bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variation in root traits associated with nutrient foraging among temperate pasture legumes and grasses
Authors: Yang Z. , Culvenor R. A. , Haling R. E. , Stefanski A. , Ryan M. H. , Sandral G. A. , Kidd D. R. , Lambers H. , Simpson R. J. 
Published: September 2015

AbstractTemperate pasture legumes (e.g. Trifolium and Medicago spp.) often have a higher phosphorus (P) requirement for maximum productivity than pasture grasses. This is partly attributed to differences between legumes and grasses in their ability to acquire P from soil. We are the first to report differences in root morphology traits important for soil P acquisition in a range of novel pasture legumes being developed for use in temperate pastures of southern Australia. Up to a 3·6‐fold range in specific root length (SRL) (79–281 m root g−1 root) and 6·1‐fold range in root hair length (RHL) (0·12–0·75 mm) was found between the pasture species. The commonly used Trifolium subterraneum and Medicago sativa had relatively low SRLs and short root hairs, while Ornithopus compressus, O. sativus and Biserrula pelecinus had RHLs and SRLs more similar to those of two grass species that were also assessed. Specific root length was highly correlated with average root diameter, and root traits were relatively stable at different plant ages. We surmise that large differences among pasture legume species in the effective volume of soil explored could translate into significant differences in their critical P requirements (i.e. soil P concentration to achieve 90% of maximum shoot yield).
Unclassified bleeding disorders: outcome of haemostatic challenges following tranexamic acid and/or desmopressin
Authors: Obaji S. , Alikhan R. , Rayment R. , Carter P. , Macartney N. , Collins P. 
Published: September 2015

IntroductionIn a minority of patients with a significant bleeding history no cause is found despite extensive testing and we diagnose such cases as unclassified bleeding disorders (UBD). UBDs may have diverse underlying causes and currently no standard management strategy exists in the event of a haemorrhage or to cover surgery.AimTo document the clinical characteristics and response to treatment of UBDs.MethodsWe performed a retrospective chart review of all patients with UBDs who had an invasive procedure at our centre between 1998 and 2014.ResultsThe commonest symptoms were menorrhagia (89%) and bleeding at the time of surgery (88%) or dental extraction (85%). A total of 33 patients underwent 78 minor and major haemostatic challenges. Haemostatic cover was provided in 28 procedures with tranexamic acid alone, two with desmopressin and 45 with both agents in combination. A successful haemostatic outcome was observed in 70/78 (90%) cases. No patient required additional surgical intervention to achieve haemostasis, but one patient required a platelet transfusion to control postoperative bleeding.ConclusionsThis is the first study to report on the investigation and treatment of UBD. Future studies are needed to further our understanding of the bleeding phenotype and identify any underlying causes.
Clinic and Emergency Room Evaluation and Testing of Headache
Authors: Nye Barbara L. , Ward Thomas N. 
Published: September 2015

BackgroundEvaluation of the headache patient in the outpatient clinic and emergency department (ED) has different focuses and goals. The focus of this paper is to review the evaluation of patients in both settings with mention of evaluation in the pediatric and pregnant patient population. The patient's history should drive the practitioner's decision and evaluation choices. We review recommendations made by the American Board of Internal Medicine and American Headache Society through the Choosing Wisely Campaign, which has an emphasis on choosing the right imaging modality for the clinical situation and elimination/prevention of medication overuse headache, as well as the US Headache Consortium guidelines for migraine headache. We will also review focusing on ED evaluation of the pediatric patient and pregnant patient presenting with headache.ConclusionAt the end of the review we hope to have provided you with a framework to think about the headache patient and what is the appropriate test in the given clinical setting in order to ensure that the patient gets the right diagnosis and is set on a path to the appropriate management plan.
Alice in Wonderland Syndrome, Burning Mouth Syndrome, Cold Stimulus Headache, and HaNDL: Narrative Review
Authors: Valença Marcelo M. , de Oliveira Daniella A. , Martins Hugo André de L. 
Published: September 2015

BackgroundUnusual headache syndromes are not as infrequent in clinical practice as was generally believed. About three fourths of the classified headache disorders found in the ICHD‐II can be considered rare.ObjectiveThe aim of this narrative review was to perform a literature review of the pathophysiology, clinical presentation, diagnostic criteria, and treatment of the following unusual headache disorders: Alice in Wonderland syndrome, burning mouth syndrome, cold stimulus headache, and the syndrome of transient headache and neurologic deficits with cerebrospinal fluid lymphocytosis.MethodA literature review was performed using PubMed for each of the abovementioned headache disorders.ConclusionThe unusual headache syndromes as a distinct group of disorders are not as infrequent in clinical practice as was generally believed. Some of them, albeit considered as unusual, may occur with relative frequency, such as cold stimulus headache and burning mouth syndrome.
Variable asialoglycoprotein receptor 1 expression in liver disease: Implications for therapeutic intervention
Authors: Witzigmann Dominik , Quagliata Luca , Schenk Susanne H. , Quintavalle Cristina , Terracciano Luigi M. , Huwyler Jörg 
Published: September 2015

AbstractAimOne of the most promising strategies for the treatment of liver diseases is targeted drug delivery via the asialoglycoprotein receptor (ASGPR). The success of this approach heavily depends on the ASGPR expression level on parenchymal liver cells. In this study, we assessed the mRNA and protein expression levels of the major receptor subunit, ASGR1, in hepatocytes both in vitro and in vivo.MethodsIn vitro, various liver cancer‐derived cell lines were evaluated. In vivo, we screened the ASGR1 mRNA on 59 hepatocellular carcinoma and matched non‐neoplastic tissue using RNA microarray. In addition, 350 human liver specimens of patients with hepatocellular carcinoma or non‐neoplastic liver diseases were screened for ASGR1 protein level using tissue microarray analysis.ResultsOur data reveal that the ASGR1 mRNA expression directly correlates with the protein level. We demonstrate that the ASGR1 expression is upregulated in cirrhotic specimens and is significantly decreased with increasing hepatocellular carcinoma grade.ConclusionBecause the ASGR1 expression levels are variable between patients, our findings suggest that ASGPR‐based targeting strategies should be combined with ASGPR‐companion diagnostics to maximize clinical benefit.
Influence of malting and lactic acid fermentation on functional bioactive components in cereal‐based raw materials: a review paper
Authors: Hassani Ahmed , Procopio Susanne , Becker Thomas 
Published: September 2015

SummaryDriven by increased health awareness among consumers, the production of foods and beverages enriched with functional bioactive components is gaining more attention. Malting and lactic acid fermentation are biotechnological processes having potential for producing functional foods and beverages. Due to various biochemical and enzymatic induced changes in raw materials, malting of cereal grains and probiotic lactic acid fermentation of plant‐based media increases the nutritional quality of treated raw materials. The improved nutritional quality is attributed to the accumulation of functional bioactive components along with the degradation of anti‐nutritional components. The selection of raw materials and process parameters are important factors to be considered for increasing the functional bioactive components such as dietary fibres, antioxidants and probiotics. This review article reports the current knowledge on the changes of bioactive components during malting and lactic acid fermentation using probiotic bacterial strains. Process parameters which affect the concentration of bioactive components in raw materials will also be described.
An Advanced Visual Qualitative and EVA Green‐Based Quantitative Isothermal Amplification Method to Detect Listeria Monocytogenes
Authors: Cheng Nan , Xu Yuancong , Yan Xinghua , Shang Ying , Zhu Pengyu , Tian Wenying , Liang Zhihong , Xu Wentao 
Published: September 2015

AbstractThe purpose of this study is to apply the visual qualitative (LFD‐LAMP) and advanced quantitative loop‐mediated isothermal amplification (EVA‐LAMP) methods to detect Listeria monocytogenes, which is an important pathogenic foodborne bacterium. This research designed a new set of primers to amplify the hlyA gene of L. monocytogenes. The LFD‐LAMP products were detected by lateral flow device (LFD) with the naked eyes, and the test results were consistent with agarose gel electrophoresis. Meanwhile, an advanced quantitative LAMP assay was established which depended on the EVA Green. The detection limit of the EVA‐LAMP method was 10 pg/μL, and a good linear relationship was obtained. The subsequent analysis of contaminated raw food samples showed that the primers were able to identify L. monocytogenes in food. The LFD‐LAMP and EVA‐LAMP reactions can be used as sensitive, rapid and simple methods for the detection of L. monocytogenes, especially on field detection.Practical ApplicationsThis work suggests a specific, sensitive, cost‐effective and nonpollution LAMP assay for the detection of Listeria monocytogenes. A new set of hlyA‐based primers was designed and successfully used to detect L. monocytogenes in raw food samples. Amplicon detection by visual LFD method proved to be as useful as the gel, but more convenient and clean. The LFD‐LAMP detection method in this study testifies as a significant advancement toward making LAMP a potential on‐site detection. Otherwise, in contrast to the traditional real‐time LAMP approach, this is a novel study to describe a real‐time quantitative LAMP assay based on EVA Green to detect L. monocytogenes in the fields of food safety.
Integrative review of nurse‐led follow‐up after discharge from theICU
Authors: Jónasdóttir Rannveig J. , Klinke Marianne E. , Jónsdóttir Helga 
Published: September 2015

Aim and objectivesTo analyse and synthesise the structure, content, types of outcome variables and advantages of nurse‐led follow‐up of adult patients after discharge from intensive care units.BackgroundFollow‐up service after discharge from the intensive care unit has been suggested as a way of supporting recovery of patients. Nevertheless, varieties exist in the understanding and content of nurse‐led follow‐up.DesignAn integrative review of nurse‐led follow‐up inspired by the framework of Whittemore and Knafl.MethodsAn integrative method merged with the recommendations of thePRISMAstatement was used to structure the review and findings. Online databases PubMed,CINAHL, ScienceDirect and Scopus were searched from the years 2003–2014. The retrieved articles were independently assessed by two reviewers. Critical appraisal was conducted using check lists from Johanna Briggs Institute. Emerging patterns were validated by all the authors throughout the entire process of analysis.ResultsSeventeen papers were included. Three patterns of nurse‐led follow‐up were identified: (1) Ward visits – in the immediate time after discharge from intensive care unit, (2) Ward visits and appointment(s) to an intensive care unit follow‐up clinic and (3) follow‐up visit to an intensive care unit and phone call(s) after discharge. Content of short‐term nurse‐led follow‐up (1) ranged from clinical assessment to supporting patients in articulating their subjective health concerns. Long‐term nurse‐led follow‐up (2, 3) included appointments, phone call(s) or information on where advice could be sought. Types of outcome variables were primarily descriptive. There were strong implications for patients' satisfaction with nurse‐led follow‐up up to six months after discharge.ConclusionNurse‐led follow‐up might promote patients' health and enable use of adequate resources.Relevance to clinical practiceThe findings of this review could be used to design, and test, future interventions and their implementation.
Construction and validation of a scale of assessment of self‐care behaviours anticipatory to creation of arteriovenous fistula
Authors: Sousa Clemente Neves , Figueiredo Maria Henriqueta , Dias Vanessa Filipa , Teles Paulo , Apóstolo João Luís 
Published: September 2015

Aims and objectivesWe developed a scale to assess the self‐care behaviours developed by patients with end‐stage renal disease to preserve the vascular network prior to construction of arteriovenous fistula.BackgroundThe possibility of creation of an arteriovenous fistula depends on the existence of an arterial and venous network in good condition, namely the size and elasticity of the vessels. It is essential to teach the person to develop self‐care behaviours for the preservation of the vascular network, regardless of the modality of dialysis selected.DesignMethodological study.MethodsThe scale was developed based on clinical experience and research conducted by the researcher in the area of the vascular access for haemodialysis. The content of the scale was judged by two panels of experts for content validity. The revised version of the scale was administered to a convenience sample of 90 patients with end‐stage renal disease. In the statistical analysis, we used the Cronbach's alpha, the Kaiser–Meyer–Olkin and scree plot and the principal component analysis with varimax rotation.ResultsA principal component analysis confirmed the univariate structure of the scale (KMO = 0·759, Bartlett's sphericity test‐approximate χ2 142·201, p < 0·000). Cronbach's α is 0·831, varying between 0·711–0·879.ConclusionThis scale revealed properties that allow its use to assess the patients self‐care behaviours regarding the preservation of the vascular network.Relevance to clinical practiceThis scale can be used to evaluate educational programmes for the development of self‐care behaviours in the preservation of vascular network. This scale can identify not only the patients that are able to take care of their vascular network but also the proportion of patients who are not able to do it, that need to be educated.
To ask, or not to ask: the hesitation process described by district nurses encountering women exposed to intimate partner violence
Authors: Sundborg Eva , Törnkvist Lena , Saleh‐Stattin Nouha , Wändell Per , Hylander Ingrid 
Published: September 2015

Aims and objectivesTo improve the understanding of district nurses' experiences of encountering women exposed to intimate partner violence.BackgroundIntimate partner violence is a public health issue that influences all aspects of affected women's lives; it is thus of great relevance to identify intimate partner violence. District nurses in primary health care in Sweden encounter women of all ages and are therefore in a unique position to identify intimate partner violence and provide nursing care. However, studies indicate that several barriers have kept nurses from identifying intimate partner violence. Consequently deeper understanding of district nurses' encounters with women exposed to intimate partner violence is needed.DesignA qualitative study using grounded theory method.MethodsInterviews with 11 district nurses in primary health care in Sweden.ResultsThe hesitation process is central in these encounters. Several barriers to asking and factors that facilitated asking impacted the hesitation process. Under the influence of these factors, district nurses moved from being unaware that identifying intimate partner violence was their professional responsibility, to becoming ambivalent about asking, to starting to prepare themselves to ask about intimate partner violence. The presence of factors that facilitated asking finally made district nurses feel prepared, and then they decided to ask women about intimate partner violence.ConclusionsThe concept of managing the hesitation process could be used to be incorporated in continuing education and training. Barriers and facilitators concerning organisational factors found in this study may be specific to the Swedish health care system, but the concept of the hesitation process is applicable in similar contexts and perhaps to other issues about which it is difficult to ask.Relevance to clinical practiceThis study illuminates the importance of a supportive work environment in reducing district nurses' hesitation to ask about intimate partner violence and to propose continuing education, training and supervision for district nurses regarding intimate partner violence.
Newly graduated nurses’ occupational commitment and its associations with professional competence and work‐related factors
Authors: Numminen Olivia , Leino‐Kilpi Helena , Isoaho Hannu , Meretoja Riitta 
Published: September 2015

Aims and objectivesTo explore newly graduated nurses’ occupational commitment and its associations with their self‐assessed professional competence and other work‐related factors.BackgroundAs a factor affecting nurse turnover, newly graduated nurses’ occupational commitment and its associations with work‐related factors needs exploring to retain adequate workforce. Nurses’ commitment has mainly been studied as organisational commitment, but newly graduated nurses’ occupational commitment and its association with work‐related factors needs further studying.DesignThis study used descriptive, cross‐sectional, correlation design.MethodsA convenience sample of 318 newly graduated nurses in Finland participated responding to an electronic questionnaire. Statistical software, ncss version 9, was used in data analysis. Frequencies, percentages, ranges, means and standard deviations summarised the data. Multivariate Analyses of Variance estimated associations between occupational commitment and work‐related variables. ibm spss Amos version 22 estimated the model fit of Occupational Commitment Scale and Nurse Competence Scale.ResultsNewly graduated nurses’ occupational commitment was good, affective commitment reaching the highest mean score. There was a significant difference between the nurse groups in favour of nurses at higher competence levels in all subscales except in limited alternatives occupational commitment. Multivariate analyses revealed significant associations between subscales of commitment and competence, turnover intentions, job satisfaction, earlier professional education and work sector, competence counting only through affective dimension.ConclusionThe association between occupational commitment and low turnover intentions and satisfaction with nursing occupation was strong. Higher general competence indicated higher overall occupational commitment.Relevance to clinical practiceManagers’ recognition of the influence of all dimensions of occupational commitment in newly graduated nurses’ professional development is important. Follow‐up studies of newly graduated nurses’ commitment, its relationship with quality care, managers’ role in enhancing commitment and evaluation of the impact of interventions on improving commitment need further studying.
Do the levels of tumor makers or proinflammatory cytokines in mid‐trimester cervical fluid predict early‐stage cervical shortening?
Authors: Seong Won Joon , Lee Dong Young , Koo Tae Bon 
Published: September 2015

AbstractAimIn the present study, we aimed to assess the biomarkers in mid‐trimester cervical fluid that can predict early stage cervical shortening.Material and MethodsWe obtained cervical swab specimens from 96 gravidas, after which the cervical length was measured, at approximately 20 weeks of gestation. Cervical length was measured again at 4 weeks after the initial examination. Cervical shortening was noted in 20 women between 20 and 24 weeks of gestation (group A), whereas no cervical shortening was noted in 76 women (group B). We evaluated the use of the levels of tumor markers, proinflammatory cytokines, and matrix metalloproteinase‐8 (MMP‐8) as candidate biomarkers. CA‐125 and carcinoembryonic antigen levels were determined by using an automatic immunoassay system in both groups. Furthermore, IL‐1β, IL‐8, tumor necrosis factor‐α, and MMP‐8 levels were measured using an enzyme‐linked immunosorbent assay.ResultsThe levels of inflammatory cytokines and MMP‐8 did not differ between the two groups, and were not correlated with cervical length or the change in cervical length. Although CA‐125 and carcinoembryonic antigen levels were higher in group A, they were not statistically significant (P = 0.304 and 0.092, respectively).ConclusionEarly stage cervical shortening in mid‐trimester was not associated with an increase in the levels of tumor markers or proinflammatory cytokines in cervical fluid.
Association of matrix metalloproteinase‐10 polymorphisms with susceptibility to pelvic organ prolapse
Authors: Wang Hong , Zhang Zhi‐Qiang , Wang Shu‐Zhen , Lu Jun‐Li , Wang Xiao‐Lan , Zhang Zhen‐Yu 
Published: September 2015

AbstractAimThe aim of this study was to explore the correlation between genetic mutations in matrix metalloproteinase‐10 (MMP‐10) and susceptibility to pelvic organ prolapse (POP).Material and MethodsFrom September 2011 to December 2013, 263 subjects were recruited, including 91 patients with POP (case group) and 172 non‐POP patients (control group). Total MMP‐10 concentrations in serum were measured by enzyme‐linked immunosorbent assay. The genotyping of MMP‐10 was achieved by quantitative real‐time polymerase chain reaction. All data were analyzed with spss 18.0.ResultsWe found that parity, menopause, history of total hysterectomy, and family history of POP were all significantly higher in the POP group than in the control group (P = 0.017, P = 0.046, P = 0.0029 and P < 0.001, respectively). Serum MMP‐10 levels were obviously higher in the POP group than in the control group (P < 0.05). In addition, there was a statistically significant difference between the two groups in the distribution frequency of the MMP‐10 (rs17435959G/C) genotype (P < 0.05). However, the distribution frequency of the MMP‐10 (rs17293607C/T) genotype between the two groups showed no significant differences (P > 0.05). Furthermore, the patients with parity > 2 and postmenopausal women had elevated serum MMP‐10 levels, and the patients with parity > 2 and postmenopausal women who carried the G/C + C/C genotype in the MMP‐10 gene had an increased risk of POP.ConclusionWe support the view that the rs17435959 polymorphism of the MMP‐10 gene may be associated with an increased risk of POP.