bluuebunny/crossref_metadata_2025_split huggingface dataset:
miR‐495 Enhances the Sensitivity of Non‐Small Cell Lung Cancer Cells to Platinum by Modulation of Copper‐Transporting P‐type Adenosine Triphosphatase A (ATP7A)
Authors: Song Liqiang , Li Yan , Li Weina , Wu Shouzhen , Li Zhikui 
Published: September 2013

ABSTRACTCopper‐transporting P‐type adenosine triphosphatase A (ATP7A) is associated with platinum drug resistance in non‐small cell lung cancer (NSCLC). microRNAs (miRNAs) are a class of small non‐coding RNA molecules that regulate gene expression at post‐transcriptional level. In this study, the aim is to explore which miRNAs might participate in the platinum resistance by targeting ATP7A in NSCLC. Using real‐time PCR‐based miRNA expression profiling and bioinformatics, we selected miR‐495 as a candidate miRNA. EGFP reporter assay, real‐time PCR, and Western blot validated that ATP7A was a direct target for miR‐495. The drug sensitivity assay indicated that miR‐495 enhanced the cell response to cisplatin (CDDP) in NSCLC cells, while inhibition of miR‐495 led to the opposite effects. Importantly, either overexpression or knockdown of ATP7A could override the effect of miR‐495 on chemosensitivity. We also demonstrated that miR‐495 increased the intracellular CDDP accumulation and overexpression of ATP7A can reduce the increased drug concentration induced by miR‐495. Finally, we discovered that there was a converse relationship between miR‐495 and ATP7A levels in NSCLC tissues sensitive or resistant to CDDP. In conclusion, our data demonstrate that miR‐495 regulates the multi‐drug resistance by modulation of ATP7A expression in NSCLC and suggest that miR‐495 may serve as a potential biomarker for the treatment of multi‐drug resistant NSCLC patients with high ATP7A levels. J. Cell. Biochem. 115: 1234–1242, 2014. © 2013 Wiley Periodicals, Inc.
Advances and challenges in gene‐based approaches for osteoarthritis
Authors: Madry Henning , Cucchiarini Magali 
Published: September 2013

AbstractOsteoarthritis (OA), a paramount cause of physical disability for which there is no definitive cure, is mainly characterized by the gradual loss of the articular cartilage. Current nonsurgical and reconstructive surgical therapies have not met success in reversing the OA phenotype so far. Gene transfer approaches allow for a long‐term and site‐specific presence of a therapeutic agent to re‐equilibrate the metabolic balance in OA cartilage and may consequently be suited to treat this slow and irreversible disorder. The distinct stages of OA need to be respected in individual gene therapy strategies. In this context, molecular therapy appears to be most effective for early OA. A critical step forward has been made by directly transferring candidate sequences into human articular chondrocytes embedded within their native extracellular matrix via recombinant adeno‐associated viral vectors. Although extensive studies in vitro attest to a growing interest in this approach, data from animal models of OA are sparse. A phase I dose‐escalating trial was recently performed in patients with advanced knee OA to examine the safety and activity of chondrocytes modified to produce the transforming growth factor β1 via intra‐articular injection, showing a dose‐dependent trend toward efficacy. Proof‐of‐concept studies in patients prior to undergoing total knee replacement may be privileged in the future to identify the best mode of translating this approach to clinical application, followed by randomized controlled trials. Copyright © 2013 John Wiley & Sons, Ltd.
Human JC polyomavirus in normal colorectal mucosa, hyperplastic polyps, sporadic adenomas, and adenocarcinomas in Portugal
Authors: Coelho Tatiana Rasteiro , Gaspar Rita , Figueiredo Pedro , Mendonça Cristina , Lazo Pedro A. , Almeida Luís 
Published: September 2013

AbstractJohn Cunningham virus (JCV) infects chronically human populations worldwide and probably might confer a higher risk for colorectal cancer (CRC). The prevalence of JCV DNA has been determined in normal colon mucosa and compared it with different degrees of colorectal lesions, as well as viral presence in the urine of the individuals in the study. JCV DNA was detected by a nested‐PCR approach targeting the JCV small‐t antigen in 100 healthy controls, and 100 patients undergoing biopsy for diagnosis of colorectal disorders. JCV DNA was detected in 40% of normal mucosa from controls and patients. JCV DNA presence in urine was also similar in controls and patients (37–41% range). JCV DNA detection in normal mucosa and urine reflects the infected population in Portugal. However, in cases with colorectal tumor lesions, JCV DNA was detected in 90% cases, independently of histological type or grade, and this increase was significantly higher with respect to its normal surrounding mucosa. This higher detection of JCV DNA in tumor lesions with respect to its own normal mucosa suggested that a selection for virus containing cells has occurred at some early stage in tumor initiation or progression. JCV may have a specific tropism for colon epithelial cells with some inherent predisposition that makes them more prone to oncogenic transformation, with selection of infected cells. Several p53 polymorphisms in intron 2, common to both groups, were more frequently detected in colorectal pathology cases. A novel p53 mutation in the 3′ untranslated region (exon 11) was identified in 10 patients. J. Med. Virol. 85:2119–2127, 2013. © 2013 Wiley Periodicals, Inc.
Important roles played by TGF‐β in hepatitis B infection
Authors: Karimi‐Googheri Masoud , Daneshvar Hamid , Nosratabadi Reza , Zare‐Bidaki Mohammad , Hassanshahi Gholamhossein , Ebrahim Maryam , Arababadi Mohammad Kazemi , Kennedy Derek 
Published: September 2013

AbstractHepatitis B virus (HBV) which includes, fulminant, acute, chronic, asymptomatic, and occult HBV infection is the most prevalent virus that leads to human liver diseases. Chronic, asymptomatic, and occult infection can induce further sever diseases such as hepatocellular carcinoma (HCC) and cirrhosis of the liver. The underlying mechanisms that allow progression of the prolonged forms of the infection and subsequent HCC or cirrhosis of the liver are yet to be clarified. However, many researchers have suggested that immunological and genetic parameters may play important roles in the etiology of hepatitis B. Transforming growth factor beta (TGF‐β) is an important cytokine with dual regulatory functions in the immune system and in the responses against viral infections. However, the pathways and mechanisms controlling these are not fully understood. The crucial roles of TGF‐β in the development of Th17 and T regulatory lymphocytes, the main cell types involved in autoimmunity and destructive immune related diseases, have been documented and this provides insights into TGF‐β function during hepatitis infection and subsequent HCC and cirrhosis of the liver. Recent findings also confirm that TGF‐β directly alters hepatocyte function during hepatitis B, hence, the aim of this review is to address the current data regarding the association and status of TGF‐β with hepatitis B infection and its related disorders including HCC and cirrhosis of the liver. J. Med. Virol. 86:102–108, 2014. © 2013 Wiley Periodicals, Inc.
Revised Chronology of the Sulmona Lacustrine Succession, Central Italy
Authors: GIACCIO BIAGIO , CASTORINA FRANCESCA , NOMADE SEBASTIEN , SCARDIA GIANCARLO , VOLTAGGIO MARIO , SAGNOTTI LEONARDO 
Published: September 2013

ABSTRACTWe present new stratigraphic, palaeomagnetic, 87Sr/86Sr and 40Ar/39Ar data from a lacustrine succession of the Sulmona basin, central Italy, which, according to an early study, included six unconformity‐bounded lacustrine units (from SUL6, oldest, to SUL1, youngest) spanning the interval >600 to 2 ka. The results of the present study, on the one hand confirm some of the previous conclusions, but by contrast reveal that units SUL2 and SUL1, previously attributed to the Holocene, are actually equivalent to the older SUL6 and SUL5 units – here dated to ∼814–>530 ka and ∼530–<457 ka, respectively – and that the U‐series dates previously published for both former SUL2 and SUL1 units yielded abnormally young ages. In light of the present results, a reassessment of the chronology of the Sulmona basin succession and a revision of the tephrostratigraphy of the SUL2/SUL6 and SUL1/SUL5 units is in order. Copyright © 2013 John Wiley & Sons, Ltd.
Combined oxygen and silicon isotope analysis of diatom silica from a deglacial subarctic Pacific record
Authors: MAIER E. , CHAPLIGIN B. , ABELMANN A. , GERSONDE R. , ESPER O. , REN J. , FRIEDRICHSEN H. , MEYER H. , TIEDEMANN R. 
Published: September 2013

ABSTRACTWe present an SiF4 separation line, coupled to a laser fluorination system, which allows for an efficient combined silica δ18O and δ30Si analysis (50 min per sample). The required sample weight of 1.5–2.0 mg allows for high‐resolution isotope studies on biogenic opal. Besides analytical tests, the new instrumentation set‐up was used to analyse two marine diatom fractions (>63 µm, 10–20 µm) with different diatom species compositions extracted from a Bølling/Allerød–Holocene core section [MD01‐2416, North‐West (NW) Pacific] to evaluate the palaeoceanographic significance of the diatom isotopic signals and to address isotopic effects related to contamination and species‐related isotope effects (vital and environmental effects). While δ30Si offsets between the two fractions were not discernible, supporting the absence of species‐related silicon isotope effects, systematic offsets occur between the δ18O records. Although small, these offsets point to species‐related isotope effects, as bias by contamination can be discarded. The new records strengthen the palaeoceanographic history during the last deglaciation in the NW Pacific characterized by a sequence of events with varying surface water structure and biological productivity. With such palaeoceanographic evolution it becomes unlikely that the observed systematic δ18O offsets signal seasonal temperature variability. This calls for reconsideration of vital effects, generally excluded to affect δ18O measurements. Copyright © 2013 John Wiley & Sons, Ltd.
New Lateglacial fauna and early Mesolithic human remains from northern England
Authors: SMITH IAN R. , WILKINSON DAVID M. , O'REGAN HANNAH J. 
Published: September 2013

ABSTRACTFauna from the Lateglacial Interstadial are largely limited to isolated findspots. Here we report on material from the site of Kent's Bank Cavern, Cumbria, dated to the Allerød/Younger Dryas boundary. The identification of elk (Alces alces) and horse (Equus sp.) and the inferred presence of a large canid indicates that fauna of economic utility to humans were in this area at the time. The presence of humans is attested to by records of Federmesser points from two nearby cave sites. We also report on early Mesolithic human remains from Kent's Bank Cavern. This assemblage represents one of very few Lateglacial faunas in northern Britain, and the most northerly early Mesolithic human remains in the British Isles, making Kent's Bank of importance to discussions of both resource distribution and mortuary rituals in these periods. Copyright © 2013 John Wiley & Sons, Ltd.
Prevalence of ventilation blockages in patients affected by attic pathology: A case‐control study
Authors: Marchioni Daniele , Mattioli Francesco , Alicandri‐Ciufelli Matteo , Presutti Livio 
Published: September 2013

Objectives/HypothesisUsing a retrospective video evaluation of exclusive endoscopic middle ear procedures, we analyzed the different anatomical and pathological findings between patients with attic disease or cholesteatoma and patients without attic disease.Study DesignA case‐control study based on a retrospective chart review in a tertiary university referral center.MethodsVideos from endoscopic middle ear procedures carried out between February 2007 and August 2012 were reviewed. We analyzed and compared two groups of patients who underwent surgery in our university hospital: patients with selective attic disease (e.g., non–self‐cleaning attic selective retraction pockets or attic cholesteatoma) were compared with subjects without attic disease (e.g., chronic middle ear disease, congenital cholesteatoma, or secondary cholesteatoma).ResultsIn total, 152 patients were included in the study. Of these, 102 were affected by primary acquired cholesteatoma (84/102) or severe pars flaccida retraction pockets (18/102), whereas 50 cases were controls. A blockage of the isthmus was present in almost all cases (98/102) compared with a lower prevalence in controls (3/50) (P < .0001). Similarly, a complete tensor fold was identified in 99/102 patients compared to controls (9/46; data were not available in four controls) (P = .001).ConclusionsSelective epitympanic dysventilation occurred when a blockage of the isthmus was present and associated with a complete tensor fold, causing ventilatory separation between the epitympanum and mesotympanum. This mechanism could be the basis for a selective decrease in pressure in the attic, with the subsequent development of attic retraction pockets and cholesteatoma even in subjects with a normally functioning eustachian tube.Level of Evidence3b Laryngoscope, 123:2845–2853, 2013
Determination of the Mode of Radical Termination from Pulsed Laser Polymerization Experiments in the Presence of Retardation and Chain Transfer to Agent
Authors: Nikitin Anatoly N. , Hutchinson Robin A. , Hesse Pascal 
Published: September 2013

The pulsed laser polymerization–high termination rate limit–size‐exclusion chromatography (PLP–HTRL–SEC) technique is used to estimate the mode of termination (δ) for n‐butyl methacrylate (n‐BMA) polymerization at 30 °C. It is found that molecular mass distributions measured in these experiments are influenced by an unknown side reaction such as retardation or chain transfer that results in a marked decrease of intensity of the PLP peak. A new approach is developed to evaluate δ, with numerical experiments used to demonstrate the robustness of the methodology in the presence of retardation or chain transfer to agent. Application to the experimental n‐BMA polymerization at 30 °C leads to an estimate for δ of 0.60 ± 0.03.
image
A role of zinc‐finger protein 143 for cancer cell migration and invasion through ZEB1 and E‐cadherin in colon cancer cells
Authors: Paek A Rome , Lee Chang‐Hoon , You Hye Jin 
Published: September 2013

AbstractTo investigate the role of zinc‐finger protein 143 in cancer cells, we stably introduced ZNF143 expression knockdown by infecting colon cancer cells with short hairpin (sh) RNA‐lentiviral particles against ZNF143 (HCT116 sh‐ZNF143). Compared to sh‐control cells, HCT116 sh‐ZNF143 cells showed faster wound healing, increased migration through Transwell chambers, and increased invasion through Matrigel in Transwell chambers. ZNF143 knockdown increased transcriptional expression of ZEB1. Additionally, ZNF143 regulated E‐cadherin transcriptional expression. Small interfering‐RNA‐mediated silencing of ZEB1 expression affected motility in HCT116 sh‐ZNF143 cells. These data suggest that ZNF143 is involved in cellular motility through a ZEB1‐E‐cadherin‐linked pathway in colon cancer cells. © 2013 Wiley Periodicals, Inc.
Does episiotomy protect against injury of the levator ani muscle in normal vaginal delivery?
Authors: Cassadó Jordi , Pessarrodona Antoni , Rodriguez‐Carballeira Monica , Hinojosa Lourdes , Manrique Gemma , Márquez Adriana , Macias Marina 
Published: September 2013

AbstractAimsTo investigate whether episiotomy is associated with avulsion of the levator ani in its pubic insertion after normal vaginal delivery.MethodsThis is an observational study at a tertiary obstetric unit recruited 194 primiparous women who had normal vaginal delivery with or without episiotomy. All women underwent translabial 4D ultrasound scanning after delivery. Tomographic ultrasound imaging was used to diagnose levator avulsion. Lesions were recorded as unilateral or bilateral. The investigators were blinded to all clinical data. The influence of other variables associated with delivery such as maternal age, body mass index, gestational age, birth weight, fetal head circumference, and use of epidural anesthesia was recorded and their relation with avulsion was also studied.ResultsAvulsion was identified in eleven (10.9%) of the 101 women with episiotomy and in fourteen (15.1%) of the 93 women without. The difference was not statistically significant (P = 0.401). Other variables showed no influence on the prevalence of avulsion.ConclusionsEpisiotomy does not appear to be associated with injury to the levator ani muscle in its pubic insertion in normal vaginal delivery. Neurourol. Urodynam. 33:1212–1216, 2014. © 2013 Wiley Periodicals, Inc.
A review of covariate selection for non‐experimental comparative effectiveness research
Authors: Sauer Brian C. , Brookhart M. Alan , Roy Jason , VanderWeele Tyler 
Published: September 2013

ABSTRACTThis paper addresses strategies for selecting variables for adjustment in non‐experimental comparative effectiveness research and uses causal graphs to illustrate the causal network that relates treatment to outcome. Variables in the causal network take on multiple structural forms. Adjustment for a common cause pathway between treatment and outcome can remove confounding, whereas adjustment for other structural types may increase bias. For this reason, variable selection would ideally be based on an understanding of the causal network; however, the true causal network is rarely known. Therefore, we describe more practical variable selection approaches based on background knowledge when the causal structure is only partially known. These approaches include adjustment for all observed pretreatment variables thought to have some connection to the outcome, all known risk factors for the outcome, and all direct causes of the treatment or the outcome. Empirical approaches, such as forward and backward selection and automatic high‐dimensional proxy adjustment, are also discussed. As there is a continuum between knowing and not knowing the causal, structural relations of variables, we recommend addressing variable selection in a practical way that involves a combination of background knowledge and empirical selection and that uses high‐dimensional approaches. This empirical approach can be used to select from a set of a priori variables based on the researcher's knowledge to be included in the final analysis or to identify additional variables for consideration. This more limited use of empirically derived variables may reduce confounding while simultaneously reducing the risk of including variables that may increase bias. Copyright © 2013 John Wiley & Sons, Ltd.
Research on the Size of Defects inside RDX/HMX Crystal and Shock Sensitivity
Authors: Hua Cheng , Zhang Pan‐Jun , Lu Xiao‐Jun , Huang Ming , Dai Bin , Fu Hua 
Published: September 2013

AbstractIntragranular defects inside RDX/HMX were studied by optical microscopy with matching refractive (OMS), sink‐float method (SFM), and micro‐focus CT (μCT) techniques. OMS results revealed the phenomenon that RDX/HMX had more defects and cracks than RS‐RDX/RS‐HMX. μCT results indicated that RDX/HMX had more defects with larger volume than RS‐RDX/RS‐HMX. The gap test showed that critical shock pressure/gap thickness was 6.4 GPa/19.4 mm for PBX based on RDX, while they were 7.5 GPa/17.5 mm and 8.6 GPa/16.2 mm for PBX based on M‐RDX and RS‐RDX, respectively. Meanwhile, an analysis of the relationship between defects inside RDX/HMX crystal and shock sensitivity was made. Finally, the shock pressure response under impact loading was investigated by discrete element method.
Vibrational properties of semiconductor nanowires and nanowire heterostructures: ensembles and single nanowires
Authors: Cros Ana 
Published: September 2013

Abstractmagnified imageRaman spectroscopy is becoming a standard method for the non‐destructive characterization of nanowires and their heterostructures due to its ability to deliver information about size, structure, carrier concentration, local density, strain and orientation. Several phenomena related to vibrations and the anisotropic nature of nanowires have been reported. The so called “Raman antenna effect” leads to an increase of the scattering intensity that depends on nanowire size, orientation and excitation wavelength. Surface related vibrational modes have been proved to be sensitive to nanowire dimensions and their dielectric environment. Interface and confined modes characteristic of nanowire heterostructures have also been reported, together with low frequency modes that involve the vibration of the nanowire as a whole. Resonant inelastic light scattering has demonstrated its capability of providing information about the internal structure of individual nanowires and nanowire ensembles, increasing at the same time the Raman scattering efficiency. Furthermore, the coupling of the phonons with real electronic excitations gives access to band structure characteristics that cannot be accessed by other means. In this review we analyse the most recent advances concerning the vibrational properties of nanowire ensembles and single nanowires, paying special attention to their potential applications. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
High aspect ratio semiconducting nanostructure random networks: highly versatile materials for multiple applications
Authors: Ternon Céline , Serre Pauline , Rey Germain , Holtzinger Claire , Periwal Priyanka , Martin Mickaël , Baron Thierry , Stambouli Valérie , Langlet Michel 
Published: September 2013

Abstractmagnified imageIn this work we demonstrate that, based on a simple and versatile fabrication method, the collective use and integration of high aspect ratio (AR) nanostructures, here Si and ZnO nanowires (NWs), is possible and addresses multiple application fields, including light detection, active materials for bio‐sensing devices, or materials with interesting wettability properties. A suitable implementation of the filtration method enables then to design networks composed of semiconducting NWs with AR in the range of 10–400. Even for the lowest AR, the network is coherent. However, good electrical properties and flexibility are obtained only for AR greater than 50.magnified imageSi nanowire (SiNW) random networks observed by SEM: Large magnification showing the nanowires and low magnification showing the electrical test structure.(© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)