bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enantioanalysis of Pipecolic Acid with Stochastic and Potentiometric Microsensors
Authors: Stefan‐van Staden Raluca‐Ioana , Moldoveanu Iuliana , Sava Daniel‐Florin , Kapnissi‐Christodoulou Constantina , van Staden Jacobus Frederick 
Published: November 2012

ABSTRACTStochastic and potentiometric microsensors based on porphyrins and polymeric surfactants such as polysodium N‐undecanoyl‐ l‐leucylvanilate and polysodium N‐undecanoyl‐ l‐vanilate were developed for enantioselective assay of pipecolic acid. The matrices used for the design of the stochastic sensors were diamond paste and graphite paste, while the matrix used for the design of potentiometric sensors was carbon paste. The response characteristics of the microsensors were determined for the enantiomers of pipecolic acid. The response characteristics, selectivity, and enantioselectivity studies proved that the proposed microsensors can be used for clinical enantioanalysis of pipecolic acid in biological fluids, e.g., urine and whole blood. Chirality 25:114–118, 2013 © 2012 Wiley Periodicals, Inc.
Regional variation in mercury and stable isotopes of red snapper (Lutjanus campechanus) in the northern gulf of Mexico, USA
Authors: Zapp Sluis Michelle , Boswell Kevin M. , Chumchal Matthew M. , Wells R.J. David , Soulen Brianne , Cowan James H. 
Published: November 2012

Abstract
The presence of total mercury (Hg) in fish tissue and the potential associated health risks has become a global concern in marine ecosystems. Few studies have examined basin-scale variation in Hg accumulation in marine ecosystems, and determining if Hg concentrations in fish tissue vary across marine ecosystems is a key monitoring question. The present study evaluated Hg concentrations in red snapper (Lutjanus campechanus) tissue across three regions of the northern Gulf of Mexico (Alabama, Louisiana, and Texas, USA) and between two habitat types (oil and gas platforms and nonplatforms) within each region. Nitrogen (δ15N), carbon (δ13C), and sulfur (δ34S) stable isotopes were used to investigate ecological differences that may affect Hg concentrations among regions and between habitats. Mercury concentrations in red snapper tissue were positively correlated with fish total length. Regional differences in Hg concentrations were significant, with fish collected from Alabama having the highest concentrations and fish collected from Louisiana having the lowest. No significant difference existed in Hg concentrations between habitats, suggesting that association with platforms may not be a significant factor contributing to red snapper Hg concentrations. While δ15N did not differ significantly among the three regions, Texas red snapper were more enriched in δ34S and depleted in δ13C compared with Alabama and Louisiana red snapper. Although the majority of red snapper collected in the present study had Hg concentrations below safe consumption guidelines, regional differences suggest that spatially explicit monitoring programs may be important for basin-wide assessments. Environ. Toxicol. Chem. 2013;32:434–441. © 2012 SETAC
Development of an enzyme-linked immunosorbent assay and a beta-1 adrenergic receptor–based assay for monitoring the drug atenolol
Authors: Sapir A. , Shalev A. Hariton , Skalka N. , Bronshtein A. , Altstein M. 
Published: November 2012

Abstract
Two approaches for monitoring atenolol (ATL) were applied: an immunochemical assay and a competitive-binding assay, based on the interaction between ATL and its target receptor, β1 adrenergic receptor (β1AR). Polyclonal antibodies (Abs) for ATL were generated, and a highly specific microplate immunochemical assay, that is, an enzyme-linked immunosorbent assay (ELISA), for its detection was developed. The ATL ELISA exhibited I50 and limit of detection (I20) values of 0.15 ± 0.048 and 0.032 ± 0.016 ng/ml, respectively, and the Abs did not cross-react with any of the tested beta-blocker drugs. Furthermore, a human β1AR (h-β1AR) was stably expressed in Spodoptera frugiperda cells (Sf9). The receptor was employed to develop a competitive-binding assay that monitored binding of ATL in the presence of isoproteranol by quantification of secondary messenger, cyclic adenosine monophosphate (cAMP), levels in the transfected cells. The assay showed that the recombinant h-β1AR was functional, could bind the agonistic ligand isoproterenol as well as the antagonist ATL, as indicated by a dose-dependent elevation of cAMP in the presence of isoproteranol, and decrease after ATL addition. The highly efficient and sensitive ELISA and the receptor assay represent two methods suitable for efficient and cost-effective large-scale, high-throughput monitoring of ATL in environmental, agricultural, and biological samples. Environ. Toxicol. Chem. 2013;32:585–593. © 2012 SETAC
Mitigating with macrophytes: Submersed plants reduce the toxicity of pesticide-contaminated water to zooplankton
Authors: Brogan William R. , Relyea Rick A. 
Published: November 2012

Abstract
In ecotoxicology, appreciation is growing for the influence that ecological interactions have on the toxicity of contaminants, such as insecticides, to sensitive species. Most previous studies, however, have focused on factors that exacerbate insecticide effects on species, while factors that may mitigate these effects have been relatively ignored. In aquatic habitats, a small number of studies have shown that submersed macrophytes can remove some insecticides from the water column via sorption. Although examining sorption dynamics is important for understanding the environmental fate of insecticides, whether and to what extent macrophytes actually mitigate insecticide effects on aquatic species remains unknown. In the present study, the authors examined how much and how quickly several realistic densities of the macrophyte Elodea canadensis decreased the toxicity of the insecticide malathion to Daphnia magna, a keystone aquatic herbivore. To do this, the authors quantified Daphnia survival in outdoor test systems (0.95 L) exposed to a factorial combination of five Elodea densities crossed with five malathion concentrations. The authors discovered that malathion's lethality to Daphnia decreased with increasing Elodea density. Furthermore, the rate at which Elodea reduced malathion's toxicity in the water column increased with macrophyte density. These results provide strong evidence that submersed macrophytes can mitigate the ecological impacts of a popular insecticide and further support that ecological interactions can strongly influence contaminant environmental effects. Environ. Toxicol. Chem. 2013;32:699–706. © 2012 SETAC
Assessing the sublethal effects of in-river concentrations of parameters contributing to cumulative effects in the athabasca river basin using a fathead minnow bioassay
Authors: Squires Allison J. , Dubé Monique G. , Rozon-Ramilo Lisa D. 
Published: November 2012

Abstract
The Athabasca River basin, located in Alberta, Canada, covers 157, 000 km2 and holds significant cultural and economic importance. Recent research assessed changes in several water quality and quantity parameters that have changed both spatially (along the river continuum) and temporally (pre-development and present day) in the Athabasca River Basin. In particular, parameters such as salinity and dissolved sulphate have changed significantly across the Athabasca River mainstem over the past five decades. Further laboratory testing has linked concentrations of these parameters to changes in fathead minnow reproduction. Research is required to determine whether these changes observed in the laboratory can be applied to actual in-river conditions. The objectives of the present study were to twofold: assess changes in fathead minnow response metrics (i.e., condition, liver and gonad size, egg production, and gill histology) associated with increasing concentrations of salinity and dissolved sulphate and determine whether sublethal effect thresholds established in laboratory experiments correspond to actual in-river concentrations using water from the mouth and headwaters of the Athabasca River. Three dose−response experiments (NaCl, SO4, and water sampled from the mouth of the Athabasca River) were conducted at Jasper National Park, Alberta, Canada. Significant increases in mean eggs per female per day occurred at the 50% treatment for the mouth experiment and thresholds previously developed in the laboratory were verified. Environ. Toxicol. Chem. 2013;32:662–672. © 2012 SETAC
Avoidance of contaminated sediments by an amphipod (Melita plumulosa), A harpacticoid copepod (Nitocra spinipes), and a snail (Phallomedusa solida)
Authors: Ward Daniel J. , Simpson Stuart L. , Jolley Dianne F. 
Published: November 2012

Abstract
The distribution of contaminants is seldom homogeneous in aquatic systems. In the present study, the avoidance response of Melita plumulosa, Nitocra spinipes, and Phallomedusa solida when exposed to contaminated sediments was investigated. Test vessels were designed to allow the congruent placement of two sediments and assessment of the movement of organisms between the sediments. When exposed to reference sediment, each species dispersed evenly between test chambers regardless of differences in sediment particle size. In the presence of contaminated sediment, the magnitude and rate of avoidance varied. Avoidance assays showed that test species avoided contaminated sediment as early as 6, 6, and 24 h following exposure for N. spinipes, P. solida, and M. plumulosa, respectively. The 48-h avoidance response of M. plumulosa for nine contaminated sediments of varying toxicity showed that avoidance was generally greater for sediments which elicited greater 10-d lethality to this species. The study demonstrated that each of these species has the ability to respond to chemical cues in the environment to inhabit sediment that provides the best opportunity for survival. The avoidance response for each species indicates the potential for developing rapid screening methods to assess sediment quality. Evidence suggests that avoidance was related to sediment toxicity and that static 10-d toxicity methods are likely to overestimate toxicity for species, which would avoid contamination in heterogeneous field settings. Environ. Toxicol. Chem. 2013;32:644–652. © 2012 SETAC
Spatial patterns of groundwater‐lake exchange – implications for acid neutralization processes in an acid mine lake
Authors: Neumann Christiane , Beer Julia , Blodau Christian , Peiffer Stefan , Fleckenstein Jan H. 
Published: November 2012

AbstractExchange of groundwater and lake water with typically quite different chemical composition is an important driver for biogeochemical processes at the groundwater‐lake interface, which can affect the water quality of lakes. This is of particular relevance in mine lakes where anoxic and slightly acidic groundwater mixes with oxic and acidic lake water (pH < 3). To identify links between groundwater‐lake exchange rates and acid neutralization processes in the sediments, exchange rates were quantified and related to pore‐water pH, sulfate and iron concentrations as well as sulfate reduction rates within the sediment. Seepage rates measured with seepage meters (−2.5 to 5.8 L m‐2 d‐1) were in reasonable agreement with rates inverted from modeled chloride profiles (−1.8 to 8.1 L m‐2 d‐1). Large‐scale exchange patterns were defined by the (hydro)geologic setting but superimposed by smaller scale variations caused by variability in sediment texture. Sites characterized by groundwater upwelling (flow into the lake) and sites where flow alternated between upwelling and downwelling were identified. Observed chloride profiles at the alternating sites reflected the transient flow regime. Seepage direction, as well as seepage rate, were found to influence pH, sulfate and iron profiles and the associated sulfate reduction rates. Under alternating conditions proton‐consuming processes, for example, sulfate reduction, were slowed. In the uppermost layer of the sediment (max. 5 cm), sulfate reduction rates were significantly higher at upwelling (>330 nmol g‐1 d‐1) compared to alternating sites (<220 nmol g‐1 d‐1). Although differences in sulfate reduction rates could not be explained solely by different flux rates, they were clearly related to the prevailing groundwater‐lake exchange patterns and the associated pH conditions. Our findings strongly suggest that groundwater‐lake exchange has significant effects on the biogeochemical processes that are coupled to sulfate reduction such as acidity retention and precipitation of iron sulfides. Copyright © 2012 John Wiley & Sons, Ltd.
Morphometric, quantitative, and three‐dimensional analysis of the heart muscle fibers of old rats: Transmission electron microscopy and high‐resolution scanning electron microscopy methods
Authors: Cury Diego Pulzatto , Dias Fernando José , Sosthenes Marcia Consentino Kronka , Dos Santos Haemmerle Carlos Alexandre , Ogawa Koichi , Da Silva Marcelo Cavenaghi Pereira , Mardegan Issa João Paulo , Iyomasa Mamie Mizusaki , Watanabe Ii‐Sei 
Published: November 2012

AbstractThis research investigated the morphological, morphometric, and ultrastructural cardiomyocyte characteristics of male Wistar rats at 18 months of age. The animals were euthanized using an overdose of anesthesia (ketamine and xylazine, 150/10 mg/kg) and perfused transcardially, after which samples were collected for light microscopy, transmission electron microscopy, and high‐resolution scanning electron microscopy. The results showed that cardiomyocyte arrangement was disposed parallel between the mitochondria and the A‐, I‐, and H‐bands and their M‐ and Z‐lines from the sarcomere. The sarcomere junction areas had intercalated disks, a specific structure of heart muscle. The ultrastructural analysis revealed several mitochondria of various sizes and shapes intermingled between the blood capillaries and their endothelial cells; some red cells inside vessels are noted. The muscle cell sarcolemma could be observed associated with the described structures. The cardiomyocytes of old rats presented an average sarcomere length of 2.071 ± 0.09 μm, a mitochondrial volume density (Vv) of 0.3383, a mitochondrial average area of 0.537 ± 0.278 μm2, a mitochondrial average length of 1.024 ± 0.352 μm, an average mitochondrial cristae thickness of 0.038 ± 0.09 μm and a ratio of mitochondrial greater length/lesser length of 1.929 ± 0.965. Of the observed mitochondrial shapes, 23.4% were rounded, 45.3% were elongated, and 31.1% had irregular profiles. In this study, we analyzed the morphology and morphometry of cardiomyocytes in old rats, focusing on mitochondria. These data are important for researchers who focus the changes in cardiac tissue, especially changes owing to pathologies and drug administration that may or may not be correlated with aging. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.
Complex partial nasal reconstruction using free prelaminated temporoparietal fascial flap
Authors: Cinpolat Ani , Bektas Gamze , Coskunfirat O. Koray 
Published: November 2012

AbstractThe reconstruction of nasal defects together with nasal lining, skeletal support, and skin loss constitutes difficulty to plastic surgeons. We present a single‐stage reconstruction of the defect formed on the nasal tip, columella, septum, and upper lip after tumor excision by performing free temporoparietal fascial flap, costal cartilage, and skin graft. In this case, cartilage support was created by the graft taken from costal cartilage, and free temporoparietal fascial flap was wrapped around this cartilage scaffold. Skin graft taken from scalp was placed on the skin surface, and skin graft taken from the thigh was placed on the mucosal surface. Vascular anastomoses were performed on the labial artery and the concomitant vein. In consequence of this operation, a nasal reconstruction with acceptable esthetic and functional results was provided in a complex nasal defect. Internal lining, skin, and cartilage structures were replaced in one single stage and with single flap and graft. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.
Brief report: Improving the validity of assessments of adolescents' feelings of privacy invasion
Authors: Laird Robert D. , Marrero Matthew D. , Melching Jessica , Kuhn Emily S. 
Published: November 2012

ABSTRACTStudies of privacy invasion have relied on measures that combine items assessing adolescents' feelings of privacy invasion with items assessing parents' monitoring behaviors. Removing items assessing parents' monitoring behaviors may improve the validity of assessments of privacy invasion. Data were collected from 163 adolescents (M age 13 years, 5 months; 47% female; 50% European American, non‐Hispanic, 46% African American) and their mothers. A model specifying separate factors for privacy invasion and monitoring behavior fit adolescent‐reported and parent‐reported data significantly better than a single factor model. Although privacy invasion and monitoring behavior were positively associated, privacy invasion and monitoring behavior correlations were significantly different from one another across all ten variables reported by adolescents and across eight of the nine variables reported by mothers. The pattern of results strongly supports a recommendation for researchers to exclude items assessing monitoring behaviors to provide a more valid assessment of privacy invasion.
Overgeneral autobiographical memory, emotional maltreatment, and depressive symptoms in adolescence: Evidence of a cognitive vulnerability–stress interaction☆
Authors: Stange Jonathan P. , Hamlat Elissa J. , Hamilton Jessica L. , Abramson Lyn Y. , Alloy Lauren B. 
Published: November 2012

ABSTRACTOvergeneral autobiographical memory (OGM) is associated with depression and may confer risk for the development of depressed mood, but few longitudinal studies have evaluated OGM as a predictor of depressive symptoms in early adolescence, particularly in the context of environmental stressors. We investigated whether OGM and emotional maltreatment would interact to predict prospective increases in depressive symptoms in early adolescents and whether these effects differed by race. Among 174 seventh‐graders, OGM and familial emotional abuse interacted to predict depressive symptoms eight months later, controlling for initial depressive symptoms. Specifically, emotional abuse predicted increases in depressive symptoms among Caucasian adolescents with more OGM, but not among those with less OGM. This association was not significant for African American adolescents. These results provide support for a cognitive vulnerability–stress relationship between OGM and emotional abuse in early adolescence and suggest that these mechanisms of risk for depression may be specific to Caucasian adolescents.
Gestational doxorubicin alters fetal thyroid–brain axis
Authors: Ahmed R.G. , Incerpi S. 
Published: November 2012

AbstractAdministration of chemotherapy during pregnancy may represent a big risk factor for the developing brain, therefore we studied whether the transplacental transport of doxorubicin (DOX) may affect the development of neuroendocrine system. DOX (25 mg/kg; 3 times interaperitoneally/week) was given to pregnant rats during whole gestation period. The disturbances in neuroendocrine functions were investigated at gestation day (GD) 15 and 20 by following the maternal and fetal thyroid hormone levels, fetal nucleotides (ATP, ADP, AMP) levels and adenosine triphosphatase (Na+, K+‐ATPase, Ca2+‐ATPase and Mg2+‐ATPase) activities in two brain regions, cerebrum and cerebellum. In control group, the levels of maternal and fetal serum thyroxine (T4), triiodothyronine (T3), thyrotropin (TSH), and fetal serum growth hormone (GH) increased from days 15 to 20, whereas in the DOX group, a decrease in maternal and fetal T4, T3 and increase in TSH levels (hypothyroid status) were observed. Also, the levels of fetal GH decreased continuously from GD 15 to 20 with respect to control group. In cerebrum and cerebellum, the levels of fetal nucleotides and the activities of fetal ATPases in control group followed a synchronized course of development. The fetal hypothyroidism due to maternal administration of DOX decreased the levels of nucleotides, ATPases activities, and total adenylate, instead, the adenylate energy charge showed a trend to an increase in both brain regions at all ages tested. These alterations were dose‐ and age‐dependent and this, in turn, may impair the nerve transmission. Finally, DOX may act as neuroendocrine disruptor causing hypothyroidism and fetal brain energetic dysfunction.
Effect of body mass index on clinical outcomes of patients undergoing total laparoscopic hysterectomy
Authors: Morgan‐Ortiz Fred , Soto‐Pineda Juan M. , López‐Zepeda Marco A. , de Jesús Peraza‐Garay Felipe 
Published: November 2012

AbstractObjectiveTo evaluate the effect of normal body mass index (BMI, calculated as weight in kilograms divided by the square of height in meters) and obesity on clinical results among patients who underwent total laparoscopic hysterectomy (TLH).MethodsIn a prospective study at the Civil Hospital of Culiacan in Sinaloa State, Mexico, data were compared from 209 patients who underwent TLH between July 6, 2009, and December 30, 2011. The following primary variables were analyzed for 77 normal BMI patients, 82 overweight patients, and 50 obese patients: procedure duration, operative bleeding, major and minor trans‐operative complications, length of hospital stay, and postoperative pain.ResultsThe mean duration of surgical procedure (P < 0.001) and operative bleeding (P = 0.002) were lower for patients with normal BMI compared with the other 2 groups. The rate of conversion to laparotomy was similar among the 3 groups. Overall, the frequency of complications was 6.2% (n = 13/209); the frequency of complications by study group was 2.6%, 4.8%, and 14% for the normal BMI, overweight and obesity groups, respectively (P < 0.05). Major complications were more frequent among patients with obesity (P = 0.010).ConclusionThe duration of surgical procedure and operative morbidity were found to increase, mainly owing to major complications, among patients with obesity (BMI ≥ 30).
Peripartum hysterectomy between 2009 and 2010 in Sichuan, China
Authors: Chen Meng , Zhang Li , Wei Qiang , Fu Xiaodong , Gao Qian , Liu Xinghui 
Published: November 2012

AbstractObjectiveTo investigate the incidence and risk factors for peripartum hysterectomy (PH) in Sichuan, China.MethodsA population‐based study was conducted using the obstetric inpatient records of women who gave birth at 1 of 8 hospitals between January 1, 2009, and December 31, 2010. Multivariate logistic regression analysis was used to identify independent risk factors for PH.ResultsDuring the study period, 64 cases of PH occurred among 34 014 deliveries (0.2%); abnormal placentation and uterine atony were the major indications for surgery. Several factors significantly increased the risk of PH among the study population: placenta previa (adjusted odds ratio [AOR] 10.0; 95% confidence interval [CI], 5.7–17.7), uterine atony (AOR 7.4; 95% CI, 4.3–12.8), repeat cesarean delivery (AOR 3.0; 95% CI, 1.2–7.7), low birth weight (AOR 2.6; 95% CI, 1.5–4.6), and multiparity (AOR 2.4; 95% CI, 1.3–4.4). By contrast, multiple gestation, primary cesarean delivery, placental abruption, leiomyoma, pre‐eclampsia, eclampsia, and diabetes mellitus did not significantly increase the risk of PH.ConclusionPlacenta previa, uterine atony, repeat cesarean delivery, low birth weight, and multiparity were risk factors for PH among the study population.