bluuebunny/crossref_metadata_2025_split huggingface dataset:
SRrp35 suppresses cell proliferation and malignancy in hepatocellular carcinoma
Authors: Li Yandong , Lin Yun , Li Qun , Gao Yong 
Published: January 2015

AimSerine/arginine‐rich (SR) proteins are known to be involved in alternative pre‐mRNA splicing, RNA metabolism and cancer development. SRrp35 is a member of this family that antagonizes classical SR proteins in the regulation of splicing. However, the function of SRRP35 in cancer remains unclear to date. This study aims to investigate SRrp35 function in hepatocellular carcinoma (HCC).MethodsSRrp35 expression was accessed by quantitative real‐time polymerase chain reaction in 32 pairs of clinical HCC samples and 12 kinds of HCC cell lines. The biological roles of Srrp35 were analyzed using cell viability, colony formation and soft agar growth assays in vitro and tumorigenicity in nude mice model.ResultsSRrp35 was frequently downregulated in 62.5% (20/32) of HCC specimens and most of HCC cell lines. Enhanced expression of SRrp35 significantly suppressed cell proliferation and colony formation in an anchor‐dependent and ‐independent manner, whereas knockdown of SRrp35 by RNA inference resulted in the opposite effects. In vivo, SRrp35 plays a suppressive role in developing tumor xenografts in line with the function in vitro.ConclusionOur findings suggest that SRrp35 suppresses cell proliferation and malignancy in HCC and could be a potential therapeutic target for this disease.
Consumer trust relations with payment cards and banks: an exploratory study
Authors: Koivunen Tuija , Tuorila Helena 
Published: January 2015

AbstractThe article focuses on the ways in which consumer complaints reflect the trust relationship between consumers, payment cards and banks from the consumers' point of view. The empirical data consist of consumer complaints fromFinland and are analysed with qualitative method. The data show that consumers use payment cards in various places for different purposes at home and abroad at least until something unexpected and problematic happens. According to the consumer complaints, in problem situations, the banks blame the consumers and categorically deny their responsibility. Negligence on the part of the consumers and questions concerning technology are the major reason for distrust concerning the reliability of payment cards. These findings provide bases for practical guidelines to increase trust in payment cards and, finally, implications for consumer education are discussed.
Collaboration between hospital and primary care nurses: a literature review
Authors: Lemetti T. , Stolt M. , Rickard N. , Suhonen R. 
Published: January 2015

BackgroundNurses play an important role in the treatment and care of adults in both hospital and primary health care working within complex and fragmented organizational systems. As the nature of health care changes and hospital and primary care sectors become more closely associated, nurses in both sectors have an obligation to increase their collaboration.AimsThis study aimed to increase the understanding of collaboration between nurses working with adults in hospital and primary health care, and to facilitate the future measurement of this collaboration.MethodsA literature review was undertaken in July and August 2013 using CINAHL and MEDLINE databases from the earliest to August 2013. The searches produced 4951 citations that were reduced to 22 articles for review using a four‐step inclusion strategy. Inductive content analysis was used to analyse the data.ResultsIt is suggested that collaboration is a process that contains (1) collaboration precursors: the opportunity to participate, knowledge and shared objectives; (2) elements of collaboration: competency, awareness and understanding of work roles and interaction; and (3) processes and outcomes: the events or behaviours that are the consequences of the collaboration between hospital and primary healthcare nurses.ConclusionsThe results indicate that collaboration between hospital and primary healthcare nurses is an important and integral part of the work of nurses and a process consisting of several predictable issues leading to useful care outcomes.Implications for nursing and health policyCurrent healthcare changes make it a requirement for hospital and primary healthcare nurses to collaborate when working with adults to continue to meet the needs of patients. The findings of this study can be used to improve collaboration in practice and to devise research to improve collaboration between hospital and primary healthcare nurses.
β‐elemene inhibited expression of DNA methyltransferase 1 through activation of ERK1/2 and AMPKα signalling pathways in human lung cancer cells: the role of Sp1
Authors: Zhao ShunYu , Wu Jingjing , Zheng Fang , Tang Qing , Yang LiJun , Li Liuning , Wu WanYin , Hann Swei Sunny 
Published: January 2015

Abstractβ‐elemene, a compound derived from Rhizoma zedoariae, is a promising new plant‐derived drug with broad‐spectrum anticancer activity. However, the underlying mechanism by which this agent inhibits human lung cancer cell growth has not been well elucidated. In this study, we showed that β‐elemene inhibits human non‐small cell lung carcinoma (NSCLC) cell growth, and increased phosphorylation of ERK1/2, Akt and AMPKα. Moreover, β‐elemene inhibited expression of DNA methyltransferase 1 (DNMT1), which was not observed in the presence of the specific inhibitors of ERK (PD98059) or AMPK (compound C). Overexpression of DNMT1 reversed the effect of β‐elemene on cell growth. Interestingly, metformin not only reversed the effect of β‐elemene on phosphorylation of Akt but also strengthened the β‐elemene‐reduced DNMT1. In addition, β‐elemene suppressed Sp1 protein expression, which was eliminated by either ERK1/2 or AMPK inhibitor. Conversely, overexpression of Sp1 antagonized the effect of β‐elemene on DNMT1 protein expression and cell growth. Taken together, our results show that β‐elemene inhibits NSCLC cell growth via ERK1/2‐ and AMPKα‐mediated inhibition of transcription factor Sp1, followed by reduction in DNMT1 protein expression. Metformin augments the effect of β‐elemene by blockade of Akt signalling and additively inhibition of DNMT1 protein expression. The reciprocal ERK1/2 and AMPKα signalling pathways contribute to the overall responses of β‐elemene. This study reveals a potential novel mechanism by which β‐elemene inhibits growth of NSCLC cells.
Advanced research on vasculogenic mimicry in cancer
Authors: Qiao Lili , Liang Ning , Zhang Jiandong , Xie Jian , Liu Fengjun , Xu Deguo , Yu Xinshuang , Tian Yuan 
Published: January 2015

AbstractVasculogenic mimicry (VM) is a brand‐new tumour vascular paradigm independent of angiogenesis that describes the specific capacity of aggressive cancer cells to form vessel‐like networks that provide adequate blood supply for tumour growth. A variety of molecule mechanisms and signal pathways participate in VM induction. Additionally, cancer stem cell and epithelial‐mesenchymal transitions are also shown to be implicated in VM formation. As a unique perfusion way, VM is associated with tumour invasion, metastasis and poor cancer patient prognosis. Due to VM's important effects on tumour progression, more VM‐related strategies are being utilized for anticancer treatment. Here, with regard to the above aspects, we make a review of advanced research on VM in cancer.
Evidence Aid approach to gap analysis and priority setting of questions for systematic reviews in disasters
Authors: Kayabu Bonnix 
Published: January 2015

AbstractThis article is based on a presentation at the Evidence Aid Symposium, on 20 September 2014, at Hyderabad, India. Ten years after the Indian Ocean Tsunami, Evidence Aid and it parters and other humanitarian stakeholders met to update about Evidence Aid work and discussed it future. The Evidence Aid approach to fill in the gap on the production and use of evidence in disater sector and other humanitarian health emergencies was widely discussed. Iterative approach to prioritise evidence reinforced Evidence Aid principle of independacy and a coordinated international orgasisation. The generation of 30 research questions during the prioritisation process contitute the first big step for Evidence Aid to become a one stop shop for the seach evidence on the effectiveness of interventions in disasters.
The quality analysis of literature retrievals of systematic reviews for traditional Chinese medicine
Authors: Chen Min , Xiao Yang , Liu Yuqi , Peng Yuanling , He Jia , Zhang Yonggang , Du Liang 
Published: January 2015

AbstractBackgroundThe literature retrievals are still the main problems which limit the quality of systematic review (SR) for traditional Chinese medicine (TCM), and there were still no comprehensive analyses for these problems. The aim of this study is to analyze these problems to guide future SRs for TCM.MethodsThe Cochrane Library (Issue 2, 2009) and the Chinese BioMedical Database (CBM, 1978‐ 2009.7.31) were comprehensively searched to identify the SRs of TCM. The native literature retrieval rate of the first author, the characteristics of the included databases, the number of the included database, the reporting of literature retrieval strategy, and the reporting of adjuvant search for both the SRs from the CBM and Cochrane Library were analyzed.ResultsA total of 341 SRs were included in our analyses, including 245 from the CBM and 96 from the CL. The Chinese‐authored SRs ranked the first in median retrieval rate (100%). The Cochrane SRs were significantly superior than the CBM SRs: more searched databases (media: 4 vs. 6, P < 0.001), more precision of retrieval time, more conducting gray literatures searches (47.92% vs. 20.41%) and handing searches (57.29% vs. 54.69%), and more reported retrieval strategies (66.67% vs. 8.16%).ConclusionsThere were significant problems in the literature retrieval of SRs for TCM. In future, when performing any SRs for TCM, sufficient and proper databases, clearly searching strategies and times, proper adjuvant retrievals should be reported. Cochrane SRs might be a better choice.
Critical appraisal of clinical practice guidelines for treating pancreatic cancer based on the global disease burden
Authors: Li Cui Cui , Wang Ying Qiang , Li You Ping , Li Xiang Lian 
Published: January 2015

AbstractObjectivesTo evaluate the quality of pancreatic cancer guidelines with evidence‐based methods based on the global burden of disease and to explore its status, distribution, characteristics of theme, and the difference of recommended therapies among various qualities of guidelines, so as to provide a reference for clinical decision.MethodsThe PubMed, The Cochrane Library (Issue 11, 2013), Chinese Biomedical Database, China National Knowledge Infrastructure, and VIP databases, as well as the website of National Guidelines Clearinghouse, Guidelines International Network, and National Institute for Clinical Excellence were systematically reviewed through November 2013 for pancreatic cancer guidelines. The Appraisal of Guidelines for Research and Evaluation (AGREE II) were applied to assess the methodological quality of the guidelines.ResultsA total of 14 relevant guidelines (including 5 evidence‐based ones) were identified, involving four continents (Asia, Europe, North America, and Oceania), seven counties, and four international organizations. There were only two domains, namely ‘scope and purpose’ and ‘clarity of presentations’, getting high average scores (more than 60%) among all 14 guidelines. The mean AGREE domain score in guidelines varies among different areas, and the quality of 5 evidence‐based guidelines was superior to that established by consensus. According to AGREE II, 11 guidelines were weakly recommended, while 3 were not recommended due to poor methodological quality. Their subjects of 14 guidelines covered six treatment categories, including chemotherapy, surgery, radiotherapy, support therapy, radiotherapy, and interventional therapy.ConclusionThe overall methodological quality of pancreatic cancer guidelines is suboptimal among different countries or regions. The qualities of evidence‐based guidelines are significantly superior to consensus. The chemotherapy, surgery, radiotherapy, and support therapy were the predominant choices by guidelines.
The methodological quality assessment tools for preclinical and clinical studies, systematic review and meta‐analysis, and clinical practice guideline: a systematic review
Authors: Zeng Xiantao , Zhang Yonggang , Kwong Joey S.W. , Zhang Chao , Li Sheng , Sun Feng , Niu Yuming , Du Liang 
Published: January 2015

AbstractObjectiveTo systematically review the methodological assessment tools for pre‐clinical and clinical studies, systematic review and meta‐analysis, and clinical practice guideline.MethodsWe searched PubMed, the Cochrane Handbook for Systematic Reviews of Interventions, Joanna Briggs Institute (JBI) Reviewers Manual, Centre for Reviews and Dissemination, Critical Appraisal Skills Programme (CASP), Scottish Intercollegiate Guidelines Network (SIGN), and the National Institute for Clinical Excellence (NICE) up to May 20th, 2014. Two authors selected studies and extracted data; quantitative analysis was performed to summarize the characteristics of included tools.ResultsWe included a total of 21 assessment tools for analysis. A number of tools were developed by academic organizations, and some were developed by only a small group of researchers. The JBI developed the highest number of methodological assessment tools, with CASP coming second. Tools for assessing the methodological quality of randomized controlled studies were most abundant. The Cochrane Collaboration's tool for assessing risk of bias is the best available tool for assessing RCTs. For cohort and case‐control studies, we recommend the use of the Newcastle‐Ottawa Scale. The Methodological Index for Non‐Randomized Studies (MINORS) is an excellent tool for assessing non‐randomized interventional studies, and the Agency for Healthcare Research and Quality (ARHQ) methodology checklist is applicable for cross‐sectional studies. For diagnostic accuracy test studies, the Quality Assessment of Diagnostic Accuracy Studies‐2 (QUADAS‐2) tool is recommended; the SYstematic Review Centre for Laboratory animal Experimentation (SYRCLE) risk of bias tool is available for assessing animal studies; Assessment of Multiple Systematic Reviews (AMSTAR) is a measurement tool for systematic reviews/meta‐analyses; an 18‐item tool has been developed for appraising case series studies, and the Appraisal of Guidelines, Research and Evaluation (AGREE)‐II instrument is widely used to evaluate clinical practice guidelines.ConclusionsWe have successfully identified a variety of methodological assessment tools for different types of study design. However, further efforts in the development of critical appraisal tools are warranted since there is currently a lack of such tools for other fields, e.g. genetic studies, and some existing tools (nested case‐control studies and case reports, for example) are in need of updating to be in line with current research practice and rigor. In addition, it is very important that all critical appraisal tools remain subjective and performance bias is effectively avoided.
Comparison of intraductal ultrasonography findings between primary sclerosing cholangitis and IgG4‐related sclerosing cholangitis
Authors: Naitoh Itaru , Nakazawa Takahiro , Hayashi Kazuki , Miyabe Katsuyuki , Shimizu Shuya , Kondo Hiromu , Nishi Yuji , Yoshida Michihiro , Umemura Shuichiro , Hori Yasuki , Kato Akihisa , Okumura Fumihiro , Sano Hitoshi , Ohara Hirotaka , Joh Takashi 
Published: January 2015

AbstractBackground and AimComparisons of intraductal ultrasonography (IDUS) findings between primary sclerosing cholangitis (PSC) and IgG4‐related sclerosing cholangitis (IgG4‐SC) have not been elucidated. We aimed to clarify the differences in transpapillary IDUS findings between PSC and IgG4‐SC.MethodsWe retrospectively compared transpapillary IDUS findings between 15 patients with PSC and 35 patients with IgG4‐SC between 2004 and 2014.ResultsIDUS findings of circular‐asymmetric wall thickness, irregular inner margin, diverticulum‐like outpouching, unclear outer margin, heterogeneous internal echo, and disappearance of three layers were significantly higher in PSC than in IgG4‐SC (P < 0.001). Irregular inner margin, diverticulum‐like outpouching, and disappearance of three layers were specific IDUS findings for PSC compared to IgG4‐SC. Diverticulum‐like outpouching on IDUS and endoscopic retrograde cholangiogram (ERC) was observed in 10 (67%) and five (33%) of 15 patients with PSC, respectively. However, based on IDUS and ERC, diverticulum‐like outpouching was not observed in any patient with IgG4‐SC. All five patients with diverticulum‐like outpouching on ERC had diverticulum‐like outpouching on IDUS, and five (50%) of 10 patients without diverticulum‐like outpouching on ERC had diverticulum‐like outpouching on IDUS.ConclusionsThe IDUS findings differed between PSC and IgG4‐SC. Irregular inner margin, diverticulum‐like outpouching, and disappearance of three layers are specific IDUS findings for PSC compared to IgG4‐SC. IDUS is a more useful procedure than ERC for the early detection of diverticulum‐like outpouching.
Exploring the transition experiences of students entering into preregistration nursing degree programs with previous professional nursing qualifications: an integrative review
Authors: Tower Marion , Cooke Marie , Watson Bernadette , Buys Nick , Wilson Keithia 
Published: January 2015

Aims and objectivesThis paper aims to investigate the transition experiences of students with previous nursing qualifications (nondegree qualifications) entering a nursing degree program.BackgroundNurses with professional nursing qualifications (nondegree) are an important pool of professionals to draw on to help address nursing shortages through providing alternative pathways to becoming registered nurses. However, research suggests this cohort need higher levels of support and intervention to be successful.DesignAn integrative review was conducted, guided by Whittemore and Knafl's framework. A comprehensive search was conducted and 14 studies were included in the review.MethodsA five‐stage approach was used to conduct the review: problem identification, literature search, data evaluation, data analysis and presentation of results. Constant comparative analysis was used to identify commonalities, differences and themes in the relevant literature.ResultsThemes emerged that suggested students struggled with academic and institutional challenges, becoming learners, managing conflicting demands of outside life, developing a student identity and experienced threat to their sense of professional identity. Additionally, the cohort did not view themselves as ‘the same’ as traditional students. Holland's framework provides a useful framework from which to conceptualise these students' experiences.ConclusionThere are challenges for this cohort of students as they transition into degree level study. Supportive activities that extend and further develop students' personality orientations may be a useful starting point in addressing some of the challenges and providing a more positive transition to university.Relevance to clinical practiceThe demand for health care is growing and there remains a shortfall of registered nurses. Nurses with professional qualifications (nondegree) are an important pool of professionals from which to increase registered nurse numbers and address workforce sustainability issues.
Dimensional Accuracy of Two‐Step Impressions Measured on Scanned Casts in CAD
Authors: Baráth Zoltán , Szűts Gábor , Braunitzer Gábor , Radnai Márta 
Published: January 2015

AbstractPurposeThe purpose of the study was to find out how light‐body impression material thickness influences the horizontal dimensional accuracy of casts examined on a scanned model.Materials and MethodsCustomized manikin mandibles were used for impression taking with a dual‐phase impression method. These mandibles were prepared in a way that allowed the control of light‐bodied impression material thickness. First premolars were prepared for a full crown by supragingival chamfer on both sides in all mandibles. For the measurements, buccal and lingual reference points were made at the preparation margin. Impressions were taken at nine thickness levels and a control level (TTotal = 10; 0–1.2 mm), and the reference points’ average distance was compared across thickness levels on both the left and right side. We carried out three measures for each specimen and used three specimens per thickness level. Total sample size was N = 180, creating a sizable database to understand the influence.ResultsThe results indicated that light‐body impression material thickness had no influence on the horizontal dimension of a die measured on scanned dies in these specific circumstances.ConclusionsThe thickness of light‐body impression material in the dual‐phase impression taking technique did not have a significant influence on the horizontal accuracy of scanned dies, which makes the technique safe and reliable for single crown restorations without having to take extra precautions for the thickness of light‐body impression material.
The Effect of Cyclic Loading on the Compressive Strength of Core Build‐Up Materials
Authors: Zankuli Muayed A. , Silikas Nick , Devlin Hugh 
Published: January 2015

AbstractPurposeTo evaluate the effect of cyclic loading on compressive strength of core build‐up materials.Materials and MethodsFour dual‐cured composites (Core.X Flow, Grandio Core, Bright Flow Core, Spee‐Dee) and one light‐cured reinforced resin‐modified glass ionomer (Fuji II LC) were tested. One hundred cylindrical specimens (4 mm × 6 mm) were prepared. Each material had two groups (ten specimens to be tested under static loading and ten specimens to be tested after cyclic loading). The specimens were stored wet, and after 30 days, one group of each material was cyclically loaded (for 250,000 cycles with a frequency of 1.6 Hz under stress load of 68.6 N) in a chewing simulator CS–4.2. Then specimens were subjected to static compressive loading until failure in a universal testing machine.ResultsMean compressive strength values before cycling ranged from 144 MPa (15.8) for Fuji II LC to 277 MPa (23.2) for Grandio Core. Independent t‐test showed no statistically significant difference (p > 0.05) in the compressive strength of each material before and after cycling (p = 0.7 Grandio Core, p = 0.3 Core.X Flow, p = 0.6 Bright Flow Core, p = 0.2 Spee‐Dee, p = 0.6 Fuji II LC); however, there was a statistically significant difference between the materials when comparing before and after cycling.ConclusionsAll tested materials showed no reduction in the compressive strength after cycling. Therefore, the tested materials can survive 1 year in service without a reduction in compressive strength.