bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epidemiological features and risk factors associated with the spatial and temporal distribution of human brucellosis in China
Authors: Li Yin-Jun , Li Xin-Lou , Liang Song , Fang Li-Qun , Cao Wu-Chun 
Published: November 2013

Abstract

Background
Human brucellosis incidence in China has been increasing dramatically since 1999. However, epidemiological features and potential factors underlying the re-emergence of the disease remain less understood.

Methods
Data on human and animal brucellosis cases at the county scale were collected for the year 2004 to 2010. Also collected were environmental and socioeconomic variables. Epidemiological features including spatial and temporal patterns of the disease were characterized, and the potential factors related to the spatial heterogeneity and the temporal trend of were analysed using Poisson regression analysis, Granger causality analysis, and autoregressive distributed lag (ADL) models, respectively.

Results
The epidemic showed a significantly higher spatial correlation with the number of sheep and goats than swine and cattle. The disease was most prevalent in grassland areas with elevation between 800–1,600 meters. The ADL models revealed that local epidemics were correlated with comparatively lower temperatures and less sunshine in winter and spring, with a 1–7 month lag before the epidemic peak in May.

Conclusions
Our findings indicate that human brucellosis tended to occur most commonly in grasslands at moderate elevation where sheep and goats were the predominant livestock, and in years with cooler winter and spring or less sunshine.
Circulating miR-30a, miR-126 and let-7b as biomarker for ischemic stroke in humans
Authors: Long Guangwen , Wang Feng , Li Huaping , Yin Zhongwei , Sandip Chaugai , Lou Yan , Wang Yan , Chen Chen , Wang Dao Wen 
Published: November 2013

Abstract

Background
Recently, plasma miRNAs have been reported as biomarkers for various diseases. However, the knowledge on the association of plasma miRNAs with ischemic stroke is still lacking. In this study, we investigated whether plasma concentrations of miR-30a, miR-126 and let-7b may be biomarkers for ischemic stroke in humans.

Methods
One hundred ninety seven patients with ischemic stroke were recruited and their blood samples were collected at 24 h, 1 week, 4 weeks, 24 weeks and 48 weeks after symptoms onset, and fifty healthy volunteers were selected as control. Levels of miRNA were quantified by quantitative real-time PCR. Relative expression level of miRNA was calculated using 2-ΔΔct method. The ability to distinguish the ischemic stroke group from control group was characterized by receiver operating characteristic (ROC) curve, and the area under ROC curve (AUC) was calculated.

Results
Circulating miR-30a and miR-126 levels were markedly down-regulated in all patients with ischemic stroke until 24 weeks. However, circulating let-7b was lower in patients with large-vessel atherosclerosis than healthy volunteers, whereas circulating let-7b had higher level in patients with other kinds of ischemic stroke until 24 weeks. Among all patients, circulating miRNAs levels returned to normal 48 weeks after symptom onset. Receiver operating characteristic (ROC) curve analysis showed that the areas under the curve (AUC) of plasma miR-30a were 0.91, 0.91, 0.92 and 0.93, the miR-126 were 0.92, 0.94, 0.93 and 0.92, and let-7b were 0.93, 0.92, 0.92 and 0.91 at 24 h, 1 w, 4 w and 24 w, respectively.

Conclusions
These data suggest that miR-30a, miR-126 and let-7b might be useful biomarkers for ischemic stroke in humans.
Over-expression and characterization of NS3 and NS5A of Hepatitis C virus genotype 3a
Authors: Anwar Muhammad Ikram , Iqbal Mazhar , Yousef Mohammad S , Rahman Moazur 
Published: November 2013

Abstract

Background
Hepatitis C virus (HCV) is a common and leading cause for liver cirrhosis and hepatocellular carcinoma. Current therapies to treat HCV infection are shown to be partially effective and poorly tolerated. Therefore, ample efforts are underway to rationally design therapies targeting the HCV non-structural proteins. Most of the work carried out in this direction has been focusing mainly on HCV genotype 1. Two direct-acting antiviral agents (DAAs) Telaprevir and Boceprevir are being used against genotype 1a infection in combination therapy with interferon and ribavirin. Unfortunately these DAAs are not effective against genotype 3a. Considering the wide spread infection by HCV genotype 3a in developing countries especially South Asia, we have focused on the recombinant production of antiviral drug targets NS3 and NS5A from HCV genotype 3a. These protein targets are to be used for screening of inhibitors.

Results
High-level expression of NS3 and NS5A was achieved at 25°C, using ~1 and 0.5 mM Isopropyl β-D-1-thiogalactopyranoside (IPTG), respectively. Yields of the purified NS3 and NS5A were 4 and 1 mg per liter culture volume, respectively. Although similar amounts of purified NS3 were obtained at 25 and 14°C, specificity constant (K
cat/K
m) was somewhat higher at expression temperature of 25°C. Circular dichroism (CD) and Fourier-transform infrared (FT-IR) spectroscopy revealed that both NS3 and NS5A contain a mixture of alpha-helix and beta-sheet secondary structures. For NS3 protein, percentages of secondary structures were similar to the values predicted from homology modeling.

Conclusions
NS3 and NS5A were over-expressed and using Nickel-affinity method both proteins were purified to ~ 95% purity. Yield of the purified NS3 obtained is four fold higher than previous reports. CD spectroscopy revealed that difference in activity of NS3 expressed at various temperatures is not related to changes in global structural features of the protein. Moreover, CD and FT-IR analysis showed that NS3 and NS5A contain both alpha-helical and beta-sheet structures and for NS5A, the proportion is almost equal. The production of NS3 and NS5A in milligram quantities will allow their characterization by biophysical and biochemical means that will help in designing new strategies to fight against HCV infection.
Stability scheme of ZnO-thin film resistive switching memory: influence of defects by controllable oxygen pressure ratio
Authors: Huang Hsin-Wei , Kang Chen-Fang , Lai Fang-I , He Jr-Hau , Lin Su-Jien , Chueh Yu-Lun 
Published: November 2013

Abstract
We report a stability scheme of resistive switching devices based on ZnO films deposited by radio frequency (RF) sputtering process at different oxygen pressure ratios. I-V measurements and statistical results indicate that the operating stability of ZnO resistive random access memory (ReRAM) devices is highly dependent on oxygen conditions. Data indicates that the ZnO film ReRAM device fabricated at 10% O2 pressure ratio exhibits the best performance. Transmission electron microscopy (TEM) and X-ray diffraction (XRD) of ZnO at different O2 pressure ratios were investigated to reflect influence of structure to the stable switching behaviors. In addition, PL and XPS results were measured to investigate the different charge states triggered in ZnO by oxygen vacancies, which affect the stability of the switching behavior.
Joint network-channel code optimization for wireless sensor networks
Authors: Alaoui Nabih , Meghdadi Vahid , Cances Jean-Pierre 
Published: November 2013

Abstract
Optimization of sensor network architecture in order to improve the overall system performance at the end processing station is an important challenge. A lot of architectures have been designed to optimize the bit error rate. Conventionally, this optimization is done by inserting relays between sensors and destination. These relays insert redundant information by mixing the incoming streams from sensor nodes, hence creating parity check information which is very useful to help decode the transmit information from sensors. The goal is to combine network and channel coding to match network on graph to code on graph, and this is called adaptive network coded cooperation (ANCC). Compared to the previous works in the field, we propose a new transmit protocol based on the use of beamforming technique which is very efficient in terms of throughput and is fully compatible with the physical network coding (PNC) principles. Furthermore, we propose a distributed coding scheme where the relay, the destination, and the sensors are all equipped with repeat accumulate (RA) channel code structures. The relay has a special RA structure to suit the need of the PNC multiple access channel.
Proinflammatory Cytokine Induction of 11β-Hydroxysteroid Dehydrogenase Type 1 (11β-HSD1) in Human Adipocytes Is Mediated by MEK, C/EBPβ, and NF-κB/RelA
Authors: Esteves Cristina L. , Kelly Val , Breton Amandine , Taylor Ashley I. , West Christopher C. , Donadeu Francesc X. , Péault Bruno , Seckl Jonathan R. , Chapman Karen E. 
Published: November 2013

Context:
Levels of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1), which regenerates active glucocorticoids, are selectively elevated in adipose tissue in human obesity and metabolic syndrome, both conditions associated with chronic low-grade inflammation. 11β-HSD1 expression is induced by proinflammatory cytokines in a variety of cell types, including in human adipocytes differentiated in vitro.

Objective:
Our objective was to determine the mechanisms by which proinflammatory cytokines induce 11β-HSD1 in human adipocytes.

Results:
The proinflammatory cytokines IL-1α (10 ng/mL) and TNFα (20 ng/mL) increased 11β-HSD1 mRNA levels in human primary adipocyte fractions and Simpson-Golabi-Behmel syndrome (SGBS) adipocytes (P &lt; .001). Inhibition of the MAPK/ERK kinase (MEK) attenuated CCAAT/enhancer binding protein (C/EBP) β phosphorylation at Thr235 and IL-1α/TNFα induction of 11β-HSD1 (P ≤ .007). The small interfering RNA-mediated knockdown of C/EBPβ and nuclear factor (NF)-κB/RelA or inhibition of NF-κB/RelA also attenuated cytokine induction of 11β-HSD1 (P ≤ .001). Moreover, induction of 11β-HSD1 by IL-1α in SGBS cells was associated with nuclear localization of C/EBPβ and NF-κB/RelA. Chromatin immunoprecipitation experiments showed C/EBPβ and NF-κB/RelA located to the 11β-HSD1 promoter in human adipose tissue. Treatment of adipocyte fractions or SGBS adipocytes with metformin or acetylsalicylic acid, which target C/EBPβ and NF-κB/RelA signaling, attenuated the IL-1α induction of 11β-HSD1 (P ≤ .002).

Conclusions:
Increased proinflammatory signaling in inflamed adipose tissue may mediate elevated 11β-HSD1 expression at this site via MEK, C/EBPβ, and NF-κB/RelA. These molecules/signaling pathways are, therefore, potential targets for drugs, including metformin and acetylsalicylic acid, to prevent/decreased up-regulation of 11β-HSD1 in human obese/metabolic syndrome adipose tissue.
A Novel Deletion of IGF1 in a Patient With Idiopathic Short Stature Provides Insight Into IGF1 Haploinsufficiency
Authors: Batey Lara , Moon Jennifer E. , Yu Yongguo , Wu Bingbing , Hirschhorn Joel N. , Shen Yiping , Dauber Andrew 
Published: November 2013

Context:
Short stature is a common reason for referral to pediatric endocrinology centers. Frequently, the underlying etiology of short stature is unknown, resulting in a diagnosis of idiopathic short stature. Rare genetic defects in the GH/IGF-1 axis have been found to cause short stature.

Objective:
The objective of this study was to identify the genetic etiology of short stature in a patient with Idiopathic Short Stature and to review the clinical presentation of patients with genetic defects in IGF1, and specifically IGF-1 haploinsufficiency.

Design/Setting/Participants:
The index patient was evaluated at an academic medical center, and DNA was obtained from the proband and both parents.

Intervention:
Genome-wide copy number analysis was performed in the proband with confirmatory quantitative PCR in the proband and his parents.

Main Outcome Measure:
We measured novel copy number variants (CNVs) thought to explain the patient's short stature.

Results:
CNV analysis revealed that the proband carried a paternally inherited heterozygous IGF1 gene deletion. His phenotypic features are consistent with those found in previous case reports of IGF-1 deficiency.

Conclusions:
This study, as the first case of a complete heterozygous 1GF1 deletion, provides insight into the effects of true IGF-1 haploinsufficiency. Given the similarities in phenotype between the present proband and those previously described, it is highly likely that his IGF1 deletion is the cause for his short stature. Broadly, this study emphasizes how CNV analysis and other genetic sequencing techniques are evolving as an important tool to identify genetic causes underlying human disease, allowing for improved diagnosis and targeted treatment.
Activation of Drosophila hemocyte motility by the ecdysone hormone
Authors: Sampson Christopher J. , Amin Unum , Couso Juan-Pablo 
Published: November 2013

Summary
Drosophila hemocytes compose the cellular arm of the fly's innate immune system. Plasmatocytes, putative homologues to mammalian macrophages, represent ∼95% of the migratory hemocyte population in circulation and are responsible for the phagocytosis of bacteria and apoptotic tissues that arise during metamorphosis. It is not known as to how hemocytes become activated from a sessile state in response to such infectious and developmental cues, although the hormone ecdysone has been suggested as the signal that shifts hemocyte behaviour from quiescent to migratory at metamorphosis. Here, we corroborate this hypothesis by showing the activation of hemocyte motility by ecdysone. We induce motile behaviour in larval hemocytes by culturing them with 20-hydroxyecdysone ex vivo. Moreover, we also determine that motile cell behaviour requires the ecdysone receptor complex and leads to asymmetrical redistribution of both actin and tubulin cytoskeleton.
The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms
Authors: Jodoin Jeanne N. , Shboul Mohammad , Albrecht Todd R. , Lee Ethan , Wagner Eric J. , Reversade Bruno , Lee Laura A. 
Published: November 2013

Summary
We previously reported that the small nuclear RNA processing complex, Integrator, is required for dynein recruitment to the nuclear envelope at mitotic onset in cultured human cells. We now report an additional role for INT in ciliogenesis. Depletion of INT subunits from cultured human cells results in loss of primary cilia. We provide evidence that the requirements for INT in dynein localization and ciliogenesis are uncoupled: proteins essential for ciliogenesis are not essential for dynein recruitment to the nuclear envelope, while depletion of known regulators of perinuclear dynein has minimal effects on ciliogenesis. Taken together, our data support a model in which INT ensures proper processing of distinct pools of transcripts encoding components that independently promote perinuclear dynein enrichment and ciliogenesis.
The uncertainty of ASCOT and the second-order hesitation of ASCO2.T within the transdisciplinary buffer zone, Round 2
Authors: Ljubec Živa 
Published: November 2013

Abstract
The first round about ‘The myth of ASCOT and its rival ASCO2.T: tech-noetic vs. techno-logic’ exposed the hazard in colliding obsolete disciplinary categories under outdated procedures. The orthodox jurisdiction of Ars Electronica and CERN in Collide@CERN, one of the most prominent ongoing programmes of this kind, does not eliminate the risk of missing the target by operating with categories of artists and scientists. Art is one of those disciplines with a long expired warranty, but with decay on its periphery that is turning into fertile forefront territories. Fresh temporary categories are marking and spreading over these uncharted territories and sensibly interconnecting with peripheries of other disciplines. The ex-artist that is reborn in this peripheral transdisciplinary zone can be provisionally categorized as a polyphibian for its features to be carefully studied. Like amphibians, a polyphibian can coherently transcend from one medium to another, in between and beyond the disciplines. In order to research the implications of such ‘categorical’ mutations, a readymade was submitted to the organization of Collide@CERN under the licence of R. Mutt. Namely, the readymade ASCOTT (Apparatus with Super COnducting Thought Transduction) dubbed also ASCO2.T is needed as the second-order upgrade of the existing plan for ASCOT (Apparatus with Super COnducting Toroids) invented by the scientists for CERN. As is expected from the reputation of R. Mutt’s readymades – this submission was ignored and refused in just the same quiet manner as his most notorious one. But if society would not provide salons for the refused artists (Salon des Refuses) right next to the salons under the scrutiny of academically established artists, there would be no mutations and no evolution in art. Such mutations of the artist into a ‘complex and widely distributed system’ at the dispersing fringes of decomposing art was already predicted by R. Ascott. Polyphibians are the species surviving the ripening metamorphosis of disciplines by taking refuge in the unexplored transdisciplinary buffer zone. This is the only zone where a confrontation of ASCOT technology with tech-noetics of ASCO2.T is possible. The refused unknown demands not a Salon but the Interval of Suspended Judgement. In the sequel to the first round of this debate, the Interval of Suspended Judgement will be investigated.
Hitchhiking of Deleterious Alleles and the Cost of Adaptation in Partially Selfing Species
Authors: Hartfield Matthew , Glémin Sylvain 
Published: November 2013

AbstractSelf-fertilization is generally seen to be disadvantageous in the long term. It increases genetic drift, which subsequently reduces polymorphism and the efficiency of selection, which also challenges adaptation. However, high selfing rates can increase the fixation probability of recessive beneficial mutations, but existing theory has generally not accounted for the effect of linked sites. Here, we analyze a model for the fixation probability of deleterious mutants that hitchhike with selective sweeps in diploid, partially selfing populations. Approximate analytical solutions show that, conditional on the sweep not being lost by drift, higher inbreeding rates increase the fixation probability of the deleterious allele, due to the resulting reduction in polymorphism and effective recombination. When extending the analysis to consider a distribution of deleterious alleles, as well as the average fitness increase after a sweep, we find that beneficial alleles generally need to be more recessive than the previously assumed dominance threshold (h &lt; 1/2) for selfing to be beneficial from one-locus theory. Our results highlight that recombination aiding the efficiency of selection on multiple loci amplifies the fitness benefits of outcrossing over selfing, compared to results obtained from one-locus theory. This effect additionally increases the parameter range under which obligate outcrossing is beneficial over partial selfing.
Energy Consumption of Battery-Operated Screwdriver at Assembling Fixed Threaded Joints / Akumulatora Skrūvgrieža Elektroenerģijas Patēriņa Aprēķins, Veicot Nekustīga Vītņu Savienojuma Salikšanu Atkarībā No Operatora Reakcijas Laika
Authors: Grinevich I. , Mozga N. , Rudzitis J. 
Published: November 2013

Abstract
The electrical energy consumption of battery-operated screwdrivers, which are widely used in many industries, e.g. automotive, heavy, chemical, etc., can be considerably reduced. This would allow increasing the service life of a battery and reducing the time of its charging, thus increasing the productivity and decreasing the prime cost of this battery. It is shown that up to 17.9% of electrical energy could be saved at assembling fixed threaded joints by removing power from the screwdriver immediately after reaching the required tightening torque.
Responsiveness in Rheumatoid Arthritis. A Report from the OMERACT 11 Ultrasound Workshop
Authors: Iagnocco Annamaria , Naredo Esperanza , Wakefield Richard , Bruyn George A.W. , Collado Paz , Jousse-Joulin Sandrine , Finzel Stephanie , Ohrndorf Sarah , Delle Sedie Andrea , Backhaus Marina , Berner-Hammer Hilde , Gandjbakhch Frederique , Kaeley Gurjit , Loeuille Damien , Moller Ingrid , Terslev Lene , Aegerter Philippe , Aydin Sibel , Balint Peter V. , Filippucci Emilio , Mandl Peter , Pineda Carlos , Roth Johannes , Magni-Manzoni Silvia , Tzaribachev Niolay , Schmidt Wolfgang A. , Conaghan Philip G. , D’Agostino Maria-Antonietta 
Published: November 2013

Objective.To summarize the work performed by the Outcome Measures in Rheumatology (OMERACT) Ultrasound (US) Task Force on the validity of different US measures in rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) presented during the OMERACT 11 Workshop.Methods.The Task Force is an international group aiming to iteratively improve the role of US in arthritis clinical trials. Recently a major focus of the group has been the assessment of responsiveness of a person-level US synovitis score in RA: the US Global Synovitis Score (US-GLOSS) combines synovial hypertrophy and power Doppler signal in a composite score detected at joint level. Work has also commenced examining assessment of tenosynovitis in RA and the role of US in JIA.Results.The US-GLOSS was tested in a large RA cohort treated with biologic therapy. It showed early signs of improvement in synovitis starting at Day 7 and increasing to Month 6, and demonstrated sensitivity to change of the proposed grading. Subsequent voting questions concerning the application of the US-GLOSS were endorsed by > 80% of OMERACT delegates. A standardized US scoring system for detecting and grading severity of RA tenosynovitis and tendon damage has been developed, and acceptable reliability data were presented from a series of exercises. A preliminary consensus definition of US synovitis in pediatric arthritis has been developed and requires further testing.Conclusion.At OMERACT 11, consensus was achieved on the application of the US-GLOSS for evaluating synovitis in RA; and work continues on development of RA tenosynovitis scales as well as in JIA synovitis.
Conditions and threshold for magma transfer in the layered upper crust: Insights from experimental models
Authors: Ritter Malte C. , Acocella Valerio , Ruch Joel , Philipp Sonja L. 
Published: November 2013

AbstractMagma transfer, i.e., dike propagation, is partly controlled by Young's modulus (elasticity) contrasts (ratio upper layer to lower layer modulus) in the host rock. Here we try to better constrain the elasticity contrasts controlling the propagation velocity of dikes and their arrest. We simulate dike propagation in layered elastic media with different elasticity contrasts. Salted gelatin and water represent host rock and magma, respectively. For common density ratios between magma and host rock (~1.1), velocity variations are observed and a critical threshold in the elasticity contrast between layers results in the Young's modulus ratio of 2.1 ± 0.6. Naturally occurring elasticity contrasts can be much higher than this experimental threshold, suggesting that dike arrest due to heterogeneous elastic host rock properties is more frequent than expected. Examples of recently deflected or stalled dikes inside volcanoes and the common presence of high‐velocity bodies below volcanoes suggest that better defining elasticity contrasts below volcanoes helps in forecasting eruptions.