bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐time monitoring of high‐gravity corn mash fermentation using in situ raman spectroscopy
Authors: Gray Steven R. , Peretti Steven W. , Lamb H. Henry 
Published: January 2013

AbstractIn situ Raman spectroscopy was employed for real‐time monitoring of simultaneous saccharification and fermentation (SSF) of corn mash by an industrial strain of Saccharomyces cerevisiae. An accurate univariate calibration model for ethanol was developed based on the very strong 883 cm−1 C–C stretching band. Multivariate partial least squares (PLS) calibration models for total starch, dextrins, maltotriose, maltose, glucose, and ethanol were developed using data from eight batch fermentations and validated using predictions for a separate batch. The starch, ethanol, and dextrins models showed significant prediction improvement when the calibration data were divided into separate high‐ and low‐concentration sets. Collinearity between the ethanol and starch models was avoided by excluding regions containing strong ethanol peaks from the starch model and, conversely, excluding regions containing strong saccharide peaks from the ethanol model. The two‐set calibration models for starch (R2 = 0.998, percent error = 2.5%) and ethanol (R2 = 0.999, percent error = 2.1%) provide more accurate predictions than any previously published spectroscopic models. Glucose, maltose, and maltotriose are modeled to accuracy comparable to previous work on less complex fermentation processes. Our results demonstrate that Raman spectroscopy is capable of real time in situ monitoring of a complex industrial biomass fermentation. To our knowledge, this is the first PLS‐based chemometric modeling of corn mash fermentation under typical industrial conditions, and the first Raman‐based monitoring of a fermentation process with glucose, oligosaccharides and polysaccharides present. Biotechnol. Bioeng. 2013; 110: 1654–1662. © 2013 Wiley Periodicals, Inc.
Nonsymmetrical Dithienylethenes Bearing Dithieno[3,2‐b:2′,3′‐d]thiophene Units with Photochromic Performance in the Crystalline Phase
Authors: Wang Hongke , Lin Hui , Xu Wei , Zhu Daoben 
Published: January 2013

AbstractFour novel nonsymmetrical photochromic diarylethene compounds containing dithieno[3,2‐b:2′,3′‐d]thiophene units were designed and synthesized to investigate their photochromic properties. All these molecules adopt a photoactive antiparallel conformation in single crystals, as revealed by X‐ray crystallographic analysis, and exhibit excellent photochromism in solution as well as in the crystalline phase.
Increased extracellular matrix and proangiogenic factor transcription in endothelial cells after cocultivation with primary human osteoblasts
Authors: Simunovic F. , Steiner D. , Pfeifer D. , Stark G.B. , Finkenzeller Günter , Lampert F. 
Published: January 2013

AbstractThe most promising strategies in bone engineering have concentrated on providing sufficient vascularization to support the newly forming tissue. In this context, recent research in the field has focused on studying the complex interactions between bone forming and endothelial cells. Our previous work has demonstrated that direct contact cocultivation of human umbilical vein endothelial cells (HUVECs) with primary human osteoblasts (hOBs) induces the osteogenic phenotype and survival of hOBs. In order to investigate the mechanisms that lead to this effect, we performed microarray gene expression profiling on HUVECs following cocultivation with hOBs. Our data reveal profound transcriptomic changes that are dependent on direct cell contact between these cell populations. Pathway analysis using the MetaCore™ platform and literature research suggested a striking upregulation of transcripts related to extracellular matrix and cell‐matrix interactions. Upregulation of a number of major angiogenetic factors confirms previous observations that HUVECs enter a proangiogenic state upon cocultivation with osteoblasts. Interestingly, the downregulated transcripts clustered predominantly around cell cycle‐related processes. The microarray data were confirmed by quantitative real‐time RT‐PCR on selected genes. Taken together, this study provides a platform for further inquiries in complex interactions between endothelial cells and osteoblasts. J. Cell. Biochem. 114: 1584–1594, 2013. © 2013 Wiley Periodicals, Inc.
Truncated thioredoxin (Trx‐80) promotes pro‐inflammatory macrophages of the M1 phenotype and enhances atherosclerosis
Authors: Mahmood Dler Faieeq Darweesh , Abderrazak Amna , Couchie Dominique , Lunov Oleg , Diderot Vimala , Syrovets Tatiana , Slimane Mohamed‐Naceur , Gosselet Fabien , Simmet Thomas , Rouis Mustapha , El Hadri Khadija 
Published: January 2013

AbstractVascular cells are particularly susceptible to oxidative stress that is believed to play a key role in the pathogenesis of cardiovascular disorders. Thioredoxin‐1 (Trx‐1) is an oxidative stress‐limiting protein with anti‐inflammatory and anti‐apoptotic properties. In contrast, its truncated form (Trx‐80) exerts pro‐inflammatory effects. Here we analyzed whether Trx‐80 might exert atherogenic effects by promoting macrophage differentiation into the M1 pro‐inflammatory phenotype. Trx‐80 at 1 µg/ml significantly attenuated the polarization of anti‐inflammatory M2 macrophages induced by exposure to either IL‐4 at 15 ng/ml or IL‐4/IL‐13 (10 ng/ml each) in vitro, as evidenced by the expression of the characteristic markers, CD206 and IL‐10. By contrast, in LPS‐challenged macrophages, Trx‐80 significantly potentiated the differentiation into inflammatory M1 macrophages as indicated by the expression of the M1 cytokines, TNF‐α and MCP‐1. When Trx‐80 was administered to hyperlipoproteinemic ApoE2.Ki mice at 30 µg/g body weight (b.w.) challenged either with LPS at 30 µg/30 g (b.w.) or IL‐4 at 500 ng/30 g (b.w.), it significantly induced the M1 phenotype but inhibited differentiation of M2 macrophages in thymus and liver. When ApoE2.Ki mice were challenged once weekly with LPS for 5 weeks, they showed severe atherosclerotic lesions enriched with macrophages expressing predominantly M1 over M2 markers. Such effect was potentiated when mice received daily, in addition to LPS, the Trx‐80. Moreover, the Trx‐80 treatment led to a significantly increased aortic lesion area. The ability of Trx‐80 to promote differentiation of macrophages into the classical proinflammatory phenotype may explain its atherogenic effects in cardiovascular diseases. J. Cell. Physiol. 228: 1577–1583, 2013. © 2013 Wiley Periodicals, Inc.
Macrophages derived from septic mice modulate nitric oxide synthase and angiogenic mediators in the heart
Authors: de la Torre Eulalia , Hovsepian Eugenia , Penas Federico N. , Dmytrenko Ganna , Castro María E. , Goren Nora B. , Sales Maria E. 
Published: January 2013

AbstractMacrophages (Mps) can exert the defense against invading pathogens. During sepsis, bacterial lipopolisaccharide (LPS) activates the production of inflammatory mediators by Mps. Nitric oxide synthase (NOS) derived‐nitric oxide (NO) is one of them. Besides, Mps may produce pro‐angiogenic molecules such as vascular endothelial growth factor‐A (VEGF‐A) and metalloproteinases (MMPs). The mechanisms involved in the cardiac neovascular response by Mps during sepsis are not completely known. We investigated the ability of LPS‐treated Mps from septic mice to modulate the behavior of cardiac cells as producers of NO and angiogenic molecules. In vivo LPS treatment (0.1 mg/mouse) increased NO production more than fourfold and induced de novo NOS2 expression in Mps. Immunoblotting assays also showed an induction in VEGF‐A and MMP‐9 expression in lysates obtained from LPS‐treated Mps, and MMP‐9 activity was detected by zymography in cell supernatants. LPS‐activated Mps co‐cultured with normal heart induced the expression of CD31 and VEGF‐A in heart homogenates and increased MMP‐9 activity in the supernatants. By immunohistochemistry, we detected new blood vessel formation in hearts cultured with LPS treated Mps. When LPS‐stimulated Mps were co‐cultured with isolated cardiomyocytes in a transwell assay, the expression of NOS2, VEGF‐A and MMP‐9 was induced in cardiac cells. In addition, MMP‐9 activity was up‐regulated in the supernatant of cardiomyocytes. The latter was due to NOS2 induction in Mps from in vivo LPS‐treated mice. In conclusion LPS‐treated Mps are inducers of inflammatory/angiogenic mediators in cardiac cells, which could be triggering neovascularization, as an attempt to improve cardiac performance in sepsis. J. Cell. Physiol. 228: 1584–1593, 2013. © 2013 Wiley Periodicals, Inc.
Tree‐ring‐based temperature reconstruction for the northern Greater Higgnan Mountains, China, since A.D. 1717
Authors: Zhang Tongwen , Yuan Yujiang , Wei Wenshou , Yu Shulong , Zhang Ruibo , Shang Huaming , Chen Feng , Fan Zi'ang , Qin Li 
Published: January 2013

AbstractIn this paper, ring‐width chronologies of pine trees (Pinus sylvestris var. mongolica) from one sampling site in the northern Greater Higgnan Mountains, China, were constructed. The results of growth‐climate responses show that mean temperature is the limiting factor affecting radial growth of pine trees in the study area. Consequently, mean temperature from May to October from 1717 to 2008 has been reconstructed using the standard chronology. For the calibrated period (1957‐2008), the explained variance of the reconstruction is 57%. The characteristics of the reconstruction expose that mean temperature has increased since the 1970s, and the decade 2000s and 1990s are also ranked as the warm decades on record. However, this period from 1970s to now is not exceptional within the past 300 years. By applying an 11‐year moving average to the reconstruction, three warm periods and three cold periods are evident. The warm and cold periods of the reconstructed mean temperature correspond well with other reconstructions. Power spectral and wavelet analysis demonstrated the existence of significant ∼70‐ and ∼100‐year cycles of variability. Furthermore, the reconstruction and North Atlantic Oscillation Index showed a significant positive correlation (r = 0.34, n = 136, p < 0.0001). Copyright © 2012 Royal Meteorological Society
Capsule function following anterior dislocation: Implications for diagnosis of shoulder instability
Authors: Rainis Carrie A. , Browe Daniel P. , McMahon Patrick J. , Debski Richard E. 
Published: January 2013

AbstractDuring shoulder dislocation, the glenohumeral capsule undergoes non‐recoverable strain, leading to joint instability. Clinicians use physical exams to diagnose injury and direct repair procedures; however, they are subjective and do not provide quantitative information. Our objectives were to: (1) determine the relationship between capsule function following anterior dislocation and non‐recoverable strain; and (2) identify joint positions at which physical exams can be used to detect non‐recoverable strain in specific capsule regions. Physical exams were simulated at three joint positions including external rotation (ER) using robotic technology before and after anterior dislocation. The resulting joint kinematics, strain distribution in the capsule, and non‐recoverable strain were determined. Following dislocation, anterior translation increased by as much as 48% (0° ER: p = 0.03; 30° ER: p = 0.03; 60° ER: p < 0.01). Capsule sub‐regions with less non‐recoverable strain required more ER to detect differences in the strain ratios between the intact and injured joint. Strain ratio changes on the humeral side of the posterior axillary pouch (0.31 ± 0.32) were significant at all joint positions (0° ER: p = 0.03; 30° ER: p = 0.048; 60° ER: p = 0.04), whereas strain ratio differences on the humeral side of the anterior axillary pouch (0.18 ± 0.21) were significant only at 60° of ER (p = 0.03). Therefore, standardizing physical exams for joint position could help surgeons identify specific locations of non‐recoverable strain that may have been ignored. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 962–968, 2013
Biological characteristics of adult degenerative nucleus pulposus cells in a three‐dimensional microcarrier stirring culture system
Authors: Ning Bin , Liu Haifei , Gong Weiming , Jiang Jianhao , Hu Yougu , Yang Shang‐You 
Published: January 2013

AbstractA major problem in reconstructing degenerative intervertebral discs is to obtain sufficient nucleus pulposus (NP) seeding cells with normal physiologic functions. The current study adopted a three‐dimensional microcarrier culture system for massive cell expansion and evaluated the biological characteristics and physiological functions of the propagated adult degenerative NP cells. Isolated adult NP cells were cultured in either microcarrier stirring culturing system or traditional monolayer cultivation. The growth characteristics, proliferation, extracellular matrix secretion, and apoptosis potential were examined to evaluate the different features of the two cultivation methods. Compared to the monolayer cultivation system, the adhesion time of NP cells in the three‐dimensional microcarrier culture system appeared longer with relatively transient stable growth period. MTT and 3H‐TdR assays suggested significantly elevated proliferation and higher thymidine incorporation rates in cells from microcarrier system compare to cells in the monolayer system at the exponential growth phase (p < 0.05). Western blot data complimented the immunostaining results that the NP cells in the microcarrier system expressed significantly more protein levels of both type collagens at the exponential growth phase than that in the monolayer system (p < 0.05). Further, significantly more 35S labeled proteoglycan incorporation was noticed in the cells on the microcarriers at both the stable growth and the exponential growth phases (p < 0.05 and p < 0.01). In conclusion, the three‐dimensional microcarrier stirring culture system provides a means of fast and massive propagation of NP seeding cells which maintain their normal physiological characteristics and functions. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 858–863, 2013
Outcomes among patients treated for gastric adenocarcinoma during the last decade
Authors: Bringeland E.A. , Wasmuth H.H. , Johnsen G. , Johnsen T.B. , Juel I.S. , Mjønes P. , Uggen P.E. , Ystgaard B. , Grønbech J.E. 
Published: January 2013

AbstractObjectivesTo evaluate the outcomes among patients treated for gastric adenocarcinoma in a referral hospital, and to identify possible trends during the last decade.MethodsAll patients evaluated for gastric adenocarcinoma during the period 1999–2009 were included.ResultsOf 397 patients, 52% were curatively resected. Crude 5‐year survival for the first 6 years period was 38.7% (CI 29.5–47.9), for the last 5 years, 49.2% (CI 38.8–59.6). Time period (P = 0.013), age (P < 0.001) and disease stage (P < 0.001), were significant predictors of long‐term survival rates. Among curatively resected, in‐hospital mortality was reduced from 8.5% in the first period to 2.0% in the last one (P = 0.037). There was a significant increase in the use of primary stents from the first to the last period (P = 0.006), paralleled by a significant reduction in the number of explorative laparotomies or bypass procedures (P < 0.001).ConclusionsDuring the last decade, long‐term survival rates improved among patients curatively resected for gastric adenocarcinoma, and in‐hospital mortality was substantially reduced. For patients in a non‐curative situation, there was a significant shift from explorative laparotomies or bypass procedures to primary use of stents. J. Surg. Oncol. 2013;107:752–757. © 2013 Wiley Periodicals, Inc.
Nutrition intervention to decrease symptoms in patients with advanced heart failure
Authors: Lennie Terry A. , Moser Debra. K. , Biddle Martha J. , Welsh Darlene , Bruckner Geza G. , Thomas D. Travis , Rayens Mary Kay , Bailey Alison L. 
Published: January 2013

AbstractFor a majority of patients with advanced heart failure, there is a need for complementary, non‐pharmacologic interventions that could be easily implemented by health care providers to provide palliative care. Three major pathologic pathways underlying heart failure symptoms have been identified: fluid overload, inflammation, and oxidative stress. Prior research has demonstrated that three nutrients‐sodium, omega‐3 fatty acids, and lycopene‐can alter these pathologic pathways. Therefore, the purposes of this study are to test the effects of a 6‐month nutrition intervention of dietary sodium reduction combined with supplementation of lycopene and omega‐3 fatty acids on heart failure symptoms, health‐related quality of life, and time to heart failure rehospitalization or all‐cause death. The aims of this double blind‐placebo controlled study are (1) to determine the effects of a 6‐month nutrition intervention on symptom burden (edema, shortness of air, and fatigue) and health‐related quality of life at 3 and 6 months, and time to heart failure rehospitalization or all‐cause death over 12 months from baseline; (2) compare dietary sodium intake, inflammation, and markers of oxidative stress between the nutrition intervention group and a placebo group at 3 and 6 months; and (3) compare body weight, serum lycopene, and erythrocyte omega‐3 index between the nutrition intervention group and a placebo group at 3 and 6 months. A total of 175 patients with advanced heart failure will be randomized to either the nutrition intervention or placebo group. © 2013 Wiley Periodicals, Inc. Res Nurs Health 36:120–145, 2013
The influence of social‐developmental context and nurse visitation intervention on self‐agency change in unmarried adolescent mothers
Authors: DeSocio Janiece E. , Holland Margaret L. , Kitzman Harriet J. , Cole Robert E. 
Published: January 2013

AbstractPregnancy among unmarried adolescents has been linked to negative personal control beliefs. In contrast, self‐agency beliefs about control over future possibilities have been linked to delay in subsequent childbearing. In this secondary analysis, we examined factors associated with self‐agency change in 429 unmarried adolescent mothers from intervention and control groups of a nurse home visitation study. Adolescent mothers who participated in a sustained relationship with a nurse made greater gains in self‐agency than did control group mothers (p = .034). Adolescents with lower cognitive ability who were behind their age‐appropriate grade level in school made the greatest self‐agency gains. © 2013 Wiley Periodicals, Inc. Res Nurs Health 36:158–170, 2013
Hospital‐based home care for children with cancer: Feasibility and psychosocial impact on children and their families
Authors: Hansson Helena , Kjærgaard Hanne , Johansen Christoffer , Hallström Inger , Christensen Jane , Madsen Marianne , Schmiegelow Kjeld 
Published: January 2013

AbstractBackgroundTo assess the feasibility and psychosocial impact of a hospital‐based home care (HBHC) program for children with cancer.ProcedureA HBHC program was carried out with 51 children (0–18 years) with cancer to assess its feasibility in terms of satisfaction, care preferences, safety, and cost. A controlled trial was conducted to assess children's health‐related quality of life (HRQOL) using the parent‐reported and self‐reported PedsQL Generic Core Scale and PedsQL Cancer Module, and the psychosocial impact on the family by PedsQL Family Impact Module comprising a subsample of 28 children and 43 parents in the home care group, and 47 children and 66 parents receiving standard hospital care.ResultsAll parents in the HBHC program were satisfied and preferred home care. There were no serious adverse events associated with HBHC, and costs did not increase. When adjusting for age, gender, diagnosis and time since diagnosis, we found significant higher HRQOL scores in parent‐reported physical health (P = 0.04; 95% confidence interval (CI): −0.2–19.5) and worry (P = 0.04; 95% CI: −0.4–20.6) in the home‐care group indicating better physical health and less worry for children in the home‐care group. No significant difference was found in the Family Impact Module.ConclusionThis study indicates that HBHC is a feasible alternative to hospital care for children with cancer, and is greatly preferred by parents. Specific aspects of children's HRQOL may be improved with HBHC and the psychosocial burden on the family does not increase. Pediatr Blood Cancer 2013; 60: 865–872. © 2013 Wiley Periodicals, Inc.
High‐grade prostate cancer and biochemical recurrence after radical prostatectomy among men using 5α‐reductase inhibitors and alpha‐blockers
Authors: Murtola Teemu J. , Kujala Paula M. , Tammela Teuvo L.J. 
Published: January 2013

AbstractBACKGROUNDTwo clinical trials have shown that users of 5α‐reductase inhibitors finasteride and dutasteride (5‐ARIs) have reduced overall prostate cancer risk, while the proportion of high‐grade tumors is increased. We studied tumor characteristics, risk of biochemical recurrence and mortality after radical prostatectomy in 5‐ARI and alpha‐blocker users.METHODSThe study cohort consisted of 1,315 men who underwent radical prostatectomy at the Tampere University Hospital during 1995–2009. Biochemical relapse was defined as serum PSA ≥ 0.2 ng/ml after the operation. Information on mortality and medication purchases was obtained from national registries. Cox proportional regression was used to analyze hazard ratios (HRs) and 95% confidence intervals (95% CI) of biochemical relapse and death.RESULTSThe proportion of high‐grade (Gleason 7–10) tumors was significantly elevated among men who had used 5‐ARIs for 4 years or longer compared to the non‐users (83.3% vs. 53.3%, respectively). Survival curves for biochemical relapse‐free survival differed between long‐term and short‐term 5‐ARI users, but the hazard ratio remained statistically non‐significant. Risk of biochemical recurrence was elevated among alpha‐blocker users (HR 1.68, 95% CI 1.37–2.06), but in sensitivity analyses this was evident only in men using alpha‐blockers after prostatectomy. Mortality was not associated with medication usage.CONCLUSIONSLong‐term users of finasteride or dutasteride had more often high‐grade prostate cancer. Our results suggest also worse progression‐free survival. The association between risk of biochemical recurrence and post‐operative alpha‐blocker usage suggests that voiding or storage symptoms after prostatectomy may predict biochemical relapse. Prostate 73: 923–931, 2013. © 2013 Wiley Periodicals, Inc.