bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrasound‐Mediated Antibiotic Delivery to In Vivo Biofilm Infections: A Review
Authors: Liu Jamie D. , Van Treeck Kelly E. , Marston William A. , Papadopoulou Virginie , Rowe Sarah E. 
Published: June 2024

AbstractBacterial biofilms are a significant concern in various medical contexts due to their resilience to our immune system as well as antibiotic therapy. Biofilms often require surgical removal and frequently lead to recurrent or chronic infections. Therefore, there is an urgent need for improved strategies to treat biofilm infections. Ultrasound‐mediated drug delivery is a technique that combines ultrasound application, often with the administration of acoustically‐active agents, to enhance drug delivery to specific target tissues or cells within the body. This method involves using ultrasound waves to assist in the transportation or activation of medications, improving their penetration, distribution, and efficacy at the desired site. The advantages of ultrasound‐mediated drug delivery include targeted and localized delivery, reduced systemic side effects, and improved efficacy of the drug at lower doses. This review scrutinizes recent advances in the application of ultrasound‐mediated drug delivery for treating biofilm infections, focusing on in vivo studies. We examine the strengths and limitations of this technology in the context of wound infections, device‐associated infections, lung infections and abscesses, and discuss current gaps in knowledge and clinical translation considerations.
Uracil‐Selective Cross‐Linking in RNA and Inhibition of miRNA Function by 2‐Amino‐6‐vinyl‐7‐deazapurine Deoxynucleosides
Authors: Soemawisastra Nadya , Okamura Hidenori , Abdelhady Ahmed Mostafa , Onizuka Kazumitsu , Ozawa Mamiko , Nagatsugi Fumi 
Published: June 2024

AbstractMicroRNAs (miRNAs) regulate gene expression through RNA interference. Consequently, miRNA inhibitors, such as anti‐miRNA oligonucleotides (AMOs), have attracted attention for treating miRNA overexpression. To achieve efficient inhibition, we developed 2‐amino‐6‐vinylpurine (AVP) nucleosides that form covalent bonds with uridine counterparts in RNA. We demonstrated that mRNA cross‐linked with AVP‐conjugated antisense oligonucleotides with AVP were protected from gene silencing by exogenous miRNA. However, endogenous miRNA function could not be inhibited in cells, probably because of slow cross‐linking kinetics. We recently developed ADpVP, an AVP derivative bearing a 7‐propynyl group – which boasts faster reaction rate than the original AVP. Here, we synthesized dADpVP – a deoxy analog of ADpVP – through a simplified synthesis protocol. Evaluation of the cross‐linking reaction revealed that the reaction kinetics of dADpVP were comparable to those of ADpVP. In addition, structural analysis of the cross‐linked adduct discovered N3 linkage against uridine. Incorporating dADpVP into two types of miRNA inhibitors revealed a marginal impact on AMO efficacy yet improved the performance of target site blockers. These results indicate the potential of cross‐linking nucleosides for indirect miRNA function inhibition.
Oxidation‐Responsive Supramolecular Hydrogels Based on Glucosamine Derivatives with an Aryl Sulfide Group
Authors: Ali Onaza , Okumura Bioru , Shintani Yuki , Sugiura Shintaro , Shibata Aya , Higashi Sayuri L. , Ikeda Masato 
Published: June 2024

AbstractSupramolecular hydrogels can be obtained via self‐assembly of small molecules in aqueous environments. In this study, we describe the development of oxidation‐responsive supramolecular hydrogels comprising glucosamine derivatives with an aryl sulfide group. We demonstrate that hydrogen peroxide can induce a gel–sol transition through the oxidation of the sulfide group to the corresponding sulfoxide. Furthermore, we show that this oxidation responsiveness can be extended to photo‐responsiveness with the aid of a photosensitizer.
Electrocatalyst‐Hydrogel Polymer Nanocomposite Thin Films for Water Splitting
Authors: Bovas Anu , Radhakrishnan T. P. 
Published: June 2024

AbstractRealization of scalable technologies for key processes like electrochemical water splitting requires the development of sustainable, efficient and cost‐effective catalytic electrodes. The innumerable electrocatalysts being developed in contemporary research rely on the use of a limited choice of binders for effective deployment in the electrolysis setting. Recent studies have demonstrated the utility of electrically insulating hydrogel polymers as suitable matrices for incorporating the electrocatalyst nanoparticles; the nanocomposite thin films coated on conducting substrates mediate highly efficient electrocatalytic water splitting reactions through the effective confinement of the electrolyte within and release of the gaseous products. After a brief introduction to the basic challenges in the field and the existing approaches that address them, this article outlines the promise of hydrogel based nanocomposite thin films in developing efficient and sustainable catalytic electrodes for water splitting, and their functional role in enhancing the efficiency of the electrochemical process. Directions for further development in this domain are noted.
Spiritual and religious competency training for mental health care professionals: How much is enough?
Authors: Vieten Cassandra , Fox Jesse , Oxhandler Holly K. , Pearce Michelle , Polson Edward C. , Pargament Kenneth , Wong Serena , Currier Joseph M. 
Published: June 2024

AbstractSpirituality and religion (R/S) are important aspects of diversity and functioning for most people, and training in these areas for mental health professionals is needed. But how much training is enough? This paper proposes R/S training guidelines that can be used to inform coursework, practicum/internship didactic training, and supervision.
Bioequivalence of Recombinant Human Teriparatide Injection in Healthy Adult Female Subjects in the Fasting State
Authors: Hu Shengling , Wen Yalei , Li Jing , Chen Wenming , Bai Yichuan , Gong Fengyun 
Published: June 2024

AbstractA single‐center, randomized, open, 2‐period, self‐crossover, single‐dose trial was conducted to evaluate the bioequivalence of the test (T) and reference (R) preparations in healthy adult female subjects under fasting conditions. Seventy‐six subjects were enrolled in the study, and subjects were randomly divided into 2 groups at a 1:1 ratio and were administered once per period, with a 4‐day washout period. In each period, plasma drug concentrations, blood calcium changes, and antibodies were determined for pharmacokinetics, pharmacodynamics, and immunogenicity analysis, respectively, and adverse events were recorded for safety analysis. The 90% confidence intervals for the geometric mean ratios (T:R) of maximum plasma concentration, area under the plasma concentration‐time curve from time 0 to the last measurable concentration, and area under the plasma concentration‐time curve from time 0 to infinity were within the predefined bioequivalence criterion of 80%‐125%, indicating bioequivalence between the T and R preparations under fasting conditions. Comparable serum calcium levels demonstrated pharmacodynamics similarity, and no differences were found in immunogenicity profiles. Additionally, the incidence of adverse reactions to the T preparation was 18.4% lower than that of the R preparation (31.6%). This study confirmed the bioequivalence of the T and R preparations under fasting conditions, along with comparable immunogenicity profiles and good safety.
Genesis of Polynitrogen Compounds Employing Silicon Substituted cyclo[18]carbon: A DFT Investigation
Authors: Sen Sobitri , Bag Arijit , Pal Sourav 
Published: June 2024

AbstractActivation of molecular N2 and its catalytic ability to form NH3 using C17Si has been already reported. This current study reports the formation of exclusive polynitrogen clusters (N4 and N5) on the C17Si ring. The clusters are generated using N2 and N3 respectively. Physical and chemical property analyses of the clusters show that the N5 cluster exhibits greater stability than N4. The former is seen to experience reduced molecular strain compared to the latter owing to its co‐planar geometry. The thermodynamic calculations of the systems further show that the formation of the N5 cluster is spontaneous compared to N4 on the C17Si ring.
Enhancing environmental DNA metabarcoding from marine ecosystems: Impact of filter type, storage method, and storage time on the assessment of fish alpha and beta diversity
Authors: Bizzozzero Manuela R. , Altermatt Florian , Cicciarella Riccardo , Walser Jean‐Claude , Willems Erik P. , Krützen Michael 
Published: June 2024

AbstractThe collection of environmental DNA (eDNA) and subsequent metabarcoding are useful tools for assessing marine fish biodiversity noninvasively. It is of particular importance to evaluate biodiversity in regions that are hard to access and thus less well studied. Sampling and preservation methods tailored to the specific circumstances are required. Aquatic eDNA is often captured on filters made of different materials and pore sizes, and subsequently stored under divergent conditions for varying periods of time. Previous studies on multispecies detection in marine systems have primarily focused on capture and extraction effects. Our study, in contrast, examined the effects of filter type, storage method, and storage time on DNA yield, alpha (i.e., ZOTU richness) and beta diversity (i.e., ZOTU composition) recovered from a marine ecosystem in Shark Bay, Western Australia. We compared two different filter types (cellulose‐nitrate filters with pore sizes of 0.45 μm; glass‐fiber filters with pore sizes of 0.1 μm), two storage methods (preservation in Longmire's solution and drying, respectively), various storage times (30–68 days) on two metabarcoding assays using different fish‐specific primers. Our results showed that storage time decreased DNA yield and affected alpha and beta diversity estimates. Cellulose‐nitrate filters stored in Longmire's solution proved to be the best combination with the smallest decrease in DNA yield, no effect on alpha diversity and consistent community compositions. Storing glass‐fiber filters in Longmire's solution led to a decrease in eDNA yield and alpha diversity estimates with increasing storage time. Furthermore, the largest change in beta diversity for each metabarcode was found for glass‐fiber filters regardless of storage method. Our results highlight the importance of considering storage time and interactions between storage method and filter when analyzing eDNA results, especially when storing samples for an extended time period or comparison of samples stored for different durations.
Hybrid data‐driven and physics‐based simulation technique for seismic response evaluation of steel buckling‐restrained braced frames considering brace fracture
Authors: Sadrara Ali , Epackachi Siamak , Imanpour Ali , Kabir Mohammad Zaman 
Published: June 2024

AbstractThis paper proposes a hybrid data‐driven and physics‐based simulation technique for seismic response evaluation of steel Buckling‐Restrained Braced Frames (BRBFs) considering brace fracture. Buckling‐Restrained Brace (BRB) fracture is represented by cumulative plastic deformation capacity. A dataset, consisting of 95 past BRB laboratory tests and 120 simulated BRB responses generated using the finite element method, is first developed. An Artificial Neural Network‐based (ANN) predictive model is then trained using the training dataset to estimate the cumulative plastic deformation of BRBs. The prediction capability of the ANN‐based predictive model is validated using the training dataset and an existing regression‐based predictive model. In the second part of the paper, an hybrid simulation technique combining the data‐driven model and physics‐based numerical modeling is presented to conduct the nonlinear time history analysis, followed by 1) validation against a full‐scale BRBF testing and 2) demonstration of the proposed simulation technique using a six‐story BRBF. The results confirm that the proposed predictive model can predict the BRB fracture with sufficient accuracy. Furthermore, the hybrid data‐driven physics‐based simulation technique can be used as a powerful tool for dynamic analysis of BRBFs considering BRB fracture.
Honoring the authentic voices of Latina/o/e evaluators and the communities we serve
Authors: Aponte‐Soto Lisa , Casillas Wanda , Ali Asma , Maldonado Saúl I. , López‐Cevallos Daniel F. 
Published: June 2024

AbstractThis article provides a summary of the main themes discussed throughout thisNew Directions for Evaluation(NDE) issue. The issue underscores the importance of investing in Latina/o/e talent to increase the pipeline of Latina/o/e evaluators, committed to social justice who are trained in culturally responsive and equitable evaluation (CREE), with a Latina/o/e lens or LatCREE. The contributing authors of this issue offer LatCREE frameworks and approaches that center the voices of Latina/o/e communities to advance humanizing their assets and counter harmful status quo programs and services that are not aligned with the needs of communities and deplete resources. Furthermore, the current issue provides a deeper understanding of the perspectives and experiences of evaluators working with and for Latina/o/e communities. We offer an issue that critically engages voices that have been woefully absent to date, that of the Latina/o/e communities. In that sense, we hope it will spur discussions and raise questions about our role and responsibility as evaluators, to disrupt harmful practices, and to shed light on the issues impacting our communities and the culturally responsive approaches that may be used to address them.
Evaluation in gentrifying Latinx immigrant communities: Lessons learned from culturally responsive evaluation with youth
Authors: Ali Asma M. , León Jovita Murillo , Chávez Noé Rubén 
Published: June 2024

AbstractCulturally responsive evaluation (CRE) efforts highlight the significance of lived experiences of Latinx communities by honoring these voices to inform decision‐making for more equitable support systems and resources. Gentrification disrupts both the physical and social community characteristics, causing shifts in the existing collective community identity and experience. However, CRE projects provide opportunities for marginalized youth community members to engage meaningfully with and better understand these community changes. We describe three projects conducted with community‐based youth researchers in the Mexican‐American US communities of Pilsen (Chicago) and South Los Angeles, exploring community‐personal identity and relationship shifts among evaluators and youth community members. Our article concludes with lessons learned for CRE evaluators working in gentrifying communities.
Metabolic blood profile and response to treatment with the pyruvate kinase activator mitapivat in patients with sickle cell disease
Authors: van Dijk Myrthe J. , Ruiter Titine J. J. , van der Veen Sigrid , Rab Minke A. E. , van Oirschot Brigitte A. , Bos Jennifer , Derichs Cleo , Rijneveld Anita W. , Cnossen Marjon H. , Nur Erfan , Biemond Bart J. , Bartels Marije , Schutgens Roger E. G. , van Solinge Wouter W. , Jans Judith J. M. , van Beers Eduard J. , van Wijk Richard 
Published: June 2024

AbstractMitapivat is an investigational, oral, small‐molecule allosteric activator of pyruvate kinase (PK). PK is a regulatory glycolytic enzyme that is key in providing the red blood cell (RBC) with sufficient amounts of adenosine triphosphate (ATP). In sickle cell disease (SCD), decreased 2,3‐DPG levels increase the oxygen affinity of hemoglobin, thereby preventing deoxygenation and polymerization of sickle hemoglobin. The PK activator mitapivat has been shown to decrease levels of 2,3‐DPG and increase levels of ATP in RBCs in patients with SCD. In this phase 2, investigator‐initiated, open‐label study (https://www.clinicaltrialsregister.eu/ NL8517; EudraCT 2019‐003438‐18), untargeted metabolomics was used to explore the overall metabolic effects of 8‐week treatment with mitapivat in the dose‐finding period. In total, 1773 unique metabolites were identified in dried blood spots of whole blood from ten patients with SCD and 42 healthy controls (HCs). The metabolic phenotype of patients with SCD revealed alterations in 139/1773 (7.8%) metabolites at baseline when compared to HCs (false discovery rate‐adjusted p < 0.05), including increases of (derivatives of) polyamines, purines, and acyl carnitines. Eight‐week treatment with mitapivat in nine patients with SCD altered 85/1773 (4.8%) of the total metabolites and 18/139 (12.9%) of the previously identified altered metabolites in SCD (unadjusted p < 0.05). Effects were observed on a broad spectrum of metabolites and were not limited to glycolytic intermediates. Our results show the relevance of metabolic profiling in SCD, not only to unravel potential pathophysiological pathways and biomarkers in multisystem diseases but also to determine the effect of treatment.
Associations of a multiparameter polymerase chain reaction panel on genital samples in mid‐pregnancy with pregnancy outcomes: A prospective cohort study
Authors: Van Praet Jens T. , Engelen Melissa , Lebbe Barbara , Loccufier Anne , Logghe Hilde , Muyldermans Astrid , Hellemans Jorn , Reynders Marijke 
Published: June 2024

SynopsisResults of a multiparameter PCR on genital samples collected in mid‐pregnancy were not associated with pregnancy outcomes.
Detecting emergence of ruptures in individual layers of the stretched intestinal wall using optical coherence elastography: A pilot study
Authors: Kiseleva Elena B. , Sovetsky Alexander A. , Ryabkov Maksim G. , Gubarkova Ekaterina V. , Plekhanov Anton A. , Bederina Evgeniya L. , Potapov Arseniy L. , Bogomolova Alexandra Y. , Zaitsev Vladimir Y. , Gladkova Natalia D. 
Published: June 2024

AbstractWe report a new application of compression optical coherence elastography (C‐OCE) to monitor the emergence of ruptures in individual layers of longitudinally stretched small‐intestine walls using tissue samples (n = 36) from nine minipigs. Before stretching, C‐OCE successfully estimated stiffness for each intestine‐wall layer: longitudinal muscular layer with serosa, circumferential muscular layer, submucosa and mucosa. In stretched samples, C‐OCE clearly visualized initial stiffening in both muscular layers. By 25% elongation, a sharp stiffness decrease for the longitudinal muscular layer, indicated emergence of tears in all samples. With further stretching, for most samples, ruptures emerged in the circumferential muscular layer and submucosa, while mucosa remained undamaged. Histology confirmed the OCE‐revealed damaging and absence of tissue damage for ~15% elongation. Thus, C‐OCE has demonstrated a high potential for determining the safety tissue‐stretching threshold which afterward may be used intraoperatively to prevent rupture risk in intestinal tissues stretched during various diagnostic/therapeutic procedures.
Drug‐Drug Interaction between Oral Zamicastat and Continuous Epoprostenol Infusion at Steady‐State Conditions in Healthy Subjects
Authors: Fonseca Marlene , Guimarães Andreia , Gama Helena , Magalhães Luís , Henriques Sara Carolina , Silva Nuno , Almeida Luis , Soares‐da‐Silva Patrício 
Published: June 2024

AbstractThis study intended to evaluate the interactions between zamicastat and epoprostenol in healthy human subjects. This was a single‐center, open‐label, two‐period study. In period 1, epoprostenol 8 ng/kg/min was administered alone. In period 2, epoprostenol 8 ng/kg/min was administered following an 8‐day treatment with zamicastat. Since the initial dose of epoprostenol showed to be insufficiently tolerated, it was decreased to 6 ng/kg/min. Blood samples were collected to determine the metabolites of epoprostenol and concentrations of zamicastat and its metabolites. A total of 54 subjects were enrolled and data from 28 subjects were available for pharmacokinetic analysis. The epoprostenol plus zamicastat‐to‐epoprostenol geometric means ratio (GMR) and corresponding 90% confidence interval (CI) for Cav,ss and area under the plasma concentration–time curve from time 0 up to 16 h at steady state (AUC0‐16,ss) of the metabolites of epoprostenol were within the acceptance bioequivalence range (80.00%‐125.00%). The intrasubject coefficient of variation (ISCV) was below 10% for both parameters, on both metabolites. For zamicastat AUC0‐τ,ss, the zamicastat plus epoprostenol‐to‐zamicastat GMR and corresponding 90% CI were within the bioequivalence acceptance range, while for zamicastat Cmax,ss, the lower limit of the 90% CI was slightly below the acceptance range. For zamicastat metabolites, Cmax,ss and AUC0‐τ,ss and the zamicastat plus epoprostenol‐to‐zamicastat GMR were below the acceptance bioequivalence range. ISCV was between 30% and 41% for Cmax,ss and between 21% and 41% for AUC0‐τ,ss, for zamicastat and both metabolites. This study showed that the administration of zamicastat did not significantly modify the cardiovascular effects of epoprostenol and that the interactions between zamicastat and epoprostenol are not expected to be clinically relevant.
Test–retest reliability and follow‐up of muscle magnetic resonance elastography in adults with and without muscle diseases
Authors: De Wel Bram , Huysmans Lotte , Peeters Ronald , Ghysels Stefan , Byloos Kris , Putzeys Guido , Maes Frederik , Dupont Patrick , Claeys Kristl G. 
Published: June 2024

AbstractBackgroundWe investigated the potential of magnetic resonance elastography (MRE) stiffness measurements in skeletal muscles as an outcome measure, by determining its test–retest reliability, as well as its sensitivity to change in a longitudinal follow‐up study.MethodsWe assessed test–retest reliability of muscle MRE in 20 subjects with (n = 5) and without (n = 15) muscle diseases and compared this to Dixon proton density fat fraction (PDFF) and volume measurements. Next, we measured MRE muscle stiffness in 21 adults with Becker muscular dystrophy (BMD) and 21 age‐matched healthy controls at baseline, and after 9 and 18 months. We compared two different methods of analysing MRE data in this study: ‘Method A’ used the stiffness maps generated by the Philips MRE software, and ‘Method B’ applied a custom‐made procedure based on wavelength measurements on the MRE images.ResultsIntraclass correlation coefficients (ICC) of muscle stiffness ranged from good (0.83 for left vastus medialis, P < 0.001) to poor (0.19 for right rectus femoris, P = 0.212) for the examined thigh muscles with Method A, but we did not find a significant test–retest reliability with Method B (P > 0.050 for all). The ICC of muscle PDFF and volume measurements was excellent (>0.90; P < 0.001) for all muscles. At baseline, the average stiffness of all thigh muscles was significantly lower in adults with BMD than in controls for both Method A (−0.2 kPa, P = 0.025) and Method B (−0.6 kPa, P < 0.001). Regardless of which method was used, there was no significant difference in the evolution of muscle stiffness in patients and controls over 18 months.ConclusionsTest–retest reliability of muscle MRE using a simple 2D technique was suboptimal, and did not reliably measure muscle stiffness changes in adults with BMD as compared with controls over 18 months. While the results provide motivation for testing more advanced 3D MRE methods, we conclude that the simple 2D MRE implementation used in this study is not suitable as an outcome measure for characterizing thigh muscle in clinical trials.