bluuebunny/crossref_metadata_2025_split huggingface dataset:
CYHV-3 Infection Dynamics in Common Carp (Cyprinus Carpio) – Evaluation of Diagnostic Methods
Authors: Matras Marek , Antychowicz Jerzy , Castric Jeannette , Bergmann Sven M. 
Published: February 2013

Abstract
The KHV free carp were experimentally infected with CyHV-3 suspension by immersion at temperatures of 18ºC±1 and 25ºC±1. Skin, brain, gills, liver, spleen, kidneys, and leukocytes were investigated for the presence of KHV DNA, and sera for the presence of antibodies against the virus. Similar investigations were performed with non-infected carp, designed as control groups. The results of KHV DNA detection in the infected carp kept at 25ºC ±1 were compared with the results of the virus isolation onto common carp brain cells using leukocyte co-cultivation method and results of specific antibody detection. The appearance of specific antibodies at various time intervals after detecting the clinical symptoms of KHVD in naturally infected carp was additionally studied. At 18°C water temperature, KHV DNA was not detected up to the 6th d post infection (dpi). Then it was found in samples from skin, gill, liver, spleen, and kidneys and much later from the brain. At 25°C water temperature, KHV DNA was already detected on the 3rd dpi in samples from skin and gills, and from the 5th dpi it was found in all examined internal organs, except the brain where it was found at 6th dpi. At 25°C, isolations of CyHV-3 have succeeded between the 7th and 11th dpi. The first specific antibodies were found no sooner than on the 21st dpi. The serological examination performed in naturally infected carp in 15 carp pond farms showed that first specific antiviral antibodies can be detected 2 weeks after the appearance of clinical symptoms of KHVD.
Changes in Antibiotic Susceptibility Pattern of Atypical Staphylococcus Aureus Strains Isolated from Cows of the same Herd in 2008-2010
Authors: Małkińska-Horodyska Magdalena , Kubiak Joanna , Lassa Henryka , Malinowski Edward 
Published: February 2013

Abstract
The isolates of Staphylococcus aureus strains were examined phenotypically by cultural features, tube coagulase test and clumping factor (CF), and genotypically by conventional PCR. The strains had positive reaction in CF test, but were negative in tube coagulase test. The analysed strains from the same cows in each year expressed also nuc and coa genes. About 25% of the strains were examined by the disc diffusion method for their sensitivity to antibiotics. During three years, the strains were highly susceptible in vitro to amoxicillin with clavulanic acid, oxacillin, bacitracin, and cefoperazone (more than 90%), and highly resistant to tetracycline, neomycin, and streptomycin. Forty randomly chosen strains, and eight strains from the same cows in each year were analysed for minimal inhibitory concentration of penicillin G using microdilution method. An increasing resistance to the penicillin was noted. Moreover, eight strains, the same in each year, were also examined for β-lactamase production and methicillin resistance. No β-lactamase producers and no methicillin resistant strains were found using phenotypic and genotypic methods. In conclusion, it can be stated that antimicrobial susceptibility can change from one year to another.
Changes in Cocaine- and Amphetamine-Regulated Transcript-Like Immunoreactive (CART-LI) Nerve Structures of the Porcine Descending Colon During Proliferative Enteropathy
Authors: Gonkowski Sławomir , Burliński Piotr , Szwajca Piotr , Całka Jarosław 
Published: February 2013

Abstract
The distribution pattern of CART- like immunoreactive (CART-LI) nerve structures was studied by a double immunofluorescence technique in the circular muscle layer, myenteric (MP), outer submucous (OSP), and inner submucous plexuses (ISP), as well as in the mucosal layer of porcine descending colon under physiological conditions and during proliferative enteropathy (PE). In control animals, CART-LI perikarya have been shown to constitute 3.18 ±0.51%, 3.44 ±0.6%, and 3.33 ±0.72% in MP, OSP, and ISP, respectively. PE caused a decrease in the number of CART - LI neurons in MP and ISP. In OSP, the observed changes were not statistically significant. During PE, the number of CART-LI perikarya amounted to 2.18 ±0.27%, 3.07 ±0.06%, and 0.07 ±0.02% within MP, OSP, and ISP, respectively. Moreover, PE caused an increase in the number of CART-LI nerve fibers in the colonic circular muscle and mucosal layers as well as in MP. This study describes for the first time changes in CART-LI nerve structures of the porcine descending colon during Lawsonia intracellularis infection, and suggests the participation of this neuropeptide in the regulation of gut functions, not only under physiological conditions, but also during pathological processes.
Expression of Progesterone Receptors in Hippocampal Neurons in Rabbit Males
Authors: Krakowska Izabela , Lonc Grzegorz 
Published: February 2013

Abstract
The aim of this study was to investigate the presence of progesterone receptors in hippocampal neurons of male rabbits and the distribution of these receptors in different areas of the hippocampus. The examinations were carried out on brains of five males, New Zealand breed, age of 2 years, weighing 2-3 kg. Immunohistochemical detection of receptors was performed using DAKO LSAB + Kit Peroxidase method. The sections were incubated with primary monoclonal antibody for progesterone receptor NCL-LPGR- AB. The obtained results have shown the presence of progesterone receptors in neurons of CA1, CA3, and CA4 regions of the hippocampus. The reaction also occurred in the region of granular cells of the dental gyrus. The reaction was seen in the cytoplasm and nuclei of neurons simultaneously, whereas some neurons did not show any reaction in the nucleus and cytoplasm. Occurrence of progesterone receptors in most neurons of the hippocampus confirms a direct influence of progesterone on the hippocampus and its functions in rabbit males.
Inhibitory Effect of Epstein-Barr Virus Gene-Ebna1 on Human Tnfrp55 Gene Expression
Authors: Krzowska-Firych Joanna , Fota-Markowska Hanna , Marzec-Kotarska Barbara , Modrzewska Roma , Wojcierowski Jacek 
Published: February 2013

Abstract
The aim of the study was to assess the expression of TNFRp55 mRNA and to examine if the antisense inhibition of Epstein-Barr virus (EBV) encoded EBNA1 gene product alters the expression of gene encoding TNFRp55 in lymphoblastoid cell line (LCL). The experiment was performed on LCL derived from EBV infected human peripheral blood B lymphocytes. The lymphocytes were isolated and cultured. RNA was isolated and examined according to the RNase protection assay. The hybridisation was done with HCR-4 probe. RNA was quantified by densitometry and presented in extinction units. The level of expression was calculated with TotaILab software programme. The results of the study suggest that EBV gene, responsible for the synthesis of EBNA1 protein, has an inhibitory effect on human TNFRp55 gene expression in LCL.
Implications of Blood Coagulation and Fibrinolytic Disorders in Severe Endometritis-Pyometra Complex in Bitches
Authors: Radwińska Justyna , Domosławska Anna , Pomianowski Andrzej , Żarczyńska Katarzyna , Jurczak Andrzej 
Published: February 2013

Abstract
Twenty bitches with acute endometritis-pyometra complex (EPC) and 20 clinically healthy bitches were examined. The following coagulation parameters were determined in haemostatic evaluations: prothrombin time (PT), activated partial thromboplastin time (aPTT), thrombin time (TT), fibrinogen concentrations (FBG), D-dimer concentrations (D-D), antithrombin activity (AT), and blood platelet counts (PLT). Morphological and biochemical blood parameters were also analysed. Examinations of animals affected by EPC revealed blood coagulation and fibrinolytic disorders, and the noted results (PT 13.7 ±1.06 s, aPTT 23.4 ±1.04 s, TT 15.6 ±0.68 s, FBG 2.2 g/L, D-D 785.4 ±103.05 μg/L, AT 111.1 ±13.51%, PLT 169.30 ±126.31 103/μL) point to a high risk of disseminated intravascular coagulation. The findings indicate that the coagulation parameters of bitches affected by EPC should be analysed before treatment as the noted disorder can significantly complicate therapy and ovariohysterectomy, and endanger the patients' life.
Evaluation of Technological Properties and Oxidative Stability of Organic Dry Fermented Probiotic Sausages During Long-Term Storage
Authors: Wójciak Karolina M. , Trząskowska Monika , Kołożyn-Krajewska Danuta , Dolatowski Zbigniew J. 
Published: February 2013

Abstract
The objective of this work was to study the oxidative stability of organic dry fermented probiotic sausages during long-term storage (6 months). Four test samples were prepared: sample A - control sausage, sample B - with 0.05% addition of sodium ascorbate, sample C - with addition of Lb. casei LOCK 0900 (2x106 CFU/g) probiotic strain, 0.6% of glucose, and 0.05% of sodium ascorbate, and sample D - with 0.05% of sodium ascorbate, probiotics, and 0.6% of lactose. The study covered evaluation of the ripening process (21 d) by evaluating loss in the sausage weight. The pH value, oxidation-reduction potential, TBARS values, acid number, peroxide number, browning index after ripening (0) and after 2nd, 4th, and 6th month of chilling storage were identified. The total colour difference of sausages subjected to 3 h exposure to fluorescent light, texture parameters, and count of lactic acid bacteria (LAB) were identified after ripening (0) and after 6 months of storage. It was observed that an addition of probiotic bacteria and glucose (sample C) contributed to a significant decrease in the pH value and water activity in the sausage directly after ripening and during the entire chilling storage period. In samples with probiotics the option with glucose had a lower (P≤0.05) pH value by ca. 0.5 unit as compared to the sample with lactose. Significantly higher (P≤0.05) values of TBARS were observed in samples with probiotics as compared to control samples. The greatest oxidation stability during the entire chilling storage period was found in the sample with sodium ascorbate. Among samples with probiotic strain, the sample with glucose had the lowest peroxide number value (0.58-3.56 meqO2/kg) and TBARS (1.10-2.08 mg MDA/kg) but also the greatest colour stability during exposure to light. The order of decline in oxidative stability was: sample B > sample C > sample D > sample A.
Evaluation of Densitometric and Geometric Parameters of Tibiotarsal Bones in Turkeys
Authors: Charuta Anna , Dzierzęcka Małgorzata , Biesiada-Drzazga Barbara 
Published: February 2013

Abstract
The aim of the study was to analyse densitometric and geometric parameters of healthy and deformed tibiotarsal bones of male turkeys of heavy type with the use of computed tomography. The influence of a bone type (healthy or deformed) and the area of the measurements (the proximal metaphysis and middle of the diaphysis) on these parameters were studied. It was found that the diaphysis of the deformed tibiotarsal bones (45 g) had higher values of the studied parameters than healthy bones (36 g) and they were as follows: BMC - 60.04 mg/mm, vBMD - 549.11 mg/cm3, TRAB_A - 48.34 mm2, CRT_A - 55.57 mm2, PERI_C - 37.55 mm, ENDO_C - 25.42 mm, SSI - 151.19 mm3. The studied parameters were significantly lower in the proximal metaphyses of the deformed tibial bones and amounted to: BMC - 29.48 mg/mm, TRAB_A - 42.22 mm2, CRT_A - 21.44 mm2, PERI_C - 34.64 mm, ENDO_C - 28.06 mm, SSI - 44.87 mm3. On the basis of the conducted research, it was found that significant differences in the densitometric and geometric parameters between the proximal metaphysis and the middle of the diaphysis of the deformed bones may result in deformities and fractures of tibiotarsal bones.
Histopathology of Internal Organs of Farm Animals Fed Genetically Modified Corn and Soybean Meal
Authors: Reichert Michał , Kozaczyński Wojciech , Karpińska Teresa Agnieszka , Bocian Łukasz , Jasik Agnieszka , Kycko Anna , Świątkiewicz Małgorzata , Świątkiewicz Sylwester , Furgał-Dierżuk Iwona , Arczewska-Włosek Anna , Strzetelski Juliusz , Kwiatek Krzysztof 
Published: February 2013

Abstract
Histopathological examination of liver, kidney, spleen, pancreas, duodenum, jejunum, skeletal muscle, and bursa of Fabricius samples, collected from broiler chickens, laying hens, fattening pigs, and calves fed genetically modified corn MON 810 and soybean meal MON-40-3-2 (Roundup Ready, RR), was performed The examination showed no significant differences between the control animals fed diets containing no genetically modified feeds and animals fed genetically modified feeds. In some cases, congestion of parenchyma and focal lymphoid cell infiltrations were observed in all dietary groups, including controls, and therefore, it was assumed that the lesions were not associated with the feeding transgenic feeds.
Neuropathic itch caused by nerve root compression: brachioradial pruritus and notalgia paresthetica / Neuropatski pruritus (svrab) prouzrokovan kompresijom nervnih korenova - brahioradijalni pruritus i nostalgija parestetika
Authors: Berny-Moreno Joanna , Szepietowski Jacek C. 
Published: February 2013

AbstractNeuropathic itch (itching or pruritus) arises from a pathology located at any point along the afferent pathway of the nervous system. It may be related to damage to the peripheral nervous system, such as in postherpetic neuropathy, brachioradial pruritus or notalgia paresthetica. It has many clinical features similar to neuropathic pain. Patients complain of itching, which is associated with burning sensation, aching, and stinging. Brachioradial pruritis (BP) is an intense itching sensation of the arm, usually between the shoulder and elbow of one or both arms. It is an enigmatic condition with a controversial etiology; some authors consider BP to be a photodermatosis, whereas other authors attribute BP to compression of cervical nerve roots. Notalgia paresthetica is an isolated mononeuropathy involving the skin over or near the scapula. Patients have a pruritus on the mid-upper back. The treatment is usually difficult, but capsaicin and local analgesic agents are the options of choice. Brachioradial pruritus and notalgia paresthetica are often unrecognized neurocutaneous conditions and therefore, a thorough history and physical examination are of utmost importance to distinguish symptoms and apply accurate therapeutic options.
Geothermal Water Resources Management – Economic Aspects Of Their Treatment / Gospodarka Zasobami Wód Termalnych - Ekonomiczne Aspekty Ich Uzdatniania
Authors: Tomaszewska Barbara 
Published: February 2013

Streszczenie
Wody geotermalne to źródło czystej energii. Warto więc wykorzystywać je w sposób racjonalny zwłaszcza w sensie energetycznym i gospodarczym. Jednym z kluczowych czynników, determinujących warunki eksploatacji wód geotermalnych, wielkość wykorzystania energii oraz sposób zagospodarowania wód schłodzonych jest zasolenie wód. Podwyższone zasolenie oraz występowanie mikroelementów toksycznych w wodach może często determinować trudności związane utylizacją wykorzystanych wód. Tylko w kilku polskich zakładach geotermalnych wody eksploatowane są w układzie zamkniętym (po wykorzystaniu wtłaczane z powrotem do górotworu). Częściej stosowany jest system otwarty (wody odprowadzane są do cieków powierzchniowych lub kanalizacji) lub mieszany (tylko część wód wraca do złoża za pomocą otworów chłonnych, druga część jest zrzucana do rzek). Odsalanie wód eotermalnych może stanowić, w określonych przypadkach, alternatywę umożliwiającą zaspokojenie lokalnego zapotrzebowania na wodę słodką (np. zdatną do picia).
Ocena możliwości wdrożenia analizowanego procesu na skalę przemysłową w dużej mierze zależy od kierunków i możliwości utylizacji/zagospodarowania koncentratu. Biorąc pod uwagę względy środowiskowe, najkorzystniejszym rozwiązaniem jest wtłaczanie koncentratu do górotworu. Przeprowadzona analiza energe­tyczna i ekonomiczna wykazała, że opłacalność wdrożenia na skalę przemysłową procesu odsalania w systemie geotermalnym w dużej mierze zależy od czynników związanych z jego pracą, a w szczególności: wielkości wydobycia wód geotermalnych, zasolenia wód, parametrów chłonnych otworów przeznaczonych do wtłaczania wód do górotworu, skali problemów związanych z utylizacją schłodzonych wód, lokalnego zapotrzebowania na wody pitne i gospodarcze i in. Kluczowa dla opłacalności tego procesu jest między innymi redukcja wymaganego ciśnienia przy wtłaczaniu wód do górotworu i redukcja wielkości strumienia zatłaczanych wód. Bardzo ważnym elementem jest również zapewnienie odpowiednich warunków zbytu odsolonych wód (cena/ilość) celem pokrycia zapotrzebowania na energię elektryczną wykorzystaną w procesie odsalania.
Dones i ciència. Quatre pioneres en el camp de la psicologia
Authors: Mülberger Annette 
Published: February 2013

Una carrera científica normalment és una carrera d'obstacles, però en el cas de ser una dona s’afegeixen problemes i mecanismes d'exclusió específics. Per tal de conèixer les estratègies utilitzats per superar alguns obstacles, al present article revisem les biografies de quatre psicòlogues que, entre 1890 i 1940, van intentar obtenir una formació acadèmica i van aconseguir fer una contribució pròpia a la ciència psicològica.
Systematic Review and Critical Appraisal of Validation Studies to Identify Rheumatic Diseases in Health Administrative Databases
Authors: Widdifield Jessica , Labrecque Jeremy , Lix Lisa , Paterson J. Michael , Bernatsky Sasha , Tu Karen , Ivers Noah , Bombardier Claire 
Published: February 2013

ObjectiveTo evaluate the quality of the methods and reporting of published studies that validate administrative database algorithms for rheumatic disease case ascertainment.MethodsWe systematically searched MEDLINE, Embase, and the reference lists of articles published from 1980 to 2011. We included studies that validated administrative data algorithms for rheumatic disease case ascertainment using medical record or patient‐reported diagnoses as the reference standard. Each study was evaluated using published standards for the reporting and quality assessment of diagnostic accuracy, which informed the development of a methodologic framework to help critically appraise and guide research in this area.ResultsTwenty‐three studies met the inclusion criteria. Administrative database algorithms to identify cases were most frequently validated against diagnoses in medical records (83%). Almost two‐thirds of the studies (61%) used diagnosis codes in administrative data to identify potential cases and then reviewed medical records to confirm the diagnoses. The remaining studies did the reverse, identifying patients using a reference standard and then testing algorithms to identify cases in administrative data. Many authors (61%) described the patient population, but few (26%) reported key measures of diagnostic accuracy (sensitivity, specificity, and positive and negative predictive values). Only one‐third of studies reported disease prevalence in the validation study sample.ConclusionThe methods used in administrative data validation studies of rheumatic diseases are highly variable. Few studies reported key measures of diagnostic accuracy despite their importance for drawing conclusions about the validity of administrative database algorithms. We developed a methodologic framework and recommendations for validation study conduct and reporting.
Prevalence of Axial Spondyloarthritis in United States Rheumatology Practices: Assessment of SpondyloArthritis International Society Criteria Versus Rheumatology Expert Clinical Diagnosis
Authors: Strand Vibeke , Rao Sumati A. , Shillington Alicia C. , Cifaldi Mary A. , Mcguire Michael , Ruderman Eric M. 
Published: February 2013

ObjectiveNew classification criteria for axial spondyloarthritis (SpA) have been validated by the Assessment of SpondyloArthritis international Society (ASAS) working group. We applied these criteria to estimate prevalence of SpA in randomly selected, retrospectively reviewed medical records from representative US rheumatology practices.MethodsRheumatologists from 101 US practices identified at‐risk patients, ages 18–44 years, with chronic back pain. Medical records were reviewed against ASAS criteria. The proportion of patients meeting ASAS criteria was compared to an estimate of the total number of at‐risk patients treated at participating sites and, following weighting, was extrapolated to 5,520 US rheumatology practices. US Census data were used to estimate national prevalence.ResultsIn a sample of 816 randomly selected records, 514 (63%) at‐risk patients (95% confidence interval [95% CI] 59.6–66.3%) met ASAS criteria. By applying this proportion to 1,217,097 Americans estimated at risk, 766,652 were projected to meet ASAS criteria. This projection corresponds to a national prevalence of 0.70% (95% CI 0.38–1.1%) or 701 per 100,000 individuals. The prevalence estimates of ankylosing spondylitis and nonradiographic axial SpA are 0.35% (95% CI 0.18–0.554%) and 0.35% (95% CI 0.18–0.554%), respectively. Rheumatologists diagnosed axial SpA in 491 (60%) of those at risk, corresponding to 0.67% (95% CI 0.36–1.01%) prevalence overall. However, of 514 patients meeting ASAS criteria, 124 (24%) were undiagnosed by rheumatologists.ConclusionThis is the first systematic epidemiology study of axial SpA using ASAS criteria. Better recognition of axial symptoms is needed, as rheumatologists' expert clinical diagnoses are not always in agreement with ASAS criteria.
Brief Report: CD109 Overexpression Ameliorates Skin Fibrosis in a Mouse Model of Bleomycin‐Induced Scleroderma
Authors: Vorstenbosch Joshua , Al‐Ajmi Hasan , Winocour Sebastian , Trzeciak Alissa , Lessard Lucie , Philip Anie 
Published: February 2013

AbstractObjectiveTransforming growth factor β (TGFβ) is a profibrotic cytokine, and its aberrant function is implicated in several types of fibrotic pathologies including scleroderma (systemic sclerosis [SSc]). Multiple lines of evidence show that increased TGFβ signaling contributes to progressive fibrosis in SSc by promoting fibroblast activation, excessive extracellular matrix (ECM) deposition, and dermal thickening. We have previously identified CD109 as a TGFβ coreceptor and have shown that it antagonizes TGFβ signaling and TGFβ‐induced ECM expression in vitro in human keratinocytes and fibroblasts. The aim of the present study was to examine the ability of CD109 to prevent skin fibrosis in a mouse model of bleomycin‐induced SSc.MethodsTransgenic mice overexpressing CD109 in the epidermis and their wild‐type (WT) littermates were injected with bleomycin in phosphate buffered saline (PBS) or with PBS alone every other day for 21 days or 28 days. Dermal thickness and collagen deposition were determined histologically using Masson's trichrome and picrosirius red staining. In addition, collagen and fibronectin content was analyzed using Western blotting, and activation of TGFβ signaling was examined by determining phospho‐Smad2 and phospho‐Smad3 levels using Western blotting and immunohistochemistry.ResultsTransgenic mice overexpressing CD109 in the epidermis showed resistance to bleomycin‐induced skin fibrosis, as evidenced by a significant decrease in dermal thickness, collagen crosslinking, collagen and fibronectin content, and phospho‐Smad2/3 levels, as compared to their WT littermates.ConclusionOur findings suggest that CD109 inhibits TGFβ signaling and fibrotic responses in experimental murine scleroderma. They also suggest that CD109 regulates dermal–epidermal interactions to decrease extracellular matrix synthesis in the dermis. Thus, CD109 is a potential molecular target for therapeutic intervention in scleroderma.