bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors Affecting Growth of Sulfate-Reducing Bacteria Isolated from Tropical Soil
Authors: Ilori M. O. , Okonkwo A. M. , Bamidele M. 
Published: February 2015

Abstract
Sulfate-reducing bacteria (SRB) were isolated from soils around corroded pipelines and tanks. High num­bers of the organisms occurred in areas closest to the corroded tanks and pipelines. Morphological types cor­ responding to rod, spirilloid, vibriod and coccoid were encountered. All the organisms utilized lactate as carbon and energy source. None could grow at temperatures higher than 40 °C. All the isolates grew at 1% (w/v) NaCl while none could grow at 8% (w/v) NaCl. All the isolates grew at pH 7 .0 -7 .5 . Growth was not recorded at pH below 5.5 and above 8.0. These factors may be useful in manipulating tropical soil environments to reduce activities of SRB in corrosion of pipelines and tanks.
Effect of Extreme Temperature on Quantum Yield of Fluorescence and Membrane Leakage of the Canarian Endemic Pine (Pinus canariensis)
Authors: Peters Juliane , Jiménez M. Soledad , Morales Domingo 
Published: February 2015

The effect of extreme temperature on the quantum yield of fluorescence and membrane leakage of Pinus canariensis growing in 5 stands at different altitudes and orientation in Tenerife (Canary Islands, Spain) was determined. Needles were collected from the field and transferred to the laboratory where they were kept in a closed chamber with water-saturated air overnight. Then they were exposed for 30 minutes in plastic bags in a water bath at temperature treatments with steps of 2K between 56 °C and -24 °C and the effect was determined immediately and 24 hours after the treatment by chlorophyll fluorescence and electrolyte leakage.
Needles presented incipient damage at temperatures ranging from -5 to -10 °C depending on the altitude and orientation of the stand. The results were more evident when the measurements were done 24 hours after the cold treatment and values were consistent with the electrolyte leakage results. Different resistance to high temperature depending on the altitude and orientation was also found, varying the temperature for incipient damage from 42 to 44 °C detected with the fluorescence parameters but not with the leakage of electrolytes which was not found until 50 °C. The amplitude of thermal limits for photosynthetic efficiency alteration in needles of P. canariensis was relatively narrow and similar to that of evergreen Canarian laurel forest trees
Does Elevated CO2 Protect Grain Yield of Wheat from the Effects of Ozone Stress?
Authors: Donnelly Alison , Jones Michael B. , Burkeb James I. , Schnieders Bert 
Published: February 2015

This study has investigated the effects of elevated CO2 and elevated O3, both singly and in combination, on the yield of spring wheat (Triticum aestivum L., cv. Minaret). Plants were grown in open-top chambers and exposed to three CO2 concentrations (ambient, 510 and 680 ppmv) and two O3 concentrations (ambient and ambient +50 or +90 ppbv) either from anthesis onwards or for the full growing season.
To date, experiments that have investigated the interactive effects of these gases have shown a variety of responses, ranging from an amelioration of the damaging effects of high O3 to a greater sensitivity to O3 at elevated CO2. The effects on grain yield and yield com ponents were determined. Our results confirm that elevated CO2 provides some protection to a wheat crop against the damaging effects of O3 on grain yield. However, the level of protection varies from one growing season to the next and also appears to be related particularly to the timing of exposure to elevated O3
A Comparative Study of Stationary Phase for Separation of Biflavonoids from Rheedia gardneriana Using Column Chromatography
Authors: Rodrigues Clóvis A. , Oliveira Ana E. , Schürmann da Silva Ana F. , Filho Valdir Cechinel , Guimarães Claudio L. , Yunes Rosendo A. , Delle Monache Franco 
Published: February 2015

Abstract
This paper describes a comparative study by using different chromatographic supports (silica gel, chitin and chitosan) to separate biflavonoids from Rheedia gardneriana by column chromatography. The results indicated that chitin can be used as alternative method, but the yield of the compounds is lower than when silica gel is employed. In contrast, chitosan is not a good chromatographic support for the separation of the biflavonoids under the same experimental conditions.
Novel Resorcinol Derivatives from Lasiodiplodia theobromae
Authors: Yang Qing , Asai Maki , Yoshihara Teruhiko 
Published: February 2015

Abstract
Two novel resorcinol derivatives were isolated from the mycelium of Lasiodiplodia theobromae IFO 31059 as potato micro-tuber inducing compounds. Their structures were established by spectroscopic methods as ethyl (6′R)-2,4-dihydroxy-6-(6′-hydroxyheptyl)benzoate (8) and isobutyl (6′R)-2.4-dihydroxy-6-(6′-hydroxyheptyl)benzoate (9). The absolute stereochemistry of the asymmetric center of (8) was determined by an advanced Mosher method.
Resistance Induction in Plants by a Brassinosteroid-Containing Extract of Lychnis viscaria L.
Authors: Roth Udo , Friebe Annette , Schnabl Heide 
Published: February 2015

Abstract
Resistance inducing effects of an extract of Lychnis viscaria L. seeds, containing different brassinosteroids (BR), were investigated. Application of aqueous solutions in concentrations from 0.5 to 10 mg/l (dry wt. of extract) resulted in an enhanced resistance of tobacco, cucumber and tomato to viral and fungal pathogens of up to 36% compared with water-treated control plants. No direct anti-fungal effects in mycelium growth assays with Phytophthora infestans could be observed. After treatment and inoculation with powdery mildew a stimulation of different PR-proteins (ca. + 20% for peroxidase, + 30% for chitinase and up to + 68% for β-1,3-glucanase) in cucumber was found. A chitinase in gel-electrophoresisassay showed a stronger induction of a distinct isoform under the same conditions.
Time course of peroxidase induction and changes of apoplastic protein patterns revealed by SDS-PAGE indicated an earlier triggering of defence responses after plant-extract treatment and pathogen attack, probably being responsible for the increased resistance. Involvement of the brassinosteroids in the plant extract is discussed to elicit or mediate the activation of defence-mechanisms.
Chloroplast Fatty Acid Composition in Mediterranean Populations of the Marine Chlorophyte, Anadyomene stellata
Authors: Bemis Debra L. , Roussis Vassilios , Vagias Constantinios , Jacobs Robert S. 
Published: February 2015

Abstract
Chloroplasts isolated from three populations of the tropical marine Chlorophyte Anadyomene stellata collected off the coast of Greece were analyzed for their fatty acid composition. Following the preparation of fatty acid methyl esters, GC-MS (El) was utilized to identify the fatty acids present in each population. Including isomers, the fatty acid profile of all three algal populations was comprised of 19 fatty acids (4 saturated, 6 monounsaturated, 9 polyunsaturated) with palmitic acid present in the highest amounts (25-27% of total fatty acids). Analysis of variance revealed significant differences amongst the three populations in the percent of total fatty acids for twelve of the fatty acids. High levels of C20 PUFAs, an atypical observation in Chlorophytes, were observed in all three populations comprising approximately 17% of total fatty acids. Furthermore a 14:2 PUFA , apparently rare in marine macrophytic Chlorophytes, was identified in significant quantities. Surprisingly, we did not find any of the conjugated tetraene containing fatty acids that we previously identified in the A. stellata populations studied from the Florida Keys.
Purification of Tetragalloylglucose 4-O-Galloyltransferase and Preparation of Antibodies against This Key Enzyme in the Biosynthesis of Hydrolyzable Tannins
Authors: Grundhöfer Petra , Gross Georg G. 
Published: February 2015

Abstract
The enzyme, β-glucogallin: 1,2,3,6-tetra-O-galloyl- β-ᴅ-glucose 4-O-galloyltransferase, which catalyzes the last common step in the biosynthesis of the two subclasses of hydrolyzable tannins, i.e. gallotannins and ellagitannins, was purified 868-fold from leaves of pedunculate oak ( Quercus robur, syn. Q. pedunculata) to apparent homogeneity. Polyclonal antibodies against this pivotal enzyme were raised in rabbits and purified by protein-A chromatography, gel-filtration and affinity complexation. They were found to react specifically with acyltransferase from oak, displaying no cross-reactivity towards analogous enzymes from other plants synthesizing hydrolyzable tannins along the same biogenetic route, e.g. Rhus typhina or Tellima grandiflora.
Chemical Modification of the Mitochondrial Complex I Inhibitor 1-Trichloromethyl-1,2,3,4-tetrahydro-β-carboline: Synthesis and Evaluation of N-Alkanoyl Derivatives
Authors: Bringmann Gerhard , Feineis Doris , God Ralf , Brückner Ralph , Achim-Protzen Jens , Blank Michael , Peters Karl , Peters Eva-Maria , Janetzky Bernd , Reichmann Heinz 
Published: February 2015

Abstract
Several N-alkanoyl derivatives (4-9 and 13-16) of the potent mitochondrial complex I inhibitor TaClo (1-trichlorom ethyl-1,2,3,4-tetrahydro-β-carboline, 2) have been synthesized in order to elucidate the role of hydrophobic portion in the inhibitory action. Using rat brain homogenates or submitochondrial particles, the inhibitory effects of these compounds towards NADH-ubiquinone reductase (complex I) activity indeed appeared to correlate quite strongly with their lipophilic character. An X-ray structure analysis, exemplarily performed for N-acetyl-TaClo (4), revealed the N-substituent of such chlorinated agents to be dramatically pushed out of the β-carboline ring ‘plane’ due to the high steric demand of the huge trichloromethyl group at C-1.
Iron Release from the Active Site of Lipoxygenase
Authors: Fuchs Claus , Spiteller Gerhard 
Published: February 2015

Abstract
In the course of the lipoxygenase-catalyzed transformation of linoleic acid to 13S-hydroperoxy- 9Z,11E-octadecadienoic acid, iron ions are liberated. This iron release has been determined using a spectrophotometric assay based on the complexation of ferrous iron by 3-(2-pyridyl)-5,6-bis-(4-phenylsulfonic acid)-1,2,4-triazine disodium salt (ferrozine). Further comparative measurements demonstrated that iron release correlates to deficient oxygen supply. We speculate that release of iron ions is caused by modifications of histidine residues located at the active site of the enzyme. Liberation of iron ions may be responsible for increased generation of lipid peroxidation (LPO) products observed after a myocardial infarction since iron ions induce nonenzymic LPO processes.
Inhibition of Angiotensin - Converting Enzyme by a Synthetic Peptide Fragment of Glyceraldehyde-3-phosphate Dehydrogenase
Authors: Seweryn Ewa , Pędyczak Artur , Banaś Teresa 
Published: February 2015

Abstract
A novel inhibitor of angiotensin - converting enzyme (ACE) identical to a sequence part of human muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was chemically synthesized. Amino acid sequence was as follows Pro - Glu - Asn - Ile - Lys - Trp - Gly - Asp. This peptide inhibited rabbit lung ACE with a K1 value of 1.6 μм. The inhibitor of ACE acts in a non-competitive manner. The amino acid sequence of the new inhibitor was com- pared of GAPDH from other sources.
Two New Acylated Iridoid Glycosides from Verbascum undulatum
Authors: Magiatis Prokopios , Melliou Eleni , Tsitsa Eugenia , Charvala Catherine , Mitaku Sofia 
Published: February 2015

Abstract
On further phytochemical investigation of the aerial parts of Verbascum undulatum, two new acylated iridoid glycosides, 6-O-[3-O-(trans-3,4-dim ethoxycinnamoyl)-α- ʟ-rhamnopyranosyl] aucubin (1) and 6-O -[3-O-(trans-p-methoxycinnamoyl)-α-ʟ-rhamnopyranosyl]aucubin (2) were isolated. These structures were determined by spectral methods, mainly by 1D and 2D NMR spectroscopy.
Modified-release hydrocortisone decreases BMI and HbA1c in patients with primary and secondary adrenal insufficiency
Authors: Quinkler Marcus , Miodini Nilsen Roy , Zopf Kathrin , Ventz Manfred , Øksnes Marianne 
Published: February 2015

ObjectivePatients with adrenal insufficiency (AI) have impaired health-related quality of life (HRQoL), which is thought to be in part due to unphysiological glucocorticoid replacement therapy. The aim was to compare once-daily hydrocortisone (HC) dual-release tablet (modified-release) with conventional HC therapy regarding clinical data and HRQoL.Design and methodsWe conducted an open, prospective trial at one endocrine center. There were 15 of 26 patients with primary AI, nine of 18 patients with secondary AI, and six congenital adrenal hyperplasia patients switched to modified-release HC therapy by their own decision. We evaluated clinical outcome and disease-specific HRQoL by using AddiQoL questionnaire at baseline and at follow-up (median 202 days (85–498)).ResultsPatients on modified-release HC (n=30) showed significant decreases in BMI (26.0±0.75–25.6±0.71, P for change=0.006) and HbA1c (6.04±0.29–5.86±0.28, P for change=0.005), whereas patients remaining on conventional HC (n=20) showed no change in these parameters (P for interaction=0.029 and 0.017 respectively). No significant change in AddiQoL score were found in the modified-release HC group (83.8 baseline and 84.9 at follow-up; P for change=0.629). In the conventional HC group, there was a significant decrease in scores (84.0 baseline and 80.9 at follow-up; P for change=0.016), with a between-treatment P for interaction of 0.066. The fatigue subscore of AddiQoL showed the same pattern with a significant decrease (P for change=0.024) in patients on conventional HC therapy (P for interaction=0.116).ConclusionsModified-release HC decreases BMI and HbA1c compared with conventional HC treatment. In addition, it seems to stabilize HRQoL over time.
Sense of coherence modifies the effect of overtime work on mental health
Authors: Ohta Masanori , Higuchi Yoshiyuki , Yamato Hiroshi , Kumashiro Masaharu , Sugimura Hisamichi 
Published: February 2015

Sense of coherence modifies the effect of overtime work on mental health: Masanori Ohta, et al. Department of Health Development, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, JapanObjectiveIn the occupational health field, it is important to know how workload influences mental health. Overtime work and job strain appear to affect the mental health status of workers. Sense of coherence (SOC) may mediate the relationship between work stress and mental health. Since SOC represents a personal ability to manage psychological stressors, we hypothesized that a strong SOC would modify the adverse effect of an objective measure of overtime work on mental health.MethodsA total of 1,558 Japanese workers employed in an information technology company were asked to complete a 3‐item SOC Questionnaire and 28‐item General Health Questionnaire (GHQ) to assess mental health status. Workload was assessed by the actual amount of overtime work hours recorded by the company.ResultsMultiple regression analysis revealed a main effect of overtime work (β =0.08, p =0.0003) and SOC scores (β=0.41, p <0.0001) on GHQ scores. There was a tendency toward interaction between overtime work and SOC scores (β =0.05, p =0.051). Simple slope analysis supported this association (−1 SD below the mean, simple slope=0.04, SE=0.01, p <0.0001; +1 SD above the mean, simple slope=0.01, SE=0.01, p=0.188).ConclusionsThese results suggest that SOC buffers the mental health impacts of workload as measured by an objective index of overtime work, and should be considered when assessing the effects of workload on mental health.
AVALIAÇÃO PRELIMINAR DA QUALIDADE DO ESPERMATOZOIDE HUMANO APÓS EXPOSIÇÃO IN VITRO AO HERBICIDA 2,4-D
Authors: Garbiatti de Oliveira Tamiris , Ribas Pereira Viviane , Sanches Rodrigues Fernanda 
Published: February 2015

Nome (s) do Orientador (a) (s) Ana Paula Alves Favareto Patricia Alexandra Antunes Instituição ou Empresa UNIVERSIDADE DO OESTE PAULISTA - UNOESTE Instituição (s) de Fomento Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes) E-mail de contato tamigarbiatti@gmail.com Palavras-chave Agrotóxicos. Poluição. Espermatozoide humano.
SAÚDE E EDUCAÇÃO AMBIENTAL: UM RELATO DE CASO NO MUNICIPIO DE TEODORO SAMPAIO, SP.
Authors: Sarti Jaqueline Adelânia , Dias Leonice Seolin , Silva Junior Jovelino José da 
Published: February 2015

Este artigo apresenta o relato de experiência do trabalho desenvolvido entre universidade e poder público na área da saúde e da educação ambiental no município de Teodoro Sampaio. O objetivo do trabalho foi sensibilizar a comunidade escolar, em especial os alunos, acerca da importância dos cuidados com o meio ambiente para assegurar uma saúde pública mais saudável, evidenciando as correlações entre um (meio ambiente) e outro (saúde pública). Nesta parceria, a universidade, pautada por pesquisas cientificas, desenvolveu na Semana do Meio Ambiente um trabalho de sensibilização dos alunos acerca das implicações decorrentes das ações antrópicas no meio ambiente. O trabalho privilegiou as ações antrópicas que promovem a proliferação de agentes patogênicos, assim como as implicações dessa proliferação no que diz respeito à ocorrência de doenças no homem. No ambiente escolar foram desenvolvidas as atividades em conjunto com os educadores de forma que os alunos pudessem ser protagonistas do processo educativo por meio de atividades que lhes permitissem relacionar conhecimentos de disciplinas e áreas distintas (biologia e geografia; saúde e educação ambiental), se situarem no seu contexto sócio-espacial e se expressarem artisticamente. Por fim avaliamos positivamente tal ação de sensibilização, a qual, integradas com outras, contribui com o processo de conscientização ambiental e mudança de atitudes.
OCORRÊNCIA DE HORMÔNIOS ESTROGÊNICOS NO AMBIENTE
Authors: Tenália Ana Beatriz Marchini , Matsumoto Tsunao , Manoel Letícia de Oliveira 
Published: February 2015

Nome (s) do Orientador (a) (s) Prof. Dr. Tsunao Matsumoto Instituição ou Empresa Universidade Estadual Paulista “Júlio de Mesquita Filho”- UNESP/FEIS Instituição (s) de Fomento E-mail de contato anabeatriz_mt@yahoo.com.br Palavras-chave Disruptores endócrinos, Estrógenos. Efluentes.
AVALIAÇÃO DOS POTENCIAIS TOXICOLÓGICOS DA MISTURA ENTRE OS SOLVENTES METANOL E ACETONITRILA EM DAPHINIA SIMILIS E DAPHINIA MAGNA
Authors: Penatti Fábio Eduardo , Sesso Josélia , Fusato Felipe 
Published: February 2015

Nome do orientador: Valdemar Luis Tornisielo Instituição Universidade de São Paulo – Programa de Pós-Graduação Interunidades em Ecologia Aplicada E-mail do contato: f.penatti@usp.br Palavras-chave Solventes orgânicos; Toxidade; Microcrustáceos
OCORRÊNCIA DE DÍPTEROS MUSCÓIDES (FANNIDAE) NO MUNICÍPIO DE TEODORO SAMPAIO, SÃO PAULO
Authors: Theotonio Giovanni Enrico , Dias Leonice Seolin 
Published: February 2015

Nome (s) do Orientador (a) (s) Instituição ou Empresa Universidade Estadual Paulista (UNESP) de Presidente Prudente, SP Instituição (s) de Fomento Biota/FAPESP E-mail de contato giovannienrico@live.com Palavras-chave Fanniidae. Bioindicadores. Morro do Diabo
BIODIVERSIDADE E CONHECIMENTOS TRADICIONAIS: PRESERVAÇÃO OU APROVEITAMENTO?
Authors: Nassaro Adilson Luís Franco 
Published: February 2015

Biodiversidade e conhecimentos tradicionais são temas apresentados em conjunto quando relacionados à exploração das possibilidades dos recursos naturais, em avançado nível que as pesquisas científicas têm viabilizado, e também às preciosas informações sobre o manuseio desses mesmos recursos pelas comunidades tradicionais. Acompanham o dueto candentes questões quanto aos titulares dos benefícios supervenientes. Existem duas frentes no Brasil contemporâneo que defendem, respectivamente, as hipóteses de preservação e de aproveitamento do patrimônio genético no país; essa dualidade reflete um antagonismo em função da burocracia imposta por legislação restritiva à bioprospecção que busca impedir indevidas apropriações em defesa do potencial desenvolvimento do país, ao mesmo tempo em que também inibe o avanço das pesquisas de iniciativa nacional. Enquanto não se resolve o impasse estabelecido, outros fatores de impacto sobre o meio natural provocam extinção de espécies antes do seu idealizado aproveitamento. Palavras-chave: Biodiversidade. Conhecimentos tradicionais. Bioprospecção.
Real-Time Process Assurance of Offshore Cementing Operations
Authors: Jose Sunil Jacob , Daulton Daniel J 
Published: February 2015

Objectives/Scope:
In the high risk, highly technical and demanding environment of offshore oil and gas operations there are very few items more critical to the safe and effective execution of the overall program than well control and isolation. Regarding well isolation, multiple barriers require the cementing job plan and the corresponding procedures be conducted as proposed. In addition, the cement unit and supporting equipment need to be available and reliable during the job. Both are keys to the overall success of the cementing operation. With the realities of offshore cementing in mind, the industry wide challenge in finding appropriate talent in the respective regions further complicates the cementing service delivery. This type of situation requires a step change in the way cementing operations are currently done to drive the necessary process and quality assurance. Taking lessons learned from other industries with similar risk profiles, process and quality assurance can be achieved by providing: • A “second set of eyes” to drive the necessary process and quality assurance • Access to a trusted advisor that will back up remote resources during the critical junctures of the job By leveraging the latest in situational awareness and real-time technologies, offshore cementing will usher in a new age where the cementing specialist can access the knowledge and live assistance of experts across the world. This paper will discuss the approach and benefits of enabling real-time remote advisory services for remote cementing operations. Methods, Procedures, Process:Approach: • Provide real-time video and audio surveillance and collaboration to remote experts • Configure proposed cementing job procedures and synchronize it to relevant video feeds to enable remote expert response and process validation as required • Record and report against relevant data, real-time and post job analysis Results, Observations, Conclusions:Benefits: • Process Assurance - Validation and verification that key steps are being performed as planned • Operational Efficiency - Remote experts are available on-demand regardless of geographical location • Traceability - Record of operational activities to support compliance, knowledge transfer and process improvement • Reduced Project NPT - With technology leveraged surveillance to improve quality assurance Novel/Additive Information: n/a