bluuebunny/crossref_metadata_2025_split huggingface dataset:
A STUDY OF MULTICENTRIC RETICULOHISTIOCYTOSIS (LIPOID DERMATOARTHRITIS)
Authors: Ikezawa Zenro , Nakajima Hiroshi 
Published: April 2015

ABSTRACTA case of multicentric reticulohistiocytosis is presented, with typical clinical, roentgenologic, and histologic features. The characteristic giant cells were seen in skin nodules, mucosal nodules, synovial and bony lesions of the joints, and tenosynovial cysts. Corticosteroid therapy produced a subjective improvement, but the arthritis mutilans did not improve.Laboratory studies showed iron‐deficiency anemia, hypertriglyceridemia (hyperprebetalipo‐proteinemia), a low response to the unstimulated and stimulated nitroblue tetrazolium test of neutrophils, and a decreased activity in the glucose‐6‐phosphate dehydrogenase of polimorphonuclear leucocytes before corticosteroid therapy. The histochemical examination of the material in the giant cells showed a lipid (a phospholipid and a neutral fat), and a polysaccharide attached to a protein (a muco‐ or glycoprotein). Electron microscopic studies of the giant cells indicated that their cytoplasm contained numerous dense granules clustered in the vicinity of the Golgi apparatus. In some areas the granules appeared to be developing from the Golgi vesicles and cisternas.It seemed likely that the storage material was produced within the giant cells. We also discussed the pathogenesis of this disorder.
ULTRASTRUCTURAL LOCALIZATION OF PEMPHIGUS ANTIBODIES WITHIN THE EPIDERMIS
Authors: Masutani Mamoru , Shoji Michiko , Taneda Akio , Ogawa Hideoki , Miyazaki Hiroaki 
Published: April 2015

ABSTRACTUltrastructural localization of pemphigus autoantibodies has been studied, using the periodatelysine‐paraformaldehyde fixation method of McLean et al.The deposition of pemphigus autoantibodies was always found in the intercellular spaces of the epidermis, but not observed between the basal surface of basal cells and the basal lamina. Within desmosomes, a linear deposition of antibodies was also found, coresponding to the intermediate dense layer of Odland.
THE ROLE OF LYMPHOID CELLS AND POLYMORPHONUCLEAR LEUKOCYTES IN THE PATHOGENESIS OF PSORIASIS
Authors: Cormane R.H. , Hunyadi J. , Hamerlinck F. 
Published: April 2015

ABSTRACTThe occurrence of antinuclear antibodies (ANA) in eluates of lymphoid cells and polymorphonuclear leukocytes obtained in seven untreated psoriasis patients could be demonstrated by means of the indirect immunofluorescence technique.These antibodies appear to be non‐species‐specific and are mainly directed against the nuclei of the basal cell layer of uninvolved skin, but also ANA directed against the nuclei of epidermal and dermal cells have been encountered.Moreover a significant decrease of circulating lymphoid cells forming rosettes with sheep erythrocytes have been found in the patients studied, suggesting a diminution of the T‐cell control.The immunological mechanisms in psoriasis may therefore be looked upon as being the result of a lack of certain T‐cell suppressor genes facilitating the recognition of nuclear basal cell self‐antigens and a subsequent production of multiclonal anti‐basal cell nuclear antibodies. These antibodies may interfere with the function of membrane bound enzymes which serve as a regulatory epidermal control mechanism.Various exogenous and endogenous factors may help to initiate the immune response leading to a cell‐mediated immune response at the onset of a lesion and an Arthus type reaction located in the upper layers of the epidermis at a later stage of the disease.Consequently the course of events in psoriasis is a self perpetuating inflammatory process.
EVALUATION OF RAST IN ATOPIC DERMATITIS
Authors: Takahashi Isamu , Akiyama Tomio , Yamaura Hideaki , Sasaoka Kazuo , Anan Sadao 
Published: April 2015

ABSTRACTEighty‐two patients with atopic dermatitis were examined for specific IgE antibodies by RAST. A higher number of positive RAST reactions were found in patients with high serum IgE values and with coexisting asthma and/or allergic rhinitis. Positive RAST reactions against mite and house dust were found in many of these patients and even in about one‐half of patients with atopic dermatitis only. The comparisons between intradermal tests and RAST were made with various allergens and the mite extract showed the best agreement between these two tests.
IMMUNOLOGICAL STUDIES OF PITYRIASIS ROSEA (GIBERT)
Authors: Takaki Yasunobu , Koshiyama Yoji , Watanabe Schuichi 
Published: April 2015

ABSTRACTIn order to elucidate the immunological mechanism in the pathogenesis of pityriasis rosea, immunofluorescent studies were performed on sera obtained from forty patients with this disease. Antibodies against the cytoplasm of normal human epidermal cells were demonstrated in the sera of all patients. The antibody titer showed a tendency to increase within 3 weeks after onset of secondary eruptions and then to decrease gradually until the period of recovery. The immunoglobulin class was determined to be IgM. Furthermore, by the direct immunofluorescent technique, deposits of IgM in the epidermal cells of skin lesions were demonstrated in 3 of 6 herald lesions and in 1 of 4 secondary eruptions.It is suggested that anti‐cytoplasmic antibodies produced by some unknown cause may induce the development of secondary eruptions of this disease.
DERMATITIS GANGRENOSA INFANTUM
Authors: Himi Akiko , Ishizaki Hiroshi , Okada Tadahiko 
Published: April 2015

ABSTRACTA case of a 4‐month‐old girl with dermatitis gangrenosa infantum is reported. The lesions were seen on the cheeks and later at the site of blood examination on the earlobes. The patient was successfully treated with antibiotics and then by skin grafting.
COLLOID DEGENERATION OF THE SKIN
Authors: Kawashima Yoshio , Matsubara Tameaki , Kinbara Takeshi , Hirone Takae , Kitamura Kiyotaka , Himi Akiko , Ohkubo Tatsuya 
Published: April 2015

ABSTRACTA patient with multiple nodules on the chin and scalp is presented. Histologically, the nodules were composed of amorphous materials which also completely filled up the whole dermis. Histochemical observation indicated that the amorphous material in the nodule was a kind of glycoprotein. Electron microscopic examination revealed that the material consists of short wavy fibrils, 60 to 100 A in diameter, and of a low electron‐dense amorphous substance. Biochemical examination supported the view that the amorphous material is so‐called sialomucoid. The nodules were finally diagnosed as colloid degeneration of the skin (nodular type).
THE EFFECTS OF CASTRATION AND TESTOSTERONE ADMINISTRATION ON MAST CELLS AND HYDROXYPROLINE IN RAT SKIN
Authors: Hagiyama Masaharu , Arata Jiro , Tanioku Kihei 
Published: April 2015

ABSTRACTIn rat skin of both sexes, significantly higher concentrations of mast cells were observed in the genital area as compared with the skin of the back. The hydroxyproline (HPL) value in the preputial skin was half that in the dorsal skin. The influence of castration and the administration of testosterone on male rat skin mast cells and HPL were investigated. A possible relationship was pointed out between HPL value and the mast cell population of the skin.
EVALUATION OF TONSILLECTOMY AS A TREATMENT FOR PUSTULOSIS PALMAR1S ET PLANTARIS
Authors: Ono Tomomichi 
Published: April 2015

ABSTRACTTwo hundred and eighty five cases of pustulosis palmaris et plantaris (PPP), of which 73 cases (25.6%) underwent tonsillectomy, were studied to evaluate the effectiveness of tonsillectomy as a treatment for PPP. The group of tonsillectomized patients showed significantly better results than a group which received other methods of treatment. A tendency to leukocytosis was observed in PPP which normalized, in many cases, after tonsillectomy. The shorter the period between the onset of PPP and tonsillectomy the better the results, and the quicker the response. A transient worsening of PPP after tonsillectomy did not after the prognosis.In order to determine if tonsillectomy was indicated, an ultrashort wave direct provocation test was conducted in which ultrashort wave was applied to the tonsil. Leukocytosis and worsening of PPP after the test was considered as an indication for tonsillectomy. In some cases, there were considerable changes in plasma Cortisol levels and NBT test values after the provocation test.Improvement of PPP after the Impretol test (one of the annulation tests) in which Impretol was injected into the tonsil, appears to support the focal infection origin of PPP in these cases. There is a possibility that the Impretol test could be used as a treatment for PPP.
A STUDY ON THE CHARACTERIZATION OF CORYNEBACTERIUM ACNES
Authors: Takizawa Kiyohiro 
Published: April 2015

ABSTRACTForty‐nine strains of anaerobic gram‐positive rods were used in a systematic study of their biochemical and physiological reactions and morphological characteristics and were also subjected to gas chromatographic analyses in an effort to classify them as strains of Corynebacterium acnes (C. acnes). The strains were isolated both from lesions in acne vulgaris and from normal skin. According to their biochemical and physiological characters, these 49 strains were divided into six subgroups (Subgroup A–F). They were also separated into two morphological types. The larger of these two types included gram‐positive, unevently staining pleomorphic rods (35 strains); the smaller type contained shorter coccal rods similar to Peptostreptococci (14 strains). The macroscopic appearance of the colonies of both types was the same. All strains of the smaller type showed the same biochemical and physiological characteristics which were of the saccharolytic type (Subgroup B) suggesting a close relationship between the microscopic appearance of the strains and their biochemical and physiological characteristics. Upon microscopical observation, the changing the pH of the media did not cause any transformation of the organism from one type to another. Between pH 6.0 and 6.5 all strains grew well but above pH 8.0 growth wai poor. The gas chromatographic analyses demonstrated that selected sample strains from each of the six subgroups showed the same characteristic chromatograph, suggesting that they could be of the same species, i.e., C. acnes.
A CASE OF SCHÖNLEIN‐HENOCH‐PURPURA: AN IMMUNOPATHOLOGICAL STUDY
Authors: Namba Masako , Kohda Mamoru , Gotoh Masako , Nakagawa Shôjiro , Ueki Hiroaki 
Published: April 2015

ABSTRACTA case of Schönlein‐Henoch‐purpura is presented. The immunofluorescent study of skin lesions of the patient showed granular deposits of IgA and C3 in the blood vessel walls perivascular deposits of plasminogen and diffuse localization of fibrin and fibrinogen in the upper dermis. Complement activation via the alternative pathway through IgA, C3 and plasminogen deposits was suggested.
OBSERVATIONS AND IDENTIFICATION OF BROWN PIGMENT GRANULES FOUND IN THE CHROMATED SKIN A HISTOCHEMICAL STUDY
Authors: Takaiwa Takashi 
Published: April 2015

ABSTRACTA histochemical study of the presence of catecholamine‐containing cells (chromaffin cells) in human skin which had been treated by chromate‐bichromate solution according to Hillarp and Hökfelt then counter‐stained with various methods, was carried out.Small numbers of brown granules were disclosed in the upper part of the chromated normal dermis. They stained green with Giemsa or toluidine blue, showing similar staining properties to the adrenal medulla of rabbits treated in the same way. They were also found in large numbers in nevus pigmentosus and blue nevi, but were rarely seen in Recklinghausen's disease and atopic dermatitis.Although the above‐mentioned brown pigment is not likely to be catecholamine, other possibilities of the nature of the granules are discussed, with special reference to melanin and lipid pigment.
ULTRASTRUCTURAL LOCALIZATION OF IMMUNOGLOBULINS IN THE DERMO‐EPIDERMAL JUNCTION OF PATIENT WITH BULLOUS PEMPHIGOID
Authors: Masutani Mamoru , Ogawa Hideoki , Taneda Akio , Shoji Michiko , Miyazaki Hiroaki 
Published: April 2015

ABSTRACTSkin biopsy specimens were taken from peripheral skin of a bullous eruption of three patients with bullous pemphigoid. For immunoelectron microscopy these tissue blocks were fixed with periodate‐lysine‐paraformaldehyde. This fixation method turned out to be adequate for preservation of both tissue structure and antigenicity. The deposition of immunoglobulin and complements was observed to gradually decrease from the basal surface of the basal cells to the basal lamina. It is suggested that the antigenic substance of bullous pemphigoid is localized along the basal surface of the basal cells.
EPIDERMAL CELL MEMBRANE AS THE ORIGIN OF PEMPHIGUS ANTIGEN
Authors: Nishioka Kiyoshi , Hashimoto Koji , Katayama Ichiro , Kikkawa Akihiko , Sano Shigeharu 
Published: April 2015

ABSTRACTThe substance bound to the pemphigus antibody (P‐Ab), pemphigus antigen, was removed from the surface of the keratinocytes of both human and guinea pigs with high concentrations of trypsin. When keratinocytes were cultured for more than 24 hours, the antigen reappeared on the surface. Furthermore, P‐Ab was recovered from the P‐Ab treated cultured cells by acid treatment.Cell membranes were prepared from human keratinocytes in order to determine whether or not the antigen was situated on their surface. The antigen was also detected on the surface of the cell membrane. As the membrane was found to be able to absorb P‐Ab, the antigen was extracted from the membrane by papain digestion and P‐Ab coated Sepharose column chromatography. The eluate from the column was also able to block P‐Ab activity. At the same time, the culture supernatant of both human and guinea pig keratinocytes was examined for its ability to block P‐Ab activity. The culture supernatant showed a slight ability to block P‐Ab. The origin and nature of the antigen was discussed.
AN APOCRINE TUMOR OF THE GROIN
Authors: Oka Kichiro , Nitto Heita 
Published: April 2015

ABSTRACTA tumor on the groin of a twentyseven year old woman was reported. It consisted of hamartomatous apocrine sweat structures, apocrine cystadenoma and adenoid basal cell epithelioma interconnected within a single connective tissue capsule. To our knowledge, this tumor is the first case of an apocrine tumor having this location and histology with each component representing differentiation of the duct and/or the secretory portion of the apocrine gland to differing degrees in a single lesion. It was hypothesized that the cystadenoma and the basal cell epithelioma had both originated from the latent apocrine hamartoma.
ULTRASTRUCTURE OF MULTIPLE GLOMUS TUMORS
Authors: Tamaki Kunihiko , Hino Haruko , Ishibashi Yasumasa 
Published: April 2015

ABSTRACTGlomus cells of multiple glomus tumors were studied by electron microscopy. Transmission electron microscopic observation revealed that the glomus cells are very similar to smooth muscle cells. Inside the glomus tumor, dome‐shaped and cobblestone‐like structures were found by scanning electron microscope. It is suggested that these structures are composed of endothelial cells and glomus cells.
THE PATTERN OF FIBRINOLYTIC ACTIVITY IN HUMAN SKIN FOLLOWING EXPOSURE TO MIDDLE‐WAVELENGTH ULTRAVIOLET LIGHT OR PSORALEN AND LONG‐WAVE ULTRAVIOLET LIGHT
Authors: Shigemi Fumio , Shiraishi Satoshi , Urano Yoshio , Ohsaki Masafumi 
Published: April 2015

ABSTRACTThis experiment was carried out in order to investigate the time sequence of tissue fibrinolytic activity in human skin following UVB or UVA after topically applicated psoralen using Todd's technique of fibrinolytic autography.Tissue fibrinolytic activity increased in the upper dermis for one to six hours after exposure to psoralen and subseqent UVA, when erythema responses were absent. The return to a normal pattern of fibrinolysis occured in the upper dermis 12–18 hours after treatment with psoralen and UVA, and then psoralen‐UVA‐induced erythema responses began. These responses were maximal 96 hours after treatment with psoralen and UVA, although no fibrinolytic activity was found in the upper dermis. Little change was observed in the lower dermal activity throughout the course of these responses.After UVB irradiation, a transient increase in fibrinolytic activity occured, with a normal pattern of lysis returning in the lower dermis at 12–18 hours. In the lower dermis an increase was observed at one to 6 hours although the activity in the upper dermis had already decreased. UVB erythema responses appeared within this period, were maximal at 6–12 hours, and declined gradually after 12 hours concomitantly with the activity in the upper and lower dermis. The upper dermal activity disappeared at 18 hours, while the lower dermal activity was not found after 72 hours.
ACQUIRED PERIUNGUAL FIBROMA
Authors: Kikuchi Ichiro , Ishii Yoshimitsu , Inoue Shohei 
Published: April 2015

ABSTRACTIt has been confirmed that an acquired periungual fibroma which developed in a 44 year‐old Japanese male X‐ray technician arose either in the dermis proximal to the nail matrix or between the proximal nail fold and the nail matrix. This disease may be a topographical variant of acquired digital fibrokeratoma.
EXPERIMENTAL BEHÇET'S DISEASE AND ULTRASTRUCTURAL X‐RAY MICROANALYSIS OF PATHOLOGICAL TISSUES
Authors: Hori Yoshiaki , Miyazawa Shichiro , Nishiyama Shigeo , Miyata Mikio , Ishikawa Satoshi 
Published: April 2015

ABSTRACTEnvironmental chemicals (benzene hexachloride(BHC) and dichlor diphenyl trichlor ethane(DDT) as organic chlorides, Sumithion as an organic phosphorated compound and copper powder) were mixed together and administered orally to pedigreed Pitman‐Moor's strain miniature swine for a period of about one year. Oral aphthae, folliculitis, cutaneous and subcutaneous nodules, genital ulcers, hyperemia of conjunctiva and intestinal ulcers were observed in all treated animals.Biopsy and autopsy specimens obtained from the experimental animals showed typical histopathological findings of human Behçet's disease.By ultrastructural X‐ray microanalysis of peripheral neutrophils, vascular endothelial cells and infiltrated cells in affected lesions obtained from the experimental animals, highly significant amounts of chlorine and phosphorus were detected, mainly in the lysosomes.The incidence of Behçet's disease has been reported in countries where pesticides made from organic chlorine, organic phosphorus, arsenic and copper compounds have been used in massive amounts.Therefore, our findings suggest that these environmental chemicals play a main role in the pathogenesis of Behçet's disease.
REFLECTION ULTRAVIOLET PHOTOGRAPHY IN DERMATOLOGY
Authors: Kikuchi Ichiro , Idemori Masahiro , Uchimura Hideo , Inoue Shohei 
Published: April 2015

ABSTRACTOur experiences in reflection ultraviolet (UV) photography with special and conventional equipment are described. Although special lenses have advantages over conventional lenses such as in the transmission of ultraviolet radiation, it is established that reflection UV photography is possible with usual 35 mm single lens reflex cameras and 60 mm twin lens reflex cameras. Photography of semitranslucent material revealed that ultraviolet radiation is reflected from upper layers than visible light.
COMPLEMENT FIXING ANTIBODY TO VARICELLA‐ZOSTER VIRUS IN DIFFERENT AGE GROUPS
Authors: Hata Seiichiro 
Published: April 2015

ABSTRACTComplement fixing antibody to varicella‐zoster virus was studied in sera from 619 persons not suffering from varicella or herpes zoster.The average incidence of the antibody was 23%. There were three points of the high incidence of the antibody, observed in the 10–14, 36–39 and over 60 years age groups which corresponds well to the frequency of varicella and herpes zoster found in or near the above groups.
STATISTICAL SURVEY OF 100 CASES OF SPOROTRICHOSIS
Authors: Kariya Hideo , Iwatsu Tokio 
Published: April 2015

ABSTRACTA statistical survey of 100 cases of sporotrichosis at the Dermatological Clinic of Chiba University Hospital from May, 1965 to June, 1975 was reported. The proportion of sporotrichosis cases to the total number of outpatients and to the total dermatomycoses was 0.14% and 1.8%. It has increased gradually since 1969. Greater percentage of cases fell into the less than 11 year old or more than 40 year old age groups. Females were more affected than males. Half of the cases occured in farmers. Geographic distribution was remarkable. 44 cases had a history of injury. 62 cases showed the cutaneous lymphatic type and 38 cases showed the localized cutaneous type. The face and upper extremities were the most affected. The sporotrichin test was positive in 41 of 42 cases. The causative organism was demonstrated in tissue sections in 97 of 99 cases.
CYTOMORPHOGENESIS AND MELANOSOME TRANSFER DURING MALIGNANT TRANSFORMATION IN MELANOCYTES AND MELANOMA CELLS
Authors: Jimbow Kowichi 
Published: April 2015

ABSTRACTThe relation between cellular differentiation and associated cytomorphogenesis and secretion in pigment cells was studied by using scanning and transmission electron microscopes. Cytomorphogenesis and the related transfer of melanosomes was compared in epidermal melanocytes of guniea pigs and melanoma cells of mice grown in culture.Scanning electron microscopic studies showed that melanocytes and melanoma cells have a different surface structure, which is modified according to cellular differentiation. The melanocytes and their dendrites possess poorly developed villous pods or microvillies. The dendrites secrete melanosomes extracellularly in the vicinity or on the surface of keratinocytes. Melanoma cells, on the other hand, reveal well developed villous pods which are often directly attached to the surface of the dish. Thus villous pods do not appear to play any part in the melanosome transfer. Also, the melanoma cells do not transfer melanosomes as often as do the melanocytes. Furthermore melanosomes are transferred to keratinocytes after being secreted extracellularly.Transmission electron microscopy indicated that the melanocytes and melanoma cells contain 5 nm filaments responsible for anchoring the cells to the dish in vitro. Epidermal melanocytes also contain numerous 10 nm filaments that are motile organelle and induce a motive force for melanosome transfer.
APPLICATION OF PROTEIN A TO THE STUDY OF PEMPHIGUS ANTIBODIES
Authors: Miyagawa Sachiko , Hashimoto Ken , Judd Kenneth , Lever Walter F. 
Published: April 2015

ABSTRACTFITC‐labeled staphylococcal protein A was used to detect pemphigus antibodies. The results showed that protein A was specific in the systems tested, although it was not as sensitive as FITC‐anti IgG in indirect immunofluorescence tests. Protein A‐adsorbent was used to isolate IgG from pemphigus serum samples. Recovery of pemphigus antibody activity was a mean of 57.1 percent, as high as that of DEAE chromatography. We consider protein A to be an alternative to monospecific anti IgG for detection of pemphigus antibodies and for purification of IgG from pemphigus sera.
THE MAJOR HISTOCOMPATIBILITY COMPLEX IN PSORIASIS VULGARIS
Authors: Ozawa Akira , Matsuo Itsuro , Niizuma Kan , Ohkido Muneo , Nose Yoshisuke , Inouye Hiroo , Tsuji Kimiyoshi 
Published: April 2015

ABSTRACTHLA‐C typing was performed on 40 unrelated Japanese patients with psoriasis vulgaris. The patient group consisted of 22 patients who had psoriatic HLA antigens i.e., B13 in seven cases, B17 in one case and B37 in 14 cases; and 18 patients who had none of these specific HLA antigens. HLA‐C typing was also performed on 40 unrelated healthy Japanese controls. Cw6 was found in 55.0% of the patients as compared with 5.0% of the healthy controls. All patients (21 cases) who had either B13 or B37 were positive for Cw6. Both controls who were positive for Cw6 also had B37. These results suggest the possibility of a linkage disequilibrium between Cw6 from locus C and B13 and B37 from locus B, and/or an association between Cw6 and susceptibility to psoriasis.