bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Location of Pretreatment Hyperdense Middle Cerebral Artery Sign Predicts the Outcome of Intraarterial Thrombectomy for Acute Stroke
Authors: Man Shumei , Hussain Muhammad Shazam , Wisco Dolora , Katzan Irene L. , Aoki Junya , Tateishi Yohei , Cheng‐Ching Esteban , Hui Ferdinand K. , Masaryk Thomas J. , Rasmussen Peter A. , Uchino Ken 
Published: April 2014

ABSTRACTBACKGROUND AND PURPOSEIntraarterial (IA) mechanical thrombectomy has an excellent recanalization rate but does not always correlate with good clinical outcomes. We aimed to investigate whether hyperdense middle cerebral artery sign (HMCAS) on preintervention nonenhanced CT (NECT) predicts IA therapy outcome for acute stroke.METHODSData were abstracted from our Hyperacute Ischemic Stroke database. Patients with occlusion in ICA, MCA, or MCA M2 branches who underwent IA therapy were included.RESULTSAmong 126 patients who underwent IA treatment, 64 (51%) had hyperdense M1 MCA sign (M1 HMCAS), 11 (9%) had hyperdense M2, and 51 (40%) had No HMCAS (NHMCAS).M1 HMCAS and NHMCAS group has comparable baseline stroke severity and infarct volume (P > .05); and the differences of favorable outcome (modified Rankin Score 0‐2) at 30 days were not significant (21% vs. 30%, P = .259). For those with HMCAS, favorable 30‐day outcome was most frequent in Distal HMCAS (39%), followed by hyperdense M2 (27%), HMCAS proximal (11%), and HMCAS full length (0%).CONCLUSIONSFor acute ischemic stroke due to large vessel occlusion, the lack of HMCAS on NECT does not predict favorable outcome after IA therapy. Among those with HMCAS, proximal and longer HMCAS predicts unfavorable outcome.
Computed tomographic appearance of canine thyroid tumours
Authors: Deitz K. , Gilmour L. , Wilke V. , Riedesel E. 
Published: April 2014

OBJECTIVESTo describe the computed tomography features of canine thyroid tumours.METHODS Retrospective study of records of dogs with a thyroid tumour and neck computed tomography. Neck computed tomographies were evaluated for tumour characteristics. Thoracic radiographs and computed tomographies were evaluated for lung nodules.RESULTSOf 19 identified cases, 17 were carcinomas and 2 were adenomas; 12 had mineralisation, 16 had heterogeneous attenuation and 16 were unilateral. Tumours were located from the temporomandibular joint toC5. Sixteen had well‐defined margins postcontrast. Tumours were ovoid and mean volume was 57·4 cm3. By computed tomography, eight had definitive or possible invasion into surrounding structures; all eight were histopathologically invasive carcinomas. Five histopathologically non‐invasive tumours and two adenomas had no computed tomography invasion into surrounding structures. Four had complete palpable mobility (two adenomas and two histopathologically invasive carcinomas); one had computed tomography evidence of possible invasion. The sensitivity of palpable mass mobility to determine histopathological invasion was 71% with 0% specificity. The sensitivity of computed tomography invasion to determine histopathological invasion was 70% with 100% specificity.CLINICAL SIGNIFICANCEComputed tomography scans revealed several common features. Palpable mass mobility was not definitive for lack of histopathological invasion. Computed tomography invasion was specific but not very sensitive for histopathological invasion.
Assessment of a Phytogenic Feed Additive (Digestarom P.E.P. MGE) on Growth Performance, Processing Yield, Fillet Composition, and Survival of Channel Catfish
Authors: Peterson Brian C. , Bosworth Brian G. , Li Menghe H. , Beltran Ruben , Santos Goncalo A. 
Published: April 2014

AbstractWe investigated the effects of a phytogenic feed additive (Digestarom® P.E.P. MGE) on growth performance, processing yield, fillet composition, and survival of pond‐raised channel catfish. Fifteen 0.4 ha ponds were stocked with 14,820 catfish (126 g/fish) per ha. Fish in control ponds were fed a 32% crude protein commercial floating diet whereas fish in test ponds were fed the same diet supplemented with Digestarom P.E.P. MGE at 200 g/ton. In a second study, ten 0.04 ha ponds were stocked at a similar density with fish that averaged 68 g/fish. At the end of the 6‐mo study, there was no significant difference in the amount of feed fed or the amount of weight gained between the control and Digestarom P.E.P. MGE fed fish. Food conversion ratio, net yield, and survival were also similar between the two groups. Carcass, fillet, and nugget yield were similar. Fillet proximate analysis revealed that fillet fat was lower (P < 0.01) whereas fillet protein tended to be a little higher (P < 0.10) in treated fish. In conclusion, there was a significant reduction in the amount of fillet fat in Digestarom P.E.P. MGE fed fish. Improved fillet composition (higher protein and lower lipid) is of commercial importance.
Immune Responses to Vibrio anguillarum in Yellowtail Kingfish, Seriola lalandi, Fed Selenium Supplementation
Authors: Le Ky T. , Fotedar Ravi 
Published: April 2014

AbstractEffects of dietary selenium (Se) on immune competence of yellowtail kingfish, Seriola lalandi, were investigated. The fish were fed one of the three experimental diets including a control diet without Se supplementation and two diets supplemented with Se from Se‐yeast (Selplex) at 2 and 4 mg/kg. After feeding for 6 wk, the fish were challenged by injecting Vibrio anguillarum and observed for 2 wk. Dietary Se had no effect on feed intake, feed conversion ratio, and survival over the course of 6‐wk feeding; however, it significantly increased weight gain and Se content in muscle. Following the bacterial infection, the immune‐stimulating effects of Se were observed in antibody, lysozyme, and bactericidal responses, and there was a corresponding increase in survival and hematocrit by Se. Under infectious condition, antioxidant capacity of fish as measured in terms of resistance of red blood cells to peroxidation and glutathione peroxidase activity also increased by supplementation of Se. Liver necrosis and kidney melano‐macrophages were only seen in surviving fish fed the control diet after the challenge. Furthermore, there was evidence of myopathy in fish fed the diet without Se supplementation. This study suggests that Se, supplemented at 2 or 4 mg/kg, can improve growth and health of yellowtail kingfish.
Practical Considerations of Molecular Parentage Analysis in Fish
Authors: Yue Gen Hua , Xia Jun Hong 
Published: April 2014

AbstractPedigree information is important in selective breeding programs and hatchery management of aquaculture. However, in aquaculture species, tagging of young fish with physical tags is very difficult. Molecular parentage analyses have been used in reconstructing pedigrees in aquaculture species since the 1990s. Some studies have been carried out to optimize every step to reduce the cost and improve the efficiency of molecular parentage analysis. However, molecular parentage analysis of a large number of individuals is still too expensive because some steps of molecular parentage analysis are very cost‐intensive. In this review, we have summarized the techniques of major steps in parentage analysis, and provided a guide for aquaculture scientists on how to cost‐effectively and rapidly extractDNAfrom a large number of samples, select the right types ofDNAmarkers, optimize multiplex polymerase chain reaction, and select appropriate methods and software to analyze the genotyping data for reconstructing pedigrees. In addition, we discuss the challenges and future directions of molecular parentage analysis in aquaculture species.
The Effects of Soybean Lecithin Supplementation to a Practical Diet Formulation on Juvenile Channel Catfish, Ictalurus punctatus: Growth, Survival, Hematology, Innate Immune Activity, and Lipid Biochemistry
Authors: Sink Todd D. , Lochmann Rebecca T. 
Published: April 2014

AbstractA feeding trial was conducted to determine the effects of soy lecithin supplementation on production performance of juvenile channel catfish, Ictalurus punctatus (mean ± SE; 5.8 ± 0 g). The basal diet consisted of a practical dietary formulation for channel catfish, containing 4.3% endogenous phospholipids (PL) from dietary ingredients, to which supplemental PL from soybean lecithin were added. The study diets were 1 control and 2 experimental diets to which 0, 2, or 4% supplemental lecithin was added, respectively. Soy lecithin inclusion did not affect survival, growth, feed consumption, whole‐body total lipid, innate immune response, plasma cholesterol or triglyceride concentrations, or hepatosomatic index. Feed conversion (gain/intake) improved in fish fed 4% supplemental lecithin compared with 0% lecithin. Whole‐body crude protein was greater in fish fed 2% supplemental lecithin compared with 0% lecithin, while 4% supplemental lecithin was intermediate. Phosphatidylcholine (PC) content was greater in fish fed 2 or 4% lecithin than 0% lecithin. Plasma concentrations of PC were inversely proportional to dietary concentrations. Liver glycogen was greater in fish fed 0% lecithin compared with 2 or 4% lecithin. Liver lipid and phospholipid were lower in fish fed 0% lecithin than 2 or 4% lecithin. The dietary phospholipid requirement, if any, of juvenile channel catfish for growth and survival is less than or equal to 4.3% (1.5% PC) of the diet. Feed conversion is improved in channel catfish fed diets supplemented with 4% soy lecithin (7.2% phospholipid; 5.1% PC), which might offset additional costs due to phospholipid supplementation. Dietary soy lecithin inclusion altered plasma and liver lipid composition, but it is unknown whether these effects can alter the ability of juvenile catfish to survive and grow under various conditions.
Examination of the Effects of Dietary Protein and Lipid Levels on Growth and Stress Tolerance of Juvenile Tilapia, Oreochromis niloticus
Authors: Hooley Christopher G. , Barrows Frederic T. , Paterson John , Sealey Wendy M. 
Published: April 2014

AbstractTilapia is the second most consumed farmed fish after carp and the most widely grown farmed fish. However, significant price increases in ingredients in recent years threaten profitability of the industry because dietary costs are approximately 60% of production costs. Therefore, the objective of this study was to optimize dietary protein and lipid levels of juvenile tilapia cultured in high‐intensity recirculating‐water system and assess dietary effects on stress tolerance. To achieve this objective, a factorial treatment design with diets formulated to contain three levels of dietary protein (28, 32, and 36%) and three levels of dietary lipid (3, 6, and 9%) was employed. Juvenile tilapia (34.5 ± 0.4 g initial weight) were randomly assigned to each of 24 tanks and fed one of the nine diets, three feedings per day to apparent satiation, 6 d/wk for 12 wk. Fish were weighed and counted every 3 wk and feed consumed recorded weekly. Tilapia weight gain, feed conversion, feed intake, and proximate composition were significantly affected by diet. Following the feeding trial, tilapia were subjected to a 24 h simulated live haul where fish were stocked into a static water system at 0.24 kg/L of water. Glucose, lactate, and cortisol measurements were taken at time 0, 24, and 72 h after fish were placed into hauling containers. There was significant effects (P < 0.05) of crude lipid and crude protein on blood chemistry parameters. These data indicate that practical diet formulations can be improved to enhance growth rates, production of tilapia, and stress response during live hauls.
The Efficacy of Copper Sulfate in Controlling Infection of Saprolegnia parasitica
Authors: Sun Qi , Hu Kun , Yang Xian‐Le 
Published: April 2014

AbstractSaprolegnia parasitica is a severe fish pathogen that causes important economic losses worldwide. Copper is an important additive in the aquaculture industry for control of algal growth, ectoparasites, and fungal disease. However, at present no data is available on the specific interaction of copper sulfate with oomycete. In our study, the efficacy of copper sulfate on the mycelium and zoospore of S. parasitica was assessed in vitro, and S. parasitica infection experiment was conducted to assess its performance in vivo. The results indicated that copper sulfate at ≥0.5 mg/L inhibited the growth of mycelium, no primary zoospores were released at ≥1.0 mg/L. Additionally, 0.5 mg/L copper sulfate could reduce the infection rate of S. parasitica in the grass carp, Ctenopharyngodon idellus. This study demonstrates the good efficacy of copper sulfate on the control of S. parasitica infection in grass carps, and suggests that copper sulfate could be used as a drug additive to control the S. parasitica infection in the aquaculture industry.
Exploring Picea glauca aquaporins in the context of needle water uptake and xylem refilling
Authors: Laur Joan , Hacke Uwe G. 
Published: April 2014

Summary

Conifer needles have been reported to absorb water under certain conditions. Radial water movement across needle tissues is likely influenced by aquaporin (AQP) water channels.

Foliar water uptake and AQP localization in Picea glauca needles were studied using physiological and microscopic methods. AQP expression was measured using quantitative real‐time PCR. Members of the AQP gene family in spruce were identified using homology search tools.

Needles of drought‐stressed plants absorbed water when exposed to high relative humidity (RH). AQPs were present in the endodermis‐like bundle sheath, in phloem cells and in the transfusion parenchyma of needles. Up‐regulation of AQPs in high RH coincided with embolism repair in stem xylem. The present study also provides the most comprehensive functional and phylogenetic analysis of spruce AQPs to date. Thirty putative complete AQP sequences were found.

Our findings are consistent with the hypothesis that AQPs facilitate radial water movement from the needle epidermis towards the vascular tissue. Foliar water uptake may occur in late winter when needles are covered by melting snow and may provide a water source for embolism repair before the beginning of the growing season.
High‐throughput fluorescence‐activated cell sorting for lipid hyperaccumulating Chlamydomonas reinhardtii mutants
Authors: Xie Bo , Stessman Dan , Hart Jason H. , Dong Haili , Wang Yingjun , Wright David A. , Nikolau Basil J. , Spalding Martin H. , Halverson Larry J. 
Published: April 2014

SummaryThe genetically tractable microalga Chlamydomonas reinhardtii has many advantages as a model for renewable bioproducts and/or biofuels production. However, one limitation of C. reinhardtii is its relatively low‐lipid content compared with some other algal species. To overcome this limitation, we combined ethane methyl sulfonate mutagenesis with fluorescence‐activated cell sorting (FACS) of cells stained with the lipophilic stain Nile Red to isolate lipid hyperaccumulating mutants of C. reinhardtii. By manipulating the FACS gates, we sorted mutagenized cells with extremely high Nile Red fluorescence signals that were rarely detected in nonmutagenized populations. This strategy successfully isolated several putative lipid hyperaccumulating mutants exhibiting 23% to 58% (dry weight basis) higher fatty acid contents than their progenitor strains. Significantly, for most mutants, nitrogen starvation was not required to attain high‐lipid content nor was there a requirement for a deficiency in starch accumulation. Microscopy of Nile Red stained cells revealed that some mutants exhibit an increase in the number of lipid bodies, which correlated with TLC analysis of triacyglycerol content. Increased lipid content could also arise through increased biomass production. Collectively, our findings highlight the ability to enhance intracellular lipid accumulation in algae using random mutagenesis in conjunction with a robust FACS and lipid yield verification regime. Our lipid hyperaccumulating mutants could serve as a genetic resource for stacking additional desirable traits to further increase lipid production and for identifying genes contributing to lipid hyperaccumulation, without lengthy lipid‐induction periods.
Naturalizing Intentionality: Tracking Theories Versus Phenomenal Intentionality Theories
Authors: Mendelovici Angela , Bourget David 
Published: April 2014

AbstractThis paper compares tracking and phenomenal intentionality theories of intentionality with respect to the issue of naturalism. Tracking theories explicitly aim to naturalize intentionality, while phenomenal intentionality theories generally do not. It might seem that considerations of naturalism count in favor of tracking theories. We survey key considerations relevant to this claim, including some motivations for and objections to the two kinds of theories. We conclude by suggesting that naturalistic considerations may in fact support phenomenal intentionality theories over tracking theories.
The Transparency of Mind
Authors: Paul Sarah K. 
Published: April 2014

AbstractIn philosophical inquiry into the mind, the metaphor of ‘transparency’ has been attractive to many who are otherwise in deep disagreement. It has thereby come to have a variety of different and mutually incompatible connotations. The mind is said to be transparent to itself, our perceptual experiences are said to be transparent to the world, and our beliefs are said to be transparent to – a great many different things. The first goal of this essay is to sort out the different uses of the notion of transparency in the context of the philosophy of mind. The remainder of the essay will then be devoted to examining so‐called Transparency theories of self‐knowledge, or how we know our own minds. This type of theory has attracted a great deal of interest in recent years, but its prospects hinge on answers to unresolved questions concerning the epistemological details of the account and the scope of its ambitions.
The Return of the Organism as a Fundamental Explanatory Concept in Biology
Authors: Nicholson Daniel J. 
Published: April 2014

AbstractAlthough it may seem like a truism to assert that biology is the science that studies organisms, during the second half of the twentieth century the organism category disappeared from biological theory. Over the past decade, however, biology has begun to witness the return of the organism as a fundamental explanatory concept. There are three major causes: (a) the realization that the Modern Synthesis does not provide a fully satisfactory understanding of evolution; (b) the growing awareness of the limits of reductionism in molecular biology; and (c) the renewed interest in the nature of life as a genuine scientific problem. This essay examines these recent developments and considers the new epistemological roles being played by the organism in each of them. It also reflects on what the present resurgence of the organism means for the philosophy of biology.
Implicit Knowledge: How it is Understood and Used in Feminist Theory
Authors: Shotwell Alexis 
Published: April 2014

AbstractFeminist theorists have crafted diverse accounts of implicit knowing that exceed the purview of epistemology conventionally understood. I characterize this field as through examining thematic clusters of feminist work on implicit knowledge: phenomenological and foucauldian theories of embodiment; theories of affect and emotion; other forms of implicit knowledge. Within these areas, the umbrella concept of implicit knowledge (or understanding, depending on how it's framed) names either contingently unspoken or fundamentally nonpropositional but epistemically salient content in our experience. I make a case for distinguishing implicit knowledge from lively conversations about ‘implicit bias’, as well as from the ‘know‐how/know‐that’ debates, and I explore key ways the notion of implicit knowledge is currently formulated in feminist philosophy.
Temperature‐specific acclimation effects on adult locomotor performance of inbred and crossbredDrosophila melanogaster
Authors: Kj Ærsgaard Anders , Faurby Søren , Thomsen Hans P. , Loeschcke Volker , Kristensen Torsten N. , Pertoldi Cino 
Published: April 2014

AbstractClimate change poses a serious threat to the existence of many species. The combination of habitat fragmentation and increasing temperatures is of particular concern because it can alter demographic and population genetic processes, which may ultimately lead to extinction. Locomotion is very important in mitigating the negative impacts of these processes by upholding migration and contributing to random mating within and between populations. In the present study, aT‐maze, constituting a relatively complex laboratory assay, is used to investigate whether inbreeding affects the capacity to reach a food source in maleDrosophila melanogasterMeigen 1830 (Diptera:Drosophilidae) reared at 20, 25 or 30 °C, respectively. The effects of inbreeding and crossbreeding are highly temperature‐specific. Strong heterosis for the ability to reach food in the maze is observed in flies developed and maintained at 30 °C, whereas inbred flies locate the food significantly faster than crossbreds when reared at 25 °C in four of six runs. No clear pattern is evident in flies reared at 20 °C. The results suggest that complex traits such as locomotor performance in a maze are highly informative in the evaluation and detection of inbreeding depression under different thermal conditions. The effect of inbreeding is most pronounced at high temperature, which is a characteristic of the conditions that many natural populations may have to face under climate change.
Effect of jasmonic acid and salicylic acid induced resistance in groundnut on Helicoverpa armigera
Authors: War Abdul R. , Sharma Hari C. 
Published: April 2014

AbstractInduced resistance in plants affects insect growth and development as a result of the up‐regulation of defence‐related secondary metabolites or enzyme‐binding proteins. In the present study, the effects of jasmonic acid (JA) and salicylic acid (SA) induced resistance in groundnut on Helicoverpa armigera (Hübner) are examined. Larval survival, larval weights and the activities of digestive enzymes (total serine protease and trypsin) and of detoxifying enzymes [glutathione S‐transferase (GST) and esterase (EST)] are studied in insects fed on four groundnut genotypes with moderate levels of resistance to H. armigera (ICGV 86699, ICGV 86031, ICG 2271 and ICG 1697) and a susceptible genotype (JL 24). The plants are pre‐ and/or simultaneously treated with JA and SA, and then infested with H. armigera, which are allowed to feed for 6 days. Significantly lower serine protease and trypsin activities are observed in H. armigera fed on plants treated with JA. Greater GST activity is recorded in insects fed on JA and SA treated plants, whereas EST activity is low in H. armigera larvae fed on plants treated with JA and SA. Serine proteases, trypsin and GST activities and larval weights (r = 0.74–0.95) and larval survival (r = 0.77–0.93) are positively correlated, whereas EST activity and larval weight (r = −0.55) and larval survival (r = −0.65) are negatively correlated. The results suggest that midgut digestive and detoxifying enzymes can be used as indicators of the adverse effects of constitutive and/or induced resistance in crop plants on the insect pests and the role of JA and SA in insect pest management.
Left ventricular assist device exchange for persistent infection: a case series and review of the literature
Authors: Levy D.T. , Guo Y. , Simkins J. , Puius Y.A. , Muggia V.A. , Goldstein D.J. , D'Alessandro D.A. , Minamoto G.Y. 
Published: April 2014

AbstractLeft ventricular assist device (LVAD) exchange for control of infection may be an option for the treatment of persistent and severe infections of the LVAD. Data are limited regarding the indications for device exchange, methods for exchanging infected devices, post‐exchange antimicrobial management, and outcomes of such patients. We report a series of cases in which an exchange was performed for persistent LVAD infection, review the literature on LVAD exchange and surgical techniques for these infectious complications, and suggest management strategies from a multidisciplinary perspective.