bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection and characterization of Deformed Wing Virus (DWV) in apiaries with stationary and migratory management in the province of Entre Ríos, Argentina
Authors: Gonzalez F. N. , Raticelli F. , Ferrufino C. , Fagúndez G. , Rodriguez G. , Miño S. , Dus Santos M. J. 
Published: July 2024

AbstractIn Argentina, migratory activity in search of floral diversity has become a common approach to maximizing honey production. The Entre Ríos province possesses a floral diversity that allows beekeepers to perform migratory or stationary management. Beyond the impact caused by transhumance, migratory colonies in this province start and end the season in monoculture areas. To study the effect of these practices on viral infection, we assayed for the presence, abundance and genetic characterization of the Deformed Wing Virus (DWV) in honey bees from apiaries with both types of management. In migratory apiaries, DWV was detectable in 86.2% of the colonies at the beginning of the season (September 2018), and 66% at the end of the season (March 2019). On the other hand, DWV was detected in 44.11% and 53.12% of stationary samples, at the beginning and the end of the season, respectively. Sequence analysis from migratory and stationary colonies revealed that all samples belonged to DWV-A type. The highest viral loads were detected in migratory samples collected in September. Higher DWV presence and abundance were associated with migratory management and the sampling time. Based on our findings we propose that the benefit of migration to wild flowering areas can be dissipated when the bee colonies end the season with monoculture.
Prognostic value of platelet levels in patients with aneurysmal Subarachnoid Hemorrhage
Authors: Fischer Igor , Lala Ronahi , Donaldson Daniel M. , Schieferdecker Simon , Hofmann Björn B. , Cornelius Jan Frederick , Muhammad Sajjad 
Published: July 2024

AbstractPathophysiological processes following aneurysmal subarachnoid hemorrhage (aSAH) include upregulated underlying systemic inflammation, which is reflected by changes in different peripheral blood cells and their sub-populations. As inflammation is a crucial process that contributes to post-aSAH complications and clincal outcome, blood cell numbers and ratios in systemic circulation may predict the outcome and provide rapid and easy to quantify point of care biomarkers for these critically ill patients. To identify blood-derived cellular inflammatory parameters which allow a precise prediction of patient outcome after aSAH. In this single-center retrospective study, 19 whole blood-derived cellular inflammatory markers and clinical and demographic parameters for 101 aSAH patients were recorded within 24 h after aSAH. Clinical outcome was quantified with modified Rankin scale (mRS) on discharge. Proportional odds logistic regression (POLR) was used to model the patient outcome as the function of clinical parameters and inflammatory markers. The results were validated on a separate hold-out dataset (220 patients). The on-admission platelet count, mean platelet volume (MPV) and mean platelet volume to platelet ratio (MPR) were found to be significant and predictive of patient outcome on discharge. Mean platelet volume (MPV) and mean platelet volume to platelet ratio (MPR) predicted clinical outcome and may serve as easy to quantify point of care biomarker. The findings are potentially relevant for the management of aSAH.
PIM1 is a potential therapeutic target for the leukemogenic effects mediated by JAK/STAT pathway mutations in T-ALL/LBL
Authors: Lahera Antonio , Vela-Martín Laura , Fernández-Navarro Pablo , Llamas Pilar , López-Lorenzo José L. , Cornago Javier , Santos Javier , Fernández-Piqueras José , Villa-Morales María 
Published: July 2024

AbstractPrecursor T-cell neoplasms (T-ALL/LBL) are aggressive hematological malignancies that arise from the malignant transformation of immature thymocytes. Despite the JAK/STAT pathway is recurrently altered in these neoplasms, there are not pharmacological inhibitors officially approved for the treatment of T-ALL/LBL patients that present oncogenic JAK/STAT pathway mutations. In the effort to identify potential therapeutic targets for those patients, we followed an alternative approach and focused on their transcriptional profile. We combined the analysis of molecular data from T-ALL/LBL patients with the generation of hematopoietic cellular models to reveal that JAK/STAT pathway mutations are associated with an aberrant transcriptional profile. Specifically, we demonstrate that JAK/STAT pathway mutations induce the overexpression of the PIM1 gene. Moreover, we show that the pan-PIM inhibitor, PIM447, significantly reduces the leukemogenesis, as well as the aberrant activation of c-MYC and mTOR pathways in cells expressing different JAK/STAT pathway mutations, becoming a potential therapeutic opportunity for a relevant subset of T-ALL/LBL patients.
Renal Regional Oxygen Saturation and Acute Kidney Injury in Neonates with Perinatal Asphyxia
Authors: Agudelo-Pérez Sergio , Troncoso Gloria , Botero-Rosas Daniel , Muñoz Christian , Rodríguez Andrés , Gómez Andrea Valentina , León Jennifer 
Published: July 2024

Objective Neonates with moderate-to-severe perinatal asphyxia often develop acute kidney injury (AKI). Additionally, therapeutic hypothermia (TH) can affect renal blood flow. This study aimed to evaluate the association between renal regional oxygen saturation (rSrO2) during TH and AKI in neonates with moderate and severe perinatal asphyxia.
Study Design This retrospective longitudinal study included neonates with moderate-to-severe asphyxia who required TH. The primary outcome was the occurrence of AKI, classified as a rate of decrease in creatinine levels of <33% at 72 hours of TH. rSrO2 was continuously monitored by near-infrared spectroscopy during the hypothermia and rewarming phases. Data analysis involved dividing the average rSrO2 levels into 12-hour periods. We analyzed the association between AKI and rSrO2 levels using univariate and multivariate logistic regression models. Furthermore, we assessed the predictive capacity of rSrO2 for AKI by analyzing the area under the receiver operating characteristic curve.
Results Ninety-one patients were included in the study. On average, patients with AKI exhibit lower rSrO2 levels during TH. Specifically, rSrO2 levels within the first 12 hours and between 25 and 72 hours of TH demonstrated the highest predictive capability for AKI. Multivariate logistic regression analysis revealed that rSrO2 levels within the initial 12 hours (adjusted odds ratio [aOR] = 1.11, 95% confidence interval [CI]: 1.01–1.21) and between 61 and 72 hours (aOR = 0.85, 95% CI: 0.78–0.92) were significantly associated with AKI.
Conclusion An increase in rSrO2 during the first 12 hours of TH and lower rSrO2 levels between 61 and 72 hours of treatment were associated with the development of AKI in asphyxiated neonates undergoing TH.
Key Points
Anti-inflammatory Principles of the Plant Family Amaryllidaceae
Authors: Nair Jerald J. , van Staden Johannes 
Published: July 2024

AbstractThere is considerable interest in the utilisation of plants against inflammation. Over 50 species of the plant family Amaryllidaceae are known for such usage in traditional medicine. This review was undertaken to identify the chemical principles responsible for these anti-inflammatory effects. It describes the findings from in vitro, in vivo and in silico studies, as well as the probes made on the mechanisms of action. The literature search returned over 600 hits, of which around 130 were chosen for their relevance to the text. Over 140 compounds have thus far been screened for anti-inflammatory effects. These were mostly isoquinoline alkaloids but also included other classes of secondary metabolites such as chromones, flavonoids and triterpenoids. In vitro studies were carried out in mononuclear cells such as lymphocytes, monocytes, neutrophils and macrophages, against which no serious side effects were observed. The constituents were also effective
against inflammation induced by physical and chemical stimuli in a variety of murine test subjects. Chief among the compounds were the isoquinoline alkaloids lycorine and narciclasine, which displayed potent effects against pain, swelling, asthma and arthritis, amongst others. From a mechanistic perspective, several of the compounds were shown to mediate in inflammatory pathways, notably via the modulation of both pro-inflammatory (such as NF-κB, TNF-α and IL-1) and anti-inflammatory (such as IL-10 and TGF-β) factors. Useful insights also emerged from active-site docking studies of some of the compounds. The Amaryllidaceae affords a rich and diverse platform for the discovery of potential anti-inflammatory drugs.
Exploring Radiology Postgraduate Students' Engagement with Large Language Models for Educational Purposes: A Study of Knowledge, Attitudes, and Practices
Authors: Sarangi Pradosh Kumar , Panda Braja Behari , P. Sanjay , Pattanayak Debabrata , Panda Swaha , Mondal Himel 
Published: July 2024

Abstract
Background The integration of large language models (LLMs) into medical education has received increasing attention as a potential tool to enhance learning experiences. However, there remains a need to explore radiology postgraduate students' engagement with LLMs and their perceptions of their utility in medical education. Hence, we conducted this study to investigate radiology postgraduate students' knowledge, attitudes, and practices regarding LLMs in medical education.
Materials and Methods A cross-sectional quantitative survey was conducted online on Google Forms. Participants from all over India were recruited via social media platforms and snowball sampling techniques. A previously validated questionnaire was used to assess knowledge, attitude, and practices regarding LLMs. Descriptive statistical analysis was employed to summarize participants' responses.
Results A total of 252 (139 [55.16%] males and 113 [44.84%] females) radiology postgraduate students with a mean age of 28.33 ± 3.32 years participated in the study. The majority of the participants (47.62%) were familiar with LLMs with their potential incorporation with traditional teaching–learning tools (71.82%). They are open to including LLMs as a learning tool (71.03%) and think that it would provide comprehensive medical information (62.7%). Residents take the help of LLMs when they do not get the desired information from books (46.43%) or Internet search engines (59.13%). The overall score of knowledge (3.52 ± 0.58), attitude (3.75 ± 0.51), and practice (3.15 ± 0.57) were statistically significantly different (analysis of variance [ANOVA], p < 0.0001), with the highest score in attitude and lowest in practice. However, no significant differences were found in the scores for knowledge (p = 0.64), attitude (p = 0.99), and practice (p = 0.25) depending on the year of training.
Conclusion Radiology postgraduate students are familiar with LLM and recognize the potential benefits of LLMs in postgraduate radiology education. Although they have a positive attitude toward the use of LLMs, they are concerned about its limitations and use it only in limited situations for educational purposes.
Evolutionary game model and simulation analysis of multi-stakeholder behaviour for promoting Braille labelling on pharmaceutical packaging in China’s legal framework
Authors: Feng Zehua , Liu Xiangdong , Zhao Ying , Huang Zhengzong 
Published: July 2024

AbstractThis study investigates the factors influencing the lack of Braille-embossed labels in Chinese pharmaceutical product packaging. To this end, this study constructs an evolutionary game model of multi-participant behaviour in drug production regulation and conducts simulation based on the perspective of stakeholders in order to study the evolutionary steady state of regulatory authorities, pharmaceutical companies, and visually impaired individuals and the influence of each parameter variable on the choice of strategic behaviour of the participants. The simulation results show that in the legal framework of China, administrative penalties, the production subsidy rate, and the Basic Medical Insurance (BMI) reimbursement rate are the main drivers of the system’s evolution towards a steady state. Reduced costs of drugs with Braille-embossed packaging is a prerequisite for the visually impaired to purchase such drugs, and the key for the whole system to converge to a stable state therefore lies in the BMI reimbursement rate for drugs with Braille-embossed packaging. This paper suggests that the Chinese government should improve the law enforcement mechanism for the regulation of the production of drugs with Braille-embossed packaging; increase the type and intensity of administrative penalties as well as production subsidies, tax incentives, and compliance incentives for pharmaceutical companies; and provide higher BMI reimbursement rates for the visually impaired. The results of the study provide valuable insights to improve accessibility to drugs and build a barrier-free environment for ensuring medication safety in China and other developing countries.
The effect of project approach-based science education program on problem-solving skills of preschool children
Authors: Güley Burcu , Keskinkılıç Ayşegül 
Published: July 2024

AbstractIn this study, the effect of project approach-based science “education program on problem-solving skills” of preschool children attending a kindergarten was investigated. The study was conducted with a quasi-experimental design with pretest and posttest control group at a kindergarten affiliated with the “Ministry of National Education” located in a province in Turkey. The study sample consisted of 70 children (35 in the experimental group, 35 in the control group). The study data were collected through Personal Information Form and Age 4–7 “PSSS”. While the Project Approach-Based Science Education Program (PABSEP) was applied to the children in the experimental group in 28 sessions for three days a week for 10 weeks in addition to the National Education Program, the National Education Program was continued in the control group. As a result of the analyses of the data obtained, significant differences were determined between the “PS” scores of the two groups in favor of the experimental group following the education programs applied (p < 0.05). It was concluded that the PABSEP positively affected the problem-solving skills of the preschool children, and that this effect was permanent (p < 0.05).
Field theoretic description of nonlinear electro-optical responses in centrosymmetric electronic systems
Authors: Paul I 
Published: July 2024

Abstract
Motivated by the recent developments in terahertz spectroscopy using pump-probe setups to study correlated electronic materials, we review the field theoretical formalism to compute finite frequency nonlinear electro-optical responses in centrosymmetric systems starting from basic time dependent perturbation theory. We express the nonlinear current kernel as a sum of several causal response functions. These causal functions cannot be evaluated using perturbative field theory methods, since they are not contour ordered. Consequently, we associate each response function with a corresponding imaginary time ordered current correlation function, since the latter can be factorized using Wick’s theorem. The mapping between the response functions and the correlation functions, suitably analytically continued to real frequencies, is proven exactly. We derive constraints satisfied by the nonlinear current kernel and we prove a generalized f-sum rule for the nonlinear conductivity, all of which are consequences of particle number conservation. The constraints guarantee that the nonlinear static responses are free from spurious divergences. We apply the theory to compute the gauge invariant nonlinear conductivity of a system of noninteracting electrons in the presence of weak disorder. As special cases of this generalized nonlinear response, we discuss its third harmonic and its instantaneous terahertz Kerr signals. The formalism can be used to compute the nonlinear conductivity in symmetry broken phases of electronic systems such as superconductors, density waves and nematic states.
Neuromuscular disease auxiliary diagnosis using a portable magnetomyographic system
Authors: Wei Yutong , Chen Yan , Ye Chaofeng 
Published: July 2024

Abstract

Objective. The measurement of electromyography (EMG) signals with needle electrodes is widely used in clinical settings for diagnosing neuromuscular diseases. Patients experience pain during needle EMG testing. It is significant to develop alternative diagnostic modalities. Approach. This paper proposes a portable magnetomyography (MMG) measurement system for neuromuscular disease auxiliary diagnosis. Firstly, the design and operating principle of the system are introduced. The feasibility of using the system for auxiliary diagnosis of neuromuscular diseases is then studied. The magnetic signals and needle EMG signals of thirty subjects were collected and compared. Main results. It is found that the amplitude of muscle magnetic field signal increases during mild muscle contraction, and the signal magnitudes of the patients are smaller than those of normal subjects. The diseased muscles tested in the experiment can be distinguished from the normal muscles based on the signal amplitude, using a threshold value of 6 pT. The MMG diagnosis results align well with the needle EMG diagnosis. In addition, the MMG measurement indicates that there is a persistence of spontaneous activity in the diseased muscle. Significance. The experimental results demonstrate that it is feasible to auxiliary diagnose neuromuscular diseases using the portable MMG system, which offers the advantages of non-contact and painless measurements. After more in-depth, systematic, and quantitative research, the portable MMG could potentially be used for auxiliary diagnosis of neuromuscular diseases. The clinical trial registration number is ChiCTR2200067116.
Estimation of the prevalence of isolated inter-scalene compression from simultaneous arterial and venous photoplethysmography in patients referred for suspected thoracic outlet syndrome
Authors: Lecoq Simon , Hersant Jeanne , Abraham Pierre 
Published: July 2024

Abstract

Objective. In patients with suspected thoracic outlet syndrome (TOS), diagnosing inter-scalene compression could lead to minimally invasive treatments. During photo-plethysmography, completing a 30 s 90° abduction, external rotation (‘surrender’ position) by addition of a 15 s 90° antepulsion ‘prayer’ position, allows quantitative bilateral analysis of both arterial (A-PPG) and venous (V-PPG) results. We aimed at determining the proportion of isolated arterial compression with photo-plethysmography in TOS-suspected patients. Approach. We studied 116 subjects recruited over 4 months (43.3 ± 11.8 years old, 69% females). Fingertip A-PPG and forearm V-PPG were recorded on both sides at 125 Hz and 4 Hz respectively. A-PPG was converted to PPG amplitude and expressed as percentage of resting amplitude (% rest). V-PPG was expressed as percentage of the maximal value (% max) observed during the ‘Surrender-Prayer’ maneuver. Impairment of arterial inflow during the surrender (As+) or prayer (Ap+) phases were defined as a pulse-amplitude either <5% rest, or <25% rest. Incomplete venous emptying during the surrender (Vs+) or prayer (Vp+) phases were defined as V-PPG values either <70% max, or <87% max. Main results. Of the 16 possible associations of encodings, As − Vs − Ap − Vp− was the most frequent observation assumed to be a normal response. Isolated arterial inflow without venous outflow (As + Vs−) impairment in the surrender position was observed in 10.3% (95%CI: 6.7%–15.0%) to 15.1% (95%CI: 10.7%–20.4%) of limbs. Significance. Simultaneous A-PPG and V-PPG can discriminate arterial from venous compression and then potentially inter-scalene from other levels of compressions. As such, it opens new perspectives in evaluation and treatment of TOS.
Investigating the influence of chromium layer thickness on thermal stability, adhesion and agglomeration of Cr/Ag/Cr sandwich layers in microelectromechanical systems (MEMS)
Authors: Ghazavi Hossein , kolahdoozan Mojtaba , Solhjoei Nosratollah , Saadat Mohammad , Mirtalaie Sayed Hasan 
Published: July 2024

Abstract
This study explores the influence of chromium layer thickness on the thermal stability and agglomeration of Cr/Ag/Cr sandwich layers used in MEMS applications. Achieving uniform and consistent deposition of thin films is crucial for optimal device performance. Magnetron sputtering, a technique offering precise control over film properties, is commonly employed for depositing thin films in MEMS. Silver is a popular choice due to its desirable properties, but it tends to agglomerate at high temperatures. The researchers investigated the effect of chromium layer thickness on thermal stability and agglomeration. They deposited chromium layers of varying thicknesses onto silicon substrates, followed by a silver layer and another chromium layer to create a sandwich structure. Annealing was performed at different temperatures to assess thermal stability and prevent silver agglomeration. Thermal stability was evaluated by measuring electrical resistance using a four-point probe method, and surface topography was analyzed using a non-contact atomic force microscope. The goal was to identify the optimal chromium layer thickness to minimize agglomeration and maximize thermal stability. The results showed that a sandwich structure with a 5 nm top chromium layer (Si/Cr (5 nm)/Ag (100 nm)/Cr (5-10-15-20 nm)) exhibited decreased adhesion force with increasing annealing temperatures. The use of a chromium sandwich layer significantly reduced surface roughness, as indicated by reductions in Ra and RMS values. A 15 nm thick chromium layer above and below the silver layer provided the best thermal stability and prevented silver agglomeration, resulting in the highest degree of adhesion. This thickness also yielded optimal surface parameters for the chromium sandwich layers at the annealing temperatures. In conclusion, the study demonstrates that the thickness of the chromium layer influences thermal stability, agglomeration, and surface parameters in MEMS applications and enables better control over thin film deposition.
Characterizing nonclassical correlation via local channels
Authors: Rajendran Muthuganesan , Balakrishnan S 
Published: July 2024

Abstract
Local operation is an important tool to characterize the nonlocal aspects of multipartite quantum system. Exploiting the notion of resource theory of coherence, in this article, we establish a quantum correlation measure as the difference between the bipartite coherence and marginal state coherence. We study the Tsallis α-entropy (TαE) coherence based quantum correlation of bipartite state relative to different channels such as unitary channel, the twirling (unitary-induced) channel, projective measurements and weak measurements. It is shown that the quantum channel helps us to discriminate the product and classical-quantum states. We provide the operational interpretation of the correlation measure relative to the measurement in terms of classical uncertainty of channel. A closer connection between the correlation relative to the projective measurements and weak measurements is obtained in terms of measurement strength. As an illustration, we have studied the quantum correlations of well-known two-qubit states.