bluuebunny/crossref_metadata_2025_split huggingface dataset:
ADAM10 mediates N‐cadherin ectodomain shedding during retinal ganglion cell differentiation in primary cultured retinal cells from the developing chick retina
Authors: Paudel Sharada , Kim Yeoun‐Hee , Huh Man‐Il , Kim Song‐Ja , Chang Yongmin , Park Young Jeung , Lee Kyoo Won , Jung Jae‐Chang 
Published: November 2012

AbstractHere, we examined the role of ADAM10 during retinal cell differentiation in retinal sections and in vitro cultures of developing chick retinal cells from embryonic day 6 (ED6). Immunohistochemistry showed that ADAM10 is abundantly expressed in the inner zone of neuroblastic layer at ED5, and it becomes more highly expressed in the ganglion cell layer at ED7 and ED9. Western blotting confirmed that ADAM10 was expressed as an inactive pro‐form that was processed to a shorter, active form in control cultured cells, but in cultures treated with an ADAM10 inhibitor (GI254023X) and ADAM10‐specific siRNA, the level of mature ADAM10 decreased. Phase‐contrast microscopy showed that long neurite extensions were present in untreated cultures 24 h after plating, whereas cultures treated with GI254023X showed significant decreases in neurite extension. Immunofluorescence staining revealed that there were far fewer differentiated ganglion cells in ADAM10 siRNA and GI254023X‐treated cultures compared to controls, whereas the photoreceptor cells were unaltered. The Pax6 protein was more strongly detected in the differentiated ganglion cells of control cultures compared to ADAM10 siRNA and GI254023X‐treated cultures. N‐cadherin ectodomain shedding was apparent in control cultures after 24 h, when ganglion cell differentiation was observed, but ADAM10 siRNA and GI254023X treatment inhibited these processes. In contrast, N‐cadherin staining was strongly detected in photoreceptor cells regardless of ADAM10 siRNA and GI254023X treatment. Taken together, these data indicate that the inhibition of ADAM10 can inhibit Pax6 expression and N‐cadherin ectodomain shedding in retinal cells, possibly affecting neurite outgrowth and ganglion cell differentiation. J. Cell. Biochem. 114: 942–954, 2013. © 2013 Wiley Periodicals, Inc.
Stabilization of the prostate‐specific tumor suppressor NKX3.1 by the oncogenic protein kinase Pim‐1 in prostate cancer cells
Authors: Padmanabhan Achuth , Gosc Eliza B. , Bieberich Charles J. 
Published: November 2012

AbstractLoss of NKX3.1 is an early and consistent event in prostate cancer and is associated with increased proliferation of prostate epithelial cells and poor prognosis. NKX3.1 stability is regulated post‐translationally through phosphorylation at multiple sites by several protein kinases. Here, we report the paradoxical stabilization of the prostate‐specific tumor suppressor NKX3.1 by the oncogenic protein kinase Pim‐1 in prostate cancer cells. Pharmacologic Pim‐1 inhibition using the small molecule inhibitor CX‐6258 decreased steady state levels and half‐life of NKX3.1 protein but mRNA was not affected. This effect was reversed by inhibition of the 26S‐proteasome, demonstrating that Pim‐1 protects NKX3.1 from proteasome‐mediated degradation. Mass spectrometric analyses revealed Thr89, Ser185, Ser186, Ser195, and Ser196 as Pim‐1 phospho‐acceptor sites on NKX3.1. Through mutational analysis, we determined that NKX3.1 phosphorylation at Ser185, Ser186, and within the N‐terminal PEST domain is essential for Pim‐1‐mediated stabilization. Further, we also identified Lys182 as a critical residue for NKX3.1 stabilization by Pim‐1. Pim‐1‐mediated NKX3.1 stabilization may be important in maintaining normal cellular homeostasis in normal prostate epithelial cells, and may maintain basal NKX3.1 protein levels in prostate cancer cells. J. Cell. Biochem. 114: 1050–1057, 2013. © 2012 Wiley Periodicals, Inc.
Regulation of human Cripto‐1 expression by nuclear receptors and DNA promoter methylation in human embryonal and breast cancer cells
Authors: Bianco Caterina , Castro Nadia P. , Baraty Christina , Rollman Kelly , Held Natalie , Rangel Maria Cristina , Karasawa Hideaki , Gonzales Monica , Strizzi Luigi , Salomon David S. 
Published: November 2012

AbstractHuman Cripto‐1 (CR‐1) plays an important role in regulating embryonic development while also regulating various stages of tumor progression. However, mechanisms that regulate CR‐1 expression during embryogenesis and tumorigenesis are still not well defined. In the present study, we investigated the effects of two nuclear receptors, liver receptor homolog (LRH)‐1 and germ cell nuclear factor receptor (GCNF) and epigenetic modifications on CR‐1 gene expression in NTERA‐2 human embryonal carcinoma cells and in breast cancer cells. CR‐1 expression in NTERA‐2 cells was positively regulated by LRH‐1 through direct binding to a DR0 element within the CR‐1 promoter, while GCNF strongly suppressed CR‐1 expression in these cells. In addition, the CR‐1 promoter was unmethylated in NTERA‐2 cells, while T47D, ZR75‐1, and MCF7 breast cancer cells showed high levels of CR‐1 promoter methylation and low CR‐1 mRNA and protein expression. Treatment of breast cancer cells with a demethylating agent and histone deacetylase inhibitors reduced methylation of the CR‐1 promoter and reactivated CR‐1 mRNA and protein expression in these cells, promoting migration and invasion of breast cancer cells. Analysis of a breast cancer tissue array revealed that CR‐1 was highly expressed in the majority of human breast tumors, suggesting that CR‐1 expression in breast cancer cell lines might not be representative of in vivo expression. Collectively, these findings offer some insight into the transcriptional regulation of CR‐1 gene expression and its critical role in the pathogenesis of human cancer. J. Cell. Physiol. 228: 1174–1188, 2013. © 2012 Wiley Periodicals, Inc.
Codon 72 polymorphism of p53 and HPV type 16 E6 variants as risk factors for patients with squamous epithelial lesion of the uterine cervix
Authors: Burroni E. , Bisanzi S. , Sani C. , Puliti D. , Carozzi F. 
Published: November 2012

AbstractThe Arg/Arg genotype versus Arg/Pro or Pro/Pro at codon 72 of the p53 gene in association with human papillomavirus (HPV) 16 E6 variants has been implicated as a risk marker in cervical neoplasia. However, research on this topic has produced controversial results. The association of p53 codon 72 polymorphism alone and in combination with specific HPV 16 E6 variants with risk of developing squamous intraepithelial cervical lesion has been investigated in low and high‐grade squamous intraepithelial lesions and in HPV‐negative controls from an Italian population. The data obtained showed statistically significant different distribution of p53 genotypes between healthy controls and precursor lesions, with the p53 arginine homozygous increased in high‐grade squamous intraepithelial lesions. The T350G HPV 16 variant was the most frequent variant observed in the analyzed group of Italian women, showing a slight decreasing with the severity of the lesion. At the same time, the number of the prototype T350 slightly increased with the severity of the cytological lesions. In conclusion, p53 arginine homozygous was found to be increased in high‐grade lesions, supporting the results of previous investigations indicating that HPV‐positive patients with p53 Arg/Arg have an increased risk of developing pre‐cancerous lesions. In addition, T350G HPV 16 variant was over‐represented in p53 Arg homozygous women with cervical lesions. When p53 genotype and HPV 16 variants are considered together, no difference emerges between cases and controls so is not possible to assess that the oncogenic effect of HPV 16 T350G variant may be influenced by the p53 genotype. J. Med. Virol. 85:83–90, 2012. © 2012 Wiley Periodicals, Inc.
Polygonum viviparum L. inhibits the lipopolysaccharide‐induced inflammatory response in RAW264.7 macrophages through haem oxygenase‐1 induction and activation of the Nrf2 pathway
Authors: Cheng Hui‐Wen , Lee Kock‐Chee , Cheah Khoot‐Peng , Chang Ming‐Long , Lin Che‐Wei , Li Joe‐Sharg , Yu Wen‐Yu , Liu E‐Tung , Hu Chien‐Ming 
Published: November 2012

AbstractBACKGROUND: Polygonum viviparum L. (PV) is a member of the family Polygonaceae and is widely distributed in high‐elevation areas. It is used as a folk remedy to treat inflammation‐related diseases. This study was focused on the anti‐inflammatory response of PV against lipopolysaccharide (LPS)‐induced inflammation in RAW264.7 macrophages.RESULTS: Treatment with PV did not cause cytotoxicity at 0–50 µg mL−1 in RAW264.7 macrophages, and the IC50 value was 270 µg mL−1. PV inhibited LPS‐stimulated nitric oxide (NO), prostaglandin (PG)E2, interleukin (IL)‐1β and tumour necrosis factor (TNF)‐α release and inducible NO synthase (iNOS) and cyclooxygenase (COX)‐2 protein expression. In addition, PV suppressed the LPS‐induced p65 expression of nuclear factor (NF)‐κB, which is associated with the inhibition of IκB‐α degradation. These results suggest that, among mechanisms of the anti‐inflammatory response, PV inhibits the production of NO and these cytokines by down‐regulating iNOS and COX‐2 gene expression. Furthermore, PV can induce haem oxygenase (HO)‐1 protein expression through nuclear factor E2‐related factor 2 (Nrf2) activation. A specific inhibitor of HO‐1, zinc(II) protoporphyrin IX, inhibited the suppression of iNOS and COX‐2 expression by PV.CONCLUSION: These results suggest that PV possesses anti‐inflammatory actions in macrophages and works through a novel mechanism involving Nrf2 actions and HO‐1. Thus PV could be considered for application as a potential therapeutic approach for inflammation‐associated disorders. © 2012 Society of Chemical Industry
Stress‐Induced Stabilization of Crystals in Shape Memory Natural Rubber
Authors: Heuwers Benjamin , Quitmann Dominik , Hoeher Robin , Reinders Frauke M. , Tiemeyer Sebastian , Sternemann Christian , Tolan Metin , Katzenberg Frank , Tiller Joerg C. 
Published: November 2012

AbstractIn contrast to all known shape memory polymers, the melting temperature of crystals in shape memory natural rubber (SMNR) can be greatly manipulated by the application of external mechanical stress. As shown previously, stress perpendicular to the prior programming direction decreases the melting temperature by up to 40 K. In this study, we investigated the influence of mechanical stress parallel to prior stretching direction during programming on the stability of the elongation‐stabilizing crystals. It was found that parallel stress stabilizes the crystals, which is indicated by linear increase of the trigger temperature by up to 17 K. The crystal melting temperature can be increased up to 126.5 °C under constrained conditions as shown by X‐ray diffraction measurements.
Perceptions of Issues Relating to Exercise and Joint Health in Rheumatoid Arthritis: A UK‐Based Questionnaire Study
Authors: Law Rebecca‐Jane , Markland David A. , Jones Jeremy G. , Maddison Peter J. , Thom Jeanette M. 
Published: November 2012

AbstractObjectivesThis questionnaire study investigated the perceptions of rheumatoid arthritis (RA) patients across the UK in relation to exercise and joint health. The validity of the measure was also assessed.MethodsMembers of the National Rheumatoid Arthritis Society (NRAS) with self‐reported RA completed the questionnaire online. Items related to five factors that emerged from previous qualitative research. Participants responded using a five‐point Likert‐style scale (strongly disagree to strongly agree). The International Physical Activity Questionnaire (IPAQ) assessed physical activity. The model was tested using confirmatory factor analysis (LISREL 8.8); statistical analyses were conducted using the Statistical Package for the Social Sciences (SPSS).ResultsA total of 247 responses were collected over 47 days (88% females; age: 18–77 years; disease duration: <1–51 years). Acceptable factorial validity was revealed (Satorra–Bentler χ2 = 774.47, df = 454, p < 0.001, root mean squared error of approximation (RMSEA) = 0.05, 90% confidence interval RMSEA = 0.05–0.06, comparative fit index = 0.94, standardized root mean square residual = 0.09), with the following factor endorsements: ‘Health professionals show exercise knowledge’ (19%); ‘Knowing what exercise should be done’ (43%); ‘Having to exercise because it is helpful’ (72%); ‘Worry about causing harm to joints’ (44%); and ‘Not wanting to exercise as joints hurt’ (52%). Patient concerns about joint pain, joint harm and how to exercise were significantly associated with lower physical activity (p < 0.05).ConclusionsThese results confirm that patients perceive exercise as beneficial. However, concerns about how to exercise, joint pain, causing harm to joints and a perceived lack of exercise knowledge among health professionals remain. Addressing these concerns may have implications for increasing physical activity within the RA population. Copyright © 2012 John Wiley & Sons, Ltd.
Adherence to ICCS nomenclature guidelines in subsequent literature: A bibliometric study
Authors: Dannaway Jasan , Ng Heryanto , Deshpande Aniruddh V 
Published: November 2012

AbstractAimsSince the publication of the 2006 International Children's Continence Society (ICCS) guidelines on terminologies for lower urinary tract dysfunction in children, little is known of their impact. In this study, we aim to quantify the adherence to the guidelines in the published literature, and to examine whether Medical Subject Headings (MeSH) in MEDLINE reflect the recommended “new” ICCS terminology.MethodsSeven pairs of pre‐specified paired terms (obsolete and recommended by the ICCS) were searched, limited to paediatric literature published between 2002 and 2010. Their use in the literature was compared between the pre‐guideline (2002–2005) and post‐guideline (2007–2010) period and across geographical regions. MeSH in MEDLINE were examined for the use of ICCS preferred terminology.ResultsPublications in paediatric urinary incontinence have shown a 49% increase from 2002–2005 to 2007–2010 (55–82 per year). There was about a fourfold increase in the likelihood of usage of ICCS recommended terminologies post ICCS guideline publication (OR: 4.19, 95% CI: 3.04–5.78, P < 0.001). Approximately 25% of the studies published between 2007 and 2010 used obsolete terminologies. Analysis indicated satisfactory uptake for most terms, with the exception of “urotherapy.” There was no significant geographical variation in uptake. More than half of the ICCS‐recommended terms (4/7) did not appear in the current MeSH indexing tree and scope notes.ConclusionsOverall uptake of recommended terms following release of ICCS terminology guidelines was encouraging although it remains suboptimal for certain terms. Efforts need to be made to improve the current MEDLINE indexing so that MeSH terms reflect terminology recommended by the ICCS. Neurourol. Urodynam. 32: 952–956, 2013. © 2012 Wiley Periodicals, Inc.
The association between socioeconomic status and survival among children with Hodgkin and non‐Hodgkin lymphomas in a universal health care system
Authors: Darmawikarta Denise , Pole Jason D. , Gupta Sumit , Nathan Paul C. , Greenberg Mark 
Published: November 2012

AbstractBackgroundThe association between socioeconomic status (SES) and cancer survival has been studied extensively in adults. However, little is known about this relationship in the pediatric population, specifically in jurisdictions with universal health care insurance programs. Our aim was to determine whether lower SES is associated with poorer survival in pediatric Hodgkin (HL) and non‐Hodgkin lymphoma (NHL) patients in Ontario.ProcedureAll incident cases of HL and NHL in children between 0 and 14 years old diagnosed in Ontario between January 1st, 1985 and December 31st, 2006 were identified through the Pediatric Oncology Group of Ontario Networked Information System. Neighborhood income quintile and material deprivation quintile at diagnosis were used as proxies for SES. Cox proportional hazards regressions were used to assess the association between SES and the risk of event‐free or overall survival.ResultsA total of 692 patients were included in the analysis: 302 HL and 390 NHL. SES was not associated with survival (overall or event‐free) among HL and NHL patients (P > 0.05 for all four comparisons, i.e., HL/NHL, EFS/OS) after adjustment for age, sex, period of diagnosis, and disease stage. There were no differences in the distribution of disease stage across SES strata at the time of diagnosis. Similarly, the distribution of deaths among long‐term survivors (survived ≥5 years from diagnosis) did not differ across SES strata (P > 0.05).ConclusionsSES was not associated with risk of death among pediatric HL and NHL patients in Ontario. This was consistent through the cancer trajectory, including diagnosis, treatment, and survivorship. Pediatr Blood Cancer 2013; 60: 1171–1177. © 2012 Wiley Periodicals, Inc.
Risk of adverse events following oseltamivir treatment in influenza outpatients, Vaccine Safety Datalink Project, 2007–2010
Authors: Greene Sharon K. , Li Lingling , Shay David K. , Fry Alicia M. , Lee Grace M. , Jacobsen Steven J. , Baxter Roger , Irving Stephanie A. , Jackson Michael L. , Naleway Allison L. , Nordin James D. , Narwaney Komal J. , Lieu Tracy A. 
Published: November 2012

ABSTRACTPurposeAn association between the influenza antiviral medication oseltamivir and neuropsychiatric events has been suggested by post‐marketing case reports in Japan. This possible association was not supported by cohort studies in the U.S. conducted prior to the 2009 influenza A (H1N1) pandemic, when usage rates were comparatively low. We assessed oseltamivir safety before and during the pandemic using biologically plausible risk intervals, particularly focusing on psychiatric events.MethodsOutpatients with influenza episodes from January 2007 through June 2010 were identified using diagnosis codes and positive tests at eight health care systems (sites) in the Vaccine Safety Datalink Project. Oseltamivir‐treated and untreated patients were matched according to calendar week, age, sex, site, and propensity for treatment. Within this matched cohort, conditional logistic regression models were used to estimate the risk of four neuropsychiatric and five other adverse events (AEs) during pre‐specified risk intervals.ResultsAmong 27 684 matched pairs, no associations were identified between oseltamivir treatment and any pre‐defined AE. The absolute risks of incident psychiatric events in the 1–7 day risk interval were 0.126% for oseltamivir‐treated and 0.105% for untreated patients (odds ratio = 1.21, 95% confidence interval [CI]: 0.74, 1.97; risk difference = 0.022%, 95% CI: −0.035%, 0.078%); the most common diagnosis was unspecified anxiety state. Results were similar for 1–14 and 1–2 day risk intervals and for pediatric/adolescent subgroups.ConclusionsConsistent with prior U.S. cohort studies, no evidence was identified for an increased risk of neuropsychiatric or other AEs following oseltamivir treatment. Safety should be prospectively monitored to inform antiviral medication usage recommendations. Copyright © 2012 John Wiley & Sons, Ltd.
A questionnaire to document self‐medication history in adult patients visiting emergency departments
Authors: Roulet Lucien , Asseray Nathalie , Foucher Nadine , Potel Gilles , Lapeyre‐Mestre Maryse , Ballereau Françoise 
Published: November 2012

ABSTRACTPurposeTo develop the first questionnaire to obtain a complete medication history by documenting self‐medication history in adult patients admitted to a medical emergency department (ED).MethodsA Questionnaire to document Self‐Medicating Behaviours (QSMB) was developed between January and September 2008 (reference period), tested and refined between October and December 2008, and used routinely between January and December 2009 (routine period) in a tertiary care medical ED. The rate of SMBs measured with QSMB during the routine period was compared to the SMB rate measured with a spontaneous reporting method during the reference period. As survey teams changed every trimester, we also analysed the evolution of SMB rate over time.ResultsQSMB is divided into two parts. The first part consists of 20 closed‐ended questions exploring all indications and dimensions of self‐medication. The second part assesses the characteristics of each medication mentioned by the patient in the first 20 questions. The patients interviewed during reference and routine periods did not significantly differ. The routine period patients reported a third more SMBs (89.8% vs 57.6%, respectively; p < 0.0001) and twice more self‐medication drugs than the reference period patients. SMB rate was significantly different between the survey teams during the reference period (p < 0.0001), but not during the routine period (p = 0.078).ConclusionsThis questionnaire complements the traditional tools that are already available to collect medication histories of prescribed drugs. It may improve the recognition of iatrogenic conditions related to self‐medication, and provide support to public health efforts and research programs on self‐medication. Copyright © 2012 John Wiley & Sons, Ltd.
Nonlinear phase‐separation behavior of poly(methyl methacrylate)/poly(styrene‐co‐maleic anhydride) blends
Authors: Lin Yu , Shangguan Yonggang , Chen Feng , Zuo Min , Zheng Qiang 
Published: November 2012

AbstractThe nonlinear phase‐separation behavior of poly(methyl methacrylate)/poly(styrene‐co‐maleic anhydride) (PMMA/SMA) blends over wide appropriate temperature and heating rate ranges was studied using time‐resolved small‐angle laser light scattering. During the non‐isothermal process, a quantitative logarithm function was established to describe the relationship between cloud point (Tc) and heating rate (k) as given by Tc = Alnk + T0, in which the parameter A, reflecting the heating rate dependence, is much different for different compositions due to phase‐separation rate and activation energy difference. For the isothermal phase‐separation process, an Arrhenius‐like equation was successfully applied to describe the temperature dependence of the apparent diffusion coefficient (Dapp) and the relaxation time (τ) of the early stage as well as the late stage of spinodal decomposition (SD) of PMMA/SMA blends. Based on the successful application of the Arrhenius‐like equation, the related activation energies could be obtained from Dapp and τ of the early and late stages of SD, respectively. In addition, these results indicate that it is possible to predict the temperature dependence of the phase‐separation behavior of binary polymer mixtures during isothermal annealing over a range of 100 °C above the glass transition temperature using the Arrhenius‐like equation. © 2012 Society of Chemical Industry