bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiple Percutaneous Longitudinal Tenotomies for Chronic Achilles Tendinopathy in Runners
Authors: Maffulli Nicola , Oliva Francesco , Testa Vittorino , Capasso Giovanni , Del Buono Angelo 
Published: July 2013

Background: Multiple percutaneous longitudinal tenotomies have been successfully undertaken in runners with isolated midportion Achilles tendinopathy (nodular lesions <2.5 cm) without any sign of paratendinopathy. Hypothesis: In the long term, minimally invasive multiple tenotomies allow the patient to remain involved in middle- and long-distance running. Clinical and ultrasound (US) evidence of paratendinopathy is a negative prognostic factor. Study Design: Case series study; Level of evidence, 4. Methods: A total of 39 patients were reviewed at an average follow-up of 17 years (range, 15-22 years) after US-guided multiple percutaneous longitudinal tenotomies for chronic Achilles tendinopathy. The Victorian Institute of Sports Assessment–Achilles (VISA-A) questionnaire was completed by each patient, maximum calf circumference and isometric plantarflexion strength of the gastrocsoleus complex were measured in both the affected and contralateral legs, and functional assessment was scored by the 4-point Boyden scale. Achilles tendon changes were also assessed according to the grayscale US grading system. Results: At the final follow-up, the maximum calf circumference and the strength in the operated leg were not significantly different than those measured preoperatively, but they were significantly lower than those on the contralateral side. This did not affect patients’ daily or sports activities. All patients had returned to their preinjury working occupation; 20 patients were still active in middle- and long-distance running, with an average current level of sport and function that was 60% ± 13% compared with baseline status (before onset of symptoms). Thirty of 39 patients (77%) reported good or excellent outcomes according to the Boyden assessment. On US assessment, the tendon was generally thicker than the contralateral asymptomatic tendon (average, 7.0 vs 8.7 mm, respectively; P = .003). There was no significant difference ( P > .05) when comparing patients with good or excellent Boyden results versus those with fair or poor outcomes. The mean VISA-A score was 78.5, with no significant difference between patients with and without paratendinopathy on US assessment ( P > .05). All of the patients who did not return to running or who gave up sports activities had signs of paratendinopathy. Conclusion: This approach to the management of midportion Achilles tendinopathy is safe, has a low cost, and is effective in the long term.
Risk Factors for Lumbar Disc Degeneration in High School American Football Players
Authors: Nagashima Masaki , Abe Hitoshi , Amaya Kenji , Matsumoto Hideo , Yanaihara Hisashi , Nishiwaki Yuji , Toyama Yoshiaki , Matsumoto Morio 
Published: July 2013

Background: Several risk factors have been proposed for intervertebral disc degeneration (DD) among adolescent athletes. However, the causes of DD are not well understood, and there have been few prospective studies evaluating DD in adolescents. Purpose: To identify risk factors for DD among adolescent American football (AF) players. Study Design: Cohort study (prevalence); Level of evidence, 2. Methods: This study investigated the relationships between the progression of DD and the following factors: lumbar spine abnormalities on baseline radiographs, body mass index, AF position played (lineman or other), and length of playing career (2 full competitive AF seasons or <2 seasons). Included were 192 students who joined a top-ranked high school AF team from 2004 to 2008. Of these, 160 played for 2 full competitive AF seasons. The remaining 32 players, who stopped before completing 2 seasons, were used as a control group. Baseline radiographs and lumbar magnetic resonance imaging (MRI) scans were obtained when the players enrolled in the AF team in May, and follow-up lumbar MRI scans were obtained 2 years later at the end of their second academic year in March. Disc degeneration was measured by the signal intensity of the nucleus pulposus, and its progression was evaluated by multiple regression analysis of decreases in signal intensity. Also analyzed was the relationship between DD and low back pain (LBP). Results: The mean decrease in signal intensity of the nucleus pulposus was 4.30% ± 11.63% in players who completed 2 AF seasons and 1.41% ± 10.03% in those who did not ( P = .12). Mean visual analog scale scores for LBP at follow-up were significantly higher ( P = .001) in players who had played for 2 full seasons (2.67 ± 2.81) than in those with a shorter career (0.99 ± 1.61). Decreases in signal intensity of the nucleus pulposus after 2 years of playing AF related significantly to playing a lineman position (partial regression coefficient, 3.47%), the presence of Schmorl nodes (partial regression coefficient, 3.58%), and disc herniation (partial regression coefficient, 4.09%). Conclusion: Significant risk factors for DD progression in high school AF players included playing a lineman position, the presence of Schmorl nodes, and disc herniation. Continuing to play AF through 2 years of high school was a risk factor for the onset of LBP.
Léopold Sédar Senghor and the Civilization of the Universal
Authors: Mabana Kahiudi C. 
Published: July 2013

AbstractOne among the concepts strongly defended by Léopold Sédar Senghor is what he called the civilization of the universal, which forms an essential component of his thought. This concept has been thoroughly commented and, at times, wrongly or controversially interpreted. My approach consists of a personal reflection on the backgrounds of Senghor’s thought. First of all I will deal with Senghor’s conception of Negritude and what it implies in terms of black identification, recognition of Africa as birthplace of races. Then I will examine the civilization of the universal as defended by Senghor and its link to the thought of Father Teilhard de Chardin, a French palaeontologist. Finally I will present the passionate debate on his work and thought. Senghor was among the African writers and thinkers probably the most admired, the most awarded throughout the world, but also the most criticized and hated.
Transcending Cosmopolitanism
Authors: Ramose Mogobe Bernard 
Published: July 2013

AbstractIn general, discussion about cosmopolitanism is predicated on the assumption of community either already existing or yet to be realised. A common feature of this assumption is that community represents the boundary of “us” inclusion and exclusion of “the other”. Accordingly, the strife to reach out to “the other” constitutes the quest for the cosmos – order – and its realisation is cosmopolitanism. Cosmopolitanism is thus based on the ontology of opposition tending towards synthesis. The thesis defended here is that the ontology of boundary as a coupling point and not a moment of the exclusion of “the other” is a necessary reaffirmation as well as complement of “us”. This ontology, supported by polylogue among the peoples and cultures of our planet, is the means to transcend cosmopolitanism and constitute a planetary human community.
Obinutuzumab (GA101) plus CHOP or FC in relapsed/refractory follicular lymphoma: results of the GAUDI study (BO21000)
Authors: Radford John , Davies Andrew , Cartron Guillaume , Morschhauser Franck , Salles Gilles , Marcus Robert , Wenger Michael , Lei Guiyuan , Wassner-Fritsch Elisabeth , Vitolo Umberto 
Published: July 2013

Key Points
Obinutuzumab is a novel type II anti-CD20 monoclonal antibody under early-stage clinical investigation. Obinutuzumab plus CHOP or FC has an acceptable safety profile and is effective in relapsed/refractory follicular lymphoma.
HLA specificities are related to development and prognosis of diffuse large B-cell lymphoma
Authors: Alcoceba Miguel , Sebastián Elena , Marín Luis , Balanzategui Ana , Sarasquete M. Eugenia , Chillón M. Carmen , Jiménez Cristina , Puig Noemí , Corral Rocío , Pardal Emilia , Grande Carlos , Bello José Luis , Albo Carmen , de la Cruz Fátima , Panizo Carlos , Martín Alejandro , González-Barca Eva , Caballero M. Dolores , San Miguel Jesús F. , García-Sanz Ramón , González Marcos 
Published: July 2013

Key PointsDLBCL patients carrying the HLA-B44 supertype have a worse progression-free and overall survival after R-CHOP-like treatment. The HLA-DRB1*01 allele increases the risk of DLBCL development.
Development of gene therapy for blood disorders: an update
Authors: Nienhuis Arthur W. 
Published: July 2013

Abstract
This review addresses the current status of gene therapy for immunodeficiencies, chronic granulomatous disease, suicide gene therapy for graft-versus-host disease, viral infections, malignant hematologic disorders, hemophilia, and the hemoglobin disorders. New developments in vector design have fostered improved expression as well as enhanced safety, particularly of integrating retroviral vectors. Several immunodeficiencies have been treated successfully by stem cell–targeted, retroviral-mediated gene transfer with reconstitution of the immune system following infusion of the transduced cells. In a trial for hemophilia B, long-term expression of human FIX has been observed following adeno-associated viral vector–mediated gene transfer into the liver. This approach should be successful in treating any disorder in which liver production of a specific protein is therapeutic.
Genomics analysis of potassium channel genes in songbirds reveals molecular specializations of brain circuits for the maintenance and production of learned vocalizations
Authors: Lovell Peter V , Carleton Julia B , Mello Claudio V 
Published: July 2013

Abstract

Background
A fundamental question in molecular neurobiology is how genes that determine basic neuronal properties shape the functional organization of brain circuits underlying complex learned behaviors. Given the growing availability of complete vertebrate genomes, comparative genomics represents a promising approach to address this question. Here we used genomics and molecular approaches to study how ion channel genes influence the properties of the brain circuitry that regulates birdsong, a learned vocal behavior with important similarities to human speech acquisition. We focused on potassium (K-)Channels, which are major determinants of neuronal cell excitability.
Starting with the human gene set of K-Channels, we used cross-species mRNA/protein alignments, and syntenic analysis to define the full complement of orthologs, paralogs, allelic variants, as well as novel loci not previously predicted in the genome of zebra finch (Taeniopygia guttata). We also compared protein coding domains in chicken and zebra finch orthologs to identify genes under positive selective pressure, and those that contained lineage-specific insertions/deletions in functional domains. Finally, we conducted comprehensive in situ hybridizations to determine the extent of brain expression, and identify K-Channel gene enrichments in nuclei of the avian song system.

Results
We identified 107 K-Channel finch genes, including 6 novel genes common to non-mammalian vertebrate lineages. Twenty human genes are absent in songbirds, birds, or sauropsids, or unique to mammals, suggesting K-Channel properties may be lineage-specific. We also identified specific family members with insertions/deletions and/or high dN/dS ratios compared to chicken, a non-vocal learner. In situ hybridization revealed that while most K-Channel genes are broadly expressed in the brain, a subset is selectively expressed in song nuclei, representing molecular specializations of the vocal circuitry.

Conclusions
Together, these findings shed new light on genes that may regulate biophysical and excitable properties of the song circuitry, identify potential targets for the manipulation of the song system, and reveal genomic specializations that may relate to the emergence of vocal learning and associated brain areas in birds.
Abnormal activity of default mode network in GERD patients
Authors: Sun Huihui , Chen Ying , Zhao Xiaohu , Wang Xiangbin , Jiang Yuanxi , Wu Ping , Tang Yinhan , Meng Qingwei , Xu Shuchang 
Published: July 2013

AbstractBackgroundAbnormal processing of esophageal sensation at the level of the central nervous system has been proven to be involved in gastroesophageal reflux disease (GERD). However, most studies were focused on the possible functions of perceptual processing related network during task status, little attention has been paid to default mode network, which has been manifested to be important in the pathogenesis of many diseases. In our study, we compared the brain activity characteristic in GERD patients with the healthy subjects (HS) at baseline, looking for whether activities of default mode network were abnormal in GERD patients and attempting to identify their possible roles in GERD. In present study, fractional amplitude of low-frequency fluctuation was adopted to detect the brain activities at baseline. Group-level analyses were conducted by one-sample t test within groups (voxel thresholds were p < 0.001 and cluster level >42, corrected P < 0.05) and independent-samples t test between groups (p < 0.01 and cluster level >90, corrected P < 0.05) using SPM5.ResultsThe predominant activity area in both groups mainly located in default mode network such as medial superior frontal gyrus, precuneus, posterior cingulate gyrus, etc. However, the activities of precuneus and posterior cingulate gyrus were significantly lower in GERD patients than those in the HS.ConclusionsThe activities of precuneus and posterior cingulate gyrus of default mode network in GERD patients were significantly lower compared to the HS, suggesting abnormal activities of brain regions in default mode network may be involved in pathophysiology of GERD symptom generation.
The dual targeting ability of type II NAD(P)H dehydrogenases arose early in land plant evolution
Authors: Xu Lin , Law Simon R , Murcha Monika W , Whelan James , Carrie Chris 
Published: July 2013

Abstract

Background
Type II NAD(PH) dehydrogenases are located on the inner mitochondrial membrane of plants, fungi, protists and some primitive animals. However, recent observations have been made which identify several Arabidopsis type II dehydrogenases as dual targeted proteins. Targeting either mitochondria and peroxisomes or mitochondria and chloroplasts.

Results
Members of the ND protein family were identified in various plant species. Phylogenetic analyses and subcellular targeting predictions were carried out for all proteins. All ND proteins from three model plant species Arabidopsis, rice and Physcomitrella were cloned as N- and C-terminal GFP fusions and subcellular localisations were determined. Dual targeting of plant type II dehydrogenases was observed to have evolved early in plant evolution and to be widespread throughout different plant species. In all three species tested dual targeting to both mitochondria and peroxisomes was found for at least one NDA and NDB type protein. In addition two NDB type proteins from Physcomitrella were also found to target chloroplasts. The dual targeting of NDC type proteins was found to have evolved later in plant evolution.

Conclusions
The functions of type II dehydrogenases within plant cells will have to be re-evaluated in light of this newly identified subcellular targeting information.
Risk factors of recurrence and life-threatening complications for patients hospitalized with chronic suppurative osteomyelitis of the jaw
Authors: Chen Lin , Li Tangxin , Jing Wei , Tang Wei , Tian Weidong , Li Cai , Liu Lei 
Published: July 2013

Abstract

Background
Clinically, recurrence and life-threatening complications are challenging problems for chronic suppurative osteomyelitis of the jaw (CSOJ), but there is no quantitative analysis or report about the causes of or risk factors for the two problems to date. Doctors identify the causes or risk factors only through clinical experience. We performed a retrospective study of 322 patients with CSOJ to quantificationally analysed the risk factors for the abovementioned two problems by logistic regression analysis.

Methods
A retrospective study of 322 patients hospitalized with CSOJ was performed. The socio-demographic and clinical characteristics were recorded. The risk factors for the above two problems were analyzed by logistic regression analysis. Frequency and percentage were used to indicate descriptive research factors. A univariate logistic regression analysis was performed to calculate the odds ratio (OR) and to identify independent risk factors. The independent risk factors were further identified by multivariate logistic regression analysis.

Results
An age from 6–12 years or > 65 years, pre-admission antibiotic administration, a lesion at the mandibular ramus, concurrent maxillofacial space infection (MSI), and conservation of pathogenic teeth were found to be risk factors for recurrence. An age > 65 years, admission temperature > 39 degree Celsius, admission white blood cell (WBC) count >15×109/L, pre-admission antibiotic administration, concurrent MSI, pre-existing diabetes, and respiratory difficulty were found to be risk factors for life-threatening complications.

Conclusions
The results indicate that doctors should remain mindful of the risk factors listed above, and the management of CSOJ should be increasingly aggressive when the above risk factors are present, especially when the lesion is located at the mandibular ramus. In addition, pathogenic teeth must be extracted, and antibiotics should be administered properly.

Trial registration
Clinicaltrials.gov (NCT01670422)
Crosslinking and mass spectrometry suggest that the isolated NTD domain dimer of Moloney murine leukemia virus integrase adopts a parallel arrangement in solution
Authors: Henriquez Daniel R , Zhao Caifeng , Zheng Haiyan , Arbildua José J , Acevedo Mónica L , Roth Monica J , Leon Oscar 
Published: July 2013

Abstract

Background
Retroviral integrases (INs) catalyze the integration of viral DNA in the chromosomal DNA of the infected cell. This reaction requires the multimerization of IN to coordinate a nucleophilic attack of the 3’ ends of viral DNA at two staggered phosphodiester bonds on the recipient DNA. Several models indicate that a tetramer of IN would be required for two-end concerted integration. Complementation assays have shown that the N-terminal domain (NTD) of integrase is essential for concerted integration, contributing to the formation of a multimer through protein-protein interaction. The isolated NTD of Mo-MLV integrase behave as a dimer in solution however the structure of the dimer in solution is not known.

Results
In this work, crosslinking and mass spectrometry were used to identify regions involved in the dimerization of the isolated Mo-MLV NTD. The distances between the crosslinked lysines within the monomer are in agreement with the structure of the NTD monomer found in 3NNQ. The intermolecular crosslinked peptides corresponding to Lys 20-Lys 31, Lys 24-Lys 24 and Lys 68-Lys 88 were identified. The 3D coordinates of 3NNQ were used to derive a theoretical structure of the NTD dimer with the suite 3D-Dock, based on shape and electrostatics complementarity, and filtered with the distance restraints determined in the crosslinking experiments.

Conclusions
The crosslinking results are consistent with the monomeric structure of NTD in 3NNQ, but for the dimer, in our model both polypeptides are oriented in parallel with each other and the contacting areas between the monomers would involve the interactions between helices 1 and helices 3 and 4.
Development and evaluation of a de-identification procedure for a case register sourced from mental health electronic records
Authors: Fernandes Andrea C , Cloete Danielle , Broadbent Matthew TM , Hayes Richard D , Chang Chin-Kuo , Jackson Richard G , Roberts Angus , Tsang Jason , Soncul Murat , Liebscher Jennifer , Stewart Robert , Callard Felicity 
Published: July 2013

Abstract

Background
Electronic health records (EHRs) provide enormous potential for health research but also present data governance challenges. Ensuring de-identification is a pre-requisite for use of EHR data without prior consent. The South London and Maudsley NHS Trust (SLaM), one of the largest secondary mental healthcare providers in Europe, has developed, from its EHRs, a de-identified psychiatric case register, the Clinical Record Interactive Search (CRIS), for secondary research.

Methods
We describe development, implementation and evaluation of a bespoke de-identification algorithm used to create the register. It is designed to create dictionaries using patient identifiers (PIs) entered into dedicated source fields and then identify, match and mask them (with ZZZZZ) when they appear in medical texts. We deemed this approach would be effective, given high coverage of PI in the dedicated fields and the effectiveness of the masking combined with elements of a security model. We conducted two separate performance tests i) to test performance of the algorithm in masking individual true PIs entered in dedicated fields and then found in text (using 500 patient notes) and ii) to compare the performance of the CRIS pattern matching algorithm with a machine learning algorithm, called the MITRE Identification Scrubber Toolkit – MIST (using 70 patient notes – 50 notes to train, 20 notes to test on). We also report any incidences of potential breaches, defined by occurrences of 3 or more true or apparent PIs in the same patient’s notes (and in an additional set of longitudinal notes for 50 patients); and we consider the possibility of inferring information despite de-identification.

Results
True PIs were masked with 98.8% precision and 97.6% recall. As anticipated, potential PIs did appear, owing to misspellings entered within the EHRs. We found one potential breach. In a separate performance test, with a different set of notes, CRIS yielded 100% precision and 88.5% recall, while MIST yielded a 95.1% and 78.1%, respectively. We discuss how we overcome the realistic possibility – albeit of low probability – of potential breaches through implementation of the security model.

Conclusion
CRIS is a de-identified psychiatric database sourced from EHRs, which protects patient anonymity and maximises data available for research. CRIS demonstrates the advantage of combining an effective de-identification algorithm with a carefully designed security model. The paper advances much needed discussion of EHR de-identification – particularly in relation to criteria to assess de-identification, and considering the contexts of de-identified research databases when assessing the risk of breaches of confidential patient information.