bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of human vocal fold fibroblasts derived from chronic scar
Authors: Jetté Marie E. , Hayer Supriya D. , Thibeault Susan L. 
Published: February 2013

AbstractObjectives/Hypothesis:In vitro modeling of cell‐matrix interactions that occur during human vocal fold scarring is uncommon, as primary human vocal fold scar fibroblast cell lines are difficult to acquire. The purpose of this study was to characterize morphologic features, growth kinetics, contractile properties, α‐smooth muscle actin (α‐SMA) protein expression and gene expression profile of human vocal fold fibroblasts derived from scar (sVFF) relative to normal vocal fold fibroblasts (nVFF).Study Design:In vitro.Methods:We successfully cultured human vocal fold fibroblasts from tissue explants of scarred vocal folds from a 56‐year‐old female and compared these to normal fibroblasts from a 59‐year‐old female. Growth and proliferation were assessed by daily cell counts, and morphology was compared at 60% confluence for 5 days. Gel contraction assays were evaluated after seeding cells within a collagen matrix. α‐SMA was measured using western blotting and immunocytochemistry (ICC). Quantitative reverse‐transcriptase polymerase chain reaction (qRT‐PCR) was used to assess differential extracellular matrix gene expression between the two cell types.Results:sVFF were morphologically indistinguishable from nVFF. sVFF maintained significantly lower proliferation rates relative to nVFF on days 3 to 6 (day 3: P = .0138; days 4, 5, and 6: P < .0001). There were no significant differences in contractile properties between the two cell types at any time point (0 hours: P = .70, 24 hours: P = .79, 48 hours: P = .58). ICC and western blot analyses revealed increased expression of α‐SMA in sVFF as compared with nVFF at passages 4 and 5, but not at passage 6 (passage 4: P = .006, passage 5: P = .0015, passage 6: P = .8860). Analysis of 84 extracellular matrix genes using qRT‐PCR revealed differential expression of 15 genes (P < .01).Conclusions:nVFF and sVFF displayed differences in proliferation rates, α‐SMA expression, and gene expression, whereas no differences were observed in contractile properties or morphology. Further investigation with a larger sample size is necessary to confirm these findings. Laryngoscope, 2013
Outcomes in head and neck oncologic surgery at academic medical centers in the united states
Authors: Jalisi Scharukh , Bearelly Shethal , Abdillahi Abdirahman , Truong Minh Tam 
Published: February 2013

AbstractObjectives/Hypothesis:To evaluate the impact of case volume and other variables on outcomes after head and neck oncologic surgery was performed at academic medical centers in the United States.Study Design:Cross Sectional Ecological Study.Methods:The University HealthSystems Consortium (UHC) database was analyzed for discharge data on all patients who underwent surgery for head and neck cancers (excluding thyroid and skin cancer) at full‐ member academic medical centers between quarter 4 of 2006 and quarter 4 of 2009. Multivariate and linear regression analyses and chi‐square tests were applied to evaluate significant associations between hospital surgical volume and other independent variables, and to evaluate the risk of mortality, mortality index, complications, length of stay (LOS), LOS index, cost, and cost index.Results:Of 22,357 surgical cases, 11,573 met our inclusion criteria. The only outcome that was statistically significant based on volume was a lower complication rate in high volume hospitals (P = 0.0486) as compared to low volume hospitals. All Payer Refined–Diagnosis Related Group defined major severity of illness was the only independent variable significantly associated with higher complication rates, observed LOS, and observed cost (P <0.0001, P = 0.0139, and P = 0.0092, respectively). Management of male patients and black patients resulted in a lower cost index (P = 0.0472) and a higher complication rate (P = 0.0297), respectively. Patients with private insurance had lower complication rates, observed LOS, and observed cost (P = 0.0401, P = 0.0001, and P = 0.0187, respectively).Conclusions:After controlling for other factors, academic medical centers with a higher cumulative case volume have lower rates of complications. Laryngoscope, 2013
Numerical modelling of heavy rainfall event over Madeira Island in Portugal: sensitivity to different micro physical processes
Authors: Dasari Hari Prasad , Salgado Rui 
Published: February 2013

AbstractMadeira Island, Portugal, experienced intense heavy rainfall on 20 February 2010 which caused loss of more than 40 lives and great damage. This is recorded as one of the major flash flood events during the past three decades. This heavy rainfall episode is simulated using the Advanced Research Weather Research Forecasting (ARW) model at a cloud resolving grid resolution of 1 km. A nested two‐way interactive four‐domain configuration centred on the island and forced laterally by the 6 hourly NCEP FNL 1° data are used. Four experiments with different cloud microphysical parameterizations are performed to examine the model sensitivity to cloud microphysics on heavy rainfall simulations. Analysis of the synoptic meteorological conditions indicated that the extreme rainfall resulted due to the effect of regional orography on the prevailing large scale flow with conditionally unstable moist air. Results indicate that the model is able to reproduce the heavy rainfall with good agreement. The experiment with the Ferrier scheme produced more rainfall than the other three experiments, as well as overestimating the quantity of rainfall. Analysis of simulated cloud microphysical properties indicated that the model‐produced heavy rainfall is sensitive to different cloud microphysics schemes. Model quantitative rainfall comparisons at different stations indicate that the Lin and Thompson schemes produced a more realistic simulation of heavy rainfall with relatively better correlation and higher threat score than the other two schemes. The associated dynamic and thermodynamic parameters are well produced in these two simulations. Copyright © 2013 Royal Meteorological Society
SVM Based Descriptor Selection and Classification of Neurodegenerative Disease Drugs for Pharmacological Modeling
Authors: Shahid Mohammad , Shahzad Cheema Muhammad , Klenner Alexander , Younesi Erfan , Hofmann‐Apitius Martin 
Published: February 2013

AbstractSystems pharmacological modeling of drug mode of action for the next generation of multitarget drugs may open new routes for drug design and discovery. Computational methods are widely used in this context amongst which support vector machines (SVM) have proven successful in addressing the challenge of classifying drugs with similar features. We have applied a variety of such SVM‐based approaches, namely SVM‐based recursive feature elimination (SVM‐RFE). We use the approach to predict the pharmacological properties of drugs widely used against complex neurodegenerative disorders (NDD) and to build an in‐silico computational model for the binary classification of NDD drugs from other drugs. Application of an SVM‐RFE model to a set of drugs successfully classified NDD drugs from non‐NDD drugs and resulted in overall accuracy of ∼80 % with 10 fold cross validation using 40 top ranked molecular descriptors selected out of total 314 descriptors. Moreover, SVM‐RFE method outperformed linear discriminant analysis (LDA) based feature selection and classification. The model reduced the multidimensional descriptors space of drugs dramatically and predicted NDD drugs with high accuracy, while avoiding over fitting. Based on these results, NDD‐specific focused libraries of drug‐like compounds can be designed and existing NDD‐specific drugs can be characterized by a well‐characterized set of molecular descriptors.
Activity Landscapes, Information Theory, and Structure – Activity Relationships
Authors: Iyer Preeti , Stumpfe Dagmar , Vogt Martin , Bajorath J. , Maggiora G. M. 
Published: February 2013

AbstractActivity landscapes provide a comprehensive description of structure‐activity relationships (SARs). An information theoretic assessment of their features, namely, activity cliffs, similarity cliffs, smooth‐SAR, and featureless regions, is presented based on the probability of occurrence of these features. It is shown that activity cliffs provide highly informative SARs compared to smooth‐SAR regions, although the latter are the basis for most QSAR studies. This follows since small structural changes in the former are coupled with relatively large changes in activity, thus pinpointing specific structural features associated with the changes in activity. In contrast, Smooth‐SAR regions are typically associated with relatively small changes in both structure and activity. Surprisingly, similarity cliffs, which occur when both compounds in a compound‐pair have approximately equal activities but significantly different structures, are the most prevalent feature of activity landscapes. Hence, from an information theoretic point of view, they are the least informative landscape feature. Nevertheless, similarity cliffs do provide SAR information on potentially new active compound classes, and in that sense they are quite useful in drug discovery programs since they provide alternative possibilities should ADMET or other issues arise during the discovery and earlier preclinical development phases of drug research.
High‐efficiency inverse class‐E power amplifier with envelope tracking technique
Authors: Kam Sang‐Ho , Kwon Oh‐Seung , Lee Mun‐Woo , Jeong Yoon‐Ha 
Published: February 2013

AbstractThis letter describes a highly efficient inverse class‐E power amplifier (PA) with an envelope tracking (ET) technique.To enhance efficiency performances, the PA uses a harmonic control network with double composite right/left‐handed transmission lines. The use of the ET technique also results in high efficiency for the modulated signals. For verifications, the inverse class‐E PA is implemented with a 35‐W GaN HEMT and tested using 3.5 GHz continuous wave (CW) and 802.16e WiMAX signals. With the CW signal, a peak drain efficiency of 79.0% with a power gain of 9.8 dB is achieved at a saturation output power of 45.6 dBm. With WiMAX signals, a drain efficiency of 37.8% with a power‐added efficiency (PAE) of 33.6% is achieved at an average output power of 36 dBm, which is the 9.6 dB back‐off power region, using the ET technique. After the DPD technique, the linearity significantly improved without decreasing efficiency. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 55:866–869, 2013; View this article online at wileyonlinelibrary.com. DOI: 10.1002/mop.27420
Ultra‐wideband band‐pass filter with dual narrow notched bands based on dual‐mode stepped impedance resonator
Authors: Wei Feng , Chen Lei , Wang Zhu Dan , Shi Xiao Wei 
Published: February 2013

AbstractA compact ultra‐wideband (UWB) band‐pass filter (BPF) with dual narrow notch‐bands based on a dual‐mode stepped impedance resonator (SIR) is presented.The proposed UWB filter employed broadside‐coupled microstrip ring‐coplanar waveguide structure. Two notch bands are obtained by introducing one coupled dual‐mode SIR with metalized via hole to ground. Theoretical and simulated results are presented, which are in good agreement with the measured results. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 55:727–730, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.27450
Design of wideband microstrip antenna array at L‐band for synthetic aperture radar applications
Authors: Sharma Devendra Kumar , Pandey Balendu Kumar , Kulshrestha Sanjeev , Chakrabarty S. B. , Jyoti Rajeev 
Published: February 2013

AbstractThis paper presents the design and development of wideband dual‐polarized microstrip array antenna at L‐band for spaceborne synthetic aperture radar (SAR) applications. A new type of electromagnetically coupled microstrip patch fed by strip‐loaded coaxial probe has been used as single antenna element. These radiating elements have been combined to form a 2×6 array antenna using feeder network in stripline configuration. The simulated and measured performance on return loss and radiation parameters has been presented. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 55:903–908, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.27453
Sprayed antenna on cans for WLAN‐RFID tags
Authors: Zuazola I. J. García , Sharma A. , Batchelor J. C. , Angulo I. , Perallos A. , Elmirghani J. M. H. 
Published: February 2013

AbstractA directly printed antenna onto a can body using copper conductive paint and suited for Radio Frequency Identification Wireless LAN (RFID‐WLAN) tag applications is presented. The antenna unit is low‐cost and compact, provide reasonable gain, and serve prospect RFID transponders with a range of IC impedances. The antenna is fabricated with dimensions of 0.18λ × 0.20λ × 3.61e‐4λ and is relaxed from manufacturing. A design study has been carried out to assess the tuning of the characteristic impedance of the antenna. Return loss, load impedance, and radiation patterns are shown. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 55:773–775, 2013; View this article online at wileyonlinelibrary.com. DOI: 10.1002/mop.27458
Fe(III) distribution varies substantially within and between atherosclerotic plaques
Authors: Gustafsson H. , Hallbeck M. , Norell M. , Lindgren M. , Engström M. , Rosén A. , Zachrisson H. 
Published: February 2013

PurposeVulnerable atherosclerotic plaques are structurally weak and prone to rupture, presumably due to local oxidative stress. Redox active iron is linked to oxidative stress and the aim of this study was to investigate the distribution of Fe(III) in carotid plaques and its relation to vulnerability for rupture.MethodsAtherosclerotic plaques from 10 patients (three asymptomatic and seven symptomatic) were investigated. Plaque vulnerability was classified using ultrasound and immunohistochemistry and correlated to Fe(III) measured by electron paramagnetic resonance spectroscopy.ResultsLarge intra‐plaque Fe(III) variations were found. Plaques from symptomatic patients had a higher Fe(III) concentration as compared with asymptomatic plaques (0.36 ± 0.21 vs. 0.06 ± 0.04 nmol Fe(III)/mg tissue, P < 0.05, in sections adjoining narrowest part of the plaques). All but one plaque from symptomatic patients showed signs of cap rupture. No plaque from asymptomatic patients showed signs of cap rupture. There was a significant increase in cap macrophages in plaques from symptomatic patients compared with asymptomatic patients (31 ± 11% vs. 2.3 ± 2.3%, P < 0.01).ConclusionFe(III) distribution varies substantially within atherosclerotic plaques. Plaques from symptomatic patients had significantly higher concentrations of Fe(III), signs of cap rupture and increased cap macrophage activity. Magn Reson Med 71:885–892, 2014. © 2013 Wiley Periodicals, Inc.
A noninvasive tumor oxygenation imaging strategy using magnetic resonance imaging of endogenous blood and tissue water
Authors: Zhang Zhongwei , Hallac Rami R. , Peschke Peter , Mason Ralph P. 
Published: February 2013

PurposeTo present a novel imaging strategy for noninvasive measurement of tumor oxygenation using MR imaging of endogenous blood and tissue water.Theory and MethodsThe proposed approach for oxygen partial pressure (pO2) estimation is based on intravoxel incoherent motion diffusion MRI and the dependence of the blood R2 relaxation rate on the inter‐echo spacing measured using a multiple spin‐echo Carr‐Purcell‐Meiboom‐Gill sequence and weak‐field diffusion model. The accuracy of the approach was validated by comparison with 19F MRI oximetry.ResultsThe results in eight rats at 4.7 T showed that tumors have longer T1 (1980 ± 186 ms) and T2 (59 ± 9 ms) relaxation times, heterogeneous blood volume fraction (0.23 ± 0.1), oxygen saturation level (Y) (0.53 ± 0.12), and pO2 (36 ± 15 mmHg) distributions compared with normal muscle (T1 1480 ± 86 ms, T2 29 ± 2 ms, blood volume fraction 0.22 ± 0.03, Y 0.49 ± 0.06, and pO2 39 ± 5 mmHg). pO2 estimates based on the novel 1H approach were essentially identical with 19F observations.ConclusionThe study indicates that noninvasive measurement of tumor pO2 using 1H MRI derived multiparametric maps is feasible and could become a valuable tool to evaluate tumor hypoxia. Magn Reson Med 71:561–569, 2014. © 2013 Wiley Periodicals, Inc.
Fluorinated Boron‐Dipyrromethene (BODIPY) Dyes: Bright and Versatile Probes for Surface Analysis
Authors: Hecht Mandy , Fischer Tobias , Dietrich Paul , Kraus Werner , Descalzo Ana B. , Unger Wolfgang E. S. , Rurack Knut 
Published: February 2013

AbstractInvited for this month′s cover is the group of Dr. Knut Rurack. The cover picture shows a bright fluorescent dye (green halo) containing a high number of fluorine atoms (blue) for the assessment of reactive amino groups on a glass surface by two complementary surface analytical techniques. For more details, see the Full Paper on p. 25 ff.
Signal intensity changes of the fetal liver on MRI in‐phase and out‐of‐phase sequence
Authors: Li Xiaobing , Brugger Peter C. , Huang Min , Kasprian Gregor , Li Hong , Bettelheim Dieter , Prayer Daniela 
Published: February 2013

ABSTRACTObjectiveTo study signal intensity (SI) of the fetal liver by MRI in‐phase and out‐of‐phase over gestational age (GA).MethodsA total of 91 pregnant women from 19 to 38 gestational weeks were imaged using MRI. Liver‐to‐spleen SI ratios of the right and left fetal liver lobes on in‐phase and out‐of‐phase were measured, calculated, and compared with each other. Curves of liver‐to‐spleen SI ratio of the right and left liver lobe were plotted by GA.ResultsLiver‐to‐spleen SI ratio of the right lobe on in‐phase was different from that of the left liver lobe (t = 3.95; p < 0.001). A statistically significant difference was also found for out‐of‐phase SI ratios (t = 3.69; p < 0.001). Curves of liver‐to‐spleen SI ratio of the fetal liver on in‐phase and out‐of‐phase showed changes against GA.ConclusionLiver‐to‐spleen SI ratio is different between the right and left liver lobe, which probably results from the different blood supply. Curves of liver‐to‐spleen SI ratios between 19 to 38 gestational weeks reflect the changes of decreasing function of blood production by fetal liver. In‐phase and out‐of‐phase may have clinical use in the early detection of disordered fetal growth and metabolism. © 2013 John Wiley & Sons, Ltd.
Application of systems engineering to integrate ageing management into maintenance management of oil and gas facilities
Authors: Pérez Ramírez Pedro A. , Bouwer Utne Ingrid , Haskins Cecilia 
Published: February 2013

AbstractOil companies and relevant institutions are becoming increasingly aware that effective measures must be put in place to extend the life of installations that are approaching, or have passed, the end of their design lifetime. The implementation of ageing management (AM) facilitates ensuring that the degradation of the installations is properly controlled and mitigated, and their integrity is maintained during the life extension. Integrating AM within an existing process such as maintenance management, which shares various goals and objectives with AM, seems to have several advantages over the alternative of creating a new process for AM. A key issue is to identify and optimize the changes in the maintenance management process for addressing and managing ageing. This paper reports on the use of a systems engineering approach and information models for identifying the “as is” and “to be” processes of existing maintenance management of an oil and gas facility in order to fulfill the new requirements for AM. The primary contribution of this report is to demonstrate the power of these models, combined with a systems engineering process, to reverse engineer legacy practices as the basis for introducing new processes, procedures and training. ©2013 Wiley Periodicals, Inc. Syst Eng 16:
Predictors of neonatal morbidity in fetuses with severe isolated congenital diaphragmatic hernia undergoing fetoscopic tracheal occlusion
Authors: Doné E. , Gratacos E. , Nicolaides K. H. , Allegaert K. , Valencia C. , Castañon M. , Martinez J.‐M. , Jani J. , Van Mieghem T. , Greenough A. , Gomez O. , Lewi P. , Deprest J. 
Published: February 2013

ABSTRACTObjectivesTo investigate neonatal morbidity in fetuses with severe congenital diaphragmatic hernia (CDH) treated with fetoscopic endoluminal tracheal occlusion (FETO) and compare it with historical controls with less severe forms of CDH that were managed expectantly.MethodsThis was a prospective, multicenter study on neonatal outcomes and prenatal predictors in 90 FETO survivors (78 left‐sided, 12 right) and 41 controls from the antenatal CDH registry with either severe or moderate hypoplasia who were managed expectantly. We also investigated early neonatal morbidity indicators, including the need for patch repair, duration of mechanical ventilation and supplemental oxygen, age at full enteral feeding and incidence of pulmonary hypertension.ResultsGestational age at delivery was predictive of duration of assisted ventilation (P = 0.046), days on supplemental oxygen (P = 0.019) and age at full enteral feeding (P = 0.020). When delivery took place after 34 weeks' gestation, neonatal morbidity of FETO cases was comparable with that of expectantly managed cases with moderate hypoplasia.ConclusionsFetal intervention for severe CDH is associated with neonatal morbidity that is comparable with that of an expectantly managed group with less severe disease. Copyright © 2013 ISUOG. Published by John Wiley & Sons Ltd.
High-cell-density culture strategies for polyhydroxyalkanoate production: a review
Authors: Ienczak Jaciane Lutz , Schmidell Willibaldo , de Aragão Gláucia Maria Falcão 
Published: February 2013

AbstractThis article gives an overview of high-cell-density cultures for polyhydroxyalkanoate (PHA) production and their modes of operation for increasing productivity. High cell densities are very important in PHA production mainly because this polymer is an intracellular product accumulated in various microorganisms, so a high cellular content is needed for the polymer production. This review describes relevant results from fed-batch, repeated batch, and continuous modes of operation without and with cell recycle for the production of these polymers by microorganisms. Finally, recombinant microorganisms for PHA production, as well future directions for PHA production, are discussed.
“Bounded” Empowerment: Analyzing Tensions in the Practice of Youth‐Led Participatory Research in Urban Public Schools
Authors: Ozer Emily J. , Newlan Sami , Douglas Laura , Hubbard Elizabeth 
Published: February 2013

AbstractThis multi‐method study examines tensions in the practice of youth‐led participatory research (YPAR) in urban high schools among 15 semester‐cohorts. Student participants in the present study were 77 ethnically diverse youth from four high schools in a major metropolitan school district. Data were gathered using systematic classroom observations, interviews with teachers and students involved in the projects, and participant observation. The two most commonly‐constrained phases of the YPAR project were issue selection and action steps. A central tension in the issue selection phase for projects enacted across multiple semester cohorts was the tension between original inquiry and “traction:” Sticking with the same topic enabled sustained building of strategic alliances and expertise for making change, but limited the incoming cohort's power to define the problem to be addressed. In further analyses, we identified processes that promoted student power despite continuity‐related constraints—teachers’ framing and buy‐in strategies, “micro‐power” compensation, and alignment of students’ interests with the prior cohort—as well as constraints in other phases of the projects. This study's findings regarding the promotion of youth power in the face of constraints advance the integration of theory and practice in youth‐led research and have implications for participatory research more broadly.