bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three‐dimensional transperineal ultrasound for imaging mesh implants following sacrocolpopexy
Authors: Eisenberg V. H. , Steinberg M. , Weiner Z. , Alcalay M. , Itskovitz‐Eldor J. , Schiff E. , Lowenstein L. 
Published: January 2014

ABSTRACTObjectiveTo characterize, using three‐dimensional (3D) transperineal ultrasound, the appearance, position and dimensions of mesh implants following minimally invasive abdominal sacrocolpopexy.MethodsIn women who underwent sacrocolpopexy, mesh was evaluated at rest and on maximal Valsalva, on all 3D orthogonal planes and rendered views. Mesh dimensions were obtained by 3D processing in the midsagittal and coronal planes (anterior, posterior and sacral arm) and were analyzed offline, the operator blinded to clinical data.ResultsOverall, 62 women, mean age 58.4 (range, 42–79) years were evaluated at a median of 9 (range, 1–26) months following surgery. The anterior arm of the mesh was caudal to the lowermost point of descent of the anterior compartment in 56 (90.3%) women, was equally positioned in five (8.1%) and was cranial in one. The posterior arm was caudal in 44 (71%) women, was equally positioned in 16 (25.8%) and was cranial in two (3.2%). The Y connection and the sacral arm of the mesh could not be adequately seen because of physical limitations of ultrasound (lower resolution at greater depth), large recurrent rectoceles, echogenic stools or folding of mesh remnants. Folding of the mesh was seen in 46 (74.2%) women, folding of the anterior arm in five (8.1%) and folding of the posterior arm in 23 (37.1%). Folding occurred caudally in 26 (41.9%) women, proximally in 11 (17.7%) and in both areas in nine (14.5%). There were no erosions.ConclusionMesh visualization following minimally invasive abdominal sacrocolpopexy procedures using transperineal 3D/four‐dimensional (4D) ultrasound is feasible. Studies are needed to evaluate the correlation between ultrasound measures and prolapse recurrence or mesh erosion. Copyright © 2014 ISUOG. Published by John Wiley & Sons Ltd.
Seasonal variations in PCR‐DGGE fingerprinted viruses infecting phytoplankton in large and deep peri‐alpine lakes
Authors: Zhong X. , Rimet F. , Jacquet S. 
Published: January 2014

AbstractDouble‐stranded DNA viruses infecting eukaryotic algae (e.g., phycodnaviruses) and cyanobacteria (e.g., cyanophages) are now recognized as widespread and ubiquitous in aquatic environments. However, both the diversity and functional roles of these viruses in fresh waters are still poorly understood. We conducted a year‐long study in 2011 of the community structure of planktonic virus groups in the upper lit layer of two important freshwater natural ecosystems in France, Lake Annecy (oligotrophic) and Lake Bourget (oligo‐mesotrophic). Using PCR‐DGGE to target a number of different structural and functional signature genes, i.e., g20, g23, psbA, polB, and mcp, the phytoplankton viruses were shown to display temporal and spatial variability. There were marked seasonal changes in community structure for all viral groups in Lake Bourget, but only for T4‐like myoviruses and psbA‐containing cyanophages in Lake Annecy. The multivariate statistical analyses revealed that (1) various environmental factors can directly or indirectly explain the community structure observed for each phytoplankton viral group, and (2) temporal patterns of T4‐like myovirus community structure were similar between the two lakes. In general, our results (1) suggest that the observed algal virus patterns were associated with significant shifts in phytoplankton biomass and/or structure, which in turn were shaped by the abiotic environment, and (2) support the Bank model proposed by Breitbart and Rohwer (Trends Microbiol 13:278–284, 2005). This study provides new evidence that freshwater lakes contain a significant diversity of algal viruses, and that the distribution of these viruses strongly mirrors that of their hosts.
Retrofitting social housing: reflections by tenants on adopting and living with retrofit technology
Authors: Brown Philip , Swan Will , Chahal Sharon 
Published: January 2014

Abstract
Retrofit has been described as one of the major engineering challenges of the twenty-first century (Kelly 2009). However, the industry needs to look beyond regarding the problem as restricted to the physical upgrade of properties. Asset managers, engineers and installers work on and in people’s homes and, in many cases, are subsequently changing the way householders use their homes to meet their comfort and wider energy needs. Here we consider how the twin issues of adopting and living with retrofit technologies have affected groups of residents in social housing. We discuss issues of trust, social norms, engagement and concern that have shaped the adoption process, as well as investigating the everyday experience of living with new configurations of energy consumption. The findings have relevance not only for the social housing sector but also raise questions as to how to effectively deliver programmes such as the Green Deal and the Energy Company Obligation within the UK.
Cryptic diversity in host-associated populations ofTetra pinnatifidae(Acari: Eriophyoidea): What do morphometric, mitochondrial and nuclear data reveal and conceal?
Authors: Li Hao-Sen , Xue Xiao-Feng , Hong Xiao-Yue 
Published: January 2014

AbstractTraditional morphology-based taxonomy of eriophyoid mites (Acari: Eriophyoidea) has been challenged by molecular-based technologies in the detection of cryptic species. However, the implications of such cryptic diversity appear to differ when methods based on different types of data are used. Here, samples of a host-associated eriophyoid mite species,Tetra pinnatifidae, collected from different host plants and localities are evaluated. The congruence of results based on morphometric (32 characters), mitochondrial (16S), and nuclear (28S) data were evaluated and showed a host-associated cryptic diversity dividing this morphospecies into several groups/clades that were morphometrically indistinguishable. In comparison, the 16S data confirmed cryptic speciation and intra-clade host-associated diversity, while 28S did not. In contrast, 28S data revealed potential gene flow between host-associated populations. High mitochondrial divergence, as well as low nuclear and morphological divergence indicated very recent stage of cryptic diversity of this eriophyoid mite.
The effects of circulating testosterone and pubertal maturation on risk for disordered eating symptoms in adolescent males
Authors: Culbert K. M. , Burt S. A. , Sisk C. L. , Nigg J. T. , Klump K. L. 
Published: January 2014

BackgroundTestosterone may be a biological factor that protects males against eating disorders. Elevated prenatal testosterone exposure is linked to lower levels of disordered eating symptoms, but effects emerge only after mid-puberty. Whether circulating levels of testosterone account for decreased risk for disordered eating in boys after mid-puberty is currently unknown; however, animal data support this possibility. In rodents, prenatal testosterone's masculinizing effects on sex-differentiated behaviors emerge during puberty when circulating levels of testosterone increase and ‘activate’ the expression of masculinized phenotypes. This study investigated whether higher levels of circulating testosterone predict lower levels of disordered eating symptoms in adolescent boys, and in particular whether effects are associated with advancing pubertal maturation.MethodParticipants were 213 male twins from the Michigan State University Twin Registry. The Minnesota Eating Behavior Survey and Eating Disorder Examination Questionnaire assessed several disordered eating symptoms. The Pubertal Development Scale assessed pubertal status. Afternoon saliva samples were assayed for testosterone using enzyme immunoassays.ResultsConsistent with animal data, higher levels of circulating testosterone predicted lower levels of disordered eating symptoms in adolescent boys and effects emerged with advancing puberty. Results were not accounted for by several important covariates, including age, adiposity, or mood/anxiety symptoms.ConclusionsFindings suggest that elevated circulating testosterone may be protective and underlie decreased risk for eating pathology in males during/after puberty, whereas lower levels of testosterone may increase risk and explain why some, albeit relatively few, males develop eating disorders.
Hearing punk as blues
Authors: Rapport Evan 
Published: January 2014

AbstractPunk is an extreme manifestation of the rock project, meaning that it depends on an association of raw and authentic expression with blackness, musically represented by particular approaches to the blues, combined with processes of white appropriation, transformation and obfuscation of those blues resources. Punk's powerful affect largely derives from the tension between punk's blues foundations and strategies that obscure its roots. Punk treatments and transformations of the blues include: (1) moving from multi-part, three-chord harmonic schemes to riffs and one- and two-chord vamps; (2) taking a new approach to vocal performance styles; and (3) retaining certain melodic approaches, such as the use of pentatonic scales and monophonic textures. Punk's complex relationship to the blues, including the history of negation and erasure, continues to be a central part of the punk aesthetic.
End-organ consequences of the Fontan operation: liver fibrosis, protein-losing enteropathy and plastic bronchitis
Authors: Rychik Jack , Goldberg David , Rand Elizabeth , Semeao Edisio , Russo Pierre , Dori Yoav , Dodds Kathryn 
Published: January 2014

AbstractThe Fontan operation, although part of a life-saving surgical strategy, manifests a variety of end-organ complications and unique morbidities that are being recognised with increasing frequency as patients survive into their second and third decades of life and beyond. Liver fibrosis, protein-losing enteropathy and plastic bronchitis are consequences of a complex physiology involving circulatory insufficiency, inflammation and lymphatic derangement. These conditions are manifest in a chronic, indolent state. Management strategies are emerging, which shed some light on the origins of these complications. A better characterisation of the end-organ consequences of the Fontan circulation is necessary, which can then allow for development of specific methods for treatment. Ideally, the goal is to establish systematic strategies that might reduce or eliminate the development of these potentially life-threatening challenges.
Tetralogy of Fallot: nosological, morphological, and morphogenetic considerations
Authors: Anderson Robert H. , Spicer Diane E. , Giroud Jorge M. , Mohun Timothy J. 
Published: January 2014

AbstractIt is timely, in the 125th anniversary of the initial description by Fallot of the hearts most frequently seen in patients presenting with “la maladie bleu”, that we revisit his descriptions, and discuss his findings in the light of ongoing controversies. Fallot described three hearts in his initial publication, and pointed to the same tetralogy of morphological features that we recognise today, namely, an interventricular communication, biventricular connection of the aorta, subpulmonary stenosis, and right ventricular hypertrophy. In one of the hearts, he noted that the aorta arose exclusively from the right ventricle. In other words, one of his initial cases exhibited double-outlet right ventricle. When we now compare findings in hearts with the features of the tetralogy, we can observe significant variations in the nature of the borders of the plane of deficient ventricular septation when viewed from the aspect of the right ventricle. We also find that this plane, usually described as the ventricular septal defect, is not the same as the geometric plane separating the cavities of the right and left ventricles. This means that the latter plane, the interventricular communication, is not necessarily the same as the ventricular septal defect. We are now able to provide further insights into these features by examining hearts prepared from developing mice. Additional molecular investigations will be required, however, to uncover the mechanisms responsible for producing the morphological changes underscoring tetralogy of Fallot.
Surgical pulmonary valve insertion
Authors: Lee Cheul , Lee Chang-Ha , Kwak Jae Gun 
Published: January 2014

AbstractPulmonary valve replacement is being performed with increasing frequency in patients with various congenital heart diseases. Chronic pulmonary regurgitation after repair of tetralogy of Fallot is a typical situation that requires pulmonary valve replacement. Chronic pulmonary regurgitation after repair of tetralogy of Fallot can lead to right ventricular dilatation, biventricular dysfunction, heart failure symptoms, arrhythmias, and sudden death. Although pulmonary valve replacement can lead to improvement in functional class and a substantial decrease or normalisation of right ventricular volumes, the optimal timing of pulmonary valve replacement in patients with chronic pulmonary regurgitation is still unknown. There are several options for surgical pulmonary valve replacement. However, no ideal pulmonary valve substitute exists currently and most of the surgically implanted pulmonary valves will eventually require re-replacement. This article will review options and timing of surgical pulmonary valve insertion in patients with chronic pulmonary regurgitation after repair of tetralogy of Fallot.
The importance of small for gestational age in the risk assessment of infants with critical congenital heart disease
Authors: Sochet Anthony A. , Ayers Mark , Quezada Emilio , Braley Katherine , Leshko Jennifer , Amankwah Ernest K. , Quintessenza James A. , Jacobs Jeffrey P. , Dadlani Gul 
Published: January 2014

AbstractBackgroundInfants with critical congenital heart disease who require cardiothoracic surgical intervention may have significant post-operative mortality and morbidity. Infants who are small for gestational age <10th percentile with foetal growth restriction may have end-organ dysfunction that may predispose them to increased morbidity or mortality.MethodsA single-institution retrospective review was performed in 230 infants with congenital heart disease who had cardiothoracic surgical intervention <60 days of age. Pre-, peri-, and post-operative morbidity and mortality markers were collected along with demographics and anthropometric measurements.ResultsThere were 230 infants, 57 (23.3%) small for gestational age and 173 (70.6%) appropriate for gestational age. No significant difference was noted in pre-operative markers – gestational age, age at surgery, corrected gestational age, Society for Thoracic Surgeons and European Association for Cardiothoracic Surgery mortality score; or post-operative factors – length of stay, ventilation days, arrhythmias, need for extracorporeal membrane oxygenation, vocal cord dysfunction, hearing loss; or end-organ dysfunction – gastro-intestinal, renal, central nervous system, or genetic. Small for gestational age infants were more likely to have failed vision tests (p = 0.006). Small for gestational age infants were more likely to have increased 30-day (p = 0.005) and discharge mortality (p = 0.035). Small for gestational age infants with normal birth weight (>2500 g) were also at increased risk of 30-day mortality compared with appropriate for gestational age infants (p = 0.045).ConclusionsSmall for gestational age infants with congenital heart disease who undergo cardiothoracic surgery <60 days of age have increased risk of mortality and failed vision screening. Assessment of foetal growth restriction as part of routine pre-operative screening may be beneficial.
Host-specificity among abundant and rare taxa in the sponge microbiome
Authors: Reveillaud Julie , Maignien Loïs , Eren A Murat , Huber Julie A , Apprill Amy , Sogin Mitchell L , Vanreusel Ann 
Published: January 2014

Abstract
Microbial communities have a key role in the physiology of the sponge host, and it is therefore essential to understand the stability and specificity of sponge–symbiont associations. Host-specific bacterial associations spanning large geographic distance are widely acknowledged in sponges. However, the full spectrum of specificity remains unclear. In particular, it is not known whether closely related sponges host similar or very different microbiota over wide bathymetric and geographic gradients, and whether specific associations extend to the rare members of the sponge microbiome. Using the ultra-deep Illumina sequencing technology, we conducted a comparison of sponge bacterial communities in seven closely related Hexadella species with a well-resolved host phylogeny, as well as of a distantly related sponge Mycale. These samples spanned unprecedentedly large bathymetric (15–960 m) gradients and varying European locations. In addition, this study included a bacterial community analysis of the local background seawater for both Mycale and the widespread deep-sea taxa Hexadella cf. dedritifera. We observed a striking diversity of microbes associated with the sponges, spanning 47 bacterial phyla. The data did not reveal any Hexadella microbiota co-speciation pattern, but confirmed sponge-specific and species-specific host–bacteria associations, even within extremely low abundant taxa. Oligotyping analysis also revealed differential enrichment preferences of closely related Nitrospira members in closely related sponges species. Overall, these results demonstrate highly diverse, remarkably specific and stable sponge–bacteria associations that extend to members of the rare biosphere at a very fine phylogenetic scale, over significant geographic and bathymetric gradients.
Delineating cooperative responses of processive motors in living cells
Authors: Efremov Artem K. , Radhakrishnan Anand , Tsao David S. , Bookwalter Carol S. , Trybus Kathleen M. , Diehl Michael R. 
Published: January 2014

Significance
Although many vesicles and organelles are known to be transported by groups of interacting cytoskeletal motors, the precise impact of collective motor behaviors on intracellular transport and trafficking processes remains controversial. By engineering COS-7 cells to provide genetic control over the density of motors on, and the sizes of vesicles (peroxisomes), we performed systematic comparisons of the collective behaviors of kinesin and myosinVa motors. The responses of cargo velocities, run lengths, and position fluctuations to these parameters suggest that myosinVa motors can cooperate more productively than kinesins when transporting cargos as a team. This behavior is derived from the mechanochemical properties of these motors and suggests that the collective functions of motors like myosinV can be regulated more sensitively than those of kinesin.
Prediction of therapeutic microRNA based on the human metabolic network
Authors: Wu Ming , Chan Christina 
Published: January 2014

Abstract
Motivation: MicroRNA (miRNA) expression has been found to be deregulated in human cancer, contributing, in part, to the interest of the research community in using miRNAs as alternative therapeutic targets. Although miRNAs could be potential targets, identifying which miRNAs to target for a particular type of cancer has been difficult due to the limited knowledge on their regulatory roles in cancer. We address this challenge by integrating miRNA-target prediction, metabolic modeling and context-specific gene expression data to predict therapeutic miRNAs that could reduce the growth of cancer.
Results: We developed a novel approach to simulate a condition-specific metabolic system for human hepatocellular carcinoma (HCC) wherein overexpression of each miRNA was simulated to predict their ability to reduce cancer cell growth. Our approach achieved &gt;80% accuracy in predicting the miRNAs that could suppress metastasis and progression of liver cancer based on various experimental evidences in the literature. This condition-specific metabolic system provides a framework to explore the mechanisms by which miRNAs modulate metabolic functions to affect cancer growth. To the best of our knowledge, this is the first computational approach implemented to predict therapeutic miRNAs for human cancer based on their functional role in cancer metabolism. Analyzing the metabolic functions altered by the miRNA-identified metabolic genes essential for cell growth and proliferation that are targeted by the miRNAs.
Availability and implementation: See supplementary protocols and http://www.egr.msu.edu/changroup/Protocols%20Index.html.
Contact: krischan@egr.msu.edu
Supplementary information: Supplementary data are available at Bioinformatics online.
Randomized double‐blind study of the effect of dexamethasone and methylprednisolone pulse in the control of rheumatoid arthritis flare‐up: a preliminary study
Authors: Sadra Vahideh , Khabbazi Alireza , Kolahi Susan , Hajialiloo Mehrzad , Ghojazadeh Morteza 
Published: January 2014

AbstractAimThe objective of this study was to compare the efficacy and safety of dexamethasone and methylprednisolone for pulse therapy of rheumatoid arthritis flare‐up.MethodsThis randomized double‐blind controlled study was performed in the Emam Reza Educational Hospital of Tabriz University of Medical Sciences, Tabriz, Iran. Thirty rheumatoid arthritis patients who had severely active disease were recruited to the dexamethasone and methylprednisolone pulse groups. Disease activity of all the patients was measured by the Disease Activity Score in 28 joints (DAS28) at baseline, and days 4 and 30.ResultsThe differences in the DAS28 at days 4 and 30, and the number of patients whose DAS28 obtained less than 3.2 and 2.6 in the dexamethasone and methylprednisolone groups were non‐significant. There was not any significant difference between the adverse effects of the treatments in the two groups.ConclusionsThe results of the study suggest that dexamethasone pulse therapy is a safe and effective treatment for severe rheumatoid arthritis flare‐up.
Prospective phase II study of cisplatin plus pemetrexed with maintenance of pemetrexed for advanced non‐squamous cell non‐small cell lung cancer in Japan
Authors: Asami Kazuhiro , Kawahara Masaaki , Hirashima Tomonori , Suzuki Hidekazu , Okishio Kyoichi , Omachi Naoki , Tamiya Motohiro , Tamiya Akihiro , Hirooka Aya , Nakao Keiko , Tsuji Taisuke , Atagi Shinji 
Published: January 2014

AbstractBackgroundA previous study showed a survival benefit with maintenance therapy with pemetrexed in patients with advanced non‐small cell lung cancer (NSCLC). However, it remains unclear whether continuation maintenance therapy with pemetrexed is beneficial in Japanese patients. Here, we present our phase II study that assessed the efficacy and safety of cisplatin plus pemetrexed as induction chemotherapy, followed by maintenance therapy with pemetrexed in advanced NSCLC patients in Japan.MethodsChemotherapy‐naïve patients received 500 mg/m2 pemetrexed and 75 mg/m2 cisplatin on day one every three weeks for four cycles. In patients who responded to therapy or achieved stable disease, pemetrexed was continued until disease progression. The primary endpoint of this study was the progression‐free survival rate at six months (PFS‐6).ResultsOf the 35 patients initially enrolled in the study, 18 (51%) received maintenance chemotherapy with pemetrexed. The median PFS was 6.7 months, and the PFS‐6 was 60% (95% confidence interval [CI], 42–76%). Median overall survival (OS) was 15.5 months (95% CI, 8.3–22.7 months). The median PFS and OS in patients who received maintenance chemotherapy with pemetrexed were 9.5 months and 25.3 months, respectively. The most frequently noted severe toxicity during induction chemotherapy was neutropenia, which occurred in seven patients. Two patients discontinued maintenance therapy owing to prolonged grade 2 edema in one patient and grade 3 neutropenia in another.ConclusionContinuation maintenance chemotherapy with pemetrexed is associated with a survival benefit in patients who have completed induction chemotherapy for non‐squamous NSCLC.