bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of experiments reveals critical parameters for pilot‐scale freeze‐and‐thaw processing of L‐lactic dehydrogenase
Authors: Roessl Ulrich , Humi Sebastian , Leitgeb Stefan , Nidetzky Bernd 
Published: March 2015

AbstractFreezing constitutes an important unit operation of biotechnological protein production. Effects of freeze‐and‐thaw (F/T) process parameters on stability and other quality attributes of the protein product are usually not well understood. Here a design of experiments (DoE) approach was used to characterize the F/T behavior of L‐lactic dehydrogenase (LDH) in a 700‐mL pilot‐scale freeze container equipped with internal temperature and pH probes. In 24‐hour experiments, target temperature between –10 and –38°C most strongly affected LDH stability whereby enzyme activity was retained best at the highest temperature of –10°C. Cooling profile and liquid fill volume also had significant effects on LDH stability and affected the protein aggregation significantly. Parameters of the thawing phase had a comparably small effect on LDH stability. Experiments in which the standard sodium phosphate buffer was exchanged by Tris‐HCl and the non‐ionic surfactant Tween 80 was added to the protein solution showed that pH shift during freezing and protein surface exposure were the main factors responsible for LDH instability at the lower freeze temperatures. Collectively, evidence is presented that supports the use of DoE‐based systematic analysis at pilot scale in the identification of F/T process parameters critical for protein stability and in the development of suitable process control strategies.
Engineering a Bacterial Tape Recorder
Authors: Prokup Alexander , Deiters Alexander 
Published: March 2015

AbstractA method has been developed to produce and integrate single‐stranded DNA into genomic locations in bacteria in response to exogenous signals. The system functions similarly to a cellular tape recorder by writing information into DNA and reading it at a later time. Much like other cellular memory platforms, its operation is based on DNA recombinase function. However, the scalability and recording capacity have been improved over previous designs. In addition, memory storage was reversible and could be recorded in response to analogue inputs, such as light exposure. This modular memory writing system is an important addition to the genomic editing toolbox available for synthetic biology.
The matricellular protein CCN1 regulates TNF‐α induced vascular endothelial cell apoptosis
Authors: Zhang Jin , Wu Gongxiong , Dai Haibin 
Published: March 2015

AbstractDue to the epidemic obesity and associated diabetes, the incidence of atherosclerosis is increasing worldwide. Atherosclerosis is a chronic inflammatory disease characterized by the hardening and narrowing of arteries with plaques that consist of inflammatory cells, dead endothelial cells, lipids, and often hyper proliferated vascular smooth muscle cells. During the development of atherosclerosis, vascular endothelial cell (EC) apoptosis induced by the adipokine tumor necrosis factor alpha (TNF‐α), is an early event in the plaque formation. However, TNF‐α alone is not sufficient to induce apoptosis of endothelial cells. Recent studies suggested that the matricellular protein CCN family member 1 (CCN1) involves in endothelial cell dysfunction besides its well‐known angiogenic function during tissue repair by promoting vascular smooth muscle cells proliferation and migration. Herein, we explored the possibility and mechanism of CCN1 in TNF‐α induced endothelial cells apoptosis. Both mRNA and protein levels of CCN1 are found up‐regulated in endothelial cells after TNF‐α treatment. In addition, overexpression of CCN1 promoted endothelial cell apoptosis in the presence of TNF‐α. Furthermore, CCN1 directly up‐regulated the expression of TNF‐α‐target genes, and this up‐regulation required the activation of P53 and NF‐κB both in vivo and in vitro. Taken together, CNN1 regulates TNF‐α induced endothelial cells apoptosis that may underlie poor response to TNF‐α therapy and hence may be a better therapeutic target for preventing vascular dysfunction in obesity.
Different expression levels of spartin cause broad spectrum of cellular consequences in human neuroblastoma cells
Authors: Milewska Malgorzata , Byrne Paula Catherine 
Published: March 2015

AbstractHereditary spastic paraplegia describes a diverse group of neurodegenerative conditions characterised by progressive spasticity and weakness of the lower limbs. Mutations in the SPG20 gene encoding spartin cause an autosomal recessive hereditary spastic paraplegia known as Troyer syndrome. To evaluate the cellular consequences of sustained spartin depletion in neuronal cells, we established several clonal SH‐SY5Y cell lines with different level of spartin knockdown. Here, we report that cells with modest spartin downregulation show signs of neuronal differentiation such as increased neuritogenesis and cytoskeleton rearrangement. Interestingly, we also indicate that permanent high level spartin depletion results in impaired cell growth and multiple mitochondrial aberrations, which we speculate, arise as a result of chronic oxidative stress. Our studies demonstrate that the scale of spartin downregulation is the major factor that determines the severity of cellular consequences observed and suggest that there is a critical level of spartin expression which must be maintained for proper cellular functions.
Contribution of Deep Eutectic Solvents for Biomass Processing: Opportunities, Challenges, and Limitations
Authors: Vigier Karine De Oliveira , Chatel Gregory , Jérôme François 
Published: March 2015

AbstractSince their introduction in 2004, deep eutectic solvents (DESs) have attracted interest in different fields of chemistry. In the case of biomass, DESs are particularly interesting, not only because of their low cost and biodegradability, but also due to their unique property to interact with hydrogen‐bond donors such as renewably sourced polyols or carboxylic acids. Through selected examples, we report the contribution of DESs for the dissolution of biomass, the extraction/purification of bioderived chemicals, and the conversion of carbohydrates to furanic derivatives. In all examples, we discuss how the DES can impact the selectivity of a chemical process and the limitations associated with the use of DES. In particular, impact of the DES viscosity on extraction, the recovery and recycling of DES, and the stabilization of chemical intermediates in DES are among the key aspects that are discussed.
Ruthenium(II) Complexes Containing Lutidine‐Derived Pincer CNC Ligands: Synthesis, Structure, and Catalytic Hydrogenation of CN bonds
Authors: Hernández‐Juárez Martín , López‐Serrano Joaquín , Lara Patricia , Morales‐Cerón Judith P. , Vaquero Mónica , Álvarez Eleuterio , Salazar Verónica , Suárez Andrés 
Published: March 2015

AbstractA series of Ru complexes containing lutidine‐derived pincer CNC ligands have been prepared by transmetalation with the corresponding silver‐carbene derivatives. Characterization of these derivatives shows both mer and fac coordination of the CNC ligands depending on the wingtips of the N‐heterocyclic carbene fragments. In the presence of tBuOK, the Ru‐CNC complexes are active in the hydrogenation of a series of imines. In addition, these complexes catalyze the reversible hydrogenation of phenantridine. Detailed NMR spectroscopic studies have shown the capability of the CNC ligand to be deprotonated and get involved in ligand‐assisted activation of dihydrogen. More interestingly, upon deprotonation, the Ru‐CNC complex 5 e(BF4) is able to add aldimines to the metal–ligand framework to yield an amido complex. Finally, investigation of the mechanism of the hydrogenation of imines has been carried out by means of DFT calculations. The calculated mechanism involves outer‐sphere stepwise hydrogen transfer to the CN bond assisted either by the pincer ligand or a second coordinated H2 molecule.
Mechanofluorochromic Materials Based on Aggregation‐Induced Emission‐Active Boron Ketoiminates: Regulation of the Direction of the Emission Color Changes
Authors: Yoshii Ryousuke , Suenaga Kazumasa , Tanaka Kazuo , Chujo Yoshiki 
Published: March 2015

AbstractWe report a series of mechanofluorochromic (MFC) compounds based on organoboron complexes with aggregation‐induced emission (AIE) characteristics. We synthesized a variety of boron ketoiminates and investigated the effect of the substituents on the optical properties by altering the end groups in the compounds. The synthesized boron ketoiminates showed AIE properties and MFC behavior. Interestingly, the hypsochromic and bathochromic shifts of the emission bands individually observed for boron ketoiminates depended on the chemical structures of the end groups. From the X‐ray diffraction and differential scanning calorimetry analyses, it was confirmed that the MFC property of boron ketoiminates should be derived from a phase transition between crystalline and amorphous states. In addition, the direction of the peak shifts of the emission bands was controlled by the degree of steric hindrance of the end group.
Enantioselective Allylation of (2E,4E)‐2,4‐Dimethylhexadienal: Synthesis of (5R,6S)‐(+)‐Pteroenone
Authors: Koukal Petr , Kotora Martin 
Published: March 2015

AbstractAllylation, trans‐ and cis‐crotylation of (2E,4E)‐2,4‐dimethylhexadienal, a representative α,β,γ,δ‐unsaturated aldehyde, was carried out under different catalytic and stoichiometric conditions. The reactions catalyzed by organocatalysts TRIP‐PA and N,N′‐dioxides gave the best results with respect to yields, asymmetric induction, and catalyst load in comparison to other procedures. The developed methodology was applied in the enantioselective synthesis of (5R,6S)‐(+)‐pteroenone, a defensive metabolite (ichthyodeterrent) of the Antarctic pteropod Clione antarctica.
Organomagnesium‐Catalyzed Isomerization of Terminal Alkynes to Allenes and Internal Alkynes
Authors: Rochat Raphaël , Yamamoto Koji , Lopez Michael J. , Nagae Haruki , Tsurugi Hayato , Mashima Kazushi 
Published: March 2015

AbstractOrganomagnesium complexes 2 were synthesized from N,N‐dialkylamineimine ligands 1 and dibenzylmagnesium by benzylation of the imine moiety. 3‐Aryl‐1‐propynes reacted with 2 to form the corresponding tetraalkynyl complexes, which acted as catalysts for the transformation of these terminal alkynes into allenes and further to internal alkynes under mild conditions. To the best of our knowledge, this example is the first of an organomagnesium‐catalyzed isomerization of alkynes. Notably, the reactions proceeded through temporally separated autotandem catalysis, thus allowing the isolation of the allene or internal alkyne species in good yields. Mechanistic experiments suggested that the catalytically active tetraalkynyl complexes consist of a tautomeric mixture of alkynyl‐, allenyl‐, and propargylmagnesium species.
Structural, Spectroscopic and Computational Examination of the Dative Interaction in Constrained Phosphine–Stibines and Phosphine–Stiboranes
Authors: Chalmers Brian A. , Bühl Michael , Athukorala Arachchige Kasun S. , Slawin Alexandra M. Z. , Kilian Petr 
Published: March 2015

AbstractA series of phosphine–stibine and phosphine–stiborane peri‐substituted acenaphthenes containing all permutations of pentavalent groups SbClnPh4–n (5–9), as well as trivalent groups SbCl2, Sb(R)Cl, and SbPh2 (2–4, R=Ph, Mes), were synthesised and fully characterised by single crystal diffraction and multinuclear NMR spectroscopy. In addition, the bonding in these species was studied by DFT computational methods. The P–Sb dative interactions in both series range from strongly bonding to non‐bonding as the Lewis acidity of the Sb acceptor is decreased. In the pentavalent antimony series, a significant change in the P–Sb distance is observed between SbClPh3 and SbCl2Ph2 derivatives 6 and 7, respectively, consistent with a change from a bonding to a non‐bonding interaction in response to relatively small modification in Lewis acidity of the acceptor. In the SbIII series, two geometric forms are observed. The P–Sb bond length in the SbCl2 derivative 2 is as expected for a normal (rather than a dative) bond. Rather unexpectedly, the phosphine–stiborane complexes 5–9 represent the first examples of the σ4P→σ6Sb structural motif.
Orienting Tetramolecular G‐Quadruplex Formation: The Quest for the Elusive RNA Antiparallel Quadruplex
Authors: Mendoza Oscar , Porrini Massimiliano , Salgado Gilmar F. , Gabelica Valérie , Mergny Jean‐Louis 
Published: March 2015

AbstractDNA and RNA G‐quadruplexes (G4) are unusual nucleic acid structures involved in a number of key biological processes. RNA G‐quadruplexes are less studied although recent evidence demonstrates that they are biologically relevant. Compared to DNA quadruplexes, RNA G4 are generally more stable and less polymorphic. Duplexes and quadruplexes may be combined to obtain pure tetrameric species. Here, we investigated whether classical antiparallel duplexes can drive the formation of antiparallel tetramolecular quadruplexes. This concept was first successfully applied to DNA G4. In contrast, RNA G4 were found to be much more unwilling to adopt the forced antiparallel orientation, highlighting that the reason RNA adopts a different structure must not be sought in the loops but in the G‐stem structure itself. RNA antiparallel G4 formation is likely to be restricted to a very small set of peculiar sequences, in which other structural features overcome the formidable intrinsic barrier preventing its formation.
Palladium‐Catalyzed Oxidative Synthesis of Highly Functionalized Ortholactones
Authors: Baddeley Kate L. , Cao Qun , Muldoon Mark J. , Cook Matthew J. 
Published: March 2015

AbstractA palladium‐catalyzed oxidative reaction is reported which converts dihydropyrans to their corresponding ortholactone. The products are formed in good to excellent yields with a very high level of chemoselectivity and functional group tolerance. Mechanistic studies confirm that the reaction proceeds by a Wacker‐type mechanism.
Patterned Paper Sensors Printed with Long‐Chain DNA Aptamers
Authors: Carrasquilla Carmen , Little Jessamyn R. L. , Li Yingfu , Brennan John D. 
Published: March 2015

AbstractThere is growing interest in developing printable paper sensors to enable rapid testing of analytes for environmental, food safety, and clinical applications. A major challenge is to find suitable bioinks that are amenable to high‐speed printing and remain functional after printing. We report on a simple and effective approach wherein an aqueous ink composed of megadalton‐sized tandem repeating structure‐switching DNA aptamers (concatemeric aptamers) is used to rapidly create patterned paper sensors on filter paper by inkjet printing. These concatemeric aptamer reporters remain immobilized at the point of printing through strong adsorption but retain sufficient segmental mobility to undergo structure switching and fluorescence signaling to provide both qualitative and quantitative detection of small molecules and protein targets. The convenience of inkjet printing allows for the patterning of internally referenced sensors with multiplexed detection, and provides a generic platform for on‐demand printing of sensors even in remote locations.
Selective Nitrogen Functionalization of Graphene by Bucherer‐Type Reaction
Authors: Chua Chun Kiang , Sofer Zdeněk , Luxa Jan , Pumera Martin 
Published: March 2015

AbstractThe front cover artwork is provided by Pumera’s group at Nanyang Technological University (Singapore) and Sofer’s group at UCT Prague (Czech Republic). The image shows the amino‐functionalization of graphene by the conversion of hydroxyl to amino functional groups against a backdrop of aerial view of New Zealand coastline. Read the full text of the article at 10.1002/chem.201405748.
ChemInform Abstract: Structural Characterization of a New Vacancy Ordered Perovskite Modification Found for Ba3Fe3O7F (BaFeO2.333F0.333): Towards Understanding of Vacancy Ordering for Different Perovskite‐Type Ferrites.
Authors: Clemens Oliver 
Published: March 2015

AbstractThe vacancy ordered perovskite‐type Ba3Fe3O7F (BaFeO2.33F0.33) is prepared by topochemical low‐temperature fluorination of Ba2Fe2O5 using stoichiometric amounts of polyvinylidene difluoride (370 °C, 12 h) followed by annealing in Ar for a defined O content (450 °C, 24 h).
ChemInform Abstract: Crystal Structure and Electronic Properties of Y3AlC3.
Authors: Kotyrba Martin R. , Cuervo‐Reyes Eduardo , Nesper Reinhard 
Published: March 2015

AbstractSingle crystals of Y3AlC3 are prepared from the elements in a Li metal flux in the ratio Li:Y:Al:C = 36:2:2:3 (Nb ampoule, 1250 °C, 7 d; cooling to 600 °C with a rate of 2 °C/h).
ChemInform Abstract: Synthesis, Crystal Structure, and TEM Analysis of Sr19Li44 and Sr3Li2: A Reinvestigation of the Sr—Li Phase Diagram.
Authors: Smetana Volodymyr , Kienle Lorenz , Duppel Viola , Simon Arndt 
Published: March 2015

AbstractSingle crystals of Sr19Li44 (I) and Sr3Li2 (II) are prepared from the elements in Ar (Ta ampoules, 1.
ChemInform Abstract: Syntheses of Two Vanadium Oxide—Fluoride Materials that Differ in Phase Matchability.
Authors: Donakowski Martin D. , Gautier Romain , Lu Hongcheng , Tran T. Thao , Cantwell Jacqueline R. , Halasyamani P. Shiv , Poeppelmeier Kenneth R. 
Published: March 2015

AbstractNon‐centrosymmetric NaVOF4(H2O) (I) and NaVO2‐xF2+x (II; x = 1/3) are prepared from an 1:1:4 molar mixture of V2O5, NaCl, and HF (teflon container, 100 °C, 30 min for (I) and 150 °C, 24 h for (II)).
ChemInform Abstract: A Facile Strategy to Achieve High Conduction and Excellent Chemical Stability of Lithium Solid Electrolytes.
Authors: Song Shufeng , Lu Jia , Zheng Feng , Duong Hai M. , Lu Li 
Published: March 2015

AbstractSubstitution of Cl for O based on the concept of bottleneck size and binding energy is performed for Li10.5‐xSi/Ge1.5P1.5Clx O12‐x (x = 0.05‐1).
ChemInform Abstract: Unification of Catalytic Water Oxidation and Oxygen Reduction Reactions: Amorphous Beat Crystalline Cobalt Iron Oxides.
Authors: Indra Arindam , Menezes Prashanth W. , Sahraie Nastaran Ranjbar , Bergmann Arno , Das Chittaranjan , Tallarida Massimo , Schmeisser Dieter , Strasser Peter , Driess Matthias 
Published: March 2015

AbstractControlled solvothermal preparation of highly crystalline CoFe2O4 (I) and amorphous CoFe2On (II; n ≈3.66) are performed by using aqueous resp.
ChemInform Abstract: Synthesis and Characterization of Fluorodinitroamine, FN(NO2)2.
Authors: Christe Karl O. , Wilson William W. , Belanger‐Chabot Guillaume , Haiges Ralf , Boatz Jerry A. , Rahm Martin , Prakash G. K. Surya , Saal Thomas , Hopfinger Mathias 
Published: March 2015

AbstractThe title compound is synthesized by reaction of NF4SbF6 and KN(NO2)2 using SO2 or MeCN as solvent (vacuum, ‐196 °C followed by warming to the melting point of the solvent) and characterized by multinuclear NMR and Raman spectroscopy, and quantum chemical DFT calculations.
ChemInform Abstract: Mercury(II) Nitroprusside: A Framework with an Unusual Topology.
Authors: Cano A. , Osiry H. , Reguera L. , Lemus‐Santana A. A. , Reguera E. 
Published: March 2015

AbstractHg[Fe(CN)5NO] is precipitated from aqueous solutions of Na2[Fe(CN)5NO] and Hg(NO3)2 followed by aging in the dark for 3 days.
ChemInform Abstract: High Pressure Synthesis and Properties of Bi0.5Pb0.5CrO3: A Novel Cr4+/Cr3+ Perovskite.
Authors: Pirrotta Ivan , Schmidt Rainer , Dos Santos‐Garcia Antonio J. , Garcia‐Hernandez Mar , Moran Emilio , Alario‐Franco Miguel. A. 
Published: March 2015

AbstractThe Bi0.5Pb0.5CrO3 double perovskite is prepared by high pressure reaction of stoichiometric amounts of PbO, Bi2O3, Cr2O3, and CrO2 (Pt capsule in a belt‐type press, 70 kbar, 1000 °C, 15 min).
ChemInform Abstract: A Strategy for the Preparation of Thioantimonates Based on the Concept of Weak Acids and Corresponding Strong Bases.
Authors: Anderer Carolin , Delwa de Alarcon Natalie , Naether Christian , Bensch Wolfgang 
Published: March 2015

AbstractThe layered compounds (III) and (V) are synthesized by a new hydrothermal synthetic approach which is based on the in situ formation of a basic medium by the reaction between the strong base SbS43‐ and the weak acid H2O.
ChemInform Abstract: Highly Diastereoselective Generation of Various 3,3‐Disubstituted Allyl Boronates for the Stereospecific Construction of Quaternary Centers.
Authors: Biggs Robyn Aryn , Lambadaris Maria , Ogilvie William W. 
Published: March 2015

AbstractVarious dialkyl substituted allyl boronates are condensed with aromatic and aliphatic aldehydes.
ChemInform Abstract: Organocatalytic Asymmetric Vinylogous Michael Addition of 3‐Alkylidene Oxindoles to α‐Substituted β‐Nitroacrylates: Facile Construction of a Chiral All‐Carbon Quaternary Center.
Authors: Zhong Yuan , Ma Shixiong , Xu Zhaoqing , Chang Min , Wang Rui 
Published: March 2015

AbstractThe stereocenters and alkene geometries of the products (III), (V), and (VII) are precisely controlled by the employed bifunctional squaramide catalyst.
ChemInform Abstract: Gallium‐Assisted Transfer Hydrogenation of Alkenes.
Authors: Michelet Bastien , Bour Christophe , Gandon Vincent 
Published: March 2015

AbstractOptimized reaction conditions including 1,4‐cyclohexadiene as hydrogen source in combination with a gallium carbene complex catalyst allow hydrogenative cyclization, hydroarylation, and reduction of olefins.
ChemInform Abstract: Catalytic and Direct Methyl Sulfonylation of Alkenes and Alkynes Using a Methyl Sulfonyl Radical Generated from a DMSO, Dioxygen and Copper System.
Authors: Jiang Yaojia , Loh Teck‐Peng 
Published: March 2015

AbstractAlkyl substituted alkene analogues of (I) are unreactive.
ChemInform Abstract: Sulfonamide‐Promoted Palladium(II)‐Catalyzed Alkylation of Unactivated Methylene C(sp3)—H Bonds with Alkyl Iodides.
Authors: Chen Kai , Shi Bing‐Feng 
Published: March 2015

AbstractThe Pd(II)‐catalyzed alkylation of unactivated β‐methylene C(sp3)‐H bonds of α‐amino acid substrates having a 8‐aminoquinoline amide auxiliary is described.
ChemInform Abstract: Gold‐Catalyzed Hydroarylation of Alkenes with Dialkylanilines.
Authors: Hu Xingbang , Martin David , Melaimi Mohand , Bertrand Guy 
Published: March 2015

AbstractAnti‐Bredt di(amino)carbene supported gold(I) chloride complexes in combination with KB(C6F5)4 as chloride scavenger promote the hydroarylation of styrenes, norbornene, and enones with dialkylanilines in fair to high yields with good para‐selectivity.
ChemInform Abstract: Chelating Ruthenium Phenolate Complexes: Synthesis, General Catalytic Activity, and Applications in Olefin Metathesis Polymerization.
Authors: Kozlowska Anna , Dranka Maciej , Zachara Janusz , Pump Eva , Slugovc Christian , Skowerski Krzysztof , Grela Karol 
Published: March 2015

AbstractRu‐Complexes (I) and (II) are prepared and investigated in ring‐closing, enyne, and cross‐metathesis reactions.
ChemInform Abstract: Homologation of Isocyanates with Lithium Carbenoids: A Straightforward Access to α‐Halomethyl‐ and α,α‐Dihalomethylamides.
Authors: Pace Vittorio , Castoldi Laura , Mamuye Ashenafi , Holzer Wolfgang 
Published: March 2015

AbstractIn the case of isocyanates with optically active residues, full preservation of chiral information is observed.
ChemInform Abstract: New Approach to α‐Pentafluorosulfanyl‐Substituted Carboxylic Acid Derivatives via Ireland‐Claisen Rearrangements.
Authors: Dreier Anna‐Lena , Matsnev Andrej V. , Thrasher Joseph S. , Haufe Guenter 
Published: March 2015

AbstractAllylic esters (III) undergo Ireland—Claisen rearrangements to form γ,δ‐unsaturated α‐pentafluorosulfanyl carboxylic acids, which are isolated as their methyl esters (V).
ChemInform Abstract: N‐Heterocyclic Carbene‐Catalyzed Enantioselective Synthesis of Functionalized Cyclopentenes via α,β‐Unsaturated Acyl Azoliums.
Authors: Mondal Santigopal , Yetra Santhivardhana Reddy , Patra Atanu , Kunte Sunita S. , Gonnade Rajesh G. , Biju Akkattu T. 
Published: March 2015

AbstractHighly enantioselective NHC‐organocatalyzed synthesis of functionalized cyclopentenes proceeding via α,β‐unsaturated acyl azolium intermediates is reported.