bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cidadania Cultural LGBT
Authors: Colaço Rodrigues Rita de Cassia 
Published: July 2024

Este artigo discute a não efetividade da cidadania cultural no que respeita Ã população LGBT. Realiza breve historicidade acerca da memória, com a sua elevação, juntamente com a história, Ã patrimônio cultural, integrante dos direitos fundamentais, e aborda a sua tutela legislativa. Problematiza a invisibilidade de atores singulares do segmento nos espaços de memória da cidade. Denuncia a destruição sistemática de fontes históricas; a prevalência da noção exclusivamente privada nos acervos culturais; a ausência de canais institucionais de efetiva interlocução entre as instituições detentoras de arquivos e acervos e para a participação da população, falhando o estado brasileiro no seu dever constitucional. Discute a ainda parca preocupação do segmento em lutar pela sua preservação e acessibilidade. Inventaria iniciativas comunitárias em acervos, pesquisa e museologia. Conclui apontando algumas sugestões e destacando a função estratégica da história, memória e cultura como mecanismo de reparação e inclusão.
experiência de si
Authors: Souza Leme Erika , Holmes Brazil Christina , Andrade Lusimar , Souza de Cerqueira Paula Joana 
Published: July 2024

A pesquisa "Formação Cultural e Educação inclusiva: ampliando horizontes e diminuindo barreiras"[1], baseada na perspectiva da Teoria Crítica, criou o Portfólio “Escrita de Si” a partir de produções culturais, que provocam o pensamento crítico e a abordagem sensível da vida, com vistas à formação de si. Trata-se de um produto teórico-metodológico que engloba a formação de subjetividades inclusivas ancoradas nas dimensões de acessibilidade e sensibilidade. Assim, tem como objetivo apreender os sentidos e os significados experienciados por todas as vidas humanas, independentemente de suas especificidades, no contexto da pandemia da COVID-19. Operando com a contingência desta crise humanitária, o Portfólio se configura como um instrumento de resistência, por possibilitar experiências de sentir/pensar, que configuram um processo de entendimento, de conhecimento e de formação, possibilitando que as experiências sejam significativas e reverberem em ações, dando sentido ao que somos e ao que nos acontece.

[1] Pesquisa contemplada com bolsa de iniciação científica da Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro - FAPERJ.
construção social de tempo baseado em eventos nas culturas indígenas Awetý, Kamaiurá e Huni Kuĩ
Authors: Da Silva Sinha Vera , Kamaiura Sabino Wary , Kaxinawa Joaquim 
Published: July 2024

Nas culturas Huni Kuĩ, Awetý, Kamaiurá, o tempo é concebido em termos de eventos sociais e ambientais; linguisticamente, tal concepção se materializa em palavras e expressões usadas no cotidiano, em atividades e práticas culturais, quando os falantes se referem a fatos que necessitam de marcas temporais, como planejar festas, plantios, colheitas e até a organização de um dia típico qualquer. Nesse contexto, o presente estudo trata de como se dá a lexicalização e a indexação de intervalos de tempo nas línguas e culturas enfocadas, através de um inventário linguístico de marcas temporais relativas aos estágios da vida, divisões do dia e da noite e intervalos sazonais que norteiam a vida social. Para tanto, o texto discorre sobre como o sol, a lua e as estrelas estão inseridos no processo de indexicalização de tempo baseado em eventos; como números, mãos, dedos, nós em uma corda e marcas em madeira são utilizados como indexicalizadores temporais; e como calendários híbridos e outros aspectos culturais representam intervalos de tempo baseados em eventos nessas línguas e culturas.
KLASTERISASI MINAT GENERASI Z TERHADAP WAKAF HIJAU BERDASARKAN DEMOGRAFI
Authors: Notolegowo Hantoro Ksaid 
Published: July 2024

Potensi wakaf di Indonesia terbilang cukup besar. Namun potensi wakaf tersebut belum dapat dimanfaatkan secara optimal karena realisasinya belum mencapai target. Rendahnya minat masyarakat dalam berwakaf menjadi salah satu penyebab masih belum terpenuhinya realisasi wakaf. Saat ini tengah dikembangkan wakaf hijau, yang merupakan salah satu jenis wakaf yang diharapkan mampu meningkatkan minat masyarakat dalam berwakaf, karena pemanfaatannya yang mengakomodasi prinsip pelestarian lingkungan. Salah satu kelompok masyarakat yang berpotensi dalam pemanfaatan wakaf hijau adalah generasi z. Penelitian ini bertujuan untuk melakukan klasterisasi minat generasi z terhadap wakaf hijau berdasarkan profil demografi (gender, pendidikan, dan pendapatan). Dari hasil analisis, menunjukkan bahwa generasi z perempuan memiliki minat yang lebih tinggi terhadap wakaf hijau dibandingkan generasi z laki-laki. Generasi z dengan jenjang pendidikan dan tingkat pendapatan tertentu menunjukkan minat yang beragam terhadap wakaf hijau.
ANALISIS KEMAMPUAN PEMECAHAN MASALAH MATEMATIS MATERI POLA BILANGAN PADA SISWA KELAS VIII SMP KATOLIK AMBON
Authors: Tharob Max James , Palinussa Anderson Leonardo , Tamalene Hanisa 
Published: July 2024

Penelitian ini merupakan penelitian kualitatif yang bertujuan untuk mendeskripsikan tingkat kemampuan pemecahan masalah matematis siswa kelas VIII SMP Katolik Ambon pada materi pola bilangan. Subjek dalam penelitian ini sebanyak 3 siswa yang masing-masing mewakili kategori kemampuan pemecahan masalah tinggi, sedang dan rendah. Teknik pengumpulan data yang digunakan yaitu tes tertulis, wawancara dan dokumentasi. Hasil penelitian menunjukkan bahwa siswa dengan kemampuan pemecahan masalah tinggi dapat memenuhi setiap indikator kemampuan pemecahan masalah. Siswa berkemampuan sedang dapat memenuhi indikator kemampuan pemecahan masalah yang pertama, ketiga dan keempat. Sedangkan subjek penelitian dengan kemampuan rendah hanya dapat memenuhi indikator pertama dari empat indikator kemampuan pemecahan masalah.

Kata kunci: kemampuan pemecahan masalah, pola bilangan.
How is the Usage of the Swiss Variety of Italian Perceived in the Educational Context?
First Outcomes of the Project Repertorio Lessicale dei Regionalismi d’Uso Scolastico della Svizzera Italiana
Authors: Cignetti Luca , Baranzini Laura , Fornara Simone , Manetti Elisa Désirée 
Published: July 2024

RepSi (acronym standing for Repertorio lessicale dei regionalismi d’uso scolastico della Svizzera italiana) is a project that aims at collecting and analyzing up-to-date data about the perception and the usage of the Swiss variety of Italian (ISIT) in the educational context in the Italian-speaking Regions of Switzerland, namely Ticino and Graubünden. This contribution is divided in two main sections: the first one intends to explain how the project RepSi has been developed and which milestones have already been reached during the first year of work; the second one presents a selection of words (explained both in their meaning and in their use) and some further developments related to school teaching and perception of this regional variety of language.
HUBUNGAN PERAWATAN LUKA DAN KEPATUHAN MINUM OBAT UNTUK PENYEMBUHAN SIRKUMSISI DI KLINIK AINUN MAREZA PERCUT
Authors: Siringo-ringo Boy Rizky Anriano , Lubis Siska Anggreni , Hasibuan Hardy , Sitepu John Frans , Lubis Tifanni Tantina 
Published: July 2024

Sirkumsisi merupakan operasi pengangkatan preputium pada penis. Penyembuhan luka merupakan proses alami tubuh dalam memperbaiki atau mengembalikan integritas jaringan yang rusak atau terluka. Penyembuhan luka dapat dipengaruhi oleh obat dan kebersihan diri karena dapat meminimalkan risiko infeksi atau mengurangi peradangan sehingga penyembuhan lebih cepat terjadi. Tujuan pada penelitian ini untuk mengetahui hubungan antara perawatan luka dan kepatuhan meminum obat dengan lama penyembuhan luka post sirkumsisi metode konvensional di Klinik Ainun Mareza Penelitian ini bersifat analitik dengan desain cross sectional dengan pengambilan data menggunakan data primer berupa kuesioner untuk mengukur perawatan luka dan kepatuhan meminum obat, sedangkan lama penyembuhan luka sirkumsisi diukur dengan cara observasi. Teknik pengambilan sampel merupakan purposive sampling dan didapatkan sebanyak 38 responden di Klinik Ainun Mareza. Hasil uji gamma terhadap kepatuhan meminum obat-obatan dengan lama penyembuhan luka diperoleh nilai signifikansi p=0,00. yang artinya lebih kecil dari = 0,05. Hasil uji gamma terhadap perawatan luka dengan lama penyembuhan luka diperoleh nilai signifikansi (p) = 0,001 yang artinya lebih kecil dari = 0,05. Terdapat hubungan antara perawatan luka dan kepatuhan meminum obat dengan lama penyembuhan luka di Klinik Ainun Mareza.
Practical Aspects of Assessing Toxic Lesions of the Peripheral Nervous System in Preclinical Studies in Rodents: A Review
Authors: Ilinskii N. S. , Tyunin M. A. , Chepur S. V. , Pugach V. A. , Myasnikov V. A. 
Published: July 2024

INTRODUCTION. In the current practice of preclinical safety studies of pharmacologically active substances, standard neurotoxicity assessment procedures are mainly aimed at diagnosing higher nervous activity and behavioural disorders. However, it is the structures of the peripheral nervous system that are particularly susceptible to drug-induced neurotoxicity, which renders these structures an easy target and leads to a high incidence of neurotoxic side effects. These circumstances dictate the importance of refining methodological approaches to the assessment of toxic injury in the peripheral nervous system.AIM. The study aimed to analyse the current methodological level of clinical and functional tests for assessing the toxic effects of pharmacologically active substances on the structures of the peripheral nervous system, as well as to formulate practical recommendations for using these tests in preclinical studies in rodents.DISCUSSION. Rodents are considered the optimal test system for preclinical studies of pharmacologically active substances, but it is impossible to reproduce the entire neurological examination that is conducted to identify clinical equivalents of neurotoxicity in humans using these animals. This article presents a systematic approach to using available diagnostic tests to increase the translatability of data. The article briefly describes the neurological deficits due to adverse drug reactions in humans, as well as the main toxidromes that can also occur in animals. Based on a literature review and experience, the authors provide practical recommendations for performing basic tests to study the strength and tone of muscles, the state of physiological reflexes, the coordination of movements, and various types of sensitivities in rodents. The article provides a brief overview of the diagnostic utility of electrophysiological testing for identifying toxic damage to the peripheral nervous system. The following tests are recommended as a minimum list of primary screening techniques for detecting neurotoxic side effects in study animals: a resting posture assessment, the beam walking test, the horizontal bar test, the digit abduction score assay, the tail flick test, and the Preyer reflex test.CONCLUSIONS. The results of a comprehensive assessment of neurological deficits in rodent experiments should be analysed from a clinically relevant perspective— that is, with a focus on topical diagnosis and common pathological process components. It is advisable to verify the pathological process at the level of the peripheral nervous system using a set of electrophysiological techniques.
Complex Assessment of the Functional State of the Urinary System in Preclinical Studies. Part 1. Instrumental and Laboratory Assessment Methods (Review)
Authors: Miroshnikov M. V. , Sultanova K. T. , Makarova M. N. , Faustova N. M. , Khan S. O. , Loseva E. A. 
Published: July 2024

INTRODUCTION. Functional examination of the urinary system, and particularly the kidneys, is an important challenge in preclinical studies. Currently, there is no generally recognised and detailed approach to drug-induced nephrotoxicity detection in vivo, nor are there clear criteria for its assessment.AIM. This study aimed to analyse and systematise instrumental and laboratory methods for the assessment of urinary system function in laboratory animals and to identify the basic principles for studying drug-induced nephrotoxic effects.DISCUSSION. The study analysed the advantages and limitations of the methods used to study the nephrotoxicity of medicinal products, with considerations for the use of these methods in small and large laboratory animals. The effects of a test substance on the urinary system should first be evaluated using minimally invasive methods. One of these methods is urinalysis. For urinalysis, important considerations include the sampling technique, the volume of the biomaterial, and the turnaround time between urine collection and analysis. Ultrasonography is the most accessible instrumental method in preclinical studies. Ultrasonography can assess organ position, size, structure, and echogenicity and detect abnormalities and changes in real time. Dif ferent method settings are preferred for each species of laboratory animal. Further analysis can include macroscopic examination of organs, measurement of their masses, and microscopic analysis of tissues. Visual assessment should cover the size, colour, and consistency of the ureters, bladder, and kidneys. Nephrotoxicity may manifest as increased apoptosis, vacuolation of renal tubular epithelial cells, epithelial degeneration or dystrophy, oedema, diapedesis-associated haemorrhages, acute tubular and papillary necrosis, necrosis of the Bowman–Schumlansky capsule, casts and crystals in the tubular lumen, glomerulopathy with the corresponding changes, and inflammatory and vascular reactions.CONCLUSIONS. The study analysed and systematised instrumental and laboratory methods for assessing the functional state of the urinary system in preclinical studies. The authors outlined the basic principles for a structured and comprehensive study of the potential nephrotoxicity of novel medicines. The assessment of nephrotoxicity should start with simple and minimally invasive laboratory and instrumental methods, which include general urinalysis and microscopic examination of urine sediment. These methods can detect organ dysfunction that has not yet presented with an associated anatomical lesion. A more in-depth analysis should involve histological and immunohistochemical methods to examine the urinary tissues of laboratory animals.
Optimisation of a Cytogenetic Sample Preparation Procedure for In Vivo Metaphase Analysis of Mammalian Bone Marrow Cells
Authors: Verner A. O. , Ustinova T. M. , Vengerovich N. G. 
Published: July 2024

INTRODUCTION. Metaphase analysis is used to assess the mutagenicity of medicines, and its accuracy depends directly on the quality of cytogenetic preparations. Existing procedures for cytogenetic sample preparation require optimisation because of their time and labour intensiveness and potential for artefactual chromosome loss that leads to errors in the interpretation of analytical results.AIM. This study aimed to optimise a cytogenetic sample preparation procedure for mammalian bone marrow cells for in vivo metaphase analysis.MATERIALS AND METHODS. The study was performed in randomly bred male mice weighing 18–20 g. The authors prepared cytogenetic samples of bone marrow cells according to the procedures set forth in the Guidelines for Conducting Preclinical Studies of Drugs (A.N. Mironov (ed.), 2012), and GOST 34659-2020, Methods for Testing the Impact of Chemical Products on the Human Body. Assessment of Chromosomal Aberrations in Bone Marrow Cells of Mammals, as described and with modifications. Sample preparation involved using Hanks’ solution, sodium citrate and potassium chloride solutions, acetic acid, methyl alcohol, medium 199, HEPES, and Giemsa stain.RESULTS. The authors conducted an experimental comparison of the characteristics of cytogenetic preparations of mammalian bone marrow cells for in vivo metaphase analysis prepared using several methods. The samples prepared in accordance with the Guidelines for Conducting Preclinical Studies of Drugs exhibited the highest percentage of analysable metaphase plates, in comparison with those obtained using the other sample preparation procedures. The authors optimised the sample prepara tion conditions, including the composition of the hypotonic solution (0.56% KCl), the time of hypotonic treatment (20 min), the time of fixation (12 h), and the composition of the culture medium (liquid medium 199 with Hanks’ salts and glutamine). The authors introduced a step of preliminary chromosome fixation with 6% acetic acid, which generally contributed to an increase in the proportion of analysable metaphase plates by reducing the scattering and sticking of chromosomes. CONCLUSIONS. The authors presented a modified sample preparation procedure for cytogenetic preparations that, compared with the previous procedures, offers an increase in the percentage of analysable metaphase plates to 56%, a 12% rise in the percentage of full sets of chromosomes (n=40), and a 2–10-hour reduction in the preparation time.
Pharmacokinetics Study of a New Isoxazole Derivative in Rats Using HPLC-MS/MS for Blood Sample Analysis
Authors: Yaichkov I. I. , Khokhlov A. L. , Korsakov M. K. , Shetnev A. A. , Volkhin N. N. , Petukhov S. S. 
Published: July 2024

INTRODUCTION. Systemic exposure studies of a selective carbonic anhydrase II inhibitor, the isoxazole derivative 5-[5-(trifluoromethyl)-1,2-oxazole-3-yl]-furan-2-sulfonamide (TFISA), require evaluating its pharmacokinetics in whole blood because the compound can accumulate in erythrocytes. Currently, no bioanalytical procedures have been developed to achieve this.AIM. This study aimed to develop a bioanalytical procedure for the determination of TFISA and its metabolites (N-hydroxy-5-[5-(trifluoromethyl)-1,2-oxazole-3-yl]-furan-2-sulfonamide and N-acetyl-5-[5-(trifluoromethyl)-1,2-oxazole-3-yl]-furan-2-sulfonamide) in the blood of laboratory animals and compare the pharmacokinetics of TFISA ophthalmic suspension in rats after a single ocular or intraperitoneal administration.MATERIALS AND METHODS. The quantitative determination was performed by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS) using rat and rabbit blood samples. The chromatographic separation used a Zorbax Eclipse Plus C18 column (150×3.0 mm, 3.5 µm) and a gradient elution system of 0.1% aqueous formic acid and methanol. The multiple reaction monitoring mass spectrometry mode was used for detection. The pharmacokinetics study was conducted in 2 groups of 6 Wistar rats (3 males and 3 females per group). Group 1 received an instillation of 1% TFISA ophthalmic suspension in each eye at a dose of 3.7 mg/kg. Group 2 received an intraperitoneal injection of the same product at the same dose. Blood samples were collected at baseline and at several intervals after administration.RESULTS. The authors developed a bioanalytical procedure for the determination of TFISA and its metabolites in the blood of laboratory animals (rabbits and rats). This HPLC-MS/MS procedure was fully validated in accordance with the requirements of the EAEU legislation and the ICH M10 guideline. The analytical ranges in blood were 20–20000 for TFISA, 2–2000 for the N-hydroxy metabolite, and 0.1–100.0 ng/mL for the N-acetyl metabolite. The maximum blood levels after ocular instillation (mean±SD) were 8173±1491 for TFISA, 694±271 for the N-hydroxy metabolite, and 6.33±1.51 ng/mL for the N-acetyl metabolite. The half-lives for this route of administration were 58±10 (TFISA), 70±24 (N-hydroxy metabolite), and 14±3 h (N-acetyl metabolite). The bioavailability of TFISA was 90.18%. CONCLUSIONS. The developed and validated bioanalytical procedure for the determination of TFISA and its metabolites in the blood of laboratory animals has been successfully applied to samples of rat whole blood. According to the study of ophthalmic suspension pharmacokinetics, TFISA and its metabolites have long halflives and high bioavailability.
Programme for Studying Medicinal Products for Generalised Anxiety Disorder: Analysis of the European Medicines Agency Guideline
Authors: Solovyova A. P. , Surmilo I. M. 
Published: July 2024

INTRODUCTION. Generalised anxiety disorder (GAD) is the least studied anxiety disorder, as patients present with comorbid mood disorders. Finding effective treatment methods for GAD is of the utmost importance; therefore, it is essential to develop novel medicinal products for GAD. Proper clinical programme design is key to obtaining reliable data on the effectiveness and safety of a medicinal product. Currently, the Russian Federation lacks methodological guidelines for clinical studies of these medcinal products, and there is a need for developing such guidelines.AIM. This review aimed to assess the possibility of applying the methodological approaches described in international guidelines to Russian clinical trials to develop medicinal products for GAD.DISCUSSION. Having analysed the main provisions of the Guideline on the clinical investigation of medicinal products indicated for generalised anxiety disorder by the European Medicines Agency (EMA), the authors of this review outlined the main stages of clinical development and the methodology for using clinical data to evaluate the safety and efficacy of medicinal products for GAD. Clinical development programmes for these medicinal products should take into account research staging and mandatory long-term safety and additive effect assessments. This review highlights aspects of selecting the design, population, and primary and secondary endpoints for a clinical trial. Particular attention is paid to the consideration of comorbidities in patients.CONCLUSION. The provisions set forth in the EMA guideline can inform the development of national guidelines for studying medicinal products for GAD.
ENGLISH DEPARTMENT STUDENTS’ PERCEPTIONS OF FLIPPED CLASSROOM IMPLEMENTATION IN SEMANTIC CLASS
Authors: Farida Farida , Purwanti Indah Tri , Eliwarti Eliwarti 
Published: July 2024

This research aims to examine the influence of the use of learning media on students’ learning motivation and learning outcomes in creative economics courses at an education department of one university in Indonesia. The research was carried out employing a quantitative approach with descriptive research methods. The population of this research is undergraduate students from three classes (2019 -2021 cohorts) at this department with a total population of 232 students. They were selected using the Simple Random Sampling method so that the number of samples taken was 70 students. Data were collected using a questionnaire which was distributed to respondents via the Google Form. Data analysis was carried out using regression analysis with the path analysis method to test the research hypothesis. This study found that: 1) the use of learning media has a positive effect on students’ learning motivation; 2) learning motivation has a positive effect on students’ learning outcomes; 3) the use of learning media has a positive effect on students’ learning outcomes (with learning motivation as a mediator). These findings imply that the use of learning media is highly recommended for improving students’ learning outcomes. This influence becomes greater when learning motivation is used as a mediator. This study also shows that learning motivation acts as an intermediary between the use of learning media and students’ outcomes.