bluuebunny/crossref_metadata_2025_split huggingface dataset:
American Trypanosomiasis Infection in Fattening Pigs from the South‐East of Mexico
Authors: Jiménez‐Coello M. , Acosta‐Viana K. Y. , Guzman‐Marin E. , Ortega‐Pacheco A. 
Published: September 2012

SummaryAmerican Trypanosomiasis (AT) is an infectious parasitic disease produced by the protozoa Trypanosoma cruzi (T. cruzi). Infection is acquired by vectorial via but can also be transmitted congenitally, by ingestion of an infected host, by transfusion with contaminated blood or transplant of organs from an infected donor. Currently, AT is widely distributed from the South of the United States to South America. In Mexico, the presence of the parasite has been reported throughout the country where several reservoirs such as dogs, opossums, rats and cats have been identified. Yucatan is in the south‐east of Mexico where AT is endemic and has been reported since 1940s. There is little information about the role of pigs as reservoirs of T. cruzi. The frequency of specific antibodies against T. cruzi was determined in fattening pigs from Yucatan, Mexico. After sampling in the 3 main areas of pig production in the state, IgG ELISA and Western blot were performed to identify seropositive cases. Association of farm size, farm area and production system with infected pigs was evaluated. From 273 sampled pigs, 5.4% (n = 15) positive cases were found. No association with evaluated factors and infected pigs was found. Pigs are also reservoirs of T. cruzi in the studied area. These findings are considered important to improve vectorial control in the area in order to avoid the parasite infection in animal populations destined for human consumption and avoid further transmission to humans.
Promotion of couples’ voluntary HIV counselling and testing in Lusaka, Zambia by influence network leaders and agents
Authors: Wall Kristin M , Kilembe William , Nizam Azhar , Vwalika Cheswa , Kautzman Michelle , Chomba Elwyn , Tichacek Amanda , Sardar Gurkiran , Casanova Deborah , Henderson Faith , Mulenga Joseph , Kleinbaum David , Allen Susan 
Published: September 2012

ObjectivesHypothesising that couples’ voluntary counselling and testing (CVCT) promotions can increase CVCT uptake, this study identified predictors of successful CVCT promotion in Lusaka, Zambia.DesignCohort study.SettingLusaka, Zambia.Participants68 influential network leaders (INLs) identified 320 agents (INAs) who delivered 29 119 CVCT invitations to heterosexual couples.InterventionThe CVCT promotional model used INLs who identified INAs, who in turn conducted community-based promotion and distribution of CVCT invitations in two neighbourhoods over 18 months, with a mobile unit in one neighbourhood crossing over to the other mid-way through.Primary outcomeThe primary outcome of interest was couple testing (yes/no) after receipt of a CVCT invitation. INA, couple and invitation characteristics predictive of couples’ testing were evaluated accounting for two-level clustering.ResultsINAs delivered invitations resulting in 1727 couples testing (6% success rate). In multivariate analyses, INA characteristics significantly predictive of CVCT uptake included promoting in community-based (adjusted OR (aOR)=1.3; 95% CI 1.0 to 1.8) or health (aOR=1.5; 95% CI 1.2 to 2.0) networks versus private networks; being employed in the sales/service industry (aOR=1.5; 95% CI 1.0 to 2.1) versus unskilled manual labour; owning a home (aOR=0.7; 95% CI 0.6 to 0.9) versus not; and having tested for HIV with a partner (aOR=1.4; 95% CI 1.1 to 1.7) or alone (aOR=1.3; 95% CI 1.0 to 1.6) versus never having tested. Cohabiting couples were more likely to test (aOR=1.4; 95% CI 1.2 to 1.6) than non-cohabiting couples. Context characteristics predictive of CVCT uptake included inviting couples (aOR=1.2; 95% CI 1.0 to 1.4) versus individuals; the woman (aOR=1.6; 95% CI 1.2 to 2.2) or couple (aOR=1.4; 95% CI 1.0 to 1.8) initiating contact versus the INA; the couple being socially acquainted with the INA (aOR=1.6; 95% CI 1.4 to 1.9) versus having just met; home invitation delivery (aOR=1.3; 95% CI 1.1 to 1.5) versus elsewhere; and easy invitation delivery (aOR=1.8; 95% CI 1.4 to 2.2) versus difficult as reported by the INA.ConclusionsThis study demonstrated the ability of influential people to promote CVCT and identified agent, couple and context-level factors associated with CVCT uptake in Lusaka, Zambia. We encourage the development of CVCT promotions in other sub-Saharan African countries to support sustained CVCT dissemination.
Home point-of-care international normalised ratio monitoring sustained by a non-selective educational program in children
Authors: Elie Caroline , Cheurfi Radhia , Grazioli Aurélie , Traore Maladon , Souillard Patrick , Boudjemline Younes , Jourdain Patrick , Bajolle Fanny , Lasne Dominique , Bonnet Damien 
Published: September 2012

SummaryAdverse events related to vitamin K antagonist (VKA) therapy might be reduced by point-of-care international normalised ratio (POC INR) monitoring supported by an education program (EP). Our aim was to evaluate the efficacy of a non-selective VKA paediatric EP (regardless of the social, economic, educational or linguistic levels) by analysing the time spent in the therapeutic range (TTR), VKA adverse events and compliance to treatment, and INR control prescriptions. The EP was modified from the pediatric EP previously described but improved by a specifically devised child-focused game. One hundred four consecutive children (median age 8 years) receiving VKA were included in a standardised E P. Patients were in self-testing, and dose adjustments were made by a single physician for three tolerance ranges according to the underlying disease: [2.5–4], [1.8–3.2], and [1.5–2.5]. The median follow-up was 481 days [70–1,001]. The overall TTR was 81.4% [36–100]. The TTR were 74%, 85.6% and 89% for the ranges [2.5–4], [1.8–3.2], and [1.5–2.5], respectively. These results were sustainable during the study period. Only one serious VKA adverse event was recorded. The median number of POC INR tests was 2.5 [1.6–5.7] INR per patient and month. Patients/families performed POC INR when requested in 86.9% of the cases. More than 90% of the families found the EP supportive and wished to follow a long-term reinforcement program. In conclusion, this non-selective child-focused EP for VKA therapy, strongly supported by our dedicated game, is useful in maintaining efficacy, safety and compliance to anticoagulation and its monitoring.
Cardiomyogenesis in the Aging and Failing Human Heart
Authors: Kajstura Jan , Rota Marcello , Cappetta Donato , Ogórek Barbara , Arranto Christian , Bai Yingnan , Ferreira-Martins João , Signore Sergio , Sanada Fumihiro , Matsuda Alex , Kostyla James , Caballero Maria-Virginia , Fiorini Claudia , D'Alessandro David A. , Michler Robert E. , del Monte Federica , Hosoda Toru , Perrella Mark A. , Leri Annarosa , Buchholz Bruce A. , Loscalzo Joseph , Anversa Piero 
Published: September 2012

Background—
Two opposite views of cardiac growth are currently held; one views the heart as a static organ characterized by a large number of cardiomyocytes that are present at birth and live as long as the organism, and the other views the heart a highly plastic organ in which the myocyte compartment is restored several times during the course of life.

Methods and Results—

The average age of cardiomyocytes, vascular endothelial cells (ECs), and fibroblasts and their turnover rates were measured by retrospective
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C birth dating of cells in 19 normal hearts 2 to 78 years of age and in 17 explanted failing hearts 22 to 70 years of age. We report that the human heart is characterized by a significant turnover of ventricular myocytes, ECs, and fibroblasts, physiologically and pathologically. Myocyte, EC, and fibroblast renewal is very high shortly after birth, decreases during postnatal maturation, remains relatively constant in the adult organ, and increases dramatically with age. From 20 to 78 years of age, the adult human heart entirely replaces its myocyte, EC, and fibroblast compartment ≈8, ≈6, and ≈8 times, respectively. Myocyte, EC, and fibroblast regeneration is further enhanced with chronic heart failure.

Conclusions—
The human heart is a highly dynamic organ that retains a remarkable degree of plasticity throughout life and in the presence of chronic heart failure. However, the ability to regenerate cardiomyocytes, vascular ECs, and fibroblasts cannot prevent the manifestations of myocardial aging or oppose the negative effects of ischemic and idiopathic dilated cardiomyopathy.
Measurement of Convective Entrainment Using Lagrangian Particles
Authors: Yeo Kyongmin , Romps David M. 
Published: September 2012

Abstract
Lagrangian particle tracking is used in a large-eddy simulation to study an individual cumulus congestus. This allows for the direct measurement of the convective entrainment rate and of the residence times of entrained parcels within the cloud. The entrainment rate obtained by Lagrangian direct measurement is found to be higher than that obtained using the recently introduced method of Eulerian direct measurement. This discrepancy is explained by the fast recirculation of air in and out of cloudy updrafts, which Eulerian direct measurement is unable to resolve. By filtering these fast recirculations, the Lagrangian calculation produces a result in very good agreement with the Eulerian calculation.
The Lagrangian method can also quantify some aspects of entrainment that cannot be probed with Eulerian methods. For instance, it is found that more than half of the air that is entrained by the cloud during its lifetime is air that was previously detrained by the cloud. Nevertheless, the cloud is highly diluted by entrained air: for cloudy air above 2 km, its mean height of origin is well above the cloud base. This paints a picture of a cloud that rapidly entrains both environmental air and its own detritus.
Identifying Breastfeeding-Sensitive Conditions by Expert Consensus
Authors: Quinn Celia , Bonuck Karen 
Published: September 2012

Background: Infant feeding-related health outcomes need to be consistently defined prior to inclusion in health services research. Objective: To categorize conditions common in infants under 12 months old by their association with breastfeeding for use as outcome measures in a randomized, controlled trial of breastfeeding promotion. Methods: A modified Delphi consensus method synthesized opinions of 13 physician experts on breastfeeding’s association with ICD-9 infant diagnosis codes derived from literature review and medical center experience. A pilot round and 2 subsequent Delphi rounds were used. For the first round, consensus was achieved when more than 80% of experts agreed on a classification for a particular condition, with a predetermined level of certainty based on a 7-point Likert scale. For the second round, consensus was achieved when a majority of experts agreed on the classification from Round 1. Results: An initial 68 diagnosis codes were identified for evaluation by the expert panel. After a pilot round, the codes were refined and condensed, which resulted in 38 diagnoses for categorization into 1 of 3 categories: (1) breastfeeding protects against the condition; (2) breastfeeding may cause or worsen the condition; and (3) breastfeeding is unrelated to the condition. At the conclusion of the process, consensus was achieved on the classification of 31 conditions, and 7 conditions remained unclassified because of a lack of consensus. Conclusions: This study provides a list of conditions common in infants under 12 months of age classified based on relationship to infant feeding method and validated by expert consensus. These conditions, based on readily available insurance claims data, contribute to the standardization of outcome measures used for health services research related to breastfeeding promotion.
Maternal Postadoption Depression, Unmet Expectations, and Personality Traits
Authors: Foli Karen J. , South Susan C. , Lim Eunjung , Hebdon Megan 
Published: September 2012

Background: There are approximately 2 million adoptive parents in the United States and some struggle with depressive symptoms postplacement. We know little about personality traits that may be associated with depression in adoptive parents. Objectives: This study describes the relationships between personality traits, unmet expectations, and maternal postadoption depression. Design: Adoptive mothers ( N = 136) were surveyed for depressive symptoms using the Center for Epidemiologic Studies–Depression Scale (CES-D) and the Edinburgh Postnatal Depression Scale (EPDS). Analyses included correlations and a regression analysis between depressive symptoms and unmet expectations with the Five-Factor Model personality traits (extraversion, agreeableness, conscientiousness, emotional stability, openness to experience) as measured by the Ten-Item Personality Inventory. Results: Both the CES-D and EPDS were significantly, negatively correlated with all five personality dimensions. Mothers whose expectations of themselves as parents matched pre- and postplacement were more likely to be emotionally stable and extraverted. Approximately 36% of the variance in depressive symptoms was explained by personality traits (emotional stability: p < .0001). Conclusions: The postadoption period is a crucial time of transition for mothers and their children. Maternal emotional stability, depressive symptoms, and unmet expectations may affect this period. Mothers who are lower in emotional stability may be at risk for postadoptive depressive symptoms. In planning and providing innovative care that promotes positive mother–child relationships, nurses should assess adoptive mothers for depressive symptoms, emotional stability, and unmet expectations.
Extraskeletal Osteochondroma of the Medial Arch of the Foot
Authors: Sit Yan Kit , Lui Tun Hing 
Published: September 2012

Extraskeletal osteochondromas are benign, slow growing, and often painless neoplasms. Although they are histologically similar to conventional osteochondromas, extraskeletal osteochondromas are not attached to a parent bone. When a well-defined osseous mass in the soft tissue without any direct continuity with the adjacent bone or joint is encountered, a diagnosis of extraskeletal osteochondroma should be strongly considered. It should be identified to avoid unnecessary aggressive surgical treatment. We present a patient with symptomatic extraskeletal osteochondroma of the medial arch of the foot. It was treated by local excision and no evidence of local recurrence clinically or radiographically after 51 months of follow-up. Levels of Evidence: Therapeutic, Level IV, Case study
Allograft Replacement for Absent Native Tissue
Authors: Chaudhury Salma , Wanivenhaus Florian , Fox Alice J. , Warren Russell F. , Doyle Maureen , Rodeo Scott A. 
Published: September 2012

Context: Structural instability due to poor soft tissue quality often requires augmentation. Allografts are important biological substitutes that are used for the symptomatic patient in the reconstruction of deficient ligaments, tendons, menisci, and osteochondral defects. Interest in the clinical application of allografts has arisen from the demand to obtain stable anatomy with restoration of function and protection against additional injury, particularly for high-demand patients who participate in sports. Traditionally, allografts were employed to reinforce weakened tissue. However, they can also be employed to substitute deficient or functionally absent tissue, particularly in the sports medicine setting. Objective: This article presents a series of 6 cases that utilized allografts to restore functionally deficient anatomic architecture, rather than just simply augmenting the degenerated or damaged native tissue. Detailed discussions are presented of the use of allografts as a successful treatment strategy to replace functionally weakened tissue, often after failed primary repairs.
Decreased mitochondrial apoptotic priming underlies stroma-mediated treatment resistance in chronic lymphocytic leukemia
Authors: Davids Matthew S. , Deng Jing , Wiestner Adrian , Lannutti Brian J. , Wang Lili , Wu Catherine J. , Wilson Wyndham H. , Brown Jennifer R. , Letai Anthony 
Published: September 2012

Abstract
Stroma induces treatment resistance in chronic lymphocytic leukemia (CLL), possibly because of alterations in the BCL-2 family of proteins, which are key regulators of apoptosis. We previously developed BH3 profiling, a functional assay that assesses mitochondrial depolarization in response to BH3-only peptides, to measure “apoptotic priming,” the proximity of a cell to the apoptotic threshold. In the present study, we use BH3 profiling to show that CLL cells from the PB are highly primed. Increased priming is associated with improved clinical response and, unexpectedly, with unmutated IGHV status. Coculturing CLL cells in vitro with stroma decreases priming. Using matched PB, BM, and lymph node compartment samples, we found in vivo that BM-derived CLL cells are the least primed. CLL cells cocultured with stroma were treated with the PI3K δ-isoform inhibitor CAL-101 (GS1101). CAL-101 caused CLL cell de-adhesion, leading to increased CLL cell priming. Stimulation of CLL cells with anti-IgM or CXCL12 caused decreased priming that could be reversed by CAL-101. Our results show that inhibition of stromal interactions leading to displacement of CLL cells into the blood by CAL-101 in vivo may increase CLL cell priming, suggesting a mechanism by which agents inducing lymphocyte redistribution might facilitate improved clinical response when used in combina-tion with other therapies.
Targeting NAD+ salvage pathway induces autophagy in multiple myeloma cells via mTORC1 and extracellular signal-regulated kinase (ERK1/2) inhibition
Authors: Cea Michele , Cagnetta Antonia , Fulciniti Mariateresa , Tai Yu-Tzu , Hideshima Teru , Chauhan Dharminder , Roccaro Aldo , Sacco Antonio , Calimeri Teresa , Cottini Francesca , Jakubikova Jana , Kong Sun-Young , Patrone Franco , Nencioni Alessio , Gobbi Marco , Richardson Paul , Munshi Nikhil , Anderson Kenneth C. 
Published: September 2012

Abstract
Malignant cells have a higher nicotinamide adenine dinucleotide (NAD+) turnover rate than normal cells, making this biosynthetic pathway an attractive target for cancer treatment. Here we investigated the biologic role of a rate-limiting enzyme involved in NAD+ synthesis, Nampt, in multiple myeloma (MM). Nampt-specific chemical inhibitor FK866 triggered cytotoxicity in MM cell lines and patient MM cells, but not normal donor as well as MM patients PBMCs. Importantly, FK866 in a dose-dependent fashion triggered cytotoxicity in MM cells resistant to conventional and novel anti-MM therapies and overcomes the protective effects of cytokines (IL-6, IGF-1) and bone marrow stromal cells. Nampt knockdown by RNAi confirmed its pivotal role in maintenance of both MM cell viability and intracellular NAD+ stores. Interestingly, cytotoxicity of FK866 triggered autophagy, but not apoptosis. A transcriptional-dependent (TFEB) and independent (PI3K/mTORC1) activation of autophagy mediated FK866 MM cytotoxicity. Finally, FK866 demonstrated significant anti-MM activity in a xenograft-murine MM model, associated with down-regulation of ERK1/2 phosphorylation and proteolytic cleavage of LC3 in tumor cells. Our data therefore define a key role of Nampt in MM biology, providing the basis for a novel targeted therapeutic approach.
HDAC6 controls the kinetics of platelet activation
Authors: Sadoul Karin , Wang Jin , Diagouraga Boubou , Vitte Anne-Laure , Buchou Thierry , Rossini Thérèse , Polack Benoît , Xi Xiaodong , Matthias Patrick , Khochbin Saadi 
Published: September 2012

AbstractHDAC6, a major cytoplasmic deacetylase, is shown here to fine-tune the kinetics of platelet activation, a process that must be precisely regulated to ensure hemostasis after blood vessel injury while preventing pathologic thrombus formation. The discoid shape of resting platelets in the circulation is maintained by several highly acetylated microtubules organized in a marginal band. During platelet activation, microtubules undergo major reorganizations, which contribute to the shape change of activating platelets. We show that, during these activation-induced shape changes, a dramatic HDAC6-mediated tubulin deacetylation takes place, followed by microtubule reacetylation in spread platelets. In addition, although HDAC6-controlled tubulin deacetylation is not required for platelet activation, the capacity of HDAC6 to prevent tubulin hyperacetylation influences the speed of platelet spreading. These results are particularly important in view of HDAC6 inhibitors being currently used in clinical trials and represent the first example of cell signaling by lysine acetylation in platelet biology.
PTH expands short-term murine hemopoietic stem cells through T cells
Authors: Li Jau-Yi , Adams Jonathan , Calvi Laura M. , Lane Timothy F. , DiPaolo Richard , Weitzmann M. Neale , Pacifici Roberto 
Published: September 2012

AbstractIntermittent parathyroid hormone (iPTH) treatment expands hemopoietic stem and progenitor cells (HSPCs), but the involved mechanisms and the affected HSPC populations are mostly unknown. Here we show that T cells are required for iPTH to expand short-term HSPCs (ST-HSPCs) and improve blood cell engraftment and host survival after BM transplantation. Silencing of PTH/PTH-related protein receptor (PPR) in T cells abrogates the effects of iPTH, thus demonstrating a requirement for direct PPR signaling in T cells. Mechanistically, iPTH expands ST-HSPCs by activating Wnt signaling in HSPCs and stromal cells (SCs) through T-cell production of the Wnt ligand Wnt10b. Attesting to the relevance of Wnt10b, iPTH fails to expand ST-HSPCs in mice with Wnt10b−/− T cells. Moreover, iPTH fails to promote engraftment and survival after BM transplantation in Wnt10b null mice. In summary, direct PPR signaling in T cells and the resulting production of Wnt10b play a pivotal role in the mechanism by which iPTH expands ST-HSPCs. The data suggest that T cells may provide pharmacologic targets for HSPC expansion.
Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis
Authors: Laitala Anu , Aro Ellinoora , Walkinshaw Gail , Mäki Joni M. , Rossi Maarit , Heikkilä Minna , Savolainen Eeva-Riitta , Arend Michael , Kivirikko Kari I. , Koivunen Peppi , Myllyharju Johanna 
Published: September 2012

AbstractAn endoplasmic reticulum transmembrane prolyl 4-hydroxylase (P4H-TM) is able to hydroxylate the α subunit of the hypoxia-inducible factor (HIF) in vitro and in cultured cells, but nothing is known about its roles in mammalian erythropoiesis. We studied such roles here by administering a HIF-P4H inhibitor, FG-4497, to P4h-tm−/− mice. This caused larger increases in serum Epo concentration and kidney but not liver Hif-1α and Hif-2α protein and Epo mRNA levels than in wild-type mice, while the liver Hepcidin mRNA level was lower in the P4h-tm−/− mice than in the wild-type. Similar, but not identical, differences were also seen between FG-4497–treated Hif-p4h-2 hypomorphic (Hif-p4h-2gt/gt) and Hif-p4h-3−/− mice versus wild-type mice. FG-4497 administration increased hemoglobin and hematocrit values similarly in the P4h-tm−/− and wild-type mice, but caused higher increases in both values in the Hif-p4h-2gt/gt mice and in hematocrit value in the Hif-p4h-3−/− mice than in the wild-type. Hif-p4h-2gt/gt/P4h-tm−/− double gene-modified mice nevertheless had increased hemoglobin and hematocrit values without any FG-4497 administration, although no such abnormalities were seen in the Hif-p4h-2gt/gt or P4h-tm−/− mice. Our data thus indicate that P4H-TM plays a role in the regulation of EPO production, hepcidin expression, and erythropoiesis.