bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene expression response under thermal stress in two Hawaiian corals is dominated by ploidy and genotype
Authors: Chille Erin E. , Stephens Timothy G. , Misri Deeksha , Strand Emma L. , Putnam Hollie M. , Bhattacharya Debashish 
Published: July 2024

AbstractTranscriptome data are frequently used to investigate coral bleaching; however, the factors controlling gene expression in natural populations of these species are poorly understood. We studied two corals, Montipora capitata and Pocillopora acuta, that inhabit the sheltered Kāne'ohe Bay, Hawai'i. M. capitata colonies in the bay are outbreeding diploids, whereas P. acuta is a mixture of clonal diploids and triploids. Populations were sampled from six reefs and subjected to either control (no stress), thermal stress, pH stress, or combined pH and thermal stress treatments. RNA‐seq data were generated to test two competing hypotheses: (1) gene expression is largely independent of genotype, reflecting a shared treatment‐driven response (TDE) or, (2) genotype dominates gene expression, regardless of treatment (GDE). Our results strongly support the GDE model, even under severe stress. We suggest that post‐transcriptional processes (e.g., control of translation, protein turnover) modify the signal from the transcriptome, and may underlie the observed differences in coral bleaching sensitivity via the downstream proteome and metabolome.
Bumblebees mediate landscape effects on a forest herb's population genetic structure in European agricultural landscapes
Authors: Feigs Jannis Till , Huang Siyu , Holzhauer Stephanie I. J. , Brunet Jörg , Diekmann Martin , Hedwall Per‐Ola , Kramp Katja , Naaf Tobias 
Published: July 2024

AbstractSpatially isolated plant populations in agricultural landscapes exhibit genetic responses not only to habitat fragmentation per se but also to the composition of the landscape matrix between habitat patches. These responses can only be understood by examining how the landscape matrix influences among‐habitat movements of pollinators and seed vectors, which act as genetic linkers among populations. We studied the forest herb Polygonatum multiflorum and its associated pollinator and genetic linker, the bumblebee Bombus pascuorum, in three European agricultural landscapes. We aimed to identify which landscape features affect the movement activity of B. pascuorum between forest patches and to assess the relative importance of these features in explaining the forest herb's population genetic structure. We applied microsatellite markers to estimate the movement activity of the bumblebee as well as the population genetic structure of the forest herb. We modelled the movement activity as a function of various landscape metrics. Those metrics found to explain the movement activity best were then used to explain the population genetic structure of the forest herb. The bumblebee movement activity was affected by the cover of maize fields and semi‐natural grasslands on a larger spatial scale and by landscape heterogeneity on a smaller spatial scale. For some measures of the forest herb's population genetic structure, that is, allelic richness, observed heterozygosity and the F‐value, the combinations of landscape metrics, which explained the linker movement activity best, yielded lower AICc values than 95% of the models including all possible combinations of landscape metrics.Synthesis: The genetic linker, B. pascuorum, mediates landscape effects on the population genetic structure of the forest herb P. multiflorum. Our study indicates, that the movement of the genetic linker among forest patches, and thus the pollen driven gene flow of the herb, depends on the relative value of floral resources in the specific landscape setting. Noteworthy, the population genetic structure of the long‐lived, clonal forest herb species correlated with recent land‐use types such as maize, which have been existing for not more than a few decades within these landscapes. This underscores the short time in which land‐use changes can influence the evolutionary potential of long‐lived wild plants.
FeCl3 Catalyzed Synthesis of cis‐Fused Reduced THC Analogues
Authors: Gurgone Lucía , Adessi Tonino G. , La‐Venia Agustina , Gandon Vincent , Riveira Martín J. 
Published: July 2024

AbstractStudies on a cycloisomerization reaction of 2‐prenylated‐2H‐pyrans towards cis‐fused tetrahydro‐6H‐benzo[c]chromene systems (reduced cis‐THC analogues) are reported. The transformation is catalyzed by iron chloride and proceeds with the formation of two new bonds and two stereocenters diastereoselectively. The same catalyst also promotes the formation of these products via a one‐pot tandem Knoevenagel condensation of 1,3‐cyclohexanediones and citral or farnesal aldehydes, followed by cycloisomerization establishing a direct assembly of this important scaffold.
Were fewer boys born in the United States during the early months of the COVID‐19 pandemic? A test of the Trivers–Willard hypothesis
Authors: Cleaver Peyton , Non Amy L. 
Published: July 2024

AbstractThe Trivers–Willard hypothesis predicts that mammalian parents in poor environmental conditions will favor the offspring sex with more reliable chance of reproductive success, which in humans is females. Three months following the onset of the COVID‐19 pandemic in South Africa, England, and Wales, there were significant decreases in the sex ratio at birth (SRB) (male births/total live births). We analyzed this ratio with a seasonal autoregressive moving average model, and a logistic regression, using nationwide natality data for all singleton births in the United States from 2015 to 2021 (n = 25,201,620 total births). We identified no significant change in the sex ratio in either analysis. Rather, we observed marked differences in the sex ratio by maternal characteristics of race/ethnicity, age, and education, with more vulnerable groups having lower sex ratios. These findings suggest the SRB may be an important marker of reproductive vulnerability for disadvantaged groups in the United States.
The clinical manifestations and treatment outcomes of Behçet's disease: A single‐center experience
Authors: Hama Hussein Karokh F. , Abdullah Hiwa O. , Ahmed Shaho F. , Qadir Aso N. , Asaad Hoshmand R. , Fattah Fattah H. , Hama Amin Bnar J. , Gharib Dana T. , Mohammed Shvan H. , Salih Rawezh Q. , Abdalla Berun A. , Kakamad Fahmi H. , Mohammed Shorsh A. 
Published: July 2024

AbstractBackground and AimsBehçet's disease is a chronic, multisystemic, and relapsing inflammatory disorder. It lacks a permanent cure, the focus of treatment is on mitigating symptoms, decreasing the frequency and severity of relapses, and preventing life‐threatening complications. This study aims to report the experience of a single center in managing patients with Behçet's disease and discuss the treatment outcomes.MethodsThis study was a retrospective case series conducted over 2 years. All cases were clinically diagnosed according to the International Criteria for Behçet's Disease. The extracted data were demographics, family history, clinical findings, criteria scores, treatment, and outcomes.ResultsA total of 31 patients were included, consisting of 13 males (42%) and 18 females (58%). Most cases were over the age of 30, and both genders were nearly equally distributed among age groups. The most commonly affected site was the oral cavity, observed in 96.77% of cases. Genital, cutaneous, and vascular involvements were more common in males, while females were more likely to have oral, ocular, and musculoskeletal involvements. For various treatment regimens, oral, cutaneous, vascular, and musculoskeletal involvements showed complete response in all cases. Among cases with genital involvement, complete response was achieved in seven cases (41.2%), while four cases (23.5%) showed only partial response, and six cases (35.3%) experienced recurrence. In cases with ocular involvement, only partial responses were observed.ConclusionOral, cutaneous, vascular, and musculoskeletal involvements may have a higher likelihood of a complete response to treatment regimens. However, genital involvement may be the most recurrent manifestation, followed by ocular involvement.
A comparison between Hankel and Fourier methods for photothermal radiometry analysis
Authors: Sheikh Raza , Pompidou Quentin , Villarreal Ezekiel , Horny Nicolas , Ban Heng 
Published: July 2024

AbstractPhotothermal radiometry has recently been investigated for use in the multidimensional thermal characterization of anisotropic samples. In application, there are two principal thermal models available for such characterization: a Cartesian model for the heat equation, which requires the application of three Fourier transforms to arrive at a solution (dubbed the Fourier technique), and a cylindrical model for the heat equation, which requires the application of a Hankel transform and a single Fourier transform (dubbed the Hankel technique). The Fourier technique allows for three‐dimensional characterization, while the Hankel technique is expected to greatly reduce the computational time required. As these models can be very computationally expensive, the potential to reduce this cost is of great interest. In this work, these multidimensional models are presented after which they are compared for accuracy, computational time, and assumption limitations. It was found that both the Fourier and Hankel techniques could accurately arrive at desired thermal properties, but that the Hankel Technique reduced the computational time by between 100× and 250× depending upon mesh spacings. Accuracy limitations were found as the eccentricity of the heating laser was increased with a less than 13% error being induced from a beam with a 3–1 axis ratio. The Hankel technique shows ideal application in computationally expensive models which employ a relatively circular beam shape.
Association of opium use and tobacco smoking with α‐, β‐, and γ‐human papillomavirus oral infection
Authors: Karimi Abbas , Mohebbi Elham , Mckay‐Chopin Sandrine , Hadji Maryam , Rashidian Hamideh , Marzban Maryam , Naghibzadeh‐Tahami Ahmad , Gholipour Mahin , Eslami Hadi , Kamangar Farin , Tommasino Massimo , Gheit Tarik , Zendehdel Kazem 
Published: July 2024

AbstractHead and neck squamous cell carcinomas (HNSCCs) are linked to tobacco smoking, opium use, and human papillomavirus (HPV) infection. However, little is known about the association of HPV infection with risk factors of HNSCCs, including opium and tobacco use. This cross‐sectional analysis of a national multi‐center case–control study in Iran included 498 HNSCC cases and 242 controls. We investigated the association of opium and tobacco use with α‐ (n = 21), β‐ (n = 46), and γ‐HPV (n = 52) types in saliva samples using type‐specific bead‐based multiplex genotyping assays (TS‐MPG). We found that α‐HPV positivity was significantly associated with tobacco smoking (OR = 10.35; 95% CI = 1.15, 93; p = .03), but not with opium use (OR = 1.06; 95% CI = 0.41, 2.76; p = .89). Additionally, tobacco smoking correlated with an elevated risk of β‐species 2 HPV infection (OR = 1.28; 95% CI = 1.04, 1.58; p = .020). Conversely, opium use showed a positive association with γ‐species 12 HPV infection (OR = 5.67; 95% CI = 1.43, 22.44; p = .013). These findings indicate that tobacco and opium use may influence the risk of HPV infection in different ways depending on the HPV genus and species. Further studies are needed to replicate these findings in other populations.
Analyzing the role of ferroptosis in ribosome‐related bone marrow failure disorders: From pathophysiology to potential pharmacological exploitation
Authors: Papadimitriou‐Tsantarliotou Aliki , Avgeros Chrysostomos , Konstantinidou Maria , Vizirianakis Ioannis S. 
Published: July 2024

AbstractWithin the last decade, the scientific community has witnessed the importance of ferroptosis as a novel cascade of molecular events leading to cellular decisions of death distinct from apoptosis and other known forms of cell death. Notably, such non‐ apoptotic and iron‐dependent regulated cell death has been found to be intricately linked to several physiological processes as well as to the pathogenesis of various diseases. To this end, recent data support the notion that a potential molecular connection between ferroptosis and inherited bone marrow failure (IBMF) in individuals with ribosomopathies may exist. In this review, we suggest that in ribosome‐related IBMFs the identified mutations in ribosomal proteins lead to changes in the ribosome composition of the hematopoietic progenitors, changes that seem to affect ribosomal function, thus enhancing the expression of some mRNAs subgroups while reducing the expression of others. These events lead to an imbalance inside the cell as some molecular pathways are promoted while others are inhibited. This disturbance is accompanied by ROS production and lipid peroxidation, while an additional finding in most of them is iron accumulation. Once lipid peroxidation and iron accumulation are the two main characteristics of ferroptosis, it is possible that this mechanism plays a key role in the manifestation of IBMF in this type of disease. If this molecular mechanism is further confirmed, new pharmacological targets such as ferroptosis inhibitors that are already exploited for the treatment of other diseases, could be utilized to improve the treatment of ribosomopathies.
Análisis taxonómico de Passiflora cubensis Urb.
Authors: Gongora María Elvira Duharte 
Published: July 2024

ResumenSe analizan especímenes determinados como Passiflora cubensis Urb. y se comparan mediante análisis biométricos con el lectotipo, obteniéndose diferencias significativas en la morfología de las hojas v flores que junto a los resultados del estudio epidérmico y atendiendo a la distribución geográfica permiten la separación de una nueva subespecie y una nueva especie.
Queer, transgender, Black, and/or Indigenous people of color experiences in substance use disorder counseling: Enhancing QTBIPOC counseling care
Authors: Gorritz FitzSimons Frank B. , Byrd Jacklyn J. 
Published: July 2024

AbstractThis PhotoVoice study explored the lived experiences of queer, transgender, Black, and Indigenous people of color (QTBIPOC) in substance use disorder counseling. Furthermore, this study utilized a Critical Participatory Action Research framework to discuss QTBIPOC strategies and ideas on enhancing substance use disorder counseling for QTBIPOC communities. The group dialogues in this study further explored how QTBIPOC experience minority stress and affirmative counseling experiences when seeking out counseling for substance use. Substance use disorder counselors and institutions can strongly benefit from the implications of this study as part of enhancing overall QTBIPOC counseling care.
Lisdexamfetamine dimesylate‐exposition in male rats during the peripubertal period impairs inflammatory mechanisms, antioxidant activity, and apoptosis process in kidneys of male pubertal rats
Authors: Honório da Silva João Vinícius , Erthal Rafaela Pires , Vercellone Isadora Chagas , Santos Dayane Priscila dos , Ferraz Camila Rodrigues , de Matos Ricardo Luís Nascimento , Gonçalves Luís Eduardo Duarte , Bracarense Ana Paula Frederico Rodrigues Loureiro , Verri Waldiceu Aparecido , Câmara Niels Olsen Saraiva , de Andrade Fábio Goulart , Fernandes Glaura Scantamburlo Alves 
Published: July 2024

AbstractLisdexamfetamine dimesylate (LDX) is a prodrug of dextroamphetamine, which has been widely recommended for the treatment of Attention‐Deficit/Hyperactivity Disorder (ADHD). There are still no data in the literature relating the possible toxic effects of LDX in the kidney. Therefore, the present study aims to evaluate the effects of LDX exposure on morphological, oxidative stress, cell death and inflammation parameters in the kidneys of male pubertal Wistar rats, since the kidneys are organs related to the excretion of most drugs. For this, twenty male Wistar rats were distributed randomly into two experimental groups: LDX group—received 11,3 mg/kg/day of LDX; and Control group—received tap water. Animals were treated by gavage from postnatal day (PND) 25 to 65. At PND 66, plasma was collected to the biochemical dosage, and the kidneys were collected for determinations of the inflammatory profile, oxidative status, cell death, and for histochemical, and morphometric analyses. Our results show that there was an increase in the number of cells marked for cell death, and a reduction of proximal and distal convoluted tubules mean diameter in the group that received LDX. In addition, our results also showed an increase in MPO and NAG activity, indicating an inflammatory response. The oxidative status showed that the antioxidant system is working undisrupted and avoiding oxidative stress. Therefore, LDX‐exposition in male rats during the peripubertal period causes renal changes in pubertal age involving inflammatory mechanisms, antioxidant activity and apoptosis process.
Covalent organic frameworks and metal–organic frameworks for sustainable adsorptive removal/extraction of dirty dozen chemicals: A review
Authors: Emmanuel Stephen Sunday , Aniekezie Favour Chizoba , Adesibikan Ademidun Adeola 
Published: July 2024

AbstractDirty dozen chemicals (DDCs) are a group of 12 extremely toxic chemicals that were recognized at the Stockholm convention in 2001 for their severe impact on the ecosystem and human health. Despite the embargo and restraint placed on DDCs usage, these chemicals continue to find their way into the ecosystem because they are still secretly or openly applied by many nations, especially in African regions. Moreover, DDCs can still be perceived where they have been employed previously before the Stockholm convention treaty due to their persistent profile. This study aimed to critically review original works directed toward the removal of various dirty dozen chemicals using covalent and metal–organic frameworks (COFs and MOFs). Specifically, in this study, various COFs/MOFs and their composites with remarkably tailored adsorptive profiles are evaluated for their adsorption efficiency for different DDCs. In addition, the effect of various operating parameters that are of importance to environmentalists and various stakeholders for optimization purposes was empirically discussed. This review also fills knowledge vacuums about the COF/MOF‐DDCs adsorption process, offers insights into their reusability potential, fundamental mechanism, isotherm, and kinetic modeling, and offers a framework for future studies. Findings from this study revealed that COF and MOF have high DDC removal capacity and reusability potential attributed to their admirable porosity and the existence of a plethora of oxygen‐rich functional groups that allow for better interactions with DDCs through chelation, halogen bonding, H‐bonding, and π‐π interactions and stacking. This points to the upscaling potential of this remediation technique. Future researchers need to direct more efforts to the use of density functional theory for mechanism interpretation, exploration of hybrid technology, cost analysis, scalability, isotherm, thermodynamics, adsorption, and desorption kinetic modeling.
Extracellular vesicle surface display of αPD‐L1 and αCD3 antibodies via engineered late domain‐based scaffold to activate T‐cell anti‐tumor immunity
Authors: Chen Rui , Kang Ziqin , Li Wenhao , Xu Tianshu , Wang Yongqiang , Jiang Qiming , Wang Yuepeng , Huang Zixian , Xu Xiaoding , Huang Zhiquan 
Published: July 2024

AbstractExtracellular vesicles (EVs) are emerging as promising carriers for the delivery of therapeutic biologics. Genetic engineering represents a robust strategy for loading proteins of interest into EVs. Identification of EV‐enriched proteins facilitates protein cargo loading efficiency. Many EV‐enriched proteins are sorted into EVs via an endosomal sorting complex required for transport (ESCRT)‐dependent pathway. In parallel, viruses hijack this EV biosynthesis machinery via conserved late domain motifs to promote egress from host cells. Inspired by the similarity of biogenesis between EVs and viruses, we developed a synthetic, Late domain‐based EV scaffold protein that enables the display of a set of single chain variable fragments (scFvs) on the EV surface. We named this scaffold the Late domain‐based exosomal antibody surface display platform (LEAP). We applied the LEAP scaffold to reprogramme HEK293T cell‐derived EVs to elicit T‐cell anti‐tumor immunity by simultaneously displaying αPD‐L1 and αCD3 scFvs on the EV surface (denoted as αPD‐L1×αCD3 bispecific T‐cell engaging exosomes, BiTExos). We demonstrated that αPD‐L1×αCD3 BiTExos actively redirected T cells to bind to PD‐L1+ tumor cells, promoting T‐cell activation, proliferation and tumoricidal cytokine production. Furthermore, the αPD‐L1×αCD3 BiTExos promoted T‐cell infiltration into the tumor microenvironment to mitigate the tumor burden in vivo. Our study suggested that the LEAP scaffold may serve as a platform for EV surface display and could be applied for a broad range of EV‐based biomedical applications.
Human placental mesenchymal stromal cell‐derived small extracellular vesicles as a treatment for severe COVID‐19: A double‐blind randomized controlled clinical trial
Authors: Zamanian Mohammad Hossein , Norooznezhad Amir Hossein , Hosseinkhani Zohreh , Hassaninia Daryoush , Mansouri Feizollah , Vaziri Siavash , Payandeh Mehrdad , Heydarpour Fatemeh , Kiani Sara , Shirvani Maria , Rajati Mojgan , Bakhtiari Mitra , Esmaili Farzaneh , Yarani Reza , Mansouri Kamran 
Published: July 2024

AbstractThe current study aimed to investigate the effects of human placental mesenchymal stromal cell‐derived small extracellular vesicles (hPMSC‐sEVs) as a treatment for COVID‐19. This double‐blind, randomized, controlled clinical trial was conducted on two groups of patients with COVID‐19‐associated acute respiratory distress syndrome. After randomization, the control group received standard treatment and placebo, and the intervention arm received standard treatment plus hPMSC‐sEVs. The number of hospital deaths was considered the primary outcome. After meeting the exclusion and inclusion criteria, 21 and 24 patients were allocated to intervention and control arms, respectively. Besides admission SpO2 levels, which were significantly lower in the intervention arm (p = 0.008), all the baseline demo‐biographic and laboratory variables were similar between the groups. It was shown that hPMSC‐sEVs could significantly (p = 0.015) decrease the mortality ratio in the intervention group (4/21 [19.04%]) compared to the controls (13/24 [54.16%]). The mean time to death in the intervention and control groups was 28.06 and 11.10 days, respectively (p < 0.001). This study showed that hPMSC‐sEVs are a possible treatment for critically ill patients with COVID‐19.