bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of genetic epilepsies
Authors: Hedrich U. , Maljevic S. , Lerche H. 
Published: November 2013

Abstract
Epilepsy is one of the most common neuro­logical disorders. As early as the time of Hip­pocrates (460-370 BC), it was reported on as the “holy disease”. Today it is known that an epileptic seizure is a consequence of synchro­nous discharges of neuronal populations in the brain, which abruptly and usually without an observable cause evoke involuntary be­havioural dysfunction or impaired conscious­ness. Epilepsies can have various causes and lead to extensive implications for the every­day life of affected patients. Up to 50% of all epilepsies are caused by genetic defects, in particular the so-called idiopathic epilep­sies, which occur without easily observable structural alterations in the brain. Genetical­ly caused dysfunctions of neuronal ion chan­nels play a central role in the formation of such epilepsies. The ion channels control the ion flux over the cell membrane of neurons and thus present the basis for the excitabili­ty of these neurons. Therefore, medications used in epilepsy treatment affect predomi­nantly ion channels. However, common an­ticonvulsants have limited success, not only because one third of epilepsy patients exhibit pharmacoresistance, but also because of the secondary effects which can dramatically af­fect their quality of life. Furthermore, current therapeutic approaches are mainly symp­tomatic and do not act on the epileptogenic mechanisms, which are still largely unknown. In this review article we will highlight the cur­rent main topics of our research on genetical­ly caused epilepsies, their pathomechanisms and therapeutic options.
Neuronal networks in the spotlight
Authors: Helmchen F. , Hübener M. 
Published: November 2013

Abstract
The brain’s astounding achievements regard­ing movement control and sensory process­ing are based on complex spatiotemporal ac­tivity patterns in the relevant neuronal net­works. Our understanding of neuronal net­work activity is, however, still poor, not least because of the experimental difficulties in di­rectly observing neural circuits at work in the living brain (in vivo). Over the last decade, new opportunities have emerged-especial­ly utilizing two-photon microscopy-to in­vestigate neuronal networks in action. Cen­tral to this progress was the development of fluorescent proteins that change their emis­sion depending on cell activity, enabling the visualization of dynamic activity patterns in local neuronal populations. Currently, genet­ically encoded calcium indicators, proteins that indicate neuronal activity based on ac­tion potential-evoked calcium influx, are be­ing increasingly used. Long-term expression of these indicators allows repeated moni­toring of the same neurons over weeks and months, such that the stability and plastici­ty of their functional properties can be char­acterized. Furthermore, permanent indicator expression facilitates the correlation of cel­lular activity patterns and behavior in awake animals. Using examples from recent studies of information processing in the mouse neo­cortex, we review in this article these fasci­nating new possibilities and discuss the great potential of the fluorescent proteins to eluci­date the mysteries of neural circuits.
Beyond indices: The potential of fuzzy set ideal type analysis for cross-national analysis of policy outcomes
Authors: Hudson John , Kühner Stefan 
Published: November 2013

AbstractLeague tables ranking performance outcomes within or between countries have become commonplace in most policy sectors in recent decades and there are numerous examples to be found in practice. UNICEF's 2007 Overview of Child Well-Being in Rich Countries offered a comprehensive and widely cited comparative analysis of children's well being in 21 of the richest countries of the world. Utilising an additive index the authors distilled a large amount of quantitative data relating to children's well being and were able to provide the most comprehensive snapshot of outcomes to date. Whilst an advantage of the method — and certainly a key factor in generating media coverage — was the way it allowed for a ranking of nations, recent debates in the comparative policy analysis literature have pointed to the advantages of methods that aim to classify nations into qualitatively distinct types rather than ranking them in league tables. These debates have particular force when multiple components of analysis are conceptually distinct or cases have widely varying contexts. Fuzzy set ideal type analysis (FSITA) has become an increasingly popular alternative approach to the additive index, precisely because it addresses these concerns. In this paper we explore the potential for using FSITA for the comparative analysis of children's well-being. Drawing on the same data and conceptual foundations as the 2007 UNICEF study we explore the potential advantages of utilising a diversity oriented method such as FSITA as tool for policy evaluation that eschews ranking in favour of classifying.
Roast Beef, the New Poor Law, and the British Nation, 1834–63
Authors: Durbach Nadja 
Published: November 2013

AbstractIn the early years of the New Poor Law, workhouses were explicitly prohibited from serving roast beef and plum pudding to inmates. Historians have recently begun to focus increased attention on the cultural meanings invested in specific food products and the politics of their production, distribution, and consumption. Unpacking the contentious disputes between local and central Poor Law authorities over the provision of roast beef to workhouse inmates similarly reorients the discussion of the pauper diet to address, not the amount or quality of food provided, but rather the cultural politics of what exactly a pauper was allowed to eat. A study of when and why paupers were and were not furnished with a festive meal of what was often termed “Old English Fare” provides a way of rethinking the place of the poor within local and national communities at a moment when attitudes toward poverty were undergoing profound changes.
Feeding in the Workhouse: The Institutional and Ideological Functions of Food in Britain, ca. 1834–70
Authors: Miller Ian 
Published: November 2013

AbstractHow adequate was the mid-Victorian workhouse diet? Basing their arguments on modern nutritional analyses of dietary tables, some historians have concluded that the workhouse diet fulfilled the basic nutritional needs of inmates and that the idea that workhouse dietary regimes were inadequate is the result of a “mythology” created by contemporaries—including Charles Dickens. In these accounts, Dickens's infamous scene where Oliver Twist becomes so overwhelmed with hunger that he asks for more food is construed as an exaggerated rendering of workhouse life. This article argues that efforts to impose modern nutritional techniques onto past configurations can produce misleading results and generate simplistic historical interpretations. The cultural categories historically surrounding food demand thorough attention and must be reconciled with modern scientific approaches if the boundaries between workhouse realities and mythologies are to be rendered less obscure.
Long-chain n-3 DHA reduces the extent of skeletal muscle fatigue in the rat in vivo hindlimb model
Authors: Peoples Gregory E. , McLennan Peter L. 
Published: November 2013

Dietary fish oil modifies skeletal muscle membrane fatty acid composition and oxygen efficiency similar to changes in the myocardium. Oxygen efficiency is a key determinant of sustained force in mammalian skeletal muscle. Therefore, in the present study, we tested the effects of a fish-oil diet on skeletal muscle fatigue under the stress of contraction using the rat in vivo autologous perfused hindlimb model. For 8 weeks, male Wistar rats were fed a diet rich in saturated fat (SF), a diet rich in n-6 PUFA or a diet rich in long-chain (LC) n-3 PUFA DHA derived from fish oil. In anaesthetised, mechanically ventilated rats, with their hindlimbs perfused with arterial blood at a constant flow, the gastrocnemius–plantaris–soleus muscle bundle was stimulated via sciatic nerve (2 Hz, 6–12 V, 0·05 ms) to contract repetitively for 30 min. Rats fed the n-3 PUFA diet developed higher maximum twitch tension than those fed the SF and n-6 PUFA diets (P< 0·05) and sustained twitch tension through more repetitions before the tension declined to 50 % of the maximum twitch tension (P< 0·05). The n-3 PUFA group used less oxygen for tension developed and produced higher venous lactate concentrations with no difference in glycogen utilisation compared with the SF and n-6 PUFA groups. These results further support that incorporation of DHA into skeletal muscle membranes increases the efficiency of oxygen use over a range of contractile force and this is expressed as a higher sustained force and prolonged time to fatigue.
An audit of ‘dead ear’ after ear surgery
Authors: Prinsley P 
Published: November 2013

AbstractIntroduction:‘Dead ear’ is a rare and serious complication of ear surgery. This paper presents an audit of this complication.Method:Over 6 years, data for all 617 middle-ear operations performed under the care of a single consultant were recorded for the International Otology Audit. All cases of dead ear were identified and assessed.Results:A post-operative dead ear occurred in 6 cases (approximately 1 per cent). No cases of post-operative dead ear occurred following the 83 otosclerosis operations and the 62 children's procedures. Amongst 187 adult patients undergoing mastoid surgery for cholesteatoma, there were 5 cases of post-operative dead ear (2.7 per cent of cases).Conclusion:The occurrence of dead ear after cholesteatoma surgery in adults is less rare than previously thought. This has implications for the surgical consenting process. The current series suggests that, whilst dead ear is often avoidable, it is sometimes inevitable.
Evolution of parasitism along convergent lines: from ecology to genomics
Authors: POULIN ROBERT , RANDHAWA HASEEB S. 
Published: November 2013

SUMMARYFrom hundreds of independent transitions from a free-living existence to a parasitic mode of life, separate parasite lineages have converged over evolutionary time to share traits and exploit their hosts in similar ways. Here, we first summarize the evidence that, at a phenotypic level, eukaryotic parasite lineages have all converged toward only six general parasitic strategies: parasitoid, parasitic castrator, directly transmitted parasite, trophically transmitted parasite, vector-transmitted parasite or micropredator. We argue that these strategies represent adaptive peaks, with the similarities among unrelated taxa within any strategy extending to all basic aspects of host exploitation and transmission among hosts and transcending phylogenetic boundaries. Then, we extend our examination of convergent patterns by looking at the evolution of parasite genomes. Despite the limited taxonomic coverage of sequenced parasite genomes currently available, we find some evidence of parallel evolution among unrelated parasite taxa with respect to genome reduction or compaction, and gene losses or gains. Matching such changes in parasite genomes with the broad phenotypic traits that define the convergence of parasites toward only six strategies of host exploitation is not possible at present. Nevertheless, as more parasite genomes become available, we may be able to detect clear trends in the evolution of parasitic genome architectures representing true convergent adaptive peaks, the genomic equivalents of the phenotypic strategies used by all parasites.
Distribution of gymnosomatous pteropods in western Antarctic Peninsula shelf waters: influences of Southern Ocean water masses
Authors: Suprenand P.M. , Jones D.L. , Torres J.J. 
Published: November 2013

ABSTRACTDistributions of gymnosomatous pteropods,Spongiobranchaea australisandClione antarctica, were determined at six sites along a latitudinal gradient in western Antarctica Peninsula shelf waters using vertically stratified trawls. Hydrographic data were collected at the same sites with conductivity-temperature-depth casts, and correlations of explanatory variables to gymnosome distributions were determined using statistical analyses performed in Matlab, a high level programming software to conduct numerical computation and visualisation. Explanatory variables included sampling site, latitude, longitude and depth, seawater temperature, salinity and density, Southern Ocean Antarctic Surface Water, Winter Water, Upper Circumpolar Deep Water and warm transitional waters, as well as oceanographic remote sensing data for coloured dissolved organic matter, chlorophyllaconcentration, normalised fluorescence line height, nighttime sea surface temperature, photosynthetically active radiation, particulate inorganic carbon, particulate organic carbon, daytime sea surface temperature and daily sea ice concentration. Hydrographic data revealed that warmer water masses were prevalent along the western Antarctic Peninsula, and the distributions of both gymnosome species were primarily influenced by water masses, temperature, sampling site and latitude. As a consequence, distributional shifts of gymnosomes are predicted in response to the current warming trends.
Capacity building with older people through local authority and third-sector partnerships
Authors: DALZIEL ROBERT , WILLIS MARTIN 
Published: November 2013

ABSTRACTIn May 2010 a Conservative and Liberal Democrat Coalition Government was elected in the United Kingdom, which immediately started to plan a programme of wide-ranging cuts in public spending. However, in the face of severe economic problems the new government retained the outgoing government's emphasis on active ageing. This paper examines capacity-building partnerships between local authorities and third-sector organisations in LinkAge Plus (LAP) pilot areas in England, which were set up to find better ways to meet the needs of older people and empower them to become active citizens. The study on which this paper reports used theory on partnerships and collaboration to interrogate LAP pilot evaluation reports, along with current thinking on capacity building and work designed to improve services and outcomes for older people. The main findings are that capacity building in partnerships stimulated joined up working, which resulted in improved knowledge and skills in providing existing services. At the same time, new services emerged that meant older people were more involved in networking activities and social capital was created through their engagement in policy making, identifying needs, service design and finding solutions to problems. However, there were few instances of ideological activity that challenged established values and ways of working to go beyond traditional health and social care approaches in the delivery of services for older people. The potential impact of ongoing cuts in public spending are also considered.
Can fruit pulp meet the calcium needs of tropical frugivorous passerines during reproduction?
Authors: Foster Mercedes S. 
Published: November 2013

Abstract:Calcium is an important nutrient for birds, especially for eggshell production and the mineralization of the skeleton of developing young. In temperate regions insects and seeds that form the bulk of the diet of breeding passerines do not contain sufficient calcium to meet the needs of breeding females and young; these birds obtain their calcium by eating snails, woodlice and millipedes. Little is known about the way tropical frugivorous passerines meet their calcium needs. The calcium contents of fruits of 34 species eaten by birds during the breeding season in mature floodplain forest in south-eastern Peru were determined. The suitability of these fruits as calcium sources for hypothetical 10-g and 25-g birds and for 26 species of frugivore that breed at the study site was evaluated based on bird body weight and on the rictal width of the bill, which influences feeding. Fruits of five species in the study area appear to be suitable sources of calcium for birds ≤25 g, although those ofFicus killipii(Moraceae) are likely optimal. The latter contain a lot of calcium (2.48% dry weight of pulp orc. 4.15 mg per pulp per fruit) and are small enough (mean diam. = 9 mm) for most birds to swallow whole, and trees produce large crops. Snails, woodlice and millipedes are common in the area and also contain sufficient calcium to meet birds’ needs. In addition, birds could likely meet their calcium needs through geophagy at natural mineral licks.
The omnivorous collared peccary negates an insectivore-generated trophic cascade in Costa Rican wet tropical forest understorey
Authors: Michel Nicole L. , Sherry Thomas W. , Carson Walter P. 
Published: November 2013

Abstract:Insectivorous birds and bats often protect plants through density- and trait-mediated cascades, but the degree to which insectivores reduce herbivorous arthropods and leaf damage varies among systems. Top-down interaction strength may be influenced by the biotic and abiotic context, including the presence of vegetation-disturbing animals. We tested two hypotheses: (1) insectivorous birds and bats initiate trophic cascades in tropical rain-forest understorey; and (2) the native, omnivorous collared peccary (Pecari tajacu) negates these cascades via non-trophic effects. We studied the top-down effects of birds and bats on understorey plants in north-eastern Costa Rica using 60 netted exclosures within and outside existing peccary exclosures. Excluding birds and bats increased total arthropod densities by half, both with and without peccaries. Bird/bat exclosures increased Diptera density by 28% and leaf damage by 24% without peccaries, consistent with a trophic cascade. However, bird/bat exclosures decreased Diptera density by 32% and leaf damage by 34% with peccaries, a negation of the trophic cascade. Excluding peccaries increased leaf damage by 43% on plants without birds and bats. This is the first study, to our knowledge, to demonstrate that the non-trophic activity of an omnivorous ungulate can reverse a trophic cascade.
Hospitals' Responses to Administrative Cost-containment Policy in Urban China: The Case of Fujian Province
Authors: Jingwei He Alex , Qian Jiwei 
Published: November 2013

AbstractThe patterns of hospitals' behavioural changes in response to different insurance systems and payment arrangements have been well documented in the literature on health economics and policy. To understand these changes, it is necessary to look at the shifts in fundamental economic incentives. Meanwhile, hospital practices are also subject to adjustment when administrative tools are realigned. This article examines the dynamics of a health policy campaign started in 2005 by a Chinese provincial health administration that was committed to containing health expenditures using administrative measures. Through a combination of qualitative in-depth interviews and quantitative panel data analysis comprising 30 public hospitals in the sample, this article finds that by revising the structure of administrative measures on the supply side, the Chinese health bureaucracy is able to curb rapid cost inflation in the short term. However, while having to meet the cost control mandate imposed by the health administration, Chinese public hospitals still managed to defend their economic interests by engaging in various unintended opportunistic behaviour. This article analyses a panel database from Fujian province and reveals the strategies adopted by public hospitals and considers their implications for China's ongoing national healthcare reform.
Influenza in hospitalized children in Ireland in the pandemic period and the 2010/2011 season: risk factors for paediatric intensive-care-unit admission
Authors: REBOLLEDO J. , IGOE D. , O'DONNELL J. , DOMEGAN L. , BOLAND M. , FREYNE B. , McNAMARA A. , MOLLOY E. , CALLAGHAN M. , RYAN A. , O'FLANAGAN D. 
Published: November 2013

SUMMARYInfluenza causes significant morbidity and mortality in children. This study's objectives were to describe influenza A(H1N1)pdm09 during the pandemic, to compare it with circulating influenza in 2010/2011, and to identify risk factors for severe influenza defined as requiring admission to a paediatric intensive care unit (PICU). Children hospitalized with influenza during the pandemic were older, and more likely to have received antiviral therapy than children hospitalized during the 2010/2011 season. In 2010/2011, only one child admitted to a PICU with underlying medical conditions had been vaccinated. The risk of severe illness in the pandemic was higher in females and those with underlying conditions. In 2010/2011, infection with influenza A(H1N1)pdm09 compared to other influenza viruses was a significant risk factor for severe disease. An incremental relationship was found between the number of underlying conditions and PICU admission. These findings highlight the importance of improving low vaccination uptake and increasing the use of antivirals in vulnerable children.
Comparative expression analysis of embryonic development-related genes at different stages of parthenogenetic andin vitrofertilized embryos in caprine
Authors: Singh Renu , Kumar Kuldeep , Ranjan R. , Kumar Manish , Yasotha T. , Singh R.K. , Das B.C. , Sarkar M. , Bag Sadhan 
Published: November 2013

SummaryAberrant gene expression occurs in parthenogenetic embryos due to abnormal epigenetic modifications in the genome that probably diminish viability and enhance developmental abnormalities in these embryos. In the present study, five developmentally important genes (HPRT1,Cx43,Sox2,MestandIGF2R) were analysed at different stages in parthenotes (haploid and diploid) and compared with similar stages inin vitrofertilized (IVF) embryos. The results indicated that in haploid parthenotes expression ofHPRT1was upregulated (P< 0.05) only at the 2–4-cell stage whereasCx43expression was significantly (P< 0.05) downregulated in all stages as compared with the control. However, expression of this gene was upregulated (P< 0.05) in 2–4-cell and morula stages of diploid parthenotes. Expression ofSox2was significantly (P< 0.05) downregulated in morula stage haploid parthenotes, whereas it was upregulated (P< 0.05) in 8–16-cell stage diploid embryos. The expression ofMestwas upregulated (P< 0.05) at the 2–4-cell stage of both haploid and diploid parthenotes, whereas it was downregulated in 8–16-cell stage diploid embryos as compared with control.IGF2Rexpression was upregulated (P< 0.05) only in morula stage haploid and diploid parthenote as compared with control. These results indicate that parthenogenetic embryos showed aberrant gene expression of developmentally important genes such asHPRT1,Cx43,Sox2,MestandIGF2Rin comparison with IVF embryos, this finding may be one of the major reasons for the poor developmental competence of parthenogenetic embryos.
Leaf Anatomy and Histochemistry of Three Species ofFicussect.AmericanaeSupported by Light and Electron Microscopy
Authors: Araújo Nathalia Diniz , Coelho Victor Peçanha M. , Ventrella Marília Contin , Agra Maria de Fátima 
Published: November 2013

AbstractIn this work the leaf anatomy of three species ofFicussectionAmericanae(Miq.) Miq. from Brazil, whose leaves and latex are used in folk medicine is reported. The work was carried out using light and scanning electron microscopy in order to characterize these species and to evaluate their taxonomic significance, and also contribute to the quality control of their ethnodrugs. The three species (Ficus cyclophylla,Ficus elliotiana, andFicus caatingae) showed hypostomatic leaves, anomocytic stomata, straight epidermal cell outlines, and a dorsiventral mesophyll. Some micro-morphological characters such as density and distribution of epicuticular waxes, glandular trichomes, the length and width of stomata, as well as the palisade of mesophyll and petiole outlines proved to be the most useful and distinctive characters for the separation of species. These may contribute as additional support for the taxonomy of the section and for the quality control of their ethnodrugs.