bluuebunny/crossref_metadata_2025_split huggingface dataset:
Propuesta para el diseño de rutas turísticas culturales “El caso del sur del estado de Jalisco, México”
Authors: Ochoa Pablo Noé Arredondo , Vega Carlos Hernández , Tolentino Tania Delfina Mendoza 
Published: August 2014

El presente trabajo es uno de los resultados de una tesis de especialidad en turismo, donde se presenta una propuesta para la valoración del patrimonio cultural con el fin de crear rutas de turismo cultural, caso de la región sur del estado de Jalisco, México. Se analizan los factores de accesibilidad y disponibilidad para poder hacer de un patrimonio un destino turístico, así como, los factores que se deben tomar en cuenta para decidir los atractivos a incluir en la ruta, sus características, los servicios que coadyuvan en la elaboración de un producto turístico competitivo (hospedaje, alimentación, transporte y servicios complementarios). También se incluye sugerencias para la capacitación a los prestadores de servicios turísticos (PST), la señalización de atractivos, distancias y sitios, a su vez la comercialización de la ruta como producto turístico, es decir, las estrategias que se recomienda utilizar para posicionar la ruta de turismo cultural, quienes son los evaluadores de la ruta turística y por ultimo dónde empezar y dónde concluir la ruta de acuerdo a la disponibilidad de tiempo y la disponibilidad económica del turista.
Cenários para o Setor Turístico no Estado do Ceará (Brasil) para o período de 2013 a 2023
Authors: Carvalho Camila Cruz de , Albuquerque Joyce Silva , Sales Raissa Karen Leitinho , Cardoso Márcia Lopes , Valdevino Rosângela Queiroz Souza , Forte Sérgio Henrique Arruda Cavalcante 
Published: August 2014

Esta pesquisa tem como objetivo geral elaborar cenários mais prováveis para o turismo no estado do Ceará (Brasil), considerando o período de 2013 a 2023, e tem como objetivo específico propor estratégias públicas e privadas para os cenários prospectados. A metodologia utilizada é de cunho descritivo, qualitativo, utilizando-se de material secundário, além de consulta a expertises e apoio da matriz MICMAC. O resultado do trabalho foi a elaboração de dois cenários, um realista e outro pessimista, com a proposição de políticas públicas para cada um deles. O cenário realista projetaque o mix de serviços oferecidos pelas empresas e o Estado será ampliado, no que se refere a atrações turísticas cearenses. Para o cenário pessimista, considera-se que haverá um encolhimento do investimento em atrações turísticas no Ceará, com redução do mix dos serviços oferecidos pelas empresas e menor valorização da capacitação da mão de obra local.
SOX9 as a Predictor for Neurogenesis Potentiality of Amniotic Fluid Stem Cells
Authors: Wei Pei-Cih , Chao Angel , Peng Hsiu-Huei , Chao An-Shine , Chang Yao-Lung , Chang Shuenn-Dyh , Wang Hsin-Shih , Chang Yu-Jen , Tsai Ming-Song , Sieber Martin , Chen Hua-Chien , Chen Shu-Jen , Lee Yun-Shien , Hwang Shiaw-Min , Wang Tzu-Hao 
Published: August 2014

Abstract
Preclinical studies of amniotic fluid-derived cell therapy have been successful in the research of neurodegenerative diseases, peripheral nerve injury, spinal cord injury, and brain ischemia. Transplantation of human amniotic fluid stem cells (AFSCs) into rat brain ventricles has shown improvement in symptoms of Parkinson's disease and also highlighted the minimal immune rejection risk of AFSCs, even between species. Although AFSCs appeared to be a promising resource for cell-based regenerative therapy, AFSCs contain a heterogeneous pool of distinct cell types, rendering each preparation of AFSCs unique. Identification of predictive markers for neuron-prone AFSCs is necessary before such stem cell-based therapeutics can become a reality. In an attempt to identify markers of AFSCs to predict their ability for neurogenesis, we performed a two-phase study. In the discovery phase of 23 AFSCs, we tested ZNF521/Zfp521, OCT6, SOX1, SOX2, SOX3, and SOX9 as predictive markers of AFSCs for neural differentiation. In the validation phase, the efficacy of these predictive markers was tested in independent sets of 18 AFSCs and 14 dental pulp stem cells (DPSCs). We found that high expression of SOX9 in AFSCs is associated with good neurogenetic ability, and these positive correlations were confirmed in independent sets of AFSCs and DPSCs. Furthermore, knockdown of SOX9 in AFSCs inhibited their neuronal differentiation. In conclusion, the discovery of SOX9 as a predictive marker for neuron-prone AFSCs could expedite the selection of useful clones for regenerative medicine, in particular, in neurological diseases and injuries.
Identification of a new dust‐stratocumulus indirect effect over the tropical North Atlantic
Authors: Doherty Owen M. , Evan Amato T. 
Published: August 2014

AbstractOver the tropical North Atlantic, during boreal summer, both stratocumulus clouds and mineral aerosols are ubiquitous. We find that low cloud fraction increases in response to high mineral aerosols loadings by 3% to 10% over much of the tropical North Atlantic, in International Satellite Cloud Climatology Project (ISSCP) and Pathfinder Atmosphere Extended (PATMOSx) observations. Using the single‐column mode of the Community Earth System Model (CESM), we estimate that this indirect effect nets a surface cooling of approximately −3 Wm−2 to −12 Wm−2 per unit of dust optical depth (DAOD) increase in mineral aerosols, similar to observed radiative forcings of −4 Wm−2 to −6 Wm−2 per unit of DAOD in ISSCP and PATMOSx. Increases in stratocumulus clouds are linked to increases in atmospheric stability, reductions in boundary layer height, and moistening of the lower atmosphere in response to increased dust load. Mineral dust is shown to behave similarly to other absorbing aerosols in indirectly forcing a response in stratocumulus clouds.
Long‐lived explosive volcanism on Mercury
Authors: Thomas Rebecca J. , Rothery David A. , Conway Susan J. , Anand Mahesh 
Published: August 2014

AbstractThe duration and timing of volcanic activity on Mercury are key indicators of the thermal evolution of the planet and provide a valuable comparative example for other terrestrial bodies. The majority of effusive volcanism on Mercury appears to have occurred early in the planet's geological history (~4.1–3.55 Ga), but there is also evidence for explosive volcanism. Here we present evidence that explosive volcanism occurred from at least 3.9 Ga until less than a billion years ago and so was substantially more long‐lived than large‐scale lava plains formation. This indicates that thermal conditions within Mercury have allowed partial melting of silicates through the majority of its geological history and that the overall duration of volcanism on Mercury is similar to that of the Moon despite the different physical structure, geological history, and composition of the two bodies.
De novo KCNB1 mutations in epileptic encephalopathy
Authors: Torkamani Ali , Bersell Kevin , Jorge Benjamin S. , Bjork Robert L. , Friedman Jennifer R. , Bloss Cinnamon S. , Cohen Julie , Gupta Siddharth , Naidu Sakkubai , Vanoye Carlos G. , George Alfred L. , Kearney Jennifer A. 
Published: August 2014

ObjectiveNumerous studies have demonstrated increased load of de novo copy number variants or single nucleotide variants in individuals with neurodevelopmental disorders, including epileptic encephalopathies, intellectual disability, and autism.MethodsWe searched for de novo mutations in a family quartet with a sporadic case of epileptic encephalopathy with no known etiology to determine the underlying cause using high‐coverage whole exome sequencing (WES) and lower‐coverage whole genome sequencing. Mutations in additional patients were identified by WES. The effect of mutations on protein function was assessed in a heterologous expression system.ResultsWe identified a de novo missense mutation in KCNB1 that encodes the KV2.1 voltage‐gated potassium channel. Functional studies demonstrated a deleterious effect of the mutation on KV2.1 function leading to a loss of ion selectivity and gain of a depolarizing inward cation conductance. Subsequently, we identified 2 additional patients with epileptic encephalopathy and de novo KCNB1 missense mutations that cause a similar pattern of KV2.1 dysfunction.InterpretationOur genetic and functional evidence demonstrate that KCNB1 mutation can result in early onset epileptic encephalopathy. This expands the locus heterogeneity associated with epileptic encephalopathies and suggests that clinical WES may be useful for diagnosis of epileptic encephalopathies of unknown etiology. Ann Neurol 2014;76:529–540
Cerebral localization of impaired phonological retrieval during rhyme judgment
Authors: Pillay Sara B. , Stengel Benjamin C. , Humphries Colin , Book Diane S. , Binder Jeffrey R. 
Published: August 2014

ObjectiveComputation of a prearticulatory phonological representation (phonological access, or phonological retrieval) is an essential process in speech production whose neural localization is not clear. This study combined a specific behavioral measure of phonological access and multivariate voxel‐based lesion‐symptom mapping (VLSM) in a series of left hemisphere stroke patients to identify brain regions critical for this process.MethodsPhonological access was assessed in 40 chronic ischemic stroke patients using a silent rhyming task to avoid confounds with motor planning and articulation deficits. Additional covariates were incorporated in the VLSM analysis to control for orthographic and working memory demands of the rhyming task, and for age, education, and total lesion volume. The resulting t statistic maps were thresholded at voxelwise p < 0.001 and cluster‐corrected at a familywise error of p < 0.05.ResultsPhonological access impairment was correlated with damage to a focal region of cortex and white matter caudal to the posterior sylvian fissure, which included the posterior supramarginal gyrus and adjacent anterior angular gyrus, planum temporale, and posterior superior temporal gyrus. No correlation was observed with Broca's area, insula, or sensorimotor cortex. An additional VLSM showed no correlation between damage in this posterior perisylvian region and spoken word comprehension.InterpretationThis is the first demonstration of a specific lesion correlate for phonological access impairment. Although this posterior perisylvian region overlaps with some versions of the classical Wernicke area, the present results demonstrate its involvement in prearticulatory phonological production rather than speech perception or lexical–semantic processes. Ann Neurol 2014;76:738–746
Self‐Assembly of Molecular Borromean Rings from Bimetallic Coordination Rectangles
Authors: Huang Sheng‐Li , Lin Yue‐Jian , Li Zhen‐Hua , Jin Guo‐Xin 
Published: August 2014

AbstractIn contrast to conventional stepwise synthesis of molecular Borromean rings, a self‐assembly synthetic method which proceeds without the aid of a template has been developed. In the formation of molecular rectangles, by adjustment of the long‐arm length of the rectangles, a series of size‐dependent Borromean‐link frameworks were constructed. Both the shortest length of two arms and the relative proportion of the length of the long arm to that of the short arm play a key role in the formation of Borromean rings. DFT calculations were used to provide theoretical support for the formation of discrete interlocked frameworks.
Hydrogen from Formic Acid through Its Selective Disproportionation over Sodium Germanate—A Non‐Transition‐Metal Catalysis System
Authors: Amos Ruth I. J. , Heinroth Falk , Chan Bun , Zheng Sisi , Haynes Brian S. , Easton Christopher J. , Masters Anthony F. , Radom Leo , Maschmeyer Thomas 
Published: August 2014

AbstractA robust catalyst for the selective dehydrogenation of formic acid to liberate hydrogen gas has been designed computationally, and also successfully demonstrated experimentally. This is the first such catalyst not based on transition metals, and it exhibits very encouraging performance. It represents an important step towards the use of renewable formic acid as a hydrogen‐storage and transport vector in fuel and energy applications.
Hot‐Electron‐Transfer Enhancement for the Efficient Energy Conversion of Visible Light
Authors: Yu Sungju , Kim Yong Hwa , Lee Su Young , Song Hyeon Don , Yi Jongheop 
Published: August 2014

AbstractGreat strides have been made in enhancing solar energy conversion by utilizing plasmonic nanostructures in semiconductors. However, current generation with plasmonic nanostructures is still somewhat inefficient owing to the ultrafast decay of plasmon‐induced hot electrons. It is now shown that the ultrafast decay of hot electrons across Au nanoparticles can be significantly reduced by strong coupling with CdS quantum dots and by a Schottky junction with perovskite SrTiO3 nanoparticles. The designed plasmonic nanostructure with three distinct components enables a hot‐electron‐assisted energy cascade for electron transfer, CdS→Au→SrTiO3, as demonstrated by steady‐state and time‐resolved photoluminescence spectroscopy. Consequently, hot‐electron transfer enabled the efficient production of H2 from water as well as significant electron harvesting under irradiation with visible light of various wavelengths. These findings provide a new approach for overcoming the low efficiency that is typically associated with plasmonic nanostructures.
Self‐Assembly of a Giant Molecular Solomon Link from 30 Subcomponents
Authors: Schouwey Clément , Holstein Julian J. , Scopelliti Rosario , Zhurov Konstantin O. , Nagornov Konstantin O. , Tsybin Yury O. , Smart Oliver S. , Bricogne Gérard , Severin Kay 
Published: August 2014

AbstractThe synthesis of topologically complex structures, such as links and knots, is one of the current challenges in supramolecular chemistry. The so‐called Solomon link consists of two doubly interlocked rings. Despite being a rather simple link from a topological point of view, only few molecular versions of this link have been described so far. Here, we report the quantitative synthesis of a giant molecular Solomon link from 30 subcomponents. The highly charged structure is formed by assembly of 12 cis‐blocked Pt2+ complexes, six Cu+ ions, and 12 rigid N‐donor ligands. Each of the two interlocked rings is composed of six repeating Pt(ligand) units, while the six Cu+ ions connect the two rings. With a molecular weight of nearly 12 kDa and a diameter of 44.2 Å, this complex is the largest non‐DNA‐based Solomon link described so far. Furthermore, it represents a molecular version of a “stick link”.
Olive leaf extract as a crosslinking agent for the preparation of electrospun zein fibers
Authors: Erdogan Ipek , Demir Merve , Bayraktar Oguz 
Published: August 2014

ABSTRACTIncorporating active agents, reinforcing structure by crosslinking, thus changing release properties, can be listed as possible modifications in preparation methods of biopolymer fibers. This study introduces oleuropein, major component of olive leaf extract (OLE), as a natural functional crosslinker for electrospun zein fibers, owing to its antioxidant and antimicrobial properties. Incorporation of OLE causes morphological and structural changes indicated by a decrease in fiber diameter up to 27%, an increase in intensity of NH bending region due to interaction with –OH groups and observation of characteristic oleuropein bands. Extract addition also enhances thermal stability. Zein fibers without OLE is fully degraded at 600°C, whereas 10% of OLE loaded zein fibers is left undegraded. Fifty percent of initial phenolic content loaded into fibers is released which indicate the effect of OLE incorporation as accumulation of oleuropein. OLE‐incorporated fibers immersed in PBS are less fused than pure zein fibers, due to the crosslinking effect. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41338.
Metabolic engineering of Corynebacterium glutamicum for the production of L‐ornithine
Authors: Kim Seo Yun , Lee Joungmin , Lee Sang Yup 
Published: August 2014

ABSTRACTL‐ornithine is a non‐essential amino acid for various industrial applications in food industry. In this study, Corynebacterium glutamicum ATCC 13032 was metabolically engineered for the production of L‐ornithine. First, the proB and argF genes were deleted to block the competitive branch pathway and to block the conversion of L‐ornithine to citrulline, respectively. In addition, the argR gene encoding the regulatory repressor of the L‐arginine operon was also deleted. The resulting strain produced 230 mg/L of L‐ornithine from glucose in flask culture. This base strain was further engineered by the plasmid‐based overexpression of the argCJBD genes from C. glutamicum ATCC 21831, which resulted in the production of 7.19 g/L of L‐ornithine. To enrich the NADPH pool, the carbon flux was redirected towards the pentose phosphate pathway by changing the start codons of the pgi and zwf genes and replacing the native promoter of the tkt operon with the strong sod promoter. Fed‐batch cultivation of this final strain YW06 (pSY223) allowed production of 51.5 g/L of L‐ornithine in 40 h with the overall productivity of 1.29 g/L/h. The results obtained in this study demonstrate the possibility of efficiently producing L‐ornithine by metabolically engineered C. glutamicum. Biotechnol. Bioeng. 2015;112: 416–421. © 2014 Wiley Periodicals, Inc.
Fluorescent protein‐based molecular beacons by zinc finger protein‐guided assembly
Authors: Blackstock Daniel , Sun Qing , Chen Wilfred 
Published: August 2014

ABSTRACTMolecular beacons (MBs) are stem‐loop structured oligonucleotides capable of sensitive and specific nucleic acid detection. However, large‐scale usage of MBs for high throughput applications has been hindered by the many expensive and tedious chemical modifications. In this paper, we reported a new class of fluorescent protein‐based molecular beacons (FP‐MBs) based on zinc finger protein‐guided assembly. The design consisted of a single oligonucleotide which forms a hairpin structure with an extending arm on both ends for the attachment of two different fluorescent proteins upon simple mixing. This new design allows for simplified MB assembly and modifications for a wide range of applications. Biotechnol. Bioeng. 2015;112: 236–241. © 2014 Wiley Periodicals, Inc.
Development and validation of a highly sensitive LC‐MS/MS method for the determination of dexamethasone in nude mice plasma and its application to a pharmacokinetic study
Authors: Yuan Yin , Zhou Xuan , Li Jian , Ye Suofu , Ji Xiwei , Li Liang , Zhou Tianyan , Lu Wei 
Published: August 2014

AbstractIn the current study, a simple, sensitive and rapid analytical method for the determination of dexamethasone was developed and applied to a pharmacokinetic study in nude mice. Using testosterone as an internal standard, a liquid chromatography–tandem mass spectrometry (LC‐MS/MS) approach after one‐step precipitation with acetonitrile was validated and used to determine the concentrations of dexamethasone in nude mice plasma. The method utilized a simple isocratic reverse phase separation over a Dionex C18 column with a mobile phase composed of acetonitrile–water (40:60, v/v). The analyte was detected by a triple quadrupole tandem mass spectrometer via electrospray and multiple reaction monitoring was employed to select both dexamethasone at m/z 393.0/147.1 and testosterone at m/z 289.5/97.3 in the positive ion mode. The calibration curves were linear (r >0.99) ranging from 2.5 to 500 ng/mL with a lower limit of quantitation of 2.5 ng/mL. The relative standard deviation ranged from 1.69 to 9.22% while the relative error ranged from −1.92 to −8.46%. This method was successfully applied to a preclinical pharmacokinetic study of dexamethasone and its pharmacokinetics was characterized by a two‐compartment model with first‐order absorption in female nude mice. Copyright © 2014 John Wiley & Sons, Ltd.
Short Antimicrobial Lipo‐α/γ‐AA Hybrid Peptides
Authors: Li Yaqiong , Smith Christina , Wu Haifan , Teng Peng , Shi Yan , Padhee Shruti , Jones Torey , Nguyen Anh‐My , Cao Chuanhai , Yin Hang , Cai Jianfeng 
Published: August 2014

AbstractThe last two decades have seen the rise of antimicrobial peptides (AMPs) to combat emerging antibiotic resistance. Herein we report the solid‐phase synthesis of short lipidated α/γ‐AA hybrid peptides. This family of lipo‐chimeric peptidomimetics displays potent and broad‐spectrum antimicrobial activity against a range of multi‐drug resistant Gram‐positive and Gram‐negative bacteria. These lipo‐α/γ‐AA hybrid peptides also demonstrate high biological specificity, with no hemolytic activity towards red blood cells. Fluorescence microscopy suggests that these lipo‐α/γ‐AA chimeric peptides can mimic the mode of action of AMPs and kill bacterial pathogens via membrane disintegration. As the composition of these chimeric peptides is simple, therapeutic development could be economically feasible and amenable for a variety of antimicrobial applications.
Mesoporous Carbons Functionalized with Aromatic, Aliphatic, and Cyclic Amines, and their Superior Catalytic Activity
Authors: Chakravarti Rajashree , Kantam M. Lakshmi , Iwai Hideo , Al‐deyab Salem S. , Ariga Katsuhiko , Park Dae‐Hwan , Choy Jin‐Ho , Lakhi Kripal Singh , Vinu Ajayan 
Published: August 2014

AbstractA highly ordered mesoporous carbon has been successfully functionalized with various amino compounds including aliphatic, aromatic, and cyclic diamines via a simple C‐N coupling process. The bonding between the carbon wall structure and the amino groups was confirmed by FT‐IR, Raman, and X‐ray photoelectron spectroscopy. The basicity of the materials can be tuned by simply changing the nature of the diamines on the carbon walls. The catalytic activity of the material as a solid base in the aza‐Michael reaction of amines to activated alkenes was investigated. The functionalized materials were found to be highly active at room temperature and without any solvents, selective and can be recycled several times with consistent activity. The nature of the amine groups on the carbons significantly affects the formation of the β‐amino carbonyl compounds. Among functionalized carbons, carbon with aliphatic diamines showed the highest performance with a high yield of β‐amino carbonyl compounds.
Quinone‐Modified Surfaces for Enhanced Enzyme–Electrode Interactions in Pyrroloquinoline‐Quinone‐Dependent Glucose Dehydrogenase Anodes
Authors: Babanova Sofia , Matanovic Ivana , Atanassov Plamen 
Published: August 2014

AbstractAn approach for enhancing the enzyme–electrode interface reactions with pyrroloquinoline quinone (PQQ)‐dependent glucose dehydrogenase (GDH) is described in this study. Modification of carbonaceous electrodes with ubiquinone or its functional analogues (1,2‐ and 1,4‐benzoquinones) that have the appropriate redox potential to provide a driving force for an electron transfer to occur, along with fast electron‐transfer rate through these molecules, creates an “electron sink” on the electrode surface that can pull electrons from the cofactor, increasing the electron‐transfer rate and generating higher current densities. Several important parameters are experimentally evaluated and/or calculated using density functional theory. Among the quinones investigated, 1,4‐benzoquinone has the greatest influence on the PQQ‐dependent GDH anodes, yielding 5.1‐fold higher current densities on single‐walled, and 3.3‐fold on multi‐walled carbon nanotube papers in comparison to unmodified PQQ‐dependent GDH anodes.