bluuebunny/crossref_metadata_2025_split huggingface dataset:
Strong Anti‐tumor Effect of Monosialoganglioside Specific Monoclonal Antibody 202: A Clinical Trial in a Cancer Patient with Melanoma
Authors: Hamanaka Sumiko , Ota Takahisa , Nakayasu Kiyoshi , Kamei Toshiaki , Tai Tadashi , Kawashima Ikuo , Otsuka Fujio , Irie Reiko , Asagami Chidori 
Published: April 2015

AbstractMab 202, a mouse monoclonal IgM antibody which recognizes sialic acid α2→3 galactosyl residue in monosialogangliosides and reacts with human melanoma cells but not with normal cells, was administered to a melanoma patient by either intralesional injection or intravenous infusion. Mab 202 induced regressions of the metastatic tumors without side effects. Histopathologic examination showed remarkable degenerative and necrotic changes in the tumor, around which lymphocytes, eosinophils, plasma cells and macrophages infiltrated. Immuno‐peroxidase staining revealed Mab 202 binding to melanoma cells. Clinical and pathologic evidence suggested that Mab 202 has cytotoxic effects against melanoma cells. Mab 202 may therefore be useful in the treatment of human malignant melanoma.
Parallel Arrangement, Growth Inhibition and Cell Cycle Phase Analysis of Human Dermal Fibroblasts Cultured in Collagen Lattice
Authors: Kono Takeshi , Tanii Tsukasa , Furukawa Masayoshi , Mizuno Nobuyuki , Kitajima Jun‐ichi , Ishii Masamitsu , Hamada Toshio , Yoshizato Katsutoshi 
Published: April 2015

AbstractHuman dermal fibroblasts were cultured in a hydrated type I collagen lattice. When collagen fibers were arranged in one direction, fibroblasts were arranged in the same direction. Cell proliferation was markedly suppressed in the collagen lattice as compared with that on plastic, with growth being arrested after day 5. No differences in proliferation were observed between aligned cells and randomly oriented cells.Flow cytometry with DNA staining was performed to analyze each phase of the cell cycle of fibroblasts. Among the 10,000 cell population, S phase cells on day 2 of culture accounted for 43% on plastic but were markedly inhibited to 25% in the lattice. On day 4, S phase cells accounted for 33% on plastic but only for 10% in the lattice. These findings suggest that cell advancement to the S phase is markedly inhibited in the collagen lattice, resulting in accumulation of most of cells in the G0G1 phase.The present study clearly showed that culture in the collagen lattice allowed alignment of fibroblasts with a definite orientation as observed in vivo and produced a status resembling that in vivo in terms of proliferation and cell cycle phase composition.
A New Method for Culturing Human Hair Follicle Cells on Floating Mixed Collagen Membranes
Authors: Kuwana Ryuichiro , Arase Seiji , Sadamoto Yasushi , Takeda Katsuyuki 
Published: April 2015

AbstractA new method for culturing human hair follicle cells without a biological feeder layer has been developed.Dispersed hair follicle cells obtained from plucked hairs have been successfully grown on floating mixed collagen membranes (collagen types I and IV) and have reached a confluent state. They could be subcultured up to twice. Differentiation of the cells was maintained throughout a month in culture. Stratification of the cells and continuous epithelial pavement formation were seen, as in cultured epidermal keratinocytes.
A Study of Systemic and Topical Effects of Topical Steroid Application through the Comparison of Two Application Schedules
Authors: Fukaya Yoshihide , Masutani Mamoru , Koyama Youichi , Takahashi Hisahide , Ueda Hiroshi 
Published: April 2015

AbstractWe compared two application schedules of a topical steroid, BV (Betamethasone valerate). The schedules were once daily and twice a week on juvenile Wistar rats treated for four weeks. Steroid actions were assessed topically (skin thickness and epidermal Langerhans cell density) and systemically (body weight and internal organ wet weights). The results revealed some rather severe systemic effects caused by frequent steroid application. And we also found a uniquely weak BV effect with Langerhans cells compared to obvious systemic effects on rats. We came to the conclusion that frequent steroid application to the young should be avoided.
Radio‐active Iodine Uptake in Vitiligo
Authors: Kumar Vinay , Shankar Vijay , Chaudhary Satdev , Bhatia Krishan Kumar , Mehta Lalit Kumar , Arora Nishi , Arora Des Raj 
Published: April 2015

AbstractVitiligo and thyroid disease are commonly associated disorders. Twenty‐two clinically euthyroid vitiligo patients were studied for functional assessment of thyroid by radioactive iodine uptake assay. Half of them showed abnormal uptake values at 24 hours. Of these patients, 90% had lower values indicating a tendency towards developing hypothyroid state. Subclinical thyroid dysfunction in vitiligo appears to be an adaptive change.
Correlation of Contractility and Proliferative Potential with the Extent of Differentiation in Mouse Fibroblastic Cell Lines Cultured in Collagen Lattices
Authors: Kono Takeshi , Tanii Tsukasa , Furukawa Masayoshi , Mizuno Nobuyuki , Taniguchi Shoji , Ishii Masamitsu , Hamada Toshio , Yoshizato Katsutoshi 
Published: April 2015

AbstractFour types of fibroblastic cell lines at various stage of differentiation, which had been derived from syngeneic mice, were cultured in collagen lattices (reconstituted dermis model). Lattice contraction, growth in the lattice, and cell morphology were compared. The following cell lines were used: [I] precrisis cells within several subcultures derived from the skin of Balb/c mice, [II] an established normal cell line derived from syngeneic mice (Balb/3T3 clone A31), and [III] two transformed lines (Balb/3T12‐3, 3T3‐B‐SV40) originating from [II]. The cells adopted a bipolar spindle form in the collagen lattice. Lattice contraction was the most marked with cell type [I] followed in order by [II] and [III]. Relative growth in the lattice occurred in the reverse order (III>II>I). These findings suggested a correlation between lattice contraction and growth in the lattice and also between the extent of differentiation and lattice contraction.
Immunohistochemical Study of Lysozyme in Human Apocrine Glands
Authors: Ezoe Kazuhiko , Katsumata Michio 
Published: April 2015

AbstractThe localization of lysozyme in human apocrine glands was studied by adopting the avidin‐biotin‐peroxidase complex method. The results showed that the glands were enriched with lysozyme. The apical portion of secretory cells was most heavily stained. Eccrine glands did not stain for lysozyme. Although apocrine glands have been regarded as having no apparent function in man, it is suggested in the present report that they may have an excretory bactericidal role.
Generalized Rash Induced by Sulfhydryl Drug in Guinea Pigs
Authors: Kitamura Kazuko , Aihara Michiko , Ikezawa Zenro 
Published: April 2015

AbstractAdverse reactions produced by sulfhydryl compounds with active thiol groups have generally formed a distinctive pattern in man when they are viewed as a class. It has been reported that cutaneous SH‐induced drug eruptions have a wide variety of clinical presentations; histologically, they show a pattern of eosinophilic necrosis and/or satellite necrosis similar to that seen in cutaneous graft vs host reactions.In the present experiments, guinea pigs were sensitized with cephalothin (CET) and thiol compounds such as tiopronin (TP), D‐penicillamine, gold sodium thiomalate or thiomalate, using a method similar to that described previously. In lymphocyte stimulation tests, lymph node cells from the sensitized animals responded positively to spleen cells pulsed with each thiol compound. Intracutaneous tests revealed some positive reactions to each thiol compound; there was a tendency to produce a tuberculin type reaction with indurated erythema rather than the Jones‐Mote type seen in CET‐induced reactions. The dose‐requirements for positive intracutaneous tests and generalized rash (GR) due to thiol compounds were lower than for CET, which required relatively large doses. Histologically, infiltration of basophilic cells was prominent in the skin lesions induced by intracutaneous tests with CET and in those of CET‐induced GR. On the other hand, intracutaneous tests with TP following the induction of TP‐induced GR revealed eosinophilic degeneration of epidermal cells, which was similar to the eosinophilic necrosis seen in cutaneous GVHR. Intracutaneous tests after the induction of CET‐GR did not show any eosinophilic changes in the epidermal cells.These findings are reminiscent of the characteristics of eruptions induced by thiol compounds in man, which differ from the eruptions induced by CET.
Isolation and Culture of Panning Method‐enriched Langerhans Cells from Dispase‐dissociated Epidermal Cells of the Mouse
Authors: Koyama Yoh‐ichi , Kobayashi Miya , Ohashi Koji , Nagao Shizuko , Niwa Jun‐ichi , Takahashi Hisahide , Hoshino Takeshi , Marunouchi Tohru 
Published: April 2015

AbstractLangerhans cells (LCs) are bone marrow‐derived, Ia‐positive antigen‐presenting cells in the epidermis which constitute 2–4% of the total epidermal cells. We examined the usefulness of a combination of dispase treatment and the panning method for enriching and culturing mouse LCs. Trunk skin was treated with partially purified dispase (Godo Shusei, type II) to separate epidermal sheets and to dissociate epidermal cells. Suspended cells were treated with ascites or culture supernatant containing anti‐Ia monoclonal antibody, and LCs were enriched by the Ia‐mediated panning method. Per mouse, 3–4 × 105 LCs were recovered with >95% purity and >90% viability. Enriched LCs potently stimulated the allogeneic mixed‐leukocyte reaction. Ultrastructural observations revealed that enriched LCs contained many vesicles but almost no Birbeck granules. A laminal structure, which was apparently adhesive to the surface of LCs, was observed when ascites were employed as the anti‐Ia antibody. These results indicate that a combination of dispase treatment and the Ia‐mediated panning method is very useful for isolating high yields of functionally mature murine Langerhans cells with high purity and viability.
Ultrasonography of Proliferative Vascular Lesions of the Skin
Authors: Betti Roberto , Nessi Renato , Blanc Marco , Bencini Pier Luca , Galimberti Michela , Crosti Carlo , Uslenghi Carlo 
Published: April 2015

AbstractHigh‐resolution ultrasonography with small part probes was performed on 20 proliferative vascular lesions of the skin (3 lymphangiomas, 9 hemangiomas, 8 Kaposi sarcomas). All the lesions were submitted to histological examination. The histological cuts were made in the same planes as the ultrasound scans in order to obtain a direct comparison.The sonographic pattern of each histotype is characteristic and correlated with the corresponding histological picture. Lymphangiomas appear as large anechoic communicating cavities; hemangiomas have small hypoechoic areas separated by thin hyperechoic septa. Kaposi sarcomas possess a mixed sonographic structure with ill‐defined margins.Ultrasound is proposed as a simple and harmless diagnostic aid to clinical examination of proliferative vascular lesions of the skin.
Recurrent Male Herpes Genitalis with Two Different Strains of Herpes Simplex Virus Type 2
Authors: Yoshida Masami , Tezuka Tadashi 
Published: April 2015

AbstractA case of a 50‐year‐old man with recurrent herpes genitalis with two different isolated strains of herpes simplex virus (HSV) type 2 is reported. Morphologically, two types of cytopathic effects (CPE) induced by viruses were observed in the Vero cell cultures, one being a syncytial giant cell formation and the other a rounded cell formation without cell adhesion resembling the CPE induced by ordinary HSV type 2. In this case, it is clinically interesting that the patient complained of persistent pain on the glans and urethra without recurrence of herpetic lesions even after the involution of enanthema. The correlation between the persistent pain and neurovirulence of the two different HSV strains is discussed.
A Familial Occurrence of Sporotrichosis
Authors: Yamada Yoshitaka , Dekio Satoshi , Jidoi Joji , Ozasa Shozaburo , Tohgi Kimiko 
Published: April 2015

AbstractA grandfather and granddaughter suffering from sporotrichosis were reported. It is thought that they might have been infected from the same source of Sporothrix schenckii (S. schenckii).
Lymphatic Invasion of Nevus Cells Observed in Intradermal Nevus
Authors: Katsumata Michio , Matsunaga Tsuyoshi , Maruyama Ryuji , Ezoe Kazuhiko 
Published: April 2015

AbstractA pigmented nevus was observed on the medial aspect of the left scapula of a 30‐year‐old woman. Histologically, the lesion showed the pattern of a typical intradermal nevus, consisting of type A, B and C cells. However, the most characteristic feature of this intradermal nevus was the projection of polypoid masses of nevus cells containing melanin into the lumen of a lymphatic vessel in the upper dermis. To our knowledge, lymphatic invasion in pigmented nevi is rare; this finding is interesting, if we consider the relationship between nevus cells and lymph nodes.
Two‐dimensional Polyacrylamide Gel Electrophoresis of Human Hair Matrix Proteins Using Isoelectric Focusing Electrophoresis as the First Dimension
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractS‐carboxymethylated (SCM) matrix proteins from fifteen normal human scalp hair specimens were analyzed using two‐dimensional polyacrylamide gel electrophoresis (PAGE) with usual isoelectric focusing electrophoresis as the first dimension and then with usual sodium dodecyl sulfate‐PAGE as the second dimension. On the electrophoregrams obtained, one low‐mobility smear of SCM proteins embedding one SCM protein band was always seen. Many higher‐mobility SCM protein spots were also always seen. However, some variations were seen in these SCM protein compositions; in type I, there were eighteen SCM protein components and, in type II, seventeen components.
Depletion and Repopulation of Langerhans Cells in Nonsegmental Type Vitiligo
Authors: Kao Chao‐Hsing , Yu Hsin‐Su 
Published: April 2015

AbstractThe role of Langerhans cells in the pathogenesis of nonsegmental type vitiligo is still unknown. In this study, biopsies were taken from 26 patients at various stages of nonsegmental type vitiligo and morphometrically observed to investigate the kinetics of Langerhans cells in patients at various stages of the disease. A marked depletion of OKT6‐positive and ATPase‐positive epidermal dendritic cells was noted in patients with active nonsegmental type vitiligo. A repopulation of both OKT6‐positive and ATPase‐positive epidermal dendritic cells was noted in patients with stable nonsegmental type vitiligo. Profound depletion of epidermal OKT6‐positive and ATPase‐positive dendritic cells was noted in patients with repigmenting nonsegmental type vitiligo receiving treatments involving topical use of 0.05% Fluocinonide cream or PUVA photochemotherapy. Transmission electron microscopy confirmed the absence of epidermal dendritic cells (Langerhans cells and intermediate cells) in patients with active and repigmenting nonsegmental type vitiligo. In active nonsegmental type vitiligo, two possible explanations are proposed for the depletion of OKT6‐positive and ATPase‐positive epidermal dendritic cells (presumptive Langerhans cells): 1) the cells are destroyed by cytotoxic factors released during the course of destruction of melanocytes in active vitiligo, and/or 2) they leave the epidermis and migrate to regional lymph nodes to present certain antigens which are released from certain destroyed epidermal cells (keratinocytes or melanocytes) during the course of active vitiligo. The repopulated epidermal Langerhans cells may result from phenotypically transformed dermal dendritic cells in the depigmented lesions of patients with stable vitiligo. Since various therapies which result in repigmentation deplete the density of epidermal Langerhans cells markedly, it is suggested that depletion of epidermal Langerhans cells in stable vitiligo may aid in repigmentation. It is also proposed that the repopulated epidermal Langerhans cells may play a role in inhibiting the proliferation of epidermal melanocytes in depigmented lesions of stable vitiligo, thus various methods of treatment which deplete the Langerhans cells may eventually aid in the repigmentation of nonsegmental type vitiligo.
Antiproliferative Effect of Hyperthermia and ACNU on Cultured Human Malignant Melanoma Cells
Authors: Someya Tohru , Nogita Toshitatsu , Yamada Kiyoshi , Tsuchida Tetsuya , Watanabe Ryoji , Otsuka Fujio 
Published: April 2015

AbstractHuman malignant melanoma cultured cells were treated either with ACNU (1‐(4‐amino‐2‐methyl‐5‐pyrimidinyl)methyl‐3‐(2‐chlorethyl)‐3‐nitrosourea hydrochloride), hyperthermia, or the combination of ACNU and hyperthermia. The combination treatment inhibited the cell growth to a slightly synergistic degree compared to the respective single treatments. The present in vitro experimental results support in part the finding of our previous report that the combination treatment with ACNU and hyperthermia have a significantly synergistic antitumor effect to human melanoma transplanted to nude mice. However, the synergistic effect was much less intense in the present in vitro experiment. The difference may have resulted from the environmental differences between in vitro and in vivo experimental systems.
Cicatricial Pemphigoid with Linear IgA Deposit
Authors: Tamaki Kunihiko , Nashiro Kiyoko , Ishii Akiko 
Published: April 2015

AbstractA Japanese woman with typical clinical and histological manifestations of cicatricial pemphigoid was presented. Direct immunofluorescent (IF) investigation of perilesional skin revealed in vivo deposits of IgA but not of IgG, IgM, or C3. Indirect IF study revealed that this patient had circulating antibody against epidermal basement membrane zone of the IgA class. We would like to classify this case as cicatricial pemphigoid with IgA deposits rather than as a cicatricial variant of linear IgA bullous dermatosis.
Desferrioxamine in the Treatment of Porphyria Cutanea Tarda: Ineffectiveness of Intramuscular Administration
Authors: Muñoz Juan J. , Garcia‐Cabezas Juan , Martinez‐Verano Juan , Perez‐Oteyza Carlos , de Salamanca Rafael Enriquez 
Published: April 2015

AbstractFour patients suffering from porphyria cutanea tarda received intramuscularly 10–15 g per month of desferrioxamine for periods ranging between 21 and 50 months. Porphyrin excretion was serially analyzed during this treatment.Desferrioxamine administration did not induce remission of the disease, which was subsequently achieved after treatment either with phlebotomies or chloroquine.Therefore, chronic intramuscular administration of desferrioxamine alone is ineffective in porphyria cutanea tarda.
Malignant Histiocytosis with Panniculitis
Authors: Kullavanijaya Preya , Kulthanan Kanokvalai 
Published: April 2015

AbstractWe report a case of malignant histiocytosis which began with the skin lesions of panniculitis. A 32‐year‐old woman presented with recurrent erythematous plaques, subcutaneous nodules, and ulcers on the trunk and the extremities and intermittent fever for 7 months. The cutaneous lesions consisted of erythematous and brownish irregular‐shaped patches and tender cutaneous nodules 0.5–1.0 cm in diameter. Central necrosis and shallow ulcers were seen in the lesions. The patient also suffered from general fatigue, arthralgia, and weight loss. She was anemic and thrombocytopenic and had progressive impairment of liver function with coagulation defect. Histopathological study of skin lesions showed lobular panniculitis without vasculitis in the subcutaneous fat tissue. In the panniculitis lesion, moderate mixed cell infiltration consisting of lymphocytes and histiocytes was observed. Bone marrow aspiration revealed an increase in the number of histiocytes, mostly immature with active phagocytosis of erythroid cells, myeloid cells, and platelets. She was diagnosed as having malignant histiocytosis and treated with cyclophosphamide, vincristine, and prednisolone which she responded well; her fever subsided and the lesions healed with hyperpigmentation. In this patient, benign histiocytes with hemophagocytosis without immature forms were found in the skin lesions. According to our knowledge, this is the first Thai report of malignant histiocytosis with clinical features of panniculitis.
Psoriasis, Necrobiosis Lipoidica, Granuloma Annulare, Vitiligo and Skin Infections in the Same Diabetic Patient
Authors: Abraham Zeev , Lahat Nitza , Kinarty Amalia , Feuerman Eleasar J. 
Published: April 2015

AbstractA diabetic patient is described presenting psoriasis, necrobiosis lipoidica diabeticorum, granuloma annulare, and vitiligo and with a history of recurrent erysipelas and mycotic infections. Scrupulous physical examination excluded further systemic or cutaneous involvement. The immunological workup revealed both phenotypic and functional defects in cellular immunity.
Defective DNA Repair in Cultured Melanocytes from Xeroderma Pigmentosum Patients
Authors: Yamaguchi Jun , Mamada Akira , Kondo Seiji , Satoh Yoshiaki 
Published: April 2015

AbstractThe DNA repair of ultraviolet (UV)‐induced damages in primary cultured melanocytes from xeroderma pigmentosum (XP) patients and normal subjects were studied by measuring unscheduled DNA synthesis (UDS) on autoradiographs. Melanocytes were cultured in α‐minimum essential medium (α‐MEM) supplemented with 10% fetal calf serum (FCS), 12‐O‐tetradecanoyl‐phorbol‐13 acetate (TPA), and geneticin. The levels of UDS in XP melanocytes were compared with those in normal melanocytes.In both normal and XP melanocytes, post‐UV‐UDS increased dose‐dependently at doses of 5–10 J/m2. XP melanocytes exhibited various levels of defect in DNA repair, depending on the type of XP. Melanocytes from XP‐A patients displayed very low levels of UDS, only 6.2–8.4% that of the normal melanocytes. However, UDS values in melanocytes from intermediate groups, XP‐D, XP‐E, and XP‐F, were relatively high, 37.2–53.5% of the control in XP‐D, 50.0–66.5% in XP‐E, and 38.2–46.7% in XP‐F, respectively. Melanocytes from XP‐variant patients exhibited almost normal levels of UDS, 87.7–91.6% of those from normal subjects. The levels of UDS in XP melanocytes were very similar to those in fibroblasts isolated from the same specimens.
A Case of Sneddon's Syndrome with Positive ANA and Anti‐cardiolipin Antibodies: Primary Anti‐phospholipid Syndrome?
Authors: Otoyama Kazunobu , Katayama Ichiro , Suzuki Yuhsuke , Tone Takeshi , Nishioka Kiyoshi , Nishiyama Shigeo 
Published: April 2015

AbstractA 22‐year‐old woman developed ulcerative lesions on the lower extremities which usually exacerbated during the summer. Histological analysis revealed a micro‐thrombotic lesion in the deep dermis without inflammatory cell infiltration or fibrinoid degeneration of blood vessels. Magnetic resonance imaging revealed multiple cerebral infarctions. Abnormal laboratory findings included an elevated anti‐cardiolipin antibody titer and positive speckled pattern ANA (x80), but without other manifestations or signs of SLE. FACS analysis revealed that the patient's serum reacted with ethanol fixed endothelial cells in addition to keratinocytes and peripheral blood neutrophils. This case was thought to be livedo reticularis and cerebral thrombotic lesions (Sneddon's syndrome) associated with atrophie blanche or livedo(id) vasculitis and may be one clinical subset of primary anti‐phospholipid syndrome.
Malignant Eccrine Tumor
Authors: Nakamura Shin‐ichi , Imai Toshiya , Nakayama Keiji , Goto Masanori , Nishihara Kiyoshi , Hoshi Kenji 
Published: April 2015

AbstractA case of malignant eccrine tumor and its histological findings were reported. In this case, we considered basal cell carcinoma or malignant melanoma clinically; however, the histological features matched those of malignant eccrine tumor.It has been said that it is very difficult to determine the origin of tumors of sweat apparatus; to contribute to this ongoing dialogue, the classification of tumors of eccrine sweat apparatus was also discussed.