bluuebunny/crossref_metadata_2025_split huggingface dataset:
The C-terminal Residues ofSaccharomyces cerevisiaeMec1 Are Required for Its Localization, Stability, and Function
Authors: DaSilva Lance F , Pillon Samantha , Genereaux Julie , Davey Megan J , Gloor Gregory B , Karagiannis Jim , Brandl Christopher J 
Published: October 2013

AbstractMec1, a member of the phosphoinositide three-kinase-related kinase (PIKK) family of proteins, is involved in the response to replicative stress and DNA damage and in telomere maintenance. An essential 30 to 35 residue, the FATC domain is found at the C-terminus of all PIKK family members. To investigate the roles of the C-terminal residues of Mec1, we characterized alleles of Saccharomyces cerevisiae mec1 that alter the FATC domain. A change of the terminal tryptophan to alanine resulted in temperature-sensitive growth, sensitivity to hydroxyurea, and diminished kinase activity in vitro. Addition of a terminal glycine or deletion of one, two, or three residues resulted in loss of cell viability and kinase function. Each of these Mec1 derivatives was less stable than wild-type Mec1, eluted abnormally from a size exclusion column, and showed reduced nuclear localization. We identified rpn3-L140P, which encodes a component of the 19S proteasomal regulatory particle of the 26S proteasome, as a suppressor of the temperature-sensitive growth caused by mec1-W2368A. The rpn3-L140P allele acted in a partially dominant fashion. It was not able to suppress the inviability of the C-terminal truncations or additions or the hydroxyurea sensitivity of mec1-W2368A. The rpn3-L140P allele restored Mec1-W2368A to nearly wild-type protein levels at 37°, an effect partially mimicked by the proteasome inhibitor MG-132. Our study supports a role for the C-terminus in Mec1 folding and stability, and suggests a role for the proteasome in regulating Mec1 levels.
Mono and Dual Cofactor Dependence of Human Cystathionine β-Synthase Enzyme Variants In Vivo and In Vitro
Authors: Dimster-Denk Dago , Tripp Katherine W , Marini Nicholas J , Marqusee Susan , Rine Jasper 
Published: October 2013

Abstract
Any two individuals differ from each other by an average of 3 million single-nucleotide polymorphisms. Some polymorphisms have a functional impact on cofactor-using enzymes and therefore represent points of possible therapeutic intervention through elevated-cofactor remediation. Because most known disease-causing mutations affect protein stability, we evaluated how the in vivo impact caused by single amino acid substitutions in a prototypical enzyme of this type compared with physical characteristics of the variant enzymes in vitro. We focused on cystathionine β-synthase (CBS) because of its clinical relevance in homocysteine metabolism and because some variants of the enzyme are clinically responsive to increased levels of its B6 cofactor. Single amino-acid substitutions throughout the CBS protein caused reduced function in vivo, and a subset of these altered sensitivity to limiting B6-cofactor. Some of these B6-sensitive substitutions also had altered sensitivity to limiting heme, another CBS cofactor. Limiting heme resulted in reduced incorporation of heme into these variants, and subsequently increased protease sensitivity of the enzyme in vitro. We hypothesize that these alleles caused a modest, yet significant, destabilization of the native state of the protein, and that the functional impact of the amino acid substitutions caused by these alleles can be influenced by cofactor(s) even when the affected amino acid is distant from the cofactor binding site.
Evaluating Sugarcane Families for Yield Potential and Repeatability Using Random Coefficient Models
Authors: Zhou M. M. , Kimbeng C.A. , Andru S. , Tew T. L. , Pontif M. J. , Gravois K. A. 
Published: October 2013

ABSTRACTSugarcane (Saccharum species hybrid) is a clonally propagated crop but during variety development, the first stage of selection occurs among seedlings established from true seed. All subsequent stages are established from vegetative stem cuttings and evaluated as clones. During family selection, whole families of seedlings are selected or rejected based on the family mean performance. Individual seedling selection is restricted to selected families. Confounding due to “seed type” can occur when the relative performance of families or individual genotypes differ between the seedling and clonal stages. The objective of this study was to demonstrate the use of random coefficient models (RCMs) for estimating the potential of sugarcane families to produce elite progeny. Data were collected from 17 families evaluated as seedlings in 2002 and clones in 2003. The elite families selected using RCM analysis produced higher mean cane yield in both the seedling and clonal stages and a higher proportion of high yielding clones than those selected using analysis of covariance and analysis of variance. The slope was the most discriminating parameter for family repeatability and was associated with high within‐family variability.
Identifying Mega‐Environments and Essential Test Locations for Pearl Millet Cultivar Selection in India
Authors: Gupta S. K. , Rathore A. , Yadav O. P. , Rai K. N. , Khairwal I. S. , Rajpurohit B. S. , Das R. R. 
Published: October 2013

ABSTRACTPearl millet [Pennisetum glaucum (L.) R. Br.] is grown under a wide range of environmental conditions in India. The All India Coordinated Pearl Millet Improvement Project (AICPMIP) has the responsibility of testing and releasing pearl millet cultivars adapted to such conditions. As a part of this process, AICPMIP has divided the entire pearl millet growing regions into three different zones (A1, A, and B) based on the rainfall pattern and local adaptation of the crop. This study was conducted to define the presently used test locations into possible mega‐environments and to identify essential test locations for cost‐effective evaluation of pearl millet cultivars. Grain yield data of different sets of 34 to 45 medium‐maturity pearl millet hybrids tested at 29 to 34 locations during 2006 to 2008 were analyzed using genotype main effects and genotype × environment interaction biplot method. Two distinct pearl millet mega‐environments with consistent grouping of locations across the years and corresponding to AICPMIP's designated A and B zones were identified. No such consistent grouping of locations corresponding to AICPMIP's designated A1 zone was, however, observed. Based on the discriminating ability, uniqueness, and research resources, 13 locations were identified as essential test locations for evaluation across the two mega‐environments. Testing at these locations appeared to provide good coverage of the whole pearl millet growing areas of India. Based on these findings, it is suggested to conduct initial yield trials at identified 13 locations across all the pearl millet growing zones represented by two mega‐environments followed by testing of selected hybrids with specific adaptation in their respective adaptation zones.
Incidence of Stroke According to Presence of Diabetic Nephropathy and Severe Diabetic Retinopathy in Patients With Type 1 Diabetes
Authors: Hägg Stefanie , Thorn Lena M. , Putaala Jukka , Liebkind Ron , Harjutsalo Valma , Forsblom Carol M. , Gordin Daniel , Tatlisumak Turgut , Groop Per-Henrik ,   on behalf of the FinnDiane Study Group
Published: October 2013

OBJECTIVE
Type 1 diabetes is associated with a markedly increased risk of stroke, but only a few studies on the incidence of stroke in type 1 diabetes exist. Therefore, we assessed the incidence of stroke in patients with type 1 diabetes and studied the impact of diabetic nephropathy (DN) and severe diabetic retinopathy (SDR) on this risk.

RESEARCH DESIGN AND METHODS
We studied 4,083 patients with type 1 diabetes from the Finnish Diabetic Nephropathy Study. Mean age was 37.4 ± 11.8 years, duration of diabetes was 21.6 ± 12.1 years, and 52% were men. Strokes were identified from medical records, death certificates, and the National Hospital Discharge Register and classified based on medical files and brain images.

RESULTS
During 36,680 person-years of follow-up, 149 (4%) patients suffered an incident stroke (105 infarctions and 44 hemorrhages). Of the infarctions, 58 (55%) were lacunar. The incidence of stroke, cerebral infarction, and cerebral hemorrhage was 406 (95% CI 344–477), 286 (234–347), and 120 (87–161) per 100,000 person-years, respectively. In an adjusted analysis, microalbuminuria increased the risk of stroke with a hazard ratio (HR) of 3.2 (1.9–5.6), macroalbuminuria 4.9 (2.9–8.2), and end-stage renal disease 7.5 (4.2–13.3), and SDR increased the risk with an HR of 3.0 (1.9–4.5). The risk of cerebral infarction, cerebral hemorrhage, and lacunar infarction increased in a similar manner. The proportion of lacunar versus nonlacunar infarction did not change across DN groups.

CONCLUSIONS
The presence of SDR and DN, independently, increases the risk of stroke, cerebral infarction, and cerebral hemorrhage in patients with type 1 diabetes.
Association of Obstructive Sleep Apnea in Rapid Eye Movement Sleep With Reduced Glycemic Control in Type 2 Diabetes: Therapeutic Implications
Authors: Grimaldi Daniela , Beccuti Guglielmo , Touma Carol , Van Cauter Eve , Mokhlesi Babak 
Published: October 2013

OBJECTIVE
Severity of obstructive sleep apnea (OSA) has been associated with poorer glycemic control in type 2 diabetes. It is not known whether obstructive events during rapid eye movement (REM) sleep have a different metabolic impact compared with those during non-REM (NREM) sleep. Treatment of OSA is often limited to the first half of the night, when NREM rather than REM sleep predominates. We aimed to quantify the impact of OSA in REM versus NREM sleep on hemoglobin A1c (HbA1c) in subjects with type 2 diabetes.

RESEARCH DESIGN AND METHODS
All participants underwent polysomnography, and glycemic control was assessed by HbA1c.

RESULTS
Our analytic cohort included 115 subjects (65 women; age 55.2 ± 9.8 years; BMI 34.5 ± 7.5 kg/m2). In a multivariate linear regression model, REM apnea–hypopnea index (AHI) was independently associated with increasing levels of HbA1c (P = 0.008). In contrast, NREM AHI was not associated with HbA1c (P = 0.762). The mean adjusted HbA1c increased from 6.3% in subjects in the lowest quartile of REM AHI to 7.3% in subjects in the highest quartile of REM AHI (P = 0.044 for linear trend). Our model predicts that 4 h of continuous positive airway pressure (CPAP) use would leave 60% of REM sleep untreated and would be associated with a decrease in HbA1c by approximately 0.25%. In contrast, 7 h of CPAP use would cover more than 85% of REM sleep and would be associated with a decrease in HbA1c by as much as 1%.

CONCLUSIONS
In type 2 diabetes, OSA during REM sleep may influence long-term glycemic control. The metabolic benefits of CPAP therapy may not be achieved with the typical adherence of 4 h per night.
Germination characteristics of Salicornia patula Duval-Jouve, S. emerici Duval-Jouve, and S. veneta Pign. et Lausi and their occurrence in Croatia
Authors: Šajna Nina , Regvar Marjana , Kaligarič Simona , Škvorc Željko , Kaligarič Mitja 
Published: October 2013

Abstract
- According to recent molecular analyses of Salicornia, we revised the annual glassworts from the Croatian coast, classified until now only as Salicornia europaea. Two species, a diploid Salicornia patula and a tetraploid S. emerici were recognized. They can be easily distinguished by floral characters, but not only by their habitus, which varies extremely according to environmental factors. Both species differ also in seed morphology. Salicornia patula has dimorphic seeds, with larger central seeds reaching high germination rates. Germination patterns helped to explain the habitat preferences. The species rarely co-occur, however. The rare S. patula occupies drier habitats, on coastal mudflats or sands that are irregularly inundated. It occurs within the assoc. Suaedo maritimae-Salicornietum patulae. Salicornia emerici occupies the lowest coastal mudflats, regularly inundated, where nutrient-rich conditions prevail, and forms an almost monotypical assoc. Salicornietum emerici. Due to the synonymy of S. veneta with S. emerici, we exclude the occurrence of S. veneta in Croatia as an independent taxon.
The Industrial Monuments Route of the Silesian Voivodeship as an example of the regional tourism product enhancing tourism competitiveness of the region
Authors: Staszewska Anna , Żemła Michał 
Published: October 2013

Abstract
The paper deals with the idea of destination competitiveness and its relations with competitiveness of spatial tourism products and competitiveness of entities catering tourism in the region. The case study method is used in the paper and the example of Industrial Monuments Route of the Silesian Province is analysed. The Route is a tourist product which presents the major and most interesting industrial facilities in the Silesian Region in terms of tourist, historic and architectonic values. The creation of the new spatial product - the Route by the regional authority - enhanced competitiveness of the Province as the image was improved, as well as a competitive advantage of operators of attractions within the Route was built. Some difficulties and challenges of such a way of enhancing destination competitiveness are discussed in the text.
Modelling of Postproduction Suspensions’ Concentration Processes by “Batch” Membrane Microfiltration
Authors: Szwast Maciej , Szwast Zbigniew , Grądkowski Marian , Piątkiewicz Wojciech 
Published: October 2013

Abstract
The mathematical model of postproduction suspension concentration by microfiltration has been developed. This model describes a process conducted in a batch system with membrane washing by reverse flow of permeate. The model considerations concern filtration pseudocycles consisting of the filtration period and the membrane washing period. The balances of continuous phase volume, dispersed phase mass and energy, for each period of pseudocycle respectively, have been presented.
High Pressure Vapour-Liquid Equilibrium of Volatiles in Supercritical Carbon Dioxide
Authors: Gurgenova Karina , Bogeł-Łukasik Rafał , Wawrzyniak Paweł 
Published: October 2013

Abstract
Binary vapour-liquid equilibrium of thymoquinone and carbon dioxide at the isothermal conditions was carried out at temperature 323.15 K and pressures from 6 to 10 MPa. The experimental data were fitted to the Soave-Redlich-Kwong equation of state. Results could be used for selection of process parameters in separation of volatiles from raw oil or for evaluation of existing separation technologies.
An Evaluation of the Levels of Vitamin D and Bone Turnover Markers After the Summer and Winter Periods in Polish Professional Soccer Players
Authors: Kopeć Aleksandra , Solarz Krzysztof , Majda Filip , Słowińska-Lisowska Małgorzata , Mędraś Marek 
Published: October 2013

Abstract
Vitamin D is synthesised in the skin during exposure to sunlight. The fundamental roles of vitamin D are the regulation of calcium and phosphate metabolism and bone mineralisation. Low vitamin D levels in athletes may adversely affect their exercise capabilities. The aim of our study was to investigate changes in serum levels of 25(OH)D3, calcium and bone turnover markers in football players in two training periods differing in the exposure to sunlight (after the summer period and after the winter period). We investigated 24 Polish professional soccer players. Serum levels of the following parameters were determined: 25(OH)D3, calcium, osteocalcin (OC), parathormone (PTH), procollagen type I N - terminal peptide (P1NP), and beta - CrossLaps (beta - CTx). We showed significantly higher levels of 25(OH)D3 and calcium and lower levels of PTH after the summer period versus the winter period. No significant differences in the levels of bone turnover markers were found. Furthermore, we did not observe any significant correlations between the levels of 25(OH)D3 and other parameters. Normal levels of 25(OH)D3 were observed in 50% of the players after the summer period and only in 16.7% of the players after the winter period. It is justified to measure the levels of 25(OH)D3, calcium and PTH in soccer players, especially after the winter period, when the exposure to sunlight is limited.
The Importance of Sports Performance Factors and Training Contents From the Perspective of Futsal Coaches
Authors: Serrano João , Shahidian Shakib , Sampaio Jaime , Leite Nuno 
Published: October 2013

Abstract
The aim of this study was to identify the importance assigned by futsal coaches with different education levels to the sports performance factors (technical, tactical, physical and psychological) and to the training contents. The sample was divided into three groups (novice: n=35, intermediate: n=42; and elite coaches: n=15) depending on the degree of specific education, coaching experience and the level of the teams trained. To achieve this goal, the coaches answered a questionnaire previously validated by specialists in sport sciences. The results showed significant differences between the novice and elite group in small-sided games, inferiority games, opposition and execution timing of the training and drill items. The analyses also showed significant differences between the novice and intermediate group in inferiority games and opposition of the training and drill items. Although, no differences were identified between groups for the remaining performance factors and training and drill items considered, the identified trends provide a baseline related to the knowledge that contributes to the development of expertise of futsal coaches.
Local abundance and spatial distribution of some migratory birds during post-breeding period
Authors: Gyurácz József , Kalmár Sándor , Baráth Réka 
Published: October 2013

AbstractThe local abundance and spatial distribution of the short- to medium-distance migratory and daytime stopover passerines (Robin Erithacus rubecula, Blackcap Sylvia atricapilla, Chiffchaff Phylloscopus collybita, Blue tit Parus caeruleus, Great tit P. major) were studied in a West Hungarian stopover ground during post-breeding season. The dispergation index of all migratory bird species revealed clumped distribution both in „smallest annual capture year” (abb. SACY) and the „largest annual capture year” (abb. LACY). According to the PCA the spatial occurrences of Blackcap, Blue tit and Great tit captured in LACY showed significantly higher concentration than of those migrating in SACY. The studied species appeared in all four habitats (bushy, forest, grassland, marsh) of the study stopover area, but their clumped spatial distribution showed habitat preference. The abundance-dependent shift of habitat selection was found only in Great tit, the most of them captured in SACY concentrated in grassland with bushy, while the ones captured in LACY grouped in forest habitat type. Blackcaps were grouped the grassland with bushes habitat type where many Dwarf elder (Sambucus ebulus) bushes were available during autumn migration.
Gdansk Bay sea bed sounding and classification of its results
Authors: Grelowska Grażyna , Kozaczka Eugeniusz , Kozaczka Sławomir , Szymczak Wojciech 
Published: October 2013

AbstractThe main goal of this paper is to describe the results of sounding the Gdansk Bay sea bed by using a SES- 2000 Standard parametric sub-bottom profiler. Quality of data obtained during trials depends inter alia on a proper location of transducer array to reduce influence of pitch, roll and heave motions as well as ship self noise (resulting from bubbles due to propeller and flow around hull, vibration generated by main engine and auxiliary devices). Furthermore, calibration of complementary units such as GPS, heading sensor, MRU-Z motion sensor and navigation devices make sea-bed investigating system capable of working with its whole capability. Results of so prepared surveys have been presented and discussed. They contain also an elaborated map of Gdansk Bay with preliminarily classified sea-bed materials and description of most interesting areas.
Some Remarks on the Choice of Ductility Class for Earthquake-Resistant Steel Structures
Authors: Matejčeková-Farhat Miroslava , Ároch Rudolf 
Published: October 2013

Abstract
The implementation of the Eurocodes in current structural design practice has brought about a new emphasis on the design of earthquake-resistant structures. In some European countries, new earthquake zones have been defined; henceforth, the design requirements of many ongoing projects have changed as well. The choice of the ductility class of steel structures as one of the key design parameters, the consequences of this choice on design procedure, and some applications of the Eurocode 8 design criteria by comparing French and Slovak national practice are discussed, using a practical example of a structure.
Silo with a Corrugated Sheet Wall
Authors: Németh Csaba , Brodniansky Ján 
Published: October 2013

Abstract
Silos and tanks are currently being used to create reserves of stored materials. Their importance is based on balancing the production and consumption of bulk materials to establish an adequate reserve throughout the year. The case study introduced within the framework of this paper focuses on thin-walled silos made of corrugated sheets and on an approach for designing these types of structures. The storage of bulk materials causes compression or tensile stresses in the walls of a silo structure. The effect of a frictional force in the silo walls creates an additional bending moment in a wave, which ultimately affects the resulting bending moments. Several mathematical and physical models were used in order to examine various types of loading and their effects on a structure. Subsequently, the accuracy of the computational models was verified by experimental measurements on a grain silo in Bojničky, Slovakia. A comparison of the experimental and mathematical models shows a reasonable match and confirms the load specifications, while indicating that the mathematical model was correct.
Augmentation of major isoflavones in Glycine max L. through the elicitor-mediated approach
Authors: Saini Ramesh K. , Akithadevi Muthu K. , Giridhar Parvatam , Ravishankar Gokare A. 
Published: October 2013

Abstract
- Isoflavone content in soybean seeds was enhanced by the elicitor-mediated approach under field conditions through the floral application of abiotic elicitors-salicylic acid, methyl jasmonate and biotic elicitors-Aspergillus niger and Rhizopus oligosporus. Among isoflavones, daidzein and glycitein were found to be highly responsive to elicitors, with an increase of 53.7% and 78.7%, respectively as compared to control. Highest total isoflavone content (1276.4 mg g-1 of seeds) was observed upon the administration of 0.1 mMsalicylic acid, which is 92.7% higher than in control. This study would be valuable for augmentation of the isoflavone content in soybean seeds in field grown plants for better nutraceutical potential.