bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lysine-specific demethylase 1-mediated demethylation of histone H3 lysine 9 contributes to interleukin 1β-induced microsomal prostaglandin E synthase 1 expression in human osteoarthritic chondrocytes
Authors: El Mansouri Fatima Ezzahra , Nebbaki Salwa-Sarah , Kapoor Mohit , Afif Hassan , Martel-Pelletier Johanne , Pelletier Jean-Pierre , Benderdour Mohamed , Fahmi Hassan 
Published: May 2014

Abstract
Introduction
Microsomal prostaglandin E synthase 1 (mPGES-1) catalyzes the terminal step in the biosynthesis of PGE2, a critical mediator in the pathophysiology of osteoarthritis (OA). Histone methylation plays an important role in epigenetic gene regulation. In this study, we investigated the roles of histone H3 lysine 9 (H3K9) methylation in interleukin 1β (IL-1β)-induced mPGES-1 expression in human chondrocytes.

Methods
Chondrocytes were stimulated with IL-1β, and the expression of mPGES-1 mRNA was evaluated using real-time RT-PCR. H3K9 methylation and the recruitment of the histone demethylase lysine-specific demethylase 1 (LSD1) to the mPGES-1 promoter were evaluated using chromatin immunoprecipitation assays. The role of LSD1 was further evaluated using the pharmacological inhibitors tranylcypromine and pargyline and small interfering RNA (siRNA)-mediated gene silencing. The LSD1 level in cartilage was determined by RT-PCR and immunohistochemistry.

Results
The induction of mPGES-1 expression by IL-1β correlated with decreased levels of mono- and dimethylated H3K9 at the mPGES-1 promoter. These changes were concomitant with the recruitment of the histone demethylase LSD1. Treatment with tranylcypromine and pargyline, which are potent inhibitors of LSD1, prevented IL-1β-induced H3K9 demethylation at the mPGES-1 promoter and expression of mPGES-1. Consistently, LSD1 gene silencing with siRNA prevented IL-1β-induced H3K9 demethylation and mPGES-1 expression, suggesting that LSD1 mediates IL-1β-induced mPGES-1 expression via H3K9 demethylation. We show that the level of LSD1 was elevated in OA compared to normal cartilage.

Conclusion
These results indicate that H3K9 demethylation by LSD1 contributes to IL-1β-induced mPGES-1 expression and suggest that this pathway could be a potential target for pharmacological intervention in the treatment of OA and possibly other arthritic conditions.
Intergenerational genomic DNA methylation patterns in mouse hybrid strains
Authors: Orozco Luz D , Rubbi Liudmilla , Martin Lisa J , Fang Fang , Hormozdiari Farhad , Che Nam , Smith Andrew D , Lusis Aldons J , Pellegrini Matteo 
Published: May 2014

Abstract
Background
DNA methylation is a contributing factor to both rare and common human diseases, and plays a major role in development and gene silencing. While the variation of DNA methylation among individuals has been partially characterized, the degree to which methylation patterns are preserved across generations is still poorly understood. To determine the extent of methylation differences between two generations of mice we examined DNA methylation patterns in the livers of eight parental and F1 mice from C57BL/6J and DBA/2J mouse strains using bisulfite sequencing.

Results
We find a large proportion of reproducible methylation differences between C57BL/6J and DBA/2J chromosomes in CpGs, which are highly heritable between parent and F1 mice. We also find sex differences in methylation levels in 396 genes, and 11% of these are differentially expressed between females and males. Using a recently developed approach to identify allelically methylated regions independently of genotypic differences, we identify 112 novel putative imprinted genes and microRNAs, and validate imprinting at the RNA level in 10 of these genes.

Conclusions
The majority of DNA methylation differences among individuals are associated with genetic differences, and a much smaller proportion of these epigenetic differences are due to sex, imprinting or stochastic intergenerational effects. Epigenetic differences can be a determining factor in heritable traits and should be considered in association studies for molecular and clinical traits, as we observed that methylation differences in the mouse model are highly heritable and can have functional consequences on molecular traits such as gene expression.
Do We Now Know What Inappropriate Laboratory Utilization Is?
Authors: Hauser Ronald G. , Shirts Brian H. 
Published: May 2014

Abstract

Objectives:
Many nonpathologists and some pathologists consider utilization review essential to laboratory quality improvement, but (1) confusion surrounding the definition of “appropriate” laboratory utilization, (2) the reliance on manual chart review, and (3) a lack of leadership have contributed to its unstandardized implementation. How the solutions to these barriers have evolved since the 1950s is described.

Methods:
A systematic literature review is used.

Results:
Current literature largely defines inappropriate laboratory utilization as any test order in violation of a guideline produced by a government or professional society. Audits performed without manual chart review (ie, database query) have dramatically increased since the mid-1990s. Most utilization audits do not involve any author with a pathology or laboratory medicine affiliation.

Conclusions:
Literature consensus defining “inappropriate” utilization combined with the adoption of database technology has removed key obstacles to utilization reviews. Leadership is needed to unify and benchmark laboratory utilization.
Sustainability, new economics and policy: Greening pathway for the auto industry
Authors: Hwang Kwangseon 
Published: May 2014

Abstract
There have been several arguments from scholars on the question of how we can attain sustainable development (SD). The aim of the article is to explore realistic policies and programmes for achieving sustainable auto manufacturing with a question of ‘how do we manufacture vehicles in a way that is sustainable?’ To discuss how we can achieve sustainable auto manufacturing, I draw on a framework consisting of four innovations–technological, organizational, institutional and social change–and three sustainability pillars–economic development, the environment and employment. All four kinds of innovation need to receive attention in a coordinated manner and careful government policies are needed, thereby reaching a certain sustainability goal.
Singularities on the base of a Fano type fibration
Authors: Birkar Caucher 
Published: May 2014

Abstract

Let

f
:
X
→
Z

${f\colon X\rightarrow Z}$

be a Mori fibre space. McKernan conjectured that the
singularities of Z are bounded in terms of the singularities of X. Shokurov independently
proposed a more general conjecture in the
setting of pairs: let (X,B) be a pair with klt singularities
and

f
:
X
→
Z

${f\colon X\rightarrow Z}$

be a contraction such that

K
X

+
B

∼
ℝ

0
/
Z

$K_X+B\sim _\mathbb {R}0/Z$

and
that the general fibres of f are Fano type varieties; adjunction for fibre spaces produces a
discriminant divisor BZ
and a moduli divisor MZ
on Z.
It is then conjectured that the singularities of

(
Z
,

B
Z

+

M
Z

)

${(Z,B_Z+M_Z)}$

are bounded in terms of the
singularities of (X,B). We prove Shokurov's conjecture when

(
F
,
Supp

B
F

)

${(F,\operatorname{Supp}B_F)}$

belongs
to a bounded family where F is a general fibre of f and

K
F

+

B
F

=

(

K
X

+
B
)

|

F

${K_F+B_F=(K_X+B)|_F}$

.
Slow relaxation mode of sodium lignosulfonate in saline solutions
Authors: Qian Yong , Deng Yonghong , Qiu Xueqing , Lou Hongming , Pang Yuxia 
Published: May 2014

Abstract
Sodium lignosulfonate (NaLS) was dissolved in saline solutions, and the dissolving process was examined by a combination of static and dynamic light scattering. When NaLS was dissolved in 1.2 mol l-1 NaCl solution for 24 h and filtered through a 0.45 μm syringe filter, only a fast relaxation mode was observed, which was attributed to the co-diffusion of NaLS molecular ions and counterions. When the concentration of NaCl was below 1.2 mol l-1 or the dissolving time was <24 h, an additional slow relaxation mode (SRM) appeared due to hindered motions of electrostatically interacting chains. Without filtering or filtering followed by rising temperature to 38°C, SRM appeared due to aggregation, which occurred at a position similar to that caused by electrostatic interaction. For poly(sodium styrenesulfonate) (NaPSS) and modified bamboo lignin derivate with open polyelectrolyte structures, 0.1 mol l-1 NaCl was enough to inhibit the electrostatic interaction. For NaLS with a closed polyelectrolyte structure, the SRM could not be removed until the concentration of NaCl reached 1.2 mol l-1. Above observations give insights for the first time into the relationship between the SRM caused by electrostatically interacting chains and the diffusion mode due to NaLS aggregation, and the influence of the closed polyelectrolyte structure on the SRM.
Assessment of Quality Attributes of Banana Slices Dried by Different Drying Methods
Authors: Pirnazari Kianoosh , Esehaghbeygi Ali , Sadeghi Morteza 
Published: May 2014

Abstract
In the present study, banana slices were dried and compared using electrohydrodynamic (EHD) drying method at 4.5, 6.5, and 8.5 kV/cm; oven at 50, 60, and 70°C; and hot-air drying at 50, 60, and 70°C at a constant air velocity of 1.5 m/s. ANOVA showed that drying method had a significant effect on drying time, rehydration capacity, shrinkage, color features (L*, a*, b*, and ∆E), and consumed energy. The values of consuming energy for EHD at 8.5 kV/cm, oven, and hot-air drying at 70°C were 2.99, 20.9, and 81.7 kJ/g run on full capacity of each dryer, respectively. Oven drying led to a greater color change than did hot-air. No significant difference was observed between the color change feature ∆E for EHD and hot-air dried samples in the orthogonal contrast analysis. The falling rate period for EHD moisture movement showed that diffusion was describing the moisture gradients and the internal moisture transfer was dominant. EHD is not fast; however, its advantages like less energy needed, lower shrinkage, and great rehydration capacity make it a good choice for combining with other convection drying methods.
Inverse free boundary problems for a generally degenerate parabolic equation
Authors: Huzyk Nadiya 
Published: May 2014

Abstract
In a free boundary domain we consider inverse problems for determination
a time-dependent coefficient at the first derivative of an unknown function in a generally
degenerate parabolic equation with different overdetermination conditions. There are established conditions of existence and
uniqueness of the classical solutions to the named problems in the case of the weak degeneration.
Racial/ethnic differences in the birth prevalence of congenital anomalies in the United States
Authors: Egbe Alexander , Lee Simon , Ho Deborah , Uppu Santosh , Srivastava Shubhika 
Published: May 2014

Abstract

Objectives: This work aimed to analyze a single large representative population to determine racial/ethnic differences in the prevalence of congenital anomalies (CA).

Design: This is a population-based comprehensive analysis of racial/ethnic differences in the prevalence of CA diagnoses. We reviewed all live births in the 2008 Nationwide Inpatient Sample (NIS) database and determined the birth prevalence of 55 selected CA diagnoses in Caucasians. We then calculated the relative risk of these CA diagnoses in African American, Hispanics, and Asians relative to Caucasians.

Result: Overall CA prevalence was 29.2 per 1000 in a cohort of 1,048,252 live births, of which 51% were Caucasians. Compared with Caucasians, risk of overall CA was lower in African Americans (RR=0.9, CI: 0.8–0.9) and Hispanics (RR=0.9, CI: 0.8–0.9). Risk of overall CA was similar in Caucasians and Asians. Relative to the Caucasians, African Americans had lower risk of cardiac, genitourinary, and craniofacial CA but higher risk of musculoskeletal anomalies. Hispanics had lower risk of genitourinary and gastrointestinal CA. Asians had higher risk of craniofacial and musculoskeletal CA.

Conclusions: Observed racial/ethnic differences in risk of CA may be related to genetic susceptibilities, cultural or social differences that could modify exposures, or many potential combinations between susceptibilities and exposures.
S100A1 is released from ischemic cardiomyocytes and signals myocardial damage via Toll‐like receptor 4
Authors: Rohde David , Schön Christoph , Boerries Melanie , Didrihsone Ieva , Ritterhoff Julia , Kubatzky Katharina F , Völkers Mirko , Herzog Nicole , Mähler Mona , Tsoporis James N , Parker Thomas G , Linke Björn , Giannitsis Evangelos , Gao Erhe , Peppel Karsten , Katus Hugo A , Most Patrick 
Published: May 2014

AbstractMembers of the S100 protein family have been reported to function as endogenous danger signals (alarmins) playing an active role in tissue inflammation and repair when released from necrotic cells. Here, we investigated the role of S100A1, the S100 isoform with highest abundance in cardiomyocytes, when released from damaged cardiomyocytes during myocardial infarction (MI). Patients with acute MI showed significantly increased S100A1 serum levels. Experimental MI in mice induced comparable S100A1 release. S100A1 internalization was observed in cardiac fibroblasts (CFs) adjacent to damaged cardiomyocytes. In vitro analyses revealed exclusive S100A1 endocytosis by CFs, followed by Toll‐like receptor 4 (TLR4)‐dependent activation of MAP kinases and NF‐κB. CFs exposed to S100A1 assumed an immunomodulatory and anti‐fibrotic phenotype characterized i.e. by enhanced intercellular adhesion molecule‐1 (ICAM1) and decreased collagen levels. In mice, intracardiac S100A1 injection recapitulated these transcriptional changes. Moreover, antibody‐mediated neutralization of S100A1 enlarged infarct size and worsened left ventricular functional performance post‐MI. Our study demonstrates alarmin properties for S100A1 from necrotic cardiomyocytes. However, the potentially beneficial role of extracellular S100A1 in MI‐related inflammation and repair warrants further investigation.
Bypassing the Requirement for an Essential MYST Acetyltransferase
Authors: Torres-Machorro Ana Lilia , Pillus Lorraine 
Published: May 2014

Abstract
Histone acetylation is a key regulatory feature for chromatin that is established by opposing enzymatic activities of lysine acetyltransferases (KATs/HATs) and deacetylases (KDACs/HDACs). Esa1, like its human homolog Tip60, is an essential MYST family enzyme that acetylates histones H4 and H2A and other nonhistone substrates. Here we report that the essential requirement for ESA1 in Saccharomyces cerevisiae can be bypassed upon loss of Sds3, a noncatalytic subunit of the Rpd3L deacetylase complex. By studying the esa1∆ sds3∆ strain, we conclude that the essential function of Esa1 is in promoting the cellular balance of acetylation. We demonstrate this by fine-tuning acetylation through modulation of HDACs and the histone tails themselves. Functional interactions between Esa1 and HDACs of class I, class II, and the Sirtuin family define specific roles of these opposing activities in cellular viability, fitness, and response to stress. The fact that both increased and decreased expression of the ESA1 homolog TIP60 has cancer associations in humans underscores just how important the balance of its activity is likely to be for human well-being.
Structural features and dopant gradients in Mg2SnXSi1-X ternary compounds
Authors: Hatzikraniotis E. , Polymeris G.S. , Lioutas C.B. , Burkov A. , Stefanaki E-C. , Samunin A. , Isachenko G. , Fedorov M.I. , Paraskevopoulos K.M. 
Published: May 2014

ABSTRACTIn the present work, a comparative study is attempted, dealing with the influence of the grain size distribution on the microstructure and the free carrier concentration in Mg2SnXSi1-X (x=0.2) ternary compounds doped with Sb. Structural in-homogeneities were monitored by using Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM) as well as Fourier transform infrared spectroscopy (FTIR) in the reflectivity mode.
Clinical Outcomes Following Regenerative Therapy of Non‐Contained Intrabony Defects Using a Deproteinized Bovine Bone Mineral Combined With Either Enamel Matrix Derivative or Collagen Membrane
Authors: Iorio‐Siciliano Vincenzo , Andreuccetti Gianmaria , Blasi Andrea , Matarasso Marco , Sculean Anton , Salvi Giovanni E. 
Published: May 2014

Background: The purpose of this study is to compare clinical outcomes in the treatment of deep non‐contained intrabony defects (i.e., with ≥70% 1‐wall component and a residual 2‐ to 3‐wall component in the most apical part) using deproteinized bovine bone mineral (DBBM) combined with either enamel matrix protein derivative (EMD) or collagen membrane (CM).Methods: Forty patients with multiple intrabony defects were enrolled. Only one non‐contained defect per patient with an intrabony depth ≥3 mm located in the interproximal area of single‐ and multirooted teeth was randomly assigned to the treatment with either EMD + DBBM (test: n = 20) or CM + DBBM (control: n = 20). At baseline and after 12 months, clinical parameters including probing depth (PD) and clinical attachment level (CAL) were recorded. The primary outcome variable was the change in CAL between baseline and 12 months.Results: At baseline, the intrabony component of the defects amounted to 6.1 ± 1.9 mm for EMD + DBBM and 6.0 ± 1.9 mm for CM + DBBM sites (P = 0.81). The mean CAL gain at sites treated with EMD + DBBM was not statistically significantly different (P = 0.82) compared with CM + DBBM (3.8 ± 1.5 versus 3.7 ± 1.2 mm). No statistically significant difference (P = 0.62) was observed comparing the frequency of CAL gain ≥4 mm between EMD + DBBM (60%) and CM + DBBM (50%) or comparing the frequency of residual PD ≥6 mm between EMD + DBBM (5%) and CM + DBBM (15%) (P = 0.21).Conclusion: Within the limitations of the present study, regenerative therapy using either EMD + DBBM or CM + DBBM yielded comparable clinical outcomes in deep non‐contained intrabony defects after 12 months.
Evaluation of Efficacy of Chlorhexidine Intracanal Medicament on the Periodontal Healing of Concomitant Endodontic‐Periodontal Lesions Without Communication: An Interventional Study
Authors: Raheja Jyoti , Tewari Sanjay , Tewari Shikha , Duhan Jigyasa 
Published: May 2014

Background: Treatment of concomitant endodontic‐periodontal lesions remains a challenge in clinical practice and requires effective endodontic and regenerative periodontal therapy. Among other factors, cross seeding and recolonization of flora may affect the outcome of periodontal therapy. Intracanal medicaments have been shown to exert antimicrobial activity on the external root surface, and local delivery of antimicrobials has been suggested to be a complementary approach in the management of periodontitis. Therefore, the objective of this study is to determine the influence of chlorhexidine (CHX) intracanal medicament on the clinical outcomes of therapy.Methods: Thirty‐one patients were divided into two treatment groups: 1) open flap debridement (OFD) in endodontically treated teeth (control); and 2) OFD in endodontically treated teeth with CHX placed in the coronal space (test). The clinical variables evaluated were probing depth (PD), clinical attachment level (CAL), and percentage of sites with PD ≥5 mm. Reevaluation was performed at 3 and 6 months post‐surgery.Results: Both treatments resulted in improvement in all the clinical variables evaluated. Postoperative measurements from test and control groups showed reductions in mean PD of 2.22 ± 1.27 and 0.91 ± 0.81 mm, mean CAL gains of 2.16 ± 1.12 and 0.60 ± 0.93 mm, and 43.33% ± 31.37% and 17.71% ± 14.23% reduction in sites with PD ≥5 mm. Significantly more PD reduction, CAL gain, and percentage reduction in sites with PD ≥5 mm were observed in the test group at 6 months (P <0.05).Conclusion: CHX may be used as an effective intracanal medicament for promoting periodontal healing in concomitant endodontic‐periodontal lesions.
Short‐Term Effects of Non‐Surgical Periodontal Treatment on the Gingival Crevicular Fluid Cytokine Profiles in Sites With Induced Periodontal Defects: A Study on Dogs With and Without Streptozotocin‐Induced Diabetes
Authors: Javed Fawad , Al‐Daghri Nasser M. , Wang Hom‐Lay , Wang Cun Yu , Al‐Hezaimi Khalid 
Published: May 2014

Background: The aim of this study is to assess the short‐term effects of non‐surgical periodontal therapy (NSPT) on the gingival crevicular fluid (GCF) cytokine profile in sites with standardized periodontal bony defects in beagle dogs with and without diabetes.Methods: Four beagle dogs with streptozotocin (STZ)‐induced diabetes and four healthy dogs were included. Fasting blood glucose levels were measured using a glucometer. In all animals, a 3‐walled bony defect was created on the mesial surface of the second premolar and first molar in all quadrants. After 12 weeks, all animals underwent weekly NSPT for 3 weeks. Baseline and post‐NSPT GCF samples were collected, and levels of interleukin (IL)‐1, IL‐1β, IL‐6, IL‐8, and tumor necrosis factor (TNF)‐α were measured using enzyme‐linked immunosorbent assay. Statistical analyses were performed using a software program, and P values <0.05 were considered statistically significant.Results: Mean fasting blood glucose levels were significantly higher in dogs with induced diabetes than those without diabetes (P <0.01). At baseline, mean IL‐6 (P <0.01) and IL‐8 (P <0.05) levels were higher in dogs with diabetes than those without diabetes. A significant reduction in levels of IL‐1, IL‐1β, IL‐6, IL‐8, and TNF‐α was noted in dogs without diabetes 1 week after NSPT. However, this significant reduction (P <0.05) only appeared 2 weeks after NSPT in dogs with diabetes.Conclusions: NSPT reduces GCF levels of proinflammatory cytokines in dogs with and without STZ‐induced diabetes; however, chronic hyperglycemia seems to retard the effect of NSPT on GCF cytokine concentration.
On the Temporal Fidelity of Nonlinear Inverse Reconstructions for Real- Time MRI – The Motion Challenge
Authors: Frahm Jens , Schätz Sebastian , Untenberger Markus , Zhang Shuo , Voit Dirk , Merboldt K. Dietmar , Sohns Jan M. , Lotz Joachim , Uecker Martin 
Published: May 2014

Purpose:
To evaluate the temporal accuracy of a self-consistent nonlinear inverse reconstruction method
(NLINV) for real-time MRI using highly undersampled radial gradient-echo sequences and to present an open source
framework for the motion assessment of real-time MRI methods.

Methods:
Serial image reconstructions by NLINV combine a joint estimation of individual frames and corresponding coil
sensitivities with temporal regularization to a preceding frame. The temporal fidelity of the method was determined with a
phantom consisting of water-filled tubes rotating at defined angular velocity. The conditions tested correspond to real-time
cardiac MRI using SSFP contrast at 1.5 T (40 ms resolution) and T1 contrast at 3.0 T (33 ms and 18 ms resolution).
In addition, the performance of a post-processing temporal median filter was evaluated.

Results:
NLINV reconstructions without temporal filtering yield accurate estimations as long as the speed of a small
moving object corresponds to a spatial displacement during the acquisition of a single frame which is smaller than the
object itself. Faster movements may lead to geometric distortions. For small objects moving at high velocity, a median
filter may severely compromise the spatiotemporal accuracy.

Conclusion:
NLINV reconstructions offer excellent temporal fidelity as long as the image acquisition time is short enough
to adequately sample (“freeze”) the object movement. Temporal filtering should be applied with caution. The motion
framework emerges as a valuable tool for the evaluation of real-time MRI methods.