bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quantifying autophagosomes and autolysosomes in cells using imaging flow cytometry
Authors: Rajan Robin , Karbowniczek Magdalena , Pugsley Haley R. , Sabnani Manoj K. , Astrinidis Aristotelis , La‐Beck Ninh M. 
Published: February 2015

AbstractAutophagy dysregulation has been implicated in numerous diseases and many therapeutic agents are known to modulate this pathway. Therefore, the ability to accurately monitor autophagy is critical to understanding its role in the pathogenesis and treatment of many diseases. Recently an imaging flow cytometry method measuring colocalization of microtubule associated protein 1B light chain 3 (LC3) and lysosomal signals via Bright Detail Similarity (BDS) was proposed which enabled evaluation of autophagic processing. However, since BDS only evaluates colocalization of LC3 and lysosomal signals, the number of autophagy organelles was not taken into account. We found that in cells classified as having Low BDS, there was a large degree of variability in accumulation of autophagosomes. Therefore, we developed a new approach wherein BDS was combined with number of LC3+ puncta, which enabled us to distinguish between cells having very few autophagy organelles versus cells with accumulation of autophagosomes or autolysosomes. Using this method, we were able to distinguish and quantify autophagosomes and autolysosomes in breast cancer cells cultured under basal conditions, with inhibition of autophagy using chloroquine, and with induction of autophagy using amino acid starvation. This technique yields additional insight into autophagy processing making it a useful supplement to current techniques. © 2015 International Society for Advancement of Cytometry
QoS‐driven resource allocation in green OFDMA wireless networks
Authors: Sinaie Mahnaz , Azmi Paeiz 
Published: February 2015

SummaryIn this paper, we present a QoS‐driven energy efficient power and subcarrier allocation in green orthogonal frequency division multiple access networks. Our proposed scheme aims at maximizing the effective energy efficiency (EEE), defined as overall effective capacity per total power consumption, subject to given user delay‐QoS requirements and an average sum transmit power constraints. We analytically obtain the uniquely global optimal power and subcarrier using fractional programming, which transforms the fractional program into an equivalent parametric convex program that has a tractable solution. Analytical results show that the optimal solution has the same structure as effective capacity maximization and overall power minimization solutions. Simulation results show that there exists a trade‐off between EEE and delay. When the delay is more stringent, the EEE decreases. Moreover, when the number of subcarriers increase, EEE increases, where a fixed optimal EEE can be achieved regardless of stringency of delay‐QoS requirements. Furthermore, our proposed resource allocation provides significant EEE gains over both the independent resource allocation and overall power minimization schemes. Copyright © 2015 John Wiley & Sons, Ltd.
A single dose of S‐ketamine induces long‐term antidepressant effects and decreases oxidative stress in adulthood rats following maternal deprivation
Authors: Réus Gislaine Z. , Carlessi Anelise S. , Titus Stephanie E. , Abelaira Helena M. , Ignácio Zuleide M. , da Luz Jaine R. , Matias Beatriz I. , Bruchchen Livia , Florentino Drielly , Vieira Andriele , Petronilho Fabricia , Quevedo João 
Published: February 2015

ABSTRACTKetamine, an antagonist of N‐methyl‐d‐aspartate receptors, has produced rapid antidepressant effects in patients with depression, as well as in animal models. However, the extent and duration of the antidepressant effect over longer periods of time has not been considered. This study evaluated the effects of single dose of ketamine on behavior and oxidative stress, which is related to depression, in the brains of adult rats subjected to maternal deprivation. Deprived and nondeprived Wistar rats were divided into four groups nondeprived + saline; nondeprived + S‐ketamine (15 mg/kg); deprived + saline; deprived + S‐ketamine (15 mg/kg). A single dose of ketamine or saline was administrated during the adult phase, and 14 days later depressive‐like behavior was assessed. In addition, lipid damage, protein damage, and antioxidant enzyme activities were evaluated in the rat brain. Maternal deprivation induces a depressive‐like behavior, as verified by an increase in immobility and anhedonic behavior. However, a single dose of ketamine was able to reverse these alterations, showing long‐term antidepressant effects. The brains of maternally deprived rats had an increase in protein oxidative damage and lipid peroxidation, but administration of a single dose of ketamine reversed this damage. The activities of antioxidant enzymes superoxide dismutase and catalase were reduced in the deprived rat brains. However, ketamine was also able to reverse these changes. In conclusion, these findings indicate that a single dose of ketamine is able to induce long‐term antidepressant effects and protect against neural damage caused by oxidative stress in adulthood rats following maternal deprivation. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1268–1281, 2015
Apps and eating disorders: A systematic clinical appraisal
Authors: Fairburn Christopher G. , Rothwell Emily R. 
Published: February 2015

ABSTRACTObjectiveSmartphone applications (apps) are proliferating and health‐related apps are particularly popular. The aim of this study was to identify, characterize, and evaluate the clinical utility of apps designed either for people with eating disorders or for eating disorder professionals.MethodA search of the major app stores identified 805 potentially relevant apps, of which 39 were primarily designed for people with eating disorders and five for professionals.ResultsThe apps for people with eating disorders had four main functions. Most common was the provision of advice, the quality of which ranged from sound to potentially harmful. Five apps included self‐assessment tools but only two used methods that would generally be viewed as reliable. Four apps had the self‐monitoring of eating habits as a major feature. Entering information into these apps could be accomplished with varying degrees of ease, but viewing it was more difficult. One app allowed the transfer of information between patients and clinicians.DiscussionThe enthusiasm for apps outstrips the evidence supporting their use. Given their popularity, it is suggested that clinicians evaluate app use as part of routine assessment. The clinical utility of the existing apps is not clear. Some are capable of tracking key features over time, but none has the functions required for analytic self‐monitoring as in cognitive behavioral treatments. The full potential of apps has yet to be realized. Specialized apps could be designed to augment various forms of treatment, and there is the possibility that they could deliver an entire personalized intervention. © 2015 The Authors. International Journal of Eating Disorders published by Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:1038–1046)
Luminescence Matching with the Sensitivity Curve of the Human Eye: Optical Ceramics Mg8–xMx(BN2)2N4 with M = Al (x = 2) and M = Si (x = 1)
Authors: Ströbele Markus , Dolabdjian Konstantin , Enseling David , Dutczak Danuta , Mihailova Boriana , Jüstel Thomas , Meyer H.‐Jürgen 
Published: February 2015

AbstractCompounds Mg8–xMx(BN2)2N4 were prepared by solid‐state reaction of MgCl2, Li3(BN2), and AlN for M = Al, and of Mg3N2, BN, and Si3N4 for M = Si. Their crystal structures were refined from powder X‐ray diffraction data with the space group Rm for M = Al, Si, and P21/c for M = Si. The structures of Mg8–xMx(BN2)2N4 are characterized by layered arrangements of wurtzite‐related block layers alternating with a layer of di‐nitridoborate ions along the c‐axis direction. Cations in each structure are surrounded tetrahedrally by nitride and di‐nitridoborate ions. Europium(II)‐doped powders of Mg8–xMx(BN2)2N4 show exceptional broad band emissions in the visible region of the spectrum, on excitation with UV radiation at λex = 320 nm.
On the Thermal Stability and Structures of Layered Double Hydroxides Mg1–xAlx(OH)2(NO3)x·mH2O (0.18 ≤ x ≤ 0.38)
Authors: Sjåstad Anja Olafsen , Andersen Niels Højmark , Vajeeston Ponniah , Karthikeyan Jayakumar , Arstad Bjørnar , Karlsson Arne , Fjellvåg Helmer 
Published: February 2015

AbstractNitrate‐based layered double hydroxides, Mg1–xAlx(OH)2(NO3)x·mH2O, have been investigated to settle issues relating to stability towards humidity, dehydration and staging, nitrate group orientation, Al migration and crystal structure. Raman and IR spectroscopy together with modelling methods proved that flat‐lying water and nitrate become tilted towards [001] at higher nitrate concentrations, accompanied by expansion along the c‐axis. The distribution of the molecular tilts is greatest for x = 0.25, which explains the extraordinary broad diffraction peaks. The unit cell volume was modified for all samples when subjected to moist air, most strongly for x = 0.25. On heating, in situ synchrotron diffraction data showed continuous changes along the c‐axis during dehydration of samples with x = 0.20 and 0.33. For x = 0.25, an intermediate phase appeared. Dehydroxylation was initiated at the OH groups bonded to the cations MgAl2, Mg2Al and Mg3 (in said order), whereas complete nitrate loss occurred at higher temperatures for higher Al contents. Some aluminium simultaneously moved to tetrahedral sites, which, according to 1H–27Al CP NMR spectroscopy, lack protons in their near neighbourhood. In contrast, octahedral aluminium remains surrounded by protons during decomposition. For the metastable oxide product, neutron diffraction indicated that 2/3 of aluminium reside in tetrahedral interstices. This oxide is defective, probably mimicking wüstite rather than a regular rock salt structure.
Evaluation of the short term 12 hour toxicity of 3-trifluoromethyl-4-nitrophenol (TFM) to multiple life stages of Venustaconcha ellipsiformis and Epioblasma triquetra and its host fish (Percina caprodes)
Authors: Boogaard Michael A. , Newton Teresa J. , Hubert Terrance D. , Kaye Cheryl A. , Barnhart M. Christopher 
Published: February 2015

Abstract

The present study evaluated the risk of 12-h exposures of the lampricide 3-trifluoromethyl-4-nitrophenol (TFM) to multiple life stages of the federally endangered snuffbox (Epioblasma triquetra) and its primary host fish the common logperch (Percina caprodes) as well as a surrogate to the snuffbox, the ellipse (Venustaconcha ellipsiformis). Life stages examined included free glochidia, 1-wk juveniles, and adults of the ellipse; free glochidia, glochidia on host fish, and 1-wk juveniles of the snuffbox; and adult logperch. Larval sea lampreys were also tested alongside adult ellipse and logperch for direct comparison. Survival exceeded 82% among all life stages in both mussel species at levels up to 1.8 times what would be applied during treatments, suggesting that routine sea lamprey control operations would not adversely affect mussels. However, substantial mortality of adult logperch was observed at TFM concentrations typically applied to streams, and loss of host fish could adversely affect snuffbox reproduction. In addition, TFM had no significant effect on the number of glochidia that metamorphosed on adult logperch. Although the snuffbox is not likely to be acutely affected from sea lamprey control operations, mitigation efforts to minimize impacts to the host fish should be considered. Environ Toxicol Chem 2015;34:1634–1641. © 2015 SETAC
Panel: Fundamentals of egress calculations for life safety for egress assessments
Authors: Boyce Karen 
Published: February 2015

SummaryThe purpose of this panel was to allow data users, that is, consultants and engineers, who assess the life safety of structures and regulators who approve these assessments the opportunity to discuss the current state of egress analysis from their perspectives. In other words, what datasets do engineers/consultants consistently use to assess the life safety of structures? How useful are these data and what additional data should/could data collectors and model developers provide to them to improve the quality of their work in the field? From this panel discussion, an egress roadmap for future research could be developed that would aid researchers in the field of human behaviour in fire to collect data and produce models that better support data users' needs and requirements. The format of the panel session was a brief response by engineering consultants, a data collector and an evacuation modeller to a series of questions related to the current status of egress analysis, followed by a facilitated open floor discussion on issues arising. The panel session was audio taped and the summary that follows, although not a verbatim account, is a close reflection of the views expressed and issues raised. Copyright © 2015 John Wiley & Sons, Ltd.
Tailoring Electrochemical Property of Layered Perovskite Cathode by Cu‐doping for Proton‐Conducting IT‐SOFCs
Authors: Ling Y. , Zhao L. , Liu X. , Lin B. 
Published: February 2015

AbstractLayered perovskite oxide YBaCuCoO5+x (YBCC) was synthesized by an EDTA‐citrate complexation process and was investigated as a novel cathode for proton‐conducting intermediate temperature solid oxide fuel cells (IT‐SOFCs). The thermal expansion coefficient (TEC) of YBCC was 15.3 × 10−6 K−1 and the electrical conductivity presented a semiconductor‐like behavior with the maximum value of 93.03 Scm−1 at 800 °C. Based on the defect chemistry analysis, the electrical conductivity gradually decreases by the introduction of Cu into Co sites of YBaCo2O5+x and the conductor mechanism can transform from the metallic‐like behavior to the semiconductor‐like behavior. Thin proton‐conducting (BaZr0.1Ce0.7Y0.1Yb0.1O3–δ) BZCYYb electrolyte and NiO–BZCYYb anode functional layer were prepared over porous anode substrates composed of NiO–BZCYYb by a one‐step dry‐pressing/co‐firing process. Laboratory‐sized quad‐layer cells of NiO‐BZCYYb / NiO‐BZCYYb / BZCYYb / YBCC with a 20 μm‐thick BZCYYb electrolyte membrane exhibited the maximum power density as high as 435 mW cm−2 with an open‐circuit potential (OCV) of 0.99 V and a low interfacial polarization resistance of 0.151 Ωcm2 at 700 °C. The experimental results have indicated that the layered perovskite oxide YBCC can be a cathode candidate for utilization as proton‐conducting IT‐SOFCs.
Heat and cold waves trends in the Carpathian Region from 1961 to 2010
Authors: Spinoni Jonathan , Lakatos Monika , Szentimrey Tamás , Bihari Zita , Szalai Sandor , Vogt Jürgen , Antofie Tiberiu 
Published: February 2015

ABSTRACTThe past two decades of the 20th century and the first of the 21st century have been characterized by global temperature rise and increased frequency of weather‐induced extreme events such as floods, droughts, heavy rainfall, and heat waves. We investigated the heat and the cold waves in the Carpathian Region, an area whose rich biosphere is endangered by extreme events. We used the daily minimum (TN) and maximum (TX) temperature data collected in the framework of the CARPATCLIM project. Such high‐resolution (0.1° × 0.1°) gridded data range from January 1961 to December 2010. In this study, a heat wave occurs when temperature is above the 90th percentile for at least five consecutive days and a cold wave occurs when temperature is below the 10th percentile for at least five consecutive days. The percentiles have been computed over the baseline period 1971–2000. We distinguish between night‐time and daytime events and we discuss heat (and cold) waves considering at least five consecutive night and days with temperature above (below) the selected percentile. For each heat or cold wave event, we assigned duration, severity, and intensity. For these parameters and for frequency, we performed linear trend analysis for the period 1961–2010. The trends have been computed on an annual and seasonal basis and tested for statistical significance. Different spatial patterns of heat and cold waves characterize the Carpathian Region: heat wave events show general increase in all the parameters considered, while cold wave events show a decrease in all the variables West to the Carpathians and an increase North–East to the Carpathians. We also compiled a list of the most relevant heat waves that hit the Carpathian Region from 1961 to 2010: out of seven events, four occurred from 2000 to 2010. Instead, the 1960s and the 1980s have been the decades most hit by severe cold waves.
Expression of intercellular adhesion molecule‐1 by myofibers in mdx mice
Authors: Torres‐Palsa Maria J. , Koziol Matthew V. , Goh Qingnian , Cicinelli Peter A. , Peterson Jennifer M. , Pizza Francis X. 
Published: February 2015

ABSTRACTIntroduction: We investigated the extent to which intercellular adhesion molecule‐1 (ICAM‐1), a critical protein of the inflammatory response, is expressed in skeletal muscles of mdx mice (a murine model of Duchenne muscular dystrophy). Methods: Muscles were collected from control and mdx mice at 2‐24 weeks of age and analyzed for ICAM‐1 expression by means of Western blot and immunofluorescence. Results: Western blot revealed higher expression of ICAM‐1 in mdx compared with control muscles through 24 weeks of age. In contrast to control muscles, ICAM‐1 was expressed on the membrane of damaged, regenerating, and normal myofibers of mdx mice. CD11b+ myeloid cells also expressed ICAM‐1 in mdx muscles, and CD11b+ cells were closely associated with the membrane of myofibers expressing ICAM‐1. Conclusions: These findings support a paradigm in which ICAM‐1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology. Muscle Nerve 52: 795–802, 2015
The temperature dependence of the luminescence of rare‐earth‐doped semiconductors: 25 years after Favennec
Authors: O'Donnell K. P. 
Published: February 2015

AbstractTwentyfive years after the publication of P. N. Favennec's seminal paper on luminescence from rare‐earth‐doped semiconductors (Electron. Lett. 25, 718–719 (1989), with 390+ citations to date) we examine the long shadow it has cast on recent studies of europium‐doped GaN, aimed at substituting for InN‐rich InGaN in red‐light‐emitting devices (LEDs). According to Favennec's principle, wider band gap semiconductors should show weaker thermal quenching, thus favouring the III‐nitrides hugely. The conventional approach to fitting temperature dependences of light emission, based on competition between radiative and non‐radiative transitions, is presented here in simplified form and an alternative fitting equation proposed. The original data of Favennec (op. cit.) is re‐examined in the light of these fitting models. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Selecting Texts and Tasks for Content Area Reading and Learning
Authors: Fisher Douglas , Frey Nancy 
Published: February 2015

AbstractFor students to learn science, social studies, and technical subjects, their teachers have to engage them in meaningful lessons. As part of those lessons, students read informational texts. The selection of those texts is critical. Teachers can select texts worthy of attention and then align instruction and the post‐reading tasks such that students learn at higher levels.
Enhancing Core Reading Programs With Culturally Responsive Practices
Authors: Toppel Katie 
Published: February 2015

AbstractCulturally responsive instruction uses, “cultural knowledge, prior experiences, frames of reference, and performance styles of ethnically diverse students to make learning encounters more relevant to and effective for them.” Because approximately 75% of districts choose to use one of the various publisher‐created reading programs, it is imperative for teachers to understand how to utilize culturally responsive teaching practices within the context of such programs. The components of culturally responsive instruction can be addressed within practices many teachers already use while implementing core reading programs (i.e. demonstrating care for students, incorporating opportunities for student collaboration, and strategically using instructional techniques to elicit better engagement). This article provides teachers with specific examples to illustrate how cultural responsiveness can be achieved when teachers thoughtfully consider the learning and communication styles of culturally and linguistically diverse students when planning cooperative learning and engagement strategies.
Reducing Transmission Risk Through High-Level Disinfection of Transvaginal Ultrasound Transducer Handles
Authors: Ngu Andrew , McNally Glenn , Patel Dipika , Gorgis Vivian , Leroy Sandrine , Burdach Jon 
Published: February 2015

Intracavity ultrasound transducer handles are not routinely immersed in liquid high-level disinfectants. We show that residual bacteria, including pathogens, persist on more than 80% of handles that are not disinfected, whereas use of an automated device reduces contamination to background levels. Clinical staff should consider the need for handle disinfection.Infect Control Hosp Epidemiol 2015;00(0): 1–4
Carrots and Sticks: Achieving High Healthcare Personnel Influenza Vaccination Rates without a Mandate
Authors: Drees Marci , Wroten Kathleen , Smedley Mary , Mase Tabe , Schwartz J. Sanford 
Published: February 2015

OBJECTIVEAchieving high healthcare personnel (HCP) influenza vaccination rates has typically required mandating vaccination, which is often challenging to implement. Our objective was to achieve >90% employee influenza vaccination without a mandate.DESIGNProspective quality improvement initiativeSETTING AND PARTICIPANTSAll employees of a 2-hospital, 1,100-bed, community-based academic healthcare system.METHODSThe multimodal HCP vaccination campaign consisted of a mandatory declination policy, mask-wearing for non-vaccinated HCP, highly visible “I’m vaccinated” hanging badges, improved vaccination tracking, weekly compliance reports to managers and vice presidents, disciplinary measures for noncompliant HCP, vaccination stations at facility entrances, and inclusion of a target employee vaccination rate (>75%) metric in the annual employee bonus program. The campaign was implemented in the 2011–2012 influenza season and continued throughout the 2012–2013 through 2014–2015 influenza seasons. Employee compliance, vaccination, exemption and declination rates were calculated and compared with those of the seasons prior to the intervention.RESULTSCompared with vaccination rates of 57%–72% in the 3 years preceding the intervention, employee influenza vaccination increased to 92% in year 1 and 93% in years 2–4 (P<.001). The proportion of employees declaring medical/religious exemptions or declining vaccination decreased during the 4 years of the program (respectively, 1.2% to 0.5%,P<.001; 4.4% to 3.8%,P=.001).CONCLUSIONSAn integrated multimodal approach incorporating peer pressure, accountability, and financial incentives was associated with increased employee vaccination rate from ≤72% to ≥92%, which has been sustained for 4 influenza seasons. Such programs may provide a model for behavioral change within healthcare organizations.Infect Control Hosp Epidemiol2015;00(0): 1–8