bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of Lysinibacillus sp. Strain A1, Isolated from Malaysian Tropical Soil
Authors: Chan Kok-Gan , Chen Jian Woon , Chang Chien-Yi , Yin Wai-Fong , Chan Xin-Yue 
Published: March 2015

ABSTRACT
In this work, we describe the genome of Lysinibacillus sp. strain A1, which was isolated from tropical soil. Analysis of its genome sequence shows the presence of a gene encoding for a putative peptidase responsible for nitrogen compounds.
Whole-Genome Sequencing of a
Pandoravirus
Isolated from Keratitis-Inducing
Acanthamoeba
Authors: Antwerpen M. H. , Georgi E. , Zoeller L. , Woelfel R. , Stoecker K. , Scheid P. 
Published: March 2015

ABSTRACT

Following the recent discovery of two
Pandoravirus
species in 2013, a previously described endocytobiont isolated from the inflamed eye of a patient with keratitis was subjected to whole-genome sequencing (WGS). Here, we present the complete genome sequence of a new
Pandoravirus
isolate.
Complete Genomic Sequence of Rabies Virus from an Ethiopian Wolf
Authors: Marston Denise A. , Wise Emma L. , Ellis Richard J. , McElhinney Lorraine M. , Banyard Ashley C. , Johnson Nicholas , Deressa Asefa , Regassa Fekede , de Lamballerie Xavier , Fooks Anthony R. , Sillero-Zubiri Claudio 
Published: March 2015

ABSTRACT
Ethiopian wolves are the rarest canid in the world, with only 500 found in the Ethiopian highlands. Rabies poses the most immediate threat to their survival, causing epizootic cycles of mass mortality. The complete genome sequence of a rabies virus (RABV) derived from an Ethiopian wolf during the most recent epizootic is reported here.
Complete Genome Sequence of a Chikungunya Virus Imported from Bali to Germany
Authors: Wölfel Silke , Vollmar Patrick , Poluda David , Zange Sabine , Antwerpen Markus H. , Löscher Thomas , Dobler Gerhard 
Published: March 2015

ABSTRACT
Chikungunya virus (CHIKV) strain DH130003 was isolated from a traveler with Chikungunya fever returning from Bali to Germany. Although strains of the east-central/south African lineage bearing the A226V mutation have predominated in most parts of Asia since 2005, CHIKV DH130003 belongs to the Asian lineage.
Genome Sequence of Vibrio campbellii Strain UMTGB204, a Marine Bacterium Isolated from a Green Barrel Tunicate
Authors: Gan Huan You , Noor Mohd Ezhar Mohd , Saari Nur Azna , Musa Najiah , Mustapha Baharim , Usup Gires , Danish-Daniel Muhd 
Published: March 2015

ABSTRACT

Vibrio campbellii
strain UMTGB204 was isolated from a green barrel tunicate. The genome of this strain comprises 5,652,224 bp with 5,014 open reading frames, 9 rRNAs, and 116 tRNAs. It contains genes related to virulence and environmental tolerance. Gene clusters for the biosynthesis of nonribosomal peptides and bacteriocin were also identified.
Draft Genome of the Multidrug-Resistant Acinetobacter baumannii Strain A155 Clinical Isolate
Authors: Arivett Brock A. , Fiester Steven E. , Ream David C. , Centrón Daniela , Ramírez Maria S. , Tolmasky Marcelo E. , Actis Luis A. 
Published: March 2015

ABSTRACT

Acinetobacter baumannii
is a bacterial pathogen with serious implications on human health, due to increasing reports of multidrug-resistant strains isolated from patients. Total DNA from the multidrug-resistant
A. baumannii
strain A155 clinical isolate was sequenced to greater than 65× coverage, providing high-quality contig assemblies.
Draft Genome Sequences of Pseudomonas fluorescens Strains SF39a and SF4c, Potential Plant Growth Promotion and Biocontrol Agents
Authors: Ly Lindsey K. , Underwood Grace E. , McCully Lucy M. , Bitzer Adam S. , Godino Agustina , Bucci Vanni , Brigham Christopher J. , Príncipe Analía , Fischer Sonia E. , Silby Mark W. 
Published: March 2015

ABSTRACT

Pseudomonas fluorescens
SF4c and SF39a, strains isolated from wheat rhizosphere, have potential applications in plant growth promotion and biocontrol of fungal diseases of crop plants. We report the draft genome sequences of SF4c and SF39a with estimated sizes of 6.5 Mb and 5.9 Mb, respectively.
Complete Genome Sequence of Feline Panleukopenia Virus Strain HRB-CS1, Isolated from a Domestic Cat in Northeastern China
Authors: Liu Chunguo , Liu Yongxiang , Liu Dafei , Qiu Zheng , Tian Jin , Guo Dongchun , Li Zhijie , Liu Ming , Li Yijing , Qu Liandong 
Published: March 2015

ABSTRACT
Here, we report the complete genome sequence of feline panleukopenia virus (FPLV) strain HRB-CS1, isolated from a dead domestic cat showing enteric symptoms in China in 2014. The genome of HRB-CS1 was sequenced and analyzed, which will help to understand the genetic characteristics and evolution of FPLV in China.
Workplace violence against nurses in Chinese hospitals: a cross-sectional survey
Authors: Jiao Mingli , Ning Ning , Li Ye , Gao Lijun , Cui Yu , Sun Hong , Kang Zheng , Liang Libo , Wu Qunhong , Hao Yanhua 
Published: March 2015

ObjectivesTo determine the prevalence of workplace violence that Chinese nurses have encountered, identify risk factors and provide a basis for future targeted interventions.SettingHeilongjiang, a province in northeast China.MethodsA cross-sectional survey.ParticipantsA total of 588 nurses provided data. There were also in-depth interviews with 12 nurses, 7 hospital administrators and 6 health officials.ResultsA total of 7.8% of the nurses reported physically violent experiences and 71.9% reported non-physically violent experiences in the preceding year. Perpetrators were patients or their relatives (93.5% and 82%, respectively), and 24% of nurses experienced non-physical violence that involved Yi Nao (gangs specifically targeting hospitals). Inexperienced nurses were more likely to report physical (13.2%) or non-physical (89.5%) violence compared with experienced nurses. Graduate-level nurses were more likely to perceive and report non-physical violence (84.6%). Nurses who worked rotating shifts were 3.668 times (95% CI 1.275 to 10.554) more likely to experience physical violence, and 1.771 times (95% CI 1.123 to 2.792) more likely to experience non-physical violence compared with nurses who worked fixed day shifts. Higher anxiety levels about workplace violence and work types were associated with violence. Interviewees perceived financial burdens, unsatisfactory treatment outcomes and miscommunications as influencing factors for workplace violence.ConclusionsPreplacement education should focus on high-risk groups to reduce workplace violence. Increased awareness from the public and policymakers is necessary to develop effective control strategies at individual, hospital and national levels.
Cognitive impairment in Parkinson's disease
Authors: Cosgrove Jeremy , Alty Jane Elizabeth , Jamieson Stuart 
Published: March 2015

ABSTRACT
Cognitive impairment is a significant non-motor symptom of Parkinson's disease (PD). Longitudinal cohort studies have demonstrated that approximately 50% of those with PD develop dementia after 10 years, increasing to over 80% after 20 years. Deficits in cognition can be identified at the time of PD diagnosis in some patients and this mild cognitive impairment (PD-MCI) has been studied extensively over the last decade. Although PD-MCI is a risk factor for developing Parkinson's disease dementia there is evidence to suggest that PD-MCI might consist of distinct subtypes with different pathophysiologies and prognoses. The major pathological correlate of Parkinson's disease dementia is Lewy body deposition in the limbic system and neocortex although Alzheimer's related pathology is also an important contributor. Pathological damage causes alteration to neurotransmitter systems within the brain, producing behavioural change. Management of cognitive impairment in PD requires a multidisciplinary approach and accurate communication with patients and relatives is essential.
Topology change due to particle heterogeneity in DLAs
Authors: Candia Lucas Ismael , Carbonetti Julio , Garcia Guillermo Daniel , Sanchez-Varretti Fabricio Orlando 
Published: March 2015

In the present paper, a variation of the widespread model of diffusion-limited aggregation (DLA) is presented. Unlike the traditional DLA model, where particles are attached to the aggregate whenever they touch it, we here restrict attachment by reducing the number of available bonds of the particles. This subtle change in the model changes the topological properties of the resulting aggregate. By using a binary mixture of particles, with different coordination number, the fractal dimension (df), the spectral dimension (ds) and the random walk dimension (dw) are studied as a function of particle-type ratio. The behavior of the system shows non-negligible deviation from the traditional model.
Cucurbitacin B Alters the Expression of Tumor-Related Genes by Epigenetic Modifications in NSCLC and Inhibits NNK-Induced Lung Tumorigenesis
Authors: Shukla Samriddhi , Khan Sajid , Kumar Sudhir , Sinha Sonam , Farhan Mohd. , Bora Himangsu K. , Maurya Rakesh , Meeran Syed Musthapa 
Published: March 2015

Abstract
Non–small cell lung cancer (NSCLC) represents almost 85% of total diagnosed lung cancer. Studies have shown that combination of DNA methyltransferase (DNMT) and histone deacetylase (HDAC) inhibitors is effective against various cancers, including lung cancer. However, optimizing the synergistic dose regime is very difficult and involves adverse side effects. Therefore, in this study, we have shown that cucurbitacin B (CuB), a single bioactive triterpenoid compound, inhibits both DNMTs and HDACs starting at a very low dose of 60 nmol/L in NSCLC H1299 cells. The CuB-mediated inhibition of DNMTs and HDACs in H1299 cells leads to the reactivation of key tumor suppressor genes (TSG) such as CDKN1A and CDKN2A, as well as downregulation of oncogenes c-MYC and K-RAS and key tumor promoter gene (TPG), human telomerase reverse transcriptase (hTERT). The upregulation of TSGs and downregulation of TPG were consistently correlated with the alterations in their promoter methylation and histone modifications. This altered expression of TPG and TSGs is, at least in part, responsible for the inhibition of cellular proliferation and induction of cellular apoptosis in NSCLC. Furthermore, CuB treatment significantly inhibited the tumor incidence and multiplicity in 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK)-induced lung tumorigenesis in A/J mice, which was associated with the induction of apoptosis and inhibition of hyperproliferation in the lung tissues. Together, our study provides new insight into the CuB-mediated epigenetic alterations and its chemotherapeutic effects on lung cancer. Cancer Prev Res; 8(6); 552–62. ©2015 AACR.
Ink4a/Arf-Dependent Loss of Parietal Cells Induced by Oxidative Stress Promotes CD44-Dependent Gastric Tumorigenesis
Authors: Seishima Ryo , Wada Takeyuki , Tsuchihashi Kenji , Okazaki Shogo , Yoshikawa Momoko , Oshima Hiroko , Oshima Masanobu , Sato Toshiro , Hasegawa Hirotoshi , Kitagawa Yuko , Goldenring James R. , Saya Hideyuki , Nagano Osamu 
Published: March 2015

Abstract
Loss of parietal cells initiates the development of spasmolytic polypeptide–expressing metaplasia (SPEM), a precancerous lesion in stomach. CD44 variant (CD44v) that enhances the ability to defend against reactive oxygen species (ROS) in epithelial cells is expressed de novo in SPEM of K19-Wnt1/C2mE mice, a transgenic model of gastric tumorigenesis, and is required for the efficient development of SPEM and gastric tumor in these animals. The role of ROS and its downstream signaling in CD44-dependent gastric tumorigenesis has remained unknown, however. With the use of the K19-Wnt1/C2mE mouse, we now show that parietal cells in the inflamed stomach are highly sensitive to oxidative stress and manifest activation of p38MAPK signaling by ROS. Oral treatment with the antioxidant ascorbic acid or genetic ablation of the Ink4a/Arf locus, a major downstream target of ROS-p38MAPK signaling, inhibited parietal cell loss and the subsequent gastric tumorigenesis. Our results indicate that signaling activated by oxidative stress in parietal cells plays a key role in CD44-dependent gastric tumorigenesis. Cancer Prev Res; 8(6); 492–501. ©2015 AACR.
Histone Methyltransferase SET1 Mediates Angiotensin II–Induced Endothelin-1 Transcription and Cardiac Hypertrophy in Mice
Authors: Yu Liming , Yang Guang , Weng Xinyu , Liang Peng , Li Luyang , Li Jianfei , Fan Zhiwen , Tian Wenfang , Wu Xiaoyan , Xu Huihui , Fang Minming , Ji Yong , Li Yuehua , Chen Qi , Xu Yong 
Published: March 2015

Objective—
Endothelin-1 is a potent vasoconstrictor derived from vascular endothelium. Elevated endothelin-1 levels are observed in a host of cardiovascular pathologies including cardiomyopathy. The epigenetic mechanism responsible for endothelin-1 induction in these pathological processes remains elusive.

Approach and Results—
We report here that induction of endothelin-1 expression in endothelial cells by angiotensin II (Ang II) was accompanied by the accumulation of histone H3K4 trimethylation, a preeminent histone modification for transcriptional activation, on the endothelin-1 promoter. In the meantime, Ang II stimulated the expression and the occupancy of Suv, Ez, and Trithorax domain 1 (SET1), a mammalian histone H3K4 trimethyltransferase, on the endothelin-1 promoter, both in vitro and in vivo. SET1 was recruited to the endothelin-1 promoter by activating protein 1 (c-Jun/c-Fos) and synergized with activating protein 1 to activate endothelin-1 transcription in response to Ang II treatment. Knockdown of SET1 in endothelial cells blocked Ang II–induced endothelin-1 synthesis and abrogated hypertrophy of cultured cardiomyocyte. Finally, endothelial-specific depletion of SET1 in mice attenuated Ang II–induced pathological hypertrophy and cardiac fibrosis.

Conclusions—
Our data suggest that SET1 epigenetically activates endothelin-1 transcription in endothelial cells, thereby contributing to Ang II–induced cardiac hypertrophy. As such, screening of small-molecule compound that inhibits SET1 activity will likely offer a new therapeutic solution to the treatment of cardiomyopathy.
Changes in Wall Shear Stress of Cerebral Arteriovenous Malformation Feeder Arteries After Embolization and Surgery
Authors: Alaraj Ali , Shakur Sophia F. , Amin-Hanjani Sepideh , Mostafa Hassan , Khan Sajeel , Aletich Victor A. , Charbel Fady T. 
Published: March 2015

Background and Purpose—
Wall shear stress (WSS) has been implicated as an important stimulus for vascular remodeling. The purpose of this study is to measure WSS in AVM arterial feeders using quantitative magnetic resonance angiography pre- and post-embolization/surgery.

Methods—
Records of patients who underwent AVM embolization and surgical resection at our institution between 2007 and 2013 and had WSS, flow rate, and vessel diameter obtained pre- and post-treatment using quantitative magnetic resonance angiography were retrospectively reviewed. WSS was compared between the feeder and contralateral artery pre- and post-embolization/surgery.

Results—

Twenty-one patients were included (mean age 34 years, 19% hemorrhagic presentation), with Spetzler–Martin grades 1 to 4. WSS, blood flow, and vessel diameter were assessed in a total of 51 feeder arteries. At baseline, mean WSS was significantly higher compared with the contralateral vessel (29.7±12.0 dynes/cm
2
versus 23.3±11.0 dynes/cm
2
;
P
=0.007). After embolization (23.0 dynes/cm
2
versus 22.5 dynes/cm
2
;
P
=0.78) and surgery (17.9 dynes/cm
2
versus 23.2 dynes/cm
2
;
P
=0.09), WSS was not significantly different than in the contralateral vessel. Reduced WSS post-embolization corresponded to significantly decreased flow (338.1 mL/min versus 170.3 mL/min;
P
<0.001) and smaller vessel diameter (3.7 mm versus 3.5 mm;
P
=0.01).

Conclusions—
Enlargement of cerebral AVM arterial feeders is insufficient to compensate for increased blood flow, creating high WSS. After treatment, flow diminishes and so WSS and vessel diameter concomitantly decrease. Thus, WSS plays a pivotal role in vascular remodeling that may be exploited to monitor AVM response to treatment or understand other high-flow vascular pathologies.
Direct Flux Measurements from Mobile Platforms at Sea: Motion and Airflow Distortion Corrections Revisited
Authors: Landwehr Sebastian , O’Sullivan Niall , Ward Brian 
Published: March 2015

AbstractShip-based measurements of wind speed and direct fluxes are affected by airflow distortion that can lead to a tilt of the wind vector as well as acceleration or deceleration of the wind speed. Direct flux measurements are additionally affected by the fluctuating velocity of the platform. The classic approach is to first correct the wind speed for angular and translational platform velocities and thereafter rotate the wind vector into the mean flow. This study finds that for ships under way, this leads to an overestimation of the vector tilt and biased flux estimates. This may explain the common observation that flux estimates from ships in transit have lower quality than measurements taken on station. Here an alternative approach is presented, where the flow-distortion-induced tilt of the wind vector is estimated from the 3D wind speed measurements and applied to the apparent wind vector. The tilt correction is carried out after correction for the fluctuating part of the platform velocity but before removing the ship’s mean translational velocity. This new method significantly improved the agreement of direct momentum flux measurements made from a ship under way with the parameterization of the COARE3.5 bulk model. The sensitivity of the eddy covariance measurements of momentum and scalar fluxes to the choice of the tilt-motion correction method is analyzed, and this study proposes that a reanalysis of previous direct flux measurements with the new method discussed here can improve researchers’ understanding of air–sea interaction.
Physicians’ Lack of Adherence to National Heart, Lung, and Blood Institute Guidelines for Pediatric Lipid Screening
Authors: Valle Christopher W. , Binns Helen J. , Quadri-Sheriff Maheen , Benuck Irwin , Patel Angira 
Published: March 2015

Objectives. To determine adherence to the 2011 National Heart, Lung, and Blood Institute lipid screening guidelines and identify patient factors promoting screening. Methods. Records of children who received well-child care at age 11 years and turned 12 in 2013 were reviewed. Subjects were stratified by guideline-defined dyslipidemia risk based on documented medical or family history risk factors. We defined adherence as the order of a lipid profile when age 11 years or completed lipid screening at 9 to 10 years. Results Of 298 subjects, 42% were assigned to the dyslipidemia high-risk subgroup. Records of 27.2% demonstrated adherence. Fifty-six percent of high-risk subjects versus 6% of their non-high-risk counterparts received lipid screening by age 12 ( P < .001). Among screened subjects, history of obesity and parental history of dyslipidemia were significantly associated with lipid testing. Conclusions. Lipid screening rates were low. Strategies to increase lipid screening in the primary care setting are needed.
Outcome of patients with systemic lupus erythematosus on chronic dialysis: an observational study of incident patients of the French National Registry 2002–2012
Authors: Levy B , Couchoud C , Rougier J-P , Jourde-Chiche N , Daugas E 
Published: March 2015

Objectives The objective of this article is to describe the outcome (mortality, kidney transplantation) of patients with systemic lupus erythematosus (SLE) on chronic dialysis. Methods The overall and cardiovascular (CV) mortality and access to kidney transplantation were studied in all SLE patients incident on chronic dialysis in France between 2002 and 2012 (REIN registry). They were compared to age- and sex-matched patients with diabetic nephropathy and with autosomal dominant polycystic kidney disease (PKD) on chronic dialysis. Results A total of 368 SLE patients were included in the national REIN registry between 2002 and 2012. Cumulative incidence of death was 16.9% at five years, with no difference between haemodialysis and peritoneal dialysis. Independent risk factors of death were age, past history of cardiovascular disease (CVD) and chronic respiratory insufficiency. At five years, CV and all-cause mortality in SLE patients were lower than in matched diabetic patients, but three-fold higher than in matched PKD patients. Access to the kidney transplant waiting list and to kidney transplantation was higher in SLE patients than in matched diabetic patients, but lower than in matched PKD patients. Conclusions SLE patients on chronic dialysis are a population at high risk of death influenced by CV burden and chronic respiratory failure, but not by the method of dialysis. Their outcome, in terms of mortality and access to kidney transplantation, is intermediate between diabetic patients and patients with PKD.
Topical Review
Authors: Hanselman Andrew E. , Lalli Trapper A. J. , Santrock Robert D. 
Published: March 2015

Fetal tissues are well known for their therapeutic potential. They contain numerous growth factors, cytokines, and matrix components that promote regeneration of tissues while downregulating inflammation and scar formation. As a result, use of these treatments has expanded over the previous 20 years throughout various surgical specialties, including orthopaedics. With improved methods of sterilization, processing, and storage, surgeons need to be informed about the potential benefits of fetal tissue in foot and ankle surgery. The aim of this review is to provide a brief historical background, basic anatomy and physiology, and a current review of the literature in regard to chronic wounds, diabetic foot ulcerations, plantar fasciitis, tendon repair, adhesion prevention, nerve repair, and bone healing. Levels of Evidence: Level V: Expert Opinion
Hyperglycemia repression of miR-24 coordinately upregulates endothelial cell expression and secretion of von Willebrand factor
Authors: Xiang Yaozu , Cheng Jijun , Wang Dandan , Hu Xiaoyue , Xie Yi , Stitham Jeremiah , Atteya Gourg , Du Jing , Tang Wai Ho , Lee Seung Hee , Leslie Kristen , Spollett Geralyn , Liu Zejian , Herzog Erica , Herzog Raimund I. , Lu Jun , Martin Kathleen A. , Hwa John 
Published: March 2015

Key Points
miR-24 tightly regulates VWF expression, maturation, and secretion. Hyperglycemia regulates VWF through aldose reductase, ROS, and c-Myc.