bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute GVHD in patients receiving IL-15/4-1BBL activated NK cells following T-cell–depleted stem cell transplantation
Authors: Shah Nirali N. , Baird Kristin , Delbrook Cynthia P. , Fleisher Thomas A. , Kohler Mark E. , Rampertaap Shakuntala , Lemberg Kimberly , Hurley Carolyn K. , Kleiner David E. , Merchant Melinda S. , Pittaluga Stefania , Sabatino Marianna , Stroncek David F. , Wayne Alan S. , Zhang Hua , Fry Terry J. , Mackall Crystal L. 
Published: December 2014

Key PointsAcute GVHD occurred in 5 of 9 patients after major histocompatibility–matched, T-cell–depleted peripheral blood stem cell transplantation plus IL-15/4-1BBL aNK-DLI. GVHD was more common in matched unrelated donor transplants and associated with higher CD3 chimerism, suggesting that aNK-DLI may augment T-cell alloreactivity.
Genetic inactivation of TRAF3 in canine and human B-cell lymphoma
Authors: Bushell Kevin R. , Kim Yukyoung , Chan Fong Chun , Ben-Neriah Susana , Jenks Andrew , Alcaide Miguel , Fornika Daniel , Grande Bruno M. , Arthur Sarah , Gascoyne Randy D. , Steidl Christian , Morin Ryan D. 
Published: December 2014

Key Points
TRAF3 is genetically inactivated in a substantial fraction of cBCLs. Focal genetic loss of TRAF3 is recurrent in human DLBCLs.
Strong convergence of iterative algorithms for the split equality problem
Authors: Shi Luo Yi , Chen Rudong , Wu Yujing 
Published: December 2014

Abstract
Let

H
1

,

H
2

,

H
3

be real Hilbert spaces,

C
⊆

H
1

,

Q
⊆

H
2

be two nonempty closed convex sets, and let

A
:

H
1

→

H
3

,

B
:

H
2

→

H
3

be two bounded linear operators. The split equality problem (SEP) is finding

x
∈
C

,

y
∈
Q

such that

A
x
=
B
y

. Recently, Moudafi has presented the ACQA algorithm and the RACQA algorithm to solve SEP. However, the two algorithms are weakly convergent. It is therefore the aim of this paper to construct new algorithms for SEP so that strong convergence is guaranteed. Firstly, we define the concept of the minimal norm solution of SEP. Using Tychonov regularization, we introduce two methods to get such a minimal norm solution. And then, we introduce two algorithms which are viewed as modifications of Moudafi’s ACQA, RACQA algorithms and KM-CQ algorithm, respectively, and converge strongly to a solution of SEP. More importantly, the modifications of Moudafi’s ACQA, RACQA algorithms converge strongly to the minimal norm solution of SEP. At last, we introduce some other algorithms which converge strongly to a solution of SEP.
Awareness and current implementation of drug dosage adjustment by pharmacists in patients with chronic kidney disease in Japan: a web-based survey
Authors: Kondo Yuki , Ishitsuka Yoichi , Shigemori Eri , Irikura Mitsuru , Kadowaki Daisuke , Hirata Sumio , Maemura Takeshi , Irie Tetsumi 
Published: December 2014

Abstract
Background
The aims of this study were to evaluate the current awareness of and implementation by pharmacists in Japan of adjustment of drug dosage according to renal function (ADDR) in patients with chronic kidney disease (CKD) and to clarify the factors influencing implementation of ADDR by community pharmacists.

Methods
We conducted a web-based questionnaire of Japanese community and hospital pharmacists. Responders were compared by characteristics, rate of implementation of ADDR, experience with adverse drug events, pharmacist awareness of implementation of ADDR, and obstacles to ADDR implementation experienced by pharmacists. Additionally, the factors influencing the implementation of ADDR by community pharmacists were investigated by logistic regression analysis.

Results
Fewer community pharmacists had implemented ADDR than hospital pharmacists. The community pharmacists had less experience with adverse drug events caused by an inappropriate dosage than the hospital pharmacists, while the hospital pharmacists had encountered more severe adverse drug events than the community pharmacists. The community pharmacists had less awareness of ADDR implementation, and believed that problems in implementing ADDR were caused by a lack of information on the renal function of patients. In the logistic regression analysis, the factors influencing implementation of ADDR were “Routinely receiving prescriptions from nephrologists”, “Experience with adverse drug events caused by inappropriate dosage for CKD patients”, and “Awareness of the need for pharmacists to check the dosage of renally excreted drugs”; they did not include “Lack of information on patient renal function”.

Conclusions
This study indicates that fewer Japanese community pharmacists than hospital pharmacists implement ADDR and that implementation of ADDR by community pharmacists is hindered by their limited awareness of the importance of patient renal function. We advocate that many countermeasures be introduced to prevent CKD patients from experiencing adverse drug events caused by inappropriate dosage. Such countermeasures would include a training program to educate pharmacists about the impact of impaired renal function on dosage of drugs that are excreted by the kidneys.
Genome-wide screening and functional analysis identify a large number of long noncoding RNAs involved in the sexual reproduction of rice
Authors: Zhang Yu-Chan , Liao Jian-You , Li Ze-Yuan , Yu Yang , Zhang Jin-Ping , Li Quan-Feng , Qu Liang-Hu , Shu Wen-Sheng , Chen Yue-Qin 
Published: December 2014

Abstract
Background
Long noncoding RNAs (lncRNAs) play important roles in a wide range of biological processes in mammals and plants. However, the systematic examination of lncRNAs in plants lags behind that in mammals. Recently, lncRNAs have been identified in Arabidopsis and wheat; however, no systematic screening of potential lncRNAs has been reported for the rice genome.

Results
In this study, we perform whole transcriptome strand-specific RNA sequencing (ssRNA-seq) of samples from rice anthers, pistils, and seeds 5 days after pollination and from shoots 14 days after germination. Using these data, together with 40 available rice RNA-seq datasets, we systematically analyze rice lncRNAs and definitively identify lncRNAs that are involved in the reproductive process. The results show that rice lncRNAs have some different characteristics compared to those of Arabidopsis and mammals and are expressed in a highly tissue-specific or stage-specific manner. We further verify the functions of a set of lncRNAs that are preferentially expressed in reproductive stages and identify several lncRNAs as competing endogenous RNAs (ceRNAs), which sequester miR160 or miR164 in a type of target mimicry. More importantly, one lncRNA, XLOC_057324, is demonstrated to play a role in panicle development and fertility. We also develop a source of rice lncRNA-associated insertional mutants.

Conclusions
Genome-wide screening and functional analysis enabled the identification of a set of lncRNAs that are involved in the sexual reproduction of rice. The results also provide a source of lncRNAs and associated insertional mutants in rice.
Integrated analyses of DNA methylation and hydroxymethylation reveal tumor suppressive roles of ECM1, ATF5, and EOMESin human hepatocellular carcinoma
Authors: Gao Fei , Xia Yudong , Wang Junwen , Lin Zhilong , Ou Ying , Liu Xing , Liu Weilong , Zhou Boping , Luo Huijuan , Zhou Baojin , Wen Bo , Zhang Xiuqing , Huang Jian 
Published: December 2014

Abstract
Background
Differences in 5-hydroxymethylcytosine, 5hmC, distributions may complicate previous observations of abnormal cytosine methylation statuses that are used for the identification of new tumor suppressor gene candidates that are relevant to human hepatocarcinogenesis. The simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine is likely to stimulate the discovery of aberrantly methylated genes with increased accuracy in human hepatocellular carcinoma.

Results
Here, we performed ultra-performance liquid chromatography/tandem mass spectrometry and single-base high-throughput sequencing, Hydroxymethylation and Methylation Sensitive Tag sequencing, HMST-seq, to synchronously measure these two modifications in human hepatocellular carcinoma samples. After identification of differentially methylated and hydroxymethylated genes in human hepatocellular carcinoma, we integrate DNA copy-number alterations, as determined using array-based comparative genomic hybridization data, with gene expression to identify genes that are potentially silenced by promoter hypermethylation.

Conclusions
We report a high enrichment of genes with epigenetic aberrations in cancer signaling pathways. Six genes were selected as tumor suppressor gene candidates, among which, ECM1, ATF5 and EOMES are confirmed via siRNA experiments to have potential anti-cancer functions.
Epigenetic modifications are associated with inter-species gene expression variation in primates
Authors: Zhou Xiang , Cain Carolyn E , Myrthil Marsha , Lewellen Noah , Michelini Katelyn , Davenport Emily R , Stephens Matthew , Pritchard Jonathan K , Gilad Yoav 
Published: December 2014

Abstract
Background
Changes in gene regulation have long been thought to play an important role in evolution and speciation, especially in primates. Over the past decade, comparative genomic studies have revealed extensive inter-species differences in gene expression levels, yet we know much less about the extent to which regulatory mechanisms differ between species.

Results
To begin addressing this gap, we perform a comparative epigenetic study in primate lymphoblastoid cell lines, to query the contribution of RNA polymerase II and four histone modifications, H3K4me1, H3K4me3, H3K27ac, and H3K27me3, to inter-species variation in gene expression levels. We find that inter-species differences in mark enrichment near transcription start sites are significantly more often associated with inter-species differences in the corresponding gene expression level than expected by chance alone. Interestingly, we also find that first-order interactions among the five marks, as well as chromatin states, do not markedly contribute to the degree of association between the marks and inter-species variation in gene expression levels, suggesting that the marginal effects of the five marks dominate this contribution.

Conclusions
Our observations suggest that epigenetic modifications are substantially associated with changes in gene expression levels among primates and may represent important molecular mechanisms in primate evolution.
Sentinel Node Biopsy After Neoadjuvant Chemotherapy in Biopsy-Proven Node-Positive Breast Cancer: The SN FNAC Study
Authors: Boileau Jean-Francois , Poirier Brigitte , Basik Mark , Holloway Claire M.B. , Gaboury Louis , Sideris Lucas , Meterissian Sarkis , Arnaout Angel , Brackstone Muriel , McCready David R. , Karp Stephen E. , Trop Isabelle , Lisbona Andre , Wright Frances C. , Younan Rami J. , Provencher Louise , Patocskai Erica , Omeroglu Atilla , Robidoux Andre 
Published: December 2014

Purpose An increasing proportion of patients (> 30%) with node-positive breast cancer will obtain an axillary pathologic complete response after neoadjuvant chemotherapy (NAC). If sentinel node (SN) biopsy (SNB) is accurate in this setting, completion node dissection (CND) morbidity could be avoided. Patients and Methods In the prospective multicentric SN FNAC study, patients with biopsy-proven node-positive breast cancer (T0-3, N1-2) underwent both SNB and CND. Immunohistochemistry (IHC) use was mandatory, and SN metastases of any size, including isolated tumor cells (ypN0[i+], ≤ 0.2 mm), were considered positive. The optimal SNB identification rate (IR) ≥ 90% and false-negative rate (FNR) ≤ 10% were predetermined. Results From March 2009 to December 2012, 153 patients were accrued to the study. The SNB IR was 87.6% (127 of 145; 95% CI, 82.2% to 93.0%), and the FNR was 8.4% (seven of 83; 95% CI, 2.4% to 14.4%). If SN ypN0(i+)s had been considered negative, the FNR would have increased to 13.3% (11 of 83; 95% CI, 6.0% to 20.6%). There was no correlation between size of SN metastases and rate of positive non-SNs. Using this method, 30.3% of patients could potentially avoid CND. Conclusion In biopsy-proven node-positive breast cancer after NAC, a low SNB FNR (8.4%) can be achieved with mandatory use of IHC. SN metastases of any size should be considered positive. The SNB IR was 87.6%, and in the presence of a technical failure, axillary node dissection should be performed. We recommend that SN evaluation with IHC be further evaluated before being included in future guidelines on the use of SNB after NAC in this setting.
Inherited Mutations in 17 Breast Cancer Susceptibility Genes Among a Large Triple-Negative Breast Cancer Cohort Unselected for Family History of Breast Cancer
Authors: Couch Fergus J. , Hart Steven N. , Sharma Priyanka , Toland Amanda Ewart , Wang Xianshu , Miron Penelope , Olson Janet E. , Godwin Andrew K. , Pankratz V. Shane , Olswold Curtis , Slettedahl Seth , Hallberg Emily , Guidugli Lucia , Davila Jaime I. , Beckmann Matthias W. , Janni Wolfgang , Rack Brigitte , Ekici Arif B. , Slamon Dennis J. , Konstantopoulou Irene , Fostira Florentia , Vratimos Athanassios , Fountzilas George , Pelttari Liisa M. , Tapper William J. , Durcan Lorraine , Cross Simon S. , Pilarski Robert , Shapiro Charles L. , Klemp Jennifer , Yao Song , Garber Judy , Cox Angela , Brauch Hiltrud , Ambrosone Christine , Nevanlinna Heli , Yannoukakos Drakoulis , Slager Susan L. , Vachon Celine M. , Eccles Diana M. , Fasching Peter A. 
Published: December 2014

Purpose Recent advances in DNA sequencing have led to the development of breast cancer susceptibility gene panels for germline genetic testing of patients. We assessed the frequency of mutations in 17 predisposition genes, including BRCA1 and BRCA2, in a large cohort of patients with triple-negative breast cancer (TNBC) unselected for family history of breast or ovarian cancer to determine the utility of germline genetic testing for those with TNBC. Patients and Methods Patients with TNBC (N = 1,824) unselected for family history of breast or ovarian cancer were recruited through 12 studies, and germline DNA was sequenced to identify mutations. Results Deleterious mutations were identified in 14.6% of all patients. Of these, 11.2% had mutations in the BRCA1 (8.5%) and BRCA2 (2.7%) genes. Deleterious mutations in 15 other predisposition genes were detected in 3.7% of patients, with the majority observed in genes involved in homologous recombination, including PALB2 (1.2%) and BARD1, RAD51D, RAD51C, and BRIP1 (0.3% to 0.5%). Patients with TNBC with mutations were diagnosed at an earlier age (P < .001) and had higher-grade tumors (P = .01) than those without mutations. Conclusion Deleterious mutations in predisposition genes are present at high frequency in patients with TNBC unselected for family history of cancer. Mutation prevalence estimates suggest that patients with TNBC, regardless of age at diagnosis or family history of cancer, should be considered for germline genetic testing of BRCA1 and BRCA2. Although mutations in other predisposition genes are observed among patients with TNBC, better cancer risk estimates are needed before these mutations are used for clinical risk assessment in relatives.
Nivolumab for Metastatic Renal Cell Carcinoma: Results of a Randomized Phase II Trial
Authors: Motzer Robert J. , Rini Brian I. , McDermott David F. , Redman Bruce G. , Kuzel Timothy M. , Harrison Michael R. , Vaishampayan Ulka N. , Drabkin Harry A. , George Saby , Logan Theodore F. , Margolin Kim A. , Plimack Elizabeth R. , Lambert Alexandre M. , Waxman Ian M. , Hammers Hans J. 
Published: December 2014

PurposeNivolumab is a fully human immunoglobulin G4 programmed death–1 immune checkpoint inhibitor antibody that restores T-cell immune activity. This phase II trial assessed the antitumor activity, dose-response relationship, and safety of nivolumab in patients with metastatic renal cell carcinoma (mRCC).Patients and MethodsPatients with clear-cell mRCC previously treated with agents targeting the vascular endothelial growth factor pathway were randomly assigned (blinded ratio of 1:1:1) to nivolumab 0.3, 2, or 10 mg/kg intravenously once every 3 weeks. The primary objective was to evaluate the dose-response relationship as measured by progression-free survival (PFS); secondary end points included objective response rate (ORR), overall survival (OS), and safety.ResultsA total of 168 patients were randomly assigned to the nivolumab 0.3- (n = 60), 2- (n = 54), and 10-mg/kg (n = 54) cohorts. One hundred eighteen patients (70%) had received more than one prior systemic regimen. Median PFS was 2.7, 4.0, and 4.2 months, respectively (P = .9). Respective ORRs were 20%, 22%, and 20%. Median OS was 18.2 months (80% CI, 16.2 to 24.0 months), 25.5 months (80% CI, 19.8 to 28.8 months), and 24.7 months (80% CI, 15.3 to 26.0 months), respectively. The most common treatment-related adverse event (AE) was fatigue (24%, 22%, and 35%, respectively). Nineteen patients (11%) experienced grade 3 to 4 treatment-related AEs.ConclusionNivolumab demonstrated antitumor activity with a manageable safety profile across the three doses studied in mRCC. No dose-response relationship was detected as measured by PFS. These efficacy and safety results in mRCC support study in the phase III setting.
Slow progression of exudative age related macular degeneration associated with hypertrophy of the retinal pigment epithelium
Authors: Stern Jeffrey , Eveleth David , Masula Jennifer , Temple Sally 
Published: December 2014

Rationale: Choroidal neovascular (CNV) lesions in younger patients are often accompanied by the appearance of a surrounding ring of pigment that is associated with disease regression or slowed disease progression. In older patients with age-related macular degeneration (AMD), however, hypertrophy of the retinal pigment epithelium (RPE) is known to occur but has not previously been reported to be associated with CNV regression. This report describes the clinical course of a case series of AMD patients with pigment hypertrophy adjacent to CNV associated with stabilization of the CNV lesion.Methods: A retrospective analysis of exudative AMD patients seen by a single retina specialist over a 7-year period.Results: Retrospective analysis of 955 exudative AMD patients revealed pigment hypertrophy associated with CNV in 33 patients. A ring of pigment surrounded CNV in 6 of these. Three representative patients are presented to illustrate the decrease in macular edema, reduced fluorescein leakage and slowed CNV progression that was associated with a pigment ring around CNV in AMD. Pigment hypertrophy was associated with blocked fluorescein leakage and exudative AMD patients with a complete pigment ring maintained stable visual acuity, macular edema, fluorescein leakage and CNV lesion size without treatment for intervals of up to 21 months. Conclusion: We report slowed disease progression in AMD patients who develop pigment around CNV. The slow rate of disease progression in the AMD patient subgroup having a pigment ring is a factor to consider in determining the treatment interval for exudative AMD patients.
Prospects of Polytechnic Principle of Learning in Technological Training of Pupils of 5-7 Grades
Authors: Терентьев  , Terentyev Sergey 
Published: December 2014

This article discusses the problem of training future
personnel forScience and Engineering. The solution of this problem
is important for modern education. Answers to the questions
posed by the problem can be found in the polytechnic education.
Identification of aims of polytechnic education and learning is
one of the goals of this work.
Currently there is no unified acknowledged concept of polytech -
nic education. The experimental part of this work demonstrates
the application of the principle of polytechnic education at technology
lessons for grades 5-7. The outcome of the experiment was
the data of interview and questionnaire. From this data, we can
speak about the effectiveness of the principle of polytechnic education
in technological training of students. Most effectively
polytechnic principle of learning has proved itself duringapplication
of problem and project methods. Thus, we can speak about
the relevance of its use in the construction of a modern lesson
content.
Skills Development and Retraining by Means of Distance Learning Technologies
Authors: Лабутин  , Labutin V. 
Published: December 2014

This article discusses some of the key aspects of the organization
of additional education with the use of distance learning
technologies of workers of pedagogical field.
This article is based on the experience of organization of skills
development and retraining of teachers and administrators of schools
of the Moscow region.
The paper outlines the key aims and objectives of the application
of distance learning technology in the organization of advanced
education. The author notes the need to develop a special educational
environment and discloses conditions of the organization of
work of students, teachers and curriculum developers. Functional
units of the educational institutions of additional professional
education and its regional offices are reviewed.
The most effective forms of training activities in terms of information-
educational environment are outlined.
Modern Educational Pedagogical Technologies as a Factor of Improving Quality of Education
Authors: Горских  , Gorskikh E. 
Published: December 2014

The learning process is a process of management, i.e.
impact on educational system, organization of knowledge. For its
successful implementation teaching science develops models to
promote good governance in educational systems. These include
special methods (techniques) and training technologies. The paper
presents educational technologies as a factor of improving quality
of education in general education and particularly in primary school.
Yaroslavl area regional tourist product: media information and consumer opinion
Authors: Кривошеева Татьяна , Krivosheeva Tatiana 
Published: December 2014

The article contents the brief results of an Yaroslavl area regional tourist product study at the media and similar sources informing position, it’s perception of potential and real consumers. The research was carried out in two directions: a publications content analysis and Internet users pool.
According to the tourist service consumers, the purposes of content analysis was to create the Yaroslavl region sites and collective accommodation facilities rating, as well as to determinate the main factors of tourism development restraining and the favorable perception of the Yaroslavl region as a tourist area reducing
Web-pool was aimed at determining the tourist trips frequency, including the Russian Federation territory; identifying consumer preferences by type of recreation; identify the most popular tourist interest areas for different age groups; identification of the most popular information on tour objects sources; determination the reasons of consumers choice of one or another travel company; consumer requirements for infrastructural components of tourism products definition. The special attention was paid to the issues: what is laid in the basis of various age categories tourists experience formation.
The article has the Yaroslavl region tourism development strategy prospects, in accordance with the social-economic development strategy/ More it contents the proposals on the regional tourism service system sphere formation.