bluuebunny/crossref_metadata_2025_split huggingface dataset:
SYSTEMIC EFFECTS OF TOPICAL APPLICATION OF BECLOMETHASONE 17,21‐DIPROPIONATE—COMPARATIVE STUDIES WITH BETAMETHASONE 17‐VALERATE OINTMENT AND FLUOCINONIDE CREAM—
Authors: Shimao Shuhei 
Published: April 2015

ABSTRACTThe systemic effects and clinical effects of topical application of 0.025% beclomethasone 17,21‐dipropionate ointment (BDO) and cream (BDC) by occlusive dressing technique (ODT) to adult patients with psoriasis were studied comparatively using a double blind test with 0.12% betamethasone 17‐valerate ointment (BVO) and 0.05% fluocinonide cream (FC) as control drugs. Further, the systemic effects of simple application (SA) of BDO were studied in infant patients with atopic dermatitis.The observation of adult patients with psoriasis revealed that the plasma cortisol value in BVO and FC groups decreased significantly as compared with that in BDO and BDC groups. This tendency was more remarkable when comparing the BDO 20 g group and the BVO 20 g group as well as the BDC 20 g group and the FC 20 g group. There was also a significant decrease in the eosinophil count in BVO and FC groups as compared with that in BDO and BDC goups.In infants with atopic dermatitis treated with BDO 5 g or 10 g, the plasma cortisol value remained unchanged, but the eosinophil count decreased remarkably in both groups.No noticeable changes in blood glucose or urinary electrolytes (Na, K and Cl) were observed in adult psoriasis patients treated with BDO, BDC, BVO or FC or in infant atopic dermatitis patients treated with BDO.No significant differences in clinical effects were found between either the BDO and BVO groups or the BDC and FC groups.
PSEUDOPYOGENIC GRANULOMA
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTPapular lesions of pseudopyogenic granuloma were examined before and during treatment with systemic corticosteroid using both light and electron microscopy. Before treatment, these lesions showed a proliferation of vessels lined with plump endothelial cells and heavy lymphohistiocytic infiltrates with numerous eosinophils in the dermis. During treatment, flattened papules showed a dilatation of vessels lined with flattened endothelial cells, extravasation of red cells, discontinuity of endothelial basal lamina, and, ultimately, discontinuity of the endothelial cells themselves. Eosinophils were rarely found. From these findings, it can be said that the morphological effects of corticosteroid on microvasculature have in part been elucidated.
DELAYED HYPERSENSITIVITY CROSS‐REACTIONS AMONG SPOROTHRIX SPECIES IN SPOROTRICHOTIC PATIENTS
Authors: Ishizaki Hiroshi , Nakamura Yoichi , Kurata Yukio , Iwatsu Tokio , Wheat Robert W. 
Published: April 2015

ABSTRACTCutaneous delayed hypersensitivity to antigens prepared from Sporothrix schenckii, S. schenckii var. luriei (atypical S. schenckii), S. curviconia, S. inflata and S. ghanensis was tested in eleven cutaneous sporotrichotic patients. Cross‐reactions were observed in various degrees. Five normal controls were not reactive.
A COMPARATIVE STUDY OF THE FUNCTIONS OF EPIDERMAL LANGERHANS CELLS IN HAIRLESS MICE AND NUDE MICE
Authors: Masutani Mamoru , Iwahara Kunio 
Published: April 2015

ABSTRACTAn experimental examination of contact allergic hypersensitivity in hairless mice and nude mice was carried out using 2,4‐dinitro‐1‐chlorobenzene. In normal nude mice without T‐cells, we were unable to detect apposition of mononuclear cells to Langerhans cells. In hairless mice, however, we could detect apposition of mononuclear cells to Langerhans cells after both sensitization and challenge procedures. Accordingly, hypotheses are proposed that mononuclear cells are T‐cells, and that Langerhans cells play the role of target cells which do not traverse the epidermis but remain within it.When we studied untreated hairless mice, a few immature Langerhans cells and indeterminate dendritic cells could be seen. Three hours after the challenge with 2,4‐dinitro‐1‐chlorobenzene, however, a great number of mature Langerhans cells were recognizable. The investigation of nude mice yielded reciprocal data.
EOSINOPHILIC PUSTULAR FOLLICULITIS
Authors: Saruta Takao , Nakamizo Yoshio 
Published: April 2015

ABSTRACT
Pustulosis pulmaris et plantaris‐like lesions occurring often concurrently were studied clinically and histologically with the following results.

The incidence of P.P.‐like lesions occurring concurrently with eosinophilic pustular folliculitis is high.
It increases and decreases almost in parallel with cutaneous lesions in other regions.
It can be differentiated clinically from pustulosis pulmaris et plantaris.
Cutaneous lesions are often seen on the elbow and knee in P.P. while it is often found in the face and back in eosinophilic pustular folliculitis.
Unlike P.P. and eczema, they have a histology specific to this disease.
They have a similarity to histology of the hair‐root sheath.

On the basis of the findings above, we think P.P. ‐like lesions are partial symptoms of this disease.
Since the palm and sole have no hair follicles, we think it is better to call this disease Eosinophilic pustular dermatosis.
ULTRASTRUCTURE OF SENILE KERATOSIS
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTThree cases of a hypertrophic type of senile keratosis were examined by an electron microscope. All three showed similar changes, although some variability from case to case was also observed. Lesional epidermis showed widened intercellular spaces with microvilli‐formation, a general increase in the bulk of the basal cells, a decrease in number of desmosomes, a decrease in the amount of tonofilaments, various degrees of aggregations of tonofibrils, binucleated keratinocytes, mitotic figures, prominent nucleoli, and an increase in number of nuclear bodies. In lesional dermoepidermal junctions, discontinuity of the basal lamina with cytoplasmic projections of the basal cells to the upper dermis was not infrequently observed. These changes are similar to those found in Bowen's disease, but are somewhat milder. In addition, in one case, keratinocytes of lesional epidermis showed lamellar structures closely associated with the rough endoplasmic reticulum. Significance of these structures is discussed.
CONTACT SENSITIVITY IN MICE INDUCED BY TOLYLENE DIISOCYANATE (TDI)
Authors: Tanaka Ken‐Ichi 
Published: April 2015

ABSTRACTContact sensitivity cannot be easily induced in mice by simple chemicals. The author has found that tolylene diisocyanate (TDI), which is widely used in industry, can produce a delayed type of hypersensitivity in mice. The delayed onset of the ear reaction and the histology of the ear swelling observed were characteristic of contact sensitivity. Adoptive transfer of contact sensitivity was possible in inbred mice. Since TDI is a causative agent of bronchial asthma, this discovery may be useful in clarifying the relationship between immediate and delayed types of hypersensitivity.
A CASE OF PLASMACYTOSIS WITH MULTIPLE PECULIAR ERUPTIONS
Authors: Kitamura Keijiro , Tamura Nobuya , Hatano Hitoshi , Toyama Keisuke , Mikata Atsuo , Watanabe Shaw 
Published: April 2015

ABSTRACTA 32‐year‐old man had been in good health until he noticed painful swelling of the lymph nodes in the left occipital region in spring of 1975. Half a year later, multiple, infiltrating erythematous or nodular lesions appeared on the anterior chest and then spread over almost his entire body except for the lower extremities. These skin lesions were reddish brown to purplish brown in color, and irregular in shape and size. All of his superficial lymph nodes were palpable, and pain was felt on pressure. No other systemic symptoms were noted.Laboratory findings showed polyclonal gammopathy; total protein, 9.6 g/dl; IgG, 4,900 mg/dl; IgA, 590 mg/dl and an increased erythrocyte sedimentation rate of 92 mm/hr. A skin biopsy revealed prominent perivascular proliferation of plasma cells and follicular hyperplasia in the dermis. Lymph node biopsy also revealed proliferation of plasma cells in both the paracortical area and medulla. No neoplastic growth was recognized.These pathological findings and polyclonal hypergammaglobulinemia were consistent with reactive plasmacytosis.The patient is now under observation, and the skin lesions and systemic lymphadenopathy have not been changed.This peculiar B‐cell proliferative disorder was discussed in relation to other diseases.
IMMUNOFLUORESCENCE STUDY OF PITYRIASIS LICHENOIDES CHRONICA
Authors: Hayashi Tsutomu , Kawada Akihiro 
Published: April 2015

ABSTRACTDeposits of IgM, C3 and fibrin, partly Clq, were found in the dermal vessel walls and along the dermal‐epidermal junction of skin lesions from patients with pityriasis lichenoides chronica. However, no circulating antibody to the blood vessel or the epidermal‐dermal junction was detected in both patients with pityriasis lichenoides chronica and pityriasis lichenoides et varioliformis acuta so far examined. The immunofluorescence pattern in pityriasis lichenoides chronica was identical to that observed in pityriasis lichenoides et varioliformis acuta. This supports the view that these two disorders are variants of a single disease process mediated by immune complexes.
SEROLOGICAL CROSS‐REACTIVITY AMONG KLEBSIELLA PNEUMONIAE K 47, CERATOCYSTIS, EUROPHIUM AND GRAPHIUM SPECIES
Authors: Ishizaki Hiroshi , Kurata Yukio , Nakamura Yoichi , Wheat Robert W. 
Published: April 2015

ABSTRACTCulture filtrate antigens of 52 of 56 Ceratocystis, 2 of 2 Europhium and 5 of 6 Graphium species were found to be serologically cross‐reactive with Klebsiella pneumoniae K 47 antiserum by use of double immunodiffusion precipitin and capillary precipitin tests.
MONGOLIAN SPOTS REMAINING IN SCHOOLCHILDREN
Authors: Kikuchi Ichiro 
Published: April 2015

ABSTRACT10,248 schoolchildren in central Okinawa were examined by one observer for remaining Mongolian spots. The spots were found in 783 schoolchildren (7.64%). Sacral Mongolian spots disappeared smoothly, while extrasacral Mongolian spots showed a general tendency toward disappearance. There were interesting but unexplainable differences in the rate of disappearance of Mongolian spots among city dwellers, suburban inhabitants and rural people in the area examined.
THREE‐DIMENSIONAL ARCHITECTURE OF EPITHELIAL SKIN TUMORS: AN APPLICATION OF EPIDERMAL SEPARATION
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTTrypsin separation of the epidermis from the dermis was applied to 14 benign, premalignant, and malignant epithelial skin tumors. The three‐dimensional architecture of the undersurfaces of these tumors was clearly observed in all cases.Seborrheic keratosis showed network‐like structures in young lesions and a reticulated type of lesion, but showed sharply demarcated mass‐like structures in well‐developed lesions and an acanthotic type of lesion.Bowen's disease, Paget's disease, and senile keratosis exhibited coarse network‐like structures similar to each other accompanied by a general decrease in the amount of melanin pigment. Boundaries between these tumors and the surrounding epidermis were more or less irregular and gradual.In superficial basal cell epithelioma, interconnections between tumor‐cell nests were likely to exist.It is suggested that this method may fill a gap between macroscopic and microscopic findings, and that it is of considerable use for detecting invasive changes, for determining interrelationships between tumors and skin appendages, and for deciding ranges of surgical operations.
ULTRASTRUCTURE OF CUTANEOUS LESIONS IN LUPUS ERYTHEMATOSUS: A COMPARISON BETWEEN THE CUTANEOUS AND SYSTEMIC TYPES
Authors: Ogawa Kimiko 
Published: April 2015

ABSTRACTThe author investigated the basal lamina of cutaneous lesions in lupus erythematosus (LE) at the dermal‐epidermal junction ultrastructurally, and discussed the differences between cutaneous LE (CLE) and systemic LE (SLE) and also among the types of cutaneous eruptions.The findings included micro‐depression of the cytoplasm of basal cells, widening of the zona lucida, dissociation between anchoring filaments and the basal lamina, separation of the lamina from epidermal cells, focal defects and bifurcations of the basal lamina, and newly formed fine basal laminae at the original sites adjacent to the basal cells. The basal lamina deeply invaded the dermis with branching or multiplication which looked like loops or labyrinths and was thickened. These changes were all considered to be antecedents to the multiplication of the basal lamina. The basal lamina was multiple and sometimes more than five‐layered. The laminae of the latter are thought to be specific to LE.The distances from the outer leaflet of the plasma membranes of basal cells to the deepest points of the multilayered basal laminae in the dermis were measured. They were regarded as an index of the degree of the multiplication of the lamina. Their mean value was significantly greater in CLE than in SLE. This fact indicated that the multiplication of the basal lamina in CLE was higher than in SLE. There were no significant differences among the types of cutaneous eruptions.The basal lamina is known to be formed beneath half‐desmosomes without participation of the dermis. Thus multiplication is thought to be a secondary phenomenon following repeated damage to the epidermal cells.
STAPHYLOCOCCI ISOLATED FROM PYODERMAS AND THEIR SENSITIVITIES TO ANTIBIOTICS
Authors: Dekio Satoshi , Takagaki Kenji , Yamasaki Reiko , Hamanaka Kazuko , Ikeda Sanae , Onimura Kentaro 
Published: April 2015

ABSTRACTWe investigated species and strains of bacteria, chiefly Staphylococci, isolated from the lesions of several pyodermas and their sensitivities to several antibiotics during 1975–1979. In furuncle and impetigo contagiosa, Staphylococcus aureus comprised most of the isolated bacteria. In folliculitis, Staphylococcus aureus and Staphylococcus epidermidis occurred at about equal frequency among the isolated bacteria, and in infectious atheroma, more than half of the bacterial isolates were Staphylococcus epidermidis. The sensitivities of the strains of Staphylococcus aureus were in the order of CEX, GM > CP > TC > ABPC, AMPC > EM, and those of Staphylococcus epidermidis were in the order of CEX, GM > CP > ABPC, AMPC > EM, TC. The sensitivities of the strains of Staphylococcus aureus were generally lower than those of Staphylococcus epidermidis.
URTICARIA PIGMENTOSA IN PATIENTS CLOSELY RELATED BY BIRTH
Authors: Aoki Kiyoko , Suzuki Hiroyuki 
Published: April 2015

ABSTRACTAn occurrence of urticaria pigmentosa in two sisters was studied. The clinical characteristics included the rare location of the lesions in both sisters and an onset after rubeola in the elder sister. Serum histamine levels throughout the family were markedly higher than normal.Investigations into the family medical history failed to disclose clear evidence of any hereditary factor. Perhaps many triggers in addition to hereditary factors are needed to initiate urticaria pigmentosa.
AXILLARY PAGET'S DISEASE
Authors: Imai Seiji , Nitto Heita , Imai Reiko 
Published: April 2015

ABSTRACTA case of axillary Paget's disease in a female was presented. Histopathological study revealed various types of apocrine poral duct tumors showing benignancy, premalignancy or malignancy, and Paget's cells in the epidermis as well as in the latter lesions. Relationship between Paget's cell and the malignant cell was discussed.
ULTRASTRUCTURAL STUDIES OF VITILIGO WITH INFLAMMATORY RAISED BORDERS
Authors: Ishii Masamitsu , Hamada Toshio 
Published: April 2015

ABSTRACTUltrastructural studies of vitiligo with inflammatory raised borders were performed. A 41‐year‐old Japanese female had developed vitiligo on the back and cervical region about six months previously and it gradually increased. Slightly elevated red borders were observed on the periphery of some of the vitiligo lesions. Electron microscopic findings showed a few melanocytes with decreased and irregular melanosomes in the basal layer and dissociation of keratinocytes in the spinous layers. Melanosomes in keratinocytes were markedly decreased. Lymphocytes were frequently observed intraepidermally, and the process by which lymphocytes passed to the basal lamina from the dermis was also observed. Lymphocytes were seen in apposition to some melanocytes in the basal layer. These melanocytes, as a result, may degenerate. The formation of many vacuoles in the cytoplasm was observed. Many typical Langerhans cells with many racket bodies were observed in the upper spinous layers. On the other hand, in the basal layer, Langerhans cells with only a few racket bodies were generally observed. These Langerhans cells were also frequently in contact with lymphocytes.The cause of this inflammatory reaction with a raised border is not known. However, these ultrastructural findings firmly suggested a relationship to some type of immunological mechanism for this occurrence of vitiligo.
EXACERBATION OF PSORIASIS INDUCED BY INDOMETHACIN
Authors: Katayama Hiroshi , Kawada Akihiro 
Published: April 2015

ABSTRACTIn an attempt to clarify the role of prostaglandins in the pathogenesis of psoriasis, indomethacin, a potent inhibitor of prostaglandin synthesis, was administered orally to 6 psoriatic patients and topically to 5 patients. In all cases remarkable exacerbation of pre‐existing lesions was observed within 1 to 4 weeks. The possible mechanism of the clinical exacerbation was discussed with particular reference to arachidonic acid‐prostaglandin system.
ISOLATION AND CHARACTERIZATION OF THE MEMBRANEOUS FRACTION IN HUMAN STRATUM CORNEUM
Authors: Manabe Motomu , Hirotani Tetsuya , Negi Makoto , Hattori Michihiro , Ogawa Hideoki 
Published: April 2015

ABSTRACTMembraneous fractions were isolated from human stratum corneum. The fractions obtained after frequent reextraction with urea and 2 ME at pH 9.0 (A), and further digestion of (A) by trypsin (B) were compared using electron microscopy and amino acid analysis. Low molecular weight proteins or amino peptides were solubilized from fraction (A) following digestion with trypsin, chymotrypsin and pronase P. The amounts of solubilized material showed a plateau after 30 minutes of digestion with trypsin. A small fraction of contaminants thought to be the fibrous and interfibrous materials were still observed in Fraction (A) by electron‐microscopy and SDS gel electrophoresis, but no remarkable contaminants were observed in Fraction (B), which was composed of trilaminar membraneous parts including the marginal band. Fraction (A) contained 43 half cystine residues per 1000 amino acid residues, whereas Fraction (B) contained 81 half cystine residues per 1000 amino acid residues.
TWO FORMS OF ADENOSINE DEAMINASE IN PIG EPIDERMIS
Authors: Iizuka Hajime , Koizumi Hiroko , Kamigaki Katsuko , Aoyagi Takashi , Miura Yusho 
Published: April 2015

ABSTRACTMolecular heterogeneity of adenosine deaminase in pig epidermis is reported. Gel filtration of pig skin extracts with Sephadex G‐150 revealed the existence of two forms of adenosine deaminase. Molecular weights of the enzymes were around 30,000–40,000 and 300,000–350,000 respectively. Several catalytic properties of these two adenosine deaminases were quite similar: Km value, substrate specificity, pH activity profile, and the effect of coformycin, a strong inhibitor of adenosine deaminase.
DNA SYNTHESIS IN CULTURED EPIDERMAL CELLS FROM PSORIATIC AND NORMAL SKIN
Authors: Katayama Hiroshi , Hori Hiroyuki , Kawada Akihiro 
Published: April 2015

ABSTRACTWhile it is generally accepted that psoriatic epidermis in vivo shows an increase in the number of DNA‐synthesizing cells and mitoses, the published data from in vitro studies disagree as to whether or not psoriatic epidermal cells in vitro are more proliferative than normal epidermal cells. In this study, DNA synthesis was measured autoradiographically using cultured epidermal cells from both the involved and uninvolved skin of 8 psoriatic patients as well as from 8 normal individuals. Our results showed no statistically significant differences in DNA synthesis among the three groups.
A CASE OF COLD URTICARIA DUE TO A SERUM FACTOR BELONGING TO THE IGE CLASS
Authors: Akiyama Tomio , Ushijima Nobuo , Anan Sadao , Takahashi Isamu , Yoshida Hikotaro 
Published: April 2015

ABSTRACTA case of cold urticaria showing a positive Prausnitz‐Küstner (P‐K) reaction was studied. The results obtained were as follows: 1) the P‐K reaction was positive, 2) skin fixation time was 21 days, 3) the P‐K reaction could be blocked by heating the serum for 2 hours at 56°C, and 4) the P‐K reaction became negative after adsorption of IgE from the serum. These data suggest that the serum factor responsible for cold urticaria in this case belongs to the IgE class. The effect of histamine liberator 48/80, histological examination of the skin and results of the estimation of serum histamine concentration by a fluorometric method all suggest that this wheal formation is due to a histamine release from mast cells in the skin.
INCIDENCE OF LUPUS ERYTHEMATOSUS IN NIGERIA
Authors: Somorin A.O. , Adesugba J. 
Published: April 2015

ABSTRACTA retrospective review of twelve Nigerian patients treated at the University Teaching Hospital, Lagos, Nigeria for Lupus erythematosus within a ten‐year period is presented. Both the masquerading clinical spectrum and prognosis of this disease are similar to those encountered in cold countries of the world. This paper highlights hypothetical explanations for the rarity of this disease in Nigeria, while it draws attention to tropical clinical simulators of the disease.
CYCLIC AMP‐DEPENDENT PROTEIN PHOSPHORYLATION OF PIG EPIDERMAL HOMOGENATES
Authors: Aoyagi Takashi , Umeda Katsuko , Kato Naoko , Koizumi Hiroko , Nemoto Osamu , Kobayashi Hitoshi , Miura Yusho 
Published: April 2015

ABSTRACTThere are four independent stimulators (epinephrine, histamine, adenosine and prostaglandin E) of the adenylate cyclase receptor on the epidermal cell surface, all of which increase intracellular cyclic AMP. These stimulators and db‐cAMP inhibit epidermal cell proliferation and promote epidermal cell differentiation, although conflicting effects have been reported in pure epidermal cell culture systems. In order to elucidate the physiological roles of cyclic AMP in epidermal cells, endogenous protein phosphorylation of pig epidermal homogenates was studied. The autoradiographic analysis by SDS‐PAGE demonstrated that cyclic AMP (10 μM) stimulated the phosphorylation of several epidermal proteins. One group of these endogenous substrates were keratinous proteins. These phosphoproteins were dephosphorylated by chelating Mg2+ with EDTA. The addition of unlabeled ATP diminished the intensity of phosphoprotein bands.These results indicate that pig epidermal homogenates have cyclic AMP‐dependent protein kinase and that some endogenous substrates were keratinous proteins.
LUPUS BAND TEST IN NEW ZEALAND MICE AND MRL MICE
Authors: Furukawa Fukumi , Hamashima Yoshihiro 
Published: April 2015

ABSTRACTThe results of the lupus band test (LBT) in New Zealand mice and MRL mice were reported. In NZB × NZW (B/W) F1 mice, the deposition of immunoglobulin at the dermal‐epidermal junction (DEJ) was first observed in some mice at the age of six months and all were positive by the age of twelve months. A regional comparative study of LBT showed that the tail had the highest incidence. The deposition was in a granular or confluent pattern. In some of MRL‐lpr/lpr mice, LBT was positive at the age of two months and showed 90–100% positivity at over five months. The deposition revealed a confluent pattern with focal variation in density. In both strains the deposition of Ig G1 at DEJ was most frequently demonstrated and circulating anti‐basement membrane antibodies were not observed.We discussed the similarities and differences between murine LBT and human LBT.