bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age Increase of Estrogen Receptor-α (ERα) in Cortical Astrocytes Impairs Neurotrophic Support in Male and Female Rats
Authors: Arimoto Jason M. , Wong Angela , Rozovsky Irina , Lin Sharon W. , Morgan Todd E. , Finch Caleb E. 
Published: March 2013

Abstract
Rodent models show decreased neuronal responses to estradiol (E2) during aging (E2-desensitization) in association with reduced neuronal estrogen receptor (ER)-α, but little is known about age changes of E2-dependent astrocytic neurotrophic support. Because elevated expression of astrocyte glial fibrillary acidic protein (GFAP) is associated with impaired neurotrophic activity and because the GFAP promoter responds to ERα, we investigated the role of astrocytic ERα and ERβ in impaired astrocyte neurotrophic activity during aging. In vivo and in vitro, ERα was increased greater than 50% with age in astrocytes from the cerebral cortex of male rats (24 vs 3 months), whereas ERβ did not change. In astrocytes from 3-month-old males, experimentally increasing the ERα to ERβ ratio induced the aging phenotype of elevated GFAP and impaired E2-dependent neurite outgrowth. In 24-month-old male astrocytes, lowering ERα reversed the age elevation of GFAP and partially restored E2-dependent neurite outgrowth. Mixed glia (astrocytes to microglia, 3:1) of both sexes also showed these age changes. In a model of perimenopause, mixed glia from 9- to 15-month rats showed E2 desensitization: 9-month regular cyclers retained young-like ERα to ERβ ratios and neurotrophic activity, whereas 9-month noncyclers had elevated ERα and GFAP but low E2-dependent neurotrophic activity. In vivo, ERα levels in cortical astrocytes were also elevated. The persisting effects of ovarian acyclicity in vitro are hypothesized to arise from steroidal perturbations during ovarian senescence. These findings suggest that increased astrocyte ERα expression during aging contributes to the E2 desensitization of the neuronal responses in both sexes.
Synthesis and Optical Properties of Some Compounds Based on Carbazole and s-Triazine
Authors: Diao Lixiao , Cui Yuezhi , Li Tianduo , Zhou Longlong , Liu Wenxia 
Published: March 2013

Abstract
A hyperbranched conjugated polymer (P), as well as its repeating units (M), have been synthesized based on 2,4,6-trimethyl-s-triazine and formylcarbazole. Compared with M, P exhibits much stronger fluorescence and a larger Stern–Volmer constant (KSV) in response to DNT, indicating a fluorescence signal amplification effect in the hyperbranched conjugated polymer P.
Tension Tests Behavior of API 5L X60 Pipeline Steel in a Simulated Soil Solution to Evaluate SCC Susceptibility
Authors: Contreras A. , Hernández S. L. , Galvan-Martinez R. , Vega-Becerra O. 
Published: March 2013

ABSTRACTIn this work slow strain rate tests (SSRT) were used for the evaluation of API 5L X60 in contact with a simulated soil solution called NS4 in order to evaluate stress corrosion cracking (SCC) susceptibility. SSRT were carried out in NS4 solution at room temperature to simulate dilute ground water that has been found to be associated with SCC of low carbon steel pipelines. A strain rate of 1x10-6 sec-1 was used. According to the analysis of SSRT results, the X60 pipeline steel is highly resistant to SCC at the conditions studied. A combine fracture type it was observed: ductile and brittle with a transgranular appearance. Some pits close to the fracture zone were observed. The failure process and mechanism of X60 steel in NS4 solution are controlled by anodic dissolution and hydrogen embrittlement which was revealed with the internal cracks observed in the surface fracture. There is a relation between the strength of the steel and the SCC susceptibility, thus, increasing strength in the steel, the SCC susceptibility increases as a function of the pH solution used.
Synthesis of ZnO at Different Atomic Proportion Produced by Chemical Precipitation
Authors: Medina A. , Béjar L. , Herrera-Pérez G. 
Published: March 2013

ABSTRACTZinc oxide (ZnO) nanoparticles were produced using chemical precipitation synthesis with a molar ratio of 1:1, 1:2 and 1:3. The structure, chemical composition and morphology were investigated by X-ray diffraction (XRD), energy dispersive spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and high resolution transmission electron microscopy (HRTEM). XRD and EDS demonstrated that the all particles formed at different atomic proportion were of wurtzite crystal structure with the same chemical composition. SEM and TEM showed the formation of hexagonal particles with a molar ratio of 1:1 while the samples synthesized with a molar ratio 1:2 and 1:3 showed a circular shape. HRTEM and Fast Fourier Transform (FFT) demonstrated that the all particles were formed with a preferable [0001] growth direction.
Junction Operation of GaAs Wire Array Solar Cells
Authors: Halverson Adam F. , Tsakalakos Loucas 
Published: March 2013

ABSTRACTWire array solar cells benefit from enhanced coupling of light into the active area of the device, significantly decreased collection lengths due to radial charge separation and collection, and easier access to grain boundaries for passivation which may enable future deposition on non-wafer substrates. We report on an analysis of the junction operation of wire array based GaAs solar cells through temperature and light intensity dependent current-voltage analysis and compare these data to matched planar devices. We see evidence of non-ideal recombination pathways indicated by activation energies for generation-recombination that are significantly less than the band gap of GaAs. We observe voltage shifts in the wire array devices at low temperature and high light intensity that we posit can be explained by electron accumulation in the window layers of the devices.
Passivation, Separation and Characterization of Plasma Synthesized Silicon Nanoparticles
Authors: Cheng Jifang , Jimenez Catherine , Bell Jacob P. , Anderson Ingrid E. , Kendrick Chito , Yang Yongan , Collins Reuben T. , Williams S. Kim R. 
Published: March 2013

ABSTRACTSilicon nanoparticles (Si NPs) were synthesized by plasma enhanced chemical vapor deposition (PECVD) using silane as a silicon source. Allylamine was used as passivation ligands to form water-soluble Si NPs. Finally, aqueous asymmetric flow field-flow fractionation was used to successfully separate the polydisperse Si NPs into monodisperse Si NP fractions.
The Effect of the Dielectric Constant of a Solution of CdSe Quantum Dots on Electrophoretic Deposition of the Dots
Authors: Smith Nathanael J. 
Published: March 2013

ABSTRACTThe effect on film quality of the dielectric constant εb of the bath solution used for electrophoretic deposition of CdSe quantum dots (QDs) was investigated. Various combinations of solvents yielding different εb were tested, with the best films produced by a bath consisting of hexanes and acetone. Two different types of film were observed, depending on the volume fraction of acetone in the bath: thin, but strongly adhered films for εb < 12 and thick but loose films for εb > 12. This behavior is explained in terms of the rate at which QDs arrive at the surface of the electrode, which depends on the electrophoretic mobility of the QDs and therefore also the dielectric constant of the bath.
Regional Earthquake Likelihood Models I: First-Order Results
Authors: Zechar J. Douglas , Schorlemmer Danijel , Werner Maximilian J. , Gerstenberger Matthew C. , Rhoades David A. , Jordan Thomas H. 
Published: March 2013

Abstract
The Regional Earthquake Likelihood Models (RELM) working group designed a 5-year experiment to forecast the number, spatial distribution, and magnitude distribution of subsequent target earthquakes, defined to be those with magnitude ≥4.95 (M 4.95+) in a well-defined California testing region. Included in the experiment specification were the description of the data source, the methods for data processing, and the proposed evaluation metrics. The RELM experiment began on 1 January 2006 and involved 17 time-invariant forecasts constructed by seismicity modelers; by the end of the experiment on 1 January 2011, 31 target earthquakes had occurred. We analyze the experiment outcome by applying the proposed consistency tests based on likelihood measures and additional comparison tests based on a measure of information gain. We find that the smoothed seismicity forecast by Helmstetter et al., 2007 based on M 2+ earthquakes since 1981, is the best forecast, regardless of whether aftershocks are included in the analysis. The RELM experiment has helped to clarify ideas about testing that can be applied to more wide-ranging earthquake forecasting experiments conducted by the Collaboratory for the Study of Earthquake Predictability (CSEP).
Online Material: Figures and tables showing the RELM testing region and collection region definitions, numerical results associated with the RELM experiment, and the uncorrected forecast by Ebel et al. (2007).
Diabetes‐Associated Periodontitis Molecular Features in Infrared Spectra of Gingival Crevicular Fluid
Authors: Xiang Xiaoming , Duarte Poliana Mendes , Lima Jadson Almeida , Santos Vanessa Renata , Gonçalves Tiago Dias , Miranda Tamires Szeremeske , Liu Kan‐Zhi 
Published: March 2013

Background: It has been established previously that infrared spectroscopy (IRS) can be used to identify periodontitis‐specific molecular signatures in gingival crevicular fluid (GCF) and to confirm clinical diagnoses. This follow‐up study is designed to assess whether this novel technique is also able to differentiate diseased from healthy sites in patients with diabetes mellitus (DM) by analyzing the molecular fingerprints embedded in the GCF.Methods: A total of 65 patients with DM with moderate‐to‐severe chronic periodontitis (CP) was recruited, and 15 individuals without DM (65 sites) without periodontal diseases were used as control. Clinical examination and GCF samples were taken from a total of 351 sites, including periodontitis (109), gingivitis (115), and healthy (127) sites. Corresponding absorption spectra of GCF samples were acquired and processed, and the relative contributions of key functional groups in the infrared spectra were identified and analyzed. The qualitative assessment of clinical relevance of these GCF spectra was interpreted with multivariate statistical analysis: linear discriminant analysis (LDA).Results: Spectral analysis revealed several molecular signatures representing vibrations in protein (amide I and II), lipid ester, and sugar moieties in the GCF of patients with DM with CP and non‐DM controls. The diagnostic accuracy for distinction between healthy and CP sites in patients with DM determined by LDA of GCF spectra was 95.3% for the training set of samples and 87.5% for the validation set. Additional LDA of GCF spectra from healthy sites of non‐DM controls and patients with DM revealed 100% diagnostic accuracy for the training set and 86.7% for the validation set. The regions robotically selected by LDA for the two analyses were slightly different in that first LDA identified major regions clustered with the side chain vibrations originating from protein and DNA contents, whereas the second was predominantly the glycation and protein components.Conclusion: IRS is a feasible method to differentiate disease‐specific molecular signatures in GCF in the presence of DM and to generate a complex biochemical profile of GCF to identify DM‐specific spectral features.
Circulating Lysophosphatidylcholines Are Markers of a Metabolically Benign Nonalcoholic Fatty Liver
Authors: Lehmann Rainer , Franken Holger , Dammeier Sascha , Rosenbaum Lars , Kantartzis Konstantinos , Peter Andreas , Zell Andreas , Adam Patrick , Li Jia , Xu Guowang , Königsrainer Alfred , Machann Jürgen , Schick Fritz , Hrabé de Angelis Martin , Schwab Matthias , Staiger Harald , Schleicher Erwin , Gastaldelli Amalia , Fritsche Andreas , Häring Hans-Ulrich , Stefan Norbert 
Published: March 2013

OBJECTIVE
Nonalcoholic fatty liver (NAFL) is thought to contribute to insulin resistance and its metabolic complications. However, some individuals with NAFL remain insulin sensitive. Mechanisms involved in the susceptibility to develop insulin resistance in humans with NAFL are largely unknown. We investigated circulating markers and mechanisms of a metabolically benign and malignant NAFL by applying a metabolomic approach.

RESEARCH DESIGN AND METHODS
A total of 265 metabolites were analyzed before and after a 9-month lifestyle intervention in plasma from 20 insulin-sensitive and 20 insulin-resistant subjects with NAFL. The relevant plasma metabolites were then tested for relationships with insulin sensitivity in 17 subjects without NAFL and in plasma from 29 subjects with liver tissue samples.

RESULTS
The best separation of the insulin-sensitive from the insulin-resistant NAFL group was achieved by a metabolite pattern including the branched-chain amino acids leucine and isoleucine, ornithine, the acylcarnitines C3:0-, C16:0-, and C18:0-carnitine, and lysophosphatidylcholine (lyso-PC) C16:0 (area under the ROC curve, 0.77 [P = 0.00023] at baseline and 0.80 [P = 0.000019] at follow-up). Among the individual metabolites, predominantly higher levels of lyso-PC C16:0, both at baseline (P = 0.0039) and at follow-up (P = 0.001), were found in the insulin-sensitive compared with the insulin-resistant subjects. In the non-NAFL groups, no differences in lyso-PC C16:0 levels were found between the insulin-sensitive and insulin-resistant subjects, and these relationships were replicated in plasma from subjects with liver tissue samples.

CONCLUSIONS
From a plasma metabolomic pattern, particularly lyso-PCs are able to separate metabolically benign from malignant NAFL in humans and may highlight important pathways in the pathogenesis of fatty liver–induced insulin resistance.
Double-Blind, Randomized Study Evaluating the Glycemic and Anti-inflammatory Effects of Subcutaneous LY2189102, a Neutralizing IL-1β Antibody, in Patients With Type 2 Diabetes
Authors: Sloan-Lancaster Joanne , Abu-Raddad Eyas , Polzer John , Miller Jeffrey W. , Scherer Joel C. , De Gaetano Andrea , Berg Jolene K. , Landschulz William H. 
Published: March 2013

OBJECTIVE
Inflammation is associated with pancreatic β-cell apoptosis and reduced insulin sensitivity. Literature suggests that interleukin (IL)-1β may contribute to the pathogenesis of type 2 diabetes mellitus (T2DM). This study aimed to determine the efficacy, safety, and tolerability of LY2189102, a neutralizing IL-1β antibody, in T2DM patients.

RESEARCH DESIGN AND METHODS
Phase II, randomized, double-blind, parallel, placebo-controlled study of subcutaneous LY2189102 (0.6, 18, and 180 mg) administered weekly for 12 weeks in T2DM patients on diet and exercise, with or without approved antidiabetic medications.

RESULTS
LY2189102 reduced HbA1c at 12 weeks (adjusted mean differences versus placebo: −0.27, −0.38 and −0.25% for 0.6, 18 and 180 mg doses, respectively), and fasting glucose at multiple time points compared with placebo. LY2189102 also reduced postprandial glycemia, and inflammatory biomarkers, including hs-CRP and IL-6. LY2189102 was generally well tolerated.

CONCLUSIONS
Weekly subcutaneous LY2189102 for 12 weeks was well tolerated, modestly reduced HbA1c and fasting glucose, and demonstrated significant anti-inflammatory effects in T2DM patients. Neutralizing IL-1β holds promise as a convenient adjuvant treatment for T2DM.
Type 2 Diabetes and 10-Year Risk of Dementia and Cognitive Impairment Among Older Mexican Americans
Authors: Mayeda Elizabeth R. , Haan Mary N. , Kanaya Alka M. , Yaffe Kristine , Neuhaus John 
Published: March 2013

OBJECTIVE
Type 2 diabetes has been linked with increased risk of dementia and cognitive impairment among older adults and with premature mortality in young and middle-aged adults. No studies have evaluated the association between diabetes and dementia among Mexican Americans, a population with a high burden of diabetes. We evaluated the association of diabetes with incidence of dementia and cognitive impairment without dementia (CIND) among older Mexican Americans while accounting for competing risk from death.

RESEARCH DESIGN AND METHODS
This study included 1,617 participants 60–98 years of age from the Sacramento Area Latino Study on Aging followed up to 10 years from 1998. We evaluated the association between diabetes and dementia/CIND with competing risk regression models.

RESULTS
Participants free of dementia/CIND at baseline (n = 1,617) were followed annually up to 10 years. There were 677 (41.9%) participants with diabetes, 159 (9.8%) incident dementia/CIND cases, and 361 (22.3%) deaths. Treated and untreated diabetes (hazard ratio 2.12 [95% CI 1.65–2.73] and 2.15 [1.58–2.95]) and dementia/CIND (2.48 [1.75–3.51]) were associated with an increased risk of death. In models adjusted for competing risk of death, those with treated and untreated diabetes had an increased risk of dementia/CIND (2.05 [1.41–2.97] and 1.55 [0.93–2.58]) compared with those without diabetes.

CONCLUSIONS
These findings provide evidence that the association between type 2 diabetes and dementia/CIND among Mexican Americans remains strong after accounting for competing risk of mortality. Treatments that modify risk of death among those with diabetes may change future dementia risk.
Baseline Markers of Inflammation Are Associated With Progression to Macroalbuminuria in Type 1 Diabetic Subjects
Authors: Lopes-Virella Maria F. , Baker Nathaniel L. , Hunt Kelly J. , Cleary Patricia A. , Klein Richard , Virella Gabriel ,   the DCCT/EDIC Research Group
Published: March 2013

OBJECTIVE
The current study aimed to determine in the Diabetes Control and Complications Trial (DCCT)/Epidemiology of Diabetes Interventions and Complications cohort whether or not abnormal levels of markers of inflammation and endothelial dysfunction measured in samples collected at DCCT baseline were able to predict the development of macroalbuminuria.

RESEARCH DESIGN AND METHODS
Levels of inflammation and endothelial cell dysfunction biomarkers were measured in 1,237 of 1,441 patients enrolled in the DCCT study who were both free of albuminuria and cardiovascular disease at baseline. To test the association of log-transformed biomarkers with albuminuria, generalized logistic regression models were used to quantify the association of increased levels of biomarkers and development of abnormal albuminuria. Normal, micro-, and macroalbuminuria were the outcomes of interest.

RESULTS
In the logistic regression models adjusted by DCCT treatment assignment, baseline albumin excretion rate, and use of ACE/angiotensin receptor blocker drugs, one unit increase in the standardized levels of soluble E-selectin (sE-selectin) was associated with an 87% increase in the odds to develop macroalbuminuria and one unit increase in the levels of interleukin-6 (IL-6), plasminogen activator inhibitor 1 (PAI-1; total and active), and soluble tumor necrosis factor receptors (TNFR)-1 and -2 lead to a 30–50% increase in the odds to develop macroalbuminuria. Following adjustment for DCCT baseline retinopathy status, age, sex, HbA1c, and duration of diabetes, significant associations remained for sE-selectin and TNFR-1 and -2 but not for IL-6 or PAI-1.

CONCLUSIONS
Our study indicates that high levels of inflammatory markers, mainly E-selectin and sTNRF-1 and -2, are important predictors of macroalbuminuria in patients with type 1 diabetes.
Evaluation of fiber concentration in dry and canned commercial diets formulated for adult maintenance or all life stages of dogs by use of crude fiber and total dietary fiber methods
Authors: Farcas Amy K. , Larsen Jennifer A. , Fascetti Andrea J. 
Published: March 2013

Abstract
Objective—To assess differences among reported maximum crude fiber (CF), measured CF, and measured total dietary fiber (TDF) concentrations, and determine fiber composition in dry and canned nontherapeutic diets formulated for adult maintenance or all life stages of dogs.
Design—Prospective cross-sectional study.
Sample—Dry (n = 20) and canned (20) nontherapeutic canine diets.
Procedures—Reported maximum CF concentrations were obtained from product labels. Concentrations of CF and TDF were measured in samples of the diets for comparison. For each diet, percentages of TDF represented by insoluble dietary fiber (IDF) and soluble dietary fiber (SDF) were determined.
Results—For dry or canned diets, the median reported maximum CF concentration was significantly greater than the median measured value. Measured CF concentration was significantly lower than measured TDF concentration for all diets. Median percentage of TDF (dry-matter basis) in dry and canned diets was 10.3% and 6.5%, respectively (overall range, 3.9% to 25.8%). Fiber composition in dry and canned diets differed; median percentage of TDF provided by IDF (dry-matter basis) was 83.4% in dry diets and 63.6% in canned diets.
Conclusions and Clinical Relevance—Among the evaluated diets, measured CF concentration underrepresented measured TDF concentration. Diets provided a wide range of TDF concentration, and proportions of IDF and SDF were variable. In the absence of information regarding TDF concentration, neither reported maximum nor measured CF concentration appears to be a particularly reliable indicator of fiber concentration and composition of a given canine diet.
Spatial and temporal patterns of Leptospira infection in dogs from northern California: 67 cases (2001–2010)
Authors: Hennebelle Janemarie H. , Sykes Jane E. , Carpenter Tim E. , Foley Janet 
Published: March 2013

Abstract
Objective—To conduct an epidemiological analysis of the spatial and temporal distribution of canine leptospirosis cases in northern California and detect spatial clustering in any region.
Design—Retrospective case-control study.
Animals—67 dogs with leptospirosis and 271 control dogs.
Procedures—Medical records of case and control dogs were reviewed. Spatial coordinates of home addresses of the study population were analyzed visually and statistically via a Cuzick-Edwards test and spatial, temporal, and space-time permutation scan statistics.
Results—Cases were distributed around the San Francisco Bay region as well as in the Sierra Nevada foothills near Sacramento, Calif, whereas controls were principally distributed along route I-80 between San Francisco and Sacramento, Calif. Clustering was found for the second through sixth nearest neighboring cases via the global spatial cluster test. A local spatial cluster of 30 cases was identified in San Francisco (95% confidence interval, 1.3 to 7.0), and a temporal cluster of 18 cases was identified from May 2003 through May 2004 (95% confidence interval, 1.4 to 6.5). No significant space-time cluster was identified.
Conclusions and Clinical Relevance—The use of geographic information systems provided a visual representation of the results of statistical analysis for the location and time at which leptospirosis cases occurred. This useful tool can be used to educate veterinary practitioners and the public about a potentially fatal zoonotic disease and direct vaccination strategies to help prevent disease occurrence.