bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced purinergic signaling in the paraventricular hypothalamus induces hyperphagic obesity and insulin resistance
Authors: Liu Yu , Zhang Haiyang , Zhang Qian , Najam Syeda Sadia , Liang Xian , Sirakawin Chaweewan , Lin Dongfa , Huang Shangyuan , Bondarenko Anastasiia , Konopka Witold , Zhang Yi , Zhang Zhi , Wu Shihao , Jing Miao , Vinnikov Ilya A. 
Published: July 2024

AbstractBody energy homeostasis is tightly regulated by hypothalamic neural circuits. However, their remodeling upon metabolic stress remains incompletely characterized, translating to unprecedented challenges to develop safe medications against the surge of metabolic diseases. Oxytocin (OXT) neurons in the paraventricular nucleus of the hypothalamus (PVH) are one of the key appetite-suppressing effectors within the melanocortin system. In this work, we report that metabolic stress in mice evokes spatiotemporally selective ATP release (Inflares) from PVH astrocytes, accompanied with the expression of hematopoietic lineage-specific ADP/ATP receptor P2Y12on OXT (PVHOXT) neurons. Importantly, “ectopic” emergence of P2Y12on OXT neurons of patients with diabetes mellitus suggests an evolutionary conserved purinergic response to metabolic stress. Strikingly, increased purinergic signaling leads to impaired responsiveness of PVHOXTneurons accompanied with hyperphagic obesity and insulin resistance in mice. Moreover, nasal administration of clinically approved doses of P2Y12inhibitors counteracts diet-induced obesity and insulin resistance in mice and spontaneous weight gain in monkeys, paving the way for application of these compounds in patients with metabolic disorders.
Single-cell transcriptomics highlights sexual cues among reproductive life stages of uncultivated Acantharia (Radiolaria)
Authors: Rizos Iris , Romac Sarah , Juery Caroline , Berthelier Charlotte , Decelle Johan , Bernardes Juliana , Corre Erwan , Bittner Lucie , Not Fabrice 
Published: July 2024

AbstractAs an innate property of life, the ability to reproduce is a key process for the perpetuation of organisms. Along the evolution of protist reproductive strategies, the molecular machinery of sexual recombination is estimated to have been inherited from the last eukaryotic common ancestor (LECA). Nevertheless, unraveling the sexual cycles of extant free-living protist lineages remains challenging, given the enigmatic roles of many uncultivated life stages. Among the uncultivated planktonic group of Acantharia (Radiolaria), a hypothetical sexual cycle has been proposed since the late 19th century, including the existence of a gamete-like life stage of undetermined ploidy, referred to as swarmers. In order to investigate the sexual nature of acantharian reproductive stages, we conducted single-cell transcriptomic analysis across various acantharian life stages. Our results show distinct functional profiles for reproductive and vegetative life stages, while revealing the expression of the reference eukaryotic genes involved in gamete fusion, HAP2/GCS1 and GEX1-KAR5, in swarmers and pre-swarmer stages. Annotation of differentially expressed life stage-specific genes, also highlights putative meiosis-related functions among swarmers, while suggesting the existence of a potential swarmer/vegetative intermediate stage expressing putative growth-related genes. This original life stage-specific genetic data is coherent with morphological evidence supporting the existence of an acantharian sexual cycle, with swarmers acting as gametes. Moreover, it paves the way for a deeper understanding of radiolarian cell biology and ecology at a single-cell scale.HighlightsAcantharia demonstrate both morphological and genetic evidence of a sexual cycleAcantharian reproductive stages are enriched in functions related to cell divisionNuclear fusion gene family GEX1-KAR5 is up-regulated in putative acantharian gametesMost expressed genes specific to acantharian reproductive stages are unassignedReproduction-specific unassigned genes include putative sex-related functions
Exploring glioma heterogeneity through omics networks: from gene network discovery to causal insights and patient stratification
Authors: Kastendiek Nina , Coletti Roberta , Gross Thilo , Lopes Marta B. 
Published: July 2024

AbstractGliomas are primary malignant brain tumors with a typically poor prognosis, exhibiting significant heterogeneity across different cancer types. Each glioma type possesses distinct molecular characteristics determining patient prognosis and therapeutic options. This study aims to explore the molecular complexity of gliomas at the transcriptome level, employing a comprehensive approach grounded in network discovery. The graphical lasso method was used to estimate a gene co-expression network for each glioma type from a transcriptomics dataset. Causality was subsequently inferred from correlation networks by estimating the Jacobian matrix. The networks were then analyzed for gene importance using centrality measures and modularity detection, leading to the selection of genes that might play an important role in the disease. Spectral clustering based on patient similarity networks was applied to stratify patients into groups with similar molecular characteristics and to assess whether the resulting clusters align with the diagnosed glioma type. The results presented highlight the ability of the proposed methodology to uncover relevant genes associated with glioma intertumoral heterogeneity. Further investigation might encompass biological validation of the putative biomarkers disclosed.
Protease shaving ofMycobacterium tuberculosisfacilitates vaccine antigen discovery and delivery of novel cargoes to the Mtb surface
Authors: Lepe Bianca A. , Zheng Christine R. , Leddy Owen K. , Allsup Benjamin L. , Solomon Sydney L. , Bryson Bryan D. 
Published: July 2024

AbstractTuberculosis (TB), caused byMycobacterium tuberculosis(Mtb), is the leading cause of infectious disease death and lacks a vaccine capable of protecting adults from pulmonary TB. Studies have shown that Mtb uses a variety of mechanisms to evade host immunity. Secreted Mtb proteins such as Type VII secretion system substrates have been characterized for their ability to modulate anti-Mtb immunity; however, studies of other pathogens such asSalmonellaTyphi andStaphylococcus aureushave revealed that outer membrane proteins can also interact with the innate and adaptive immune system. The Mtb outer membrane proteome has received relatively less attention due to limited techniques available to interrogate this compartment. We filled this gap by deploying protease shaving and quantitative mass spectrometry to identify Mtb outer membrane proteins which serve as nodes in the Mtb-host interaction network. These analyses revealed several novel Mtb proteins on the Mtb surface largely derived from the PE/PPE class of Mtb proteins, including PPE18, a component of a leading Mtb vaccine candidate. We next exploited the localization of PPE18 to decorate the Mtb surface with heterologous proteins and deliver these surface-engineered Mtb to the phagosome. Together, these studies reveal potential novel targets for new Mtb vaccines as well as facilitate new approaches to study difficult to study cellular compartments during infection.
Progerin Can Induce DNA Damage in the Absence of Global Changes in Replication or Cell Proliferation
Authors: Joudeh Liza A. , Schuck P. Logan , Van Nina M. , DiCintio Alannah J. , Stewart Jason A. , Waldman Alan S. 
Published: July 2024

ABSTRACTHutchinson-Gilford Progeria Syndrome (HGPS) is a rare genetic condition characterized by features of accelerated aging, and individuals with HGPS seldom live beyond their mid-teens. The syndrome is commonly caused by a point mutation in the LMNA gene which codes for lamin A and its splice variant lamin C, components of the nuclear lamina. The mutation causing HGPS leads to production of a truncated, farnesylated form of lamin A referred to as “progerin.” Progerin is also expressed at low levels in healthy individuals and appears to play a role in normal aging. HGPS is associated with an accumulation of genomic DNA double-strand breaks (DSBs) and alterations in the nature of DSB repair. The source of DSBs in HGPS is often attributed to stalling and subsequent collapse of replication forks in conjunction with faulty recruitment of repair factors to damage sites. In this work, we used a model system involving immortalized human cell lines to investigate progerin-induced genomic damage. Using an immunofluorescence approach to visualize phosphorylated histone H2AX foci which mark sites of genomic damage, we report that cells engineered to express progerin displayed a significant elevation of endogenous damage in the absence of any change in the cell cycle profile or doubling time of cells. Genomic damage was enhanced and persistent in progerin-expressing cells treated with hydroxyurea. Overexpression of wild-type lamin A did not elicit the outcomes associated with progerin expression. Our results show that DNA damage caused by progerin can occur independently from global changes in replication or cell proliferation.
Optimizing Multi-Environment Trials in The US Rice Belt via Smart-Climate-Soil Prediction Based-Models and Economic Importance
Authors: Prado Melina , Famoso Adam , Guidry Kurt , Fritsche-Neto Roberto 
Published: July 2024

AbstractRice breeding programs globally have worked to release increasingly productive and climate-smart cultivars, but the genetic gains have been limited for some reasons. One is the capacity for field phenotyping, which presents elevated costs and an unclear approach to defining the number and allocation of multi-environmental trials (MET). To address this challenge, we used soil information and ten years of historical weather data from the USA rice belt, which was translated into rice response based on the rice cardinal temperatures and crop stages. Next, we eliminated those highly correlated Environmental Covariates (ECs) (>0.95) and applied a supervised algorithm for feature selection using two years of data (2021-22) and 25 genotypes evaluated for grain yield in 18 representative locations in the Southern USA. To test the trials’ optimization, we performed the joint analysis using prediction-based models in four different scenarios: I) considering trials as non-related, ii) including the environmental relationship matrix calculated from ECs, iii) within clusters; iv) sampling one location per cluster. Finally, we weigh the trial’s allocation considering the counties’ economic importance and the environmental group to which they belong. Our findings show that eight ECs explained 58% of grain yield variation across sites and 53% of the observed GxE. Moreover, it is possible to reduce 28% the number of locations without significant loss in accuracy. Furthermore, the US Rice belt comprises four clusters, with economic importance varying from 13 to 45%. These results will help us better allocate trials in advance and reduce costs without penalizing accuracy.
Synthesis and evaluation of Schiff base as corrosion inhibitor for carbon steel in 1 M HCl solution
Authors: Jafari Hojat , Ameri Elham 
Published: July 2024

Purpose
The main purpose of the present study is to introduce new Schiff bases as corrosion inhibitor for carbon steel in 1 M HCl. The inhibitory activity of Schiff base was also assessed.

Design/methodology/approach
2,2′-((1Z,1′Z)-((2,2-dimethylpropane-1,3-diyl)bis(azanylylidene))bis(methanylylidene))diphenol was synthesized and it’s performance as an inhibitor was then investigated in 1 M HCl. The inhibition of this compound was studied and evaluated by the chemical methods of electrochemical impedance spectroscopy, electrochemical potential dynamic polarization and Atomic Force microscopy (AFM) method. The thermodynamics parameters were investigated for corrosion of carbon steel in both the absence and presence of Schiff base.

Findings
The results of the tests showed that this compound has a good performance as an inhibitor and the percentage of inhibition on steel corrosion will increase with increasing concentration and it will reach 70% in the presence of 2 × 10−3 M of this inhibitor. Polarization tests indicated that this compound will act as a mixed inhibitor. Nyquist curves showed that the addition of this substance to the solution increased the charge transfer resistance and decreased the capacity of the double layer. The absorption of the new Schiff base on steel follows Langmuir adsorption isotherm, and the amount of free energy of adsorption indicates the spontaneous adsorption of this inhibitor. Using AFM investigations, the results of electrochemical methods were confirmed.

Originality/value
Incorporation of a new Schiff base into 1 M HCl is a promising approach for protecting the carbon steel against corrosive solution.
Assessing competitiveness through intellectual capital research: a systematic literature review and agenda for future research
Authors: Abdallah Amr S. , Amin Hala M.G. , Abdelghany Mohammed , Elamer Ahmed A. 
Published: July 2024

Purpose
The purpose of this study is to undertake a systematic literature review (SLR) on intellectual capital disclosure (ICD), focusing on its role in fostering competitive advantage.

Design/methodology/approach
Following the SLR process, the study identified 84 papers published in high-ranking journals over a 19-year span, providing insights into descriptive outcomes, research limitations and future research directions.

Findings
The results show that ICD research peaked in 2022, with the Journal of Intellectual Capital leading with the highest number of ICD publications. Resource-based theory was found to be the most applied theoretical framework, with developed country-specific research receiving the most attention. The use of small sample size, a lack of longitudinal studies, reliance on a single source of data, unsuitability of control variables and a lack of comparative studies with firms operating in developing countries are the main limitations that have been noted.

Research limitations/implications
This study faces constraints, primarily stemming from the selective keyword utilization and exclusive Scopus database reliance. It omits non-English papers, conference proceedings and books, potentially overlooking relevant insights.

Practical implications
The findings offer valuable insight for researchers, emphasizing the need for research on intellectual capital (IC) across diverse industries. Furthermore, our findings urge regulators to mandate global IC reporting to mitigate information asymmetry, while also prompting managers to enhance IC-related practices and reporting for more stakeholders’ trust.

Originality/value
This study provides a comprehensive overview of over two decades of ICD literature, synthesizing previous studies, identifying gaps and outlining potential directions for scholars and industry professionals in the context of competitiveness.
Who gets the best of you? Coaching to develop emotional intelligence in and out of work
Authors: Wittmer Jenell Lynn-Senter 
Published: July 2024

Purpose
The purpose of the current study is to determine the extent to which individuals who are being coached to develop emotional intelligence utilize these competencies in their work versus their personal lives.

Design/methodology/approach
Individuals, as part of a leadership development program or stand-alone executive coaching, were asked to self-report the extent to which they utilize emotional intelligence competencies in both their work and personal lives. These individuals were questioned at two time periods (Time 1N = 172; Time 2 N = 151), six months apart.

Findings
The findings overwhelmingly support that individuals report using emotional intelligence competencies more at work than in their personal lives at both times periods. There was, however, a substantial increase in those reporting utilizing emotional intelligence competencies in their personal life after participating in executive coaching.

Originality/value
Much research has examined the positive impacts that emotional intelligence has in the workplace. However, less research has examined its impact on individuals’ personal lives. Little to no research has examined how organizational interventions can help increase the positive impact of emotional intelligence outside of the workplace.
Hybrid working – Benefits and challenges for productivity and performance
Authors: Williams Simon , Shaw Nicky 
Published: July 2024

PurposeWe explore the benefits and challenges for organisations using hybrid working practices post-pandemic. We focus upon the non-profit English social housing sector; however, this research is relevant to any organisation adopting hybrid working practices. The implications for productivity and performance management of hybrid practices are currently not well understood.Design/methodology/approachWithin this Reflective Practice work, we apply a dual-theoretic lens to a new Ways of Working tool and adopt a mixed methods approach. First scoping interviews were conducted with senior managers possessing strategic knowledge of their organisations’ approaches to hybrid working. Secondly, a large-scale survey was administered to evaluate employees’ perceptions of hybrid working, including their concerns for future impacts.FindingsOur data identify several benefits and challenges associated with hybrid working. Reclaimed commuting time was deemed a positive benefit, alongside greater personal time, work-life balance and (for the majority) less-interrupted workspace. Challenges were identified regarding the transparency of staff reward and recognition, the potential for masked burnout and purposeful team communication.Research limitations/implicationsDue to the unique context of the pandemic and widespread, but sudden adoption of home- and hybrid working, this study can be taken as a snapshot in time as organisations recalibrate the consequences of new ways of working.Originality/valueDespite hybrid working having been possible for decades, many articles describe typical benefits (e.g. less commuting time) and challenges (e.g. organisational culture) without fully understanding productivity and performance implications. To explore this, we extend Palvalin et al.’s (2015) Tool by establishing a theoretical foundation through the conservation of resources theory and practice theory.
Citizen satisfaction with arm's length bodies in local government: expert insights on policy and practice
Authors: Angius Virginia , van Thiel Sandra 
Published: July 2024

PurposeThe purpose of this study is to investigate the application of citizen satisfaction (CS) as a tool for measuring performance by arm's length bodies (ALBs) in local governments across eleven countries, addressing central questions about its functionality and influencing factors.Design/methodology/approachWe employ a hybrid inductive-deductive explorative study design, characterized by an iterative interpretative approach guided by sensitizing concepts. Expert interviews are analyzed through template analysis and cover eleven countries where ALBs are present due to decentralization and outsourcing.FindingsOur study uncovers limited real-world applications of CS in evaluating public service delivery by ALBs. Expert interviews reveal insights into factors potentially influencing CS use, such as the level of autonomy of local governments, state traditions and austerity measures. A research agenda is formulated for further investigation.Originality/valueContributing to the CS debate, our study focuses on its application in evaluating public service delivery by ALBs. The explorative design, expert opinions and theoretical foundations enhance understanding, offering insights into factors potentially shaping CS use in this context. The study proposes a research agenda, propelling further investigations into this underexplored, yet emerging, area.
The role of key actors in relation to discourse in a microblogging hashtag stream
Authors: Sharma Priya , Sandoval-Llanos Jose , Foster Daniel , Miller Foster Melanie 
Published: July 2024

Purpose
This study aims to examine the role of key network actors in relation to the discourse structure of a microblogging hashtag stream within a global agricultural educators’ conference over two years. Prior work in online networks suggests that participation is dominated by highly active members, and in this study, the authors focus on examining what types of discourse are shared and reshared by key actors.

Design/methodology/approach
The authors used a combination of social network analyses and qualitative discourse coding to examine approximately 1,390 posts associated with the conference hashtag over two consecutive years.

Findings
The study analyses uncovered a set of common key participants over both years and common types of discourse used by those key participants. Key participants took on roles of resharing messages and contributed to discourse by retweeting posts that highlighted participants’ thoughts and feelings related to the conference and the discipline.

Research limitations/implications
This research has implications for encouraging diverse participants and diverse discourses related to key community goals. Design suggestions include identifying and inviting key actors as collaborators to reshare discourse that clearly aligns with community goals and using smaller hashtag spaces to encourage broader participation.

Originality/value
Prior work on microblogging has highlighted either the types of discourse and information sharing or the structures of the network interactions within conference hashtag streams. This study builds on this prior work and combines discourse and structure to understand the ways in which key network figures reshare discourse within the community, a facet that has been underreported in the literature.
The relationship between feminist collective action and social media engagement
Authors: Buran İrem , Sabah Şenay , Koçak Akin 
Published: July 2024

Purpose
This study aims to investigate the impact of online collective feminist actions on social media participation and the perceived value of social media as part of the social identity model of collective action (SIMCA).

Design/methodology/approach
A mixed methodology is used. Within the SIMCA model in the context of feminist collective actions, social identity, group-efficacy and fear predicted the intention of online collective action participation in the first part (quantitative) of the study. Contrary to predictions, the influence of anger on the intention to participate in collective action was negative. In-depth interviews are conducted in the study’s second (qualitative) part to investigate why individuals do not participate in collective actions despite their anger at violence against women.

Findings
The concept of online feminist collective action, from the perspective of the SIMCA model, deals with violence against women in the context of social marketing, revealing the importance of online collective actions as an antecedent of social media participation and the perceived value of social media. The possible causes of the negative impact of anger, which is an important emotion within the framework of the SIMCA model, on online feminist collective action were determined, and a contribution was made to the social marketing literature in the context of women’s rights.

Originality/value
The study makes three major contributions to the literature. First, women’s rights are addressed in the context of online collective action, an issue that has received little attention in social marketing. Second, in the context of the social identity theory of collective action, online collective feminist action and its predecessors are addressed. Finally, the Turkish case is used to highlight the probable causes of anger’s negative impact on collective action.