bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the success rate of Microwave Ablation under the ultrasound guide in the treatment of Benign Thyroid Nodule
Authors: Babaei Mohammad Reza , Naserifard Akram , Heydari Iraj , Shafiee Nadia , Golmohammadi Sara , Shafiee Atefe , Taghizadeh Nima , Mohammadi Soha 
Published: June 2024

Abstract

Background
One of the common reasons for patients to visit the clinic for endocrine diseases is thyroid disorders, especially benign thyroid nodules. Different pharmacological and nonpharmacological strategies are used to treat benign thyroid nodules. This study was conducted to determine the success rate of ultrasound-guided microwave ablation (MWA) in the treatment of benign thyroid nodules.
Methods
In this descriptive/cross-sectional study, the number of 22 patients with benign thyroid nodules who were treated with MWA from 2017 to 2020 was examined 6 and 12 months after treatment in terms of nodule volume changes and side effects. The data were analyzed with SPSS.
Results
The average age of patients is 35.45 years. 90.9% were female and 9.1% were male. The average volume of benign thyroid nodules in the patients under study was 15.37 ± 8.67 before treatment, 8.25 ± 6.52 in the sixth month of treatment, and 5.12 ± 6.65 in the twelfth month of treatment (P < 0.001). Among the side effects of MWA, pain was reported by 1 person, pressure symptoms were reported by 1 person, and scarring occurred by 2 people.
Conclusions
MWA is an effective, successful, and relatively safe procedure for the treatment of benign thyroid nodules.
Testosterone and Estradiol's Protective Influence on Primary Liver Cancer
Authors: Hu Yuhai , ou wenshi , Yang Mingzhi , Chen Tianbin 
Published: June 2024

Abstract

Background:
Primary liver cancer (PLC) poses a significant global health threat, with sex hormones implicated in its pathogenesis. Testosterone and estradiol's influence on PLC risk remains debated. Mendelian randomization (MR) offers a robust method to assess causal relationships.
Methods:
We conducted MR analyses using genome-wide association study (GWAS) data to investigate the causal impact of testosterone and estradiol on PLC risk. Tumor necrosis factor receptor superfamily member 9(TNFRS9) and urokinase-type plasminogen activator(uPA) were explored as potential mediators.
Results:
Our MR analysis revealed a protective effect of testosterone and estradiol against PLC. Testosterone was negatively associated with PLC risk (OR = 0.9987, P = 0.0316), with TNFRS9 mediating 9.1% of this effect. Estradiol exhibited a negative correlation with PLC risk (OR = 0.9985, P = 0.0125), with uPA mediating 8% of this effect.
Conclusions:
Contrary to traditional beliefs, both testosterone and estradiol demonstrate protective effects against PLC. TNFRS9 and uPA pathways mediate a significant portion of these effects, providing novel insights into the sex hormone-PLC relationship and potential therapeutic targets. Further research should validate these findings across diverse populations.
Overexpression and infiltration of ER stress proteins in the sublining layer of human inflamed synovial membranes
Authors: Gendebien Zoe , Deroyer Celine , Poulet Christophe , Paulissen Genevieve , Cobraiville Gael , Daniel Christophe , Thirion Thierry , Bianchi Elettra , Delvenne Philippe , Ribbens Clio , de Seny Dominique 
Published: June 2024

Abstract

A strong crosstalk exists between endoplasmic reticulum (ER) stress and synovitis. Several ER chaperone proteins, besides their function in protein folding, can enhance inflammation and immunogenicity when secreted. This research aims at localizing and quantifying by immunohistochemistry (IHC) ER stress proteins (BiP, HYOU1, MANF, PDIA4, GANAB, HSP90B1, TXNDC5, DNAJB11, LMAN1, ERP29 and CALR) in synovial membranes provided from patients with osteoarthritis (OA; n = 9), chronic pyrophosphate arthropathy (CPPA; n = 7) and rheumatoid arthritis (RA; n = 8) and exhibiting continuous degree of inflammation. It also investigates ER protein expressions in fibroblast-like synoviocytes (FLS) under ER stress, pro-inflammatory and pro-fibrotic conditions. This study demonstrates the restricted localization of these proteins to the lining layer when inflammation was mild and in the whole synovium when the inflammation was severe. Differential expression of some ER stress proteins in synovitis was confirmed by using the CIOA mouse model. Tunicamycin-induced ER stress enhanced the intracellular protein expression of BiP, HYOU1, MANF, PDIA4, HSP90B1, LMAN1 and CALR in FLS and their extracellular secretion (except for HYOU1, MANF and LMAN1). Induction of inflammation with TNF-a upregulated BiP, HYOU1, MANF and PDIA4 expression, whereas exposure to the pro-fibrotic mediator TGF-b elevated expression of BiP, HYOU1, MANF and DNAJB11 in FLS.
Body Mass Index and the Prevalence of High-Risk Colorectal Adenomas in a Population Undergoing Screening Colonoscopy in Alberta, Canada
Authors: Hutchinson John M. , Chow Joshua , Farah Eliya , Warkentin Matthew T. , Ruan Yibing , Hilsden Robert J. , Brenner Darren R. 
Published: June 2024

Abstract

Purpose
There is limited evidence regarding body mass index (BMI) as an early marker of high-risk adenoma (HRA) at the time of screening colonoscopy. Because high-risk adenomas (HRA) can develop into colorectal cancer (CRC), BMI could serve as an important clinical predictor of future risk of CRC.
Methods
We examined data from 1,831 adults undergoing screening colonoscopy at the Forzani & MacPhail Colon Cancer Screening Centre in Alberta, Canada. We fit multivariable logistic regression models to examine the association between BMI and HRA. Non-linear relationships for BMI on HRA were also evaluated using restricted cubic splines.
Results
The mean BMI in patients with HRA was 28.2 kg/m2 compared to 27.4 kg/m2 in patients without adenomas (t-test: p = 0.003). In the adjusted models, those with a BMI over 30 kg/m2 had 1.44 (95% CI: 1.05–1.99) times the odds of HRA detected during colonoscopy compared to those with a BMI below 25 kg/m2. Examining BMI as continuous, the odds of HRA were 1.20 (95% CI: 1.05–1.37) times higher for every 5 kg/m2 increase in BMI.
Conclusion
The findings of this study suggest that excess body mass is associated with higher risk of HRA among a screening population and may be useful an early marker of future disease.
Hyperferritinemia screening to aid identification and differentiation of patients with hyperinflammatory disorders
Authors: Carol Hallie A. , Mayer Adam S. , Zhang Michael S. , Dang Vinh , Varghese Jemy , Martinez Zachary , Schneider Corinne , Baker Joy (Elizabeth) , Tsoukas Paul , Behrens Edward M. , Cron Randy Q. , Diorio Caroline , Henderson Lauren A. , Schulert Grant , Lee Pui , Kernan Kate F. , Canna Scott W. 
Published: June 2024

Abstract

High ferritin is an important and sensitive biomarker for hemophagocytic lymphohistiocytosis (HLH), a diverse and deadly group of cytokine storm syndromes. Early action to prevent immunopathology in HLH often includes empiric immunomodulation, which can complicate etiologic work-up and prevent collection of early/pre-treatment research samples. To address this, we instituted an alert system where serum ferritin > 1000ng/mL triggered real-time chart review, assessment of whether the value reflected “inflammatory hyperferritnemia (IHF)”, and biobanking of remnant samples from consenting IHF patients. We extracted relevant clinical data; periodically measured serum total IL-18, IL-18 binding protein (IL-18BP), and CXCL9; retrospectively classified patients by etiology into infectious, rheumatic, or immune dysregulation; and subjected a subgroup of samples to a 96-analyte biomarker screen. 180 patients were identified, 30.5% of which had IHF. Maximum ferritin levels were significantly higher in patients with IHF than with either hemoglobinopathy or transplant, and highly elevated total IL-18 levels were distinctive to patients with Stills Disease and/or Macrophage Activation Syndrome (MAS). Multi-analyte analysis showed elevation in proteins associated with cytotoxic lymphocytes in all IHF samples when compared to healthy controls and depression of proteins such as ANGPT1 and VEGFR2 in samples from hyperferritinemic sepsis patients relative to non-sepsis controls. This single-center, real-time IFH screen proved feasible and efficient, validated prior observations about the specificity of IL-18, enabled early sample collection from a complex population, suggested a unique vascular biomarker signature in hyperferritinemic sepsis, and expanded our understanding of IHF heterogeneity.
Patient Flow Management in Biological Events: A Scoping Review.
Authors: Hadinejad Zoya , Farrokhi Mehrdad , Saatchi Mohammad , Ahmadi Shokoufeh , Khankeh Hamidreza 
Published: June 2024

Abstract

Introduction: Biological Events affect large populations depending on transmission potential and propagation. A recent example of a biological event spreading globally is the COVID-19 pandemic, which has had severe effects on the economy, society, and even politics ,in addition to its broad occurrence and fatalities. The aim of this systematic scoping review was to look into patient flow management techniques and approaches used globally in biological incidents.Methods: The current investigation was conducted based on the guidelines of PRISMA for Scoping Review ,which is also used for systematic review and meta-analysis studies. All articles released until March 31, 2023, were examined for this study, regardless of the year of publication. The authores were searched in databases including Scopus, Web of Science, PubMed and Google scholar search engine. Papers with subjects and keywords about patient flow management and patient management policies during epidemics were included in the inclusion criteria, while all non-English language publications including those with only English abstracts were excluded.Results: A total of 19231 articles were included in this study and after screening, 36 articles were eventually entered into the final analysis. 84 primary categories and subcategories were identified from the review of pertinent studies. To facilitate more precise analysis and understanding, factors were categorised into seven categories: patient flow simulation models, risk communication management, integrated ICT system establishment, collaborative interdisciplinary and intersectoral approach, systematic patient management, promotion of health information technology models, modification of triage strategies, and optimal resource and capacity management.Conclusion:Patient flow management during biological Events plays a crucial role in maintaining the performance of the healthcare system. When public health-threatening biological incidents occur, due to the high number of patients, it is essential to implement a holistic ,and integrated approach from rapid identification to treatment and discharge of patients.
Is that mine? Ownership in autism spectre disorder
Authors: Yuan Yue , Tao Mingda 
Published: June 2024

Abstract

This study investigated the self-reference effect (SRE) in individuals with autism spectrum disorder (ASD) through the ownership paradigm across different task difficulties. Behavioral experiment results indicate that individuals with ASD retain the ability to process their sense of ownership in a simple item categorization task. However, deficits in the sense of ownership were observed when individuals with ASD engaged in a complex item categorization task. Eye-tracking experiments revealed no differences in eye-tracking outcomes related to the SRE in individuals with ASD during the first fixation duration. However, there were significant differences in eye-tracking outcomes related to the SRE for both simple and complex tasks in terms of ASD first-run dwell time and overall dwell time.
TRIM11 modulates sepsis progression by promoting HOXB9 ubiquitination and inducing the NF-κB signaling pathway
Authors: Gan Jiaqi , Zhang Wei , Pan Fei , QIU Zhiyun , Chen Xiaobing 
Published: June 2024

Abstract

Aim
The purpose of this investigation was to elucidate the functions of TRIM11 and HOXB9 in the pathogenesis of sepsis, focusing on their influence on inflammation, apoptosis, and the NF-κB signaling pathway.
Methods
Through public databases, TRIM family genes related to sepsis were screened, and TRIM11 was evaluated as a sepsis biomarker through ROC analysis. The UbiBrowser database screened TRIM11 downstream genes and identified HOXB9 as an important target. THP-1 cells were stimulated by Lipopolysaccharide (LPS) to induce inflammation and simulate sepsis. Flow cytometry, Enzyme-linked immunosorbent assay (ELISA), and Western blot (WB) experiments were used to detect changes in cell apoptosis rate, apoptosis-related proteins, and inflammatory cytokines after TRIM11 and HOXB9 were silenced. Additionally, we investigated the ubiquitination interaction between TRIM11 and HOXB9 and their effects on the NF-κB signaling pathway.
Results
Our findings demonstrated that sepsis patient samples had elevated levels of TRIM11 expression and had high clinical diagnostic value. Functional experiments showed that the knockdown of TRIM11 significantly alleviated LPS-induced THP-1 cell apoptosis and inflammation, while knockdown of HOXB9 did the opposite. Notably, the simultaneous downregulation of TRIM11 and HOXB9 balanced these responses, suggesting that they play a key role in regulating sepsis associated inflammation as well as apoptosis. In addition, TRIM11 regulated the NF-κB signaling pathway by reversing HOXB9-induced activation through ubiquitination, suggesting a novel regulatory mechanism in the pathogenesis of sepsis.
Conclusion
Our findings highlight the interaction between TRIM11 and HOXB9 in regulating inflammation and apoptosis pathways, providing new insights into sepsis treatment.
Association of Apolipoprotein E Gene Polymorphisms with Risk of Coronary Artery Disease in a Han Chinese Population at Middle and High Altitude in China
Authors: Zeng Fanrong , Zhang Xinyi , Zhang Meng , Liu Hongli , Li Yuan , Chen Xuejiao , Ye Fan , Zhou Lanting , Zhai Lihong , Zhu Fangyi 
Published: June 2024

Abstract

Background
This study aimed to assess how APOE gene polymorphisms affect the development of coronary artery disease (CAD) in the Han Chinese population at middle and high altitudes by regulating the body's lipid levels, which stimulates atherosclerosis.
Methods
A total of 628 CAD and non-CAD cases were enrolled in this study, respectively. APOE gene polymorphisms were determined by PCR and fluorescence hybridization techniques. Data analysis was performed using the software SPSS 26.0.
Results
This study revealed that the statistical differences in APOE gene ε3ε4 and ε4ε4 genotypes, as well as ε4 allele frequencies between the CAD and control groups, were significant (p < 0.05). Furthermore, Apo-B/Apo-A1, Apo-B, and LDL-C levels were statistically higher in CAD patients carrying the ε4 allele than in those carrying the ε2 and ε3 alleles. Moreover, multifactorial logistic regression analysis revealed that the APOE gene's ε3ε4 genotype (OR = 1.514, 95% CI = 1.087–2.109, p = 0.014) was the independent risk factor (IRF) for CAD in Han population at intermediate and high altitudes.
Conclusion
This study revealed that the APOE gene’s ε3ε4 genotype is a potential risk factor for the onset of CAD in the Han Chinese population at middle and high altitudes.
Rapid Diagnosis of Adult-type Diffuse Glioma using a Layered Scheme
Authors: Wu Jinsong , Wu Shuai , Cao Dandan , Xiong Zhang , Zhang Jianhua , Zou Yourui , Wu Zanyi , Nie Yanli , Yao Ye , Song Yanyan , Jiao Yuchen , Chen Hong , Ma Hui , Kang Dezhi , Mao Ying , Yan Hai 
Published: June 2024

Abstract

Background
Molecular biomarkers have become an essential part of the diagnosis of adult-type diffuse glioma. Still, complex detection methods and long-term turnaround for these biomarkers hinder integrated diagnosis in clinical practice. We hypothesized that IDH and TERTp mutations play similar roles in accurately classifying adult-type diffuse glioma compared to the complicated WHO CNS5-recommended biomarkers, and the detection of IDH and TERTp mutations should be the first layer in clinical practice.
Methods
We define a novel layered diagnostic scheme for adult-type diffuse gliomas with the detection of IDH and TERT promoter (TERTp) mutations as the first layer. Moreover, we develop a technology for rapid intraoperative testing of TERTp and IDH mutations within 35 mins. A retrospective cohort and a prospective multicenter diagnostic test were involved in. The diagnostic accuracy of the layered diagnostic scheme was measured via sensitivity, specificity, and the area under the receiver operating characteristic curve (AUC) with a 95% confidence interval.
Results
In retrospective cohort, the TERTp mutation displayed a similar statistical power as 1p/19q in distinguishing oligodendrogliomas from astrocytomas (kappa = 0.96, P < 0.001). In addition, 91.8% of all the glioblastomas with either EGFRamp or + 7/-10 had TERTp mutations. Moreover, prospective application of the layered diagnostic scheme and the rapid testing, 223 gliomas and 2 non-gliomas (76.5%) were classified accurately intraoperatively. With the assistance of the postoperative permanent section, 249 gliomas and 24 non-gliomas (92.9%) were accurately classified after detecting the first layer biomarkers.
Conclusions
The layered diagnostic scheme provides rapid and accurate integrated diagnoses of adult-type diffuse gliomas, facilitates the broader use of molecular classification, and expands its application from postoperatively to intraoperatively for most patients.
Molecular xenomonitoring as an indicator of microfilaraemia prevalence for lymphatic filariasis in Samoa in 2019
Authors: Howlett Maddison , Mayfield Helen , McPherson Brady , Rigby Lisa , Thomsen Robert , Williams Steven A , Pilotte Nils , Hedtke Shannon , Graves Patricia M. , Kearns Therese , Naseri Take , Sheridan Sarah , McLure Angus , Lau Colleen L 
Published: June 2024

Abstract

Background: Lymphatic filariasis (LF) is a globally significant, vector-borne, neglected tropical disease that can result in severe morbidity and disability. As the World Health Organization (WHO) Global Programme to Eliminate Lymphatic Filariasis makes progress towards LF elimination, there is greater need to develop sensitive, post-intervention surveillance. Molecular xenomonitoring (MX), the detection of pathogen DNA in vectors and other biting insects, may provide a sensitive complement to traditional human-based surveillance techniques, including detection of circulating filarial antigen and microfilaraemia (Mf). This study aims to explore the relationship between human Mf prevalence and the prevalence of PCR-positive mosquitoes using MX.
Methodology: We compared Mf and MX results from a 2019 community-based survey conducted in 35 primary sampling units (PSUs) in Samoa. We investigated concordance between presence and absence of PCR-positive mosquitoes and Mf-positive participants at the PSU level, and calculated sensitivity and negative predictive values for each indicator using presence of any Mf-indicated infection in humans or PCR-positive mosquitoes as a reference. Correlation between prevalence of filarial DNA in mosquitoes and Mf in humans was estimated at the PSU and household/trap level using mixed-effect Bayesian multilevel regression analysis.
Results: Mf-positive individuals were identified in less than half of PSUs in which PCR-positive mosquito pools were present (13 and 28, respectively). Prevalence of filarial DNA in mosquitoes (each species separately) was positively correlated with Mf prevalence in humans at the PSU level. Analysed at the species level, only Aedes polynesiensis demonstrated strong evidence of positive correlation (r) with human Mf prevalence at both PSU (r: 0.46, 95% CrI: 0.05–0.81) and trap/household levels (r: 0.57, 95% CrI: 0.16–0.87).
Conclusions: Our findings demonstrate that MX can be a sensitive surveillance method for identifying residual infection in low Mf prevalence settings. MX identified more locations with pathogen signals than Mf-testing. Strong correlation between estimated filarial DNA prevalence in the primary vector species and Mf in humans at small spatial scales demonstrates the utility of MX as an indicator for LF prevalence in Samoa and similar settings. Further investigation is needed to develop MX-based guidelines to strengthen the ability of MX to inform operational decisions.
Assessing cardiac function post chemotherapy combined with immune checkpoint inhibitors via automated two-dimensional speckle tracking
Authors: Wang Yiru , Sun Qiong , Gao Hanjing , Li Xiaoyan , Yang Weiwei , Shi Weiwei , Luo Yukun 
Published: June 2024

Abstract

Background
Although left ventricular (LV) dysfunction has been studied in patients with solid tumors and abnormal myocardial enzymes receiving immune checkpoint inhibitors, little is known about the early changes in LV strains and function in patients without significant markers of myocardial injury.
Methods
A total of 57 patients with solid tumors who received chemotherapy and immune checkpoint inhibitors were enrolled from December 2019 to June 2022. Echocardiography was performed at baseline and after 3 and 6 months. LVD, LVS, Volume D, Volume S, LVEF, Em, Am, E/A, E/e’, heart rate, and GLS were collected by 2 DE. Serum biomarkers of myocardial injury were tested at baseline and after 3 and 6 months. The discrimination of echocardiographic parameters and biomarkers between groups was statistically analyzed using SPSS version 19.0 software.
Results
A total of 49 patients (35 males; mean age ± SD, 57.5 ± 9.89 years) were included in this study. No significant differences were found in conventional ultrasound parameters, or HR throughout the follow-up period. The LA area decreased from 16.44 ± 4.17 at baseline to 15.13 ± 4.01 at 6-month follow-up (p < 0.001). Compared to baseline, the GLSs at the 3-month follow-up and 6-month follow-up were significantly reduced (p < 0.05). The creatine kinase and CK-MB levels showed an upward trend (p < 0.05). The levels of lactate dehydrogenase isoenzyme (p < 0.05) decreased at 3 months and increased at 6 months. E/e’ was negatively correlated with troponin T, creatine kinase, and blood glucose levels (r=-0.477, -0.258, -0.426, respectively; p < 0.05). GLS (3P) levels were positively correlated with creatine kinase (r = 0.017, p < 0.05). Patients were divided into two groups based on the presence (G1,15 cases) of other system immune responses or absence (G0, 34 cases) of other system immune responses. There were statistical differences in EF values among different time points (p < 0.05). There was no statistically significant difference in left ventricular strain parameters between the G0 and G1 groups (p > 0.05), but significant differences were observed at different time points (p < 0.05).
Conclusions
The evaluation of left ventricular strain parameters by echocardiography is of great significance for early detection of myocardial toxicity caused by immunotherapy without evidence of elevated myocardial enzymes.
Trial registration
: retrospectively registered