bluuebunny/crossref_metadata_2025_split huggingface dataset:
Psychiatric morbidity across the life course and provoked vulvodynia: is it dependent upon the presence of non–stress-related immune dysfunction?
Authors: Harlow Bernard L , Mühlrad Hanna , Yan Jane , Linnros Evelina , Lu Donghao , Fox Matthew P , Bohm-Starke Nina 
Published: July 2024

Abstract

Background
Vulvodynia impacts up to 8% of women by age 40, and these women may have a more compromised immune system than women with no vulvar pain history.

Aim
Given that psychiatric morbidity is associated with vulvodynia and is known to activate immune inflammatory pathways in the brain and systemically, we sought to determine whether the association between psychiatric morbidity and vulvar pain was independent of or dependent upon the presence of immune-related conditions.

Methods
Women born in Sweden between 1973 and 1996 with localized provoked vulvodynia (N76.3) and/or vaginismus (N94.2 or F52.5) diagnosed between 2001 and 2018 were matched to two women from the same birth year with no vulvar pain. International Statistical Classification of Diseases and Related Health Problems (ICD-9 or -10 codes) were used to identify women with a history of depression, anxiety, attempted suicide, neurotic disorders, stress-related disorders, behavioral syndromes, personality disorders, psychotic disorders, or chemical dependencies, as well as a spectrum of immune-related conditions. The Swedish National Prescribed Drug Register was used to identify women with filled prescriptions of antidepressants or anxiolytics.

Outcomes
Vulvodynia, vaginismus, or both were outcomes assessed in relation to psychiatric morbidity.

Results
Women with vulvodynia, vaginismus, or both, relative to those without vulvar pain, had adjusted odds ratios between 1.4 and 2.3, with CIs highly compatible with harmful effects. When we assessed women with and those without a lifetime history of immune-related conditions separately, we also observed elevated odds ratios in both groups for mood, anxiety, and neurotic and stress disorders.

Clinical implications
Documenting psychiatric impairment as a cause or consequence of vulvodynia is critical in clinical practice because psychiatric conditions may impact treatment efficacy.

Strengths and Limitations
Strengths of this study include a data source that represents the entire population of women in Sweden that is known to be highly accurate because Sweden provides universal healthcare. Limitations include difficulty in making an accurate assessment of temporality between psychiatric morbidity and the first onset of vulvar pain. In addition, because Swedish registry data have limited information on lifestyle, behavioral, and anthropomorphic factors such as smoking, diet, physical activity, and obesity, these conditions could not be assessed as confounders of psychiatric morbidity and vulvar pain.

Conclusions
Immune pathways by which women with psychiatric conditions increase their risk of vulvar pain could be independent from other immune pathways.
Pathways to sustainable development: an overview of the progress and obstacles of hydroelectric power production in Angola
Authors: Joaquim Francisco Domingos , Lacerda Cordeiro Mikael Jhordan , de Abreu Pereira Uhr Daniel , Gallego Ziero Uhr Júlia 
Published: July 2024

Abstract
This article offers an in-depth examination of the historical trajectory of Angola’s hydroelectric energy sector, contextualizing its evolution within the country’s political and economic landscape. It begins by tracing the origins of hydroelectric development. Subsequently, it delves into the post-independence era marked by political turmoil and civil conflict, elucidating the challenges faced by the energy infrastructure amidst the ravages of war. Moreover, the article scrutinizes Angola’s post-conflict reconstruction efforts, particularly in revitalizing hydroelectric facilities and formulating strategic energy policies. It assesses the regulatory framework governing the energy sector, identifying deficiencies and proposing avenues for enhancement to ensure transparency and efficiency. This study explores the prospects and challenges associated with the Baynes Binational Hydroelectric Project on the Angola–Namibia border, highlighting its potential to foster regional cooperation and bolster energy security. The article underscores institutional reforms and strategic investments to propel Angola towards a more resilient and inclusive energy future.
Our Growing Volume Electron Microscopy Community
Authors: Czymmek Kirk J , Darrow Michele C , Verkade Paul , Ortega-Sandoval Mario E , Wacker Kate 
Published: July 2024

Abstract
Volume electron microscopy (vEM) represents a powerful group of electron microscopy workflows that enable collection of large three-dimensional volumes at ultrastructural resolution to interrogate cells and tissues across length scales. Due to the breadth of approaches, users are challenged with numerous specialized and nuanced workflows and the requirements for handling, segmenting, visualizing, and quantifying large and complex datasets. As such, a vEM community of core facilities, early adopters, and commercial vendors of vEM solutions have come together to reduce the barriers for successful adoption of vEM. Supported by the Chan Zuckerberg Initiative, the rapidly expanding community has built a broad coalition sharing knowledge and working together to accelerate improvements in results, speed, and automation in vEM workflows. Here we provide an overview of the vEM concept, our growing resources, and an open invitation to join our community and upcoming vEM Technology Forum with technical talks, discussions, hands-on demonstrations, and networking.
Insights Into the Role of Porosity in Lithium-Ion Battery Electrode Performance Using the TESCAN Unitom HR Micro-CT
Authors: Boone Marijn 
Published: July 2024

Abstract
Use of lithium-ion batteries is extensive with many applications. One of the most common analyses performed in quality control for battery production is the characterization of porosity after calendaring. In this study we report the use of micro-CT to evaluate porosity at the micron scale to enable geometrical quantification of the copper anode current collector. Micro-CT resolution permitted analysis of particle disintegration and delamination from the current collector, which provided a starting point for porosity characterization. Segmentation analysis revealed an average porosity below 30% with a wide range of pore sizes from 1.2 µm to 9.6 µm. This information is critical for the optimization of electrical resistance and may improve the economy of production by reducing the time for battery cell soaking with electrolyte.
Structural basis for a highly conserved RNA-mediated enteroviral genome replication
Authors: Das Naba Krishna , Vogt Jeff , Patel Alisha , Banna Hasan Al , Koirala Deepak 
Published: July 2024

Abstract
Enteroviruses contain conserved RNA structures at the extreme 5′ end of their genomes that recruit essential proteins 3CD and PCBP2 to promote genome replication. However, the high-resolution structures and mechanisms of these replication-linked RNAs (REPLRs) are limited. Here, we determined the crystal structures of the coxsackievirus B3 and rhinoviruses B14 and C15 REPLRs at 1.54, 2.2 and 2.54 Å resolution, revealing a highly conserved H-type four-way junction fold with co-axially stacked sA-sD and sB-sC helices that are stabilized by a long-range A•C•U base-triple. Such conserved features observed in the crystal structures also allowed us to predict the models of several other enteroviral REPLRs using homology modeling, which generated models almost identical to the experimentally determined structures. Moreover, our structure-guided binding studies with recombinantly purified full-length human PCBP2 showed that two previously proposed binding sites, the sB-loop and 3′ spacer, reside proximally and bind a single PCBP2. Additionally, the DNA oligos complementary to the 3′ spacer, the high-affinity PCBP2 binding site, abrogated its interactions with enteroviral REPLRs, suggesting the critical roles of this single-stranded region in recruiting PCBP2 for enteroviral genome replication and illuminating the promising prospects of developing therapeutics against enteroviral infections targeting this replication platform.
The human mitochondrial translation factor TACO1 alleviates mitoribosome stalling at polyproline stretches
Authors: Brischigliaro Michele , Krüger Annika , Moran J Conor , Antonicka Hana , Ahn Ahram , Shoubridge Eric A , Rorbach Joanna , Barrientos Antoni 
Published: July 2024

Abstract
The prokaryotic translation elongation factor P (EF-P) and the eukaryotic/archaeal counterparts eIF5A/aIF5A are proteins that serve a crucial role in mitigating ribosomal stalling during the translation of specific sequences, notably those containing consecutive proline residues (1,2). Although mitochondrial DNA-encoded proteins synthesized by mitochondrial ribosomes also contain polyproline stretches, an EF-P/eIF5A mitochondrial counterpart remains unidentified. Here, we show that the missing factor is TACO1, a protein causative of a juvenile form of neurodegenerative Leigh's syndrome associated with cytochrome c oxidase deficiency, until now believed to be a translational activator of COX1 mRNA. By using a combination of metabolic labeling, puromycin release and mitoribosome profiling experiments, we show that TACO1 is required for the rapid synthesis of the polyproline-rich COX1 and COX3 cytochrome c oxidase subunits, while its requirement is negligible for other mitochondrial DNA-encoded proteins. In agreement with a role in translation efficiency regulation, we show that TACO1 cooperates with the N-terminal extension of the large ribosomal subunit bL27m to provide stability to the peptidyl-transferase center during elongation. This study illuminates the translation elongation dynamics within human mitochondria, a TACO1-mediated biological mechanism in place to mitigate mitoribosome stalling at polyproline stretches during protein synthesis, and the pathological implications of its malfunction.
Transfer of Respiratory Syncytial Virus Prefusion F Protein Antibody in Low Birthweight Infants
Authors: Kachikis Alisa B , Rumfelt Kalee , Pike Mindy , Sosa Monica , Stolarczuk Jennifer E , Cho Hye , Eckert Linda O , Martin Emily T , Englund Janet A 
Published: July 2024

Abstract

Background
Respiratory syncytial virus (RSV)–associated lower respiratory tract infection contributes significantly to morbidity/mortality worldwide in low birthweight (LBW) infants (&lt;2500 g). Studies have demonstrated decreased maternal immunoglobulin G (IgG) transfer of various antibodies to LBW infants. We aimed to evaluate naturally acquired RSV anti–prefusion F protein (anti-preF) antibody transfer in pregnancies with LBW versus normal birthweight (NBW) infants.

Methods
In this cohort study conducted among pregnant individuals and their infants, we tested paired maternal and singleton infant cord samples for RSV anti-preF IgG via an electrochemiluminescence immunoassay, using linear regression to evaluate associations between LBW and anti-preF IgG. Covariates included seasonality, insurance, small-for-gestational-age birthweight, and gestational age at delivery.

Results
We tested maternal/cord RSV anti-preF IgG from 54 and 110 pregnancies with LBW and NBW infants, respectively. Of LBW infants, 22 (40.7%) were born both preterm and with small-for-gestational-age birthweight. The median (interquartile range) gestational age at delivery and birthweight were 34.0 (31.7–37.1) weeks and 1902 (1393–2276) g for LBW infants versus 39.1 (38.3–39.9) weeks and 3323 (3109–3565) g for NBW infants (both P &lt; .001). In unadjusted comparisons, preterm infants had significantly lower cord anti-preF IgG levels and cord-maternal IgG ratios compared with full-term infants, while LBW infants had significantly lower cord-maternal IgG ratios than NBW infants (all P &lt; .01). After adjustment for covariates, there was no difference in cord-maternal IgG ratios (β =−0.29 [95% confidence interval, −.63 to .05]) between LBW and NBW infants.

Conclusions
We documented robust transfer of maternal RSV anti-preF IgG in pregnancies with both LBW and NBW infants. Further studies are needed to assess immune protection in at-risk infants.
Epidemiology, Clinical Manifestations, Treatment, and Outcome of Mucormycosis: A Review of 77 Cases From a Single Center in France
Authors: Denis Blandine , Resche-Rigon Matthieu , Raffoux Emmanuel , Ronchetti Anne-Marie , Dudoignon Emmanuel , Verillaud Benjamin , Valade Sandrine , Lorillon Gwenaël , Rabian Florence , Xhaard Aliénor , Touratier Sophie , Hamane Samia , Alanio Alexandre , De Castro Nathalie 
Published: July 2024

Abstract

Background
The aim of this study was to assess the epidemiology, clinical manifestations, and outcome of mucormycosis over 15 years in a single center in France.

Methods
We conducted a retrospective analysis of all mucormycosis cases in our institution from 1 January 2006 to 31 December 2020 and analyzed patients’ medical records, laboratory results, and treatment to describe the epidemiology, clinical manifestations, diagnosis, treatment, and outcome. Mucorales quantitative polymerase chain reaction (qPCR) for the diagnosis was implemented in 2015.

Results
Seventy-seven mucormycosis cases were analyzed in 77 patients, with a median age of 54 years (60% male). Identified risk factors were hematological diseases (46 cases [60%]), solid malignancies (2 cases), solid organ transplants (3), burns (18), diabetes only (7), and trauma (1). Sites of infection were lungs (42%), sinus (36%), skin (31%), central nervous system (9%), liver (8%), others (6%), and disseminated (12%). Diagnosis remained difficult and qPCR contributed to mucormycosis diagnosis in 30% of cases. Among hematology patients, serum qPCR was the only positive test in 15% of cases. A mixed mold infection was diagnosed in 24 of 77 (31%) patients. Surgical treatment was undertaken in 43 (56%) cases. Most patients received liposomal amphotericin B (89%), with a combination therapy in 18 of 77 cases (23%). Three-month survival rate was 40% (95% confidence interval [CI], .30–.53]). As for treatment, adjunction of surgery (hazard ratio, 0.47 [95%CI, .25–.91); P = 0.02) was associated with lower mortality.

Conclusions
Mucormycosis remained associated with high mortality, especially in the hematological and burn populations. Surgery in combination with antifungal treatment was associated with improved survival.
PARP inhibitor therapy in patients with IDH1 mutated cholangiocarcinoma
Authors: Mohan Arathi , Quingalahua Elit , Gunchick Valerie , Paul Simi , Kumar-Sinha Chandan , Crysler Oxana , Zalupski Mark M , Sahai Vaibhav 
Published: July 2024

Abstract

Background
Isocitrate dehydrogenase 1 (IDH1) missense mutations occur at a frequency of 10%-15% in intrahepatic cholangiocarcinoma (iCCA). IDH1 mutations result in accumulation of (R)-2-hydroxyglutarate, an oncometabolite that leads to DNA hypermethylation and impairment of homologous recombination (HR). Impairment of HR results in a “BRCAness” phenotype which may confer sensitivity to poly(ADP ribose) polymerase (PARP) inhibition.

Methods
We conducted a retrospective cohort review to identify patients with advanced, IDH1 mutated iCCA treated with a PARP inhibitor (PARPi) at the University of Michigan between 2018 and 2023. Patients are described with respect to prior lines of therapy, response to platinum-based chemotherapy, and progression-free survival (PFS) and overall survival (OS) from the time of PARPi initiation.

Results
Between 2018 and 2023 we identified 40 patients with IDH1 mutated iCCA of which 6 patients were treated with a PARPi as monotherapy or in combination with an ATR inhibitor or anti-PD-1 immune checkpoint inhibitor. Majority of patients (n = 5) carried an IDH1 R132C mutation per tissue-based next generation sequencing. All patients had previously received at least one line of cisplatin-based systemic therapy for advanced disease prior to treatment with PARPi. PFS and OS from time of PARPi initiation ranged from 1.4 to 18.5 months and 2.8 to 42.4 months, respectively. Best response on PARPi therapy included 2 partial responses.

Conclusion
This is the first case series to describe PARPi treatment in IDH1 mutated iCCA. Results underscore the limitation of PARPi monotherapy, potentially support combined PARPi therapies, and highlight a need for effective treatment options for patients with IDH1 mutated iCCA.
Spatial immunophenotyping using multiplexed imaging of immune follicles in secondary lymphoid tissues
Authors: Allam Mayar , Hu Thomas , Fang Zhou , Pi Michelle , Singh Ankur , Coskun Ahmet F 
Published: July 2024

Abstract
Secondary lymphoid organs (SLOs), including tonsils (TS), lymph nodes (LN), and Peyer's Patches, exhibit complementary immune functions. However, little is known about the spatial organization of immune cells and extracellular matrix (ECM) in the SLOs. Traditional imaging is limited to a few markers, confining our understanding of the differences between the SLOs. Herein, imaging mass cytometry addressed this gap by simultaneously profiling 25-plex proteins in SLO tissues at subcellular resolution. The antibody panel targeted immune, stromal, chemokine, epigenetic, and functional markers. For robust cell identification, a computational workflow SpatialVizPheno was developed to spatially phenotype 999,970 cells using two approaches, including manual gating and semi-supervised gating, iterative clustering, and annotation. LN exhibited the highest density of B cells while the intestinal tissues contained the highest proportion of regulatory and follicular helper T cells. SpatialVizPheno identified the most prevalent interaction between follicular dendritic cells and stromal cells (SCs), plasmablasts/plasma cells, and the SCs across the lymphoid tissues. Collagen-enriched regions were associated with the spatial orientation of B cell follicles in both TS and LN tissues, but not in intestinal lymphoid tissues. Such spatial differences of immunophenotypes and ECM in different SLO tissues can be used to quantify the relationship between cellular organization and ultimate immune responses.
Measuring the Extent to Which Voter Fraud Beliefs Link Election Reforms to Voter Confidence in the United States
Authors: Coll Joseph A 
Published: July 2024

Abstract
Proponents of restrictive voting policies argue that they are necessary to reduce fraud beliefs and instill confidence in elections. However, recent scholarship examining this dynamic in the United States finds that the effects of voting restrictions on fraud beliefs and voter confidence differ by party, suggesting the extent to which fraud beliefs undergird this relationship may also differ by party. Whether voter fraud beliefs tie voter restrictions to voter confidence is inherently important given the assumed role of voter fraud beliefs and voter restrictions in increasing voter confidence, an issue well highlighted in the 2020 election. This study contributes to this literature by measuring the extent to which voter fraud beliefs undergird the relationship between restrictive election policies and voter confidence among Republicans and Democrats. Findings suggest that voter fraud beliefs play a significant and substantive mediating role for Republicans but not Democrats, in line with prominent theories arguing that partisan elite signaling colors electoral reform perceptions.
An Introduction to the Human Connectome Project for Early Psychosis
Authors: Jacobs Grace R , Coleman Michael J , Lewandowski Kathryn E , Pasternak Ofer , Cetin-Karayumak Suheyla , Mesholam-Gately Raquelle I , Wojcik Joanne , Kennedy Leda , Knyazhanskaya Evdokiya , Reid Benjamin , Swago Sophia , Lyons Monica G , Rizzoni Elizabeth , John Omar , Carrington Holly , Kim Nicholas , Kotler Elana , Veale Simone , Haidar Anastasia , Prunier Nicholas , Haaf Moritz , Levitt James J , Seitz-Holland Johanna , Rathi Yogesh , Kubicki Marek , Keshavan Matcheri S , Holt Daphne J , Seidman Larry J , Öngür Dost , Breier Alan , Bouix Sylvain , Shenton Martha E 
Published: July 2024

Abstract

Background
The time following a recent onset of psychosis is a critical period during which intervention may be maximally effective. Studying individuals in this period also offers an opportunity to investigate putative brain biomarkers of illness prior to the long-term effects of chronicity and medication. The Human Connectome Project for Early Psychosis (HCP-EP) was funded by the National Institutes of Mental Health (NIMH) as an extension of the original Human Connectome Project’s approach to understanding the human brain and its structural and functional connections.

Design
The HCP-EP data were collected at 3 sites in Massachusetts (Beth Israel Deaconess Medical Center, McLean Hospital, and Massachusetts General Hospital), and one site in Indiana (Indiana University). Brigham and Women’s Hospital served as the data coordination center and as an imaging site.

Results
The HCP-EP dataset includes high-quality clinical, cognitive, functional, neuroimaging, and blood specimen data acquired from 303 individuals between the ages of 16–35 years old with affective psychosis (n = 75), non-affective psychosis (n = 148), and healthy controls (n = 80). Participants with early psychosis were within 5 years of illness onset (mean duration = 1.9 years, standard deviation = 1.4 years). All data and novel or modified analytic tools developed as part of the study are publicly available to the research community through the NIMH Data Archive (NDA) or GitHub (https://github.com/pnlbwh).

Conclusions
This paper provides an overview of the specific HCP-EP procedures, assessments, and protocols, as well as a brief characterization of the study participants to make it easier for researchers to use this rich dataset. Although we focus here on discussing and comparing affective and non-affective psychosis groups, the HCP-EP dataset also provides sufficient information for investigators to group participants differently.
Exploring nicotinamide adenine dinucleotide precursors across biosynthesis pathways: Unraveling their role in the ovary
Authors: Ren Caifang , Zhang Shuang , Chen Yanyan , Deng Kaiping , Kuang Meiqian , Gong Zihao , Zhang Ke , Wang Panqi , Huang Pan , Zhou Zhengrong , Gong Aihua 
Published: July 2024

AbstractNatural Nicotinamide Adenine Dinucleotide (NAD+) precursors have attracted much attention due to their positive effects in promoting ovarian health. However, their target tissue, synthesis efficiency, advantages, and disadvantages are still unclear. This review summarizes the distribution of NAD+ at the tissue, cellular and subcellular levels, discusses its biosynthetic pathways and the latest findings in ovary, include: (1) NAD+ plays distinct roles both intracellularly and extracellularly, adapting its distribution in response to requirements. (2) Different precursors differs in target tissues, synthetic efficiency, biological utilization, and adverse effects. Importantly: tryptophan is primarily utilized in the liver and kidneys, posing metabolic risks in excess; nicotinamide (NAM) is indispensable for maintaining NAD+ levels; nicotinic acid (NA) constructs a crucial bridge between intestinal microbiota and the host with diverse functions; nicotinamide riboside (NR) and nicotinamide mononucleotide (NMN) increase NAD+ systemically and can be influenced by delivery route, tissue specificity, and transport efficiency. (3) The biosynthetic pathways of NAD+ are intricately intertwined. They provide multiple sources and techniques for NAD+ synthesis, thereby reducing the dependence on a single molecule to maintain cellular NAD+ levels. However, an excess of a specific precursor potentially influencing other pathways. In addition, Protein expression analysis suggest that ovarian tissues may preferentially utilize NAM and NMN. These findings summarize the specific roles and potential of NAD+ precursors in enhancing ovarian health. Future research should delve into the molecular mechanisms and intervention strategies of different precursors, aiming to achieve personalized prevention or treatment of ovarian diseases, and reveal their clinical application value.