bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-cancer analysis of Sp1 with a focus on immunological roles in gastric cancer
Authors: Zhou Yang , Luo Zhenzhen , Guo Jinfeng , Wu Lixia , Zhou Xiaoli , Huang Junjie , Huang Daijia , Li Xiao , Duan Qiuhua , Chang Jianhua , Gong Libao , Hang Junjie 
Published: August 2024

Abstract

Background
Sp1, a transcription factor, plays a pivotal role in tumorigenesis across diverse cancers. However, its comprehensive pan-cancer analyses and immunological roles in gastric cancer (GC) remain inadequately elucidated.
Methods
Through a comprehensive analysis utilizing bioinformatics tools and datasets from TCGA, GEO, and THPA, we examined the multifaceted role of Sp1. Expression profiles were assessed across cell lines, tissues, and tumors, alongside exploration of genetic alterations, DNA methylation, and protein phosphorylation. Its associations with immune infiltration, tumor mutational burden, and immune checkpoint signaling were investigated. Additionally, single-cell transcriptome data showed its expression in different immune cells in GC. Validation of correlations between Sp1 and immune microenvironment in GC was performed using immunohistochemistry and multiple immunofluorescence in an immunotherapy-treated patient cohort. The prognostic value of Sp1 in GC receiving immunotherapy was evaluated with Cox regression model.
Results
Elevated Sp1 levels were observed in various cancers compared to normal tissues, with notable prominence in gastric cancer. High Sp1 expression correlated with advanced stage, poor prognosis, elevated tumor mutational burden (TMB), and microsatellite instability (MSI) status, particularly in GC. Sp1 levels also correlated with CD8 + T cell and M1 phenotype of tumor-associated macrophages infiltration. Furthermore, GC patients with higher Sp1 levels exhibited improved response to immunotherapy. Moreover, Sp1 emerged as a prognostic and predictive biomarker for GC patients undergoing immunotherapy.
Conclusions
Our pan-cancer analysis sheds light on Sp1's multifaceted role in tumorigenesis and underscores its potential as a prognostic and predictive biomarker for GC patients undergoing immunotherapy.
Borderline Ovarian Tumors: Recurrence Patterns and Management
Authors: Tunç Mehmet , Akıllı Hüseyin , Günakan Emre , Haberal Nihan , Haberal Ali , Ayhan Ali 
Published: August 2024

Abstract

Purpose: We aimed to evaluate the factors associated with disease recurrence, recurrence patterns, and obstetric outcomes of borderline ovarian tumors. The main outcome was prognostic factors for disease recurrence. The secondary outcomes were recurrence sites and obstetric results.
Methods: This study included patients diagnosed with BOT in Başkent University. Data was obtained from patient files and hospital records. Histopathological results were re-evaluated based on the new 2020 WHO classification. Risk factors for disease recurrence were evaluated for early-stage and advanced-stage diseases. Survival was measured from the time of diagnosis.
Results: A total of 142 patients were included. The median follow-up time was 100.5 months. Recurrence occurred in 24(16.9%) patients and the 5-year RFS 86.3% and no deaths were recorded. The main recurrence site of the tumor was the same ovary (12/24, 50%). In multivariate analysis, cystectomy was found as a risk factor for recurrence in the early stage (HR:4.28; 95%CI: 1.40 – 13.08, p:0.011). One patient’s tumor showed malignant transformation (1/24, 4.17%). The pregnancy rate was 76.7% among 43 patients who attempted to conceive. There was no difference in obstetric outcomes between USO and cystectomy (p:0.223).
Conclusion: The risk of recurrence in patients with BOT was higher in patients who underwent cystectomy and obstetric outcomes were similar between cystectomy and USO. In this study, most recurrences occurred in the ovaries. Therefore, fertility-sparing appears to be an appropriate choice for young women with satisfactory obstetric outcomes even in the advanced stage.
Effects of calcium phosphate and phosphorus-dissolving bacteria on microbial structure and function during Torreya grandis branch waste composting
Authors: Yu Chenliang , Guan Yuanyuan , Wang Qi , Li Yi , Wang Lei , Yu Weiwu , Wu Jiasheng 
Published: August 2024

Abstract

Background
To investigate the effects of phosphorus solubilizing microorganisms and calcium phosphate on the composting of Torreya grandis branches and leaves, as well as to explain the nutritional and metabolic markers related to the composting process.
Methods
In this study, we employed amplicon sequencing and untargeted metabolomics analysis to examine the interplay among phosphorus (P) components, microbial communities, and metabolites during T. grandis branch and leaf waste composting that underwent treatment with calcium phosphate and phosphate-solubilizing bacteria (Burkholderia).
Results
The results indicated that Burkholderia inoculation and calcium phosphate treatment affected the phosphorus composition, pH, EC, and nitrogen content. Furthermore, these treatments significantly affected the diversity and structure of bacterial and fungal communities, altering microbial and metabolite interactions. The differential metabolites associated with lipids and organic acids and derivatives treated with calcium phosphate treatment are twice as high as those treated with Burkholderia in both 21d and 42d. The results suggest that calcium phosphate treatment alters the formation of some biological macromolecules.
Conclusion
These results extend our comprehension of the coupling of matter transformation and community succession in composting with the addition of calcium phosphate and phosphate-solubilizing bacteria.
Numerical analysis of microwave and radiofrequency ablations: A novel design for electrode-based radiofrequency ablation
Authors: Hamzavi Fahimeh , Karimi Mohammad Javad , Bahadoran Mahdi 
Published: August 2024

Abstract

Thermal ablation is atreatment modality for liver cancer that utilizes heat to destroy cancerous tissues. The numerical analysis of thermal ablation processes in liver tissue was studied using two different methods: microwave ablation (MWA) and radiofrequency ablation (RFA). The effectiveness of both methods and the thermal characteristics of tumor ablation, including temperature distribution, temporal variation intemperature, volume of ablation, and destruction of cancerous tissue, were investigated. MWA requires less time for complete ablation than RFA and can generate larger ablation zones. Additionally, a novel electrode-based design for RFA, we call it Norfolk pine electrode (NPE), which focuses on ablating large and spherical tumors, has been proposed and analyzed. To the best of our knowledge, the conventional RFA designs, which utilize the Christmas tree electrode and umbrella electrode, are effective in ablating tumors smaller than 3 cm . However, they fail to completely eradicatespherical tumors. Interestingly, our proposed NPE claims the ability to fully ablate spherical tumors, even those larger than 3 cm . This NPE could be a promising and practical advancement in the realm of tumor treatment.
A food-borne outbreak of gastroenteritis caused by norovirus GII in a university located in Guangzhou City, China
Authors: Hui Wang , Xie Huaping , Chen Changming , LI Nengjiu , Zhen Ruonan , Lu Jianyun , Li Xiaoning 
Published: August 2024

Abstract

In order to determine the causative pathogen and source of infection of an acute gastroenteritis outbreak in a university located in Guangzhou City, China, and summarize the lessons learned from food safety risk management.A series of epidemiological investigations were conducted in the university, including investigations of cases, food, drinking water, and the environment. A 1:4 matched case-control study was carried out to explore the risk factors for infection. Reverse transcriptase PCR was performed on all suspected cases for norovirus detection. Phylogenetic analysis was conducted to confirm pathogen typing. A total of 431 suspected cases were identified in this outbreak, of which 216 were laboratory-confirmed cases. The main clinical symptoms observed in the cases were diarrhea (62.6%) and vomiting (60.8%). A retrospective case-control study with a ratio of 1:4 was conducted. The results of multivariate analysis showed that four factors were significantly associated with the outbreak, including contact with patients' vomit or stool, presence of cases in the same dormitory, dining at Canteen B and Canteen E respectively. The odds ratios (OR) and their corresponding 95% confidence intervals (CI) for these factors were as follows: 15.587 (2.188 ~ 111.054), 3.166 (1.595 ~ 6.281), 3.86 (1.497 ~ 9.956), and 9.456(4.785 ~ 18.686), respectively. Phylogenetic analysis indicated that all eight specimens tested positive for norovirus GII.4 strains including seven confirmed outbreak cases and one food handler.Based on the environmental investigation, case-control study and laboratory test results, it was concluded that the outbreak was caused by kitchen workers infected by norovirus through contact. The health surveillance and hygiene practice of kitchen workers still need to be strengthened in the future.
A Study On Optimizing The Organisation System With Focus On Mental Wellbeing, Substance Abuse, Counterproductive Work Behaviour, Resilience And Stress, Using Structured Equation Modeling
Authors: Deka Prateek , Singh Gurinder , Sharma Chandra Shekhar , Verma Pranay , Kapoor Shikha 
Published: August 2024

Abstract

Inspired by the principles of Systems Theory, this study explores the modern workplace, as a multifaceted and ever-evolving organisation system in which mental wellbeing, stress, resilience, counterproductive work behavior, and substance abuse are interrelated, impacting one another via feedback loops. Thus, our aim was to evaluate a management framework that addresses how these constructs are connected as well as how to manage them within the workplace. A survey tool with standardized questionnaires was used to collect data from 446 employees. The measurement model demonstrated construct reliability and validity, whereas the structural model examined the strength of linkages. Higher resilience reduced stress, boosting mental wellbeing which acted as a stabilizing influence against substance abuse and counterproductive work behavior. This study highlights the complicated interplay between these variables and emphasizes the critical significance of mental wellbeing in maintaining balance for organizational success.
Workplace interpersonal relationships and turnover intentions among care workers in older adult care facilities: a mediation analysis comparing direct and mediated effects via psychological distress
Authors: Takeda Shinya , Fukuzaki Toshiki 
Published: August 2024

Abstract

Background
This study aimed to investigate the relationship between workplace interpersonal relationships, psychological distress, and turnover intentions among care workers working in older adult care facilities.
Methods
An anonymous online survey among 811 care workers in Tottori Prefecture, Japan, was conducted between November and December 2023. The data were collected using the Workplace Interpersonal Problems Scale for care workers (WIPS), the K6 scale, the Turnover Intention Scale and basic attributes. Responses were obtained from 254 participants with a response rate of 31.3%.
Results
The results of the mediation analysis for the WIPS subscale which was adjusted for basic attributes show that insufficient communication (direct effect, 0.319; mediated effect, 0.194), a sense of unfair workload (direct effect, β = 0.314; mediated effect, β = 0.190), and different attitudes to care work (direct effect, β = 0.278; mediated effect, β = 0.204) had stronger direct effect on turnover intention, whereas difficulty guiding subordinates/new staff (direct effect, β = 0.207; mediated effect, β = 0.219) and labeling (direct effect, β = 0.198; mediated effect, β = 0.211) had slightly stronger mediated effect on turnover intentions through psychological distress. For bullying (direct effect, β = 0.207; mediated effect, β = 0.204), the direct and mediated effects on turnover intention were nearly equal.
Conclusions
This study revealed that some workplace interpersonal problems have a stronger direct effect on turnover intentions, whereas others have a stronger mediating effect on turnover intentions through psychological distress. Workplace interpersonal problems are predictors of turnover intentions among care workers working in elderly care facilities, and different workplace interpersonal problems should be targeted for intervention to reduce turnover intentions.
The Protective Effect of Cysteine Peptides on UV-B-Induced Skin Deterioration in Healthy People: A Randomized, Placebo-controlled, Double-blind, Parallel- group Study
Authors: Sakuma Ayako , Kai Yumiko , Yamasaki Yoshimitsu , Tanaka Tomoya , Sakurai Takanobu 
Published: August 2024

Abstract

The oral administration of antioxidants may suppress UV-B-induced skin damage. HITHION™ YH-15, the extract of Torula yeast (Cyberlindnera jadinii), is rich in cysteine-containing peptides such as reduced and oxidized glutathione (GSH and GSSG), γ-glutamylcysteine (γ-Glu-Cys), and cysteinylglycine (Cys-Gly). These four constituents are termed cysteine peptides. In this study, we investigated the protective effects of cysteine peptides against UV-B in a randomized, placebo-controlled, double-blind, parallel-group study. A total of 90 healthy males and females aged 30–59 years were enrolled and randomized into two groups of 45 individuals each (cysteine peptides (48 mg/day) and placebo). Changes in UV-B-induced (1) erythema and (2) pigmentation were compared between groups after 5 weeks of test food intake. The minimal erythema dose (MED) significantly increased (*p = 0.019) in the cysteine peptides group compared to that in the placebo group, indicating suppression of UV-B-induced erythema. ΔL* value significantly increased (***p < 0.0001) in the cysteine peptides group compared to that in the placebo, indicating pigmentation suppression. We demonstrated that oral administration of cysteine peptides suppresses UV-B-induced erythema and pigmentation through multiple mechanisms. Thus, cysteine peptides may find use as nutricosmetics for maintaining skin health and well-being. (UMIN Clinical Trials Registry ID: UMIN 000050157)
In Vitro and In Vivo Vein Assessment of a Novel Vein Visualizing Device to Improve First-Time Peripheral Venous Access
Authors: Liddelow Michael D. , Ho Phuoc Hao , Boyce Cara A. , Redknap Matthew D. , Hansen Ellaby L. , Buckley Nicholas M. , Arenson Katherine , Carr Peter J. , Doyle Barry J. , Bappoo Nikhilesh 
Published: August 2024

Abstract

Inserting needles into veins is fundamental to medical care with up to 90% of inpatients requiring a peripheral intravenous catheter/cannula (PIVC) during their stay. Yet 40%-50% of PIVC insertions fail on the first attempt. Here, we present an easy-to-use novel vein visualizing ultrasound prototype device and data from in vitro and in vivo performance. Our prototype’s locational accuracy in simulated forearm veins is 0.16mm ±1.63mm (s.d.) (97.8% agreement to the ground truth, p<.001), across variations of vein diameter (3-5mm), depth (10-20mm), and velocity (10-100mm/s). Usability trials conducted with nine clinicians found that 100% of users were able to handle the prototype in a sterile manner with minimal assistance. In 80 forearm scans of 40 volunteers, sensitivity was excellent to both find veins (94%). In comparison, sensitivity of vein finding using landmark technique with torniquet (visible 46% and palpable 74%) were far inferior. The prototype is a novel ultrasound device which empowers clinicians to detect and visualize well-perfused veins at depth in the coronal view of vein pathways whilst enabling, ultra portability, accessibility and ease of use.
Evaluation of Therapeutic Outcomes for Intraoperative MCL Injury in Subjects Undergoing Total Knee Arthroplasty: a Cohort Study
Authors: Shirouei Sepehr , Karimi Mehdi , Ghorbani-Amjad Gholamreza , Majidi Morteza , Shirsalimi Niyousha , Moradi Abbas 
Published: August 2024

Abstract

Background
Total knee arthroplasty (TKA) is an effective procedure for replacing damaged knee joints, recommended when conservative treatments fail. Intraoperative medial collateral ligament (MCL) injuries are a known complication of TKA, potentially impacting patient recovery and overall surgical outcomes. Effective management of these injuries is crucial to ensuring optimal function and stability of the knee postoperatively. This study aims to evaluate the therapeutic outcomes for patients who sustained intraoperative MCL injuries during TKA, focusing on recovery, stability, and the need for additional interventions.
Material and Methods
In this prospective cohort study, over 2 years, 553 patients with advanced severe knee arthritis underwent TKA, and only 13 patients had intraoperative MCL injury. After surgery, patients followed a partial weight-bearing protocol with a locked knee brace for two weeks, followed by physiotherapy to restore motion over six weeks gradually. The patients were followed up for 12 months. The International Knee Documentation Committee (IKDC) score was used to evaluate patients before and after surgery, and collected data was analyzed.
Results
None of the patients who experienced intraoperative MCL injury showed any knee instability during the 12-month follow-up period. The statistical analysis revealed no significant relationship between MCL injury and gender (P = 0.786). The mean pain score decreased from 90 preoperatively to 3.8 postoperatively, indicating a statistically significant improvement in knee pain (P < 0.001). No significant association was found between the IKDC score in intraoperative MCL injury and patient gender (P = 0.806). A comparison of the mean IKDC scores post-surgery between individuals who had intraoperative MCL injury (77.78) and received treatment and the control group without intraoperative MCL injury (80.00) revealed that MCL injury, when treated, had no significant effect on the IKDC score (P = 0.141). The mean IKDC score increased from 28.2 preoperatively to 77.8 postoperatively, indicating a statistically significant improvement in knee function (p-value < 0.001).
Conclusion
When MCL injury is treated intraoperatively, it has no significant effect on the IKDC score and significantly improves knee function. MCL injury during TKA, managed with primary repair and reinforcement with hamstring autograft or reattachment to the bone using pull-out techniques followed by six weeks of knee brace use postoperatively, is associated with favorable and treatable outcomes.
SADCL-Net: Sparse-driven Attention with Dual-Consistency Learning Network for Incomplete Multi-view Clustering
Authors: Xue Sicheng , Zhu Changming 
Published: August 2024

Abstract

In recent years, various deep learning-based methods have been proposed to address the problem of incomplete multi-view clustering. However, these methods still face two major challenges: firstly, due to the incompleteness of views, they are susceptible to noise and irrelevant features, leading to redundant and untargeted learned features; secondly, although these methods attempt to leverage the consistent information across different views for cross-view learning, they often overemphasize consistency, thereby neglecting inter-view differences. To address these issues, this paper proposes a novel method: Sparse-driven Attention with Dual-Consistency Learning Network for Incomplete Multi-view Clustering (SADCL-Net). Specifically, we utilize a sparsity-constrained self-attention module to effectively capture the intrinsic sparsity and local salient information of the data, thereby highlighting key features. Subsequently, we design a Dual-Consistency Learning module, which incorporates a joint entropy term into the consistency loss function to balance the consistency and difference information across views, thereby optimizing the contribution of different views to the final clustering results. Additionally, to avoid potential issues arising from imputing missing data, we integrate feature projection and alignment modules. Our proposed method has been extensively evaluated on four widely-used multi-view datasets, with results prominently demonstrating the significant advantages of SADCL-Net in terms of clustering performance.
Evaluation of the Health Literacy Curriculum at a Southeastern Medical School
Authors: Albertson Faith , Kennedy Ann Blair , Taylor Shannon , Natafgi Nabil 
Published: August 2024

Abstract

Background
Health literacy (HL) is crucial for making informed health decisions. Over one-third of US adults have limited HL, leading to adverse health outcomes. Despite its importance, HL education lacks standardization in medical training. This study evaluates the University of South Carolina School of Medicine Greenville’s (USC SOMG) HL curriculum to propose recommendations for HL instruction.
Methods
A convergent parallel mixed methods design was used to assess the HL curriculum through a curriculum review, student survey, and faculty interviews. The study utilized thematic analysis for qualitative data and statistical analysis for quantitative data, focusing on prior and current HL training, confidence in HL application, and perceptions of HL education.
Results
The curriculum at USC SOMG incorporates active learning strategies, emphasizing HL, and patient communication. Most participants reported high confidence in their HL knowledge and skills. The preferred teaching methods were hands-on clinical interactions, observing clinical interactions, and interactive lessons. Barriers to using HL interventions included time constraints and lack of real-world experience. Faculty recommended time prioritization and collaborative strategies to overcome these barriers.
Conclusions
USC SOMG’s HL curriculum combines didactic and active strategies longitudinally, preparing students to feel confident in their ability to engage with patients experiencing LHL. This demonstrates the curriculum’s strength and potential as a model for other schools. However, for broader implementation, standardized requirements and competency-based assessments are recommended to ensure consistent HL education across medical programs, focusing on practical application and overcoming identified barriers. This could markedly enhance patient outcomes by equipping future providers with essential HL skills.