bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fission yeast mitochondria are distributed by dynamic microtubules in a motor-independent manner
Authors: Li Tianpeng , Zheng Fan , Cheung Martin , Wang Fengsong , Fu Chuanhai 
Published: June 2015

AbstractThe cytoskeleton plays a critical role in regulating mitochondria distribution. Similar to axonal mitochondria, the fission yeast mitochondria are distributed by the microtubule cytoskeleton, but this is regulated by a motor-independent mechanism depending on the microtubule associated protein mmb1p as the absence of mmb1p causes mitochondria aggregation. In this study, using a series of chimeric proteins to control the subcellular localization and motility of mitochondria, we show that a chimeric molecule containing a microtubule binding domain and the mitochondria outer membrane protein tom22p can restore the normal interconnected mitochondria network in mmb1-deletion (mmb1∆) cells. In contrast, increasing the motility of mitochondria by using a chimeric molecule containing a kinesin motor domain and tom22p cannot rescue mitochondria aggregation defects in mmb1∆ cells. Intriguingly a chimeric molecule carrying an actin binding domain and tom22p results in mitochondria associated with actin filaments at the actomyosin ring during mitosis, leading to cytokinesis defects. These findings suggest that the passive motor-independent microtubule-based mechanism is the major contributor to mitochondria distribution in wild type fission yeast cells. Hence, we establish that attachment to microtubules, but not kinesin-dependent movement and the actin cytoskeleton, is required and crucial for proper mitochondria distribution in fission yeast.
Enhancing the antimicrobial activity of natural extraction using the synthetic ultrasmall metal nanoparticles
Authors: Li Huanhuan , Chen Quansheng , Zhao Jiewen , Urmila Khulal 
Published: June 2015

AbstractThe use of Catechin as an antibacterial agent is becoming ever-more common, whereas unstable and easy oxidation, have limited its application. A simple and low-energy-consuming approach to synthesize highly stable and dispersive Catechin-Cu nanoparticles(NPs) has been developed, in which the stability and dispersivity of the NPs are varied greatly with the pH value and temperature of the reaction. The results demonstrate that the optimal reaction conditions are pH 11 at room temperature. As-synthesized NPs display excellent antimicrobial activity, the survival rates of bacterial cells exposed to the NPs were evaluated using live/dead Bacterial Viability Kit. The results showed that NPs at the concentration of 10 ppm and 20 ppm provided rapid and effective killing of up to 90% and 85% of S. aureus and E. coli within 3 h, respectively. After treatment with 20 ppm and 40 ppm NPs, the bacteria are killed completely. Furthermore, on the basis of assessing the antibacterial effects by SEM, TEM and AFM, it was found the cell membrane damage of the bacteria caused by direct contact of the bacteria with the NPs was the effective mechanism in the bacterial inactivation.
Structural color printing based on plasmonic metasurfaces of perfect light absorption
Authors: Cheng Fei , Gao Jie , Luk Ting S. , Yang Xiaodong 
Published: June 2015

AbstractSubwavelength structural color filtering and printing technologies employing plasmonic nanostructures have recently been recognized as an important and beneficial complement to the traditional colorant-based pigmentation. However, the color saturation, brightness and incident angle tolerance of structural color printing need to be improved to meet the application requirement. Here we demonstrate a structural color printing method based on plasmonic metasurfaces of perfect light absorption to improve color performances such as saturation and brightness. Thin-layer perfect absorbers with periodic hole arrays are designed at visible frequencies and the absorption peaks are tuned by simply adjusting the hole size and periodicity. Near perfect light absorption with high quality factors are obtained to realize high-resolution, angle-insensitive plasmonic color printing with high color saturation and brightness. Moreover, the fabricated metasurfaces can be protected with a protective coating for ambient use without degrading performances. The demonstrated structural color printing platform offers great potential for applications ranging from security marking to information storage.
Chromosome-level genome map provides insights into diverse defense mechanisms in the medicinal fungus Ganoderma sinense
Authors: Zhu Yingjie , Xu Jiang , Sun Chao , Zhou Shiguo , Xu Haibin , Nelson David R. , Qian Jun , Song Jingyuan , Luo Hongmei , Xiang Li , Li Ying , Xu Zhichao , Ji Aijia , Wang Lizhi , Lu Shanfa , Hayward Alice , Sun Wei , Li Xiwen , Schwartz David C. , Wang Yitao , Chen Shilin 
Published: June 2015

AbstractFungi have evolved powerful genomic and chemical defense systems to protect themselves against genetic destabilization and other organisms. However, the precise molecular basis involved in fungal defense remain largely unknown in Basidiomycetes. Here the complete genome sequence, as well as DNA methylation patterns and small RNA transcriptomes, was analyzed to provide a holistic overview of secondary metabolism and defense processes in the model medicinal fungus, Ganoderma sinense. We reported the 48.96 Mb genome sequence of G. sinense, consisting of 12 chromosomes and encoding 15,688 genes. More than thirty gene clusters involved in the biosynthesis of secondary metabolites, as well as a large array of genes responsible for their transport and regulation were highlighted. In addition, components of genome defense mechanisms, namely repeat-induced point mutation (RIP), DNA methylation and small RNA-mediated gene silencing, were revealed in G. sinense. Systematic bioinformatic investigation of the genome and methylome suggested that RIP and DNA methylation combinatorially maintain G. sinense genome stability by inactivating invasive genetic material and transposable elements. The elucidation of the G. sinense genome and epigenome provides an unparalleled opportunity to advance our understanding of secondary metabolism and fungal defense mechanisms.
Quantum Coherent Feedback Control for Generation System of Optical Entangled State
Authors: Zhou Yaoyao , Jia Xiaojun , Li Fang , Yu Juan , Xie Changde , Peng Kunchi 
Published: June 2015

AbstractThe non-measurement based coherent feedback control (CFC) is a control method without introducing any backaction noise into the controlled system, thus is specially suitable to manipulate various quantum optical systems for preparing nonclassical states of light. By simply tuning the transmissivity of an optical controller in a CFC loop attached to a non-degenerate optical parametric amplifier (NOPA), the quantum entanglement degree of the output optical entangled state of the system is improved. At the same time, the threshold pump power of the NOPA is reduced also. The experimental results are in reasonable agreement with the theoretical expectation.
Evidence for Autoinduction and Quorum Sensing in White Band Disease-Causing Microbes on Acropora cervicornis
Authors: Certner Rebecca H. , Vollmer Steven V. 
Published: June 2015

AbstractCoral reefs have entered a state of global decline party due to an increasing incidence of coral disease. However, the diversity and complexity of coral-associated bacterial communities has made identifying the mechanisms underlying disease transmission and progression extremely difficult. This study explores the effects of coral cell-free culture fluid (CFCF) and autoinducer (a quorum sensing signaling molecule) on coral-associated bacterial growth and on coral tissue loss respectively. All experiments were conducted using the endangered Caribbean coral Acropora cervicornis. Coral-associated microbes were grown on selective media infused with CFCF derived from healthy and white band disease-infected A. cervicornis. Exposure to diseased CFCF increased proliferation of Cytophaga-Flavobacterium spp. while exposure to healthy CFCF inhibited growth of this group. Exposure to either CFCF did not significantly affect Vibrio spp. growth. In order to test whether disease symptoms can be induced in healthy corals, A. cervicornis was exposed to bacterial assemblages supplemented with exogenous, purified autoinducer. Incubation with autoinducer resulted in complete tissue loss in all corals tested in less than one week. These findings indicate that white band disease in A. cervicornis may be caused by opportunistic pathogenesis of resident microbes.
De novo Assembly and Characterization of the Testis Transcriptome and Development of EST-SSR Markers in the Cockroach Periplaneta americana
Authors: Chen Wan , Liu Yu-Xiang , Jiang Guo-Fang 
Published: June 2015

AbstractThe cockroach Periplaneta americana is a notorious pest and threat to health worldwide, with a high reproductive ability. However, a limited amount of data is available on the developmental stage-specific transcriptomes of P. americana. To identify genes involved in developmental processes and to develop additional SSR markers in P. americana, we carried out de novo assembly of the P. americana transcriptome using Illumina sequencing. After removing low-quality sequences, we obtained 64,954,709 contigs, which were further assembled into 125,390 unigenes with an average length of 711 bp. Based on similarity searches against known proteins, we identified 48,300 unigenes based on a cut-off E-value of 10−5. The assembled sequences were annotated according to gene descriptions, gene ontology and clusters of orthologous groups. A total of 14,195 potential SSRs were identified and 41 of 63 randomly chosen primer pairs successfully amplified the predicted SSR markers, seven of which were polymorphic in size in P. americana. Furthermore, the Spag6 gene was confirmed to be testes specific and the fru and RPSA genes were related to the development of the testis. This is the special report of a P. americana transcriptome obtained using Illumina sequencing technology and a large number of molecular markers were developed.
Copper uptake by DMT1: a compensatory mechanism for CTR1 deficiency in human umbilical vein endothelial cells
Authors: Lin Chen , Zhang Zhen , Wang Tao , Chen Chen , James Kang Y 
Published: June 2015

Abstract
Copper transport 1 (CTR1) plays a critical role in copper uptake by cells, but several studies demonstrated that divalent metal transporter 1 (DMT1) also transports copper in some cells and under certain circumstances. The present study was undertaken to determine the relationship between CTR1 and DMT1 in copper uptake. Human umbilical vein endothelial cells (HUVECs) were exposed to increasing concentrations of extracellular copper in cultures, leading to increased accumulation of copper in cells proportional to concentrations of extracellular copper. However, CTR1 proteins decreased in relation to the increase in copper concentrations, and DMT1 increased inversely correlating to the decrease in CTR1. Gene silencing of either CTR1 or DMT1 did not affect copper accumulation in cells, but deficiency in both CTR1 and DMT1 resulted in a complete inhibition of copper uptake. This study thus demonstrates that DMT1 imports copper under the condition of CTR1 deficiency, and vice versa. Therefore, CTR1 and DMT1 would compensate for each other for copper uptake in mammalian cells, although different types of cells may use either one as a predominant copper importer under physiological conditions.
Southeast Alaska Sperm Whale Avoidance Project (SEASWAP): a successful collaboration among scientists and industry to study depredation in Alaskan waters
Authors: Straley Janice , O'Connell Victoria , Liddle Joe , Thode Aaron , Wild Lauren , Behnken Linda , Falvey Dan , Lunsford Chris 
Published: June 2015

AbstractIn Alaskan waters, depredation on sablefish longline gear by sperm whales increases harvesting cost, negatively biases stock assessments, and presents a risk of entanglement for whales. The Southeast Alaska Sperm Whale Avoidance Project (SEASWAP), a collaborative effort involving industry, scientists, and managers, since 2003 has undertaken research to evaluate depredation with a goal of recommending measures to reduce interactions. Prior to 2003, little was known about sperm whale distribution and behaviour in the Gulf of Alaska (GOA). Although fishers were reporting increasing interactions, the level of depredation varied with no apparent predictor of occurrence across vessels. Between 2003 and 2007, fishers were provided with fishery logbooks and recorded information on whale behaviour, whale presence and absence, during the set, soak, and haul for 319 sets in the GOA. Data were evaluated for a vessel, area, and seasonal (month) effect in the presence and absence of sperm whales. Using catch per unit effort (cpue) as a metric, in kg/100 hooks, results indicated that depredation depended on both the vessel and the area. More whales associated with vessels from April to August. Sperm whales were also likely to be present when cpue was high, revealing that whales and fishers both knew the most productive fishing areas, but confounding the use of cpue as a metric for depredation. Using a Bayesian mark-recapture analysis and the sightings histories of photo-identified whales, an estimated Nˆ=135 (95% CI 124, 153) sperm whales were associating with vessels in 2014. A spatial model was fitted to 319 longline sets and quantified a 3% loss in cpue, comparable to other global studies on sperm whale depredation. Through all phases of SEASWAP, our understanding of depredation has gained significantly. This successful collaboration should be considered as a model to create partnerships and build collaborations between researchers and fisherpeople encountering marine mammal interactions with fishing gear.
Serum Immunoglobulin A Cross-Strain Blockade of Human Noroviruses
Authors: Lindesmith Lisa C. , Beltramello Martina , Swanstrom Jesica , Jones Taylor A. , Corti Davide , Lanzavecchia Antonio , Baric Ralph S. 
Published: June 2015

Abstract
Background.  Human noroviruses are the leading cause of acute viral gastroenteritis, justifying vaccine development despite a limited understanding of strain immunity. After genogroup I (GI).1 norovirus infection and immunization, blockade antibody titers to multiple virus-like particles (VLPs) increase, suggesting that GI cross-protection may occur.
Methods.  Immunoglobulin (Ig)A was purified from sera collected from GI.1-infected participants, and potential neutralization activity was measured using a surrogate neutralization assay based on antibody blockade of ligand binding. Human and mouse monoclonal antibodies (mAbs) were produced to multiple GI VLPs to characterize GI epitopes.
Results.  Immunoglobulin A purified from day 14 post-GI.1 challenge sera blocked binding of GI.1, GI.3, and GI.4 to carbohydrate ligands. In some subjects, purified IgA preferentially blocked binding of other GI VLPs compared with GI.1, supporting observations that the immune response to GI.1 infection may be influenced by pre-exposure history. For other subjects, IgA equivalently blocked multiple GI VLPs. Only strain-specific mAbs recognized blockade epitopes, whereas strain cross-reactive mAbs recognized nonblockade epitopes.
Conclusions.  These studies are the first to describe a functional role for serum IgA in norovirus immunity and the first to characterize human monoclonal antibodies to GI strains, expanding our understanding of norovirus immunobiology.
Subfamily-Specific Fluorescent Probes for Cysteine Proteases Display Dynamic Protease Activities during Seed Germination
Authors: Lu Haibin , Chandrasekar Balakumaran , Oeljeklaus Julian , Misas-Villamil Johana C. , Wang Zheming , Shindo Takayuki , Bogyo Matthew , Kaiser Markus , van der Hoorn Renier A.L. 
Published: June 2015

Abstract
Cysteine proteases are an important class of enzymes implicated in both developmental and defense-related programmed cell death and other biological processes in plants. Because there are dozens of cysteine proteases that are posttranslationally regulated by processing, environmental conditions, and inhibitors, new methodologies are required to study these pivotal enzymes individually. Here, we introduce fluorescence activity-based probes that specifically target three distinct cysteine protease subfamilies: aleurain-like proteases, cathepsin B-like proteases, and vacuolar processing enzymes. We applied protease activity profiling with these new probes on Arabidopsis (Arabidopsis thaliana) protease knockout lines and agroinfiltrated leaves to identify the probe targets and on other plant species to demonstrate their broad applicability. These probes revealed that most commercially available protease inhibitors target unexpected proteases in plants. When applied on germinating seeds, these probes reveal dynamic activities of aleurain-like proteases, cathepsin B-like proteases, and vacuolar processing enzymes, coinciding with the remobilization of seed storage proteins.
Integration of waqf-Islamic microfinance model for poverty reduction
Authors: Haneef Mohamed Aslam , Pramanik Ataul Huq , Mohammed Mustafa Omar , Bin Amin Md. Fouad , Muhammad Aliyu Dahiru 
Published: June 2015

Purpose
– This paper aims to develop an integrated waqf-based Islamic microfinance (IsMF) for poverty reduction in Bangladesh. Microfinance institutions (MFIs) have been constrained by the high cost of funds, high interest rate charges and poor human resource quality of the recipients. Islamic MFIs have recently evolved with the hope of overcoming these financial, ethical and human capital deficiencies faced by the conventional financial institutions. Moreover, a good number of integrated models have been proposed to enhance the role played by Islamic MFIs. Most of these models, however, lack empirical justifications.

Design/methodology/approach
– The research uses survey techniques. A total of 381 respondents were included in the survey. The integrated waqf-based Islamic microfinance model (IWIMM) was earlier on developed using literature and intellectual discussions. There are six constructs presenting the IWIMM, namely, waqf resources, IsMF, takaful, project financing, human resource development and poverty alleviation. In the survey instrument, 45 items represent the six constructs, but only 26 items have been retained after factor analysis. Structural equation modelling has been adopted to examine the relationship among the constructs.

Findings
– The results show that there are significant relationships between IsMF and takaful, waqf resources and human resource development, takaful and human resource development, IsMF and human resource development and, waqf resources and project financing. The results also indicate that poverty alleviation is possible through the integration of these constructs.

Research limitations/implications
– Though the paper has studied conventional and Islamic MFIs in Bangladesh, one of the populated Organisation of Islamic Cooperation (OIC) member countries and also where poverty incidence is high, further studies need to be conducted in other OIC member countries to adopt the model in line with practical and regulatory environment of those countries. Similarly, the study is based on the perception of the respondents, which limits the generalization of the result.

Practical implications
– The paper proposed a model that has the potential of being applied for poverty alleviation programmes in most of the OIC member states.

Originality/value
– The present paper has developed an IWIMM for poverty reduction.
Women chairs in academic medicine: engendering strategic intuition
Authors: Isaac Carol , Griffin Lindsay 
Published: June 2015

Purpose– Because stereotypically masculine behaviors are required for effective leadership, examining female chairs’ leadership in academic medicine can provide insight into the complex ways in which gender impacts on their leadership practices. The paper aims to discuss this issue.Design/methodology/approach– The author interviewed three female clinical chairs and compared the findings to interviews with 28 of their faculty. Grounded theory analysis of the subsequent text gathered comprehensive, systematic, and in-depth information about this case of interest at a US top-tier academic medical center.Findings– Four of five themes from the faculty were consistent with the chair’s narrative with modifications: Prior Environment (Motivated by Excellence), Tough, Direct, Transparent (Developing Trust), Communal Actions (Creating Diversity of Opinion), and Building Power through Consensus (an “Artful Exercise”) with an additional theme, the Significance (and Insignificance) of a Female Chair. While faculty members were acutely aware of the chair’s gender, the chairs paradoxically vacillated between gender being a “non-issue” and noting that male chairs “don’t do laundry.” All three female chairs in this study independently and explicitly stated that gender was not a barrier, yet intuitively used successful strategies derived from the research literature.Originality/value– This study suggests that while their gender was highlighted by faculty, these women dismissed gender as a “non-issue.” The duality of gender for these three female leaders was both minimized and subtly affirmed.
Fiery Spirits in the context of institutional entrepreneurship in Swedish healthcare
Authors: Eriksson Nomie , Ujvari Sandor 
Published: June 2015

Purpose– Clinical governance and leadership concepts can lead to more or less successful implementations of new clinical practice. The purpose of this paper is to examine how Fiery Spirits, as institutional entrepreneurs can, working in a team, implement sustained change in hospital clinical practice.Design/methodology/approach– This paper describes two case studies, conducted at two Swedish hospitals over a period of two years, in which changes in clinical practice were implemented. In both cases, key-actors, termed Fiery Spirits, played critical roles in these changes. The authors use a qualitative approach and take an intra-organizational perspective with semi-structured in-depth interviews and document analysis.Findings– The new clinical practices were successfully implemented with a considerable influence of the Fiery Spirits who played a pivotal role in the change efforts. The Fiery Spirits persuasively, based on their structural and normative legitimacy and the adoption of learning processes, advocated, and supported change.Practical implications– Fiery Spirits, given flexibility and opportunity, can be powerful forces for change outside the trajectory of management-inspired and management-directed change. Team members, when inspired and encouraged by Fiery Spirits, are less resistant to change and more willing to test new clinical practices.Originality/value– The paper complements literature on how the Fiery Spirit concept aligns with concepts of clinical governance and leadership and how change can be achieved. Additionally, the findings show the effects of legitimacy and learning processes on change in clinical practice.
Accepting the challenges of social media research
Authors: Weller Katrin 
Published: June 2015

Purpose– The purpose of this paper is to introduce a new viewpoint series, Monitoring the Media: Spotlight on Social Media Research, by providing an overview of the key challenges in social media research and some current initiatives in addressing them.Design/methodology/approach– The paper considers publication output from disciplines dealing with social media studies and summarises the key challenges as discussed in the broader research community.Findings– The paper suggests that challenges originate both from the interdisciplinary nature of social media research and from the ever-changing research landscape. It concludes that, whilst the community is addressing some challenges, others require more attention.Originality/value– The paper summarises key challenges of social media and will be of interest to researchers in different disciplines, as well as a general audience, wanting to learn about how social media data are used for research.