bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mono‐industrialism and the Struggle for Alternative Development: the Case of the Roşia Montană Gold‐mining Project
Authors: Vesalon Lucian , Creţan Remus 
Published: June 2013

AbstractThis study proposes a critical discussion of a recent gold‐mining project at Roşia Montană, Romania and of the alternative development solutions for the area. The aim of the research is to examine the socio‐economic consequences of the mining project proposed by the multinational company Roşia Montană Gold Corporation and the struggle of the resistance movement to the mining project for an alternative development, in order to understand the complexity of development processes in rural communities in post‐communist Romania. In analysing the socio‐economic risks involved in conventional development, we focus especially on revealing the intrinsic limits of mono‐industrialism and on the issue of population displacement by development. In the second part of the paper we discuss the feasibility of alternative development solutions for the area. The paper relies on a theoretical framework that combines the critical literature on conventional development and the recent debates on grassroots development.
Influence of antiviral therapy in the long‐term outcome of recurrent hepatitis C virus infection following liver transplantation
Authors: García‐Reyne A. , Lumbreras C. , Fernández I. , Colina F. , Abradelo M. , Magan P. , San‐Juan R. , Manrique A. , López‐Medrano F. , Fuertes A. , Lizasoain M. , Moreno E. , Aguado J.M. 
Published: June 2013

AbstractIntroductionSeverity of recurrent hepatitis C virus (HCV) infection in liver transplant recipients (LTR) is variable and the influence of different factors, including the administration of antiviral therapy in the long‐term outcome is controversial.MethodsWe analyzed the outcome of a cohort of HCV‐infected LTR who were transplanted in our institution. Patients were divided into 2 groups (severe and non‐severe HCV disease) depending on the presence of a fibrosis score of F ≥2 in the Scheuer index and/or fibrosing cholestasic hepatitis (FCH) in a graft biopsy. Risk factors were studied using logistic regression analysis. Survival of patients was estimated using Kaplan–Meier plots. A total of 146 patients were followed for a mean of 58 months.ResultsFifty‐six (34%) patients developed severe HCV disease and showed shorter survival (P < 0.024). Donor age (odds ratio [OR]: 1.04; 95% confidence interval [CI]: 1.02–1.06) and pre‐transplant viral load (VL) >106 UI/mL (OR: 3.5; 95% CI: 1.42–10.61) were the only factors associated with severe HCV infection. Over‐immunosuppression (OR: 2.3; 95% CI: 1.2–4.41) was specifically associated with the development of FCH. Overall, patient survival in recipients who received a full course of anti‐HCV therapy was higher than in patients who did not complete antiviral therapy (P = 0.004) or received no treatment (P = 0.007). Patients with non‐severe HCV infection have a higher probability of receiving a full course of antiviral therapy (P = 0.033).ConclusionIn conclusion, donor age, pre‐transplant VL, and over‐immunosuppression were associated with the long‐term development of severe HCV recurrence in liver grafts. Administration of a full course of antiviral therapy was associated with better survival.
Extremal Sasakian geometry on and related manifolds
Authors: Boyer Charles P. , Tønnesen-Friedman Christina W. 
Published: June 2013

AbstractWe prove the existence of extremal Sasakian structures occurring on a countably infinite number of distinct contact structures on${T}^{2} \times {S}^{3} $and certain related 5-manifolds. These structures occur in bouquets and exhaust the Sasaki cones in all except one case in which there are no extremal metrics.
Driving on Renewables—On the Prospects of Alternative Fuels up to
2050 From an Energetic Point-of-View in European Union Countries
Authors: Ajanovic Amela , Jungmeier Gerfried , Beermann Martin , Haas Reinhard 
Published: June 2013

Abstract
The core objective of this paper is to investigate the perspectives of “renewable fuels” mainly from an energetic point-of-view in a dynamic framework until 2050 in comparison to fossil fuels. In addition, the impact on the economic prospects of an improvement of the energetic performance is analyzed. As renewable fuels, various categories of first and second generation biofuels as well as electricity and hydrogen from renewable energy sources are considered. The most important results of this analysis are: (i) While for first generation biofuels, the relatively high share of fossil energy is the major problem, for second generation biofuels, the major problems are the low conversion efficiency and the corresponding high input of renewable feedstocks. Up to 2050, it is expected that these problems will be relieved, but only slightly. (ii) The energetic improvements up to 2050 will lead to substantial reduction of energetic losses in the well-to-tank as well as in the tank-to-wheel part of the energy service provision chain. (iii) By 2050, the total driving costs of all analyzed fuels and powertrains will almost even out. (iv) The major uncertainty for battery electric and fuel cell vehicles is how fast technological learning will take place especially for the battery and the fuel cells.
Amplification of the MET Receptor Drives Resistance to Anti-EGFR Therapies in Colorectal Cancer
Authors: Bardelli Alberto , Corso Simona , Bertotti Andrea , Hobor Sebastijan , Valtorta Emanuele , Siravegna Giulia , Sartore-Bianchi Andrea , Scala Elisa , Cassingena Andrea , Zecchin Davide , Apicella Maria , Migliardi Giorgia , Galimi Francesco , Lauricella Calogero , Zanon Carlo , Perera Timothy , Veronese Silvio , Corti Giorgio , Amatu Alessio , Gambacorta Marcello , Diaz Luis A. , Sausen Mark , Velculescu Victor E. , Comoglio Paolo , Trusolino Livio , Di Nicolantonio Federica , Giordano Silvia , Siena Salvatore 
Published: June 2013

Abstract
EGF receptor (EGFR)-targeted monoclonal antibodies are effective in a subset of metastatic colorectal cancers. Inevitably, all patients develop resistance, which occurs through emergence of KRAS mutations in approximately 50% of the cases. We show that amplification of the MET proto-oncogene is associated with acquired resistance in tumors that do not develop KRAS mutations during anti-EGFR therapy. Amplification of the MET locus was present in circulating tumor DNA before relapse was clinically evident. Functional studies show that MET activation confers resistance to anti-EGFR therapy both in vitro and in vivo. Notably, in patient-derived colorectal cancer xenografts, MET amplification correlated with resistance to EGFR blockade, which could be overcome by MET kinase inhibitors. These results highlight the role of MET in mediating primary and secondary resistance to anti-EGFR therapies in colorectal cancer and encourage the use of MET inhibitors in patients displaying resistance as a result of MET amplification.
Significance: Amplification of the MET proto-oncogene is responsible for de novo and acquired resistance to anti-EGFR therapy in a subset of colorectal cancers. As multiple anti-MET therapeutic strategies are available, these findings offer immediate novel opportunities to design clinical studies. Cancer Discov; 3(6); 658–73. ©2013 AACR.
This article is highlighted in the In This Issue feature, p. 591
Obesity and worsening of chronic venous disease and joint mobility
Authors: Belczak Cleusa Ema Quilici , de Godoy José Maria Pereira , Belzack Sergio Quilici , Ramos Rubiana Neves , Caffaro Roberto Augusto 
Published: June 2013

Objective The aim of this study was to investigate a possible relationship between obesity and decreased mobility of the talocrural joint and in turn chronic venous disease. Subjects and methods One hundred obese patients recruited at Hospital Santa Casa de Maringa, Parana were enrolled by order of arrival at the hospital in a randomized quantitative cross-sectional study. Inclusion criteria were patients with a body mass index above 30 kg/m2 and the exclusion criteria were infectious conditions that would interfere with the assessment. Patients were graded according to the clinical, etiological, anatomical and pathophysiological classification. Talocrural goniometry was performed to assess the degree of mobility of the legs. The Kolmogorov–Smirnov normality test, Kruskal–Wallis test, Dunn's Multiple comparison test and analysis of variance were used for statistical analysis tests with an alpha error of 5% being considered acceptable. Results The increase in body mass index is correlated to the reduction in joint mobility (Kruskal–Wallis test: p-value <0.0001) and increase in clinical, etiological, anatomical and pathophysiological classification is correlated to a decrease in joint mobility and the increase in age is associated with an increase in clinical, etiological, anatomical and pathophysiological classification (Kruskal–Wallis test: p-value <0.0001). Conclusion Obesity is associated with deterioration in joint mobility and worsening of chronic venous disease.
Sports Activity After Surgical Treatment of Intra-articular Tibial Plateau Fractures in Skiers
Authors: Loibl Markus , Bäumlein Martin , Massen Felix , Gueorguiev Boyko , Glaab Richard , Perren Thomas , Rillmann Paavo , Ryf Christian , Naal Florian D. 
Published: June 2013

Background: Tibial plateau fractures occur frequently while participating in winter sports, but there is no information on whether skiers can resume sports and recreational activities after internal fixation of these fractures. Hypothesis: Skiers can resume low-impact sports activity after internal fixation of tibial plateau fractures. Study Design: Case series; Level of evidence, 4. Methods: A total of 103 patients were surveyed by postal questionnaires to determine their sports activities at a mean of 7.8 ± 1.8 years after internal fixation of intra-articular tibial plateau fractures. The survey also included the Lysholm score, the Tegner activity scale, and a visual analog scale (VAS) for pain. Results: At the time of the survey, 88% of the patients were engaged in sports activities (rate of return to sports, 88%), and 53% continued to participate in downhill skiing. The median number of different activities declined from 5 (range, 1-17) preoperatively to 4 (range, 0-11) postoperatively ( P < .01). Sports frequency and duration per week did not change: 3 (range, 1-7) preoperatively versus 3 (range, 0-7) postoperatively ( P = .275) and 4 hours (range, 1-16 hours) preoperatively versus 3.5 hours (range, 0-15 hours) postoperatively ( P = .217), respectively. Median values of all outcome scores declined: Lysholm score, 100 (range, 85-100) preoperatively versus 94.5 (range, 37-100) postoperatively ( P < .01); VAS, 0 (range, 0-7) preoperatively versus 1 (range, 0-8) postoperatively ( P < .01). Median Tegner activity scale scores declined in all age groups except for patients aged 51 to 60 years. The ability to participate in sports at the time of follow-up compared with the ability before the accident was rated as “similar” by 57 patients (62.0%) and as “worse” by 35 patients (38.0%). The more severe fracture types, B3 and C3 according to the AO classification system, were associated with poorer outcomes related to return to sports and functional scores. Conclusion: A large percentage of skiers with surgically treated intra-articular tibial plateau fractures cannot continue to participate in downhill skiing; however, the majority could resume an active lifestyle for several years after the trauma. Fracture type seems to be an important factor influencing physical activity and general functional outcome.
The Effects of Meniscus Injury on the Development of Knee Osteoarthritis
Authors: Badlani Jayshiv T. , Borrero Camilo , Golla Saraswati , Harner Christopher D. , Irrgang James J. 
Published: June 2013

Background: Osteoarthritis (OA) is a leading cause of pain and disability, and the knee is one of the most commonly affected joints. Many have speculated that injury to the meniscus may play a key role in the development of knee OA; however, the exact relationship of meniscus injury and knee OA is unknown. Purpose: To examine the rate of meniscus injuries in patients with and without incident radiographic knee OA and to explore how the type, size, and location of the meniscus injury affects the development of OA. Study Design: Case-control study; Level of evidence, 3. Methods: A secondary case-control analysis was conducted of the Knee Osteoarthritis Initiative (OAI) database, which is a prospective, longitudinal, multicenter cohort study of knee health in patients at risk for knee OA. Eligible patients included those without radiographic OA at baseline in 1 knee. Thirty-two patients who developed radiographic OA in a previously unaffected knee after 2 years of follow-up were matched to 64 age-, sex-, and body mass index–matched patients who did not develop OA. The study analyzed the relationship between the development of OA and the presence of a meniscus tear or degenerative signal, meniscus extrusion, tear type, and size of injury on the baseline magnetic resonance imaging scans. Conditional logistic regression was used to identify significant predictors of OA. Results: The rate of medial meniscus lesions (tear or degeneration) was not significantly higher in those who developed incident OA (85%) compared with the control patients (68%; P = .07). However, medial meniscus extrusion (odds ratio [OR], 3.03; 95% confidence interval [CI], 1.4-6.5), complex tears (OR, 5.0; 95% CI, 1.0-25), and tears with large radial involvement (OR, 5.92; 95% CI, 1.7-7.5) were more common at baseline in cases compared with controls. Conclusion: Knees with meniscus tears with greater radial involvement and extrusion are at greater risk for later development of radiographic OA.
A case of paroxysmal nocturnal hemoglobinuria caused by a germline mutation and a somatic mutation in PIGT
Authors: Krawitz Peter M. , Höchsmann Britta , Murakami Yoshiko , Teubner Britta , Krüger Ulrike , Klopocki Eva , Neitzel Heidemarie , Hoellein Alexander , Schneider Christina , Parkhomchuk Dmitri , Hecht Jochen , Robinson Peter N. , Mundlos Stefan , Kinoshita Taroh , Schrezenmeier Hubert 
Published: June 2013

Key Points
A carrier of a deleterious splice site mutation in PIGT acquired a second hit in PIGT and developed PNH.
PMTED: a plant microRNA target expression database
Authors: Sun Xiuli , Dong Boquan , Yin Lingjie , Zhang Rongzhi , Du Wei , Liu Dongfeng , Shi Nan , Li Aili , Liang Yanchun , Mao Long 
Published: June 2013

Abstract

Background
MicroRNAs (miRNAs) are identified in nearly all plants where they play important roles in development and stress responses by target mRNA cleavage or translation repression. MiRNAs exert their functions by sequence complementation with target genes and hence their targets can be predicted using bioinformatics algorithms. In the past two decades, microarray technology has been employed to study genes involved in important biological processes such as biotic response, abiotic response, and specific tissues and developmental stages, many of which are miRNA targets. Despite their value in assisting research work for plant biologists, miRNA target genes are difficult to access without pre-processing and assistance of necessary analytical and visualization tools because they are embedded in a large body of microarray data that are scattered around in public databases.

Description
Plant MiRNA Target Expression Database (PMTED) is designed to retrieve and analyze expression profiles of miRNA targets represented in the plethora of existing microarray data that are manually curated. It provides a Basic Information query function for miRNAs and their target sequences, gene ontology, and differential expression profiles. It also provides searching and browsing functions for a global Meta-network among species, bioprocesses, conditions, and miRNAs, meta-terms curated from well annotated microarray experiments. Networks are displayed through a Cytoscape Web-based graphical interface. In addition to conserved miRNAs, PMTED provides a target prediction portal for user-defined novel miRNAs and corresponding target expression profile retrieval. Hypotheses that are suggested by miRNA-target networks should provide starting points for further experimental validation.

Conclusions
PMTED exploits value-added microarray data to study the contextual significance of miRNA target genes and should assist functional investigation for both miRNAs and their targets. PMTED will be updated over time and is freely available for non-commercial use at http://pmted.agrinome.org.
Cancer testis antigens and NY-BR-1 expression in primary breast cancer: prognostic and therapeutic implications
Authors: Balafoutas Dimitrios , zur Hausen Axel , Mayer Sebastian , Hirschfeld Marc , Jaeger Markus , Denschlag Dominik , Gitsch Gerald , Jungbluth Achim , Stickeler Elmar 
Published: June 2013

AbstractBackgroundCancer–testis antigens (CTA) comprise a family of proteins, which are physiologically expressed in adult human tissues solely in testicular germ cells and occasionally placenta. However, CTA expression has been reported in various malignancies. CTAs have been identified by their ability to elicit autologous cellular and or serological immune responses, and are considered potential targets for cancer immunotherapy. The breast differentiation antigen NY-BR-1, expressed specifically in normal and malignant breast tissue, has also immunogenic properties. Here we evaluated the expression patterns of CTAs and NY-BR-1 in breast cancer in correlation to clinico-pathological parameters in order to determine their possible impact as prognostic factors.MethodsThe reactivity pattern of various mAbs (6C1, MA454, M3H67, 57B, E978, GAGE #26 and NY-BR-1 #5) were assessed by immunohistochemistry in a tissue micro array series of 210 randomly selected primary invasive breast cancers in order to study the diversity of different CTAs (e.g. MAGE-A, NY-ESO-1, GAGE) and NY-BR-1. These expression data were correlated to clinico-pathological parameters and outcome data including disease-free and overall survival.ResultsExpression of at least one CTA was detectable in the cytoplasm of tumor cells in 37.2% of the cases. NY-BR-1 expression was found in 46.6% of tumors, respectively. Overall, CTA expression seemed to be linked to adverse prognosis and M3H67 immunoreactivity specifically was significantly correlated to shorter overall and disease-free survival (p=0.000 and 0.024, respectively).ConclusionsOur findings suggest that M3H67 immunoreactivity could serve as potential prognostic marker in primary breast cancer patients. The exclusive expression of CTAs in tumor tissues as well as the frequent expression of NY-BR-1 could define new targets for specific breast cancer therapies.
Marine algal natural products with anti-oxidative, anti-inflammatory, and anti-cancer properties
Authors: Lee Jin-Ching , Hou Ming-Feng , Huang Hurng-Wern , Chang Fang-Rong , Yeh Chi-Chen , Tang Jen-Yang , Chang Hsueh-Wei 
Published: June 2013

Abstract
For their various bioactivities, biomaterials derived from marine algae are important ingredients in many products, such as cosmetics and drugs for treating cancer and other diseases. This mini-review comprehensively compares the bioactivities and biological functions of biomaterials from red, green, brown, and blue-green algae. The anti-oxidative effects and bioactivities of several different crude extracts of algae have been evaluated both in vitro a nd in vivo. Natural products derived from marine algae protect cells by modulating the effects of oxidative stress. Because oxidative stress plays important roles in inflammatory reactions and in carcinogenesis, marine algal natural products have potential for use in anti-cancer and anti-inflammatory drugs.
Natural Plasmodium infection in monkeys in the state of Rondônia (Brazilian Western Amazon)
Authors: Araújo Maisa S , Messias Mariluce R , Figueiró Marivaldo R , Gil Luiz Herman S , Probst Christian M , Vidal Newton M , Katsuragawa Tony H , Krieger Marco A , Pereira da Silva Luiz H , Ozaki Luiz S 
Published: June 2013

Abstract

Background
Simian malaria is still an open question concerning the species of Plasmodium parasites and species of New World monkeys susceptible to the parasites. In addition, the lingering question as to whether these animals are reservoirs for human malaria might become important especially in a scenario of eradication of the disease. To aid in the answers to these questions, monkeys were surveyed for malaria parasite natural infection in the Amazonian state of Rondônia, Brazil, a state with intense environmental alterations due to human activities, which facilitated sampling of the animals.

Methods
Parasites were detected and identified in DNA from blood of monkeys, by PCR with primers for the 18S rRNA, CSP and MSP1 genes and sequencing of the amplified fragments. Multiplex PCR primers for the 18S rRNA genes were designed for the parasite species Plasmodium falciparum and Plasmodium vivax, Plasmodium malariae/Plasmodium brasilianum and Plasmodium simium.

Results
An overall infection rate of 10.9% was observed or 20 out 184 monkey specimens surveyed, mostly by P. brasilianum. However, four specimens of monkeys were found infected with P. falciparum, two of them doubly infected with P. brasilianum and P. falciparum. In addition, a species of monkey of the family Aotidae, Aotus nigriceps, is firstly reported here naturally infected with P. brasilianum. None of the monkeys surveyed was found infected with P. simium/P. vivax.

Conclusion
The rate of natural Plasmodium infection in monkeys in the Brazilian state of Rondônia is in line with previous surveys of simian malaria in the Amazon region. The fact that a monkey species was found that had not previously been described to harbour malaria parasites indicates that the list of monkey species susceptible to Plasmodium infection is yet to be completed. Furthermore, finding monkeys in the region infected with P. falciparum clearly indicates parasite transfer from humans to the animals. Whether this parasite can be transferred back to humans and how persistent the parasite is in monkeys in the wild so to be efficient reservoirs of the disease, is yet to be evaluated. Finding different species of monkeys infected with this parasite species suggests indeed that these animals can act as reservoirs of human malaria.
Electrochemical synthesis of ZnO branched submicrorods on carbon fibers and their feasibility for environmental applications
Authors: Ko Yeong Hwan , Ramana Dandu Kamakshigari Venkata , Yu Jae Su 
Published: June 2013

AbstractWe investigated the structural and optical properties of the hierarchically integrated zinc oxide (ZnO) branched submicrorods on carbon fibers (ZOCF) by scanning/transmission electron microscopy, X-ray diffraction, and photoluminescence (PL) measurements. The ZnO submicrorods were facilely synthesized by an electrochemical deposition method on polyacrylonitrile-based carbon fiber sheets used as a substrate. After coating the ZnO seed layer on the surface of the carbon fibers, ZnO submicrorods were densely grown on the nuclei sites of the seed layer. The prepared ZOCF samples exhibited high crystallinity and good PL properties. A feasibility for environmental application in Pb(II) removal from aqueous solutions was also studied. The ZOCF adsorbent exhibited an excellent maximum adsorption capacity of 245.07 mg g−1, which could be practically used in Pb(II) removal from water. These fabricated ZOCFs are potentially useful for multifunctional and environmental devices.