bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vogt‐Koyanagi‐Harada's Syndrome Associated with Hashimoto's Thyroiditis
Authors: Chi Hyung‐In , Furue Masutaka , Ishibashi Yasumasa 
Published: April 2015

AbstractVogt‐Koyanagi‐Harada's syndrome has been known as an autoimmune disease which affects the melanocyte‐containing tissues. We report a case of Vogt‐Koyanagi‐Harada's syndrome associated with Hashimoto's thyroiditis. IgM‐class antibodies against nevus cells were detected in the patient's serum by indirect immunofluorescence test. These clinical and laboratory findings support the autoimmune theory of this rare syndrome.
Dermatomyositis with Multiple Skin Ulcers Showing Vasculitis and Membrano‐cystic Lesion
Authors: Yamamoto Toshiyuki , Ohkubo Hiroko , Katayama Ichiro , Nishioka Kiyoshi 
Published: April 2015

AbstractA 63‐year‐old woman with dermatomyositis (DM) developed multiple punched‐out ulcers of the skin. She had not only a past history of gastric cancer 10 years prior to the onset of DM, but also a familial history of cancer. A skin biopsy from one of the ulcers revealed both vasculitis and membrano‐cystic lesion in the same specimen. She died of interstitial pneumonitis one year after the onset of DM. Vasculitis and membrano‐cystic lesion may be an important marker of a poor prognosis in DM.
Pyoderma Gangrenosum on the Finger
Authors: Nihei Yasuko , Yamada Mina , Tsukimoto Atsumi , Kawashima Makoto 
Published: April 2015

AbstractA case of pyoderma gangrenosum on the dorsal aspect of the right second and third fingers of a 50‐year‐old woman is reported. The patient had been wounded at the above‐mentioned site by a cutter. This wound developed into a painful ulcer which enlarged rapidly. No bacterial infections were detected. Histologically, an inflammatory infiltrate of lymphocytes mixed with neutrophils and nucleodusts was seen throughout the entire dermis, but there was no evidence of vasculitis. Based on this data, the case was diagnosed as pyoderma gangrenosum. The patient responded to treatment with systemic corticosteroids. Pyoderma gangrenosum at this site of the fingers seems to be rare.
Interferon‐γ‐induced HLA‐DR, but not ICAM‐1, Expression of Human Keratinocytes Is Down‐regulated by Calmodulin Antagonist
Authors: Saitoh Atsushi , Osada Atsushi , Kitajima Yasuo , Furue Masutaka , Tamaki Kunihiko 
Published: April 2015

AbstractInterferon‐γ (IFN‐γ) has been shown to induce or enhance the expression of MHC class II and intercellular adhesion molecule‐1 (ICAM‐1) in a variety of human and murine cell types, including epidermal keratinocytes (KC). However, the expression of MHC class II and ICAM‐1 molecules induced by IFN‐γ is not necessarily coordinated. We investigated the inhibitory effects of the calmodulin antagonist, W‐7, and its chlorine deficient inactive analogue, W‐5, on the expression of MHC class II (HLA‐DR) and ICAM‐1 by human KC incubated with IFN‐γ. We found that the IFN‐γ‐induced expression of HLA‐DR was reproducibly and dose‐dependently inhibited by W‐7. However, the expression of ICAM‐1 was highly resistant to the inhibitory effects of W‐7. Neither HLA‐DR nor ICAM‐1 expression was affected by W‐5. These data suggest that the IFN‐γ‐induced HLA‐DR, but not ICAM‐1, expression is mediated, if not exclusively, by calmodulin in human KC.
Probable Mechanisms of Loss of Merkel Cells in Completely Depigmented Skin of Stable Vitiligo
Authors: Bose Sumit Kumar 
Published: April 2015

AbstractVitiligo is frequently associated with segmental involvement. It spares paralyzed limbs in transverse myelitis. There is spontaneous repigmentation of vitiligo lesional skin (VL) in diabetic neuropathy. Increased neuropeptide in VL and adjacent normal skin of vitiligo (ANS) along with thickened nerve fibers showing ultrastructural abnormalities all indicate a neural pathogenesis (1). Loss of Merkel cells has been observed in early vitiligo lesional skin (2). Recently, catecholamines have been found to play a major role in initiating the cascade of events leading to loss of melanocytes (1, 3, 4).To investigate the presence of Merkel cells in the completely depigmented skin of stable vitiligo (SV), a study was undertaken using TROMA 1, a monoclonal antibody specific for adult Merkel cells. No TROMA 1‐positive cells were observed in SV, whereas normal numbers of these cells were seen in ANS. This new finding further supports the hypothesis of neural involvement in vitiligo.
Pre‐Existing Cutaneous Changes in Basal Cell Carcinomas of the Lower Extremities
Authors: Betti Roberto , Inselvini Elena , Perotta Elena , Crosti Carlo 
Published: April 2015

AbstractBasal cell carcinomas (BCCs) of the lower extremities are not frequently reported. Some pre‐existing or predisposing conditions are thought to be implicated in their development. In this study, we investigated the possible relationships between pre‐existing cutaneous changes and the development of BCCs of the lower extremities, possible sex and age differences, and the histologic patterns involved. Among 1053 patients with BSSc, BCCs of the lower extremities were observed in 48 (4.5%): 25 men and 23 women. Lesions appeared on the thighs (16 cases), the lower legs (27 cases), or the feet (5 cases). Histologic patterns were nodular and micronodular (18 cases), superficial (27 cases), or infiltrative (3 cases). No differences were observed between sexes. The relative degree of elastosis was higher in the lower limbs of women than of men (2.25 vs 1.53).Pre‐existing cutaneous changes were observed in 7 cases. The frequency of these BCCs of the lower limbs and the higher degree of elastosis observed in the women seem to be related to sun exposure. The histologic pattern seems not to be affected by environmental factors but rather characteristic of body site. Pre‐existent cutaneous changes are not thought to be possible etiologic factors.
The Rash of Measles Is Caused by a Viral Infection in the Cells of the Skin: A Case Report
Authors: Makino Sagae , Yamaguchi Fumio , Sata Tetsutaro , Urushibata Osamu , Kurata Takeshi , Nishiwaki Munekazu 
Published: April 2015

AbstractMeasles skin rash was immunohistochemically examined in an effort to detect virus antigen in skin samples taken from a 15‐year‐old girl with measles. A sectioned specimen obtained by punch biopsy from a 2nd‐day skin lesion showed localized parakeratosis and acanthosis with multinucleated giant cells in the epidermis, thickening and cellular edema of epithelia in the hair follicles, and vascular dilatation in the papillary plexus. Measles virus antigen was detected by ABC immunoperoxidase in the epidermis, follicular epithelia, and lympho‐histiocytic cell infiltrates in the upper part of the dermis. This rash was deemed to be caused in part by direct viral infection of the epidermal cells.
Interferon‐α (INF‐α) and Etretinate in the Treatment of Mycosis Fungoides
Authors: Torii Hideshi , Kaneko Takehiko , Matsuyama Tomohiko , Nakanishi Hiroshi , Harada Syotaro 
Published: April 2015

AbstractA combination therapy of mycosis fungoides (MF) with interferon‐α (INF‐α) and etretinate was reported. A 53‐year‐old patient suffering from MF was treated with intramuscular administration of INF‐α at a dosage of 9 × 106 IU daily; skin lesions poorly responded to the treatment. After oral etretinate with a dosage of 50 mg daily was combined with INF‐α, marked clinical improvement was seen in skin lesions with minor side effects. INF‐α in combination with etretinate can have beneficial effects in the treatment of MF.
Three Cases of Organoid Nevus on the Trunk and Extremity
Authors: Kanekura Takuro , Kawahira Masakimi , Kanzaki Tamotsu 
Published: April 2015

AbstractOrganoid nevi are most commonly observed on the scalp. Although they are rarely found on the trunk and extremities, we were able to study 3 cases of organoid nevi involving distributions of this sort. These included a 39‐year‐old male with a 2 × 6 cm brown area on the left flank (case 1), an 8‐year‐old female with a 5 × 6 cm hypopigmented macule on the lower left leg (case 2), and a 45‐year‐old male with a 1.5 × 3 cm brown spot on the back (case 3). All these were histopathologically confirmed to be organoid nevi.The lesion in case 1 revealed basal cell epithelioma, that in case 2 exhibited hypohidrosis caused by poorly developed sweat glands, and the lesion in case 3 was typical, as was an additional one on the same patient's scalp.
A Case of Giant Cell Tumor of the Tendon Sheath Developing on the Palm
Authors: Mizushima Junichi , Nogita Toshitatsu , Ooe Mariko , Kawashima Makoto 
Published: April 2015

AbstractA 22‐year‐old Japanese woman with a giant cell tumor of the tendon sheath on the palm is described. The location of the tumor is rare. Light microscopically, the tumor consisted of round or polygonal cells, giant cells, and foam cells. Electron microscopically, histiocyte‐like cells and fibroblast‐like cells were mainly observed, suggesting that the tumor may have originated from the synovial membrane.
Prognostic Evaluation of Cutaneous Malignant Melanoma Based on the pTNM Classification
Authors: Sugihara Tsuneki , Yoshida Tetsunori , Kokubu Ichiro , Chiba Osamu , Ohura Takehiko , Ohkawara Akira 
Published: April 2015

AbstractThe prognoses of 100 consecutive melanoma patients were analyzed on the basis of Breslow's thickness and Clark's levels as well as according to stage employing the 1987 UICC pTNM classification. Among patients with lesions ≦1.50 mm thick, the 10‐year survival rate was 100% for both pT1 (n=13) and pT2 (n=6) disease, 73.4% for pT3a disease (n=26), 62.2% for pT3b disease (n=15), 69.3% for pT3 (n=41) disease, and 38.7% for pT4 disease (n=38). Significant differences in survival were found between the pT4 group and the pT1/pT3a or pT3 groups. The 10‐year survival rate was 100% for level II (n=13) and level III (n=13) disease, 58.3% for level IV (n=46) disease, and 34.5% for level V (n=26) disease. Significant differences were found between level V and other levels. The survival rate at 10 years was 100% for stage I (n=18), 63.3% for stage II (n=24), and 52.5% for stage III (n=55). In stage IV (n=3), there was only one patient who survived for 42 months. There were significant differences in survival among all stages except I and II. The 10‐year survival rate in 3 subgroups of stage III was 58.9% for pT4pN0M0 patients (n=14), 63.2% for pT,pN1M0 patients (n=31), and 20% for pT,pN2M0 patients (n=10). Significant differences were found between the pT,pN1M0 and pT,pN2M0 subgroups. These findings indicate that the survival of pT1, pT2 and stage I patients is extremely good. In contrast, the prognosis of patients with pT3, pT3b or pT4, and stage III or IV disease is significantly poor. Within stage III, the prognosis of the pT,pN2M0 subgroup is significantly poor, and the classification of pN2 patients may need to be reconsidered.
Minocycline Hydrochloride Hyperpigmentation Complicating Treatment of Pyoderma Gangrenosum
Authors: Miralles Enrique S. , Nuñez María , Pérez Bibiana , Ledo Antonio 
Published: April 2015

AbstractMinocycline‐associated hyperpigmentation is an uncommon side effect We report the case of a patient with pyoderma gangrenosum successfully treated with oral minocycline but complicated by marked hyperpigmentation in his pyoderma gangrenosum and acne scars. One of the clinical forms of minocycline hyperpigmentation includes dark‐blue or black macules in depressed acne scars or other sites of skin inflammation; this pattern seems to be independent of the total cumulative dose and the skin process.
Conversion of Antinuclear Antibody Specificity as a Marker of Deterioration of Cutaneous Lupus Erythematosus into Lupus Nephritis
Authors: Kihira Chika , Mizutani Hitoshi , Shimizu Masayuki 
Published: April 2015

AbstractA 34‐year‐old male systemic lupus erythematosus patient (SLE) with cutaneous vasculitis developed renal failure after switching anti‐nuclear antibody (ANA) specificity. He developed cutaneous lupus erythematosus with homogeneous and speckled type ANA and a high titer of anti‐DNA antibody without renal involvement at 21 years of age. After developing lupus nephritis at the age of 27, the original ANA disappeared gradually. Two years later, a discrete speckled type ANA titer elevated abruptly to as high as 1:640 with low complementemia and without DNA antibody. Within five years, he suffered renal failure. This case of SLE suggests a direct correlation with ANA pattern and organ involvement.
A Case of Cutaneous Infection by Exophiala jeanselmei
Authors: Hayashi Miki , Kiryu Hiroe , Suenaga Yoshinori , Asahi Masakazu 
Published: April 2015

AbstractA case of cutaneous infection by Exophiala jeanselmei is reported.The patient was a 45‐year‐old Japanese female. Her skin eruption consisted of a group of erythematous papules on her left cheek to which corticosteroid had been topically applied for nearly 10 years. Histopathologically, a granuloma formation was noted in the dermis within which were many brown spores. The isolated fungus was identified as Exophiala jeanselmei.
Healing of Chronic Atopic Dermatitis Lesions in Skin Areas of Paraplegia after Trauma
Authors: Amon Ulrich , Wolff Helmut H. 
Published: April 2015

AbstractA case of atopic dermatitis in a 12‐year‐old boy with spontaneous clearing of skin lesions in areas of paraplegia after trauma is reported. This observation might confirm the important pathogenetic role of neuropeptides in the development of atopic dermatitis.
Coexistence of Pemphigus and Bullous Pemphigoid
Authors: Matsubara Kunihiko , Kanauchi Hideo , Tanaka Toshihiro , Imamura Sadao 
Published: April 2015

AbstractWidespread tense blisters developed on a 60‐year‐old Japanese man who had been diagnosed with pemphigus 11 years earlier, because of a history of pruritic erythema and erosions on his face, chest, and back, mild supra‐basal layer blister formation found in a biopsy specimen, and a positive direct immunofluorescence test showing IgG deposition in the intercellular space. The histological findings showed subepidermal blister, and the immunoblot study detected 180kD bullous pemphigoid antigen. Direct immunofluorescence test revealed intercellular staining for IgG, and indirect immunofluorescence tests repeatedly demonstrated the presence of circulating antibodies to the intercellular space. From these observations, this case suggests the coexistence of pemphigus and bullous pemphigoid.
CD44 Expression in Normal Human Skin and Skin Tumors
Authors: Yasaka Nami , Furue Masutaka , Tamaki Kunihiko 
Published: April 2015

AbstractCD44 is thought to be a principal cell surface receptor for hyaluronic acid. Although the distribution of hyalulonic acid has been studied, little is known about the distribution of the CD44 molecule in the human skin and skin tumors. This study was undertaken to investigate the distribution of the CD44 molecule in normal human skin as well as in benign and malignant skin tumors.In normal skin, CD44 was expressed on 1) keratinocyte cell surfaces throughout the epidermis except for the granular and horny layers, 2) hair follicular cells, 3) eccrine sweat gland cells, and 4) cell surfaces of dendritic cells in the dermis. In skin tumors, although CD44 was expressed on the tumor cell surface of seborreic keratosis, Bowen's disease, and squamous cell carcinoma as in normal skin, we could not detect any CD44 expression on the cell surface of the tumor cells of basal cell carcinoma. However, CD44 positive dendritic cells were observed in the tumor islands of basal cell carcinoma. Phenotypic analysis suggested that these CD44 positive cells were melanocytes.
A Histological Study of Cutaneous Thermal Wounds Using a Clostridium perfringens‐Derived Wound Healing Substance with Wound Healing Stimulation Activity
Authors: Takahashi Makoto , Morimoto Yousuke , Kon Miyuki , Saga Kenji 
Published: April 2015

AbstractWe studied the effects of a Clostridium perfringens‐derived wound‐healing substance (WHS) on the healing of thermal burn wounds. Third‐degree burn injuries were inflicted on the back skin of rats. We histologically evaluated the effects of WHS ointments and compared them with those of lysozyme chloride ointment. We observed the formation of dermal collagen fibers and the increase of capillaries in the WHS ointment treated groups. From the results of hematoxylin and eosin staining and silver staining, an increase in capillaries was observed one week after the application of WHS ointment. Three weeks after the application, when the epithelization was in the final stage, capillary formation ceased. In the WHS ointment‐applied groups, electron microscopic observation showed that new collagen fibers were regularly formed in the dermis. On the other hand, in the lysozyme chloride ointment‐applied groups, new collagen fibers were present, but were irregularly formed. The main wound healing stimulative action of the WHS ointment could be due to its acceleration of new capillary formation.
A Case of Trichorrhexis Nodosa Developed in Winter
Authors: Bhutto Abdul Manan , Taira Kayo , Nagamine Yasushi , Maruno Motoyoshi , Takamiyagi Atsushi , Nonaka Shigeo 
Published: April 2015

AbstractA young Japanese man developed localized trichorrhexis nodosa (LTN) of the scalp hair in the winter season. To investigate the roles of shampoo, severe sunlight exposure and/or mechanical injuries, we performed the following studies. Hair was collected from the patient and from a control. The study was performed in two steps. In the first step, hair was put into shampoo, rinsed with saline water, and then exposed to ultraviolet B (UVB) radiation once a day for one week. In the second step, the hair was similarly treated, but each shaft was bent gently with forceps before UVB exposure. Scanning electron microscopic studies revealed cuticular changes when the hair was treated only with shampoo and UVB. When it was treated with shampoo, UVB, and mechanical bending, the patient's hair developed longitudinal and transverse fractures of the hair shafts, while the control hair showed only partial damage to the hair shaft. On the basis on the above findings, we conclude that mechanical bending may damage the hair shaft.
Neutrophilic Eccrine Hidradenitis: Report of Two Cases
Authors: Kanzaki Hiroko , Oono Takashi , Makino Eiichi , Arata Jirô , Oda Megumi , Akazai Ayumi 
Published: April 2015

AbstractBackgroundNeutrophilic eccrine hidradenitis (NEH) is an uncommon, self‐limited dermatosis usually attributed to anti‐cancer chemotherapy. It is characterized histologically by necrosis of the eccrine gland and neutrophilic infiltrate.ObservationsWe saw NEH in a 5‐year‐old boy with acute lymphoblastic leukemia and a 4‐year‐old girl with acute monocytic leukemia. NEH developed after the anti‐leukemic chemotherapy including high dose cytarabine. The eruption was composed of vesicles, papules, and plaques.ConclusionsHistological findings were compatible with those described in the literature. NEH in our two patients could be attributed to high doses of cytarabine.
Activity of Eight Fluoroquinolones against Both Methicillin‐Susceptible and ‐Resistant Staphylococcus aureus Isolated from Skin Infections
Authors: Nishijima Setsuko , Namura Shoko , Akamatsu Hirohiko , Kawai Shuzou , Asada Yasuo , Kawabata Shigekatsu , Fujita Maasa 
Published: April 2015

AbstractThe in vitro susceptibility of Staphylococcus aureus to eight fluoroquinolones, norfloxacin, ofloxacin, enoxacin, ciprofloxacin, lomefloxacin, tosufloxacin, sparfloxacin, and nadifloxacin was established by agar dilution tests, 71 isolates of methicillin‐susceptible (MSSA) and 74 isolates of ‐resistant S. aureus (MRSA) isolated from skin infections. Among all of the fluoroquinolones, nadifloxacin exhibited the lowest MIC for both MSSA and MRSA. In addition, there were no resistant S. aureus, neither MSSA and MRSA, to nadifloxacin. With the exception of nadifloxacin, the incidence of MRSA resistant to fluoroquinolones has gradually increased in recent years. Over half of the MRSA strains were resistant to norfloxacin, ofloxacin, enoxacin, ciprofloxacin, and lomefloxacin.
Further Immunocytochemical Characterization of Cultured Hair Apparatus Cells
Authors: Tanigaki‐Obana Noriko , Nakaoji Kouichi , Ito Masaaki 
Published: April 2015

AbstractIn immunocytochemical studies of cultured hair apparatus cells we employed anti‐hair keratin monoclonal antibodies (HKN‐2, HKN‐4, HKN‐5, HKN‐6 and HKN‐7) and compared the results with electron microscopic studies. On day 1, the cultured cells positively stained with HKN‐2 (52%), HKN‐4 (50%), HKN‐5 (43%), HKN‐6 (33%), or HKN‐7 (37%). On day 3, more than 88% of the cells showed no reaction to any antibody. On day 6, most of the cells stained with all of the antibodies: HKN‐2 (96%), HKN‐4 (92%), HKN‐5 (80%), HKN‐6 (72%) and HKN‐7 (74%). These reactivities were maintained until day 13. Electron microscopic studies revealed that, on day 1, half of the observed cells were immature and 47% of them were already differentiated. Most (88%) of the cells showed immature features and the percentage of differentiated cells had decreased by day 3. The differentiated cells (87%) reappeared by day 6, and degenerated cells (63%) increased by day 13. These immunocytochemical results were consistent with those of simultaneous electron microscopic studies, demonstrating that immature cells proliferated and differentiated into subpopulations of each hair apparatus layer type, including the outer root sheath cells, in this culture system.
So‐Called Mixed Tumor of the Skin on the Wrist: An Immunohistochemical Study
Authors: Torii Hideshi , Nakagawa Hidemi , Ishibashi Yasumasa 
Published: April 2015

AbstractA case of so‐called mixed tumor of the skin arising on the wrist was reported. Immunohistochemical staining of keratins of several molecular weights (AE1/AE3, RCK102, NCL‐5D3 and 35βH11), carcinoembryonic antigen (CEA), S‐100 protein, and desmin was performed. AE1/AE3 and RCK102 were positive in all the tumor cells; CEA, NCL‐5D3 and 35βH11 were positive mainly in luminal cells of the tubuloalveolar structures. S‐100 protein was positive in peripheral cells of the tubular lumina and in scattered cells in the mucous stroma. Desmin was negative in all the tumor cells. Immunohistochemical findings lent further support to the hypothesis that so‐called mixed tumor of the skin differentiates into the secretory and ductal portions of the sweat gland.
Lymphoepithelioma‐like Carcinoma Originating on the Eyelid
Authors: Maruyama Misuzu , Miyauchi Shunji , Ohtsuka Hisashi , Miki Yoshiharu 
Published: April 2015

AbstractAn 89‐year‐old woman presented a rapidly growing red nodule of 5 years' duration on her left eyelid. Histologically, the entire dermis was occupied by multiple lobules of atypical tumor cell nests surrounded by inflammatory cells and fibrous stroma. The tumor cell nests were connected with the overlying epidermis and extended into the subcutaneous fat and muscles. There were no tendencies towards squamous or glandular differentiation of the tumor cells. Immunohistochemistry showed the tumor cells to be positive for keratin/cytokeratin and epithelial membrane antigen, but negative for neuron specific enolase and vasoactive intestinal polypeptide. S‐100 protein‐positive Langerhans cells were also found within the tumor nests. There was no apparent evidence of a primary lesion elsewhere.