bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emotion Coupling and Regulation in Anorexia Nervosa
Authors: Fox John R. E. , Smithson Emily , Baillie Sarah , Ferreira Nuno , Mayr Ingrid , Power Michael J. 
Published: November 2012

ObjectiveThe present study sought to investigate emotion regulation strategies in people with anorexia nervosa (AN) and whether the theoretical concept of ‘emotion coupling’ between anger and disgust could help to explain some of the specific eating disorder symptomatology in people with AN.MethodThis ‘emotion coupling’ hypothesis was tested using a mood induction procedure within laboratory conditions, where individuals with AN (n = 22) were matched with control participants (n = 19). Participants completed a bank of different measures prior to the study, and these included measures of eating pathology, core beliefs about the self and others, and emotion regulation strategies. Within the experimental part of this study, anger, disgust and body size estimation were measured prior to and after an anger induction procedure (i.e., a repeated measures design).ResultsPeople with AN demonstrated a significantly more internal‐dysfunctional way to regulate their emotional states, when compared with matched controls. Within the ‘emotional coupling’ part of the study, participants showed a significant increase in levels of disgust and body size estimation following an anger induction when compared with matched controls.DiscussionThe significance of these results was considered in the light of the new Schematic, Propositional, Analogical and Associative Representation Systems in eating disorders model. Copyright © 2012 John Wiley & Sons, Ltd.Key Practitioner MessageEmotions and emotion processing are problematic for people with anorexia nervosa.Overestimation in body size for people with anorexia nervosa may be due to the coupling of anger and disgust.Clinicians need to consider the importance of automatic cognitive routes of emotion elicitation that may maintain certain eating disorder symptoms, such as poor body image.
Type 2 diabetes patients educated by other patients perform at least as well as patients trained by professionals
Authors: Gagliardino Juan José , Arrechea Viviana , Assad Daniel , Gagliardino Gabriel G. , González Lorena , Lucero Soledad , Rizzuti Liliana , Zufriategui Zulma , Clark Charles 
Published: November 2012

AbstractBackgroundDiabetes education can improve the quality of care of people with diabetes, but many organizations are not equipped to manage its implementation. Involving people with diabetes in the education process can overcome the problem. Thus, we compared clinical, metabolic and psychological outcomes in people with type 2 diabetes 1 year after attending a structured diabetes education programme implemented by professional educators versus the same programme implemented by trained peers with diabetes that also provided ongoing peer support.MethodsPeople with type 2 diabetes (25–75 years) were randomly assigned to attend a 4‐week structured diabetes education course delivered by professional educators (control) or previously trained peers (peer). Peers also received continuing psychological support, including examples on how to apply diabetes knowledge in daily life via weekly peer cellular phone calls and bimonthly face‐to‐face interviews in small groups (ten patients), using a structured questionnaire related to the patient's clinical, metabolic and psychological progress. Identical outcome data from both groups were used for follow‐up.ResultsBoth groups had a comparable positive effect on clinical, metabolic and psychological indicators immediately following the programme. Over the following year, peer‐educated subjects had lower A1C and systolic blood pressure and showed higher adherence to physical activity and better control of hypoglycaemic episodes.ConclusionThe non‐inferiority of the peer outcomes and the mentioned improvements in this group suggest that volunteer trained peer educators and ongoing support can be successful. This approach provides an effective alternative method of education, especially in areas with limited availability of professionals and economic resources. Copyright © 2012 John Wiley & Sons, Ltd.
Developmental expression of Drosophila Wiskott‐Aldrich Syndrome family proteins
Authors: Rodriguez‐Mesa Evelyn , Abreu‐Blanco Maria Teresa , Rosales‐Nieves Alicia E. , Parkhurst Susan M. 
Published: November 2012

AbstractCOVER PHOTOGRAPH: High magnification confocal image of a stage 8 Drosophila egg chamber immuno‐stained with antibodies recognizing Whamy (shown in green) and the golgi protein Lava lamp (shown in red). DNA shown in blue. From Thomason et al., Developmental Dynamics 241:608–626, 2012.
Modification of Tetranuclear Zirconium‐Substituted Polyoxometalates – Syntheses, Structures, and Peroxidase‐Like Catalytic Activities
Authors: Li Dan , Han Huayan , Wang Yonghui , Wang Xin , Li Yangguang , Wang Enbo 
Published: November 2012

AbstractThe introduction of hydrogen peroxide, dl‐mandelic acid (H2Mal), and acetate buffer into a reaction system containing zirconium(IV) ions and various trivacant polyoxometalates (POMs) led to the isolation of a series of new tetranuclear zirconium‐substituted POMs modified by different O‐donor ligands: Na4(NH4)14[Zr4(μ3‐O)2(μ‐O2)2(OAc)2(P2W16O59)2]·51H2O (1), (NH4)16[Zr4(μ3‐O)2(Mal)2(H2O)2(P2W16O59)2]·14H2O (2), and Na7(NH4)5[{Zr4(OH)6(OAc)2}(SiW10O37)2]·20H2O(3) (3). All three compounds were characterized by elemental analyses, IR spectra, thermogravimetric (TG) analysis and single‐crystal X‐ray diffraction structural analyses. Compounds 1 and 2 possess similar sandwich‐type polyoxoanion structures built from a tetranuclear Zr cluster and two [P2W16O59]12– building blocks, but they differ in the modifying ligands on the {Zr4} clusters, which are peroxido and mandelato for 1 and 2, respectively. The polyoxoanion of 3 contains a different tetranuclear Zr cluster, which is further sandwiched by two [α‐SiW10O37]10– moieties and decorated by two acetato ligands. The catalytic activities of 1–3 were evaluated on the basis of a catalytic oxidation reaction of the peroxidase substrate 3,3′,5,5′‐tetramethylbenzidine (TMB) by H2O2. Compound 1 exhibits the best catalytic activity, which suggests a new peroxidase‐like catalyst for the colorimetric detection of H2O2 with a linear detection range from 100 to 1000 μM (r = 0.997) and a detection limit of 100 μM.
Modified SWCNTs for Reagentless Glucose Biosensor: Electrochemical and Mathematical Characterization
Authors: Razumiene J. , Gureviciene V. , Sakinyte I. , Barkauskas J. , Petrauskas K. , Baronas R. 
Published: November 2012

AbstractAn original concept in technology of biosensitive electrodes based on single‐wall carbon nanotubes (SWCNT) is proposed. According to a newly proposed technique the electrode surface consisting of SWCNT’s embedded in a porous polycarbonate film was preoxidized enzymatically using laccase from Basidiomycete Lac. An efficient electron transfer from the active centre of PQQ‐dependent glucose dehydrogenase (s‐PQQ‐GDH) to the SWCNT electrode surface allowed the application of the s‐PQQ‐GDH as recognition element for the reagentless biosensor design. The residual activity of the biosensor was found to be 40 % after one month of operational working. A mathematical model of the biosensor has been developed and an admissible agreement between the simulation results and the experimental data was obtained. The model appears to be promising for optimization of amperometric analysis.
Selective Curcuminoid Separation and Detection via Nickel Complexation and Adsorptive Stripping Voltammetry
Authors: Wray David M. , Batchelor‐McAuley Christopher , Compton Richard G. 
Published: November 2012

AbstractCurcumin is a widely studied biologically active redox molecule. Although sensitive, electrochemical methods are commonly hindered by their lack of specificity. This article demonstrates how curcumin may be readily separated from other structurally and electrochemically similar redox moieties. This separation is achieved through exploitation of the molecules diketone functionality providing a route for its selective chelation to nickel(II). Recovery of the curcumin from the nickel complex is achieved simply and quantitatively through acidification of the precipitate. Adsorptive stripping voltammetry at a carbon screen‐printed electrode (SPE) is utilised both as a method for evidencing the effectiveness of the separation technique but further as a procedure for the analytical quantification of the curcumin concentration.
Application of time preconditioning and high‐order compact discretization method for low Mach number flows
Authors: Tyliszczak A. , Deconinck H. 
Published: November 2012

SUMMARYThe paper describes a combination of a preconditioning method with a high‐order compact discretization scheme for the purpose of solving the compressible Navier–Stokes equations in moderate and low Mach number regimes. When combined with properly modified characteristic boundary conditions, the proposed approach is very efficient from the point of view of convergence acceleration and accuracy of the results. The computations were performed in typical benchmark cases including the Burggraf flow for which an analytical solution exists, the flow over a backward facing step, and also the flow in 2D and 3D shear‐driven cavities. Depending on the test case, the results were obtained for the Mach number in the range M = 0.001 − 0.5 and the Reynolds number Re = 1 − 1000. Copyright © 2012 John Wiley & Sons, Ltd.
Describing perceived stigma against Alzheimer's disease in a general population in France: the STIG‐MA survey
Authors: Piver Leslie Cartz , Nubukpo Philippe , Faure Angélique , Dumoitier Nathalie , Couratier Philippe , Clément Jean‐Pierre 
Published: November 2012

BackgroundAlzheimer's disease (AD) causes progressive loss of memory and disability, especially in older people. As worldwide population grows older, AD is responsible for an important social and economical burden in many nations. People suffering from AD may experience health‐related stigma that influences their attitudes towards seeking assistance. The STIG‐MA survey describes perceived stigma against AD in a French population.MethodsThe STIG‐MA questionnaire was completed anonymously by people attending an awareness campaign about AD in Creuse, France, in September 2010. Participants answered 10 questions about how they would feel or react if they had AD. Stigma scores were compared by age, activity, and interest in AD.ResultsThirty‐three percent of people attending the campaign filled out the survey. Most were women (85%) younger than 50 years (59%); 10% were older people (older than 75 years). Twenty‐one percent worked in health or social fields. Interest in AD was professional (48%), related to family (41%), or personal (11%). Professionals in health fields expressed the highest levels of stigma (p = 0.02). Low stigma was most frequent in older people (p = 0.05). Type of interest did not influence stigma. Shame, loss of self‐esteem, and fear of exclusion were expressed the most.ConclusionThe STIG‐MA survey confirms that AD is a stigmatizing condition in France. The difference between perceived stigma of older people, those most exposed to AD, and that of health professionals may influence attitudes towards screening and care. Further studies of perceived stigma in these populations are necessary to adapt intervention strategies. Copyright © 2012 John Wiley & Sons, Ltd.
Quantifying sources of increasing zinc from acid rock drainage in an alpine catchment under a changing hydrologic regime
Authors: Crouch Caitlin M. , McKnight Diane M. , Todd Andrew S. 
Published: November 2012

AbstractA major water quality concern in the Rocky Mountains is acid rock drainage (ARD), which causes acidic conditions and high metal concentrations in streams. The 30‐year water quality record for the upper Snake River in Colorado, USA, shows that summer low‐flow zinc concentrations have increased four‐ to sixfold concurrently with increases in mean annual and summer temperatures and a two‐ to three‐week advancement in spring snowmelt. We found that the main source of zinc and other metal loads to the upper Snake River was a tributary draining an alpine area rich in disseminated pyrite. By conducting a tracer experiment in this tributary of interest, we demonstrated that 30% of the trace metal loading entered in an upper steep, rocky reach where the tributary of interest is fed by an alpine spring. Another increase in flow and metal loading occurred where the tributary of interest flows through a gently sloped wetland area. Analysis of the tracer experiment indicated a significant increase in hyporheic exchange along this wetland reach, from which metals appear to be mobilized. Several potential explanations are presented that might explain this phenomenon: decreasing pH in the tributary of interest may result in mobilization of metals from the wetland and hyporheic zone; the geochemistry of groundwater inflows to the wetland may be changing; and, wetland soils may be drying out with longer, warmer summers. This study illustrates how changes in hydrologic regime may cause changes in biogeochemical processes that exacerbate the danger to aquatic ecosystems associated with ARD. Copyright © 2012 John Wiley & Sons, Ltd.
Activated leukocyte cell adhesion molecule soluble form: a potential biomarker of epithelial ovarian cancer is increased in type II tumors
Authors: Carbotti Grazia , Orengo Anna Maria , Mezzanzanica Delia , Bagnoli Marina , Brizzolara Antonella , Emionite Laura , Puppo Andrea , Centurioni Maria Grazia , Bruzzone Milena , Marroni Paola , Rossello Armando , Canevari Silvana , Ferrini Silvano , Fabbi Marina 
Published: November 2012

AbstractActivated leukocyte cell adhesion molecule (ALCAM) is involved in cell–cell interactions in cancer. Shedding of its ectodomain by the metalloprotease ADAM17/TACE generates a soluble form (sALCAM). Here, we show that serum sALCAM levels were significantly higher in epithelial ovarian cancer (EOC) (p < 0.005) than in controls. The performance of sALCAM as classifier, tested by receiver operating characteristic curve, resulted in an area under the curve (AUC) of 0.8067. Serum sALCAM levels showed direct correlation with Carbohydrate Antigen‐125 (CA125/MUC16). Moreover, significantly higher levels were found in type II tumors, even in stage I/II, suggesting that elevated sALCAM is an early feature of aggressive EOC. In addition, sALCAM levels were higher in ascites than in sera, suggesting local processing of ALCAM in the peritoneal cavity. In immunodeficient mice, intraperitoneally implanted with a human EOC cell line, human sALCAM progressively increased in serum and was even higher in the ascites. The biochemical characterization of the sALCAM in EOC sera and ascites, showed two predominant forms of approximately 95 and 65 kDa but no EOC‐specific isoform. In addition, full‐length transmembrane ALCAM but no soluble form was detected in tumor‐derived exosomes found in ascites. Finally, in vitro invasion assays showed that inhibition of ADAM17/TACE activity decreased EOC invasive properties, while opposite effects were mediated by a sALCAM‐Fc chimera and by an antibody interfering with ALCAM/ALCAM interactions. Altogether these data suggest that sALCAM is a marker of EOC, which correlates with more aggressive type II tumors, and that ADAM17/TACE activity and sALCAM itself mediate enhanced invasiveness.
pH responsive PEGylation through metal affinity for gene delivery mediated by histidine‐grafted polyethylenimine
Authors: Tseng Wen‐Chi , Su Ling‐Yu , Fang Tsuei‐Yun 
Published: November 2012

AbstractPolyethylene glycol (PEG) has been used to enhance the stability of a gene delivery system. The most commonly used approach is to add the PEG molecule by way of chemical conjugation. In this study, we prepared PEG‐bearing nitrilotriacetic acid (ntaPEG) followed by chelation with either nickel or zinc ions. Polyethylenimine was grafted with histidine (hisPEI) and used as a primary gene carrier to form complexes with DNA. PEGylation was performed by incubating the complexes with chelated ntaPEG. It was noted that the coating of the chelated ntaPEG could provide a shielding effect against aggregation induced by bovine serum albumin and DNA release induced by heparin displacement, respectively. The coating was also found to improve cellular viability and maintain the transfection efficiency at a moderate level. The coated ntaPEG could dissociate from the complexes in an acidic condition of pH 4, suggesting that dePEGylation might occur in some acidic intracellular organelles, such as endosomes. This simple and effective PEGylation approach could be extended to other delivery systems to enhance the stability and to facilitate the dePEGylation process. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
CD44 enhances tumor formation and lung metastasis in experimental osteosarcoma and is an additional predictor for poor patient outcome
Authors: Gvozdenovic Ana , Arlt Matthias JE , Campanile Carmen , Brennecke Patrick , Husmann Knut , Li Yufei , Born Walter , Muff Roman , Fuchs Bruno 
Published: November 2012

Abstract
Formation of metastases in the lungs is the major cause of death in patients suffering from osteosarcoma (OS). Metastases at presentation and poor response to preoperative chemotherapy are strong predictors for poor patient outcome. The elucidation of molecular markers that promote metastasis formation and/or chemoresistance is therefore of importance. CD44 is a plasma membrane glycoprotein that binds to the extracellular matrix component hyaluronan (HA) and has been shown to be involved in metastasis formation in a variety of other tumors. Here we investigated the role of CD44 expression on OS tumor formation and metastasis. High CD44 expression, evaluated with a tissue microarray including samples from 53 OS patients and stained with a pan-CD44 antibody (Hermes3), showed a tendency (p &lt; 0.08) to shortened overall survival. However, nonresponders and patients with lung metastases and high CD44 expression had significantly poorer prognosis than patients with low CD44 expression. Overexpression of the standard CD44 isoform (CD44s) and its HA-binding defective mutant R41A in osteoblastic SaOS-2 cells resulted in HA-independent higher migration rates and increased chemoresistance, partially dependent on HA. In an orthotopic mouse model of OS, overexpression of CD44s in SaOS-2 cells resulted in an HA-dependent increased primary tumor formation and increased numbers of micrometastases and macrometastases in the lungs. In conclusion, although CD44 failed to be an independent predictor for patient outcome in this limited cohort of OS patients, increased CD44 expression was associated with even worse survival in patients with chemoresistance and with lung metastases. CD44-associated chemoresistance was also observed in vitro, and increased formation of lung metastases was found in vivo in SCID mice. © 2013 American Society for Bone and Mineral Research.
Genes of Innate Immunity and the Biological Response to Inhaled Ozone
Authors: Li Zhuowei , Tighe Robert M. , Feng Feifei , Ledford Julie G. , Hollingsworth John W. 
Published: November 2012

ABSTRACTAmbient ozone has a significant impact on human health. We have made considerable progress in understanding the fundamental mechanisms that regulate the biological response to ozone. It is increasingly clear that genes of innate immunity play a central role in both infectious and noninfectious lung disease. The biological response to ambient ozone provides a clinically relevant environmental exposure that allows us to better understand the role of innate immunity in noninfectious airways disease. In this brief review, we focus on (1) specific cell types in the lung modified by ozone, (2) ozone and oxidative stress, (3) the relationship between genes of innate immunity and ozone, (4) the role of extracellular matrix in reactive airways disease, and (5) the effect of ozone on the adaptive immune system. We summarize recent advances in understanding the mechanisms that ozone contributes to environmental airways disease. © 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:3‐16, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21453
Optimization of Fluorescence Assay of Cellular Manganese Status for High Throughput Screening
Authors: Kumar Kevin K. , Aboud Asad A. , Patel Devin K. , Aschner Michael , Bowman Aaron B. 
Published: November 2012

ABSTRACTThe advent of high throughput screening (HTS) technology permits identification of compounds that influence various cellular phenotypes. However, screening for small molecule chemical modifiers of neurotoxicants has been limited by the scalability of existing phenotyping assays. Furthermore, the adaptation of existing cellular assays to HTS format requires substantial modification of experimental parameters and analysis methodology to meet the necessary statistical requirements. Here we describe the successful optimization of the Cellular Fura‐2 Manganese Extraction Assay (CFMEA) for HTS. By optimizing cellular density, manganese (Mn) exposure conditions, and extraction parameters, the sensitivity and dynamic range of the fura‐2 Mn response was enhanced to permit detection of positive and negative modulators of cellular manganese status. Finally, we quantify and report strategies to control sources of intra‐ and interplate variability by batch level and plate‐geometric level analysis. Our goal is to enable HTS with the CFMEA to identify novel modulators of Mn transport. © 2012 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:42‐49, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21457
RUNX2 in mammary gland development and breast cancer
Authors: Ferrari Nicola , McDonald Laura , Morris Joanna S. , Cameron Ewan R. , Blyth Karen 
Published: November 2012

AbstractRunx2 is best known as an essential factor in osteoblast differentiation and bone development but, like many other transcription factors involved in development, is known to operate over a much wider tissue range. Our understanding of these other aspects of Runx2 function is still at a relatively early stage and the importance of its role in cell fate decisions and lineage maintenance in non‐osseous tissues is only beginning to emerge. One such tissue is the mammary gland, where Runx2 is known to be expressed and participate in the regulation of mammary specific genes. Furthermore, differential and temporal expression of this gene is observed during mammary epithelial differentiation in vivo, strongly indicative of an important functional role. Although the precise nature of that role remains elusive, preliminary evidence hints at possible involvement in the regulation of mammary stem and/or progenitor cells. As with many genes important in regulating cell fate, RUNX2 has also been linked to metastatic cancer where in some established breast cell lines, retention of expression is associated with a more invasive phenotype. More recently, expression analysis has been extended to primary breast cancers where high levels of RUNX2 align with a specific subtype of the disease. That RUNX2 expression correlates with the so called “Triple Negative” subtype is particularly interesting given the known cross talk between Runx2 and estrogen receptor signaling pathways. This review summaries our current understanding of Runx2 in mammary gland development and cancer, and postulates a role that may link both these processes. J. Cell. Physiol. 228: 1137–1142, 2013. © 2012 Wiley Periodicals, Inc.