bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deep Infiltrating Endometriosis Treatment with Dienogest: A Pilot Study
Authors: Yela Daniela Angerame , Kajikawa Patricia , Donati Laura , Cursino Kleber , Giraldo Helena , Benetti-Pinto Cristina Laguna 
Published: April 2015

Background The aim of this study was to evaluate treatment with dienogest in women with deep infiltrating endometriosis (DIE). Methods A prospective cohort study was conducted at the Department of Gynecology and Obstetrics in the University of Campinas (UNICAMP), Brazil. The study included 16 women with DIE and pain which was not responsive to treatment with other progestins, who were treated with dienogest, and who attend a tertiary university hospital in 2013. Scores for pain complaints, quality of life, sexuality and extent of endometriotic lesions were evaluated before and 6 months after treatment. Statistical methods included the Wilcoxon matched-pairs test, which was used for the analysis of the quantitative data, and the relationship between them was tested by the Spearman correlation index. Results Women were a mean 36 ± 6.2 years old and reported onset of painful symptoms at a mean 29.9 ± 8.6 years of age. A significant reduction in dysmenorrhea, acyclic pelvic pain, dyspareunia and defecation pain was reported after treatment. There was no significant change in the assessment of quality of life or the Female Sexual Function Index. Intestinal lesions, which had an initial 3.4 ± 4.2 cc average size, decreased to 1.6 ± 1.8 cc, but this reduction was not statistically significant. The most common side effects were headaches and acne. Conclusions Dienogest can be used for clinical pain control in DIE.
Evaluating CA125 and VAS Pain Modifications following Gnrh Analog to Exclude Superficial Endometriosis as Cause of Chronic Pelvic Pain
Authors: Venturella Roberta , Sacchinelli Angela , Rania Erika , Lico Daniela , Di Cello Annalisa , Rocca Morena Luigia , Mocciaro Rita , Zullo Fulvio , Morelli Michele 
Published: April 2015

Background The study aim was to assess the reliability in the diagnosis of superficial endometriosis of the combined evaluation of 2 parameters: modifications of serum CA125 and VAS pain score following 1 dose of GnRH analog (GnRH-a). Methods Women with chronic pelvic pain (CPP) were managed by GnRH-a administration and diagnostic laparoscopy. Serum CA125 and VAS pain score were assessed for each patient at the early follicular phase and 2 months after the administration of 11.25 mg GnRH-a. Following laparoscopy, subjects were grouped into groups A (72 women with endometriosis) and B (46 women without endometriosis). A multivariate model for CA125 and VAS reduction and for the combination of the 2 markers was calculated using logistic regression and diagnostic performance was evaluated as the AUC of ROC curve. Main outcome measure was the accuracy of the modifications of serum CA125 levels and VAS score following GnRH-a, in patients with histological diagnosis of superficial endometriosis. Results At baseline, both groups showed similar CA125 levels and VAS scores. Two months after GnRH-a, a significant reduction (delta [Δ]) in CA125 levels and VAS pain score was observed in group A only. AUCs for ΔCA125, ΔVAS score and for combination of these 2 deltas were 0.90, 0.83 and 0.97, respectively. Conclusions The assessment of serum CA125 and VAS pain score following GnRH-a demonstrates good reliability to exclude superficial endometriosis in patients with CPP. The response to GnRH-a administration in these women could therefore be employed as an ex juvantibus criterion for endometriosis diagnosis.
Association between Let-7 microRNA-Binding-Site Polymorphism in the KRAS 3'UTR and Endometriosis: A Replication Study and Meta-Analysis
Authors: Pagliardini Luca , Vanni Valeria S. , Papaleo Enrico , Corti Laura , Candiani Massimo , Vigano’ Paola 
Published: April 2015

Background Endometriosis is inherited as a complex genetic trait, and the single nucleotide polymorphism (SNP) rs61764370 within the KRAS gene on chromosome subband 12p12.1 has been proposed as a potential candidate gene. By disrupting a binding site for microinterference RNA (miRNA) let-7, the rs61764370 SNP variation site may increase activation of KRAS and favor disease development. Conflicting evidence, however, has emerged on the association between the rs61764370 SNP and endometriosis. Due to the potential implications of this issue for the diagnosis and treatment of endometriosis, we sought to replicate the sequencing of the KRAS rs61764370 SNP in a population of cases and controls and to perform a meta-analysis encompassing all currently available studies as well as our novel replication. Methods We sought to replicate for the first time the sequencing of KRAS rs61764370 SNP in a highly selected population of 86 cases of women with laparoscopically proven endometriosis and 72 healthy controls, and to perform a meta-analysis encompassing currently available studies and including affected subjects (n = 2,225) and controls (n = 1,923). Results The rs61764370 minor allele was observed in 12 of 86 women with endometriosis (14.0%) and in 15 of 72 controls (20.8%) (odds ratio [OR] = 0.76, 95% confidence interval [95% CI], 0.36-1.60, p = 0.48). The meta-analysis failed to identify any significant association (OR = 1.03, 95% CI, 0.89-1.20, p = 0.67, phet = 0.46). Conclusions KRAS variation site rs61764370 is unlikely related to disease development, and other loci with potential implications for diagnosis or treatment for endometriosis should be identified.
HISTIOCITOSE DE CÉLULAS DE LANGHERHANS. RELATO DE CASO
Authors: Bacarin João Vitor , Velho Pedro Henrique Isaacson , Slovinski Augusto Passoni , Frandoloso Gibran Avelino , Carvalho Maurício 
Published: April 2015

Introdução: As células de Langerhans são encontradas na epiderme, nas células interdigitais dos linfonodos, no timo, no epitélio mucoso gastrointestinal e respiratório e na cérvice; mantendo função de células apresentadoras de antígenos. A Histiocitose de células de Langerhans é caracterizada por uma proliferação anormal de histiócitos. A doença pode ser localizada ou disseminada, envolvendo dois ou mais sistemas. A Histiocitose das células de Langerhans pulmonar é uma condição rara que acomete principalmente homens jovens e tabagistas. Relato: Paciente do sexo masculino, 27 anos, branco, trabalhador da construção civil, apresentou há dois anos dispnéia que evoluiu com piora progressiva. Há seis meses evoluiu com tosse seca, febre, sudorese noturna, dor torácica ventilatório-dependente, ganho de peso de cerca de 10 kg. A tomografia computadorizada de tórax demonstrou padrão de múltiplos nódulos e cistos que poupavam os seios costofrênicos. O lavado bronco alveolar revelou predomínio de macrófagos. Teste de restrição hídrica positivo para diabetes insipidus de origem central. O paciente foi diagnosticado com a forma pulmonar da histiocitose de células de Langerhans associado a diabetes insipidus central. Conclusão: A Histiocitose de células de Langerhans é uma doença rara que pode ter acometimento de múltiplos sistemas. Na presença de manifestação clínica em um sítio deve-se proceder a investigação de outros possíveis focos da doença.
DO EDITOR
Authors: Carvalho Maurício 
Published: April 2015

“O papel das resinas trocadoras de íons na hipercalemia aguda com manifestação eletrocardiográfica é questionável, pois o tempo necessário para a ação da resina é incompatível com a rapidez necessária nesta situação que ameaça à vida”. Esta é a conclusão do artigo de revisão da Professora Maria Aparecida Pachaly, da Disciplina de Nefrologia da UFPR, publicado nesta edição da RMU. A hipercalemia, definida como potássio sérico acima de 5,5 mEq/L, constitui emergência clínica, que demanda rápido tratamento pelo médico atendente. Nesta revisão, a Dra. Maria Aparecida aborda de maneira crítica os tipos de resina disponíveis, seus benefícios e limitações. Leitura imperdível.Dois artigos muito interessantes, resultado de palestras proferidas durante a Semana de Comemoração dos 100 anos da faculdade de Medicina da UFPR, também fazem parte desta edição. O professor Miguel Ibraim A. Hanna Sobrinho, chefe do DCM, aborda o tema Formação Médica e a Evolução do Curso de Medicina. E a chefe do Setor de Ciências da Saúde, professora Claudete Reggiani, discorre sobre o passado, presente e futuro do curso de Medicina da UFPR. Boa Leitura, Mauricio de Carvalho Editor
RESINAS TROCADORAS DE CÁTIONS NA HIPERCALEMIA AGUDA GRAVE
Authors: Pachaly Maria Aparecida 
Published: April 2015

As resinas trocadoras de cátions fazem parte da abordagem terapêutica tradicional da hipercalemia. As resinas de troca catiônica são substâncias sintéticas, não absorvíveis, que liberam um cátion que faz parte de sua composição e captam outro cátion de interesse, no caso, o potássio, causando a redução da calemia. As resinas disponíveis atualmente são: poliestireno sulfonato de sódio (PSS), nos Estados Unidos, e poliestireno sulfonato de cálcio (PSC), no Brasil. Grande parte da literatura encontrada sobre estas resinas é proveniente de países onde é utilizado o PSS, cuja sua estrutura química e efeitos são similares aos do PSC.Porém, como as resinas atualmente disponíveis são efetivas a partir de 24 horas de uso, não atendem à demanda pela remoção rápida de potássio, como ocorre na hipercalemia aguda com toxicidade cardíaca e manifestações eletrocardiográficas. Além disto, apesar de não serem absorvidas, as resinas não são substâncias inócuas, e podem causar complicações graves no aparelho digestivo.Decisões sobre o uso das resinas na hipercalemia aguda grave devem ser feitas com cautela e considerando o contexto de cada paciente. O objetivo desta revisão é trazer mais dados que permitam ao leitor uma visão crítica sobre o papel real destas substâncias no tratamento da hipercalemia aguda.
OPINIÕES DOS PROFISSIONAIS DE TERAPIA INTENSIVA SOBRE OS PROCEDIMENTOS DE REALIMENTAÇÃO ORAL EM PACIENTES NEUROCRÍTICOS
Authors: Felicetti-Lordain Cláudia R. , Tozetto Altevir J. G. , Duarte Silvana T. , Furkim Ana Maria , Duarte Péricles A. D. 
Published: April 2015

Procurou-se verificar o procedimento que profissionais e estudantes que atuam em unidade de terapia intensiva (UTI) adotam para realimentação oral em pacientes neurológicos agudos após saída da ventilação mecânica, através de um questionário aplicado a profissionais de saúde (médicos intensivistas, neurologistas e enfermagem da UTI), residentes médicos e estudantes de medicina em um hospital público de ensino. O questionário continha questões sobre critérios de consistência de alimentos e questões para escolher a melhor dieta oral para pacientes com doenças neurológicas agudas. A pesquisa foi respondida por 14 médicos, 9 residentes, 13 estudantes de medicina e 21 profissionais de enfermagem. Foi feita estatística descritiva e comparação entre as médias, com análise univariada. Os parâmetros mais citados para indicar realimentação oral foram: adequado nível de consciência, eficiência da deglutição, e estabilidade gastrointestinal. As consistências de alimentos mais frequentemente citadas para realimentação oral foram pastosos e semipastosos. Contudo, houve várias respostas contraditórias sobre consistência de alimentos, particularmente sobre líquidos. Vários profissionais e estudantes demonstraram pouca preocupação sobre aspectos de disfagia nestes pacientes. Não há concordância sobre procedimentos de realimentação oral no paciente neurocrítico agudo entre profissionais atuantes em terapia intensiva, principalmente sobre selecionar a consistência de alimento a ser testada e no reconhecimento da importância da disfagia neste grupo.
TERIA F. CHOPIN SOFRIDO DE FIBROSE CÍSTICA? REVISÃO HISTÓRICA
Authors: Pedro Matheus Kahakura Franco 
Published: April 2015

Introdução: o compositor polonês Fréderic Chopin (1810-1849) sofreu toda vida com uma afecção pulmonar, na época diagnoticada tuberculose; fibrose cística é um diagnóstico provável. Objetivo e Métodos: descrever a doença de Chopin como relato de caso. Revisados 5 artigos sobre sua doença, em inglês, na base de dados PubMed. Resultados: desde a infância Chopin sofreu de diarréia recorrente, perda de peso, dispnéia e infecções respiratórias. Em 1838 desenvolveu tosse, febre e hemoptise. O diagnóstico foi tuberculose, nunca confirmado pela sua ausculta pulmonar; ainda, nenhum contato de Chopin adquiriu a doença. Nos próximos anos, teve tosse produtiva, dispnéia progressiva e cianose. Em 1849 sofreu três dias com tosse intensa e dispnéia ao repouso, falecendo em 17/10. À autópsia, cardiomegalia foi a única alteração descrita (possível cor pulmonale). Sua história familiar e esterilidade também sugerem fibrose cística. Conclusão: a doença de Chopin fez parte das tragédias que o inspiraram; embora os médicos da época tenham diagnosticado tuberculose, o conhecimento médico atual permite desenvolver outras hipóteses, sendo a fibrose cística extremamente plausível.
DNA Damage Response in Neonatal and Adult Stromal Cells Compared With Induced Pluripotent Stem Cells
Authors: Liedtke Stefanie , Biebernick Sophie , Radke Teja Falk , Stapelkamp Daniela , Coenen Carolin , Zaehres Holm , Fritz Gerhard , Kogler Gesine 
Published: April 2015

Abstract

Comprehensive analyses comparing individual DNA damage response (DDR) of induced pluripotent stem cells (iPSCs) with neonatal stromal cells with respect to their developmental age are limited. The imperative necessity of providing developmental age-matched cell sources for meaningful toxicological drug safety assessments in replacement of animal-based testing strategies is evident. Here, DDR after radiation or treatment with N-methyl-N-nitrosurea (MNU) was determined in iPSCs compared with neonatal and bone marrow stromal cells. Neonatal and adult stromal cells showed no significant morphologically detectable cytotoxicity following treatment with 1 Gy or 1 mM MNU, whereas iPSCs revealed a much higher sensitivity. Foci analyses revealed an effective DNA repair in stromal cell types and iPSCs, as reflected by a rapid formation and disappearance of phosphorylated ATM and γH2AX foci. Furthermore, quantitative polymerase chain reaction analyses revealed the highest basic expression level of DDR and repair-associated genes in iPSCs, followed by neonatal stromal cells and adult stromal cells with the lowest expression levels. In addition, the influence of genotoxic stress prior to and during osteogenic differentiation of neonatal and adult stromal cells was analyzed applying common differentiation procedures. Experiments presented here suggest a developmental age-dependent basic expression level of genes involved in the processing of DNA damage. In addition a differentiation-dependent downregulation of repair genes was observed during osteogenesis. These results strongly support the requirement to provide adequate cell sources for toxicological in vitro drug testing strategies that match to the developmental age and differentiation status of the presumptive target cell of interest.

Significance
The results obtained in this study advance the understanding of DNA damage processing in human neonatal stromal cells as compared with adult stromal cells and induced pluripotent stem cells (iPSCs). The data suggest developmental age-dependent differences in DNA damage repair capacity. In iPSCs (closest to embryonic stem cells), the highest expression level of DNA damage response and repair genes was found, followed by neonatal stromal cells and adult stromal cells with the lowest overall expression. In addition, a differentiation-dependent downregulation of repair capacity was observed during osteogenic differentiation in neonatal stromal cells. Notably, the impact of genotoxic stress on osteogenic differentiation depended on the time the genotoxic insult took place and, moreover, was agent-specific. These results strongly support the necessity of offering and establishing adequate cell sources for informative toxicological testing matching to the developmental age and differentiation status of the respective cell of interest.
No differences in grades or level of satisfaction in a flipped classroom for neuroanatomy
Authors: Whillier Stephney , Lystad Reidar Petter 
Published: April 2015

ObjectiveThe intensive nature of a 5- or 6-week teaching block poses unique problems for adequate delivery of content. This study was designed to compare the delivery of a unit of undergraduate neuroanatomy in a short summer school period, as a traditionally taught unit, with a rendition given in the form of the “Flipped Classroom.” The aim was to evaluate the effectiveness of the flipped classroom in the intensive mode classroom.MethodsThe flipped classroom encompassed the same learning outcomes, but students were responsible for covering the content at home in preparation for tutorials that applied their acquired knowledge to higher levels of thinking. The main outcome measures were the final course grades and the level of satisfaction with the course.ResultsThere were no significant differences between the 2 cohorts in final grades (p = .259), self-rated knowledge (p = .182), or overall satisfaction with the course (p = .892).ConclusionThis particular design of the flipped classroom did not add value to the intensive mode experience. It may be that this mode of delivery is ill suited to intensive classes for subjects that carry a lot of content. The use of the flipped classroom requires further research to fully evaluate its value.
Delocalized Deformation Enhanced Reusable Energy Absorption Metamaterials Based on Bistable Tensegrity
Authors: Yang Hao , Zhang Jie , Wang Ji , Hu Jinbo , Wu Zhigang , Pan Fei , Wu Jianing 
Published: August 2024

AbstractMechanical metamaterials are rationally designed structures possessing exceptional properties that can be manufactured by 3D printing techniques. Mechanical metamaterials provide an unprecedented platform for energy absorption, mitigating damage caused by severe localized impacts within confined areas. However, current designs always reveal deficiencies either in their energy absorption capacities or in their suitability for repetitive utilization. To address such limits, a novel bistable tensegrity structure with superior reusability is derived from a classical tensegrity structure, and a tensegrity‐based assembly strategy is proposed to construct these bistable structures into mechanical metamaterials with a delocalized deformation mechanism. Upon a localized impact on a single loading node, all the elastic components of each reusable bistable structure in the mechanical metamaterials stretch synchronously for energy absorption, exhibiting higher energy‐absorbing capacity. Here, the metamaterials achieve an energy‐absorbing capacity of 26.4 kJ (kg m2)−1 over 10 000 cycles, outperforming other reusable materials by ≈2 orders of magnitude in energy‐absorbing capacity and reusability, respectively. This study provides a novel tensegrity‐based design assembly strategy for developing high‐capacity, reusable energy‐absorbing materials that are suitable for advanced impact protection and engineering systems.
Near‐Field‐Regulated Ultrafast Laser Supra‐Wavelength Structuring Directly on Ultrahard Metallic Glasses
Authors: Huang Hanxuan , Song Shijie , Liu Yun , Liu Zhenyu , Xiao Zifeng , Li Yanyang , Wang Yi , Li Ruifan , Zhao Qianru , Wang Xudong , Chen Yu , Wang Lei , Hou Zhishan , Zhang Peilei , Sun Yun‐Lu 
Published: August 2024

AbstractThe ultrafast‐laser‐matter interactions enable “top‐down” laser surface structuring, especially for materials difficult to process, with “bottom‐up” self‐organizing features. The subwavelength scenarios of laser‐induced structuring are improved in defects and long‐range order by applying positive/negative feedbacks. It is still hardly reported for supra‐wavelength laser structuring more associated with complicated thermo/hydro‐dynamics. For the first time to the knowledge, the near‐field‐regulated ultrafast‐laser lithography of self‐arrayed supra‐wavelength micro/nano‐pores directly on ultra‐hard metallic glass is developed here. The plasmonic hot spots on pre‐structures, as the positive feedback, clamped the lateral geometries (i.e., position, size). Simultaneously, it drilled and self‐organized into micro/nano‐pore arrays by photo‐dynamic plasma ablation and Marangoni removal confined under specific femtosecond‐laser irradiation, as the negative feedback. The mechanisms and finite element modeling of the multi‐physical transduction (based on the two‐temperature model), the far‐field/near‐field coupling, and the polarization dependence during laser‐matter interactions are studied. Large‐area micro/nano‐pore arrays (centimeter scale or larger) are manufactured with tunable periods (1–5 µm) and geometries (e.g., diameters of 500 nm–6 µm using 343, 515, and 1030 lasers, respectively). Consequently, the mid/far‐infrared reflectivity at 2.5–6.5 µm iss decreased from ≈80% to ≈5%. The universality of multi‐physical coupling and near‐field enhancements makes this approach widely applicable, or even irreplaceable, in various applications.
Electrochemical Approach Toward Mesoionic 1,2,3‐Triazole 1‐Imines
Authors: Shuvaev Alexander D. , Feoktistov Matvey A. , Teslenko Fedor E. , Fershtat Leonid L. 
Published: August 2024

AbstractSynthetic electrochemistry may establish direct routes to a preparation of a plethora of organic substances, which are hardly accessible by conventional experimental techniques. Herein, we present an electrochemically‐driven method for an assembly of a broad range of rare heterocyclic mesoionic entities –1,2,3‐triazole 1‐imines. These nitrogen heterocycles were prepared through a transition‐metal‐ and exogenous oxidant‐free strategy using a C/Ni electrode pair. Over 30 examples of thus synthesized 1,2,3‐triazole 1‐imines illustrate selectivity and practical utility of this approach. Key solvent‐controlled reactivity patterns for the formation of the triazole imine scaffold were revealed indicating a modulation ability of the developed approach. These experimental findings were additionally justified based on cyclic voltammetry (CV) data and density functional theory (DFT) calculations. Moreover, according to differential scanning calorimetry (DSC) data, some of the prepared 1,2,3‐triazole 1‐imines correspond to the thermally stable species with an onset decomposition temperature up to 190 °C.
Clinical features and risk factors for delayed rupture of traumatic cerebral aneurysm: A case series
Authors: Sugii Masataka , Okada Kazuhiro , Ikeda Shimpei , Hara Yoshiaki , Yokobori Shoji 
Published: August 2024

AbstractBackgroundTraumatic cerebral aneurysms (TA) are a subset of traumatic cerebrovascular injury (TCVI). Misdiagnosis of TA can be fatal. To investigate factors that predict TA formation and the optimal timing for searching, we present four suspected cases of delayed TA rupture during hospitalization.Case PresentationMedical records of head injury cases to have delayed TA rupture during hospitalization between April 2021 and March 2022 were retrospectively reviewed. Of the four patients included, only one met the TCVI screening criteria. All the patients had acute subdural hematoma (ASDH) on arrival; two had delayed expansion of the traumatic subarachnoid hemorrhage (tSAH) on repeat imaging. All the patients received anticoagulants. Ruptured TA occurred between days 5 and 11. Three patients died during hospitalization.ConclusionIt is advisable to suspect TA when imaging studies show ASDH on admission and intracranial hematoma expansion during hospitalization. We suggest TA screening around day 5.