bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adipose‐Derived Mesenchymal Stem Cells Exert Antiinflammatory Effects on Chondrocytes and Synoviocytes From Osteoarthritis Patients Through Prostaglandin E2
Authors: Manferdini Cristina , Maumus Marie , Gabusi Elena , Piacentini Anna , Filardo Giuseppe , Peyrafitte Julie‐Anne , Jorgensen Christian , Bourin Philippe , Fleury‐Cappellesso Sandrine , Facchini Andrea , Noël Danièle , Lisignoli Gina 
Published: February 2013

AbstractObjectiveTo examine the effect of different sources of Good Manufacturing Practice clinical grade adipose‐derived mesenchymal stem cells (AD‐MSCs) on inflammatory factors in osteoarthritic (OA) chondrocytes and synoviocytes.MethodsAD‐MSCs from infrapatellar Hoffa fat, subcutaneous (SC) hip fat, and SC abdominal fat were cocultured in Transwells with chondrocytes or synoviocytes. Inflammatory factors (interleukin‐1β [IL‐1β], tumor necrosis factor α, IL‐6, CXCL1/growth‐related oncogene α, CXCL8/IL‐8, CCL2/monocyte chemotactic protein 1, CCL3/macrophage inflammatory protein 1α, and CCL5/RANTES) were evaluated by quantitative reverse transcription–polymerase chain reaction or multiplex bead–based immunoassay. The role of different immunomodulators was analyzed.ResultsAll the inflammatory factors analyzed were down‐modulated at the messenger RNA or protein level independently by all 3 AD‐MSC sources or by allogeneic AD‐MSCs used in coculture with chondrocytes or synoviocytes. Inflammatory factor down‐modulation was observed only when AD‐MSCs were cocultured with chondrocytes or synoviocytes that produced high levels of inflammatory factors, but no effect was observed in cells that produced low levels of those factors, thus highlighting a dependence of the AD‐MSC effect on existing inflammation. The immunomodulators IL‐10, IL‐1 receptor antagonist, fibroblast growth factor 2, indoleamine 2,3‐dioxygenase 1, and galectin 1 were not involved in AD‐MSC effects, whereas the cyclooxygenase 2 (COX‐2)/prostaglandin E2 (PGE2) pathway exerted a role in the mechanism of antiinflammatory AD‐MSC action.ConclusionThe antiinflammatory effects of AD‐MSCs are probably not dependent on AD‐MSC adipose tissue sources and donors but rather on the inflammatory status of OA chondrocytes and synoviocytes. AD‐MSCs seem to be able to sense and respond to the local environment. Even though a combination of different molecules may be involved in AD‐MSC effects, the COX‐2/PGE2 pathway may play a role, suggesting that AD‐MSCs may be useful for therapies in osteoarticular diseases.
Development and validation of a spectrofluorimetric method for the quantification of ceftriaxone in pharmaceutical formulations and plasma
Authors: Shah Jasmin , Jan M. Rasul , Shah Sultan , Inayatullah  
Published: February 2013

ABSTRACTWe describe the development and validation of a new, simple, sensitive and cost‐effective method for the determination of ceftriaxone in commercial formulations and spiked human plasma. The method proposes the conversion of ceftriaxone into a fluorescent product by reacting with ortho‐phthalaldehyde (OPA) in the presence of sulfite at room temperature. The reaction medium is buffered to pH 10 using borate buffer. The derivatized reaction product is highly fluorescent and exhibits maximum fluorescence intensity at λem = 386 nm after excitation at λex = 324 nm. The experimental parameters affecting progress of the derivatization reaction were carefully studied and optimized. Under optimum experimental conditions, the method has an excellent correlation coefficient of 0.9984 with a broad linear range of 0.4−20 µg/mL. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 1.30 × 10−3 and 3.90 × 10−3 µg/mL, respectively. The interference effects of common excipients on the quantification of drug were investigated and no interference effect was observed. The proposed method has been successfully applied to the determination of ceftriaxone in pharmaceutical formulations and spiked human plasma samples. The method has been validated statistically through percent recovery studies using standard addition and by comparison with a reference HPLC method. The developed method exhibits excellent inter‐ and intraday precision. Copyright © 2013 John Wiley & Sons, Ltd.
Long‐term clinical outcomes after percutaneous coronary intervention for chronic total occlusions in elderly patients (≥75 Years)
Authors: Hoebers Loes P. , Claessen Bimmer E. , Dangas George D. , Park Seung‐Jung , Colombo Antonio , Moses Jeffrey W. , Henriques José P.S. , Stone Gregg W. , Leon Martin B. , Mehran Roxana ,   the Multinational CTO Registry
Published: February 2013

ObjectiveTo investigate procedural success rates and long‐term clinical outcome of percutaneous coronary intervention (PCI) for chronic total occlusions (CTO) in elderly patients.BackgroundLittle is known about procedural success and long‐term clinical outcome of PCI for CTO in the elderly.MethodsA total of 1,791 consecutive patients with 1,852 CTO underwent PCI at three large centers in USA, Italy, and South Korea. Outcomes included procedural success and major adverse cardiac events (MACE, composite of mortality, myocardial infarction, or coronary artery bypass graft surgery [CABG]).Time‐to‐event analyses were performed using Kaplan−Meier statistics, and the log‐rank statistic was used to test for differences between patients aged ≥75 and patients aged <75 years.ResultsTwo hundred and thirteen patients (12%) were aged ≥75 years. Procedural success rates were similar in elderly patients compared with patients <75 years (63.8% vs. 69.1%, P = 0.12). Median follow‐up was 890 days (IQR: 380–1,480 days). MACE rates after successful versus failed PCI were 25.8% versus 42.3% in the elderly (P = 0.02) and 11.2 versus 20.8% in younger patients (P < 0.01). In elderly patients, this reduction in MACE after successful PCI was mainly driven by a reduction in CABG (0.0% vs. 20.4%, P < 0.01), there were no significant differences in terms of mortality (19.6% vs. 24.6%, P = 0.13) or MI (11.5% vs. 8.0%, P = 0.87).ConclusionCTO PCI in patients ≥75 years has similar success as in patients <75 years. In elderly patients undergoing CTO PCI, MACE rates were relatively high but successful revascularization is associated with a reduction in MACE at 5‐year follow‐up in both elderly and younger patients. © 2013 Wiley Periodicals, Inc.
A Combination Strategy to Inhibit Pim‐1: Synergism between Noncompetitive and ATP‐Competitive Inhibitors
Authors: Mori Mattia , Tintori Cristina , Christopher Robert Selwyne Arul , Radi Marco , Schenone Silvia , Musumeci Francesca , Brullo Chiara , Sanità Patrizia , Delle Monache Simona , Angelucci Adriano , Kissova Miroslava , Crespan Emmanuele , Maga Giovanni , Botta Maurizio 
Published: February 2013

AbstractPim‐1 is a serine/threonine kinase critically involved in the initiation and progression of various types of cancer, especially leukemia, lymphomas and solid tumors such as prostate, pancreas and colon, and is considered a potential drug target against these malignancies. In an effort to discover new potent Pim‐1 inhibitors, a previously identified ATP‐competitive indolyl‐pyrrolone scaffold was expanded to derive structure–activity relationship data. A virtual screening campaign was also performed, which led to the discovery of additional ATP‐competitive inhibitors as well as a series of 2‐aminothiazole derivatives, which are noncompetitive with respect to both ATP and peptide substrate. This mechanism of action, which resembles allosteric inhibition, has not previously been characterized for Pim‐1. Notably, further evaluation of the 2‐aminothiazoles indicated a synergistic inhibitory effect in enzymatic assays when tested in combination with ATP‐competitive inhibitors. A synergistic effect in the inhibition of cell proliferation by ATP‐competitive and ATP‐noncompetitive compounds was also observed in prostate cancer cell lines (PC3), where all Pim‐1 inhibitors tested in showed synergism with the known anticancer agent, paclitaxel. These results further establish Pim‐1 as a target in cancer therapy, and highlight the potential of these agents for use as adjuvant agents in the treatment of cancer diseases in which Pim‐1 is associated with chemotherapeutic resistance.
Intrinsic horizontal connections process global tactile features in the primary somatosensory cortex: Neuroanatomical evidence
Authors: Négyessy László , Pálfi Emese , Ashaber Mária , Palmer Cory , Jákli Balázs , Friedman Robert M. , Chen Li M. , Roe Anna W. 
Published: February 2013

ABSTRACTTo understand manual tactile functions in primates, it is essential to explore the interactions between the finger pad representations in somatosensory cortex. To this end, we used optical imaging and electrophysiological mapping to guide neuroanatomical tracer injections into distal digit tip representations of Brodmann area 3b in the squirrel monkey. Retrogradely labeled cell densities and anterogradely labeled fibers and terminal patches in somatosensory areas were plotted and quantified with respect to tangential distribution. Within area 3b, reciprocal patchy distribution of anterograde and retrograde labeling spanned the representation of the distal pad of multiple digits, indicating strong cross‐digit connectivity. Inter‐areal connections revealed bundles of long‐range fibers projecting anteroposteriorly, connecting area 3b with clusters of labeled neurons and terminal axon arborizations in area 1. Inter‐areal linkage appeared to be largely confined to the representation of the injected finger. These findings provide the neuroanatomical basis for the interaction between distal finger pad representations observed by recent electrophysiological studies. We propose that intra‐areal connectivity may be heavily involved in interdigit integration such as shape discrimination, whereas long‐range inter‐areal connections may subserve active touch in a digit‐specific manner. J. Comp. Neurol. 521:2798–2817, 2013. © 2013 Wiley Periodicals, Inc.
Chronic neurotrophin delivery promotes ectopic neurite growth from the spiral ganglion of deafened cochleae without compromising the spatial selectivity of cochlear implants
Authors: Landry Thomas G. , Fallon James B. , Wise Andrew K. , Shepherd Robert K. 
Published: February 2013

ABSTRACTCochlear implants restore hearing cues in the severe–profoundly deaf by electrically stimulating spiral ganglion neurons (SGNs). However, SGNs degenerate following loss of cochlear hair cells, due at least in part to a reduction in the endogenous neurotrophin (NT) supply, normally provided by hair cells and supporting cells of the organ of Corti. Delivering exogenous NTs to the cochlea can rescue SGNs from degeneration and can also promote the ectopic growth of SGN neurites. This resprouting may disrupt the cochleotopic organization upon which cochlear implants rely to impart pitch cues. Using retrograde labeling and confocal imaging of SGNs, we determined the extent of neurite growth following 28 days of exogenous NT treatment in deafened guinea pigs with and without chronic electrical stimulation (ES). On completion of this treatment, we measured the spread of neural activation to intracochlear ES by recording neural responses across the cochleotopically organized inferior colliculus using multichannel recording techniques. Although NT treatment significantly increased both the length and the lateral extent of growth of neurites along the cochlea compared with deafened controls, these anatomical changes did not affect the spread of neural activation when examined immediately after 28 days of NT treatment. NT treatment did, however, result in lower excitation thresholds compared with deafened controls. These data support the application of NTs for improved clinical outcomes for cochlear implant patients. J. Comp. Neurol. 521:2818–2832, 2013. © 2013 Wiley Periodicals, Inc.
Grid‐based molecular footprint comparison method for docking and de novo design: Application to HIVgp41
Authors: Balius Trent E. , Allen William J. , Mukherjee Sudipto , Rizzo Robert C. 
Published: February 2013

AbstractScoring functions are a critically important component of computer‐aided screening methods for the identification of lead compounds during early stages of drug discovery. Here, we present a new multigrid implementation of the footprint similarity (FPS) scoring function that was recently developed in our laboratory which has proven useful for identification of compounds which bind to a protein on a per‐residue basis in a way that resembles a known reference. The grid‐based FPS method is much faster than its Cartesian‐space counterpart, which makes it computationally tractable for on‐the‐fly docking, virtual screening, or de novo design. In this work, we establish that: (i) relatively few grids can be used to accurately approximate Cartesian space footprint similarity, (ii) the method yields improved success over the standard DOCK energy function for pose identification across a large test set of experimental co‐crystal structures, for crossdocking, and for database enrichment, and (iii) grid‐based FPS scoring can be used to tailor construction of new molecules to have specific properties, as demonstrated in a series of test cases targeting the viral protein HIVgp41. The method is available in the program DOCK6. © 2013 Wiley Periodicals, Inc.
Effect of chemical modifications on the electronic structure of poly(3‐hexylthiophene)
Authors: Oliveira Eliézer Fernando de , Camilo‐Jr Alexandre , Silva‐Filho Luiz Carlos da , Lavarda Francisco Carlos 
Published: February 2013

AbstractThe widespread use of poly(3‐hexylthiophene) (P3HT) in the active layers of organic solar cells indicates that it possesses chemical stability and solubility suitable for such an application. However, it would be desirable to have a material that can maintain these properties but with a smaller bandgap, which would lead to more efficient energy harvesting of the solar spectrum. Fifteen P3HT derivatives were studied using the Density Functional Theory. The conclusion is that it is possible to obtain compounds with significantly smaller bandgaps and with solubility and stability similar to that of P3HT, mostly through the binding of oxygen atoms or conjugated organic groups to the thiophenic ring. © 2013 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys., 2013
Karydakis Flap for Recurrent Pilonidal Disease
Authors: Iesalnieks Igors , Deimel Sina , Schlitt Hans J. 
Published: February 2013

AbstractBackgroundPatients undergoing surgery for recurrent pilonidal disease are at high risk of developing re‐recurrence. The present retrospective analysis was performed to compare long‐term results in patients with recurrent disease undergoing midline excision surgery compared to patients undergoing the Karydakis flap procedure.MethodsOnly patients with previous excision surgery apart from simple abscess incision were included. Disease recurrence was defined as the need for repeat surgery.ResultsA total of 124 patients underwent surgery for recurrent pilonidal disease. Group 1 consisted of 37 patients (25 excision + midline closure, 12 excision + lay‐open). Group 2 consisted of 87 patients (Karydakis flap). There were no statistically significant differences between the groups with regard to patient’s age, duration of disease, body mass index, or sex. The average number of previous surgeries was significantly higher in group 1 patients (2.1 vs. 1.8, p = 0.019). The overall 1‐year recurrence rate was 43 % in group 1 and 3 % in group 2 (p < 0.0001). The wound dehiscence rate after the Karydakis flap procedure was as high as 43 % between years 2005 and 2009, but it fell to 10 % thereafter (p = 0.02).ConclusionsKarydakis flap procedure is superior to midline excision surgery in patients presenting with recurrent pilonidal disease.
Bilateral Anterior Thoracotomy (Clamshell Incision) Is the Ideal Emergency Thoracotomy Incision: An Anatomic Study
Authors: Simms Eric R. , Flaris Alexandros N. , Franchino Xavier , Thomas Michael S. , Caillot Jean‐Louis , Voiglio Eric J. 
Published: February 2013

AbstractBackgroundEmergency thoracotomy (ET) is a procedure that provides rapid access to intrathoracic structures for thoracic trauma patients arriving at the hospital in extremis. This study assesses the accessibility of intrathoracic structures provided by six different ET incisions. We hypothesize that the bilateral anterior thoracotomy (“clamshell” incision) provides the most rapid and definitive accessibility to intrathoracic structures.MethodsSix ET incision types (left anterolateral thoracotomy, right anterolateral thoracotomy, left 2nd intercostal space incision, left 3rd intercostal space incision, median sternotomy, and bilateral anterior thoracotomy) were performed multiple times on eight cadavers. The critical intrathoracic structures were assessed for rapid accessibility and control, and they were characterized as “readily accessible,” “accessible,” and “inaccessible” on anatomic accessibility maps.ResultsMedian sternotomy provided better access to intrathoracic structures than left and right anterior thoracotomies. Definitive control of the origin of the left subclavian artery was difficult with left 2nd or 3rd intercostal space incisions. Bilateral anterior thoracotomy, the clamshell incision, was easy to perform and gave superior access to all intrathoracic structures.ConclusionsIn severe thoracic trauma, specific injuries are unknown, even if they can be anticipated. The best incision is therefore one that provides the most rapid and definitive access to all thoracic structures for assessment and control. While the right and left anterolateral incisions may be successfully employed by surgeons with extensive experience in ET, the clamshell incision remains the superior incision choice.Level of Evidence IIObservational study.
Weight status and dietary intake determine serum leptin concentrations in pregnant and lactating women and their infants
Authors: Vähämiko Sanna , Isolauri Erika , Laitinen Kirsi 
Published: February 2013

Leptin regulates energy homeostasis and immune and metabolic functions. Highly elevated leptin concentrations during pregnancy may be associated with aberrations in maternal metabolism and long-term health consequences both in women and children. The objective of the present study was to evaluate whether dietary counselling, probiotic supplementation, maternal characteristics or dietary intake during pregnancy has an impact on serum leptin concentrations in women, cord blood or in children. A total of 256 pregnant women were randomised to a control group (n 85) or to receive dietary counselling with probiotics (n 85) or placebo (n 86). Dietary counselling aimed at affecting the type of fat used and to increase the amount of fibre in the women's diet. Women's dietary intake and serum leptin concentrations were analysed at the first and third trimesters of pregnancy and at 1 month postpartum. Furthermore, leptin concentrations were measured from the cord blood and from children's serum at 1 and 6 months of age. Weight status and dietary composition were the key determinants of leptin concentrations. Specifically, high dietary fibre and low SFA intakes were related to low serum leptin concentrations in women. Female sex and birth weight were associated with higher infant leptin, whereas cord blood leptin was additionally affected by maternal leptin concentration and protein intake. Probiotics or dietary counselling did not affect leptin concentrations. Weight control to recommended levels and modification of diet towards higher fibre and lower SFA intakes during pregnancy may through leptin concentrations provide health benefits to both women and children.
Rethinking property rights: comparative analysis of conservation easements for wildlife conservation
Authors: RISSMAN ADENA R. 
Published: February 2013

SUMMARYConservation easements (or conservation covenants) are commonly conceptualized as acquisitions of sticks in a ‘bundle of rights’ and are increasingly implemented for wildlife conservation on private lands. This research asks: (1) What are the possibilities and limitations of the conservation easement approach to wildlife conservation in contrasting rural and periurban regions? and (2) How does analysis of conservation easements differ when examining property as a bundle of rights or alternative metaphors? These questions were addressed through document analysis, interviews and GIS mapping in two regions where The Nature Conservancy deployed conservation easements for wildlife habitat: rural Lassen Foothills and periurban Tenaja Corridor, USA. Splitting the bundle allowed for site and region-specific easements with differences in permitted housing densities, land management and hunting. Easements focused on restricted rights rather than affirmative duties. The challenges of habitat connectivity in the fragmented Tenaja Corridor revealed the limits of parcel-based acquisition. Analysts and conservation practitioners should rethink the bundle of rights concept of property, considering a bundle of duties, powers and owners within a broader web of social and ecological interests, to understand the role of conservation acquisitions in contrasting landscape contexts.
Cell cycle and lineage progression of neural progenitors in the ventricular-subventricular zones of adult mice
Authors: Ponti Giovanna , Obernier Kirsten , Guinto Cristina , Jose Lingu , Bonfanti Luca , Alvarez-Buylla Arturo 
Published: February 2013

Significance
The time taken by neural stem cells and intermediate progenitor cells to transit through the cell cycle, and number of times they divide, is essential information to understand how new neurons are produced in the adult rodent brain.
Widespread Long Noncoding RNAs as Endogenous Target Mimics for MicroRNAs in Plants
Authors: Wu Hua-Jun , Wang Zhi-Min , Wang Meng , Wang Xiu-Jie 
Published: February 2013

Abstract
Target mimicry is a recently identified regulatory mechanism for microRNA (miRNA) functions in plants in which the decoy RNAs bind to miRNAs via complementary sequences and therefore block the interaction between miRNAs and their authentic targets. Both endogenous decoy RNAs (miRNA target mimics) and engineered artificial RNAs can induce target mimicry effects. Yet until now, only the Induced by Phosphate Starvation1 RNA has been proven to be a functional endogenous microRNA target mimic (eTM). In this work, we developed a computational method and systematically identified intergenic or noncoding gene-originated eTMs for 20 conserved miRNAs in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa). The predicted miRNA binding sites were well conserved among eTMs of the same miRNA, whereas sequences outside of the binding sites varied a lot. We proved that the eTMs of miR160 and miR166 are functional target mimics and identified their roles in the regulation of plant development. The effectiveness of eTMs for three other miRNAs was also confirmed by transient agroinfiltration assay.
Study of Distal Pyroclastic‐flow Stratum from Tianchi Volcano in 1215 (±15) Eruption: Pyroclastic‐flow Over Water
Authors: Bo ZHAO , Jiandong XU , Chuanyong LIN 
Published: February 2013

AbstractIn this paper, we describe three strata at the distal part of the pyroclastic‐flow from the Tianchi volcano in 1215 (±15) eruption. One of the strata with crosslayers that are different from typical pyroclastic‐flow strata may come from a ground‐surge. The grain‐size and scanning electron microscopy (SEM) analysis was performed to study the origin of the pyroclastic‐flow. Characteristics of grain‐size distribution show that it is similar with the ash cloud. Through the SEM analyses, we found some quench structures with less damage on the surfaces of the vitric pumices. These phenomena indicate that there has been hydration in the transportation processes at the distal of pyroclastic‐flow. It has partly changed the transportation mechanism of pyroclastic‐flow, which transitions form dense flow to diluted flow. This paper develops a new distal pyroclastic‐flow model in the Tianchi volcano that can be divided into three stages, i.e. the quench stage, expanding stage and depositing stage.