bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards dynamic decision-making in government as service organization: insights from systems thinking
Authors: Carrubbo Luca , Cosimato Silvia , Gagliardi Anna Roberta 
Published: July 2024

Purpose
Service organizations operate in an increasingly complex and uncertain context that makes decision-making challenging. Despite well-recognized changes in the operational context of government as service organization, service literature has given surprisingly limited attention to what these changes imply for organizational decision-making. This study aims to face with the lack of fit of decision-making theorizing with the reality, within which most service practitioners operate, in order to foster the relevance of decision-making in service research and properly approach the false assumptions and misguided instructions for action.

Design/methodology/approach
To rectify the situation, the purpose of this paper is to advance a more holistic understanding of decision-making in government as service organization. The authors do so by reviewing the sparse, though insightful, prior literature on decision-making in service research and identifying four foundational assumptions of decision-making in the service context, that radically differ from the traditional assumptions of decision-making within the wider management literature.

Findings
The authors contribute to service research by further advancing the emerging dynamic understanding of decision-making by developing eight systems thinking-informed research propositions and a connected research agenda. In doing so, the paper offers the essential ground work that can revitalize the field of service management and equip it for facing the challenges that government as service organization is encountering in the 21st century.

Originality/value
The formulated eight research propositions demonstrate that decision-making in a government as service organization occurs within complex adaptive systems composed of multiple subsystems and is characterized by a high degree of unpredictability. It is a process influenced by multiple actors part of the system and subsystems, through multiple feedback loops, where the implications of prior decisions inform the future decisions.
Using the children’s accelerated trauma technique with adults with intellectual disabilities
Authors: O'Farrell Kevin , Garner Nick , Symes Matt 
Published: July 2024

Purpose
This paper aims to explore using the children’s accelerated trauma technique (CATT) in an adult intellectual disability population, with the aim of reducing symptoms of post-traumatic stress disorder (PTSD).

Design/methodology/approach
A service evaluation was completed to investigate differences between pre- and post-CATT trauma scores.

Findings
Scores on a measure of PTSD decreased significantly following CATT.

Practical implications
The findings support continued use of CATT in adults with intellectual disabilities, and they highlight the need to evaluate effectiveness in a larger sample.

Originality/value
This paper evaluates the novel application of a trauma treatment – developed for use with children – in an adult intellectual disability population and adds to the existing evidence base.
Dystrophic epidermolysis bullosa characterized by mucosal lesions in a Chinese familial case with a novel compound heterozygous mutation of COL7A1
Authors: Lan Qingying , Hua Hong , Zhou Peiru 
Published: July 2024

AbstractDystrophic epidermolysis bullosa (DEB) is a rare, but severe, subtype of epidermolysis bullosa. It is characterized mainly by blisters and miliary rashes of the skin, while oral mucosa‐dominated cases are extremely rare. Here, we report the characteristics of oral mucosa lesions in a Chinese familial case of DEB with a novel compound heterozygous COL7A1 mutation. We further analyzed the genetic and molecular features of the proband and the two related mutation carriers. Our study further elucidates the genetic and phenotypic heterogeneity of DEB.
Salvage logging and subsequent post‐windthrow management diminish forest bird communities for two decades
Authors: Walesiak Michał , Michielsen Rosanne , Mikusiński Grzegorz , Żmihorski Michał 
Published: July 2024

Abstract

Post‐disturbance forest management is known to impair biodiversity, including bird communities, but most studies focus on short‐term effects of salvage logging, without recognition of the cumulative, lasting impact of collective post‐disturbance silvicultural practices.

We tracked bird community succession in a temperate pine forest from 5 to 19 years after wind disturbance in managed and unmanaged windthrow areas, covering the period from gap creation to early canopy regeneration and comparing it to nearby production forest unaffected by wind disturbance.

Bird communities of unmanaged windthrow were consistently the richest in terms of bird abundance and richness. They hosted comparable forest bird diversity to unaffected production stands throughout the study period, as well as a substantial diversity of farmland birds.

Managed and unmanaged windthrows followed two distinct successional pathways and did not converge. Despite the bird communities in both windthrows gradually resembling those found in undisturbed forests as the canopy regenerated, each type of windthrow and the unaffected production forest maintained distinct communities until the end of the study.

Synthesis and applications. Restraining from any active post‐windthrow management sets bird succession on a distinct pathway, leading to unique and diverse bird communities. We advocate viewing natural disturbances as cost‐effective ecosystem restoration tools, provided no active management is implemented. Furthermore, we advise against using the term ‘salvage logging’ to refer to collective post‐disturbance silvicultural practices, as its varying interpretation may lead to growing confusion as the number of long‐term studies increase.
Quercetin‐4′‐O‐β‐D‐glucopyranoside inhibits podocyte injury by SIRT5‐mediated desuccinylation of NEK7
Authors: Wu Menghua , Ye Xiaoli 
Published: July 2024

AbstractDiabetic kidney disease (DKD) is a complication of diabetic mellitus. New treatments need to be developed. This study aimed to investigate the effects of quercetin‐4′‐O‐β‐D‐glucopyranoside (QODG) on podocyte injury. Podocytes were cultured in high glucose (HG) medium, treated with QODG, and overexpressing or knocking down SIRT5. Oxidative stress indicators were assessed using corresponding kits. Pyroptosis was detected by flow cytometry and western blot analysis. Succinylation modification was detected using immunoprecipitation (IP) and western blot analysis. The interaction between NEK7 and NLRP3 was determined by co‐IP. The results indicated that QODG inhibited oxidative stress and pyroptosis of podocytes induced by HG. Besides, QODG suppressed succinylation levels in HG‐induced podocytes, with the upregulation of SIRT5. Knockdown of SIRT5 reversed the effects of QODG on oxidative stress and pyroptosis. Moreover, SIRT5 inhibited the succinylation of NEK7 and the interaction between NLRP3 and NEK7. In conclusion, QODG upregulates SIRT5 to inhibit the succinylation modification of NEK7, impedes the interaction between NEK7 and NLRP3, and then inhibits the pyroptosis and oxidative stress injury of podocytes under HG conditions. The findings suggested that QODG has the potential to treat DKD and explore a novel underlying mechanism of QODG function.
Comprehensive genetic analysis reveals the genetic structure and diversity of Calanthe hoshii (Orchidaceae), an endemic species of the Ogasawara Islands: Implications for appropriate conservation of a critically endangered species
Authors: Katafuchi Mayu , Narita Satoshi , Komaki Yoshiteru , Nagano Atsushi J. , Yukawa Tomohisa , Suyama Yoshihisa , Hirota Shun K. , Yamasaki Michimasa , Isagi Yuji 
Published: July 2024

AbstractThe Ogasawara Islands, isolated from the continent throughout their geological history, harbor abundant endemic species. However, 32% of the native plant species are on the Japanese Red List, signaling their endangered status. Among these endangered species, Calanthe hoshii stands out as highly susceptible to extinction. Two wild and 89 ex situ individuals were alive in 2020, but the last known wild individual died in October 2021, indicating this species may have become extinct in the wild. Despite the conservation efforts under the Act for the Conservation of Endangered Species of Wild Fauna and Flora, the lack of essential information regarding genetic diversity and population structure among the remaining individuals poses a significant obstacle to developing effective conservation measures. In this research, we conducted a genetic analysis of the wild and ex situ individuals, which revealed notably reduced genetic diversity between individuals, with individual heterozygosity levels (1.0 × 10−5) measuring one‐ninth of those observed within a closely related species Calanthe triplicata (1.5 × 10−4). Comprehensive genetic analysis revealed that C. hoshii consisted of three genetic clusters of different sizes: cluster 1 comprised 95% of the total population, while clusters 2 and 3, with few individuals, were only found in the ex situ populations at Koishikawa Botanical Garden. Since extant C. hoshii maintains a remarkably low genetic diversity at both individual and population levels, it is necessary to consider future management strategies, such as artificial breeding among different clades identified in this study, to safeguard the stability and resilience of this species.
Genome‐based taxonomic analysis of the genus Pseudoalteromonas reveals heterotypic synonyms
Authors: Videau Patrick , Shlafstein Maximillian D. , Oline David K. , Givan Scott A. , Chapman Linda Fleet , Strangman Wendy K. , Hahnke Richard L. , Saw Jimmy H. , Ushijima Blake 
Published: July 2024

AbstractThe Pseudoalteromonas genus comprises members that have been demonstrated to play significant ecological roles and produce enzymes, natural products, and activities that are beneficial to the environment and economy. A comprehensive evaluation of the genus revealed that the genomes of several Pseudoalteromonas species are highly similar to each other, exceeding species cutoff values. This evaluation involved determining and comparing the average nucleotide identity, in silico DNA–DNA hybridization, average amino acid identity, and the difference in G + C% between Pseudoalteromonas type strains with publicly available genomes. The genome of the Pseudoalteromonas elyakovii type strain was further assessed through additional sequencing and genomic comparisons to historical sequences. These findings suggest that six Pseudoalteromonas species, namely P. mariniglutinosa, P. donghaensis, P. maricaloris, P. elyakovii, P. profundi, and P. issachenkonii, should be reclassified as later heterotypic synonyms of the following validly published species: P. haloplanktis, P. lipolytica, P. flavipulchra, P. distincta, P. gelatinilytica, and P. tetraodonis. Furthermore, two names without valid standing, ‘P. telluritireducens’ and ‘P. spiralis’, should be associated with the validly published Pseudoalteromonas species P. agarivorans and P. tetraodonis, respectively.
When the going gets tough, the tough get going—Novel bacterial AAA+ disaggregases provide extreme heat resistance
Authors: Bohl Valentin , Mogk Axel 
Published: July 2024

AbstractHeat stress can lead to protein misfolding and aggregation, potentially causing cell death due to the loss of essential proteins. Bacteria, being particularly exposed to environmental stress, are equipped with disaggregases that rescue these aggregated proteins. The bacterial Hsp70 chaperone DnaK and the ATPase associated with diverse cellular activities protein ClpB form the canonical disaggregase in bacteria. While this combination operates effectively during physiological heat stress, it is ineffective against massive aggregation caused by temperature‐based sterilization protocols used in the food industry and clinics. This leaves bacteria unprotected against these thermal processes. However, bacteria that can withstand extreme, man‐made stress conditions have emerged. These bacteria possess novel ATPase associated with diverse cellular activities disaggregases, ClpG and ClpL, which are key players in extreme heat resistance. These disaggregases, present in selected Gram‐negative or Gram‐positive bacteria, respectively, function superiorly by exhibiting increased thermal stability and enhanced threading power compared to DnaK/ClpB. This enables ClpG and ClpL to operate at extreme temperatures and process large and tight protein aggregates, thereby contributing to heat resistance. The genes for ClpG and ClpL are often encoded on mobile genomic islands or conjugative plasmids, allowing for their rapid spread among bacteria via horizontal gene transfer. This threatens the efficiency of sterilization protocols. In this review, we describe the various bacterial disaggregases identified to date, characterizing their commonalities and the specific features that enable these novel disaggregases to provide stress protection against extreme stress conditions.
Clinicians' experiences and perceptions of family therapy in the context of autism
Authors: Hsiang Cindy H. T. , Campbell Linda , Tavener Meredith 
Published: July 2024

AbstractThere is a paradoxical paradigm operating in neurodiverse families with autistic children, indicating increased stress alongside enrichment of family life. An emerging body of research has shown that the use of family therapy can improve family functioning and relationships in neurodiverse families. The current study uses constructivist grounded theory to explore clinicians' experiences and perceptions of utilising family therapy in the context of autism. Study findings suggest that clinicians integrate therapeutic techniques from diverse family therapy approaches to improve family relations by bridging different ways of learning and communicating as well as facilitating multiple perspectives. Integrating therapeutic techniques across diverse approaches further encompasses working with the systems around the individual with autism. To adapt family therapy in the context of autism, it is suggested that clinicians examine their own perspectives and beliefs about autism and keep a curious stance during the process.
Policy Recommendations for Coordinated and Sustainable Growth of the Behavioral Health Workforce
Authors: LAST BRIANA S. , CRABLE ERIKA L. 
Published: July 2024

Policy Points
Demand for behavioral health services outpaces the capacity of the existing workforce, and the unmet need for behavioral health services is expected to grow.
This paper summarizes research and policy evidence demonstrating that the long‐standing challenges that impede behavioral health workforce development and retention (i.e., low wages, high workloads, training gaps) are being replicated by growing efforts to expand the workforce through task‐sharing delivery to nonspecialist behavioral health providers (e.g., peer specialists, promotores de salud).
In this paper, we describe policy opportunities to sustain behavioral health workforce growth to meet demand while supporting fair wages, labor protections, and rigorous training.
Alcohol‐Specific Inpatient Diagnoses in Germany: A Retrospective Cross‐Sectional Analysis of Primary and Secondary Diagnoses from 2012 to 2021
Authors: Manthey Jakob , Jacobsen Britta , Kilian Carolin , Kraus Ludwig , Reimer Jens , Schäfer Ingo , Schulte Bernd 
Published: July 2024

AbstractAimsOur study aimed to a) describe the distribution of hospital discharges with primary and secondary alcohol‐specific diagnoses by sex and age group, and b) describe how the number of hospital discharges with primary and secondary alcohol‐specific diagnoses have changed across different diagnostic groups (categorized by primary International Classification of Diseases, 10th Revision [ICD‐10] diagnosis) over time.DesignRetrospective cross‐sectional analysis.SettingGerman hospital settings between 2012 and 2021.ParticipantsAll persons aged 15–69 admitted to hospitals as registered in a nationwide data set.MeasurementsWe counted a) the number of all hospital discharges and b) the number of hospital discharges with at least one alcohol‐specific secondary diagnosis (secondary alcohol‐specific diagnosis) by year, sex, age group, and diagnostic group. One diagnostic group included all primary alcohol‐specific diagnoses, while 13 additional groups aligned with ICD‐10 chapters (e.g., neoplasms). Alcohol‐involvement was defined as either a primary or secondary alcohol‐specific diagnosis.FindingsOf 95 417 204 recorded hospital discharges between 2012 and 2021, 3 828 917 discharges (4.0%; 2 913 903 men (6.4%); 915 014 women (1.8%)) involved either a primary or at least one secondary diagnosis related to alcohol. Of all alcohol‐involved hospital discharges, 56.8% (1 654 736 discharges) had no primary but only a secondary alcohol‐specific diagnosis. Secondary alcohol‐specific diagnoses were particularly prevalent in hospital discharges due to injuries. With rising age, alcohol‐involvement in hospital discharges due to digestive or cardiovascular diseases increased. Between 2012 and 2021, the rate of alcohol‐involved hospital discharges has decreased more in younger as compared with older adults (average change between 2012 and 2021: 15–24: −55%; 25–34: −41%; 35–44: −23%; 45–54: −31%; 55–64: −21%; 65–69: −8%).ConclusionsThe number of alcohol‐involved hospital discharges in Germany from 2012 to 2021 more than doubles (from 1 654 736 to 3 828 917) when including secondary alcohol‐specific diagnoses. More pronounced declines among younger adults may be attributed to unequal changes in alcohol consumption patterns across the population and to the hazardous effects of long‐term alcohol use.
Peri‐operative care of transgender and gender‐diverse individuals: guidance for clinicians and departments
Authors: Edwardson Stuart , Flower Luke , Fawcett Erik , Medlock Rebecca , Cheung Ada S. , Kamaruddin Kamilla , McCormack Victoria L. , Agarwal Seema 
Published: July 2024

SummaryIntroductionThe objective of this document is to guide best practice to ensure the safety and dignity of transgender and gender‐diverse people in the peri‐operative period. While transgender and gender‐diverse people may have specific health needs in relation to gender dysphoria, their health requirements go beyond their gender identity. Most doctors will provide care to someone who is transgender or gender‐diverse at some stage in their career. It is therefore important that all anaesthetists are educated on specific considerations when caring for these patients.MethodsA working party was assembled consisting of individuals with experience in direct clinical care of the relevant patient group, those who have expertise in endocrinology and gender‐affirming hormones, educators on the topic of transgender and gender‐diverse healthcare, and authors of both cisgender and transgender identities. After discussion among the working party, targeted searches of literature were undertaken.ResultsThe authors initially came up with a list of over 25 recommendations which was subsequently revised to a list of 15 recommendations after further review by the working party. These included airway assessment and management; management of hormonal therapy; relevant issues in obstetric anaesthesia; and hospital infrastructure and processes.ConclusionsThis document provides the first guidance produced to advise on best practice to ensure the safety and dignity of trans and gender‐diverse individuals in the peri‐operative period.
High‐intensity interval training with functional electrical stimulation cycling for incomplete spinal cord injury patients: A pilot feasibility study
Authors: Manaf Haidzir , Hamzaid Nur Azah , Hasnan Nazirah , Yiwei Chen , Mohafez Hamidreza , Hisham Hafifi , Davis Glen 
Published: July 2024

AbstractBackgroundConsequences of spinal cord injury (SCI) with a sedentary lifestyle will progress to muscle weakness and muscle atrophy, leading to muscle fatigue. This study aimed to determine the feasibility and preliminary effects of high‐intensity interval training (HIIT) using functional electrical stimulation (FES) cycling on changes in thigh muscle volume and muscle strength, in patients with incomplete SCI.MethodsEight incomplete SCI patients (mean age 50 years; 6 women) with stable SCI paraplegia (mean 6.75 years since injury) participated in the HIIT FES cycling (85%–90% peak Watts; 4 × 4–min intervals) three times a week (over 6 weeks). The main outcomes were adherence, participant acceptability, and adverse events. Secondary outcomes were muscle strength (peak torque) and leg volume changes.ResultsOur findings revealed that the program was well‐received by participants, with high levels of adherence, positive feedback, and satisfaction, suggesting that it could be a promising option for individuals seeking to enhance their lower body strength and muscle mass. Additionally, all participants successfully completed the training without any serious adverse events, indicating that the program is safe for use. Finally, we found that the 6‐week HIIT FES leg cycling exercise program resulted in notable improvements in isometric peak torque of the quadriceps (range 13.9%–25.6%), hamstring muscle (18.2%–23.3%), and leg volume (1.7%–18.2%).ConclusionsThis study highlights HIIT FES leg cycling exercise program potential as an effective intervention for improving lower limb muscle function.
Evaluation of cell survival in different 3D‐printed geometric shapes of human iPSC‐derived engineered heart tissue
Authors: Yildirim Yalin , Degener Louisa , Reuter Lukas , Petersen Johannes , Gabel Lilian , Sommer Annika , Pahrmann Christiane , Reichenspurner Hermann , Pecha Simon 
Published: July 2024

AbstractObjectivesEngineered Heart Tissue (EHT) is a promising tool to repair heart muscle defects and can additionally be used for drug testing. Due to the absence of an in vitro vascularization, EHT geometry crucially impacts nutrient and oxygen supply by diffusion capacity. We analyzed cardiomyocyte survival in different EHT geometries.MethodsDifferent geometries with varying surface‐area‐to‐volume‐ratios were calculated (structure A (Ring) AS/V = 58.47 mm2/440 μL3, structure B (Infinity) 25.86 mm2/440 μL3). EHTs were generated from hiPSC‐derived cardiomyocytes (4 × 106) and a fibrin/thrombin hydrogel. Cell viability was evaluated by RT‐PCR, cytometric studies, and Bioluminescence imaging.ResultsUsing 3D‐printed casting molds, spontaneously beating EHTs can be generated in various geometric forms. At day 7, the RT‐PCR analyses showed a significantly higher Troponin‐T value in ring EHTs, compared to infinity EHTs. In cytometric studies, we evaluated 15% more Troponin‐T positive cells in ring (73% ± 12%), compared to infinity EHTs (58% ± 11%, p = 0.04). BLI visualized significantly higher cell survival in ring EHTs (ROI = A: 1.14 × 106 p/s and B: 8.47 × 105 p/s, p < 0.001) compared to infinity EHTs during longitudinal cultivation process.ConclusionUse of 3D‐printing allows the creation of EHTs in all desired geometric shapes. The geometry with an optimized surface‐area‐to‐volume‐ratio (ring EHT) demonstrated a significantly higher cell survival measured by RT‐PCR, Bioluminescence imaging, and cytometric studies using FACS analysis.