bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Operation of Automobile Engines on Biofuel E85
Authors: Polcar Adam , Skřivánek Antonín , Čupera Jiří 
Published: February 2013

Shrnutí
Článek se zabývá monitorováním výstupních parametrů automobilových motorů při provozování na biopalivo E85 s Pro analýzu byla vybrána dvě vozidla a jeden motor. Měření vozidel bylo provedeno pomocí válcového dynamometru na vozidlové zkušebně. Motor byl testován na zkušebně motorů pomocí vířivého dynamometru. Jako referenční palivo byl zvolen benzín Natural 95 (BA95). Vozidla byla pro spalování E85 vybavena dodatečnými přestavbovými sadami. Seřízení motoru proběhlo pomocí programovatelné řídicí jednotky. Všechna měření probíhala při ustálených parametrech. Mezi hodnocené parametry patřila spotřeba motoru, výkon a efektivní účinnost. Výsledky měření poukazují na pozitivní vliv etanolu na spalovací proces u všech hodnocených motorů. Jak je z výsledku dále patrné má majoritní vliv na změnu výstupních parametrů i seřízení motoru resp. vstřikované dávky
Energy absorption by ferromagnetic nanoparticles in hyperthermia therapy
Authors: Kurgan Eugeniusz 
Published: February 2013

Abstract
A numerical method is developed for estimation of temperature distributions inside tissues heated by external RF hyperthermia with external circular coil. The computational method relies on a solution of electromagnetic field problem in sinusoidal steady state. The heat transfer problem is treated in three dimensions with axis symmetry model. Than the bioheat diffusion equation under a steady-state condition is solved to determine the temperature distributions inside tumour and surrounding tissues. The heat removal due to the blood circulation is also taken into account. Numerical results are presented for heat generated by ferromagnetic nanoparticles in order to minimize negative effects of radiofrequency radiation.
Streamflow Changes in Mesoscale Lowland Catchment Under Future Climate Conditions
Authors: Somorowska Urszula , Piętka Izabela 
Published: February 2013

AbstractThe objective of this study was to investigate the performance of streamflow in a lowland mesoscale catchment in Poland under current and future climate conditions. Simulations of hypothetical streamflow in the future climate were facilitated by meteorological data sets from ensemble simulations from all over Europe with the Regional Climate Model CLM. Projections of precipitation and air temperature for the 21st century under the SRES A1B scenario were used as an input to the hydrological model simulating streamflow at the daily time scale. The combination of relatively moderate increase of annual precipitation sum and mean air temperature might cause lower annual discharges. The possible decrease in stream water resources might be a signal of reduced subsurface recharge and land over drying processes.
Geotechnical Parameters of Alluvial Soils from in-situ Tests
Authors: Młynarek Zbigniew , Stefaniak Katarzyna , Wierzbicki Jędrzej 
Published: February 2013

Abstract
The article concentrates on the identification of geotechnical parameters of alluvial soil represented by silts found near Poznan and Elblag. Strength and deformation parameters of the subsoil tested were identified by the CPTU (static penetration) and SDMT (dilatometric) methods, as well as by the vane test (VT). Geotechnical parameters of the subsoil were analysed with a view to using the soil as an earth construction material and as a foundation for buildings constructed on the grounds tested. The article includes an analysis of the overconsolidation process of the soil tested and a formula for the identification of the overconsolidation ratio OCR. Equation 9 reflects the relation between the undrained shear strength and plasticity of the silts analyzed and the OCR value. The analysis resulted in the determination of the Nkt coefficient, which might be used to identify the undrained shear strength of both sediments tested. On the basis of a detailed analysis of changes in terms of the constrained oedometric modulus M0, the relations between the said modulus, the liquidity index and the OCR value were identified. Mayne’s formula (1995) was used to determine the M0 modulus from the CPTU test. The usefullness of the sediments found near Poznan as an earth construction material was analysed after their structure had been destroyed and compacted with a Proctor apparatus. In cases of samples characterised by different water content and soil particle density, the analysis of changes in terms of cohesion and the internal friction angle proved that these parameters are influenced by the soil phase composition (Fig. 18 and 19). On the basis of the tests, it was concluded that the most desirable shear strength parameters are achieved when the silt is compacted below the optimum water content.
Polymorphism in The Region of Bovine Prion Protein Gene and Selected Haematological Indices in Cows Naturally Infected with Bovine Leukaemia Virus
Authors: Kaczmarczyk Ewa , Bojarojć-Nosowicz Barbara , Czarnik Urszula 
Published: February 2013

Abstract
The objective of this study was to determine whether insertion-deletion (indel) 23 bp polymorphism of the bovine prion protein (PRNP) gene differentiates the total number of leukocytes and lymphocytes and the number of virus-infected lymphocytes. The experimental materials comprised 119 Black-and-White Polish Holstein-Friesian cows. Bovine leukosis was diagnosed by an indirect immunofluorescence based on the detection of viral protein p24 in bovine lymphocytes infected with leukaemia virus (BLV). Indel23 polymorphism was determined by PCR. Blood haematological parameters (total leukocyte counts, total lymphocyte counts, and their percentages) were determined at a specialist haematological laboratory. The examined indices were analysed in three replications, at one-month intervals. It was found that indel23 polymorphism significantly differentiated blood leukocyte counts and the total number and percentage of lymphocytes. Cows with the 23 bp del/del genotype showed significantly higher leukocyte and lymphocyte counts than animals with the remaining two genotypes. Higher values of the analysed haematological parameters noted in homozygotes with 23 bp deletion are similar to the values reported in cows affected by persistent lymphocytosis, thus pointing to an adverse effect of this genotype on the haemopoiesis process. The variations between indel23 genotypes and the number and percentage of BLV-infected lymphocytes are less obvious and more difficult to interpret.
Pathological Features of Experimental Bovine Leukaemia Viral (BLV) Infection in Rats and Rabbits
Authors: Dimitrov Petar , Simeonov Konstantin , Todorova Katerina , Ivanova Zina , Toshkova Reneta , Shikova Evelina , Russev Russy 
Published: February 2013

Abstract
Rabbits and rats were inoculated with material derived from FLK cells producing permanently bovine leukaemia virus (BLV). The viral presence in the inoculum was proved by transmission electron microscopy, immunofluorescence, immunogold labelling demonstrating viral Tax protein, and PCR analysis. About 30 % of the infected animals sustained BLV seropositivity during the experiment, and demonstrated symptoms of lympholeukaemia - clinical manifestation of an immunosuppressive condition, increased number of lymphocytes and lymphoblasts, and preneoplastic lymphoid cell accumulations in the liver, lungs, kidneys, and lymph nodes. BLV DNA, detected by PCR in diseased animals, indicates the role of BLV as an aetiological factor of lympholeukaemia, developed in these animals after BLV infection. The alterations in rats were more pronounced than those in rabbits. The results prove that these two species of laboratory animals, especially rats, are suitable models for the in vivo studies of leukaemogenesis caused by BLV/HTLV infections.
Preliminary Study on the Safety of a New Vaccine Against Canine Babesiosis Containing Soluble Parasitic Antigen (SPA)
Authors: Adaszek Łukasz , Wernicka-Furmaga Romana , Winiarczyk Stanisław 
Published: February 2013

Abstract
The aim of this study was to assess the safety of a new vaccine, containing soluble parasitic antigen (SPA), against canine babesiosis. Fifteen dogs were included in the experiment. Five controls received only the adjuvant and 10 dogs were vaccinated with Babesia canis canis SPA twice, at a two weeks interval. For the whole period of the study all animals were under constant clinical observation. Haematological and biochemical tests were performed. Flu-like symptoms and local reactions at the injection site were observed in three animals from the vaccinated group and in two dogs from the control group. These events were transient, receded spontaneously and did not require any appropriate treatment. In 50% of the vaccinated dogs, a slight and spontaneously receding thrombocytopenia developed. However, in none of the animals used in the experiment shock symptoms were observed. Administration of the SPA did not affect the functions of internal organs, which was confirmed by normal results of biochemical tests. The obtained Babesia canis SPA can be considered safe and well tolerated by dogs, and therefore it can be used in further studies on the immunisation of animals against babesiosis.
Effect of a Protein-Xanthophyll Concentrate from Alfalfa and of Genotype and Sex of Lambs on their Blood Redox Profile
Authors: Ognik Katarzyna , Patkowski Krzysztof , Grela Eugeniusz R. 
Published: February 2013

Abstract
The experiment was conducted on lambs of the Polish Lowland sheep (PLS) and a synthetic BCP line. The redox potential was assessed in blood of lambs with different genotype and sex, which were fed feed mixtures with various contents of a proteinxanthophyll (PX) concentrate from alfalfa (1.5% and 3%). The 1.5% addition of PX concentrate to feed stimulated the response of the antioxidant defence mechanisms of the lambs to a greater extent than the 3% addition. The study showed that sex of the lambs had no significant effect on changes in the redox parameters of blood. The PLS genotype of lambs determined higher values of superoxide dismutase (SOD), total antioxidant potential of plasma (FRAP), malondialdehyde, and vitamin C than the BCP genotype. Along with the growth of the lambs, a descending tendency was observed in SOD and catalase levels, and an ascending tendency in FRAP value.
A Morphometric Analysis of the Geniculate Bodies in Selected Mammalian Species
Authors: Najdzion Janusz , Wasilewska Barbara , Bogus-Nowakowska Krystyna , Równiak Maciej , Żakowski Witold , Robak Anna 
Published: February 2013

Abstract
Unbiased stereological methods were used to examine and compare morphometrically the geniculate bodies (GB) in representatives of four mammalian orders (Insectivora, Rodentia, Lagomorpha, and Carnivora). The significant disproportion was observed between the relative sizes of both geniculate nuclei and their neuronal populations in the common shrew and the bank vole. The medial geniculate body (MGB) in the common shrew definitely surpassed the lateral geniculate body (LGB) in terms of percentage volume and percentage number of neurons. The volume of the GB and their nuclei correlated with their mean neuronal populations, whereas the negative correlation was observed between volumes and neuronal density; however, not as distinct as in the non-sensory brain structures. In all examined species, the LGB always had a higher numerical density than the MGB, while the MGB neurons were always distinctly larger than that of the LGB, which clearly differentiated both neuronal complexes. Analysis of these data shows that the GB differs in terms of the morphometric characteristics in the studied species.
Chlamydiales – Taxonomy, Pathogenicity, and Zoonotic Potential
Authors: Niemczuk Krzysztof , Truszczyńsk Marian , Szymańska-Czerwińska Monika 
Published: February 2013

Abstract
Changes in the taxonomy of the order Chlamydiales, after its separation from the order Rickettsiales, were presented. These changes resulted in the recognition of the following families: Chlamydiaceae, Chlavichlamydiaceae, Criblamydiaceae, Parachlamydiaceae, Piscichlamydiaceae, Rhabdochlamydiaceae, Simkaniaceae, and Waddliaceae. Other described changes concerned particularly the family Chlamydiaceae. Its genus Chlamydia was divided into Chlamydia and Chlamydophila. However, in the following years, a revision to the single original genus was made, based upon phylogenetic analysis of 16S and 23S rRNA genes of the strains belonging to these two taxonomic units. The review also discusses other families outside the family Chlamydiaceae, which contain so-called Chlamydia-related or Chlamydia-like organisms. Members of each family share a 16S rDNA gene sequence similarity >90%. Furthermore, characterisation of the pathogenecity is presented, focusing especially on the representatives of the family Chlamydiaceae, which cause animal infections, and describing their zoonotic potential. Available data on this topic, connected with the representatives of other families, were mentioned.
Monitoring of Selected Genes in Campylobacter Jejuni and Campylobacter Coli Isolates from Domestic Animals
Authors: Selwet Marek , Galbas Mariola 
Published: February 2013

Abstract
In the present study, the presence of flaA, cadF, cdtB, and iam genes of Campylobacter sp. were analysed using PCR. Material for analyses comprised 100 Campylobacter sp. isolates obtained from healthy broiler chickens, fatteners, and calves, among which 84 isolates were ascribed to Campylobacter jejeuni and 16 to Campylobacter coli. All isolates (100%) had the cadF gene responsible for adhesion and the flaA gene determining the motility of the analysed bacteria. The frequency of occurrence of the cdtB gene responsible for the production of the cytolethal distending toxin (CDT) was determined to be high (98.6% in broiler chickens, 75% in fatteners, 62.5% in calves). In case of the iam gene, the highest frequency was recorded in Campylobacter sp. isolated from broiler chickens (84.7%), while in strains collected from fatteners and calves it was lower, amounting to 41.7% and 18.8%, respectively.
Simultaneous Determinaton of Fluoroquinolones in Feed by Liquid Chromatography with Fluorescence Detection
Authors: Gbylik Małgorzata , Posyniak Andrzej , Gajda Anna 
Published: February 2013

Abstract
An HPLC method with fluorescence detection (HPLC-FLD) was developed for the simultaneous quantitative determination of enrofloxacin, ciprofloksacin, sarafloxacin, and difloxacin in feed. The samples were extracted with 0.25% formic acid in ultrasonic bath and purified by solid phase extraction (SPE) on Strata-X cartridges. The samples were analysed on Luna C8 liquid chromatography column with gradient programme by acetonitrile and 0.025 M phosphoric acid with 0.0025 M sodium 1- heptanesulfonate monohydrate. The method was successfully validated according to the requirements of the European Decision 2002/657/EC. Recoveries of the components from spiked feed samples ranged from 55%-70%. Repeatability was <7%. The method presented here proved to be efficient, rapid, and a selective approach for determination of fluoroquinolones in feed.
Value of Fluorescence Polarisation Assay in Comparison to Traditional Techniques in Diagnosis of Porcine Brucellosis
Authors: Weiner Marcin , Iwaniak Wojciech , Szulowski Krzysztof 
Published: February 2013

Abstract
The aim of this study was the evaluation of fluorescence polarisation assay (FPA) in the diagnosis of porcine brucellosis in comparison with Rose Bengal test (RBT), serum agglutination test (SAT), complement fixation test (CFT), 2-mercaptoethanol test, and ELISA. Eight hundred seventeen sera from pigs, including 612 sera from healthy animals, seven sera from Brucella suis bv2 culture positive animals, and 198 sera classified as false positive, originated from confirmatory investigations, were used. All sera from healthy animals, negative in RBT, SAT, CFT, and ELISA were also negative in FPA. All sera positive in serological examination, originated from Brucella infected animals, were also positive in FPA. Among sera classified as false positive almost half of the samples tested (49.49%) reacted positively in FPA. The examinations confirmed the usefulness of FPA in diagnosis of porcine brucellosis, but the method, like the other tests, does not allow resolving the problem of discrimination cross-reacting from specific antibodies.
Occurrence of Leptospirosis in Domestic Animals Reared on Exposed or Non-Exposed to Flood Areas of Eastern Poland
Authors: Wasiński Bernard , Sroka Jacek , Wójcik-Fatla Angelina , Zając Violetta , Cisak Ewa , Knap Józef P. , Sawczyn Anna , Dutkiewicz Jacek 
Published: February 2013

Abstract
Blood serum samples collected from randomly selected groups of 32 pigs and 41 cows reared in farms belonging to the rural community “A” located in eastern Poland and exposed to the Vistula river floods, and serum samples from groups of 41 pigs and 40 cows from farms belonging to the rural community “B” located also in eastern Poland but not in the area exposed to floods, were examined by the microscopic agglutination test for the presence of antibodies against 18 Leptospira serovars. The percentage of serum samples presenting positive results with at least one serovar were higher in pigs and cows from community “A” comparing to community “B” (34.4% vs. 4.9% and 26.8% vs. 15.0%, respectively). In the case of pigs, the difference was statistically significant (P=0.0015). The reactions with 12 Leptospira serovars (Australis, Bataviae, Bratislava, Canicola, Hardjo, Hebdomadis, Icterohaemorrhagiae, Pomona, Poi, Cynopteri, Grippotyphosa, Celledoni,), belonging to four species (L. interrogans, L.
borgpetersenii, L. kirschneri, L. weili) were found in the examined animals. In community “B”, six reactions with one serovar and two reactions with two serovars were noted whereas in community “A” - 19 reactions with one serovar, one reaction with two serovars and two reactions with six serovars were observed. The titres in animals reared in community “A” were significantly higher (up to 25,600) compared to community “B” (up to 200, P=0.0094). The obtained results suggest that the exposure to flooding may increase the infection rate in pigs and cows from afflicted areas to some extent.
Analysis of Hair Coat of Dachshund of Longhaired, Shorthaired, and Wirehaired Variety
Authors: Czyż Katarzyna , Patkowska-Sokoła Bożena , Filistowicz Andrzej , Janczak Marzena , Bodkowski Robert 
Published: February 2013

Abstract
The aim of the study was to analyse the hair coat of the three dachshund varieties, i.e. shorthaired, longhaired, and wirehaired, with respect to its histological structure and content of selected chemical elements. The study was conducted on female dachshunds originating from individual breeders. The study included the assessment of the contribution of particular hair fractions, measurements of hair thickness and length, and also the analysis of histological structure of particular hair fractions. A great differentiation in the parameters and structure of particular kinds of hair from the examined dogs was demonstrated. Thickness and length of hair of particular varieties showed statistically significant differentiation, as well as percentage contribution of particular hair fractions. As regard the content of chemical elements in particular hair fractions and dachshund variety, it appeared that the elements forming hair keratin were the most abundant. The findings presented in the paper may be a useful tool for the recognition of dachshund varieties based on their hair coat structure. They may be also a valuable contribution to the expanding breed standards, which lack histological hair description.
Treatment of venous leg ulcers with an ointment containing yarrow (Achillea millefolium) extract / Primena preparata sa ekstraktom hajdučke trave (Achillea millefolium) u lečenju venskih ulkusa potkolenice
Authors: Matić Milan , Đuran Verica , Jovanović Marina , Gajinov Zorica , Matić Aleksandra , Đuran Branislav , Pal Boža , Mimica-Dukić Neda 
Published: February 2013

Abstract
Traditional medicine credits yarrow (Achillea millefolium) with the ability to accelerate wound healing. The purpose of this research was to determine the effects of yarrow on the epithelization of the lower leg venous ulcers. The study included 39 patients with venous leg ulcers. They were divided into two groups: the first (experimental) group of patients were treated with an ointment containing 7.5% of yarrow extract. In the second (control) group, saline solution dressings were applied to ulcers, within the period of three weeks. In the experimental group, at the beginning of the therapy, the total surface of all the ulcers was 44736 mm2. After three weeks, the total surface of all the ulcers was 27000 mm2 (a decrease of 39.64%). In the control group, at the beginning of the therapy, the total surface of all the ulcers was 46116 mm2. At the end of the study (21 days) the total surface of all the ulcers was 39153 mm2 (a decrease of 15.1%). Herbal preparations are suitable for application in the therapy of venous ulcers, but their efficiency in wound healing is still to be investigated.
Risk of a second cutaneous primary melanoma and basal cell carcinoma in patients with a previous primary diagnosis of melanoma: true impact of dermoscopy follow-up in the identification of high-risk persons/ Značaj dermoskopskog praćenja u identifikaciji osoba s visokim rizikom od pojave drugog primarnog kutanog melanoma – prikaz slučaja
Authors: Drljević Irdina , Alendar Faruk 
Published: February 2013

Abstract
In clinical practice, positive personal history is considered to be as an indication of increased melanoma risk. The thickness of melanoma (Breslow Index) is the most important independent predicting factor of survival for stage I patients. We present a case of a second primary melanoma and basal cell carcinoma in a 48-year-old female patient with personal history of a superficial spreading melanoma located at the dorsal trunk with Breslow’s thickness of 0,5 mm and Clark’s II (stage IA) excised a year before, and a family background of melanoma. We would like to emphasize the benefits of digital dermatoscopy as well as teledermoscopy, and new teledermatology web services, in the follow-up of high-risk patients.
Peutz-Jegher’s Syndrome – A Case Report / Peutz-Jegher’s sindrom - prikaz slučaja
Authors: Sbutega Isidora , Popadić Svetlana , Ivanovski Petar , Arsov Biljana , Nikolić Miloš 
Published: February 2013

Abstract
Peutz-Jegher’s syndrome is a hereditary disorder characterized by melanocytic macules on the lips and buccal mucosa and multiple gastrointestinal hamartomatous polyps. It is caused by a mutation localized on chromosome 19p13.3. Skin and mucosal pigmentation may be present at birth and usually occur in early childhood, but occasionally may develop later. It is associated with an increased risk of malignancy for gastrointestinal carcinoma and also for breast, ovarian, testiscular, pancreatic and gallbladder cancer. We report a 12-year-old girl who presented with disseminated petty yellowish macules on the bridge of her nose, numerous brown to bluish black macules on her lips and buccal mucosa. Mucocutaneous pigmentation has been present from the age of five, with a negative family history. In our patient, esophageal endoscopy was normal, while the endoscopy of stomach and duodenum revealed multiple diminutive polyps. After clinical evaluation, there were no indications for therapy. Further follow up was suggested. Continuous surveillance is very important for patients with Peutz-Jegher’s syndrome in order to reduce risks of cancer and prevent other morbidity and mortality.