bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of pinolenic acid‐enriched triacylglycerol from pine nut oil via a two‐step consecutive enzyme reaction: Comparison of acyl donors
Authors: Lee Yu Jin , Park Mi Soon , Kim Byung Hee , Kim Yangha , Kim In‐Hwan 
Published: July 2024

AbstractPinolenic acid (PLA) is a plant‐origin Δ5‐unsaturated polymethylene‐interrupted fatty acid that provides several beneficial health effects to the human body. A two‐step lipase‐catalyzed reaction was carried out to synthesize PLA‐enriched triacylglycerol (TAG) with pine nut oil (PNO) in the present study. In the first step, PLA was efficiently enriched from an initial value of 15–43 mol% in ethyl ester of the reaction mixture after Lipozyme 435‐catalyzed ethanolysis with PNO. In the second step, PLA‐enriched TAG was efficiently synthesized with the fatty acid form via Lipozyme 435‐catalyzed esterification at all temperatures when both ethyl ester form and fatty acid form were compared as acyl donors. The effect of vacuum on the synthesis of PLA‐enriched TAG with glycerol and PLA‐enriched fatty acid was studied. The optimum temperature and vacuum were 60°C, and 50 mmHg, respectively. A maximum TAG conversion of approximately 95% was achieved after 12 h under the optimum conditions.
Día de Muertos: A confluence of gross anatomy, culture, and spirituality
Authors: Herriott Hannah L. , Del Rio Neftali R. , Justin Daniel P. 
Published: July 2024

AbstractIs there room for spirituality and reflection in gross anatomy? While Jesuit institutions have incorporated contemplation and reflection into their curricula for centuries; the integration of reflective practices into the gross anatomy curriculum has gained traction in recent years. Additionally, more emphasis has been placed on diversity, equity, inclusion, and belonging within health professions education. As a Jesuit, Catholic university that is simultaneously classified as a Hispanic‐Serving Institute (HSI), Regis University was well‐positioned to integrate reflective and celebratory activities in honor of Día de Muertos (the Day of the Dead) into a graduate level anatomy course for Doctor of Physical Therapy students. From a communal ofrenda (altar) to a celebration during a lecture to a reflective ceremony honoring body donors in the laboratory, students and faculty constructed a learning environment that incorporated spirituality and culture into anatomy in a thoughtful manner. Furthermore, by seeking student input on the design of this innovative educational experience, each of these activities aided in fostering a sense of cultural and spiritual belonging for Hispanic and Latine students who are historically marginalized in health professions, thereby promoting diversity, equity, inclusion, and belonging through a gross anatomy course.
Solar disk integration polarimeter: An automated disk‐integration full‐Stokes‐vector solar feed for the Potsdam Echelle Polarimetric and Spectroscopic Instrument spectrograph
Authors: Strassmeier Klaus G. , Ilyin Ilya , Woche Manfred , Dionies Frank , Weber Michael , Järvinen Arto , Denker Carsten , Dineva Ekaterina , Verma Meetu , Granzer Thomas , Bittner Wilbert , Bauer Svend‐Marian , Paschke Jens , Önel Hakan 
Published: July 2024

AbstractWe introduce a new solar feed for the PEPSI nighttime spectrograph of the LBT. It enables spectroscopy of the Sun‐as‐a‐star in circular polarization (CP) and linear polarization (LP) with a spectral resolution of 250,000 (≈0.025Å or ) for the wavelength range 383–907 nm. The polarimeter is a dual‐beam design with a modified Wollaston prism as beam splitter and linear polarizer combined with a retractable super‐achromatic retarder. The Wollaston beam diameter is 14 mm and large enough that it does not require a classical telescopic feed anymore. Both polarimetric beams are re‐imaged into respective integration spheres from which two fibers feed the scrambled light to the spectrograph. The system is fully automated in the sense that it finds the Sun in the morning, closes the guider loop, observes a predefined number of individual spectra, and moves to a home position at the end of the day. Among the scientific aims is Zeeman–Doppler imaging of the Sun as a star over the next activity cycle. Our first‐light application detects a clear Stokes‐V/I profile with a full amplitude of on, for example, October 13, 2023, suggesting a solar disk‐averaged line‐of‐sight net magnetic field of +0.37±0.02 G. Comparison of this value with a contemporary full‐disk line‐of‐sight magnetogram suggests an unsigned mean field of about ≈13 G.
Empathy among autistic and non‐autistic adolescents: The importance of informant effects
Authors: Simantov Tslil , Uzefovsky Florina 
Published: July 2024

AbstractEmpathy is the ability to recognize the emotions of others (cognitive empathy) and to share in those emotions while maintaining a self‐other distinction (emotional empathy). Previous research often, but not always, showed that autistic adults and children have lower levels of overall and cognitive empathy than non‐autistic individuals. Yet how empathy manifests during adolescence, a developmental period marked by physiological, social, and cognitive change, is largely unclear. As well, we aimed to compare self versus parents' perceptions regarding adolescents' empathy. To do so, parents (N = 157) of 10–16‐year‐olds (N = 59 autistic) and their children (N = 133) completed empathy questionnaires. Adolescents also completed a measure of mental state recognition (Reading the Mind in the Eyes Test; RMET) and parents reported on their child's autistic traits. The tasks were completed twice ~six months apart. We found that autistic adolescents reported having lower empathic concern and higher personal distress than their non‐autistic peers, whereas parents of autistic adolescents perceived them as having overall lower levels of empathy. Performance on the mental state recognition task of autistic and non‐autistic adolescents' was comparable. The gap between self and parent reports regarding adolescents' empathy was explainable by parent‐reported autistic traits, mainly communication difficulties. Empathy remains stable across the study's two time points. Thus, the findings do not support previous views of autistic people as having less empathy and these are possibly explainable by informant effects.
Evaluation of suppressor behavior of guanidine‐derived metformin and galegine as novel potential drugs for cancer treatment: an in silico study
Authors: Behrouzi Varjovi Mohammad , Asghari‐Zakaria Rasool , Hosseinzadeh Ghader 
Published: July 2024

AbstractThere are some natural products from plants that can prevent and treat disease. Metformin, a derivative of galegine, is the basic drug to treat diabetes. Moreover, this molecule has anticancer properties that inhibit cancer cell growth and proliferation. In this study, the main interactions of galegine and metformin with various cancer‐involved proteins, including mitochondrial alpha‐glycerophosphate dehydrogenase, yeast NADH dehydrogenase, and transforming growth factor‐β1, were surveyed by molecular docking and molecular dynamics simulations. The results showed that each of the proteins makes complexes with the ligands via favorable non‐bonded interactions, especially hydrogen bond interactions. There is greater stability for complexes containing galegine based on the root mean square deviation results. The higher structure compactness is also found in galegine receptors than in metformin receptors. Calculation of ΔGbinding, using the MM/PBSA methodology, shows that the binding energy values for metformin and galegine in interaction with each of the receptors are almost the same, and galegine has similar binding properties with metformin in interaction with the studied protein receptors. Therefore, galegine, a natural ingredient with better binding properties to cancer‐involved proteins than metformin (with various side effects), can be applied as a new drug for cancer treatment.
Protocol for Quantifying All Electrolyte Compositions in Aged Lithium‐ion Batteries
Authors: Zhang Huiyan , Peng Yufan , Zhang Ke , Tang Shijun , Wei Yimin , Liang Jinding , Jin Yanting , Yang Yong 
Published: July 2024

AbstractThe aging of lithium‐ion batteries (LIBs) typically accompanies the degradation of electrolyte, but the relationship between them remains unclear. Therefore, quantifying residual electrolyte in batteries at different states of health (SOH) is a crucial issue. Here, we have developed a comprehensive characterization method to quantitatively analyze the electrolyte salts, solvents, and additives in commercial pouch cell, achieving quantification of all electrolyte compositions with high accuracy. Compared to the reported external standard method used in gas chromatography‐mass spectrometry (GC‐MS), we developed an internal standard method, which offers higher accuracy and reliability, with the maximum error decreased from 9.54 % to 3.48 %. Moreover, the quantitative accuracy of the calibration curves remains unchanged after 2 months. Multi‐instruments analysis is also utilized for the extraction and quantitative analysis of electrolyte in practical battery systems, achieving less than 5 % quantification error for all compositions. With our proposed method, it becomes possible to determine the absolute amounts of all electrolyte compositions, rather than obtaining limited information such as concentration or relative content. It is believed that this protocol of quantifying electrolyte compositions in commercial cells could serve as a baseline for further studies to reveal the relationship between electrolyte degradation and battery aging.
Natural history study of Pseudoachondroplasia: A focus on oral health
Authors: Patel Omkar , Hashmi S. Shahrukh , Chiquet Brett , Hecht Jacqueline T. 
Published: July 2024

AbstractBackgroundPseudoachondroplasia (PSACH) is a rare dwarfing condition characterized by short limbs and fingers, and multiple skeletal abnormalities/complications. There are few natural history studies delineating the medical problems in PSACH leaving a gap in many areas, especially oral health. Our study aimed to obtain information pertaining to oral health and other health‐related problems (pregnancy and childbirth, skeletal health, joint pain) in patients with PSACH.MethodsTo ascertain this information, an online Qualtrics survey was distributed to members of Little People of America, a support group, and through a PSACH online chatroom.ResultsNinety‐nine of 115 surveys were completed and included in the descriptive and multivariable analyses. PSACH individuals regularly sought dental care, but flossing was challenging because of short fingers. Untreated carries (5%), bleeding gums (16%) malocclusion (37%), obstructive sleep apnea (9%), and TMJ disorder (3%) occurred less frequently compared to the general population. Delivery was by Caesarean section in 100% of female respondents who delivered a baby. Bowlegs (74%), scoliosis (43%) and osteoarthritis (36%) were the most common skeletal complications. Joint pain was reported by 85% of respondents.ConclusionsThis study provides novel insights into oral health, pregnancy and childbirth while confirming previously identified skeletal complications in PSACH. Our findings suggest that oral healthcare in PSACH presents unique challenges.
Exosomal lncRNA USP30‐AS1 activates the Wnt/β‐catenin signaling pathway to promote cervical cancer progression via stabilization of β‐catenin by USP30
Authors: Chi Chi , Tang Xiuwu , Liu Wei , Zhou Ying , Jiang Rong , Chen Youguo , Li Min 
Published: July 2024

AbstractCervical cancer (CC) remains a major cause of cancer‐related mortality among women globally. Long noncoding RNAs (lncRNAs) play crucial regulatory roles in various cancers, including CC. This study investigates the function of a novel lncRNA, USP30 antisense RNA 1 (USP30‐AS1), in CC tumorigenesis. We analyzed USP30‐AS1 expression using RT‐qPCR and conducted in vitro loss‐of‐function assays, as well as in vivo assays, to evaluate the effects of USP30‐AS1 silencing on CC cell growth and migration. Additional mechanistic experiments, including RNA pull‐down, RNA immunoprecipitation (RIP), and co‐immunoprecipitation (Co‐IP) assays, were performed to elucidate the regulatory mechanisms influenced by USP30‐AS1. We discovered that USP30‐AS1 is overexpressed in CC tissues and cells. Silencing USP30‐AS1 significantly reduced cell proliferation, migration, invasion, and tumor growth. Moreover, USP30‐AS1 was found to modulate the expression of ubiquitin‐specific peptidase 30 (USP30) by sponging microRNA‐2467‐3p (miR‐2467‐3p) and recruiting the FUS RNA binding protein (FUS), thereby stabilizing β‐catenin and activating the Wnt/β‐catenin signaling pathway. These findings suggest that USP30‐AS1 enhances CC cell growth and migration through the miR‐2467‐3p/FUS/USP30 axis, highlighting its potential as a biomarker for CC.
Synthetic biology for Monascus: From strain breeding to industrial production
Authors: Zhou Junping , Pan Qilu , Xue Yinan , Dong Yaping , Chen Yihong , Huang Lianggang , Zhang Bo , Liu Zhi‐Qiang , Zheng Yuguo 
Published: July 2024

AbstractTraditional Chinese food therapies often motivate the development of modern medicines, and learning from them will bring bright prospects. Monascus, a conventional Chinese fungus with centuries of use in the food industry, produces various metabolites, including natural pigments, lipid‐lowering substances, and other bioactive ingredients. Recent Monascus studies focused on the metabolite biosynthesis mechanisms, strain modifications, and fermentation process optimizations, significantly advancing Monascus development on a lab scale. However, the advanced manufacture for Monascus is lacking, restricting its scale production. Here, the synthetic biology techniques and their challenges for engineering filamentous fungi were summarized, especially for Monascus. With further in‐depth discussions of automatic solid‐state fermentation manufacturing and prospects for combining synthetic biology and process intensification, the industrial scale production of Monascus will succeed with the help of Monascus improvement and intelligent fermentation control, promoting Monascus applications in food, cosmetic, agriculture, medicine, and environmental protection industries.
Method validation, residue behaviour and dietary risk assessment of insecticides (cyantraniliprole, acetamiprid, flubendiamide and its metabolite, des‐iodo flubendiamide) in or on broccoli using LC–MS/MS
Authors: Sharma Sakshi , Katna Sapna , Sharma Ajay , Istatu Pankaj Sharma , Devi Nisha , Kumar Arvind , Singh Shubhra 
Published: July 2024

AbstractResidue behaviour and dietary risk assessment of cyantraniliprole, flubendiamide and acetamiprid in broccoli were carried out using the QuEChERS (quick, easy, cheap, effective, rugged and safe) technique coupled with LC–MS/MS. The QuEChERS technique was validated on parameters such as linearity, accuracy, precision, robustness, matrix effects, limit of quantification (LOQ), specificity, retention time and ion ratio as per SANTE (Directorate General for Health and Food Safety) guidelines to attest to the specificity, accuracy and precision of the analytical method in estimating insecticide residues in and on broccoli heads and cropped soil. The LOQ of the method for all three insecticides was 0.01 mg/kg. The initial deposits of cyantraniliprole, flubendiamide and acetamiprid reduced to half of its concentration in 1.873–2.354, 1.975–2.484 and 1.371–1.620 days, respectively. No residues were detected in broccoli‐cropped soil at harvest time (30 days after last spray). The proposed maximum residue limits (MRLs) of 1.5, 0.5–0.9 and 2.0–3 mg/kg for cyantraniliprole, flubendiamide and acetamiprid were calculated using the Organisation for Economic Co‐operation and Development MRL calculator. The acute and chronic dietary risk assessment of the tested insecticides identified no appreciable dietary risk to the Indian population from the consumption of broccoli heads. The findings of no dietary risk highlight the importance of informed pesticide usage in broccoli and the proposed MRL derived from this study offers crucial guidelines for the regulatory authorities, ensuring the safety of broccoli consumption.
Peripheral sensory nerve regeneration: Novel target in bone tissue engineering
Authors: Xiao Lan , Liu Jiaying , Yan Fuhua , Xiao Yin 
Published: July 2024

AbstractSynthetic biomaterials are emerging candidate solutions for treating large bone defects. However, the clinical performances of most synthetic materials are not satisfactory, with the need for improvement in design and synthesis. Although bone is highly innervated, the central role during healing of the peripheral nervous system, and in particular sensory nerves (SNs), has only recently been acknowledged. SNs can improve osteogenic differentiation of bone marrow stem/stromal cells through neurotransmitters and peptides; the interplay between SNs and the vascular system also facilitates vascular network reconstruction, indirectly facilitating bone healing. These factors suggest the importance of SNs in bone healing, a vital point that has been overlooked in bone biomaterial design until very recently. SN regeneration represents a novel direction in the development of biomaterials for bone regeneration. The current perspective paper summarizes the cellular and molecular mechanisms under the regulatory influence of SNs in the bone healing process and outlines the recent advances in biomaterials for innervated bone tissue regeneration. This establishes potential future directions for bone engineering biomaterial design.
The local range of natural cosmetics: Consumer perceptions and assessments in Germany
Authors: Ruf Julia , Menrad Klaus , Emberger‐Klein Agnes 
Published: July 2024

AbstractNatural cosmetics offer the opportunity to reduce the environmental burden caused by the consumption of cosmetic products. This qualitative study analyzes the local range of natural cosmetics and related consumer perceptions and assessments in two case cities in Germany by applying a mixed‐method approach of store checks and the photovoice method. Results of the study show that there is only a limited match of the actual range and consumer perception for some types of retail businesses and a mismatch for product categories. Additionally, participants showed little awareness of the most common natural cosmetic certifications. Consumer assessments about the range of natural cosmetics were influenced by factors such as the arrangement of products in the shops and consumers' experience and knowledge about natural cosmetic brands. Retailers can use the study results in arranging natural cosmetics accordingly; companies can use it to emphasize branding, and policy‐makers can increase consumer knowledge about natural cosmetics.
What drives responsible consumption in collectivistic developing countries? An analysis of Vietnamese consumers' motivations with value–belief–norm theory
Authors: Nguyen Thuy‐Phuong , Dekhili Sihem 
Published: July 2024

AbstractThis study explores the motivations driving responsible consumption in Vietnam, a collectivistic and populous Asian developing country. Specifically, on the basis of value‐belief‐norm (VBN) theory, it focuses on the direct effects of personal values (biospheric, altruistic, and egoistic values) on green product purchase intent. The empirical collectivistic cultural context of Vietnam was utilized, with data collected from 806 consumers across 35 cities. The study reveals that biospheric values predominantly drive purchase intent for green products; however, the magnitude of egoistic values, particularly health concerns, varies significantly across product categories. Regarding altruistic values, they do not appear to be salient among Vietnamese consumers. Additionally, the environmental motive can act as a mediator between health motive and purchase intent. This indirect effect is particularly strong in the case of organic food. Based on these findings, the authors derive managerial and academic implications.
Effect of artificial lung fiber bundle geometric design on micro‐ and macro‐scale clot formation
Authors: Lai Angela , Omori Natsuha , Napolitano Julia E. , Antaki James F. , Cook Keith E. 
Published: July 2024

AbstractThe hollow fiber membrane bundle is the functional component of artificial lungs, transferring oxygen to and carbon dioxide from the blood. It is also the primary location of blood clot formation and propagation in these devices. The geometric design of fiber bundles is defined by a narrow set of parameters that determine gas exchange efficiency and blood flow resistance, principally: fiber packing density, path length, and frontal area. These same parameters also affect thrombosis. This study investigated the effect of these parameters on clot formation using 3D printed flow chambers that mimic the geometry and blood flow patterns of fiber bundles. Hollow fibers were represented by an array of vertical micro‐rods (380 μm diameter) arranged with three packing densities (40%, 50%, and 60%) and two path lengths (2 and 4 cm). Blood was pumped through these devices corresponding to three mean blood flow velocities (16, 20, and 25 cm/min). Results showed that (1) clot formation decreases dramatically with decreasing packing density and increasing blood flow velocity, (2) clot formation at the outlet of the fiber bundle enhances deposition upstream, and consequently (3) greater path length provides greater clot‐free fiber surface area for gas exchange than a shorter path length. These results can help guide the design of less thrombogenic, more efficient artificial lung designs.
Synthesis, DFT Calculations, and Biological Studies of New 2‐Cyano‐3‐(Naphthalene‐1‐yl) Acryloyl Amide Analogues as Anticancer Agents
Authors: Hekal Mohamed H. , Abdalha Abdelaal A. , Farag Hanaa , Ali Amira T. 
Published: July 2024

AbstractA set of novel naphthalene derivatives was synthesized via investment of the electrophilic reaction center of the easily obtainable starting substance, 2‐cyano‐3‐(naphthalen‐1‐yl)acryloyl chloride (1), with various nitrogen nucleophiles and assessed as potential antitumor agents. The chemical structures of these derivatives were completely specified using several spectral and elemental analyses. The antiproliferative efficacy of the discovered compounds against the human cancer cell lines HepG2 and MCF‐7 was investigated. Compounds 12b and 9 have more potent anticancer activity versus MCF‐7 breast cancer. DFT calculations for the synthesized compounds were studied to determine molecular geometry, frontier orbital analysis, and molecular electrostatic potential. Compound 2 has the lowest energy gap, the highest softness, and the lowest hardness molecule. Also, the electrophilicity values of the studied molecules provide evidence for their biological effectiveness, as compound 9 had significant antiproliferative activity and a high value of electrophilicity (ω) (0.190 eV).