bluuebunny/crossref_metadata_2025_split huggingface dataset:
ST2L promotes VEGFA-mediated angiogenesis in gastric cancer by activating TRAF6/PI3K/Akt/NF-κB pathway via IL-33
Authors: Zhu yanqing , Lu Yuxin , Zhu Yifei , Ren Xiaolu , Deng Qinyi , Yang Muqing , Liang Xin 
Published: July 2024

Abstract

Tumorigenicity 2 (ST2) is a member of the interleukin-1 receptor/ Toll-like receptor superfamily, and its specific ligand is Interleukin-33 (IL-33). IL-33/ ST2 signaling has been implicated in numerous inflammatory and allergic diseases, as well as in promoting malignant behavior of tumor cells and angiogenesis. However, the precise role of ST2 in gastric cancer angiogenesis remains incompletely elucidated. We observed a significant correlation between high expression of ST2 in gastric cancer tissues and poor prognosis, along with various clinicopathological features. In vitro experiments demonstrated that the IL-33/ ST2 axis activates the PI3K/AKT/NF-κB signaling pathway through TRAF6, thereby promoting VEGFA-mediated tumor angiogenesis; meanwhile sST2 acts as a decoy receptor to regulate the IL-33/ST2L axis. Consistent findings were also observed in subcutaneous xenograft tumor models in nude mice. Furthermore, we investigated the molecular mechanism by which IL-33 promotes ST2L expression in GC cells via upregulation of transcription factors YY1 and GATA2 through intracellular signaling pathways.
The Association of Ultrasound Parameters with De Novo Stress Urinary Incontinence following Transvaginal Mesh Surgery
Authors: Chang Chieh-Yu , Loo Zi-Xi , Lin Kun-Ling , Sung I-Chieh , Long Cheng-Yu 
Published: July 2024

Abstract

Pelvic organ prolapse (POP) often coexists with stress urinary incontinence (SUI), posing challenges in surgical management. De novo SUI post-surgery remains unpredictable, prompting this study to explore ultrasound's role in assessing de novo SUI after transvaginal mesh (TVM) surgery. A retrospective analysis of 696 women undergoing TVM surgery revealed a 36.9% incidence of de novo SUI. Specific ultrasound parameters, such as proximal urethral rotational angle and levator urethral gap during straining, were found associated with de novo SUI after TVM surgery. Despite certain limitations in this study, ultrasound emerges as a valuable tool for assessing pelvic floor integrity and guiding clinical decisions in urogynecology.
Interaction between dietary quality and depression on glycaemic control in type 2 diabetics
Authors: Jiang Yixue , Lou Heqing , Xu Chunrong , Dong Zongmei , Yin Zeyuan , Liu De , Dong Dong , Qiao Cheng , Zhang Pan , Chen Fang 
Published: July 2024

Abstract

Background
Poorer diet quality and depression were both independent influences on poor glycaemic control in patients with type 2 diabetes (T2DM). The current study aimed to investigate the interaction between dietary quality and depression on blood sugar control in T2DM patients.
Methods
In the cross-sectional study, Participants were selected using a multistage cluster random method, and the Meal Frequency Questionnaire and the Patient Health Questionnaire-9 were respectively used to investigate 1088 patients' dietary quality and depression. Data of Physical measurement, glycated haemoglobin, and blood lipid detection were also collected.
Results
The rate of poor glycaemic control was 38.3% in patients with T2DM with poor dietary quality. After adjusting for other variables, the risk of poor glycaemic control in patients with T2DM with poor dietary quality and no depression was 2.04 times higher than that in patients with T2DM with good dietary quality and no depression; the risk of poor glycaemic control in patients with T2DM with poor dietary quality and no depression was 1.96 times higher than that in patients with T2DM with good dietary quality and no depression; when poor dietary quality and depression co-existed, patients with T2DM with poor dietary quality and depression were at greater risk for poor glycaemic control.
Conclusion
The study revealed that in individuals with T2DM, there was a significant additive interaction between poor dietary quality and depressive symptoms, exacerbating poor glycaemic control. This synergistic effect suggested that concurrent management of dietary habits and mental health is crucial for optimizing glycaemic outcomes in this population.
Overexpression of EuSIP5 gene to improve drought resistance in tobacco
Authors: Lin YueLing , Chen Xi , Zhao DeGang , Li Chao 
Published: July 2024

Abstract

In order to study the drought resistance of the Eucommia ulmoides soluble inorganic pyrophosphatase 5 (EuSIP5) gene, the cDNA sequence of the EuSIP5 gene was amplified from Eucommia ulmoides Oliver (E. ulmoides) by RT-PCR. Bioinformatic analysis indicated that EuSIP5 might be an unstable amphipathic protein, mainly located in the plasma and nuclear membranes. E. ulmoides, the highest expression of the EuSIP5 gene was found in male leaves and pericarp from April to October, and in leaves of E. ulmoides in July and September. During drought, the expression of EuSIP5 in seedling E. ulmoides leaves was significantly higher than that of the control. An overexpression vector harboring the EuSIP5 was constructed and introduced into the tobacco (Nicotiana tabacum cv. Xanthi). We found that during drought stress, the appearance of wilting was delayed by 4 days in transgenic tobacco plants (OE) compared with wild type plants (WT), and the expression of drought-related genes DET2, CYP85A1, P5CS, ERF1 and F-box was found to be increased in transgenic tobacco leaves, as well as the enzyme activities of the protective enzymes: peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) were significantly higher than those of the WT, and the malondialdehyde (MDA) content was lower than the WT. These results indicated that the introduction of EuSIP5 gene into tobacco increased the expression of drought-related genes, enhanced the anti-membrane oxidation ability of tobacco, and reduced the oxidative stress damage, thus improving the drought resistance of tobacco.
Identifying hotspots of greenhouse gas emissions from drained peatlands in the European Union
Authors: Giersbergen Quint , Barthelmes Alexandra , Couwenberg john , Fritz Christian , Lång Kristiina , Martin Nina , Tanneberger Franziska 
Published: July 2024

Abstract

Greenhouse gas (GHG) emissions from drained peatlands in the European Union (EU) significantly contribute to the total EU anthropogenic GHG emissions (6%). The lack of high-resolution spatial data in national monitoring systems hampers effective mitigation planning. We present detailed maps of land use, GHG emissions, and emission hotspots for EU peatlands. Results indicate that undrained peatlands and forest lands are prevalent at high latitudes, while grasslands and croplands dominate around latitudes 50°-55°. Three main emission hotspots are identified, all in the North Sea region: South-western England, Western Netherlands, and North-western Germany, accounting for 20% of EU peatland emissions on just 4% of the peatland area. This study highlights the necessity of targeted curbing of emissions from drained peatlands to meet EU climate goals and reveals substantial underreporting of emissions in current National Inventory Submissions to the UNFCCC, amounting to 59-113 Mt CO2-e annually. Our findings provide a crucial basis for policymakers to prioritize peatland rewetting to reduce GHG emissions.
Cellular uptake of Alkyl-Modified Chitosan Nanoparticles by SIM-A9 and hCMEC/D3 cells
Authors: Caprifico Anna E. , Foot Peter , Polycarpou Elena , Calabrese Gianpiero 
Published: July 2024

Abstract

Microglia, the resident macrophages of the brain, become activated during glioblastoma multiforme (GMB) progression and release proinflammatory factors that drive tumor progression. Effective treatment of brain tumors is severely hindered by the blood‒brain barrier (BBB), which is characterized by tightly closed endothelial cells that prevent therapeutic agents from reaching the brain. Nevertheless, chitosan nanoparticles (CsNPs) are known to cross the BBB and confer neuroprotective and anti-inflammatory effects. Therefore, this study aimed to develop CsNPs linked to a lipophilic alkyl chain and assess their cellular uptake for brain drug delivery purposes. Chitosan was chemically modified with butyl glycidyl ether and labeled with fluorescein isothiocyanate (FITC) before generating NPs by ionic gelation. Cellular uptake studies of the NPs were performed on a murine SIM-A9 microglial cell line and an in vitro model of the BBB generated from hCMEC/D3 brain endothelial cells. In particular, microglia were treated with lipopolysaccharide (LPS) to simulate an inflammatory environment typical of brain tumors. Moreover, the intracellular pathways activated by NPs in microglia were investigated by treating cells with sucrose, an inhibitor of clathrin-mediated endocytosis (CME), and by labeling microglia with CD68, which is expressed by lysosomes. Finally, the trypan blue (TB) technique was used to quench the extracellular fluorescence associated with FITC. The results showed that butyl-C NPs were larger (456 ± 5 nm) than CsNPs were (170 ± 10 nm) but were still effectively taken up by the in vitro human BBB model and SIM-A9 cells, even under LPS-activated conditions. NPs did not undergo degradation, while treatment with sucrose did not prevent the uptake of NPs, suggesting that the NPs were engulfed by micropinocytosis. Finally, the TB technique showed that the NPs were effectively localized inside the cytoplasm of the microglia. These data suggested that the butyl chain increased the hydrophobicity, facilitating NP engulfment regardless of size. Due to their lipophilic component, butyl-CsNPs may encapsulate lipophilic drugs to reduce the inflammation associated with activated microglia.
Evaluation of the migration potential of printed plastic films into the aquatic environment
Authors: Edyta  
Published: July 2024

Abstract

This study aimed to evaluate the degree of contamination of water matrices (deionised water, spring water, tap water) in a 14-week experiment using film samples from commercial bags printed with water- and solvent-based inks. The effect of different lighting conditions on leaching and ecotoxicity was also investigated. Samples were exposed for 24 hours to natural sunlight and to no light in a darkroom. A significant increase in contaminants in the aqueous matrices was observed in the 12-week leaching potential of the components from the films in all the lighting variants and aqueous matrices analysed: an increase in dissolved organic carbon (DOC), total organic carbon (TOC) and total suspended solids (TSS). Based on the procedures carried out, the relationship between lighting conditions and the amount of constituents released into the aqueous matrices was not confirmed. The concentration of total organic carbon in all samples peaked between weeks 6 and 9 of the study, followed by a gradual reduction in leaching. This phenomenon could be linked to the gradual depletion of the substances present in the samples or to the achievement of a concentration equilibrium between the aqueous matrix and the sample and/or to the formation of deposits on the surface of the films, which hindered the migration of substances into solutions. Ecotoxicity tests performed between 7 and 14 weeks showed most samples' toxic and highly toxic effects on the growth of Lemna minor fronds and Sinapis alba/Lepidium sativum roots. In contrast, no toxic effects were observed in most samples in screening mortality tests on Daphnia magna and Artemia salina. The study highlights the need for further research into the effects of plastic pollution on the aquatic environment.
ANALISIS POTENSI PENGEMBANGAN PARIWISATA DI KOTA DUMAI
Authors: Fatkhullah Mukhammad , Mulyani Iwed 
Published: July 2024

Abstrak: Penelitian ini mengeksplorasi potensi pengembangan pariwisata di Kota Dumai, Riau, dengan memfokuskan pada sumber daya alam, geografis, sosial, dan budaya yang menjadi daya tarik utama. Melalui jenis penelitian deskriptif dengan pendekatan kualitatif dan metode survei, kami menyelidiki berbagai objek wisata, termasuk pantai, mangrove, gambut, kebun buah, dan warisan budaya melayu. Penelitian juga mengidentifikasi tantangan dan hambatan dalam pengembangan, seperti kebersihan, diversifikasi atraksi, dan aksesibilitas. Hasil penelitian menunjukkan bahwa pengembangan pariwisata di Kota Dumai membutuhkan pendekatan holistik dan kolaboratif antara pemerintah, pengelola wisata, dan masyarakat setempat. Kolaborasi ini diharapkan dapat menciptakan destinasi pariwisata yang berkelanjutan dan memadukan pertumbuhan ekonomi dengan keberlanjutan lingkungan. Potensi kolaborasi dengan perusahaan energi melalui skema CSR juga menjadi peluang strategis dalam menciptakan desa energi yang berkelanjutan. Temuan ini memberikan gambaran umum tentang upaya yang diperlukan untuk mengoptimalkan potensi pariwisata Kota Dumai dan menghadirkan pengalaman wisata yang memuaskan bagi pengunjung lokal maupun internasional.
Kata Kunci: Analisis Kelayakan Wisata; Pengembangan Wisata; Potensi Kota Dumai

Abstract: This research explores the potential for tourism development in Dumai City, Riau, by focusing on the natural, geographical, social, and cultural resources that serve as the main attractions. Through a descriptive research approach with qualitative methods and survey techniques, we investigated various tourist attractions, including beaches, mangroves, peatlands, fruit orchards, and Malay cultural heritage. The study also identified challenges and obstacles in development, such as cleanliness, attraction diversification, and accessibility. The research findings indicate that tourism development in Dumai City requires a holistic and collaborative approach involving the government, tourism managers, and the local community. This collaboration is expected to create a sustainable tourism destination that integrates economic growth with environmental sustainability. The potential collaboration with energy companies through CSR schemes is also seen as a strategic opportunity to establish a sustainable energy village. These findings provide a general overview of the efforts needed to optimize the tourism potential of Dumai City and deliver a satisfying tourist experience for both local and international visitors.
Keywords: Tourism Feasibility Analysis; Tourism Development; Dumai City Potential
Comparing the Soluble Form of Recombinant Human Insulin-like Growth
Factor-1 (rhIGF-1) in Escherichia coli Using Thioredoxin as Fused and
Co-expressed Protein
Authors: Hemmati Sara , Maghami Parvaneh , Ranjbari Javad , Tabarzad Maryam 
Published: July 2024

Introduction:
Insulin-like growth factor-1 (IGF-1) is a single-chain polypeptide with
various physiological functions. Escherichia coli is one of the most desirable hosts for recombinant
protein production, especially for human proteins whose post-translation modifications are
not essential for their bioactivity, such as hIGF-1.

Objectives:
In this study, bacterial thioredoxin (Trx) was studied as a fused and non-fused protein
to convert the insoluble form of recombinant human IGF-1 (rhIGF-1) to its soluble form in E.
coli.

Methods:
The rhIGF-1 was expressed in the E. coli Origami strain in the form of fused-Trx. It
was co-expressed with Trx and then purified and quantified. In the next step, the biological activity
of rhIGF-1 was evaluated by alkaline phosphatase (ALP) activity assay in human adipose-
derived stem cells (hASCs) regarding the differentiation enhancement effect of IGF-1
through the osteogenic process.

Results:
Results showed that Trx in both the fused and non-fused forms had a positive effect on
the production of the soluble form of rhIGF-1. A significant increase in ALP activity in hASCs after
rhIGF-1 treatment was observed, confirming protein bioactivity.

Conclusion:
It was strongly suggested that the overproduction of Trx could increase the solubility
of co-expressed recombinant proteins by changing the redox state in E. coli cells.
A Look at the Etiology of Alzheimer's Disease based on the Brain Ischemia Model
Authors: Pluta Ryszard 
Published: July 2024

:
Alzheimer's disease (AD) is the frequent form of dementia in the world. Despite over
100 years of research into the causes of AD, including amyloid and tau protein, the research has
stalled and has not led to any conclusions. Moreover, numerous projects aimed at finding a cure
for AD have also failed to achieve a breakthrough. Thus, the failure of anti-amyloid and anti-tau
protein therapy to treat AD significantly influenced the way we began to think about the etiology
of the disease. This situation prompted a group of researchers to focus on ischemic brain episodes,
which, like AD, mostly present alterations in the hippocampus. In this context, it has been proposed that cerebral ischemic incidents may play a major role in promoting amyloid and tau protein
in neurodegeneration in AD. In this review, we summarized the experimental and clinical research
conducted over several years on the role of ischemic brain episodes in the development of AD.
Studies have shown changes typical of AD in the course of brain neurodegeneration post-ischemia, i.e., progressive brain and hippocampal atrophy, increased amyloid production, and modification of tau protein. In the post-ischemic brain, the diffuse and senile amyloid plaques and the
development of neurofibrillary tangles characteristic of AD were revealed. The above data evidently showed that after brain ischemia, there are modifications in protein folding, leading to massive
neuronal death and damage to the neuronal network, which triggers dementia with the AD phenotype
Lawsonia inermis from the Algerian Sahara: Quantitative Analysis of
Lawsone in Leaves with Special Attention to their Drying Conditions
Authors: Abdelaziz Berreghioua , Said Bennaceur , Laid Ziane 
Published: July 2024

Introduction:
The continuously growing number of commercial Lawsonia inermis (henna) powders and the wide variability of their quality necessitate verifying as well as controlling their
level of lawsone, the main active ingredient. The purpose of this study is to examine the effect of
drying on the level of lawsone.

Method:
The leaves of henna were dried at different temperatures (40°C, 50°C, and 60°C) with both
modes (air and ulrasons). We extracted lawsone from leaves by a Soxhlet extractor using methanol
and water as solvents, respectively. This natural pigment was isolated, purified, and analyzed by
thin-layer chromatography (TLC), high-pressure liquid chromatography (HPLC), and ultra-violet
(UV) spectrophotometer.

Result:
The drying temperature highly affected the extraction yield (Re). Therefore, drying henna
leaves at low temperatures allows for a higher yield of lawsone extracts.

Conclusion:
We recommend ultrasonically assisted drying of henna leaves to facilitate both the reduction of drying temperatures in order to conserve energy as well as the increase of the yield of
lawsone extraction.
Recent Developments in the Use of Spinel Ferrite Nanoparticles as Catalysts in Organic Reactions
Authors: Kalita Babul , Iraqui Saddam , Dutta Gariyasee , Rashid Md. Harunar 
Published: July 2024

:
The unique physicochemical properties, low cost, low toxicity, and size- and shape-dependent magnetic properties of the ferrite-based
nanoparticles make them an indispensable choice of material for various applications, including catalysis.

:
The objective of this review is to summarize the results of the most widely used ferrite nanoparticles such as NiFe2O4, CuFe2O4, CoFe2O4, ZnFe2O4,
and MnFe2O4 as catalysts in organic reactions such as C–X (X = N, O, and S) coupling, oxidation, and N-heterocycles formation reactions.

:
This review includes a well-summarized compilation of the most widely used nanostructured ferrites such as NiFe2O4, CuFe2O4, CoFe2O4, ZnFe2O4,
and MnFe2O4 as heterogeneous catalysts in selected organic reactions such as C–X (X = N, O, and S) coupling, oxidation, and N-heterocycles
formation reactions. The nanostructured magnetic ferrite catalysts are reliable and extremely effective and facilitate the quick separation of
catalysts, making the process sustainable.

:
The presentation of the review has been proposed anticipating new perspectives and insight in the field of catalysis and to investigate further
development of novel ferrite materials on an industrial scale for practical applications.
The Effect of MiR320a on Lung Cancer
Authors: Hasani Arian 
Published: July 2024

:
Lung cancer has a high mortality rate among cancers in both women and men. Currently, lung cáncer diagnosis is made with clinical examination, low-dose CT scan and molecular-based methods and its treatment options include chemotherapy, surgery, radiotherapy or immunotherapy. However, the life expectancy of lung cancer is not very high, and still it is usually diagnosed very lately, which leads to poorer prognosis. MicroRNAs [miRNAs] are small noncoding RNAs that regulate many diverse activities in the cell that can affect tumorigenesis by regulating many cell functions related to cancer, such as cell cycle, metastasis, angiogenesis, metabolism, and apoptosis. Also, it can have a potential diagnostic, therapeutic, and prognostic value for lung cancer. MiR320a is a promising microRNA that may help us in the diagnosis, treatment and prognosis of lung cancer, but some aspects of its clinical application are still vague, especially its effect on heavy smokers, delivery mechanism, toxicity and lack of reliable critical value. In this paper, we examined its comprehensive molecular interactions that lead to its tumor suppressor effect, and we reviewed its clinical application until now.
Psilocybin in the Management of Substance Use Disorders: A Summary of
Current Evidence
Authors: Bourji Hussein El , Farhat Aziz , Hamdan Zahi , Satodiya Ritvij , Shukla Rashmi , El Hayek Samer 
Published: July 2024

Background:
Following clinical trials on psilocybin for the treatment of pain, anxiety,
and depression in patients with cancer, scientific interest emerged in its use for substance use disorders.

Methods:
In this review of the literature, we summarize available trials looking at the use of psilocybin in addiction

Results:
One double-blind, randomized clinical trial looked at the effect of psilocybin on heavy
drinking in adults diagnosed with alcohol dependence. Several trials are currently ongoing to assess
psilocybin’s efficacy in the management of different SUDs. Otherwise, the current evidence is insufficient to derive any conclusions on the possible efficacy of psilocybin in substance use disorders.

Conclusions:
More well-powered, blinded, randomized controlled trials are needed to investigate
the possible therapeutic effects of psilocybin in addiction while identifying the appropriate conditions that promote its safe use.