bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human papillomavirus (HPV) E6/E7 mRNA as a triage test after detection of HPV 16 and HPV 18 DNA
Authors: Perez Castro Sonia , Iñarrea Fernández Amparo , Lamas González María José , Sarán Diez María Teresa , Cid Lama Ana , Alvarez Martín María Jesús , Pato Mosquera Mónica , López‐Miragaya Isabel , Estévez Nuria , Torres Piñón Julio , Oña Navarro María 
Published: April 2013

AbstractHigh‐risk human papillomavirus (HPV) DNA detection provides high sensitivity but low specificity for moderate‐grade cervical intraepithelial neoplasia or worse histological identification. A prospective study evaluated mRNA testing efficacy for predicting this histological diagnosis in case of HPV 16 and/or 18 DNA detection. A total of 165 endocervical samples harboring HPV 16 and/or 18 DNA were tested with NucliSENS‐EasyQ® HPV E6/E7‐mRNA‐assay (Biomerieux, Marcy l´Etoile, France). Women with cytological alterations were referred to colposcopy (n = 111). Moderate‐grade cervical intraepithelial neoplasia or worse was diagnosed in 25.8% of women presenting atypical squamous cells of undetermined significance or low‐grade squamous intraepithelial lesions and in 89.8% of women with high‐grade squamous intraepithelial lesions. mRNA sensitivity was 81.3% and 84.1%, respectively. Specificity was 52.2%, and 80.0%, respectively. Negative predictive value (NPV) was 88.9% in undetermined or low‐grade squamous lesions. Positive predictive value (PPV) was 97.4% in high‐grade squamous lesions. mRNA reduced colposcopies by 44.3% in undetermined or low‐grade squamous lesions. Direct treatment of mRNA‐positive cases reduced 77.5% of colposcopies in high‐grade squamous lesions. Women without cytological alterations were followed for 18 months (n = 35), and moderate‐grade cervical intraepithelial neoplasia or worse was diagnosed in 34.3%; mRNA sensitivity and specificity were 83.3% and 86.9%, respectively. PPV and NPV were 76.9% and 90.9%, respectively for predicting moderate‐grade cervical intraepithelial neoplasia or worse in 18 months. mRNA reduced the number of visits for follow‐up in 62.2%. In conclusion, NucliSENS‐EasyQ® HPV E6/E7‐mRNA‐assay (Biomerieux) can serve as a triage test in case of HPV 16 and/or 18 DNA detection. J. Med. Virol. 85: 1063–1068, 2013. © 2013 Wiley Periodicals, Inc.
Antiviral therapy of symptomatic HCV‐associated mixed cryoglobulinemia: Meta‐analysis of clinical studies
Authors: Fabrizi Fabrizio , Dixit Vivek , Messa Piergiorgio 
Published: April 2013

AbstractHepatitis C virus (HCV) infection may be associated with extra‐hepatic illness including mixed cryoglobulinemia. Evidence on HCV‐related mixed cryoglobulinemia in the non‐transplantation setting exists even if its appropriate management remains unclear. The cornerstone of treatment for symptomatic HCV‐associated mixed cryoglobulinemia is antiviral therapy but little is known about its activity. A systematic review of the literature with a meta‐analysis of clinical studies was performed in order to assess efficacy and safety of combination antiviral therapy for symptomatic HCV‐associated mixed cryoglobulinemia in non‐immunosuppressed individuals. The random effects model of DerSimonian and Laird was used, with heterogeneity and sensitivity analyses. The primary outcome was sustained virological response (as a measure of efficacy), and the secondary outcome was the rate of patients stopping (or dose reducing) antiviral agents (as a measure of tolerability). Ten clinical studies (300 unique patients) were identified; the rate of baseline kidney involvement ranged between 4% and 39%. The summary estimate of frequency of sustained viral response was 0.42 with a 95% confidence interval (CI) of 0.31; 0.54 (random effects model). Significant heterogeneity occurred (P = 0.00001; I2 = 77.6%). Stratified analysis showed higher efficacy in those studies using combination therapy with pegylated—than conventional IFN; the summary estimate of sustained viral response being 0.52 (95% CI, 0.40; 0.63) and 0.32 (95% CI, 0.15; 0.49), respectively. There was good association between viral and clinical response, weighted K 0.634 (95% CI, 0.455; 0.814), by a meta‐analysis at individual level on a subset of reports (n = 3; 74 unique patients). The summary estimate of frequency of patients stopping (or dose reducing) antiviral agents was 0.15 (95% CI, 0.08; 0.21); no heterogeneity occurred (P = 0.05; I2 = 51%). In summary, combination antiviral therapy (pegylated IFN plus ribavirin) gives satisfactory response in more than the half of patients with symptomatic mixed cryoglobulinemia associated with HCV. HCV‐related mixed cryoglobulinemia is uncommon in developed countries and this clearly hampers randomized controlled clinical trials aimed to evaluate efficacy and safety of antiviral therapy in non‐immunosuppressed individuals. J. Med. Virol. 85: 1019–1027, 2013. © 2013 Wiley Periodicals, Inc.
E6 and E7 variants of human papillomavirus‐16 and ‐52 in Japan, the Philippines, and Vietnam
Authors: Ishizaki Azumi , Matsushita Kaori , Hoang Huyen Thi Thanh , Agdamag Dorothy M. , Nguyen Cuong Hung , Tran Vuong Thi , Sasagawa Toshiyuki , Saikawa Kunikazu , Lihana Raphael , Pham Hung Viet , Bi Xiuqiong , Ta Van Thanh , Van Pham Thuc , Ichimura Hiroshi 
Published: April 2013

AbstractHuman papillomavirus (HPV) has several intragenotypic variants with different geographical and ethnic distributions. This study aimed to elucidate the distribution patterns of E6 and E7 (E6/E7) intragenotypic variants of HPV type 16 (HPV‐16), which is most common worldwide, and HPV‐52, which is common in Asian countries such as Japan, the Philippines, and Vietnam. In previous studies, genomic DNA samples extracted from cervical swabs were collected from female sex workers in these three countries and found to be positive for HPV‐16 or HPV‐52. Samples were amplified further for their E6/E7 genes using type‐specific primers and analyzed genetically. Seventy‐nine HPV‐16 E6/E7 genes were analyzed successfully and grouped into three lineages: European (Prototype), European (Asian), and African‐2. The prevalences of HPV‐16 European (Prototype)/European (Asian) lineages were 19.4%/80.6% (n = 31) in Japan, 75.0%/20.8% (n = 24) in the Philippines, and 0%/95.8% (n = 24) in Vietnam. The 109 HPV‐52 E6/E7 genes analyzed successfully were grouped into four lineages, A–D; the prevalences of lineages A/B/C/D were, respectively, 5.1%/92.3%/0%/2.6% in Japan (n = 39), 34.4%/62.5%/0%/3.1% in the Philippines (n = 32), and 15.8%/73.7%/7.9%/2.6% in Vietnam (n = 38). The distribution patterns of HPV‐16 and HPV‐52 lineages in these countries differed significantly (P < 0.000001 and P = 0.0048, respectively). There was no significant relationship between abnormal cervical cytology and either HPV‐16 E6/E7 lineages or specific amino acid mutations, such as E6 D25E, E6 L83V, and E7 N29S. Analysis of HPV‐16 and HPV‐52 E6/E7 genes can be a useful molecular‐epidemiological tool to distinguish geographical diffusion routes of these HPV types in Asia. J. Med. Virol. 85: 1069–1076, 2013. © 2013 Wiley Periodicals, Inc.
Snapshot on drug‐resistance rate and profiles in patients with chronic hepatitis B receiving nucleos(t)ide analogues in clinical practice
Authors: Salpini R. , Alteri C. , Cento V. , Pollicita M. , Micheli V. , Gubertini G. , De Sanctis G.M. , Visca M. , Romano S. , Sarrecchia C. , Andreoni M. , Angelico M. , Parruti G. , Svicher V. , Perno C.F. 
Published: April 2013

AbstractWhile the selection of complex HBV drug‐resistance patterns on therapeutic failure can compromise the efficacy of anti‐HBV therapies, recent data show that patients failing treatment without drug‐resistance have a rate of virological success close to drug‐naive patients. The goal of this study is defining, in clinical practice, the burden of drug‐resistance mutations in a cohort of patients treated with anti‐HBV drugs. Prevalence and patterns of drug‐resistance were analyzed by RT‐sequencing in 204 patients infected chronically: 148 experiencing virological rebound (defined as an increase in serum HBV‐DNA > 20 IU/ml after achieving virological success [HBV‐DNA < 20 IU/ml]), and 56 null/partial responders (always detectable serum HBV‐DNA [>20 IU/ml] within 48 weeks of therapy). The highest rate of drug‐resistance was observed in patients experiencing virological rebound (prevalence, 79.1%). Conversely, almost half (46.4%) null/partial responders have no evidence of drug‐resistance. The rate of drug‐resistance was higher in patients treated with lamivudine (76.8% [109/142]) and telbivudine (83.3% [5/6]), followed by adefovir (62.5% [15/24]), and entecavir (52.2% [12/23]). Complex mutational patterns characterized by the co‐presence of rtM204V/I‐rtA181T/V (impairing the efficacy of all anti‐HBV drugs) were detected in four patients (2.7%) with virological rebound. Drug‐resistance is the main cause of failure to therapy in patients experiencing virological rebound, supporting the need of rapid switch to anti‐HBV drugs with higher genetic barrier and potency (entecavir/tenofovir). Conversely, nearly half of null/partial responders shows no evidence of drug‐resistance mutations, maintaining high chance of achieving therapeutic success with the same class of drug. In this setting, genotypic resistance may help in selecting patients still carrying wild‐type viruses, that may take major benefits from antiviral treatment. J. Med. Virol. 85: 996–1004, 2013. © 2013 Wiley Periodicals, Inc.
How does spouse career support relate to employee turnover? Work interfering with family and job satisfaction as mediators
Authors: Huffman Ann H. , Casper Wendy J. , Payne Stephanie C. 
Published: April 2013

SummaryEmployee turnover is a major concern because of its cost to organizations. Although theory supports the influence of nonwork factors on turnover, our understanding of the degree to which nonwork factors relate to actual turnover behavior is not well developed. Using a sample of 5505 U.S. Army officers, we assessed the extent to which spouse career support related to reduced turnover four years later through work interfering with family (WIF) and job satisfaction as mechanisms. Results revealed that spouse career support decreased the odds of turnover, and WIF and job satisfaction sequentially mediated this relationship, with lower WIF and higher job satisfaction reducing the odds of turnover. Practical implications of using family support systems as retention interventions are discussed. Copyright © 2013 John Wiley & Sons, Ltd.
Visual comfort in 2D/3D modes of glasses‐type stereoscopic displays
Authors: Hung‐Shing Chen , Chung‐Cheng Huang , Ronnier Luo 
Published: April 2013

AbstractIn this study, we compared visual comfort in 2D/3D modes of the pattern retarder (PR) and shutter glasses (SG) stereoscopic displays by changing viewing factors and image contents. The viewing factors include ambient illuminance/monitor luminance/background luminance and image contents mainly are determined with different disparity limits. The degrees of 2D/3D visual comfort were investigated by using various combinations of ambient illuminance, monitor luminance, background luminance, and disparity limit. A series of psychological experiments were also performed to compare 2D and 3D viewing experiences for the passive PR and active SG stereoscopic displays and to discover more comfortable conditions under various variable combinations. The experiment results show that the various variable combinations affecting visual comfort in the passive PR and active SG stereoscopic displays were significantly different. Finally, we suggest more comfortable conditions of viewing 2D and 3D images for the PR and SG stereoscopic displays.
On the images of Sobolev spaces under the heat kernel transform on the Heisenberg group
Authors: Radha R. , Thangavelu S. , Naidu D. Venku 
Published: April 2013

AbstractThe aim of this paper is to obtain certain characterizations for the image of a Sobolev space on the Heisenberg group under the heat kernel transform. We give three types of characterizations for the image of a Sobolev space of positive order \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$H^m(\mathbb {H}^n), m\in \mathbb {N}^n,$\end{document} under the heat kernel transform on \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$\mathbb {H}^n,$\end{document} using direct sum and direct integral of Bergmann spaces and certain unitary representations of \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$\mathbb {H}^n$\end{document} which can be realized on the Hilbert space of Hilbert‐Schmidt operators on \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$L^2(\mathbb {R}^n).$\end{document} We also show that the image of Sobolev space of negative order \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$H^{-s}(\mathbb {H}^n), s(>0) \in \mathbb {R}$\end{document} is a direct sum of two weighted Bergman spaces. Finally, we try to obtain some pointwise estimates for the functions in the image of Schwartz class on \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$\mathbb {H}^n$\end{document} under the heat kernel transform.
Interaction of Plasma Polypyrrole Particles with Ionic Solutions
Authors: Gómez Lidia Ma. , Olayo Ma. Guadalupe , Cruz Guillermo J. , González‐Torres Maribel , López Osvaldo G. , De Jesús Carlos 
Published: April 2013

AbstractThis work presents a study about the hydrophilicity and conductivity of plasma polypyrroles (PPy) in contact with solutions of salt concentration similar to human tissues. PPy is a conjugated biocompatible polymer in which the electronic distribution produces partial relocation in contact with different compounds. PPy and PPy doped with iodine (PPy/I) particles of different sizes are being studied as implants in the central nervous system to restore the lost neuronal communication after severe injuries. Particle size, hydrophilicity and conductivity of these polymers are essential in this task. In this work, plasma PPy and PPy/I were synthesized as thin films using low‐pressure, rf, resistive, electrical glow discharges and grounded up to obtain microparticles in the range of neuronal nuclei. The electrical conductivity of particles was studied with the addition of human‐like ionic solutions, particularly with sodium and potassium ions which are part in the ionic transference of electric charges in neuronal membranes.
Synthesis of TiO‐PE Superficial Composites by Plasmas of Titanium Tetraisopropoxide
Authors: Olayo Ma. Guadalupe , Arreola Mirna L. , Cruz Guillermo J. , García‐Rosales Genoveva , González‐Castillo Susana , Gómez Lidia Ma. , González‐Torres Maribel 
Published: April 2013

AbstractThis work presents a study about the synthesis by plasma of organometallic compounds of titanium oxides (TiO) obtained from titanium tetraisopropoxide (TTIP) and water on polyethylene (PE) to form TiO‐PE superficial composites. The objective is the synthesis of TiO nano and meso particles attached on PE supports. The synthesis was carried out by plasma in a vacuum tubular glass reactor at 13.56 MHz, 10−1 mbar and 100–150 W. The accelerated particles in the plasma have the function of separating the organic and inorganic segments of TTIP bonding the TiO particles with PE. The resulting TiO‐PE superficial compounds have agglomerates of spherical and oval organometallic particles with diameter between 50 and 3000 nm as a function of the time of synthesis. The agglomerates contain Ti and C oxides with a tendency to change from film to particles as a function of the energy applied in the synthesis. The contact angles of the superficial composites varied from 40° to 76°.
Analysis of PPAR‐α/γ Activity by Combining 2‐D QSAR and Molecular Simulation
Authors: Vallianatou Theodosia , Lambrinidis George , Giaginis Costas , Mikros Emmanuel , Tsantili‐Kakoulidou Anna 
Published: April 2013

AbstractIn the present study 2D‐QSAR analysis was combined with information on crystallographic data and molecular modeling, in order to investigate dual PPAR‐α/γ activity for a data set of 71 compounds, compiled from literature. Using Multivariate Data Analysis, satisfactory PLS models were generated for each receptor subtype separately. The models were based on simple and easily interpretable drug‐like and constitutional descriptors, while the inclusion of MOLCONN‐Z descriptors in the initial pool of variables had no considerable impact in model predictivity. By simultaneous analysis of both types of activity, a consensus PLS model for dual PPAR‐α/γ activity could be derived, displaying the molecular features, which may lead to a balanced activity. All models were validated by permutation tests, by dividing the data set into training and test sets, as well as by external validation using a blind test set. Detailed inspection of PPAR‐α and PPAR‐γ crystal structures and molecular simulation supported the differentiation of most important descriptors in the separate PLS models, e.g. the higher impact of lipophilicity and bulk descriptors in PPAR‐α and PPAR‐γ activity respectively, as well as the effect of specific structural descriptors. Molecular simulation provided also explanation for the behavior of certain outliers in the PLS models.
Multiple e‐Pharmacophore Modeling Combined with High‐Throughput Virtual Screening and Docking to Identify Potential Inhibitors of β‐Secretase(BACE1)
Authors: Palakurti Ravichand , Sriram Dharmarajan , Yogeeswari Perumal , Vadrevu Ramakrishna 
Published: April 2013

Abstractβ‐Secretase (BACE1) is an aspartate protease involved in the production of amyloid‐β a major peptide responsible for the pathogenesis of Alzheimer’s disease. Given its role in the formation of amyloids leading to Alzheimer’s disease, it has been a major therapeutic target for intervention and has been a challenge in the past and the progress has been very slow. More than hundred crystal structures with inhibitors are available in the protein data bank. Many strategies for drug design have been employed in the design of numerous diverse ligands for this target and many have failed due to undesirable drug properties primarily the inability to cross the blood‐brain barrier. In the present work we attempted to consider multiple crystal structures with bound inhibitors showing affinity in the range of 2–210 nM efficacy and optimize the pharmacophoric requirement based on the energy involved in binding termed as e‐pharmacophore mapping. A high throughput screening combined with molecular docking, ADMET predictions, logP values and in vitro assay led to the identification of 7 potential compounds showing inhibition at 10µM which could be further developed as novel inhibitors for β‐secretase.
Experimentally and computationally fast method for estimation of a mean kurtosis
Authors: Hansen Brian , Lund Torben E. , Sangill Ryan , Jespersen Sune Nørhøj 
Published: April 2013

AbstractPurposeResults from several recent studies suggest the magnetic resonance diffusion‐derived metric mean kurtosis (MK) to be a sensitive marker for tissue pathology; however, lengthy acquisition and postprocessing time hamper further exploration. The purpose of this study is to introduce and evaluate a new MK metric and a rapid protocol for its estimation.MethodsThe protocol requires acquisition of 13 standard diffusion‐weighted images, followed by linear combination of log diffusion signals, thus avoiding nonlinear optimization. The method was evaluated on an ex vivo rat brain and an in vivo human brain. Parameter maps were compared with MK estimated from a standard diffusion kurtosis imaging (DKI) data set comprising 160 diffusion‐weighted images.ResultsThe new MK displays remarkably similar contrast to MK, and the proposed protocol acquires the necessary data in less than 1 min for full human brain coverage, with a postprocessing time of a few seconds. Scan–rescan reproducibility was comparable with MK.ConclusionThe framework offers a robust and rapid method for estimating MK, with a protocol easily adapted on commercial scanners, as it requires only minimal modification of standard diffusion‐weighting protocols. These properties make the method feasible in practically any clinical setting.
Numerical smoothness and error analysis on WENO for the nonlinear conservation laws
Authors: Sun Tong 
Published: April 2013

AbstractIn this study, we give an a posteriori error analysis on the weighted essentially nonoscillatory schemes for the nonlinear scalar conservation laws. This analysis is based on the new concept of numerical smoothness, with some new error analysis mechanisms developed for the finite difference and finite volume discretizations. The local error estimate is of optimal order in space and time. The global error estimate grows linearly in time, because of the direct application of the L1 ‐contraction between entropy solutions in the error propagation analysis. As a beginning, we only deal with smooth solutions in this article. Within the same error propagation framework, when we deal with piecewise smooth solutions later, we only need to work on estimating the local error where smoothness is lost. The smoothness indicators not only serve the purpose of local error estimation, but also serve as a monitor on both the possible numerical instability and the expected solution shapening. © 2013 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2013
Designed to Disperse
Authors: Adams Geoff , Thöniß Thomas , Gerhard Christoph 
Published: April 2013

AbstractOptical emission spectroscopy is a widely recognised and well‐established tool in a number of different branches of science. In this contribution, simple modelling of imaging prism and grating spectrometers using optical design software as well as a comparison of both principles are presented. Further, basic considerations on the design of such spectrometers and a transfer lens for imaging a given light source onto the spectrometer are presented.
Optics and Lenses — How to Find the Best Fit
Authors: Linkemann Joachim 
Published: April 2013

AbstractHow do you find the right lens for a particular task? What should you consider when selecting the correct connectors? In which way do focal length and f‐number of the lens influence your photographs? The following tutorial explains what you should take into account in terms of optics and lenses to avoid the agony of choice.
Toxic levels of clozapine in a patient with acute urethritis
Authors: Yadav Devender Singh , Singh Sandeep 
Published: April 2013

AbstractAcute infection has been shown in some case reports to cause serum clozapine to rise to toxic levels. Here, Drs Yadav and Singh report the case of a patient with treatment‐resistant schizophrenia who developed toxic levels of serum clozapine during a bout of acute urethritis.