bluuebunny/crossref_metadata_2025_split huggingface dataset:
Carbohydrate Markers in Colon Carcinoma
Authors: Szajda Sławomir Dariusz , Jankowska Anna , Zwierz Krzysztof 
Published: June 2013

Spontaneously mutated multiple oncogenes and/or tumor suppressor genes in colon epithelial cell and its progeny, may cause proliferation out of control and create benign colon neoplasm (colon polyp). If additional mutations involve genes responsible for cell adhesion and movement, aberrant epithelial cells may become malignant (colon cancer) and invade surrounding and remote tissues, creating secondary tumors called metastases.Incidence of colorectal cancer dramatically increases at 50–65 year of age. In Europe in 2006 colorectal cancer consisted 12.9% of all cancers and caused 207 400 deaths.To laboratory detection and monitoring of colon cancer are used tumor markers. Tumor markers are substances produced by the body in response to cancer, or by cancer tissue itself. Glycoconjugate markers for colon cancer include aberrant: mucins covering the surface of the colon epithelial cells, cadherins, selectins and Ig –like adhesion molecules mediating cell‐cell adhesion, integrins and integral membrane proteoglycans responsible for adhesion of colon epithelial cells to extracellular matrix, glycoconjugate components of ECM, as well as lysosomal membrane glycoproteins and exoglycosidases.Detection of colon cancer at early non malignant stage is crucial in its prevention and eradication. As colon cancer is the effect of accumulation many somatic mutations in oncogens, supressors, mismatch repair genes and many genes responsible for posttranslational modifications of proteins, multidirectional approach should be applied for its detection. A glycobiological approach to diagnosis and treatment of colorectal cancer should be directed to detection changes in glycosylation accompanying every step of colon cancer progression, and correlation between changes in glycosylation and tumor progression.
Elevation in Peripheral Blood Circulating Tumor Cell Number Correlates with Macroscopic Progression in UICC Stage IV Colorectal Cancer Patients
Authors: Molnar Bela , Floro Lajos , Sipos Ferenc , Toth Bernadett , Sreter Lydia , Tulassay Zsolt 
Published: June 2013

Aims: Cytokeratin(CK) based real‐time RT‐PCR assays (QRT‐PCR) are now available for peripheral blood circulating tumor cell (CTC) evaluations in colorectal cancer(CRC) patients. Results are non‐existent for the application of these techniques to the determination of progression and therapy response in Dukes stage D CRC patients.Patients and methods: Each month 30 ml peripheral blood of 30 Dukes D patients (17 with progression) were drawn. CEA, CA19‐9, CA72‐A and TPA‐M determinations were made. CK20, thymidilate synthase(TS) QRT‐PCR was performed, as well. Buffy coat was used for immunmagnetic cancer cell isolation and CTC counting. Correlation between elevated CTC and macroscopic progression within 3 months was determined by Chi2 test.Results: Microscopic CTC single cell, doublet, cluster number were found in correlation with CK20 QRT‐PCR results (p <
0.01). There was a significant increase in microscopic CTC number, CK20 and decrease in TS QRT‐PCR levels (p < 0.05) in
the peripheral blood of the non‐responder as compared to responder patients. Elevation of the CTC was in significant correlation
with macroscopic progression of the disease in 3 months (p < 0.01).Conclusions: CTC number reflects the chemotherapeutic sensitivity of CRC patients. Elevation of circulating tumor cell number in peripheral blood is in correlation with macroscopic progression.
A novel Multiple-Marker Method for the Early Diagnosis of Oral Squamous Cell Carcinoma
Authors: Ries Jutta , Mollaoglu Nur , Toyoshima Takeshi , Vairaktaris Eleftherios , Neukam Friedrich W. , Ponader Sabine , Nkenke Emeka 
Published: June 2013

Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC).Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples.Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages.Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.
Serum Ferritin Is Inversely Correlated with Serum Adiponectin Level: Population-Based Cross-Sectional Study
Authors: Ku Bon-Jeong , Kim Seul-Young , Lee Tae-Yong , Park Kang-Seo 
Published: June 2013

Background: The serum concentrations of ferritin and adiponectin are associated with several metabolic disorders and have been used as predictors of insulin resistance and metabolic syndrome. But there have been no reports demonstrating a direct correlation between serum ferritin and adiponectin levels. We performed this study to evaluate the association between serum ferritin and adiponectin concentrations.Subjects and methods: We evaluated a total of 995 subjects from the Korea Rural Genomic Cohort Study in a population-based cross-sectional study. Extensive clinical and laboratory measurements, including adiponectin and ferritin concentrations, were recorded.Results: Univariate analysis revealed that the serum adiponectin level was correlated with age, sex, BMI, diastolic blood pressure, triglyceride, HDL-cholesterol, fasting blood glucose, fasting insulin, HOMA-IR, hs-CRP, and ferritin. Multivariate analysis demonstrated that the adiponectin concentration was correlated with age, BMI, fasting glucose, hs-CRP, and ferritin. The ferritin concentration was the most powerfully associated with serum adiponectin. In non-diabetic subgroups, the adiponectin level was correlated with BMI, triglyceride, HDL-cholesterol, fasting glucose, and ferritin level in multivariate analysis. In diabetic subgroups, the adiponectin level was correlated with BMI, triglyceride, hs-CRP, and ferritin level in multivariate analysis.Conclusions: The serum adiponectin concentration was correlated with conventional clinical variables, but the most powerfully associated factor was the serum ferritin level.
CD14CD16 Monocyte Subset Levels in Heart Failure Patients
Authors: Barisione Chiara , Garibaldi Silvano , Ghigliotti Giorgio , Fabbi Patrizia , Altieri Paola , Casale Maria Carla , Spallarossa Paolo , Bertero Giovanni , Balbi Manrico , Corsiglia Luca , Brunelli Claudio 
Published: June 2013

Our aim was to define the distribution of monocyte subsets in a cohort of congestive heart failure (CHF) patients, to verify whether increased severity of CHF is linked to the expansion of specific monocyte subsets, and finally to investigate the relationship between monocyte subset relative frequencies, laboratory parameters of inflammation, and monocyte ACE expression.Thirty consecutive CHF patients and 26 healthy control subjects were evaluated for peripheral blood monocyte expression of CD14, CD16 and CD143 (ACE) by flow-cytometry, and for endothelial-derived soluble CD146 levels by ELISA.CD14++CD16+frequency was significantly higher in CHF patients than in Controls (%, median value and IQ) (12.3, 8.7–14.8 vs 5.9, 4.7–6.9, p++CD16+levels. Frequencies of CD14+CD16+monocytes were significantly lower in CHF patients as compared to Controls, and negatively correlated with levels of soluble CD146 (r= −0.529; p 0.048).In conclusion, monocytic CD14++CD16+frequency and CD143 levels are increased and reflect disease status and progressive cardiac deterioration in CHF patients. The CD14+CD16+subset is depleted in CHF and is linked to endothelial damage in this group of patients.Although the question of whether differences in monocyte CD14CD16 expansion are causal or whether they represent a marker of HF progression which is potentially relevant for risk prediction remains unanswered, we believe that our data represent an important tool for exploring the role of selective inflammatory pathways in CHF progression.
Matrix Metalloproteinases 2 and 3 Gene Polymorphisms and the Risk of Target Vessel Revascularization after Percutaneous Coronary Intervention: Is There Still Room for Determining Genetic Variation of MMPs for Assessment of an Increased Risk of Restenosis?
Authors: Verschuren J.J.W. , Sampietro M.L. , Pons D. , Trompet S. , Ewing M.M. , Quax P.H.A. , de Knijff P. , Zwinderman A.H. , de Winter R.J. , Tio R.A. , de Maat M.P. , Doevendans P.A.F.M. , Jukema J.W. 
Published: June 2013

Objective: Mixed results have been reported of matrix metalloproteinases (MMP) and their association with restenosis after percutaneous coronary intervention (PCI). The current study examines whether multiple single nucleotide polymorphisms (SNPs), covering the full genomic region of MMP2 and MMP3, were associated with restenosis in the GENDER study population.Methods and results: The GENetic DEterminants of Restenosis (GENDER) study enrolled 3104 consecutive patients after successful PCI. The primary endpoint was clinical restenosis, defined as target vessel revascularization (TVR), occurring in 9.8% of the patients. From the Hapmap database, 19 polymorphisms of MMP2 and 11 of MMP3 were selected. Furthermore, in a subpopulation, a genome-wide association analysis (GWA) was performed. No significant association was found with any of the investigated SNPs, including the previously reported 5A/6A polymorphism (rs3025058), with regard to TVR using single SNP analysis or haplotype analysis.Conclusion: We found no significant association of MMP2 or MMP3 with TVR with this SNP-broad gene approach. Although we did not test all the known polymorphisms of these genes, using tagging analyses we examined those SNPs covering all known haplotypes of MMP2 and MMP3 to conclude that these genes do not correlate with a genetic risk of coronary restenosis after successful PCI.
Expression Levels of Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) in Primary Breast Carcinoma and Distant Breast Cancer Metastases
Authors: Ihnen M. , Köhler N. , Kersten J. F. , Milde-Langosch K. , Beck K. , Höller S. , Müller V. , Witzel I. , Jänicke F. , Kilic E. 
Published: June 2013

Introduction:Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) gained increasing attention regarding tumorprogression and metastatic spread in breast cancer. The aim of this study was to examine ALCAM expression levels in primary breast cancer and distant metastases of the same patient within 29 autopsy cases to better understand the underlying mechanisms of metastases and the role of adhesion molecules in this process.Material and Methods:Paraffin-embedded tissue of the primary and distant metastases (N = 84) were collected and ALCAM immunohistochemistry was performed.Results:The primary tumor and all metastases showed a statistically normally distributed ALCAM expression. ALCAM expression level average differs between immunoreactive score (IRS) (mean) 4.16 (lung)-5.00 (adrenal gland). Of the metastatic ALCAM expression levels we obtained an intra-class correlation (ICC) of 80.9%, indicating a strong cluster effect of measurements in the same patient. ALCAM expression scores in metastatic sites and in the primary analyzed by hierarchical regression analysis showed that ALCAM expression in the primary is prognostic for ALCAM expression in all different sites of metastases (slope = 0.773,p< 0.001,r2= 0.504).Conclusion:ALCAM expression in the primary is positively correlated to ALCAM expression in metastases within one single patient. This could show a tumorbiological context of ALCAM for the development of metastases in breast cancer.
MicroRNAs, Innate Immunity and Ventricular Rupture in Human Myocardial Infarction
Authors: Zidar Nina , Boštjančič Emanuela , Glavač Damjan , Štajer Dušan 
Published: June 2013

MicroRNAs are non-coding RNAs, functionioning as post-transcriptional regulators of gene expression. Some microRNAs have been demonstrated to play a role in regulation of innate immunity. After myocardial infarction (MI), innate immunity is activated leading to an acute inflammatory reaction. There is evidence that an intense inflammatory reaction might contribute to the development of ventricular rupture (VR) after MI.Using real-time PCR, we analysed the expression ofmiR-146a, miR-150, andmiR-155in autopsy samples of infarcted heart tissue from 50 patients with MI (23 with VR and 27 without VR).An altered expression of all three microRNAs was found in MI compared to the normal hearts. Comparing MI patients with VR and those without VR, we foundmiR-146aup-regulation, andmiR-150andmiR-155down-regulation in patients with VR.In conclusion, our study demonstrated an altered expression ofmiR-146a, miR-150, andmiR-155in MI compared to the normal hearts. These microRNAs are involved in regulation of the innate immunity. Differential expression of these microRNAs in MI patients with VR in comparison to those without VR provides further evidence that innate immunity resulting in an intense inflammatory reaction plays an important role in the pathogenesis of VR after MI in humans.
Putative Roles of Circulating Resistin in Patients with Asthma, COPD and Cigarette Smokers
Authors: Al Mutairi Sana S. , Mojiminiyi Olusegun A. , Shihab-Eldeen Aida , Al Rammah Tahani , Abdella Nabila 
Published: June 2013

Aims:To investigate the hypothesis that circulating resistin reflects the degree of pulmonary inflammation, this study explores putative roles of resistin in patients with acute and stable inflammatory obstructive airway diseases and cigarette smokers.Methods:We determined complements C3, C4, fasting resistin, insulin, glucose and lipid profile; calculated insulin resistance (homeostasis model assessment (HOMA-IR) in patients with acute asthma exacerbation (n= 34); stable asthma (n= 26) and stable chronic obstructive pulmonary disease (COPD;n= 26), cigarette smokers (n= 81), and healthy control subjects (n= 42). We determined the associations between these variables and pulmonary function tests.Results: Patients with COPD, acute and stable asthma had significantly higher resistin and insulin than control subjects. Resistin, insulin, HOMA-IR, FEV1% and FEV1/FVC were significantly (p< 0.05) different between patients with acute asthma compared with stable asthma and COPD; smokers had similar levels of resistin, C3 and C4 as patients with asthma and COPD. In smokers, patientswith asthma or COPD, resistin showed significant inverse correlations with FEV1%; FEV1/FVC% and positive significant correlations with BMI and HOMA-IR. Logistic regression showed that resistin is associated (p< 0.05) with inflammatory obstructive airways disease − odds ratio (OR) = 1.22 and smoking OR = 1.18.Conclusion: Resistin may be a disease activity marker and may contribute to insulin resistance in smokers, asthma and COPD.
Diagnostic and Prognostic Molecular Markers in Hepatocellular Carcinoma
Authors: Mínguez Beatriz , Lachenmayer Anja 
Published: June 2013

Hepatocellular carcinoma (HCC) is one of the most lethal cancers worldwide, representing also the main cause of death among cirrhotic patients. In contrast to most other solid tumors, the underlying cirrhotic liver disease in HCC patients greatly impairs tumor related prognosis, conferring this neoplasm a unique situation, in which accurate prognostic prediction is a relevant and unmet need.Although clinical staging systems have improved significantly and now comprise tumor characteristics, liver function and patient performance status, the integration of molecular data into these algorithms is still hypothetical.Molecular profiling of HCC has led to a better understanding of the physiopathology of this neoplasm and has allowed developing novel therapeutic approaches (e.g. molecular targeted therapies) for a tumor previously considered as therapy-refractory. Integrative analysis of different reported genomic datasets has revealed common subclasses between different studies, highlighting their biological relevance in HCC. Gene signatures derived from tumors and from the adjacent tissue have been able to differentiate subclasses with different outcomes and have been proposed as potential predictive markers in the clinical setting. Genomic characterization of surrounding non-tumor tissue might be of particular interest to identify patients at high risk of developing HCC and therefore to select those patients that would benefit of potential chemopreventive strategies.Epigenetic analyses (methylation and miRNA profiling) are adding up to the knowlegde derived from gene expression data and should not be forgotten in the molecular diagnosis of HCC. Integrative analyses of genetic and epigenetic information of the tumor and the surrounding tissue should be used to identify novel biomarkers and therapeutic targets in HCC, to improve existing treatment algorithms and to eventually design a more personalized medicine in this devastating disease.
Opposite Effects ofGSTM1– andGSTT1– Gene Deletion Variants on Bone Mineral Density
Authors: Mlakar Simona Jurkovic , Osredkar Josko , Prezelj Janez , Marc Janja 
Published: June 2013

Oxidative stress is associated with osteoporosis. The glutathione S-transferases form the major detoxifying group of enzymes responsible for eliminating products of oxidative stress. We have therefore proposedGSTM1andGSTT1genes as candidates for studying the genetics of osteoporosis.The aim of the present study was to examine possible association ofGSTM1andGSTT1gene deletion polymorphisms, alone or in combination, with bone mineral density at femoral neck (BMD_fn), lumbar spine (BMD_ls) and total hip (BMD_th) in Slovenian elderly women and men.GSTM1andGSTT1gene deletion polymorphisms in 712 elderly people were analyzed using the triplex PCR method for the presence ofGSTM1andGSTT1gene segments. BMD_fn, BMD_ls and BMD_th were measured by the dual-energy X-ray absorptiometry (DEXA) method. Results were analyzed using univariate statistic model adjusted for sex, body mass index (BMI) and age.Our results showed the significant differences in BMD_th, BMD_ls and BMD_fn values (p= 0.031, 0.017 and 0.023, respectively) in subgroups ofGSTT1gene deletion polymorphism. ForGSTM1gene deletion polymorphism borderline significant association was found with BMD_ls (p= 0.100). Furthermore, subjects with homozygous deletion ofGSTT1gene showed higher BMD values on all measured skeletal sites and, in contrast, subjects with homozygous deletion ofGSTM1gene showed lower BMD values. Moreover, a gene-gene interaction study showed significant association ofGSTM1-null andGSTT1-null polymorphisms with BMD_ls values (p= 0.044). Carriers with a combination of the presence ofGSTT1gene and the homozygous absence ofGSTM1gene fragment were associated with the lower BMD values at all skeletal sites.The significant association of combination ofGSTT1gene presence and homozygous absence ofGSTM1gene with BMD was demonstrated, suggesting that it could be used, if validated in other studies, as genetic marker for low BMD.
Increased Salivary Levels of 8-Hydroxydeoxyguanosine May Be a Marker for Disease Activity for Periodontitis
Authors: Sezer Ufuk , Çiçek Yasin , Çanakçi Cenk Fatih 
Published: June 2013

Background: 8-hydroxydeoxyguanosine (8-OHdG) is commonly used as a marker to evaluate oxidative DNA damage in disorders including chronic inflammatory diseases such as inflammatory periodontal pathologies. In the current study we hypothesized that the level of 8-OHdG in saliva increases by the periodontal destruction severity determined by clinical parameters as clinical attachment level (CAL).Materials and methods: A cross-sectional study was conducted on a sum of 60 age gender balanced; chronic periodontitis (CP) (n= 20), chronic gingivitis (CG) (n= 20) and healthy (H) (n= 20) individuals. Clinical periodontal parameters and salivary 8-OHdG levels were evaluated.Results: The mean 8-OHdG level in the saliva of the CP group was significantly higher than H and CG groups (p< 0.001). Statistically significant correlation was only observed between the salivary levels of 8-OHdG and age (p< 0.05), probing depth (PD) and CAL (p< 0.001) in CP group. However, when CP patients were classified according to their CAL levels (CAL≥ 3 mm (n= 11) and CAL<3 mm (n= 9)) statistically significant correlation was only observed between the salivary levels of 8-OHdG and CAL≥ 3 mm patients (p< 0.001).Conclusion: We suggest that elevated salivary levels of 8-OHdG may be a marker for disease activity and it may reflect indirectly disease severity parameters such as CAL.
Salivary Markers of Oxidative Stress and Their Relation to Periodontal and Dental Status in Children
Authors: Tóthová L’ubomíra , Celecová Viera , Celec Peter 
Published: June 2013

Background: Previous studies have shown that salivary thiobarbituric acid reactive substances are related to the periodontal status in adults. Such an analysis has not been done on children yet. The aim of our study was to analyze salivary markers of oxidative stress in relation to periodontal and dental status in children.Methods: The periodontal and dental status of 82 consecutive pediatric dental patients was assessed. The oral hygiene index (OHI), the papillary bleeding index (PBI) and the caries index (CI) were assessed as clinical parameters. Markers of oxidative stress and antioxidant status were measured in whole saliva samples.Results: Multivariate analysis of covariance showed that the variability of PBI explains 10.9% of the variance of salivary thiobarbituric acid reacting substances (TBARS). Advanced oxidation protein products (AOPP) were related to CI (eta 8.6%). Measures of antioxidant status (total antioxidant capacity and ferric reducing ability of saliva) were partially determined by OHI (13.6% and 7.2%) and PBI (16.9% and 7.9%).Conclusions: Antioxidant status in saliva is related to oral hygiene and periodontal status. Salivary TBARS are a potential sensitive marker of periodontitis in children, similarly to adults, at least on a population level. Salivary AOPP are related to caries. Potential diagnostic value of the analyzed markers should be analyzed in further interventional studies.