bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Mechanism of the Inhibitory Effects of Antimalarials on UV‐induced Skin Inflammation in Mice
Authors: Yoshioka Akira , Miyachi Yoshiki , Imamura Sadao 
Published: April 2015

AbstractIn order to elucidate the mechanism of UV‐induced skin inflammation, the effects of antimalarials, inhibitors of phospholipase A2 and membrane phospholipid methylation, on UV‐induced ear swelling response (ESR) was examined. Antimalarials inhibited ESR induced by 8‐methoxypsoralen (0.5%) plus UVA irradiation (PUVA treatment) or UVB irradiation. However, they did not inhibit ESR induced by the application of arachidonic acid (AA). Although it has been reported that PUVA treatment and UVB irradiation may work through different AA pathways, it seems likely that both PUVA and UVB may affect the initial cellular metabolism of the inflammatory process.
The BamHI Restriction Fragment Length Polymorphism Detected in the c‐Ha‐ras Locus of Human Skin Tumors
Authors: Murayama Minoru , Mita Takashi , Suenaga Yoshinori , Nishio Kazukata 
Published: April 2015

AbstractBy means of Southern blot hybridization, 13 human primary skin tumor tissues were analyzed for activated forms of three cellular oncogenes, c‐Ha‐ras, c‐myc, and c‐erbB. In the c‐Ha‐ras oncogene, a BamHI restriction fragment length polymorphism was found among human skin tumors, but it is not clear whether it is related to the malignancy of tumors selected for this study. We were not able to detect the activated forms of the three oncogenes used in this study.
The Antibiotic Susceptibility of Propionibacterium acnes: A 15‐year Bacteriological Study and Retrospective Evaluation
Authors: Kurokawa Ichiro , Nishijima Setsuko , Asada Yasuo 
Published: April 2015

AbstractPropionibacterium acnes strains were isolated from the comedones of 46 acne patients, and their susceptibilities to penicillin G, ampicillin, erythromycin, clindamycin, tetracycline, doxycycline, minocycline, cephalexin and gentamycin were studied by examining their minimum inhibitory concentrations. Two additional standard strains were included in the study. In the results, differing antibiotic susceptibilities were found, and P. acnes strains were the most sensitive to erythromycin and clindamycin, followed by ampicillin and minocycline. The highest levels of resistance were observed against clindamycin, erythromycin and tetracycline. Non‐resistant strains were detected only against minocycline. A comparison of these results with others obtained by similar studies in our department during the last 15 years showed a progressive tendency toward antibiotic resistance for Propionibacterium acnes, probably related to the systemic administration of antibiotics for the treatment of bacterial infections other than acne.
Desmoplastic Malignant Melanoma
Authors: Nakagawa Hidemi , Lee Ai Young , Kikuchi Kanako , Kurose Nobuyuki , Kawashima Makoto , Nogita Toshitatsu , Ishibashi Yasumasa 
Published: April 2015

AbstractA 86‐year‐old Japanese woman presented with an amelanotic tumor that had arisen on an antecedent pigmented lesion (acral lentiginous melanoma) on the lateral side of her right foot. The bulk of the tumor was composed of atypical spidle cells in a fibrous or myxoid matrix, pathognomonic of desmoplastic malignant melanoma. Immunohistochemically, neoplastic cells were positive for S‐100 protein and neuron specific enolase. Ultrastructurally, the majority of spindle cells showed Schwannian features and contained no melanosomes. Occasional atypical cells, however, contained ellipsoidal organelles with subtle cross internal striations but did not show Schwannian features. Our findings lend further support to the hypothesis that the desmoplasia in desmoplastic malignant melanoma is the result of acquired fibrogenetic capacities of dedifferentiated melanoma cells.
Squamous Cell Carcinoma Developing in Thermal Keratosis
Authors: Yamaji Kazuhiko , Haniuda Kumiko , Shindo Yasuko , Saida Toshiaki 
Published: April 2015

AbstractTwo cases of squamous cell carcinoma (SCC), both of them developed from thermal keratosis arising in areas of erythema ab igne (EAI) are reported. The lesions were located on the legs of two elderly Japanese women who had histories of chronic exposure to heat from a “kotatsu”, a type of leg‐warmer widely used in Japan. In one case, metastasis to regional lymph nodes occurred. It is emphasized that keratotic lesions arising in an area of EAI should be excised or biopsied because of the possibility of metastasizing SCC developing from them.
Comparison of the Relative Therapeutic Efficacy of 7‐Methyl Pyridopsoralen and 8‐Methoxypsoralen in Photochemotherapy in Psoriasis Treatment
Authors: Takashima Akira , Sunohara Akiyo , Mizuno Nobuyuki 
Published: April 2015

AbstractA newly‐synthesized, monofunctional psoralen derivative, 7‐methyl pyrido (3,4‐C) psoralen (MPP) was compared with 8‐methoxypsoralen (8MOP) with respect to their therapeutic efficacy in photochemotherapy of psoriasis. Psoriatic lesions of six patients were treated with topical application of MPP plus UVA (MPP PUVA) or with 8MOP plus UVA (8MOP PUVA). The UVA doses used in each treatment were 7.5 or 10 J/cm2 with MPP and from 1.2 to 3.6 J/cm2 with 8MOP. In every patient, marked improvement was observed after 2 to 6 treatments with MPP PUVA or 8MOP PUVA. Three patients showed clearance of each psoriatic lesion treated 9 to 17 times with MPP or 8MOP PUVA. Althought MPP required much higher UVA doses than 8MOP, MPP PUVA was as effective as 8MOP PUVA in treating psoriasis. When irradiating with identical doses of UVA, MPP PUVA appeared to be less active than 8MOP PUVA. None of the patients developed any severe dermatitis reactions during 20 exposures to MPP PUVA, indicating that the probability of inducing allergic contact and photocontact dermatitis may be extremely low. Erythemogenic and pigmentogenic activities of MPP and 8MOP were also compared. The data demonstrated that 8MOP is more than 8 times as effective as MPP for both activities. With the UV doses used in this study, however, every patient produced marked pigmentation after MPP PUVA therapy. Finally, the UVA dose‐dependency of MPP PUVA was studied with an additional patient. Both therapeutic and pigmentogenic effects increased as a function of the UVA dose; it appeared impossible to clear psoriasis without producing pigmentation.
Characteristics of Prolidase and Prolinase in Prolidase‐deficient Patients with Some Preliminary Studies of Their Role in Skin
Authors: Oono Takashi , Arata Jirô 
Published: April 2015

Abstract
The prolidase (Pd) and prolinase (Pn) activities of cultured skin fibroblasts derived from two prolidase‐deficient sisters, the elder with typical clinical manifestations [symptom (+)] and the younger with only slight clinical manifestations [symptom (–)] were examined biochemically. Pd activity against several substrates other than Gly‐Pro were present to some degree in both sisters. There were no detectable differences in Pd activity between the symptom (+) patient and the symptom (–) sister. Pn activity seemed to be increased in both. The lower Pn activity found against Pro‐Gly as compared with those against other substrates indicates that Pro‐Gly, which has been used for Pn assays in most previous reports, may not be the best substrate for this test. Pd derived from control fibroblasts was activated by Mn2+ against all substrates tested in this experiment. Cu2+, Hg2+, Cd2+ and Zn2+ remarkably inhibited enzyme activity, Co2+ slightly inhibited it, and neither Mg2+ nor Fe2+ had any remarkable effect. The Pd derived from the prolidase‐deficient patients was also activated by Mn2+. This Pd seemed to be more inhibited by Co2+ than was the control. However, we found no remarkable differences between the two patients.
We also studied Pd and Pn activities in rat skin and blood during wound healing. Pd and Pn activities adjacent to the wound increased in parallel with fibroblast proliferation. Pd activity was also detected in an extract of newborn mouse epidermis.
Tissue Culture Study of Dermatofibrosarcoma Protuberans
Authors: Shindo Yasuko , Akiyama Junichi , Takase Yoshio 
Published: April 2015

AbstractWe cultured non‐epithelial cells derived from dermatofibrosarcoma protuberans (DFSP). The cells proliferated well, to the same degree as fibroblasts. DFSP cells, however, differed from fibroblasts in several aspects: 1) DFSP cells were bigger than fibroblasts, and grew in various directions, 2) acid phosphatase, and non‐specific esterase stained abundantly demonstrated in DFSP cells, 3) DFSP cells showed immunophagocytosis of Candida albicans and Staphylococcus aureus, and 4) DFSP cells had Fc and C3 receptors. DFSP cells are, therefore, considered to originate from histiocytes.
Natural Killer (NK) Cell Activity and NK‐related Cell Surface Markers in Patients with Atopic Dermatitis
Authors: Nomiyama Kazuhiko , Yamashita Uki , Nakamura Hiroshi , Suenaga Yoshinori , Nishio Kazukata 
Published: April 2015

AbstractNatural killer (NK) cell activity and NK‐related surface markers in peripheral blood mononuclear cells (PBMC) in thirty‐three patients with adult type atopic dermatitis (AD) were investigated. NK cell activity, detectable by cytolysis of erythroleukemic cell line K562 cells, was reduced in patient PBMC as compared with healthy controls. Imbalances in the lymphocyte populations reactable with the monoclonal antibodies, anti‐HNK‐1, and anti‐CD16 were found in the patient PBMC. Some positive correlations between these cell populations and the NK cell activities were recognized. In most cases, high levels of serum IgE were inversely correlated with NK cell activity. Depletion of the macrophages from patient PBMC had no effect on NK cell activities. These activities were enhanced by culture with β‐interferon for 18 hours, as were the NK cell activities of healthy controls. These results suggest that reduced numbers or imbalances in the lymphocyte population with NK cell activity, are responsible for the pathogenesis and the prognosis of adult type atopic dermatitis.
Morphological Identification of Langerhans Cells in Mouse Epidermal Cell Suspension by Scanning Electron Microscopy
Authors: Nakagawa Shojiro , Honma Takayoshi , Kumon Hiromi , Jinno Yoko , Bang Dongsik , Ueki Hiroaki , Ohmori Hiroyuki 
Published: April 2015

AbstractThe distribution of Iak antigens on suspended epidermal cells prepared from C3H/He mice by trypsinizing their ear skin was examined by the scanning immunoelectron microscopic method using antibody against the antigen and antibody bacteriophage T4 conjugates as a visual marker. Iak antigens were found to be distributed diffusely over a cell type with a relatively flat shape and a number of microvilli and ruffles. These cells are considered to be Langerhans cells. Significance of these findings is discussed.
Synergistic Effects of Hyperthermia and Peplomycin against Human Malignant Melanoma Xenografts
Authors: Yamada Kiyoshi , Nakagawa Hidemi , Etoh Takafumi , Ishibashi Yasumasa 
Published: April 2015

AbstractTwo types of human malignant melanoma were treated either with peplomycin alone, hyperthermia alone, or a combination of peplomycin and hyperthermia. The antitumor effect of combination therapy was evaluated by tumor growth curves, maximal suppression rate, and histopathology. A marked antitumor effect of combination therapy with peplomycin (5 mg/kg) and hyperthermia (43°C, 30 min) was obtained for both types of melanoma. Histopathologic findings revealed advanced central necrosis and pyknotic nuclei in the tumor cells. Although peplomycin alone (20 mg/kg, 6 times) had no antitumor effect on C24 melanoma, the peplomycin (5 mg/kg, 6 times) + heat group showed complete inhibition of the tumor growth. These results suggest that hyperthermia enhances the antitumor effect of peplomycin even against peplomycin‐resistant tumor cells.
Malignant Histiocytosis
Authors: Chen Minghua , Takemiya Masataka , Miyauchi Shunji , Murakami Shinji , Miki Yoshiharu 
Published: April 2015

AbstractThree patients with multiple skin nodules on the trunk and extremities showed hyperpyrexia, lymphadenopathy, pancytopenia, abnormal liver function, and terminal hemorrhagic diathesis. Histologically, the deep dermis and subcutaneous fat contained dense infiltrates of atypical erythropagocytic histiocytes and lymphoid cells. Autopsy also showed atypical histiocytes in the internal viscerae.
Microcystic Adnexal Carcinoma with Langerhans Cells
Authors: Takemiya Masataka , Shiraishi Satoshi , Saeki Norimichi , Ohtsuka Hisashi , Miki Yoshiharu 
Published: April 2015

AbstractA slowly growing, subcutaneous mass in the left eyebrow region of a 54‐year‐old Japanese woman showed numerous small epithelial cell nests surrounded by a dense desmoplastic stroma. The epithelial cell nests showed partially keratinizing cystic structures containing a small amount of glycogen. Immunoperoxidase staining for carcinoembryonic antigen was positive only in the lumina or on the ductal lining surface of tumor cells; staining for S‐100 protein was positive within almost all of the tumor nests. Birbeck granules indicating Langerhans cells were found within some of the cells.
Etretinate Treatment in Disseminated Porokeratosis
Authors: Danno Kiichiro , Yamamoto Masamitsu , Yokoo Tadashi , Ohta Miyuki , Ohno Sayoko 
Published: April 2015

AbstractA 77‐year‐old man with disseminated superficial porokeratosis of Mibelli (PM) over the whole body surface for 60 years was treated with oral etretinate (1 mg/kg/day) for nine months after resection of two lesions of Bowen's disease. Clinically, hyperkeratotic plaques were flattened after three months of treatment. Histological changes included disappearance of parakeratosis and residual but less stacked cornoid lamella. Etretinate may be the first choice of treatment in widespread porokeratosis.
Effects of 1α,25‐Dihydroxyvitamin D3 on the Transglutaminase Activity of Transformed Mouse Epidermal Cells in Culture
Authors: Lee Seung Churl , Ikai Kouichi , Ando Yoshihiro , Imamura Sadao 
Published: April 2015

AbstractInduction of the transglutaminase activity of a transformed mouse epidermal cell line (PAM 212 cells) by 1α,25‐dihydroxyvitamin D3 (1α,25‐(OH)2‐D3), the active form of vitamin D3, was investigated. Addition of 1α,25‐(OH)2‐D3 to a culture medium stimulated twice the transglutaminase activity at a concentration of 10−7 M, but vitamin D3, prostaglandin E1, E2, and F2a failed to show this induction. Phorbol 12‐o‐tetradecanoylphorbol‐13‐acetate (TPA) and ***dexamethasone also induced an increase in transglutaminase activity. Exposure to both 1α,25‐(OH)2‐D3 and retinoic acid caused remarkably synergistic effects on the induction of transglutaminase in the PAM 212 cells. In contrast, simultaneous addition of 1α,25‐(OH)2‐D3 and TPA was antagonistic and resulted in less than additive induction. Vitamin D3 also showed a similar but lesser effect. These results suggest that 1α,25‐(OH)2‐D3 induces the transglutaminase activity via mechanisms disparate from those of retinoic acid and modifies epidermal differentiation.
Glycosaminoglycan Content in the Media of Cultured Dermal Fibroblasts Derived from Burn Scar and Normal Skin
Authors: Nogami Reiko , Maekawa Yoshihiro , Kudo Shoichiro 
Published: April 2015

AbstractThe glycosaminoglycan (GAG) content of the extracellular matrix of burn scar in humans has been reported to differ from that of normal skin (1, 2). In order to investigate whether the GAG content altered as a result of functional changes in fibroblasts, the GAG content was determined in culture media of fibroblasts derived from growing burn scar, mature scar, and normal skin tissue. No statistical differences were observed in the population doubling‐times of scar and normal skin. Mature scar showed significantly higher values for all the concentrations of uronic acid, hexosamine, and sulfate measured in the glycosaminoglycan, as compared with normal skin values, and the concentrations from growing scar were slightly higher than those for normal skin. The above results may suggest an increase in glycosaminoglycan sulfate synthesis following the hyperplasia of the matrix in burn scar tissue.
Coexistence of Papular Mucinosis and Systemic Amyloidosis Associated with λ‐type IgD Paraproteinemia
Authors: Ishibashi Akira , Nakabayashi Kimimasa , Kukita Atsushi 
Published: April 2015

AbstractThe patient is an obese, 55‐year‐old woman. She noticed purpura at several sites when she was 49 years old. She visited our clinic with a chief complaint of exertional dyspnea at 51 years of age. Physical examination revealed localized edema in the left chest wall and lower abdomen with translucent papules in the center. Macroglossia, hemorrhagic macules, loss of axillar and pubic hairs, and goose‐egg sized swellings of submaxillar lymph nodes were also found. Laboratory data were within the normal range except an increase of IgD and low ECG voltage. Bone‐marrow puncture revealed an increase (27%) of plasma cells with some atypicality. Serum immunoelectrophoresis clarified IgD λ‐type paraproteinemia and λ‐type Bence Jones proteinemia.Histologically, edema with an increase in GAG was conspicuous in the circumscribed areas of the middle dermis. Amyloid deposition was clarified in the walls of arterioles in the deep dermis.
Primary Malignant Melanoma of the Penis
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Nishihara Kiyoshi , Imai Toshiya , Kanamori Sachio 
Published: April 2015

AbstractA 50‐year‐old man had a primary malignant melanoma of the penis associated with granuloma telangiectaticum in the pigmented lesion. The patient had an operation for phimosis at the age of 20 and after the operation, he noticed a pigmented lesion at the operation site.We examined the literature and found 16 cases of primary malignant melanoma of the penis in Japan.
Pityriasis Lichenoides‐like Eruption Occurring during Therapy for Myelogenous Leukemia
Authors: Isoda Midori 
Published: April 2015

AbstractA 61‐year‐old Japanese man with chronic myelogenous leukemia developed pityriasis lichenoides‐like eruptions during chemotherapy. Histopathological features were also consistent with the disease.The eruption in this case may have been an allergic reaction arising in a depressed immunity induced by chemotherapy.
Seborrheic Keratosis with Focal Acantholysis
Authors: Maeda Manabu , Mori Shunji 
Published: April 2015

AbstractA case of a 70‐year‐old man with several dome‐shaped tumors with acantholysis was reported. The histopathological findings of these black‐brownish colored tumors on the back were compatible with seborrheic keratosis, consisting of basaloid and squamoid cells.Although three cases reported by Tagami et al. (1978) and Uchiyama et al. (1986) showed intraepidermal epithelioma‐like tumor nests in the acanthotic lesions, our case was thought to correspond to another variant of seborrheic keratosis with acantholysis.
Distribution of an Estrogen Receptor‐related Protein (P29) in Normal Skin and in Cultured Human Keratinocytes
Authors: Viac Jacqueline , Su Hong , Réano Alain , Kanitakis Jean , Chardonnet Yvette , Thivolet Jean 
Published: April 2015

AbstractA monoclonal antibody, ERD5, which recognizes a 29Kd phosphoprotein associated with human estrogen receptor of myometrium was used to study the expression of this protein in normal skin and in cultured human keratinocytes. By indirect immunofluorescence, both in vivo and in vitro keratinocytes showed a variable cytoplasmic staining which increased with cell differentiation. SDS gel electrophoresis of soluble extracts of cultured keratinocytes and normal epidermis showed that P29 was a minor protein. Immunoblot analysis demonstrated that ERD5 strongly reacted only with a 29Kd polypeptide band without any cross‐reactivity.These data suggest that keratinocytes might be estrogen sensitive like other cells in which P29 has already been located. The exact role of this protein in the keratinocyte differentiation process and its relationship with estrogen receptors remain to be elucidated.
IL‐1 and PGE2 Productions by the Regional Lymph Node Cells from DNFB‐sensitized Mice
Authors: Natsuaki Masaru , Higasa Minoru , Sagami Seiichiro , Shinka Sohei 
Published: April 2015

AbstractInterleukin 1 (IL‐1) and Prostaglandin E2 (PGE2) are mainly produced by macrophage/monocytes. In this experiment, we investigated the immunological role of macrophage/monocytes in the lymph nodes (LNs) by measuring IL‐1 and PGE2 productions of regional LN cells from dinitrofluorobenzene (DNFB)‐sensitized and ‐tolerant mice. LN cells from sensitized mice produced IL‐1 in remarkable amounts after in vitro antigen stimulation. On the other hand, the LN cells from tolerant mice failed to produce IL‐1 or PGE2. These data indicate that, by modifying cytokine production, macrophage/monocytes in the regional LNs play a key role in the pathomechanism of contact hypersensitivity.
Penis Tuberculid (Papulonecrotic Tuberculid of the Glans Penis): Treatment with a Combination of Rifampicin and an Extract from Tubercle Bacilli (T.B. Vaccine)
Authors: Nakamura Shin‐ichi , Aoki Masako , Nakayama Keiji , Kanamori Sachio , Onda Shutaro 
Published: April 2015

AbstractA case of penis tuberculid (papulonecrotic tuberculid of the glans penis) and its treatment with combination of oral rifampicin (RFP) and injection of an extract from tubercle bacilli (T.B. vaccine) were reported. We also examined the literature and discovered 37 cases of this disease in Japan in the last 10 years (between 1978 and 1987).
Hematoxylin Stainable Epidermal Protein of the Newborn Rat
Authors: Takahashi Masae , Tezuka Tadashi 
Published: April 2015

AbstractBased on the activity of transglutaminase, the change of antigenicity in situ of hematoxylin stainable protein (HSP) purified from 3‐day‐old rat epidermis and located on the cell membrane region of the stratum corneum was investigated by indirect immunofluorescent technique using a polyclonal antibody against hematoxylin stainable protein. Three different techniques were employed: (i) the skin section was incubated with a commercial guinea pig liver transglutaminase (GLT), (ii) the skin section was incubated with an epidermal extract of Tris‐HCl (EX), and (iii) the skin section was incubated with an epidermal extract of 1% Triton X‐100 (EXT). The sections incubated in Tris‐HCl or Triton X‐100 were used as controls. Each incubation was done both in the presence and absence of Ca2+ ions. After these incubations, an indirect immunofluorescent technique using a polyclonal antibody was performed. In the presence of Ca2+ ions, the specific fluorescence of the cell membrane region of the entire stratum corneum cells disappeared after the preincubation with GLT. That of the lower one third of the stratum corneum disappeared after the incubation with EXT. In contrast, in the absence of Ca2+ ions, no preincubation with GLT, EX, or EXT showed any disappearance of the fluorescence anywhere in the stratum corneum. There was also no disappearance of the fluorescence in the control sections. These findings suggest that the antigenicity of HSP in situ could be lost by the activity of transglutaminase.