bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo neural regeneration via AAV-NeuroD1 gene delivery to astrocytes in neonatal hypoxic-ischemic brain injury
Authors: Kim Miri , Oh Seokmin , Kim Songyeon , Kim Il-Sun , Kim Joowon , Han Jungho , Ahn Ji Woong , Chung Seungsoo , Jang Jae-Hyung , Shin Jeong Eun , Park Kook In 
Published: July 2024

Abstract
Background
Neonatal hypoxic-ischemic brain injury (HIBI) is a significant contributor to neonatal mortality and long-term neurodevelopmental disability, characterized by massive neuronal loss and reactive astrogliosis. Current therapeutic approaches for neonatal HIBI have been limited to general supportive therapy because of the lack of methods to compensate for irreversible neuronal loss. This study aimed to establish a feasible regenerative therapy for neonatal HIBI utilizing in vivo direct neuronal reprogramming technology.

Methods
Neonatal HIBI was induced in ICR mice at postnatal day 7 by permanent right common carotid artery occlusion and exposure to hypoxia with 8% oxygen and 92% nitrogen for 90 min. Three days after the injury, NeuroD1 was delivered to reactive astrocytes of the injury site using the astrocyte-tropic adeno-associated viral (AAV) vector AAVShH19. AAVShH19 was engineered with the Cre-FLEX system for long-term tracking of infected cells.

Results
AAVShH19-mediated ectopic NeuroD1 expression effectively converted astrocytes into GABAergic neurons, and the converted cells exhibited electrophysiological properties and synaptic transmitters. Additionally, we found that NeuroD1-mediated in vivo direct neuronal reprogramming protected injured host neurons and altered the host environment, i.e., decreased the numbers of activated microglia, reactive astrocytes, and toxic A1-type astrocytes, and decreased the expression of pro-inflammatory factors. Furthermore, NeuroD1-treated mice exhibited significantly improved motor functions.

Conclusions
This study demonstrates that NeuroD1-mediated in vivo direct neuronal reprogramming technology through AAV gene delivery can be a novel regenerative therapy for neonatal HIBI.
A comprehensive review for chronic disease prediction using machine learning algorithms
Authors: Islam Rakibul , Sultana Azrin , Islam Mohammad Rashedul 
Published: July 2024

AbstractThe past few years have seen an emergence of interest in examining the significance of machine learning (ML) in the medical field. Diseases, health emergencies, and medical disorders may now be identified with greater accuracy because of technological advancements and advances in ML. It is essential especially to diagnose individuals with chronic diseases (CD) as early as possible. Our study has focused on analyzing ML’s applicability to predict CD, including cardiovascular disease, diabetes, cancer, liver, and neurological disorders. This study offered a high-level summary of the previous research on ML-based approaches for predicting CD and some instances of their applications. To wrap things up, we compared the results obtained by various studies and the methodologies as well as tools employed by the researchers. The factors or parameters that are responsible for improving the accuracy of the predicting model for different previous works are also identified. For identifying significant features, most of the authors employed a variety of strategies, where least absolute shrinkage and selection (LASSO), minimal-redundancy-maximum-relevance (mRMR), and RELIEF are extensively used methods. It is seen that a wide range of ML approaches, including support vector machine (SVM), random forest (RF), decision tree (DT), naïve Bayes (NB), etc., have been widely used. Also, several deep learning techniques and hybrid models are employed to create CD prediction models, resulting in efficient and reliable clinical decision-making models. For the benefit of the whole healthcare system, we have also offered our suggestions for enhancing the prediction results of CD.
Humor as Hope?
Authors: Skiveren Nicolai 
Published: July 2024

Abstract
This article examines the use of humor in contemporary environmental short films, centering on the alleviating power of humor and its capacity to challenge conventional modes of perception. It argues that humor constitutes an important narrative device in the stories of critical hope that scholars claim are necessary in moving beyond the debilitating registers of apocalyptic rhetoric and crisis discourse. By comparing two short films—the Indian satire Finding Beauty in Garbage, and the American mockumentary The Majestic Plastic Bag—the article examines the affordance of irony, parody, and satire to model alternative and hopeful ways of interacting with contemporary toxic landscapes. The article demonstrates that while genres and devices such as satire, irony, and parody all trouble anthropocentric paradigms of human mastery, they do so in different ways and with different implications. Whereas satire offers an effective vehicle for lamenting the proliferation of waste, the critical mood that defines the genre also restricts its capacity for generating meanings and sensibilities outside conventional environmental discourse. By contrast, parody and irony appear more suited to mobilize such changes, as their playful estrangements model innovative and self-reflexive ways of perceiving waste as a source of beauty, a site of agency, and an object of guilt.
Cognitive Assessment in Grappling Athletes Following Choke versus Nonchoke Submissions
Authors: STELLPFLUG SAMUEL J. , DALRYMPLE KIRSTEN A. , DUMMER MATTHEW F. , SCHINDLER BROC R. , ASHTON SARAH V. , BACHMAN DAVID S. , LEFEVERE ROBERT C. 
Published: July 2024

ABSTRACT

Purpose
Participation in Brazilian jiu-jitsu and mixed martial arts has increased over the last three decades. These sports feature submission attacks, including strangles. These strangles, termed “chokes” in this context, primarily limit blood flow to the brain via compression of neck vasculature. There has been discussion in literature of the possibility of measurable cognitive effects following transient choking episodes. The present study used the King-Devick test (KDT) platform, a tablet-based reaction time and accuracy task designed to measure participants’ number recognition, cognition, and verbal expression. This task requires functional vision, saccadic eye movements, comprehension, and expression.

Methods
Volunteer participants were screened for exclusion (prior brain injury) criteria and survey information before testing. Athletes were tested with the KDT immediately before a Brazilian jiu-jitsu training session, again immediately after succumbing to either a choke (“Choke” arm) or nonchoke (“Non-Choke” arm) submission while sparring, and again after a 10-min rest period following the postsubmission test. Analysis was done on test failures, total test times, and individual difference scores between baseline and subsequent testing.

Results
Sixty-two (32 Choke, 30 Non-Choke) participants were analyzed. There was no significant difference between Choke and Non-Choke in test failures (χ
2(1,62) = 1.25, P = 0.263), total times (t(60) = 0.62, P = 0.540; 95% CI, −3.44 to 6.51), and individual difference scores (t(60) = 0.29, P = 0.776; 95% CI, −2.41 to 3.21).

Conclusions
There were no significant differences between study arms in any of the three analyzed measures. This suggests that cognitive functioning, as measured by the KDT, is not affected by transient choking episodes.
Διερεύνηση της χρήσης βίντεο στο Δημοτικό σχολείο και αποτίμηση της ποιότητας των βίντεο που χρησιμοποιούνται
Authors: Καπανδέλης Γεώργιος , Σοφός Αλιβίζος (Λοΐζος) 
Published: July 2024

Σκοπός της παρούσας εργασίας είναι να διερευνηθούν τόσο ο βαθμός και ο τρόπος χρήσης του βίντεο από τους εκπαιδευτικούς της Πρωτοβάθμιας ως εκπαιδευτικό υλικό όσο και η ποιοτική αποτίμηση των βίντεο που χρησιμοποιούν στη βάση ποιοτικών κριτηρίων. Για την επίτευξη του ακολουθήθηκε μια μεικτή ερευνητική μέθοδος χωρισμένη σε δύο στάδια. Το πρώτο περιλάμβανε έρευνα δημοσκοπικού τύπου, με ερωτηματολόγιο από όπου συλλέχθηκαν δεδομένα σχετικά με τον βαθμό χρήσης, τον τρόπο, τα κριτήρια επιλογής βίντεο αλλά και ηλεκτρονικές διευθύνσεις βίντεο που δήλωσαν οι ερωτηθέντες πως χρησιμοποιούν στην διδασκαλία. Το δεύτερο στάδιο της έρευνας περιλάμβανε την ποσοτικοποιήμενη ανάλυση περιεχομένου αυτών των βίντεο, από δύο ερευνητές με βάση ένα πρωτόκολλο παρατήρησης- ρουμπρίκα που εξετάζει τα ποιοτικά κριτήρια. Συλλέχθηκαν δεδομένα από 150 εκπαιδευτικούς ΠΕ70 & ΠΕ71, και 94 ηλεκτρονικές διευθύνσεις βίντεο, από τα οποία εξετάστηκαν, ως προς τα ποιοτικά χαρακτηριστικά, τελικά τα 42 από δύο ερευνητές. Από τα αποτελέσματα της έρευνας φαίνεται πως οι Έλληνες εκπαιδευτικοί χρησιμοποιούν συχνά βίντεο στην διδασκαλία αναγνωρίζοντας την προστιθέμενη παιδαγωγική αξία του μέσου, αλλά δεν να κάνουν πάντοτε βέλτιστη και ορθή χρήση του. Επιπλέον αν και φαίνεται να επιλέγουν προσεκτικά τα βίντεο που πρόκειται να προβάλουν ώστε να αντιστοιχεί το περιεχόμενο στην ομάδα αναφοράς και τους επιδιωκόμενους στόχους, δεν φαίνεται να έχουν γνώση των ποιοτικών κριτηρίων που προτείνονται από την βιβλιογραφία με αποτέλεσμα τα βίντεο που τελικά χρησιμοποιούν να είναι μέτρια ποιοτικά.
Hardness of Pricing Routes for Two-Stage Stochastic Vehicle Routing Problems with Scenarios
Authors: Ota Matheus J. , Fukasawa Ricardo 
Published: July 2024

On the Difficulty of Pricing Routes for Stochastic Vehicle Routing Problems Many approaches exist for dealing with the uncertainty in the vehicle routing problem with stochastic demands (VRPSD), but the most popular approach models the VRPSD as a two-stage stochastic program where a recourse policy prescribes actions that handle when the realized demands exceed the vehicle capacity. Similarly to other VRP variants, some state-of-the-art algorithms for the VRPSD use set-partitioning formulations that generate variables (routes) via a pricing problem. All of these algorithms, however, have strong assumptions on the probability distribution of customer demands, a simplification that might not be realistic in some applications. In “Hardness of Pricing Routes for Two-Stage Stochastic Vehicle Routing Problems with Scenarios,” Ota and Fukasawa examine the challenges associated with solving the pricing problem of the VRPSD when the customer demands are given by scenarios. They demonstrate that the VRPSD pricing problem is strongly NP-hard for a wide variety of recourse policies and route relaxations. This highlights the difficulty of developing efficient pricing algorithms for the VRPSD with scenario-based demand models.
Inverse Optimization for Routing Problems
Authors: Zattoni Scroccaro Pedro , van Beek Piet , Mohajerin Esfahani Peyman , Atasoy Bilge 
Published: July 2024

We propose a method for learning decision makers’ behavior in routing problems using inverse optimization (IO). The IO framework falls into the supervised learning category and builds on the premise that the target behavior is an optimizer of an unknown cost function. This cost function is to be learned through historical data, and in the context of routing problems, can be interpreted as the routing preferences of the decision makers. In this view, the main contributions of this study are to propose an IO methodology with a hypothesis function, loss function, and stochastic first-order algorithm tailored to routing problems. We further test our IO approach in the Amazon Last Mile Routing Research Challenge, where the goal is to learn models that replicate the routing preferences of human drivers, using thousands of real-world routing examples. Our final IO-learned routing model achieves a score that ranks second compared with the 48 models that qualified for the final round of the challenge. Our examples and results showcase the flexibility and real-world potential of the proposed IO methodology to learn from decision-makers’ decisions in routing problems. History: This paper has been accepted for the Transportation Science Special Issue on TSL Conference 2023. Funding: This work was supported by the European Research Council [TRUST-949796].
A comparative metabolomic analysis reveals the metabolic variations among cartilage of Kashin-Beck disease and osteoarthritis
Authors: Chang Hong , Liu Li , Zhang Qingping , Xu Gangyao , Wang Jianpeng , Chen Ping , Li Cheng , Guo Xianni , Yang Zhengjun , Zhang Feng 
Published: July 2024

AimsThe metabolic variations between the cartilage of osteoarthritis (OA) and Kashin-Beck disease (KBD) remain largely unknown. Our study aimed to address this by conducting a comparative analysis of the metabolic profiles present in the cartilage of KBD and OA.MethodsCartilage samples from patients with KBD (n = 10) and patients with OA (n = 10) were collected during total knee arthroplasty surgery. An untargeted metabolomics approach using liquid chromatography coupled with mass spectrometry (LC-MS) was conducted to investigate the metabolomics profiles of KBD and OA. LC-MS raw data files were converted into mzXML format and then processed by the XCMS, CAMERA, and metaX toolbox implemented with R software. The online Kyoto Encyclopedia of Genes and Genomes (KEGG) database was used to annotate the metabolites by matching the exact molecular mass data of samples with those from the database.ResultsA total of 807 ion features were identified for KBD and OA, including 577 positive (240 for upregulated and 337 for downregulated) and 230 negative (107 for upregulated and 123 for downregulated) ions. After annotation, LC-MS identified significant expressions of ten upregulated and eight downregulated second-level metabolites, and 183 upregulated and 162 downregulated first-level metabolites between KBD and OA. We identified differentially expressed second-level metabolites that are highly associated with cartilage damage, including dimethyl sulfoxide, uric acid, and betaine. These metabolites exist in sulphur metabolism, purine metabolism, and glycine, serine, and threonine metabolism.ConclusionThis comprehensive comparative analysis of metabolism in OA and KBD cartilage provides new evidence of differences in the pathogenetic mechanisms underlying cartilage damage in these two conditions.Cite this article: Bone Joint Res 2024;13(7):362–371.
A sero-epidemiological analysis of Coxiella burnetii infection and its risk factors in livestock from Addis Ababa, Adama, and Modjo abattoirs and pastoral areas of Oromia, Ethiopia
Authors: Milkesa Ashenafi , Rufael Tesfaye , Kinfe Getachew , Belaineh Redeat , Bulbula Abdella , Cho Donghee , Naimuddin Mohammed , Sori Teshale , Dinka Hunduma 
Published: July 2024

Background
Coxiella burnetii is causing infections in both humans and animals, resulting in Q fever and Coxiellosis, respectively. Information on the occurrence of C. burnetii infection is scarce in Ethiopia. This study estimated the sero-prevalence of C. burnetii infection and associated risk factors in four common livestock species from Addis Ababa, Adama, and Modjo abattoirs and pastoral areas of Oromia, Ethiopia.

Results/principal findings
Sera samples were analyzed for the presence of anti-C. burnetii antibodies using an indirect Enzyme Linked Immunosorbent Assay kit. Out of the 4140 serum samples tested, 777 (18.77%; 95% CI: 17.59, 19.99) were found positive for C. burnetii. The sero-prevalence estimate was 27.17% at Addis Ababa abattoir, 19.41% at Adama abattoir, 19.13% at Modjo abattoir and 12.1% in animals tested from pastoral areas. Sera analysis at the animal species level showed that cattle exhibited the lowest sero-prevalence estimate (11.83%; 95% CI, 10.27–13.53%), while the highest was observed in camels (28.39%; 95% CI, 25.16–31.80%). The sero-prevalence estimate was 21.34% (95% CI, 18.86–23.99%) in goats and 20.17% (95% CI, 17.49–23.07%) in sheep. The results of multivariable logistic regression analysis showed that species, age, sex of animals and tick infestation were important risk factors for C. burnetii infection. The odds of infection were 3.22 times higher in camels and almost twice as high in goats and sheep compared to cattle. Adult animals were infected more likely (OR = 3.23) than young ones. Interestingly, a significant difference was observed in the sero-prevalence of infection between animals that were infested with ticks (OR = 16.32) and those which were tick-free.

Conclusion
This study provides valuable insights into the sero-epidemiology of C. burnetii infection in four common livestock species at major abattoirs and pastoral areas of Ethiopia. The findings highlight the need for further studies and implementing surveillance and biosecurity measures to prevent the spread of the disease in both humans and livestock to safeguard the economical and public health aspects.
Bone and joint infections due to melioidosis; diagnostic and management strategies to optimise outcomes
Authors: Dadwal Parvati , Bonner Brady , Fraser David , Loveridge Jeremy , Withey Grant , Puri Arvind , Smith Simon , Hanson Josh 
Published: July 2024

Background
Melioidosis, a life-threatening infection caused by the gram negative bacterium Burkholderia pseudomallei, can involve almost any organ. Bone and joint infections (BJI) are a recognised, but incompletely defined, manifestation of melioidosis that are associated with significant morbidity and mortality in resource-limited settings.

Methodology/principal findings
We identified all individuals with BJI due to B. pseudomallei managed at Cairns Hospital in tropical Australia between January 1998 and June 2023. The patients’ demographics, their clinical findings and their treatment were correlated with their subsequent course.
Of 477 culture-confirmed cases of melioidosis managed at the hospital during the study period, 39 (8%) had confirmed BJI; predisposing risk factors for melioidosis were present in 37/39 (95%). However, in multivariable analysis only diabetes mellitus was independently associated with the presence of BJI (odds ratio (95% confidence interval): 4.04 (1.81–9.00), p = 0.001). BJI was frequently only one component of multi-organ involvement: 29/39 (74%) had infection involving other organs and bacteraemia was present in 31/39 (79%). Of the 39 individuals with BJI, 14 (36%) had osteomyelitis, 8 (20%) had septic arthritis and 17 (44%) had both osteomyelitis and septic arthritis; in 32/39 (83%) the lower limb was involved. Surgery was performed in 30/39 (77%). Readmission after the initial hospitalisation was necessary in 11/39 (28%), 5/39 (13%) had disease recrudescence and 3/39 (8%) had relapse; 4/39 (10%) developed pathological fractures. ICU admission was necessary in 11/39 (28%) but all 11 of these patients survived. Only 1/39 (3%) died, 138 days after admission, due to his significant underlying comorbidity.

Conclusions
The case-fatality rate from melioidosis BJI in Australia’s well-resourced health system is very low. However, recrudescence, relapse and orthopaedic complications are relatively common and emphasise the importance of collaborative multidisciplinary care that includes early surgical review, aggressive source control, prolonged antibiotic therapy, and thorough, extended follow-up.
Humor in parenting: Does it have a role?
Authors: Emery Lucy , Libera Anne , Lehman Erik , Levi Benjamin H. 
Published: July 2024

Background
Despite the widespread use of humor in social interactions and the considerable literature on humor in multiple fields of study, the use of humor in parenting has received very little formal study. The purpose of this pilot study was to gather preliminary data on the use of humor in the raising of children.

Materials and methods
We developed and administered a 10-item survey to measure people’s experiences being raised with humor and their views regarding humor as a parenting tool. Responses were aggregated into Disagree, Indeterminate, and Agree, and analyzed using standard statistical methods.

Results
Respondents (n = 312) predominantly identified as male (63.6%) and white (76.6%) and were (by selection) between the ages of 18–45 years old. The majority of participants reported that they: were raised by people who used humor in their parenting (55.2%); believe humor can be an effective parenting tool (71.8%) and in that capacity has more potential benefit than harm (63.3%); either use (or plan to use) humor in parenting their own children (61.8%); and would value a course on how to utilize humor in parenting (69.7%). Significant correlations were found between the use of humor and both i) the quality of respondents’ relationships with their parents and ii) assessments of how good a job their parents had done.

Conclusions
In this pilot study, respondents of childbearing/rearing age reported positive views about humor as a parenting tool.
Bacteriophages isolated from mouse feces attenuates pneumonia mice caused by Pseudomonas aeruginosa
Authors: Sutnu Nuttawut , Chancharoenthana Wiwat , Kamolratanakul Supitcha , Phuengmaung Pornpimol , Singkham-In Uthaibhorn , Chongrak Chiratchaya , Montathip Sirikan , Wannigama Dhammika Leshan , Chatsuwan Tanittha , Ounjai Puey , Schultz Marcus J. , Leelahavanichkul Asada 
Published: July 2024

Background
Most of the current bacteriophages (phages) are mostly isolated from environments. However, phages isolated from feces might be more specific to the bacteria that are harmful to the host. Meanwhile, some phages from the environment might affect non-pathogenic bacteria for the host.

Methods
Here, bacteriophages isolated from mouse feces were intratracheally (IT) or intravenously (IV) administered in pneumonia mice caused by Pseudomonas aeruginosa at 2 hours post-intratracheal bacterial administration. As such, the mice with phage treatment, using either IT or IV administration, demonstrated less severe pneumonia as indicated by mortality, serum cytokines, bacteremia, bacterial abundance in bronchoalveolar lavage fluid (BALF), and neutrophil extracellular traps (NETs) in lung tissue (immunofluorescence of neutrophil elastase and myeloperoxidase).

Results
Interestingly, the abundance of phages in BALF from the IT and IV injections was similar, supporting a flexible route of phage administration. With the incubation of bacteria with neutrophils, the presence of bacteriophages significantly improved bactericidal activity, but not NETs formation, with the elevated supernatant IL-6 and TNF-α, but not IL-1β. In conclusion, our findings suggest that bacteriophages against Pseudomonas aeruginosa can be discovered from feces of the host.

Conclusions
The phages attenuate pneumonia partly through an enhanced neutrophil bactericidal activity, but not via inducing NETs formation. The isolation of phages from the infected hosts themselves might be practically useful for future treatment. More studies are warranted.