bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations between Water Physicochemistry andPrymnesium parvumPresence, Abundance, and Toxicity in West Texas Reservoirs
Authors: VanLandeghem Matthew M. , Farooqi Mukhtar , Southard Greg M. , Patiño Reynaldo 
Published: November 2014

AbstractToxic blooms of golden alga (Prymnesium parvum) have caused substantial ecological and economic harm in freshwater and marine systems throughout the world. In North America, toxic blooms have impacted freshwater systems including large reservoirs. Management of water chemistry is one proposed option for golden alga control in these systems. The main objective of this study was to assess physicochemical characteristics of water that influence golden alga presence, abundance, and toxicity in the Upper Colorado River basin (UCR) in Texas. TheUCRcontains reservoirs that have experienced repeated blooms and other reservoirs where golden alga is present but has not been toxic. We quantified golden alga abundance (hemocytometer counts), ichthyotoxicity (bioassay), and water chemistry (surface grab samples) at three impacted reservoirs on the Colorado River; two reference reservoirs on the Concho River; and three sites at the confluence of these rivers. Sampling occurred monthly from January 2010 to July 2011. Impacted sites were characterized by higher specific conductance, calcium and magnesium hardness, and fluoride than reference and confluence sites. At impacted sites, golden alga abundance and toxicity were positively associated with salinity‐related variables and blooms peaked at ~10°C and generally did not occur above 20°C. Overall, these findings suggest management of land and water use to reduce hardness or salinity could produce unfavorable conditions for golden alga.
Discovered just before extinction? The first endemic ant from the Balearic Islands (Lasius balearicus sp. nov.) is endangered by climate change
Authors: Talavera Gerard , Espadaler Xavier , Vila Roger 
Published: November 2014

AbstractAimWe analyse the taxonomic status, phylogenetic relationships, distribution and age of a newly discovered ant taxon found in the mountaintops of the island of Mallorca (Spain). We also consider the potential impact of short‐term climate change on the survival of this ant and make proposals on its conservation status, risks and management.LocationBalearic Islands (Spain).MethodsWe used morphological, molecular and ecological evidence to assess the specific status of the potential new species. We gathered distribution data to conduct climate‐based distribution modelling of present and future occupancy under several SRES emission scenarios.ResultsThe existence of a new non‐cryptic species of ant (Lasius balearicus Talavera, Espadaler & Vila, sp. nov.) is described from the island of Mallorca. Its distribution was found to be extremely restricted (Serra de Tramuntana) and elevationally constrained to island summits (between 800 and 1400 m a.s.l.). Molecular dating indicated that this species diverged about 1.51 million years ago from its nearest relatives, from which it can be distinguished based on several morphological traits. Ecological niche modelling shows a dramatic reduction of areas with suitable climatic conditions under the different scenarios studied.Main conclusionsLasius balearicus represents the first endemic ant to be described in the Balearic Islands, as well as the first endemic Lasius species in the Mediterranean islands. Distribution modelling predictions, the low intraspecific genetic diversity observed, and the geographical and elevational isolation of the populations indicated a low probability for the survival of the species in the short term, thus making it a potential model to study real‐time climate‐based biodiversity loss. As a consequence, we strongly recommend including L. balearicus in the IUCN Red List of Threatened Species under the category ‘Endangered’. This case illustrates that a fraction of biodiversity remains unexplored even within Europe, arguably the best‐studied region of the planet, and that the available time‐window for us to study and protect it may be in some instances notably narrow.
Present status of clinical deployment of glucokinase activators
Authors: Nakamura Akinobu , Terauchi Yasuo 
Published: November 2014

AbstractGlucokinase is one of four members of the hexokinase family of enzymes. Its expression is limited to the major organs (such as the pancreas, liver, brain and the gastrointestinal tract) that are thought to have an integrated role in glucose sensing. In the liver, phosphorylation of glucose by glucokinase promotes glycogen synthesis, whereas in the β‐cells, it results in insulin release. Studies of glucokinase‐linked genetically‐modified mice and mutations in humans have illustrated the important roles played by glucokinase in whole‐body glucose homeostasis, and suggest that the use of pharmacological agents that augment glucokinase activity could represent a viable treatment strategy in patients with type 2 diabetes. Since 2003, many glucokinase activators (GKAs) have been developed, and their ability to lower the blood glucose has been shown in several animal models of type 2 diabetes. Also, we and others have shown in mouse models that GKAs also have the effect of stimulating the proliferation of β‐cells. However, the results of recent phase II trials have shown that GKAs lose their efficacy within several months of use, and that their use is associated with a high incidence of hypoglycemia; furthermore, patients treated with GKAs frequently developed dyslipidemia. A better understanding of the role of glucokinase in metabolic effects is required to resolve several issues identified in clinical trials.
Postcopulatory inbreeding avoidance in guppies
Authors: Fitzpatrick J. L. , Evans J. P. 
Published: November 2014

AbstractIn many species, the negative fitness effects of inbreeding have facilitated the evolution of a wide range of inbreeding avoidance mechanisms. Although avoidance mechanisms operating prior to mating are well documented, evidence for postcopulatory mechanisms of inbreeding avoidance remain scarce. Here, we examine the potential for paternity biases to favour unrelated males when their sperm compete for fertilizations though postcopulatory inbreeding avoidance mechanisms in the guppy, Poecilia reticulata. To test this possibility, we used a series of artificial inseminations to deliver an equal number of sperm from a related (either full sibling or half sibling) and unrelated male to a female while statistically controlling for differences in sperm quality between rival ejaculates. In this way, we were able to focus exclusively on postcopulatory mechanisms of inbreeding avoidance and account for differences in sperm competitiveness between rival males. Under these carefully controlled conditions, we report a significant bias in paternity towards unrelated males, although this effect was only apparent when the related male was a full sibling. We also show that sperm competition generally favours males with highly viable sperm and thus that some variance in sperm competitiveness can be attributed to difference in sperm quality. Our findings for postcopulatory inbreeding avoidance are consistent with prior work on guppies, revealing that sperm competition success declines linearly with the level of relatedness, but also that such effects are only apparent at relatedness levels of full siblings or higher. These findings reveal that postcopulatory processes alone can facilitate inbreeding avoidance.
Bergmann's Rule rules body size in an ectotherm: heat conservation in a lizard along a 2200‐metre elevational gradient
Authors: Zamora‐Camacho F. J. , Reguera S. , Moreno‐Rueda G. 
Published: November 2014

AbstractBergmann's Rule predicts larger body sizes in colder habitats, increasing organisms' ability to conserve heat. Originally formulated for endotherms, it is controversial whether Bergmann's Rule may be applicable to ectotherms, given that larger ectotherms show diminished capacity for heating up. We predict that Bergmann's Rule will be applicable to ectotherms when the benefits of a higher conservation of heat due to a larger body size overcompensate for decreased capacity to heating up. We test this hypothesis in the lizard Psammodromus algirus, which shows increased body size with elevation in Sierra Nevada (SE Spain). We measured heating and cooling rates of lizards from different elevations (from 300 to 2500 m above sea level) under controlled conditions. We found no significant differences in the heating rate along an elevational gradient. However, the cooling rate diminished with elevation and body size: highland lizards, with larger masses, have a higher thermal inertia for cooling, which allows them to maintain heat for more time and keep a high body temperature despite the lower thermal availability. Consequently, the net gaining of heat increased with elevation and body size. This study highlights that the heat conservation mechanism for explaining Bergmann's Rule works and is applicable to ectotherms, depending on the thermal benefits and costs associated with larger body sizes.
Should Derivatives Be Privileged in Bankruptcy?
Authors: BOLTON PATRICK , OEHMKE MARTIN 
Published: November 2014

ABSTRACTDerivatives enjoy special status in bankruptcy: they are exempt from the automatic stay and effectively senior to virtually all other claims. We propose a corporate finance model to assess the effect of these exemptions on a firm's cost of borrowing and incentives to engage in derivative transactions. While derivatives are value‐enhancing risk management tools, seniority for derivatives can lead to inefficiencies: it transfers credit risk to debtholders, even though this risk is borne more efficiently in the derivative market. Seniority for derivatives is efficient only if it provides sufficient cross‐netting benefits to derivative counterparties that provide hedging services.
Information Acquisition in Rumor‐Based Bank Runs
Authors: HE ZHIGUO , MANELA ASAF 
Published: November 2014

ABSTRACTWe study information acquisition and dynamic withdrawal decisions when a spreading rumor exposes a solvent bank to a run. Uncertainty about the bank's liquidity and potential failure motivates depositors who hear the rumor to acquire additional noisy signals. Depositors with less informative signals may wait before gradually running on the bank, leading to an endogenous aggregate withdrawal speed and bank survival time. Private information acquisition about liquidity can subject solvent‐but‐illiquid banks to runs, and shorten the survival time of failing banks. Public provision of solvency information can mitigate runs by indirectly crowding‐out individual depositors' effort to acquire liquidity information.
Bidirectional‐Compounding Effects of Rumination and Negative Emotion in Predicting Impulsive Behavior: Implications for Emotional Cascades
Authors: Selby Edward A. , Kranzler Amy , Panza Emily , Fehling Kara B. 
Published: November 2014

AbstractInfluenced by chaos theory, the emotional cascade model proposes that rumination and negative emotion may promote each other in a self‐amplifying cycle that increases over time. Accordingly, exponential‐compounding effects may better describe the relationship between rumination and negative emotion when they occur in impulsive persons, and predict impulsive behavior. Forty‐seven community and undergraduate participants who reported frequent engagement in impulsive behaviors monitored their ruminative thoughts and negative emotion multiple times daily for two weeks using digital recording devices. Hypotheses were tested using cross‐lagged mixed model analyses. Findings indicated that rumination predicted subsequent elevations in rumination that lasted over extended periods of time. Rumination and negative emotion predicted increased levels of each other at subsequent assessments, and exponential functions for these associations were supported. Results also supported a synergistic effect between rumination and negative emotion, predicting larger elevations in subsequent rumination and negative emotion than when one variable alone was elevated. Finally, there were synergistic effects of rumination and negative emotion in predicting number of impulsive behaviors subsequently reported. These findings are consistent with the emotional cascade model in suggesting that momentary rumination and negative emotion progressively propagate and magnify each other over time in impulsive people, promoting impulsive behavior.
Diversity, Distribution and Effects on Cassava Cultivars of Cassava Brown Streak Viruses in Malawi
Authors: Mbewe Willard , Kumar P. Lava , Changadeya Wisdom , Ntawuruhunga Pheneas , Legg James 
Published: November 2014

AbstractCassava brown streak disease (CBSD) has emerged as a major threat to cassava (Manihot esculenta) in eastern and southern Africa. CBSD was first reported in Malawi in the 1950s, but little data on the distribution and epidemiology of the disease are available. A diagnostic survey was therefore conducted in Malawi to determine the distribution, incidence and diversity of viruses causing the disease, and to characterize its effects on local cassava cultivars. Diagnostic tests confirmed the presence of cassava brown streak viruses (CBSVs) in 90% of leaf samples from symptomatic plants. Average CBSD foliar severity was 2.5, although this varied significantly between districts. Both Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) (genus Ipomovirus, family Potyviridae) were detected from sampled plants. UCBSV was widespread, whereas CBSV was detected only in the two most northerly districts. The average abundance of the whitefly vector (Bemisia tabaci) was 0.4 per plant, a low value that was partly attributable to the fact that the survey was conducted during the cool part of the year known to be unfavourable for B. tabaci whiteflies. Spearman's correlation analyses showed a positive correlation between CBSD foliar incidence and CBSD severity and between CBSD severity and CBSD stem incidence. Of the 31 cassava varieties encountered, 20–20 was most severely affected, whilst Mtutumusi was completely unaffected. Although data from this study do not indicate a significant CBSD deterioration in Malawi, strengthened management efforts are required to reduce the current impact of the disease.
Molecular Characterization of a Cucumber Bulgarian latent virus Isolate from Iran
Authors: Menzel Wulf , Bananej Kaveh , Winter Stephan 
Published: November 2014

AbstractCucumber Bulgarian latent virus (CBLV) was first reported from cucumber in Bulgaria in 2003 and has been assigned to the genus Tombusvirus. Ten years after the first and only report of CBLV, an isolate from a cucumber sample collected in Iran was characterized. Its complete genomic sequence was determined and analysed. Except for the coat protein, CBLV shows the highest sequence identities to the isolates of other species of the genus Tombusvirus. However, sequence comparison and phylogenetic analyses based on the coat protein (CP) revealed that CBLV is more closely related to the genus Aureusvirus rather than to the isolates of the genus Tombusvirus. The sequence identities to some aureusviruses are above the species demarcation threshold value, demonstrating that CBLV is an unusual tombusvirus species. This suggests that it is necessary to review the CP threshold value for species demarcation in the genus Aureusvirus. In addition, CBLV has an intermediate genome size compared to other tombus‐ and aureusviruses. Several polyclonal antisera raised against different tombus‐ and aureusviruses were used to assess the serological relation to CBLV. The ELISA results indicate that CBLV is not serologically related to any of those tested.
Logarithmic Transformation is Essential for Statistical Analysis of Fungicide EC50 Values
Authors: Liang Hong‐Jie , Li Jin‐Li , Di Ya‐Li , Zhang An‐Sheng , Zhu Fu‐Xing 
Published: November 2014

AbstractHalf maximal (50%) effective concentration (EC50) values are widely used to express fungicide potency and sensitivity of plant pathogens. This study explored the necessity of logarithmic transformation for statistical analysis of EC50 values. The results demonstrated that without logarithmic transformation, none of the five sets of epoxiconazole EC50 data (n = 26–33) against Sclerotinia sclerotiorum fitted a normal distribution. But after logarithmic transformation, four of the five datasets became normally distributed. Of the five sets of pyraclostrobin EC50 data (n = 29–32), only one dataset fitted a normal distribution. After logarithmic transformation, four datasets became normally distributed. Logarithmic transformation transformed the heterogeneity of variance across the five sets of epoxiconazole EC50 data to homogeneity but failed to improve the heterogeneity of variance across the five sets of pyraclostrobin EC50 data. For 150 isolates' EC50 values to epoxiconazole and 153 isolates' EC50 values to pyraclostrobin, the intervals of arithmetic means ± standard deviations (SD) covered 85.3% and 90.2% of data points, respectively, whereas the intervals of geometric means (*) multiplied/divided by the multiplicative SD (S*) covered 69.3% and 70.9% of data points, respectively, which approximated the theoretical value of 68.3%. Distribution normality and homogeneity of variance are prerequisites for analysis of variance (anova) and the two parameters could be improved by logarithmic transformation, therefore, power and efficiency of statistical tests on EC50 data will be greatly enhanced by this kind of transformation.
High‐Throughput Assessment and Genetic Investigation of Vegetative Compatibility in Verticillium dahliae
Authors: Papaioannou Ioannis A. , Typas Milton A. 
Published: November 2014

AbstractClassification of isolates into vegetative compatibility groups (VCGs) using nitrate‐non‐utilizing (nit) mutants has been widely used for the characterization of Verticillium dahliae populations. However, certain methodological limitations prevent its application on a large scale. Furthermore, systematic investigations into the genetics underlying complementation tests between nit mutants of fungal isolates (i.e. heterokaryon formation) are lacking for Verticillium species. In this work, a diverse collection of 27 V. dahliae isolates – including representatives of all VCGs, both mating types, and heterokaryon self‐incompatible isolates – was employed for the development and optimization of (i) a protocol for the rapid generation of nit mutants of V. dahliae isolates using UV‐irradiation and (ii) a reproducible high‐throughput procedure for complementation tests between nit mutants in liquid cultures using 96‐well microplates. The genetic analysis of selected heterokaryons demonstrated that the frequently encountered ‘weak’ cross‐reactions between VCGs and their subgroups can be actually heterokaryotic, implying the absence of strict genetic barriers between VCGs. In conclusion, we provide in this work an optimized method for the high‐throughput VCG assignment of V. dahliae populations and a genetic analysis of heterokaryons that may have serious implications for the interpretation of VCG classification data. These advancements in the available methodology and the genetic background of vegetative compatibility grouping may contribute to a better understanding of the population biology of V. dahliae and possibly other mitosporic fungi.
Melatonin attenuates neutrophil inflammation and mucus secretion in cigarette smoke‐induced chronic obstructive pulmonary diseases via the suppression of Erk‐Sp1 signaling
Authors: Shin In‐Sik , Shin Na‐Rae , Park Ji‐Won , Jeon Chan‐Mi , Hong Ju‐Mi , Kwon Ok‐Kyoung , Kim Joong‐Sun , Lee In‐Chul , Kim Jong‐Choon , Oh Sei‐Ryang , Ahn Kyung‐Seop 
Published: November 2014

AbstractThe incidence of chronic obstructive pulmonary disease (COPD) has substantially increased in recent decade. Cigarette smoke (CS) is the most important risk factor in the development of COPD. In this study, we investigated the effects of melatonin on the development of COPD using a CS and lipopolysaccharide (LPS)‐induced COPD model and cigarette smoke condensate (CSC)‐stimulated NCI‐H292 cells, a human mucoepidermoid carcinoma cell. On day 4, the mice were treated intranasally with LPS. The mice were exposed to CS for 1 hr per day (8 cigarettes per day) from day 1 to day 7. Melatonin (10 or 20 mg/kg) was injected intraperitoneally 1 hr before CS exposure. Melatonin markedly decreased the neutrophil count in the BALF, with reduction in the proinflammatory mediators and MUC5AC. Melatonin inhibited Erk phosphorylation and Sp1 expression induced by CS and LPS treatment. Additionally, melatonin decreased airway inflammation with a reduction in myeloperoxidase expression in lung tissue. In in vitro experiments, melatonin suppressed the elevated expression of proinflammatory mediators induced by CSC treatment. Melatonin reduced Erk phosphorylation and Sp1 expression in CSC‐stimulated H292 cells. In addition, cotreatment of melatonin and Erk inhibitors significantly limited the proinflammatory mediators with greater reductions in Erk phosphorylation and Sp1 expression than that observed in H292 cells treated with Erk inhibitor alone. Taken together, melatonin effectively inhibited the neutrophil airway inflammation induced by CS and LPS treatment, which was closely related to downregulation of Erk phosphorylation. These findings suggest that melatonin has a therapeutic potential for the treatment of COPD.
Hepatitis C virus NS5A drives a PTEN‐PI3K/Akt feedback loop to support cell survival
Authors: Cheng Du , Zhang Leiliang , Yang Guangbo , Zhao Lei , Peng Feng , Tian Yi , Xiao Xinqiang , Chung Raymond T. , Gong Guozhong 
Published: November 2014

AbstractBackground & AimsDecreased levels of phosphatase and tensin homologue (PTEN) are associated with hepatocellular carcinoma (HCC) pathogenesis and poor prognosis in hepatitis C virus (HCV)‐infected HCC patients. The molecular processes governing the reduction in PTEN and outcome of PTEN dysfunction in hepatocytes are poorly understood.MethodsThe levels of proteins and mRNA were assessed by real time PCR and immunoblot. PTEN promoter activity was measured by reporter assay. Signalling pathways were perturbed using siRNAs or pharmacological inhibitors.ResultsHere, we report that HCV down‐regulates PTEN expression at the transcriptional level by decreasing its promoter activity, mRNA transcription, and protein levels. We further identify NS5A protein as a key determinant of PTEN reduction among HCV proteins. NS5A‐mediated down‐regulation of PTEN occurs through a cooperation of reactive oxygen species (ROS)‐dependent Nuclear Factor‐ kappa B (NF‐κB) and ROS‐independent phosphoinositol‐3‐kinase (PI3K) pathways. Moreover, NS5A protects cells against apoptosis. In addition, we found that down‐regulation of PTEN relieves its inhibitory effect on PI3K‐Akt pathway and triggers cumulative activation of Akt. This PTEN‐PI3K/Akt feedback network mediates the suppression of cell apoptosis caused by NS5A.ConclusionsThese data demonstrate that HCV NS5A down‐regulates PTEN expression through a cooperation of ROS‐dependent and ‐independent pathways that subsequently drives a PTEN‐PI3K/Akt feedback loop to support cell survival. Our findings provide new insights suggesting that NS5A contributes to HCV‐related hepatocarcinogenesis.