bluuebunny/crossref_metadata_2025_split huggingface dataset:
The skin microbiome on healthy and inflammatory altered canine skin determined by next generation sequencing
Authors: Štempelová Lucia , Micenková Lenka , Andrla Petr , Strompfová Viola 
Published: February 2025

IntroductionHuman and animal skin is colonized by a complex microbial population. An imbalance of these microorganisms is often associated with dermatological diseases.MethodsThe aim of this work was to describe the skin bacterial microbiota composition of healthy dogs and dogs with inflammatory skin lesions. Genomic DNA was sequenced using primers that target the V4 region of the bacterial 16S rRNA gene. Superficial skin swabs were collected from eight body areas of six healthy dogs (n = 48) and directly from inflammatory altered canine skin (n = 16).ResultsThe skin of healthy dogs was predominantly colonized by phylum Bacillota (34.4 ± 27.2%), followed by Actinomycetota (32.2 ± 20.3%), Pseudomonadota (16.4 ± 12.2%), and Bacteroidota (8.7 ± 11.6%). At the level of genera, Streptococcus spp. (19.4 ± 26.1%) was the most abundant genus across all samples collected from healthy skin, followed by Curtobacterium (5.4 ± 12.1%), Bacteroides (5.2 ± 11.1%) and Corynebacterium_1 (4.3 ± 13.2%). More specifically, Streptococcus spp. was the most abundant on the chin (49.0 ± 35.5%), nose (37.9 ± 32.1%), perianal region (21.1 ± 28.2%), abdomen (11.0 ± 12.8%), dorsal back (12.4 ± 10.3%) and interdigital area (5.5 ± 2.2%). Curtobacterium spp. was predominant on inner pinna (17.8 ± 24.8%) and axilla (6.7 ± 10.8%). Alpha diversity analysis (Shannon index) showed maximum on interdigital area but minimum on a chin (p-value: 0.0416). Beta diversity analysis showed clustering across samples from the individual skin sites but also across samples collected from individual dogs. Staphylococcus spp. was the most abundant genus in 12/16 samples collected from inflammatory skin. In addition, a lower bacterial diversity was observed in samples from skin lesions compared to samples from healthy canine skin.DiscussionThe results confirm the fact that the microbiome of healthy skin is very diverse. Compared to other studies, streptococci predominated on healthy canine skin. Shannon index showed only minor differences in diversity between different parts of canine skin. Results of beta-diversity showed the fact that the main force driving the skin microbiota composition is the individual, followed by the skin site. On the area of skin lesions, dysbiosis was observed with a significant predominance of staphylococci.
Rhythmic auditory cues improve gait asymmetry during unobstructed walking in people with Parkinson’s disease but have no effect on obstacle avoidance - AsymmGait-Parkinson study
Authors: Cursiol Jônatas Augusto , dos Santos Paulo Cezar Rocha , Beretta Victor Spiandor , Orcioli-Silva Diego , Simieli Lucas , Schlenstedt Christian , Coelho Daniel Boari , Barbieri Fabio Augusto 
Published: February 2025

IntroductionThis study investigated the influence of rhythmic auditory cues (RAC) on gait asymmetry (GA) during unobstructed and obstacle avoidance walking in people with Parkinson’s disease (PD) and neurologically healthy individuals.MethodsThirteen individuals with PD (70.33 ± 6.02 years) and 13 healthy controls (CG) (70.77 ± 7.56 years) participated in this study. They performed a total of five trials during unobstructed walking and 10 trials during obstacle walking under each auditory cue condition (without and with RAC). For obstacle walking, five trials were performed with each limb as leading during obstacle avoidance. First, the volunteers performed the trials without RAC. The trial order, unobstructed or obstacle walking, was randomly defined, and the cues (controlled by a metronome) were personalized according to participants’ cadence. The symmetric index of gait parameters was analyzed using 2 × 2 factorial analysis of variance (group and RAC as factors) separately for each gait type (unobstructed and obstructed walking).ResultsA group-by-auditory cue interaction for step velocity (p = 0.027) showed that the PD group exhibited 57.6% reduced asymmetry with RAC during unobstructed walking, with no significant effects observed for the CG. However, RAC had no effect on GA during obstacle avoidance walking in people with PD. Conversely, the CG exhibited 10.5% greater step length asymmetry, 7.1% greater step duration asymmetry, 7.0% greater step velocity asymmetry, and 10.6% greater double support duration asymmetry during obstacle avoidance with RAC (p &lt; 0.001).ConclusionWe conclude that RAC can reduce GA in people with PD during unobstructed walking, but appear to have no effect and negative effects on GA during obstacle walking in people with PD and neurologically healthy individuals, respectively.
Relevance of persistent perfusion deficits on clinical outcomes after successful endovascular treatment: a prospective serial magnetic resonance study
Authors: Valls Carbó Adrián , Palomar Alicia , Laredo Carlos , Werner Mariano , Dorado Laura , Remollo Sebastià , Munuera Josep , Puig Josep , Silva Yolanda , Pérez de la Ossa Natalia , Gomis Meritxell , Bustamante Alejandro , Castaño Carlos , Muñoz Lucía , Domenech Sira , Terceño Mikel , Millán Mònica , Hernández-Pérez María 
Published: February 2025

BackgroundHalf of the patients who undergo successful recanalization after endovascular treatment (EVT) experience poor clinical outcomes. Impaired microvascular reperfusion (IMR) may explain this lack of improvement, but its frequency and clinical significance remain unclear. The study aims to describe the frequency and associated factors of IMR.Materials and methodsWe conducted a study on a cohort of patients with anterior large artery occlusion, treated with EVT at a single center, who achieved mTICI ≥2C. Perfusion MRI was obtained at arrival, up to 2 h after EVT (post-EVT MRI), and on day 5. IMR was observed only on the post-EVT relative cerebral blood volume (rCBV) maps as voxels within the follow-up ischemic lesion, exhibiting a &gt; 15% asymmetry compared to a mirror homolog, in the absence of internal carotid occlusion, hemorrhagic transformation, or arterial reocclusion. Patients with an IMR volume greater than 5 mL were defined as having significant IMR. IMR was analyzed as a binary variable (presence/absence using the 5 mL cut-off) and by total and relative volume.ResultsIMR was present in 8 out of 33 patients (24.2%), with 4 out of 11 (36.4%) having mTICI 2C, and 4 out of 22 (18.2%) having mTICI 3. After adjustment for relevant variables, absolute and relative IMR volumes were associated with higher National Institutes of Health Stroke Scale (NIHSS) scores at 5 days (adjusted beta =0.50 [0.05, 0.96], p = 0.03) and at 24 h (adjusted beta = 0.11 [0.02, 0.19], p = 0.01). No independent associations were found between IMR and the 90-day modified Rankin Scale (mRS).ConclusionIMR is present in one-quarter of patients and is associated with worse early neurological outcomes.
A new nomogram for predicting 90-day outcomes of intravenous thrombolysis in patients with acute ischaemic stroke
Authors: Zhao Yingjie , Zhang Rui , Li Pan , Zhang Zhen , Yu Huan , Su Zhaoya , Xia Yandong , Meng Aiguo 
Published: February 2025

BackgroundThe aim of this study was to construct and validate a new nomogram to predict the risk of poor outcome in patients with acute ischemic stroke (AIS) after intravenous thrombolytic therapy (IVT).MethodsA total of 425 patients who received IVT within 4.5 h of stroke onset were included in a retrospective study. All the patients were divided into training (70%, n = 298) and validation cohorts (30%, n = 127). Poor outcome (defined as a 90-day modified Rankin Scale score 3–5) was the primary outcome. Logistic regression was used for analysis of independent risk factors for poor outcome in patients with AIS. Nomograms of poor outcome in AIS patients were constructed using R software. Discrimination and calibration of the models were assessed using area under the receiver operating characteristic (ROC) curve (AUC) and calibration plots.ResultsMultifactorial logistic regression analysis showed that SII (OR = 1.001, 95% CI: 1.000–1.002, p = 0.008), SIRI (OR = 1.584, 95% CI: 1.122–2.236, p = 0.009), NIHSS (OR = 1.101, 95% CI: 1.044–1.160, p &lt; 0.001), and history of diabetes mellitus (OR = 2.582, 95% CI: 1.285–5.188, p = 0.008) were the independent risk factors for the occurrence of poor outcome in AIS patients. The poor outcome nomogram for AIS patients was constructed based on the above independent risk factors. The training and validation cohort AUCs of the nomogram were 0.854 (95% CI: 0.807–0.901) and 0.855 (95% CI: 0.783–0.927), respectively. The prediction models were well calibrated in both the training and validation cohorts. The net benefit of the nomograms was better when the threshold probability ranges were 4.28–66.4% and 4.01–67.8% for the training and validation cohorts, respectively.ConclusionNew nomogram includes NIHSS, SII, SIRI and diabetes as variables with the potential to predict the risk of 90-day outcomes in patients with AIS following IVT.
Hypofractionated stereotactic radiotherapy using coplanar VMAT for single small brain metastasis: dosimetric analysis and clinical outcomes
Authors: Lai Jialu , Li An , Zeng Xianhu , Liu Jia , Zhou Lin 
Published: February 2025

PurposeTo evaluate the dosimetric parameters and clinical outcomes of hypofractionated stereotactic radiotherapy (HSRT) for small brain metastases [BMs; planning target volume (PTV) ≤ 4 cm3) via coplanar volumetric modulated arc therapy (C-VMAT).MethodsBetween March 2019 and February 2023, 68 patients with a single BM treated with Linac-based HSRT (24–39 Gy in three fractions) via C-VMAT and a 3-mm PTV margin were enrolled in this retrospective analysis. A frameless head–neck–shoulder thermoplastic mask, whose immobilization accuracy is inferior to that of specialized mask fixation systems, was used to immobilize patients. Dosimetric parameters and clinical outcomes were evaluated.ResultsC-VMAT provided clinically satisfactory treatment plans, with median gradient index, conformity index, homogeneity index, and PTV coverage values of 4.30, 1.05, 1.28, and 98%, respectively. The median volumes of normal brain tissue receiving 18 Gy, 21 Gy, and 23 Gy were 7.29 cm3, 5.33 cm3, and 4.40 cm3, respectively. High delivery accuracy was observed, with a gamma passing rate ≥90% for all plans. As of June 2023, the median follow-up time was 9.1 months. The intracranial objective response rate and disease control rate were 64% and 96%, respectively. The median intracranial progression-free survival was 26.9 (95% CI, 12.7–41.1) months. The 1- and 2-year local control (LC) rates were 91.5% (95% CI, 80.1%–100%) and 83.2% (95% CI, 64.6%–100%), respectively. The 1- and 2-year intracranial control rates were 70.9% (95% CI, 55.2%–86.6%) and 51.2% (95% CI, 32.6%–69.8%), respectively. Only four irradiated lesions progressed at the end of follow-up. The cerebral radiation necrosis rate of all patients was 7.4%.ConclusionC-VMAT HSRT combined with a 3-mm PTV margin is an effective and safe treatment modality for small BMs.
XueBiJing injection reduced mortality in sepsis patients with diabetes
Authors: Liu Yan , Dai Hengheng , Li Yixuan , Yang Tianyi , Zhang Dandan , Hu Chaoyue , Liu Si , Feng Zhiqiao , Zhang Chi , Yang Xiaohui 
Published: February 2025

IntroductionSepsis patients with diabetes are at a high clinical risk. It is well reported that XueBiJing injection has good clinical benefit in sepsis individuals. However, there is no relevant report about the efficacy and safety of XBJ in sepsis patients with comorbid diabetes.MethodsData of two large randomized controlled clinical trials (XBJ-SAP (ChiCTR-TRC-13003534) and EXIT-SEP (NCT0323874)) were combined, and post hoc analyses were performed. Sepsis patients with diabetes were further divided into the XBJ-treated group and placebo group based on inclusion and exclusion criteria. The primary (28-day mortality) and secondary outcomes (mortality in the ICU and in the post-randomization hospital, acute physiology, and chronic health evaluation II (APACHE II) score and sequential organ failure assessment (SOFA) score) were compared between the XBJ treatment and placebo groups in sepsis patients with the diabetes status at baseline. Moreover, the occurrence of adverse events (AEs) was also assessed.ResultsAt the study baseline, a total of 378 sepsis patients (227 men [60.0%] and 151 women [40.0%]; mean [SD] age, 60.3 [11.1] years) were considered to have diabetes, of which 177 received XBJ and 201 received placebo administration. Among these sepsis patients with diabetes, the mortality at 28 days was significantly lower in the XBJ group than in the placebo group (29 of 173 patients [16.8%] vs. 56 of 198 patients [28.3%], P = 0.01), and the absolute risk difference was 11.5% (95% CI, 3.1%–19.9%). Furthermore, there was no difference in the overall incidence of adverse events (AEs) when XBJ was used (24.4% [42 of 172 patients] vs. 27.7% [54 of 195 patients].DiscussionThe present study underscores the pivotal role of XBJ in modulating the immune response among sepsis patients suffering from diabetes mellitus, exploring the positive effects of XBJ on sepsis patients with diabetes mellitus. The efficacy and safety of XBJ compared with those of the placebo were consistent with the overall trial findings, demonstrating that XBJ is efficacious in sepsis patients with diabetes and suggesting that there is no need for special safety precautions.Trial Registration IdentifierChiCTR-TRC-13003534 and NCT0323874.
Parent–child attachment and adolescent problematic behavior: the mediating effect of legal emotions
Authors: Jianhua He , Su Xu , Shuhui Xu 
Published: February 2025

IntroductionIn criminology, the close relationship between legal emotions and adolescent deviant behavior is well-documented. In psychology, parental attachment is strongly associated with children’s problematic behavior; however, the role of legal emotions in this relationship remains underexamined.MethodsThis study investigates the role of legal emotions in the relationship between adolescent parent–child attachment and problematic behavior. Adolescents completed self-report questionnaires.ResultsResults revealed that both paternal and maternal attachment significantly negatively predicted adolescent problematic behavior, with paternal attachment explaining a larger proportion of the variance. Even after controlling for factors such as gender and grade level, parental attachment significantly negatively predicted adolescent problematic behavior. Legal emotions partially mediated the relationship between parental attachment and problematic behavior.ConclusionThese findings suggest that fostering positive parent–child relationships and enhancing adolescents’ positive legal emotions may be effective strategies for reducing problematic behaviors among youth.
Comparison of personality and psychopathology in patients with Fabry disease and patients with end-stage renal disease: a preliminary study
Authors: De Pasquale Concetta , Pistorio Maria Luisa , Veroux Massimiliano , Moretta Tania , Rodolico Margherita Stefania , Monte Ines Paola , Barbagallo Noemi , Zanoli Luca , Faro Denise Cristiana , Giaquinta Alessia , Maria Giambra Martina , Veroux Pierfrancesco 
Published: February 2025

BackgroundThe present study aimed to investigate personality characteristics and psychopathological symptoms in patients with Fabry disease (FD) vs a group of individuals with end-stage renal disease (ESRD).MethodsA total of 36 patients, equally divided into patients with FD and patients with ESRD (control group), were administered the following tools: the Millon Clinical Multiaxial Inventory III (MCMI-III) to evaluate personality psychopathology and the Symptom Checklist-90-R (SCL-90-R) to assess symptoms of psychopathology.ResultsSignificantly higher levels of Schizoid, Depressive, and Negativistic personality traits emerged in FD patients. Moreover, statistically significant differences in Anxiety, Interpersonal Sensitivity, Obsessive-Compulsive, Depression, Somatization, and Psychoticism dimensions of the SCL-90-R were found, with higher levels of each dimension in patients with FD than ESRD.ConclusionsThe literature, albeit limited, highlights how patients with FD are at higher risk of developing psychological distress and psychopathology than patients presenting other chronic diseases such as ESRD. Using psychological therapies together with standard treatments for FD can promote condition acceptance, reduce emotional burden, and relieve psychopathological symptoms in FD patients.
Non-suicidal self-injury in adolescence: a validation of the Chinese version of the Inventory of Statements About Self-Injury in student populations
Authors: Tian Xinhe , Huang Hebin , Lu Weicong , Zhang Ruoxi , Wang Runhua , Li Xiaoyue , Li Danping , Gao Yanling , Wu Shiyun , Xu Guiyun , Shao Robin , Lin Kangguang 
Published: February 2025

BackgroundThe Inventory of Statements About Self-Injury (ISAS) is a widely utilized scale for evaluating the 13 potential functions that motivate non-suicidal self-injury(NSSI) behaviors. However, its validation for assessing such motivation in a Chinese context is still lacking.AimsThe main objective was to access the validation of ISAS as an instrument in Chinese young population.MethodA total of 1,106 completed online self-report questionnaires were collected, with 167 reporting a history NSSI. The age range of these individuals was 12 to 24 years old, comprising 74 female and 93 male participants. The factor structure and construct validity were calculated using exploratory factor analysis (EFA) and confirmatory factor analysis (CFA). The correlations of the Patient Health Questionnaire-9 (PHQ-9), the Generalized Anxiety Disorder-7 (GAD-7), the Brief Self-Control Scale (BSCS), the Self-Rating Idea of Suicide Scale (SIOSS), and the Chinese version of the ISAS were tested using bivariate correlation analyses.ResultsThe internal consistencies of the Chinese version of the ISAS were good to excellent, with 0.788- 0.950 and 0.80-0.949 in the sports group and the junior high school group, respectively. EFA and CFA exhibited a good two-factor structure model (NFI = 0.942, CFI = 0.974, IFI = 0.974, RMSEA = 0.068, SRMR = 0.043, CMIN/DF = 1.762). Moreover, the scores of the functions of the ISAS were correlated with depression (r=0.208, p&lt;0.01), anxiety (r=0.223, p&lt;0.01), suicidal ideation (r=0.322, p&lt;0.01), and low self-control (r=-0.230, p&lt;0.01).ConclusionThis study validates the Chinese ISAS as a reliable NSSI measure, identifies a two-factor structure, and aims to inform targeted interventions and future research on self-injury behaviors among Chinese adolescents.
Malaria and missed school days: exploring school absenteeism patterns and local strategies in Odisha, India
Authors: Jabir Muhammed , Panigrahi Dilip K. , Baig Muhammad M. , Balakrishnan Vijayakumar , Panda Prasant K. , Kumar Ashwani , Rahi Manju , Shriram Ananganallur N. 
Published: February 2025

BackgroundMalaria continues to pose a significant global health challenge, disproportionately affecting children. While its effects on physical health are well-documented, the impact on education, particularly school absenteeism, remains less understood. This study aimed to explore the influence of malaria on school absenteeism in Odisha, India.MethodsA mixed-methods study was conducted in four southern districts of Odisha from September 2023 to February 2024. This involved a retrospective analysis of school attendance registers from five primary schools and qualitative interviews with 25 school teachers. Statistical analysis was performed using SATA 14.1. Mixed effects logistic regression analysis was used to determine the predictive factors of absenteeism with independent variables such as year, area of school and class. Qualitative data from interviews were transcribed and thematically analyzed.ResultsThe study evaluated absenteeism among 832 children from Class 1 to Class 5 across four academic years (2016–2020), with schools averaging 185 working days annually. While absenteeism rates varied by school, the proportion of students with yearly absenteeism rates of ≥30% remained relatively stable, ranging from 6 to 12.1%. Average absenteeism ranged from 11.5 days in the academic year 2016–2017 to a peak of 22.6 days in 2018–2019. Logistic regression analysis revealed no significant association between malaria endemicity and absenteeism patterns. Schools employed several malaria prevention and case management strategies, including health education, long-lasting insecticidal nets (LLINs), vector control, screening, testing, and on-site treatment. However, resource constraints and cultural barriers continue to pose challenges.ConclusionDespite a decline in malaria cases in the study area, school absenteeism persists due to factors beyond malaria. Future interventions should address these broader socio-cultural and logistical issues to effectively manage absenteeism and improve educational outcomes in malaria-endemic regions.
Disposal practice and determinants of unused medicines among the general public in Gondar City, Northwest Ethiopia
Authors: Yohannes Lamrot , Tegegne Addisu Afrassa , Genet Gebremariam , Workie Limenh Liknaw , Mohammed Seid Abdulwase , Alemayehu Tekletsadik Tekleslassie , Ayenew Wondim , Simegn Wudneh 
Published: February 2025

IntroductionThe inappropriate disposal of unused medicines poses significant risks to public health and the environment. Gondar City, located in Northwest Ethiopia, is not exempt from this problem. However, little is known about the current disposal practices and the determinants influencing those practices in this specific context.ObjectivesThis study aimed to evaluate the disposal practices of unused medicines among the general public in Gondar City and to identify the factors that influence these practices.MethodsA cross-sectional study design was employed to collect data from the general public in Gondar City from July 30 to August 30, 2023. Data were collected through interviews using a structured questionnaire to gather information on disposal practices and determinants that could influence disposal practices.ResultsA total of 845 respondents were involved in this study with a response rate of 93%. 56.9% of the respondents had poor disposal practices. Lower educational status [AOR = 2.76 (CI: 1.59, 4.79)]; p &lt; 0.01, having a chronic disease [AOR = 1.79 (CI: 1.22, 2.64)]; p &lt; 0.05, and poor knowledge toward disposal practices [AOR = 1.56 (CI: 1.12, 2.18)]; p &lt; 0.05 were identified as significant determinants influencing poor disposal practices.ConclusionThere is an improper disposal practice of unused medicines in the study area. Lower educational status, having a chronic disease, and poor knowledge toward disposal practices were found to be significant factors. The finding underscores the need for targeted interventions to improve the safe disposal of unused medicines in Gondar City. Comprehensive educational campaigns should be launched to increase public awareness about the risks of improper disposal and promote safe disposal practices. The establishment of accessible and convenient disposal facilities or return programs should be prioritized.