bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stress Hyperglycemia ratio in the prediction of 1-year outcomes in patients with acute myocardial infarction: A retrospective large sample cohort study
Authors: Yan Ning , Wu Peng , Zhang Zhengjun , Wang Mohan , Ma Ali , Chen Dapeng , Jia Shaobin , Ma Xueping , Li Xiaocheng , Ma Juan 
Published: August 2024

Abstract

Background Stress hyperglycemia ratio (SHR), an index of relative stress hyperglycemia, is linked to the poor clinical outcomes in patients with coronary artery disease (CAD). Nonetheless, the exploration of SHR's relationship with the prognosis of Acute Myocardial Infarction (AMI) remains scant. Therefore, this study aims to elucidate the connection between SHR and the 1-year clinical outcomes following AMI through a large cohort design analysis. Methods In this retrospective study 4012 patients who were diagnosed with AMI were consecutively enrolled from General Hospital of Ningxia Medical University(2016–2019). These patients were stratified into three distinct groups according to the tertiles of the SHR: Group T1 (SHR < 0.90, n = 1337), Group T2 (0.90 ≤ SHR < 1.11, n = 1337), and Group T3 (SHR ≥ 1.11, n = 1338). All patients were clinically followed for 1-years to collect major adverse cardiovascular events (MACEs), which included all-cause death, nonfatal myocardial infarction (MI), rehospitalization for angina, rehospitalization for heart failure (RHF) and stroke. After controlling for different confounding factors, cox regression models and restricted quadratic splines were used to investigate the relationship between SHR and 1-years clinical outcomes. Results During a 1-year follow-up, a total of 229 all-cause mortalities were record, resulting in an all-cause mortality rate of 5.71% (n = 229). Additionally, 861 MACEs were recorded, yielding a MACE rate of 21.46%. After adjusting for covariates, SHR was found to be significantly associated with 1-year MACE [hazard ratio (HR) = 2.18; 95% confidence interval (CI) = 1.64–2.89; p < 0.001] and all-cause mortality (HR = 3.11; 95% CI = 1.77–5.46; P < 0.001) in patients with AMI, and the T3 group exhibited a higher risk of 1-year MACE (HR = 1.67; 95% CI = 1.34–2.09; p < 0.001) and all-cause mortality (HR = 1.67; 95% CI = 1.02–2.73; p = 0.042) compared with T1 group. A U-shaped association was observed between SHR and 1-year MACE as well as all-cause mortality, with inflection points of 0.87 for poor prognosis in both outcomes. Conclusion SHR is significantly and positively associated with one-year clinical outcomes in patients with AMI. Furthermore, there is a specific non-linear association between SHR and MACE and all-cause mortality (both inflection point 0.87). Interventions aimed at reducing SHR levels below 0.87 through medication management have the potential to significantly improve outcomes.
HDAC inhibitor SAHA resolved intermediate filament bundling and defective autophagy caused by the lack of sacsin in ARSACS.
Authors: Paré Alexandre M , Larochelle Nancy , Dvorak Ryan A. , Minotti Sandra , Atkinson Caitlin S. , Cheng-Boivin Zacharie , Gentil Benoit J. 
Published: August 2024

Abstract

Autosomal recessive spastic ataxia of the Charlevoix-Saguenay (ARSACS) is a progressive childhood-onset neurological disease caused by loss-of-function mutations in the SACS gene. This condition is characterized by cerebellar ataxia, peripheral neuropathy, and spastic plasticity. ARSACS patient-derived fibroblasts and mouse models lacking sacsin exhibit altered mitochondrial networks, intermediate filament bundling, dysregulated autophagic flux, and aberrant protein/organelle localization. Sacsin, a large multidomain protein, plays a critical role in chaperoning and protein homeostasis, particularly in neurons. Dysregulation of autophagy in ARSACS is evidenced by increased LC3 levels and defective lysosome-autophagosome fusion in the absence of sacsin. Recent omics approaches suggest sacsin’s involvement in cytoskeleton regulation, intracellular transport, calcium homeostasis, synaptic organization, and mitochondrial maintenance. This study aimed to explore the effects of histone deacetylase (HDAC) inhibitors on neurofilament (NF) protein acetylation and autophagic flux in ARSACS. NF proteins from Sacs-/- mice showed no significant acetylation differences compared to controls, though HDAC inhibitor SAHA resolved NF bundling. Purification and analysis of NF proteins confirmed high purity and minor acetylation differences at specific lysines (K214). Treatment with SAHA reduced NF bundling in Sacs-/- motor neurons and promoted lysosomal recruitment to autophagosomes in ARSACS patient-derived fibroblasts, indicating restored autophagic flux. These findings highlight the potential of HDAC inhibitors in resolving cytoskeletal abnormalities and improving autophagic processes in ARSACS, offering promising therapeutic avenues for this debilitating disorder. Further research is needed to elucidate the precise mechanisms underlying these effects and their implications for NF homeostasis and autophagy regulation in ARSACS.
Gucy1α1 specifically marks kidney, heart, lung and liver fibroblasts
Authors: Rudman-Melnick Valeria , Vanhoutte Davy , Stowers Kaitlynn , Sargent Michelle , Adam Mike , Ma Qing , Perl Anne Karina T. , Miethke Alexander G. , Burg Ashley , Shi Tiffany , Hildeman David A. , Woodle E. Steve S. , Kofron J. Matthew , Devarajan Prasad 
Published: August 2024

Abstract

Fibrosis is a common outcome of numerous pathologies, including chronic kidney disease (CKD), a progressive renal function deterioration. Current approaches to target activated fibroblasts, key effector contributors to fibrotic tissue remodeling, lack specificity. Here, we report Gucy1α1 as a specific kidney fibroblast marker. Gucy1α1 levels significantly increased over the course of two clinically relevant murine CKD models and directly correlated with established fibrosis markers. Immunofluorescent (IF) imaging showed that Gucy1α1 comprehensively labelled cortical and medullary quiescent and activated fibroblasts in the control kidney and throughout injury progression, respectively. Unlike traditionally used markers platelet derived growth factor receptor beta (Pdgfrβ) and vimentin (Vim), Gucy1α1 did not overlap with off-target populations such as podocytes. Notably, Gucy1α1 labelled kidney fibroblasts in both male and female mice. Furthermore, we observed elevated GUCY1α1 expression in the human fibrotic kidney and lung. Studies in the murine models of cardiac and liver fibrosis revealed Gucy1α1 elevation in activated Pdgfrβ-, Vim- and alpha smooth muscle actin (αSma)-expressing fibroblasts paralleling injury progression and resolution. Overall, we demonstrate Gucy1α1 as an exclusive fibroblast marker in both sexes. Due to its multiorgan translational potential, GUCY1α1 might provide a novel promising strategy to specifically target and mechanistically examine fibroblasts.
The Predictive Significance of Serum C-Reactive Protein in Hepatocellular Carcinoma with Microvascular Invasion
Authors: Yang Chaohao , Liang Zhiwei , Zhao Longshuan , Li Renfeng , Ma Pengfei 
Published: August 2024

Abstract

Background
Microvascular invasion (MVI) diagnosis relies on postoperative pathological examinations, underscoring the urgent need for a novel diagnostic method. C-Reactive Protein (CRP), has shown significant relevance to hepatocellular carcinoma (HCC) prognosis. This study aims to investigate the potential relationship between CRP levels and the presence of microvascular invasion in hepatocellular carcinoma.
Methods
Patients were categorized into MVI-positive and MVI-negative groups for analysis. Serum CRP levels were compared between the two groups. And then use LASSO regression to screen variables and build a nomogram.
Results
CRP levels showed significant differences between the MVI-positive and MVI-negative groups. Multivariable logistic regression analysis identified CRP (OR = 4.85, P < 0.001), lnAFP (OR = 3.11, P < 0.001), WBC count (OR = 2.73, P = 0.003), and tumor diameter (OR = 2.38, P = 0.01) as independent predictors of MVI. A nomogram based on these variables showed good predictive performance in both the training and validation cohorts with dual validation.
Conclusion
The clinical prediction nomogram model, which includes serum CRP levels, WBC count, tumor diameter, and serum AFP levels, showed good performance in predicting MVI in both the training and validation cohorts.
Differential Contributions of Working Memory Components and Visual Attention to Young Children’s Varieties of Basic Number Processing
Authors: Wang Yuhan , Yang Zihan , Yu Xiao , Qi Yue , Yang Xiujie 
Published: August 2024

Abstract

Previous research has found that domain-general cognitive abilities, especially working memory and visual selective attention, play crucial roles in primary children’s mathematical performance, while little is known about their roles in basic number processing in kindergarten children at earlier years. The current study investigated whether working memory components and visual selective attention would make significant contributions to children’s basic number processing. A total of 110 Chinese children (M ± SD = 6.28 ± 0.41 years old) were examined with the phonological loop, the visuospatial sketchpad, the central executive, visual selective attention and three number processing tasks (i.e., numerosity estimation, number line estimation and numerical magnitude comparison tasks). Results revealed that the phonological loop accounted for unique variance in children’s performance on numerosity estimation, number line estimation, and numerical magnitude comparison. Both the visuospatial sketchpad and the central executive significantly contributed to numerical magnitude comparison, whereas visual selective attention explained unique variance in children’s performance of numerosity estimation and number line estimation. Our findings suggest that three components of working memory and visual selective attention have differentiated associations with varied basic number processing skills.
Evaluation of the Positional Reproducibility of Sedation Versus Non-sedation State in Pediatric Radiotherapy : A Retrospective Study
Authors: lei zhanquan , SHI Yuequan , LIU Yiqun , GAO Bo , SHAO Kongfeng , LIN Xijin , WU Lizhen , YAO Zhaojie 
Published: August 2024

Abstract

Objective: To assess the positional reproducibility of sedated and non-sedated pediatric tumor patients during radiotherapy through a retrospective analysis of cone-beam computed tomography (CBCT) and planned computed tomography (CT) scan data.
Methods: The positional reproducibility of 29 pediatric tumor patients receiving radiotherapy in sedated and non-sedated states was retrospectively compared. The first CBCT images obtained during CT-based treatment planning were analyzed. The analysis encompassed six-dimensional positional changes, including vertical (Vrt), longitudinal (Lng), lateral (Lat), rotational (Rtn), pitch, and roll directions. Kolmogorov-Smirnov Z nonparametric rank-sum testing was employed to evaluate the positional deviations, considering absolute values regardless of directionality. Data were further stratified based on different fixation methods used during treatment.
Results: Sedated patients exhibited significantly smaller positional deviations in Vrt, Lng, Lat, and Rtn directions in the body membrane group compared with their non-sedated counterparts (P<0.05). Similarly, sedated patients demonstrated reduced positional deviations in Vrt, Lng, Lat, Rtn, and Roll directions in the head and neck group compared with non-sedated patients (P<0.05). Meanwhile, compared with vacuum bag plus body membrane fixation, the head and shoulder film fixation technique proved superior in terms of positional reproducibility during sedated treatment, specifically in Lng, Lat, and Pitch directions (P<0.05). Similarly, compared with the alternative fixation method, the head and shoulder film fixation method showed better positional deviations in Vrt, Lng, Lat, and Rtn directions in non-sedated patients (P<0.05). Conclusion: While sedated radiotherapy may offer advantages in terms of positional reproducibility, the present study underscores the importance of considering non-sedated radiotherapy as a viable option for pediatric tumor patients. Non-sedated treatment not only provides effective tumor control but also mitigates the psychological trauma and long-term side effects associated with repeated sedative drug use. Future studies should further explore the optimal sedation and fixation strategies for pediatric radiotherapy.
Update on the distribution of Anopheles maculipennis s.l. members in the Republic of Moldova with the first record of An. daciae
Authors: Șuleșco Tatiana , Sauer Felix Gregor , Lühken Renke 
Published: August 2024

Abstract

Background: Anopheles maculipennis complex members are important vectors of human malaria and potentially also for other pathogens, but there is a substantial gap in the current knowledge on their distribution, particularly in Eastern Europe. Therefore, the aim of the study was to update the distribution data for the individual members of An. maculipennis complex in the Republic of Moldova.
Methods: New data were collected between 2013 and 2019 using CDC traps and manual aspirators in the Republic of Moldova. Randomly selected 297 An. maculipennis s.l. females from 49 sampling sites were subjected to the molecular species identification targeting the ITS2 gene. These data were combined with previously collected data in the 21st century and historical data before the 1950s from the literature to present a comprehensive overview on the distribution for the members of An. maculipennis complex.
Results: The most prevalent species was An. maculipennis s.s. (number of specimens = 115, 38.9%), followed by An. daciae (72, 24.3%), An. atroparvus (56, 18.6%) and An. messeae (54, 18.2%). Anopheles daciae was firstly reported for the country, while An. melanoon was confirmed by sequencing of PCR products of the specimens collected in 2012 and 2013.
Conclusion: Five members of the An. maculipennis complex are present in the Republic of Moldova: An. atroparvus, An. maculipennis s.s., An. messeae, An. melanoon, and An. daciae. Anopheles labranchiae and An. sacharovi were not detected.
Family economic disparities and the onset of depressive symptoms among middle-aged and elderly residents in urban and rural areas: A cross-sectional study based on CHARLS
Authors: Man Tingfai , Zhao Yongze , Mai Huaxin , Song Xinan , Wang Bohan , SMITH Robert David , Yuan Jia , Yao Lan , Bian Ying 
Published: August 2024

Abstract

Background: Comparisons in family economic differences and the risk of depression among middle-aged and older individuals in China have not been well investigated.
Methods: This cross-sectional study included 10,884 participants aged 45 or older from the fifth wave of the China Health and Retirement Longitudinal Study (CHARLS) in 2020, with 73.56% aged 45-64 and 26.44% aged ≥65. We measured depressive symptoms using the 10-item Centre for Epidemiologic Studies Depression Scale (CES-D-10) with a cut-off score ≥ 10. Family economic status was estimated using family consumption per capita monthly. Log-binomial models with relative risks (RR) and 95% confidence intervals were used to estimate the relationship between family economic situation and depressive symptoms. Two-way interaction analyses provide insights into the associations between levels of consumption expenditure per capita with covariates and depression in both urban and rural households.
Results: The prevalence of depression was higher in rural areas (42.74%) compared to urban areas (35.36%, P < 0.001). Significant differences were found in various factors between urban and rural areas, including monthly family consumption per capita, marital status, education level, chronic disease, age, self-reported health, satisfaction with children, social activities, and cigarette smoking (all P < 0.05). High household consumption per capita (Top 20%) was associated with depressive symptoms in both urban (aRR = 1.295, 95% CI: 1.091-1.538) and rural areas (aRR = 1.226, 95% CI: 1.127-1.335). Having a chronic disease increased the risk of depression in both urban and rural areas regardless of household consumption per capita. In urban areas, marital status and education level influenced the risk of depression, while in rural areas, health status, child satisfaction, and social activity were influential factors.
Conclusions: Association between excessive consumption and depression in urban and rural areas. Healthcare and relevant support organizations in China should emphasize the importance of family economic status in depression among middle-aged and older adults in both urban and rural areas, and offer treatment and preventative strategies to all families.
Rab27a facilities dendritic cell immune tolerogenic capacity
Authors: Bin Dong-Hua , peng shuwang , Wang Zhuo-Ya , Liu Qiao , Wu Yang-Peng , Wu Yang-Dong , Peng Ke-Ping , Tan Sheng-Lan , Yang Ping-Chang , Tian Guixiang 
Published: August 2024

Abstract

Background Dendritic cells (DC) are known to play a crucial role in inducing immune tolerance. The major cytokine that DCs use to induce immune tolerance is IL-10. Rab27a, a small G protein, has been reported to play a significant role in the cellular output process. The aim of this study is to comprehend how Rab27a regulates the secretion of IL-10 by dendritic cells (DCs).Methods DCs were purified from the intestinal tissues using flow cytometry cell sorting. The enzyme-linked immunosorbent assay was used to quantify cytokines in culture supernatant.Results Our observation was that patients with food allergy (FA) had a lower level of Rab27a activation in peripheral DCs than normal controls (NC). The immune tolerogenic ability of FA DCs was defective compared to NC DCs. The data of Rab27a activation levels and the immune tolerogenic capacity of DC were found to have a positive correlation. The activation of Rab27a in DCs was regulated by MADD in a significant way. Rab27a that was activated created a complex with IL-10, which was responsible for transporting IL-10 out of DCs. MADD and Rab27a activation were found to be lower in DCs collected from patients with FA. Re-establishment of MADD expression could restore the immune tolerogenic capacity of FA DCs.
A pan-genomic analysis based multi-epitope vaccine development by targeting Stenotrophomonas maltophilia using reverse vaccinology method: An in-silico approach
Authors: Shovon Md. Hasan Jafre , Imtiaz Md. , Biswas Partha , Tareq Md. Mohaimenul Islam , Zilani Md Nazmul Hasan , Hasan Md. Nazmul 
Published: August 2024

Abstract

Antibiotic resistance in bacteria leads to high mortality rates and healthcare costs, a significant concern for public health. A colonizer of the human respiratory system, Stenotrophomonas maltophilia is frequently associated with hospital-acquired infections in individuals with cystic fibrosis, cancer, and other chronic illnesses. It is a multidrug-resistant pathogen without an approved vaccine. A multi-epitope vaccine against S. maltophilia has been developed using genomic analysis, reverse vaccination, and immunoinformatic methods. By analyzing 81 complete genomes of S. maltophilia strains, it was estimated that there are 1945 core proteins, with 191 of them expected to be virulent. Out of the virulent proteins, three were selected for epitope prediction. Seven epitopes each from CTL, HTL, and B cell were chosen for vaccine development. The vaccine was refined and validated, showing highly antigenic and desirable physicochemical features. Molecular docking assessments revealed stable binding with TLR-4. Molecular dynamic simulation demonstrated stable dynamics with minor alterations. The immune simulation showed similar immune reactions to real life. These results suggest that the vaccine has promising immune receptor binding capabilities and can induce potent immune responses. Further evidence from studies in mice models is needed to confirm immune protection against S. maltophilia.
The effects of maternal health literacy, family functioning and self-efficacy on antepartum depression in pregnant women in China: A moderated mediation model
Authors: Zhang Ling , Ren Li , Li Hongyu , Qiu Haiyan , Yang Hong , Shi Xiaolan , Rong Honghui , Zheng Chuanfen , Li Fengju , Luo Ting , Lu Lu , Lei Enyu , Yi Dali , Chen Ji-an 
Published: August 2024

Abstract

Background
Antepartum depression (AD) is associated with a greater risk of adverse outcomes for both mothers and fetuses. Maternal health literacy (MHL), family functioning (FF), and self-efficacy (SE) are important factors affecting antepartum depression (AD) in pregnant women. Therefore, this study aimed to explore the associations between these factors and whether MHL and SE mediate the relationship between FF and AD.
Methods
This cross-sectional study included 1392 pregnant women from 6 hospitals in Chongqing, China. Data were collected via structured scales, including the Edinburgh Postpartum Depression Scale, the APGAR Family Care Index Scale, the Maternal Health Literacy Scale and the General Self-Efficacy Scale (GSES). Structural equation modeling(SEM) was used to test the hypothesized relationships among the variables.
Results
In our survey, 31.90% participants had depression symptoms. Correlation analysis revealed that all the variables were significantly correlated with AD (p < 0.01). The model indicated that FF had the strongest correlation with AD (β = -0.276, p < 0.01), followed by MHL (β = -0.248, p < 0.01) and self-efficacy (SE) (β = -0.222, p < 0.01). SE fully mediated the relationship between FF and AD and mediated the relationship between MHL and AD. There was no evidence of a mediating effect of MHL on the relationship between FF and AD.
Conclusions
AD is correlated with MHL, SE and FF. MHL and FF can directly affect AD and mediate AD through SE. Future programs should consider MHL promotion, FF improvement and SE strengthening as breakthrough points when planning educational programs.
The Paradoxical Link between Adiponectin and Atrial Fibrillation in Korean Adults
Authors: Lee Jeong-Gil , Yang Jung-Ho , Kweon Sun-Seog , Lee Young-Hoon , Choi Seong-Woo , Ryu So-Yeon , Nam Hae-Sung , Kim Hye-Yeon , Shin Min-Ho 
Published: August 2024

Abstract

Objectives: The relationship between adiponectin, a protein produced by fat cells with cardioprotective properties, and atrial fibrillation (AF) has been inconsistent in previous studies. Adiponectin levels and AF risk have been positively associated in some studies, while others have not. To clarify this association, we aimed to investigate the association between adiponectin and AF in Korean adults.
Methods: The study included 2,439 participants over 50 years of age from the Dong-gu study. Adiponectin levels were measured in the participants' blood and divided into quartiles. AF was defined with self-report or an electrocardiogram. The association between adiponectin and AF was performed by multivariate Poisson regression analysis after adjusting for covariates.
Results: The overall prevalence of AF in the study population was 2.1%. Age and sex-adjusted prevalence of AF among the different adiponectin quartiles were: 1.0% for the first quartile, 1.4% for the second quartile, 2.3% for the third quartile, and 4.3% for the fourth quartile. The prevalence rate ratio (PRR) of AF increased compared to the first quartile. The second quartile had a PRR of 1.53 (95% CI, 0.62-3.73), the third quartile had a PRR of 2.68 (95% CI, 1.06-6.80), and the fourth quartile had the highest PRR of 5.02 (95% CI, 2.14-11.76).
Conclusion: Our cross-sectional study found a paradoxical association between high levels of serum adiponectin and an increased risk of prevalent AF. Further research is necessary to establish whether the link between adiponectin and AF is causal and to uncover the underlying biological mechanisms of this relationship.