bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adipocyte reporter assays: Application for identification of anti‐inflammatory and antioxidant properties of mangosteen xanthones
Authors: Shen Qiwen , Chitchumroonchokchai Chureeporn , Thomas Jaisa L. , Gushchina Liubov V. , DiSilvestro David , Failla Mark L. , Ziouzenkova Ouliana 
Published: September 2013

ScopeThree fluorescence biosensors were developed based on a 3T3‐L1 preadipocyte line that stably expressed Nfkb‐RE/GFP, Fabp4‐P/CFP, and Nrf2‐P/YFP fluorescent reporters. We hypothesized that nutraceuticals’ inflammatory, adipogenic, and antioxidant status will be identified based on the change in fluorescence in reporter adipocytes. We validated these assays with activators of NFκB, FABP4‐regulating peroxisome proliferator activated receptor gamma, NFR2 and, thereafter, tested known and unknown properties of mangostines (MGs), the xanthone metabolites in mangosteen fruit.Methods and resultsWe validated inflammatory and adipogenic properties of α‐MG using an Nfkb‐RE/GFP biosensor assay. Next, we identified unique properties of γ‐MG, a minor MG xanthone. γ‐MG suppressed adipogenesis and expression of adiponectin, but inhibited the Nfkb‐RE/GFP reporter and secretion of inflammatory monocyte chemotactic protein 1 as compared to the control adipocytes. We found that the inhibition of adipogenesis and Nfkb‐mediated inflammation depends on a dose‐dependent reduction of Nrf2 promoter activity by α‐MG. The Nrf2 inhibition resulted in the reduced Pparg expression. α‐MG did not directly influence Pparg activity in Fabp4‐P/CFP adipocytes.Conclusionα‐MG‐mediated antioxidant response via Nrf2 is a mechanism preventing adipogenesis and inflammation in adipocytes. Combined application of high‐throughput biosensors could provide an effective platform for the identification of nutraceuticals and the mechanism of their actions in adipocytes and, potentially, in obese patients.
Differential hepatic GSTA2 expression of arylalkyl isothiocyanates in vivo and in vitro: The molecular mechanism of gene induction by phenethyl isothiocyanate
Authors: Gum Sang Il , Cho Min Kyung 
Published: September 2013

ScopeNaturally occurring isothiocyanates (ITCs) have been investigated for their chemopreventive actions. However, limited studies have determined the potential for regulation of hepatic glutathione S‐transferase A2 (GSTA2) by arylalkyl or alkyl ITCs.Methods and resultsThe present study was designed to investigate the effects of ITCs on GSTA2 expression and upstream signaling analyzed by Northern blotting, RT‐PCR, immunoblotting, and promoter‐luciferase assay. A single dose of ethyl isothiocyanate and phenethyl isothiocyanate (PEITC) enhanced GSTA2 mRNA levels in rats. The three consecutive injections of arylalkyl ITCs resulted in the gene induction. PEITC was the most potent and sustained inducer of GSTA2 in vivo. PEITC induced GSTA2 mRNA and protein expression in H4IIE cells. PEITC increased the levels of nuclear factor erythroid 2 related factor 2 and CCAAT/enhancer binding protein β (C/EBPβ). The cells transfected with a pGL‐ΔARE or pGL‐ΔC/EBP‐deleted promoter construct failed to increase GSTA2 promoter activity by PEITC. These effects of PEITC were downstream of multiple cellular signaling, including phosphoinositide 3‐kinase (PI3K), c‐jun N‐terminal kinase (JNK), and/or protein kinase A (PKA).ConclusionThese results demonstrated that PEITC is the most efficacious of the arylalkyl ITCs for the induction of GSTA2 in vivo and in vitro, and that transactivation of C/EBP and nuclear factor erythroid 2 related factor 2 downstream of multiple signaling pathways is involved in this induction.
Clinical characteristics and treatment of azoospermia and severe oligospermia patients with Y‐chromosome microdeletions
Authors: Zhang Fengbin , Li Lejun , Wang Liquan , Yang Liming , Liang Zhongyan , Li Jingping , Jin Fan , Tian Yonghong 
Published: September 2013

SUMMARYClinical characteristics, testicular pathology, serum levels of reproductive hormones, and genetic analysis were compared among 100 azoospermic, 20 oligozoospermic cases with azoospermia factor (AZF) microdeletion, and 50 fertile males to evaluate the relationship between the AZF microdeletion regions and the azoospermia phenotype. AZF microdeletion region, testicular volume, and serum reproductive hormone levels of patients were compared against histological examination of testicular biopsies. The number of cases of AZFa, AZFb, AZFc, AZFb + c, and AZFa + b + c microdeletion was respectively 2 (1.7%), 15 (12.5%), 77 (64.2%), 24 (20.0%), and 2 (1.7%). The testicular volume of patient with AZF microdeletion was smaller (P < 0.01), while luteinizing hormone (LH) and follicle‐stimulating hormone (FSH) levels were significantly higher than that of fertile individuals (P < 0.01 and P < 0.05, respectively). Among the patients, testicular volume with AZFb‐microdeletion cases was larger compared to patients with AZFc or AZFb + c microdeletions (P < 0.05 and P < 0.01, respectively), whereas FSH levels were significantly lower than that of AZFc or AZFb + c microdeletions (P < 0.05). The Johnsen score of patients with an AZFb + c microdeletion was lower than that of patients with AZFb and AZFc microdeletions, but no significant difference was observed. Pathological findings of testicular biopsies poorly correlated with the pattern of AZF deletion, with the AZFc microdeletion exhibiting the most varied phenotypes. In subsequent assisted reproductive treatments, sperm from patients with an AZFc microdeletion that was obtained by testicular sperm aspiration (TESA) or microdissection testicular sperm extraction (m‐TESE) were more likely to result in pregnancy. Combined with testis pathology pattern, the specific region of AZF microdeletion and hormonal assessments provide reliable prognostic information on the chance of successful sperm retrieval for assisted reproductive technologies. Mol. Reprod. Dev. 80: 908–915, 2013. © 2013 Wiley Periodicals, Inc.
The novel, actin‐like protein Tact3 is expressed in rodent testicular haploid germ cells
Authors: Oh Sung‐Dug , Park Soo‐Yun , Park Jae‐Il , Chun Sang‐Young , Ryu Tae‐Hun , Soh Jaemog 
Published: September 2013

SUMMARYMouse testis actin‐like proteins 1 and 2 (mTact1 and mTact2), which are expressed in murine haploid germ cells, have been described previously. Here, we report the cloning and characterization of a third actin‐like protein from rat, rat testis actin‐like protein 3 (rTact3). The complete cDNA of the rTact3 gene was approximately 3.7 kb in length, and its corresponding amino acid sequence consisted of 1219 amino acids. The rTact3 gene lacks introns, similar to mTact1 and mTact2. The 356 C‐terminal amino acids of rTact3 showed 43% homology with mTact1, whereas the 863 N‐terminal amino acids did not show any significant homology. Northern blot analysis revealed that rTact3 mRNA was expressed only in adult rat testes and not during the prepubescent stage. In situ hybridization revealed that rTact3 was expressed exclusively during round and elongated spermatids maturation stages in rat testes. Immunohistochemical experiments using antibodies raised against a synthetic peptide showed that the expression of the rTact3 protein was also restricted in round and elongated spermatids, specifically in the head and acrosome of mature rat sperm. The 5′‐flanking region of the mTact3 gene was found to contain a TATA‐box motif as well as two putative CREB/c‐Jun and five C/EBP motifs. mTact3 promoter activity was enhanced in a dose‐dependent manner by the transfection of CREB, c‐Jun, or C/EBP in NIH3T3 cells. These results suggest that Tact3 proteins might play an important role in rodent germ‐cell development. Mol. Reprod. Dev. 80: 988–999, 2013. © 2013 Wiley Periodicals, Inc.
Risk factors associated with increased depressive symptoms among Latinas diagnosed with breast cancer within 5 years of survivorship
Authors: Aguado Loi Claudia X. , Baldwin Julie A. , McDermott Robert J. , McMillan Susan , Martinez Tyson Dinorah , Yampolskaya Svetlana , VandeWeerd Carla 
Published: September 2013

AbstractBackgroundCo‐occurring depression in women with cancer can complicate cancer treatment, lead to poor treatment adherence for both conditions, and decrease survival if left untreated. The purpose of this study was to explore risk factors for depression among Latina breast cancer survivors.MethodsA closed‐ended questionnaire was administered by telephone to 68 Latinas diagnosed with breast cancer within the past 5 years. Depression symptoms were measured using the Patient Health Questionaire‐9 with the Endicott substitutive criteria applied. The Cognitive Appraisal Health Scale and the Brief COPE were used to measure appraisal and coping. Descriptive statistics, bivariate and multiple linear regression analyses were completed.ResultsApproximately 45.6% of women reported depressive symptoms. Multivariate analyses showed that a cognitive appraisal variable (decreased challenge or the potential to overcome), coping variables (increased acceptance, less positive reframing, less active coping, less use of emotional support, substance use and more self‐blame), poor body image, less family and peer support to be significantly associated with an increased risk for depression. Intrapersonal variables accounted for the greatest explained variance (69%).ConclusionsThis study identified several risk factors for depression. Study findings highlight the need for intervention programs to help women normalize emotions and thoughts related to cancer and its treatments, and to improve their cognitive abilities to overcome, accept, and positively reframe cancer and other difficult situations women face throughout the cancer continuum. The importance of family and peer support to improve depressive symptoms was also evident. Copyright © 2013 John Wiley & Sons, Ltd.
Job attributes, job satisfaction and the return to health after breast cancer diagnosis and treatment
Authors: Barnes Andrew J. , Robert Nicholas , Bradley Cathy J. 
Published: September 2013

AbstractBackgroundAs detection and treatment of cancer has advanced, the number of working age women with breast cancer has increased. This study provides new information on the intersection of breast cancer treatment and job tasks and how, together, they impact employed and newly diagnosed women.MethodsThe sample comprised 493 employed women within 2 months of initiating treatment. Job satisfaction and demands were assessed by a pre‐diagnosis recall along with measures of mental and physical health and assessed again 9 months after initiating treatment. Using seemingly unrelated regression, we tested the effect of job tasks and satisfaction on mental and physical health 9 months post‐treatment initiation, controlling for pre‐diagnosis health status, patient characteristics, and job tasks.ResultsPhysical job demands prior to diagnosis were not significantly associated with mental or physical health 9 months after treatment initiation. Employment in cognitively demanding and less satisfying jobs was associated with decreases in mental health and increases in problems with work or daily activities 9 months post‐treatment initiation (p<0.05). Women who received five or more cycles of chemotherapy reported lower vitality, social functioning, and worse measures of physical health compared with those who did not receive chemotherapy (p<0.05).ConclusionsEmployment in cognitively demanding and unsatisfying jobs may impede mental health recovery, particularly in patients who receive longer chemotherapy regimens. Such information may be used by patients and clinicians in deciding when to undergo chemotherapy and whether job tasks can be restructured to hasten recovery. Copyright © 2013 John Wiley & Sons, Ltd.
Zur Ermüdungsbewertung der Böenantwort von Türmen
Authors: Kemper Frank H. , Feldmann Markus 
Published: September 2013

AbstractWindbelastete Türme aus Stahl weisen vielfach dynamische Beanspruchungswechsel in einer Größenordnung auf, die aus wirtschaftlichen Erwägungen nicht mehr mit quasistatischen Modellen abgebildet werden können, da damit die Materialausnutzung im Grenzzustand der Ermüdung auf das Dauerfestigkeitsniveau beschränkt ist. Im vorliegenden Beitrag wird ein modernes Berechnungsverfahren präsentiert, das die Beanspruchbarkeit im Grenzzustand der Ermüdung auf Betriebsfestigkeitsebene mittels stochastischer Methoden berechenbar macht. Aufgrund der analytischen Formulierung des Berechnungsweges lassen sich auch instationäre Last‐Admittanzen, wie sie beispielsweise bei Windenergieanlagen auftreten, mit großem Zeitvorteil gegenüber transienten Verfahren berücksichtigen. Neben einem baupraktischen Anwendungskonzept für die Ermüdungsbewertung einfacher Strukturen werden die Vorzüge der stochastischen Modellbildung auch exemplarisch anhand einer Windenergieanlage aufgezeigt. Die Anwendungsbeispiele demonstrieren die Eignung des Verfahrens für baupraktische Zwecke.Verification of the fatigue limit state of gust induced structural response of tower constructions. Tower structures made of steel, show often stress cycle amplitudes due to the wind loading, which cannot be treated by quasi‐static models anymore for economic reasons. In the present paper a methodology is described which allows for a detailed verification of the fatigue limit state, using a realistic stress cycle count distribution. Due to the analytical formulation of the approach, it is possible to consider nonsteady load admittance relations, which are present in e. g. in case of wind turbines or structures with cylindrical cross‐sections with a low computational effort, compared to transient methods. Besides a practical concept for the verification of simple structures, the advantages of the method are shown exemplary using the example of a wind turbine with lattice tower. The shown examples emphasize the usability for structural problems.
Nachhaltigkeitsbewertung stählerner Tragkonstruktionen von Windenergieanlagen
Authors: Schaumann Peter , Bechtel Anne 
Published: September 2013

AbstractDie Berücksichtigung eines ganzheitlichen Dimensionierungskonzepts, das neben den bisher maßgebenden Konstruktionsfaktoren Tragfähigkeit und Wirtschaftlichkeit weitreichendere Nachhaltigkeitseffekte wie beispielsweise ökologische Auswirkungen integriert, nimmt zukünftig einen wachsenden Stellenwert bei der Wahl von Tragstrukturen ein. Auf Grundlage bereits bekannter Nachhaltigkeitsbewertungssysteme aus dem Hochbau wurde im Rahmen des Forschungsprojektes NaStafEE eine Methode zur Nachhaltigkeitsbewertung stählerner Tragkonstruktionen für regenerative Energien entwickelt. Es wurden für die Kategorien Ökologie, Ökonomie, Soziales, Technik und Prozess Kriterien definiert, die die Nachhaltigkeit der stählernen Tragstrukturen über den gesamten Lebenszyklus erfassen und somit eine Bewertung der Stahltragstruktur von Offshore‐Windenergieanlagen ermöglichen. Die Bewertungsmethode sowie Untersuchungsergebnisse für zwei Offshore‐Stahlstrukturen werden in diesem Beitrag vorgestellt.Sustainability assessment of steel constructions of offshore wind energy turbines. The consideration of an holistic design approach, taking into account not only the load bearing capacity and economical effects but also sustainability effects as e.g. ecological aspects, will have an increasing impact to the construction choice in future. Based on already known sustainability assessment methods originating from the building sector a sustainability assessment applicable for steel structures of renewables was developed within the research project NaStafEE. Criteria for the sustainability categories ecology, economy, social responsibility, engineering and process were generated to enable the assessment of the sustainability of steel constructions of offshore wind energy turbines. The assessment method as well as sustainability results for two offshore structures we presented within this paper.
Tragverhalten randnaher Betondübel unter Längsschubbeanspruchung
Authors: Gajda Joanna , Kurz Wolfgang 
Published: September 2013

AbstractVerbundkonstruktionen aus Stahl und Beton haben sich in den letzten Jahrzehnten zu einer wirtschaftlichen und konkurrenzfähigen Bauweise im Hoch‐ und Brückenbau entwickelt. An die innovativen Verbundmittel wie Betondübel werden aufgrund der neuen Entwicklungen der Konstruktionen hohe Anforderungen hinsichtlich der Tragfähigkeit und der Verformbarkeit gestellt. Die Verbundmittel werden in immer schlankeren Konstruktionen eingesetzt. Die Betondübel in der Nähe des Plattenrandes können, ähnlich wie die liegenden Kopfbolzendübel, bei Einleitung der Längsschubkräfte versagensrelevante Spaltzugspannungen in Plattendickenrichtung erzeugen. Dieser Versagensmechanismus für Betondübel mit geringer Betondeckung senkrecht zur Verbundmittelachse wurde bislang in den Bemessungsmodellen nicht explizit berücksichtigt. Erste Versuche zur Bestimmung der Trag‐ und Verformungsfähigkeit randnaher Betondübel in Abscher‐ und Ausziehversuchen wurden durchgeführt. Relevante Parameter, die Einfluss auf das Trag‐ und Verformungsverhalten bei randnahen Betondübeln haben, werden anhand der Versuche diskutiert.Load bearing behavior of concrete dowels in a location close to a free surface under longitudinal shear stress. Over the last decades, composite structures made of steel and concrete have been developed in order to allow for the economic and competitive construction of buildings and bridges. Due to the new developments of innovative shear connectors such as concrete dowels, high requirements are expected for the load‐bearing capacity and the ductility. The shear connectors are often used in slender structures. Concrete dowel close to a free concrete surface of a slab introduce longitudinal shear forces, which cause failure‐ relevant splitting stresses in direction of the slab thickness, similar to the horizontal lying headed studs. This failure mechanism of concrete dowels with a low concrete cover perpendicular to the central axis of the shear connectors has not been explicitly considered in the structural design models. First experiments in order to investigate the load‐bearing capacity and the ductility of concrete dowels in a location close to a free surface in shear and pull‐out tests were carried out. Some relevant parameters that have influence on the load‐deformation behavior of concrete dowels located closely to the surface are discussed with reference to the experiments.
Zum Einfluss der Härte von Brennschnittkanten auf die Haltbarkeit von Beschichtungen
Authors: Jost Katrin , Renner Anja , Lange Jörg 
Published: September 2013

AbstractDie mittlerweile baurechtlich eingeführte DIN EN 1090‐2, die die Ausführung von Stahltragwerken regelt, gibt für Schnittflächen von Stahlblechen einen zulässigen höchsten Härtewert an. Solche Grenzwerte waren in den zuvor geltenden deutschen Normen nur aus der Schweißverfahrensprüfung bekannt. Da die gemessene Härte den neuen Grenzwert bei Schnittkanten, die mit den gängigen Schnittverfahren hergestellt wurden, oft überschreitet, stellt die neue Regelung die produzierenden Firmen vor große Probleme. Insbesondere bei planmäßig nicht weiterbearbeiteten Schnittkanten stellt sich die Frage, inwieweit die Aufhärtungen die Oberflächenvorbereitung für den Korrosionsschutz beeinträchtigen und sich negativ auf die Haftzugfestigkeit der Beschichtung auswirken. Um herauszufinden, welche Zusammenhänge hier bestehen, wurden an verschiedenen Brennschnittkanten Versuche zur Bestimmung der Härte und der Rauigkeit durchgeführt und der Einfluss der Kantenhärte auf die Haltbarkeit von Beschichtungen untersucht.The influence of the hardness of heat cut edges. The meanwhile introduced DIN EN 1090‐2, which settles the execution of steel structures, gives a maximum permissible hardness value for cut edges of steel plates. In German standards comparable limit values where thitherto only known in conjunction with the welding procedure qualification. Due to the fact that the measured hardness on edges, which were cut with the established cutting methods, often exceeds this new maximum value, producing firms are faced with problems. Especially for edges that do not get milled the question arises, what influence the increase of hardness has on the surface preparation for anticorrosive coating and whether it has any effect on the adhesive tensile strength of the coating. To find out which correlations exist here, tests were conducted on different thermal cut edges. Hardness and roughness were measured and the influence of these values on the resistance of the coating was tested.
Sphäre Deutsche Bank, Frankfurt am Main
Authors: Castegini Giulio , Fahlbusch Mark , Tessmann Oliver , Drawer Olive , Arnold Uwe 
Published: September 2013

AbstractIm Jahre 2006 hat Mario Bellini den Architekturwettbewerb für die Sanierung der Firmenzentrale der Deutschen Bank AG in Frankfurt am Main gewonnen. Unter seiner Leitung ist in der Eingangshalle eine herausragende Skulptur entstanden. Die Sphäre – der architektonische Mittelpunkt des neu gestalteten Foyers zwischen den beiden Türmen, symbolisiert Stabilität und Stärke, aber zugleich auch Dynamik und Wandel.Der Entwurf des Architekten sah eine 16 m durchmessende Kugel vor, die mit Bändern umwickelt zu sein scheint und zwischen den beiden Türmen der Bank klemmt. Dieses Konzept wurde durch ein interdisziplinäres Team bestehend aus dem verantwortlichen Entwurfsarchitekt Giulio Castegini (Mario Bellini Architects), den Ingenieuren Oliver Tessmann und Mark Fahlbusch (Bollinger Grohmann Ingenieure) und Oliver Drawer der ausführenden Firma (Arnold AG) weiterentwickelt. Das Projektmanagement verantwortete Drees
Modernes Eisentragwerk über klassizistischem Rundbau
Authors: Möller Eberhard 
Published: September 2013

AbstractDie Befreiungshalle bei Kelheim nahe Regensburg zählt zu den bedeutendsten politischen Monumentalbauten des 19. Jahrhunderts. Vor 150 Jahren, am 18. Oktober 1863, findet die Einweihung statt. Es ist zugleich der 50. Jahrestag der Völkerschlacht bei Leipzig. Der bayerische König Ludwig I. hatte den Bau 1836 bei seinem favorisierten Architekten Friedrich von Gärtner (1791–1847) in Auftrag gegeben, zum einen, um an den Sieg über Napoleon zu erinnern, aber auch, um einer “größeren deutschen Nation“ ein Denkmal zu errichten, um einen “Pantheon der Teutschen“ zu bauen (Bild 1). Im Archäologischen Museum der Stadt Kelheim präsentiert das Architekturmuseum der TU München bis zum 27. Oktober 2013 alle wichtigen originalen Zeichnungen und Pläne aus der Bauzeit (Bild 2).The Hall of Liberation near Kelheim – Modern iron architecture over classicist rotunda. The Befreiungshalle (Hall of Liberation) near Kelheim in Bavaria is one of the most important 19th century monuments. 150 years ago, on 18 October 1863, the inauguration takes place. It is the 50th Anniversary of the Battle of Leipzig. The Bavarian King Ludwig I had ordered the construction in 1836 at his favorite architect Friedrich von Gärtner (1791–1847) in order to commemorate the victory over Napoleon, but also to build a monument towards a “larger German nation”, a “Pantheon of the Germans”. At the Archäologisches Museum Kelheim an exhibition shows all important original sketches, coloured drawings and plans till 27 October 2013.