bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human FAM154A (SAXO1) is a microtubule-stabilizing protein specific to cilia and related structures
Authors: Dacheux Denis , Roger Benoit , Bosc Christophe , Landrein Nicolas , Roche Emmanuel , Chansel Lucie , Trian Thomas , Andrieux Annie , Papaxanthos-Roche Aline , Marthan Roger , Robinson Derrick R. , Bonhivers Mélanie 
Published: February 2015

ABSTRACT
Cilia and flagella are microtubule-based organelles present at the surface of most cells, ranging from protozoa to vertebrates, in which these structures are implicated in processes from morphogenesis to cell motility. In vertebrate neurons, microtubule-associated MAP6 proteins stabilize cold-resistant microtubules through their Mn and Mc modules, and play a role in synaptic plasticity. Although centrioles, cilia and flagella have cold-stable microtubules, MAP6 proteins have not been identified in these organelles, suggesting that additional proteins support this role in these structures. Here, we characterize human FAM154A (hereafter referred to as hSAXO1) as the first human member of a widely conserved family of MAP6-related proteins specific to centrioles and cilium microtubules. Our data demonstrate that hSAXO1 binds specifically to centriole and cilium microtubules. We identify, in vivo and in vitro, hSAXO1 Mn modules as responsible for microtubule binding and stabilization as well as being necessary for ciliary localization. Finally, overexpression and knockdown studies show that hSAXO1 modulates axoneme length. Taken together, our findings suggest a fine regulation of hSAXO1 localization and important roles in cilium biogenesis and function.
The Rho-specific GAP protein DLC3 coordinates endocytic membrane trafficking
Authors: Braun Anja C. , Hendrick Janina , Eisler Stephan A. , Schmid Simone , Hausser Angelika , Olayioye Monilola A. 
Published: February 2015

ABSTRACT
Membrane trafficking is known to be coordinated by small GTPases, but the identity of their regulators, the guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs) that ensure balanced GTPase activation at different subcellular sites is largely elusive. Here, we show in living cells that deleted in liver cancer 3 (DLC3, also known as STARD8) is a functional Rho-specific GAP protein, the loss of which enhances perinuclear RhoA activity. DLC3 is recruited to Rab8-positive membrane tubules and is required for the integrity of the Rab8 and Golgi compartments. Depletion of DLC3 impairs the transport of internalized transferrin to the endocytic recycling compartment (ERC), which is restored by the simultaneous downregulation of RhoA and RhoB. We further demonstrate that DLC3 loss interferes with epidermal growth factor receptor (EGFR) degradation associated with prolonged receptor signaling. Taken together, these findings identify DLC3 as a novel component of the endocytic trafficking machinery, wherein it maintains organelle integrity and regulates membrane transport through the control of Rho activity.
Honeybees (Apis mellifera) exhibit flexible visual search strategies for vertical targets presented at various heights
Authors: Morawetz Linde , Chittka Lars , Spaethe Johannes 
Published: February 2015

When honeybees are presented with a colour discrimination task, they tend to choose swiftly and accurately when objects are presented in the ventral part of their frontal visual field. In contrast, poor performance is observed when objects appear in the dorsal part. Here we investigate if this asymmetry is caused by fixed search patterns or if bees can increase their detection ability of objects in search scenarios when targets appear frequently or exclusively in the dorsal area of the visual field.We trained individual honeybees to choose an orange rewarded target among blue distractors. Target and distractors were presented in the ventral visual field, the dorsal field or both. Bees presented with targets in the ventral visual field consistently had the highest search efficiency, with rapid decisions, high accuracy and direct flight paths. In contrast, search performance for dorsally located targets was inaccurate and slow at the beginning of the experimental phase, but bees increased their search performance significantly after a few foraging bouts: they found the target faster, made fewer errors and flew in a straight line towards the target. However, bees needed thrice as long to improve the search for a dorsally located target when the target’s position changed randomly between the ventral and the dorsal visual field. We propose that honeybees form expectations of the location of the target’s appearance and adapt their search strategy accordingly. A variety of possible mechanisms underlying this behavioural adaptation, for example spatial attention, are discussed.
Microarray-based Simultaneous Quantitation of Six Serum Tumor Markers Improves Cancer Diagnostics
Authors: Ryabykh Tatiana P. , Osipova Tatiana V. , Sokolova Zinaida A. , Paklin Nikolay B. 
Published: February 2015

Relative low sensitivity and specificity of the known protein tumor markers limit their application in cancer diagnosis and screening. Simultaneous use of their combination can improve diagnostic efficacy. The aim of the study was to estimate diagnostic characteristics of the hydrogel microarray-based test system “TM-Biochip” developed in EIMB RAS for a simultaneous quantitation of six tumor markers: alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA), prostate-specific antigen (PSA total and free), neuron-specific enolase (NSE) and human chorionic gonadotropin (hCG) and to compare them with the diagnostic characteristics of systems used for estimating one tumor marker. Diagnostic performance of “TM-Biochip” system was evaluated using 170 serum samples from clinically confirmed cancer patients and control group patients. To evaluate the diagnostic efficiency of the test system we used linear regression and multiple logistic regression (LR), receiver operating characteristic (ROC) curve analysis and area under ROC curves (AUC). It was demonstrated that this new diagnostic system allows assessing the level of each marker and gives data comparable with the results obtained by measurement of each individual marker in a respective conventional ELISA system. ROC analysis and evaluation of diagnostic sensitivity and specificity of the system demonstrated that the new system did better than each of individual markers when classified: (1) cancer patients vs non-cancer patients; (2) patients with colorectal cancer (CRC) vs the other patients: patients with the other cancers and non-cancer patients; (3) patients with CRC vs patients with the other malignant tumors and (4) patients with prostate cancer (PC) vs patients with benign prostatic hyperplasia (BPH). In classification of the patients with PC and the patients with BPH AUC for the combination of six markers (LR6) was significantly higher than AUCs for each of individual markers, including the greatest AUC for PSAt (0.894 vs 0.771, p=0.032). On addition of such parameter as “age of patient” to the combination of six markers (LR7), AUC raised till 0.930 (p=0.01). Specificity at 90% sensitivity for PSAt, LR6 and LR7 accounted for 20 % (5.7-43.7), 60% (36.1-80.9) and 80.6 % (55.5-95.0), respectively. These results showed that “TM-Biochip” system is more efficient in revealing of cancer patients.
Structure and Properties of Substituted Gold Clusters
Authors: Yarzhemsky V.G. , Kazaryan M.A. , Bulychev N.A. , Muraviev E.N. , Dyakov Yu A. , Kosheleva O.K. , Chen C.H. 
Published: February 2015

Structure and stability of some substituted gold nanoparticles were investigated by quantum chemical calculations. It was found that terminal SH and SiO4H3 groups can substitute gold atoms without changes of regular structure in positions, where the number of Au-Au bonds does not exceed three. It was also revealed that neutral and charged endohedral clusters Au12M (M = Hf, Ta, W, Re, Os) form stable symmetrical or quasi-symmetrical structures for different values of total cluster charge. The latter property makes possible attachment/ detachment of functional ligands, and could have a potential for drug delivery.
Diagnosis of Type 1 and Type 2 Myocardial Infarction Using a High-Sensitivity Cardiac Troponin I Assay with Sex-Specific 99th Percentiles Based on the Third Universal Definition of Myocardial Infarction Classification System
Authors: Sandoval Yader , Smith Stephen W , Schulz Karen M , Murakami MaryAnn M , Love Sara A , Nicholson Jennifer , Apple Fred S 
Published: February 2015

Abstract

BACKGROUND
The frequency and characteristics of myocardial infarction (MI) subtypes per the Third Universal Definition of MI (TUDMI) classification system using high-sensitivity (hs) cardiac troponin assays with sex-specific cutoffs is not well known. We sought to describe the diagnostic characteristics of type 1 (T1MI) and type 2 (T2MI) MI using an hs–cardiac troponin I (hs-cTnI) assay with sex-specific cutoffs.

METHODS
A total of 310 consecutive patients with serial cTnI measurements obtained on clinical indication were studied with contemporary and hs-cTnI assays. Ninety-ninth percentile sex-specific upper reference limits (URLs) for the hs-cTnI assay were 16 ng/L for females and 34 ng/L for males. The TUDMI consensus recommendations were used to define and adjudicate MI based on each URL.

RESULTS
A total of 127 (41%) patients had at least 1 hs-cTnI exceeding the sex-specific 99th percentiles, whereas 183 (59%) had hs-cTnI within the reference interval. Females had more myocardial injury related to supply/demand ischemia than males (39% vs 18%, P = 0.01), whereas males had more multifactorial or indeterminate injury (52% vs 33%, P = 0.05). By hs-cTnI, there were 32 (10%) acute MIs, among which 10 (3%) were T1MI and 22 (7%) were T2MI. T2MI represented 69% (22 out of 32) of all acute MIs, whereas T1MI represented 31% (10 out of 32). Ninety-five patients (31%) had an increased hs-cTnI above the 99th percentile but did not meet criteria for acute MI. The most common triggers for T2MI were tachyarrhythmias, hypotension/shock, and hypertension. By contemporary cTnI, more MIs (14 T1MI and 29 T2MI) were diagnosed. By contemporary cTnI, there were 43 MIs, 14 T1MI, and 29 T2MI.

CONCLUSIONS
Fewer MI diagnoses were found with the hs-cTnI assay, contrary to the commonly accepted idea that hs-cTnI will lead to excessive false-positive diagnoses.
Narrating Sexual Compulsion: Gay Male Writing Beyond Shame
Authors: Alexander Jonathan 
Published: February 2015

Abstract
Throughout Edmund White’s long and distinguished career, the openly gay male author has not only created some of the richest representations of gay male identity and sociality, but he has also repeatedly reminded us that sex, pleasure, and, indeed, shame about sex and pleasure are at the heart of what it means to be queer. White writes almost compulsively about the nexus of shame and sexuality, promiscuity, and the compulsion to be sexual. But for White, sexual promiscuity shouldn’t necessarily be shameful; rather, the pursuit of sexual pleasures and the delight in identities based on sexuality are a potent part of what makes queer life what it is. A paradigmatic example of such potency is White’s powerful memoir, My Lives, which offers a startling, graphic, and challenging accounting of sexuality, shame, promiscuity, and pleasure—one often eclipsed in more domesticating rhetoric about queer lives. For White in particular, compulsion and compulsive narration become strategies to intervene in how we understand and figure normative gay male lives. To show the power—and necessity—of White’s work, this article contrasts it with recent gay fiction, particularly Tom Mendicino’s much lauded novel, Probation, to examine how sexual compulsion and promiscuity are figured for different aesthetic—and political—ends.
ePBLmed: application supporting classes in medical colleges conducted with the problem based learning method
Authors: Lipiak Tomasz , Piwowar Monika , Markiewicz Michał , Nowakowski Michał 
Published: February 2015

Abstract
This paper reviews the available e-learning applications used in teaching medicine and presents the functionalities of the Web application developed to support classes conducted with the problem-based learning (PBL) method. The application is a form of e-learning platform that allows posting as well as archiving content stored during PBL sessions; it is made up of three modules: the administration module, the teacher module, and the student module. The presented Web application is flexible in operation; it allows designing the number of groups and PBL sessions. The user, apart from using the system during PBL sessions, can access it at any place and time to supplement and update the content. The materials collected during individual PBL sessions constitute sets of content unique for each group because the learning objectives are different for them. With this approach, students can study considerably larger batches of material than it would be possible during classes conducted with other methods.
Modeling and optimization of maximum available work for irreversible
gas power cycles with temperature dependent specific heat
Authors: Açıkkalp Emin , Yamık Hasan 
Published: February 2015

Abstract
In classical thermodynamics,
the maximum power obtained from a system is
defined as exergy (availability). However, the term exergy is used for
reversible cycles only; in reality, reversible cycles do not exist, and all
systems are irreversible. Reversible cycles do not have such restrictions as
time and dimension, and are assumed to work in an equilibrium state. The
objective of this study is to obtain maximum available work for SI, CI and
Brayton cycles while considering the aforementioned restrictions and
assumptions. We assume that the specific heat of the
working fluid varies with temperature, we define optimum compression ratios and pressure ratio in order to obtain maximum available
work, and we discuss the results obtained. The design parameter most
appropriate for the results obtained is presented.
Crystal structure of a second polymorph of tetraphenylphosphonium perrhenate(VII), at 200 K, C24H20O4PRe
Authors: Mukiza Janvier , Gerber Thomas I. A. , Hosten Eric C. , Betz Richard 
Published: February 2015

Abstract C24H20O4PRe, tetragonal, I4̅ (no. 82), a = 12.2013(3) Å, c = 7.1016(2) Å, V = 1057.2 Å3, Z = 2, Rgt(F) = 0.0170, wRref(F2) = 0.0416, T = 200 K.
Structure and bonding of water molecules in zeolite hosts: Benchmarking plane-wave DFT against crystal structure data
Authors: Fischer Michael 
Published: February 2015

Abstract
Density-functional theory (DFT) calculations are widely employed to study the interaction of water molecules with zeolite frameworks. However, there have been only few attempts to assess whether these computations reproduce experimental structure data sufficiently well, especially with regard to the hydrogen positions of the water molecules. In this work, a detailed comparison between experimental crystal structures and DFT-optimised structures is made for six water-loaded natural zeolites. For each system, high-quality structure determinations from neutron diffraction data have been reported (bikitaite/Li–BIK, edingtonite/Ba–EDI, gismondine/Ca–GIS, scolecite/Ca–NAT, natrolite/Na–NAT, yugawaralite/Ca–YUG). Using a plane-wave DFT approach, the performance of six pure and three dispersion-corrected exchange-correlation functionals is compared, focusing on an optimisation of the atomic coordinates in a fixed unit cell (with cell parameters taken from experiment). It is found that the PBE and the PW91 functional give the smallest overall deviation between experiment and computation. Of the dispersion-corrected approaches, the PBE–TS functional exhibits the best performance. For the PBE and PBE–TS functionals, the agreement between experiment and DFT is analysed in more detail for different groups of interatomic distances. Regarding the OW–H distances in the water molecules, the DFT optimisations lead to physically realistic bond lengths. On the other hand, DFT has a systematic tendency to underestimate the length of hydrogen bonds. The cation-oxygen distances are mostly in very good agreement with experiment, although some exceptions indicate the necessity of further studies.
MOCVD of GaN-based HEMT structures on 8 inch silicon substrates
Authors: Laboutin Oleg , Lo Chien-Fong , Kao Chen-Kai , O’Connor Kevin , Johnson Wayne , Hill Daily 
Published: February 2015

ABSTRACTMetal organic chemical vapor deposition, as well as material and basic device properties of nitride-based high electron mobility transistor structures on (111) silicon substrates varying in diameter from 4 to 8 inch were studied using in-situ and ex-situ characterization techniques. All substrates used for the growth of the nitride structures in this study were of SEMI standard thicknesses. The total thickness of the nitride structures was in the range of 1.5 – 5 µm. It is reported that nitride structures can be grown on 4, 6 and 8 inch diameter substrates with very similar post-growth wafer shape, material and device characteristics. It is also shown that their crystal quality, 2DEG transport properties and isolation blocking voltages can be improved by increasing nitride structure thickness while maintaining post-growth wafer bow and warp less than 50 µm. The maximum thickness of nitride structures that can be successfully grown on 8 inch diameter SEMI standard substrates seems to be limited to about 4.5 µm due to plastic deformation of Si. Blocking voltages of more than 700 V were achieved using 4.5 µm thick nitride-based high electron mobility transistor structures grown on 8 inch Si substrate.
Evaluation of electron overflow in nitride-based LEDs influenced by polarization charges at electron blocking layers
Authors: Hayashi K. , Yasuda T. , Katsuno S. , Takeuchi T. , Kamiyama S. , Iwaya M. , Akasaki I. 
Published: February 2015

ABSTRACTWe have investigated an influence of positive polarization charges generated at an interface between GaN barrier/p-AlGaN EB (Electron Blocking) layer in a blue-LED. Simulation results suggested that such polarization charges caused an electron overflow from QWs. The simulation results also indicated that sufficient acceptor doping at the interface could neutralize the positive polarization charges and suppress the electron overflow. We then demonstrated the electron overflow caused by the positive polarization charges and its suppression with sufficient Mg doping at the interface by monitoring emissions from an additional second QW inserted between the p-EB layer and the p-GaN layer. Finally we conclude that the contribution of the electron overflow is not significant for the efficiency droop in blue-LEDs.
Fine-tuning B-site of a Chromite based Perovskite Catalyst for Steam Reforming of Glycerol
Authors: Umar Ahmed , Irvine John T.S. 
Published: February 2015

ABSTRACTThe perovskite catalysts La0.75Sr0.25Cr0.5X0.5O3±δ (where X= Mn, Fe and Co) were prepared by combustion synthesis and the influence of transition metals B-site substitution on the structural and catalytic properties of the catalysts in steam reforming of both pure and by-product glycerol to produce hydrogen-rich gases for utilization in solid oxide fuel cell was investigated. All the catalyst systems were found very active and selective with the Fe-substituted catalyst slightly more active. Hydrogen yield and coke suppression was better in the Mn-substituted catalyst which was also structurally more stable in fuel environment. Impregnation of Ni into the lattice structure of the pre-reacted both A-site and B-site stoichiometric catalysts from nickel nitrate solution and subsequent redox exsolution of Ni nano particles supported on the oxide surface of the materials has significantly improved the hydrogen yield by enhancing water-gas-shift reaction (WGSR). Thus, extent of the exsolution phenomenon observed in the materials followed the order LSCM > LSCC > LSCF.
GaN HEMT Drain-Lag Performance Dependence on GaN Channel Quality
Authors: Kamada Yoichi , Okamoto Naoya , Sato Masaru , Yamada Atsushi , Kotani Junji , Ishiguro Tetsuro , Ohki Toshihiro , Ozaki Shirou , Makiyama Kozo , Watanabe Keiji , Joshin Kazukiyo 
Published: February 2015

ABSTRACTIn this study, we investigated GaN channel layer quality to suppress drain-lag, which is an important parameter for switching performance. In this experiment, we confirmed that drain-lag performance has dependence on the tilt of the GaN channel layer. GaN channel layer with the tilt angle of 243 arcsec showed faster drain-lag recovery than the tilt angle of 209 arcsec. The results of the drain-lag test and isolation leakage current measurement indicated that the tilt angle and hopping distance contributed to drain-lag recovery. We proposed the mechanism of trap effect during the drain-lag test.
Impact of Knowledge and Information Technologies on Reading Comprehension/Impacto de las Tecnologías de la Información y el Conocimiento en la comprensión lectora
Authors: Trillos Pacheco Juan 
Published: February 2015

Este texto derivado de la investigación “La Lectura hipermedial y su relación con la comprensión lectora en estudiantes de Comunicación social”, analiza y estudia cómo con la aparición de la tecnología digital no solo se revolucionó el mundo dela imagen, el sonido y la forma de comunicarnos, sino cómo éstas han afectado el ámbito educativo al punto de plantear al hombre contemporáneo enormes retos para su debida y pedagógica implementación dentro de las aulas educativas. Debido a ello en el presente artículo se estudia si la lectura hipermedial modifica el grado de recordación y comprensión lectora en estudiantes de Comunicación social de la Universidad Autónoma del Caribe de Barranquilla.
An Approach to the Verbal Production Process: the Conceptual Side/Aproximación al proceso de producción verbal: la dimensión conceptual
Authors: Santiago Galvis Álvaro William 
Published: February 2015

Para efectos de analizar el proceso de producción verbal, en lo que a la dimensión conceptual respecta, en este artículo de revisión y reflexión, en primer lugar, se aborda la noción de lenguaje y se establece que éste es una facultad de la mente/cerebro del ser humano; enseguida se plantea que la lengua es una de las manifestaciones de dicha facultad, y como tal, un sistema de signos verbales cuya función básica es la comunicación. Con base en este principio se establece que la producción verbal es una actividad comunicativa que supone tres momentos: conceptualización, codificación verbal y realización fónica. Finalmente, se analiza el momento de conceptualización, centrando la atención en la noción de proposición.
The informative treatment of the Arab spring: Libya and its role on the Spanish front pages/El tratamiento informativo de la Primavera Árabe: Libia y su papel en las portadas españolas
Authors: Soria Ibañez María del Mar 
Published: February 2015

El conflicto internacional de Libia se convirtió en uno de los símbolos más cruentos de la lucha por la instauración, en el marco de la Primavera Árabe, de sistemas democráticos en países anclados en dictaduras unilaterales. El conflicto libio ocupó también numerosas portadas de periódicos en España y, por primera vez, se percibe que hay cierta unanimidad en el tratamiento informativo sobre una guerra que pronto se convirtió en conflicto internacional. El estudio revela el tratamiento informativo de este capítulo de transición política que tuvo un amplio eco internacional. Los principales resultados demuestran que el tratamiento mediático y su discurso estuvieron encaminados a la protección o defensa de la población civil y que la neutralidad en el enfoque fue la tónica general para reflejar los enfrentamientos entre insurgentes y fuerzas gadafistas.