bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of an electronic wellness instrument in the integrated health and social care of older adults: a group concept mapping study
Authors: Northwood Melissa , Saari Margaret , Heckman George , Alexander Ted , Eastway Bill , Gerantonis Patricia , Gillies Deanne , Gregg Susie , McKinnon Wilson Jane , Morrison Adam , Sultan Heebah , Turcotte Luke 
Published: July 2024

Abstract
Background
Health system fragmentation directly contributes to poor health and social outcomes for older adults with multiple chronic conditions and their care partners. Older adults often require support from primary care, multiple specialists, home care, community support services, and other health-care sectors and communication between these providers is unstructured and not standardized. Integrated and interprofessional team-based models of care are a recommended strategy to improve health service delivery to older adults with complex needs. Standardized assessment instruments deployed on digital platforms are considered a necessary component of integrated care. The aim of this study was to develop strategies to leverage an electronic wellness instrument, interRAI Check Up Self Report, to support integrated health and social care for older adults and their care partners in a community in Southern Ontario, Canada.

Methods
Group concept mapping, a participatory mixed-methods approach, was conducted. Participants included older adults, care partners, and representatives from: home care, community support services, specialized geriatric services, primary care, and health informatics. In a series of virtual meetings, participants generated ideas to implement the interRAI Check Up and rated the relative importance of these ideas. Hierarchical cluster analysis was used to map the ideas into clusters of similar statements. Participants reviewed the map to co-create an action plan.

Results
Forty-one participants contributed to a cluster map of ten action areas (e.g., engagement of older adults and care partners, instrument’s ease of use, accessibility of the assessment process, person-centred process, training and education for providers, provider coordination, health information integration, health system decision support and quality improvement, and privacy and confidentiality). The health system decision support cluster was rated as the lowest relative importance and the health information integration was cluster rated as the highest relative importance.

Conclusions
Many person-, provider-, and system-level factors need to be considered when implementing and using an electronic wellness instrument across health- and social-care providers. These factors are highly relevant to the integration of other standardized instruments into interprofessional team care to ensure a compassionate care approach as technology is introduced.
Metabolic reprogramming and heterogeneity during the decidualization process of endometrial stromal cells
Authors: Jia Zhaoyu , Wei Yuan , Zhang Ye , Song Kun , Yuan Jia 
Published: July 2024

AbstractThe human endometrial decidualization is a transformative event in the pregnant uterus that involves the differentiation of stromal cells into decidual cells. While crucial to the establishment of a successful pregnancy, the metabolic characteristics of decidual cells in vivo remain largely unexplored. Here, we integrated the single-cell RNA sequencing (scRNA-seq) datasets on the endometrium of the menstrual cycle and the maternal-fetal interface in the first trimester to comprehensively decrypt the metabolic characteristics of stromal fibroblast cells. Our results revealed that the differentiation of stromal cells into decidual cells is accompanied by increased amino acid and sphingolipid metabolism. Furthermore, metabolic heterogeneity exists in decidual cells with differentiation maturity disparities. Decidual cells with high metabolism exhibit higher cellular activity and show a strong propensity for signaling. In addition, significant metabolic reprogramming in amino acids and lipids also occurs during the transition from non-pregnancy to pregnancy in the uteri of pigs, cattle, and mice. Our analysis provides comprehensive insights into the dynamic landscape of stromal fibroblast cell metabolism, contributing to our understanding of the metabolism at the molecular dynamics underlying the decidualization process in the human endometrium.
CircRHBDD1 promotes immune escape via IGF2BP2/PD-L1 signaling and acts as a nanotherapeutic target in gastric cancer
Authors: Li Yanna , Wang Zhixiong , Gao Peng , Cao Danping , Dong Runyu , Zhu Menglin , Fei Yao , Zuo Xueliang , Cai Juan 
Published: July 2024

Abstract
Background
Circular RNAs (circRNAs) have been implicated in the development and progression of gastric cancer (GC). However, it remains unclear whether dysregulated circRNA affects immune escape and the efficacy of immunotherapy in GC. Our aim is to investigate the molecular mechanism of circRNA affecting GC immunotherapy and identify effective molecular therapeutic targets.

Methods
The differential expression profile of circRNAs was established through circRNA sequencing, comparing three paired GC tissues with their adjacent non-cancerous gastric tissues. The expression level of circRHBDD1 in GC tissues was then assessed using quantitative reverse transcription polymerase chain reaction (qRT-PCR). The biological characteristics of circRHBDD1 were verified through a series of experiments, including agarose gel electrophoresis assays, RNase R treatment, and actinomycin D experiments. The prognostic value of circRHBDD1 in GC was evaluated by conducting both univariate and multivariate survival analyses. Furthermore, loss- and gain-of-function approaches were utilized to investigate the impact of circRHBDD1 on GC immune escape. RNA-sequencing, immunoprecipitation, flow cytometry, and methylated RNA immunoprecipitation (meRIP) analysis were performed to elucidate the underlying molecular mechanisms.

Results
We discovered that circRHBDD1 exhibited remarkably high expression levels in GC tissues and cell lines. Notably, the high expression of circRHBDD1 was significantly correlated with poor overall survival and disease-free survival among GC patients. Both in vitro and in vivo experiments revealed that circRHBDD1 upregulated the expression of PD-L1 and impeded the infiltration of CD8+ T cells. Further, we found that circRHBDD1 binds to IGF2BP2, disrupting the interaction between E3 ligase TRIM25 and IGF2BP2, and ultimately inhibiting IGF2BP2 ubiquitination and degradation. Intriguingly, IGF2BP2 enhances PD-L1 mRNA stability through m6A modification. Additionally, we developed Poly (lactide-co-glycolic acid) (PLGA)-Polyethylene glycol (PEG)-based nanoparticles loaded with circRHBDD1 siRNA. In vivo experiments validated that the combination of PLGA-PEG(si-circRHBDD1) and anti-PD-1 offers a safe and efficacious nano-drug regimen for cancer immunotherapy.

Conclusion
Our results demonstrated that circRHBDD1 promoted GC immune escape by upregulating the expression of PD-L1 and reprogramming T cell-mediated immune response. Inhibition of circRHBDD1 expression could potentially enhance the response of GC patients to immunotherapy, thus improving treatment outcomes. Additionally, the development of a nanodrug delivery system provides a feasible approach for future clinical applications.
Association between dietary branched-chain amino acids and multiple chronic conditions among older adults in Chinese communities
Authors: Song Yuanfeng , Zhang Ji , Luo Ziqiang , Wu Lanlan , Cai Zhaopei , Zhong Xiaoqi , Zeng Xiaoxue , Cao Tingxi , Chen Hong-en , Xu Shan , Wang Chang-yi 
Published: July 2024

Abstract
Background
The association of BCAAs (isoleucine, leucine, and valine) with cardiovascular and cerebrovascular diseases has been widely recognized by researchers, but there is limited evidence to support the relationship between BCAAs and multiple chronic conditions (MCCs) in older adults. This study aimed to explore the correlation between BCAA levels in the diets of older adults and MCCs.

Methods
Based on a health management cohort project in Nanshan District of Shenzhen, 4278 individuals over 65 years old were selected as participants via multi-stage stratified sampling from May 2018 to December 2019. Data were collected using a validated semi-quantitative food frequency questionnaire, as well as anthropometric and chronic disease reports. MCC was defined as the coexistence of two or more chronic diseases, namely, hypertension, dyslipidemia, diabetes, CAD, stroke, CKD, and CLD. Multivariate unconditional logistic regression analysis was used to analyze the relationship between dietary BCAAs and MCCs in older adults, and then, gender stratification analysis was performed. A restricted cubic spline model (a fitted smooth curve) was used to determine the dose–response relationship of isoleucine with MCCs.

Results
A total of 4278 older adults aged 65 and above were included in this study, with an average age of 72.73 ± 5.49 years. The cohort included 1861 males (43.50%). Regardless of whether confounding factors were corrected, isoleucine was a risk factor for MCCs (OR = 3.388, 95%CI:1.415,8.109). After gender stratification, the relationships between dietary isoleucine and MCCs (OR = 6.902, 95%CI:1.875,25.402) and between leucine (OR = 0.506,95%CI:0.309,0.830) and MCCs were significant in women, but not in men. No significant association between valine and MCCs was observed. In addition, isoleucine was a risk factor for MCCs when its intake was greater than 4.297 g/d.

Conclusion
Isoleucine may play an important role in regulating age-related diseases. BCAAs such as isoleucine can be used as risk markers for MCCs in older adults.
Impact of screw tip design on screw anchorage: mechanical testing and numerical simulation
Authors: Xie Shiming , Cui Liqiang , Liu Jinhui , Qing Peidong , Li Jingchi 
Published: July 2024

Abstract
Background
Screw loosening is a commonly reported issue following spinal screw fixation and can lead to various complications. The initial cause of screw loosening is biomechanical deterioration. Previous studies have demonstrated that modifications in screw design can impact the local biomechanical environment, specifically the stress distribution on bone-screw interfaces. There are several different designs of screw tips available for clinically used pedicle screws; however, it remains unclear whether these variations affect the local stress distribution and subsequent screw anchorage ability.

Methods
This study conducted comprehensive biomechanical research using polyurethane foam mechanical tests and corresponding numerical simulations to investigate this topic. Models of pedicle screw-fixed osteoporotic polyurethane foam were created with two different clinically used screw tip designs (flat and steep) featuring varying tip lengths, taper angles, and diameters, as well as identical flank overlap areas and thread designs. The anchorage ability of the different models was assessed through toggle and pull-out test. Additionally, numerical mechanical models were utilized to compute the stress distributions at the screw and bone-screw interfaces in the different models.

Results
Mechanical tests revealed superior anchorage ability in models utilizing flat-tipped screws. Furthermore, numerical modeling indicated improved anchorage ability and reduced stress concentration tendency in these models.

Conclusion
Changes in screw tip design can significantly impact the biomechanical anchoring capability of screws. Specifically, flatter tip pedicle screws may mitigate the risk of screw loosening by alleviating stress concentration on bone-screw interfaces.
Health economic evaluation of an electronic mindfulness-based intervention (eMBI) to improve maternal mental health during pregnancy – a randomized controlled trial (RCT)
Authors: Hasemann Lena , Elkenkamp Svenja , Müller Mitho , Bauer Armin , Wallwiener Stephanie , Greiner Wolfgang 
Published: July 2024

Abstract
Background
Anxiety and depression are the most prevalent psychiatric diseases in the peripartum period. They can lead to relevant health consequences for mother and child as well as increased health care resource utilization (HCRU) and related costs. Due to the promising results of mindfulness-based interventions (MBI) and digital health applications in mental health, an electronic MBI on maternal mental health during pregnancy was implemented and assessed in terms of transferability to standard care in Germany. The present study focused the health economic outcomes of the randomized controlled trial (RCT).

Methods
The analysis, adopting a payer’s and a societal perspective, included women of increased emotional distress at < 29 weeks of gestation. We applied inferential statistics (α = 0.05 significance level) to compare the intervention group (IG) and control group (CG) in terms of HCRU and costs. The analysis was primarily based on statutory health insurance claims data which covered the individual observational period of 40 weeks.

Results
Overall, 258 women (IG: 117, CG: 141) were included in the health economic analysis. The results on total health care costs from a payer’s perspective indicated higher costs for the IGi compared to the CG (Exp(ß) = 1.096, 95% CI: 1.006–1.194, p = 0.037). However, the estimation was not significant after Bonferroni correction (p < 0.006). Even the analysis from a societal perspective as well as sensitivity analyses did not show significant results.

Conclusions
In the present study, the eMBI did neither reduced nor significantly increased health care costs. Further research is needed to generate robust evidence on eMBIs for women suffering from peripartum depression and anxiety.

Trial registration
German Clinical Trials Register: DRKS00017210. Registered on 13 January 2020. Retrospectively registered.
Existence of nontrivial solutions for a fractional $p\&q$-Laplacian equation with sandwich-type and sign-changing nonlinearities
Authors: Li Qin , Xiu Zonghu , Chen Lin 
Published: July 2024

AbstractIn this paper, we deal with the following fractional $p\&q$
p
&
q
-Laplacian problem: $$ \left \{ \textstyle\begin{array}{l@{\quad }l} (-\Delta )_{p}^{s}u +(-\Delta )_{q}^{s}u =\lambda a(x)|u|^{\theta -2}u+ \mu b(x)|u|^{r-2}u&\text{in}\;\ \Omega , \\ u(x)=0 &\text{in}\;\ \mathbb{R}^{N}\setminus \Omega , \end{array}\displaystyle \right . $$

{

(
−
Δ
)

p
s

u
+

(
−
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)

q
s

u
=
λ
a
(
x
)

|
u
|

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−
2

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+
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(
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)

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u
|

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−
2

u

in

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,

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(
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=
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in

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∖
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,

where $\Omega \subset \mathbb{R}^{N}$
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is a bounded domain with smooth boundary, $s\in (0,1)$
s
∈
(
0
,
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, $(-\Delta )_{m}^{s}$

(
−
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)

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s

$(m\in \{p,q\})$
(
m
∈
{
p
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is the fractional m-Laplacian operator, $p,q,r,\theta \in (1,p_{s}^{*}]$
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,
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∈
(
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,

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, $p_{s}^{*}=\frac{Np}{N-sp}$

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, $\lambda , \mu >0$
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,
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>
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, and the weights $a(x)$
a
(
x
)
and $b(x)$
b
(
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)
are possibly sign changing. Using the concentration compactness principle for fractional Sobolev spaces and the Ekeland variational principle, we prove that the problem admits a nonnegative solution for the critical case $r=p_{s}^{*}$
r
=

p
s
∗

. Moreover, for the subcritical case $r< p_{s}^{*}$
r
<

p
s
∗

, we obtain two existence results by applying the Ekeland variational principle and the mountain pass theorem.
Perceived comfort with weight, body shape and eating pattern of young adults with type 1 diabetes and associations with clinical and psychological parameters in a clinical setting
Authors: Vidyasagar Sneha , Griffin Alison , d’Emden Helen , Hendrieckx Christel , D’Silva Neisha 
Published: July 2024

Abstract
Background
Higher prevalence of disordered eating in young adults with type 1 diabetes (T1D) culminates in higher levels of morbidity and mortality. In addition to validated questionnaires for diabetes distress, depression/anxiety symptoms and emotional well-being, the Diabetes Psychosocial Assessment Tool (DPAT) includes three questions about comfort with weight, body shape and eating pattern (WSE), which were derived from literature and multidisciplinary team consensus. Recognising individuals with low comfort with WSE, is the first step towards identifying those who may be at risk of developing eating disorders.

Aims
Observe comfort with WSE, in young adults with T1D, and its associations with demographic/clinical characteristics and psychological parameters.

Methods
276 young adults, aged 15–26, who attended routine clinical care at a Young Adult Diabetes Clinic, completed the DPAT. The WSE questions were scored on a 5-point Likert scale (1 indicating lowest comfort). Linear regression analysed differences in comfort with weight and eating pattern by demographic and psychological parameters.

Results
1 in 3 young adults (29%) reported low comfort with WSE (scores 1 or 2). In females, 40%, 41% and 35% had low comfort with weight, shape and eating patterns respectively, in comparison to males in whom it was 18.5%, 16% and 21.5%. Females reported lower comfort with weight and eating pattern (mean 2.9 and 3.0 respectively) than Males (mean 3.7 and 3.6 respectively), each p < 0.001. Lower comfort with weight (p < 0.001) and eating pattern (p = 0.001) was associated with higher body mass index (BMI). Young adults with low comfort with weight and eating pattern experienced elevated diabetes distress and depressive/anxiety symptoms (each p < 0.001), also when adjusted for sex and BMI.

Conclusions
The study has shown that low comfort with WSE is common among young adults with T1D. Adding these questions into routine care, can allow for easy and early identification of low comfort, initiation of a therapeutic dialogue and implementation of focused management strategies.
The Brief Symptom Inventory-9 (BSI-9): Development and validation in a German general population sample
Authors: MacDonald C. , Brophy K. , Coroiu A. , Braehler Elmar , Körner A. 
Published: July 2024

Abstract
Background
The Brief Symptom Inventory-18 (BSI-18) is a self-report questionnaire with three subscales, somatisation, anxiety, and depression, based on longer measures of distress. The present study proposes a shorter, nine-item version (BSI-9) of the BSI-18 as a brief screening tool for distress.

Methods
Confirmatory factor analyses and reliability and validity analyses were carried out using a representative sample of the German general population. Confirmatory factor analysis demonstrates a good model fit for the three-dimensional BSI-9.

Results
The total scale was found to have strong internal consistency (αCronbach = 0.87 for the global severity index). The internal consistency coefficients of the three-item subscales reflect the brevity of these scales (somatisation ﻿αCronbach = 0.72, depression ﻿α Cronbach = 0.79, anxiety ﻿αCronbach = 0.68). The subscales were found to be significantly related with subscales of the Patient Health Questionnaire-4 and Hopkins Symptom Checklist-25.

Limitations
The present study used a limited number of distress measures, and a more recent dataset would be useful to provide a more current picture of the general population’s distress levels.

Conclusions
The BSI-9 provides a short, valid, and reliable screener for distress in the general population. Future work should examine its utility in clinical settings and different cultural contexts.
Lysine methylation modifications in tumor immunomodulation and immunotherapy: regulatory mechanisms and perspectives
Authors: Luo Yiming , Lu Junli , Lei Zhen , Zhu He , Rao Dean , Wang Tiantian , Fu Chenan , Zhang Zhiwei , Xia Limin , Huang Wenjie 
Published: July 2024

AbstractLysine methylation is a crucial post-translational modification (PTM) that significantly impacts gene expression regulation. This modification not only influences cancer development directly but also has significant implications for the immune system. Lysine methylation modulates immune cell functions and shapes the anti-tumor immune response, highlighting its dual role in both tumor progression and immune regulation. In this review, we provide a comprehensive overview of the intrinsic role of lysine methylation in the activation and function of immune cells, detailing how these modifications affect cellular processes and signaling pathways. We delve into the mechanisms by which lysine methylation contributes to tumor immune evasion, allowing cancer cells to escape immune surveillance and thrive. Furthermore, we discuss the therapeutic potential of targeting lysine methylation in cancer immunotherapy. Emerging strategies, such as immune checkpoint inhibitors (ICIs) and chimeric antigen receptor T-cell (CAR-T) therapy, are being explored for their efficacy in modulating lysine methylation to enhance anti-tumor immune responses. By targeting these modifications, we can potentially improve the effectiveness of existing treatments and develop novel therapeutic approaches to combat cancer more effectively.
Ancient paintings inpainting based on dual encoders and contextual information
Authors: Sun Zengguo , Lei Yanyan , Wu Xiaojun 
Published: July 2024

Abstract
Deep learning-based inpainting models have achieved success in restoring natural images, yet their application to ancient paintings encounters challenges due to the loss of texture, lines, and color. To address these issues, we introduce an ancient painting inpainting model based on dual encoders and contextual information to overcome the lack of feature extraction and detail texture recovery when restoring ancient paintings. Specifically, the proposed model employs a gated encoding branch that aims to minimize information loss and effectively capture semantic information from ancient paintings. A dense multi-scale feature fusion module is designed to extract texture and detail information at various scales, while dilated depthwise separable convolutions are utilized to reduce parameters and enhance computational efficiency. Furthermore, a contextual feature aggregation module is incorporated to extract contextual features, enhancing the overall consistency of the inpainting results. Finally, a color loss function is introduced to ensure color consistency in the restored area, harmonizing it with the surrounding region. The experimental results indicate that the proposed model effectively restores the texture details of ancient paintings, outperforming other methods both qualitatively and quantitatively. Additionally, the model is tested on real damaged ancient paintings to validate its practicality and efficacy.
High-resolution live cell imaging to define ultrastructural and dynamic features of the halotolerant yeast Debaryomyces hansenii
Authors: Xelhuantzi Martha S. C. , Ghete Daniel , Milburn Amy , Ioannou Savvas , Mudd Phoebe , Calder Grant , Ramos José , O'Toole Peter J. , Genever Paul G. , MacDonald Chris 
Published: July 2024

ABSTRACT
Although some budding yeasts have proved tractable and intensely studied models, others are more recalcitrant. Debaryomyces hansenii, an important yeast species in food and biotechnological industries with curious physiological characteristics, has proved difficult to manipulate genetically and remains poorly defined. To remedy this, we have combined live cell fluorescent dyes with high-resolution imaging techniques to define the sub-cellular features of D. hansenii, such as the mitochondria, nuclei, vacuoles and the cell wall. Using these tools, we define biological processes like the cell cycle, organelle inheritance and various membrane trafficking pathways of D. hansenii for the first time. Beyond this, reagents designed to study Saccharomyces cerevisiae proteins were used to access proteomic information about D. hansenii. Finally, we optimised the use of label-free holotomography to image yeast, defining the physical parameters and visualising sub-cellular features like membranes and vacuoles. Not only does this work shed light on D. hansenii but this combinatorial approach serves as a template for how other cell biological systems, which are not amenable to standard genetic procedures, can be studied.