bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of extruded polystyrene bead foams via supercritical CO2 and secondary foaming technology
Authors: Luo Haibin , Su Yaozhuo , Huang Pengke , Zheng Hao , Zhang Lulu , Wu Fei , Zhao Yongqing , Wu Xingyu , Zheng Wenge 
Published: July 2024

AbstractBead foaming is a burgeoning technology for manufacturing polymer foams with lightweight and complex geometrical structures, and one of the representative cases is polystyrene (PS) bead foams. Here, a facile method is proposed to manufacture extruded PS bead foams via supercritical CO2 extrusion foaming. Secondary foaming is introduced to improve the expansion ratio of extruded bead foams and to reveal their bead bonding mechanism. For example, the expansion ratio of extruded bead foams can be increased from 21‐fold to 40‐fold after secondary foaming, illustrating that extruded bead foams have excellent secondary growth ability. In addition, the effect of molding pressure on the interface bonding of bead foams is also studied. It can be seen that there is no obvious gap between the extruded bead foams, even better than the commodity foamed boards. Benefitting from the excellent bonding effect, the as‐prepared foamed boards exhibit outstanding mechanical properties.
Unveiling the catalytic potential of K2ZnF6 fluoroperovskite for glycerol oligomerization
Authors: Barros Fernando José Soares , Moreno‐Tost Ramón , Cecilia Juan A. , de Luna Francisco Murilo Tavares , Rodríguez‐Castellón Enrique , Vieira Rodrigo Silveira 
Published: July 2024

AbstractThis study systematically evaluates fluorine‐containing materials with varying compositions of K, Al, Co, Cu, Mg, and Zn as catalysts for glycerol oligomerization. Comprehensive analyses were conducted for all of the catalysts, considering their structural, compositional, morphological characteristics, and basicity, to understand them in greater depth. Among these analyses, gas chromatography was employed to examine the composition of the reaction products. The investigation extended to a recycling study under optimized conditions. Thermogravimetric analysis and X‐ray photoelectron spectroscopy were used to characterize the spent catalysts. Catalytic activity was found to be influenced strongly by the fluorine content, demonstrating remarkable performance for the materials containing fluoroperovskites such as KMgF3 in the catalyst KMgAFC, and K2ZnF6 in KZAFC. Remarkably, KZAFC with 20% fluorine exhibited superior glycerol conversion (68%) and selectivity for diglycerol (27%). Comparative analysis with ZnAl‐layered double hydroxide highlighted the positive impact of fluorine on catalytic activity. Introducing a calcination step in the catalyst recovery process also demonstrated improved activity during recycling. Interestingly, KZAFC exhibited stability over four reaction cycles, presenting an economic advantage over homogeneous catalysts. The study also shed light on the potential fluorine loss at the surface and potassium leaching into reaction products, correlating these phenomena with the observed decline in activity. These comprehensive findings contribute significantly to advancing sustainable glycerol valorization through heterogeneous catalysis.
An engineered ultrahigh affinity bi‐paratopic uPAR targeting agent confers enhanced tumor targeting
Authors: Cherf Gerald M. , Lee Robert B. , Mehta Nishant , Clifford Claire , Torres Kathleen , Kintzing James R. , Cochran Jennifer R. 
Published: July 2024

AbstractUrokinase‐type plasminogen activator receptor (uPAR) is overexpressed on tumor cells in multiple types of cancer and contributes to disease progression and metastasis. In this work, we engineered a novel bi‐paratopic uPAR targeting agent by fusing the binding domains of two native uPAR ligands: uPA and vitronectin, with a flexible peptide linker. The linker length was optimized to facilitate simultaneous engagement of both domains to their adjacent epitopes on uPAR, resulting in a high affinity and avid binding interaction. Furthermore, the individual domains were affinity‐matured using yeast surface display and directed evolution, resulting in a bi‐paratopic protein with affinity in the picomolar to femtomolar range. This engineered uPAR targeting agent demonstrated significantly enhanced tumor localization in mouse tumor models compared to the native uPAR ligand and warrants further investigation as a diagnostic and therapeutic agent for cancer.
Is conventional functional liver remnant volume higher than 40% still sufficient to prevent post‐hepatectomy liver failure in jaundiced patients with hilar cholangiocarcinoma? A single‐center experience in China
Authors: Lv Tian‐Run , Ma Wen‐Jie , Liu Fei , Hu Hai‐Jie , Jin Yan‐Wen , Li Fu‐Yu 
Published: July 2024

AbstractObjectiveOur study aims to evaluate the predictive accuracy of functional liver remnant volume (FLRV) in post‐hepatectomy liver failure (PHLF) among surgically‐treated jaundiced patients with hilar cholangiocarcinoma (HCCA).MethodsWe retrospectively reviewed surgically‐treated jaundiced patients with HCCA between June, 2000 and June, 2018. The correlation between FRLV and PHLF were analyzed. The optimal cut off value of FLRV in jaundiced HCCA patients was also identified and its impact was furtherly evaluated.ResultsA total of 224 jaundiced HCCA patients who received a standard curative resection (43 patients developed PHLF) were identified. Patients with PHLF shared more aggressive clinic‐pathological features and were generally in a more advanced stage than those without PHLF. An obvious inconsistent distribution of FLRV in patients with PHLF and those without PHLF were detected. FLRV (continuous data) had a high predictive accuracy in PHLF. The newly‐acquired cut off value (FLRV = 53.5%, sensitivity = 81.22%, specificity = 81.4%) showed a significantly higher predictive accuracy than conventional FLRV cut off value (AUC: 0.81 vs. 0.60, p < 0.05). Moreover, patients with FLRV lower than 53.5% also shared a significantly higher major morbidity rate as well as a worse prognosis, which were not detected for FLRV of 40%.ConclusionFor jaundiced patients with HCCA, a modified FLRV of 53.5% is recommended due to its great impact on PHLF, as well as its correlation with postoperative major morbidities as well as overall prognosis, which might help clinicians to stratify patients with different therapeutic regimes and outcomes. Future multi‐center studies for training and validation are required for further validation.
Linguistic isolation correlates with length of stay and mortality for pediatric oncology patients in California
Authors: Ennett Samuel , Das Akansha , Burcham Megan , Fitzgerald Robert , Boville Brian , Rajasekaran Surender , Kortz Teresa , Leimanis‐Laurens Mara L. 
Published: July 2024

AbstractObjectiveTo evaluate social drivers of health and how they impact pediatric oncology patients' clinical outcomes during pediatric intensive care unit (PICU) admission via correlation with patient ZIP codes.MethodsDemographic, clinical, and outcome variables from Virtual Pediatric Systems®, LLC for oncology patients (2009–2021) in California PICUs (excluding postoperative) using 3‐digit ZIP Codes with social drivers of health variables linguistic isolation, poverty, race/ethnicity, and education abstracted from American Community Survey data for 3‐digit ZIP Codes using the Environmental Protection Agency's EJScreen tool. Outcomes of length of stay (LOS), mortality, acuity scores, were compared with social variables.ResultsPositive correlation between mortality and minority racial groups (Hispanic/Latino) across ZIP Codes (correlation coefficients of 0.45 (95% CI: 0.22–0.64, p < 0.001) in 2017, 0.50 (95% CI: 0.27–0.68, p < 0.001) in 2018, 0.33 (95% CI: 0.07–0.54, p = 0.013) in 2020, and 0.32 (95% CI: 0.06–0.53, p = 0.018) in 2021). Median PICU length of stay significantly correlated with linguistic isolation (coefficient of 0.42 (95% CI: 0.18–0.61, p = 0.001) in 2021 versus −0.41 (95% CI: −0.61 to −0.16, p = 0.002) in 2019), which included PRISMIII (n = 7417). Mixed effects logistic regression model for other constant variables (PRISMIII, cancer type, race/ethnicity, year), random effect of patient, linguistic isolation (percentage as a continuous value) was significantly associated (95% CI: 1.01–1.06; p = 0.02) with mortality; (OR = 1.03).ConclusionsLinguistic isolation was correlated with LOS and mortality, however variable year to year.
Perspective of obstetric care‐providers on being involved in cervical cancer screening during antenatal care in the Netherlands
Authors: van der Hoeven Nick M. A. , van den Brule A. J. C. , van Beekhuizen H. J. , de Kok I. M. C. M. , van Kemenade F. J. 
Published: July 2024

AbstractBackgroundThe aim of this study was to determine attitude of Dutch midwifes, gynecologists and general practitioners (GPs) towards involvement in antenatal cervical cancer screening (CCS) in the Netherlands.MethodsIn 2021, Dutch midwives, gynecologists, and GPs were offered a single digital questionnaire assessing perceived feasibility, benefits, and harms of antenatal CCS.ResultsA total of 6943 Questionnaires were send and response rate was 18% (N = 1260). Of all respondents, 78% considered antenatal CCS via obstetric care providers feasible. Most respondents (85%) agreed that offering CCS in person can increase motivation to attend. Most midwives (93%) considered that women would feel less encumbered if cervical sampling would be performed by obstetric care providers, rather than by GPs.ConclusionResults indicate that introduction of antenatal CCS is considered feasible by a majority of Dutch midwifes, gynecologists, and GPs. Considered benefits include improved motivation to attend and reduced test related barriers.
METTL1/FOXM1 promotes lung adenocarcinoma progression and gefitinib resistance by inhibiting PTPN13 expression
Authors: Peng Wei , Fu Jia , Zhou Lijun , Duan Huaxin 
Published: July 2024

AbstractIntroductionLung adenocarcinoma (LUAD) is the most common malignant tumor in respiratory system. Methyltransferase‐like 1 (METTL1) is a driver of m7G modification in mRNA. This study aimed to demonstrate the role of METTL1 in the proliferation, invasion and Gefitinib‐resistance of LUAD.MethodsPublic datasets were downloaded from the Gene Expression Profiling Interactive Analysis (GEPIA) and GSE31210 datasets. Malignant tumor phenotypes were tested in vitro and in vivo through biological function assays and nude mouse with xenograft tumors. RNA immunoprecipitation assays were conducted to determine the interaction between METTL1 protein and FOXM1 mRNA. Public transcriptional database, Chromatin immunoprecipitation and luciferase report assays were conducted to detect the downstream target of a transcriptional factor FOXM1. Half maximal inhibitory concentration (IC50) was calculated to evaluate the sensitivity to Gefitinib in LUAD cells.ResultsThe results showed that METTL1 was upregulated in LUAD, and the high expression of METTL1 was associated with unfavorable prognosis. Through the m7G‐dependent manner, METTL1 improved the RNA stability of FOXM1, leading to the up‐regulation of FOXM1. FOXM1 transcriptionally suppressed PTPN13 expression. The METTL1/FOXM1/PTPN13 axis reduced the sensitivity of LUAD cells to Gefitinib. Taken together, our data suggested that METTL1 plays oncogenic role in LUAD through inducing the m7G modification of FOXM1, therefore METTL1 probably is a new potential therapeutic target to counteract Gefitinib resistance in LUAD.
Safety and efficacy of fruquintinib‐based therapy in patients with advanced or metastatic sarcoma
Authors: Zhou Chenliang , Qian Guowei , Wang Yonggang , Li Hongtao , Shen Zan , Zheng Shuier 
Published: July 2024

AbstractBackgroundThe purpose of this study was to evaluate the efficacy and safety of fruquintinib‐based therapy as a salvage therapy for patients with advanced or metastatic sarcoma, including soft tissue sarcoma (STS) and bone sarcoma.MethodsPatients with advanced or metastatic sarcoma were divided into two groups. One group received fruquintinib monotherapy, while the other received fruquintinib combined therapy. Safety and efficacy of fruquintinib‐based therapy were recorded and reviewed retrospectively, including progression‐free survival (PFS), overall response rate (ORR), and adverse events (AEs).ResultsBetween August 2021 and December 2022, 38 sarcoma patients were retrospectively included. A total of 14 patients received fruquintinib alone (including 6 STS and 8 bone sarcoma), while 24 were treated with fruquintinib combined therapy (including 2 STS and 22 bone sarcoma). The median follow‐up was 10.2 months (95% CI, 6.4–11.5). For the entire population, the median PFS was 8.0 months (95% CI, 5.5–13.0). The ORR was 13.1%, while the disease control rate (DCR) was 86.8%. The univariate analysis showed that radiotherapy history (HR, 4.56; 95% CI, 1.70–12.24; p = 0.003), bone sarcoma (HR, 0.34; 95% CI, 0.14–0.87; p = 0.024), and treatment method of fruquintinib (HR, 0.36; 95% CI, 0.15–0.85; p = 0.021) were significantly associated with PFS. The multivariate analysis showed that patients without radiotherapy history were associated with a better PFS (HR, 3.71; 95% CI: 1.31–10.55; p = 0.014) than patients with radiotherapy history. Patients in combination group reported pneumothorax (8.3%), leukopenia (33.3%), thrombocytopenia (12.5%), diarrhea (4.2%), and anemia (4.2%) as the most frequent grade 3 or higher treatment‐emergent AEs (TEAEs), while there was no severe TEAEs occurred in the monotherapy group.ConclusionsFruquintinib‐based therapy displayed an optimal tumor control and an acceptable safety profile in patients with advanced or metastatic sarcoma.
Pillar[5]arene‐Based Ion‐Pair Recognition for Encapsulation of a Stilbazolium‐Type Dye with Enhanced Photophysical Properties and Nonlinear Optical Activity
Authors: Li Ming , Yang Yuting , Liu Rui , Wang Yanfang , Shao Li , Hua Bin , Liu Xiaofeng , Huang Feihe 
Published: July 2024

AbstractConstructing organic composite materials through molecular recognition has emerged as an important theme in materials science. Here we report an ion‐pair recognition system involving the use of a propoxylated pillar[5]arene (PrP5) to modulate the solid‐state photophysical properties of dye trans‐4’‐(dimethylamino)‐N‐methyl‐4‐stilbazolium hexafluorophosphate (DMASP). Single crystal X‐ray diffraction analysis reveals that the dye guest DMASP is encapsulated by PrP5 to form a 2 : 1 host−guest complex 2PrP5⸧DMASP in the crystalline state. The macrocyclic skeleton of PrP5 imposes restrictions on the intramolecular motions of the dye guest, leading to a significant enhancement of its fluorescence emission. Additionally, within the 2PrP5⸧DMASP complex crystal structure, DMASP molecules are found to display two possible opposite orientations in the one‐dimensional channels formed by PrP5 molecules. This arrangement is believed to alter the overall solid‐state packing structure of DMASP, thereby activating its nonlinear optical activity. This work not only reports a novel ion‐pair molecular recognition system based on pillararenes but also provides valuable insights into the modulation of the crystalline state photophysical properties of organic dyes via cocrystal engineering.
Evaluation of secretome biomarkers in glioblastoma cancer stem cells: A bioinformatics analysis
Authors: Jangholi Ehsan , Tehran Hoda Ahmari , Ghasemi Afsaneh , Hoseinian Mohammad , Firoozi Sina , Ghodsi Seyed Mohammad , Tamaddon Mona , Bereimipour Ahmad , Hadjighassem Mahmoudreza 
Published: July 2024

AbstractBackgroundGlioblastoma (GBM) is a malignant brain tumor that frequently occurs alongside other central nervous system (CNS) conditions. The secretome of GBM cells contains a diverse array of proteins released into the extracellular space, influencing the tumor microenvironment. These proteins can serve as potential biomarkers for GBM due to their involvement in key biological processes, exploring the secretome biomarkers in GBM research represents a cutting‐edge strategy with significant potential for advancing diagnostic precision, treatment monitoring, and ultimately improving outcomes for patients with this challenging brain cancer.AimThis study was aimed to investigate the roles of secretome biomarkers and their pathwayes in GBM through bioinformatics analysis.Methods and ResultsUsing data from the Gene Expression Omnibus and the Cancer Genome Atlas datasets—where both healthy and cancerous samples were analyzed—we used a quantitative analytical framework to identify differentially expressed genes (DEGs) and cell signaling pathways that might be related to GBM. Then, we performed gene ontology studies and hub protein identifications to estimate the roles of these DEGs after finding disease‐gene connection networks and signaling pathways. Using the GEPIA Proportional Hazard Model and the Kaplan–Meier estimator, we widened our analysis to identify the important genes that may play a role in both progression and the survival of patients with GBM. In total, 890 DEGs, including 475 and 415 upregulated and downregulated were identified, respectively. Our results revealed that SQLE, DHCR7, delta‐1 phospholipase C (PLCD1), and MINPP1 genes are highly expressed, and the Enolase 2 (ENO2) and hexokinase‐1 (HK1) genes are low expressions.ConclusionHence, our findings suggest novel mechanisms that affect the occurrence of GBM development, growth, and/or establishment and may also serve as secretory biomarkers for GBM prognosis and possible targets for therapy. So, continued research in this field may uncover new avenues for therapeutic interventions and contribute to the ongoing efforts to combat GBM effectively.
Open and Closed Cage Silsesquioxane Dimers
Authors: Yonezawa Honoka , Naka Kensuke , Imoto Hiroaki 
Published: July 2024

AbstractPolyhedral oligomeric silsesquioxane (POSS) is an organic‐inorganic hybrid molecule with two structural variations, closed‐ and open‐cage configurations, referred to as completely condensed POSS (CC‐POSS) and corner‐opened POSS (CO‐POSS), respectively. In this study, we synthesized 12 dimers by combining CC‐ and CO‐POSS variants decorated with isobutyl or phenyl substituents to explore their structure‐property relationships. The choice of substituents, both at the cage vertices and open sites, significantly affected the thermal and optical properties of the materials. Modifying the substituents on CO‐ and CC‐POSS, which are isomers, led to significant alterations in the material properties. Notably, isomer‐bearing carbazole substituents exhibited a substantially higher quantum yield (0.32) than its counterpart isomer (0.13), underscoring the crucial role of structural nuances in determining material performance. These results offer valuable insights for the design of novel silsesquioxane‐based materials.
Ex Vivo Evaluation of the Function of Hematopoietic Stem Cells in Toxicology of Metals
Authors: Wu Jiaojiao , Liu Ting , Tang Mengke , Liu Yalin , Wang Wei , Wang Chuanxuan , Ju Yingzi , Zhao Yifan , Zhang Yubin 
Published: July 2024

AbstractA variety of metals, e.g., lead (Pb), cadmium (Cd), and lithium (Li), are in the environment and are toxic to humans. Hematopoietic stem cells (HSCs) reside at the apex of hematopoiesis and are capable of generating all kinds of blood cells and self‐renew to maintain the HSC pool. HSCs are sensitive to environmental stimuli. Metals may influence the function of HSCs by directly acting on HSCs or indirectly by affecting the surrounding microenvironment for HSCs in the bone marrow (BM) or niche, including cellular and extracellular components. Investigating the impact of direct and/or indirect actions of metals on HSCs contributes to the understanding of immunological and hematopoietic toxicology of metals. Treatment of HSCs with metals ex vivo, and the ensuing HSC transplantation assays, are useful for evaluating the impacts of the direct actions of metals on the function of HSCs. Investigating the mechanisms involved, given the rarity of HSCs, methods that require large numbers of cells are not suitable for signal screening; however, flow cytometry is a useful tool for signal screening HSCs. After targeting signaling pathways, interventions ex vivo and HSCs transplantation are required to confirm the roles of the signaling pathways in regulating the function of HSCs exposed to metals. Here, we describe protocols to evaluate the mechanisms of direct and indirect action of metals on HSCs. © 2024 Wiley Periodicals LLC.This article was corrected on 18 July 2024. See the end of the full text for details.Basic Protocol 1: Identify the impact of a metal on the competence of HSCsBasic Protocol 2: Identify the impact of a metal on the lineage bias of HSC differentiationBasic Protocol 3: Screen the potential signaling molecules in HSCs during metal exposureAlternate Protocol 1: Ex vivo treatment with a metal on purified HSCsAlternate Protocol 2: Ex vivo intervention of the signaling pathway regulating the function of HSCs during metal exposure
Orsay Virus Infection in Caenorhabditis elegans
Authors: Batachari Lakshmi E. , Sarmiento Mario Bardan , Wernet Nicole , Troemel Emily R. 
Published: July 2024

AbstractOrsay virus infection in the nematode Caenorhabditis elegans presents an opportunity to study host‐virus interactions in an easily culturable, whole‐animal host. Previously, a major limitation of C. elegans as a model for studying antiviral immunity was the lack of viruses known to naturally infect the worm. With the 2011 discovery of the Orsay virus, a naturally occurring viral pathogen, C. elegans has emerged as a compelling model for research on antiviral defense. From the perspective of the host, the genetic tractability of C. elegans enables mechanistic studies of antiviral immunity while the transparency of this animal allows for the observation of subcellular processes in vivo. Preparing infective virus filtrate and performing infections can be achieved with relative ease in a laboratory setting. Moreover, several tools are available to measure the outcome of infection. Here, we describe workflows for generating infective virus filtrate, achieving reproducible infection of C. elegans, and assessing the outcome of viral infection using molecular biology approaches and immunofluorescence. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Preparation of Orsay virus filtrateSupport Protocol: Synchronize C. elegans development by bleachingBasic Protocol 2: Orsay virus infectionBasic Protocol 3: Quantification of Orsay virus RNA1/RNA2 transcript levels by qRT‐PCRBasic Protocol 4: Quantification of infection rate and fluorescence in situ hybridization (FISH) fluorescence intensityBasic Protocol 5: Immunofluorescent labeling of dsRNA in virus‐infected intestinal tissue
Pecan Aphid Complex and Their Management
Authors: Barman Apurba , Acharya Rajendra , Veerlapati Shivakumar 
Published: July 2024

AbstractPecan production in the U.S. is primarily divided into two production regions: the southwestern region and the southeastern region. One insect pest complex is common to both production regions—pecan aphids. Growers regularly make insecticide applications to manage these aphids every year. The pecan aphid complex is represented by three aphid species: yellow pecan aphid, the blackmargined aphid, and the black pecan aphid. This article will discuss some of the important aspects of pecan aphids in relation to their management in commercial pecan orchards. Earn 0.5 CEUs in Integrated Pest Management by reading this article and taking the quiz at https://web.sciencesocieties.org/Learning‐Center/Courses.