bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of Chondrocyte and Synovial Cell Death After Exposure to Commonly Used Anesthetics
Authors: Rao Allison J. , Johnston Tyler R. , Harris Alex H.S. , Smith R. Lane , Costouros John G. 
Published: October 2013

Background: An intra-articular injection of local anesthetics is a common procedure for diagnostic and therapeutic purposes. It has been shown that these agents are toxic to articular cartilage and synovial tissue in a dose- and time-dependent fashion, and in some cases, they may lead to postarthroscopic glenohumeral chondrolysis (PAGCL). However, the role of apoptosis in cell death is still unclear, and the potential role of apoptosis inhibition in minimizing chondrocyte and synovial cell death has not been reported. Purpose: (1) To quantify the degree of apoptotic cell death in chondrocytes and synovial cells exposed to local anesthetics, and (2) to determine whether caspase inhibition could reduce cell death. Study Design: Controlled laboratory study. Methods: Human chondrocytes and synovial cells were expanded in vitro and exposed to normal saline, 0.5% bupivacaine, 0.5% ropivacaine, 1% lidocaine, or 1:1000 epinephrine for 90 minutes. Apoptosis was then detected at 1, 3, 5, and 7 days after exposure using terminal deoxynucleotidyl transferase (TdT)–mediated dUTP nick-end labeling (TUNEL) and immunohistochemistry. Apoptosis was then inhibited using the pan-caspase inhibitor z-vad- fmk. Results were normalized to normal saline controls and analyzed by generalized regression models and pairwise confidence intervals. Results: Analysis of cumulative chondrocyte apoptosis relative to controls after anesthetic exposure demonstrated more than 60% cell death with 0.5% bupivacaine and 1:1000 epinephrine. The greatest chondroprotective effect of caspase inhibition occurred with 0.5% ropivacaine. Similarly, in synovial cells, epinephrine was also very cytotoxic; however, 1% lidocaine induced the most apoptosis. Synovial cells exposed to 0.5% ropivacaine were again most sensitive to protective caspase inhibition. Conclusion: Local anesthetics induce chondrocyte and synovial cell apoptosis in a time-dependent fashion, with peak apoptosis occurring 5 days after exposure. Both chondrocytes and synovial cells are most sensitive to caspase inhibition after exposure to 0.5% ropivacaine. Clinical Relevance: Apoptosis inhibition may be an effective strategy in minimizing chondrocyte and synovial cell death after exposure to anesthetics. Further investigation is clinically warranted.
Maternal and Breast Pump Factors Associated with Breast Pump Problems and Injuries
Authors: Qi Youlin , Zhang Yuanting , Fein Sara , Wang Cunlin , Loyo-Berríos Nilsa 
Published: October 2013

Background: Benefits of using a breast pump are well documented, but pump-related problems and injuries and the associated risk factors have not been reported. Objectives: This study aimed to describe breast pump-related problems and injuries and identify factors associated with these problems and injuries. Methods: Data were from the Infant Feeding Practices Study II; mothers were recruited from a nationally distributed consumer opinion panel. Mothers were asked about breast pump use, problems, and injuries at infant ages 2, 5, and 7 months. Survival analysis was used to identify factors associated with pump-related problems and injuries. Results: The sample included 1844 mothers. About 62% and 15% of mothers reported pump-related problems and injuries, respectively. The most commonly reported problem was that the pump did not extract enough milk and the most commonly reported injury was sore nipples. Using a battery-operated pump and intending to breastfeed less than 12 months were associated with higher risks of pump-related problems and injury. Learning from a friend to use the pump was associated with lower risk of pump-related problems, and using a manual pump and renting a pump were associated with a higher risk of problems. Conclusion: Our results suggest that problems and injuries associated with breast pump use can happen to mothers of all socioeconomic characteristics. Breastfeeding mothers may reduce their risks of problems and injury by not using battery-operated pumps and may reduce breast pump problems by not using manual pumps and by learning breast pump skills from a person rather than following written or video instructions.
Donor Human Milk Bank Data Collection in North America
Authors: Brownell Elizabeth A. , Lussier Mary M. , Herson Victor C. , Hagadorn James I. , Marinelli Kathleen A. 
Published: October 2013

Background: The Human Milk Banking Association of North America (HMBANA) is a nonprofit association that standardizes and facilitates the establishment and operation of donor human milk (DHM) banks in North America. Each HMBANA milk bank in the network collects data on the DHM it receives and distributes, but a centralized data repository does not yet exist. In 2010, the Food and Drug Administration recognized the need to collect and disseminate systematic, standardized DHM bank data and suggested that HMBANA develop a DHM data repository. Objectives: This study aimed to describe data currently collected by HMBANA DHM banks and evaluate feasibility and interest in participating in a centralized data repository. Methods: We conducted phone interviews with individuals in different HMBANA milk banks and summarized descriptive statistics. Results: Eight of 13 (61.5%) sites consented to participate. All respondents collected donor demographics, and half (50%; n = 4) rescreened donors after 6 months of continued donation. The definition of preterm milk varied between DHM banks (≤ 32 to ≤ 40 weeks). The specific computer program used to house the data also differed. Half (50%; n = 4) indicated that they would consider participation in a centralized repository. Conclusions: Without standardized data across all HMBANA sites, the creation of a centralized data repository is not yet feasible. Lack of standardization and transparency may deter implementation of donor milk programs in the neonatal intensive care unit setting and hinder benchmarking, research, and quality improvement initiatives.
DNA Methylation as a Biomarker for Preeclampsia
Authors: Anderson Cindy M. , Ralph Jody L. , Wright Michelle L. , Linggi Bryan , Ohm Joyce E. 
Published: October 2013

Background: Preeclampsia contributes significantly to pregnancy-associated morbidity and mortality as well as future risk of cardiovascular disease in mother and offspring, and preeclampsia in offspring. The lack of reliable methods for early detection limits the opportunities for prevention, diagnosis, and timely treatment. Purpose: The purpose of this study was to explore distinct DNA methylation patterns associated with preeclampsia in both maternal cells and fetal-derived tissue that represent potential biomarkers to predict future preeclampsia and inheritance in children. Method: A convenience sample of nulliparous women ( N = 55) in the first trimester of pregnancy was recruited for this prospective study. Genome-wide DNA methylation was quantified in first-trimester maternal peripheral white blood cells and placental chorionic tissue from normotensive women and those with preeclampsia ( n = 6/group). Results: Late-onset preeclampsia developed in 12.7% of women. Significant differences in DNA methylation were identified in 207 individual linked cytosine and guanine (CpG) sites in maternal white blood cells collected in the first trimester (132 sites with gain and 75 sites with loss of methylation), which were common to approximately 75% of the differentially methylated CpG sites identified in chorionic tissue of fetal origin. Conclusion: This study is the first to identify maternal epigenetic targets and common targets in fetal-derived tissue that represent putative biomarkers for early detection and heritable risk of preeclampsia. Findings may pave the way for diagnosis of preeclampsia prior to its clinical presentation and acute damaging effects, and the potential for prevention of the detrimental long-term sequelae.
Genetic diversity of natural orchardgrass (Dactylis glomerataL.) populations in three regions in Europe
Authors: Last Luisa , Widmer Franco , Fjellstad Wendy , Stoyanova Siyka , Kölliker Roland 
Published: October 2013

Abstract

Background

Dactylis glomerata (orchardgrass or cocksfoot) is a forage crop of agronomic importance comprising high phenotypic plasticity and variability. Although the genus Dactylis has been studied quite well within the past century, little is known about the genetic diversity and population patterns of natural populations from geographically distinct grassland regions in Europe. The objectives of this study were to test the ploidy level of 59 natural and semi-natural populations of D. glomerata, to investigate genetic diversity, differentiation patterns within and among the three geographic regions, and to evaluate selected populations for their value as genetic resources.

Results
Among 1861 plants from 20 Swiss, 20 Bulgarian and 19 Norwegian populations of D. glomerata, exclusively tetraploid individuals were identified based on 29 SSR markers. The average expected heterozygosity (HE,C) ranged from 0.44 to 0.59 and was highest in the Norwegian region. The total number of rare alleles was high, accounting for 59.9% of the amplified alleles. 80.82% of the investigated individuals could be assigned to their respective geographic region based on allele frequencies. Average genetic distances were low despite large geographic distances and ranged from D = 0.09 to 0.29 among populations.

Conclusions
All three case study regions revealed high genetic variability of tetraploid D. glomerata within selected populations and numerous rare and localized alleles which were geographically unique. The large, permanent grassland patches in Bulgaria provided a high genetic diversity, while fragmented, semi-natural grassland in the Norwegian region provided a high amount of rare, localized alleles, which have to be considered in conservation and breeding strategies. Therefore, the selected grassland populations investigated conserve a large pool of genetic resources and provide valuable sources for forage crop breeding programs.
Do transgenesis and marker-assisted backcross breeding produce substantially equivalent plants? - A comparative study of transgenic and backcross rice carrying bacterial blight resistant gene Xa21
Authors: Gao Lifen , Cao Yinghao , Xia Zhihui , Jiang Guanghuai , Liu Guozhen , Zhang Weixiong , Zhai Wenxue 
Published: October 2013

Abstract

Background
The potential impact of genetically modified (GM) plants on human health has attracted much attention worldwide, and the issue remains controversial. This is in sharp contrast to the broad acceptance of plants produced by breeding through Marker Assisted Backcrossing (MAB).

Results
Focusing on transcriptome variation and perturbation to signaling pathways, we assessed the molecular and biological aspects of substantial equivalence, a general principle for food safety endorsed by the Food and Agricultural Organization and the World Health Organization, between a transgenic crop and a plant from MAB breeding. We compared a transgenic rice line (DXT) and a MAB rice line (DXB), both of which contain the gene Xa21 providing resistance to bacterial leaf blight. By using Next-Generation sequencing data of DXT, DXB and their parental line (D62B), we compared the transcriptome variation of DXT and DXB. Remarkably, DXT had 43% fewer differentially expressed genes (DEGs) than DXB. The genes exclusively expressed in DXT and in DXB have pathogen and stress defense functions. Functional categories of DEGs in DXT were comparable to that in DXB, and seven of the eleven pathways significantly affected by transgenesis were also perturbed by MAB breeding.

Conclusions
These results indicated that the transgenic rice and rice from MAB breeding are substantial equivalent at the transcriptome level, and paved a way for further study of transgenic rice, e.g., understanding the chemical and nutritional properties of the DEGs identified in the current study.
Enterohepatic bacterial infections dysregulate the FGF15-FGFR4 endocrine axis
Authors: Romain Guillaume , Tremblay Sarah , Arena Ellen T , Antunes L Caetano M , Covey Scott , Chow Michael T , Finlay B Brett , Menendez Alfredo 
Published: October 2013

AbstractBackgroundEnterohepatic bacterial infections have the potential to affect multiple physiological processes of the body. Fibroblast growth factor 15/19 (FGF15 in mice, FGF19 in humans) is a hormone that functions as a central regulator of glucose, lipid and bile acid metabolism. FGF15/19 is produced in the intestine and exert its actions on the liver by signaling through the FGFR4-βKlotho receptor complex. Here, we examined thein vivoeffects of enterohepatic bacterial infection over the FGF15 endocrine axis.ResultsInfection triggered significant reductions in the intestinal expression ofFgf15and its hepatic receptor components (Fgfr4andKlb(βKlotho)). Infection also resulted in alterations of the expression pattern of genes involved in hepatobiliary function, marked reduction in gallbladder bile volumes and accumulation of hepatic cholesterol and triglycerides. The decrease in ilealFgf15expression was associated with liver bacterial colonization and hepatobiliary pathophysiology rather than with direct intestinal bacterial pathogenesis.ConclusionsBacterial pathogens of the enterohepatic system can disturb the homeostasis of the FGF15/19-FGFR4 endocrine axis. These results open up a possible link between FGF15/19-FGFR4 disruptions and the metabolic and nutritional disorders observed in infectious diseases.
Segmental arterial mediolysis of left gastric artery: a case report and review of pathology
Authors: Tabassum Azra , Sasani Sanaz , Majid Adeeb J , Henderson Christopher , Merrett Neil D 
Published: October 2013

Abstract

Background
Segmental arterial mediolysis (SAM) is a rare non inflammatory vascular disease that can present with massive haemorrhage, which may lead to death without prompt surgical intervention.

Case presentation
A 60 years old Aboriginal female presented with life threatening, spontaneous intra-abdominal bleeding requiring an emergency laparotomy. The source of bleeding was found to be ruptured left gastric artery. A total gastrectomy was performed as a damage control procedure. A staged Roux-en-Y oesophago-jejunostomy with Hunt Lawrence pouch reconstruction was undertaken thirty six hours later. Histopathological findings revealed evidence of non-inflammatory segmental vascular damage with microaneurysm, consistent with segmental arterial mediolysis.

Conclusion
Prompt resuscitation and surgical intervention can decrease the morbidity and mortality in this rare clinical entity.
Death after percutaneous dilatational tracheostomy: a systematic review and analysis of risk factors
Authors: Simon Marcel , Metschke Maria , Braune Stephan A , Püschel Klaus , Kluge Stefan 
Published: October 2013

Abstract
Introduction
Since the introduction and widespread acceptance of percutaneous techniques in the intensive care unit (ICU) setting, the number of critically ill patients undergoing tracheostomy has steadily increased. However, this procedure can be associated with major complications, including death. The purpose of this study is to estimate the incidence and analyze the causes of lethal complications due to percutaneous dilatational tracheostomy (PDT).

Methods
We analyzed cases of lethal outcome due to complications from PDT including cases published between 1985 and April 2013. A systematic literature search was performed and unpublished cases from our own departmental records were retrospectively analyzed.

Results
A total of 71 cases of lethal outcome following PDT were identified including 68 published cases and 3 of our own patients. The incidence of lethal complications was calculated to be 0.17%. Of the fatal complications, 31.0% occurred during the procedure and 49.3% within seven days of the procedure. The main causes of death were: hemorrhage (38.0%), airway complications (29.6%), tracheal perforation (15.5%), and pneumothorax (5.6%). We found specific risk factors for complications in 73.2% of patients, 25.4% of patients had more than one risk factor. Bronchoscopic guidance was used in only 46.5% of cases.

Conclusions
According to this analysis, PDT-related death occurs in 1 out of 600 patients receiving a PDT. Careful patient selection, bronchoscopic guidance, and securing the tracheal cannula with sutures are likely to reduce complication rates.
Plasticity of lung development in the amphibian, Xenopus laevis
Authors: Rose Christopher S. , James Brandon 
Published: October 2013

Summary
Contrary to previous studies, we found that Xenopus laevis tadpoles raised in normoxic water without access to air can routinely complete metamorphosis with lungs that are either severely stunted and uninflated or absent altogether. This is the first demonstration that lung development in a tetrapod can be inhibited by environmental factors and that a tetrapod that relies significantly on lung respiration under unstressed conditions can be raised to forego this function without adverse effects. This study compared lung development in untreated, air-deprived (AD) and air-restored (AR) tadpoles and frogs using whole mounts, histology, BrdU labeling of cell division and antibody staining of smooth muscle actin. We also examined the relationship of swimming and breathing behaviors to lung recovery in AR animals. Inhibition and recovery of lung development occurred at the stage of lung inflation. Lung recovery in AR tadpoles occurred at a predictable and rapid rate and correlated with changes in swimming and breathing behavior. It thus presents a new experimental model for investigating the role of mechanical forces in lung development. Lung recovery in AR frogs was unpredictable and did not correlate with behavioral changes. Its low frequency of occurrence could be attributed to developmental, physical and behavioral changes, the effects of which increase with size and age. Plasticity of lung inflation at tadpole stages and loss of plasticity at postmetamorphic stages offer new insights into the role of developmental plasticity in amphibian lung loss and life history evolution.
The effects of the monetary policy stance on the transmission mechanism
Authors: Galvao Ana Beatriz , Marcellino Massimiliano 
Published: October 2013

AbstractThis paper contributes to the literature on changes in the transmission mechanism of monetary policy by introducing a model whose parameter evolution explicitly depends on the stance of monetary policy. The model, a structural break endogenous threshold VAR, also captures changes in the variance of shocks, and allows for a break in the parameters at an estimated time. We show that the transmission is asymmetric depending on the extention of the deviation of the actual policy rate from the one required by the Taylor rule. When the policy stance is tight – actual rate is higher than the one implied by the Taylor rule – contractionary shocks have stronger negative effects on output and prices.
Antihypertensive Treatment and Resistant Hypertension in Patients With Type 1 Diabetes by Stages of Diabetic Nephropathy
Authors: Lithovius Raija , Harjutsalo Valma , Forsblom Carol , Saraheimo Markku , Groop Per-Henrik ,   on behalf of the FinnDiane Study Group
Published: October 2013

OBJECTIVE
To assess blood pressure (BP) control, antihypertensive treatment, and prevalence of resistant hypertension (RH) in patients with type 1 diabetes stratified by stage of diabetic nephropathy.

RESEARCH DESIGN AND METHODS
This cross-sectional study included a nationally representative cohort of patients with type 1 diabetes (N = 3,678) from the Finnish Diabetic Nephropathy Study (FinnDiane). The data were linked to the Drug Prescription Register to obtain purchases of antihypertensive drugs 6 months prior to the baseline visit. The treatment targets were based on the American Diabetes Association guidelines. RH was defined as failure to reach BP target despite the use of three or more antihypertensive drugs of different classes (one of which was a diuretic).

RESULTS
In patients with normal albumin excretion rate, 14.1% were on antihypertensive treatment and 74.6% of them had uncontrolled BP despite treatment. The corresponding figures were 60.5 and 71.2% for the microalbuminuric patients, 90.3 and 80.0% for the macroalbuminuric patients, 88.6 and 88.1% for dialysis, and 91.2 and 90.4% for kidney-transplanted patients. The prevalence of RH was 1.2% in the normoalbuminuric, 4.7% in the microalbuminuric, 28.1% in the macroalbuminuric, 36.6% in the dialysis, and 26.3% in the kidney transplant groups. Age (odds ratio 1.04 [95% CI 1.02–1.05]), estimated glomerular filtration rate (0.97 [0.96–0.97]), waist-to-hip ratio (1.44 [1.15–1.80]), triglycerides (1.19 [1.01–1.40]), microalbuminuria (2.58 [1.43–4.67]), and macroalbuminuria (5.61 [3.20–9.84]) were independently associated with RH.

CONCLUSIONS
The prevalence of uncontrolled hypertension and RH increases with advanced diabetic nephropathy. These data suggest that there is an urgent need for improvement of antihypertensive treatment.
Association between subcutaneous fat thickness measured on thoracic radiographs and body condition score in dogs
Authors: Linder Deborah E. , Freeman Lisa M. , Sutherland-Smith James 
Published: October 2013

Abstract
Objective—To determine whether subcutaneous fat thickness measured on thoracic radiographs was associated with body condition score (BCS) in dogs.
Animals—87 client-owned dogs (41 males and 46 females) with a median age of 10.0 years (range, 1 to 16 years) and median weight of 20.3 kg (range, 3.1 to 58.0 kg).
Procedures—Age, sex, body weight, and breed were recorded. Body condition scores (scale from 1 to 9) and muscle condition scores were assigned by a single investigator. Subcutaneous fat thickness was measured at the level of the eighth rib head on a dorsoventral or ventrodorsal radiographic view of the thorax by a single investigator. Ratios of subcutaneous fat thickness to the width of the midbody of T8 on the ventrodorsal or dorsoventral radiographic view (T8 ratio) and to the length of the midbody of T4 on a right lateral radiographic view (T4 ratio) were calculated and compared with BCS by means of the Spearman correlation method.
Results—Median BCS was 6 (range, 1 to 9), and all muscle condition scores were represented. There were significant correlations between BCS and T4 ratio (r = 0.86) and between BCS and T8 ratio (r = 0.84).
Conclusions and Clinical Relevance—Results indicated that in this population, there was a significant association between BCS and subcutaneous fat thickness measured on thoracic radiographs. Findings suggested that measuring subcutaneous fat thickness could aid in the retrospective assignment of BCS in studies involving dogs in which BCS was not recorded in the medical record.
Adapting the CEFR for the Classroom Assessment of Young Learners’ Writing
Authors: Hasselgreen Angela 
Published: October 2013

Abstract: This article concerns the contribution that feedback makes to valid classroom assessment of the writing of young learners (YLs), defined here as approximately 9–13 years old. It shows that a scale of descriptors adapted from the Common European Framework of Reference for Languages (CEFR; Council of Europe, 2001) can play a central role in this assessment. The article presents a research project, AYLLIT (Assessment of Young Learner Literacy), which developed a CEFR-based instrument and guidelines for teachers, to provide their pupils with feedback that may allow them to see progress and lead to further learning. It sets out the procedures followed in the project and examines the extent to which its outcomes enable teachers to give feedback that contributes to valid classroom assessment.