bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ascaris Mouse Model Protocols: Advancing Research on Larval Ascariasis Biology
Authors: de Almeida Lopes Camila , Wang Jianbin , Liffner Benjamin , Absalon Sabrina , Gazzinelli‐Guimaraes Pedro H. 
Published: June 2024

AbstractAscariasis, caused by both Ascaris lumbricoides and Ascaris suum, is the most prevalent parasitic disease worldwide, affecting both human and porcine populations. However, due to the difficulties of assessing the early events of infection in humans, most studies of human ascariasis have been restricted to the chronic intestinal phase. Therefore, the Ascaris mouse model has become a fundamental tool for investigating the immunobiology and pathogenesis of the early infection stage referred to as larval ascariasis because of the model's practicality and ability to replicate the natural processes involved. The Ascaris mouse model has been widely used to explore factors such as infection resistance/susceptibility, liver inflammation, lung immune‐mediated pathology, and co‐infections and, notably, as a pivotal element in preclinical vaccine trials. Exploring the immunobiology of larval ascariasis may offer new insights into disease development and provide a substantial understanding of key components that trigger a protective immune response. This article focuses on creating a comprehensive guide for conducting Ascaris experimental infections in the laboratory as a foundation for future research efforts. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Acquisition and embryonation of Ascaris suum eggs from adult femalesAlternate Protocol: Cleaning and purification of Ascaris suum from female A. suum uteriBasic Protocol 2: Preparation of Ascaris suum eggs and murine infectionBasic Protocol 3: Measurement of larval burden and Ascaris‐larva‐induced pathogenesisBasic Protocol 4: In vitro hatching and purification of Ascaris L3 larvaeSupport Protocol: Preparation of crude antigen from Ascaris infectious stagesBasic Protocol 5: Ultrastructure‐expansion microscopy (U‐ExM) of Ascaris suum larval stages
Lumbar Intrathecal Injection in Adult and Neonatal Mice
Authors: Stavrou Marina , Georgiou Elena , Kleopa Kleopas A. 
Published: June 2024

AbstractThis article describes a step‐by‐step process of lumbar intrathecal injection of Evans blue dye and AAV9‐EGFP in adult (2‐month‐old) and neonatal (postnatal day 10) mice. Intrathecal injection is a clinically translatable technique that has already been extensively applied in humans. In mice, intrathecal injection is considered a challenging procedure that requires a trained and experienced researcher. For both adult and neonatal mice, lumbar intrathecal injection is directed into the L5‐L6 intervertebral space. Intrathecally injected material enters the cerebrospinal fluid (CSF) within the intrathecal space from where it can directly access the central nervous system (CNS) parenchyma. Simultaneously, intrathecally injected material exits the CSF with pressure gradient and enters the endoneurial fluid and ultimately the peripheral nerves. While in the CSF, the injectable material also enters the bloodstream and systemic circulation through the arachnoid villi. A successful lumbar intrathecal injection results in adequate biodistribution of the injectable material in the CNS, PNS, and peripheral organs. When correctly applied, this technique is considered as minimally invasive and non‐disruptive and can be used for the lumbar delivery of any solute. © 2024 Wiley Periodicals LLC.Basic Protocol 1: C57BL/6 adult and P10 mice lumbar intrathecal injectionBasic Protocol 2: Tissue collection and preparation for evaluating Evans blue dye diffusionBasic Protocol 3: Tissue collection and preparation for immunohistochemistry stainingBasic Protocol 4: Tissue collection and vector genome copy number analysis
Managers, don't neglect your prospective employees to achieve your sustainability development goals
Authors: Sinha Richa , Saini Gordhan K. , Jawahar I. M. 
Published: June 2024

AbstractInstances of corporate social irresponsibility (CSI) in popular media, coupled with its negative consequences behooves a systematic assessment of the consequences of CSI. This study responds to calls for such research. Specifically, drawing on attribution theory, we investigate the role of CSI on prospective employees' employment intention (EI) and negative word‐of‐mouth (NWOM) propensity and the role of other condemning moral emotions (OCME) in mediating the relationship between CSI and EI and NWOM propensity. In addition, drawing on construal level theory, we investigate if psychological distance moderates the relationship between CSI and OCME, and thereby, impacts the mediation from CSI to EI and NWOM propensity through OCME. Results indicate that CSI decreases EI and enhances NWOM propensity, and these relationships are mediated via OCME. PD enhances the effect of CSI on EI and NWOM propensity through OCME such that lower PD is associated with a lower EI and higher NWOM propensity.
Converting CO2 Into Natural Gas Within the Autoclave: A Kinetic Study on Hydrogenation of Carbonates in Aqueous Solution
Authors: He Zhiwei , Zhang Hongbo 
Published: June 2024

AbstractCatalytic conversion of carbon dioxide (CO2) into value‐added chemicals is of pivotal importance, well the cost of capturing CO2 from dilute atmosphere is super challenge. One promising strategy is combining the adsorption and transformation at one step, such as applying alkali solution that could selectively reduce carbonate (CO32−) as consequences of CO2 adsorption. Due to complexity of this system, the mechanistic details on controlling the hydrogenation have not been investigated in depth. Herein, Ru/TiO2 catalyst was applied as a probe to elucidate the mechanism of CO32− activation, in which with thermodynamic and kinetic investigations, a compact Langmuir‐Hinshelwood reaction model was established which suggests that the overall rate of CO32− hydrogenation was controlled by a specific C−O bond rupture elementary step within HCOO− and the Ru surface was mainly covered by CO32− or HCOO− at independent conditions. This assumption was further supported by negligible kinetic isotope effects (kH/kD≈1), similarity on reaction barriers of CO32− and HCOO− hydrogenation (ΔH≠hydr,Na2CO3 and ΔH≠hydr,HCOONa) and a non‐variation of entropy (ΔS≠hydr≈0). More interestingly, the alkalinity of the solution is certainly like a two sides in a sword and could facilitate the adsorption of CO2 while hold back catalysis during CO32− hydrogenation.
Advancements in Manganese‐Based Cathode for Sustainable Energy Utilization
Authors: Zhou Ting , Wu Binjing , Li Chengze , Zhang Xinhuan , Li Wenting , Pang Huan 
Published: June 2024

AbstractManganese‐based compounds, especially manganese oxides, are one of the most exceptional electrode materials. Specifically, manganese oxides have gained significant interest owing to their unique crystal structures, high theoretical capacity, abundant natural availability and eco‐friendly nature. However, as transition metal semiconductors, manganese oxide possess low electrical conductivity, limited rate capacity, and suboptical cycle stability. Thus, combining manganese oxides with carbon or other metallic materials can significantly improve their electrochemical performance. These composites increase active sites and conductivity, thereby improving electrode reaction kinetics, cycle stability, and lifespan of supercapacitors (SCs) and batteries. This paper reviews the latest applications of Mn‐based cathodes in SCs and advanced batteries. Moreover, the energy storage mechanisms were also proposed. In this review, the development prospects and challenges for advanced energy storage applications of Mn‐based cathodes are summarized.
Eco‐Friendly Mechanochemical Synthesis of Bifunctional Metal Oxide Electrocatalysts for Zn‐Air Batteries
Authors: García‐Rodríguez M. , Cazorla‐Amorós D. , Morallón E. 
Published: June 2024

AbstractThe development of green and environmentally friendly synthesis methods of electrocatalysts is a crucial aspect in decarbonizing energy generation. In this study, eco‐friendly mechanochemical synthesis of perovskite metal oxide‐carbon black composites is proposed using different conditions and additives such as KOH. Furthermore, the optimization of ball milling conditions, including time and rotational speed, is studied. The mechanochemical synthesis in solid‐state conditions without additives produces electrocatalysts that exhibit the highest bifunctional electrochemical activity towards both oxygen reduction reaction (ORR) and oxygen evolution reaction (OER). Moreover, this synthesis demonstrates a lower Environmental Impact Factor (E‐factor), indicating its greener nature, and due to its simplicity, it has a great potential for scalability. The obtained bifunctional electrocatalysts have been tested in a rechargeable zinc‐air battery (ZAB) for 22 h with similar performance compared to the commercial catalyst (Pt/C) at significantly lower cost. These promising findings are attributed to the enhanced interaction between the perovskite metal oxide and carbon material and the improved dispersion of the perovskite metal oxide on the carbon materials.
Fine‐needle aspiration cytology of diffuse type tenosynovial giant cell tumor with malignant trasformation and review of literature
Authors: Shafi Saba , MacDonald William , Satturwar Swati 
Published: June 2024

AbstractTenosynovial giant cell tumors (TGCTs) arise from the synovium of joint, bursa, and tendon sheath. Diffuse type often affects large joints, has higher recurrence rates, metastases, and malignant transformation potential compared to the localized type. The cytopathology of TGCT, a fibrohistiocytic neoplasm distinct from other giant cell–rich soft tissue tumors, is rarely reported. Here we describe cytomorphology of a case of TGCT that was initially diagnosed on fine‐needle aspiration cytology (FNAC) consisting of a mixture of singly scattered polygonal or spindle mononuclear cells with hemosiderin laden macrophages, inflammatory cells, and a population of multinucleated osteoclast‐like giant cells. Persistent symptoms and repeat excision were consistent with high‐grade malignant transformation of the TGCT. Atypical cytologic features in a recurrent, infiltrative, or a metastatic lesion should raise suspicion for malignancy.
Neck Aging and Rejuvenation: A Balanced Approach to Assessment and Treatment
Authors: Roberts Wendy E. , Cheng Tsing 
Published: June 2024

ABSTRACTIntroductionThe neck is typically the earliest and most visible site of aging‐related esthetic changes; it is an area about which patients frequently express esthetic concerns and seek approaches to rejuvenation. This narrative review examines the current understanding of age‐related structural and cellular changes in the neck and skin, treatment approaches to neck rejuvenation, specifically noninvasive and minimally invasive approaches including skincare, and key patient factors to consider.MethodsA literature search of relevant publications was performed. Experience‐based recommendations for clinical assessment and treatment approach are provided to complement the current literature.ResultsNeck aging results from a combination of intrinsic factors, such as genetic background, and extrinsic factors, primarily ultraviolet light exposure. Positive treatment outcomes begin with thorough patient assessment, including neck aging status, patient‐specific intrinsic and extrinsic factors, and evaluation of skin type, which may help predict positive (e.g., skin tightening) and negative (e.g., dyspigmentation) dermal responses to specific procedures. In addition to skincare, a range of noninvasive, directed‐energy techniques (e.g., chromophore‐based lasers, ablative lasers, intense pulsed light, microfocused ultrasound, cryolipolysis, laser‐assisted lipolysis, and radiofrequency irradiation) and minimally invasive techniques (e.g., neurotoxins, microneedling, and fillers) are currently used for neck rejuvenation. A comprehensive approach to assessment and treatment contributes toward improved patient outcomes.ConclusionsThe neck aging process develops over time, and a prejuvenation strategy, including effective skincare, should be considered. The development of comprehensive noninvasive and minimally invasive techniques geared toward the neck and face as a single entity should provide better outcomes and fulfillment of patient esthetic goals for this critical area.
Stochastic dynamics of phycocyanin in years of contrasting phosphorus load
Authors: Carpenter Stephen R. , Brock William A. 
Published: June 2024

AbstractResilience, measured by the distribution of passage times between alternate states, indicates persistence of a state in stochastic dynamic systems such as blooms of cyanobacteria in lakes. We used high‐frequency datasets to compare the resilience of low and high states of phycocyanin, a pigment indicator of cyanobacteria, in Lake Mendota, Wisconsin, USA, for three growing seasons that ranged sevenfold in external phosphorus (P) load. Each year we observed 139–265 passage times across the unstable threshold that separated the low‐ from high‐phycocyanin states. Each sample of passage times is highly skewed with low median, larger mean, much larger SD, and wide tails extending to long lifetimes of a state. About 25% of events, whether low or high phycocyanin, lasted a day or more. Among these 3 years of contrasting external P load, there were no discernible differences in the resilience of either ecosystem state. We attribute this lack of contrast to the sustained recycling of P from sediments and the high stochasticity of phycocyanin in this lake.
Effect of Solvate Ionic Liquids in the Enantioselective (S)‐Proline‐Catalyzed Mechanochemical Robinson Annulation
Authors: Gamboa‐Velázquez Gonzalo , Arroyo‐Córdoba Ismael J. , Ávila‐Ortiz C. Gabriela , Naranjo‐Castañeda Carlos , Juaristi Eusebio 
Published: June 2024

AbstractHerein we report our observations from the study of the classical Robinson annulation reaction under solvent‐free mechanochemical activation by means of high‐speed ball milling. In particular, the enantioselective synthesis of the Wieland‐Miescher ketone was used as model reaction. (S)‐Proline was employed as chiral organocatalyst, either alone or in the presence of additives in order to determine their influence on the reaction's speed and stereoselectivity. Most interesting, among the additives that were examined, four solvate ionic liquids (SIL) afforded the desired product with significantly improved enantioselectivity relative to the mechanochemically‐activated reaction catalyzed by (S)‐proline alone. Furthermore, the required reaction time was reduced from 3 days to 90 min. The potential of other proline‐type organocatalysts was explored in this ball‐milling process. Initial attempts to increase the scope of the reaction to afford related annulation products were unsuccessful. HPLC‐MS experiments were carried out in order to detect some of the species that intervene in the mechanism of the (S)‐proline‐catalyzed Robinson cyclization reaction involving 2‐methyl‐1,3‐cyclohexanedione and methyl vinyl ketone.
Better integrating battery and fuel cells in electric vehicles
Authors: Boretti Alberto 
Published: June 2024

AbstractThis paper presents an innovative approach to enhancing the range of battery electric vehicles (BEVs) through the integration of a hydrogen fuel cell range extender. By combining the high energy efficiency of BEVs with the rapid refueling capability and extended range of hydrogen fuel cells, this hybrid system addresses the limitations of current electric vehicles in scenarios demanding longer driving ranges or quicker refueling options. Our study encompasses both experimental and theoretical analyses, leading to the proposal of a BEV configuration that includes a smaller battery complemented by a fuel cell range extender. The conventional fuel cell electric vehicle (FCV) examined relies exclusively on hydrogen fuel and features a minimal battery without plug‐in functionality, resulting in suboptimal energy economy. In contrast, our proposed BEV with a fuel cell range extender employs a larger battery capacity of 12 to 16 kWh alongside a downsized fuel cell stack and reduced hydrogen tank size. This configuration significantly improves energy recovery during braking and extends electric operation, thereby doubling the vehicle's energy economy. The proposed system not only enhances energy efficiency but also reduces the weight and volume of the overall energy storage system. Preliminary estimates suggest that the miles‐per‐gallon equivalent (MPGe) of this hybrid solution could exceed 140 over the US EPA certification cycle, outperforming existing PHEVs, BEVs, and FCVs.
Investigation of partial charging of enhanced ice storage systems
Authors: Laouer Abdelghani , Bellahcene Lahcene , Atia Aissa , Benhouia Amine Toufik , Teggar Mohamed 
Published: June 2024

AbstractPartial storage strategy can save energy and reduce emissions. In this study, analysis of the partial melting process of ice inserted with nanoparticles inside a square enclosure is investigated for thermal energy storage. The lattice Boltzmann method is for melting and heat transfer in the storage unit. The validation demonstrates strong concurrence between the current findings and the experimental data documented in the literature. The analysis is performed for various Rayleigh numbers, nanoparticle volume fractions, and their effect on melting time and energy storage. Two types of nanoparticles are tested that is, copper and alumina. The outcomes indicate that the Rayleigh number and volume fraction of nanoparticles have a significant impact on the phase change process. The nanoparticles addition leads to homogenous and hence expedited melting process including the final stage of the ice melting process which is very slow without nanoparticles. Furthermore, copper nanoparticles are slightly more effective than alumina. Moreover, using 6% copper nanoparticles can reduce the melting time by up to 12.4%.
Machine learning supported ground beef freshness monitoring based on near‐infrared and paper chromogenic array
Authors: Feng Yihang , Wang Yi , Beykal Burcu , Xiao Zhenlei , Luo Yangchao 
Published: June 2024

AbstractMaintaining freshness and quality is crucial in the meat industry, as lipid oxidation can lead to undesirable odors, flavors, and potential health risks. Traditional methods for assessing meat freshness often involve time‐consuming and destructive techniques, highlighting the need for rapid, noninvasive approaches. Recent advancements in spectroscopic and chromogenic sensor array technologies have opened up new avenues for monitoring meat quality parameters, offering the potential for real‐time, accurate, and cost‐effective solutions. As thiobarbituric acid reactive substances (TBARS) value is a classic indicator of meat lipid oxidation, this study investigated the data fusion of near‐infrared spectroscopy (NIR) and paper chromogenic array (PCA) for monitoring ground beef TBARS. A standardized PCA was fabricated by photolithography with nine chemoresponsive dyes. Changes in ground beef volatile organic compounds during storage were captured in the shifts of PCA color patterns. Nippy, an open‐source Python module, was used for automated NIR spectra preprocessing. The optimal preprocessing pipeline was found by 10‐fold cross‐validation in machine learning model development. Among optimized models, partial least square regression showed the best performance in coefficient of determination (R2) of .9477, root mean squared error of prediction of 0.0545 mg malondialdehyde/kg meat, and residual prediction deviation of 4.3717. The promising result of this study indicated the potential for NIR and PCA combinations to monitor TBARS values for ground beef freshness assessment.
Recent progress in covalent organic frameworks for flexible electronic devices
Authors: Wu Jinghang , Zhang Shiwei , Gu Qianfeng , Zhang Qichun 
Published: June 2024

AbstractCovalent organic frameworks (COFs) are porous materials with good crystallinity, highly ordered stacking, tunable channels, and diverse functional groups that have been demonstrated to show great potential applications in flexible electronic devices, including flexible energy storage devices (batteries and supercapacitors), memristors and sensors. Although great research progress on the usage of COFs as active elements in flexible electronics has been witnessed, the summary in this direction is rare. Thus, it is the right time to write a review on COFs‐based flexible electronics. In this review, we will first discuss the different synthesis strategies to prepare COF materials. Then, the applications of COFs in flexible electronic devices are summarized. Finally, the future performance improvement and development directions of COFs in the field of flexible electronic devices are briefly outlined. This review could provide basic concepts and some guidelines to stimulate novel applications of COFs in diverse flexible electronic devices.
Black American adolescent's efficacy in the face of discrimination
Authors: Glover Briah A. , Witherspoon Dawn P. 
Published: June 2024

AbstractThe pervasiveness of racism in the U.S. and its negative relations with key development outcomes has led researchers to uncover mediators, of which this article argues efficacy should be considered. Self‐efficacy, one's belief in their capability to accomplish a task or goal, can be measured in multiple domains of functioning and contexts to predict behaviour. The current study examines possible specificity in the relation between discrimination (general discrimination and school discrimination) and efficacy (academic efficacy and efficacy to combat discrimination), whether resilience moderates these associations, and if these processes differ by gender. A total of 879 Black youth (47% female; mean age = 12, SD = 0.58) were included in the analyses. Findings suggest that school discrimination experiences are differentially associated with domains of efficacy. Resilience was associated with increased efficacy, but no significant moderation effects were found.