bluuebunny/crossref_metadata_2025_split huggingface dataset:
Central role of FaGAMYB in the transition of the strawberry receptacle from development to ripening
Authors: Vallarino José G. , Osorio Sonia , Bombarely Aureliano , Casañal Ana , Cruz‐Rus Eduardo , Sánchez‐Sevilla José F. , Amaya Iraida , Giavalisco Patrick , Fernie Alisdair R. , Botella Miguel A. , Valpuesta Victoriano 
Published: May 2015

Summary

The receptacle of the strawberry (Fragaria × ananassa) fruit accounts for the main properties of the ripe fruit for human consumption. As it ripens, it undergoes changes similar to other fruits in sugar : acid ratio, volatile production and cell wall softening. However, the main regulators of this process have not yet been reported. The white stage marks the initiation of the ripening process, and we had previously reported a peak of expression for a FaGAMYB gene.

Transient silencing of FaGAMYB using RNAi and further determination of changes in global gene expression by RNAseq, and composition of primary and secondary metabolites have been used to investigate the role played by this gene during the development of the receptacle.

Down‐regulation of FaGAMYB caused an arrest in the ripening of the receptacle and inhibited colour formation. Consistent with this, several transcription factors associated with the regulation of flavonoid biosynthetic pathway showed altered expression. FaGAMYB silencing also caused a reduction of ABA biosynthesis and sucrose content. Interestingly, exogenous ABA application to the RNAI‐transformed receptacle reversed most defects caused by FaGAMYB down‐regulation.

The study assigns a key regulatory role to FaGAMYB in the initiation of strawberry receptacle ripening and acting upstream of the known regulator ABA.
Hemophagocytic lymphohistiocytosis following dengue hemorrhagic fever in Hb H/Hb Constant Spring patient
Authors: Phuakpet Kamon , Sanpakit Kleebsabai , Vathana Nassawee , Takpradit Chayamon , Chokephaibulkit Kulkanya , Viprakasit Vip 
Published: May 2015

AbstractInfection‐associated hemophagocytic syndrome (IAHS), a secondary form of hemophagocytic lymphohistiocytosis (HLH), has been found following several types of infections and can be fatal. We report herein a case of IAHS following dengue infection in a 14‐year‐old patient with underlying α‐thalassemia syndrome (non‐deletional Hb H/Hb Constant Spring disease). He developed prolonged fever, thrombocytopenia, and progressive splenomegaly. Further investigations indicated hyperferritinemia, and increased reactive histiocytes with hemophagocytic activity in the bone marrow. He responded promptly to dexamethasone and i.v. immune globulin. Physicians should be aware of this condition, especially in countries where both dengue hemorrhagic fever and thalassemia are prevalent. The fatal outcome of IAHS can be prevented with prompt appropriate treatment.
The Synthesis and Photocatalytic Properties of TiO2 Nanotube Array by Starch‐Modified Anodic Oxidation
Authors: Zhang Fengjun , Liu Zijian , Lu Wei , Lyu Cong , Lyu Chuan , Wang Xiansheng 
Published: May 2015

AbstractIn this study, the characterization and photocatalytic activity of TiO2 nanotube arrays prepared by anodization process with starch addition were investigated in detail. The results suggested that the optimum mass fraction of starch added in anodization process was 0.1%, with which TiO2 nanotube arrays owning good tubular structure were synthesized. The tube length and average inner diameter of nanotubes were approximately 4 μm and 30 nm, respectively. Through the characterization of TiO2 nanotube arrays by energy dispersive spectrometer, scanning electron microscopy, transmission electron microscopy, X‐ray diffraction, Fourier Transform Infrared (FTIR) spectroscopy, it was found that the as‐prepared nanotubes possessed well uniformed and higher photodegradation responsive than the pure TiO2. Moreover, it was expected that the as‐prepared nanotubes exhibited good photocatalytic activity for the degradation of RhB under UV‐light irradiation, which could be ascribed to their good morphology, enhanced UV‐light absorption property and electron transmission ability during the photocatalytic reaction. In addition, the nanotubes were not significantly regenerated during the cycling runs experiment. Overall, this study could provide a principle method to synthesize TiO2 nanotube arrays with enhanced photocatalytic activity by anodization process with starch addition for environmental purification.
Genetic diversity and structure of Hemileia vastatrix populations on Coffea spp.
Authors: Cabral P. G. C. , Maciel‐Zambolim E. , Oliveira S. A. S. , Caixeta E. T. , Zambolim L. 
Published: May 2015

Coffee leaf rust is the most limiting disease for coffee cultivation in Brazil. Despite its importance, relatively little is known about the genetic diversity of Hemileia vastatrix, the rust causal agent. In this work, the DNA from 112 monopustule isolates from different geographic locations and coffee genotypes were analysed by amplified fragment length polymorphisms (AFLP). The objectives were to assess the influence of the host and geographic origin on the diversity and population differentiation in H. vastatrix. The fungal population showed a low level of genotypic diversity. Gene diversity (h) was 0·027 and the hypothesis of random mating in the total population was rejected, but evidence for recombination was found for two subpopulations (São Paulo and Paraná). The analysis of molecular variance revealed that 90% of the genetic distribution of the pathogen occurs among isolates within the subpopulation (states or host of origin). There was no correlation between geographic and genetic distance (r = −0·024, P = 0·74), which together with the high number of migrants and the low degree of differentiation in populations of H. vastatrix, is consistent with the fact that the inoculum is probably easily dispersed by wind over long distances, allowing dispersal of the pathogen among coffee growing areas in Brazil. Therefore, it is difficult to predict the durability of resistant sources to coffee rust. The recommendation for the breeding programmes is thus to incorporate multigenic resistance as a control strategy.
Optogenetic excitation of preBötzinger complex neurons potently drives inspiratory activity in vivo
Authors: Alsahafi Zaki , Dickson Clayton T. , Pagliardini Silvia 
Published: May 2015

Key points
This study investigates the effects on ventilation of an excitatory stimulus delivered in a spatially and temporally precise manner to the inspiratory oscillator, the preBötzinger complex (preBötC).
We used an adeno‐associated virus expressing channelrhodopsin driven by the synapsin promoter to target the region of the preBötC.
Unilateral optogenetic stimulation of preBötC increased respiratory rate, minute ventilation and increased inspiratory modulated genioglossus muscle activity.
Unilateral optogenetic stimulation of preBötC consistently entrained respiratory rate up to 180 breaths min–1 both in presence of ongoing respiratory activity and in absence of inspiratory activity.
Unilateral optogenetic stimulation of preBötC induced a strong phase‐independent Type 0 respiratory reset, with a short delay in the response of 100 ms.
We identified a refractory period of ∼200 ms where unilateral preBötC optogenetic stimulation is not able to initiate the next respiratory event.
AbstractUnderstanding the sites and mechanisms underlying respiratory rhythmogenesis is of fundamental interest in the field of respiratory neurophysiology. Previous studies demonstrated the necessary and sufficient role of preBötzinger complex (preBötC) in generating inspiratory rhythms in vitro and in vivo. However, the influence of timed activation of the preBötC network in vivo is as yet unknown given the experimental approaches previously used. By unilaterally infecting preBötC neurons using an adeno‐associated virus expressing channelrhodopsin we photo‐activated the network in order to assess how excitation delivered in a spatially and temporally precise manner to the inspiratory oscillator influences ongoing breathing rhythms and related muscular activity in urethane‐anaesthetized rats. We hypothesized that if an excitatory drive is necessary for rhythmogenesis and burst initiation, photo‐activation of preBötC not only will increase respiratory rate, but also entrain it over a wide range of frequencies with fast onset, and have little effect on ongoing respiratory rhythm if a stimulus is delivered during inspiration. Stimulation of preBötC neurons consistently increased respiratory rate and entrained respiration up to fourfold baseline conditions. Furthermore, brief pulses of photostimulation delivered at random phases between inspiratory events robustly and consistently induced phase‐independent (Type 0) respiratory reset and recruited inspiratory muscle activity at very short delays (∼100 ms). A 200 ms refractory period following inspiration was also identified. These data provide strong evidence for a fine control of inspiratory activity in the preBötC and provide further evidence that the preBötC network constitutes the fundamental oscillator of inspiratory rhythms.
Draft Genome Sequences of
Clostridium
Strains Native to Colombia with the Potential To Produce Solvents
Authors: Rosas-Morales Juan Pablo , Perez-Mancilla Ximena , López-Kleine Liliana , Montoya Castaño Dolly , Riaño-Pachón Diego Mauricio 
Published: May 2015

ABSTRACT

Genomes from four
Clostridium
sp. strains considered to be mesophilic anaerobic bacteria, isolated from crop soil in Colombia, with a strong potential to produce alcohols like 1,3-propanediol, were analyzed. We present the draft genome of these strains, which will be useful for developing genetic engineering strategies.
Genome Sequence of Cronobacter sakazakii Serogroup O:4, Sequence Type 4 Strain CDC 2009-03746, Isolated from a Fatal Case of Infantile Meningitis
Authors: Grim Christopher J. , Gopinath Gopal R. , Jarvis Karen G. , Sathyamoorthy Venugopal , Trach Larissa H. , Chase Hannah R. , Tall Ben D. 
Published: May 2015

ABSTRACT

We report the draft genome sequence of a
Cronobacter sakazakii
serogroup O:4, sequence type 4 strain, CDC 2009-03746 (=NM1240=2009-06-01), isolated from a fatal case of infantile meningitis. The draft genome has a size of 4,492,904 bp and a G+C% content of 56.7.
Genome Sequence of Bacillus coagulans P38, an Efficient Polymer-Grade
l
-Lactate Producer from Cellulosic Substrates
Authors: Peng Lili , Song Lifu , Sun Lifan , Cai Yumeng , Wang Limin , Yu Bo 
Published: May 2015

ABSTRACT

Bacillus coagulans
P38 is an efficient polymer-grade
l
-lactic acid producer from a cellulosic carbon source. Here, the draft 3.37-Mb genome sequence of this potential strain may provide useful information to further improve the strain performance for higher titers and, importantly, to understand the mechanism of its high tolerance for 2-furfural.
De Novo
Genome Assembly of Ryegrass Mosaic Virus from a Ryegrass Transcriptome
Authors: Jo Yeonhwa , Choi Hoseong , Cho Won Kyong 
Published: May 2015

ABSTRACT

The ryegrass mosaic virus (RgMV) is a single positive-strand RNA virus belonging to the genus
Rymovirus
. The major natural hosts for RgMV are members of the
Gramineae
species, including ryegrass. Here, we report the nearly complete genome sequence of RgMV by
de novo
assembly using ryegrass transcriptomes.
Draft Genome Sequence of a Sulfur-Oxidizing Autotroph,
Sulfuricella
sp. Strain T08, Isolated from a Freshwater Lake
Authors: Watanabe Tomohiro , Kojima Hisaya , Fukui Manabu 
Published: May 2015

ABSTRACT

Sulfuricella
sp. strain T08 is a sulfur-oxidizing autotroph newly isolated from a freshwater lake in Japan. Strain T08 is the second isolate of the genus
Sulfuricella
. Here, we report the annotated draft genome sequence of the isolate.
Scope and Organization of This Volume
Authors: Spamer Earle E. 
Published: May 2015

Abstract
For various taxonomic names, principally genera and species, this volume piovides information on their location in the literature, published records of stratigraphic occurrence, and published records of geographic occurrence. Information or higher systematics is provided throughout, as an aid for users who may not be familiar with some of the taxa.
The data presented in this volume encompass all publications that in some way address the paleontology of the immediate Grand Canyon region. If a publication has been overlooked, it was not seen by the author. The indexes were prepared from a computerized database, GCPALEO, maintained by the author. This database, at the time these indexes were prepared, contained nearly 17,000 records with taxonomic, stratigraphic, and locality data for fossils reported in the Grand Canyon regional literature.Occurrences of taxonomic names are recorded for every page through the entire base of paleontological literature for the Grand Canyon region. Supplementary databases include higher systematic and stratigraphic data. Aspects of the database allow thorough literature searches at levels of precision greater thaai those of the generalized indexes that comprise this volume. The database includesinformation with each entry that pertains to the context in which the taxon is mentioned; for example, "biostratigraphy," "paleoecology," "mentioned only in a species list," or "casual mention of name."
In this volume, genera and species are cited as published. wunou: synonymy, to preservethe historical context of systematics. Stratigraphic and locality data are modified to reflectmodem names to provide easy use of the indexes, but care also
A pragmatic randomised controlled trial of an implementation intervention to increase healthy eating and physical activity-promoting policies, and practices in centre-based childcare services: study protocol
Authors: Finch Meghan , Yoong Sze Lin , Thomson Rebecca J , Seward Kirsty , Cooney Mairead , Jones Jannah , Fielding Alison , Wiggers John , Gillham Karen , Wolfenden Luke 
Published: May 2015

BackgroundPromotion of healthy eating and physical activity in early childhood is recommended as a global chronic disease prevention strategy. Centre-based childcare services represent a promising setting to provide children with opportunities to improve healthy eating and physical activity. Evidence to inform implementation of childcare obesity prevention guidelines into routine practice in childcare, however, is lacking. This study aims to assess the effectiveness of an intervention, delivered to childcare staff, aiming to increasing service implementation of healthy eating and physical activity-promoting policies and practices.Methods and analysisA pragmatic parallel group randomised controlled trial will be undertaken with 165 childcare services in the Hunter New England region of New South Wales, Australia. Services will be randomised to receive either a 10-month evidence-based performance review intervention with other resources to support practice change, or to a waitlist control group. The primary trial outcome is the proportion of services implementing all of the following recommended healthy eating and physical activity promoting practices: written nutrition, physical activity and small screen recreation policies; providing information to families regarding healthy eating (including breastfeeding), physical activity and small screen time; providing twice weekly healthy eating learning experiences to children; providing water and only plain milk to children; providing fundamental movement skills activities for children every day; and limiting and using electronic screen time more for educational purposes and learning experiences. Effectiveness will be assessed using a telephone interview of practice implementation with childcare staff at baseline and 12 months following baseline.Ethics and disseminationThe study was approved by the Hunter New England Human Research Ethics Committee and the University of Newcastle Human Research Ethics Committee. Study findings will be disseminated widely through peer-reviewed publications and conference presentations.Trial registration numberAustralian New Zealand Clinical Trials Registry ACTRN12614000972628.
The dose–response association of urinary metals with altered pulmonary function and risks of restrictive and obstructive lung diseases: a population-based study in China
Authors: Feng Wei , Huang Xiji , Zhang Ce , Liu Chuanyao , Cui Xiuqing , Zhou Yun , Sun Huizhen , Qiu Gaokun , Guo Huan , He Meian , Zhang Xiaomin , Yuan Jing , Chen Weihong , Wu Tangchun 
Published: May 2015

ObjectiveReduced pulmonary function is an important predictor of environment-related pulmonary diseases; however, evidence of an association between exposures to various metals from all possible routes and altered pulmonary function is limited. We aimed to investigate the association of various metals in urine with pulmonary function, restrictive lung disease (RLD) and obstructive lung disease (OLD) risks in the general Chinese population.DesignA cross-sectional investigation in the Wuhan cohort population.SettingA heavily polluted Chinese city.ParticipantsA total of 2460 community-living Chinese adults from the Wuhan cohort were included in our analysis.Main outcome measuresSpirometric parameters (FVC, forced vital capacity; FEV1, forced expiratory volumes in 1 s; FEV1/FVC ratio), RLD and OLD.ResultsThe dose–response associations of pulmonary function, and RLD and OLD, with 23 urinary metals were assessed using regression analysis after adjusting for potential confounders. The false discovery rate (FDR) method was used to correct for multiple hypothesis tests. Our results indicated that there were positive dose–response associations of urinary iron with FEV1and FEV1/FVC ratio, vanadium with FEV1, and copper and selenium with FEV1/FVC ratio, while a negative dose–response association was observed between urinary lead and FEV1/FVC ratio (all p<0.05). After additional adjusting for multiple comparisons, only iron was dose dependently related to FEV1/FVC ratio (FDR adjusted p<0.05). The dose–response association of iron and lead, with decreased and increased chronic obstructive pulmonary disease risk, respectively, was also observed (both p<0.05). Additionally, we found significant association of urinary zinc with RLD and interaction effects of smoking status with lead on FEV1/FVC, and with cadmium on FVC and FEV1.ConclusionsThese results suggest that multiple urinary metals are associated with altered pulmonary function, and RLD and OLD prevalences.
How different countries addressed the sudden growth of e-cigarettes in an online tobacco control community
Authors: Chu Kar-Hai , Valente Thomas W 
Published: May 2015

ObjectiveThe sudden growth of e-cigarettes over the last decade has forced advocates and critics scrambling to bolster support for their respective sides. Bridging the divide in geographic barriers, social networking sites were an ideal meeting place for international activist communities, affording them the ability to organise events and discuss new topics in real time. This study examines how e-cigarettes are addressed in GLOBALink, an online international tobacco control community. We seek to discover if the pattern of activity in e-cigarette discussions changes over time. We are also interested in understanding the characteristics of sentiment toward e-cigarettes in discussion topics between countries with different network characteristics.DesignNetwork analysis to explore the relationships between members from different countries, and sentiment analysis of messages and threads to identify patterns of how different countries address e-cigarette topics.SettingGLOBALink, an online international tobacco control community.ParticipantsNetwork analysis based on GLOBALink members from 37 different countries. Sentiment analysis based on 853 posted messages, with over 1.4 million words.Outcome measuresNetwork centrality measures in country interaction data, including degree, closeness and betweenness. Sentiment scores for each message, and differences between country scores.ResultsThe network analysis found a core/periphery structure where central countries focused on active positive discussions pertaining to e-cigarettes, while isolated and peripheral countries posted negative topics without many responses. A qualitative examination of message topics suggests that general subjects elicit more interactions than those that are context specific.ConclusionsE-cigarettes are a polarising topic that can be seen in how countries appear to discuss related topics with others who share the same opinions. More work is needed to help communities stay informed of current research, and diffuse objective information. Network and sentiment analyses offer a strong combination of methodologies that can help support such efforts.
Prognostic impact of concordant and discordant bone marrow involvement and cell-of-origin in Korean patients with diffuse large B-cell lymphoma treated with R-CHOP
Authors: Park Mi-Jung , Park Soon-Ho , Park Pil-Whan , Seo Yiel-Hea , Kim Kyung-Hee , Seo Ja-Young , Jeong Ji-Hun , Kim Moon Jin , Ahn Jeong-Yeal , Hong Junshik 
Published: May 2015

AimsPrevious studies have suggested many prognostic factors in diffuse large B-cell lymphoma (DLBCL), but the prognostic importance of cell-of-origin and discordant bone marrow involvement remains unclear. The aim of this study was to evaluate the prognostic impact of bone marrow involvement histological subtype, cell-of-origin subtype and international prognostic index (IPI) scores in patients with DLBCL.MethodsPatients who were newly diagnosed with DLBCL and treated with rituximab plus cyclophosphamide, doxorubicin, vincristine and prednisone (R-CHOP) were analysed. Clinical information was reviewed retrospectively. Patients were classified into negative, concordant and discordant bone marrow involvement by histological review. The cell-of-origin types were defined using immunohistochemical analysis.ResultsBoth concordant and discordant bone marrow involvement had a negative prognostic impact on progression-free survival, independent of the standard and National Comprehensive Cancer Network (NCCN) IPI scores and cell-of-origin. Patients with non-germinal centre B-cell type showed significantly shorter progression-free survival than those with germinal centre B-cell type. However, non-germinal centre B-cell type did not have a prognostic impact on progression-free survival or overall survival after controlling for the standard and NCCN-IPI and bone marrow involvement.ConclusionsBoth concordant and discordant bone marrow involvement had an adverse prognostic impact on progression-free survival and overall survival; this was independent of the standard and NCCN-IPI and cell-of-origin (non-germinal centre B-cell type). The NCCN-IPI had more powerful prognostic value than the standard IPI (sIPI). The non-germinal centre B-cell type lost significant prognostic impact on progression-free survival after adjustment for standard and NCCN-IPI and bone marrow involvement.