bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic association studies of ACE and PAI-1 genes in women with recurrent pregnancy loss
Authors: Su Mei-Tsz , Lin Sheng-Hsiang , Chen Yi-Chi , Kuo Pao-Lin 
Published: November 2012

SummaryA fine balance between coagulation and fibrinolysis is critical in early pregnancy. Plasminogen activator inhibitor-1 (PAI-1) and angiotensin converting enzyme (ACE) are involved in the fibrinolytic process, and several studies have reported the association between their gene polymorphisms and recurrent pregnancy loss (RPL). This study was conducted to investigate the association between PAI-1 and ACE polymorphisms and idiopathic RPL, using meta-analyses. A systematic review of the published literature from the MEDLINE and EMBASE databases before April 2012 was conducted. Of 209 potentially relevant studies, 22 case-control studies comprising a total of 2,820 RPL patients and 3,009 controls were included. Among these studies were 11 reports of PAI-1 4G/5G and 11 of ACE I/D polymorphisms in patients with RPL. A significant association was found withthe ACE I/D polymorphism [summary odds ratio 1.29 (95% confidence interval 1.02–1.62)] in studies including more than two recurrent abortions. Subgroup analysis did not show significant associations with RPL in Caucasian and non-Caucasian patients. Meta-analyses of PAI-1 4G/5G polymorphism were not found associations with RPL in studies including more than two or three recurrent abortions, and in studies of Caucasian and non-Caucasian patients. In conclusion, meta-analyses showed a significant association between the ACE I/D polymorphism and idiopathic RPL. High clinical heterogeneity existed among studies of PAI-1 4G/5G, and the aggregated data failed to confer higher susceptibility to idiopathic RPL. More well-designed studies with different ethnic populations are required for future integration.
Extensive White Matter Hyperintensities May Increase Brain Volume in Cerebral Autosomal-Dominant Arteriopathy With Subcortical Infarcts and Leukoencephalopathy
Authors: Yao Ming , Jouvent Eric , During Marco , Godin Ophélia , Hervé Dominique , Guichard Jean Pierre , Zhu Yi-Cheng , Gschwendtner Andreas , Opherk Christian , Dichgans Martin , Chabriat Hugues 
Published: November 2012

Background and Purpose—
The extent of white matter hyperintensities (WMH) is associated with cerebral atrophy in elderly people. WMH is a radiological hallmark of cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL), but their relationship with brain volume remains poorly understood. The association between WMH and brain volume was analyzed in a large population of patients with CADASIL.

Methods—
Demographic and MRI data of 278 patients recruited from a prospective cohort study were analyzed. Volumes of WMH and lacunar infarcts, number of cerebral microbleeds, and brain parenchymal fraction were measured. Multivariate analysis was used to study the impact of WMH on brain volume at baseline.

Results—

In univariate analyses, brain parenchymal fraction was negatively associated with age, male sex, and all MRI markers. Multiple regression modeling showed that brain parenchymal fraction was inversely related to age, number of cerebral microbleeds, and normalized volume of lacunar infarcts but positively related to normalized volume of WMH (
P
<0.001). This positive relationship was independent of the presence/absence of lacunar infarcts or of cerebral microbleeds. Subgroup analysis showed that this association was significant in subjects having normalized volume of WMH ≥6.13 or brain parenchymal fraction ≥86.37% (median values, both
P
≤0.001).

Conclusion—
The results of the present study suggest that extensive WMH may be associated with increase of brain volume in CADASIL. In this disorder, WMH may be related not only to loss of white matter components, but also to a global increase of water content in the cerebral tissue.
Spatial Decomposition of Climate Feedbacks in the Community Earth System Model
Authors: Gettelman A. , Kay J. E. , Fasullo J. T. 
Published: November 2012

Abstract
An ensemble of simulations from different versions of the Community Atmosphere Model in the Community Earth System Model (CESM) is used to investigate the processes responsible for the intermodel spread in climate sensitivity. In the CESM simulations, the climate sensitivity spread is primarily explained by shortwave cloud feedbacks on the equatorward flank of the midlatitude storm tracks. Shortwave cloud feedbacks have been found to explain climate sensitivity spread in previous studies, but the location of feedback differences was in the subtropics rather than in the storm tracks as identified in CESM. The cloud-feedback relationships are slightly stronger in the winter hemisphere. The spread in climate sensitivity in this study is related both to the cloud-base state and to the cloud feedbacks. Simulated climate sensitivity is correlated with cloud-fraction changes on the equatorward side of the storm tracks, cloud condensate in the storm tracks, and cloud microphysical state on the poleward side of the storm tracks. Changes in the extent and water content of stratiform clouds (that make up cloud feedback) are regulated by the base-state vertical velocity, humidity, and deep convective mass fluxes. Within the storm tracks, the cloud-base state affects the cloud response to CO2-induced temperature changes and alters the cloud feedbacks, contributing to climate sensitivity spread within the CESM ensemble.
Coupled Sea Ice–Ocean-State Estimation in the Labrador Sea and Baffin Bay
Authors: Fenty Ian , Heimbach Patrick 
Published: November 2012

Abstract
Sea ice variability in the Labrador Sea is of climatic interest because of its relationship to deep convection, mode-water formation, and the North Atlantic atmospheric circulation. Historically, quantifying the relationship between sea ice and ocean variability has been difficult because of in situ observation paucity and technical challenges associated with synthesizing observations with numerical models. Here the relationship between ice and ocean variability is explored by analyzing new estimates of the ocean–ice state in the northwest North Atlantic. The estimates are syntheses of in situ and satellite hydrographic and ice data with a regional ⅓° coupled ocean–sea ice model. The synthesis of sea ice data is achieved with an improved adjoint of a thermodynamic ice model. Model and data are made consistent, in a least squares sense, by iteratively adjusting control variables, including ocean initial and lateral boundary conditions and the atmospheric state, to minimize an uncertainty-weighted model–data misfit cost function. The utility of the state estimate is demonstrated in an analysis of energy and buoyancy budgets in the marginal ice zone (MIZ). In mid-March the system achieves a state of quasi-equilibrium during which net ice growth and melt approaches zero; newly formed ice diverges from coastal areas and converges via wind and ocean forcing in the MIZ. The convergence of ice mass in the MIZ is ablated primarily by turbulent ocean–ice enthalpy fluxes. The primary source of the enthalpy required for sustained MIZ ice ablation is the sensible heat reservoir of the subtropical-origin subsurface waters.
Efficacy of High-Frequency Repetitive Transcranial Magnetic Stimulation in Treatment-Resistant Depression
Authors: Tarhan Nevzat , Sayar F. Gökben Hızlı , Tan Oğuz , Kağan Gaye 
Published: November 2012

We examined the efficacy of high-frequency repetitive transcranial magnetic stimulation (rTMS) in 419 patients with treatment-resistant depression. The patients received daily sessions of rTMS over the left prefrontal cortex as an adjuvant to pharmacotherapy. The rTMS intensity was set at 100% of the motor threshold and 25 Hz stimulation, with train duration of 2 seconds delivered at 30-second intervals. A full course comprised 1000 magnetic pulses. Depression was rated using the Hamilton Depression Rating Scale (HAMD) before and after treatment. Response was defined as a 50% reduction in the HAMD score. Patients with HAMD scores of less than 8 were considered to be in remission. The mean HAMD score for the study group decreased from 22.59 ± 5.92 to 10.50 ± 5.83 ( P < .001). After the treatment period, 268 (64%) out of 419 patients demonstrated significant mood improvements, as indexed by a reduction of more than 50% on the HAMD score. In addition, 140 patients (33.4%) attained remission (HAMD score <8); and 11 patients achieved a partial response. Treatment was generally well tolerated, and no serious adverse effects were reported. In conclusion, high-frequency (25 Hz) rTMS was well tolerated and found to be statistically and clinically effective in patients with treatment-resistant depression. This study contributed to the existing evidence of the antidepressant effect of rTMS in the treatment of depression.
Modelling and estimation of genotype by environment interactions for production traits in French dairy cattle
Authors: Huquet Bérénice , Leclerc Hélène , Ducrocq Vincent 
Published: November 2012

Abstract

Background
Genotype by environment interactions are currently ignored in national genetic evaluations of dairy cattle. However, this is often questioned, especially when environment or herd management is wide-ranging. The aim of this study was to assess genotype by environment interactions for production traits (milk, protein, fat yields and fat and protein contents) in French dairy cattle using an original approach to characterize the environments.

Methods
Genetic parameters of production traits were estimated for three breeds (Holstein, Normande and Montbéliarde) using multiple-trait and reaction norm models. Variables derived from Herd Test Day profiles obtained after a test day model evaluation were used to define herd environment.

Results
Multiple-trait and reaction norm models gave similar results. Genetic correlations were very close to unity for all traits, except between some extreme environments. However, a relatively wide range of heritabilities by trait and breed was found across environments. This was more the case for milk, protein and fat yields than for protein and fat contents.

Conclusions
No real reranking of animals was observed across environments. However, a significant scale effect exists: the more intensive the herd management for milk yield, the larger the heritability.
Mode of primary cancer detection as an indicator of screening practice for second primary cancer in cancer survivors: a nationwide survey in Korea
Authors: Suh Beomseok , Shin Dong Wook , Kim So Young , Park Jae-Hyun , Chang Weon Young , Lim Seung Pyung , Yim Chang-Yeol , Cho Be-Long , Park Eun-Cheol , Park Jong-Hyock 
Published: November 2012

Abstract

Background
While knowledge and risk perception have been associated with screening for second primary cancer (SPC), there are no clinically useful indicators to identify who is at risk of not being properly screened for SPC. We investigated whether the mode of primary cancer detection (i.e. screen-detected vs. non-screen-detected) is associated with subsequent completion of all appropriate SPC screening in cancer survivors.

Methods
Data were collected from cancer patients treated at the National Cancer Center and nine regional cancer centers across Korea. A total of 512 cancer survivors older than 40, time since diagnosis more than 2 years, and whose first primary cancer was not advanced or metastasized were selected. Multivariate logistic regression was used to examine factors, including mode of primary cancer detection, associated with completion of all appropriate SPC screening according to national cancer screening guidelines.

Results
Being screen-detected for their first primary cancer was found to be significantly associated with completion of all appropriate SPC screening (adjusted odds ratio, 2.13; 95% confidence interval, 1.36–3.33), after controlling for demographic and clinical variables. Screen-detected cancer survivors were significantly more likely to have higher household income, have other comorbidities, and be within 5 years since diagnosis.

Conclusions
The mode of primary cancer detection, a readily available clinical information, can be used as an indicator for screening practice for SPC in cancer survivors. Education about the importance of SPC screening will be helpful particularly for cancer survivors whose primary cancer was not screen-detected.
Chemo-enzymatic synthesis and in vitro cytokine profiling of tailor-made oligofructosides
Authors: Homann Arne , Timm Malte , Seibel Jürgen 
Published: November 2012

Abstract

Background
It is well known that carbohydrates play fundamental roles in cell signaling and infection processes as well as tumor formation and progression. However, the interaction pathways and cellular receptors targeted by carbohydrates and glycoconjugates remain poorly examined and understood. This lack of research stems, at least to a major part, from accessibility problems of large, branched oligosaccharides.

Results
To test glycan - cell interactions in vitro, a variety of tailored oligosaccharides was synthesized chemo-enzymatically. Glycosyltransferases from the GRAS organisms Bacillus megaterium (SacB) and Aspergillus niger (Suc1) were used in this study. Substrate engineering of these glycosyltransferases generally acting on sucrose leads to the controlled formation of novel tailored di-, tri- and tetrasaccharides. Already industrially used as prebiotics in functional food, the immunogenic potential of novel oligosaccharides was characterized in this study. A differential secretion of CXCL8 and CCL2 was observed upon oligosaccharide co-cultivation with colorectal epithelial Caco-2 cells.

Conclusion
Pure carbohydrates are able to stimulate a cytokine response in human endothelial cells in vitro. The type and amount of cytokine secretion depends on the type of co-cultivated oligosaccharide.
Genotype comparison of Plasmodium vivax and Plasmodium falciparum clones from pregnant and non-pregnant populations in North-west Colombia
Authors: Arango Eliana M , Samuel Roshini , Agudelo Olga M , Carmona-Fonseca Jaime , Maestre Amanda , Yanow Stephanie K 
Published: November 2012

AbstractBackgroundPlacental malaria is the predominant pathology secondary to malaria in pregnancy, causing substantial maternal and infant morbidity and mortality in tropical areas. While it is clear that placental parasites are phenotypically different from those in the peripheral circulation, it is not known whether unique genotypes are associated specifically with placental infection or perhaps more generally with pregnancy. In this study, genetic analysis was performed onPlasmodium vivaxandPlasmodium falciparumparasites isolated from peripheral and placental blood in pregnant women living in North-west Colombia, and compared with parasites causing acute malaria in non-pregnant populations.MethodsA total of 57 pregnant women at delivery with malaria infection confirmed by real-time PCR in peripheral or placental blood were included, as well as 50 pregnant women in antenatal care and 80 men or non-pregnant women with acute malaria confirmed by a positive thick smear forP. vivaxorP. falciparum. Five molecular markers per species were genotyped by nested PCR and capillary electrophoresis. Genetic diversity and the fixation index FSTper species and study group were calculated and compared.ResultsAlmost all infections at delivery were asymptomatic with significantly lower levels of infection compared with the groups with acute malaria. Expected heterozygosity forP. vivaxmolecular markers ranged from 0.765 to 0.928 and forP. falciparummarkers ranged from 0.331 to 0.604. ForP. vivaxinfections, the genetic diversity was similar amongst the four study groups and the fixation index from each pairwise comparison failed to show significant genetic differentiation. ForP. falciparum, no genetic differentiation was observed between placental and peripheral parasites from the same woman at delivery, but the parasites isolated at delivery showed significant genetic differentiation compared with parasites isolated from subjects with acute malaria.ConclusionsIn North-west Colombia,P. vivaxparasites have high genetic diversity that is equivalent in pregnant and non-pregnant populations as well as in symptomatic and asymptomatic infections. ForP. falciparum, the overall genetic diversity is lower, with specific genotypes associated with asymptomatic infections at delivery.
Formation of Ga droplets on patterned GaAs (100) by molecular beam epitaxy
Authors: Li Ming-Yu , Hirono Yusuke , Koukourinkova Sabina D , Sui Mao , Song Sangmin , Kim Eun-Soo , Lee Jihoon , Salamo Gregory J 
Published: November 2012

Abstract
In this paper, the formation of Ga droplets on photo-lithographically patterned GaAs (100) and the control of the size and density of Ga droplets by droplet epitaxy using molecular beam epitaxy are demonstrated. In extension of our previous result from the journal Physical Status Solidi A, volume 209 in 2012, the sharp contrast of the size and density of Ga droplets is clearly observed by high-resolution scanning electron microscope, atomic force microscope, and energy dispersive X-ray spectrometry. Also, additional monolayer (ML) coverage is added to strength the result. The density of droplets is an order of magnitude higher on the trench area (etched area), while the size of droplets is much larger on the strip top area (un-etched area). A systematic variation of ML coverage results in an establishment of the control of size and density of Ga droplets. The cross-sectional line profile analysis and root mean square roughness analysis show that the trench area (etched area) is approximately six times rougher. The atomic surface roughness is suggested to be the main cause of the sharp contrast of the size and density of Ga droplets and is discussed in terms of surface diffusion.
Growth and characterization of thin Cu-phthalocyanine films on MgO(001) layer for organic light-emitting diodes
Authors: Bae Yu Jeong , Lee Nyun Jong , Kim Tae Hee , Cho Hyunduck , Lee Changhee , Fleet Luke , Hirohata Atsufumi 
Published: November 2012

Abstract
Surface morphology and thermal stability of Cu-phthalocyanine (CuPc) films grown on an epitaxially grown MgO(001) layer were investigated by using atomic force microscope and X-ray diffractometer. The (002) textured β phase of CuPc films were prepared at room temperature beyond the epitaxial MgO/Fe/MgO(001) buffer layer by the vacuum deposition technique. The CuPc structure remained stable even after post-annealing at 350°C for 1 h under vacuum, which is an important advantage of device fabrication. In order to improve the device performance, we investigated also current-voltage-luminescence characteristics for the new top-emitting organic light-emitting diodes with different thicknesses of CuPc layer.
On fixed points of α-ψ-contractive multifunctions
Authors: Asl J Hasanzade , Rezapour S , Shahzad N 
Published: November 2012

Abstract
Recently Samet, Vetro and Vetro introduced the notion of α-ψ-contractive type mappings and established some fixed point theorems in complete metric spaces. In this paper, we introduce the notion of

α
∗

-
ψ

-contractive multifunctions and give a fixed point result for these multifunctions. We also obtain a fixed point result for self-maps in complete metric spaces satisfying a contractive condition.
Phylogenetic diversity and genotypic complexity of H1N1 subtype swine influenza viruses isolated in Mainland China
Authors: Liu Yizhi , Wang Jing , Ji Jun , Chang Shuang , Xue Chunyi , Ma Jingyun , Bi Yingzuo , Xie Qingmei 
Published: November 2012

Abstract

Background
After the occurrence of 2009 pandemic H1N1, close attention has been paid to the H1N1 subtype swine influenza viruses (H1N1 SIV) by scientific communities in many countries. A large-scale sequence analysis of the NCBI Influenza Virus Resource Database on H1N1 SIVs submitted primarily by scientists in China during 1992 to 2011 was performed. The aims of this study were to elucidate the genetic and evolutionary characteristics of H1N1 SIVs, to identify and unify the lineages and genetic characteristics of the H1N1 SIVs isolated in mainland China.

Results
Most of the strains were isolated during the period of 2008 to 2010 from Guangdong and Shandong provinces, China. Based on the phylogenetic and genotypic analyses, all of the H1N1 SIV strains can be classified into 8 lineages and 10 genotypes. All strains were of the characteristics of low pathogenic influenza viruses. The viruses of different lineage are characterized with different amino acid residues at the receptor-binding sites. Viruses containing PB2 genes of the classical swine, early seasonal human and recent seasonal human lineage might be more infectious to human. Some genotypes were directly related with human influenza viruses, which include strains that harbored genes derived from human influenza viruses.

Conclusions
Phylogenetic diversity and complexity existed in H1N1 SIVs isolated in mainland China. These H1N1 SIV strains were closely related to other subtype influenza viruses, especially to human influenza viruses. Moreover, it was shown that, novel lineages and genotypes of H1N1 SIVs emerged recently in mainland China. These findings provided new and essential information for further understanding of the genetic and evolutionary characteristics and monitoring the H1N1 SIVs in mainland China.
Performance and diagnostic usefulness of commercially available enzyme linked immunosorbent assay and rapid kits for detection of HIV, HBV and HCV in India
Authors: Maity Susmita , Nandi Srijita , Biswas Subrata , Sadhukhan Salil Kumar , Saha Malay Kumar 
Published: November 2012

AbstractBackgroundHIV, HBV and HCV pose a major public health problem throughout the world. Detection of infection markers for these agents is a major challenge for testing laboratories in a resource poor setting. As blood transfusion is an important activity saving millions of live every year, it also carries a risk of transfusion transmissible infections caused by these fatal blood borne pathogens if the quality of testing is compromised. Conventional ELISA is regarded as the mostly used screening technique but due to limitations like high cost, unavailability in many blood banks and testing sites, involvement of costly instruments, time taking nature and requirement of highly skilled personnel for interpretation, rapid tests are gaining more importance and warrants comparison of performance.ResultsA comparative study between these two techniques has been performed using commercially available diagnostic kits to assess their efficacy for detection of HIV, HBV and HCV infections. Rapid kits were more efficient in specificity with synthetic antigens along with high PPV than ELISA in most cases. Comparison between different ELISA kits revealed that Microlisa HIV and Hepalisa (J. Mitra & Co. Pvt. Ltd.); ERBA LISA HIV1 + 2, ERBA LISA Hepatitis B and ERBA LISA HCV (Transasia Bio-medicals Ltd.) gives uniform result with good performance in terms of sensitivity, specificity, PPV, NPV and efficiency, whereas, Microlisa HCV (J. Mitra & Co. Pvt. Ltd.), Microscreen HBsAg ELISA and INNOVA HCV (Span Diagnostics Ltd.) did not perform well. Rapid kits were also having high degree of sensitivity and specificity (100%) except in HIV Comb and HCV Comb (J. Mitra & Co. Pvt. Ltd.). The kit efficiency didn’t vary significantly among different companies and lots in all the cases except for HCV ELISA showing statistically significant variation (p < 0.01) among three kit types.ConclusionsELISA is a good screening assay for markers of HIV, HBV and HCV infections. Rapid tests are useful for further detection of false positive samples. ELISA seems the appropriate assay in blood bank. For availability of quality commercial diagnostic assays, evaluation of kit may be helpful.