bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic induction of apoptosis in human leukemia cells (U937) exposed to bryostatin 1 and the proteasome inhibitor lactacystin involves dysregulation of the PKC/MAPK cascade
Authors: Vrana Julie A. , Grant Steven 
Published: July 2002

AbstractCotreatment with a minimally toxic concentration of the protein kinase C (PKC) activator (and down-regulator) bryostatin 1 (BRY) induced a marked increase in mitochondrial dysfunction and apoptosis in U937 monocytic leukemia cells exposed to the proteasome inhibitor lactacystin (LC). This effect was blocked by cycloheximide, but not by α-amanitin or actinomycin D. Qualitatively similar interactions were observed with other PKC activators (eg, phorbol 12-myristate 13-acetate and mezerein), but not phospholipase C, which does not down-regulate the enzyme. These events were examined in relationship to functional alterations in stress (eg, SAPK, JNK) and survival (eg, MAPK, ERK) signaling pathways. The observations that LC/BRY treatment failed to trigger JNK activation and that cell death was unaffected by a dominant-interfering form of c-JUN (TAM67) or by pretreatment with either curcumin or the p38/RK inhibitor, SB203580, suggested that the SAPK pathway was not involved in potentiation of apoptosis. In marked contrast, perturbations in the PKC/Raf/MAPK pathway played an integral role in LC/BRY-mediated cell death based on evidence that pretreatment of cells with bisindolylmaleimide I, a selective PKC inhibitor, or geldanamycin, a benzoquinone ansamycin, which destabilizes and depletes Raf-1, markedly suppressed apoptosis. Furthermore, ERK phosphorylation was substantially prolonged in LC/BRY-treated cells compared to those exposed to BRY alone, and pretreatment with the highly specific MEK inhibitors, PD98059, U0126, and SL327, opposed ERK activation while protecting cells from LC/BRY-induced lethality. Together, these findings suggest a role for activation and/or dysregulation of the PKC/MAPK cascade in modulation of leukemic cell apoptosis following exposure to the proteasome inhibitor LC.
Analysis of BCL-6 mutations in classic Hodgkin disease of the B- and T-cell type
Authors: Seitz Volkhard , Hummel Michael , Anagnostopoulos Ioannis , Stein Harald 
Published: July 2002

Abstract
BCL-6 is essential for germinal center formation and thus for affinity maturation of immunoglobulin (Ig) genes by somatic mutations. The 5′-noncoding region of the BCL-6gene is even a target for the mutation machinery. Translocations of theBCL-6 gene to heterologous promoters and mutations of its 5′-noncoding regulatory region were reported to be potential mechanisms for deregulating BCL-6 expression and for playing a role in the genesis of non-Hodgkin lymphoma. In line with this hypothesis is the observation that B-cell lymphoma with somatic mutations, such as diffuse large B-cell lymphoma and follicular lymphoma, also carryBCL-6 mutations, some of which are recurrently detectable. Classic Hodgkin disease (cHD) is also derived from B cells with high loads of somatic mutations and thus a further candidate forBCL-6 mutations. To determine the presence and potential role of BCL-6 mutations in cHD, the 5′-noncodingBCL-6 proportion of single Hodgkin and Reed-Sternberg (HRS) cells from 6 cases of cHD and 6 cases of HD-derived cell lines was analyzed. All B-cell–derived HD cases and cell lines harboredBCL-6 mutations. In contrast, both T-cell–derived HD cases and cell lines were devoid of BCL-6 mutations. With only one exception, there were no lymphoma-specific recurrentBCL-6 mutations detected, and BCL-6 protein was absent from the HRS cells of most cases. In conclusion, (1) somaticBCL-6 mutations are restricted to cHD cases of B-cell origin, and (2) the BCL-6 mutations represent mostly irrelevant somatic base substitutions without consequences for BCL-6 protein expression and the pathogenesis of cHD.
Temporal differences in membrane loss lead to distinct reticulocyte features in hereditary spherocytosis and in immune hemolytic anemia
Authors: Da Costa Lydie , Mohandas Narla , Sorette Martin , Grange Marie-José , Tchernia Gil , Cynober Thérèse 
Published: July 2002

AbstractSpherocytic red cells with reduced membrane surface area are a feature of hereditary spherocytosis (HS) and some forms of autoimmune hemolytic anemia (AIHA). It is generally assumed that membrane loss in spherocytic red cells occurs during their sojourn in circulation. The structural basis for membrane loss in HS is improper assembly of membrane proteins, whereas in AIHA it is due to partial phagocytosis of circulating red cells by macrophages. A hypothesis was formed that these different mechanisms should lead to temporal differences in surface area loss during red cell genesis and during sojourn in circulation in these 2 spherocytic syndromes. It was proposed that cell surface loss could begin at the reticulocyte stage in HS, whereas surface area loss in AIHA involves only circulating mature red cells. The validity of this hypothesis was established by documenting differences in cellular features of reticulocytes in HS and AIHA. Using a novel technique to quantitate cell surface area, the decreased membrane surface area of both reticulocytes and mature red cells in HS compared with normal cells was documented. In contrast, in AIHA only mature red cells but not reticulocytes exhibited decreased membrane surface area. These data imply that surface area loss in HS, but not in AIHA, is already present at the circulating reticulocyte stage. These findings imply that loss of cell surface area is an early event during genesis of HS red cells and challenge the existing concepts that surface area loss in HS occurs predominantly during the sojourn of mature red cells in circulation.
Distinctive features of “nurselike” cells that differentiate in the context of chronic lymphocytic leukemia
Authors: Tsukada Nobuhiro , Burger Jan A. , Zvaifler Nathan J. , Kipps Thomas J. 
Published: July 2002

AbstractA subset of blood mononuclear cells from patients with chronic lymphocytic leukemia (CLL) can differentiate in vitro into “nurselike” cells (NLCs) that can protect CLL cells from apoptosis. NLCs express cytoplasmic vimentin and stromal-derived factor 1 (SDF-1). NLCs also express CD14, as well as CD11b, CD33, CD40, CD45RO, CD68, CD80, CD86, HLA-DQ, and HLA-DR, but not CD1a, CD2, CD3, CD11c, CD19, CD45RA, CD83, CD106, or CD154. Consistent with this phenotype, NLCs failed to differentiate from blood mononuclear cells that were depleted of CD14+ cells or from isolated CD19+cells. CD14+ blood cells of healthy donors could differentiate into cells with the morphology and phenotype of NLCs when cultured in direct contact with CLL B cells, but not with normal B cells. Despite expressing antigens in common with blood monocytes, monocyte-derived dendritic cells, and macrophages, NLCs expressed significantly higher levels of CD68 than these other cell types. Consistent with the notion that NLCs are present in vivo, CD14+ splenocytes from CLL patients have NLC morphology and express significantly higher levels of CD68 than CD14+splenocytes from persons without known B-cell malignancy. These findings indicate that although NLCs may differentiate from blood monocytes, they probably represent a distinctive hematopoietic cell type that exists in vivo, differentiates from hematopoietic CD14+ cells in the context of CLL, and in turn protect CLL cells from apoptosis via a mechanism that is independent of CD106 (vascular cell adhesion molecule-1). The interaction between CLL cells and NLCs may represent a novel target for therapy of patients with this disease.
Functional Living Materials for Virus Disinfection Based on Engineered Bacillus subtilis Biofilm by Displaying Viral Affinity Peptides and Expressing Protease
Authors: Zhu Xiaojuan , Chen Jianshu , Luo Jian , Liu Xue , Xu Yan , Chen Lin , Dong Hao , Zhang Lingfan , Yang Lin , Jiang Nin , Huang Xumeng , Mohsin Ali , Yi Xiaoping , Wang Yonghong , Zhuang Yingping , Zhang Xuehong , Zhang Ke , Zhang Lixin , Zhong Chao , Huang Jiaofang 
Published: August 2024

AbstractGlobal pandemics caused by pathogenic viruses have highlighted the need to develop effective and sustainable materials to defend against these viruses. However, most commercial viral disinfection materials rely on precious metals, which are toxic. In this study, an engineered living material based on Bacillus subtilis biofilms is presented that can effectively remove Influenza A virus and SARS‐CoV‐2 from water. First, B. subtilis biofilm is used to display the H1N1 and H5N1 specific binding peptide C40 and SARS‐CoV‐2 specific binding peptide SBP1; thus, the biofilm materials has outstanding efficacy against H1N1, H5N1, and SARS‐CoV‐2 pseudovirus from water. In addition to capturing the virus, these biofilm materials can also inactivate the virus by secreting alkaline protease AprE, which effectively degrades the viral proteins at temperatures of 4, 16, 25, and 37 °C. Furthermore, to constrain bacterial growth and prevent the leakage of genetically modified organisms, a suicide genetic circuit using the quorum‐sensing system ComQXPA to control Lytc expression is constructed, leading to bacterial lysis. Overall, this study demonstrates a novel, effective, and sustainably engineered living material for preventing viral transmission. This material provides a safeguard for public health and promotes environmentally friendly governance.
Aggregate‐Dominated Dilute Electrolytes with Low‐Temperature‐Resistant Ion‐Conducting Channels for Highly Reversible Na Plating/Stripping
Authors: Ge Bingcheng , Deng Jiaojiao , Wang Zhijie , Liang Qinghua , Hu Liang , Ren Xiuyun , Li Runmin , Lin Yuxiao , Li Yunsong , Wang Qingrong , Han Bin , Deng Yonghong , Fan Xiulin , Li Baohua , Chen Guohua , Yu Xiaoliang 
Published: August 2024

AbstractDeveloping rechargeable batteries with high power delivery at low temperatures (LT) below 0 °C is significant for cold‐climate applications. Initial anode‐free sodium metal batteries (AFSMBs) promise high LT performances because of the low de‐solvation energy and smaller Stokes radius of Na+, nondiffusion‐limited plating/stripping electrochemistry, and maximized energy density. However, the severe reduction in electrolyte ionic conductivity and formation of unstable solid electrolyte interphase (SEI) hinder their practical applications at LT. In this study, a 2‐methyltetrahydrofuran‐based dilute electrolyte is designed to concurrently achieve an anion‐coordinated solvation structure and impressive ionic conductivity of 3.58 mS cm−1 at −40 °C. The dominant aggregate solvates enable the formation of highly efficient and LT‐resistant Na+ hopping channels in the electrolyte. Moreover, the methyl‐regulated electronic structure in 2‐methyltetrahydrofuran induces gradient decomposition toward an inorganic‐organic bilayer SEI with high Na+ mobility, composition homogeneity, and mechanical robustness. As such, a record‐high Coulombic efficiency beyond 99.9% is achieved even at −40 °C. The as‐constructed AFSMBs sustain 300 cycles with 80% capacity maintained, and a 0.5‐Ah level pouch cell delivers 85% capacity over 180 cycles at −25 °C. This study affords new insights into electrolyte formulation for fast ionic conduction and superior Na reversibility at ultralow temperatures.
Metal‐Free Carbon Co‐Catalysts for Up‐Conversion Photo‐Induced Catalytic Cancer Therapy
Authors: Wang Xichu , Sun Bing , Dai Quanbin , Zhu Lin , Gu Zi , Dai Liming 
Published: August 2024

AbstractNear‐infrared (NIR)‐responsive metal‐free carbon co‐catalysts that convert glucose into H2O2 to generate reactive oxygen species (ROS) are developed from phosphorus‐doped carbon nitride (P‐C3N4) and graphene quantum dots (GQD) composites, for enhanced photocatalytic cancer therapy by light exposure in the targeted tumor microenvironment. Upon irradiation, the NIR light is converted by GQD with up‐conversion function into visible light to excite P‐C3N4 for photocatalytic conversion of glucose into H2O2, which subsequently decomposes into ROS. ROS thus generated exhibits an excellent anticancer efficacy for efficient cancer therapy with minimal side effects, as evidenced by both in vitro and in vivo studies. This study demonstrates, for the first time, a cancer therapeutic of GQD/P‐C3N4 composite that utilizes a two‐step cascade effect using initially NIR‐triggered GQD nanoparticles to activate P‐C3N4 to photocatalytically generate ROS for effective and targeted cancer therapy.
Liquid Metal Hydrothermal Rheological Modification Method for High Performance Gallium‐Coated Carbon Microparticle Composites
Authors: Zhou Xiao‐Ping , Luo Zheng , Yang Dong‐Xu 
Published: August 2024

AbstractGallium‐based liquid metal (LM) is widely used in flexible electronics, optics, and green synthesis due to its excellent conductivity, flexibility, and self‐healing capabilities. However, LM's inherent fluidity and high surface tension greatly limit their practical applications. Therefore, there is a strong demand for developing LM composites that are easy to control and exhibit outstanding performance when used flexibly. In this work, the LM hydrothermal rheological modification method is proposed to synthesize Ga‐coated carbon microparticles, and the highly miscible rheological modification of LM is realized by mixing LM and Ga‐coated carbon microparticles. Including carbon microparticles in the LM improves the mechanical strength of the composite, thereby overcoming the limitation of the LM that has a low mechanical strength. By controlling the volume fraction of carbon microparticles in LM, electrical conductivity is increased by 30% and thermal conductivity by more than 2.0 times that of pure LM. In addition, the fundamental interfacial wetting behavior is demonstrated at the interface of LM and Ga2O3, and the rheological modification mechanism of LM is explained by carbon particles. This work presents a novel method for preparing high‐performance polymer materials and discusses their broad potential applications in thermal interface materials, wireless energy transfer, and flexible electronics.
Cation‐Doped Cs2BIBIIIX6 Double Perovskites: Electronic Structures, Optical Properties, and Optoelectronic Applications
Authors: Jiang Jialiang , Du Zhentao , Fu Hui , Li Wenjing , Zhang Hao , Xie Hui , Zheng Jinju , Yang Weiyou 
Published: August 2024

AbstractRecently, all‐inorganic halide double perovskites (DPs) with a chemical formula of Cs2BIBIIIX6 have been emerging as a shinning star to be used in modern optoelectronic devices, due to their unique advantages such as environmentally friendly, high absorption coefficient, low trap densities, tunable bandgap, variable constituent elements and valences states through BI or BIII sites engineering. In this review, first, the impact of BI and BIII cation doping on the electronic structure and optical properties of Cs2BIBIIIX6 DPs are systematically overviewed, which cover several key issues including the interconversion between direct and indirect band gaps, the enhancement of light absorption, promotion of exciton localization, elucidation of energy transfer mechanisms, regulation of defect density and improvement in both stability and luminescence efficiency. Subsequently, state‐of‐the‐art research activities regarding their interesting optoelectronic device applications are systematically discussed, such as light‐emitting diodes, anticounterfeiting encryption, X‐ray scintillators, and optical thermometry. Finally, the existing difficulties, prospects, and research directions in this field are proposed.
Irreversible Electroporation has More Synergistic Effect with Anti‐PD‐1 Immunotherapy than Thermal Ablation or Cryoablation, in a Colorectal Cancer Model
Authors: Jiang Minhan , Shao Qi , Slaughter Joseph , Bischof John 
Published: August 2024

AbstractBoosting the response rate of immune checkpoint blockade (ICB) therapy to improve treatment efficacy is a primary goal in cancer immunotherapy. One of the promising approaches involves focal tumor ablation to reduce tumor burden and trigger the in situ vaccination. Even though this combination strategy has demonstrated enhanced therapeutic outcomes in both preclinical research and clinical trials, limited research has comparatively investigated diverse ablation techniques. The optimal choice among focal therapy techniques remains largely unknown. In a murine colorectal cancer model (MC‐38), the therapeutic efficacy of anti‐PD‐1 in combination with thermal ablation, cryoablation, and irreversible electroporation (IRE) is evaluated, utilizing well‐characterized miniature probes. In this model, ICB monotherapy has limited effect in controlling tumor growth. IRE exhibits the most favorable synergistic effect with anti‐PD‐1 immunotherapy than thermal ablation or cryoablation, leading to the greatest primary tumor growth delay, longest tumor‐free survival, and highest protection against secondary tumor challenge. Furthermore, the co‐administration of IRE and anti‐PD‐1 significantly fosters the infiltration of CD8+ T cells into the tumor coupled with a remarkable stem‐like progenitor phenotype. The findings demonstrate that IRE stands as a promising modality that can potentiate the antitumor efficacy when the tumor is poorly responding to ICB monotherapy.
Mitochondria Transplantation to Bone Marrow Stromal Cells Promotes Angiogenesis During Bone Repair
Authors: Wang Yifan , Li Wenjing , Guo Yusi , Huang Ying , Guo Yaru , Song Jia , Mei Feng , Liao Peiwen , Gong Zijian , Chi Xiaopei , Deng Xuliang 
Published: August 2024

AbstractAngiogenesis is crucial for successful bone defect repair. Co‐transplanting Bone Marrow Stromal Cells (BMSCs) and Endothelial Cells (ECs) has shown promise for vascular augmentation, but it face challenges in hostile tissue microenvironments, including poor cell survival and limited efficacy. In this study, the mitochondria of human BMSCs are isolated and transplanted to BMSCs from the same batch and passage number (BMSCsmito). The transplanted mitochondria significantly boosted the ability of BMSCsmito‐ECs to promote angiogenesis, as assessed by in vitro tube formation and spheroid sprouting assays, as well as in vivo transplantation experiments in balb/c mouse and SD rat models. The Dll4‐Notch1 signaling pathway is found to play a key role in BMSCsmito‐induced endothelial tube formation. Co‐transplanting BMSCsmito with ECs in a rat cranial bone defect significantly improves functional vascular network formation, and improve bone repair outcomes. These findings thus highlight that mitochondrial transplantation, by acting through the DLL4‐Notch1 signaling pathway, represents a promising therapeutic strategy for enhancing angiogenesis and improving bone repair. Hence, mitochondrial transplantation to BMSCS as a therapeutic approach for promoting angiogenesis offers valuable insights and holds much promise for innovative regenerative medicine therapies.
Piezoelectric Bilayer Nickel‐Iron Layered Double Hydroxide Nanosheets with Tumor Microenvironment Responsiveness for Intensive Piezocatalytic Therapy
Authors: Liu Shaohua , Bao Jianchun , Tian Boshi , Li Shuyao , Yang Meiqi , Yang Dan , Lu Xuyun , Liu Xueliang , Gai Shili , Yang Piaoping 
Published: August 2024

AbstractPiezocatalytic therapy (PCT) based on 2D layered materials has emerged as a promising non‐invasive tumor treatment modality, offering superior advantages. However, a systematic investigation of PCT, particularly the mechanisms underlying the reactive oxygen species (ROS) generation by 2D nanomaterials, is still in its infancy. Here, for the first time, biodegradable piezoelectric 2D bilayer nickel‐iron layered double hydroxide (NiFe‐LDH) nanosheets (thickness of ≈1.86 nm) are reported for enhanced PCT and ferroptosis. Under ultrasound irradiation, the piezoelectric semiconducting NiFe‐LDH exhibits a remarkable ability to generate superoxide anion radicals, due to the formation of a built‐in electric field that facilitates the separation of electrons and holes. Notably, the significant excitonic effect in the ultrathin NiFe‐LDH system enables long‐lived excited triplet excitons (lifetime of ≈5.04 µs) to effectively convert triplet O2 molecules into singlet oxygen. Moreover, NiFe‐LDH exhibited tumor microenvironment (TME)‐responsive peroxidase (POD)‐like and glutathione (GSH)‐depleting capabilities, further enhancing oxidative stress in tumor cells and inducing ferroptosis. To the best of knowledge, this is the first report on piezoelectric semiconducting sonosensitizers based on LDHs for PCT and ferroptosis, providing a comprehensive understanding of the piezocatalysis mechanism and valuable references for the application of LDHs and other 2D materials in cancer therapy.
Bacterial Iron Siderophore Drives Tumor Survival and Ferroptosis Resistance in a Biofilm‐Tumor Spheroid Coculture Model
Authors: Yeung Yoyo Wing Suet , Ma Yeping , Deng Yanlin , Khoo Bee Luan , Chua Song Lin 
Published: August 2024

AbstractInteractions between tumoral cells and tumor‐associated bacteria within the tumor microenvironment play a significant role in tumor survival and progression, potentially impacting cancer treatment outcomes. In lung cancer patients, the Gram‐negative pathogen Pseudomonas aeruginosa raises questions about its role in tumor survival. Here, a microfluidic‐based 3D‐human lung tumor spheroid‐P. aeruginosa model is developed to study the bacteria's impact on tumor survival. P. aeruginosa forms a tumor‐associated biofilm by producing Psl exopolysaccharide and secreting iron‐scavenging pyoverdine, which is critical for establishing a bacterial community in tumors. Consequently, pyoverdine promotes cancer progression by reducing susceptibility to iron‐induced death (ferroptosis), enhancing cell viability, and facilitating several cancer hallmarks, including epithelial–mesenchymal transition and metastasis. A promising combinatorial therapy approach using antimicrobial tobramycin, ferroptosis‐inducing thiostrepton, and anti‐cancer doxorubicin could eradicate biofilms and tumors. This work unveils a novel phenomenon of cross‐kingdom cooperation, where bacteria protect tumors from death, and it paves the way for future research in developing antibiofilm cancer therapies. Understanding these interactions offers potential new strategies for combatting cancer and enhancing treatment efficacy.
Berberine Protects against Hepatocellular Carcinoma Progression by Regulating Intrahepatic T Cell Heterogeneity
Authors: Hu Jiaxiang , Shi Qingmiao , Xue Chen , Wang Qingqing 
Published: August 2024

AbstractAccumulating evidence suggests that berberine (BBR) exhibits anti‐cancer effects in hepatocellular carcinoma (HCC). However, the mechanisms by which BBR regulates the immunological microenvironment in HCC has not been fully elucidated. In this study, a mouse model of orthotopic HCC is established and treated with varying doses of BBR. BBR showed effectiveness in reducing tumor burden in mice with HCC. Cytometry by time‐of‐flight depicted the alterations in the tumor immune landscape following BBR treatment, revealing the enhancement in the T lymphocytes effector function. In particular, BBR decreased the proportion of TCRbhiPD‐1hiCD69+CD27+ effector CD8+ T lymphocytes and increased the proportion of Ly6ChiTCRb+CD69+CD27+CD62L+ central memory CD8+ T lymphocytes. Single‐cell RNA sequencing further elucidates the effects of BBR on transcriptional profiles of liver immune cells and confirms the phenotypical heterogeneity of T lymphocytes in HCC immune microenvironment. Additionally, it is found that BBR potentially regulated the antitumor immunity in HCC by modulating the receptor‐ligand interaction among immune cells mediated by cytokines. In summary, the findings improve the understanding of BBR's impact on protecting against HCC, emphasizing BBR's role in regulating intrahepatic T cell heterogeneity. BBR has the potential to be a promising therapeutic strategy to hinder the advancement of HCC.