bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bowel preparation-induced changes in the gut environment facilitate pathogen and pathobiont colonization
Authors: Clayton Charlotte , McCallum Giselle , Ng Katharine , Srini Apsara , Sie Claire , Tertigas Dominique , Porter Imogen , Pepin Deanna , Fardeen Touran , Sinha Sidhartha , Surette Michael , Vallance Bruce , Tropini Carolina 
Published: August 2024

Abstract

Bowel preparation is a common procedure performed millions of times each year to clear the intestinal tract ahead of colonoscopy. During this procedure, a laxative is administered to clear the intestinal contents, which also results in the removal of the mucus layer that normally protects the underlying epithelium. While bowel preparation is generally considered safe in healthy individuals, with the microbiota typically recovering fully, it remains unclear whether pathogenic bacteria can exploit the environmental changes induced by this procedure to cause disease. In this study, we investigated the impact of bowel preparation on the susceptibility of mice to oral infection by the bacterial pathogen Salmonella enterica serovar Typhimurium. We found that post-bowel preparation, in the absence of antibiotic treatment, Salmonella robustly colonized, expanded in the gut and translocated to extraintestinal organs, while vehicle-treated mice were protected from infection. Colonization was effective at doses as low as 1,000 CFU and was independent of Salmonella motility, indicating that bowel preparation disrupts intestinal colonization resistance against this common enteric pathogen. We also discovered that pathobionts common in patients with inflammatory bowel disease (IBD) are highly resistant to osmotic perturbation, suggesting they may take advantage of bowel preparation to expand. In a human IBD microbiota mouse model, resident pathobionts translocated to extraintestinal organs post-bowel preparation, whereas this was not seen in mice with a conventional or healthy human microbiota. These results reveal the ability of pathogens and pathobionts to exploit disruptions in the gut environment and further elucidate the importance of the mucus layer and commensal microbiota in preventing enteric pathogen infection.
Hypersonic heat barrier mitigation via structured thermal armor technology
Authors: Wang Ji-Xiang , Li Jia-Xin , Zhong Mingliang , Wang Hongmei 
Published: August 2024

Abstract

Hypersonic aerodynamic heating, also known as the heat barrier, is a dangerous energy form that humans have not yet fully overcome. The heat barrier has caused numerous catastrophic accidents and damage, as well as hindering the development of aerospace engineering. To date, indirect thermal protection methods, such as regenerative cooling, film cooling, and transpiration cooling, have proven to be complex, low in cooling efficiency, and detrimental to a hypersonic vehicle's reusability, maneuverability, and cost-effectiveness (RMC). Inspired by the latest Leidenfrost delay technology, we propose a direct liquid cooling technology to mitigate the effects of the heat barrier. Our proposed aluminum alloy-based structured thermal armor (STA) with a simple liquid cooling system, demonstrates the capability to rapidly mitigate simulated aerodynamic heating-induced extreme temperatures, exceeding 1400°C. This temperature is significantly higher than the melting points of conventional aircraft skin materials, such as aluminum alloys, which typically range from 600 to 700°C. Cycling tests indicate the STA's high durability and tolerance properties. Our research demonstrates that the STA is highly compatible with current hypersonic aerospace systems, promoting aerospace RMC. We also provide scale-up design guidelines for practical hypersonic vehicles' thermal protection. The STA offers a promising solution for hypersonic aerodynamic heating, providing a more efficient, durable, and cost-effective approach to thermal protection. This technology has the potential to revolutionize the aerospace industry, enabling the development of safer and more advanced hypersonic vehicles.
Evolution of Collaborative Networks in Gastrointestinal Endoscopy: Analyzing Key Collaborations and Network Characteristics (2000-2023)
Authors: Ogasawara Naruaki 
Published: August 2024

Abstract

Aim
This study aims to analyze the evolution of collaborative networks in gastrointestinal endoscopy research from 2000 to 2023. The focus is on identifying key collaborations and evaluating the centrality of Japanese researchers within the global context.
Method
Data from 23,307 articles related to gastrointestinal endoscopy, published between 2000 and 2023, were extracted from the Web of Science (WoS) database. Network analysis was conducted using Python (version 3.10.5) in the PyCharm (software version 2022.1.3) development environment. The analysis employed macro-level metrics, including network density, clustering coefficient, components, and average distance, as well as micro-level metrics, such as degree centrality, closeness centrality, and betweenness centrality.
Result
The study revealed that the co-authorship network in gastrointestinal endoscopy research has evolved significantly over the past two decades. The network density slightly increased from 0.0003654681 in 2000–2009 to 0.00034 in 2020–2023, while the average clustering coefficient also rose, indicating stronger clustering among researchers. Key researchers, including Hassan, Cesare, and Dinis-Ribeiro, Mario, were identified as central figures in the network, maintaining high degree and closeness centrality throughout the study period. Japanese researchers, such as Fujishiro, Mitsuhiro, and Saito, Yutaka, also played a prominent role in connecting different subgroups within the network, particularly in the later years.
Conclusion
The analysis demonstrates the dynamic nature of collaborative networks in gastrointestinal endoscopy research, with Japanese researchers playing a pivotal role in global collaborations. The findings highlight the importance of these researchers in driving innovation and shaping the future of gastrointestinal endoscopy.
Effect of Indole-3-Acetic Acid, Indole-3-Butyric Acid, and 1-Naphthalene Acetic Acid on The Stem Cutting and Vegetative Growth of Lawsonia Inermis L.
Authors: Uddin Mohammed Sala , Musa Md. Abu Shale 
Published: August 2024

Abstract

Conventional methods for Lawsonia inermis L. propagation face problems like short viability, disease, pests, and unreliable seed propagation due to natural habitat conditions for large-scale production. In-vitro micropropagation also shows drawbacks in mass-scale production due to poor hardening percentage and more time requirements in the context of producing the desired amount of leaf biomass. Therefore, vegetative propagation via stem cuttings to multiply superior, healthy plants is convenient for achieving increased productivity in terms of leaf biomass. Auxins have a significant effect on the rooting and shooting of cuttings. An experiment was carried out at the Botanic Garden, University of Chittagong, in the southeastern region of Bangladesh to determine the effect of IAA, IBA, and NAA on the vegetative growth potential of stem cuttings of L. inermis following the quick dip method and completely randomized design. Results showed significant variability in root and shoot development, which influences the leaf biomass production of L. inermis cuttings. IAA, IBA, and NAA all had significant effects on shoot and root development on cuttings except in control cuttings, where cuttings developed adequate roots without hormone application. It concludes that IBA-treated cuttings could be considered as a higher amount of leaf biomass producing and more effective than IAA and NAA as propagation material for L. inermis, which may play a vital role in the herbal medicine industry as well as in economically developing countries like Bangladesh. It was recommended to investigate how IAA, IBA, and NAA, together with other plant growth regulators, affect L. inermis's capacity for roots and shooting in producing the greatest amount of leaf biomass.
L-ascorbate Alleviates Chronic Obstructive Pulmonary Disease through the EGF/PI3K/AKT Signaling Axis
Authors: Yao Ji , Zhang Li , Zhou Zezhi , Liu Jiqiang , Cheng Jie , Long Fan , Yuan Ting 
Published: August 2024

Introduction:
In this study, the molecular mechanisms through which Lascorbate
(Vitamin C) potentially treats Chronic Obstructive Pulmonary Disease
(COPD) were identified. A non-targeted metabolomics analysis revealed metabolic disorders
and significantly reduced levels of L-ascorbate in COPD patients compared to
healthy subjects.

Method:
The COPD rat model was established by exposing them to Cigarette Smoke (CS).
The L-ascorbate intervention reduced lung inflammation and histological damage in
COPD rat models. Network pharmacology analysis revealed 280 common targets between
L-ascorbic acid (drug) and COPD (disease), of which seven core targets were
MMP3, MME, PCNA, GCLC, SOD2, EDN1, and EGF. According to molecular docking
prediction, L-ascorbate had the highest affinity with EGF. Molecular dynamics simulation
indicated relatively stable EGF and L-ascorbate complexes.

Results:
The PI3K/AKT signaling pathway was significantly enriched in the Kyoto Encyclopedia
of Genes and Genomes (KEGG) functional analysis.

Conclusion:
Finally, the in vivo and in vitro experiments confirmed that L-ascorbate affected
COPD by regulating the EGF/PI3K/AKT pathway. In summary, based on network
pharmacology and molecular docking analyses, this study revealed that L-ascorbate
affects COPD development by regulating the PI3K/AKT signaling pathway
through EGF, and thus contributes to the understanding and clinical application of Lascorbate
in the treatment of COPD.
Comprehensive Analysis and Experimental Validation of TLL2 as a Potential New Prognostic Biomarker Associated with Immune Infiltration in
Lung Adenocarcinoma
Authors: Jiang Jing , Du Zhongye , Tang Haijuan , Huang Yingying , Li Dongbing , Liang Qiuli 
Published: August 2024

Background:
The precise function of Tolloid Like 2 (TLL2) remains uncertain within
the context of Lung Adenocarcinoma (LUAD).

Objective:
The primary objective of this investigation was to conduct a thorough analysis.

Methods:
To assess its diagnostic utility, data from The Cancer Genome Atlas (TCGA) database
were used to assess TLL2 expression in pan-cancer and LUAD. The study has also investigated
the correlation between TLL2 expression levels and LUAD symptoms and prognosis. Furthermore,
the study has explored possible regulatory networks involving TLL2, including its association
with immune infiltration, tumor stemness index (mRNAsi), and drug sensitivity in LUAD.
We have explored TLL2 expression in single-cell sequencing of LUAD and the genomic variation
and clinical significance of TLL2 in LUAD. The expression of TLL2 has been validated in
GSE87340 and cell lines by quantitative Real-time PCR (qRT-PCR).

Results:
An abnormal expression of TLL2 has been found in pan-cancer and LUAD. In LUAD patients,
elevated levels of TLL2 were significantly related to the T stage (p = 0.046) and the pathological
stage (p = 0.016). The expression of TLL2 in patients with LUAD was significantly associated
with poorer Overall Survival (OS) (p < 0.001). The expression of TLL2 was determined to be
an independent predictor of poorer OS (p = 0.042). TLL2 was associated with ribosome, neuroactive
ligand-receptor interaction, allograft rejection, ECM receptor interaction, asthma, porphyrin
and chlorophyll metabolism, focal adhesion, pentose and glucuronate inter-conversions, and ascorbate
and aldarate metabolism. The expression of TLL2 in LUAD was correlated with immune infiltration
and mRNAsi. The expression of TLL2 was significantly and negatively correlated with
TAK-715, XMD13-2, STF-62247, OSI-930, and EHT-1864 in LUAD. The TLL2 gene was up-
-regulated in multiple individual LUAD cells. LUAD patients with altered TLL2 had a shorter
PFS as opposed to those with unaltered TLL2. The expression of TLL2 was significantly increased
in LUAD cells.

Conclusion:
For patients with LUAD, TLL2 may serve as an immunotherapeutic target and a useful
prognosis biomarker.
Internet Gaming Disorder and Sleep Quality among Jordanian University Students: A Cross-sectional Study
Authors: Abdallat Mahmoud , Al-Sanouri Mohammad , Al-Salaymeh Suhayb , Zoubi Mohammad , Barakat Tamer , Badwan Ahmad , Alzubi Abdallah , Murshidi Rand 
Published: August 2024

Background
Internet gaming disorder is defined as “Persistent and recurrent use of the internet to engage in games, often with other players, leading to clinically significant impairment or distress.” It is a new evolving disorder that affects many life aspects; therefore, it needs further investigation among different population groups. IGD was introduced for the first time in 2013 in the fifth edition of the Diagnostic and Statistical Manual of Mental Disorders, and it suggested carrying out further research among different populations. In 2018, Gaming Disorder (GD) has officially become a type of addiction as the World Health Organization released the 11th revision of the International Classification of Diseases (ICD-11)

Aims
The objective of this study is to investigate the prevalence of internet gaming disorder (IGD) and its association with sleep quality and academic performance among Jordanian university students aged 18-26. Our literature review revealed a lack of research on this topic concerning this specific population and culture. Therefore, our study aims to contribute to the existing literature and to provide insights that can inform prevention, assessment, and treatment strategies for those affected.

Methods
A cross-sectional study design was used by employing convenience and snowball sampling; a total of 2473 participants completed an electronic self-administered questionnaire that included the Internet Gaming Disorder Scale-SF (IGDS9-SF) and Pittsburgh Sleep Quality Index (PSQI). Of these, 432 were excluded based on our criteria. Our inclusion criteria required participants to be Jordanian university students between the ages of 18 and 26, enrolled as undergraduates at Jordanian universities, and free of neurological or psychiatric conditions.

Results
The prevalence of IGD in this study was 15.2% and was more prevalent among males compared to females (p= <0.001). Poor sleep quality was reported by 64.6% of the study population and was more frequently observed in females. Age and academic achievements (GPA) were not associated with having IGD. When binary logistic regression was used, IGD (OR=1.882) positively predicted poor sleep quality.

Conclusion
Internet gaming disorder is common among Jordanian university students and is associated with poor sleep quality, and our findings have significant implications for policymakers, educators, and healthcare providers in raising awareness about the IGD and its impact on sleep quality.
REVISION ANALYSIS FACTORS THAT INHIBIT THE DEVELOPMENT OF SMALL AND MEDIUM ENTERPRISES IN THE GRAPHIC DESIGN SECTOR (CASE STUDY OF EDUCATED MILLENNIALS)
Authors: Hafidz Hafidz Ash Shalih , Khusnul Ashar  
Published: August 2024

The graphic design industry is a very promising and very flexible industry along with the development of technology and also the use of social media among the public. Departing from this, it was found that small and medium enterprises engaged in graphic design, especially millennials, caused great market competition. This has resulted in many graphic design businesses that are unable to develop or even regress. But behind the high market competition, there are still many graphic design businesses that are still able to maintain their existence or even develop. Departing from this, the purpose of this research is to determine the factors that hinder the development of graphic design businesses. There are 6 factors that can hinder business development, namely Human Resources (HR) factors, Planning Factors, Marketing and Sales Factors, Business Management Factors, Venture Capital, and Government Assistance. This study used qualitative descriptive analysis method with data collection techniques through interviews. Based on the analysis of results / findings in the field, researchers can conclude that there are 5 factors that hinder the development of graphic design businesses, especially the millennial sector, namely Human Resources (HR) factors, Planning Factors, Marketing and Sales Factors, Business Management Factors, Business Capital While government assistance is considered influential. The results of this study can be used as a basis for taking policies for graphic design business owners specifically for the millennial sector to improve their business development and can also be used as a learning for prospective entrepreneurs, especially in the field of graphic design.

Keywords: Graphic Design, MSMEs, Millennials
Pengaruh Kualitas Layanan Logistik, Citra, Merek, Dan Promosi Penjualan Terhadap Kepuasan Pelanggan
Authors: Azizulfikri Dimas 
Published: August 2024

The objective of this research is to identify the effects of logistic service quality, brand image, and sales promotion on the satisfaction of J&T Express’s customers in Jabodetabek. This explanatory research was conducted to explain the relationship between one variable and the others. Using purposive sampling, 205 people were selected as the respondents; they are people with the minimum age of eighteen years who live in Jabodetabek, who have ordered and paid for J&T Express’ service at least once, and who have made direct contacts with the logistic companies’ officer in one of its branch offices in the said region. The data was analyzed using multiple linear regression, and the hypotheses were tested using the t-test; all of which were processed in IBM SPSS Statistics 25. This study finds that logistic service quality, brand image, and sales promotion have direct and significant impacts on customer satisfaction.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh kualitas layanan logistik, citra merek, dan promosi penjualan terhadap kepuasan pelanggan J&T Express di Jabodetabek. Jenis penelitian ini adalah penelitian eksplanatif yang dilakukan guna menjelaskan hubungan variabel satu dengan variabel lainnya. Penelitian ini menggunakan sampel sebanyak 205 responden di Jabodetabek dengan teknik purposive sampling. Karakteristik sampel untuk penelitian ini adalah berusia diatas 18 tahun, berdomisili atau sedang berada di Jabodetabek, pernah memesan dan membayar layanan J&T Express setidaknya satu kali, serta pernah melakukan kontak langsung dengan petugas J&T Express di salah satu cabang J&T Express di Jabodetabek. Analisis data pada penelitian ini menggunakan Analisis Regresi Linier Berganda dan Uji Hipotesis menggunakan Uji t. Pengolahan data dilakukan melalui program IBM SPSS Statistics 25. Berdasarkan pengujian yang dilakukan, dapat ditemukan variabel Kualitas Layanan Logistik, Citra Merek, dan Promosi Penjualan memiliki pengaruh langsung dan signfikan terhadap variabel Kepuasan Pelanggan.
Faktor-Faktor Yang Memengaruhi Willingness to Subscribe Pada Layanan Subscription Video on Demand
Authors: Herita Tasya Dwi , Nugroho Dian Ari 
Published: August 2024

The objective of this study is to identify the effects of content marketing, perceived price, electronic word-of-mouth, and brand image on the willingness of Vidio users in the Special Capital Region of Jakarta to subscribe the application’s video-on-demand service. The sample of this quantitative explanatory research was selected using non-probability sampling method and purposive sampling technique. Using the criteria of people with the minimum age of eighteen years who live in the special region and who are subscribing or have subscribed services provided by Vidio within the last one year, 150 people were selected as the respondents. This research uses validity testing, reliability testing, and classical assumption testing performed in the Statistical Package for the Social Sciences (SPSS) version 29. The hypotheses were tested using F test and t-test. This study finds that the independent variables, i.e., content marketing, perceived price, and brand image significantly influence the willingness of Vidio users to subscribe its services.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh Conten Marketing, Perceived Price, Electronic Word of Mouth, dan Brand Image terhadap Willingness to Subscribe pada layanan Subscription Video on Demand (studi pada pengguna Vidio di DKI Jakarta). Jenis penelitian ini adalah explanatory research dengan metode kuantitatif. Penelitian ini menggunakan sampel sebanyak 150 responden. Pengambilan sampel menggunakan teknik non probability sampling dengan metode purposive sampling. Kriteria responden yang dibutuhkan dalam penelitian ini adalah berusia minimal 18 tahun, sedang dan/atau pernah membeli paket berlangganan pada aplikasi Vidio dalam kurung waktu 1 tahun terakhir, dan berdomisili di DKI Jakarta. Penelitian ini menggunakan program Statistical Package for the Social Sciences (SPSS) versi 29 untuk melakukan uji validitas, uji reliabilitas, dan uji asumsi klasik. Uji hipotesis dilakukan menggunakan uji t dan uji F. Dari hasil penelitian dapat disimpulkan bahwa terdapat tiga variabel dependen yaitu Content Marketing, Perceived Price, dan Brand Image yang masing-masing memiliki pengaruh secara signifikan terhadap Willingness to Subscribe pengguna terhadap layanan Vidio.