bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case of Acquired Lymphangioma of the Vulva
Authors: Akimoto Kayoko , Nogita Toshitatsu , Kawashima Makoto 
Published: April 2015

AbstractA 46‐year‐old woman developed multiple papules on the right labium majus. A histological examination revealed acanthotic epidermis and dilated vessels in the papillary dermis. The clinical and histological features were compatible with those of lymphangiectasis or acquired lymphangioma of the vulva, which occurs after surgery or irradiation for cervical cancer. This patient, however, had no such past history. Acquired lymphangioma of the vulva arising without obvious causes seems to be unusual.
Cutaneous Eruptions Induced by Granulocyte Colony‐stimulating Factor in Two Cases of Acute Myelogenous Leukemia
Authors: Yamashita Noriko , Natsuaki Masaru , Morita Hideki , Kitano Yukio , Sagami Seiichiro 
Published: April 2015

AbstractRecombinant human granulocyte colony‐stimulating factor (rhG‐CSF) induced cutaneous eruptions in two cases of acute myelogenous leukemia. In both cases, the eruptions appeared during rhG‐CSF therapy for neutropenia induced by the remission‐induction chemotherapy and disappeared rapidly after the discontinuance of rhG‐CSF therapy. Histopathology of those eruptions revealed dermal cell infiltrations consisting of some neutrophils and atypical cells. It was interesting that, although there were no leukemic cells in the peripheral blood or bone marrow, eruptions containing many leukemic cells appeared. The mechanism of the appearance of these eruptions was unclear, but it was considered that a few leukemic cells might have responded to rhG‐CSF and proliferated in the skin.
A Case of Herpes‐associated Erythema Multiforme in a Japanese Child
Authors: Uemura Tomoko , Nagayama Michiyo , Kawashima Makoto 
Published: April 2015

AbstractA case of a five‐year‐old Japanese boy with herpes‐associated erythema multiforme (HAEM) was reported. The patient had eleven recurrences of herpes labiaris within one year; four of these recurrences were accompanied by erythema multiforme. A study of the human leukocyte antigens revealed the presence of HLA‐DQW3, which has been reported to be significantly frequent in Caucasian patients with HAEM. Oral administration of acyclovir at the onset of herpes labialis was effective in preventing HAEM. Early administration of oral steroids at the onset of HAEM also prevented its exacerbation.
Current Topics of Immunohistochemistry as Applied to Skin Tumors
Authors: Hashimoto Ken , Fujiwara Keiko , Mehregan Amir 
Published: April 2015

AbstractSuppressor oncogene p53 is expressed more frequently in the nodular portion than the superficial spreading portion of the same melanoma. PCNA expression follows the same pattern but it is strongly expressed already in the superficial spreading portion. CD34 is found to label dermatofibrosarcoma protuberans. There is the mixture of negative strands but the immunostains are predominantly positive in the three cases studied. Neurogenic tumors are reactive but the intensity of staining is only moderate. Fibrous histiocytoma, dermatofibroma and other fibrohistiocytic tumors are non‐reactive. CD34 is expressed in the outer root sheath of hair follicle below the sebaceous gland level. A case of piloepidermal cyst is CD34 positive. CD34 is expressed in vascular endothelial cells; not only hemangiomas but also lymphangiosarcoma is CD34 positive. A case of indeterminate cell histiocytosis following scabies and superficial variant of clear cell sarcoma are discussed as examples of new entities.
A Case of Lupus Meningitis Treated Successfully with Methylprednisolone Pulse Therapy
Authors: Kanekura Takuro , Mizumoto Junichi , Setoyama Mitsuru 
Published: April 2015

AbstractA 46‐year‐old female had suffered from systemic lupus erythematosus (SLE) for 8 years. Headache, vomiting and stiff neck appeared in the active phase of SLE. Findings in the cerebrospinal fluid were consistent with those of lupus meningitis. No pathogenic microbes were detected by microbiological or immunological examinations. She was diagnosed as having lupus meningitis.The method discussed herein which elucidates the cause of fever in SLE using white blood cell count (WBC) and alpha‐2 globulin appeared to be useful for examining this case of meningitis.Lupus meningitis seems to preferentially occur in SLE patients with positive anti‐ribonucleoprotein (RNP) antibody.Pulse therapy with methylprednisolone appeared to work well in this lupus meningitis patient who had had a long course of corticosteroid therapy.
Metastatic Behavior and Tumorigenicity of a Human Melanoma Cell Line (MM‐RU) after Injection into Nude Mice
Authors: Stender Ida‐Marie , Etoh Takafumi , Tsuchida Tetsuya , Nakagawa Hidemi , Byers H. Randolph , Imokawa Genji , Ishibashi Yasumasa 
Published: April 2015

AbstractThe ability of the human amelanotic melanoma cell line MM‐RU to produce experimental metastases and to grow tumors at subcutaneous inoculation sites in 4‐week‐old nude mice was examined.After i.v. inoculation of 106 cells, all injected mice (n=21) developed consistent numbers of metastatic pulmonary colonies within 32 days. The coefficients of variation for the number of colonies were between 17%–23% in three independent experiments. Survival time after i.v. inoculation was 63 ± 7 days (mean ± SD) (n=20). Within 20 days, subcutaneous inoculation of 5 × 106 cells resulted in tumor growths of 13 ± 3 mm (mean ± SD) at the inoculation sites in all nude mice (n=12).The MM‐RU cell line seems to be a simple, fast vehicle for testing the effect of melanoma growth modulators on experimental pulmonary metastases as well as on subcutaneously growing melanoma.
Macrophage Markers 25F9 and 27E10 on Human Keratinocytes in Normal and Diseased Skin
Authors: Simon Miklós , Hunyadi János , Gómez Renate Schlegel 
Published: April 2015

AbstractIn the present study, an immunohistochemical analysis was made of the expression pattern of different macrophage markers such as 25F9, 27E10 and RM 3/1 on the surface of human keratinocytes (HK) in biopsies obtained from healthy volunteers and from patients with lichen planus, chronic cutaneous graft‐versus‐host reaction, mycosis fungoides, and purpura pigmentosa chronica. In biopsies from the healthy volunteers and from both clinically involved and uninvolved skin of the patients, the HK of the basal cell layer exhibited a specific peroxidase‐positive reaction when the monoclonal antibody against 25F9 was used. Lesional HK from all patients studied displayed 25F9 and 27E10 in nearly the entire epidermis. The present findings provide further evidence that HK and macrophages share a number of common cell surface moieties.
Pilary Complex Carcinoma: An Adnexal Carcinoma of the Skin with Differentiation towards the Components of the Pilary Complex
Authors: Rahbari Homayoon , Mehregan Amir H. 
Published: April 2015

AbstractWe report twelve cases with a distinctive form of adnexal carcinoma occurring most commonly over the head and neck of relatively old individuals. The growth was single in all cases, deep dermal in location, and consisted of massive proliferation of small basaloid cells with only occasional connection with the surface epidermis. The basaloid tumor masses showed only a few areas of palisading of their outer cell nuclei and occasional retraction space formation. The neoplasm revealed scattered areas of trichilemmal and epidermoid keratinization, foci of sebaceous, and areas of sweat ductal differentiation indicating participation of various components of the pilary complex.
Multiple Agminated Juvenile Melanoma Arising on a Hyperpigmented Macule
Authors: Akasaka Toshihide , Imamura Yuko , Kon Saiichi 
Published: April 2015

AbstractA case of multiple agminated juvenile melanoma (MAJM) arising on a hyperpigmented macule is reported. The patient, a 1‐year‐old boy, had 9 small nodules on an irregular, hyperpigmented macule on the right shoulder. Histologically, the nodules contained nests of spindle‐shaped cells in their epidermis and at the dermoepidermal junction, and the hyperpigmented macule exhibited increased melanin granules in the basal layer and small nests of epithelioid cells. We concluded that MAJM on a hyperpigmented macule is the subtype of the nevus spilus.
An Evaluation, Using Computerized Image Analysis, of Antimicrobial Efficacy of an Automatic Hand Washing Machine with Ultrasonic Wave Spraying
Authors: Nishijima Setsuko , Namura Shoko , Mitsuya Kaoru , Asada Yasuo 
Published: April 2015

AbstractWe studied the antimicrobial efficacy of hand washing with a quick‐dry hand washing machine [TE‐KIREIKI]® employing ultrasonic wave spraying and its incidental alcohol‐based product [AROKULIN‐E]®. The subjects of this study were 10 males who had been instructed not to use any antimicrobial agent for the previous 2 weeks or any hand soap for the previous 5 hours.They pressed their palms on agar before and after washing their hands. After 48‐hour incubation at 37°C, the bacterial colonies grown on the agar were counted using the [ASPECT]® image processing system. The colony count was expressed as the post‐stamp versus pre‐stamp percent (%) reduction.Two patterns of hand washing were examined in this study: [A] a 3‐second hand wash using an alcohol‐based product [AROKULIN‐E]® and [B] a 30‐second hand wash using a non‐medicated detergent soap with running water in addition to hand washing by [A].The percent (%) reduction after hand washing patterns [A] and [B] were 49.1% and 51.3%. These reduction rates indicated that these patterns did not eradicate bacteria from the hand surface. Therefore, we concluded that this quick‐dry hand washing machine employing an ultrasonic wave spraying method combined with an alcohol‐based product needs improvement.
Lipid Modification during Epidermal Cell Differentiation
Authors: Thompson Guy A. 
Published: April 2015

AbstractAs keratinocytes differentiate into corneocytes of the stratum corneum or epidermal permeability barrier, their lipids are modified so as to fulfill totally different functions. Recent experimentation has clarified the molecular mechanisms by which lipids of membrane origin are targeted to specialized lamellar bodies, where metabolic retailoring makes them suitable for use in the water‐impermeable intercellular lamellae. In this latter structure the modified lipids are bound covalently to specialized proteins in a way that encourages the formation of lipid bilayers alternating with lipid monolayers. Only now are potential clues to the molecular regulation of this dramatic lipid transformation becoming apparent.
Historical Aspects of the Oral Use of Retinoids in Acne
Authors: Hartmann Diethelm , Bollag Werner 
Published: April 2015

AbstractA number of investigations of the effects of vitamin A deficiency in animals and man and its treatment with natural products containing vitamin A were carried out in the twenties and thirties. In 1942, a clinical study in patients with acne treated with vitamin A yielded encouraging results. Further trials in the forties and fifties, trying to confirm the beneficial effect of oral vitamin A in acne, met with equivocal success. In the sixties, all‐trans retinoic acid (tretinoin) became clinically available, and its topical efficacy in acne could be demonstrated. In 1971, oral tretinoin also was shown to be active in patients with acne. Coincidentally, the efficacy of oral 13‐cis retinoic acid (isotretinoin) became evident in a series of unpublished studies in Europe. Then, in 1978, a trial carried out at the NIH, Bethesda, Maryland, yielded convincing evidence that isotretinoin is a potent new drug for the treatment of severe cystic acne.In 1982, isotretinoin was registered in the United States and one year later in Europe for the treatment of severe, recalcitrant, cystic acne. Since then, many thousands of patients suffering psychologically and physically from the severity of their disease have been treated successfully with this drug. However, the main concern of physicians prescribing isotretinoin has to focus on its potentially severe side effects, particularly its teratogenicity.
Dispersed Cell Culture of Human Sweat Duct Cells under Serum‐free Conditions
Authors: Uchida Naoyuki , Oura Hajimu , Nakanishi Hideki , Urano Yoshio , Arase Seiji 
Published: April 2015

AbstractHuman eccrine gland duct cells were successfully cultured using a serum‐free medium, K‐GM medium. Eccrine sweat ducts were isolated from dispase treated skin specimens from palms or soles. After treatment of the isolated ducts with trypsin and EDTA, dispersed cells were cultured in K‐GM medium. In primary cultures, small colonies were seen 3 to 4 days after inoculation. Then the cells rapidly proliferated and formed large colonies with a paving stone‐like cell arrangement. During the culture, small dome shaped areas were sometimes formed in the centers of colonies. Cultures multiplied for a maximum of 7 passages. The plating efficiencies of the 1st to 6th passage cells were about 20% to 30%. Immunocytochemically, cultured cells were positively stained with anti‐carcinoembryonic antigens, K8.37 and K8.13, but not with anti‐S100 protein, anti‐HLA‐DR, 34βB4, or PKK3. An electron micrograph of the cultured cells showed a multilayer of flattened cells linked by desmosomes. These results indicate that the cultured cells possessed the staining properties compatible with those of the ductal portion of eccrine sweat glands. No contamination by other mesenchymal cells, such as fibroblasts, was seen during the culture.
Ultrastructural Observation of Epithelioid Sarcoma
Authors: Kanai Takako , Irisawa Ryokichi , Kusama Hiroshi , Oh‐i Tsunao 
Published: April 2015

AbstractEpithelioid sarcoma located in the right hip‐to‐thigh region of a 58‐year‐old female was studied by light microscopy, immunohistochemistry, and electron microscopy. Light microscopy revealed both light and dark cells among the tumor cells. Round, homogeneous, eosinophilic bodies were noted extracellularly and intracellularly. Extracellular bodies were similar in size to tumor cells and were not stained by PAS or Congo red. In contrast, intercellular bodies were PAS positive and diastase resistant. Tumor cells were positive for vimentin, keratin, and, partially, for epithelial membrane antigen. Ultrastructurally, tumor cells had numerous microfilaments with tonofibril‐like arrangement, lipid droplets, and reticulogranular structure in their cytoplasm. The reticulogranular structure was similar to the tonofilaments recognized in degenerative tumor cells. Oval filamentous bodies were recognized in the stroma. They appeared to be very similar to colloid bodies derived from tonofilaments of keratinocytes. It was considered that the filamentous bodies corresponded to extracellular eosinophilic bodies and showed signs of fibrous necrosis of tumor cells. Intracellular eosinophilic bodies were considered to be lipid droplets.
Spontaneous Contraction of Leukodermic Patches in Waardenburg Syndrome
Authors: Chang Tsuling , Hashimoto Ken , Bawle Erawati V. 
Published: April 2015

AbstractWaardenburg Syndrome is an autosomal dominantly inherited disorder with variable penetrance (1–5). It is a rare disorder with an estimated frequency of 1:20,000 in Kenya (East Africa) and 1:40,000, in the Netherlands presenting with or without deafness. The frequency with deafness is lower, estimated at 1:50,000 to 1:212,000 (1, 2). The major characteristic features are as follows, with reported incidences in parenthesis: 1) Dystopia canthorum (99%); 2) synophrys (17%–69%); 3) broad nasal root (78%); 4) depigmentation of hair, skin, or both (17%–58% with white forelock); 5) heterochromic or hypochromic irides (greater than 20%); 6) congenital deafness (9%–38%) (1–7). Genetic heterogeneity has led to classification of affected families as type I, with dystopia canthorum, or type II, without dystopia canthorum (2, 6). Piebaldism and Woolf's Syndrome can present with pigmentary changes which are similar to Waardenburg Syndrome (2, 3, 8). Woolf's Syndrome also includes deafness (9). However, the distinguishing structural ophthomologic abnormalities of dystopia canthorum, broad nasal root, and synophrys are not found in either piebaldism or Woolf's Syndrome (2, 3, 8, 9). The congenital patterns of leukoderma in both piebaldism and Waardenburg Syndrome has been believed to be stable throughout the lifetime of the affected individuals (2, 10). We report an otherwise typical family with Waardenburg Syndrome, type I, in which 2 members atypically demonstrate spontaneous pigmentation and contraction of congenital leukodermic patches. To our knowledge, this has not been previously reported in Waardenburg Syndrome (1, 2, 6, 10).
Folliculosebaceous Cystic Hamartoma Associated with Rosacea
Authors: Yamamoto Toshiyuki , Ohkubo Hiroko , Nishioka Kiyoshi 
Published: April 2015

AbstractA case of folliculosebaceous cystic hamartoma associated with rosacea is presented. Clinically, a solid, 5‐mm‐sized nodule was observed on the nose which showed telangiectasia and a waxy appearance. Histologically, a large horn cyst was located in the dermis, and was continuous with the surface epidermis. It was associated with mature sebaceous glands and immature hair follicles. Folliculosebaceous cystic hamartoma and similar cases have recently been reported, but this is the first report accompanied by rosacea.
High‐dose Tocopherol Acetate Therapy in Epidermolysis Bullosa Siblings of the Cockayne‐Touraine Type
Authors: Shirakata Yuji , Shiraishi Satoshi , Sayama Koji , Shinmori Hideyo , Miki Yoshiharu 
Published: April 2015

AbstractWe have employed a successful therapy for epidermolysis bullosa with high‐dose oral tocopherol acetate. Two siblings with dominant dystrophic epidermolysis bullosa of the Cockayne‐Touraine type were reported. Both siblings suffered from tense blisters and erosions healing with scars and transient milia on the extremities. Electron microscopic study of the blisters revealed a cleavage beneath the lamina densa in both siblings. High‐dose oral tocopherol acetate therapy was administered to them with satisfactory clinical reduction of the blister formation.
Serum Levels of Interleukin 6 in Patients with Pustulosis Palmaris et Plantaris
Authors: Nakamura Takehiko , Oishi Munashi , Johno Masayoshi , Ono Tomomichi , Honda Mitsuo 
Published: April 2015

AbstractSerum interleukin 6 (IL‐6) was measured in patients with pustulosis palmaris et plantaris by a fluorescent sandwich enzyme‐linked immunosorbent assay (ELISA) method. Increased production of IL‐6 was observed in 8 cases out of 10 showing numerous pustules in their active stage. Six cases were measured before and after tonsillectomy, and statistically significant reduction in the levels of IL‐6 paralleled an improvement in the disease activity. It is suggested that IL‐6 may be one of the mediators which link tonsillectomy to the activity of pustulosis palmaris et plantaris.
Clinical Criteria for Differentiating between Recessive and Dominant Forms of Dystrophic Epidermolysis Bullosa, Elaborated from an Analysis of 119 Cases
Authors: Nomura Kazuo , Imaizumi Takashi , Sato Takashi , Hashimoto Isao 
Published: April 2015

AbstractWe attempted to establish clinical criteria which differentiated between recessive dystrophic epidermolysis bullosa (RDEB) and dominant DEB (DDEB), since these two groups show prominent differences in prognosis, genetic recurrence risk and response to some types of treatment. The total of 119 cases examined consisted of our own 9 cases (2 of RDEB and 7 of DDEB) and 110 cases (26 of RDEB and 84 of DDEB) collected from the medical literature. They were analyzed by calculating the sensitivity and specificity of ten clinical features. We concluded that, syndactyly, presence of complications, dental lesions, remission‐less course, and oral lesions are strongly indicative of RDEB (more than 70% specific). Intractable skin ulcer is suggestive of RDEB (more than 55% specific). Nail lesions, scar and atrophy, milia and pigmentation are not helpful in the differentiation because of their commonality. The proposed criteria are simple, reliable, and practical, providing us with a useful tool for differentiation of RDEB and DDEB in daily practice.
An Analysis of 24 Patients with IgA Deposition at the BMZ
Authors: Meng‐Wu Weng , Bing‐Sen Qiu , Ke‐Fei Kang 
Published: April 2015

AbstractA study of 24 patients with IgA deposition at the BMZ of the skin showed that five conditions could be recognized: 1) linear IgA bullous dermatosis in adults (LAD, 7 cases); 2) linear IgA and IgG bullous dermatosis in adults (LAGD, 10 cases); 3) chronic bullous disease of childhood (CBDC, 3 cases); 4) dermatitis herpetiformis (DH, 1 case), and 5) systemic lupus erythematosus (SLE, 3 cases). Histopathologically, 5 of 7 patients with LAD were similar to the DH group, but 7 of 10 patients with LAGD were similar to the BP group. Half the patients with LAD and LAGD had oral lesions, and most of them had excellent responses to dapsone and Tripterygium Wilfordii, but the patients with CBDC did not respond to these treatments. In the patients with LAD and LAGD, the positivity rates of IgA anti‐BMZ antibodies examined by indirect immunofluorescence (IIF) on intact skin and NaCl split skin were 41% and 64%, respectively. The heterogeneity of the histopathologic pictures of LAD and LAGD, the incidence of DH, and the value of using NaCl split skin for IIF are discussed.
A Correlation between Carotenemia and Biliary Dyskinesia
Authors: Nishimura Toshio 
Published: April 2015

AbstractThe interrelationship between carotenemia and biliary dyskinesia was studied in eighty‐two biliary dyskinesia patients and twenty‐seven normal subjects. Incidence of serum carotene levels of more than 300 µg/dl was more frequent in biliary dyskinesia patients without liver damage or hyperlipemia. Among the carotenemia patients, no dietary cases were found, but metabolic and hyperlipemic ones were observed. Significant relationships were found between gall bladder contraction rate and the levels of serum carotene, vitamin A, and lipids in metabolic carotenemia. A close relationship between metabolic carotenemia and biliary dyskinesia was strongly suggested.
Association of Generalized Granuloma Annulare with Autoantibodies
Authors: Tada Joji , Seno Akemi , Ueda Masumi , Arata Jirô , Nagao Yo 
Published: April 2015

AbstractGranuloma annulare is a degenerative disease of the skin histopathologically characterized by focal degeneration of collagen with a surrounding infiltrate of lymphoid cells, histiocytic cells, and multinucleated giant cells. Immunological abnormalities such as delayed‐type hypersensitivity and vasculitic origin are suspected in the pathogenesis. We describe three patients with generalized granuloma annulare, in whom autoantibodies, including antinuclear antibody, antithyroid stimulating hormone receptor antibody, and immune complex, were detected.
A Case of Cutaneous B‐cell Lymphoma with a Storiform Stromal Reaction
Authors: Nagatani Tetsuo , Miyazawa Megumi , Matsuzaki Toshiko , Iemoto Gaijiro , Kim Shu‐Taku , Baba Naoko , Miyamoto Hideaki , Nakajima Hiroshi 
Published: April 2015

AbstractA case of cutaneous B‐cell lymphoma is described. The patient was treated only by surgical excision of the skin tumors five times during a period of about two years from February of 1984 to October of 1986. After the last surgical excision, a continuous disease‐free period was achieved. Biopsy samples showed dense lymphocytic infiltrations with discrete masses in the dermis and subcutis; one of them showed a storiform pattern. At the time, the infiltrating cells were composed of medium and large lymphoid cells and spindle‐shaped cells. The medium and large lymphoid cells were positive for CD20, CD22 and HLA‐DR and negative for CD3, CD4, CD5, CD8, CD43, and kappa and lambda light chain. The spindle‐shaped cells were negative for CD20, CD43, kappa and lambda light chain, lysozyme, and S‐100 protein.