bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Vitamin D Analog Calcipotriol Combined with Aminolevulinate‐Mediated Photodynamic Therapy for Human Psoriasis: A Proof‐of‐Principle Study
Authors: Maytin Edward V. , Honari Golara , Khachemoune Amor , Taylor Charles R. , Ortel Bernhard , Pogue Brian W. , Sznycer‐Taub Nathaniel , Hasan Tayyaba 
Published: August 2012

AbstractWe previously showed that select agents (methotrexate or vitamin D), when administered as a preconditioning regimen, are capable of promoting cellular differentiation of epithelial cancer cells while simultaneously enhancing the efficacy of 5‐aminolevulinic acid (ALA)‐mediated photodynamic therapy (PDT). In solid tumors, pretreatment with vitamin D simultaneously promotes cellular differentiation and leads to selective accumulation of target porphyrins (mainly protoporphyrin IX, PpIX) within diseased tissue. However, questions of whether or not the effects upon cellular differentiation are inexorably linked to PpIX accumulation, and whether these effects might occur in hyperproliferative noncancerous tissues, have remained unanswered. In this paper, we reasoned that psoriasis, a human skin disease in which abnormal cellular proliferation and differentiation play a major role, could serve as a useful model to test the effects of prodifferentiating agents upon PpIX levels in a nonneoplastic setting. In particular, vitamin D, a treatment for psoriasis that restores (i.e., increases) differentiation, might increase PpIX levels in psoriatic lesions and facilitate their responsiveness to ALA‐PDT. This concept was tested in a pilot study of seven patients with bilaterally matched psoriatic plaques. A regimen in which calcipotriol 0.005 % ointment was applied for six days prior to ALA‐PDT using blue light led to preferential increases in PpIX (ca. 50 %), and reductions in thickness, redness, scaling, and itching in the pretreated plaques. The results suggest that a larger clinical trial is warranted to confirm a role for combination treatments with vitamin D and ALA‐PDT for psoriasis.
Assessment of myelination in hypomyelinating disorders by quantitative MRI
Authors: Dreha‐Kulaczewski Steffi F. , Brockmann Knut , Henneke Marco , Dechent Peter , Wilken Bernd , Gärtner Jutta , Helms G. 
Published: August 2012

AbstractPurpose:To apply myelin‐sensitive quantitative magnetic resonance imaging (MRI) techniques in defined hypomyelinating conditions and to identify spatial patterns of myelination as criteria for characterization of undefined disorders.Materials and Methods:Seven patients were included, based on the diagnosis of mitochondrial cytopathy, Pelizaeus‐Merzbacher disease, GJA12/GJC2‐related Pelizaeus‐Merzbacher‐like disease, hypomyelination with atrophy of the basal ganglia and cerebellum, and leukoencephalopathy with ataxia, delayed dentition, and hypomyelination. The control group comprised 23 children and adolescents (age range 2.6–22.4 years). The 3T MRI protocol consisted of high‐resolution T1‐ and T2‐weighted 3D MRI, diffusion tensor (DTI), and magnetization transfer (MT) imaging. Parameter maps of mean diffusivity, fractional anisotropy, and MT saturation were displayed as pseudocolor overlays and assessed by region‐of‐interest and histogram analysis.Results:Structural MRI revealed widespread signal alterations in white matter, but were hampered by signal heterogeneity. Quantitative DTI and MT reflected the degree of hypomyelination and discriminative patterns of myelination emerged on MT saturation maps.Conclusion:The quantitative parameters in the defined hypomyelination conditions provide additional criteria to further classify undefined white matter disorders. J. Magn. Reson. Imaging 2012; 36:1329–1338. © 2012 Wiley Periodicals, Inc.
T2′‐ and PASL‐based perfusion mapping at 3 Tesla: influence of oxygen‐ventilation on cerebral autoregulation
Authors: Wagner Marlies , Magerkurth Jörg , Volz Steffen , Jurcoane Alina , Singer Oliver C. , Neumann‐Haefelin Tobias , Zanella Friedhelm E. , Deichmann Ralf , Hattingen Elke 
Published: August 2012

AbstractPurpose:To use T2′‐mapping together with Pulsed Arterial Spin Labeling (PASL) providing quantitative information of deoxygenation level and cerebral blood flow (CBF) in the cerebral gray matter to obtain simultaneous information about the cerebral oxygen metabolism and the resulting cerebral vasoreactivity under normoxic and hyperoxic conditions.Materials and Methods:Twelve young, healthy volunteers underwent MRI under normoxic and hyperoxic conditions performing PASL and high‐resolution, motion‐corrected T2* and T2‐mapping to calculate T2′values. Regions of interest (ROI) were placed in the frontoparietal cortex and thalamus by manual and automatic segmentation. For each ROI, mean normoxic T2′‐ and CBF values were extracted and compared with the same parameters assessed under hyperoxic ventilation.Results:A hyperoxic‐induced decrease of the CBF could be shown in the frontoparietal cortex (P = 0.009). The T2 values of frontoparietal cortex decreased under hyperoxic inhalation compared with normoxia (P = 0.01), whereas T2′ remained unchanged.Conclusion:Motion‐corrected high‐resolution T2′‐maps can be used together with PASL to evaluate the DeoxyHb content in relation to CBF in the cerebral gray matter. We could show that cortical CBF decreases under hyperoxic inhalation in healthy young subjects, whereas the T2′ values remained constant. These data suggest that hyperoxic‐induced vasoconstriction may protect the brain against hyperoxemia. J. Magn. Reson. Imaging 2012; 36:1347–1352. © 2012 Wiley Periodicals, Inc.
Prognosis and predictors of ACL reconstructions using the MOON cohort: A model for comparative effectiveness studies
Authors: Spindler Kurt P. , Parker Richard D. , Andrish Jack T. , Kaeding Christopher C. , Wright Rick W. , Marx Robert G. , McCarty Eric C. , Amendola Annunziato , Dunn Warren R. , Huston Laura J. , Harrell Frank E. ,   MOON Group
Published: August 2012

AbstractInjury to the anterior cruciate ligament (ACL) threatens an active lifestyle and exposes the patient to risk of early osteoarthritis (OA). ACL reconstruction is typically chosen by individuals to allow a return to their previous work and sports activities. Primary ACL reconstruction (ACLR) has in general been effective at restoring functional stability, but patients' modifiable predictors of both short‐ and long‐term validated outcomes and OA are largely unknown. The Multicenter Orthopaedic Outcomes Network (MOON) consortium was established in 2002 to enroll and longitudinally follow a population cohort of ACL reconstructed patients. The objective was to establish patient‐specific predictive models of clinically important outcomes. Over the past 10 years, the overarching aims of this NIAMS‐funded prospective multicenter cohort of ACL reconstructions has been threefold: (1) to identify both short‐ and long‐term prognosis and predictors of sports function, activity level, and general health through validated patient‐reported outcomes, (2) to identify the symptoms and signs of OA, and (3) to quantify the incidence of ACL reconstruction graft and/or contralateral ACL failures and additional surgical procedures. This manuscript summarizes the Kappa Delta Ann Doner Vaughan Award paper and presentation at the 2012 ORS/AAOS Annual Meeting. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31:2–9, 2012
Refixation of the supraspinatus tendon in a rat model—influence of continuous growth factor application on tendon structure
Authors: Buchmann Stefan , Sandmann Gunther H. , Walz Lars , Hoppe Henriette , Beitzel Knut , Wexel Gabriele , Tian Weiwei , Winter Gerhard , Imhoff Andreas B. 
Published: August 2012

AbstractThe purpose was to evaluate histological changes of the supraspinatus tendon (SSP) after refixation under continuous growth factor application over 20 days in comparison to the native healing process. In a chronic rat tendon tear model (15 rats/group), a transosseous SSP refixation was performed and growth factors (control, G‐CSF, b‐FGF, combination) were continuously released into the subacromial space by an osmotic pump. Tendon healing was evaluated histologically by a modified MOVIN‐Score, and Collagen I/III content was determined by immunohistology at 6 weeks. A modified MOVIN sum score showed significant lower counts for G‐CSF and b‐FGF in comparison to the control group (p = 0.050/p = 0.027) and the combined group (p = 0.050/p = 0.043). Collagen III was significantly reduced in the combined group compared to the control group (p = 0.028). Collagen I showed no significant differences. The Collagen I/III ratio was nearly doubled for b‐FGF and the combined group compared to the control. At the study endpoint, 33% of pump dislocations were detected. The continuous application of both isolated growth factors (G‐CSF/b‐FGF) achieved improved tendon‐remodeling. However, the continuous application via an osmotic pump showed a relative high dislocation rate when applied in the rat model. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 300–305, 2013
Process length variation of the cyst of the dinoflagellate Protoceratium reticulatum in the North Pacific and Baltic‐Skagerrak region: calibration as an annual density proxy and first evidence of pseudo‐cryptic speciation
Authors: Mertens Kenneth Neil , Bringué Manuel , Van Nieuwenhove Nicolas , Takano Yoshihito , Pospelova Vera , Rochon Andre , De Vernal Anne , Radi Taoufik , Dale Barrie , Patterson R. Timothy , Weckström Kaarina , Andrén Elinor , Louwye Stephen , Matsuoka Kazumi 
Published: August 2012

AbstractProcess length variation of cysts of the dinoflagellate Protoceratium reticulatum (Claparède et Lachmann) Bütschli in surface sediments from the North Pacific was investigated. The average process length showed a significant inverse relation to annual seawater density: σt annual = −0.8674 × average process length + 1029.3 (R2 = 0.84), with a standard error of 0.78 kg m−3. A sediment trap study from Effingham Inlet in British Columbia revealed the same relationship between average process length and local seawater density variations. In the Baltic–Skagerrak region, the average process length variation was related significantly to annual seawater density: σt annual = 3.5457 × average process length − 993.28 (R2 = 0.86), with a standard error of 3.09 kg m−3. These calibrations cannot be reconciled, which accentuates the regional character of the calibrations. This can be related to variations in molecular data (small subunit, long subunit and internal transcribed spacer sequences), which show the presence of several genotypes and the occurrence of pseudo‐cryptic speciation within this species. Copyright © 2012 John Wiley & Sons, Ltd.
Climatic variations in MIS 11 recorded by stable isotopes and trace elements in a French tufa (La Celle, Seine Valley)
Authors: Dabkowski Julie , Limondin‐Lozouet Nicole , Antoine Pierre , Andrews Julian , Marca‐Bell Alina , Robert Vincent 
Published: August 2012

AbstractMarine Isotope Stage (MIS) 11 palaeoclimate has so far been documented in marine and ice sheet isotopic records. However, excepting some lacustrine pollen records, very little is known about palaeoclimatic conditions in continental areas. This study uses geochemical records in calcareous tufa deposits from rivers as a basis for reconstructing temperate palaeoclimatic conditions. Tufa deposits are now proven to record high‐quality palaeoclimatic information in recent to Holocene deposits. Work on older interglacial tufas is just starting and in this paper we present the first comprehensive results from a MIS 11 tufa. The tufa comes from the Seine Valley (La Celle, northern France). Geochemical data in the tufa calcite are interpreted to record primarily air temperature (δ18O) and humidity (δ13C and Mg/Ca and Sr/Ca). The combined data identify a warm and wet climatic optimum followed by two temperature decreases associated with oscillations in humidity. These marked climatic variations recorded through the La Celle profile are strongly coherent with the palaeoenvironmental reconstructions from malacological data. The abrupt climatic and environmental events recorded could be related to short‐term degradation of vegetation cover in Europe, which is itself controlled by global palaeoclimatic events. Copyright © 2012 John Wiley & Sons, Ltd.
Sensory quality and appropriateness of raw and boiled Jerusalem artichoke tubers (Helianthus tuberosus L.)
Authors: Bach Vibe , Kidmose Ulla , Thybo Anette K , Edelenbos Merete 
Published: August 2012

AbstractBACKGROUND: The aim of the present study was to investigate the sensory attributes, dry matter and sugar content of five varieties of Jerusalem artichoke tubers and their relation to the appropriateness of the tubers for raw and boiled preparation.RESULTS: Sensory evaluation of raw and boiled Jerusalem artichoke tubers was performed by a trained sensory panel and a semi‐trained consumer panel of 49 participants, who also evaluated the appropriateness of the tubers for raw and boiled preparation. The appropriateness of raw Jerusalem artichoke tubers was related to Jerusalem artichoke flavour, green nut flavour, sweetness and colour intensity, whereas the appropriateness of boiled tubers was related to celeriac aroma, sweet aroma, sweetness and colour intensity. In both preparations the variety Dwarf stood out from the others by being the least appropriate tuber.CONCLUSION: A few sensory attributes can be used as predictors of the appropriateness of Jerusalem artichoke tubers for raw and boiled consumption. Knowledge on the quality of raw and boiled Jerusalem artichoke tubers can be used to inform consumers on the right choice of raw material and thereby increase the consumption of the vegetable. © 2012 Society of Chemical Industry
High Internal Phase Emulsion Templating – A Path To Hierarchically Porous Functional Polymers
Authors: Pulko Irena , Krajnc Peter 
Published: August 2012

AbstractRecently, a series of new monomers and polymerization mechanisms has been applied to the templating of high internal phase emulsions (HIPEs) providing a route to hierarchically porous materials with a range of functionalities and applications. The high degree of control over the pore size is another attractive feature of these materials. Usually, the continuous phase contains monomers, the droplet phase is used to template the large, primary pores, which are interconnected by secondary pores. The addition of nonpolymerizable components to the continuous phase can result in phase separation during polymerization and tertiary pores. Applications include polymer supports for catalysis and synthesis, separation and filtration, cell culture media, enzyme supports, and structural and isolation applications.
Inflammation‐mediated abrogation of androgen signaling: An in vitro model of prostate cell inflammation
Authors: Debelec‐Butuner Bilge , Alapinar Cansu , Varisli Lokman , Erbaykent‐Tepedelen Burcu , Hamid Syed Muhammad , Gonen‐Korkmaz Ceren , Korkmaz Kemal Sami 
Published: August 2012

AbstractAs a link between inflammation and cancer has been reported in many studies, we established an in vitro model of prostatic inflammation to investigate the loss of androgen receptor (AR)‐mediated signaling in androgen responsive prostate cell lines. First, the U937 monocyte cell line was differentiated into macrophages using phorbol acetate (PMA), and cells were induced with lipopolysaccharide (LPS) for cytokine secretion. Next, the cytokine levels (TNFα, IL‐6, and IL1β) in conditioned media (CM) were analyzed. Prostate cells were then fed with CM containing varying concentrations of TNFα, and IkB degradation, nuclear factor kappa B (NFκB) translocation and transactivation, and the expression of matrix metalloproteinase‐8 (MMP8) and matrix metalloproteinase‐9 (MMP9) were then assessed. As a result of CM treatment, ubiquitin‐mediated AR degradation, which was restored using anti‐TNFα antibody neutralization, led to both a decrease in KLK4, PSA, and NKX3.1 expression levels and the upregulation of GPX2. In addition to the loss of AR, acute and chronic CM exposure resulted in p53 degradation and consequent p21 downregulation, which was also restored by either androgen administration or ectopic NKX3.1 expression via the stabilization of MDM2 levels in LNCaP cells. Additionally, CM treatment enhanced H2AX(S139) phosphorylation (a hallmark of DNA damage) and genetic heterogeneity in the absence of androgens in prostate cells without activating mitochondrial apoptosis. Thus, the data suggest that inflammatory cytokine exposure results in the loss of AR and p53 signaling in prostate cells and facilitates genetic heterogeneity via ROS accumulation to promote prostate carcinogenesis. © 2012 Wiley Periodicals, Inc.
Serine 727 phosphorylation of STAT3: An early change in mouse hepatocarcinogenesis induced by neonatal treatment with diethylnitrosamine
Authors: Miyakoshi Masaaki , Yamamoto Masahiro , Tanaka Hiroki , Ogawa Katsuhiro 
Published: August 2012

AbstractSTAT3 activation is involved in development and progression of hepatocellular carcinoma (HCC). We investigated STAT3 activation during hepatocarcinogenesis induced by neonatal diethylnitrosamine (DEN) treatment in mice. Nuclear accumulation and phosphorylation of STAT3 were detected in altered hepatocyte foci in the early stages as well as adenomas and HCCs in the late stages. Although total STAT3 levels were the same between the hepatic lesions and normal livers, S727‐phosphorylated STAT3 was enhanced in adenomas and HCCs, whereas Y705‐phosphorylated STAT3 was detected mainly in HCCs. In mouse HCC cell lines, although both S727 and Y705 remained un‐ or hypophosphorylated under serum‐free conditions, fetal bovine serum (FBS) induced strong S727/weak Y705 phosphorylation, STAT3 nuclear accumulation and cell proliferation, whereas IL‐6 treatment without FBS caused Y705 phosphorylation without S727 phosphorylation, STAT3 nuclear accumulation or cell proliferation. When HCCs were simultaneously treated with FBS/IL‐6, selective suppression of S727 phosphorylation by an MEK inhibitor prevented STAT3 nuclear accumulation and cell proliferation. Furthermore, an S727 phosphorylation‐deficient STAT3 mutant (S727A) had a diminished capacity to accumulate in the nucleus when compared with wild‐type (WT) or the phosphorylation‐mimic mutant (S727D) following treatment with FBS/IL‐6. After treatment with FBS/IL‐6, the cells expressing the S727A mutant proliferated more slowly than those expressing WT or S727D mutant. In contrast, suppression of Y705 phosphorylation by a JAK inhibitor in the FBS/IL‐6 treated cells did not affect STAT3 nuclear accumulation or cell proliferation. Taken together, these data demonstrate that STAT3 activation, mainly through S727 phosphorylation, contributes to the DEN‐induced hepatocarcinogenesis at the earliest stages. © 2013 Wiley Periodicals, Inc.
Nurse reported quality of care: A measure of hospital quality
Authors: McHugh Matthew D. , Stimpfel Amy Witkoski 
Published: August 2012

AbstractAs the primary providers of round‐the‐clock bedside care, nurses are well positioned to report on hospital quality of care. Researchers have not examined how nurses' reports of quality correspond with standard process or outcomes measures of quality. We assess the validity of evaluating hospital quality by aggregating hospital nurses' responses to a single item that asks them to report on quality of care. We found that a 10% increment in the proportion of nurses reporting excellent quality of care was associated with lower odds of mortality and failure to rescue; greater patient satisfaction; and higher composite process of care scores for acute myocardial infarction, pneumonia, and surgical patients. Nurse reported quality of care is a useful indicator of hospital performance. © 2012 Wiley Periodicals, Inc. Res Nurs Health 35:566–575, 2012
A Unique Case of Prone Position in the Primary Cremation Tomb 252 of Monte Sirai Necropolis (Carbonia, Sardinia, Italy)
Authors: Piga G. , Malgosa A. , Thompson T. J. U. , Guirguis M. , Enzo S. 
Published: August 2012

AbstractA series of graves belonging to the Phoenician period were investigated after the campaign excavation of 2007 in the Phoenician‐Punic necropolis of Monte Sirai (Carbonia, Sardinia, Italy). One tomb in particular (labelled 252) is presented here because of its uniqueness. Tomb 252 contained the cremated remains of an individual, probably male, and comparison of the excavation records alongside reconstruction of the bone material itself makes clear that the individual was cremated in a prone position. It represents the first case of prone cremation reported in the literature.To investigate whether the temperature of the cremation was homogenous across the entire body, we have conducted a combined physical–chemical analysis using X‐ray diffraction spectroscopy and Fourier transform infrared. Copyright © 2012 John Wiley & Sons, Ltd.
Intensifying Social Exchange Relationships in Public Organizations: Evidence from a Randomized Field Experiment
Authors: Jakobsen Morten , Andersen Simon Calmar 
Published: August 2012

AbstractIn accordance with social exchange theory, prominent streams of management research emphasize the importance of reciprocal exchange relationships between organizations and their employees. When employees perceive themselves as supported by the organization, they reciprocate with increased work motivation. However, we do not know how this knowledge can be developed into management initiatives that increase public employees’ perceived support, because severe endogeneity problems make it difficult to estimate the effect of organizational support on employee commitment outside the laboratory. We use a randomized field experiment involving more than 800 public employees to estimate the effect. We find no average effect of the organizational support treatment on the employees’ perceived organizational support. Yet, a subgroup analysis shows a positive treatment effect when the employees’ local front‐line managers felt less supported prior to the intervention. We discuss the implications for theory and management practice. ©2012 by the Association for Public Policy Analysis and Management.