bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation of ignimbrites using characteristic remanent magnetization and anisotropy of magnetic susceptibility, Central Andes, Bolivia
Authors: Ort Michael H. , de Silva Shanaka L. , Jiménez C. Néstor , Jicha Brian R. , Singer Bradley S. 
Published: December 2012

Large ignimbrite flare‐ups provide records of profound crustal modification during batholith formation at depth. The locations of source calderas and volumes and ages of the eruptions must be determined to develop models for the tectonomagmatic processes that occur during these events. Although high‐precision isotopic ages of the ignimbrites are critical, less expensive and more rapid techniques, such as paleomagnetism, can extend the temporal information from dated outcrops. Paleomagnetic and rock magnetic data, including characteristic remanent magnetization (ChRM) and anisotropy of magnetic susceptibility (AMS), from the Altiplano‐Puna Volcanic Complex of the Central Andes reliably identify calderas and eight associated Mio‐Pliocene ignimbrites.ChRM results indicate a larger between‐site error for most ignimbrites, in comparison to within‐site scatter. Part of this dispersion may be due to tumescence/detumescence associated with the caldera‐forming eruptions, but most of the effect is probably due to the recording of paleosecular variation during cooling and vapor‐phase crystallization of the thick ignimbrites. AMS data identify the source calderas for four ignimbrites and provide limits on possible post‐emplacement rotations of the deposits. AMS data indicate significant topographic control on inferred flow directions, implying that the flows were dense and/or of low mobility.
Cryopreservation of Sperm of an Indigenous Endangered Fish, Pabda Catfish Ompok pabda
Authors: Sarder M Rafiqul I , Saha Shankar K , Sarker M Fazle M 
Published: December 2012

Abstract
This study focused on the development of a protocol for cryopreservation of sperm of the endangered fish species, Pabda Catfish Ompok pabda. The activation of sperm motility was tested at various osmolalities of NaCl. The motility of sperm decreased with the increase of osmolality; it was completely inhibited at 319 mOsmol/kg. Toxicity of cryoprotectants to sperm was evaluated using dimethyl sulfoxide (DMSO), methanol, and ethanol at 5, 10, and 15% concentrations at an incubation time of 0–35 min. Five percent and 10% cryoprotectants produced 45–75% and 40–75% motility for the 5- and 10-min incubation times, respectively. Sperm incubated with 15% cryoprotectant had less motility from the beginning of incubation. The cryoprotectant was toxic to sperm. Three extenders, Alsever's solution, egg-yolk citrate, and urea egg-yolk, as well as three cryoprotectants, DMSO, methanol, and ethanol, were used for the preservation of sperm. Alsever's solution with DMSO showed best performance producing highest equilibration motility (83 ± 3.3%, mean ± SE) and postthaw motility (71 ± 4.4%) followed by levels of 68 ± 1.7% and 51 ± 1.7%, respectively, with Alsever's solution plus methanol. Sperm preserved with Alsever's solution plus DMSO produced 79.33% fertilization and 42.38% hatching while fresh sperm yielded 82% fertilization and 53.49% hatching. No statistical comparisons between cryopreserved and fresh sperm were made as we did not standardize the sperm concentration for fresh and cryopreserved sperm during breeding. Fry produced from both cryopreserved and fresh sperm were reared in aquaria for 6 weeks and no significant differences for length (P = 0.452) and weight (P = 0.431) were observed between the two groups. The protocols developed through this study can be applied for conservation of endangered Pabda Catfish, and the findings of breeding and growth studies suggest it would be useful to disseminate the cryopreservation technology to commercial hatcheries.
Growth and Acute Temperature Tolerance of June Sucker Juveniles Fed Varying Dietary Protein and Lipid Levels With and Without Supplemental Dicalcium Phosphate
Authors: Sealey Wendy M , Gaylord T Gibson , Toner Matt , Ilgen Jason , Fraser W C , Hooley Christopher G , Barrows Frederic T 
Published: December 2012

Abstract
Reports from hatcheries have demonstrated that commercially available diets are inadequate for June Suckers Chasmistes liorus and suggest that fish quality can be improved through species-specific diet optimization. To determine dietary protein and lipid levels for juvenile June Suckers, practical-type diets were formulated with 35, 40, or 45% protein and 8, 12, or 16% lipid and tested in a 3 × 3 factorial design. Two additional diets (45% protein and 16% lipid [45:16] without dicalcium phosphate supplementation and 45:16 with 2× dicalcium phosphate) were formulated to test the necessity of dicalcium phosphate supplementation. Each diet was fed to three replicate tanks (average initial fish weight ± SD = 4.48 ± 0.24 g) of fish per treatment (85 fish/tank); only duplicate tanks were used for the nondicalcium phosphate-supplemented treatment. Fish were reared in 80-L tanks supplied with 4 L/min of 22°C spring water and fed 6–4% body weight (BW)/d by automated belt feeders, 6 d/week. At the end of 12 weeks of culture, tissue samples were obtained to determine nutrient partitioning. An acute temperature challenge was also conducted to investigate dietary adequacy. Significant effects of diet on growth and temperature tolerance were observed. Fish fed the diet with 45% crude protein gained more weight than did fish fed 40% or 35% crude protein, and fish fed 16% dietary lipid gained more weight than did fish fed 12% and 8% crude lipid. However, the composition of this growth indicated that fish fed the 16% lipid stored larger amounts of lipid in both the visceral cavity and the muscle. A significant negative interactive effect of dietary protein and lipid on temperature tolerance was observed at 45% crude protein and 16% lipid. Results of this study define acceptable ranges of dietary protein and lipid for juvenile June Suckers and provide culturists information to make better choices regarding appropriate diets for this species.
A corpus-based deconstructive strategy for critically engaging with arguments
Authors: O'Halloran Kieran 
Published: December 2012

My broad concern is with texts which aim to persuade an audience of a particular point of view on a particular topic – persuasion texts. Political speeches and newspaper editorials are examples of this text type. I put forward a strategy for critically engaging with such texts. Its focus is a persuasion text's cohesion – how it is held together through its vocabulary and grammar. The strategy explores whether or not the cohesion of a persuasion text is unstable, if it deconstructs. Since a persuasion text's credibility is dependent, amongst other things, on effective cohesion, showing where a persuasion text's cohesion deconstructs diminishes its credibility. I call this critical reading strategy Electronic Deconstruction: ‘Electronic’ reflects the fact that the strategy draws on corpus linguistic method; ‘Deconstruction’ refers to the deconstructive approach of this strategy. An advantage of Electronic Deconstruction is that it can still facilitate critical engagement with an argument where it is difficult to reconstruct all its premises. This is because its evaluative focus is a persuasion text's cohesive structure rather than its logical structure. To demonstrate Electronic Deconstruction, the article employs a case-study, a text written in 2008 by the late political journalist, Christopher Hitchens, which justified his continuing support for the 2003 Iraq intervention by US-led coalition forces. After highlighting a number of frustrations in identification of its arguments, and thus for critical assessment of its logical soundness, I show how Electronic Deconstruction as an alternative critical engagement circumvents incomplete reconstruction by doing the following: revealing that the text's cohesive structure is unstable via an electronic deconstructive analysis which draws on a two-billion word corpus, the Oxford English Corpus. The article employs two corpus linguistic software tools: ‘Sketchengine’ ( http://www.sketchengine.co.uk/ ) and ‘WMatrix’ ( http://ucrel.lancs.ac.uk/wmatrix/ ).
Silica final lens performance in laser fusion facilities: HiPER and LIFE
Authors: Garoz D. , González-Arrabal R. , Juárez R. , Álvarez J. , Sanz J. , Perlado J.M. , Rivera A. 
Published: December 2012

Abstract
Nowadays, the projects LIFE (Laser Inertial Fusion Energy) in USA and HiPER (High Power Laser Energy Research) in Europe are the most advanced ones to demonstrate laser fusion energy viability. One of the main points of concern to properly achieve ignition is the performance of the final optics (lenses) under the severe irradiation conditions that take place in fusion facilities. In this paper, we calculate the radiation fluxes and doses as well as the radiation-induced temperature enhancement and colour centre formation in final lenses assuming realistic geometrical configurations for HiPER and LIFE. On these bases, the mechanical stresses generated by the established temperature gradients are evaluated showing that from a mechanical point of view lenses only fulfil specifications if ions resulting from the imploding target are mitigated. The absorption coefficient of the lenses is calculated during reactor startup and steady-state operation. The obtained results reveal the necessity of new solutions to tackle ignition problems during the startup process for HiPER. Finally, we evaluate the effect of temperature gradients on focal length changes and lens surface deformations. In summary, we discuss the capabilities and weak points of silica lenses and propose alternatives to overcome predictable problems.
Electron-cyclotron-current-drive efficiency in DEMO plasmas
Authors: Poli E. , Tardini G. , Zohm H. , Fable E. , Farina D. , Figini L. , Marushchenko N.B. , Porte L. 
Published: December 2012

Abstract
The achievable efficiency for external current drive through electron-cyclotron waves in a demonstration tokamak reactor is investigated. Two possible reactor designs, one for steady state and one for pulsed operation, are considered. Beam propagation, absorption and current drive are modelled employing the beam-tracing technique and including momentum conservation in electron–electron collisions. It is found that for midplane injection the achievable current drive efficiency is limited by second-harmonic absorption at levels consistent with previous studies. Higher efficiencies can be achieved by injecting the beams from the top of the machine, exploiting wave absorption by more energetic (less collisional) electrons. Current drive efficiencies competitive with those usually obtained by neutral beam current drive are reported. These optimum efficiencies are found for frequencies around 230 GHz and 290 GHz for the steady-state and the pulsed DEMO, supposed to operate at a magnetic field B = 5.84 T and B = 7.45 T, respectively.
Impact of adaptive servo‐ventilation on cardiovascular function and prognosis in heart failure patients with preserved left ventricular ejection fraction and sleep‐disordered breathing
Authors: Yoshihisa Akiomi , Suzuki Satoshi , Yamaki Takayoshi , Sugimoto Koichi , Kunii Hiroyuki , Nakazato Kazuhiko , Suzuki Hitoshi , Saitoh Shu‐ichi , Takeishi Yasuchika 
Published: December 2012

AimsEffective pharmacotherapy for heart failure with preserved left ventricular ejection fraction (HFpEF) is still unclear. Sleep‐disordered breathing (SDB) causes cardiovascular dysfunction, giving rise to factors involved in HFpEF. However, it remains unclear whether adaptive servo‐ventilation (ASV) improves cardiovascular function and long‐term prognosis of patients with HFpEF and SDB.Methods and resultsThirty‐six patients with HFpEF (LVEF >50%) and moderate to severe SDB (apnoea–hypopnoea index >15/h) were enrolled. Study subjects (LVEF 56.0%, apnoea–hypopnoea index 36.5/h) were randomly assigned to two groups: 18 patients treated with medications and ASV (ASV group) and 18 patients not treated with ASV (non‐ASV group). NYHA class, cardiac function including LVEF, left atrial volume index (LAVI), E/E', vascular function including flow‐mediated dilatation (FMD) and cardio‐ankle vascular index (CAVI), and levels of BNP and troponin T were determined at baseline and 6 months later. Patients were followed to register cardiac events after enrolment (follow‐up 543 days). ASV therapy improved cardiac diastolic function and decreased CAVI and BNP (NYHA class, 2.3 to 1.5; LAVI, 48.6 to 42.6 mL/m2; E/E', 12.8 to 7.1; CAVI, 9.0 to 7.7; BNP, 121.5 to 58.1 pg/mL, P < 0.0125, respectively). LVEF, FMD, and troponin T did not change significantly in either group. Importantly, the event‐free rate was significantly higher in the ASV group than in the non‐ASV group (94.4% vs. 61.1%, log‐rank P < 0.05).ConclusionASV may improve the prognosis of HFpEF patients with SDB, with favourable effects such as improvement of symptoms, cardiac diastolic function, and arterial stiffness. ASV may be a useful therapeutic tool for HFpEF patients with SDB.
Effects of Propofol, Sevoflurane, Remifentanil, and (S)-Ketamine in Subanesthetic Concentrations on Visceral and Somatosensory Pain–evoked Potentials
Authors: Untergehrer Gisela , Jordan Denis , Eyl Sebastian , Schneider Gerhard 
Published: December 2012

Abstract

Background:
Although electroencephalographic parameters and auditory evoked potentials (AEP) reflect the hypnotic component of anesthesia, there is currently no specific and mechanism-based monitoring tool for anesthesia-induced blockade of nociceptive inputs. The aim of this study was to assess visceral pain–evoked potentials (VPEP) and contact heat–evoked potentials (CHEP) as electroencephalographic indicators of drug-induced changes of visceral and somatosensory pain. Additionally, AEP and electroencephalographic permutation entropy were used to evaluate sedative components of the applied drugs.

Methods:
In a study enrolling 60 volunteers, VPEP, CHEP (amplitude N2-P1), and AEP (latency Nb, amplitude Pa-Nb) were recorded without drug application and at two subanesthetic concentration levels of propofol, sevoflurane, remifentanil, or (s)-ketamine. Drug-induced changes of evoked potentials were analyzed. VPEP were generated by electric stimuli using bipolar electrodes positioned in the distal esophagus. For CHEP, heat pulses were given to the medial aspect of the right forearm using a CHEP stimulator. In addition to AEP, electroencephalographic permutation entropy was used to indicate level of sedation.

Results:
With increasing concentrations of propofol, sevoflurane, remifentanil, and (s)-ketamine, VPEP and CHEP N2-P1 amplitudes decreased. AEP and electroencephalographic permutation entropy showed neither clinically relevant nor statistically significant suppression of cortical activity during drug application.

Conclusions:
Decreasing VPEP and CHEP amplitudes under subanesthetic concentrations of propofol, sevoflurane, remifentanil, and (s)-ketamine indicate suppressive drug effects. These effects seem to be specific for analgesia.
Reciprocal Interaction of the Circadian Clock with the Iron Homeostasis Network in Arabidopsis
Authors: Hong Sunghyun , Kim Sun A. , Guerinot Mary Lou , McClung C. Robertson 
Published: December 2012

Abstract
In plants, iron (Fe) uptake and homeostasis are critical for survival, and these processes are tightly regulated at the transcriptional and posttranscriptional levels. Circadian clocks are endogenous oscillating mechanisms that allow an organism to anticipate environmental changes to coordinate biological processes both with one another and with the environmental day/night cycle. The plant circadian clock controls many physiological processes through rhythmic expression of transcripts. In this study, we examined the expression of three Fe homeostasis genes (IRON REGULATED TRANSPORTER1 [IRT1], BASIC HELIX LOOP HELIX39, and FERRITIN1) in Arabidopsis (Arabidopsis thaliana) using promoter:LUCIFERASE transgenic lines. Each of these promoters showed circadian regulation of transcription. The circadian clock monitors a number of clock outputs and uses these outputs as inputs to modulate clock function. We show that this is also true for Fe status. Fe deficiency results in a lengthened circadian period. We interrogated mutants impaired in the Fe homeostasis response, including irt1-1, which lacks the major high-affinity Fe transporter, and fit-2, which lacks Fe deficiency-induced TRANSCRIPTION FACTOR1, a basic helix-loop-helix transcription factor necessary for induction of the Fe deficiency response. Both mutants exhibit symptoms of Fe deficiency, including lengthened circadian period. To determine which components are involved in this cross talk between the circadian and Fe homeostasis networks, we tested clock- or Fe homeostasis-related mutants. Mutants defective in specific clock gene components were resistant to the change in period length under different Fe conditions observed in the wild type, suggesting that these mutants are impaired in cross talk between Fe homeostasis and the circadian clock.
Alteration of the Interconversion of Pyruvate and Malate in the Plastid or Cytosol of Ripening Tomato Fruit Invokes Diverse Consequences on Sugar But Similar Effects on Cellular Organic Acid, Metabolism, and Transitory Starch Accumulation
Authors: Osorio Sonia , Vallarino José G. , Szecowka Marek , Ufaz Shai , Tzin Vered , Angelovici Ruthie , Galili Gad , Fernie Alisdair R. 
Published: December 2012

Abstract
The aim of this work was to investigate the effect of decreased cytosolic phosphoenolpyruvate carboxykinase (PEPCK) and plastidic NADP-dependent malic enzyme (ME) on tomato (Solanum lycopersicum) ripening. Transgenic tomato plants with strongly reduced levels of PEPCK and plastidic NADP-ME were generated by RNA interference gene silencing under the control of a ripening-specific E8 promoter. While these genetic modifications had relatively little effect on the total fruit yield and size, they had strong effects on fruit metabolism. Both transformants were characterized by lower levels of starch at breaker stage. Analysis of the activation state of ADP-glucose pyrophosphorylase correlated with the decrease of starch in both transformants, which suggests that it is due to an altered cellular redox status. Moreover, metabolic profiling and feeding experiments involving positionally labeled glucoses of fruits lacking in plastidic NADP-ME and cytosolic PEPCK activities revealed differential changes in overall respiration rates and tricarboxylic acid (TCA) cycle flux. Inactivation of cytosolic PEPCK affected the respiration rate, which suggests that an excess of oxaloacetate is converted to aspartate and reintroduced in the TCA cycle via 2-oxoglutarate/glutamate. On the other hand, the plastidic NADP-ME antisense lines were characterized by no changes in respiration rates and TCA cycle flux, which together with increases of pyruvate kinase and phosphoenolpyruvate carboxylase activities indicate that pyruvate is supplied through these enzymes to the TCA cycle. These results are discussed in the context of current models of the importance of malate during tomato fruit ripening.
Multiple Roles for UV RESISTANCE LOCUS8 in Regulating Gene Expression and Metabolite Accumulation in Arabidopsis under Solar Ultraviolet Radiation
Authors: Morales Luis O. , Brosché Mikael , Vainonen Julia , Jenkins Gareth I. , Wargent Jason J. , Sipari Nina , Strid Åke , Lindfors Anders V. , Tegelberg Riitta , Aphalo Pedro J. 
Published: December 2012

Abstract
Photomorphogenic responses triggered by low fluence rates of ultraviolet B radiation (UV-B; 280–315 nm) are mediated by the UV-B photoreceptor UV RESISTANCE LOCUS8 (UVR8). Beyond our understanding of the molecular mechanisms of UV-B perception by UVR8, there is still limited information on how the UVR8 pathway functions under natural sunlight. Here, wild-type Arabidopsis (Arabidopsis thaliana) and the uvr8-2 mutant were used in an experiment outdoors where UV-A (315–400 nm) and UV-B irradiances were attenuated using plastic films. Gene expression, PYRIDOXINE BIOSYNTHESIS1 (PDX1) accumulation, and leaf metabolite signatures were analyzed. The results show that UVR8 is required for transcript accumulation of genes involved in UV protection, oxidative stress, hormone signal transduction, and defense against herbivores under solar UV. Under natural UV-A irradiance, UVR8 is likely to interact with UV-A/blue light signaling pathways to moderate UV-B-driven transcript and PDX1 accumulation. UVR8 both positively and negatively affects UV-A-regulated gene expression and metabolite accumulation but is required for the UV-B induction of phenolics. Moreover, UVR8-dependent UV-B acclimation during the early stages of plant development may enhance normal growth under long-term exposure to solar UV.
Respiratory Syncytial Virus Regulates Human MicroRNAs by Using Mechanisms Involving Beta Interferon and NF-κB
Authors: Thornburg Natalie J. , Hayward Sarah L. , Crowe James E. 
Published: December 2012

ABSTRACTRespiratory syncytial virus (RSV) is the most common viral cause of severe lower respiratory tract illness in infants and children. The virus replicates in polarized epithelial cells in the airway and, to a lesser extent, infects airway antigen-presenting cells, such as dendritic cells (DCs). RSV possesses a number of expressed genes that antagonize the effect of type I interferons and other related host factor pathways that inhibit replication efficiency. Virus infection alters host gene transcription and the translation of host transcripts through specific antagonism of the function of host proteins, through induction of RNA stress granules, and through induction of altered patterns of host gene expression. In healthy cells, microRNAs (miRNAs) regulate gene expression by targeting the noncoding region of mRNA molecules to cause silencing or degradation of transcripts. It is not known whether or not RSV infection alters the level of microRNAs in cells. We profiled the pattern of expression of host cell microRNAs in RSV-infected epithelial cells or DCs and found that RSV did alter microRNA expression but in a cell-type-specific manner. The studies showed that let-7b was upregulated in DCs, while let-7i and miR-30b were upregulated in epithelial cells in a process that required viral replication. Interestingly, we found that the RSV nonstructural genes NS1 and NS2 antagonized the upregulation of let-7i and miR-30b. RSV appears to manipulate host cell gene expression through regulation of expression of miRNAs related to the interferon response. The data suggest a new mechanism of virus-host cell interactions for paramyxoviruses.IMPORTANCERespiratory syncytial virus (RSV) is the most common cause of serious lower respiratory tract illness in infants and children. The human innate immune response inhibits RSV replication early after inoculation, principally through the effect of substances called interferons. The virus, however, has developed several mechanisms for counteracting the host innate immune response. It is not known whether or not RSV infection alters the expression of host microRNAs, which are short RNA sequences that are posttranscriptional regulators. This paper shows that RSV does induce unique patterns of microRNA expression related to the NF-κB pathway or interferon pathways. The microRNA profiles differed depending on the cell type that was infected, airway cell or antigen-presenting cell. Interestingly, the virus appears to counteract the microRNA response by expressing nonstructural viral genes in the cell that reduce microRNA induction. The data suggest a new way in which paramyxoviruses regulate the host cell response to infection.