bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRANSCRIPTION ACTIVATOR-LIKE EFFECTOR NUCLEASE-Mediated Generation and Metabolic Analysis of Camalexin-Deficient cyp71a12 cyp71a13 Double Knockout Lines
Authors: Müller Teresa M. , Böttcher Christoph , Morbitzer Robert , Götz Cornelia C. , Lehmann Johannes , Lahaye Thomas , Glawischnig Erich 
Published: June 2015

Abstract
In Arabidopsis (Arabidopsis thaliana), a number of defense-related metabolites are synthesized via indole-3-acetonitrile (IAN), including camalexin and indole-3-carboxylic acid (ICOOH) derivatives. Cytochrome P450 71A13 (CYP71A13) is a key enzyme for camalexin biosynthesis and catalyzes the conversion of indole-3-acetaldoxime (IAOx) to IAN. The CYP71A13 gene is located in tandem with its close homolog CYP71A12, also encoding an IAOx dehydratase. However, for CYP71A12, indole-3-carbaldehyde and cyanide were identified as major reaction products. To clarify CYP71A12 function in vivo and to better understand IAN metabolism, we generated two cyp71a12 cyp71a13 double knockout mutant lines. CYP71A12-specific transcription activator-like effector nucleases were introduced into the cyp71a13 background, and very efficient somatic mutagenesis was achieved. We observed stable transmission of the cyp71a12 mutation to the following generations, which is a major challenge for targeted mutagenesis in Arabidopsis. In contrast to cyp71a13 plants, in which camalexin accumulation is partially reduced, double mutants synthesized only traces of camalexin, demonstrating that CYP71A12 contributes to camalexin biosynthesis in leaf tissue. A major role of CYP71A12 was identified for the inducible biosynthesis of ICOOH. Specifically, the ICOOH methyl ester was reduced to 12% of the wild-type level in AgNO3-challenged cyp71a12 leaves. In contrast, indole-3-carbaldehyde derivatives apparently are synthesized via alternative pathways, such as the degradation of indole glucosinolates. Based on these results, we present a model for this surprisingly complex metabolic network with multiple IAN sources and channeling of IAOx-derived IAN into camalexin biosynthesis. In conclusion, transcription activator-like effector nuclease-mediated mutation is a powerful tool for functional analysis of tandem genes in secondary metabolism.
The structure of a GFP-based antibody (fluorobody) to TLH, a toxin fromVibrio parahaemolyticus
Authors: Chen Yaoguang , Huang Xiaocheng , Wang Rongzhi , Wang Shihua , Shi Ning 
Published: June 2015

A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteriumVibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space groupP43212, with unit-cell parametersa=b= 63.35,c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to anRfreeof 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.
The association between learning styles and perception of teaching quality
Authors: Jepsen Denise M. , Varhegyi Melinda M. , Teo Stephen T.T. 
Published: June 2015

Purpose
– Although learning styles and teaching quality have been studied separately, the association between the association between the two has yet to be identified. The purpose of this paper is to establish the relationship between students’ learning styles with students’ perceptions of teaching quality.

Design/methodology/approach
– The study used survey responses from 272 undergraduate students. All 80 items in the Honey and Mumford’s (1986) Learning Styles Questionnaire and all 46 teaching quality items (Thompson, 2002) were used to assess learning styles and perceptions of teaching quality, respectively. Structural equation modelling was used to investigate the relationships between learning styles and perception of teaching quality.

Findings
– Results indicate learners with dominant reflector or activist styles are influenced in their perceptions of teaching quality of their teacher or lecturer. No perceptions of teaching quality relationships were found for students with dominant theorist or pragmatist learning style.

Practical implications
– Recognising that perceptions of teaching quality impacts on some students, teachers and lecturers may consider and articulate the type of learning they would prefer students to adopt for a particular class. As an example, a teacher might ask students who would normally see themselves as active learners to relax into the lecture mode of delivery and reflect on what is said in the lecture, to take time to consider what is said.

Originality/value
– This study combines the two important constructs of learning and perception of teaching quality to provide insight into the relationship between the two.
Employability skills: perspectives from a knowledge-intensive industry
Authors: Collet Chris , Hine Damian , du Plessis Karen 
Published: June 2015

Purpose
– While the global education debate remains focused on graduate skills and employability, the absence of a shared language between student, academic and industry stakeholder groups means that defining industry skills requirements is both essential and difficult. The purpose of this paper is to assess graduate skills requirements in a knowledge-intensive industry from a demand perspective as distinct from a curriculum (supply) viewpoint.

Design/methodology/approach
– Skills items were derived from a breadth of disciplines across academic, policy and industry literature. CEOs and senior managers in the innovation and commercialisation industry were surveyed regarding perceptions of skills in graduates and skills in demand by the firm. Two rounds of exploratory factor analyses were undertaken to examine employers’ perceptions of the skills gap.

Findings
– First-order analysis resolved ten broad constructs that represent cognitive, interpersonal and intrapersonal skills domains as applied in this industry. Knowledge, leadership and interprofessional collaboration feature as prominent skills. Second-order analysis revealed employers’ perceptions of graduate skills specifically centre on organisational fit and organisational success. An over-arching theme relates to performance of the individual in organisations.

Research limitations/implications
– The findings suggest that the discourse on employability and the design of curriculum need to shift from instilling lists of skills towards enabling graduates to perform in a diversity of workplace contexts and expectations centred on organisational purpose.

Originality/value
– In contrast to the heterogeneous nature of industry surveys, the authors targeted a homogenous sector that is representative of knowledge-intensive industries. This study contributes to the broader stakeholder dialogue of the value and application of graduate skills in this and other industry sectors.
Anti-social behaviour and European protection against eviction
Authors: Vols M. , Tassenaar P.G. , Jacobs J.P.A.M. 
Published: June 2015

Purpose
– The purpose of this paper is to assess the implementation of the minimum level of protection against the loss of the home that arises from Article 8 of the European Convention on Human Rights in The Netherlands. The paper focuses on anti-social behaviour-related cases in which the landlord requests the court to issue an eviction order.

Design/methodology/approach
– The paper is based on a statistical analysis of nearly 250 judgements concerning housing-related anti-social behaviour.

Findings
– A significant difference is found in the court’s attitude against drug-related anti-social behaviour and other types of nuisance. Moreover, it is found that in two-thirds of the cases, the tenant advanced a proportionality defence. Although the European Court stresses the need of a proportionality check, the Dutch courts ignore the tenant’s proportionality defence in 10 per cent of the cases and issue an eviction order in the majority of all cases. Advancing a proportionality defence does not result in any difference for the court decision.

Originality/value
– The paper presents original data on the legal protection against eviction in cases concerning anti-social behaviour. This is the first study that analyses the approach towards housing-related anti-social behaviour in the context of the European minimum level of protection. Whilst centred on legislation and procedures in The Netherlands, its findings and discussion are relevant in other jurisdictions facing similar issues.
What will the “Eyes and Ears” of the SEC choose to see and hear this year? OCIE announces examination priorities for 2015
Authors: Berman Kenneth , Harrell Michael P. , Larkin Gregory 
Published: June 2015

Purpose
– To discuss and interpret the recently published summary of the select priorities of the SEC’s Office of Compliance Inspections and Examinations (“OCIE”) in connection with the National Exam Program for 2015.

Design/methodology/approach
– This article highlights the broad OCIE focus areas and provides detail on the associated initiatives.

Findings
– OCIE’s priorities appear to place particular emphasis on retail investors and investors saving for retirement and market-wide risks, including cybersecurity. The examination priorities also emphasize OCIE’s evolving ability to analyze data to identify and examine registrants that may be engaged in illegal activity. Of particular interest to private equity fund sponsors, the National Exam Program will continue to conduct examinations that focus on fees and expenses borne by investors in private equity funds.

Practical implications
– In view of the OCIE’s priorities, recent public comments by OCIE officials (concerning, for example, presentation of performance data) and our experience representing private equity firms being examined by OCIE, private equity fund sponsors should continue to be prepared for rigorous examinations on these issues and the areas of focus highlighted by the SEC in the past three years.

Originality/value
– The article summarizes the OCIE’s recently published examination priorities for 2015 that cover a broad range of market participants and target a variety of their products, practices and procedures, including a continued focus on private equity fund sponsors.
SEC asset management unit’s enforcement priorities for 2015 – conflicts of interest
Authors: Weiner Perrie Michael , Hunnius Patrick , Crain Sean R. 
Published: June 2015

Purpose
– To address “Conflicts, Conflicts Everywhere,” a speech at the recent IA Watch 17th Annual Compliance Conference by Julie M. Riewe, co-chief of the Securities and Exchange Commission’s Enforcement Division’s Asset Management Unit (AMU).

Design/methodology/approach
– Provide information on the AMU’s creation, the AMU’s 2015 priorities for each of the primary investment vehicles it polices –registered investment companies; private funds (both hedge funds and private equity funds); and other client accounts, such as separately managed accounts/retail accounts – and the AMU’s central concern across all of the investment vehicles it polices: conflicts of interest.

Findings
– Conflicts of interest will be receiving much attention from the Commission in the coming months. In order to help avoid an SEC inquiry or, worse yet, an enforcement action, corporations and individuals should seek counsel.

Originality/value
– Practical explanation and guidance from experienced securities and financial services lawyers.
The effect of customer incivility on service employees’ customer orientation through double-mediation of surface acting and emotional exhaustion
Authors: Hur Won-Moo , Moon Tae Won , Han Su-Jin 
Published: June 2015

Purpose– The purpose of this paper is to examine how customer incivility affects service employees’ emotional labor (i.e. surface acting) and the way surface acting augments their emotional exhaustion at work, and in turn, damages customer orientations of service employees.Design/methodology/approach– Using a sample of 309 department store sales employees in South Korea, a two-stage mediation model is used in terms of structural equation modeling.Findings– The results indicate that customer incivility is positively related to service employees’ use of surface acting; this, in turn, results in feelings of emotional exhaustion, which are negatively related to their customer orientation. That is, the findings of this study shows that the negative relationship between customer incivility and service employees’ customer orientation was fully and sequentially mediated by service employees’ surface acting and emotional exhaustion.Research limitations/implications– The main limitation is the nature of the cross-sectional data the authors used in the analysis. It gives us reason to be very cautious in reaching conclusions concerning causal relationships among variables, since the authors did not capture longitudinal variation.Practical implications– The research shows that customer incivility has a negative effect on service employees’ customer-oriented behaviors since experiences of customer incivility among emotionally exhausted employees via surface acting generates inadequate and unfair sense-making related to the treatment offered by customers, which increases the tendency of decreasing their effort and loyalty for customers to prevent further loss of emotional resources. Therefore, service organizations should devise appropriate strategies and implement systematic programs for reducing employee exposure to customer incivility, or preventing it altogether.Originality/value– The current study broadens the conceptual work and empirical studies in customer incivility literature by representing a fundamental mechanism of why customer incivility negatively affects service employees’ customer orientation. The primary contribution of the study is to gain a deeper understanding of how customer incivility leads to lower employee customer-oriented behaviors through double mediating effects of surface acting and emotional exhaustion.
Predicting determinants of Internet banking adoption
Authors: Sharma Sujeet Kumar , Govindaluri Srikrishna Madhumohan , Al Balushi Shahid M. 
Published: June 2015

Purpose
– The purpose of this paper is to explore the main determinants of Internet banking users on the basis of literature of technology acceptance model (TAM). Understanding and predicting main determinants of Internet banking is an important issue for banking industry and users.

Design/methodology/approach
– Service quality and trust were incorporated in the TAM together with demographic variables. The data were collected using Google Docs from 110 Omani Internet banking users. A two-staged regression-neural network model was applied to understand and predict Internet banking adoption.

Findings
– The results obtained from multiple linear regression model were compared with the results from neural network model to predict Internet banking adoption and the performance of latter model was found to superior. The neural network model was able to capture relative importance of all independent variables, service quality, trust, perceived usefulness, perceived ease of use, attitude and demographic variables, whereas perceived ease of use and demographic variables were not significant predictors of Internet banking adoption as per the regression model.

Practical implications
– This study provides useful insights with regard to development of Internet banking systems to banking professionals and information systems researchers in Oman and similar emerging economies.

Originality/value
– This study is probably the first attempt to model Internet banking adoption in Gulf Cooperation Council using a predictive rather than explanatory focus. The majority of studies in Internet banking adoption in Oman and elsewhere usually utilize modeling methods suited for explanatory purposes.
The furoxan nitric oxide donor, PRG150, evokes dose‐dependent analgesia in a rat model of painful diabetic neuropathy
Authors: Huang Lillian Y , Tsui Debbie Y , Williams Craig M , Wyse Bruce D , Smith Maree T 
Published: June 2015

SummaryPainful diabetic neuropathy (PDN) is a type of peripheral neuropathic pain that is often intractable. Elevated nitric oxide (NO) from neuronal and non‐neuronal sources in the somatosensory system is implicated in the pathobiology of peripheral neuropathic pain. However, in diabetes, nitrergic nerve degeneration to deplete NO bioactivity appears causal in the pathogenesis of irreversible autonomic neuropathy, another long term complication of diabetes. Hence, this study hypothesized that progressive NO depletion may underpin the pathobiology of PDN and that NO donors may alleviate PDN. Diabetes was induced in rats with intravenous streptozotocin (STZ) at 70 mg/kg and confirmed if blood glucose levels (BGLs) on day 10 post‐STZ were ≥15 mmol/L. Analgesic efficacy of subcutaneous (s.c.) bolus doses of the furoxan NO donor, PRG150 was assessed in the STZ‐diabetic rat model of PDN at 10‐, 14‐ and 24‐weeks post‐STZ relative to the sydnominine NO donor, SIN‐1 and its prodrug, molsidomine. PRG150 produced dose‐dependent analgesia in STZ‐diabetic rats whereas SIN‐1 and molsidomine evoked neuro‐excitatory side‐effects, but not analgesia. The 1000‐fold larger doses of PRG150 needed to produce analgesia at 14‐ and 24‐weeks (800 pmol/kg) c.f. 10‐weeks (8 fmol/kg) post‐STZ in rats, suggest that progressive NO depletion is also causal in PDN. Importantly, doses of PRG150 up to 10 000 fold higher than the analgesic dose did not produce hypotension in rats. The 50‐fold greater release of NO by SIN‐1 c.f. PRG150 in vitro, may underpin the neuro‐excitatory rather than analgesic effects of SIN‐1/molsidomine. PRG150 is worthy of further investigation as a potential novel analgesic for PDN.
PCC Elections as a ‘Failed Experiment’: What Lessons can be Learned?
Authors: Kirkland Christopher 
Published: June 2015

AbstractThe low turnout of the 2012 police and crime commissioner (PCC) elections have led to questions surrounding their legitimacy and have even led to the former Deputy Prime Minister Nick Clegg describing the elections—elections his party helped to introduce—as a ‘failed experiment’. Despite this, the election of a majority Conservative government in May 2015 appears to offer some longevity to the role of police and crime commissioners and cements next year's PCC elections in the political diary. Concerns in the immediate aftermath of the elections focused upon the costs of the elections. In this article I offer some suggestions as to what lessons could be learned from this experiment and, through exploring the attitudes of voters, political parties and the media, suggest that we can learn four lessons: (1) that spoilt ballots cannot be ignored; that (2) political parties and (3) the media's attitudes toward elections are important in encouraging people to vote; and (4) that high numbers of independent candidates cannot simply be welcomed at times of elections.
Physico‐Chemical and Structural Characteristics of Vegetables Cooked Under Sous‐Vide, Cook‐Vide, and Conventional Boiling
Authors: Iborra‐Bernad C. , García‐Segovia P. , Martínez‐Monzó J. 
Published: June 2015

AbstractIn this paper, physico‐chemical and structural properties of cut and cooked purple‐flesh potato, green bean pods, and carrots have been studied. Three different cooking methods have been applied: traditional cooking (boiling water at 100 °C), cook‐vide (at 80 and 90 °C) and sous‐vide (at 80 °C and 90 °C). Similar firmness was obtained in potato applying the same cooking time using traditional cooking (100 °C), and cook‐vide and sous‐vide at 90 °C, while in green beans and carrots the application of the sous‐vide (90 °C) required longer cooking times than cook‐vide (90 °C) and traditional cooking (100 °C). Losses in anthocyanins (for purple‐flesh potatoes) and ascorbic acid (for green beans) were higher applying traditional cooking. β‐Carotene extraction increased in carrots with traditional cooking and cook‐vide (P < 0.05). Cryo‐SEM micrographs suggested higher swelling pressure of starch in potatoes cells cooked in contact with water, such as traditional cooking and cook‐vide. Traditional cooking was the most aggressive treatment in green beans because the secondary walls were reduced compared with sous‐vide and cook‐vide. Sous‐vide preserved organelles in the carrot cells, which could explain the lower extraction of β‐carotene compared with cook‐vide and traditional cooking. Sous‐vide cooking of purple‐flesh potato is recommended to maintain its high anthocyanin content. Traditional boiling could be recommended for carrots because increase β‐carotenes availability. For green beans, cook‐vide, and sous‐vide provided products with higher ascorbic acid content.
The Fate of Organophosphorus Pesticides during Camellia Oil Production
Authors: Liu Yihua , Mo Runhong , Zhong Donglian , Shen Danyu , Ni Zhanglin , Tang Fubin 
Published: June 2015

AbstractThe purpose of this study was to investigate the fate of organophosphorus pesticides (OPs) during camellia oil production process, from camellia fruit to the final oil product. The results showed that the OPs were mainly distributed in the peel of camellia fruit, basically above 40% after the pesticide application of 7 d (P < 0.05). A small amount of OPs could enter into the seed and convert to crude camellia oil, with the concentration of 19.5 to 548.2 mg/L. In addition, metabolites of OPs (25.7 to 768.9 mg/L) could be detected in the crude camellia oil. M8oreover, the refining process (degumming, deacidfying, bleaching) had a significant effect on the removal of OPs from the crude camellia oil (P < 0.05), and the effect was related to the octanol–water partition constant (LogP) of pesticide. The larger the LogP, the more stable the OPs were during refining process. The final refined camellia oil was found to have no detectable levels of OPs metabolite.