bluuebunny/crossref_metadata_2025_split huggingface dataset:
A chromosome-level reference genome assembly and a full-length transcriptome assembly of the giant freshwater prawn (Macrobrachium rosenbergii)
Authors: Pootakham Wirulda , Sittikankaew Kanchana , Sonthirod Chutima , Naktang Chaiwat , Uengwetwanit Tanaporn , Kongkachana Wasitthee , Ampolsak Kongphop , Karoonuthaisiri Nitsara 
Published: July 2024

Abstract
The giant freshwater prawn (Macrobrachium rosenbergii) is a key species in the aquaculture industry in several Asian, African, and South American countries. Despite a considerable growth in its production worldwide, the genetic complexities of M. rosenbergii various morphotypes pose challenges in cultivation. This study reports the first chromosome-scale reference genome and a high-quality full-length transcriptome assembly for M. rosenbergii. We employed the PacBio High Fidelity (HiFi) sequencing to obtain an initial draft assembly and further scaffolded it with the chromatin contact mapping (Hi-C) technique to achieve a final assembly of 3.73-Gb with an N50 scaffold length of 33.6 Mb. Repetitive elements constituted nearly 60% of the genome assembly, with simple sequence repeats and retrotransposons being the most abundant. The availability of both the chromosome-scale assembly and the full-length transcriptome assembly enabled us to thoroughly probe alternative splicing events in M. rosenbergii. Among the 2,041 events investigated, exon skipping represented the most prevalent class, followed by intron retention. Interestingly, specific isoforms were observed across multiple tissues. Additionally, within a single tissue type, transcripts could undergo alternative splicing, yielding multiple isoforms. We believe that the availability of a chromosome-level reference genome for M. rosenbergii, along with its full-length transcriptome, will be instrumental in advancing our understanding of the giant freshwater prawn biology and enhancing its molecular breeding programs, paving the way for the development of M. rosenbergii with valuable traits in commercial aquaculture.
Optimal allocation method of shared energy storage in multienergy coupled microgrid with carbon emission constraint considering dual uncertainty of source and load
Authors: Li Manshu , Cui Xiaobo , Ma Lei , Tang Liang , Wang Gang , Rao Yao 
Published: July 2024

Abstract
In order to realize the stable operation of the multienergy coupled microgrid under the low-carbon constraint, a carbon emission constrained multienergy coupled microgrid shared energy storage optimization configuration method considering the dual uncertainty of source and load is proposed. This method constructs a source load double uncertainty and carbon emission model, and it analyzes the output characteristics and load demand characteristics of multiple coupled energy sources and the carbon emission results under these characteristics. According to the analysis results, the multienergy coupling microgrid shared energy storage optimization configuration model is constructed, and after the optimization configuration objective function and constraint conditions are determined, the bat algorithm is used to solve it, and the microgrid shared energy storage optimization configuration scheme that meets the objective function and constraint conditions is obtained. The test results show that after the method is applied, the utilization rate of multi-energy sources reaches 90.6%, and the expected carbon emissions are lower than 2.04 × 104kg. The microgrid fluctuation suppression rate reached 92.7%, and the net load fluctuation range was significantly reduced. Thus, it ensures reliable power supply of the microgrid and has good applicability.
Research on power battery recycling mode selection considering dual behavioral preferences under different government subsidies
Authors: Liu Juanjuan , Zhu Liangliang 
Published: July 2024

AbstractEfficient and stable recycling of power batteries requires the construction of a perfect and reasonable recycling system; the current research however fails to take into account the comprehensive influence of government policies outside the supply chain and internal members’ behavioral preferences at the same time, hence fail to reflect the whole and true recycling reality. This paper studies how different battery recycling subsidy policies and relevant players’ behavioral preferences affect decision-making along the supply chain and tries to work out the optimal power battery recycling model. The results show that (1) Which recycling subsidy policy is better at promoting battery recycling is related to the size of battery capacity; (2) Behavioral preferences of key players, i.e. the risk aversion of the battery manufacturer and the fairness concern preference of the vehicle manufacturer, can be irrelevant or have a negative impact on power battery recycling; (3) The preferred recycling models vary among key stakeholders in the recycling ecosystem. The government encourages battery producers to lead the recycling operation, consumers and battery producers check the risk sensitivity, and vehicle manufacturers prefer to recycle batteries alone.
The effects of β-mannanase supplementation on growth performance, digestive enzyme activity, cecal microbial communities, and short-chain fatty acid production in broiler chickens fed diets with different metabolizable energy levels
Authors: Zhang Lin , Huan Hailin , Zhang Kai , Tu Yuanlu , Yan Junshu , Zhang Hao , Xi Yumeng 
Published: July 2024

Abstract
This study assessed the effects of β-mannanase (BM) supplementation on growth performance, digestive enzyme activity, cecal microbial communities, and short-chain fatty acid (SCFA) production in broiler chickens fed diets with different metabolizable energy (ME) levels. A total of 1,296 male 1-d-old Cobb 500 broilers were randomly distributed in a 3 × 2 factorial arrangement (3 ME levels × 0 or 200 g/ton BM), with 6 replicates per treatment combination. The 3 ME levels were 3,000 (ME1), 2,930 (ME2), and 2,860 (ME3) kcal/kg, respectively, during the 0 to 3 wk-old stages and 3,150 (ME1), 3,080 (ME2), and 3,010 (ME3) kcal/kg, respectively, during the 3 to 6 wk-old stages. Reducing ME levels increased broiler feed intake (P = 0.036) and decreased average daily gain (ADG, P = 0.002) during the entire period. While BM supplementation increased ADG (P = 0.002) and improved the feed conversion ratio (P = 0.001) during the 0 to 3 wk-old stages, with no effect during the 3 to 6 wk-old stages. Overall, reducing ME levels increased pancreatic lipase (P = 0.045) and amylase (P = 0.013) activity and duodenal amylase activity (P = 0.047). Notably, BM supplementation significantly increased pancreatic lipase activity (P = 0.015) and increased lipase (P = 0.029) and amylase (P = 0.025) activities in the jejunal chyme. Although diet or enzyme supplementation did not affect microbial diversity, significant differences in microbial communities were observed. At the genus level, decreasing ME levels significantly affected the average abundances of Tyzzerella (P = 0.028), Candidatus_Bacilloplasma (P = 0.001), Vibrio (P = 0.005), and Anaerotruncus (P = 0.026) among groups, whereas BM supplementation reduced the average abundances of Escherichia-Shigella (P = 0.048) and increased the average abundances of Barnesiella (P = 0.047), Ruminococcus (P = 0.020), Alistipes (P = 0.050), and Lachnospiraceae_unclassified (P = 0.009). SCFA concentrations strongly depended on bacterial community composition, and BM supplementation increased acetic acid (P = 0.004), propionic acid (P = 0.016), and total SCFA concentrations. In conclusion, BM supplementation improved the performance of younger broilers, and both enzyme supplementation and reduced ME levels positively affected digestive enzyme activity and intestinal microflora.
Tracking procedural justice in stop and search pedestrian encounters with police body-camera records
Authors: Barber Mark , Tankebe Justice 
Published: July 2024

Abstract
Procedural justice has emerged as a crucial dimension of fairness in policing. It encompasses the extent to which police officers listen to citizens, show care for the well-being of citizens, act with neutrality, and show respect to them. These matters arise during police-citizen interactions, such as police stop and search of citizens. In this study, we draw on a random sample of 150 stop-and-search encounters recorded on police body-worn cameras in a police force in England (UK) to assess police adherence to procedural justice principles. The analysis shows high ratings on voice, neutrality, and respect but relatively low score on trustworthy motives. We found evidence of procedural justice variability across police jurisdictions. However, there was no evidence of an association between procedural justice and outcomes of stop-and-search interactions. The implications of the findings are discussed.
Move a little closer? Information sharing and the spatial clustering of bank branches
Authors: Qi Shusen , De Haas Ralph , Ongena Steven , Straetmans Stefan , Vadasz Tamas 
Published: July 2024

Abstract
We present a model of credit market competition to derive key hypotheses about how information sharing between banks influences the spatial clustering of their branches. We then test these hypotheses using data on 56,555 branches owned by 614 banks across 19 countries. We find that information sharing incentivizes banks to establish branches in localities that are new to them but that are already served by other banks. The resultant branch clustering is associated with reduced spatial credit rationing, as information sharing enables firms to access credit from more distant banks. These findings underscore how information sharing makes it more important for banks to move closer to each other rather than closer to their borrowers.
Dual-specificity phosphatases 13 and 27 as key switches in muscle stem cell transition from proliferation to differentiation
Authors: Hayashi Takuto , Sadaki Shunya , Tsuji Ryosuke , Okada Risa , Fuseya Sayaka , Kanai Maho , Nakamura Ayano , Okamura Yui , Muratani Masafumi , Wenchao Gu , Sugasawa Takehito , Mizuno Seiya , Warabi Eiji , Kudo Takashi , Takahashi Satoru , Fujita Ryo 
Published: July 2024

Abstract
Muscle regeneration depends on muscle stem cell (MuSC) activity. Myogenic regulatory factors, including myoblast determination protein 1 (MyoD), regulate the fate transition of MuSCs. However, the direct target of MYOD in the process is not completely clear. Using previously established MyoD knock-in (MyoD-KI) mice, we revealed that MyoD targets dual-specificity phosphatase (Dusp) 13 and Dusp27. In Dusp13:Dusp27 double knock-out mice, the ability for muscle regeneration after injury was reduced. Moreover, single-cell RNA sequencing of MyoD-high expressing MuSCs from MyoD-KI mice revealed that Dusp13 and Dusp27 are expressed only in specific populations within MyoD-high MuSCs, which also express Myogenin. Overexpressing Dusp13 in MuSCs causes premature muscle differentiation. Thus, we propose a model where DUSP13 and DUSP27 contribute to the fate transition of MuSCs from proliferation to differentiation during myogenesis.
Comparative Transcriptomic Analysis of Soybean Cyst Nematode Inbred Populations Non-adapted or Adapted on Soybean rhg1-a/Rhg4-Mediated Resistance
Authors: Kwon Khee Man , Masonbrink Rick E. , Maier Thomas R. , Gardner Michael N. , Severin Andrew J. , Baum Thomas J. , Mitchum Melissa G. 
Published: July 2024

Soybean cyst nematode (SCN, Heterodera glycines) is most effectively managed through planting resistant soybean cultivars, but the repeated use of the same resistance sources has led to a widespread emergence of virulent SCN populations that can overcome soybean resistance. Resistance to SCN HG type 0 (Race 3) in soybean cultivar Forrest is mediated by an epistatic interaction between the soybean resistance genes rhg1-a and Rhg4. We previously developed two SCN inbred populations by mass-selecting SCN HG type 0 (Race 3) on susceptible and resistant recombinant inbred lines, derived from a cross between Forrest and the SCN-susceptible cultivar Essex, which differ for Rhg4. To identify SCN genes potentially involved in overcoming rhg1-a/ Rhg4-mediated resistance, we conducted RNA sequencing on early parasitic juveniles of these two SCN inbred populations infecting their respective hosts, only to discover a handful of differentially expressed genes (DEGs). However, in a comparison with early parasitic juveniles of an avirulent SCN inbred population infecting a resistant host, we discovered 59 and 171 DEGs uniquely up- or downregulated in virulent parasitic juveniles adapted on the resistant host. Interestingly, the proteins coded by these 59 DEGs included vitamin B-associated proteins (reduced folate carrier, biotin synthase, and thiamine transporter) and nematode effectors known to play roles in plant defense suppression, suggesting that virulent SCN may exert a heightened transcriptional response to cope with enhanced plant defenses and an altered nutritional status of a resistant soybean host. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Neonatal Diazepam Exposure Decreases Dendritic Arborization and Spine Density of Cortical Pyramidal Neurons in Rats
Authors: Wadhwa Meetu , Sall Jeffrey W. , Chinn Gregory A. 
Published: July 2024

Objective:Benzodiazepines are extensively utilized in pediatric anesthesia and critical care for their anxiolytic and sedative properties. However, preclinical studies indicate that neonatal exposure to GABAergic drugs, including benzodiazepines, leads to long-term cognitive deficits, potentially mediated by altered GABAergic signaling during brain development. This preclinical study investigated the impact of early-life diazepam exposure on cortical neuronal morphology, specifically exploring dendritic arborization and spine density, crucial factors in synaptogenesis.Methods:Male and female Sprague Dawley rat pups were exposed to a single neonatal dose of diazepam (30 mg/kg) or vehicle on postnatal day (PND) 7. Golgi-Cox staining was used to assess cortical pyramidal neuron development at 4 developmental stages: neonatal (PND8), infantile (PND15), juvenile (PND30), and adolescence (PND42). Animals were randomized equally to 4 groups: male-vehicle, male-diazepam, female-vehicle, and female-diazepam. Neuronal morphology was evaluated after reconstruction in neurolucida, and dendritic spine density was analyzed through high-power photomicrographs using ImageJ.Results:Diazepam exposure resulted in decreased dendritic complexity in both sexes, with reduced arborization and spine density observed in cortical pyramidal neurons. Significant differences were found at each developmental stage, indicating a persistent impact. Dendritic length increased with age but was attenuated by diazepam exposure. Branching length analysis revealed decreased complexity after diazepam treatment. Spine density at PND42 was significantly reduced in both apical and basal dendrites after diazepam exposure.Conclusions:Neonatal diazepam exposure adversely affected cortical pyramidal neuron development, leading to persistent alterations in dendritic arborization and spine density. These structural changes suggest potential risks associated with early-life diazepam exposure. Further research is needed to unravel the functional consequences of these anatomic alterations.
Increased risk of subsequent primary lung cancer among female hormone-related cancer patients: A meta-analysis based on over four million cases
Authors: Wang Yan , Song Wenpeng , Wang Haoyu , Zhu Guonian , Li Yangqian , Wang Zhoufeng , Li Weimin , Che Guowei 
Published: July 2024

AbstractBackground:The incidence rate of lung cancer in women has significantly increased over the past decade, and previous evidence has indicated a significant relationship between the elevated levels of sex hormones and the risk of lung cancer. Therefore, we hypothesized that female hormone-related cancer (FHRC) patients, including breast, endometrial, cervical, and ovarian cancer patients, may experience a higher risk of developing subsequent lung cancer. This meta-analysis aimed to identify the risk of lung cancer among FHRC patients compared to the general population.Methods:The PubMed, Web of Science, EMBASE, Cochrane Library, and CNKI databases were searched up to May 11, 2022. Standardized incidence ratios (SIRs) with 95% confidence intervals (CIs) were used to identify the risk of subsequent lung cancer after FHRC. Subgroup analyses based on the follow-up time and tumor type were also conducted.Results:A total of 58 retrospective cohort studies involving 4,360,723 FHRC participants were included. The pooled results demonstrated that FHRC patients had a significantly increased risk of developing subsequent primary lung cancer (SIR = 1.61, 95% CI: 1.48–1.76,P<0.001). Subgroup analysis revealed an obvious trend of increasing lung cancer risk over time (SIRs for <5 years, ≥5 years, ≥10 years, ≥20 years, and ≥30 years after FHRC: 1.32, 1.59, 1.57, 1.68, and 1.95, respectively). In addition, subgroup analysis stratified by tumor type indicated an increased risk of developing subsequent lung cancer after breast (SIR = 1.25,P<0.001), endometrial (SIR = 1.40,P= 0.019), cervical (SIR = 2.56,P<0.001), and ovarian cancer (SIR = 1.50,P= 0.010).Conclusion:FHRC patients are more likely to develop lung cancer than the general population. Furthermore, the increased risk of subsequent primary lung cancer is more obvious with a longer survival time and is observed in all types of hormone-related cancer.Registration:International Platform of Registered Systematic Review and Meta-analysis Protocols: No. INPLASY202270044; https://inplasy.com/
Trends of diabetes in Beijing, China
Authors: Ma Aijuan , Lyu Jun , Dong Zhong , Nie Li , Xie Chen , Jiang Bo , Han Xueyu , Dong Jing , Zhao Yue , Li Liming 
Published: July 2024

Abstract

Background:
The global rise in diabetes prevalence is a pressing concern. Despite initiatives like “The Healthy Beijing Action 2020–2030” advocating for increased awareness, treatment, and control, the specific situation in Beijing remains unexplored. This study aimed to analyze the trends in diabetes prevalence, awareness, treatment, and control among Beijing adults.

Methods:
Through a stratified multistage probability cluster sampling method, a series of representative cross-sectional surveys were conducted in Beijing from 2005 to 2022, targeting adults aged 18–79 years. A face-to-face questionnaire, along with body measurements and laboratory tests, were administered to 111,943 participants. Data from all survey were age- and/or gender-standardized based on the 2020 Beijing census population. Annual percentage rate change (APC) or average annual percentage rate change (AAPC) was calculated to determine prevalence trends over time. Complex sampling logistic regression models were employed to explore the relationship between various characteristics and diabetes.

Results:
From 2005 to 2022, the total prevalence of diabetes among Beijing adults aged 18–79 years increased from 9.6% (95% CI: 8.8–10.4%) to 13.9% (95% CI: 13.1–14.7%), with an APC/AAPC of 2.1% (95% CI: 1.1–3.2%, P <0.05). Significant increases were observed among adults aged 18–39 years and rural residents. Undiagnosed diabetes rose from 3.5% (95% CI: 3.2–4.0%) to 7.2% (95% CI: 6.6–7.9%) with an APC/AAPC of 4.1% (95% CI: 0.5–7.3%, P <0.05). However, diabetes awareness and treatment rates showed annual declines of 1.4% (95% CI: −3.0% to −0.2%, P <0.05) and 1.3% (95% CI: −2.6% to −0.2%, P <0.05), respectively. The diabetes control rate decreased from 21.5% to 19.1%, although not statistically significant (APC/AAPC = −1.5%, 95% CI: −5.6% to 1.9%). Overweight and obesity were identified as risk factors for diabetes, with ORs of 1.65 (95% CI: 1.38–1.98) and 2.48 (95% CI: 2.07–2.99), respectively.

Conclusions:
The prevalence of diabetes in Beijing has significantly increased between 2005 and 2022, particularly among young adults and rural residents. Meanwhile, there has been a concerning decrease in diabetes awareness and treatment rates, while control rates have remained stagnant. Regular blood glucose testing, especially among adults aged 18–59 years, should be warranted. Furthermore, being male, elderly, overweight, or obese was associated with higher diabetes risk, suggesting the needs for targeted management strategies.
Tumor suppressive role of the antimicrobial lectin REG3A targeting the O-GlcNAc glycosylation pathway
Authors: Moniaux Nicolas , Geoffre Nicolas , Deshayes Alice , Dos Santos Alexandre , Job Sylvie , Lacoste Claire , Nguyen Tung-Son , Darnaud Marion , Friedel-Arboleas Mélanie , Guettier Catherine , Purhonen Janne , Kallijärvi Jukka , Amouyal Gilles , Amouyal Paul , Bréchot Christian , R. Vivès Romain , Buendia Marie Annick , Issad Tarik , Faivre Jamila 
Published: July 2024

Background and Aims:
Antimicrobial proteins of the regenerating family member 3 alpha (REG3A) family provide a first line of protection against infections and transformed cells. Their expression is inducible by inflammation, which makes their role in cancer biology less clear since an immune-inflammatory context may preexist or coexist with cancer, as occurs in HCC. The aim of this study is to clarify the role of REG3A in liver carcinogenesis and to determine whether its carbohydrate-binding functions are involved.

Approach and Results:
This study provides evidence for a suppressive role of REG3A in HCC by reducing O-GlcNAcylation in 2 mouse models of HCC, in vitro cell studies, and clinical samples. REG3A expression in hepatocytes significantly reduced global O-GlcNAcylation and O-GlcNAcylation of c-MYC in preneoplastic and tumor livers and markedly inhibited HCC development in REG3A-c-MYC double transgenic mice and mice exposed to diethylnitrosamine. REG3A modified O-GlcNAcylation without altering the expression or activity of O-linked N-acetylglucosaminyltransferase, O-linked N-acetylglucosaminyl hydrolase, or glutamine fructose-6-phosphate amidotransferase. Reduced O-GlcNAcylation was consistent with decreased levels of UDP-GlcNAc in precancerous and cancerous livers. This effect was linked to the ability of REG3A to bind glucose and glucose-6 phosphate, suggested by a REG3A mutant unable to bind glucose and glucose-6 phosphate and alter O-GlcNAcylation. Importantly, patients with cirrhosis with high hepatic REG3A expression had lower levels of O-GlcNAcylation and longer cancer-free survival than REG3A-negative cirrhotic livers.

Conclusions:
REG3A helps fight liver cancer by reducing O-GlcNAcylation. This study suggests a new paradigm for the regulation of O-GlcNAc signaling in cancer-related pathways through interactions with the carbohydrate-binding function of REG3A.