bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ethiopian women's tokophobia of childbirth and its predictors: a systematic review and meta-analysis
Authors: Assimamaw Nega Tezera , Gonete Almaz Tefera , Angaw Dessie Abebaw , Techane Masresha Asmare , Kelkay Mengistu Mekonnen , Terefe Bewuketu 
Published: August 2024

BackgroundFear of childbirth is recognized as a growing problem in developing countries, including Ethiopia. The impact of this fear on women's reproductive choices and decisions is significant. Therefore, the systematic review and meta-analysis will help to consolidate the existing research on childbirth-related fear in Ethiopia. Synthesizing the findings and providing a pooled prevalence estimate, can contribute to a better understanding of the scale of the problem in the country.ObjectiveThis systematic review and meta-analysis assessed the pooled prevalence of childbirth-related fear and its associated factors among pregnant mothers in Ethiopia.MethodsPubMed, Google Scholar, and African Journals Online were searched for included articles. A weighted inverse-variance random-effects model was used to estimate the prevalence of childbirth-related fear. Variations in the pooled estimates of the prevalence were adjusted through subgroup analysis according to the specific region where the study was conducted. Funnel plot and Egger's regression test were used to check for publication bias. STATA version 14 statistical software was used for meta-analysis.ResultsA total of 2,015 pregnant mothers were included. The combined prevalence of fear of childbirth among pregnant mothers was found to be 21% (95% CI: 19–22; I2 = 0.00%, p value &lt; 0.001). Based on the subgroup analysis, the prevalence of fear of childbirth among pregnant mothers was 24% in SNNPRs, 25% in Oromia, and 11% in Addis Ababa.ConclusionThe findings of the meta-analysis indicating a high prevalence of fear of childbirth among pregnant mothers in Ethiopia and identifying associated risk factors highlight the importance of addressing this issue within the healthcare system. Integrating prevention-based services for mothers with childbirth fears into the antenatal care model could be a valuable approach to support women and mitigate the impact of fear on their reproductive experiences.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/#myprospero, identifier [CRD42023411103].
Senescence-related genes and proteins in the development of Alzheimer’s disease: evidence from transcriptomic and Mendelian randomization analysis
Authors: Liu Ying , Chen Jiao 
Published: August 2024

PurposeAlzheimer’s disease (AD) is a common neurodegenerative disease, which can lead to cognitive impairment and dementia. Since AD is tightly associated with aging and cellular senescence, objective of this study was to investigate the association between senescence-related genes and proteins (SRGs and SRPs) and the development of AD.DesignThe whole study was based on transcriptomic analysis of control and AD brain tissues and Mendelian randomization (MR) analysis.MethodsFor transcriptomic analysis, GSE5281 dataset from GEO database contains the transcriptomic data of human brain tissues (n = 161) from control group and AD patients. The expression of SRGs in control and AD brain tissues were compared by Student’s t test. For MR analysis, the instrumental single-nucleotide polymorphisms (SNPs) associated with 110 SRPs were filtered and selected from a large genome-wide association study (GWAS) for plasma proteome. The causality between plasma levels of SRPs and AD was explored using GWAS data of AD from Lambert et al. (17,008 cases and 37,154 controls) and further validated by using data from FinnGen consortium (6,489 patients and 170,489 controls). MR estimate was performed using the inverse-variance weighted (IVW) method and the heterogeneity and pleiotropy of results were tested.ResultsTranscriptomic analysis identified 36 up-regulated (including PLAUR) and 8 down-regulated SRGs in AD brain tissues. In addition, the MR results at both discovery and validation stages supported the causality between plasma levels of PLAUR (IVW-p = 3.04E-2, odds ratio [OR] = 1.15), CD55 (IVW-p = 1.56E-3, OR = 0.86), and SERPINE2 (IVW-p = 2.74E-2, OR = 0.91) and the risk of AD.ConclusionOur findings identified that PLAUR, as an SRG, may take part in the development of AD and found that high plasma levels of PLAUR was associated with increased risk of AD, indicating that this gene was a risk factor for this disease and providing the rationale of existing drugs or new preventative and therapeutic strategies.
Fully-automated production of [68Ga]Ga-Trivehexin for clinical application and its biodistribution in healthy volunteers
Authors: Wang Binchen , Jiang Yaqun , Zhu Jiaxu , Wu Huiqin , Wu Jianyuan , Li Ling , Huang Jianying , Xiao Zhiwei , He Yong 
Published: August 2024

BackgroundThe αvβ6-integrin targeting trimeric ligand [68Ga]Ga-Trivehexin has emerged as a promising candidate for clinical application due to its clinical imaging potentials in various malignant cancers. Our objective was to develop a simplified and reproducible module-based automated synthesis protocol to expand its availability in clinical application.MethodsThe pH value and the precursor load of radiolabeling were explored using an iQS-TS fully-automated module. Radiochemical purity was evaluated by radio-HPLC and radio-TLC. The ethanol content, radionuclide purity and identity, bacterial endotoxins, sterility, and stability of the final product [68Ga]Ga-Trivehexin were all tested. Biodistribution of [68Ga]Ga-Trivehexin in healthy volunteers was also conducted.ResultsThe synthesis was explored and established using fully-automated module with outstanding radiochemical purity (&gt;99%). Considering molar activity and economic costs, a pH of 3.6 and precursor dose of 30 μg were determined to be optimal. All relevant quality control parameters were tested and met the requirement of European Pharmacopoeia. In vitro stability test and imaging in healthy volunteer indicated the practical significance in clinical routines.ConclusionsA fully-automated synthesis protocol for [68Ga]Ga-Trivehexin using the iQS-TS synthesis module was achieved and conformed to the clinical quality standards.Clinical trial registrationClinicalTrials.gov, NCT05835570. Registered 28 April 2023, https://www.clinicaltrials.gov/study/NCT05835570.
Optimization of culture conditions for endophytic bacteria in mangrove plants and isolation and identification of bacteriocin
Authors: Peng Jinju , Xie Xingpeng , Fan Tingli , Ma Haotian , Li Yang , Luo Shuaishuai , Yu Mengbo , Ding Yuexia , Ma Yi 
Published: August 2024

Introduction: The antibacterial protein PAG14 was extracted from a metabolite of Bacillus G14 isolated from mangrove plants.Methods: In this study, Pseudomonas aeruginosa, Pasteurell multocide, Enterobacter aerogenes, and Enterococcus faecalis were used as indicator bacteria to screen endophytes that exhibited antibacterial activity. The endophyte culture conditions were optimized to enhance productivity. Subsequently, the culture supernatant was salted using ammonium sulfate, followed by purification using dextran gel chromatography and ion exchange column techniques. Finally, the structures of antibacterial proteins were identified using mass spectrometry.Results and Discussion: The optimal culture conditions for Bacillus G14 were 2% mannitol, 0.5% fish peptone, 0.05% KH2PO4 + 0.05% K2HPO4 + 0.025% MnSO4·H2O. The antibacterial substances exhibited stability within the temperature range of 30–40°C and pH range of 5.0–7.0, while displaying sensitivity toward enzymes. The antibacterial activity decreased as the duration of UV irradiation increased. The antibacterial protein PAG14, isolated from the culture broth of Bacillus G14 through purification using dextran gel and ion-exchange columns, was identified as a class III bacteriocin using LC-MS/MS, similar to Lysozyme C. These findings serve as a theoretical foundation for the investigation and application of bacteriocins in food products.
Selective inhibitor of sodium-calcium exchanger, SEA0400, affects NMDA receptor currents and abolishes their calcium-dependent block by tricyclic antidepressants
Authors: Boikov Sergei I. , Karelina Tatiana V. , Sibarov Dmitry A. , Antonov Sergei M. 
Published: August 2024

The open-channel block of N-methyl-D-aspartate receptors (NMDARs) and their calcium-dependent desensitization (CDD) represent conventional mechanisms of glutamatergic synapse regulation. In neurotrauma, neurodegeneration, and neuropathic pain the clinical benefits of cure with memantine, ketamine, Mg2+, and some tricyclic antidepressants are often attributed to NMDAR open-channel block, while possible involvement of NMDAR CDD in the therapy is not well established. Here the effects of selective high-affinity sodium-calcium exchanger (NCX) isoform 1 inhibitor, SEA0400, on NMDA-activated whole-cell currents and their block by amitriptyline, desipramine and clomipramine recorded by patch-clamp technique in cortical neurons of primary culture were studied. We demonstrated that in the presence of extracellular Ca2+, 50 nM SEA0400 caused a reversible decrease of the steady-state amplitude of NMDAR currents, whereas loading neurons with BAPTA or the removal of extracellular Ca2+ abolished the effect. The decrease did not exceed 30% of the amplitude and did not depend on membrane voltage. The external Mg2+ block and 50 nM SEA0400 inhibition of currents were additive, suggesting their independent modes of action. In the presence of Ca2+ SEA0400 speeded up the decay of NMDAR currents to the steady state determined by CDD. The measured IC50 value of 27 nM for SEA0400-induced inhibition coincides with that for NCX1. Presumably, SEA0400 effects are induced by an enhancement of NMDAR CDD through the inhibition of Ca2+ extrusion by NCX1. SEA0400, in addition, at nanomolar concentrations could interfere with Ca2+-dependent effect of tricyclic antidepressants. In the presence of 50 nM SEA0400, the IC50s for NMDAR inhibition by amitriptyline and desipramine increased by about 20 folds, as the Ca2+-dependent NMDAR inhibition disappeared. This observation highlights NCX1 involvement in amitriptyline and desipramine effects on NMDARs and unmasks competitive relationships between SEA0400 and these antidepressants. Neither amitriptyline nor desipramine could affect NCX3. The open-channel block of NMDARs by these substances was not affected by SEA0400. In agreement, SEA0400 did not change the IC50 for clomipramine, which acts as a pure NMDAR open-channel blocker. Thus, NCX seems to represent a promising molecular target to treat neurological disorders, because of the ability to modulate NMDARs by decreasing the open probability through the enhancement of their CDD.
The state of biosafety across China's CDC microbiology laboratories: insights from a nationwide survey (2021–2023)
Authors: Niu Peihua , Sun Zhenlu , Zhang Ruiqing , Zhao Yiming , Tian Fengyu , Cheng Ping , Zheng Hongmei , Guo Jianqiang , Zhang Meng , Ma Xuejun , Wang Ji 
Published: August 2024

BackgroundThe COVID-19 pandemic underscored the critical importance of biosafety in microbiology laboratories worldwide. In response, China has ramped up its efforts to enhance biosafety measures within its Centers for Disease Control and Prevention (CDC) laboratories. This study provides the first comprehensive assessment of biosafety practices across provincial, city, and county levels of CDC microbiology laboratories in China.MethodsWe conducted a nationwide cross-sectional survey from 2021 to 2023, targeting staff from microbiology laboratories within CDCs at all administrative levels in China. Stratified sampling was employed to select respondents, ensuring a representative mix across different CDC hierarchies, job titles, and academic qualifications. The survey encompassed questions on biosafety training, the presence of BSL-2 and BSL-3 laboratories, adherence to general biosafety guidelines, and management practices regarding specimens, reagents, and consumables. Statistical analysis was performed to identify significant differences in biosafety practices among different CDC levels.ResultsA total of 990 valid responses were received, highlighting a nearly universal presence (98.69%) of BSL-2 laboratories and a significant yet varied presence of BSL-3 laboratories across the CDC network. The survey revealed high levels of biosafety training (98.69%) and adherence to biosafety protocols. However, challenges remain in the consistent application of certain safety practices, especially at lower administrative levels. Notable differences in the management of specimens, reagents, and consumables point to areas for improvement in ensuring biosecurity.ConclusionOur findings indicate a robust foundation of biosafety practices within CDC microbiology laboratories in China, reflecting significant advancements in the wake of the Biosecurity Law's implementation. Nevertheless, the variability in adherence to specific protocols underscores the need for ongoing training, resources allocation, and policy refinement to enhance biosafety standards uniformly across all levels. This study's insights are crucial for guiding future improvements in laboratory biosafety, not just in China but potentially in other countries enhancing their public health infrastructures.
Neurosurgery training camp for medical student: experience of the Turkish neurosugery academy and Bursa Uludag University
Authors: Unal H. Setenay , Aydin Mevlut Okan , Bilgic Esma , Eser Pınar , Alper Zuleyha , Taskapılıoğlu M. Ozgur , İlker Kafa M. , Kocaeli Hasan , Dogan Seref , Yılmazlar Selcuk , Bekar Ahmet , Sekerci Zeki , Aksoy Kaya 
Published: August 2024

IntroductionTo highlight the importance of hands-on experiences and mentorship in shaping the future workforce of specialized medical professionals via a Neurosurgery Training Camp.MethodsResponses of the questionnaire regarding the Neurosurgery Training Camp organized by Bursa Uludag University's Faculty of Medicine and the Turkish Neurosurgery Academy were reviewed retrospectively. A one-day program was organized to introduce neurosurgery to medical students. During the camp, the students participated in interactive presentations delivered by faculty members, had lunch together, became acquainted with neurosurgical tools and technologies, and performed interventions. With pre and postworkshop questionnaire, student's expectations and thoughts about camp was evaluated.ResultsForty-one students from 10 medical schools, spanning every year of study, attended the camp. Approximately 39% of the attendees (n = 16) were women and 61% (n = 25) were men. The post-workshop survey results demonstrated that 73% of the students (n = 30) were inclined to pursue a career in neurosurgery after the camp, 21.9% (n = 9) remained undecided, and 4.8% (n = 2) chose not to pursue neurosurgery. Feedback from the post-workshop questionnaire highlighted that all students perceived the camp as beneficial in providing insights into their future careers and aiding in making a decision regarding their career paths.DiscussionThe neurosurgical training camp effectively inspired and educated medical students about the discipline of neurosurgery. Furthermore, the camp effectively altered the career aspirations and perceptions of neurosurgery among the participating students.
Prophylactic Peri-Nephric Drain Placement in Renal Transplant Surgery: A Systematic Review and Meta-Analysis
Authors: Lakha Adil S. , Ahmed Shahzaib , Hunter James , O’Callaghan John 
Published: August 2024

Renal transplantation is common worldwide, with &gt;25,000 procedures performed in 2022. Usage of prophylactic perinephric drains is variable in renal transplantation; drains are associated with risks, and there is a lack of consensus regarding benefit of routine drain placement in these patients. This meta-analysis assessed whether prophylactic drainage reduced need for reintervention postoperatively. This systematic review and meta-analysis was carried out using the Preferred Reporting Items in Systematic Reviews and Meta-Analysis, and prospectively registered on PROSPERO. Summary statistics for outcomes of interest underwent meta-analyses to a confidence interval (CI) of 95% and are presented as Forest Plots for Odds Ratio (OR). A systematic literature search in June 2023 revealed 1,540 unique articles across four databases. Of these, four retrospective cohort studies were selected. Meta-analysis of three studies showed no significant reduction in reintervention rate with pre-emptive drain placement, OR = 0.59 (95% CI: 0.16–2.23), p = 0.44. Meta-analysis did not show a significant reduction in perinephric collections with prophylactic drain insertion OR = 0.55 (95% CI: 0.13–2.37), p = 0.42. Finally, there is not good evidence that drain placement reduces superficial wound complications or improves 12-month graft survival. Further work is needed, including well-designed, prospective studies to assess the risks and benefits of drain placement in these patients.Systematic Review Registration: https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42023422685, Identifier PROSPERO CRD42021255795.
Functional Characterization of PagMYB148 in Salt Tolerance Response and Gene Expression Analysis under Abiotic Stress Conditions in Hybrid Poplar
Authors: Park Su Jin , Jang Hyun-A , Lee Hyoshin , Choi Hyunmo 
Published: August 2024

MYB transcription factors containing an R2R3 DNA-binding domain are involved in the regulation of various physiological processes, such as responses to abiotic stresses, including salt stress. In poplar, various MYB transcription factors regulate stress responses. In this study, we aimed to identify the correlation between the transcription factor MYB148 and salt stress responses in the hybrid poplar (Populus alba × P. glandulosa). We induced a mutation in the MYB-binding domain of PagMYB148 using CRISPR/Cas9-mediated editing of the PagMYB148 gene, which induced a more suppressed expression of PagMYB148 than that in the wild-type. Although salt and drought treatments enhance the expression of PagMYB148, the pagmyb148-transgenic plants exhibited more sensitive phenotypes than the wild-type plants under salt stress. After exposure to salinity stress, the chlorophyll content was lower in pagmyb148-transgenic plants than in wild-type plants, whereas the mutation increased ion leakage from cells. Additionally, the expression of genes involved in the salt stress response was higher in pagmyb148-transgenic plants than in the wild-type. After salt treatment, pagmyb148-transgenic plants exhibited an increased level of H2O2 and reduced activity of antioxidant enzymes. In summary, the MYB148 transcription factor is involved in the regulation of salt stress resistance in hybrid poplar trees. This report contributes to providing a basis for further investigating the molecular mechanisms of the poplar PagMYB148 transcription factor under abiotic stress.
Estudo da anemia em pacientes cirúrgicos: correlação clínica, prevalência e impacto na morbimortalidade cirúrgica
Authors: Lopes Otávio Romanini , Lopes Fernanda Romanini , Martins Laura Bisinotto , Bisinotto Flora Margarida Barra 
Published: August 2024

A anemia no paciente cirúrgico é um problema comum e apresenta uma prevalência elevada. Os pacientes com anemia pré-operatória apresentam significativamente maior taxa de morbimortalidade e maior probabilidade de serem transfundidos. Este estudo teve como objetivo revisar e analisar as características e consequências da anemia pré-operatória, investigando sua prevalência na população cirúrgica, sua correlação com dados clínicos e sua relação com o SUS e a rede suplementar de saúde. Foram analisados de forma retrospectiva 250 prontuários de pacientes do SUS atendidos no Hospital de Clínicas da UFTM e 250 pacientes da RSS atendidos em um hospital coparticipante, no período de janeiro de 2018 a abril de 2022. A presença de anemia foi definida segundo a Organização Mundial de Saúde como uma concentração de hemoglobina inferior a 13 g.dl-1 para homens e 12g.dl-1 para mulheres. A prevalência de anemia na população total do estudo foi de 13,8%, sendo 9,0% nos pacientes do SUS e 4,8% na RSS . Os pacientes do SUS com anemia apresentaram IMC significativamente menor em comparação com a RSS. A prevalência de anemia foi maior nos pacientes classificados segundo a Sociedade Americana de Anestesiologistas (ASA) como 3. Não houve diferença nos outros parâmetros avaliados. Este estudo confirmou que a anemia é uma condição comum nos pacientes cirúrgicos, principalmente nos pacientes do SUS. A classificação da ASA é um parâmetro importante para triagem. O diagnóstico de anemia no pré-operatório é importante pois permite prosseguir com a investigação e estabelecer um tratamento apropriado antes da cirurgia.
Pengaruh Fintech Peer to Peer Lending dan Fintech Payment Terhadap Kinerja UMKM DiMediasi E-Commerce Pasca Covid-19
Authors: Hidayatullah Aditya 
Published: August 2024

Pelaku UMKM di Kota Palembang saat ini masih mengalami kesulitan dalam mendapatkan pembiayaan atau permodalan. Tujuan dari penelitian ini untuk menguji pengaruh fintech peer to peer lending dan fintech payment terhadap Kinerja UMKM dimediasi e-commerce pasca Covid 19. Dengan adanya layanan fintech peer-to-peer lending dan fintech payment pelaku UMKM dapat memahami penggunaan layanan tersebut dan mampu meningkatkan kinerja usaha. UMKM subsektor kuliner dipilih sebagai objek pada penelitia ini. Sampel yang digunakan menggunakan teknik non-probability sampling dari 100 responden pelaku UMKM yang tersebar di lima Kecamatan Kota Palembang dengan karakteristik responden jenis kelamin, pendidikan terakhir, umur usaha dan omset penjualan perbulan. Metode penelitian ini menggunakan pendekatan kuantitatif dengan menyebar kuesioner kepada responden. Analisis data yang digunakan data primer dengan bantuan software SmartPLS 4 dengan evaluasi model pengukuran (outer model) dan model structural (inner model). Hasil penelitian menunjukkan bahwa fintech peer-to-peer lending berpengaruh negatif dan tidak signifikan terhadap kinerja UMKM, fintech payment berpengaruh positif dan signifikan terhadap kinerja UMKM, E-commerce berpengaruh positif dan signifikan terhadap kinerja UMKM, e-commerce sebagai variabel intervening memediasi secara positif dan signifikan antara fintech peer-to-peer lending terhadap kinerja UMKM, dan e-commerce sebagai variabel intervening memediasi secara positif dan signifikan antara Fintech Payment terhadap kinerja UMKM pasca Covid 19.
DESCRIPTION OF THE POTENTIAL OF SOME DIVE SPOTS ON LEMBEH BITUNG ISLAND
Authors: Pongtuluran Alma Karangan , Rompas Margresye Diana 
Published: August 2024

Pariwisata di Indonesia memiliki aset yang kompleks untuk dikelola lebih baik, salah satunya adalah wisata bahari. Banyak wilayah di Indonesia memiliki potensi wisata yang menarik sehingga bisa dikembangkan menjadi obyek wisata bahari. Pariwisata bahari (marine tourism) meliputi hal-hal yang terkait dengan kegiatan wisata, kesenangan dan rekreasi yang dilakukan di wilayah pesisir dan perairan laut.
Salah satu daerah di Indonesia yang memiliki potensi besar dalam wisata bahari ini adalah Sulawesi Utara. Sulawesi Utara mempunyai beberapa destinasi wisata yang banyak diminati oleh wisatawan baik dari asing maupun lokal.. Potensi pariwisata bahari di Sulawesi Utara yang cukup menjanjikan untuk dikembangkan selain Pulau Bunaken di Kota Manado berada di Kota Bitung tepatnya di Pulau Lembeh.
Pulau Lembeh adalah salah satu pilihan destinasi wisata yang dicari oleh para pecinta diving dan snorkling karena keindahan wisata bawah lautnya. Pesona Pulau Lembeh inilah yang dijadikan tujuan para wisatawan asing maupun lokal untuk sekedar bersantai atau melakukan diving dengan keindahan spot lautnya. Keindahan alam lautnya mampu menjadi daya tarik dari wisatawan asing maupun lokal untuk datang dan menikmatinya.