bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cryptic diversity and biogeography of the widespread brown alga Colpomenia sinuosa (Ectocarpales, Phaeophyceae)
Authors: Lee Kyung Min , Boo Sung Min , Kain (Jones) Joanna M. , Sherwood Alison R. 
Published: December 2012

Abstract
Recent DNA sequencing studies have demonstrated that many widespread species consist of species complexes with high genetic diversity. The brown alga Colpomenia sinuosa is a suitable candidate for studies into cryptic diversity because of its cosmopolitan distribution in the world’s oceans. To examine the genetic diversity within the species and the relationships of the genetic lineages to current distribution, we analyzed a total of 134 cox3 sequences from C. sinuosa specimens collected in 19 countries; we also examined 16 rbcL from a subset of the specimens for comparative purposes. Phylogenetic trees and haplotype analyses of cox3 and rbcL revealed high cryptic diversity comprising three genealogical groups within C. sinuosa. Two groups overlapped in the Indo-Pacific and Atlantic regions, one was confined to the tropics, while the remaining group was localized in the Red Sea and western Mediterranean Sea. Thus, the current distribution of C. sinuosa may well result from a mixture of modern anthropogenic introductions and ancient relicts.
Moderate elevations of high-sensitivity cardiac troponin I and B-type natriuretic peptide in chronic hemodialysis patients are associated with mortality
Authors: Geerse Daniël A. , van Berkel Miranda , Vogels Steffie , Kooman Jeroen P. , Konings Constantijn J.A.M. , Scharnhorst Volkher 
Published: December 2012

Abstract

Background: Several biomarkers are associated with mortality in hemodialysis patients. In particular, elevated cardiac troponin T and B-type natriuretic peptide (BNP) are strong predictors of mortality; however, less is known about cardiac troponin I (cTnI). Elevated troponin I is detected in many hemodialysis patients, but the association of moderate elevations with mortality is unclear.

Methods: The relation between mortality and cTnI, using a high-sensitivity cTnI assay, as well as BNP and C-reactive protein (CRP) was evaluated in 206 chronic hemodialysis patients.

Results: Median follow-up was 28 months with a total mortality of 35%. Mortality was significantly associated with elevated cTnI, BNP and CRP. Even patients with only moderate elevation of cTnI (0.01–0.10 μg/L) showed 2.5-fold increased mortality. Interestingly, hazard ratios for mortality for single (random) measurements were comparable to those for mean/median measurements. Subsequently, subgroup analysis based on combined markers was performed. Patients with both cTnI <0.01 μg/L and BNP in the first quartile had 100% survival. Patients with either cTnI <0.01 μg/L or BNP in the lowest quartile had significantly lower mortality (12% and 13%, respectively) than patients with BNP levels in the second quartile or higher and cTnI of 0.01–0.05 μg/L and patients with cTnI ≥0.05 μg/L (mortality 46 and 58%, respectively).

Conclusions: A combination of moderate elevation of cTnI and BNP provided additional prognostic value. A single measurement of these biomarkers performed comparably to the mean/median of multiple measurements.
ADAM12s and PP13 as first trimester screening markers for adverse pregnancy outcome
Authors: Deurloo Koen L. , Linskens Ingeborg H. , Heymans Martijn W. , Heijboer Annemieke C. , Blankenstein Marinus A. , van Vugt John M.G. 
Published: December 2012

Abstract

Background: The aim of the study was to assess the screening performance of first trimester maternal serum measurements of A-disintegrin-and-metalloprotease 12-s (ADAM12s) and placental protein 13 (PP13) for preeclampsia (PE), gestational hypertension (GH) and small-for-gestational-age (SGA) fetuses.

Methods: In this retrospective case-control study 220 pregnant women were matched for gestational and maternal age at sampling. Results were expressed as multiples of the median (MoM) and compared using Kruskal-Wallis and Mann-Whitney U-test. Screening performance was assessed by receiver operator characteristics (ROC) curves and area under the curve (AUC).

Results: Seventeen cases of PE, 30 cases of GH and eight cases of SGA fetuses were matched with 165 controls. ROC-analysis yielded AUCs for ADAM12s and PP13 of 0.63 and 0.59 for PE, 0.68 and 0.57 for GH and 0.59 and 0.62 for SGA, respectively. Combined ADAM12 and PP13 did not improve the AUC value. When the specificity was set at 80%, corresponding detection rate of ADAM12s was 52% for GH.

Conclusions: Combined ADAM12s and PP13 measurements do not predict adverse pregnancy outcome, but decreased first trimester ADAM12s levels are associated with GH.
Biological variation and prognosis usefulness of new biomarkers in liver transplantation
Authors: Miguel Diego , Prieto Belén , Álvarez Francisco V. 
Published: December 2012

Abstract

Background: An observational retrospective study has been conducted, including 52 patients (37 male and 15 female), ranging from 22 to 65 years old, who underwent an orthotopic liver transplantation (OLT) at the Hospital Universitario Central de Asturias (HUCA) between 2007 and 2010.

Methods: The main objective was to evaluate the post-OLT critical complication prognosis usefulness of the precursors of three new biomarkers: mid-regional proadrenomedullin (MR-proADM), carboxy-terminal-proendothelin-1 (CT-ProET-1) and mid-regional proatrial natriuretic peptide (MR-ProANP). As all of them are blood pressure mediators, stress-associated physiological phenomena are expected to affect their expression and secretion, mainly those related to blood circulation. Therefore, as a second goal, the biological variability of the biomarkers has been studied in a set of OLT patients without complications during the first postoperative week. The knowledge of the reference change value of the new biomarkers will be interesting for their correct interpretation in future investigations. The prognostic value of the new biomarkers was also compared to that of procalcitonin (PCT).

Results: It has been shown that the basal concentration of the biomarkers is higher in patients that undergo OLT than in the normal population, correlating with the severity of the pathology. The intra-individual biological variation of these biomarkers is similar to other biochemical parameters, the reference change value for OLT patients being 90% for CT-proET-1, 112% for MR-proADM and 127% for MR-proANP.

Conclusions: Multivariate analysis showed that MR-proADM was the best biomarker for the prognosis of severe complications.
50th anniversary of Clinical Chemistry and Laboratory Medicine – a historical overview1)
Authors: Körber Friedrich , Plebani Mario 
Published: December 2012

Abstract
In the early 1960s, Joachim Brugsch, one of the founders of Clinical Chemistry and Laboratory Medicine (CCLM) (then Zeitschrift für Klinische Chemie), had the idea to found a journal in the upcoming field of clinical chemistry. He approached Ernst Schütte, who was associated with the De Gruyter publishing house through another journal, to participate, and Schütte thus became the second founder of this Journal. The aim was to create a vehicle allowing the experts to express their opinions and raise their voices more clearly than they could in a journal that publishes only original experimental papers, a laborious and difficult, but important endeavor, as the profession of clinical chemistry was still in the early stages of development at this time. The first issue of this Journal was published in early 1963, and today, we are proud to celebrate the 50th anniversary of CCLM. This review describes the development of this Journal in light of the political situation of the time when it was founded, the situation of the publisher Walter De Gruyter after the erection of the Berlin Wall, and the development of clinical chemistry, and later on, laboratory medicine as a well-acknowledged discipline and profession.
Synthesis and antioxidant activity of a series of novel 3-chalcone-substituted 1,4-dihydropyridine derivatives
Authors: Sun Hao , Shang Chengxiang , Jin Longfei , Zhang Jian 
Published: December 2012

Abstract
New 3-chalcone-substituted 1,4-dihydropyridine (DHP) derivatives have been synthesized based on dimethyl or diethyl 2,6-dimethyl-4-phenyl-1,4-DHP-3,5-dicarboxylate. Their structures were confirmed by IR, 1H NMR, 13C NMR, and elemental analyses. The synthesized compounds were also screened for antioxidant properties.
Focused microwave-assisted efficient and convenient synthesis of new pyrido[2,3-d]pyrimidinone derivatives
Authors: Quan Zheng-Jun , Liang Jun-Ling , Bai Lin , Zhang Zhang , Da Yu-Xia , Wang Xi-Cun 
Published: December 2012

Abstract
A rapid and efficient method for the preparation of new pyrido[2,3-d]pyrimidinone derivatives by the condensation of 2-amino-4-aryl-6-arylnicotinamides with carbon disulfide or cycloalkanones under focused microwave irradiation is described.
Homo- and heterotypic interactions between Pss proteins involved in the exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii
Authors: Marczak Małgorzata , Dźwierzyńska Monika , Skorupska Anna 
Published: December 2012

Abstract

Rhizobium leguminosarum produces large amounts of exopolysaccharide (EPS) that has been shown to be an important determinant of successful nitrogen-fixing symbiosis with legume plants. EPS is assembled in a Wzx/Wzy-dependent manner, and proteins involved in the process are proposed to form a complex that enables coupling the synthesis of EPS subunits with their polymerization and transport. Pss proteins, which are encoded within the chromosomal polysaccharide synthesis cluster of Rhizobium leguminosarum bv. trifolii TA1, were subjected to interaction analysis. PssN was shown to form multimeric complexes in the outer membrane and interact with the extracellular PssO protein and the inner membrane oligomeric PssP co-polymerase. PssO was demonstrated to form oligomers in the presence of the cross-linker. Bacterial two-hybrid analysis showed that PssP interacts with PssL and PssT, counterparts of Gram-negative bacteria Wzx and Wzy proteins. Membrane topology of PssT is discussed in the context of its plausible Wzy-like polymerase activity, interactions with PssP and a possible impact of these interactions on EPS polymerization and chain length determination. The importance of protein-protein and putative protein-polysaccharide interactions in EPS transport is discussed. A topology model for the EPS transport system, with highlights on localization, functions and interactions between the Pss proteins, is proposed.
HIF mediated and DNA damage independent histone H2AX phosphorylation in chronic hypoxia
Authors: Wrann Simon , Kaufmann Muriel R. , Wirthner Renato , Stiehl Daniel P. , Wenger Roland H. 
Published: December 2012

Abstract
The histone variant 2AX (H2AX) is phosphorylated at Serine 139 by the PI3K-like kinase family members ATM, ATR and DNA-PK. Genotoxic stress, such as tumor radio- and chemotherapy, is considered to be the main inducer of phosphorylated H2AX (γH2AX), which forms distinct foci at sites of DNA damage where DNA repair factors accumulate. γH2AX accumulation under severe hypoxic/anoxic (0.02% oxygen) conditions has recently been reported to follow replication fork stalling in the absence of detectable DNA damage. In this study, we found HIF-dependent accumulation of γH2AX in several cancer cell lines and mouse embryonic fibroblasts exposed to physiologically relevant chronic hypoxia (0.2% oxygen), which did not induce detectable levels of DNA strand breaks. The hypoxic accumulation of γH2AX was delayed by the RNAi-mediated knockdown of HIF-1α or HIF-2α and further decreased when both HIF-αs were absent. Conversely, basal phosphorylation of H2AX was increased in cells with constitutively stabilized HIF-2α. These results suggest that both HIF-1 and HIF-2 are involved in γH2AX accumulation by tumor hypoxia, which might increase a cancer cell’s capacity to repair DNA damage, contributing to tumor therapy resistance.
Synthesis of Different Morphologies of PbS Nanostructures via Hydrothermal Process
Authors: Ghanbari Davood , Salavati-Niasari Masoud , Davar Fatemeh 
Published: December 2012

AbstractLead sulfide nanostructures with different morphologies and particle sizes were obtained via a simple hydrothermal reaction between lead salicylate (Pb(Hsal)2) and thioglycolic acid The novelty in this synthesis is using of thioglycolic acid as a sulfur source and stability agent simultaneously. Depending on the pH of the solution, morphologies of the PbS varied and crystals with shapes of nanoparticles, flower-like nanostructures and microrods were obtained. The effect of the reaction time on the morphology of the nanostructures has been investigated. Products were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) ultraviolet-visible (UV-vis) and Fourier transform infrared (FT-IR) spectroscopy.
Calculation of the Raman Linewidths of Lattice Modes Close to the α-β Transition in Quartz
Authors: Lider Mustafa Cem , Yurtseven Hamit 
Published: December 2012

AbstractThe Raman frequencies of the lattice modes (147 cm−1 and 207 cm−1) are analyzed for the α-β transition in quartz according to a power-law formula with the critical exponent by using the experimental data. The temperature dependence of the Raman frequency is associated with the order parameter (polarization P) for this transition in the quartz crystal.The damping constant of the lattice modes studied here is calculated using the Raman frequencies at various temperatures for the α-β transition in quartz (Tc = 846 K) using the soft mode – hard mode and the energy fluctuation models. Our calculations for the damping constant (bandwidths) give an evidence that the lattice mode of the 147 cm−1 exhibits a soft mode behavior for the α-β transition in quartz.
Production and Characterization of Hydroxyapatite-Zirconia Composites
Authors: Ozmen Melis , Akin Ipek , Marsoglu Muzeyyen 
Published: December 2012

AbstractHydroxyapatite (HA) ceramics are used extensively in different medical applications, such as biomaterial for repair or replacement of bone tissues since it resembles mineral component of bone and teeth. However, HA exhibits low fracture toughness due to its lack of strength and brittleness, thereby providing an obstacle to its application in implants that must withstand to high loads. In this study, HA was synthesized from human teeth by using a single calcinations method. Hydroxyapatite powders were mixed with different amounts of zirconia. The composites were sintered at different temperatures and characterized in terms of mechanical properties and the optimum sintering temperature was determined for good mechanical properties.
Thermodynamic Modeling of the CaO-SiO2-M2O (M=K,Na) Systems
Authors: Zhang Ligang , Schmetterer Clemens , Masset Patrick J. 
Published: December 2012

AbstractThe aim of the present study is the CALPHAD modeling of the systems CaO-SiO2-M2O (M=K,Na) based on a careful review of the available literature data – phase diagram and thermodynamics – as well as own experiments. The heat capacities (Cp) of three compounds, CaSiO3, K2Ca2Si2O7 and K8CaSi10O25 (determined using drop calorimetry), were included in the optimization of the ternary phase diagram CaO-SiO2-K2O.
Short- and medium-term impact of a residential weight-loss camp for overweight adolescents
Authors: Fonseca Helena , Palmeira Antonio Labisa , Martins Sandra , Ferreira Pedro Dias 
Published: December 2012

Abstract
Introduction: Management of adolescent obesity in clinical practice has been usually centered on the promotion of healthy eating and an active lifestyle, with a weak evidence base. In search of creative and more effective interventions, our objective was to evaluate the immediate and follow-up effectiveness of a residential camp program for obese adolescents.
Methods: A total of 28 obese adolescents (15 girls), mean age 13.3±1.7 years and body mass index (BMI) 30.3±5.4 kg/m2, who were attending a tertiary health unit, enrolled in a 2-week camp. The activities were prepared and supervised by different specialists. All adolescents were assessed for anthropometric and psychosocial variables including body image, self-esteem, quality of life and depressive symptoms. Evaluations were conducted on the first and last day of the camp and at 6 months’ follow-up. The effect of treatment was analyzed using analysis of variance (ANOVA) mixed models.
Results: At the end of the camp, weight was reduced by –2.7±1.2%, BMI by –0.8±0.4 units and waist circumference by –2.8±9.8 cm. A reduction in depressive symptoms was identified, with greater reductions in BMI associated with a more significant improvement in depressive symptoms. At the 6 months’ follow-up only girls maintained BMI reduction, with boys having increased their BMI.
Discussion: In the short-term the camp was effective in reducing BMI and improving depressive symptoms. Only girls were able to proceed with BMI reduction at 6 months. These gender differences should be further explored. Ongoing research is examining the maintenance of these improvements.
CSF leaks after cranial surgery — a prospective multicenter analysis
Authors: Kehler Uwe , Hirdes Carsten , Weber Clemens , Spuck Sebastian , Tronnier Volker , Kundt Guenther , Piek Jürgen 
Published: December 2012

Abstract

Background: Cerebrospinal fluid (CSF) leaks are well-known and frequent complications of intracranial procedures. Numerous studies have focused on the clinical aspects of incidence, risk factors, outcome, treatment, and prevention; however, large prospective multicenter studies are missing. The aim of this study was to prospectively analyze the rate of CSF leaks and their causes.

Method: A total of 545 patients with a variety of intracranial procedures (elective and trauma) were recruited in a multicenter, prospective, observational study over a 12-month period.

Results: In 545 cranial surgeries, we observed a CSF leak rate of 7.7% (n=42) at the time of discharge from the hospital. Significant risk factors for CSF leaks were posterior fossa surgery, opened pneumatized spaces, patients younger than 66 years, size of craniotomies, craniectomies rather than craniotomies, remaining dura defects larger than 1 cm, and wound closure without using muscle sutures, continuous locked, or unlocked sutures. Non-significant risk factors for CSF leaks were revision craniotomies, craniotomies for different pathologies, previous radiotherapy and/or systemic chemotherapy, augmentation of dura sutures with various materials, and wound drains as well as temporary CSF drains.

Conclusion: Despite the number of techniques and developments for dural closure, the problem of CSF leaks remains evident and further improvement has to be made.
Certain problems of synchronization theory
Authors: Aisagaliev Serikbai A. , Kalimoldayev Maxat N. 
Published: December 2012

Abstract.
We investigate the global asymptotical stability of dynamical systems with cylindrical phase space, which appear in the synchronization problem of various control systems. New effective criteria of the global asymptotic stability of a countable equilibrium position are proposed. The technique of improper integrals evaluation along solutions is used.
Isolated low-normal amniotic fluid volume in the early third trimester: association with adverse perinatal outcomesa
Authors: Hashimoto Kazumasa , Kasdaglis Tania , Jain Sheveta , Atkins Kristin , Harman Chris R. , Baschat Ahmet A. 
Published: December 2012

Abstract

Aims: To test if an isolated finding of low-normal amniotic fluid index (AFI) in the early third trimester in low-risk patients is associated with adverse perinatal outcomes.

Methods: Retrospective cohort study with uncomplicated singleton pregnancies that had ultrasound studies between 28.0 and 31.9 weeks’ gestation. Two cohorts with AFI 8.0–11.9 cm (low-normal, LN) and AFI 12.0–19.9 cm (mid-normal, MN) were compared.

Results: Patients with LN-AFI (n=99) were more likely to have early (<34 weeks) and late (<37 weeks) preterm birth (PTB) (relative risk 4.2 and 2.4, respectively) and a small for gestational age (SGA) infant (relative risk 1.8) than MN-AFI (n=834), corresponding to a higher NICU admission rate (relative risk 2.5). The risk of “spontaneous” PTB (preterm labor and rupture of membranes) did not differ between the cohorts, whereas the risk of “indicated” PTB (maternal or fetal indications) was significantly increased in LN-AFI. The incidence of abnormal antepartum testing, stillbirth, preeclampsia, placental abruption, fetal intolerance to labor, emergency cesarean delivery, umbilical artery pH <7.0, Apgar scores <7 at 5 min, and neonatal death was not increased in patients with LN-AFI.

Conclusion: Low-normal AFI in the early third trimester increases the risk for subsequent delivery of an SGA infant and indicated PTB.
Presence of human milk bank is associated with elevated rate of exclusive breastfeeding in VLBW infants
Authors: Arslanoglu Sertac , Moro Guido E. , Bellù Roberto , Turoli Daniela , De Nisi Giuseppe , Tonetto Paola , Bertino Enrico 
Published: December 2012

Abstract
Human milk confers health benefits of vital importance for the sick and preterm infants in neonatal intensive care units (NICUs). Mother’s own milk is the first choice in preterm infant feeding, and every effort should be made to promote lactation. When mother’s milk is not available or is insufficient, donor human milk (DHM) is recommended. Yet, occasionally, the concern that the use of DHM might decrease breastfeeding is being raised. The present data collection planned by the Italian Association of Human Milk Banks (AIBLUD) in collaboration with the Italian Neonatal Network (INN) attempted to address this concern. A total of 4277 very low birth weight (VLBW) infants from 83 Italian NICUs were evaluated for this comparative analysis. The 83 Italian NICUs were divided into two groups: centers with a human milk bank (HMB) and centers without a HMB; the available parameters in the network – “any and exclusive breastfeeding rates” and “exclusive formula rate” at discharge – were compared. Exclusive breastfeeding rate at discharge was significantly higher in NICUs with a HMB than in NICUs without (29.6% vs. 16.0%, respectively). Any breastfeeding rate at discharge tended to be higher in the NICUs with HMB (60.4% vs. 52.8%, P=0.09), and exclusive formula rate was lower in the NICUs with HMB (26.5% vs. 31.3%), but this difference was not significant. This report shows that the presence of a HMB and the use of DHM in NICU are associated with increased breastfeeding rate at discharge from the hospital for VLBW infants.