bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a general‐purpose method for cell purification using Cre/loxP‐mediated recombination
Authors: Kuroki Shunsuke , Akiyoshi Mika , Ideguchi Ko , Kitano Satsuki , Miyachi Hitoshi , Hirose Michiko , Mise Nathan , Abe Kuniya , Ogura Atsuo , Tachibana Makoto 
Published: May 2015

SummaryA mammalian body is composed of more than 200 different types of cells. The purification of a certain cell type from tissues/organs enables a wide variety of studies. One popular cell purification method is immunological isolation, using antibodies against specific cell surface antigens. However, this is not a general‐purpose method, since suitable antigens have not been found in certain cell types, including embryonic gonadal somatic cells and Sertoli cells. To address this issue, we established a knock‐in mouse line, named R26 KI, designed to express the human cell surface antigen hCD271 through Cre/loxP‐mediated recombination. First, we used the R26 Kl mouse line to purify embryonic gonadal somatic cells. Gonadal somatic cells were purified from the R26 KI; Nr5a1‐Cre‐transgenic (tg) embryos almost equally as efficiently as from Nr5a1‐hCD271‐tg embryos. Second, we used the R26 KI mouse line to purify Sertoli cells successfully from R26 KI; Amh‐Cre‐tg testes. In summary, we propose that the R26 KI mouse line is a powerful tool for the purification of various cell types. genesis 53:387–393, 2015. © 2015 Wiley Periodicals, Inc.
Risk for self‐reported anorexia or bulimia nervosa based on drive for thinness and negative affect clusters/dimensions during adolescence: A three‐year prospective study of the TChAD cohort
Authors: Peñas‐Lledó Eva , Bulik Cynthia M. , Lichtenstein Paul , Larsson Henrik , Baker Jessica H. 
Published: May 2015

ABSTRACTObjectiveThis study explored the cross‐sectional and predictive effect of drive for thinness and/or negative affect scores on the development of self‐reported anorexia nervosa (AN) and bulimia nervosa (BN).MethodK‐means were used to cluster the Eating Disorder Inventory‐Drive for Thinness (DT) and Child Behavior Checklist Anxious/Depressed (A/D) scores from 615 unrelated female twins at age 16–17. Logistic regressions were used to assess the effect of these clusters on self‐reported eating disorder diagnosis at ages 16–17 (n = 565) and 19–20 (n = 451).ResultsDT and A/D scores were grouped into four clusters: Mild (scores lower than 90th percentile on both scales), DT (higher scores only on DT), A/D (higher scores only on A/D), and DT‐A/D (higher scores on both the DT and A/D scales). DT and DT‐A/D clusters at age 16–17 were associated cross‐sectionally with AN and both cross‐sectionally and longitudinally with BN. The DT‐A/D cluster had the highest prevalence of AN at follow‐up compared with all other clusters. Similarly, an interaction was observed between DT and A/D that predicted risk for AN.DiscussionHaving elevated DT and A/D scores may increase risk for eating disorder symptomatology above and beyond a high score on either alone. Findings suggest that cluster modeling based on DT and A/D may be useful to inform novel and useful intervention strategies for AN and BN in adolescents. © 2015 Wiley Periodicals, Inc. (Int J Eat Disord 2015; 48:692–699)
The 30‐day metric in acute heart failure revisited: data from IN‐HF Outcome, an Italian nationwide cardiology registry
Authors: Di Tano Giuseppe , De Maria Renata , Gonzini Lucio , Aspromonte Nadia , Di Lenarda Andrea , Feola Mauro , Marini Marco , Milli Massimo , Misuraca Gianfranco , Mortara Andrea , Oliva Fabrizio , Pulignano Giovanni , Russo Giulia , Senni Michele , Tavazzi Luigi ,   on the behalf of the IN‐HF Outcome Investigators
Published: May 2015

AimsUnplanned readmissions early after a discharge from acute heart failure hospitalization are common and have become a reimbursement benchmark and marker of hospital quality. However, the competing risk of short‐term post‐discharge mortality is substantial.Methods and resultsUsing data from the prospective, nationwide Registry IN‐HF Outcome, we analysed the incidence and predictors of 30‐day mortality or readmissions and associated days‐alive‐out‐of‐hospital (DAOH) in 1520 patients discharged alive after admission for acute heart failure. Within 30 days after discharge, 94 patients (6.2%) were readmitted (91% for cardiovascular causes; 60% recurrent heart failure) and 42 (2.8%) died, 10 of which occurred during readmission. Overall, 126 patients (8.3%) met the combined endpoint. By multivariable logistic regression, worsening chronic heart failure as clinical presentation [odds ratio (OR) 1.83, 95% confidence interval (CI) 1.21–2.77, P = 0.005), inotropes during admission (OR 2.19, 95% CI 1.40–3.43, P = 0.0006), length of stay (OR 1.02, 95% CI 1.01–1.04, P = 0.002) and renin–angiotensin system inhibitors at discharge (OR 0.52, 95%CI 0.35–0.77, P = 0.001) independently predicted 30‐day all‐cause mortality and/or readmission (c‐statistic = 0.695). Per cent 30‐day DAOH was lower in patients with in‐hospital inotrope use, no renin–angiotensin system inhibitors prescription at discharge, New York Heart Association III–IV class at discharge, and correlated inversely with length of stay and age.ConclusionA clinical and biohumoral profile consistent with chronic advanced heart failure and end‐organ damage identifies acute heart failure patients discharged home from cardiology units, who are at highest risk of early death and/or readmission. These findings have practical implications for tailoring specific follow‐up.
Recognition of an Explosive and Mutagenic Water Pollutant, 2,4,6‐Trinitrophenol, by Cost‐Effective Luminescent MOFs
Authors: Ghosh Pritam , Saha Sourav Kumar , Roychowdhury Additi , Banerjee Priyabrata 
Published: May 2015

AbstractTwo luminescent MOFs, Zn‐NDC and Cd‐NDC (NDC = 2,6‐naphthalenedicarboxylate), which are capable of sensing 2,4,6‐trinitrophenol (TNP) and similar explosives and mutagens, are reported. Of these two MOFs, Zn‐NDC shows better response in sensing nitroaromatics like TNP and 4‐nitrobenzoic acid (4‐NBA). Compared to Zn‐NDC, Cd‐NDC is more selective in the detection of explosive and pollutant nitroaromatics (epNACs). Cd‐NDC is a selective TNP sensor over several other tested epNACs: 2,6‐dinitrotoluene (2,6‐DNT), nitrobenzene (NB), 4‐NBA, 1,3‐dinitrobenzene (1,3‐DNB), 3,4‐dinitrotoluene (3,4‐DNT), and 2,4,6‐trinitrotoluene (TNT). For both d10‐NDC MOFs, TNP sensitivity is supported by fluorescence quenching. The experiments have been carried out with deionized water as well as various other environmental water specimens collected from several parts of West Bengal, India. Spectroscopic results are further supported by theoretical DFT calculations.
Synthesis of BINAM‐Based Chiral Di‐1,2,3‐triazolylidene Complexes and Application of the Di‐NHC RhI Catalyst in Enantioselective Hydrosilylation
Authors: Sluijter Soraya N. , Jongkind Lukas J. , Elsevier Cornelis J. 
Published: May 2015

AbstractDespite the prolific use of (di‐)NHC complexes in homogeneous catalysis, there are relatively few reports on their successful application in asymmetric transformations. In this work the atropisomeric binaphthyl backbone was combined with readily obtainable 1,2,3‐triazolylidenes to develop a strongly electron‐donating C2‐symmetric ligand. The ligand was efficiently synthesized in a three‐step procedure in an overall yield of 91 % starting from commercially available materials. Strategies for the synthesis of the corresponding di‐NHC silver(I), palladium(II), rhodium(I), and iridium(I) complexes have been developed. The rhodium(I) complex was employed in the catalytic asymmetric hydrosilylation of ketones, providing good conversions at catalyst loadings as low as 0.2 mol‐% and giving chiral inductions of up to 51 % ee.
Solution Speciation and Stability of Cobalt‐Polyoxometalate Water Oxidation Catalysts by X‐ray Scattering
Authors: Goberna‐Ferrón Sara , Soriano‐López Joaquín , Galán‐Mascarós José Ramón , Nyman May 
Published: May 2015

AbstractIn the last 5 years several cobalt‐polyoxometalates (Co‐POMs) have been reported as all‐inorganic water oxidation catalysts (WOCs) with enhanced stability, because oxidative ligand decomposition is not possible. However, while they are promising homogeneous WOCs, they have been highly debated with regard to their stability under turnover conditions. For this reason, these systems are interesting subjects for speciation and stability studies using X‐ray scattering techniques – not broadly used in the WOC field. Here we rigorously characterize the speciation of Co‐POMs under catalytically relevant conditions. Our studies reveal that: 1) the clusters remain largely intact during reaction with a chemical oxidant, especially in the absence of phosphate buffer; and 2) common phosphate buffers have the greatest effect on the degradation of the Co‐POMs under these conditions. This study of the structure in the aqueous state under various conditions provides invaluable information in the understanding of the solution behaviour of Co‐POMs and in the development of the next generation of WOCs.
Discrete Polymetallic Arrangements of AgI and CuI Ions Based on Multiple Bridging Phosphane Ligands and π–π Interactions
Authors: Attenberger Bianca , El Sayed Moussa Mehdi , Brietzke Thomas , Vreshch Volodimir , Holdt Hans‐Jürgen , Lescop Christophe , Scheer Manfred 
Published: May 2015

AbstractA simple and straightforward approach to new polymetallic AgI and CuI supramolecules is presented. The reaction of N,P,N,P,N ligand 2 with AgI ions affords a trimetallic complex bearing a triangular Ag3 core; metallophilic interactions are stabilized by ligands that display a multiple bridging coordination mode as 10‐electron donors. Heteroleptic polymetallic AgI and CuI complexes based on ligand 2 and the 1,12‐diazaperylene (dape) ligand are obtained by an alternative molecular organization of the polymetallic arrays compared to that in homoleptic complexes of ligand 2.
Pheromone Bouquet of the Dried Bean Beetle, Acanthoscelides obtectus (Col.: Chrysomelidae), Now Complete
Authors: Vuts József , Francke Wittko , Mori Kenji , Zarbin Paulo H. G. , Hooper Antony M. , Millar Jocelyn G. , Pickett John A. , Tóth Miklós , Chamberlain Keith , Caulfield John C. , Woodcock Christine M. , Tröger Armin G. , Csonka Éva Bálintné , Birkett Michael A. 
Published: May 2015

AbstractMale‐specific volatile components, released by the dried bean beetle, Acanthoscelides obtectus, were identified as methyl (E,R)‐2,4,5‐tetradecatrienoate, methyl (2E,4Z,7Z)‐2,4,7‐decatrienoate, methyl (2E,4Z)‐2,4‐decadienoate, octadecanal and the sesquiterpenes (3Z,6E)‐ and (3E,6E)‐α‐farnesene. In olfactometer bioassays, pure methyl (E,R)‐2,4,5‐tetradecatrienoate was only weakly attractive to unmated females. However, a blend of the six identified compounds released in physiologically relevant ratios and doses proved to be as active as headspace odours collected from live males.
N‐(1‐Oxy‐2‐picolyl)oxalamic Acid as an Efficient Ligand for Copper‐Catalyzed Amination of Aryl Iodides at Room Temperature
Authors: Wang Yongbin , Ling Jing , Zhang Yu , Zhang Ao , Yao Qizheng 
Published: May 2015

AbstractN‐(1‐Oxy‐pyridin‐2‐ylmethyl)oxalamic acid was identified as efficient ligand for CuI‐catalyzed amination of aryl halides at room temperature. In our catalytic system, N‐arylation of cyclic secondary amines, primary amines, amino acids, and ammonia proceeded with moderate to excellent yields and high functional group tolerance.
Synthesis of 5‐Fluorotriazoles by Silver‐Mediated Fluorination of 5‐Iodotriazoles
Authors: Wang Damin , Sun Wenjing , Chu Taiwei 
Published: May 2015

AbstractA simple method was developed for the direct fluorination of 5‐iodotriazoles with AgF as fluoride source and N1,N1,N2,N2‐tetramethylethane‐1,2‐diamine (TMEDA) as ligand. This method is compatible with various functional groups. 5‐Iodotriazoles without aromatic groups at the 4‐position or aliphatic substituents at the N1 position can also be smoothly converted to the corresponding 5‐fluorotriazoles. Preliminary mechanism studies were also performed.
Synthesis and Biological Evaluation of Dichlorinated Chondramide Derivatives
Authors: Becker Dominic , Kazmaier Uli 
Published: May 2015

AbstractStraightforward synthetic protocols for the synthesis of new ethyl‐substituted dichlorinated chondramides with different methyl‐substitution patterns in the polyketide fragment have been developed. The methyl groups at the ϵ‐position can be removed completely without a significant influence on the biological activity. In contrast, after removal of the α‐methyl group, a significant drop in activity is observed. This is also observed if the configuration of the α‐methyl group is inverted.
Iron‐Catalysed Oxidative Ugi‐Type Multicomponent Reaction Using (Arylmethyl)amines as Imine Precursors
Authors: Dighe Shashikant U. , Kolle Shivalinga , Batra Sanjay 
Published: May 2015

AbstractAn efficient iron‐catalysed Ugi‐type three‐component reaction involving oxidation of (arylmethyl)amines to imines directly under air as a safe oxidant is reported. The reaction is executed as a one‐step procedure, and has a broad substrate scope with respect to all components.
Calibration of the Dutch‐Flemish PROMIS Pain Behavior item bank in patients with chronic pain
Authors: Crins M.H.P. , Roorda L.D. , Smits N. , de Vet H.C.W. , Westhovens R. , Cella D. , Cook K.F. , Revicki D. , van Leeuwen J. , Boers M. , Dekker J. , Terwee C.B. 
Published: May 2015

AbstractBackgroundThe aims of the current study were to calibrate the item parameters of the Dutch‐Flemish PROMIS Pain Behavior item bank using a sample of Dutch patients with chronic pain and to evaluate cross‐cultural validity between the Dutch‐Flemish and the US PROMIS Pain Behavior item banks. Furthermore, reliability and construct validity of the Dutch‐Flemish PROMIS Pain Behavior item bank were evaluated.MethodsThe 39 items in the bank were completed by 1042 Dutch patients with chronic pain. To evaluate unidimensionality, a one‐factor confirmatory factor analysis (CFA) was performed. A graded response model (GRM) was used to calibrate the items. To evaluate cross‐cultural validity, Differential item functioning (DIF) for language (Dutch vs. English) was evaluated. Reliability of the item bank was also examined and construct validity was studied using several legacy instruments, e.g. the Roland Morris Disability Questionnaire.ResultsCFA supported the unidimensionality of the Dutch‐Flemish PROMIS Pain Behavior item bank (CFI = 0.960, TLI = 0.958), the data also fit the GRM, and demonstrated good coverage across the pain behavior construct (threshold parameters range: −3.42 to 3.54). Analysis showed good cross‐cultural validity (only six DIF items), reliability (Cronbach's α = 0.95) and construct validity (all correlations ≥0.53).ConclusionsThe Dutch‐Flemish PROMIS Pain Behavior item bank was found to have good cross‐cultural validity, reliability and construct validity. The development of the Dutch‐Flemish PROMIS Pain Behavior item bank will serve as the basis for Dutch‐Flemish PROMIS short forms and computer adaptive testing (CAT).
Inflammatory pain memory facilitates occlusal interference‐induced masticatory muscle hyperalgesia in rats
Authors: Ding T‐T. , Xu X‐X. , Cao Ye , Liu C‐R. , Gan Y‐H. , Xie Q‐F. 
Published: May 2015

AbstractBackgroundPatients with an orofacial pain history appear to be more susceptible to occlusal interference pain in dental practice for unknown reasons. Pain memory has a critical function in subsequent pain perception. This study aims to explore whether orofacial pain memory could affect the masticatory muscle pain perception for occlusal interference.MethodsCross‐injection of 2% carrageenan into bilateral masseters in male rats was carried out to establish the inflammatory pain memory model. The effects of pain memory on masseter muscle nociception were tested by applying crowns with heights beyond the occlusal plane by 0.2 or 0.4 mm onto a maxillary molar 2 weeks after inflammation in the right masseter. The 0.4‐mm crowns were removed on day 2 or day 4 after application to further confirm the effects of pain memory. Moreover, memory impairment was established using ibotenic acid (IBO) infusion into the bilateral hippocampus, followed by behaviour tests, including the Morris water maze test and the locomotor activity test. The relationship between pain memory and occlusal interference‐induced masseter muscle pain perception was subsequently re‐examined. The head withdrawal thresholds of masseters on both sides were measured to reflect the perception.ResultsInflammatory pain memory aggravated the 0.2‐mm crown‐induced mechanical hyperalgesia of the masseters, but not in the 0.4‐mm crown group. However, the recovery of the 0.4‐mm crown‐induced mechanical hyperalgesia was postponed. The effects of pain memory were reversed in rats with impaired mnemonic function of the hippocampus.ConclusionsInflammatory pain memory facilitated occlusal interference‐induced masseter muscle pain.
Mangrove‐forest evolution in a sediment‐rich estuarine system: opportunists or agents of geomorphic change?
Authors: Swales Andrew , Bentley Samuel J. , Lovelock Catherine E. 
Published: May 2015

AbstractThe majority of the world's mangrove forests occur on mostly mineral sediments of fluvial origin. Two perspectives exist on the biogeomorphic development of these forests, i.e. that mangroves are opportunistic, with forest development primarily driven by physical processes, or alternatively that biophysical feedbacks strongly influence sedimentation and resulting geomorphology. On the Firth of Thames coast, New Zealand, we evaluate these two possible scenarios for sediment accumulation and forest development using high‐resolution sedimentary records and a detailed chronology of mangrove‐forest (Avicennia marina) development since the 1950s. Cores were collected along a shore‐normal transect of known elevation relative to mean sea level (MSL). Activities for lead‐210 (210Pb), caesium‐137 (137Cs) and beryllium‐7 (7Be), and sediment properties were analysed, with 210Pb sediment accumulation rates (SARs), compensated for deep subsidence (~8 mm yr−1) used as a proxy for elevation gain. At least four phases of forest development since the 1950s are recognized. An old‐growth forest developed by the late‐1970s with more recent seaward forest expansion thereafter. Excess 210Pb profiles from the old‐growth forest exhibit relatively low SARs near the top (7–12 mm yr−1) and bottom (10–22 mm yr−1) of cores, separated by an interval of higher SARs (33–100 mm yr−1). A general trend of increasing SAR over time characterizes the recent forest. Biogeomorphic evolution of the system is more complex than simple mudflat accretion/progradation and mangrove‐forest expansion. Surface‐elevation gain in the old‐growth forest displays an asymptotic trajectory, with a secondary depocentre developing on the seaward mudflat from the mid‐1970s. Two‐ to ten‐fold increases in 210Pb SARs are unambiguously large and occurred years to decades before seedling recruitment, demonstrating that mangroves do not measurably enhance sedimentation over annual to decadal timescales. This suggests that mangrove‐forest development is largely dependent on physical processes, with forests occupying mudflats once they reach a suitable elevation in the intertidal. Copyright © 2015 John Wiley & Sons, Ltd.
Synthesis of Novel Substituted Indazoles via Nucleophilic Substitution of Hydrogen (SNH)
Authors: Kouakou Assoman , Abbassi Najat , Chicha Hakima , Ammari Lahcen El , Saadi Mohamed , Rakib El Mostapha 
Published: May 2015

ABSTRACTNew four‐substituted indazoles 4a–e were synthesized by regioselective nucleophilic substitution of hydrogen of N‐alkyl‐7‐nitroindazoles 2a,b with arylacetonitriles 3a–c. Compounds 4a–e were reacted with arylsulfonyl chloride in pyridine to give some new indazole linked sulfonamides with good yields. The SNH at position C‐4 of 7‐nitroindazole with arylacetonitrile is confirmed by X‐ray diffraction analysis of compounds 4e and 6a.
Methyl and Dimethyl o‐Xylenyl‐Substituted Fullerene Acceptors for Polymer Solar Cells
Authors: Kim Hee Un , Park Jong Baek , Grimsdale Andrew C. , Hwang Do‐Hoon 
Published: May 2015

AbstractA series of fullerene derivatives based on methyl‐ and dimethyl‐substituted o‐xylenyl groups were synthesized via DielsAlder reaction from fullerene (C60) and dienes derived from α,α′‐dibromo‐o‐xylene derivatives. The synthesized 1‐methyl‐4,5‐xylenyl‐ and 1,2‐dimethyl‐4,5‐xylenyl‐substituted fullerene mono‐, bis‐, and trisadducts, i.e., M_OXCMA, M_OXCBA, M_OXCTA, DM_OXCMA, DM_OXCBA, and DM_OXCTA, were used as electron‐acceptor materials for polymer solar cells and have good solubility in common organic solvents. The SternVolmer quenching constants for poly(3‐hexylthiophene) (P3HT) as a fluorophore with M_OXCMA, M_OXCBA, M_OXCTA, DM_OXCMA, DM_OXCBA, and DM_OXCTA as quenchers were 1.35×104, 7.67×103, 3.72×103, 1.38×104, 7.95×103, and 4.10×103 M−1, respectively. These values correlate with the binding affinities of the fluorophore and quencher. The lowest unoccupied molecular orbital energy levels increased with increasing number of adducts on the fullerene because of their decreasing number of sp2‐hybridized carbons. Photovoltaic devices were fabricated using P3HT and the mono‐, bis‐, and trisadducts as electron donors and acceptors, respectively. Among these, the cells with an ITO/PEDOT : PSS/P3HT : DM_OXCBA/LiF/Al structure had the highest power conversion efficiency of 4.77 % with an open‐circuit voltage of 0.88 V, a short‐circuit current density of 10.52 mA cm−2, and a fill factor of 0.51 under AM 1.5G (100 mW cm−2) illumination.
Trends in Media Reports, Oral Bisphosphonate Prescriptions, and Hip Fractures 1996–2012: An Ecological Analysis
Authors: Jha Smita , Wang Zhong , Laucis Nicholas , Bhattacharyya Timothy 
Published: May 2015

ABSTRACT

Bisphosphonates are effective for the treatment of osteoporosis despite recent reports of safety concerns such as atypical femur fracture. We conducted an ecological analysis of relevant media reports, oral bisphosphonate use, and fracture outcomes in the United States. Trends in media reports and public interest of bisphosphonates were quantified using data from Google Trends. Data from the Medical Expenditure Panel Survey (MEPS) and the National Inpatient Sample (NIS) were used to estimate the trends in oral bisphosphonate use among patients aged 55 years and older and hospitalizations for intertrochanteric and subtrochanteric fractures, respectively. These trends in the prevalence of oral bisphosphonate use and the age-adjusted incidence rate of intertrochanteric and subtrochanteric fractures were examined from 1996 to 2012. A series of spikes in Internet search activity for alendronate (Fosamax) occurred between 2006 and 2010 immediately following media reports of safety concerns. Oral bisphosphonate use declined by greater than 50% between 2008 and 2012 (p &lt; 0.001) after increasing use for more than a decade. The decline was more common in patients with lower education levels. Intertrochanteric hip fractures declined from 1996 through 2006 (p &lt; 0.001) and continued to decline from 2008 to 2012 (p &lt; 0.05). Subtrochanteric and diaphyseal fractures showed a steady and significant increase from 2002 to 2011 (p &lt; 0.05). However, the incidence decreased from a peak of 30.5 per 100,000 in 2011 to 26.7 per 100,000 in 2012. The plateauing and subsequent decline in oral bisphosphonate use since 2006 coincided with reports of safety concerns of bisphosphonates, despite the fact that U.S. Food and Drug Administration (FDA) and American Society of Bone and Mineral Research (ASBMR) reports did not recommend any safety restrictions on their use. This decline in oral bisphosphonate use was followed by the decline in the incidence of subtrochanteric and diaphyseal fractures. © 2015 American Society for Bone and Mineral Research.