bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of Soluble 14‐3‐3∊ as a Novel Subchondral Bone Mediator Involved in Cartilage Degradation in Osteoarthritis
Authors: Priam Sabrina , Bougault Carole , Houard Xavier , Gosset Marjolaine , Salvat Colette , Berenbaum Francis , Jacques Claire 
Published: April 2013

ObjectiveMechanical stress plays an important role in cartilage degradation and subchondral bone remodeling in osteoarthritis (OA). The remodeling of the subchondral bone could initiate cartilage loss in OA through the interplay of bone and cartilage. The aim of this study was to identify soluble mediators released by loaded osteoblasts/osteocytes that could induce the release of catabolic factors by chondrocytes.MethodsMurine osteoblasts/osteocytes were subjected to cyclic compression, and then conditioned medium from either compressed (CCM) or uncompressed (UCM) cells was used to stimulate mouse chondrocytes. Chondrocyte expression of matrix metalloproteinase 3 (MMP‐3), MMP‐13, type II collagen, and aggrecan was assessed by reverse transcription–polymerase chain reaction, Western blotting, and enzyme‐linked immunosorbent assay. Soluble mediators released by compressed osteoblasts/osteocytes were identified using iTRAQ (isobaric tags for relative and absolute quantification), a differential secretome analysis. Subchondral bone and cartilage samples were isolated from OA patients, and culture medium conditioned with OA subchondral bone or cartilage was used to stimulate human chondrocytes.ResultsStimulation of mouse chondrocytes with CCM strongly induced the messenger RNA (mRNA) expression and protein release of MMP‐3 and MMP‐13 and inhibited the mRNA expression of type II collagen and aggrecan. Differential secretome analysis revealed that 10 proteins were up‐regulated in compressed osteoblasts/osteocytes. Among them, soluble 14‐3‐3∊ (s14‐3‐3∊) dose‐dependently induced the release of catabolic factors by chondrocytes, mimicking the effects of cell compression. Addition of a 14‐3‐3∊ blocking antibody greatly attenuated the CCM‐mediated induction of MMP‐3 and MMP‐13 expression. Furthermore, in human OA subchondral bone, s14‐3‐3∊ was strongly released, and in cultures of human OA chondrocytes, s14‐3‐3∊ stimulated MMP‐3 expression.ConclusionThe results of this study identify s14‐3‐3∊ as a novel soluble mediator critical in the communication between subchondral bone and cartilage in OA. Thus, s14‐3‐3∊ may be a potential target for future therapeutic or prognostic applications in OA.
Overexpression of Sirtuin 6 Suppresses Inflammatory Responses and Bone Destruction in Mice With Collagen‐Induced Arthritis
Authors: Lee Hwa‐Suk , Ka Sun‐O , Lee Sang‐Myeong , Lee Sang‐Il , Park Jin‐Woo , Park Byung‐Hyun 
Published: April 2013

ObjectiveSirtuin 6 (SIRT‐6) is an NAD+‐dependent deacetylase and mono‐ADP‐ribosyltransferase. It is known to interfere with the NF‐κB signaling pathway and thereby has an antiinflammatory function. Due to the central role of NF‐κB in rheumatoid arthritis (RA) development, we undertook this study to test our hypothesis that SIRT‐6 could have antiarthritic effects.MethodsAn adenovirus containing SIRT‐6 complementary DNA (Ad‐SIRT6) was used to deliver SIRT‐6 to human RA fibroblast‐like synoviocytes in vitro as well as to mice with collagen‐induced arthritis (CIA) in vivo via bilateral intraarticular injections into the ankle joints.ResultsIn vitro experiments demonstrated that SIRT‐6 overexpression suppressed NF‐κB target gene expression induced by tumor necrosis factor α. SIRT‐6 overexpression inhibited osteoclast differentiation induced by macrophage colony‐stimulating factor and RANKL in bone marrow–derived macrophages. Mice with CIA had an increased incidence of disease and developed arthritis in the hind paws. In contrast, mice injected with Ad‐SIRT6 showed attenuated severity of arthritis based on clinical scores, hind paw thickness, and radiographic and pathologic findings. Moreover, the injection of Ad‐SIRT6 down‐regulated local and systemic levels of proinflammatory cytokines. After induction of CIA, mice injected with Ad‐SIRT6 showed significantly decreased arthritis severity, from the onset of clinical signs to the end of the study.ConclusionThese results suggest that blocking the NF‐κB pathway by SIRT‐6 in rheumatoid joints reduces both the inflammatory response and tissue destruction. Therefore, the development of an immunoregulatory strategy based on SIRT‐6 may have therapeutic potential for the treatment of RA.
A Less Conservative Stability Condition of Time‐Delay Systems with Polytopic Uncertainty
Authors: Wu Ai‐Guo , Lin Qingqiang , Fu Yan‐Ming , Duan Guang‐Ren 
Published: April 2013

AbstractThis paper considers the problem of stability analysis of time‐delay systems with time‐varying delay. By introducing a small scalar, the products between system matrices and Lyapunov matrices are decoupled with the help of the introduced additional matrices. Therefore, when the time‐delay systems with polytopic uncertainties are investigated, the vertex‐dependent Lyapunov function can be allowed, and thus the conservativeness can be reduced. Numerical examples are given to verify the effectiveness of the proposed results.
Long‐term outcomes of complete versus incomplete revascularization after drug‐eluting stent implantation in patients with multivessel coronary disease
Authors: Gao Zhan , Xu Bo , Yang Yue‐jin , Yuan Jin‐qing , Chen Jue , Chen Ji‐lin , Qiao Shu‐bin , Wu Yong‐jian , Yan Hong‐bin , Gao Run‐lin 
Published: April 2013

BackgroundA limited number of studies on the impact of complete revascularization (CR) vs. incomplete revascularization (IR) on long‐term outcomes in patients with multivessel coronary disease (MVD) in current percutaneous coronary intervention (PCI) practice have yielded inconsistent results.MethodsBetween April 2004 and November 2010, 7,376 consecutive patients with MVD underwent PCI at the Fuwai Hospital in Beijing, China. Patients who underwent prior CABG and those who had an acute myocardial infarction (MI) within 24 hr before revascularization or presented with cardiogenic shock were excluded. Angiographic CR was defined as successful angioplasty of all diseased lesions in the major epicardial coronary vessels and their first degree side branches (diameter ≥2.5 mm), and proximal CR was defined as successful angioplasty of all diseased proximal arteries.ResultsAmong 7,065 patients with MVD undergoing PCI treatment, angiographic CR was performed in 1,188 patients (16.8%), and proximal CR in 2,053 patients (29.1%). The study found that either angiographic or proximal IR were associated with significantly higher estimated 3‐year rate of cardiac death (2.55% vs. 1.13%, log‐rank P = 0.016; and 2.70% vs. 1.43%, log‐rank P = 0.024, respectively). After adjustment for differences in baseline characteristics between IR and CR patients, angiographic IR was associated with a significantly higher rate of cardiac death (adjusted hazards ratio [HR]: 2.56, 95% confidence interval [CI]: 1.03–6.41) while proximal IR was associated with a numerically higher rate of cardiac death (adjusted HR: 1.72, 95% CI: 0.93–3.17). For the subgroup of ≥2‐vessel IR with total occlusion, either angiographic or proximal IR patients had significantly higher rate of cardiac death (adjusted HR: 4.25, 95% CI: 1.50–12.09; and adjusted HR: 3.02, 95% CI: 1.40–6.52, respectively).ConclusionCompared with IR, patients with CR had better clinical outcomes, supporting CR as first choice for patients with MVD. © 2013 Wiley Periodicals, Inc.
Transcatheter closure of perimembranous ventricular septal defect using amplatzer ductal occluder
Authors: Lee Sang Mi , Song Jin Young , Choi Jae Young , Lee Sang Yoon , Paik Jae Sook , Chang So Ick , Shim Woo Seop , Kim Seong Ho 
Published: April 2013

ObjectiveTo show that transcatheter closure of perimembranous ventricular septal defect (PMVSD) with the Amplatzer ductal occluder (ADO, AGA Medical Corp, Plymouth, Minnesota) is a safe and effective treatment option.BackgroundTranscatheter closure of PMVSD is a challenging procedure. Recently, the Amplatzer PMVSD occluder (APMVSDO, AGA Medical Corp, Plymouth, Minnesota) has been shown to be effective in closing hemodynamically significant PMVSDs. However, the high incidence of complete atrioventricular block (CAVB) after device occlusion of a PMVSD has been a hot issue as well.MethodsAmong several devices used in closing PMVSD percutaneously, we prefer the ADO because of the anatomic resemblance between PMVSD with aneurysm and patent ductus arteriosus, and it has no right ventricular disc which may contribute to the occurrence of CAVB.ResultsBetween August 2009 and May 2012, 21 patients (5 males and 16 females) underwent percutaneous PMVSD closure using ADO. The patients' ages ranged from 3 to 42 years (median: 7 years), and their weights ranged from 18 kg to 60 kg (median: 27 kg). All patients showed echocardiographic signs of left ventricular volume overload and trivial to small mitral regurgitation (Qp/Qs = 1.7 ± 0.4). The mean defect size of the right ventricular side was 4.3 ± 1.0 mm. Devices 2 mm larger than the measured narrowest VSD diameter were selected in most patients. The ADOs were successfully implanted in all patients without any significant complications except one transient CAVB, one case of delivery wire fracture, and one case of surgery due to significant residual leak. Small residual shunts were observed immediately after the device implantation, but they disappeared during follow‐up for 18 of 20 patients. The mean follow‐up period was 20 ± 9 months, and CAVB or aortic regurgitation was not observed in all patients.ConclusionTranscatheter closure of PMVSD with the ADO is a safe and promising treatment option, but long‐term follow‐up in a large number of patients would be warranted. © 2013 Wiley Periodicals, Inc.
Differences in recovery of left and right ventricular function following aortic valve interventions: A longitudinal echocardiographic study in patients undergoing surgical, transapical or transfemoral aortic valve implantation
Authors: Forsberg Lena M. , Tamás Éva , Vánky Farkas , Engvall Jan , Nylander Eva 
Published: April 2013

ObjectivesTo evaluate longitudinal left and right ventricular function (LVF and RVF) after transcatheter aortic valve implantation (TAVI) as compared to surgical aortic valve replacement (SAVR) and LVF and RVF after TAVI by the transfemoral (TF) or transapical (TA) approach.BackgroundKnowledge about differences in recovery of LVF and RVF after TAVI and SAVR is scarce.MethodsSixty patients (age 81 ± 7 years, logistic EuroSCORE 16 ± 10%), undergoing TAVI (TF: n = 35 and TA: n = 25), were examined by echocardiography including atrioventricular plane displacement (AVPD) and peak systolic velocities (PSV) by tissue Doppler at basal RV free wall, LV lateral wall and septum preprocedurally, 7 weeks and 6 months postprocedurally. Twenty‐seven SAVR patients were matched to 27 TAVI patients by age, gender and LVF.ResultsEarly postintervention, TAVI patients had improved longitudinal LVF. However, when analyzed separately, only TF, but not TA patients, had improved LV lateral and septal AVPD and PSV (all P ≤ 0.01). All TAVI patients, as well as the TF and TA group had unchanged longitudinal LVF between the early and late follow‐ups (all P > 0.05). The SAVR group had higher septal LVF than the matched TAVI group preprocedurally, while postoperatively this difference was diminished. Longitudinal RVF was better in the TF group than in the TA group pre‐ and postprocedurally. Although the SAVR group had superior longitudinal RVF preoperatively, this was inferior to TAVI postoperatively.ConclusionsPostprocedural longitudinal LVF and RVF in patients undergoing TF‐TAVI, TA‐TAVI, or SAVR differ considerably. Preservation of longitudinal RVF after TAVI might influence the selection of aortic valve intervention in the future.© 2013 Wiley Periodicals, Inc.
Driving Corporate Social Responsibility in the Malawian Mining Industry: A Stakeholder Perspective
Authors: Mzembe Andrew Ngawenja , Meaton Julia 
Published: April 2013

ABSTRACTWhile there has been a growing body of research focused on corporate social responsibility (CSR) practices in developing economies, few studies have examined the factors shaping the CSR agenda in sub‐Saharan countries. Using qualitative data obtained through semi‐structured interviews with management and stakeholders, this paper examines the drivers of the CSR agenda pursued by Paladin (Africa), a subsidiary of an Australian multinational mining company (MNC) operating the first uranium mine in Malawi. The findings suggest that the CSR agenda in the mining industry in Malawi is strongly influenced by externally generated pressures such as civil society organisation activism and community expectations; although it is clear that other drivers such as public and private regulations and pressure from financial markets also played a role in pressurising Paladin to adopt a CSR agenda. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment
Weakness of remote authentication scheme of Chen et al.
Authors: Tang Hong‐bin , Liu Xin‐song , Chen Jian‐ying 
Published: April 2013

SUMMARYThe extensive application of mobile commerce has led researchers to design more secure protocols for mobile devices during the recent years. In 2011, Chen et al. proposed a three‐factor mobile device‐based remote authentication scheme, which tackled the security risk imposed by the loss of both password and mobile device. Scheme of Chen et al., however, is still vulnerable to the privileged insider attack, the replay attack, the impersonation attack, and the denial of service attack. It is not feasible for real‐life implementation. Copyright © 2013 John Wiley & Sons, Ltd.
An improved t‐out‐of‐n e‐lottery protocol
Authors: Liu Y. , Lin D. , Cheng C. , Chen H. , Jiang T. 
Published: April 2013

SUMMARYIn 2009, Lee and Chang proposed an electronic t‐out‐of‐n lottery protocol over the Internet based on the Chinese remainder theorem and blind signature. However, a security flaw exists in Lee–Chang's protocol that the lottery agent and the malicious purchaser can collude to control the winning result, which is unfair for the honest participants. On the basis of a verifiable random number generated by using the Lagrange interpolation formula over a finite field, an improved t‐out‐of‐n e‐lottery protocol is proposed, which guarantees that each participant can generate the winning result equally. The improved protocol achieves public verifiability and fairness without a trusted third party and a delaying function, which makes it more robust and more efficient. Copyright © 2013 John Wiley & Sons, Ltd.
Power increases performance in a social evaluation situation as a result of decreased stress responses
Authors: Schmid Petra C. , Schmid Mast Marianne 
Published: April 2013

AbstractWe tested whether power reduces responses related to social stress and thus increases performance evaluation in social evaluation situations. We hypothesized and found that thinking about having power reduced fear of negative evaluation and physiological arousal during a self‐presentation task (Studies 1 and 2). In Study 2, we also showed that simply thinking about having power made individuals perform better in a social evaluation situation. Our results confirmed our hypotheses that the mechanism explaining this power–performance link was that high power participants felt less fear of negative evaluation. The reduced fear of negative evaluation generated fewer signs of behavioral nervousness, which caused their performance to be evaluated more positively (serial mediation). Simply thinking of having power can therefore have important positive consequences for a person in an evaluation situation in terms of how he or she feels and how he or she is evaluated. Copyright © 2013 John Wiley & Sons, Ltd.
Subsurface architecture of the Boulder Creek Critical Zone Observatory from electrical resistivity tomography
Authors: Leopold Matthias , Völkel Jörg , Huber Juliane , Dethier David 
Published: March 2013

ABSTRACTThe architecture of the critical zone includes the distribution, thickness, and contacts of various types of slope deposits and weathering products such as saprolite and weathered bedrock resting on solid bedrock. A quantitative analysis of architecture is necessary for many model‐driven approaches used by pedologic, geomorphic, hydrologic or biologic studies. We have used electrical resistivity tomography, a well‐established geophysical technique causing minimum surficial disturbance, to portray the subsurface electrical resistivity differences at three study sites (Green Lakes Valley; Gordon Gulch; Betasso) at the Boulder Creek Critical Zone Observatory (BcCZO). Possible limitations of the technique are discussed. Interpretation of the specific resistivity values using natural outcrops, pits, roadcuts and drilling data as ground truth information allows us to image the critical zone architecture of each site. Green Lakes Valley (3700 MASL), a glacially eroded alpine basin, shows a rather simple, split configuration with coarse blockfields and sediments, partly containing permafrost above bedrock. The critical zone in Gordon Gulch (2650 MASL), a montane basin with rolling hills, and Betasso (1925 MASL), a lower montane basin with v‐shaped valleys, is more variable due to a complex Quaternary geomorphic history. Boundaries between overlying stratified slope deposits and saprolite were identified at mean depths of 3.0 ± 2.2 m and 4.1 ± 3.6 m in the respective sites. The boundary between saprolite and weathered bedrock is deeper in Betasso at 5.8 ± 3.7 m, compared with 4.3 ± 3.0 m in Gordon Gulch. In general, the data are consistent with results from seismic studies, but electrical resistivity tomography documents a 0.5–1.5 m shallower critical zone above the weathered bedrock on average. Additionally, we document high lateral variability, which results from the weathering and sedimentation history and seems to be a consistent aspect of critical zone architecture within the BcCZO. Copyright © 2013 John Wiley & Sons, Ltd.
A Method to Detect Differentially Methylated Loci With Next‐Generation Sequencing
Authors: Xu Hongyan , Podolsky Robert H. , Ryu Duchwan , Wang Xiaoling , Su Shaoyong , Shi Huidong , George Varghese 
Published: April 2013

ABSTRACTEpigenetic changes, especially DNA methylation at CpG loci have important implications in cancer and other complex diseases. With the development of next‐generation sequencing (NGS), it is feasible to generate data to interrogate the difference in methylation status for genome‐wide loci using case‐control design. However, a proper and efficient statistical test is lacking. There are several challenges. First, unlike methylation experiments using microarrays, where there is one measure of methylation for one individual at a particular CpG site, here we have the counts of methylation allele and unmethylation allele for each individual. Second, due to the nature of sample preparation, the measured methylation reflects the methylation status of a mixture of cells involved in sample preparation. Therefore, the underlying distribution of the measured methylation level is unknown, and a robust test is more desirable than parametric approach. Third, currently NGS measures methylation at over 2 million CpG sites. Any statistical tests have to be computationally efficient in order to be applied to the NGS data. Taking these challenges into account, we propose a test for differential methylation based on clustered data analysis by modeling the methylation counts. We performed simulations to show that it is robust under several distributions for the measured methylation levels. It has good power and is computationally efficient. Finally, we apply the test to our NGS data on chronic lymphocytic leukemia. The results indicate that it is a promising and practical test.
Intensities of the Martian N2 electron‐impact excited dayglow emissions
Authors: Fox Jane L. , F. Hać Nicholas E. 
Published: April 2013

AbstractThe first N2 emissions in the Martian dayglow were detected by the SPICAM UV spectrograph on board the Mars Express spacecraft. Intensities of the (0,5) and (0,6) Vegard‐Kaplan bands were found to be about one third of those predicted more than 35 years ago. The Vegard‐Kaplan band system arises from the transition from the lowest N2 triplet state () to the electronic ground state (). It is excited in the Martian dayglow by direct electron‐impact excitation of the ground N2(X) state to the A state and by excitation to higher triplet states that populate the A state by cascading. Using revised data, we compute here updated intensities of several of the bands in the N2 triplet systems and those involving the a1Πg state, the upper state of the Lyman‐Birge‐Hopfield bands. We find that the predicted limb intensities for the (0,5) and (0,6) Vegard‐Kaplan bands are consistent with the measured values.
Transoral robotic surgery for the management of oropharyngeal minor salivary gland tumors
Authors: Villanueva Nathaniel L. , de Almeida John R. , Sikora Andrew G. , Miles Brett A. , Genden Eric M. 
Published: April 2013

BackgroundIn this study we report our preliminary experience with the use of transoral robotic surgery (TORS) for the management of minor salivary gland malignancies of the oropharynx and outcomes.MethodsThis was a retrospective review of 10 patients followed over a 5‐year period at a single tertiary academic medical center. Patient, tumor, clinicopathologic, functional, and oncologic outcomes were collected and analyzed.ResultsAll cancers treated were either T1 (40%) or T2 (60%). Negative margins were achieved in all patients. Four patients received adjuvant radiation therapy (40%). No patients experienced surgical complications and all had excellent functional outcomes. Mean follow‐up was 24 months (range, 2–60 months) with locoregional and distant control achieved in 8 patients (80%) and 9 patients (90%), respectively.ConclusionsMinor salivary malignancies of the oropharynx are a surgical disease. Short‐term follow‐up data demonstrate feasibility, low morbidity, good functional, and oncologic results for TORS management of these malignancies. © 2013 Wiley Periodicals, Inc. Head Neck 36: 28–33, 2014
USING MODIFIED VISUAL‐INSPECTION CRITERIA TO INTERPRET FUNCTIONAL ANALYSIS OUTCOMES
Authors: Roane Henry S. , Fisher Wayne W. , Kelley Michael E. , Mevers Joanna L. , Bouxsein Kelly J. 
Published: April 2013

AbstractThe development of functional analysis (FA) methodologies allows the identification of the reinforcers that maintain problem behavior and improved intervention efficacy in the form of function‐based treatments. Despite the profound impact of FA on clinical practice and research, questions still remain about the methods by which clinicians and researchers interpret FA graphs. In the current study, 141 FA data sets were evaluated using the structured visual‐inspection criteria developed by Hagopian et al. (1997). However, the criteria were modified for FAs of varying lengths. Interobserver agreement assessments revealed high agreement coefficients across expert judges, postdoctoral reviewers, master's‐level reviewers, and postbaccalaureate reviewers. Once the validity of the modified visual‐inspection procedures was established, the utility of those procedures was examined by using them to categorize the maintaining reinforcement contingency related to problem behavior for all 141 data sets and for the 101 participants who contributed to the 141 data sets.