bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional response of primary hippocampal neurons following exposure to 3.0 GHz radiofrequency electromagnetic fields
Authors: Cantu Jody C. , Butterworth Joseph W. , Payne Jason A. , Echchgadda Ibtissam 
Published: June 2024

AbstractExposure to radiofrequency (RF) electromagnetic fields (EMF) has been associated with the modulation of neuronal electrophysiology and synaptic plasticity. Given the potential of these changes to coincide with alterations in gene expression, this study investigated whether a transcriptional response would occur in neurons following exposure to RF‐EMF, under both thermal and nonthermal conditions. Rat primary hippocampal neurons (PHNs) underwent either a single (one‐time) or a multiple (3‐times, once a day) exposures to RF‐EMF (3.0 GHz, CW) at two different mean specific absorption rate (SAR) values of 0.57 W/kg or 5.91 W/kg, which induced a temperature change (ΔT °C) of approximately 0.3°C or 3.6°C, respectively. Alteration in transcription in the RF‐EMF‐exposed PHNs versus the sham counterparts was assessed at 0, 4, and 24 h postexposure via high‐throughput RNA sequencing using Illumina HiSeq. 2000. A total of 20 differentially expressed genes (DEGs) exhibited significant upregulation due to RF‐EMF exposure, observed only with the high SAR dose that induced a thermal rise. However, the expression of these DEGs was not significant at 24 h postexposure. Our findings confirmed a lack of nonthermal effects on gene expression under low RF‐EMF exposure conditions as evaluated. Additionally, the results indicated a slight thermal effect of exposures at the dose nearing the standards threshold of 4 W/kg; however, the effect appeared to be transient. The study suggests that RF‐EMF exposures at a level close to the standards threshold, despite inducing mild temperature elevations (i.e., 3–5°C above normal), would not trigger biologically critical cellular changes.
Cross‐cultural adaptation and validation of the Chinese version of Skills and Needs Inventories in Functional Behavior Assessments and Interventions (SNI‐FBAI‐CN)
Authors: Chen Mo , Dutt Anuradha S. , Nair Rahul 
Published: June 2024

AbstractCross‐cultural adaptation and validation of measures are necessary to provide evidence‐based educational intervention services among children with autism spectrum disorder (ASD) across countries. Language plays an integral role in the cross‐cultural adaptation and validation process of measures. Currently, there are limited validated tools in Chinese available to assess special education teachers' skills in functional behavior assessments and interventions in Mainland China to effectively support students with ASD. This study aimed to validate a Chinese version of the Skills and Needs Inventories in Functional Behavior Assessments and Interventions (SNI‐FBAI‐CN) in mainland China. The SNI‐FBAI, originally developed and validated in Singapore, in the English language, was translated, culturally adapted, and then administered to 239 special education teachers in two schools for children with ASD in China. Results show that the SNI‐FBAI‐CN has a three‐factor structure (i.e., skills in behavioral assessment, skills in behavioral interventions, and needs for training) that fits the data well, with good reliability for the overall scale, as well as the three subscales. Partial measurement invariance was established between the Chinese and the original Singapore samples, providing additional construct validity evidence for this tool. Limitations of this study and directions for future research are discussed.
Development of a model for the ethanol concentration limit as a function of temperature and initial substrate concentration using the yeast Saccharomyces cerevisiae
Authors: SILVA Ruan Dionízio , da Silva Flávio Luiz Honorato , de Melo Santos Sharline Florentino , Neto Alberto Brandão Torres , Lopes Julice Dutra , de Assis Cavalcante Josilene 
Published: June 2024

AbstractIn this study, a model was developed to simulate the effect of temperature () and initial substrate concentration () on the ethanol concentration limit () using the yeast Saccharomyces cerevisiae. To achieve this, regressions were performed using data provided by other authors for to establish a model dependent on and capable of predicting results with statistical significance. After constructing the model, a response surface was generated to determine the conditions where reaches higher values: temperatures between 28°C and 32°C and an initial substrate concentration around 200 g/L. Thus, the proposed model is consistent with the observations that increasing temperatures decrease the ethanol concentration obtained, and substrate concentrations above 200 g/L lead to a reduction in ethanol concentration even at low temperatures such as 28°C.
Metabolomic characterization of monoclonal antibody‐producing Chinese hamster lung (CHL)‐YN cells in glucose‐controlled serum‐free fed‐batch operation
Authors: Sukwattananipaat Puriwat , Kuroda Hirotaka , Yamano‐Adachi Noriko , Omasa Takeshi 
Published: June 2024

AbstractThe fast‐growing Chinese hamster lung (CHL)‐YN cell line was recently developed for monoclonal antibody production. In this study, we applied a serum‐free fed‐batch cultivation process to immunoglobulin (Ig)G1‐producing CHL‐YN cells, which were then used to design a dynamic glucose supply system to stabilize the extracellular glucose concentration based on glucose consumption. Glucose consumption of the cultures rapidly oscillated following three phases of glutamine metabolism: consumption, production, and re‐consumption. Use of the dynamic glucose supply prolonged the viability of the CHL‐YN‐IgG1 cell cultures and increased IgG1 production. Liquid chromatography with tandem mass spectrometry‐based target metabolomics analysis of the extracellular metabolites during the first glutamine shift was conducted to search for depleted compounds. The results suggest that the levels of four amino acids, namely arginine, aspartate, methionine, and serine, were sharply decreased in CHL‐YN cells during glutamine production. Supporting evidence from metabolic and gene expression analyses also suggest that CHL‐YN cells acquired ornithine‐ and cystathionine‐production abilities that differed from those in Chinese hamster ovary‐K1 cells, potentially leading to proline and cysteine biosynthesis.
Survival by first‐line therapy and prognostic group among men with metastatic castration‐resistant prostate cancer
Authors: Caram Megan E. V. , Kumbier Kyle , Tsao Phoebe A. , Burns Jennifer , Sparks Jordan B. , Stensland Kristian D. , Reichert Zachery R. , Alumkal Joshi J. , Hollenbeck Brent K. , Shahinian Vahakn , Tsodikov Alexander , Skolarus Ted A. 
Published: June 2024

AbstractIntroductionMetastatic castration‐resistant prostate cancer (mCRPC) is a heterogeneous disease with prognoses varying from months to years at time of castration‐resistant diagnosis. Optimal first‐line therapy for those with different prognoses is unknown.MethodsWe conducted a retrospective cohort study of men in a national healthcare delivery system receiving first‐line therapy for mCRPC (abiraterone, enzalutamide, docetaxel, or ketoconazole) from 2010 to 2017, with follow‐up through 2019. Using commonly drawn prognostic labs at start of mCRPC therapy (hemoglobin, albumin, and alkaline phosphatase), we categorized men into favorable, intermediate, or poor prognostic groups depending on whether they had none, one to two, or all three laboratory values worse than designated laboratory cutoffs. We used Kaplan–Meier methods to examine prostate specific antigen (PSA) progression‐free and overall survival (OS) according to prognostic group and first‐line therapy, and multivariable cox regression to determine variables associated with survival outcomes.ResultsAmong 4135 patients, median PSA progression‐free survival (PFS) was 6.9 months (95% confidence interval [CI] 6.6–7.3), and median OS 18.8 months (95% CI 18.0–19.6), ranging from 5.7 months (95% CI 4.8–7.0) in the poor prognosis group to 31.3 months (95% CI 29.7–32.9) in the favorable group. OS was similar regardless of initial treatment received for favorable and intermediate groups, but worse for those in the poor prognostic group who received ketoconazole (adjusted hazard ratio 2.07, 95% CI 1.2–3.6). PSA PFS was worse for those who received ketoconazole compared to abiraterone across all prognostic groups (favorable HR 1.76, 95% CI 1.34–2.31; intermediate HR 1.78, 95% CI 1.41–2.25; poor HR 8.01, 95% CI 2.93–21.9).ConclusionCommonly drawn labs at mCRPC treatment start may aid in predicting survival and response to therapies, potentially informing discussions with care teams. First‐line treatment selection impacts disease progression for all men with mCRPC regardless of prognostic group, but impacted OS only for men with poor prognosis at treatment start.
Investigating the determinants of fear of cancer recurrence in patients with early‐stage non‐small cell lung cancer: Insights from a restricted cubic spline model
Authors: Liu Man , Liu Lu , Lv Zhuoheng , Mao Yousheng , Liu Yan 
Published: June 2024

AbstractObjectiveThis study aims to investigate the determinants and the dose–response relationship of fear of cancer recurrence among patients with early‐stage non‐small cell lung cancer (NSCLC), aiming to inform prevention and intervention strategies.MethodsEmploying a cross‐sectional design, we analyzed data from 677 postoperative NSCLC patients who received treatment at National Cancer Center between January 2022 and August 2023. Data collection involved a general demographic survey, the Fear of Progression Questionnaire‐Short Form, Hope Herth Index, and Social Support Rating Scale. We employed logistic regression and restricted cubic spline models to identify factors influencing fear of recurrence (FCR).ResultsUnivariate regression analysis identified female gender and having minor children as significant risk factors, while being older than 60, a higher income, and elevated levels of hope and social support emerged as protective factors. Multivariate logistic regression revealed age (OR = 0.392, 95% CI 0.205–0.750), monthly income (OR = 0.016, 95% CI 0.315–0.886), hope level (OR = 0.305, 95% CI 0.187–0.496), and social support (OR = 0.584, 95% CI 0.375–0.908) as independent influencers of recurrence fear. The restricted cubic spline model indicated a nonlinear impact of hope and social support on this fear.ConclusionAnalysis using the restricted cubic spline model underscores the influence of age, income, hope, and social support on FCR, with a nonlinear dose–response relationship evident between hope, social support, and fear. Prioritizing the enhancement of social support before increasing hope levels can rapidly and effectively alleviate FCR.
Overall and non‐lung cancer incidence and mortality in the National Lung Screening Trial: Opportunities for multi‐cancer early detection
Authors: Patel Alpa V. , Chang Ellen T. , Hackshaw Allan , Janes Sam M. , Buist Diana S. M. , Hubbell Earl , Clarke Christina A. , Colditz Graham A. 
Published: June 2024

AbstractBackgroundCurrently recommended cancer screening programs address only part of the overall population cancer burden. Even populations deemed high‐risk for certain individual cancers experience a considerable potential burden of other cancers. However, few published cancer screening trials report the incidence of untargeted cancers.MethodsThe National Lung Screening Trial (NLST), initiated in 2002–2004, was a randomized controlled trial of lung cancer screening in adults with ≥30 pack‐years of smoking. Active follow‐up for incident invasive cancers continued through 2009.ResultsAmong 53,229 NLST subjects (median follow‐up 6.5 years after randomization), the incidence of lung cancer was 615 per 100,000 person‐years (32% of 6142 overall first primary incident invasive cancers), and that of non‐lung cancer was 1327 per 100,000 (68%). Non‐lung cancer incidence exceeded that for lung cancer in all 5‐year age categories and all quintiles of smoking pack‐years. Besides lung cancer, the most common cancers were prostate, breast, colon/rectum, bladder, and head/neck; 23% were smoking‐related cancers, and 54% were cancer types lacking recommended population‐based screening modalities (32% excluding prostate). Non‐lung cancer comprised 48% of 1793 cancer deaths.ConclusionsIn the NLST, only 32% of first primary cancer incidence after study entry was lung, compared with 68% non‐lung. Even in a population at high risk for lung cancer, a single‐cancer screening test misses most cancers. Thus, in combination with existing single‐cancer screening modalities, multi‐cancer screening tests—which address many of the incident non‐lung cancers in this trial—have potential to address a currently inaccessible portion of cancer morbidity and mortality.
Feasibility of atezolizumab and bevacizumab combination regimens in patients with hepatocellular carcinoma and lung cancer taking direct oral anticoagulants
Authors: Nakabori Tasuku , Kunimasa Kei , Kawabata Masaki , Higashi Sena , Mukai Kaori , Kawamura Takahisa , Inoue Takako , Tamiya Motohiro , Nishino Kazumi , Ohkawa Kazuyoshi 
Published: June 2024

AbstractAimAtezolizumab and bevacizumab (Atezo/Bev) combination immunotherapy regimens and direct oral anticoagulants (DOACs) are both associated with bleeding. Therefore, combining Atezo/Bev regimens with DOACs may exacerbate the bleeding risk. This study investigated the feasibility of the Atezo/Bev regimen in patients taking DOACs.MethodsThis retrospective study included 141 patients with unresectable hepatocellular carcinoma (HCC) or advanced lung cancer (LC) treated with Atezo/Bev regimens. Patients who used antithrombotic agents other than DOACs were excluded. Bleeding events during the Atezo/Bev regimen were analyzed.ResultsThe incidence rates of bleeding of any grade in the DOAC (n = 11) and no antithrombotic agent (NAA) (n = 130) groups were 9.1% and 10.8%, respectively, with no significant differences. Moreover, no significant difference was found in the frequency of bleeding of grade ≥3 between the DOAC and NAA groups. No patients in the DOAC group discontinued the Atezo/Bev regimen because of severe bleeding. Although serum albumin levels, with a hazard ratio (HR) of 0.298 (95% confidence interval [CI]: 0.105–0.847), independently contributed to bleeding events (p = 0.023), DOAC administration did not (HR: 1.357; 95% CI: 0.157–10.54; p = 0.770). Among only patients with HCC (n = 59), none of the five patients taking DOACs experienced bleeding events. A high albumin–bilirubin score (HR: 9.083, 95% CI: 1.118–73.76) was associated with bleeding events (p = 0.039).ConclusionsDOACs did not have a considerable effect on bleeding events in the Atezo/Bev regimens for HCC or LC. Under careful surveillance for bleeding, Atezo/Bev regimens may be feasible in patients receiving DOACs.
Development of a prediction model for suicidal ideation in patients with advanced cancer: A multicenter, real‐world, pan‐cancer study in China
Authors: He Yi , Pang Ying , Yang Wenlei , Su Zhongge , Wang Yu , Lu Yongkui , Jiang Yu , Zhou Yuhe , Han Xinkun , Song Lihua , Wang Liping , Li Zimeng , Lv Xiaojun , Wang Yan , Yao Juntao , Liu Xiaohong , Zhou Xiaoyi , He Shuangzhi , Zhang Yening , Song Lili , Li Jinjiang , Wang Bingmei , Ke Yang , He Zhonghu , Tang Lili 
Published: June 2024

AbstractBackgroundPatients diagnosed with advanced stage cancer face an elevated risk of suicide. We aimed to develop a suicidal ideation (SI) risk prediction model in patients with advanced cancer for early warning of their SI and facilitate suicide prevention in this population.Patients and MethodsWe consecutively enrolled patients with multiple types of advanced cancers from 10 cancer institutes in China from August 2019 to December 2020. Demographic characteristics, clinicopathological data, and clinical treatment history were extracted from medical records. Symptom burden, psychological status, and SI were assessed using the MD Anderson Symptom Inventory (MDASI), Hospital Anxiety and Depression Scale (HADS), and Patient Health Questionnaire‐9 (PHQ‐9), respectively. A multivariable logistic regression model was employed to establish the model structure.ResultsIn total, 2814 participants were included in the final analysis. Nine predictors including age, sex, number of household members, history of previous chemotherapy, history of previous surgery, MDASI score, HADS‐A score, HADS‐D score, and life satisfaction were retained in the final SI prediction model. The model achieved an area under the curve (AUC) of 0.85 (95% confidential interval: 0.82–0.87), with AUCs ranging from 0.75 to 0.95 across 10 hospitals and higher than 0.83 for all cancer types.ConclusionThis study built an easy‐to‐use, good‐performance predictive model for SI. Implementation of this model could facilitate the incorporation of psychosocial support for suicide prevention into the standard care of patients with advanced cancer.
Sydenham's chorea in a 16‐year‐old female from Bhutan: A case report
Authors: Penjor Tshering , Dorji Thinley , Wangchuk Sangay 
Published: June 2024

Key Clinical MessageRheumatic heart disease is a preventable disease. Patients may not present with a typical history of sore throat and polyarthritis but may present with Sydenham's chorea. We should not rely completely on clinical findings to rule out carditis. Echocardiography should be done to rule out subclinical carditis.AbstractSydenham's chorea is a major manifestation of rheumatic fever. It occurs primarily in children and is seen rarely after the age of 20 years. We describe a 16‐year‐old girl who presented with purposeless involuntary movements of her upper and lower limbs. Laboratory blood reports showed raised erythrocyte sedimentation rate and anti‐streptolysin O. 2D Doppler Echocardiography confirmed subclinical carditis, thickened mitral and aortic valve with mild mitral regurgitation. She was managed as Acute Rheumatic Fever with oral Phenoxymethyl penicillin and Carbamazepine. At the latest follow‐up interviewing the caregiver, the patient had no sequelae. Early diagnosis is key to preventing late consequences of acute rheumatic fever and rheumatic heart disease. Sydenham's chorea is a rare presentation of acute rheumatic fever. The absence of clinical carditis does not rule out carditis.
Total talar replacement for metastatic pulmonary adenocarcinoma of the talus: A case report
Authors: Sakai Tatsuya , Fujii Masanori , Mawatari Masaaki 
Published: June 2024

Key Clinical MessageTalar metastases from malignant tumors are rare and poorly documented. Treatment requires gradual relief of pain and preservation of function, with a choice between palliative measures and surgery. This case indicates that total talar replacement is an effective intervention for localized talar metastases and highlights the importance of early intervention. A 48‐year‐old man was diagnosed with a pathologic talar fracture due to talar metastases was observed after 8 years of chemotherapy following a diagnosis of lung adenocarcinoma. Despite radiotherapy, the patient's activities of daily living (ADLs) deteriorated due to pain on walking, prompting a request for surgical intervention. Total talar replacement was performed, allowing the patient to begin full weight‐bearing ambulation 2 weeks post‐operatively. Total talar replacement appears to be an effective treatment for localized talar metastases and should be performed as early as possible.
Hypoxia drives CBR4 down‐regulation promotes gastroenteropancreatic neuroendocrine tumors via activation mammalian target of rapamycin mediated by fatty acid synthase
Authors: Ye Mujie , Xu Lin , Lu Feiyu , Chen Lingyi , Hu Chunhua , Chen Jinhao , Xue Bingyan , Gu Danyang , Xu Ruitong , Xu Yanling , Yu Ping , Wang Yan , Tian Ye , Zhu Guoqin , Tang Qiyun 
Published: June 2024

AbstractHypoxia has been highly proven a hallmark of tumor micro‐environment, promoting the malignant phenotypes, playing a crucial role from tumor initiation, progression, invasion, and intravasation to metastatic dissemination and outgrowth. Increasing evidence also showed that hypoxia mediated the abnormal lipid metabolism in cancer by regulating various oncogenic signal pathways. However, it is still unclear but attractive how hypoxia specifically functioned and changed the condition of the tumor micro‐environment. In present study, we find that hypoxia promoted the methylation degree of CBR4 promoter region thus downgraded the expression of CBR4, which promoted GEP‐NETs progression and increased the sensitivity of GEP‐NETs cells to everolimus. Further, CBR4 interacted with fatty acid synthase (FASN), displaying a down‐regulation of FASN by activating the ubiquitin proteasome pathway and suppressed mTOR signaling. Overall, our results uncovers the CBR4/FASN/mTOR axis as a mechanism for tumor development and inspires us a new molecular guide for the therapeutic strategies for GEP‐NETs treatment.
Symmetry‐Breaking and Symmetry‐Retaining Morphological Evolution of the Single Crystals of Cyclodextrin Metal‐Organic Frameworks
Authors: Zhang Jiayin , Chen Yifu , Zhang Ying‐Ming , Gong Junbo 
Published: June 2024

AbstractThe morphological symmetry‐retaining and symmetry‐breaking of single crystals of the γ‐cyclodextrin metal‐organic framework have been achieved via introducing lower symmetric β‐cyclodextrins and α‐cyclodextrins, respectively. β‐cyclodextrins led to a morphological evolution with retained symmetry from cubic to rhombic dodecahedra, while α‐cyclodextrins resulted in the original cubic crystal missing a vertex angle presenting symmetry‐breaking behavior. The crystal structures of rhombic dodecahedra and angle‐deficient crystals were confirmed through X‐ray crystallography, and the mechanisms underlying the morphological transformation evolution were further analyzed. Our work not only provides a rare case realizing two different paths of morphological evolution in one system, but also encourages future efforts towards the evolution of artificial crystal systems in a natural way.
Clathrate Hydrates as a Kind of Promising Ice Nanoreactors
Authors: Chang Ruisi , Cheng Shibo , Bu Yuxiang 
Published: June 2024

AbstractSince their discovery, clathrate hydrates (CHs) have received great attention both from theoretical and experimental aspects due to their great potential for gas storage and prospective applications as icy crystal materials. However, there has been limited research on the decomposition, reduction or other reactions of gases enclosed in CHs. Thanks to their unique hydrogen bonding network and cavity structures, CHs can serve as the promising nanoreactors to achieve chemical conversions, e. g. reducing greenhouse gases. In this review‐type article, we characterize the potential performance of such CHs nanoreactors by discussing their multiple functions including important roles of hydrogen bonds in CHs, e. g. the confinement effect and proton source, and then discuss the enhanced electron‐binding ability of guest molecules and the structures and properties of trapped electrons in the stacked nanocages, which contribute to our understanding of chemical reactions occurring in CHs. Finally, we provide detailed analyses of representative reaction mechanisms underwent in CH nanoreactors and effective calculational and molecular dynamics simulation methods. This review‐type article aims to provide a detailed summary about the functional characteristics of CHs and reactivity in CHs, which make CHs a kind of promising icy nanoreactors.