bluuebunny/crossref_metadata_2025_split huggingface dataset:
A provincial adaptation of clinical practice guidelines for depression in primary care: a case illustration of the ADAPTE method
Authors: Roberge Pasquale , Fournier Louise , Brouillet Hélène , Delorme André , Beaucage Clément , Côté Rodrigue , Demers Pierre , Gervais Michel , Laflamme France , Latulippe Louise , Marchand André , Patry Simon , Pelchat Suzanne , Provencher Martin D. , Provost Jean‐Rémy , Robitaille David , Cloutier Anne‐Marie 
Published: June 2015

AbstractRationale, aims and objectivesMental health services for patients with a major depressive disorder are commonly delivered by primary care. To support the uptake of clinical practice guidelines in primary care, we developed and disseminated a practice protocol for depression tailored for a multidisciplinary audience of primary mental health care providers with the ADAPTE methodology. The research questions addressed in this study aimed at examining the experience of the development process of a mental health practice protocol in terms of adaptation, facilitation and implementation.MethodsWe present a descriptive case study of the development and implementation of a practice protocol for major depressive disorder for primary mental health care in the organizational and cultural context of the province of Québec (Canada), following the steps of the ADAPTE methodology. An expert committee composed of general practitioners, mental health specialists, health care administrators and decision makers at regional and provincial levels participated in the protocol development process.ResultsThe practice protocol was based on two clinical practice guidelines: the NICE guideline on the treatment and management of depression in adults (2009, 2010) and the Canadian Network for Mood and Anxiety Treatments clinical guidelines for the management of major depressive disorder in adults (2009). A stepped care model was embedded in the protocol to facilitate the implementation of clinical recommendations in primary mental health care. A multifaceted dissemination strategy was used to support the uptake of the protocol recommendations in clinical practice.ConclusionsThe ADAPTE methodology provided structure, rigour and efficiency to the trans‐contextual adaptation of guideline recommendations. We will share the challenges associated with the adaptation of clinical recommendations and organizational strategies for a mental health guideline, and the dissemination of the practice protocol in primary care.
Short‐term modern life‐like stress exacerbates Aβ‐pathology and synapse loss in 3xTg‐AD mice
Authors: Baglietto‐Vargas David , Chen Yuncai , Suh Dongjin , Ager Rahasson R. , Rodriguez‐Ortiz Carlos J. , Medeiros Rodrigo , Myczek Kristoffer , Green Kim N. , Baram Tallie Z. , LaFerla Frank M. 
Published: June 2015

AbstractAlzheimer's disease (AD) is a progressive neurological disorder that impairs memory and other cognitive functions in the elderly. The social and financial impacts of AD are overwhelming and are escalating exponentially as a result of population aging. Therefore, identifying AD‐related risk factors and the development of more efficacious therapeutic approaches are critical to cure this neurological disorder. Current epidemiological evidence indicates that life experiences, including chronic stress, are a risk for AD. However, it is unknown if short‐term stress, lasting for hours, influences the onset or progression of AD. Here, we determined the effect of short‐term, multi‐modal ‘modern life‐like’ stress on AD pathogenesis and synaptic plasticity in mice bearing three AD mutations (the 3xTg‐AD mouse model). We found that combined emotional and physical stress lasting 5 h severely impaired memory in wild‐type mice and tended to impact it in already low‐performing 3xTg‐AD mice. This stress reduced the number of synapse‐bearing dendritic spines in 3xTg‐AD mice and increased Aβ levels by augmenting AβPP processing. Thus, short‐term stress simulating modern‐life conditions may exacerbate cognitive deficits in preclinical AD by accelerating amyloid pathology and reducing synapse numbers.
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Epidemiological evidence indicates that life experiences, including chronic stress, are a risk for Alzheimer disease (AD). However, it is unknown if short stress in the range of hours influences the onset or progression of AD. Here, we determined the effect of short, multi‐modal ‘modern‐lifelike'stress on AD pathogenesis and synaptic plasticity in mice bearing three AD mutations (the 3xTg‐AD mouse model). We found that combined emotional and physical stress lasting 5 h severely impaired memory in wild‐type mice and tended to impact it in already low‐performing 3xTg‐AD mice. This stress reduced the number of synapse‐bearing dendritic spines in 3xTg‐AD mice and increased Aβ levels by augmenting AβPP processing. Thus, short stress simulating modern‐life conditions may exacerbate cognitive deficits in preclinical AD by accelerating amyloid pathology and reducing synapse numbers.
Neuroprotective arylpiperazine dopaminergic/serotonergic ligands suppress experimental autoimmune encephalomyelitis in rats
Authors: Popovic Marjan , Stanojevic Zeljka , Tosic Jelena , Isakovic Aleksandra , Paunovic Verica , Petricevic Sasa , Martinovic Tamara , Ciric Darko , Kravic‐Stevovic Tamara , Soskic Vukic , Kostic‐Rajacic Sladjana , Shakib Kaveh , Bumbasirevic Vladimir , Trajkovic Vladimir 
Published: June 2015

AbstractArylpiperazine‐based dopaminergic/serotonergic ligands exert neuroprotective activity. We examined the effect of arylpiperazine D2/5‐HT1A ligands, N‐{4‐[2‐(4‐phenyl‐piperazin‐1‐yl)‐ethyl}‐phenyl]‐picolinamide (6a) and N‐{3‐[2‐(4‐phenyl‐piperazin‐1‐yl)‐ethyl]‐phenyl}‐picolinamide (6b), in experimental autoimmune encephalomyelitis (EAE), a model of neuroinflammation. Both compounds (10 mg/kg i.p.) reduced EAE clinical signs in spinal cord homogenate‐immunized Dark Agouti rats. Compound 6b was more efficient in delaying the disease onset and reducing the maximal clinical score, which correlated with its higher affinity for D2 and 5‐HT1A receptors. The protection was retained if treatment was limited to the effector (from day 8 onwards), but not the induction phase (day 0–7) of EAE. Compound 6b reduced CNS immune infiltration and expression of mRNA encoding the proinflammatory cytokines tumor necrosis factor, IL‐6, IL‐1, and GM‐CSF, TH1 cytokine IFN‐γ, TH17 cytokine IL‐17, as well as the signature transcription factors of TH1 (T‐bet) and TH17 (RORγt) cells. Arylpiperazine treatment reduced apoptosis and increased the activation of anti‐apoptotic mediators Akt and p70S6 kinase in the CNS of EAE animals. The in vitro treatment with 6b protected oligodendrocyte cell line OLN‐93 and neuronal cell line PC12 from mitogen‐activated normal T cells or myelin basic protein‐activated encephalitogenic T cells. In conclusion, arylpiperazine dopaminergic/serotonergic ligands suppress EAE through a direct neuroprotective action and decrease in CNS inflammation.
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Arylpiperazine dopaminergic/serotonergic ligands reduce neurological symptoms of acute autoimmune encephalomyelitis in rats without affecting the activation of autoreactive immune response, through mechanisms involving a decrease in CNS immune infiltration, as well as direct protection of CNS from immune‐mediated damage. These data indicate potential usefulness of arylpiperazine‐based compounds in the treatment of neuroinflammatory disorders such as multiple sclerosis.
Percutaneous Closure of Atrial Septal Defects Under Transthoracic Echocardiography Guidance Without Fluoroscopy or Intubation in Children
Authors: Pan Xiang‐Bin , Ou‐yang Wen‐Bin , Pang Kun‐Jing , Zhang Feng‐Wen , Wang Shou‐Zheng , Liu Yao , Zhang Da‐Wei , Guo Gai‐Li , Tian Peng‐Sheng , Hu Sheng‐Shou 
Published: June 2015

ObjectiveDemonstrate the benefits of percutaneous atrial septal defect (ASD) closure under guidance of transthoracic echocardiography (TTE) without fluoroscopy.MethodsFrom February 2013 to April 2014, 127 consecutive patients with an isolated type II ASD were recruited to undergo percutaneous closure under either TTE (n = 60, TTE group) or TEE (n = 67, TEE group) guidance. The TTE group received local anesthesia or sedation with propofol, and the TEE group received general anesthesia with endotracheal intubation. Follow‐up examinations were performed for both groups at 1 month, 3 months, 6 months, and 1 year after discharge and annually thereafter.ResultsThe TTE group had a significantly shorter procedure time and respirator ventilation duration than the TEE group. The dose of propofol required, the cost, and the pharyngeal complication rate were significantly lower in the TTE group than in the TEE group. The median follow‐up of 11.6 months was uneventful in all patients.ConclusionsPercutaneous ASD closure with TTE guidance as the only imaging tool avoids fluoroscopy, endotracheal intubation, and probe insertion and is associated with a satisfactory procedural success rate and lower costs. This procedure is a safe and reliable treatment for ASD.
Biological and Molecular Characterization of Alfalfa Mosaic Virus Infecting Trumpet Creeper (Campsis radicans) in Iran
Authors: Pourrahim Reza , Farzadfar Shirin 
Published: June 2015

AbstractSamples of trumpet creeper (Campsis radicans) leaves showing mottling and mosaic were collected from plants growing in a private garden in Tehran province, Iran, in 2012. Symptomatic leaf samples were tested for Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV) and Peanut stunt virus (PSV) infection in enzyme‐linked immunosorbent assay (ELISA), using specific antibodies. None of the samples were positive for CMV and PSV; however, all reacted positively with that of AMV antiserum. In biological assay, systemic infection was found on Datura stramonium, Nicotiana tabacum cvs., White Burley, and Xanthi, 21 days postinoculation (DPI), while necrotic local lesions were obtained following inoculation of Phaseolus vulgaris and Vigna unguiculata within three to four DPI. Using a pair of primers specific for AMV, a DNA fragment of 880 bp was RT‐PCR‐amplified. Analysis of the sequences revealed the presence of 657 nucleotides of AMV complete coat protein (CP) gene (translating 218 amino acid residues). Phylogenetic analysis using neighbour‐joining (NJ) method clustered AMV isolates into two main types and the IRN‐Tru (GenBank Accession No. JX865593) isolate fell into type I. Pairwise nucleotide distances also confirmed two main types with the highest and lowest similarities for type I and II, respectively. The association of AMV with mosaic disease of C. radicans represents the first record from the world.
Effects of dendrimer/cyclodextrin conjugates as gene transfer carriers on nitric oxide production from macrophages
Authors: Motoyama Keiichi , Sako Asumi , Ibrahim Abu Hashim Irhan , Higashi Taishi , Arima Hidetoshi 
Published: June 2015

Abstract

Objectives
The development of safe gene transfer carriers with high transfection efficiency, which does not affect the cell function, is a challenging issue. In this study, we examined the effects of α-cyclodextrin (α-CyD)/dendrimer conjugate (α-CDE (G3)) on nitric oxide (NO) production in murine macrophages J774.1 cells stimulated with toll-like receptors (TLR) ligands.

Methods
NO production from macrophages stimulated with TLR ligands was determined by the Griess method. Transfection efficiency of α-CDE (G3)/plasmid DNA (pDNA) complex was quantified by a luminometer.

Key findings
α-CDE (G3) significantly inhibited NO production from J774.1 cells stimulated with TLR ligands. α-CyD molecules in α-CDE (G3) had no effect on NO production. The inhibitory effect of α-CDE (G3) on NO production might be attributed to the dendrimer (G3). Increasing the degree of substitution (DS) of α-CyD in the α-CDE (G3) molecule was accompanied by a significant decrease in the inhibition of NO production. Furthermore, higher gene transfection efficiency of α-CDE (G3)/pDNA complex was observed upon increasing the DS of α-CyD.

Conclusions
α-CDE (G3) with high DS value of α-CyD may be considered as a safe gene transfer carrier that does not adversely affect NO production from macrophages stimulated with TLR ligands.
Analysis of rbcL sequences reveals the global biodiversity, community structure, and biogeographical pattern of thermoacidophilic red algae (Cyanidiales)
Authors: Hsieh Chia‐Jung , Zhan Shing Hei , Lin Yiching , Tang Sen‐Lin , Liu Shao‐Lun 
Published: June 2015

Thermoacidophilic cyanidia (Cyanidiales) are the primary photosynthetic eukaryotes in volcanic areas. These red algae also serve as important model organisms for studying life in extreme habitats. The global biodiversity and community structure of Cyanidiales remain unclear despite previous sampling efforts. Here, we surveyed the Cyanidiales biodiversity in the Tatun Volcano Group (TVG) area in Taiwan using environmental DNA sequencing. We generated 174 rbcL sequences from eight samples from four regions in the TVG area, and combined them with 239 publicly available rbcL sequences collected worldwide. Species delimita‐tion using this large rbcL data set suggested at least 20 Cyanidiales OTUs (operational taxono‐mic units) worldwide, almost three times the presently recognized seven species. Results from environmental DNA showed that OTUs in the TVG area were divided into three groups: (i) dominant in hot springs with 92%–99% sequence identity to Galdieria maxima; (ii) largely distributed in drier and more acidic microhabitats with 99% identity to G. partita; and (iii) primarily distributed in cooler microhabitats and lacking identity to known cyanidia species (a novel Cyanidiales lineage). In both global and individual area analyses, we observed greater species diversity in non‐aquatic than aquatic habitats. Community structure analysis showed high similarity between the TVG community and West Pacific‐Iceland communities, reflecting their geographic proximity to each other. Our study is the first examination of the global species diversity and biogeographic affinity of cyanidia. Additionally, our data illuminate the influence of microhabitat type on Cyanidiales diversity and highlight intriguing questions for future ecological research.
Generic identity of Camptorrhiza indica (Colchicaceae) based on cytogenetics and molecular phylogenetics
Authors: Lekhak Manoj M. , Surveswaran Siddharthan , Yadav Shrirang R. 
Published: June 2015

AbstractThe tribe Iphigenieae (Colchicaceace, Liliales) includes two genera, viz. Camptorrhiza and Iphigenia, which are distributed in Africa, India, and Australasia. Iphigenia is represented by 12 species, of which six occur in India while Camptorrhiza comprises one species each in Africa (C. strumosa) and India (C. indica). The genus Camptorrhiza possesses a knee‐shaped tuber attached to the corms, filaments with a thick bulge in the middle and styles with single stigma. Iphigenia on the other hand lacks knee‐shaped tuber, bears linear filaments and has styles with three stigmas. Camptorrhiza indica possesses ovoid corms, linear filaments and styles with a single stigma. These characters are intermediate between Iphigenia and Camptorrhiza and hence we studied the cytogenetics and phylogenetic placement of this species to ascertain its generic identity. Somatic chromosome count (2n = 22) and karyotypic features of C. indica are very similar to that of Iphigenia species. Molecular phylogenetic studies based on atpB‐rbcL, rps16, trnL, and trnL‐F regions showed that C. indica is nested within a lineage of Indian Iphigenia species. Thus, C. indica was reduced to a species of Iphigenia, i.e., I. ratnagirica. Camptorrhiza is now a monotypic genus restricted only to southern Africa. A key to the Indian Iphigenia species is provided. In addition, a new combination Wurmbea novae‐zelandiae is proposed for Iphigenia novae‐zelandiae.
Effect of Defatted Wheat Germ Content and Its Particle Size on the Rheological and Textural Properties of the Cookie Dough
Authors: Petrović Jovana , Fišteš Aleksandar , Rakić Dušan , Pajin Biljana , Lončarević Ivana , Šubarić Drago 
Published: June 2015

AbstractThe Box–Behnken experimental design and modern instrumental methods were applied to examine the rheological and textural characteristics of the cookie dough, depending on the amount of flour replaced with wheat germ (5, 10 and 15%), different particle sizes of wheat germ (<150, 150–1,000, 800–2,000 μm) and varying moisture content of the dough (20, 22 and 24%). The results showed that increasing particle size of the wheat germ reduces deformation compliance of the dough. Moisture content had the greatest impact on dough elastic and viscous modulus. Particle size of wheat germ had an impact on resistance to extension and on extensibility of the dough. Wheat germ content up to 15% had no influence on the rheological and textural characteristics of the cookie dough.Practical ApplicationsWheat germ is nutritionally the most valuable part of the grain of wheat. It is a rich source of vitamin E, B vitamins, protein, dietary fiber and minerals (calcium, iron, potassium, magnesium, zinc). Unfortunately, wheat germ is one of the main by‐products of milling because of its high oil content. Using defatted wheat germ (by‐product of the wheat germ oil removing process), we can nutritionally enrich cookies that are ready‐to‐eat, have long shelf life and widely consumed food. The present results indicate that the combination of wheat germ content, its particle size and moisture of the dough can be used to improve the nutritional quality of the cookies, without a negative effect on the physical characteristics of the dough.
Symmetric Dimethylarginine Assay Validation, Stability, and Evaluation as a Marker for the Early Detection of Chronic Kidney Disease in Dogs
Authors: Nabity M.B. , Lees G.E. , Boggess M.M. , Yerramilli M. , Obare E. , Yerramilli M. , Rakitin A. , Aguiar J. , Relford R. 
Published: June 2015

BackgroundSymmetric dimethylarginine (SDMA) is a small molecule formed by methylation of arginine, and released into blood during protein degradation.SDMAis primarily eliminated by renal excretion and is a promising endogenous marker of glomerular filtration rate (GFR).ObjectivesTo validate an assay forSDMAmeasurement, determine stability ofSDMAin blood, and compareSDMAwith serum creatinine concentration (sCr) andGFRfor early detection of decreasing kidney function in dogs with chronic kidney disease (CKD).AnimalsEight male dogs affected with X‐linked hereditary nephropathy and 4 unaffected male littermates.MethodsProspective study validatingSDMAmeasurement using liquid chromatography‐mass spectrometry, assessing stability ofSDMAin serum and plasma, and serially determiningsCr,SDMA, andGFR(using iohexol clearance) in dogs during progression from preclinical disease to end‐stage renal failure. Correlations were determined using linear regression. Timepoints at whichsCr,SDMA, andGFRidentified decreased renal function were compared using defined cutoffs, trending in an individual dog, and comparison with unaffected littermates.ResultsSymmetric dimethylarginine was highly stable in serum and plasma, and the assay demonstrated excellent analytical performance. In unaffected dogs,SDMAremained unchanged whereas in affected dogs,SDMAincreased during disease progression, correlating strongly with an increase insCr (r = 0.95) and decrease inGFR(r = −0.95). Although trending improvedsCr's sensitivity,SDMAidentified, on average, <20% decrease inGFR, which was earlier thansCr using any comparison method.Conclusions and Clinical ImportanceSymmetric dimethylarginine is useful for both early identification and monitoring of decreased renal function in dogs withCKD.
Risk factors for primary sclerosing cholangitis
Authors: Boonstra Kirsten , de Vries Elisabeth M. G. , van Geloven Nan , van Erpecum Karel J. , Spanier Marcel , Poen Alexander C. , van Nieuwkerk Carin M. , Witteman Ben J. , Tuynman Hans A. , Naber Anton H. , Kingma Paul J. , Beuers Ulrich , Ponsioen Cyriel Y. ,   the Epi PSC PBC Study Group
Published: June 2015

AbstractBackground & AimsPrimary sclerosing cholangitis (PSC) is a progressive cholestatic liver disease of unknown cause, but strongly associated with inflammatory bowel disease (IBD). Potential risk factors triggering PSC have never been studied on a population level. The aim of this study was to evaluate smoking, appendectomy, family history and geographical distribution in a population‐based cohort of PSC patients, as compared to IBD control patients and healthy controls (HC).MethodsFor this case–control study 343 PSC patients, 370 IBD controls and 232 HC's living in a geographically defined area in the Netherlands filled‐out a questionnaire concerning smoking, appendectomy and family history of IBD and autoimmune liver diseases.ResultsSmoking was associated with a lower risk of developing PSC in PSC‐ulcerative colitis (UC) patients (adjusted OR 0.21; 95% CI 0.12–0.34; P < 0.001). Comparable results were found for PSC‐Crohn's disease (CD) patients (16% former smokers) compared to CD patients (55% former smokers) (adjusted OR 0.17; 95% CI 0.08–0.39; P < 0.001). Frequency of appendectomy did not differ between PSC and HC, but PSC‐UC patients had undergone appendectomy more often than UC patients (13% vs. 6%) (adjusted OR 2.51; 95%CI 1.04–6.07; P = 0.041). We found no association between family history of IBD or autoimmune liver disease and risk of PSC. Degree of urbanization was not associated with PSC incidence.ConclusionIn this large population‐based case–control study we confirm that smoking is associated with a lower risk of developing PSC, independent of its protective effect for developing UC. Appendectomy is not associated with the risk of developing PSC.
Testing the role of ecology and life history in structuring genetic variation across a landscape: a trait‐based phylogeographic approach
Authors: Paz Andrea , Ibáñez Roberto , Lips Karen R. , Crawford Andrew J. 
Published: June 2015

AbstractHypotheses to explain phylogeographic structure traditionally invoke geographic features, but often fail to provide a general explanation for spatial patterns of genetic variation. Organisms' intrinsic characteristics might play more important roles than landscape features in determining phylogeographic structure. We developed a novel comparative approach to explore the role of ecological and life‐history variables in determining spatial genetic variation and tested it on frog communities in Panama. We quantified spatial genetic variation within 31 anuran species based on mitochondrial DNA sequences, for which hierarchical approximate Bayesian computation analyses rejected simultaneous divergence over a common landscape. Regressing ecological variables, on genetic divergence allowed us to test the importance of individual variables revealing that body size, current landscape resistance, geographic range, biogeographic origin and reproductive mode were significant predictors of spatial genetic variation. Our results support the idea that phylogeographic structure represents the outcome of an interaction between organisms and their environment, and suggest a conceptual integration we refer to as trait‐based phylogeography.