bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing metabolic efficiency via novel constitutive promoters to produce protocatechuic acid in Escherichia coli
Authors: Örn Oliver Englund , Hagman Arne , Ismail Mohamed , Leiva Eriksson Nélida , Hatti-Kaul Rajni 
Published: August 2024

Abstract
The antioxidant molecule protocatechuic acid (PCA) can also serve as a precursor for polymer building blocks. PCA can be produced in Escherichia coli overexpressing 3-dehydroshikimate dehydratase (DSD), an enzyme that catalyses the transformation of 3-dehydroshikimate to PCA. Nevertheless, optimizing the expression rate of recombinant enzymes is a key factor in metabolic engineering when producing biobased chemicals. In this study, a degenerate synthetic promoter approach was investigated to improve further the production of PCA. By limited screening of a randomized promoter library made using pSEVA221 plasmid in E. coli, three novel synthetic constitutive promoters were selected that increased the PCA yield from glucose by 10–21% compared to the inducible T7-promoter. RT-qPCR analysis showed that the DSD gene, regulated by the synthetic promoters, had high expression during the exponential phase, albeit the gene expression level dropped 250-fold during stationary phase. Besides the increased product yield, the synthetic promoters avoided the need for a costly inducer for gene expression. Screening of the entire promoter library is likely to provide more positive hits. The study also shows that E. coli transformed with the DSD gene on either pSEVA221 or pCDFDuet plasmids exhibit background PCA levels (~ 0.04 g/L) in the absence of a transcriptional regulatory element.

Key points
• Degenerate synthetic promoters are remarkable tools to produce protocatechuic acid.
• The constitutive synthetic promoters did not affect the growth rate of the bacterial host.
• The use of constitutive synthetic promoters avoids the need for the costly inducer.
Assessing the Environmental Impacts, Condition and Sustainability of Mountain Biking Trails in an Urban National Park
Authors: Smith Isabella , Pickering Catherine Marina 
Published: August 2024

AbstractMountain biking is a popular recreational activity in natural areas, with thousands of formal trails designed, constructed and maintained by land managers. Increasingly, there are also rising numbers of informal trails created by riders. A challenge for land managers is identifying, assessing, and then mitigating environmental impacts created by trails, including in protected areas. Here we assessed mountain biking trails in a large, popular national park on the Gold Coast, Australia, addressing the currently limited research comparing the extent, environmental impacts, condition and sustainability of these trails. Impacts from the 31.4 km of formal and 33.7 km of informal trails through the forests in Nerang National Park (1659 ha) included soil erosion (16.48 m3) and loss of vegetation along and adjacent to the trails (90,955 m2). Formal trails were six times more popular and wider on average (1.1 m vs 0.7 m) than informal trails, but less incised than informal trails (4.6 cm deep vs 6.3 cm). Generalised Linear Models showed that Trail Grade, slope and alignment best-predicted trail condition, highlighting the importance of good trail design in minimising trail impacts. It is recommended most of the informal trails are closed and rehabilitated, as they were not well-designed, increase fragmentation and have environmental impacts, with some traversing ecologically sensitive areas. In addition, some formal trails need to be upgraded to deal with erosion and other impacts. More broadly, the increasing demand for mountain biking must be addressed, including exploring opportunities to promote areas outside of national parks while minimising environmental impacts and other challenges associated with the creation and use of informal mountain bike trails in protected areas.
Plume and wall temperature impact on the subsonic aft-body flow of a generic space launcher geometry
Authors: Kirchheck Daniel , Schumann Jan-Erik , Fertig Markus , Saile Dominik , Hannemann Volker , Eggers Thino , Gülhan Ali 
Published: August 2024

AbstractExperimental and numerical simulation of launcher base flows are crucial for future launcher design. In experiments, exhaust plume simulation is often limited to cold or slightly heated gases. In numerical simulations, multi-species reactive flow is often neglected due to limited resources. The impact of these simplifications on the relevant flow features, compared to real flight scenarios, needs to be characterized in order to enhance the design process. Experimental and numerical investigations were carried out within the framework of the SFB/TRR 40 Collaborative Research Centre to study the impact of plume and wall temperature on the base flow of a generic small-scale launcher configuration. Wind tunnel tests were performed in the Hot Plume Testing Facility (HPTF) at DLR, Cologne, using subsonic ambient flow and pressurized air or hydrogen–oxygen combustion as exhaust gases. The tests were numerically rebuilt using the DLR TAU code employing a scale-resolved IDDES approach, including thermal coupling and detailed chemistry. The paper combines the experimental and numerical findings from the SFB/TRR 40 base flow studies and highlights the most prominent influences on the mean flow field, the pressure field, the dynamic wake flow motion, and the resulting aerodynamic forces on the nozzle. High-frequency pressure measurements, high-speed schlieren recordings, and time-resolved CFD results are evaluated using spectral and modal analysis of the one- and two-dimensional flow field data.
Examining the impact of solid organ transplantation on family planning: pre- and post-transplantation pregnancy evaluations for both women and men
Authors: Böhm Lea , Schirm Nina , Zimmermann Tanja , Meyer Nadia , von Versen-Höynck Frauke 
Published: August 2024

Abstract
Purpose
The aim of this study was to collect and analyze information from pregnancies of organ transplanted women and partners of organ transplanted men. The goal was to enhance counseling regarding pregnancy planning and management and to enable more targeted monitoring to improve maternal and child health.

Methods
In this retrospective, multicenter cohort study, women and men aged 18 to 45 who had undergone organ transplantation in Germany, Austria, and Switzerland were surveyed about their pregnancies before and after transplantation by using a self-developed questionnaire.

Results
Even through transplanted women planned their pregnancies more carefully than before transplantation, they still experienced more pregnancy complications afterward. The live birth rate for pregnancies of partners of transplanted men, especially men who received a thoracic organ, was lower compared to before transplantation. Furthermore, this study showed that pregnancies of the partners of male transplant recipients occurred significantly less by spontaneous conception in comparison to pregnancies of transplanted women.

Conclusion
Pregnancies after organ transplantation are possible but associated with an increased risk of pregnancy complications. Therefore, early counseling for transplanted women and men who wish to have children, along with extensive monitoring during pregnancy, is necessary.
Spatial arrangement, polarity, and posttranslational modifications of the microtubule system in the Drosophila eye
Authors: Kos Piotr , Baumann Otto 
Published: August 2024

AbstractWe have analyzed the organization of the microtubule system in photoreceptor cells and pigment cells within the adult Drosophila compound eye. Immunofluorescence localization of tubulin and of Short stop, a spectraplakin that has been reported to be involved in the anchorage of microtubule minus ends at the membrane, suggests the presence of non-centrosomal microtubule-organizing centers at the distal tip of the visual cells. Ultrastructural analyses confirm that microtubules emanate from membrane-associated plaques at the site of contact with cone cells and that all microtubules are aligned in distal–proximal direction within the photoreceptor cells. Determination of microtubule polarities demonstrated that about 95% of the microtubules in photoreceptor cells are oriented with their plus end in the direction of the synapse. Pigment cells in the eye contain only microtubules aligned in distal–proximal direction, with their plus end pointing towards the retinal floor. There, two populations of microtubules can be distinguished, single microtubules and bundled microtubules, the latter associated with actin filaments. Whereas microtubules in both photoreceptor cells and pigment cells are acetylated and mono/bi-glutamylated on α-tubulin, bundled microtubules in pigment cells are apparently also mono/bi-glutamylated on β-tubulin, providing the possibility of binding different microtubule-associated proteins.
The influence of vertebrate scavengers on leakage of nutrients from carcasses
Authors: Wenting Elke , Jansen Patrick A. , Burggraeve Simon , Delsman Devon F. , Siepel Henk , van Langevelde Frank 
Published: August 2024

AbstractThe decomposition of carcasses by scavengers and microbial decomposers is an important component of the biochemical cycle that can strongly alter the chemical composition of soils locally. Different scavenger guilds are assumed to have a different influence on the chemical elements that leak into the soil, although this assumption has not been empirically tested. Here, we experimentally determine how different guilds of vertebrate scavengers influence local nutrient dynamics. We performed a field experiment in which we systematically excluded different subsets of vertebrate scavengers from decomposing carcasses of fallow deer (Dama dama), and compared elemental concentrations in the soil beneath and in the vegetation next to the carcasses over time throughout the decomposition process. We used four exclusion treatments: excluding (1) no scavengers, thus allowing them all; (2) wild boar (Sus scrofa); (3) all mammals; and (4) all mammals and birds. We found that fluxes of several elements into the soil showed distinct peaks when all vertebrates were excluded. Especially, trace elements (Cu and Zn) seemed to be influenced by carcass decomposition. However, we found no differences in fluxes between partial exclusion treatments. Thus, vertebrate scavengers indeed reduce leakage of elements from carcasses into the soil, hence influencing local biochemical cycles, but did so independent of which vertebrate scavenger guild had access. Our results suggest that carcass-derived elements are dispersed over larger areas rather than locally leak into the soil when vertebrate scavengers dominate the decomposition process.
Sharp Sobolev inequalities on noncompact Riemannian manifolds with $$\textsf{Ric}\ge 0$$ via optimal transport theory
Authors: Kristály Alexandru 
Published: August 2024

AbstractIn their seminal work, Cordero-Erausquin, Nazaret and Villani (Adv Math 182(2):307-332, 2004) proved sharp Sobolev inequalities in Euclidean spaces via Optimal Transport, raising the question whether their approach is powerful enough to produce sharp Sobolev inequalities also on Riemannian manifolds. By using $$L^1$$

L
1

-optimal transport approach, the compact case has been successfully treated by Cavalletti and Mondino (Geom Topol 21:603-645, 2017), even on metric measure spaces verifying the synthetic lower Ricci curvature bound. In the present paper we affirmatively answer the above question for noncompact Riemannian manifolds with non-negative Ricci curvature; namely, by using Optimal Transport theory with quadratic distance cost, sharp $$L^p$$

L
p

-Sobolev and $$L^p$$

L
p

-logarithmic Sobolev inequalities (both for $$p>1$$

p
>
1

and $$p=1$$

p
=
1

) are established, where the sharp constants contain the asymptotic volume ratio arising from precise asymptotic properties of the Talentian and Gaussian bubbles, respectively. As a byproduct, we give an alternative, elementary proof to the main result of do Carmo and Xia (Math 140:818-826, 2004) and subsequent results, concerning the quantitative volume non-collapsing estimates on Riemannian manifolds with non-negative Ricci curvature that support Sobolev inequalities.
Development of MOF-derived Co3O4 microspheres composed of fiber stacks for simultaneous electrochemical detection of Pb2+ and Cu2+
Authors: Guo Jieli , Li Jin , Xing Xiujing , Xiong Wei , Li Hao 
Published: August 2024

AbstractAs an ideal transition metal oxide, Co3O4 is a P-type semiconductor with excellent electrical conductivity, non-toxicity and low cost. This work reports the successful construction of Co3O4 materials derived from metal-organic frameworks (MOFs) using a surfactant micelle template-solvothermal method. The modified electrodes are investigated for their ability to electrochemically detect Pb2+ and Cu2+ in aqueous environments. By adjusting the mass ratios of alkaline modifiers, the morphological microstructures of Co3O4-X exhibit a transition from distinctive microspheres composed of fiber stacks to rods. The results indicate that Co3O4-1(NH4F/CO(NH2)2 = 1:0) has a distinctive microsphere structure composed of stacked fibers, unlike the other two materials. Co3O4-1/GCE is used as the active material of the modified electrode, it shows the largest peak response currents to Pb2+ and Cu2+, and efficiently detects Pb2+ and Cu2+ in the aqueous environment individually and simultaneously. The linear response range of Co3O4-1/GCE for the simultaneous detection of Pb2+ and Cu2+ is 0.5–1.5 μM, with the limits of detection (LOD, S/N = 3) are 9.77 nM and 14.97 nM, respectively. The material exhibits a favorable electrochemical response, via a distinctive Co3O4-1 microsphere structure composed of stacked fibers. This structure enhances the number of active adsorption sites on the material, thereby facilitating the adsorption of heavy metal ions (HMIs). The presence of oxygen vacancies (OV) can also facilitate the adsorption of ions. The Co3O4-1/GCE electrode also exhibits excellent anti-interference ability, stability, and repeatability. This is of great practical significance for detecting Pb2+ and Cu2+ in real water samples and provides a new approach for developing high-performance metal oxide electrochemical sensors derived from MOFs.
Graphical abstract
The akinetic crisis in Parkinson´s disease- the upper end of a spectrum of subacute akinetic states
Authors: Pötter-Nerger Monika , Schrader Christoph , Jost Wolfgang H. , Höglinger Günter 
Published: August 2024

AbstractThe akinetic crisis is defined as an acute, potentially life-threatening, levodopa-resistant, severe aggravation of rigidity, severe akinesia, associated with high fever, disturbance of consciousness, dysphagia and autonomic symptoms often due to disruption of dopaminergic medication or infections. The akinetic crisis is a relatively rare event, however subacute mild-moderate motor symptom deterioration in Parkinson´s disease (PD) patients is a frequent cause of hospitalization. In this review, we propose that the akinetic crisis is the upper end of a continuous spectrum of acute akinetic states depending on the degree of the progressive levodopa-resistance. Clinical symptomatology, risk factors, and instrumental diagnostics as the DAT-SPECT reflecting a biomarker of levodopa-resistance will be discussed to evaluate the spectrum of akinetic states. Pathophysiological considerations about the potential role of proinflammatory cytokines on the progressive levodopa-resistance will be discussed and therapeutical, consensus-based guidelines will be presented.
Depression, higher level of tension induction, and impaired coping strategies in response to stress in women with PCOS correlate with clinical and laboratory indices of hyperandrogenism and not with central obesity and insulin resistance
Authors: Dutkiewicz Edyta , Rachoń Dominik , Dziedziak Miłosz , Kowalewska Agnieszka , Moryś Joanna 
Published: August 2024

Abstract
PCOS is characterized by ovarian hyperandrogenism and insulin resistance (IR), which give rise to symptoms of hyperandrogenism and central obesity, which in turn may cause depression, lower self-esteem, and deteriorate coping strategies in stressful situations.

The purpose
to examine the mental condition, self-esteem, and ways of coping with stress in women with PCOS compared to age and BMI-matched healthy controls and to correlate them with clinical and laboratory hyperandrogenism, central obesity, and IR.

Methods
42 women with PCOS and 39 controls were assessed for the above-mentioned psychological measures and correlated with serum hormonal and metabolic parameters.

Results
Compared to controls, women with PCOS had more symptoms of depression (p = 0.026), a higher level of tension induction (p = 0.032), were more prone to alcohol consumption (p = 0.015), and were less likely to use the strategy of active coping in stressful situations (p = 0.014) and to seek instrumental (p = 0.048) and emotional support (p = 0.043). The presence of hirsutism correlated negatively with the level of emotional induction (R = -0.32, p < 0.05), and androgenic alopecia positively with the hedonistic tone (R = 0.36, p < 0.05). Serum testosterone (TST) correlated positively with the likelihood of seeking instrumental support in stressful situations (R = 0.31, p < 0.05) and with emotional focus (R = 0.34, p < 0.05). Serum androstenedione (A4-dione) correlated negatively with the escape behavior (R = -0.32, p < 0.05). No correlations were found between waist circumference and IR with the studied psychological measures.

Conclusions
Women with PCOS are characterized by depression, higher levels of tension induction, and impaired coping strategies in stressful situations, which correlate with clinical and laboratory indices of hyperandrogenism and not with central obesity and IR.
Neuroretinal and microvascular retinal features in dementia with Lewy body assessed by optical coherence tomography angiography
Authors: Albanese Giuseppe Maria , Gharbiya Magda , Visioli Giacomo , Panigutti Massimiliano , Margarella Andrea , Romano Enrico , Mastrogiuseppe Elvia , Sepe-Monti Micaela , Bruno Giuseppe , D’Antonio Fabrizia 
Published: August 2024

Abstract
Objective
To explore retinal changes in patients with Dementia with Lewy Bodies (DLB) using Spectral Domain-Optical Coherence Tomography (SD-OCT) and Optical Coherence Tomography Angiography (OCTA), aiming to identify potential biomarkers for diagnosis and monitoring.

Methods
A cross-sectional study analyzed 15 DLB patients and 18 matched controls. Participants underwent physical, neurological, neuropsychological, and ophthalmological evaluations, including SD-OCT and OCTA. Logistic regression, adjusted for age, sex, and inter-eye correlation, was employed to identify retinal alterations in patients affected by DLB.

Results
OCTA revealed that DLB is associated with reduced superficial and deep vessel densities (SVD and DVD) in the macula (p < 0.01), as well as decreased peripapillary vessel density (ppVD, p < 0.01). SD-OCT parameters showed correlations with DLB, including reduced central macular thickness (CMT, p < 0.001) and thinning of the ganglion cell layer-inner plexiform layer (GCL-IPL, p < 0.01). Logistic regression (R²=0.26) identified reduced ppVD as a significant predictor of DLB (p = 0.030).

Conclusions
Impairments in retinal capillaries, especially lower ppVD, might mirror cerebral hypoperfusion in DLB, potentially due to reduced Vascular Endothelial Growth Factor (VEGF) levels and increased α-synuclein. Further investigations are warranted to confirm the causal relationship between these observations, disease severity, and progression, as well as their potential role as biomarkers for DLB.
The differential illusion memory for high-associated abstract concepts (DIM-HA) effect
Authors: Marín-Gutiérrez Alejandro , Díez Villoria Emiliano , González Martín Ana María 
Published: August 2024

AbstractA vast body of evidence has shown that concrete concepts are processed faster and more accurately than abstract concepts in a variety of cognitive tasks. This phenomenon is widely known as the concreteness effect, and explanations for its occurrence seem to reflect differences in processing and organization for both types of representations. While there is considerable evidence to support this concreteness effect, the nature of these differences is still controversial. In developing an explanation, we have proposed a relatively different approach from a false memory perspective using the Deese–Roediger–McDermott paradigm. To explore the implications of the association in creating false memories, we explore behavioral and electrophysiologically the false memory effect, where targets were manipulated according to their association strength and their concreteness. Results showed that false recognition rates differed significantly between concrete and abstract critical words when they were associated strongly with their respective lists, which led to a higher proportion of abstract false alarms both in behavioral and electrophysiological experiments. The principal outcome, which was called the DIM-HA effect, was discussed in terms of theories of associative activation and qualitatively different representation.
Morphology and taphonomy of the gastropod Terebralia palustris from an iron age site in the Arabian Peninsula
Authors: Müller García Inés de la Fortuna , Nebelsick James H. 
Published: August 2024

AbstractThe Indo-Pacific gastropod Terebralia palustris is particularly suitable for comparing natural and anthropogenic induced taphonomic pathways due to its wide geographic distribution and common presence within archeological context. The present study aims to (1) correlate shell architecture and morphology with fragmentation pattern and preservation, (2) quantify taphonomic changes to differentiate between natural vs. anthropogenic preservation features, (3) provide a guideline for analyzing fragmented shell remains in archeological material. Shells and taphonomic features were studied from both recent mangrove environments from the Emirate of Sharjah, United Arab Emirates as well as archeological material within the Iron age II site (1000–600 BC) of Muweilah near the City of Sharjah. Techniques utilized include morphometry, thin sectioning, scanning electron microscopy (SEM) of recent specimens and a semi—quantitative taphonomic analysis of anthropogenic material. Thin sectioning shows a complex internal shell morphology with a tripartite subdivision of shell layers. The recent material shows better preserved features on both the exterior and internal shell surfaces than the highly fragmented material recovered from the archeological context, which shows a distinct size distribution as well as showing higher levels of surface abrasion, surface cracks and color alterations. These features are correlated to extraction techniques, cooking methods and waste disposal handling.