bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hydrogen gas barrier property of polyelectrolyte/GO layer‐by‐layer films
Authors: Zhao Lili , Sun Haixiang , Kim Namhoon , Lee Joonghee , Kong Ying , Li Peng 
Published: January 2015

ABSTRACTDifferent types of ultrathin multilayer composite membranes adsorbed on polyethylene terephthalate (PET) substrates are fabricated by the layer‐by‐layer (LBL) self‐assembly technique. The hydrogen gas barrier performances of these membranes are measured using a pressure permeation instrument. Polyethylenimines/graphene oxide (PEI/GO) are chosen as the optimal system; the multilayer film reduces the hydrogen transmission rate of the uncoated PET film from 1357 to 24 cm3/(m2 24 h 0.1 MPa). The membrane assembly process for the PEI/GO system is analyzed with UV–Visible spectroscopy, and the flat morphology of the ultrathin film is observed by scanning electron and atomic force microscopies. Moreover, in order to fully characterize the PEI/GO multilayer film system, we investigate the effects of multiple variables on the hydrogen resistance performance. These include the molecular weight of PEI, concentrations of PEI and GO, number of bilayers, soaking time, and drying methods. The film thickness is found to increase linearly during the LBL assembly process. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41973.
The effect of an anhydride curing agent, an accelerant, and non‐ionic surfactants on the electrical resistivity of graphene/epoxy composites
Authors: Chiang Tzu Hsuan , Liu Chun‐Yu , Lin Ya‐Chun 
Published: January 2015

ABSTRACTThis study investigated different contents of an anhydride curing agent, an accelerant, and non‐ionic surfactants on the electrical resistivity of cured graphene/epoxy composites. The anhydride curing agent was hexahydrophthalic anhydride (HHPA), the accelerant was 2‐ethyl‐4‐methyl‐1H‐imidazole‐1‐propanenitrile (EMIP), and the non‐ionic surfactants were Triton X surfactants with different numbers of polyethylene oxide (PEO) groups (m) that influence the electrical resistivity of cured graphene/epoxy composites. During the curing process, differential scanning calorimetry (DSC) was used to determine the effects of the extent of the crosslinking for different contents of the curing agent and how different enthalpy (ΔH) on the electrical resistivity of the cured graphene/epoxy composites was then generated. The cured graphene/epoxy composite—which consisted of a 1 : 0.85 weight ratio of epoxy resin and anhydride, a 0.5 wt % accelerant, and a 13 wt % graphene powder—had a low electrical resistivity of 11.68 Ω·cm and a thermal conductivity of 1.7 W/m·K. In addition, the cured composites contained a 1.0 wt % polyethylene glycol p‐isooctylphenyl ether (X‐100) surfactant, which effectively decreased their electrical resistivity. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41975
miR‐129‐1‐3p Promote BGC‐823 Cell Proliferation by Targeting PDCD2
Authors: Du Yantao , Wang Danping , Luo Lin , Guo Junming 
Published: January 2015

ABSTRACTMicroRNAs (miRNAs) are the class of small noncoding RNAs, and play an important role in the regulation of gene expression at the posttranscriptional level. In this study, we explored the effect of miR‐129‐1‐3p on the growth and cell cycle of human gastric cancer cell line BGC‐823. The miR‐129‐1‐3p mimics or inhibitors were transfected into the BGC‐823 cell line, and the cell cycle and cell growth was measured by flow cytometry and real‐time cell analyzer, respectively. The possible targets of miR‐129‐1‐3p were analyzed by quantitative real time‐PCR (QRT‐PCR), Western blotting and Luciferase reporter assay. The results showed that miR‐129‐1‐3p could promote the growth and cell cycle of BGC‐823 cells. Although protein expression of programmed cell death 2 (PDCD2) was not changed with miR‐129‐1‐3p, QRT‐PCR showed that expression of PDCD2 mRNA was negatively related to the miR‐129‐1‐3p. Luciferase reporter assay revealed that PDCD2 is one of the targets of miR‐129‐1‐3p. Our results indicated that miR‐129‐1‐3p might promote proliferation of BGC‐823 cells by targeting PDCD2. Anat Rec, 297:2273–2279, 2014. © 2014 Wiley Periodicals, Inc.
Identification of urinary protein biomarkers for tobacco smoking
Authors: Haniff AJ Nabill , Gam Lay‐Harn 
Published: January 2015

AbstractSmoking, passive smoking, and nonsmoking are conditions that give different degrees of stress to the body. In this study, a proteomic technique was used to analyze differentially urinary protein expression between these three groups of subjects. Urinary proteins were precipitated using ammonium sulfate followed by separation according to molecular weights using SDS‐PAGE. The gel was stained by Coommassie blue, and the image of the gel was captured for the comparison study. The protein bands that were consistently detected but expressed at different intensity between the smokers and nonsmokers were targeted for further analysis. Three targeted protein bands were excised from the gel, consisting of a unique protein band of smokers and a pair of differentially expressed protein bands from smokers and nonsmokers. The proteins were digested in gel by trypsin. The tryptic peptides were analyzed with ultra performance liquid chromatography–tandem mass spectrometry. Protein identity was determined by the product ion spectrum in the MS/MS scan. Four unique proteins from the smokers, namely, pancreatic alpha amylase, proepidermal growth factor, protein 4.1, and prostatic acid phosphatase, were found to be potential urinary biomarkers to indicate smoking status of a person.
Isolation and characterization of novel multifunctional recombinant family 26 glycoside hydrolase from Mehsani buffalo rumen metagenome
Authors: Patel Avani B. , Patel Amrutlal K. , Shah Mihir P. , Parikh Ishan K. , Joshi Chaitanya G. 
Published: January 2015

AbstractRumen microbiota harbor a diverse set of carbohydrate‐active enzymes (CAZymes), which play a crucial role in the degradation of a complex plant polysaccharide thereby providing metabolic energy to the host animals. Earlier, we reported CAZYme analysis from the buffalo rumen metagenome by high throughput shotgun sequencing. Among the various CAZymes, glycoside hydrolase family 26 (GH26) enzymes have a number of industrial applications including in paper, oil, biofuel, food, feed, pharmaceutical, coffee, and detergent industries. Here, we report isolation and characterization of GH26 enzyme from the buffalo rumen metagenome. A novel GH26 gene composed of 1,119 base pairs was successfully amplified using the gene‐specific primers inferred based on the contig generated from metagenome sequence assembly and cloned in a pET32a (+) expression vector as an N‐terminal histidine tag fusion protein. A novel GH26 protein from an unknown rumen microorganism shared a maximum of 68% identity with the Prevotella ruminicola 23 encoded carbohydrate esterase family 7 and 46% with Bacteroides sp. 2_1_33B encoded mannan endo‐1, 4‐β‐mannosidase. The recombinant GH26‐histidine tag fusion protein was expressed in Escherichia coli and purified using Ni‐NTA affinity chromatography. The purified enzyme displayed multifunctional activities against various carbohydrate substrates including locust bean gum, beechwood xylan, pectin, and carboxymethyl cellulose suggesting mannanase, xylanase, pectin esterase, and endoglucanase activities, respectively.
Evaluation of the bioactive properties of avenanthramide analogs produced in recombinant yeast
Authors: Moglia Andrea , Goitre Luca , Gianoglio Silvia , Baldini Eva , Trapani Eliana , Genre Andrea , Scattina Antonella , Dondo Giancarlo , Trabalzini Lorenza , Beekwilder Jules , Retta Saverio Francesco 
Published: January 2015

AbstractSaccharomyces cerevisiae has been proven to be a valuable tool for the expression of plant metabolic pathways. By engineering a S. cerevisiae strain with two plant genes (4cl‐2 from tobacco and hct from globe artichoke) we previously set up a system for the production of two novel phenolic compounds, N‐(E)‐p‐coumaroyl‐3‐hydroxyanthranilic acid (Yeast avenanthramide I, Yav I) and N‐(E)‐caffeoyl‐3‐hydroxyanthranilic acid (Yeast avenanthramide II, Yav II). These compounds have a structural similarity with a class of bioactive oat compounds called avenanthramides. By developing a fermentation process for the engineered S. cerevisiae strain, we obtained a high‐yield production of Yav I and Yav II. To examine the biological relevance of these compounds, we tested their potential antioxidant and antiproliferative properties upon treatment of widely used cell models, including immortalized mouse embryonic fibroblast cell lines and HeLa cancer cells. The outcomes of our experiments showed that both Yav I and Yav II enter the cell and trigger a significant up‐regulation of master regulators of cell antioxidant responses, including the major antioxidant protein SOD2 and its transcriptional regulator FoxO1 as well as the down‐regulation of Cyclin D1. Intriguingly, these effects were also demonstrated in cellular models of the human genetic disease Cerebral Cavernous Malformation, suggesting that the novel phenolic compounds Yav I and Yav II are endowed with bioactive properties relevant to biomedical applications. Taken together, our data demonstrate the feasibility of biotechnological production of yeast avenanthramides and underline a biologically relevant antioxidant activity of these molecules. © 2015 BioFactors, 41(1):15–27, 2015
Part I: cancer in Sudan—burden, distribution, and trends breast, gynecological, and prostate cancers
Authors: Elamin Amany , Ibrahim Muntaser E. , Abuidris Dafalla , Mohamed Kamal Eldin H. , Mohammed Sulma Ibrahim 
Published: January 2015

AbstractDespite the growing burden of cancer worldwide, it continues to receive low priority in Africa, across the continent and specifically in Sudan. This is due to political unrest, limited health resources, and other pressing public health issues such as infectious diseases. Lack of awareness about the magnitude of the current and future cancer burden among policy makers play a major role as well. Although, the real scope of cancer in Sudan is not known, the reported cases have increased from 303 in 1967–6303 in 2010. According to Globocan estimates, the top most common cancers in both sexes are breast, non‐Hodgkin lymphoma, leukemia, esophagus, and colorectum. This review is the first of four papers that focuses on cancer, its distribution and trend as well as the risk factors most common in Sudan. It is expected that cancer will increase in Sudan as a result of migration of people from rural areas to urban cities in the pursuit of a better standard of living, which has resulted in lifestyle and behavioral changes that include tobacco chewing and smoking, unhealthy dieting, and a lack of physical activity. These changes are further exacerbated by the aging population and have made the country vulnerable to many diseases including cancer. These reviews are meant to provide a better understanding and knowledge required to plan appropriate cancer‐control and prevention strategies in the country.
Warburg effect regulated by amphiregulin in the development of colorectal cancer
Authors: Nam Sung Ouk , Yotsumoto Fusanori , Miyata Kohei , Fukagawa Satoshi , Yamada Hiromi , Kuroki Masahide , Miyamoto Shingo 
Published: January 2015

AbstractColorectal cancer (CRC) is one of the most frequently occurring cancers with high morbidity and mortality worldwide. Amphiregulin (AREG), a member of the epidermal growth factor family and a rational target for CRC therapy, is essential for the three‐dimensional structure of tumor formation. To clone the genes associated with increased AREG expression, we performed a cDNA microarray analysis in two CRC cell lines undergoing two‐dimensional (2DC) and three‐dimensional culture (3DC). Upregulated (>2.0‐fold) and downregulated (<0.5‐fold) genes in 3DC compared with 2DC were selected. Pathway analysis using DAVID based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway databases revealed a number of genes involved in glycolysis. In CRC cells, glucose elevated the expression of GLUT1 and AREG as well as the activity of the hypoxia‐inducible factor 1 (HIF‐1) luciferase reporter promoter. The suppression of AREG expression reduced the uptake of glucose and production of lactate. Luciferase assay identified a critical regulatory region for AREG expression between −130 and −180 bp upstream of the start site, which contained a carbohydrate response element (ChoRE). Max‐like protein X (MLX) bound to ChoRE and enhanced the expression of AREG. Together these data suggest that AREG plays a pivotal role in the development of CRC through activation of the Warburg effect.
FilGAP, a Rac‐specific Rho GTPase‐activating protein, is a novel prognostic factor for follicular lymphoma
Authors: Nishi Tatsuya , Takahashi Hiroyuki , Hashimura Miki , Yoshida Tsutomu , Ohta Yasutaka , Saegusa Makoto 
Published: January 2015

AbstractFilGAP, a Rho GTPase‐activating protein (GAP), acts as a mediator of Rho/ROCK (Rho‐associated protein kinase)‐dependent amoeboid movement, and its knockdown results in Rac‐driven mesenchymal morphology. Herein, we focus on the possible roles of FilGAP expression in normal and malignant lymphocytes. Eighty‐three cases of follicular lymphoma (FL), 84 of diffuse large B‐cell lymphoma (DLBCL), and 25 of peripheral T‐cell lymphoma (PTCL), as well as 10 of normal lymph nodes, were immunohistochemically investigated. In normal lymph nodes, FilGAP immunoreactivity was significantly higher in lymphocytes in the mantle zone as compared to those in the germinal center and paracortical areas. In contrast, the expression levels of both cytoplasmic and perinuclear Rac1 were significantly lower in the germinal center as compared to paracortical regions, suggesting that changes in the FilGAP/Rac axis may occur in B‐cell lineages. In malignant lymphomas, FilGAP expression was significantly higher in B‐cell lymphomas than PTCL, and the immunohistochemical scores were positively correlated with cytoplasmic Rac1 scores in FL and DLBCL, but not in PTCL. Patients with FL and germinal center B‐cell‐like (GCB)‐type DLBCL showing high FilGAP scores had poor overall survival rates as compared to the low‐score patients. Moreover, multivariate Cox regression analysis showed that a high FilGAP score was a significant and independent unfavorable prognostic factor in FL, but not in DLBCL. In conclusion, FilGAP may contribute to change in cell motility of B‐lymphocytes. In addition, its expression appears to be useful for predicting the behavior of B‐cell lymphoma, in particular FL.
HIV infection and domestic smoke exposure, but not human papillomavirus, are risk factors for esophageal squamous cell carcinoma in Zambia: a case–control study
Authors: Kayamba Violet , Bateman Allen C. , Asombang Akwi W. , Shibemba Aaron , Zyambo Kanekwa , Banda Themba , Soko Rose , Kelly Paul 
Published: January 2015

AbstractThere is emerging evidence that esophageal cancer occurs in younger adults in sub‐Saharan Africa than in Europe or North America. The burden of human immunodeficiency virus (HIV) is also high in this region. We postulated that HIV and human papillomavirus (HPV) infections might contribute to esophageal squamous cell carcinoma (OSCC) risk. This was a case–control study based at the University Teaching Hospital in Lusaka, Zambia. Cases were patients with confirmed OSCC and controls had completely normal upper endoscopic evaluations. A total of 222 patients were included to analyze the influence of HIV infection; of these, 100 patients were used to analyze the influence of HPV infection, alcohol, smoking, and exposure to wood smoke. The presence of HIV infection was determined using antibody kits, and HPV infection was detected by polymerase chain reaction. HIV infection on its own conferred increased risk of developing OSCC (odds ratio [OR] 2.3; 95% confidence interval [CI] 1.0–5.1; P = 0.03). The OR was stronger when only people under 60 years were included (OR 4.3; 95% CI 1.5–13.2; P = 0.003). Cooking with charcoal or firewood, and cigarette smoking, both increased the odds of developing OSCC ([OR 3.5; 95% CI 1.4–9.3; P = 0.004] and [OR 9.1; 95% CI 3.0–30.4; P < 0.001], respectively). There was no significant difference in HPV detection or alcohol intake between cases and controls. We conclude that HIV infection and exposure to domestic and cigarette smoke are risk factors for OSCC, and HPV immunization unlikely to reduce OSCC incidence in Zambia.
Establishing the Stability and Reversibility of Protein Pyrophosphorylation with Synthetic Peptides
Authors: Yates Lisa M. , Fiedler Dorothea 
Published: January 2015

AbstractProtein pyrophosphorylation is emerging as a new post‐translational modification, yet its role in cellular signaling remains poorly characterized. Important factors in determining the biological relevance of pyrophosphorylation include understanding the chemical and biochemical stability of the pyrophosphoryl group and elucidating the reversibility of modification in a cellular context. Towards this end, we prepared a series of synthetic pyrophosphopeptides, which were utilized to demonstrate that the modification is quite inert over a wide pH range but can be removed biochemically by alkaline phosphatases. Importantly, we observed enzyme‐dependent removal of the pyrophosphate in mammalian and yeast cell lysates using the synthetic pyrophosphopeptides. The findings provide evidence for the reversibility of pyrophosphorylation and thereby highlight the potential impact of this modification on cellular signal transduction pathways.
Prognostic utility of myocardial blush grade after PCI in patients with NSTE‐ACS: Analysis from the ACUITY trial
Authors: Ng Vivian G. , Lansky Alexandra J. , Toro Saleem , Parise Helen , Cristea Ecaterina , Mehran Roxana , Stone Gregg W. 
Published: January 2015

ObjectiveWe evaluated the ability of post‐procedural myocardial blush grade (MBG) to stratify outcomes of patients undergoing percutaneous coronary intervention (PCI) for non‐ST segment elevation acute coronary syndromes (NSTE‐ACS).BackgroundMBG strongly correlates with survival after reperfusion therapy in patients with ST‐segment elevation myocardial infarction (STEMI).MethodsOf 13,819 NSTE‐ACS patients randomized in the ACUITY trial, 3,115 patients underwent PCI and had MBG analyzed by an independent angiographic core laboratory. We examined net adverse clinical events (NACE; composite ischemia or bleeding), composite ischemia (death, MI or ischemia‐driven revascularization) and non‐CABG major bleeding according to final MBG.ResultsAt 30 days, patients with MBG‐0/1 had higher rates of NACE (25.1% vs. 13.9%, P = 0.002) and composite ischemia (19.1% vs. 9.4%, P = 0.002) than patients with MBG‐2/3. At 1‐year follow‐up, MBG‐0/1 patients had significantly higher rates of composite ischemia compared to other patients (27.8% vs. 19.8%, P = 0.02). By multivariable analysis, MBG‐0/1 was an independent predictor of 30‐day ischemia‐driven revascularization (OR 5.74 [2.63, 12.54], P < 0.0001) in the total population and among patients with normal post‐PCI epicardial TIMI‐3 flow (OR 6.39 [2.06, 19.78], P = 0.001). However, 1‐year outcomes were similar between patients with and without normal myocardial perfusion.ConclusionsIn conclusion, MBG is a predictor of 30‐day revascularization in the overall population and in patients with normal epicardial flow but fails to stratify 1‐year outcomes. Thus, unlike in STEMI patients, the prognostic value of MBG in NSTE‐ACS patients appears to be limited to the short‐term. © 2015 Wiley Periodicals, Inc.
A comparison of transcatheter aortic valve implantation and surgical aortic valve replacement in 1,141 patients with severe symptomatic aortic stenosis and less than high risk
Authors: Schymik Gerhard , Heimeshoff Martin , Bramlage Peter , Herbinger Tanja , Würth Alexander , Pilz Lothar , Schymik Jan S. , Wondraschek Rainer , Süselbeck Tim , Gerhardus Jan , Luik Armin , Gonska Bernd‐Dieter , Tzamalis Panagiotis , Posival Herbert , Schmitt Claus , Schröfel Holger 
Published: January 2015

ObjectivesTo assess outcomes for patients undergoing transcatheter aortic valve implantation (TAVI) versus surgical aortic valve replacement but with less than high risk.BackgroundWhile there is abundant data for high risk patients there is insufficient data for reduced risk.MethodsPatients undergoing TAVI or SAVR between 2007 and 2012 in Karlsruhe were considered. They were assessed by cardiac computed tomography, transoesophageal echocardiogram, and logistic EuroSCORE I (ES) and groups compared using Propensity Score Matching.ResultsThe mean ES was 10.1±2.8 in the TAVI group (n = 419) and 5.7 ± 3.2 in the SAVR group (n = 722;P < 0.0001). Mean survival probability over 3 years was higher in patients undergoing surgery (P < 0.0001). A total of 432 patients were considered for the matched‐pairs analysis based on propensity scores (216 in each group). Major vascular complications (10.6% vs. 0.0%;P < 0.0001), new pacemaker implantation (13.9% vs. 4.6%;P < 0.001) and moderate aortic insufficiency (3.2% vs. 0.5%;P = 0.03) were more frequent in patients undergoing TAVI. Major (20.8% vs. 4.2%;P < 0.0001) and life‐threatening (14.5% vs. 2.3%;P < 0.0001) bleeding complications were more frequent in those undergoing surgery. Survival probability over 3 years in the propensity matched cohort was comparable between both groups (P = 0.16).ConclusionsIn this large, single center, real world dataset there was no difference in mortality between patients undergoing TAVI or SAVR during a 3‐year follow‐up but there was a TAVI related increase in major vascular complications, new pacemaker implantation and aortic insufficiency and a SAVR related increased bleeding risk. © 2015 Wiley Periodicals, Inc.
Impact of postoperative acute kidney injury on clinical outcomes after transcatheter aortic valve implantation: A meta‐analysis of 5,971 patients
Authors: Gargiulo Giuseppe , Sannino Anna , Capodanno Davide , Perrino Cinzia , Capranzano Piera , Barbanti Marco , Stabile Eugenio , Trimarco Bruno , Tamburino Corrado , Esposito Giovanni 
Published: January 2015

BackgroundThere is conflicting evidence on the safety and efficacy of transcatheter aortic valve implantation (TAVI) in patients with postoperative acute kidney injury (AKI). Therefore, we conducted a meta‐analysis on the impact of AKI on clinical outcomes after TAVI.Methods and ResultsTwenty‐four studies including 5,971 patients were analyzed. The mean incidence of AKI in this population was 22.1% ± 11.2. Postoperative AKI significantly increased early (odds ratio [OR] 5.09; 95% confidence interval [CI], 4.03–6.43 and OR 6.14; 95% CI, 3.26–11.55) and 1‐year (OR 3.27; 95% CI, 2.42–4.42 and OR 1.93; 95% CI, 1.38–2.71) all‐cause and cardiovascular mortality respectively, but also early myocardial infarction (OR 3.30; 95% CI, 1.44–7.57), life‐threatening bleeding (OR 2.90; 95% CI, 1.67–5.05), need for transfusion (OR 2.42; 95% CI, 1.96–2.99), and dialysis (OR 14.35; 95% CI, 6.21–33.20), with a non‐significant increase of stroke (OR 1.66; 95% CI, 0.94–2.95), hospitalization (mean difference [MD] 1.73; 95% CI, −0.31 to 3.77) and contrast medium received (MD 4.74; 95% CI, −2.33 to 11.81).ConclusionsPostoperative AKI seems to significantly worsen TAVI prognosis. The results of the present meta‐analysis should be considered hypothesis‐generating and future studies on risk stratification, prevention, and postoperative management are needed. © 2015 Wiley Periodicals, Inc.