bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reserve price and competing bids: Reference points for product evaluations in online auctions?
Authors: Trautmann Stefan T. , van de Kuilen Gijs 
Published: May 2015

AbstractWith an increasing number of products sold in online auctions, the effect of reference points on consumers' product valuations has received much attention in the literature. Two potentially relevant factors affecting bidders' preferences are the sellers' reserve price and the competing bids by other bidders. The current evidence regarding these reference points is mixed. We argue that a focus on studying bids prevents a clear identification of reference point effects. Directly observing product valuations, in two experiments, we find that willingness to pay in online auctions is affected by both reserve prices and competing bids, supporting the constructed values paradigm. Copyright © 2015 John Wiley & Sons, Ltd.
Designed Intercalators for Modification of DNA Origami Surface Properties
Authors: Brglez Josipa , Nikolov Pavel , Angelin Alessandro , Niemeyer Christof M. 
Published: May 2015

AbstractThe modification of the backbone properties of DNA origami nanostructures through noncovalent interactions with designed intercalators, based on acridine derivatized with side chains containing esterified fatty acids or oligo(ethylene glycol) residues is reported. Spectroscopic analyses indicate that these intercalators bind to DNA origami structures. Atomic force microscopy studies reveal that intercalator binding does not affect the structural intactness but leads to altered surface properties of the highly negatively charged nanostructures, as demonstrated by their interaction with solid mica or graphite supports. Moreover, the noncovalent interaction between the intercalators and the origami structures leads to alteration in cellular uptake, as shown by confocal microscopy studies using two different eukaryotic cell lines. Hence, the intercalator approach offers a potential means for tailoring the surface properties of DNA nanostructures.
Influence of Helical Structure on Chiral Recognition of Poly(phenylacetylene)s Bearing Phenylcarbamate Residues of L‐Phenylglycinol and Amide Linage as Pendants
Authors: Zhang Chunhong , Ma Rui , Wang Hailun , Sakai Ryosuke , Satoh Toshifumi , Kakuchi Toyoji , Liu Lijia , Okamoto Yoshio 
Published: May 2015

AbstractFour poly(phenylacetylene)s (PPA-1, PPA-2, PPA-3, PPA-4) bearing phenylcarbamate residues of L‐phenylglycinol and amide linkage as pendants were prepared to be used as chiral stationary phases (CSPs) for high‐performance liquid chromatography (HPLC), and the influences of coating solvents, dimethylformamide (DMF) and tetrahydrofuran (THF), which were used for coating the polymers on silica gel, on the helical structure of the polymers and their chiral recognition abilities were investigated. The structure analysis of PPA-1, PPA-2, PPA-3, PPA-4 by 1H nuclear magnetic resonance (NMR), size exclusion chromatography (SEC), optical rotation, and circular dichroism (CD) spectra indicated that the polymers possess the cis‐transoidal structure with dynamic helical conformation. The polymers in THF seem to have shorter conjugated helical main chains along with a tighter twist conformation than those in DMF. The chiral recognition abilities of PPA-1, PPA-2, PPA-3, PPA-4 with the different helical structures induced by the coating solvents were evaluated as the CSPs in HPLC. The helical structures of PPA-1, PPA-2, PPA-3, PPA-4 induced with THF are preferable for chiral recognition for some racemates compared to those induced with DMF, and higher chiral recognition abilities of PPA-1, PPA-2, PPA-3, PPA-4 were achieved using THF. Chirality 27:500–506, 2015. © 2015 Wiley Periodicals, Inc.
Joint prognostic effect of obesity and chronic systemic inflammation in patients with metastatic colorectal cancer
Authors: Shah Manasi S. , Fogelman David R. , Raghav Kanwal Pratap Singh , Heymach John V. , Tran Hai T. , Jiang Zhi‐Qin , Kopetz Scott , Daniel Carrie R. 
Published: May 2015

BACKGROUNDObesity is strongly linked with chronic systemic inflammation, and each has been linked with disease progression and survival in patients with colorectal cancer (CRC). The authors investigated the joint prognostic effects of obesity and circulating cytokines in patients with metastatic CRC (mCRC), an understudied patient group.METHODSIn 242 chemotherapy‐naive patients with mCRC, the authors measured a multiplex cytokine panel and abstracted clinicopathological features, height, and weight from medical records. Overall survival (OS) was calculated from the date of mCRC diagnosis until the date of death from any cause and evaluated by Kaplan‐Meier analysis and multivariable Cox proportional hazards regression models. Cut points for cytokines were determined by restricted cubic spline regression.RESULTSIn multivariable models, elevated interleukin (IL)−8, IL‐2 receptor alpha, and lactate dehydrogenase (LDH) emerged as significant predictors of poor OS (hazard ratio [HR] and 95% confidence interval [95% CI] for above vs below the (referent) knot point: 2.5 [95% CI, 1.7‐3.7], 1.9 [95% CI, 1.3‐2.7], and 2.2 [95% CI, 1.6‐3.1], respectively; all P<.001). Obesity (body mass index ≥30 kg/m2) was not found to be associated with OS, but appeared to modify the relationships observed with IL‐8 and LDH, which were associated with a significant 4‐fold and 5‐fold risk of death, respectively, in obese patients compared with a 2‐fold risk of death in nonobese patients (P for interaction of .06 and .04, respectively). Similar results emerged from joint effects analysis, in which obese patients with high IL‐8 (or LDH) experienced the highest risk of death.CONCLUSIONSAlthough obesity itself was not found to be independently associated with survival in patients with mCRC, the adverse prognostic significance of LDH and IL‐8 was found to be enhanced in obese patients. Cancer 2015;121:2968–2975. © 2015 American Cancer Society.
Subclassification of prostate cancer circulating tumor cells by nuclear size reveals very small nuclear circulating tumor cells in patients with visceral metastases
Authors: Chen Jie‐Fu , Ho Hao , Lichterman Jake , Lu Yi‐Tsung , Zhang Yang , Garcia Mitch A. , Chen Shang‐Fu , Liang An‐Jou , Hodara Elisabeth , Zhau Haiyen E. , Hou Shuang , Ahmed Rafi S. , Luthringer Daniel J. , Huang Jiaoti , Li Ker‐Chau , Chung Leland W. K. , Ke Zunfu , Tseng Hsian‐Rong , Posadas Edwin M. 
Published: May 2015

BACKGROUNDAlthough enumeration of circulating tumor cells (CTCs) has shown some clinical value, the pool of CTCs contains a mixture of cells that contains additional information that can be extracted. The authors subclassified CTCs by shape features focusing on nuclear size and related this with clinical information.METHODSA total of 148 blood samples were obtained from 57 patients with prostate cancer across the spectrum of metastatic states: no metastasis, nonvisceral metastasis, and visceral metastasis. CTCs captured and enumerated on NanoVelcro Chips (CytoLumina, Los Angeles, Calif) were subjected to pathologic review including nuclear size. The distribution of nuclear size was analyzed using a Gaussian mixture model. Correlations were made between CTC subpopulations and metastatic status.RESULTSStatistical modeling of nuclear size distribution revealed 3 distinct subpopulations: large nuclear CTCs, small nuclear CTCs, and very small nuclear CTCs (vsnCTCs). Small nuclear CTCs and vsnCTC identified those patients with metastatic disease. However, vsnCTC counts alone were found to be elevated in patients with visceral metastases when compared with those without (0.36 ± 0.69 vs 1.95 ± 3.77 cells/mL blood;P<.001). Serial enumeration studies suggested the emergence of vsnCTCs occurred before the detection of visceral metastases.CONCLUSIONSThere are morphologic subsets of CTCs that can be identified by fundamental pathologic approaches, such as nuclear size measurement. The results of this observational study strongly suggest that CTCs contain relevant information regarding disease status. In particular, the detection of vsnCTCs was found to be correlated with the presence of visceral metastases and should be formally explored as a putative blood‐borne biomarker to identify patients at risk of developing this clinical evolution of prostate cancer.Cancer2015;121:3240–3251.©2015 American Cancer Society.
ISAC's Gating‐ML 2.0 data exchange standard for gating description
Authors: Spidlen Josef , Moore Wayne ,   ISAC Data Standards Task Force, Brinkman Ryan R. 
Published: May 2015

AbstractThe lack of software interoperability with respect to gating has traditionally been a bottleneck preventing the use of multiple analytical tools and reproducibility of flow cytometry data analysis by independent parties. To address this issue, ISAC developed Gating‐ML, a computer file format to encode and interchange gates. Gating‐ML 1.5 was adopted and published as an ISAC Candidate Recommendation in 2008. Feedback during the probationary period from implementors, including major commercial software companies, instrument vendors, and the wider community, has led to a streamlined Gating‐ML 2.0. Gating‐ML has been significantly simplified and therefore easier to support by software tools. To aid developers, free, open source reference implementations, compliance tests, and detailed examples are provided to stimulate further commercial adoption. ISAC has approved Gating‐ML as a standard ready for deployment in the public domain and encourages its support within the community as it is at a mature stage of development having undergone extensive review and testing, under both theoretical and practical conditions. © 2015 International Society for Advancement of Cytometry
From specialist to generalist: Developmental transformations in the genetic structure of early child abilities
Authors: Cheung Amanda K. , Harden K. Paige , Tucker‐Drob Elliot M. 
Published: May 2015

ABSTRACTThe heritability of abilities increases substantially over development, and much of heritable variation in abilities is shared with other abilities. No study, however, has formally tested the extent to which developmental increases in heritability occur on shared versus unique variation in child abilities. A transactional perspective predicts that the relative proportion of shared to total genetic variance will increase with age, whereas an endogenous perspective predicts that such proportion will be invariant with age. We tested these competing predictions using data from a sample of 292 twins providing a total of 578 cross‐sectional and longitudinal observations between ages 0 and 6 years on measures of Communication, Gross Motor, Fine Motor, Problem‐Solving, and Personal‐Social abilities. Consistent with predictions of the transactional perspective, developmental increases in heritability were localized to variance shared across abilities. © 2015 Wiley Periodicals, Inc. Dev Psychobiol 57: 566–583, 2015.
Coregulation of respiratory sinus arrhythmia between parents and preschoolers: differences by children's externalizing problems
Authors: Lunkenheimer Erika , Tiberio Stacey S. , Buss Kristin A. , Lucas‐Thompson Rachel G. , Boker Steven M. , Timpe Zachary C. 
Published: May 2015

ABSTRACTThe coordination of physiological processes between parents and infants is thought to support behaviors critical for infant adaptation, but we know little about parent‐child physiological coregulation during the preschool years. The present study examined whether time‐varying changes in parent and child respiratory sinus arrhythmia (RSA) exhibited coregulation (across‐person dynamics) accounting for individual differences in parent and child RSA, and whether there were differences in these parasympathetic processes by children's externalizing problems. Mother‐child dyads (N = 47; Child age M = 3½ years) engaged in three laboratory tasks (free play, clean up, puzzle task) for 18 min, during which RSA data were collected. Multilevel coupled autoregressive models revealed that mothers and preschoolers showed positive coregulation of RSA such that changes in mother RSA predicted changes in the same direction in child RSA and vice versa, controlling for the stability of within‐person RSA over time and individual differences in overall mean RSA. However, when children's externalizing behaviors were higher, coregulation was negative such that changes in real‐time mother and child RSA showed divergence rather than positive concordance. Results suggest that mothers and preschoolers do coregulate RSA during real‐time interactions, but that children's higher externalizing behavior problems are related to disruptions in these processes. © 2015 Wiley Periodicals, Inc. Dev Psychobiol 57: 994–1003, 2015.
Divergence thresholds and divergent biodiversity estimates: can metabarcoding reliably describe zooplankton communities?
Authors: Brown Emily A. , Chain Frédéric J. J. , Crease Teresa J. , MacIsaac Hugh J. , Cristescu Melania E. 
Published: May 2015

AbstractDNA metabarcoding is a promising method for describing communities and estimating biodiversity. This approach uses high‐throughput sequencing of targeted markers to identify species in a complex sample. By convention, sequences are clustered at a predefined sequence divergence threshold (often 3%) into operational taxonomic units (OTUs) that serve as a proxy for species. However, variable levels of interspecific marker variation across taxonomic groups make clustering sequences from a phylogenetically diverse dataset into OTUs at a uniform threshold problematic. In this study, we use mock zooplankton communities to evaluate the accuracy of species richness estimates when following conventional protocols to cluster hypervariable sequences of the V4 region of the small subunit ribosomal RNA gene (18S) into OTUs. By including individually tagged single specimens and “populations” of various species in our communities, we examine the impact of intra‐ and interspecific diversity on OTU clustering. Communities consisting of single individuals per species generated a correspondence of 59–84% between OTU number and species richness at a 3% divergence threshold. However, when multiple individuals per species were included, the correspondence between OTU number and species richness dropped to 31–63%. Our results suggest that intraspecific variation in this marker can often exceed 3%, such that a single species does not always correspond to one OTU. We advocate the need to apply group‐specific divergence thresholds when analyzing complex and taxonomically diverse communities, but also encourage the development of additional filtering steps that allow identification of artifactual rRNA gene sequences or pseudogenes that may generate spurious OTUs.
Engineering Streptomyces diastatochromogenes 1628 to increase the production of toyocamycin
Authors: Tao Libin , Ma Zheng , Xu Xianhao , Bechthold Andreas , Bian Yalin , Shentu Xuping , Yu Xiaoping 
Published: May 2015

Toyocamycin (TM) shows antifungal activity and can be used to treat plant diseases. TM is produced by Streptomyces diastatochromogenes 1628, but the production rate is always very low. Recently, the gene toyG was cloned; however, nothing was known about the influence of toyG on TM production. In this study, the rational combined co‐expression of toyG with frr encoding a ribosome recycling factor and vgb encoding Vitreoscilla hemoglobin was performed. Introduction of these genes in double‐ or triple‐combination into strain S. diastatochromogenes 1628 led to greatly increased TM production. Transcription analysis revealed that higher TM production in the constructed recombinant strains resulted from an increase in transcriptional levels of toyG gene. At the end, among all these different recombinant strains, the strain S. diastatochromogenes 1628‐VGF, harboring vgb, frr, and toyG placed under the control of PermE*, exhibited the largest increase in TM production. Notably, TM produced by S. diastatochromogenes 1628‐VGF reached the highest level of 319.2 mg/L at 72 h, while TM yield of the original strain 1628 was 147.1 mg/L at 84 h. The high‐yield, energy‐saving strain 1628‐VGF constructed in this study shall be beneficial for the industrial production of TM.
Application of pressure‐ and density‐based methods for different flow speeds
Authors: Miettinen A. , Siikonen T. 
Published: May 2015

SummaryIn this paper, pressure‐based and density‐based methods are studied at different flow speeds. The methods are intended for steady flows, and the goal is to find as general an approach as possible to cover different Mach number regimes. The solution methods are based on a finite‐volume approach. Various forms of inviscid fluxes are applied and connected with either a pressure‐based or density‐based implicit solution. For this purpose, a new pressure‐correction method is developed that can be applied for incompressible and for compressible flows. Another option is a standard density‐based approximate factorization method. In both cases, a convergence is accelerated with a Full Approximation Scheme (FAS) multigrid approach. Sample problems in the range of Ma = 0…6 are simulated using different approaches, and their efficiency and accuracy are compared. On the basis of the quality of the solutions, recommendations are made. © 2015 The Authors. International Journal for Numerical Methods in Fluids published by John Wiley & Sons Ltd.
Synthesis of a Fluorescent‐Labeled Bisbenzamidine Containing the Central (6,7‐Dimethoxy‐4‐coumaryl)Alanine Building Block
Authors: Häußler Daniela , Gütschow Michael 
Published: May 2015

ABSTRACTThe synthesis of an amino acid amide is reported, which contains two benzamidine cores, one placed in the amide part the other one within the sulfonyl N‐capping group. The amino acid side chain bears the 6,7‐dimethoxycoumarin fluorophore. The fluorescent amino acid was prepared by using the von‐Pechmann reaction. The two corresponding nitrile groups of a precursor molecule were simultaneously converted to the amidine moieties by the Pinner reaction. The fluorescent properties of the final compound were determined.
Cytotoxicity and intracellular fate of PLGA and chitosan‐coated PLGA nanoparticles in Madin–Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells
Authors: Trif Mihaela , Florian Paula E. , Roseanu Anca , Moisei Magdalena , Craciunescu Oana , Astete Carlos E. , Sabliov Cristina M. 
Published: May 2015

AbstractPolymeric nanoparticles (NPs) are known to facilitate intracellular uptake of drugs to improve their efficacy, with minimum bioreactivity. The goal of this study was to assess cellular uptake and trafficking of PLGA NPs and chitosan (Chi)‐covered PLGA NPs in Madin–Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells. Both PLGA and Chi–PLGA NPs were not cytotoxic to the studied cells at concentrations up to 2500 μg/mL. The positive charge conferred by the chitosan deposition on the PLGA NPs improved NPs uptake by MDBK cells. In this cell line, Chi–PLGA NPs colocalized partially with early endosomes compartment and showed a more consistent perinuclear localization than PLGA NPs. Kinetic uptake of PLGA NPs by Colo 205 was slower than that by MDBK cells, detected only at 24 h, exceeding that of Chi–PLGA NPs. This study offers new insights on NP interaction with target cells supporting the use of NPs as novel nutraceuticals/drug delivery systems in metabolic disorders or cancer therapy. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 3599–3611, 2015.
Massive failure of TiNbN coating in surface engineered metal‐on‐metal hip arthroplasty: Retrieval analysis
Authors: Łapaj Łukasz , Markuszewski Jacek , Wendland Justyna , Mróz Adrian , Wierusz‐Kozłowska Małgorzata 
Published: May 2015

AbstractThis article presents examination of a failed total hip replacement with surface engineered metal‐on‐metal (MoM) articulation. The implant was coated with a thin TiNbN film (Physical Vapor Deposition), and at retrieval 53 months after implantation the coating was abraded on weight‐bearing part of the head and acetabular component. Scanning electron microscopy of bearing surfaces demonstrated multifocal crack formation, delamination of small film fragments, and formation of aggregates containing nanometer sized wear debris. We also observed coating damage in third body mechanism. Complex failure mechanism of TiNbN coating demonstrated in this study suggests insufficient bonding strength between the coating and substrate alloy and raise concerns regarding the use of such coatings in total hip arthroplasty with MoM bearing. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1043–1049, 2016.
The Role of MicroRNA, miR‐24, and Its Target CHI3L1 in Osteomyelitis Caused by Staphylococcus aureus
Authors: Jin Tao , Lu Yong , He Qing Xiao , Wang Hai , Li Bing Fu , Zhu Liang Yue , Xu Qing Yong 
Published: May 2015

ABSTRACTOsteomyelitis is a debilitating infectious disease of the bone which is predominantly caused by Staphylococcus aureus (S. aureus). MicroRNAs (miRNAs) have been shown to play a regulatory role in osteogenesis. In the present study, the expression levels of miRNAs proposed to potentially play a regulatory role in bone formation or differentiation (miR‐24, miR‐29b, miR‐200a, miR‐208, miR‐322) were analyzed in the whole blood of patients with bacterial osteomyelitis or healthy controls, and in MC3T3‐E1 cells infected with S. aureus by qRT‐PCR. The expression of miR‐24 was significantly down‐regulated in osteomyelitis patients and S. aureus‐infected MC3T3‐E1 cells compared with the healthy controls or untreated control cells. Moreover, our results showed that S. aureus inhibited MC3T3‐E1 cell proliferation, induced osteoblast apoptosis and prohibited bone formation and mineralization. We found that overexpression of miR‐24 could reduce the effects of S. aureus, while inhibition of miR‐24 intensified the effects. We also demonstrated that miR‐24 suppressed the expression of chitinase 3‐like 1 (CHI3L1) mRNA, thought to mediate multiple signaling pathways, by directly binding to the 3′‐untranslated region. J. Cell. Biochem. 116: 2804–2813, 2015. © 2015 Wiley Periodicals, Inc.
Metabolite profile of the tomato dwarf cultivar Micro‐Tom and comparative response to saline and nutritional stresses with regard to a commercial cultivar
Authors: Flores Pilar , Hernández Virginia , Hellín Pilar , Fenoll Jose , Cava Juana , Mestre Teresa , Martínez Vicente 
Published: May 2015

AbstractBACKGROUNDThe dwarf tomato variety Micro‐Tom has been used as a plant model for studies of plant development. However, its response to environmental and agricultural factors has not been well studied. This work studies the phytochemical content of Micro‐Tom tomato and its comparative response to saline and nutritional (N, K and Ca) stresses with regard to a commercial variety.RESULTSThe chromatographic profiles of Micro‐Tom were similar to those of the commercial variety and the only differences appear to be the concentration of the components. In Micro‐Tom, the concentrations of sugars and organic acids increased by salinity in a lesser extent than in Optima. Moreover, contrary to that observed in the commercial variety, phenolic compounds and vitamin C did not increase by salinity in the dwarf variety. However, both varieties increased similarly the concentrations of carotenoids under saline conditions. Finally, fruit yield and most primary and secondary metabolite concentrations in Micro‐Tom were not affected by N, K or Ca limitation.CONCLUSIONSThe mutations leading to the dwarf phenotype did not greatly alter the metabolite profiles but studies using Micro‐Tom as a plant model should consider the lower capacity for sugars and organic acids under saline conditions and the greater tolerance to nutrient limitation of the dwarf variety. © 2015 Society of Chemical Industry