bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification and analysis of oxidative stress‐related genes in hypoxic‐ischemic brain damage using bioinformatics and experimental verification
Authors: Jin Ni , Sha Sha , Ruan Yanghao , Ouyang Ying 
Published: August 2024

AbstractBackgroundOxidative stress (OS) plays a major role in the progress of hypoxic‐ischemic brain damage (HIBD). This study aimed to investigate OS‐related genes and their underlying molecular mechanisms in neonatal HIBD.MethodsMicroarray data sets were acquired from the Gene Expression Omnibus (GEO) database to screen the differentially expressed genes (DEGs) between control samples and HIBD samples. OS‐related genes were drawn from GeneCards and OS‐DEGs in HIBD were obtained by intersecting with the DEGs. Subsequently, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Enrichment Analysis (GSEA) were conducted to determine the underlying mechanisms and functions of OS‐DEGs in HIBD. Moreover, the hub genes were screened using the protein−protein interaction network and identified in the GSE144456 data set. CIBERSORT was then performed to evaluate the expression of immunocytes in each sample and perform a correlation analysis of the optimal OS‐DEGs and immunocytes. Finally, quantitative reverse transcription polymerase chain reaction (RT‐qPCR) and immunohistochemistry were performed to validate the expression levels of the optimal OS‐DEGs.ResultsIn total, 93 OS‐DEGs were identified. GO, KEGG, and GSEA enrichment analyses indicated that these genes were predominantly enriched in OS and inflammation. Four OS‐related biomarker genes (Jun, Fos, Tlr2, and Atf3) were identified and verified. CIBERSORT analysis revealed the dysregulation of six types of immune cells in the HIBD group. Moreover, 47 drugs that might target four OS‐related biomarker genes were screened. Eventually, RT‐qPCR and immunohistochemistry results for rat samples further validated the expression levels of Fos, Tlr2, and Atf3.ConclusionsFos, Tlr2 and Atf3 are potential OS‐related biomarkers of HIBD progression. The mechanisms of OS are associated with those of neonatal HIBD.
Forsythiaside A ameliorates bleomycin‐induced pulmonary fibrosis by inhibiting oxidative stress and apoptosis
Authors: Yang Fan , Zhang Qinqin , Wang Xi , Hu Yingbo , Chen Suiqing 
Published: August 2024

AbstractBackgroundPulmonary fibrosis (PF) is a common clinically critical disease characterized by high morbidity and high mortality. Forsythiaside A (FA) is a phenylethanol glycoside component in Forsythia suspensa, which has anti‐inflammatory, antioxidant, and antiviral activities. However, the effects of FA on bleomycin (BLM)‐induced PF are unclear.PurposeThe present study explored the role of FA in the amelioration of oxidative stress and apoptosis in BLM‐induced PF as well as the possible underlying mechanisms, in vivo and in vitro.MethodsNetwork pharmacology was used to collect the effects of FA on BLM‐induced PF. Subsequently, further observation of the effects of FA on mice with PF by pulmonary pathological changes, transmission electron microscopy, real‐time polymerase chain reaction, Western blot analysis, immunofluorescence, and immunohistochemistry. An in vitro model was constructed by inducing A549 with transforming growth factor beta‐1 (TGF‐β1) to observe the effect of FA on epithelial cell apoptosis.ResultsNetwork pharmacology predicted signaling pathways such as IL‐17 signaling pathway and Relaxin signaling pathway. The results of in vivo studies showed that FA ameliorated BLM‐induced PF through inhibition of fibrosis, modulation of apoptosis, and oxidative stress. In addition, FA promoted TGF‐β1‐induced apoptosis in A549 cells.ConclusionsThe results of our study suggested that FA could protect mice against BLM‐induced PF by regulating oxidative stress and apoptosis as well as the Epithelial mesenchymal transition pathway.
Switching cisplatin to carboplatin in chemotherapy for metastatic penile cancer in a patient intolerant to cisplatin
Authors: Sekine Keita , Suyama Takahito , Takei Kazuki , Kato Hiroto , Wakai Ken , Okato Atsushi , Hou Kyokushin , Araki Kazuhiro , Yamazaki Kazuto , Naya Yukio 
Published: August 2024

IntroductionCisplatin is currently the key drug in the chemotherapy regimen for metastatic penile cancer. There are few reports of alternative medicines for patients who cannot tolerate cisplatin. This report describes a case in which carboplatin was used instead.Case presentationThe patient presented with a chief complaint of edema in the groin area. On close examination, penile cancer (cT2‐3N3M0 stage IV) with pelvic lymph node metastasis was diagnosed. He was started on chemotherapy with cisplatin (50 mg/m2 on days 1 and 2), paclitaxel (120 mg/m2 on day 1), and 5‐fluorouracil (1000 mg/m2 on days 2–5), but he developed acute kidney failure on the 12th day, thought to be caused by cisplatin. Cisplatin was changed to carboplatin, and chemotherapy was continued. He has received nine courses of chemotherapy and is doing well.ConclusionA case of penile cancer safely and effectively treated with chemotherapy using carboplatin was reported.
First‐class universities, economic development, and the middle‐income trap
Authors: Chang Jinxiong , Sun Yan , Zhang Liuchen 
Published: August 2024

AbstractFirst‐class universities play an extremely important role in cultivating high‐quality talents and technological innovation, serving as a significant indicator of a country's level of higher education development, developmental strength, and potential. However, there is little literature studying the long‐term impact of first‐class universities on a country's economic development. To better understand this long‐term influence, our study examines the effect of first‐class universities on per capita income based on cross‐national samples, particularly their role in overcoming the “middle‐income trap,” and analyzes whether general higher education can bring about equivalent effects. The main research conclusions are: First, both general higher education and first‐class universities can significantly improve a country's per capita income, but compared to general higher education, first‐class universities have greater marginal effects on national per capita income, and can more effectively enhance domestic average income levels and promote sustainable economic growth over time; Second, first‐class universities have the greatest marginal effect on improving per capita income in middle‐income countries, and compared to general higher education, first‐class universities play a larger role in helping developing countries break through the “middle‐income trap”; Third, both general higher education and first‐class universities positively affect innovative activities, but first‐class universities play a more significant role in promoting technological innovation, which can better facilitate high‐quality economic development. Our study not only enhances the understanding of the effects and differences between general higher education and first‐class universities on long‐term economic development, but also contributes to the understanding of the economic miracle that China has created since its reform and opening up. It also provides clear policy implications.
Beitrag zur Kenntnis zentralperuanischer Pflanzengesellschaften III Pflanzengesellsehaften der subalpinen Stufe
Authors: Gutte Peter 
Published: August 2024

ZusammenfassungIn Fortsetzung der Arbeiten über zentralperuanische Pflanzengesellschaften (Gutte 1978, 1980) werden die Vegetationseinheiten der subulpinen Stufe von 3600–3900 m Höhe aus der Umgebung von Canchayllo (Dpt. Junin, Prov. Yauli, 30 km SSO La Oroya) beschrieben. Basierend auf 216 pflanzensoziologischen Aufnahmen werden 11 Assoziationen und 19 weitere Pflanzengesellschaften unterschieden. Sie umfassen das trockene Weideland der Klasse Stipetea mucronatae, das feuchte bis nasse Grasland der Klasse Plantaginetea australis, die Felsvegetation und die rein anthropogen bedingten Pflanzengesellschaften. Von jeder Gesellschaft werden die Standortsverhältnisse, der Gesellschaftsaufbau, die Lebensformen, z. T. auch Untergesçllschaften und landwirtschaftlicher Wert angegeben.
Comparative transcriptomics suggests a potential realizator gene for carapace expansion in longtail tadpole shrimp, Triops longicaudatus (Branchiopoda: Notostraca)
Authors: Jung Seunghun , Kim Seojun , Shin Seunggwan 
Published: August 2024

ABSTRACTThe origin of morphological innovation has been extensively studied within evolutionary developmental biology (evo‐devo). Recent studies have demonstrated that the developmental module for double‐layered epithelial outgrowths is conserved between the insect wings and branchiopod crustacean carapace, thereby introducing homology among these diverse structures. However, evo‐devo studies on the branchiopod crustacean carapace have been primarily limited to a single species, the water flea Daphnia magna, leaving the gene regulatory network governing carapace development not comprehensively understood. Furthermore, realizator genes downstream of the character identity mechanism (ChIM) for bilayered epithelial development remain inadequately described. In this study, we analyzed tissue‐specific transcriptional profiles in the developing longtail tadpole shrimp, Triops longicaudatus. We observed significant upregulation of papilin in the carapace‐bearing head, along with its expression in both the carapace and the trunk limb lobes. Based on these results, we hypothesize that differential expression of papilin is involved in the disproportional growth of Triops carapace. Our findings will contribute to elucidating the diversification of double‐layered epithelial outgrowths across distant arthropod lineages.
Expanding the genetic and clinical spectrum of SLC25A42‐associated disorders and testing of pantothenic acid to improve CoA level in vitro
Authors: Heckmann Katharina , Iuso Arcangela , Reunert Janine , Grüneberg Marianne , Seelhöfer Anja , Rust Stephan , Fiermonte Giuseppe , Paradies Eleonora , Piazzolla Carmela , Mannil Manoj , Marquardt Thorsten 
Published: August 2024

AbstractSLC25A42 encodes the mitochondrial coenzyme A (CoA) transporter localized at the inner mitochondrial membrane. SLC25A42 deficiency leads to a congenital disease with a heterogeneous clinical presentation, including myopathy, developmental delay, lactic acidosis, and encephalopathy. Twenty‐one patients have been described so far. In the current study, we report on the identification of new biallelic variants in SLC25A42 in three siblings. Patients presented with symmetrical T2 hyperintensity of the putamen with minor volume depression at the brain MRI, elevated lactate, reduced oxygen consumption rates in muscle and fibroblasts, and reduced CoA levels in fibroblasts. Administration of pantothenic acid led to clinical stabilization and increased CoA levels in fibroblasts, thus confirming a role for SLC25A42 in energy metabolism and CoA homeostasis.
Anal HPV prevalence in individuals with and without other concomitant sexually transmitted infections
Authors: Rizzo Alberto , Moschese Davide , Salari Federica , Giacomelli Andrea , Cavallo Andrea , Cossu Maria Vittoria , Morelli Loriana , Fusetti Chiara , Carrozzo Giorgia , Reato Serena , Micheli Valeria , Antinori Spinello , Lombardi Alessandra , Gori Andrea , Gismondo Maria Rita 
Published: August 2024

AbstractThe association between human papillomavirus (HPV) and other sexually transmitted infections (STIs) in anal lesions still remains unclear. Aim of the study was to evaluate the prevalence of simultaneous infection of HPV and Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis in individuals screened for HPV anal infection. A total of 507 anal samples were tested for both anal HPV and STIs: 16% resulted positive for one or more non‐HPV STIs. Specifically, C. trachomatis, M. genitalium, and N. gonorrhoeae were detected in 8%, 5%, and 4% of cases, respectively. Two groups were considered, including a positive STI group and a negative STI group. The prevalence of HPV was similar in patients in both groups: high risk (HR)‐HPV and low risk (LR)‐HPV were 67% and 53% versus 62% (p = 0.361) and 54% (p = 0.864) of patients, respectively. However, HPV 16, 18, 35, 51, 59, and 69 were significantly more frequent in patients tested positive for other STIs versus HPV infection alone (p < 0.05). No significant differences between the two groups were observed in vaccination coverage, 28% versus 32% (p = 0.463), and HIV status, 86% versus 84% (p = 0.658). The study shows that the overall HPV status is not directly correlated to other STIs in the investigated population, except for certain HPV types, including HR‐HPV 16, reinforcing the urge for a greater vaccination coverage.
Synthesis and properties of sodium stearyl polyoxypropylene acetates
Authors: Hu Xueyi , Zhu Ying , Shi Liwen , Zhou Kaiqiang , Fang Yun , Li Fuyi 
Published: August 2024

AbstractSodium stearyl polyoxypropylene acetates (SC18PpC, p = 3, 6, 9) were synthesized through a two‐step process involving propoxylation of stearyl alcohol and subsequent carboxymethylation. Stearyl polyoxypropylene acetic acids (C18PpC, p = 3, 6, 9), the acidified products of SC18PpC, were characterized by FT‐IR, 1H‐NMR, and ESI‐MS. The properties of SC18PpC were extensively investigated and compared with sodium dodecanoate (SD) and sodium stearate (SS). The solubility of SC18PpC in cold water was enhanced. Kraft temperature (Tk) of SC18P3C is 40°C which is significantly lower than SS, and Tk of SC18PpC (p = 6, 9) are even less than 0°C. SC18PpC exhibits overall greater hard water resistance compared to SD and SS with the hardness of 0 to 450 mg/L. The hard water resistance of SC18PpC escalates proportionally with the length of the spacer, and the aqueous solution of SC18P9C retains its clarity even when exposed to the hard water of 450 mg/L, the maximum hardness level of Chinese tap water. SC18PpC demonstrates efficiency and effectiveness in reducing surface tension, as well as better foaming, emulsification and wetting power compared to SD and SS. These improved properties make SC18PpC (p = 3, 6) more effective in detergency on three typical soiled fabrics compared to that of sodium soap. Specifically, the detergency to sebum‐stained fabric of SC18P3C exhibits 1.5 times and 1.3 times improvement compared to SD and SS, respectively. Therefore, it is recommended to incorporate SC18PpC (p = 3, 6) in the detergent formulations for washing intimate apparel in tap water.
Strategies for coping with business travel stressors: Enhancing business travel satisfaction through leisure activities
Authors: Park Soona 
Published: August 2024

AbstractBusiness travel often introduces stressors due to heavy workloads and the disruption of personal routines. As a stress‐coping strategy, different types of leisure can be integrated into business travel to promote travelers' overall travel satisfaction. However, the traditional belief that more physically active leisure is always better may not hold true for business travelers; their constrained schedules and potential conflicts with employers may limit the feasibility of such activities. Using an exploratory sequential design, this study first employed semi‐structured interviews to identify four preferred leisure types among business travelers. Subsequently, an online survey showed the effectiveness of cultural/natural attractions and social leisure activities in reducing travel stress while boosting satisfaction, aligning with business travelers' primary motives. This finding supports the arousal theory, emphasizing varying desires for arousal based on the specific circumstances of travelers. This study can also contribute to the enhancement of business travelers' travel competency.
The presence of an aberrant anterior tibial artery does not depend on the patient's morphotype
Authors: Schuster Philipp , Mayer Philipp , Cornacchini Jonathan , Schlumberger Michael , Leiprecht Janina , Richter Joerg , Micicoi Grégoire 
Published: August 2024

AbstractPurposeThe aberrant anterior tibial artery (ATA) runs along the posterior surface of the tibial cortex making it, particularly, at risk during high tibial osteotomy (HTO). This study aimed to analyze the prevalence of the ATA according to global morphotype and its anatomical features in consideration of knee surgery.MethodsThis retrospective study included 1589 knees on magnetic resonance imaging (MRI) studies with long‐leg radiographs. The anatomical characteristics of the ATA, its distance in surgical areas at risk and its lumen were defined on MRI. Its presence according to the patient's morphotype (varus, valgus or neutral) was assessed using a χ2 test.ResultsThe ATA was present in 33 of 1589 knees, resulting in a prevalence of 2.1%. The anteroposterior distance of the ATA to the tibial head was 6.6 ± 2.5 mm at the height of the tibial plateau, 2.7 ± 1.6 mm at the footprint of the posterior cruciate ligament and 1.0 ± 0.6 mm under the top of the fibula (p < 0.01). For these three levels, the ratio between the distance from the ATA to the lateral tibial border and the total tibial width decreases progressively (p < 0.001). The mean lumen area of the ATA was 4.2 ± 2.5 mm2, and the lumen of the popliteal artery was 9.2 ± 3.6 mm2, representing a ratio of 49.4 ± 27.0%. The prevalence of ATA was similar between varus and valgus patients (n.s).ConclusionThe presence of an ATA is rare, found in one out of 50 cases, but can be easily identified on MRI in patients before knee surgery, especially before HTO. The patient's morphotype did not influence its presence, the lumen diameter is highly variable and its clinical impact has yet to be determined.Study DesignCross‐sectional study.Level of EvidenceLevel 3.
Type and morphology affect the success rate of bronchoscopy for postintubation tracheal stenosis
Authors: Yang Mingyuan , Li Hong , Zhou Yunzhi , Wei Huafeng , Cheng Qinghao 
Published: August 2024

AbstractObjectiveWith advancements in respiratory interventional techniques, bronchoscopic intervention technology has emerged as a viable approach for managing postintubation tracheal stenosis (PITS). However, there was a paucity of research investigating the potential impact of stenosis characteristics and morphology (such as stenosis degree, length, type, and morphology) on bronchoscopic intervention treatment prognosis for PITS patients. This study was to assess the impact of various preoperative stenosis characteristic factors on the bronchoscopic cure rate among patients.MethodsThis is a retrospective study analyzing the medical records of patients with PITS who received bronchoscopic intervention at the tertiary interventional pulmonology center.ResultsAmong the cases, 115 individuals achieved a in a success rate of 79.86% for bronchoscopic intervention therapy and were assigned to Group S. On the other hand, 29 cases required surgical intervention, accounting for a surgical treatment rate of 20.14% and were assigned to Group F. The stenosis in the Group F predominantly exhibited irregular shapes with scar granulation accompanied by tracheal chondromalacia collapse. Patients in group S experienced fewer total procedures, rigid bronchoscopy treatment, intraoperative hypoxemia, needed emergency re‐bronchoscopy in 24 h and transferred to ICU postoperatively. Patients with pure scar and granuloma, the rate of bronchoscopic success cure was higher than patients with scar granulation accompanied by tracheal chondromalacia (odds ratio: 8.208; 95% confidence interval: 2.755–24.459), and regular stenosis morphology was associated with a higher bronchoscopic success cure rate (odds ratio: 9.463; 95% confidence interval: 3.128–28.623).ConclusionIrregular airway stenosis, chondromalacia or airway collapse are key factors affecting the success rate of bronchoscopic treatment for post‐intubation tracheal stenosis.Level of evidence: 4 (historically controlled studies).
Extraction of high‐molecular‐weight DNA from Streptococcus spp. for nanopore sequencing in resource‐limited settings
Authors: Das Suvra , Delamare‐Deboutteville JérÔme , Barnes Andrew C. , Rudenko Oleksandra 
Published: August 2024

AbstractThe long‐read sequencing platform MinION, developed by Oxford Nanopore Technologies, enables the sequencing of bacterial genomes in resource‐limited settings, such as field conditions or low‐ and middle‐income countries. For this purpose, protocols for extracting high‐molecular‐weight DNA using nonhazardous, inexpensive reagents and equipment are needed, and some methods have been developed for gram‐negative bacteria. However, we found that without modification, these protocols are unsuitable for gram‐positive Streptococcus spp., a major threat to fish farming and food security in low‐ and middle‐income countries. Multiple approaches were evaluated, and the most effective was an extraction method using lysozyme, sodium dodecyl sulfate, and proteinase K for lysis of bacterial cells and magnetic beads for DNA recovery. We optimized the method to consistently achieve sufficient yields of pure high‐molecular‐weight DNA with minimal reagents and time and developed a version of the protocol which can be performed without a centrifuge or electrical power. The suitability of the method was verified by MinION sequencing and assembly of 12 genomes of epidemiologically diverse fish‐pathogenic Streptococcus iniae and Streptococcus agalactiae isolates. The combination of effective high‐molecular‐weight DNA extraction and MinION sequencing enabled the discovery of a naturally occurring 15 kb low‐copy number mobilizable plasmid in S. iniae, which we name pSI1. We expect that our resource‐limited settings‐adapted protocol for high‐molecular‐weight DNA extraction could be implemented successfully for similarly recalcitrant‐to‐lysis gram‐positive bacteria, and it represents a method of choice for MinION‐based disease diagnostics in low‐ and middle‐income countries.
Autoimmune Neurobeachin Cerebellar Ataxia
Authors: Basal Eati , Gilligan Michael , Lesnick Connie , McKeon Andrew 
Published: August 2024

AbstractBackgroundThere are more than 30 immunoglobulin G (IgG) autoantibody biomarkers of autoimmune cerebellar ataxia reported.ObjectiveTo describe a novel IgG biomarker targeting neurobeachin, a scaffolding protein integral to synapse formation and function.MethodsClinical and laboratory‐based assessment was used for a novel diagnostic biomarker of autoimmune ataxia.ResultsA 46‐year‐old woman had ataxia onset followed by bilateral knee pain, attributable to rheumatoid arthritis. She was negative for standard neural antibodies. Her ataxia stabilized and tremor initially improved after corticosteroid treatment, but she progressed to wheelchair‐dependence (on methotrexate and then rituximab) over 4 years, in keeping with the trajectory encountered by many with autoimmune ataxia. In contrast, her inflammatory arthritis remitted with immunotherapy. Neurobeachin‐IgG was unmasked in serum and cerebrospinal fluid using protein arrays and confirmed using neurobeachin‐specific confocal immunofluorescence and Western blot.ConclusionsNeurobeachin‐IgG is a new neural antibody biomarker of cerebellar ataxia among a growing antibody repertoire, collectively common but often individually rare. © 2024 International Parkinson and Movement Disorder Society.