bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perspectives of older adults on co-management of low back pain by doctors of chiropractic and family medicine physicians: a focus group study
Authors: Lyons Kevin J , Salsbury Stacie A , Hondras Maria A , Jones Mark E , Andresen Andrew A , Goertz Christine M 
Published: September 2013

Abstract

Background
While older adults may seek care for low back pain (LBP) from both medical doctors (MDs) and doctors of chiropractic (DCs), co-management between these providers is uncommon. The purposes of this study were to describe the preferences of older adults for LBP co-management by MDs and DCs and to identify their concerns for receiving care under such a treatment model.

Methods
We conducted 10 focus groups with 48 older adults who received LBP care in the past year. Interviews explored participants’ care seeking experiences, co-management preferences, and perceived challenges to successful implementation of a MD-DC co-management model. We analyzed the qualitative data using thematic content analysis.

Results
Older adults considered LBP co-management by MDs and DCs a positive approach as the professions have complementary strengths. Participants wanted providers who worked in a co-management model to talk openly and honestly about LBP, offer clear and consistent recommendations about treatment, and provide individualized care. Facilitators of MD-DC co-management included collegial relationships between providers, arrangements between doctors to support interdisciplinary referral, computer systems that allowed exchange of health information between clinics, and practice settings where providers worked in one location. Perceived barriers to the co-management of LBP included the financial costs associated with receiving care from multiple providers concurrently, duplication of tests or imaging, scheduling and transportation problems, and potential side effects of medication and chiropractic care. A few participants expressed concern that some providers would not support a patient-preferred co-managed care model.

Conclusions
Older adults are interested in receiving LBP treatment co-managed by MDs and DCs. Older adults considered patient-centered communication, collegial interdisciplinary interactions between these providers, and administrative supports such as scheduling systems and health record sharing as key components for successful LBP co-management.
Inactivation of the novel avian influenza A (H7N9) virus under physical conditions or chemical agents treatment
Authors: Zou Shumei , Guo Junfeng , Gao Rongbao , Dong Libo , Zhou Jianfang , Zhang Ye , Dong Jie , Bo Hong , Qin Kun , Shu Yuelong 
Published: September 2013

Abstract

Background
In the spring of 2013, a novel avian-origin influenza A (H7N9) virus in Eastern China emerged causing human infections. Concerns that a new influenza pandemic could occur were raised. The potential effect of chemical agents and physical conditions on inactivation of the novel avian influenza H7N9 virus had not been assessed.

Methods
To determine the inactivation effectiveness of the novel avian influenza A (H7N9) virus under various physical conditions and chemical treatments, two H7N9 viruses A/Anhui/1/2013 and A/Shanghai/1/2013 were treated by varied temperatures, ultraviolet light, varied pHs and different disinfectants. The viruses with107.7 EID50 were exposed to physical conditions (temperature, ultraviolet light and pH) or treated with commercial chemical agents (Sodium Hypochlorite, Virkon®-S, and Ethanol) respectively. After these treatments, the viruses were inoculated in SPF embryonated chicken eggs, the allantoic fluid was collected after 72–96 hours culture at 35°C and tested by haemagglutination assay.

Results
Both of the tested viruses could tolerate conditions under 56°C for 15 minutes or 60°C for 5 minutes, but their infectivity was completely lost under 56°C for 30 minutes, 65°C for 10 minutes, 70°C, 75°C and 100°C for 1 minute. It was also observed that the H7N9 viruses lost their infectivity totally after exposure of ultraviolet light irradiation for 30 minutes or longer time. Additionally, the viruses were completely inactivated at pH less than 2 for 0.5 hour or pH 3 for 24 hours, however, viruses remained infectious under pH treatment of 4–12 for 24 hours. The viruses were totally disinfected when treated with Sodium Hypochlorite, Virkon®-S and Ethanol at recommended concentrations after only 5 minutes.

Conclusions
The novel avian influenza A (H7N9) virus can be inactivated under some physical conditions or with chemical treatments, but they present high tolerance to moderately acidic or higher alkali conditions. The results provided the essential information for public health intervention of novel H7N9 avian influenza outbreak.
Ethnobotanical appraisal and cultural values of medicinally important wild edible vegetables of Lesser Himalayas-Pakistan
Authors: Abbasi Arshad Mehmood , Khan Mir Ajab , Shah Munir H , Shah Mohammad Maroof , Pervez Arshad , Ahmad Mushtaq 
Published: September 2013

Abstract
Background
The association among food and health is momentous as consumers now demand healthy, tasty and natural functional foods. Knowledge of such food is mainly transmitted through the contribution of individuals of households. Throughout the world the traditions of using wild edible plants as food and medicine are at risk of disappearing, hence present appraisal was conducted to explore ethnomedicinal and cultural importance of wild edible vegetables used by the populace of Lesser Himalayas-Pakistan.

Methods
Data was collected through informed consent semi-structured interviews, questionnaires, market survey and focus group conversation with key respondents of the study sites including 45 female, 30 children and 25 males. Cultural significance of each species was calculated based on use report.

Results
A total of 45 wild edible vegetables belonging to 38 genera and 24 families were used for the treatment of various diseases and consumed. Asteraceae and Papilionoideae were found dominating families with (6 spp. each), followed by Amaranthaceae and Polygonaceae. Vegetables were cooked in water (51%) followed by diluted milk (42%) and both in water and diluted milk (7%). Leaves were among highly utilized plant parts (70%) in medicines followed by seeds (10%), roots (6%), latex (4%), bark, bulb, flowers, tubers and rhizomes (2% each). Modes of preparation fall into seven categories like paste (29%), decoction (24%), powder (14%), eaten fresh (12%), extract (10%), cooked vegetable (8%) and juice (4%). Ficus carica was found most cited species with in top ten vegetables followed by Ficus palmata, Bauhinia variegata, Solanum nigrum, Amaranthus viridis, Medicago polymorpha, Chenopodium album, Cichorium intybus, Amaranthus hybridus and Vicia faba.

Conclusions
Patterns of wild edible plant usage depend mainly on socio-economic factors compare to climatic conditions or wealth of flora but during past few decades have harshly eroded due to change in the life style of the inhabitants. Use reports verified common cultural heritage and cultural worth of quoted taxa is analogous. Phytochemical analysis, antioxidant activities, pharmacological applications; skill training in farming and biotechnological techniques to improve the yield are important feature prospective regarding of wild edible vegetables.
Lemon protein disulfide isomerase: cDNA cloning and biochemical characterization
Authors: Chen Yu-Ting , Wen Lisa , Ho Kuo-Chuan , Juang Rong-Huay , Lin Chi-Tsai 
Published: September 2013

Abstract

Background
Protein disulfide isomerases (PDIs), a family of structurally related enzymes, aid in protein folding by catalyzing disulfide bonds formation, breakage, or isomerization in newly synthesized proteins and thus.

Results
A ClPDI cDNA (1828 bp, GenBank accession HM641784) encoding a putative PDI from Citrus limonum was cloned by polymerase chain reaction (PCR). The DNA sequence encodes a protein of 500 amino acids with a calculated molecular mass of 60.5 kDa. The deduced amino acid sequence is conserved among the reported PDIs. A 3-D structural model of the ClPDI has been created based on the known crystal structure of Homo sapiens (PDB ID: 3F8U_A). The enzyme has two putative active sites comprising the redox-active disulfides between residues 60–63 and 405–408 (motif CGHC). To further characterize the ClPDI, the coding region was subcloned into an expression vector pET-20b (+), transformed into E. coli Rosetta (DE3)pLysS, and recombinant protein expressed. The recombinant ClPDI was purified by a nickel Sepharose column. PDI’s activity was assayed based on the ability of the enzyme to isomerize scrambled RNase A (sRNase A) to active enzyme. The K
M
, k
cat and k
cat/K
M values were 8.3 × 10-3 μM, 3.0 × 10-5 min-1, and 3.6 × 10-1 min-1 mM-1. The enzyme was most active at pH 8.

Conclusions
The advantage of this enzyme over the PDI from all other sources is its low K
M. The potential applications of this PDI in health and beauty may worth pursuing.
Comparative analysis of gene transcripts for cell signaling receptors in bone marrow-derived hematopoietic stem/progenitor cell and mesenchymal stromal cell populations
Authors: Anam Khairul , Davis Thomas A 
Published: September 2013

Abstract
Introduction
Knowing the repertoire of cell signaling receptors would provide pivotal insight into the developmental and regenerative capabilities of bone marrow cell (BMC)-derived hematopoietic stem/progenitor cells (HSPCs) and bone marrow mesenchymal stromal cells (BMMSCs).

Methods
Murine HSPCs were enriched from fluorescence-activated cell sorting (FACS)-sorted Lin–c-Kit+Sca-1+ BMCs isolated from the tibia and femoral marrow compartments. Purified BMMSCs (CD73+, CD90+, CD105+, and CD45–, CD34–, CD31–, c-Kit–) with extensive self-renewal potential and multilineage differentiation capacity (into different mesodermal cell lineages including osteocytes, chrondrocytes, adipocytes) were derived from adherent BMC cultures after CD45+ cell depletion. Adherent colony-forming cells were passaged two to three times and FACS analysis was used to assess cell purity and validate cell-specific surface marker phenotype prior to experimentation. Gene transcripts for a number of cell signaling molecules were assessed using a custom quantitative real-time RT-PCR low-density microarray (94 genes; TaqMan® technology).

Results
We identified 16 mRNA transcripts that were specifically expressed in BMC-derived HSPC (including Ptprc, c-Kit, Csf3r, Csf2rb2, Ccr4, Cxcr3 and Tie-1), and 14 transcripts specifically expressed in BMMSCs (including Pdgfra, Ddr2, Ngfr, Mst1r, Fgfr2, Epha3, and Ephb3). We also identified 27 transcripts that were specifically upregulated (≥2-fold expression) in BMMSCs relative to HSPCs (Axl, Bmpr1a, Met, Pdgfrb, Fgfr1, Mertk, Cmkor1, Egfr, Epha7, and Ephb4), and 19 transcripts that were specifically upregulated in HSPCs relative to BMMSCs (Ccr1, Csf1r, Csf2ra, Epor, IL6ra, and IL7r). Eleven transcripts were equally expressed (<2-fold upregulation) in HSPCs and BMMSCs (Flt1, Insr, Kdr, Jak1, Agtrl1, Ccr3, Ednrb, Il3ra, Hoxb4, Tnfrsf1a, and Abcb1b), whilst another seven transcripts (Epha6, Epha8, Musk, Ntrk2, Ros1, Srms, and Tnk1) were not expressed in either cell population.

Conclusions
We demonstrate that besides their unique immunophenotype and functional differences, BMC-derived HSPCs and BMMSCs have different molecular receptor signaling transcript profiles linked to cell survival, growth, cell differentiation status, growth factor/cytokine production and genes involved in cell migration/trafficking/adhesion that may be critical to maintain their pluripotency, plasticity, and stem cell function.
Interrogating Escapism: Rethinking Kenneth Branagh’s Love’s Labour’s Lost
Authors: Severn John R. 
Published: September 2013

In a departure from the widespread critical readings of Kenneth Branagh's cinematic adaptation of Love's Labour's Lost (2000) as a failed attempt to recreate the 1930s Hollywood film musical, this article takes a hermeneutic, rather than evaluative, approach in centralising the fragmented elements of pastiche which destabilise the film's readability as a unified text. Engaging with the manifold criticisms levelled against Branagh's fourth and arguably most radical Shakespearean adaptation, the argument emphasises the film's discontinuities and the often-questioned performance skills of its actors, positing these as strengths which respond directly to the metatheatricality and pastiche of Shakespeare's texts. Through a rejection of monologic readings of the film, the article instead places value on its apparent disjunctions, which create an interrogative text and invite the viewer to engage with the concept of escapism: a concept often obstructed by Branagh's knowing subversions of genre. By placing a play about a failed attempt at escapism in conversation with the Depression-era Hollywood film musical, one of the most escapist of film genres, Branagh's film, the article argues, opens itself up to interpretations of the film itself, Shakespeare's play, the musical genre, and spectator relationships to escapism.
Periodic and Solitary Wave Solutions of Complex Nonlinear Evolution Equations by Using First Integral Method
Authors: Taşcan Filiz , Bekir Ahmet 
Published: September 2013

Abstract
In this paper, we utilize the first integral method with symbolic computation to constructing periodic and solitary wave solutions of complex nonlinear evolution equations. With the aid of Maple, new exact solutions of two nonlinear evolution equations in physics, namely, generalized Zakharov equations (GZ) and higher-order nonlinear Schrodinger equation (HONLS) describing propagation of ultrashort pulses in nonlinear optical fibers are obtained.
Sylvester-tridiagonal Matrix with Alternating Main Diagonal Entries and its Spectra
Authors: Kılıç Emrah 
Published: September 2013

Abstract
Recently some generalizations of Sylvester type tridiagonal matrices have been considered with their spectra. We introduce a new kind generalization of Sylvester type tridiagonal matrix by considering its main diagonal entries. Then we compute its spectra and determinant.
Parallel processing and the human mind: Re-understanding consciousness with James Joyce's Ulysses
Authors: Hogan Patrick Colm 
Published: September 2013

Abstract
Research on neural models for cognition suggests that thought is far from a simply serial process. Nonetheless, there has been relatively little work on which parameters govern just what aspects of thought are parallel and what are serial. Clearly, speech as such is serial. In consequence, interior monologue (understood as subvocalised speech) is serial. Moreover, stream of consciousness – mental experience not confined to subvocalised speech – must be articulated in serial form in a novel. Due to this constraint on representation, it seems that novelists commonly imagine that stream of consciousness itself really is serial. Joyce, however, developed a sense of parallel cognitive processing in the course of Ulysses. Specifically, in the “Wandering Rocks” episode, he explored spatiotemporally parallel events in complex social systems. In the following chapter, “Sirens,” he in effect transferred this treatment of external parallelism to the human mind, systematically developing cognitive parallelism in his representation of Leopold Bloom. This development was perhaps reinforced by ideas of harmony and counterpoint associated with the episode's musical model. Understanding Joyce's exploration of parallel and serial processes in thought is important not only for what it tells us about Ulysses. It is also important for what it contributes to our understanding of cognitive parallelism.
Affect and artifice in cognitive literary theory
Authors: Polvinen Merja 
Published: September 2013

Abstract
This essay suggests that separating emotionally immersed and reflectively rational ways of experiencing fiction on the basis of ontological structures and spatio-temporal metaphors is hampering our understanding of the experience of fiction. Beginning in a rhetorical approach, I argue for a model where engagement with fiction is seen in terms of joint attention. Using joint attention rather than knowledge of ontological realms as a reference point has two distinct benefits: it refocuses attention on literature as a rhetorical mode, and it takes mental action to be a system of parallel processes, thus giving an alternative to the back-and-forth movement between the interior and exterior of imagined worlds. My focus is on Dave Eggers's A Heartbreaking Work of Staggering Genius (2000), a memoir that embeds an autobiographical narrative within self-reflective commentary, and which explicitly calls attention to the issues of emotional sincerity and rational distance.
Preserving Facebook Records: Subscriber Expectations and Behavior
Authors: Blaha Craig 
Published: September 2013

AbstractThe number of social networking services and the number of subscribers to those services have grown at an exponential rate over the last decade. This study examines how long Facebook subscribers expect their records to persist and how these expectations affect subscriber’s use of Facebook. I use an online survey (n = 144) and a focus group (n = 6) to determine Facebook subscribers’ preservation expectations and to what extent these expectations affect the type of information subscribers choose to share. I found that respondents expect records to persist for less than ten years, and that respondents alter their sharing behavior based on their preservation expectations. A better understanding of subscribers’ attitudes toward preservation, and the effect of these attitudes on the information subscribers choose to share, is an important step in developing a workable preservation strategy for these records.
Soluble guanylate cyclase in NO signaling transduction
Authors: Pan Jie , Zhong Fangfang , Tan Xiangshi 
Published: September 2013

AbstractNitric oxide (NO), a signaling molecule in the cardiovascular system, has been receiving increasing attention since Furchgott, Ignarro, and Murad were awarded the Nobel Prize in Physiology and Medicine for the discovery in 1998. Soluble guanylate cyclase (sGC), as an NO receptor, is a key metalloprotein in mediating NO signaling transduction. sGC is activated by NO to catalyze the conversion of guanosine 5′-triphosphate (GTP) to cyclic guanylate monophosphate (cGMP). The dysfunction of NO signaling results in many pathological disorders, including several cardiovascular diseases, such as arterial hypertension, pulmonary hypertension, heart failure and so on. Significant advances in its structure, function, mechanism, and physiological and pathological roles have been made throughout the past 15 years. We herein review the progress of sGC on structural, functional investigations, as well as the proposed activation/deactivation mechanism. The heme-dependent sGC stimulators and heme-independent sGC activators have also been summarized briefly.
Diagnostic value of a ghrelin test for the diagnosis of GH deficiency after subarachnoid hemorrhage
Authors: Blijdorp K , Khajeh L , Ribbers G M , Sneekes E M , Heijenbrok-Kal M H , van den Berg-Emons H J G , van der Lely A J , van Kooten F , Neggers S J C M M 
Published: September 2013

ObjectiveTo determine the diagnostic value of a ghrelin test in the diagnosis of GH deficiency (GHD) shortly after aneurysmal subarachnoid hemorrhage (SAH).DesignProspective single-center observational cohort study.MethodsA ghrelin test was assessed after the acute phase of SAH and a GH-releasing hormone (GHRH)–arginine test 6 months post SAH. Primary outcome was the diagnostic value of a ghrelin test compared with the GHRH–arginine test in the diagnosis of GHD. The secondary outcome was to assess the safety of the ghrelin test, including patients' comfort, adverse events, and idiosyncratic reactions.ResultsForty-three survivors of SAH were included (15 males, 35%, mean age 56.6±11.7). Six out of 43 (14%) SAH survivors were diagnosed with GHD by GHRH–arginine test. In GHD subjects, median GH peak during ghrelin test was significantly lower than that of non-GHD subjects (5.4 vs 16.6,P=0.002). Receiver operating characteristics analysis showed an area under the curve of 0.869. A cutoff limit of a GH peak of 15 μg/l corresponded with a sensitivity of 100% and a false-positive rate of 40%. No adverse events or idiosyncratic reactions were observed in subjects undergoing a ghrelin test, except for one subject who reported flushing shortly after ghrelin infusion.ConclusionOwing to its convenience, validity, and safety, the ghrelin test might be a valuable GH provocative test, especially in the early phase of SAH.
A Practical Approach for Enhancing Model Accuracy Using Pressure and Permeability Function
Authors: Al-Zahrani Tariq , Al-Hoti Saud 
Published: September 2013

Abstract
In a typical reservoir simulation model, varying details of its geological and petrophysical properties need to be captured accurately. There are single-phase regions, where a considerable savings in costs may be realized if large blocks are used, while there are regions, particularly near well-bores, where fine grids are required to adequately capture high and low permeability streaks. The main goal of reservoir characterization is to get a reservoir model that accurately describes and predicts dynamic fluid flow paths and production/injection performances of the wells. This implies a correct description of extreme values of connections of petrophysical properties, mainly permeability and porosity.
The fine scale model can handle most structural and geological complexity with few compromises. Such a model will allow us to quantify uncertainties and also to run risk analysis. This ensures a realistic and consistent model that honors and maintains the latest field information. In case of updating or adjustment to the simulation model, this can be updated in the geological model as well.
Moreover, history matching, by nature, is a very complex inverse problem that can be computationally intensive and practically difficult for very large multimillion cell reservoir models. Therefore, the use of an optimal parameterization and refinement grid is crucial to get fast and valid history matching results.
This paper shows that fine models provide much more accurate results than the up scaled coarse model. A new method will be introduced to enhance history match quality by conditioning a second version of the permeability model to a relation found between errors in pressure and KH from well test data. A comparison of results including core permeability and saturation from both models are presented. The positive impact of this new method on the history match process is discussed in details.