bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of antimicrobial stewardship programmes and antibiotic use among children admitted to two hospitals in Sierra Leone: a cross-sectional study
Authors: Kamara Ibrahim Franklyn , Fofanah Bobson Derrick , Nuwagira Innocent , Kamara Kadijatu Nabie , Tengbe Sia Morenike , Abiri Onome , Kamara Rugiatu Z. , Lakoh Sulaiman , Farma Lynda , Kamara Abibatu Kollia , Hailu Binyam , Dove Djossaya , Squire James Sylvester , D’Almeida Selassi A. , Sheriff Bockarie , Mustapha Ayeshatu , Bawa Najima , Lagesse Hailemariam , Koroma Aminata Tigiedankay , Kanu Joseph Sam 
Published: July 2024

Abstract
Introduction
Antimicrobial resistance (AMR) is a global public health concern and irrational use of antibiotics in hospitals is a key driver of AMR. Even though it is not preventable, antimicrobial stewardship (AMS) programmes will reduce or slow it down. Research evidence from Sierra Leone has demonstrated the high use of antibiotics in hospitals, but no study has assessed hospital AMS programmes and antibiotic use specifically among children. We conducted the first-ever study to assess the AMS programmes and antibiotics use in two tertiary hospitals in Sierra Leone.

Methods
This was a hospital-based cross-sectional survey using the World Health Organization (WHO) point prevalence survey (PPS) methodology. Data was collected from the medical records of eligible patients at the Ola During Children’s Hospital (ODCH) and Makeni Regional Hospital (MRH) using the WHO PPS hospital questionnaire; and required data collection forms. The prescribed antibiotics were classified according to the WHO Access, Watch, and Reserve (AWaRe) classification. Ethics approval was obtained from the Sierra Leone Ethics and Scientific Review Committee. Statistical analysis was conducted using the SPSS version 22.

Results
Both ODCH and MRH did not have the required AMS infrastructure; policy and practice; and monitoring and feedback mechanisms to ensure rational antibiotic prescribing. Of the 150 patients included in the survey, 116 (77.3%) were admitted at ODCH and 34 (22.7%) to MRH, 77 (51.3%) were males and 73 (48.7%) were females. The mean age was 2 years (SD=3.5). The overall prevalence of antibiotic use was 84.7% (95% CI: 77.9% – 90.0%) and 77 (83.8%) of the children aged less than one year received an antibiotic. The proportion of males that received antibiotics was higher than that of females. Most (58, 47.2 %) of the patients received at least two antibiotics. The top five antibiotics prescribed were gentamycin (100, 27.4%), ceftriaxone (76, 20.3%), ampicillin (71, 19.5%), metronidazole (44, 12.1%), and cefotaxime (31, 8.5%). Community-acquired infections were the primary diagnoses for antibiotic prescription.

Conclusion
The non-existence of AMS programmes might have contributed to the high use of antibiotics at ODCH and MRH. This has the potential to increase antibiotic selection pressure and in turn the AMR burden in the country. There is need to establish hospital AMS teams and train health workers on the rational use of antibiotics.
Distributions, interactions, and dynamics of prokaryotes and phages in a hybrid biological wastewater treatment system
Authors: Wang Dou , Liu Lei , Xu Xiaoqing , Wang Chunxiao , Wang Yulin , Deng Yu , Zhang Tong 
Published: July 2024

Abstract
Background
Understanding the interactions and dynamics of microbiotas within biological wastewater treatment systems is essential for ensuring their stability and long-term sustainability. In this study, we developed a systematic framework employing multi-omics and Hi-C sequencing to extensively investigate prokaryotic and phage communities within a hybrid biofilm and activated sludge system.

Results
We uncovered distinct distribution patterns, metabolic capabilities, and activities of functional prokaryotes through the analysis of 454 reconstructed prokaryotic genomes. Additionally, we reconstructed a phage catalog comprising 18,645 viral operational taxonomic units (vOTUs) with high length and contiguity using hybrid assembly, and a distinct distribution of phages was depicted between activated sludge (AS) and biofilm. Importantly, 1340 host-phage pairs were established using Hi-C and conventional in silico methods, unveiling the host-determined phage prevalence. The majority of predicted hosts were found to be involved in various crucial metabolic processes, highlighting the potential vital roles of phages in influencing substance metabolism within this system. Moreover, auxiliary metabolic genes (AMGs) related to various categories (e.g., carbohydrate degradation, sulfur metabolism, transporter) were predicted. Subsequent activity analysis emphasized their potential ability to mediate host metabolism during infection. We also profiled the temporal dynamics of phages and their associated hosts using 13-month time-series metagenomic data, further demonstrating their tight interactions. Notably, we observed lineage-specific infection patterns, such as potentially host abundance- or phage/host ratio-driven phage population changes.

Conclusions
The insights gained from this research contribute to the growing body of knowledge surrounding interactions and dynamics of host-phage and pave the way for further exploration and potential applications in the field of microbial ecology.
The discourse on technological literacy: exploring visual representations enabled by the visual cultures of four Swedish vocational education and training programmes
Authors: Larsson Andreas , Fälton Emelie , Stolpe Karin 
Published: July 2024

AbstractIn today’s rapidly changing world, technology has profoundly impacted our daily lives, making it increasingly difficult to predict what skills will be necessary for the future. To address this challenge, 21st-century skills have been proposed as a framework for shaping future education, in which one of the skills is technological literacy. How the latter is understood, positioned, and approached is influenced by discourses produced within and among various societal practices, such as the educational system. This article provides a glimpse of the discourse on technological literacy by exploring how “technological literacy” is portrayed within the visual cultures of four Swedish upper secondary VET programs. Our results indicate that the portrayals of becoming technologically literate for VET students vary among the programmes. However, they all emphasise practical and hands-on activities, where students can be seen engaging with various tools, equipment, and objects relevant to each programme’s field of study. Teamwork and peer-to-peer learning are also portrayed as central to these programmes, simultaneously as leadership roles and the distribution of responsibilities among students are commonly featured. While the programmes’ visual portrayals reflect diversity in ethnicity and gender, traditional gender roles are still (re)produced in the pictorials.
A preclinical mice model of multiple sclerosis based on the toxin-induced double-site demyelination of callosal and cerebellar fibers
Authors: Vejar Sebastián , Pizarro Ignacio S. , Pulgar-Sepúlveda Raúl , Vicencio Sinay C. , Polit Andrés , Amador Cristian A. , del Rio Rodrigo , Varas Rodrigo , Orellana Juan A. , Ortiz Fernando C. 
Published: July 2024

Abstract
Background
Multiple sclerosis (MS) is an irreversible progressive CNS pathology characterized by the loss of myelin (i.e. demyelination). The lack of myelin is followed by a progressive neurodegeneration triggering symptoms as diverse as fatigue, motor, locomotor and sensory impairments and/or bladder, cardiac and respiratory dysfunction. Even though there are more than fourteen approved treatments for reducing MS progression, there are still no cure for the disease. Thus, MS research is a very active field and therefore we count with different experimental animal models for studying mechanisms of demyelination and myelin repair, however, we still lack a preclinical MS model assembling demyelination mechanisms with relevant clinical-like signs.

Results
Here, by inducing the simultaneous demyelination of both callosal and cerebellar white matter fibers by the double-site injection of lysolecithin (LPC), we were able to reproduce CNS demyelination, astrocyte recruitment and increases levels of proinflammatory cytokines levels along with motor, locomotor and urinary impairment, as well as cardiac and respiratory dysfunction, in the same animal model. Single site LPC-injections either in corpus callosum or cerebellum only, fails in to reproduce such a complete range of MS-like signs.

Conclusion
We here report that the double-site LPC injections treatment evoke a complex MS-like mice model. We hope that this experimental approach will help to deepen our knowledge about the mechanisms of demyelinated diseases such as MS.
Health simulation through the lens of self-determination theory — opportunities and pathways for discovery
Authors: Davies Ellen 
Published: July 2024

AbstractHealth simulation is broadly viewed as an appealing, impactful, and innovative enhancement for the education and assessment of health professions students and practitioners. We have seen exponential and global growth in programmes implementing simulation techniques and technologies. Alongside this enthusiasm and growth, the theoretical underpinnings that might guide the efficacy of the field have not always been considered. Many of the principles that guide simulation design, development and practice have been intuited through practical trial and error. In considering how to retrofit theory to practice, we have at our disposal existing theories that may assist with building our practice, expertise, identity as a community of practice, authority and legitimacy as a field. Self-determination theory (SDT) is an established and evolving theory that examines the quality of motivation and human behaviours. It has been applied to a variety of contexts and provides evidence that may support and enhance the practice of health simulation. In this paper, SDT is outlined, and avenues for examining the fit of theory to practice are suggested. Promising links exist between SDT and health simulation. Opportunities and new pathways of discovery await.
The heritagization of cultural politics: anthropological research on Chinese cultural heritage
Authors: Zhou Xing , Huang Jie 
Published: July 2024

AbstractSince the beginning of the twenty-first century, in alignment with globalization and the megatrend within the international socio-cultural sphere, China has initiated a large-scale “intangible cultural heritage (ICH) protection movement” through its proactive leadership and management of cultural heritage administration. As a significant component of Chinese cultural politics, this movement has profoundly influenced the cultural lives of Chinese society and its citizens and is poised to continue doing so. Some Chinese anthropologists have also participated in and significantly contributed to this movement in various ways. This paper comprehensively and meticulously examines these fundamental ways. Building upon this analysis, this paper further provides an overview and assessment of the research outcomes of anthropological research on Chinese cultural heritage, both domestically and internationally. The author posits that the paramount contribution of anthropologists, both domestically and internationally, to Chinese cultural heritages and the related protection endeavors lies in their academic research outcomes being predominantly based on their respective fieldwork practices. These outcomes, shaped and realized through on-the-ground fieldwork, hold exceptional value for cultural heritage administration and national cultural policies, as they effectively provide cultural criticism and dissenting perspectives.
Calibration of flow differential pressure coefficients on pump-turbine by thermodynamic method
Authors: Zhang Wenkui , Xu Hongyi , Liu Wei , Hu Xinwen , Liu Juan 
Published: July 2024

AbstractOn-line monitoring of flow rate is rather important to evaluate the water consumption of the pump-turbine and the overall efficiency of the pumped storage station. Flow rate can be worked out based on the flow differential pressure measured in the flow passage and coefficients determined on model test. But the coefficients must be calibrated to obtain the reliable flow rate. As thermodynamic method is a common way to measure the discharge of prototype pump-turbine, site test is carried out on a vertical pump-turbine with 600 m rated head to calibrated the coefficients in this paper. Measuring devices are designed to satisfy both the turbine and pump operating conditions, and test results of flow rate at both turbine and pump operating conditions are presented and show that the calibrated coefficients are different to the predicted values. The calibrated results indicate the importance of site calibration on prototype turbine to ensure the monitoring validity on real-time flow rate.
Coexisting with wildfire: strengthening collective capacity by changing the status quo
Authors: Eriksen Christine 
Published: July 2024

AbstractThis article is the fuller written version of the invited closing plenary given by the author at the 10th International Fire Ecology and Management Congress. The article provides a consideration of our capacity to cope, care, and coexist in a fiery world from a social and structural point of view. It focuses on privilege as the root cause of a long and troublesome history within the wildfire profession of not valuing all generational knowledge equally, not treating all cultures with the same respect, not embracing diversity and inclusion, and not affording the same status to all disciplines and voices. The article argues that we can strengthen our collective capacity to coexist with wildfire by embracing local and indigenous fire stewardship practices, by enabling workforce diversity and inclusive leadership culture, and by providing sustainable working conditions for wildland firefighters. To do so requires individual and collective noticing of what is wrong, and everyday action steps towards equity.
Seasonal life cycle of Pintomyia longiflocosa (Diptera: Psychodidae) under semi-controlled conditions in the Huila state, Colombia
Authors: Vargas Durango Jennifer Alejandra , Mestre Carillo Gelys Igreth , Santamaria Herreño Erika , Beltran Ruiz Miguel Angel , Zapata Lesmes Angela Cristina , Muñoz Ortiz Astrid Geovana , Escobar Castro Jesús Eduardo 
Published: July 2024

Background
Pintomyia longiflocosa is considered a vector of etiological agents of cutaneous leishmaniasis. The objective of this research was to establish the parameters of the life cycle of Pi. longiflocosa in semi-controlled conditions in a rural area of the Campoalegre municipality, Huila, Colombia.

Methodology/principal findings
The life cycle of individuals of Pi. longiflocosa, obtained from two cohorts of collected, fed, and individualized females, was monitored during two different times of the year (between February and August 2020 and between July 2020 and January 2021, respectively). Determining parameters associated with the fertility and fecundity, time and attributes of development and survival, and its association with abiotic variables. The average duration of Cycle 1 (C1) and Cycle 2 (C2) was 134.9 and 148.78 days, respectively. The gonotrophic cycle of parental females presented significant differences (p-value <0.05) between C1 and C2 (8.47 and 11.42 days) as well as between fecundity and fertility parameters. The number of days it takes the development of the immature stages between the two cycles studied, also showed significant differences in the larvae II (15.21 and 22.23), larvae III (11.93 and 17.56), and pupae (24.48 and 22.9) stages. During C1, the survival rate was higher and consistent with the productivity of adult individuals (F1), compared to C2. Fecundity and fertility values were significantly higher in C2. Finally, a significant correlation between the number of individuals and temperature was evidenced in C1 while, for C2, there was a negative correlation with precipitation.

Conclusions/significance
Significant differences were found in several biological and reproductive parameters between the two cycles monitored. The parameters of the life cycle of Pi. longiflocosa in its natural habitat would be influenced by environmental factors related to the annual seasonality in the sub-Andean rural area, conditioning the temporal distribution of this species and, consequently, the possible transmission of causative agents of cutaneous leishmaniasis.
Impact of ivermectin and vector control on onchocerciasis transmission in Togo: Assessing the empirical evidence on trends in infection and entomological indicators
Authors: Vinkeles Melchers Natalie V. S. , Agoro Sibabi , Togbey Kwamy , Padjoudoum Koffi , Telou Ibrahim Gado , Karabou Potchoziou , Djatho Touka , Datagni Michel , Dorkenoo Ameyo Monique , Kassankogno Yao , Bronzan Rachel , Stolk Wilma A. 
Published: July 2024

Background
The World Health Organization’s 2021–2030 Road Map for Neglected Tropical Diseases boosted global commitment towards the elimination of onchocerciasis, but the timeline to elimination will vary strongly between countries in Africa. To assess progress towards elimination in the Republic of Togo, we reviewed the history of control and time trends in infection.

Methodology/principal findings
We collated all available programmatic, entomological, and epidemiological data since the initiation of the Onchocerciasis Control Programme (OCP) in Togo through different data sources. We then visualised data trends over time, to assess the impact of interventions on infection and transmission levels. Vector control was initiated by OCP from 1977 (northern and central parts of Togo) or 1988 (southern regions) up to 2002 (most areas) or 2007 (“special intervention zones” [SIZ], parts of Northern and Central Togo). Between 1988 and 1991, Togo initiated ivermectin mass drug administration (MDA) in eligible communities. The impact of vector control was high in most river basins, resulting in low annual biting rates and annual transmission potential declining to very low levels; the impact was lower in river basins designated as SIZ. Repeated, longitudinal ivermectin mass treatments have overall strongly reduced onchocerciasis transmission in Togo. Epidemiological surveys performed between 2014 and 2017 showed that the prevalence of skin microfilariae (mf) and anti-OV16 IgG4 antibodies had declined to low levels in several districts of the Centrale, Plateaux, and Maritime region. Yet, relatively high mf prevalences (between 5.0% and 32.7%) were still found in other districts during the same period, particularly along the Kéran, Mô and Ôti river basins (SIZ areas).

Conclusions/significance
Trends in infection prevalence and intensity show that onchocerciasis levels have dropped greatly over time in most areas. This demonstrates the large impact of long-term and wide-scale interventions, and suggest that several districts of Togo are approaching elimination.
Predicting postoperative visual acuity in epiretinal membrane patients and visualization of the contribution of explanatory variables in a machine learning model
Authors: Irie-Ota Akiko , Matsui Yoshitsugu , Imai Koki , Mase Yoko , Konno Keiichiro , Sasaki Taku , Chujo Shinichiro , Matsubara Hisashi , Kawanaka Hiroharu , Kondo Mineo 
Published: July 2024

Background
The purpose of this study was to develop a model that can predict the postoperative visual acuity in eyes that had undergone vitrectomy for an epiretinal membrane (ERM). The Light Gradient Boosting Machine (LightGBM) was used to evaluate the accuracy of the prediction and the contribution of the explanatory variables. Two models were designed to predict the postoperative visual acuity in 67 ERM patients. Model 1 used the age, sex, affected eye, axial length, preoperative visual acuity, Govetto’s classification stage, and OCT-derived vector information as features to predict the visual acuity at 1, 3, and 6 months postoperatively. Model 2 incorporated the early postoperative visual acuity as an additional variable to predict the visual acuity at 3, and 6 months postoperatively. LightGBM with 100 iterations of 5-fold cross-validation was used to tune the hyperparameters and train the model. This involved addressing multicollinearity and selecting the explanatory variables. The generalized performance of these models was evaluated using the root mean squared error (RMSE) in a 5-fold cross-validation, and the contributions of the explanatory variables were visualized using the average Shapley Additive exPlanations (SHAP) values.

Results
The RMSEs for the predicted visual acuity of Model 1 were 0.14 ± 0.02 logMAR units at 1 month, 0.12 ± 0.03 logMAR units at 3 months, and 0.13 ± 0.04 logMAR units at 6 months. High SHAP values were observed for the preoperative visual acuity and the ectopic inner foveal layer (EIFL) area with significant and positive correlations across all models. Model 2 that incorporated the postoperative visual acuity was used to predict the visual acuity at 3 and 6 months, and it had superior accuracy with RMSEs of 0.10 ± 0.02 logMAR units at 3 months and 0.10 ± 0.04 logMAR units at 6 months. High SHAP values were observed for the postoperative visual acuity in Model 2.

Conclusion
Predicting the postoperative visual acuity in ERM patients is possible using the preoperative clinical data and OCT images with LightGBM. The contribution of the explanatory variables can be visualized using the SHAP values, and the accuracy of the prediction models improved when the postoperative visual acuity is included as an explanatory variable. Our data-driven machine learning models reveal that preoperative visual acuity and the size of the EIFL significantly influence postoperative visual acuity. Early intervention may be crucial for achieving favorable visual outcomes in eyes with an ERM.