bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fish or n3‐PUFA intake and body composition: a systematic review and meta‐analysis
Authors: Bender N. , Portmann M. , Heg Z. , Hofmann K. , Zwahlen M. , Egger M. 
Published: May 2014

SummaryObesity is a major public health issue and an important contributor to the global burden of chronic disease and disability. Studies indicate that fish and omega‐3 polyunsaturated fatty acids (n3‐PUFA) supplements may help prevent cardiovascular and metabolic diseases. However, the effect of fish oil on body composition is still uncertain, so we performed a systematic review of randomized controlled trials and the first meta‐analysis on the association between fish or fish oil intake and body composition measures. We found evidence that participants taking fish or fish oil lost 0.59 kg more body weight than controls (95% confidence interval [CI]: −0.96 to −0.21). Treatment groups lost 0.24 kg m−2 (body mass index) more than controls (−0.40 to −0.08), and 0.49 % more body fat than controls (−0.97 to −0.01). Fish or fish oil reduced waist circumference by 0.81 cm (−1.34 to −0.28) compared with control. There was no difference for fat mass and lean body mass. Further research is needed to confirm or refute our findings and to reveal possible mechanisms by which n3‐PUFAs might reduce weight.
A systematic review of skin closure techniques in primary cleft lip surgery
Authors: Pepper H.B. , Perry R. , Ness A. , Sandy J. , Thomas S. 
Published: May 2014

AbstractAimTo carry out a systematic review of the published evidence for skin closure techniques in primary cleft lip surgery.MethodsFive electronic databases were searched from their inception to February 2013 to identify all relevant studies of wound closure techniques in primary cleft lip surgery. Two reviewers independently determined eligibility of studies, assessed the methodological quality of the included studies and extracted data.ResultsSix eligible studies were included. Five were observational studies so were prone to bias. One was a randomised controlled study. Four compared adhesive with sutures, one compared adhesives with steri‐strips and one compared absorbable and non‐absorbable sutures. No cases of dehiscence (primary outcome measure) were reported. The methodological quality of all the included studies was poor.ConclusionsThere is currently insufficient evidence to guide clinical practice for incision closure in primary cleft lip surgery. Adequately powered randomised controlled trials are required to address this issue.
Three‐dimensional Computerized Preoperative Planning of Total Hip Arthroplasty with High‐Riding Dislocation Developmental Dysplasia of the Hip
Authors: Zeng Yi , Lai Ou‐jie , Shen Bin , Yang Jing , Zhou Zong‐ke , Kang Peng‐de , Pei Fu‐xing , Zhou Xuan 
Published: May 2014

ObjectiveTo assess whether computed tomography (CT)‐based 3‐dimensional (3D) computerized pre‐operative planning is accurate and reliable in patients with high‐riding dislocation developmental dysplasia of the hip (DDH) undergoing total hip arthroplasty (THA).MethodsBetween September 2009 and February 2011, a prospective study with an inbuilt means of comparing predictive techniques in 20 patients (20 hips) with high‐riding dislocation DDH was undertaken. All patients had pre‐ and post‐operative CT scans, data from which were transferred digitally to Mimics software. 3D pre‐operative planning to predict the acetabular component size, hip rotation center position and acetabular component coverage was performed using Mimics software. The results and post‐operative course were compared with those of the traditional acetate templating technique.ResultsUsing 3D computerized planning, 14/20 components (70%) were predicted exactly and 6/20 (30%) within one size, whereas with the conventional acetate templating technique, 5/20 components (25%) were predicted exactly, 9/20 (45%) within one size and 6/20 (30%) within two or more sizes. There was a strong correlation between the 3D computerized planned acetabular component size, hip rotation center distance, acetabular component host coverage and that found postoperatively. Five patients were considered to need structural bone graft on the basis of 3D computerized planning; this was highly coincident with the intraoperative findings in all five cases.ConclusionCT‐based 3D computerized pre‐operative planning using Mimics software is an accurate and reliable technique for patients with high‐riding dislocation DDH undergoing THA.
What is the Rate of Lumbar Adjacent Segment Disease after Percutaneous versus Open Fusion?
Authors: Radcliff Kristen E , Kepler Christopher K , Maaieh Motasem , Anderson D Greg , Rihn Jeffrey , Albert Todd , Vaccaro Alex , Hilibrand Alan 
Published: May 2014

ObjectiveAdjacent segment disease (ASD) requiring treatment or re‐operation is a common problem after surgery on the lumbar spine. The hypothesis of this retrospective study was that ASD occurs less often following lumbar spine fusion in patients who undergo percutaneous minimally invasive (MIS) instrumentation than in those in whom open instrumentation is used.MethodsA case‐control study was performed on consecutive patients who had undergone staged single or two level anterior lumbar interbody fusion for degenerative conditions followed by open or MIS instrumentation from 2002 to 2005 in our institution. ASD was defined as that necessitating additional procedures for new symptoms related to an adjacent lumbar dermatome.ResultsOne hundred and seventeen patients met the inclusion criteria. Of these, 53 had been followed up by chart or medical record review for longer than one year. There were 23 patients in the MIS group and 30 in the open group. Of the 30 patients in the open group, 9 had developed ASD (30%). Of the 23 patients in the MIS group, 7 had developed ASD (30%). This difference is not statistically significant (P = 1.00).ConclusionContrary to our hypothesis, there was no significant difference in incidence of ASD in patients who had underwent open versus percutaneous instrumentation following anterior lumbar interbody fusion.
Biomechanical Analysis of Four Types of Internal Fixation in Subtrochanteric Fracture Models
Authors: Wang Jie , Ma Xin‐long , Ma Jian‐xiong , Xing Dan , Yang Yang , Zhu Shao‐wen , Ma Bao‐yi , Chen Yang , Feng Rui , Jia Hao‐bo , Yu Jing‐tao 
Published: May 2014

ObjectiveTo compare the biomechanical properties of four types of internal fixation (proximal femoral nail [PFN], dynamic hip screw [DHS], dynamic condylar screw [DCS], and proximal femoral locking plate [PFLP]) for different types of subtrochanteric fractures.MethodsThirty‐two antiseptic femurs were randomly divided into four groups. After internal fixation had been implanted, different types of subtrochanteric fracture models were produced and each tested under vertical, torsional and vertical damage loads.ResultsThe stiffness ratio of PFN in each fracture model and failure load were the highest in the four groups; however, the torsional stiffness ratio was the lowest. Tension strain ratios of DHS and DCS on the lateral side became compression strain ratios with restoration of the medial fragment. The stiffness ratio of DHS was lower than PFLP in each fracture model, torsional stiffness ratio was the highest in fracture models II to V and the failure load was lower only than PFN. The stiffness ratio and failure load of DCS were both the lowest, torsional stiffness ratio was similar to PFLP's in fracture models II to V. The stiffness ratio of PFLP was only lower than PFN's in each fracture model, but the failure load was lower than DHS's.ConclusionFour types of internal fixation achieve better stabilities for type I subtrochanteric fractures. PFN and PFLP produce reliable stability in type IIIA subtrochanteric fractures. If the medial buttress is restored, DCS can be considered. For type IV subtrochanteric fractures, only PFN provides stable fixation. PFLP is suitable for comminuted fractures with large fragments.
Chronic N‐Methyl‐d‐Aspartate Receptor Activation Induces Cardiac Electrical Remodeling and Increases Susceptibility to Ventricular Arrhythmias
Authors: SHI SHAOBO , LIU TAO , LI YAFENG , QIN MU , TANG YANHONG , SHEN JERRY Y. , LIANG JINJUN , YANG BO , HUANG CONGXIN 
Published: May 2014

BackgroundN‐Methyl‐d‐aspartate receptors, also known as NMDA Receptors or NMDAR, are glutamate receptors that control calcium ion channels and regulate synaptic plasticity. Acute NMDAR activation can induce ventricular arrhythmias (VAs). However, the influence of chronic NMDAR activation on cardiac electrophysiology remains unknown.Methods and ResultsWistar rats were randomly administered 0.9% saline (CTL group), NMDA (N group), or NMDA plus MK801 (N+M group) for 14 days. Compared with the CTL group, the N group displayed elevated heart rate and prolonged QT, QTc, and TpTe intervals in the electrocardiogram (P < 0.05 for all). Then, the S1S2, S1S1, and Burst pacing were performed to assess the characteristics of repolarization; threshold of action potential duration (APD) alternans; beat‐to‐beat variability of repolarization (BVR); and VAs susceptibility in the left ventricular. The prolonged APD at 90% repolarization (APD90); decreased ERP/APD90; increased dispersion of APD90, ERP, and ERP/APD90; decreased threshold of APD alternans; increased BVR; and incidence of VAs were showed in the N group compared with those of the CTL group (P < 0.01 for all). Moreover, chronic NMDA administration reduced the expression of Kv4.2, Kv4.3, KChIP2, and Kv11.1 proteins, and induced mild myocardial interstitial fibrosis (P < 0.01 for all). Importantly, these alterations induced by NMDA were normalized by co‐treatment with MK801.ConclusionChronic NMDAR activation prolonged repolarization, induced electrical instability, and facilitated VAs, which may be associated with reduced Ito and IKr and myocardial fibrosis.
Clinical, Anatomical, and Technical Risk Factors for Postoperative Pacemaker or Defibrillator Lead Perforation with Particular Focus on Myocardial Thickness
Authors: SCHWERG MARIUS , STOCKBURGER MARTIN , SCHULZE CHRISTOPH , BONDKE HANSJÜRGEN , POLLER WOLFRAM C. , LEMBCKE ALEXANDER , MELZER CHRISTOPH 
Published: May 2014

BackgroundPostoperative lead perforation is a life‐threatening complication of cardiac pacing. Identification of precipitating factors for this serious complication may help to anticipate a specific risk profile and to minimize the incidence.MethodsWe conducted a retrospective tertiary referral center analysis to clarify clinical, anatomical, and technical characteristics related to pacemaker (PM) and cardioverter/defibrillator lead perforation.We examined the baseline characteristics and the symptoms. In a subgroup, we investigated the myocardial thickness on contrast‐enhanced cardiac computed tomography.ResultsWe enrolled 26 patients. Female gender appears to put patients at slightly increased risk for lead perforation. In a majority active fixation leads were used. Symptoms occurred in 72%. Pericardial effusion and tamponade were present in 38% and 19%, respectively. Sensing was compromised in 65%. A high pacing threshold or exit block occurred in 92%. Myocardial thickness did not differ between patients with or without perforation. In 96%, the perforation was treated by transvenous withdrawal.ConclusionChest pain, phrenic stimulation, bad sensing, or exit block early after PM implantation must prompt radiological and echocardiographic evaluation. A missing pericardial effusion particularly late after implantation does not rule out a perforation. Especially active fixating leads have a higher risk of perforation. With cardiac surgery in standby transvenous withdrawal is a safe way to treat lead perforation.
Coexistence of Tuberculosis and Mammary Carcinoma in a Goat
Authors: Quintas H , Alegria N , Mendonça A , Botelho A , Alves A , Pires I 
Published: May 2014

ContentsSynchronic occurrence of tuberculosis mastitis and mammary cancer is rare in humans and, to the best of our knowledge, not reported in domestic animals. Here, we present a case of a female adult goat of Serrana breed with simultaneous occurrence of a granulomatous mastitis, due to Mycobacterium caprae, and a mammary carcinoma. Both pathological conditions are rare in goats and should be included in differential diagnosis of mammary lesions.
Retracted: ATP Content, Oxidative Stress and Motility of Beluga (Huso huso) Semen: Effect of Short‐Term Storage
Authors: Aramli MS 
Published: May 2014

ContentsAn effective technique for short‐term storage of semen is essential when processing multiple sperm samples and when semen must be transported from collection sites to hatcheries for the fertilization of ova or to laboratories for cryopreservation. In this experiment, beluga (Huso huso) sperm were used to evaluate the effects of short‐term storage on several quality parameters (i.e. motility, adenosine triphosphate (ATP) content and oxidative stress indices). Sperm cells exhibited > 50% motility during 3 days of storage with an average total duration of sperm motility varying from 13.33 ± 5.77 to 278.33 ± 25.65 s, and no motile spermatozoa were recorded after 9 days of storage. The levels of oxidative stress indices (thiobarbituric acid reactive substances and carbonyl derivatives of proteins) and antioxidant activity (superoxide dismutase) increased significantly after 3 days of storage. The ATP content also decreased significantly after 2 days of storage. The results of this study can be used to develop effective reproduction management and cryopreservation protocols for this endangered fish.
Genome Sequence of the Novel Reassortant Mammalian Orthoreovirus Strain MRV00304/13, Isolated from a Calf with Diarrhea from the United States
Authors: Anbalagan Srivishnupriya , Spaans Tina , Hause Ben M. 
Published: May 2014

ABSTRACT
Mammalian orthoreovirus (MRV) strain MRV00304/13 was isolated from diarrheic calves. The serotype-specific antigen σ1 was found to be 95% identical to that of bovine MRV1. All predicted viral proteins had >92% identity to those of MRV except µ2 and σ1s (80 and 72% identities, respectively), suggesting that MRV00304/13 is a novel reassortant MRV1.
Draft Genome Sequence of
bla
NDM-1
-Positive Escherichia coli O25b-ST131 Clone Isolated from an Environmental Sample
Authors: Kutumbaka Kirthi K. , Han Sukkyun , Mategko James , Nadala Cesar , Buser Genevieve L. , Cassidy Maureen P. , Beldavs Zintars G. , Weissman Scott J. , Morey Karim E. , Vega Robert , Samadpour Mansour 
Published: May 2014

ABSTRACT

A multidrug-resistant NDM-1 carbapenamase-producing
Escherichia coli
sequence type 131 (ST131) organism was obtained from vacuum cleaner dust collected from the home of a case patient. Here, we report the assembly and annotation of its genome.
Draft Genome Sequence of the Field Isolate Brucella melitensis Strain Bm IND1 from India
Authors: Rao Sashi Bhushan , Gupta Vivek K. , Kumar Mukesh , Hegde Nagendra R. , Splitter Gary A. , Reddanna Pallu , Radhakrishnan Girish K. 
Published: May 2014

ABSTRACT

Brucella
spp. are facultative intracellular bacterial pathogens causing the zoonotic disease brucellosis. Here, we report the draft genome sequence of the
Brucella melitensis
strain from India designated Bm IND1, isolated from stomach contents of an aborted goat fetus.
Draft Genome Sequence of Bacillus thuringiensis Strain LM1212, Isolated from the Cadaver of an
Oryctes gigas
Larva in Madagascar
Authors: Liu Guiming , Deng Chao , Song Lai , Peng Qi , Zhang Jie , Lereclus Didier , Song Fuping 
Published: May 2014

ABSTRACT

We report the draft genome sequence of
Bacillus thuringiensis
strain LM1212, which differentiates into crystal producers or spore formers during the stationary phase. Availability of this genome sequence will facilitate the study of spore formation, crystal formation, cell differentiation, and evolution of
B. thuringiensis
.
Draft Genome Sequence of
Frankia
sp. Strain BMG5.23, a Salt-Tolerant Nitrogen-Fixing Actinobacterium Isolated from the Root Nodules of
Casuarina glauca
Grown in Tunisia
Authors: Ghodhbane-Gtari Faten , Hurst Sheldon G. , Oshone Rediet , Morris Krystalynne , Abebe-Akele Feseha , Thomas W. Kelley , Ktari Amir , Salem Karima , Gtari Maher , Tisa Louis S. 
Published: May 2014

ABSTRACT

Nitrogen-fixing actinobacteria of the genus
Frankia
are symbionts of woody dicotyledonous plants termed actinorhizal plants. We report here a 5.27-Mbp draft genome sequence for
Frankia
sp. strain BMG5.23, a salt-tolerant nitrogen-fixing actinobacterium isolated from root nodules of
Casuarina glauca
collected in Tunisia
.
Drug-induced thrombocytopenia secondary to natalizumab treatment
Authors: Cachia David , Izzy Saef , Berriosmorales Idanis , Ionete Carolina 
Published: May 2014

SummaryA 52-year-old woman with a 10-year history of relapsing-remitting multiple sclerosis (RRMS) was started on natalizumab after she developed side effects for interferon β-1a and glatiramer acetate. The patient presented with acute severe infusion reaction after the third treatment with natalizumab, developing whole-body purpura. Laboratory testing revealed progressive worsening thrombocytopenia up to 3 weeks following natalizumab discontinuation. Platelet antibodies to platelet-specific antigen as well as antibodies against natalizumab were positive. Bone marrow biopsy was negative. The patient was diagnosed with drug-induced immune thrombocytopenia (DITP) as a rare case of natalizumab side effect which was treated with intravenous methylprednisolone followed by rituximab with successful resolution of thrombocytopenia. The patient had a stable course of RRMS with no relapses and no brain MRI changes at 2 years after initiation of rituximab.
Signposting GP trainees to relevant learning opportunities in hospital posts:the Super-condensed Curriculum Guide
Authors: Denney MeiLing 
Published: May 2014

AbstractUK three-year GP specialty training programmes consist of 18 months in hospital posts and 18 months in general practice. Within the hospital setting, clinical supervisors of GP trainees may have difficulty determining which learning opportunities available within the post are most relevant to training for a future career in general practice. Feedback from GP trainees has indicated that there is a lack of consistency in hospital posts regarding relevance of training for general practice.The aim of the project was to provide support to the hospital supervisors in order to improve the overall quality of hospital posts in GPST programmes and their relevance to General Practice training, and to provide guidance to GP trainees to target their learning most effectively within each specialty post to improve relevance to future career. The deanery set out to develop a tool, the Super Condensed Curriculum Guide (SCCG) consisting of a set of documents created for a specialty with involvement from stakeholder groups. It was intended that this guide would stand alongside the relevant part of the GP curriculum. A programme of familiarisation and initial training for clinical supervisors was delivered. Take-up of the clinical supervisor training sessions was not uniform. Following favourable initial feedback from trainee groups and clinical supervisors across the region, the guides were developed for the remaining specialties in the programme.Trainees were also informed about the guide and how it might help focus their learning in a hospital post. Feedback from trainees across the specialties was positive, but more needs to be done to engage clinical supervisors across the range of specialties. This will improve the utility of the tool, help to guide the clinical supervisor in their teaching, and make sure each post is as educationally effective as possible.
BRAFV600 co-testing in thyroid FNA cytology: short-term experience in a large cancer centre in the UK
Authors: Poller David N , Glaysher Sharon , Agrawal Avi , Caldera Saliya , Kim Dae , Yiangou Constantinos 
Published: May 2014

AimsTo ascertain whetherBRAFV600 mutational analysis is useful for diagnosis of thyroid cancer in thyroid fine needle aspirate (FNA).MethodsOver 8 months thyroid FNAs reported as Thy 3F(neoplasm possible/suggestive of follicular neoplasm), Thy4 (suspicious of malignancy) and Thy 5 (malignant) were tested forBRAFV600 mutation and managed as malignant if mutations were present.ResultsOf 207 FNAs from 176 patients, 5 were Thy 5, 19 Thy 4, 36 Thy 3f,13 Thy 3a, 84 Thy 2 and 50 Thy 1. 11 Thy 3f, 15 Thy 4 and 3 Thy 5 FNAs were tested forBRAFV600 mutation. 0 Thy 3F cases, 6 Thy 4 and 1 Thy 5 (24% of the total tested) showed evidence of mutation. Four patients withBRAFV600 mutation underwent surgery to remove all thyroid tissue, two patients received a lobectomy and one patient is awaiting thyroidectomy. All patients withBRAFV600 mutation were found to have malignancy on final histology, with a diagnostic sensitivity for malignancy excluding coincidental microcarcinoma of 43% and specificity of 100%.ConclusionsBRAFV600 mutational analysis can enable single-stage total thyroidectomy for carcinoma if gene mutation is present in preoperative FNA.BRAFV600 co-testing may reduce the need for completion thyroidectomy with implied cost savings and lower patient morbidity associated with completion thyroidectomy when the cytology is inconclusive but whereBRAFV600 mutation is identified in preoperative thyroid FNA.
MANAGEMENT OF FLEXOR TENDON INJURIES — PART 2: CURRENT PRACTICE IN AUSTRALIA AND GUIDELINES FOR TRAINING YOUNG SURGEONS
Authors: Tolerton Sarah K. , Lawson Richard D. , Tonkin Michael A. 
Published: May 2014

Introduction: This study aims to gain a better understanding of current practice for the surgical management and rehabilitation of flexor tendon injuries in Australia, with the intent of establishing common guidelines for training of young surgeons.Methods: A survey was distributed to the membership of the Australian Hand Surgery Society to determine whether a consensus could be obtained for: suture material and gauge; core and epitenon suture techniques; sheath and pulley management; and post-operative protocols for primary flexor tendon repair.Results: The predominant materials used for core suture are TicronTMSuture (Tyco Healthcare Group LP, Norwalk, Connecticut, USA) (34%) and EthibondTMPolyester Suture (Ethicon, Somerville, New Jersey, USA) (24%). The two core suture configurations commonly used are the Adelaide (45%) and Kessler (32%) repair. The predominant materials used for epitendinous sutures are 6-0 ProleneTMPolypropylene Suture (Ethicon, Somerville, New Jersey, USA) (56%), 5-0 ProleneTM(21%) and 6-0 EthilonTMNylon Suture (Ethicon, Somerville, New Jersey, USA) (13%); and the majority (63%) use a running epitendinous technique. The management of critical pulleys is variable, with 89% prepared to perform some release of A2 and A4 pulleys. Rehabilitation protocols vary widely, with 24% of respondents using the same method for all patients, while 76% tailor their approach to each patient. Some component of active motion was used by most.Discussion: There exists some consensus on the management of flexor tendon injuries in Australia. However, the management of critical pulleys and methods of post-operative rehabilitation remain varied. For the training of young surgeons, a majority advise a 3-0 gauge braided polyester core suture of four strands, combined with a 6-0 ProleneTMsimple running epitendinous suture for increased tendon repair strength and smooth glide. Trainees should attempt to retain the integrity of the A2 and A4 pulleys. Post-operative rehabilitation should include a component of active flexion.