bluuebunny/crossref_metadata_2025_split huggingface dataset:
Studies on properties of the xylan-binding domain and linker sequence of xylanase XynG1-1 from Paenibacillus campinasensis G1-1
Authors: Liu Yihan , Huang Lin , Li Weiguo , Guo Wei , Zheng Hongchen , Wang Jianling , Lu Fuping 
Published: October 2015

Abstract
Xylanase XynG1-1 from Paenibacillus campinasensis G1-1 consists of a catalytic domain (CD), a family 6_36 carbohydrate-binding module which is a xylan-binding domain (XBD), and a linker sequence (LS) between them. The structure of XynG1-3 from Bacillus pumilus G1-3 consists only of a CD. To investigate the functions and properties of the XBD and LS of XynG1-1, two truncated forms (XynG1-1CDL, XynG1-1CD) and three fusion derivatives (XynG1-3CDL, XynG1-3CDX and XynG1-3CDLX) were constructed and biochemically characterized. The optimum conditions for the catalytic activity of mutants of XynG1-1 and XynG1-3 were 60 °C and pH 7.0, and 55 °C and pH 8.0, respectively, the same as for the corresponding wild-type enzymes. XynGs with an XBD were stable over a broad temperature (30–80 °C) and pH range (4.0–11.0), respectively, on incubation for 3 h. Kinetic parameters (K m, k cat, k cat/K m) of XynGs were determined with soluble birchwood xylan and insoluble oat spelt xylan as substrates. XynGs with the XBD showed better affinities toward, and more efficient catalysis of hydrolysis of the insoluble substrate. The XBD had positive effects on thermostability and pH stability and a crucial function in the ability of the enzyme to bind and hydrolyze insoluble substrate. The LS had little effect on the overall stability of the xylanase and no relationship with affinities for soluble and insoluble substrates or catalytic efficiency.
SAUDI PETRODOLLARS, SPIRITUAL CAPITAL, AND THE ISLAMIC UNIVERSITY OF MEDINA: A WAHHABI MISSIONARY PROJECT IN TRANSNATIONAL PERSPECTIVE
Authors: Farquhar Michael 
Published: October 2015

AbstractWhile the idea that Saudi Arabia has functioned as a beacon of ultraconservative religious influence since the 1970s oil boom is commonplace, the modalities of this influence have rarely been seriously interrogated. As a window onto this issue, this article considers the history of the Islamic University of Medina, an influential Wahhabi missionary project with global ambitions. It pays particular attention to the role played by non-Saudi staff, who for long periods made up a majority of the university's faculty. Previous accounts of migrants working in Saudi religious educational institutions have tended to focus on the contested question of their contribution to the rise of politically activist modes of Islamism within the kingdom. In contrast, the account offered here draws on the concept of spiritual capital to argue that they also played an important part in legitimizing the expansion of the Wahhabi mission to diverse Muslim communities around the world.
WOMEN'S VISIBILITY IN PETITIONS FROM GREATER SYRIA DURING THE LATE OTTOMAN PERIOD
Authors: Zachs Fruma , Ben-Bassat Yuval 
Published: October 2015

AbstractThis article focuses on petitions by Ottoman women from Greater Syria during the late Ottoman era. After offering a general overview of women's petitions in the Ottoman Empire, it explores changes in women's petitions between 1865 and 1919 through several case studies. The article then discusses women's “double-voiced” petitions following the empire's defeat in World War I, particularly those submitted to the King-Crane Commission. The concept of “double-voiced” petitions, or speaking in a voice that reflects both a dominant and a muted discourse, is extended here from the genre of literary fiction to Ottoman women's petitions. We argue that in Greater Syria double-voiced petitions only began to appear with the empire's collapse, when women both participated in national struggles and strove to protect their rights as women in their own societies.
The nature of anhedonia and avolition in patients with first-episode schizophrenia
Authors: Lui S. S. Y. , Liu A. C. Y. , Chui W. W. H. , Li Z. , Geng F. , Wang Y. , Heerey E. A. , Cheung E. F. C. , Chan R. C. K. 
Published: October 2015

BackgroundPatients with schizophrenia have intact ability to experience emotion, but empirical evidence suggests that they fail to translate emotional salience into effortful behaviour. Previous research in patients with chronic schizophrenia suggests that working memory is important in integrating emotion and behaviour. This study aimed to examine avolition and anhedonia in patients with first-episode schizophrenia and clarify the role of working memory in emotion–behaviour coupling.MethodWe recruited 72 participants with first-episode schizophrenia and 61 healthy controls, and used a validated emotion-inducing behavioural paradigm to measure participants' affective experiences and how experienced emotion coupled with behaviour. Participants were given the opportunity to expend effort to increase or decrease their exposure to emotion-inducing photographs. Participants with schizophrenia having poor working memory were compared with those with intact working memory in their liking and emotion–behaviour coupling.ResultsPatients with first-episode schizophrenia experienced intact ‘in-the-moment’ emotion, but their emotion was less predictive of the effort expended, compared with controls. The emotion–behaviour coupling was significantly weaker in patients with schizophrenia with poor working memory than in those with intact working memory. However, compared with controls, patients with intact working also showed substantial emotion–behaviour decoupling.ConclusionsOur findings provide strong evidence for emotion–behaviour decoupling in first-episode schizophrenia. Although working memory deficits contribute to defective translation of liking into effortful behaviour, schizophrenia alone affects emotion–behaviour coupling.
Kα emission and secondary electrons in femtosecond laser target interactions
Authors: Nardi Eran , Zinamon Zeev , Maron Yitzhak 
Published: October 2015

AbstractThis paper deals with the contribution of secondary electron emission, produced during the slowing down of fast electrons, on the intensity and temporal shape of the generated Kα pulse. The problem is treated in a general manner emphasizing laser–plasma interactions, where it was suggested in the literature that these electrons could play an important role on the temporal duration. Here, we make use of a hybrid model which includes secondary emission in conjunction with the continuous slowing down approximation (CSDA). The results are compared with those obtained from a simple CSDA calculation, with no detailed accounting of secondary emission and without straggling. Secondary electrons were calculated to contribute up to an additional 20% to the total Kα yield and in the case of monoenergetic electron beams in thick targets also to influence the temporal shape. The pulse duration is not affected in a significant manner by the secondary electrons.
Parenting in the face of childhood life-threatening conditions: The ordinary in the context of the extraordinary
Authors: Mooney-Doyle Kim , Deatrick Janet A. 
Published: October 2015

AbstractObjective:Uncovering what it means to be a parent during the extraordinary time of a child's life-threatening condition (LTC) is important for understanding family goals, decision making, and the work of parenting within this context.Method:Qualitative descriptive methods were employed to describe the everyday experience of parenting both children who have an LTC and their healthy siblings.Results:Some 31 parents of 28 children with an LTC who have healthy siblings participated in our study. Four themes emerged from the data that describe a parental desire to maintain emotional connection with all of their children, how parents use cues from their children to know them better and develop parenting strategies, how parents change as a result of caring for a child with an LTC, and how they strive to decrease suffering for all of their children.Significance of results:The findings of our study have implications for clinical practice, family-focused research, and health policy pertaining to families of children with life-threatening conditions.
Comparing counseling and dignity therapies in home care patients: A pilot study
Authors: Rudilla David , Galiana Laura , Oliver Amparo , Barreto Pilar 
Published: October 2015

AbstractObjective:Several studies have successfully tested psychosocial interventions in palliative care patients. Counseling is the technique most often employed. Dignity therapy (DT) has recently emerged as a tool that can be utilized to address patients' needs at the end of life. The aims of our study were to examine the effects of DT and counseling and to offer useful information that could be put into practice to better meet patients' needs.Method:We developed a pilot randomized controlled trial at the Home Care Unit of the General University Hospital of Valencia (Spain). Some 70 patients were assigned to two therapy groups. The measurement instruments employed included the Patient Dignity Inventory (PDI), the Hospital Anxiety and Depression Scale (HADS), the Brief Resilient Coping Scale (BRCS), the GES Questionnaire, the Duke–UNC-11 Functional Social Support Questionnaire, and two items from the EORTC Quality of Life C30 Questionnaire (EORTC–QLQ–C30).Results:The results of repeated-measures t tests showed statistically significant differences with respect to the dimensions of dignity, anxiety, spirituality, and quality of life for both groups. However, depression increased in the DT group after the intervention, and there were no differences with respect to resilience. Therapy in the counseling group did not negatively affect depression, and resilience did improve. When post-intervention differences between groups were calculated, statistically significant differences in anxiety were found, with lower scores in the counseling group (t(68) = –2.341, p = 0.022, d = 0.560).Significance of Results:Our study provided evidence for the efficacy of dignity therapy and counseling in improving the well-being of palliative home care patients, and it found better results in the counseling therapy group with respect to depression, resilience, and anxiety.
Unpacking the principle of humanity: Tensions and implications
Authors: Fast Larissa 
Published: October 2015

AbstractHumanity is at once the most universally and uncritically accepted humanitarian principle. It is not, however, without controversy. This article defines the principle of humanity and then explores its inherent tensions, related to universality and particularism, inclusion and exclusion, and equality and inequality. The article concludes with a call to operationalize and concretize humanity through three sets of transformative practices and everyday actions. Together these embody the relational nature of humanity, and suggest ways forward in reforming humanitarianism.
Water desalination with a single-layer MoS2 nanopore
Authors: Heiranian Mohammad , Farimani Amir Barati , Aluru Narayana R. 
Published: October 2015

AbstractEfficient desalination of water continues to be a problem facing the society. Advances in nanotechnology have led to the development of a variety of nanoporous membranes for water purification. Here we show, by performing molecular dynamics simulations, that a nanopore in a single-layer molybdenum disulfide can effectively reject ions and allow transport of water at a high rate. More than 88% of ions are rejected by membranes having pore areas ranging from 20 to 60 Å2. Water flux is found to be two to five orders of magnitude greater than that of other known nanoporous membranes. Pore chemistry is shown to play a significant role in modulating the water flux. Pores with only molybdenum atoms on their edges lead to higher fluxes, which are ∼70% greater than that of graphene nanopores. These observations are explained by permeation coefficients, energy barriers, water density and velocity distributions in the pores.
Biofilm-associated bacterial amyloids dampen inflammation in the gut: oral treatment with curli fibres reduces the severity of hapten-induced colitis in mice
Authors: Oppong Gertrude O , Rapsinski Glenn J , Tursi Sarah A , Biesecker Steven G , Klein-Szanto Andres J P , Goulian Mark , McCauley Christine , Healy Catherine , Wilson R Paul , Tükel Cagla 
Published: October 2015

Abstract
Background/objectives:
A disruption of epithelial barrier function can lead to intestinal inflammation. Toll-like receptor (TLR) 2 activation by microbial products promotes intestinal epithelial integrity and overall gut health. Several bacterial species, including enteric bacteria, actively produce amyloid proteins as a part of their biofilms. Recognition of amyloid fibres found in enteric biofilms, termed curli, by the Toll-like receptor (TLR)2/1 complex reinforces barrier function. Here, we investigated the effect of purified curli fibres on inflammation in a mouse model of acute colitis.

Methods:
Bone marrow–derived macrophages as well as lamina propria cells were treated with curli fibres of both pathogenic Salmonella enterica serovar Typhimurium and commensal Escherichia coli Nissle 1917 biofilms. Mice were given 0.1 or 0.4 mg of purified curli orally 1 day post administration of 1% 2,4,6-trinitrobenzene sulphonic acid (TNBS) enema. Histopathological analysis was performed on distal colonic tissue taken 6 days post TNBS enema. RNA extracted from colonic tissue was subjected to RT-PCR.

Results:
Here we show that curli fibres of both pathogenic and commensal bacteria are recognised by TLR2 leading to the production of IL-10, immunomodulatory cytokine of intestinal homeostasis. Treatment of mice with a single dose of curli heightens transcript levels of Il10 in the colon and ameliorates the disease pathology in TNBS-induced colitis. Curli treatment is comparable to the treatment with anti-tumour necrosis factor alpha (anti-TNFα) antibodies, a treatment known to reduce the severity of acute colitis in humans and mice.

Conclusion:
These results suggest that the bacterial amyloids had a role in helping to maintain immune homeostasis in the intestinal mucosa via the TLR2/IL-10 axis. Furthermore, bacterial amyloids may be a potential candidate therapeutic to treat intestinal inflammatory disorders owing to their remarkable immunomodulatory activity.
Diversity and distribution of lichen-associated fungi in the Ny-Ålesund Region (Svalbard, High Arctic) as revealed by 454 pyrosequencing
Authors: Zhang Tao , Wei Xin-Li , Zhang Yu-Qin , Liu Hong-Yu , Yu Li-Yan 
Published: October 2015

AbstractThis study assessed the diversity and distribution of fungal communities associated with seven lichen species in the Ny-Ålesund Region (Svalbard, High Arctic) using Roche 454 pyrosequencing with fungal-specific primers targeting the internal transcribed spacer (ITS) region of the ribosomal rRNA gene. Lichen-associated fungal communities showed high diversity, with a total of 42,259 reads belonging to 370 operational taxonomic units (OTUs) being found. Of these OTUs, 294 belonged to Ascomycota, 54 to Basidiomycota, 2 to Zygomycota and 20 to unknown fungi. Leotiomycetes, Dothideomycetes and Eurotiomycetes were the major classes, whereas the dominant orders were Helotiales, Capnodiales and Chaetothyriales. Interestingly, most fungal OTUs were closely related to fungi from various habitats (e.g., soil, rock, plant tissues) in the Arctic, Antarctic and alpine regions, which suggests that living in association with lichen thalli may be a transient stage of life cycle for these fungi and that long-distance dispersal may be important to the fungi in the Arctic. In addition, host-related factors shaped the lichen-associated fungal communities in this region. Taken together, these results suggest that lichens thalli act as reservoirs of diverse fungi from various niches, which may improve our understanding of fungal evolution and ecology in the Arctic.
Enhanced regeneration and functional recovery after spinal root avulsion by manipulation of the proteoglycan receptor PTPσ
Authors: Li Heng , Wong Connie , Li Wen , Ruven Carolin , He Liumin , Wu Xiaoli , Lang Bradley T. , Silver Jerry , Wu Wutian 
Published: October 2015

AbstractFollowing root avulsion, spinal nerves are physically disconnected from the spinal cord. Severe motoneuron death and inefficient axon regeneration often result in devastating motor dysfunction. Newly formed axons need to extend through inhibitory scar tissue at the CNS-PNS transitional zone before entering into a pro-regenerative peripheral nerve trajectory. CSPGs are dominant suppressors in scar tissue and exert inhibition via neuronal receptors including PTPσ. Previously, a small peptide memetic of the PTPσ wedge region named ISP (Intracellular Sigma Peptide) was generated and its capabilities to target PTPσ and relieve CSPG inhibition were validated. Here, we demonstrate that after ventral root avulsion and immediate re-implantation, modulation of PTPσ by systemic delivery of ISP remarkably enhanced regeneration. ISP treatment reduced motoneuron death, increased the number of axons regenerating across scar tissue, rebuilt healthy neuromuscular junctions and enhanced motor functional recovery. Our study shows that modulation of PTPσ is a potential therapeutic strategy for root avulsion.
Quantitative assessment of common genetic variations in HLA-DP with hepatitis B virus infection, clearance and hepatocellular carcinoma development
Authors: Yu Lei , Cheng Yi-ju , Cheng Ming-liang , Yao Yu-mei , Zhang Quan , Zhao Xue-ke , Liu Hua-juan , Hu Ya-xin , Mu Mao , Wang Bi , Yang Guo-zhen , Zhu Li-li , Zhang Shuai 
Published: October 2015

AbstractHepatitis B virus (HBV) infection is the predominant risk factor for chronic hepatitis B (CHB), liver cirrhosis (LC) and hepatocellular carcinoma (HCC). Recently, genome-wide association studies have identified human leukocyte antigen (HLA)-DP polymorphisms (rs3077 and rs9277535) as a new chronic HBV infection susceptibility locus. Since then, the relationship between HLA-DP polymorphisms and various outcomes of HBV infection has been reported. However, the results have been inconclusive. To derive a more precise estimation of the relationship between HLA-DP polymorphisms and various outcomes of HBV infection, a meta-analysis of 62,050 subjects from 29 case-control studies was performed. We found that rs3077 and rs9277535 in HLA-DP significantly decreased HBV infection risks and increased HBV clearance possibility in a dose-dependent manner. In the subgroup analysis by ethnicity, study design and sample size, significant associations were found for these polymorphisms in almost all comparisons. Meanwhile, haplotype analyses of the two polymorphisms revealed a significant association between the combination of these alleles and HBV infection outcomes. However, no significant results were observed in HCC development. Our results further confirm that genetic variants in the HLA-DP locus are strongly associated with reduced HBV infection and increased the likelihood of spontaneous viral clearance.
Dressed Gain from the Parametrically Amplified Four-Wave Mixing Process in an Atomic Vapor
Authors: Zhang Zhaoyang , Wen Feng , Che Junling , Zhang Dan , Li Changbiao , Zhang Yanpeng , Xiao Min 
Published: October 2015

AbstractWith a forward cone emitting from the strong pump laser in a thermal rubidium atomic vapor, we investigate the non-degenerate parametrically amplified four-wave mixing (PA-FWM) process with dressing effects in a three-level “double-Λ” configuration both theoretically and experimentally. By seeding a weak probe field into the Stokes or anti-Stokes channel of the FWM, the gain processes are generated in the bright twin beams which are called conjugate and probe beams, respectively. However, the strong dressing effect of the pump beam will dramatically affect the gain factors both in the probe and conjugate channels and can inevitably impose an influence on the quantum effects such as entangled degree and the quantum noise reduction between the two channels. We systematically investigate the intensity evolution of the dressed gain processes by manipulating the atomic density, the Rabi frequency and the frequency detuning. Such dressing effects are also visually evidenced by the observation of Autler-Townes splitting of the gain peaks. The investigation can contribute to the development of quantum information processing and quantum communications.
Lipids assist the membrane insertion of a BAM-independent outer membrane protein
Authors: Huysmans Gerard H. M. , Guilvout Ingrid , Chami Mohamed , Nickerson Nicholas N. , Pugsley Anthony P. 
Published: October 2015

AbstractLike several other large, multimeric bacterial outer membrane proteins (OMPs), the assembly of the Klebsiella oxytoca OMP PulD does not rely on the universally conserved β-barrel assembly machinery (BAM) that catalyses outer membrane insertion. The only other factor known to interact with PulD prior to or during outer membrane targeting and assembly is the cognate chaperone PulS. Here, in vitro translation-transcription coupled PulD folding demonstrated that PulS does not act during the membrane insertion of PulD and engineered in vivo site-specific cross-linking between PulD and PulS showed that PulS binding does not prevent membrane insertion. In vitro folding kinetics revealed that PulD is atypical compared to BAM-dependent OMPs by inserting more rapidly into membranes containing E. coli phospholipids than into membranes containing lecithin. PulD folding was fast in diC14:0-phosphatidylethanolamine liposomes but not diC14:0-phosphatidylglycerol liposomes and in diC18:1-phosphatidylcholine liposomes but not in diC14:1-phosphatidylcholine liposomes. These results suggest that PulD efficiently exploits the membrane composition to complete final steps in insertion and explain how PulD can assemble independently of any protein-assembly machinery. Lipid-assisted assembly in this manner might apply to other large OMPs whose assembly is BAM-independent.
Adeno-associated-virus-mediated transduction of the mammary gland enables sustained production of recombinant proteins in milk
Authors: Wagner Stefan , Thresher Rosemary , Bland Ross , Laible Götz 
Published: October 2015

AbstractBiopharming for the production of recombinant pharmaceutical proteins in the mammary gland of transgenic animals is an attractive but laborious alternative compared to mammalian cell fermentation. The disadvantage of the lengthy process of genetically modifying an entire animal could be circumvented with somatic transduction of only the mammary epithelium with recombinant, replication-defective viruses. While other viral vectors offer very limited scope for this approach, vectors based on adeno-associated virus (AAV) appear to be ideal candidates because AAV is helper-dependent, does not induce a strong immune response and has no association with disease. Here, we sought to test the suitability of recombinant AAV (rAAV) for biopharming. Using reporter genes, we showed that injected rAAV efficiently transduced mouse mammary cells. When rAAV encoding human myelin basic protein (hMBP) was injected into the mammary glands of mice and rabbits, this resulted in the expression of readily detectable protein levels of up to 0.5 g/L in the milk. Furthermore we demonstrated that production of hMBP persisted over extended periods and that protein expression could be renewed in a subsequent lactation by re-injection of rAAV into a previously injected mouse gland.