG motif. Our findings provide the molecular basis for sortase-catalyzed pilus tip assembly that is very likely employed by other Gram-positive bacteria and potential bioengineering applications to display antigens at controlled surface distance.
Endovascular treatment of unruptured intracranial aneurysms: Rate of thromboembolic events depicted by diffusion-weighted imaging in relation to different techniques
Authors: Pierot Laurent , Rouchaud Aymeric , Chabert Emmanuel , Desal Hubert , Ricolfi Frédéric , Barbe Coralie , Soize Sébastien , Zerroug Adberrahim , Eugène Francois , Clarençon Frédéric , Ferré Jean-Christophe 
Published: August 2024

BackgroundThe rate of thromboembolic events (TEEs) associated with endovascular treatment (EVT) of intracranial aneurysms is not reported uniformly in the literature due to the various ways that are used to evaluate them. Analysis of Thromboembolic Complications after Endovascular Treatment of Unruptured Intracranial Aneurysms study (ACET) is a prospective, multicenter study, which analyzes the rate of TEEs using diffusion-weighted imaging (DWI) magnetic resonance imaging (MRI) in patients treated for unruptured aneurysms with different endovascular techniques.MethodsPatients were prospectively included in six French centers. Postoperative DWI-MRI was performed within 72 hours post-procedure and independently evaluated. Univariate and multivariate analyses were conducted to determine factors associated with the occurrence of DWI lesions.ResultsOf the 233 included patients (54.5±11.2 years, 162 women, (69.5%)), 226 were effectively treated by EVT (coiling: 90 patients, 39.8%; balloon-assisted coiling (BAC): 62, 27.4%; stent-assisted coiling (SAC): 10, 4.4%; flow diversion (FD): 21, 9.3%; intrasaccular flow disruption (ISFD): 43, 19.0%) and had a postoperative MRI showing DWI lesions in 133 patients (58.8%). Univariate and multivariate analyses show the rate of patients with DWI lesions to be significantly higher with BAC (75.8%, P=0.001), SAC (90.0%, P=0.02), and FD (95.2%, P=0.001) compared with coiling alone (41.1%).ConclusionsThe rate of DWI lesions after EVT of unruptured aneurysms is primarily influenced by the EVT technique used. Techniques using transient (BAC) or permanent (SAC and FD) device placement in the parent artery are associated with a higher rate of DWI lesions.Trial registration numberACET: Unique identifier:NCT02862756.
Modeling and Sensitivity Estimation of a Dual Cavity Source Pocket-Based Charge Plasma Tunneling FET for Label-Free Biological Molecule Detection
Authors: Panda Shwetapadma , Dash Sidhartha 
Published: August 2024

A dual-source cavity charge plasma tunneling FET (DSC-SP-CPTFET) with SiGe Pocket is proposed, and its effectiveness as a biological sensor for label-free detection is explored. The fabrication complexity and cost have been reduced by using the charge-plasma concept. For improved sensing, an etched nanocavity is added to the upper and lower of the source metal section. The high-k (HfO2) gate oxide and minimal energy gap (Si0.6Ge0.4) alloy with a 40% mole fraction improve the current sensitivity by enhancing the drain current gradient. The sensitivity of the suggested biological sensor is assessed here for several neutral biological molecules, such as Gelatin, Keratin, Biotin, and 3-Aminopropyl-Triethoxysilane (APTES). Deoxyribonucleic acid (DNA), a charged biological molecule, is also considered with varying positive and negative charge densities. The suggested biological sensor shows a (SIDS)max of 2.21 × 1010 and a Sratio of 3.11 × 109 for biological molecules with higher dielectric constant at room temperature. Different electrostatic performances are estimated in the ON state, including energy band, electron (e-) BTBT rate, electrical field, and IDS-VGS characteristics. In addition, the proposed biological sensor provides a much superior drain current sensitivity (SIDS), current ratio sensitivity (Sratio), and average SS sensitivity (SSS) performance in the presence of both charged and neutral biological molecules.
Oncogenic Enhancers in Leukemia
Authors: Mulet-Lazaro Roger , Delwel Ruud 
Published: August 2024

Abstract
Although the study of leukemogenesis has traditionally focused on protein-coding genes, the role of enhancer dysregulation is becoming increasingly recognized. The advent of high-throughput sequencing, together with a better understanding of enhancer biology, has revealed how various genetic and epigenetic lesions produce oncogenic enhancers that drive transformation. These aberrations include translocations that lead to enhancer hijacking, point mutations that modulate enhancer activity, and copy number alterations that modify enhancer dosage. In this review, we describe these mechanisms in the context of leukemia and discuss potential therapeutic avenues to target these regulatory elements.
Significance: Large-scale sequencing projects have uncovered recurrent gene mutations in leukemia, but the picture remains incomplete: some patients harbor no such aberrations, whereas others carry only a few that are insufficient to bring about transformation on their own. One of the missing pieces is enhancer dysfunction, which only recently has emerged as a critical driver of leukemogenesis. Knowledge of the various mechanisms of enhancer dysregulation is thus key for a complete understanding of leukemia and its causes, as well as the development of targeted therapies in the era of precision medicine.