bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and Characterization of Near‐Infrared Absorbing Water Soluble Squaraines and Study of their Photodynamic Effects in DLA Live Cells
Authors: Shafeekh Kulathinte M. , Soumya Mohanannair S. , Rahim Moochikkadavath A. , Abraham Annie , Das Suresh 
Published: January 2014

AbstractHere, we report the synthesis, photophysical properties and photodynamic effects in DLA live cells of three water soluble squaraine dyes, viz. bisbenzothiazolium squaraine dyes SQMI and SQDI with iodine in one and both benzothiazolium units, respectively, and an unsymmetrical squaraine dye ASQI containing iodinated benzothiazolium and aniline substituents. The diiodinated SQDI showed an anomalous trend in both fluorescence and triplet quantum yields over the monoiodinated SQMI, with SQDI showing higher fluorescence and lower triplet quantum yields compared to SQMI. Nanosecond laser flash photolysis of SQDI and SQMI indicated the formation of triplet excited states with quantum yield of 0.19 and 0.26, respectively. On photoirradiation, both the SQDI and SQMI generate singlet oxygen and it was observed that both dyes undergoing oxidation reactions with the singlet oxygen generated. ASQI which exhibited a lower triplet quantum yield of 0.06 was, however, stable and did not react with the singlet oxygen generated. In vitro cytotoxicity studies of these dyes in DLA live cells were performed using Trypan blue dye exclusion method and it reflect an order of cytotoxicity of SQDI>SQMI>ASQI. Intracellular generation of the ROS was confirmed by dichlorofluorescein assay after the in vitro PDT.
Shade Provision for UV Minimization: A Review
Authors: Parisi Alfio V. , Turnbull David J. 
Published: January 2014

AbstractMinimizing exposure to ultraviolet (UV) radiation is an essential component of skin cancer prevention. Providing and using natural and built shade is an effective protection measure against harmful UV. This article describes the factors that must be addressed to ensure quality, effective, well‐designed shade and recommends best practice approaches to improving the protection factor (PF) of shade structures. It identifies examples of interventions to increase shade availability and use, and examples of effective shade based on measured protection factors or measured reductions in UV exposures. Finally, this article considers examples of best practice for undertaking shade audits. The article is based on refereed articles and reviews, reports, conference papers and shade practice and policies from reports and on web sites. Articles for the Australian setting are considered first, followed by those in an international setting.
More on Idealism and Skepticism
Authors: Brueckner Anthony 
Published: January 2014

AbstractIn “Idealism and Skepticism: A Reply to Brueckner”, Stephen Puryear maintains that Berkeley holds at most that ideas that “count as real things” as opposed to chimeras (e.g., my idea of the Eiffel Tower) must satisfy a criterion of intra‐mind coherence. On this reading of Berkeley, my claim that a form of external‐world epistemological skepticism can be constructed within Berkeley's metaphysical system cannot get off the ground. I respond here.
The rates, perceptions, and willingness of men who have sex with men to donate blood
Authors: Liszewski Walter , Becerril Jordan , Terndrup Christopher , West Nathan , Lavin Bridget C. , Schieffler Danny , Van Sickels Nicholas 
Published: January 2014

BackgroundSince 1983 in the United States, any man who has had sex with another man (MSM) at any time since 1977 has been deferred from donating blood for life. Although there has been a push to change the deferral, there is a paucity of information on both the rates of MSM blood donation and the willingness of MSMs to donate if the deferral were changed.Study Design and MethodsA 15‐question survey was given at two lesbian, gay, bisexual, and transgender festivals in Chicago and New Orleans. Participants were asked about a previous history of blood donation and whether they would be willing to donate were the lifetime deferral changed. Participants were also asked to determine whether it was safe for hypothetical MSMs with varying sexual practices to donate blood and whether they believed that it was safe for them to donate their own blood.ResultsOur study found that 42.0% of all participants had not complied with the deferral policy and have donated blood at least once, with a mean number of donations of 4.84. Additionally, 85.9% of participants would be willing to donate blood if the deferral were changed.ConclusionDespite the lifetime deferral, many MSMs have previously donated blood, and many more are willing to donate. Given this, along with the safe implementation of temporary deferral policies in other nations, the United States should consider adopting a temporary deferral policy for MSMs.
The success of our patient blood management program depended on an institution‐wide change in transfusion practices
Authors: Oliver James C. , Griffin Russell L. , Hannon Timothy , Marques Marisa B. 
Published: January 2014

BackgroundPatient blood management (PBM) programs seek to optimize the utilization of blood components. Since our institution's program started, the annual number of red blood cell (RBC) units transfused has decreased by 27% overall.Study Design and MethodsWe collected data for 6 months in 2007 (pre‐PBM) compared with the same months in 2011 (post‐PBM) to determine which changes in practice decreased RBC utilization. Indications for transfusion of nonsurgical patients were collected from the electronic medical records, while surgical indications were assigned to the admitting physician's specialty.ResultsPre‐PBM, we transfused 19,888 RBC units for a mean of 0.96 units per patient discharged, compared with 14,472 post‐PBM, for a mean of 0.55 units per discharge. This represents a 43% reduction in RBC units transfused per patient discharged. While transfusion episodes decreased only slightly from 9519 to 9261, the success can be explained by the overall reduction in mean number of units per transfusion from 2 to 1.5 (p < 0.0001). Pre‐PBM, 22 and 48% of patients received 1 or 2 units of RBCs per transfusion episode, respectively, while in 2011, the percentages were 51 and 33%, respectively (p < 0.0001). The mean number of RBC units per transfusion decreased significantly for approximately 50% of the indications.ConclusionOur success was achieved through hospital‐wide physician buy‐in toward a restrictive transfusion approach. We hope to encourage others to consider PBM for improved patient outcomes and blood conservation.
The Surgeon General Report on Smoking and Health 50 Years Later: Breast Cancer and the Cost of Increasing Caution
Authors: Glantz Stanton A. , Johnson Kenneth C. 
Published: January 2014

AbstractDespite the Surgeon General's strong track record and the rapidly expanding body of solid scientific work demonstrating that smoking caused a wide range of diseases, the decision making process for concluding “causality” in Surgeon General reports has become increasingly cautious and defensive. Whereas, the 1964 report did not conclude that smoking caused heart disease, it recommended that “from the public health viewpoint [one should] assume that the established association has causative meaning rather than to suspend judgment until no uncertainty remains,” the de facto practice has become to do just the opposite. In particular, the 2004 report reached an affirmative negative conclusion that active smoking did not cause breast cancer and the 2006 report on passive smoking only found the link “suggestive.” In contrast, in 2005 the California EPA found both active and passive smoking caused breast cancer in younger women. The evidence has continued to strengthen since 2005: there are now 12 large cohort studies that consistently demonstrate a dose–response relationship with smoking before first birth and increased breast cancer risk. The Surgeon General's increasing caution is preventing young women around the world from appreciating the risks that smoking and secondhand smoke pose for developing breast cancer. Cancer Epidemiol Biomarkers Prev; 23(1); 37–46. ©2014 AACR.
Comparison of Postprocessing Methods for the Calibration of 100-m Wind Ensemble Forecasts at Off- and Onshore Sites
Authors: Junk Constantin , von Bremen Lueder , Kühn Martin , Späth Stephan , Heinemann Detlev 
Published: January 2014

AbstractEnsemble forecasts are a valuable addition to deterministic wind forecasts since they allow the quantification of forecast uncertainties. To remove common deficiencies of ensemble forecasts such as biases and ensemble spread deficits, various postprocessing methods for the calibration of wind speed (univariate calibration) and wind vector (bivariate calibration) ensemble forecasts have been developed in recent years. The objective of this paper is to compare the performance of state-of-the-art calibration methods at distinct off- and onshore sites in central Europe. The aim is to identify calibration- and site-dependent improvements in forecast skill over uncalibrated 100-m ensemble forecasts from the ECMWF Ensemble Prediction System. The ensemble forecasts were evaluated at four onshore and three offshore measurement towers in central Europe at 100-m height for lead times up to 5 days. The results show that the recursive and adaptive wind vector calibration (AUV) outperforms calibration methods such as univariate ensemble model output statistics (EMOS), bivariate EMOS, variance deficit calibration, and ensemble copula coupling in terms of the root-mean-square error and continuous ranked probability score at almost all sites. It was found that exponential downweighting of past measurements in AUV contributes to higher forecast skill since similar downweighting approaches in the other calibration methods improved forecast skill. Proposing a bidimensional bias correction in bivariate EMOS similar to the approach taken in AUV yields bivariate EMOS skill at onshore sites that is similar to AUV skill. Deterministic and probabilistic improvements are usually much lower at offshore sites and increase with increasing complexity of the site characteristics since systematic forecast errors and ensemble underdispersion are larger at high-roughness sites.
An Empirical Relation between U.S. Tornado Activity and Monthly Environmental Parameters
Authors: Tippett Michael K. , Sobel Adam H. , Camargo Suzana J. , Allen John T. 
Published: January 2014

Abstract
In previous work the authors demonstrated an empirical relation, in the form of an index, between U.S. monthly tornado activity and monthly averaged environmental parameters. Here a detailed comparison is made between the index and reported tornado activity. The index is a function of two environmental parameters taken from the North American Regional Reanalysis: convective precipitation (cPrcp) and storm relative helicity (SRH). Additional environmental parameters are considered for inclusion in the index, among them convective available potential energy, but their inclusion does not significantly improve the overall climatological performance of the index. The aggregate climatological dependence of reported monthly U.S. tornado numbers on cPrcp and SRH is well described by the index, although it fails to capture nonsupercell and cool season tornadoes. The contributions of the two environmental parameters to the index annual cycle and spatial distribution are examined with the seasonality of cPrcp (maximum during summer) relative to SRH (maximum in winter) accounting for the index peak value in May. The spatial distribution of SRH establishes the central U.S. “tornado alley” of the index, while the spatial distribution of cPrcp enhances index values in the South and Southeast and suppresses them west of the Rockies and over elevation. At the scale of the NOAA climate regions, the largest deficiency of the index climatology occurs over the central region where the index peak in spring is too low and where the late summer drop-off in the reported number of tornadoes is poorly captured. This index deficiency is related to its sensitivity to SRH, and increasing the index sensitivity to SRH improves the representation of the annual cycle in this region. The ability of the index to represent the interannual variability of the monthly number of U.S. tornadoes can be ascribed during most times of the year to interannual variations of cPrcp rather than of SRH. However, both factors are important during the peak spring period. The index shows some skill in representing the interannual variability of monthly tornado numbers at the scale of NOAA climate regions.
Contributing Factors to the Recent High Level of Accumulated Cyclone Energy (ACE) and Power Dissipation Index (PDI) in the North Atlantic*
Authors: Murakami Hiroyuki , Li Tim , Hsu Pang-Chi 
Published: January 2014

Abstract
In recent decades, tropical cyclone (TC) activity in the North Atlantic has shown a marked positive anomaly in genesis number, mean lifespan, number of intense hurricanes, and mean maximum intensity. The accumulated cyclone energy (ACE), which is defined as the sum of the square of the maximum surface wind velocity throughout the lifetime of a TC, is one of the measures that can be used to synthesize these factors. Similar to the ACE, the power dissipation index (PDI), which is defined as the integrated third power of maximum surface wind velocity, has also been used to describe TC activity. The basin-total ACE and PDI for the North Atlantic have also followed a large positive anomaly during the period 1995–2012; however, the relative importance of factors such as TC genesis number, TC track property (e.g., duration and lifespan), and TC intensity remains unclear in terms of their contribution to the positive anomalies in ACE and PDI. This study uses a new empirical statistical approach to analyze the TC data and finds that the increase in the TC genesis number is primarily responsible for the positive anomalies in ACE and PDI. Other factors, such as TC track property and TC intensity, appear to be minor influences.
Cold Tongue and Warm Pool ENSO Events in CMIP5: Mean State and Future Projections
Authors: Taschetto Andréa S. , Gupta Alexander Sen , Jourdain Nicolas C. , Santoso Agus , Ummenhofer Caroline C. , England Matthew H. 
Published: January 2014

Abstract
The representation of the El Niño–Southern Oscillation (ENSO) under historical forcing and future projections is analyzed in 34 models from the Coupled Model Intercomparison Project phase 5 (CMIP5). Most models realistically simulate the observed intensity and location of maximum sea surface temperature (SST) anomalies during ENSO events. However, there exist systematic biases in the westward extent of ENSO-related SST anomalies, driven by unrealistic westward displacement and enhancement of the equatorial wind stress in the western Pacific. Almost all CMIP5 models capture the observed asymmetry in magnitude between the warm and cold events (i.e., El Niños are stronger than La Niñas) and between the two types of El Niños: that is, cold tongue (CT) El Niños are stronger than warm pool (WP) El Niños. However, most models fail to reproduce the asymmetry between the two types of La Niñas, with CT stronger than WP events, which is opposite to observations. Most models capture the observed peak in ENSO amplitude around December; however, the seasonal evolution of ENSO has a large range of behavior across the models. The CMIP5 models generally reproduce the duration of CT El Niños but have biases in the evolution of the other types of events. The evolution of WP El Niños suggests that the decay of this event occurs through heat content discharge in the models rather than the advection of SST via anomalous zonal currents, as seems to occur in observations. No consistent changes are seen across the models in the location and magnitude of maximum SST anomalies, frequency, or temporal evolution of these events in a warmer world.
Orographic Effects of the Tibetan Plateau on the East Asian Summer Monsoon: An Energetic Perspective
Authors: Chen Jinqiang , Bordoni Simona 
Published: January 2014

AbstractThis paper investigates the dynamical processes through which the Tibetan Plateau (TP) influences the East Asian summer monsoon (EASM) within the framework of the moist static energy (MSE) budget, using both observations and atmospheric general circulation model (AGCM) simulations. The focus is on the most prominent feature of the EASM, the so-called meiyu–baiu (MB), which is characterized by a well-defined, southwest–northeast elongated quasi-stationary rainfall band, spanning from eastern China to Japan and into the northwestern Pacific Ocean between mid-June and mid-July.Observational analyses of the MSE budget of the MB front indicate that horizontal advection of moist enthalpy, and primarily of dry enthalpy, sustains the front in a region of otherwise negative net energy input into the atmospheric column. A decomposition of the horizontal dry enthalpy advection into mean, transient, and stationary eddy fluxes identifies the longitudinal thermal gradient due to zonal asymmetries and the meridional stationary eddy velocity as the most influential factors determining the pattern of horizontal moist enthalpy advection. Numerical simulations in which the TP is either retained or removed show that the TP influences the stationary enthalpy flux, and hence the MB front, primarily by changing the meridional stationary eddy velocity, with reinforced southerly wind over the MB region and northerly wind to its north. Changes in the longitudinal thermal gradient are mainly confined to the near downstream of the TP, with the resulting changes in zonal warm air advection having a lesser impact on the rainfall in the extended MB region.
Characterization of Toxoplasma gondii subtelomeric-like regions: identification of a long-range compositional bias that is also associated with gene-poor regions
Authors: Dalmasso María C , Carmona Santiago J , Angel Sergio O , Agüero Fernán 
Published: January 2014

Abstract

Background
Chromosome ends are composed of telomeric repeats and subtelomeric regions, which are patchworks of genes interspersed with repeated elements. Although chromosome ends display similar arrangements in different species, their sequences are highly divergent. In addition, these regions display a particular nucleosomal composition and bind specific factors, therefore producing a special kind of heterochromatin. Using data from currently available draft genomes we have characterized these putative Telomeric Associated Sequences in Toxoplasma gondii.

Results
An all-vs-all pairwise comparison of T. gondii assembled chromosomes revealed the presence of conserved regions of ∼ 30 Kb located near the ends of 9 of the 14 chromosomes of the genome of the ME49 strain. Sequence similarity among these regions is ∼ 70%, and they are also highly conserved in the GT1 and VEG strains. However, they are unique to Toxoplasma with no detectable similarity in other Apicomplexan parasites. The internal structure of these sequences consists of 3 repetitive regions separated by high-complexity sequences without annotated genes, except for a gene from the Toxoplasma Specific Family. ChIP-qPCR experiments showed that nucleosomes associated to these sequences are enriched in histone H4 monomethylated at K20 (H4K20me1), and the histone variant H2A.X, suggesting that they are silenced sequences (heterochromatin). A detailed characterization of the base composition of these sequences, led us to identify a strong long-range compositional bias, which was similar to that observed in other genomic silenced fragments such as those containing centromeric sequences, and was negatively correlated to gene density.

Conclusions
We identified and characterized a region present in most Toxoplasma assembled chromosomes. Based on their location, sequence features, and nucleosomal markers we propose that these might be part of subtelomeric regions of T. gondii. The identified regions display a unique trinucleotide compositional bias, which is shared (despite the lack of any detectable sequence similarity) with other silenced sequences, such as those making up the chromosome centromeres. We also identified other genomic regions with this compositional bias (but no detectable sequence similarity) that might be functionally similar.
Unexplained diarrhoea in HIV-1 infected individuals
Authors: Oude Munnink Bas B , Canuti Marta , Deijs Martin , de Vries Michel , Jebbink Maarten F , Rebers Sjoerd , Molenkamp Richard , van Hemert Formijn J , Chung Kevin , Cotten Matthew , Snijders Fransje , Sol Cees JA , van der Hoek Lia 
Published: January 2014

Abstract

Background
Gastrointestinal symptoms, in particular diarrhoea, are common in non-treated HIV-1 infected individuals. Although various enteric pathogens have been implicated, the aetiology of diarrhoea remains unexplained in a large proportion of HIV-1 infected patients. Our aim is to identify the cause of diarrhoea for patients that remain negative in routine diagnostics.

Methods
In this study stool samples of 196 HIV-1 infected persons, including 29 persons with diarrhoea, were examined for enteropathogens and HIV-1. A search for unknown and unexpected viruses was performed using virus discovery cDNA-AFLP combined with Roche-454 sequencing (VIDISCA-454).

Results
HIV-1 RNA was detected in stool of 19 patients with diarrhoea (66%) compared to 75 patients (45%) without diarrhoea. In 19 of the 29 diarrhoea cases a known enteropathogen could be identified (66%). Next to these known causative agents, a range of recently identified viruses was identified via VIDISCA-454: cosavirus, Aichi virus, human gyrovirus, and non-A non-B hepatitis virus. Moreover, a novel virus was detected which was named immunodeficiency-associated stool virus (IASvirus). However, PCR based screening for these viruses showed that none of these novel viruses was associated with diarrhoea. Notably, among the 34% enteropathogen-negative cases, HIV-1 RNA shedding in stool was more frequently observed (80%) compared to enteropathogen-positive cases (47%), indicating that HIV-1 itself is the most likely candidate to be involved in diarrhoea.

Conclusion
Unexplained diarrhoea in HIV-1 infected patients is probably not caused by recently described or previously unknown pathogens, but it is more likely that HIV-1 itself plays a role in intestinal mucosal abnormalities which leads to diarrhoea.