bluuebunny/crossref_metadata_2025_split huggingface dataset:
SpyTag/SpyCatcher Cyclization Confers Resilience to Boiling on a Mesophilic Enzyme
Authors: Schoene Christopher , Fierer Jacob O. , Bennett S. Paul , Howarth Mark 
Published: May 2014

AbstractSpyTag is a peptide that spontaneously forms an amide bond with its protein partner SpyCatcher. SpyTag was fused at the N terminus of β‐lactamase and SpyCatcher at the C terminus so that the partners could react to lock together the termini of the enzyme. The wild‐type enzyme aggregates above 37 °C, with irreversible loss of activity. Cyclized β‐lactamase was soluble even after heating at 100 °C; after cooling, the catalytic activity was restored. SpyTag/SpyCatcher cyclization led to a much larger increase in stability than that achieved through point mutation or alternative approaches to cyclization. Cyclized dihydrofolate reductase was similarly resilient. Analyzing unfolding through calorimetry indicated that cyclization did not increase the unfolding temperature but rather facilitated refolding after thermal stress. SpyTag/SpyCatcher sandwiching represents a simple and efficient route to enzyme cyclization, with potential to greatly enhance the robustness of biocatalysts.
Spontaneous Biomimetic Formation of (±)‐Dictazole B under Irradiation with Artificial Sunlight
Authors: Skiredj Adam , Beniddir Mehdi A. , Joseph Delphine , Leblanc Karine , Bernadat Guillaume , Evanno Laurent , Poupon Erwan 
Published: May 2014

AbstractGuided by biosynthetic considerations, the total synthesis of dictazole B is reported for the first time. Experimental evidence for an easy access to challenging cyclobutane alkaloids of marine origin, which are often postulated to be biosynthetic precursors of more complex structures, is provided.
Preparation and cell morphology of ethylene‐vinyl acetate copolymer (EVA)/wood‐flour foams with low density
Authors: Kim Ji‐Hoo , Kim Gue‐Hyun 
Published: May 2014

ABSTRACTThe main objective of this study is to obtain ethylene‐vinyl acetate copolymer (EVA)/wood‐flour foams with low density (< 0.2 g/cm3) using chemical blowing agent. Stearic acid was used as a compatibilizer to improve not only the compatibility between wood‐flour and EVA but also the compatibility between moisture and EVA in this study. The effects of wood‐flour content on the density and mechanical properties of EVA/wood‐flour foams were studied. Also, the effects of content of stearic acid on the cell morphology of EVA/wood‐flour foams were investigated. The shape of EVA/wood‐flour foams with 20% wood‐flour content becomes more uniform with increasing content of stearic acid. The most stabilized shape of the foams is obtained with 5 wt % stearic acid content. The density of EVA/wood‐flour foams with 20% wood‐flour and 5 wt % stearic acid is 0.11 g/cm3. With increasing content of stearic acid, more gas remains in the EVA matrix and consequently, average cell size and density increase. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 40894.
Novel Au/La‐SrTiO3 microspheres: Superimposed Effect of Gold Nanoparticles and Lanthanum Doping in Photocatalysis
Authors: Wang Guannan , Wang Pei , Luo He‐Kuan , Hor T. S. Andy 
Published: May 2014

AbstractNovel multielement Au/La‐SrTiO3 microspheres were synthesized by a solvothermal method using monodisperse gold and La‐SrTiO3 nanocrystals as building blocks. The porous Au/La‐SrTiO3 microspheres had a large surface area of 94.6 m2 g−1. The stable confined Au nanoparticles demonstrated strong surface plasmon resonance effect, leading to enhanced absorption in a broad UV/Vis/NIR range. Doping of rare‐earth metal La also broadened the absorption band to the visible region. Both the conduction and valence bands of Au/La‐SrTiO3 microspheres thus show favorable potential for proton reduction under visible light. The superimposed effect of Au nanoparticles and La doping in Au/La‐SrTiO3 microspheres led to high photocurrent density in photoelectrochemical water splitting and good photocatalytic activity in photodegradation of rhodamine B. The photocatalytic activities are in the order of the following: Au/La‐SrTiO3 microspheres>Au/SrTiO3 microspheres>La‐SrTiO3 microspheres>SrTiO3 microspheres.
Dual Association Modes of the 2,5,8‐Tris(pentafluorophenyl)phenalenyl Radical
Authors: Uchida Kazuyuki , Hirao Yasukazu , Kurata Hiroyuki , Kubo Takashi , Hatano Sayaka , Inoue Katsuya 
Published: May 2014

AbstractThe 2,5,8‐tris(pentafluorophenyl)phenalenyl radical was obtained by a straightforward synthesis in 11 steps from 2,7‐dibromonaphthalene. This radical crystallized as a σ dimer from a solution in MeCN and as a π‐stack from a melted liquid. The π stack was not confined to dimerization, but extended into a uniform 1D stack with an interplanar distance of 3.503 Å. This unique duality in association mode arose from the thermodynamic stability of the phenalenyl moiety.
Dynamic T‐S Fuzzy Systems Identification Based on Sparse Regularization
Authors: Luo Minnan , Sun Fuchun , Liu Huaping 
Published: May 2014

AbstractFuzzy system identification suffers from rules explosion, i.e., a large number of fuzzy rules are required for fuzzy systems with high dimension input variable. In this paper, a dynamic algorithm is proposed to address T‐S fuzzy system identification with both sparsity and dynamic clustering, named as dynamic sparse fuzzy inference systems (D‐sparseFIS). Due to two different estimation approaches of fuzzy rule consequence parameter, i.e., global estimation and local estimation, D‐sparseFIS.local and D‐sparseFIS.global methods are exploited with local least squares and global least squares estimation based on sparse regularization. Both two dynamic algorithms can guarantee a minimal number of fuzzy rules and nonzero consequence parameters are equipped in T‐S fuzzy system. Finally, some numerical experiments are presented to illustrate the effectiveness of the proposed algorithms.
Distributed Robust H∞ Consensus for Multi‐Agent Systems with Nonlinear Dynamics and Parameter Uncertainties
Authors: Wang Yinqiu , Wu Qinghe 
Published: May 2014

AbstractIn this study, the consensus problem is discussed for a class of multi‐agent systems with unknown Lipschitz nonlinear dynamics, external disturbances, and parameter uncertainties under a fixed directed graph. By some model transformations, the consensus problem becomes a reduced‐order H∞ control problem. Based on the reduced‐order control problem, sufficient conditions to achieve the consensus with the desired H∞ performance are presented. These conditions are to check the solvability of only one linear matrix inequality (LMI) and the dimension of the LMI independent of the number of agents in the network. Moreover, an algorithm is proposed to design the dynamic output feedback controller. Simulation on networked multi‐agents is provided to show the effectiveness of the theoretical results.
A ranking of high‐resolution daily precipitation extreme events for the Iberian Peninsula
Authors: Ramos Alexandre M. , Trigo Ricardo M. , Liberato Margarida L. R. 
Published: May 2014

AbstractA method for ranking daily precipitation events is presented based on the most comprehensive database of daily gridded precipitation dataset available for the Iberian Peninsula spanning from 1950 to 2008. The magnitude of an event is obtained after considering the area affected as well as its intensity in every grid point and taking into account the daily normalized departure from climatology. Different precipitation rankings are presented considering the entire Iberian Peninsula, Portugal and also the six largest river basins in the Iberian Peninsula.
Interactive MOLP explorer—A graphical‐based computational tool for teaching and decision support in multi‐objective linear programming models
Authors: Alves Maria João , Antunes Carlos Henggeler , Clímaco João 
Published: May 2014

ABSTRACTThis article presents an interactive computational tool for teaching and providing decision support in multi‐objective linear programming models. The main aim is to offer students in engineering and economics & management a user‐friendly environment where the main theoretical and methodological concepts of multi‐objective optimization can be apprehended through experimentation. © 2014 Wiley Periodicals, Inc. Comput Appl Eng Educ 23:314–326, 2015; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.21601
Identification and expression of troponin T, a new marker on the surface of cultured tumor endothelial cells by aptamer ligand
Authors: Ara Mst. Naznin , Hyodo Mamoru , Ohga Noritaka , Akiyama Kosuke , Hida Kyoko , Hida Yasuhiro , Shinohara Nobuo , Harashima Hideyoshi 
Published: May 2014

AbstractThe identification of a specific biomarker involves the development of new clinical diagnostic tools, and an in‐depth understanding of the disease at the molecular level. When new blood vessels form in tumor cells, endothelial cell production is induced, a process that plays a key role in disease progression and metastasis to distinct organs for solid tumor types. The present study reports on the identification of a new biomarker on primary cultured mouse tumor endothelial cells (mTECs) using our recently developed high‐affinity DNA aptamer AraHH001 (Kd = 43 nmol/L) assisted proteomics approach. We applied a strategy involving aptamer‐facilitated biomarker discovery. Biotin‐tagged AraHH001 was incubated with lysates of mTECs and the aptamer‐proteins were then conjugated with streptavidin magnetic beads. Finally, the bound proteins were separated by sodiumdodecyl sulfate polyacrylamide gel electrophoresis (SDS‐PAGE) with silver staining. We identified troponin T via matrix assisted laser desorption ionization‐time of flight (MALDI‐TOF) mass spectrometry, the molecular target of aptamer AraHH001, and its presence was confirmed by measuring mRNA, protein levels, western blot, immunostaining, a gel shift assay of AraHH001 with troponin T. We first report here on the discovery of troponin T on mTECs, a promising and interesting diagnostic tool in the development of antiangiogenic therapy techniques the involves the targeting of the tumor vasculature.
The role of laser interstitial thermal therapy in enhancing progression‐free survival of difficult‐to‐access high‐grade gliomas: a multicenter study
Authors: Mohammadi Alireza M. , Hawasli Ammar H. , Rodriguez Analiz , Schroeder Jason L. , Laxton Adrian W. , Elson Paul , Tatter Stephen B. , Barnett Gene H. , Leuthardt Eric C. 
Published: May 2014

AbstractSurgical extent‐of‐resection has been shown to have an impact on high‐grade glioma (HGG) outcomes; however, complete resection is rarely achievable in difficult‐to‐access (DTA) tumors. Controlled thermal damage to the tumor may have the same impact in DTA‐HGGs. We report our multicenter results of laser interstitial thermal therapy (LITT) in DTA‐HGGs. We retrospectively reviewed 34 consecutive DTA‐HGG patients (24 glioblastoma, 10 anaplastic) who underwent LITT at Cleveland Clinic, Washington University, and Wake Forest University (May 2011–December 2012) using the NeuroBlate® System. The extent of thermal damage was determined using thermal damage threshold (TDT) lines: yellow TDT line (43°C for 2 min) and blue TDT line (43°C for 10 min). Volumetric analysis was performed to determine the extent‐of‐coverage of tumor volume by TDT lines. Patient outcomes were evaluated statistically. LITT was delivered as upfront in 19 and delivered as salvage in 16 cases. After 7.2 months of follow‐up, 71% of cases demonstrated progression and 34% died. The median overall survival (OS) for the cohort was not reached; however, the 1‐year estimate of OS was 68 ± 9%. Median progression‐free survival (PFS) was 5.1 months. Thirteen cases who met the following two criteria—(1) <0.05 cm3 tumor volume not covered by the yellow TDT line and (2) <1.5 cm3 additional tumor volume not covered by the blue TDT line—had better PFS than the other 21 cases (9.7 vs. 4.6 months; P = 0.02). LITT can be used effectively for treatment of DTA‐HGGs. More complete coverage of tumor by TDT lines improves PFS which can be translated as the extent of resection concept for surgery.
Rapid Total Synthesis of Cyclic Lipodepsipeptides as a Premise to Investigate their Self‐Assembly and Biological Activity
Authors: De Vleeschouwer Matthias , Sinnaeve Davy , Van den Begin Jos , Coenye Tom , Martins José C. , Madder Annemieke 
Published: May 2014

AbstractA rapid and efficient total synthesis is reported for the cyclic lipodepsipeptide pseudodesmin A. This member of the Pseudomonas viscosin group is active against Gram‐positive bacteria and features self‐assembling properties. A conserved serine residue within the lactone macrocycle is exploited for initial immobilization on 2‐chlorotrityl chloride resin through ether formation with the side‐chain alcohol. Subsequent elongation proceeds through Fmoc solid‐phase peptide synthesis, including automated incorporation of the enantioselectively synthesized (R)‐3‐hydroxydecanoic acid lipid tail. Following esterification to generate the incipient lactone bond, the macrocycle is formed by on‐resin head‐to‐tail macrolactamization and cleaved from the resin to give the desired compound in good purity. The short and efficient synthesis route allows rapid generation of analogues by facile variation of both the peptide and lipid moieties with good control of epimerization while maximizing automation. Synthesis of the pseudodesmin A enantiomer yields identical self‐assembly and biological activity to that observed for the natural compound, showing that activity is not mediated by chiral interactions. A D‐Asn8 analogue developed en route retains self‐assembly, but loses activity. The synthesis strategy should be generally applicable for the rapid generation of analogues from various cyclic lipodepsipeptide groups, allowing an investigation of their self‐assembling properties and structure–activity relationships.
Tuning Thiophene with Phosphorus: Synthesis and Electronic Properties of Benzobisthiaphospholes
Authors: Qiu Yunyan , Worch Joshua C. , Chirdon Danielle N. , Kaur Aman , Maurer Andrew B. , Amsterdam Samuel , Collins Christopher R. , Pintauer Tomislav , Yaron David , Bernhard Stefan , Noonan Kevin J. T. 
Published: May 2014

Abstract1,4‐Dimercapto‐2,5‐diphosphinobenzene and 3,6‐bis(hexyloxy)‐1,4‐dimercapto‐2,5‐diphosphinobenzene were synthesized and combined with various acid chlorides to obtain a series of benzobisthiaphospholes. Electrochemical and photophysical properties of the substituted benzobisthiaphospholes have been evaluated, and the observed reductions are more facile than the related benzothiaphospholes and 2,6‐diphenylbenzobisthiazole. A benzobisthiaphosphole with C6H4‐p‐CN substituents was reduced at E1/2=−1.08 V (vs. saturated calomel electrode (SCE)). X‐ray diffraction data for several of these phosphorus heterocycles has been obtained, and DFT calculations at the B3LYP level have been performed.
Fe or FeNO Catalysis? A Quantum Chemical Investigation of the [Fe(CO)3(NO)]−‐Catalyzed Cloke–Wilson Rearrangement
Authors: Klein Johannes E. M. N. , Knizia Gerald , Miehlich Burkhard , Kästner Johannes , Plietker Bernd 
Published: May 2014

AbstractA quantum chemical investigation of the Bu4N[Fe(CO)3(NO)]‐catalyzed Cloke–Wilson rearrangement of vinyl cyclopropanes is reported. It was found that allylic CC bond activation can proceed through a SN2′ or SN2‐type mechanism. The application of the recently reported intrinsic bond orbital (IBO) method for all structures indicated that one FeN π bond is directly involved. Further analysis showed that during the reaction oxidation occurs at the NO ligand exclusively.
Selective Separation of Ultrafine Particles Using Two‐Liquid Flotation
Authors: Leistner Tom , Müller Martin , Erler Jacqueline V. , Rudolph Martin , Peuker Urs A. 
Published: May 2014

AbstractDie Vergrößerung des effizienten Anwendungsbereiches von Sortierprozessen in den Bereich feinster Partikelsysteme (0,1 – 10 μm) stellt eine erhebliche Herausforderung für die Forschung dar. Ein möglicher Prozessansatz zur Verbesserung des Sortierergebnisses ist die Flüssig/Flüssig‐Flotation. In dieser Studie werden Aussagen bezüglich Anwendbarkeit und Prozessverhalten dieses Ansatzes in Abhängigkeit von ausgewählten Prozessparametern an verschiedenen Modellpartikelsystemen präsentiert.
8‐Benzyltetrahydropyrazino[2,1‐f]purinediones: Water‐Soluble Tricyclic Xanthine Derivatives as Multitarget Drugs for Neurodegenerative Diseases
Authors: Brunschweiger Andreas , Koch Pierre , Schlenk Miriam , Pineda Felipe , Küppers Petra , Hinz Sonja , Köse Meryem , Ullrich Stefan , Hockemeyer Jörg , Wiese Michael , Heer Jag , Müller Christa E. 
Published: May 2014

Abstract8‐Benzyl‐substituted tetrahydropyrazino[2,1‐f]purinediones were designed as tricyclic xanthine derivatives containing a basic nitrogen atom in the tetrahydropyrazine ring to improve water solubility. A library of 69 derivatives was prepared and evaluated in radioligand binding studies at adenosine receptor (AR) subtypes and for their ability to inhibit monoamine oxidases (MAO). Potent dual‐target‐directed A1/A2A adenosine receptor antagonists were identified. Several compounds showed triple‐target inhibition; one of the best compounds was 8‐(2,4‐dichloro‐5‐fluorobenzyl)‐1,3‐dimethyl‐6,7,8,9‐tetrahydropyrazino[2,1‐f]purine‐2,4(1H,3H)‐dione (72) (human AR: Ki A1 217 nM, A2A 233 nM; IC50 MAO‐B: 508 nM). Dichlorinated compound 36 [8‐(3,4‐dichlorobenzyl)‐1,3‐dimethyl‐6,7,8,9‐tetrahydropyrazino[2,1‐f]purine‐2,4(1H,3H)‐dione] was found to be the best triple‐target drug in rat (Ki A1 351 nM, A2A 322 nm; IC50 MAO‐B: 260 nM), and may serve as a useful tool for preclinical proof‐of‐principle studies. Compounds that act at multiple targets relevant for symptomatic as well as disease‐modifying treatment of neurodegenerative diseases are expected to show advantages over single‐target therapeutics.
Mathematical modelling of the pathogenesis of multiple myeloma‐induced bone disease
Authors: Ji Bing , Genever Paul G. , Patton Ronald J. , Fagan Michael J. 
Published: May 2014

SUMMARYMultiple myeloma (MM) is the second most common haematological malignancy and results in destructive bone lesions. The interaction between MM cells and the bone microenvironment plays an important role in the development of the tumour cells and MM‐induced bone disease and forms a ‘vicious cycle’ of tumour development and bone destruction, intensified by suppression of osteoblast activity and promotion of osteoclast activity. In this paper, a mathematical model is proposed to simulate how the interaction between MM cells and the bone microenvironment facilitates the development of the tumour cells and the resultant bone destruction. It includes both the roles of inhibited osteoblast activity and stimulated osteoclast activity. The model is able to mimic the temporal variation of bone cell concentrations and resultant bone volume after the invasion and then removal of the tumour cells and explains why MM‐induced bone lesions rarely heal even after the complete removal of MM cells. The behaviour of the model compares well with published experimental data. The model serves as a first step to understand the development of MM‐induced bone disease and could be applied further to evaluate the current therapies against MM‐induced bone disease and even suggests new potential therapeutic targets. © 2014 The Authors. International Journal for Numerical Methods in Biomedical Engineering published by John Wiley & Sons Ltd
Structural Control of Hierarchically‐Ordered TiO2 Films by Water for Dye‐Sensitized Solar Cells
Authors: Ahn Sung Hoon , Kim Dong Jun , Roh Dong Kyu , Chi Won Seok , Kim Jong Hak 
Published: May 2014

AbstractA facile way of controlling the structure of TiO2 by changing the amount of water to improve the efficiency of dye‐sensitized solar cells (DSSCs) is reported. Hierarchically ordered TiO2 films with high porosity and good interconnectivity are synthesized in a well‐defined morphological confinement arising from a one‐step self‐assembly of preformed TiO2 (pre‐TiO2) nanocrystals and a graft copolymer, namely poly(vinyl chloride)‐g‐poly(oxyethylene methacrylate). The polymer–solvent interactions in solution, which are tuned by the amount of water, are shown to be a decisive factor in determining TiO2 morphology and device performance. Systematic control of wall and pore size is achieved and enables the bifunctionality of excellent light scattering properties and easy electron transport through the film. These properties are characterized by reflectance spectroscopy, incident photon‐to‐electron conversion efficiency, and electrochemical impedance spectroscopy analyses. The TiO2 photoanode that is prepared with a higher water ratio, [pre‐TiO2]:[H2O]=1:0.3, shows a larger surface area, greater light scattering, and better electron transport, which result in a high efficiency (7.7 %) DSSC with a solid polymerized ionic liquid. This efficiency is much greater than that of commercially available TiO2 paste (4.0 %).
A Polyoxovanadate as an Advanced Electrode Material for Supercapacitors
Authors: Chen Han‐Yi , Wee Grace , Al‐Oweini Rami , Friedl Jochen , Tan Kim Soon , Wang Yuxi , Wong Chui Ling , Kortz Ulrich , Stimming Ulrich , Srinivasan Madhavi 
Published: May 2014

AbstractPolyoxovanadate Na6V10O28 is investigated for the first time as electrode material for supercapacitors (SCs). The electrochemical properties of Na6V10O28 electrodes are studied in Li+‐containing organic electrolyte (1 M LiClO4 in propylene carbonate) by galvanostatic charge/discharge and cyclic voltammetry in a three‐electrode configuration. Na6V10O28 electrodes exhibit high specific capacitances of up to 354 F g−1. An asymmetric SC with activated carbon as positive electrode and Na6V10O28 as negative electrode is fabricated and exhibits a high energy density of 73 Wh kg−1 with a power density of 312 W kg−1, which successfully demonstrates that Na6V10O28 is a promising electrode material for high‐energy SC applications.
Environmental Reporting: Transparency to Stakeholders or Stakeholder Manipulation? An Analysis of Disclosure Tone and the Role of the Board of Directors
Authors: Arena Claudia , Bozzolan Saverio , Michelon Giovanna 
Published: May 2014

AbstractWe study whether environmental reporting serves as a transparency tool to communicate sound environmental policies to stakeholders or rather as a manipulation tool of stakeholders' perceptions. In particular, we focus on the relationship between environmental disclosure tone and future environmental performance and we furthermore explore the role of the board of directors' monitoring and stakeholder orientation in shaping this relationship. Using a sample of 288 US oil and gas firms, we find that the bias towards positive language does not reflect purely opportunistic managerial reasons, but rather is a transparency tool to signal future environmental performance. In addition, we document that the stakeholder orientation of the board plays a transparency role in communicating the firm's superior performance. Our findings contribute to the debate on whether discretionary strategies in environmental reporting are more about increased transparency or about stakeholder manipulation. Moreover, they help investors and policymakers to interpret managers' language choices. Copyright © 2014 John Wiley & Sons, Ltd and ERP Environment.