bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of Operating Parameters of Plasma Antenna
Authors: Soltani Gishini M. S. , Ganjovi A. , Taraz M. , Saeed M. 
Published: September 2015

AbstractIn this work, the operating parameters of the plasma antenna are optimized using a kinetic model based on Particle in Cell‐Monte Carlo Collisions (PIC‐MCC) method. This optimization study is performed via the investigation of variations in the operating parameters of the plasma antenna, i. e., its dimensions, background gas pressure, and the applied voltage frequency and their consequent effects on the plasma frequency, kinetic energy of electrons and plasma current density of plasma antenna. While the antenna performance is improved at higher tube lengths and applied frequencies, it is optimized at a particular tube radius. Moreover, higher background pressures have increasing effects on the plasma antenna operation. Based on this parametric study, the optimum operating parameters of the plasma antenna are established. (© 2015 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Generation and flow cytometric quality control of clinical‐scale TCRαβ/CD19‐depleted grafts
Authors: Bremm Melanie , Cappel Claudia , Erben Stephanie , Jarisch Andrea , Schumm Michael , Arendt Andreas , Bonig Halvard , Klingebiel Thomas , Koehl Ulrike , Bader Peter , Huenecke Sabine 
Published: September 2015

BackgroundThe depletion of TCRαβ+ T cells and CD19+ B cells is a graft purification method for haploidentical stem cell transplantation (HSCT) retaining stem cells, NK cells and TCRγδ+ T cells. To avoid treatment‐related occurrence of severe GvHD a precise quantification of residual TCRαβ+ T cells in the graft is of essential importance.MethodsNine stem cell grafts were purified immunomagnetically on a CliniMACS device and flow cytometric quality control (QC) was performed before and after TCRαβ/CD19‐depletion.ResultsAs a challenge a new 10‐color QC‐panel was established, which enables accurate quantification of the graft composition. The binding sites of residual TCRαβ+ T and CD19+ B cells were at least partly occupied by depletion antibodies impeding flow cytometric analysis. Based on respective controls and an assumed variation coefficient of 18%, the detection limit of residual TCRαβ T cells was 1 cell/µl. and 0.002% of CD45+ cells. Log‐depletion of TCRαβ and CD19 cells was −3.9 and −3.3, respectively. The recovery was 82.1%, 67.1% and 72.7% for stem cells, NK cells and for TCRγδ+ T cells.ConclusionsIn clinical use this method may help to improve transplantation outcome, due to the correct application of the desired stem cell and the limited T cell dose. The panel is designed for the QC following TCRαβ/CD19‐depletion but is adaptable to other depletion strategies as well. © 2015 International Clinical Cytometry Society
Prognostic value of residual pulmonary congestion at discharge assessed by lung ultrasound imaging in heart failure
Authors: Coiro Stefano , Rossignol Patrick , Ambrosio Giuseppe , Carluccio Erberto , Alunni Gianfranco , Murrone Adriano , Tritto Isabella , Zannad Faiez , Girerd Nicolas 
Published: September 2015

AimsResidual pulmonary congestion at discharge is associated with poor prognosis in heart failure (HF), but its quantification through physical examination is challenging. Ultrasound imaging of lung comets (B‐lines) could improve congestion evaluation. The aim of this study was to assess the short‐term prognostic value of B‐lines after discharge from HF hospitalisation compared with other indices of haemodynamic congestion (BNP, E/e', and inferior vena cava diameter) or clinical status (NYHA class).Methods and resultsSixty consecutive HF inpatients underwent clinical examination, echocardiography, and lung ultrasound at discharge, independently of, and in addition to routine management by the attending physicians. The median B‐line count was 8.5 (5–34). Three‐month event‐free survival for the primary endpoint (all‐cause death or HF hospitalisation) was 27 ± 10% in patients with ≥30 B‐lines and 88 ± 5% in those with <30 B‐lines (P < 0.0001). In a multivariable model, ≥30 B‐lines significantly predicted the combined endpoint (hazard ratio 5.66, 95% confidence interval 1.74–18.39, P = 0.04), along with NYHA ≥III and inferior vena cava diameter, while other indirect measures of congestion (BNP and E/e' ≥15) were not retained in the model; furthermore ≥30 B‐lines independently also predicted the secondary outcomes (HF hospitalisation and death). Importantly, B‐line addition to NYHA class and BNP was associated with improved risk classification (integrated discrimination improvement 15%, P = 0.02; continuous net reclassification improvement 65%, P = 0.03).ConclusionResidual pulmonary congestion at discharge, as assessed by a B‐line count ≥30, is a strong predictor of outcome. Lung ultrasonography may represent a useful tool to identify and monitor congestion and optimize therapy during and/or after hospitalisation for HF, which should be further validated in multicentre studies.
Iridium(I) Complexes Bearing a Noninnocent PNP‐Pincer‐Type Phosphaalkene ­Ligand: Catalytic Application in the Base‐Free N‐Alkylation of Amines with Alcohols
Authors: Chang Yung‐Hung , Tanigawa Ippei , Taguchi Hiro‐omi , Takeuchi Katsuhiko , Ozawa Fumiyuki 
Published: September 2015

AbstractA series of IrI complexes [Ir(L)(PPEP*)] [L = Cl– (3), CO (4), tBuNC (5), PMe3 (6), PPh3 (7)], coordinated with a PNP‐pincer‐type phosphaalkene ligand bearing a dearomatized pyridine ring (PPEP*), have been prepared and their catalytic properties for the dehydration/condensation of amines with alcohols has been examined. The catalytic reactions successfully proceed under base‐free conditions to give N‐alkylated amines and their dehydrogenation derivatives (imines). The product selectivity is dependent on L coordinated with Ir(PPEP*). Complexes 4 and 5 that contain π‐accepting ligands (CO, tBuNC) form N‐alkylated amines as the major products in a closed system using a nitrogen‐gas‐filled Schlenk tube. In contrast, complex 7 that contain PPh3 as L produces imines as the major products under a nitrogen‐gas flow. The reason for the selectivity change depending on L is discussed based on stoichiometric reactions using model compounds of presumed catalytic intermediates.
Synthesis of New Diketopiperazines, Thiolation to Thiodiketopiperazines, and Examination of Their ROS‐Generating Properties
Authors: Zhong Sabilla , Wandler Angela E. E. , Schepers Ute , Nieger Martin , Bräse Stefan 
Published: September 2015

AbstractA variety of new symmetrical and unsymmetrical diketopiperazines have been prepared from free amino acids by using a previously developed microwave‐assisted protocol. This included the successful incorporation of L‐pyroglutamic acid as an unusual building block. The diketopiperazines were then thiolated electrophilically to the corresponding bis(methylthio)‐ and epidithiodiketopiperazines. Initial experiments showed a promising activity towards the generation of reactive oxygen species in HeLa cells.
Synthesis of Isoxazoline Derivatives Based on Nitrile Oxide Cycloaddition of Nitroso‐Nitro‐Enamine
Authors: Kondacs László András , Pilipecz Mihály Viktor , Mucsi Zoltán , Balázs Barbara , Gáti Tamás , Nyerges Miklós , Dancsó András , Nemes Péter 
Published: September 2015

AbstractA new and stable nitroso‐nitro‐enamine reagent, providing a nitrile oxide 1,3‐dipole, has been treated with dipolarophiles in the course of 1,3‐dipolar cycloaddition reactions to give a large number of novel isoxazolyl‐pyrroline derivatives. Surprisingly, instead of the expected 2‐isoxazolyl‐dihydropyrrole cycloadducts, dihydropyrrol‐3‐one oximes were isolated as the main products in most cases. The imino‐oxime moiety was successfully transformed into 3‐aminopyrrole derivatives. The mechanism and selectivity of the reactions were also studied by experimental and theoretical methods [WB97XD/6‐311++G(2d,2p)//B3LYP/6‐311++G(2d,2p)], including the solvent effect.
Synthesis of Homo‐C‐Nucleoside Phosphoramidites and Their Site‐Specific Incorporation into Oligonucleotides
Authors: Höfler Katharina , Zimmermann Tristan , Peña Fuentes Dilver , Vogel Christian , Meier Chris 
Published: September 2015

AbstractAn efficient procedure was developed to prepare homo‐C‐nucleosides. The β‐allyl C‐glycoside of D‐ribose was transformed into the thienopyrimidine nucleoside and the benzodiazepine nucleoside of 2‐deoxy‐D‐ribose. For incorporation into oligonucleotides by solid‐phase synthesis, both derivatives were transformed into the related 3′‐phosphoramidite building blocks. These phosphoramidites were then site‐specifically incorporated into DNA oligonucleotides. The modified DNA strands were hybridized with different DNA and RNA strands, and their melting temperatures and circular dichroism spectra were studied. Interestingly, although in the case of DNA–DNA hybrids the nonnatural nucleosides caused a marked decrease in melting temperature, to levels even lower than those for mismatched hybrids, in the case of DNA–RNA hybrids very similar melting temperatures were measured for the nonnatural nucleosides and the unmodified oligonucleotides.
Epidemiology of uraemic itch: New data
Authors: Weisshaar E. 
Published: September 2015

AbstractChronic itch (CI) is a frequent and impairing symptom in haemodialysis (HD) patients. Within the last decades, worldwide variations were reported in the prevalence of itch in HD patients, ranging from 10 to 77%. Regional differences and especially the irregular pattern of itch in HD may explain why CI is still not well perceived and underestimated in HD patients. The pathogenesis is still unclear and therapy is ineffective in the majority of cases. There is a great diversity of prevalence estimates of itch used in different studies as well, as the study population greatly differs, especially when investigating CI in HD. Due to the demographic situation with a rising number of aged patients and increasing survival rates in HD, the number of HD patients will continuously grow, especially in the Western countries. Up to now, representative epidemiological data on CI in HD were missing. GEHIS (German Epidemiological Hemodialysis Itch Study) was established in 2012 as a representative prospective cross‐sectional study. Eight hundred and sixty HD patients from a randomly selected cluster sample were included. The primary outcome measures were different prevalence measures of CI. The point prevalence was 25.2%, the 12‐month prevalence was 27.2% and the lifetime prevalence of CI was 35.2%. General health status and health‐related quality of life (HRQOl) were significantly impaired in those with CI. Due to the comparability of standards in HD quality, these results can be transferred to Western countries. This study demonstrates that CI is a frequent, long‐lasting burden in HD patients affecting every fourth HD patient (point prevalence) and more than every third HD patient at some point in their lifetime.
Aliphatic Polyamine Oxidation Reaction Mechanism at Boron‐doped Microcrystalline and Ultrananocrystalline Diamond Electrodes
Authors: Witek Małgorzata , Koppang Miles D. , Swain Greg M. 
Published: September 2015

AbstractThe oxidation reaction mechanism of several aliphatic polyamines was studied using diamond electrodes with different doping levels and non‐diamond carbon impurity content. The important roles of surface boron and localized non‐diamond carbon sites in the polyamine oxidation reaction mechanism were confirmed. A well defined oxidation peak was seen for all the amines that was dependent on the onset potential for water discharge. Epox shifted negative and the ipox increased with increasing solution pH. ipox also increased with increasing boron doping level or surface boron site density. FIA‐EC analysis revealed a reproducible response for the aliphatic polyamines in a binary solvent (7/93 %) acetonitrile/borate buffer, pH 11.2. Optimum signal‐to‐background ratios for the aliphatic polyamines were seen at potentials between 650 and 670 mV.
Prevalence of Alcohol and Other Substance Use in Patients with Eating Disorders
Authors: Fouladi Farnaz , Mitchell James E. , Crosby Ross D. , Engel Scott G. , Crow Scott , Hill Laura , Le Grange Daniel , Powers Pauline , Steffen Kristine J. 
Published: September 2015

AbstractObjectiveThe major aim of this study was to investigate any association between binge eating and purging and alcohol and substance use.MethodThe Eating Disorder Questionnaire was completed by 2966 patients. Each patient was assigned to an approximate diagnostic group based on a DSM‐5‐based algorithm.ResultsPatients with bulimia nervosa (BN) used alcohol/other substances with higher frequencies compared to patients with anorexia nervosa‐restricting type (AN‐R), binge eating disorder (BED), and eating disorder not otherwise specified (EDNOS; p < 0.001). Patients with anorexia nervosa‐binge eating/purging type (AN‐BP) were more likely to use alcohol/substances than those with AN‐R [odds ratio for alcohol use: 3.58 (p < 0.01); odds ratio for substance use: 30.14 (p < 0.01)]. Higher frequencies of binge eating and purging were associated with higher frequencies of substance use.DiscussionPatients who manifest both binge eating and purging behaviour are at higher risk of substance use which may have important treatment implications. Copyright © 2015 John Wiley & Sons, Ltd and Eating Disorders Association.
Experimental and numerical investigation of the gas‐phase effectiveness of phosphorus compounds
Authors: Bouvet Nicolas , Linteris Gregory , Babushok Valeri , Takahashi Fumiaki , Katta Viswanath , Krämer Roland 
Published: September 2015

SummaryThe effectiveness of phosphorus‐containing compounds as gas‐phase combustion inhibitors varies widely with flame type. To understand this behavior, experiments are performed with dimethyl methylphosphonate (DMMP) added to the oxidizer stream of methane–air co‐flow diffusion flames (cup‐burner configuration). At low volume fraction, phosphorus (via DMMP addition) is shown to be about four times as effective as bromine (via Br2 addition) at reducing the amount of CO2 required for extinguishment; however, above about 3000 μL/L to 6000 μL/L, the marginal effectiveness of DMMP is approximately zero. In contrast, the diminished effectiveness does not occur for Br2 addition. To explore the role of condensation of active phosphorus‐containing compounds to the particles, laser‐scattering measurements are performed. Finally, to examine the behavior of the flame stabilization region (which is responsible for extinguishment), premixed burning velocity simulations with detailed kinetics are performed for DMMP addition to methane–air flames. Analyses of the numerical results are performed to understand the variation in the inhibition mechanism with temperature, agent loading, and stoichiometry, to interpret the loss of effectiveness for DMMP in the present experiments. Copyright © 2015 John Wiley & Sons, Ltd.
Increased visual cortical thickness in sight‐recovery individuals
Authors: Guerreiro Maria J.S. , Erfort Maria V. , Henssler Jonathan , Putzar Lisa , Röder Brigitte 
Published: September 2015

AbstractIndividuals who are born blind due to dense bilateral cataracts and who later regain vision due to cataract surgery provide a unique model to evaluate the effect of early sensory experience in humans. In recent years, several studies have started to assess the functional consequences of early visual deprivation in these individuals, revealing a number of behavioral impairments in visual and multisensory functions. In contrast, the extent to which a transient period of congenital visual deprivation impacts brain structure has not yet been investigated. The present study investigated this by assessing cortical thickness of occipital areas in a group of six cataract‐reversal individuals and a group of six age‐matched normally sighted controls. This analysis revealed higher cortical thickness in cataract‐reversal individuals in the left calcarine sulcus, in the superior occipital gyrus and in the transverse occipital sulcus bilaterally. In addition, occipital cortical thickness correlated negatively with behavioral performance in an audio–visual task for which visual input was critical, and positively with behavioral performance in auditory tasks. Together, these results underscore the critical role of early sensory experience in shaping brain structure and suggest that increased occipital cortical thickness, while potentially compensatory for auditory sensory processing, might be maladaptive for visual recovery in cases of sight restoration. Hum Brain Mapp 36:5265–5274, 2015. © 2015 Wiley Periodicals, Inc.
First Examples of the Atherton–Todd‐Like Reaction in the Absence of Bases
Authors: Trofimov Boris A. , Gusarova Nina K. , Volkov Pavel А. , Ivanova Nina I. , Khrapova Kseniya O. 
Published: September 2015

ABSTRACTOxidative cross‐coupling between secondary phosphine chalcogenides and alcohols or phenols proceeds in CCl4 medium at 80–82°C to afford phosphinoсhalcogenоic O‐esters in up to 92% yield. These couplings represent first examples of the Atherton–Todd‐like reaction occurring in the absence of bases.
Evaluating the use of plerixafor in stem cell mobilisation – an economic analysis of thePHANTASTICtrial
Authors: Martin Antony P. , Richards Sarah , Haycox Alan , Houten Rachel , McLeod Claire , Braithwaite Barbara , Clark Jack O. , Bell Joanne , Clark Richard E. 
Published: September 2015

Plerixafor is an effective haematopoietic stem cell mobilising agent in candidates for autologous transplantation, including patients with myeloma and lymphoma. Here we compare 98 plerixafor recipients in the PHANTASTIC trial with 151 historic controls mobilised by conventional chemotherapy (each with granulocyte colony‐stimulating factor, G‐CSF). Seventy (71.4%) plerixafor‐mobilised patients achieved the composite primary endpoint of ≥4 × 106CD34+ cells kg−1in ≤2 aphereses and no clinically significant neutropenia, compared to 48 (31.8%) historic controls (P < 0.001), and this significant advantage was maintained in scenario analyses testing components of this composite endpoint. A patient‐level cost analysis was undertaken for 249 patients, which included the cost of remobilising patients where initial mobilisation had failed. Combined mean treatment cost for plerixafor mobilised patients was £12,679 compared with £11,694 for historical controls. However, plerixafor produces an average saving of £3,828 per lymphoma patient but average cost increase by £5,245 per myeloma patient. The present data demonstrate cost‐effectiveness for plerixafor as a first line mobilisation agent, certainly for lymphoma patients, where substantial resource savings and achievement of the primary endpoint are likely. J. Clin. Apheresis 31:434–442, 2016. © 2015 Wiley Periodicals, Inc.
Nicotine‐Mediated Ca2+‐Influx Induces IL‐8 Secretion in Oral Squamous Cell Carcinoma Cell
Authors: Tsunoda Kou , Tsujino Ichiro , Koshi Ryosuke , Sugano Naoyuki , Sato Shuichi , Asano Masatake 
Published: September 2015

ABSTRACTCigarette smoking is one of the most important risk factors for the development of various diseases. Nicotine is the most extensively investigated component of cigarette smoke, and a comprehensive analysis of the genes induced by nicotine stimulation revealed that interleukin‐8 (IL‐8) was induced in oral squamous cell carcinoma cell (OSCC). Based on this background, the signaling mechanisms of nicotine‐mediated IL‐8 induction in OSCC was investigated. Augmented IL‐8 secretion by Ca9‐22 cells was blocked by the NF‐κB inhibitor L‐1‐4′‐tosylamino‐phenylethyl‐chloromethyl ketone (TPCK) and the nicotinic acetylcholine receptor (nAChR)‐specific inhibitor α‐bungarotoxin (αBtx). The downstream signaling pathway was further examined by pre‐incubating the cells with inhibitors against mitogen‐activated protein kinase (MEK), protein kinase C (PKC), and Ca2+/calmodulin‐dependent kinase II (CaMK II). Only the CaMK II inhibitor was found to exert an inhibitory effect on nicotine‐mediated IL‐8 secretion. Pre‐treatment of the Ca9‐22 cells with the Ca2+ chelator BAPTA‐AM drastically inhibited IL‐8 secretion. Although nicotine stimulation induced the phosphorylation of the NF‐κB p65 subunit, pre‐treatment with BAPTA‐AM was found to inhibit this activity significantly. CaMK II‐dependent p65 phosphorylation was confirmed by pre‐incubation of the cells with CaMK II inhibitor. The results from this study indicate that the binding of nicotine to nAChR induces Ca2+ influx, which results in the activation and phosphorylation of CaMK II and NF‐κB p65, respectively. Nicotine‐mediated IL‐8 induction should be a trigger for the initiation of various diseases. J. Cell. Biochem. 117: 1009–1015, 2016. © 2015 Wiley Periodicals, Inc.
DNA origami and biotechnology applications: a perspective
Authors: Chandrasekaran Arun Richard 
Published: September 2015

AbstractThe use of DNA as a material has opened up new possibilities in the field of nanobiotechnology. Specifically, DNA origami – a technique in which one long single‐stranded DNA scaffold is folded into nanoscale shapes and structures using hundreds of short 'staple' oligonucleotides – has contributed to new innovations within this field. Nanostructures created using DNA origami have found use in applications such as biosensing, triggered drug delivery, enzyme cascades and biomolecular analysis platforms. The unmatched features of DNA nanostructures such as cell permeability, biocompatibility, and spatial positioning have contributed to DNA origami playing an important role in the development of materials for biotechnology applications. © 2015 Society of Chemical Industry
Effects of particle size and binding affinity for small interfering RNA on the cellular processing, intestinal permeation and anti‐inflammatory efficacy of polymeric nanoparticles
Authors: Cheng Wenyue , Tang Cui , Yin Chunhua 
Published: September 2015

AbstractBackgroundSilencing of excessive secreted tumour necrosis factor (TNF)‐α from macrophages might be an effective therapy of ulcerative colitis (UC), which acquires improvements on small interfering RNA (siRNA) delivery vectors. Thus, in the present study, the effects of particle size and binding affinity of four polymeric nanoparticles on siRNA delivery for the treatment of UC were evaluated.MethodsGalactosylated trimethyl chitosan‐cysteine (GTC) nanoparticles of varying particle size and binding affinity for siRNA were prepared and TNF‐α siRNA was encapsulated. Their cellular transport was investigated in murine macrophages and Caco‐2 cell monolayers were utilized to analysis the intestinal permeation. Finally, in vivo anti‐inflammatory efficacy was assessed in a mouse model of UC.ResultsAlthough marginal effects of particle size on the in vitro gene silencing efficiency were detected, GTC nanoparticles with a particle size of 450 nm and stronger binding affinity for siRNA showed reduced intestinal epithelial permeability and enhanced in vivo anti‐inflammatory efficacy compared to those with a particle size of 200 nm. By contrast, the delivery processes were significantly affected by the binding affinity for siRNA, where smaller GTC nanoparticles (200 nm) with moderate siRNA binding strength exhibited remarkable cytoplasmic distribution and sufficient intracellular release of siRNA, as well as a sustained in vitro and in vivo gene silencing effect.ConclusionsNanoparticles with a particle size of 450 nm or balanced binding affinity for siRNA might be preferable for the treatment of ulcerative colitis. Copyright © 2015 John Wiley & Sons, Ltd.