bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract C05: Analysis of oxidative modified proteins in serous subtype of ovarian carcinoma
Authors: Mehrabi Sharifeh , Cohen Shakeria , Partridge Edward E. , Aikhionbare Felix O. 
Published: May 2015

Abstract
Serous subtype of ovarian cancer accounts for approximately 60% to 80% of ovarian cancer cases and is by far the most aggressive histology. Fewer than 25% of the serous ovarian cancer cases are detected at an early stage (stages I and II), a statistic which reflects grimly on the low survival rates, especially among African-American women. Elevated levels of reactive oxygen species (ROS) are one of many of known and suspected risk factors associated with ovarian cancer. Oxidative stress, which results from an imbalance between ROS and antioxidant capacities, can cause a wide range of direct or indirect DNA damage. There are extensive DNA repair systems that can correct DNA damage caused by ROS before cell replication and mutation fixation. Given that serous cancer subtype is considered to have originated from the fallopian epithelium mucosa that was exposed to immense physiological changes, driven by cyclically altering hormone levels as a result of ovulation. The influence of ovulation is a cause of increased oxidative stress. In this study, we applied spectrophotometric DNPH assay, two dimension gel electrophoresis (2-D SDS-PAGE), and Oxidized protein western blot analysis to identify and evaluate the level of oxidative modified protein, using derivatisation of protein carbonyl group with 2,4-dinitrophenylhyrdozine(DNPH) assay in the obtained 155 snap-freeze primary serous epithelial ovarian tissues including, normal, normal surrounding, cystoadenoma, borderline and carcinoma with age range of 65±12, (55% Caucasian 45% Africa American). Our results showed that the index of carbonyl protein /total protein increased by onset and progression of the serous ovarian malignancy. The elevation of oxidized protein among African American was 40 % higher than Caucasian in advanced stages of disease. SDS-PAGE profiles and western blot analysis of oxidized proteins showed a distinct protein pattern among cancerous and non-cancerous tissues. Our study suggests that level of oxidative stress, may involve the selective modification of some enzymes and structural proteins and play a role in etiological differences that may exist between stages of benign and invasive epithelial ovarian tumors.
This study was funded in part as a result of the grants from NIH-CHDRD CA150317 and NCI CA150039.
Citation Format: Sharifeh Mehrabi, Shakeria Cohen, Edward E. Partridge, Felix O. Aikhionbare. Analysis of oxidative modified proteins in serous subtype of ovarian carcinoma. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C05. doi:10.1158/1538-7755.DISP13-C05
Abstract C09: Planning for the future: Reaching an inner city Mexican American population
Authors: Strom Sara S. , Yamamura Yuko 
Published: May 2015

Abstract
Hispanics comprise 17% of the overall US population and 40% of the population in Texas. Approximately 90% of Hispanic Texans are of Mexican descent. This population presents unique challenges in access to health care and utilization of preventive screening. Although the internet has been rapidly integrated into education and outreach strategies for cancer prevention, its potential impact in reducing health disparities among Hispanics has not been evaluated. The few surveys conducted to date among Hispanics have focused on only those individuals who use the internet and were primarily done for marketing purposes. This study was conducted to identify differences between individuals who use internet compared to non-users in a large cohort of Mexican Americans residing in inner city neighborhoods (Houston, TX). Given that technology has evolved so quickly over the past decade, we limited our analyses to 1313 individuals enrolled in the past 2 years. In our population, most of the participants (58%, N=756) reported that they do not use the internet.
Using multivariable logistic regression, we constructed the final model in a backwards, stepwise approach to determine factors associated with internet use. Compared to those who reported using the internet, non-users were significantly (P&lt;0.05) more likely to be male, older (mean age 51.4 vs. 41.3 years old), non-English speakers (71.4% vs. 42.0%), and not have private insurance (14% vs. 25%). Notably, 87% of participants that did not use the internet reported that they almost always spoke in Spanish; in contrast, 44% of internet users reported that they almost always spoke in English. Overall, individuals who do not use the internet were more likely to have traits associated with poorer health and higher risk for health disparity.
One of the most important findings from this study is that almost 60% of the Hispanics in this population do not use the internet; additionally, among individuals who use the internet, only 61% reported using any form of social media (Facebook, Google+, Twitter, myspace). These results suggest that the use of social media and internet would not be effective methods to reach this population and have an impact in reducing health disparities in an inner city minority population.
Citation Format: Sara S. Strom, Yuko Yamamura. Planning for the future: Reaching an inner city Mexican American population. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C09. doi:10.1158/1538-7755.DISP13-C09
Abstract C29: Occurrence of comorbidities among multi-ethnic breast cancer survivors
Authors: Ashing Kimlin T. 
Published: May 2015

Abstract
Purpose: Disease co-occurrence is not uncommon. Comorbidities are concurrent, etiologically independent chronic health conditions, unrelated to the disease under study. Cancer, unfortunately is no exception with 68-85% of adult cancer patients diagnosed with other chronic illnesses. Among cancer survivors comorbid conditions that can negatively influence health and survivorship outcomes. Ethnic minorities report more co-occurring illnesses and poorer survivorship outcomes. Thus, the purpose of this study was to document the occurrence of comorbidities among African-American and Latina (English language preferred (ELP) and Spanish language preferred (SLP)) breast cancer survivors (BCS).
Methods: In total, 88 African-American, 95 ELP Latina, and 137 SLP Latina BCS were recruited via case ascertainment from the California Cancer Registry and hospital registries. Participants completed a self-report questionnaire in which they provided demographic and cancer-related medial characteristics and completed a comorbidity checklist indicating their comorbid conditions.
Results: Almost 1 in 4 BCS endorsed at least one comorbid condition with arthritis (37%), high blood pressure (37%), psychological difficulties (29%), and diabetes (19%) being among the most commonly endorsed. The occurrence of comorbidities differed by ethnic and linguistic group. Specifically, SLP Latina BCS were more likely to report having diabetes (29%) and experiencing psychological difficulties (42%). Latina BCS were twice as likely to endorse osteoporosis and headaches compared to African-Americans. Hypertension (49%) and arthritis (48%) most commonly co-occurred in African-American BCS. Older age was correlated with arthritis, diabetes, glaucoma, high blood pressure, and osteoporosis.
Conclusions: The findings suggest that investigating the occurrence of comorbidities in ethnically diverse groups may shed some light in understanding cancer risk, outcomes and cancer health disparities. Having a better grasp of comorbid conditions in specific ethnic groups may aid in more appropriate early co-occurring chronic illness assessment and integration into cancer treatment regimens, and better follow-up care and management of the cancer and the comorbid condition(s). Integrated control and management of comorbidities has the potential to improve quality care for the whole person, and increase survival and decrease morbidity.
Citation Format: Kimlin T. Ashing. Occurrence of comorbidities among multi-ethnic breast cancer survivors. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C29. doi:10.1158/1538-7755.DISP13-C29
Abstract C32: Developing culturally appropriate interventions providing psychosocial and reproductive health support to young breast cancer survivors: Evaluation findings
Authors: Fairley Temeika L. , Buchanan Natasha , Johnson-Turbes Ashani , Schover Leslie R. , Moore Angela R. , Yancy Brandie , Schleuter Dara , Hall Mary-Ann K. , Hodges Kelly P. , Shoretz Rochelle 
Published: May 2015

Abstract
Introduction/Objectives: Research demonstrates that after a breast cancer diagnosis, young women, especially those of racial and ethnic groups, may experience a more negative impact on their quality of life, emotional functioning, and reproductive health than older women. For women under the age of 45, African American women have the highest breast cancer incidence of any racial/ethnic group, and there is an increased risk of hereditary breast cancer among Jews who carry BRCA mutations. In conjunction with Sisters Network Inc. and Sharsheret, The Centers for Disease Control and Prevention launched a formative evaluation to understand African American and Jewish young breast cancer survivors' (YBCS) psychosocial and reproductive health needs and preferred communication channels to obtain needed health information.
Methods: Sisters Network Inc. conducted four focus groups with African American YBCS. Sharsheret conducted four groups with Jewish YBCS. Thematic analysis identified major themes/patterns within and across the focus groups.
Results: Fertility and sexual health were major YBCS concerns. Participants reported barriers to obtaining psychosocial and reproductive health information, including sexual taboos and lack of provider sensitivity to address these needs. Participants said that breast cancer resources are not tailored to YBCS. Participants preferred to receive breast cancer information via multiple channels, including written materials, the Internet, telephone and smartphone applications.
Discussion/Implications: African American and Jewish women encounter similar barriers to obtaining psychosocial and reproductive health information. Both groups report difficulty in obtaining relevant health information and advice from their medical providers. Study findings may be used for developing of culturally-appropriate communication and interventions for YBCS that would help eliminate breast cancer health disparities in YBCS.
Citation Format: Temeika L. Fairley, Natasha Buchanan, Ashani Johnson-Turbes, Leslie R. Schover, Angela R. Moore, Brandie Yancy, Dara Schleuter, Mary-Ann K. Hall, Kelly P. Hodges, Rochelle Shoretz. Developing culturally appropriate interventions providing psychosocial and reproductive health support to young breast cancer survivors: Evaluation findings. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C32. doi:10.1158/1538-7755.DISP13-C32
Abstract C38: The decrease of the survivin protein expression induces chromosomal missegregation in colon cancer patients lymphocytes treated with oxaliplatin and vinflunine
Authors: Alotaibi Amal A. 
Published: May 2015

Abstract
Survivin is a novel member of the IAP family of proteins with a potential dual role in apoptosis inhibition and regulation of mitosis. In addition, survivin is implicated in the regulation of the mitotic spindle checkpoint and the promotion of angiogenesis, and chemoresistance. Survivin is up-regulated in almost all cancers, including colon cancer, but has low or no expression in most normal, differentiated adult tissues. Expression of survivin in cancer cells has been shown to promote tumorigenesis. Besides, the expression levels of survivin strongly correlate with the proliferative activity of the tumours indicating a possible role in cell cycle regulation and cancer progression.
However, the effect of a novel chemotherapy such as oxaliplatin and vinflunine on survivin expression has been not well characterized yet. Therefore, the effects of oxaliplatin and vinflunine treatment on survivin protein expression in lymphocytes from colon cancer patients against healthy volunteers were investigated. Treatment with either oxaliplatin or vinflunine significantly increased the formation of micronuclei with p &lt; 0.01 in dose-dependent manner by using a cytokinesis-block micronucleus (CBMN) assay. In addition, treatment with both compounds increased chromosomal missegregation (p&lt;0.01) as investigated by a combination of the CBMN assay with fluorescence in situ hybridisation (MN-FISH). Survivin protein expression in lymphocytes from colon cancer patients treated with oxaliplatin and vinflunine were evaluated by Western blot assay. The expression of survivin was significantly reduced up to 3-fold and 6-fold in survivin expression in all the concentrations of oxaliplatin and vinflunine tested respectively (p &lt; 0.001).
In conclusion, significant reduction in survivin protein expression was observed in lymphocytes from colon cancer patients exposed to oxaliplatin or vinflunine. Also evidence for mitotic catastrophe has been found for the first time as one plausible mechanism of oxaliplatin and vinflunine induced lymphocyte death. Oxaliplatin and vinflunine effectively induce DNA damage in vitro leading to aneuploidy in human lymphocytes observed in chromosome missegregation during M-phase. Future work will examine the actual impact of DNA damage to improve chemotherapy treatment or open a new approach in cancer therapy.
Citation Format: Amal A. Alotaibi. The decrease of the survivin protein expression induces chromosomal missegregation in colon cancer patients lymphocytes treated with oxaliplatin and vinflunine. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C38. doi:10.1158/1538-7755.DISP13-C38
Abstract C41: Palliative care consults in terminal cancer patients dying in a safety-net hospital: Are they underutilized?
Authors: Wiatrek Rebecca L. , Alawadi Zeinab I. , Lew Debbie F. , Zuo Melanie E. , Ferrer Jeanette G. , Ko Tien C. , Kao Lillian S. , Wray Curtis J. 
Published: May 2015

Abstract
Introduction: Terminal patients with limited resources are more likely to die in the hospital which may be due in part to lack of access to palliative care. We hypothesized that the majority of terminal cancer patients who were treated and who died in a safety-net hospital did not receive a palliative care consult.
Methods: A retrospective review of terminal cancer patients who were admitted and died at Lyndon Baines Johnson General Hospital, a safety-net hospital, from January through December 2012 was completed. Terminal cancer was defined as Stage IV or recurrent cancer with only palliative treatment options. Data was gathered on age, race/ethnicity, sex, length of stay (LOS) of final admission, underlying terminal cancer, insurance status, and receipt of a palliative consult. Univariate analysis was performed using chi-square and Kruskal-Wallis test.
Results: Seventy-four patients with terminal cancer died in the hospital. The majority of patients were minorities: African Americans (n=28, 38%), Hispanics (n=27, 36%), Caucasians (n=14, 19%), and Asian/other ethnicities (n=5, 7%). Two-thirds of patients (n=49, 66%) received palliative consults. There was no difference in race/ethnicity, gender, or age between patients who did and did not receive palliative consults. Patients who received a palliative consult were more likely to be uninsured (90% vs. 56%, p=0.003). There was no difference in median LOS (interquartile range, days) of the final admission between patients who did and did not receive a palliative consult (7, 3-16 versus 6, 2-15 days; p=0.47).
Conclusions: In a safety-net hospital, in-hospital deaths still occur among terminal cancer patients even when palliative care consults are received. Further studies are necessary to determine how palliative care can best address the needs of vulnerable patients in order to reduce in-hospital deaths and improve patient-centered outcomes.
Citation Format: Rebecca L. Wiatrek, Zeinab I. Alawadi, Debbie F. Lew, Melanie E. Zuo, Jeanette G. Ferrer, Tien C. Ko, Lillian S. Kao, Curtis J. Wray. Palliative care consults in terminal cancer patients dying in a safety-net hospital: Are they underutilized?. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C41. doi:10.1158/1538-7755.DISP13-C41
Abstract C64: Iroquois homeobox gene 3 is temporally regulated in response to vascular endothelial growth factor and modulates endothelial cell migration
Authors: Scarlett Kisha , Vaishnavi Pattabiraman , Anderson Leonard 
Published: May 2015

Abstract
Purpose: Differences in tumor-induced angiogenesis contributes to cancer-related health disparities. Understanding the transcriptional regulation of angiogenesis within endothelial cells (ECs) is essential to the development of anti-angiogenic therapies, and decreasing the health disparity gap in terms of cancer treatment. Here we report that the homeobox gene, irx3 is expressed in human microvascular endothelial cells (HMECs) and modulates their migration and ability to form networks during angiogenesis.
Methods: To test the effects of vascular endothelial growth factor (VEGF) on irx3 expression, HMECs were treated with VEGF for 0 to 48 hours followed by extraction of total RNA and protein. irx3 mRNA and protein expression were determined using qRT-PCR and western immunoblot respectively. Wound-healing and transwell migration assays were performed to investigate the role of irx3 on EC migration in response to VEGF. For migration experiments, HMECs were transduced with adenoviral vectors resulting in the loss-of (Ad.mirIrx3-GFP) or gain-of (Ad.Irx3-V5) irx3 function. We conducted a 672 transcription factor (TF) siRNA screen to identify transcriptional regulators of irx3 expression in response to VEGF. HMECs were reverse-transfected with siRNAs and treated with VEGF for 12 hours. Total RNA was extracted for RT-PCR to determine irx3 expression. Finally, we performed in vitro endothelial cell tubulogenesis assays using HMECs transduced with Ad.Irx3-V5, Ad.mirIrx3-GFP and their respective controls to study changes in endothelial tube formation as a result of differential irx3 expression.
Results: VEGF treatment increases irx3 expression at the mRNA and protein levels, with peak expression at 12 hours. HMECs transduced with Ad.mirIrx3-GFP exhibited decreased migration at 12 hours compared to HMECs transduced with Ad.Irx3-V5. Furthermore, when HMECs are transduced with Ad.Irx3-V5, they exhibit increased migration and invasiveness in transwell migration assays and form more complex networks during tube formation. The siRNA screen suggests that irx3 is regulated by HEY1, a known transcriptional regulator of angiogenesis.
Conclusion: Our results provide compelling evidence that the homeobox gene irx3 regulates EC migration, and is directly regulated by TFs known to govern transcriptional signaling during angiogenesis.
Citation Format: Kisha Scarlett, Pattabiraman Vaishnavi, Leonard Anderson. Iroquois homeobox gene 3 is temporally regulated in response to vascular endothelial growth factor and modulates endothelial cell migration. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C64. doi:10.1158/1538-7755.DISP13-C64
Abstract C65: Loss of imprinting of KCNK9 in African American women with triple-negative breast cancer
Authors: Desai Shraddha , Skaar David , Dietze Eric , Ambrose Adrian , Jirtle Randy , Seewaldt Victoria 
Published: May 2015

Abstract
Background: It has been long suspected that prenatal nutrition and environmental toxins play a role in promoting breast cancer. Genomic imprinting is an inherited form of parent-of-origin-specific epigenetic gene regulation that is dysregulated by poor prenatal nutrition and environmental toxins. Recent studies show that exposure to the environmental plastic bis-phenol A and vitamin D deficiencies promote loss of normal imprinting. African American (AA) women are known to have vitamin D deficiency at a significantly higher frequency than women of Northern European descent. Here we investigated a mechanism that may explain the higher rate of aggressive triple-negative breast cancers (TNBC) in AA women.
Methods and Results: KCNK9 encodes TASK3, a pH-regulated potassium channel membrane protein that is overexpressed in 40% of breast cancer. Our studies have identified a differentially methylated region (DMR) for KCNK9 and shown monoallelic expression of KCNK9 in breast tissue. Using patient samples, we show loss of imprinted expression in tumor cells, loss of KCNK9 regulatory methylation in 75% of TNBC samples, and loss of methylation in non-cancerous tissue from high-risk AA women. In live TNBC cells and non-cancerous mammary epithelial cells from high-risk women, loss of KCNK9 DMR methylation correlates with increased mitochondrial membrane potential.
Significance: This is the first identification of the KCNK9 DMR and demonstration that loss of methylation and overexpression of TASK3 increases mitochondrial membrane potential and promotes apoptosis resistance. These novel findings regarding KCNK9 loss of methylation and its disruption in a subset of tumors may lead to new approaches in diagnosis and targeted therapy of breast cancer.
Citation Format: Shraddha Desai, David Skaar, Eric Dietze, Adrian Ambrose, Randy Jirtle, Victoria Seewaldt. Loss of imprinting of KCNK9 in African American women with triple-negative breast cancer. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr C65. doi:10.1158/1538-7755.DISP13-C65