bluuebunny/crossref_metadata_2025_split huggingface dataset:
CD4+ but not CD8+ T cells revert the impaired emotional behavior of immunocompromised RAG-1-deficient mice
Authors: Rattazzi L , Piras G , Ono M , Deacon R , Pariante C M , D'Acquisto F 
Published: July 2013

AbstractAn imbalanced immune system has long been known to influence a variety of mood disorders including anxiety, obsessive-compulsive disorders and depression. In this study, we sought to model the impact of an immunocompromised state on these emotional behaviors using RAG-1−/− mice, which lack T and B cells. We also investigated the relative contribution of CD4+ or CD8+ T cells to these manifestations using RAG-1−/−/OT-II and RAG-1−/−/OT-I transgenic mice, respectively. Our results show that RAG-1−/− mice present a significant increase in digging and marble-burying activities compared with wild-type mice. Surprisingly, these anxiety-like behaviors were significantly reverted in RAG-1−/−/OT-II but not RAG-1−/−/OT-I transgenic mice. Immunodepletion experiments with anti-CD4 or anti-CD8 in C57/BL6 mice or repopulation studies in RAG-1−/− mice did not reproduce these findings. Microarray analysis of the brain of RAG-1−/− and RAG-1−/−/OT-II mice revealed a significantly different gene fingerprint, with the latter being more similar to wild-type mice than the former. Further analysis revealed nine main signaling pathways as being significantly modulated in RAG-1−/− compared with wild-type mice. Taken together, these results suggest that life-long rather than transient immunodeficient conditions influence the emotional behaviors in mice. Most interestingly, these effects seem to correlate with a specific absence of CD4+ rather than CD8+ T cells. Validation of these findings in man might provide new clues on the mechanism by which early life immune modulation might impact mood response in adults and provide a further link between immune and emotional well-being.
Geminin deploys multiple mechanisms to regulate Cdt1 before cell division thus ensuring the proper execution of DNA replication
Authors: Ballabeni Andrea , Zamponi Raffaella , Moore Jodene K. , Helin Kristian , Kirschner Marc W. 
Published: July 2013

Significance
The master cell-cycle processes governing DNA replication and mitosis in eukaryotic cells are regulated by cyclin/cyclin dependent kinase 1 and the anaphase-promoting complex, with checkpoint activity on these regulators. It is not these regulators but rather intermediaries that communicate to the processes. Here we show that the protein Geminin acts centrally in controlling DNA replication by ensuring that DNA is replicated during S phase and only once. This paper describes the Geminin “sub-master” regulatory circuit and the central role of Geminin in controlling events of the cell cycle.
Determining the subcellular location of new proteins from microscope images using local features
Authors: Coelho Luis Pedro , Kangas Joshua D. , Naik Armaghan W. , Osuna-Highley Elvira , Glory-Afshar Estelle , Fuhrman Margaret , Simha Ramanuja , Berget Peter B. , Jarvik Jonathan W. , Murphy Robert F. 
Published: July 2013

Abstract
Motivation: Evaluation of previous systems for automated determination of subcellular location from microscope images has been done using datasets in which each location class consisted of multiple images of the same representative protein. Here, we frame a more challenging and useful problem where previously unseen proteins are to be classified.
Results: Using CD-tagging, we generated two new image datasets for evaluation of this problem, which contain several different proteins for each location class. Evaluation of previous methods on these new datasets showed that it is much harder to train a classifier that generalizes across different proteins than one that simply recognizes a protein it was trained on.
We therefore developed and evaluated additional approaches, incorporating novel modifications of local features techniques. These extended the notion of local features to exploit both the protein image and any reference markers that were imaged in parallel. With these, we obtained a large accuracy improvement in our new datasets over existing methods. Additionally, these features help achieve classification improvements for other previously studied datasets.
Availability: The datasets are available for download at http://murphylab.web.cmu.edu/data/. The software was written in Python and C++ and is available under an open-source license at http://murphylab.web.cmu.edu/software/. The code is split into a library, which can be easily reused for other data and a small driver script for reproducing all results presented here. A step-by-step tutorial on applying the methods to new datasets is also available at that address.
Contact: murphy@cmu.edu
Supplementary information: Supplementary data are available at Bioinformatics online.
Baclofen for the Treatment of Pediatric GERD
Authors: Vadlamudi Narendra B. , Hitch Meredith C. , Dimmitt Reed A. , Thame Kirk A. 
Published: July 2013

ABSTRACTBackground and Aim:Baclofen, a γ‐aminobutyric acid receptor agonist, has been shown to reduce the episodes of gastroesophageal reflux (GER) by reducing the incidence of transient lower esophageal sphincter relaxations. Although baclofen has been shown to reduce reflux symptoms in adults, data in pediatric patients are limited. The aim of the study was to evaluate the efficacy of baclofen in children with refractory GER.Methods:Medical charts of patients 1 to 18 years of age treated with baclofen for persistent GER symptoms were reviewed retrospectively. Short‐term (at first clinic visit) and long‐term (12 months) clinical responses were assessed.Results:A total of 53 patients were included in the final analysis. The mean duration of illness was 1.5 years and the mean age was 6.1 years. All of the patients were taking either once‐ (53%) or twice‐daily (47%) doses of proton pump inhibitors (PPIs) at the time of initiation of baclofen. Thirty‐five (66%) patients experienced a significant reduction in clinical symptoms at their first follow‐up visit. In the remaining 18 patients, however, baclofen was stopped because of either no response (n = 15) or adverse events (n = 3). A total of 27 patients continued treatment and were assessed for long‐term response. Of those, 22 (81%) had a sustained response to baclofen at 12 months, whereas 5 (19%) lost response. We recognized no clinical characteristic differences between those with and without a response to baclofen at either time point.Conclusions:Baclofen can be used as supplemental therapy to proton pump inhibitors in children with refractory GER; however, prospective trials are needed to further validate our results and assess safety.
How WOM marketing contributes to new product adoption
Authors: López Manuela , Sicilia María 
Published: July 2013

PurposeCommunication strategy is a critical element of new product adoption. The decision to adopt a new product is determined by the success of a sequence of two stages: product awareness and product adoption. Previous studies have shown advertising to be the tool that works best during the first stage of introduction. However, the expansion of new media has facilitated the development and management of WOM campaigns. Recent research has called this strategy WOM marketing (WOMM). The aim of this paper is to determine which communication strategy is more appropriate at the early stages of the diffusion process.Design/methodology/approachTwo between‐subject experimental designs were developed, one with university students and another with a more general population.FindingsContrary to assertions in previous literature, the results show that firms should start new product communication with WOMM and then continue it with advertising.Originality/valueTo the best of the authors' knowledge, this is one of the first studies to analyze whether WOM should be actively promoted by firms, or naturally promoted by customers after an advertising campaign. The research also contributes to the few experimental studies that have been conducted into new product diffusion.
A novel apparel surface flattening algorithm
Authors: Zhuang Meiling , Zhang Xiaofeng 
Published: July 2013

PurposeIn the trend of individuation and customization, more rapid and flexible clothing pattern production systems are required. Many studies about the system have been done into producing paper pattern automatically for sewing. The purpose of this paper is to propose a novel three‐dimensional intelligent pattern‐making algorithm.Design/methodology/approachBody features are referenced for crack designing, the concept of functional dividing is proposed on the triangled upper body surface based on Gauss Curvature. A new surface flattening algorithm based on body features (SFABF) is put forward. Robert Hooke Law and Young's modulus are referenced for energy model (EMRY) setting up to define and calculate the edge length variation of triangle. Basing on EMRY, another optimizing surface flattening algorithm (OSFA) is designed to optimize SFABF so as to minimize the accumulated energy.FindingsShape variation accumulation of flattened pattern can be reduced a lot when the cracks are distributed along functional dividing lines. The points with the largest Gauss Curvature as Bust Point have played a great role in shape variation reduction. Because of textiles' flexibility shape variation need not be reduced to zero. Comparing with the related methods this research is more practical.Originality/valueTo this study, SFABF and OSFA are novel methods to improve practicality. The proposed concept of functional dividing is value to the shape variation reduction from surface flattening.
Application of the TRIZ creativity enhancement approach to the design of a passively adaptive compliant robotic gripper
Authors: Petković Dalibor , Issa Mirna , Pavlović Nenad D. , Zentner Lena 
Published: July 2013

PurposeThe essence of the conceptual design is getting the innovative projects or ideas to ensure the products with best performance. It has been proved that the theory of inventive problem solution (TRIZ) is a systematic methodology for innovation. The purpose of this paper is to illustrate the design of an adaptive robotic gripper as an engineering example to show the significance and approaches of applying TRIZ in getting the creative conceptual design ideas.Design/methodology/approachGripping and holding of objects are key tasks for robotic manipulators. The development of universal grippers able to pick up unfamiliar objects of widely varying shapes and surfaces is a very challenging task. The requirement for new adaptive grippers is the ability to detect and recognize objects in their environments.FindingsThe main aim of this work is to show a systematic methodology for innovation as an effective procedure to enhance the capability of developing innovative products and to overcome the main design problems. The TRIZ method will be utilized in order to eliminate the technical contradictions which appear in the passively adaptive compliant robotic gripper.Originality/valueThe design of an adaptive robotic gripper as an engineering example is illustrated in this paper to show the significance and approaches of applying TRIZ in getting the creative conceptual design ideas.
Production scheduling for injection molding manufacture using Petri Net model
Authors: Wongwiwat Asawin , Bohez Erik L.J. , Pisuchpen Roongrat 
Published: July 2013

PurposeThe purpose of this paper is to propose a new generic hybrid Petri Net (PN) model combined with the lowest makespan cut (LMC) for job shop scheduling problems in mold manufacturing to minimize the makespan of the mold part manufacture schedule.Design/methodology/approachThe LMC algorithm finds a solution close to the optimal solution. The searching of the LMC algorithm starts from the lowest estimated makespan (lowest makespan). Almost all of the lowest makespans (LM) are infeasible makespans. A shifting percentage (SP) is added to the LM to obtain the shifting makespan (SM). The SM is compared with the completion time computed from the reachability tree of the Petri Net (PN) model. If the completion time is greater than the SM, the corresponding branch is cut from the reachability graph, and the SM will be compared with another branch from the reachability tree. There are two scenarios. In the first scenario, there is no feasible solution resulting from the comparison of the completion time and the SM, because the SM is lower than all of the feasible solutions. Therefore, the SP is used to increase the SM. On the contrary, in the second scenario, there is a feasible solution: the SP is used to reduce the SM. In the first scenario, a makespan that is lower than the optimal makespan is found. In the second scenario, a makespan that is greater than the optimal makespan is found. After getting close to bounds of the optimal makespan, the least makespan found in the bounds is the best solution.FindingsThe integration of the Petri Net (PN) model and the LMC algorithm can help to improve the production efficiency. In a case study, the proposed algorithm is being compared with other heuristical methods which are practical examples of mold makespans based on the shortest and the longest processing times. The schedule or the sequence obtained by the proposed algorithm is 30% less than the other methods.Research limitations/implicationsThis research will consider scheduling multiple mold. The mold design and the mold testing phase are not considered.Practical implicationsThe time to produce a mold is very important. Reducing the mold production time will provide more time for mold assembly and testing. The aim of LMC algorithm is minimize the makespan. The time to produce a mold is reduced by finding the best sequence of the jobs and machines.Originality/valueThis paper proposes the new generic hybrid Petri Net model combined with LMC for job shop scheduling problem in the case of mold making shop to optimize the makespan of mold parts scheduling.
Stress among nurses: a multi‐nation test of the demand‐control‐support model
Authors: Baba Vishwanath V. , Tourigny Louise , Wang Xiaoyun , Lituchy Terri , Inés Monserrat Silvia 
Published: July 2013

PurposeThe purpose of this paper is to investigate the effect of job demand, job control, and supervisory support on stress among nurses in China, Japan, Argentina, and the Caribbean using the Job demand‐control (JDC) and the Job demand‐control‐support (JDCS) models.Design/methodology/approachThe authors have employed a comparative research design, cross‐sectional survey methodology with convenient random sampling, and a commonly used statistical analytic strategy.FindingsThe results highlight that job demand, job control, and supervisory support are important variables in understanding stress among nurses. This has been corroborated in China, Japan, Argentina, and the Caribbean. Based on their findings and what is available in the literature, the authors report that the JDCS model has universal significance albeit it works somewhat differently in different contexts.Originality/valueThis study's contribution comes from its comparative nature, theoretical anchor, its use of one of the most popular models of stress, its focus on a profession that is demonstrably stressed, its use of common measures and an established analytic strategy. The study's findings underscore the cross‐cultural usefulness and application of the JDCS model along with its threshold and substitution effects and limiting conditions.
International diversification benefits: an investigation from the perspective of Chinese investors
Authors: Jiang Chonghui , Ma Yongkai , An Yunbi 
Published: July 2013

PurposeThe main purpose of this paper is to investigate whether Chinese investors can benefit from international diversification and where these benefits are to be found.Design/methodology/approachThis paper applies an expanding optimization procedure, which is different from the econometric methods or Monte Carlo simulations adopted in many empirical investigations in the literature. The authors' analysis is based on various realized portfolios that are set up at different dates in the sample period.FindingsBased on a stream of realized portfolios, the authors show that Chinese investors can gain substantially in terms of risk reduction as they venture into foreign markets, regardless of the region into which they choose to diversify and whether in‐sample or out‐of‐sample performance is evaluated. However, the optimal strategies under consideration cannot achieve higher out‐of‐sample expected returns and risk‐adjusted returns than does the domestic investment.Originality/valueIn contrast with those in the literature, the authors' analysis is based on the out‐of‐sample performance of a series of realized optimal portfolios. Their method can address time‐varying correlations that are ignored in most previous research. In addition, this method not only allows them to analyze sizes of diversification benefits but also enables them to examine the major characteristics of international portfolios to gauge the effectiveness of different diversification strategies.
Key determinants of the successful adoption of new product development methods
Authors: Graner Marc , Mißler‐Behr Magdalena 
Published: July 2013

PurposeIn recent years, scholars have devoted increasing attention to the use of methods in new product development. Although their positive impact on product success has been confirmed by a series of studies, little research has so far been conducted into the determinants of the successful method application. The purpose of this paper is to analyze two key determinants for the successful use of methods.Design/methodology/approachThe paper adopts a structural equation modeling approach and analyzes the subject based on a large empirical sample of 410 product development projects.FindingsThe way the new product development process is formalized and the extent of top management support have a significant influence on the successful application of methods. Both factors lead to the use of more methods overall and drive the more thorough and intensive deployment of the specific methods used.Practical implicationsThe paper shows how firms that are seeking a more methodical approach to new product development can consciously create the right determinants for the successful adoption of methods and by doing that can increase the success rate of their new product development activities.Originality/valueThis research adds to the literature on success factors of new product development in two distinct ways. First, the paper investigates the subject at the level of specific product development projects, based on a large empirical sample and using a structural equation modeling approach, for the first time. Second, the methods investigated were chosen in a systematic, multi step process in which the methods used by several functions involved in new product development were taken into account.
Adoption of “eco‐advantage” by SMEs: emerging opportunities and constraints
Authors: Oxborrow Lynn , Brindley Clare 
Published: July 2013

PurposeA recent study has asserted that businesses need to adopt “eco‐advantage”. This paper aims to explore the viability of small and medium enterprises (SMEs) achieving “eco‐advantage” by exploring their understanding of sustainability issues, how they adopt and innovate in terms of sustainability and the benefits and obstacles they face.Design/methodology/approachThe research approach is exploratory, comprised of 15 SME embedded cases based in the UK. The cases are participants in short interventions in sustainable product and process design as a part of a university knowledge transfer project, representing the overall case. Cases are based on interviews with company participants and collaborating academics, supplemented by documentary and observational evidence.FindingsThe results build on the work on “eco‐advantage” found in a recent study, highlighting marketing, rather than compliance issues as a catalyst for change. The newly aware SME enters a development process which involves cumulative capabilities, gaining a nascent inner confidence, which includes espousing wider sustainable values.Research limitations/implicationsThe results reveal the scope and challenges for SMEs to adopt more sustainable practices, encompassing innovations and a broad set of capabilities. Further research points to the need to monitor benefits as well as inputs in evaluating sustainability improvements and to consider longitudinal business sustainability issues.Originality/valueThe paper informs the emerging debate on sustainability in SMEs, providing a rich source of data to enhance the provision of business support and knowledge transfer activities, where a more holistic and customised approach is required to realise the real environmental and economic benefits accrued from implementing sustainable improvements.
Understanding the impact of technology on firms’ business models
Authors: André Cavalcante Sérgio 
Published: July 2013

PurposeThe purpose of this paper is to identify the impact of a new global positioning technology on firms’ business models.Design/methodology/approachThe empirical setting was a consortium of Danish organizations, established to develop a positioning‐based technology platform as a basis for innovative commercial products and/or services. Three of the consortium companies were selected for case‐study research.FindingsThe main findings were that companies will use the new technology to extend their existing business models, and that the technology platform potentially represents the creation of a new business model for the partner companies in the consortium.Practical implicationsThis paper is important in that it will help companies understand technological impact from a business model perspective, thereby enabling them to manage innovation better by distinguishing between the creation, extension, revision or termination of business models.Originality/valueThe main contribution of this study is its use of the business model perspective to analyse the impact of an emergent technology on companies’ innovation activities. This perspective makes it easier to develop strategic initiatives while managing innovation, and underlines the importance of purposive human action in adopting a proactive behaviour, reducing deterministic views on technological impact.