bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow Cytometric Investigations of Corneocytes from Psoriatic Scales and the Effects of Ingram Therapy
Authors: Bauer Franz W. , Boezeman Jan B.M. , de Grood Rufi M. , Koopman Roland J.J. 
Published: April 2015

AbstractFlow cytometry has been used to study the stratum corneum. Following mechanical disruption and propidium iodide staining for DNA, analysis of the fluorescence intensity profile permits discrimination between nucleated and anucleate corneocytes. It was established that the nucleated corneocyte from parakeratotic stratum corneum contains double‐stranded DNA in the normal diploid amount.A model investigation is described following the normalization of the psoriatic stratum corneum during Ingram therapy. It is shown that the initially high percentage of nucleated corneocytes (50%) falls exponentially during the first weeks of therapy. The rate of normalization seems to offer a quantitative and objective approach for evaluation and optimization of therapy.
Reticular Erythematous Mucinosis Syndrome
Authors: Funai Tatsuhiko , Aoki Toshiyuki 
Published: April 2015

AbstractA 71‐year‐old man visited us with asymptomatic erythematous patches of two years' duration on the sternum and right costal arch areas. Histologically there was an appreciable amount of lymphocytic infiltration around the infundibulum of the hair follicle and the dermal capillaries, but the deposition of a colloidal iron positive material was observed around the lymphocytic infiltration and between the collagen bundles to only a mild degree. Reticular erythematous mucinosis (REM) syndrome is controversial; what is its nature as a mucinosis? This case seems to be the extreme of REM syndrome in which mucin deposition was minimal.
Maintenance of Epidermal Structures of Psoriatic Skin in Organ Culture
Authors: Kondo Shigeo 
Published: April 2015

AbstractA method for maintaining the epidermal structures of the normal human and uninvolved and involved psoriatic skin explants in organ culture was described. Skin explants were put in diffusion chambers made of millipore filters, which were then submitted to a roller tube culture at 15 rpm and 32°C. With a gas phase of air + 5% CO2 (phase A), both normal and psoriatic skin explants in culture showed parakeratosis or degeneration of their epidermal cells; with a gas phase of either 50% O2 + 45% N2 + 5% CO2 (phase B) or 95% O2 + 5% CO2 (phase C), they showed neither parakeratosis nor degeneration of epidermal cells and maintained their original structures for 14 days. Autoradiographically, the number of thymidine‐3H (3H‐TdR) labeled epidermal cells in organ culture was greater in involved psoriatic skin explants than in uninvolved ones. Isoproterenol‐stimulated cyclic AMP (cAMP) levels were lower in involved psoriatic epidermis of skin explants than in uninvolved ones, and plasminogen activator activity was higher in the former than in the latter. Addition of dexamethasone normalized the keratinization of the involved psoriatic explant in culture, but addition of dibutyryl cyclic AMP (db cAMP) and dibutyryl cyclic GMP (db cGMP) did not.
Interrelationship between Biliary Dyskinesia and Chronic Urticaria, Chronic Eczema and Atopic Dermatitis
Authors: Nishimura Toshio 
Published: April 2015

AbstractInterrelationships between biliary dyskinesia and chronic urticaria, chronic eczema and atopic dermatitis have been studied in 210 biliary dyskinesia patients and 60 normal subjects. Incidences of chronic urticaria, chronic eczema and atopic dermatitis were found more frequently in biliary dyskinesia patients than normal subjects.Skin symptoms of these chronic urticaria, chronic eczema and atopic dermatitis cases showed significant normalization with improved gallbladder contraction rate. COMT inhibitor therapy and fat restriction were more effective for the skin symptoms and for prolonging the interval before recidivation or exacerbation of these allergic diseases than conservative therapy.
Eccrine Tubular Adenoma —A Histopathological, Histochemical, and Ultrastructural Study—
Authors: Oka Keiko , Katsumata Michio 
Published: April 2015

AbstractA case of eccrine tubular adenoma on the dorsum of the right foot is presented. Histopathologically, in the central nodule of the tumor, the whole dermis was involved and the tumor islands were connected to the epidermis; in the rest of the lesion, tumor islands were observed in the upper dermis. The tumor islands were composed of cystic or alveolar structures and cell masses possessing multiple lumina. Tubules were surrounded by two or more layers of epithelium forming papillations projecting into the lumen. There were only a few basaloid islands and no sclerotic strands. The tumor cells were well differentiated. Acid mucopolysaccharides were seen in the stroma. Histochemically, phosphorylase and acid phosphatase reacted moderately. Succinic dehydrogenase gave a weak reaction and β‐glucuronidase was negative in tumor cells. Ultrastructurally, intracellular ducts with numerous microvilli, periluminal filamentous zones, and many multivesicular dense bodies surrounded by a limiting membrane were observed. Keratohyalin granules were absent. Based on these findings, the tumor reported here was considered to be an adenoma differentiating toward the eccrine duct. Some aspects resembled tubular apocrine adenoma, syringoma and basal cell tumor with eccrine differentiation, but they were most similar to papillary eccrine adenoma.
Donperidone‐Induced Systemic Lupus‐Like Syndrome
Authors: Yasue Takashi , Nitta Yukiko 
Published: April 2015

AbstractIn a 64‐year‐old woman, fever, malar rash, widespread macular erythema, polyarthritis, generalized myalgia, and a positive reaction for antinuclear antibody developed while she was taking donperidone (30 mg daily). Proteinuria and hypocomplementemia were not found. The histopathology of cutaneous lesions was almost consistent with that of systemic lupus erythematosus. The lupus band test on the involved skin was weakly positive, but electron microscopy revealed no microtubular structure in the vascular endothelium of the involved skin.After withdrawal of the drug, there was complete resolution of the clinical signs and symptoms. Resumption of the therapy with the drug resulted in recurrence of her symptoms. The patient's antinuclear antibody reaction was weakly positive over a three‐month follow‐up period.To our knowledge, this is the first reported case of a clinical systemic lupus‐like syndrome induced by therapy with donperidone.
Eczema Herpeticum in Three Siblings: Clinical Features and Acyclovir Treatment
Authors: Chen Yao‐Ping , Wu Ying‐Chin 
Published: April 2015

AbstractThree atopic siblings with sequential primary herpes simplex virus (HSV) infection on their eczematous lesions following their father's recurrent herpes labialis were presented. Eczema herpeticum was diagnosed on the basis of their characteristic grouped umbilicated vesicles superimposed on pre‐existing atopic eczematous lesions. HSV type 1 was identified from the three patients' Tzanck smears and viral isolation of vesicular contents by monoclonal antibodies. The skin lesions faded spontaneously in the first case, although this elder brother did not receive any antiviral treatment. Later, he developed mild recurrence of skin lesions on his extremities. His younger twin sisters were treated with systemic acyclovir and no recurrences occurred during 2 years of follow up. All of them developed anti‐HSV antibody about 7 to 14 days after clinical onset. Acyclovir seems to be an effective antiviral agent in treating eczema herpeticum and reducing subsequent recurrences after primary herpes simplex virus infection.
Fine Structural Deformation of the Dermal Capillary Following Immersion Fixation Procedure
Authors: Imayama Shuhei , Urabe Harukuni 
Published: April 2015

AbstractThe possibility of fine structural deformation related to skin biopsy and the subsequent immersion fixation procedure were investigated, because little attention has yet been focused on artifacts of the dermal microvasculature.Contraction of the material following biopsy removal was marked in skin regions with thin epidermis and resulted in capillary collapse. As the collapse of the vessels increased, lining cells became thicker and more rugged, endothelial fenestrations disappeared, and 10 nm filaments aggregated. Simultaneously, perivascular connective tissue material was separated from the dermal element in which the vessels were embedded and appeared as a homogenous areola around the endothelial tube. Basal lamina appeared folded and partially multilaminated around the vascular circumference, particularly in the venous segment of the microvasculature; these are considered to be the definite characteristics of dermal capillaries. In contrast to the skin regions with thin epidermis, the vessels in “well developed” dermal papillae did not collapse and bore close similarities to the perfused ones. The present study indicates that most of the so‐called characteristics of dermal capillaries in biopsy skin is attributable to artifacts following the removal of the skin before fixation.
Staphylococcal Scalded Skin Syndrome in an Adult
Authors: Takiuchi Iwao , Sasaki Hiromi , Takagi Hisae , Higuchi Dousei 
Published: April 2015

AbstractWe report a case of a 77‐year‐old man with staphylococcal scalded skin syndrome (SSSS). The patient, who had no physical abnormalities other than herpes zoster, showed intact humoral and cellular immunity. A 3‐ and 1‐phage group strain of Staphylococcus aureus was isolated from an unruptured pustule of the herpes zoster lesion, but not from the throat. Inoculation of the isolated bacterium into newborn mice produced a characteristic Nikolsky sign. Histological diagnosis of SSSS was established by findings of intraepithelial cleavage through the stratum granulosum and a lack of inflammatory cells in both dermis and epidermis. After a ten‐day course of oral cefaclor (250 mg every 8 hours) and topical triamcinolone acetonide containing fradiomycin and gramycidin J, the patient recovered completely. The patient left the hospital without any scars other than those of herpes zoster.
Febrile Ulceronecrotic Mucha‐Habermann's Disease and Its Successful Therapy with DDS
Authors: Nakamura Shinichi , Nishihara Kiyoshi , Nakayama Keiji , Hoshi Kenji 
Published: April 2015

AbstractA case of febrile ulceronecrotic Mucha‐Habermann's disease and its therapy with 4,4‐diaminodiphenyl sulfone (DDS) are reported. This disorder is a severe form of pityriasis lichenoides et varioliformis acuta (PLEVA) characterized by the sudden eruption of diffuse coalescent ulcerations associated with systemic symptoms. In our case, the disease was preceded by the mild form of PLEVA and the patient was admitted with diffuse ulceronecrotic eruptions and high fever. After admission, the patient, who had not responded to antibiotics, recovered dramatically with DDS therapy.
Senile Comedones Which Developed away from Xanthelasmas on Periorbital Regions
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractA case of senile comedones in association with xanthelasmas which developed on the periorbital regions of an elderly male is described. Since, in this instance, the comedones developed away from xanthelasmas, an association between the two disorders on the periorbital regions different from that described in any other report is suggested. Previously, comedones have always been confined to xanthelasmas.
A Novel Protein of Rat Epidermis —Haematoxylin Stain‐Positive Protein of Keratohyalin Granules as the Membrane Lining Protein of the Stratum Corneum—
Authors: Tezuka Tadashi , Takahashi Masae 
Published: April 2015

AbstractA novel protein, haematoxylin stain‐positive, non‐nucleoprotein has been extracted from the epidermis of 3‐day‐old rats. This protein was purified through preparative isoelectric focusing, followed by sodium dodecylsulfate polyacrylamide gel electrophoresis. The purified protein had a molecular weight of 14,500 daltons and an isoelectric point of 4.7. The amino acid composition included 15% glycine, 11% serine, 13% glutamic acid and 10% aspartic acid, but only 1.9% histidine.Using the immunofluorescent and immunoelectron microscopic techniques, observation showed that the antibody formed as a result of purified antigen was located on keratohyalin granules and in the cell membrane of the stratum corneum of the skin.This haematoxylin stain‐positive protein could be synthesized in the stratum granulosum as one component of keratohyalin granules, coat the stratum corneum cell membrane and perhaps, we think, may act as one important component of the epidermal barrier.
Surface Densities of Murine Ia + Dendritic Epidermal Cells (Ia + DECs) and Thy‐1 + Dendritic Epidermal Cells (Thy‐1 + DECs) in Relationship to Aging and Ultraviolet B (UVB) Radiation
Authors: Gu Shao‐Qiu , Sakuma Mariko , Naito Shuichi , Baba Toru , Uyeno Ken‐ichi 
Published: April 2015

AbstractIdentification and enumeration of both Ia + dendritic epidermal cells (Ia + DECs) and Thy‐1 + dendritic epidermal cells (Thy‐1 + DECs) from various parts of the body and non‐irradiated and ultraviolet B (UVB) irradiated back skin were examined using epidermal sheets of C3H/He inbred mice of different age groups and indirect immunofluorescent technique. The following results were obtained: [1] There was a significant decline in both Ia + DEC and Thy‐1 + DEC density in the mice in the oldest group (48–50 weeks); [2] The densities of Ia + DECs were significantly higher than those of Thy‐1 + DECs in comparisons of various parts of the body; [3] At 24 h after 60–120 mJ/cm2 UVB irradiation, the Ia + DECs and Thy‐1 + DECs decreased significantly in a dose‐dependent fashion. The Ia + DECs decreased drastically (p<0.01) while the Thy‐1 + DECs decreased mildly (p<0.05). [4] The degree or resistance to UVB differed between Ia + DECs and Thy‐1 + DECs in older mice (40–48 weeks). These findings may imply that the decline of the Ia + DECs and Thy‐1 + DECs reflects alterations in immune response during aging; As do Ia + DECs, Thy‐1 + DECs might also play a role in UVB induced specific unresponsiveness in contact hypersensitivity; each type of Ia + DECs and Thy‐1 + DECs follows a distinct biological kinetic pattern after UVB irradiation.
Familial Benign Chronic Pemphigus Generalized by Pseudomonas Infection
Authors: Takami Yoshihiro , Akutsu Yutaka , Suzuki Masako , Takahashi Makoto 
Published: April 2015

AbstractWe report a case of generalized familial benign chronic pemphigus (FBCP) whose generalized condition was induced by an infection by Pseudomonas aeruginosa. A 34‐year‐old male suffered erythemato‐macerative lesions over most of his body. Upon microbiological examination, Pseudomonas aeruginosa was cultured from the pus of the lesions. Histologically, a specimen from the abdominal lesion revealed typical features of familial benign chronic pemphigus. It is well known that FBCP is exacerbated by topical infection. We supposed that, in this case, the infection by Pseudomonas aeruginosa was induced by inappropriate therapy with cortiosteroid. Topical application of silversulfadiazin cream was effective in improving the generalized lesions of this condition.
Transition of Isolation Ratios of Dermatophytes from House Dust of Patients with Tinea
Authors: Shirouchi Yoko , Murata Joji 
Published: April 2015

AbstractHouse dust samples from 7 families of 13 patients with tinea due to T. mentagrophytes (5 patients), M. gypseum (1 patient) and M. cants (6 patients) were cultured several times during their clinical courses. In all cases, the numbers of colonies and positive culture plates of the etiologic dermatophytes decreased in number during tinea treatment. T. mentagrophytes was not isolated from house dust any more by the time the patients were cured; M. canis was still isolated after the tinea lesions had healed. These results seem to indicate: 1) cat fur, the source of infection, is difficult to remove by simple cleaning of the house (or clothing). 2) The parasitic form of the fungus is much more resistant and the numbers of dermatophytes in the fur are greater than those in the scales of the patients.
Increased Densities of Langerhans Cells in the Epidermis of Skin Tumors
Authors: Yamaji Kazuhiko , Matsui Masahiko , Saida Toshiaki 
Published: April 2015

AbstractUsing OKT6 monoclonal antibody (MCA) and β‐specific anti‐S‐100 protein (S‐100β) MCA, the numbers of Langerhans cells (LC) in the epidermis overlying squamous cell carcinoma (SCC), basal cell epithelioma (BCE), dermatofibroma (DF), and in the lesional epidermis of seborrheic keratosis (SK) were investigated. The numbers of LC were significantly increased in the epidermis overlying SCC and in SK. Moreover, significantly more LC were observed in the epidermis overlying SCC than in SK. The increased density of LC in the epidermis overlying SCC may suggest participation and activation of the mononuclear phagocyte system in this neoplasm. In 8 cases of SCC, the subpopulations of infiltrating lymphocytes around the tumor cell nests were analyzed. The ratio of Leu 3a‐positive cells/Leu 2a‐positive cells ranged from 0.63 to 1.99. This diversity may reflect complicated immunological interaction between the tumors and the hosts.
PCB and PCQ Concentrations in Subcutaneous Tissue from Patients with PCB Poisoning (Yusho)
Authors: Ohgami Taro , Nonaka Shigeo , Watanabe Masahisa , Yamashita Kazunori , Murayama Fumio , Yoshida Hikotaro , Masuda Nobuhiro 
Published: April 2015

AbstractPolychlorinated biphenyl (PCB) and polychlorinated quaterphenyl (PCQ) concentrations in the blood and subcutaneous fat tissue from 11 patients with PCB poisoning (Yusho) and 10 controls were analyzed by electron captured gaschromatography. The mean concentration levels of blood PCB and PCQ and the CB% ratio were elevated in patients with Yusho. The PCB pattern was the A type in 5 cases and the B type in 6 others. The mean concentration level of PCB in subcutaneous fat tissue was 1579.45 ppb in the Yusho group, 3.9 times higher than that in the control group. The mean concentration level of PCQ was 206.65 ppb, 119 times higher than that in the control group. The mean value of CB% was 3.22 in the Yusho group and 0.66 in the control group. These results show that PCB and PCQ accumulate in the subcutaneous fat tissue of patients with PCB poisoning (Yusho), as late as 17 years after diagnosis of the disease. The PCB and PCQ concentration levels in the subcutaneous fat tissue appear to be useful in diagnosis and evaluation of prognosis in this disease. Investigations of the elimination of PCB and PCQ in subcutaneous tissues should be done.
Enhancement of Monocyte Chemotactic Activity in the Sera of Psoriatic Patients after Heat Treatment
Authors: Isoda Midori , Hidaka Keiko 
Published: April 2015

AbstractThe chemotactic activity for neutrophils and monocytes was examined in the sera of psoriatic patients and healthy controls using a Boyden chamber method. There were no significant differences in neutrophil chemotactic activity between the sera of psoriatic patients and healthy controls. However, the monocyte chemotactic activity of psoriatic serum was lower than that of control serum. Furthermore, there was a significant increase in the monocyte chemotactic activity of psoriatic sera after heat treatment (42°C, 1 hr).These results suggest that psoriatic serum has a defect of monocyte chemotaxis which can be corrected, in part, by heat treatment.
A Novel Protein of Rat Epidermis II — The Acidic, Histidine‐rich Protein as the Membrane Lining Protein of the Stratum Corneum —
Authors: Takahashi Masae , Tezuka Tadashi 
Published: April 2015

AbstractA novel, acidic histidine‐rich protein, has been extracted from the epidermis of 3‐day‐old rats. This protein was purified through preparative isoelectric focusing, followed by sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS‐PAGE). The purified protein had a molecular weight of 56,000 daltons and an isoelectric point of 5.1. The amino acid composition included 17% glycine, 11% glutamic acid, 10% alanine, 4% histidine and 3% tyrosine.Using the immunofluorescent technique, observation showed that the antibody formed as a result of purified antigen was located in the cell membrane of the stratum corneum of the skin.This acidic, histidine‐rich protein could coat the stratum corneum cell membrane and perhaps act as one important component of the epidermal barrier.
Localized Pemphigoid
Authors: Sugihara Kumiko , Dekio Satoshi , Tohgi Kimiko , Jidoi Joji 
Published: April 2015

AbstractIn this paper, we describe a 69‐year‐old Japanese woman with localized pemphigoid. Direct immunofluorescence study showed linear IgG, IgA and C3 deposits at the basement membrane zone (BMZ). In the serum, circulating anti‐BMZ antibodies of both IgG and IgA classes were found. This is the first report of a patient with localized pemphigoid in whom circulating anti‐BMZ antibodies of not only the IgG class, but also of the IgA class were present.
Regeneration and Degradation of Basal Lamina‐associated Anionic Sites In vitro
Authors: Manabe Motomu 
Published: April 2015

AbstractThe emergence of anionic sites during basement membrane zone formation was studied using migrating epidermis in organ culture as a model system. Ultrastructural investigations using a strongly cationized probe revealed that the anionic sites were found to be formed synchronously with the newly built basal lamina after 7 days in culture.Additionally, we examined the effect of chemically generated hydroxyl radicals on basal lamina‐associated anionic sites. Cytochemical studies showed that hydroxyl radicals produced a loss of cationic tracer positive anionic particles; this effect was inhibited by a specific scavenger, thiourea. These data might suggest that anionic sites were degraded by leukocyte‐derived hydroxyl radicals during acute inflammation near the dermal epidermal junction, resulting in disturbance of the charge‐selective permeability barrier.
Effects of In vitro PUVA Treatment on Adenylate Cyclase Responses of Epidermis
Authors: Kajita Satoshi , Iizuka Hajime 
Published: April 2015

AbstractWe investigated the effect of 8‐methoxypsoralen (8‐MOP) plus long wave ultraviolet irradiation (PUVA) on the pig skin epidermal cyclic AMP (cAMP) system. Following PUVA treatment in vitro, pig skin squares were incubated in RPMI 1640 medium, and the cAMP accumulation by various adenylate cyclase stimulators was determined. A significant increase of the beta‐adrenergic adenylate cyclase response was observed as early as 6‐h following PUVA treatment; the maximal effect was reached at 24‐h and lasted at least for 72‐h. This augmentation effect of PUVA treatment was potentiated by increasing the 8‐MOP concentration (0.5–160 μg/ml) and UVA irradiation dose (0.05–1.4 J/cm2). At higher irradiation doses (2.1–2.8 J/cm2), the beta‐adrenergic augmentation effect was less marked. There were no significant differences in the adenosine‐and histamine‐adenylate cyclase response up to 1.4 J/cm2 irradiation; however, the latter was decreased at a higher irradiation dose (2.8 J/cm2). Although UVA irradiation alone had no effect on the beta‐adrenergic response, 8‐MOP treatment alone increased this receptor response at higher concentrations; this effect was much weaker than that induced by PUVA treatment. Cyclic AMP phosphodiesterase activities were not affected by PUVA treatment. These results indicate that epidermal adenylate cyclase response is affected by in vitro PUVA treatment.