bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of the Effectiveness of Cholera Bacteriophages for Prevention of Experimental Cholera
Authors: Tyurina A. V. , Gaevskaya N. E. , Ivanova I. A. , Filippenko A. V. , Omel’chenko N. D. , Trufanova A. A. , Pogozhova M. P. , Anoprienko A. O. , Sizova Yu. V. , Pasyukova N. I. 
Published: July 2024

The possibility of cholera importation into our country and the increase in the number of Vibrio cholerae strains that are resistant to antibacterial agents necessitate the development of alternative therapeutic and prophylactic biological products based on bacteriophages.The aim of the work was to study the effectiveness of application of cholera bacteriophages for the prevention of experimental cholera.Materials and methods. The work involved cholera bacteriophages Rostov-M3, Rostov-13, active against cholera vibrios of the O1 serogroup; and FB1, which has lytic activity against the O139 serogroup. The effectiveness of cholera prevention was assessed using a model of an isolated loop of the small intestine in an adult rabbit.Results and discussion. The use of Rostov-M3 and Rostov-13 for five and especially seven days before infection with virulent strains of V. cholerae O1 serogroup prevents the development of infection in the small intestine of experimental animals. Bacteriophage FB1 did not have that ability against V. cholerae O139. These studies indicate the effectiveness of using phages Rostov-M3 and Rostov-13 for the prevention of experimental cholera caused by V. cholerae O1 serogroup.
Sustainable regulation of calcium magnesium phosphate and rapeseed cake on soil-tea system in Mount Lushan, China
Authors: Han Chao , Ding Yuan , Lai YuQi , Tang ZiJin , Liu Min 
Published: July 2024

Abstract

Lushan Yunwu tea quality is limited by soil acidity and sterility. This article examined a three-year localization experiment at 1100 m altitude to demonstrate the sustainable management of conditioners, calcium magnesium phosphate (P), rapeseed cake (C), and combination application (P + C) by one-time application on the soil-tea system in Mount Lushan. The study found that conditioners (P, C, P + C) reduced soil acidification and maintained a pH of 4.75–5.34, ideal for tea tree development for three years. Phosphorus activation coefficient (PAC), nitrogen activation coefficient (NAC), and organic matter (OM) content were significantly higher (P < 0.05) in the first year after conditioner treatment, with P + C being the best. After P + C, PAC, NAC, and OM rose by 31.25%, 47.70%, and 10.06 g·kg-1 compared to CK. In comparison to the CK, tea's hundred-bud weight (BW), free amino acids (AA), tea polyphenols (TPC), and chlorophyll (Chl) content of P + C treatment got 29.98%, 14.41%, 22.49%, and 28.85% increase compared to that of the CK, respectively. In the second year, the three treatments of P, C and P + C still had significant moderating effects on the physicochemical properties of the soil and the quality indexes of the tea leaves. The PAC of the soil under the three treatments increased by 0.06%, 0.07% and 0.18%, respectively, as compared to the control.P + C increased BW, AA, TPC and Chl of tea for two years. Three conditioners had two-year regulatory impacts on soil fertility indicators, tea output, and quality. C and P + C both increased soil OM by 18.59% and 21.78% compared to CK in the third year, outperforming P treatment. Redundancy analysis revealed that the primary physicochemical factors influencing tea output and quality were soil OM and pH, with available phosphorus, urease, acid phosphatase, and available nitrogen following closely afterwards.
Method of lithology identification in carbonates reservoirs using well logs based on deep forest
Authors: Kaijun Tong , Sun Futing , Dong Shaoqun , Ji Chunqiu , Zeng Lianbo , Wang Leting , Liu Yuanyuan , Zhang Fuyu 
Published: July 2024

Abstract

Lithology identification of complex carbonate reservoirs is very important for fine characterization and quantitative evaluation of reservoirs. In order to solve the problem of complex carbonate reservoir lithologic logging response with strong multi solutions, this paper introduces the deep forest algorithm to deeply mine lithologic information from the conventional logging calibrated by core descriptions, so as to improve the lithology identification accuracy of complex carbonate reservoir. Deep forest is a combination of random forest algorithm and neural network. It avoids the shortage of long training time of deep neural network through the cascade structure of feedforward and can automatically adjust the model structure. Taking the complex carbonate reservoir of Paleogene Oligocene-Neogene Miocene AS formation in B oilfield of Zagros basin in the Middle East as an example, the deep forest is used to identify and analyze 8 kinds of lithology. On the basis of sensitivity analysis of lithologic logging curves, 9 curves such as gamma ray, compensative density and photoelectric absorption cross-section index are selected to build a lithology identification model based on deep forest. The results of the posterior well analysis show that the deep forest algorithm is better than the traditional random forest algorithm for thin layer lithology and transition lithology identification, and the lithology identification accuracy is 94.4%. The influence of the number of training samples and the setting of model parameters on the lithology identification results shows that the deep forest method can improve nearly up to 2% compared with the random forest method under different training samples, and its default parameters can achieve a lithology identification accuracy of more than 91%.
Courtship Behavior of Adult Spodoptera frugiperda (Lepidoptera: Noctuidae) Observed Using Trackit 3D Trajectory Tracking
Authors: Liu Jie , Mariam Tallat , Wangi Gensong , Li Zhi , Li Guoping , Feng Hongqiang 
Published: July 2024

Abstract

Spodoptera frugiperda, also known as the fall armyworm, is classified by the Food and Agriculture Organization of the United Nations (FAO) as a major agricultural pest. By gaining a more nuanced understanding of the fall armyworm's courtship behavior, simpler and more environmentally friendly methods of controlling this pest can be developed. This study used the Trackit 3D system to meticulously record and describe the activity characteristics and patterns of adult males and females during courtship. Results showed that adult fall armyworms engaged in a variety of activities during courtship that were either singular (flying, flapping, moving, crawling), continuous (flapping + flying, flapping + crawling, flying + crawling), or interspersed (flapping + touching + flapping, flying + touching + flying). Flying and flapping were the most common activities, with observed flight patterns consisting of parabolic, circular, and zigzag trajectories. adult fall armyworms use wing vibration as a means to attract or repel potential mates. In addition, both male and female adults actively search for host plants during courtship, providing a theoretical basis for the future use of plant volatiles to attract and control adult populations. In addition to adult males, adult female fall armyworms were also observed to be highly active during courtship, suggesting that they play a proactive role in mate seeking, providing an opportunity to attract adult females with male pheromone attractants. Peak activity times for adult fall armyworms are mainly concentrated at 11:00 p.m., 3:00 a.m. and 5:00 a.m., providing fundamental data for precise attraction and control of adult fall armyworms at later stages.
TRIM28 regulates the coagulation cascade inhibited by p72 of African swine fever virus
Authors: Zhu Xuejiao , Li Fang , Fan Baochao , Zhao Yongxiang , Zhou Junming , Wang Dandan , Liu Renqiang , Zhao Dongming , Fan Huiying , Li Bin 
Published: July 2024

Abstract

In 2018, African swine fever virus (ASFV) emerged in China, causing extremely serious economic losses to the domestic pig industry. Infection with ASFV can cause disseminated coagulation, leading to the consumption of platelets and coagulation factors and severe bleeding. However, the mechanism of virus-induced coagulation has yet to be established. In our study, ASFV downregulated the coagulation process, as detected by D-dimer (D2D) and Factor X (F10) expression in pigs challenged with ASFV HLJ/18. In vitro, ASFV infection increased Factor IX (F9) and Factor XII (F12) expression while downregulating F10 expression in porcine alveolar macrophages (PAMs). African swine fever virus induced both intrinsic and extrinsic coagulation cascades. In addition, several encoded proteins have been proven to affect the expression of the crucial coagulation protein F10, and among the encoded proteins, p72 inhibits the activity and expression of F10. Proteomicanalysis also revealedthat p72 isinvolved in the coagulation cascade. p72 can interact with F10, and its inhibitory functional domains include amino acids 423-432 and amino acids 443−452. Finally, we found that F10 and p72 interact with tripartite motif-containing protein 28 (TRIM28). TRIM28 knockdown resulted in a decrease in F10 expression. Importantly, TRIM28 contributes to the reduction in F10 protein expression regulated by p72. Our findings revealed an inhibitory effect of the viral protein p72 on the ASFV infection-induced coagulation cascade and a role of TRIM28 in reducing F10 expression, revealing a molecular mechanism of ASFV-associated coagulation.
Delay in hospital arrival and determinate factors among acute stroke Patients at Yekatit-12 Hospital Medical Collage, Ethiopia: Unmatched case-control study
Authors: Mamo Biniyam Tedla , Tefera Dawit Bekele , Altaye Makeda Girma , Geram Ferehiwot Gebrehiwot , Dano Anteneh Mitiku , Sana Yeteshaw Bekele , Gebre Feven Sahle , Bonger Zelalem Tazu 
Published: July 2024

Abstract

Background: Stroke is a rapidly developing clinical sign of focal or global disturbance of cerebral function, lasting more than 24 hours or leading to death with no apparent cause other than that of vascular origin. prehospital delay in stroke is the time between the onset of symptoms to admission to the Hospital.
Objective: To determine factors associated with delayed arrival of an adult at the hospital following an acute stroke
Method:An unmatched case–control study was conducted from September 2021 to November 2023 at Yekatit 12 Hospital Medical College. Descriptive statistics were used to describe the study variables. Bivariable and multivariable logistic regression analyses were used to identify the determinants of delayed presentation of stroke to the hospital.
Result: Out of 83 cases (presenting after four hours) and 166 controls (presenting within four or fewer hours) of an acute stroke patient who was admitted to the emergency department; The duration of symptoms from the onset of stroke to presentation to the hospital, the median time for cases was 24 hours (IQR, 12 hr) and in control the median time was 2 hours. The study found Age 60 or less (AOR 1.75, 95% CI 1–3.09, p<0.05), living outside of Addis Ababa (AOR 3.55, 95% CI 1.33-9.46, p<0.011), onset of stroke occurring at night (AOR 1.75, 95% CI 1–3.1, p<0.05) and not having health insurance (AOR 0.43, 95% CI 0.23- 0.8,p< 0.007) were identified as predicting factors of late presentation in acute stroke patients.
Conclusion: This study highlights key factors affecting delayed presentation of acute stroke patients. Specifically, age, place of residence, health insurance, and stroke onset time significantly influence the timeliness of seeking medical care. Improving the modifiable factors through public education can enhance early thrombolytic action, improve the overall prognosis of stroke patients, and avoid delays in the early hospital admission of these patients.
Sparrow Search Algorithm Optimized LSTM Model for CSI-Based Indoor Localization
Authors: Wang Yan , Zhou Yuqing 
Published: July 2024

Abstract

In indoor positioning, Channel State Information (CSI) holds significant importance. CSI provides detailed channel characteristics, including amplitude and phase information, enabling the positioning system to estimate device locations more accurately. Compared to traditional RSSI methods, CSI offers higher positioning accuracy and better interference resistance. Additionally, it supports various advanced positioning algorithms, making it suitable for complex indoor environments and enabling real-time precise positioning. In this study, a localization method based on CSI phase and amplitude data is proposed with the aim of improving the accuracy and robustness of indoor localization. In the offline training phase, for each reference point, we construct a new dataset using pre-processed amplitude and phase data, which are transformed into image data for pre-training to extract features. Subsequently, a CNN-LSTM neural network with an integrated attention mechanism is trained to capture complex spatio-temporal relationships in the data. However, manual tuning of neural network hyperparameters has its limitations. Therefore, this paper introduces the Sparrow Search Algorithm (SSA)bio-inspired algorithm to optimize the combination of hyperparameters, aiming to obtain a more optimal model performance. During the online orientation phase, CSI data collected from the target device is processed using the same steps as in the offline phase to convert it into images. The trained model is then used to perform regression predictions on test points, enabling localization of the target position. To assess the effectiveness of our proposed approach, we ran tests in both open and complicated laboratory settings. Comparisons were made with several existing indoor localization solutions (such as PCNB, MIMO, LSTM, and FIFS), demonstrating that our approach exhibits significant advantages regarding localization accuracy and robustness.
Targeted Gene-Hormone Therapy of Colorectal Cancer with Guanylin Expressing Nano-system: In Silico and In Vitro Study
Authors: Samadi Pouria , Rahbarizadeh Fatemeh , Nouri Fatemeh , Soleimani Meysam , Najafi Rezvan , Jalali Akram 
Published: July 2024

Abstract

Background: Addressing colorectal cancer (CRC) poses a significant challenge, demanding the precise delivery of therapeutic agents to eliminate cancer cells while minimizing the impact on healthy cells. The strategic selection of therapeutic targets, the utilization of nanocarriers with optimal efficacy and low toxicity, and the development of gene constructs with targeted expression in cancer cells are crucial aspects of this pursuit.
Materials and Methods: This study employed a systems biology approach to comprehensively investigate the guanylin hormone-encoding gene (GUCA2A). Exploration encompassed expression patterns across tissues and single cells, clinical endpoints, methylation profiles, mutations, and immune and functional analyses. Subsequently, GUCA2Awas identified as a potential target for gain of function studies, leading to its amplification and cloning into gene constructs featuring both a robust CMV promoter and a cancer-specific MUC1 promoter. The succinylated PEI-9, characterized by low toxicity and high gene transfer efficiency, was then fabricated and characterized on HCT-116 cancer cells and normal Vero cell lines.
Results: systems biology studies revealed guanylin’saberrant expression patterns, methylation variations, and mutational changes as well as its remarkable association with immune engagement and poor survival outcomes in CRC. Moreover, SPEI-9 was introduced as a highly efficient and safe nanocarrier for gene delivery purposes. Additionally, in vitro studies revealed that both guanylin-expressing gene constructs exhibited the potential to inhibit cell growth and proliferation, inducing apoptosis, suppressing cell migration, and curtailing colony formation. Notably, these effects were more robust but non-specific in cancer cells treated with constructs containing the CMV general promoter, while, induction via the MUC1 promoter was more specific.
Conclusion: A genetic construct featuring the strong universal CMV and specific MUC1 promoter, expressing the guanylin peptide hormone, demonstrated highly effective and specific anticancer effects when transfected with nanocarriers characterized by high efficiency and low cytotoxicity. This nano-system holds promising implications for targeted CRC therapy.
Preparation of SnS 2 /MWCNTs/rGO chemically modified electrode and its electrochemical detection of H 2 O 2
Authors: Ye Zhidong , Tao Meixian , Song Xi , Duan Zhihong , Yao Chaoran , Shi Keyu , Jiang Qi 
Published: July 2024

Abstract

In view of the importance of rapid detection of H2O2, a carbon-based three-dimensional conductive network consisting of multiwalled carbon nanotubes (MWCNTs) and reduced graphene oxide (rGO) was introduced into the preparation of SnS2 to obtain SnS2/MWCNTs/rGO modified composite, which was prepared by one-step hydrothermal method. When the mass ratio of MWCNTs and rGO was 1:1, the composite had the best detection effect on H2O2. At this time, SnS2 was uniformly distributed on the surface of tubular MWCNTs and flaky rGO, and MWCNTs were interspersed between layers of rGO. The synergistic effect of the three materials showed a high current response ability for the detection of H2O2. During the range of 0.238 ~ 12.267 mmol·L− 1, the linear equation was Ipc(mA) = -0.029–0.019c(m mol·L− 1), with a sensitivity of 388.28 µA·mmol− 1·L·cm− 2 and a detection limit of 0.236 µmol·L− 1(S/N = 3). Moreover, the prepared modified electrode also had a good selectivity, reproducibility and stability.
An Innovative Process Suitable to Produce Back Contact for CdTe/CdS Thin- Film Solar Cells
Authors: Pal Jayanta , Patra Santanu Kumar 
Published: July 2024

Abstract

A novel process, different in all steps from the common process usually used for back contact to CdTe/CdS thin- film solar cells, is described here. This process on the one hand permits one to obtain high-efficient solar cells and on the other hand the stability of CdTe/CdS solar cells increases. The back contact is made with a thin intermediate layer of CuxO in between CdTe and contact material, which reduces the diffusion of Cu through the grain boundary of the CdTe to increase the stability of the cells. The charge polarization in CuxO may be a major cause in contact application of CdTe/metal structure.
Decomposition of Third-Order Symbolic Tensors for Outer Inverse Computation
Authors: Karmakar Biswarup , Panigrahy Krushnachandra 
Published: July 2024

Abstract

In symbolic computation, variables are stored as exact without numerical values to ensure precision throughout the calculations, which makes it an important area in computer algebra and scientific computing. A novel decomposition for third-order tensors within the t-product framework called tQDR decomposition is proposed to compute the outer inverse of symbolic tensors (tensors having polynomial entries and rational entries). In the process of introducing tQDR decomposition, a full rank factorization of tensors is also investigated. It is shown that many properties of full-rank decomposition of matrices (second-order tensors) do not hold in higher dimensions. Further, an algorithm inspired by the Leverrier-Faddeev algorithm is also introduced for efficiently computing the t-Moore–Penrose inverse of third-order symbolic tensors.
MSC Classification (2020): 15A09 , 15A69 , 68W30
Morphometric Traits as Efficient Indicators of Larval Stage-Specific Plasticity in Silver Pompano, Trachinotus blochii (Lacépède, 1801)
Authors: R Silpa , Landge Asha T. , S Surya , Ramteke Karankumar , Iburahim.S Abuthagir , Devadas Dayal 
Published: July 2024

Abstract

Morphological development, allometric growth, and behaviour of hatchery-reared silver pompano ,Trachinotus blochii were studied from hatching to metamorphosis (0 days post-hatch to 23 days post-hatch). In view of larval identification, to surpass critical periods in culture practices a total of 230 larval specimens of hatchery reared silver pompano were collected. A total of five post-embryonic stages were identified based on differences in morphology namely; Yolk sac (0–3 dph), Pre flexion (4–10 dph), Flexion (11 dph), Post flexion (12 dph), Juvenile (13–23 dph). The length has progressively increased in each larval stage, ultimately reaching 24 mm by the end of metamorphosis. This study showed that the development of the eye, head, and body depth had slower growth than the fish growth in length. All the morphometric traits efficiently differentiated each larval stage due to differences in stage-specific plasticity. This morphometric, morphological, and allometric study in the larval stage of Silver pompano will be a baseline data to strengthen the easy identification of larval stages, improve culture practices and supplement future studies on the species.
Xenogenous implanted dental follicle stem cells promote periodontal regeneration throughinduces N2 phenotype of neutrophils
Authors: LIU LI , Wen Yuqi , Chen Liangrui , Li Maoxue , Yu Jialu , Tian Weidong , Wu Yafei , Guo Shujuan 
Published: July 2024

Abstract

Background
Periodontal tissue loss is the main reason of tooth mobility and loss caused by periodontal disease. Dental follicle stem cells (DFSCs) have significant therapeutic potential in periodontal regeneration, which maybe mainly depends on its potent immunomodulatory capacity. Consequently, this study aims to elucidate the impact of implanted xenogenous DFSCs on innate immune responses during early and late stage in periodontal defect repair period.
Methods
To trace and investigate the immunomodulation mechanisms of DFSCs in vivo, DFSCs were engineered (E-DFSCs) using lentiviral vectors expressing CD63-enhanced green fluorescent protein (CD63-EGFP) and β-Actin-mCherry protein (ACTB-mCherry) to exhibit green and red fluorescence. The biological characteristics and functions of E-DFSCs were verified by proliferation, differentiation and co-culture experiments in vitro. In vivo, the periodontal regeneration capacity of E-DFSC was detected by implantation of murine periodontal defect model, and the response of innate immune cells were detected at 1st ,3rd, 5th day (early stage) and 4th week (late stage) after implantation.
Results
In vitro assessments showed that E-DFSCs retain similar properties to their non-engineered counterparts but exhibit enhanced macrophage immunomodulation capability. In mice models, four-week micro-CT and histological evaluations indicated that E-DFSCs have equivalent efficiency to DFSCs in periodontal defects regeneration. At the early stage of repair in mice periodontal defect, fluorescence tracking showed that implanted E-DFSCs might primarily activate endogenous cells through direct contact and indirect actions, and most of these cells is myeloperoxidase positive neutrophils. Additionally, compared with the control group, the neutrophils infiltration and conversion of N2-type were significantly increased in the E-DFSC group. At the late stage of defect regeneration, more M2-type macrophages, fewer TRAP + osteoclasts and upregulated OPG/RANKL ratio were detected in the E-DFSC group compared to the control group, which indicated that immune balance tilt toward healing and bone-formation.
Conclusion
The xenogenous implanted DFSCs can induce N2 phenotype of neutrophils in early stage, which can activate the innate immune mechanism of the host to promote periodontal tissue regeneration.