bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete genome sequence of bacteriophage P8625, the first lytic phage that infects Verrucomicrobia
Authors: Choi Ahyoung , Kang Ilnam , Yang Seung-Jo , Cho Jang-Cheon 
Published: November 2015

AbstractBacteriophage P8625 is a lytic bacteriophage that infects the verrucomicrobial strain IMCC8625, a marine bacterium affiliated with Verrucomicrobia subdivision 4. Both the bacteriophage and the host bacterial strain were isolated from surface seawater samples collected off the east coast of Korea. The phage particle has an icosahedral capsid with a diameter of ~47 nm and a long tail of ~75 nm in length, showing the distinctive morphology of the Siphoviridae family. The complete genome sequence of phage P8625 is 32,894 bp long with 51.0 % G + C content. This is the first report of the complete genome sequence of a lytic phage that infects the Verrucomicrobia, for which the name “verrucophage” is proposed.
EEG correlates of social interaction at distance
Authors: Giroldini William , Pederzoli Luciano , Bilucaglia Marco , Caini Patrizio , Ferrini Alessandro , Melloni Simone , Prati Elena , Tressoldi Patrizio E. 
Published: November 2015

This study investigated EEG correlates of social interaction at distance between twenty-five pairs of participants who were not connected by any traditional channels of communication.Each session involved the application of 128 stimulations separated by intervals of random duration ranging from 4 to 6 seconds. One of the pair received a one-second stimulation from a light signal produced by an arrangement of red LEDs, and a simultaneous 500 Hz sinusoidal audio signal of the same length. The other member of the pair sat in an isolated sound-proof room, such that any sensory interaction between the pair was impossible.An analysis of the Event-Related Potentials associated with sensory stimulation using traditional averaging methods showed a distinct peak at approximately 300 ms, but only in the EEG activity of subjects who were directly stimulated. However, when a new algorithm was applied to the EEG activity based on the correlation between signals from all active electrodes, a weak but robust response was also detected in the EEG activity of the passive member of the pair, particularly within 9 – 10 Hz in the Alpha range. Using the Bootstrap method and the Monte Carlo emulation, this signal was found to be statistically significant.
‘I can't walk away’: understanding the complexities in embedding a new nurse role
Authors: Randall Sue , Furze Gill , Thunhurst Colin 
Published: November 2015

Background: The community matron (CM) role was introduced in England to provide a single point of access to patients living with comorbid long-term conditions who had, or were at risk of, frequent emergency hospitalisation. Aim: This study explored the factors that affected embedding of the CM role. The qualitative study was undertaken in community health services in two cities and a rural area in central England. Method: Participants comprised health professionals (n=30) including CMs, community service managers, and service commissioners; patients (n=10); and their family carers (n=5). Results: Findings reveal that participants were largely positive about the role. However, difficulties with role setup had led to numerous changes in service delivery, which affected how the role has embedded. Conclusion: Many aspects of the CM role are invisible to other health professionals. Invisibility of community nursing, rather than autonomy of the CM role, seems to be a key factor in the challenges of embedding the role.
Is frenotomy effective in improving breastfeeding in newborn babies with tongue-tie? A literature review
Authors: Burrows Sally , Lanlehin Rosemary 
Published: November 2015

Aim: This literature review explores whether frenotomy can result in more successful breastfeeding of newborn infants with ankyloglossia. Method: A systematic literature search was carried out. Nine pieces of research were chosen and appraised using two critical appraisal tools. Findings: Three main themes emerged: the mother's experiences of breastfeeding; duration of breastfeeding; and latch. The majority of studies found that frenotomy could improve latch and alleviate nipple pain. However, there is little information available about outcomes for babies with ankyloglossia who do not undergo the frenotomy procedure. Conclusion: Frenotomy is a procedure that can benefit breastfeeding mothers and babies, but the cultural and social background of families is not addressed in much of the research, nor is the risk of distress to the infant and parents.
Knowledge of pregnancy danger signs and associated factors among Malaysian mothers
Authors: Teng See Poh , Zuo Tey Chiao , Jummaat Fauziah Binti , Keng Soon Lean 
Published: November 2015

Background:Pregnancy complications are a main cause of maternal mortality. Studies suggest not all women regard pregnancy complications as abnormal, owing to a lack of knowledge about ‘danger signs’. Danger signs in pregnancy include vaginal bleeding, severe headache or blurred vision, severe abdominal pain, swollen hands and face, reduced fetal movement, weakness and difficulty in breathing.Aims:To assess the level and associated factors of knowledge about pregnancy danger signs among antenatal women in Malaysia.Methods:A cross-sectional design using a modified questionnaire was employed with 178 antenatal women.Results:More than half of the antenatal women were lacking in knowledge of pregnancy danger signs. A significant association was found between the age, level of education, working status and monthly household income of antenatal women and their level of knowledge.Conclusion:The study revealed the knowledge level of these antenatal women about pregnancy danger signs was inadequate. Therefore, it is necessary for maternal health services to empower antenatal women to understand the danger signs of pregnancy complications.
Investigating the pressure-reducing effect of wound dressings
Authors: Matsuzaki K. , Kishi K. 
Published: November 2015

Objective: Currently, polyurethane foam dressings are commercially available from many manufacturers. However, the pressure-reducing effect is expected to differ by the formulation and combination of the main and secondary ingredients and by manufacturing method. In this study, we investigated the effects of pressure reduction using dressing materials with various structural characteristics, including polyurethane foam dressings based on the engineering point of view, focusing on the dry state. Method: Pressure was measured in a model that simulated compression on the sacral region in a decubitus position. Pressure was measured for different dressings: ten products, consisting of five types of material (polyurethane foam, hydropolymeric, Hydrofiber, hydrocolloid, and low-adherent absorbent). Results: All dressings used in this study showed significantly reduced pressure. ALLEVYN Non-Adhesive had the lowest pressure at 35.833 ± 1.155 mmHg, and DuoDERM Extra Thin CGF had the highest pressure at 66.867 ± 1.060 mmHg. The pressure of the control was 74.667 ± 1.405 mmHg. The other dressings were: ALLEVYN Adhesive: 44.233 ± 0.777 mmHg; ALLEVYN Gentle Border: 46.967 ± 1.537mmHg; Mepilex Border: 53.867 ± 0.231 mmHg; Biatain Silicone: 56.000 ± 0.520 mmHg; TIELLE: 57.267 ± 3.403 mmHg;Versiva XC: 65.900 ± 0.800 mmHg; DuoDERM CGF: 57.267 ± 1.007 mmHg; and Melolin: 53.433 ± 1.973 mmHg. Conclusion: The pressure-reducing effect of dressing differs not only by material type but also by product. That is, the pressure-reducing effect can differ even if the dressings are of the same material type, such as polyurethane foam. Our study investigated only the effect of materials and structural characteristics on the cushion of dressings in the dry state. Therefore, further investigation is needed to confirm the effect of pressure reduction by dressing to meet the conditions in the clinic.
Dehydrated human amnion/chorion membrane treatment of venous leg ulcers: correlation between 4-week and 24-week outcomes
Authors: Serena T.E. , Yaakov R. , DiMarco D. , Le L. , Taffe E. , Donaldson M. , Miller M. 
Published: November 2015

Objective: To evaluate correct correlation between an intermediate rate of wound reduction (40% wound area reduction after 4-weeks treatment) and complete healing at 24 weeks in patients with a venous leg ulcer (VLU). Method: A retrospective follow-up study of patients completing a multicenter randomised controlled trial (RCT) comparing the number of patients achieving at least 40% healing of their VLU within 4-weeks of treatment with either dehydrated human amnion/chorion membrane (dHACM) allograft or multilayer compression only was conducted. Outcomes assessed were rates of complete healing within 24 weeks of enrolment and days to healing. Data were divided into two groups based on status at RCT completion (healed at least 40% yes or no). Correct correlation with status at 4 weeks and complete healing within 24 weeks was determined. Clinical characteristics were also compared for patients with and without correct correlation between 4-week and 24-week status. Results: We identified 55 patients at 5 study sites. Some 47 without complete healing during the initial study were eligible. As three patients were lost to follow-up we evaluated 44 records. Of these, 20 (45.4%) had reduced wound size of ≥40% and 24 (55%) had <40% reduction during the initial study. Complete healing occurred in 16/20 (80%) of the ≥40% group at a mean of 46 days, p=0.0027 and 8/24 (33.3%) of the <40% group at a mean of 103.6 days, p=0.0023. Overall, correct correlation of status at 4 weeks and ultimate healing status of VLU occurred in 32/44 patients (73%). Conclusion: These results confirm that the intermediate outcome used in our initial study is a viable predictor of ultimate VLU healing.
Effective Use of Mass Spectrometry in the Clinical Laboratory
Authors: Jannetto Paul J , Fitzgerald Robert L 
Published: November 2015

Abstract

BACKGROUND
Historically the success of mass spectrometry in the clinical laboratory has focused on drugs of abuse confirmations, newborn screening, and steroid analysis. Clinical applications of mass spectrometry continue to expand, and mass spectrometry is now being used in almost all areas of laboratory medicine.

CONTENT
A brief background of the evolution of mass spectrometry in the clinical laboratory is provided with a discussion of future applications. Prominent examples of mass spectrometry are covered to illustrate how it has improved the practice of medicine and enabled physicians to provide better patient care. With increasing economic pressures and decreasing laboratory test reimbursement, mass spectrometry testing has been shown to provide cost-effective solutions. In addition to pointing out the numerous benefits, the challenges of implementing mass spectrometry in the clinical laboratory are also covered.

SUMMARY
Mass spectrometry continues to play a prominent role in the field of laboratory medicine. The advancement of this technology along with the development of new applications will only accelerate the incorporation of mass spectrometry into more areas of medicine.
Planejamento da logística de suprimento de plataformas Offshore por meio de um modelo matemático 2L-CVRP com frota heterogênea e equilíbrio náutico
Authors: Arpini Bianca Passos , Rosa Rodrigo Alvarenga 
Published: November 2015

<p>No Brasil a exploração de petróleo ocorre, sobretudo, no mar onde são implantadas plataformas <em>offshore</em> que estão distantes da costa. Logo, é fundamental planejar a logística de suprimento por meio de navios. Este artigo propõe um modelo matemático de Programação Linear Inteira Mista denominado <em>Weight Balance Two-Dimensional Loading Heterogeneous Fleet Vehicle Routing Problem</em> (WB2L-HFVRP) aplicado ao planejamento da logística de suprimento de plataformas <em>offshore</em> visando a criação de rotas que considerem o equilíbrio náutico e a melhor arrumação das cargas no convés. Este modelo se diferencia por considerar frota heterogênea e utilizar uma função objetivo que visa minimizar o número de navios, a distância navegada e a diferença entre os pesos distribuídos entre os bordos do navio para manter o equilíbrio náutico. Testou-se o modelo em instâncias baseadas em dados reais da Petrobras no CPLEX 12.6 e alcançou-se uma redução de até 25% em relação à distância real navegada.</p>
Lawful Solutions For Violation On Women In Sports
Authors: Sakáčová Zuzana 
Published: November 2015

Summary
This contribution deals with the legal aspects and acts of violence against a group of women in sport. Discussions about violence in society and thus on violence in sport are often associated with people's thoughts about the moral state of society as a whole. Violence against women is one of the most widespread violations of human dignity. It represents a violation of human rights, namely the right to life, the right to safety, the right to dignity, the right to physical and mental integrity. It is desirable to raise awareness about the prevention of sexual violence at all levels. We have to focus on preventive precautions to support projects to fight violence and intolerance in sport, to develop codes of conduct that determine the relationship between athletes, relations between men and women, as well as relations between coaches and officials, relations between parents and coaches. The Slovak Republic is only at the beginning of the way of creating the relationship between law and sport. Therefore, it is necessary to increase the legal awareness of sport as a tool for regulation in sport activities.
Numerical Modelling Of Humid Air Flow Around A Porous Body
Authors: Bohojło-Wiśniewska Aneta 
Published: November 2015

Summary
This paper presents an example of humid air flow around a single head of Chinese cabbage under conditions of complex heat transfer. This kind of numerical simulation allows us to create a heat and humidity transfer model between the Chinese cabbage and the flowing humid air. The calculations utilize the heat transfer model in porous medium, which includes the temperature difference between the solid (vegetable tissue) and fluid (air) phases of the porous medium. Modelling and calculations were performed in ANSYS Fluent 14.5 software.
Parametric Integral Equations Systems Method In Solving Unsteady Heat Transfer Problems For Laser Heated Materials
Authors: Sawicki Dominik , Zieniuk Eugeniusz 
Published: November 2015

Abstract
One of the most popular applications of high power lasers is heating of the surface layer of a material, in order to change its properties. Numerical methods allow an easy and fast way to simulate the heating process inside of the material. The most popular numerical methods FEM and BEM, used to simulate this kind of processes have one fundamental defect, which is the necessity of discretization of the boundary or the domain. An alternative to avoid the mentioned problem are parametric integral equations systems (PIES), which do not require classical discretization of the boundary and the domain while being numerically solved. PIES method was previously used with success to solve steady-state problems, as well as transient heat transfer problems. The purpose of this paper is to test the efficacy of the PIES method with time discretization in solving problem of laser heating of a material, with different pulse shape approximation functions.
Associated Behavioral Risks in a Group of Women During Pregnancy from Mures District
Authors: Florina Ruţa , Călin Avram , Septimiu Voidăzan , Victoria Rus , Mihai Georgescu , Zoltan Abram , Monica Tarcea 
Published: November 2015

Abstract

Background. It is well-known that the exposure during pregnancy to lifestyle risk factors can affect thehealth both for the mother and the fetus. There are many risk factors for pregnancy, including: smoking, alcohol consumption, sedentary pattern, irregular daily meal serving plan, lack of knowledge regarding health prevention. The main objective of our study was to assess the practices, knowledge and attitudes towards lifestyle risk behaviors during pregnancy in a group of women from Tirgu-Mures area.

Materials and methods. We used a cross-sectional study based on a questionnaire, assessingsmoking behavior and lifestyle patterns during pregnancy, on a group of 481 women from the urban area (58.84%), as well as from the rural area (41.16%) from Mures county.

Results. In the study group, 190 women (39.5%) used to smoke before pregnancy; among which 37.36% continued to smoke even after they found out they are pregnant. In the group of pregnant women that continued to smoke during pregnancy, we have studied other behavioral factors related to lifestyle, identifying the following: 5,73% have been consuming alcohol weekly, 56.33% reduced greatly their physical activity level, 3.63% have been consuming at least 3 cups of coffee a day. During pregnancy, 12.72% did not have a regular, daily meal serving plan, and 67.26% was a little or not at all concerned about their eating habits. Only 30.9% from the smoking pregnant women group have received information from the family doctor (GP) regarding the risk factors of smoking habits, while 38.18% did not receive any information.

Conclusions. Behavioral risk factors in pregnancy have to be followed closely by the HealthCare System in order to avoid complications for the mother and the child. The best way of accomplishing that is through an efficient community intervention.
Isolation, Characterization and Differentiation Potential of Chicken Spermatogonial Stem Cell Derived Embryoid Bodies
Authors: Nguyen Thanh Luan , Yoo Jae Gyu , Sharma Neelesh , Kim Sung Woo , Kang Yong Jun , Thi Hai Ha Pham , Jeong Dong Kee 
Published: November 2015

Abstract
Human, murine and monkey spermatogonial stem cells (SSCs) have the capability to undergo self-renewal and differentiation into different body cell types in vitro, which are expected to serve as a powerful tool and resource for the developmental biology and regenerative medicine. We have successfully isolated and characterized the chicken SSCs from 3-day-old chicken testicular cells. The pluripotency was using Periodic Acid-Schiff (PAS ) staining or alkaline phosphatase staining, and antibodies to stage-specific embryonic antigens. In suspension culture conditions SSCs formed embryoid bodies (EBs) like embryonic stem (ES) cells. Subsequently EB differentiated into osteoblasts, adipocytes and most importantly into cardiomyocytes under induced differentiation conditions. The differentiation potential of EBs into cardiomyocyte-like cells was confirmed by using antibodies against sarcomeric α-actinin, cardiac troponin T and connexin 43. Cardiomyocytes-like cells were also confirmed by RT-PCR analysis for several cardiac cell genes like GATA-4, Nkx2-5, α-MHC, and ANF. We have successfully established an in vitro differentiation system for chicken SSCs into different body cells such as osteoblasts, adipocytes and cardiomyocytes. The most significant finding of this study is the differentiation potential of chicken SSCs into cardiomyocytes. Our findings may have implication in developmental biology and regenerative medicine by using chicken as the most potential animal model.
5. MMP-2, TIMP-2, TAZ and MEF2a Transcript Expression in Osteogenic and Adipogenic Differentiation of Porcine Mesenchymal Stem Cells
Authors: Opiela Jolanta , Lipiński Daniel , Romanek Joanna , Juzwa Wojciech , Bochenek Michał , Wilczek Piotr 
Published: November 2015

Abstract
Mesenchymal stem cell (MSC) differentiation is regulated intrinsically by transcription factors and extrinsically by the extracellular matrix. We tested whether matrix metalloproteinase-2 (MMP-2) and its inhibitor TIMP-2, MEF2a and TAZ transcription factors are involved in porcine MSC differentiation towards adipocytes and osteocytes. Flow cytometry and immunofluorescence were used to investigate the expression levels of multipotent cell surface markers CD73 and CD105. Real- time PCR was performed to detect the osteogenic- and adipogenic-specific markers, osteocalcin and aP2, respectively, and to estimate the MMP-2, TIMP-2, MEF2a and TAZ transcript expression levels in three groups of cell, i.e., undifferentiated MSCs, adipocytes (A) and osteocytes (O). We showed that at the transcript level, the differentiation of MSCs towards adipocyte fate may involve MMP-2, TIMP-2 and TAZ. We also show that the differentiation of MSCs toward osteocyte fate may involve TIMP-2, MEF2a and TAZ. Our research provides preliminary data on the possible role of the MMP-2, TIMP-2 and TAZ transcripts in adipogenic differentiation and of the TIMP-2, TAZ and MEF2a transcripts in the osteogenic differentiation of porcine MSCs. We report for the first time the possible involvement of MEF2a in the osteogenesis of porcine MSCs. Our work may provide additional evidence for the MMP-independent function of TIMP-2 during osteogenesis.
Influence of Protein Supply on Threonine Efficiency and Threonine Catabolism in Hepatic Mitochondria of Chicks and Rats
Authors: Lee Chul Won , Zhao Rongjie , Cho Il Je , Byun Sung Hui , Kim Young Woo , Kim Youn Sook , Kim Sang Chan , An Won G. 
Published: November 2015

Abstract
This research investigated the relationship between efficiency of threonine (Thr) utilization and Thr oxidation in hepatic mitochondria in chicks and rats fed with graded levels (5.5-33.0% CP for chicks, 6-24% CP for rats) of protein. Calculation of efficiency of Thr utilization was based on N-balance data and an exponential N-utilization model, and Thr dehydrogenase (TDG) activity was determined. According to the results, no significant effect on TDG activity was observed in the liver of chicks who received diets containing from 5.5 up to 16.5% CP. However, significantly elevated TDG activities were observed, despite limited supply of Thr in protein, with diets containing from 22.0 up to 33.0% CP. At the levels of CP content from 5.5 up to 27.5%, no significant change in efficiencies of Thr utilization was observed. However, a significant decrease in efficiency was observed with diets containing from 27.5 to 33.0% CP. In chicks, the relationship between oxidation of Thr and Thr efficiency was observed with graded CP levels. In addition, elevated TDG activities in rat liver were observed with diets containing from 6.0 to 12.0% CP. At the levels of CP content from 6.0 up to 24.0%, no significant effect on efficiency of Thr utilization was observed in rats. In addition, no relationship was observed between Thr oxidation and efficiency of Thr utilization with graded CP levels in rats. Taken together, reactions for TDG appear to be animal species-dependent.