bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of SSR markers for the tropical alpine tree species Leptospermum recurvum (Myrtaceae) on Mount Kinabalu in Borneo
Authors: Ando Soichi , Kaneko Shingo , Isagi Yuji , Repin Rimi , Kitayama Kanehiro 
Published: September 2013

• Premise of the study: Nuclear microsatellite (simple sequence repeat [SSR]) markers were developed for the woody species Leptospermum recurvum found on Mount Kinabalu, Borneo, to facilitate investigation of the genetic structure and patterns of gene flow in relation to leaf phenotypic polymorphisms.• Methods and Results: Eleven primer pairs were developed using the compound SSR marker technique. Ten of the 11 loci were polymorphic and their expected heterozygosity ranged from 0.04 to 0.83. Neither linkage disequilibrium nor departure from Hardy–Weinberg equilibrium were detected. All primer pairs also amplified the SSR loci of L. polygalifolium.• Conclusions: These findings suggest the utility of these primers for investigating genetic structure and gene flow in L. recurvum and indicate their applicability to another species of Leptospermum.
Development and characterization of microsatellite markers for the endangered Amazonian tree Aniba rosaeodora (Lauraceae)
Authors: Angrizani Rafael C. , Contim Luis A. S. , Lemes Maristerra R. 
Published: September 2013

• Premise of the study: Microsatellite loci were isolated and characterized for Brazilian rosewood (Aniba rosaeodora), an endangered neotropical hardwood tree, to investigate population and conservation genetics of this highly valuable nontimber forest resource.• Methods and Results: We used an enriched genomic library method to isolate and characterize 11 nuclear microsatellite loci for A. rosaeodora, which exhibited an average of 9.6 and 8.7 alleles per locus in two populations from central Amazonia. Mean observed and expected heterozygosities over the 11 loci were 0.604 and 0.687, and 0.807 and 0.828, respectively, in the two populations.• Conclusions: The polymorphic microsatellite loci developed for A. rosaeodora showed highly informative content and can be used as a powerful tool in genetic diversity and population structure, gene flow, and mating system studies for conservation purposes.
Isolation and characterization of microsatellite loci for Cornus sanguinea (Cornaceae)
Authors: Wadl Phillip A. , Hatmaker E. Anne , Fussi Barbara , Scheffler Brian E. , Trigiano Robert N. 
Published: September 2013

• Premise of the study: To facilitate genetic and conservation research of Cornus sanguinea, microsatellite loci were isolated and 29 individuals from 11 German populations were genotyped.• Methods and Results: Sixteen microsatellite loci were characterized from an enriched small insert genomic library. The number of alleles detected ranged from five to 11 per locus, observed heterozygosity ranged from 0.00 to 1.00, expected heterozygosity ranged from 0.65 to 0.90, and polymorphic information content ranged from 0.59 to 0.88.• Conclusions: The markers described in the study will allow further investigation of population dynamics and the degree of clonal reproduction within populations of C. sanguinea.
Development and characterization of microsatellite markers for Lilium longiflorum (Liliaceae)
Authors: Sakazono Satomi , Hiramatsu Michikazu , Watanabe Masao , Okubo Hiroshi 
Published: September 2013

• Premise of the study: Ten microsatellite primers were developed to obtain information on genetic variation in Lilium longiflorum, a bulbous species showing high intraspecific genetic differentiation.• Methods and Results: Of 61 microsatellite loci isolated using the dual suppression PCR technique, 10 loci were effective to characterize and estimate genetic variation in two populations of L. longiflorum. The number of alleles at each locus was different between the populations (averages = 3.2 and 10.3 alleles per locus), and the mean observed heterozygosity values were 0.245 and 0.732.• Conclusions: Our results demonstrate that there is significant genetic variation between the populations and that the microsatellite markers developed in this study will be useful tools for the investigation of the genetic structure and mating system of natural L. longiflorum populations.
Microsatellite primers for the Pacific Northwest conifer Callitropsis nootkatensis (Cupressaceae)
Authors: Jennings Tara N. , Knaus Brian J. , Alderman Katherine , Hennon Paul E. , D'Amore David V. , Cronn Richard 
Published: September 2013

• Premise of the study: Microsatellite primers were developed for Nootka cypress (Callitropsis nootkatensis) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate‐induced decline.• Methods and Results: Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite‐containing sequences from 489,625 Illumina paired‐end 80‐bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of C. nootkatensis. Loci show between three and 36 dinucleotide repeats per locus, with an average of 13. Screening of these markers in the Pacific Northwest relative Chamaecyparis lawsoniana demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in Callitropsis.• Conclusions: These microsatellites show high polymorphism and can be used for routine screening of natural variation in Callitropsis nootkatensis, and will be particularly helpful in identifying clones and inbred relatives at the stand‐level.
Very‐high‐resolution time‐lapse photography for plant and ecosystems research
Authors: Nichols Mary H. , Steven Janet C. , Sargent Randy , Dille Paul , Schapiro Joshua 
Published: September 2013

• Premise of the study: Traditional photography is a compromise between image detail and area covered. We report a new method for creating time‐lapse sequences of very‐high‐resolution photographs to produce zoomable images that facilitate observation across a range of spatial and temporal scales.• Methods and Results: A robotic camera mount and software were used to capture images of the growth and movement in Brassica rapa every 15 s in the laboratory. The resultant time‐lapse sequence (http://timemachine.gigapan.org/wiki/Plant_Growth) captures growth detail such as circumnutation. A modified, solar‐powered system was deployed at a remote field site in southern Arizona. Images were collected every 2 h over a 3‐mo period to capture the response of vegetation to monsoon season rainfall (http://timemachine.gigapan.org/wiki/Arizona_Grasslands).• Conclusions: A technique for observing time sequences of both individual plant and ecosystem response at a range of spatial scales is available for use in the laboratory and in the field.
Can Effluent Matrix Metalloproteinase 2 and Plasminogen Activator Inhibitor 1 be Used as Biomarkers of Peritoneal Membrane Alterations in Peritoneal Dialysis Patients?
Authors: Barreto Deirisa Lopes , Coester Annemieke M. , Struijk Dirk G. , Krediet Raymond T. 
Published: September 2013

Background Peritoneal effluent contains clinically relevant substances derived from intraperitoneal production or transperitoneal transport, or both. The glycoproteinase matrix metalloproteinase 2 (MMP-2) cleaves denatured collagen and complements other collagenases in the degradation of fibrillar collagens. Elevated intraperitoneal levels of plasminogen activator inhibitor 1 (PAI-1) have been demonstrated to be present in patients with intra-abdominal adhesions. The aim of the present study was therefore to investigate the potential for effluent MMP-2 and PAI-1 to be used as markers of the development of peritoneal alterations. In addition, MMP-2 was analyzed in previously frozen effluent samples from a uremic rat model, in which data concerning the severity of peritoneal fibrosis were available. Methods This prospective, single-center cohort study included 86 incident peritoneal dialysis (PD) patients. All patients were treated solely with biocompatible dialysis solutions and underwent a standard peritoneal permeability analysis (SPA). The presence of local MMP-2 and PAI-1 production and the relationships between those markers and peritoneal transport parameters were analyzed. Furthermore, effluent interleukin 6 was analyzed as a marker of local inflammation. Results Median effluent levels of 21.4 ng/mL for MMP-2 and 0.9 ng/mL for PAI-1 were found. The median dialysate appearance rates were 218.8 ng/min for MMP-2 and 9.6 ng/min for PAI-1. Local peritoneal production averaged 90% of effluent MMP-2 concentration and 74% of effluent PAI-1 concentration. Furthermore, correlations between peritoneal transport parameters and MMP-2 and PAI-1 were observed. Longitudinal follow-up showed no change for MMP-2 ( p = 0.37), but a tendency for PAI-1 to increase with the duration of PD ( p < 0.001). In rats, a significant relationship was present between the extent of peritoneal fibrosis and the appearance rate of MMP-2 ( r = 0.64, p = 0.03). Conclusions The foregoing data illustrate the potential of effluent MMP-2 and PAI-1 as biomarkers of peritoneal modifications, especially fibrosis; however, the components of peritoneal transport and local production should be clearly distinguished in every patient.
Inflammation and Hypertension in Rheumatoid Arthritis
Authors: Manavathongchai Siriporn , Bian Aihua , Rho Young Hee , Oeser Annette , Solus Joseph F. , Gebretsadik Tebeb , Shintani Ayumi , Stein C. Michael 
Published: September 2013

Objective.Hypertension (HTN), a common modifiable cardiovascular risk factor, is more common in patients with rheumatoid arthritis (RA), but the underlying mechanisms are unclear. We examined the hypothesis that mediators of inflammation and markers of cardiovascular risk are associated with HTN in RA.Methods.We compared measures of inflammation [serum C-reactive protein (CRP), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), homocysteine, and leptin concentrations] and insulin resistance [homeostasis model assessment index (HOMA)] in RA patients with (n = 90) and without HTN (n = 79). HTN was defined as blood pressure ≥ 140/90 mm Hg or treatment with antihypertensive therapy. The independent association of markers of interest with HTN was examined using multivariable logistic regression.Results.Patients with HTN were significantly older and had longer disease duration than those without HTN (both p < 0.001). Concentrations of homocysteine [11.1 (8.5–13.5) μmol/l vs 9.3 (7.8–11.0) μmol/l] were significantly higher in patients with HTN (p < 0.001). After adjustment for age, sex, race, smoking, body mass index, and corticosteroid and nonsteroidal antiinflammatory drugs (NSAID) use, increased concentrations of homocysteine (OR 2.9, 95% CI: 1.5–5.5, p = 0.001), and leptin (OR 2.0, 95% CI: 1.0–3.8, p = 0.046) were significantly associated with HTN, but the 28-joint Disease Activity Score, IL-6, CRP, TNF-α, and HOMA index were not (all p > 0.05).Conclusion.HTN in patients with RA is not associated with generalized systemic inflammation or insulin resistance, but is associated with increasing concentrations of homocysteine and leptin. The pathogenesis of HTN in RA may involve pathways more regularly associated with fat and vascular homeostasis.
Assessment of acquired immune response to <i>Rhipicephalus appendiculatus</i> tick infestation in different goat breeds
Authors: Gopalraj Jeyanthi B.P. , Clarke Francoise C. , Donkin Edward F. 
Published: September 2013

Changes in serum gamma globulin levels, numbers of replete female ticks and engorged tick mass were used as parameters to monitor the acquired immune response (antibody mediated immune response) elicited by Rhipicephalus appendiculatus adult tick infestations. Three consecutive Rhipicephalus appendiculatus adult tick infestations were applied to South African Indigenous goats (Nguni), Saanen goats and cross-bred goats (Saanen goats crossed with South African Indigenous goats [Nguni]) under laboratory conditions. During the three consecutive Rhipicephalus appendiculatus adult tick infestations the serum gamma globulin levels increased in all three breeds, whilst the mean replete female tick numbers and engorged tick mass decreased. Even though all three goat breeds exhibited an acquired immune response, the South African Indigenous goats (Nguni) response was significantly higher than that of the Saanen and cross-bred goats. However, the acquired immune response elicited by Saanen goats was significantly lower when compared with cross-bred goats.
Pregnancy Outcome in Viral Hepatitis
Authors: Joshi Sulbha , Kose Varsha 
Published: September 2013

ABSTRACT

Introduction
Hepatitis in pregnancy is an important medical disorder seen more often in developing countries than in developed ones. Viral hepatitis is a major public health problem in India. Hepatitis in pregnancy is commonly associated with abortion, premature delivery, postpartum hemorrhage, coagulation defect, obstetric shock, coma, death and increased perinatal mortality and morbidity. This study was carried out to know the effect of viral hepatitis on pregnancy and to study its maternal and fetal outcome.

Materials and methods
This prospective clinical study was conducted in the Department of Obstetrics and Gynecology NKP Salve Institute of Medical Sciences and Research Centre and Lata Mangeshkar Hospital, Nagpur, from January 2010 to December 2012. All the antenatal cases attended Obstetrics and Gynecology Department were investigated for HBsAg and looked for any symptoms and signs suggesting hepatitis like fever, jaundice, nausea, vomiting. If present, they were admitted and further investigated to detect viral hepatitis. Patients were counseled for follow-up and strict hospital delivery. Patients with viral hepatitis were analyzed for the effect on pregnancy, maternal and fetal outcome.

Results
There were total 30 cases of viral hepatitis 24 (80%) cases were HBsAg positive, but all were asymptomatic 6 (20%) cases were hepatitis E positive. No HAV, HCV, HDV and hepatitis G infected cases were detected in our study. Maternal and fetal morbidity was significantly higher in hepatitis E infected women. Fetal mortality was observed in one case.

Conclusion
Pregnancy with viral hepatitis requires early diagnosis, hospitalization and treatment. In the present study hepatitis E was found to be the chief etiological agent associated with increased maternal morbidity, and high fetal morbidity and mortality.

How to cite this article
Kose V, Joshi S. Pregnancy Outcome in Viral Hepatitis. J South Asian Feder Obst Gynae 2013;5(2): 60-63.
Group B Streptococci Colonization in Pregnant Women: Is Screening Necessary?
Authors: Patil Kamal , Singla SS , Nagmoti MB 
Published: September 2013

ABSTRACT

Objectives
Group B Streptococcus (GBS) has been recognized as the leading cause of serious neonatal infections through mother—fetal vertical transmission in the west, however, in India, its spectrum is largely under estimated. The present study was carried out to find the incidence of rectovaginal carriage of GBS in parturient women, association with risk factors in mother and to study the neonatal outcome.

Materials and methods
A one year cross-sectional prospective study was carried out in 905 parturient women admitted at a tertiary care center meeting the selection criteria over a period of one year from June 2007 to May 2008. The inclusion criteria were all pregnant women admitted to the labor room after 35 ± 1 week of gestation. Rectal and vaginal swabs were taken and cultured on selective Todd-Hewitt broth medium followed by sub culture on blood agar and confirmation by Latex agglutination test from all the women meeting the inclusion criteria. The outcomes measured were the incidence, antenatal risk factors in mother and the neonatal outcome. Analysis was done using paired ‘t’ test, chi-square test and a p-value of <0.05 was taken as statistically significant.

Results
Incidence of group B Streptococcus (GBS) was 12.15% and detection rate was increased by 4.6% with the inclusion of rectal swabs for culture. GBS carriage was significantly increased with preterm birth (OR 8.3, 95% CI,1.1- 15.5), premature rupture of membranes (OR 7.5, 95% CI, 1.1- 13.4), prolonged duration of ruptured membranes more than 10 hours (OR 21, 95% CI,15.2-34.2) and intrapartum temperature more than 38°C(OR 3.1, 95% CI, 0.43-6.66). Birth weight less than 2.5 kg and neonatal intensive care admissions were significantly more (35.45%) in infants of GBS positive women.

Conclusion
GBS colonization was more frequent in women with risk factors. GBS pick up rate was increased by the inclusion of both rectal and vaginal swabs.

How to cite this article
Patil KP, Singla SS, Nagmoti MB, Swamy MK. Group B Streptococci Colonization in Pregnant Women: Is Screening Necessary? J South Asian Feder Obst Gynae 2013;5(2):64-67.
Improving Postabortion Care: Reflections from Sri Lanka
Authors: Priyanwada Pathiraja Ramya , Lakshmen Senanayake 
Published: September 2013

ABSTRACTWomen who have experienced complications from miscarriage and unsafely induced abortions are among the most neglected of reproductive healthcare patients. In Sri Lanka and, the highest abortion rate of 67/1000 was recorded among women in the 35 to 39 years age. Long-term consequences, such as chronic pain, pelvic inflammatory disease and tubal occlusion, leading to secondary subfertility may result following abortions, and medical treatment remains a crucial component of care. Delay in seeking care, when complications occur, is an important contributory factor leading to death of these patients.The infrastructure to make PAC services widely available usually lack in most of the developing countries. Emotional support, psychosocial counseling and provision of other reproductive services when necessary, following abortion have been shown to improve women's reproductive health and quality of their lives.Care providers need to be supportive and should ensure the confidentiality and there is a need to strengthen the existing contraception service delivery system. PAC services should be continuously reviewed and appropriate changes should be made whenever necessary. Implementation is hindered by lack of awareness of national policies, overburdened and understaff, lack of staff skilled in counseling and supervision and poor contraceptive logistics.Working in partnership, regular training, incorporation of PAC in preservice curricula is important to achieve universal access to sustainable high-quality PAC.How to cite this articlePriyanwada PR, Lakshmen S. Improving Postabortion Care: Reflections from Sri Lanka. J South Asian Feder Obst Gynae 2013;5(2):72-75.
Indian Clinical Experience on Innovative Product in Treatment of Male Infertility
Authors: Biswas KP , Amin Jayesh , Dutta Asit Kumar , Kunwar Kumud , Shinde Veena G , Chauhan Archana , Sud Shilpi , Maurya Rashmi , Nepal Neeti , Sarode Pankaj , Mazumder GP , Chitrabanshi Maulshri , Shibe Sushma , Shukla Ruma , Trivedi Neeraj , Dayal Meena , Sortey Devendra , Shukla Sharvaree , Gawade Bharati 
Published: September 2013

ABSTRACT
Rising number of infertile males in India provokes the need of evidence-based comprehensive formula addressing unattended causes of male infertility. There is an innovative product*, a combikit available in Indian market, containing 30 tablets of antioxidant, multivitamin, amino acid and mineral tablets, with documented evidence on ingredients in treatment of male infertility, and 25 tablets of clomiphene citrate which is a timetested drug in treatment of male infertility (in particular oligospermia and/or asthenospermia). In order to assess efficacy of the product in Indian infertile male patients, we undertook phase IV multicentric clinical study, at 18 centers across India in 100 patients.
Very high couple pregnancy rate of 53% was reported. As first line treatment of male infertility (in particular oligospermia and/or asthenospermia), the use of this innovative product therefore holds a strong promise.

How to cite this article
Amin J, Mishra SK, Dutta AK, Kunwar K, Shinde VG, Chauhan A, Sud S, Maurya R, Nepal N, Sarode P, Biswas KP, Mazumder GP, Chitrabanshi M, Shibe S, Shukla R, Trivedi N, Dayal M, Sortey D, Shukla S, Gawade B. Indian Clinical Experience on Innovative Product in Treatment of Male Infertility. J South Asian Feder Obst Gynae 2013;5(2):76-79.
Vaginal Adenosis in a 40-year-old Lady: A Case Report and Review of Literature
Authors: Hena Shamsun Nahar Begum , Akhter Nasrin , Das Sakti , Kutubi Afroza 
Published: September 2013

ABSTRACT
Vaginal adenosis, without a history of diethylstilbestrol (DES) exposure, is a rare condition with an unclear etiology. A 40- year-old female presented with complaints of persistent excessive watery vaginal discharge. On examination, there was red, patchy, diffuse lesion all over the vaginal wall and cervix. Histopathological examination of the lesion revealed vaginal adenosis. This case is presented here for its rarity.

How to cite this article
Hena SNB, Akhter N, Das S, Kutubi A. Vaginal Adenosis in a 40-year-old Lady: A Case Report and Review of Literature. J South Asian Feder Obst Gynae 2013;5(2):80-82.
A Rare Case Report of Prune Belly Syndrome
Authors: Singhania Pooja , Shrivastava Deepti , Kakani Anuradha , Papalkar Juhee 
Published: September 2013

ABSTRACT
Prune belly syndrome is a congenital abnormality of unknown etiology with characteristic features: Deficient development of abdominal muscles that causes the skin of the abdomen to wrinkle like a prune, cryptorchidism, abnormalities of the urinary tract. It is associated with high incidence of fetal and neonatal mortality. It can be diagnosed at 16 weeks during routine ultrasound and can be managed by fetal therapy. In rural setup women are neither visiting for routine anomaly scan nor affording for fetal therapy, hence termination of pregnancy remains the only option.

How to cite this article
Papalkar J, Shrivastava D, Kakani A, Singhania P. A Rare Case Report of Prune Belly Syndrome. J South Asian Feder Obst Gynae 2013;5(2):83-84.
Spontaneous Parasitic Leiomyoma: A Rare Clinical Experience
Authors: Mandal Debasmita , Dattaray Chaitalli , Roy Sanchita 
Published: September 2013

ABSTRACT
Although leiomyoma is one of the commonest uterine tumors prevalent among reproductive aged women, certain types like parasitic fibroid are rare and among these the primary variety rarer. The present case report is of a primary parasitic fibroid of large size neither connected with uterus and adnexae nor having any history of previous laparoscopic procedures. Aim of our reporting is to acknowledge the rarity, clinical parameters and management.

How to cite this article
Mandal D, Dattaray C, Roy S. Spontaneous Parasitic Leiomyoma: A Rare Clinical Experience. J South Asian Feder Obst Gynae 2013;5(2):85-86.