bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lean and Obese Coronary Perivascular Adipose Tissue Impairs Vasodilation via Differential Inhibition of Vascular Smooth Muscle K
+
Channels
Authors: Noblet Jillian N. , Owen Meredith K. , Goodwill Adam G. , Sassoon Daniel J. , Tune Johnathan D. 
Published: April 2015

Objective—
The effects of coronary perivascular adipose tissue (PVAT) on vasomotor tone are influenced by an obese phenotype and are distinct from other adipose tissue depots. The purpose of this investigation was to examine the effects of lean and obese coronary PVAT on end-effector mechanisms of coronary vasodilation and to identify potential factors involved.

Approach and Results—

Hematoxylin and eosin staining revealed similarities in coronary perivascular adipocyte size between lean and obese Ossabaw swine. Isometric tension studies of isolated coronary arteries from Ossabaw swine revealed that factors derived from lean and obese coronary PVAT attenuated vasodilation to adenosine. Lean coronary PVAT inhibited K
Ca
and K
V
7, but not K
ATP
channel-mediated dilation in lean arteries. In the absence of PVAT, vasodilation to K
Ca
and K
V
7 channel activation was impaired in obese arteries relative to lean arteries. Obese PVAT had no effect on K
Ca
or K
V
7 channel-mediated dilation in obese arteries. In contrast, obese PVAT inhibited K
ATP
channel-mediated dilation in both lean and obese arteries. The differential effects of obese versus lean PVAT were not associated with changes in either coronary K
V
7 or K
ATP
channel expression. Incubation with calpastatin attenuated coronary vasodilation to adenosine in lean but not in obese arteries.

Conclusions—

These findings indicate that lean and obese coronary PVAT attenuates vasodilation via inhibitory effects on vascular smooth muscle K
+
channels and that alterations in specific factors such as calpastatin are capable of contributing to the initiation or progression of smooth muscle dysfunction in obesity.
Chest CT at a dose below 0.3 mSv: impact of iterative reconstruction on image quality and lung analysis
Authors: Nakajo Chiaki , Heinzer Susanne , Montandon Stéphane , Dunet Vincent , Bize Pierre , Feldman Andrei , Beigelman-Aubry Catherine 
Published: April 2015

Background In chest computed tomography (CT), iterative reconstruction (IR) algorithms maintain diagnostic image quality (IQ) while significantly reducing the dose. Purpose To evaluate the impact of IR on IQ of chest CT at effective doses below 0.3 mSv. Material and Methods Twenty chest CT scans performed at effective dose below 0.3 mSv (CT1) were reconstructed varying three parameters: filtered back-projection and IR iDose4 algorithms; 512 × 512 and 768 × 768 matrices; and sharp and soft kernels, thus generating eight series per patient. The qualitative evaluation of the IQ was performed by ranking series from 1 to 8 (8 corresponding to the highest rank) which was subsequently compared to quantitative assessment of IQ by using an appropriated merit formula. Intra- and inter-reader IQ ranking reliability was also evaluated using Cohen’s kappa. Analysis of lung findings was finally compared between the best CT1 series and the reference CT (CT0). Results The best series in terms of qualitative and quantitative IQ was obtained using IR, 5122 matrix and soft kernel. The best CT1 series detected nodules greater than 4 mm with an almost perfect match with CT0. Conclusion Chest CT performed at effective doses below 0.3 mSv may be used to confidently diagnose lesions greater than 4 mm using iDose4, soft kernel and 512 × 512 matrix.
Evaluation of uterine peristalsis using cine MRI on the coronal plane in comparison with the sagittal plane
Authors: Shitano Fuki , Kido Aki , Kataoka Masako , Fujimoto Koji , Kiguchi Kayo , Fushimi Yasutaka , Togashi Kaori 
Published: April 2015

Background Uterine peristalsis is supposed to be closely related to the early stages of reproduction. Sperms are preferentially transported from the uterine cervix to the side of the tube with the dominant follicle. However, with respect to magnetic resonance imaging (MRI), uterine peristalsis has only been evaluated at the sagittal plane of cine MRI. Purpose To evaluate and compare uterine peristalsis both on sagittal and coronal planes using cine MRI. Material and Methods Internal ethics committee approval was obtained, and subjects provided informed written consent. Thirty-one women underwent MRI scans in the periovulatory phase of the menstrual cycle. Cine MR images obtained by fast advanced spin echo sequence at 3-T field strength magnet (Toshiba Medical Systems) were visually evaluated by two independent radiologists. The frequency and the direction of peristalsis, and the presence of outer myometrium conduction of signal intensities (OMC), were evaluated. The laterality of the dominant follicle was determined on axial images and compared with the peristaltic direction in fundus. Results The subjects in which peristaltic directions were more clearly recognized were significantly frequent in coronal planes than in sagittal planes ( P < 0.05). There was no significant difference in the peristaltic frequency between the sagittal and the coronal plane. However, the OMC was more recognized in the coronal plane than in the sagittal plane ( P < 0.05). Peristaltic waves conducted toward the possible ovulation side were observed in only three of the 10 subjects. Conclusion OMC of uterine peristalsis was better demonstrated in the coronal plane compared to the sagittal plane.
In vivo delivery of functional Flightless I siRNA using layer-by-layer polymer surface modification
Authors: Martens Penny J , Ly Mai , Adams Damian H , Penzkover Kathryn R , Strudwick Xanthe , Cowin Allison J , Poole-Warren Laura A 
Published: April 2015

Gene silencing using small interfering RNA has been proposed as a therapy for cancer, viral infections and other diseases. This study aimed to investigate whether layer-by-layer polymer surface modification could deliver small interfering RNA to decrease fibrotic processes associated with medical device implantation. Anti-green fluorescent protein labelled small interfering RNA was applied to tissue culture plates and polyurethane using a layer-by-layer technique with small interfering RNA and poly-L-lysine. In vitro studies showed that the level of down-regulation of green fluorescent protein was directly related to the number of coatings applied. This layer-by-layer coating technique was then used to generate Rhodamine-Flii small interfering RNA-coated implants for in vivo studies of small interfering RNA delivery via subcutaneous implantation in mice. After two days, Rh-positive cells were observed on the implants’ surface indicating cellular uptake of the Rhodamine-Flii small interfering RNA. Decreased Flii gene expression was observed in tissue surrounding the Rhodamine-Flii small interfering RNA coated implants for up to seven days post implantation, returning to baseline by day 21. Genes downstream from Flii, including TGF-β1 and TGF-β3, showed significantly altered expression confirming a functional effect of the Rhodamine-Flii small interfering RNA on gene expression. This research demonstrates proof-of-principle that small interfering RNA can be delivered via layer-by-layer coatings on biomaterials and thereby can alter the fibrotic process.
Should Age Limit the Use of Catheter-Directed Thrombolysis
Authors: Al-Nouri Omar , Sinacore James , Halandras Pegge , Hershberger Richard 
Published: April 2015

Objective: To determine whether advanced age should be a contraindication to catheter-directed thrombolysis (CDT) based on hemorrhagic complication rate. Methods: A survey was generated via Survey Monkey and sent out to vascular surgeons who were members of the society of vascular surgery (SVS). Results: Of the responders, 32.7% state they do not have an age limit for tissue plasminogen activator (TPA) infusion, and the remaining 29.2% of the responders use 80 years of age as their limitation. When asked why place limits on age for TPA infusion, 56.6% stated concern for intracranial hemorrhage. Major complications were access site hemorrhage (58.4%) and intracranial hemorrhage (41.6%). Chi-square analysis did not show age as a limiting factor to thrombolysis. Furthermore, when asked in which age-group complications occurred most commonly, 72.4% were less than 80. Conclusion: Among vascular specialist, there seems to be no consensus on age limitations for TPA infusion. Serious complications do not seem to be age related and thus age alone should not be a contraindication for catheter-directed thrombolysis.
An in vivo study of dynamic effects of wrist traction on the radiolunate and capitolunate joints
Authors: Tada Kaoru , Kawashima Hiroki , Horie Kakeru , Sanada Shigeru , Nishimura Seiji , Tsuchiya Hiroyuki 
Published: April 2015

Introduction We described the dynamic effects of traction on the radiolunate and capitolunate joints in a living body to explore the possibility of range of motion exercise of the wrist joint under traction as a new method of exercise. Methods Dynamic radiography was conducted while creating wrist passive movements by using manual traction and machinery traction with a measurement device. Subjects were 20 healthy men. The change in the percentage of contribution of the radiolunate and capitolunate joints with or without traction was calculated. Results Manual traction: During palmar flexion, the percentage of contribution of the radiolunate angle was increased from 39.4% to 49.9%, and the capitolunate angle was decreased from 60.6% to 50.1% with traction. During dorsiflexion, the radiolunate angle was increased from 60.2% to 70.0%, and the capitolunate angle was decreased from 39.8% to 30.0% with traction. Machinery traction: During palmar flexion, the radiolunate angle was increased from 46.1% to 56.4%, and the capitolunate angle was decreased from 53.9% to 43.6% with traction. During dorsiflexion, the radiolunate angle was increased from 68.7% to 72.0%, and the capitolunate angle was decreased from 31.3% to 28.0% with traction. Conclusions The movement of the radiolunate joint was increased and that of the capitolunate joint decreased with the addition of traction. Range of motion exercise with traction has the potential to be applied to an advanced rehabilitation program targeting the radiolunate joint under specific pathological conditions.
Treatment options for relapsed and refractory multiple myeloma
Authors: Nooka Ajay K. , Kastritis Efstathios , Dimopoulos Meletios A. , Lonial Sagar 
Published: April 2015

Abstract
Over the last few decades, significant improvement in outcomes has been observed for myeloma patients, mainly as a result of the use of currently available approved antimyeloma agents, along with combining autologous stem cell transplantation in the treatment of myeloma. With more targeted agents in development, the treatment of a myeloma patient at relapse has become complicated and, as a consequence, results in vast heterogeneity in treatment patterns. Although a consensus on the timing of initiation of treatment, the choice of agents to be used, and the role of transplant is less clear, we describe an evidence-based approach and the factors to consider upon relapse. We describe additional newer agents and targets that are under development, with the goal of achievement of durable remissions for myeloma patients.
Along-strike variability of thrust fault vergence
Authors: Greenhalgh Scott R. , McBride John H. , Bartley John M. , Keach R. William , Britt Brooks B. , Kowallis Bart J. 
Published: April 2015

The 3D kinematic evolution of thrust systems, in which vergence changes along strike, is poorly understood. This study uses 3D seismic data from Big Piney-LaBarge field, Wyoming, to examine the geometry and kinematics of two faults at the leading edge of the Hogsback thrust sheet, the frontal thrust of the Late Cretaceous Sevier fold-thrust belt. These thrusts lie along strike of each another and share an east-vergent detachment within the Cretaceous Baxter Shale. The two thrusts verge in opposite directions: The southern thrust verges eastward forming a frontal ramp consistent with major thrusts of the Sevier belt, whereas the northern thrust verges westward to form a type 1 triangle zone with the Hogsback thrust. The thrusts have strike lengths of 5 km (3.1 mi) and 8 km (5.0 mi), respectively, and they are separated by a transfer zone of less than 0.5 km (0.3 mi) wide. Strata in the transfer zone appear to be relatively undeformed, but reflections are less coherent here, which suggests small offsets unresolved by the seismic survey. Retrodeformable cross sections and a structure contour map on the Cretaceous Mesaverde Group indicate that shortening varies along strike, with a pronounced minimum at the transfer zone and greater shortening across the northern, west-vergent thrust (610 m [2000 ft]) than across the southern, east-vergent thrust (230 m [755 ft]). Mapping of these thrusts suggests that they propagated laterally toward each other to form a type 1 antithetic fault linkage in the transfer zone. Spatial patterns expressed in seismic attributes in and near the detachment horizon, which include waveform classification and spectral decomposition, suggest that stratigraphic variations may have pinned the detachment, thus localizing the transfer zone. Thickness variations in the thrust sheet also may have influenced the thrust geometry. Our study provides an analog for analysis of similar complex contractional belts around the world.
Structure–Antiamnesic Activity Relationship of Iridoid Glycosides from Gardenia Fruits
Authors: Kwak Jong Hwan , Lee Dong-Ung 
Published: April 2015

Abstract
We investigated the in vitro inhibitory acetylcholinesterase (AChE) activities and in vivo ameliorating effects on scopolamine-induced memory impairment of nine iridoid glycosides, namely, geniposide (1), genipin gentiobioside (2), gardenoside (3), scandoside methyl ester (4), deacetylasperulosidic acid methyl ester (5), 6-O-methyldeacetylasperulosidic acid methyl ester (6), geniposidic acid (7), gardoside (8), and shanzhiside (9), isolated from the fruits of Gardenia jasminoides (edible gardenia). Contrary to preliminary studies, genipin gentiobioside (2) exhibited the greatest anti-AChE activity (60.23 ± 2.12%) and antiamnesic effects (ca. 2-fold enhancement vs. the untreated control) using a passive avoidance task system, which is comparable to tacrine. Structure–activity relationships are suggested, which would be useful for identifying candidate compounds for the treatment of memory dysfunction.
EXAFS Studies of Pd Nanoparticles: Direct Evidence for Unusual Pd–Pd Bond Elongation
Authors: Ohba Tadashi , Kubo Hitoshi , Ohshima Yusuke , Makita Yuichi , Nakamura Noriaki , Uehara Hiromitsu , Takakusagi Satoru , Asakura Kiyotaka 
Published: April 2015

Abstract
Pd nanoparticles exhibit the abnormal behavior of the Pd lattice expanding as the size of the Pd nanoparticles decreases. We measured extended X-ray absorption fine structure of poly(N-vinyl-2-pyrrolidone)-protected Pd nanoparticles and confirmed that the Pd–Pd bond distance in small Pd nanoparticles with a diameter of less than 3 nm was elongated by 2% compared with that in Pd foil. The origin of the unusual bond elongation was discussed.
Antiferromagnetic Transition in a Novel Star-shaped High-spin Fe(III) Tetranuclear Cluster from a Mononuclear Coordination Anion Featuring π-Extended Schiff Base Ligands
Authors: Takahashi Kazuyuki , Kawamukai Kiko , Mochida Tomoyuki , Sakurai Takahiro , Ohta Hitoshi , Yamamoto Takashi , Einaga Yasuaki , Mori Hatsumi , Shimura Yasuyuki , Sakakibara Toshiro , Fujisawa Takumi , Yamaguchi Akira , Sumiyama Akihiko 
Published: April 2015

Abstract
A novel star-shaped high-spin Fe(III) tetranuclear cluster was unexpectedly obtained by the reaction of an [FeIII(phsal)2]− anion with an Fe(II) coordination cation (H2phsal: N-(2-hydroxyphenyl)salicylaldimine). Despite a star-shaped Fe(III) tetranuclear structure similar to reported Fe(III) single molecule magnets, the present compound exhibited a bulk antiferromagnetic transition at 0.6 K due to a magnetic exchange coupling through intercluster π-stacking interactions.
Developing New Systems of Data to Advance a Culture of Health
Authors: Plough Alonzo L. 
Published: April 2015

Introduction: The system dynamics that are driving changes in health and health
care in the United States are not well captured by standard data collection activities.
We need systems of data that can inform policy and program development and reflect the
increasing recognition that all aspects of people’s lives—their work, families, and
communities and not just formal health and health care services—support active and
healthy living.Culture of Health Action Areas: Within the Culture of Health Framework,
there are four action areas that are farther upstream in the sequence of change and
point to additional need for more nuanced approach to data collection: building a shared
value of health; fostering collaboration to improve well-being; creating health more
equitable community environments; and transforming health and health care systems. These
action areas define the Culture of Health framework that we hope will spur research and
strategies across sectors to improve health.Conclusion: The action framework for
advancing a culture of health will guide the Robert Wood Johnson Foundation’s
grant-making and strategic collaborations. The hope is that it will also catalyze
changes in how researchers and organizations collect health related data at all
levels.
Using Pulsed Neutron Decay-Spectrum Data and Multi-inflatable Packer Plugdown Assemblies Improve Oil Production Rates in a Mature CO2 Flood
Authors: Schneider B. D. , Hogan G. P. , Holt W. N. 
Published: April 2015

Abstract
A gravity-stable, vertical C02 miscible injection flood was implemented in the 824 feet thick Wellman Unit Wolfcamp reef reservoir in July 1983. C02 is being injected in the crest of the reservoir to displace the oil vertically downward. The producing wells areconstantly being plugged down out of the "gassed out" interval into the oil bank.
The purpose of this paper is to present the results of the PND®-S through tubing logging tool as a method to determine the C02/oil/water contacts and the use of multiple inflatable packers/sliding sleeve plugdown assemblies to achieve zonal isolation between "gassed out" intervals and producing intervals.
Individuals Poststroke Do Not Perceive Their Spatiotemporal Gait Asymmetries as Abnormal
Authors: Wutzke Clinton J. , Faldowski Richard A. , Lewek Michael D. 
Published: April 2015

BackgroundFollowing stroke, spatiotemporal gait asymmetries persist into the chronic phases, despite the neuromuscular capacity to produce symmetric walking patterns. This persistence of gait asymmetry may be due to deficits in perception, as the newly established asymmetric gait pattern is perceived as normal.ObjectiveThe purpose of this study was to determine the effect of usual overground gait asymmetry on the ability to consciously and unconsciously perceive the presence of gait asymmetry in people poststroke.DesignAn observational study was conducted.MethodsThirty people poststroke walked overground and on a split-belt treadmill with the belts moving at different speeds (0%–70% difference) to impose varied step length and stance time asymmetries. Conscious awareness and subconscious detection of imposed gait patterns were determined for each participant, and the asymmetry magnitudes at those points were compared with overground gait.ResultsFor both spatial and temporal asymmetry variables, the asymmetry magnitude at the threshold of awareness was significantly greater than the asymmetry present at the threshold of detection or during overground gait. Participants appeared to identify belt speed differences using the type of gait asymmetry they typically exhibited (ie, step length or stance time asymmetries during overground gait).LimitationsVery few individuals with severe spatiotemporal asymmetry were tested, and participants were instructed to identify asymmetric belt speeds rather than interlimb movements.ConclusionsThe data suggest that asymmetry magnitudes need to exceed usual overground levels to reach conscious awareness. Therefore, it is proposed that the spatiotemporal asymmetry that is specific to each participant may need to be augmented beyond what he or she usually has during walking in order to promote awareness of asymmetric gait patterns for long-term correction and learning.
Optical sectioning and 3D reconstructions as an alternative to scanning electron microscopy for analysis of cell shape
Authors: Landis Jacob B. , Ventura Kayla L. , Soltis Douglas E. , Soltis Pamela S. , Oppenheimer David G. 
Published: April 2015

Premise of the study:Visualizing flower epidermal cells is often desirable for investigating the interaction between flowers and their pollinators, in addition to the broader range of ecological interactions in which flowers are involved. We developed a protocol for visualizing petal epidermal cells without the limitations of the commonly used method of scanning electron microscopy (SEM).Methods:Flower material was collected and fixed in glutaraldehyde, followed by dehydration in an ethanol series. Flowers were dissected to collect petals, and subjected to a Histo‐Clear series to remove the cuticle. Material was then stained with aniline blue, mounted on microscope slides, and imaged using a compound fluorescence microscope to obtain optical sections that were reconstructed into a 3D image.Results:This optical sectioning method yielded high‐quality images of the petal epidermal cells with virtually no damage to cells. Flowers were processed in larger batches than are possible using common SEM methods. Also, flower size was not a limiting factor as often observed in SEM studies. Flowers up to 5 cm in length were processed and mounted for visualization.Conclusions:This method requires no special equipment for sample preparation prior to imaging and should be seen as an alternative method to SEM.
L’éthique du cas : est-il possible de se régler sur l’éthique de la psychanalyse ?
Authors: Sauret Marie-Jean , Zapata-Reinert Luz 
Published: April 2015

Cet article vise à montrer en quoi opter pour une clinique orientée par la psychanalyse ne va pas sans un choix éthique. L’éthique venant là où aucun Autre ne répond, le désir de l’analyste se place ici comme opérateur d’une clinique où le savoir et l’acte sont sans cesse interrogés dans une démarche d’accueil de l’indécidable. Le paradigme de la singularité et la construction psychanalytique du cas sont développées afin de montrer que « faire cas » de la rencontre clinique consiste à s’intéresser aux effets de son intervention, à permettre au sujet de tirer les conséquences de ce qu’il y rencontre comme réel, à se faire passeur de ce que l’on y apprend. Le cas est ainsi replacé dans sa fonction de transmission.
Entre non-dits et « ça-voir », l’enfant, sujet de l’inconscient : un parti-pris éthique
Authors: Taverna Isabelle 
Published: April 2015

L’inconscient, le sujet du désir et le fantasme : voilà quelques jalons qui peuvent faire repères quant à notre éthique de travail. Cependant, quand nous travaillons avec un enfant, comment conjuguer ces repères avec les spécificités que nous imposent l’état d’enfance ? En effet, si les parents sont des acteurs avec qui le psychanalyste aura à composer, il considérera néanmoins l’enfant comme un Sujet à part entière et c’est du Sujet du désir et de l’inconscient dont il fera l’hypothèse. C’est cette éthique qui soutiendra son travail avec l’enfant. Tenant compte de ces différents paramètres, c’est une question plus spécifique que je souhaiterais déployer : Comment travailler lorsque nous sommes dépositaire d’un savoir sur son histoire, à l’insu de l’enfant ?