bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lymphatic Vessels in Peritoneal Endometriotic Lesions
Authors: Hey-Cunningham Alison J. , Berbic Marina , Ng Cecilia H.M. , Markham Robert , Fraser Ian S. 
Published: January 2015

Purpose While the exact pathogenesis of endometriosis remains unclear, it has been hypothesized that fragments of viable endometrial tissue shed at menstruation can transit via the lymphatic circulation and establish endometriotic lesions at distant sites. Mounting evidence suggests that related parameters such as immune cell densities are altered in peritoneal endometriotic lesions. However, lymphatic vessels in lesions have not been previously investigated. In this study, the presence and distribution of lymphatic micro-vessels were characterized in peritoneal endometriotic lesions and surrounding tissues. Methods Immunohistochemical staining was performed with an antibody specific for lymphatic endothelium (podoplanin, D2–40 clone) on 48 peritoneal endometriotic lesion specimens and 15 samples of normal surface peritoneum. Lymphatic micro-vessel density (LVD) was quantified in and around endometriotic lesions, and in normal surface peritoneum using an automated cellular imaging system. Results LVD in endometriotic stroma (mean ± SD = 31.1 ± 28.8 vessels per mm2) was significantly increased compared to both adjacent (8.8 ± 7.6, P<.001) and distant sub-peritoneum (17.7 ± 12.0, P=.002). In addition, lesion-adjacent LVD was significantly lower than distant (P<.001). No statistically significant differences in LVD were observed between endometriotic stroma and normal surface peritoneum from women without endometriosis (31.1 ± 26.5) or at different phases of the menstrual cycle. Conclusions This is the first report of LVD in peritoneal endometriotic lesions. LVD is increased in the stroma of peritoneal lesions compared to the surrounding sub-peritoneal tissue. Lymphatic micro-vessels play an important role in the trafficking of immune cells within endometriotic lesions and may be involved in lesion establishment.
Gelijke behandeling en derdelanders: realiteit of toekomstmuziek?
Authors: Oosterom-Staples H. 
Published: January 2015

Gelijke behandeling en derdelanders: realiteit of toekomstmuziek: De Langdurig-ingezetenerichtlijn in zes arresten
Op 17 juli 2014 deed het Hof van Justitie uitspraak in de zaak Tahir. Dit is het zesde arrest van het Hof van Justitie over de in 2003 door de Raad vastgestelde Langdurig-ingezetenerichtlijn die begin 2006 door de lidstaten geïmplementeerd moest zijn. Het arrest Tahir is de aanleiding voor een bijdrage over deze richtlijn die de verblijfspositie en rechten van langdurig ingezeten derdelanders regelt en hen definieert. Naast het arrest Tahir zal in deze bijdrage ook aandacht zijn voor de andere arresten van het Hof van Justitie over de positie van langdurig ingezeten derdelanders. De vraag die centraal staat, is afgeleid van een van de doelstellingen van de Langdurig-ingezetenerichtlijn: draagt deze bij aan de gelijke behandeling van derdelanders?HvJ 17 juli 2014, zaak C-469/13, Shamim Tahir/Ministero dellÍnterno, Questura di Verona, ECLI:EU:C:2014:2094, n.n.g.
Genèse du cycle de « La batèche » de Gaston Miron : de la noirceur aux rives de l’aube
nouvelle
Authors: Villeneuve Karine 
Published: January 2015

Inspirée de la critique génétique, cette étude lève partiellement le voile sur les
origines de « La batèche », l’une des plus importantes suites poétiques de L’homme rapaillé de Gaston Miron. Les transformations
visibles dans la genèse de ce cycle ont été grandement influencées par le premier séjour du
poète en France, moment décisif au cours duquel il prend la pleine mesure de ses racines et
de la condition canadienne-française. Resté inachevé, le cycle de « La batèche » se compose
uniquement de deux poèmes, mais il regroupe un nombre impressionnant de témoins génétiques
en raison de toutes les modifications apportées aux textes. L’analyse de ces transformations
porte sur les différents états publiés des poèmes, de même que sur l’ensemble des témoins
génétiques associés à cette suite inachevée : les notes personnelles de l’auteur, sa
correspondance, ses entretiens, les fragments de poèmes ou les brouillons déposés à BAnQ. Le
mouvement inscrit dans le cycle s’appuie sur une relation entre la pénombre et la lumière.
Ces deux pôles constitutifs de « La batèche », illustrés dans les poèmes par l’aliénation et
par son dépassement grâce à la revendication, se construisent dans une optique d’affirmation
de soi qui débouche sur un projet de libération collective.
Repercussões do cenário contemporâneo no ato de compartilhar refeições em família
Authors: Kasper Machado Isabel , Becker Débora , Martins de Campos Débora , Welter Wendt Guilherme , Saraiva de Macedo Lisboa Carolina 
Published: January 2015

O presente artigo teve por objetivo discutir as transformações que o ato de se alimentar em família sofreu nas últimas décadas. Com este propósito, discorre-se sobre as influências econômicas e tecnológicas que provocaram variações de comportamento e dos valores na sociedade e, por consequência, na organização do grupo familiar, envolvendo, entre outros processos, a alimentação. Buscou-se fazer leituras que primeiramente auxiliassem a compreensão do ato de alimentar-se, considerando a influência de fenômenos de ordem cultural e histórica, como a industrialização, a crescente urbanização e até mesmo as guerras. A literatura tem sinalizado modificações importantes na família, especialmente em hábitos relacionados à alimentação e aos aspectos emocionais devido às mudanças na sociedade contemporânea. Muito embora esteja claro para os pesquisadores que a comensalidade exerça um papel protetivo para a saúde física e mental dos indivíduos, o que se constata é um crescente aumento da alimentação em restaurantes e lanchonetes, ou seja, fora de casa, o que, em geral, é inadequado e nocivo à saúde. Percebe-se que as famílias convivem com um excesso de informações sobre alimentação saudável e não saudável e têm rotinas desregradas em função de elevadas e crescentes demandas sociais e profissionais. Pesquisadores apontam que os encontros familiares reduziram e este fato pode repercutir nas escolhas e práticas alimentares. Programas interdisciplinares de intervenção com famílias que considerem o cenário atual ainda são escassos, mas extremamente necessários. Sugestões para investigações futuras são apresentadas e discutidas.
Análise de estudos brasileiros sobre o pai e o desenvolvimento infantil
Authors: Kinas Bueno Rovana , Luís Vieira Mauro 
Published: January 2015

Os estudos sobre a relação entre pai e filho e sobre a influência do pai no desenvolvimento da criança têm aumentado nas últimas décadas, mas a produção nacional ainda é reduzida. O objetivo deste estudo foi caracterizar a produção acadêmica brasileira sobre o tema. Realizou-se uma busca sistemática em três bases de dados bibliográficas: Index Psi, PEPsic e Scielo Brasil. Utilizaram-se as palavras-chave: “pai”, “father”, “desenvolvimento infantil”, “desenvolvimento da criança” e “child development”. Selecionou-se 29 obras, que foram analisadas por meio de categorias. A temática mais presente nos estudos foi o comportamento paterno, bem como sua relação com o desenvolvimento infantil. Há um maior número de estudos empíricos, todos transversais. Constata-se a prevalência de pesquisas de levantamento de dados, e sua maioria coletados por meio de questionários e entrevistas. Faz-se necessário a ampliação dos estudos sobre o pai e sua importância no desenvolvimento infantil, mediante construção de diferentes arranjos metodológicos.
Mães de vestibulandos: Sentimentos, percepções e expectativas perante o vestibular do filho
Authors: Da Costa Souto Danielle , Saling Kruel Cristina 
Published: January 2015

O estudo objetivou investigar sentimentos, percepções e expectativas das mães diante do processo de seleção para o ingresso do filho no curso de graduação. Participaram do estudo cinco mães que estavam presentes nos dias de prova e cujos filhos estavam realizando o concurso de vestibular em uma universidade pública no interior do Rio Grande do Sul. Utilizou-se delineamento de pesquisa de cunho qualitativo. As mães foram entrevistadas no momento em que seus filhos estavam realizando as provas, por meio de uma entrevista semiestruturada, sendo indagadas sobre como se sentiam e quais eram suas percepções e expectativas naquela ocasião. A Análise de Conteúdo indicou que as mães participam intensamente dos dias de prova dos filhos e os principais sentimentos por elas expostos foram de ansiedade e nervosismo quanto à espera. Destacou-se a intensa preocupação das mães quanto ao seu papel durante os dias de prova. Conclui-se que a troca de sentimentos entre as mães, existente nos dias de prova, é muito importante para a vivência desse momento. Elas buscam reafirmar entre si seu papel maternal e sua identidade de mãe.
Conduta e análise heurística de idosas no jogo “Cara a Cara”
Authors: Chisté Santos Claudimara , Carlos Ortega Antonio 
Published: January 2015

O presente estudo teve como objetivo: (1) caracterizar os tipos de condutas; (2) especificar os níveis de análise heurística de seis mulheres com idade entre 61 e 67 anos, em um contexto de quatro oficinas do jogo “Cara a Cara”. As categorias analisadas representam um recorte teórico da Teoria da Equilibração de Piaget e foram investigadas de acordo com o método clínico crítico por meio de situações-problema e entrevistas. Em relação aos tipos de conduta, duas participantes tiveram muita facilidade nesse jogo e não manifestaram perturbação suficiente,ou seja, não precisaram reagir ao erro. Por isso, seus tipos não foram avaliados. As demais demonstraram condutas do tipo Beta, exceto uma delas que demonstrou conduta do tipo Alfa, pois, ao alegar problemas de memória, passou a não se importar mais com seus resultados.No que se refere ao nível de análise heurística, a participante que inicialmente cometeu alguns erros foi a que mais evoluiu (II/B), permanecendo as demais em II/A, e uma única participantese manteve no nível I por não gostar do jogo utilizado. [#]
Client Importance: A Measurement Bias and Its Impact *
Authors: Wu Xi , Zeng Tiebing , Wang Chunfei 
Published: January 2015

Abstract
The impact of client importance on audit quality has long been attracting academic and regulatory attention. When measuring client importance, however, researchers usually use the economic benefits from all listed clients (rather than all clients) as a proxy for the revenue base of client importance. Taking advantage of a unique set of data from the Chinese Institute of Certified Public Accountants (CICPA), this study examines the potential impact of such measurement bias on empirical findings. Using the association between client importance and accrual-based earnings management as the test scenario, our evidence shows that when evaluating the economic consequences of client importance, the use of a complete client base in measuring client importance leads to a discernibly different interpretation to that produced using an incomplete client base. Moreover, the measurement bias also has a discernible impact on the interpretation of empirical results at the audit firm office level.
Diagnosing Lysosomal Storage Disorders: Mucopolysaccharidosis Type I
Authors: Johnson Britt A. , Dajnoki Angela , Bodamer Olaf A. 
Published: January 2015

AbstractMucopolysaccharidosis type I (MPS I) is a lysosomal storage disorder due to deficiency of alpha iduronidase (IDUA). Progressive storage of dermatan and heparan sulfate throughout the body lead to a multiorgan presentation including short stature, dysostosis multiplex, corneal clouding, hearing loss, coarse facies, hepatosplenomegaly, and intellectual disability. Diagnosis of MPS I is based on IDUA enzyme analysis in leukocytes or dried blood spots (DBS) followed by molecular confirmation of the IDUA gene mutations in individuals with low enzyme activity. The advent of mass spectrometry methods for enzyme analysis in DBS has enabled high‐throughput screening for MPS I in symptomatic individuals and newborn infants. The following unit provides the detailed analytical protocol for measurement of IDUA activity in DBS using tandem mass spectrometry. © 2015 by John Wiley & Sons, Inc.
Intraplate volcanism at the edges of the Colorado Plateau sustained by a combination of triggered edge‐driven convection and shear‐driven upwelling
Authors: Ballmer Maxim D. , Conrad Clinton P. , Smith Eugene I. , Johnsen Racheal 
Published: January 2015

AbstractAlthough volcanism in the southwestern United States has been studied extensively, its origin remains controversial. Various mechanisms such as mantle plumes, upwelling in response to slab sinking, and small‐scale convective processes have been proposed, but have not been evaluated within the context of rapidly shearing asthenosphere that is thought to underlie this region. Using geodynamic models that include this shear, we here explore spatiotemporal patterns of mantle melting and volcanism near the Colorado Plateau. We show that the presence of viscosity heterogeneity within an environment of asthenospheric shearing can give rise to decompression melting along the margins of the Colorado Plateau. Our models indicate that eastward shear flow can advect pockets of anomalously low viscosity toward the edges of thickened lithosphere beneath the plateau, where they can induce decompression melting in two ways. First, the arrival of the pockets critically changes the effective viscosity near the plateau to trigger small‐scale edge‐driven convection. Second, they can excite shear‐driven upwelling (SDU), in which horizontal shear flow becomes redirected upward as it is focused within the low‐viscosity pocket. We find that a combination of “triggered” edge‐driven convection and SDU can explain volcanism along the margins of the Colorado Plateau, its encroachment toward the plateau's southwestern edge, and the association of volcanism with slow seismic anomalies in the asthenosphere. Geographic patterns of intraplate volcanism in regions of vigorous asthenospheric shearing may thus directly mirror viscosity heterogeneity of the sublithospheric mantle.
Influence of ENSO on the QBO: Results from an ensemble of idealized simulations
Authors: Schirber S. 
Published: January 2015

AbstractThe El Niño–Southern Oscillation (ENSO) changes convection in the tropics and therefore the excitation of equatorial waves. Equatorial waves propagate from the troposphere upward where they drive the quasi‐biennial oscillation (QBO) in the stratosphere. In this work, we analyze the effect of ENSO on the QBO utilizing an atmospheric general circulation model in a comprehensive experimental setup. We construct two ensembles of different QBO initial conditions, with the onset of a westerly (QBOW) and easterly (QBOE) jet at 10 hPa. In the course of an 18 months simulation period, the two sets of initial conditions experience each El Niño (EL) and La Niña (LA) boundary conditions. Due to the increased tropospheric temperatures during EL conditions compared to LA conditions, the experiments show an increase in tropospheric wave activity which increases QBO forcing in the stratosphere in EL. The underlying easterly jet in QBOW is weaker during EL compared to LA, while the underlying westerly jet in QBOE is stronger during EL compared to LA. On one hand, the weaker underlying jet in QBOW and the increase in QBO forcing due to waves cause a faster downward propagation for the westerly jet in QBOW during EL. On the other hand, the stronger underlying jet in QBOE opposes the increased QBO forcing due to waves for QBOE during EL. Therefore, the downward propagation speed of the easterly jet in QBOE is similar during EL and LA conditions. Changes in stratospheric tropical upwelling associated with EL and LA do not affect QBO properties in the simulation.
Investigating diurnal and seasonal climatic response to land use and land cover change over monsoon Asia with the Community Earth System Model
Authors: Xu Zhongfeng , Mahmood Rezaul , Yang Zong‐Liang , Fu Congbin , Su Hua 
Published: January 2015

AbstractLand use and land cover change (LULCC) is primarily characterized as forest conversion to cropland for the development of agriculture. Previous climate modeling studies have demonstrated the LULCC impacts on mean climate and its long‐term trends. This study investigates the diurnal and seasonal climatic response to LULCC in monsoon Asia through two numerical experiments with potential and current vegetation cover using the fully coupled Community Earth System Model. Results show that LULCC leads to a reduced diurnal temperature range due to the enhanced (reduced) diurnal cycle of the ground heat flux (sensible heat flux). Daily minimum surface air temperature (Tmin) exhibits a clear seasonality over India as it increases most in the premonsoon season and least during the summer monsoon season. Similarly, a strong anticyclonic anomaly is present at 850 hPa over India in spring and over eastern China in autumn, but weak changes in circulation appear in winter and summer. In addition, the LULCC results in significant changes in the variability of the 2 m air temperature, as characterized by an enhanced variability in India and a reduced variability in northern China to eastern Mongolia in autumn and winter. Possible land‐atmosphere feedback loops involving surface albedo, soil moisture, evapotranspiration, atmospheric circulation, and precipitation are offered as biogeophysical mechanisms that are responsible for the region‐specific LULCC‐induced diurnal and seasonal response.
Short‐term time step convergence in a climate model
Authors: Wan Hui , Rasch Philip J. , Taylor Mark A. , Jablonowski Christiane 
Published: January 2015

AbstractThis paper evaluates the numerical convergence of very short (1 h) simulations carried out with a spectral‐element (SE) configuration of the Community Atmosphere Model version 5 (CAM5). While the horizontal grid spacing is fixed at approximately 110 km, the process‐coupling time step is varied between 1800 and 1 s to reveal the convergence rate with respect to the temporal resolution. Special attention is paid to the behavior of the parameterized subgrid‐scale physics. First, a dynamical core test with reduced dynamics time steps is presented. The results demonstrate that the experimental setup is able to correctly assess the convergence rate of the discrete solutions to the adiabatic equations of atmospheric motion. Second, results from full‐physics CAM5 simulations with reduced physics and dynamics time steps are discussed. It is shown that the convergence rate is 0.4—considerably slower than the expected rate of 1.0. Sensitivity experiments indicate that, among the various subgrid‐scale physical parameterizations, the stratiform cloud schemes are associated with the largest time‐stepping errors, and are the primary cause of slow time step convergence. While the details of our findings are model specific, the general test procedure is applicable to any atmospheric general circulation model. The need for more accurate numerical treatments of physical parameterizations, especially the representation of stratiform clouds, is likely common in many models. The suggested test technique can help quantify the time‐stepping errors and identify the related model sensitivities.
Directed Evolution of DNA Polymerases: Construction and Screening of DNA Polymerase Mutant Libraries
Authors: Gloeckner Christian , Kranaster Ramon , Marx Andreas 
Published: January 2015

AbstractThe protocols in this article describe the construction of a mutant DNA polymerase library using error‐prone PCR (epPCR) as a method for gene randomization, followed by screening of the library using two different approaches. The examples described use an N‐terminally truncated form of the thermostable DNA polymerase I of Thermus aquaticus (Taq DNA polymerase), namely Klentaq (KTQ), and protocols are included for the identification of variants with (1) increased DNA lesion‐bypass ability and (2) enhanced selectivity for DNA match/mismatch recognition. The screening assays are based on double‐stranded DNA detection (using SYBR Green I) which can be carried out using standard laboratory equipment. The described assays are designed for use in a 384‐well plate format to increase screening throughput and reduce material costs. For improved accuracy and ease of liquid handling, the use of an automated liquid handling device is recommended. Curr. Protoc. Chem Biol. 2:89‐109. © 2010 by John Wiley & Sons, Inc.
Hematology Testing in Mice
Authors: Lundberg Pontus , Skoda Radek 
Published: January 2015

AbstractThe mouse is an increasingly important system for the study of both normal and aberrant hematopoiesis. As a model organism, the mouse recapitulates much of human hematopoiesis; however, there are some important differences. Here, the basic approaches for analyzing hematopoiesis in mice are described. In particular, methods are provided for the collection and analysis of peripheral blood, flow cytometry analysis of peripheral blood, bone marrow, and spleen cells, and isolation and transplantation of bone marrow stem cells. Curr. Protoc. Mouse Biol. 1:323‐346 © 2011 by John Wiley & Sons, Inc.
Systematic Evaluation of Skeletal Mechanical Function
Authors: Smith Lauren , Bigelow Erin M.R. , Jepsen Karl J. 
Published: January 2015

AbstractMany genetic and environmental perturbations lead to measurable changes in bone morphology, matrix composition, and matrix organization. Here, straightforward biomechanical methods are described that can be used to determine whether a genetic or environmental perturbation affects bone strength. A systematic method is described for evaluating how bone strength is altered in the context of morphology and tissue‐level mechanical properties, which are determined in large part from matrix composition, matrix organization, and porosity. The methods described include computed tomography, whole‐bone mechanical tests (bending and compression), tissue‐level mechanical tests, and determination of ash content, water content, and bone density. This strategy is intended as a first step toward screening mice for phenotypic effects on bone and establishing the associated biomechanical mechanism by which function has been altered, and can be conducted without a background in engineering. The outcome of these analyses generally provides insight into the next set of experiments required to further connect cellular perturbation with functional change. Curr. Protoc. Mouse Biol. 3:39‐67 © 2013 by John Wiley & Sons, Inc.
Blood Collection from Mice and Hematological Analyses on Mouse Blood
Authors: Rathkolb Birgit , Fuchs Helmut , Gailus‐Durner Valérie , Aigner Bernhard , Wolf Eckhard , Hrabě de Angelis Martin 
Published: January 2015

AbstractBasic phenotyping of inbred mouse strains and genetically modified mouse models usually includes the determination of blood‐based parameters as a diagnostic screen for genotype effects on metabolism and organ function. A broad range of analytes, including hematological parameters, can be reliably determined in mouse blood, if appropriate samples are available. Here we describe recommended techniques for blood collection from mice and the considerations that have to be taken into account to get adequate samples for hematological investigations. Furthermore, we describe established methods used in the German Mouse Clinic (GMC) to determine hematological parameters in the mouse. Curr. Protoc. Mouse Biol. 3:101‐119 © 2013 by John Wiley & Sons, Inc.