bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pan-histone deacetylase inhibitors regulate signaling pathways involved in proliferative and pro-inflammatory mechanisms in H9c2 cells
Authors: Majumdar Gipsy , Adris Piyatilake , Bhargava Neha , Chen Hao , Raghow Rajendra 
Published: December 2012

Abstract

Background
We have shown previously that pan-HDAC inhibitors (HDACIs) m-carboxycinnamic acid bis-hydroxamide (CBHA) and trichostatin A (TSA) attenuated cardiac hypertrophy in BALB/c mice by inducing hyper-acetylation of cardiac chromatin that was accompanied by suppression of pro-inflammatory gene networks. However, it was not feasible to determine the precise contribution of the myocytes- and non-myocytes to HDACI-induced gene expression in the intact heart. Therefore, the current study was undertaken with a primary goal of elucidating temporal changes in the transcriptomes of cardiac myocytes exposed to CBHA and TSA.

Results
We incubated H9c2 cardiac myocytes in growth medium containing either of the two HDACIs for 6h and 24h and analyzed changes in gene expression using Illumina microarrays. H9c2 cells exposed to TSA for 6h and 24h led to differential expression of 468 and 231 genes, respectively. In contrast, cardiac myocytes incubated with CBHA for 6h and 24h elicited differential expression of 768 and 999 genes, respectively. We analyzed CBHA- and TSA-induced differentially expressed genes by Ingenuity Pathway (IPA), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Core_TF programs and discovered that CBHA and TSA impinged on several common gene networks. Thus, both HDACIs induced a repertoire of signaling kinases (PTEN-PI3K-AKT and MAPK) and transcription factors (Myc, p53, NFkB and HNF4A) representing canonical TGFβ, TNF-α, IFNγ and IL-6 specific networks. An overrepresentation of E2F, AP2, EGR1 and SP1 specific motifs was also found in the promoters of the differentially expressed genes. Apparently, TSA elicited predominantly TGFβ- and TNF-α-intensive gene networks regardless of the duration of treatment. In contrast, CBHA elicited TNF-α and IFNγ specific networks at 6 h, followed by elicitation of IL-6 and IFNγ-centered gene networks at 24h.

Conclusions
Our data show that both CBHA and TSA induced similar, but not identical, time-dependent, gene networks in H9c2 cardiac myocytes. Initially, both HDACIs impinged on numerous genes associated with adipokine signaling, intracellular metabolism and energetics, and cell cycle. A continued exposure to either CBHA or TSA led to the emergence of a number of apoptosis- and inflammation-specific gene networks that were apparently suppressed by both HDACIs. Based on these data we posit that the anti-inflammatory and anti-proliferative actions of HDACIs are myocyte-intrinsic. These findings advance our understanding of the mechanisms of actions of HDACIs on cardiac myocytes and reveal potential signaling pathways that may be targeted therapeutically.
Characteristics of a broad lytic spectrum endolysin from phage BtCS33 of Bacillus thuringiensis
Authors: Yuan Yihui , Peng Qin , Gao Meiying 
Published: December 2012

Abstract

Background
Endolysins produced by bacteriophages lyse bacteria, and are thus considered a novel type of antimicrobial agent. Several endolysins from Bacillus phages or prophages have previously been characterized and used to target Bacillus strains that cause disease in animals and humans. B. thuringiensis phage BtCS33 is a Siphoviridae family phage and its genome has been sequenced and analyzed. In the BtCS33 genome, orf18 was found to encode an endolysin protein (PlyBt33).

Results
Bioinformatic analyses showed that endolysin PlyBt33 was composed of two functional domains, the N-terminal catalytic domain and the C-terminal cell wall binding domain. In this study, the entire endolysin PlyBt33, and both the N- and C-termini,were expressed in Escherichia coli and then purified. The lytic activities of PlyBt33 and its N-terminus were tested on bacteria. Both regions exhibited lytic activity, although PlyBt33 showed a higher lytic activity than the N-terminus. PlyBt33 exhibited activity against all Bacillus strains tested from five different species, but was not active against Gram-negative bacteria. Optimal conditions for PlyBt33 reactivity were pH 9.0 and 50°C. PlyBt33 showed high thermostability, with 40% of initial activity remaining following 1 h of treatment at 60°C. The C-terminus of PlyBt33 bound to B. thuringiensis strain HD-73 and Bacillus subtilis strain 168. This cell wall binding domain might be novel, as its amino acid sequence showed little similarity to previously reported endolysins.

Conclusions
PlyBt33 showed potential as a novel antimicrobial agent at a relatively high temperature and had a broad lytic spectrum within the Bacillus genus. The C-terminus of PlyBt33 might be a novel kind of cell wall binding domain.
The role of brevican in glioma: promoting tumor cell motility in vitro and in vivo
Authors: Lu Renquan , Wu Chengsheng , Guo Lin , Liu Yingchao , Mo Wei , Wang Huijie , Ding Jianbo , Wong Eric T , Yu Min 
Published: December 2012

Abstract

Background
Malignant glioma is a common primary tumor of the central nervous system. Brevican, an abundant extracellular matrix component in the adult brain, plays a critical role in the process of glioma. The mechanisms for the highly invasive behavior of gliomas are still poorly understood. The aim of this study was to examine whether brevican is a predictor of glioma and its roles in glioma cell motility.

Methods
In this study, immunohistochemistry staining for brevican expression was performed in malignant gliomas and benign controls. We also explored the effects of brevican on cell adhesion and migration in brevican-overexpressed cells. Knockdown of brevican expression was achieved by stable transfection of U251 cells transduced with a construct encoding a short hairpin DNA directed against the brevican gene, which correspondingly, down-regulated the proliferation, invasion and spread of brevican-expressing cells. Moreover, the role of brevican in the growth and progression of glioma was demonstrated by in vivo studies.

Results
Our results provide evidence for the molecular and cellular mechanisms that may underlie the motility-promoting role of brevican in the progression of glioma. The role of brevican as a target for immunotherapy might be taken into consideration in future studies.

Conclusions
This study suggests that expression of brevican is associated with glioma cell adhesion, motility and tumor growth, and also is related to glioma cell differentiation, therefore it may be a marker for malignance degree of glioma
Molecular pathways undergoing dramatic transcriptomic changes during tumor development in the human colon
Authors: Maglietta Rosalia , Liuzzi Vania Cosma , Cattaneo Elisa , Laczko Endre , Piepoli Ada , Panza Anna , Carella Massimo , Palumbo Orazio , Staiano Teresa , Buffoli Federico , Andriulli Angelo , Marra Giancarlo , Ancona Nicola 
Published: December 2012

Abstract

Background
The malignant transformation of precancerous colorectal lesions involves progressive alterations at both the molecular and morphologic levels, the latter consisting of increases in size and in the degree of cellular atypia. Analyzing preinvasive tumors of different sizes can therefore shed light on the sequence of these alterations.

Methods
We used a molecular pathway-based approach to analyze transcriptomic profiles of 59 colorectal tumors representing early and late preinvasive stages and the invasive stage of tumorigenesis. Random set analysis was used to identify biological pathways enriched for genes differentially regulated in tumors (compared with 59 samples of normal mucosa).

Results
Of the 880 canonical pathways we investigated, 112 displayed significant tumor-related upregulation or downregulation at one or more stages of tumorigenesis. This allowed us to distinguish between pathways whose dysregulation is probably necessary throughout tumorigenesis and those whose involvement specifically drives progression from one stage to the next. We were also able to pinpoint specific changes within each gene set that seem to play key roles at each transition. The early preinvasive stage was characterized by cell-cycle checkpoint activation triggered by DNA replication stress and dramatic downregulation of basic transmembrane signaling processes that maintain epithelial/stromal homeostasis in the normal mucosa. In late preinvasive lesions, there was also downregulation of signal transduction pathways (e.g., those mediated by G proteins and nuclear hormone receptors) involved in cell differentiation and upregulation of pathways governing nuclear envelope dynamics and the G2>M transition in the cell cycle. The main features of the invasive stage were activation of the G1>S transition in the cell cycle, upregulated expression of tumor-promoting microenvironmental factors, and profound dysregulation of metabolic pathways (e.g., increased aerobic glycolysis, downregulation of pathways that metabolize drugs and xenobiotics).

Conclusions
Our analysis revealed specific pathways whose dysregulation might play a role in each transition of the transformation process. This is the first study in which such an approach has been used to gain further insights into colorectal tumorigenesis. Therefore, these data provide a launchpad for further exploration of the molecular characterization of colorectal tumorigenesis using systems biology approaches.
Early infections are associated with increased risk for celiac disease: an incident case-referent study
Authors: Myléus Anna , Hernell Olle , Gothefors Leif , Hammarström Marie-Louise , Persson Lars-Åke , Stenlund Hans , Ivarsson Anneli 
Published: December 2012

Abstract

Background
Celiac disease is defined as a ‘chronic small intestinal immune-mediated enteropathy precipitated by exposure to dietary gluten in genetically predisposed individuals’. Sweden has experienced an “epidemic” of celiac disease in children below two years of age. Celiac disease etiology is considered multifactorial; however, little is known regarding potential risk- or protecting factors. We present data on the possible association between early infectious episodes and celiac disease, including their possible contribution to the Swedish celiac disease epidemic.

Methods
A population-based incident case-referent study (475 cases, 950 referents) with exposure information obtained via a questionnaire (including family characteristics, infant feeding, and the child’s general health) was performed. Celiac disease cases were diagnosed before two years of age, fulfilling the diagnostic criteria of the European Society for Pediatric Gastroenterology, Hepatology and Nutrition. Referents were randomly selected from the national population register after fulfilling matching criteria. The final analyses included 954 children, 373 (79%) cases and 581 (61%) referents, with complete information on main variables of interest in a matched set of one case with one or two referents.

Results
Having three or more parental-reported infectious episodes, regardless of type of infection, during the first six months of life was associated with a significantly increased risk for later celiac disease, and this remained after adjusting for infant feeding and socioeconomic status (odds ratio [OR] 1.5; 95% confidence interval [CI], 1.1-2.0; P=0.014). The celiac disease risk increased synergistically if, in addition to having several infectious episodes, infants were introduced to dietary gluten in large amounts, compared to small or medium amounts, after breastfeeding was discontinued (OR 5.6; 95% CI, 3.1-10; P<0.001).

Conclusion
This study suggests that having repeated infectious episodes early in life increases the risk for later celiac disease. In addition, we found a synergistic effect between early infections and daily amount of gluten intake, more pronounced among infants for whom breastfeeding had been discontinued prior to gluten introduction. Regarding contribution to the Swedish celiac disease epidemic, which partly was attributed to concurrent changes in infant feeding, early infections probably made a minor contribution via the synergistic effect with gluten amount.
Does IFN-γ play a role on the pathogenesis of non-atopic asthma in Latin America children?
Authors: Figueiredo Camila Alexandrina , Rodrigues Laura Cunha , Alcantara-Neves Neuza Maria , Cooper Philip J , Amorim Leila Denise , Silva Nivea Bispo , Cruz Alvaro A , Barreto Mauricio Lima 
Published: December 2012

Abstract
In this work we explore differences in blood cells and cytokine profiles in children according to atopic status and asthma (atopic or non-atopic). The study involved measurement of Th1(IFN-γ) and Th2 (IL-5 and IL-13) cytokines in Dermatophagoides pteronyssinus stimulated peripheral blood leukocytes, blood cell count, skin prick test and specific IgE against common aeroallergens. Atopic status was associated with eosinophilia and production of Th2 type cytokines. Atopic asthma was associated with eosinophilia and non-atopic asthma was associated with IFN-γ and elevated monocytes in blood. IFN-γ and monocytes might play a role in immunopathology of non-atopic asthma in Latin American children.
Upregulation of Cystathionine Beta-Synthetase Expression by Nuclear Factor-Kappa B Activation Contributes to Visceral Hypersensitivity in Adult Rats with Neonatal Maternal Deprivation
Authors: Li Lin , Xie Ruihua , Hu Shufen , Wang Yongmeng , Yu Tianzhu , Xiao Ying , Jiang Xinghong , Gu Jianguo , Hu Chuang-Ying , Xu Guang-Yin 
Published: December 2012

Background: Irritable bowel syndrome (IBS) is characterized by chronic visceral hyperalgesia (CVH) that manifested with persistent or recurrent abdominal pain and altered bowel movement. However, the pathogenesis of the CVH remains unknown. The aim of this study was to investigate roles of endogenous hydrogen sulfide (H2S) producing enzyme cystathionine beta-synthetase (CBS) and p65 nuclear factor-kappa B subunits in CVH. Results: CVH was induced by neonatal maternal deprivation (NMD) in male rats on postnatal days 2–15 and behavioral experiments were conducted at the age of 7–15 weeks. NMD significantly increased expression of CBS in colon-innervating DRGs from the 7th to 12th week. This change in CBS express is well correlated with the time course of enhanced visceromoter responses to colorectal distention (CRD), an indicator of visceral pain. Administration of AOAA, an inhibitor of CBS, produced a dose-dependent antinociceptive effect on NMD rats while it had no effect on age-matched healthy control rats. AOAA also reversed the enhanced neuronal excitability seen in colon-innervating DRGs. Application of NaHS, a donor of H2S, increased excitability of colon-innervating DRG neurons acutely dissociated from healthy control rats. Intrathecal injection of NaHS produced an acute visceral hyperalgesia. In addition, the content of p65 in nucleus was remarkably higher in NMD rats than that in age-matched controls. Intrathecal administration of PDTC, an inhibitor of p65, markedly reduced expression of CBS and attenuated nociceptive responses to CRD. Conclusion: The present results suggested that upregulation of CBS expression, which is mediated by activation of p65, contributes to NMD-induced CVH. This pathway might be a potential target for relieving CVH in patients with IBS.
The participation of Wajãpi women from the State of Amapá (Brazil) in the traditional use of medicinal plants – a case study
Authors: da Mata Nely Dayse Santos , de Sousa Rosinaldo Silva , Perazzo Fábio F , Carvalho José Carlos Tavares 
Published: December 2012

Abstract

Background
The purpose of this study was to analyze the importance of traditional medicinal plants use to Wajãpi women in the State of Amapá, Brazil, as well as their practices in the local common illnesses of treatment considering the prevailing practice by non-Indians.

Methods
This study was conducted in the Community of the Wajãpi Indigenous People, a Brazilian territory located in the central western State of Amapá. Wajãpi women were selected for the interview since they have the responsibility to harvest, collect and prepare the preparations. The studied women were residents of four villages. The number of women within these four villages is 24.

Results and conclusions
The findings fell into the following three categories: 1) The daily use of medicinal plants by women and main methods of application. In this category, the botanical families found included Leguminosae-Caesalpinoideae, Anacardiaceae, Meliaceae, and Rubiaceae. The main forms of use found were teas, baths, maceration, in natura, and juices; 2) Through analysis of illness and treatment records, a lack of knowledge integration in the health system was shown to be due to a variety of gaps and the need of health professionals to be more aware about the local culture which they intend to work with, what could decrease the prevailing barriers between the social groups involved; 3) Traditional knowledge and possible sustainability can be fostered by stimulating the transmission of traditional knowledge from generation to generation, therefore reducing the dependence on industrialized medicines and also by maintaining an appreciation of those practices among youngsters, who tend to question them.
The combination of stem cell factor and granulocyte-colony stimulating factor for chronic stroke treatment in aged animals
Authors: Piao Chun-Shu , Gonzalez-Toledo Maria E , Gu Xi , Zhao Li-Ru 
Published: December 2012

Abstract

Background
Stroke occurs more frequently in the elderly population and presents the number one leading cause of persistent disability worldwide. Lack of effective treatment to enhance brain repair and improve functional restoration in chronic stroke, the recovery phase of stroke, is a challenging medical problem to be solved in stroke research. Our early study has revealed the therapeutic effects of stem cell factor (SCF) in combination with granulocyte-colony stimulating factor (G-CSF) (SCF+G-CSF) on chronic stroke in young animals. However, whether this treatment is effective and safe to the aged population remains to be determined.

Methods
Cortical brain ischemia was produced in aged C57BL mice or aged spontaneously hypertensive rats. SCF+G-CSF or equal volume of vehicle solution was subcutaneously injected for 7 days beginning at 3–4 months after induction of cortical brain ischemia. Using the approaches of biochemistry assays, flow cytometry, pathology, and evaluation of functional outcome, several doses of SCF+G-CSF have been examined for their safety and efficiency on chronic stroke in aged animals.

Results
All tested doses did not show acute or chronic toxicity in the aged animals. Additionally, SCF+G-CSF treatment in chronic stroke of aged animals mobilized bone marrow stem cells and improved functional outcome in a dose-dependent manner.

Conclusions
SCF+G-CSF treatment is a safe and effective approach to chronic stroke in the aged condition. This study provides important information needed for developing a new therapeutic strategy to improve the health of older adults with chronic stroke.
Regeneration of Caramel Saturated Activated Carbon jointly by Microwave and Extractive Method
Authors: Lv Guocheng , Wu Limei , Wang Xiaoyu , Liao Libing , Li Zhaohui , He Wenhui 
Published: December 2012

Abstract
In this study, the spent activated carbon (AC) saturated with caramel was regenerated using solvent assisted with microwave radiation. The efficiency of regeneration was evaluated under parameters such as power and time of microwave radiation, and concentrations and pH of an activator. The quality of regenerated AC (RAC) was assessed by methylene blue (MB) and iodine adsorption. The optimum condition for AC regeneration was under a microwave power of 680 W and 10 min radiation, with 20% ethyl alcohol in the activator and pH of 9–10. Under this condition, the adsorption of iodine and MB on RAC was 878 and 218 mg/g, respectively. The micro structure of RAC was characterized by SEM observation. The surface area of RAC was determined under N2 sorption. The pore size distributions of virgin and RAC determined by Horvath–Kawazoe (HK) and DFT methods resulted in a mean pore diameter of 1.2 nm and a pore volume of 1 cm3/g. A regeneration efficiency of more than 85% could be achieved by microwave assisted solvent regeneration.
On the Relationship between Tariff Levels and the Nature of Mergers
Authors: Ulus Aysegul , Yildiz Halis M. 
Published: December 2012

Abstract
This paper employs an endogenous merger formation approach in a two-country oligopoly model of trade to examine the international linkages between the nature of mergers and tariff levels. Firms sell differentiated products and compete in a Bertrand fashion in product markets. Two effects play key roles in determining equilibrium market structure: the tariff saving effect and the protection gain effect. The balance between these two effects implies that, when foreign country practices free trade, low home tariffs yield international mergers irrespective of the substitutability levels. By contrast, when foreign tariffs are sufficiently high and products are close substitutes, national mergers obtain in the equilibrium. Unlike this asymmetric result of unilateral trade liberalization, we find that when bilateral tariffs are sufficiently low, international mergers arise. These results fit well with the fact that global trade liberalization has been accompanied by an increase in international merger activities. From a welfare perspective, we show that international mergers are preferable to national mergers and thus social and private merger incentives become aligned together as trade gets bilaterally liberalized. Finally, if countries can commit to a trade policy, they would optimally commit to a low tariff to induce international mergers when products are close substitutes while any tariff commitment is optimal when products are sufficiently differentiated.
Sonographic presentations of uterine rupture following vaginal birth after cesarean – report of two cases 12 h apart
Authors: Weissmann-Brenner Alina , Gilboa Yinon , Yerushalmi Gil M. , Achiron Reuven , Schiff Eyal 
Published: December 2012

Abstract
We present two patients who delivered vaginally following a prior cesarean section in whom uterine rupture was detected a few minutes after delivery. Both deliveries occurred within 12 h. The sonographic findings were different in each case and consistent with the clinical and operative findings. Ultrasound may assist in the diagnosis of uterine rupture following delivery.
Prenatal diagnosis of thrombocytopenia-absent radius syndrome
Authors: Köken Gülengül , Akbaş Pınar , Coşar Emine 
Published: December 2012

Abstract
The prenatal diagnosis of thrombocytopenia-absent radius (TAR) syndrome by ultrasound is established. The sonographic findings showed bilateral absence of the radii and club hands with normal thumbs and metacarpals. Thrombocytopenia was identified from the postabortal cord blood. Three-dimensional computerized tomography images confirmed the sonographic diagnosis. This report, to our knowledge, is one of the few cases published in the literature about the prenatal diagnosis of TAR syndrome.