bluuebunny/crossref_metadata_2025_split huggingface dataset:
Violencia en el noviazgo y salud mental en estudiantes universitarios mexicanos
Authors: Lazarevich Irina , Irigoyen Camacho María E , Sokolova Anna V , Delgadillo Gutiérrez Hector J 
Published: August 2013

Antecedentes: En las últimas dos décadas, ha aumentado el interés por la promoción de la salud en el sector educativo superior, a través del programa “Universidades Saludables” promovido por la Organización Mundial de la Salud (OMS). Es importante reconocer las necesidades relacionadas con la salud de los integrantes de la comunidad universitaria, así como reflexionar sobre las acciones dirigidas a cubrirlas. Uno de los problemas prioritarios respecto a la salud mental de los jóvenes universitarios es la violencia en el noviazgo. Considerando lo anterior, los objetivos del presente estudio fueron: evaluar la violencia en el noviazgo, la sintomatología depresiva y la autoestima en estudiantes universitarios; estudiar la relación entre la violencia, la depresión y la autoestima; y promover la implementación de programas preventivos entre la comunidad universitaria. Métodos: Se realizó un estudio transversal y descriptivo con las respuestas de 729 estudiantes universitarios, aplicando el “Conflict in Adolescent Dating Relationship Inventory,” el Inventario de Depresión de Beck y el Inventario de Coopersmith para evaluar la autoestima. Resultados: Los resultados mostraron una elevada prevalencia de violencia en los jóvenes universitarios, particularmente verbal-emocional (75%). Las agresiones fueron de carácter bidireccional, tanto hombres como mujeres cometieron y sufrieron la violencia. La conducta violenta se asoció con la baja autoestima en las mujeres y con síntomas depresivos en ambos sexos. Conclusiones: Desde el enfoque del programa “Universidades Saludables,” es importante que las instituciones de educación superior sean promotoras de la salud mediante la creación y difusión del conocimiento, la formación de recursos humanos y la promoción de la organización comunitaria. Los resultados del estudio indican la necesidad de desarrollar programas educativos orientados a la prevención de la violencia, al manejo de emociones y a la resolución de conflictos.
A novel role for factor VIII and thrombin/PAR1 in regulating hematopoiesis and its interplay with the bone structure
Authors: Aronovich Anna , Nur Yaniv , Shezen Elias , Rosen Chava , Zlotnikov Klionsky Yael , Milman Irit , Yarimi Liran , Hagin David , Rechavi Gidi , Martinowitz Uriel , Nagasawa Takashi , Frenette Paul S. , Tchorsh-Yutsis Dalit , Reisner Yair 
Published: August 2013

Key PointsThe coagulation cascade via the factor VIII/thrombin/PAR1 axis regulates HSC maintenance. The coagulation cascade via factor VIII/thrombin/PAR1 axis regulates a reciprocal interplay between HSCs and the dynamic bone structure.
Human VKORC1 mutations cause variable degrees of 4-hydroxycoumarin resistance and affect putative warfarin binding interfaces
Authors: Czogalla Katrin J. , Biswas Arijit , Wendeln Ann-Christin , Westhofen Philipp , Müller Clemens R. , Watzka Matthias , Oldenburg Johannes 
Published: August 2013

Key PointsIn vitro analysis of VKORC1 mutations perfectly reflects patients’ warfarin resistance phenotypes. In silico docking of warfarin on a VKORC1 model reveals a putative docking site in agreement with the locations of OACR-associated mutations.
RNA-Seq effectively monitors gene expression in Eutrema salsugineum plants growing in an extreme natural habitat and in controlled growth cabinet conditions
Authors: Champigny Marc J , Sung Wilson WL , Catana Vasile , Salwan Rupa , Summers Peter S , Dudley Susan A , Provart Nicholas J , Cameron Robin K , Golding G Brian , Weretilnyk Elizabeth A 
Published: August 2013

AbstractBackgroundThe investigation of extremophile plant species growing in their natural environment offers certain advantages, chiefly that plants adapted to severe habitats have a repertoire of stress tolerance genes that are regulated to maximize plant performance under physiologically challenging conditions. Accordingly, transcriptome sequencing offers a powerful approach to address questions concerning the influence of natural habitat on the physiology of an organism. We used RNA sequencing ofEutrema salsugineum,an extremophile relative ofArabidopsis thaliana, to investigate the extent to which genetic variation and controlled versus natural environments contribute to differences between transcript profiles.ResultsUsing 10 million cDNA reads, we compared transcriptomes from two naturalEutremaaccessions (originating from Yukon Territory, Canada and Shandong Province, China) grown under controlled conditions in cabinets and those from Yukon plants collected at a Yukon field site. We assessed the genetic heterogeneity between individuals using single-nucleotide polymorphisms (SNPs) and the expression patterns of 27,016 genes. Over 39,000 SNPs distinguish the Yukon from the Shandong accessions but only 4,475 SNPs differentiated transcriptomes of Yukon field plants from an inbred Yukon line. We found 2,989 genes that were differentially expressed between the three sample groups and multivariate statistical analyses showed that transcriptomes of individual plants from a Yukon field site were as reproducible as those from inbred plants grown under controlled conditions. Predicted functions based upon gene ontology classifications show that the transcriptomes of field plants were enriched by the differential expression of light- and stress-related genes, an observation consistent with the habitat where the plants were found.ConclusionOur expectation that comparative RNA-Seq analysis of transcriptomes from plants originating in natural habitats would be confounded by uncontrolled genetic and environmental factors was not borne out. Moreover, the transcriptome data shows little genetic variation between laboratory YukonEutremaplants and those found at a field site. Transcriptomes were reproducible and biological associations meaningful whether plants were grown in cabinets or found in the field. Thus RNA-Seq is a valuable approach to study native plants in natural environments and this technology can be exploited to discover new gene targets for improved crop performance under adverse conditions.
Molecular footprints of domestication and improvement in soybean revealed by whole genome re-sequencing
Authors: Li Ying-hui , Zhao Shan-cen , Ma Jian-xin , Li Dong , Yan Long , Li Jun , Qi Xiao-tian , Guo Xiao-sen , Zhang Le , He Wei-ming , Chang Ru-zhen , Liang Qin-si , Guo Yong , Ye Chen , Wang Xiao-bo , Tao Yong , Guan Rong-xia , Wang Jun-yi , Liu Yu-lin , Jin Long-guo , Zhang Xiu-qing , Liu Zhang-xiong , Zhang Li-juan , Chen Jie , Wang Ke-jing , Nielsen Rasmus , Li Rui-qiang , Chen Peng-yin , Li Wen-bin , Reif Jochen C , Purugganan Michael , Wang Jian , Zhang Meng-chen , Wang Jun , Qiu Li-juan 
Published: August 2013

Abstract

Background
Artificial selection played an important role in the origin of modern Glycine max cultivars from the wild soybean Glycine soja. To elucidate the consequences of artificial selection accompanying the domestication and modern improvement of soybean, 25 new and 30 published whole-genome re-sequencing accessions, which represent wild, domesticated landrace, and Chinese elite soybean populations were analyzed.

Results
A total of 5,102,244 single nucleotide polymorphisms (SNPs) and 707,969 insertion/deletions were identified. Among the SNPs detected, 25.5% were not described previously. We found that artificial selection during domestication led to more pronounced reduction in the genetic diversity of soybean than the switch from landraces to elite cultivars. Only a small proportion (2.99%) of the whole genomic regions appear to be affected by artificial selection for preferred agricultural traits. The selection regions were not distributed randomly or uniformly throughout the genome. Instead, clusters of selection hotspots in certain genomic regions were observed. Moreover, a set of candidate genes (4.38% of the total annotated genes) significantly affected by selection underlying soybean domestication and genetic improvement were identified.

Conclusions
Given the uniqueness of the soybean germplasm sequenced, this study drew a clear picture of human-mediated evolution of the soybean genomes. The genomic resources and information provided by this study would also facilitate the discovery of genes/loci underlying agronomically important traits.
Genetic variability of Mycobacterium tuberculosiscomplex in patients with no known risk factors for MDR-TB in the North-eastern part of Lima, Peru
Authors: Barletta Francesca , Otero Larissa , Collantes Jimena , Asto Belisa , de Jong Bouke C , Seas Carlos , Rigouts Leen 
Published: August 2013

Abstract

Background
The aim of this study was to investigate the genetic diversity among Mycobacterium tuberculosis complex circulating in patients with no known risk factors for multi-drug resistant (MDR) tuberculosis (TB) living in a high MDR burden area and analyze the relationship between genotypes, primary drug resistance and age.

Methods
Samples were collected during January-July 2009. Isolates were tested for drug susceptibility to first-line drugs and were genotyped by spoligotyping and the 15-loci Mycobacterial Interspersed Repetitive Unit (MIRU15).

Results
Among the 199 isolates analyzed, 169 (84.9%) were identified in the SpolDB4.0 and 30 (15.1%) could not be matched to any lineage. The most prevalent lineage was Haarlem (29.6%), followed by T (15.6%), Beijing (14.1%), Latin American Mediterranean (12.6%) and U (8.5%). A few isolates belonged to the X and S clades (4.5%). Spoligotype analysis identified clustering among 148 of 169 isolates, whereas with MIRU15 all isolates were unique. Out of 197 strains; 31.5% were resistant to at least one drug, 7.5% were MDR and 22.3% showed any resistance to isoniazid.

Conclusion
In contrast with other Latin-American countries where LAM lineage is the most predominant, we found the spoligotype 50 from the Haarlem lineage as the most common. None of the prevailing lineages showed a significant association with age or resistance to isoniazid and/or rifampicin.
Protection of the heart by treatment with a divalent-copper-selective chelator reveals a novel mechanism underlying cardiomyopathy in diabetic rats
Authors: Zhang Lin , Ward Marie-Louise , Phillips Anthony RJ , Zhang Shaoping , Kennedy John , Barry Bernard , Cannell Mark B , Cooper Garth JS 
Published: August 2013

AbstractBackgroundIntracellular calcium (Ca2+) coordinates the cardiac contraction cycle and is dysregulated in diabetic cardiomyopathy. Treatment with triethylenetetramine (TETA), a divalent-copper-selective chelator, improves cardiac structure and function in patients and rats with diabetic cardiomyopathy, but the molecular basis of this action is uncertain. Here, we used TETA to probe potential linkages between left-ventricular (LV) copper and Ca2+homeostasis, and cardiac function and structure in diabetic cardiomyopathy.MethodsWe treated streptozotocin-diabetic rats with a TETA-dosage known to ameliorate LV hypertrophy in patients with diabetic cardiomyopathy. Drug treatment was begun either one (preventative protocol) or eight (restorative protocol) weeks after diabetes induction and continued thereafter for seven or eight weeks, respectively. Total copper content of the LV wall was determined, and simultaneous measurements of intracellular calcium concentrations and isometric contraction were made in LV trabeculae isolated from control, diabetic and TETA-treated diabetic rats.ResultsTotal myocardial copper levels became deficient in untreated diabetes but were normalized by TETA-treatment. Cardiac contractility was markedly depressed by diabetes but TETA prevented this effect. Neither diabetes nor TETA exerted significant effects on peak or resting [Ca2+]i. However, diabetic rats showed extensive cardiac remodelling and decreased myofibrillar calcium sensitivity, consistent with observed increases in phosphorylation of troponin I, whereas these changes were all prevented by TETA.ConclusionsDiabetes causes cardiomyopathy through a copper-mediated mechanism that incorporates myocardial copper deficiency, whereas TETA treatment prevents this response and maintains the integrity of cardiac structure and myofibrillar calcium sensitivity. Altered calcium homeostasis may not be the primary defect in diabetic cardiomyopathy. Rather, a newly-described copper-mediated mechanism may cause this disease.
Additive relationship between serum fibroblast growth factor 21 level and coronary artery disease
Authors: Shen Yun , Ma Xiaojing , Zhou Jian , Pan Xiaoping , Hao Yaping , Zhou Mi , Lu Zhigang , Gao Meifang , Bao Yuqian , Jia Weiping 
Published: August 2013

AbstractBackgroundExpression and activity of the fibroblast growth factor (FGF) 21 hormone-like protein are associated with development of several metabolic disorders. This study was designed to investigate whether serum FGF21 level was also associated with the metabolic syndrome-related cardiovascular disease, atherosclerosis, and its clinical features in a Chinese cohort.MethodsTwo-hundred-and-fifty-three subjects visiting the Cardiology Department (Sixth People's Hospital affiliated to Shanghai JiaoTong University) were examined by coronary arteriography (to diagnose coronary artery disease (CAD)) and hepatic ultrasonography (to diagnose non-alcoholic fatty liver disease (NAFLD)). Serum FGF21 level was measured by enzyme-linked immunosorbent assay and analyzed for correlation to subject and clinical characteristics. The independent factors of CAD were determined by multivariate logistic regression analysis.ResultsSubjects with NAFLD showed significantly higher serum FGF21 than those without NAFLD (388.0 pg/mL (253.0-655.4) vs. 273.3 pg/mL (164.9-383.7),P < 0.01). Subjects with CAD showed significantly higher serum FGF21, regardless of NAFLD diagnosis (P < 0.05). Serum FGF21 level significantly elevated with the increasing number of metabolic disorders (Pfor trend < 0.01). After adjustment of age, sex, and BMI, FGF21 was positively correlated with total cholesterol (P < 0.05) and triglyceride (P < 0.01). FGF21 was identified as an independent factor of CAD (odds ratio = 2.984, 95% confidence interval: 1.014-8.786,P < 0.05).ConclusionsIncreased level of serum FGF21 is associated with NAFLD, metabolic disorders and CAD.
Sildenafil ameliorates biomarkers of genotoxicity in an experimental model of spontaneous atherosclerosis
Authors: Rodrigues Bianca P. , Campagnaro Bianca P. , Balarini Camille M. , Pereira Thiago M. C. , Meyrelles Silvana S. , Vasquez Elisardo C. 
Published: August 2013

AbstractBackgroundIt is well known that enhanced production of reactive oxygen species (ROS) leads to oxidative stress observed in atherosclerosis and that ROS can also cause damage in cellular macromolecules, including DNA. Considering previous report that sildenafil, an inhibitor of phosphodiesterase 5 (PDE5), has antioxidant effects, in the present study we evaluated the effect of this drug on genotoxicity of blood mononuclear cells (MNC) and liver cells from atherosclerotic apolipoprotein E knockout mice (apoE-/-).MethodsROS production in MNC was evaluated by flow cytometry with the fluorescent dye dihydroethidium (DHE), a method that has been used to quantify the production of superoxide anion, and DNA damage was evaluated in both MNC and liver cells using the alkaline comet assay. Sildenafil-administered apoE-/-mice were compared with strain-matched mice administered with vehicle and with C57BL/6 wild-type (WT) mice.ResultsMNC from apoE-/-vehicle exhibited a 2-fold increase in production of superoxide anion in comparison with WT. In contrast, sildenafil-administered apoE-/-mice showed superoxide anion levels similar to those observed in WT mice. Similarly, MNC and liver cells from apoE-/-vehicle mice showed a 4-fold and 2-fold augmented DNA fragmentation compared with WT, respectively, and sildenafil-administered apoE-/-mice exhibited minimal DNA damage in those cells similar to WT mice.ConclusionsApoE-/-mice chronically administered with sildenafil exhibited reduced levels of superoxide anion in MNC and less DNA fragmentation in MNC and liver cells, which are biomarkers of genotoxicity. Therefore, sildenafil may offer a new perspective to the use of PDE5 inhibitors to protect against DNA damage, in cells involved in the inflammatory and dyslipidemic processes that accompany atherosclerosis.
InAs/GaAs quantum dots with wide-range tunable densities by simply varying V/III ratio using metal-organic chemical vapor deposition
Authors: Li Senlin , Chen Qingqing , Sun Shichuang , Li Yulian , Zhu Qiangzhong , Li Juntao , Wang Xuehua , Han Junbo , Zhang Junpei , Chen Changqing , Fang Yanyan 
Published: August 2013

Abstract
The complicated behaviors of InAs/GaAs quantum dots with increasing V/III ratio associated with several competing mechanisms have been described. The results demonstrate that the densities of InAs quantum dots can be tuned in a wide range from 105 to 1010 cm−2 by simply manipulating V/III ratio via metal-organic chemical vapor deposition. These results are mainly ascribed to the changes of coverage and In adatom migration length due to the increasing V/III ratio.
Cocaine-associated hemoperitoneum following atraumatic splenic rupture: a case report and literature review
Authors: Azar Faris , Brownson Elisha , Dechert Tracey 
Published: August 2013

Abstract

Introduction
Splenic hematoma or rupture of the spleen is rare in the absence of trauma. This case report with a brief review of the literature is intended to raise awareness of splenic bleeding as an etiology of abdominal pain; it highlights the importance of a detailed social history.

Presentation of case
This report of an otherwise healthy 42-year old man details hemoperitoneum with splenic rupture as a cause for hemorrhage following cocaine use. The patient was managed non-operatively in the surgical intensive care unit. He did not require transfusion and was discharged home on hospital day four with close follow-up.

Discussion
While splenic pathology associated with cocaine use has been described, this case illustrates a novel report of cocaine-associated splenic hemorrhage. A plausible mechanism is transient vasospasm with subsequent bleeding into the infarcted area.

Conclusion
Although uncommon, atraumatic splenic rupture should be recognized early because it is potentially fatal. This case is the first to describe hemoperitoneum of splenic etiology following cocaine use.
A newly isolated Bacillus licheniformisstrain thermophilically produces 2,3-butanediol, a platform and fuel bio-chemical
Authors: Li Lixiang , Zhang Lijie , Li Kun , Wang Yu , Gao Chao , Han Binbin , Ma Cuiqing , Xu Ping 
Published: August 2013

Abstract

Background
2,3-Butanediol (2,3-BD), a platform and fuel bio-chemical, can be efficiently produced by Klebsiella pneumonia, K. oxytoca, and Serratia marcescens. However, these strains are opportunistic pathogens and not favorable for industrial application. Although some generally regarded as safe (GRAS) microorganisms have been isolated in recent years, there is still a demand for safe 2,3-BD producing strains with high productivity and yield under thermophilic fermentation.

Results

Bacillus licheniformis strain 10-1-A was newly isolated for 2,3-BD production. The optimum temperature and medium pH were 50°C and pH 7.0 for 2,3-BD production by strain 10-1-A. The medium composition was optimized through Plackett–Burman design and response surface methodology techniques. With a two-stage agitation speed control strategy, 115.7 g/L of 2,3-BD was obtained from glucose by fed-batch fermentation in a 5-L bioreactor with a high productivity (2.4 g/L·h) and yield (94% of its theoretical value). The 2,3-BD produced by strain 10-1-A comprises (2R,3R)-2,3-BD and meso-2,3-BD with a ratio of nearly 1:1. The bdh and gdh genes encoding meso-2,3-butanediol dehydrogenase (meso-BDH) and glycerol dehydrogenase (GDH) of strain 10-1-A were expressed in Escherichia coli and the proteins were purified. meso-2,3-BD and (2R,3R)-2,3-BD were transformed from racemic acetoin by meso-BDH and GDH with NADH, respectively.

Conclusions
Compared with the reported GRAS 2,3-BD producers, B. licheniformis 10-1-A could thermophilically produce 2,3-BD with a high concentration, productivity and yield. Thus, the newly isolated GRAS strain 10-1-A might be a promising strain for industrial production of 2,3-BD. Two key enzymes for meso-2,3-BD and (2R,3R)-2,3-BD production were purified and further studied, and this might be helpful to understand the mechanism for 2,3-BD stereoisomers forming in B. licheniformis.
Efficient production of l-lactic acid by an engineered Thermoanaerobacterium aotearoensewith broad substrate specificity
Authors: Yang Xiaofeng , Lai Zhicheng , Lai Chaofeng , Zhu Muzi , Li Shuang , Wang Jufang , Wang Xiaoning 
Published: August 2013

Abstract

Background
Efficient conversion of lignocellulosic biomass to optically pure lactic acid is a key challenge for the economical production of biodegradable poly-lactic acid. A recently isolated strain, Thermoanaerobacterium aotearoense SCUT27, is promising as an efficient lactic acid production bacterium from biomass due to its broad substrate specificity. Additionally, its strictly anaerobic and thermophilic characteristics suppress contamination from other microoragnisms. Herein, we report the significant improvements of concentration and yield in lactic acid production from various lignocellulosic derived sugars, achieved by the carbon flux redirection through homologous recombination in T. aotearoense SCUT27.

Results

T. aotearoense SCUT27 was engineered to block the acetic acid formation pathway to improve the lactic acid production. The genetic manipulation resulted in 1.8 and 2.1 fold increase of the lactic acid yield using 10 g/L of glucose or 10 g/L of xylose as substrate, respectively. The maximum l-lactic acid yield of 0.93 g/g glucose with an optical purity of 99.3% was obtained by the engineered strain, designated as LA1002, from 50 g/L of substrate, which is very close to the theoretical value (1.0 g/g of glucose). In particular, LA1002 produced lactic acid at an unprecedented concentration up to 3.20 g/L using 10 g/L xylan as the single substrate without any pretreatment after 48 h fermentation. The non-sterilized fermentative production of l-lactic acid was also carried out, achieving values of 44.89 g/L and 0.89 g/g mixed sugar for lactic acid concentration and yield, respectively.

Conclusions
Blocking acetic acid formation pathway in T. aotearoense SCUT27 increased l-lactic acid production and yield dramatically. To our best knowledge, this is the best performance of fermentation on lactic acid production using xylan as the sole carbon source, considering the final concentration, yield and fermentation time. In addition, it should be mentioned that the performance of non-sterilized simultaneous fermentation from glucose and xylose was very close to that of normal sterilized cultivation. All these results used the mutant strain, LA1002, indicated that it is a new promising candidate for the effective production of optically pure l-lactic acid from lignocellulosic biomass.