bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of Clostridium tyrobutyricum Strain UC7086, Isolated from Grana Padano Cheese with Late-Blowing Defect
Authors: Bassi Daniela , Fontana Cecilia , Gazzola Simona , Pietta Ester , Puglisi Edoardo , Cappa Fabrizio , Cocconcelli Pier Sandro 
Published: August 2013

ABSTRACT

Clostridium tyrobutyricum
is considered the main agent of late-blowing defect in the production of hard cheese. Here, we described the draft genome sequences and annotation of
C. tyrobutyricum
strain UC7086, which was isolated from Grana Padano cheese with blowing defect, and
C. tyrobutyricum
DSM 2637 type strain in a comparative study.
Genome Sequence of an Epidemic Isolate of Mycobacterium abscessus subsp.
bolletii
from Rio de Janeiro, Brazil
Authors: Davidson Rebecca M. , Reynolds Paul R. , Farias-Hesson Eveline , Duarte Rafael Silva , Jackson Mary , Strong Michael 
Published: August 2013

ABSTRACT

Multiple isolates of
Mycobacterium abscessus
subsp.
bolletii
, collectively called BRA100, were associated with outbreaks of postsurgical skin infections across various regions of Brazil from 2003 to 2009. We announce the draft genome sequence of a newly sequenced BRA100 strain,
M. abscessus
subsp.
bolletii
CRM-0020, isolated from a patient in Rio de Janeiro, Brazil.
Genome Sequence of Lysinibacillus sphaericus Strain KCTC 3346
T
Authors: Jeong Haeyoung , Jeong Da-Eun , Sim Young Mi , Park Seung-Hwan , Choi Soo-Keun 
Published: August 2013

ABSTRACT

Lysinibacillus sphaericus
is a heterogeneous species that includes strains that produce mosquitocidal toxin proteins. Herein, we report the 4.56-Mb draft genome sequence of the nonpathogenic
L. sphaericus
strain KCTC 3346
T
, which provides clues for the phylogenetic reassessment of
L. sphaericus
species and an understanding of its physiological properties.
Comparative Genomic Analysis ofAcheta domesticusDensovirus Isolates from Different Outbreaks in Europe, North America, and Japan
Authors: Pham Hanh T. , Iwao Hajime , Szelei Jozsef , Li Yi , Liu Kaiyu , Bergoin Max , Tijssen Peter 
Published: August 2013

ABSTRACTThe first densovirus from a cricket,Acheta domesticusdensovirus (AdDNV) (Parvoviridae), was isolated in Europe in 1977 and has been studied previously. We compared seven additional AdDNV genomes isolated from 4 other European outbreaks, 2 major North American outbreaks, and a Japanese outbreak. Phylogenetic analysis suggested that the 2009 Japanese and North American outbreaks were not related.
Draft Genome Sequence of Winogradskyella psychrotolerans RS-3
T
, Isolated from the Marine Transect of Kongsfjorden, Ny-Ålesund, Svalbard, Arctic Ocean
Authors: Kumar Pinnaka Anil , Ara Sreenivas , Singh Aditya , Shivaji S. 
Published: August 2013

ABSTRACT

The 4.3-Mb genome of
Winogradskyella psychrotolerans
strain RS-3
T
, isolated from a sediment sample of a marine transect of Kongsfjorden, Ny-Ålesund, Svalbard, Arctic Ocean, is reported.
Draft Genome Sequence of Salmonella enterica subsp.
enterica
Serovar Manhattan Strain 111113, from an Outbreak of Human Infections in Northern Italy
Authors: Sassera Davide , Gaiarsa Stefano , Scaltriti Erika , Morganti Marina , Bandi Claudio , Casadei Gabriele , Pongolini Stefano 
Published: August 2013

ABSTRACT

We announce the draft genome sequence of
Salmonella enterica
subsp.
enterica
serovar Manhattan strain 111113, isolated from a patient during an outbreak in northern Italy. The genome, which was obtained with Illumina MiSeq technology, is composed of 21 contigs for a total of 4,684,342 bp, with a G+C content of 52.17%.
miR-153 Supports Colorectal Cancer Progression via Pleiotropic Effects That Enhance Invasion and Chemotherapeutic Resistance
Authors: Zhang Lei , Pickard Karen , Jenei Veronika , Bullock Marc D. , Bruce Amanda , Mitter Richard , Kelly Gavin , Paraskeva Christos , Strefford John , Primrose John , Thomas Gareth J. , Packham Graham , Mirnezami Alex H. 
Published: August 2013

Abstract
Although microRNAs (miRNA) have been broadly studied in cancer, comparatively less is understood about their role in progression. Here we report that miR-153 has a dual role during progression of colorectal cancer by enhancing cellular invasiveness and platinum-based chemotherapy resistance. miRNA profiling revealed that miR-153 was highly expressed in a cellular model of advanced stage colorectal cancer. Its upregulation was also noted in primary human colorectal cancer compared with normal colonic epithelium and in more advanced colorectal cancer stages compared with early stage disease. In colorectal cancer patients followed for 50 months, 21 of 30 patients with high levels of miR-153 had disease progression compared with others in this group with low levels of miR-153. Functional studies revealed that miR-153 upregulation increased colorectal cancer invasiveness and resistance to oxaliplatin and cisplatin both in vitro and in vivo. Mechanistic investigations indicated that miR-153 promoted invasiveness indirectly by inducing matrix metalloprotease enzyme 9 production, whereas drug resistance was mediated directly by inhibiting the Forkhead transcription factor Forkhead box O3a (FOXO3a). In support of the latter finding, we found that levels of miR-153 and FOXO3a were inversely correlated in matched human colorectal cancer specimens. Our findings establish key roles for miR-153 overexpression in colorectal cancer progression, rationalizing therapeutic strategies to target expression of this miRNA for colorectal cancer treatment. Cancer Res; 73(21); 6435–47. ©2013 AACR.
The Integrin Inhibitor Cilengitide Affects Meningioma Cell Motility and Invasion
Authors: Wilisch-Neumann Annette , Kliese Nadine , Pachow Doreen , Schneider Thomas , Warnke Jan-Peter , Braunsdorf Werner EK , Böhmer Frank-Dietmar , Hass Peter , Pasemann Diana , Helbing Cornelia , Kirches Elmar , Mawrin Christian 
Published: August 2013

Abstract
Purpose: Meningiomas are frequent intracranial or spinal neoplasms, which recur frequently and can show aggressive clinical behaviour. We elucidated the impact of the integrin inhibitor cilengitide on migration, proliferation, and radiosensitization of meningioma cells.
Experimental Design: We analyzed integrin expression in tissue microarrays of human meningiomas and the antimeningioma properties of cilengitide in cell cultures, subcutaneous and intracranial nude mouse models by measuring tumor volumes and survival times.
Results: αvβ5 was the predominantly expressed integrin heterodimer in meningiomas, whereas αvβ3 was mainly detected in tumor blood vessels. Application of up to 100 μg/mL cilengitide resulted in only mildly reduced proliferation/survival of meningioma cell lines. Effects on cell survival could be enhanced by irradiation. One μg/mL cilengitide was sufficient to significantly inhibit meningioma cell migration and invasion in vitro. A daily dosage of 75 mg/kg did neither affect tumor volumes nor overall survival (P = 0.813, log-rank test), but suppressed brain invasion in a significant fraction of treated animals. A combination of 75 mg/kg cilengitide daily and irradiation (2 × 5 Gy) led to a 67% reduction of MRI-estimated tumor volumes in the intracranial model (P &lt; 0.01), whereas the corresponding reduction reached by irradiation alone was only 55% (P &lt; 0.05).
Conclusions: These data show that a monotherapy with cilengitide is not likely to achieve major responses in rapidly growing malignant meningiomas, although brain invasion may be reduced because of the strong antimigratory properties of the drug. The combination with radiotherapy warrants further attention. Clin Cancer Res; 19(19); 5402–12. ©2013 AACR.
Evaluation of Rural Stroke Services
Authors: Cadilhac Dominique A. , Purvis Tara , Kilkenny Monique F. , Longworth Mark , Mohr Katherine , Pollack Michael , Levi Christopher R. 
Published: August 2013

Background and Purpose—
The quality of hospital care for stroke varies, particularly in rural areas. In 2007, funding to improve stroke care became available as part of the Rural Stroke Project (RSP) in New South Wales (Australia). The RSP included the employment of clinical coordinators to establish stroke units or pathways and protocols, and more clinical staff. We aimed to describe the effectiveness of RSP in improving stroke care and patient outcomes.

Methods—
A historical control cohort design was used. Clinical practice and outcomes at 8 hospitals were compared using 2 medical record reviews of 100 consecutive ischemic or intracerebral hemorrhage patients ≥12 months before RSP and 3 to 6 months after RSP was implemented. Descriptive statistics and multivariable analyses of patient outcomes are presented.

Results—

Sample: pre-RSP n=750; mean age 74 (SD, 13) years; women 50% and post-RSP n=730; mean age 74 (SD, 13) years; women 46%. Many improvements in stroke care were found after RSP: access to stroke units (pre 0%; post 58%,
P
<0.001); use of aspirin within 24 hours of ischemic stroke (pre 59%; post 71%,
P
<0.001); use of care plans (pre 15%; post 63%,
P
<0.001); and allied health assessments within 48 hours (pre 65%; post 82%
P
<0.001). After implementation of the RSP, patients directly admitted to an RSP hospital were 89% more likely to be discharged home (adjusted odds ratio, 1.89; 95% confidence interval, 1.34–2.66).

Conclusions—
Investment in clinical coordinators who implemented organizational change, together with increased clinician resources, effectively improved stroke care in rural hospitals, resulting in more patients being discharged home.
The Contribution of Extratropical Waves to the MJO Wind Field
Authors: Adames Ángel F. , Patoux Jérôme , Foster Ralph C. 
Published: August 2013

Abstract
A method for capturing the different dynamical components of the Madden–Julian oscillation (MJO) is presented. The tropical wind field is partitioned into three components using free-space Green’s functions: 1) a nondivergent component, 2) an irrotational component, and 3) a background or environmental flow that is interpreted as the influence on the tropical flow due to vorticity and divergence elements outside of the tropical region. The analyses performed in this study show that this background flow is partly determined by a train of extratropical waves. Space–time power spectra for each flow component are calculated. The strongest signal in the nondivergent wind spectrum corresponds to equatorial Rossby, mixed Rossby–gravity, and easterly waves. The strongest signal in the irrotational winds corresponds to Kelvin and inertia–gravity modes. The strongest signal in the power spectrum of the background flow corresponds to the wave band of extratropical Rossby waves. Furthermore, a coherence analysis reveals that the background flow has the highest coherence with geopotential height variations in the latitude bands from 30° to 45° in both the Northern and Southern Hemispheres.
The flow partitions are further studied through a composite analysis based on the Wheeler–Hendon MJO index. Anomalies in the background flow are strongest in the western and central Pacific, possess an equivalent barotropic structure, and show an eastward propagation. By contrast, the irrotational and nondivergent winds possess a first-mode baroclinic structure. An oscillation in the zonally averaged background flow with the MJO phases is observed but contributes little to tropical angular momentum when compared to the nondivergent flow.
The correlates of urinary albumin to creatinine ratio (ACR) in a high risk Australian aboriginal community
Authors: Wang Zaimin , Hoy Wendy E , Wang Zhiqiang 
Published: August 2013

Abstract

Background
Albuminuria marks renal disease and cardiovascular risk. It was estimated to contribute 75% of the risk of all-cause natural death in one Aboriginal group. The urine albumin/creatinine ratio (ACR) is commonly used as an index of albuminuria. This study aims to examine the associations between demographic factors, anthropometric index, blood pressure, lipid-protein measurements and other biomarkers and albuminuria in a cross-sectional study in a high-risk Australian Aboriginal population. The models will be evaluated for albuminuria at or above the microalbuminuria threshold, and at or above the “overt albuminuria” threshold with the potential to distinguish associations they have in common and those that differ.

Methods
This was a cross-sectional study of 598 adults aged 18–76 years. All participants were grouped into quartiles by age. Logistic regression models were used to explore the correlates of ACR categories.

Results
The significant correlates were systolic blood pressure (SBP), C-reactive protein (CRP), uric acid, diabetes, gamma-glutamyl transferase (GGT) (marginally significant, p = 0.054) and serum albumin (negative association) for ACR 17+ (mg/g) for men and 25+ for women. Independent correlates were SBP, uric acid, diabetes, total cholesterol, alanine amino transferase (ALT), Cystatin C and serum albumin (negative association) for overt albuminuria; and SBP, CRP and serum albumin only for microalbuminuria.

Conclusions
This is the most detailed modelling of pathologic albuminuria in this setting to date. The somewhat variable association with risk factors suggests that microalbuminuria and overt albuminuria might reflect different as well as shared phenomena.
More of the same or a change of scenery: an observational study of variety and frequency of physical activity in British children
Authors: Brooke Hannah L , Corder Kirsten , Griffin Simon J , Ekelund Ulf , van Sluijs Esther MF 
Published: August 2013

Abstract

Background
Physical activity is important for children’s health, but successful physical activity promotion is challenging. Whether performing many different types of activities (Variety) is associated with higher physical activity independent of the number of activity sessions (Frequency) is unknown, but this information could inform physical activity promotion and public health strategies in children.

Methods
In the SPEEDY study we measured moderate-to-vigorous intensity physical activity (MVPA; ≥2000 counts/minute) over 7 days using GT1M Actigraph accelerometers in 1700 children from Norfolk, UK (56% girls, Mean ± SD 10.3 ± 0.3 years-old). Children reported participation in 28 leisure-time activities over the previous 7 days. Sex differences in activity participation were assessed using multilevel logistic regression, clustered by school. Associations of log-transformed MVPA with z-score-Variety (number of different activities/week) and z-score-Frequency (sum of all activity sessions/week) were examined using multilevel linear regression, adjusted for age, sex, parental education and age-standardised BMI.

Results
Children’s activity participation often reflected gender stereotypes. Mean ± SD Variety was 10.8 ± 5.0 activities/week, and Frequency was 24.2±15.0 sessions/week. In separate models lnMVPA had similar strength, positive associations with z-score-Variety and z-score-Frequency (Exp β(95% CI); Variety 1.04(1.02-1.06), Frequency 1.04(1.02-1.06)). lnMVPA was not associated with z-score-Variety independent of z-score-Frequency (Variety 1.01(0.98-1.04), Frequency 1.03(1.00-1.06)).

Conclusions
Future physical activity interventions and public health strategies could allow for gender specific activity preferences and could target both Variety and Frequency of activities participated in by children.
Knowledge and attitude of ICU nurses, students and patients towards the Austrian organ donation law
Authors: Stadlbauer Vanessa , Steiner Peter , Schweiger Martin , Sereinigg Michael , Tscheliessnigg Karl-Heinz , Freidl Wolfgang , Stiegler Philipp 
Published: August 2013

Abstract

Background
A survey on the knowledge and attitudes towards the Austrian organ donation legislation (an opt-out solution) of selected groups of the Austrian population taking into account factors such as age, gender, level of education, affiliation to healthcare professions and health related studies was conducted.

Methods
An online survey among 3 target groups (ICU nurses, health science students and non health science students) was performed and results were compared to the answers from transplantation patients to a paper questionnaire. A total of 8415 persons were asked to participate in the survey and 2025 (24%) persons correctly completed the questionnaire. 1945 online responses (ICU nurses n = 185; students of health sciences n = 1277; students of non-health science related courses n = 483) were analysed and data were compared to 80 manually filled-in responses from patients from a previous study.

Results
84% of participants state that they know the Austrian organ donation legislation; this percentage varies significantly (p < 0.05) within the target groups and is influenced by demographic variables of the participants. 74% think that the law is good and 79% do not favour a change. Opinions and attitudes towards the legal situation are positively influenced by the affiliation to healthcare professions and health-related fields of study. Interviewed persons who were aware of the legislation before the survey had a more positive attitude towards the existing legislation (77% versus 74%, p < 0.05).

Conclusions
The information level on Austrian organ donation legislation is high. ICU nurses and those who did not know the law before were most critical towards the existing legislation. Therefore education to increase knowledge in the general population and goal-oriented efforts to increase awareness in the target groups should be emphasized.
Circulating concentrations of GLP-1 are associated with coronary atherosclerosis in humans
Authors: Piotrowski Katja , Becker Melanie , Zugwurst Julia , Biller-Friedmann Ingeborg , Spoettl Gerald , Greif Martin , Leber Alexander W , Becker Alexander , Laubender Rüdiger P , Lebherz Corinna , Goeke Burkhard , Marx Nikolaus , Parhofer Klaus G , Lehrke Michael 
Published: August 2013

Abstract

Background
GLP-1 is an incretine hormone which gets secreted from intestinal L-cells in response to nutritional stimuli leading to pancreatic insulin secretion and suppression of glucagon release. GLP-1 further inhibits gastric motility and reduces appetite which in conjunction improves postprandial glucose metabolism. Additional vasoprotective effects have been described for GLP-1 in experimental models. Despite these vasoprotective actions, associations between endogenous levels of GLP-1 and cardiovascular disease have yet not been investigated in humans which was the aim of the present study.

Methods
GLP-1 serum levels were assessed in a cohort of 303 patients receiving coronary CT-angiography due to typical or atypical chest pain.

Results
GLP-1 was found to be positively associated with total coronary plaque burden in a fully adjusted model containing age, sex, BMI, hypertension, diabetes mellitus, smoking, triglycerides, LDL-C (low density lipoprotein cholesterol), hsCRP (high-sensitive C-reactive protein), and eGFR (estimated glomerular filtration rate) (OR: 2.53 (95% CI: 1.12 – 6.08; p = 0.03).

Conclusion
Circulating GLP-1 was found to be positivity associated with coronary atherosclerosis in humans. The clinical relevance of this observation needs further investigations.
Psychological investigation in patients with polycystic ovary syndrome
Authors: Bazarganipour Fatemeh , Ziaei Saeide , Montazeri Ali , Foroozanfard Fatemeh , Kazemnejad Anoshirvan , Faghihzadeh Soghrat 
Published: August 2013

Abstract

Background
The aim of the present study was to estimate the prevalence of mood disorders and examine a range of predictors for psychological well-being among Iranian women with polycystic ovary syndrome (PCOS).

Methods
A cross-sectional study was undertaken to ascertain the factors related to psychological distress in PCOS patients in Kashan, Iran. Psychological distress was measured using the Hospital Anxiety and Depression Scale (HADS). In addition we assessed quality of life using the Short Form Health Survey (SF-36). Socio-demographic details and clinical information of PCOS including obesity (body mass index), excessive body hair (hirsutism score), acne, menstrual cycle disturbances, infertility and endocrine profile also were recorded for each patient.

Results
In all 300 women with PCOS were entered into the study. Of these 32% (n =96) showed elevated HADS anxiety while depression was high in 5% (n =15). Quality of life was significantly impaired in women with anxiety (P <0.05), and depression (P <0.001) and in particular, in women with coexistence anxiety and depression (P <0.001) compared with unaffected participants. Compared with the non-depressed PCOS patients, the depressed women had significantly higher menstrual irregularities (P = 0.008). Moreover, we found significant difference in FAI level between the depressed and non depressed (p = 0.05), the anxious and non anxious patients (p < 0.001) compare to non affected PCOS women.

Conclusions
The high prevalence of depression and anxiety in this population suggests that initial evaluation of all women with PCOS should also include assessment of mental health disorders. The clinician should further pay attention to background of their patients especially in view of the factors influencing psychological well-being.
Highly stable carbon nanotube field emitters on small metal tips against electrical arcing
Authors: Ha Jun Mok , Kim Hyun Jin , Raza Hamid Saeed , Cho Sung Oh 
Published: August 2013

Abstract
Carbon nanotube (CNT) field emitters that exhibit extremely high stability against high-voltage arcing have been demonstrated. The CNT emitters were fabricated on a sharp copper tip substrate that produces a high electric field. A metal mixture composed of silver, copper, and indium micro- and nanoparticles was used as a binder to attach CNTs to the substrate. Due to the strong adhesion of the metal mixture, CNTs were not detached from the substrate even after many intense arcing events. Through electrical conditioning of the as-prepared CNT emitters, vertically standing CNTs with almost the same heights were formed on the substrate surface and most of loosely bound impurities were removed from the substrate. Consequently, no arcing was observed during the normal operation of the CNT emitters and the emission current remained constant even after intentionally inducing arcing at current densities up to 70 mA/cm2.
Mate preference in the painted goby: the influence of visual and acoustic courtship signals
Authors: Amorim Maria Clara P. , Nunes da Ponte Ana , Caiano Manuel , Pedroso Silvia S. , Pereira Ricardo , Fonseca Paulo J. 
Published: August 2013

Summary
We tested the hypothesis that females of a small vocal marine fish with exclusive paternal care, the painted goby, prefer high parental-quality mates such as large or high condition males. We tested the effect of male body size and male visual and acoustic courtship behaviour (playback experiments) on female mating preferences, by measuring time spent near one of a two-choice stimuli. Females did not show preference for male size but preferred males that showed higher levels of courtship, a trait know to advertise condition (fat reserves). Also, time spent near the preferred male depended on male courtship effort. Playback experiments showed that when sound was combined with visual stimuli (a male confined in a small aquarium placed near each speaker), females spent more time near the male associated with courtship sound than with the control male (associated with white noise or silence). Although male visual courtship effort also affected female preference in the pre-playback period, this effect decreased during playback and disappeared in the post-playback period. Courtship sound stimuli alone did not elicit female preference in relation to a control. Taken together, the results suggest that visual and mainly acoustic courtship displays are subject to mate preference and may advertise parental quality in this species. Our results indicate that visual and acoustic signals interplay in a complex fashion and highlight the need to examine how different sensory modalities affect mating preferences in fish and other vertebrates.