bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultivar-specific wheat-associated bacterial communities and metabolites in response to nitrogen deficiency
Authors: Chan Lok Hang , Lam Shu Kee , Chen Deli , Tang Caixian , Chen Qinglin , Roessner Ute , Salazar Vinícius Werneck , Gupta Sneha , Dias Daniel Anthony , Hu Hang-Wei 
Published: August 2024

Abstract

Background and Aims
Nitrogen (N) deficiency in soil constrains plant growth, which may potentially be alleviated by beneficial soil bacterial communities. However, there is limited knowledge of the plant-bacteria interactions of wheat cultivars with different N-use efficiency (NUE) under N deficiency.
Methods
We investigated the responses of soil and root endosphere bacterial communities as well as root metabolites of two wheat cultivars (cv. Mace and Gladius) with reported high and low NUE, respectively, using a glasshouse experiment and a hydroponic experiment with three N levels.
Results
The rhizosphere bacterial community of Mace shifted under N deficiency, but not in its root endosphere. Conversely, the rhizosphere bacterial community of Gladius remained unchanged under N deficiency but shifted in its root endosphere. The metagenomic analysis illustrated increased detection of genes related to bacterial growth and motility in the rhizosphere of Mace, but not of Gladius, under N deficiency. A 4-fold increase in octadecanoic acid in the root of Mace, but not Gladius, under N deficiency, suggesting the potential role of octadecanoic acid in shaping the rhizobacterial community in Mace with higher reported NUE.
Conclusion
Our study highlights the divergent responses of wheat-associated microorganisms and root metabolites to N deficiency in the two cultivars. We found that wheat cultivars with higher NUE increased octadecanoic acid secretion, which potentially shaped the rhizobacterial communities, thereby enhancing their growth under N-limited conditions.
Problematic Internet Use Profiles: Identifying Social-Cultural Demographic Risk Factors and Predicting Longitudinal Effects on Psychopathology
Authors: Wang Yi , Hall Brian , Chen Yuran , Chen Chun 
Published: August 2024

Abstract

Background
Problematic Internet Use (PIU) has many adverse effects on youth mental health and development. However, few studies have systematically investigated the internal heterogeneity of PIU symptoms among rural Chinese adolescents. This study collected two waves of data (T1: October 2022; T2: April 2023) from 5,271 rural Chinese adolescents from two secondary schools in Guizhou and Sichuan provinces.
Methods
A Latent Profile Analysis (LPA) was conducted to first identify PIU symptom profiles. Then, a “three-step” logistic regression mixed model was conducted to explore the association between PIU patterns and demographic correlates. Anxiety, depression, and stress symptoms collected at the second wave were compared across PIU profiles.
Results
The study found that (1) The patterns of PIU among rural adolescents could be divided into four subgroups: low PIU group (57.18%), medium PIU group (15.65%), high PIU group (9.01%), and self-blame group (18.16%), which is a uniquely identified group. (2) Being female, an ethnic minority, living off-campus, having left-behind experiences, and having fewer siblings were risk factors for high PIU group membership. (3) The order of severity for anxiety, depression, and stress was as follows: high PIU, medium PIU, self-blame, and low PIU groups.
Conclusions
Addressing the dimension of internet obsession of PIU is vital for rural Chinese adolescent mental health, necessitating tailored interventions involving families and schools.
Short Term Oxidation in HT-SEM of the Pt-containing TROPEA Single Crystal Ni-based Superalloy from 680 to 1000°C
Authors: Mathieu S. , Podor R. , Emo M. , Hunault L. , Vilasi M. , Cormier J. , Pedraza F. 
Published: August 2024

Abstract

Short-time oxidation of the Ni-based TROPEA single-crystal superalloy was implemented to determine the nature and quantities of transient oxides in the 680–1000°C temperature range. Experiments were carried out in situ in the SEM with reduced air pressure (150 Pa, \(\:{P}_{{O}_{2}}\) ∼ 31.5 Pa) compared to atmospheric conditions (105 Pa, \(\:{P}_{{O}_{2}}\) ∼ 2.1 104 Pa). TEM characterization after oxidation showed the complexity of the oxidation products developed. Aluminum underwent internal oxidation between 680 and 1000°C. During the limited duration of oxidation, the TROPEA alloy only formed a continuous alumina layer at 1000°C. At 680 and 850°C, the low diffusion rate and small amount of Al in the Ni-based single crystal led to the formation of a significant amount of transient oxides such as (Ni,Co)O, compared to the desired chromia or alumina protective oxides. The lower the temperature, the smaller the size of the internal Al2O3 precipitates and the larger the transient oxide crystals, which would lower the resistance of TROPEA to Type II hot corrosion. In contrast after a transient period shorter than 22 hours, during which multiple transient oxide developed, the oxidation resistance would be ensured at 1000°C by the formation of a continuous Al2O3 scale.
Comparative functional analysis of a new CDR1-like ABC transporter gene in multidrug resistance and virulence between Magnaporthe oryzae and Trichophyton mentagrophytes
Authors: Wang Jing , Xiao Chenwen , Liang Shuang , Noman Muhammad , Cai Yingying , Zhang Zhen , Zhu Xueming , Chai Rongyao , Qiu Haiping , Hao Zhongna , Wang Yanli , Wang Jiaoyu , Bao Guolian , Sun Guochang , Lin Fucheng 
Published: August 2024

Abstract

Fungi are notorious for causing diseases in plants and domestic animals. ABC transporters play pivotal roles in multidrug resistance in fungi, with some ABC proteins indispensable for the pathogenicity of plant fungal pathongens. However, the roles of ABC proteins in animal pathogenic fungi, and the functional connections between ABC homologues in plant and animal pathogenic fungi are largely obscure. Here, we first functionally compared ABCG-1 proteins in rice-blast fungus Magnaporthe oryzae and identified MoCDR1, a new ABC gene invovled in pathogencity. MoCDR1 disruption caused hypersensitivity to multidrugs, and impaired conidiation, appressorium formation and pathogenicity. Subsequently, we systematically retrieved ABC proteins in animal pathogenic fungus Trichophyton mentagrophytes and identified TmCdr1, a homologue to MoCdr1. TmCDR1 effectively rescued the drug sensitivity and virulece of ΔMocdr1, and mediated the drug resistance in T. mentagrophytes. TmCDR1 deletion did not significantly weaken the lesions on animal skin, but the mutant elicited a lower response on mouse macrophages than the wild type. Moreover, MoCdr1 and TmCdr1 are conserved in structures and functions, and both involved in drug resistance and pathogenicity by analogously regulating gene expression levels related to transporter activity, MAPK signaling pathway, and metabolic processes. Altogether, our results represents the first comprehensive characterization of ABC genes in T. mentagrophytes, establishing a functional correlation between homologous ABC genes in plant and animal pathogenic fungi.
Ageing is associated with a decline in the complexity of isometric precision pinch grip force and manual dexterity
Authors: Mauger Alexis R. , Hopker James G. , Fennell Christopher RJ. 
Published: August 2024

Abstract

Introduction
The study investigated the time-course of age-related changes in the complexity of isometric precision pinch grip (PG) force and the performance of manual dexterity tasks.
Methods
Sixty-four individuals across four age groups, younger group (YG; 18 to 30 years), 50 year group (50yr; 50 to 59 years), 60 year group (60yr; 60 to 69 years) and 70 year group (70yr; 70 to 85 years) participated in the study. Participants performed submaximal constant intensity isometric precision PG force control tasks. The complexity of the isometric force signals was assessed using the multiscale entropy analysis (MSE) and detrended fluctuation analysis (DFA). Participants also completed the Purdue Pegboard and Moberg pick-up manual dexterity tests.
Results
There was a main effect of age group on DFA (P = 0.005) and MSE (P < 0.001). The 70 yr group presented with a higher DFA and lower MSE, compared with the YG, 50 yr and 60 yr group (all P < 0.001). The 70 yr group performed worse at the Purdue Pegboard and Moberg pick-up test compared with the YG, 50 yr and 60 yr group (all P < 0.05).
Conclusion
The current study found that beyond 70 years of age there is a significant decline in isometric precision PG force signal complexity and manual dexterity, when compared with adults under 70 years of age. Collectively, study findings are indicative of preserved fine motor control into the 6th and 7th decades of life, with a functional decline in fine motor control in the 8th decade.
DAAO mutant sites among different mice strains and their effects on enzyme activity
Authors: Yu-cong Zhou , Sheng-ling Fu , Jun-xuan Yang , Hao Liu 
Published: August 2024

Abstract

Previous studies reported that the up-regulation of DAAO expression and enzyme activity levels were closely related to the development of neuropathic pain, cognitive characteristics of schizophrenia and so on. To determine DAAO mutant sites in different strains of mice and their effects on enzyme activity, we successfully constructed a prokaryotic expression system for heterologous expression of DAAO in vitro. There were total five nucleotide mutations distributed in exons 2, 8, 9, 10 of C57 mice. Three mutations located on exons 8 and 9 were synonymous mutations and had no variation on the encoded amino acid. The remaining two mutations in exons 2 (V64A) and 10 (R296H) were non-synonymous mutations, which might affect enzymatic activity and protein structure of mDAAO. Based on the determination of the kinetic constants and IC50 of mDAAO mutants in vitro, the differences in amino acid levels at these two sites (V64A, R295H) partly impacted the catalytic efficiency of DAAO mutants and their affinity with some inhibitors in vitro.
Comparison of Tumor Markers and Risk of Malignancy Index (RMI) in Borderline OvarianTumors Names of
Authors: Demircivi Ergul , Donmez Nisan Helin , Callioglu Nihal , Turgut Abdulkadir 
Published: August 2024

Abstract

Background
Borderline ovarian tumours (BOTs) are non-invasive tumours with low malignancy potential frequently observed in patients of reproductive age. Therefore, preoperative differential diagnosis is important in these patients. In our study, we tried to differentiate benign and borderline ovarian tumours preoperatively using risk of malignancy (RMI) index and tumour markers.
Methods
In our study, we retrospectively compared tumour markers and risk of malignancy index (RMI) of 85 patients aged between 17–84 years with postoperative benign (n:52) and borderline (n:33) ovarian cysts.
Results
In our study, the mean age of the benign group was significantly higher than the borderline group (p = 0.001). Ca 125 and RMI values were significantly higher in the borderline group compared to the benign group (p = 0.001 and p = 0.018). In addition, mucinous tumours had significantly larger tumour diameter than serous tumours in the borderline group (p = 0.022).
Conclusion
As a result of our study, since borderline ovarian tumours are seen in young patients of reproductive age, it may be suggested to use Ca125 and RMI for the differential diagnosis of benign and borderline cysts preoperatively.
Multiple misdiagnoses of paraspinal synovial sarcoma as tuberculosis: A case report and literature review
Authors: Liu Jie , Huang Xiajie , Liang Xinyun , Xian Xinhua , Mo Yangzhou , Wu Xiaomei , Lu William W , Li Jian , Chen Yan 
Published: August 2024

Abstract

Background Synovial sarcoma (SS) is a rare but aggressive soft tissue sarcoma that can be challenging to diagnose accurately, especially when it mimics more common conditions such as tuberculosis. This case report aims to highlight the diagnostic difficulties and emphasize the importance of early and accurate identification.Case Presentation A 23-year-old female patient presented with left-sided lumbar pain that progressively worsened over three weeks, accompanied by weakness and numbness in the left lower limb. She was misdiagnosed twice by two hospitals as having a spinal tuberculous abscess based on weakly positive anti-tuberculosis antibody and imaging findings. Despite continuous anti-tuberculosis treatment, her condition deteriorated. The first surgery revealed findings inconsistent with spinal tuberculosis, and a tumor could not be ruled out, but the pathological biopsy was inconclusive. A second surgery was performed to resect the mass and obtain a biopsy, which confirmed SS. Unfortunately, by this time, the disease had metastasized to her lungs, and she missed the optimal window for surgical intervention.Conclusion This case highlights the necessity of considering malignancies such as SS in the differential diagnosis of spinal lesions, especially when the clinical response to treatment is poor. Early diagnosis and intervention are crucial for improving patient outcomes. Our literature review thoroughly examines the characteristics of paraspinal SS and provides insights to prevent misdiagnosis. It emphasizes the importance of timely surgical intervention to improve patient survival.
Taurine Alleviated Acrylamide-Induced Ovarian Toxicity via Suppression of Stress and Apoptosis in Mice
Authors: Khodabandeh Zahra , Alaee Sanaz , Samare-Najaf Mohammad , Hosseini Elham , Dara Mahintaj , Shokri Saeed , Shirazi Reza 
Published: August 2024

Abstract

Acrylamide (Acr) poses reproductive toxicity risks to humans due to its ability to penetrate cell membranes and disrupt cellular balance. Taurine (Tau), a sulfur-containing amino acid with cell membrane stabilization and antioxidant properties, may mitigate these effects. This study examines how Tau can protect against oxidative stress and apoptosis induced by Acr in mouse ovarian tissue. Forty adult healthy mice, aged 6-8 weeks, were randomly assigned to four including Con (received normal saline orally), Acr (50 mg/Kg/day Acr orally), Acr+Tau75 (Acr and 75 mg/Kg/day Tau), and Acr+Tau150 (Acr and 150 mg/Kg/day Tau). Treatments were administered for 35 days, followed by assessments of stress markers and apoptosis via immunofluorescence and TUNEL assays. Both doses of Tau significantly enhanced the gene and protein expression levels of stress response enzymes, including GPx, SOD, and CAT (p <0.05). Moreover, Tau significantly decreased the gene expression levels of apoptotic markers Caspase3 and BAX, while upregulating the anti-apoptotic gene BCL2L1 (p < 0.01). The TUNEL assay revealed the ameliorative properties of Tau against Acr-induced apoptosis in the ovaries. The current findings suggest the promising properties of Tau in the amelioration of Acr-induced oxidative stress and apoptosis in mouse ovarian tissue. Therefore, Tau presents a potential therapeutic approach for protecting against Acr-induced reproductive toxicity in females, warranting further investigation.
Gloveport-assisted retroperitoneal pyeloplasty (GARP): a case report
Authors: Montero Javier Arredondo , Ruiz María Rodríguez 
Published: August 2024

Abstract

Single-port video-assisted techniques, such as one-trocar-assisted pyeloplasty (OTAP) or Round-Traction-Assisted Pyeloplasty (RoTAP), have proven helpful for correcting congenital ureteropelvic junction obstruction (UPJO), especially in infants. We present a case report of a patient diagnosed with UPJO who underwent a minimally invasive pyeloplasty with a RoTAP technical variation based on the gloveport system that allowed bimanual endocavitary dissection and protected the surgical wound during the procedure without increasing the cost or technical difficulty compared to the original technique. This new technique, called gloveport-assisted retroperitoneal pyeloplasty (GARP), could be a valuable alternative for this pathology.
Fully proximal migrated double J-stent in a pediatric patient with congenital ureteropelvic junction obstruction: a pediatric case report
Authors: Montero Javier Arredondo , Ruiz María Rodríguez 
Published: August 2024

Abstract

Proximal double-J stents (DJ) migration is infrequent in the pediatric population. We present the case of a 5-year-old patient with a previous diagnosis of ureteropelvic junction obstruction who underwent a dismembered pyeloplasty in early infancy and suffered an anastomotic stricture. Endourological dilatation was performed. During placement of the DJ, it migrated proximally until it was wholly lodged in the renal pelvis. A second DJ was placed correctly, and the procedure was ended. Two months later, a successful retrograde removal was performed using an endourological hydrodistension system. We describe our technique, which may be a valuable resource for similar cases.
Assessment of Heavy Metals in Vegetables Grown on Irrigated Land in Butura, Bokkos LGA, Plateau State, Nigeria
Authors: Solomon  
Published: August 2024

Abstract

Vegetables have positive antioxidative properties and are abundant in vitamins, minerals, and fiber. However, if consumed in large quantities, eating vegetables polluted with heavy metals may be harmful to human health. Therefore, this study assessed the effects of heavy metals on irrigated pepper, cabbage and Irish potatoes grown in Butura. Atomic absorption spectrophotometry (AA240FS) was used to analyze cadmium (Cd), cobalt (Co), nickel (Ni), lead (Pb), zinc (Zn), copper (Cu), chromium (Cr) and arsenic (As) levels. Three samples were selected from each of the vegetables grown on nine selected farms at distances of 0 m, 10 m, and 30 m. This forms a composite sample of vegetables at each farm. The study showed that the concentrations of cobalt, chromium, cadmium, copper, arsenic, zinc and nickel were within the standard limits set by the FAO/WHO, except for lead, which is higher than the allowable limits for vegetables. These patients may have behavioral problems, neurological complications and hematologic disorders. Thus, these findings could lead to a risk for the human population consuming these vegetables. It is recommended that irrigation water and agricultural soils be constantly monitored to determine the concentration of metals accumulated by crop plants to ensure that crop plants are safe for consumption by humans.
Knowledge, Attitudes, and Practices toward Physical Activity among Public Sector Librarians in Brunei Darussalam
Authors: Tuah Nik A A , Rahman Amirul , Rahman Hanif Abdul 
Published: August 2024

Abstract

One in four adults globally has an insufficient level of physical activity (PA). Librarians, due to the sedentary nature of their profession, are at risk. This study aimed to assess the knowledge, attitudes, and practices toward PA among public sector librarians in Brunei Darussalam. A cross-sectional study was conducted using a validated questionnaire. A total of 194 participants completed the survey (71.85%). The findings revealed good knowledge (mean 8.71, SD 1.12), poor attitudes (mean 35.63, SD 3.97), and satisfactory practices (mean 23.49, SD 3.18) toward PA. Multiple linear regression revealed several key findings. Librarians with higher education had higher knowledge scores (P = 0.046, t = 2.01, 95% CI: 0.01, 0.64) than did those who completed secondary education, and librarians who did not engage in PA (P = 0.009, t = -2.64, 95% CI: 2.64) had lower knowledge scores than did those who engaged in PA. Compared with their male counterparts. female librarians had lower attitude scores (P = 0.003, t = -3.00, 95% CI: -3.84, -0.79). Older age groups 40–49 years (P = 0.014, t = 1.62, 95% CI: -0.24, 3.41) and 50–60 years (P = 0.023, t = 2.46, 95% CI: 0.34, 3.14) had higher practice scores than did the other age groups, and librarians who did not engage in PA tended to have lower practice scores than did those who did not (P = 0.000, t = -3.52, 95% CI: -2.81, -0.79). Future research and policy could focus on developing tailored interventions and wellness programme for PAs.
Investigation of in vivo toxicity and biochemical perturbation of plasticizers identified in the Tunisian sweets and the fate in WWTP and adjacent coastal area
Authors: Belaid Afifa , Bekir Karima , Beltifa Asma , Ben Mansour Hedi 
Published: August 2024

ABSTRACT

Plasticizers have been excessively used in food packaging and numerous studies show their migration into food and their fate in the environment after the release of plastic packaging into the receiving environment. Our results show the sweets products analyzed using GC-MS/MS indicated the presence of benzyl butyl phthalate (BBP), dibutyl phthalate (DBP), bis(2-ethylhexyl) phthalate (DEHP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), and 1,2-cyclohexane dicarboxylic acid diisononyl ester (DINC). Slightly or not metabolized in the body, plasticizers can reach WWTPs and contaminated wastewater. Generally, the usual treatment procedures in WWTPs are not effective in removing these plasticizers, and therefore, they end up in the sea and concentrate in the sediments. This is what our study showed by detecting plasticizers at high concentrations such as DINB which reached 0.93 mg/L in the tested WWTP and 3.8 mg/kg in the sediment of the adjacent marine coast. These analyses generally have negative effects on human health. In fact, we have tested the toxicity of the main detected phthalates such as DINP, DEHP, and DBP. The results showed that the acute administration significantly induced liver and kidney injuries in male mice manifested by a rise in plasma uric acid, alanine aminotransferase, and glucose.
Industrial Application of Hydrotalcite-Based Materials
Authors: Banik Nibedita , Kaur Navdeep 
Published: August 2024

Hydrotalcite is a layered double hydroxide (LDH) with a wide range of
applications in catalysis, flame retardancy, PVC stabilization, fillers and
reinforcements, additives in coatings and paints, pharmaceuticals and personal care,
environmental remediation, and other industrial applications. This paper provides an
overview of the properties and characteristics of hydrotalcite, as well as its potential
applications in various industries. The paper begins with an introduction to hydrotalcite, including its definition,
structure, synthesis methods, and properties. The next section discusses the role of
hydrotalcite as a catalyst, as well as its applications in the petrochemical industry and
environmental remediation. The following section focuses on the flame retardant and
smoke suppression properties of hydrotalcite, and its applications in plastics and
polymers. The subsequent section discusses the use of hydrotalcite as a PVC stabilizer
and its benefits and applications in the PVC industry. The following sections discuss the use of hydrotalcite as a filler and reinforcement in
composite materials, as an additive in coatings and paints, and in pharmaceutical and
personal care formulations. The next section discusses the applications of hydrotalcite
in water treatment, soil remediation, and battery and energy storage. The final section
summarizes the future prospects and challenges of hydrotalcite, including emerging
research areas, challenges and limitations, and potential for further industrial
applications.This paper provides a comprehensive overview of the properties and applications of
hydrotalcite. It is intended to be a valuable resource for researchers, engineers, and
other professionals who are interested in this versatile material.