bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased serum levels of interleukin-8 in patients with tension-type headache
Authors: Domingues Renan B , Duarte Halina , Rocha Natália P , Teixeira Antonio L 
Published: November 2014

Background and objectives The pathophysiology of tension-type headache is not well understood. Increased peripheral levels of pro-inflammatory cytokines may act as mediators of several chronic pain disorders. The aim of the present study was to investigate the peripheral levels of chemokines in patients with tension-type headache. Methods This was a cross sectional study evaluating serum levels of chemokines in age and sex-matched tension-type headache patients, ictally and interictally, and control participants. Beck Depression and Anxiety Inventories were recorded. Serum levels of monocyte chemoattractant protein-1, macrophage inflammatory protein 1α, regulated on activation, normal T cell expressed and secreted, eotaxin, eotaxin-2, interleukin-8, interferon gamma induced protein-10 were measured by enzyme-linked immunosorbent assay. Results A total of 96 participants (48 tension-type headache, 48 controls) were included. Interleukin-8 levels were significantly increased in patients with tension-type headache when compared to controls (413.8 (123.4–1756.3) and 329 (107.8–955.6), respectively, P = 0.025). Anxiety and depression scores were higher in patients with tension-type headache but interleukin-8 increase in tension-type headache patients persisted after controlling for anxiety and depression symptoms. Patients with headache at the time of assessment had increased monocyte chemoattractant protein-1 levels when compared with patients without headache (2809.3 (1101–6122.2) and 1630.2 (669.3–31056.8), respectively P = 0.026). Patients with episodic and chronic tension-type headache had no significant differences in serum chemokines levels. Conclusion Interleukin-8 was increased in tension-type headache and monocyte chemoattractant protein-1 was higher in tension-type headache patients with headache, suggesting that pro-inflammatory mechanisms may participate in tension-type headache pathophysiology.
The association of serum anti-ribosomal P antibody with clinical and serological disorders in systemic lupus erythematosus: a systematic review and meta-analysis
Authors: Shi Z-R , Cao C-X , Tan G-Z , Wang L 
Published: November 2014

Objective Anti-ribosomal P (anti-P) antibody is a serological specific marker of systemic lupus erythematosus (SLE). The aim of this study is to investigate the association of this antibody with clinical and serological disorders in SLE. Methods All relevant literature was retrieved from PubMed, EMBASE, Web of Science and CNKI databases. The qualities of these studies were evaluated using a modified version of the Newcastle–Ottawa scale. The associations of anti-P antibody with clinical and serological disorders were determined by the pooled odds ratio (OR) and the confidence interval (CI) calculated using meta-analysis with the Mantel–Haenszel method. Results Sixteen cohort studies with 2355 patients were included in this study. Malar rash, oral ulcer and photosensitivity were strongly associated with serum anti-P antibody, with OR (95% CI) values of 2.05 (1.42–2.92), 1.49 (1.05–2.13) and 1.44 (1.08–1.91), respectively. Arthritis and renal involvement were not associated with anti-P antibody, whereas a high heterogeneity was observed due to ethnicity and publication bias, respectively. Neuropsychiatric SLE (NPSLE), hepatic involvement, anti-dsDNA, anti-Sm and anti-cardiolipin antibodies (aCL) were observed more frequently in anti-P positive patients than in negative patients. Studies on hepatic involvement showed a low precision with substantially broad CI (2.56–11.2). A high heterogeneity presented among studies on NPSLE, anti-Sm and aCL. Conclusions Anti-P antibody is significantly associated with malar rash, oral ulcer, photosensitivity and serum anti-dsDNA antibody, and potentially associated with NPSLE, hepatic damage, serum anti-Sm and aCL.
Predicting the stage shift as a result of breast cancer screening in low- and middle-income countries: a proof of concept
Authors: Zelle Sten G. , Baltussen Rob , Otten Johannes D.M. , Heijnsdijk Eveline A.M. , van Schoor Guido , Broeders Mireille J.M. 
Published: November 2014

Objective To provide proof of concept for a simple model to estimate the stage shift as a result of breast cancer screening in low- and middle-income countries (LMICs). Stage shift is an essential early detection indicator and an important proxy for the performance and possible further impact of screening programmes. Our model could help LIMCs to choose appropriate control strategies. Methods We assessed our model concept in three steps. First, we calculated the proportional performance rates ( i.e. index number Z) based on 16 screening rounds of the Nijmegen Screening Program (384,884 screened women). Second, we used linear regression to assess the association between Z and the amount of stage shift observed in the programme. Third, we hypothesized how Z could be used to estimate the stage shift as a result of breast cancer screening in LMICs. Results Stage shifts can be estimated by the proportional performance rates (Zs) using linear regression. Zs calculated for each screening round are highly associated with the observed stage shifts in the Nijmegen Screening Program (Pearson’s R: 0.798, R square: 0.637). Conclusions Our model can predict the stage shifts in the Nijmegen Screening Program, and could be applied to settings with different characteristics, although it should not be straightforwardly used to estimate the impact on mortality. Further research should investigate the extrapolation of our model to other settings. As stage shift is an essential screening performance indicator, our model could provide important information on the performance of breast cancer screening programmes that LMICs consider implementing.
Engagement Through Praxis in Educational Game Design
Authors: Ruggiero Dana , Watson William R. 
Published: November 2014

Background. Engagement in praxis, the process of acting and reflecting in a cyclical fashion, fosters the development of critical thinking and problem-solving skills among educational game designers. Resulting from this process, designers can apply their learned skills to create highly engaging educational games. Aim. This study examines the game development process of 22 international game designers from business, government, and academia as they describe their engagement in praxis when designing educational games. Method. Building upon research on reflective practice, this article provides insights on how engagement in praxis can be framed as a tool for educational game design that naturally promotes learning and motivation during gameplay. The researchers examine common threads that illustrate the process that educational video game designers use to create games. Results. Four common threads or themes were pulled from in-depth interviews with each designer. In this article, we focus on engagement in designing and applying game mechanics, engagement in content and context in educational games, feedback as an indicator of motivation and engagement, and working within project constraints to create engaging experiences.
Spontaneous gastrointestinal perforation in cats: a retrospective study of 13 cases
Authors: Bernardin Fanny , Martinez Rivera Laura , Ragetly Guillaume , Gomes Eymeric , Hernandez Juan 
Published: November 2014

Objectives The aim of this study was to describe the clinical characteristics and the frequency of malignant vs non-malignant causes for spontaneous gastrointestinal perforation in cats. Methods The medical records of cats diagnosed as having gastrointestinal perforation between August 2010 and July 2013 were reviewed. Diagnosis was confirmed by exploratory surgery. Patients with incomplete records, perforation due to external trauma, leakage at previous enterotomy or anastomotic sites, or foreign bodies were excluded. Each record was examined for different information pertaining to signalment, medical history, clinical and clinicopathological data, imaging findings, abdominal fluid examination, surgical findings, histopathological examination, treatment received after surgery and outcome. Results Thirteen cats were included. Five of these cats had concurrent illnesses, including viral upper respiratory tract disease, pancreatitis and chronic kidney disease. Two cats had previously received non-steroidal anti-inflammatory drugs and four had received corticosteroids. Clinical signs and clinicopathological abnormalities were not specific. Six of 13 patients were diagnosed during surgery with gastric perforations, four patients with duodenal perforations and three patients with jejunal perforations. Histopathological examination of the ulcerated wall was performed in 11/13 cats. Alimentary lymphoma was diagnosed in six cats. Non-neoplastic lesions (lymphocytic– plasmacytic inflammatory bowel disease, necrotic suppurative enteritis) were observed in the other five cats. The major limitation of the study was the small sample size. Conclusions and relevance Lymphoma may be a frequent cause of spontaneous perforation in cats. Therefore, histological examination of ulceration is essential in all cases. The direct and sole implication of anti-inflammatory administration in a gastrointestinal perforation is not clearly established in this study.
The intron-22–inverted F8 locus permits factor VIII synthesis: explanation for low inhibitor risk and a role for pharmacogenomics
Authors: Sauna Zuben E. , Lozier Jay N. , Kasper Carol K. , Yanover Chen , Nichols Timothy , Howard Tom E. 
Published: November 2014

Abstract
Intron-22-inversion patients express the entire Factor VIII (FVIII)-amino-acid sequence intracellularly as 2 non-secreted polypeptides and have a positive “intracellular (I)-FVIII-CRM” status. Mutations conferring a positive I-FVIII-CRM status are associated with low inhibitor risk and are pharmacogenetically relevant because inhibitor risk may be affected by the nature of the therapeutic FVIII-protein (tFVIII), the affinity of any tFVIII-derived foreign peptide (tFVIII-fp) for any HLA class-II isomer (HLA-II) comprising individual major histocompatibility complex (MHC) repertoires, and the stability of any tFVIII-fp/HLA-II complex. We hypothesize that mutations conferring a completely or substantially negative I-FVIII-CRM status are pharmacogenetically irrelevant because inhibitor risk is high with any tFVIII and individual MHC repertoire.
Epigenetic and in vivo comparison of diverse MSC sources reveals an endochondral signature for human hematopoietic niche formation
Authors: Reinisch Andreas , Etchart Nathalie , Thomas Daniel , Hofmann Nicole A. , Fruehwirth Margareta , Sinha Subarna , Chan Charles K. , Senarath-Yapa Kshemendra , Seo Eun-Young , Wearda Taylor , Hartwig Udo F. , Beham-Schmid Christine , Trajanoski Slave , Lin Qiong , Wagner Wolfgang , Dullin Christian , Alves Frauke , Andreeff Michael , Weissman Irving L. , Longaker Michael T. , Schallmoser Katharina , Majeti Ravindra , Strunk Dirk 
Published: November 2014

Key Points
Epigenetics and in vivo behavior can distinguish MSCs from different sources. BM-derived MSCs form a hematopoietic niche via a vascularized cartilage intermediate.
Observation and tunability of room temperature photoluminescence of GaAs/GaInAs core-multiple-quantum-well shell nanowire structure grown on Si (100) by molecular beam epitaxy
Authors: Park Kwang Wook , Park Chang Young , Ravindran Sooraj , Jang Ja-Soon , Jo Yong-Ryun , Kim Bong-Joong , Lee Yong Tak 
Published: November 2014

Abstract
We report the observation of room temperature photoluminescence (PL) emission from GaAs/GaInAs core-multiple-quantum-well (MQW) shell nanowires (NWs) surrounded by AlGaAs grown by molecular beam epitaxy (MBE) using a self-catalyzed technique. PL spectra of the sample show two PL peaks, originating from the GaAs core NWs and the GaInAs MQW shells. The PL peak from the shell structure red-shifts with increasing well width, and the peak position can be tuned by adjusting the width of the MQW shell. The GaAs/GaInAs core-MQW shell NW surrounded by AlGaAs also shows an enhanced PL intensity due to the improved carrier confinement owing to the presence of an AlGaAs clad layer. The inclined growth of the GaAs NWs produces a core-MQW shell structure having a different PL peak position than that of planar QWs. The PL emission by MQW shell and the ability to tune the PL peak position by varying the shell width make such core-shell NWs highly attractive for realizing next generation ultrasmall light sources and other optoelectronics devices.

PACS

81.07.Gf; 81.15.Hi; 78.55.Cr
Optogenetic Activation of Brainstem Serotonergic Neurons Induces Persistent Pain Sensitization
Authors: Cai You-Qing , Wang Wei , Hou Yuan-Yuan , Pan Zhizhong Z 
Published: November 2014

Background: The rostral ventromedial medulla (RVM) is a key brainstem structure that conveys powerful descending influence of the central pain-modulating system on spinal pain transmission and processing. Serotonergic (5-HT) neurons are a major component in the heterogeneous populations of RVM neurons and in the descending pathways from RVM. However, the descending influence of RVM 5-HT neurons on pain behaviors remains unclear. Results: In this study using optogenetic stimulation in tryptophan hydroxylase 2 (TPH2)-Channelrhodopsin 2 (ChR2) transgenic mice, we determined the behavioral effects of selective activation of RVM 5-HT neurons on mechanical and thermal pain behaviors in vivo. We found that ChR2-EYFP-positive neurons strongly co-localized with TPH2-positive (5-HT) neurons in RVM. Optogenetic stimulation significantly increased c-fos expression in 5-HT cells in the RVM of TPH2-ChR2 mice, but not in wild type mice. Behaviorally, the optogenetic stimulation decreased both mechanical and thermal pain threshold in an intensity-dependent manner, with repeated stimulation producing sensitized pain behavior for up to two weeks. Conclusions: These results suggest that selective activation of RVM 5-HT neurons exerts a predominant effect of pain facilitation under control conditions.
Diagnostic and prognostic value of neutrophil gelatinase-associated lipocalin, matrix metalloproteinase-9, and tissue inhibitor of matrix metalloproteinases-1 for sepsis in the Emergency Department: an observational study
Authors: Wang Miaomiao , Zhang Qian , Zhao Xin , Dong Guijuan , Li Chunsheng 
Published: November 2014

Abstract
Introduction
The aim of this study was to evaluate the early diagnostic, risk stratification and prognostic value of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of matrix metalloproteinases-1 (TIMP-1), compared with procalcitonin (PCT) and the Mortality in Emergency Department Sepsis (MEDS) score in septic patients in the emergency department (ED).

Methods
In total, 480 consecutive adult patients were enrolled in this study. They fulfilled the systemic inflammatory response syndrome (SIRS) criteria and were admitted to the ED of Beijing Chaoyang Hospital from February 2013 to August 2013. A total of 40 healthy controls comprised the control group. The patients were classified into four groups: SIRS, sepsis, severe sepsis, and septic shock. Serum NGAL, MMP-9, TIMP-1 and PCT were measured, and MEDS score was calculated at enrollment. The prognostic values of NGAL, MMP-9 and TIMP-1 were compared with PCT and MEDS score. A 28-day follow-up was performed for all patients.

Results
The median levels of serum NGAL and TIMP-1 increased with sepsis severity. The areas under the receiver operating characteristic (AUC) curves of NGAL or TIMP-1 were greater than those of PCT and MEDS score in diagnosing and predicting 28-day mortality, and the AUC of a combination of NGAL and MEDS score or TIMP-1 and MEDS score was more significant. Serum NGAL, MMP-9 and TIMP-1 levels were significantly higher in non-survivors than survivors at 28 days’ follow-up. In addition, the level of NGAL was much higher in septic patients with acute kidney injury (AKI) than those without AKI. NGAL, TIMP-1, MMP-9 and MEDS score were found to be independent predictors of 28-day mortality in septic patients. The levels of serum NGAL and TIMP-1 were positively correlated with PCT and MEDS score in every septic group.

Conclusions
NGAL and TIMP-1 are valuable for the risk stratification, early diagnosis and prognostication of sepsis in the ED. NGAL is also a valuable biomarker for prognosis of septic patients with AKI in the ED.
Comparison of binder compositions in Pompeian wall painting styles from Insula Occidentalis
Authors: Gelzo Monica , Grimaldi Mario , Vergara Alessandro , Severino Valeria , Chambery Angela , Dello Russo Antonio , Piccioli Ciro , Corso Gaetano , Arcari Paolo 
Published: November 2014

Abstract

Background
Although the pigment composition of Pompeian wall paintings has been the object of several studies, a comprehensive characterization of paint binder components is still lacking. This work aimed investigated at a molecular level the binder composition differences among wall paintings belonging to different periods of Pompeii's history. Analytical investigations were performed on representative samples of the first, second, third, and fourth painting styles excavated from the house of Marcus Fabius Rufus (Insula Occidentalis). The application of sensitive experimental methodologies was complemented by historical knowledge to gain insight in painting techniques and materials used by Pompeian artists.

Results
Fourier transform infrared spectroscopy and Raman spectroscopy were used to investigate the organic components and pigments present in powders obtained from samples of the four painting styles. No proteinaceous components were detected in the samples with liquid chromatography-electrospray ionization-hybrid quadrupole/time-of-flight mass spectrometry. Liquid chromatography, gas chromatography with flame-ionization detection, and gas chromatography–mass spectrometry of polar and non-polar components extracted from powders were used to evaluate and compare the free amino acids, sugars, and fatty acids profiles.

Conclusions
Pigments and natural products (lipids, gums and wheat flours) were the main components of all samples. This supports the hypothesis that artists likely used water tempera for Pompeian wall paintings.
Forecasting product returns for remanufacturing systems
Authors: Liang Xavier , Jin Xiaoning , Ni Jun 
Published: November 2014

Abstract
One of the major challenges that a remanufacturer faces at strategic planning level today is to match its supply (returned items) with demand due to the inherited uncertainties and variations on both sides. Forecasting product returns is one of the most important tasks of this matching process. Unlike forecasting for traditional manufacturing systems, both quantity and quality forecasts are critical since return timing, quantity, and the quality of returned products can all vary dramatically. This research develops a forecasting method which incorporates knowledge from related sales, product usage, customer return behavior, and product life expectancy information to provide a more accurate prediction of product returns. The models are validated using Monte Carlo simulations. Numerical cases are also presented to illustrate its usage and some important insights.
Investment casting of nozzle guide vanes from nickel-based superalloys: part I – thermal calibration and porosity prediction
Authors: Torroba Agustín Jose , Koeser Ole , Calba Loic , Maestro Laura , Carreño-Morelli Efrain , Rahimian Mehdi , Milenkovic Srdjan , Sabirov Ilchat , LLorca Javier 
Published: November 2014

AbstractInvestment casting is the only commercially used technique for fabrication of nozzle guide vanes (NGVs), which are one of the most important structural parts of gas turbines. Manufacturing of NGVs has always been a challenging task due to their complex shape. This work focuses on development of a simulation tool for investment casting of a new generation NGV from MAR-M247 Ni-based superalloy. A thermal model is developed to predict thermal history during investment casting. Experimental casting trials of the NGV are carried out and the thermal history of metal, mold, and insulation wrap is recorded. Inverse modeling of the casting trials is used to define accurately some thermophysical parameters and boundary conditions of the thermal model. Based on the validated thermal model, another model is developed to predict porosity in the as-cast NGVs. The porosity predictions are in good agreement with the experimental results in the as-cast NGVs. The advantages and shortcomings of the developed modeling tool are discussed.
Length-dependent anisotropic scaling of spindle shape
Authors: Young Sarah , Besson Sébastien , Welburn Julie P. I. 
Published: November 2014

ABSTRACT
Spindle length varies dramatically across species and during early development to segregate chromosomes optimally. Both intrinsic factors, such as regulatory molecules, and extrinsic factors, such as cytoplasmic volume, determine spindle length scaling. However, the properties that govern spindle shape and whether these features can be modulated remain unknown. Here, we analyzed quantitatively how the molecular players which regulate microtubule dynamics control the kinetics of spindle formation and shape. We find that, in absence of Clasp1 and Clasp2, spindle assembly is biphasic due to unopposed inward pulling forces from the kinetochore-fibers and that kinetochore-fibers also alter spindle geometry. We demonstrate that spindle shape scaling is independent of the nature of the molecules that regulate dynamic microtubule properties, but is dependent on the steady-state metaphase spindle length. The shape of the spindle scales anisotropically with increasing length. Our results suggest that intrinsic mechanisms control the shape of the spindle to ensure the efficient capture and alignment of chromosomes independently of spindle length.
Recruitment Into a Cessation Trial Via the New Zealand Quitline: Many Benefits, Few Limitations
Authors: Bullen Chris , Howe Colin , Grigg Michele , Phillips Frances , Silcock Rose , Glover Marewa , McRobbie Hayden , Whittaker Robyn 
Published: November 2014

AbstractObjective: To report on the use of the New Zealand Quitline for recruiting participants to a smoking cessation trial. Methods: Analysis of data on trial recruitment and randomisation. Results: 68% of 26,369 callers to the New Zealand Quitline over 12 months indicated an interest in taking part in research, 28% of whom met eligibility criteria for a cessation intervention trial, assessed on the data routinely collected at Quitline registration. Of these, 1317 (26%) were contacted by call back with 1027 (78%) agreeing to take part in the trial. After further eligibility checking 851 people were randomised. Weighting of calls ensured that 25% of participants were Maori. Conclusions: Quitlines have good potential to be an effective means of randomising participants into cessation trials and ensuring adequate representation of underrepresented population groups.