bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correlation between Systemic Arterial Hypertension and Bone Morphogenetic Protein-2 in Central Obese Non-Diabetic Men with Evidence of Coronary Artery Calcification
Authors: Lukito Antonia Anna , Widyasanto Allen , Yulianti Trilis , Muljadi Rusli , Wijaya Andi , Kabo Peter , Bakri Syakib 
Published: October 2015

BACKGROUND: Previous studies have confirmed separately the relationship between obesity, insulin-resistance, hypertension and bone morphogenetic protein-2 (BMP-2) with coronary artery calcification, a parameter of subclinical atherosclerosis. It was also reported that BMPs may function as proinflammatory, prohypertensive and proatherogenic mediators. The study aimed to assess the correlation between systemic hypertension and BMP-2 plasma concentration in central-obese non-diabetic men with evidence of coronary artery calcification.METHODS: This was a cross sectional study on 60 central-obese non-diabetic men, of an average age of 55.2 years, with evidence of coronary calcification, who came for health check-up and met the inclusion criteria consecutively as defined by waist circumference >90 cm and fasting blood glucose <126 mg/dL. Coronary calcification was defined by coronary artery calcium (CAC) score ≥10 Agatson-unit Dual Source 64 slice CT scan.RESULTS: There is positive correlation between hypertension and BMP-2 in central-obese non-diabetic men with evidence of coronary artery calcification. BMP-2 plasma concentration was higher in the hypertensive subjects. The correlation was stronger in younger (<55 years old) subjects and subjects with insulin-resitance.KEYWORDS: hypertension, BMP-2, coronary calcification, central obesity, age, insulin resistance
Novel Sources of Fetal Stem Cells for Future Regenerative Medicine
Authors: Lina Yani , Wijaya Andi 
Published: October 2015

BACKGROUND: Mesenchymal stromal cells are multipotent cells considered to be of great promise for use in regenerative medicine. However, the cell dose may be a critical factor in many clinical conditions and the yield resulting from the ex vivo expansion of mesenchymal stromal cells derived from bone marrow may be insufficient. Thus, alternative sources of mesenchymal stromal cells need to be explored.CONTENT: There are multiple extra-embryonic tissues emerging during gestation including umbilical cord blood (UCB), amniotic fluid (AF), Wharton’s jelly, the amniotic membrane and the placenta, which are all discarded following birth. Fetal stem cells from these sources actually represent a new class of stem cells developmentally and operationally located between the state of embryonic stem cells and adult stem cells, sharing and exhibiting features of pluripotency and multipotency, without necessarily implying that they can generate every type of tissue.SUMMARY: Fetal stem cells have been recently isolated from several tissues (amniotic fluid, umbilical cord, Wharton’s jelly, amnion and placenta). They are derived either from the fetus proper or from the supportive extra-embryonic structures. They represent ideal sources for regenerative medicine since they are easily accessible, exhibit high proliferation rates, do not form teratomas and present no ethical reservations like embryonic stem cells (ESC). Their functional features indicate that they actually represent intermediates between ESC and adult stem cells.KEYWORDS: mesenchymal stem cells, fetal stem cells, amniotic fluid, umbilical cord, placenta, wharton’s jelly
Evaluation of Enhanced Condensate Recovery Potential in Shale Plays by Huff-n-Puff Gas Injection
Authors: Meng Xingbang , Sheng James J. , Yu Yang 
Published: October 2015

Abstract
In gas condensate reservoirs, production performance is compromised by the liquid dropout due to reservoir pressure depletion below the dew point pressure. In the shale gas-condensate plays, the condensate banking caused by a high pressure drop further reduce the productivity of the well. Evaluation of the condensate banking effect in shale reservoirs is critical to developing this type of shale condensate plays. Improving condensate recovery has a great economic potential to the oil industry. Condensate has a very good economic value because it contains heavier components in the reservoir. Also the reduction of condensate could increase the relative permeability of gas, the gas production could be improved. Published research shows that condensate banking problem is more significant for low permeability condensate systems. Therefore, this study to focus on this important issue: enhancing gas-condensate recovery in shale gas reservoirs.
In this paper, huff-n-puff gas injection in a shale gas reservoir is simulated to enhance gas-condensate recovery. The model used in the simulation work is calibrated with our enhanced condensate recovery experimental data which shows that huff-n-puff can effectively improve condensate recovery.
Based on the calibrated work, simulation study is performed to investigate the potential of huff-n-puff gas injection to enhance condensate recovery in shale plays. Different parameters are investigated in this simulation work: 1) different gas injection pressure, 2) different numbers of huff-n-puff cycles, 3) different soaking time.
The results of both the experimental and simulation work show that huff-n-puff method can effectively improve condensate recovery. During the huff period of cyclic gas injection, the pressure is increased, and the liquid dropout will be re-vaporized and then recovered during the puff period. We used 5 cycles of gas injection to improve condensate recovery and found that the first cycle recovered most of the condensate in the laboratory. The recovery efficiency of subsequent cycles was declined. The simulation work shows the similar recovery profile as observed in the laboratory.
Very limited research has been conducted to enhance condensate recovery in shale reservoirs. This paper focuses on examining the efficiency of huff-n-puff method to improve condensate recovery. Results indicate the applicability of huff-n-puff gas injection.
Impact of Geo-Mechanical Properties on the Fracture Treatment of Utica Shale
Authors: Osman Tarig M. , Bilgesu H. Ilkin 
Published: October 2015

Abstract
The main objective of this study was to understand the impact of formation geo-mechanical properties such as horizontal stress level, Young's modulus and Poison's ratio on the fracturing treatment outcome and also on the complex fracture growth. More precisely, the impact of these properties on the growth of discrete fracture network (DFN).
A single horizontal well model was built using commercially available software to simulate the fracturing treatment. The model was built based on Utica shale properties obtained from different sources. Different scenarios had been simulated based on the formation properties variation of Utica shale and also for different operational parameters such as treatment volume and injection rate. The simulation had been performed using the three dimension design concept to estimate the created fracture geometry and properties. The simulation also performed to compare between the single and multi-cluster fracturing treatment.
The results showed that the horizontal stress level plays a significant role in controlling the fracture orientation and growth and also affects the stimulated reservoir volume (SRV). The bi-wig fracture half length, width and volume were increased as the horizontal stress difference increased while the discrete fracture network (DFN) properties and the stimulated reservoir volume (SRV) were increased. The Young's modulus and the leak-off coefficient also impact the fracture and the discrete fracture network. The formations with high Young's modulus generated shorter and smaller bi-wing fracture and higher DFN length and stimulated reservoir volume (SRV). Thus, the hydraulic fracturing treatments in areas with high Young's modulus values will yield high SRV and better well performance. The increase of the leak-off coefficient will reduce the fracture and discrete fracture network volume.
This research can be extended to match the fracturing simulation results with the field micro-seismic data, and then use the matched results in production history matching for better understanding and evaluation of horizontal well performances in shale gas formations.
Water-Rock Interaction for Eagle Ford, Marcellus, Green River, and Barnett Shale Samples
Authors: Ali Maaz , Hascakir Berna 
Published: October 2015

Abstract
Knowledge of water-rock interactions on the surface of fractures is important to develop an understanding of the geological structures and changes within the formation, to determine the water pollutants in produced water during hydraulic fracturing, and to manage effectively the produced waters. To obtain this knowledge, the water-shale interaction is investigated on carbonate-rich (Eagle Ford), organic-rich (Green River), clay-rich (Barnett), and other minerals rich (Marcellus) shale samples.
Crushed shale samples were exposed to water for three weeks at reservoir conditions. The water and rock samples before and after each static experiment subjected to several analyses. The change in the rock mineralogy was defined with XRD, the elemental composition of rock was determined with XPS and SEM-EDS. The water was analyzed for its anions and cations, total dissolved solids (TDS), conductivity, pH, total organic carbon (TOC), and average particle sizes of colloids. The stability of the colloids are characterized with Zeta potential.
It has been observed that Barnett rock is high in illite content, the greatest calcite concentration is determined for Eagle Ford rock. The sulfate source of water correlates with the atomic percent of the sulfur and oxygen elements determined through XPS analyses. The magnesium content of water correlates mainly with illite amount in the rock and calcium concentration associates with the calcite and gypsum content of the rock samples. The greatest dissolution rate belongs to the minerals which yields sulfate in the water, then, gypsum and calcite which yield calcium cation in the water come second, and the lowest dissolution rates obtained from the magnesium containing minerals mainly dolomite. TDS of the water samples shows that Green River has the least tendency to interact with water and Barnett has the greatest tendency. Zeta potential values indicate that the particles in the water interacted with Eagle Ford have the highest tendency for precipitation, since it has the lowest zeta potential value.
This study provides correlations on water-rock interaction for four different shale samples. These correlations can be used to understand the water-rock interactions for different rocks by conducting simple XRD and XPS measurements only on rock samples.
Environmental Benefits of Electric Powered Hydraulic Fracturing
Authors: Oehring J. M. 
Published: October 2015

Abstract
The electric well stimulation fleet has shown great advances in environmental responsibility. The use of electric equipment reduces refueling traffic and has eliminated over 400 diesel deliveries to date. Emissions are reduced by over 99%. Fire risk is significantly reduced with some fire hazards being completely eliminated. The average sound pressure is reduced by 69% on the site. Electric powered hydraulic fracturing is an important step towards conducting well stimulation in a more environmentally responsible manner.
Traditional hydraulic fracturing fleets are powered by over twenty large diesel engines providing up to 2,500 BHP each. These environmental benefits are achieved by removing all diesel engines from a hydraulic fracturing site and replacing them with mobile turbine engine generator units. Then a fleet can be run completely on electric power. This technology was first deployed in 2014 in the Marcellus shale formation in West Virginia, and is the first fully electric, fully mobile well stimulation system completely fueled by natural gas.
The fleet generates electric power with three natural-gas-fueled turbine engine generators at 13,800 volts (V). The power generated by the turbines then runs through switchgear and is stepped down to 600 V using transformers. At this point, the electric fleet is similar to a conventional hydraulic fracturing fleet, except that all the fleet's diesel engines and transmissions are replaced by electric motors and variable frequency drives. Pumps, blenders, hydration, data van, and sand equipment are also all completely electric powered.
Approach for Assessing Wellbore Integrity to Prioritize Study Areas for Potential Siting of a Carbon Dioxide Repository
Authors: Buxton Bruce , Weber Stephanie , Moody Mark A. , Gupta Neeraj , Sminchak Joel 
Published: October 2015

Abstract
In this paper we present a proposed semi-quantitative approach for assessing the integrity of active and retired oil and gas wells in example study areas for siting geologic carbon storage repositories. The approach makes use of readily available wellbore information and is illustrated with study areas in Ohio and Michigan. Results help depict wellbore integrity issues in a region that has numerous oil and gas wells dating back to the 1800s. We first reviewed readily available sources of information and identified three key well condition indicators that might be expected to impact wellbore integrity – wellbore age, depth and status. Each of these indicators was then coded for each available well with a simple scale representing increasing levels of expected wellbore integrity and combined to form a composite integrity score. The resulting composite indicators provide a useful tool to help identify areas where existing wells tend to be newer, shallower, and plugged and abandoned– all of these being conditions more favorable for repository siting. When considered in combination with maps showing the depth of targeted geologic confining layers and maps indicating the density of existing wells (i.e., the number of existing wells per 1 km2 grid cell), the maps of wellbore integrity provide a more complete evaluation of different areas for repository siting. Study areas in Ohio and Michigan were evaluated and pros and cons of each area are discussed to help illustrate the utility of this proposed wellbore integrity approach. The approach proposed here makes use of reasonably extensive and readily available information, and combines it in a systematic and objective way, creating an intuitive tool to assist with repository siting.
Androgens, Bilateral Oophorectomy, and Cardiovascular Disease Mortality in Postmenopausal Women With and Without Diabetes: The Study of Osteoporotic Fractures
Authors: Appiah Duke , Winters Stephen J. , Muldoon Susan B. , Hornung Carlton A. , Cauley Jane A. 
Published: October 2015

OBJECTIVE
Diabetes elevates cardiovascular disease (CVD) risk more markedly in women than in men. Because the high risk of CVD among women with type 2 diabetes (DM2) may be partly due to increased ovarian androgen production, we investigated whether a history of bilateral salpingo oophorectomy (BSO) is inversely associated with CVD mortality among women with DM2.

RESEARCH DESIGN AND METHODS
Data were obtained from 7,977 women (a random subset of 564 had measurements of sex-steroid hormones) enrolled in the Study of Osteoporotic Fractures (SOF), a community-based, multicenter study that monitored women aged ≥65 years for a mean of 15.1 years. Adjusted hazard ratios (HRs) and 95% CIs were calculated using Cox proportional hazards regression.

RESULTS
The average age at baseline was 71.5 years, with 6.3% and 18% of participants reporting a history of diabetes or BSO, respectively. In the subset of the SOF cohort with sex-steroid hormone measurements, those with DM2 had 43.6% significantly higher levels of free testosterone that were partly explained by age and adiposity, whereas total and free testosterone levels were lower in women with BSO than in those with intact ovaries. CVD mortality was elevated in women with DM2 without BSO (HR 1.95, 95% CI 1.62–2.35) as well as in women with DM2 and BSO (HR 2.56, 95% CI 1.79–3.65; P = 0.190 for interaction). Overall, BSO was not associated with CVD mortality (HR 1.05, 95% CI 0.89–1.23).

CONCLUSIONS
The association of diabetes with CVD was not reduced by BSO, suggesting that ovarian hyperandrogenemia may not be a primary mechanism to explain the high risk for CVD among women with DM2.
Proinflammatory cytokine responses of cultured equine keratinocytes to bacterial pathogen‐associated molecular pattern motifs
Authors: LEISE B. S. , YIN C. , PETTIGREW A. , BELKNAP J. K. 
Published: October 2015

SummaryReasons for performing study: Further knowledge of equine keratinocyte physiology and keratinocyte response to various stimuli is important in developing a better understanding of disease states involving the epidermis.Objectives: To assess the inflammatory cytokine response of cultured equine keratinocytes to various pathogen‐associated molecular pattern molecules (PAMPs) from both Gram‐negative and positive bacteria likely to be present in equine sepsis.Methods: Keratinocytes were isolated from skin of 2 horses and primary cultures performed. Keratinocytes were harvested for RNA extraction after exposure to lipopolysaccharide (LPS), lipoteichoic acid (LTA), peptidoglycan (PGN), bacterial DNA (CpG), flagellin or maintained in medium (controls) for 4 or 24 h. Real time‐quantitative PCR was used to quantify interleukin‐1β (IL‐1β), interleukin‐6 (IL‐6) and CXCL8 mRNA concentrations.Results: Increases (P<0.05) in IL‐1β, IL‐6 and CXCL8 mRNA concentrations were induced by LPS exposure compared to controls. Increased mRNA concentrations of both IL‐6 and CXCL8 were also noted (vs. controls) upon exposure to flagellin. Overall, responses were greater at 4 h. No increases (P>0.05) in cytokine expression by keratinocytes were present after LTA, PGN or CpG exposure.Conclusions: Increased proinflammatory cytokine expression in response to LPS and flagellin indicate that equine keratinocytes have functional TLR4 and TLR5 receptor signalling. However, the lack of keratinocyte stimulation by PGN, LTA or CpG provides no evidence for functional TLR2, TLR9 or NOD receptor signalling. These results suggest that equine keratinocytes are more responsive to PAMPs usually associated with Gram‐negative sepsis and unresponsive to PAMPs most commonly associated with Gram‐positive sepsis.Potential relevance: The increased incidence of injury of epidermal structures in clinical cases of Gram‐negative (vs. Gram‐positive) sepsis in the horse may be due to a lack of functional TLR signalling for Gram‐positive PAMPs in the equine keratinocyte.
Biomechanics of a flexible sublaminar connector in long-segment thoracic fixation
Authors: Rodriguez-Martinez Nestor G. , Safavi-Abbasi Sam , Perez-Orribo Luis , Newcomb Anna G. U. S. , Reyes Phillip M. , Loughran Galyna , Theodore Nicholas , Crawford Neil R. 
Published: October 2015

OBJECT
The Universal Clamp Spinal Fixation System (UC) is a novel sublaminar connection for the spine that is currently used in conjunction with pedicle screws at the thoracic levels for the correction of scoliosis. This device allows the surgeon to attach rods and incorporate a pedicle screw construction. The flexible composition of the UC should provide flexibility intermediate to the uninstrumented spine and an all-screw construct. This hypothesis was tested in vitro using nondestructive flexibility testing of human cadaveric spine segments.

METHODS
Six unembalmed human cadaveric thoracic spine segments from T-3 to T-11 were used. The specimens were tested under the following conditions: 1) intact; 2) after bilateral screws were placed at T4-T10 and interconnected with longitudinal rods; 3) after placement of a hybrid construction with screws at T-4, T-7, and T-10 with an interconnecting rod on one side and screws at T-4 and T-10 with the UC at T5–9 on the contralateral side; (4) after bilateral screws were placed at T-4 and T-10 and interconnected with rods and bilateral UC were placed at T5–9; and 5) after bilateral screws at T-4 and T-10 were placed and interconnected with rods and bilateral sublaminar cables were placed at T5–9. Pure moments of 6.0 Nm were applied while optoelectronically recording 3D angular motion.

RESULTS
Bilateral UC placement and bilateral sublaminar cables both resulted in a significantly greater range of motion than bilateral pedicle screws during lateral bending and axial rotation, but not during flexion or extension. There were no differences in stability between bilateral UC and bilateral cables. The construct with limited screws on one side and UC contralaterally showed comparable stability to bilateral UC and bilateral cables.

CONCLUSIONS
These results support using the UC as a therapeutic option for spinal stabilization because it allows comparable stability to the sublaminar cables and provides flexibility intermediate to that of the uninstrumented spine and an all-screw construct. Equivalent stability of the hybrid, bilateral UC, and bilateral cable constructs indicates that 6-level UC provides stability comparable to that of a limited (3-point) pedicle screw-rod construct.
Isolation and characterization of microsatellite markers for Jasminum sambac (Oleaceae) using Illumina shotgun sequencing
Authors: Li Yong , Zhang Weirui 
Published: October 2015

Premise of the study:Microsatellite markers of Jasminum sambac (Oleaceae) were isolated to investigate wild germplasm resources and provide markers for breeding.Methods and Results:Illumina sequencing was used to isolate microsatellite markers from the transcriptome of J. sambac. A total of 1322 microsatellites were identified from 49,772 assembled unigenes. One hundred primer pairs were randomly selected to verify primer amplification efficiency. Out of these tested primer pairs, 31 were successfully amplified: 18 primer pairs yielded a single allele, seven exhibited fixed heterozygosity with two alleles, and only six displayed polymorphisms.Conclusions:This study obtained the first set of microsatellite markers for J. sambac, which will be helpful for the assessment of wild germplasm resources and the development of molecular marker–assisted breeding.
Manager : Un métier en mutation ? Essai de lecture rétro-prospective pour dessiner les contours du métier de manager et les accompagnements RH nécessaires
Authors: Payre Sébastien , Scouarnec Aline 
Published: October 2015

Les pratiques d’encadrement font l’objet de nombreuses critiques mais aussi de propositions pour mettre en œuvre de « nouveaux » modèles dans les entreprises. Une revue rétrospective de la littérature nous permettra de questionner les évolutions du métier de manager. Par la suite, nous mobiliserons une recherche-action réalisée pour la branche professionnelle de la communication publicitaire afin de dessiner les contours possibles du métier de manager demain et les nécessaires accompagnements RH qui en découlent.
La puissance fragile du corps : naissance et vulnérabilité
Authors: Pierron Jean-Philippe 
Published: October 2015

Comment parler du corps d’un bébé ? Répondre à une telle question nous révèle à la fois démunis et encombrés. Démunis parce qu’à chaque fois, à chaque naissance, une venue au monde est une survenue qui nous déroute et nous vulnérabilise. Lorsque vient au jour le petit humain, il mobilise dans sa fragilité toute l’énergie prometteuse d’un monde en ses puissances. Mais nous sommes tout autant encombrés et instruits par toute la culture qui parle ce corps, l’informe et l’investit. Elle cultive l’apparition du bébé pour l’installer dans un réseau de rituels, de concepts, de symbolisations qui apprivoisent, acclimatent son étrangeté. Plus particulièrement, dans notre culture, la méditation sur le corps du bébé a connu sa thématisation théologique et esthétique avec le thème de la Nativité.