bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel circular RNA hsa_circ_0036683 suppresses proliferation and migration by mediating the miR‐4664‐3p/CDK2AP2 axis in non‐small cell lung cancer
Authors: Liu Rui , Zhang Han , Xin Jiaxuan , Xie Shu‐yang , Jiao Fei , Li You‐Jie , Chu Meng‐yuan , Qiu Junming , Yan Yun‐fei 
Published: August 2024

AbstractBackgroundThe aim of the present study was to investigate the function of novel circular RNA hsa_circ_0036683 (circ‐36683) in non‐small cell lung cancer (NSCLC).MethodsRNA sequencing was used to screen out differentially expressed miRNAs. Expression levels of miR‐4664‐3p and circ‐36683 were evaluated in lung carcinoma cells and tissues by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR). The effects of miR‐4664‐3p and circ‐36683 on proliferation and migration were assessed using cell counting kit‐8 (CCK‐8), wound healing and transwell migration assays and xenograft experiments. The targeting relationship of circ‐36683/miR‐4664‐3p/CDK2AP2 was assessed by luciferase reporter assays, western blot, qRT‐PCR and argonaute2‐RNA immunoprecipitation (AGO2 RIP). Co‐immunoprecipitation (Co‐IP), 5‐ethynyl‐2′‐deoxyuridine (EdU) staining and CCK‐8 were used to validate the indispensable role of CDK2AP2 in suppressing cell proliferation as a result of CDK2AP1 overexpression.ResultsBy RNA sequencing, miR‐4664‐3p was screened out as an abnormally elevated miRNA in NSCLC tissues. Transfection of miR‐4664‐3p could promote cell proliferation, migration and xenograft tumor growth. As a target of miR‐4664‐3p, CDK2AP2 expression was downregulated by miR‐4664‐3p transfection and CDK2AP2 overexpression could abolish the proliferation promotion resulting from miR‐4664‐3p elevation. Circ‐36683, derived from back splicing of ABHD2 pre‐mRNA, was attenuated in NSCLC tissue and identified as a sponge of miR‐4664‐3p. The functional study revealed that circ‐36683 overexpression suppressed cell proliferation, migration and resulted in G0/G1 phase arrest. More importantly, the antioncogenic function of circ‐36683 was largely dependent on the miR‐4664‐3p/CDK2AP2 axis, through which circ‐36683 could upregulate the expression of p53/p21/p27 and downregulate the expression of CDK2/cyclin E1.ConclusionThe present study revealed the antioncogenic role of circ‐36683 in suppressing cell proliferation and migration and highlighted that targeting the circ‐36683/miR‐4664‐3p/CDK2AP2 axis is a promising strategy for the intervention of NSCLC.
Helicopter emergency medical services in Iceland between 2018 and 2022–A retrospective study
Authors: Stefansson Sigurjon Orn , Magnusson Vidar , Sigurdsson Martin I. 
Published: August 2024

AbstractBackgroundHelicopter emergency services (HEMS) are widely used to bring medical assistance to individuals that cannot be reached by other means or individuals that have time‐critical medical conditions, such as chest pain, stroke or severe trauma. It is a very expensive resource whose use and importance depends on local conditions. The aim of this study was to describe flight and patient characteristics in all HEMS flights done in Iceland, a geographically isolated, mountainous and sparsely populated country, over a 5‐year course.MethodsThis retrospective study included all individuals requiring HEMS transportation in Iceland during 2018–2022. The electronic database of the Icelandic Coast Guard was used to identify the individuals and register flight data. Electronic databases from Landspitali and Akureyri hospitals were used to collect clinical variables. Descriptive statistics was applied.ResultsThe average number of HEMS transports was 3.5/10,000 inhabitants and the median [IQR] activation time and flight times were 30 min [20–42] and 40 min [26–62] respectively. The vast majority of patients were transported to Landspitali Hospital in Reykjavik. More than half of the transports were due to trauma, the most common medical transports were due to chest pain or cardiac arrests. Advanced medical therapy was provided for 66 (10%) of individuals during primary transports, 157 (24%) of individuals were admitted to intensive care, 188 (28%) needed surgery and 53 (7.9%) needed a coronary angiography.ConclusionIn Iceland, the number of transports is lower but activation and flight times for HEMS flights are considerably longer than in other Nordic countries, likely due to geographical features and the structure of the service including utilizing helicopters both for HEMS and search and rescue operations. The transport times for some time‐sensitive conditions are not within standards set by international studies and guidelines.
The effect of government‐guided funds on target industries in development zones – Evidence from China
Authors: Xiao Fulong , Liang Zini , Lv Yongbin , Wang Wei 
Published: August 2024

AbstractThe interplay between state‐led interventions and market‐driven forces has gained unparalleled prominence. This paper examines China's strategic use of government‐guided funds (GGFs) and their consequential impact on its national high‐tech zones (NHZs). Through an analysis of 1599 annual observations from 123 NHZs spanning 2007–2019, we reveal a compelling correlation between GGF investments and augmented industrial activity. Our findings indicate that NHZs benefitting from GGF allocations evidenced a significant 5.5% surge in industrial activity. Two underlying mechanisms drive this relationship. The leverage mechanism portrays GGFs as attractors of private social capital to tech‐centric ventures within NHZs. Meanwhile, the guidance mechanism underscores NHZs' strategic clustering of high‐tech sectors, amplifying benefits from shared resources and knowledge. Empirical analysis affirms both mechanisms, showing NHZs with robust social capital magnetism and tech clustering derive pronounced gains from GGF interventions. Our results carry practical implications for both policymakers and academicians, offering a nuanced understanding of the symbiotic relationship between fiscal strategies and industrial growth.
Pregnancy and stomas: a 9‐year retrospective series at a major metropolitan hospital in Brisbane Queensland
Authors: Kelly Madeleine Louise , Fullerton Alexandra , Cao Amy Millicent Yesheng , Colbran Rachel , Kimble Rebecca , Clark David A. 
Published: August 2024

AbstractBackgroundOver 42 000 Australians live with a stoma, and this number increases annually. Pregnancy in stoma patients is a rare but complex condition and there is limited published literature regarding surgical and obstetric complications in pregnant stoma patients. The aim of this paper was to review stoma outcomes, perinatal morbidity and mortality, and early postpartum period in pregnant stoma patients.MethodsData was retrospectively obtained on women of childbearing age, with a stoma, who had been pregnant and birthed in the last nine years at the Royal Brisbane and Women's Hospital between January 2014 to December 2022. Data recorded included patient demographics, type of stoma, indication for stoma, need for additional abdominal surgeries, method of conception, pregnancy complications, length of stay, neonatal outcomes and post pregnancy stomal complications.ResultsIn total, there were 16 births from 13 mothers with stomas. Of 10 births to IBD patients, 40% experienced a serious stomal complication. Caesarean section (CS) rate was 90% for IBD and 83% for non‐IBD. In‐vitro fertilisation rates were 40% in IBD patients and 0% in non‐IBD patients. The average gestational age at delivery was 36 weeks in IBD and 35 weeks in non‐IBD patients. Neonates delivered to IBD mothers had a birth weight under 2500g in 40% of cases and in non IBD mothers at 33.3% (p = 0.62). Of the sixteen births there was five complications (31.25%) associated with the stoma either during pregnancy or during the sixty‐day postpartum period.ConclusionPregnancy in stoma patients is a rare occurrence and appears to be associated with high rates of CS, preterm delivery, low birth weight and stomal complication.
Women's use of Swedish health care during the postpartum period in relation to maternal country of birth—A population‐based study
Authors: Berbres Malin , Hesselman Susanne , Ternström Elin , Schytt Erica 
Published: August 2024

AbstractIntroductionMigrant women are a heterogenous group with both higher and lower risk for pregnancy complications and adverse birth outcomes compared with women in the receiving countries. This study aimed to investigate women's use of Swedish healthcare postpartum, in terms of hospital stay >48 h, readmission to hospital, and specialized out‐patient clinic visits, in relation to maternal country of birth.Material and MethodsA population‐based register study including 278 219 primiparous and 367 776 multiparous women in Sweden (2014–2019) using data from Swedish Pregnancy Register, National Patient Register and Statistics Sweden. Multivariable logistic regression analyses were used to estimate associations between maternal country of birth and outcomes, adjusting for year of birth, maternal age, education, pre‐gestational hypertension and diabetes, and healthcare region, presented as crude and adjusted odds ratios (aOR) with 95% confidence interval (CI) with Swedish‐born women as reference.ResultsSubgroups of migrant women had higher odds of postpartum hospital stays > 48 h, particularly women from Eritrea (primiparous aOR 2.80, CI 2.49–3.15; multiparous aOR 2.78, CI 2.59–2.98), Somalia (primiparous aOR 2.61, CI 2.34–2.92; multiparous aOR 1.87, CI 1.79–1.97), and India (primiparous aOR 2.52, CI 2.14–2.97; multiparous aOR 2.61, CI 2.33–2.93), compared to Swedish‐born women. Primiparous women from Afghanistan (aOR 1.32, CI 1.08–1.6), Iraq (aOR 1.30, CI 1.16–1.46), and Iran (aOR 1.23, CI 1.04–1.45) had slightly higher odds of hospital readmission, along with multiparous women from India (aOR 1.34, CI 1.02–1.76) and Somalia (aOR 1.24, CI 1.11–1.38). Specialized out‐patient clinic visits were most common in primiparous women from Somalia (aOR 1.47, CI 1.35–1.59), Iran (aOR 1.31, CI 1.22–1.42) and Afghanistan (aOR 1.31, CI 1.18–1.46), and in multiparous women from Iran (aOR 1.30, CI 1.20–1.41) and Iraq (aOR 1.15, CI 1.11–1.20), however less common in women from some other countries.ConclusionsThe use of Swedish health care during the postpartum period varied among women, depending on their country of birth. Women from certain countries had particularly high odds of postpartum hospital stays exceeding 48 h, compared to Swedish‐born women, regardless of parity and pre‐gestational medical disorders. Further studies are needed to determine whether the individual needs of migrant women are being met during the postpartum period or not.
Cardiometabolic criteria as predictors and treatment targets of liver‐related events and cardiovascular events in metabolic dysfunction‐associated steatotic liver disease
Authors: Tamaki Nobuharu , Kimura Takefumi , Wakabayashi Shun‐Ichi , Umemura Takeji , Izumi Namiki , Loomba Rohit , Kurosaki Masayuki 
Published: August 2024

SummaryBackgroundThe diagnosis of metabolic dysfunction‐associated steatotic liver disease (MASLD) requires at least one of five cardiometabolic criteria. It is unclear whether these criteria can be used as predictors and treatment targets for complications including liver‐related events and major adverse cardiovascular events (MACE).AimsTo investigate the relationship between cardiometabolic criteria and complications.MethodsWe conducted a nationwide, population‐based study of 979,352 patients with MASLD. We investigated relationships between a number of criteria at baseline and liver‐related events or MACE risks. In a separate longitudinal analysis, we included patients with five criteria at baseline and investigated the relationship between improving the criteria and the incidence of complications after 1 year.ResultsThe cumulative incidence of MACE, but not liver‐related events, increased with increasing baseline cardiometabolic criteria. In the longitudinal study, multivariable analysis using patients with five criteria (no improvement) as the reference, adjusted hazard ratios (95% confidence interval) of MACE in patients with 4, 3, 2, and 0–1 criteria (1 to 4–5 criteria improvement) were 0.55 (0.52–0.58, p < 0.001), 0.20 (0.17–0.22, p < 0.001), 0.13 (0.11–0.16, p < 0.001), and 0.06 (0.02–0.3, p < 0.001), respectively. The risk of MACE decreased as the cardiometabolic criteria improved. There was no significant association between improvement of the criteria and liver‐related events.ConclusionsCardiometabolic criteria can be used as predictors and treatment targets for cardiovascular event risk in MASLD. Developing predictors and therapeutic targets for liver‐related events is a future challenge.
Model‐based comparisons of post‐treatment free IgE and FEV1 between omalizumab asthma dosing tables in the United States and European Union
Authors: Zhu Rui , Schoemaker Rik , Gautier Aurelie , Tian Xianbin , Sim Cheryl HooiMing , Omachi Theodore A. , Joshi Amita , Jin Jin , Owen Ryan 
Published: August 2024

AimsOmalizumab is an anti‐immunoglobulin E (IgE) monoclonal antibody that was first approved by the United States (US) Food and Drug Administration (FDA) for the treatment of allergic asthma in 2003. The pivotal trials supporting the initial approval of omalizumab used dosing determined by patient's baseline IgE and body weight, with the goal of reducing the mean free IgE level to approximately 25 ng/mL or less. While the underlying parameters supporting the dosing table remained the same, subsequent studies and analyses have resulted in approved alternative versions of the dosing table, including the European Union (EU) asthma dosing table, which differs in weight bands and maximum allowable baseline IgE and omalizumab dose. In this study, we leveraged modelling and simulation approaches to predict and compare the free IgE reduction and forced expiratory volume in 1 second (FEV1) improvement with omalizumab dosing based on the US and EU asthma dosing tables.MethodsPreviously established population pharmacokinetic‐IgE and IgE‐FEV1 models were used to predict and compare post‐treatment free IgE and FEV1 based on the US and EU dosing tables. Clinical trial simulations (with virtual asthma populations) and Monte Carlo simulations were performed to provide both breadth and depth in the comparisons.ResultsThe US and EU asthma dosing tables were predicted to result in generally comparable free IgE suppression and FEV1 improvement.ConclusionsDespite the similar free IgE and FEV1 outcomes from simulations, this has not been clinically validated with respect to the registrational endpoint of reduction in annualized asthma exacerbations.
Single gene mutations and prognosis in limited‐stage follicular lymphoma treated with radiation therapy
Authors: Hershenfeld Samantha A. , Tobin Josuha W.D. , Shelton Victoria , Calvente Lourdes , Lajkosz Katherine , Liu Ting , Brodtkorb Marianne , d'Amore Francesco Annibale , Ludvigsen Maja , Baetz Tara , LeBrun David , Johnson Nathalie , Crump Michael , Hong Michael , Kuruvilla John , Tremblay‐LeMay Rosemarie , MacManus Michael , Tsang Richard , Hodgson David C. , Gandhi Maher K. , Kridel Robert 
Published: August 2024

SummaryRadiotherapy is routinely used for management of limited‐stage follicular lymphoma (FL), yet half of patients ultimately relapse. We hypothesized that the presence of specific gene mutations may predict outcomes. We performed targeted sequencing of a 69‐gene panel in 117 limited‐stage FL patients treated with radiotherapy and identified recurrently mutated genes. CREBBP was most frequently mutated, and mutated CREBBP was associated with inferior progression‐free survival, though not after false discovery rate adjustment. This association failed to validate in an independent cohort. We conclude that recurrent gene mutations do not predict outcomes in this setting. Alternative biomarkers may offer better prognostic insight.
Staff Perspectives: Why are adolescent inpatients secluded and what causes changes in rates of seclusion on a unit?
Authors: Yurtbasi Miriam K. , Gordon Michael , Pavlou Christine , Melvin Glenn 
Published: August 2024

BackgroundThe use of seclusion in Adolescent Inpatient Psychiatric Units is being heavily scrutinised due to its forceful nature and potential to cause harm. This study aimed to understand staff perspectives on reasons for seclusion in an Adolescent Inpatient Psychiatric Unit.MethodA mixed methods approach that included the Attitudes to Seclusion Survey followed by a semi‐structured interview on the reasons for seclusion was used. We recruited 31 participants who worked on the unit of which 27 were involved in seclusion.ResultsThe findings showed that the majority of staff view seclusion as undesirable and believe it should only be used out of necessity. However, there was disagreement among staff about the reasons why adolescents were secluded and whether those reasons were justified. Staff identified factors that contributed to increases in seclusion but also provided several ways in which seclusion could be reduced.ConclusionsThese findings highlight a need for precision on what constitutes justifiable use of seclusion to reduce the potential for misuse. It also shows opportunities exist for further reduction in seclusion through teamwork and communication.
Minimum Fascia‐Tumour Distance for Differentiating Deep Lobe From Superficial Lobe Benign Parotid Tumours: A Retrospective Study and Meta‐Analysis
Authors: Lee Yi‐Chan , Tsai Yao‐Te , Tsai Ming‐Shao , Tseng Ti‐Yung , Chang Chih‐Chen , Chang Kai‐Ping 
Published: August 2024

ABSTRACTBackgroundThis study was designed to evaluate the diagnostic efficacy of the minimum fascia‐tumour distance (MFTD) in distinguishing deep‐lobe benign parotid tumours from superficial‐lobe tumours through both an original study and a meta‐analysis.MethodsIn this study, we performed a retrospective analysis of data from 91 patients who had been diagnosed with benign parotid tumours. The MFTD values were sourced from preoperative ultrasound examinations. The locations of these tumours were confirmed through surgical findings. We assessed the diagnostic accuracy of MFTD by utilising receiver operating characteristic (ROC) curves. Additionally, we conducted a systematic review of the pertinent literature and performed a diagnostic meta‐analysis to ascertain the overall diagnostic efficacy of MFTD in identifying benign parotid tumours.ResultsPatients with tumours in the deep lobe had a significantly greater MFTD than patients with tumours in the superficial lobe. Using a cutoff value of 3.50 mm for MFTD, we found an AUC of 0.93, a sensitivity of 81.8%, and a specificity of 98.8%. Our meta‐analysis included seven studies covering a total of 1689 tumours. The pooled values for sensitivity, specificity, and diagnostic odds ratio (OR) of MFTD were 81.0%, 89.0%, and 32.2, respectively. The AUC of the summarised ROC curve of MFTD was 0.90.ConclusionThe MFTD demonstrated reliable diagnostic accuracy in identifying deep‐lobe benign parotid tumours and may be incorporated into standard evaluations before parotidectomy.
Primary Team Versus Local Recovery in Liver Transplantation in the Modern Era: A National Analysis of the United Network for Organ Sharing Database
Authors: Whitrock Jenna N. , Sisak Stephanie , Pratt Catherine G. , Delman Aaron M. , Price Adam D. , Wima Koffi , Shah Shimul A. , Quillin Ralph Cutler 
Published: August 2024

ABSTRACTBackgroundThe implementation of acuity circles (AC) in 2020 and the COVID‐19 pandemic increased the use of local surgeons to recover livers for transplant; however, the impact on liver transplant (LT) outcomes is unknown.MethodsDeceased donor adult LT recipients from the UNOS database were identified.  Recipients were grouped by donor surgeon: local versus primary recovery.  Patient and graft survival as well as trends in local recovery in the 2 years pre‐AC and post‐AC were assessed.ResultsThe utilization of local recovery in LT increased from 22.3% to 37.9% post‐AC (p < 0.01).  LTs with local recovery had longer cold ischemia times (6.5 h [5.4–7.8] vs. 5.3 h [4.4–6.5], p < 0.01) and traveled further (210 miles [89–373] vs. 73 miles [11–196], p < 0.01) than those using primary recovery. Multivariate analyses revealed no differences in patient or graft survival between local and primary recovery, and between OPO and local surgeon. There was no difference in survival when comparing simultaneous liver–kidney, donation after circulatory death, MELD ≥ 30, or redo‐LT by recovery team.  Recovery and utilization rates were also noted to be higher post‐AC (51.4% vs. 48.6% pre‐AC, p < 0.01) as well as when OPO surgeons recovered the allografts (72.5% vs. 66.0%, p < 0.01).ConclusionNearly 40% of LTs are performed using local recovery, and utilization rates and trends continue to change with changing organ‐sharing paradigms such as AC.  This practice appears safe with outcomes similar to recovery by the primary team in appropriately selected recipients and may lead to increased access and the ability to transplant more livers.
Contemporary Immunosuppression Management and 1‐Year Outcomes in Dual Organ Heart Transplantation
Authors: Huang Xinyi , Salerno David , Kovac Danielle , Scheffert Jenna , Hedvat Jessica , Carver Bayleigh , Choe Jason , Shertel Tara , Yuzefpolskaya Melana , Colombo Paolo C. , Jennings Douglas L. 
Published: August 2024

ABSTRACTBackgroundThere have been limited reports on immunosuppression strategies and outcomes in dual organ heart transplant populations, primarily from before the 2018 United Network for Organ Sharing (UNOS) heart allocation policy change. Recent data suggested that outcomes with heart–lung and heart–liver transplants remained comparable in the new allocation era, yet heart–kidney recipients have worse 1‐year survival.MethodsThis single‐center retrospective study evaluated adult heart–kidney, heart–liver, and heart–lung transplant recipients from September 2019 to May 2023. Immunosuppression regimen, infectious complications, and graft outcomes were collected for 12 months.ResultsA total of 36 patients (kidney n = 20, liver n = 9, and lung n = 7) were included in this study. Basiliximab was the most commonly employed induction strategy across the organ groups (12/20 in kidney, 4/9 in liver, and 7/7 in lung). All patients were on triple immunosuppression at 12 months posttransplant with prednisone wean achieved in one heart–liver recipient. Infection complications were frequently reported (95% kidney, 75% liver, 100% lung group). One patient went back to dialysis due to focal segmental glomerulosclerosis. One chronic lung allograft dysfunction was reported, but no other severe biopsy‐proven rejection or retransplant was reported. The 1‐year survival was 85% (17/20) in heart–kidney, 78% (7/9) in heart–liver, and 86% (6/7) in heart–lung recipients.ConclusionThis study summarized real‐world immunosuppression strategies and outcomes in dual organ heart transplant recipients.