bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crown ether helical peptides are preferentially inserted in lipid bilayers as a transmembrane ion channels
Authors: Savoie Jean‐Daniel , Otis François , Bürck Jochen , Ulrich Anne S. , Voyer Normand 
Published: March 2015

ABSTRACTOriented circular dichroism was used to study the alignment crown ether‐modified peptides. The influence of different N‐ and C‐functionalities was assessed using at variable peptide:lipid ratios from 1:20 to 1:200. Neither the functionalities nor the concentration had any major effect on the orientation. The alignment of the 21‐mer peptides was also examined with lipid membranes of different bilayer thickness. The use of synchrotron radiation as light source allowed the study of peptide:lipid molar ratios from 1:20 to 1:1000. For all conditions studied, the peptides were found to be predominantly incorporated as a transmembrane helix into the membrane, especially at low peptide concentration, but started to aggregate on the membrane surface at higher peptide:lipid ratios. The structural information on the preferred trans‐bilayer alignment of the crown ether functional groups explains their ion conductivity and is useful for the further development of membrane‐active nanochemotherapeutics. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 427–433, 2015.
Azide‐rich peptides via an on‐resin diazotransfer reaction
Authors: Marine Jeannette E. , Liang Xiaoli , Song Shuang , Rudick Jonathan G. 
Published: March 2015

ABSTRACTAzide‐containing amino acids are valuable building blocks in peptide chemistry, because azides are robust partners in several bioorthogonal reactions. Replacing polar amino acids with apolar, azide‐containing amino acids in solid‐phase peptide synthesis can be tricky, especially when multiple azide residues are to be introduced in the amino acid sequence. We present a strategy for effectively incorporating multiple azide‐containing residues site‐specifically. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 419–426, 2015.
Solid‐phase synthesis, characterization, and cellular activities of collagen‐model Nanodiamond‐peptide conjugates
Authors: Knapinska Anna M. , Tokmina‐Roszyk Dorota , Amar Sabrina , Tokmina‐Roszyk Michal , Mochalin Vadym N. , Gogotsi Yury , Cosme Patrick , Terentis Andrew C. , Fields Gregg B. 
Published: March 2015

AbstractNanodiamonds (NDs) have received considerable attention as potential drug delivery vehicles. NDs are small (∼5 nm diameter), can be surface modified in a controllable fashion with a variety of functional groups, and have little observed toxicity in vitro and in vivo. However, most biomedical applications of NDs utilize surface adsorption of biomolecules, as opposed to covalent attachment. Covalent modification provides reliable and reproducible ND–biomolecule ratios, and alleviates concerns over biomolecule desorption prior to delivery. The present study has outlined methods for the efficient solid‐phase conjugation of ND to peptides and characterization of ND–peptide conjugates. Utilizing collagen‐derived peptides, the ND was found to support or even enhance the cell adhesion and viability activities of the conjugated sequence. Thus, NDs can be incorporated into peptides and proteins in a selective manner, where the presence of the ND could potentially enhance the in vivo activities of the biomolecule it is attached to. © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 186–195, 2015.
Long‐term follow‐up is not indicated after routine interventional closure of persistent arterial ducts
Authors: Narayan Srinivas A. , Elmahdi Elfadil , Rosenthal Eric , Qureshi Shakeel A. , Krasemann Thomas 
Published: March 2015

BackgroundLittle is known about the necessity for long‐term follow‐up after interventional closure of persistent arterial duct (PDA). Potential side effects and complications include residual shunts, haemolysis, device embolization, and obstruction to flow in the adjoining vessels.MethodsSingle centre retrospective study of paediatric patients undergoing interventional PDA occlusion.Results315 patients who underwent interventional occlusion of a PDA between November 2002 and September 2013 were included. Of these, eight needed re‐intervention (three for device embolization, five for residual shunt). Seven had mild obstruction to flow in the adjoining vessels, but did not require any intervention. All sequelae were found latest at the first follow‐up appointment after the procedure (usually within 3 months); whilst none developed during further follow‐up.ConclusionComplications of interventional closure of PDA were apparent immediately after the procedure or by three months of follow‐up. Long‐term follow‐up is not indicated in cases when no complications are seen early after the procedure. © 2015 Wiley Periodicals, Inc.
A prospective, postmarket study with the Mguard Prime Embolic Protection Stent in ST‐segment elevation myocardial infarction: The International MGuard Prime Observational Study (IMOS Prime)
Authors: Amoroso Giovanni , Vos Nicola S. , Van der Heyden Jan A.S. , van der Schaaf Renė J. , Patterson Mark S. , Vink Maarten A. , Herrman Jean‐Paul , Slagboom Ton 
Published: March 2015

AimsWe sought to evaluate the procedural and clinical performances of the MGuard Prime Embolic Protection Stent (EPS) in a “real‐world” population with acute ST‐segment elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PPCI).Methods and ResultsConsecutive STEMI patients meeting the inclusion criteria and undergoing PPCI were enrolled. The primary endpoint was major adverse cardiac events (MACE) at 30 days, defined as the composite of cardiac death, myocardial infarction (Q wave and non‐Q wave) or target lesion revascularization (PCI or coronary artery bypass graft). Secondary endpoints included device success, lesion success, and postprocedural reperfusion outcomes: thrombolysis in myocardial infarction (TIMI) flow and ST‐segment elevation resolution (STR). A total of 97 patients (62 years, 77% men) were included. Symptom to cathlab time was 238 min. Device and lesion success were 100%. Final TIMI‐3 flow was achieved in 91.8%, and STR > 50% in 87% of the patients. MACE at 30 days was 2.2%, which consisted of one case each of target vessel (TV)‐myocardial infarction (MI), and non–TV‐MI.ConclusionsThe use of the MGuard Prime EPS stent is feasible and safe and could be also effective in achieving myocardial reperfusion in STEMI patients undergoing PPCI. © 2015 Wiley Periodicals, Inc.
In‐hospital mortality risk prediction after percutaneous coronary interventions: Validating and updating the toronto score in Brazil
Authors: Lodi‐Junqueira Lucas , da Silva José L.P. , Ferreira Lorena R. , Gonçalves Humberto L. , Athayde Guilherme R.S. , Gomes Thalles O. , Borges Júlio C. , Nascimento Bruno R. , Lemos Pedro A. , Ribeiro Antônio L.P. 
Published: March 2015

ObjectivesWe aimed to assess the accuracy of the simple, contemporary and well‐designed Toronto PCI mortality risk score in ICP‐BR registry, the first Brazilian PCI multicenter registry with follow‐up information.BackgroundEstimating percutaneous coronary intervention (PCI) mortality risk by a clinical prediction model is imperative to help physicians, patients and family members make informed clinical decisions and optimize participation in the consent process, reducing anxiety and improving quality of care. At a healthcare system level, risk prediction scores are essential to measure and benchmark performance.MethodsBetween 2009 and 2013, a cohort of 4,806 patients from the ICP‐BR registry, treated with PCI in eight tertiary referral medical centers, was included in the analysis. This population was compared to 10,694 patients of the derivation dataset from the Toronto study. To assess predictive performance, an update of the model was performed by three different methods, which were compared by discrimination, calculating the area under the receiver operating characteristic curve (AUC), and by calibration, assessed through Hosmer–Lemeshow (H‐L) test and graphical analysis.ResultsDeath occurred in 2.6% of patients in the ICP‐BR registry and in 1.3% in the Toronto cohort. The median age was 64 and 63 years, 23.8 and 32.8% were female, 28.6 and 32.3% were diabetics, respectively. Through recalibration of intercept and slope (AUC = 0.8790; H‐L P value = 0.3132), we achieved a well‐calibrated and well‐discriminative model.ConclusionsAfter updating to our dataset, we demonstrated that the Toronto PCI in‐hospital mortality risk score performed well in Brazilian hospitals. © 2015 Wiley Periodicals, Inc.
Organization of TNIK in dendritic spines
Authors: Burette Alain C. , Phend Kristen D. , Burette Susan , Lin Qingcong , Liang Musen , Foltz Gretchen , Taylor Noël , Wang Qi , Brandon Nicholas J. , Bates Brian , Ehlers Michael D. , Weinberg Richard J. 
Published: March 2015

ABSTRACTTumor necrosis factor receptor‐associated factor 2 (TRAF2)‐ and noncatalytic region of tyrosine kinase (NCK)‐interacting kinase (TNIK) has been identified as an interactor in the psychiatric risk factor, Disrupted in Schizophrenia 1 (DISC1). As a step toward deciphering its function in the brain, we performed high‐resolution light and electron microscopic immunocytochemistry. We demonstrate here that TNIK is expressed in neurons throughout the adult mouse brain. In striatum and cerebral cortex, TNIK concentrates in dendritic spines, especially in the vicinity of the lateral edge of the synapse. Thus, TNIK is highly enriched at a microdomain critical for glutamatergic signaling. J. Comp. Neurol. 523:1913–1924, 2015 © 2015 Wiley Periodicals, Inc.
Color reproduction on shrink sleeves
Authors: Babaei Vahid , Pelvet Cédric , Hersch Roger D. 
Published: March 2015

AbstractWrapping heat‐deformable plastic labels around packages relies on a shrinking process. Shrinking plastic labels distorts not only the shape but also the color of the printed artwork. In this study, we analyze and model the color shifts induced by shrinkage. The ultimate goal is to generate full color images which after shrinking have colors as close as possible to the original colors. For this purpose, we present a thickness enhanced Clapper–Yule prediction model. Its calibration requires spectral measurements of original, nonshrunk samples, as well as the measured shrinkage factors. With the prediction model, we establish a table creating the correspondences between target colors after shrinking and ink area coverages. This enables creating color images which after shrinking match the original images. © 2015 Wiley Periodicals, Inc. Col Res Appl, 41, 56–63, 2016
The Epidemiology of Nonsurgical Hypoparathyroidism in Denmark: A Nationwide Case Finding Study
Authors: Underbjerg Line , Sikjaer Tanja , Mosekilde Leif , Rejnmark Lars 
Published: March 2015

ABSTRACT

Nonsurgical hypoparathyroidism (HypoPT) is a rare disorder most often caused by mutations in different genes. It is characterized by hypocalcaemia with inappropriately low PTH levels. Knowledge about this group of patients, including their mortality and morbidity, is very sparse. The aim was to identify all patients diagnosed with nonsurgical HypoPT in Denmark and assess their mortality and risk of complications. Through registers and review of individual patient hospital charts we identified all patients diagnosed with nonsurgical HypoPT in Denmark between 1977 and 2012. We assessed their mortality and morbidity by comparing them with a group of age- and gender-matched population-based controls. We identified a total of 180 patients with nonsurgical HypoPT among whom 123 (68%) were alive at the date of follow-up (prevalence of 2.3/100,000 inhabitants). Compared with controls, mortality was not increased, but patients had a significantly increased risk of renal insufficiency (hazard ratio [HR] 6.01), cardiovascular diseases (HR 1.91), neuropsychiatric complications (HR 2.45), infections (HR 1.94), seizures (HR 10.05), cataract (HR 4.21), and fractures at the upper extremities (HR 1.93). In contrast patients had significantly reduced risk of malignant diseases (HR 0.44). In conclusion, nonsurgical HypoPT is a rare disease associated with a number of complications that should be considered when taking care of these patients. © 2015 American Society for Bone and Mineral Research.
Fluocinolone Acetonide Is a Potent Synergistic Factor of TGF-β3–Associated Chondrogenesis of Bone Marrow–Derived Mesenchymal Stem Cells for Articular Surface Regeneration
Authors: Hara Emilio Satoshi , Ono Mitsuaki , Pham Hai Thanh , Sonoyama Wataru , Kubota Satoshi , Takigawa Masaharu , Matsumoto Takuya , Young Marian F , Olsen Bjorn R , Kuboki Takuo 
Published: March 2015

ABSTRACT

Articular cartilage repair remains a challenging problem. Based on a high-throughput screening and functional analysis, we found that fluocinolone acetonide (FA) in combination with transforming growth factor beta 3 (TGF-β3) strongly potentiated chondrogenic differentiation of human bone marrow–derived mesenchymal stem cells (hBMSCs). In an in vivo cartilage defect model in knee joints of immunocompromised mice, transplantation of FA/TGF-β3–treated hBMSCs could completely repair the articular surface. Analysis of the intracellular pathways revealed that FA enhanced TGF-β3–induced phosphorylation of Smad2 and Smad3. Additionally, we performed a pathway array and found that FA activates the mTORC1/AKT pathway. Chemical inhibition of mTORC1 with rapamycin substantially suppressed FA effect, and inhibition of AKT completely repressed chondrogenesis of hBMSCs. Inhibition of glucocorticoid receptor with mifepristone also suppressed FA effect, suggesting that FA involves binding to the glucocorticoid receptor. Comparative analysis with other glucocorticoids (triamcinolone acetonide [TA] and dexamethasone [DEX]) revealed the unique ability of FA to repair articular cartilage surgical defects. Analysis of intracellular pathways showed that the mTORC1/AKT pathway and the glucocorticoid receptor was highly activated with FA and TA, but to a lesser extent with DEX. Collectively, these results show a unique ability of FA to enhance TGF-β3–associated chondrogenesis, and suggest that the FA/TGF-β3 combination may be used as major inducer of chondrogenesis in vitro. Additionally, FA/TGF-β3 could be potentially applied in a clinical setting to increase the efficiency of regenerative approaches based on chondrogenic differentiation of stem cells. © 2015 American Society for Bone and Mineral Research.
The effect of dissolved oxygen on the susceptibility of blood
Authors: Berman Avery J.L. , Ma Yuhan , Hoge Richard D. , Pike G. Bruce 
Published: March 2015

PurposeIt has been predicted that, during hyperoxia, excess O2 dissolved in arterial blood will significantly alter the blood's magnetic susceptibility. This would confound the interpretation of the hyperoxia‐induced blood oxygenation level‐dependent signal as arising solely from changes in deoxyhemoglobin. This study, therefore, aimed to determine how dissolved O2 affects the susceptibility of blood.Theory and MethodsWe present a comprehensive model for the effect of dissolved O2 on the susceptibility of blood and compare it with another recently published model, referred to here as the ideal gas model (IGM). For validation, distilled water and samples of bovine plasma were oxygenated over a range of hyperoxic O2 concentrations and their susceptibilities were determined using multiecho gradient echo phase imaging.ResultsIn distilled water and plasma, the measured changes in susceptibility were very linear, with identical slopes of 0.062 ppb/mm Hg of O2. This change was dramatically less than previously predicted using the IGM and was close to that predicted by our model. The primary source of error in the IGM is the overestimation of the volume fraction occupied by dissolved O2.ConclusionUnder most physiological conditions, the susceptibility of dissolved O2 can be disregarded in MRI studies employing hyperoxia. Magn Reson Med 75:363–371, 2016. © 2015 Wiley Periodicals, Inc.
Free‐breathing, motion‐corrected, highly efficient whole heart T2 mapping at 3T with hybrid radial‐cartesian trajectory
Authors: Yang Hsin‐Jung , Sharif Behzad , Pang Jianing , Kali Avinash , Bi Xiaoming , Cokic Ivan , Li Debiao , Dharmakumar Rohan 
Published: March 2015

PurposeTo develop and test a time‐efficient, free‐breathing, whole heart T2 mapping technique at 3.0T.MethodsECG‐triggered three‐dimensional (3D) images were acquired with different T2 preparations at 3.0T during free breathing. Respiratory motion was corrected with a navigator‐guided motion correction framework at near perfect efficiency. Image intensities were fit to a monoexponential function to derive myocardial T2 maps. The proposed 3D, free breathing, motion‐corrected (3D‐FB‐MoCo) approach was studied in ex vivo canine hearts and kidneys, healthy volunteers, and canine subjects with acute myocardial infarction (AMI).ResultsEx vivo T2 values from proposed 3D T2‐prep gradient echo were not different from two‐dimensional (2D) spin echo (P = 0.7) and T2‐prep balanced steady‐state free precession (bSSFP) (P = 0.7). In healthy volunteers, compared with 3D‐FB‐MoCo and breath‐held 2D T2‐prep bSSFP (2D‐BH), non–motion‐corrected (3D‐FB‐Non‐MoCo) myocardial T2 was longer, had a larger coefficient of variation (COV), and had a lower image quality (IQ) score (T2 = 40.3 ms, COV = 38%, and IQ = 2.3; all P < 0.05). Conversely, the mean and COV and IQ of 3D‐FB‐MoCo (T2 = 37.7 ms, COV = 17%, and IQ = 3.5) and 2D‐BH (T2 = 38.0 ms, COV = 15%, and IQ = 3.8) were not different (P = 0.99, P = 0.74, and P = 0.14, respectively). In AMI, T2 values and edema volumes from 3D‐FB‐MoCo and 2D‐BH were closely correlated (R2 = 0.88 and 0.96, respectively).ConclusionThe proposed whole heart T2 mapping approach can be performed within 5 min with similar accuracy to that of the 2D‐BH T2 mapping approach. Magn Reson Med 75:126–136, 2016. © 2015 Wiley Periodicals, Inc.
Disambiguating the optic nerve from the surrounding cerebrospinal fluid: Application to MS‐related atrophy
Authors: Harrigan Robert L. , Plassard Andrew J. , Bryan Frederick W. , Caires Gabriela , Mawn Louise A. , Dethrage Lindsey M. , Pawate Siddharama , Galloway Robert L. , Smith Seth A. , Landman Bennett A. 
Published: March 2015

PurposeOur goal is to develop an accurate, automated tool to characterize the optic nerve (ON) and cerebrospinal fluid (CSF) to better understand ON changes in disease.MethodsMulti‐atlas segmentation is used to localize the ON and sheath on T2‐weighted MRI (0.6 mm3 resolution). A sum of Gaussian distributions is fit to coronal slice‐wise intensities to extract six descriptive parameters, and a regression forest is used to map the model space to radii. The model is validated for consistency using tenfold cross‐validation and for accuracy using a high resolution (0.4 mm2 reconstructed to 0.15 mm2) in vivo sequence. We evaluated this model on 6 controls and 6 patients with multiple sclerosis (MS) and a history of optic neuritis.ResultsIn simulation, the model was found to have an explanatory R‐squared for both ON and sheath radii greater than 0.95. The accuracy of the method was within the measurement error on the highest possible in vivo resolution. Comparing healthy controls and patients with MS, significant structural differences were found near the ON head and the chiasm, and structural trends agreed with the literature.ConclusionThis is a first demonstration that the ON can be exclusively, quantitatively measured and separated from the surrounding CSF using MRI. Magn Reson Med 75:414–422, 2016. © 2015 Wiley Periodicals, Inc.
Pulse sequence programming in a dynamic visual environment: SequenceTree
Authors: Magland Jeremy F. , Li Cheng , Langham Michael C. , Wehrli Felix W. 
Published: March 2015

PurposeTo describe SequenceTree, an open source, integrated software environment for implementing MRI pulse sequences and, ideally, exporting them to actual MRI scanners. The software is a user‐friendly alternative to vendor‐supplied pulse sequence design and editing tools and is suited for programmers and nonprogrammers alike.MethodsThe integrated user interface was programmed using the Qt4/C++ toolkit. As parameters and code are modified, the pulse sequence diagram is automatically updated within the user interface. Several aspects of pulse programming are handled automatically, allowing users to focus on higher‐level aspects of sequence design. Sequences can be simulated using a built‐in Bloch equation solver and then exported for use on a Siemens MRI scanner. Ideally, other types of scanners will be supported in the future.ResultsSequenceTree has been used for 8 years in our laboratory and elsewhere and has contributed to more than 50 peer‐reviewed publications in areas such as cardiovascular imaging, solid state and nonproton NMR, MR elastography, and high‐resolution structural imaging.ConclusionSequenceTree is an innovative, open source, visual pulse sequence environment for MRI combining simplicity with flexibility and is ideal both for advanced users and users with limited programming experience. Magn Reson Med 75:257–265, 2016. © 2015 Wiley Periodicals, Inc.
Assessment of lymphatic impairment and interstitial protein accumulation in patients with breast cancer treatment‐related lymphedema using CEST MRI
Authors: Donahue Manus J. , Donahue Paula C.M. , Rane Swati , Thompson Christopher R. , Strother Megan K. , Scott Allison O. , Smith Seth A. 
Published: March 2015

PurposeLymphatic impairment is known to reduce quality of life in some of the most crippling diseases of the 21st century, including obesity, lymphedema, and cancer. However, the lymphatics are not nearly as well‐understood as other bodily systems, largely owing to a lack of sensitive imaging technologies that can be applied using standard clinical equipment. Here, proton exchange‐weighted MRI is translated to the lymphatics in patients with breast cancer treatment‐related lymphedema (BCRL).MethodsHealthy volunteers (N = 8) and BCRL patients (N = 7) were scanned at 3 Tesla using customized structural MRI and amide proton transfer (APT) chemical exchange saturation transfer (CEST) MRI in sequence with the hypothesis that APT effects would be elevated in lymphedematous tissue. APT contrast, lymphedema stage, symptomatology, and histology information were evaluated.ResultsNo significant difference between proton‐weighted APT contrast in the right and left arms of healthy controls was observed. An increase in APT contrast in the affected arms of patients was found (P = 0.025; Cohen's d = 2.4), and variability among patients was consistent with documented damage to lymphatics as quantified by lymphedema stage.ConclusionAPT CEST MRI may have relevance for evaluating lymphatic impairment in patients with BCRL, and may extend to other pathologies where lymphatic compromise is evident. Magn Reson Med 75:345–355, 2016. © 2015 Wiley Periodicals, Inc.