bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of polyphenols extracted from Keemun black tea on CYP450s activity and molecular mechanisms
Authors: Zuo Dan , Lv Le , Ren Hong , Sun Haiyan 
Published: July 2024

AbstractKeemun black tea (KBT) is a luxurious traditional tea in China that has been commonly consumed because of its superior aroma and special taste. However, the risks remain unknown when KBT is used concomitantly with other drugs or food products. Therefore, we aimed to explore the effect of the tea polyphenols from KBT on the protein and mRNA levels of CYP450 and related mechanisms. The extraction of tea polyphenols from KBT and the content and component analysis of polyphenols were performed. A total of 24 female C57BL/6J mice were given tea polyphenols (0, 75, 150, 300 mg/kg) for 7 days, respectively. Liver tissues were collected 2 h after the last administration. The expression of Cyp3a11, Cyp1a2, Cyp2e1, Cyp2c37, and PXR mRNA was detected by real‐time PCR, and the expression of Cyp3a11, Cyp1a2, Cyp2e1, Cyp2c37, and PXR protein was detected by Western blotting. A transient co‐transfection reporter gene assay on HepG2 cells was also used to verify the role of PXR in regulating CYP3A4 expression. Our results showed that tea polyphenols from KBT significantly induced the expression of CYP 3A11 and PXR in general, inhibited the expression of Cyp1a2 and Cyp2e1 in general, and significantly inhibited the mRNA expression of Cyp2c37 but induced its protein expression. The reporter gene‐transfected cells demonstrated that tea polyphenols could enhance the PXR‐mediated transactivation of the CYP3A4 promoter via rifampicin‐induction. Meanwhile, tea polyphenols could significantly accelerate CYP3A11/3A4 expression by activating the PXR‐CYP3A4 pathway. In conclusion, KBT polyphenols could significantly affect the expression of various subtypes of the Cyp450 enzyme in mice livers via the PXR‐CYP450 pathway, suggesting that metabolism‐based interactions can occur when they are used in combination with medicines.
Optimizing Genetic Algorithm With Momentum Strategy for Technical Trading Rules: Evidence From Futures Markets
Authors: Li Shihan , Li Shuyao , Liu Qingfu , Tse Yiuman 
Published: July 2024

ABSTRACTThis paper introduces an innovative genetically optimized dynamic composite strategy for achieving profitability in futures markets. Utilizing daily data from 35 actively traded futures contracts (1984–2022), we highlight the potential advantages of integrating the momentum effect into dynamic moving average strategies. This enhancement can boost the strategy's capability to capture and capitalize on market trends, ensuring consistent and stable returns. The developed dynamically composite technical trading strategy aspires to be a valuable reference for investors and the finance academic community, contributing to advancements in the field.
Non‐classical measurement error in instrumental variables estimation: An application to the medical care costs of obesity
Authors: Biener Adam I. , Meyerhoefer Chad , Cawley John 
Published: July 2024

AbstractEstimates of the impact of body mass index and obesity on health and labor market outcomes often use instrumental variables estimation (IV) to mitigate bias due to endogeneity. When these studies rely on survey data that include self‐ or proxy‐reported height and weight, there is non‐classical measurement error due to the tendency of individuals to under‐report their own weight. Mean reverting errors in weight do not cause IV to be asymptotically biased per se, but may result in bias if instruments are correlated with additive error in weight. We demonstrate the conditions under which IV is biased when there is non‐classical measurement error and derive bounds for this bias conditional on instrument strength and the severity of mean‐reverting error. We show that improvements in instrument relevance alone cannot eliminate IV bias, but reducing the correlation between weight and reporting error mitigates the bias. A solution we consider is regression calibration (RC) of endogenous variables with external validation data. In simulations, we find IV estimation paired with RC can produce consistent estimates when correctly specified. Even when RC fails to match the covariance structure of reporting error, there is still a reduction in asymptotic bias.
Effectiveness of adalimumab in severe ulcerative colitis: A systematic review and a meta‐analysis
Authors: Azadbakht Saleh , Seighali Masomeh , Azadbakht Salehe , Azadbakht Morteza 
Published: July 2024

AbstractBackground and AimUlcerative colitis (UC) causes chronic inflammation in the digestive tract, leading to abdominal pain and diarrhea. Adalimumab, a monoclonal antibody, is used to treat moderate to severe cases. This review and meta‐analysis evaluated adalimumab's effectiveness for severe UC, considering patient age, disease duration, and gender.MethodsThis study was designed as a systematic review and a meta‐analysis. Articles were searched in PubMed, Scopus, and Web of Science databases based on the keywords of adalimumab and UC. The titles, the abstracts, and, if necessary, the full texts of the articles were read. Then for further review, the full texts of the related articles were carefully examined, and the final articles were selected. Seventy‐eight articles were searched based on the keywords, and after reading the articles, 50 articles were related to the topic of the dissertation. The 50 articles were evaluated critically based on a checklist prepared by a statistical consultant and four articles with a score above 70% were selected. In the four articles, the main indicators of the effectiveness of adalimumab, including mucosal healing, clinical remission, and clinical response, were evaluated.ResultsThe effectiveness of adalimumab on the mucosal healing index was 75.40%, the clinical remission index was 70.79%, and the clinical response index was 83.02%, based on different doses and treatment durations in the study. In the four meta‐analysis studies on adalimumab's effectiveness, 1613 UC patients were treated with varying doses over 8 and 52 weeks. Based on a meta‐analysis over 8 and 52 weeks for treating moderate to severe UC, adalimumab's effectiveness was 70%−83%. The highest effectiveness, based on three main indices, was with a 40 mg dose over 52 weeks.ConclusionAccording to the meta‐analysis, the effectiveness of adalimumab for treating moderate to severe UC over 8 and 52 weeks was 70%−83%. The highest effectiveness, based on three main indices, was with a 40 mg dose over 52 weeks.
Early neutrophil gelatinase‐associated lipocalin (NGAL) measurement could rule out future acute kidney injury in patients with acute coronary syndrome—Prospective observational study
Authors: Magnusson Nils Erik , Frydman Shir , Freund Ophir , Zornizki Lior , Banai Shmuel , Shacham Yacov 
Published: July 2024

AbstractBackground and AimsThe diagnosis of acute kidney injury (AKI) is of importance among patients with ST segment elevation (STEMI) undergoing primary coronary intervention (PCI). It is often delayed given the need in serial measurements of creatinine or other serum markers. Neutrophil gelatinase‐associated lipocalin (NGAL) is a proven marker for AKI, although its role as an early predictor in this setting was scarcely addressed before and was the aim of our study.MethodsProspective observational study including 133 patients with STEMI treated with PCI. Plasma NGAL was drawn immediately before PCI (NGAL‐0) and 24 h after (NGAL‐24). Similar analysis of C‐reactive protein (CRP) was performed for additional comparison.ResultsMean age was 62 ± 13 years, 78% were men, and 20 (15%) developed AKI after admission. Patients with AKI after admission demonstrated higher levels of NGAL‐0 (164 vs. 95 ng/mL; p < 0.001) and NGAL‐24 (142 vs. 93 ng/mL; p < 0.001). Levels of NGAL‐0 and NGAL‐24 were similar within the AKI and non‐AKI groups. Using ROC curve analysis, NGAL‐0 had best predictive ability for AKI development (AUC 0.841, 95% CI 0.80–0.96), compared with NGAL‐24 (0.783, 95% CI 0.74–0.85), CRP‐0 (0.701, 95% CI 0.58–0.83), and CRP‐24 (0.781, 95% CI 0.66–0.90). The optimal NGAL‐0 cutoff for AKI prediction was 125 ng/mL, with 70% sensitivity, 84% specificity, and 94% negative predictive value.ConclusionsAmong STEMI patients, NGAL measurement upon admission are associated with AKI and may serve as a reliable marker for early AKI detection. Future studies may direct risk stratification using this single test can direct personalized evaluations during the admission, and focused interventions to prevent AKI.
Assessing the true prevalence of endometriosis: A narrative review of literature data
Authors: Harder Carolin , Velho Renata Voltolini , Brandes Iris , Sehouli Jalid , Mechsner Sylvia 
Published: July 2024

AbstractEndometriosis is a gynecologic condition often described as the “chameleon of gynecology” because of its elusive symptoms. The World Health Organization acknowledges its severe impact on quality of life due to pain, fatigue, depression, and infertility. There is limited literature on the prevalence of endometriosis, knowledge of which is essential for effective prevention, treatment strategies, and funding. We aim to structure and present the published data on endometriosis prevalence, synthesizing the discrepancy between health insurance and clinical data. PubMed, Cochrane CENTRAL, and LIVIVO were researched for peer‐reviewed articles published between January 2000 and July 2023 on women of reproductive age. Health insurance companies argue that few women (~1%) are affected by endometriosis. Interestingly, the prevalence of clinical data studies (6.8%), population‐based surveys/self‐reported studies (6.6%), and symptomatic patient data (21%) revealed a different picture. Based on the data gathered, a multi‐layered prevalence model has been proposed to illustrate the large discrepancy in the prevalence numbers, leading to the disease being underestimated and underfunded. This variability may be influenced by the heterogeneity in designs and the analyzed data, and clinical complexity. In summary, this narrative review reveals that the prevalence of endometriosis is higher than health insurance and other stakeholders might have previously assumed.
The effect of opting‐in versus opting‐out forming on the rate of reported variants of questionable significance in prenatal microarray
Authors: Haddad‐Halloun Jumana , Wexler Ava , Echar Moran , Harari‐Shaham Amalia , Polager‐Modan Shirley , Zaatry Rawan , Maayouf Rasha , Assadi Maisa , Krivoruk Olga , Peleg Amir , Sagi‐Dain Lena 
Published: July 2024

AbstractObjectiveTo examine the effect of patient‐selected opt‐in versus opt‐out option on the rate of reported variants of uncertain clinical significance (VOUS) and high‐frequency low‐penetrant (HFLP) findings in prenatal microarray testing.MethodsA standard microarray consent form in Israel includes a requirement to note patient choice to be or not to be informed about the presence of VOUS and HFLP variants. The original form was designed as an opting‐out method, in which the women had to actively mark if they did not want to be informed about questionable findings. In the authors' Genetic Institute, the form was changed for an opting‐in option in October 2019. In this study we have compared the rates of reported VOUS and HFLP variants between the opt‐in and opt‐out periods.ResultsOf the 1014 prenatal CMA tests, 590 (58.2%) were performed in the opt‐out period. A significant decrease in the rate of women requesting to be informed of VOUS findings was noted (66.8% in opt‐out period vs 34.0% in opt‐in period), yielding a relative risk (RR) of 0.46 (95% confidence interval [CI] 0.39–0.53). Rate of women preferring to be informed of HFLP variants decreased from 75.3% to 48.1% (RR 0.52, 95% CI 0.45–0.60).DiscussionWe present a simple and effective method to decrease the rate of reported findings of questionable significance in the prenatal setting. These results are important not only for microarray results, but also for next‐generation sequencing techniques, such as whole exome or genome sequencing.
Approach to managing chronic vomiting in dogs
Authors: Rogers‐Smith Emma 
Published: July 2024

Background: Chronic vomiting is a common and, at times, frustrating clinical presentation in our canine patients. Vomiting is considered ‘chronic’ in cases that have gone on for over three to four weeks and the clinical presentation in these cases can vary widely. Given that the list of potential differentials is extensive, a careful and methodical approach to these cases is paramount to successful management.Aim of the article: This article considers an approach to achieving a diagnosis in the chronically vomiting patient, providing readers with the necessary diagnostic toolkit. It also discusses appropriate management strategies to consider in these cases.
CircCOL5A1 is involved in proliferation, invasion, and inhibition of ferroptosis of colorectal cancer cells via miR‐1287‐5p/SLC7A11
Authors: Wang Junwei , Zhang Zili , Zhuang Jianbin , Kang Di , Song Weiliang 
Published: July 2024

AbstractColorectal cancer (CRC) is the leading cause of cancer‐related death globally. Circular RNA circCOL5A1 plays an oncogene function in a variety of tumors. However, the function of circCOL5A1 in CRC is still unknown. Here, we aimed to elucidate the function and mechanism of circCOL5A1 in CRC. The correlation between circCOL5A1 and CRC clinicopathological was assessed through chi‐square. The relevance between circCOL5A1 and CRC patient survival time was evaluated by Kaplan–Meier analysis. The expressions of circCOL5A1 in CRC were determined via quantitative real‐time PCR. The function of circCOL5A1 in CRC was analyzed with Cell Counting Kit‐8, EdU assay, Transwell, detection of reactive oxygen species and Fe2+ levels, and Western blot analysis. Moreover, the mechanism of circCOL5A1 was determined by dual‐luciferase reporter assay, RNA immunoprecipitation, and RNA pull‐down. Finally, the role of circCOL5A1 in vivo was elucidated through a mouse xenograft model, hematoxylin–eosin staining, and immunohistochemistry. CircCOL5A1 expression was increased in CRC, and increased circCOL5A1 levels were related to TNM stage, lymph node metastasis, distant metastasis, and tumor differentiation in CRC patients, and CRC patients with high circCOL5A1 levels had a low overall survival rate. For the circCOL5A1 function in CRC, we found that circCOL5A1 knockdown weakened CRC cell proliferation and invasion, and enhanced cell ferroptosis. For the circCOL5A1 mechanism in CRC, we further confirmed that circCOL5A1 bound to miR‐1287‐5p, miR‐1287‐5p bound to SLC7A11. SLC7A11 was negatively interrelated to miR‐1287‐5p and was positively interrelated to circCOL5A1 in CRC tissues. Furthermore, interfering circCOL5A1 decreased SLC7A11 expression, and this trend was abolished through miR‐1287‐5p cotransfection. Rescue assays further demonstrated that circCOL5A1 knockdown alleviated CRC cell malignant phenotype via miR‐1287‐5p/SLC7A11. Moreover, interference with circCOL5A1 reduced CRC growth in vivo. CircCOL5A1 functioned as an oncogene in CRC via miR‐1287‐5p/SLC7A11.
Renal and multisystem effectiveness of 3.9 years of migalastat in a global real‐world cohort: Results from the followME Fabry Pathfinders registry
Authors: Hughes Derralynn A. , Sunder‐Plassmann Gere , Jovanovic Ana , Brand Eva , West Michael L. , Bichet Daniel G. , Pisani Antonio , Nowak Albina , Torra Roser , Khan Aneal , Azevedo Olga , Lehman Anna , Linhart Aleš , Rutecki Jasmine , Giuliano Joseph D. , Krusinska Eva , Nordbeck Peter 
Published: July 2024

AbstractFabry disease is a progressive, X‐linked lysosomal disorder caused by reduced or absent α‐galactosidase A activity due to GLA variants. The effects of migalastat were examined in a cohort of 125 Fabry patients with migalastat‐amenable GLA variants in the followME Pathfinders registry (EUPAS20599), an ongoing, prospective, patient‐focused registry evaluating outcomes for current Fabry disease treatments. We report annualised estimated glomerular filtration rate (eGFR) and Fabry‐associated clinical events (FACEs) in a cohort of patients who had received ≥3 years of migalastat treatment in a real‐world setting. As of August 2022, 125 patients (60% male) had a mean migalastat exposure of 3.9 years. At enrolment, median age was 58 years (males, 57; females, 60) with a mean eGFR of 83.7 mL/min/1.73 m2 (n = 122; males, 83.7; females, 83.8) and a median left ventricular mass index of 115.1 g/m2 (n = 61; males, 131.2; females, 98.0). Mean (95% confidence interval) eGFR annualised rate of change in the overall cohort (n = 116) was −0.9 (−10.8, 9.9) mL/min/1.73 m2/year with a similar rate of change observed across patients with varying levels of kidney function at enrolment. Despite population age and baseline morbidity, 80% of patients did not experience a FACE during the mean 3.9 years of migalastat exposure. The incidence of renal, cardiac, and cerebrovascular events was 2.0, 83.2, and 4.1 events per 1000 patient‐years, respectively. These data support a role of migalastat in preserving renal function and multisystem effectiveness during ≥3 years of migalastat treatment in this real‐world Fabry population.
Investigating social communication in mice: A two‐intruders test approach
Authors: Morozova Maryana V. , Boldyreva Lidiya V. , Borisova Maria A. , Kozhevnikova Elena N. 
Published: July 2024

AbstractUnderstanding the complex dynamics of social communication behaviors, such as exploration, communication, courtship, mating, and aggression in animal models, is crucial to reveal key neural and hormonal mechanisms underlying these behaviors. The two‐intruders test is designed to investigate residents' behavior toward both male and female intruders within the home cage of the test male. During this test imitating natural conditions, several aspects of social interaction were investigated: Exploration, courtship, mating, and aggressive behavior. As mating and aggression involve overlapping neural circuits, the behavioral setup testing both behaviors is best at reflecting their competitive nature. Our findings demonstrate that resident male mice exhibit strong preference to communicate with a female intruder, which correlates with baseline testosterone levels of test males. Relevant female preference in the two‐intruders test was also found in BALB/c males. Behavioral breakdown revealed the anogenital sniffing as a key behavioral feature that discriminates resident male behavior toward intruders of different sex. Furthermore, resident male interaction with female intruder was accompanied by neuronal activation in the ventromedial hypothalamus. We demonstrate that odor recognition underlies preference toward females in male residents, as experimental anosmia reduced communication with a female intruder. We conclude the two‐intruders test setup to be a useful tool to study the neurological basis of social communication in animal models, which provides detailed analysis of various aspects of the laboratory animals’ social behavior in the most natural conditions.
Factors Influencing the Visualization of Fallopian Tubes in Hysterosalpingo‐Contrast Sonography (HyCoSy)—The Value of Multimodal HyCoSy in Visualizing the Fallopian Tubes
Authors: Li Yan , Chen Xiaoyi , Wang Qin , Wang Jingping 
Published: July 2024

BackgroundFour‐dimensional hysterosalpingo‐contrast sonography (4D‐HyCoSy) can non‐invasively evaluate the patency of the fallopian tubes and is increasingly used in clinical practice. However, some factors may lead to false‐positive diagnoses. This study aims to analyze the factors affecting clear imaging of the fallopian tubes in 4D‐HyCoSy and explore methods to improve the quality of fallopian tube imaging.MethodsA total of 118 patients were enrolled in this retrospective study. After injecting the SonoVue into the uterine cavity, three modes of HyCoSy were completed in sequence: 4D‐HyCoSy, 2D‐HyCoSy, and second harmonic imaging (SHI). Participants were divided into two groups: the easy visualization group (fallopian tubes could be visualized using only 4D‐HyCoSy) and the difficult visualization group (a multimodal combination was required for visualization). The position of the uterus, the relationship between the ovaries and the uterus, endometrial thickness, time of catheterization in the uterine cavity, presence or absence of lesions in the uterine cavity, whether intestinal gas covers the fallopian tubes and the imaging effect of different modes on the fallopian tubes was analyzed, to determine the key factors affecting the clear imaging of the fallopian tubes.ResultsThe positional relationship between the ovary and the uterus (OR = 4.711, 95% CI: 1.322–19.77, P = 0.023), the positioning of the uterus (OR = 3.843, 95% CI: 1.129–15.26, P = 0.04), endometrial thickness (OR = 3.985, 95% CI: 1.168–15.99, P = 0.036), and the duration of intrauterine catheter placement (OR = 3.547, 95% CI: 1.042–13.52, P = 0.05) were independent factors that affecting difficulty in visualizing the fallopian tubes.ConclusionUterine position, the positional relationship between the ovary and the uterus, endometrial thickness, and the time of catheter insertion are factors that affect visualizing the fallopian tubes during 4D‐HyCoSy. The combination of multimodal imaging, especially the combination of 4D‐HyCoSy with SHI mode, can help improve the quality of fallopian tube visualization.
Sjögren syndrome from a dermatological perspective: A retrospective study of 185 SSA‐Ro positive patients
Authors: André Fiona , Bujak Martha , Frischhut Nina , Kronbichler Andreas , Sepp Norbert , Ratzinger Gudrun , Böckle Barbara 
Published: July 2024

AbstractBackgroundSjögren syndrome (SS) is a systemic autoimmune disease characterised by oral and ocular mucosal dryness.ObjectivesOur study focused on skin manifestations of SS. The differences between primary SS (pSS) and secondary SS (sSS) were investigated. Furthermore, we elucidated factors predicting internal organ involvement.MethodsThis was a single centre retrospective cohort study. We analysed clinical and laboratory data from 185 Ro‐SSA positive SS patients between January 2000 and December 2016.ResultsSkin manifestations were present in 74.9% (n = 137) of SS patients. Dermatitis and Lupus erythematosus (LE)‐specific skin manifestations were present in 34.1% (n = 63) and 21.6% (n = 40) of SS patients respectively. 65.9% (n = 122) of SS patients had internal organ involvement, with 53% (n = 98) presenting neuropsychiatric manifestations. Several risk factors for internal organ involvement were identified: presence of myalgia/arthralgia (odds ratio [OR] = 3.05, confidence interval [CI] 1.29−7.22), fatigue (OR = 2.53, CI 1.11−5.73) and age older than 50 years (OR = 2.23, CI 1.01−4.93).ConclusionsInternal organ involvement is frequent in SS, however there is no significant difference between pSS and sSS concerning its occurrence.
Two pemphigoid cases with mucous membrane involvement successfully treated with baricitinib
Authors: Malkovics Tamás , Horváth Orsolya N. , Sárdy Miklós 
Published: July 2024

AbstractBoth bullous pemphigoid and mucous membrane pemphigoid are autoimmune bullous diseases that can be challenging to treat in individual cases. Janus kinase inhibitors have shown efficacy in several autoimmune and inflammatory diseases, although these drugs are not yet widely used in autoimmune bullous diseases. We report two recalcitrant cases of pemphigoid with mucous membrane involvement which were successfully treated with the JAK 1/2 inhibitor baricitinib after all other feasible therapeutic options had been exhausted.