bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation, Characterization, and Differentiation of Progenitor Cells from Human Adult Adrenal Medulla
Authors: Santana Magda M. , Chung Kuei-Fang , Vukicevic Vladimir , Rosmaninho-Salgado Joana , Kanczkowski Waldemar , Cortez Vera , Hackmann Karl , Bastos Carlos A. , Mota Alfredo , Schrock Evelin , Bornstein Stefan R. , Cavadas Cláudia , Ehrhart-Bornstein Monika 
Published: October 2012

AbstractChromaffin cells, sympathetic neurons of the dorsal ganglia, and the intermediate small intensely fluorescent cells derive from a common neural crest progenitor cell. Contrary to the closely related sympathetic nervous system, within the adult adrenal medulla a subpopulation of undifferentiated progenitor cells persists, and recently, we established a method to isolate and differentiate these progenitor cells from adult bovine adrenals. However, no studies have elucidated the existence of adrenal progenitor cells within the human adrenal medulla. Here we describe the isolation, characterization, and differentiation of chromaffin progenitor cells obtained from adult human adrenals. Human chromaffin progenitor cells were cultured in low-attachment conditions for 10–12 days as free-floating spheres in the presence of fibroblast growth factor-2 (FGF-2) and epidermal growth factor. These primary human chromosphere cultures were characterized by the expression of several progenitor markers, including nestin, CD133, Notch1, nerve growth factor receptor, Snai2, Sox9, Sox10, Phox2b, and Ascl1 on the molecular level and of Sox9 on the immunohistochemical level. In opposition, phenylethanolamine N-methyltransferase (PNMT), a marker for differentiated chromaffin cells, significantly decreased after 12 days in culture. Moreover, when plated on poly-l-lysine/laminin-coated slides in the presence of FGF-2, human chromaffin progenitor cells were able to differentiate into two distinct neuron-like cell types, tyrosine hydroxylase (TH)+/β-3-tubulin+ cells and TH−/β-3-tubulin+ cells, and into chromaffin cells (TH+/PNMT+). This study demonstrates the presence of progenitor cells in the human adrenal medulla and reveals their potential use in regenerative medicine, especially in the treatment of neuroendocrine and neurodegenerative diseases.
Coculture-Driven Mesenchymal Stem Cell-Differentiated Articular Chondrocyte-Like Cells Support Neocartilage Development
Authors: Yang Yueh-Hsun , Lee Anna J. , Barabino Gilda A. 
Published: October 2012

Abstract
Controlled differentiation of mesenchymal stem cells (MSCs) into the chondrogenic lineage is crucial for in vitro generation of neocartilage, yet achieving it remains challenging. Traditional protocols for MSC differentiation using exogenous inductive molecules, such as transforming growth factor-β, fall short in meeting the needs of clinical applications because they yield differentiated cells that exhibit hypertrophic characteristics and subsequently facilitate endochondral bone formation. The objective of the current study was to deliver endogenous inductive factors from juvenile articular chondrocytes to bone marrow-derived MSCs to drive MSC chondrogenic differentiation through cocultivation of the two cell types in the absence of direct physical contact and exogenous stimulators. An initial chondrocyte/MSC ratio of 63:1 was identified as the appropriate proportion of the two cell populations to ensure that coculture-driven MSC-differentiated (CDMD) cells replicated the cellular morphology, behavior, and phenotype of articular chondrocytes. In a three-dimensional agarose system, CDMD cells were further shown to develop into robust neocartilage structurally and mechanically stronger than chondrocyte-laden constructs and with reduced hypertrophic potential. Although MSCs tended to lose the ability to express CD44, an important regulator in cartilage biology, during the coculture induction, CDMD cells regained this function in the three-dimensional tissue cultivation. The present work establishes a chondrocyte/MSC coculture model that serves as a template to better understand chondrocyte-driven MSC differentiation and provides insights for improved strategies to develop clinically relevant cartilage tissue replacements.
Penser pour agir : la recherche et les partenariats au service de la dualité linguistique
Authors: Tardif* Claudette 
Published: October 2012

L’auteure soulève quelques pistes de réflexion par rapport à la recher­che relative à la dualité linguistique. Comment peut-on concilier les objectifs de recherche divergents des acteurs gouvernementaux, univer­sitaires et communautaires en matière de dualité linguistique ? Quels ont été les principaux thèmes de recherche relatifs à la dualité linguistique au cours des dernières années ? Comment s’assurer que la recherche entreprise reflète les besoins et les réalités des communautés de langue officielle ? Voilà le type de questions posées dans cet article.
Préface. Enracinement et identités
Authors: Pelletier Benoît 
Published: October 2012

Le thème de l’enracinement est particulièrement évocateur. Il fait allusion aux traces laissées par le passage du temps, à une prise solide sur le réel, à une présence profonde, soutenue et durable, à la longévité, à la fermeté et à la dignité. C’est le contraire de la superficialité, de la vacuité. Cette image convient parfaitement à la francophonie canadienne. Cette dernière n’est-elle pas fermement ancrée, fixée dans l’espace canadien ? Ne fait-elle pas partie depuis longtemps du paysage qui compose le Canada ? N’est-elle pas irriguée par l’histoire ? N’a-t-elle pas surgi d’un sol parfois aride pour se dresser d’un seul corps, croître et s’étendre comme l’arbre qui, de sa seule présence, semble défier son entourage ? Dans sa préface, l’auteur se sert de l’enracinement pour illustrer la constance et la ténacité qui caractérisent la présence française au Canada et dans le reste des Amériques. Renvoyant au passage aux nombreux défis que la mondialisation pose à la langue française et à l’identité francophone sur ce continent, il tisse un lien entre le passé et le présent et termine sur une note limpide, source de sa confiance en un avenir… en français !
L’intégration d’un traitement psychologique en réadaptation cardiaque à l’Hôpital Montfort : une diminution des risques associés aux maladies cardiaques
Authors: Greenman Paul S. , Morin-Turmel Anaïs , Grenier Jean 
Published: October 2012

Les buts de cette étude étaient l’intégration et la mise à l’épreuve d’un traitement psychologique au sein du Service de réadaptation cardiaque à l’Hôpital Montfort. Des patients (hommes et femmes), âgés entre 35 et 85 ans, avec une maladie cardiaque et qui se plaignaient de symptômes d’anxiété et de dépression ont participé à cette étude. Les résultats suggèrent que la thérapie cognitivo-comportementale (TCC) peut réduire de façon significative les symptômes de dépression et d’anxiété chez les patients cardiaques. Dans notre échantillon (N = 11), les patients ayant suivi au moins dix séances de traitement ont présenté une diminution importante de leurs symptômes, et personne ne répondait aux critères d’un diagnostic de dépression ou d’anxiété après l’intervention.
Les descriptions de villages dans l’oeuvre de Gabrielle Roy
Authors: Dolbec Nathalie 
Published: October 2012

De Fragiles lumières de la terre à La détresse et l’enchantement, une étude diachronique du descriptif, examine, sous l’angle narratologique, vingt-cinq descriptions de villages chez Gabrielle Roy. L’analyse de l’implantation du segment descriptif, puis celle des opérations de schématisation, d’aspectualisation et de mise en situation spatiale révèlent un système à fonction signalétique. Le village comme objet décrit suscite une quête – factuelle et allégorique. Dans le cheminement rhétorique conduisant d’Iberville à Encinitas puis à Upshire, ce dernier apparaît comme le village royen par excellence, à la fois ouvert sur le monde et douillettement replié sur lui-même : un nid-belvédère, en somme.
Nancy Huston, empreintes et failles d’une mémoire sans frontières
Authors: Lepage Élise 
Published: October 2012

L’empreinte de l’ange (1998) et Lignes de faille (2006), de Nancy Huston, sont des romans qui traitent de certains grands conflits du siècle passé et dont les narrateurs et les personnages sont d’origines diverses. Cet article étudie comment ces « romans mémoriels » problématisent la pluralité des mémoires et leurs récits croisés, parfois conflictuels. Par son choix d’aborder des tragédies qui ont frappé des peuples très différents, Nancy Huston semble suggérer que chacune n’appartient pas uniquement à une mémoire nationale, mais que toutes relèvent d’une mémoire internationale qu’assume une littérature-monde libérée des frontières et des critères d’appartenance nationale.
L’Acadie de la diversité chez le militant acadien d’ici et l’immigrant francophone venu d’ailleurs : contradictions et convergences dans les représentations d’une identité commune
Authors: Violette Isabelle , Traisnel Christophe 
Published: October 2012

Nous cherchons à voir comment s’articule, dans les discours identitaires en Acadie du Nouveau-Brunswick, l’ouverturede factode la communauté acadienne d’ici à la francophonie venue d’ailleurs : quel impact cette mise en contact produit sur les représentations identitaires des francophones d’ici et d’ailleurs ? Pour ce faire, nous proposons un portrait croisé et une comparaison de deux groupes qui, de par leurs positionnements, peuvent refléter ces changements de représentations au sein de l’Acadie du Nouveau-Brunswick, à savoir les militants acadiens et les immigrants francophones. Malgré des points de tension et de divergence, notamment autour du poids accordé à la dimension ethnique de l’identité acadienne, nous avons constaté un terrain d’entente identitaire entre les deux groupes autour d’unlieud’appartenance commun. En effet, si devenir Acadien demeure problématique pour les immigrants, l’intégration à la communauté d’accueil locale tend à prendre la forme d’une identification à l’Acadie comme espace de vivre ensemble.
Références historiennes : l’historiographie acadienne contemporaine et l’influence québécoise
Authors: Massicotte Julien 
Published: October 2012

Dans cette étude, l’auteur examine la nature des rapports qu’entretiennent les historiens acadiens des dernières décennies avec l’historiographie québécoise, et tente de comprendre l’évolution temporelle de ces rapports. Les rapports entretenus avec l’historiographie québécoise sont examinés à partir de deux cohortes d’historiens, l’une particulièrement active dans les années 1970 et 1980, l’autre dans les années 1980 et 1990. L’auteur tente, dans cet article, d’examiner l’espace que la production historienne et les sources historiques en provenance du Québec occupent au sein des ouvrages des historiens acadiens des dernières décennies, en comparaison avec d’autres sources d’influence possible. Quatorze textes clés issus de l’historiographie acadienne des dernières décennies ont été retenus, afin d’y mesurer le poids et la présence de différentes références savantes, historiennes ou archivistiques.
Les commissions scolaires et les immigrants à Toronto et à Montréal (1900-1945) : quatre modèles d’intégration en milieu urbain
Authors: Croteau Jean-Philippe 
Published: October 2012

À la fin duxixesiècle et au début duxxesiècle, les commissions scolaires montréalaises et torontoises définissent des modèles d’intégration pour assimiler les enfants d’immigrants à la culture, aux valeurs et aux normes de la société d’accueil. Cet article présente successivement les fondements de ces modèles d’intégration, leur mise en oeuvre et les stratégies employées pour favoriser la « canadianisation » des immigrants. En dépit de visées communes, les commissions scolaires ont développé des approches distinctes, qui reflètent les conditions sociales et culturelles de leur milieu, pour assurer l’intégration des immigrants à la société canadienne.
Use of disease‐modifying antirheumatic drugs during pregnancy and risk of preeclampsia
Authors: Palmsten Kristin , Hernández‐Díaz Sonia , Kuriya Bindee , Solomon Daniel H. , Setoguchi Soko 
Published: October 2012

AbstractObjectiveTo describe patterns of disease‐modifying antirheumatic drug (DMARD) use during pregnancy in a population‐based cohort, and to evaluate the association between autoimmune disease, DMARDs, corticosteroids, and nonsteroidal antiinflammatory drugs (NSAIDs) and preeclampsia.MethodsUsing health care utilization databases from British Columbia (1997–2006), we compared the risk for preeclampsia among 44,786 women with and without autoimmune disease with study drug dispensings before pregnancy (past users) and before and during the first 20 gestational weeks (continuous users). Relative risks (RRs) and 95% confidence intervals (95% CIs) were estimated.ResultsOnly 414 women (0.1%) had a DMARD dispensing during pregnancy. The incidence of preeclampsia was 2.3% for past DMARD users, 2.7% for past corticosteroid users, and 2.9% for past NSAID users. Compared to past users, the continuous DMARD user RR was 2.29 (95% CI 0.81–6.44), and was 0.89 (95% CI 0.51–1.56) for corticosteroid and 0.84 (95% CI 0.63–1.10) for NSAID users. Compared to women without autoimmune disease, the delivery year–adjusted RR was 2.02 (95% CI 1.11–3.64) for women with systemic lupus erythematosus (SLE). The DMARD results were attenuated when antimalarials were excluded, and the delivery year–adjusted RR was 0.95 (95% CI 0.25–3.55) when the DMARD analysis was restricted to women with autoimmune disease.ConclusionFew women were exposed to DMARDs during pregnancy. We observed a 2‐fold increased risk of preeclampsia among women with SLE and a nonsignificant increase in risk in DMARD users. The DMARD and preeclampsia association was attenuated when antimalarials were excluded and null when restricted to women with autoimmune disease, which suggests the association is likely due to greater autoimmune disease severity in DMARD users.
Improved Triclosan Delivery by a Novel Silica‐Based Nanocomposite
Authors: Makarovsky Igor , Lellouche Jonathan , Lellouche Jean‐Paul , Banin Ehud 
Published: October 2012

AbstractIn this study, we report on the design, synthesis, and full characterization of a covalently‐linked, triclosan silica‐based nanoparticles (T‐SNPs), coated with a polyaminated shell (NH2‐T‐SNPs). Various techniques are used to elucidate and rationalize the potential biological mechanism of action of these novel nanoparticles. NH2‐T‐SNPs are found to be potently bactericidal with no detectable lag time for the antimicrobial activity against E. coli and S. aureus. In this context, we also prove that triclosan is the chemical agent that mediated the bactericidal activity of these chemically‐modified NPs. The obtained experimental data allows us to pinpoint the actual minimal bactericidal concentrations (MBCs) of triclosan‐bound NPs by quantifying intracellular triclosan concentrations. Furthermore, we conduct preliminary cytotoxicity studies, which show that triclosan bound NPs are less cytotoxic (2000 fold) in vitro compared to free‐triclosan when tested with various human and mammalian cell lines. Taken together, our results further support the characterization and development of these new nanoscale materials for various biomedical applications.
Iron‐Catalyzed Amidation of Aldehydes with N‐Chloroamines
Authors: Porcheddu Andrea , Luca Lidia De 
Published: October 2012

AbstractA new direct conversion of aldehydes to amides has been realized, in the presence of iron(III) chloride as a catalyst and using tert‐butyl hydroperoxide (TBHP) as an oxidant. Both aliphatic and aromatic aldehydes were successfully reacted with variously mono‐ and di‐substituted N‐chloroamines. The methodology has a wide substrate scope, uses cheap and easily available reagents and is characterized by short reaction times.
Precursor Decomposition, Microstructure, and Porosity of Yttria Stabilized Zirconia Thin Films Prepared by Aerosol‐Assisted Chemical Vapor Deposition
Authors: Schlupp Meike V. F. , Martynczuk Julia , Prestat Michel , Gauckler Ludwig J. 
Published: October 2012

AbstractMicrostructures of yttria stabilized zirconia thin films deposited by aerosol assisted chemical vapor deposition (AA‐CVD) are correlated with the thermal decomposition behavior of the corresponding metal precursors, zirconium and yttrium 2,4‐pentanedionate. Process conditions of AA‐CVD are investigated with the aim of producing dense and compact YSZ thin films for applications as gas‐tight electrolyte. Based on systematic cross sectional scanning transmission electron microscopy (STEM) investigations and conductivity measurements, the development of percolating nanoporosity is observed in samples prepared at temperatures between 350 °C and 600 °C at standard solution throughput. Compact columnar thin films with bulk conductivity are obtained at 600 °C by reducing the metal content of the precursor solution and at 450 °C by reducing the solution throughput.
In Situ Formation of MoO3 in PEDOT:PSS Matrix: A Facile Way to Produce a Smooth and Less Hygroscopic Hole Transport Layer for Highly Stable Polymer Bulk Heterojunction Solar Cells
Authors: Shao Shuyan , Liu Jian , Bergqvist Jonas , Shi Shengwei , Veit Clemens , Würfel Uli , Xie Zhiyuan , Zhang Fengling 
Published: October 2012

AbstractA solution‐processed neutral hole transport layer is developed by in situ formation of MoO3 in aqueous PEDOT:PSS dispersion (MoO3‐PEDOT:PSS). This MoO3‐PEDOT:PSS composite film takes advantage of both the highly conductive PEDOT:PSS and the ambient conditions stability of MoO3; consequently it possesses a smooth surface and considerably reduced hygroscopicity. The resulting bulk heterojunction polymer solar cells (BHJ PSC) based on poly[2,3‐bis‐(3‐octyloxyphenyl)quinoxaline‐5,8‐diyl‐alt‐thiophene‐2,5‐diyl] (TQ1):[6,6]‐phenyl‐C71‐butyric acid methyl ester (PC70BM) blends using MoO3‐PEDOT:PSS composite film as hole transport layer (HTL) show considerable improvement in power conversion efficiency (PCE), from 5.5% to 6.4%, compared with the reference pristine PEDOT:PSS‐based device. More importantly, the device with MoO3‐PEDOT:PSS HTL shows considerably improved stability, with the PCE remaining at 80% of its original value when stored in ambient air in the dark for 10 days. In comparison, the reference solar cell with PEDOT:PSS layer shows complete failure within 10 days. This MoO3‐PEDOT:PSS implies the potential for low‐cost roll‐to‐roll fabrication of high‐efficiency polymer solar cells with long‐term stability at ambient conditions.
Prevalence of epicondylitis, rotator cuff syndrome, and low back pain in latino poultry workers and manual laborers
Authors: Rosenbaum Daryl A. , Grzywacz Joseph G. , Chen Haiying , Arcury Thomas A. , Schulz Mark R. , Blocker Jill N. , Mora Dana C. , Quandt Sara A. 
Published: October 2012

AbstractBackgroundThe goal of this study is to improve understanding of immigrant Latino manual workers' occupational health, focusing on upper body musculoskeletal injury.MethodsPhysical exams were conducted with a representative sample of 516 Latino poultry workers and manual laborers in western North Carolina; outcome measures were prevalence of epicondylitis, rotator cuff syndrome, and low back pain.ResultsLow back pain (n = 89; 17.2%) and rotator cuff syndrome (n = 76; 14.7%) indicated by physical exam was common. Epicondylitis was less common, but still frequent (n = 30; 5.8%). Prevalence of each outcome did not differ between poultry processing workers and other manual workers. Workers >40 years old had greater incidence of rotator cuff syndrome and epicondylitis.ConclusionsEpicondylitis, rotator cuff syndrome, and low back pain are common in immigrant Latino workers, and may negatively impact long‐term health and contribute to occupational health disparities. Am. J. Ind. Med. 56:226–234, 2013. © 2011 Wiley Periodicals, Inc.
Dust exposure and the risk of cancer in cement industry workers in Korea
Authors: Koh Dong‐Hee , Kim Tae‐Woo , Jang SeungHee , Ryu Hyang‐Woo 
Published: October 2012

AbstractBackgroundCement is used widely in the construction industry, though it contains hazardous chemicals such as hexavalent chromium. Several epidemiological studies have examined the association between cement dust exposure and cancer, but these associations have proved inconclusive. In the present study, we examined the association between dust exposure and cancer in cement industry workers in Korea.MethodsOur cohort consisted of 1,324 men who worked at two Portland cement manufacturing factories between 1997 and 2005. We calculated cumulative dust exposures, then categorized workers into high and low dust exposure groups. Cancer cases were identified between 1997 and 2005 by linking with the national cancer registry. Standardized incidence ratios (SIRs) were calculated for all workers and the high and low dust exposure groups, respectively.ResultsThe SIR for overall cancers in all workers was increased (1.35, 95% CI: 1.01–1.78). The SIR for stomach cancer in the high dust exposure group was increased (2.18, 95% CI: 1.19–3.65), but there was no increased stomach cancer risk in the low dust exposure group. The SIR for rectal cancer in all workers was increased (3.05, 95% CI: 1.32–6.02). Rectal cancer risk was similar in the high and low exposure groups.ConclusionsOur findings suggest a potential association between exposure in the cement industry and an increased risk of stomach and rectal cancers. However, due to the small number of cases, this association should be further investigated in a study with a longer follow‐up period and adjustment for confounders. Am. J. Ind. Med. 56:276–281, 2013. © 2012 Wiley Periodicals, Inc.
Digestion products of the PH20 hyaluronidase inhibit remyelination
Authors: Preston Marnie , Gong Xi , Su Weiping , Matsumoto Steven G. , Banine Fatima , Winkler Clayton , Foster Scott , Xing Rubing , Struve Jaime , Dean Justin , Baggenstoss Bruce , Weigel Paul H. , Montine Thomas J. , Back Stephen A. , Sherman Larry S. 
Published: October 2012

ObjectiveOligodendrocyte progenitor cells (OPCs) recruited to demyelinating lesions often fail to mature into oligodendrocytes (OLs) that remyelinate spared axons. The glycosaminoglycan hyaluronan (HA) accumulates in demyelinating lesions and has been implicated in the failure of OPC maturation and remyelination. We tested the hypothesis that OPCs in demyelinating lesions express a specific hyaluronidase, and that digestion products of this enzyme inhibit OPC maturation.MethodsMouse OPCs grown in vitro were analyzed for hyaluronidase expression and activity. Gain of function studies were used to define the hyaluronidases that blocked OPC maturation. Mouse and human demyelinating lesions were assessed for hyaluronidase expression. Digestion products from different hyaluronidases and a hyaluronidase inhibitor were tested for their effects on OPC maturation and functional remyelination in vivo.ResultsOPCs demonstrated hyaluronidase activity in vitro and expressed multiple hyaluronidases, including HYAL1, HYAL2, and PH20. HA digestion by PH20 but not other hyaluronidases inhibited OPC maturation into OLs. In contrast, inhibiting HA synthesis did not influence OPC maturation. PH20 expression was elevated in OPCs and reactive astrocytes in both rodent and human demyelinating lesions. HA digestion products generated by the PH20 hyaluronidase but not another hyaluronidase inhibited remyelination following lysolecithin‐induced demyelination. Inhibition of hyaluronidase activity lead to increased OPC maturation and promoted increased conduction velocities through lesions.InterpretationWe determined that PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit OPC maturation and remyelination. Pharmacological inhibition of PH20 may therefore be an effective way to promote remyelination in multiple sclerosis and related conditions. ANN NEUROL 2013;73:266–280