bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural pathways mediating control of reproductive behavior in male Japanese quail
Authors: Wild J. Martin , Balthazart Jacques 
Published: December 2012

AbstractThe sexually dimorphic medial preoptic nucleus (POM) in Japanese quail has for many years been the focus of intensive investigations into its role in reproductive behavior. The present study delineates a sequence of descending pathways that finally reach sacral levels of the spinal cord housing motor neurons innervating cloacal muscles involved in reproductive behavior. We first retrogradely labeled the motor neurons innervating the large cloacal sphincter muscle (mSC) that forms part of the foam gland complex (Seiwert and Adkins‐Regan [1998] Brain Behav Evol 52:61–80) and then putative premotor nuclei in the brainstem, one of which was nucleus retroambigualis (RAm) in the caudal medulla. Anterograde tracing from RAm defined a bulbospinal pathway, terminations of which overlapped the distribution of mSC motor neurons and their extensive dorsally directed dendrites. Descending input to RAm arose from an extensive dorsomedial nucleus of the intercollicular complex (DM‐ICo), electrical stimulation of which drove vocalizations. POM neurons were retrogradely labeled by injections of tracer into DM‐ICo, but POM projections largely surrounded DM, rather than penetrated it. Thus, although a POM projection to ICo was shown, a POM projection to DM must be inferred. Nevertheless, the sequence of projections in the male quail from POM to cloacal motor neurons strongly resembles that in rats, cats, and monkeys for the control of reproductive behavior, as largely defined by Holstege et al. ([1997], Neuroscience 80:587–598). J. Comp. Neurol. 521:2067–2087, 2013. © 2012 Wiley Periodicals, Inc.
Expression patterns of Pax6 and Pax7 in the adult brain of a urodele amphibian, Pleurodeles waltl
Authors: Joven Alberto , Morona Ruth , González Agustín , Moreno Nerea 
Published: December 2012

AbstractExpression patterns of Pax6, Pax7, and, to a lesser extent, Pax3 genes were analyzed by a combination of immunohistochemical techniques in the central nervous system of adult specimens of the urodele amphibian Pleurodeles waltl. Only Pax6 was found in the telencephalon, specifically the olfactory bulbs, striatum, septum, and lateral and central parts of the amygdala. In the diencephalon, Pax6 and Pax7 were distinct in the alar and basal parts, respectively, of prosomere 3. The distribution of Pax6, Pax7, and Pax3 cells correlated with the three pretectal domains. Pax7 specifically labeled cells in the dorsal mesencephalon, mainly in the optic tectum, and Pax6 cells were the only cells found in the tegmentum. Large populations of Pax7 cells occupied the rostral rhombencephalon, along with lower numbers of Pax6 and Pax3 cells. Pax6 was found in most granule cells of the cerebellum. Pax6 cells also formed a column of scattered neurons in the reticular formation and were found in the octavolateral area. The rhombencephalic ventricular zone of the alar plate expressed Pax7. Dorsal Pax7 cells and ventral Pax6 cells were found along the spinal cord. Our results show that the expression of Pax6 and Pax7 is widely maintained in the brains of adult urodeles, in contrast to the situation in other tetrapods. This discrepancy could be due to the generally pedomorphic features of urodele brains. Although the precise role of these transcription factors in adult brains remains to be determined, our findings support the idea that they may also function in adult urodeles. J. Comp. Neurol. 521:2088–2124, 2013. © 2012 Wiley Periodicals, Inc.
Composing and scheduling service‐oriented applications in time‐triggered distributed real‐time Java environments
Authors: Estévez‐Ayres Iria , Basanta‐Val Pablo , García‐Valls Marisol 
Published: December 2012

SUMMARYDuring the last decade, the number of distributed application domains with temporal requirements has significantly augmented, arising the necessity of exploring new concepts and paradigms that allow, on the one hand, the development of dynamic and flexible distributed applications and, on the other hand, the reusability of code. Service‐oriented paradigms have been successfully applied to distributed environments, increasing their flexibility and allowing the reusability of their components. Besides, distributed real‐time Java technologies have shown to be a good candidate to deploy real‐time distributed applications. This paper presents a model for service‐oriented applications on a time‐triggered distributed real‐time Java environment, focusing on the definition of the temporal model of an application and its schedulability, applying and evaluating this model in real‐time service‐oriented composition algorithms. Copyright © 2012 John Wiley & Sons, Ltd.
Implementing Evidence‐Based Practice for Patients with Chronic Fatigue Syndrome
Authors: Wiborg Jan F. , Wensing Michel , Tummers Marcia , Knoop Hans , Bleijenberg Gijs 
Published: December 2012

The aim of our study was to explore whether community‐based mental health care centres (MHCs) are able to implement and sustain cognitive behaviour therapy (CBT) for chronic fatigue syndrome (CFS) with the help of an implementation manual. We monitored the implementation process and treatment outcome data of three Dutch MHCs that implemented or sustained CBT for CFS, one in the context of a stepped care programme. We compared these data with findings of other treatment studies conducted in the context of CBT for CFS. All three MHCs included at least 40 patients with dropout rates between 15% and 35% from intention‐to‐treat to second assessment. Effect sizes ranged between 0.88 and 1.76 for changes in fatigue severity and 0.43 and 1.23 for changes in physical functioning. With one exception, these outcomes were within the range of our benchmark. Contrary to original expectations, we provided additional implementation support to the two MHCs new with CBT for CFS. We concluded that our implementation manual does not seem to substitute external support for team leaders and associated professions during initial implementation of CBT for CFS but may have the potential to make this assistance more efficient. Particular attention should be paid to challenges of implementing stepped care for CFS. Copyright © 2012 John Wiley & Sons, Ltd.Key Practitioners Message:

Implementation of CBT for CFS in community‐based MHCs was monitored.
External support was provided in addition to an implementation manual during initial implementation of CBT for CFS.
Participating MHCs were generally capable of successfully implementing and delivering CBT for CFS.
Implementation of low‐intensity interventions for CFS might better be postponed until therapists have sufficient experience with conventional CBT for CFS.
Slit/Robo‐mediated chemorepulsion of vagal sensory axons in the fetal gut
Authors: Goldberg David , Borojevic Rajka , Anderson Monique , Chen Jason J. , Gershon Michael D. , Ratcliffe Elyanne M. 
Published: December 2012

AbstractCOVER PHOTOGRAPH: Fetal mouse gut at E14. Nuclei are illuminated via bisbenzimide fluorescence (blue). Primary antibodies have been omitted; non‐specific immunofluorence can be seen from the application of secondary donkey anti‐goat antibodies labeled with Alexa 488 (green) and goat anti‐chicken antibodies labeled with Alexa 594 (red). From Goldberg et al., Developmental Dynamics 242:9–15.
Transposon mediated BAC transgenesis via pronuclear injection of mouse zygotes
Authors: Rostovskaya Maria , Naumann Ronald , Fu Jun , Obst Mandy , Mueller Doris , Stewart A. Francis , Anastassiadis Konstantinos 
Published: December 2012

AbstractPronuclear microinjection of bacterial artificial chromosomes (BACs) is the preferred way to generate transgenic mice because the transgene accurately recapitulates expression of the endogenous gene. However, the method is demanding and the integrity and copy number of the BAC transgene is difficult to control. Here, we describe a simpler pronuclear injection method that relies on transposition to introduce full‐length BACs into the mouse genome. The bacterial backbone of a hPAX6‐GFP reporter BAC was retrofitted with PiggyBac transposon inverted terminal repeats and co‐injected with PiggyBac transposase mRNA. Both the frequency of transgenic founders as well as intact, full‐length, single copy integrations were increased. Transposition was determined by a rapid PCR screen for a transpositional signature and confirmation by splinkerette sequencing to show that theBACs were integrated as a single copy either in one or two different genomic sites. BAC transposons displayed improved functional accuracy over random integrants as evaluated by expression of the hPAX6‐GFP reporter in embryonic neural tube and absence of ectopic expression. This method involves less work to achieve increased frequencies of both transgenesis and single copy, full‐length integrations. These advantages are not only relevant to rodents but also for transgenesis in all systems. genesis 51:135–141, 2013. © 2012 Wiley Periodicals, Inc.
Crucial Influence of the Intramolecular Hydrogen Bond on the Coordination Mode of RC(S)NHP(S)(OiPr)2 in Homoleptic Complexes with NiII
Authors: Babashkina Maria G. , Safin Damir A. , Srebro Monika , Kubisiak Piotr , Mitoraj Mariusz P. , Bolte Michael , Garcia Yann 
Published: December 2012

AbstractReaction of the deprotonated N‐thiophosphorylated thioureas RC(S)NHP(S)(OiPr)2 [R = EtNH (HLI), iPrNH (HLII), Et2N (HLIII), 2,5‐Me2C6H3NH (HLIV), 4‐Me2NC6H4NH (HLV)] with NiII leads to complexes of the formula [NiLI–V2]. The molecular structures of the complexes in the solid were elucidated by single‐crystal X‐ray diffraction analysis. In the complexes, the metal atom is found to be in a square‐planar trans‐N2S2 ([NiLII,IV2]) environment formed by the C=S sulfur atoms and the P–N nitrogen atoms, or in a square‐planar trans‐S2S′2 ([NiLI,III2]) environment formed by the C=S and P=S sulfur atoms of two deprotonated ligands. Reaction of deprotonated N‐thiophosphorylated thiourea HLV with NiCl2 leads to violet [Ni(L‐1,3‐N,S)2] or dark violet [Ni(L‐1,5‐S,S′)2]·(CH3)2C=O crystals that were isolated by recrystallization from a mixture of CH2Cl2 or acetone, respectively, and n‐hexane. DFT calculations confirmed that the [Ni(LI,II,IV,V‐N,S)2] conformers are more stable (by 5–7 kcal/mol) than [Ni(LI,II,IV,V‐S,S′)2], whereas [Ni(LIII‐N,S)2] is less stable (by 7–9 kcal/mol) than [Ni(LIII‐S,S′)2]. The main reason for higher stability of the 1,3‐N,S versus 1,5‐S,S′ isomers is the formation of intramolecular N–H···S=P hydrogen bonds. The same hydrogen bonds are impossible in complex [NiLIII2]. In solution, complex [NiLIII2] has revealed an exclusively 1,5‐S,S′ coordination, whereas compounds [NiLI,II,IV,V2] reveal at least two isomers in the 1H and 31P{1H} NMR spectra. The major species is assigned to the 1,3‐N,S‐coordinated isomer, and the minor signals are assigned to the 1,5‐S,S′ isomer, which was confirmed by UV/Vis spectroscopic results. The electrochemical measurements reveal reversible one‐electron reduction and irreversible oxidations both assigned to ligand‐centred processes. Ligand‐based oxidation processes agree well with TD‐DFT results.
Alkaloids from the Sponge‐Associated Fungus Aspergillus sp.
Authors: Zhou Yaming , Debbab Abdessamad , Mándi Attila , Wray Victor , Schulz Barbara , Müller Werner E. G. , Kassack Matthias , Lin WenHan , Kurtán Tibor , Proksch Peter , Aly Amal H. 
Published: December 2012

AbstractSeven new alkaloids including tryptoquivaline K (1) and fumiquinazolines K–P (2–7), bearing a rare 1‐aminocyclopropane‐1‐carboxylic acid residue, together with six known compounds (8–13), were isolated from the fungus Aspergillus sp. obtained from the Mediterranean sponge Tethya aurantium. The structures of the new compounds were determined by extensive analysis by 1D and 2D NMR spectroscopy and mass spectrometry. The absolute configurations of tryptoquivaline K (1) and fumiquinazolines K and L (2, 3) were determined by TDDFT ECD calculations of their solution conformers, and the configurational assignment of the related fumiquinazolines M–P (4–7) was achieved by comparison of their ECD spectra with those of compounds 2 and 3. All compounds were evaluated for their cytotoxic activity by the MTT method. Compound 8 exhibited pronounced cytotoxicity against the mouse lymphoma cell line (L5178Y) with an IC50 value of 3.6 μM, but only moderate activity was observed against human ovarian cancer (A2780) and human Philadelphia chromosome‐positive chronic myelogenous leukemia (K562) cell lines with IC50 values of 18.5 and 15.0 μM, respectively.
Nanodiamond Decorated with Silver Nanoparticles as a Sensitive Film Modifier in a Jeweled Electrochemical Sensor: Application to Voltammetric Determination of Thioridazine
Authors: Shahrokhian Saeed , Hosseini Nassab Niloufar 
Published: December 2012

AbstractNanodiamondgraphite (NDG) decorated with Ag nanoparticles (AgNPs‐NDG) was prepared and used to construct a novel sensitive sensor for the voltammetric determination of thioridazine (TR). The results indicate a remarkable increase in the oxidation peak currents together with a negative shift in the oxidation peak potentials, in comparison to the bare pyrolytic graphite electrode. Remarkable enhancement in microscopic area of the electrode along with strong adsorption of TR on the surface of the modified electrode resulted in a considerable increase in the peak current of TR. The surface morphology and the nature of the composite film deposited on PGE were characterized by scanning electron microscopy, atomic force microscopy, cyclic voltammetry and electrochemical impedance spectroscopy. Experimental variables, such as the deposited amount of the modifier suspension, pH of the supporting electrolyte, the accumulation potential and time are optimized by monitoring the CV responses of TR. Under the optimal conditions, the modified electrode showed a wide linear response to the concentration of TR in the range of 0.08–100 µM with a detection limit of 0.01 µM. The prepared modified electrode showed several advantages: simple preparation method, high stability and uniformity in the composite film, high sensitivity, long‐term stability and remarkable voltammetric reproducibility in response to TR. The modified electrode can be successfully applied for accurate determination of trace amounts of TR in pharmaceutical and clinical preparations.
Carbon Nanotube, Carbon Black and Copper Nanoparticle Modified Screen Printed Electrodes for Amino Acid Determination
Authors: Carvalho Ricardo C. , Mandil Adil , Prathish Krishna P. , Amine Aziz , Brett Christopher M. A. 
Published: December 2012

AbstractScreen‐printed electrodes have been used for the determination of amino acids, focussing particularly on tryptophan, following different surface modifications by carbon nanotubes (CNT), carbon black (CB) and copper nanoparticles. The modified electrodes were characterised by cyclic voltammetry, electrochemical impedance spectroscopy and scanning electron microscopy. Detection by square wave voltammetry was improved by further surface modification with copper nanoparticles, exhibiting efficient electrocatalytic activity towards amino acid oxidation with high sensitivity, stability and long shelf‐life. The applicability of a low‐cost, robust carbon black substrate using the synergetic electrocatalytic effect provided by copper nanoparticles for amino acid sensing is demonstrated.
Enhanced Anodic Electrochemiluminescence from Co2+‐Doped CdSe Nanocrystals for Alkaline Phosphatase Assay
Authors: Wang Jing , Zhang Yan‐Yan , Zhao Wei‐Wei , Xu Jing‐Juan , Chen Hong‐Yuan 
Published: December 2012

AbstractCo2+ doped CdSe nanocrystals (CdSe:Co NCs) have been synthesized via a co‐precipitation method in homogeneous solutions. The anodic electrochemiluminescence (ECL) of CdSe:Co NCs with tripropylamine (TPrA) as co‐reactant was studied in aqueous solution for the first time. Co2+ doped IIVI NCs systems exhibit strong coupling due to Co d‐orbital mixing with the valence band and conduction band of the IIVI host, which causes a ca. 3‐fold enhancement in ECL intensity compared with the undoped CdSe NCs. Benefiting from the strong anodic ECL emission of the doped NCs, we developed an alkaline phosphatase (ALP)‐responsive ECL system to detect the enzymatic processes successfully. An inhibition type anodic ECL strategy has been used to sensitively detect ALP ranging from 0.5 nM to 10 nM, with a detection limit of 0.1 nM.
Chemically Modified Graphenes as Detectors in Lab‐on‐Chip Device
Authors: Chua Chun Kiang , Pumera Martin 
Published: December 2012

AbstractGraphene materials hold immense potentials for electrochemical detectors in lab‐on‐chip devices. The electronic and electrochemical properties of graphene based materials are significantly affected by the fabrication routes and by the structural features, such as density of defects and amount of oxygen containing groups. Therefore it is paramount to evaluate various graphene‐based materials prior to their integration onto the lab‐on‐chip devices. The performance of various reduced graphenes (so called chemically modified graphene materials), namely, thermally, chemically and electrochemically reduced graphenes as well as graphene‐oxide and graphite‐oxide as detectors in a microfluidics system was examined and linked to the materials properties of the various graphenes. This work shows that not all graphene materials are beneficial for the detection at lab‐on‐chip devices. In addition, the findings show that materials exhibiting excellent properties in batch measurements demonstrate poor performance in flow‐setup. These findings could provide valuable insights into the future applicability of graphene materials towards practical applications.
Advisory committees in contract and grant‐funded evaluation projects
Authors: Mattessich Paul W. 
Published: December 2012

AbstractThis chapter describes the use of evaluation advisory committees in contract and grant‐funded evaluation projects. It defines what constitutes an evaluation advisory committee. It identifies five key functions of evaluation advisory committees: stakeholder engagement, maximizing external credibility, political conciliation, promotion of methodological integrity, and promotion of use. Typical features are explained: size, composition, meeting content, expectations for authority, and decision making. Whether to have an advisory committee is discussed. A range of examples—from fields of human services, public health, education, and others—illustrates the uses, functions, features, and compositional options of committees. ©Wiley Periodicals, Inc., and the American Evaluation Association.
Advice giving in contested space
Authors: VeLure Roholt Ross 
Published: December 2012

AbstractShortly after the ratification of the peace accords, the national museum of Northern Ireland created an exhibition on the legacy of The Troubles, a term used to refer to the previous 30 years of violent civil strife. This exhibition aimed to address the conflicted understanding of this time period through the inclusion of multiple stories, artifacts representing both communities, and ongoing input from museum visitors. As anticipated, the exhibition was contested even prior to its opening. An evaluator was contracted to assess visitor satisfaction and learning outcomes, and provided with an evaluation advisory group (EAG) that consisted of two high‐ranking museum staff and a high‐ranking museum professional who had worked on regional and national issues for museums. This case study will describe how this EAG provided the necessary political coverage to allow the evaluation to be designed, carried out, and used. ©Wiley Periodicals, Inc., and the American Evaluation Association.
Congratulations on the new initiative! Is it time for a new committee?
Authors: Block Betsy Baum 
Published: December 2012

AbstractUnited Way of the Bay Area (UWBA) has started to implement its 10‐year initiative: Cut Poverty in Half in the Bay Area by 2020, in partnership with hundreds of organizations. The goal is audacious, and the effort required immense. As a founding steering council begins to move forward with all the complexities of this initiative, among the many challenges is to answer how our progress and impact can feasibly be measured. The concept of an evaluation advisory committee (EAC) holds tremendous appeal as a way to leverage the best experts to provide critical input and help us define credible evidence for success. In developing the EAC, we found no ready‐made guide and began constructing our own approach. This chapter discusses considerations for the framework of an EAC, and shares some key decisions about its purpose and structure. It shares our initial goals, the proposed composition of the EAC, and how our understanding of engaging an EAC has evolved. © Wiley Periodicals, Inc., and the American Evaluation Association.
Molecular and environmental factors determining grain quality in rice
Authors: Chen Yi , Wang Mei , Ouwerkerk Pieter B. F. 
Published: December 2012

AbstractRice among other cereals is key to food security for at least half the world population. Since the 1960s, productivity of rice has largely been improved during the Green Revolution, which included development of new cultivars, irrigation infrastructure, new management techniques, and synthetic fertilizers and pesticides. Nowadays, scientists and breeders are more and more focused on improving the quality of rice for different purposes and markets. For instance, people in the Far East prefer sticky and soft rice, while in India, a non‐sticky type is preferred. Consumers from developed countries ask mainly for grain with good cooking quality and eating characteristics, but in many developing regions, nutritional value is crucial as rice is the most consumed staple food. Grain quality is a general concept which covers many characteristics ranging from physical to biochemical and physiological properties. Starch and protein are the two main components of rice endosperm and therefore are key to quality. The knowledge of how starch and protein are synthesized, sorted, and stored in starch granules and protein bodies (PB) is important for rice breeding. Besides that, grain quality has been shown to be affected significantly by growing and environmental conditions, such as water availability, temperature, fertilizer application, drought, and salinity stresses. However, the signal transduction pathways controlling grain quality still remain largely unclear. In the following sections, we first briefly review the four main aspects of grain quality, followed by a discussion of the molecular and genetic basis of starch and seed‐storage protein biosynthesis and the effects of environmental factors. Obviously, rice grain is also an important source of mineral micronutrients, as well as important vitamins. Storage of these also plays crucial roles in grain quality and nutritional value, but we will only discuss these aspects briefly in this review.