bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deficiency of Endogenous Acute-Phase Serum Amyloid A Protects apoE
−/−
Mice From Angiotensin II–Induced Abdominal Aortic Aneurysm Formation
Authors: Webb Nancy R. , De Beer Maria C. , Wroblewski Joanne M. , Ji Ailing , Bailey William , Shridas Preetha , Charnigo Richard J. , Noffsinger Victoria P. , Witta Jassir , Howatt Deborah A. , Balakrishnan Anju , Rateri Debra L. , Daugherty Alan , De Beer Frederick C. 
Published: March 2015

Objective—
Rupture of abdominal aortic aneurysm (AAA), a major cause of death in the aged population, is characterized by vascular inflammation and matrix degradation. Serum amyloid A (SAA), an acute-phase reactant linked to inflammation and matrix metalloproteinase induction, correlates with aortic dimensions before aneurysm formation in humans. We investigated whether SAA deficiency in mice affects AAA formation during angiotensin II (Ang II) infusion.

Approach and Results—

Plasma SAA increased ≈60-fold in apoE
−/−
mice 24 hours after intraperitoneal Ang II injection (100 μg/kg; n=4) and ≈15-fold after chronic 28-day Ang II infusion (1000 ng/kg per minute; n=9). AAA incidence and severity after 28-day Ang II infusion was significantly reduced in apoE
−/−
mice lacking both acute-phase SAA isoforms (SAAKO; n=20) compared with apoE
−/−
mice (SAAWT; n=20) as assessed by in vivo ultrasound and ex vivo morphometric analyses, despite a significant increase in systolic blood pressure in SAAKO mice compared with SAAWT mice after Ang II infusion. Atherosclerotic lesion area of the aortic arch was similar in SAAKO and SAAWT mice after 28-day Ang II infusion. Immunostaining detected SAA in AAA tissues of Ang II–infused SAAWT mice that colocalized with macrophages, elastin breaks, and enhanced matrix metalloproteinase activity. Matrix metalloproteinase-2 activity was significantly lower in aortas of SAAKO mice compared with SAAWT mice after 10-day Ang II infusion.

Conclusions—
Lack of endogenous acute-phase SAA protects against experimental AAA through a mechanism that may involve reduced matrix metalloproteinase-2 activity.
Nonalcoholic Fatty Liver Disease and Vascular Function
Authors: Long Michelle T. , Wang Na , Larson Martin G. , Mitchell Gary F. , Palmisano Joseph , Vasan Ramachandran S. , Hoffmann Udo , Speliotes Elizabeth K. , Vita Joseph A. , Benjamin Emelia J. , Fox Caroline S. , Hamburg Naomi M. 
Published: March 2015

Objective—
Patients with nonalcoholic fatty liver disease (NAFLD) have an increased risk of cardiovascular disease; however, it is not known whether NAFLD contributes to cardiovascular disease independent of established risk factors. We examined the association between NAFLD and vascular function.

Approach and Results—

We conducted a cross-sectional study of 2284 Framingham Heart Study participants without overt cardiovascular disease who had liver fat attenuation measured on computed tomography and who had measurements of vascular function and covariates. We evaluated the association between NAFLD and vascular function using multivariable partial correlations adjusting for age, sex, cohort, smoking, diabetes mellitus, hyperlipidemia, hypertension, body mass index, and visceral adipose tissue. The prevalence of NAFLD in our sample (mean age, 52±12 years; 51.4% women) was 15.3%. In age-, sex-, and cohort-adjusted analyses, greater liver fat was modestly associated with lower flow-mediated dilation (
r
=−0.05;
P
=0.02), lower peripheral arterial tonometry ratio (
r
=−0.20;
P
<0.0001), higher carotid-femoral pulse wave velocity (
r
=0.13;
P
<0.0001), and higher mean arterial pressure (
r
=0.11;
P
<0.0001). In multivariable-adjusted models, NAFLD remained associated with higher mean arterial pressure (
r
=0.06;
P
=0.005) and lower peripheral arterial tonometry ratio (
r
=−0.12;
P
<0.0001). The association between NAFLD and peripheral arterial tonometry ratio persisted after further adjustment for body mass index and visceral adipose tissue.

Conclusions—
For multiple measures of vascular function, the relationship with NAFLD appeared largely determined by shared cardiometabolic risk factors. The persistent relationship with reduced peripheral arterial tonometry response beyond established risk factors suggests that NAFLD may contribute to microvascular dysfunction.
Daily variations in vertical distribution of crustacean zooplankton in a mountain lake (Lake Tinquilco, 39°S, Araucania Region) in Chile
Authors: De los Ríos-Escalante Patricio , Hauenstein Enrique , Acevedo Patricio 
Published: March 2015

The community of crustacean zooplankton in lakes can show temporal vertical variations in composition and abundance due to the influence of exposure to natural ultraviolet radiation. The aim of the present study was to analyse the zooplankton composition both at the surface and in the water column, and at different times during the day and night, in a northern Patagonian mountain lake (Lake Tinquilco, 39°S, 763 m a.s.l.) in Chile. Eight samples were collected in two days: four samples between the surface and 5 m depth, and four between the surface and 20 m depth. Based on these data, the corresponding values of the Shannon diversity index were estimated. In samples collected at 0-5 m depth, low species numbers were found in comparison with samples collected at 0-20 m. At 0-5 m, the highest biodiversity was observed in the evening, whereas at 0-20 m depth the highest biodiversity was observed in the morning and early afternoon. As a possible cause for this variation, the exposure to natural ultraviolet radiation would be the most probable, since that harmful radiation can penetrate into the superficial part of the water column.
Effects of hyperbaric oxygen therapy on rat facial skin
Authors: Sula B , Ekinci C , Uçak H , Uçmak D , Akkurt ZM , Aktaş A , Arica M , Deveci E 
Published: March 2015

Introduction: We used immunohistochemistry to investigate the histopathologic effects of hyperbaric oxygen (HBO) on the facial skin of rats. Material and methods: Rats in the HBO group ( n = 6) were placed in a 20 L HBO chamber at 2.5 atmospheres absolute at 25–26°C with 100% oxygen for 90 min/day for 7 days. Following euthanasia, sections of facial skin were removed for examination. Results: Epidermal hyperplasia and degeneration, basal-cell hypertrophy, subepithelial fibrosis, and increased connective tissue were observed in the HBO group. E-cadherin expression was reduced in the epidermis, hair follicles, and sebaceous glands in HBO-treated rats relative to control animals. HBO treatment was associated with vimentin immunoreactivity in fibroblasts, endothelial cells, and the bulbus pilorum of a subset of hair follicles. It also resulted in increased type IV collagen expression within the connective tissue in the hair follicles and sebaceous glands. Conclusion: The HBO group demonstrated epidermal hyperplasia and degeneration, basal-cell hypertrophy, and subepithelial fibrosis. In addition, HBO decreased E-cadherin expression, which suggests that HBO may impair intracellular adhesion. Expression of vimentin and type IV collagen was also observed in the dermis. Increased connective tissue, hemorrhage, and mononuclear cell infiltration were observed in the dermis of HBO-treated animals. Thus, HBO has effects on the structures of the epidermis and dermis.
A high-performance complementary inverter based on transition metal dichalcogenide field-effect transistors
Authors: Cho Ah-Jin , Park Kee Chan , Kwon Jang-Yeon 
Published: March 2015

Abstract
For several years, graphene has been the focus of much attention due to its peculiar characteristics, and it is now considered to be a representative 2-dimensional (2D) material. Even though many research groups have studied on the graphene, its intrinsic nature of a zero band-gap, limits its use in practical applications, particularly in logic circuits. Recently, transition metal dichalcogenides (TMDs), which are another type of 2D material, have drawn attention due to the advantage of having a sizable band-gap and a high mobility. Here, we report on the design of a complementary inverter, one of the most basic logic elements, which is based on a MoS2 n-type transistor and a WSe2 p-type transistor. The advantages provided by the complementary metal-oxide-semiconductor (CMOS) configuration and the high-performance TMD channels allow us to fabricate a TMD complementary inverter that has a high-gain of 13.7. This work demonstrates the operation of the MoS2 n-FET and WSe2 p-FET on the same substrate, and the electrical performance of the CMOS inverter, which is based on a different driving current, is also measured.
RETRACTED ARTICLE: Effect of apoptosis in neural stem cells treated with sevoflurane
Authors: Qiu Jianlei , Shi Pengcai , Mao Wude , Zhao Yuyi , Liu Wenshuai , Wang Yuelan 
Published: March 2015

Abstract
Background
At present, sevoflurane inhalation anesthesia used on infants is well-known. But long-time exposure to inhalation anesthetic could cause neurologic disorder, especially nerve degeneration in infant and developing brain. The central nervous system degeneration of infants could affect the memory and cognitive function. γ-Aminobutyric acid (GABA) is a known inhibitory neurotransmitter in central nervous system. Inhalation anesthetic sevoflurane may activate GABAA receptor to inhibit central nervous system, leading to apoptosis of neural degeneration, cognitive dysfunction in the critical period of brain development.

Methods
Neural stem cells were derived from Wistar embryos, cultured in vitro. Third generation of neural stem cells were randomly divided into four groups according to cultured suspension: Sevoflurane group (Group S), GABAA receptor antagonists, Bicuculline group (Group B), Sevoflurane + GABAA receptor antagonists, Bicuculline group (Group S + B), dimethyl sulphoxide (DMSO) group (Group D). Group B and Group D did not receive sevoflurane preconditioning. Group S and Group S + B were pretreated with 1 minimum alveolar concentration (MAC) sevoflurane for 0 h, 3 h, 6 h, and 12 h. Group S + B and Group B were pretreated with bicuculline (10 uM). Group D was treated with DMSO (10 uL/mL). After treatments above, all groups were cultured for 48 h. Then we measured the cells viability by Cell Counting Kit (CCK-8) assay, cytotoxicity by Lactate Dehydrogenase (LDH) assay, apoptosis ratio with Annexin V/propidium iodide (PI) staining by flow cytometry, and the expression of GABAAR, anti-apoptotic protein Bcl-2, pro-apoptotic protein Bax and Caspase-3 by western blotting.

Results
After exposing to sevoflurane for 0 h, 3 h, 6 h, and 12 h with 1MAC, we found that cell viability obviously decreased and cytotoxicity increased in time-dependent way. And Annexin V/PI staining indicated increased apoptosis ratio by flow cytometry. The protein level of GABAA receptor, pro-apoptotic protein Bax and apoptosis protein Caspase-3 increased; while anti-apoptotic protein Bcl-2 decreased. And bicuculline could reverse all detrimental results caused by sevoflurane.

Conclusion
Sevoflurane can inhibit the central nervous system by activating GABAA, resulting in apoptosis of neural stem cells, thus leading to the NSCs degeneration.
Histone deacetylase inhibitor, suberoylanilide hydroxamic acid (SAHA), enhances anti-tumor effects of the poly (ADP-ribose) polymerase (PARP) inhibitor olaparib in triple-negative breast cancer cells
Authors: Min Ahrum , Im Seock-Ah , Kim Debora Keunyoung , Song Sang-Hyun , Kim Hee-Jun , Lee Kyung-Hun , Kim Tae-Yong , Han Sae-Won , Oh Do-Youn , Kim Tae-You , O’Connor Mark J , Bang Yung-Jue 
Published: March 2015

Abstract
Introduction
Olaparib, a poly (ADP-ribose) polymerase (PARP) inhibitor, has been found to have therapeutic potential for treating cancers associated with impaired DNA repair capabilities, particularly those with deficiencies in the homologous recombination repair (HRR) pathway. Histone deacetylases (HDACs) are important for enabling functional HRR of DNA by regulating the expression of HRR-related genes and promoting the accurate assembly of HRR-directed sub-nuclear foci. Thus, HDAC inhibitors have recently emerged as a therapeutic agent for treating cancer by inhibiting DNA repair. Based on this, HDAC inhibition could be predicted to enhance the anti-tumor effect of PARP inhibitors in cancer cells by blocking the HRR pathway.

Methods
We determined whether suberoylanilide hydroxamic acid (SAHA), a HDAC inhibitor, could enhance the anti-tumor effects of olaparib on breast cancer cell lines using a cytotoxic assay, cell cycle analysis, and Western blotting. We evaluated how exposure to SAHA affects the expression of HRR-associated genes. The accumulation of DNA double strand breaks (DSBs) induced by combination treatment was assessed. Induction of autophagy was monitored by imaging green fluorescent protein-tagged microtubule-associated protein 1A/1B-light chain 3 (LC3) expression following co-treatment with olaparib and SAHA. These in vitro data were validated in vivo using a human breast cancer xenograft model.

Results
Triple-negative breast cancer cell (TNBC) lines showed heterogeneous responses to the PARP and HDAC inhibitors. Co-administration of olaparib and SAHA synergistically inhibited the growth of TNBC cells that expressed functional Phosphatase and tensin homolog (PTEN). This effect was associated with down-regulation of the proliferative signaling pathway, increased apoptotic and autophagic cell death, and accumulation of DNA damage. The combined anti-tumor effect of olaparib and SAHA was also observed in a xenograft model. These data suggest that PTEN expression in TNBC cells can sensitize the cell response to simultaneous inhibition of PARP and HDAC both in vitro and in vivo.

Conclusion
Our findings suggest that expression of functional PTEN may serve as a biomarker for selecting TNBC patients that would favorably respond to a combination of olaparib with SAHA. This provides a strong rationale for treating TNBC patients with PTEN expression with a combination therapy consisting of olaparib and SAHA.
Coalescence-avoiding joint probabilistic data association based on bias removal
Authors: Jing Peiliang , Xu Shiyou , Li Xian , Chen Zengping 
Published: March 2015

AbstractIn order to deal with the track coalescence problem of the joint probabilistic data association (JPDA) algorithm, a novel approach from a state bias removal point of view is developed in this paper. The factors that JPDA causes the state bias are analyzed, and the direct computation equation of the bias in the ideal case is given. Then based on the definitions of target detection hypothesis and target-to-target association hypothesis, the bias estimation is extended to the general and practical case. Finally, the estimated bias is removed from the state updated by JPDA to generate the unbiased state. The results of Monte Carlo simulations show that the proposed method can handle track coalescence and presents better performance when compared with the traditional methods.
Dynamic switching off algorithms for pico base stations in heterogeneous cellular networks
Authors: Wu Jie , Jin Shi , Jiang Lei , Wang Gang 
Published: March 2015

Abstract
The densification of pico base stations (PBSs) in heterogenous cellular networks (Hetnets) causes redundant energy consumption of the whole network during low traffic periods. In order to address this problem, we take advantage of the traffic load fluctuation over time and area to adapt the necessary resource to the actual traffic demand by introducing sleep mode to the PBSs. To achieve this target, we propose two centralized algorithms (i.e., the heuristic algorithm and the progressive algorithm) in this paper to dynamically switch off the unnecessary PBSs, both of which can track the traffic variation well. We design a utility function of some key factors considered particularly for PBSs in Hetnets. These algorithms rely on the utility function to switch off the redundant PBSs, enabling the working mode of all PBSs be reconfigured periodically. The progressive algorithm is proposed to overcome the inefficiency of the heuristic algorithm. The simulations demonstrate that the execution time of the progressive algorithm is at most one third of that of the heuristic algorithm, which enables the network to respond to the traffic variation more promptly. Besides, the progressive algorithm can switch off more PBSs than the heuristic algorithm while slightly affects the network blocking probability, which indicates that the progressive algorithm has a better potential to save energy. Moreover, simulations also reveal that some key parameters all have nonnegligible influence on the performance of our algorithms. These parameters should be tuned well to trade spare network resource for energy saving.
Using molecular dynamics to quantify the electrical double layer and examine the potential for its direct observation in the in-situ TEM
Authors: Welch David A , Mehdi B Layla , Hatchell Hannah J , Faller Roland , Evans James E , Browning Nigel D 
Published: March 2015

AbstractUnderstanding the fundamental processes taking place at the electrode-electrolyte interface in batteries will play a key role in the development of next generation energy storage technologies. One of the most fundamental aspects of the electrode-electrolyte interface is the electrical double layer (EDL). Given the recent development of high spatial resolution in-situ electrochemical fluid cells for scanning transmission electron microscopy (STEM), there now exists the possibility that we can directly observe the formation and dynamics of the EDL. In this paper we predict electrolyte structure within the EDL using classical models and atomistic Molecular Dynamics (MD) simulations. Classical models are found to greatly differ from MD in predicted concentration profiles. It is thus suggested that MD must be used in order to accurately predict STEM images of the electrode-electrolyte interface. Using MD and image simulation together for a high contrast electrolyte (the high atomic number CsCl electrolyte), it is determined that, for a smooth interface, concentration profiles within the EDL should be visible experimentally. When normal experimental parameters such as rough interfaces and low-Z electrolytes (like those used in Li-ion batteries) are considered, observation of the EDL appears to be more difficult.
Neutrophil elastase promotes myofibroblast differentiation in lung fibrosis
Authors: Gregory Alyssa D , Kliment Corrine R , Metz Heather E , Kim Kyoung-Hee , Kargl Julia , Agostini Brittani A , Crum Lauren T , Oczypok Elizabeth A , Oury Tim A , Houghton A McGarry 
Published: March 2015

Abstract
IPF is a progressive lung disorder characterized by fibroblast proliferation and myofibroblast differentiation. Although neutrophil accumulation within IPF lungs has been negatively correlated with outcomes, the role played by neutrophils in lung fibrosis remains poorly understood. We have demonstrated previously that NE promotes lung cancer cell proliferation and hypothesized that it may have a similar effect on fibroblasts. In the current study, we show that NE−/− mice are protected from asbestos-induced lung fibrosis. NE−/− mice displayed reduced fibroblast and myofibroblast content when compared with controls. NE directly both lung fibroblast proliferation and myofibroblast differentiation in vitro, as evidenced by proliferation assays, collagen gel contractility assays, and αSMA induction. Furthermore, αSMA induction occurs in a TGF-β-independent fashion. Treatment of asbestos-recipient mice with ONO-5046, a synthetic NE antagonist, reduced hydroxyproline content. Thus, the current study points to a key role for neutrophils and NE in the progression of lung fibrosis. Lastly, the study lends rationale to use of NE-inhibitory approaches as a novel therapeutic strategy for patients with lung fibrosis.
Modern low-cost internet technologies for promotion of educational services of universities
Authors: Губина Олеся , Gubina Olesya 
Published: March 2015

The rapid development of the Internet-sphere involves an active expansion of the range of different online resources, technologies and opportunities for organizations for marketing purposes. This article is devoted to the promotion of educational services of universities using the Internet. In this paper we study the possibility of promotion of educational services of universities using Internet technologies that do not require significant financial investments, we also present the analysis of existing modern internet sites, which are used by universities for marketing purposes. The author explores the modern educational portals, sites of educational institutions, specialized sites reviews, social networks, search engines, portals and various themed e-mail services with respect to the efficiency of promotion of university educational services, identifies ways to promote university educational services via the Internet, developing recommendations to enhance their effectiveness. The paper presents the characteristics of the main groups of Internet resources, through which the promotion of educational services of universities can be made possible, gives examples of types of posting information about universities in order to promote educational services. The author gives her own research based on practical experience and work on online promotion of educational services of universities.
According to the analysis of literature and the author´s studies, which reflect the main trends in the development of Internet technologies of promotion of educational services of universities, compiled a table summarizing the effectiveness of online resources and recommended content and low-cost methods of promotion of educational services of universities.
Current problems of practice-oriented education and training for workers in Russia
Authors: Романова Маргарита , Romanova Margarita , Чурилова Ирина , Churilova Irina 
Published: March 2015

The article deals with the problems of practice-oriented education in preparing workforce in Russia. Decline in prestige and deficit of the supply of workers remain one of the most pressing problems of the state and society, which has negative impacts on the economy, the social sphere, the general state of national culture.
According to the authors, to fill this lack requires, implementing an independent state policy in the field of education and science, aimed at active three-way interaction of educational institutions training mid-level professionals, students and employers. Such interaction can be achieved under the condition of students, representatives of educational institutions and employers&#180; awareness of high responsibility for the present and the future of the state, mutual, purposeful and active participation of everyone. The result of this work should include the training of highly educated and cultural practitioners, demanded on the labor market specialists.
As an example of an effective solution of the identified problem, the authors consider the operations of the multipurpose center for appliedqualifications at Russian State University of Tourism and Service (Moscow), which is a new model of the system of staff training and the formation of applied qualifications.
The essence of this model is centered on a more active involvement of employers in the activities of educational institutions: in making strategic and operational decisions in the field of vocational training, in funding training programs, in the formation of standards of vocational training of students, teacher training, training and internships on industrial base, in paying costs of training which is provided by the company.
Multifunction centers for applied qualifications, according to the authors, will allow the state to overcome youth unemployment, society to enhance the contribution ofvocational training in the economic development of Russia, students to find jobs, and employers to overcome the deficiency of worker qualifications, distrust towards the system of vocational education and create incentives to partnership.