bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Fluorescence Nanosensor for Glycoproteins with Activity Based on the Molecularly Imprinted Spatial Structure of the Target and Boronate Affinity
Authors: Zhang Wei , Liu Wei , Li Ping , Xiao Haibin , Wang Hui , Tang Bo 
Published: September 2014

AbstractGlycoproteins are closely associated with the occurrence of diverse diseases, and they have been used as biomarkers and therapeutic targets in clinical diagnostics. Currently, mass spectrometry has proven to be a powerful tool for glycoprotein analysis, but it is almost impossible to directly identify glycoproteins without the preparation and pretreatment of samples. Furthermore, biological samples, especially proteins, are damaged by this process. Herein, we describe a novel fluorescence nanosensor based on a molecularly imprinted spatial structure and boronate affinity that is well‐suited for monitoring glycoproteins selectively. Results showed that the recognition performance of the nanosensor for glycoproteins was regulated by controlling the pH value and temperature. Moreover, the nanosensor was successfully applied to the detection of HRP in biological fluids. This study provides a facile and efficient fluorescence tool for glycoprotein detection in clinical diagnostics.
Kinetic, Spectroscopic, and Theoretical Assessment of Associative and Dissociative Methanol Dehydration Routes in Zeolites
Authors: Jones Andrew J. , Iglesia Enrique 
Published: September 2014

AbstractMechanistic interpretations of rates and in situ IR spectra combined with density functionals that account for van der Waals interactions of intermediates and transition states within confining voids show that associative routes mediate the formation of dimethyl ether from methanol on zeolitic acids at the temperatures and pressures of practical dehydration catalysis. Methoxy‐mediated dissociative routes become prevalent at higher temperatures and lower pressures, because they involve smaller transition states with higher enthalpy, but also higher entropy, than those in associative routes. These enthalpy–entropy trade‐offs merely reflect the intervening role of temperature in activation free energies and the prevalence of more complex transition states at low temperatures and high pressures. This work provides a foundation for further inquiry into the contributions of H‐bonded methanol and methoxy species in homologation and hydrocarbon synthesis reactions from methanol.
Large‐Scale Fabrication of Pseudocapacitive Glass Windows that Combine Electrochromism and Energy Storage
Authors: Yang Peihua , Sun Peng , Chai Zhisheng , Huang Langhuan , Cai Xiang , Tan Shaozao , Song Jinhui , Mai Wenjie 
Published: September 2014

AbstractMultifunctional glass windows that combine energy storage and electrochromism have been obtained by facile thermal evaporation and electrodeposition methods. For example, WO3 films that had been deposited on fluorine‐doped tin oxide (FTO) glass exhibited a high specific capacitance of 639.8 F g−1. Their color changed from transparent to deep blue with an abrupt decrease in optical transmittance from 91.3 % to 15.1 % at a wavelength of 633 nm when a voltage of −0.6 V (vs. Ag/AgCl) was applied, demonstrating its excellent energy‐storage and electrochromism properties. As a second example, a polyaniline‐based pseudocapacitive glass was also developed, and its color can change from green to blue. A large‐scale pseudocapacitive WO3‐based glass window (15×15 cm2) was fabricated as a prototype. Such smart pseudocapacitive glass windows show great potential in functioning as electrochromic windows and concurrently powering electronic devices, such as mobile phones or laptops.
Bimetallic Cyclometalated Iridium(III) Diastereomers with Non‐Innocent Bridging Ligands for High‐Efficiency Phosphorescent OLEDs
Authors: Zheng Yonghao , Batsanov Andrei S. , Fox Mark A. , Al‐Attar Hameed A. , Abdullah Khalid , Jankus Vygintas , Bryce Martin R. , Monkman Andrew P. 
Published: September 2014

AbstractTwo phosphorescent dinuclear iridium(III) diastereomers (ΛΔ/ΔΛ) and (ΛΛ/ΔΔ) are readily separated by making use of their different solubilities in hot hexane. The bridging diarylhydrazide ligand plays an important role in the electrochemistry and photophysics of the complexes. Organic light‐emitting devices (OLEDs) that use these complexes as the green‐emissive dopants in solution‐processable single‐active‐layer architectures feature electroluminescence efficiencies that are remarkably high for dinuclear metal complexes, achieving maximum values of 37 cd A−1, 14 lm W−1, and 11 % external quantum efficiency.
Iodide‐Induced Shuttling of a Halogen‐ and Hydrogen‐Bonding Two‐Station Rotaxane
Authors: Caballero Antonio , Swan Laura , Zapata Fabiola , Beer Paul D. 
Published: September 2014

AbstractThe first example of utilizing halogen‐bonding anion recognition to facilitate molecular motion in an interlocked structure is described. A halogen‐bonding and hydrogen‐bonding bistable rotaxane is prepared and demonstrated to undergo shuttling of the macrocycle component from the hydrogen‐bonding station to the halogen‐bonding station upon iodide recognition. In contrast, chloride‐anion binding reinforces the macrocycle to reside at the hydrogen‐bonding station.
Sulfonamide‐Promoted Palladium(II)‐Catalyzed Alkylation of Unactivated Methylene C(sp3)H Bonds with Alkyl Iodides
Authors: Chen Kai , Shi Bing‐Feng 
Published: September 2014

AbstractThe alkylation of unactivated β‐methylene C(sp3)H bonds of α‐amino acid substrates with a broad range of alkyl iodides using Pd(OAc)2 as the catalyst is described. The addition of NaOCN and 4‐Cl‐C6H4SO2NH2 was found to be crucial for the success of this transformation. The reaction is compatible with a diverse array of functional groups and proceeds with high diastereoselectivity. Furthermore, various β,β‐hetero‐dialkyl‐ and β‐alkyl‐β‐aryl‐α‐amino acids were prepared by sequential C(sp3)H functionalization of an alanine‐derived substrate, thus providing a versatile strategy for the stereoselective synthesis of unnatural β‐disubstituted α‐amino acids.
Intercrossed Carbon Nanorings with Pure Surface States as Low‐Cost and Environment‐Friendly Phosphors for White‐Light‐Emitting Diodes
Authors: Li Xiaoming , Liu Yanli , Song Xiufeng , Wang Hao , Gu Haoshuang , Zeng Haibo 
Published: September 2014

AbstractAs an important energy‐saving technique, white‐light‐emitting diodes (W‐LEDs) have been seeking for low‐cost and environment‐friendly substitutes for rare‐earth‐based expensive phosphors or Pd2+/Cd2+‐based toxic quantum dots (QDs). In this work, precursors and chemical processes were elaborately designed to synthesize intercrossed carbon nanorings (IC‐CNRs) with relatively pure hydroxy surface states for the first time, which enable them to overcome the aggregation‐induced quenching (AIQ) effect, and to emit stable yellow‐orange luminescence in both colloidal and solid states. As a direct benefit of such scarce solid luminescence from carbon nanomaterials, W‐LEDs with color coordinate at (0.28, 0.27), which is close to pure white light (0.33, 0.33), were achieved through using these low‐temperature‐synthesized and toxic ion‐free IC‐CNRs as solid phosphors on blue LED chips. This work demonstrates that the design of surface states plays a crucial role in exploring new functions of fluorescent carbon nanomaterials.
Origin and Location of Electrons and Protons during the Formation of Intermetalloid Clusters [Sm@Ga3−xH3−2xBi10+x]3− (x=0, 1)
Authors: Weinert Bastian , Müller Fabian , Harms Klaus , Clérac Rodolphe , Dehnen Stefanie 
Published: September 2014

AbstractReaction of [GaBi3]2− with [Sm(C5Me4H)3] yielded the first protonated ternary intermetalloid clusters [Sm@Ga3−xH3−2xBi10+x]3− (1; x=0,1). The presence of the GaH bonds and the transfer of electrons and protons during the formation of 1 were elucidated by a combination of experimental and quantum chemical methods, thereby rationalizing the role of the solvent ethane‐1,2‐diamine as a Brønsted acid. As an organic by‐product, we observed the previously unknown octamethylfulvene (2) upon CC coupling of (C5Me4H)−.
Copper‐Catalyzed Coupling of Triaryl‐ and Trialkylindium Reagents with Aryl Iodides and Bromides through Consecutive Transmetalations
Authors: Thapa Surendra , Gurung Santosh K. , Dickie Diane A. , Giri Ramesh 
Published: September 2014

AbstractAn efficient copper(I)‐catalyzed coupling of triaryl and trialkylindium reagents with aryl iodides and bromides is reported. The reaction proceeds at low catalyst loadings (2 mol %) and generally only requires 0.33 equivalents of the triorganoindium reagent with respect to the aryl halide as all three organic nucleophilic moieties of the reagent are transferred to the products through consecutive transmetalations. The reaction tolerates a variety of functional groups and sterically hindered substrates. Furthermore, preliminary mechanistic studies that entailed the synthesis and characterization of potential reaction intermediates offered a glimpse of the elementary steps that constitute the catalytic cycle.
Cationic polystyrene spheres for removal of anionic contaminants in white water of papermaking
Authors: Xiao He , He Beihai , Qian Liying , Li Junrong 
Published: September 2014

ABSTRACTIn a paper mill's water circuit, normal runnability of the paper machine is often disturbed due to the buildup of dissolved and colloidal substances (DCS). Contaminant adsorption on a solid adsorbent in a fluidized bed reactor has been applied for white water treatment. In the present study, polystyrene (PS) spheres were firstly acylated by acryloyl chloride, then poly(methacryloxyethyltrimethyl ammonium chloride) (PMAC) was grafted onto the acylated PS spheres, induced by surface‐initiated free‐radical polymerization. Fourier transform infrared spectroscopy, thermal gravimetric analysis, and scanning electron microscopy were used to characterize the changes of PS spheres in chemical structures and surface morphology. The results showed that acylated PS spheres could be easily grafted with PMAC in 53.83% weight increase. Polyelectrolyte loading of PS spheres reached up to 1.72 mmol/g. The resulted cationic modified PS spheres could effectively decrease the cationic demand in white water and it still maintained high performance after operating repeatedly for 10 batches. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 10.1002/app.41379.
Study on the preparation of PU/HBP‐NH2 blend film and its properties
Authors: Longfang Ren , Na Wang , Xuechuan Wang 
Published: September 2014

ABSTRACTThe blend films of polyurethane (PU) and amino‐terminal hyperbranched polymers (HBP‐NH2) were prepared successfully by mixing HBP‐NH2 solution and PU. The rate of moisture absorption and mechanical properties were determined. The results showed that the rates of moisture absorption and vapor permeability were improved from 0.34% to 7.51% and from 161 gm−2 d−1 to 879 gm−2 d−1, respectively. The addition of HBP‐NH2 is helpful to improve the hygiene properties of PU films. Then, the structures of the blend films were characterized by IR, XRD, TG, and SEM at the same time. The results indicated that HBP‐NH2 and PU had hydrogen‐bond interaction and a certain phase separation. The blend films had good heat stability. © 2014 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41383.
An Amphiphilic Selenide Catalyst Behaves Like a Hybrid Mimic of Protein Disulfide Isomerase and Glutathione Peroxidase 7
Authors: Arai Kenta , Moriai Kenji , Ogawa Akinobu , Iwaoka Michio 
Published: September 2014

AbstractProtein disulfide isomerase (PDI) and glutathione peroxidase 7 (GPx7) cooperatively promote the oxidative folding of disulfide (SS)‐containing proteins in endoplasmic reticulum by recognizing the nascent proteins to convert them into the native folds by means of SS formation and SS isomerization and by catalyzing reoxidation of reduced PDI with H2O2, respectively. In this study, new amphiphilic selenides with a long‐chain alkyl group were designed as hybrid mimics of PDI and GPx7 and were applied to the refolding of reduced hen egg‐white lysozyme (HEL‐R). Competitive SS formation at pH 4 using HEL‐R and glutathione (GSH) in the presence of the selenide catalyst and H2O2 showed that the amphiphilic selenides can preferentially catalyze SS formation of HEL‐R, probably on account of hydrophobic interactions between the protein and the catalyst. In contrast, simple water‐soluble selenides did not exhibit such behavior. In addition, when the pH of the solution was adjusted to 8.5 after the SS formation, surviving GSH promoted the SS isomerization of misfolded HEL to recover the native SS linkages. Thus, the amphiphilic selenides designed here could mimic the function of the PDI‐GPx7 system. The combination of a water‐soluble selenide and a long‐chain alkyl group would be a useful motif in designing medicines for both protein misfolding diseases and antioxidant therapy.
Large Magnetocaloric Effect in a Dense and Stable Inorganic–Organic Hybrid Cobridged by In Situ Generated Sulfate and Oxalate
Authors: Han Song‐De , Miao Xiao‐Hong , Liu Sui‐Jun , Bu Xian‐He 
Published: September 2014

AbstractA dense and stable inorganic‐organic hybrid with distorted cubic [Gd4O4] units as building blocks bridged by in situ generated sulfate and oxalate was synthesized. Magnetic measurements indicate that the title complex features a −ΔSmmax=51.49 J kg−1 K−1, which is among the highest values reported so far.
4‐Channel Sliding Mode Control of Teleoperation Systems with Disturbances
Authors: Liu Xia , Dong Xiucheng 
Published: September 2014

AbstractSliding mode control can effectively account for the disturbances of a system. Among the different teleoperation architectures, 4‐channel architecture is the most successful for fulfilling transparency. In this paper, two sliding mode controllers are designed for nonlinear master and slave with external disturbances and are incorporated into a 4‐channel structure to achieve transparency. To this end, each of the controllers consists of a sliding mode position feedback law, a force feedback law, and two supplementary terms regarding gravity and contact force compensation. Stability and transparency of the overall system is studied via a Lyapunov function analysis. Simulations compared with the conventional adaptive control on teleoperation systems demonstrate the effectiveness of the proposed scheme.
Comparative proteomic analysis of antagonistic Bacillus amyloliquefaciens Q‐426 cultivated under different pH conditions
Authors: Zhao Jing , Zhao Pengchao , Quan Chunshan , Jin Liming , Zheng Wei , Fan Shengdi 
Published: September 2014

AbstractBacillus amyloliquefaciens Q‐426 produces lipopeptide compounds with antifungal activities. Initial pH value has a significant influence on the production of lipopeptide compounds. The correlation between pH and intrinsic mechanism of lipopeptide production was rarely discussed. In this research, comparative proteomics, using two‐dimensional gel electrophoresis and mass spectrometry, was applied to identify B. amyloliquefaciens Q‐426 intracellular proteins differentially expressed under initial pH 5.0 and 7.3. A total of 24 differential spots (eight downregulated and 16 upregulated) under pH 5.0 were identified. Certain proteins were involved in the regulation of bacillomycin and fengycin production by B. amyloliquefaciens Q‐426. These proteins include four induced proteins related to stress response: Thiamine pyrophosphate‐dependent acetoin dehydrogenase, butanediol dehydrogenase, two ABC‐type oligopeptide transport system proteins, and two‐component response regulator DegU and chorismate mutase PheB. These results indicate intrinsic differences of antagonistic B. amyloliquefaciens Q‐426 under different pH conditions.
Simultaneous determination of desloratadine and montelukast sodium using second‐derivative synchronous fluorescence spectrometry enhanced by an organized medium with applications to tablets and human plasma
Authors: Ibrahim F. A. , El‐Enany N. , El‐Shaheny R. N. , Mikhail I. E. 
Published: September 2014

AbstractA rapid, simple, and sensitive second‐derivative synchronous fluorimetric method has been developed and validated for the simultaneous analysis of a binary mixture of desloratadine (DSL) and montelukast sodium (MKT) in their co‐formulated tablets. The method is based on measurement of the synchronous fluorescence intensities of the two drugs in McIlvaine's buffer, pH 2.3, in the presence of carboxy methyl cellulose sodium (CMC) as a fluorescence enhancer at a constant wavelength difference (Δλ) of 160 nm. The presence of CMC enhanced the synchronous fluorescence intensity of DSL by 216% and that of MKT by 28%. A linear dependence of the concentration on the amplitude of the second derivative synchronous fluorescence spectra was achieved over the ranges of 0.10–2.00 and 0.20–2.00 µg/mL with limits of detection of 0.02 and 0.03, and limits of quantification of 0.05 and 0.10 µg/mL for DSL and MKT, respectively. The proposed method was successfully applied for the determination of the studied compounds in laboratory‐prepared mixtures and tablets. The results were in good agreement with those obtained with the comparison method. The high sensitivity attained by the proposed method allowed the determination of MKT in spiked human plasma with average % recovery of 100.11 ± 2.44 (n = 3). Copyright © 2014 John Wiley & Sons, Ltd.
Bioproduction of highly charged designer peptide surfactants via a chemically cleavable coiled‐coil heteroconcatemer
Authors: Fletcher Nicholas L. , Paquet Nicolas , Dickinson Ellyce L. , Dexter Annette F. 
Published: September 2014

ABSTRACTDesigner peptides have recently attracted attention as self‐assembling fibrils, hydrogelators and green surfactants with the potential for sustainable bioproduction. Carboxylate‐rich peptides in particular have shown potential as salt‐resistant emulsifiers; however the expression of highly charged peptides of this kind remains a challenge. To achieve expression of a strongly anionic helical surfactant peptide, we paired the peptide with a cationic helical partner in a coiled‐coil miniprotein and optimized the polypeptide sequence for net charge, hydropathy and predicted protease resistance (via the Guruprasad instability index). Our design permitted expression of a soluble concatemer that accumulates to high levels (22% of total protein) in E. coli. The concatemer showed high stability to heat and proteases, allowing isolation by simple heat and pH precipitation steps that yield concatemer at 133 mg per gram of dry cell weight and >99% purity. Aspartate‐proline sites were included in the concatemer to allow cleavage with heat and acid to give monomeric peptides. We characterized the acid cleavage pathway of the concatemer by coupled liquid chromatography‐mass spectrometry and modeled the kinetic pathways involved. The outcome represents the first detailed kinetic characterization of protein cleavage at aspartate‐proline sites, and reveals unexpected cleavage preferences, such as favored cleavage at the C‐termini of peptide helices. Chemical denaturation of the concatemer showed an extremely high thermodynamic stability of 38.9 kcal mol−1, with cleavage decreasing the stability of the coiled coil to 32.8 kcal mol−1. We determined an interfacial pressure of 29 mN m−1 for both intact and cleaved concatemer at the air‐water interface, although adsorption was slightly more rapid for the cleaved peptides. The cleaved peptides could be used to prepare heat‐stable emulsions with droplet sizes in the nanometer range. Biotechnol. Bioeng. 2015;112: 242–251. © 2014 Wiley Periodicals, Inc.
Development and validation of an LC‐APCI‐MS/MS method for the determination of phenethyl isothiocyanate in human plasma
Authors: Zheng Li. , Zheng Feng 
Published: September 2014

AbstractPhenethyl isothiocyanate (PEITC) is a promising chemopreventive agent present in cruciferous vegetables. This paper describes the development of a method for the determination of PEITC in human plasma by liquid chromatography/tandem mass spectrometry (LC‐MS/MS). Atmospheric‐pressure chemical ionization was found more suitable for ionization of PEITC than electrospray ionization. Because of the lability of PEITC, a combination of low temperature and acidification was applied to minimize the degradation during the sample collection and preparation procedure. A simple protein precipitation with acetonitrile was used for the preparation of plasma samples. The analyte and 1‐phenylpropyl isothiocyanate as internal standard (IS) were subjected to chromatographic analysis on a C18 column (50 × 2.1 mm, 5 µm) using 85% methanol as mobile phase at a flow rate of 0.3 mL/min. The total analysis time for each chromatograph was 3 min and the results were linear over the studied range (5.00–250 ng/mL). The intra‐ and inter‐day precision values were acceptable as per US Food and Drug Administration guidelines. This method was successfully applied in the determination of PEITC concentrations in plasma samples from healthy chinese Volunteers. Copyright © 2014 John Wiley & Sons, Ltd.
Comparative efficacy of different acute reperfusion therapies for acute ischemic stroke: a comprehensive benefit–risk analysis of clinical trials
Authors: Tsivgoulis Georgios , Alleman John , Katsanos Aristeidis H. , Barreto Andrew D. , Kohrmann Martin , Schellinger Peter D. , Molina Carlos A , Alexandrov Andrei V. 
Published: September 2014

AbstractBackgroundNumerous acute reperfusion therapies (RPT) are currently investigated as potential new therapeutic targets in acute ischemic stroke (AIS). We conducted a comprehensive benefit–risk analysis of available clinical studies assessing different acute RPT, and investigated the utility of each intervention in comparison to standard intravenous thrombolysis (IVT) and in relation to the onset‐to‐treatment time (OTT).MethodsA comprehensive literature search was conducted to identify all available published, peer‐reviewed clinical studies that evaluated the efficacy of different RPT in AIS. Benefit‐to‐risk ratio (BRR), adjusted for baseline stroke severity, was estimated as the percentage of patients achieving favorable functional outcome (BRR1, mRS score: 0–1) or functional independence (BRR2, mRS score: 0–2) at 3 months divided by the percentage of patients who died during the same period.ResultsA total of 18 randomized (n = 13) and nonrandomized (n = 5) clinical studies fulfilled our inclusion criteria. IV therapy with tenecteplase (TNK) was found to have the highest BRRs (BRR1 = 5.76 and BRR2 = 6.82 for low‐dose TNK; BRR1 = 5.80 and BRR2 = 6.87 for high‐dose TNK), followed by sonothrombolysis (BRR1 = 2.75 and BRR2 = 3.38), while endovascular thrombectomy with MERCI retriever was found to have the lowest BRRs (BRR1 range, 0.31–0.65; BRR2 range, 0.52–1.18). A second degree negative polynomial correlation was detected between favorable functional outcome and OTT (R2 value: 0.6419; P < 0.00001) indicating the time dependency of clinical efficacy of all reperfusion therapies.ConclusionIntravenous thrombolysis (IVT) with TNK and sonothrombolysis have the higher BRR among investigational reperfusion therapies. The combination of sonothrombolysis with IV administration of TNK appears a potentially promising therapeutic option deserving further investigation.