bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin temperature measurement using an infrared thermometer on patients who have been exposed to cold
Authors: Erenberk Ufuk , Torun Emel , Ozkaya Emin , Uzuner Selcuk , Demir Aysegul Dogan , Dundaroz Rusen 
Published: August 2013

AbstractBackround The aim of this study was to determine if the skin temperature of febrile children is affected by the child's exposure to cold outdoor temperatures immediately prior to the taking of that temperature.Methods A total of 150 febrile and non‐febrile children (aged 3–10 years) who had walked to the hospital's pediatric emergency department and were thus exposed to outside cold weather were enrolled in the study. Using infrared thermometry, forehead and chest skin temperatures were simultaneously measured every 2 min during the first 14 min after presentation. Temperatures were recorded and differences between the two measurements were calculated.Results By the fifth evaluation (10 min from the first reading), skin temperatures from forehead and chest had equalized.Conclusion Determination of fever from the body parts that had been exposed to cold environmental conditions may cause contradictory results if taken while the child is still chilled from exposure to the cold. For accuracy, children should be acclimated to the indoor temperature before taking body temperature readings. Acclimation takes at least 10 min after coming in from cold weather outside.
Photophysical Properties of Lanthanide (Eu3+, Tb3+) Hybrid Soft Gels of Double Functional Linker of Ionic Liquid–Modified Silane
Authors: Li Qiu‐Ping , Yan Bing 
Published: August 2013

AbstractFour kinds of luminescent hybrid soft gels have been assembled by introducing the lanthanide (Eu3+, Tb3+) tetrakis β‐diketonate into the covalently bonded imidazolium‐based silica through electrostatic interactions. Here, the imidazolium‐based silica matrices are prepared from imidazolium‐derived organotriethoxysilanes by the sol–gel process, in which the imidazolium cations are strongly anchored within the silica matrices while anions can still be exchanged following application for functionalization of lanthanide complexes. The photoluminescence measurements indicated that these hybrid soft gels exhibit characteristic red and green luminescence originating from the corresponding ternary lanthanide ions (Eu3+, Tb3+). Further investigation of photophysical properties reveals that these soft gels have inherited the outstanding luminescent properties from the lanthanide tetrakis β‐diketonate complexes such as strong luminescence intensities, long lifetimes and high luminescence quantum efficiencies.
The influence of microgravity on Euglena gracilis as studied on Shenzhou 8
Authors: Nasir A. , Strauch S. M. , Becker I. , Sperling A. , Schuster M. , Richter P. R. , Weißkopf M. , Ntefidou M. , Daiker V. , An Y. A. , Li X. Y. , Liu Y. D. , Lebert M. 
Published: August 2013

AbstractThe German Aerospace Center (DLR) enabled German participation in the joint space campaign on the unmanned Shenzhou 8 spacecraft in November 2011. In this report, the effect of microgravity on Euglena gracilis cells is described. Custom‐made dual compartment cell fixation units (containing cells in one chamber and fixative – RNA lysis buffer – in another one) were enclosed in a small container and placed in the Simbox incubator, which is an experiment support system. Cells were fixed by injecting them with fixative at different time intervals. In addition to stationary experiment slots, Simbox provides a 1 g reference centrifuge. Cell fixation units were mounted in microgravity and 1 g reference positions of Simbox. Two Simbox incubators were used, one for space flight and the other as ground reference. Cells were fixed soon after launch and shortly before return of the spaceship. Due to technical problems, only early in‐flight samples (about 40 min after launch microgravity and corresponding 1 g reference) were fully mixed with fixative, therefore only data from those samples are presented. Transcription of several genes involved in signal transduction, oxidative stress defence, cell cycle regulation and heat shock responses was investigated with quantitative PCR. The data indicate that Euglena cells suffer stress upon short‐term exposure to microgravity; various stress‐induced genes were up‐regulated. Of 32 tested genes, 18 were up‐regulated, one down‐regulated and the rest remained unaltered. These findings are in a good agreement with results from other research groups using other organisms.
Mesenteric fat thickness is associated with increased risk of obstructive sleep apnoea
Authors: Liu Kin Hung , Chu Winnie C.W. , To Kin Wang , Ko Fanny W.S. , Ng Susanna S.S. , Ngai Jenny C.L. , Chan Jeff Wai Sang , Ahuja Anil T. , Hui David S.C. 
Published: August 2013

AbstractBackground and objectiveMesenteric fat is a type of intraperitoneal adipose tissue draining into portal circulation. The objective of this study was to investigate the relationships between mesenteric fat thickness and obstructive sleep apnoea (OSA) in patients with suspected OSA.MethodsOne hundred forty‐nine subjects (men: 114; women: 35) with suspected OSA underwent ultrasound examinations of mesenteric, preperitoneal and subcutaneous fat thickness after overnight polysomnography. Body mass index (BMI) and neck circumference were recorded.ResultsThe subjects with OSA (n = 130, apnoea/hypopnoea index (AHI) >5/h) had greater neck circumference, higher BMI, and greater mesenteric and preperitoneal fat thickness than those without OSA (n = 19, AHI ≤ 5/h). There was positive correlation of AHI with mesenteric (r = 0.43, P < 0.001) and preperitoneal fat thickness (r = 0.3, P < 0.001), whereas no significant association was observed between AHI and subcutaneous fat thickness (r = 0.09, P = 0.27). On multivariate logistic regression, after adjustments for gender, age, BMI, neck circumference, and preperitoneal and subcutaneous fat thickness, the mesenteric fat thickness had a positive association with the presence of moderate OSA and severe OSA, with odds ratios of 7.18 and 7.45 for every 1 cm increase in mesenteric fat thickness when AHI was defined as ≥15/h and AHI ≥ 30/h, respectively.ConclusionsMesenteric fat thickness is associated with increased risk of OSA, independent of other abdominal fat thickness, BMI and neck circumference. Sonographic measurement is potentially a useful tool for further evaluating the complex association of visceral fat, metabolic syndrome and OSA.
Closing the Wealth Gap: A Review of Racial and Ethnic Inequalities in Homeownership
Authors: Kuebler Meghan 
Published: August 2013

AbstractHomeownership plays an important role in the socioeconomic well‐being of Americans. Despite recent major losses in wealth due to the subprime market crash, home equity remains the largest source of wealth for the average American family. A marker of class status, owning one's home provides access to neighborhoods with the best schools, quality public services, and lowest crime. This article demonstrates that minorities have not had the same access to homeownership that Whites have, and this contributes to continuing socioeconomic disparities between Whites and minorities. This article explores the homeownership experience of Blacks – including African‐Americans, Caribbeans, and Africans – Hispanics, and Asians in the United States relative to non‐Hispanic Whites. Minorities rely heavily on homeownership and home equity as the key component of their wealth and remain less likely than Whites to hold alternative forms of investment such as stocks. The role that homeownership plays in perpetuating intergenerational wealth disparities between Whites and minorities is discussed as are challenges to minority homeownership such as the pervasiveness of risky mortgage products. Exploring the racial gap in homeownership is fundamental to understanding racial inequalities and formulating strategies and policies to help close such disparities. This article concludes with suggestions for future research.
Racialization and Muslims: Situating the Muslim Experience in Race Scholarship
Authors: Selod Saher , Embrick David G. 
Published: August 2013

AbstractThis article reviews how racialization enables an understanding of Muslim and Muslim American experience as racial. Race scholarship in the United States has historically been a Black/White paradigm. As a result, the experiences of many racial and ethnic groups who have become a part of the American landscape due to the passage of the Immigration and Nationality Act of 1965 have largely been ignored in race scholarship. By reviewing racialization and its application to Arabs and Muslims, it is apparent that scholars must continuously explore newer theories and languages of race. Racialization not only provides a way to understand the fluidity of race and racism but it also contributes to the advancement of race scholarship by reflecting on the current contextual influences on race.
Associations Between Behavioral Inhibition and Children's Social Problem‐solving Behavior During Social Exclusion
Authors: Walker Olga L. , Henderson Heather A. , Degnan Kathryn A. , Penela Elizabeth C. , Fox Nathan A. 
Published: August 2013

AbstractThe current study examined the associations between the early childhood temperament of behavioral inhibition and children's displays of social problem‐solving (SPS) behavior during social exclusion. During toddlerhood (the ages of two to three), maternal report and behavioral observations of behavioral inhibition were collected. At the age of seven, children's SPS behaviors were observed during a laboratory social exclusion task based on the commonly used Cyberball game. Results showed that behavioral inhibition was positively associated with displayed social withdrawal and negatively associated with assertive behavior during the observed social exclusion task at seven years of age. These results add to our understanding of inhibited children's SPS behaviors during social exclusion and provide evidence for the associations between toddler temperament and children's social behavior during middle childhood.
Soil structural degradation in SW England and its impact on surface‐water runoff generation
Authors: Palmer R. C. , Smith R. P. 
Published: August 2013

AbstractField investigations between 2002 and 2011 identified soil structural degradation to be widespread in SW England with 38% of the 3243 surveyed sites having sufficiently degraded soil structure to produce observable features of enhanced surface‐water runoff within the landscape. Soil under arable crops often had high or severe levels of structural degradation. Late‐harvested crops such as maize had the most damaged soil where 75% of sites were found to have degraded structure generating enhanced surface‐water runoff. Soil erosion in these crops was found at over one in five sites. A tendency for the establishment of winter cereals in late autumn in the South West also often resulted in damaged soil where degraded structure and enhanced surface‐water runoff were found in three of every five cereal fields. Remedial actions to improve soil structure are either not being undertaken or are being unsuccessfully used. Brown Sands, Brown Earths and loamy Stagnogley Soils were the most frequently damaged soils. The intensive use of well‐drained, high quality sandy and coarse loamy soils has led to soil structural damage resulting in enhanced surface‐water runoff from fields that should naturally absorb winter rain. Surface water pollution, localized flooding and reduced winter recharge rates to aquifers result from this damage. Chalk and limestone landscapes on the other hand show little evidence of serious soil structural degradation and <20% of fields in these landscapes generate enhanced runoff.
Autologous skin substitute for hard‐to‐heal ulcers: Retrospective analysis on safety, applicability, and efficacy in an outpatient and hospitalized setting
Authors: Blok Chantal S. , Vink Liselot , de Boer Edith M. , van Montfrans Catherine , van den Hoogenband Henk M. , Mooij Michael C. , Gauw Stefanie A. , Vloemans Jos A. F. P. M , Bruynzeel Ineke , van Kraan Aleid , Kuik Joop , Waaijman Taco , Scheper Rik J. , Gibbs Susan 
Published: August 2013

AbstractChronic ulcers ((arterio)venous, decubitus, or postoperative) have no tendency to heal within a period of at least 3 months despite optimal therapy according to internationally accepted guidelines. This retrospective study evaluates the safety and efficacy of an autologous, dermal‐epidermal skin substitute (SS) for treating ulcers of various origins. Ulcers were treated within 7 Dutch centers over 5 years. Sixty‐six ulcers (size: 0.75–150 cm2; duration: 0.25–32 years) with a follow‐up time of 24 weeks after a single‐skin substitute application were assessed. Wound‐bed preparation consisted of vacuum‐assisted‐closure‐therapy (5 days, hospitalized) or application of acellular dermis (5–7 days, outpatient). Time to heal, adverse events, and recurrence 1 year after complete healing were recorded. Complete ulcer healing occurred in 36 of 66 ulcers (55%) at 24 weeks. At that time point, a further 29% of ulcers showed decrease in ulcer size between 50 and 99%. No difference was observed between the hospitalized vs. outpatient treatment with complete healing. There were 32 of 36 healed ulcers that were available for follow‐up 1 year after complete closure, of which 27 (84%) were still closed. Only two minor/moderate possibly related adverse events were recorded. This retrospective analysis shows that SS provides a safe and successful treatment for particularly chronic ulcers of various origins.
Modified Opsonization, Phagocytosis, and Killing Assays To Measure Potentially Protective Antibodies against Pneumococcal Surface Protein A
Authors: Daniels Calvin C. , Kim Kyung-Hyo , Burton Robert L. , Mirza Shaper , Walker Melissa , King Janice , Hale Yvette , Coan Patricia , Rhee Dong-Kwon , Nahm Moon H. , Briles David E. 
Published: August 2013

ABSTRACTThe standard opsonophagocytosis killing assay (OPKA) for antibodies to pneumococcal capsular polysaccharide was modified to permit an evaluation of the protection-mediating antibodies to pneumococcal surface protein A (PspA). We found that by increasing the incubation time with the complement and phagocytes from 45 min to 75 min, the protective activity was readily detected. In another modification, we used a capsule type 2 target strain that expressed PspA but not pneumococcal surface protein C (PspC). With these modifications separately or in combination, rabbit antisera to the recombinant α-helical or proline-rich domains of PspA mediated >50% killing of the target strain. The ability of normal human sera to mediate the killing of pneumococci in this modified OPKA correlated with their levels of antibodies to PspA and their ability to protect mice against fatal infection with a type 3 strain. Passive protection of mice against pneumococci and killing in the modified OPKA were lost when normal human sera were adsorbed with recombinant PspA (rPspA) on Sepharose, thus supporting the potential utility of the modified OPKA to detect protective antibodies to PspA. In the standard OPKA, monoclonal antibodies to PspA were strongly protective in the presence of subprotective amounts of anti-capsule. Thus, the currently established high-throughput OPKA for antibodies to capsule could be modified in one of two ways to permit an evaluation of the opsonic efficacy of antibodies to PspA.
Subcutaneous Administration of a 10-Fold-Lower Dose of a Commercial Human Tuberculosis Vaccine, Mycobacterium bovis Bacillus Calmette-Guérin Danish, Induced Levels of Protection against Bovine Tuberculosis and Responses in the Tuberculin Intradermal Test Similar to Those Induced by a Standard Cattle Dose
Authors: Buddle Bryce M. , Hewinson R. Glyn , Vordermeier H. Martin , Wedlock D. Neil 
Published: August 2013

ABSTRACTVaccination of cattle with a commercial human tuberculosis (TB) vaccine,Mycobacterium bovisbacillus Calmette-Guérin (BCG) Danish, at a dose equivalent to 5 human doses of BCG has protected these animals against TB in field and experimental trials. There is interest in determining whether a 10-fold-lower dose could still protect cattle but not induce a tuberculin intradermal test response. Two groups of calves (n= 9/group) were vaccinated subcutaneously with a lyophilized BCG Danish vaccine containing either 0.5 (1 × 105to 4 × 105CFU) or 5 (1 × 106to 4 × 106CFU) human doses of BCG Danish, with an additional group of 10 calves serving as nonvaccinated controls. Fifteen weeks after vaccination, these animals were challenged intratracheally with 5 × 103CFU of virulentM. bovisand another 15 weeks later were slaughtered and examined for the presence of tuberculous lesions. Vaccination of the calves with either 0.5 or 5 equivalent human doses of BCG Danish induced similar levels of protection against challenge withM. bovis, with both groups showing significant reductions in the pathological and microbiological parameters compared to those for the the control group (P< 0.05). Vaccination with either of the two BCG doses induced similar numbers of animals responding to the tuberculin intradermal test at 11 weeks postvaccination. Vaccination with a 0.5 equivalent human dose of a commercial lyophilized BCG vaccine can protect cattle against challenge withM. bovis.
The Golgi Protein ACBD3, an Interactor for Poliovirus Protein 3A, Modulates Poliovirus Replication
Authors: Téoulé François , Brisac Cynthia , Pelletier Isabelle , Vidalain Pierre-Olivier , Jégouic Sophie , Mirabelli Carmen , Bessaud Maël , Combelas Nicolas , Autret Arnaud , Tangy Frédéric , Delpeyroux Francis , Blondel Bruno 
Published: August 2013

ABSTRACT
We have shown that the circulating vaccine-derived polioviruses responsible for poliomyelitis outbreaks in Madagascar have recombinant genomes composed of sequences encoding capsid proteins derived from poliovaccine Sabin, mostly type 2 (PVS2), and sequences encoding nonstructural proteins derived from other human enteroviruses. Interestingly, almost all of these recombinant genomes encode a nonstructural 3A protein related to that of field coxsackievirus A17 (CV-A17) strains. Here, we investigated the repercussions of this exchange, by assessing the role of the 3A proteins of PVS2 and CV-A17 and their putative cellular partners in viral replication. We found that the Golgi protein acyl-coenzyme A binding domain-containing 3 (ACBD3), recently identified as an interactor for the 3A proteins of several picornaviruses, interacts with the 3A proteins of PVS2 and CV-A17 at viral RNA replication sites, in human neuroblastoma cells infected with either PVS2 or a PVS2 recombinant encoding a 3A protein from CV-A17 [PVS2-3A(CV-A17)]. The small interfering RNA-mediated downregulation of ACBD3 significantly increased the growth of both viruses, suggesting that ACBD3 slowed viral replication. This was confirmed with replicons. Furthermore, PVS2-3A(CV-A17) was more resistant to the replication-inhibiting effect of ACBD3 than the PVS2 strain, and the amino acid in position 12 of 3A was involved in modulating the sensitivity of viral replication to ACBD3. Overall, our results indicate that exchanges of nonstructural proteins can modify the relationships between enterovirus recombinants and cellular interactors and may thus be one of the factors favoring their emergence.
Limited Nucleotide Changes in the Rev Response Element (RRE) during HIV-1 Infection Alter Overall Rev-RRE Activity and Rev Multimerization
Authors: Sloan Emily A. , Kearney Mary F. , Gray Laurie R. , Anastos Kathryn , Daar Eric S. , Margolick Joseph , Maldarelli Frank , Hammarskjold Marie-Louise , Rekosh David 
Published: August 2013

ABSTRACT

HIV-1 Rev and the Rev response element (RRE) enable a critical step in the viral replication cycle by facilitating the nuclear export of intron-containing mRNAs, yet their activities have rarely been analyzed in natural infections. This study characterized their genetic and functional variation in a small cohort of HIV-infected individuals. Multiple Rev and RRE sequences were obtained using single-genome sequencing (SGS) of plasma samples collected within 6 months after seroconversion and at a later time. This allowed the identification of cognate sequences that were linked
in vivo
in the same viral genome and acted together as a functional unit. Phylogenetic analyses of these sequences indicated that 4/5 infections were founded by a single transmission event. Rev and RRE variants from each time point were subjected to functional analysis as both cognate pairs and as individual components. While a range of Rev-RRE activities were seen, the activity of cognate pairs from a single time point clustered to a discrete level, which was termed the set point. In 3/5 patients, this set point changed significantly over the time period studied. In all patients, RRE activity was more sensitive to sequence variation than Rev activity and acted as the primary driver of the cognate set point. Selected patient RREs were also shown to have differences in Rev multimerization using gel shift binding assays. Thus, rather than acting as a simple on-off switch or maintaining a constant level of activity throughout infection, the Rev-RRE system can fluctuate, presumably to control replication.
Compensatory Hemagglutinin Mutations Alter Antigenic Properties of Influenza Viruses
Authors: Myers Jaclyn L. , Wetzel Katherine S. , Linderman Susanne L. , Li Yang , Sullivan Colleen B. , Hensley Scott E. 
Published: August 2013

ABSTRACT
Influenza viruses routinely acquire mutations in antigenic sites on the globular head of the hemagglutinin (HA) protein. Since these antigenic sites are near the receptor binding pocket of HA, many antigenic mutations simultaneously alter the receptor binding properties of HA. We previously reported that a K165E mutation in the Sa antigenic site of A/Puerto Rico/8/34 (PR8) HA is associated with secondary neuraminidase (NA) mutations that decrease NA activity. Here, using reverse genetics, we show that the K165E HA mutation dramatically decreases HA binding to sialic acid receptors on cell surfaces. We sequentially passaged reverse-genetics-derived PR8 viruses with the K165E antigenic HA mutation in fertilized chicken eggs, and to our surprise, viruses with secondary NA mutations did not emerge. Instead, viruses with secondary HA mutations emerged in 3 independent passaging experiments, and each of these mutations increased HA binding to sialic acid receptors. Importantly, these compensatory HA mutations were located in the Ca antigenic site and prevented binding of Ca-specific monoclonal antibodies. Taken together, these data indicate that HA antigenic mutations that alter receptor binding avidity can be compensated for by secondary HA or NA mutations. Antigenic diversification of influenza viruses can therefore occur irrespective of direct antibody pressure, since compensatory HA mutations can be located in distinct antibody binding sites.
Subviral Dense Bodies of Human Cytomegalovirus Stimulate Maturation and Activation of Monocyte-Derived Immature Dendritic Cells
Authors: Sauer Caroline , Klobuch Sebastian , Herr Wolfgang , Thomas Simone , Plachter Bodo 
Published: August 2013

ABSTRACT
Dendritic cells play a central role in the immune control of human cytomegalovirus (HCMV) infection. This work aimed at investigating the impact of noninfectious, subviral dense bodies of HCMV on the maturation and activation of dendritic cells (DC). Treatment of immature DC with dense bodies led to the maturation of these cells and significantly increased their capacity for cytokine release and antigen presentation. Dense body-activated DC may thereby contribute to the development of antiviral immunity.