bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of reef fish with oil and gas platforms in the Northern Gulf of Mexico
Authors: Fujiwara Masami , Beyea R. Taylor , Putman Nathan F. 
Published: July 2024

AbstractOffshore oil and gas platforms support abundant reef fish and are popular fishing sites for recreational anglers. However, the rapid decommissioning and removal of active platforms have decreased such fishing opportunities in the Gulf of Mexico, raising concerns about fisheries impacts. Conversely, planned offshore energy structures like wind turbines may offer similar habitats and fishing sites. To inform spatial planning for marine energy infrastructure in the context of recreational fisheries, we created models of fish communities associated with oil and gas platforms using existing abundance data. We employed Random Forest analysis to predict the presence‐absence and abundance of Red Snapper (Lutjanus campechanus) and Greater Amberjack (Seriola dumerili) at platforms using 47 environmental and platform variables. Nonmetric multidimensional scaling on Bray−Curtis dissimilarities explored fish species composition among 37 species. Results showed variability in Red Snapper and Greater Amberjack incidence/abundance from shore to shelf‐edge, not attributed to surrounding habitat or climatological oceanographic variables. Incidence models were more robust than abundance models. Fish species composition was significantly influenced by location gradient, with less impact from other habitat features. Our findings guide selecting areas for artificial structures to enhance angler opportunities and maintain fish diversity, but identifying the drivers of finer scale abundance variation will require further sampling.
Medical costs and incremental medical costs of asthma among workers in the United States
Authors: Bhattacharya Anasua , Syamlal Girija , Dodd Katelynn E. 
Published: July 2024

AbstractBackgroundAsthma, a chronic respiratory disease, is associated with high economic burden. This study estimates per‐worker medical and incremental medical costs associated with treated asthma by socioeconomic and demographic characteristics, industries, medical events, and sources of payments for workers aged ≥18 years.MethodsWe analyzed Medical Expenditure Panel Survey data from 2018 to 2020 to assess medical costs for treated asthma among workers using the International Classification of Diseases, Tenth Revision, Clinical Modification code for asthma (J45). We used two‐part regression models to estimate medical and incremental medical costs controlling for covariates. All results are adjusted for inflation and presented in 2022 US dollar values.ResultsAn estimated annual average of 8.2 million workers out of 176 million had at least one medical event associated with treated asthma. The annualized estimated per‐worker incremental medical costs for those with treated asthma was $457 and was highest among: those in the age group of 35–44 years ($534), in the western region ($768), of Hispanic ethnicity ($693), employed in the utility and transportation industries ($898), males ($650), and for inpatient admissions ($754). The total annualized medical costs of treated asthma was $21 billion and total of incremental medical costs was $3.8 billion.ConclusionFindings of higher incremental medical costs for treated asthma among workers in certain socioeconomic, demographic, and industry groups highlight the economic benefit of prevention and early intervention to reduce morbidity of asthma in working adults. Our results suggest that the per‐person incremental medical costs of treated asthma among workers are lower than that for all US adults.
Effect of gamification with a support partner to increase physical activity in older adults at risk for Alzheimer's disease: The STEP 4Life randomized clinical trial
Authors: Greysen S. Ryan , Oon Ai Leen , Harkins Kristin , Rareshide Charles , Mondal Angira , Patel Mitesh S. , Grill Joshua D. , Karlawish Jason 
Published: July 2024

AbstractINTRODUCTIONPhysical activity is associated with reduced risk of cognitive and functional decline but scalable, sustainable interventions for populations at risk for Alzheimer's disease (AD) and AD and related dementias (ADRD) are lacking.METHODSA 12‐week randomized‐controlled trial was conducted with a 3‐week follow‐up using a national AD prevention registry (GeneMatch). The control group (n = 50) set step goals and received daily feedback. The intervention group (n = 44) also received a behaviorally designed game based on achieving step goals and reinforced by a support partner.RESULTSIntervention participants (94 participants, mean age 70, 78% female) had greater change in mean daily step count than control of 1699 steps/day (95% confidence interval [CI], 1149–2249), P < 0.0001, which was sustained in the follow‐up period at 1219 steps/day (95% CI, 455–1983), P = 0.0018. Carriers of the apolipoprotein E ε4 gene (high risk) did not perform differently than non‐carriers; however, high self‐reported risk perception was associated with higher activity.DISCUSSIONA gamified intervention was effective in promoting and sustaining higher physical activity in older adults at genetic risk for AD/ADRD.Highlights
A simple game played with a support partner increased walking in older adults at risk for Alzheimer's disease (AD).
The game also increased minutes of moderate‐to‐vigorous physical activity per day.
Perception of lifelong AD risk was associated with increased activity but genetic risk (apolipoprotein E ε4+) was not.
TRIAL REGISTRATIONClinicalTrials.gov: NCT05069155
Regioselective Oxidative Phenol Coupling by a Mushroom Unspecific Peroxygenase
Authors: Platz Lukas , Löhr Nikolai A. , Girkens Max P. , Eisen Frederic , Braun Konstantin , Fessner Nico , Bär Christian , Hüttel Wolfgang , Hoffmeister Dirk , Müller Michael 
Published: July 2024

AbstractBioactive dimeric (pre‐)anthraquinones are ubiquitous in nature and are found in bacteria, fungi, insects, and plants. Their biosynthesis via oxidative phenol coupling (OPC) is catalyzed by cytochrome P450 enzymes, peroxidases, or laccases. While the biocatalysis of OPC in molds (Ascomycota) is well‐known, the respective enzymes in mushroom‐forming fungi (Basidiomycota) are unknown. Here, we report on the biosynthesis of the atropisomers phlegmacin A1 and B1 of the mushroom Cortinarius odorifer. The biosynthesis of these unsymmetrically 7,10’‐homo‐coupled dihydroanthracenones was heterologously reconstituted in the mold Aspergillus niger. Methylation of the parental monomer atrochrysone to its 6‐O‐methyl ether torosachrysone by the O‐methyltransferase CoOMT1 precedes the regioselective homocoupling to phlegmacin, catalyzed by the enzyme CoUPO1 annotated as an “unspecific peroxygenase” (UPO). Our results reveal an unprecedented UPO reaction, thereby expanding the biocatalytic portfolio of oxidative phenol coupling beyond the commonly reported enzymes. The results show that Basidiomycota use peroxygenases to selectively couple aryls independently of and convergently to any other group of organisms, emphasizing the central role of OPC in natural processes.
Association of Autoantibody Concentrations and Trajectories With Lupus Nephritis Histologic Features and Treatment Response
Authors: Fava Andrea , Wagner Catriona A. , Guthridge Carla J. , Kheir Joseph , Macwana Susan , DeJager Wade , Gross Tim , Izmirly Peter , Belmont H. Michael , Diamond Betty , Davidson Anne , Utz Paul J. , Weisman Michael H , Magder Laurence S. ,   the Accelerating Medicines Partnership Rheumatoid Arthritis and Systemic Lupus Erythematosus (AMP RA/SLE) Network, Guthridge Joel M. , Petri Michelle , Buyon Jill , James Judith A. 
Published: July 2024

ObjectiveAutoantibodies are a hallmark of lupus nephritis (LN), but their association with LN classes and treatment response are not adequately known. In this study, we quantified circulating autoantibodies in the Accelerating Medicines Partnership LN longitudinal cohort to identify serological biomarkers of LN histologic classification and treatment response and how these biomarkers change over time based on treatment response.MethodsPeripheral blood samples were collected from 279 patients with systemic lupus erythematosus undergoing diagnostic kidney biopsy based on proteinuria. Of these, 268 were diagnosed with LN. Thirteen autoantibody specificities were measured by bead‐based assays (Bio‐Rad Bioplex 2200) and anti‐C1q by enzyme‐linked immunosorbent assay at the time of biopsy (baseline) and at 3, 6, and 12 months after biopsy. Clinical response was determined at 12 months.ResultsProliferative LN (International Society of Nephrology/Renal Pathology Society class III/IV±V, n = 160) was associated with higher concentrations of anti‐C1q, anti‐chromatin, anti–double‐stranded DNA (dsDNA), and anti–ribosomal P autoantibodies compared to nonproliferative LN (classes I/II/V/VI, n = 108). Anti‐C1q and‐dsDNA were independently associated with proliferative LN. In proliferative LN, higher baseline anti‐C1q levels predicted complete response (area under the curve [AUC] 0.72; P = 0.002) better than baseline proteinuria (AUC 0.59; P = 0.21). Furthermore, all autoantibody levels except for anti‐La/SSB decreased over 12 months in patients with proliferative, but not membranous, LN with a complete response.ConclusionBaseline levels of anti‐C1q and anti‐dsDNA may serve as noninvasive biomarkers of proliferative LN, and anti‐C1q may predict complete response at the time of kidney biopsy. In addition, tracking autoantibodies over time may provide further insights into treatment response and pathogenic mechanisms in patients with proliferative LN.image
Learning in context: Undergraduate students' knowledge and the content retention of anatomy between discipline‐specific and integrated course approaches
Authors: Adams Jessica A. , Dewsbury Bryan M. , Tanzer Joshua R. 
Published: July 2024

AbstractUndergraduate introductory human anatomy and human physiology courses are either taught as discipline‐specific or integrated anatomy and physiology (A&P) sequences. An institution underwent a curricular revision to change the course approach from discipline‐specific Human Anatomy and Human Physiology to an integrated A&P I and II sequence, allowing the unique opportunity to explore the potential role of contextual learning in academic achievement and content retention. Mediation and moderation analysis was used to evaluate lecture examinations, laboratory practical examinations, and anatomical content retention between the different course approaches. Undergraduate students in the integrated A&P I course approach performed significantly better on lecture assessments and had a higher anatomy content retention rate at the end of the year than students enrolled in the standalone Human Anatomy course. The lecture examination averages between Human Physiology and A&P II (the second course in the sequence), as well as the anatomy laboratory practical examinations, were not significantly different between discipline‐specific and integrated course approaches. The results suggest contextual learning—providing physiological context to anatomical structures—increases the anatomical content retention and academic achievement overall.
Harnessing Solar Power for Oxidation of Organic Compounds by Re(I)Dipyrrinato Complexes
Authors: Kumar Shekhar , Agarwal Kritika , Sanyam  , Mondal Anirban , Gupta Iti 
Published: July 2024

AbstractMetal dipyrrinato complexes of 4d and 5d metals have distinctive features such as high absorption coefficients in the visible section and room temperature phosphorescence in the red region. This work demonstrates the light‐assisted oxidation of organic compounds employing rhenium(I)dipyrrinato complexes as catalysts. The heavy atom effect in rhenium(I)dipyrrinato complexes leads to the formation of long‐lived triplet excited states, and these complexes can generate singlet oxygen in excellent yields (up to 84 %). A method was developed for photocatalytic aerobic oxidation of sulfides and amines using only 0.05 mol % and 0.025 mol % of the rhenium(I)dipyrrinato complexes, respectively. The method is efficient, and within 2 h, a variety of substrates were oxidized to produce sulfoxides and imines in high yields (up to 97 %). Rhenium(I)dipyrrinato complexes work very well both in visible light and sunlight, making them promising candidates for photocatalytic applications.
Fractional order modeling and internal model control method for dielectric elastomer actuator
Authors: Xu Zhichao , Wu Jundong , Wang Yawu 
Published: July 2024

AbstractThe dielectric elastomer actuator (DEA) is widely used in the field of soft robots due to its large deformation, light weight, fast response, and high‐energy conversion efficiency. The high‐precision control of the DEA is the precondition for soft robots to perform complicated tasks. In early studies, researchers usually employed integer order modeling and control methods to build the dynamic model of the DEA and to achieve its tracking control. However, these methods are not good at handling the complicated memory property of the DEA. In addition, the number of required parameters in integer order models and control methods is enormous, which hinders their practical applications. To solve these problems, the fractional order modeling method and fractional order internal model control method of the DEA are proposed in this paper. Firstly, a fractional order transfer function (FOTF) model of the DEA is built to depict its complicated memory property. Then, to achieve the computer control, an integer order approximation model (IOAM) of the FOTF model is built by using the Oustaloup filter. Considering that the order of the IOAM is too high, a reduced integer order approximation model is established by using the square root balance truncation algorithm to facilitate the system controller design. Next, a fractional order internal model controller is designed. Finally, tracking control experiments are exerted to demonstrate the effectiveness of the proposed method. Since the root‐mean‐square errors of all experimental results are less than 2%, the proposed modeling method and control method are superior from the perspective of the practical application.
Advances in xenogeneic donor decellularized organs: A review on studies with sheep and porcine‐derived heart valves
Authors: Inal Muslum Suleyman , Avci Huseyin , Hassan Shabir , Darcan Cihan , Shin Su Ryon , Akpek Ali 
Published: July 2024

AbstractHeart valve replacement surgeries are performed on patients suffering from abnormal heart valve function. In these operations, the problematic tissue is replaced with mechanical valves or with bioprosthetics that are being developed. The thrombotic effect of mechanical valves, reflecting the need for lifelong use of anticoagulation drugs, and the short‐lived nature of biological valves make these two types of valves problematic. In addition, they cannot adapt to the somatic growth of young patients. Although decellularized scaffolds have shown some promise, a successful translation has so far evaded. Although decellularized porcine xenografts have been extensively studied in the literature, they have several disadvantages, such as a propensity for calcification in the implant model, a risk of porcine endogenous retrovirus (PERV) infection, and a high xenoantigen density. As seen in clinical data, it is clear that there are biocompatibility problems in almost all studies. However, since decellularized sheep heart valves have not been tried in the clinic, a large data pool could not be established. This review compares and contrasts decellularized porcine and sheep xenografts for heart valve tissue engineering. It reveals that decellularized sheep heart valves can be an alternative to pigs in terms of biocompatibility. In addition, it highlights the potential advantages of bioinks derived from the decellularized extracellular matrix in 3D bioprinting technology, emphasizing that they can be a new alternative for the application. We also outline the future prospects of using sheep xenografts for heart valve tissue engineering.
Emerging importance of stool preservation methods in OMICS studies with special focus on cancer biology
Authors: Mehra Parul , Kumar Anil 
Published: July 2024

AbstractThe intricate consortium of microorganisms in the human gut plays a crucial role in different physiological functions. The complex known‐unknown elements of the gut microbiome are perplexing and the absence of standardized procedures for collecting and preserving samples has hindered continuous research in comprehending it. The technological bias produced because of lack of standard protocols has affected the reproducibility of results. The complex nature of diseases like colorectal cancer, gastric cancer, hepatocellular carcinoma and breast cancer require a thorough understanding of its etiology for an efficient and timely diagnosis. The designated protocols for collection and preservation of stool specimens have great variance, hence generate inconsistencies in OMICS studies. Due to the complications associated to the nature of sample, it is important to preserve the sample to be studied later in a laboratory or to be used in the future research purpose. Stool preservation is gaining importance due to the increased use of treatment options like fecal microbiota transplantation to cure conditions like recurrent Clostridium difficile infections and for OMICS studies including metagenomics, metabolomics and culturomics. This review provides an insight into the importance of omics studies for the identification and development of novel biomarkers for quick and noninvasive diagnosis of various diseases.
Transcriptome‐wide profiling for melanocytes derived from newborn and adult human epidermis with enhanced proliferation
Authors: Orimoto Ai , Kashiwagi Sayo , Funakoshi Ayaka , Shimizu Takashi , Ishii Tsuyoshi , Kiyono Tohru , Fukuda Tomokazu 
Published: July 2024

AbstractThe senescence‐associated protein p16INK4A acts as a limiter element in cell‐cycle progression. The loss of p16INK4A function is causally related to cellular immortalization. The increase in p16INK4A levels with advancing age was demonstrated in melanocytes. However, the characteristic difference between young and senescent melanocytes affecting immortalization of melanocytes remains unclear. In this study, we generated 10 different cell lines in total from newborn (NB) and adult (AD) primary normal human epidermal melanocytes (NHEM) using four different methods, transduction of CDK4R24C and cyclin D1 (K4D), K4D with TERT (K4DT), SV40 T‐antigen (SV40T), and HPV16 E6 and E7 (E6/E7) and performed whole transcriptome sequencing analysis (RNA‐Seq) to elucidate the differences of genome‐wide expression profiles among cell lines. The analysis data revealed distinct differences in expression pattern between cell lines from NB and AD although no distinct biological differences were detected in analyses such as comparison of cell morphology, evaluation of cell proliferation, and cell cycle profiles. This study may provide useful in vitro models to benefit the understanding of skin‐related diseases.
Yeast Surface Display Enables One‐Step Production and Immobilization of Unspecific Peroxygenases
Authors: Teetz Niklas , Lang Selina , Liese Andreas , Holtmann Dirk 
Published: July 2024

AbstractUnspecific peroxygenases (UPOs) are regarded as a “dream catalyst” for selective oxyfunctionalization reactions like oxygenations. We present the display of the model UPO rAaeUPO (PaDa−I) on the cell surface of the heterologous production host Komagataella phaffii as a one‐step production and immobilization process. The coding sequence for PaDa−I was combined with genes coding for cell wall proteins from Saccharomyces cerevisiae and transformed into K. phaffii. The fusion proteins were compared among each other and with secreted, free PaDa−I. One system in particular, a C‐terminal fusion of PaDa−I and Sag1 yielded near identical activity per volume culture broth to the secreted PaDa−I with ~90 % of the activity being at the cell wall. The surface display simplifies downstream processing and includes immobilization on a cheap, retainable and replaceable matrix, that is the production host itself. The enzymes remained active in a repeated batch process for 10 batches and 200 h of catalysis.
Oxidative N−N Bond Formation Versus the Curtius Rearrangement
Authors: Hohenadel Melissa , Ebel Ben , Oppel Iris M. , Patureau Frederic W. 
Published: July 2024

AbstractThe oxidative formation of N−N bonds from primary amides has been recently reported and then retracted in the journal Nature Communications by Kathiravan, Nicholls, and co‐authors, utilizing a hypervalent iodane reagent. Unfortunately, the authors failed to recognize the Curtius reaction taking place under the described reaction conditions. Thus, the claimed N−N coupling products were not formed. Instead, the Curtius rearrangement urea coupling products were obtained. We demonstrate this herein by means of NMR and x‐ray analysis, as well as with the support of an alternative synthetic route.
Nitrogen removal and microbial community variation in a shallow constructed wetland with Fe–C porous filter material as substrate
Authors: Chu Shuyi , Cao Zhuangzhuang , Su Zhongping , Xiao Jibo , Li Jun 
Published: July 2024

AbstractThis study aims to examine the nitrogen removal characteristics and microbial community variation at low hydraulic retention time (HRT) in a shallow constructed wetland (SCW) using iron–carbon (Fe–C) porous filter material (PFM) as substrate. Effects of influent nitrogen forms and chemical oxygen demand (COD)/N ratio on nitrogen removal performance at HRT of 1 day were investigated. Results showed that total nitrogen (TN) removal declined with the decrease of influent NH4+‐N‐to‐NO3−‐N ratio. When the influent NH4+‐N/NO3−‐N ratio was 0.1, TN removal decreased by 15.4% compared with that at ratio of 2.0. The increase of influent COD/N ratio enhanced NO3−‐N reduction, and TN removal reached 74.5% at influent COD/N ratio 7.0. The microbial community was analyzed for the biofilm samples on Fe–C PFM at front (WF), middle (WM), and back (WB) of SCW. Proteobacteria, Bacteroidetes, Chloroflexi, and Firmicutes were dominant bacteria phyla. The relative abundance of genera involved in the nitrification and denitrification decreased with the influent flow. The iron autotrophic denitrifying and macromolecular organics degrading bacteria were abundant in the middle and back of SCW. Microbial nitrification and denitrification, plant uptake, and plant synergism contributed to 86.3%, 7.41%, and 19.9% of N removal, respectively. These results demonstrated that the SCW with Fe–C PFM as substrate was efficient in nitrogen removal at low HRT.