bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract A110: Associations between genetic variations in inflammation and innate immunity pathways and colorectal cancer
Authors: Wang Hansong , Taverna Darin , Stram Daniel O. , Cheng Iona , Wilkens Lynne R. , Burnett Terrilea , Newcomb Polly A. , Kolonel Laurence N. , Henderson Brian E. , Duggan David , Ulrich Cornelia M. , Marchand Loic Le 
Published: January 2014

Abstract
A strong association between chronic inflammation and colorectal cancer (CRC) risk has been observed. We postulated that common variation in genes involved in the inflammation and innate immunity pathways may result in a chronic pro-inflammatory state that may lead to increased CRC risk. We comprehensively examined the associations of 724 tagSNPs in 37 key genes in these pathways with CRC in two large well-characterized studies. The screening phase included 2,322 discordant sibships (2,535 cases, 3,915 unaffected sibling controls) from the Colon Cancer Family Registry. Nineteen gene regions spanned by 99 top hits from main-effect or interaction-effect analyses in phase 1 were followed up by genotyping 351 well-chosen tagSNPs in 4,783 subjects (2,153 cases, 2,630 controls) in five ethnic groups in the Multiethnic Cohort Study. One SNP rs9858822 in PPAR gamma gene was statistically significantly associated with CRC in the replication phase (p=1.5 × 10-4, odds ratio per allele = 1.36), based on a conservative Bonferroni-corrected significance level 0.05 adjusted for the “effective” number of markers (n=306). The risk allele C was common (frequency 0.3) in African Americans but was rare (frequency &lt; 0.05) in Whites, Japanese Americans, Latinos and Native Hawaiians. We did not detect effect modification by race/ethnicity, BMI, or use of aspirin. Strong, although not statistically significant, associations were also observed for SNPs in the neighborhood region of rs9858822 (p-values as low as 2.5 × 10-4). Further replication is needed.
Citation Format: Hansong Wang, Darin Taverna, Daniel O. Stram, Iona Cheng, Lynne R. Wilkens, Terrilea Burnett, Polly A. Newcomb, Laurence N. Kolonel, Brian E. Henderson, David Duggan, Cornelia M. Ulrich, Loic Le Marchand. Associations between genetic variations in inflammation and innate immunity pathways and colorectal cancer. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A110.
Abstract A111: The Awareness on Loop Electrosurgical Excision Procedure as a strategy for reducing the Incidence and Mortality of Cervical Cancer among Nigerian Market Women
Authors: Adetule Olubunmi Olabisi 
Published: January 2014

Abstract
This paper highlights the level of awareness of Loop Electrosurgical Excision Procedure (LEEP) as a strategy for reducing the incidence and mortality of cervical cancer among the reproductive women in Ibadan North Government of Oyo state, Nigeria. A non-experimental descriptive design was used to find out the level of awareness of LEEP among the market women in Ibadan North Local Government of Oyo State. The sampling technique adopted in the study was the simple random sampling. The sample consisted of young adults, adults and aged women including literate and illiterate. The respondents were reassured of the confidentiality of the data. The instrument used basically was self-structured questionnaire. These questionnaires were administered in four major markets which are Bodija, Sango, Gate, and Gbagi markets in Ibadan North Local Government area of Oyo State, Nigeria. On the respondent awareness of LEEP before this study, only 26 (26%) of the respondents strongly agreed that they have heard about LEEP while 15 (15%) were undecided and 59 (59%) said they have never heard about it, which means that majority of the respondents were not aware of LEEP. The women were asked if LEEP is curative and prevent reoccurrence of cervical cancer; 40 (40%) agreed while 33 (33%) were undecided about this question and 27 (27%) disagreed that it is not curative and cervical cancer can still occur. Regarding the necessity for all women to be aware of the LEEP, 84 (84%) agreed that it is important and necessary for all reproductive and menopausal women to be aware of LEEP which will help to reduce the maternal mortality and morbidity rate, while 15 (15%) were undecided and 1 (1%) disagreed. The women were asked if the awareness of LEEP will decrease the maternal mortality rate of cervical cancer. 85 (85%) agreed that awareness of LEEP will decrease the maternal mortality rate which means that if women are aware of LEEP, it will help them to come to the nearby health center for prompt treatment on time,14 (14%) are undecided and only 1(1%) disagreed. The study shows that most Nigerian market women are not aware of LEEP. There is a need for governments and policy makers to increase publicity on the benefits of LEEP so as to reduce the incidence and mortality rate of cervical cancer in Nigeria.
Citation Format: Olubunmi Olabisi Adetule. The awareness on loop electrosurgical excision procedure as a strategy for reducing the incidence and mortality of cervical cancer among Nigerian market women. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A111.
Abstract A116: Inter-scan reproducibility of mammographic density measurements
Authors: Lokate Mariëtte , Emaus Marleen , Kallenberg Michiel , Bakker Marije , Karssemeijer Nico , Veldhuis Wouter , Peeters Petra , Gils Carla van 
Published: January 2014

Abstract
Introduction: A decrease in mammographic density is increasingly used as an intermediate marker to evaluate the effect of interventions for reducing breast cancer risk. Technical aspects, such as a different amount of compression, could influence density measurements. We investigated the inter-scan reproducibility of mammographic density measurements to see whether this may influence measurements of true changes in density.
Methods: We included women with two mammograms made closely after one another (median 12 days), with no true changes in breast density expected to happen: 106 had two Full-Field Digital Mammograms (FFDM), 12 had two Film-Screen Mammograms (FSM) and 52 had one FFDM and one FSM. We estimated intraclass correlation coefficients, and mean absolute density differences between both mammograms.
Results: The intraclass correlation between percent density measurements on two FFDMs was 0.93 (95%CI 0.91-0.95), between two FSMs 0.94 (95%CI 0.84-0.98), and between FFDM and FSM 0.72 (95%CI 0.61-0.80). The mean absolute difference in percent density between two FFDMs was 4.3% (95%CI 3.8-4.9) and between two FSMs 6.5% (95%CI 3.4-9.6). On average, percent density in the same woman was higher on FFDM than on FSM (difference 12.9% (95%CI 11.0-14.8)).
Conclusion: When studying changes in mammographic density, it is important to compare two mammograms of the same type (FSM or FFDM). Even then, although intraclass correlation coefficients are high, the absolute differences in percent density are still of a magnitude that may influence detectability of true changes in mammographic density.
Citation Format: Mariëtte Lokate, Marleen Emaus, Michiel Kallenberg, Marije Bakker, Nico Karssemeijer, Wouter Veldhuis, Petra Peeters, Carla van Gils. Inter-scan reproducibility of mammographic density measurements. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A116.
Abstract A19: miR-19a modulates chemoradiotherapy resistance in human cervical cancer
Authors: Liu Mei , Wu Lingying , Xu Ningzhi , An Jusheng , Hu Jinlong , Huang Manni , Tu Binbin , Hu Chenfei , Wang Zaozao , Zhu Hongxia 
Published: January 2014

Abstract
Cervical cancer is one of the most common cancers in women worldwide. Advanced uterine cervical squamous cell carcinomasuterine (UCSCC) patients in FIGO stage IIb or IIIb treated with platinum based concomitant chemoradiotherapy. However, clinical outcomes vary significantly and are difficult to predict. Tumor intrinsic chemoradiotherapy sensitivity is one of the crucial reasons for concomitant chemoradiotherapy failure in UCSCC. In this study, we identified the relationship between miR-19a and sensitivity to concomitant chemoradiotherapy. The UCSCC patients in FIGO stage IIb or IIIb treated with platinum based concurrent chemoradiotherapy at the Cancer Institute and Hospital, Chinese Academy of Medical Sciences. Patients with progressive disease during first line platinum based chemotherapy or those who suffered recurrent disease within 6 months of completing first line therapy were termed resistant. Patients without recurrence or with recurrences beyond 6 months were termed sensitive. We examined the expression level of miR-19a in 30 cervix biopsies samples, including 10 resistant and 20 sensitive samples. We found miR-19a was significantly up-regulated in resistant group compared with sensitive group (p &lt;0.05). Our study indicated that miR-19a might be a marker to identify cervical cancer patients who are likely to fail concomitant chemoradiotherapy. To verify role of miR-19ain cervical cancer cells is worthy of further investigation.
Citation Format: Mei Liu, Lingying Wu, Ningzhi Xu, Jusheng An, Jinlong Hu, Manni Huang, Binbin Tu, Chenfei Hu, Zaozao Wang, Hongxia Zhu. miR-19a modulates chemoradiotherapy resistance in human cervical cancer. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A19.
Abstract A23: Gene set enrichment analysis of renal cell carcinoma genome-wide association data identifies the JAK-STAT pathway mediating susceptibility
Authors: Shu Xiang , Chen Meng , Ye Yuanqing , Huang Maosheng , Gu Jian , Wood Christopher G. , Wu Xifeng 
Published: January 2014

Abstract
Genome-wide association studies (GWAS) have successfully discovered novel loci associated with renal cell carcinoma (RCC) risk. However, a portion of genetic variation contributing to RCC susceptibility remains uncovered by traditional individual SNP analysis in GWAS. Several pathway-based analytical tools have been developed to complement individual SNP analysis. In this study, we performed gene set enrichment analysis using our RCC GWAS dataset comprised of 894 cases and 1,516 controls with three different tools: GenGen, SNP ratio test, and ALIGATOR. Seven pathways defined by KEGG, GO, and REACTOME were significantly associated with RCC susceptibility in all the three algorithms, with JAK-STAT-signaling (155 genes, PGenGen=0.033, PSNP ratio=0.002, and PALIGATOR=0.002) and Glycine-Serine-Threonine-metabolism (31 genes, PGenGen=0.04, PSNP ratio=0.043, and PALIGATOR&lt;0.001) being the most significant. Gene-based analysis identified 13 genes in the JAK-STAT signaling pathway as being significantly associated with RCC risk, with OSM (oncostatin M) reaching the highest significance (p=0.003). Similar analysis in the Glycine -Serine-Threonine-metabolism pathway identified GLYCTK (glycerate kinase) as the most significant (p=0.003) among seven significant genes identified. We further validated the top significant variants from these two pathways in a replication GWAS dataset of 1,311 cases and 3,423 controls from NCI. One SNP (rs757903) located in PIK3CG (catalytic subunit of PI3K) in the JAK-STAT-signaling pathway was successfully validated with another three reaching borderline significance. Meta-analyses confirmed that these four SNPs were significantly associated with RCC risk, and increased the risk up to 20%. Individuals who carry three to four risk genotypes were at a 2-fold increase in RCC risk, compared to reference group with at most one risk genotype (OR: 1.73, 95% CI: 1.38–2.16, p= 1.47×10−6). Our results suggest that JAK-STAT-signaling may play an important role in RCC tumorigenesis and identify several new avenues for future investigation into the genetic factors mediating susceptibility to RCC.
Citation Format: Xiang Shu, Meng Chen, Yuanqing Ye, Maosheng Huang, Jian Gu, Christopher G. Wood, Xifeng Wu. Gene set enrichment analysis of renal cell carcinoma genome-wide association data identifies the JAK-STAT pathway mediating susceptibility. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A23.
Abstract A24: IGF2/H19 and PEG1/MEST DNA methylation in CIN1, CIN2, CIN3 and cervical cancer in Tanzanian women: In search of disease progression biomarkers
Authors: Vidal Adriana C. , Nye Monica , Murphy Susan K. , Overcash Francine , Oneko Olola , Bartlett John A. , Huang Zhiqing , Mlay Pendo , Obure Joseph , Smith Jennifer S. , Hernandez Brenda , Hoyo Cathrine 
Published: January 2014

Abstract
Background: Epigenetic mechanisms have been hypothesized to be etiologically involved in progression of cervical intraepithelial neoplasia (CIN) to invasive cervical cancer (ICC), although empirical data are lacking.
Methods: We enrolled 148 controls, 38 CIN and 48 ICC cases at Kilimanjaro Christian Medical Centre from 2008-2009. HPV was genotyped by HPV linear array and HIV-1 serostatus was tested by two rapid HIV tests. DNA methylation was measured at differentially methylated regions (DMRs) regulating genomic imprinting of IGF2/H19 and PEG1/MEST imprinted domains, using pyrosequencing. Logistic regression models were used to estimate odd ratios (ORs).
Results: After adjusting for age, HPV infection, parity, hormonal contraceptive use, and HIV-1 serostatus, a 10% decrease in methylation at the IGF2 CTCF3 DMR was associated with a significantly higher risk of ICC (OR=2.00, 95% CI 1.14-3.44) and CIN (OR=1.51, 95% CI 1.00-2.50). We observed similar, albeit weaker pattern of associations between the H19 DMR and ICC and PEG1/MEST (OR= 1.51, 95% CI 0.90-2.53, and OR= 1.44, 95% CI 0.90-2.35, respectively). Restricting analyses to older women (&gt;30 years old) further strengthened these associations.
Conclusions: Aberrant DNA methylation at regulatory regions of imprinted genes may increase susceptibility to CIN and ICC and this association appears independent of HPV infection. While the small sample size limits inference, these findings support the hypothesis that DNA methylation at imprinted domains, including IGF2/H19 and PEG1/MEST, may represent susceptibility loci that can be exploited to identify, from among a large number of CIN, those likely to progress.
Citation Format: Adriana C. Vidal, Monica Nye, Susan K. Murphy, Francine Overcash, Olola Oneko, John A. Bartlett, Zhiqing Huang, Pendo Mlay, Joseph Obure, Jennifer S. Smith, Brenda Hernandez, Cathrine Hoyo. IGF2/H19 and PEG1/MEST DNA methylation in CIN1, CIN2, CIN3 and cervical cancer in Tanzanian women: In search of disease progression biomarkers. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A24.
Abstract A36: Resveratrol decreases DNMTs in a human relevant animal model of breast cancer
Authors: Qin Wenyi , Zhu Weizhu , Zhang Ke , Clarke Kaitlin , Sauter Edward R. 
Published: January 2014

Abstract
Background: We previously reported that trans-resveratrol (resveratrol) downregulates the expression DNA methyltransferases (DNMTs) 1 and 3b in vitro and demethylates the tumor suppressor gene RASSF-1a in the breasts of women who are at increased breast cancer risk. Effects increased with increasing dose/circulating level of the agent, respectively. In the current report, we evaluated the effect of increasing doses of resveratrol on mammary tumor formation in the ACI rat, which develops estrogen dependent mammary carcinoma.
Methods: 91 female rats were treated with a slow release estradiol (E2) pellet plus one of the following: 5 mg/kg/day (low dose) resveratrol (n=23), 25 mg/kg/day (high dose) resveratrol (n=22), 0.1mg/kg/week 5-azacytadine (aza) (n=22), an inhibitor of DNMTs which is used clinically, or control (n=24). The animals were examined weekly to assess for the presence of tumor formation. After 5.5 months of treatment, the animals were sacrificed and the mammary glands harvested, with half of each gland snap frozen and the other half formalin fixed. Specimens were evaluated by a board certified pathologist to confirm histology as benign or malignant. DNMT1 and 3b were measured by qRT-PCR.
Results: Tumor formation was lowest (18% of rats) in the high dose resveratrol and 5-aza groups, intermediate in the low dose resveratrol group (26%) and highest in control (33%). DMNT1 expression was significantly (p&lt;0.0001) influenced by treatment, while DNMT3b was not. On the other hand, DNMT3b expression was significantly (p&lt;0.0001) influenced by histology (benign vs. malignant), whereas DNMT1 was not.
Conclusion: Treatment with resveratrol and 5-aza significantly influence DNMT expression, which may be important in the prevention/delay of mammary tumor formation. This mechanism of action of resveratrol warrants further investigation.
Citation Format: Wenyi Qin, Weizhu Zhu, Ke Zhang, Kaitlin Clarke, Edward R. Sauter. Resveratrol decreases DNMTs in a human relevant animal model of breast cancer. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A36.
Abstract A48: Mevalonate pathway inhibitors as chemopreventive agents on lung cancer cell lines: p53 might be a potent regulator
Authors: Lee Hwa Young , Kim In Kyoung , Lee Hye In , Kang Hye Sun , Park Chan Kwon , Ha Jick Hwan , Kim Seung Joon , Lee Sang Haak 
Published: January 2014

Abstract
Objectives: The mevalonate pathway has a crucial role in maintaining cellular homeostasis by cholesterol synthesis and protein prenylation. The mevalonate pathway inhibitors, statins and bisphosphonates have long been tested as anticancer agents, but because of the tumor-selective mechanisms, effects of the drugs on lung cancer cells are not clear. P53, a central regulator of stress responses, can induce cell cycle arrest, cell death or senescence depending on the level of cellular stresses. The current study investigated the effects of simvastatin and zolendronic acid on non-small-cell lung cancer (NSCLC) cells and whether the anti-tumor effects are related to p53 mutation status.
Material and Methods: We treated 3 NSCLC cells, A549 (p53 wild type), H522 (mutant) and H358 (null), with simvastatin and zolendronic acid to investigate cellular proliferation, cell cycle activity, intracellular reactive oxygen species (ROS) and expression of p53 target genes.
Results: In light microscopy, simvastatin (1 uM) caused morphologic changes in all 3 NSCLC cells. The cytotoxicity assay revealed that the synergistic impact with simvastatin (1 uM) and zolendronic acid (50 uM) existed on 72 hour cell survival, which was shown only in p53-wt cells. Expression of cyclin D1, which is important regulator of G1 to S phase progression, was decreased in p53-null and mutant cells but increased in p53-wt cells. Intracellular ROS levels after 24 hour treatment with blends of simvastatin and zolendronic acid was increased more than 2-fold in p53-wt cells, not in p53-null or mutant cells. Regulation of oxidative stress by p53 was shown with the increased expression of p53 target genes, cytochrome c oxidase 2 and TP53-induced glycolysis and apoptosis regulator quantified by real-time polymerase chain reaction.
Conclusions: Our data indicate that taken together, combination of simvastatin and zolendronic acid may have synergistic anti-proliferative effects in lung cancer cells which have functional p53, and those are mediated by increased cellular oxidative stress leading to apoptosis rather than cell cycle arrest. Targeting mevalonate pathway has the potential for lung cancer prevention, by inducing cellular metabolic stress responses mediated by p53.
Citation Format: Hwa Young Lee, In Kyoung Kim, Hye In Lee, Hye Sun Kang, Chan Kwon Park, Jick Hwan Ha, Seung Joon Kim, Sang Haak Lee. Mevalonate pathway inhibitors as chemopreventive agents on lung cancer cell lines: p53 might be a potent regulator. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A48.
Abstract A63: Combination aspirin and/or calcium chemoprevention with colonoscopy in colorectal cancer prevention: Cost-effectiveness analyses
Authors: Pence Barbara C. , Belasco Eric J. , Lyford Conrad P. 
Published: January 2014

Abstract
Background: Evidence from large-scale studies has shown that low dose aspirin and calcium are effective low risk strategies for primary prevention of colorectal cancer (CRC). We analyzed the cost-effectiveness of combination aspirin and calcium chemoprevention used with colonoscopy for primary prevention of CRC, using population-based compliance rates.
Methods: Cost-effectiveness of prevention strategies was compared using Markov chain Monte Carlo simulations of a hypothetical population with 100,000 persons starting at 50 years of age with a colonoscopy compliance rate of 50%. Once adenomas were detected, colonoscopy was repeated every 4 years until no adenomas recurred. Our data sources included state transition rates, CRC incidence, and treatment complication rates determined from existing literature. Age-adjusted US standard population mortality rates were used and costs were estimated from Medicare reimbursement data. The target population was US adults, ages 50 to 75, for whom primary CRC prevention methods are recommended within the general population.
Results: Outcome measures included incremental cost-effectiveness ratios, life-years saved, and cancer-free years saved. Compared to no screening, colonoscopy with aspirin and colonoscopy with both aspirin and calcium were the most cost-effective strategies based on cancer-free years saved. In terms of overall life-years saved, colonoscopy with and without aspirin were the most cost-effective options.
Conclusion: Given its strong positive effect, an aspirin-calcium regimen should be recommended for use in primary colorectal cancer prevention, especially when colonoscopy is also utilized.
Impact: Cost-effectiveness analysis is a valid tool for comparing multiple chemoprevention/screening strategies in a population.
Citation Format: Barbara C. Pence, Eric J. Belasco, Conrad P. Lyford. Combination aspirin and/or calcium chemoprevention with colonoscopy in colorectal cancer prevention: Cost-effectiveness analyses. [abstract]. In: Proceedings of the Eleventh Annual AACR International Conference on Frontiers in Cancer Prevention Research; 2012 Oct 16-19; Anaheim, CA. Philadelphia (PA): AACR; Cancer Prev Res 2012;5(11 Suppl):Abstract nr A63.