bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing In Vivo Survival of Adipose-Derived Stromal Cells Through Bcl-2 Overexpression Using a Minicircle Vector
Authors: Hyun Jeong , Grova Monica , Nejadnik Hossein , Lo David , Morrison Shane , Montoro Daniel , Chung Michael , Zimmermann Andrew , Walmsley Graham G. , Lee Min , Daldrup-Link Heike , Wan Derrick C. , Longaker Michael T. 
Published: August 2013

Abstract
Tissue regeneration using progenitor cell-based therapy has the potential to aid in the healing of a diverse range of pathologies, ranging from short-gut syndrome to spinal cord lesions. However, there are numerous hurdles to be overcome prior to the widespread application of these cells in the clinical setting. One of the primary barriers to effective stem cell therapy is the hostile environment that progenitor cells encounter in the clinical injury wound setting. In order to promote cellular survival, stem cell differentiation, and participation in tissue regeneration, relevant cells and delivery scaffolds must be paired with strategies to prevent cell death to ensure that these cells can survive to form de novo tissue. The Bcl-2 protein is a prosurvival member of a family of proteins that regulate the mitochondrial pathway of apoptosis. Using several strategies to overexpress the Bcl-2 protein, we demonstrated a decrease in the mediators of apoptosis in vitro and in vivo. This was shown through the use of two different clinical tissue repair models. Cells overexpressing Bcl-2 not only survived within the wound environment at a statistically significantly higher rate than control cells, but also increased tissue regeneration. Finally, we used a nonintegrating minicircle technology to achieve this in a potentially clinically applicable strategy for stem cell therapy.
Direct-to-Consumer Stem Cell Marketing and Regulatory Responses
Authors: Sipp Douglas 
Published: August 2013

Summary
There is a large, poorly regulated international market of putative stem cell products, including transplants of processed autologous stem cells from various tissues, cell processing devices, cosmetics, and nutritional supplements. Despite the absence of rigorous scientific research in the form of randomized clinical trials to support the routine use of such products, the market appears to be growing and diversifying. Very few stem cell biologics have passed regulatory scrutiny, and authorities in many countries, including the United States, have begun to step up their enforcement activities to protect patients and the integrity of health care markets.
Intravenous Administration of Human Umbilical Cord Blood-Derived AC133+ Endothelial Progenitor Cells in Rat Stroke Model Reduces Infarct Volume: Magnetic Resonance Imaging and Histological Findings
Authors: Iskander Asm , Knight Robert A. , Zhang Zheng Gang , Ewing James R. , Shankar Adarsh , Varma Nadimpalli Ravi S. , Bagher-Ebadian Hassan , Ali Meser M. , Arbab Ali S. , Janic Branislava 
Published: August 2013

AbstractEndothelial progenitor cells (EPCs) hold enormous therapeutic potential for ischemic vascular diseases. Previous studies have indicated that stem/progenitor cells derived from human umbilical cord blood (hUCB) improve functional recovery in stroke models. Here, we examined the effect of hUCB AC133+ EPCs on stroke development and resolution in a middle cerebral artery occlusion (MCAo) rat model. Since the success of cell therapies strongly depends on the ability to monitor in vivo the migration of transplanted cells, we also assessed the capacity of magnetic resonance imaging (MRI) to track in vivo the magnetically labeled cells that were administered. Animals were subjected to transient MCAo and 24 hours later injected intravenously with 107 hUCB AC133+ EPCs. MRI performed at days 1, 7, and 14 after the insult showed accumulation of transplanted cells in stroke-affected hemispheres and revealed that stroke volume decreased at a significantly higher rate in cell-treated animals. Immunohistochemistry analysis of brain tissues localized the administered cells in the stroke-affected hemispheres only and indicated that these cells may have significantly affected the magnitude of endogenous proliferation, angiogenesis, and neurogenesis. We conclude that transplanted cells selectively migrated to the ischemic brain parenchyma, where they exerted a therapeutic effect on the extent of tissue damage, regeneration, and time course of stroke resolution.
Changement des états de surface, précipitations automnales et vulnérabilité des sols dans le bassin versant du Bouregreg au Maroc
Authors: Brou Yao Télesphore , Emram Anas , Abdellah Laouina , Chaker Miloud , Coupleux Sylvie , Boujrouf Saïd 
Published: August 2013

Localisé dans la région de Rabat-Salé-Zemour, le bassin versant du Bouregreg est un espace essentiellement pastoral dans ses parties intérieures montagneuses et une zone agro-pastorale en aval. Les dynamiques paysagères observées sur ce bassin versant, à l’instar de la plupart des bassins versants du Maroc atlantique, sont marquées par une densification des zones de cultures dans les plaines et de leur extension sur les versants. La classification supervisée des images Landsat de 1987 et 2006 a permis de mettre en évidence l’évolution de l’occupation du sol. Sur l’ensemble du bassin versant, entre 1987 et 2006, 15 % des surfaces boisées ont été transformées en espaces agricoles. La prise en compte de la morphologie du bassin versant permet de mettre en évidence la fragilité des paysages agricoles. En effet, près de 39 % des surfaces cultivées sont situées sur des pentes de 5 à 10 % et près de 24 % sur les pentes de 10 à 20 %.
Ces terres de culture présentent une quasi-absence de végétation durant la période de préparation des sols et de semis. L’archivage et le suivi à l’échelle saisonnière des NDVI (indice de végétation normalisé) décadaires du capteur Spot végétation sur la période 2001-2009 révèlent un allongement de la période au cours de laquelle les sols sont quasi-dénudés durant les années de sécheresses exceptionnelles.
Par ailleurs, l’analyse de la série pluviométrique de la station de Rabat met en évidence une modification du régime des pluies automnales à partir de 1999, marquée notamment par une tendance à l’accroissement des hauteurs de pluie en octobre. Cette situation est susceptible d’accélérer la vulnérabilité des sols dénudés (à pentes fortes) à l’érosion pluviale, notamment à l’occasion d’années particulièrement sèches comme ce fut le cas en 2003 et 2007.
Oxidative CO Cross‐Coupling of 1,3‐Dicarbonyl Compounds and Their Heteroanalogues with N‐Substituted Hydroxamic Acids and N‐Hydroxyimides
Authors: Terent'ev Alexander O. , Krylov Igor B. , Timofeev Vladimir P. , Starikova Zoya A. , Merkulova Valentina M. , Ilovaisky Alexey I. , Nikishin Gennady I. 
Published: August 2013

AbstractThe oxidative CO cross‐coupling of 1,3‐dicarbonyl compounds and their heteroanalogues, 2‐substituted malononitriles and cyanoacetic esters, with N‐substituted hydroxamic acids and N‐hydroxyimides was realized. The best results were obtained with the use of manganese(III) acetate [Mn(OAc)3] or the cobalt(II) acetate catalyst [Co(OAc)2cat.]/potassium permanganate [KMnO4] system as the oxidant. The synthesis can be scaled up to gram quantities of coupling products; yields are 30–94%. The reaction proceeds via a radical mechanism through the formation of nitroxyl radicals from N‐substituted hydroxamic acids and N‐hydroxyimides.magnified image
Electrochemical Reaction of Lithium with Nanostructured Silicon Anodes: A Study by In‐Situ Synchrotron X‐Ray Diffraction and Electron Energy‐Loss Spectroscopy
Authors: Wang Feng , Wu Lijun , Key Baris , Yang Xiao‐Qing , Grey Clare P. , Zhu Yimei , Graetz Jason 
Published: August 2013

AbstractSilicon‐based anodes are an appealing alternative to graphite for lithium‐ion batteries because of their extremely high capacity. However, poor cycling stability and slow kinetics continue to limit the widespread use of silicon in commercial batteries. Performance improvement has been often demonstrated in nanostructured silicon electrodes, but the reaction mechanisms involved in the electrochemical lithiation of nanoscale silicon are not well understood. Here, in‐situ synchrotron X‐ray diffraction is used to monitor the subtle structural changes occurring in Si nanoparticles in a Si‐C composite electrode during lithiation. Local analysis by electron energy‐loss spectroscopy and transmission electron microscopy is performed to interrogate the nanoscale morphological changes and phase evolution of Si particles at different depths of discharge. It is shown that upon lithiation, Si nanoparticles behave quite differently than their micrometer‐sized counterparts. Although both undergo an electrochemical amorphization, the micrometer‐sized silicon exhibits a linear transformation during lithiation, while a two‐step process occurs in the nanoscale Si. In the first half of the discharge, lithium reacts with surfaces, grain boundaries and planar defects. As the reaction proceeds and the cell voltage drops, lithium consumes the crystalline core transforming it into amorphous LixSi with a primary particle size of just a few nanometers. Unlike the bulk silicon electrode, no Li15Si4 or other crystalline LixSi phases were formed in nanoscale Si at the fully‐lithiated state.
Human linear growth trajectory defined
Authors: Mon Anderson , Cabana Michael , Halpern‐Felsher Bonnie , Meyerhoff Dieter Johannes 
Published: August 2013

ABSTRACTObjectivesThe purpose of this study was to assess the applicability of a simple mathematical formula for prediction of individual child linear growth. The formula describes a square root dependence of height on age with only two constants, k and C.MethodsRetrospective serial height measurements of 137 healthy children (61 female), who attended clinic in the Pediatrics Department at the University of California, San Francisco were used. For each child, two of the initial measurements and their corresponding measurement times were used to determine the values of k and C. By substituting the determined values of k and C into the formula, the formula was then used to predict the trajectory of the child's growth.ResultsThe 137 children were comprised of 20% Hispanic, 23% African‐American, 27% Caucasian and 30% Asian. The formula predicted growth trajectories of 136 out of the 137 children with minimal discrepancies between the measured data and the corresponding predicted data. The mean of the discrepancies was 0.8 cm.ConclusionsOur proposed formula is very easy to use and predicts individual child growth with high precision irrespective of gender or ethnicity. The formula will be a valuable tool for studying human growth and possibly growths of other animals. Am. J. Hum. Biol., 25:666‐672, 2013. © 2013 Wiley Periodicals, Inc.
TigarB causes mitochondrial dysfunction and neuronal loss in PINK1 deficiency
Authors: Flinn Laura J. , Keatinge Marcus , Bretaud Sandrine , Mortiboys Heather , Matsui Hideaki , De Felice Elena , Woodroof Helen I. , Brown Lucy , McTighe Aimee , Soellner Rosemarie , Allen Claire E. , Heath Paul R. , Milo Marta , Muqit Miratul M. K. , Reichert Andreas S. , Köster Reinhard W. , Ingham Philip W. , Bandmann Oliver 
Published: August 2013

ObjectiveLoss of function mutations in PINK1 typically lead to early onset Parkinson disease (PD). Zebrafish (Danio rerio) are emerging as a powerful new vertebrate model to study neurodegenerative diseases. We used a pink1 mutant (pink−/−) zebrafish line with a premature stop mutation (Y431*) in the PINK1 kinase domain to identify molecular mechanisms leading to mitochondrial dysfunction and loss of dopaminergic neurons in PINK1 deficiency.MethodsThe effect of PINK1 deficiency on the number of dopaminergic neurons, mitochondrial function, and morphology was assessed in both zebrafish embryos and adults. Genome‐wide gene expression studies were undertaken to identify novel pathogenic mechanisms. Functional experiments were carried out to further investigate the effect of PINK1 deficiency on early neurodevelopmental mechanisms and microglial activation.ResultsPINK1 deficiency results in loss of dopaminergic neurons as well as early impairment of mitochondrial function and morphology in Danio rerio. Expression of TigarB, the zebrafish orthologue of the human, TP53‐induced glycolysis and apoptosis regulator TIGAR, was markedly increased in pink−/− larvae. Antisense‐mediated inactivation of TigarB gave rise to complete normalization of mitochondrial function, with resulting rescue of dopaminergic neurons in pink−/− larvae. There was also marked microglial activation in pink−/− larvae, but depletion of microglia failed to rescue the dopaminergic neuron loss, arguing against microglial activation being a key factor in the pathogenesis.InterpretationPink1−/− zebrafish are the first vertebrate model of PINK1 deficiency with loss of dopaminergic neurons. Our study also identifies TIGAR as a promising novel target for disease‐modifying therapy in PINK1‐related PD. Ann Neurol 2013;74:837–847
Modified montmorillonite and its application as a flame retardant for polyester
Authors: Wang Jiaoning , Mao Zhiping 
Published: August 2013

ABSTRACTA novel flame retardant composed of montmorillonite (MMT) and hexachlorocyclotriphosphazene (HCCP) was prepared in this study. MMT was chemically bonded with HCCP via a 3‐aminopropyltriethoxysilane (KH‐550) coupling agent. The structure was characterized by Fourier transform infrared spectroscopy, X‐ray diffraction, and NMR. The preliminary application for improving the flame retardancy of poly(ethylene terephthalate) (PET) was investigated by means of the limited oxygen index, the vertical burning test, and thermogravimetric analysis. Scanning electron microscopy was used to investigate the morphology of the char residue. The results show that the novel flame retardant had excellent fire retardancy; that is, the sample achieved an increased UL‐94 V‐0 rating and limited oxygen index value of 31.5. The residue of the flame‐retarded PET increased to 14.7% compared to the 6.2% value of pristine PET. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39625.
Friction and wear properties of graphene oxide/ultrahigh‐molecular‐weight polyethylene composites under the lubrication of deionized water and normal saline solution
Authors: An Yingfei , Tai Zhixin , Qi Yuanyuan , Yan Xingbin , Liu Bin , Xue Qunji , Pei Jinying 
Published: August 2013

ABSTRACTUltrahigh‐molecular‐weight polyethylene (UHMWPE) and UHMWPE composites reinforced with graphene oxide (GO) were successfully fabricated through a new step of liquid‐phase ultrasonic dispersion, high‐speed ball‐mill mixing, and hot‐pressing molding technology. When the GO/UHMWPE composites were lubricated with deionized water (DW) and normal saline (NS) solution, their friction and wear properties were investigated through sliding against ZrO2. The worn surface and wear volume losses of these composites were studied with scanning electron microscopy, X‐ray photoelectron spectroscopy, and a Micro‐XAM 3D non‐contact surface profiler. The results show that the microhardness of the GO/UHMWPE composites was improved by 13.80% and the wear rates were decreased by 19.86 and 21.13%, whereas the depths of the scratches were decreased by 22.93 and 23.77% in DW and NS lubricating conditions, respectively. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39640.
Synthesis and characterization of novel pH‐responsive poly(2‐hydroxylethyl methacrylate‐co‐N‐allylsuccinamic acid) hydrogels for drug delivery
Authors: Ozay Ozgur 
Published: August 2013

ABSTRACTIn this study, N‐allylsuccinamic acid (NASA) was synthesized in a single step with a yield of 85%. Carboxylic acid containing NASA was characterized through Fourier transform infrared (FTIR) radiation and 1H‐NMR and 13C‐NMR analysis, and then it was used for synthesis of poly(2‐hydroxylethyl methacrylate‐co‐N‐allylsuccinamic acid) [p(HEMA‐co‐NASA)] hydrogels. The structure of the obtained pH‐responsive p(HEMA‐co‐NASA) hydrogels were characterized with FTIR spectroscopy and scanning electron microscopy analysis, and their swelling characterization was carried out under different drug‐release conditions. In the application step of the study, the hydrogels were used for the in vitro release of vitamin B12 and Rhodamine 6G, which were selected as model drugs. We determined that the hydrogels used as a drug‐delivery matrix could release the drug they had absorbed under different release conditions (phosphate‐buffered saline, 0.9% NaCl, and pH 1.2) at high rates for time periods of up to 24 h. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39660.
Preparation and properties of carbon nanotube/binary‐polymer composites with a double‐segregated structure
Authors: Pang Huan , Bao Yu , Yang Shu‐Gui , Chen Chen , Zhang Wei‐Qin , Chen Jun , Ji Xu , Lei Jun 
Published: August 2013

ABSTRACTA carbon nanotube (CNT)/poly(methyl methacrylate) (PMMA)/ultrahigh molecular weight polyethylene (UHMWPE) composite containing a double‐segregated structure was formalized by means of a facile mechanical mixing technology. In the composite, the CNTs were decorated on the surfaces of PMMA granules, and the CNTs decorated granules formed the continuous segregated conducting layers at the interfaces between UHMWPE particles. Morphology observations confirmed the formation of a specific double‐segregated CNT conductive network, resulting in an ultralow percolation threshold of ∼0.2 wt %. The double‐segregated composite containing only 0.8 wt % CNT loading exhibited a high electrical conductivity of ∼0.2 S m−1 and efficient electromagnetic shielding effectiveness of ∼19.6 dB, respectively. The thermal conductivity, temperature‐resistivity behaviors, and mechanical properties of the double‐segregated composites were also studied. This work provided a novel conductive network structure to attain a high‐performance conducting polymer composite at low filler loadings. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39789.
Complex temporal decay: a complete analysis
Authors: Quintero‐Torres R , Castillo‐Matadamas HA , Young Jeff F , Bermúdez Cruz RM 
Published: August 2013

ABSTRACTRelaxation dynamics is universal in science and engineering; its study serves to parameterize a system's response and to help identify a microscopic model of the processes involved. When measured data for a phenomenon cannot be fitted using one exponential, the choice of an alternative function to describe the decay becomes nontrivial. Here, we contrast two different, but fundamentally related approaches to fitting nontrivial decay curves; exponential decomposition and the gamma probability density function. Copyright © 2013 John Wiley & Sons, Ltd.
RDAP13 summit: Introduction
Authors: Parham Susan Wells , Rolando Elizabeth , Doty Jennifer 
Published: August 2013

AbstractEditor's SummaryThis special section of the Bulletin explores issues in research data access and preservation (RDAP) and samples the program highlights and attendees' reflections of the ASIS&T RDAP13 Summit, the 4th annual meeting, held in Baltimore, Maryland, on April 4–5, 2013. Contributed papers address the value of partnering with publishers and repositories as a way to address the complexity of data curation and explore the issue of data use and reuse from a data curator's perspective. One article discusses the use, benefits and challenges of altmetrics to track citations of research data. Others tackle the need to develop a standard data infrastructure and describe efforts to promote research data management across institutions of higher education in the United Kingdom. Last, a sampling of the posters presented at RDAP13 reflects the scope of the topic, current progress and advances to come.
The research data alliance: Implementing the technology, practice and connections of a data infrastructure
Authors: Parsons Mark A. 
Published: August 2013

AbstractEditor's SummaryWith research in demand for innumerable applications to society's challenges, the Research Data Alliance (RDA) was formed to create and implement a unified data infrastructure. Established in March 2013, the alliance is directed toward establishing a framework to serve social and technical research needs at the local and global levels. The RDA brings together a community of stakeholders to guide the organization as it evolves and align participants' diverse perspectives, striving for consensus, harmonization and an open community. Its structure features organizational and technical advisory boards, interest groups, shorter term working groups and an elected council to steer the alliance. With 700 members representing the academic, public and private sectors across 44 countries, the RDA seeks support from researchers, data managers, funding agencies and governments to promote the strategic and effective use of research data.
An Eco‐Friendly Enantioselective Access to (R)‐Naringenin as Inhibitor of Proinflammatory Cytokine Release
Authors: Gaggeri Raffaella , Rossi Daniela , Daglia Maria , Leoni Flavio , Avanzini Maria Antonia , Mantelli Melissa , Juza Markus , Collina Simona 
Published: August 2013

Abstract(RS)‐Naringenin is a flavanone well‐known for its beneficial health‐related properties, such as its anti‐inflammatory activity. The preparative enantioselective chromatographic resolution of commercial (RS)‐naringenin was performed on a Chiralpak AD‐H column (500×50 mm i.d., dp 20 μm) using MeOH as eluent. The developed method is in accordance with the principles of green chemistry, since the environmental impact was lowered by recycling of the eluent, and allowed the production of gram amounts of each enantiomer with high purity (chemical purity >99%, enantiomeric excess (ee) >94%). Racemic and enantiomeric naringenin were subjected to an exhaustive in vitro investigation of anti‐inflammatory activity, aimed at evaluating the relevance of chirality. The assay with cultured human peripheral blood mononuclear cells (hPBMC) activated by phytohemagglutinin A revealed that (R)‐naringenin was more effective in inhibiting T‐cell proliferation than the (S)‐enantiomer and the racemate. Moreover, (R)‐naringenin significantly reduced proinflammatory cytokine levels such as those of TNF‐α and, with less potency, IL‐6. These results evidenced the anti‐inflammatory potential of naringenin and the higher capacity of (R)‐naringenin to inhibit both in vitro hPBMC proliferation and cytokine secretion at non toxic doses. Thus, (R)‐naringenin is a promising candidate for in vivo investigation.