bluuebunny/crossref_metadata_2025_split huggingface dataset:
PAS‐G supports platelet reconstitution after cryopreservation in the absence of plasma
Authors: Johnson Lacey , Reid Samantha , Tan Shereen , Vidovic Diana , Marks Denese C. 
Published: January 2013

BackgroundPlatelet (PLT) concentrates frozen in dimethyl sulfoxide (DMSO) can be stored for extended periods at −80°C. PLTs are frozen in a hyperconcentrated state to avoid postthaw washing and minimize residual DMSO. Consequently, PLTs require reconstitution upon thawing. Although plasma, saline, and PLT additive solutions (PASs) have been used to reconstitute frozen PLTs, a comparison to define an optimal solution for PLT recovery has not been performed.Study Design and MethodsDMSO (5% final concentration) was added to buffy coat–derived PLTs, followed by centrifugation to concentrate and freezing at −80°C. Cryopreserved PLTs (n = 12 per group) were thawed at 37°C, reconstituted in a unit of thawed frozen plasma, SSP+, or PAS‐G. In vitro PLT quality was examined before freezing, immediately after thawing, and 6 and 24 hours after thawing.ResultsAfter thawing and reconstitution, PLTs in plasma and PAS‐G displayed similar recovery (69 and 73%, respectively), while PLT recovery in SSP+ was lower (62%). All PLTs maintained an acceptable pH and metabolic activity during postthaw storage. Frozen PLTs were activated, although the extent differed depending on the reconstitution solution, with PLTs in PAS‐G retaining better aggregation responses than PLTs in plasma or SSP+.ConclusionThawing cryopreserved PLTs in PAS‐G, without plasma supplementation, resulted in PLTs with similar recovery and in vitro quality indicators as those suspended in plasma. Importantly, using PAS‐G enables the PLTs to be ready for use significantly faster than when having to thaw frozen plasma, which may be beneficial in trauma situations.
Long‐term clinical outcomes after Streptococcus bovis isolation in asymptomatic blood donors in Hong Kong
Authors: Lee C.K. , Chan H.M.H. , Ho P.L. , Wong H.K. , Leung J.N.S. , Tsoi W.C. , Lin C.K. 
Published: January 2013

BackgroundRisk of transfusion‐transmitted bacterial sepsis has been substantially reduced by a bacterial surveillance program (BST). However, new problems emerge as asymptomatic bacteremia is detected in blood donors. Streptococcus bovis bacteremia, which is known to associate with infective endocarditis and colonic carcinoma, is an example.Study Design and MethodsA retrospective study was conducted to examine the demographic and clinical outcome of this group of donors. All confirmed culture‐positive cases under the BST were retrieved and those donors with S. bovis bacteremia were contacted for follow‐up. Viable culture samples were sent for detailed microbiologic analysis.ResultsFrom 1998 to 2012, a total of 16 donors were found to have S. bovis bacteremia, giving an estimated prevalence of 1 in 110,800 donations. They consisted of nine men and seven women with median age of 43.5 years. Eight donors had undergone colonoscopy with colonic carcinoma detected in two and benign adenoma in four. Five of the 16 isolates could be retrieved for 16S DNA sequencing. Four were identified as S. gallolyticus ssp. pasteurianus and one as S. gallolyticus ssp. gallolyticus. The two patients with colonic carcinoma had one each of subspecies pasteurianus and gallolyticus.ConclusionThe findings highlight a close association of S. bovis and colonic carcinoma. We recommend prompt donor follow‐up if S. bovis bacteremia is detected. Besides, all confirmed S. bovis should be fully characterized by molecular technique.
Intravascular immune hemolysis caused by the contrast medium iomeprol
Authors: Mayer Beate , Leo Albrecht , Herziger Angela , Houben Philipp , Schemmer Peter , Salama Abdulgabar 
Published: January 2013

BackgroundImmune‐mediated hemolysis is not included in the list of adverse reactions related to contrast medium (CM). Here, we report on a patient who developed immune hemolytic anemia (IHA) related to iomeprol, a nonionic CM.Study Design and MethodsA 34‐year‐old female patient developed massive hemolysis during infusion of 50 mL of iomeprol. Serologic studies were performed using standard techniques.ResultsBefore hemolysis, the patient's serum was weakly positive with e+ red blood cells (RBCs; autoanti‐e) and the direct antiglobulin test (DAT) was negative. After hemolysis, the patient's serum samples became significantly reactive with e– RBCs in the presence of iomeprol but not in the presence of two other similar CM. The DAT became strongly positive only with anti‐C3d.ConclusionInitially, an allergic reaction was suggested, and as the hemolysis became obvious, a toxic hemolysis was suspected. However, serologic reexamination revealed an iomeprol‐dependent antibody. IHA related to CM has yet only been described in one patient in 1991. The hemolysis in that patient was related to Isopaque, an older ionic CM. Here, we describe an additional patient and recommend that CM should be considered as a rare risk in the development of IHA.
Trade and Climate Policies: Do Emissions from International Transport Matter?
Authors: Vöhringer Frank , Grether Jean‐Marie , Mathys Nicole A. 
Published: January 2013

AbstractThis paper provides orders of magnitude of the importance of CO2 emissions from international freight transport activities under a variety of scenarios regarding trade and climate policies. It is based on a stylised multiregion, multisector CGE model that includes the four modes of international transport (air, water, road and rail) and where choices regarding the energy mix and transport modes have been endogeneised. A separate decomposition of emission changes into the well‐known scale, composition and technique effects is provided. Scale effects turn out to be roughly double in international transport than in exports, while technique effects are weaker due to less substitutability between energy inputs. As a result, international transport represents half of the world increase in global emissions when trade liberalisation is considered in isolation. When trade liberalisation is coupled with a carbon tax limited to rich countries, the change in international transport emissions represents roughly one‐eighth of the carbon leakage effect.
Comparison of systemic atracurium, retrobulbar lidocaine, and sub‐Tenon's lidocaine injections in akinesia and mydriasis in dogs
Authors: Ahn Jaesang , Jeong Manbok , Park Youngwoo , Lee Yesran , Lee Euiri , Kim Soohyun , Lee Inhyung , Seo Kangmoon 
Published: January 2013

AbstractObjectiveTo compare the effect of sub‐Tenon's lidocaine injections (ST) on akinesia and mydriasis to those of systemic atracurium (AT) and retrobulbar lidocaine injections in dogs.Animal studiedTen healthy beagle dogs without apparent ocular disease.ProceduresThree treatments were performed on 10 beagle dogs with a minimum 7‐day washout period: intravenous injection of AT (0.2 mg/kg, AT group); retrobulbar (RB) injection of 2% lidocaine (2.0 mL, RB group) in one eye; and sub‐Tenon's injection of 2% lidocaine (2.0 mL, ST group) in the opposite eye. When the akinesia was not obtained within 10 min, an additional 1 mL of lidocaine was administered in the RB and the ST groups.ResultsOnset of akinesia in the AT (1.5 ± 0.9 min) and the ST (3.8 ± 5.8 min) groups was significantly shorter than that in the RB group (9.0 ± 6.5 min). Duration of akinesia in the ST group (116.2 ± 32.8 min) was longer compared to the AT (60.6 ± 23.6 min) and the RB (89.0 ± 52.8 min) groups, even though there was only a significant difference between the AT and the ST groups. Mydriasis was achieved in five eyes in the RB group and nine eyes in the ST group. There was no significant difference in onset (3.6 ± 3.1 and 2.9 ± 2.3 min, respectively) or duration (91.4 ± 31.9 and 102.1 ± 35.8 min, respectively) of mydriasis between the groups.ConclusionsSub‐Tenon's lidocaine injections provide excellent akinesia and mydriasis compared to systemic AT and retrobulbar lidocaine injections. Therefore, sub‐Tenon's anesthesia could be an alternative to the systemic administration of neuromuscular blockers and retrobulbar anesthesia for ophthalmic surgery in dogs.
Multilocus Sequence Analysis of Streptococcus canis Confirms the Zoonotic Origin of Human Infections and Reveals Genetic Exchange with Streptococcus dysgalactiae subsp.equisimilis
Authors: Pinho M. D. , Matos S. C. , Pomba C. , Lübke-Becker A. , Wieler L. H. , Preziuso S. , Melo-Cristino J. , Ramirez M. 
Published: January 2013

ABSTRACTStreptococcus canisis an animal pathogen that occasionally causes human infections. Isolates recovered from infections of animals (n= 78, recovered from 2000 to 2010 in three European countries, mainly from house pets) and humans (n= 7, recovered from 2006 to 2010 in Portugal) were identified by phenotypic and genotypic methods and characterized by antimicrobial susceptibility testing, multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE), andemmtyping.S. canisisolates presented considerable variability in biochemical profiles and 16S rRNA. Resistance to antimicrobial agents was low, with the most significant beingtet(M)- andtet(O)-mediated tetracycline resistance. MLST analysis revealed a polyclonal structure of theS. canispopulation causing infections, where the same genetic lineages were found infecting house pets and humans and were disseminated in distinct geographic locations. Phylogenetic analysis indicated thatS. caniswas a divergent taxon of the sister speciesStreptococcus pyogenesandStreptococcus dysgalactiaesubsp.equisimilisand found evidence of acquisition of genetic material byS. canisfromS. dysgalactiaesubsp.equisimilis. PFGE confirmed the MLST findings, further strengthening the similarity between animal and human isolates. The presence ofemm-like genes was restricted to a few isolates and correlated with some MLST-based genetic lineages, but none of the human isolates could beemmtyped. Our data show thatS. canisisolates recovered from house pets and humans constitute a single population and demonstrate that isolates belonging to the main genetic lineages identified have the ability to infect the human host, providing strong evidence for the zoonotic nature ofS. canisinfection.
Invasive Sino-Orbital Mycosis in an Aplastic Anemia Patient Caused by Neosartorya laciniosa
Authors: Malejczyk Kathy , Sigler Lynne , Gibas Connie Fe C. , Smith Stephanie W. 
Published: January 2013

ABSTRACT

We report the first case of
Neosartorya laciniosa
invasive sinusitis involving the orbit in an immunocompromised male with aplastic anemia. Treatment included surgical debridement with enucleation of the eye and combination voriconazole and micafungin therapy followed by voriconazole alone. The fungus was identified using sequencing of partial
benA
and calmodulin genes.
Rapid and Simultaneous Detection of Genes Encoding Klebsiella pneumoniae Carbapenemase (
bla
KPC
) and New Delhi Metallo-β-Lactamase (
bla
NDM
) in Gram-Negative Bacilli
Authors: Cunningham Scott A. , Noorie Tabassum , Meunier Daniele , Woodford Neil , Patel Robin 
Published: January 2013

ABSTRACT

We present a duplex, real-time PCR assay for detection of
Klebsiella pneumoniae
carbapenemase (
bla
KPC
) and New Delhi metallo-β-lactamase (
bla
NDM
) genes. Accuracy was assessed with 158 Gram-negative bacillary isolates, including 134 carbapenemase producers. The assay had 100% sensitivity and specificity compared with reference methods and a turnaround time of 90 min.
The Orchestrated Functions of Innate Leukocytes and T Cell Subsets Contribute to Humoral Immunity, Virus Control, and Recovery from Secondary Poxvirus Challenge
Authors: Tahiliani Vikas , Chaudhri Geeta , Eldi Preethi , Karupiah Gunasegaran 
Published: January 2013

ABSTRACT
A pivotal role for antigen-specific recall responses to secondary virus infection is well established, but the contribution of innate immune cells to this process is unknown. Recovery of mice from a primary orthopoxvirus (ectromelia virus [ECTV]) infection requires the function of natural killer (NK) cells, granulocytes, plasmacytoid dendritic cells (pDC), T cells, and B cells. However, during a secondary challenge, resolution of infection is thought to be dependent on antibody but not T cell function. We investigated the contribution of NK cells, granulocytes, and pDC to virus control during a secondary virus challenge in mice that had been primed with an avirulent, mutant strain of ECTV. Mice depleted of NK cells, granulocytes, or pDC effectively controlled virus, as did mice depleted of both CD4 and CD8 T cell subsets. However, mice concurrently depleted of all three innate cell subsets had elevated virus load, but this was significantly exacerbated in mice also depleted of CD4 and/or CD8 T cells. Increased viral replication in mice lacking innate cells plus CD4 T cells was associated with a significant reduction in neutralizing antibody. Importantly, in addition to T-dependent neutralizing antibody responses, the function of CD8 T cells was also clearly important for virus control. The data indicate that in the absence of innate cell subsets, a critical role for both CD4 and CD8 T cells becomes apparent and, conversely, in the absence of T cell subsets, innate immune cells help contain infection.
Susceptibility of Human Iris Stromal Cells to Herpes Simplex Virus 1 Entry
Authors: Baldwin John , Park Paul J. , Zanotti Brian , Maus Erika , Volin Michael V. , Shukla Deepak , Tiwari Vaibhav 
Published: January 2013

ABSTRACT

Ocular herpes simplex virus 1 (HSV-1) infection can lead to multiple complications, including iritis, an inflammation of the iris. Here, we use human iris stroma cells as a novel
in vitro
model to demonstrate HSV-1 entry and the inflammatory mediators that can damage the iris. The upregulated cytokines observed in this study provide a new understanding of the intrinsic immune mechanisms that can contribute to the onset of iritis.
Acquisition of the Metastatic Phenotype Is Accompanied by H2O2-Dependent Activation of the p130Cas Signaling Complex
Authors: Hempel Nadine , Bartling Toni R. , Mian Badar , Melendez J. Andres 
Published: January 2013

Abstract
Reactive oxygen species (ROS) have emerged as cellular signaling molecules and are implicated in metastatic disease by their ability to drive invasion and migration. Here, we define the signaling adaptor protein p130Cas (Crk-associated substrate) as a key redox-responsive molecular trigger that is engaged in highly invasive metastatic bladder tumor cell lines. Endogenous shifts in steady-state hydrogen peroxide (H2O2) that accompany the metastatic phenotype increase p130Cas phosphorylation, membrane recruitment and association with the scaffolding protein Crk, and subsequent Rac1 activation and actin reorganization. Both enzymatic and nonenzymatic scavenging of H2O2 abrogates p130Cas-dependent signaling and the migratory and invasive activity of the metastatic bladder tumor cells. Disruption of p130Cas attenuates both invasion and migration of the metastatic variant (253J-BV). 253J-BV cells displayed an increase in global thiol oxidation and a concomitant decrease in total phosphatase activity, common target proteins of active-site cysteine oxidation. The dependence of phosphatases on regulation of p130Cas was highlighted when depletion of PTPN12 enhanced p130cas phosphorylation and the migratory behavior of a noninvasive parental bladder tumor control (253J). These data show that the metastatic phenotype is accompanied by increases in steady-state H2O2 production that drive promigratory signaling and suggest that antioxidant-based therapeutics may prove useful in limiting bladder tumor invasiveness. Mol Cancer Res; 11(3); 303–12. ©2013 AACR.
A nanobody-based method for tracking factor XII activation in plasma
Authors: de Maat Steven , van Dooremalen Sanne , Groot Philip G. , Maas Coen 
Published: January 2013

SummaryThe physiological role of the plasma protein factor XII (FXII), as well as its involvement in human pathology, is poorly understood. While FXII is implicated in thrombotic pathology as a coagulation factor, it can contribute to inflammatory conditions without triggering coagulation. We recently generated nanobodies against the catalytic domain of activated FXII (FXIIa). Here, we describe two of these nanobodies, A10 and B7, both of which do not recognise FXII. Nanobody A10 recognises the catalytic domain of purified β-FXIIa (80 kDa), but not that of purified α-FXIIa (28 kDa), whereas nanobody B7 recognises both. This suggests minute differences in the catalytic domain between these isoforms of FXIIa. The detection of FXIIa by these nanobodies in plasma can become compromised through inactivation by serine protease inhibitors. This effect can be efficiently countered through the addition of the small-molecular protease inhibitor PPACK. Finally, we show that our nanobody-based assays in vitro distinguish various activation products of FXII that differ with the type of activator present: whereas procoagulant activators solely trigger the formation of a species that is captured by B7, proinflammatory activators first generate a species that is recognised by B7, which is later converted into a species that is recognised by A10. These findings suggest that a progressive proteolysis of FXIIa results in the generation a non-procoagulant form of FXIIa, whereas retention of intermediate forms triggers coagulation. Moreover, our findings indicate the development of nanobodies against activated enzymes offers improved opportunities to investigate their contribution to health and disease.
One Bow or Another? A Study of the Bow in Genesis 9:8-17
Authors: van Wolde Ellen 
Published: January 2013

AbstractBecause Gen. 9:8-17 uses the wordqĕšĕtin relation to the deity and to the clouds, the inference has been made in biblical scholarship that the text refers to a rainbow. The plausibility of this inference is tested in this article. Attention is given to the various linguistic aspects of this word in the Hebrew Bible and to the specific textual composition of Gen. 9:8-17 as well as to the broader ancient Near Eastern framework established by comparative literary and iconographic evidence. The conclusion is reached that the wordqĕšĕtdesignates in Gen. 9:8-17 a warrior’s bow which represents both the deity’s might and power as well as his willingness to transfer his power over the earth to those living on it.
Weeping in the Psalms
Authors: Bosworth David A. 
Published: January 2013

Abstract
The motif of weeping in the Psalter has not been a subject of previous study. Scientific study of weeping indicates that crying is a behavior motivated by the need for relationship and care-giving and that tears generate powerful reactions in those who witness them. In the Psalms, speakers draw on the powerful non-verbal eloquence of weeping by mentioning their tears in the contexts of their prayers in order to motivate God to help. This article describes how the motif of weeping functions in the Psalms and analyzes its distribution in the Psalter.
Integrin activation by P-Rex1 is required for selectin-mediated slow leukocyte rolling and intravascular crawling
Authors: Herter Jan M. , Rossaint Jan , Block Helena , Welch Heidi , Zarbock Alexander 
Published: January 2013

Key Points
P-Rex1 is involved in E-selectin-mediated slow leukocyte rolling. P-Rex1 mediates Mac-1-dependent intravascular crawling.
Antimicrobial activity, antibiotic susceptibility and virulence factors of Lactic Acid Bacteria of aquatic origin intended for use as probiotics in aquaculture
Authors: Muñoz-Atienza Estefanía , Gómez-Sala Beatriz , Araújo Carlos , Campanero Cristina , del Campo Rosa , Hernández Pablo E , Herranz Carmen , Cintas Luis M 
Published: January 2013

Abstract

Background
The microorganisms intended for use as probiotics in aquaculture should exert antimicrobial activity and be regarded as safe not only for the aquatic hosts but also for their surrounding environments and humans. The objective of this work was to investigate the antimicrobial/bacteriocin activity against fish pathogens, the antibiotic susceptibility, and the prevalence of virulence factors and detrimental enzymatic activities in 99 Lactic Acid Bacteria (LAB) (59 enterococci and 40 non-enterococci) isolated from aquatic animals regarded as human food.

Results
These LAB displayed a broad antimicrobial/bacteriocin activity against the main Gram-positive and Gram-negative fish pathogens. However, particular safety concerns based on antibiotic resistance and virulence factors were identified in the genus Enterococcus (86%) (Enterococcus faecalis, 100%; E. faecium, 79%). Antibiotic resistance was also found in the genera Weissella (60%), Pediococcus (44%), Lactobacillus (33%), but not in leuconostocs and lactococci. Antibiotic resistance genes were found in 7.5% of the non-enterococci, including the genera Pediococcus (12.5%) and Weissella (6.7%). One strain of both Pediococcus pentosaceus and Weissella cibaria carried the erythromycin resistance gene mef(A/E), and another two P. pentosaceus strains harboured lnu(A) conferring resistance to lincosamides. Gelatinase activity was found in E. faecalis and E. faecium (71 and 11%, respectively), while a low number of E. faecalis (5%) and none E. faecium exerted hemolytic activity. None enterococci and non-enterococci showed bile deconjugation and mucin degradation abilities, or other detrimental enzymatic activities.

Conclusions
To our knowledge, this is the first description of mef(A/E) in the genera Pediococcus and Weissella, and lnu(A) in the genus Pediococcus. The in vitro subtractive screening presented in this work constitutes a valuable strategy for the large-scale preliminary selection of putatively safe LAB intended for use as probiotics in aquaculture.
Clinical features and phylogenetic analysis of Coxsackievirus A9 in Northern Taiwan in 2011
Authors: Huang Yi-Chuan , Chu Ying-Hsia , Yen Ting-Yu , Huang Wen-Chan , Huang Li-Min , Cheng Ai-Ling , Wang Hurng-Yi , Chang Luan-Yin 
Published: January 2013

AbstractBackgroundCoxsackievirus A9 (CA9) was one of the most prevalent serotype of enteroviral infections in Taiwan in 2011. After several patient series were reported in the 1960s and 1970s, few studies have focused on the clinical manifestations of CA9 infections. Our study explores and deepens the current understanding of CA9.MethodsWe analyzed the clinical presentations of 100 culture-proven CA9-infected patients in 2011 by reviewing their medical records and depicted the CA9 phylogenetic tree.ResultsOf the 100 patients with culture-proven CA9 infections, the mean (SD) age was 4.6 (3.4) years and the male to female ratio was 1.9. For clinical manifestations, 96 patients (96%) had fever and the mean (SD) duration of fever was 5.9 (3.4) days. Sixty one patients (61%) developed a skin rash, and the predominant pattern was a generalized non-itchy maculopapular rash without vesicular changes. While most patients showed injected throat, oral ulcers were found in only 19 cases (19%), among whom, 6 were diagnosed as herpangina. Complicated cases included: aseptic meningitis (n=8), bronchopneumonia (n=6), acute cerebellitis (n=1), and polio-like syndrome (n=1). Phylogenetic analysis for current CA9 strains is closest to the CA9 isolate 27-YN-2008 from the border area of mainland China and Myanmar.ConclusionsThe most common feature of CA9 during the 2011 epidemic in Taiwan is generalized febrile exanthema rather than herpangina or hand, foot, and mouth disease. Given that prolonged fever and some complications are possible, caution should be advised in assessing patients as well as in predicting the clinical course.