bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Graph Theoretical Approach to Data Fusion
Authors: Žurauskienė Justina , Kirk Paul DW , Stumpf Michael PH 
Published: August 2015

ABSTRACTThe rapid development of high throughput experimental techniques has resulted in a growing diversity of genomic datasets being produced and requiring analysis. A variety of computational techniques allow us to analyse such data and to model the biological processes behind them. However, it is increasingly being recognised that we can gain deeper understanding by combining the insights obtained from multiple, diverse datasets. We therefore require scalable computational approaches for data fusion.We propose a novel methodology for scalable unsupervised data fusion. Our technique exploits network representations of the data in order to identify (and quantify) similarities among the datasets. We may work within the Bayesian formalism, using Bayesian nonparametric approaches to model each dataset; or (for fast, approximate, and massive scale data fusion) can naturally switch to more heuristic modelling techniques. An advantage of the proposed approach is that each dataset can initially be modelled independently (and therefore in parallel), before applying a fast post-processing step in order to perform data fusion. This allows us to incorporate new experimental data in an online fashion, without having to rerun all of the analysis. The methodology can be applied to genomic scale datasets and we demonstrate its applicability on examples from the literature, using a broad range of genomic datasets, and also on a recent gene expression dataset fromSporadic inclusion body myositis Availability. Example R code and instructions are available fromhttps://sites.google.com/site/gtadatafusion/.
Overexpression of BAX INHIBITOR-1 Links Plasma Membrane Microdomain Proteins to Stress
Authors: Ishikawa Toshiki , Aki Toshihiko , Yanagisawa Shuichi , Uchimiya Hirofumi , Kawai-Yamada Maki 
Published: August 2015

AbstractBAX INHIBITOR-1 (BI-1) is a cell death suppressor widely conserved in plants and animals. Overexpression of BI-1 enhances tolerance to stress-induced cell death in plant cells, although the molecular mechanism behind this enhancement is unclear. We recently found that Arabidopsis (Arabidopsis thaliana) BI-1 is involved in the metabolism of sphingolipids, such as the synthesis of 2-hydroxy fatty acids, suggesting the involvement of sphingolipids in the cell death regulatory mechanism downstream of BI-1. Here, we show that BI-1 affects cell death-associated components localized in sphingolipid-enriched microdomains of the plasma membrane in rice (Oryza sativa) cells. The amount of 2-hydroxy fatty acid-containing glucosylceramide increased in the detergent-resistant membrane (DRM; a biochemical counterpart of plasma membrane microdomains) fraction obtained from BI-1-overexpressing rice cells. Comparative proteomics analysis showed quantitative changes of DRM proteins in BI-1-overexpressing cells. In particular, the protein abundance of FLOTILLIN HOMOLOG (FLOT) and HYPERSENSITIVE-INDUCED REACTION PROTEIN3 (HIR3) markedly decreased in DRM of BI-1-overexpressing cells. Loss-of-function analysis demonstrated that FLOT and HIR3 are required for cell death by oxidative stress and salicylic acid, suggesting that the decreased levels of these proteins directly contribute to the stress-tolerant phenotypes in BI-1-overexpressing rice cells. These findings provide a novel biological implication of plant membrane microdomains in stress-induced cell death, which is negatively modulated by BI-1 overexpression via decreasing the abundance of a set of key proteins involved in cell death.
Isolation and Characterization of O-methyltransferases Involved in the Biosynthesis of Glaucine in Glaucium flavum
Authors: Chang Limei , Hagel Jillian M. , Facchini Peter J. 
Published: August 2015

Abstract
Transcriptome resources for the medicinal plant Glaucium flavum were searched for orthologs showing identity with characterized O-methyltransferases (OMTs) involved in benzylisoquinoline alkaloid biosynthesis. Seven recombinant proteins were functionally tested using the signature alkaloid substrates for six OMTs: norlaudanosoline 6-OMT, 6-O-methyllaudanosoline 4′-OMT, reticuline 7-OMT, norreticuline 7-OMT, scoulerine 9-OMT, and tetrahydrocolumbamine OMT. A notable alkaloid in yellow horned poppy (G. flavum [GFL]) is the aporphine alkaloid glaucine, which displays C8-C6′ coupling and four O-methyl groups at C6, C7, C3′, and C4′ as numbered on the 1-benzylisoquinoline scaffold. Three recombinant enzymes accepted 1-benzylisoquinolines with differential substrate and regiospecificity. GFLOMT2 displayed the highest amino acid sequence identity with norlaudanosoline 6-OMT, showed a preference for the 6-O-methylation of norlaudanosoline, and O-methylated the 3′ and 4′ hydroxyl groups of certain alkaloids. GFLOMT1 showed the highest sequence identity with 6-O-methyllaudanosoline 4′OMT and catalyzed the 6-O-methylation of norlaudanosoline, but more efficiently 4′-O-methylated the GFLOMT2 reaction product 6-O-methylnorlaudanosoline and its N-methylated derivative 6-O-methyllaudanosoline. GFLOMT1 also effectively 3′-O-methylated both reticuline and norreticuline. GFLOMT6 was most similar to scoulerine 9-OMT and efficiently catalyzed both 3′- and 7′-O-methylations of several 1-benzylisoquinolines, with a preference for N-methylated substrates. All active enzymes accepted scoulerine and tetrahydrocolumbamine. Exogenous norlaudanosoline was converted to tetra-O-methylated laudanosine using combinations of Escherichia coli producing (1) GFLOMT1, (2) either GFLOMT2 or GFLOMT6, and (3) coclaurine N-methyltransferase from Coptis japonica. Expression profiles of GFLOMT1, GFLOMT2, and GFLOMT6 in different plant organs were in agreement with the O-methylation patterns of alkaloids in G. flavum determined by high-resolution, Fourier-transform mass spectrometry.
The RING-Finger Ubiquitin Ligase HAF1 Mediates Heading date 1 Degradation during Photoperiodic Flowering in Rice
Authors: Yang Ying , Fu Debao , Zhu Chunmei , He Yizhou , Zhang Huijun , Liu Tao , Li Xianghua , Wu Changyin 
Published: August 2015

Abstract
The photoperiodic response is one of the most important factors determining heading date in rice (Oryza sativa). Although rhythmic expression patterns of flowering time genes have been reported to fine-tune the photoperiodic response, posttranslational regulation of key flowering regulators has seldom been elucidated in rice. Heading date 1 (Hd1) encodes a zinc finger transcription factor that plays a crucial role in the photoperiodic response, which determines rice regional adaptability. However, little is known about the molecular mechanisms of Hd1 accumulation during the photoperiod response. Here, we identify a C3HC4 RING domain-containing E3 ubiquitin ligase, Heading date Associated Factor 1 (HAF1), which physically interacts with Hd1. HAF1 mediates ubiquitination and targets Hd1 for degradation via the 26S proteasome-dependent pathway. The haf1 mutant exhibits a later flowering heading date under both short-day and long-day conditions. In addition, the haf1 hd1 double mutant headed as late as hd1 plants under short-day conditions but exhibited a heading date similar to haf1 under long-day conditions, thus indicating that HAF1 may determine heading date mainly through Hd1 under short-day conditions. Moreover, high levels of Hd1 accumulate in haf1. Our results suggest that HAF1 is essential to precise modulation of the timing of Hd1 accumulation during the photoperiod response in rice.
Process-oriented disaster response management: a structured literature review
Authors: Hofmann Marlen , Betke Hans , Sackmann Stefan 
Published: August 2015

Purpose– The application of business process methods in the domain of disaster response management (DRM) is seen as promising approach due to the similarity of business processes and disaster response processes at the general structure and goals. But up to now only a few approaches were able to handle the special characteristics of the DRM domain. Thus, the purpose of this paper is to identify the existing approaches and analyze them for the discussion of general requirements for applying methods and tools from business process management to DRM.Design/methodology/approach– A structured literature review covering a wide field of information system-related publications (conferences and journals) is used to identify and classify general requirements discussed as the state of the art.Findings– The work in this paper resulted in a suitable classification of requirements for the development of process-oriented DRM approaches deduced from the existing work. This was used to outline and analyze the current research landscape of this topic and identify research gaps as well as existing limitations.Research limitations/implications– Although the review of the state of the art is based on a wide set of publication databases, there may exist relevant research papers which have not been taken into consideration.Originality/value– The elaborated requirements provide value for both the research community and practitioners. They can be considered to develop new or improve existing DRM systems and, thus, to exploit the potentials of process-oriented IT in supporting DRM in the case of disaster.
The relationships among JIT, TQM and production operations performance
Authors: Chen Zhixiang 
Published: August 2015

Purpose– The purpose of this paper is to investigate the relationships among just-in-time (JIT), total quality management (TQM) and production operations performance (POP) based on the survey data from Chinese manufacturing firms, provide managerial implications of effectively implementing JIT and TQM for firms in developing countries.Design/methodology/approach– Questionnaires were collected from 173 Chinese manufacturing firms. Three hypotheses on the relationships among JIT, TQM and POP were first established based on literature review. Empirical study method of structural equation modeling using SPSS and AMOS as data processing tools is employed to test the hypotheses.Findings– The results show that, on one hand, JIT positively influences TQM and POP; on the other hand, although TQM also has positive relationship with POP, the relationship is not significant at a given significant level. Meanwhile, the author survey also shows that JIT and TQM have complementary relationship, i.e., JIT and TQM can benefit each other in a firm.Practical implications– TQM is a base of implementing JIT, it is valuable to concurrently implement JIT and TQM in manufacturing firms in order to improve the POP.Originality/value– It is believed that this research reveals new insights about the JIT and TQM implementation in Chinese manufacturing firms; it has practice value for firms to improve the implementation performance of JIT and TQM.
“You would expect the successful person to be the man”
Authors: Jones Sally 
Published: August 2015

Purpose– This paper aims to to explore power and legitimacy in the entrepreneurship education classroom by using Pierre Bourdieu’s sociological and educational theories. It highlights the pedagogic authority invested in educators and how this may be influenced by their assumptions about the nature of entrepreneurship. It questions the role of educators as disinterested experts, exploring how power and gendered legitimacy “play out” in staff–student relationships and female students’ responses to this.Design/methodology/approach– A multiple-method, qualitative case study approach is taken, concentrating on a depth of focus in one UK’s higher education institution (HEI) and on the experiences, attitudes and classroom practices of staff and students in that institution. The interviews, with an educator and two students, represent a self-contained story within the more complex story of the case study.Findings– The interviewees’ conceptualization of entrepreneurship is underpinned by acceptance of gendered norms, and both students and staff misrecognize the masculinization of entrepreneurship discourses that they encounter as natural and unquestionable. This increases our understanding of symbolic violence as a theoretical construct that can have real-world consequences.Originality/value– The paper makes a number of theoretical and empirical contributions. It addresses an important gap in the literature, as educators and the impact of their attitudes and perceptions on teaching and learning are rarely subjects of inquiry. It also addresses gaps and silences in understandings of the gendered implications of HE entrepreneurship education more generally and how students respond to the institutional arbitration of wider cultural norms surrounding entrepreneurship. In doing so, it challenges assertions that Bourdieu’s theories are too abstract to have any empirical value, by bridging the gap between symbolic violence as a theory and its manifestation in teaching and learning practices.
The potential for fungal biopesticides to reduce malaria transmission under diverse environmental conditions
Authors: Heinig Rebecca L. , Paaijmans Krijn P. , Hancock Penelope A. , Thomas Matthew B. 
Published: August 2015

Summary

The effectiveness of conventional malaria vector control is being threatened by the spread of insecticide resistance. One promising alternative to chemicals is the use of naturally occurring insect‐killing fungi. Numerous laboratory studies have shown that isolates of fungal pathogens such as Beauveria bassiana can infect and kill adult mosquitoes, including those resistant to chemical insecticides.

Unlike chemical insecticides, fungi may take up to a week or more to kill mosquitoes following exposure. This slow kill speed can still reduce malaria transmission because the malaria parasite itself takes at least eight days to complete its development within the mosquito. However, both fungal virulence and parasite development rate are strongly temperature‐dependent, so it is possible that biopesticide efficacy could vary across different transmission environments.

We examined the virulence of a candidate fungal isolate against two key malaria vectors at temperatures from 10 to 34 °C. Regardless of temperature, the fungus killed more than 90% of exposed mosquitoes within the predicted duration of the malarial extrinsic incubation period, a result that was robust to realistic diurnal temperature variation.

We then incorporated temperature sensitivities of a suite of mosquito, parasite and fungus life‐history traits that are important determinants of malaria transmission into a stage‐structured malaria transmission model. The model predicted that, at achievable daily fungal infection rates, fungal biopesticides have the potential to deliver substantial reductions in the density of malaria‐infectious mosquitoes across all temperatures representative of malaria transmission environments.

Synthesis and applications. Our study combines empirical data and theoretical modelling to prospectively evaluate the potential of fungal biopesticides to control adult malaria vectors. Our results suggest that Beauveria bassiana could be a potent tool for malaria control and support further development of fungal biopesticides to manage infectious disease vectors.
Butterfly abundance is determined by food availability and is mediated by species traits
Authors: Curtis Robin J. , Brereton Tom M. , Dennis Roger L. H. , Carbone Chris , Isaac Nick J. B. 
Published: August 2015

SummaryUnderstanding the drivers of population abundance across species and sites is crucial for effective conservation management. At present, we lack a framework for predicting which sites are likely to support abundant butterfly communities.We address this problem by exploring the determinants of abundance among 1111 populations of butterflies in theUK, spanning 27 species on 54 sites. Our general hypothesis is that the availability of food resources is a strong predictor of population abundance both within and between species, but that the relationship varies systematically with species’ traits.We found strong positive correlations between butterfly abundance and the availability of food resources. Our indices of host plant and nectar are both significant predictors of butterfly population density, but the relationship is strongest for host plants, which explain up to 36% of the inter‐site variance in abundance for some species.Among species, the host plant–abundance relationship is mediated by butterfly species traits. It is strongest among those species with narrow diet breadths, low mobility and habitat specialists. Abundance for species with generalist diet and habitat associations is uncorrelated with our host plant index.The host plant–abundance relationship is more pronounced on sites with predominantly north‐facing slopes, suggesting a role for microclimate in mediating resource availability.Synthesis and applications. We have shown that simple measures can be used to help understand patterns in abundance at large spatial scales. For some butterfly species, population carrying capacity on occupied sites is predictable from information about the vegetation composition. These results suggest that targeted management to increase host plant availability will translate into higher carrying capacity. AmongUKbutterflies, the species that would benefit most from such intervention have recently experienced steep declines in both abundance and distribution. The host plant–abundance relationship we have identified is likely to be transferrable to other systems characterized by strong interspecific interactions across trophic levels. This raises the possibility that the quality of habitat patches for specialist species is estimable from rapid assessment of the host plant resource.
A Novel Method for Removing a Spinal Cord with Attached Cervical Ganglia from a Pediatric Decedent
Authors: Peterson Jo Elle G. , Love Jennifer C. , Pinto Deborrah C. , Wolf Dwayne A. , Sandberg Glenn 
Published: August 2015

AbstractA diagnosis of child abuse is dependent on a comprehensive and accurate assessment of injury in the context of a thorough investigation. However, signatures of trauma are often subtle and interpretation can be very difficult. Recently, researchers have refocused their attention from the head to the neck in search of traumatic signatures of abusive head trauma. HCIFS has developed a technique to remove the cervical spinal cord with the ganglia attached that is less destructive and more time and cost efficient than alternative methods previously published. Once removed, the dorsal nerve roots and ganglia are evaluated for the presence of hemorrhage. The authors performed a small pilot study using the novel method to evaluate 20 decedents with a history of blunt force trauma and eight without a traumatic history. Fifteen of the traumatic deaths and two of the nontraumatic deaths were found to have dorsal nerve root and/or ganglia hemorrhage.
Effect of flavonoids on remineralization of artificial root caries
Authors: Epasinghe DJ , Yiu CKY , Burrow MF 
Published: August 2015

AbstractBackgroundThis study compared the effects of three flavonoids, including proanthocyanidin, naringin and quercetin on remineralization of artificial root caries.MethodsDemineralized root fragments (n = 75) were randomly divided into five groups for treatment with the remineralizing agents for 10 minutes: (1) 6.5% proanthocyanidin; (2) 6.5% naringin; (3) 6.5% quercetin; (4) 1000 ppm fluoride; and (5) deionized water (control). The demineralized samples were pH‐cycled through treatment solutions, acidic buffer and neutral buffer for eight days at six cycles per day. The remineralization effects were evaluated using Knoop microhardness, transverse microradiography (lesion depth and mineral loss) and confocal laser scanning microscopy. Microhardness at different lesion depths was analysed with two‐way ANOVA and Tukey's test, while lesion depths and mineral loss were analysed with one‐way ANOVA and Tukey's test.ResultsArtificial caries lesions treated with fluoride and flavonoids showed significantly greater hardness than the control group (p < 0.05). Both lesion depths and mineral loss of the flavonoid treated groups were significantly lower than the control group (p < 0.05), but significantly higher than the fluoride treated group. No significant difference in lesion depth and mineral loss was found among the three flavonoids (p > 0.05).ConclusionsAll three flavonoids showed positive effects on artificial root caries remineralization, which are significantly lower than that of 1000 ppm fluoride.
Oral health of schoolchildren in Western Australia
Authors: Arrow P 
Published: August 2015

AbstractBackgroundThe West Australian School Dental Service (SDS) provides free, statewide, primary dental care to schoolchildren aged 5–17 years. This study reports on an evaluation of the oral health of children examined during the 2014 calendar year.MethodsChildren were sampled, based on their date of birth, and SDS clinicians collected the clinical information. Weighted mean values of caries experience were presented. Negative binomial regression modelling was undertaken to test for factors of significance in the rate of caries occurrence.ResultsData from children aged 5–15 years were used (girls = 4616, boys = 4900). Mean dmft (5–10‐year‐olds), 1.42 SE 0.03; mean DMFT (6–15‐year‐olds), 0.51 SE 0.01. Negative binomial regression model of permanent tooth caries found higher rates of caries in children who were from non‐fluoridated areas (RR 2.1); Aboriginal (RR 2.4); had gingival inflammation (RR 1.5); lower ICSEA level (RR 1.4); and recalled at more than 24‐month interval (RR 1.8).ConclusionsThe study highlighted poor dental health associated with living in non‐fluoridated areas, Aboriginal identity, poor oral hygiene, lower socioeconomic level and having extended intervals between dental checkups. Timely assessments and preventive measures targeted at groups, including extending community water fluoridation, may assist in further improving the oral health of children in Western Australia.
Job satisfaction among ‘migrant dentists’ in Australia: implications for dentist migration and workforce policy
Authors: Balasubramanian M , Spencer AJ , Short SD , Watkins K , Chrisopoulos S , Brennan DS 
Published: August 2015

AbstractBackgroundMigrants occupy a significant proportion of the dental workforce in Australia. The objectives of this study were to assess the level of job satisfaction of employed migrant dentists in Australia, and to examine the association between various migrant dentist characteristics and job satisfaction.MethodsAll migrant dentists resident in Australia were surveyed using a five‐point Likert scale that measured specific aspects of job, career and satisfaction with area and type of practice.ResultsA total of 1022 migrant dentists responded to this study; 974 (95.4%) were employed. Responses for all scales were skewed towards strongly agree (scores ≥4). The overall scale varied by age group, marital status, years since arrival to Australia and specialist qualification (chi‐square, p < 0.05). In a multivariate logistic regression model, there was a trend towards greater satisfaction amongst older age groups. Dentists who migrated through the examination pathway (mainly from low‐ and middle‐income countries) had a lower probability of being satisfied with the area and type of practice (OR = 0.71; 0.51–0.98), compared with direct‐entry migrant dentists (from high‐income countries).ConclusionsThe high level of job satisfaction of migrant dentists reflects well on their work‐related experiences in Australia. The study offers policy suggestions towards support for younger dentists and examination pathway migrants, so they have appropriate skills and standards to fit the Australian health care environment.