bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrophysiological properties of laryngeal motoneurones in rats submitted to chronic intermittent hypoxia
Authors: Moraes Davi J. A. , Machado Benedito H. 
Published: November 2014

Key points
The respiratory control of the glottis by laryngeal motoneurones is characterized by inspiratory abduction and post‐inspiratory adduction causing decreases and increases in upper airway resistance, respectively.
Chronic intermittent hypoxia (CIH), an important component of obstructive sleep apnoea, exaggerated glottal abduction (before inspiration), associated with active expiration and decreased glottal adduction during post‐inspiration.
CIH increased the inspiratory and decreased the post‐inspiratory laryngeal motoneurone activities, which is not associated to changes in their intrinsic electrophysiological properties.
We conclude that the changes in the respiratory network after CIH seem to be an adaptive process required for an appropriated pulmonary ventilation and control of upper airway resistance under intermittent episodes of hypoxia.
AbstractTo keep an appropriate airflow to and from the lungs under physiological conditions a precise neural co‐ordination of the upper airway resistance by laryngeal motoneurones in the nucleus ambiguus is essential. Chronic intermittent hypoxia (CIH), an important component of obstructive sleep apnoea, may alter these fine mechanisms. Here, using nerve and whole cell patch clamp recordings in in situ preparations of rats we investigated the effects of CIH on the respiratory control of the upper airway resistance, on the electrophysiological properties of laryngeal motoneurones in the nucleus ambiguus, and the role of carotid body (CB) afferents to the brainstem on the underlying mechanisms of these effects. CIH rats exhibited longer pre‐inspiratory and lower post‐inspiratory superior laryngeal nerve activities than control rats. These changes produced exaggerated glottal abduction (before inspiration) and decreased glottal adduction during post‐inspiration, indicating a reduction of upper airway resistance during these respiratory phases after CIH. CB denervation abolished these changes produced by CIH. Regarding choline acetyltransferase positive‐laryngeal motoneurones, CIH increased the firing frequency of inspiratory and decreased the firing frequency of post‐inspiratory laryngeal motoneurones, without changes in their intrinsic electrophysiological properties. These data show that the effects of CIH on the upper airway resistance and laryngeal motoneurones activities are driven by the integrity of CB, which afferents induce changes in the central respiratory generators in the brainstem. These neural changes in the respiratory network seem to be an adaptive process required for an appropriated pulmonary ventilation and control of upper airway resistance under intermittent episodes of hypoxia.
Non‐receptor tyrosine kinases and the actin cytoskeleton in contractile vascular smooth muscle
Authors: Ohanian Jacqueline , Pieri Maria , Ohanian Vasken 
Published: November 2014

AbstractThe contractility of vascular smooth muscle cells within the walls of arteries is regulated by mechanical stresses and vasoactive signals. Transduction of these diverse stimuli into a cellular response occurs through many different mechanisms, one being reorganisation of the actin cytoskeleton. In addition to a structural role in maintaining cellular architecture it is now clear that the actin cytoskeleton of contractile vascular smooth muscle cells is a dynamic structure reacting to changes in the cellular environment. Equally clear is that disrupting the cytoskeleton or interfering with its rearrangement, has profound effects on artery contractility. The actin cytoskeleton associates with dense plaques, also called focal adhesions, at the plasma membrane of smooth muscle cells. Vasoconstrictors and mechanical stress induce remodelling of the focal adhesions, concomitant with cytoskeletal reorganisation. Recent work has shown that non‐receptor tyrosine kinases and tyrosine phosphorylation of focal adhesion proteins such as paxillin and Hic‐5 are important for actin cytoskeleton and focal adhesion remodelling and contraction.
Characterization of Cross-Reactive Norovirus-Specific Monoclonal Antibodies
Authors: Kou Baijun , Crawford Sue E. , Ajami Nadim J. , Czakó Rita , Neill Frederick H. , Tanaka Tomoyuki N. , Kitamoto Noritoshi , Palzkill Timothy G. , Estes Mary K. , Atmar Robert L. 
Published: November 2014

ABSTRACTNoroviruses (NoVs) commonly cause acute gastroenteritis outbreaks. Broadly reactive diagnostic assays are essential for rapid detection of NoV infections. We previously generated a panel of broadly reactive monoclonal antibodies (MAbs). We characterized MAb reactivities by use of virus-like particles (VLPs) from 16 different NoV genotypes (6 from genogroup I [GI], 9 from GII, and 1 from GIV) coating a microtiter plate (direct enzyme-linked immunosorbent assay [ELISA]) and by Western blotting. MAbs were genotype specific or recognized multiple genotypes within a genogroup and between genogroups. We next applied surface plasmon resonance (SPR) analysis to measure MAb dissociation constants (Kd) as a surrogate for binding affinity; aKdlevel of <10 nM was regarded as indicating strong binding. Some MAbs did not interact with the VLPs by SPR analysis. To further assess this lack of MAb-VLP interaction, the MAbs were evaluated for the ability to identify NoV VLPs in a capture ELISA. Those MAbs for which aKdcould not be measured by SPR analysis also failed to capture the NoV VLPs; in contrast, those with a measurableKdgave a positive signal in the capture ELISA. Thus, some broadly cross-reactive epitopes in the VP1 protruding domain may be partially masked on intact particles. One MAb, NV23, was able to detect genogroup I, II, and IV VLPs from 16 genotypes tested by sandwich ELISA, and it successfully detected NoVs in stool samples positive by real-time reverse transcription-PCR when the threshold cycle (CT) value was <31. Biochemical analyses of MAb reactivity, including SPR analysis, identified NV23 as a broadly reactive ligand for application in norovirus diagnostic assays.
Mapping Broadly Reactive Norovirus Genogroup I and II Monoclonal Antibodies
Authors: Crawford Sue E. , Ajami Nadim , Parker Tracy Dewese , Kitamoto Noritoshi , Natori Katsuro , Takeda Naokazu , Tanaka Tomoyuki , Kou Baijun , Atmar Robert L. , Estes Mary K. 
Published: November 2014

ABSTRACTNoroviruses are responsible for most acute nonbacterial epidemic outbreaks of gastroenteritis worldwide. To develop cross-reactive monoclonal antibodies (MAbs) for rapid identification of genogroup I and II (GI and GII) noroviruses (NoVs) in field specimens, mice were immunized with baculovirus-expressed recombinant virus-like particles (VLPs) corresponding to NoVs. Nine MAbs against the capsid protein were identified that detected both GI and GII NoV VLPs. These MAbs were tested in competition enzyme-linked immunosorbent assays (ELISAs) to identify common epitope reactivities to GI and GII VLPs. Patterns of competitive reactivity placed these MAbs into two epitope groups (groups 1 and 2). Epitopes for MAbs NV23 and NS22 (group 1) and MAb F120 (group 2) were mapped to a continuous region in the C-terminal P1 subdomain of the capsid protein. This domain is within regions previously defined to contain cross-reactive epitopes in GI and GII viruses, suggesting that common epitopes are clustered within the P1 domain of the capsid protein. Further characterization in an accompanying paper (B. Kou et al., Clin Vaccine Immunol 22:160–167, 2015,http://dx.doi.org/10.1128/CVI.00519-14) revealed that MAb NV23 (epitope group 1) is able to detect GI and GII viruses in stool. Inclusion of the GI and GII cross-reactive MAb NV23 in antigen detection assays may facilitate the identification of GI and GII human noroviruses in stool samples as causative agents of outbreaks and sporadic cases of gastroenteritis worldwide.
Complete Genome Sequence of Mumps Virus Genotype G from a Vaccinated Child in Franceville, Southeastern Gabon, in 2013
Authors: Maganga Gael D. , Iroungou Berthe A. , Bole-Feysot Christine , Leroy Eric M. , Touré Ndouo Fousseyni S. , Berthet Nicolas 
Published: November 2014

ABSTRACT

The genome of mumps virus (MuV), a member of the family
Paramyxoviridae
of the genus
Rubulavirus
, consists of a single-stranded, negative-sense, nonsegmented RNA. Here, we report the first whole-genome sequence of 15,263 nucleotides of a mumps virus strain from a 6-year-old vaccinated boy in Franceville, southeastern Gabon.
Complete Genome Sequence of
Lelliottia
Podophage phD2B
Authors: Nowicki Grzegorz , Barylski Jakub , Kujawa Natalia , Goździcka-Józefiak Anna 
Published: November 2014

ABSTRACT

The genus
Lelliottia
was recently created from the group of environmental gammaproteobacteria previously included in the genus
Enterobacter
. Here, we report the complete genome sequence of phD2B, the first (according to our current knowledge) known phage that infects bacterium from the taxon.
Complete Genome Sequence of Leuconostoc citreum Strain NRRL B-742
Authors: Passerini Delphine , Vuillemin Marlène , Laguerre Sandrine , Amari Myriam , Loux Valentin , Gabriel Valérie , Robert Hervé , Morel Sandrine , Monsan Pierre , Gabriel Bruno , Fontagné-Faucher Catherine , Remaud-Siméon Magali , Moulis Claire 
Published: November 2014

ABSTRACT

Leuconostoc citreum
belongs to the group of lactic acid bacteria and plays an important role in fermented foods of plant origin. Here, we report the complete genome of the
Leuconostoc citreum
strain NRRL B-742, isolated in 1954 for its capacity to produce dextran.
Draft Genome Sequence of Coxiella burnetii Strain Cb196, an Agent of Endocarditis in Saudi Arabia
Authors: D'Amato Felicetta , Robert Catherine , Azhar E. I. , Fournier Pierre-Edouard , Raoult Didier 
Published: November 2014

ABSTRACTCoxiella burnetiiCb196, with a 2,006,415-bp genome, is a strain isolated from a 45-year-old man in Saudi Arabia with endocarditis. It belongs to the genotype MST51, which was detected for the first time only in this country. Cb196 shows more similarity toC. burnetiiCbuK_Q154, belonging to genotype 8, which was phylogenetically close to MST51.
Draft Genome Sequence of an International Clonal Lineage 1 Acinetobacter baumannii Strain from Argentina
Authors: Vilacoba Elisabet , Déraspe Maxime , Traglia German M. , Roy Paul H. , Ramírez María Soledad , Centrón Daniela 
Published: November 2014

ABSTRACT

In the last few years
Acinetobacter baumannii
has emerged worldwide as an important nosocomial pathogen in medical institutions. Here, we present the draft genome sequence of the international clonal lineage 1 (ICL1)
A. baumannii
strain A144 that was isolated in a hospital in Buenos Aires City in the year 1997. The strain is susceptible to carbapenems and resistant to trimethoprim and gentamicin.
Complete Genome Characterization of Korean Porcine Deltacoronavirus Strain KOR/KNU14-04/2014
Authors: Lee Sunhee , Lee Changhee 
Published: November 2014

ABSTRACT
In April 2014, porcine deltacoronavirus (PDCoV) was first identified in feces from diarrheic piglets in South Korea and found to be closely related to other PDCoV strains. The complete genome of the Korean PDCoV strain, KOR/KNU14-04/2014, was sequenced and analyzed to characterize PDCoV circulating in South Korea.
Complete Genome Sequence of Probiotic Lactobacillus plantarum Strain FMNP01, Isolated from Mango Fruit
Authors: Li Xue-Fei , Liao Xue-Yi , Liu Yong-Feng , Guo Li-Qiong , Ye Zhi-Wei , Lin Jun-Fang 
Published: November 2014

ABSTRACT

Lactobacillus plantarum
strain FMNP01 is a new strain with probiotic properties that was isolated from fresh mango from Guangzhou, China. Here, we report the complete genome of this organism.
Draft Genome Sequence of
Pseudoalteromonas
sp. Strain ND6B, an Oil-Degrading Isolate from Eastern Mediterranean Sea Water Collected at a Depth of 1,210 Meters
Authors: Harris Austin P. , Techtmann Stephen M. , Stelling Savannah C. , Utturkar Sagar M. , Alshibli Noor K. , Brown Steven D. , Hazen Terry C. 
Published: November 2014

ABSTRACT

Here, we report the draft genome of
Pseudoalteromonas
sp. strain ND6B, which is able to grow with crude oil as a carbon source. Strain ND6B was isolated from eastern Mediterranean Sea deep water at a depth of 1,210 m. The genome of strain ND6B provides insight into the oil-degrading ability of the
Pseudoalteromonas
species.
Draft Genome Sequence of Taylorella equigenitalis Strain MCE529, Isolated from a Belgian Warmblood Horse
Authors: Hébert Laurent , Touzain Fabrice , de Boisséson Claire , Breuil Marie-France , Duquesne Fabien , Laugier Claire , Blanchard Yannick , Petry Sandrine 
Published: November 2014

ABSTRACT

Taylorella equigenitalis
is the causative agent of contagious equine metritis (CEM), a sexually transmitted infection of horses. We herein report the genome sequence of
T. equigenitalis
strain MCE529, isolated in 2009 from the urethral fossa of a 15-year-old Belgian Warmblood horse in France.
Genome Sequence of a Multidrug-Resistant Strain of Klebsiella pneumoniae, BAMC 07-18, Isolated from a Combat Injury Wound
Authors: Van Laar Tricia A. , Chen Tsute , Childers Brandon M. , Chen Ping , Abercrombie Johnathan J. , Leung Kai P. 
Published: November 2014

ABSTRACT

Klebsiella pneumoniae
is an important infectious agent of surgical sites and combat wounds. Antibiotic resistance and tolerance are common impediments to the healing of chronic infections. Here, we report the genome sequence of a highly multidrug-resistant strain of
K. pneumoniae
, BAMC 07-18, isolated from a combat wound of a soldier.
Genome Sequence of Corynebacterium ulcerans Strain 210932
Authors: Viana Marcus Vinicius Canário , de Jesus Benevides Leandro , Batista Mariano Diego Cesar , de Souza Rocha Flávia , Bagano Vilas Boas Priscilla Carolinne , Folador Edson Luiz , Pereira Felipe Luiz , Alves Dorella Fernanda , Gomes Leal Carlos Augusto , Fiorini de Carvalho Alex , Silva Artur , de Castro Soares Siomar , Pereira Figueiredo Henrique Cesar , Azevedo Vasco , Guimarães Luis Carlos 
Published: November 2014

ABSTRACT

In this work, we present the complete genome sequence of
Corynebacterium ulcerans
strain 210932, isolated from a human. The species is an emergent pathogen that infects a variety of wild and domesticated animals and humans. It is associated with a growing number of cases of a diphtheria-like disease around the world.
Draft Genome Sequence of the Algicidal Bacterium Mangrovimonas yunxiaonensis Strain LY01
Authors: Li Yi , Zhu Hong , Li Chongping , Zhang Huajun , Chen Zhangran , Zheng Wei , Xu Hong , Zheng Tianling 
Published: November 2014

ABSTRACT

Mangrovimonas yunxiaonensis
LY01, a novel bacterium isolated from mangrove sediment, showed high algicidal effects on harmful algal blooms of
Alexandrium tamarense
. Here, we present the first draft genome sequence of this strain to further understanding of the functional genes related to algicidal activity.
High-Throughput Mycobacterial Interspersed Repetitive-Unit–Variable-Number Tandem-Repeat Genotyping for Mycobacterium tuberculosis Epidemiological Studies
Authors: Gauthier Marie , Bidault Floriane , Mosnier Amandine , Bablishvili Nino , Tukvadze Nestani , Somphavong Silaphet , Paboriboune Phimpha , Ocheretina Oksana , Pape Jean William , Paranhos-Baccala Glaucia , Berland Jean-Luc 
Published: November 2014

ABSTRACT
The emergence of drug-resistant forms of tuberculosis (TB) represents a major public health concern. Understanding the transmission routes of the disease is a key factor for its control and for the implementation of efficient interventions. Mycobacterial interspersed repetitive-unit–variable-number tandem-repeat (MIRU-VNTR) marker typing is a well-described method for lineage identification and transmission tracking. However, the conventional manual genotyping technique is cumbersome and time-consuming and entails many risks for errors, thus hindering its implementation and dissemination. We describe here a new approach using the QIAxcel system, an automated high-throughput capillary electrophoresis system that also carries out allele calling. This automated method was assessed on 1,824 amplicons from 82 TB isolates and tested with sets of markers of 15 or 24 loci. Overall allele-calling concordance between the methods from 140 to 1,317 bp was 98.9%. DNA concentrations and repeatability and reproducibility performances showed no biases in allele calling. Furthermore, turnaround time using this automated system was reduced by 81% compared to the conventional manual agarose gel method. In sum, this new automated method facilitates MIRU-VNTR genotyping and provides reliable results. Therefore, it is well suited for field genotyping. The implementation of this method will help to achieve accurate and cost-effective epidemiological studies, especially in countries with a high prevalence of TB, where the high number of strains complicates the surveillance of circulating lineages and requires efficient interventions to be carried out in an urgent manner.
Design and Evaluation of Peptide Nucleic Acid Probes for Specific Identification of Candida albicans
Authors: Kim Hyun-Joong , Brehm-Stecher Byron F. 
Published: November 2014

ABSTRACT

Candida albicans
is an important cause of systemic fungal infections, and rapid diagnostics for identifying and differentiating
C. albicans
from other
Candida
species are critical for the timely application of appropriate antimicrobial therapy, improved patient outcomes, and pharmaceutical cost savings. In this work, two 28S rRNA-directed peptide nucleic acid-fluorescence
in situ
hybridization (PNA-FISH) probes, P-Ca726 (targeting a novel region of the ribosome) and P-CalB2208 (targeting a previously reported region), were evaluated. Hybridization conditions were optimized by using both fluorescence microscopy (FM) and flow cytometry (FCM), and probes were screened for specificity and discriminative ability against a panel of
C. albicans
and various nontarget
Candida
spp. The performance of these PNA probes was compared quantitatively against that of DNA probes or DNA probe/helper combinations directed against the same target regions. Ratiometric analyses of FCM results indicated that both the hybridization quality and yield of the PNA probes were higher than those of the DNA probes. In FCM-based comparisons of the PNA probes, P-Ca726 was found to be highly specific, showing 2.5- to 5.5-fold-higher discriminatory power for
C. albicans
than P-CalB2208. The use of formamide further improved the performance of the new probe. Our results reinforce the significant practical and diagnostic advantages of PNA probes over their DNA counterparts for FISH and indicate that P-Ca726 may be used advantageously for the rapid and specific identification of
C. albicans
in clinical and related applications, especially when combined with FCM.
Evaluation of the Cobas TaqMan MTB Test for the Detection of Mycobacterium tuberculosis Complex According to Acid-Fast-Bacillus Smear Grades in Respiratory Specimens
Authors: Huh Hee Jae , Koh Won-Jung , Song Dong Joon , Ki Chang-Seok , Lee Nam Yong 
Published: November 2014

ABSTRACT
We evaluated the performance of the Cobas TaqMan MTB test (Roche Diagnostics, Basel, Switzerland), stratified by acid-fast bacilli (AFB) smear grades. The sensitivity of this test in smear-positive specimens was >95% in all grades, while that in trace and negative specimens was 85.3% and 34.4%, respectively.
Pott's Disease? AIDS-Associated Mycobacterium heckeshornense Spinal Osteomyelitis and Diskitis
Authors: Carpenter Robert J. , Graf Paul C. F. 
Published: November 2014

ABSTRACT

Acid-fast bacillus (AFB) spinal osteomyelitis in a patient with AIDS is often presumed to be caused by reactivated
Mycobacterium tuberculosis
. However, other AFB pathogens can mimic
M. tuberculosis
and, to ensure appropriate and adequate therapy, should be considered by clinicians. We present a case of aggressive spinal osteomyelitis caused by
Mycobacterium heckeshornense
in an AIDS patient; a review of the literature is also included.
A Case of IMP-4-, OXA-421-, OXA-96-, and CARB-2-Producing Acinetobacterpittii Sequence Type 119 in Australia
Authors: Kamolvit Witchuda , Derrington Petra , Paterson David L. , Sidjabat Hanna E. 
Published: November 2014

ABSTRACT

An IMP-4-producing
Acinetobacter
pittii
strain coproducing oxacillinases was isolated from a leg wound of a 67-year-old female patient. Identification to the species level by
rpoB
and
gyrB
sequencing and multiplex-PCR-based analysis revealed that the isolate was
A. pittii
. Whole-genome sequencing of this
A. pittii
isolate determined the presence of
bla
OXA-96
,
bla
CARB-2
, and a novel
bla
OXA-421
gene. The position of this novel
bla
OXA-421
gene was similar to that of
bla
OXA-51
in
A. baumannii
, downstream of the phosphinothricin
N
-acetyltransferase gene and upstream of
fxsA
in the chromosome. This
A. pittii
isolate was found to belong to sequence type 119 (ST119). Here, we report the first isolation of IMP-4-producing
A. pittii
ST119 with a novel
bla
OXA-421
gene from a patient in Australia and characterize its draft genome.
Canine Distemper Virus Antigen Detection in External Epithelia of Recently Vaccinated, Sick Dogs by Fluorescence Microscopy Is a Valuable Prognostic Indicator
Authors: Kapil Sanjay , Neel Tina 
Published: November 2014

ABSTRACT
Currently, there are no reliable predictors of the clinical outcomes of domesticated dogs that have been recently vaccinated against canine distemper virus (CDV) and develop respiratory disease. In this study, vaccinated dogs from Oklahoma City that were showing clinical signs of respiratory disease were evaluated for CDV antigen using a direct fluorescent antibody test (FAT). Clinical outcomes after standard symptomatic therapy for respiratory disease were recorded, and a statistical analysis of the results was performed. We present our study showing that CDV FAT results were predictive of clinical recovery (prognostic indicator, prospects of clinical recovery) among vaccinated dogs showing clinical signs of respiratory disease. Negative CDV FAT results equated to 80% chances of recovery after symptomatic therapy, compared to 55% chances of recovery when the CDV FAT results were positive. Based on the results of this study, we show that veterinarians can make better informed decisions about the clinical outcomes of suspected CDV cases, with 2-h turnaround times, by using the CDV FAT. Thus, antemortem examination with the CDV FAT on external epithelia of recently vaccinated, sick dogs is a clinically useful diagnostic test and valuable prognostic indicator for veterinarians. Application of the CDV FAT to these samples avoids unnecessary euthanasia of dogs with suspected CDV.