bluuebunny/crossref_metadata_2025_split huggingface dataset:
Research protocol on the impact of assessing the dynamic trajectory and the prediction of prognosis of HPV infection based on the spouse cohort of patients with oropharyngeal squamous cell carcinoma (SCOPE)
Authors: Zhu Yingying , Diao Wenwen , He Huijing , Zhu Xiaoli , Chen Xingming 
Published: August 2024

Abstract

Background: Oropharyngeal squamous cell carcinoma (OPSCC) is one of the leading cancer burdens worldwide. In China, rates of age-standardized OPSCC and mortality incidences have dramatically been increasing in recent years. Most OPSCC cases are found to be induced by human papillomavirus (HPV) through the oral-genital route during sexual activities. Dramatically increasing incidences of HPV-associated OPSCC in Chinese couples are believed to be caused by altered sexual behaviors. However, a pathophysiological understanding of the correlation between HPV infections in spouses of HPV-OPSCC patients and the impact of post-treatment HPV status on the prognosis of OPSCC is still lacking in China and requires further epidemiological evaluations and confirmatory analyses. We aim to decipher crucial etiological indicators of HPV-OPSCC by analyzing the characteristics of HPV infection in patients’ spouses through multiple cross-sectional surveys and laboratory findings for current and follow-up visits.
Methods: The spouse cohort of patients with oropharyngeal squamous cell carcinoma(SCOPE) is an open-ended bidirectional retrospective and prospective cohorts that aim to investigate the characteristics of HPV infections in spouses and their possible association with the etiological factors of HPV-OPSCC pathology through the deep analysis of long-term follow-up and laboratory data. This study proposal will adopt of more than 400 OPSCC patient-spouse pairs at Beijing Union Medical College Hospital from 2013 to date.
The primary outcome is set as overall survival (OS) and disease-specific survival (DSS), and the secondary outcome is to explore the impact of HPV infection on the reproductive health and the next generation.
Discussion: We found a correlation between HPV infection and the risk of developing OPSCC in Chinese couples. Furthermore, we compared HPV infection rates between OPSCC and non-OPSCC populations. We hypothesize that oral-genital sexual activities may play a crucial role in the pathogenesis of HPV-associated OPSCC. The absence of complete epidemiological evidence and conflicting results warrants further in-depth investigations to unveil HPV-OPSCC disease-causing factors in Chinese populations.
WITHDRAWN: No-tillage decreases GHG emissions by enhancing SOC and facilitating a stable methane-oxidizing bacterial community from an oilseed rape-rice system
Authors: Ma Mingkun , Yang Zepeng , Chen Shanghong , Liu Dinghui , Zheng Shenghua , Chen Honglin 
Published: August 2024

Abstract

The full text of this preprint has been withdrawn, as it was submitted in error. Therefore, the authors do not wish this work to be cited as a reference. Questions should be directed to the corresponding author.
Body mass estimations in extinct caviomorphs (Ctenohystrica, Hystricognathi) from cheek tooth measures: importance of predictor, reference sample and method
Authors: Boivin Myriam , Álvarez Alicia , Ercoli Marcos Darío , Moyano Rocío Silvana , Salgado-Ahumada Juan Sebastián , Tejerina Agustina Mariana Ortiz , Cassini Guillermo Hernán 
Published: August 2024

Abstract

Caviomorph rodents present an astonishing variation in sizes both nowadays and in the past. The objective of this work was to estimate the body mass of 32 extinct caviomorphs. Moreover, we seek to analyse the influence on the estimations of the: i. selected predictors (i.e., measurements on cheek teeth); ii. used reference sample comparing three distinct datasets linked to the representativeness of sizes among herbivores; and iii. employed method constructing simple and multiple regressions, which consider phylogeny and not. The results indicated that the best dental structures to predict body mass usually were the second upper and lower molars, the upper and lower rows and first upper molar. Including ungulates improved the predictions for living large rodents, while it was not the case for the living caviomorphs and small rodents. Thus, different sets of best models were proposed depending of the size range to which belongs the extinct caviomorph considered. OLS had better performance in most cases. Multiple regressions, associating dental length and width, usually improved the mean of the prediction errors with respect to simple models. Despites a probable important redundancy between these variables, independent information would be thus sufficient to improve the error of prediction. Our estimations from dental dimensions for large and giant chinchilloids are within the range of values previously obtained using a cranial variable recently considered as a robust body size proxy. For the small extinct caviomorphs, our estimations showed more deviation with respect to the body mass values available in literature.
STING Drives Pyroptosis and Exacerbates Intestinal Ischemia-Reperfusion Injury
Authors: DING Ke , Zhang Yiguo , Zhang Lele , Liao Shishi , Weng Ziyi , Chen Rong , Meng Qingtao 
Published: August 2024

Abstract

This study aimed to investigate the role of STING (Stimulator of Interferon Genes) in intestinal ischemia-reperfusion (IIR) injury, focusing on its impact on mitochondrial function, pyroptosis, and intestinal inflammation. The study utilized both in vivo and in vitro models, including a mouse model of IIR and intestinal organoids subjected to hypoxia-reoxygenation (HR). We assessed the effects of pharmacological STING inhibition using H.151 and STING knockout mouse on intestinal injury markers, including histological damage, pro-inflammatory cytokine levels, mitochondrial morphology and function, and pyroptosis markers. Our findings revealed that IIR significantly upregulated STING expression in the intestinal epithelium, coinciding with increased intestinal permeability, elevated pro-inflammatory cytokine production (IL-1β) (p < 0.05), and evidence of mitochondrial dysfunction, characterized by reduced ATP production and disrupted morphology (p < 0.01). Importantly, both H.151 treatment and STING knockout significantly attenuated IIR-induced intestinal damage (p < 0.01), reduced pro-inflammatory cytokine levels (p < 0.05), and preserved mitochondrial function (p < 0.01). Furthermore, we observed a significant reduction in pyroptosis, as evidenced by decreased cleaved gasdermin D levels, in both H.151-treated and STING knockout groups (p < 0.05). This study identifies STING as a critical mediator of IIR-induced intestinal injury, likely through the activation of pathways leading to mitochondrial dysfunction and pyroptosis. Our findings suggest that targeting STING represents a promising therapeutic strategy for mitigating intestinal damage following ischemia-reperfusion.
People without clinical diagnosis of intolerance or allergy have benefits from gluten-and casein-free diet
Authors: Penaforte Nathália de Freitas , Rodrigues Marcelo Cairrão Araújo , Guedes Arildo Lemes , de Araújo Fernando Wesley Cavalcanti , Nascimento Elizabeth do 
Published: August 2024

Abstract

Introduction and Aim: The present study aimed to assess university professors and students regarding the variation in body mass and intestinal health resulting from a restricted dietary intervention in foods containing gluten and casein. Methods: This was a non-randomized, quasi-experimental clinical trial type study. Initially, 122 volunteers aged 20 to 55 years of both sexes were evaluated, but only 63 volunteers comprised the final sample. Participants were divided into two groups: No Gluten/Casein containing Food Restriction (CG, n= 42) and Gluten/Casein containing Food Restriction (EG, for 90 days, n= 21). Approval was obtained from the Ethics Committee on Human Research at the Health Sciences Center of the Federal University of Pernambuco under protocol number 5.671.846. It also presents the Brazilian Clinical Trials Registry (ReBEC) under number RBR-6htr5rg.
Results: Scores from the Metabolic Screening Questionnaire (MSQ) in the intervention group for the thick domain showed a significant improvement compared to the NGC. Additionally, within-group comparisons showed reduced scores in the overall MSQ and domains of the digestive tract, mind, head, and emotions (p=<0.0001; p=0.0003; p=0.0434; p=0.0143; p=0.0318; respectively). Improvement in responses to the Bristol Scale was observed after gluten-and casein-free diet (GCFD). There was no reduction in body mass or Body Mass Index (BMI).
Conclusion: These results confirm our hypothesis that GCFD seems to contribute to improve intestinal function in people without diagnosed intolerances/allergies to these components. Making it a pioneer in the outlined research design.
MYC-Mediated Inhibition of ARNT2 Uncovers a Key Tumor Suppressor in Glioblastoma
Authors: Hao Yi-Heng , Borenstein-Auerbach Nofit , Grichuk Anthony , Li Li , Lafita-Navarro M. Carmen , Fang Shun , Nogueira Pedro , Kim Jiwoong , Xu Lin , Shay Jerry W. , Conacci-Sorrell Maralice 
Published: August 2024

Abstract

Tumor initiation and progression rely on intricate cellular pathways that promote proliferation while suppressing differentiation, yet the importance of pathways inhibiting differentiation in cancer remains incompletely understood. Here, we reveal a novel mechanism centered on the repression of the neuronal-specific transcription factor ARNT2 by the MYC oncogene that governs the balance between proliferation and differentiation. We found that MYC coordinates the transcriptional repression of ARNT2 through the activity of polycomb repressive complex 2 (PRC2). Notably, ARNT2, highly and specifically expressed in the central nervous system, is diminished in glioblastoma, inversely correlating with patient survival. Utilizing in vitro and in vivo models, we demonstrate that ARNT2 knockout (KO) exerts no discernible effect on the in vitro proliferation of glioblastoma cells, but significantly enhances the growth of glioblastoma cells in vivo. Conversely, ARNT2 overexpression severely dampens the growth of fully transformed glioblastoma cells subcutaneously or orthotopically xenografted in mice. Mechanistically, ARNT2 depletion diminishes differentiation and enhances stemness of glioblastoma cells. Our findings provide new insights into the complex mechanisms used by oncogenes to limit differentiation in cancer cells and define ARNT2 as a tumor suppressor in glioblastoma.
Effect of Transcranial Alternating Current Stimulation on Ataxia Symptoms of Spinocerebellar Ataxia Type 3: A Randomized Clinical Trial
Authors: Shirui Gan , Xia Liu , Wei Lin , Huating Zeng , Liangliang Qiu , Xiaoping Cheng , Wanli Zhang , Yanhua Lian , Yingjuan Zhang , Zhaodi Wang , Xichen Wang , Ruying Yuan , Xuanyu Chen , Zhixian Ye , Yue Zhang , Wang Duolao , Jun Ni , Wang Ning , Fu Ying , Chen Xin-Yuan , Qikui Sun , Shuna Huang 
Published: August 2024

Abstract

Currently, there are no treatments to alter the natural course of spinocerebellar ataxia type 3 (SCA3). Transcranial alternating current stimulation (tACS) have the sustainable potential to alleviate SCA3. Therefore, we conducted a randomized controlled trial enrolling 158 patients with SCA3 to investigate the safety and efficacy of tACS (NCT05557786). Enrolled participants received Active-tACS or Sham-tACS treatment 5 days/week for 2 weeks. The primary outcome was the proportion of participants whose Scale for the Assessment and Rating of Ataxia score improved by at least 1.5 points compared with baseline on assessments immediately after treatment and at 1-month and 3-month follow-up visits. The primary outcome assessed in the intention-to-treat population was met by 32 of 40 patients in the active-tACS group (80%) and 4 of 40 patients in the sham-tACS group (OR, 2.04[95% CI, 1.75 to 2.38; P < 0.001]). These promising findings motivate additional study to establish guidelines for tACS as a clinically recommended treatment for cerebellar diseases.
Outcomes of Vacuum Assisted Closure versus Conventional Dressings in patients with Diabetic Foot Ulcers
Authors: Ranjan Piyush , . Rishika , Mondal Saptarshi 
Published: August 2024

Abstract

Introduction: To compare the effectiveness of Vacuum Assisted Closure (VAC) dressing over Conventional Dressing in case of Diabetic Foot Ulcers. In view of this, the present study was planned to weigh between Vacuum Assisted Closure (VAC) and the conventional dressing methods in case of Diabetic Foot Ulcer in factors like rate of granulation tissue formation, Bacterial clearance in the end of the therapy and duration of wound healing. Patients and Methods: This was a randomized comparative study conducted in the department of General Surgery, Indira Gandhi Institute of Medical Sciences, Patna from January 21 to December 22.50 patients with lower limb diabetic ulcers were included who were randomized into two groups-Conventional Dressing was done(n = 31); Vacuum Assisted Closure (VAC) dressing was done(n = 19). Patients were compared for outcomes after dressing. Statistical Analysis was done by SPSS software and P value < 0.05 were considered statistically significant. Result: Compared to Conventional Dressing,Vacuum Assisted Closure (VAC) Dressing group had comparable age,gender and associated comorbidities (p value > 0.05), took less but comparable duration for granulation tissue to appear (6.94SD2.61 vs. 9.03SD2.30, p value-0.29) took significantly lesser duration for wound culture to be negative (11.36SD4.47 vs. 14.83SD3.89, p value-0.037), took significantly lesser duration for wound healing (36.84SD9.34 vs. 43.16SD9.22, p value-0.033). Conclusion: Vacuum Assisted Closure (VAC) dressing was advantageous over Conventional Dressing for Dressing of lower limb diabetic ulcers in terms of time taken for formation of granulation tissue, time taken for wound culture to be negative and on long term follow ups, also in duration of healing of wounds.Vacuum Assisted Closure (VAC) dressing for chronic wounds or ulcers seemed to be a promising option to conventional dressing and the short-term results were good.
Exploring Extracurricular Activities in Bangladesh: Access, Gender Disparities, and Shifts in Online Engagement
Authors: Jubair Hassan , Mehenaz Mithela 
Published: August 2024

Abstract

This study investigates the accessibility, gender disparities, and preferences related to extracurricular activities (ECAs) among students in Bangladesh. Utilizing a survey conducted with middle school, high school, and undergraduate students, we explore the challenges faced by urban versus rural students, the specific barriers encountered by female students, and the preferences for online versus offline ECAs. The results reveal significant disparities, with rural students and female participants facing considerable obstacles in accessing ECA opportunities. A strong preference for online ECAs is evident, although awareness of existing platforms facilitating ECA opportunities is limited. The findings highlight the need for targeted interventions to improve ECA accessibility, promote gender equality, and enhance the visibility of ECA resources. This study provides actionable recommendations to foster a more inclusive environment for ECA participation, ultimately contributing to the holistic development of students across Bangladesh.
Study on influencing factors of medication adherence to inhalers in patients with COPD and asthma based on Anderson Model
Authors: Yin Chengchen , Luo Yongyong , Shen Kaili , Mao Guangxin , Tang Fushan 
Published: August 2024

Abstract

Purpose
To investigate the influencing factors of adherence to inhalers in patients with COPD and asthma, providing reference for improving the adherence to inhalers of patients with asthma and COPD.
Methods
A questionnaire was designed based on Anderson's model, and used to investigate the influencing factors of adherence to inhalers in COPD or asthma patients who were discharged inpatients or outpatients and had previously been prescribed inhalers in the respiratory departments of three tertiary hospitals in Zunyi City.
Results
Only 25.90% of patients demonstrate good adherence to inhalers. When combined with the Anderson model, the influencing factors of inhaler adherence in COPD and asthma patients can be summarized as follows: contextual characteristics (residential status, management of chronic illness cards), individual characteristics (disease condition, family history, medication beliefs) and outcomes (health status).
Conclusion
The medication adherence to inhalers in patients with COPD and asthma falls far short of being satisfactory. Pharmacists should collaborate with physician, nurses, communities, and patients' families, to proactively implement interventions targeting influencing factors of medication adherence to inhalers in patients with COPD and asthma. This will help improve patients' adherence to inhalers and, subsequently, enhance their quality of life.
Denosumab combined with en bloc resection and arthrodesis for recurrent grade 3 giant cell tumor of bone in distal radius
Authors: Li Zhuoyu 
Published: August 2024

Abstract

Purpose
This study aimed to analyse the clinical outcomes of preoperative adjuvant denosumab therapy (PADT) combined with resection and arthrodesis for recurrent grade 3 giant cell tumor of bone (GCTB) in the distal radius.
Methods
A retrospective study was conducted on twenty-three patients (8 males, 15 females) who were treated with the adjuvant denosumab combined with en bloc resection (EBR) and arthrodesis for biopsy confirmed recurrent Campanacci III giant cell tumor of bone in the distal radius between January 2015 and December 2022. All 23 patients were treated with wrist arthrodesis reconstruction using autogenous free iliac crest bone graft (ICBG), bridging plate and screws. The local control, metastasis and overall survival were evaluated during the follow-up period. Functional outcomes were evaluated using the Disabilities of the Arm, Shoulder and Hand (DASH) score, Musculoskeletal Tumor Society Score (MSTS-87 and MSTS-93), and grip strength in the follow-up period. Additionally, all surgical or denosumab-related complications that occurred were recorded in this study.
Results
Twenty-three patients were included in this retrospective study and no patients were lost in the follow-up period. The average patient age was 32.5 ± 10.2 years (range, 19–53 years) and the mean follow-up time was 35.5 ± 18.4 months (range, 13–72 months). The average tumor length was 71.7 ± 8.7 mm (range, 50 to 85 mm) and bone reconstruction length was 78.5 ± 8.5 mm (range, 60 to 90 mm). Four patients (17.4%) had secondary local recurrence after reoperation and two patients had (8.7%) multiple recurrences. One patient (4.3%) was deceased in the last follow-up due to multiple metastases. The estimated 5-year recurrence-free survival rate was 81.3% and 5-year metastasis-free survival rate was 95.7%. The mean union time was 8.5 ± 1.9 (6–12) months and the overall survivorship of the allograft was 82.7% (21/23) at an average 35 month follow-up. The average MSTS-87 and MSTS-93 scores were 27.8 ± 1.6 (range, from 23 to 30) and 91.5 ± 5.0 (range, from 76 to 100), and the average DASH score was 8.9 ± 3.2 (range, from 3 to 15), respectively. The average grip strength was 64.6 ± 15.7% (range, from 30–95%) of the uninvolved side. Eight patients (34.7%) had at least one complication in the follow-up time. Two autografts (8.7%) were removed due to local recurrence and bone nonunion, and the average autograft survival time was 32.8 ± 18.5 months (range, 12 to 72 months).
Conclusions
Preoperative adjuvant denosumab therapy (PADT) combined with en bloc resection and arthrodesis is a promising method for the treatment of recurrent Campanacci III GCTB in distal radius with acceptable short-term local control and functional satisfaction.
Level of evidence
level IV Therapeutic.
The future bioenergy plant portfolio in the German power sector: scenarios for different framework conditions
Authors: Dotzauer Martin , Schindler Harry , Daniel-Gromke Jaqueline , Thraen Daniela 
Published: August 2024

Abstract

Bioenergy plays a crucial role in Germany’s renewable energy supply of 513 TWh in 2023. It contributes 254 TWh for heating, fuels, and electricity. The German Renewable Energy Sources Act (EEG) guarantees feed-in tariffs for electricity for 20 years. For many bioenergy plants, the feed-in tariff will expire in the coming years. The latest EEG of 2023 offers tenders, which are also open to existing plants to extend their operation for 10 years, but with lower subsidies. The fuel and heating markets also offer business opportunities that influence the portfolio dynamics of the German power sector.
We use an agent-based modelling approach to investigate the future development of the bioenergy plant portfolio under scenarios for different framework conditions. These include a number of business‑as‑usual cases, as well as 5 additional scenarios for the assessment of different policy measures.
We find that the current bioenergy portfolio in the German power sector cannot be maintained under the current framework conditions. Depending on the scenario, installed capacity will decline by between one third and one eight. Without appropriate adjustments, e.g. higher tender volumes, and 20 instead of 10 extended years of operation, Germany cannot reach its bioenergy target of 8.4 GW in 2030.
Nutrition Security Status of Women in Northwest Ethiopia
Authors: Teshome Fentaw , Andaregie Adino , Astatkie Tessema 
Published: August 2024

Abstract

Background: Nutrition security of women is one of the most vital foundations for the overall
development and wellbeing of society. Since understanding the factors that influence the nutrition
security of women is very important for implementing appropriate interventions, this study was
conducted to determine the nutrition security status of women in Northwest Ethiopia.
Methods: Survey data were collected from 197 rural women selected using a two- stage sampling
method (Cluster sampling in the first stage and Stratified random sampling in the second stage).
Data in the two outcomes (nutritionally secured whose BMI ≥ 18.5, and nutritionally insecure
whose BMI < 18.5 kg/m2) were analyzed using a Logit regression model to determine the
significance of the determinants on women’s nutrition security status.
Results: The study showed that 72.6% of the women in the study area were nutritionally insecure.
Results of the statistical analysis revealed that family size (negative effect); and the consumption
of milk, women’s daily feed frequency, fruits and vegetables, women empowerment, and animal
products have positive effect. The height, weight, and BMI of nutritionally secure women (50.8
kg, 1.55 m, and 21.1 kg/m2, respectively) were significantly higher than those of nutritionally
insecure women (44 kg, 1.45 m, and 15.8 kg/m2, respectively).
Conclusions: This is the first study on the determinants of rural women’s nutrition security status
using social and demographic data collected at the grassroots level and analyzed using an advanced
econometric model. The findings of the study show the need for government and other
stakeholders’ interventions to increase access to nutritious food products and to provide trainings
on feeding culture and dietary diversity to women. The findings of this study can help the
Government of Ethiopia to achieve its National Development Priorities.