bluuebunny/crossref_metadata_2025_split huggingface dataset:
Localized Bullous Pemphigoid: Report of a Case with an Immunofluorescence and Electron Microscopical Studies on the Lesional Distribution of 180‐KD Bullous Pemphigoid Antigen, β4 Integrin, and Type VII Collagen
Authors: Kitajima Yasuo , Suzuki Masayuki , Johkura Yoji , Yaoita Hideo 
Published: April 2015

AbstractA 67‐year‐old woman with a left‐sided hemiplegia had localized bullous pemphigoid demonstrating typical clinical lesions on the left pretibial skin and the radial‐side skin of the right forearm. The histology showed a subepidermal blister with extensive hyperkeratosis, hypergranulosis, and acanthosis. Direct immunofluorescence revealed distinct linear deposits of IgG and C3 at the dermo‐epidermal junction in the perilesional skin and in the roof of the blisters, but few deposits in nonlesional skin. Electron microscopy revealed separation in the lamina lucida. Indirect immunofluorescence of type VII collagen showed its localization in the blister floor. The distribution of the 180‐KD bullous pemphigoid antigen (BPA) and β4 integrin, hemidesmosomal transmembrane proteins, were studied in the lesional skin by indirect immunofluorescence. Both 180‐KD BPA and β4 integrin were localized in the blister roof. By immunoelectron microscopy, β4 integrin was detected in small groups on the cell surface facing the blister cavity. Since the epitope of the monoclonal antibody to 180‐KD BPA used here is known to be localized at a distance of 20 to 50 nm from the membrane surface and this epitope retained in the blister roof, it appears that the blister was produced in the deep lamina lucida. The lesions were cleared with topical 0.05% clobetasole propionate ointment.
Langerhans' Cells of Elderly Patients with Decubital Ulcers
Authors: Kohn David , Kohn Sarah 
Published: April 2015

AbstractAn ultrastructural and morphometric study compared Langerhans' cells in sacral epidermis, 8–10 cm from the lesion of patients (mean age 70) with decubital ulcers, with those in the patients' own normal epidermis from the upper leg and with those in the epidermis from the upper leg of normal age‐matched volunteers. The Langerhans' cell section area was significantly lower in patients' control leg epidermis (25.86 ± 2.29 μm2) than in that from normal controls (35.74 ± 3.76 μm2) or that from patients' sacral epidermis near the lesion (41.26 ± 3.45). The number of Langerhans' cell granules was higher in control leg epidermis of patients (10.22 ± 1.26) and was significantly higher in lesioned sacral epidermis (12.94 ± 1.90) than in normal controls (6.97 ± 1.47). In Langerhans' cells in patients' epidermis, formative stages of Langerhans' cell granules were observed. The findings may indicate that Langerhans' cells in patients' epidermis are in an active state.
Vitiligo, Rheumatoid Arthritis and Pernicious Anemia
Authors: Abraham Zeev , Rozenbaum Michael , Glück Zahava , Feuerman Eleasar J. , Lahat Nitza , Kinarty Amalia 
Published: April 2015

AbstractA patient with a 46‐year history of vitiligo who also presented rheumatoid arthritis and pernicious anemia is described. Meticulous physical examination excluded further systemic or cutaneous involvement. The immunological workup revealed a low CD4 cell percentage with T cells mostly composed of CD8 cells, a discrepancy between the high percentage of cumulative CD4 + CD8 cells and the measured CD3 proportions, very low NK cytotoxicity toward K562 cells, and almost negligible responses to PHA, Con A and PWM mitogens. The results point to severe T and NK cell functional defects. The pathogenetic significance of these data is discussed.
Fournier's Gangrene: Report of a Case Associated with Paralytic and Mechanical Ileus throughout the Management of the Gangrene
Authors: Maekawa Yoshihiro , Nojiri Keiko , Nogami Reiko 
Published: April 2015

AbstractA 65‐year‐old Japanese male, who was treated for Fournier's gangrene, developed an enlarged erythema over the right thigh and right lower quadrant. The area was surgically debrided, and he was given antibiotics. However, he complained of abdominal swelling with a metallic bowel sound, pain, and vomiting and was then treated for paralytic ileus. Although his symptoms initially improved, he complained again of the same symptoms and underwent surgery for mechanical ileus occurring at the site of a surgical scar from an appendectomy 43 years earlier.This is a very rare case of Fournier's gangrene which caused mechanical ileus of the small intestine and adherence to a peritoneal scar after paralytic ileus due to inflammation of the abdominal fascia following scrotal gangrene.
Relapsing Syphilis
Authors: Yoon Juho , Haam Ik Byeung , Chung Kee‐Yang , Lee Min Geol , Lee Jung Bock 
Published: April 2015

AbstractAlthough penicillin still remains highly effective for syphilis, concerns have been raised that current regimens may be inadequate for the treatment of early syphilis. We report the failure of benzathine penicillin in the treatment of secondary syphilis. Extensive tracing of sexual contact history failed to identify sources of possible reinfection; therefore, we believe that this case represents a treatment failure and an ensuing relapse of secondary syphilis.
Cytokine Production of Peripheral Blood Mononuclear Cells in a Dermatophytosis Patient in Response to Stimulation with Trichophytin
Authors: Koga Tetsuya , Ishizaki Hiroshi , Matsumoto Tadahiko , Hori Yoshiaki 
Published: April 2015

AbstractInterferon‐γ (IFN‐γ), interleukin 2 (IL‐2) and granulocyte/macrophage colony‐stimulating factor (GM‐CSF) were detected in the culture supernatant after 72 hours incubation with trichophytin in the peripheral blood mononuclear cells (PBMC) obtained from a patient who had a dermatophyte infection. These findings indicate that this patient has peripheral T‐lymphocytes that produced IFN‐γ, IL‐2 and GM‐CSF, which may play roles in the development of delayed‐type hypersensitivity (DTH) reaction in the skin.
Blister Formation over a Soft Fibroma of the Nipple
Authors: Higaki Yuko , Yoshinaga Yoko , Kawashima Makoto 
Published: April 2015

AbstractWe report a case of soft fibroma on the nipple which was accompanied by a blister. Histologically, a subepidermal blister overlying the tumor and the degeneration of the lower part of the epidermis were observed. The causes of blister formation are briefly discussed.
Augmentation of In vitro Cytolytic Activity of LAK Cells with Heated ATL‐Derived Cell Lines
Authors: Nakayama Juichiro , Toshitani Akito , Hattori Toshio , Moroi Yoichi , Hori Yoshiaki 
Published: April 2015

AbstractThree adult T‐cell leukemia/lymphoma‐derived cell lines, MT‐2, MJ, and HUT102, were investigated to determine how they responded to hyperthermia, lymphokine‐activated killer (LAK) cells, or a combination of both in vitro. All three cell lines showed a similar sensitivity to LAK cells, but revealed varying degrees of sensitivity to hyperthermia (MT‐2<MJ<HUT102) by 51Cr release assay. Hyperthermia did not cause immediate cell death as determined by the trypan blue exclusion test, but did cause substantial decreases in the numbers of heated cells within 2 days. The density of the cells began to increase thereafter, which was consistent with the results of the experiments labeling the cells with 3H‐TdR after hyperthermia. When the cells were heated at 39–43°C for 1–3 hr and then interacted with various LAK cell/ATL cell (E/T) ratios at 37°C for 4 hr, total cytolysis of the cells increased in a synergistic and/or additive manner over that of the cells without hyperthermia. Prolonged incubation of the cells at high temperature did not necessarily cause a large increase in the interaction of LAK cells after hyperthermia. This augmentation of cytolysis by LAK cells after hyperthermia was not seen in normal peripheral lymphocytes. These results suggest that the combination therapy of hyperthermia and LAK cells may be more specific, useful, and effective for treating malignant lymphoma.
Subungual Pigmented Nevus: Evaluation of DNA Ploidy in Six Cases
Authors: Asahina Akihiko , Chi Hyung‐In , Otsuka Fujio 
Published: April 2015

AbstractThe discrimination between subungual pigmented nevus and subungual melanoma in situ is still a clinical problem. We measured DNA ploidy in six cases of subungual melanotic lesions which exhibited the features of subungual pigmented nevus or lentigo simplex histologically. Five cases presented a diploid pattern with or without a slight increase of hyperdiploid cells. One case presented a polyploid pattern; it also exhibited histologically abnormal melanocytes with large nuclei and pigment‐filled elongated dendrites. The DNA ploidy pattern and histologic features suggest that the lesion of this latter case contains abnormal melanocytes which probably have the potential to undergo a malignant transformation into a subungual melanoma. DNA ploidy analysis, therefore, is likely to provide information for evaluating the biologic behavior of subungual melanotic lesions.
A Case of Verrucous Carcinoma Associated with Human Papillomavirus Type 16 DNA
Authors: Nishikawa Takeshi , Kobayashi Hitoshi , Shindoh Masanobu , Yamashita Toshiharu , Fujinaga Kei , Ohkawara Akira 
Published: April 2015

AbstractWe report here a case of verrucous carcinoma which occurred on the penis of a 75‐year‐old male. The nodule was first noted six months earlier and was whitish, cauliflower‐like, and 17 × 19 mm in size. The histopathological examination revealed hypertrophic epidermal proliferation with pale staining keratinocytes, extending into the deep dermis. Partial penectomy and inguinal lymph node dissection were done. No lymph node metastasis was recognized. DNA was isolated from the frozen tumor tissue and examined for the presence of human papillomavirus (HPV) 16, 18, and 33 DNA by the polymerase chain reaction (PCR), using common and specific primers. A 140 base pair (bp) band was amplified and finally determined to be the HPV16 sequence by dot‐blot hybridization.
Linear Porokeratosis
Authors: Taniguchi Yoshiki , Yuasa Tetsuya , Shimizu Masayuki 
Published: April 2015

AbstractA case of linear porokeratosis in a 14‐year‐old girl was reported. The lesions started from the right forearm and extended to the right breast. Histological examinations showed cornoid lamella in the horny layer and dyskeratotic cells in the spinous layer beneath the cornoid lamella. Linear distributions of the lesions resembled the lines of Blaschko.
The Effect of Cimetidine on Verruca Plana Juvenilis: Clinical Trials in Six Patients
Authors: Choi Yong Seop , Hann Seung Kyung , Park Yoon‐Kee 
Published: April 2015

AbstractCimetidine is an H2‐receptor antagonist which is mainly used to treat gastrointestinal diseases. Recently, many authors have suggested that cimetidine has the ability to reverse acquired tolerance to dinitrochlorobenzene (DNCB).We have used cimetidine in six patients with verruca plana who showed no therapeutic response to DNCB. Four of them responded and showed complete disappearance of the verruca plana. The mean duration of oral cimetidine administration (1.2 g/day) was 4 weeks until the disappearance. Therefore, we suggest that oral cimetidine can be used as an effective and safe adjunctive modality for the treatment of verruca plana.
The Relationship of Sun Protection Factor to Minimal Erythema Dose, Japanese Skin Type, and Skin Color
Authors: Kawada Akira , Noda Toshiaki , Hiruma Masataro , Ishibashi Akira , Arai Seiichi 
Published: April 2015

AbstractThe relationship of sun protection factor (SPF) to minimal erythema dose (MED), Japanese skin type, and skin color was investigated on the unexposed backs of 48 healthy subjects. SPF of a UVB‐sunscreen was determined using sun lamps as a light source. A significant correlation was found between SPF and MED; subjects with lower MED showed higher SPF. The average SPF decreased with increasing skin type. There was no significant correlation between SPF and skin color (L*a*b* color system).
The Relationship among Minimal Erythema Dose, Minimal Delayed Tanning Dose, and Skin Color
Authors: Noda Toshiaki , Kawada Akira , Hiruma Masataro , Ishibashi Akira , Arai Seiichi 
Published: April 2015

AbstractThe relationship among minimal erythema dose (MED), minimal delayed tanning dose (MDTD), and skin color was examined in 16 healthy volunteers using three different spectra. The subjects were exposed to UVB, UVA+B, and UV+Visible light (UV+Visible) with a xenon arc solar simulator as a light source. The MEDs for UVB and UVA+B were less than the MDTDs, whereas the MED for UV+Visible was higher than the MDTD. There was no significant correlation between the MED and the MDTD for UVB or UVA+B. The MED for UV+Visible was significantly correlated to the MDTD (p<0.01). Skin color significantly correlated with MEDs for UVB and UVA+B (p<0.01), but not for UV+Visible. There was no significant correlation between skin color and the MDTD for any spectra. From these results, it is suggested that the relationship between erythemal and melanogenic responses is dependent on spectral bands of the light source and that skin color is a predictor of UV‐induced erythema.
Experimental Murine Protoporphyria Induced by Griseofulvin (GF): The Relationship between Hepatic Porphyrin Levels and Liver Function Test Values in Mice Treated with GF
Authors: Tanaka Keiichi , Ohgami Taro , Nonaka Shigeo 
Published: April 2015

AbstractTo investigate the hepatic abnormalities accompanying experimental protoporphyria due to griseofulvin (GF), liver function test values and porphyrin levels in mice were assayed at days 2, 4, 8, and 16 after starting the administration of 0.5% GF feed. Furthermore, in an attempt to elucidate the harmful effects of GF on liver functions, the above mentioned assay was also performed after the feed was discontinued in mice given 0.5% GF feed for 16 days.The hepatic protoporphyrin (PP) level had already risen by day 2, but the erythrocytic PP level was within normal limits at that time. Hepatic PP levels increased gradually, followed by an increase in erythrocytic PP levels. The variation in liver function test values roughly paralleled the porphyrin levels. Over the time span of the response to GF, the variations in the serum glutamate oxaloacetate transaminase (S‐GOT) levels, serum glutamate pyruvate transaminase (S‐GPT) levels, and leucine amino peptidase (LAP) levels resembled those in hepatic PP. On the other hand, the changes in alkaline phosphatase (ALP) levels paralleled those of the erythrocytic PP levels.Erythrocytic and fecal protoporphyrin levels decreased to the normal level one month after the discontinuation of GF administration, but the hepatic protoporphyrin level still was 53.6 times higher than the normal level two months after switching to normal feed. The values of liver function tests had returned to within the normal range after one month.By the fourth day after the administration of GF, a brown pigmented material could be observed around the hepatocytes and the Glisson sheath; the amount of this material increased day by day. Its amount gradually decreased after cessation of GF.These results suggested that the hepatic abnormalities were not induced by the deposition of PP in the liver, but by the direct toxicity of GF. This means that GF‐induced protoporphyria mice are not suitable for investigating porphyric damage to the liver because of the strong hepatic intoxication by GF. However, they may be useful for studies of the removal of deposits of porphyrin granules from the liver.
A Case of Acrogeria
Authors: Matsumura Yasuhiro , Hamanaka Hiroshi , Okuwa Takashi 
Published: April 2015

AbstractWe report a 30‐year‐old Japanese male with acrogeria. He was in good health except for the prominent skin atrophy over the hands and feet. In the microscopic view of an atrophic skin biopsy specimen, elastic fibers were clumped and fragmented, especially in the upper dermis. Reviewing fourteen previously reported Japanese cases of acrogeria, the decrease and degeneration of elastic fibers were the prominent characteristic, as was the atrophy of subcutaneous fat.
Isolated Lichen Planus of the Nails Treated with Etretinate
Authors: Kato Naoko , Ueno Hiroo 
Published: April 2015

AbstractA 46‐year‐old Japanese man with isolated lichen planus of the nails is reported. The affected nails were all of the fingernails and the third and fourth toenails of both feet. Histologically, the ventral part of the proximal nail folds showed mild band‐like cell infiltration below the epidermis, and the nail beds (ventral matrix) showed hypergranulosis and epidermal‐dermal interphase activity with liquefaction degeneration of basal cells and a band‐like cell infiltration consisting of lymphoid cells and histiocytes. Systemic use of etretinate in combination with application of steroid lotion was evaluated as very effective. Although the use of retinoids for lichen planus of the nails remains only a suggested method of treatment, a positive trial should be attempted, since this disorder sometimes results in severe nail damage.
1α,25‐Dihydroxy Vitamin D3 Modulation of HLA‐DR mRNA Induced by Gamma‐Interferon in Cultured Epithelial Tumor Cell Lines
Authors: Tone Takeshi , Eto Hikaru , Katou Takako , Otani Fumio , Nishiyama Shigeo 
Published: April 2015

AbstractUsing three cultured epithelial tumor cell lines, we investigated and analyzed the effects of gamma‐interferon (γ‐IFN) and 1α,25‐dihydroxy vitamin D3 (1,25‐(OH)2D3) on the levels of HLA‐DR (α) mRNA and HLA‐DR (β) mRNA by Northern blot analysis.After treatment with γ‐IFN alone, the levels of the mRNA increased. Treatment with both γ‐IFN and 1,25‐(OH)2D3 at the same time resulted in a significant decrease in the levels of mRNA in K‐TL‐1, IK‐TL‐2, and M‐TL cells as compared to those induced by γ‐IFN alone.
Analysis of the Interleukin 6 Receptor on Normal Human Keratinocytes by Digital Imaging Fluorescence Microscopy
Authors: Kobayashi Teruaki , Hashimoto Koji , Matsumoto Kunio , Yoshizaki Kazuyuki , Yoshikawa Kunihiko 
Published: April 2015

AbstractThe interleukin 6 receptor (IL 6 receptor) on normal human keratinocytes was analyzed by digital imaging fluorescence microscopy (DIFM). Non‐fixed keratinocytes were incubated with monoclonal anti‐IL 6 receptor antibody (MT18). The fluorescence intensity was quantitatively determined by an interactive laser cytometer (ACAS 470). IL 6 receptor‐positive cells showed high density staining with the specific antibody in the center of the elevated cell surface. Therefore, DIFM appears to be an effective method for study of the distribution of IL 6 receptors at the single living cell level.
Successful Treatment of Adult T‐cell Leukemia/Lymphoma with MACOP‐B, M‐FEPA and VEPP‐B Combination Chemotherapy
Authors: Nagatani Tetsuo , Miyazawa Megumi , Matsuzaki Toshiko , Iemoto Gaijiro , Kim Shu‐taku , Baba Naoko , Miyakawa Kanata , Miyamoto Hideaki , Nakajima Hiroshi , Hirai Yoshio 
Published: April 2015

AbstractA 45‐year‐old man was referred to our department in March of 1989. Physical examination showed erythroderma, palmo‐plantar hyperkeratosis, generalized lymphadenopathy, hepatosplenomegaly, and leukemic manifestation. The lymphocyte count in the peripheral blood before treatment was 1.7 × 104 cells/mm3. Atypical lymphocytes such as flower cells and lobulated cells were seen in the peripheral blood. A sample excised from a lymph node showed immunoblastic, pleomorphic T cells by a modified classification scheme of the Working Formulation. A high level of serum LDH was detected (2.1 times the upper normal limit). Anti HTLV‐1 antibody was also detected in the serum. The atypical lymphocytes were positive for CD3, CD4, CD5, CD7 and HLA‐DR, and negative for CD8. Thus, the clinical, pathologic and immunologic features were those of typical acute‐type ATL.The patient was treated with VEPA‐M for three months starting in March of 1989. Because of poor response, the patient was then treated with MACOP‐B, M‐FEPA, and VEPP‐B for about one year from June of 1989 and has been free of disease up to the time of writing, March of 1993.
A Combination of Speckled Lentiginous Nevus with Patch‐type Blue Nevus
Authors: Misago Noriyuki , Narisawa Yutaka , Kohda Hiromu 
Published: April 2015

AbstractA peculiar case of “nevus on nevus” was reported. A 67‐year‐old man had had a pigmented lesion in the left hypochondrial area since birth. The clinicopathologic findings of the pigmented lesion revealed a combination of speckled lentiginous nevus and patch‐type blue nevus. This case of “nevus on nevus” is not described under the term of combined nevus as is current in the literature; it was considered to be a subtype of the type II atypical blue nevus described by Kawamura.
Joint Pains with Psoriasis and Long‐term Systemic Etretinate Therapy: A Case Report and Summary of 12 Cases
Authors: Ishida Yoshitsugu , Ozawa Akira , Ohkido Muneo , Imai Nozomu 
Published: April 2015

AbstractWe report a case of psoriasis with joint pain related to long‐term etretinate therapy. In addition, our experiences with twelve additional patients with psoriasis who also received etretinate therapy are discussed.
Increased Incidence of Antismooth Muscle Antibody in Korean Vitiligo Patients
Authors: Hann Seung Kyung , Im Sungbin , Kim Hyung Il , Kim Hyon‐Suk , Lee Yung Jae , Park Yoon‐Kee 
Published: April 2015

AbstractTo evaluate the incidence of autoimmune disorders and organ‐specific autoantibodies in Korean vitiligo patients, antibodies to nuclear, mitochondrial, smooth muscle, gastric parietal cell, thyroglobulin, and microsomal antigens were screened in 226 vitiligo patients and 120 controls. Of the 226 vitiligo patients, three (1.3%) had thyrotoxicosis and two (0.8%) had diabetes mellitus. The vitiligo patients had an increased incidence of antinuclear (12.4%), antimicrosomal (7.1%), and antismooth muscle antibodies (25.7%). The increased incidence of antismooth muscle antibody was correlated with early onset (less than 15 years), a positive family history of vitiligo, and long duration of vitiligo. These results support an autoimmune origin of vitiligo and suggest that the high incidence of antismooth muscle antibody is a distinctive feature of laboratory findings in Korean vitiligo patients.
A Case of a Giant Schwannoma on the Extremities
Authors: Hagiwara Keisuke , Higa Tadashi , Miyazato Hajime , Nonaka Shigeo 
Published: April 2015

AbstractA 52‐year‐old Japanese female with a human head sized lump on the medial femoral region of her right thigh is described. The histopathological diagnosis after the excision of the tumor was an ordinary type of schwannoma composed of two types of tissue, Antoni types A and B. The tumor was 20 × 15 × 12 cm in size and 2250 g in weight; this is probably the biggest of all the reported cases of schwannoma on the extremities in Japan.
Dermatomyositis, Carcinoma of Colon and Meningioma in the Same Patient
Authors: Glück Zahava , Kutsherowsky Michael , Abraham Zeev , Galper Izequiel 
Published: April 2015

AbstractDermatomyositis and carcinoma of colon were diagnosed in a 66‐year‐old woman. Meticulous physical examination excluded further systemic or cutaneous involvement. The musculocutaneous disorders responded well to daily oral corticosteroid, and the malignant tumor was totally removed surgically. After a seven‐year follow‐up of actual dermatomyositis controlled by maintenance doses of prednisone ranging from 5 to 15 mg daily, the patient developed a meningioma. Current concepts and data regarding various aspects of the combination between dermatomyositis and tumors are discussed. To our knowledge, this is the first reported case of meningioma associated with dermatomyositis.