bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Dynamics of TAT Process
Authors: Verdon Benoît , Chabert Catherine , Azoulay Catherine , Emmanuelli Michèle , Neau Françoise , Vibert Sarah , Louët Estelle 
Published: December 2014

After many years of clinical practice, research and the teaching of projective tests, Shentoub and her colleagues (Debray, Brelet, Chabert & al.) put forward an original and rigorous method of analysis and interpretation of the TAT protocols in terms of psychoanalysis and clinical psychopathology. They developed the TAT process theory in order to understand how the subject builds a narrative.Our article will emphasize the source of the analytical approach developed by V. Shentoub in the 1950s to current research; the necessity of marking the boundary between the manifest and latent content in the cards; the procedure for analyzing the narrative, supported by an analysis sheet for understanding the stories' structure and identifying the defense mechanisms; and how developing hypotheses about how the mental functions are organized, as well as their potential psychopathological characteristics; and the formulation of a diagnosis in psychodynamic terms.In conjunction with the analysis and interpretation of the Rorschach test, this approach allows us to develop an overview of the subject's mental functioning, taking into account both the psychopathological elements that may threaten the subject and the potential for a therapeutic process. We will illustrate this by comparing neurotic, borderline, and psychotic personalities.
Quantum delocalization of protons in the hydrogen-bond network of an enzyme active site
Authors: Wang Lu , Fried Stephen D. , Boxer Steven G. , Markland Thomas E. 
Published: December 2014

Significance
Because of the low mass of the proton, nuclear quantum effects can dramatically alter the properties of hydrogen-bond networks, especially when short and strong hydrogen bonds occur. Here, we combine experiments and state-of-the-art simulations that include the quantum nature of both the electrons and nuclei to show that the enzyme ketosteroid isomerase contains a hydrogen-bond network in its active site that facilitates extensive quantum proton delocalization. This leads to a 10,000-fold increase in the acidity of an active-site residue compared with the limit where the nuclei are classical particles. This work opens up new avenues for understanding the interplay between quantum effects and hydrogen bonding in biological systems containing strong hydrogen bonds.
Lack of Protection following Re-Exposure of Chinook Salmon to Ceratonova shasta (Myxozoa)
Authors: Hurst C. N. , Bartholomew J. L. 
Published: December 2014

Abstract
The recent identification of multiple genotypes of the salmonid parasite Ceratonova shasta with different virulence levels in Chinook Salmon Oncorhynchus tshawytscha suggests that it is possible to immunize fish against subsequent infection and disease. We hypothesized that exposure of Chinook Salmon to the less-virulent parasite genotype (II) prior to the more virulent parasite genotype (I) would decrease disease and/or result in fewer mature parasites compared with fish only infected with the more virulent genotype. To test this hypothesis, fish were challenged in a combination of field and laboratory exposures, and we measured infection prevalence, percent morbidity, and mature parasite production. Neither mortality nor mature parasite production were reduced when fish were exposed to genotype II prior to genotype I compared with fish exposed only to genotype I, suggesting that protection against C. shasta using a less-virulent genotype of the parasite does not occur.
Received June 9, 2014; accepted September 19, 2014
Plasma Renin Activity and Resting Heart Rate in a Population of Community-Dwelling Japanese: The Tanushimaru Study
Authors: Adachi Hisashi , Enomoto Mika , Fukami Ako , Kumagai Eita , Nakamura Sachiko , Yoshimura Ayako , Obuchi Aya , Hori Kensuke , Nohara Yume , Nakao Erika , Fukumoto Yoshihiro 
Published: December 2014

BACKGROUND
Heart rate is a strong predictor of mortality and development of obesity and diabetes. The renin–angiotensin–aldosterone system plays an important role in blood pressure control and volume homeostasis. Although many studies have indicated the association between aldosterone and hypertension or insulin resistance, epidemiological evidence of the association of heart rate with plasma renin activity (PRA) remains scant. Therefore, we investigated whether heart rate is associated with PRA.

METHODS
A total of 1,943 subjects were enrolled, who underwent a health examination in Tanushimaru in 2009. Plasma renin and aldosterone concentrations were measured by radioimmunoassay. PRA and the homeostasis model assessment (HOMA) were used by natural-log transformed. Resting heart rate was measured using electrocardiography.

RESULTS
We divided the subjects into four groups by heart rate (&lt;60/min, 60–69/min, 70–79/min, ≥80/ min), and analyzed an association between PRA and heart rate by analysis of covariance after adjustments for age and sex. The adjusted mean PRA and HOMA index showed a significant trend (P &lt; 0.01) as higher heart rate, although there was no significant trend between aldosterone and heart rate (P = 0.26). In multiple linear regression analysis adjusted for age, sex, systolic blood pressure, HOMA index, and hypertensive medication, PRA was positively and strongly associated with elevated heart rate (P &lt; 0.01).

CONCLUSIONS
This epidemiological study demonstrated that PRA, but not aldosterone, is significantly and positively associated with higher resting heart rate in a general population.
Heme Oxygenase Improves Renal Function by Potentiating Podocyte-Associated Proteins in Nω-Nitro-l-Arginine-Methyl Ester (l-NAME)-Induced Hypertension
Authors: Ndisang Joseph Fomusi , Chibbar Rajni 
Published: December 2014

BACKGROUND
Although heme-oxygenase (HO) is cytoprotective, its effects on podocyte regulators like podocalyxin, podocin, CD2-associated protein (CD2AP) in renal dysfunction in Nω-nitro-l-arginine-methyl ester (l-NAME) hypertension are largely unclear.

METHODS
Hypertension was induced in normotensive Sprague Dawley rats by administering l-NAME for 4 weeks. Enzyme immunoassay, enzyme-linked immunosorbent, histology/morphology, spectrophotometry, and western immunoblotting were used. HO was enhanced with heme-arginate (HA) or inhibited with chromium mesoporphyrin (CrMP).

RESULTS
Treatment with heme-arginate reduced several renal histo-pathological lesions including renal arteriolar thickening, glomerular abnormalities, tubular cast, tubular atrophy/fibrosis, and mononuclear cell infiltration in l-NAME-hypertensive rats. Similarly, HA abated the elevated levels of renal extracellular matrix/profibrotic proteins like collagen and fibronectin that deplete nephrin, a fundamental transmembrane protein that forms the scaffoldings of the podocyte slit diaphragm permitting small ions to filter, but not massive excretion of proteins, hence proteinuria. Correspondingly, HA enhanced the aberrant expression of nephrin alongside other important regulators of podocyte like podocalyxin, podocin, and CD2AP, and improved renal function by reducing albuminuria/proteinuria, while increasing creatinine clearance. The renoprotection by HA were accompanied by significant reduction of inflammatory/oxidative mediators including nuclear factor-kappaB, macrophage inflammatory protein-1-alpha, macrophage chemoattractant protein-1, tumor necrosis factor-alpha, interleukin (IL)-6, IL1β, 8-isoprostane, endothelin-1, and aldosterone. These were associated with increased levels of adiponectin, HO-1, HO activity, cyclic guanosine monophosphate, and atrial natriuretic peptide (ANP), whereas the HO inhibitor, CrMP annulled the renoprotection and exacerbated renal dysfunction.

CONCLUSIONS
HA improves renal function by attenuating histopathological lesions, suppressing inflammatory/oxidative mediators, abating profibrotic/extracellular matrix proteins, and reducing albuminuria/proteinuria, while concomitantly potentiating the HO-adiponectin-ANP axis, enhancing nephrin, podocin, podocalyxin, CD2AP and increasing creatinine clearance. Our study underscores the benefit of potentiating the HO-adiponectin-ANP against nephropathy.
Elevated Homocysteine Levels Are Associated With the Metabolic Syndrome and Cardiovascular Events in Hypertensive Patients
Authors: Catena Cristiana , Colussi Gianluca , Nait Francesca , Capobianco Frine , Sechi Leonardo A. 
Published: December 2014

BACKGROUND
Hyperhomocysteinemia and the metabolic syndrome are established cardiovascular risk factors and are frequently associated with hypertension. The relationship of plasma homocysteine (Hcy) with the metabolic syndrome and insulin resistance, however, is debated and studies in hypertensive patients are limited. In this study, we have investigated the association of Hcy with the metabolic syndrome and cerebro- cardiovascular events in hypertension.

METHODS
In 562 essential hypertensive patients who underwent accurate assessment of fasting and postload glucose metabolism, insulin sensitivity, and renal function, we measured plasma levels of Hcy, vitamin B12, folate, and fibrinogen and assessed the prevalence of the metabolic syndrome and of coronary heart and cerebrovascular disease (CVD).

RESULTS
Patients with the metabolic syndrome had significantly higher plasma Hcy levels. After correction for covariates, increasing Hcy levels were associated with an increasing prevalence of the metabolic syndrome, coronary heart disease, and CVD. Plasma Hcy was directly correlated with age, waist circumference, fasting glucose, triglyceride, uric acid, and fibrinogen levels, and homeostatic model assessment index and inversely with creatinine clearance and high-density lipoprotein cholesterol, vitamin B12, and folate levels. Logistic regression analysis showed an independent association of Hcy levels with age, male gender, vitamin B12 and folate levels, and the metabolic syndrome. Logistic regression indicated also an independent association of Hcy with cerebro-cardiovascular disease that was independent of the metabolic syndrome.

CONCLUSIONS
Elevated plasma Hcy is associated with the metabolic syndrome in hypertensive patients. Prevalence of events increases with increasing plasma Hcy levels suggesting a contribution of Hcy to cerebro-cardiovascular diseases in these patients.
Subtherapeutic concentrations of first-line anti-TB drugs in South African children treated according to current guidelines: the PHATISA study
Authors: Hiruy Hiwot , Rogers Zoe , Mbowane Chris , Adamson John , Ngotho Lihle , Karim Farina , Gumbo Tawanda , Bishai William , Jeena Prakash 
Published: December 2014

Abstract

Objectives
There is a paucity of evidence regarding the optimal dosing of anti-TB drugs in children. The aim of this study was to identify the pharmacokinetic parameters of first-line anti-TB drugs and the concentrations achieved after implementation of the 2010 WHO-recommended paediatric dosages.

Methods
We conducted a prospective, observational pharmacokinetic study in children 10 years old or younger who were on isoniazid, rifampicin, pyrazinamide and ethambutol therapy in Durban, KwaZulu-Natal, South Africa. Blood was collected at six timepoints over a 24 h period, chosen using optimal sampling theory. The drug concentrations were simultaneously modelled to identify the compartmental pharmacokinetics of each drug in each child, using the ADAPT program.

Results
The best six sampling timepoints in children were identified as 0 (pre-dose) and 0.42, 1.76, 3.37, 10.31 and 24 h post-dose. Thirty-one children were recruited and blood was drawn at these timepoints. Rifampicin, ethambutol and pyrazinamide were best described using a one-compartment model, while isoniazid was best described with a two-compartment model. Only 2/31 (6%), 20/31 (65%), 17/31 (55%) and 2/13 (15%) of children attained the WHO 2 h target therapeutic concentrations of rifampicin, isoniazid, pyrazinamide and ethambutol, respectively. Moreover, only 24/31 (77%), 6/31 (19%) and 8/31 (26%) achieved the AUCs associated with an optimal clinical response to rifampicin, pyrazinamide and isoniazid, respectively. No single risk factor was significantly associated with below-normal drug levels.

Conclusions
The drug concentrations of all first-line anti-TB drugs were markedly below the target therapeutic concentrations in most South African children who received the revised WHO-recommended paediatric weight-based dosages.
Multifaceted intervention to optimize antibiotic use for intra-abdominal infections
Authors: Popovski Zagorka , Mercuri Mathew , Main Cheryl , Sne Niv , Walsh Kate , Sung Melani , Rice Timothy , Mertz Dominik 
Published: December 2014

Abstract

Objectives
Implementing evidence-based practice guidelines is challenging. We used a multifaceted, continuous educational approach to disseminate an up-to-date internal guideline adapted from published guidelines for management of intra-abdominal infections (IAI).

Patients and methods
The intervention consisted of continuing educational sessions, internal guideline pocket cards and posters with collaboration among all key stakeholders starting in December 2010. We emphasized risk stratification and the use of ceftriaxone/metronidazole for treatment of low-risk IAI, and discouraged the use of fluoroquinolones due to the high local resistance rates. We then compared patients with IAI before the intervention (April–November 2010) to those after implementation of the guideline (April–November 2011) in a surgical unit at a tertiary care teaching hospital in Hamilton, Ontario, Canada. Antibiotic use was measured in in-hospital days of antibiotic therapy (DOT) per 1000 patient days (PD).

Results
152 and 145 patients with IAI were included in the pre- and post-intervention periods, respectively. There was a significant reduction in the proportion of patients who received ciprofloxacin therapy from 74% to 34% (OR 0.18, 95% CI 0.11–0.31) and in DOT/1000 PD from 221 to 74 (OR 0.3, 95% CI 0.2–0.3). Also, a reduction in the DOT/1000 PD for piperacillin/tazobactam was seen (from 116 to 67; OR 0.6, 95% CI 0.5–0.7). There was an increase in the use of ceftriaxone from 1.3% to 53% of patients (OR 85, 95% CI 20–515) and from 6 to 92 DOT/1000 PD (OR 17, 95% CI 10–25). This change in practice was sustained over &gt;2 years since the end of the active intervention, as shown in the unit-wide antimicrobial utilization data.

Conclusions
A multifaceted intervention aimed at all key stakeholders resulted in a high adherence to evidence-based treatment guidelines for IAI and has initiated a sustained culture change in prescribing of antibiotics.
Histoplasmosis in Patients With Cell-Mediated Immunodeficiency: Human Immunodeficiency Virus Infection, Organ Transplantation, and Tumor Necrosis Factor-α Inhibition
Authors: Luckett Keith , Dummer J. Stephen , Miller Geraldine , Hester Sydney , Thomas Lora 
Published: December 2014

Abstract
Background.  Histoplasmosis causes severe disease in patients with defects of cell-mediated immunity. It is not known whether outcomes vary related to the type of immunodeficiency or class of antifungal treatment.
Methods.  We reviewed cases of active histoplasmosis that occurred at Vanderbilt University Medical Center from July 1999 to June 2012 in patients with human immunodeficiency virus (HIV) infection, a history of transplantation, or tumor necrosis factor (TNF)-α inhibitor use. These groups were compared for differences in clinical presentation and outcomes. In addition, outcomes were related to the initial choice of treatment.
Results.  Ninety cases were identified (56 HIV, 23 transplant, 11 TNF-α inhibitor). Tumor necrosis factor-α patients had milder disease, shorter courses of therapy, and fewer relapses than HIV patients. Histoplasma antigenuria was highly prevalent in all groups (HIV 88%, transplant 95%, TNF-α 91%). Organ transplant recipients received amphotericin B formulation as initial therapy less often than other groups (22% vs 57% HIV vs 55% TNF-α; P = .006). Treatment failures only occurred in patients with severe disease. The failure rate was similar whether patients received initial amphotericin or triazole therapy. Ninety-day histoplasmosis-related mortality was 9% for all groups and did not vary significantly with choice of initial treatment.
Conclusions.  Histoplasmosis caused milder disease in patients receiving TNF-α inhibitors than patients with HIV or solid organ transplantation. Treatment failures and mortality only occurred in patients with severe disease and did not vary based on type of immunosuppression or choice of initial therapy.
Transmission of Respiratory Syncytial Virus Infection Within Families
Authors: Heikkinen Terho , Valkonen Heikki , Waris Matti , Ruuskanen Olli 
Published: December 2014

Abstract
Background.  Because the production of an effective respiratory syncytial virus (RSV) vaccine for infants is challenging, vaccination of other family members is one viable alternative to prevent severe RSV illnesses in infants.
Methods.  In a prospective study, we enrolled all family members of children who were hospitalized with RSV infection. Nasal swabs for RSV detection were obtained from all participating family members. Data on respiratory symptoms in the family members prior to and after the child's admission were collected using standardized questionnaires.
Results.  At the time of or within 1 week after the index child's hospitalization, RSV was detected in 40 (77%) of the 52 families and in 60 (47%) of 129 family members. Forty-nine (82%) of RSV detections in the family members were associated with respiratory symptoms. A sibling or a parent was the probable primary case of RSV in 30 (58%) families. Respiratory syncytial virus loads in the nasal swabs were significantly higher (107.7) in index children than in their parents (105.1, P &lt; .0001).
Conclusions.  In most cases, the likely source of an infant's RSV infection is an older sibling or a parent. These findings support the strategy of reducing the burden of RSV in infants by vaccination of their family members.
The Avian RNAseq Consortium: a community effort to annotate the chicken genome
Authors: Smith Jacqueline , Burt David W. ,   the Avian RNAseq Consortium
Published: December 2014

AbstractHere we describe how members of the chicken research community have come together as the “Avian RNAseq Consortium” to provide chicken RNAseq data with a view to improving the annotation of the chicken genome. The data was used by Ensembl in their release 71 gene_build to help provide the most up-to-date annotation of Galgal4, which is still in current use. The data has also been used to predict many ncRNAs, particularly lncRNAs, and it continues to be used as a resource for annotation of other avian genomes along with continued gene discovery in the chicken. This article is submitted as part of the Third Report on Chicken Genes and Chromosomes which will be published in Cytogenetic and Genome Research
Pre-synaptic inhibition of afferent feedback in the macaque spinal cord does not modulate with cycles of peripheral oscillations around 10 Hz
Authors: Galán Ferran , Baker Stuart N 
Published: December 2014

AbstractSpinal interneurons are partially phase-locked to physiological tremor around 10Hz. The phase of spinal interneuron activity is approximately opposite to descending drive to motoneurons, leading to partial phase cancellation and tremor reduction. Pre-synaptic inhibition of afferent feedback modulates during voluntary movements, but it is not known whether it tracks more rapid fluctuations in motor output such as during tremor.In this study, dorsal root potentials (DRPs) were recorded from the C8 and T1 roots in two macaque monkeys following intra-spinal micro-stimulation (random inter-stimulus interval 1.5-2.5 s, 30-100μA), whilst the animals performed an index finger flexion task which elicited peripheral oscillations around 10Hz. Forty one responses were identified with latency <5ms; these were narrow (mean width 0.59 ms), and likely resulted from antidromic activation of afferents following stimulation near terminals. Significant modulation during task performance occurred in 16/41 responses, reflecting terminal excitability changes generated by pre-synaptic inhibition (Wall’s excitability test). Stimuli falling during large-amplitude 8-12Hz oscillations in finger acceleration were extracted, and sub-averages of DRPs constructed for stimuli delivered at different oscillation phases. Although some apparent phase-dependent modulation was seen, this was not above the level expected by chance.We conclude that although terminal excitability reflecting pre-synaptic inhibition of afferents modulates over the timescale of a voluntary movement, it does not follow more rapid changes in motor output. This suggests that pre-synaptic inhibition is not part of the spinal systems for tremor reduction described previously, and that it plays a role in overall – but not moment-by-moment – regulation of feedback gain.
HISAT: Hierarchical Indexing for Spliced Alignment of Transcripts
Authors: Kim Daehwan , Langmead Ben , Salzberg Steven L. 
Published: December 2014

AbstractHISAT is a new, highly efficient system for alignment of sequences from RNA sequencing experiments that achieves dramatically faster performance than previous methods. HISAT uses a new indexing scheme, hierarchical indexing, which is based on the Burrows-Wheeler transform and the Ferragina-Manzini (FM) index. Hierarchical indexing employs two types of indexes for alignment: (1) a whole-genome FM index to anchor each alignment, and (2) numerous local FM indexes for very rapid extensions of these alignments. HISAT’s hierarchical index for the human genome contains 48,000 local FM indexes, each representing a genomic region of ~64,000 bp. The algorithm includes several customized alignment strategies specifically designed for mapping RNA-seq reads across multiple exons. In tests on a variety of real and simulated data sets, we show that HISAT is the fastest system currently available, approximately 50 times faster than TopHat2 and 12 times faster than GSNAP, with equal or better accuracy than any other method. Despite its very large number of indexes, HISAT requires only 4.3 Gigabytes of memory to align reads to the human genome. HISAT supports genomes of any size, including those larger than 4 billion bases. HISAT is available as free, open-source software fromhttp://www.ccb.jhu.edu/software/hisat.