bluuebunny/crossref_metadata_2025_split huggingface dataset:
A preliminary investigation into the impact of soft tissue augmentation-based periodontal phenotype modification therapy for patients exhibiting class III decompensation
Authors: Li Mengdi , Liu ZhiXu , Yang Xiao , Zhu Min , Ni Jing 
Published: August 2024

Abstract
Background
Patients with skeletal angle Class III malocclusion usually have inadequate hard and soft tissue volume at the mandibular anterior teeth. The labial proclination at the teeth may lead to gingival recession. The purpose of this study was to explore whether periodontal phenotype modification therapy with soft tissue augmentation (PhMT-s) can prevent gingival recession in these patients.

Methods
Four patients with skeletal Class III malocclusion and a thin periodontal phenotype underwent surgical-orthodontic treatment. Prior to tooth movement, they underwent a minimally invasive vestibular incision with subperiosteal tunnel access combined with autogenous connective tissue grafts for periodontal phenotype modification with soft tissue augmentation (PhMT-s). The labial gingival thickness of the anterior mandibular teeth was measured at three distinct levels: at the cementoenamel junction (GT0), 3 mm apical to the CEJ (GT3), and 6 mm apical to the CEJ (GT6). These measurements were taken at baseline, three months following PhMT-s, and after tooth decompensation. Additionally, a biopsy sample was obtained from the PhMT-s site of one patient. All sections were subsequently stained using hematoxylin and eosin, Masson trichrome, Sirius Red, and immunohistochemistry.

Results
The thickness of the labial gingiva was increased about 0.42 to 2.00 mm after PhMT-s. At the end of pre-orthognathic surgical orthodontic treatment, the thickness of the labial gingiva was increased about − 0.14 to 1.32 mm compared to the baseline and no gingival recession occurred after the pre-orthognathic surgical orthodontic treatment. The histologic results demonstrated that the grafts obtained from the PhMT-s site exhibited increased deposition of collagen fibers. Moreover, the proportion of type III collagen increased and the grafts displayed significantly reduced positive expression of CD31 and OCN.

Conclusions
PhMT-s increased the thickness of the soft tissue, stabilizing the gingival margin for teeth exhibiting a thin periodontal phenotype and undergoing labial movement. This is attributed to the increased deposition of collagen fibers.
Immediate rehabilitation of a palatal defected edentulous patient by implant-supported overdenture: a case report
Authors: Xin Baojian , Liu Xin 
Published: August 2024

Abstract
Background
Immediate rehabilitation is a considerable therapeutic challenge but is necessary for edentulous patients with oronasal fistulas, especially those with inadequate residual bone and a history of radiotherapy.

Case presentation
We report a rare case of a 63-year-old patient who was missing the majority of his maxillary teeth and who had a defect due to palatal mucoepidermoid carcinoma resection. The patient also received radiotherapy twice within one year postoperatively. An implant-supported prosthesis with an obturator was fabricated immediately.

Conclusion
This technique improved patients’ oral function, enhanced the aesthetic effect, and increased their confidence.
Tumor-derived GLI1 promotes remodeling of the immune tumor microenvironment in melanoma
Authors: Giammona Alessandro , De Vellis Chiara , Crivaro Enrica , Maresca Luisa , Amoriello Roberta , Ricci Federica , Anichini Giulia , Pietrobono Silvia , Pease David R. , Fernandez-Zapico Martin E. , Ballerini Clara , Stecca Barbara 
Published: August 2024

Abstract
Background
Melanoma progression is based on a close interaction between cancer cells and immune cells in the tumor microenvironment (TME). Thus, a better understanding of the mechanisms controlling TME dynamics and composition will help improve the management of this dismal disease. Work from our and other groups has reported the requirement of an active Hedgehog-GLI (HH-GLI) signaling for melanoma growth and stemness. However, the role of the downstream GLI1 transcription factor in melanoma TME remains largely unexplored.

Methods
The immune-modulatory activity of GLI1 was evaluated in a syngeneic B16F10 melanoma mouse model assessing immune populations by flow cytometry. Murine polymorphonuclear myeloid-derived suppressor cells (PMN-MDSCs) were differentiated from bone marrow cells and their immunosuppressive ability was assessed by inhibition of T cells. Conditioned media (CM) from GLI1-overexpressing mouse melanoma cells was used to culture PMN-MDSCs, and the effects of CM were evaluated by Transwell invasion assay and T cell inhibition. Cytokine array analysis, qPCR and chromatin immunoprecipitation were performed to explore the regulation of CX3CL1 expression by GLI1. Human monocyte-derived dendritic cells (moDCs) were cultured in CM from GLI1-silenced patient-derived melanoma cells to assess their activation and recruitment. Blocking antibodies anti-CX3CL1, anti-CCL7 and anti-CXCL8 were used for in vitro functional assays.

Results
Melanoma cell-intrinsic activation of GLI1 promotes changes in the infiltration of immune cells, leading to accumulation of immunosuppressive PMN-MDSCs and regulatory T cells, and to decreased infiltration of dendric cells (DCs), CD8 + and CD4 + T cells in the TME. In addition, we show that ectopic expression of GLI1 in melanoma cells enables PMN-MDSC expansion and recruitment, and increases their ability to inhibit T cells. The chemokine CX3CL1, a direct transcriptional target of GLI1, contributes to PMN-MDSC expansion and recruitment. Finally, silencing of GLI1 in patient-derived melanoma cells promotes the activation of human monocyte-derived dendritic cells (moDCs), increasing cytoskeleton remodeling and invasion ability. This phenotype is partially prevented by blocking the chemokine CCL7, but not CXCL8.

Conclusion
Our findings highlight the relevance of tumor-derived GLI1 in promoting an immune-suppressive TME, which allows melanoma cells to evade the immune system, and pave the way for the design of new combination treatments targeting GLI1.
Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis
Authors: Hou Yuyong , Guo Zhile , Liu Zhiyong , Yan Suihao , Cui Meijie , Chen Fangjian , Wang Weijie , Yu Longjiang , Zhao Lei 
Published: August 2024

AbstractAstaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.
Sustainable machining of AISI4140 steel: a Taguchi-ANN perspective on eco-friendly metal cutting parameters
Authors: Jadhav Pankaj Krishnath , Sahai R. S. N. 
Published: August 2024

AbstractThis work explores environmentally conscious machining practices for AISI4140 steel through Taguchi analysis. The study employs a design of experiments (DOE) approach, focusing on cutting speed, depth of cut, and coolant type as parameters. Taguchi’s L9 orthogonal array facilitates systematic experimentation, and the results are analyzed using MINITAB 17 software. Signal-to-noise ratios (SNR) are utilized to establish optimum operating conditions, evaluate individual parameter influences, and create linear regression models. The experiments reveal neem oil with graphene coolant as an eco-friendly solution, addressing health and environmental concerns. Main effects plots visually represent the impact of parameters on machining quality. Additionally, regression and artificial neural network (ANN) models are compared for surface roughness prediction, with ANN showing superior performance. The findings advocate for optimized cutting conditions, emphasizing material conservation, enhanced productivity, and eco-friendly practices in AISI4140 steel machining. This research contributes valuable insights for industries seeking sustainable machining solutions.
From soil to sequence: filling the critical gap in genome-resolved metagenomics is essential to the future of soil microbial ecology
Authors: Anthony Winston E. , Allison Steven D. , Broderick Caitlin M. , Chavez Rodriguez Luciana , Clum Alicia , Cross Hugh , Eloe-Fadrosh Emiley , Evans Sarah , Fairbanks Dawson , Gallery Rachel , Gontijo Júlia Brandão , Jones Jennifer , McDermott Jason , Pett-Ridge Jennifer , Record Sydne , Rodrigues Jorge Luiz Mazza , Rodriguez-Reillo William , Shek Katherine L. , Takacs-Vesbach Tina , Blanchard Jeffrey L. 
Published: August 2024

AbstractSoil microbiomes are heterogeneous, complex microbial communities. Metagenomic analysis is generating vast amounts of data, creating immense challenges in sequence assembly and analysis. Although advances in technology have resulted in the ability to easily collect large amounts of sequence data, soil samples containing thousands of unique taxa are often poorly characterized. These challenges reduce the usefulness of genome-resolved metagenomic (GRM) analysis seen in other fields of microbiology, such as the creation of high quality metagenomic assembled genomes and the adoption of genome scale modeling approaches. The absence of these resources restricts the scale of future research, limiting hypothesis generation and the predictive modeling of microbial communities. Creating publicly available databases of soil MAGs, similar to databases produced for other microbiomes, has the potential to transform scientific insights about soil microbiomes without requiring the computational resources and domain expertise for assembly and binning.
Beneficial effects of intermittent fasting on nonalcoholic fatty liver disease: a narrative review
Authors: Deng Qingxuan , Lv Ruyue , Zou Haoqi , Zou Tangbin 
Published: August 2024

AbstractNonalcoholic fatty liver disease (NAFLD) is one of the most common chronic liver diseases, and it is characterized by a series of fatty liver diseases that can lead to severe liver disease. Although no therapeutic drug has been approved as an effective therapy for NAFLD to date, dietary changes and physical activity are thought to be the cornerstone of NAFLD management. For this reason, some articles are available to analyze the studies done so far using various modifications of intermittent fasting (IF) among animals and patients with NAFLD. Data from preclinical and clinical trials suggested that IF positively impacts inflammatory and metabolic markers in both animals and humans. Inflammation and oxidative stress are the major risk factors involved in the pathogenesis of NAFLD. IF has been shown to have positive benefits in alleviating metabolic disorders, promoting the browning of white tissue, resetting circadian rhythm, and activating autophagy of cells. This review is intended to provide a detailed synopsis of the protocols, potential mechanisms of action, and supporting evidence for IF in NAFLD. We will highlight what is currently known about IF approaches in NAFLD treatments in clinical populations with mechanism insight from animal studies, and the safety concerns in certain patient groups.
Graphical Abstract
The protocols of intermittent fasting (IF) are various. Data from trials suggested that IF positively impacts both humans and animals. IF has been shown to have potential treatments for nonalcoholic fatty liver disease.
Cureit: An End-to-End Pipeline for Implementing Mixture Cure Models With an Application to Liposarcoma Data
Authors: Whiting Karissa , Fei Teng , Singer Samuel , Qin Li-Xuan 
Published: August 2024

PURPOSE Cure models are a useful alternative to Cox proportional hazards models in oncology studies when there is a subpopulation of patients who will not experience the event of interest. Although software is available to fit cure models, there are limited tools to evaluate, report, and visualize model results. This article introduces the cureit R package, an end-to-end pipeline for building mixture cure models, and demonstrates its use in a data set of patients with primary extremity and truncal liposarcoma. METHODS To assess associations between liposarcoma histologic subtypes and disease-specific death (DSD) in patients treated at Memorial Sloan Kettering Cancer Center between July 1982 and September 2017, mixture cure models were fit and evaluated using the cureit package. Liposarcoma histologic subtypes were defined as well-differentiated, dedifferentiated, myxoid, round cell, and pleomorphic. RESULTS All other analyzed liposarcoma histologic subtypes were significantly associated with higher DSD in cure models compared with well-differentiated. In multivariable models, myxoid (odds ratio [OR], 6.25 [95% CI, 1.32 to 29.6]) and round cell (OR, 16.2 [95% CI, 2.80 to 93.2]) liposarcoma had higher incidences of DSD compared with well-differentiated patients. By contrast, dedifferentiated liposarcoma was associated with the latency of DSD (hazard ratio, 10.6 [95% CI, 1.48 to 75.9]). Pleomorphic liposarcomas had significantly higher risk in both incidence and the latency of DSD ( P < .0001). Brier scores indicated comparable predictive accuracy between cure and Cox models. CONCLUSION We developed the cureit pipeline to fit and evaluate mixture cure models and demonstrated its clinical utility in the liposarcoma disease setting, shedding insights on the subtype-specific associations with incidence and/or latency.
Durability of Response With Selpercatinib in Patients With RET-Activated Thyroid Cancer: Long-Term Safety and Efficacy From LIBRETTO-001
Authors: Wirth Lori J. , Brose Marcia S. , Subbiah Vivek , Worden Francis , Solomon Ben , Robinson Bruce , Hadoux Julien , Tomasini Pascale , Weiler Daniela , Deschler-Baier Barbara , Tan Daniel S.W. , Maeda Patricia , Lin Yan , Singh Ravinder , Bayt Theresa , Drilon Alexander , Cassier Philippe A. 
Published: August 2024

Clinical trials frequently include multiple end points that mature at different times. The initial report, typically based on the primary end point, may be published when key planned co-primary or secondary analyses are not yet available. Clinical Trial Updates provide an opportunity to disseminate additional results from studies, published in JCO or elsewhere, for which the primary end point has already been reported. LIBRETTO-001 is a registrational phase I/II, single-arm, open-label study of selpercatinib in patients with RET (REarranged during Transfection)-activated cancers (ClinicalTrials.gov identifier: NCT03157128 ). We present long-term safety and efficacy from LIBRETTO-001 in patients with RET-mutant medullary thyroid cancer (MTC; n = 324) and RET fusion-positive thyroid cancer encompassing different histological subtypes (TC; n = 66). At the data cutoff of January 2023, the objective response rate was 82.5% among patients with cabozantinib/vandetanib-naïve MTC and 95.8% among patients with treatment-naïve TC. At a median follow-up time of 42.4 and 44.0 months in patients with cabozantinib/vandetanib-naïve and pretreated MTC, the median progression-free survival (PFS) was not reached and 41.4 months, respectively. At a median follow-up time of 24.9 and 30.4 months in patients with treatment-naïve and pretreated TC, the median PFS was not reached and 27.4 months, respectively. Three-year PFS rates were 75.2% and 87.3% among patients with cabozantinib/vandetanib-naïve MTC and treatment-naïve TC, respectively. Median PFS was similar to median duration of response for each patient group. The safety profile of selpercatinib was consistent with previous reports. With an additional follow-up of 37 months and 228 more patients from the last disclosure, selpercatinib continued to provide durable and robust responses in treatment-naïve and previously treated patients with RET-mutant MTC and RET fusion-positive TC.
Perspective on physical interpretations of Rényi entropy in statistical mechanics
Authors: Ozawa Misaki , Javerzat Nina 
Published: August 2024

Abstract
Rényi entropy is a one-parameter generalization of Shannon entropy, which has been used in various fields of physics. Despite its wide applicability, the physical interpretations of the Rényi entropy are not widely known. In this paper, we discuss some basic properties of the Rényi entropy relevant to physics, in particular statistical mechanics, and its physical interpretations using free energy, replicas, work, and large deviation.
Contribution of Coordination Theories to the Determination of Human Factors Associated With Operating Room Perceived Performance
Authors: Dahmani Souhayl , Waelli Mathias , Dariel Odessa 
Published: August 2024

BACKGROUND:
The efficient and fluid organization of surgical interventions in an operating room (OR) and operating suite (OS) is important as these are among the most expensive units to run in medical-surgical facilities. The complexity of OS organization requires careful coordination, defined here as the directing of individuals’ efforts toward achieving common and explicitly recognized goals. There is currently sparse literature on OS coordination, especially in the French context. This study aimed to respond to this gap by reporting on the coordination mechanisms associated with the perceived performance of OS across 4 facilities in an urban setting in France.

METHODS:
We used a qualitative comparative case study based on ethnographic methodology to explore 4 facilities (2 teaching, 1 general, and 1 private). Several investigation techniques were used for data collection (semistructured interviews, participant and nonparticipant observations, and informal interviews) in the OR, the OS, the regulation council (dedicated to adapting the necessary resources to specific procedures and patients’ health status), and the OS council (dedicated to strategic and operational OS transformations and adaptations, and responsible for finding solutions to organizational problems). Analysis was guided by Okhuysen and Bachky’s theoretical framework on coordination and multi-team systems theory. Data were compared across the 4 facilities and triangulated using the different techniques to ensure coherence and accuracy.

RESULTS:
Overall, 48 interviews with health care providers and hospital managers and 200 hours of direct observations were performed. The OR exhibited a high degree of coordination, whereas improved perception of performance in the OS depended on managerial competency, trust, and authority. Perceived performance in the regulation council and OS council, on the other hand, depended on the identification of formal objectives by all stakeholders and the development of common understanding (developing agreement, direct information sharing, creating common perspective, substitution, bringing groups together, and storing of knowledge).

CONCLUSIONS:
Based on existing literature on multi-team systems (as represented in the OS organization), this study identifies success factors influencing OS coordination. These include the OS manager’s leadership skills; the identification of formal system objectives; and professional differentiation between stakeholders (absence/decrease of a sense of belonging to a multi-team system). This differentiation was related to the high degree of specialization within OS teams, each bringing different norms, cultures, and contingencies that induce dissonance in organization and task performance. Interventions targeting these success factors might improve coordination, and thus performance, in the OS.
Does Nociception Level Index-Guided Opioid Administration Reduce Intraoperative Opioid Consumption? A Systematic Review and Meta-Analysis
Authors: Sogut Muhammet Selman , Kalyoncu Ilayda , Karakaya Muhammet Ahmet , Manici Mete , Darçin Kamil 
Published: August 2024

BACKGROUND:
The nociception level (NOL) index is a quantitative parameter derived from physiological signals to measure intraoperative nociception. The aim of this systematic review and meta-analysis was to evaluate if NOL monitoring reduces intraoperative opioid use compared to conventional therapy (opioid administered at clinician discretion).

METHODS:
This meta-analysis comprises randomized clinical trials comparing NOL-guided opioid administration to conventional therapy in adult patients undergoing any type of surgery. A systematic search of PubMed, Scopus, and CENTRAL databases was conducted. The primary outcome was intraoperative opioid consumption and the effect estimate of the NOL index was measured using the standardized mean difference (SMD) where 0.20 is considered a small and 0.80 a large effect size. A random-effects model with Hartung-Knapp-Sidik-Jonkman adjustment was applied to estimate the treatment effect. Heterogeneity was explored clinically and statistically (using the inconsistency I² statistic, prediction intervals, and influence analysis). The quality (certainty) of evidence was evaluated using the Grading of Recommendations, Assessment, Development, and Evaluations (GRADE) guidelines methodology.

RESULTS:
This review comprised 9 trials (519 patients). The intraoperative opioid SMD (NOL monitoring versus conventional therapy) was −0.26 (95% confidence interval [CI], −0.82 to 0.30; P = .31; low certainty of evidence). We observed substantial clinical (intraoperative opioid regimens) and statistical heterogeneity with the I² statistic being 86% (95% CI, 75%–92%). The prediction interval was between −1.95 and 1.42 indicating where the SMD between NOL and conventional therapy would lie if a similar study were conducted in the future.

CONCLUSIONS:
This meta-analysis does not provide evidence supporting the role of NOL monitoring in reducing intraoperative opioid consumption.
Deprivation links to bystander cardiopulmonary resuscitation and defibrillation rates
Authors: Kate Keeping Chloe , Adam Whitley Gregory 
Published: August 2024

Background: Public access defibrillators and cardiopulmonary resuscitation (CPR) have been at the forefront of public health campaigns and public education and are key links in the chain of survival. Despite this, survival rates for out-of-hospital cardiac arrest (OHCA) in 2024 in the UK are lower than in comparable countries. Aims: This study aimed to critically review research in the UK to identify whether a patient's level of socioeconomic deprivation impacts their chances of receiving bystander CPR and defibrillation. Methods: A rapid evidence review was conducted with literature searches from 2013 to July 2023 carried out. Five studies were identified. Findings: OHCA incidence was lowest in areas of least deprivation, while the most deprived populations had lower rates of bystander CPR (BCPR) with higher OHCA incidences. Automated external defibrillator (AED) provision did not correlate with OHCA incidence. Conclusion: Research is needed to look at areas with a high incidence of OHCA associated with low rates of BCPR, with the aim to target high-risk areas of low BCPR and high OHCA incidences for public education and AED installation, including qualitative research into the population's thoughts and knowledge of CPR and defibrillation.