bluuebunny/crossref_metadata_2025_split huggingface dataset:
Distinct Amino Acids in the C-Linker Domain of the Arabidopsis K+ Channel KAT2 Determine Its Subcellular Localization and Activity at the Plasma Membrane
Authors: Nieves-Cordones Manuel , Chavanieu Alain , Jeanguenin Linda , Alcon Carine , Szponarski Wojciech , Estaran Sebastien , Chérel Isabelle , Zimmermann Sabine , Sentenac Hervé , Gaillard Isabelle 
Published: January 2014

AbstractShaker K+ channels form the major K+ conductance of the plasma membrane in plants. They are composed of four subunits arranged around a central ion-conducting pore. The intracellular carboxy-terminal region of each subunit contains several regulatory elements, including a C-linker region and a cyclic nucleotide-binding domain (CNBD). The C-linker is the first domain present downstream of the sixth transmembrane segment and connects the CNBD to the transmembrane core. With the aim of identifying the role of the C-linker in the Shaker channel properties, we performed subdomain swapping between the C-linker of two Arabidopsis (Arabidopsis thaliana) Shaker subunits, K+ channel in Arabidopsis thaliana2 (KAT2) and Arabidopsis thaliana K+ rectifying channel1 (AtKC1). These two subunits contribute to K+ transport in planta by forming heteromeric channels with other Shaker subunits. However, they display contrasting behavior when expressed in tobacco mesophyll protoplasts: KAT2 forms homotetrameric channels active at the plasma membrane, whereas AtKC1 is retained in the endoplasmic reticulum when expressed alone. The resulting chimeric/mutated constructs were analyzed for subcellular localization and functionally characterized. We identified two contiguous amino acids, valine-381 and serine-382, located in the C-linker carboxy-terminal end, which prevent KAT2 surface expression when mutated into the equivalent residues from AtKC1. Moreover, we demonstrated that the nine-amino acid stretch 312TVRAASEFA320 that composes the first C-linker α-helix located just below the pore is a crucial determinant of KAT2 channel activity. A KAT2 C-linker/CNBD three-dimensional model, based on animal HCN (for Hyperpolarization-activated, cyclic nucleotide-gated K+) channels as structure templates, has been built and used to discuss the role of the C-linker in plant Shaker inward channel structure and function.
A Novel Rice Cytochrome P450 Gene, CYP72A31, Confers Tolerance to Acetolactate Synthase-Inhibiting Herbicides in Rice and Arabidopsis
Authors: Saika Hiroaki , Horita Junko , Taguchi-Shiobara Fumio , Nonaka Satoko , Nishizawa-Yokoi Ayako , Iwakami Satoshi , Hori Kiyosumi , Matsumoto Takashi , Tanaka Tsuyoshi , Itoh Takeshi , Yano Masahiro , Kaku Koichiro , Shimizu Tsutomu , Toki Seiichi 
Published: January 2014

Abstract
Target-site and non-target-site herbicide tolerance are caused by the prevention of herbicide binding to the target enzyme and the reduction to a nonlethal dose of herbicide reaching the target enzyme, respectively. There is little information on the molecular mechanisms involved in non-target-site herbicide tolerance, although it poses the greater threat in the evolution of herbicide-resistant weeds and could potentially be useful for the production of herbicide-tolerant crops because it is often involved in tolerance to multiherbicides. Bispyribac sodium (BS) is an herbicide that inhibits the activity of acetolactate synthase. Rice (Oryza sativa) of the indica variety show BS tolerance, while japonica rice varieties are BS sensitive. Map-based cloning and complementation tests revealed that a novel cytochrome P450 monooxygenase, CYP72A31, is involved in BS tolerance. Interestingly, BS tolerance was correlated with CYP72A31 messenger RNA levels in transgenic plants of rice and Arabidopsis (Arabidopsis thaliana). Moreover, Arabidopsis overexpressing CYP72A31 showed tolerance to bensulfuron-methyl (BSM), which belongs to a different class of acetolactate synthase-inhibiting herbicides, suggesting that CYP72A31 can metabolize BS and BSM to a compound with reduced phytotoxicity. On the other hand, we showed that the cytochrome P450 monooxygenase CYP81A6, which has been reported to confer BSM tolerance, is barely involved, if at all, in BS tolerance, suggesting that the CYP72A31 enzyme has different herbicide specificities compared with CYP81A6. Thus, the CYP72A31 gene is a potentially useful genetic resource in the fields of weed control, herbicide development, and molecular breeding in a broad range of crop species.
The Evolutionary Conserved Oil Body Associated Protein OBAP1 Participates in the Regulation of Oil Body Size
Authors: López-Ribera Ignacio , La Paz José Luis , Repiso Carlos , García Nora , Miquel Mercè , Hernández María Luisa , Martínez-Rivas José Manuel , Vicient Carlos M. 
Published: January 2014

AbstractA transcriptomic approach has been used to identify genes predominantly expressed in maize (Zea mays) scutellum during maturation. One of the identified genes is oil body associated protein1 (obap1), which is transcribed during seed maturation predominantly in the scutellum, and its expression decreases rapidly after germination. Proteins similar to OBAP1 are present in all plants, including primitive plants and mosses, and in some fungi and bacteria. In plants, obap genes are divided in two subfamilies. Arabidopsis (Arabidopsis thaliana) genome contains five genes coding for OBAP proteins. Arabidopsis OBAP1a protein is accumulated during seed maturation and disappears after germination. Agroinfiltration of tobacco (Nicotiana benthamiana) epidermal leaf cells with fusions of OBAP1 to yellow fluorescent protein and immunogold labeling of embryo transmission electron microscopy sections showed that OBAP1 protein is mainly localized in the surface of the oil bodies. OBAP1 protein was detected in the oil body cellular fraction of Arabidopsis embryos. Deletion analyses demonstrate that the most hydrophilic part of the protein is responsible for the oil body localization, which suggests an indirect interaction of OBAP1 with other proteins in the oil body surface. An Arabidopsis mutant with a transfer DNA inserted in the second exon of the obap1a gene and an RNA interference line against the same gene showed a decrease in the germination rate, a decrease in seed oil content, and changes in fatty acid composition, and their embryos have few, big, and irregular oil bodies compared with the wild type. Taken together, our findings suggest that OBAP1 protein is involved in the stability of oil bodies.
(Re)constructing economic citizenship in a welfare state – intersections of gender and class
Authors: Hirsto Heidi , Katila Saija , Moisander Johanna 
Published: January 2014

Purpose
– The purpose of the paper is to discuss and illustrate how contemporary market discourses rearticulate socio-political relationships and identities, including the rights, duties, and opportunities of individuals and categories of individuals as citizens. More specifically, the purpose is to analyze how “economic citizenship” is articulated and negotiated in the intersection of (Nordic) welfare state ideals and shareholder-oriented market discourses. The paper further elaborates on how different identity markers, especially gender and class, intersect in these articulations and contribute to exclusionary practices.

Design/methodology/approach
– The paper approaches the articulation of economic citizenship through an empirical study that focusses on business media representations and online discussions of a major factory shutdown in Finland. Drawing from discourse theory and the notions of representational intersectionality and translocational positionality, the paper analyzes how gender and class intersect in the construction of economic citizenship in the business media.

Findings
– The study illustrates how financialist market discourses render citizenship intelligible in exceedingly economic terms, overriding social and political dimensions of citizenship. The business media construct hierarchies of economic citizens where two categories of actors claim full economic citizenship: the transnational corporation and the transnational investor. Within these categories, particular systems of privilege intersect in similar ways, rendering them masculine and upper middleclass. Whether interpreted as hegemonic or counter-hegemonic, the financialist discourses rearticulate the social hierarchies and moral landscape in Finnish society.

Originality/value
– The paper contributes to critical/feminist management studies by elaborating on the role of the business media as an important site of political identity work, positioning, and moral regulation, where neoliberal ideas, based upon and reproducing masculine and elitist systems of privilege, appear as normalized and self-evidently valued.
Learning organization and innovative behavior
Authors: Kyoung Park Yu , Hoon Song Ji , Won Yoon Seung , Kim Jungwoo 
Published: January 2014

Purpose– The purpose of this study is to investigate the mediating effect of work engagement on the relationship between learning organization and innovative behavior.Design/methodology/approach– This study used surveys as a data collection tool and implemented structural equation modeling for empirically testing the proposed research model.Findings– The study found that learning organization culture makes a direct and indirect impact on employees' innovative work behaviors. Results from hierarchical multiple regressions and structural equation modeling supported that work engagement fully mediates the relationship between the learning organization and innovative work behaviors.Practical implications– HRD practitioners can develop effective interventions to enhance their employees' innovative behavior by devoting efforts to create a workplace that promotes collaborative learning culture and work engagement.Originality/value– This study is valuable to HRD specialists interested in developing effective interventions that encourage employees to engage in innovative behavior.
Leadership's influence on innovation and sustainability
Authors: M. Waite Alina 
Published: January 2014

Purpose– The aim of this study is to review published articles within the HRD and related fields to identify relationships between disparate streams of research (leadership and innovation and sustainability).Design/methodology/approach– Academic research supports the complex relationships between leadership and innovation and leadership and sustainability. An integrative literature review of published articles is used to gain an understanding about effective leadership as an important link between innovation and sustainability.Findings– The analysis revealed the parallel research streams are rather isolated from one another. Central themes focused around leadership roles, orientations, practices, and influences; leadership capacity building; and (global) leadership development. Emergent themes with respect to sustainability included global mindset and concern for others. Servant leadership was brought forth.Research limitations/implications– The literature review was drawn from the four Academy of Human Resource Development (AHRD) journals, as well as sources from the human resource management (HRM), business and management, and related fields.Practical implications– The study discussed influences of leadership on innovation and sustainability, mindsets and competencies, and leadership development strategies for use in understanding how to foster innovation and sustainable practices. Developmental activities including experiential learning, action learning, and service learning programs were considered.Originality/value– This article is among the first to highlight leadership as a connection between innovation and sustainability and provides a valuable platform for HRD scholars and practitioners interested in enhancing leadership capacity and development in these areas.
Innovation and human resource development (HRD)
Authors: Sheehan Maura , N. Garavan Thomas , Carbery Ronan 
Published: January 2014

Purpose– The purpose of this paper is to provide a prologue to the issue of human resource development (HRD) and innovation which has been under-researched despite the widespread recognition of the critical role of innovation for sustained national, regional and organisational competitiveness.Design/methodology/approach– The paper provides an overview of the evolution of innovation within the economic and management literatures. It then examines previous work on human resource management (HRM), HRD and innovation. The paper then provides a brief overview of the articles for this issue focussing on HRD and innovation.Findings– HRD interventions can positively contribute to organisation's innovation activities. HRD is also found to positively influence employee engagement, leadership, manager's motivation to learn, the promotion of a learning culture, and social capital development all of which are positively associated with innovation. Informal HRD and access to mentors and coaches is shown to be critical for innovation in SMEs.Originality/value– This paper provides an introduction to this special issue on HRD and innovation. It shows that this has been an under-researched area and it also emphasises that HRD and its relationship with innovation must be analysed within the context of the organisation's culture, its context, and its leadership capabilities. The four conceptual contributions provide a rich road map for future research on HRD and innovation.
Work-related suicide
Authors: Germain Marie-Line 
Published: January 2014

Purpose
– For the past 50 years, the research literature has shown that employment can contribute to an individual's personal development. Yet, it has also shown that it can become a life-threatening stressor. Reported occupational suicides increased by 22.2 percent between 1995 and 2010, becoming a leading cause of death in the USA. The purpose of this paper is to present the results of six US government reports on employee suicides between 1995 and 2012.

Design/methodology/approach
– Through an interpretive case study approach (Yin, 2003), this study undertook a document analysis of key US government reports examining occupational suicides. Specifically, an analysis of three US Bureau of Labor Statistics reports was undertaken along with other documents, identifying key themes and facts.

Findings
– The analysis of the US government reports reveals a dim legal recognition of employee suicide as an occupational accident. The paper presents the characteristics of suicides as an occupational accident as well as the profile of a typical US occupational suicide victim. Finally, the paper discusses the main causes of employee suicide.

Practical implications
– Organizations have a “duty of care” to their employees, both physical and psychological. Human resource (HR) professionals ought to create preventive policies to minimize work-related suicides and have clear crisis management systems in place, should an employee commit suicide or threaten to do so.

Originality/value
– Occupational distress is not typically apparent or obvious and is not the subject of many studies in the field of HRs. Yet, because of its rampant increase in today's organizations, its direct connection with employee suicide and its impact on organizational revenues, psychological distress in the workplace merits closer attention. This paper is unique as it provides insights for HR professionals based on the analysis of US government reports on work-related suicides.
Comparing tasks of 160 occupations across eight European countries
Authors: G. Tijdens Kea , De Ruijter Esther , De Ruijter Judith 
Published: January 2014

Purpose
– Do similar job titles refer to the same work activities, as assumed often, but hardly empirically tested? The purpose of this paper is to analyze the similarity of 160 occupations within and across eight European countries using interrater agreement statistics (rWG).

Design/methodology/approach
– Using a multilingual web survey, experts and jobholders in the eight countries rated the frequency of ten tasks per occupation they had knowledge of (n=4,197 ratings). Three hypotheses are investigated: first, interrater agreements of occupations are similar regardless the country; second, interrater agreements of occupations are similar within countries; and third, experts and jobholders are similar in their ratings.

Findings
– Half of the occupations reveal no agreement across ratings, one-third shows a weak/moderate agreement and one in ten shows a strong agreement. H1 is rejected for task frequency but not for task importance. Within-country similarity of occupations is larger than across-country similarity. H2 is supported for two countries and rejected for two other countries. H3 is not supported. Jobholders demonstrate higher agreement than experts.

Research limitations/implications
– An empirical testing of occupation-specific tasks for a wide range of occupations across Europe seems a viable approach.

Practical implications
– The paper contributes to the challenges related to labor market mobility across borders.

Originality/value
– Work tasks for a wide range of occupations and countries, using job-specific work activities in combination with web surveys and the internet for recruitment of jobholders, have not been used before.
An analysis of copyright protection in Saudi Arabia
Authors: Mastura Muhammad Arif Afida 
Published: January 2014

Purpose– This paper aims to explore the copyright laws of Saudi Arabia, illustrating the provisions governing the law. It seeks to outline Saudi Arabia copyright law within the framework of the international copyright protection, which include the Berne Convention and the TRIPS agreement.Design/methodology/approach– The paper is a thorough scrutinizing of the legal provisions of the Saudi Copyright Laws in regard to protected works, author's exclusive rights, lawful use of copyrighted works and the exceptions, mandatory licenses, duration of protection, provisions of infringements and penalties. The highlighted issue is concerning the exceptions in regard to public interest, particularly relating to education purposes.Findings– The paper finds that the Saudi Arabia Copyright Laws have met the requirement of the international copyright laws. The conditions for mandatory license and the 12 exceptions which permit lawful usage of copyrighted work under the copyright law without seeking the permission of the author are also in line with the international copyright laws, as public interest is the paramount consideration in exercising these exceptions.Originality/value– There is lack of study on copyright law in the Saudi Arabiaper se. Therefore, this study on the Saudi Arabia copyright laws seeks to fill in this gap and to provoke further discussion on this issue. It should be useful to the academic community, particularly in the Saudi Arabia and the Gulf Cooperation Council countries.
Lifting the veil of incorporation under common law and statute
Authors: Nyombi Chrispas 
Published: January 2014

Purpose
– The paper examines case law and statutory provisions related to lifting the corporate veil. The aim of the paper is to explore recent case law in order to determine whether courts have moved away from an overly restrictive approach when dealing with cases relating to the corporate personality. To offer a full account of the exceptions to the corporate personality doctrine, this paper also examines cases where the veil of incorporation is lifted due to a breach of a statutory provision.

Design/methodology/approach
– The paper reviews recent case law and statutory provisions relating to lifting the corporate veil. The paper critically reviews the exceptions to the corporate personality doctrine which amount to lifting the corporate veil.

Findings
– The paper finds that courts are more willing to lift the corporate veil compared to before. They have moved away from the restrictive approach and this is demonstrated by the tendency to find new exceptions to the corporate personality doctrine such as the interests of justice argument or lifting the veil in tort cases.

Originality/value
– The paper offers an up-to-date assessment of the exceptions to the corporate personality doctrine and highlights the growing tendency to finding new ways of lifting the corporate veil.
Tax complexities in the Malaysian corporate tax system: minimise to maximise
Authors: Isa Khadijah 
Published: January 2014

Purpose– This paper aims to examine areas of tax difficulties encountered by corporate taxpayers in complying with tax obligations under the self-assessment system.Design/methodology/approach– A two-phase exploratory mixed methods approach was employed. The first phase involves eight focus group interviews with 60 tax auditors from the Inland Revenue Board of Malaysia (IRBM) and the second phase adopts a mixed-mode survey among selected Malaysian corporate taxpayers. Thematic analysis and descriptive and inferential analysis were used to examine the qualitative and quantitative data in achieving the objective.Findings– Three dimensions of tax complexity encountered by corporate taxpayers were tax computations, record keeping and tax ambiguity. The first two complexity dimensions were faced largely by smaller companies. On the other hand, the least difficult tax-related areas were dealing with tax agents, submitting tax returns within the given time and dealing with the tax authority.Practical implications– In a tax policy context, this study enables international tax authorities in general, and Malaysian tax authority in particular, to have greater confidence in developing and administering tax laws and policies to maintain and/or increase the overall level of corporate tax compliance.Originality/value– Unlike prior studies that mainly used individual taxpayers or students as research participants, this study employed corporate tax auditors from the tax authority and corporate tax officers. Tax auditors and corporate taxpayers provide invaluable insights into the possible determinants of compliance variables. These insights are based on their practical experience in handling corporate tax audits and managing corporate tax matters, respectively.
The evidence of compelling arguments in agenda building
Authors: Rim Hyejoon , Hong Ha Jin , Kiousis Spiro 
Published: January 2014

Purpose– This paper aims to explore the links among health authorities’ public relations efforts, news media coverage, and public perceptions of risk during the H1N1 pandemic outbreak.Design/methodology/approach– This study used a triangulation of research methods by comparing public relations materials, media coverage, and public opinion. The data were collected from a federal government web site, national newspapers, and national polls.Findings– The data revealed a positive relationship between information subsidy attention and media attention to the H1N1 disease as well as the severity attribute. The salience of the severity attribute in information subsidies was linked with increased H1N1 salience in media coverage, extending the testing of the compelling-arguments hypothesis to an agenda-building context. However, there was no association between salience of the severity attribute and public risk perceptions.Research limitations/implications– The study provides evidence for public relations effectiveness. However, the limited influence of the severity frame on the public's risk perception suggests a gap between news coverage and the public's view. Framing that effectively empowers the public to engage in desired behavior should be further studied for the success of a public health campaign. The study is limited to examining the severity attribute. A future study should pay more attention to different issue attributes or other frames. The media sample was limited to newspapers and thus lacks generalizability.Originality/value– The study contributes to public relations scholarship by demonstrating how information subsidies influence media agendas and public opinion in a health communication context. The public health authorities’ role in influencing media agenda should be stressed.