bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Sequence of the Petroleum Hydrocarbon-Degrading Bacterium
Alcanivorax
sp. Strain 97CO-5
Authors: Luan Xiao , Cui Zhisong , Gao Wei , Li Qian , Yin Xiaofei , Zheng Li 
Published: December 2014

ABSTRACT

Alcanivorax
sp. strain 97CO-5 was isolated from a crude-oil-degrading consortium, enriched from Yellow Sea sediment of China. Here, we present the draft genome of strain 97CO-5, which comprises 3,251,558 bp with a G+C content of 54.54% and contains 2,962 protein-coding genes and 42 tRNAs.
Draft Genome Sequence of a Xylanase-Producing Bacterial Strain, Cellvibrio mixtus J3-8
Authors: Wu Yi-Rui , Lin Bokun , Yu Yuli 
Published: December 2014

ABSTRACT

The xylanase-producing bacterial strain
Cellvibrio mixtus
J3-8 was isolated from grassland giant snails. The draft genome of strain J3-8 comprises 5,171,890 bp in 152 contigs with a G+C content of 46.66%. This is the first genome report about this bacterial species.
Draft Genome Sequence of Lactobacillus sakei Strain wikim 22, Isolated from Kimchi in Chungcheong Province, South Korea
Authors: Lim Hyeong In , Lee Jina , Jang Ja Young , Park Hae Woong , Choi Hak-Jong , Kim Tae-Woon , Kang Mi Ran , Lee Jong-Hee 
Published: December 2014

ABSTRACT

We report the draft genome sequence of
Lactobacillus sakei
strain wikim 22, a
Lactobacillus
species isolated from kimchi in North Chungcheong Province, South Korea, having 155 contigs with 2,447 genes and an average G+C content of 40.61%.
Draft Genome Sequence of Erwinia oleae, a Bacterium Associated with Olive Knots Caused by Pseudomonas savastanoi pv. savastanoi
Authors: Moretti Chiaraluce , Cortese Chiara , Passos da Silva Daniel , Venturi Vittorio , Firrao Giuseppe , Buonaurio Roberto 
Published: December 2014

ABSTRACT

Erwinia oleae
is a nonpathogenic bacterial species isolated from olive knots caused by
Pseudomonas savastanoi
pv. savastanoi. Since the presence of
E. oleae
in the knots increases disease severity, interspecies interactions with the pathogen are hypothesized. Here, we report the first draft genome sequence of the
E. oleae
type strain.
The development and production of a novel Smartphone App to collect day-to-day feedback from doctors-in-training and their trainers
Authors: Gray Thomas G , Hood Gill , Farrell Tom 
Published: December 2014

BackgroundFeedback drives learning in medical education. More regular multisource feedback would improve appraisal for doctors-in-training in the UK. Trainers currently receive little feedback on supervision they provide to doctors-in-training. Barriers against providing feedback include shortage of time and lack of clear mechanisms to do so.MethodsA literature review was undertaken to investigate whether a Smartphone App to provide feedback on training was a new concept. Different training environments were identified and feedback proformas for each of these areas developed for trainers and trainees. A pre-existing logbook App identified during literature searching was modified with permission and pilot phases completed. Professional software engineering was subsequently undertaken.ResultsThe App developed isHealthcare Supervision Logbook. Trainers can use it for providing feedback about a trainee's performance in a clinical session and trainees can use it for providing feedback about their perceived value of the educational content of a clinical session, including the educational and/or clinical supervision they have received.Healthcare Supervision Logbookalso contains a comprehensive logbook of practical procedures for all doctors using the App. It can also be used to collect patient and colleague feedback.ConclusionsHealthcare Supervision Logbookis a new concept. It allows trainers to provide daily feedback about a trainee's performance which can be used to facilitate appraisal and highlight areas for development. Feedback provided by the trainees can be used similarly to identify patterns and improve the quality of training provided. The feedback and logbook content is useful for purposes of appraisal and revalidation.
“A little on the heavy side”: a qualitative analysis of parents' and grandparents' perceptions of preschoolers' body weights
Authors: Eli Karin , Howell Kyndal , Fisher Philip A , Nowicka Paulina 
Published: December 2014

ObjectivesParents’ difficulties in perceiving children's weight status accurately pose a barrier for family-based obesity interventions; however, the factors underlying weight misinterpretation still need to be identified. This study's objective was to examine parents and grandparents’ perceptions of preschoolers’ body sizes. Interview questions also explored perceptions of parental responsibility for childhood obesity and appropriate contexts in which to discuss preschoolers’ weights.DesignSemistructured interviews, which were videotaped, transcribed and analysed qualitatively.SettingEugene and the Springfield metropolitan area, Oregon, USAParticipantsFamilies of children aged 3–5 years were recruited in February—May 2011 through advertisements about the study, published in the job seekers’ sections of a classified website (Craigslist) and in a local newspaper. 49 participants (22 parents and 27 grandparents, 70% women, 60% with overweight/obesity) from 16 low-income families of children aged 3–5 years (50% girls, 56% with overweight/obesity) were interviewed.ResultsThere are important gaps between clinical definitions and lay perceptions of childhood obesity. While parents and grandparents were aware of their preschoolers’ growth chart percentiles, these measures did not translate into recognition of children's overweight or obesity. The participants spoke of obesity as a problem that may affect the children in the future, but not at present. Participants identified childhood obesity as being transmitted from one generation to the next, and stigmatised it as resulting from ‘lazy’ parenting. Parents and grandparents avoided discussing the children's weights with each other and with the children themselves.ConclusionsThe results suggest that clinicians should clearly communicate with parents and grandparents about the meaning and appearance of obesity in early childhood, as well as counteract the social stigma attached to obesity, in order to improve the effectiveness of family-based interventions to manage obesity in early childhood.
CD99 Triggering in Ewing Sarcoma Delivers a Lethal Signal through p53 Pathway Reactivation and Cooperates with Doxorubicin
Authors: Guerzoni Clara , Fiori Valentina , Terracciano Mario , Manara Maria Cristina , Moricoli Diego , Pasello Michela , Sciandra Marika , Nicoletti Giordano , Gellini Mara , Dominici Sabrina , Chiodoni Claudia , Fornasari Pier Maria , Lollini Pier-Luigi , Colombo Mario P. , Picci Piero , Cianfriglia Maurizio , Magnani Mauro , Scotlandi Katia 
Published: December 2014

AbstractPurpose: The paucity of new drugs for the treatment of Ewing sarcoma (EWS) limits the cure of these patients. CD99 has a strong membranous expression in EWS cells and, being also necessary for tumor survival, is a suitable target to aim at. In this article, we described a novel human monospecific bivalent single-chain fragment variable diabody (dAbd C7) directed against CD99 of potential clinical application.Experimental Design: In vitro and in vivo evaluation of cell death and of the molecular mechanisms triggered by anti-CD99 agents were performed alone or in combination with doxorubicin to demonstrate efficacy and selectivity of the new dAbd C7.Results: The dAbd C7 induced rapid and massive EWS cell death through Mdm2 degradation and p53 reactivation. Mdm2 overexpression as well as silencing of p53 in p53wt EWS cells decreased CD99-induced EWS cell death, whereas treatment with nutlin-3 enhanced it. Furthermore, cell death was associated with induction of p21, bax, and mitochondrial depolarization together with substantial inhibition of tumor cell proliferation. Combined treatment of anti-CD99 dAbd C7 with doxorubicin was additive both in vitro and in vivo against EWS xenografts. Normal mesenchymal stem cells showed no p53 activation and were resistant to cell death, unless transformed by EWS-FLI, the oncogenic driver of EWS.Conclusions: These results indicate that dAbd C7 is a suitable candidate tool to target CD99 in patients with EWS able to spare normal stem cells from death as it needs an aberrant genetic context for the efficient delivery of CD99-triggered cell death. Clin Cancer Res; 21(1); 146–56. ©2014 AACR.
Activation of Vitamin D Receptor Signaling Downregulates the Expression of Nuclear FOXM1 Protein and Suppresses Pancreatic Cancer Cell Stemness
Authors: Li Zhiwei , Jia Zhiliang , Gao Yong , Xie Dacheng , Wei Daoyan , Cui Jiujie , Mishra Lopa , Huang Suyun , Zhang Yanqiao , Xie Keping 
Published: December 2014

AbstractPurpose: Dysregulated signaling of nuclear transcription factors vitamin D receptor (VDR) and Forkhead box M1 (FOXM1) plays important roles in transformation and tumorigenesis. In this study, we sought to determine whether VDR signaling causally affected FOXM1 signaling in and pathogenesis of pancreatic ductal adenocarcinoma (PDAC).Experimental Design: Genetic and pharmacologic approaches were used to manipulate VDR signaling. The impacts of altered VDR signaling on FOXM1 expression and function in PDAC cells were determined using molecular and biochemical methods, whereas those on PDAC cell biology and tumorigenicity were determined using in vitro and in vivo experimental systems. The clinical relevance of our findings was validated by analyzing human PDAC specimens.Results: There was a striking inverse correlation between reduced expression of VDR and increased expression of FOXM1 in human PDAC cells and tissues. Treatment of PDAC cells with 1,25-dihydroxyvitamin D3 (1,25D), its synthetic analogue EB1089 (EB), and VDR transgenics drastically inhibited FOXM1 signaling and markedly suppressed tumor stemness, growth, and metastasis. Mechanistically, 1,25D and EB repressed FOXM1 transcription and reduced the expression level of nuclear FOXM1 protein.Conclusion: Inactivation of Vitamin D/VDR signaling is a critical contributor to PDAC development and progression via elevated expression and function of FOXM1 and enhanced PDAC cell stemness, invasion, and metastasis. Clin Cancer Res; 21(4); 844–53. ©2014 AACR.
Resistive and Pulsatile Arterial Hemodynamics and Cardiovascular Events: The Multiethnic Study of Atherosclerosis
Authors: Lilly Scott M. , Jacobs David , Bluemke David A. , Duprez Daniel , Zamani Payman , Chirinos Julio 
Published: December 2014

Background

Arterial blood pressure is dependent on interactions between the heart and arteries. Resistive and pulsatile components of arterial load can be assessed by systemic vascular resistance (
SVR
, a microvascular property) and the ratio of stroke volume to pulse pressure (a surrogate of total arterial compliance,
TAC
), respectively. The relationship between arterial function and cardiovascular events in populations without cardiovascular disease is unknown.

Methods and Results

We studied 4806 adults enrolled in the Multi‐Ethnic Study of Atherosclerosis who were free of clinical cardiovascular disease at baseline.
SVR
and stroke volume/pulse pressure (
SV
/
PP
) were derived by sphygmomanometry and magnetic resonance imaging. The relationship between these measures of arterial function and incident cardiovascular events was assessed using Cox regression. With a mean follow‐up of 7.5 years, cardiovascular events occurred in 358 participants (7.4%). There was no relationship between
SVR
and subsequent cardiovascular events. However, increased stroke volume/pulse pressure was associated with reduced event rate in unadjusted (hazard ratio=0.67, 95%
CI
=0.58 to 0.77,
P
<0.001) and analyses that adjusted for multiple confounders (
HR
=0.75; 95%
CI
=0.62 to 0.90;
P
<0.001).

Conclusions

Greater total arterial compliance, manifest by higher stroke volume/pulse pressure is associated with a reduced incidence of subsequent
CVE
. In contrast,
SVR
was not independently associated with
CVE
in subjects free of overt cardiovascular disease at baseline. These findings support the concept that alternations in the large conduit vessels, rather than changes in microvascular resistance, are primarily related to incident cardiovascular disease.
Phenotypic Analysis of Arrhythmogenic Cardiomyopathy in the Hutterite Population: Role of Electrocardiogram in Identifying High‐Risk Desmocollin‐2 Carriers
Authors: Wong Jorge A. , Duff Henry J. , Yuen Tiffany , Kolman Louis , Exner Derek V. , Weeks Sarah G. , Gerull Brenda 
Published: December 2014

Background

The p.Gln554X mutation in desmocollin‐2 (
DSC
2) is prevalent in ≈10% of the Hutterite population. While the homozygous mutation causes severe biventricular arrhythmogenic right ventricular cardiomyopathy, the phenotypic features and prognosis of heterozygotes remain incompletely understood.

Methods and Results

Eleven homozygotes (mean age 32±8 years, 45% female), 28 heterozygotes (mean age 40±15 years, 50% female), and 22 mutation‐negatives (mean age 43±17 years, 41% female) were examined. Diagnostic testing was performed as per the arrhythmogenic right ventricular cardiomyopathy modified Task Force Criteria. Inverted T waves in the right precordial leads on
ECG
were seen in all homozygotes but not in their counterparts (
P
<0.001). Homozygotes had higher median daily
premature ventricular complex
burden than did heterozygotes or mutation‐negatives (1407 [
IQR
1080 to 2936] versus 2 [
IQR
0 to 6] versus 6 [
IQR
0 to 214],
P
=0.0002). Ventricular tachycardia was observed in 60% of homozygotes but in none of the remaining individuals (
P
<0.001). On cardiac magnetic resonance imaging, homozygotes had significantly larger indexed end‐diastolic volumes (right ventricular: 122±24 versus 83±17 versus 83±12 mL/m
2
,
P
<0.0001; left ventricular: 93±18 versus 76±13 versus 80±11 mL/m
2
,
P
=0.0124) and lower ejection fraction values compared with heterozygotes and mutation‐negatives (right ventricular ejection fraction: 41±9% versus 59±9% versus 61±6%,
P
<0.0001; left ventricular ejection fraction: 53±8% versus 65±5% versus 64±5%,
P
<0.0001). Most affected individuals lacked right ventricular wall motion abnormalities. Thus, few met cardiac magnetic resonance imaging task force criteria.

Conclusions

The
ECG
reliably identifies homozygous p.Gln554X carriers and may be useful as an initial step in the screening of high‐risk Hutterites. The cardiac phenotype of heterozygotes appears benign, but further prospective follow‐up of their arrhythmic risk is needed.
Clinical Prediction Algorithm (BRAIN) to Determine Risk of Hematoma Growth in Acute Intracerebral Hemorrhage
Authors: Wang Xia , Arima Hisatomi , Al-Shahi Salman Rustam , Woodward Mark , Heeley Emma , Stapf Christian , Lavados Pablo M. , Robinson Thompson , Huang Yining , Wang Jiguang , Delcourt Candice , Anderson Craig S. 
Published: December 2014

Background and Purpose—
We developed and validated a simple algorithm to predict the risk of hematoma growth in acute spontaneous intracerebral hemorrhage (ICH) to better inform clinicians and researchers in their efforts to improve outcomes for patients.

Methods—
We analyzed data from the computed tomography substudies of the pilot and main phases of the Intensive Blood Pressure Reduction in Acute Cerebral Hemorrhage Trials (INTERACT1 and 2, respectively). The study group was divided into a derivation cohort (INTERACT2, n=964) and a validation cohort (INTERACT1, n=346). Multivariable logistic regression was used to identify factors associated with clinically significant (≥6 mL) increase in hematoma volume at 24 hours after symptom onset. A parsimonious risk score was developed on the basis of regression coefficients derived from the logistic model.

Results—

A 24-point BRAIN score was derived from INTERACT2 (C-statistic, 0.73) based on baseline ICH volume (mL per score, ≤10=0, 10–20=5, >20=7), recurrent ICH (yes=4), anticoagulation with warfarin at symptom onset (yes=6), intraventricular extension (yes=2), and number of hours to baseline computed tomography from symptom onset (≤1=5, 1–2=4, 2–3=3, 3–4=2, 4–5=1, >5=0) predicted the probability of ICH growth (ranging from 3.4% for 0 point to 85.8% for 24 points) with good discrimination (C-statistic, 0.73) and calibration (Hosmer–Lemeshow
P
=0.82) in INTERACT1.

Conclusions—
The simple BRAIN score predicts the probability of hematoma growth in ICH. This could be used to improve risk stratification for research and clinical practice.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT00226096 and NCT00716079.
Optimal Excitation of Asymmetric Perturbations on an Axisymmetric Barotropic Vortex: A Linear Singular-Value Analysis
Authors: Itano Toshihisa 
Published: December 2014

Abstract
Singular vectors on a barotropic circular vortex consisting of three regions of piecewise-constant vorticity are investigated under the L2 norm to reveal the shape and growth rate of possible perturbations that may contribute to the formation of the hierarchal structure seen in natural and artificial vortices. Here, the analytical form of the singular values and the forward and backward singular vectors are derived for comparison with the corresponding eigenvalues and vectors. The results indicate no notable difference between the growing eigenvector and the singular vectors for Michalke and Timme’s profile, which is characterized by a ring of high vorticity. In contrast, for Syōno’s profile, which incorporates a negative vorticity region between a solidly rotating core and potentially rotating surroundings, the pattern of the forward singular vector giving the maximum growth in a specified time interval differs crucially from that of the growing eigenvector: whereas the radial peak of the streamfunction exists solely at the maximum-wind radius of the basic vortex in the growing eigenvector, it shifts outward in the growing forward singular vector. This implies the injection of considerable energy into the outer part of the basic vortex, rather than in the inner ring of the highest wind, facilitating the formation of hierarchal structure concentrated around the core region of the vortex.
Factors associated with surgical intervention in children with high-grade congenital hydronephrosis
Authors: Chalmers David J , Siomos Vassilis J , Gilsdorf Daniel J , Torok Michelle R , Vemulakonda Vijaya M 
Published: December 2014

Purpose: A lack of uniform guidelines regarding ureteropelvic junction obstruction may be associated with demographic variations in pyeloplasty timing. This study objective was to determine if children who undergo surgery are more likely to be of minority race. Materials and methods: Children with Society for Fetal Urology (SFU) 3–4 hydronephrosis were reviewed. Demographic and clinical characteristics were abstracted. Bivariable associations between clinical/demographic characteristics and pyeloplasty versus observation as well as follow-up findings were assessed with chi-square testing. The bivariable relationship between race and time from first clinic visit to surgery was evaluated by Kaplan-Meier curve. Multivariable logistic regression was performed to estimate the relationship between clinical characteristics and management decision. Results: Thirty-nine patients underwent pyeloplasty and 50 were observed. There were no differences in demographics, including race/ethnicity. Factors associated with surgery included higher SFU grade ( p = 0.01), preoperative MAG3 ( p = 0.02), and T½ >20 ( p = 0.01). On multivariate analysis, MAG3 T½ >20 was associated with surgery (OR = 0.20, 95% CI: 0.07–0.61). Median time to surgery for whites versus non-whites was not significant (232 vs 208 days, p = 0.16). Conclusions: In our cohort, the decision to perform pyeloplasty is associated with clinical rather than demographic characteristics. Despite controversy regarding T½ interpretation, delayed radiotracer clearance remained a significant predictor of surgery.
A Thousand Consecutive Treated Orthodontic Cases—A Survey
Authors: Rose J. S. 
Published: December 2014

An analysis of 1000 consecutive treated cases from a private orthodontic practice was made. Inter alia the results show: the male to female ratio was 4: 6; the mean age of first attendance was 10·7 years and the mean age at the start of active treatment was 11·6 years. 49 per cent of the cases were Angle Class I; 39 per cent Class II/1; 8 per cent Class II/2 and 4 per cent Class III. Over half the patients had an increased overbite. 21 per cent had an anterior crossbite and 11 per cent showed a posterior crossbite. Approximately 75 per cent of the cases showed crowding of the labial segments. There was crowding in 26 per cent of the upper buccal segments and in 49 per cent of the lower ones. 93 per cent of the patients received active treatment for the upper arch but only 4 per cent had appliances in the lower arch. A quarter of the cases were treated without upper extractions; in the lower arch the figure was 58 per cent. 94.1 per cent of the patients were treated with removable appliances requiring, on average, 1·5 appliances per case to complete treatment. 30 per cent of the cases received no retention. 54 per cent wore a retainer for less than 6 months. 88 per cent of the patients completed treatment and co-operation was satisfactory in 87 per cent. The mean treatment period for each patient was 13·1 months involving an average of 11·7 visits. The average active treatment time for each patients was 95 minutes. 74 per cent of the completed cases had a satisfactory result. The discussion supports the case for relating the type of orthodontic treatment to the total dental need of the patient. This requires more knowledge of what is meant by “dental health”. A plea is made that orthodontists should not become rigid in their approach to treatment.
Janus kinase inhibition by ruxolitinib extends dasatinib- and dexamethasone-induced remissions in a mouse model of Ph+ ALL
Authors: Appelmann Iris , Rillahan Cory D. , de Stanchina Elisa , Carbonetti Gregory , Chen Chong , Lowe Scott W. , Sherr Charles J. 
Published: December 2014

Key Points
In a Ph+ ALL mouse model, dasatinib inhibition of the BCR-ABL kinase resensitizes residual leukemic B cells to Janus kinase inhibition. Dasatinib, ruxolitinib, and dexamethasone together limit emergence of dasatinib-resistant BCR-ABL mutants and extend long-term survival.
Nebulised budesonide using a novel device in patients with oral steroid-dependent asthma
Authors: Vogelmeier Claus , Kardos Peter , Hofmann Thomas , Canisius Sebastian , Scheuch Gerhard , Muellinger Bernhard , Nocker Karlheinz , Menz Guenter , Rabe Klaus F. 
Published: December 2014

This phase 2/3 randomised, parallel-group, placebo-controlled trial investigated oral corticosteroid (OCS)-sparing efficacy, safety and tolerability of nebulised budesonide (Bud) administered with a novel computer-controlled, compressor-driven inhalation system (AKITA) as add-on therapy to Global Initiative for Asthma step 5.Patients (18–65 years) with OCS-dependent asthma were randomised (2:1:1:1) to receive 18-week, twice-daily, double-blind treatment with AKITA inhaled corticosteroid (AICS)-Bud 1 mg, AICS-Bud 0.5 mg, AICS-placebo or open-label Bud 1 mg administered by conventional nebuliser (CN-Bud). OCS doses were tapered until week 14.199 patients started treatment. More AICS-Bud 1 mg (80.0%) than placebo-treated (62.5%) patients had daily OCS doses reduced ≥50%, with clinical stability to week 18 (one-sided p=0.02; treatment difference: 17.5% (95% CI 0.1–34.9%), two-sided p=0.04). Mean±sdforced expiratory volume in 1 s improved (from baseline to week 18) for AICS-Bud 1 mg (239±460 mL, p<0.001) and AICS-Bud 0.5 mg (126±345 mL, p=0.01) but not placebo (93±419 mL, p=0.36) or CN-Bud (137±459 mL, p=0.18). Fewer AICS-Bud 1 mg-treated patients experienced asthma exacerbations (7.5%) compared with placebo (17.5%) or CN-Bud (22.5%). All treatments were well tolerated.Budesonide applied with AKITA allowed significant meaningful OCS reduction in OCS-dependent asthma patients while improving pulmonary function and maintaining exacerbation control.