bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allometric relations between fluvial impulse and morphometric parameters of floodplain lakes
Authors: Ferencz Beata , Dawidek Jarosław 
Published: July 2015

AbstractThe general role of river water input in shaping the basic morphometric parameters of floodplain lakes has been previously investigated. However, the process has not been quantitatively described in detail. This study is the first attempt in the literature to determine the allometric relation between fluvial impulse, expressed as Fluvial Connectivity Quotient, and morphometric parameters of six floodplain lakes of Bug River valley in the period 1952–2014. This relationship is given by Y = aXb, from which the value of b exponent was analysed to determine the strength of the allometric relation. Extreme values of allometric compounds during the time period under study ranged from 5.99 to −4.91. Volume was the morphometric parameter showing the highest variability in all the lakes. General similarity in allometric relations was observed in the lakes under study. During analysis, no long‐term trends were observed in the relationship between the Fluvial Connectivity Quotient and morphometric parameters. The results obtained show that fluvial impulse was the factor determining the variability of morphometric parameters of the lakes. Direct catchments topography of lake has periodically (during limnophase periods) played a significant role in shaping the morphometry of floodplain lakes. The most stable allometric relations occurred in a confluent lake, with a low limnological effective rise value and consequently, relatively long potamophase periods.Copyright © 2015 John Wiley & Sons, Ltd.
Experimental and Theoretical Charge Density Study on Di‐2‐pyrazylamine (Hdpza) Molecule in Crystal
Authors: Wu Lai‐Chin , Lee Gene‐Hsiang 
Published: July 2015

AbstractElectron density distribution of Di‐2‐pyrazylamine (Hdpza) is studied both by single‐crystal X‐ray diffraction method at 100K and theoretical calculation. Structural determination reveals that Hdpza molecules crystalize in a syn‐anti conformation with an intramolecular CHN hydrogen bond between two pyrazine rings and then gather together via two intermolecular NHN and CHN hydrogen interaction and ππ stacking interaction between pyrazine rings. Charge density analysis is made in terms of deformation density (Δπ), Laplacian distribution and topological analysis of total electron density based on multipole model and theoretical calculation. The agreement between experiment and theory is good. The topological properties at bond critical points of CC and CN bonds reveal a covalent bond character, and those of intermolecular interactions, such as hydrogen bonds and ππ stacking interactions, reveal a closed‐shell interaction. The potential energy curve of Hdpza molecule shows that the syn‐anti conformation is the most stable one (global minima) than the other two of syn‐syn and anti‐anti conformations.
Anticandida Agents from a Tanzanian Plant Albizia anthelmintica
Authors: Runyoro Deborah K. B. , Joseph Cosam C. , Ngassapa Olipa D. , Darokar Mahendra P. , Srivastava Santosh K. , Matee Mecky I. N. , Wright Colin W. 
Published: July 2015

AbstractCandidiasis is one of the most frequent opportunistic infections in individuals with severe immunosupression and further development of resistance against the available antifungal drugs has created an alarming situation. This requires intensive drug discovery to develop new, more effective, affordable and accessible antifungal agents possessing novel modes of action. Albizia anthelmintica, which is ethno medically used to treat vaginal candidiasis in the Morogoro and coastal regions of Tanzania, on activity guided fractionation and subsequent purification resulted in the isolation and characterization of an isomer of methyl cyclitol (1) and six echinocystic acid saponins (2–7). Saponins 6 and 7 are new and being reported for the first time from nature. Among all the isolated compounds, 3‐O‐[α‐L‐arabinopyranosyl (1→2)][α‐L‐arabinopyranosyl (1→6)]‐2‐acetamido‐2‐deoxy‐β‐D‐glucopyranosyl echinocystic acid (4), 3‐O‐[α‐L‐arabinopyranosyl (1→2)] [α‐L‐arabinopyranosyl (1→6)]‐2‐amino‐2‐deoxy‐β‐D‐glucopyranosyl echinocystic acid (6) and 3‐O‐[β‐D‐glucopyranosyl (1→3)] [α‐L‐arabinopyranosyl (1→2)] [α‐L‐arabinopyranosyl (1→6)]‐2‐amino‐2‐deoxy‐β‐D‐glucopyranosyl echinocystic acid (7) and their combinations were active against the various strains of C. albicans with MICs ranging from 12.5 to 125 μg/ml.
Implantable microelectromechanical sensors for diagnostic monitoring and post‐surgical prediction of bone fracture healing
Authors: McGilvray Kirk C. , Unal Emre , Troyer Kevin L. , Santoni Brandon G. , Palmer Ross H. , Easley Jeremiah T. , Demir Hilmi Volkan , Puttlitz Christian M. 
Published: July 2015

ABSTRACTThe relationship between modern clinical diagnostic data, such as from radiographs or computed tomography, and the temporal biomechanical integrity of bone fracture healing has not been well‐established. A diagnostic tool that could quantitatively describe the biomechanical stability of the fracture site in order to predict the course of healing would represent a paradigm shift in the way fracture healing is evaluated. This paper describes the development and evaluation of a wireless, biocompatible, implantable, microelectromechanical system (bioMEMS) sensor, and its implementation in a large animal (ovine) model, that utilized both normal and delayed healing variants. The in vivo data indicated that the bioMEMS sensor was capable of detecting statistically significant differences (p‐value <0.04) between the two fracture healing groups as early as 21 days post‐fracture. In addition, post‐sacrifice micro‐computed tomography, and histology data demonstrated that the two model variants represented significantly different fracture healing outcomes, providing explicit supporting evidence that the sensor has the ability to predict differential healing cascades. These data verify that the bioMEMS sensor can be used as a diagnostic tool for detecting the in vivo course of fracture healing in the acute post‐treatment period. © 2015 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 33:1439–1446, 2015.
Effect of supplementation of cows diet with linseed and fish oil and different variants of β‐lactoglobulin on fatty acid composition and antioxidant capacity of milk
Authors: Puppel Kamila , Kuczyńska Beata , Nałęcz‐Tarwacka Teresa , Gołębiewski Marcin , Sakowski Tomasz , Kapusta Aleksandra , Budziński Arkadiusz , Balcerak Marek 
Published: July 2015

AbstractBACKGROUNDThe aim of this study was to determine the influence of polymorphic variants of β‐lactoglobulin in cows supplemented with linseed and fish oil on the fatty acid composition and antioxidant capacity of milk. From the herd of 320 Polish Holstein Friesian cows three groups of cows were selected according to the variants of β‐LG (β‐LGAA, β‐LGBB, β‐LGAB). During the first 7 days (the initial period) all the cows were fed the same total mixed ration (TMR) diet. From day 8 to 28,150 g fish oil and 250 g linseed (FOL) was added to the TMR diet of each cow.RESULTSThe results showed that the diet supplemented with FOL was effective in reducing atherogenic and thrombogenic indices. Introducing supplementation improved the antioxidant capacity: higher concentration of C18:2cis‐9 trans‐11, C20:5 n‐3, C22:6 n‐3, bioactive whey proteins and vitamin soluble in fat has been recorded. The results showed that β‐LGAA was associated with lower levels of atherogenic and thrombogenic indices and higher concentration of C22:5 n‐6, phospholipids and β‐carotene. β‐LGBB favours a higher content of C18:1trans‐11, C18:2cis‐9 trans‐11 and lactoferrin. β‐LGAB was associated with higher concentrations of C20:5 n‐3, Lysozyme, α‐retinol, α‐tocopherol and total antioxidant status.CONCLUSIONModification of the diet of cows with fish oil and linseed significantly influenced fatty acid composition and antioxidant properties of milk. The effect of β‐LG phenotype on the fatty acid composition and antioxidant capacity of milk is variable, which could partly be the result of a β‐LG phenotype × diet interaction. © 2015 Society of Chemical Industry
Varicella‐zoster virus–specific cell‐mediated immunity in Ramsay Hunt syndrome
Authors: Haginomori Shin‐Ichi , Ichihara Takahiro , Mori Atsuko , Kanazawa Atsuko , Kawata Ryo , Tang Huamin , Mori Yasuko 
Published: July 2015

Objectives/HypothesisThe etiology of Ramsay Hunt syndrome (Hunt syndrome) is reactivation of latent varicella‐zoster virus (VZV) in the geniculate ganglion of the facial nerve, leading to neuritis. Although the mechanism of the VZV reactivation is unclear, one possibility is that the reactivation involves a low level of VZV‐specific cell‐mediated immunity (CMI). The aim of this study was to clarify the characteristics of the VZV‐specific CMI in Hunt syndrome compared to that in Bell's palsy, and to obtain clues to its role in the development of Hunt syndrome.Study DesignProspective study.MethodsWe determined the median spot numbers and examined VZV‐specific CMI in patients with Hunt syndrome and with Bell's palsy using interferon‐γ enzyme‐linked immunospot (ELISPOT) assays. We analyzed the relationship between the value of VZV‐specific CMI and days from disease onset.ResultsThe median spot number in Hunt syndrome (87.3 spot‐forming cells [SFCs]/4 × 105 peripheral blood mononuclear cells [PBMCs]) was higher than that in Bell's palsy (62.3 SFCs/4 × 105 PBMCs). Hunt syndrome showed a strong relationship between the ELISPOT count and days from onset (r = 0.65). Within the first 5 days from onset, no ELISPOT counts higher than 80 SFCs/4 × 105 PBMCs were observed. On the other hand, no correlation was observed between the ELISPOT count and days from onset in patients with Bell's palsy (r = −0.19).ConclusionsThese results suggest that VZV‐specific CMI in Hunt syndrome is low at disease onset and increases rapidly thereafter. Consequently, reduced VZV‐specific CMI may play an important role in the reactivation of VZV in the facial nerve, leading to Hunt syndrome.Level of Evidence4 Laryngoscope, 126:E35–E39, 2016
Photodynamic therapy for premalignant lesions of the vulva and vagina: A long‐term follow‐up study
Authors: Choi Min Chul , Kim Mi Sun , Lee Gee Hoon , Jung Sang Geun , Park Hyun , Joo Won Duk , Lee Chan , Lee Je Ho , Hwang Yoon Young , Kim Seung Jo 
Published: July 2015

Background and ObjectiveWe aimed to evaluate responses to photodynamic therapy (PDT) and its long‐term efficacy in preserving normal anatomy and function in women with premalignant lesions of the lower genital tract.Study Design/Materials and MethodsFifteen patients received PDT for vulvar intraepithelial neoplasia (VIN), vaginal intraepithelial neoplasia (VAIN), or vulvar Paget's disease between January 2003 and December 2013. Patients underwent colposcopy and/or vulvoscopy for assessment of lesions. Surface photoillumination with a 630‐nm red laser light was applied to the lesions 48 hours after intravenous injection of 2 mg/kg photosensitizer (PSZ; Photogem®). The light dose to the lesions was 150 J/cm2.ResultsThe median age of the 15 patients (VIN II: 3, VIN III: 4, VAIN II: 2, VAIN III: 3, Paget's disease: 3) was 42.3 years. The complete response (CR) rate was 80% (12/15) at the 3‐month follow‐up and 71.4% (10/14) at the 1‐year follow‐up. There were two cases of persistent disease at the 3‐month follow‐up. One patient with persistent disease underwent partial vulvectomy three times for repetitive recurrence, and the other received secondary PDT with topical 5‐aminolevulinic acid (5‐ALA) and subsequently showed no evidence of disease (NED). Another patient achieved 90% remission through a combination of additional alternative treatments after showing partial response (PR). In two cases of CR, recurrence was observed at the 1‐year follow‐up. Regarding adverse events, photosensitivity reactions such as facial edema and urticaria occurred in 13.3% (2/15) and perineal pain occurred in one patient.ConclusionsPDT may be an effective alternative treatment for premalignant lesions of the female lower genital tract to preserve normal anatomy and sexual function without therapeutic impairment. Lasers Surg. Med. 47:566–570, 2015. © 2015 Wiley Periodicals, Inc.
Blue 470 nm light suppresses the growth of Salmonella enterica and methicillin‐resistant Staphylococcus aureus (MRSA) in vitro
Authors: Bumah Violet V. , Masson‐Meyers Daniela S. , Enwemeka Chukuka S. 
Published: July 2015

Background and ObjectiveEmerging evidence suggests that blue light can photo‐inactivate some bacteria of clinical importance. Consequently, we tested the hypothesis that 470 nm light can suppress growth of two recalcitrant bacteria, MRSA and Salmonella.Materials and MethodsWe plated 5 × 106 and 7 × 106 CFU/ml USA300 strain of MRSA and 1 × 106 CFU/ml of Salmonella enterica serovars Typhimurium and Heidelberg. Plates were irradiated with 55, 110, 165 and 220 J/cm2 of blue light, incubated at 37°C for 24 hours and colony counts determined.ResultsCompared with controls, blue light irradiation produced a significant dose‐dependent reduction in the number of colonies formed by each bacterial strain (P < 0.001). Irradiation of 5 × 106 and 7 × 106 CFU/ml MRSA with 55 J/cm2 produced 92% (4.6 × 106 CFU/ml) and 86% (6 × 106 CFU/ml) inactivation respectively, while 110 and 220 J/cm2 suppressed each MRSA density 100%. Irradiation of Salmonella Typhimurium with 55 and 110 J/cm2 suppressed bacterial growth 31% (3.1 × 105 CFU/ml) and 93% (9.3 × 105 CFU/ml) respectively; while Salmonella Heidelberg was inhibited 11% (1.1 × 105 CFU/ml) and 84% (8.4 × 105 CFU/ml) respectively by the two fluences. Complete inactivation of each Salmonella strain was achieved using 165 or 220 J/cm2.ConclusionThe observed inhibition of Gram‐positive (MRSA) and Gram‐negative (Salmonella) bacteria suggests the versatility of blue light in bacteria eradication, making it a viable intervention strategy for decontamination of food and environments that harbor such bacteria. Lasers Surg. Med. 47:595–601, 2015. © 2015 Wiley Periodicals, Inc.
Parkinson's Disease, Religion, and Spirituality
Authors: Redfern Clare , Coles Alasdair 
Published: July 2015

AbstractBackgroundThere has been increasing interest in recent decades in the interactions between disease, religious faith, and spirituality. An issue specific to neurological disorders is to what extent religiousness and spirituality depend upon the integrity of neuronal pathways.MethodsWe review recent research that investigates the effects of Parkinson's disease (PD) on religious faith and spirituality. Few studies have addressed this issue, but these few illustrate contrasting methodological approaches that yield different conclusions.FindingsOn the one hand, case‐control studies have reported an apparent decrease in both religious practice and beliefs in PD with some influence of laterality of disease onset. In contrast, qualitative studies investigating religious and general coping in PD emphasize that religious faith remains important to patients.ConclusionsMethodological pitfalls are found in both approaches. We conclude that there is little evidence to support claims of reduced spirituality and religious faith in PD. We recommend approaches to future studies that could enable a more nuanced understanding of spiritual and religious changes that might occur in PD.
Sustained Effect of Botulinum Neurotoxin in Myoclonus Owing to Epilepsia Partialis Continua
Authors: Bedarf Janis Rebecca , Marek Milena , Bien Christian G. , Elger Christian E. , Paus Sebastian 
Published: July 2015

AbstractBackgroundEpilepsia partialis continua (EPC) is defined as continuous myoclonic jerking of a body part of cortical origin and often resembles a movement disorder. Unfortunately, anti‐epileptic therapy is frequently ineffective. Currently, the effect of botulinum neurotoxin (BoNT) therapy inEPCis controversial.MethodsWe analyzed case histories, treatment protocols, and video documentation of 5 patients withEPC, who received BoNTtherapy in our movement disorders unit. The Unified Myoclonus Rating Scale was used to document treatment effects.ResultsIn all patients, BoNTtreatment significantly reduced severity and frequency of myoclonus and led to pronounced improvement of speech and arm utilization, regardless of etiology or duration ofEPC.ConclusionBoNTrepresents a safe, effective treatment inEPC, offering long‐term abatement of myoclonus. The substantial functional profit may hint at mechanisms beyond local impairment of neuromuscular transmission, such as modulation of maladaptive cortical plasticity, as observed in dystonia and poststroke spasticity.
The laparoscopic right gastroepiploic lymph node flap transfer for upper and lower limb lymphedema: Technique and outcomes
Authors: Ciudad Pedro , Maruccia Michele , Socas Juan , Lee Ming‐Hsien , Chung Kuo‐Piao , Constantinescu Thomas , Kiranantawat Kidakorn , Nicoli Fabio , Sapountzis Stamatis , Yeo Matthew Sze‐Wei , Chen Hung‐Chi 
Published: July 2015

BackgroundLymph node flap transfer popularity for treatment of extremity lymphedema is increasing quickly. Multiple flap donor sites were described in search of the optimal one. We describe the technique and outcomes of a laparoscopically harvested right gastroepiploic lymph node flap for treatment of extremity lymphedema.MethodsFrom January 2012 to January 2013, 10 consecutive female patients, average age 54.8 years, with International Society of Lymphology stage II–III extremity lymphedema refractory to conservative management were included. Five patients had upper limb breast cancer‐related lymphedema and five patients had lower limb pelvic cancer‐related lymphedema. All patients underwent laparoscopic harvest of the right gastroepiploic lymph node flap, transferred to the wrist and ankle as recipient sites. Flaps were covered with a small skin graft taken from the thigh. Perioperative assessment included physical exam, photography, circumference measurements, CT scans, lymphoscintigraphy, and Lymphedema Quality of Life (LYMQOL) questionnaire. Clinical and CT evaluation of donor‐site morbidity were performed.ResultsThe flap survival rate was 100%, with a mean harvest time of 32 minutes and total operating time of 164 minutes. One case required regrafting for skin graft loss. The mean limb reduction rate was 39.5% at a mean follow‐up of 14.7 months. Perioperative lymphoscintigraphy demonstrated transferred lymph node viability and lymphatic transport improvement. LYMQOL showed a 2.6‐fold quality‐of‐life improvement (P < 0.01). No donor‐site morbidity was encountered.ConclusionsThe use of the laparoscopically harvested right gastroepiploic lymph node flap may be a safe technique that improves limb measurements and quality of life in extremity lymphedema patients. © 2015 Wiley Periodicals, Inc. Microsurgery 37:197–205, 2017.
Changes in mental health outcomes with the intensive in‐home child and adolescent psychiatric service: a multi‐informant, latent consensus approach
Authors: Barbot Baptiste , Bick Johanna , Bentley Mary Jane , Balestracci Kathleen M.B. , Woolston Joseph L. , Adnopoz Jean A. , Grigorenko Elena L. 
Published: July 2015

AbstractThis study investigates the Intensive In‐home Child and Adolescent Psychiatric Service (IICAPS), a large‐scale home‐based intervention that collaboratively engages the family, school, and various other service providers (e.g. health practitioners or judicial systems) to prevent the hospitalization, institutionalization or out‐of‐home placement of children and adolescents with serious emotional disturbance. Multi‐informant data (youth, parents and clinician) on the level of youth problem severity and functioning was gathered from 7169 youth and their families served by the IICAPS network, pre‐ and post‐intervention. A newly developed “Multi‐informant Latent Consensus” (MILC) approach was employed to measure mental health “baseline levels” and change, within a Structural Equation Modeling framework. The MILC approach demonstrated promise integrating information from multiple informants involved in the therapeutic process to yield a more accurate and systemic view of a child's level of functioning and problem severity than each report taken individually. Results indicated that the IICAPS family and community based intervention model led to a reduction of problem severity and improved functioning in children and adolescents with severe emotional disturbance. Copyright © 2015 John Wiley & Sons, Ltd.
The Rho‐GTPase effector ROCK regulates meiotic maturation of the bovine oocyte via myosin light chain phosphorylation and cofilin phosphorylation
Authors: Lee So‐Rim , Xu Yong‐Nan , Jo Yu‐Jin , Namgoong Suk , Kim Nam‐Hyung 
Published: July 2015

SUMMARYOocyte meiosis involves a unique asymmetric division involving spindle movement from the central cytoplasm to the cortex, followed by polar body extrusion. ROCK is a Rho‐GTPase effector involved in various cellular functions in somatic cells as well as oocyte meiosis. ROCK was previously shown to promote actin organization by phosphorylating several downstream targets, including LIM domain kinase (LIMK), phosphorylated cofilin (p‐cofilin), and myosin light chain (MLC). In this study, we investigated the roles of ROCK and MLC during bovine oocyte meiosis. We found that ROCK was localized around the nucleus at the oocyte's germinal‐vesicle (GV) stage, but spreads to the rest of the cytoplasm in later developmental stages. On the other hand, phosphorylated MLC (p‐MLC) localized at the cortex, and its abundance decreased by the metaphase‐II stage. Disrupting ROCK activity, via RNAi or the chemical inhibitor Y‐27632, blocked both cell cycle progression and polar body extrusion. ROCK inhibition also resulted in decreased cortical actin, p‐cofilin, and p‐MLC levels. Similar to the phenotype associated with inhibition of ROCK activity, inhibition of MLC kinase by the chemical inhibitor ML‐7 caused defects in polar body extrusion. Collectively, our results suggest that the ROCK/MLC/actomyosin as well as ROCK/LIMK/cofilin pathways regulate meiotic spindle migration and cytokinesis during bovine oocyte maturation. Mol. Reprod. Dev. 82: 849–858, 2015. © 2015 Wiley Periodicals, Inc.
Factors contributing to delay in diagnosis of Guillain‐Barré syndrome and impact on clinical outcome
Authors: Dubey Divyanshu , Kapotic Marissa , Freeman Matthew , Sawhney Anshudha , Rojas Julio C. , Warnack Worthy , Vernino Steven 
Published: July 2015

ABSTRACTIntroductionHeterogeneity of presenting symptoms makes the initial clinical diagnosis of Guillain‐Barré syndrome (GBS) challenging.MethodsObservational retrospective study from 2 teaching hospitals (Parkland Memorial Hospital and University of Texas Southwestern University Hospital) between 2008 and 2013.ResultsSixty‐nine GBS patients were identified. GBS was suspected on initial emergency department visit in only 49%. During first hospital encounter, 58% were evaluated by a neurologist. Neuropathic pain and presence of intact deep tendon reflexes were associated with delayed GBS diagnosis (P < 0.05). There was significantly better clinical outcome among patients who were evaluated by a neurologist during the initial visit (P < 0.005). Among these patients there was also significant difference in discharge destination; 71.2% of patients evaluated by a neurologist were discharged home (P < 0.01). Patients in whom GBS was not suspected at the time of initial Neurology evaluation were more likely to require intubation and to have residual weakness at the time of discharge (P < 0.05).ConclusionsAtypical clinical signs and symptoms may lead to delayed diagnosis of GBS. Early neurological evaluation is associated with improved clinical diagnosis and discharge disposition. Muscle Nerve 53: 384–387, 2016