bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microstructure and biocompatibility of composite biomaterials fabricated from titanium and tricalcium phosphate by spark plasma sintering
Authors: Mondal Dibakar , Nguyen Linh , Oh Ik‐Hyun , Lee Byong‐Taek 
Published: November 2012

AbstractImportant issues in developing hydroxyapatite (HAp)‐ and titanium (Ti)‐based composite biomaterials for orthopedic or dental devices include the dissociation of HAp during fabrication and its influences in the microstructure and biocompatibility of the final composite. During the densification by sintering of HAp/Ti composites, Ti reacts with OH freed from HAp to form TiO2 thus dissociated HAp into Ca3(PO4)2, CaO, CaTiO3, TiP, and so forth. To inhibit this reaction, composites were fabricated with Ti and 30, 50, and 70 vol % β‐tricalcium phosphate (β‐TCP) instead of HAp by spark plasma sintering at 1200°C. It has been observed that after sintering at 1200°C, Ti also reacted with TCP, but unlike HAp/Ti composites, the final TCP/Ti composites contained significant amounts of unreacted TCP and Ti phases. The initial 70 vol % TCP/Ti composite showed compressive strength of 388.5 MPa, Young's modulus of 3.23 GPa, and Vickers hardness of 361.9 HV after sintering. The in vitro cytotoxicity and proliferation of osteoblast cells on the composites surfaces showed that the addition of a higher amount of TCP with Ti was beneficial by increasing cell viability, cell–composite attachment and proliferation. Osteopontin and collagen type II protein expression from osteoblasts cultured onto the 70% TCP–Ti composite was also higher than other composites and pure Ti. In vivo study verified that within 3 months of implantation in an animal body, 70% TCP–Ti had an excellent bone–implant interface compared with a pure Ti metal implant. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
MRT Letter: Application of novel “in vivo cryotechnique” in living animal kidneys
Authors: LI Zilong , Li Kai , Wang Juan , Zhai Xiaoyue , Wang Lining , Ohno Nobuhiko , Ohno Shinichi 
Published: November 2012

AbstractAim:To compare the influence of different fixation procedures on morphologic studies in living mice, and to identify the advantages of the “in vivo cryotechnique” (IVCT).Methods:We prepared mouse kidneys using four different fixation methods: conventional immersion‐fixation, quick‐freezing following resection of the kidney, quick‐freezing following perfusion‐fixation, and IVCT.Results:Kidney glomeruli were noticeably contracted after conventional immersion‐fixation or quick‐freezing following resection compared to glomeruli from tissues preserved by the IVCT. With the IVCT, both albumin and IgG were colocalized exclusively along or within the glomerular capillary loops; however, immunoreactivity of these proteins in the other three methods was clearly detected in the Bowman's space and apical cytoplasm of the proximal tubules. With the IVCT, immunoreactivity of collagen type IV was very weak at the glomerular basement membrane (GBM) until microwave treatment, which increased its immunoreactivity. In contrast, the immunoreactivity was clearly detected at the GBM with or without microwave treatment with quick‐freezing following perfusion‐fixation. With quick‐freezing following perfusion‐fixation, aquaporin‐1 (AQP‐1) was irregularly distributed in a disorganized manner on the brush border and apical cell membrane along the proximal tubules. But AQP‐1 was labeled intensely and regularly along the brush border and apical cell membrane andonly weakly along the basolateral membrane of the proximal tubules with the IVCT.Conclusion:The IVCT may reliably maintain soluble serum proteins and renal intrinsic proteins such as AQP‐1 in situ and capture transient structures and functional changes in vivo. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.
Rapid and simple immunochemical screening combined with hand‐shaking extraction for thiamethoxam residue in agricultural products
Authors: Watanabe Eiki , Kobara Yuso , Miyake Shiro 
Published: November 2012

AbstractBackgroundWith the aim of expanding the applicability of a kit‐based enzyme‐linked immunosorbent assay (ELISA) for the neonicotinoid insecticide thiamethoxam, the ELISA was newly applied to three kinds of agricultural samples (green pepper, eggplant and spinach). To offer the ELISA as a screening analysis for thiamethoxam residues, a rapid and simple method of extraction by hand‐shaking was used, and speed‐up and simplification of the sample treatment before the ELISA analysis were examined. Finally, the validity of the ELISA combined with the proposed extraction method was verified against a reference high‐performance liquid chromatography (HPLC) method using real‐world agricultural samples.ResultsThere were no marked matrix effects derived from green pepper and eggplant extracts. On the other hand, although the effect due to a pigment in spinach extract on the assay performance was significant, it was effectively avoided by increasing the dilution level of the spinach extract. For thiamethoxam‐spiked samples, acceptable recoveries of 97.9–109.1% and coefficients of variation of 0.3–11.5% were obtained. Inspection of the validity of the ELISA by comparison with the reference HPLC method showed that the two analytical results were very similar, and a high correlation was found between them (r > 0.997).ConclusionThe evaluated ELISA combined with hand‐shaking extraction provided a rapid and simple screening analysis that was quantitative and reliable for the detection of thiamethoxam in complex agricultural products.© 2012 Society of Chemical Industry
Simultaneous determination of bisphenol A, tetrabromobisphenol A, and perfluorooctanoic acid in small household electronics appliances of “Prohibition on Certain Hazardous Substances in Consumer Products” instruction using ultra‐performance liquid chromatography‐tandem mass spectrometry with accelerated solvent extraction
Authors: Guo Qiaozhen , Du Zhenxia , Zhang Yun , Lu Xiaoyu , Wang Jinhua , Yu Wenlian 
Published: November 2012

Simultaneous determination of bisphenol A, tetrabromobisphenol A, and perfluorooctanoic acid in small household electronics appliances by accelerated solvent extraction‐ultra‐performance liquid chromatography‐tandem mass spectrometry was established. Samples, heated for 5 min, were extracted by toluene/methanol (10:1, v/v) under the pressure 1500 psi at 100°C, and were extracted 3 static cycles with 20 min per cycle. And then 15 mL extractant solvent was used to wash the samples, and at last the sample was purged by nitrogen for 100 s. The partial extractant (10 mL) was concentrated by nitrogen and re‐dissolved with 1 mL methanol/water (1:1, v/v). The three compounds were separated by BEH C18 column effectively in 3 min and detected by electrospray ionization mode mass spectrometry. The linear ranges for bisphenol A, perfluorooctanoic acid, and tetrabromobisphenol A were 1–100, 10–1000 ng/mL, and 0.1–10 μg/mL, respectively. The correlation coefficient was greater than 0.996. The LOD and limit of quantitation for three compounds were 0.1, 10, 1 ng/mL, and 0.5, 50, 5 ng/mL, respectively. And the recoveries were 84–92, 76–82, and 72–74%, respectively, with RSD < 5%. The method was successfully used in determining the real samples. The method and the result were confirmed by liquid chromatography‐ion trap‐time of flight mass spectrometry.
Accuracy assessment of detection performance for sidescan sonars
Authors: Nguyen Paul , Murdoch Duncan 
Published: November 2012

AbstractSidescan sonars are used in detecting hostile targets on the seabed. An accurate representation of the sensor performance is required to carefully plan and execute searches. We studied several confidence interval methods for assessing the accuracy of detection performance as a function of range from hostile targets. Using simulation, we determined that the best approach for modeling sensor data was piecewise logistic regression with cubic B‐splines at carefully selected knots. This method was demonstrated on real data collected from the CITADEL sea trial. © 2012 Wiley Periodicals, Inc. Naval Research Logistics, 2012
Stein–Chen approximation and error bounds for order fill rates in assemble‐to‐order systems
Authors: Zhou Wenhui , Chao Xiuli 
Published: November 2012

AbstractAssemble‐to‐order (ATO) is an important operational strategy for manufacturing firms to achieve quick response to customer orders while keeping low finished good inventories. This strategy has been successfully used not only by manufacturers (e.g., Dell, IBM) but also by retailers (e.g., Amazon.com). The evaluation of order‐based performance is known to be an important but difficult task, and the existing literature has been mainly focused on stochastic comparison to obtain performance bounds. In this article, we develop an extremely simple Stein–Chen approximation as well as its error‐bound for order‐based fill rate for a multiproduct multicomponent ATO system with random leadtimes to replenish components. This approximation gives an expression for order‐based fill rate in terms of component‐based fill rates. The approximation has the property that the higher the component replenishment leadtime variability, the smaller the error bound. The result allows an operations manager to analyze the improvement in order‐based fill rates when the base‐stock level for any component changes. Numerical studies demonstrate that the approximation performs well, especially when the demand processes of different components are highly correlated; when the components have high base‐stock levels; or when the component replenishment leadtimes have high variability. © 2012 Wiley Periodicals, Inc. Naval Research Logistics, 2012
Predictors of anxiety and depression in hematopoietic stem cell transplant patients during protective isolation
Authors: Tecchio Cristina , Bonetto Chiara , Bertani Mariaelena , Cristofalo Doriana , Lasalvia Antonio , Nichele Ilaria , Bonani Anna , Andreini Angelo , Benedetti Fabio , Ruggeri Mirella , Pizzolo Giovanni 
Published: November 2012

AbstractObjectiveTo examine in a sample of hematopoietic stem cell transplant patients assessed throughout protective isolation (i) levels of anxiety and depression and (ii) pre‐isolation factors (socio‐demographics, biomedical variables and personality traits), which might predict higher levels of anxiety and depression during isolation.MethodsThe study used a longitudinal prospective design. Anxiety and depression were assessed in 107 participants by the State‐Trait Anxiety Inventory and Self‐rating Depression Scale at admission and weekly at fixed time points throughout isolation. Among pre‐isolation factors, patients' psychological status was evaluated by the Cognitive Behavioral Assessment (2.0). Predictors were explored by random‐effects models.ResultsOne‐tenth of the patients suffered from clinically significant anxiety and depressive symptoms at admission. Although the percentage of depressed patients increased more than twofold after 2 weeks of isolation, that of anxious patients did not significantly change over time. Female gender, higher anxiety and obsessive–compulsive symptoms, intratensive personality traits and lower performance status predicted higher depression during isolation.ConclusionsAnxiety and depression represent a relevant problem for hematopoietic stem cell transplant patients during isolation. Early detection of predictors, such as anxiety levels, obsessive–compulsive symptoms and performance status, could help prevent depression via targeted psychological intervention. Copyright © 2012 John Wiley & Sons, Ltd.
Body image in recently diagnosed young women with early breast cancer
Authors: Rosenberg Shoshana M. , Tamimi Rulla M. , Gelber Shari , Ruddy Kathryn J. , Kereakoglow Sandra , Borges Virginia F. , Come Steven E. , Schapira Lidia , Winer Eric P. , Partridge Ann H. 
Published: November 2012

AbstractObjectiveTo assess body image concerns among young women following a breast cancer diagnosis.MethodsA total of 419 women with recently diagnosed stage 0–III breast cancer were surveyed following enrollment as part of a prospective cohort study of women age 40 or younger at diagnosis. Body image was assessed using three items from the psycho‐social scale of the Cancer Rehabilitation Evaluation System (CARES). CARES scores range from 0 to 4, with higher scores indicative of greater image concerns. Mean CARES scores were calculated and compared between treatment groups using t‐tests and analysis of variance. Multiple linear regression models were fit to evaluate the relationship between physical and psychological factors and body image.ResultsMean time from diagnosis to completion of the baseline survey was 5.2 months. The mean CARES score for all women was 1.28. Mean CARES scores in the mastectomy‐only group (1.87) and in the mastectomy with reconstruction group (1.52) were significantly higher (p < 0.0001) compared with the scores in the lumpectomy group (0.85), indicating that radical surgery was associated with more body image concerns. Radiation (p = 0.01), anxiety (p = 0.0001), depression (p < 0.0001), fatigue (p = 0.04), musculoskeletal pain symptoms (p < 0.0001), weight gain (p = 0.01), and weight loss (p = 0.02), in addition to surgery type (p < 0.0001), were all associated with more body image concerns in the multi‐variable analysis.ConclusionThis analysis highlights the impact of treatment, along with physical and psychological factors, on body image early in the survivorship period. Our findings provide targets for potential future intervention and may aid young women in the surgical decision‐making process. Copyright © 2012 John Wiley & Sons, Ltd.
Elaborate Design Strategies Toward Novel Microcarriers for Controlled Encapsulation and Release
Authors: Shim Tae Soup , Kim Shin‐Hyun , Yang Seung‐Man 
Published: November 2012

AbstractMicroencapsulation and the controlled release of bioactive agents have long been investigated and exploited to both improve the fundamental understanding of cell functionality and to develop efficient delivery vehicles for drugs, nutrients, and cosmetics. Conventional approaches to the synthesis of particles and capsules for practical applications have achieved only limited control over particle size, shape, functionality, and encapsulation efficiency. To overcome such limitations, a variety of approaches have been developed. Recent advances in microfluidics and other techniques have inspired the design of new microcarriers that efficiently encapsulate bioactive agents to enable the on‐demand release or functionalization of encapsulants. Here, the current state of the art in the area of elaborate design strategies for microcarriers that enable efficient encapsulation and controlled release for biological applications is described. This is discussed in three sections, which are categorized by microcarrier type: particle‐type carriers, capsule‐type carriers, and foldable microcarriers. In each section, new routes to fabricating microcarriers are discussed together with their functionalities; techniques based on droplet microfluidics, lithography, micromolding, and imprinting are covered. In addition, the synthetic routes and the microcarriers are evaluated by comparison with alternative routes. Finally, future perspectives for these new strategies are outlined briefly.
Selection of a fungal isolate for the control of the pink hibiscus mealybug Maconellicoccus hirsutus
Authors: Ibarra‐Cortés Karla H , Guzmán‐Franco Ariel W , González‐Hernández Héctor , Suarez‐Espinosa Javier , Baverstock Jason 
Published: November 2012

AbstractBackgroundMaconellicoccus hirsutus Green is a widely distributed pest of numerous crops. Although synthetic pesticides are used to control this pest, entomopathogenic fungi may provide an alternative control mechanism. Three experiments were carried out to select a fungal isolate with the potential to be used as a microbial control agent. The in vitro growth of five isolates of Beauveria bassiana sensu lato (Bals.) Vuill and Metarhizium anisopliae sensu lato (Metschn.) Sorokín, along with three isolates of Lecanicillium lecanii (Zimm.) Zare & W. Gams and Isaria fumosoroseus (Wize), was assessed at four temperatures. The in vivo sporulation of eight selected isolates was then evaluated, followed by the susceptibility of third‐instar M. hirsutus to a single dose (1 × 108 conidia mL−1) of each of these isolates.ResultsGrowth was greatest by isolates of I. fumosoroseus and L. lecanii at 15 and 20 °C and by isolates of M. anisopliae at 25 and 30 °C. In vivo conidium production was greatest when infected with B. bassiana isolate GHA and M. anisopliae isolate Ma65. Mortality was greatest when inoculated with M. anisopliae isolates Ma65 and Ma129.ConclusionIsolate Ma65 shows the best potential to be developed as a microbial control agent for M. hirsutus.© 2012 Society of Chemical Industry
Stanford R. Ovshinsky (1922–2012)
Authors: Kolobov Alexander V. 
Published: November 2012

AbstractThe October 2012 issue of physica status solidi (b) was dedicated to the 90th birthday of Stanford Robert Ovshinsky. After the publication of the online version of the special issue , sad news came: on 17 October 2012, Stan Ovshinsky passed away, a month short of his 90th anniversary.
Emerging viral diseases in kidney transplant recipients
Authors: Moal Valérie , Zandotti Christine , Colson Philippe 
Published: November 2012

SUMMARYViruses are the most important cause of infections and a major source of mortality in Kidney Transplant Recipients (KTRs). These patients may acquire viral infections through exogenous routes including community exposure, donor organs, and blood products or by endogenous reactivation of latent viruses. Beside major opportunistic infections due to CMV and EBV and viral hepatitis B and C, several viral diseases have recently emerged in KTRs. New medical practices or technologies, implementation of new diagnostic tools, and improved medical information have contributed to the emergence of these viral diseases in this special population.The purpose of this review is to summarize the current knowledge on emerging viral diseases and newly discovered viruses in KTRs over the last two decades. We identified viruses in the field of KT that had shown the greatest increase in numbers of citations in the NCBI PubMed database. BKV was the most cited in the literature and linked to an emerging disease that represents a great clinical concern in KTRs. HHV‐8, PVB19, WNV, JCV, H1N1 influenza virus A, HEV, and GB virus were the main other emerging viruses. Excluding HHV8, newly discovered viruses have been infrequently linked to clinical diseases in KTRs. Nonetheless, pathogenicity can emerge long after the discovery of the causative agent, as has been the case for BKV. Overall, antiviral treatments are very limited, and reducing immunosuppressive therapy remains the cornerstone of management. Copyright © 2012 John Wiley & Sons, Ltd.
Short Multiwall Carbon Nanotubes Promote Neuronal Differentiation of PC12 Cells via Up‐Regulation of the Neurotrophin Signaling Pathway
Authors: Meng Li , Chen Rui , Jiang Aihua , Wang Liming , Wang Peng , Li Chen‐zhong , Bai Ru , Zhao Yuliang , Autrup Herman , Chen Chunying 
Published: November 2012

AbstractNumerous unique properties of carbon nanotubes make them attractive for applications in neurobiology such as drug delivery, tissue regeneration, and as scaffolds for neuronal growth. In this study, the critical roles of the length of multiwall carbon nanotubes (MWCNTs) on a neuronal‐like model cell line PC12 cells are investiaged. Incubation of PC12 cells with carboxylated MWCNTs did not significantly affect cellular morphology and viability at lower concentrations. Short MWCNTs show higher cellular uptake and more obvious removal compared to longer ones, which can result in higher ability to promote PC12 cell differentiation. Pre‐incubation of short MWCNTs can up‐regulate the expression of neurotrophin signaling pathway‐associated TrkA/p75 receptors and Pincher/Gap43/TH proteins, which might be the underlying mechanism for the improved differentiation in PC12 cells. The current results provide insight for future applications of MWCNTs in neuron drug delivery and neurodegenerative disease treatment.
Tie2+ Bone Marrow Endothelial Cells Regulate Hematopoietic Stem Cell Regeneration Following Radiation Injury
Authors: Doan Phuong L. , Russell J. Lauren , Himburg Heather A. , Helms Katherine , Harris Jeffrey R. , Lucas Joseph , Holshausen Kirsten C. , Meadows Sarah K. , Daher Pamela , Jeffords Laura B. , Chao Nelson J. , Kirsch David G. , Chute John P. 
Published: November 2012

Abstract
Hematopoietic stem cells (HSCs) reside in proximity to bone marrow endothelial cells (BM ECs) and maintenance of the HSC pool is dependent upon EC-mediated c-kit signaling. Here, we used genetic models to determine whether radioprotection of BM ECs could facilitate hematopoietic regeneration following radiation-induced myelosuppression. We developed mice bearing deletion of the proapoptotic proteins, BAK and BAX, in Tie2+ ECs and HSCs (Tie2Bak/BaxFl/− mice) and compared their hematopoietic recovery following total body irradiation (TBI) with mice which retained Bax in Tie2+ cells. Mice bearing deletion of Bak and Bax in Tie2+ cells demonstrated protection of BM HSCs, preserved BM vasculature, and 100% survival following lethal dose TBI. In contrast, mice that retained Bax expression in Tie2+ cells demonstrated depletion of BM HSCs, disrupted BM vasculature, and 10% survival post-TBI. In a complementary study, VEcadherinBak/BaxFl/− mice, which lack Bak and Bax in VEcadherin+ ECs, also demonstrated increased recovery of BM stem/progenitor cells following TBI compared to mice which retained Bax in VEcadherin+ ECs. Importantly, chimeric mice that lacked Bak and Bax in HSCs but retained Bak and Bax in BM ECs displayed significantly decreased HSC content and survival following TBI compared to mice lacking Bak and Bax in both HSCs and BM ECs. These data suggest that the hematopoietic response to ionizing radiation is dependent upon HSC-autonomous responses but is regulated by BM EC-mediated mechanisms. Therefore, BM ECs may be therapeutically targeted as a means to augment hematopoietic reconstitution following myelosuppression.
Electrical properties of hydrocarbon‐derived electrolytes for supercapacitors
Authors: Noorden Zulkarnain A. , Sugawara Sougoro , Matsumoto Satoshi 
Published: November 2012

AbstractThe paper evaluates the essential electrical properties—capacitance and equivalent series resistance (ESR)—of hydrocarbon‐derived electrolytes for supercapacitors using galvanostatic, constant resistance charge–discharge, cyclic voltammetry, and AC impedance measurements. The electrolytes were systematically prepared from six hydrocarbon‐derived compounds, which have different carbon chains and functional groups, with the treatment of concentrated sulfuric acid (H2SO4). Both electrical properties seem to be in conformity throughout these measurements, with the longer main carbon chain compounds giving higher capacitance and lower ESR values. Comparison between these electrolytes and the typically used aqueous solutions in supercapacitor research—1M H2SO4, 5M potassium hydroxide, and 5M sodium hydroxide—showed that the produced electrolytes give the highest capacitance value up to 30 times more than that of typical aqueous solutions. It is believed that with further treatment these hydrocarbon‐derived electrolytes can potentially be used in supercapacitors as better performing electrolytes than aqueous solutions and at a relatively low cost compared to organic solutions. IEEJ Trans 2011 DOI: 10.1002/tee.21802