bluuebunny/crossref_metadata_2025_split huggingface dataset:
1H-pyrrole-2,5-dione-based small molecule-induced generation of mesenchymal stem cell-derived functional endothelial cells that facilitate rapid endothelialization after vascular injury
Authors: Song Byeong-Wook , Kim Il-Kwon , Lee Seahyoung , Choi Eunhyun , Ham Onju , Lee Se-Yeon , Lee Chang Yeon , Park Jun-Hee , Lee Jiyun , Seo Hyang-Hee , Chang Woochul , Yoon Cheesoon , Hwang Ki-Chul 
Published: September 2015

Abstract
Introduction
Despite the success of interventional processes such as drug-eluting stents, complete prevention of restenosis is still hindered by impaired or delayed endothelialization or both. Here, we report that 1H-pyrrole-2,5-dione-based small molecule-generated mesenchymal stem cell-derived functional endothelial cells (MDFECs) facilitated rapid transmural coverage of injured blood vessels.

Methods
Small molecules that induced CD31 expression were screened by principal component analysis (PCA). Rat mesenchymal stem cells (MSCs) were treated with selected small molecules for up to 16 days, and the expression levels of CD90 and CD31 were examined by immunocytochemistry. In vitro functional assays of MDFECs, including tube formation assays and nitric oxide production assays, were performed. After MDFECs (intravenous, 3×106 cells per animal) were injected into balloon-injured rats, neointima formation was monitored for up to 21 days. The endothelial coverage of denuded blood vessels was evaluated by Evans Blue staining. The functionality of repaired blood vessels was evaluated by measuring vasorelaxation and hemodynamic changes. Additionally, derivatives of the selected small molecules were examined for their ability to induce endothelial markers.

Results
PCA indicated that 3-(2,4-dichlorophenyl)-4-(1-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione effectively induced MDFECs. MDFECs inhibited the neointima formation of denuded blood vessels by facilitating more rapid endothelialization. Further examination indicated that derivatives with a 1H-pyrrole-2,5-dione moiety are important for initiating the endothelial cell differentiation of MSCs.

Conclusions
Small molecules with 1H-pyrrole-2,5-dione as a core structure have great potential to improve the efficacy of MSC-based cell therapy for vascular diseases, such as atherosclerosis and restenosis.
The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries
Authors: Zhou Yiqin , Zhang Jianying , Wu Haishan , Hogan MaCalus V. , Wang James H-C. 
Published: September 2015

Abstract
Introduction
Platelet-rich plasma (PRP) is widely used to treat tendon injuries in clinics. These PRP preparations often contain white blood cells or leukocytes, and the precise cellular effects of leukocyte-rich PRP (L-PRP) on tendons are not well defined. Therefore, in this study, we determined the effects of L-PRP on tendon stem/progenitor cells (TSCs), which play a key role in tendon homeostasis and repair.

Methods
TSCs isolated from the patellar tendons of rabbits were treated with L-PRP or P-PRP (pure PRP without leukocytes) in vitro, followed by measuring cell proliferation, stem cell marker expression, inflammatory gene expression, and anabolic and catabolic protein expression by using immunostaining, quantitative real-time polymerase chain reaction, Western blot, and enzyme-linked immunosorbent assay, respectively.

Results
Cell proliferation was induced by both L-PRP and P-PRP in a dose-dependent manner with maximum proliferation at a 10 % PRP dose. Both PRP treatments also induced differentiation of TSCs into active tenocytes. Nevertheless, the two types of PRP largely differed in several effects exerted on TSCs. L-PRP induced predominantly catabolic and inflammatory changes in differentiated tenocytes; its treatment increased the expression of catabolic marker genes, matrix metalloproteinase-1 (MMP-1), MMP-13, interleukin-1beta (IL-1β), IL-6 and tumor necrosis factor-alpha (TNF-α), and their respective protein expression and prostaglandin E2 (PGE2) production. In contrast, P-PRP mainly induced anabolic changes; that is, P-PRP increased the gene expression of anabolic genes, alpha-smooth muscle actin (α-SMA), collagen types I and III.

Conclusions
These findings indicate that, while both L-PRP and P-PRP appear to be “safe” in inducing TSC differentiation into active tenocytes, L-PRP may be detrimental to the healing of injured tendons because it induces catabolic and inflammatory effects on tendon cells and may prolong the effects in healing tendons. On the other hand, when P-PRP is used to treat acutely injured tendons, it may result in the formation of excessive scar tissue due to the strong potential of P-PRP to induce inordinate cellular anabolic effects.
Splicing Factor Prp8 Interacts With NESAR and Regulates Androgen Receptor in Prostate Cancer Cells
Authors: Wang Dan , Nguyen Minh M. , Masoodi Khalid Z. , Singh Prabhpreet , Jing Yifeng , O'Malley Katherine , Dar Javid A. , Dhir Rajiv , Wang Zhou 
Published: September 2015

AbstractAndrogen receptor (AR) plays a pivotal role in the development of primary as well as advanced castration-resistant prostate cancer. Previous work in our lab identified a novel nuclear export signal (NES) (NESAR) in AR ligand-binding domain essential for AR nucleocytoplasmic trafficking. By characterizing the localization of green fluorescence protein (GFP)-tagged NESAR, we designed and executed a yeast mutagenesis screen and isolated 7 yeast mutants that failed to display the NESAR export function. One of those mutants was identified as the splicing factor pre-mRNA processing factor 8 (Prp8). We further showed that Prp8 could regulate NESAR function using short hairpin RNA knockdown of Prp8 coupled with a rapamycin export assay in mammalian cells and knockdown of Prp8 could induce nuclear accumulation of GFP-tagged AR in PC3 cells. Prp8 expression was decreased in castration-resistant LuCaP35 xenograft tumors as compared with androgen-sensitive xenografts. Laser capture microdissection and quantitative PCR showed Prp8 mRNA levels were decreased in human prostate cancer specimens with high Gleason scores. In prostate cancer cells, coimmunoprecipitation and deletion mutagenesis revealed a physical interaction between Prp8 and AR mainly mediated by NESAR. Luciferase assay with prostate specific antigen promoter-driven reporter demonstrated that Prp8 regulated AR transcription activity in prostate cancer cells. Interestingly, Prp8 knockdown also increased polyubiquitination of endogenous AR. This may be 1 possible mechanism by which it modulates AR activity. These results show that Prp8 is a novel AR cofactor that interacts with NESAR and regulates AR function in prostate cancer cells.
A zebrafish model of inflammatory lymphangiogenesis
Authors: Okuda Kazuhide S. , Misa June Pauline , Oehlers Stefan H. , Hall Christopher J. , Ellett Felix , Alasmari Sultan , Lieschke Graham J. , Crosier Kathryn E. , Crosier Philip S. , Astin Jonathan W. 
Published: September 2015

ABSTRACT
Inflammatory bowel disease (IBD) is a disabling chronic inflammatory disease of the gastrointestinal tract. IBD patients have increased intestinal lymphatic vessel density and recent studies have shown that this may contribute to the resolution of IBD. However, the molecular mechanisms involved in IBD-associated lymphangiogenesis are still unclear. In this study, we established a novel inflammatory lymphangiogenesis model in zebrafish larvae involving colitogenic challenge stimulated by exposure to 2,4,6-trinitrobenzenesulfonic acid (TNBS) or dextran sodium sulphate (DSS). Treatment with either TNBS or DSS resulted in vascular endothelial growth factor receptor (Vegfr)-dependent lymphangiogenesis in the zebrafish intestine. Reduction of intestinal inflammation by the administration of the IBD therapeutic, 5-aminosalicylic acid, reduced intestinal lymphatic expansion. Zebrafish macrophages express vascular growth factors vegfaa, vegfc and vegfd and chemical ablation of these cells inhibits intestinal lymphatic expansion, suggesting that the recruitment of macrophages to the intestine upon colitogenic challenge is required for intestinal inflammatory lymphangiogenesis. Importantly, this study highlights the potential of zebrafish as an inflammatory lymphangiogenesis model that can be used to investigate the role and mechanism of lymphangiogenesis in inflammatory diseases such as IBD.
Reviews DVD/Blu-ray
Authors: Grasberg Sara , Jeng Jonah , Polisky Laurie 
Published: September 2015

Abstract
We Are What We Are (2013) USA/France Director Jim Mickle Runtime 105 minutes
DVD USA, 2014 Distributed by Entertainment One Film USA LLC (region 1)
The Raid 2 (2014) Indonesia Director Gareth Evans Runtime 150 minutes
DVD USA, 2014 Produced and Distributed by Sony Picture Classics (region 1)
Wadjda (2012) Saudi Arabia Director Haifaa Al-Mansour Runtime 98 minutes
DVD USA, 2014 Produced and Distributed by Sony Picture Classics (region 1)
Crystal structure of diaqua-dinitrato-κ1O-bis(2-(pyridin-4-ylthio)- pyrazine-κ1N)cadmium(II), C18H18CdN8O8S2
Authors: Wang Deng-Wu , Wang Fang 
Published: September 2015

Abstract C18H18CdN8O8S2, monoclinic, P21/c (no. 14), a = 12.9345(5) Å, b = 12.9120(5) Å, c = 7.2991(3) Å, β = 91.085(2)°, V = 1218.8 Å3, Z = 2, Rgt(F) = 0.0401, wRref(F2) = 0.0939, T = 296 K.
Crystal structure of 1-acetylimidazolidine-2,4-dione monohydrate, C5H8N2O4
Authors: Mabkhot Y. N. , Al-Showiman S. S. , Barakat A. , Yousuf S. , Choudhary M. I. 
Published: September 2015

Abstract C5H8N2O4, monoclinic, P21/m (no. 11), a = 6.385(2) Å, b = 6.150(2) Å, c = 8.771(2) Å, β = 105.698(4)°, V = 331.5 Å3, Z = 2, Rgt(F) = 0.0358, wRref(F2) = 0.0955, T = 273 K.
A review of different solvents, mass transfer, and hydrodynamics for postcombustion CO2 capture
Authors: Mirzaei Somayeh , Shamiri Ahmad , Aroua Mohamed Kheireddine 
Published: September 2015

Abstract
There is a growing environmental concern regarding carbon dioxide (CO2) emissions from human activities that result in global warming or climate change. To tackle this potential problem, it is crucial to develop CO2 capture technologies. This paper reviews the current status of postcombustion carbon capture by absorption in packed column using different solvents. The major concerns with the selection of absorbent, such as absorption rate, CO2 absorption capacity, CO2 solubility, environmental cost, and toxicity, are discussed. The hydrodynamics and mass-transfer performance of CO2 absorption in a packed column are reviewed. The determinant factors of CO2 absorption, including effective interfacial area and mass-transfer coefficients in different contactors, are discussed. Liquid holdup and pressure-drop models are investigated.
N-succinyl chitosan preparation, characterization, properties and biomedical applications: a state of the art review
Authors: Bashir Shahid , Teo Yin Yin , Ramesh S. , Ramesh K. , Khan Amir Azam 
Published: September 2015

AbstractN-succinyl chitosan (NSC) remains a promising chitosan derivative to develop targeted drug delivery, wound dressings, and tissue engineering systems. All these systems are important in life sciences. NSC is an amphiprotic derivative obtained from the N-acylation of chitosan. NSC exhibits extraordinary biocompatibility, significantly increased aqueous solubility in acidic and basic media without affecting the biological properties, appreciable transfection efficiency, and the ability to stimulate osteogenesis. NSC shows enhanced bioavailability, which highlights its potential applications in the biomedical field. This review briefly introduces chitosan, including its limitations as a biomaterial, and modifications of chitosan with a particular focus on acylation, along with a comprehensive overview of the synthesis, characterization, properties, biodistribution, and toxicological/biopharmaceutical profile of NSC. Furthermore, it extensively surveys current state-of-the-art NSC-based formulations for drug delivery with special emphasis on protein delivery, anti-cancer activity in the colon, as well as nasal and ophthalmic targeted gene/drug delivery. Moreover, it discusses NSC-based biomaterial applications in articular, adipose, and bone tissue engineering. In addition, it describes recent contributions of NSC-based hydrogels in wound dressings along with a brief account of drug delivery in combination with tissue engineering. Finally, it presents potential current challenges and future perspectives of NSC-based formulations in the biomedical field.
Electromagnetic hypersensitivity – an increasing challenge to the medical profession
Authors: Hedendahl Lena , Carlberg Michael , Hardell Lennart 
Published: September 2015

AbstractBackground:In 1970, a report from the former Soviet Union described the “microwave syndrome” among military personnel, working with radio and radar equipment, who showed symptoms that included fatigue, dizziness, headaches, problems with concentration and memory, and sleep disturbances. Similar symptoms were found in the 1980s among Swedes working in front of cathode ray tube monitors, with symptoms such as flushing, burning, and tingling of the skin, especially on the face, but also headaches, dizziness, tiredness, and photosensitivity. The same symptoms are reported in Finns, with electromagnetic hypersensitivity (EHS) being attributed to exposure to electromagnetic fields (EMF). Of special concern is involuntary exposure to radiofrequency (RF)-EMF from different sources. Most people are unaware of this type of exposure, which has no smell, color, or visibility. There is an increasing concern that wireless use of laptops and iPads in Swedish schools, where some have even abandoned textbooks, will exacerbate the exposure to EMF.Methods:We have surveyed the literature on different aspects of EHS and potential adverse health effects of RF-EMF. This is exemplified by case reports from two students and one teacher who developed symptoms of EHS in schools using Wi-Fi.Results:In population-based surveys, the prevalence of EHS has ranged from 1.5% in Sweden to 13.3% in Taiwan. Provocation studies on EMF have yielded different results, ranging from where people with EHS cannot discriminate between an active RF signal and placebo, to objectively observed changes following exposure in reactions of the pupil, changes in heart rhythm, damage to erythrocytes, and disturbed glucose metabolism in the brain. The two students and the teacher from the case reports showed similar symptoms, while in school environments, as those mentioned above.Discussion:Austria is the only country with a written suggestion to guidelines on the diagnosis and treatment of EMF-related health problems. Apart from this, EHS is not recognized as a specific diagnosis in the rest of the world, and no established treatment exists.Conclusion:It seems necessary to give an International Classification of Diseases to EHS to get it accepted as EMF-related health problems. The increasing exposure to RF-EMF in schools is of great concern and needs better attention. Longer-term health effects are unknown. Parents, teachers, and school boards have the responsibility to protect children from unnecessary exposure.
Investigation of HP Turbine Blade Failure in a Military Turbofan Engine
Authors: Mishra R. K. , Thomas Johny , Srinivasan K. , Nandi Vaishakhi , Bhatt R. Raghavendra 
Published: September 2015

AbstractFailure of a high pressure (HP) turbine blade in a military turbofan engine is investigated to determine the root cause of failure. Forensic and metallurgical investigations are carried out on the affected blades. The loss of coating and the presence of heavily oxidized intergranular fracture features including substrate material aging and airfoil curling in the trailing edge of a representative blade indicate that the coating is not providing adequate oxidation protection and the blade material substrate is not suitable for the application at hand. Coating spallation followed by substrate oxidation and aging leading to intergranular cracking and localized trailing edge curling is the root cause of the blade failure. The remaining portion of the blade fracture surface showed ductile overload features in the final failure. The damage observed in downstream components is due to secondary effects.
Proposal of a clinical response score and predictors of clinical response to 2 years of GH replacement therapy in adult GH deficiency
Authors: Schneider Harald J , Buchfelder Michael , Wallaschofski Henri , Luger Anton , Johannsson Gudmundur , Kann Peter H , Mattsson Anders 
Published: September 2015

ObjectiveThere is no single clinical marker to reliably assess the clinical response to growth hormone replacement therapy (GHRT) in adults with growth hormone deficiency (GHD). The objective of this study was to propose a clinical response score to GHRT in adult GHD and to establish clinical factors that predict clinical response.DesignThis was a prospective observational cohort study from the international KIMS database (Pfizer International Metabolic Database).MethodsWe included 3612 adult patients with GHD for proposing the response score and 844 patients for assessing predictors of response. We propose a clinical response score based on changes in total cholesterol, waist circumference and QoL-AGHDA quality of life measurements after 2 years of GHRT. A score point was added for each quintile of change in each variable, resulting in a sum score ranging from 3 to 15. For clinical response at 2 years, we analysed predictors at baseline and after 6 months using logistic regression analyses.ResultsIn a baseline prediction model, IGF1, QoL-AGHDA, total cholesterol and waist circumference predicted response, with worse baseline parameters being associated with a favourable response (AUC 0.736). In a combined baseline and 6-month prediction model, baseline QoL-AGHDA, total cholesterol and waist circumference, and 6-month change in waist circumference were significant predictors of response (AUC 0.815).ConclusionsA simple clinical response score might be helpful in evaluating the success of GHRT. The baseline prediction model may aid in the decision to initiate GHRT and the combined prediction model may be helpful in the decision to continue GHRT.
Effects of Genotype and Sleep on Temperament
Authors: Bouvette-Turcot Andrée-Anne , Pluess Michael , Bernier Annie , Pennestri Marie-Hélène , Levitan Robert , Sokolowski Marla B. , Kennedy James L. , Minde Klaus , Steiner Meir , Pokhvisneva Irina , Meaney Michael J. , Gaudreau Hélène 
Published: September 2015

BACKGROUND AND OBJECTIVES:Sleep problems are frequent in young children; however, children vary in the degree to which they are affected by poor sleep quality. We investigated whether a polymorphism in the serotonin transporter gene, which is linked to emotional function, is a potential moderator of the influences of sleep duration on infant temperament using longitudinal data.METHODS:We examined the interactive effects of average sleep duration between 6 and 36 months of age and the 5-HTTLPR genotype on negative emotionality/behavioral dysregulation at 36 months in 209 children recruited into a longitudinal birth cohort study. Triallelic genotyping of 5-HTTLPR was performed by looking at SLC6A4 genotype, focusing on the serotonin transporter-linked polymorphic region (5-HTTLPR) including the SNP polymorphism (rs23351). Child sleep habits were assessed with a maternal self-report questionnaire.RESULTS:After controlling for demographics and both previous and concurrent maternal depression, multiple linear regression analyses revealed a significant interaction effect of average sleep duration for the first 3 years of life and 5-HTTLPR genotype on child negative emotionality/behavioral dysregulation such that the effects were exclusive to those with low-expressing 5-HTTLPR genotypes.CONCLUSIONS:The results suggest differential susceptibility to the effect of sleep duration early in life, which reiterates that the short allele of the 5-HTTLPR represents a marker of increased environmental sensitivity regarding emotional development. Differential susceptibility theory posits that certain factors may increase an individual’s susceptibility to the environment, in either a positive or negative fashion.
FERNANDO DO Ó E A EDUCAÇÃO: CONEXÕES COM O CAMPO RELIGIOSO DE SANTA MARIA * FERNANDO DO Ó AND EDUCACITION: CONNECTION WITH THE RELIGIOUS FIELD OF SANTA MARIA
Authors: Mattos Renan 
Published: September 2015

<div><p><strong>Resumo</strong>: O campo educacional foi considerado como primordial nas disputas religiosas e filosóficas do final do século XIX e meados do século XX. Partindo dos conceitos de campo religioso e campo intelectual de Pierre Bourdieu, temos por tema de pesquisa a trajetória de Fernando do Ó e sua relação com o movimento espírita . Nesse sentido, o presente artigo analisa a proposta educacional de Fernando do Ó veiculada no jornal <em>Diário do Interior </em>no recorte de 1930 a 1940. Entende-se que tais posicionamentos permitem refletir tanto suas interpretações da sociedade quanto as concorrências existentes em tal contexto social.</p><p><strong>Palavras-chave: </strong>Educação; Espiritismo; Santa Maria; Era Vargas.</p><p><strong>Abstract: </strong>The educational field was considered paramount in religious and philosophical disputes of the late nineteenth and mid -twentieth century. Based on the concepts of the religious field and field of intellectual Pierre Bourdieu, we analyze topics for the trajectory of Fernando do Ó and his relationship with the spiritualist movement . In this sense, this paper analyzes the educational proposal of Fernando do Ó conveyed in the newspaper <em>Diário do Interior </em>cutout in 1930-1940. It is understood that such placements allow reflect both their interpretations of society as existing in such competitions social context.</p><p><strong>Keywords: </strong>Education; Spiritism; Santa Maria; Era Vargas. </p></div>
Parasites destabilize host populations by shifting stage‐structured interactions
Authors: Hite Jessica L. , Penczykowski Rachel M. , Shocket Marta S. , Strauss Alexander T. , Orlando Paul A. , Duffy Meghan A. , Cáceres Carla E. , Hall Spencer R. 
Published: September 2015

AbstractShould parasites stabilize or destabilize consumer–resource dynamics? Recent theory suggests that parasite‐enhanced mortality may confer underappreciated stability to their hosts. We tested this hypothesis using disease in zooplankton. Across both natural and experimental epidemics, bigger epidemics correlated with larger—not smaller—host fluctuations. Thus, we tested two mechanistic hypotheses to explain destabilization or apparent destabilization by parasites. First, enrichment could, in principle, simultaneously enhance both instability and disease prevalence. In natural epidemics, destabilization was correlated with enrichment (indexed by total phosphorous). However, an in situ (lake enclosure) experiment did not support these links. Instead, field and experimental results point to a novel destabilizing mechanism involving host stage structure. Epidemics pushed hosts from relatively more stable host dynamics with less‐synchronized juveniles and adults to less stable dynamics with more‐synchronized juveniles and adults. Our results demonstrate how links between host stage structure and disease can shape host/consumer–resource stability.