bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insulin receptors in vascular smooth muscle cells regulate plaque stability of atherosclerosis
Authors: Li Qian , Fu Jialin , Park Kyoungmin , Shah Hetal , Li Qin , Wu I Hsien , King George L 
Published: August 2024

Abstract

Aims
Increased prevalence of acute myocardial infarction related to diabetes and insulin resistance is associated with an elevated risk of unstable atherosclerotic plaques, which are characterized by reduced vascular smooth muscle cells (VSMCs) and extracellular matrix (ECM) and increased inflammation. Thus, insulin resistance may reduce plaque stability, as deleting insulin receptors (IRs) in VSMCs decreases their proliferation and enhances apoptosis.

Methods and results
Direct effects of insulin on VSMCs to alter plaque composition were studied using mice with double knockout of ApoE and IR genes in VSMCs with SMIRKO/ApoE−/−, Myh11-CreERT2EYFP+/ApoE−/−, and Myh11-CreERT2EYFP+IRKO/ApoE−/− mice, which were also used for lineage tracing studies. Compared with ApoE−/− mice, SMIRKO/ApoE−/− mice exhibited more atherosclerotic plaques, which contained less VSMCs and collagen but increased levels of VSMC apoptosis and necrotic areas. Lineage tracing studies showed that Icam1+ Vcam1+ VSMC was inflammatory, which increased in the aortas of Myh11-CreERT2EYFP+IRKO/ApoE−/− mice compared with control mice. Isolated VSMCs lacking IRs expressed higher inflammatory cytokines than cells with IRs. Cell-based studies indicated that insulin’s anti-apoptotic and pro-proliferative effects in VSMCs were mediated via activation of the IR/Akt pathway, which were decreased in VSMCs from SMIRKO or high-fat diet mice. An analysis of the IR targets that regulated inflammatory cytokines in VSMCs showed that thrombospondin 1 (Thbs1) and Mmp2 were consistently increased with a loss of IRs. Insulin inhibited Thbs1 expression, but not Mmp2 expression, through p-Akt/p-FoxO1 pathways in VSMCs from ApoE−/− mice, and was impaired in cells from SMIRKO/ApoE−/− mice. Thbs1 further induced Icam1 and Mmp2 expressions in VSMCs.

Conclusion
Insulin via IRs has significant actions in VSMCs to decrease inflammation, apoptosis, and ECM turnover via the activation of Akt and FoxO1 pathways. The inhibition of insulin actions and related pathways related to insulin resistance and diabetes may contribute to the formation of unstable atherosclerotic plaques.
School composition and academic decisions
Authors: Rosenqvist Erik , Brandén Maria 
Published: August 2024

Abstract
Research on the effects of school composition tends to focus on how it shapes school achievement. In this study, we instead examine how school composition shapes children’s educational aspirations, given their achievement, and if children from different socio-economic backgrounds are affected differently. We apply school-fixed effects on Swedish register data, including all 9th-grade students from 2013 to 2017. Being exposed to a high share of low-achieving schoolmates increases the likelihood of applying for academics instead of vocational tracking across socio-economic backgrounds. In contrast, the share of high-achieving schoolmates is negatively associated with academic tracking only for high-SES children. Being exposed to peers with highly educated parents increases the likelihood of applying for academic tracking for low-SES children, whereas the effect is weaker or even negative for some of the high-SES groups. Together, our results suggest that the academic decisions of both high- and low-SES children could benefit from a less segregated school environment.
Go west, young bunting: recent climate change drives rapid movement of a Great Plains hybrid zone
Authors: Dougherty Paul J , Carling Matthew D 
Published: August 2024

Abstract
Describing how hybrid zones respond to anthropogenic influence can illuminate how the environment regulates both species distributions and reproductive isolation between species. In this study, we analyzed specimens collected from the Passerina cyanea×P. amoena hybrid zone between 2004 and 2007 and between 2019 and 2021 to explore changes in genetic structure over time. This comparison follows a previous study that identified a significant westward shift of the Passerina hybrid zone during the latter half of the twentieth century. A second temporal comparison of hybrid zone genetic structure presents unique potential to describe finer-scale dynamics and to identify potential mechanisms of observed changes more accurately. After concluding that the westward movement of the Passerina hybrid zone has accelerated in recent decades, we investigated potential drivers of this trend by modeling the influence of bioclimatic and landcover variables on genetic structure. We also incorporated eBird data to determine how the distributions of P. cyanea and P. amoena have responded to recent climate and landcover changes. We found that the distribution of P. cyanea in the northern Great Plains has shifted west to track a moving climatic niche, supporting anthropogenic climate change as a key mediator of introgression in this system.
Looking Back on Career, Looking Forward to Retirement: Antecedents of Subjective Career Evaluations and Their Impact on Retirement Adjustment
Authors: Tunney Orlaith , Henkens Kène , van Solinge Hanna 
Published: August 2024

Abstract

Objectives
An individual’s past, and reflections on it, may influence current and future well-being. Recent qualitative studies suggest retirees’ recollections about their careers relate to well-being in retirement. We investigated associations between life course events and subjective career evaluations, gender differences in these associations, and their subsequent association with retirement adjustment.

Methods
We used data from 3 waves (2015, 2018, and 2023) of the NIDI Pension Panel Study (NPPS), a longitudinal survey of Dutch older workers. Using a sample of 6,109 respondents, we used ordinary least squares (OLS) regression models to investigate associations between employment stability facilitators and inhibitors (e.g., promotion, demotion, unemployment) and personal shocks (e.g., divorce, widowhood) and subjective evaluations of satisfaction with the work and family domains of career between genders. Using a follow-up sample (N = 4,106), we employed ordinal logistic regression models to investigate the impact of these baseline subjective evaluations on retirement adjustment at follow-up.

Results
Employment stability factors such as demotion and unemployment, and personal shocks such as poor psychological health were associated with subjective evaluations of the work and family career domains. Gender differences in these associations were found. Evaluations in both the work and family domains were associated with retirement adjustment at follow-up.

Discussion
Our results demonstrate the importance of life course events on older workers’ evaluations of their careers and the long-term impact of subjective career evaluations. Further research is needed to evaluate the predictive utility of these evaluations for other outcomes in older adulthood.
The crustal and upper-mantle structure beneath NW Iran: an integrated analysis of surface waves and gravity data
Authors: Shirzad Taghi , VahidRavesh Shaghayegh , Mortezanejad Gholamreza , Abdollahi Somayeh , Kakhki Mohsen Kazemnia , Naghavi Mojtaba , Rahimi Habib , Hatami Mohammad Reza 
Published: August 2024

SUMMARY
Understanding the crustal seismic characteristics of tectonically active regions is crucial for seismic hazard assessment. The study conducted in NW Iran utilized surface wave tomography, radial anisotropy and density information to analyse the complex crustal structure of the region, which is outstanding because of diverse tectonic features, sedimentary basins and volcanic formations. By selecting a data set of 1243 events out of over 3500 earthquakes with M &gt; 4, and employing strict data selection criteria (such as SNR, M and Δ), the researchers calculated Rayleigh and Love wave group velocity dispersion curves using Gaussian multiple filters and phase-matched filtering. The tomographic procedure was initiated by excluding data with residuals &gt; 2σ for enhanced stability. Individual inversions were then carried out for local Rayleigh and Love wave dispersion measurements to obtain 1-D VSV and VSH models. Radial anisotropy and VSiso were determined through a discrepancy and averaging of the obtained VSH and VSV, respectively. Gravity modelling was also employed alongside surface wave analysis to understand the region's complex geology, revealing insights into upper-middle-lower crust boundaries, subsurface structures and Moho depths. The study's velocity maps reveal significant findings related to geological units and tectonic features in various regions based on the provided results. Low velocities in the South Caspian Basin (SCB) and Kura Depression (KD) regions are attributed to substantial sedimentary layers, while low velocities, and depth of VS in NW Iran and Eastern Anatolian Accretionary Complex (EAAC) regions suggest the presence of partially molten materials in the upper and middle crust. The Sanandaj–Sirjan Zone (SSZ) region shows a low-velocity anomaly in longer periods and greater depths of VS, surrounded by normal to high velocities, indicating a thick middle crust. Analysing radial anisotropy and VSiso profiles offers insights into upper-middle-lower crust boundaries, subsurface structures and Moho depths, highlighting middle crust thickening and lower crust thinning beneath the SSZ. The study confirms the gentle subduction of the SCB oceanic-like lower crust beneath NW Iran in the Talesh (TAL) region, with a rigid middle crust. Additionally, cross-sections reveal igneous laccoliths underplate with a VSiso of 3.7 km s−1 in the volcanic region. The difference observed by subtracting the velocity models at two adjacent depths, combined with parametric test results, indicates that the Sahand volcanic system is clearly identifiable, while the influence of subtle subduction on the Sabalan volcano at depths up to 30 km remains less distinct. The magma chamber beneath Sahand is situated at depths ranging from 18 to 25 km.
Tackling Labour Monopsony by Gig Platforms: The Anti-Monopoly Law as an Alternative Legal Approach to the Labour Law System in China
Authors: Zhao Zeyu , Luo Huanxin 
Published: August 2024

Abstract
In the gig economy, workers could be subject to exploitative and coercive practices by platforms and their stringent labour algorithms. Since gig workers are not defined as typical employees, the Chinese labour law system fails to adequately protect gig workers. Furthermore, the limited scope of labour law protection is also another reason for the ineffectiveness of the Chinese labour law system. Even if gig workers can be identified as employees, the regulation of labour algorithms is uncovered by the existing labour laws. Additionally, collective bargaining rights of gig workers may help but as explained in Section 4, they are still immature and collective barging practices are likely to violate the Chinese Anti-Monopoly Law. This article claims that the enforcement of the Anti-Monopoly Law can be an alternative approach to protecting gig workers as the gig platforms may constitute a labour monopsony. Therefore, anti-trust enforcement can step in and punish exploitative and coercive practices harming gig workers. However, there are limitations of the Anti-Monopoly Law in dealing with these practices and thereby the advantages of the Chinese labour law system regarding the protection of gig workers should still be taken seriously.
CDC-Funded HIV Testing Services Outcomes in Ending the HIV Epidemic in the U.S. (EHE) and Non-EHE Jurisdictions, 2021
Authors: Patel Deesha , Mulatu Mesfin S , Wang Guoshen , May Alicia C , Moore Andrea , Rao Shubha 
Published: August 2024

Abstract

Background
“Ending the HIV Epidemic in the US” (EHE) focuses on 57 jurisdictions most affected by HIV.

Methods
Using 2021 data from the National HIV Prevention Program Monitoring and Evaluation system, we calculated distributions of Centers for Disease Control and Prevention–funded HIV tests and HIV testing services outcomes in EHE and non-EHE jurisdictions. We conducted χ2 tests and robust Poisson regression to compare differences in outcomes.

Results
Of the 1 753 873 tests, a greater proportion was conducted in EHE (65.7%) vs non-EHE (34.3%) jurisdictions (P &lt; .001). A greater number of persons newly diagnosed were in EHE (n = 5861) vs non-EHE (n = 2329) jurisdictions; newly diagnosed positivity was also higher in EHE (0.5%) vs non-EHE (0.4%) jurisdictions (prevalence ratio [PR], 1.31; 95% CI, 1.25–1.38). Among persons newly diagnosed, there were no differences in linkage to care within 30 days in EHE (77.9%) vs non-EHE (77.1%) jurisdictions (PR, 1.01; 95% CI, .95–1.07). The proportion of persons newly diagnosed interviewed for partner services was lower in EHE (69.0%) vs non-EHE (84.8%) jurisdictions (PR, 0.81; 95% CI, .76–.87).

Conclusions
Centers for Disease Control and Prevention–funded HIV testing services in EHE jurisdictions conducted more HIV testing and diagnosed more persons. Jurisdictions can further expand HIV testing and related services to continue striving toward EHE goals.
Effects of added 25(OH)D3 with varying standardized total tract digestible phosphorus concentrations on nursery pig performance, bone characteristics, and serum vitamin D status
Authors: Becker Larissa L , Gebhardt Jordan T , Tokach Mike D , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Bergstrom Jon R , Siepker Christopher L 
Published: August 2024

Abstract
A total of 360 pigs (DNA 600 × 241; initially 5.8 kg) were used in a 45-d growth study to evaluate the effects of adding 25(OH)D3 with 3 levels of standardized total tract digestible (STTD) P on nursery pig growth performance, bone and urine characteristics, and serum vitamin D. Pigs were weaned at 19 d of age and randomly allotted to 1 of 6 dietary treatments with 5 pigs per pen and 12 replications per treatment. Dietary treatments were arranged in a 2 × 3 factorial with main effects of 25(OH)D3 (0 or 50 µg/kg equivalent to 2,000 IU/kg of vitamin D3; Hy-D, dsm-firmenich, Plainsboro, NJ) and STTD P (70%, 100%, or 130% of the NRC [NRC 2012. Nutrient requirements of swine. 11th rev. ed. Natl. Acad. Press, Washington, DC) requirement estimate on a dietary percentage basis]. All diets contained 1,653 IU/kg of vitamin D3. On day 45, 1 pig per pen was euthanized to collect the right fibula, metacarpal, and 2nd and 10th ribs. Overall, increasing STTD P increased (quadratic, P ≤ 0.003) ADG, ADFI, and G:F with minimal improvement above 100% of the NRC STTD P requirement estimate. Added 25(OH)D3 had no effect on growth performance. Increasing STTD P decreased urinary Ca concentration (linear, P &lt; 0.001) and increased urinary P concentration (quadratic, P &lt; 0.001). When pigs were fed added 25(OH)D3, serum 25(OH)D3 increased (quadratic, P = 0.005) as STTD P increased but no differences were observed when 25(OH)D3 was not added and STTD P increased (25(OH)D3 × STTD P interaction, P = 0.032). When pigs were fed 25(OH)D3, serum 1,25(OH)2D3 increased (quadratic, P &lt; 0.001) as STTD P decreased but the increase was not significant when no 25(OH)D3 was fed (STTD P × 25(OH)D3 interaction, P = 0.002). Bone ash percentage and weight increased (quadratic, P ≤ 0.065) in all bones as STTD P increased. Added 25(OH)D3 had no effect on bone density or bone ash weight; however, the reduction in bone ash percentage observed with reducing STTD P level tended to be less when 25(OH)D3 was provided (linear interaction, P = 0.098). Increasing STTD P decreased the likelihood of abnormal histologic bone lesions in the 10th rib. In summary, added 25(OH)D3 had limited effect on growth performance; however, an increase in serum concentrations of 25(OH)D3 and 24,25(OH)2D3 was observed. The addition of 25(OH)D3 to P-deficient diets increased percentage bone ash. Increasing STTD P to 100% of NRC [NRC 2012. Nutrient requirements of swine. 11th rev. ed. Natl. Acad. Press, Washington, DC] requirement estimate increased growth and 130% of NRC maximized bone ash.
Phylogeny, biogeography, and host range of gall midges (Diptera: Cecidomyiidae) feeding on spores of rust fungi (Basidiomycota: Pucciniales)
Authors: Gómez-Zapata Paula Andrea , Johnson Melissa A , Bonacci Teresa , Aime M Catherine 
Published: August 2024

Abstract
Rust fungi (Pucciniales) are plant pathogens that can cause devastating yield losses to economically important crops and threaten native plants with extinction. Rusts are usually controlled with fungicides when rust-resistant plant varieties are unavailable. However, natural enemies may offer an alternative to chemicals by acting as biological controls. The larvae of Mycodiplosis Rübsaamen (49 spp.) feed on the spores of rusts and powdery mildew fungi and have been suggested as a potential biocontrol candidate for disease-causing rusts. However, little is known about the phylogenetic relationships, biogeography, and host range of this genus. We screened 5,665 rust specimens from fungarium specimens and field collections and recovered a total of 363 larvae on 315 rust specimens from 17 countries. Three mitochondrial and 2 nuclear loci were amplified and sequenced for the phylogenetic reconstruction of 129 individuals. We recovered 12 clades, of which 12 and 10 were supported with maximum likelihood and Bayesian inference, respectively. Of the 12 clades, 7 comprised species from multiple continents and climatic regions, and 5 comprised species from a single region. Individuals forming clades were collected from 2 to 18 rust species, suggesting that Mycodiplosis species have a broad host range. In total, Mycodiplosis larvae were identified on 44 different rust species collected from 18 plant families. Future studies should focus on expanding field sampling efforts, including data from additional gene regions, and incorporating morphological data to further elucidate species diversity and distribution patterns.
Opioid prescription status around surgery, bone metastasis, or death events among patients with breast cancer in Japan: an analysis of the Japanese public health insurance comprehensive claims database (the National Database)
Authors: Yoshida Manami , Miyashita Mitsunori , Saeki Toshiaki , Hiroi Shinzo , Morioka Yasuhide , Iwasaki Kosuke , Shimizu Eiko 
Published: August 2024

Abstract

Objective
To investigate the opioid prescription status around clinical events among patients with breast cancer in Japan using a comprehensive claims database.

Methods
This was a retrospective cohort study using the National Database (April 2009–March 2020). The target patients had a first breast cancer diagnosis in April 2010 or later. The percentages of patients prescribed opioids before and after surgery, before and after bone metastasis, and before death with a breast cancer diagnosis in the same month were analyzed by month and by clinical facility characteristics and location.

Results
We identified 1 085 388 target patients, including 216 503, 72 645, and 70 832 patients with data for the events of surgery, bone metastasis, and death, respectively. The percentage of patients prescribed opioids in the month of surgery was the highest of the entire study period at ≥70%. The percentage of patients prescribed opioids increased before bone metastasis, peaked 1 month later, and decreased thereafter while remaining higher than that before the event. The percentage of patients who were prescribed opioids before death increased over time, peaking at 33.4% 1 month before death. Prescriptions differed by facility characteristics and facility location around surgery; no differences by facility characteristics, including location, were noted around the other events. The percentage of patients prescribed opioids was consistently lower than that reported in other countries for all events.

Conclusions
We showed the opioid prescription status around clinical events, including some distinct patterns depending on facility characteristics for the period around surgery, among patients with breast cancer in Japan.
Responses of juvenile blacklegged ticks (Acari: Ixodidae) to hosts of varying quality
Authors: Burton Emily S , Ostfeld Richard S , Brunner Jesse L 
Published: August 2024

Abstract
Blacklegged ticks (Ixodes scapularis) are the most medically and economically important vectors in North America. Each of their 3 life stages requires a blood meal from one of many potential host species, during which they can acquire or transmit pathogens. Host species, however, vary tremendously in their quality for ticks, as measured by differences in feeding and molting success. There should be clear fitness benefits for ticks that preferentially feed upon high-quality hosts (e.g., white-footed mice, Peromyscus leucopus), or at least avoid feeding on very low-quality hosts (e.g., Virginia opossums, Didelphis virginiana). Indeed, laboratory experiments have found some evidence of host preferences in I. scapularis; but these involve presenting ticks with hosts simultaneously and measuring movement towards hosts on a horizontal plane. In nature, however, host-seeking ticks encounter hosts sequentially and their movements are principally in a vertical plane. Here, we present the results of a study in which we measured the vertical movements of host-seeking juvenile blacklegged ticks before and after a host (P. leucopus, Tamias striatus, Sciurus carolinensis, or D. virginiana) was present, and whether the strength of their responses varies with host quality. We found ticks did not measurably alter the speed of their vertical movement in the presence of any hosts, regardless of host quality. Both larvae and nymphs quested slightly higher in the presence of hosts, but this did not vary by host species. These results call into question the existence of active host preferences, at least in this stage of the host-seeking process.
Anatomy of recalcitrance: integrated imaging and spectroscopy reveal features of hard-to-root rose cuttings
Authors: Vilasboa Johnatan 
Published: August 2024

This article comments on:
Wamhoff D, Gundel A, Wagner S, Ortleb S, Borisjuk L, Winkelmann T. 2024. Anatomical limitations in adventitious root formation revealed by magnetic resonance imaging, infrared spectroscopy, and histology of rose genotypes with contrasting rooting phenotypes. Journal of Experimental Botany 75, 4784–4801. https://doi.org/10.1093/jxb/erae158
Unlocking fertility in the female gametophyte: a DEAD-box RNA helicase is essential for embryo sac development and seed setting
Authors: Ali Asif , Riaz Asad , Wu Xianjun 
Published: August 2024

This article comments on:
Huang J, Qiao Z, Yu H, Lu Z, Chen W, Lu J, Wu J, Bao Y, Shahid MQ, Liu X. 2024. OsRH52A, a DEAD-box protein, regulates functional megaspore specification and is required for embryo sac development in rice. Journal of Experimental Botany 75, 4802–4821. https://doi.org/10.1093/jxb/erae180
RAM1 orchestrates arbuscular mycorrhizal symbiosis in non-legumes
Authors: Gautam Chandan Kumar , Mutyala Prema , Das Debatosh 
Published: August 2024

This article comments on:
Ho-Plágaro T, Tamayo-Navarrete MI, Ćavar Zeljković S, Tarkowski P, García-Garrido JM. 2024. A dual regulatory role of the arbuscular mycorrhizal master regulator RAM1 in tomato. Journal of Experimental Botany 75, 5021–5036. https://doi.org/10.1093/jxb/erae210