bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Reinforcement Effect of High Pressure Jet Grouting Pile During Water Inflow of Loess Tunnel Basement During Operation Period
Authors: Yang Zhanhu , Dai Jinpeng , Wang Qicai , Tian Xincheng , Liu Hao 
Published: July 2024

AbstractIn the built loess tunnel, a large number of diseases have been found during the operation period, especially when the tunnel basement is after water inflow. At present, a large number of high pressure jet grouting pile are used to reinforce the tunnel basement, but there are few studies on the reinforcement effect of high pressure jet grouting pile when the tunnel basement is after water inflow during the operation period. Due to the operation control of the expressway, it is difficult to carry out field research during the operation period. In this paper, by changing the physical parameters of the water inflow layer of the surrounding rock of the tunnel basement, numerical simulation is used to analyze the reinforcement effect of high pressure jet grouting pile on the tunnel basement under the loads, and field monitoring means are used to confirm the correctness of the three-dimensional model. The results show that the reinforcement of high pressure jet grouting pile can prevent the uplift caused by the softening of tunnel basement surrounding rock. The error of monitoring data and simulation results is also within a reasonable range.
Analysis of Tunnel Excavation Deformation Based on FLAC3D
Authors: Ge Wensheng 
Published: July 2024

AbstractDeformation analysis of tunnel excavation is one of the core problems. FLAC3D is used to simulate tunnel excavation, including tunnel modeling, excavation and support, and analyzes the force and deformation characteristics in the construction process. On the basis of collecting tunnel data, made reasonable monitoring measurement scheme, and measurement of the real-time monitoring data for the initial analysis, analysis the excavation time and excavation length regarding the deformation of the surrounding rock, predicted the deformation trend, determine the total displacement of the surrounding rock deformation, and map the relationship to time to provide a reasonable time for the secondary lining, using FLAC3D software for numerical simulation of tunnel excavation, including tunnel modeling, excavation, support, and analyzed the force and deformation characteristics in the construction process. Different combined values of K and E are designed and applying the model built by the software as the normal calculation program of numerical simulation to obtain different displacement values of arch subsidence and hole circumference convergence.
Calculation of Active Earth Pressure on a Circular Retaining Wall Based on Energy Method
Authors: Jia Senlin , Zhou Guigui , Qin Tao , Mei Yifan , Li Jiahui , Lu Kunlin 
Published: July 2024

AbstractFor an axisymmetric circular retaining wall, a calculation method of active earth pressure on the circular retaining wall based on energy method is being proposed. The analysis of the soil behind the wall employs the Coulomb failure mechanism. Assuming the soil to be a completely rigid plastic body, the calculation formula of active earth pressure on the circular retaining wall is being established according to the associated flow law and virtual work principle. The results are indicating that the active earth pressure on the circular retaining wall is lower than that predicted by the Coulomb solution for plane retaining walls. This active earth pressure is increasing as the radius-to-height ratio of the wall increases, eventually aligning with the Coulomb solution for plane walls. Additionally, the inclination angle of the slip surface is being observed to increase with the circumferential stress coefficient, while the active earth pressure is decreasing correspondingly; For non-cohesive soil, when the radius-to-height ratio approaches infinity, the calculation method in this paper is being consistent with Coulomb theory. This method can calculate the active earth pressure on circular retaining wall with cohesive soil behind the wall. As the cohesion increases, the inclination angle of the slip surface rises, and the active earth pressure decreases. The result is being confirmed by model tests. It can provide a reference for the engineering design of circular retaining structures.
A novel Bacillus aerolatus CX253 attenuates inflammation induced by Streptococcus pneumoniae in childhood and pregnant rats by regulating gut microbiome
Authors: Yu Ting , Wu Biru , Zhang Dimei , Deng Guanhua , Luo Yi , Tang Ningqianzi , Shi Qiankun , Hu Fang , Zhang Guoxia 
Published: July 2024

AbstractStreptococcus pneumoniae (Spn) is the predominant pathogen responsible for community-acquired pneumonia (CAP) in children under five years old, and it can induce over 17% of pregnant women. However, no more effective measures exist to prevent infection induced by Spn in these two special populations. The beneficial microbes can antagonize Spn and provide new targets for preventing pneumococcal infections. This study used 16S rRNA gene sequencing and targeted metabolomics to evaluate the role of the Bacillus aerolatus CX253 (CX253) in alleviating Spn infection. Additionally, the colonization of CX253 was observed in nose, trachea, and lung by using confocal laser scanning microscopy and fluorescent labeling techniques. Compared with the model group, the expression level of interleukin-1β was dropped 1.81-fold and 2.22-fold, and interleukin-6 was decreased 2.39-fold and 1.84-fold. The express of tumor necrosis factor-α was down 2.30-fold and 3.84-fold in prevention group of childhood and pregnant rats, respectively. The 16S rRNA sequencing results showed that CX253 administration alone significantly increased the abundance of Lactobacillus, Limosilactobacillus, and Prevotella in the gut of childhood and pregnant rats. Furthermore, the CX253 increased propionate in the gut of childhood rats and increased propionate and butyrate in the gut of pregnant rats to inhibit pulmonary inflammation. In summary, CX253 attenuated Spn-induced inflammation by regulating the gut microbiota and SCFAs. The research provides valuable information for the prevention of pneumonia.
Graphical Abstract
The cellular adaptor GULP1 interacts with ATG14 to potentiate autophagy and APP processing
Authors: Chau Dennis Dik-Long , Yu Zhicheng , Chan Wai Wa Ray , Yuqi Zhai , Chang Raymond Chuen Chung , Ngo Jacky Chi Ki , Chan Ho Yin Edwin , Lau Kwok-Fai 
Published: July 2024

AbstractAutophagy is a highly conserved catabolic mechanism by which unnecessary or dysfunctional cellular components are removed. The dysregulation of autophagy has been implicated in various neurodegenerative diseases, including Alzheimer’s disease (AD). Understanding the molecular mechanism(s)/molecules that influence autophagy may provide important insights into developing therapeutic strategies against AD and other neurodegenerative disorders. Engulfment adaptor phosphotyrosine-binding domain-containing protein 1 (GULP1) is an adaptor that interacts with amyloid precursor protein (APP) to promote amyloid-β peptide production via an unidentified mechanism. Emerging evidence suggests that GULP1 has a role in autophagy. Here, we show that GULP1 is involved in autophagy through an interaction with autophagy-related 14 (ATG14), which is a regulator of autophagosome formation. GULP1 potentiated the stimulatory effect of ATG14 on autophagy by modulating class III phosphatidylinositol 3-kinase complex 1 (PI3KC3-C1) activity. The effect of GULP1 is attenuated by a GULP1 mutation (GULP1m) that disrupts the GULP1–ATG14 interaction. Conversely, PI3KC3-C1 activity is enhanced in cells expressing APP but not in those expressing an APP mutant that does not bind GULP1, which suggests a role of GULP1–APP in regulating PI3KC3-C1 activity. Notably, GULP1 facilitates the targeting of ATG14 to the endoplasmic reticulum (ER). Moreover, the levels of both ATG14 and APP are elevated in the autophagic vacuoles (AVs) of cells expressing GULP1, but not in those expressing GULP1m. APP processing is markedly enhanced in cells co-expressing GULP1 and ATG14. Hence, GULP1 alters APP processing by promoting the entry of APP into AVs. In summary, we unveil a novel role of GULP1 in enhancing the targeting of ATG14 to the ER to stimulate autophagy and, consequently, APP processing.
Pharmacological induction of the hypoxia response pathway in Huh7 hepatoma cells limits proliferation but increases resilience under metabolic stress
Authors: Jacquemin Clémence , El Orch Walid , Diaz Olivier , Lalande Alexandre , Aublin-Gex Anne , Jacolin Florentine , Toesca Johan , Si-Tahar Mustapha , Mathieu Cyrille , Lotteau Vincent , Perrin-Cocon Laure , Vidalain Pierre-Olivier 
Published: July 2024

AbstractThe hypoxia response pathway enables adaptation to oxygen deprivation. It is mediated by hypoxia-inducible factors (HIF), which promote metabolic reprogramming, erythropoiesis, angiogenesis and tissue remodeling. This led to the successful development of HIF-inducing drugs for treating anemia and some of these molecules are now in clinic. However, elevated levels of HIFs are frequently associated with tumor growth, poor prognosis, and drug resistance in various cancers, including hepatocellular carcinoma (HCC). Consequently, there are concerns regarding the recommendation of HIF-inducing drugs in certain clinical situations. Here, we analyzed the effects of two HIF-inducing drugs, Molidustat and Roxadustat, in the well-characterized HCC cell line Huh7. These drugs increased HIF-1α and HIF-2α protein levels which both participate in inducing hypoxia response genes such as BNIP3, SERPINE1, LDHA or EPO. Combined transcriptomics, proteomics and metabolomics showed that Molidustat increased the expression of glycolytic enzymes, while the mitochondrial network was fragmented and cellular respiration decreased. This metabolic remodeling was associated with a reduced proliferation and a lower demand for pyrimidine supply, but an increased ability of cells to convert pyruvate to lactate. This was accompanied by a higher resistance to the inhibition of mitochondrial respiration by antimycin A, a phenotype confirmed in Roxadustat-treated Huh7 cells and Molidustat-treated hepatoblastoma cells (Huh6 and HepG2). Overall, this study shows that HIF-inducing drugs increase the metabolic resilience of liver cancer cells to metabolic stressors, arguing for careful monitoring of patients treated with HIF-inducing drugs, especially when they are at risk of liver cancer.
The impact of hypoglycaemia on daily functioning among adults with diabetes: a prospective observational study using the Hypo-METRICS app
Authors: Søholm Uffe , Broadley Melanie , Zaremba Natalie , Divilly Patrick , Baumann Petra Martina , Mahmoudi Zeinab , Martine-Edith Gilberte , Mader Julia K. , Cigler Monika , Brøsen Julie Maria Bøggild , Vaag Allan , Heller Simon , Pedersen-Bjergaard Ulrik , McCrimmon Rory J. , Renard Eric , Evans Mark , de Galan Bastiaan , Abbink Evertine , Amiel Stephanie A. , Hendrieckx Christel , Speight Jane , Choudhary Pratik , Pouwer Frans ,   on behalf of the Hypo-RESOLVE consortium
Published: July 2024

Abstract
Aims/hypothesis
The aim of this work was to examine the impact of hypoglycaemia on daily functioning among adults with type 1 diabetes or insulin-treated type 2 diabetes, using the novel Hypo-METRICS app.

Methods
For 70 consecutive days, 594 adults (type 1 diabetes, n=274; type 2 diabetes, n=320) completed brief morning and evening Hypo-METRICS ‘check-ins’ about their experienced hypoglycaemia and daily functioning. Participants wore a blinded glucose sensor (i.e. data unavailable to the participants) for the study duration. Days and nights with or without person-reported hypoglycaemia (PRH) and/or sensor-detected hypoglycaemia (SDH) were compared using multilevel regression models.

Results
Participants submitted a mean ± SD of 86.3±12.5% morning and 90.8±10.7% evening check-ins. For both types of diabetes, SDH alone had no significant associations with the changes in daily functioning scores. However, daytime and night-time PRH (with or without SDH) were significantly associated with worsening of energy levels, mood, cognitive functioning, negative affect and fear of hypoglycaemia later that day or while asleep. In addition, night-time PRH (with or without SDH) was significantly associated with worsening of sleep quality (type 1 and type 2 diabetes) and memory (type 2 diabetes). Further, daytime PRH (with or without SDH), was associated with worsening of fear of hyperglycaemia while asleep (type 1 diabetes), memory (type 1 and type 2 diabetes) and social functioning (type 2 diabetes).

Conclusions/interpretation
This prospective, real-world study reveals impact on several domains of daily functioning following PRH but not following SDH alone. These data suggest that the observed negative impact is mainly driven by subjective awareness of hypoglycaemia (i.e. PRH), through either symptoms or sensor alerts/readings and/or the need to take action to prevent or treat episodes.

Graphical Abstract
The full spectrum of clinical stages of psychosis at prison entry: prevalence and concurrent validity of symptom screening
Authors: Yee Natalia , Browne Christie , Chemjong Prabin , Korobanova Daria , Dean Kimberlie 
Published: July 2024

Abstract

Purpose
Despite the high rates of psychotic disorders amongst people in prison, current prison mental health screening approaches have not included assessment of the full psychosis spectrum to capture those at-risk of an emerging psychosis as well as those with established illness nor assessed the concurrent validity of psychosis symptom screening.

Methods
Using a clinical staging approach to establish the prevalence of Ultra High Risk (UHR), first episode of psychosis (FEP) and established psychosis (EP) groups, 291 adults entering custody in two prison reception centres in NSW completed a two-stage (screening and validation) interview process. The Comprehensive Assessment of At-Risk Mental States (CAARMS) was used to determine the clinical stages of psychosis and concurrent validity of symptom screening in identifying individuals on the psychosis spectrum was formally assessed.

Results
Amongst men and women entering prison, almost one quarter (24.1%) met UHR criteria, 5.1% met the FEP threshold and 10.6% had an established psychosis. Those on the psychosis spectrum reported greater disadvantage across sociodemographic and justice factors. The presence of perceptual disturbance and paranoid beliefs emerged as the two best screening items for identifying those with an underlying psychosis spectrum illness.

Conclusion
The prevalence of psychosis spectrum illness, including the UHR state, amongst those entering prison is high. Current prison mental health approaches should include screening for the presence of perceptual disturbances and paranoid beliefs to improve the detection of psychosis spectrum illness.
Tech hubs within cities—determinants and dynamics
Authors: Hellwig Vanessa 
Published: July 2024

AbstractThis paper explores the microgeographic determinants of firm birth activity and relocation patterns in the digital economy simultaneously. The study investigates the spatial dynamics of young firms within Berlin, Hamburg, and Munich using geo-coded data of firm births and relocation from 2009 to 2016 aggregated at a 1 × 1 km2 grid level. The panel fixed effect regression models examine the impact of changes within persistent city structures on the location choices using several control variables. The findings suggest that firm clustering, commercial building density, and higher rent prices positively influence firm births, while the impact of knowledge institutions in the neighborhood is mixed. Relocation patterns highlight the role of proximity to competitors, with higher prices in areas not deterring relocations. These findings contribute to understanding firm location choices within cities by considering the two avenues of growth and highlight the future need for further investigations on the microgeography of firm growth dynamics.
Thermal imaging from UAS for estimating crop water status in a Merlot vineyard in semi-arid conditions
Authors: Atencia Payares Luz K. , Gomez-del-Campo Maria , Tarquis Ana M. , García Mónica 
Published: July 2024

Abstract
Thermal remote sensing indicators of crop water status can help to optimize irrigation across time and space. The Crop Water Stress Index (CWSI), calculated from thermal data, has been widely used in irrigation management as it has a proven association with evapotranspiration ratios. However, different approaches can be used to calculate the CWSI. The aim of this study is to identify the most robust method for estimating the CWSI in a commercial Merlot vineyard using high-resolution thermal imaging from Unoccupied Aerial Systems (UAS). To that end, three different methods were used to estimate the CWSI: Jackson’s model (CWSIj), Wet Artificial Reference Surface (WARS) method (CWSIw), and the Bellvert approach (CWSIb). A simpler indicator calculated as the difference between canopy and air temperature (Tc–Ta) was the benchmark to beat. The water status of a vine cultivar with anisohydric behavior (Merlot) in a vineyard in central Spain was assessed for two years with different agroclimatic conditions. Canopy temperature (Tc) was obtained from UAS flights at 9:00 h and 12:00 h solar hour over eight days during the irrigation period (June–August), and from vines under five different irrigation treatments. Stem water potential (SWP), stomatal conductance (gs), and leaf temperature (TL) were recorded at the time of the flights and compared with the thermal indices (CWSIj, CWSIw, CWSIb) and the benchmark indicator (Tc–Ta). Results show that the simpler indicator of water stress, Tc–Ta, performed better at identifying varying levels of crop hydration than CWSIb or CWSIw at 12:00 h. Under conditions of extreme aridity, the latter indices were less accurate than the physically-based CWSIj at 12:00 h, which had the highest correlation with SWP (r = 0.84), followed by the benchmark index Tc–Ta (r = 0.70 at 12:00). Considering the current climatic trends towards aridification, the CWSIj emerges as a useful operational tool, with robust performance across days and times of day. These results are important for irrigation management and could contribute to improving water use efficiency in agriculture.
Biochemical analysis of catecholamine and cortisol for the evaluation of the fetal distress in third trimester stillbirths
Authors: Giorgetti Arianna , Bonasoni Maria Paola , Lacchè Elena , Comitini Giuseppina , Migliavacca Costanza , Ferretti Alice , Galeone Carla , Polese Alessandra , Stridi Giovanna , Monari Francesca , Melis Beatrice , Pelotti Susi 
Published: July 2024

Abstract
Background
Stress hormones like catecholamine and cortisol are thought to reflect the magnitude of physical stress in adults and were studied in relationship to the cause of death and agony time. Intrauterine distress, intrapartum events, and modes of delivery can affect the fetal endocrine stress response, as reflected by biochemical analyses. The aim of the present study was to evaluate the role of catecholamines and cortisol as markers of ante-mortem fetal distress. The role of cortisol as a marker of circadian timing of delivery was also assessed.

Methods
A 2-year prospective cohort-comparison inclusion of stillbirths and newborns took place with collection of antemortem data, labor parameters, neonatal outcome, post-mortem data and blood samples. Stillbirths were classified as acute or chronic on the basis of a multidisciplinary evaluation. Heart blood of stillbirths and cord blood of newborns were analyzed by high pressure liquid chromatography (HPLC) for adrenaline and noradrenaline and by immunoassay for cortisol determination.

Results
Fifteen stillbirths and 46 newborns, as a comparison group, delivered by spontaneous vaginal birth, elective, and emergency cesarean sections were included. Stillbirths’ main cause of death was cord thrombosis. Levels of adrenaline and noradrenaline (median: 14,188 pg/ml and 230.5 pg/ml, respectively) were significantly higher (p < 0.001) in stillbirths than in newborns and were also higher in acute compared to chronic distress. Cortisol levels were significantly higher (p < 0.05) in spontaneous vaginal delivery (median: 18.2 μg/dl) compared to elective cesarean sections (median: 3.8 μg/dl). No difference in cortisol concentrations was detected between newborns delivered at morning and at afternoon/evening.

Conclusion
Our results suggest that the biochemical measurement of adrenaline and noradrenaline levels might reflect a marked physical stress response during the process of death in stillbirths. On the contrary, the elevation of cortisol levels could mirror the elevation in maternal cortisol level during vaginal delivery. For the post-mortem evaluation of stillbirths, the analysis of CA levels could provide additional data on the duration of distress, useful to integrate the forensic diagnosis.
Morphological and immunohistochemical evaluation in distinguishing post-radiotherapy serous-like endometrial change (PoRSEC) and serous endometrial intraepithelial carcinoma (SEIC)
Authors: Arciuolo Damiano , Scaglione Giulia , Travaglino Antonio , D’Alessandris Nicoletta , Santoro Angela , Inzani Frediano , Urtueta Belen Padial , Sfregola Stefania , Raffone Antonio , Fulgione Caterina , Valente Michele , Benvenuto Roberta , Cianfrini Federica , Zannoni Gian Franco 
Published: July 2024

AbstractUteri from women undergoing chemoradiotherapy (CRT) may show reactive atypia which may mimic serous endometrial intraepithelial carcinoma (SEIC). We aimed to assess the prevalence and morphological/immunohistochemical features of post-radiotherapy serous-like endometrial changes (PoRSEC) in women undergone CRT for locally advanced cervical cancer, with a focus on the differential diagnosis with SEIC. Consecutive patients with locally advanced cervical cancer undergone CRT between 2011 and 2018 were reviewed. Endometrial histological specimens were assessed for the presence of PoRSEC. Twenty-two cases of SEIC were included for comparison. Immunohistochemistry for p53, p16, and Ki67 was performed. Out of 244 reviewed patients, 36 (14.7%) showed PoRSEC. The degree of nuclear atypia was similar between PoRSECs and SEIC. However, a papillary architecture with areas of confluent papillae was only observed in SEIC. SEIC cases showed a high mitotic activity as opposed to PoRSEC cases. The expression of p53 was aberrant in all SEICs but in none of the PoRSECs; however, 13/36 PoRSECs showed p53 positivity in most tumor cells, potentially mimicking a mutation pattern. A block-type p16 expression was observed in all SEICs and in 16/36 PoRSECs. Mean Ki67 expression was 26.9% in SEIC (range 5–70%) and 8.16% in PoRSEC (range 5–35%). While SEIC showed sharp morphological and immunohistochemical demarcation, PoRSEC were more heterogenous and merged imperceptibly with normal endometrium. In conclusion, PoRSEC may mimic SEIC both morphologically and immunohistochemically. However, a papillary architecture with cytological demarcation is typically observed in SEIC but not in PoRSEC.