bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced lignin and cellulose metabolism promote cell wall synthesis and growth of wild soybean HRA under alkali stress
Authors: Hu Yunan , Hu Yongjun , Gao Shujuan , Luan Zhihui , Zhang Tao , Guo Jixun , Shi Lianxuan 
Published: August 2024

Abstract

Background and Aims
Soil salinization adversely threatens plant survival and food production globally. The mobilization of storage reserves in cotyledons and establishment of the hypocotyl/root axis (HRA) structure and function are crucial to the growth of dicotyledonous plants during the post-germination growth period. Here we report the adaptive mechanisms of wild and cultivated soybeans in response to alkali stress in soil during the post-germination growth period.

Methods
Differences in physiological parameters, microstructure, and the types, amounts and metabolic pathways of small-molecule metabolites and gene expression were compared and multi-omics integration analysis was performed between wild and cultivated soybean under sufficient and artificially simulated alkali stress during the post-germination growth period in this study.

Key Results
Structural analysis showed that the cell wall thickness of wild soybean under alkali stress increased, whereas cultivated soybeans were severely damaged. A comprehensive analysis of small-molecule metabolites and gene expression revealed that protein breakdown in wild soybean cotyledons under alkali stress was enhanced, and transport of amino acids and sucrose increased. Additionally, lignin and cellulose syntheses in wild soybean HRA under alkali stress were enhanced.

Conclusions
Overall, protein decomposition and transport of amino acids and sucrose increased in wild soybean cotyledons under alkali stress, which in turn promoted HRA growth. Similarly, alkali stress enhanced lignin and cellulose synthesis in the wild soybean HRA, which subsequently enhanced cell wall synthesis, thereby maintaining the stability and functionality of the HRA under alkali stress. This study presents important practical implications for the utilization of wild plant resources and sustainable development of agriculture.
Automatic detection of fish scale circuli using deep learning
Authors: Hanson Nora N , Ounsley James P , Henry Jason , Terzić Kasim , Caneco Bruno 
Published: August 2024

Abstract
Teleost fish scales form distinct growth rings deposited in proportion to somatic growth in length, and are routinely used in fish ageing and growth analyses. Extraction of incremental growth data from scales is labour intensive. We present a fully automated method to retrieve this data from fish scale images using Convolutional Neural Networks (CNNs). Our pipeline of two CNNs automatically detects the centre of the scale and individual growth rings (circuli) along multiple radial transect emanating from the centre. The focus detector was trained on 725 scale images and achieved an average precision of 99%; the circuli detector was trained on 40 678 circuli annotations and achieved an average precision of 95.1%. Circuli detections were made with less confidence in the freshwater zone of the scale image where the growth bands are most narrowly spaced. However, the performance of the circuli detector was similar to that of another human labeller, highlighting the inherent ambiguity of the labelling process. The system predicts the location of scale growth rings rapidly and with high accuracy, enabling the calculation of spacings and thereby growth inferences from salmon scales. The success of our method suggests its potential for expansion to other species.
An introduction to key ecological concepts, financial opportunities, and risks underpinning aspirations for nature positive
Authors: Luxton Sarah J , Smith Greg S , Williams Kristen J , Ferrier Simon , Bond Anthelia J , Prober Suzanne M 
Published: August 2024

Abstract
Global biodiversity is in decline, and businesses and society are being required to urgently create new operating models to ameliorate the crisis. Among the strategies proposed to do this, implementing the concept of nature positive has captured worldwide attention. Critical to its success will be effective collaboration between ecologists and businesspeople, driven by a shared understanding of key nature positive terminology, concepts, and risks. To this end, we introduce three core aspects: the ecological concepts in the definition of nature positive (health, abundance, diversity, and resilience), a typology of financial instruments that may be applied to achieving nature positive, and an overview of risks to biodiversity and society. The pivotal findings include that ecological complexity and uncertainty belie the simplicity of the definition of nature positive and that managing risk requires embedding aspirations into existing and emerging biodiversity conservation and restoration science and policy. Although it is challenging, nature positive deserves pursuit.
Assessing the breadth and multidisciplinarity of the conservation curriculum in the United Kingdom and Australia
Authors: Slater Helena , Fisher Janet , Holmes George , Sandbrook Chris , Keane Aidan 
Published: August 2024

Abstract
There have been repeated calls to train conservationists capable of transcending disciplinary boundaries. However, little empirical work has been done to document conservation teaching. We investigate the content taught in conservation higher education across the United Kingdom and Australia. Using data from an online survey and content analysis of module descriptions, we assess the prevalence of subject areas in 146 conservation modules and topics in 368 conservation modules and 62 conservation degrees. Biological sciences subject areas were represented in 92% of the modules, whereas social sciences subject areas only featured in 60% and humanities in 24%. Of the modules teaching biological sciences subject areas, 84% included biological sciences faculty but only 31% of the modules covering social sciences subject areas included faculty from the social sciences. Who teaches matters. The disciplinary expertise in conservation education needs to diversify to train conservationists capable of addressing conservation challenges. This requires institutional changes and support from prominent societies to promote interdisciplinary education.
Incorporating a One Health Approach Into the Study of Environmental Crimes and Harms: Towards a ‘One Health Green Criminology’
Authors: Alonso Aitor Ibáñez , South Nigel 
Published: August 2024

Abstract
Amid increasingly intense and frequent Emerging Infectious Disease events, such as COVID-19, it is evident that the current global ecological crisis poses a threat to the health and well-being of humans, non-human animals and ecosystems. This paper aims to expand existing green criminological scholarship by arguing for the incorporation of a ‘One Health’ perspective. This would (1) enable the integration of scientific knowledge to better address threats, harms and crimes to health and well-being; and (2) contribute to the advance of Eco-justice. The study identifies four areas where a One Health perspective within green criminology may reveal overlooked harms and threats to health and well-being. Conversely, integrating green criminology would benefit the One Health scientific community and specific programs.
Definition and Measurement of Violence in the Crime Survey for England and Wales: Implications for the Amount and Gendering of Violence
Authors: Davies Elouise , Obolenskaya Polina , Francis Brian , Blom Niels , Phoenix Jessica , Pullerits Merili , Walby Sylvia 
Published: August 2024

Abstract
The definitions and the methodology used in surveys to measure violence have implications for its estimated volume and gendered distribution. The Crime Survey for England and Wales (CSEW) uses quite a narrow definition of ‘violence against the person’ which excludes crimes which are arguably violent in nature. This article expands the CSEW’s measurement of violence by regarding threats, robbery, sexual violence and mixed violence/property crimes as violence. This results in a shift in the gender distribution of violence, with a higher proportion of violence against women (from 39% to 58%) and by domestic perpetrators (from 29% to 32%). Impacts of violence (injuries and emotional harm) are also affected by the change in definition and disproportionally so for women.
On Special Liberty and the Motivation to Harm
Authors: Kotzé Justin 
Published: August 2024

Abstract
Special liberty is a destructive and criminogenic force operating throughout the social structure and is arguably the main source of social harm today. The subject of special liberty feels entitled to risk harm to others in pursuit of their own expressive and/or instrumental interests. Since its inception, the concept of special liberty has been used to make sense of numerous issues. However, little attention has been directed towards clearly delineating the two main forms of self-interest served by its use. Our understanding of why special liberty is employed in particular circumstances and conditions is therefore limited. This article makes an original contribution by attempting to formalize an initial delineation of expressive and instrumental self-interest apropos the use of special liberty.
Identifying novel risk genes in intracranial aneurysm by integrating human proteomes and genetics
Authors: Wu Congyan , Liu Hanchen , Zuo Qiao , Jiang Aimin , Wang Chuanchuan , Lv Nan , Lin Ruyue , Wang Yonghui , Zong Kang , Wei Yanpeng , Huang Qinghai , Li Qiang , Yang Pengfei , Zhao Rui , Liu Jianmin 
Published: August 2024

Abstract
Genome-wide association studies (GWAS) have become increasingly popular for detecting numerous loci associated with intracranial aneurysm (IA), but how these loci function remains unclear. In this study, we employed an integrative analytical pipeline to efficiently transform genetic associations and identify novel genes for IA.
Using multidimensional high-throughput data, we integrated proteome-wide association studies (PWAS), transcriptome-wide association studies (TWAS), Mendelian randomization (MR) and Bayesian co-localization analyses to prioritize genes that can increase IA risk by altering their expression and protein abundances in the brain and blood. Moreover, single-cell RNA sequencing (scRNA-seq) of the circle of Willis was performed to enrich filtered genes in cells, and gene set enrichment analysis (GSEA) was conducted for each gene using bulk RNA-seq data for IA.
No significant genes with cis-regulated plasma protein levels were proven to be associated with IA. The protein abundances of five genes in the brain were found to be associated with IA. According to cellular enrichment analysis, these five genes were expressed mainly in the endothelium, fibroblasts and vascular smooth muscle cells. Only three genes, CNNM2, GPRIN3 and UFL1, passed MR and Bayesian co-localization analyses. While UFL1 was not validated in confirmation PWAS as it was not profiled, it was validated in TWAS. GSEA suggested these three genes are associated with the cell cycle. In addition, the protein abundance of CNNM2 was found to be associated with IA rupture (based on PWAS, MR and co-localization analyses).
Our findings indicated that CNNM2, GPRIN3 and UFL1 (CNNM2 correlated with IA rupture) are potential IA risk genes that may provide a broad hint for future research on possible mechanisms and therapeutic targets for IA.
Cardiovascular, renal and mortality risk by the KDIGO heatmap in Japan
Authors: Maruyama Shoichi , Tanaka Tetsuhiro , Akiyama Hiroki , Hoshino Mitsuru , Inokuchi Shoichiro , Kaneko Shuji , Shimamoto Koji , Ozaki Asuka 
Published: August 2024

Abstract

Background
This study aimed to assess the prognosis of people with chronic kidney disease (CKD) in Japan using the Kidney Disease Improving Global Outcomes (KDIGO) heatmap.

Methods
The prognoses of individuals with estimated glomerular filtration rates (eGFR) &lt; 90 ml/min/1.73 m2 were evaluated based on the KDIGO heatmap using an electronic medical record database in Japan. The primary outcome was major adverse cardiovascular events (MACE), a composite of myocardial infarction (MI), stroke, heart failure (HF) hospitalization, and in-hospital death (referred to as MACE1). Additionally, ad hoc MACE2 (MI hospitalization, stroke hospitalization, HF hospitalization, and in-hospital death) was examined. The secondary outcome was the renal outcome.

Results
Of the 543 606 individuals included, the mean age was 61.6 ± 15.3 years, 50.1% were male, and 40.9% lacked urine protein results. The risk of MACEs increased independently with both eGFR decline and increasing proteinuria from the early KDIGO stages: Hazard ratios (with 95% confidence interval) of MACE1 and MACE2, compared to G2A1 were 1.16 (1.12–1.20) and 1.17 (1.11–1.23), respectively, for G3aA1, and 1.17 (1.12–1.21) and 1.35 (1.28–1.43), respectively, for G2A2. This increased up to 2.83 (2.54–3.15) and 3.43 (3.00–3.93), respectively, for G5A3. Risks of renal outcomes also increased with CKD progression.

Conclusions
This study is the first to demonstrate the applicability of the KDIGO heatmap in assessing cardiovascular and renal risk in Japan. The risk increased from the early stages of CKD, indicating the importance of early diagnosis and intervention through appropriate testing.
Trading Stocks Builds Financial Confidence and Compresses the Gender Gap
Authors: Jha Saumitra , Shayo Moses 
Published: August 2024

Abstract
Many studies document low rates of financial literacy and suboptimal levels of participation in financial markets. These issues are particularly acute among women. Does this reflect a self-reinforcing trap? If so, can a nudge to participate in financial markets generate knowledge, confidence and further increase informed participation? We conduct a large field experiment that enables and incentivises working-age men and women—a challenging group to reach with standard financial training programs—to trade stocks for four to seven weeks. We provide no additional educational content. We find that trading significantly improves financial confidence, as reflected in stock market participation, objective and subjective measures of financial knowledge, and risk tolerance. These effects are especially strong among women. Participants also become more self-reliant and consult others less when making financial decisions.
Shared care for patients with a left ventricular assist device: a scoping review
Authors: Drost Valérie C E , Otterspoor Luuk C , Zijderhand Casper F , Sjatskig Jelena , Kluin Jolanda , De Boer Rudolf A , Caliskan Kadir 
Published: August 2024

Abstract
Left ventricular assist devices (LVADs) are increasingly implanted in patients with advanced heart failure. Currently, LVAD care is predominantly concentrated at specialized tertiary care hospitals. However, the increasing workload and logistical burden for implanting centres pose significant challenges to accessing care for individual patients in remote areas. An emerging approach to LVAD patient management is the use of a shared care model (SCM), which facilitates collaboration between implanting centres and local non-implanting hospitals. This scoping review explores and synthesizes the current scientific evidence on the use of SCMs in LVAD care management. Eligible studies were identified in EMBASE, PubMed MEDLINE, Web of Science, Cochrane and Google Scholar. Findings were synthesized in accordance with PRISMA-ScR guidelines. Of the 950 records screened, five articles met the inclusion criteria. Four review articles focused on the proposed benefits and challenges of using SCMs. Main benefits included improved patient satisfaction and continuity of care. Important challenges were initial education of non-implanting centre staff and maintaining competency. One prospective study showed that absence of LVAD-specific care was associated with impaired survival and higher rates of pump thrombosis and LVAD-related infections. The use of SCMs is a promising approach in the long-term management of LVAD patients. However, sufficient evidence about the impact of SCMs on patients and the healthcare system is not currently available. Standardized protocols based on prospective studies are needed to develop safe and effective shared care for LVAD patients.
Social drivers in atrial fibrillation occurrence, screening, treatment, and outcomes: systematic-narrative hybrid review
Authors: Frost Lars , Johnsen Søren Paaske , Benjamin Emelia J , Trinquart Ludovic , Vinter Nicklas 
Published: August 2024

Abstract
The importance of social drivers of health (SDOH) in the occurrence, detection, treatment, and outcome of atrial fibrillation (AF) has attracted increasing attention. Addressing SDOH factors may suggest opportunities to prevent AF and its complications. We aimed to conduct a structured narrative review and summarize current knowledge on the association between race and ethnicity, SDOH, including rural vs. urban habitation, education, income, and neighbourhood, and the risk of AF, its management, and complications. We identified 537 references in PubMed and 473 references in Embase. After removal of duplicates, we screened the abstracts of 975 references, resulting in 113 references that were examined for eligibility. Subsequently, 34 references were excluded leaving 79 references for the review. Evidence of a social gradient in AF incidence and prevelance were conflicting. However, we found substantial evidence indicating social inequities in the detection of AF, access to treatment, and outcomes such as healthcare utilization, bleeding, heart failure, stroke, dementia, work disability, and death. Inequities are reported across various health care systems and constitute a global problem affecting several continents, although data from Africa and South America are lacking. Given the documented social inequities in AF detection, management, and outcomes, there is an urgent need for healthcare systems, policymakers, and society to identify and implement effective interventions that can reduce inequities and improve outcomes in individuals with AF.
The budget impact of implementing atrial fibrillation-screening in European countries
Authors: Eklund Michaela , Bernfort Lars , Appelberg Kajsa , Engler Daniel , Schnabel Renate B , Martinez Carlos , Wallenhorst Christopher , Boriani Giuseppe , Buckley Claire M , Diederichsen Søren Zöga , Svendsen Jesper Hastrup , Montaner Joan , Potpara Tatjana , Levin Lars-Åke , Lyth Johan 
Published: August 2024

Abstract
A budget impact analysis estimates the short-term difference between the cost of the current treatment strategy and a new treatment strategy, in this case to implement population screening for atrial fibrillation (AF). The aim of this study is to estimate the financial impact of implementing population-based AF-screening of 75-year-olds compared with the current setting of no screening from a healthcare payer perspective in eight European countries. The net budget impact of AF-screening was estimated in country-specific settings for Denmark, Germany, Ireland, Italy, Netherlands, Serbia, Spain, and Sweden. Country-specific parameters were used to allow for variations in healthcare systems and to reflect the healthcare sector in the country of interest. Similar results can be seen in all countries AF-screening incurs savings of stroke-related costs since AF treatment reduces the number of strokes. However, the increased number of detected AF and higher drug acquisition will increase the drug costs as well as the costs of physician- and control visits. The net budget impact per invited varied from €10 in Ireland to €122 in the Netherlands. The results showed the increased costs of implementing AF-screening were mainly driven by increased drug costs and screening costs. In conclusion, across Europe, though the initial cost of screening and more frequent use of oral anti-coagulants will increase the healthcare payers’ costs, introducing population screening for AF will result in savings of stroke-related costs.
Atrial fibrillation rhythm management: a matter of timing
Authors: Krisai Philipp , Kühne Michael 
Published: August 2024

Abstract
Rhythm control in patients with atrial fibrillation (AF) has evolved dramatically in the last decades. Several studies have informed us of the benefits of an early rhythm control strategy and primary rhythm control by catheter ablation (CA). Similarly, several studies have investigated the effects of CA in patients with longer AF duration and more comorbidities, especially heart failure. In the current review, we summarize the current evidence on rhythm control at different time points during the disease course of AF [Table 1 and Central illustration].
Screening for atrial fibrillation: the role of CHA2DS2-VASc and atrial fibrillation burden
Authors: Xing Lucas Yixi , Vad Oliver B , Engler Daniel , Svendsen Jesper H , Diederichsen Søren Z 
Published: August 2024

Abstract
Individuals with subclinical atrial fibrillation (AF) face an increased risk of thromboembolic events, which may potentially be mitigated through AF screening and subsequent anticoagulation. However, data from randomized clinical trials (RCTs) indicate a lower stroke risk in subclinical AF compared with the clinical phenotype. This—along with the inherent bleeding risk related to anticoagulation—seems to render the net clinical benefit of AF screening less evident. Further, current guidelines recommend consideration of CHA2DS2-VASc score and AF episode duration to guide screening and treatment. These recommendations, in general, lack support and seem questionable in view of the limited RCT data. More evidence is warranted to provide insights into the potential benefits of screening and treatment of screen-detected AF in specific population subgroups and AF phenotypes.
Incidence and predictors of 2-year mortality following percutaneous left atrial appendage occlusion in the EWOLUTION trial
Authors: Aarnink Errol W , Ince Hueseyin , Kische Stephan , Pokushalov Evgeny , Schmitz Thomas , Schmidt Boris , Gori Tommaso , Meincke Felix , Protopopov Alexey Vladimir , Betts Timothy , Mazzone Patrizio , Grygier Marek , Sievert Horst , De Potter Tom , Vireca Elisa , Stein Kenneth , Bergmann Martin W , Boersma Lucas V A 
Published: August 2024

Abstract

Aims
Sufficient survival time following left atrial appendage occlusion (LAAO) is essential for ensuring the efficacy and cost-effectiveness of this strategy for stroke prevention. Understanding prognostic factors for early mortality after LAAO could optimize patient selection. In the current study, we perform an in-depth analysis of 2-year mortality after LAAO, focusing particularly on potential predictors.

Methods and results
The EWOLUTION registry is a real-world cohort comprising 1020 patients that underwent LAAO. Endpoint definitions were pre-specified, and death was categorized as cardiovascular, non-cardiovascular, or unknown origin. Mortality rates were calculated from Kaplan–Meier estimates. Baseline characteristics significantly associated with death in univariate Cox regression analysis were incorporated into the multivariate analysis. All multivariate predictors were included in a risk model. Two-year mortality rate was 16.4% [confidence interval (CI): 14.0–18.7%], with 50% of patients dying from a non-cardiovascular cause. Multivariate baseline predictors of 2-year mortality included age [hazard ratio (HR) 1.05, CI: 1.03–1.08, per year increase], heart failure (HR 1.73, CI: 1.24–2.41), vascular disease (HR 1.47, CI: 1.05–2.05), valvular disease (HR 1.63, CI: 1.15–2.33), abnormal liver function (HR 1.80, CI: 1.02–3.17), and abnormal renal function (HR 1.58, CI: 1.10–2.27). Mortality rate exhibited a gradual rise as the number of risk factors increased, reaching 46.1% in patients presenting with five or six risk factors.

Conclusion
One in six patients died within 2 years after LAAO. We identified six independent predictors of mortality. When combined, this model showed a gradual increase in mortality rate with a growing number of risk factors, which may guide appropriate patient selection for LAAO.

Clinical trial registration
The original EWOLUTION registry was registered at clinicaltrials.gov under identifier NCT01972282.