bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electromechanical Assessment of Human Knee Articular Cartilage with Compression-Induced Streaming Potentials
Authors: Becher Christoph , Ricklefs Marcel , Willbold Elmar , Hurschler Christof , Abedian Reza 
Published: August 2015

Purpose: To assess the electromechanical properties of human knee articular cartilage with compression-induced streaming potentials for reliability among users and correlation with macroscopic and histological evaluation tools and sulfated glycosaminoglycan (sGAG) content. Methods: Streaming potentials are induced in cartilage in response to loading when mobile positive ions in the interstitial fluid temporarily move away from negatively charged proteoglycans. Streaming potential integrals (SPIs) were measured with an indentation probe on femoral condyles of 10 human knee specimens according to a standardized location scheme. Interobserver reliability was measured using an interclass correlation coefficient (ICC). The learning curves of 3 observers were evaluated by regression analysis. At each SPI measurement location the degradation level of the tissue was determined by means of the International Cartilage Repair Society (ICRS) score, Mankin score, and sGAG content. Results: The computed ICC was 0.77 (0.70-0.83) indicating good to excellent linear agreement of SPI values among the 3 users. A significant positive linear correlation of the learning index values was observed for 2 of the 3 users. Statistically significant negative correlations between SPI and both ICRS and Mankin scores were observed ( r = 0.502, P < 0.001, and r = 0.255, P = 0.02, respectively). No correlation was observed between SPI and sGAG content ( r = 0.004, P = 0.973). Conclusions: SPI values may be used as a quantitative means of cartilage evaluation with sufficient reliability among users. Due to the significant learning curve, adequate training should be absolved before routine use of the technique.
The association between rotational thromboelastometry parameters and blood loss after cardiac surgery with cardiopulmonary bypass
Authors: Bosch Yvonne PJ , Weerwind Patrick W , ten Cate Hugo , Henskens Yvonne MC , Nelemans Patty J , Dieri Raed Al , Maessen Jos G , Mochtar Baheramsjah 
Published: August 2015

Background: The objectives of this study were to investigate the changes in routine laboratory tests and rotational thromboelastometry variables after cardiopulmonary bypass and to investigate the association of these variables with postoperative blood loss. Methods: Blood was obtained at baseline, at admission to the intensive care unit, and at the first postoperative day. Rotational thromboelastometry tests and measurements of haemoglobin, haematocrit, platelet count, and plasma fibrinogen level (Clauss method) were performed. Excessive bleeding was defined as >1000 mL of chest tube drainage after surgery within 24 h. Results: After cardiopulmonary bypass, haemotocrit was decreased by 21%. Platelet count and fibrinogen level decreased more (35% and 42%, respectively). The largest change was found for clot formation time of INTEM (intrinsically activated coagulation) (+73%). Platelet count was not reflected by maximal clot firmness of PLATEM (platelet component specific coagulation). The decreases in FIBTEM (fibrinogen specific coagulation) maximum clot firmness and fibrinogen at the intensive care unit admission were comparable. Test results outside the reference range were associated with higher median blood loss when compared to values within the reference range, with most significant association for fibrinogen (p < 0.001) and FIBTEM maximal clot firmness (p = 0.016). At baseline, the ability of rotational thromboelastometry parameters to predict postoperative blood loss >1000 mL is higher than the predictive value of fibrinogen (area under the curve = 0.642; 95% confidence interval: 0.487–0.796), but only areas under the curve of APTEM (extrinsically activated coagulation with exclusion of fibrinolysis component) were significantly higher (p⩽0.05). At intensive care unit admission, the highest area under the curve was 0.689 (95% confidence interval: 0.549–0.828) for FIBTEM maximum clot firmness. Conclusion: Rotational thromboelastometry could be a useful point-of-care method to monitor haemostasis because it is a faster and more informative method, and it has a slightly higher predictive ability for blood loss compared to fibrinogen.
SLAP Repairs With Combined Procedures Have Lower Failure Rate Than Isolated Repairs in a Military Population
Authors: Waterman Brian R. , Arroyo William , Heida Kenneth , Burks Robert , Pallis Mark 
Published: August 2015

Background: Injuries to the superior glenoid labrum represent a significant cause of shoulder pain among active patients. The physical requirements of military service may contribute to an increased risk of injury. Limited data are available regarding the success of superior labral anterior posterior (SLAP) repairs in an active military population. Purpose: To quantify the rate of clinical failure and surgical revision after isolated and combined SLAP repair. Study Design: Cohort study; Level of evidence, 3. Methods: All consecutive active-duty servicemembers undergoing arthroscopic repair of type II SLAP lesions at a single institution between 2006 and 2012 were identified. Patients with less than 2-year clinical follow-up and nonmilitary status were excluded. Demographic variables, surgical variables, and occupational outcomes were extracted from electronic medical records and confirmed with the US Army Physical Disability Agency database. Failure was defined as subsequent revision surgery or medical discharge with persistent shoulder complaints. Results: A total of 192 patients with SLAP repair were identified with a mean follow-up of 50.0 months (SD, 17.0 months). Isolated SLAP repair occurred in 31.3% (n = 60) versus 68.8% (n = 132) with concomitant procedures. At final follow-up, 37.0% (n = 71) of patients reported some subjective activity-related shoulder pain. Postoperative return to duty occurred in 79.6% (n = 153), and only 20.3% (n = 39) were discharged with continuing shoulder disability. The combined rotator cuff repair (96%; P = .023) and anteroinferior labral repair group (88%; P = .056) had a higher rate of functional return than isolated SLAP repair (70%). Thirty-one (16.1%) patients were classified as surgical failure and required revision. Of these, the majority of patients undergoing biceps tenodesis (76%) returned to active duty, as compared with revision SLAP repair (17%). Lower demand occupation and the presence of combined shoulder injuries ( P = .011 and .016, respectively) were significantly associated with a lower risk of medical discharge and revision surgery, respectively. Conclusion: Favorable outcomes can be anticipated in the majority of military servicemembers after arthroscopic SLAP repair, particularly with combined shoulder injuries. Revision surgery occurred in 16% of patients after primary SLAP repair. Clinical Relevance: Isolated repair of unstable SLAP lesions and/or increased upper extremity demands are associated with higher failure rates in this population.
Lack of glucocorticoid-induced leucine zipper (GILZ) deregulates B-cell survival and results in B-cell lymphocytosis in mice
Authors: Bruscoli Stefano , Biagioli Michele , Sorcini Daniele , Frammartino Tiziana , Cimino Monica , Sportoletti Paolo , Mazzon Emanuela , Bereshchenko Oxana , Riccardi Carlo 
Published: August 2015

Key PointsGILZ-deficient mice develop B-cell lymphocytosis. GILZ deficiency precludes GC-mediated B-cell apoptosis.
IL-1β, in contrast to TNFα, is pivotal in blood-induced cartilage damage and is a potential target for therapy
Authors: van Vulpen Lize F. D. , Schutgens Roger E. G. , Coeleveld Katja , Alsema Els C. , Roosendaal Goris , Mastbergen Simon C. , Lafeber Floris P. J. G. 
Published: August 2015

Key PointsBlood-induced joint damage is fully prevented by blocking IL-1β with a monoclonal antibody or receptor antagonist, not by TNFα blockade. IL-1β blockade prevents release of IL-6 but not TNFα from monocyte/macrophages, whereas TNFα blockade does not affect IL-1β or IL-6 release.
Multicenter study of combination DEP regimen as a salvage therapy for adult refractory hemophagocytic lymphohistiocytosis
Authors: Wang Yini , Huang Wenqiu , Hu Liangding , Cen Xinan , Li Lihong , Wang Jijun , Shen Jianliang , Wei Na , Wang Zhao 
Published: August 2015

Key Points
A salvage therapy for adults with refractory hemophagocytic lymphohistiocytosis. Liposomal doxorubicin treatment combined with etoposide and methylprednisolone showed an encouraging overall response and was well-tolerated.
Molecular prediction of durable remission after first-line fludarabine-cyclophosphamide-rituximab in chronic lymphocytic leukemia
Authors: Rossi Davide , Terzi-di-Bergamo Lodovico , De Paoli Lorenzo , Cerri Michaela , Ghilardi Guido , Chiarenza Annalisa , Bulian Pietro , Visco Carlo , Mauro Francesca R. , Morabito Fortunato , Cortelezzi Agostino , Zaja Francesco , Forconi Francesco , Laurenti Luca , Del Giudice Ilaria , Gentile Massimo , Vincelli Iolanda , Motta Marina , Coscia Marta , Rigolin Gian Matteo , Tedeschi Alessandra , Neri Antonino , Marasca Roberto , Perbellini Omar , Moreno Carol , Del Poeta Giovanni , Massaia Massimo , Zinzani Pier Luigi , Montillo Marco , Cuneo Antonio , Gattei Valter , Foà Robin , Gaidano Gianluca 
Published: August 2015

Key Points
CLL patients harboring mutated IGHV genes but neither 11q or 17p deletion experience durable remission after frontline FCR.
Erythroferrone contributes to hepcidin suppression and iron overload in a mouse model of β-thalassemia
Authors: Kautz Léon , Jung Grace , Du Xin , Gabayan Victoria , Chapman Justin , Nasoff Marc , Nemeth Elizabeta , Ganz Tomas 
Published: August 2015

Abstract
Inherited anemias with ineffective erythropoiesis, such as β-thalassemia, manifest inappropriately low hepcidin production and consequent excessive absorption of dietary iron, leading to iron overload. Erythroferrone (ERFE) is an erythroid regulator of hepcidin synthesis and iron homeostasis. Erfe expression was highly increased in the marrow and spleen of HbbTh3/+ mice (Th3/+), a mouse model of thalassemia intermedia. Ablation of Erfe in Th3/+ mice restored normal levels of circulating hepcidin at 6 weeks of age, suggesting ERFE could be a factor suppressing hepcidin production in β-thalassemia. We examined the expression of Erfe and the consequences of its ablation in thalassemic mice from 3 to 12 weeks of age. The loss of ERFE in thalassemic mice led to full restoration of hepcidin mRNA expression at 3 and 6 weeks of age, and significant reduction in liver and spleen iron content at 6 and 12 weeks of age. Ablation of Erfe slightly ameliorated ineffective erythropoiesis, as indicated by reduced spleen index, red cell distribution width, and mean corpuscular volume, but did not improve the anemia. Thus, ERFE mediates hepcidin suppression and contributes to iron overload in a mouse model of β-thalassemia.
Phylogeny and biogeography of Primula sect. Armerina: implications for plant evolution under climate change and the uplift of the Qinghai-Tibet Plateau
Authors: Ren Guangpeng , Conti Elena , Salamin Nicolas 
Published: August 2015

AbstractBackgroundThe historical orogenesis and associated climatic changes of mountain areas have been suggested to partly account for the occurrence of high levels of biodiversity and endemism. However, their effects on dispersal, differentiation and evolution of many groups of plants are still unknown. In this study, we examined the detailed diversification history ofPrimulasect.Armerina, and used biogeographic analysis and macro-evolutionary modeling to investigate a series of different questions concerning the evolution of the geographical and ecological distribution of the species in this section.ResultsWe sequenced five chloroplast and one nuclear genes for species ofPrimulasect.Armerina. Neither chloroplast nor nuclear trees support the monophyly of the section. The major incongruences between the two trees occur among closely related species and may be explained by hybridization. Our dating analyses based on the chloroplast dataset suggest that this section began to diverge from its relatives around 3.55 million years ago, largely coinciding with the last major uplift of the Qinghai-Tibet Plateau (QTP). Biogeographic analysis supports the origin of the section in the Himalayan Mountains and dispersal from the Himalayas to Northeastern QTP, Western QTP and Hengduan Mountains. Furthermore, evolutionary models of ecological niches show that the twoP. fasciculataclades have significantly different climatic niche optima and rates of niche evolution, indicating niche evolution under climatic changes and further providing evidence for explaining their biogeographic patterns.ConclusionOur results support the hypothesis that geologic and climatic events play important roles in driving biological diversification of organisms in the QTP area. The Pliocene uplift of the QTP and following climatic changes most likely promoted both the inter- and intraspecific divergence ofPrimulasect.Armerina.This study also illustrates how niche evolution under climatic changes influences biogeographic patterns.
Molecular phylogeny of Squaliformes and first occurrence of bioluminescence in sharks
Authors: Straube Nicolas , Li Chenhong , Claes Julien M. , Corrigan Shannon , Naylor Gavin J. P. 
Published: August 2015

Abstract
Background
Squaliform sharks represent approximately 27 % of extant shark diversity, comprising more than 130 species with a predominantly deep-dwelling lifestyle. Many Squaliform species are highly specialized, including some that are bioluminescent, a character that is reported exclusively from Squaliform sharks within Chondrichthyes. The interfamiliar relationships within the order are still not satisfactorily resolved. Herein we estimate the phylogenetic interrelationships of a generic level sampling of “squaloid” sharks and closely related taxa using aligned sequences derived from a targeted gene capture approach. The resulting phylogenetic estimate is further used to evaluate the age of first occurrence of bioluminescence in Squaliformes.

Results
Our dataset comprised 172 putative ortholog exon sequences. Phylogenetic estimates result in a fully resolved tree supporting a monophyletic lineage of Squaliformes excluding Echinorhinus. Non-luminous Squalidae are inferred to be the sister to a clade comprising all remaining Squaliform families. Our results suggest that the origin of photophores is coincident with an elevated diversification rate and the splitting of families Dalatiidae, Etmopteridae, Oxynotidae and Somniosidae at the transition of the Lower to the Upper Cretaceous. The presence of luminous organs was confirmed for the Sleeper shark genus Zameus. These results indicate that bioluminescence in sharks is not restricted solely to the families Etmopteridae and Dalatiidae as previously believed.

Conclusions
The sister-clade to non-luminous Squalidae comprises five families. The presence of photophores is reported for extant members of three out of these five families based on results of this study, i.e. Lantern sharks (Etmopteridae), Kitefin sharks (Dalatiidae) and Sleeper sharks (Somniosidae). Our results suggest that the origin of luminous organs arose during the rapid diversification event that gave rise to the extant Squaliform families. These inferences are consistent with the idea of diversification of Squaliform sharks being associated with the emergence of new deep-sea habitats in the Lower Cretaceous, which may have been facilitated by the evolution of bioluminescence.
Prevalence and microbiological characteristics of clinically infected foot‐ulcers in patients with rheumatoid arthritis: a retrospective exploratory study
Authors: Fitzgerald Pauline , Siddle Heidi J. , Backhouse Michael R. , Nelson E. Andrea 
Published: August 2015

AbstractBackgroundThe prevalence of foot ulcers in patients with rheumatoid arthritis (RA) has been reported at almost 10 %. These foot ulcers often occur at multiple sites and are reoccurring, with the potential risk of infection increased due to RA diagnosis and disease modifying medications. The objective of this study was to estimate the prevalence of clinical infection in foot‐ulcers of patients with RA; describe the microbiological characteristics and investigate risk factors.MethodsRetrospective clinical data was collected for all patients attending a rheumatology foot ulcer clinic between 1st May 2012 and 1st May 2013: wound swab data was collected from those with clinical infection.ResultsTwenty‐eight patients with RA and foot‐ulcers were identified; eight of these patients had clinical infection and wound swabs taken (29 %).Of these eight patients there were equal men and women, with median age 74 years, and average disease duration 22 years.Cardiovascular disease/peripheral‐vascular disease (CVD/PVD) were reported in six patients, diabetes in two patients.Six patients were treated with disease‐modifying anti‐rheumatic drugs (DMARDs); three were on biologic medications and two on steroids.Five wound swabs cultured skin flora, one staphylococcus aureus, one had no growth after culture; and one was rejected due to labelling error.ConclusionAlmost a third of people with RA and foot ulcers attending clinic over one year had clinical infection, however microbiological analysis failed to isolate pathogens in six of seven wound swabs. This may be due to inaccurate diagnosis of ulcer infection or to issues with sampling, collection, transport, analysis or reporting. There was insufficient data to relate risk of clinical infection with risk factors.Further research is required to identify the most appropriate techniques for infection diagnosis, wound sampling and processing.Trial registrationEthical approval was obtained from University of Leeds, Faculty of Medicine and Health (Reference number: SHREC/RP/349).
The association between periodontal disease and metabolic syndrome among outpatients with diabetes in Jordan
Authors: Alhabashneh Rola , Khader Yousef , herra Zaid , Asa’ad Farah 
Published: August 2015

Abstract
Background
To date, conflicting results have been reported about the association between metabolic syndrome (MetS) and periodonttitis.

Methods
Two hundred and eighty patients with type 2 diabetes were recruited from outpatients visiting diabetes clinics in Islamic Hospital, Amman-Jordan. The oral hygiene and the periodontal status of all teeth, excluding third molars, were assessed using the plaque index of Silness and Löe, the gingival index of Löe and Silness, probing pocket depth (PPD), and clinical attachment level (CAL). Data were analyzed using the general linear model multivariate procedure with average PPD, average CAL, percent of teeth with CAL ≥3 mm, and percent of teeth with PPD ≥3 mm as outcome variables and diabetes, MetS and its individual components as predictors.

Results
Overall, 83.2 % of patients with diabetes had MetS. In the multivariate analysis, patients with MetS had a significantly more severe periodontitis, as measured by average PPD and average CAL (P < 0.005). The extent of periodontitis, as measured by the percent of teeth with CAL ≥3 mm and the percent of teeth with PPD ≥3 mm, was also significantly greater among patients with MetS (P < 0.005). As the number of metabolic components additional to diabetes increased, the odds of having periodontitis increased, and the odds were greatest when all the components additional to diabetes were present (OR = 10.77, 95 % CI: 2.23 -51.95).

Conclusion
Patients with MetS displayed more severe and extensive periodontitis. Having other MetS components additional to diabetes increased the odds of having periodontitis.
Criminal justice pathways to psychiatric care for
psychosis
Authors: Bhui Kamaldeep , Ullrich Simone , Kallis Constantinos , Coid Jeremy W. 
Published: August 2015

BackgroundSome patients are at higher risk of contact with criminal justice
agencies when experiencing a first episode of psychosis.AimsTo investigate whether violence explains criminal justice pathways (CJPs)
for psychosis in general, and ethnic vulnerability to CJPs.MethodTwo-year population-based survey of people presenting with a
first-episode of psychosis. A total of 481 patients provided information
on pathways to psychiatric care. The main outcome was a CJP at first
contact compared with other services on the care pathway.ResultsCJPs were more common if there was violence at first presentation (odds
ratio (OR) = 4.23, 95% CI 2.74–6.54, P<0.001), drug
use in the previous year (OR = 2.28, 95% CI 1.50–3.48,
P<0.001) and for high psychopathy scores (OR = 2.54,
95% CI 1.43–4.53, P = 0.002). Compared with White
British, CJPs were more common among Black Caribbean (OR = 2.97, 95% CI
1.54–5.72, P<0.001) and Black African patients (OR =
1.95, 95% CI 1.02–3.72, P = 0.01). Violence mediated
30.2% of the association for Black Caribbeans, but was not a mediator for
Black African patients. These findings were sustained after adjustment
for age, marital status, gender and employment.ConclusionsCJPs were more common in violent presentations, for greater psychopathy
levels and drug use. Violence presentations did not fully explain ethnic
vulnerability to CJPs.
E4-Ubiquitin ligase Ufd2 stabilizes Yap8 and modulates arsenic stress responses independent of the U-box motif
Authors: Ferreira Rita T. , Menezes Regina A. , Rodrigues-Pousada Claudina 
Published: August 2015

ABSTRACT
Adaptation of Saccharomyces cerevisiae cells to arsenic stress is mediated through the activation of arsenic detoxification machinery by the Yap8 transcription factor. Yap8 is targeted by the ubiquitin proteasome system for degradation under physiological conditions, yet it escapes proteolysis in arsenic-injured cells by a mechanism that remains to be elucidated. Here, we show that Ufd2, an E4-Ubiquitin (Ub) ligase, is upregulated by arsenic compounds both at mRNA and protein levels. Under these conditions, Ufd2 interacts with Yap8 mediating its stabilization, thereby controlling expression of ACR3 and capacity of cells to adapt to arsenic injury. We also show that Ufd2 U-box domain, which is associated to the ubiquitination activity of specific ubiquitin ligases, is dispensable for Yap8 stability and has no role in cell tolerance to arsenic stress. Thus, our data disclose a novel Ufd2 role beyond degradation. This finding is further supported by genetic analyses showing that proteins belonging to Ufd2 proteolytic pathways, namely Ubc4, Rad23 and Dsk2, mediate Yap8 degradation.