bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cinnamaldehyde and Nitric Oxide Attenuate Advanced Glycation End Products‐Induced the JAK/STAT Signaling in Human Renal Tubular Cells
Authors: Huang Jau‐Shyang , Lee Ying‐Ho , Chuang Lea‐Yea , Guh Jinn‐Yuh , Hwang Jean‐Yu 
Published: January 2015

ABSTRACTCinnamaldehyde is a major and a bioactive compound isolated from the leaves of Cinnamomum osmophloeum kaneh. It possesses anti‐diabetic properties in vitro and in vivo and has anti‐inflammatory and anti‐cancer effects. To explore whether cinnamaldehyde was linked to altered advanced glycation end products (AGE)‐mediated diabetic nephropathy, the molecular mechanisms of cinnamaldehyde responsible for inhibition of AGE‐reduced nitric oxide (NO) bioactivity in human renal proximal tubular cells were examined. We found that raising the ambient AGE concentration causes a dose‐dependent decrease in NO generation. Cinnamaldehyde significantly reverses AGE‐inhibited NO generation and induces high levels of cGMP synthesis and PKG activation. Treatments with cinnamaldehyde, the NO donor S‐nitroso‐N‐acetylpenicillamine, and the JAK2 inhibitor AG490 markedly attenuated AGE‐inhibited NOS protein levels and NO generation. Moreover, AGE‐induced the JAK2‐STAT1/STAT3 activation, RAGE/p27Kip1/collagen IV protein levels, and cellular hypertrophy were reversed by cinnamaldehyde. The ability of cinnamaldehyde to suppress STAT activation was also verified by the observation that it significantly increased SCOS‐3 protein level. These findings indicate for the first time that in the presence of cinnamaldehyde, the suppression of AGE‐induced biological responses is probably mediated by inactivating the JAK2‐STAT1/STAT3 cascade or activating the NO pathway. J. Cell. Biochem. 116: 1028–1038, 2015. © 2015 Wiley Periodicals, Inc.
MiR‐210 Links Hypoxia With Cell Proliferation Regulation in Human Laryngocarcinoma Cancer
Authors: Zuo Jianhong , Wen Meiling , Lei Mingsheng , Peng Xiang , Yang Xuefeng , Liu Zhigang 
Published: January 2015

ABSTRACTThe microRNA hsa‐miR‐210 (miR‐210) is associated with hypoxia; however its function has not fully identified. In the present study, we aim to detect its role concerning proliferation in Laryngocarcinoma. We found that miR‐210 was highly expressed in hypoxia, which inhibited proliferation by inducing cell cycle arrest in G1/G0 as well as apoptosis. We further identified that miR‐210 targeted fibroblast growth factor receptor‐like 1 (FGFRL1). Down regulation of FGFRL1 decreased cell proliferation by promoting proportion of cells in G1/G0 phase and decreasing in S and G2/M phases. Moreover, overexpression of FGFRL1 effectively released the miR‐210‐induced suppression of SCC10A cell proliferation. Expression of miR‐210 repressed tumor xenograft growth in vivo as well. Together, our findings reveal a new mechanism of adaptation to hypoxia that miR‐210 inhibits the proliferation via inducing cell cycle arrest and apoptosis by the targeting of FGFRL1. J. Cell. Biochem. 116: 1039–1049, 2015. © 2015 Wiley Periodicals, Inc.
Retracted: MiR‐17–5p Up‐Regulates YES1 to Modulate the Cell Cycle Progression and Apoptosis in Ovarian Cancer Cell Lines
Authors: Li Lan , He Li , Zhao Jian‐Li , Xiao Jing , Liu Min , Li Xin , Tang Hua 
Published: January 2015

ABSTRACTMicroRNAs (miRNAs) are small, non‐coding RNAs that participate in the regulation of gene expression. Although many studies have demonstrated the involvement of miR‐17–5p in different cancers, little is known to its function in ovarian cancer. In this study, we demonstrated that overexpression of miR‐17–5p was able to enhance cell proliferation by promoting G1/S transition of the cell cycle and suppressing apoptosis in ES‐2 and OVCAR3 cell lines, whereas inhibition of miR‐17–5p yielded the reverse phenotype. YES1 was identified as a novel target gene of miR‐17–5p. Moreover, miR‐17–5p was found to directly bind to the 3′UTR of YES1 mRNA and up‐regulated its expression. Furthermore, knockdown of YES1 led to the suppression of proliferation and induced cell cycle arrest in ES‐2 and OVCAR3 cells. Ectopic expression of YES1 was able to reverse the effects of miR‐17–5p inhibition. Collectively, our results indicated that miR‐17–5p might play a role in human ovarian cancer by up‐regulating YES1 expression. J. Cell. Biochem. 116: 1050–1059, 2015. © 2015 Wiley Periodicals, Inc.
Caveolae May Enable Albumin to Enter Human Renal Glomerular Endothelial Cells
Authors: Moriyama Takahito , Takei Takashi , Itabashi Mitsuyo , Uchida Keiko , Tsuchiya Ken , Nitta Kosaku 
Published: January 2015

ABSTRACTCaveolae on human renal glomerular endothelial cells (HRGECs) are increased in glomerular disease and correlate with the degree of albuminuria. To assess the mechanism by which caveolae contribute to albuminuria, we investigated whether albumin enters into HRGECs through caveolae. HRGECs were incubated with Alexa Fluor 488 labeled BSA or transferrin, followed by immunofluorescence localization with antibody to caveolin‐1 (Cav‐1), the main structural protein of caveolae, or clathrin, the major structural protein of clathrin coated pits, to assess whether BSA colocalized with Cav‐1. HRGECs were also incubated with albumin and caveolae disrupting agents, including methyl beta cyclodextrin (MBCD) and nystatin, to determine whether disrupting caveolae interfered with albumin endocytosis into HRGECs. HRGECs were also incubated with albumin after transfection with Cav‐1 small interfering RNAs (siRNAs). Labeled BSA colocalized with Cav‐1, but not with clathrin. In contrast, labeled transferrin colocalized with clathrin, but not with Cav‐1. Incubation of HRGECs with MBCD or nystatin, or transfection with Cav‐1 siRNA, significantly reduced the intracellular amounts of albumin and Cav‐1, relative to normal HRGECs, as shown by western blotting and immunofluorescence. These findings indicate that albumin enters HRGECs through the caveolae, suggesting that caveolae play an important role in the pathogenesis of albuminuria by providing a pathway through which albumin can enter glomerular endothelial cells. J. Cell. Biochem. 116: 1060–1069, 2015. © 2015 Wiley Periodicals, Inc.
Inactivation of the Phosphatidylinositol 3‐Kinase/Akt Pathway is Involved in BMP9‐mediated Tumor‐suppressive Effects in Gastric Cancer Cells
Authors: Duan Liang , Ye Liwei , Wu Rui , Wang Haiyan , Li Xueru , Li Huan , Yuan Shimei , Zha He , Sun Hui , Zhang Yunyuan , Chen Xian , Zhang Yan , Zhou Lan 
Published: January 2015

ABSTRACTBone morphogenetic proteins (BMPs) are members of the TGF‐β superfamily signaling factors. Expression of several BMPs (BMP2, BMP4, and BMP7) is correlated to poor prognosis in gastric cancer patients. The function of BMP9, the latest discovered and most powerful osteogenetic factor, in gastric cancer is relatively unclear. In this report, we investigated the expression, function and underlying molecular mechanisms of BMP9 in gastric cancer. The results show that BMP9 expression was markedly decreased in gastric cancer tissues and cell lines. Enforced BMP9 expression in the gastric cancer cell lines SGC‐7901 and MNK‐45 increased apoptosis and reduced viability and migration. The in vivo function of BMP9 was evaluated in a xenograft mouse model. Tumors derived from SGC‐7901 cells with enforced BMP9 expression (SGC‐7901/BMP9) showed significantly reduced size and weight compared to that from control cells. Enforced BMP9 expression resulted in decreased Akt activity shown as lower levels of phosphorylation at Ser473 and Thr308 in Akt. The PI3K/Akt inhibitor LY294002 potentiated BMP9's viability and migration suppression, and apoptosis induction, which was associated with reduced expression of snail and VEGF and increased expression of E‐cadherin. In addition, tumors derived from SGC‐7901/BMP9 showed reduced Akt activity and VEGF expression, and increased E‐cadherin expression. Therefore, our studies reveal for the first time that inhibition of the PI3K‐Akt pathway is involved in the tumor suppressor effects of BMP9 in gastric cancer. J. Cell. Biochem. 116: 1080–1089, 2015. © 2015 Wiley Periodicals, Inc.
Regulatory review time for approval of oncology drugs in Japan between 2001 and 2014. Considerations of changes, factors that affect review time, and difference with the United States
Authors: Maeda Hideki , Kurokawa Tatsuo 
Published: January 2015

AbstractIn this study, we comprehensively and historically studied the review time of oncology drugs approved by the regulatory authorities in Japan with publicly available information. A total of 120 applications of oncology drugs were approved in Japan between April 2001 and July 2014. The review time peaked with 732.0 days (24.4 months) in 2005, and showed a tendency to decline gradually each year thereafter. After 2012, a significant reduction of the review time was seen in comparison to the median of 13‐year median time. In addition, we compared the review time with that in the United States. The median review time lag with the United States was significantly peaked in 2005. After 2005, the review time lag with the FDA has decreased, but lag did not significantly reduce by 2014. We also examined factors influencing the review time in Japan with multiple regression analysis. It was found that the factors related to a use of overseas data and expedited program for accelerating the reviews influenced the direction of shortening the review time. We consider that regulatory authorities in Japan need to keep making efforts to reduce the review time further and eliminate the review time lag with the United States.
Gastrointestinal stromal tumors (GISTs) at uncommon locations: A large population based analysis
Authors: Kukar Moshim , Kapil Aditi , Papenfuss Wesley , Groman Adrienne , Grobmyer Stephen R. , Hochwald Steven N. 
Published: January 2015

IntroductionSparse information is available about GISTs in uncommon locations. Our large database analysis aims to determine the characteristics of GISTs in the esophagus, colon and rectum and compare to gastric GISTs.MethodsThe Surveillance Epidemiology and End Results (SEER) database was queried from 1990 to 2009 using CS SCHEMA v0203. Characteristics of each location were compared to gastric GISTs.Results4411 GIST (29 esophageal, 2658 stomach, 1463 small intestine, 126 colonic, and 135 rectal) from 1990 to 2009 were identified. Univariate and multivariate predictors of worse disease specific survival in both the entire cohort and surgical resection group include older age, male gender, tumor size > 5cm, no surgical intervention and anatomical location. Although less likely to undergo surgical resection, esophageal GIST (all patients and resected) had a comparable survival to gastric GIST. A higher proportion of colonic GISTs presented with distant disease and had a worse disease specific survival when compared to rectal GISTs.ConclusionOur results show a rising incidence in GISTs and highlight the characteristics of GISTs based on anatomical location. In addition, this is the first study to demonstrate that colonic GISTs behave differently when compared to rectal GISTs and warrants further prospective evaluation. J. Surg. Oncol. 2015 111:696–701. © 2015 Wiley Periodicals, Inc.
Quantitative assessment of the effect of cholesterol on blood glucose measurement using near infrared spectroscopy and a method for error reduction
Authors: Jiang Jingying , Zhang Kai , Qin Jia , Min Xiaolin , Zhang Lingling , Zou Da , Xu Kexin 
Published: January 2015

Background and ObjectiveThere is a growing body of evidence suggesting that the accurate measurement of blood glucose concentration can be perturbed by many factors. Current literature is limited in describing the influence of cholesterol on non‐invasive blood glucose measurements by near‐infrared spectroscopy (NIRS). This study aims to investigate the influence of cholesterol on blood glucose measurement through clinical oral glucose tolerance test (OGTT) and NIRS. Further, a method to reduce the prediction errors induced by cholesterol is proposed, facilitating the clinical application of non‐invasive blood glucose sensing by NIRS.Study Design/Material and MethodsWe obtained clinical data of glucose and cholesterol concentrations at specific time points (0, 0.5, 1, 2, and 3 h) during OGTTs from 115 subjects. The subjects were grouped into: Norm for normal control, IGT for Impaired Glucose Tolerance, and Diabetes. In addition, spectral data between 1200 and 1800 nm were collected from 130 phantom samples, which are separated into seven groups depending on glucose and cholesterol levels. Statistical methods including One Sample T‐test (OSTT), Pearson Correlation Analysis(PCA), and Unary Linear Regression (ULR) were used to analyze clinical data and spectral data to determine the relationship between glucose and cholesterol concentrations with the time course of OGTT. Reference wavelength‐based method (RWM) was introduced to diminish the influence of cholesterol on glucose measurement and further the prediction error induced by cholesterol was reduced when using partial least square (PLS) model.ResultsClinical results statistically show that there is a strong negative correlation between the changes of glucose and cholesterol concentrations in the diabetes group. The spectra of cholesterol exhibit similar absorbance peaks to those of glucose within NIR range. PLS modelling results demonstrate that glucose prediction is influenced by cholesterol concentrations in a calibration model. Furthermore, a model expression ( R2 = 0.993) is fitted to quantitatively describe the glucose prediction increment () due to cholesterol concentration (). The results show that glucose prediction accuracy can be improved up to 38.36% by using RWM when using NIRS.ConclusionsThe cholesterol has an effect on blood glucose sensing. RWM is useful to help realize non‐invasive blood glucose sensing by NIRS. Lasers Surg. Med. 47:88–97, 2015. © 2015 Wiley Periodicals, Inc.
Geräte made in China
Authors: Ehrensberger Christian 
Published: January 2015

AbstractAuf der Analytica ging eine ganze Reihe chinesischer Anbieter von Analysengeräten an den Start. Sind sie eine ernsthafte Konkurrenz für etablierte Hersteller? Die Eindrücke in hiesigen Laboratorien, bei Branchengrößen und den Neuankömmlingen selbst sind gemischt.
Lernorte: Garten, Stausee, Bergwerksstollen
Authors: Spitzer Philipp , Krischer Daniela , Gröger Martin 
Published: January 2015

AbstractChemie und Natur sind stärker verwoben, als den meisten Menschen bewusst ist. Ein Freilandlabor, ein mobiles Umweltlabor und weitere Projekte der Arbeitsgruppe Didaktik der Chemie an der Universität Siegen machen diesen Zusammenhang im Chemieunterricht erlebbar.
Otto von Guerickes Windbüchse
Authors: Vollmer Michael , Möllmann Klaus‐Peter 
Published: January 2015

AbstractIm Jahre 1672 beschrieb Otto von Guericke eine Vorrichtung, um Geschosse durch die Druckdifferenz zwischen Luftdruck und zuvor erzeugtem Vakuum zu beschleunigen. Das Grundprinzip dieser Windbüchse entspricht dem moderner Luftgewehre. An einem Nachbau konnten wir mit Hilfe von Hochgeschwindigkeits‐Videos deren Wirkung untersuchen.
Der Shifter
Authors: Loos Andreas 
Published: January 2015

AbstractAls gebürtiger Kalifornier unternimmt der gesuchte Physiker gerne Spaziergänge am Meer. Doch damit ist es vorbei, als er eine deutsche Exilantin heiratet: Die Behörden fürchten, dass er Kontakt mit deutschen U‐Booten aufnehmen könnte. Dass seine Gattin, eine studierte Kunsthistorikerin, soeben vor den Nazis geflohen ist und ihr Ehemann ihre politischen Ansichten teilt, interessiert die Behörden nicht. Stattdessen nehmen sie dem Physiker auch noch sein Kurzwellenradio weg.
Al dente – die Physikerdefinition
Authors: Vilgis Thomas 
Published: January 2015

AbstractPasta wird al dente serviert. Beim Einhalten der angegebenen Kochzeit bleibt der Kern der Nudel hart, Physiker sagen: Er bleibt im Glaszustand. Dieser wirksame Textureffekt macht einen Großteil des Genusses aus. Bei selbst gemachter Pasta funktioniert das übrigens nicht.
Auf der Jagd nach Quantentornados
Authors: Rolles Daniel , Erk Benjamin , Boll Rebecca 
Published: January 2015

AbstractDie hochintensiven und ultrakurzen Lichtpulse moderner Röntgenlaser erlauben es, chemische Reaktionen auf molekularer Ebene zu filmen und Beugungsbilder von Nanokristallen oder einzelnen Viren aufzunehmen, mit denen sich deren Struktur entschlüsseln lässt. Auf diese Weise ist es einer internationalen Gruppe unter Beteiligung des DESY gelungen, Schnappschüsse von Quantenstrudeln in winzigen Tröpfchen suprafluiden Heliums aufzunehmen.
Structural insight into equine lentivirus receptor 1
Authors: Qian Lei , Han Xiaodong , Liu Xinqi 
Published: January 2015

AbstractEquine lentivirus receptor 1 (ELR1) has been identified as a functional cellular receptor for equine infectious anemia virus (EIAV). Herein, recombinant ELR1 and EIAV surface glycoprotein gp90 were respectively expressed in Drosophila melanogaster S2 cells, and purified to homogeneity by Ni‐NTA affinity chromatography and gel filtration chromatography. Gel filtration chromatography and analytical ultracentrifugation (AUC) analyses indicated that both ELR1 and gp90 existed as individual monomers in solution and formed a complex with a stoichiometry of 1:1 when mixed. The structure of ELR1 was first determined with the molecular replacement method, which belongs to the space group P42212 with one molecule in an asymmetric unit. It contains eight antiparallel β‐sheets, of which four are in cysteine rich domain 1 (CRD1) and two are in CRD2 and CRD3, respectively. Alignment of ELR1 with HVEM and CD134 indicated that Tyr61, Leu70, and Gly72 in CRD1 of ELR1 are important residues for binding to gp90. Isothermal titration calorimetry (ITC) experiments further confirmed that Leu70 and Gly72 are the critical residues.
The enzymatic nature of an anonymous protein sequence cannot reliably be inferred from superfamily level structural information alone
Authors: Roche Daniel Barry , Brüls Thomas 
Published: January 2015

AbstractAs the largest fraction of any proteome does not carry out enzymatic functions, and in order to leverage 3D structural data for the annotation of increasingly higher volumes of sequence data, we wanted to assess the strength of the link between coarse grained structural data (i.e., homologous superfamily level) and the enzymatic versus non‐enzymatic nature of protein sequences. To probe this relationship, we took advantage of 41 phylogenetically diverse (encompassing 11 distinct phyla) genomes recently sequenced within the GEBA initiative, for which we integrated structural information, as defined by CATH, with enzyme level information, as defined by Enzyme Commission (EC) numbers. This analysis revealed that only a very small fraction (about 1%) of domain sequences occurring in the analyzed genomes was found to be associated with homologous superfamilies strongly indicative of enzymatic function. Resorting to less stringent criteria to define enzyme versus non‐enzyme biased structural classes or excluding highly prevalent folds from the analysis had only modest effect on this proportion. Thus, the low genomic coverage by structurally anchored protein domains strongly associated to catalytic activities indicates that, on its own, the power of coarse grained structural information to infer the general property of being an enzyme is rather limited.
Bioequivalence of Sandoz methylphenidate osmotic‐controlled release tablet with Concerta® (Janssen‐Cilag)
Authors: Schapperer Elisabeth , Daumann Heike , Lamouche Stéphane , Thyroff‐Friesinger Ursula , Viel François , Weitschies Werner 
Published: January 2015

AbstractThe aim was to assess the bioequivalence of Sandoz methylphenidate osmotic‐controlled release (OCR) tablets (Sandoz [Methylphenidate] MPH OCR) with Concerta®, a methylphenidate formulation indicated for the treatment of attention deficit/hyperactivity disorder (ADHD). Four open‐label, randomized, single‐dose, two‐way crossover bioequivalence studies were conducted in healthy subjects: three fasting studies with 54‐, 36‐ and 18‐mg doses of methylphenidate, and one fed study with the 54‐mg dose. The d‐ and l‐threo‐methylphenidate plasma levels were quantified using liquid chromatographic methods with tandem mass spectrometry (LC MS/MS). Bioequivalence of the formulations was accepted if the 90% geometric confidence intervals of the ratio of least‐squares means of Sandoz MPH OCR to Concerta® of ln‐transformed area under the curve (AUC0–t) and Cmax were within the acceptance range of 80–125%. All studies met the bioequivalence criteria, and 90% geometric confidence intervals for AUC0–t and Cmax were within the predefined range. All plasma concentration time curves for Sandoz MPH OCR under fasting conditions showed a biphasic profile comparable with Concerta®, confirmed by bioequivalence of the partial metrics AUC0–2h, AUC2‐24 h, Cmax(0–2 h) and Cmax(2–24 h). Both products were well tolerated and no relevant differences in the safety profiles were observed. It was concluded that Sandoz MPH OCR is bioequivalent to Concerta® in terms of rate and extent of absorption when administered as a single dose of one extended‐release tablet of 54, 36, or 18 mg under fasting conditions and at a dose of 54 mg under fed conditions.
The Impact of Type of Mesh Repair on 2nd Recurrence After Recurrent Groin Hernia Surgery
Authors: Sevonius D. , Sandblom G. , Agger E. , Smedberg S. , Montgomery A. 
Published: January 2015

AbstractBackgroundAccording to the Swedish Hernia Register (SHR), the reoperation rate is more than doubled after recurrent groin hernia repair compared with primary repair. The aim was to study the impact of type of mesh repair used in recurrent groin hernia surgery on a 2nd recurrence in a population‐based cohort derived from the SHR.Material and methodAll 1st recurrent hernia repairs in the south‐west region of Sweden, registered in SHR between 1998 up to 2007 were included. A questionnaire was sent in 2009. Patients stating a new lump or persisting problems were examined. A 2nd recurrence was identified as a 2nd reoperation or at physical examination. The incidence was analysed comparing anterior mesh repair (AMR) and posterior mesh repairs (PMR) (endoscopic and open).
ResultsEight hundred and fifteen recurrent operations in 767 patents were analysed, 401 AMRs and 414 PMRs. PMR had a lower 2nd recurrence rate compared with AMR (5.6 vs. 11.0 %) (p = 0.025). An increased risk [3.21 (CI 1.33–7.44) (p = 0.009)] of a subsequent 2nd recurrence was seen after anterior index repair followed by AMR and a decreased risk [0.08 (CI 0.01–0.94) (p = 0.045)] after posterior index repair followed by AMR.ConclusionPMR in recurrent groin hernia surgery was associated with a lower 2nd recurrence rate compared to anterior. A posterior approach for 1st recurrent operation is recommended after an anterior index repair and an anterior approach after a posterior index operation.