bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics of Two Episodes of High Winds Produced by an Unusually Long-Lived Quasi-Linear Convective System in South China
Authors: Xu Xin , Ju Yuanyuan , Liu Qiqing , Zhao Kun , Xue Ming , Zhang Shushi , Zhou Ang , Wang Yuan , Tang Ying 
Published: July 2024

Abstract
Using radar observation and convection-permitting simulation, this work studies the storm-scale dynamics governing the generation of two episodes of high winds by an unusually long-lived quasi-linear convective system (QLCS) in South China on 21 April 2017. The first episode of high winds occurred at the apex of a bowing segment in the southern QLCS due to the downward transport of high momentum by a descending rear-inflow jet (RIJ). The RIJ was initially elevated which was generated as low-frequency gravity wave response to the thermal forcing in the QLCS leading convective line. It descended to the surface owing to the enhancement of low-level diabatic cooling which strengthened the downdrafts at the RIJ leading edge. The vertical momentum budget revealed that the downdrafts were initiated by the negative buoyancy of the cold pool and strengthened by the weakened buoyancy-induced upward pressure gradient force in the boundary layer and enhanced hydrometeor loading above. The second episode of high winds occurred in the decaying stage of the QLCS which, however, redeveloped as its northern part interacted with an intensifying large-scale shear line to the east. A zonal convective line developed along the shear line and finally merged with the QLCS. The merger greatly enhanced the low-level convergence, leading to the downward development of the line-end vortex via vertical stretching of vertical vorticity. The area of high winds was notably increased by the superposition of the ambient translational wind with the vortex rotational flow. The findings provide new insights into the generation of high winds by the QLCS-MCS merger, highlighting the importance of low-level vortices in addition to the RIJ.

Significance Statement
Quasi-linear convective systems (QLCSs) are prolific producers of high winds at the surface. While conceptual models have been established for high winds produced by single QLCSs, this is not the case for the high winds generated by QLCSs merging with other convective systems. This work revealed different dynamics for two episodes of high winds produced by a long-lived QLCS merging with an MCS in South China. The first episode resulted from the descending rear-inflow jet (RIJ) as in the case of single QLCSs. The second and stronger one was due to the superposition of ambient flow with a line-end vortex that developed downward given the enhanced low-level convergence by the merger. This finding sheds light on the importance of mergers in high-wind generation which has even greater damaging potential than the RIJ.
Effects of Turbulence on Upper-Tropospheric Ice Supersaturation
Authors: Kärcher B. , Hoffmann F. , Podglajen A. , Hertzog A. , Pluogonven R. , Atlas R. , Corcos M. , Grabowski W. W. , Gasparini B. 
Published: July 2024

Abstract
Effects of turbulence on ice supersaturation at cirrus heights (>8 km) remain unexplored. Small-scale mixing processes become important for high Reynolds number flows, which may develop below the buoyancy length scale (10–100 m). The current study couples a stochastic turbulent mixing model with reduced dimensionality to an entraining parcel model to investigate, in large-ensemble simulations, how supersaturation evolves due to homogeneous turbulence in the stably stratified, cloud-free upper troposphere. The rising parcel is forced by a mesoscale updraft. The perturbation of an initially homogeneous vertical distribution of supersaturation is studied after a 36-m ascent in a baseline case and several sensitivity scenarios. Turbulent mixing and associated temperature fluctuations alone lead to changes in ensemble-mean distributions with standard deviations in the range 0.001–0.006, while mean values are hardly affected. Large case-to-case variability in the supersaturation field is predicted with fluctuation amplitudes of up to 0.03, although such large values are rare. A vertical gradient of supersaturation (≈10−3 m−1) is generated for high turbulence intensities due to the development of a dry-adiabatic lapse rate. Entrainment of slightly warmer (less than 0.1 K) environmental air into the parcel decreases the mean supersaturation by less than 0.01. Supersaturation fluctuations are substantially larger after entrainment events with an additional small offset in absolute humidity (by ±3.5%) between the parcel and environmental air. The predicted perturbations of ice supersaturation are significant enough to motivate studies of turbulence–ice nucleation interactions during cirrus formation that abandon the assumption of instantaneous mixing inherent to traditional parcel models.

Significance Statement
The purpose of our study is to investigate the effects of microscale turbulence on ice supersaturation in the upper troposphere. The associated variability in temperature and moisture fields is not resolved in cloud models and cannot easily be represented in terms of large-scale flow variables. We specify the conditions in which turbulent mixing and entrainment cause substantial variations in distributions of supersaturation. These include high turbulence intensity, strong atmospheric stability, and large moisture gradients. Our results suggest that turbulence may affect the strongly supersaturation-dependent ice formation processes in high-altitude clouds, pointing to the need to investigate cirrus formation in the presence of turbulence.
Use of HSC-targeted LNP to generate a mouse model of lethal α-thalassemia and treatment via lentiviral gene therapy
Authors: Chappell Maxwell E. , Breda Laura , Tricoli Lucas , Guerra Amaliris , Jarocha Danuta , Castruccio Castracani Carlo , Papp Tyler E. , Tanaka Naoto , Hamilton Nolan , Triebwasser Michael P. , Ghiaccio Valentina , Fedorky Megan T. , Gollomp Kandace L. , Bochenek Veronica , Roche Aoife M. , Everett John K. , Cook Emma J. , Bushman Frederic D. , Teawtrakul Nattiya , Glentis Stavros , Kattamis Antonis , Mui Barbara L. , Tam Ying K. , Weissman Drew , Abdulmalik Osheiza , Parhiz Hamideh , Rivella Stefano 
Published: July 2024

Abstract
α-Thalassemia (AT) is one of the most commonly occurring inherited hematological diseases. However, few treatments are available, and allogeneic bone marrow transplantation is the only available therapeutic option for patients with severe AT. Research into AT has remained limited because of a lack of adult mouse models, with severe AT typically resulting in in utero lethality. By using a lipid nanoparticle (LNP) targeting the receptor CD117 and delivering a Cre messenger RNA (mRNACreLNPCD117), we were able to delete floxed α-globin genes at high efficiency in hematopoietic stem cells (HSC) ex vivo. These cells were then engrafted in the absence or presence of a novel α-globin–expressing lentiviral vector (ALS20αI). Myeloablated mice infused with mRNACreLNPCD117–treated HSC showed a complete knock out (KO) of α-globin genes. They showed a phenotype characterized by the synthesis of hemoglobin H (HbH; also known as β-tetramers or β4), aberrant erythropoiesis, and abnormal organ morphology, culminating in lethality ∼8 weeks after engraftment. Mice infused with mRNACreLNPCD117–treated HSC with at least 1 copy of ALS20αI survived long term with normalization of erythropoiesis, decreased production of HbH, and amelioration of the abnormal organ morphology. Furthermore, we tested ALS20αI in erythroid progenitors derived from α-globin–KO CD34+ cells and cells isolated from patients with both deletional and nondeletional HbH disease, demonstrating improvement in α-globin/β-globin mRNA ratio and reduction in the formation of HbH by high-performance liquid chromatography. Our results demonstrate the broad applicability of LNP for disease modeling, characterization of a novel mouse model of severe AT, and the efficacy of ALS20αI for treating AT.
fENko-Kae01 is a flagellum-specific jumbo phage infecting Klebsiella aerogenes
Authors: Ranta Kira , Skurnik Mikael , Kiljunen Saija 
Published: July 2024

Abstract
Background
Klebsiella aerogenes is an opportunistic pathogen that causes a wide variety of infections. Due to the rising problem of antibiotic resistance, novel antibiotics and strategies to combat bacterial infections are needed. Host-specific bacteriophages are natural enemies of bacteria and can be used in phage therapy as an alternative form of treatment against bacterial infections. Jumbo phages are defined as phages with genomes larger than 200 kb. Relatively few studies have been done on jumbo phages compared to smaller phages.

Results
A novel phage, fENko-Kae01, was isolated from a commercial phage cocktail. Genomic analysis revealed that fENko-Kae01 is a lytic jumbo phage with a 360 kb genome encoding 578 predicted genes. No highly similar phage genomes were identified and fENko-Kae01 may be a completely new genus representative. No known genes associated with lysogenic life cycle, bacterial virulence, or antibiotic resistance were identified. The phage had myovirus morphology and a narrow host range. Phage resistant bacterial mutants emerged under phage selection. Whole genome sequencing revealed that the biogenesis of the flagellum was affected in four mutants and the lack of functional flagellum was confirmed in motility assays. Furthermore, phage fENKo-Kae01 failed to adsorb on the non-motile mutants indicating that the bacterial flagellum is the phage-binding receptor.

Conclusions
fENko-Kae01 is a novel jumbo bacteriophage that is considered safe for phage therapy. fENko-Kae01 uses the flagellum as the phage-binding receptor and may represent a completely novel genus.
High resolution mapping of a novel non-transgressive hybrid susceptibility locus in barley exploited by P. teres f. maculata
Authors: Clare Shaun J. , Alhashel Abdullah F. , Li Mengyuan , Effertz Karl M. , Poudel Roshan Sharma , Zhang Jianwei , Brueggeman Robert S. 
Published: July 2024

AbstractHybrid genotypes can provide significant yield gains over conventional inbred varieties due to heterosis or hybrid vigor. However, hybrids can also display unintended negative attributes or phenotypes such as extreme pathogen susceptibility. The necrotrophic pathogen Pyrenophora teres f. maculata (Ptm) causes spot form net blotch, which has caused significant yield losses to barley worldwide. Here, we report on a non-transgressive hybrid susceptibility locus in barley identified between the three parental lines CI5791, Tifang and Golden Promise that are resistant to Ptm isolate 13IM.3. However, F2 progeny from CI5791 × Tifang and CI5791 × Golden Promise crosses exhibited extreme susceptibility. The susceptible phenotype segregated in a ratio of 1 resistant:1 susceptible representing a genetic segregation ratio of 1 parental (res):2 heterozygous (sus):1 parental (res) suggesting a single hybrid susceptibility locus. Genetic mapping using a total of 715 CI5791 × Tifang F2 individuals (1430 recombinant gametes) and 149 targeted SNPs delimited the hybrid susceptibility locus designated Susceptibility to Pyrenophora teres 2 (Spt2) to an ~ 198 kb region on chromosome 5H of the Morex V3 reference assembly. This single locus was independently mapped with 83 CI5791 × Golden Promise F2 individuals (166 recombinant gametes) and 180 genome wide SNPs that colocalized to the same Spt2 locus. The CI5791 genome was sequenced using PacBio Continuous Long Read technology and comparative analysis between CI5791 and the publicly available Golden Promise genome assembly determined that the delimited region contained a single high confidence Spt2 candidate gene predicted to encode a pentatricopeptide repeat-containing protein.
Application of slow-controlled release fertilizer coordinates the carbon flow in carbon-nitrogen metabolism to effect rice quality
Authors: Jiang Zhengrong , Chen Qiuli , Liu Dun , Tao Weike , Gao Shen , Li Jiaqi , Lin Chunhao , Zhu Meichen , Ding Yanfeng , Li Weiwei , Li Ganghua , Sakr Soulaiman , Xue Lihong 
Published: July 2024

AbstractSlow-controlled release fertilizers are experiencing a popularity in rice cultivation due to their effectiveness in yield and quality with low environmental costs. However, the underlying mechanism by which these fertilizers regulate grain quality remains inadequately understood. This study investigated the effects of five fertilizer management practices on rice yield and quality in a two-year field experiment: CK, conventional fertilization, and four applications of slow-controlled release fertilizer (UF, urea formaldehyde; SCU, sulfur-coated urea; PCU, polymer-coated urea; BBF, controlled-release bulk blending fertilizer). In 2020 and 2021, the yields of UF and SCU groups showed significant decreases when compared to conventional fertilization, accompanied by a decline in nutritional quality. Additionally, PCU group exhibited poorer cooking and eating qualities. However, BBF group achieved increases in both yield (10.8 t hm−2 and 11.0 t hm−2) and grain quality reaching the level of CK group. The adequate nitrogen supply in PCU group during the grain-filling stage led to a greater capacity for the accumulation of proteins and amino acids in the PCU group compared to starch accumulation. Intriguingly, BBF group showed better carbon–nitrogen metabolism than that of PCU group. The optimal nitrogen supply present in BBF group suitable boosted the synthesis of amino acids involved in the glycolysis/ tricarboxylic acid cycle, thereby effectively coordinating carbon–nitrogen metabolism. The application of the new slow-controlled release fertilizer, BBF, is advantageous in regulating the carbon flow in the carbon–nitrogen metabolism to enhance rice quality.
Safety of propofol-assisted deep extubation in the dental treatment of children—a retrospective, observational study
Authors: Zhang Xiang , Wang Xiao-Dong , Cui Wei , Gao Shun-Cai , Yang Xu-Dong , Xia Bin 
Published: July 2024

Abstract
Purpose
Awake extubation and deep extubation are commonly used anesthesia techniques. In this study, the safety of propofol-assisted deep extubation in the dental treatment of children was assessed.

Materials and methods
Children with severe caries who received dental treatment under general anesthesia and deep extubation between January 2017 and June 2023 were included in this study. Data were collected on the following variables: details and time of anesthesia, perioperative vital signs, and incidence of postoperative complications. The incidence of laryngeal spasm (LS) was considered to be the primary observation indicator.

Results
The perioperative data obtained from 195 children undergoing dental treatment was reviewed. The median age was 4.2 years (range: 2.3 to 9.6 years), and the average duration of anesthesia was 2.56 h (range 1 to 4.5 h). During intubation with a videoscope, purulent mucus was found in the pharyngeal cavity of seven children (3.6%); LS occurred in five of them (2.6%), and one child developed a fever (T = 37.8 °C) after discharge. Five children (2.6%) experienced emergence agitation (EA) in the recovery room. Also, 13 children (6.7%) experienced epistaxis; 10 had a mild experience and three had a moderate experience. No cases of airway obstruction (AO) and hypoxemia were recorded. The time to open eyes (TOE) was 16.3 ± 7.2 min. The incidence rate of complications was 23/195 (11.8%). Emergency tracheal reintubation was not required. Patients with mild upper respiratory tract infections showed a significantly higher incidence of complications (P < 0.001).

Conclusions
Propofol-assisted deep extubation is a suitable technique that can be used for pediatric patients who exhibited non-cooperation in the outpatient setting. Epistaxis represents the most frequently encountered complication. Preoperative upper respiratory tract infection significantly increases the risk of complications. The occurrence of EA was notably lower than reported in other studies.
“How difficult it is to change dietary behaviour” experience of older people with sarcopenic obesity: a qualitative study
Authors: Yin Yue-Heng , Liu Justina Yat Wa , Välimäki Maritta 
Published: July 2024

Abstract
Background
Dietary intervention is an important method to manage sarcopenic obesity, but the implementation in real world is difficult to achieve an ideal condition. This study aimed to the experiences of older people with sarcopenic obesity during the implementation of dietary behavioural change (DBC) intervention.

Methods
This study is a semi-structured individual interview embedded within a pilot randomized controlled trial on community-dwelling older people with sarcopenic obesity. Purposive sampling was applied to invite 21 participants who had received a 15-week DBC intervention. The interviews were audio-recorded and transcribed verbatim. Content analysis was performed to analyze the data.

Results
The themes for facilitators included: (a) Attach importance to self’s health; (b) Family’s support; (c) Concern self’s body shape; (d) Instructor’s support; (e) Regular food diary taken. The themes for barriers included: (a) Difficulties of taking food diary; (b) Difficulties of calculating the food amount; (c) Yield to offspring’s appetite; (d) Misjudging self’s or family’s appetite.

Conclusion
Support from family members and instructor, caring about self’s health and body image facilitated the intervention implementation. The complication of food amount estimation and diary record, personal sacrifice for next generations, and previous living experience were barriers for implementing the intervention. Overall, the older people with sarcopenic obesity can accept the design of DBC intervention program and have great willing to join.
Comparison between MRI-negative and positive results and the predictors for a poor prognosis in patients with idiopathic acute transverse myelitis
Authors: Zhou Yu , Chen Qianxi , Gan Weiming , Lin Xiuwen , Wang Bo , Zhou Meihong , Wu Xiaomu , Hong Daojun , Chen Hao 
Published: July 2024

Abstract
Background
Idiopathic acute transverse myelitis (IATM) is a focal inflammatory disorder of the spinal cord that results in motor, sensory, and autonomic dysfunction. However, the comparative analysis of MRI-negative and MRI-positive in IATM patients were rarely reported.

Objectives
The purpose of this study was to compare MRI-negative with MRI-positive groups in IATM patients, analyze the predictors for a poor prognosis, thus explore the relationship between MRI-negative and prognosis.

Methods
We selected 132 patients with first-attack IATM at the First Affiliated Hospital of Nanchang University from May 2018 to May 2022. Patients were divided into MRI-positive and MRI-negative group according to whether there were responsible spinal MRI lesions, and good prognosis and poor prognosis based on whether the EDSS score ≥ 4 at follow-up. The predictive factors of poor prognosis in IATM patients was analyzed by logistic regression models.

Results
Of the 132 patients, 107 first-attack patients who fulfilled the criteria for IATM were included in the study. We showed that 43 (40%) patients had a negative spinal cord MRI, while 27 (25%) patients were identified as having a poor prognosis (EDSS score at follow-up ≥ 4). Compared with MRI-negative patients, the MRI-positive group was more likely to have back/neck pain, spinal cord shock and poor prognosis, and the EDSS score at follow-up was higher. We also identified three risk factors for a poor outcome: absence of second-line therapies, high EDSS score at nadir and a positive MRI result.

Conclusions
Compared with MRI-negative group, MRI-positive patients were more likely to have back/neck pain, spinal cord shock and poor prognosis, with a higher EDSS score at follow-up. The absence of second-line therapies, high EDSS score at nadir, and a positive MRI were risk factors for poor outcomes in patients with first-attack IATM. MRI-negative patients may have better prognosis, an active second-line immunotherapy for IATM patients may improve clinical outcome.
Short stature and vaginal dinoprostone as independent predictors of composite maternal-newborn adverse outcomes in induction of labor after one previous cesarean: a retrospective cohort study
Authors: Tan Sze Ping , Bashirudin Saniyati Badri , Rajaratnam Rajeev Kumar , Gan Farah 
Published: July 2024

Abstract
Background
The rates of labor induction and cesarean delivery is rising worldwide. With the confluence of these trends, the labor induction rate in trials of labor after cesarean can be as high as 27-32.7%. Induction of labor after one previous cesarean (IOLAC) is a high-risk procedure mainly due to the higher risk of uterine rupture. Nevertheless, the American College of Obstetricians and Gynecologists considers IOLAC as an option in motivated and informed women in the appropriate care setting. We sought to identify predictors of a composite of maternal and newborn adverse outcomes following IOLAC.

Methods
The electronic medical records of women who delivered between January 2018 to September 2022 in a Malaysian university hospital were screened to identify cases of IOLAC. A case is classified as a composite adverse outcome if at least one of these 11 adverse outcomes of delivery blood loss ≥ 1000 ml, uterine scar complications, cord prolapse or presentation, placenta abruption, maternal fever (≥ 38 0C), chorioamnionitis, intensive care unit (ICU) admission, Apgar score < 7 at 5 min, umbilical artery cord artery blood pH < 7.1 or base excess ≤-12 mmol/l, and neonatal ICU admission was present. An unplanned cesarean delivery was not considered an adverse outcome as the practical management alternative for a clinically indicated IOLAC was a planned cesarean. Bivariate analysis of participants’ characteristics was performed to identify predictors of their association with composite adverse outcome. Characteristics with crude p < 0.10 on bivariate analysis were incorporated into a multivariable binary logistic regression analysis model.

Results
Electronic medical records of 19,064 women were screened. 819 IOLAC cases and 98 cases with composite adverse outcomes were identified. Maternal height, ethnicity, previous vaginal delivery, indication of previous cesarean, indication for IOLAC, and method of IOLAC had p < 0.10 on bivariate analysis and were incorporated into a multivariable binary logistic regression analysis. After adjustment, only maternal height and IOLAC by vaginal dinoprostone compared to Foley balloon remained significant at p < 0.05. Post hoc adjusted analysis that included all unplanned cesarean as an added qualifier for composite adverse outcome showed higher body mass index, short stature (< 157 cm), not of Chinese ethnicity, no prior vaginal delivery, prior cesarean indicated by labor dystocia, and less favorable Bishop score (< 6) were independent predictors of the expanded composite adverse outcome.

Conclusion
Shorter women and IOLAC by vaginal dinoprostone compared to Foley balloon were independently predictive of composite of adverse outcome.