bluuebunny/crossref_metadata_2025_split huggingface dataset:
Difficult catheter access to the occluded vessel during endovascular treatment of acute ischemic stroke is associated with worse clinical outcome
Authors: Ribo Marc , Flores Alan , Rubiera Marta , Pagola Jorge , Mendonca Nuno , Rodriguez-Luna David , Piñeiro Soco , Meler Pilar , Alvarez-Sabin Jose , Molina Carlos A 
Published: November 2012

Background and aimDuring endovascular procedures for acute ischemic stroke, catheter access to the occluded vessel may be technically difficult or impossible. The aim of this study was to access the impact of difficult catheter access to target the carotid artery on clinical outcome.MethodsAnterior circulation stroke patients undergoing transfemoral endovascular procedures where studied. Patients were divided into four groups according to time from groin puncture to target carotid catheterization quartiles. Patients in quartile 4 (Q4) were considered difficult carotid access. We defined several outcome measures: recanalisation (final Thrombolysis in Cerebral Infarction score ≥2a), time from groin puncture to recanalisation and favorable long term outcome (modified Rankin Scale score <3 at 3 months).ResultsOf 130 patients studied, carotid catheterization was impossible in seven patients (5.1%). These patients had significantly lower rates of recanalization (14.3% vs 80.5%; p<0.01) and favorable outcome (0% vs 36%; p=0.038). Among patients with an accessible carotid artery(n=123), median time from groin puncture to carotid catheterization was 20 min (IQR 10). A negative correlation between time to carotid access and recanalization was observed (r=−0.31; p<0.01). Patients in Q4 (>30 min) had lower rates of recanalization (60.7% vs 82.4%; p=0.02) and a lower favorable outcome (13.6% vs 41.3%; p=0.04). A logistic regression adjusted by age showed that baseline National Institutes of Health Stroke Scale score (OR 0.8; 95% CI: 0.72 to 0.92 p<0.01) and having difficult access (OR 1.3; 95% CI 1.3 to 20.1 p=0.018) independently predicted worse long term outcome.ConclusionsDifficult catheter access to target the carotid is common during acute endovascular treatment of stroke patients and is associated with a worse clinical outcome. If transfemoral access appears difficult, alternative access such as direct carotid puncture could be explored.
Chemical compaction of mudrocks in the presence of overpressure
Authors: Goulty N. R. , Ramdhan A. M. , Jones S. J. 
Published: November 2012

In sedimentary basins, compaction disequilibrium generates overpressure during rapid burial of fine-grained sediments in the mechanical compaction regime, at temperatures below ~70°C. Mudstones behave differently at greater depths in the chemical compaction regime, at temperatures above ~100°C, where evidence suggests that porosity reduction with increasing depth and temperature continues independently of effective stress up to high values of overpressure. We offer an explanation for this behaviour. The horizontal alignment of clay mineral grains is enhanced during clay diagenesis, creating sub-horizontal, flat pores. Because of their flexibility, the flat pores tend to close even under low values of normal effective stress acting across them. Thus, chemical compaction can proceed unless the net expulsion of pore water from the mudstones is inhibited sufficiently for the flat pores to be held open, which necessarily requires the pore pressure to approach the lithostatic stress.
In the Lower Kutai Basin, density log reversals are encountered in mudstones in the chemical compaction regime at depths of 3–4 km, where the pore pressure is close to the lithostatic stress. We attribute these reversals to the inhibition of dewatering during clay diagenesis at shallower depths, when the pore pressure was already close to lithostatic stress. Porosity was preserved by the very high pore pressure holding the flat pores open while the mudstone matrix was being cemented by the products of clay diagenesis. We coin the term ‘chemical undercompaction’ for this process.
Trapping of PARP1 and PARP2 by Clinical PARP Inhibitors
Authors: Murai Junko , Huang Shar-yin N. , Das Benu Brata , Renaud Amelie , Zhang Yiping , Doroshow James H. , Ji Jiuping , Takeda Shunichi , Pommier Yves 
Published: November 2012

Abstract
Small-molecule inhibitors of PARP are thought to mediate their antitumor effects as catalytic inhibitors that block repair of DNA single-strand breaks (SSB). However, the mechanism of action of PARP inhibitors with regard to their effects in cancer cells is not fully understood. In this study, we show that PARP inhibitors trap the PARP1 and PARP2 enzymes at damaged DNA. Trapped PARP–DNA complexes were more cytotoxic than unrepaired SSBs caused by PARP inactivation, arguing that PARP inhibitors act in part as poisons that trap PARP enzyme on DNA. Moreover, the potency in trapping PARP differed markedly among inhibitors with niraparib (MK-4827) &gt; olaparib (AZD-2281) &gt;&gt; veliparib (ABT-888), a pattern not correlated with the catalytic inhibitory properties for each drug. We also analyzed repair pathways for PARP–DNA complexes using 30 genetically altered avian DT40 cell lines with preestablished deletions in specific DNA repair genes. This analysis revealed that, in addition to homologous recombination, postreplication repair, the Fanconi anemia pathway, polymerase β, and FEN1 are critical for repairing trapped PARP–DNA complexes. In summary, our study provides a new mechanistic foundation for the rational application of PARP inhibitors in cancer therapy. Cancer Res; 72(21); 5588–99. ©2012 AACR.
Combined Administration of Rituximab and ON 013105 Induces Apoptosis in Mantle Cell Lymphoma Cells and Reduces Tumor Burden in a Mouse Model of Mantle Cell Lymphoma
Authors: Prasad Anil , Shrivastava Ashutosh , Papadopoulos Evangelos , Kuzontkoski Paula M. , Reddy M.V. Ramana , Gillum Amanda M. , Kumar Ramesh , Reddy E. Premkumar , Groopman Jerome E. 
Published: November 2012

Abstract
Purpose: Mantle cell lymphoma (MCL) is an incurable B-cell lymphoma, and new therapeutic strategies are urgently needed.
Experimental Design: The effects of ON 013105, a novel benzylstyryl sulfone kinase inhibitor, alone or with doxorubicin or rituximab, were examined in Granta 519 and Z138C cells. For in vivo studies, CB17/SCID mice were implanted subcutaneously with Z138C cells and treated with various combinations of ON 013105, doxorubicin, and rituximab. Tumor burden and body weight were monitored for 28 days.
Results: ON 013105 induced mitochondria-mediated apoptosis in MCL cells. Death was preceded by translocation of tBid to the mitochondria and cytochrome c release. In addition, ON 013105–treated cells exhibited reduced levels of cyclin D1, c-Myc, Mcl-1, and Bcl-xL. Using nuclear magnetic resonance (NMR) spectroscopy, we showed specific binding of ON 013105 to eIF4E, a critical factor for the initiation of protein translation. We proffer that this drug–protein interaction preferentially prevents the translation of the aforementioned proteins and may be the mechanism by which ON 013105 induces apoptosis in MCL cells. Efficacy studies in a mouse xenograft model showed that ON 013105 inhibited MCL tumor growth and that combining ON 013105 with rituximab reduced tumor burden further with negligible unwanted effects.
Conclusions: Our findings suggest that ON 013105, alone or in combination with rituximab, may be a potent therapeutic agent to treat MCLs. Clin Cancer Res; 19(1); 85–95. ©2012 AACR.
Social characteristics of psychological distress in disadvantaged areas of Berlin
Authors: Mundt Adrian , Kliewe T , Yayla S , Ignatyev Y , Busch MA , Heimann H , Heinz A , Rapp MA , Schouler-Ocak M , Ströhle A , Aichberger MC 
Published: November 2012

Purpose: Living in disadvantaged urban areas is associated with poor mental health. The purpose of this study was to assess which social characteristics were associated with psychological distress within a disadvantaged, multi-ethnic neighbourhood of Berlin. Methods: The study was conducted in an area of Berlin with the highest rates of unemployment and highest density of migrants. A total of 143 participants aged 18–57 years were included from a random sample. The social characteristics educational level, employment status, marital status, living alone, per-capita income and background of migration were collected. Psychological distress was assessed using the General Health Questionnaire GHQ-28; scores ≥ 5 indicated psychological distress corresponding to psychiatric caseness. Results: Psychological distress was found in 40.6% ( n = 58) of the sample. Psychological distress was associated with younger age (OR = 0.95, 95% CI = 0.92–0.98, p = .004), female gender (OR = 3.51, 95% CI = 1.55–7.92, p = .003) and living alone (OR = 3.88, 95% CI = 1.58–9.52, p = .003), but not with background of migration, low educational level or with unemployment. Conclusions: Young age and female gender may predispose for psychological distress in disadvantaged areas. Living alone could be a social indicator of poor mental health within disadvantaged urban areas. The directionality of the association is unclear. Background of migration, low income and educational level do not seem to be associated with poor mental health within those areas.
Effects of lichen extracts on haematological parameters of rats with experimental insulin-dependent diabetes mellitus
Authors: Çolak Suat , Geyikoğlu Fatime , Aslan Ali , Deniz Gülşah Yıldız 
Published: November 2012

The prevalence of diabetes mellitus in the world is steadily increasing. Oxidative stress contributes to the development of diabetic complications, including diabetic haematological changes. Lichens are used as food supplements and are also used as possible natural antioxidant, antimicrobial and anticancer agents. We hypothesized that antioxidant activity of lichens may decrease hyperglycaemia-induced oxidative stress and prevent the development of diabetic complications, including abnormality in haematological condition. Therefore, the effects of Cetraria islandica water extract (CIWE) and Pseudevernia furfuracea water extract (PFWE) on the haematological parameters of rats with type 1 DM were investigated for the first time in the present study. Control Sprague-Dawley or streptozotocin (STZ)-induced diabetic rats were either untreated or treated with water lichen extracts (5–500 mg/kg body weight (bw)/day) for 2 weeks, starting at 72 h after STZ injection. On day 14, animals were anaesthetized and haematological and metabolic parameters were determined between control and experimental groups. In addition, the total oxidative stress (TOS), a specific indicator of oxidative stress, and the total antioxidant capacity (TAC) were measured by biochemical studies. In diabetic rats, CIWE of 250–500 mg/kg bw dose showed more prominent results when compared with doses of PFWE for TAC. The results obtained in the present study suggested that the antioxidant activities of lichens might be the possible reason behind the observed antihaematological status. However, the protective effect of lichen extracts were inadequate on diabetes-induced microcytic hypochromic anaemia. In addition, the extracts have no effect on metabolic complications. Our experimental data showed that high doses of CIWE and PFWE alone have no detrimental effect on blood cells and TOS status of plasma. Hence, they are safe and suitable for different administration routes.
Relationship between carbon monoxide intoxication and sister chromatid exchange in lymphocytes
Authors: Tarik Ocak , Zeynep Ocak , Hasan Dogan , Mustafa Uzkeser , Ahmet Yesilyurt , Mevlit Ikbal , Şahin Aslan 
Published: November 2012

Aim: Carbon monoxide (CO) intoxication can be serious and is reported to be the cause of more than half of all fatal intoxications. In this study, we aimed to identify its genotoxic effects based on sister chromatid exchange (SCE). Materials and methods: CO-poisoned patients presented to the emergency services department were identified. Their demographic characteristics, vital findings, laboratory markers, source of CO gas, risk factors, and smoking habits were recorded. The genotoxic effect was assessed using the SCE method. Results: A total of 38 patients were recruited. Their ages ranged from 16–64 years (mean: 29.79 ± 10.92 years). In all the cases, the source of CO gas was a flash heater. The mean carboxyhemoglobin (COHb) level was 25.05 ± 7.15%. Of all the patients, 12 (31.6%) had a the Glasgow Coma Scale (GCS) scores of less than 15, and an important negative correlation was found between the GCS and COHb level ( r = −0.825; p < 0.001). Genotoxicity investigations revealed a significantly higher SCE frequency among patients with high COHb levels compared with that of control subjects with physiological COHb levels ( p < 0.001). However, no correlation between increased SCE frequency and COHb level was found ( r = 0.16; p = 0.34). Conclusion: CO poisoning was shown to result in genotoxicity via an increase in the frequency of SCE. This study is the first to demonstrate a genotoxic effect of CO independent of other chemicals.
Effects of Manidipine Plus Rosuvastatin Versus Olmesartan Plus Rosuvastatin on Markers of Insulin Resistance in Patients With Impaired Fasting Glucose, Hypertension, and Mixed Dyslipidemia
Authors: Liberopoulos Evangelos N. , Moutzouri Elisavet , Rizos Christos V. , Barkas Fotis , Liamis George , Elisaf Moses S. 
Published: November 2012

Aim: To compare the effect of manidipine 20 mg plus rosuvastatin 10 mg versus olmesartan 20 mg plus rosuvastatin 10 mg on markers of insulin resistance in patients with mixed dyslipidemia, hypertension, and impaired fasting glucose (IFG). Methods: This study had a prospective, randomized, open-label, blinded endpoint (PROBE) design. A total of 40 patients with IFG, mixed dyslipidemia, and stage 1 hypertension were included. Following dietary intervention, patients were randomly allocated to rosuvastatin (10 mg/d) plus olmesartan (20 mg/d) or manidipine (20 mg/d). The primary end point was the between-group difference in changes in the Homeostasis Model Assessment Insulin Resistance (HOMA-IR) index following 3 months of treatment. Secondary end points included changes in fasting plasma glucose (FPG), fasting insulin levels, and glucosylated hemoglobin. Results: At the end of the 3-month treatment period, a significant increase in HOMA-IR index by 14% (from 2.4 [0.5-7.9] to 2.7 [0.5-5.2], P = .02 versus baseline) was seen in the olmesartan plus rosuvastatin group. On the contrary, no significant change in HOMA-IR index was observed in the manidipine plus rosuvastatin group (1.7 [0.5-5.2] to 1.7 [0.8-6.0], P = NS versus baseline, P = .04 versus olmesartan plus rosuvastatin group). An increase in fasting insulin levels was observed in the olmesartan plus rosuvastatin group (+8%, from 10.1 [2.0-29.6] to 10.9 [2.0-19.1] μU/mL, P < .05 versus baseline), while no significant change was seen in the manidipine plus rosuvastatin group (+3%, from 7.3 [2.0-17.6] to 7.5 [1.9-15.6] μU/mL, P = NS versus baseline, P = .02 versus olmesartan plus rosuvastatin group). Fasting plasma glucose and glycosylated hemoglobin did not change significantly in any group. Conclusion: Manidipine seems to ameliorate the possible statin-associated increase in insulin resistance as compared with olmesartan in patients with IFG, hypertension, and mixed dyslipidemia.
Secondary genetic lesions in acute myeloid leukemia with inv(16) or t(16;16): a study of the German-Austrian AML Study Group (AMLSG)
Authors: Paschka Peter , Du Juan , Schlenk Richard F. , Gaidzik Verena I. , Bullinger Lars , Corbacioglu Andrea , Späth Daniela , Kayser Sabine , Schlegelberger Brigitte , Krauter Jürgen , Ganser Arnold , Köhne Claus-Henning , Held Gerhard , von Lilienfeld-Toal Marie , Kirchen Heinz , Rummel Mathias , Götze Katharina , Horst Heinz-August , Ringhoffer Mark , Lübbert Michael , Wattad Mohammed , Salih Helmut R. , Kündgen Andrea , Döhner Hartmut , Döhner Konstanze 
Published: November 2012

AbstractIn this study, we evaluated the impact of secondary genetic lesions in acute myeloid leukemia (AML) with inv(16)(p13.1q22) or t(16;16)(p13.1;q22); CBFB-MYH11. We studied 176 patients, all enrolled on prospective treatment trials, for secondary chromosomal aberrations and mutations in N-/KRAS, KIT, FLT3, and JAK2 (V617F) genes. Most frequent chromosomal aberrations were trisomy 22 (18%) and trisomy 8 (16%). Overall, 84% of patients harbored at least 1 gene mutation, with RAS being affected in 53% (45% NRAS; 13% KRAS) of the cases, followed by KIT (37%) and FLT3 (17%; FLT3-TKD [14%], FLT3-ITD [5%]). None of the secondary genetic lesions influenced achievement of complete remission. In multivariable analyses, KIT mutation (hazard ratio [HR] = 1.67; P = .04], log10(WBC) (HR = 1.33; P = .02), and trisomy 22 (HR = 0.54; P = .08) were relevant factors for relapse-free survival; for overall survival, FLT3 mutation (HR = 2.56; P = .006), trisomy 22 (HR = 0.45; P = .07), trisomy 8 (HR = 2.26; P = .02), age (difference of 10 years, HR = 1.46; P = .01), and therapy-related AML (HR = 2.13; P = .14) revealed as prognostic factors. The adverse effects of KIT and FLT3 mutations were mainly attributed to exon 8 and tyrosine kinase domain mutations, respectively. Our large study emphasizes the impact of both secondary chromosomal aberrations as well as gene mutations for outcome in AML with inv(16)/t (16;16).
Weak contractive inequalities and compatible mixed monotone random operators in ordered metric spaces
Authors: Hussain N , Latif A , Shafqat N 
Published: November 2012

Abstract
The aim of this work is to define the notion of compatible random operators in a partially ordered metric space and prove some coupled random coincidence theorems for a pair of compatible mixed monotone random operators satisfying

(
ϕ
,
φ
)

-weak contractive conditions. These results present random versions and extensions of recent results of Ćirić and Lakshmikantham (Stoch. Anal. Appl. 27:1246-1259, 2009), Choudhury and Kundu (Nonlinear Anal. 73:2524-2531, 2010), Alotaibi and Alsulami (Fixed Point Theory Appl. 2011:44, 2011) and many others.
The interpretation of disease phenotypes to identify TSE strains following murine bioassay: characterisation of classical scrapie
Authors: Beck Katy E , Vickery Christopher M , Lockey Richard , Holder Thomas , Thorne Leigh , Terry Linda A , Denyer Margaret , Webb Paul , Simmons Marion M , Spiropoulos John 
Published: November 2012

Abstract
Mouse bioassay can be readily employed for strain typing of naturally occurring transmissible spongiform encephalopathy cases. Classical scrapie strains have been characterised historically based on the established methodology of assessing incubation period of disease and the distribution of disease-specific vacuolation across the brain following strain stabilisation in a given mouse line. More recent research has shown that additional methods could be used to characterise strains and thereby expand the definition of strain “phenotype”. Here we present the phenotypic characteristics of classical scrapie strains isolated from 24 UK ovine field cases through the wild-type mouse bioassay. PrPSc immunohistochemistry (IHC), paraffin embedded tissue blots (PET-blot) and Western blotting approaches were used to determine the neuroanatomical distribution and molecular profile of PrPSc associated with each strain, in conjunction with traditional methodologies. Results revealed three strains isolated through each mouse line, including a previously unidentified strain. Moreover IHC and PET-blot methodologies were effective in characterising the strain-associated types and neuroanatomical locations of PrPSc. The use of Western blotting as a parameter to define classical scrapie strains was limited. These data provide a comprehensive description of classical scrapie strain phenotypes on isolation through the mouse bioassay that can provide a reference for further scrapie strain identification.
Toward understanding the genetic basis of adaptation to high-elevation life in poikilothermic species: A comparative transcriptomic analysis of two ranid frogs, Rana chensinensis and R. kukunoris
Authors: Yang Weizhao , Qi Yin , Bi Ke , Fu Jinzhong 
Published: November 2012

Abstract

Background
Understanding how organisms adapt to high-elevation environments at a genome scale provides novel insights into the process of adaptive evolution. Previous studies have mainly focused on endothermic organisms, while poikilothermic species may have evolved different mechanisms to cope with high-elevation environments. In this context, we sequenced transcriptomes of a pair of closely related anuran species, Rana chensinensis and R. kukunoris, which inhabit respective low- and high-elevation habitats. By comparing the two transcriptomes, we identified candidate genes that may be involved in high-elevation adaption in poikilothermic species.

Results
Over 66 million sequence reads from each transcriptome were generated. A total of 41,858 and 39,293 transcripts for each species were obtained by de novo assembly. By comparing the orthologous transcripts, we identified 125 protein-coding genes that have likely experienced strong positive selection (Ka/Ks>1). In addition, 335 genes that may bear a signature of positive selection (1≥Ka/Ks>0.5) were also recognized. By considering their functions, fourteen candidate genes were determined to be likely involved in high-elevation adaptation, including two CYP genes, USP-1, and several others.

Conclusions
We identified a set of candidate genes that may have promoted adaptation of R. kukunoris to its high-elevation environment. These include several genes that have previously been associated with oxygen binding, response to UV radiation, and repair of free radical injury. Detailed molecular, physiological, and phenotypic studies are required to better understand the roles of these genes in improving the performance of R. kukunoris in a high-elevation environment. We have evidence for both convergent and divergent evolution between endothermic and poikilothemic species, but additional research across a wide range of organisms will be necessary to comprehend the complexity of high-elevation adaptation.