bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of Extracellular Vesicle Long Noncoding RNA (linc-VLDLR) in Tumor Cell Responses to Chemotherapy
Authors: Takahashi Kenji , Yan Irene K. , Wood Joseph , Haga Hiroaki , Patel Tushar 
Published: May 2014

Abstract
Hepatocellular cancer (HCC) is a highly treatment-refractory cancer and is also highly resistant to adverse cellular stress. Although cell behavior can be modulated by noncoding RNAs (ncRNA) within extracellular vesicles (EV), the contributions of long noncoding RNAs (lncRNAs) are largely unknown. To this end, the involvement and functional roles of lncRNAs contained within EVs during chemotherapeutic stress in human HCC were determined. Expression profiling identified a subset of lncRNAs that were enriched in tumor cell–derived vesicles released from two different cell lines. Of these, lincRNA-VLDLR (linc-VLDLR) was significantly upregulated in malignant hepatocytes. Exposure of HCC cells to diverse anticancer agents such as sorafenib, camptothecin, and doxorubicin increased linc-VLDLR expression in cells as well as within EVs released from these cells. Incubation with EVs reduced chemotherapy-induced cell death and also increased linc-VLDLR expression in recipient cells. RNAi-mediated knockdown of linc-VLDLR decreased cell viability and abrogated cell-cycle progression. Moreover, knockdown of VLDLR reduced expression of ABCG2 (ATP-binding cassette, subfamily G member 2), whereas overexpression of this protein reduced the effects of VLDLR knockdown on sorafenib-induced cell death. Here, linc-VLDLR is identified as an EV-enriched lncRNA that contributes to cellular stress responses.
Implications: These findings provide new insight into the role of EVs and demonstrate the capacity of lncRNAs to mediate chemotherapeutic stress response in HCC. Mol Cancer Res; 12(10); 1377–87. ©2014 AACR.
Lipoprotein Subfractions Highly Associated With Renal Damage in Familial Lecithin:Cholesterol Acyltransferase Deficiency
Authors: Kuroda Masayuki , Holleboom Adriaan G. , Stroes Erik S.G. , Asada Sakiyo , Aoyagi Yasuyuki , Kamata Kouju , Yamashita Shizuya , Ishibashi Shun , Saito Yasushi , Bujo Hideaki 
Published: May 2014

Objective—

In familial lecithin:cholesterol acyltransferase (LCAT) deficiency (FLD), deposition of abnormal lipoproteins in the renal stroma ultimately leads to renal failure. However, fish-eye disease (FED) does not lead to renal damage although the causative mutations for both FLD and FED lie within the same
LCAT
gene. This study was performed to identify the lipoproteins important for the development of renal failure in genetically diagnosed FLD in comparison with FED, using high-performance liquid chromatography with a gel filtration column.

Approach and Results—
Lipoprotein profiles of 9 patients with LCAT deficiency were examined. Four lipoprotein fractions specific to both FLD and FED were identified: (1) large lipoproteins (>80 nm), (2) lipoproteins corresponding to large low-density lipoprotein (LDL), (3) lipoproteins corresponding to small LDL to large high-density lipoprotein, and (4) to small high-density lipoprotein. Contents of cholesteryl ester and triglyceride of the large LDL in FLD (below detection limit and 45.8±3.8%) and FED (20.7±6.4% and 28.0±6.5%) were significantly different, respectively. On in vitro incubation with recombinant LCAT, content of cholesteryl ester in the large LDL in FLD, but not in FED, was significantly increased (to 4.2±1.4%), whereas dysfunctional high-density lipoprotein was diminished in both FLD and FED.

Conclusions—
Our novel analytic approach using high-performance liquid chromatography with a gel filtration column identified large LDL and high-density lipoprotein with a composition specific to FLD, but not to FED. The abnormal lipoproteins were sensitive to treatment with recombinant LCAT and thus may play a causal role in the renal pathology of FLD.
Cardiac and Kidney Markers for Cardiovascular Prediction in Individuals With Chronic Kidney Disease
Authors: Matsushita Kunihiro , Sang Yingying , Ballew Shoshana H. , Astor Brad C. , Hoogeveen Ron C. , Solomon Scott D. , Ballantyne Christie M. , Woodward Mark , Coresh Josef 
Published: May 2014

Objective—
Traditional predictors suboptimally predict cardiovascular disease (CVD) in individuals with chronic kidney disease (CKD). This study compared 5 nontraditional cardiac and kidney markers on the improvement of cardiovascular prediction among those with CKD.

Approach and Results—

Among 8622 participants aged 52 to 75 years in the Atherosclerosis Risk in Communities (ARIC) Study, cardiac troponin T, N-terminal pro-B-type natriuretic peptide, cystatin C, β
2
-microglobulin, and β-trace protein were compared for improvement in predicting incident CVD after stratifying by CKD status (940 participants with CKD [kidney dysfunction or albuminuria]). During a median follow-up of 11.9 years, there were 1672 CVD events including coronary disease, stroke, and heart failure (336 cases in CKD). Every marker was independently associated with incident CVD in participants with and without CKD. The adjusted hazard ratios (per 1 SD) were larger for cardiac markers than for kidney markers, particularly in CKD (1.61 [95% confidence interval, 1.43–1.81] for cardiac troponin T, 1.50 [1.34–1.68] for N-terminal pro-B-type natriuretic peptide, and <1.26 for kidney markers). Particularly in CKD group, cardiac markers compared with kidney markers contributed to greater c-statistic increment (0.032–0.036 versus 0.012–0.015 from 0.679 with only conventional predictors in CKD and 0.008–0.011 versus 0.002–0.010 from 0.697 in non-CKD) and categorical net reclassification improvement (0.086–0.127 versus 0.020–0.066 in CKD and 0.057–0.077 versus 0.014–0.048 in non-CKD). The superiority of cardiac markers was largely consistent in individual CVD outcomes.

Conclusions—
A greater improvement in cardiovascular prediction was observed for cardiac markers than for kidney markers in people with CKD. These results suggest that cardiac troponin T and N-terminal pro-B-type natriuretic peptide are useful for better CVD risk classification in this population.
The CAVE Score for Predicting Late Seizures After Intracerebral Hemorrhage
Authors: Haapaniemi Elena , Strbian Daniel , Rossi Costanza , Putaala Jukka , Sipi Tuulia , Mustanoja Satu , Sairanen Tiina , Curtze Sami , Satopää Jarno , Roivainen Reina , Kaste Markku , Cordonnier Charlotte , Tatlisumak Turgut , Meretoja Atte 
Published: May 2014

Background and Purpose—
Seizures are a common complication of intracerebral hemorrhage (ICH). We developed a novel tool to quantify this risk in individual patients.

Methods—
Retrospective analysis of the observational Helsinki ICH Study (n=993; median follow-up, 2.7 years) and the Lille Prognosis of InTra-Cerebral Hemorrhage (n=325; 2.2 years) cohorts of consecutive ICH patients admitted between 2004 and 2010. Helsinki ICH Study patients’ province-wide electronic records were evaluated for early seizures occurring within 7 days of ICH and among 7-day survivors (n=764) for late seizures (LSs) occurring >7 days from ICH. A Cox regression model estimating risk of LSs was used to derive a prognostic score, validated in the Prognosis of InTra-Cerebral Hemorrhage cohort.

Results—
Of the Helsinki ICH Study patients, 109 (11.0%) had early seizures within 7 days of ICH. Among the 7-day survivors, 70 (9.2%) patients developed LSs. The cumulative risk of LSs was 7.1%, 10.0%, 10.2%, 11.0%, and 11.8% at 1 to 5 years after ICH, respectively. We created the CAVE score (0–4 points) to estimate the risk of LSs, with 1 point for each of cortical involvement, age <65 years, volume >10 mL, and early seizures within 7 days of ICH. The risk of LSs was 0.6%, 3.6%, 9.8%, 34.8%, and 46.2% for CAVE scores 0 to 4, respectively. The c-statistic was 0.81 (0.76–0.86) and 0.69 (0.59–0.78) in the validation cohort.

Conclusions—
One in 10 patients will develop seizures after ICH. The risk of this adverse outcome can be estimated by a simple score based on baseline variables.
Primary Preventive Potential for Stroke by Avoidance of Major Lifestyle Risk Factors
Authors: Tikk Kaja , Sookthai Disorn , Monni Stefano , Gross Marie-Luise , Lichy Christoph , Kloss Manja , Kaaks Rudolf 
Published: May 2014

Background and Purpose—
Because primary prevention of stroke is a priority, our aim was to assess the primary preventive potential of major lifestyle risk factors for stroke in middle-aged women and men.

Methods—
Among 23 927 persons, 551 (195 women and 356 men) had a first diagnosis of stroke during an average follow-up of 12.7 years. Using Cox proportional hazards models, we estimated the associations of adiposity, smoking, physical activity, alcohol consumption, and diet with risk of developing stroke. A competing risk model built from cause-specific proportional hazards models accounting for concurrent risk of death was used to calculate relative and absolute reductions in stroke occurrences that could have been achieved by maintaining a healthy lifestyle pattern.

Results—
Obesity, smoking, alcohol consumption, diet, and physical inactivity were each identified as modifiable lifestyle risk factors for stroke. About 38% of stroke cases were estimated as preventable through adherence to a healthy lifestyle profile (never smoking, maintaining optimal body mass index and waist circumference, performing physical exercise, consuming a moderate quantity of alcohol, and following a healthy dietary pattern). Age-specific estimates of 5-year incidence rates for stroke in the actual cohort and in a hypothetical, comparable cohort of individuals following a healthy lifestyle would be reduced from 153 to 94 per 100 000 women and from 261 to 161 per 100 000 men for the age group 60 to 65 years.

Conclusions—
Our analysis confirms the strong primary prevention potential for stroke based on avoidance of excess body weight, smoking, heavy alcohol consumption, unhealthy diet, and physical inactivity.
Using Weather Forecasts to Help Manage Meningitis in the West African Sahel
Authors: Pandya Rajul , Hodgson Abraham , Hayden Mary H. , Akweongo Patricia , Hopson Thomas , Forgor Abudulai Adams , Yoksas Tom , Dalaba Maxwell Ayindenaba , Dukic Vanja , Mera Roberto , Dumont Arnaud , McCormack Kristen , Anaseba Dominic , Awine Timothy , Boehnert Jennifer , Nyaaba Gertrude , Laing Arlene , Semazzi Fredrick 
Published: May 2014

Abstract
Understanding and acting on the link between weather and meningitis in the Sahel could help improve vaccine distribution and save lives. People living there know that meningitis epidemics occur in the dry season and end after the start of the rainy season. Integrating and analyzing newly available epidemiological and meteorological data quantified this relationship, showing that that the risk of meningitis epidemics climbed from a background level of 2% to a maximum risk of 25% during the dry season. These data also suggested that, of all meteorological variables, relative humidity has the strongest correlation to cases of meningitis.
Weather acts alongside a complex set of environmental, social, and economic drivers, and a complementary investigation of local and regional knowledge, attitudes, and practices suggested several additional interventions to manage meningitis. These include improved awareness of early meningitis symptoms and vaccinations for farmworkers who migrate seasonally. An economic survey showed that the cost of a single case of meningitis is 3 times the average annual household income, underscoring the need for improved vaccination strategy.
Using these insights, meteorologists and public health workers developed a tool to guide vaccination decisions. Iterative development allowed a multinational team of public health officials to use the tool while guiding its refinement and directed research toward maximum practical use. That meant focusing on predicting areas where high humidity would naturally end epidemics so vaccines could be moved elsewhere. Using this tool and this approach could have prevented an estimated 24,000 cases of meningitis over a 3-yr period.
Complementing Scientific Monsoon Definitions with Social Perception in Bangladesh
Authors: Stiller-Reeve Mathew Alexander , Syed Md. Abu , Spengler Thomas , Spinney Jennifer A. , Hossain Rumana 
Published: June 2014

AbstractThe monsoon onset is a critical event in the Bangladesh calendar, especially for the domestic agricultural sector. Providing information about the monsoon onset for the past, present, and future has potential benefit for a country so vulnerable to changes in climate. But, when does the monsoon start? To produce any scientific information about monsoon onsets, lengths, and withdrawals, we first need to apply a monsoon definition to our data. Choosing a scientific definition is not such a simple exercise in Bangladesh. Different definitions lead to different monsoon onsets and thereby also monsoon lengths. If a climate application aims to provide information about the monsoon onset, then we need to understand how the people who might use this information perceive the monsoon onset. We then need to understand how their perceptions compare with previous scientific work. In this study we carried out a structured questionnaire in six rural regions around Bangladesh and asked the local agriculturists how they defined the monsoon and when they thought it started. It turns out that the agriculturists and previous scientific publications do not necessarily agree. Our results do not undermine previous scientific work on the monsoon in Bangladesh, but they do carry an important message about how we should design, implement, and evaluate climate applications in Bangladesh that encompass the monsoon onset.
Preliminary study on the mechanism of oridonin-induced apoptosis in human squamous cell oesophageal carcinoma cell line EC9706
Authors: Liu Jun-bao , Yue Jing-yu 
Published: May 2014

Objective To study the apoptosis-inducing effect of the Chinese medicine oridonin in the human oesophageal squamous cell carcinoma cell line EC9706, in vitro. Methods The effect of oridonin on cell proliferation was studied using the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Effects on the cell cycle and apoptosis were studied by flow cytometry; effects on intracellular Ca2+ concentration were studied by measuring the fluorescence intensity of a fluorescent Ca2+ probe by laser scanning confocal microscopy. Results The EC9706 cell-proliferation rate decreased with time and oridonin concentration (10–40 µmol/l). The number of cells in G0 and G1 phases increased significantly following exposure to oridonin for 48 and 72 h respectively, and oridonin was shown to be most effective at inducing apoptosis in EC9706 cells at 40 µmol/l. Compared with the control group, all concentrations of oridonin tested (10–40 µmol/l) significantly increased the Ca2+ fluorescence intensity of EC9706 cells. Conclusions Oridonin was shown to inhibit proliferation and induce apoptosis in the human oesophageal squamous cell carcinoma cell line EC9706, in vitro. These data provide preliminary experimental evidence for the anticancer effects of oridonin, which is as a traditional Chinese medicine used to treat various cancers, including oesophageal squamous cell carcinoma. Further studies are required to elucidate the mode of action.
Plasminogen promotes macrophage phagocytosis in mice
Authors: Das Riku , Ganapathy Swetha , Settle Megan , Plow Edward F. 
Published: May 2014

Key PointsPlasminogen influences uptake of apoptotic bodies and immunoglobulin-coated red cells by macrophages in mice. Plasminogen regulates expression of genes involved in macrophage phagocytosis in vivo.
Chemokine treatment rescues profound T-lineage progenitor homing defect after bone marrow transplant conditioning in mice
Authors: Zhang Shirley L. , Wang Xinxin , Manna Sugata , Zlotoff Daniel A. , Bryson Jerrod L. , Blazar Bruce R. , Bhandoola Avinash 
Published: May 2014

Key PointsHoming of T-lineage progenitors to the thymus is reduced after irradiation. Chemokines limit thymic reconstitution after BMT.
Failure-free survival after initial systemic treatment of chronic graft-versus-host disease
Authors: Inamoto Yoshihiro , Flowers Mary E. D. , Sandmaier Brenda M. , Aki Sahika Z. , Carpenter Paul A. , Lee Stephanie J. , Storer Barry E. , Martin Paul J. 
Published: May 2014

Key Points
Failure-free survival is a potentially useful, efficient, and robust basis for interpreting results of initial treatment of chronic GVHD.
Multiparameter flow cytometry for staging of solitary bone plasmacytoma: new criteria for risk of progression to myeloma
Authors: Paiva Bruno , Chandia Mauricio , Vidriales Maria-Belen , Colado Enrique , Caballero-Velázquez Teresa , Escalante Fernando , Garcia de Coca Alfonso , Montes Maria-Carmen , Garcia-Sanz Ramon , Ocio Enrique M. , Mateos Maria-Victoria , San Miguel Jesus F. 
Published: May 2014

Key Points
MFC is a valuable biomarker to discriminate “true” SBP patients from those with “occult” BM clonal PCs and high-risk of progression to MM.
Molecular liaisons between erythropoiesis and iron metabolism
Authors: Kautz Leon , Nemeth Elizabeta 
Published: May 2014

AbstractAlthough most circulating iron in blood plasma is destined for erythropoiesis, the mechanisms by which erythropoietic demand modulates the iron supply (“erythroid regulators”) remain largely unknown. Iron absorption, plasma iron concentrations, and tissue iron distribution are tightly controlled by the liver-produced hormone hepcidin. During the last decade, much progress has been made in elucidating hepcidin regulation by iron and inflammation. This review discusses the less understood mechanisms and mediators of hepcidin suppression in physiologically and pathologically stimulated erythropoiesis.
Altered glutamyl-aminopeptidase activity and expression in renal neoplasms
Authors: Blanco Lorena , Sanz Begoña , Perez Itxaro , Sánchez Clara E , Cándenas M Luz , Pinto Francisco M , Gil Javier , Casis Luis , López José I , Larrinaga Gorka 
Published: May 2014

Abstract

Background
Advances in the knowledge of renal neoplasms have demonstrated the implication of several proteases in their genesis, growth and dissemination. Glutamyl-aminopeptidase (GAP) (EC. 3.4.11.7) is a zinc metallopeptidase with angiotensinase activity highly expressed in kidney tissues and its expression and activity have been associated wtih tumour development.

Methods
In this prospective study, GAP spectrofluorometric activity and immunohistochemical expression were analysed in clear-cell (CCRCC), papillary (PRCC) and chromophobe (ChRCC) renal cell carcinomas, and in renal oncocytoma (RO). Data obtained in tumour tissue were compared with those from the surrounding uninvolved kidney tissue. In CCRCC, classic pathological parameters such as grade, stage and tumour size were stratified following GAP data and analyzed for 5-year survival.

Results
GAP activity in both the membrane-bound and soluble fractions was sharply decreased and its immunohistochemical expression showed mild staining in the four histological types of renal tumours. Soluble and membrane-bound GAP activities correlated with tumour grade and size in CCRCCs.

Conclusions
This study suggests a role for GAP in the neoplastic development of renal tumours and provides additional data for considering the activity and expression of this enzyme of interest in the diagnosis and prognosis of renal neoplasms.
Growth and characterization of Cu(In,Ga)Se2 thin films by nanosecond and femtosecond pulsed laser deposition
Authors: Chen Shih-Chen , Hsieh Dan-Hua , Jiang Hsin , Liao Yu-Kuang , Lai Fang-I , Chen Chyong-Hua , Luo Chih Wei , Juang Jenh-Yih , Chueh Yu-Lun , Wu Kaung-Hsiung , Kuo Hao-Chung 
Published: June 2014

Abstract
In this work, CuIn1 - x
Ga
x
Se2 (CIGS) thin films were prepared by nanosecond (ns)- and femtosecond (fs)-pulsed laser deposition (PLD) processes. Different film growth mechanisms were discussed in perspective of the laser-produced plasmas and crystal structures. The fs-PLD has successfully improved the inherent flaws, Cu2 - x
Se, and air voids ubiquitously observed in ns-PLD-derived CIGS thin films. Moreover, the prominent antireflection and excellent crystalline structures were obtained in the fs-PLD-derived CIGS thin films. The absorption spectra suggest the divergence in energy levels of radiative defects brought by the inhomogeneous distribution of elements in the fs-PLD CIGS, which has also been supported by comparing photoluminescence (PL) spectra of ns- and fs-PLD CIGS thin films at 15 K. Finally, the superior carrier transport properties in fs-PLD CIGS were confirmed by fs pump-probe spectroscopy and four-probe measurements. The present results indicate a promising way for preparing high-quality CIGS thin films via fs-PLD.
Intensify the application of ZnO-based nanodevices in humid environment: O2/H2 plasma suppressed the spontaneous reaction of amorphous ZnO nanowires
Authors: Lai Chun-Yen , Chien Tzu-Chiao , Lin Ting-Yi , Ke Teng , Hsu Shih-Han , Lee Yun-Ju , Su Chien-ying , Sheu Jeng-Tzong , Yeh Ping-Hung 
Published: June 2014

Abstract
In this work, we have demonstrated that amorphous ZnO nanobranches (a-ZnO NBs) could spontaneously react from the crystalline ZnO NWs (c-ZnO NWs) at specific humid environment. The spontaneous reaction mechanism and result can be analyzed by humidity controlling and optical microscope (OM)/scanning electron microscope (SEM)/Kelvin probe force microscopy (KPFM)/transmission electron microscopy (TEM) system. We can make the c-ZnO NWs spontaneous reaction happen at different humid environments and suppress the a-ZnO NBs spontaneous reaction by oxygen/hydrogen plasma surface passivation. The hydrogen plasma surface treatment also can improve the UV sensing sensitivity more than twofold. This work provides the mechanism and methods of the a-ZnO NBs spontaneous growth and offers the passivation treatment for strengthening and enhancing ZnO-based nanodevice application in humid environment and UV light detection, respectively.