bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of miR‐122 promotes the hepatic differentiation and maturation of mouse ESCs through a miR‐122/FoxA1/HNF4a‐positive feedback loop
Authors: Deng Xiao‐Geng , Qiu Rong‐Lin , Wu Yao‐Hao , Li Zhi‐Xi , Xie Ping , Zhang Jie , Zhou Jia‐Jia , Zeng Le‐Xiang , Tang Jing , Maharjan Anu , Deng Jie‐Min 
Published: July 2013

AbstractBackground & AimsmicroRNA‐122 is the only identified liver‐specific miRNA and plays a crucial role in liver development, maintenance of hepatic homeostasis as well as tumourigenesis. In our previous differentiation of ESCs into hepatocytes, microRNA‐122 (miR‐122) was expressed at a relatively low level. Here, we aim to elucidate the effect and underlying mechanisms of miR‐122 during differentiation of ESCs into hepatocytes.MethodsMouse ESCs were initially induced towards HPCs by activin A, FGF‐4 and sodium butyrate and were subsequently transfected with a recombinant adenovirus expressing vector pAV.Ex1d‐CMV>miR‐122/IRES/eGFP 9 days after induction. Cells were analysed by real‐time PCR, immunofluorescence, flow cytometry, microscopy and functional assays. Furthermore, microarray analysis was performed.ResultsWe demonstrated that overexpression of miR‐122 could effectively promote hepatic differentiation and maturation, as assessed by morphological and functional tests. The microarray analysis revealed that 323 genes were down‐regulated, whereas 59 were up‐regulated. Particularly, two liver‐specific transcription factors, FoxA1 and HNF4a, were significantly up‐regulated. Moreover, the expression of E‐cadherin was dramatically increased and the proliferation of HPCs was suppressed, whereas knockdown of FoxA1 reduced E‐cadherin expression and increased the proliferation of HPCs. In addition, the expression levels of FoxA1, HNF4a and E‐cadherin in time‐course transfection experiments with miR‐122 were not significantly increased except in cells in which transfection with miR‐122 occurred 9 days after induction.ConclusionOverexpression of miR‐122 at an appropriate stage could promote hepatic differentiation and maturation by regulating the balance between proliferation and differentiation, as well as the balance between EMT and MET, partially through a miR‐122/FoxA1/HNF4a‐positive feedback loop.
Psychiatric symptoms may contribute to poor quality of life in adolescents with migraine
Authors: Paschoal Joelma Karin Sagica Fernandes , Lin Jaime , Pinho Ricardo Silva , Andreoni Solange , Minett Thais Soares Cianciarullo , Vitalle Maria Sylvia de Souza , Fisberg Mauro , Peres Mario Fernando Prieto , Vilanova Luiz Celso Pereira , Masruha Marcelo Rodrigues 
Published: July 2013

AbstractBackgroundThe impact of migraine on quality of life (QOL) can be aggravated by other comorbid factors. The aim of the present study was to assess the differences in the QOL of adolescents with chronic migraine, episodic migraine, and healthy adolescents, and whether the differences in QOL among the diagnostic groups were associated with the presence of self‐reported psychiatric symptoms, such as depression and anxiety.MethodsA total of 157 adolescents (aged 15–19 years old) were included in the study. Fifty patients had episodic migraine, 56 patients suffered from chronic migraine, and 51 healthy adolescents were controls. All of the participants responded to a detailed headache questionnaire, the Medical Outcomes Trust 36‐Item Short‐form Health Survey, the State–Trait Anxiety Inventory and the Beck Depression Inventory.ResultsChronic migraine patients showed a significantly lower QOL than the control subjects in five dimensions of the Medical Outcomes Trust 36‐Item Short‐form Health Survey, and lower QOL than the episodic migraine patients in four dimensions. High levels of self‐reported depressive symptoms were associated with lower QOL in five dimensions and high levels of self‐reported anxiety were associated with lower QOL in four dimensions.ConclusionsThe QOL of adolescent migraine sufferers may be aggravated not only by migraine but also by other factors, such as anxiety and depressive symptoms, which may contribute to the poor QOL in adolescents suffering from migraine.
High‐Performance Photoinitiating Systems for Free Radical Photopolymerization. Application to Holographic Recording
Authors: Ibrahim Ahmad , Stefano Luciano Di , Tarzi Olga , Tar Haja , Ley Christian , Allonas Xavier 
Published: July 2013

AbstractThe photoinitiating systems (PIS) usable in free radical photopolymerization are briefly reviewed. Apart conventional Type I and Type II photoinitiators, three‐component PIS exhibiting a photocyclic behavior are discussed. The mechanisms of photoreaction are presented, highlighting the role of a redox additive which reacts with the photoinitiator photoproduct. Recent applications in holography recording are described showing that the selection of a PIS plays a crucial role in this application.
Genetic diversity of the root‐knot nematode Meloidogyne ethiopica and development of a species‐specific SCAR marker for its diagnosis
Authors: Correa V. R. , Mattos V. S. , Almeida M. R. A. , Santos M. F. A. , Tigano M. S. , Castagnone‐Sereno P. , Carneiro R. M. D. G. 
Published: July 2013

Meloidogyne ethiopica is an important nematode pathogen causing serious economic damage to grapevine in Chile. In Brazil, M. ethiopica has been detected with low frequency in kiwifruit and other crops. The objectives of this study were to evaluate the intraspecific genetic variability of M. ethiopica isolates from Brazil and Chile using AFLP and RAPD markers and to develop a species‐specific SCAR‐PCR assay for its diagnosis. Fourteen isolates were obtained from different geographic regions or host plants. Three isolates of an undescribed Meloidogyne species and one isolate of M. ethiopica from Kenya were included in the analysis. The results showed a low level of diversity among the M. ethiopica isolates, regardless of their geographical distribution or host plant origin. The three isolates of Meloidogyne sp. showed a high homogeneity and clustered separately from M. ethiopica (100% bootstrap). RAPD screenings of M. ethiopica allowed the identification of a differential DNA fragment that was converted into a SCAR marker. Using genomic DNA from pooled nematodes as a template, PCR amplification with primers designed from this species‐specific SCAR produced a fragment of 350 bp in all 14 isolates of M. ethiopica tested, in contrast with other species tested. This primer pair also allowed successful amplification of DNA from single nematodes, either juveniles or females and when used in multiplex PCR reactions containing mixtures of other root‐knot nematode species, thus showing the sensitivity of the assay. Therefore, the method developed here has potential for application in routine diagnostic procedures.
An assay for quantitative virulence in Rhynchosporium commune reveals an association between effector genotype and virulence
Authors: Stefansson T. S. , Willi Y. , Croll D. , McDonald B. A. 
Published: July 2013

Detailed knowledge of the evolutionary genetics of virulence is needed to understand and predict host–pathogen dynamics. This study used a virulence assay based on digital image analysis and treated virulence as a quantitative rather than a binary trait. Such quantitative data may better reflect the genetic underpinning of virulence in many pathogen systems and provide better resolution in statistical investigations. A greenhouse experiment based on a common garden design was conducted to measure virulence (% of leaf area covered by lesions) of 126 genetically distinct isolates of the barley scald pathogen, Rhynchosporium commune, originating from nine field populations around the world. Virulence in this pathosystem was found to be a quantitative trait with a continuous distribution in all populations. By comparing population genetic differentiation for virulence and neutral microsatellite markers (i.e. a QST/GST comparison), evidence that virulence is under stabilizing selection across populations was found. Heritability values were high and ranged from 0·52 to 0·96 with a mean heritability of 0·84. Virulence was positively correlated with spore production as predicted by the trade‐off theory of virulence evolution. Furthermore, an association analysis between virulence and sequence haplotypes of three known necrosis‐inducing effector genes (NIP1, NIP2 and NIP3) revealed a significant effect of NIP2 haplotypes and NIP1 deletions. Overall, the results support a quantitative model for virulence in the R. commune–barley pathosystem and very high evolutionary potential for this trait.
A systematic review of factors associated with the deferral of donors failing to meet low haemoglobin thresholds
Authors: Smith Graham A. , Fisher Sheila A. , Dorée Carolyn , Roberts David J. 
Published: July 2013

SUMMARYBackground/ObjectivesBlood donors attending a donation session may be deemed ineligible to donate blood due to a failure to meet low haemoglobin (Hb) thresholds. Several studies have identified factors associated with a donor falling below these Hb thresholds. A review of these factors will inform future prospective studies and form the basis for predictive models of deferral due to low Hb.Materials/MethodsStudies were identified by searching MEDLINE, EMBASE, The Cochrane Library and the WHO International Clinical Trials Registry from 1980 to September 2012. Demographic data, donor history, haematological/biological factors and the primary outcome of deferral due to low Hb were extracted. Analyses were descriptive and quantitative; pooled odds ratios (ORs) were obtained by meta‐analysis.ResultsFifty‐five studies met the inclusion criteria. A consistently higher rate of low Hb deferral was reported in females compared with males; meta‐analysis showed a significantly greater risk of deferral due to low Hb in females compared with males in studies with universal Hb thresholds for males and females (OR 14·91, 95% confidence interval (CI) 12·82–17·34) and in studies with sex‐specific Hb thresholds (OR 8·19, 95% CI 4·88–13·74). Greater rates of deferral due to low Hb were also associated with increasing age, higher ambient temperature, low body weight, shorter inter‐donation interval and in donors of Hispanic or African descent.ConclusionThis work will help to define the criteria that should be considered in any large scale study of blood donor deferral, especially those that measure or aim to change failure to meet low Hb thresholds.
A mutation in the Arabidopsis thaliana cell wall biosynthesis gene pectin methylesterase 3 as well as its aberrant expression cause hypersensitivity specifically to Zn
Authors: Weber Michael , Deinlein Ulrich , Fischer Sina , Rogowski Michaela , Geimer Stefan , Tenhaken Raimund , Clemens Stephan 
Published: July 2013

SummaryDefects in metal homeostasis factors are often accompanied by the loss of metal tolerance. Therefore, we screened for mutants with compromised growth in the presence of excess Zn2+ in order to identify factors involved in Zn biology in plants. Here we report the isolation of six ozs (overly Zn sensitive) ethyl methanesulfonate Arabidopsis thaliana mutants with contrasting patterns of metal sensitivity, and the molecular characterization of two mutants hypersensitive specifically to Zn2+. Mutant ozs1 represents a non‐functional allele of the vacuolar Zn transporter AtMTP1, providing additional genetic evidence for its major role in Zn2+ tolerance in seedlings. Mutant ozs2 carries a semi‐dominant mutation in the gene encoding pectin methylesterase 3 (AtPME3), an enzyme catalyzing demethylesterification of pectin. The mutation results in impaired proteolytic processing of AtPME3. Ectopic expression of AtPME3 causes strong Zn2+ hypersensitivity that is tightly correlated with transcript abundance. Together these observations suggest detrimental effects on Golgi‐localized processes. The ozs2 but not the ozs1 phenotype can be suppressed by extra Ca2+, indicating changes in apoplastic cation‐binding capacity. However, we did not detect any changes in bulk metal‐binding capacity, overall pectin methylesterification status or cell wall ultrastructure in ozs2, leading us to hypothesize that the ozs2 mutation causes hypersensitivity towards the specific interference of Zn ions with cell wall‐controlled growth processes.
Pathogen reduction treatment alters the immunomodulatory capacity of buffy coat–derived platelet concentrates
Authors: Loh Yen S. , Johnson Lacey , Kwok Matthew , Marks Denese C. 
Published: July 2013

BackgroundStorage of platelet concentrates (PCs) after Mirasol pathogen reduction technology (PRT) treatment changes platelet (PLT) surface marker expression and secretion of immunomodulatory factors. Given that PLTs are known to participate in immune function, PRT may alter the way PLTs interact with the immune cells of a recipient upon transfusion. As such, the aim of this study was to assess the effects of PRT treatment on the functional ability of PLTs to interact with peripheral blood mononuclear cells (PBMNCs).Study Design and MethodsBuffy coat–derived PCs were pooled and split to obtain matched pairs. One unit was treated using the Mirasol PRT system, while the control PC remained untreated. After 5 days of storage, either the PLTs or the PLT supernatants from the PCs were cocultured with PBMNCs, with or without lipopolysaccharide (LPS). The immunomodulatory factors secreted into culture medium after coculture were examined.ResultsPRT‐treated PLTs and PLT supernatant significantly increased the interleukin (IL)‐8 concentration, which was manifested only in the presence of LPS. Conversely, PRT‐treated PLTs secreted less soluble P‐selectin (sCD62P) upon coculture with PBMNCs.ConclusionPRT‐treatment induced differential secretion of IL‐8 and sCD62P during coculture, which may be attributed to either bioactive substances present in PLT supernatant or as a result of cell–cell interactions.
Successful management of severe hemolytic disease of the fetus due to anti‐Jsb using intrauterine transfusions with serial maternal blood donations: a case report and a review of the literature
Authors: Al Riyami Arwa Z. , Al Salmani Moza , Al Hashami Sabria , Al Mahrooqi Sabah , Al Hinai Sumaiya , Al Balushi Halima , Al Riyami Nihal , Gowri V. , Al Dughaishi Tamima , Al Hosni Saif , Al‐Khabori Murtadha , Al‐Farsi Khalil , Al Huneini Mohammed , Alkindi Salam 
Published: July 2013

BackgroundThe management of pregnant women with anti‐Jsb is challenging due to the paucity of antigen‐negative blood for fetal and neonatal transfusion.Case ReportA 29‐year‐old woman with anti‐Jsb was referred for assessment of recurrent fetal losses. With the presence of the sister as a historically matched donor, she was planned for active surveillance for fetal anemia during pregnancy.Study Design and MethodsThe fetus remained well until 21 weeks of gestation when signs of fetal anemia and early hydrops fetalis were noted. Anti‐Jsb titer was at 128. The sister's red blood cells (RBCs) were cross‐match incompatible. Urgent intrauterine transfusion (IUT) was performed with washed irradiated maternal RBCs, donated after cessation of heparin. The mother was given intravenous iron (IV‐Fe) and continued on weekly recombinant human erythropoietin (rHu‐EPO).ResultsRepeated IUTs were needed every 1 to 3 weeks. Throughout a 7‐week period, three maternal donations were performed with total donated whole blood volume of 1250 mL, supporting four IUTs. At 29 weeks of gestation, the procedure was complicated by umbilical cord hematoma necessitating urgent cesarean section. A male newborn was delivered, transfused at birth, and subsequently treated with phototherapy and five top‐up transfusions.ConclusionThis case represents a successful example of managing hemolytic disease of the fetus due to a rare antibody using maternal blood. It also supports previous data on safety of maternal donations during pregnancy and the use of combination of rHu‐EPO and IV‐Fe as a supportive measure.
A pilot study of the validation of percutaneous testing in cats
Authors: Rossi Michael A. , Messinger Linda , Olivry Thierry , Hoontrakoon Raweewan 
Published: July 2013

BackgroundIntradermal testing is useful for the identification of environmental allergens to which cats could be hypersensitive; intradermal test reactions are often subtle and difficult to interpret in cats. Percutaneous testing is the standard technique for the detection of significant environmental allergens in people, but it has not yet been evaluated in cats with hypersensitivity dermatitis.Hypothesis/ObjectivesThe purpose of this study was to evaluate and compare the skin test responses of healthy cats to percutaneous application and intradermal injections of control solutions.MethodsTen clinically healthy cats were studied. Percutaneous applications of 0.0275 and 0.1 mg/mL aqueous histamine, 6 mg/mL glycerinated histamine, 0.9% buffered saline and 50% glycerosaline solution were performed using Greer Pick (Greer Laboratories, Lenoir, NC, USA) and Duotip‐Test II (Lincoln Diagnostics, Decatur, IL, USA) percutaneous applicators. Reactions were compared with intradermal injections of 0.0275 mg/mL aqueous histamine and 0.9% buffered saline as controls.ResultsPositive responses to histamine solutions were significantly greater with the Greer Pick than with the Duotip‐Test II. There were no significant differences between the histamine reactions using the Greer Pick applicator and the intradermal injections. Percutaneous reactions to histamine were more well demarcated and easier to read than intradermal injection reactions. Reactions to the saline controls were not noted.Conclusions and clinical importancePercutaneous application of 6 mg/mL glycerinated histamine solution, 50% glycerosaline solution and 0.9% buffered saline produced similar positive and negative control wheals. These observations warrant further studies of percutaneous allergen testing in cats with hypersensitivity dermatitis.
“Then Their Eyes Were Opened”: Pentecostal Reflections on the Church’s Scripture and the Lord’s Supper
Authors: Green Chris E.W. 
Published: July 2013

Abstract
This paper explores the relationship of Scripture to the Lord’s Supper in and for Pentecostal theology and praxis, drawing on a figurative reading of the story of the Emmaus disciples (Luke 24) as a paradigm. The paper’s central claim is that faithful eucharistic participation is indispensable to the faithful reading of Scripture — and vice versa. It is just as our lives are consumed by the Eucharist event that our eyes are opened to see the Jesus of whom the Scriptures testify and that we are made apt for the transforming work of the Spirit. On this basis, a call is made for a robust and authentically Pentecostal sacramentality to orient and ground uses of Scripture in and by the Pentecostal ecclesial community. In addition to fellow Pentecostals, dialogue partners from the wider Christian tradition are also engaged, including, most prominently, Rowan Williams, Jean-Luc Marion, and Robert W. Jenson.
Pentecostal Epiclesis: A Model for Teaching and Learning
Authors: Alvarado Johnathan E. 
Published: July 2013

Abstract
The eucharistic practices of many mainline and historic Christian traditions have been co-opted into Pentecostalism’s theology and praxis. While this has been functional for the first one hundred years of Pentecostalism, a decidedly Pentecostal expression of epiclesis is still being developed and existing modes of eucharistic prayer are being understood. In this essay I examine epiclesis and note its natural intersection with Pentecostal theology as the inroad to an expanded theological dialogue. I define epiclesis historically and within a contemporary Pentecostal context. Finally, I argue that the mainline and historic churches could teach and learn from a cross-pollination of ideas with Pentecostal churches.
Ein Vorschlag zur Chronologie der 25. Dynastie in Ägypten
Authors: Bányai Michael 
Published: July 2013

Abstract
The first mention of Kush in the Assyrian sources comes in the context of the resolution of the conflict between Sargon and the rebellious prince of Ashdod, Iamani, in ca. 707 BCE. However, this event plays a critical role in our understanding of the chronology of the end of the 24th and beginning of the 25th Dynasties. This paper re-examines the relevant data pertaining to the period in an attempt to synchronize the historical and chronological relationships presented in the Assyrian and Egyptian sources. As a result of this work, a reversal of the conventional order of the kings Schabako-Schebtiko, which was obscured in Manetho’s epithoma and is thus exclusively a matter of modern reconstruction, is needed. The new chronology put forward by the author also necessitates the re-evaluation of several other documents essential to the history of this period.
The Urban Heat Island of the North-Central Texas Region and Its Relation to the 2011 Severe Texas Drought
Authors: Winguth A. M. E. , Kelp B. 
Published: July 2013

AbstractHourly surface temperature differences between Dallas–Fort Worth, Texas, metropolitan and rural sites have been used to calculate the urban heat island from 2001 to 2011. The heat island peaked after sunset and was particularly strong during the drought and heat wave in July 2011, reaching a single-day instantaneous maximum value of 5.4°C and a monthly mean maximum of 3.4°C, as compared with the 2001–11 July average of 2.4°C. This severe drought caused faster warming of rural locations relative to the metropolitan area in the morning as a result of lower soil moisture content, which led to an average negative heat island in July 2011 of −2.3°C at 1100 central standard time. The ground-based assessment of canopy air temperature at screening level has been supported by a remotely sensed surface estimate from the Moderate Resolution Imaging Spectroradiometer (MODIS) on board theTerrasatellite, highlighting a dual-peak maximum heat island in the major city centers of Dallas and Fort Worth. Both ground-based and remotely sensed spatial analyses of the maximum heat island indicate a northwest shift, the result of southeast winds in July 2011 of ~2 m s−1on average. There was an overall positive trend in the urban heat island of 0.14°C decade−1in the Dallas–Fort Worth metropolitan area from 2001 to 2011, due to rapid urbanization. Superimposed on this trend are significant interannual and decadal variations that influence the urban climate.
A Novel Scheme for Parameterizing Aerosol Processing in Warm Clouds
Authors: Lebo Zachary J. , Morrison Hugh 
Published: July 2013

Abstract
A novel two-moment bulk aerosol parameterization is derived from a state-of-the-art 2D bin microphysics model using power-law relationships and a semi-analytical technique for activation. The activation scheme predicts both number and mass of a lognormal aerosol distribution and permits the evolution of the modal mass with time. The newly developed bulk aerosol scheme is formulated for use in traditional two-moment bulk microphysics models. The new explicit scheme is compared with the 2D bin scheme and a simple scaling aerosol parameterization, in which all the aerosol processes are scaled to the respective cloud process rates, in a kinematic model with a specified flow field. Hybrid simulations in which the explicit activation formulation is coupled to the scaling parameterization are also performed. Model results demonstrate the significance of including a physically realistic representation of aerosols contained in haze, cloud droplets, and rain. It is shown that the explicit aerosol parameterization and scaling method predict similar bulk aerosol quantities and match the results of the 2D bin model only if an explicit treatment of aerosol activation—that is, both aerosol number and mass transfer because of activation—is included in the microphysics model.