bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel intralamellar semi‐bioresorbable keratoprosthesis—Part B: Surface functionalization and physico‐chemical characterization toward site‐specific cellular activity
Authors: Sunka Krishna Chaitanya , Byram Prasanna Kumar , Paikkattil Nidhin , Roy Trina , Chakravorty Nishant , Chaudhuri Bhaskar Ray , Dhara Santanu 
Published: August 2024

AbstractIn this article, site‐specific functionalized keratoprosthesis (KPro) was accomplished to meet epithelial on‐growth on the anterior optical surface, inhibit cell adhesion on the posterior optic surface, and promote cell ingrowth at the peripheral tissue‐haptic‐flange interface toward tissue integration. Gelatin immobilization on PC4 hydrogel surfaces was achieved through acid hydrolysis followed by EDC/NHS chemistry, and polyethylene glycol‐diacrylate (PEGDA)‐modified inter‐penetrating network (IPN) surfaces were prepared using photo‐polymerization of ethylene glycol‐diacrylate (EGDA) for enhancing and inhibiting cellular growth, respectively. The surface chemical structures of modified hydrogels were characterized using attenuated total reflectance‐infrared (ATR‐IR) spectroscopy and a bicinchoninic acid (BCA) protein adsorption assay. Further, suitable differentially functionalized surfaces were prepared and characterized using the universal testing machine (UTM), refractometer, atomic force microscopy (AFM), and contact angle measurements to understand mechanical, optical, surface morphological, and wettability properties, respectively. Finally, in vitro site‐specific cell adhesion, cell inhibition, and cell ingrowth functionalities were tested using isolated goat corneal limbal epithelial stem cells and corneal stromal fibroblast cells, respectively. Overall, the two connective articles represent a successful endeavor to develop a more esthetically pleasing, mechanically stable, tissue‐free hydrogel KPro with site‐specific surface functionality nearly mimicking the cornea's functionality. A further pre‐clinical investigation is necessary to advance this functional novel KPro to reality, especially the tissue integration aspect.
Comparison of the TNM9th and 8th editions for localized and locally advanced anal squamous cell carcinoma treated nonsurgically and proposal of a new stage grouping system
Authors: Zheng Aihong , Wang Yiwen , Li Shuang , Wang Yingjie , Xu Hong'en , Ding Jieni , Chen Bingchen , Song Tao , Lai Lei 
Published: August 2024

AbstractObjectiveTo compare the survival discrimination of the TNM9th and 8th editions for localized and locally advanced anal squamous cell carcinoma (ASCC) treated nonsurgically and suggest a simple revised staging system with data from the Surveillance, Epidemiology, and End Results (SEER) database.MethodsOverall survival (OS) was the primary endpoint. Survival comparisons between the T and N stages and the different staging systems were performed using the Kaplan–Meier method and log‐rank test, followed by correlation analysis and variable importance analysis (VIA). Additionally, multivariate analysis was employed to identify significant predictors, which were further visualized using a nomogram. Finally, calibration curve, C‐index, and decision curve analysis (DCA) were applied to assess the performance of the different staging systems.ResultsA total of 5384 patients with ASCC were analyzed, revealing superior discrimination OS by the TNM9th edition compared to that by the TNM8th edition. Multivariate analysis identified the T and N stages as significant OS predictors (all p < 0.001). However, ambiguity persisted in stage III subgroups within the TNM9th edition, showing OS times of 102 months for stage IIIA disease, 88 months for stage IIIB disease, and 128 months for stage IIIC disease (all p > 0.05). Correlation analysis demonstrated an increased correlation for the T stage between the TNM8th and 9th editions (ρ value from 0.7 to 0.89), while the N stage correlation decreased (ρ value from 0.84 to 0.56). VIA and the prognostic nomogram highlighted the greater importance of the T stage over the N stage. Based on these findings, a new staging system was developed, and its clinical utility was confirmed through calibration curves, C‐index values (from 0.598 to 0.604), and DCAs.ConclusionsOur new staging system exhibited slightly better prognostic value compared to the TNM9th staging systems for nonmetastatic ASCC and warrants further validation.
The role of DPYD and the effects of DPYD suppressor luteolin combined with 5‐FU in pancreatic cancer
Authors: Kato Hiroyuki , Sato Motonori , Naiki‐Ito Aya , Inaguma Shingo , Sano Makoto , Komura Masayuki , Nagayasu Yuko , Xiaochen Kuang , Kato Akihisa , Matsuo Yoichi , Ijichi Hideaki , Takahashi Satoru 
Published: August 2024

AbstractBackgroundDespite advances in the treatment of cancer, pancreatic ductal adenocarcinoma (PDAC) remains highly lethal due to the lack of effective therapies. Our previous study showed that Luteolin (Lut), a flavonoid, suppressed pancreatocarcinogenesis and reduced the expression of dihydropyrimidine dehydrogenase (DPYD), an enzyme that degrades pyrimidines such as 5‐fluorouracil (5‐FU), in PDACs. In this study, we investigated the role of DPYD and evaluated the therapeutic potential of combining 5‐FU with Lut in PDACs.Methods and ResultsPDAC cells overexpressing DPYD showed increased proliferation, and invasiveness, adding to the resistance to 5‐FU. The xenograft tumors of DPYD‐overexpressing PDAC cells also exhibit enhanced growth and invasion compared to the control xenograft tumors. RNA‐seq analysis of the DPYD‐overexpressing PDAC xenograft tumors revealed an upregulation of genes associated with metallopeptidase activity—MMP9 and MEP1A. Furthermore, the overexpression of MEP1A in PDAC was associated with invasion. Next, we investigated the combined effects of Lut, a DPYD suppressor, and 5‐FU on DPYD‐overexpressing xenograft tumors and PDAC of Pdx1‐Cre; LSL‐KrasG12D/+; Trp53flox/flox(KPPC) mice. Neither single administration of 5‐FU nor Lut showed significant inhibitory effects; however, the combined administration of 5‐FU and Lut exhibited a significant tumor‐suppressive effect in both the xenograft tumors and KPPC models.ConclusionWe have elucidated that DPYD expression contributes to proliferation, invasiveness, and 5‐FU resistance, in PDACs. The combination therapy of Lut and 5‐FU holds the potential for enhanced efficacy against PDACs.
The role of genetic variants in the prediction of hearing loss due to cisplatin chemoradiotherapy
Authors: Duinkerken Charlotte W. , Chiodo Sabrina , Hueniken Katrina , Hauptmann Michael , Jóźwiak Katarzyna , Cheng Dangxiao , Hope Andrew , Liu Geoffrey , Zuur Charlotte L. 
Published: August 2024

AbstractBackgroundConcomitant high‐dose cisplatin with radiotherapy is commonly used for treating head and neck squamous cell carcinoma (HNSCC). Cisplatin, often used with radiotherapy, is known for causing irreversible sensorineural hearing loss, with individual variability suggesting a genetic component. This study aims to enhance the predictive ability of the clinical prediction model for cisplatin‐induced hearing loss (CIHL) in HNSCC patients, as outlined in Theunissen et al., by incorporating significant genetic variants.MethodsConducted at the Netherlands Cancer Institute, this retrospective study included 74 patients treated between 1997 and 2011. Thirty‐one SNPs that were previously associated with CIHL or other cisplatin‐induced toxicities were identified and incorporated into the model. The primary outcome measured was the change in decibels at posttreatment 1‐2‐4 kHz hearing levels per additional minor allele of these SNPs, evaluated using linear mixed‐effects regression models. The model's predictive accuracy was determined by the area under the curve (AUC) using 10‐fold cross‐validation.ResultsThe rs2289669 SNP in the SLC47A1/MATE1 gene was linked to a significant 2.67 dB increase in hearing loss per allele (95% CI 0.49–4.86, p = 0.017). Incorporating rs2289669 improved the model's AUC from 0.78 to 0.83, a borderline significant improvement (p = 0.073).ConclusionsThis study underscores the importance of the rs2289669 SNP in CIHL and demonstrates the potential of combining genetic and clinical data for enhanced predictive models in personalized treatment strategies.
New Sulfonate Ester‐Linked Fluorinated Hydrazone Derivatives as Multitarget Carbonic Anhydrase and Cholinesterase Inhibitors: Design, Synthesis, Biological Evaluation, Molecular Docking and ADME Analysis
Authors: Akış Berna , Çakmak Reşit , Şentürk Murat 
Published: August 2024

AbstractIn this study, some new hydrazone derivatives (2a–g) was designed, synthesized for first time, and evaluated as multitarget inhibitors of AChE, BChE, hCA I and hCA II. The chemical structures of new hybrids were confirmed by elemental analysis and some spectroscopic techniques. All tested compounds showed low nanomolar inhibition with IC50 values of in the range of 30.4–264.0 nM against hCA I, 23.2–251.6 nM against hCA II, 12.1–114.3 nM against AChE, and 76.4–134.0 nM against BChE. These compounds inhibited hCA I and AChE more than acetazolamide (AZA) and neostigmine. Among them, compounds 2c and 2e, which have a linear structure, were determined to be the most active inhibitor candidates against these selected enzymes. Molecular docking studies were carried out on the compounds (2a‐‐g), revealing their binding interactions with the active site of AChE, BChE, hCA I and hCA II thus supporting the experimental findings. Additionally, in silico absorption, distribution, metabolism, and excretion (ADME) prediction studies of the obtained compounds (2a‐‐g) with in silico approaches were carried out to determine their solubility, whether they have the potential to cross the blood‐brain barrier (BBB), values such as GI absorption and drug likeness principles.
Expanding the Repertoire of ceDAF‐12 Ligands for Modulation of the Steroid Endocrine System in C. Elegans
Authors: Galilea Agustín , Santillán Vanessa J. , Acebedo Sofía L. , Virginia Dansey María , Álvarez Lautaro D. , Mazaira Gisela I. , Galigniana Mario D. , Castro Olga A. , Gola Gabriel F. , Ramírez Javier A. 
Published: August 2024

AbstractSteroid hormones are essential for the biological processes of eukaryotic organisms. The steroid endocrine system of C. elegans, which includes dafachronic acids (DA) and the nuclear receptor ceDAF‐12, provides a simple model for exploring the role of steroid hormone signaling pathways in animals. In this study, we show for the first time the feasibility of designing synthetic steroids that can modulate different physiological processes, such as development, reproduction and ageing, in relation to ceDAF‐12. Our results not only confirm the conclusions derived from genetic studies linking these processes but also provide new chemical tools to selectively manipulate them, as we found that different compounds produce different phenotypic results. The structures of these compounds are much more diverse than those of endogenous hormones and analogues previously described by other researchers, allowing further development of the chemical modulation of the steroid endocrine system in C. elegans and related nematodes.
Approaching a patient with poststernal pain after eating: A case report
Authors: Feng Yanjun , Niu Yayan , Yan Jinyu , Wu Meiying , Tang Peijun 
Published: August 2024

Key Clinical MessageThis article reports a case of mediastinal lymph node tuberculosis with no obvious symptoms and a concealed focus. This patient, a 33‐year‐old male, suffered from pain behind the sternum after eating. He underwent three gastroscopic examinations and two fine needle punctures guided by ultrasound gastroscopy but was not diagnosed. Chest‐enhanced CT revealed a mediastinal mass compressing the adjacent esophagus, suggesting the possibility of enlarged lymph nodes. Furthermore, T cells from patients infected with tuberculosis tested positive. Ultrasound bronchoscopy revealed enlarged lymph nodes in area 7, and then EBUS‐TBNA was performed in that region. Only a few scattered lymphocytes and necrotic tissue were found under the biopsy microscope. The EBUS‐TBNA biopsy Xpert MTB/RIF showed low positive results, and the EBUS‐TBNA puncture fluid Xpert MTB/RIF was positive. Therefore, he was diagnosed with mediastinal lymph node tuberculosis. After antituberculosis treatment with the 2HREZ/10HRE regimen, the patient's pain behind the sternum gradually alleviated, and the enlarged mediastinal lymph nodes gradually narrowed.
Pulmonary Langerhans cell histiocytosis presenting as an uncommon mass in the lung: A rare case report and literature review
Authors: Zhang Tingxiu , Hu Sheng , Teng Yue , Li Zhiwei , Xiao Zhenliang , Ma Lijie 
Published: August 2024

Key Clinical MessageA comprehensive diagnostic approach, including immunohistochemistry, is crucial for confirming pulmonary Langerhans cell histiocytosis in adults. Individualized treatment with dynamically adjusted chemotherapy based on therapeutic response leads to significant absorption of lesions and symptom alleviation. Regular follow‐up and timely treatment adjustments according to the patient's condition are essential in managing this rare disease.
A Perspective on Aldoximes as Platform Chemicals: Rethinking C−N Bond Formation
Authors: Wegener Hannes W. , Diekamp Justus , Seidensticker Thomas 
Published: August 2024

AbstractPrimary amines and nitriles play a central role in almost every branch of the chemical industry, and many synthetic strategies exist to obtain them. The major drawbacks of these existing approaches are low selectivities and highly toxic reagents like hydrogen cyanide. This article presents alternative catalytic routes based on aldoximes to address those challenges and outlines what needs to be done to implement aldoximes into a greener amine and nitrile production.
Consequences of the Pb−S Bond Formation for Lead Halide Perovskites
Authors: Wierzbowska Małgorzata , Wojtkowiak Kamil , Mikłas Alicja , Jezierska Aneta 
Published: August 2024

AbstractLead halide perovskites are structurally not stable due to their ionic bonds. Using sulfur agents in the crystal growth improves the stability and performance of the photovoltaic and light‐emitting devices. In this theoretical work, we use a small toy S‐radical in place of A cation in the bulk of lead iodide perovskite, and highlight the significance of the Pb−S covalent‐double‐bond formation for: the charge redistribution on the neighboring bonds that also turn to be covalent, phase transformation to a stable non‐perovskite structure, and superior optoelectronic properties. The chemical analysis was performed with the Quantum Theory of Atoms In Molecules (QTAIM) and Non‐Covalent Interactions (NCI) index. Excitonic properties were obtained from the solution of ab initio Bethe–Salpeter equation. Presence of the spin‐orbit coupling triggers an interplay between the Frenkel and charge‐transfer multiexcitons, switching between the photovoltaic and laser applications. Multiexcitons obey the exciton‐fission preconditions.
Gold Meets Selenium: Dual Activation of Selenium‐Containing Propargyl Alcohols Towards the Synthesis of 2H‐Chromenes and Mechanistic Insights
Authors: Lopes Eric F. , Dietl Martin C. , Ziegler Benjamin , Rudolph Matthias , Barcellos Thiago , Oeser Thomas , Lüdtke Diogo S. , Hashmi A. Stephen K. 
Published: August 2024

AbstractHerein, we report the synthesis of seleno‐substituted chromenes from selenoalkynes and phenols. In this cascade reaction, the applied gold catalyst not only functions as a π‐acid, but also as a Lewis acid, enabling the propargylic substitution in the first step to connect the oxygen carbon bond. Under the optimal reaction condition a total of 26 chromenes were accessible by this modular access. During scale up experiments, the hydrolysis of the vinylselenium substructure to the corresponding chromenones was observed. By revisiting the electron‐rich starting materials, four chromenones were produced following a one‐pot reaction using a single gold catalyst. To better understand the interaction of gold and selenium, a series of nuclear magnetic resonance studies and high‐resolution mass spectrometry studies were performed, which led to the proposal of a mechanism for this transformation.
Bereaved parent preferences on quality end‐of‐life care for children with cancer in the South
Authors: Martinez Isaac , Currie Erin , Davis Elizabeth S. , Kumar Rohail , Lawhon Valerie , Snaman Jennifer M. , Tefera Raba B. , Bhatia Smita , Rosenberg Abby R. , Johnston Emily E. 
Published: August 2024

AbstractPurposeThe authors sought to understand bereaved family preferences for end‐of‐life (EOL) care, particularly among Black families and those in the South.MethodsSemi‐structured interviews were conducted with parents of children who died of cancer ≥6 months before at Children’s of Alabama. Themes were identified via content analysis. Quotes related to medical intensity, chemotherapy, and location of death (LOD) were scored on 5‐point Likert scales, ranging from 1 (comfort care, chemotherapy, or home death) to 5 (medically intense care, avoidance of chemotherapy, or hospital death).ResultsTwenty‐seven bereaved parents (12 Black) were interviewed. Children died at a mean of 13.1 years (SD = 6.1 years) and a median of 3 years before the interview (range = 1–12 years). Ten children (42%) had central nervous system tumors and the majority (63%) died in the hospital. Family decision‐making involved maintaining hope, not causing harm, doing what was best for their child and themselves, and religious beliefs. There was no clear preference for home versus hospital death (3.0 [1.8–4.0]). Instead, parents considered their child’s desires and/or medical needs, siblings, and prior experiences with death. To have a comfortable death, parents highlighted the need for comprehensive education about their child’s EOL, a caring and comfortable environment, and 24/7 access to their care team. Families expressed a dual preference for comfort care (1.8 [1.3–2.8]) and chemotherapy (3.5 [2.7–4.1]) at EOL.ConclusionsFamilies did not see chemotherapy and comfort care as conflicting goals. They sought quality care emphasizing flexibility, quality time with their child, and open access to their care team, regardless of LOD.
AICyte‐alone capabilities as an independent screener for triaging cervical cytology using a 50% negative cutoff value
Authors: Zeng Xianxu , Starr David , Li Juan , Bi Xuejie , Wang Chun , Bai Xinru , Yin Yanxue , Wu Xue , Wei Jingjing , Du Hui , Dai Wenkui , Li Changzhong , Wu Xiangchen , Wu Ruifang , Zhao Chengquan 
Published: August 2024

AbstractBackgroundAICyte has previously demonstrated a potential role in cervical cytology screening for reducing the workload by using a 50% negative cutoff value. The aim of the current study is to evaluate this hypothesis.MethodsThe authors used the Ruiqian WSI‐2400 (with the registered trademark AICyte) to evaluate a collection of 163,848 original cervical cytology cases from 2018 to 2023 that were collected from four different hospital systems in China. A breakdown of cases included 46,060 from Shenzhen, 67,472 from Zhengzhou, 25,667 from Shijiazhuang, and 24,649 from Jinan. These collected cases were evaluated using the AICyte system, and the data collected were statistically compared with the original interpretative results.ResultsIn 98.80% of all artificial intelligence cases that were designated as not needing further review, the corresponding original diagnosis was also determined to be negative. For any cases that were designated atypical squamous cells, cannot exclude high‐grade squamous intraepithelial lesion or higher, the sensitivity and negative predictive value were 90.77% and 98.80%, respectively. The sensitivity and negative predictive value were greater in cases designated as low‐grade squamous intraepithelial lesion or higher at 98.92% and 99.94%, respectively. Of the 49 low‐grade squamous intraepithelial lesion or higher that were designed by AICyte as not needing further review, the cytohistologic correlation revealed eight cases of cervical intraepithelial neoplasia 1 and 18 negative cases; and the remaining cases were without histologic follow‐up. In practice, AICyte used at a 50% negative cutoff value could reduce the anticipated workload if a protocol were implemented to label cases that qualified within the negative cutoff value as not needing further review, thereby finalizing the case as negative for intraepithelial lesions and malignancy.ConclusionsFor pathologic practices that do not have cytotechnologists or in which the workflow is sought to be optimized, the artificial intelligence system AICyte alone to be an independent screening tool by using a 50% negative cutoff value, which is a potential assistive method for cervical cancer screening.
Exploring the Impact of Ni Doping in Tuning the Bandgap, Electronic, Optoelectronic and Photocatalytic Properties of ZnFe2O4
Authors: Chatterjee Karabi , Gorai Anupam , Hait Swarnali , Maity Subrata , Baskey Sen Moni , Dutta Arpita , Nag Riya , Bera Abhijit , Sarkar Sanjit , Saha Sudip K. , Jahid Akhtar Abu 
Published: August 2024

AbstractThe exploration of semiconductor nanostructures utilizing mixed metal materials is an emerging area of study across fields including field‐effect transistors, chemical sensors, photodetectors, photocatalysts, and many more. In this study, Schottky diodes based on ZnFe2O4 (ZFO) were constructed with an aim to tune their electronic and optoelectronic characteristics. Here, Ni doping facilitated the tuning of electronic properties, leading to a significant increase in the rectification ratio from 238 to 1172, along with a reduction in the potential barrier height from 0.67 V to 0.65 V. This is attributed to Ni's role as a charge carrier in ZFO, enhancing carrier concentration, confirmed by Mott‐Schottky analysis. The 5 mol % Ni‐doped ZFO also exhibited remarkable light sensitivity, with its rectification ratio surging to 1795 under illumination, four times that of the undoped version. Additionally, its photo‐sensitivity soared to 42.46 %, nearly quadrupling the undoped device's performance, and its power gain impressively climbed to 38.4 %, which is over twelvefold the undoped sample's output. Furthermore, the diode responds strongly to optical illumination, making this structure suitable for use as a photodiode or photosensor. Apart from that by employing a doping strategy, we achieved 64.61 % degradation of methylene blue dye under visible light in 120 minutes, compared to 36.85 % for the undoped sample. These indicate that hydrothermally synthesized Ni‐doped ZFO is promising for visible light‐driven multifunctional applications.