bluuebunny/crossref_metadata_2025_split huggingface dataset:
Publicações internacionais sobre sustentabilidade: uma revisão de artigos com o uso da técnica de análise de conteúdo qualitativa
Authors: Froehlich Cristiane 
Published: October 2014

This study aims to identify articles related to sustainability in international publications and analyze the categories that emerge from these studies through the use of the technique of qualitative content analysis in order to identify the main approaches, author contributions and theoretical gaps and suggestions for further studies. For application of the technique have been rising about 20 articles in Journals that feature sustainability impact factor relevant, and used the three basic steps for content analysis of the publications listed by Bardin (1977): (a) pre-analysis; (b) material exploration and; (c) treatment data, inference and interpretation. The main results show that the major theories that are related to sustainability are: the resources and capabilities theory, institutional theory, stakeholders theory, the theory of market orientation, theory of supply chain and competitive advantage and both seek to help and explain the factors that facilitate or hinder the practice of corporate sustainability. In addition, some suggestions for further research were identified and analysis of the results presented in this study.
(Il)légitimité du renseignement financier
Authors: Amicelle1 Anthony 
Published: October 2014

Cet article est consacré à un enjeu encore trop peu étudié malgré son importance croissante dans un domaine tel que le policing transnational, à savoir le renseignement financier. Afin de contribuer à la réflexion sur cette composante à part entière des pratiques contemporaines de surveillance ou plutôt de dataveillance, l’analyse porte plus précisément sur le programme américain de traque du financement du terrorisme (Terrorist Finance Tracking Program [TFTP]). Fondé sur un espace de relations à la fois au-dessus, au-delà et en deçà du national, ce programme de sécurité participe à la production de renseignement à partir d’un dispositif de traçabilité des flux financiers. Il s’agit ici d’expliciter les conditions d’existence d’un tel dispositif consistant à mobiliser de larges ensembles de traces numériques générés par une forme particulière de circulation transfrontière. Il s’agit aussi de mettre la légitimité du TFTP à l’épreuve des narratifs de justification et des « réussites » qui lui sont associés.
Technologies, déqualification et luttes d’influence chez les professionnels de la sécurité frontalière
Authors: Côté-Boucher1 Karine 
Published: October 2014

Les travaux sur la sécurité frontalière ont clairement établi que les technologies se situent au coeur de la reconstitution des activités de police frontalière et de l’espace occupé par les frontières. Cet article a pour but d’ouvrir ces perspectives vers le monde peu étudié des douanes, c’est-à-dire là où l’on se préoccupe de la traçabilité des marchandises et des déplacements des travailleurs du transport. En nous appuyant sur une recherche de terrain menée dans les divisions « commerciales » de cinq postes frontaliers canadiens, nous posons la question des effets concrets de l’intégration des technologies dans les procès de travail douaniers. Nous nous penchons sur la manière dont les douaniers se constituent comme acteurs de sécurité, mais également sur les pratiques quotidiennes des professionnels de la sécurité frontalière, les conditions de travail et les relations intraorganisationnelles au sein de ces agences de sécurité frontalières. En effet, plutôt qu’une efficience accrue qui serait obtenue par le recours aux technologies, cette intégration présente des écueils qui révèlent, en creux, des luttes d’influence entre acteurs de terrain et cadres intermédiaires avec, en trame de fond, la déqualification et l’automatisation du travail douanier.
Biosurfactantes y su papel en la biorremediación de suelos contaminados con plaguicidas
Authors: De la Rosa Cruz Nuvia L. , Sánchez-Salinas Enrique , Ortiz-Hernández Ma. Laura 
Published: October 2014

Resumen
Los biosurfactantes (BS) son un grupo de moléculas de origen microbiano que se caracterizan por ser anfipáticas, es decir que sus moléculas presentan dos partes diferentes, una hidrofóbica y otra hidrofílica. Son un grupo estructuralmente diverso de moléculas tensoactivas, que presentan en general menor toxicidad y mayor biodegradabilidad que los surfactantes sintéticos. Entre las aplicaciones más estudiadas de los BS están aquellas relacionadas con la industria del petróleo y la bioremediación de sitios o residuos contaminados con hidrocarburos. No obstante, los biosurfactantes pueden ser utilizados para otro tipo de compuestos xenobióticos, como es el caso de los plaguicidas. Estos compuestos permiten controlar la proliferación de plagas y enfermedades de los cultivos y del ganado, así como reducir o evitar las pérdidas en la producción de alimentos y contribuir al control de los vectores de diversas enfermedades. No obstante la importancia económica de los plaguicidas, es necesario destacar que su aplicación indiscriminada y sin control ha generado diversos problemas como intoxicación a seres humanos, efectos carcinogénicos, teratogénicos y mutagénicos, además de numerosos problemas ambientales como contaminación de mantos freáticos, aguas continentales y costeras; contaminación del suelo y bioacumulación en las cadenas alimentarias. Generalmente los plaguicidas son de naturaleza hidrofóbica, por lo que el uso de los BS puede ser prometedor en la remediación de sitios contaminados o en el tratamiento biotecnológico de residuos de plaguicidas.
Lubrication Systems
Authors: Jenckes Charles H. 
Published: October 2014

AbstractLubrication is needed to prevent wear or failure of parts in relative motion and in close proximity. A lubricant is placed between the two surfaces to distribute the load and act as a coolant. The lubricant must support the applied load, keep the moving surfaces separated, reduce friction, control the temperature, and carry away debris. Fundamentally, the lubrication system must supply each contact interface within the engine with sufficient lubrication appropriate to each operating condition. The lubricant must be stored, filtered, and supplied to the needed locations to complete its lubrication mission; then cooled, and returned to the filter to close the loop.Lubrication systems range from the simple splash lubrication system to the highly complex dry sump systems used in F1. This chapter covers the basics of automotive lubrication systems.
Application of Radio Wave Technologies to Vehicles
Authors: Naito Hiromichi 
Published: October 2014

Abstract
In the 1930s, the car radio heralded the introduction of vehicle components that use radio waves. Since then, various radio‐wave‐based automotive applications have been developed in accordance with the evolution of semiconductor technology and the spread of automotive electronics. These trends have helped elevate radio waves into a medium capable of data communication within vehicles and linking cars with people and society. This chapter outlines radio wave technologies and describes various automotive components that use radio waves.
Ergonomics for Passenger Cars
Authors: Marek Clemens , Siebertz Karl 
Published: October 2014

AbstractDriving a vehicle is a demanding task, even if it is not done as a profession. Ergonomics therefore plays an important role during the development process. Human limits are manifold, which makes ergonomics a multidisciplinary task. Capacity and demand need to be considered simultaneously. This chapter explains various categories of human performance limits and brings this into an automotive context. International standards are introduced, along with an explanation of the tools and methods to apply the standards. Knowledge about the human body is an essential component of ergonomics. Key findings and methods in the areas of anthropometry, biomechanics, and physiology are described briefly. A practical guide through the layout of the driver's environment shows how the specific knowledge can be applied in order to optimize the ergonomics of passenger vehicles. A few considerations related to vehicle evaluation conclude the chapter. Owing to the complexity of the subject, this chapter can only provide an overview and refers to literature sources for further reading.
Visual Imaging for Diagnosis and Robotic Surgery
Authors: Kumar Rajesh 
Published: October 2014

AbstractVisual imaging has remained the primary modality for manual clinical assessment and intervention in abdominal surgery despite the increasing popularity of radiological imaging‐based diagnosis. This includes endoscopic diagnosis laboratories and laparoscopic and robotic laparoscopic telesurgical procedures. Advances such as wireless capsule endoscopes and robotic surgeries have both provided visual access to organs previously not well imaged and improved the quality and adoption of minimally invasive techniques. This chapter summarizes the technology and applications of visual diagnosis and visually guided robotic surgery along with image analysis methods in development for these images.
Fundamentals of Optics and Radiometry for Color Reproduction
Authors: Hébert Mathieu , Hersch Roger D. , Emmel Patrick 
Published: October 2014

AbstractVisual appearance of objects comes from the interpretation by the human visual system of a light signal issued from the objects. Describing or predicting appearance is, therefore, a question of light and requires notions about light propagation and measurement. In this chapter, we introduce basic laws of optics, the science of light, and radiometry, the science of light measurement, in the context of colored surfaces. We address light spectrum and illuminants, polarization, notions of reflectance and transmittance based on radiometric definitions, gloss, absorption, scattering, fluorescence, and models for light reflection and transmission by flat and rough surfaces and by slabs of nonscattering media and diffusing layers. Throughout the chapter, we present the most current methods to assess the different physical quantities by measurement.
Desorption durch impulsive Anregung intramolekularer Vibrationszustände – eine Methode zur schnellen Extraktion von Proteinen aus intakten Geweben
Authors: Kwiatkowski Marcel , Wurlitzer Marcus , Omidi Maryam , Ren Ling , Kruber Sebastian , Nimer Refat , Robertson Wesley D. , Horst Andrea , Miller R. J. Dwayne , Schlüter Hartmut 
Published: October 2014

AbstractMithilfe eines Pikosekunden‐IR‐Lasers (PIRL) können Proteine in weniger als einer Millisekunde aus intakten Geweben in die Gasphase katapultiert werden. Die ultraschnelle Desorption erfolgt durch eine impulsive Anregung intramolekularer Vibrationszustände (DIVE) der Wassermoleküle, die die Proteine innerhalb des Gewebes umgeben. PIRL‐DIVE extrahiert mit hohen Ausbeuten eine große Vielzahl unterschiedlicher Proteine, von wenigen kDa bis zu mehreren MDa. Die chemische Zusammensetzung der Proteine wird durch die Energie des PIRL nicht verändert. Der Nachweis von Enzymaktivitäten in den PIRL‐Extrakten weist darauf hin, dass die Konformation der Enzyme erhalten bleibt.
Simultaneous In Situ Quantification of Two Cellular Lipid Pools Using Orthogonal Fluorescent Sensors
Authors: Liu Shu‐Lin , Sheng Ren , O'Connor Matthew J. , Cui Yang , Yoon Youngdae , Kurilova Svetlana , Lee Daesung , Cho Wonhwa 
Published: October 2014

AbstractLipids regulate a wide range of biological activities. Since their local concentrations are tightly controlled in a spatiotemporally specific manner, the simultaneous quantification of multiple lipids is essential for elucidation of the complex mechanisms of biological regulation. Here, we report a new method for the simultaneous in situ quantification of two lipid pools in mammalian cells using orthogonal fluorescent sensors. The sensors were prepared by incorporating two environmentally sensitive fluorophores with minimal spectral overlap separately into engineered lipid‐binding proteins. Dual ratiometric analysis of imaging data allowed accurate, spatiotemporally resolved quantification of two different lipids on the same leaflet of the plasma membrane or a single lipid on two opposite leaflets of the plasma membrane of live mammalian cells. This new imaging technology should serve as a powerful tool for systems‐level investigation of lipid‐mediated cell signaling and regulation.
Over 10 000 Peptide Identifications from the HeLa Proteome by Using Single‐Shot Capillary Zone Electrophoresis Combined with Tandem Mass Spectrometry
Authors: Sun Liangliang , Hebert Alexander S. , Yan Xiaojing , Zhao Yimeng , Westphall Michael S. , Rush Matthew J. P. , Zhu Guijie , Champion Matthew M. , Coon Joshua J. , Dovichi Norman J. 
Published: October 2014

AbstractCapillary zone electrophoresis (CZE)–tandem mass spectrometry (MS/MS) has recently attracted attention as a tool for shotgun proteomics. However, its performance for this analysis has so far fallen far below that of reversed‐phase liquid chromatography (RPLC)–MS/MS. The use of a CZE method with a wide separation window (up to 90 min) and high peak capacity (ca. 300) is reported. This method was coupled to an Orbitrap Fusion mass spectrometer through an electrokinetically pumped sheath‐flow interface for the analysis of complex proteome digests. Single‐shot CZE–MS/MS lead to the identification of over 10 000 peptides and 2100 proteins from a HeLa cell proteome digest in approximately 100 min. This performance is nearly an order of magnitude better than earlier CZE studies and is within a factor of two to four of the state‐of‐the‐art nano ultrahigh‐pressure LC system.
Short‐lived Phenoxyl Radicals Formed from Green‐Tea Polyphenols and Highly Reactive Oxygen Species: An Investigation by Time‐Resolved EPR Spectroscopy
Authors: Neshchadin Dmytro , Batchelor Stephen N. , Bilkis Itzhak , Gescheidt Georg 
Published: October 2014

AbstractPolyphenols are effective antioxidants and their behavior has been studied in depth. However, a structural characterization of the species formed immediately upon hydrogen‐atom transfer (HAT), a key reaction of oxidative stress, has not been achieved. The reaction of catechin and green‐tea polyphenols with highly reactive O‐centered H‐abstracting species was studied at the molecular level and in real time by using time‐resolved electron paramagnetic resonance (EPR) spectroscopy. This mirrors the reaction of highly reactive oxygen species with polyphenols. The results show that all phenolic OH groups display essentially identical reactivity. Accordingly, there is no site specificity for HAT and initial antioxidative events are demonstrated to be largely ruled by statistical (entropic) factors.
Study on the interaction between ligustrazine and 12‐tungstophosphoric acid using resonance Rayleigh scattering and resonance nonlinear scattering spectra, and its analytical applications
Authors: Li Junbo , Yang Xiaoli , Yang Jinxiang , Lai Lina 
Published: October 2014

AbstractIn an HCl medium (pH 1.5), ligustrazine (2,3,5,6‐tetramethylpyrazine, TMP) reacted with 12‐tungstophosphoric acid (TP) to form a 3 : 1 ion‐association complex. As a result, the intensities of resonance Rayleigh scattering (RRS), second‐order scattering (SOS) and frequency doubling scattering (FDS) were greatly enhanced and new scattering spectra appeared. The maximum RRS, SOS and FDS wavelengths of the ion‐association complexes were located at 379, 738 and 395 nm, respectively. The scattering intensity increments (ΔIRRS, ΔISOS and ΔIFDS) were directly proportional to the concentration of ligustrazine within certain ranges. The detection limits (3σ) of RRS, SOS and FDS were 1.6, 3.2 and 2.8 ng/mL. Optimal conditions for the RRS method and factors influencing the method were discussed, and the structure of the ion‐association complex and the reaction mechanism were investigated. Transmission electron microscopy (TEM) was used to characterize the structures of the ion‐association complex. Based on the ion‐association reaction and its spectral response, a rapid, simple and sensitive RRS method for the determination of TMP was developed. It was applied to the determination of TMP in tablet and urine samples with satisfactory results. Copyright © 2014 John Wiley & Sons, Ltd.
A combination of targeted toxin technology and the piggyBac‐mediated gene transfer system enables efficient isolation of stable transfectants in nonhuman mammalian cells
Authors: Sato Masahiro , Inada Emi , Saitoh Issei , Matsumoto Yuko , Ohtsuka Masato , Miura Hiromi , Nakamura Shingo , Sakurai Takayuki , Watanabe Satoshi 
Published: October 2014

AbstractIsolation of cells harboring exogenous DNA is typically achieved by the introduction of plasmids, but its efficiency remains still low. In this study, we developed a novel strategy to obtain stable transfectants efficiently. Porcine embryonic fibroblasts were transfected with two plasmids: (i) pTransIEnd, which comprises the ubiquitous promoter, the piggyBac (PB) transposase gene, an internal ribosomal entry site, the Clostridium perfringens‐derived endo‐β‐galactosidase C (EndoGalC) gene, and a poly(A) tail and (ii) a PB‐based plasmid, termed pT‐EGFP, which contains enhanced green fluorescent protein (EGFP) expression unit flanked by PB acceptor sites. The PB transposase can accelerate the chromosomal integration of transposon vectors. EndoGalC expression results in removal of a cell surface α‐Gal epitope, which is specifically recognized by Bandeiraea simplicifolia isolectin‐B4 (IB4). Four days after transfection, cells were treated with IB4SAP (IB4 conjugated to saporin, which eliminates any α‐Gal epitope‐expressing cells) for a short period, followed by standard culture for approximately 10 days. Several colonies emerged, most of which were positive for EGFP expression and lacked TransIEnd. These results indicated that the proposed approach is useful and efficient for obtaining stable transfectants without the use of drug‐resistance genes, and offers a novel route for gene manipulation in cultured nonhuman mammalian cells.
Development and validation of a single HPLC method for determination of α‐tocopherol in cell culture and in human or mouse biological samples
Authors: Cimadevilla Henar M. , Hevia David , Miar Ana , Mayo Juan C. , Lombo Felipe , Sainz Rosa M. 
Published: October 2014

AbstractA straightforward and common analytical method for α‐tocopherol (αT) determination in various biological samples, including plasma, red blood cells (RBC), tissues and cultured cell lines, was developed and validated, using a reverse phase‐chromatographic method (RP‐HPLC). Even though many chromatographic methods for αT determination have been reported, most of them require readjustment when applied to different types of samples. Thus, an effective and simple method for αT determination in different biological matrices is still necessary, specifically for translational research. This method was applied using a C18 column (250 × 4.6 mm, 5 µm particle size) under isocratic elution with MeOH:ACN:H2O (90:9:1 v/v/v) at a flow rate of 1 mL/min and detected using photodiode array at 293 nm. Linearity (r >0.9997) was observed for standard calibration with inter‐ and intraday variation of standard <4%. Lower limits of detection and quantification for αT in this assay were 0.091 and 0.305 µg/mL respectively. Validation proved the method to be selective, linear, accurate and precise. The method was successfully applied in great variety of biological samples, that is, human and mouse plasma, RBCs, murine tissues and human/mouse/rat cultured cell lines. More importantly, a single protocol of extraction and detection can be applied, making this method very convenient for standardization of different types of samples. Copyright © 2014 John Wiley & Sons, Ltd.
Directed Evolution of a Formate Dehydrogenase for Increased Tolerance to Ionic Liquids Reveals a New Site for Increasing the Stability
Authors: Carter Julie L. L. , Bekhouche Mourad , Noiriel Alexandre , Blum Loïc J. , Doumèche Bastien 
Published: October 2014

AbstractThe formate dehydrogenase (FDH) from Candida boidinii is a well‐known enzyme in biocatalysis for NADH regeneration. Nevertheless, it has low activity in a water‐miscible ionic liquid (1,3‐dimethylimidazolium dimethyl phosphate, [MMIm][Me2PO4]). In this work, this enzyme was subjected to directed evolution by using error‐prone PCR, and a mutant (N187S/T321S) displaying higher activity was obtained following selection based on the formazan‐based colorimetric assay. The mutation N187S is responsible for improved activity both in aqueous solution and in [MMIm][Me2PO4], through an enhancement of the kcat value by a factor of 5.8. Fluorescence experiments performed in the presence of a quenching agent revealed that the mutant does not unfold in the presence of 50 % (v/v) [MMIm][Me2PO4] whereas the wild‐type enzyme does. Molecular modelling revealed that the mutation is located at the monomer–monomer interface and causes an increase in the pKa of residue E163 from 4.8 to 5.5. Calculation of the pKa of this residue in other microbial FDHs showed that thermostable FDHs have a highly basic glutamate at this position (pKa up to 6.2). We have identified a new site for improving FDH thermostability and tolerance to ionic liquids, and it is linked to the local charge of the enzymes in this class.
Evidences of Intracrystalline Mesostructured Porosity in Zeolites by Advanced Gas Sorption, Electron Tomography and Rotation Electron Diffraction
Authors: Garcia‐Martinez Javier , Xiao Changhong , Cychosz Katie A. , Li Kunhao , Wan Wei , Zou Xiaodong , Thommes Matthias 
Published: October 2014

AbstractThe front cover artwork for Issue 11/2014 is a collaboration between Rive Technology, Inc. (USA), Stockholm University (Sweden), Quantachrome Instruments (USA), and the University of Alicante (Spain), The image shows that a new family of materials, namely mesostructured zeolites, bridges the gap between conventional (microporous) zeolites and surfactant‐templated (mesoporous) solids. See the Full Paper itself on page http://dx.doi.org/10.1002/cctc.201402499.
Electron Transport through Early Exponential‐Phase Anode‐Grown Geobacter sulfurreducens Biofilms
Authors: Strycharz‐Glaven Sarah M. , Roy Jared , Boyd Darryl , Snider Rachel , Erickson Jeffrey S. , Tender Leonard M. 
Published: October 2014

AbstractGeobacter sulfurreducens biofilms were grown to early exponential phase (i.e. the point at which the catalytic current first begins to increase) on interdigitated microelectrode arrays (IDAs), resulting in the formation of sparse cell clusters surrounded by extracellular polymeric substance (EPS). Continuous domains of EPS (defined here to include extracellular materials including filaments), but not cell clusters, were observed to bridge the gaps between adjacent electrode bands. Electrochemical gate measurements revealed electrical continuity between adjacent IDA electrode bands, indicating that extracellular electron transport (EET) occurs through the EPS. The dependency of the source‐drain current on the gate potential is peak shaped, which is consistent with EPS acting as a redox conductor. The gate potential at which the maximum source‐drain current occurs is approximately 0.13 V more positive than that for stationary‐phase biofilms, indicating that redox cofactors involved in EET at the early exponential phase are different to those at the stationary phase.