bluuebunny/crossref_metadata_2025_split huggingface dataset:
Semidiurnal Tides on the Laptev Sea Shelf with Implications for Shear and Vertical Mixing
Authors: Janout Markus A. , Lenn Yueng-Djern 
Published: October 2013

Abstract
The Arctic continental shelf seas hold a globally significant source of freshwater that impacts Arctic Ocean stratification, circulation, and climate. This freshwater can be injected below the surface mixed layer by intense turbulent kinetic energy dissipation events, as resolved by Laptev Sea microstructure observations. The tides provide a major source of energy that can be dissipated and hence drive diapycnal mixing in the Laptev Sea. Multiyear ADCP mooring records from locations across the shelf reveal that semidiurnal tides are dominated by the M2 and S2 constituents, with the largest amplitudes on the outer shelf. Throughout most of the shelf, tides are clockwise polarized and sheared by stratification, as characteristic near the M2 critical latitude. Interannual variations of the tidal and shear structures on the inner shelf are mainly determined by the stratification-setting Lena River freshwater plume. In all locations, M2 tides are enhanced under sea ice, and therefore changes in the seasonal ice cover may lead to changes in tides and water column structure. The main conclusions of this study are that (i) tides play a comparatively greater role year-round on the outer shelf relative to the inner shelf; (ii) a sea ice reduction will overall decrease the predictability of the currents, especially on the inner shelf; and (iii) the freshwater distribution directly impacts diapycnal mixing by setting the vertical tidal structure. These combined effects imply that future sea ice loss will increase the variability and vertical mixing of freshwater, particularly on the inner shelf, where the Lena River first enters the Laptev Sea.
Airway hyperresponsiveness is associated with airway remodeling but not inflammation in aging Cav1
-/-
mice
Authors: Gabehart Kelsa E , Royce Simon G , Maselli Diego J , Miyasato Shelley K , Davis Elaine C , Tang Mimi LK , Jourdan Le Saux Claude 
Published: October 2013

Abstract

Background
Airway inflammation and airway remodeling are the key contributors to airway hyperresponsiveness (AHR), a characteristic feature of asthma. Both processes are regulated by Transforming Growth Factor (TGF)-β. Caveolin 1 (Cav1) is a membrane bound protein that binds to a variety of receptor and signaling proteins, including the TGF-β receptors. We hypothesized that caveolin-1 deficiency promotes structural alterations of the airways that develop with age will predispose to an increased response to allergen challenge.

Methods
AHR was measured in Cav1-deficient and wild-type (WT) mice 1 to 12 months of age to examine the role of Cav1 in AHR and the relative contribution of inflammation and airway remodeling. AHR was then measured in Cav1

-/-
and WT mice after an ovalbumin-allergen challenge performed at either 2 months of age, when remodeling in Cav1

-/-
and WT mice was equivalent, and at 6 months of age, when the Cav1

-/-
mice had established airway remodeling.

Results

Cav1

-/-
mice developed increased thickness of the subepithelial layer and a correspondingly increased AHR as they aged. In addition, allergen-challenged Cav1

-/-
mice had an increase in AHR greater than WT mice that was largely independent of inflammation. Cav1

-/-
mice challenged at 6 months of age have decreased AHR compared to those challenged at 2 months with correspondingly decreased BAL IL-4 and IL-5 levels, inflammatory cell counts and percentage of eosinophils. In addition, in response to OVA challenge, the number of goblet cells and α-SMA positive cells in the airways were reduced with age in response to OVA challenge in contrast to an increased collagen deposition further enhanced in absence of Cav1.

Conclusion
A lack of Cav1 contributed to the thickness of the subepithelial layer in mice as they aged resulting in an increase in AHR independent of inflammation, demonstrating the important contribution of airway structural changes to AHR. In addition, age in the Cav1

-/-
mice is a contributing factor to airway remodeling in the response to allergen challenge.
Improvement of care for the physical health of patients with severe mental illness: a qualitative study assessing the view of patients and families
Authors: van Hasselt Fenneke M , Oud Marian JT , Loonen Anton JM 
Published: October 2013

Abstract

Background
Patients with severe mental illness (SMI) experience more physical comorbidity than the general population. Multiple factors, including inadequate seeking of healthcare and health care related factors such as lack of collaboration, underlie this undesirable situation. To improve this situation, the logistics of physical health care for patients with SMI need to be changed. We asked both patients and their families about their views on the current organization of care, and how this care could be improved.

Methods
Group and individual interviews were conducted with patients and family of patients to explore their needs and preferences concerning the care for the physical health of patients with SMI, and to explore the shortcomings they had experienced. Using thematic analysis, responses were firstly divided into common topics, after which these topics were grouped into themes.

Results
Three major themes for the improvement of the physical care of patients with SMI were found. Firstly, the reduced ability of patients with SMI to survey their own physical health interests requires health care that is tailored to these needs. Secondly, the lack of collaboration amongst mental health care professionals and general practitioners (GPs) hinders optimal care. Thirdly, concerns were expressed regarding the implementation of monitoring and supporting a healthy lifestyle. Patients with SMI welcome this implementation, but the logistics of providing this care can be improved.

Conclusions
An optimal approach for caring for the physical health of patients with SMI requires a professional approach, which is different to the routine care provided to the general public. This approach can and should be accomplished within the usual organizational structure. However, this requires tailoring of the health care to the needs of patients with SMI, as well as structural collaboration between mental health care professionals and GPs.
Experimental research on intraocular aqueous flow by PIV method
Authors: Yang Hongyu , Song Hongfang , Mei Xi , Li Lin , Fu Xineng , Zhang Mindi , Liu Zhicheng 
Published: October 2013

Abstract

Background
Aqueous humor flows regularly from posterior chamber to anterior chamber, and this flow much involves intraocular pressure, the eye tissue nutrition and metabolism.

Purpose
To visualize and measure the intraocular flow regular pattern of aqueous humor.

Method
Intraocular flow in the vitro eyeball is driven to simulate the physiological aqueous humor flow, and the flow field is measured by Particle Image Velocimetry(PIV). Fluorescent particle solution of a certain concentration was infused into the root of Posterior Chamber(PC) of vitro rabbit eye to simulate the generation of aqueous and was drained out at a certain hydrostatic pressure from the angle of Anterior Chamber(AC) to represent the drainage of aqueous. PIV method was used to record and calculate the flow on the midsagittal plane of the eyeball.

Results
Velocity vector distribution in AC has been obtained, and the distribution shows symmetry feature to some extent. Fluorescent particle solution first fills the PC as it is continuously infused, then surges into AC through the pupil, flows upwards toward the central cornea, reflecting and scattering, and eventually converges along the inner cornea surface towards the outflow points at the periphery of the eyeball. Velocity values around the pupillary margin are within the range of 0.008-0.012 m/s, which are close to theoretical values of 0.0133 m/s, under the driving rate of 100 μl/min.

Conclusions
Flow field of aqueous humor can be measured by PIV method, which makes it possible to study the aqueous humor dynamics by experimental method. Our study provides a basis for experimental research on aqueous humor flow; further, it possibly helps to diagnose and treat eye diseases as shear force damage of ocular tissues and destructions on corneal endothelial cells from the point of intraocular flow field.
Ovarian HMW adiponectin is associated with folliculogenesis in women with polycystic ovary syndrome
Authors: Tao Tao , Xu Bing , Liu Wei 
Published: October 2013

Abstract

Background
Adiponectin may regulate ovarian steroidogenesis, folliculogenesis and ovulation. The alterations in the distribution of adiponectin multimers in follicular fluid (FF) and the relationship between adiponectin multimers and folliculogenesis in women with polycystic ovary syndrome (PCOS) remain unclear. In this study, we aimed to investigate the association between the levels of total and high molecular weight (HMW) adiponectin in serum and FF and folliculogenesis in women undergoing in vitro fertilisation (IVF).

Methods
This prospective study included ten Chinese women with PCOS and ten controls undergoing IVF. The levels of the total and HMW adiponectin in serum and FF were determined by ELISA. Insulin resistance (IR) was estimated using the homeostasis model assessment insulin resistance index (HOMA-IR).

Results
After controlling for the body mass index (BMI), the levels of the total, and the HMW adiponectin in the serum and FF were significantly lower in the women with PCOS compared with the normovulatory women undergoing IVF (P < 0.05). The levels of the HMW adiponectin were significantly lower in the FF than in the serum (P < 0.01). No significant differences were found in the total adiponectin levels in the serum and in the FF (P > 0.05). Decreased HMW adiponectin in the FF was associated with an increased number of follicles and decreased follicular diameters in the normovulatory and PCOS women, and this association was independent of the overall adiposity. A strong positive linear correlation was observed between the number of the follicles and the IR estimated by HOMA-IR (r = 0.784, P < 0.0001). We found that the larger follicular diameters had a negative relationship with the IR estimated by HOMA-IR (r = −0.445, P < 0.05). A strong negative linear correlation was observed between HOMA-IR and the HMW adiponectin levels (r = −0.726, P < 0.001) and the total adiponectin levels (r = −0.759, P < 0.001) in the FF.

Conclusions
The levels of the total adiponectin and the HMW adiponectin in the FF and serum were decreased in the Chinese women with PCOS compared with the normovulatory women undergoing IVF, and the differences persisted after controlling for the BMI. Ovarian HMW adiponectin is negatively correlated to folliculogenesis.
HIF-1α activation results in actin cytoskeleton reorganization and modulation of Rac-1 signaling in endothelial cells
Authors: Weidemann Alexander , Breyer Johannes , Rehm Margot , Eckardt Kai-Uwe , Daniel Christoph , Cicha Iwona , Giehl Klaudia , Goppelt-Struebe Margarete 
Published: October 2013

Abstract

Background
Hypoxia is a major driving force in vascularization and vascular remodeling. Pharmacological inhibition of prolyl hydroxylases (PHDs) leads to an oxygen-independent and long-lasting activation of hypoxia-inducible factors (HIFs). Whereas effects of HIF-stabilization on transcriptional responses have been thoroughly investigated in endothelial cells, the molecular details of cytoskeletal changes elicited by PHD-inhibition remain largely unknown. To investigate this important aspect of PHD-inhibition, we used a spheroid-on-matrix cell culture model.

Results
Microvascular endothelial cells (glEND.2) were organized into spheroids. Migration of cells from the spheroids was quantified and analyzed by immunocytochemistry. The PHD inhibitor dimethyloxalyl glycine (DMOG) induced F-actin stress fiber formation in migrating cells, but only weakly affected microvascular endothelial cells firmly attached in a monolayer. Compared to control spheroids, the residual spheroids were larger upon PHD inhibition and contained more cells with tight VE-cadherin positive cell-cell contacts. Morphological alterations were dependent on stabilization of HIF-1α and not HIF-2α as shown in cells with stable knockdown of HIF-α isoforms. DMOG-treated endothelial cells exhibited a reduction of immunoreactive Rac-1 at the migrating front, concomitant with a diminished Rac-1 activity, whereas total Rac-1 protein remained unchanged. Two chemically distinct Rac-1 inhibitors mimicked the effects of DMOG in terms of F-actin fiber formation and orientation, as well as stabilization of residual spheroids. Furthermore, phosphorylation of p21-activated kinase PAK downstream of Rac-1 was reduced by DMOG in a HIF-1α-dependent manner. Stabilization of cell-cell contacts associated with decreased Rac-1 activity was also confirmed in human umbilical vein endothelial cells.

Conclusions
Our data demonstrates that PHD inhibition induces HIF-1α-dependent cytoskeletal remodeling in endothelial cells, which is mediated essentially by a reduction in Rac-1 signaling.
Basal cell adenocarcinoma of the salivary gland: a morphological and immunohistochemical comparison with basal cell adenoma with and without capsular invasion
Authors: Jung Min Jung , Roh Jong-Lyel , Choi Seung-Ho , Nam Soon Yuhl , Kim Sang Yoon , Lee Sang-wook , Cho Kyung-Ja 
Published: October 2013

Abstract

Background
It is often difficult to diagnose salivary gland tumors that exhibit basaloid features differentially. The aim of this study was to identify additional morphological and immunohistochemical characteristics that can aid the diagnosis of basal cell adenocarcinoma (BCAC) of the salivary gland.

Methods and results
In total, 29 basal cell neoplasms [eight BCACs, 11 basal cell adenomas (BCAs) with capsular invasion, and 10 BCAs without capsular invasion] and 10 cases of adenoid cystic carcinomas (ACCs) were subjected to histopathology and immunohistochemical analyses for CK7, CK5/6, SMA, p63, calponin, p53, c-erbB2, CD117, β-catenin, EGFR, VEGF, Ki-67, and S100P protein expression. Compared to BCA without capsular invasion, the BCACs and BCAs with capsular invasion were more likely to be larger and have solid or cribriform patterns. Most BCACs and BCAs exhibited nuclear β-catenin expression. In all basal cell neoplasm cases, the clinical course after surgery with or without radiotherapy was indolent. β-catenin, CK5/6, CD117, and S100P protein were helpful for differentiating basal cell neoplasms from ACC.

Conclusions
BCAs with capsular invasion shared several pathological features with BCACs, including a large size and frequent cribriform patterns but the malignant potential of these tumors seems highly limited and should be reexamined. β-catenin immunostaining may aid the differential diagnosis between basal cell neoplasms and ACCs.

Virtual slides
The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/9637819101988153
Methodological comparisons for antimicrobial resistance surveillance in feedlot cattle
Authors: Benedict Katharine M , Gow Sheryl P , Checkley Sylvia , Booker Calvin W , McAllister Tim A , Morley Paul S 
Published: October 2013

AbstractBackgroundThe purpose of this study was to objectively compare methodological approaches that might be utilized in designing an antimicrobial resistance (AMR) surveillance program in beef feedlot cattle. Specifically, four separate comparisons were made to investigate their potential impact on estimates for prevalence of AMR. These included investigating potential differences between 2 different susceptibility testing methods (broth microdilution and disc diffusion), between 2 different target bacteria (non-type-specificE.coli[NTSEC] andMannheimia haemolytica), between 2 strategies for sampling feces (individual samples collected per rectum and pooled samples collected from the pen floor), and between 2 strategies for determining which cattle to sample (cattle that were culture-positive forMannheimia haemolyticaand those that were culture-negative).ResultsComparing two susceptibility testing methods demonstrated differences in the likelihood of detecting resistance between automated disk diffusion (BioMIC®) and broth microdilution (Sensititre®) for bothE.coliandM. haemolytica. Differences were also detected when comparing resistance between two bacterial organisms within the same cattle; there was a higher likelihood of detecting resistance inE.colithan inM. haemolytica. Differences in resistance prevalence were not detected when using individual animal or composite pen sampling strategies. No differences in resistance prevalences were detected inE.colirecovered from cattle that were culture-positive forM. haemolyticacompared to those that were culture-negative, suggesting that sampling strategies which targeted recovery ofE.colifromM. haemolytica-positive cattle would not provide biased results.ConclusionsWe found that for general purposes, the susceptibility test selected for AMR surveillance must be carefully chosen considering the purpose of the surveillance since the ability to detect resistance appears to vary between these tests depending upon the population where they are applied. Continued surveillance of AMR inM. haemolyticarecovered by nasopharyngeal swab is recommended if monitoring an animal health pathogen is an objective of the surveillance program as results of surveillance using fecalE.colicannot be extrapolated to this important respiratory pathogen. If surveillance ofE.coliwas pursued in the same population, study populations could target animals that were culture-positive forM. haemolyticawithout biasing estimates for AMR inE.coli. Composite pen-floor sampling or sampling of individuals per-rectum could possibly be used interchangeably for monitoring resistance inE.coli.
Cytochrome P450 genetic polymorphisms of Mexican indigenous populations
Authors: Sosa-Macías Martha , LLerena Adrián 
Published: October 2013

Abstract
This review focuses on the genetic polymorphisms of the cytochrome P450 (CYP) genes in Mexican indigenous populations, who are a part of the wide ethnic diversity of this country. These native groups have a particular historical trajectory that is different from the Mexican Mestizos. This variability may be reflected in the frequency distribution of polymorphisms in the CYP genes that encode enzymes involved in the metabolism of drugs and other xenobiotics. Therefore, these polymorphisms may affect drug efficacy and safety in indigenous populations in Mexico. The present study aimed to analyze the prevalence of CYP polymorphisms in indigenous Mexicans and to compare the results with studies in Mexican Mestizos. Because the extrapolation of pharmacogenetic data from Mestizos is not applicable to the majority of indigenous groups, pharmacogenetic studies directed at indigenous populations need to be developed. The Amerindians analyzed in this study showed a low phenotypic (CYP2D6) and genotypic (CYP2D6, CYP2C9) diversity, unlike Mexican Mestizos. The frequency of polymorphisms in the CYP1A1, CYP2C19, CYP2E1, and CYP3A4 genes was more similar among the Amerindians and Mexican Mestizos, with the exception of the CYP1A2 gene, whose *1F variant frequency in Mexican Amerindians was the highest described to date.
Estimating the Wishart Affine Stochastic Correlation Model using the empirical characteristic function
Authors: Da Fonseca José , Grasselli Martino , Ielpo Florian 
Published: October 2013

AbstractThis paper provides the first estimation strategy for the Wishart Affine Stochastic Correlation (WASC) model. We provide elements showing that the use of empirical characteristic function-based estimates is advisable as this function is exponential affine in the WASC case. We use a GMM estimation strategy with a continuum of moment conditions based on the characteristic function. We present the estimation results obtained using a dataset of equity indexes. The WASC model captures most of the known stylized facts associated with financial markets, including leverage and asymmetric correlation effects.
STAT3 Targets Suggest Mechanisms of Aggressive Tumorigenesis in Diffuse Large B-Cell Lymphoma
Authors: Hardee Jennifer , Ouyang Zhengqing , Zhang Yuping , Kundaje Anshul , Lacroute Philippe , Snyder Michael 
Published: October 2013

AbstractThe signal transducer and activator of transcription 3 (STAT3) is a transcription factor that, when dysregulated, becomes a powerful oncogene found in many human cancers, including diffuse large B-cell lymphoma. Diffuse large B-cell lymphoma is the most common form of non-Hodgkin’s lymphoma and has two major subtypes: germinal center B-cell−like and activated B–cell—like. Compared with the germinal center B-cell−like form, activated B-cell−like lymphomas respond much more poorly to current therapies and often exhibit overexpression or overactivation of STAT3. To investigate how STAT3 might contribute to this aggressive phenotype, we have integrated genome-wide studies of STAT3 DNA binding using chromatin immunoprecipitation-sequencing with whole-transcriptome profiling using RNA-sequencing. STAT3 binding sites are present near almost a third of all genes that differ in expression between the two subtypes, and examination of the affected genes identified previously undetected and clinically significant pathways downstream of STAT3 that drive oncogenesis. Novel treatments aimed at these pathways may increase the survivability of activated B-cell−like diffuse large B-cell lymphoma.
Saccharomyces cerevisiae Genes Involved in Survival of Heat Shock
Authors: Jarolim Stefanie , Ayer Anita , Pillay Bethany , Gee Allison C , Phrakaysone Alex , Perrone Gabriel G , Breitenbach Michael , Dawes Ian W 
Published: October 2013

Abstract
The heat-shock response in cells, involving increased transcription of a specific set of genes in response to a sudden increase in temperature, is a highly conserved biological response occurring in all organisms. Despite considerable attention to the processes activated during heat shock, less is known about the role of genes in survival of a sudden temperature increase. Saccharomyces cerevisiae genes involved in the maintenance of heat-shock resistance in exponential and stationary phase were identified by screening the homozygous diploid deletants in nonessential genes and the heterozygous diploid mutants in essential genes for survival after a sudden shift in temperature from 30 to 50°. More than a thousand genes were identified that led to altered sensitivity to heat shock, with little overlap between them and those previously identified to affect thermotolerance. There was also little overlap with genes that are activated or repressed during heat-shock, with only 5% of them regulated by the heat-shock transcription factor. The target of rapamycin and protein kinase A pathways, lipid metabolism, vacuolar H+-ATPase, vacuolar protein sorting, and mitochondrial genome maintenance/translation were critical to maintenance of resistance. Mutants affected in l-tryptophan metabolism were heat-shock resistant in both growth phases; those affected in cytoplasmic ribosome biogenesis and DNA double-strand break repair were resistant in stationary phase, and in mRNA catabolic processes in exponential phase. Mutations affecting mitochondrial genome maintenance were highly represented in sensitive mutants. The cell division transcription factor Swi6p and Hac1p involved in the unfolded protein response also play roles in maintenance of heat-shock resistance.