bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone Histomorphometry of Transiliac Paired Bone Biopsies After 6 or 12 Months of Treatment With Oral Strontium Ranelate in 387 Osteoporotic Women: Randomized Comparison to Alendronate
Authors: Chavassieux Pascale , Meunier Pierre J , Roux Jean Paul , Portero-Muzy Nathalie , Pierre Marlène , Chapurlat Roland 
Published: August 2013

ABSTRACT

Preclinical studies indicate that strontium ranelate (SrRan) induces opposite effects on bone osteoblasts and osteoclasts, suggesting that SrRan may have a dual action on both formation and resorption. By contrast, alendronate (ALN) is a potent antiresorptive agent. In this multicenter, international, double-blind, controlled study conducted in 387 postmenopausal women with osteoporosis, transiliac bone biopsies were performed at baseline and after 6 or 12 months of treatment with either SrRan 2 g per day (n = 256) or alendronate 70 mg per week (n = 131). No deleterious effect on mineralization of SrRan or ALN was observed. In the intention-to-treat (ITT) population (268 patients with paired biopsy specimens), changes in static and dynamic bone formation parameters were always significantly higher with ALN compared with SrRan at month 6 (M6) and month 12 (M12). Static parameters of formation were maintained between baseline and the last value with SrRan, except for osteoblast surfaces, which decreased at M6. Significant decreases in the dynamic parameters of formation (mineralizing surface, bone formation rate, adjusted apposition rate, activation frequency) were noted at M6 and M12 in SrRan. Compared with ALN, the bone formation parameters at M6 and M12 were always significantly higher (p &lt; 0.001) with SrRan. ALN, but not SrRan, decreased resorption parameters. Compared with the baseline paired biopsy specimens, wall thickness was significantly decreased at M6 but not at M12 and cancellous bone structure parameters (trabecular bone volume, trabecular thickness, trabecular number, number of nodes/tissue volume) were significantly decreased at M12 with SrRan; none of these changes were significantly different from ALN. In conclusion, this large controlled paired biopsy study over 1 year shows that the bone formation remains higher with a lower diminution of the bone remodeling with SrRan versus ALN. From these results, SrRan did not show a significant anabolic action on bone remodeling. © 2014 American Society for Bone and Mineral Research.
Malignant Mesothelioma: Development to Therapy
Authors: Thompson Joyce K. , Westbom Catherine M. , Shukla Arti 
Published: August 2013

ABSTRACTMalignant mesothelioma (MM) is an aggressive cancer of the mesothelium caused by asbestos. Asbestos use has been reduced but not completely stopped. In addition, natural or man‐made disasters will continue to dislodge asbestos from old buildings into the atmosphere and as long as respirable asbestos is available, MM will continue to be a threat. Due to the long latency period of MM development, it would still take decades to eradicate this disease if asbestos was completely removed from our lives today. Therefore, there is a need for researchers and clinicians to work together to understand this deadly disease and find a solution for early diagnosis and treatment. This article focuses on developmental mechanisms as well as current therapies available for MM. J. Cell. Biochem. 115: 1–7, 2014. © 2013 Wiley Periodicals, Inc.
Influence of fludarabine on the pharmacokinetics of oral busulfan during pretransplant conditioning for hematopoietic stem cell transplantation
Authors: de Castro Francine Attié , Lanchote Vera Lucia , Voltarelli Julio Cesar , Colturato Virgílio Antônio Rensi , Simões Belinda Pinto 
Published: August 2013

AbstractThis study evaluated the influence of fludarabine on the pharmacokinetics of busulfan administered orally to patients receiving a conditioning regimen for hematopoietic allogeneic stem cell transplantation (HSCT). Twenty‐six patients treated with oral busulfan (1 mg/kg/6 h for 4 days) were divided into two groups according to the concomitant administration of fludarabine (n = 11; 30 mg/m2 for 5 days) or subsequent administration of cyclophosphamide (n = 15; 60 mg/kg for 2 days). Serial blood samples were collected on Day 4 of busulfan administration. Plasma busulfan concentrations were determined by HPLC‐UV and the pharmacokinetic parameters were calculated using the WinNonlin program. Patients concomitantly treated with fludarabine showed reduced apparent clearance of busulfan (110.5 mL/h/kg vs. 157.4 mL/h/kg) and higher AUC0–6 (area under the plasma concentrations vs. time curve) than patients subsequently treated with cyclophosphamide (7.9 µg h/mL vs. 5.7 µg h/mL). No association was observed between busulfan AUC0–6 and clinical evolution of the patients. Although plasma busulfan concentrations were higher in patients receiving concomitant fludarabine, myelosuppression‐related toxicity was less frequent than in patients treated with busulfan and cyclophosphamide. The results suggest that patients treated with fludarabine should receive 30% lower busulfan doses during conditioning protocols for HSCT.
Two Questions of Erdős on Hypergraphs above the Turán Threshold
Authors: Markström Klas 
Published: August 2013

AbstractFor ordinary graphs it is known that any graph G with more edges than the Turán number of must contain several copies of , and a copy of , the complete graph on vertices with one missing edge. Erdős asked if the same result is true for , the complete 3‐uniform hypergraph on s vertices. In this note, we show that for small values of n, the number of vertices in G, the answer is negative for . For the second property, that of containing a , we show that for the answer is negative for all large n as well, by proving that the Turán density of is greater than that of .
Virological diagnosis and management of two cases of congenital measles
Authors: Giusti Delphine , Burette Julie , Nguyen Yohan , Lévêque Nicolas , Graesslin Olivier , Andreoletti Laurent 
Published: August 2013

AbstractMeasles is a highly contagious viral infection causing congenital infections with a risk of neurological complications in the newborn. Two cases of measles, which occurred in pregnant women within 14 days before the delivery, are described. Mother‐to‐child transmission of the virus was documented in the newborns either by RT‐PCR in saliva or by IgM detection in blood. The measles strains evidenced in saliva samples were genotyped and belonged to the D4 Genotype. An early viral RT‐PCR detection allowed successful immunoglobulin prophylaxis in one newborn taking into account that the duration between the onset of the skin rash in the mother and the delivery was less than 6 days. Twenty‐four months later, none of the newborns developed classical or neurological clinical signs of measles infection. Measles RT‐PCR assay in salivary samples can be used before symptoms develop in the infant to confirm early mother‐to‐child transmission, therefore permitting the use of an immunoglobulin prophylaxis in the newborn. J. Med. Virol. 85:2136–2138, 2013. © 2013 Wiley Periodicals, Inc.
Detection of high‐risk human papillomaviruses in fresh breast cancer samples using the hybrid capture 2 assay
Authors: Liang Weili , Wang Jianli , Wang Chongjie , Lv Yanrong , Gao Haidong , Zhang Kai , Liu Huantao , Feng Jinbo , Wang Leiyi , Ma Rong 
Published: August 2013

AbstractThe etiology of breast cancer remains unknown and the role of human papillomavirus (HPV) in breast carcinogenesis is controversial. This study investigated the prevalence of high‐risk HPV infections in Chinese women with breast cancer and the possible relationship between high‐risk HPV infection and the clinicopathological characteristics of breast cancer. Tumor cells from 224 fresh breast cancer samples and 37 fresh breast fibroadenomas were collected for hybrid capture 2 (HC2) assay. HC2 was the only technique approved by the United States Food and Drug Administration for screening for high‐risk HPV infection in 2008. The prevalence of high‐risk HPV infection in breast cancer samples was 21.4%, which was slightly higher than the 16.2% observed in breast fibroadenomas. Age and menopausal status were not risk factors for high‐risk HPV infection among breast cancer patients. The clinical and pathological characteristics of breast cancer showed no significant correlation with high‐risk HPV infection. Although the prevalence of 13 subtypes of high‐risk HPV infections was similar in breast cancer and nonmalignant breast samples, the presence of high‐risk HPVs in both malignant and benign breast samples implies that a possible causal role in breast cancer carcinogenesis could not be ruled out. Clarifying the possible link between high‐risk HPVs and breast cancer might benefit women vaccinated against HPV and could decrease the incidence of HPV‐related breast cancer. J. Med. Virol. 85:2087–2092, 2013. © 2013 Wiley Periodicals, Inc.
Torque teno virus (TTV) in multiple sclerosis patients with different patterns of disease
Authors: Mancuso Roberta , Saresella Marina , Hernis Ambra , Agostini Simone , Piancone Federica , Caputo Domenico , Maggi Fabrizio , Clerici Mario 
Published: August 2013

AbstractTorque teno virus (TTV) is highly prevalent in the general population worldwide. The relationship that TTV establishes with the central nervous system (CNS) of infected hosts is not clear but it is suspected that TTV infection of the CNS lead to increased local expression of inflammatory mediators that may play a role in the pathogenesis of multiple sclerosis (MS). The prevalence and load of TTV in serum and cerebrospinal fluid (CSF) of 207 MS patients and 93 age‐ and sex‐matched healthy controls by qPCR designed on the untranslated region were analyzed. TTV DNA was not detected in CSF, TTV prevalence in serum was similar in MS patients (76.8%) compared to healthy controls (76.3%). Sub analyses performed in MS patients stratified on the basis of clinical phenotypes indicated that TTV viremia was significantly lower in individuals with relapsing remitting compared to chronic progressive disease. Notably, viremia was increased in primary progressive, compared to secondary progressive MS patients, and in relapsing remitting MS patients during quiescent compared to relapsing phases of disease. Since TTV interacts with toll‐like receptor (TLR) stimulating the production of inflammatory cytokines, TLR9 expression were examined, showing that it was augmented on monocytes of chronic progressive MS patients, in whom higher TTV viremia was present, but this did not correlate with a distinct pattern of cytokine production. Overall these findings suggest that, although TTV infects the same proportion of MS patients and healthy controls, the levels of replication of the virus differ among patients, being correlated with the clinical phenotype of disease. J. Med. Virol. 85:2176–2183, 2013. © 2013 Wiley Periodicals, Inc.
Initial viral load in cases of single human papillomavirus 16 or 52 persistent infection is associated with progression of later cytopathological findings in the uterine cervix
Authors: Hamaguchi Daisuke , Miura Kiyonori , Abe Shuhei , Kinoshita Akira , Miura Shoko , Yamasaki Kentaro , Yoshiura Koh‐ichiro , Masuzaki Hideaki 
Published: August 2013

AbstractThe aim of this study was to investigate the relationship between viral load in single human papillomavirus (HPV) 16 or 52 persistent infection and the progression of later cytopathological findings in the uterine cervix. Cervical cytology and HPV genotyping tests were repeated within 3–6 months in 305 women with oncogenic HPV. Twenty‐four cases of single HPV 52 persistent infection and 24 cases of single HPV 16 persistent infection were identified. Cases with later cytopathological findings showing progression were defined as the progression group, while those with no change or regression were the non‐progression group. Relative HPV DNA loads were determined by quantitative real‐time polymerase chain reaction and expressed relative to human albumin (ALB) DNA. Differences between the two groups were evaluated. The median relative HPV 52 DNA load was 2.211 in the progression group and 0.022 in the non‐progression group (Mann–Whitney U‐test, P = 0.003). The median relative HPV 16 DNA load was 4.206 in the progression group and 0.103 in the non‐progression group (P = 0.001). HPV 52 and 16 DNA loads assessed by quantitative real‐time methods may be useful short‐term markers for identifying women at high risk for progression of cervical cytological pathology. J. Med. Virol. 85:2093–2100, 2013. © 2013 Wiley Periodicals, Inc.
Randomized clinical trial to evaluate the efficacy and safety of valganciclovir in a subset of patients with chronic fatigue syndrome
Authors: Montoya Jose G. , Kogelnik Andreas M. , Bhangoo Munveer , Lunn Mitchell R. , Flamand Louis , Merrihew Lindsey E. , Watt Tessa , Kubo Jessica T. , Paik Jane , Desai Manisha 
Published: August 2013

AbstractThere is no known treatment for chronic fatigue syndrome (CFS). Little is known about its pathogenesis. Human herpesvirus 6 (HHV‐6) and Epstein–Barr virus (EBV) have been proposed as infectious triggers. Thirty CFS patients with elevated IgG antibody titers against HHV‐6 and EBV were randomized 2:1 to receive valganciclovir (VGCV) or placebo for 6 months in a double‐blind, placebo‐controlled trial. Clinical endpoints aimed at measuring physical and mental fatigue included the Multidimensional Fatigue Inventory (MFI‐20) and Fatigue Severity Scale (FSS) scores, self‐reported cognitive function, and physician‐determined responder status. Biological endpoints included monocyte and neutrophil counts and cytokine levels. VGCV patients experienced a greater improvement by MFI‐20 at 9 months from baseline compared to placebo patients but this difference was not statistically significant. However, statistically significant differences in trajectories between groups were observed in MFI‐20 mental fatigue subscore (P = 0.039), FSS score (P = 0.006), and cognitive function (P = 0.025). VGCV patients experienced these improvements within the first 3 months and maintained that benefit over the remaining 9 months. Patients in the VGCV arm were 7.4 times more likely to be classified as responders (P = 0.029). In the VGCV arm, monocyte counts decreased (P < 0.001), neutrophil counts increased (P = 0.037) and cytokines were more likely to evolve towards a Th1‐profile (P < 0.001). Viral IgG antibody titers did not differ between arms. VGCV may have clinical benefit in a subset of CFS patients independent of placebo effect, possibly mediated by immunomodulation and/or antiviral effect. Further investigation with longer treatment duration and a larger sample size is warranted. J. Med. Virol. 85:2101–2109, 2013. © 2013 The Authors. Journal of Medical Virology Published by Wiley Periodicals, Inc.
Quantitative methods for estimating foliar uptake of zinc from suspension‐based Zn chemicals
Authors: T Vu Dang , Huang Longbin , V Nguyen Anh , Du Yumei , Xu Zhiping , A Hampton Marc , Li Peng , Rudolph Victor 
Published: August 2013

AbstractThe present study developed methods for quantifying foliar Zn uptake from suspension‐based Zn chemicals of low solubility, which were ZnO (particle size: 0.15–1.34 μm) and a newly synthesized Zn hydroxide nitrate crystal (Zn‐HNC) (50–100 nm thickness and 0.2–1 μm lateral dimension). Recently matured leaves of citrus (Citrus aurantium L. cv. Valencia), capsicum (Capsicum annume L. cv. Giant Bell), and/or tomato (Solanum lycopersicum L. cv. Roma) were in vitro–treated with microdroplets (5 μL per droplet) of Zn‐HNC‐ and ZnO‐suspension solutions on the adaxial surface and incubated under controlled conditions for up to 72 h. Leaf‐washing protocols were compared, including: dilute ethanol (3%), dilute nitric acid (2%), and their combination. The methods for quantifying Zn uptake were: (1) whole‐leaf loading by which droplets of the Zn suspension solutions were loaded onto central regions of both left and right sides of leaf blades and (2) half‐leaf loading by which soluble‐Zn (ZnSO4) droplets were loaded onto only one side of leaf blades while the other was used as the background Zn control. Foliar‐surface characteristics of the plant species affected the effectiveness of the washing methods. The dilute nitric acid (2%; ± 3% ethanol) was required to remove residue particles of the ZnO and Zn‐HNC suspensions from foliar surfaces of capsicum and tomato (highly trichomatic), but the residue Zn chemicals on citrus leaves (nontrichomatic and highly waxy) were similarly and effectively removed by the three washing methods. For quantifying Zn uptake by the leaves, the half‐leaf loading method showed its advantages over the whole‐leaf loading method, because it did not stringently require similar background Zn concentrations in the control and treated leaves at the start and had little risks of secondary absorption of soluble Zn in the washing solution.
Detecting field cancerization using a hyperspectral imaging system
Authors: Neittaanmäki‐Perttu Noora , Grönroos Mari , Tani Taneli , Pölönen Ilkka , Ranki Annamari , Saksela Olli , Snellman Erna 
Published: August 2013

AbstractBackgroundField cancerization denotes subclinical abnormalities in a tissue chronically exposed to UV radiation. These abnormalities can be found surrounding the clinically visible actinic keratoses.ObjectivesThe aim of this study was to test the feasibility of a hyperspectral imaging system in the detection of multiple clinical and subclinical AKs for early treatment of the affected areas.Materials and MethodsAltogether 52 clinical AKs in 12 patients were included in this study. In six patients digital photos were taken of the naive AKs, and again after methylaminolevulinate(MAL)‐fluorescence diagnosis which was used to teach HIS to find subclinical lesions. After 2–3 days when the MAL had vanished, the hyperspectral images were taken. Biopsies were taken from clinical AKs, healthy‐looking skin and several suspected subclinical AKs. In the other six patients digital and hyperspectral images were taken of the naive AKs followed by one biopsy per patient.ResultsHIS detected all clinically visible 52 AKs and numerous subclinical lesions. The histopathology of the 33 biopsied lesions were concordant with the HIS results showing either AK (n = 28) or photodamage (n = 5). Of the 28 histopathologically confirmed AKs, 16 were subclinical. A specific diffuse reflectance spectrum of an AK and healthy skin was defined.ConclusionThe hyperspectral imaging system offers a new, non‐invasive method for early detection of field cancerization. Lasers Surg. Med. 45:410–417, 2013. © 2013 Wiley Periodicals, Inc.
Development of a ta‐C diamond‐like carbon (DLC) coating by magnetron sputtering for use in precision glass molding
Authors: Bernhardt F. , Georgiadis K. , Dolle L. , Dambon O. , Klocke F. 
Published: August 2013

AbstractDiamond‐like carbon (DLC) coatings have become the most versatile class of coatings in various applications. However, most methods are not practical for industrial fabrication chains. The work presented here the deposition of ta‐C (tetrahedral amorphous carbon = ta‐C) coatings on molding tools for use in precision glass molding by Magnetron Sputtering Ion Plating (MSIP). The coating properties are analyzed by Scanning Electron Microscopy (SEM), Raman spectroscopy and molding tests. By using ion plating, carbon bonds can be induced to change towards the sp3 form by increasing the internal coating stress. Using this technique, it was possible to increase the sp3 ratio to about 60%, whereas 40% or less is often reported for conventional sputtering.
Development and qualification of a MSIP PVD iridium coating for precision glass moulding
Authors: Bobzin K. , Bagcivan N. , Brögelmann T. , Münstermann T. 
Published: August 2013

AbstractThe replicative production technology precision glass moulding becomes more important due to the increasing demand on complex optical lenses. A noble metal coating system deposited by MSIP‐PVD is used to protect the functional surface of the moulding die to increase tool lifetime and surface quality. The objective of this report is to characterise and qualify a pure iridium coating for the suitability as protection coating against glass sticking and wear in contact with different glasses. The coating was developed concerning mechanical properties and tested in a heating experiment in contact with six glasses. It was possible to deposit a metallic iridium coating with a hardness of (26.5 ± 3.6) GPa by verifying deposition temperature and gas contribution. It could be proved, that no visible interaction between coating and different low‐Tg glasses happened. Because of the high mechanical hardness and low reactivity with different low‐Tg glasses an iridium coating is a promising protection coating for precision glass moulding application.
Nano‐impact test on PVD‐coatings with graded mechanical properties for assessing their brittleness
Authors: Bouzakis K.‐D. , Skordaris G. , Gerardis S. , Bouzakis E. 
Published: August 2013

AbstractIn the described investigations, nano‐impact tests were conducted on PVD films with graded mechanical properties. The properties gradation was induced by micro‐blasting at different pressures for constant duration. Micro‐blasting improves the film mechanical properties due to the induced residual compressive stresses into the coating structure. The film mechanical properties as well as the ratio of the film yield to rupture stress (SY/SM) grow up to a certain micro‐blasting pressure. The augmentation of SY/SM ratio increases simultaneously the film brittleness. For analyzing the effect of the SY/SM ratio on the film brittleness, a multi‐layer 3D‐FEM model simulating the nano‐impact test was developed employing the LS‐DYNA software package. The FEM calculated results converge sufficiently with the attained experimental ones. Based on these results, the film's failure initiation and evolution can be predicted depending on the SY/SM ratio of the individual film layers.
Coated tools wear prediction in milling explained by the strain rate affected film fatigue failure
Authors: Bouzakis K.‐D. , Paraskevopoulou R. , Makrimallakis S. , Katirtzoglou G. , Bouzakis E. , Efstathiou K. 
Published: August 2013

AbstractThe knowledge of coated tool wear mechanisms in milling is central for explaining the film failure and selecting the appropriate cutting strategy and conditions. The described tool wear investigations in up and down milling were carried out at various cutting conditions, by coated cemented carbide inserts fixed on tool holders of different diameters. The variable stress, strain and strain rate fields developed in the tool during cutting affect the film‐substrate deformation and in this way the resulting coating's loads and its fatigue failure. The effective tool life, up to a certain flank wear width was experimentally determined and correlated to the cutting edge entry impact duration (CEEID) into the workpiece material. Using a commercial CAD/CAM system, the geometry of the chip and of the contact between the cutting edge and workpiece was calculated in various milling cases and the corresponding tool wear evolution predicted. The latter converges with cutting edge entry impact duration and with the associated film fatigue critical force, affected by the coating strain rate.
Transmission electron microscope (TEM) study of annealed gamma alumina coatings for cutting tools and of gamma alumina‐coated cutting tools after the use in cutting tests
Authors: Müller M. G. J. , Mayer J. 
Published: August 2013

AbstractAmong the transition phases of alumina, the gamma alumina‐phase has a high potential for the use as hard coating in many technological applications, because it exhibits excellent properties e. g. high hot hardness and high thermal stability. In the present work, transmission electron microscopic (TEM) studies are used to investigate the phase stability of nanocrystalline gamma alumina‐coatings. These coatings were deposited by means of magnetron sputter ion plating (MSIP) on cutting inserts with a titanium‐aluminum‐nitride‐interlayer or directly onto silicon‐wafers. Before transmission electron microscope‐investigation, the samples were annealed or used in cutting tests.