bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simulated fire injury: effects of trunk girdling and partial defoliation on reproductive development of apple trees (Malus domestica)
Authors: Rossouw Gerhard C. , Idowu Oluyoye , Gregson Aphrika , Holzapfel Bruno P. 
Published: August 2024

AbstractFire damage can significantly impact fruit productivity in orchards. However, the effects of nonlethal fire injuries on the reproductive development of apple trees remain poorly understood. To investigate these effects, we implemented three treatments: trunk girdling to simulate fire injury to xylem, defoliation of a third of the canopy (simulated crown fire injury), and a combined treatment (simulated surface fire injury), alongside a control. The experiment was conducted during the 2021–22 growing season using a randomised block design with four biological replicate plots. Girdling was less effective than crown and surface fire treatments in influencing fruit composition during the current growing season, and flowering and fruiting in the following season. The crown and surface fire treatments induced localised detrimental effects on fruit sugar and titratable acidity while stimulating peel blush. Additionally, these treatments led to reduced starch reserves by harvest, which likely disrupted subsequent flowering and crop load near the previously defoliated sections of the canopy. When surface fires damage leaves near the base of the canopy in addition to the trunk, fruit production in the lower part of the canopy is more likely to be compromised in the following season. Crown fires, which cause leaf loss near the apex of the canopy, appear to be particularly detrimental to tree productivity, as the top defoliation treatment impaired carbohydrate reserves in shoot terminals and roots. In conclusion, fire-induced loss of leaf area during fruit growth alters fruit composition in the current growing season and may lead to lower yields in the subsequent season.
Electrochemical detection of anti-tissue transglutaminase antibody using quantum dots-doped polypyrrole-modified electrode
Authors: Dumitriu Cristina , Pandele Andreea Madalina , Mîndroiu Mihaela Vasilica , Lazar Oana-Andreea , Popp Alina , Enachescu Marius , Buica George-Octavian 
Published: August 2024

AbstractA nanohybrid-modified glassy carbon electrode based on conducting polypyrrole doped with carbon quantum dots (QDs) was developed and used for the electrochemical detection of anti-tissue transglutaminase (anti-tTG) antibodies. To improve the polypyrrole conductivity, carrier mobility, and carrier concentration, four types of carbon nanoparticles were tested. Furthermore, a polypyrrole-modified electrode doped with QDs was functionalized with a PAMAM dendrimer and transglutaminase 2 protein by cross-linking with N-hydroxysuccinimide (NHS)/N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC). The steps of electrode surface modification were surveyed via electrochemical measurements (differential pulse voltammetry (DPV), impedance spectroscopy, and X-ray photoelectron spectroscopy (XPS)). The surface characteristics were observed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and contact angle measurements. The obtained modified electrode exhibited good stability and repeatability. DPV between − 0.1 and 0.6 V (vs. Ag/AgCl 3 M KCl reference electrode) was used to evaluate the electrochemical alterations that occur after the antibody interacts with the antigen (transglutaminase 2 protein), for which the limit of detection was 0.79 U/mL. Without the use of a secondary label, (anti-tTG) antibodies may be detected at low concentrations because of these modified electrode features.
Graphical Abstract
New global minimum conformers for the Pt$$_{19}$$ and Pt$$_{20}$$ clusters: low symmetric species featuring different active sites
Authors: Guevara-Vela José Manuel , Gallegos Miguel , Rocha-Rinza Tomás , Muñoz-Castro Álvaro , Kessler Peter L. Rodríguez , Martín Pendás Ángel 
Published: August 2024

Abstract

Context

The study of platinum (Pt) clusters and nanoparticles is essential due to their extensive range of potential technological applications, particularly in catalysis. The electronic properties that yield optimal catalytic performance at the nanoscale are significantly influenced by the size and structure of Pt clusters. This research aimed to identify the lowest-energy conformers for Pt$$_{18}$$

18

, Pt$$_{19}$$

19

, and Pt$$_{20}$$

20

species using Density Functional Theory (DFT). We discovered new low-symmetry conformers for Pt$$_{19}$$

19

and Pt$$_{20}$$

20

, which are 3.0 and 1.0 kcal/mol more stable, respectively, than previously reported structures. Our study highlights the importance of using density functional approximations that incorporate moderate levels of exact Hartree-Fock exchange, alongside basis sets of at least quadruple-zeta quality. The resulting structures are asymmetric with varying active sites, as evidenced by sigma hole analysis on the electrostatic potential surface. This suggests a potential correlation between electronic structure and catalytic properties, warranting further investigation.

Methods

An equivariant graph neural network interatomic potential (NequIP) within the Atomic Simulation Environment suite (ASE) was used to provide initial geometries of the aggregates under study. DFT calculations were performed with the ORCA 5 package, using functional approximations that included Generalized Gradient Approximation (PBE), meta-GGA (TPSS, M06-L), hybrid (PBE0, PBEh), meta-GGA hybrid (TPSSh), and range-separated hybrid ($$\omega $$
ω
B97x) functionals. Def2-TZVP and Def2-QZVP as well as members of the cc-pwCVXZ-PP family to check basis set convergence were used. QTAIM calculations were performed using the AIMAll suite. Structures were visualized with the AVOGADRO code.
Seepage monitoring and leaks detection along an earth dam with a multi-sensor thermal-active system
Authors: Radzicki Krzysztof , Stoliński Marek 
Published: August 2024

AbstractThermal investigation using linear sensors installed in the ground is currently one of the most effective methods for analyzing seepage processes in the bodies of earth dams or dikes and their subsoil, including leaks detection. However, linear, fiber-optic temperature sensors present several limitations when applied to existing structures, including installation difficulties and substantial costs. To enhance the applicability of the thermal method for studying subsurface water flow, a novel Multi-Point Thermal-Active Monitoring method has been devised. The approach consists of installing, through hammering, thermal-active sensors side-by-side in the ground, at the same depth in a single line thereby achieving quasi-continuous measurements along the structure. Small openings from sensor installation are secured with bentonite and do not pose a threat to the structure. Each sensor is equipped with a micro-heater, which enables the use of the thermo-active method to determine in-situ seepage velocity. This article describes this method and its initial application in 2014 on one of the Polish earth dams. This application enabled the detection of two leakage zones and the monitoring of their flow velocities, leading to the successful validation of the described technology.
Prospective analysis of STRATAFIX™ symmetric PDS plus suture for fascial closure in spinal surgery: a pilot study
Authors: Glener Steven R. , Patel Pious D. , Serva Stephanie N. , Self Dwight Mitchell , Heller Joshua E. 
Published: August 2024

AbstractWound closure is an integral part of every spinal procedure. Effective and secure wound closure is paramount in the prevention of infection, wound dehiscence and the preservation of cosmesis. Barbed suture technologies such as STRATAFIX™ Symmetric have been studied and are used in a variety of specialties, including obstetrics and orthopedic surgery, but is underutilized in neurosurgery. This study aims to assess the time and rate of closure using STRATAFIX™ Symmetric technology for fascial closure and compare this method to the more traditionally used method of fascial closure using braided absorbable sutures below the epidermis. 20 patients were recruited for the study. 10 patients underwent fascial approximation with braided absorbable sutures and definitive fascial closure with STRATAFIX™ Symmetric. In the control group, fascial closure was completed entirely with interrupted braided absorbable stitches. Patients assigned to STRATAFIX™ Symmetric group had shorter mean time for fascial closure, faster rate of average fascial closure, and lower number of total sutures used. The use of barbed suture technology such as STRATAFIX™ Symmetric may reduce the time to closure in thoracolumbar spine surgery without increasing the risk of adverse events. This pilot study forms the framework for a larger randomized, controlled trial appropriately powered for such an analysis.
Impact of tumor multiplicity on the prognosis of patients with primary renal cell carcinoma: a SEER database analysis
Authors: Yang Tianyue , Zheng Hongfeng , Chen Shaojun , Gong Min , Liu Yifan , Zhou Wang , Ye Jianqing , Pan Xiuwu , Cui Xingang 
Published: August 2024

AbstractTo compare clinical characteristics and survival outcomes of patients with multiple renal cell carcinoma versus single renal cell carcinoma. Develop a prognostic model for predicting prognosis in patients with multiple tumors and analyze prognostic factors. Patients with primary multiple renal cell carcinoma were selected from the Surveillance, Epidemiology, and End Results database (2004–2015). They were divided into single-tumor and multiple-tumor groups. Survival analysis was conducted using the Kaplan–Meier method and log-rank test. A Cox regression model was used to identify potential prognostic factors. A total of 19,489 renal cell carcinoma cases were included, with 947 in the multiple-tumor group and 18,542 in the single-tumor group. The multiple-tumor group had lower cancer-specific survival (P = 0.03, HR = 1.431). Cox regression identified risk factors for the multiple-tumor group including number of tumors, gender, combined summary stage, T stage, N stage, tumor size, and type of surgery. The predicted probabilities showed acceptable agreement with the actual observations at 3-, 5-, and 8-years area under the curve values in both the training and validation cohorts (0.831 vs. 0.605; 0.775 vs. 0.672; and 0.797 vs. 0.699, respectively). Compared with single renal cell carcinoma, multiple renal cell carcinoma is associated with decreased cancer-specific survival. Additionally, we identified several prognostic factors including the number of tumors, T stage, tumor size, and type of surgery. These findings offer valuable insights for selecting appropriate treatment strategies for patients diagnosed with multiple renal cell carcinomas.
Barriers to Progress Feedback Adoption in Outpatient Geriatric Mental Healthcare: Exploring Age-Related Factors - A Qualitative Study
Authors: Frissen L. N. , Janse P. D. , Roskam R. V. , Hendriks G. J. 
Published: August 2024

AbstractMonitoring treatment progress through progress feedback is recognized for its efficacy and demonstrated value. However, its integration and utilization within treatments still need to be improved. Insufficient understanding exists regarding the factors within geriatric mental healthcare influencing the adoption of progress feedback. This study aimed to explore the determinants impacting the utilization of progress feedback within outpatient geriatric mental healthcare, specifically focusing on age-related perspectives and patient group characteristics. This qualitative investigation employed semi-structured interviews involving clinicians (N = 14) selected from four outpatient geriatric teams. The findings revealed both inhibiting and facilitating perspectives concerning progress feedback. Clinicians preferred user-friendly, specific, and tailored measures. Challenges included organizational support, integration in work processes, training, and the digital progress feedback system. Age-related perspectives such as older adults’ diverse issues, limited digital skills, and cognitive problems hindered implementation, particularly in the oldest generation of older patients. In outpatient geriatric mental healthcare, many factors and attitudes influencing progress feedback align with those observed in adult psychiatry literature. Moreover, this study highlights specific age-related factors that impede the adoption and implementation of progress feedback, shedding light on the specific barriers within this context.
Transforming the Effectiveness and Equity of a Psychological Therapy Service: A Case Study in the English NHS Talking Therapies Program
Authors: James Katy , Saxon David , Barkham Michael 
Published: August 2024

AbstractTo work with a psychological therapies service to implement a recovery plan, as required by a government body, aimed at improving patient outcomes (effectiveness) and decreasing practitioner variability (equity). A case-study utilizing components of a learning health system, including nationally mandated patient outcome data, comprising three 18-month phases: (1) retrospective baseline; (2) improving patient outcomes (management-led); and (3) reducing practitioner variability (clinician-led). Primary analyses focused on 35 practitioners (NPR = 35) who were constant across the three phases and their patients in each phase (NPA = 930, 1226, 1217, respectively). Reliable improvement rates determined patient outcomes and multilevel modeling yielded practitioner effects. To test generalizability, results were compared to the whole practitioner sample for each phase: (1) NPR = 81, NPA = 1982; (2) NPR = 80, NPA = 2227; (3) NPR = 74, NPA = 2267. Ethical approval was granted by the Health Research Authority. Patient outcomes improved in successive phases for both the core and whole practitioner samples with the largest impact occurring in the management-led intervention. Practitioner variability decreased in successive phases in both the core and whole practitioner samples except in the management-led intervention of the whole sample. Compared with the management-led intervention, the practitioner-led intervention yielded a decrease in practitioner effect exceeding 60% in the core sample and approaching 50% in the whole sample. The implementation of multiple components of a learning health system can lead to improvements in both the effectiveness and equity of a psychological therapy service.
Prognostic and predictive impact of NOTCH1 in early breast cancer
Authors: Engel Julia , Wieder Vanessa , Bauer Marcus , Kaufhold Sandy , Stückrath Kathrin , Wilke Jochen , Hanf Volker , Uleer Christoph , Lantzsch Tilmann , Peschel Susanne , John Jutta , Pöhler Marleen , Weigert Edith , Bürrig Karl-Friedrich , Buchmann Jörg , Santos Pablo , Kantelhardt Eva Johanna , Thomssen Christoph , Vetter Martina 
Published: August 2024

Abstract

Purpose
Systemic therapy plays a major part in the cure of patients with early breast cancer (eBC). However, personalized treatment concepts are required to avoid potentially harmful overtreatment. Biomarkers are pivotal for individualized therapy. The Notch signalling pathway is widely considered as a suitable prognostic or predictive marker in eBC. This study aimed primarily at assessing the relationship between NOTCH1 mRNA expression levels and histopathological features of breast cancer tumors, as well as clinical characteristics of the correspondent eBC patients. As a secondary aim, we investigated the prognostic and predictive value of NOTCH1 by assessing possible associations between NOTCH1 mRNA expression and recurrence-free interval (RFI) and overall survival after five years of observation.

Patients and methods
The relative NOTCH1 mRNA expression was determined in 414 tumour samples, using quantitative PCR in a prospective, multicenter cohort (Prognostic Assessment in Routine Application (PiA), 2009–2011, NCT01592825) of 1,270 female eBC patients.

Results
High NOTCH1 mRNA expression was detected in one-third of the tumours and was associated with negative hormone receptor status and high uPA/PAI-1 status. In addition, high NOTCH1 mRNA expression was found to be associated with more RFI related events (adjusted hazard ratio 2.1, 95% CI 1.077–4.118). Patients who received adjuvant chemotherapy and had high NOTCH1 mRNA expression in the tumour (n = 86) were three times more likely to have an RFI event (adjusted hazard ratio 3.1, 95% CI 1.321–7.245, p = 0.009).

Conclusion
In this cohort, NOTCH1 mRNA expression had a prognostic and predictive impact. Tumours with high NOTCH1 mRNA expression may be less sensitive to cytotoxic treatment and downregulation of the Notch signalling pathway (e.g. by γ-secretase inhibitors) may be valuable for eBC therapy as an individualised treatment option.
Insight into the Interaction of Perovskite-Like Surfaces (LaMnO3 and LaCoO3) with Ar, H2, CO, and O2 through NAP-XPS Analysis
Authors: Tapia-P. Juan , Gallego Jaime , Gamba Oscar , Espinal Juan F. 
Published: August 2024

AbstractPerovskite-like oxides present huge chemical variability and a wide range of applications as catalysts for oxidation reactions. The interaction of several small gas molecules with the surface of LaCoO3 and LaMnO3 perovskite-like oxides was studied by Near Ambient Pressure X-ray photoelectron spectroscopy (NAP-XPS) and CO Temperature Programmed Desorption (CO-TPD). Surface chemical changes such as the Osurf/Olattice and cation B oxidation state ratios were analyzed as a function of temperature (400 K, 450 K, 500 K, 550 K, and 650 K) under different gas atmospheres like Ar, CO, H2, and O2. It was found that there was a partial surface reduction when H2 and CO were used in the reaction, and therefore, the cation B oxidation state (Mn4+/Mn3+ and Co3+/Co2+) ratio decreased. Under the CO stream, carbonate species were formed, presenting a C1s signal between 284.5 eV and 287 eV. The CO2 evolution during the reaction at temperatures greater than 500 K was associated with CO activation over or near to surface oxygen species. A Mars-van Krevelen mechanism was proposed for the process, finding significant differences between LaCoO3 and LaMnO3 perovskite-like solid catalysts behavior.
Graphical Abstract
Preschool Engineering Play on Nature Playscapes
Authors: Yuan Yuchang , Zeng Wen , Kloos Heidi , Brown Rhonda , Carr Victoria 
Published: August 2024

AbstractAs an increasingly recognized facet of early childhood development, the integration of play into early STEM education is garnering attention. This paper delves into the role of engineering play within early childhood education, emphasizing its application in natural playscape settings. The focus is on investigating the extent to which engineering play can spontaneously emerge in such natural settings. To explore this, we analyzed extensive video footage of preschool children engaging in play on these playscapes. Our findings reveal a spontaneous occurrence of engineering play, highlighting three illustrative cases. These cases provide valuable insights into how playscapes, complemented by strategic adult involvement, can nurture young children’s engineering skills and behaviors. The findings contribute to the growing evidence that young children are capable explorers, particularly in environments that offer a harmonious mix of structured and unstructured elements tailored to their developmental needs. This research has significant implications for early childhood education. It underscores the importance of incorporating engineering concepts into play-based learning and advocates for a nature-oriented pedagogical approach and curriculum. This approach not only promotes engineering thinking and practices among young learners but also advocates for a curriculum that nurtures these skills through playful, natural interactions.
Navigating Spatial Ability for Mathematics Education: a Review and Roadmap
Authors: Schenck Kelsey E. , Nathan Mitchell J. 
Published: August 2024

AbstractSpatial skills can predict mathematics performance, with many researchers investigating how and why these skills are related. However, a literature review on spatial ability revealed a multiplicity of spatial taxonomies and analytical frameworks that lack convergence, presenting a confusing terrain for researchers to navigate. We expose two central challenges: (1) many of the ways spatial ability is defined and subdivided are often not based in well-evidenced theoretical and analytical frameworks, and (2) the sheer variety of spatial assessments. These challenges impede progress in designing spatial skills interventions for improving mathematics thinking based on causal principles, selecting appropriate metrics for documenting change, and analyzing and interpreting student outcome data. We offer solutions by providing a practical guide for navigating and selecting among the various major spatial taxonomies and instruments used in mathematics education research. We also identify current limitations of spatial ability research and suggest future research directions.
The best ends by the best means: ethical concerns in app reviews
Authors: Tjikhoeri Neelam , Olson Lauren , Guzmán Emitzá 
Published: August 2024

AbstractThis work analyzes ethical concerns found in users’ app store reviews. We performed this study because ethical concerns in mobile applications (apps) are widespread, pose severe threats to end users and society, and lack systematic analysis and methods for detection and classification. In addition, app store reviews allow practitioners to collect users’ perspectives, crucial for identifying software flaws, from a geographically distributed and large-scale audience. For our analysis, we collected five million user reviews, developed a set of ethical concerns representative of user preferences, and manually labeled a sample of these reviews. We found that (1) users highly report ethical concerns about censorship, identity theft, and safety (2) user reviews with ethical concerns are longer, more popular, and lowly rated, and (3) there is high automation potential for the classification and filtering of these reviews. Our results highlight the relevance of using app store reviews for the systematic consideration of ethical concerns during software evolution.