bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in breeding phenology of eastern Ontario frogs over four decades
Authors: Klaus Samantha P. , Lougheed Stephen C. 
Published: February 2013

AbstractGlobal climate change has been implicated in phenological shifts for a variety of taxa. Amphibian species in particular are sensitive to changes in their environment due to their biphasic life history and restricted reproductive requirements. Previous research has shown that not all temperate amphibian species respond similarly to the same suite of climatic or environmental cues, nor are individual species necessarily uniform in their responses across their range. We examined both the timing of spring emergence and calling phenology of eight anuran species in southeastern Ontario, Canada, using an approximately 40‐year dataset of historical records of amphibian activity. Rana pipiens was the only species out of eight considered to emerge significantly earlier, by an estimated 22 days over four decades. Both R. pipiens and Bufo americanus have advanced initiation of calling over a four‐decade span significantly earlier by an estimated 37.2 and 19.2 days, respectively. Rana sylvatica showed a trend toward earlier emergence by 19 days, whereas we did not detect changes in emergence phenology for the remaining five species. This significant shift in breeding behavior for two species correlates to significant regional increases in spring temperatures of an estimated 2.7–2.8°C overall over four decades. Our study suggests that local temperature increases have affected the timing of emergence and the onset of calling activity in some Ontario anuran species. Global decline or range shifts ultimately may be related to changes in reproductive behavior and timing mediated by shifting climate.
Triple Click to Tripodal Triazole‐Based Ligands – Synthesis and Characterization of Blue‐Emitting Ce3+ Complexes
Authors: Baschieri Andrea , Mazzanti Andrea , Stagni Stefano , Sambri Letizia 
Published: February 2013

AbstractTris(aryltriazolylmethyl)amine compounds have been synthesized by a one‐pot triple click reaction of azides from anilines with tripropargylamine. The resulting tripodal ligands have been employed to obtain six new Ce3+ complexes. The formation of the new complexes, which were obtained both as heteroleptic or homoleptic types, is influenced by the nature of the counterion (nitrate, perchlorate, or triflate) of the starting Ce3+ salts. All the new Ce3+‐based complexes are highly stable both in solution and in the solid state. Their structural features have been at first studied by NMR spectroscopy. In addition, by combining experimental and DFT theoretical approaches, the X‐ray structure of one heteroleptic complex has been used to simulate the structures of all the remaining complexes. Finally, both the heteroleptic and the homoleptic complexes are luminescent, and the analysis of their photophysical properties indicate that the Ce3+ center regulates the occurrence of radiative processes.
Lipophilicity Enhancing Substituents for ADDA Recognition Domains of DAAD·ADDA Heterodimers with Quadruple Hydrogen Bonds
Authors: Otte Philipp , Taubitz Jörg , Lüning Ulrich 
Published: February 2013

AbstractFour new ADDA receptors for DAAD·ADDA heterodimer formation by quadruple hydrogen bonds have been prepared. Their solubility has been improved by substitution with polyethylene glycol or branched alkyl chains. Three ADDA urea structures 8, 9, and 10 possess stabilizing intramolecular hydrogen bonds, in contrast to a related amide 7. The association constants Kass span several orders of magnitudes, with pyrimidinones 8 and 9 showing Kass values above 104 M–1.
Diagnostic Issues of Binge Eating in Eating Disorders
Authors: Birgegård Andreas , Clinton David , Norring Claes 
Published: February 2013

AbstractBackgroundDiagnostic and Statistical Manual of Mental Disorders (DSM) criteria for eating disorders (EDs) are problematic and due to be revised. One area of debate concerns binge eating.AimsWe investigated the impact of four aspects of binge eating (binge size, loss of control, frequency of objective and subjective binge eating) assessed by questionnaire and interview in adult ED patients on concurrent and follow‐up symptoms.MethodData came from two independent naturalistic Swedish ED databases (N = 2354, with 12‐month follow‐up; and N = 597, with 36‐month follow‐up).ResultsWe found fair concurrent validity of criteria assessed using questionnaire, but poor concurrent validity for interview assessment, and no prognostic validity regardless of assessment method.ConclusionsThe findings give support to some planned DSM‐5 revisions, but challenge the assumption of binge eating as a useful diagnostic criterion, as well as having implications for measurement. Copyright © 2013 John Wiley & Sons, Ltd and Eating Disorders Association.
THE NEONICOTINOID IMIDACLOPRID SHOWS HIGH CHRONIC TOXICITY TO MAYFLY NYMPHS
Authors: Roessink Ivo , Merga Lemessa B. , Zweers Hans J. , Van den Brink Paul J. 
Published: February 2013

Abstract

The present study evaluated the acute and chronic toxicity of imidacloprid to a range of freshwater arthropods. Mayfly and caddisfly species were most sensitive to short-term imidacloprid exposures (10 tests), whereas the mayflies showed by far the most sensitive response to long-term exposure of all seven arthropod species tested (28-d EC10 values of approximately 0.03 µg/L). The results indicated a high aquatic risk of chronic exposure of imidacloprid to mayflies. Environ. Toxicol. Chem. 2013;32:1096–1100. © 2013 SETAC
Aspect ratios and magma overpressures of non‐feeder dikes observed in the Miyake‐jima volcano (Japan), and fracture toughness of its upper part
Authors: Kusumoto Shigekazu , Geshi Nobuo , Gudmundsson Agust 
Published: February 2013

AbstractWe present a new method for estimating the length and maximum thickness (aperture) of a dike from the observed opening at one dike tip. We apply the method to 15 arrested non‐feeder dikes (where the upper tip is known, the lower tip unknown) in the caldera walls of Miyake‐jima, Japan, to estimate the length‐thickness ratio, as well as the magma overpressure and fracture toughness. The calculated length‐thickness ratio ranges from 61 to 246, with an average of 136. The ratios are low because the dikes are emplaced close to the surface in comparatively compliant (soft) rocks. Using these ratios and the appropriate elastic constants, the calculated magmatic overpressures of the dikes are between 2.3 and 8.9 MPa, and the stress intensity factors between 38 and 117 MPa m1/2. All these values are within the range of typical in situ estimates, supporting the validity of this new method.
Functional roles of glycogene and N‐glycan in multidrug resistance of human breast cancer cells
Authors: Ma Hongye , Miao Xiaoyan , Ma Qiuhong , Zheng Wenzhi , Zhou Huimin , Jia Li 
Published: February 2013

AbstractDrug resistance is a major problem in cancer chemotherapy. Aberrant glycosylation has been known to be associated with cancer chemoresistance. Aim of this work is to investigate the alterations of glycogene and N‐glycan involved in multidrug resistance (MDR) in human breast cancer cell lines. Using real‐time polymerase chain reaction (PCR) for quantification of glycogenes, fluorescein isothiocyanate (FITC)‐lectin binding for glycan profiling, and mass spectrometry for N‐glycan composition, the expression of glycogenes, glycan profiling, and N‐glycan composition differed between drug‐resistant MCF/ADR cells and the parental MCF‐7 line. Further analysis of the N‐glycan regulation by tunicamycin (TM) application or PNGase F treatment in MCF/ADR cells showed partial inhibition of the N‐glycan biosynthesis and increased sensitivity to chemotherapeutic drugs dramatically both in vitro and in vivo. Using an RNA interference strategy, we showed that the downregulation of MGAT5 in MCF/ADR cells could enhance the chemosensitivity to antitumor drugs both in vitro and in vivo. Conversely, a stable high expression of MGAT5 in MCF‐7 cells could increase resistance to chemotherapeutic drugs both in vitro and in vivo. In conclusion, the alterations of glycogene and N‐glycan in human breast cancer cells correlate with tumor sensitivity to chemotherapeutic drug and have significant implications for the development of new treatment strategies. © 2013 IUBMB Life, 65(5):409–422, 2013.
The diversity‐oriented synthesis of pteridines—achievements and potential for development
Authors: Suckling Colin J. 
Published: February 2013

AbstractThe importance of pteridines in the key cofactors, tetrahydrofolate and tetrahydrobiopterin, has encouraged the development of the chemistry and chemical biology of pteridines. In order to investigate the latter, versatile synthetic methods are required to prepare designed relatives of the natural cofactors for use as potential drugs or biological probes. Recently, diversity‐oriented synthesis has become a particularly powerful and versatile approach to the synthesis of pteridines and related heterocyclic compounds. This article describes and discusses available methods using radical, electrophilic, nucleophilic, and organometal mediated substitution reactions. © 2013 IUBMB Life 65(4):283–299, 2013.
The role of hair follicle nestin‐expressing stem cells during whisker sensory‐nerve growth in long‐term 3D culture
Authors: Mii Sumiyuki , Duong Jennifer , Tome Yasunori , Uchugonova Aisada , Liu Fang , Amoh Yasuyuki , Saito Norimitsu , Katsuoka Kensei , Hoffman Robert M. 
Published: February 2013

AbstractWe have previously reported that nestin‐expressing hair follicle stem cells can differentiate into neurons, Schwann cells, and other cell types. In the present study, vibrissa hair follicles, including their sensory nerve stump, were excised from transgenic mice in which the nestin promoter drives green fluorescent protein (ND‐GFP mice), and were placed in 3D histoculture supported by Gelfoam®. β‐III tubulin‐positive fibers, consisting of ND‐GFP‐expressing cells, extended up to 500 µm from the whisker nerve stump in histoculture. The growing fibers had growth cones on their tips expressing F‐actin. These findings indicate that β‐III tubulin‐positive fibers elongating from the whisker follicle sensory nerve stump were growing axons. The growing whisker sensory nerve was highly enriched in ND‐GFP cells which appeared to play a major role in its elongation and interaction with other nerves in 3D culture, including the sciatic nerve, the trigeminal nerve, and the trigeminal nerve ganglion. The results of the present report suggest a major function of the nestin‐expressing stem cells in the hair follicle is for growth of the follicle sensory nerve. J. Cell. Biochem. 114: 1674–1684, 2013. © 2013 Wiley Periodicals, Inc.
Dynamics of focal adhesions and reorganization of F‐actin in VEGF‐stimulated NSCs under varying differentiation states
Authors: Lyu Jingya , Hu Ya'nan , Xu Xiaojing , Zhang Huanxiang 
Published: February 2013

AbstractPrecise migration of neural stem/progenitor cells (NSCs) is crucially important for neurogenesis and repair in the nervous system. However, the detailed mechanisms are not clear. Our previous results showed that NSCs in varying differentiation states possess different migratory ability to vascular endothelial growth factor (VEGF). In this study, we demonstrate the different dynamics of focal adhesions (FAs) and reorganization of F‐actin in NSCs during spreading and migration stimulated by VEGF. We found that the migrating NSCs of 0.5 and 1 day differentiation possess more FAs at leading edge than cells of other states. Moreover, the phosphorylation of focal adhesion kinase (FAK) and paxillin in NSCs correlates closely with their differentiation states. VEGF promotes FA formation with broad lamellipodium generation at the leading edge in chemotaxing cells of 0, 0.5, and 1 day differentiation, but not in cells of 3 days differentiation. Furthermore, cells of 1 day differentiation show a maximal asymmetry of FAs between lamella and cell rear, orchestrating cell polarization and directional migration. Time‐lapse video analysis shows that the disassembly of FAs and the cell tail detachment in NSCs of 1 day differentiation are more rapid, along with the concurrent enlarged size of FAs at the leading edge, leading to the most effective chemotactic response to VEGF. Collectively, these results indicate that the dynamics of FAs and reorganization of F‐actin in NSCs that undergo directional migration correlate closely with their differentiation states, contributing to the different chemotactic responses of these cells to VEGF. J. Cell. Biochem. 114: 1744–1759, 2013. © 2013 Wiley Periodicals, Inc.
Histochemical comparison of the nonhost tomato with resistant wheat against Blumeria graminis f. sp. tritici
Authors: Hao Chongzhao , Chen Yanli , Zhang Bing , Li Yuefei , Zuo Hai , Qi Tuo , Ma Qing 
Published: February 2013

ABSTRACTThe nonhost interaction of tomato‐Blumeria graminis f. sp. tritici (Bgt) and resistant host interaction of wheat‐Bgt were compared histochemically. The percentage of appressorium formation had no significant difference on tomato and wheat leaves. Papilla formation occurred earlier and more on host wheat than on nonhost tomato leaves, while the incidence of hypersensitive cell death was much higher in the nonhost interaction. Whole‐cell H2O2 accumulation and hypersensitive cell death usually appeared in haustorium‐invaded wheat epidermal cells. In contrast, the vast majority of non‐haustorium epidermal cells were associated with H2O2 accumulation and hypersensitive cell death on tomato. Localized H2O2 accumulation and hypersensitive response were detected in effective papillae in both interactions. The peak percentage of haustorium formation was less than 7% in the nonhost interaction while reached 43% in the incompatible host interaction. These results indicate that hypersensitive cell death and papillae are likely to play an important role in preventing Bgt penetration and development on tomato and wheat leaves, both defense responses involving H2O2 accumulation. This study further implies that the nonhost and incompatible interactions share similar cytological mechanisms. Microsc. Res. Tech. 76:514–522, 2013. © 2013 Wiley Periodicals, Inc.
Effect of the root canal final rinse protocols on the debris and smear layer removal and on the push‐out strength of an epoxy‐based sealer
Authors: Aranda‐Garcia Arturo Javier , Kuga Milton Carlos , Vitorino Keli Regina , Chávez‐Andrade Gisselle Moraima , Hungaro Duarte Marco Antonio , Bonetti‐Filho Idomeo , Faria Gisele , Reis Só Marcus Vinicius 
Published: February 2013

ABSTRACTThe aim of the present study was to evaluate the efficacy of QMiX, SmearClear, and 17% EDTA for the debris and smear layer removal from the root canal and its effects on the push‐out bond strength of an epoxy‐based sealer by scanning electron microscopy (SEM). Forty extracted human canines (n = 10) were assigned to the following final rinse protocols: G1‐distilled water (control), G2–17% EDTA, G3‐SmearClear, and G4‐QMiX. The specimens were submitted to a SEM analysis to evaluate the presence of debris and smear layer, respectively, in the apical or cervical segments. In sequence, forty extracted human maxillary canines with the root canals instrumented were divided into four groups (n = 10) similar to the SEM analysis study. After the filling with AH Plus, the roots were transversally sectioned to obtain dentinal slices. The specimens were submitted to a push‐out bond strength test using an electromechanical testing machine. The statistical analysis for the SEM and push‐out bond strength studies were performed using the Kruskal–Wallis and Dunn tests (α = 5%). There was no difference among the G2, G3, and G4 efficacy in removing the debris and smear layer (P > 0.05). The efficacy of these groups was superior to the control group. The push‐out bond strength values of G2, G3, and G4 were superior to the control group. The ability to remove the debris and smear layer by SmearClear and QMiX was as effective as the 17% EDTA. The final rinse with these solutions promoted similar push‐out bond strength values. Microsc. Res. Tech. 76:533–537, 2013. © 2013 Wiley Periodicals, Inc.
Linking morphodynamic response with sediment mass balance on the Colorado River in Marble Canyon: Issues of scale, geomorphic setting, and sampling design
Authors: Grams Paul E. , Topping David J. , Schmidt John C. , Hazel Joseph E. , Kaplinski Matt 
Published: February 2013

AbstractMeasurements of morphologic change are often used to infer sediment mass balance. Such measurements may, however, result in gross errors when morphologic changes over short reaches are extrapolated to predict changes in sediment mass balance for long river segments. This issue is investigated by examination of morphologic change and sediment influx and efflux for a 100 km segment of the Colorado River in Grand Canyon, Arizona. For each of four monitoring intervals within a 7 year study period, the direction of sand‐storage response within short morphologic monitoring reaches was consistent with the flux‐based sand mass balance. Both budgeting methods indicate that sand storage was stable or increased during the 7 year period. Extrapolation of the morphologic measurements outside the monitoring reaches does not, however, provide a reasonable estimate of the magnitude of sand‐storage change for the 100 km study area. Extrapolation results in large errors, because there is large local variation in site behavior driven by interactions between the flow and local bed topography. During the same flow regime and reach‐average sediment supply, some locations accumulate sand while others evacuate sand. The interaction of local hydraulics with local channel geometry exerts more control on local morphodynamic response than sand supply over an encompassing river segment. Changes in the upstream supply of sand modify bed responses but typically do not completely offset the effect of local hydraulics. Thus, accurate sediment budgets for long river segments inferred from reach‐scale morphologic measurements must incorporate the effect of local hydraulics in a sampling design or avoid extrapolation altogether.
Sinonasal Disease in Polyostotic Fibrous Dysplasia and McCune–Albright Syndrome
Authors: DeKlotz Timothy R. , Kim Hung Jeffrey , Kelly Marilyn , Collins Michael T. 
Published: February 2013

AbstractObjectives/Hypothesis:To characterize the spectrum, symptoms, progression, and effects of endocrine dysfunction on sinonasal disease in polyostotic fibrous dysplasia (PFD) and McCune–Albright Syndrome (MAS).Study Design:Retrospective review.Methods:A prospectively followed cohort of subjects with PFD/MAS underwent a comprehensive evaluation that included otolaryngologic and endocrine evaluation, and imaging studies. Head and facial computed tomography scans were analyzed, and the degree of fibrous dysplasia (FD) was graded using a modified Lund–MacKay scale. Those followed for >4 years were analyzed for progression.Results:A total of 106 patients meeting inclusion criteria were identified with craniofacial FD. A majority (92%) demonstrated sinonasal involvement. There were significant positive correlations between the sinonasal FD scale score and chronic congestion, hyposmia, growth hormone excess, and hyperthyroidism (P < .05 for all). Significant correlations were not found for headache/facial pain or recurrent/chronic sinusitis. Thirty‐one subjects met the criteria for longitudinal analysis (follow‐up mean, 6.3 years; range, 4.4–9 years). Those who demonstrated disease progression were significantly younger than those who did not (mean age, 11 vs. 25 years). Progression after age of 13 years was uncommon (n = 3) and minimal. Concomitant endocrinopathy or bisphosphonate use did not have any significant effect on progression of disease.Conclusions:Sinonasal involvement of fibrous dysplasia in PFD/MAS is common. Symptoms are usually few and mild, and disease progression occurs primarily in young subjects. Concomitant endocrinopathy is associated with disease severity, but not progression.
Application of Electrospun Gas Diffusion Nanofibre‐membranes in the Determination of Dissolved Carbon Dioxide
Authors: Truong Yen B. , O'Bryan Yukie , McKelvie Ian D. , Kyratzis Ilias L. , Humphries William 
Published: February 2013

AbstractThis study demonstrates the first time use of electrospun nanofibrous hydrophobic membranes as gas diffusion (GD) membranes in flow injection analysis (FIA) for the determination of carbon dioxide. Different electrospun membranes are prepared from polyvinylidene fluoride (PVDF) and polystyrene (PS) polymers. To impart structural integrity for PVDF membrane, the addition of 1,4‐butanediol dimethacrylate (BDDMA) is used to form an interpenetrating network, while for PS membranes are spot welded with heat‐pressing at 80 °C. Heat pressed PS membranes are shown to have the best analytical sensitivity among other electrospun membranes and performed as well as the commercial PP membrane. A correlation is observed between the thickness of heat pressed PS membranes and analytical sensitivity. magnified image
Thermoresponsive Diblock Copolymer with Tunable Soluble–Insoluble and Soluble–Insoluble–Soluble Transitions
Authors: Han Xia , Zhang Xuxia , Yin Quanyi , Hu Jun , Liu Honglai , Hu Ying 
Published: February 2013

AbstractThe thermoresponsive transition behavior of a diblock copolymer consisting of poly(ethylene glycol) methyl ether (mPEG) and poly(N,N‐dimethylaminoethyl methacrylate) (PDMAEMA) in aqueous solutions has been investigated. With a specific composition, the copolymer showed a unique tunable phase transition from no response in acidic media to a soluble–insoluble (S‐I) transition in neutral media and an S‐I‐S transition in basic media either in the presence of salt or for salt‐free solutions. The S‐I‐S transition can be tuned over a wide temperature range even to an S‐I type transition just by adding salts. In addition, phase transitions can occur in both pure water and saline solution under practical conditions (30–80 °C), which makes them suitable for a broad range of applications.magnified image