bluuebunny/crossref_metadata_2025_split huggingface dataset:
Climate Change Projections in CESM1(CAM5) Compared to CCSM4
Authors: Meehl Gerald A. , Washington Warren M. , Arblaster Julie M. , Hu Aixue , Teng Haiyan , Kay Jennifer E. , Gettelman Andrew , Lawrence David M. , Sanderson Benjamin M. , Strand Warren G. 
Published: March 2013

Abstract
Future climate change projections for phase 5 of the Coupled Model Intercomparison Project (CMIP5) are presented for the Community Earth System Model version 1 that includes the Community Atmospheric Model version 5 [CESM1(CAM5)]. These results are compared to the Community Climate System Model, version 4 (CCSM4) and include simulations using the representative concentration pathway (RCP) mitigation scenarios, and extensions for those scenarios beyond 2100 to 2300. Equilibrium climate sensitivity of CESM1(CAM5) is 4.10°C, which is higher than the CCSM4 value of 3.20°C. The transient climate response is 2.33°C, compared to the CCSM4 value of 1.73°C. Thus, even though CESM1(CAM5) includes both the direct and indirect effects of aerosols (CCSM4 had only the direct effect), the overall climate system response including forcing and feedbacks is greater in CESM1(CAM5) compared to CCSM4. The Atlantic Ocean meridional overturning circulation (AMOC) in CESM1(CAM5) weakens considerably in the twenty-first century in all the RCP scenarios, and recovers more slowly in the lower forcing scenarios. The total aerosol optical depth (AOD) changes from ~0.12 in 2006 to ~0.10 in 2100, compared to a preindustrial 1850 value of 0.08, so there is less negative forcing (a net positive forcing) from that source during the twenty-first century. Consequently, the change from 2006 to 2100 in aerosol direct forcing in CESM1(CAM5) contributes to greater twenty-first century warming relative to CCSM4. There is greater Arctic warming and sea ice loss in CESM1(CAM5), with an ice-free summer Arctic occurring by about 2060 in RCP8.5 (2040s in September) as opposed to about 2100 in CCSM4 (2060s in September).
OD-characterization of the automorphism groups of simple "Equation missing" -groups
Authors: Yan Yanxiong , Xu Haijing , Chen Guiyun , He Liguan 
Published: March 2013

Abstract
The degree pattern of a finite group G associated to its prime graph has been introduced in (Moghaddamfar et al. in Algebra Colloq. 12(3):431-442, 2005) and denoted by

D
(
G
)

. The group G is called k-fold OD-characterizable if there exist exactly k non-isomorphic groups H satisfying conditions (1)

|
G
|
=
|
H
|

and (2)

D
(
G
)
=
D
(
H
)

. Moreover, a one-fold OD-characterizable group is simply called OD-characterizable group. In this problem, those groups with connected prime graphs are somewhat much difficult to be solved. In the present paper, we continue this investigation and show that the automorphism groups of simple

K
3

-groups are characterized by their orders and degree patterns. In fact, the automorphism groups of simple

K
3

-groups except

A
6

and

U
4

(
2
)

are OD-characterizable. Moreover,

Aut
(

A
6

)

is fourfold OD-characterizable and

Aut
(

U
4

(
2
)
)

is at least fourfold OD-characterizable.

AMS Subject Classification:20D05.
A heterozygous variant in the human cardiac miR-133 gene, MIR133A2, alters miRNA duplex processing and strand abundance
Authors: Ohanian Monique , Humphreys David T , Anderson Elizabeth , Preiss Thomas , Fatkin Diane 
Published: March 2013

AbstractBackgroundMicroRNAs (miRNAs) are small non-coding RNAs that post-transcriptionally regulate gene expression. Sequential cleavage of miRNA precursors results in a ~22 nucleotide duplex of which one strand, the mature miRNA, is typically loaded into the RNA-induced silencing complex (RISC) while the passenger strand is degraded. Very little is known about how genetic variation might affect miRNA biogenesis and function.ResultsWe re-sequenced theMIR1-1, MIR1-2, MIR133A1,MIR133A2, andMIR133Bgenes, that encode the cardiac-enriched miRNAs, miR-1 and miR-133, in 120 individuals with familial atrial fibrillation and identified 10 variants, including a novel 79T > CMIR133A2substitution. This variant lies within the duplex at the 3′end of the mature strand, miR-133a-3p, and is predicted to prevent base-pairing and weaken thermostability at this site, favoring incorporation of the passenger strand, miR-133a-5p, into RISC. Genomic DNA fragments containing miR-133a-2 precursor sequences with 79T and 79C alleles were transfected into HeLa cells. On Northern blotting the 79T allele showed strong expression of miR-133a-3p with weak expression of miR-133a-5p. In contrast, the 79C allele had no effect on miR-133a-3p but there was a significant increase (mean 3.6-fold) in miR-133a-5p levels. Deep sequencing of small RNA libraries prepared from normal human and murine atria confirmed that nearly all the mature miR-133a was comprised of miR-133a-3p and that levels of miR-133a-5p were very low. A number of isomiRs with variations at 5′and 3′ends were identified for both miR-133a-3p and miR-133a-5p, with 2 predominant miR-133a-3p isomiRs and one predominant miR-133a-5p isomiR. Bioinformatics analyses indicate that the major miR-133a-3p and 5p isomiRs have numerous predicted target mRNAs, only a few of which are in common.ConclusionsMultiple miR-133a isomiRs with potential different mRNA target profiles are present in the atrium in humans and mice. We identified a human 79T > CMIR133A2variant that alters miRNA processing and results in accumulation of the miR-133a-5p strand that is usually degraded.
Beverage consumption and individual-level associations in South Korea
Authors: Han Euna , Kim Tae Hyun , Powell Lisa M 
Published: March 2013

Abstract

Background
Few previous studies investigated consumption distributions of sugar-sweetened beverages (SSBs) over time and individual-level associations in Asia despite the recent interest in SSBs regarding obesity control. This study aimed to provide recent evidence on beverage consumption trends from 2001 to 2009 for overall and subtypes of SSBs and for milk as a comparable healthy beverage in South Korea, as well as associations with individual-level socioeconomic status (SES).

Methods
The Korean National Health and Nutrition Examination Surveys from 2001 to 2009 were used. Consumption prevalence and average caloric intake by SSB type were examined. Associations of SES with consumption were assessed in a multivariate logistic regression model (consumption prevalence) and in a multivariate two-part regression model (overall caloric intake adjusting for consumption probability).

Results
SSB consumption prevalence increased to 38%, 69%, 70%, and 50% by 2009 up from 31%, 66%, 63%, and 32% in 2001 among adolescents, young adults, adults, and the elderly, respectively. Miscellaneous SSBs (sports/energy drinks, coffee/tea products, flavored milk, and others) were the most prevalent and their prevalence increased among adults (from 62% to 69%) and the elderly (from 30% to 47%) between 2001 and 2009. Adolescents consumed the most calories from miscellaneous SSBs among all beverage types although its prevalence was lower than regular soda and milk in both 2001 and 2009. Women (top- income group only) and men in higher income groups showed higher odds of consuming total SSBs (OR = 1.18-1.25), soda (OR = 1.18, men only), fruit drinks (OR = 1.18, the top-income only for both genders), and miscellaneous SSBs (OR = 1.1-1.2). Men with higher-education showed higher odds of total SSB consumption (OR = 1.14-1.20), and all subtypes of SSBs (OR = 1.18, 1.29, 1.19 for soda, fruit drinks, and miscellaneous SSBs, respectively for the top-education group only). There were statistically significant but minimal differences in the overall amount of caloric intake from SSBs by individual SES for both genders.

Conclusions
South Korea is following the global nutrition transition toward greater consumption of SSBs. However, unlike other developed countries, SSB consumption prevalence was higher among high-SES individuals, particularly for fruit drinks and miscellaneous SSBs. Further research is needed to build the international evidence base.
Ethnic variation in the activity of lipid desaturases and their relationships with cardiovascular risk factors in control women and an at-risk group with previous gestational diabetes mellitus: a cross-sectional study
Authors: Gray Robert G , Kousta Eleni , McCarthy Mark I , Godsland Ian F , Venkatesan Soundarajan , Anyaoku Victor , Johnston Desmond G 
Published: March 2013

Abstract

Background
Lipid desaturase enzymes mediate the metabolism of fatty acids to long chain polyunsaturated fatty acids and their activities are related to metabolic risk factors for Type 2 diabetes (T2DM) and coronary heart disease (CHD). There are marked ethnic differences in risks of CHD and T2DM but little is known about ethnic differences in desaturase activities.

Methods
Samples from a study of CVD risk in women with previous gestational diabetes were analysed for percentage fatty acids in plasma free fatty acid, triglyceride, cholesterol ester and phospholipid pools for 89 white European, 53 African Caribbean and 56 Asian Indian women. The fatty acid desaturase activities, stearoyl-CoA desaturase (SCD, calculated separately for C16 and C18 fatty acids), delta 6 desaturase (D6D) and delta 5 desaturase (D5D) were estimated from precursor-to-product ratios and their relationships with adiposity, blood pressure, cholesterol, triglycerides, HDL cholesterol and insulin sensitivity explored. Ethnic differences in desaturase activities independent of ethnic variation in risk factor correlates of desaturase activities were then identified.

Results
There was significant ethnic variation in age, BMI, waist circumference, blood pressure, serum triglycerides and HDL cholesterol concentrations and insulin resistance. Desaturase activities showed significant correlations, independent of ethnicity, with BMI, waist circumference, triglycerides and HDL cholesterol. Independent of ethnic variation in BMI, waist circumference, triglycerides and HDL cholesterol, SCD-16 activity, calculated from each of the four lipid pools measured, was 18–35 percent higher in white Europeans than in African Caribbeans or Asian Indians (all p < 0.001). Similar, though less consistent differences were apparent for SCD-18 activity. Also independently of risk factor variation, but specifically when calculated from the cholesterol ester and phospholipid, pools, D6D activity was significantly lower in Asian Indians, and D5D activity higher in African Caribbeans.

Conclusions
Significant ethnic differences exist in desaturase activities, independently of ethnic variation in other risk factors. These characteristics did not accord with higher risk of T2DM among African Caribbeans and Asian Indians nor with lower risk of CHD among African Caribbeans but did accord with the higher risk of CHD in Asian Indians.
Micronutrients-fortified rapeseed oil improves hepatic lipid accumulation and oxidative stress in rats fed a high-fat diet
Authors: Xu Jiqu , Zhou Xiaoqi , Gao Hui , Chen Chang , Deng Qianchun , Huang Qingde , Ma Jing , Wan Zhengyang , Yang Jin’e , Huang Fenghong 
Published: March 2013

Abstract
Intake of high-fat diet is associated with increased fatty livers. Hepatic lipid accumulation and oxidative stress are key pathophysiological mechanisms in this disease. Micronutrients polyphenols, tocopherols and phytosterols in rapeseed exert potential benefit to hepatoprotection, but most of these micronutrients are removed by the traditional refining process. The purpose of the present study was to determine whether rapeseed oil fortified with these micronutrients can decrease hepatic lipid accumulation and oxidative stress induced by high-fat diet. Sprague–Dawley rats received rodent diet contained 20% fat whose source was refined rapeseed oil (RRO) or fortified RRO with low, middle and high quantities of these micronutrients for 10 weeks. Intake of RRO caused a remarkable hepatic steatosis. Micronutrients supplementation was effective in reducing steatosis as well as total triglyceride and total cholesterol contents in liver. These micronutrients also significantly increased hepatic antioxidant defense capacities, as evaluated by the significant elevation in the activities of SOD and GPx as well as the level of GSH, and the significant decline in lipid peroxidation. These findings suggest that rapeseed oil fortified with micronutrients polyphenols, tocopherols and phytosterols may contribute to prevent fatty livers such as nonalcoholic fatty liver disease by ameliorating hepatic lipid accumulation and oxidative stress.
Variability between laboratories performing coagulation tests with identical platforms: a nationwide evaluation study
Authors: Nagler Michael , Bachmann Lucas M , Alberio Lorenzo , Angelillo-Scherrer Anne , Asmis Lars M , Korte Wolfgang , Mendez Adriana , Reber Guido , Stricker Hans , Tsakiris Dimitrios A , Wuillemin Walter A 
Published: March 2013

AbstractBackgroundWhile the assessment of analytical precision within medical laboratories has received much attention in scientific enquiry, the degree of as well as the sources causing variation between them remains incompletely understood. In this study, we quantified the variance components when performing coagulation tests with identical analytical platforms in different laboratories and computed intraclass correlations coefficients (ICC) for each coagulation test.MethodsData from eight laboratories measuring fibrinogen twice in twenty healthy subjects with one out of 3 different platforms and single measurements of prothrombin time (PT), and coagulation factors II, V, VII, VIII, IX, X, XI and XIII were analysed. By platform, the variance components of (i) the subjects, (ii) the laboratory and the technician and (iii) the total variance were obtained for fibrinogen as well as (i) and (iii) for the remaining factors using ANOVA.ResultsThe variability for fibrinogen measurements within a laboratory ranged from 0.02 to 0.04, the variability between laboratories ranged from 0.006 to 0.097. The ICC for fibrinogen ranged from 0.37 to 0.66 and from 0.19 to 0.80 for PT between the platforms. For the remaining factors the ICC’s ranged from 0.04 (FII) to 0.93 (FVIII).ConclusionsVariance components that could be attributed to technicians or laboratory procedures were substantial, led to disappointingly low intraclass correlation coefficients for several factors and were pronounced for some of the platforms. Our findings call for sustained efforts to raise the level of standardization of structures and procedures involved in the quantification of coagulation factors.
No Evidence for the Development of Acute Analgesic Tolerance during and Hyperalgesia after Prolonged Remifentanil Administration in Mice
Authors: Ishii Hideaki , Petrenko Andrey B , Kohno Tatsuro , Baba Hiroshi 
Published: March 2013

Background Acute opioid tolerance (AOT) and opioid-induced hyperalgesia (OIH) are undesirable effects of opioids that have been reported in both animals and humans. However, the development of AOT and OIH in cases of potent, short-acting μ-opioid receptor agonist remifentanil administration remains controversial. It has been suggested that the emergence of AOT and OIH by remifentanil could be dose and infusion duration dependent, i.e., low dose and short infusions may lead to negative results. In this study, we determined whether AOT and OIH could be elicited by prolonged, continuous administration of remifentanil at maximally tolerable doses in C57BL/6 mice. Results The analgesic effects of continuously administered remifentanil [by short (1 h) and prolonged (4 h) intraperitoneal infusions] were studied. These experiments involved repeated measurements of thermal thresholds during remifentanil administration. Therefore, particular attention was paid to prevent cumulative tissue injury, which could mimic pronociceptive effects of remifentanil. To exclude the possibility of pseudoAOT during infusion, we used brief cooling of all ipsilateral hindpaws that exhibited analgesic response. Thermal thresholds remained steadily elevated over a 1-h period during continuous administration at infusion rates of 120, 180, and 240 mg/kg/h, which indicated no AOT development. To exclude the possibility of pseudoOIH after infusion, intact contralateral hindpaws were used for all postinfusion threshold measurements. Thermal thresholds at each infusion rate returned to the baseline values within 15 min after the termination of the administration. They did not decrease below the baseline values during 1 h following infusion, which indicated no OIH development. Similar threshold dynamics were also observed for thermal and mechanical testing modalities in animals infused at 120 mg/kg/h for 4 h as well as in animals with rapidly attained and maintained maximum analgesia for 3 h. Conclusions These results suggest that neither intra-infusion AOT nor postinfusion OIH develops in mice receiving continuous remifentanil when the possibility of cumulative tissue injury mimicking AOT or OIH is carefully avoided.
Renaissance of base deficit for the initial assessment of trauma patients: a base deficit-based classification for hypovolemic shock developed on data from 16,305 patients derived from the TraumaRegister DGU®
Authors: Mutschler Manuel , Nienaber Ulrike , Brockamp Thomas , Wafaisade Arasch , Fabian Tobias , Paffrath Thomas , Bouillon Bertil , Maegele Marc ,   the TraumaRegister DGU
Published: March 2013

Abstract
Introduction
The recognition and management of hypovolemic shock still remain an important task during initial trauma assessment. Recently, we have questioned the validity of the Advanced Trauma Life Support (ATLS) classification of hypovolemic shock by demonstrating that the suggested combination of heart rate, systolic blood pressure and Glasgow Coma Scale displays substantial deficits in reflecting clinical reality. The aim of this study was to introduce and validate a new classification of hypovolemic shock based upon base deficit (BD) at emergency department (ED) arrival.

Methods
Between 2002 and 2010, 16,305 patients were retrieved from the TraumaRegister DGU® database, classified into four strata of worsening BD [class I (BD ≤ 2 mmol/l), class II (BD > 2.0 to 6.0 mmol/l), class III (BD > 6.0 to 10 mmol/l) and class IV (BD > 10 mmol/l)] and assessed for demographics, injury characteristics, transfusion requirements and fluid resuscitation. This new BD-based classification was validated to the current ATLS classification of hypovolemic shock.

Results
With worsening of BD, injury severity score (ISS) increased in a step-wise pattern from 19.1 (± 11.9) in class I to 36.7 (± 17.6) in class IV, while mortality increased in parallel from 7.4% to 51.5%. Decreasing hemoglobin and prothrombin ratios as well as the amount of transfusions and fluid resuscitation paralleled the increasing frequency of hypovolemic shock within the four classes. The number of blood units transfused increased from 1.5 (± 5.9) in class I patients to 20.3 (± 27.3) in class IV patients. Massive transfusion rates increased from 5% in class I to 52% in class IV. The new introduced BD-based classification of hypovolemic shock discriminated transfusion requirements, massive transfusion and mortality rates significantly better compared to the conventional ATLS classification of hypovolemic shock (p < 0.001).

Conclusions
BD may be superior to the current ATLS classification of hypovolemic shock in identifying the presence of hypovolemic shock and in risk stratifying patients in need of early blood product transfusion.
Development of fully defined xeno-free culture system for the preparation and propagation of cell therapy-compliant human adipose stem cells
Authors: Patrikoski Mimmi , Juntunen Miia , Boucher Shayne , Campbell Andrew , Vemuri Mohan C , Mannerström Bettina , Miettinen Susanna 
Published: March 2013

Abstract
Introduction
Adipose tissue is an attractive and abundant source of multipotent stem cells. Human adipose stem cells (ASCs) have shown to have therapeutic relevancy in diverse clinical applications. Nevertheless, expansion of ASCs is often necessary before performing clinical studies. Standard in vitro cell-culture techniques use animal-derived reagents that should be avoided in clinical use because of safety issues. Therefore, xeno- and serum-free (XF/SF) reagents are highly desirable for enhancing the safety and quality of the transplanted ASCs.

Methods
In the current study, animal component-free isolation and cell-expansion protocols were developed for ASCs. StemPro MSC SFM XF medium with either CELLstart™ CTS™ coating or Coating Matrix Kit were tested for their ability to support XF/SF growth. Basic stem-cell characteristics such as immunophenotype (CD3, CD11a, CD14, CD19, CD34, CD45RO, CD54, CD73, CD80, CD86, CD90, CD105, HLA-DR), proliferation, and differentiation potential were assessed in XF/SF conditions and compared with human serum (HS) or traditionally used fetal bovine serum (FBS) cultures.

Results
ASCs cultured in XF/SF conditions had significantly higher proliferation rates compared with HS/FBS cultures. Characteristic immunophenotypes of ASCs were maintained in every condition; however, cells expanded in XF/SF conditions showed significantly lower expression of CD54 (intercellular adhesion molecule 1, ICAM-1) at low passage number. Further, multilineage differentiation potential of ASCs was maintained in every culture condition.

Conclusions
Our findings demonstrated that the novel XF/SF conditions maintained the basic stem cell features of ASCs and the animal-free workflow followed in this study has great potential in clinical cell therapies.
Radioactive iodine in the treatment of medullary thyroid carcinoma: a controlled multicenter study
Authors: Meijer J A A , Bakker L E H , Valk G D , de Herder W W , de Wilt J H W , Netea-Maier R T , Schaper N , Fliers E , Lips P , Plukker J T , Links T P , Smit J A 
Published: March 2013

ObjectiveRadioactive iodine (RAI) therapy in medullary thyroid carcinoma (MTC) is applied in some centers, based on the assumption that cross-irradiation from thyroid follicular cells may be beneficial. However, no systematic studies on the effect of RAI treatment in MTC have been performed. The aim of this study was to analyze the effect of RAI treatment on survival in MTC patients.DesignRetrospective multicenter study in eight University Medical Centers in The Netherlands.MethodsTwo hundred and ninety three MTC patients without distant metastases who had undergone a total thyroidectomy were included between 1980 and 2007. Patients were stratified by clinical appearance, hereditary stage, screening status, and localization. All patients underwent regular surgical treatment with additional RAI treatment in 61 patients. Main outcome measures were disease-free survival (DFS) and disease-specific survival (DSS). Cure was defined as biochemical and radiological absence of disease.ResultsIn multivariate analysis, stratification according to clinical appearance (P=0.72), hereditary stage (P=0.96), localization (P=0.69), and screening status (P=0.31) revealed no significant effects of RAI treatment on DFS. Multivariate analysis showed no significant difference in DSS for the two groups stratified according to clinical appearance (P=0.14). Owing to limited number of events, multivariate analysis was not possible for DSS in the other groups of stratification.ConclusionsBased on the results of the present analysis, we conclude that RAI has no place in the treatment of MTC.