bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lower plasma visceral protein concentrations are independently associated with higher mortality in patients on peritoneal dialysis
Authors: Huang Rong , Liu Yun , Wu Haishan , Guo Qunying , Yi Chunyan , Lin Jianxiong , Zhou Qian , Yu Xueqing , Yang Xiao 
Published: January 2015

Protein–energy wasting (PEW) is strongly associated with high mortality in continuous ambulatory peritoneal dialysis (CAPD) patients. However, its clinical assessment has not been well defined. The aim of the present study was to investigate the relationship between combined nutritional indicators and mortality in CAPD patients. In the present retrospective cohort study, a total of 885 incident CAPD patients were enrolled. Nutritional status at the initiation of CAPD was assessed by BMI and biochemical indices (serum albumin, prealbumin, transferrin, creatinine and total cholesterol). The primary outcome was all-cause mortality. Principal components factor analysis was used to identify the combined nutritional parameters. Their association with mortality was examined by multivariable-adjusted Cox models. The mean age was 47·4 (sd14·8) years, 59·2 % (n524) were male and 24·6 % (n218) were diabetic. Of the total patients, 130 (14·7 %) had BMI < 18·5 kg/m2, 439 (49·6 %) had albumin < 38 g/l ( < 3·8 g/dl), 303 (34·2 %) had prealbumin < 300 mg/l ( < 30 mg/dl), 404 (45·6 %) had transferrin < 2 g/l ( < 200 mg/dl), 501 (56·6 %) had total cholesterol < 5·2 mmol/l ( < 200 mg/dl) and 466 (52·7 %) had creatinine < 707 μmol/l ( < 8 mg/dl). Overall, three components such as visceral proteins, muscle-mass surrogate and BMI were extracted, which explained 69·95 % of the total variance of the nutritional parameters. After adjusting for demographic variables, co-morbid conditions, Hb, TAG and high-sensitivity C-reactive protein, the factor score of visceral proteins including albumin, prealbumin and transferrin was independently associated with mortality (hazard ratio 0·73, 95 % CI 0·60, 0·89;P= 0·002). Lower visceral protein concentrations may be independently associated with higher mortality in incident CAPD patients. Simultaneous measurements of serum albumin, prealbumin and transferrin could be helpful to monitor PEW.
Evaluation of dietary patterns among Norwegian postmenopausal women using plasma carotenoids as biomarkers
Authors: Markussen Marianne S. , Veierød Marit B. , Sakhi Amrit K. , Ellingjord-Dale Merete , Blomhoff Rune , Ursin Giske , Andersen Lene F. 
Published: January 2015

A number of studies have examined dietary patterns in various populations. However, to study to what extent such patterns capture meaningful differences in consumption of foods is of interest. In the present study, we identified important dietary patterns in Norwegian postmenopausal women (age 50–69 years,n361), and evaluated these patterns by examining their associations with plasma carotenoids. Diet was assessed by a 253-item FFQ. These 253 food items were categorised into forty-six food groups, and dietary patterns were identified using principal component analysis. We used the partial correlation coefficient (radj) and multiple linear regression analysis to examine the associations between the dietary patterns and the plasma carotenoids α-carotene, β-carotene, β-cryptoxanthin, lutein, lycopene and zeaxanthin. Overall, four dietary patterns were identified: the ‘Western’; ‘Vegetarian’; ‘Continental’; ‘High-protein’. The ‘Western’ dietary pattern scores were significantly inversely correlated with plasma lutein, zeaxanthin, lycopene and total carotenoids ( − 0·25 ≤ radj≤ − 0·13). The ‘Vegetarian’ dietary pattern scores were significantly positively correlated with all the plasma carotenoids (0·15 ≤ radj≤ 0·24). The ‘Continental’ dietary pattern scores were significantly inversely correlated with plasma lutein and α-carotene (radj= − 0·13). No significant association between the ‘High-protein’ dietary pattern scores and the plasma carotenoids was found. In conclusion, the healthy dietary pattern, the ‘Vegetarian’ pattern, is associated with a more favourable profile of the plasma carotenoids than our unhealthy dietary patterns, the ‘Western’ and ‘Continental’ patterns.
The effects of eating marine- or vegetable-fed farmed trout on the human plasma proteome profiles of healthy men
Authors: Rentsch Maria L. , Lametsch René , Bügel Susanne , Jessen Flemming , Lauritzen Lotte 
Published: January 2015

Most human intervention studies have examined the effects on a subset of risk factors, some of which may require long-term exposure. The plasma proteome may reflect the underlying changes in protein expression and activation, and this could be used to identify early risk markers. The aim of the present study was to evaluate the impact of regular fish intake on the plasma proteome. We recruited thirty healthy men aged 40 to 70 years, who were randomly allocated to a daily meal of chicken or trout raised on vegetable or marine feeds. Blood samples were collected before and after 8 weeks of intervention, and after the removal of the twelve most abundant proteins, plasma proteins were separated by two-dimensional gel electrophoresis. Protein spots < 66 kDa with a pI >4·3 visualised by silver staining were matched by two-dimensional imaging software. Within-subject changes in spots were compared between the treatment groups. Differentially affected spots were identified by matrix-assisted laser desorption ionisation-time of flight/time of flight MS and the human Swiss-Prot database. We found 23/681 abundant plasma protein spots, which were up- or down-regulated by the dietary treatment (P< 0·05, q< 0·30), and eighteen of these were identified. In each trout group, ten spots differed from those in subjects given the chicken meal, but only three of these were common, and only one spot differed between the two trout groups. In both groups, the affected plasma proteins were involved in biological processes such as regulation of vitamin A and haem transport, blood fibrinolysis and oxidative defence. Thus, regular fish intake affects the plasma proteome, and the changes may indicate novel mechanisms of effect.
Article 60 ICJ Statute, Interpretation Proceedings, and the Competing Concepts ofRes Judicata
Authors: KULICK ANDREAS 
Published: January 2015

AbstractThe judgment of the International Court of Justice (ICJ) of 11 November 2013, interpreting its 1962 judgment in the case concerning the Temple of Preah Vihear, inspires a reconsideration of the role of interpretation proceedings pursuant to Article 60 of the Statute of the Court. In particular, it invites us to take a closer look at the role and scope of the principle ofres judicatain public international law in general and as considered in the case law of the Court in particular. This contribution reveals the competing concepts of the principle in interpretation and consecutive original contentious proceedings, and promotes instead a uniform concept that avoids the conflict inherent in current approaches.Section 1 introduces Article 60 of the ICJ Statute (section 1.1.) and thereinafter views interpretation proceedings before the Court in light of the Court's case law, in particular its 2013 interpretation judgment in theTemplecase (section 1.2.). Section 2 constitutes the heart of the analysis and discusses what will be identified as the competing concepts ofres judicata. Section 2.1. presents the functions, concepts, and case law ofres judicatain public international law in general, whereas section 2.2. focuses specifically on the case law of the ICJ and the Permanent Court of International Justice (PCIJ) in this regard. Finally, section 2.3. concludes this study by setting out the approach that the author regards as appropriate to reconcile the competing concepts ofres judicata.
Burden and Standard of Proof in Defence Challenges to the Jurisdiction of the International Criminal Court
Authors: VAGIAS MICHAIL , FERENCZ JANOS 
Published: January 2015

AbstractThe jurisdiction of the International Criminal Court has remained largely uncontested during the first 10 years of its operation. Today, the jurisdictional cooling-off period seems to have run its course. The Prosecutor has opened the first Article 15 investigations and prosecutions in Kenya. The defence has been active in challenging the jurisdiction of the Court. Judges at the pre-trial stage have taken a more inquisitive approach to jurisdictional assessments. This awakening has led to the identification of novel legal issues. One of them is the applicable burden and standard of proof for defence challenges to jurisdiction. So far, this issue has been addressed largely through interpretation of the Statute. The Court's first decisions seem to fluctuate significantly on this point. From pronouncements accepting that such burden and standard do exist and seeking to articulate them, to rulings implying that they do not exist altogether, the Court's case law reveals a measure of inconsistency and a lack of reasoning. This article seeks to expose the different positions assumed on the matter, typically as a result of the judges’ efforts to balance procedural efficiency and fair trial considerations. In doing so, we will reflect critically on the causes and effects of the current state of the law and propose a reorientation of the case law through the use of other relevant international jurisprudence.
The Evidence of Glioblastoma Heterogeneity
Authors: Soeda Akio , Hara Akira , Kunisada Takahiro , Yoshimura Shin-ichi , Iwama Toru , Park Deric M. 
Published: January 2015

AbstractCancers are composed of heterogeneous combinations of cells that exhibit distinct phenotypic characteristics and proliferative potentials. Because most cancers have a clonal origin, cancer stem cells (CSCs) must generate phenotypically diverse progenies including mature CSCs that can self-renew indefinitely and differentiated cancer cells that possess limited proliferative potential. However, no convincing evidence exists to suggest that only single CSCs are representative of patients' tumors. To investigate the CSCs' diversity, we established 4 subclones from a glioblastoma patient. These subclones were subsequently propagated and analyzed. The morphology, the self-renewal and proliferative capacities of the subclones differed. Fluorescence-activated cell sorting and cDNA-microarray analyses revealed that each subclone was composed of distinct populations of cells. Moreover, the sensitivities of the subclones to an inhibitor of epidermal growth factor receptor were dissimilar. In a mouse model featuring xenografts of the subclones, the progression and invasion of tumors and animal survival were also different. Here, we present clear evidence that a brain tumor contains heterogeneous subclones that exhibit dissimilar morphologies and self-renewal and proliferative capacities. Our results suggest that single cell-derived subclones from a patient can produce phenotypically heterogeneous self-renewing progenies in both in vitro and in vivo settings.
Wnt5a promotes cancer cell invasion and proliferation by receptor-mediated endocytosis-dependent and -independent mechanisms, respectively
Authors: Shojima Kensaku , Sato Akira , Hanaki Hideaki , Tsujimoto Ikuko , Nakamura Masahiro , Hattori Kazunari , Sato Yuji , Dohi Keiji , Hirata Michinari , Yamamoto Hideki , Kikuchi Akira 
Published: January 2015

AbstractWnt5a activates the Wnt/β-catenin-independent pathway and its overexpression is associated with tumor aggressiveness enhancing invasive activity. For this action, Wnt5a-induced receptor endocytosis with clathrin is required. Wnt5a expression was previously believed to be associated with cancer cell motility but not proliferation. Recently, it was reported that Wnt5a is also implicated in cancer cell proliferation, but the mechanism was not clear. In this study, we generated a neutralizing anti-Wnt5a monoclonal antibody (mAb5A16) to investigate the mechanism by which Wnt5a regulates cancer cell proliferation. Wnt5a stimulated both invasion and proliferation of certain types of cancer cells, including HeLaS3 cervical cancer cells and A549 lung cancer cells although Wnt5a promoted invasion but not proliferation in other cancer cells such as KKLS gastric cancer cells. mAb5A16 did not affect the binding of Wnt5a to its receptor, but it suppressed Wnt5a-induced receptor-mediated endocytosis. mAb5A16 inhibited invasion but not proliferation of HeLaS3 and A549 cells. Wnt5a activated Src family kinases (SFKs) and Wnt5a-dependent cancer cell proliferation was dependent on SFKs, yet blockade of receptor-mediated endocytosis did not affect cancer cell proliferation and SFK activity. These results suggest that Wnt5a promotes invasion and proliferation of certain types of cancer cells through receptor-mediated endocytosis-dependent and -independent mechanisms, respectively.
Giant Electric Field Enhancement in Split Ring Resonators Featuring Nanometer-Sized Gaps
Authors: Bagiante S. , Enderli F. , Fabiańska J. , Sigg H. , Feurer T. 
Published: January 2015

AbstractToday's pulsed THz sources enable us to excite, probe and coherently control the vibrational or rotational dynamics of organic and inorganic materials on ultrafast time scales. Driven by standard laser sources THz electric field strengths of up to several MVm−1 have been reported and in order to reach even higher electric field strengths the use of dedicated electric field enhancement structures has been proposed. Here, we demonstrate resonant electric field enhancement structures, which concentrate the incident electric field in sub-diffraction size volumes and show an electric field enhancement as high as ~14,000 at 50 GHz. These values have been confirmed through a combination of near-field imaging experiments and electromagnetic simulations.
Transgenic mouse model expressing P53R172H, luciferase, EGFP and KRASG12D in a single open reading frame for live imaging of tumor
Authors: Ju Hye-Lim , Calvisi Diego F. , Moon Hyuk , Baek Sinhwa , Ribback Silvia , Dombrowski Frank , Cho Kyung Joo , Chung Sook In , Han Kwang-Hyub , Ro Simon Weonsang 
Published: January 2015

AbstractGenetically engineered mouse cancer models allow tumors to be imaged in vivo via co-expression of a reporter gene with a tumor-initiating gene. However, differential transcriptional and translational regulation between the tumor-initiating gene and the reporter gene can result in inconsistency between the actual tumor size and the size indicated by the imaging assay. To overcome this limitation, we developed a transgenic mouse in which two oncogenes, encoding P53R172H and KRASG12D, are expressed together with two reporter genes, encoding enhanced green fluorescent protein (EGFP) and firefly luciferase, in a single open reading frame following Cre-mediated DNA excision. Systemic administration of adenovirus encoding Cre to these mice induced specific transgene expression in the liver. Repeated bioluminescence imaging of the mice revealed a continuous increase in the bioluminescent signal over time. A strong correlation was found between the bioluminescent signal and actual tumor size. Interestingly, all liver tumors induced by P53R172H and KRASG12D in the model were hepatocellular adenomas. The mouse model was also used to trace cell proliferation in the epidermis via live fluorescence imaging. We anticipate that the transgenic mouse model will be useful for imaging tumor development in vivo and for investigating the oncogenic collaboration between P53R172H and KRASG12D.
In vitro reconstruction of branched tubular structures from lung epithelial cells in high cell concentration gradient environment
Authors: Hagiwara Masaya , Peng Fei , Ho Chih-Ming 
Published: January 2015

AbstractWe have succeeded in developing hollow branching structure in vitro commonly observed in lung airway using primary lung airway epithelial cells. Cell concentration gradient is the key factor that determines production of the branching cellular structures, as optimization of this component removes the need for heterotypic culture. The higher cell concentration leads to the more production of morphogens and increases the growth rate of cells. However, homogeneous high cell concentration does not make a branching structure. Branching requires sufficient space in which cells can grow from a high concentration toward a low concentration. Simulation performed using a reaction-diffusion model revealed that long-range inhibition prevents cells from branching when they are homogeneously spread in culture environments, while short-range activation from neighboring cells leads to positive feedback. Thus, a high cell concentration gradient is required to make branching structures. Spatial distributions of morphogens, such as BMP-4, play important roles in the pattern formation. This simple yet robust system provides an optimal platform for the further study and understanding of branching mechanisms in the lung airway and will facilitate chemical and genetic studies of lung morphogenesis programs.
Therapeutic mechanisms of high-frequency stimulation in Parkinson’s disease and neural restoration via loop-based reinforcement
Authors: Santaniello Sabato , McCarthy Michelle M. , Montgomery Erwin B. , Gale John T. , Kopell Nancy , Sarma Sridevi V. 
Published: January 2015

Significance
We investigated the therapeutic mechanisms of high-frequency stimulation (HFS) in Parkinson’s disease by developing a computational model of the cortico-basal ganglia-thalamo-cortical loop in normal and parkinsonian conditions under the effects of stimulation at several frequencies. We found that the stimulation injected in the loop elicits neural perturbations that travel along multiple pathways with different latencies and rendezvous in striatum (one of the basal ganglia). If the stimulation frequency is high enough, these perturbations overlap (reinforcement) and cause more regular, stimulus-locked firing patterns in striatum. Overlap is maximal at clinically relevant HFS and restores more normal activity in the remaining structures of the loop. This suggests that neural restoration and striatal reinforcement may be a therapeutic merit and mechanism of HFS, respectively.
Cranial vault trauma and selective mortality in medieval to early modern Denmark
Authors: Boldsen Jesper L. , Milner George R. , Weise Svenja 
Published: January 2015

Significance
Neurocranial fractures and their aftermath took a toll on people in premodern societies, much like today. Archaeological information on skeletal trauma, however, typically consists of mere tallies of injuries, much like other disease-related lesions. We quantify the increased risk of dying for men with healed cranial vault fractures, an approach that can be adapted to any pathological condition. In medieval to early modern Denmark, head-injured men experienced a relative risk of dying about double that of modern people, probably in large part because of differences in medical care and social support. This approach provides a means of measuring the extent, hence consequences, of excess injury and disease-related mortality across the full range of human societies extending into the distant past.
Src phosphorylation converts Mdm2 from a ubiquitinating to a neddylating E3 ligase
Authors: Batuello Christopher N. , Hauck Paula M. , Gendron Jaimie M. , Lehman Jason A. , Mayo Lindsey D. 
Published: January 2015

Significance
Conjugation of large polypeptides such as Neural precursor cell expressed, developmentally down-regulated 8 (Nedd8) and ubiquitin to proteins is a critical regulatory process for normal cell function. There are more than 1,000 E3 ligases that are involved in the ubiquitin pathway. The predominant activity of one E3, murine double minute-2 protein (Mdm2), has been characterized to mediate ubiquitination of the tumor suppressor p53 in response to genotoxic stress. We show that under growth conditions, active Src kinase binds to and phosphorylates Mdm2 at Y281 and Y302, which recruits the Nedd8 E2 enzyme, Ubc12. This recruitment converts Mdm2 to an E3 that neddylates p53. We provide the first evidence, to our knowledge, showing how phosphorylation may redirect ligase activity. This mechanism may be applicable to numerous E3 ligases and provides a basis for therapeutic intervention.
Cooperate without looking: Why we care what people think and not just what they do
Authors: Hoffman Moshe , Yoeli Erez , Nowak Martin A. 
Published: January 2015

SignificanceWhy do we trust people more when they do good without considering in detail the cost to themselves? People who avoid “looking” at the costs of good acts can be trusted to cooperate in important situations, whereas those who look cannot. We find that evolutionary dynamics can lead to cooperation without looking at costs. Our results illuminate why we attend closely to people’s motivations for doing good, as prescribed by deontological ethicists such as Kant, and, also, why we admire principled people, adhere to taboos, and fall in love.
Structure and mechanism ofStaphylococcus aureusTarM, the wall teichoic acid α-glycosyltransferase
Authors: Sobhanifar Solmaz , Worrall Liam James , Gruninger Robert J. , Wasney Gregory A. , Blaukopf Markus , Baumann Lars , Lameignere Emilie , Solomonson Matthew , Brown Eric D. , Withers Stephen G. , Strynadka Natalie C. J. 
Published: January 2015

SignificanceThis paper describes the structure ofStaphylococcus aureusTarM, an enzyme responsible for the glycosylation of wall teichoic acid that is important in pathological processes such as host immunity, phage binding, and antibiotic resistance in strains such as Methicillin-resistantS. aureus. The TarM structure is presented in an unusual ternary-like complex that features a polymeric acceptor substrate analogue and a trapped product of enzyme action, lending novel structural and mechanistic insight into the glycosylation of glycopolymers. More generally, the positioning of this product in the active site as well as the distorted conformation of its pyranose ring provide direct structural evidence for an internal substitution-like catalytic mechanism for retaining GT-B class enzymes.
Dual roles of an
Arabidopsis
ESCRT component FREE1 in regulating vacuolar protein transport and autophagic degradation
Authors: Gao Caiji , Zhuang Xiaohong , Cui Yong , Fu Xi , He Yilin , Zhao Qiong , Zeng Yonglun , Shen Jinbo , Luo Ming , Jiang Liwen 
Published: January 2015

Significance
Macroautophagy (hereafter as autophagy) involves the delivery of cytosolic materials via autophagosome upon its fusion with the endosome and lysosome/vacuole. The endosomal sorting complex required for transport (ESCRT) machinery is responsible for the formation of intraluminal vesicles (ILVs) in multivesicular bodies (MVBs) and the sorting of ubiquitinated membrane cargos into MVB ILVs for degradation. Here, we show that, in addition to regulating MVB biogenesis, the plant-specific ESCRT component FYVE domain protein required for endosomal sorting 1 (FREE1) also plays dual roles in vacuolar protein transport and autophagic degradation. FREE1 directly interacts with a plant autophagy regulator SH3 DOMAIN-CONTAINING PROTEIN2 to manipulate the autophagic degradation in plants. Thus, we demonstrate multiple functions of FREE1 and a direct link between the ESCRT machinery and autophagy process in plants.
Optical antenna enhanced spontaneous emission
Authors: Eggleston Michael S. , Messer Kevin , Zhang Liming , Yablonovitch Eli , Wu Ming C. 
Published: January 2015

Significance
Since the invention of the laser over 50 y ago, stimulated emission has been stronger and far more important than spontaneous emission, the ordinary light we are accustomed to. Indeed spontaneous emission has been looked down upon as a weak effect. Now a new science of enhanced spontaneous emission is emerging that makes spontaneous emission faster than stimulated emission. This new science depends upon the use of optical antennas to increase the spontaneous emission rate. Antennas emerged at the dawn of radio for concentrating electromagnetic energy to a small volume. Despite the importance of radio antennas, 100 y went by before optical antennas began to be used to help extract optical frequency radiation from very small sources such as dye molecules and quantum dots.