bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cu‐Catalyzed Formal Addition of Aliphatic C(sp3)−H Bonds to Alkenes via Phenyl Radical Involved Intermolecular HAT
Authors: Bai Peizhi , Zhang Xin , Su Qinshuang , Xiao Tiebo , Jiang Yubo , Zhang Guoying , Xie Yinjun , Qin Guiping 
Published: July 2024

AbstractA copper catalyzed aerobic formal addition of aliphatic C(sp3)−H bonds to alkenes via a phenyl radical mediated intermolecular HAT process is reported herein, in which the phenylhydrazine not only acts as a HAT reagent precursor, but also as a hydrogen atom donor. This reaction affords an alternative method for the direct formal addition of aliphatic C(sp3)−H bonds to alkenes utilizing air as the radical initiator without additional reductants at ambient temperature. A variety of alkanes and electron‐deficient alkenes are successfully incorporated, furnishing a series of aliphatic C(sp3)−H formal addition products in 20–93% yield. Preliminary mechanistic studies show that the 2‐tetrahydrofuranyl and phenyl radical are formed in this reaction.
Furin Egress from the TGN is Regulated by Membrane‐Associated RING‐CH Finger (MARCHF) Proteins and Ubiquitin‐Specific Protease 32 (USP32) via Nondegradable K33‐Polyubiquitination
Authors: Su Wenqiang , Ahmad Iqbal , Wu You , Tang Lijie , Khan Ilyas , Ye Bowei , Liang Jie , Li Sunan , Zheng Yong‐Hui 
Published: July 2024

AbstractFurin primarily localizes to the trans‐Golgi network (TGN), where it cleaves and activates a broad range of immature proproteins that play critical roles in cellular homeostasis, disease progression, and infection. Furin is retrieved from endosomes to the TGN after being phosphorylated, but it is still unclear how furin exits the TGN to initiate the post‐Golgi trafficking and how its activity is regulated in the TGN. Here three membrane‐associated RING‐CH finger (MARCHF) proteins (2, 8, 9) are identified as furin E3 ubiquitin ligases, which catalyze furin K33‐polyubiquitination. Polyubiquitination prevents furin from maturation by blocking its ectodomain cleavage inside cells but promotes its egress from the TGN and shedding. Further ubiquitin‐specific protease 32 (USP32) is identified as the furin deubiquitinase in the TGN that counteracts the MARCHF inhibitory activity on furin. Thus, the furin post‐Golgi trafficking is regulated by an interplay between polyubiquitination and phosphorylation. Polyubiquitination is required for furin anterograde transport but inhibits its proprotein convertase activity, and phosphorylation is required for furin retrograde transport to produce fully active furin inside cells.
Phosphorescent PdII–PdII Emitter‐Based Red OLEDs with an EQEmax of 20.52%
Authors: Qiao Lige , Kong Xiangjun , Li Kechun , Yuan Lequn , Shen Yunjun , Zhang Yuzhen , Zhou Liang 
Published: July 2024

AbstractThree dinuclear Pd(II) complexes (1, 2, and 3) with intense red phosphorescence at room temperature are here synthesized using strong ligand field strength compounds. All three complexes are characterized by nuclear magnetic resonance, high‐resolution mass spectrometry, and elemental analyses. Complexes 2 and 3 are characterized by single‐crystal X‐ray diffraction. The crystalline data of 2 and 3 reveal complex double‐layer structures, with Pd–Pd distances of 2.8690(9) Å and 2.8584(17) Å, respectively. Furthermore, complexes 1, 2, and 3 show phosphorescence at room temperature in their solid states at the wavelengths of 678, 601, and 672 nm, respectively. In addition, they show phosphorescence at 634, 635, and 582 nm, respectively, in the 2 wt.% (PMMA) films, and phosphorescence at 670, 675, and 589 nm, respectively, in the deoxygenated CH2Cl2 solutions. Among three complexes, complex 1 shows red emission at 634 nm with phosphorescent quantum yield Ф = 67% in the 2 wt.% PMMA film. Furthermore, complex 1‐based organic light‐emitting diode is fabricated using a vapor‐phase deposition process, and their maximum external quantum efficiency reaches 20.52%, which is the highest percentage obtained by using the dinuclear Pd(II) complex triplet emitters with the CIE coordinates of (0.62, 0.38).
A novel ABCC9 variant in a Greek family with Cantu syndrome affecting multiple generations highlights the functional role of the SUR2B NBD1
Authors: Gao Jian , Ververi Athina , Thompson Ellen , Tryon Rob , Sotiriadis Alexandros , Rouvalis Fotios , Grange Dorothy K. , Giannios Christos , Nichols Colin G. 
Published: July 2024

AbstractCantu syndrome (CS) (OMIM #239850) is an autosomal dominant multiorgan system condition, associated with a characteristic facial phenotype, hypertrichosis, and multiple cardiovascular complications. CS is caused by gain‐of‐function (GOF) variants in KCNJ8 or ABCC9 that encode pore‐forming Kir6.1 and regulatory SUR2 subunits of ATP‐sensitive potassium (KATP) channels. A novel heterozygous ABCC9 variant, c.2440G>T; p.Gly814Trp, was identified in three individuals from a four generation Greek family. The membrane potential in cells stably expressing hKir6.1 and hSUR2B with p.Gly814Trp was hyperpolarized compared to cells expressing WT channels, and inside‐out patch‐clamp assays of KATP channels formed with hSUR2B p.Gly814Trp demonstrated a decreased sensitivity to ATP inhibition, confirming a relatively mild GOF effect of this variant. The specific location of the variant reveals an unrecognized functional role of the first glycine in the signature motif of the nucleotide binding domains in ATP‐binding cassette (ABC) protein ion channels.
Does ESG performance impact value and risk‐taking by commercial banks? Evidence from emerging market economies
Authors: Gangwani Mayank , Kashiramka Smita 
Published: July 2024

AbstractThe study examines the impact of environmental, social, and governance (ESG) performance on the value and risk‐taking behavior of listed commercial banks in emerging market economies (EMEs). The study analyzes a comprehensive sample of 178 commercial banks listed in the top 20 emerging markets from 2015 to 2021. The study employs the System generalized method of moments (GMM) with Arellano‐Bond estimation to address the endogeneity issue. The results indicate that banks with higher ESG disclosure scores are linked to reduced risks for commercial banks such as insolvency, leverage, and liquidity risks, in line with shareholder theory. Furthermore, the results demonstrate that sample banks with higher ESG disclosure scores show better accounting (ROE and ROA) and market (Tobin's Q) performance, supporting both agency theory and resource‐based theory. The robustness tests validate the consistency of the results.
Targeting N4‐acetylcytidine suppresses hepatocellular carcinoma progression by repressing eEF2‐mediated HMGB2 mRNA translation
Authors: Liu Hailing , Xu Lei , Yue Shiwei , Su Hongfei , Chen Xing , Liu Qiumeng , Li Hui , Liang Huifang , Chen Xiaoping , He Jiefeng , Ding Zeyang , Zhang Bixiang 
Published: July 2024

AbstractBackgroundN4‐acetylcytidine (ac4C) represents a novel messenger RNA (mRNA) modification, and its associated acetyltransferase N‐acetyltransferase 10 (NAT10) plays a crucial role in the initiation and progression of tumors by regulating mRNA functionality. However, its role in hepatocellular carcinoma (HCC) development and prognosis is largely unknown. This study aimed to elucidate the role of NAT10‐mediated ac4C in HCC progression and provide a promising therapeutic approach.MethodsThe ac4C levels were evaluated by dot blot and ultra‐performance liquid chromatography‐tandem mass spectrometry with harvested HCC tissues. The expression of NAT10 was investigated using quantitative real‐time polymerase chain reaction, western blotting, and immunohistochemical staining across 91 cohorts of HCC patients. To explore the underlying mechanisms of NAT10‐ac4C in HCC, we employed a comprehensive approach integrating acetylated RNA immunoprecipitation and sequencing, RNA sequencing and ribosome profiling analyses, along with RNA immunoprecipitation, RNA pull‐down, mass spectrometry, and site‐specific mutation analyses. The drug affinity responsive targets stability, cellular thermal shift assay, and surface plasmon resonance assays were performed to assess the specific binding of NAT10 and Panobinostat. Furthermore, the efficacy of targeting NAT10‐ac4C for HCC treatment was elucidated through in vitro experiments using HCC cells and in vivo HCC mouse models.ResultsOur investigation revealed a significant increase in both the ac4C RNA level and NAT10 expression in HCC. Notably, elevated NAT10 expression was associated with poor outcomes in HCC patients. Functionally, silencing NAT10 suppressed HCC proliferation and metastasis in vitro and in vivo. Mechanistically, NAT10 stimulates the ac4C modification within the coding sequence (CDS) of high mobility group protein B2 (HMGB2), which subsequently enhances HMGB2 translation by facilitating eukaryotic elongation factor 2 (eEF2) binding to the ac4C sites on HMGB2 mRNA's CDS. Additionally, high‐throughput compound library screening revealed Panobinostat as a potent inhibitor of NAT10‐mediated ac4C modification. This inhibition significantly attenuated HCC growth and metastasis in both in vitro experiments using HCC cells and in vivo HCC mouse models.ConclusionsOur study identified a novel oncogenic epi‐transcriptome axis involving NAT10‐ac4C/eEF2‐HMGB2, which plays a pivotal role in regulating HCC growth and metastasis. The drug Panobinostat validates the therapeutic potential of targeting this axis for HCC treatment.
Aliphatic Ketone Claisen Rearrangement: Troubleshooting the Transetherification Step by Identifying a Stable Acid Catalyst
Authors: Bruce Veera K. , Farshadfar Kaveh , Rolig Aino , Laasonen Kari , Pihko Petri M. 
Published: July 2024

AbstractAfter optimization for retention of catalytic activity, 4‐chlorobenzoic acid emerged as the optimal catalyst for the aliphatic ketone Claisen rearrangement. The optimal catalyst enables a one‐pot, metal‐free, catalytic protocol from allylic alcohols to γ,δ‐unsaturated ketones. The optimized process tolerates a range of substrates, including substituents with acid‐labile protecting groups. Reaction monitoring and DFT studies of the aliphatic ketone Claisen process agree that the ultimate rearrangement step typically has the highest activation barrier.
Does firm‐level exposure to climate change influence inward foreign direct investment? Revealing the moderating role of ESG performance
Authors: Qing Lingli , Dagestani Abd Alwahed , Nam Eun‐Young , Wang Chengqi 
Published: July 2024

AbstractThe impact of climate change on foreign direct investment (FDI) at the corporate level is insufficiently explored, despite extensive discussions on environmental protection. This research utilizes novel panel data (2009–2022) on Chinese A‐share listed firms, using a two‐way fixed‐effect estimator to probe whether and how a firm's exposure to climate change influences the inflow of FDI, while considering the moderating role of environmental, social, and governance (ESG). The results show: (i) firm‐level exposure to climate change harms inward FDI inflow; (ii) host country firms can mitigate the negative effect of exposure to climate change on inward FDI inflow by strengthening their ESG performance; (iii) exposure to climate change primarily impacts FDI inflow through environmental taxes and green investments; (iv) the detrimental effect of climate change risk on incoming FDI is more pronounced in the central and eastern region of China, and among non‐state‐owned firms. These findings provide insightful theoretical and practical implications for addressing SDG 13 (Climate Action) challenges and for attracting multinational investments in developing countries like China.
Advancements in Cosmetics and Aesthetics: A Comprehensive Review
Authors: Goldust Mohamad , Baumann Leslie , Gold Michael H. , Landau Marina 
Published: July 2024

ABSTRACTObjectiveThis article provides a comprehensive overview of recent advancements in cosmetic dermatology and aesthetics. It covers topics including dermal fillers, botulinum toxin (BTX anti‐aging cosmeceuticals, artificial intelligence (AI) applications, and energy‐based devices.MethodsThe review summarizes the current literature on dermal fillers, BTX, cosmeceuticals, AI applications, and energy‐based devices. It explores emerging technologies, new treatment methods, and ongoing research in these areas. The sources of data include peer‐reviewed journals, clinical trials, and expert opinions to provide a thorough understanding of recent developments and future trends.ResultsDermal fillers, particularly hyaluronic acid (HA), are popular in cosmetic treatments, with innovations like Bioorthogonal strategies and thiomer‐based technology emerging. Biostimulators such as poly‐l‐lactic acid and calcium hydroxyapatite promote neo‐collagenesis. BTX remains a cosmetic standard, with new recombinant toxins, hybrid BTXs, and delivery methods enhancing safety and efficacy. Anti‐aging trends include cellular senescence‐targeting treatments like exosomes, NMN, and PDRN, which need further validation. AI improves diagnosis, personalized treatments, and patient consultations but faces data privacy and bias challenges. Advanced energy‐based devices (RF, HIFU, lasers) and treatments like cryolipolysis and MFU‐V enhance skin rejuvenation and body contouring.ConclusionRecent advancements in cosmetic dermatology and aesthetics, including innovative dermal fillers, BTX developments, anti‐aging cosmeceuticals, AI applications, and energy‐based devices, are transforming the field. These innovations provide enhanced treatment options and improved patient outcomes, although further research is necessary to validate efficacy and address emerging challenges. The future of cosmetic dermatology is promising, with ongoing technological advancements driving continuous improvements in aesthetic medicine.
Effects of burrowing crabs on coastal sediments and their functions: A systematic meta‐analysis
Authors: Rinehart Shelby A. , Dybiec Jacob M. , Walker Janet B. , Simpson Lance , Cherry Julia A. 
Published: July 2024

AbstractBurrowing ecosystem engineers, such as termites, crabs, marmots, and foxes, can profoundly affect the biological structure and ecosystem functions of their environments. However, the relative importance of the effects of burrowing engineers on sediments are challenging to predict and are expected to be influenced by engineer density, engineer functional traits (e.g., burrow morphology), and environmental conditions (e.g., geomorphology, vegetation presence). To develop robust hypotheses predicting the impacts of burrowing ecosystem engineers, we conducted a systematic meta‐analysis evaluating the effects of burrowing crabs on sediment properties, nutrient stocks, and ecosystem functions in soft‐sediment coastal habitats (e.g., salt marshes, mangrove forests, tidal flats). Additionally, we tested the impacts of crab burrow density, burrowing crab superfamily (a proxy for crab burrow morphology and diet), and biotic conditions (i.e., vegetation) on the effects of burrowing crab engineers on coastal sediments. Burrowing crabs rework and oxygenate sediments and accelerate rates of nutrient cycling (i.e., nitrification and CO2 flux). However, the magnitude and direction of burrowing crab effects depend on burrowing crab superfamily, the presence of vegetation, and their interaction. Crab burrow density did not consistently predict burrowing engineer effects on sediments. Future efforts need to focus on implementing rigorous manipulative experiments to assess crab ecosystem engineering effects, since methodological variation has hindered efforts to generalize their effects. Our findings suggest that crab engineering effects are predictable across environmental contexts, and understanding the context dependency of crab engineering effects may promote the management and restoration of the critical ecosystem services that are mediated by crab engineers.
A cautionary message on combining physiological thermal limits with macroclimatic data to predict species distribution
Authors: Chevalier Mathieu , Pignard Vincent , Broennimann Olivier , Guisan Antoine 
Published: July 2024

AbstractMacroclimatic data are widely used to estimate the realized environmental niche of species and predict the current or the future spatial distribution of species. Because the realized niche is a subset of the fundamental niche—constrained by biotic interactions and dispersal limitations—proxies of the fundamental niche (e.g., thermal limits obtained from physiological experiments) are sometimes combined with macroclimatic data under the assumption that areas predicted as unsuitable from a realized niche perspective may belong to the species' fundamental niche. However, it is unclear whether this assumption is valid and whether thermal limits can be combined with macroclimatic data. Here, we explored these questions using available physiological thermal limits measured for 151 ectotherms. Specifically, we explored whether physiological thermal limits are larger than observed (realized) thermal limits measured using macroclimatic data, and what would be the effect of considering the physiological niche in addition to the realized niche for current and future predictions. Our results confirm previously raised concerns, as physiological limits can delimit a narrower range of thermal tolerance than the realized niche, particularly at the cold end of the thermal gradient where adaptive and/or facilitative mechanisms could allow species to survive in temperatures below physiological limits. These findings show that combining data on physiological thermal limits with macroclimatic data is dubious and that spatial predictions should be interpreted with caution because data on physiological thermal limits do not fit well with macroclimatic data that do not capture the conditions that organisms experience in the wild. While estimated physiological thermal limits are likely of value to complement species distribution studies, they are likely more useful in biophysical models that account for additional processes including the animal's behavior.
High‐Speed AFM Observation of Electrolytic Hydrogen Nanobubbles During Potential Scanning
Authors: Ohashi Ryuto , Ueda Mikito , Matsushima Hisayoshi 
Published: July 2024

ABSTRACTNano‐sized bubbles (NBs: nanobubbles) have attracted attention in various fields such as physics, engineering, medicine and agriculture for fundamental and practical reasons. Atomic force microscopy (AFM) has revealed the occurrence of NBs and discovered their flattened shape. However, their dynamic behaviours have not yet been discussed much owing to the slow scanning speed. The existence of these energetically unfavourable structures is still controversial owing to the lack of studies on bubble‐like behaviour of NB such as aggregation, growth and dissolution. Recently developed high‐speed AFM (HS‐AFM) can observe nano‐interface phenomena at a speed of 0.5 frame s−1. In this study, HS‐AFM was applied to electrolytic H2 NBs. We successfully observed NB nucleation, growth and dissolution during a potential scan. Image analysis revealed flattened nuclei with heights of less than 10 nm. The NBs remained stable for a short period after the hydrogen evolution stopped, and they rapidly dissolved at the anodic potential. As the potential sweep was repeated, the number of NB nuclei increased. This is the first study showing the dynamic motion of NBs during the potential sweep by AFM. Videos captured by HS‐AFM make NB existence more certain. This research contributes not only to the NB study but also to the clarification of the gas evolution mechanism on electrodes.
A novel denoising autoencoder hybrid network for remaining useful life estimation of lithium‐ion batteries
Authors: Xia Wei , Xu Jinli , Liu Baolei , Duan Huiyun 
Published: July 2024

AbstractMonitoring the health of lithium batteries is a crucial undertaking in ensuring the safe and dependable functioning of electric vehicles. Data‐driven methods have been proved to be an effective method for identifying the complex degradation process of batteries. To augment the precision of predicting the remaining useful life (RUL), this paper introduces a pioneering architecture for a denoising autoencoder (DAE). This architecture integrates a stacked convolutional neural network with subsequent layers of bidirectional gated recurrent units within an encoder–decoder framework. The utilization of the DAE network is employed as a means to effectively capture and represent the intricate and nonlinear knowledge associated with degradation data acquired from measured sources. Simultaneously, the reconstruction loss is incorporated into the total loss to improve the accuracy and generalization of the prediction model. The efficacy of the proposed approach is substantiated through the utilization of data sets sourced from the NASA Ames Prognostics Data Repository. The comparative findings suggest that the proposed approach demonstrates an exceptional ability to achieve precise and robust estimation in predicting the RUL, surpassing other advanced methodologies.
Precision Diagnostics in Myeloid Malignancies: Development and Validation of a National Capture‐Based Gene Panel
Authors: Orsmark‐Pietras Christina , Lyander Anna , Ladenvall Claes , Hallström Björn , Staffas Anna , Awier Hero , Krstic Aleksandra , Baliakas Panagiotis , Barbany Gisela , Håkansson Cecilia Brunhoff , Gellerbring Anna , Hagström Anna , Hellström‐Lindberg Eva , Juliusson Gunnar , Lazarevic Vladimir , Munters Arielle , Pandzic Tatjana , Wadelius Mia , Ås Joel , Fogelstrand Linda , Wirta Valtteri , Rosenquist Richard , Cavelier Lucia , Fioretos Thoas ,   the SciLifeLab Clinical Genomics Platform and Genomic Medicine Sweden
Published: July 2024

ABSTRACTGene panel sequencing has become a common diagnostic tool for detecting somatically acquired mutations in myeloid neoplasms. However, many panels have restricted content, provide insufficient sensitivity levels, or lack clinically validated workflows. We here describe the development and validation of the Genomic Medicine Sweden myeloid gene panel (GMS‐MGP), a capture‐based 191 gene panel including mandatory genes in contemporary guidelines as well as emerging candidates. The GMS‐MGP displayed uniform coverage across all targets, including recognized difficult GC‐rich areas. The validation of 117 previously described somatic variants showed a 100% concordance with a limit‐of‐detection of a 0.5% variant allele frequency (VAF), achieved by utilizing error correction and filtering against a panel‐of‐normals. A national interlaboratory comparison investigating 56 somatic variants demonstrated highly concordant results in both detection rate and reported VAFs. In addition, prospective analysis of 323 patients analyzed with the GMS‐MGP as part of standard‐of‐care identified clinically significant genes as well as recurrent mutations in less well‐studied genes. In conclusion, the GMS‐MGP workflow supports sensitive detection of all clinically relevant genes, facilitates novel findings, and is, based on the capture‐based design, easy to update once new guidelines become available. The GMS‐MGP provides an important step toward nationally harmonized precision diagnostics of myeloid malignancies.