bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do circadian genes and ambient temperature affect substrate-borne signalling duringDrosophilacourtship?
Authors: Medina Izarne , Casal José , Fabre Caroline C. G. 
Published: October 2015

ABSTRACTCourtship vibratory signals can be air-borne or substrate-borne. They convey distinct and species-specific information from one individual to its prospective partner. Here, we study the substrate-borne vibratory signals generated by the abdominal quivers of the Drosophila male during courtship; these vibrations travel through the ground towards courted females and coincide with female immobility. It is not known which physical parameters of the vibrations encode the information that is received by the females and induces them to pause. We examined the intervals between each vibratory pulse, a feature that was reported to carry information for animal communication. We were unable to find evidence of periodic variations in the lengths of these intervals, as has been reported for fly acoustical signals. Because it was suggested that the genes involved in the circadian clock may also regulate shorter rhythms, we search for effects of period on the interval lengths. Males that are mutant for the period gene produced vibrations with significantly altered interpulse intervals; also, treating wild type males with constant light results in similar alterations to the interpulse intervals. Our results suggest that both the clock and light/dark cycles have input into the interpulse intervals of these vibrations. We wondered if we could alter the interpulse intervals by other means, and found that ambient temperature also had a strong effect. However, behavioural analysis suggests that only extreme ambient temperatures can affect the strong correlation between female immobility and substrate-borne vibrations.
Symptom burden and functional gains in a cancer rehabilitation unit
Authors: Fu Jack B , Lee Jay , Tran Kenny B , Siangco Christian M , Ng Amy H , Smith Dennis W , Bruera Eduardo 
Published: November 2015

Aim: To determine whether symptom burden at the time of admission to inpatient rehabilitation is predictive of functional improvement in cancer rehabilitation inpatients. Methods: Retrospective review of medical records at a tertiary referral-based cancer centre of all patients admitted to an inpatient rehabilitation unit between 1 March 2013 and 20 May 2013. The medical records for 71 unique cancer rehabilitation inpatients were analysed. Outcome measures included the Edmonton Symptom Assessment System and Functional Independence Measure. Results: Statistical analysis of total admission Edmonton Symptom Assessment System scores and total change in Functional Independence Measure scores found no significant relationships. The symptom burden of patients was mild. Patients demonstrated statistically significant improvements in symptoms and function during inpatient rehabilitation. The mean change in total Edmonton Symptom Assessment System and total Functional Independence Measure scores was a decrease of 7.41 and an increase of 19.20, respectively. Statistically significant changes occurred in fatigue, sleep, pain and anxiety. Conclusions: Both symptom and functional scores improved significantly during inpatient rehabilitation. However, no significant relationships were found between symptoms at admission and improvements in Functional Independence Measure score.
Patient adherence to physiotherapist prescribed self-management strategies: A critical review
Authors: Peek Kerry , Sanson-Fisher Robert , Mackenzie Lisa , Carey Mariko 
Published: November 2015

Aims: To examine the published literature on patient adherence to physiotherapist prescribed self-management strategies in order to describe: changes in the proportion of publications over time; methodological quality of non-intervention and intervention-based studies; types of measures used to assess patient adherence and the reported accuracy of those measures. Methods: A comprehensive search of eight electronic databases was conducted, covering the period from January 1995 to November 2014. Data were extracted and coded for the number and proportion of articles that were: i) non data-based; ii) data-based, no new data (reviews); iii) data-based, new data. The methodological quality of non-intervention and intervention publications was assessed using the Effective Public Health Practice Project Quality Assessment Tool, and data were extracted regarding the type and accuracy of adherence measures reported in these publications. Results: A total of 80 relevant papers were identified. Of these, 49 non-intervention and intervention quantitative study designs underwent methodological assessment, with only 14 studies (29%) assessed as being of at least moderate quality. Fifty-three different measures of patient adherence were recorded from the 49 included studies, with only 5 of the 49 included studies (10%) reporting statistical evidence to support accuracy of the adherence measures applied. Conclusions: The results indicate that despite a trend towards intervention-based studies and reviews over the last 20 years, the methodological quality of studies on patient adherence could be improved. Accurate and standardised measures of patient adherence are needed for any future research involving patient adherence to physiotherapist prescribed self-management strategies.
New Criteria to Assess Seismic and Rock Burst Hazard in Coal Mines / Nowe Kryteria Dla Oceny Zagrożenia Sejsmicznego I Tąpaniami W Kopalniach Węgla Kamiennego
Authors: Mutke Grzegorz , Dubiński Józef , Lurka Adam 
Published: November 2015

Abstract
The paper presents new criteria of seismic and rock burst hazard assessment in Polish hard coal mines where longwall mining system is common practice. The presented criteria are based on the results of continuous recording of seismic events and analysis of selected seismological parameters: spatial location of seismic event in relation to mining workings, seismic energy, seismic energy release per unit coal face advance, b-value of Gutenberg-Richter law, seismic energy index EI, seismic moment M0, weighted value of peak particle velocity PPVW. These parameters are determined in a moving daily time windows or time windows with fixed number of seismic tremors. Time changes of these parameters are then compared with mean value estimated in the analyzed area. This is the basis to indicate the zones of high seismic and rock burst hazard in specific moment in time during mining process. Additionally, the zones of high seismic and rock burst hazard are determined by utilization of passive seismic tomography method. All the calculated seismic parameters in moving time windows are used to quantify seismic and rock burst hazard by four level scales. In practice, assessment of seismic and rock burst hazard is used to make daily decision about using rock burst prevention activities and correction of further exploitation of monitored coal panel.
Development of antibiotic-loaded silk fibroin/hyaluronic acid polyelectrolyte film coated CoCrMo alloy
Authors: Arpaçay Pınar , Türkan Uğur 
Published: November 2015

AbstractBacteria related infections are still a major problem for the implant materials. Such infections have occurred in nearly 3% of hip and knee replacements resulting in failure of device. There are two main approaches for inhibiting the bacterial adhesion to the surface. These involve bactericidal substances and anti-adhesive coatings. In this study, the efficiency of antibiotic-loaded silk fibroin/hyaluronic acid polyelectrolyte film coated CoCrMo alloy, prepared by means of complex coacervate and layer by layer techniques, was investigated. A medical grade CoCrMo was coated with variable number of silk fibroin/hyaluronic acid up to 14 layers at room temperature. The morphological evolution during and after formation of the crystal structure on the coating layer, the resulting surface roughness, and the corresponding alterations in the coating layer thicknesses were thoroughly studied using various analytical techniques, including attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM) and atomic force microscopy (AFM). As a result, only 10 layers of silk fibroin/hyaluronic acid complex coacervate films were found to convey the general characteristics of the mixture of silk I and II, while layer by layer coated samples exhibited the mixture of silk I and II. Moreover, regardless of the preparation method applied, the surface roughness and the coating layer thicknesses were determined to increase with the increasing number of layers. The antibacterial test results suggested that the samples loaded with antibiotic successfully induced a bactericidal resistance against
Seasonal and spatial variation in biochemical composition of Saccharina latissima during a potential harvesting season for Western Sweden
Authors: Vilg Jenny Veide , Nylund Göran M. , Werner Tony , Qvirist Linnea , Mayers Joshua J. , Pavia Henrik , Undeland Ingrid , Albers Eva 
Published: November 2015

Abstract
This study monitored the biomass composition of Saccharina latissima during a potential harvesting season on the West coast of Sweden, in order to find suitable harvest times for biorefinery purposes. Specimens of S. latissima were sampled at three locations in June, August and October and the biomass was analysed for its macromolecular composition, as well as for the content of several specific compounds, e.g. sugars and fatty acids. PERMANOVA analyses showed that there was a significant difference in the biomass composition among time points. The total carbohydrate concentration was lowest in June and peaked at 360 mg g-1 dry weight in August, while the mannitol content was highest, 90 mg g-1, in June and decreased throughout the sampling period. Total protein and fatty acid concentrations were found to be approximately 80 and 3 mg g-1, respectively, with relatively little variation over time. Overall, there was little spatial variation in the macromolecular composition, although the concentration of some specific monosaccharides and fatty acids, as well as the total phenolic content, differed among localities. We discuss the implications of the observed variation in biomass composition of S. latissima for future biorefinery purposes.
In silico screening of alleged miRNAs associated with cell competition: an emerging cellular event in cancer
Authors: Patel Manish , Antala Bhavesh , Shrivastava Neeta 
Published: November 2015

AbstractCell competition is identified as a crucial phenomenon for cancer and organ development. There is a possibility that microRNAs (miRNAs) may play an important role in the regulation of expression of genes involved in cell competition. In silico screening of miRNAs is an effort to abridge, economize and expedite the experimental approaches to identification of potential miRNAs involved in cell competition, as no study has reported involvement of miRNAs in cell competition to date. In this study, we used multiple screening steps as follows: (i) selection of cell competition related genes of Drosophila through a literature survey; (ii) homology study of selected cell competition related genes; (iii) identification of miRNAs that target conserved cell competitionrelated genes through prediction tools; (iv) sequence conservation analysis of identified miRNAs with human genome; (v) identification of conserved cell competition miRNAs using their expression profiles and exploration of roles of their homologous human miRNAs. This study led to the identification of nine potential cell competition miRNAs in the Drosophila genome. Importantly, eighteen human homologs of these nine potential Drosophila miRNAs are well reported for their involvement in different types of cancers. This confirms their probable involvement in cell competition as well, because cell competition is well justified for its involvement in cancer initiation and maintenance.
Homology arms of targeting vectors for gene insertions and CRISPR/Cas9 technology: size does not matter; quality control of targeted clones does
Authors: Petrezselyova Silvia , Kinsky Slavomir , Truban Dominika , Sedlacek Radislav , Burtscher Ingo , Lickert Heiko 
Published: November 2015

AbstractClustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) technology has brought rapid progress in mammalian genome editing (adding, disrupting or changing the sequence of specific sites) by increasing the frequency of targeted events. However, gene knock-in of DNA cassettes by homologous recombination still remains difficult due to the construction of targeting vectors possessing large homology arms (from 2 up to 5 kb). Here, we demonstrate that in mouse embryonic stem cells the combination of CRISPR/Cas9 technology and targeting vectors with short homology arms (~ 0.3 kb) provides sufficient specificity for insertion of fluorescent reporter cassettes into endogenous genes with similar efficiency as those with large conventional vectors. Importantly, we emphasize the necessity of thorough quality control of recombinant clones by combination of the PCR method, Southern hybridization assay and sequencing to exclude undesired mutations. In conclusion, our approach facilitates programmed integration of exogenous DNA sequences at a target locus and thus could serve as a basis for more sophisticated genome engineering approaches, such as generation of reporters and conditional knock-out alleles.
Bryophytes of the Čantoria National Nature Reserve (Moravskoslezské Beskydy Mts., Czech Republic)
Authors: Plášek Vítězslav 
Published: November 2015

Abstrac
t Bryological investigation in National Nature Reserve Čantoria was done in 2013. The total of 99 bryophytes (29 liverworts and 70 moss species) recorded and/or collected are listed in this contribution. The most important records included 2 liverworts (Liochlaena lanceolata and Nowellia curvifolia) and 4 mosses (Brachydontium trichodes, Campylostelium saxicola, Fissidens pusillus and Oxystegus tenuirostris).
Determination of the Prosumer’s Optimal Bids
Authors: Ferruzzi Gabriella , Rossi Federico , Russo Angela 
Published: November 2015

Abstract
This paper considers a microgrid connected with a medium-voltage (MV) distribution network. It is assumed that the microgrid, which is managed by a prosumer, operates in a competitive environment and participates in the day-ahead market. Then, as the first step of the short-term management problem, the prosumer must determine the bids to be submitted to the market. The offer strategy is based on the application of an optimization model, which is solved for different hourly price profiles of energy exchanged with the main grid. The proposed procedure is applied to a microgrid and four different its configurations were analyzed. The configurations consider the presence of thermoelectric units that only produce electricity, a boiler or/and cogeneration power plants for the thermal loads, and an electric storage system. The numerical results confirmed the numerous theoretical considerations that have been made.
Critical soil bulk density for soybean growth in Oxisols
Authors: Keisuke Sato Michel , Veras de Lima Herdjania , Oliveira Pedro Daniel de , Rodrigues Sueli 
Published: November 2015

Abstract
The aim of this study was to evaluate the critical soil bulk density from the soil penetration resistance measurements for soybean root growth in Brazilian Amazon Oxisols. The experiment was carried out in a greenhouse using disturbed soil samples collected from the northwest of Para characterized by different texture. The treatments consisted of a range of soil bulk densities for each soil textural class. Three pots were used for soybean growth of and two for the soil penetration resistance curve. From the fitted model, the critical soil bulk density was determined considering the penetration resistance values of 2 and 3 MPa. After sixty days, plants were cut and root length, dry mass of root, and dry mass of shoots were determined. At higher bulk densities, the increase in soil water content decreased the penetration resistance, allowing unrestricted growth of soybean roots. Regardless of soil texture, the penetration resistance of 2 and 3 MPa had a slight effect on root growth in soil moisture at field capacity and a reduction of 50% in the soybean root growth was achieved at critical soil bulk density of 1.82, 1.75, 1.51, and 1.45 Mg m-3 for the sandy loam, sandy clay loam, clayey, and very clayey soil.
Effect of tillage system on soil temperature in a rainfed Mediterranean Vertisol
Authors: Muñoz-Romero Veronica , Lopez-Bellido Luis , Lopez-Bellido Rafael J. 
Published: November 2015

Abstract
Soil temperature is a factor that influences the rates of physical, chemical, and biological reactions in soils and has a strong influence on plant growth. A field study was conducted during 2006-2007 and 2009-2010 on a typical rainfed Mediterranean Vertisol to determine the effects of the tillage system and the crop on soil temperature. The experimental treatments were the tillage system (no-tillage and conventional tillage) and the crop (wheat and faba bean). Soil temperature was measured at a 20 cm depth at 1 h intervals from December 1st to November 30th of 2006-2007 and 2009-2010. There was a highly significant relationship between air temperature (both maximum and minimum) and soil temperature for the two tillage systems. Soil temperature was similar in the growing season for both crops but was higher in the conventional tillage than in the no-tillage system, with differences between 0.7 and 2.6°C depending on the month of the year. A higher soil temperature with conventional tillage can be beneficial in the cold sowing period (November-December), improving crop establishment. In contrast, in critical periods with water deficits (spring) during which grain formation occurs, the lower temperature corresponding to the no-tillage system would be more favourable.
Application of a tangent curve mathematical model for analysis of the mechanical behaviour of sunflower bulk seeds
Authors: Sigalingging Riswanti , Herák David , Kabutey Abraham , Dajbych Oldřich , Hrabě Petr , Mizera Čestmír 
Published: November 2015

AbstractThis paper evaluate the use of a tangent curve mathematical model for representation of the mechanical behaviour of sunflower bulk seeds. Compression machine (Tempos Model 50, Czech Republic) and pressing vessel diameter 60 mm were used for the loading experiment. Varying forces between 50 and 130 kN and speeds ranging from 10, 50, and 100 mm min-1were applied respectively on the bulk seeds with moisture content 12.37±0.38% w.b. The relationship between force and deformation curves of bulk seeds of pressing height 80 mm was described. The oil point strain was also determined from the different deformation values namely 30, 35, 40, and 45 mm at speed 10 mm min-1. Based on the results obtained, model coefficients were determined for fitting the experimental load and deformation curves. The validity of these coefficients were dependent on the bulk seeds of pressing height, vessel diameter, maximum force 110 kN, and speed 10 mm min-1, where optimal oil yield was observed. The oil point was detected at 45 mm deformation giving the strain value of 0.56 with the corresponding force 16.65±3.51 kN and energy 1.06±0.18 MJ m-3. At the force of 130 kN, a serration effect on the curves was indicated; hence, the compression process was ceased.
Anti-inflammatory effects of the cannabidiol derivative dimethylheptyl-cannabidiol – studies in BV-2 microglia and encephalitogenic T cells
Authors: Juknat Ana , Kozela Ewa , Kaushansky Nathali , Mechoulam Raphael , Vogel Zvi 
Published: November 2015

Abstract

Background: Dimethylheptyl-cannabidiol (DMH-CBD), a non-psychoactive, synthetic derivative of the phytocannabinoid cannabidiol (CBD), has been reported to be anti-inflammatory in RAW macrophages. Here, we evaluated the effects of DMH-CBD at the transcriptional level in BV-2 microglial cells as well as on the proliferation of encephalitogenic T cells.

Methods: BV-2 cells were pretreated with DMH-CBD, followed by stimulation with the endotoxin lipopolysaccharide (LPS). The expression levels of selected genes involved in stress regulation and inflammation were determined by quantitative real-time PCR. In addition, MOG35–55-reactive T cells (TMOG) were cultured with antigen-presenting cells in the presence of DMH-CBD and MOG35–55 peptide, and cell proliferation was determined by measuring [3H]thymidine incorporation.

Results: DMH-CBD treatment downregulated in a dose-dependent manner the mRNA expression of LPS-upregulated pro-inflammatory genes (Il1b, Il6, and Tnf) in BV-2 microglial cells. The expression of these genes was also downregulated by DMH-CBD in unstimulated cells. In parallel, DMH-CBD upregulated the expression of genes related to oxidative stress and glutathione homeostasis such as Trb3, Slc7a11/xCT, Hmox1, Atf4, Chop, and p8 in both stimulated and unstimulated microglial cells. In addition, DMH-CBD dose-dependently inhibited MOG35–55-induced TMOG proliferation.

Conclusions: The results show that DMH-CBD has similar anti-inflammatory properties to those of CBD. DMH-CBD downregulates the expression of inflammatory cytokines and protects the microglial cells by inducing an adaptive cellular response against inflammatory stimuli and oxidative injury. In addition, DMH-CBD decreases the proliferation of pathogenic activated TMOG cells.
Oxytocin utilization for labor induction in obese and lean women
Authors: Hill Meg , Reed Kathryn L. , Cohen Wayne R. 
Published: November 2015

Abstract
To determine if oxytocin dosage used for labor induction differed in obese and lean women, we analyzed records of patients who underwent term labor induction and delivered vaginally. Each of a cohort of 27 patients with a body mass index (BMI) >40 kg/m2 was matched with a patient with a BMI <28 kg/m2 for gestational age, for birth weight, and for cervical dilatation and fetal station at admission. The oxytocin dose administered during first stage labor was calculated for each patient. In addition to the matched characteristics, there was no difference between groups in parity, frequency of diabetes, epidural anesthesia use, or pharmacologic cervical ripening. Oxytocin utilization was significantly greater in obese women than in lean women. The maximum administration rate was 17.7±4.7 and 13.1±5.0 mU/min, respectively (P=0.001). Oxytocin administered per minute during the first stage of labor was greater in the obese group (11.6±4.8 vs. 8.6±4.1 mU/min; P=0.020). Neither active phase duration nor the maximum rate of dilatation differed significantly between the groups. That obese parturients required more oxytocin than lean women during the first stage of successful labor induction could not be explained by group differences in parity, birth weight, dysfunctional labor, pre-induction dilatation and station, or epidural use.