bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rare Atypical Intrapericardial Prostate Adenocarcinoma Metastasis With 68Ga-PSMA-11 PET/CT
Authors: Moreau Aurélie , Tabutin Mayeul , Bouhamama Amine , Kryza David 
Published: July 2024

Abstract
We report the case of an 88-year-old man recently diagnosed with prostate cancer. The patient underwent a 68Ga–prostate-specific membrane antigen–11 PET/CT for staging assessment. This examination revealed intense and expected uptake in the primary prostate cancer, widespread metastatic involvement including typical adenopathy and bone metastasis, and a less common pulmonary lymphangitic carcinomatosis. Most notably, we discovered a rare intrapericardial metastasis, which is an atypical site for metastasis in general and particularly for prostate adenocarcinoma.
An Evaluation of the Presence of Diversity, Equity, and Inclusion Information on Plastic Surgery Residency Program Websites
Authors: Cummings Olivia , Iheanacho Franklin , Gibson Jasmine , Araia Ermias , Ademuwagun Lydia , Charvis Jodi Sutherland , Tanzer Joshua , McIntire Damon , Kalliainen Loree 
Published: July 2024

Background
Increasing the diversity of plastic surgery trainees is an important step in providing optimal care for our increasingly diverse patient populations. Given that information presented on residency programs' websites can strongly influence applicants' decisions to apply to or rank a program, demonstrating a commitment to diversity, equity, and inclusion (DEI) on program websites may aid in recruiting applicants with URM background.

Methods
Using 8 DEI-related criteria, we evaluated the websites of 103 plastic surgery residency programs for the presence of DEI-related content during the month of June 2022. Each program was evaluated by 2 individual graders. We analyzed the data with confirmatory factor analysis in R using the Lavaan package.

Results
On average, programs fulfilled 2.1 ± 1.6 of the metrics with a range of 0–7 fulfilled per program. Our model revealed that the criteria were a high-quality (P < 0.0001) measure of DEI-related metrics. There was a significant association between program size and presence of DEI-related criteria (linear 0.039; quadratic −0.005; both P < 0.01), such that mid-sized programs (16–18 residents) had the highest quality of DEI advertising compared to both small and large programs. Programs associated with a USNWR Top 20 Hospital were less likely to achieve high level of DEI-related criteria than other programs (P < 0.0001).

Conclusion
Mid-sized programs had greater DEI quality on their websites, while smaller and larger programs similarly had poorer assessed quality. There is room for all programs to improve the presence of DEI-related material on their websites, especially related to care of transgender populations.
Machine learning nominal max oxygen consumption from wearable reflective pulse oximetry with density functional theory
Authors: Al Dajani Saleem Abdul Fattah Ahmed , Laquai Frédéric 
Published: July 2024

AbstractWearable sensors have revolutionized modern health towards precision medicine. Many measurements taken by wearables are not currently used for medical purposes, and their physical roots are not understood. Here we show the origin of inferring oxygen saturation from measuring estimated oxygen variation while preparing for a marathon via spectrophotometric pulsatile blood flow transcutaneous reflectance oximetry, building six years of data tracking the stages of running a marathon. Based on inputs from quantum mechanical simulations into classical wave and electromagnetic theory models, the imaginary part of the complex dielectric function of the active site of human hemoglobin is altered upon oxygen binding to the heme group, changing the extinction coefficient and selectively absorbing wavelengths of light in a way that enables its detection from the ratio of red to infrared absorbance. A fundamental nominal max oxygen consumption is inferred from quantum mechanical absorbance spectra with and without oxygen binding by training a machine learning model on 1.5 years of daily wearable reflective pulse oximetry data with an R2of 0.84. Reflectance oximetry is strongly correlated (R2of 0.96) to standard pulse oximetry, such as through earlobe or fingertip, enabling accurate, rapid, regular, and automated monitoring of vital signs with wearable sensors on smart watches and fitness trackers, and supplying artificial intelligence inferences of function-symptom relationships for precision medicine.
Dachsous is a key player in epithelial wound closure by modulating cell shape changes and cytoskeleton dynamics
Authors: Porfírio-Rodrigues Patrícia , Pereira Telmo , Jacinto Antonio , Carvalho Lara 
Published: July 2024

AbstractEpithelia are vital tissues in multicellular organisms, acting as barriers between external and internal environments. Simple epithelia, such as those in embryos and the adult gut, have the remarkable ability to repair wounds efficiently, making them ideal for studying epithelial repair mechanisms. In these tissues, wound closure involves the coordinated action of a contractile actomyosin cable at the wound edge and collective cell movements around the wound. However, the dynamics of cell-cell interactions during this process remain poorly understood.Here, we demonstrate that Dachsous (Ds), an atypical cadherin associated with Planar Cell Polarity, is crucial for efficient epithelial repair in theDrosophilaembryonic epidermis. We show that the absence of Ds leads to delayed wound closure, impaired actomyosin cable formation, and altered cell shape changes. Additionally, we reveal that Occluding Junctions are necessary for the proper apical localization of Ds, suggesting an unanticipated interaction between these two molecular complexes. This study identifies Ds as a novel player in epithelial repair and highlights the need for further investigating the molecular mechanisms by which Ds modulates cell shape and tissue morphogenesis.Summary statementThis study shows that the atypical cadherin Dachsous is essential for epithelial wound closure, influencing cytoskeletal dynamics and cell morphology, with Occluding Junctions regulating its subcellular localization.
PPR767 affects plant architecture and drought resistance by modulating complex I activity and ROS content in rice
Authors: Peng Leilei , Xiao Haijun , Xu Yanghong , Huang Zhihao , Yang Xuan , Lv Chen , Huang Linghui , Hu Jun 
Published: July 2024

AbstractThe RNA-binding proteins (RBPs) encoded by nucleus are essential for the metabolism of RNAs in eukaryotes. The pentatricopeptide repeat (PPR) proteins, a large subset of RBPs, participate in organellar RNA processing for plant development and reproduction. Here, we identified an E-type PPR protein, PPR767, which functions in mitochondria. Knocking outPPR767resulted in shorter plant height, thinner stems, shorter and narrower blades, and consequently affected yield traits, compared to those of the wild type. PPR767 primarily participated in the editing of 4 sites, nad1-674,nad3-155,nad3-172, andnad7-317. And PPR767 interplayed with MORF1 and MORF8, suggesting the editosome in rice is complicated. Meanwhile, the activity of mitochondrial complex I was decreased, and the structure of mitochondria was compromised in the mutants. Furthermore, mutation ofPPR767influenced rice drought tolerance and the expression levels of genes involved in the reactive oxygen species (ROS) accumulation. These findings suggest thatPPR767guarantees the complex I activity by properly regulating the RNA editing efficiency of mitochondrial genes and affects drought tolerance by modulating ROS content in rice, providing valuable insights into the mechanisms by which PPRs fulfil their functions.
Identifying essential genes inSchaalia odontolyticausing a highly-saturated transposon library
Authors: Bedree Joseph K. , Bourgeois Jacob , Balani Pooja , Cen Lujia , Hendrickson Erik L. , Kerns Kristopher A. , Camilli Andrew , McLean Jeffrey S. , Shi Wenyuan , He Xuesong 
Published: July 2024

AbstractThe unique epibiotic-parasitic relationship betweenNanosynbacter lyticustype strain TM7x, a member of the newly identified Candidate Phyla Radiation, now referred to asPatescibacteria, and its basibiont,Schaalia odontolyticastrain XH001 (formerlyActinomyces odontolyticus), require more powerful genetic tools for deeper understanding of the genetic underpinnings that mediate their obligate relationship. Previous studies have mainly characterized the genomic landscape of XH001 during or post TM7x infection through comparative genomic or transcriptomic analyses followed by phenotypic analysis. Comprehensive genetic dissection of the pair is currently cumbersome due to the lack of robust genetic tools in TM7x. However, basic genetic tools are available for XH001 and this study expands the current genetic toolset by developing high-throughput transposon insertion sequencing (Tn-seq). Tn-seq was employed to screen for essential genes in XH001 under laboratory conditions. A highly saturated Tn-seq library was generated with nearly 660,000 unique insertion mutations, averaging one insertion every 2-3 nucleotides. 203 genes, 10.5% of the XH001 genome, were identified as putatively essential.
Frameshift variants inTBX2underlie autosomal-dominant hearing loss with incomplete penetrance of nystagmus
Authors: Hua Wan , Wang Yanfei , Li Xiang , Wang Lanchen , Xiong Wenyu , Chen Meilin , Bu Fengxiao , Liu Libo , Zhong Mingjun , Lu Yu , Liu Zhiyong , Cheng Jing , Yuan Huijun 
Published: July 2024

AbstractPurposeThe transcription factor TBX2 plays a critical role in inner hair cells development in mice. Yet, the link betweenTBX2malfunction and human hearing-related disorders remains unexplored.MethodsLinkage analysis combined with whole genome sequencing was applied to identify the causative gene in two autosomal dominant Chinese families characterized by late-onset progressive sensorineural hearing loss and incomplete penetrance of horizontal oscillatory nystagmus. Functional evaluation ofTBX2variants was performed through protein expression, localization, and transcriptional activity analysisin vitro, phenotypic analysis and mechanism study in knockout mice modelin vivo.ResultsMultipoint parametric linkage analysis of Family 1 revealed a maximum LOD score of 3.01 on chromosome 17q23.2. Whole genome sequencing identified distinctTBX2variants, c.977delA (p.Asp326Alafs*42) and c.987delC (p.Ala330Argfs*38) in each family, co-segregating with hearing loss. These variants resulted in premature termination and the generation of a new peptide segment, reducing transcriptional activity. Further, heterozygousTbx2knockout mice exhibited late-onset progressive hearing loss, along with ectopic expression of Prestin in IHCs and a gradual decrease in expression from P7 to P42.ConclusionOur findings indicate that heterozygousTBX2frameshift variants are the genetic cause of late-onset progressive hearing loss and incomplete penetrance of nystagmus. The heterozygousTbx2knockout mouse model mirrored the human hearing loss phenotype, further validating TBX2’s role in auditory function. These insights enhance our understanding ofTBX2in the auditory system, providing valuable information for molecular diagnostics and genetic counseling in related hearing disorders.
Characterizing the Spatial Distribution of Dendritic RNA at Single Molecule Resolution
Authors: Kim Jihoon , Rosario Jean G. , Mendoza Eric , Kuang Da , Kim Junhyong 
Published: July 2024

ABSTRACTNeurons possess highly polarized morphology that require intricate molecular organization, partly facilitated by RNA localization. By localizing specific mRNA, neurons can modulate synaptic features through local translation and subsequent modification of protein concentrations in response to stimuli. The resulting activity-dependent modifications are essential for synaptic plasticity, and consequently, fundamental for learning and memory. Consequently, high-resolution characterization of the spatial distribution of dendritic transcripts and the spatial relationship across transcripts is critical for understanding the pathways and mechanisms underlying synaptic plasticity. In this study, we characterize the spatial distribution of six previously uncharacterized genes (Adap2,Colec12,Dtx3L,Kif5c,Nsmf,Pde2a) within the dendrites at a sub-micrometer scale, using single-molecule fluorescence in situ hybridization (smFISH). We found that spatial distributions of dendritically localized mRNA depended on both dendrite morphology and gene identity that cannot be recreated by diffusion alone, suggesting involvement of active mechanisms. Furthermore, our analysis reveals that dendritically localized mRNAs are likely co-transported and organized into clusters at larger spatial scales, indicating a more complex organization of mRNA within dendrites.
FASTKD5 processes mitochondrial pre-mRNAs at non-canonical cleavage sites
Authors: Antonicka Hana , Weraarpachai Woranontee , Vučković Ana , Hillen Hauke S. , Shoubridge Eric A. 
Published: July 2024

AbstractThe regulation of mammalian mitochondrial gene expression is largely post-transcriptional and the first step in translating the 13 polypeptides encoded in mtDNA is endonucleolytic cleavage of the primary polycistronic transcripts. As the rRNAs and most of the mRNAs in mtDNA are flanked by tRNAs, the release of the mature RNAs occurs mostly by excision of the tRNAs. Processing the non-canonical mRNAs, not flanked by tRNAs, requires FASTKD5, but the molecular mechanism remains unknown. To investigate this, we created and characterized a knockout cell line to use as an assay system. The absence of FASTKD5 resulted in a severe combined OXPHOS assembly defect due to the inability to translate mRNAs with unprocessed 5’-UTRs. Analysis of RNA processing of FASTKD5 variants allowed us to map amino acid residues essential for function. Remarkably, this map was RNA substrate-specific, arguing against a one size fits all model. A reconstitutedin vitrosystem with purified FASTKD5 protein and synthetic RNA substrates showed that FASTKD5 on its own was able to cleave client substrates correctly, but not non-specific RNA sequences. These results establish FASTKD5 as the missing piece of the biochemical machinery required to completely process the primary mitochondrial transcript.
Sodium butyrate is incorporated into central metabolism in fly head while inducing oxygen consumption increase
Authors: Müller-Eigner Annika , Gille Benedikt , Dethloff Frederik , Meng Chen , Ludwig Christina , Heiker John T , Giavalisco Patrick , Peleg Shahaf 
Published: July 2024

AbstractButyrate has been proposed as a drug therapy by acting as a KDAC inhibitor and elevating protein acetylation, in particular on histones. Nonetheless, recent studies suggest that tissues such as the gut can utilize butyrate as a metabolite. We have previously shown that the addition of butyrate induces a rapid increase of oxygen consumption in wholeDrosophila melanogasterheads. Here we show that while head oxygen consumption is increased by the addition of butyrate, no apparent changes are observed on the proteome and acetylome. Instead, we show that butyrate is metabolized and incorporated into the tricarboxylic acid cycle (TCA) cycle. Collectivity our data supports the notion that the therapeutic benefits of acute butyrate treatment may be also mediated by improving metabolic rates, rather than solely targeting the epigenome or acetylome.
Stabilized dengue virus 2 envelope subunit vaccine redirects the neutralizing antibody response to all E-domains
Authors: Thiono Devina J. , Samaras Demetrios , Phan Thanh T.N. , Zhu Deanna R. , Shah Ruby P. , Castillo Izabella , Forsberg Lawrence J. , Premkumar Lakshmanane , Baric Ralph S. , Tian Shaomin , Kuhlman Brian , de Silva Aravinda M. 
Published: July 2024

AbstractThe four-dengue virus (DENV) serotypes cause several hundred million infections annually. Several live-attenuated tetravalent dengue vaccines (LAVs) are at different stages of clinical testing and regulatory approval. A major hurdle faced by the two leading LAVs is uneven replication of vaccine serotypes stimulating a dominant response to one serotype at the expense of the other three, leading to the potential for vaccine antibody (Ab) enhanced more severe infections by wild type DENV serotypes that fail to replicate in the vaccine. Protein subunit vaccines are a promising alternative since antigen dosing can be precisely controlled. However, DENV envelope (E) protein subunit vaccines have not performed well to date, possibly due to differences between the monomeric structure of soluble E and the E homodimer of the viral surface. Previously, we have combined structure-guided computational and experimental approaches to design and produce DENV2 E antigens that are stable homodimers at 37℃ and stimulate higher levels of neutralizing Abs (NAbs) than the WT E antigen in mice. The goal of this study was to evaluate if DENV2 E homodimers stimulate NAbs that target different epitopes on E protein compared to the WT E monomer. Using DENV4/2 chimeric viruses and Ab depletion methods, we mapped the WT E-elicited NAbs to simple epitopes on domain III of E. In contrast, the stable E homodimer stimulated a more complex response towards all three surface-exposed domains of the E protein. Our findings highlight the impact of DENV2 E oligomeric state on the quality and specificity of DENV NAbs, and the promise of DENV E homodimers as subunit vaccines.ImportanceThe ideal dengue virus (DENV) vaccine should elicit a balanced and highly protective immune response against all 4 DENV serotypes. Current tetravalent live-attenuated DENV vaccines have faced challenges due to uneven replication of vaccine virus strains stimulating a strong immune response to one serotype and weak responses to the other three. Protein subunit vaccines provide novel opportunities to stimulate a balanced response because dosing can be precisely controlled and independent of vaccine virus replication. Here, we compare immune responses elicited by a new DENV serotype 2 protein vaccine designed to match the structure of proteins on the viral surface. We find that proteins designed to match the viral surface stimulate better immune responses targeting multiple sites on the viral surface compared to previous protein vaccines. Our results justify further testing and development of these second-generation DENV protein subunit vaccines.
Incidence and multidrug resistance ofEscherichia colipathotypes on fresh vegetables and salads
Authors: Vázquez-Quiñones C. R. , Rincón-Guevara M.A , Natividad-Bonifacio I. , Vázquez-Salinas C. , González-Márquez H. 
Published: July 2024

1AbstractDiarrhea is a severe health problem and one of the leading causes of child mortality in Africa, Asia, and Latin America. Diarrhea is often caused by consuming contaminated food and improperly washed fruit and vegetables that harbor norovirus,Campylobacter, non-typhoidSalmonella, and pathogenicEscherichia coli.The research examined 334 samples of ready-made salads with lettuce, carrots, tomatoes and fresh coriander and lettuce. Genotyping involved detectingstandltgenes for enterotoxigenicE. coli(ETEC) and theuidA gene for beta-glucuronidase. ETEC was found in 51.56% of confirmed isolates, accounting for 9.9% of samples gathered in two years. Contamination rates by ETEC were 78.78% in coriander, 9.09% in lettuce, 9.09%, and 3.03% in green salads from La Vicentina and La Purísima markets, respectively. Among samples, 12.12% have both fragments (st/lt), 33.3% have only theltfragment, and 54.6% have justst. In salads, thelt/stgenes were detected in 9.09% (3),ltin 3.3% (1), andstwas absent. In coriander, 21.21% havelt, 51.51%st, and 6.06% showed amplification for both. ETEC was found in 9.09% of the confirmed lettuce strains, with 3.03%ltgene, 3.03%stgene, and 3.03% both (lt/st). There are no reported data with the same ratios for Mexico City. ETEC’s presence in products consumed in markets or streets in Mexico City, coupled with lifestyle changes that have increased vegetable consumption, is a significant concern. These findings underscore the potential health implications and the urgent need for further investigation and preventive measures.
Women on board, institutional ownership and emissions disclosure practices as tools for attaining sustainable development goals in Nigeria
Authors: Jibril Rabiu Saminu 
Published: July 2024

Purpose
This study aims to examine how women on board influence quality and quantity disclosure of emissions discharge by the listed non-financial firms for the period of six years (2016–2021), with institutional ownership as a moderator.

Design/methodology/approach
The study obtained data from a sample of 83 listed non-financial firms. A content analysis technique was employed to compute emissions disclosure indexes using Global Reporting Initiatives standards from the sampled firms. Random and fixed effect regression analyses were run for both direct and moderation models. Based on the results of the Hausman tests, random results were adopted and used in examining the relationship.

Findings
The result reveals that women on board are significantly related to emission disclosure. The study also documented that institutional owners have not influenced the relationship between women directors and emissions disclosure.

Practical implications
The study's findings have practical implications for emerging economies, corporations and other business organizations seeking to actively involve the emissions control and reduction issues toward sustainable development goals 5, 7 and 13 in their business models and successfully communicate these efforts to stakeholders.

Social implications
Listed firms in emerging economies would gain sincerity through the women directors’ knowledge, skills, demographics and ethnicity in the society. Therefore, corporate bodies in emerging economies can successfully contribute toward improving the social welfare of various segments of society by controlling current and future climate issues. Additionally, society will surely benefit when firms control the pollution discharges within the community.

Originality/value
This is the first study, to the best of the authors’ knowledge, that provides empirical evidence on the effect of the presence of women on board on emissions disclosure using institutional ownership as a moderator in Nigeria.
Ethics in quantitative sport management research: the impact of AI
Authors: Trail Galen , Kim Ari , Bang Hyejin , Braunstein-Minkove Jessica R. 
Published: July 2024

PurposeDespite the use of plagiarism-checking software and current ethical guidelines in sport management journals, raising awareness of ethical concerns and potential risks of artificial intelligence (AI) applications is necessary. This paper discusses how AI affects ethical research and publishing and provides guidelines for sport management scholars to ensure quality and integrity of their research.Design/methodology/approachA comprehensive review and critical analysis of literature was performed to evaluate research ethics, potential risks, and guiding principles for the use of AI in research.FindingsEthical research guidelines for quantitative sport management research were proposed. The guidelines encompass seven principles for the proper use of AI and ethical conduct specific to the research methods, data analysis, and results, which would be challenging for AI to accurately replicate.Originality/valueThis study provides an original contribution to the field of sport management because numerous questions concerning ethics and AI have not been addressed until now. The guidelines are suitable for use by sport management scholars, concerning the accuracy, validity, and quality of research while mitigating ethical risks in AI-generated content.