bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanotechnological Advances in the Diagnosis of Gynecological Cancers and Nanotheranostics
Authors: Keyvani Vahideh , Mollazadeh Samaneh , Riahi Espanta , Mahmoudian Reihaneh Alsadat , Anvari Kazem , Avan Amir 
Published: July 2024

:
Gynecological cancers are one of the main causes of female mortality worldwide. Despite the various
strategies to reduce mortality and improve quality of life, there are still many deficiencies in the diagnosis
and treatment of gynecological cancers. One of the important steps to ensure optimal cancer treatment is the
early detection of cancer cells and the use of drugs to reduce toxicity. Due to the increase in systemic toxicity
and resistance to traditional and conventional diagnostic methods, new strategies, including nanotechnology,
are being used to improve diagnosis and reduce the severity of the disease. Nanoparticles (NPs) provide exciting
opportunities to improve Gynecological Cancers (GCs) diagnosis, particularly in the initial stages. In
biomedical investigations and clinical settings, NPs can be used to increase the sensitivity and specificity of
recognition and/or imaging of GCs with the help of their molecular and cellular processes. To design more efficient
diagnostic NPs for gynecological cancer cells or tissues, determining the specific biomarkers is of great
importance. NP-based imaging agents are another solution to trace cancer cells. This review highlights the potential
of some NP-based diagnostic techniques in GC detection, which could be translated to clinical settings
to improve patient care.
Exploring the Potential of Avenanthramides and their Analogues as α-Glucosidase Inhibitors for Type 2 Diabetes Treatment Utilizing Virtual Screening, Molecular Dynamics, and Drug-likeness Predictions
Authors: Guendouze Assia , Mokrani El Hassen , Benslama Ouided , Lekmine Sabrina 
Published: July 2024

Background:
Optimal glycemic control is crucial in type 2 diabetes treatment, with αglucosidase inhibitors emerging as promising candidates. Avenanthramides, compounds found in
oats, exhibit therapeutic potential, but their efficacy as α-glucosidase inhibitors requires thorough
evaluation.

Objective:
This study investigates the potential of avenanthramides and their derivatives as inhibitors
of α-glucosidase for the treatment of type 2 diabetes through comprehensive computational analysis.

Methods:
Structure-Based Virtual Screening of 3543 avenanthramides and their analog compounds
was conducted using FlexX. The top 30 ranked compounds were subjected to visual inspection of
their binding mode within the α-glucosidase binding site to eliminate false positives. The top-ranked
molecule was subjected to dynamic simulation and ADMET prediction.

Results:
The results revealed that 1634 compounds were found to exhibit a greater α-glucosidase
inhibitory potency than miglitol, the reference molecule. Compound S1 exhibited superior αglucosidase inhibitory potency with a binding energy of -45.7786 kJ/mol compared to miglitol,
which had a binding energy of -26.5186 kJ/mol. S1 was predicted to occupy the entire binding site
with an optimized number of hydrogen bonds and hydrophobic interactions. Molecular dynamics
simulations demonstrated that S1 had a lower average RMSD (0.15 ± 0.01 nm) compared to miglitol
(0.16 ± 0.01 nm), indicating superior stability within the α-glucosidase binding site. S1 exhibited
favorable drug-like properties, suggesting its potential as a lead compound for further development
in type 2 diabetes treatment.

Conclusion:
These findings highlight S1's potential for diabetes treatment and pave the way for future experimental investigations. The computational approach utilized offers valuable insights into
the inhibitory potential of avenanthramides, providing a foundation for further drug development for
type 2 diabetes.
Therapeutic Effect of Unmodified Gold-platinum Nanoparticles on Gouty Arthritis Via the Nrf2/HO-1 Pathway
Authors: Liu Zirui , Li Dan , Zhao Baofeng , Mei Xifan 
Published: July 2024

Background:
Gouty arthritis, characterized by excruciating pain and discomfort,
poses a significant burden on patients. While nanomedicines have shown promise in addressing
this ailment, their complicated synthesis processes often involve potentially toxic procedures,
contributing to adverse side effects in disease management.

Methods:
In this study, we introduce a straightforward and elegant solution by utilizing easily
prepared gold platinum (AuPt) nanoparticles for the treatment of gouty arthritis. The synthesis of
these nanoparticles involves the use of gold and platinum precursors in conjunction with
NaBH4, simplifying the manufacturing process. Experimental models of gout were established
in both in vivo and in vitro settings through lipopolysaccharide and monosodium urate crystal
induction.

Results:
Our findings revealed that AuPt nanoparticles exhibited potent anti-inflammatory effects
against gout. This effect was attributed to their ability to activate the Nrf2/HO-1 pathway,
resulting in pain alleviation and the inhibition of inflammation, ultimately leading to the reduction
of joint edema. With their uncomplicated synthesis and promising therapeutic potential,
these simply prepared AuPt nanoparticles emerge as a compelling candidate for pharmaceutical
intervention in the treatment of gouty arthritis.

Conclusion:
This approach not only holds the promise of delivering effective relief to patients
but also minimizes the risk of unwanted side effects associated with complex nanomedicine
synthesis processes.
METTL3-mediated m6A Modification of hsa_circ_0131922 Attenuates the Progression of Papillary Thyroid Cancer by Regulating the p53 Pathway
Authors: Li Chan , Xie Ping , Lv Kun , Yang Qian , Mou Yanjie 
Published: July 2024

Aims:
This study aimed to confirm the regulatory role and mechanism of circular
RNA (circRNA) hsa_circ_0131922 in Papillary Thyroid Carcinoma (PTC) progression.

Background:
Accumulating evidence suggests that N6-methyladenosine (m6A)-modified circular
RNAs (circRNAs) perform pivotal functions in various malignancies. However, the specific
role of the m6A modification of circRNA mediated by METTL3 in Papillary Thyroid Carcinoma
(PTC) remains undocumented.

Objective:
In this work, we aimed to examine the molecular mechanisms of a novel m6Amodified
circRNA, hsa_circ_0131922, in PTC progression.

Methods:
Potential circRNA was identified from GEO datasets. The RNA or protein levels of
hsa_circ_0131922, METTL3, p53, and p21 were evaluated by qRT-PCR or western blot assays.
The various cellular functions were checked by CCK8, wound healing, transwell, and xenograft
tumor assays. MeRIP-qPCR was performed to observe the METTL3-mediated m6A modification
of hsa_circ_0131922. Furthermore, the interactions between hsa_circ_0131922 and
METTL3 in PTC were analyzed by bioinformatics analysis and various rescue experiments.

Results:
The levels of hsa_circ_0131922 were markedly downregulated in PTC tissues and cell
lines. In addition, the lower hsa_circ_0131922 levels correlated with poor prognosis in PTC
patients. The hsa_circ_0131922 overexpression reduced the malignant phenotypes of PTC cells
and activated the p53/p21 pathway. Bioinformatic analysis showed the m6A-modified sites of
hsa_circ_0131922, and a positive correlation between hsa_circ_0131922 and METTL3. Moreover,
overexpression of METTL3 increased the levels of m6A modification of
hsa_circ_0131922. Mechanistically, the anti-tumor effects of hsa_circ_0131922 overexpression
have been found to be partially reversed by silencing METTL3 in vivo and in vitro.

Conclusion:
The results have demonstrated m6A-modified hsa_circ_0131922 by METTL3 to
attenuate the progression of PTC by regulating the p53 pathway. Therefore, hsa_circ_0131922
could be a predictive prognostic biomarker and therapeutic target for PTC.
Mediating Role of Psychological Safety on Support at the Workplace and its Association with Organization Performance: Context of Indian IT Sector
Authors: Jindal Priya , Mittal Amit , Gupta Piyush , Chaudhry Rishi 
Published: July 2024

Introduction
In India, the IT sector has grown to be a substantial industry. Emerging technologies demonstrate their capability to offer clients globally, not only on-shore but also off-shore services. The workplace evolution of the IT sector, which includes a highly structured dynamic system and cutting-edge technology, makes the employees experience anxiety, trepidation, or a general unease while interacting socially or personally with their coworkers. Employees may have anxiety over their capacity to sustain themselves, hold onto their position within the company, and be viewed as valuable contributors by superiors and peers. As the work pressure increases, this helps the organization to understand the impression of psychological safety.

Methods
The current study is an attempt to delve into the association between social support, i.e., Perceived Organizational Support (POS), Perceived Supervisory Support (PSS), and Perceived Co-worker Support (PCS), and personal employee support, i.e., Personal Self-Concept (PSC) at the workplace and Organizational Performance (OP). The study also analyzes the mediating role of Psychological Safety (PS) in this relationship. A cross-sectional survey was conducted to collect data from 232 Indian IT professionals. The data were analyzed using the SPSS statistical software package with Covariance-Based Structural Equation Modeling (CB-SEM). Evaluation of the hypotheses was conducted through the SEM, and mediation analysis was carried out using bootstrap estimation in SPSS AMOS.

Results
The results suggest that POS and PSS have a significant effect on PS and OP. Also, PS has a direct impact on OP. Mediation analysis suggests that PS has full mediation between the relationship of PSC and OP while partial mediation between PSS and OP.

Conclusion
To stay competitive and gain an edge in a continuously changing environment, IT organizations are looking to execute strategies that will help them retain their personnel. Therefore, businesses are keen to provide their employees with psychological safety and a sense of support so that they can perform more effectively in the workplace. Regarding psychological safety, this affects not only the individual but also teams and, thus, the entire company.
Determinants of Small and Rural Local Governments’ Renewable Energy Program Adoption in Cascadia
Authors: Steel Brent Steven , Wolters Erika Allen , Krawchenko Tamara , Farooq Sadaf 
Published: July 2024

Aim:
This study aimed to investigate the determinants of renewable energy policy
adoption by small and rural local governments in Cascadia.

Background:
Small and rural local governments currently face many ongoing and numerous new
challenges that complicate their task of sustaining current public services and programs. How
government officials adapt to these changes can affect the long-term viability of local governments in both the U.S. and Canadian contexts.

Objective:
This study has examined the presence or absence of renewable energy programs in
small and rural local governments in the “Cascadia” region of Canada and the U.S. (British Columbia, Oregon, and Washington).

Methods:
Using surveys and interviews of Cascadia local government leaders during the summer
and fall of 2023, correlates of renewable energy policy adoption have been examined, including
cultural, demographic, economic, and political factors.

Results:
Key findings have indicated cities, experiencing population growth, and those with a
progressive political orientation to be more inclined to adopt renewable energy policies. Conversely, remote communities have demonstrated a lower propensity for such adoption. Financial
constraints, evidenced by the impact of inflation and the necessity for service cutbacks, have
been found to negatively correlate with the consideration of renewable energy policies.

Conclusion:
This study has indicated renewable energy projects to be more often found or contemplated in areas being politically liberal, densely populated, and not predominantly rural. It
could be beneficial in shifting the perception of renewable energy from being predominantly an
environmental concern to being recognized for its economic benefits.
Acupuncture as Adjuvant Therapy for Glaucoma: Protocol for a Randomized Controlled Trial
Authors: Liao Yi-Fang , Lee Yu-Chen , Lin Hui-Ju , Shao Yi-Ching 
Published: July 2024

Background
Glaucoma is a chronic progressive optic neuropathy that necessitates lifelong treatment to reduce the decline of the optic nerve. Due to the extended and continuous treatments required for patients, complementary therapies are often considered alongside conventional treatments to enhance the effectiveness of the treatment. Acupuncture has demonstrated the potential to lower intraocular pressure in previous clinical trials, making it a promising glaucoma intervention.

Objective
The primary objective of this study is to conduct a single-center randomized control trial involving patients with glaucoma. Acupuncture will be evaluated as an adjunctive therapy. The trial aims to explore its effectiveness for glaucoma.

Methods
In this single-center randomized controlled trial, participants (N=50) with primary open-angle glaucoma will be randomly assigned to the treatment group, receiving ophthalmic acupuncture with “De Qi” sensation, or the control group, receiving minimum acupuncture stimulation on nonophthalmic acupoints. The intervention will consist of weekly acupuncture treatments for a total of 6 sessions. Participants will be assessed at 8 time points, which are baseline, during the intervention (6 times), and at a 3-month follow-up. The primary outcome measure is a change in the intraocular pressure before and after each acupuncture treatment. Secondary outcomes will include measurements of heart rate and blood pressure before and after acupuncture, best-corrected visual acuity, visual field, optical coherence tomography, optical coherence tomography angiography, the Glaucoma Symptom Scale, and the Glaucoma Quality of Life-15 questionnaire.

Results
Recruitment of participants for the trial commenced on June 28, 2023. A total of 10 participants have been enrolled to test the feasibility of the experiment. We anticipate that the preliminary data from this trial will be completed by December 2025.

Conclusions
This trial uses rigorous methodology and comprehensive outcome measurements to assess the clinical efficacy of acupuncture as an adjunctive therapy for glaucoma, providing valuable insights for future clinical treatment guidelines.

Trial Registration
ClinicalTrials.gov NCT05753137; https://clinicaltrials.gov/study/NCT05753137

International Registered Report Identifier (IRRID)
DERR1-10.2196/57888
Quantification of Urinary Exosomal Prostate-Specific Antigen for the Diagnosis of Prostate Cancer Using Clinical Laboratory–Based Techniques: Protocol for a Case-Control Study
Authors: Li Guorong , Tholance Yannick , Mallouk Nora , Waeckel Louis , Flandrin Pascale , Bali Bruno , Badet Lionel , Cornillon Pierre 
Published: July 2024

Background
Prostate cancer is the most common cancer in men and represents a major public health problem. The current method for the diagnosis or screening of prostate cancer is invasive and costly. There have been renewed and innovative studies searching for urinary biomarkers to aid in the diagnosis of prostate cancer, especially with technologies based on urinary exosomes. However, technologies based on urine exosomes usually need expensive machines such as an ultracentrifuge and they are difficult to standardize, which hinder their application in clinical laboratories. We have optimized and standardized the isolation of urinary exosomes with the precipitation method. We have found that urinary exosomal prostate-specific antigen (PSA) can be quantified by automatic Elecsys total PSA technique.

Objective
In this study, our objective is to utilize urinary exosomes from prostate cancer for the development of a test to aid in its diagnosis.

Methods
Exosomes from the prostate cancer cell line LNCaP was used to set up the technique. To analyze urine samples from patients, the methods include the collection of first-void urine using the Colli-Pee device, the isolation of urine exosomes using the optimized precipitation method, and the quantification of exosomal PSA by Elecsys total PSA.

Results
This will be a 2-year study. We will start including patients and controls in the last quarter of 2024. We expect the results to be published in the second quarter of 2027.

Conclusions
This is the first study to quantify urinary exosomal PSA using the Elecsys total PSA technique for the diagnosis of prostate cancer. This study emphasizes techniques that are suitable for implementation in clinical laboratories, which will facilitate the application of urinary exosomes to simplify and improve the diagnosis and screening of prostate cancer.

International Registered Report Identifier (IRRID)
PRR1-10.2196/63551
Identification of Betulinic Acid and Trimethoxyellagic Acid as the Antidiabetic Compounds in Anogeissus leiocarpus Stem Bark Purified Extract
Authors: Esievo King Akpofure Nelson , Balogun Emmanuel Oluwadare , Esievo Kingsley Oghenerukevwe , Esievo Lauretta Oghenekevwe , Esievo Edith Monica , Sani Dahiru , Wassagwa John , Uyovbisere Edward Oniovosa , Mumah Emmanuel Tamajong 
Published: July 2024

Objectives: The study aimed to identify the antidiabetic compounds purified from stem bark of Anogeissus leiocarpus and propose the mechanisms of action.
Design: Anogeissus leiocarpus stem bark was purified through ethyl acetate and n-hexane with minor exceptions. For very clear separation, cold acetone was added to trigger the precipitation. The precipitate was dissolved with a mixture of DCM:methanol (9:1), adsorbed it to silica gel (5 g), evaporated to free flowing powder and fractionated it over silica gel (50 g) to realize 40 fractions. The gummy fractions were ignored. The light brown powder which possessed antidiabetic effect was selected for Nuclear Magnetic Resonance for structural elucidation.
Nuclear Magnetic Resonance (NMR) and Determination of Structure of Purified Compound: After column chromatography and TLC processes, along with the cold acetone, to the point of crystallization, the purified compounds, the light brown powder, were presented to NMR (Bruker Avance III, Spectrometer frequency 400 MHz; solvents DMSO-do, CDCl3, Acetone-d6. Institute of Chemistry, Strathclyde University Glasgow UK.) for determination of compound structures and analyses.
Results: The NMR spectra and analyses revealed the existence of Betulinic acid and Trimethoxyellagic acid.
Conclusion: We show that Betulinic acid and Trimethoxyellagic acid are potent antidiabetic compounds in the stem bark extract of A. leiocarpus.
Keywords: Anogeissus leiocarpus, Betulinic Acid, Trimethoxyellagic Acid, Antidiabetic agent
The MeViDa Study: Bioequivalence Study of FDC of Dapagliflozin, Vildagliptin SR and Metformin SR in Healthy Indian Volunteers: A Randomized, Open-Label, Crossover Study
Authors: Pednekar Abhijit , Gupta Amit , Warrier Sona 
Published: July 2024

Objective: To assess the pharmacokinetics and bioequivalence of Dapagliflozin 10mg + Vildagliptin Sustained Release (SR) 100mg + Metformin SR 1000mg fixed dose combination (FDC) tablets with DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin SR 1000 mg tablets) in healthy adult male subjects under fasting conditions.
Material and Methods: This was an open-label, balanced, randomized, two-treatment, two-period, two-sequence, single-dose, crossover, oral bioequivalence study. Volunteers were randomized to receive either a test product or a reference product under the fasting condition with a seven-day washout period. The pharmacokinetics parameters evaluated were maximum plasma concentration (Cmax), the area under the curve (AUC) at time t (AUC0-t), and the total area under the curve (AUC0-∞). Adverse events were also assessed as safety endpoints.
Results: Twenty-four healthy adult male subjects were randomized, and 24 completed the study. The mean values for Cmax, AUC0-t, and AUC0-∞ were almost identical for test and reference products. The 90% confidence intervals of the ratios of adjusted geometric means for the pharmacokinetic parameters, i.e. Cmax, AUC0-t, and AUC0-∞ of the test product, were within the predefined bioequivalence limits of 80.00 to 125.00%. No adverse events or serious adverse events or deaths were reported during the study. All treatments were well tolerated.
Conclusion: The test formulation, Dapagliflozin 10mg + Vildagliptin SR 100mg + Metformin SR 1000mg FDC, were bioequivalent to DAPAMAC V 10 (Dapagliflozin 10mg + Vildagliptin SR 100mg tablets) and Glycomet 1g (Metformin 1000mg SR tablets) in healthy adult male subjects under fasting conditions.
Keywords: Bioequivalence, Fixed-Dose Combination, Dapagliflozin, Metformin, Sustained Release, Vildagliptin, AUC, Cmax
Method development and validation for estimation of Gliclazide in bulk and tablet form by UV Spectrophotometer
Authors: Hanwate Ravindra , Singh Bhupendra , Khairnar Nilesh , Chavan Sushmita 
Published: July 2024

UV Spectrophotometric method has been developed to determine Gliclazide in bulk pure and tablet forms. It is a simple, accurate, reproducible, rapid and less time-consuming method. The maximum wavelength of the drug was found to be 232nm. Beer Lamberts’ law was obeyed in the concentration range of 2-20 µg/ml. (LOD)The limit of detection and limit of qualification (LOQ) was found to be 0.16 µg/ml and 0.50 µg/ml from this method per cent recovery of the drug was found to be 99.30% which indicates no interaction of the excipients. This method was found accurate, simple, precise and rapid for determination of tablet dosage form.
Keywords: validation, Gliclazide, tablets, UV Spectroscopy, LOD, LOQ
PELATIHAN PEMBUATAN PUPUK ORGANIK GRANUL (POG) BERBAHAN BAKU SLUDGE LUARAN DIGESTER BIOGAS SEBAGAI UPAYA PENGOPTIMALAN PEMANFAATAN DIGESTER BIOGAS PEDESAAN
Authors: Damayanti Sri Ismiyati , Nawansih Otik , Iryani Dewi Agustina , Taharuddin Taharuddin , Haviz Muhammad , Ginting Simparmin Br 
Published: July 2024

Pupuk bioslurry padat (sludge) luaran digester biogas mempunyai kualitas yang bagus, namun masih kurang menarik bagi petani karena berbentuk curah. Oleh karena itu, perlu dilakukan pelatihan terkait granulasi sludge tersebut menjadi pupuk organik granul (POG). Tujuan jangka panjang pengabdian ini adalah kemandirian pupuk di tingkat petani, melalui kemampuan petani mengolah limbah di sekitarnya menjadi pupuk organik yang lebih praktis. Target khususnya adalah masyarakat mengetahui iptek terkait granulasi pupuk dan mempunyai keterampilan dalam menggranulkan pupuk. Metode yang digunakan adalah : koordinasi tim dosen dengan warga terkait kegiatan pelatihan, penyiapan bahan baku, yaitu sludge dan kompos berbahan baku sludge-kulit kopi, sosialisasi dan diskusi terkait iptek granulasi pupuk, pelatihan pembuatan POG, dan evaluasi kegiatan bersama masyarakat. Pelaksanaan pengabdian diawali dengan koordinasi tim dosen dengan warga terkait pelaksanaan kegiatan, dilanjutkan penyiapan bahan baku dan alat dengan dibantu warga dan mahasiswa, pelaksanaan pelatihan dibantu mahasiswa dengan mengundang aparat pekon dan warga, diakhiri dengan evaluasi bersama warga untuk menentukan keberlanjutan program. Masyarakat sangat tertarik menjadikan pupuk organik ini sebagai salah satu usaha desa, yang ke depannya dapat dikelola BUMDes. Kendala selama pengabdian adalah sulitnya mengumpulkan warga di siang hari karena berbagai kesibukan yang berbeda. Warga di desa biasa berkumpul malam hari jika ada yang akan dibicarakan