bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pemanfaatan Tepung Daun Kelor Pada Pembuatan Kerupuk Ikan Dalam Meningkatkan Kapasitas Ibu Rumah Tangga Di Wilayah Kelurahan Berua Kecamatan Biringkanaya Kota Makassar
Authors: Sukmawati Sukmawati , Rauf Suriani 
Published: July 2024

Masalah anemia pada ibu hamil dan stunting pada anak balita masih menjadi masalah gizi bagi warga Kelurahan Berua yang perlu penanganan secara serius. Salah satu strategi yang dapat dilakukan adalah mengimplementasikan hasil riset berupa teknologi/sains dalam bentuk kegiatan pengabdian masyarakat berupa pelatihan pemanfaatan tepung daun kelor pada pembuatan kerupuk ikan dengan penambahan tepung daun kelor. Tujuan pengabdian masyarakat untuk meningkatkan pengetahuan dan keterampilan kader dan ibu rumah tangga untuk mengolah kerupuk ikan dengan penambahan tepung daun kelor. Metode yang dilakukan berupa pelatihan cara membuat kerupuk ikan. Materi pelatihan terdiri dari teori dan praktek. Indikator keberhasilan pelatihan dilihat dari peningkatan pengetahuan dan keterampilan setelah pelatihan yang diperoleh melalui pre-post test. Setalah pelatihan peserta diberi alat dan bahan makanan untuk praktek mandiri dirumah. Hasil pengabmas, sebelum pelatihan hanya (40%) yang menjawab soal dengan benar, dan setalah pelatihan semua peserta (100%) menjawab soal dengan benar. Hasil post test sebelum praktek hanya 1 orang (5%) peserta yang terampil dan setelah pelatihan semua peserta yaitu 20 ibu (100%) terampil membuat kerupuk ikan dengan penambahan tepung daun kelor. Hasil praktek mandiri semua kelompok telah membuat video kegiatan yang telah dikirim kepada ketua tim pengabdi. Kesimpulan setelah pelatihan terjadi peningkatan pengetahuan dan keterampilan peserta.
Kata kunci: Daun Kelor, Kerupuk Ikan, Kapasitas Ibu
Pemenuhan Ketahanan Pangan Keluarga melalui Pembuatan Vertical Garden
Authors: Habib Akbar , Intan Dian Retno , Lubis Wildani , Kabeakan Nana Trisna Mei Br , Purba Khairul Fahmi , Ginting Litna Nurjannah 
Published: July 2024

Semakin meningkatnya populasi penduduk dan adanya dorongan untuk hidup sehat tidak sejalan dengan ketersediaan lahan pertanian. Kecenderungan konversi lahan pertanian menyebabkan terjadinya kesulitan penurunan dalam produksi komoditas pertanian. Selain itu, terdapat juga kekhawatiran dalam penggunaan teknik budidaya secara konvensional berupa pengaplikasian pupuk dan pestisida kimia. Alasan-alasan inilah yang menjadi awal dari munculnya konsep prosumer. Prosumer adalah konsep ketika orang memiliki kemampuan untuk memproduksi makanan mereka sendiri dengan menggunakan sumber daya yang tersedia. munculnya konsep prosumer membantu daerah perkotaan di banyak negara untuk meningkatkan produksi komoditas pertanian dan menjaga keberlanjutannya. Perumahan di Kota Medan merupakan daerah padat penduduk yang setiap rumah jarang sekali memiliki pekarangan luas. Pekarangan biasanya hanya terbatas atau cukup dan dibuat hanya untuk keperluan memarkirkan kendaraan saja. Pekarangan yang sempit ini dapat dibuat menjadi multifungsi sebagai tempat bercocok tanam dengan menggunakan vertical garden. Budidaya tanaman sendiri ini dalam rangka untuk pemenuhan kebutuhan asupan sayuran keluarga untuk mencapai ketahanan pangan keluarga dengan menyediakan sendiri. Adanya kegiatan ini diharapkan tidak hanya dapat memenuhi kebutuhan asupan sayuran mitra saja, diharapkan juga mampu mengurangi pengeluaran rumah tangga dan lebih luasnya lagi mitra dapat mengembangkannya sampai tercapai ketahanan pangan keluarga.
Wet Sliding Wear Investigation of Ni-Based Coating for Piston Cylinder
Authors: Rukhande Sanjay , Amrutkar Siddhant , Joshi Abhishek , Jethwa Yash , Gujar Kaushal , Siddiqui Aqleem , Kudachi Badal 
Published: July 2024

This paper presents the investigation of sliding wear of nickel-based coatings for piston cylinder application. Wear and friction between cylinder walls and pistons is a critical issue in industries like power generation, aerospace, and nuclear plants, contributing significantly to engine losses. NiCrBSiFeC has been coated on steel by high velocity oxy fuel thermal process and investigated on tribometer for sliding wear as per ASTM G99 standard. The design of experiments was done by considering normal load, speed and track diameter as variable with three level. The wear tracks are analyzed using electro discharge spectrum (EDS) analysis and scanning electron microscope (SEM) images. The specific Mass Wear Rate (MWR) and coefficient of frictions are compared for coated and uncoated conditions. The results indicated the significant improvement in the wear life for the coated sample with mild abrasive and delaminated wear mechanism.
Comparing 2-day vs 3-day flu-CY lymphodepleting regimens for CD19 CAR T-cell therapy in patients with non-hodgkin’s lymphoma
Authors: Frame David G. , Geer Marcus , Kasha Salena , Markstrom Denise , Scappaticci Gianni , Feeney Tate , Hayduk Andrew , Mansoor Hilary M. , Oberfeld Avery , D’Antonio Hannah , Anand Sarah , Choi Sung Won , Maciejewski John , Pawarode Attaphol , Riwes Mary Mansour , Tewari Muneesh , Magenau John , Ghosh Monalisa 
Published: July 2024

IntroductionLymphodepleting chemotherapy (LDC) is critical to CAR T-cell expansion and efficacy. Despite this, there is not a consensus in the literature regarding the optimal LDC regimen, including dose and frequency.MethodsWe retrospectively reviewed consecutive patients at a single institution that received LDC prior to treatment with the CD19 directed CAR T-cell products axicabtagene ciloleucel and tisagenlecleucel. Patients treated at our center received fludarabine 30 mg/m2 and cyclophosphamide 500 mg/m2 for 3 consecutive days prior to May 2019. After this timepoint patients routinely received fludarabine 40 mg/m2 and cyclophosphamide 500 mg/m2 for 2 consecutive days. Clinical data from each cohort were obtained from the electronic medical record and compared for differences in CAR T-cell efficacy and toxicity.ResultsFrom June 2018 to August 2023, LDC was given to 92 patients prior to CD19 directed CAR T-cell therapy for relapsed non-Hodgkin’s lymphoma. Twenty-eight patients received a 3-day regimen, and 64 patients received a 2-day regimen. In the total cohort, 75% of patients received axicabtagene ciloleucel and 25% received tisagenlecleucel. The overall response rates in both the 2-day regimen group and the 3-day regimen group were similar (69% vs 75%, p= 0.21) as were the complete response rates (50% vs 54%, p=0.82). There were no significant differences between the 2-day and 3-day regimens for grade 2–4 cytokine release syndrome (55% vs 50%, p=0.82), grade 2–4 immune effector cell associated-neurotoxicity syndrome (42% vs 29%, p=0.25), or time to resolution of neutropenia or thrombocytopenia. The rate of prolonged platelet recovery lasting greater than 60 days was higher with the 3-day regimen (9% vs 27%, p=0.026).DiscussionAs the number of patients eligible for CAR T-cell therapy continues to increase, optimizing each component of therapy is necessary. We show that a 2-day regimen of LDC with fludarabine and cyclophosphamide is feasible without significant impact on CAR T-cell efficacy or toxicity. Prospective studies are necessary to further determine the most effective LDC regimen.
Development and evaluation of a triplex droplet digital PCR method for differentiation of M. tuberculosis, M. bovis and BCG
Authors: Qu Yao , Liu Mengda , Sun Xiangxiang , Liu Yongxia , Liu Jianzhu , Hu Liping , Jiang Zhiqiang , Qi Fei , Nan Wenlong , Yan Xin , Sun Mingjun , Shao Weixing , Li Jiaqi , Sun Shufang , Zhang Haobo , Fan Xiaoxu 
Published: July 2024

IntroductionTuberculosis, caused by Mycobacterium tuberculosis complex (MTBC), remains a global health concern in both human and animals. However, the absence of rapid, accurate, and highly sensitive detection methods to differentiate the major pathogens of MTBC, including M. tuberculosis, M. bovis, and BCG, poses a potential challenge.MethodsIn this study, we have established a triplex droplet digital polymerase chain reaction (ddPCR) method employing three types of probe fluorophores, with targets M. tuberculosis (targeting CFP-10-ESAT-6 gene of RD1 and Rv0222 genes of RD4), M. bovis (targeting CFP-10-ESATs-6 gene of RD1), and BCG (targeting Rv3871 and Rv3879c genes of ΔRD1), respectively.ResultsBased on optimization of annealing temperature, sensitivity and repeatability, this method demonstrates a lower limit of detection (LOD) as 3.08 copies/reaction for M. tuberculosis, 4.47 copies/reaction for M. bovis and 3.59 copies/reaction for BCG, without cross-reaction to Mannheimia haemolytica, Mycoplasma bovis, Haemophilus parasuis, Escherichia coli, Pasteurella multocida, Ochrobactrum anthropi, Salmonella choleraesuis, Brucella melitensis, and Staphylococcus aureus, and showed repeatability with coefficients of variation (CV) lower than 10%. The method exhibits strong milk sample tolerance, the LOD of detecting in spike milk was 5 × 103 CFU/mL, which sensitivity is ten times higher than the triplex qPCR. 60 clinical DNA samples, including 20 milk, 20 tissue and 20 swab samples, were kept in China Animal Health and Epidemiology Center were tested by the triplex ddPCR and triplex qPCR. The triplex ddPCR presented a higher sensitivity (11.67%, 7/60) than that of the triplex qPCR method (8.33%, 5/60). The positive rates of M. tuberculosis, M. bovis, and BCG were 1.67, 10, and 0% by triplex ddPCR, and 1.67, 6.67, and 0% by triplex qPCR, with coincidence rates of 100, 96.7, and 100%, respectively.DiscussionOur data demonstrate that the established triplex ddPCR method is a sensitive, specific and rapid method for differentiation and identification of M. tuberculosis, M. bovis, and BCG.
Causal associations of gut microbiota and pulmonary tuberculosis: a two-sample Mendelian randomization study
Authors: Lu Zhongkui , Xu Weiping , Guo Yidi , He Fang , Zhang Guoying 
Published: July 2024

BackgroundThe prevalence of pulmonary tuberculosis (PTB) as an infectious disease continues to contribute significantly to global mortality. According to recent studies, the gut microbiota of PTB patients and healthy controls (HCs) show significant disparities. However, the causal relationship between them has yet to be elucidated.MethodsWe conducted a study using Mendelian Randomization (MR) to explore the potential causal link between gut microbiota and pulmonary tuberculosis (PTB). The summary statistics of the gut microbiota were acquired from the MiBioGen consortium, while data on PTB were sourced from pheweb.jp. A range of statistical methodologies were employed to evaluate causality, encompassing inverse variance weighting (IVW), MR-Egger, weighted median (WM), weighted model, and simple model. We utilized instrumental variables (IVs) that have a direct causal relationship with PTB to annotate SNPs, aiming to discover the genes harboring these genetic variants and uncover potential associations between host genes and the microbiome in patients with PTB.ResultsAmong the 196 bacterial traits in the gut microbiome, we have identified a total of three microbiomes that exhibit a significant association with PTB. The occurrence of Dorea (P = 0.0458, FDR-adjusted P = 0.0458) and Parasutterella (P = 0.0056, FDR-adjusted P = 0.0168) was linked to an elevated risk of PTB, while the presence of Lachnoclostridium (P = 0.0347, FDR-adjusted P = 0.0520) demonstrated a protective effect against PTB. Our reverse Two-Sample Mendelian Randomization (TSMR) analysis did not yield any evidence supporting the hypothesis of reverse causality from PTB to alterations in the intestinal flora.ConclusionWe have established a connection between the gut microbiota and PTB through gene prediction analysis, supporting the use of gut microecological therapy in managing PTB and paving the way for further understanding of how gut microbiota contributes to PTB’s development.
High charge of cerebroid nests in nodular melanomas predicts tumor aggressiveness and high mutational tumoral burden: a pilot study
Authors: Caramaschi Stefania , Mangogna Alessandro , Bertoni Laura , Manfredini Marco , Farnetani Francesca , Parente Paola , Attino Vito , Cazzato Gerardo , Salviato Tiziana , Pellacani Giovanni , Reggiani Bonetti Luca 
Published: July 2024

PurposeEven today, melanoma is a highly aggressive neoplasm with a high mortality rate. The nodular type is very aggressive and has cerebroid nests of melanocytes (CNMs) at the growth edge, morphologically similar to the poorly differentiated neoplastic epithelial cell clusters described in colorectal, breast, and endometrioid endometrial cancers.Patients and methodsWe selected 25 nodular melanomas (NMs) with known molecular profiles, of which the entire paraffin-embedded lesion was available. We counted CNMs under a microscopic at a magnification of 20x (i.e., a microscopic field with a major axis of 1 mm). Based on the number of CNMs in the area, melanomas were classified into three groups: G1 (CNMs ranging from 0 to 4), G2 (CNMs ranging from 5 to 9), and G3 (CNMs ≥ 10). The presence of CNMs and their counts were compared with molecular and histopathological data.ResultsSeventeen (NMs) were grouped as G1 (68%), 5 as G2 (20%), and 3 as G3 (12%) based on CNMs count. The presence of CNMs correlated with epithelioid cell morphology (p &lt; 0.05), Clark IV and V levels (p &lt; 0.05), vascular invasion (p &lt; 0.05), and biological mutants (p &lt; 0.05). Melanomas with ≥ 10 CNMs more frequently show ulceration (p &lt; 0.02) and the BRAF V600E mutation (p &lt; 0.02).ConclusionCNMs count has a predictive role regardless of tumor size; their association with the BRAF V600E mutation suggests their predictive significance in response to biologics. However, further investigations are needed to strengthen this hypothesis.
Effect of resistance exercise on physical fitness, quality of life, and fatigue in patients with cancer: a systematic review
Authors: Zhang Qiuhua , Gao Yanan , Wang Wenjun , Zhao Xiaoguang , Yu Jiabin , Huang Huiming 
Published: July 2024

ObjectiveThe purpose of this study is to conduct a systematic review to assess the effects of different forms of resistance exercises (resistance exercise, resistance exercise combined with aerobic exercise, and resistance exercise combined with other exercises) on physical fitness, quality of life (QOL), and fatigue of patients with cancer.MethodsWe conducted a systematic review using the Cochrane Handbook for Systematic Reviews of Interventions guidelines. We searched PubMed, Web of Science, and Scopus databases for the studies from the establishment of the database to September 2023, including randomized controlled trials and clinical trials that evaluated the effects of different resistance exercise on physical fitness, QOL, and fatigue in all patients with cancer. Two reviewers independently assessed the quality of all the included studies using the Cochrane Handbook for Systematic Reviews of Interventions and MINORS scale. We divided the intervention into three types: resistance exercise, resistance exercise combined with aerobic exercise, and resistance exercise combined with other exercises.ResultsIn total, 48 studies (3,843 participants) met the inclusion criteria. The three exercise intervention forms have significant effects on physical fitness and QOL, but the improvement effect on fatigue is not clear. A total of 34 studies reported significant and beneficial effects of resistance exercise on physical fitness across all types of cancer. There were 28 studies that reported significant or borderline improvement effects of resistance on QOL, and only 10 studies reported significant effects of resistance exercise interventions on fatigue improvement in patients with cancer.ConclusionsResistance exercise, resistance exercise combined with aerobic exercise, and resistance exercise combined with other exercises all have a positive effect on improving fitness and QOL in patients with cancer. Resistance exercise has an advantage in improving muscle strength, while combined resistance exercise has an advantage in improving QOL; however, there are no consistent findings in improving fatigue, although low-intensity resistance exercise is effective.Systematic review registrationwww.inplasy.com, identifier INPLASY2023110034.
Revealing the role of metformin in gastric intestinal metaplasia treatment
Authors: Hu Ruoyu , Xue Xia , Sun Xiangdong , Mi Yang , Wen Huijuan , Xi Huayuan , Li Fuhao , Zheng Pengyuan , Liu Simeng 
Published: July 2024

ObjectiveGastric intestinal metaplasia (IM) is a precancerous stage associated with gastric cancer. Despite the observed beneficial effects of metformin on IM, its molecular mechanism remains not fully elucidated. This study aims to reveal the effects and potential mechanisms of metformin in treating IM based on both bioinformatics and in vivo investigations.MethodsThe seven public databases (GeneCards, DisGeNET, OMIM, SuperPred, Pharm Mapper, Swiss Target Prediction, TargetNet) were used in this work to identify targeted genes related to intestinal metaplasia (IM) and metformin. The shared targeted genes between metformin and IM were further analyzed by network pharmacology, while the interactions in-between were investigated by molecular docking. In parallel, the therapeutic effect of metformin was evaluated in IM mice model, while the core targets and pathways effected by metformin were verified in vivo.ResultsWe screened out 1,751 IM-related genes and 318 metformin-targeted genes, 99 common genes identified in between were visualized by constructing the protein-protein interaction (PPI) network. The top ten core targeted genes were EGFR, MMP9, HIF1A, HSP90AA1, SIRT1, IL2, MAPK8, STAT1, PIK3CA, and ICAM1. The functional enrichment analysis confirmed that carcinogenesis and HIF-1 signaling pathways were primarily involved in the metformin treatment of IM. Based on molecular docking and dynamics, we found metformin affected the function of its targets by inhibiting receptor binding. Furthermore, metformin administration reduced the progression of IM lesions in Atp4a−/− mice model significantly. Notably, metformin enhanced the expression level of MUC5AC, while inhibited the expression level of CDX2. Our results also showed that metformin modulated the expression of core targets in vivo by reducing the activity of NF-κB and the PI3K/AKT/mTOR/HIF-1α signaling pathway.ConclusionThis study confirms that metformin improves the efficacy of IM treatment by regulating a complex molecular network. Metformin plays a functional role in inhibiting inflammation/apoptosis-related pathways of further IM progression. Our work provides a molecular foundation for understanding metformin and other guanidine medicines in IM treatment.
Ethnobotanical study of traditional medicinal plants used to treat human ailments in West Shewa community, Oromia, Ethiopia
Authors: Beressa Tamirat Bekele , Gadisa Diriba Alemayehu , Mammo Siraj , Umeta Gurmu Tesfaye , Meskele Lemma Bose , Gudeta Biruk Mosisa , Taye Getu Melesie 
Published: July 2024

Introduction: Plants have formed the basis of traditional medicine (TM) systems, which have been used for thousands of years. According to reports, one-quarter of the commonly used medicines contain compounds isolated from plants. This study aims to identify and document the plants for ethno-pharmacological use by the indigenous communities of West Shoa Zone, Oromia region, Ethiopia.Methods: The cross-sectional study was conducted from November 2020 to November 2021 in West Shewa Zone, Oromia Region, Ethiopia. The ethnobotanical data was collected from Ejere District, Ada Berga District, Dandi District, Ambo District, Ambo Town, Toke Kutaye District, and Bako Tibe District. A descriptive statistical method (percentage and/or frequency) was employed to summarize ethnobotanical data. Moreover, the informant consensus factor was computed. Microsoft Excel spreadsheet software (Microsoft Corporation, 2016) and SPSS (version 25) were used to organize and analyze the data.Result: In the study area, a total of 51 families of medicinal plants with 108 Species were identified. Fabaceae 8 species, Asteraceae, Solanaceae and Lamiaceae each with 6 species and Cucurubitacieae 5 species were the frequently reported medicinal plants. The leaf (57.2%) was the most widely used medicinal plant parts, and oral administration (56.5%) was the most cited route of administration. In the present study, most of the medicinal plants were used fresh, which was (75%) and the most common disease the healers treated was gastrointestinal disease, followed by skin disease and febrile illness. The major threat to medicinal plants in the study area was agricultural expansion, which was reported by 30.6% of the respondents. The study area was rich in medicinal plants, Fabaceae which commonly used family.Conclusion: Most of the medication prepared by the traditional healers was taken orally and derived from the leaf part of the medicinal plant. Since this research is a preliminary study which will be used as a base for further study. The efficacy and safety of the medicinal plant claim should be studied in the future.
Exploring the sustained impact of the Mindfulness-Based Stress Reduction program: a thematic analysis
Authors: Yavuz Sercekman Meltem 
Published: July 2024

IntroductionThis study aimed to explore the time-varying impact of the Mindfulness-Based Stress Reduction (MBSR) program, specifically examining its effects on various variables 3 months, 1 year, and 3 years after program completion. Additionally, the study aimed to identify the barriers and facilitators in maintaining mindfulness practice over time and the preferred mindfulness practices among participants in three distinct time groups.MethodsThe study utilized a qualitative research design, conducting semi-structured interviews with 45 participants who had completed the 8-week MBSR program at different time points. Thematic analysis was employed to analyze the qualitative data obtained from the interviews, allowing for the identification of key themes and patterns.ResultsThe findings revealed that the effectiveness of the MBSR program varied at different times and across different variables. Immediately after completing the program, participants experienced a significant decrease in stress levels and an increase in awareness. One year later, the program continued to have positive effects on inner calm, coping mechanisms, and relationships. Three years after completing the program, its long-term impact was observed in the adoption of a mindful lifestyle, increased compassion and kindness, and ongoing personal growth.DiscussionThe study highlights the transformative potential of the MBSR program beyond short-term symptom relief. The long-term effects observed in overall wellbeing emphasize the sustained efficacy of mindfulness-based interventions. The identified barriers and facilitators in maintaining mindfulness practice provide insights for program implementation and individual engagement. By understanding the long-term impact and preferences of participants, tailored interventions can be developed to maximize the benefits of the MBSR program for individuals over time.