bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation and analysis of quinoa active protein (QAP) and its mechanism of inhibiting Candida albicans from a transcriptome perspective
Authors: Zhang Xufei , Zheng Chunmei , Ge Wenxuan , Li Xueying , Wang Xiuzhang , Sun Yanxia , Wu Xiaoyong 
Published: February 2025

The globe cultivation and industrial production of quinoa have been steadily increasing. Nevertheless, the full potential of quinoa’s nutritional and economic benefits remains underexploited. This study investigates the isolation and purification of quinoa active protein (QAP) through the precipitation method using ammonium sulfate from a phosphate extraction solution. The purification process involved gel filtration chromatography with Sephadex G-75 and Sephadex G-50 columns to obtain QAP fractions exhibiting inhibitory effects against Candida albicans (C. albicans). A comprehensive series of experiments was undertaken to examine the antifungal properties of these fractions. Proteomic analysis was employed to elucidate the composition of the active proteins. Furthermore, the activities of succinate dehydrogenase, Ca2+-Mg2+-ATPase, and catalase in C. albicans following treatment with QAP were quantified using an enzyme-linked immunosorbent assay. The effects of QAP on the cell morphology of C. albicans cultured on Spider agar medium was further investigated using scanning electron microscopy (SEM). Furthermore, RNA-seq analysis was conducted to investigate the alterations in gene expression in C. albicans cells subjected to QAP treatment. To elucidate the functional significance of these expression changes, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed. Quantitative real-time polymerase chain reaction was subsequently employed to validate the observed changes in gene expression. Our findings demonstrate that QAP exhibits inhibitory effects against C. albicans, with a minimum inhibitory concentration of 182 µg/mL. Through proteomic analysis, a definitive set of 18 active quinoa proteins was identified. At the molecular level, genes associated with hyphal development, cell wall and membrane integrity, cellular respiration, and energy metabolism were found to be enriched. Protein–protein interaction (PPI) analysis revealed that these QAP inhibit the growth of C. albicans hyphae, compromise cell wall and membrane integrity, and suppress oxidative phosphorylation. These disruptions collectively impair normal cellular metabolic activities, thereby exerting an inhibitory effect on C. albicans.
O Problema do Caixeiro Viajante de Minimização da Emissão de CO2: Estado da Arte no Contexto da Descarbonização da Mobilidade Urbana
Authors: Santana Alexsandra 
Published: February 2025

Apresenta as variantes do Problema do Caixeiro Viajante [TSP], com a função objetivo de minimização da emissão de CO2. O TSP é um problema clássico de otimização combinatória, classificado como Problema NP-Difícil. Esta pesquisa justifica-se por identificar as contribuições metodológicas e lacunas de pesquisa, que justifiquem o escopo paradigmático do Problema do Caixeiro Viajante Sustentável [TSPSM], um novo problema de pesquisa, com a perspectiva mitigadora e de resiliência autoregenerativa. Emergente no contexto da descarbonização da mobilidade urbana, e cenário de avanço de mudanças e catástrofes climáticas. O TSPSM considera o alcance de carbono zero. O objetivo é identificar como a função objetivo das novas variantes do TSP minimiza a emissão de CO2. A pesquisa delimita-se aos Programas de Pós-Graduação em Ciência da Computação brasileiros e publicações internacionais. A metodologia da revisão utilizou termos de busca controlados, junto às Bibliotecas de Teses e Dissertações e bases internacionais: Science Direct, Springer e IEEE XPlorer. Como resultado da pesquisa no contexto brasileiro o indicador é de escassez. Quanto à pesquisa internacional, há tendência de crescimento entre 2010 e 2024, apesar de intervalos de pesquisa entre os períodos de 2011; 2013; 2014 e 2022. Com a análise do corpus de 102 artigos, conclui-se uma síntese classificatória do TSP de minimização de CO2, abrangendo o problema do roteamento de veículos em múltiplos contextos urbanos. Apesar de indicadores chaves para um desempenho de sustentabilidade, tais como as principais características de decisão, a abrangência dessas modelagens não suporta os parâmetros estocásticos que configuram a nova mobilidade urbana em processo de descarbonização. Assim, o TSPSM perspectiva a otimização de fatores tecnológicos mitigadores da emissão de CO2, conforme suporta a integração e restrições tecnológicas deste novo cenário.
Muscle hypertrophy following acquired neurogenic injury: systematic review and analysis of existing literature
Authors: Strano Camilla MM , Bosco Luca , Laurini Christian , Sferruzza Giacomo , Butera Carla , Falzone Yuri M. , Sorrenti Benedetta , Ratti Adele , Tufano Laura , Leonardi Luca , Merlonghi Gloria , Morino Stefania , Gerevini Simonetta , Del Carro Ubaldo , Garibaldi Matteo , Filippi Massimo , Previtali Stefano C 
Published: June 2024

AbstractObjectivesNeurogenic muscle hypertrophy (NMH) is a rare condition characterized by focal muscle hypertrophy caused by chronic partial nervous injury. Given its infrequency, underlying mechanisms remain poorly understood. Inspired by two clinical cases, we conducted a systematic review to gain insights into the different aspects of NMH.MethodsWe systematically searched online databases up until May 30, 2023, for reports of muscle hypertrophy attributed to acquired neurogenic factors. We conducted an exploratory analysis to identify commonly associated features. We also report two representative clinical cases.ResultsOur search identified 63 reports, describing 93 NMH cases, to which we added our two cases. NMH predominantly affects patients with compressive radiculopathy (68.4%), negligible muscular weakness (93.3%), and a chronic increase in muscle bulk. A striking finding in most neurophysiological studies (60.0%) is profuse spontaneous discharges, often hindering the analysis of voluntary traces. Some patients exhibited features consistent with more significant muscle damage, including higher creatine phosphokinase levels, muscle pain, and inflammatory muscle infiltration. These patients are sometimes referred to in literature as “focal myositis.” Treatment encompassed corticosteroid, Botulinum Toxin A, decompressive surgery, antiepileptic medications, and nerve blocks, demonstrating varying degrees of efficacy. Botulinum Toxin A yielded the most favorable response in terms of reducing spontaneous discharges.InterpretationThis systematic review aims to provide a clear description and categorization of this uncommon presentation of an often‐overlooked neurological disorder. Though questions remain about the underlying mechanism, evidence suggests that aberrant fiber overstimulation along with increased workload that promotes focal damage may result in muscle hypertrophy. This may serve as a guide for therapeutic interventions.
Design and comparison of particle swarm optimization tuned Kalman filter based linear quadratic Gaussian controller and linear quadratic regulator for surface to air missile guidance system
Authors: Alitasb Girma Kassa , Beyene Getasew Mekonnen , Salau Ayodeji Olalekan 
Published: June 2024

AbstractThe study of missile guidance systems is a well‐known nonlinear control engineering area of research. To enhance the control performance of a missle guidance system, several technologies have been proposed in existing works. To resolve the weighting matrix selection issue of a linear quadratic Gaussian (LQG) controller for the surface‐to‐air missile guidance control system, this study utilizes the particle swarm optimization (PSO) technique. Selecting the best state (Q) and input (R) weighting matrices is a significant difficulty in the design of the LQG controller for real‐time applications since it affects the controller's performance and optimality. The weighting matrices are often chosen by a trial‐and‐error method that not only complicates the design but also does not yield optimal outcomes. Therefore, in this paper, a PSO method is developed and used in the design of the linear quadratic regulator (LQR) and LQG controllers for the surface‐to‐air missile control system to choose the elements of the Q and R matrices in the best possible way. Finally, a comparative analysis between the designed controllers was presented. The results shows that a good performance was achieved by using the proposed PSO‐tuned design process. The LQG and LQR are designed by manually adjusting the weighting matrices and utilizing an intelligent procedure, PSO algorithm which achieved optimal results. Further results indicate that the designed controllers, the PSO tuned LQR and LQG achieved a better performance over the manually adjusted LQR and LQG controllers.
A Revisit of Crystallization in Tin Halide Perovskite Thin Films: From Nucleation, Intermediate to Crystal Growth
Authors: Yin Mingyu , Yao Huanhuan , Qiu Hongju , Wu Cheng , Zhang Miao , Hao Feng 
Published: June 2024

AbstractPerovskite solar cells (PSCs) are exuding unique charm in the third generation of thin film solar cell technology with high power conversion efficiency (PCE) and low production cost. Recently, the lead‐free tin halide perovskite solar cells (TPSCs) have drawn significant research interests due to the advantages of low toxicity, near‐ideal bandgap and high carrier mobility. However, the rapid crystallization and easy oxidation limits the film quality and final performance of the solar cells. Therefore, the deposition of high‐quality tin halide perovskite films is key to the advance of TPSCs. In this review, the classical theory of solution nucleation and crystal growth is reviewed and correlated to the practical deposition of tin perovskite film. The physicochemical properties of tin perovskite with those of lead counterpart and the differences in the crystallization process is carefully compared. On this basis, recent regulation strategies are systematically summarized in terms of the nucleation management, intermediate phase engineering and crystal growth regulation. In addition, the preparation of mixed tin‐lead (Sn‐Pb) PSCs toward high performance is also discussed, as well as the limiting factors for device performance and stability. Finally, the future challenges and prospects of the crystallization regulation are discussed toward more efficient and stable TPSCs.
Innovations in Tactile Sensing: Microstructural Designs for Superior Flexible Sensor Performance
Authors: Wu Guancheng , Li Xiang , Bao Rongrong , Pan Caofeng 
Published: June 2024

AbstractTactile sensors have garnered considerable interest for their capacity to detect and quantify tactile information. The incorporation of microstructural designs into flexible tactile sensors has emerged as a potent strategy to augment their sensitivity to pressure variations, thereby enhancing their linearity, response spectrum, and mechanical robustness. This review underscores the imperative for progress in microstructured flexible tactile sensors. Subsequently, the discourse transitions to the prevalent materials employed in the fabrication of sensor electrodes, encapsulation layers, and active sensing mediums, elucidating their merits and limitations. In‐depth discussions are devoted to tactile sensors adorned with microstructures, including but not limited to, micropyramids, microhemispheres, micropillars, microporous configurations, microcracks, topological interconnections, multilevel constructs, random roughness, biomimetic microstructures inspired by flora and fauna, accompanied by exemplar studies from each category. Moreover, the utility of flexible tactile sensors within the realm of intelligent environments is explicated, highlighting their application in the monitoring of physiological signals, the detection of sliding motions, and the discernment of surface textures. The review culminates in a critical examination of the paramount challenges and predicaments that must be surmounted to further the development and enhance the functional performance of tactile sensors, paving the way for their integration into advanced sensory systems.
In2S3‐Gated Organic Photoelectrochemical Transistor with a Stable Transconductance
Authors: Zhou Bing‐Yu , Huang Yu‐Ting , Yuan Cheng , Lou Hao , Liu Xing‐Shi , Zhao Wei‐Wei , Chen Hong‐Yuan , Xu Jing‐Juan 
Published: June 2024

AbstractTransconductance (gm) is one important figure of merit for benchmarking organic electrochemical transistor (OECT) biosensors, which nevertheless experiences undesired fluctuation instead of keep constant during the target detection of varied concentrations. Light‐matter interplay is recently shown as a potential way to regulate the OECT characteristics, e.g. maximum gm at zero gate bias is achieved. In this work, the challenge of unstable gm is addressed by using a unique metal‐organic framework (MOF)‐derived hollow tube indium sulfide (m‐In2S3) as the photogate. Interestingly, the light irradiation on m‐In2S3 generate a straight transfer curve, leading to a stable gm that is desirable for biological application. Exemplifying by an aptasening, such a device is then tested for protein quantification with a stable sensitivity during the detection. This work features the feasibility of light‐matter interplay to achieve a stable gm in organic electronics.
3D Multispheroid Assembly Strategies towards Tissue Engineering and Disease Modeling
Authors: Zhu Tong , Hu Yan , Cui Haitao , Cui Haijun 
Published: June 2024

AbstractCell spheroids (esp. organoids) as 3D culture platforms are popular models for representing cell–cell and cell–extracellular matrix (ECM) interactions, bridging the gap between 2D cell cultures and natural tissues. 3D cell models with spatially organized multiple cell types are preferred for gaining comprehensive insights into tissue pathophysiology and constructing in vitro tissues and disease models because of the complexities of natural tissues. In recent years, an assembly strategy using cell spheroids (or organoids) as living building blocks has been developed to construct complex 3D tissue models with spatial organization. Here, a comprehensive overview of recent advances in multispheroid assembly studies is provided. The different mechanisms of the multispheroid assembly techniques, i.e., automated directed assembly, noncontact remote assembly, and programmed self‐assembly, are introduced. The processing steps, advantages, and technical limitations of the existing methodologies are summarized. Applications of the multispheroid assembly strategies in disease modeling, drug screening, tissue engineering, and organogenesis are reviewed. Finally, this review concludes by emphasizing persistent issues and future perspectives, encouraging researchers to adopt multispheroid assembly techniques for generating advanced 3D cell models that better resemble real tissues.
Biodegradable Cascade‐Amplified Nanotheranostics for Photoacoustic‐Guided Synergistic PTT/CDT/Starvation Antitumor in the NIR‐II Window
Authors: Kang Weiwei , Wang Yuhang , Xin Lei , Chen Lin , Zhao Keqi , Yu Lujie , Song Xiaorui , Zheng Ziliang , Dai Rong , Zhang Weiwei , Zhang Ruiping 
Published: June 2024

AbstractThe development of nanoassemblies, activated by the tumor microenvironment, capable of generating photothermal therapy (PTT) and amplifying the “ROS (·OH) storm,” is essential for precise and effective synergistic tumor treatment. Herein, an innovative cascade‐amplified nanotheranostics based on biodegradable Pd‐BSA‐GOx nanocomposite for NIR‐II photoacoustic imaging (PAI) guides self‐enhanced NIR‐II PTT/chemodynamic therapy (CDT)/starvation synergistic therapy. The Pd‐BSA‐GOx demonstrates the ability to selectively convert overexpressed H2O2 into strongly toxic ·OH by a Pd/Pd2+‐mediated Fenton‐like reaction at a lower pH level. Simultaneously, the GOx generates H2O2 and gluconic acid, effectively disrupting nutrient supply and instigating tumor starvation therapy. More importantly, the heightened levels of H2O2 and increased acidity greatly enhance the Fenton‐like reactivity, generating a significant “·OH storm,” thereby achieving Pd2+‐mediated cascade‐amplifying CDT. The specific PTT facilitated by undegraded Pd accelerates the Fenton‐like reaction, establishing a positive feedback process for self‐enhancing synergetic PTT/CDT/starvation therapy via the NIR‐II guided‐PAI. Therefore, the multifunctional nanotheranostics presents a simple and versatile strategy for the precision diagnosis and treatment of tumors.
Over 19% Efficient Inverted Organic Photovoltaics Featuring a Molecularly Doped Metal Oxide Electron‐Transporting Layer
Authors: Nugraha Mohamad Insan , Ling Zhaoheng , Aniés Filip , Ardhi Ryanda Enggar Anugrah , Gedda Murali , Naphade Dipti , Tsetseris Leonidas , Heeney Martin , Anthopoulos Thomas D. 
Published: June 2024

AbstractMolecular doping is commonly utilized to tune the charge transport properties of organic semiconductors. However, applying this technique to electrically dope inorganic materials like metal oxide semiconductors is challenging due to the limited availability of molecules with suitable energy levels and processing characteristics. Herein, n‐type doping of zinc oxide (ZnO) films is demonstrated using 1,3‐dimethylimidazolium‐2‐carboxylate (CO2‐DMI), a thermally activated organic n‐type dopant. Adding CO2‐DMI into the ZnO precursor solution and processing it atop a predeposited indium oxide (InOx) layer yield InOx/n‐ZnO heterojunctions with increased electron field‐effect mobility of 32.6 cm2 V−1 s−1 compared to 18.5 cm2 V−1 s−1 for the pristine InOx/ZnO bilayer. The improved electron transport originates from the ZnO's enhanced crystallinity, reduced hydroxyl concentrations, and fewer oxygen vacancy groups upon doping. Applying the optimally doped InOx/n‐ZnO heterojunctions as the electron‐transporting layers (ETLs) in organic photovoltaics (OPVs) yields cells with improved power conversion efficiency of 19.06%, up from 18.3% for devices with pristine ZnO, and 18.2% for devices featuring the undoped InOx/ZnO ETL. It is shown that the all‐around improved OPV performance originates from synergistic effects associated with CO2‐DMI doping of the thermally grown ZnO, highlighting its potential as an electronic dopant for ZnO and potentially other metal oxides.
Nanoarchitectonics in Advanced Membranes for Enhanced Osmotic Energy Harvesting
Authors: Wang Peifang , Tao Weixiang , Zhou Tianhong , Wang Jie , Zhao Chenrui , Zhou Gang , Yamauchi Yusuke 
Published: June 2024

AbstractOsmotic energy, often referred to as “blue energy”, is the energy generated from the mixing of solutions with different salt concentrations, offering a vast, renewable, and environmentally friendly energy resource. The efficacy of osmotic power production considerably relies on the performance of the transmembrane process, which depends on ionic conductivity and the capability to differentiate between positive and negative ions. Recent advancements have led to the development of membrane materials featuring precisely tailored ion transport nanochannels, enabling high‐efficiency osmotic energy harvesting. In this review, ion diffusion in confined nanochannels and the rational design and optimization of membrane architecture are explored. Furthermore, structural optimization of the membrane to mitigate transport resistance and the concentration polarization effect for enhancing osmotic energy harvesting is highlighted. Finally, an outlook on the challenges that lie ahead is provided, and the potential applications of osmotic energy conversion are outlined. This review offers a comprehensive viewpoint on the evolving prospects of osmotic energy conversion.
Sulfur‐Bridged Asymmetric CuNi Bimetallic Atom Sites for CO2 Reduction with High Efficiency
Authors: Sun Zhiyi , Li Chen , Wei Zihao , Zhang Fang , Deng Ziwei , Zhou Kejia , Wang Yong , Guo Jinhong , Yang Jiayi , Xiang Zequn , Ma Peijie , Zhai Huazhang , Li Shenghua , Chen Wenxing 
Published: June 2024

AbstractDouble‐atom catalysts (DACs) with asymmetric coordination are crucial for enhancing the benefits of electrochemical carbon dioxide reduction and advancing sustainable development, however, the rational design of DACs is still challenging. Herein, this work synthesizes atomically dispersed catalysts with novel sulfur‐bridged Cu‐S‐Ni sites (named Cu‐S‐Ni/SNC), utilizing biomass wool keratin as precursor. The plentiful disulfide bonds in wool keratin overcome the limitations of traditional gas‐phase S ligand etching process and enable the one‐step formation of S‐bridged sites. X‐ray absorption spectroscopy (XAS) confirms the existence of bimetallic sites with N2Cu‐S‐NiN2 moiety. In H‐cell, Cu‐S‐Ni/SNC shows high CO Faraday efficiency of 98.1% at −0.65 V versus RHE. Benefiting from the charge tuning effect between the metal site and bridged sulfur atoms, a large current density of 550 mA cm−2 can be achieved at −1.00 V in flow cell. Additionally, in situ XAS, attenuated total reflection surface‐enhanced infrared absorption spectroscopy (ATR‐SEIRAS), and density functional theory (DFT) calculations show Cu as the main adsorption site is dual‐regulated by Ni and S atoms, which enhances CO2 activation and accelerates the formation of *COOH intermediates. This kind of asymmetric bimetallic atom catalysts may open new pathways for precision preparation and performance regulation of atomic materials toward energy applications.
Decoration of Ag Species into Reduced Graphene Oxide Foam as a Superelastic and Robust Host toward Stable Zn Metal Anodes under Dwell‐Fatigue Condition
Authors: Zheng Ya Qi , Sun Peng Xiao , Zhang Xin Yu , Li Nian Wu , Wu Lili , Luan Deyan , Zhang Xitian , Lou Xiong Wen (David) , Yu Le 
Published: June 2024

AbstractDeep‐sea equipment usually operates under dwell‐fatigue condition, which means the equipped energy storage devices must survive under the changing pressure. Special mechanical designs should be considered to maintain the electrochemical performance of electrodes under this extreme condition. In this work, an effective assembly strategy is proposed to accommodate the dwell‐fatigue loading using Ag decorated reduced graphene oxide (rGO) foam (denoted as AGF) as a superelastic and robust Zn host. The wet‐press assembly process enables the formation of highly porous and robust framework. The strong synergetic effect between rGO and Ag further guarantees AGF's superelasticity and ultrahigh mechanical strength. Meanwhile, the homogeneously distributed Ag species on the rGO sheets act as zincophilic sites to effectively facilitate Zn plating. Furthermore, AGF offers enough space to address the expansion during the charge and discharge cycles. As expected, the symmetrical cell using this AGF@Zn host demonstrates a long lifespan over 400 h at a depth‐of‐discharge of 50%. It is worth mentioning that the superelastic AGF host realizes stable Zn plating/stripping under varying pressures.