bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kaolinite-potassium acetate and halloysite-potasium acetate complexes prepared by mechanochemical, solution and homogenization techniques: a comparative study
Authors: Mako É. , Kovács A. , Horváth E. , Kristóf J. 
Published: July 2014

AbstractKaolinite- and halloysite-potassium acetate complexes were synthesized by cogrinding with solid potassium acetate (mechanochemical intercalation). The efficiency of mechanochemical intercalation was compared to the intercalation in solution and by homogenization. The effects of ageing and grinding parameters (grinding time, sample:grinding body mass ratio (SGMR), rotational speed) and the humidity on the intercalation were studied. The degree of intercalation increased exponentially with ageing of the samples prepared by mechanochemical and homogenization techniques. For the mechanochemical and homogenization techniques the required amount of potassium acetate per gram of kaolin (∼0.4 g/g) was two orders of magnitude lower than that for the solution intercalation (78.6 g/g). The highest degree of intercalation (86%) and the lowest structural deformation were achieved by the mechanochemical method (¼ h of co-grinding with 1:2 SGMR at 300 rpm), followed by 16 h ageing at 57% relative humidity.
Extracellular nucleotide and nucleoside signaling in vascular and blood disease
Authors: Idzko Marco , Ferrari Davide , Riegel Ann-Kathrin , Eltzschig Holger K. 
Published: July 2014

Abstract
Nucleotides and nucleosides—such as adenosine triphosphate (ATP) and adenosine—are famous for their intracellular roles as building blocks for the genetic code or cellular energy currencies. In contrast, their function in the extracellular space is different. Here, they are primarily known as signaling molecules via activation of purinergic receptors, classified as P1 receptors for adenosine or P2 receptors for ATP. Because extracellular ATP is rapidly converted to adenosine by ectonucleotidase, nucleotide-phosphohydrolysis is important for controlling the balance between P2 and P1 signaling. Gene-targeted mice for P1, P2 receptors, or ectonucleotidase exhibit only very mild phenotypic manifestations at baseline. However, they demonstrate alterations in disease susceptibilities when exposed to a variety of vascular or blood diseases. Examples of phenotypic manifestations include vascular barrier dysfunction, graft-vs-host disease, platelet activation, ischemia, and reperfusion injury or sickle cell disease. Many of these studies highlight that purinergic signaling events can be targeted therapeutically.
PKC-β as a therapeutic target in CLL: PKC inhibitor AEB071 demonstrates preclinical activity in CLL
Authors: El-Gamal Dalia , Williams Katie , LaFollette Taylor D. , Cannon Matthew , Blachly James S. , Zhong Yiming , Woyach Jennifer A. , Williams Erich , Awan Farrukh T. , Jones Jeffrey , Andritsos Leslie , Maddocks Kami , Wu Chia-Hsien , Chen Ching-Shih , Lehman Amy , Zhang Xiaoli , Lapalombella Rosa , Byrd John C. 
Published: July 2014

Key PointsAEB071 demonstrates preclinical in vitro and in vivo activity against CLL independent of survival signaling and stromal cell protection. AEB071 can either inhibit or activate the WNT pathway emphasizing the importance of pharmacodynamic monitoring in its development.
Evaluation of mycotoxin sequestering agents for aflatoxin and deoxynivalenol: an in vitro approach
Authors: Kong Changsu , Shin Seung Youp , Kim Beob Gyun 
Published: July 2014

Abstract
An experiment was conducted to determine the efficacy of mycotoxin sequestering agents for binding or degrading aflatoxin B1 (AFB1) and deoxynivalenol (DON) by an in vitro method. Ten toxin binder products including 5 bentonite clays (bentonite A, B, C, D, and E), 2 cellulose products (cellulose A and B), a yeast cell wall, an activated charcoal, and a mixture product containing minerals, microorganisms, and phytogenic substances were used in this experiment. An in vitro procedure was used to mimic the digestive process in pigs. The binding ability for AFB1 of the cellulose products was less compared with the values of other sequestering products (p < 0.05). The percent adsorption of AFB1 by bentonite clays, cellulose products, yeast cell wall product, activated charcoal product, and the mixture product were 92.5 (average of 5 bentonite products), −13.5 (average of 2 cellulose products), 92.7, 100.2, and 96.6, respectively. The respective values for DON were 3.24, 11.6, 22.9, 14.4, and 4.3. In conclusion, most toxin sequestering agents used in the present study had potential to bind AFB1 rather than DON based on the in vitro study which simulated the pH condition of the gastrointestinal tract of pigs.
A comparison of different diagnostic criteria of acute kidney injury in critically ill patients
Authors: Luo Xuying , Jiang Li , Du Bin , Wen Ying , Wang Meiping , Xi Xiuming ,   The Beijing Acute Kidney Injury Trial (BAKIT) workgroup
Published: July 2014

Abstract
Introduction
Recently, the Kidney Disease: Improving Global Outcomes (KDIGO) proposed a new definition and classification of acute kidney injury (AKI) on the basis of the RIFLE (Risk, Injury, Failure, Loss of kidney function, and End-stage renal failure) and AKIN (Acute Kidney Injury Network) criteria, but comparisons of the three criteria in critically ill patients are rare.

Methods
We prospectively analyzed a clinical database of 3,107 adult patients who were consecutively admitted to one of 30 intensive care units of 28 tertiary hospitals in Beijing from 1 March to 31 August 2012. AKI was defined by the RIFLE, AKIN, and KDIGO criteria. Receiver operating curves were used to compare the predictive ability for mortality, and logistic regression analysis was used for the calculation of odds ratios and 95% confidence intervals.

Results
The rates of incidence of AKI using the RIFLE, AKIN, and KDIGO criteria were 46.9%, 38.4%, and 51%, respectively. KDIGO identified more patients than did RIFLE (51% versus 46.9%, P = 0.001) and AKIN (51% versus 38.4%, P <0.001). Compared with patients without AKI, in-hospital mortality was significantly higher for those diagnosed as AKI by using the RIFLE (27.8% versus 7%, P <0.001), AKIN (32.2% versus 7.1%, P <0.001), and KDIGO (27.4% versus 5.6%, P <0.001) criteria, respectively. There was no difference in AKI-related mortality between RIFLE and KDIGO (27.8% versus 27.4%, P = 0.815), but there was significant difference between AKIN and KDIGO (32.2% versus 27.4%, P = 0.006). The areas under the receiver operator characteristic curve for in-hospital mortality were 0.738 (P <0.001) for RIFLE, 0.746 (P <0.001) for AKIN, and 0.757 (P <0.001) for KDIGO. KDIGO was more predictive than RIFLE for in-hospital mortality (P <0.001), but there was no difference between KDIGO and AKIN (P = 0.12).

Conclusions
A higher incidence of AKI was diagnosed according to KDIGO criteria. Patients diagnosed as AKI had a significantly higher in-hospital mortality than non-AKI patients, no matter which criteria were used. Compared with the RIFLE criteria, KDIGO was more predictive for in-hospital mortality, but there was no significant difference between AKIN and KDIGO.
Degradative actions of microbial xylanolytic activities on hemicelluloses from rhizome of Arundo donax
Authors: Lama Licia , Tramice Annabella , Finore Ilaria , Anzelmo Gianluca , Calandrelli Valeria , Pagnotta Eduardo , Tommonaro Giuseppina , Poli Annarita , Di Donato Paola , Nicolaus Barbara , Fagnano Massimo , Mori Mauro , Impagliazzo Adriana , Trincone Antonio 
Published: July 2014

AbstractPolysaccharidases from extremophiles are remarkable for specific action, resistance to different reaction conditions and other biotechnologically interesting features. In this article the action of crude extracts of thermophilic microorganisms (Thermotoga neapolitana,Geobacillus thermantarcticusandThermoanaerobacterium thermostercoris) is studied using as substrate hemicellulose from one of the most interesting biomass crops, the giant reed (Arundo donaxL.). This biomass can be cultivated without competition and a huge amount of rhizomes remains in the soil at the end of cropping cycle (10–15 years) representing a further source of useful molecules. Optimization of the procedure for preparation of the hemicellulose fraction from rhizomes ofArundo donax, is studied. Polysaccharidases from crude extracts of thermophilic microorganisms revealed to be suitable for total degradative action and/or production of small useful oligosaccharides from hemicelluloses fromA. donax. Xylobiose and interesting tetra- and pentasaccharide are obtained by enzymatic action in different conditions. Convenient amount of raw material was processed per mg of crude enzymes. Raw hemicelluloses and pretreated material show antioxidant activity unlike isolated tetra- and pentasaccharide. The body of results suggest that rhizomes represent a useful raw material for the production of valuable industrial products, thus allowing to increase the economic efficiency ofA. donaxcultivation.
Effect of Depression on Diagnosis, Treatment, and Mortality of Men With Clinically Localized Prostate Cancer
Authors: Prasad Sandip M. , Eggener Scott E. , Lipsitz Stuart R. , Irwin Michael R. , Ganz Patricia A. , Hu Jim C. 
Published: July 2014

Purpose Although demographic, clinicopathologic, and socioeconomic differences may affect treatment and outcomes of prostate cancer, the effect of mental health disorders remains unclear. We assessed the effect of previously diagnosed depression on outcomes of men with newly diagnosed prostate cancer. Patients and Methods We performed a population-based observational cohort study using Surveillance, Epidemiology, and End Results-Medicare linked data of 41,275 men diagnosed with clinically localized prostate cancer from 2004 to 2007. We identified 1,894 men with a depressive disorder in the 2 years before the prostate cancer diagnosis and determined its effect on treatment and survival. Results Men with depressive disorder were older, white or Hispanic, unmarried, resided in nonmetropolitan areas and areas of lower median income, and had more comorbidities (P < .05 for all), but there was no variation in clinicopathologic characteristics. In adjusted analyses, men with depressive disorder were more likely to undergo expectant management for low-, intermediate-, and high-risk disease (P ≤ .05, respectively). Conversely, depressed men were less likely to undergo definitive therapy (surgery or radiation) across all risk strata (P < .01, respectively). Depressed men experienced worse overall mortality across risk strata (low: relative risk [RR], 1.86; 95% CI, 1.48 to 2.33; P < .001; intermediate: RR, 1.25; 95% CI, 1.06 to 1.49; P = .01; high: RR, 1.16; 95% CI, 1.03 to 1.32; P = .02). Conclusion Men with intermediate- or high-risk prostate cancer and a recent diagnosis of depression are less likely to undergo definitive treatment and experience worse overall survival. The effect of depression disorders on prostate cancer treatment and survivorship warrants further study, because both conditions are relatively common in men in the United States.
Protection Against High-Fat Diet-Induced Obesity in Helz2-Deficient Male Mice Due to Enhanced Expression of Hepatic Leptin Receptor
Authors: Yoshino Satoshi , Satoh Tetsurou , Yamada Masanobu , Hashimoto Koshi , Tomaru Takuya , Katano-Toki Akiko , Kakizaki Satoru , Okada Shuichi , Shimizu Hiroyuki , Ozawa Atsushi , Tuchiya Takafumi , Ikota Hayato , Nakazato Yoichi , Mori Munemasa , Matozaki Takashi , Sasaki Tsutomu , Kitamura Tadahiro , Mori Masatomo 
Published: July 2014

AbstractObesity arises from impaired energy balance, which is centrally coordinated by leptin through activation of the long form of leptin receptor (Leprb). Obesity causes central leptin resistance. However, whether enhanced peripheral leptin sensitivity could overcome central leptin resistance remains obscure. A peripheral metabolic organ targeted by leptin is the liver, with low Leprb expression. We here show that mice fed a high-fat diet (HFD) and obese patients with hepatosteatosis exhibit increased expression of hepatic helicase with zinc finger 2, a transcriptional coactivator (Helz2), which functions as a transcriptional coregulator of several nuclear receptors, including peroxisome proliferator-activated receptor γ in vitro. To explore the physiological importance of Helz2, we generated Helz2-deficient mice and analyzed their metabolic phenotypes. Helz2-deficient mice showing hyperleptinemia associated with central leptin resistance were protected against HFD-induced obesity and had significantly up-regulated hepatic Leprb expression. Helz2 deficiency and adenovirus-mediated liver-specific exogenous Leprb overexpression in wild-type mice significantly stimulated hepatic AMP-activated protein kinase on HFD, whereas Helz2-deficient db/db mice lacking functional Leprb did not. Fatty acid-β oxidation was increased in Helz2-deficeint hepatocytes, and Helz2-deficient mice revealed increased oxygen consumption and decreased respiratory quotient in calorimetry analyses. The enhanced hepatic AMP-activated protein kinase energy-sensing pathway in Helz2-deficient mice ameliorated hyperlipidemia, hepatosteatosis, and insulin resistance by reducing lipogenic gene expression and stimulating lipid-burning gene expression in the liver. These findings together demonstrate that Helz2 deficiency ameliorates HFD-induced metabolic abnormalities by stimulating endogenous hepatic Leprb expression, despite central leptin resistance. Hepatic HELZ2 might be a novel target molecule for the treatment of obesity with hepatosteatosis.
Germline FH Mutations Presenting With Pheochromocytoma
Authors: Clark Graeme R. , Sciacovelli Marco , Gaude Edoardo , Walsh Diana M. , Kirby Gail , Simpson Michael A. , Trembath Richard C. , Berg Jonathan N. , Woodward Emma R. , Kinning Esther , Morrison Patrick J. , Frezza Christian , Maher Eamonn R. 
Published: July 2014

Abstract

Context:
At least a third of the patients with pheochromocytoma (PCC) or paraganglioma (PGL) harbor an underlying germline mutation in a known PCC/PGL gene. Mutations in genes (SDHB, SDHD, SDHC, and SDHA) encoding a component of the tricarboxylic acid cycle, succinate dehydrogenase (SDH), are a major cause of inherited PCC and PGL. SDHB mutations are also, albeit less frequently, associated with inherited renal cell carcinoma. Inactivation of SDH and another tricarboxylic acid cycle component, fumarate hydratase (FH), have both been associated with abnormalities of cellular metabolism, responsible for the activation of hypoxic gene response pathways and epigenetic alterations (eg, DNA methylation). However, the clinical phenotype of germline mutations in SDHx genes and FH is usually distinct, with FH mutations classically associated with hereditary cutaneous and uterine leiomyomatosis and renal cell carcinoma, although recently an association with PCC/PGL has been reported.

Objective and Design:
To identify potential novel PCC/PGL predisposition genes, we initially undertook exome resequencing studies in a case of childhood PCC, and subsequently FH mutation analysis in a further 71 patients with PCC, PGL, or head and neck PGL.

Results:
After identifying a candidate FH missense mutation in the exome study, we then detected a further candidate missense mutation (p.Glu53Lys) by candidate gene sequencing. In vitro analyses demonstrated that both missense mutations (p.Cys434Tyr and p.Glu53Lys) were catalytically inactive.

Conclusions:
These findings 1) confirm that germline FH mutations may present, albeit rarely with PCC or PGL; and 2) extend the clinical phenotype associated with FH mutations to pediatric PCC.
Uncoupling of Secretion From Growth in Some Hormone Secretory Tissues
Authors: Marx Stephen J. 
Published: July 2014

Context:Most syndromes with benign primary excess of a hormone show positive coupling of hormone secretion to size or proliferation in the affected hormone secretory tissue. Syndromes that lack this coupling seem rare and have not been examined for unifying features among each other.Evidence Acquisition:Selected clinical and basic features were analyzed from original reports and reviews. We examined indices of excess secretion of a hormone and indices of size of secretory tissue within the following three syndromes, each suggestive of uncoupling between these two indices: familial hypocalciuric hypercalcemia, congenital diazoxide-resistant hyperinsulinism, and congenital primary hyperaldosteronism type III (with G151E mutation of the KCNJ5 gene).Evidence Synthesis:Some unifying features among the three syndromes were different from features present among common tumors secreting the same hormone. The unifying and distinguishing features included: 1) expression of hormone excess as early as the first days of life; 2) normal size of tissue that oversecretes a hormone; 3) diffuse histologic expression in the hormonal tissue; 4) resistance to treatment by subtotal ablation of the hormone-secreting tissue; 5) causation by a germline mutation; 6) low potential of the same mutation to cause a tumor by somatic mutation; and 7) expression of the mutated molecule in a pathway between sensing of a serum metabolite and secretion of hormone regulating that metabolite.Conclusion:Some shared clinical and basic features of uncoupling of secretion from size in a hormonal tissue characterize three uncommon states of hormone excess. These features differ importantly from features of common hormonal neoplasm of that tissue.
The Fitzpatrick Skin Type Scale: A reliability and validity study in women undergoing radiation therapy for breast cancer
Authors: Fasugba Oyebola , Gardner Anne , Smyth Wendy 
Published: July 2014

Objective: This study aimed to evaluate the internal consistency reliability and construct validity of the Fitzpatrick Skin Type Scale during radiation therapy in a cohort of women receiving treatment for breast cancer. Method: The assessment of the scale was performed as a nested study within a randomised controlled trial of two creams used for radiation therapy skin care for breast cancer patients. The sample consisted of 244 female patients undergoing radiation therapy for breast cancer. Participants completed a modified version of the Fitzpatrick Skin Type Scale. Results: Internal consistency as measured by Cronbach's alpha was 0.505, 0.829 and 0.339 for the Genetic Disposition, Sun Exposure and Tanning Habits subscales respectively. Only the Sun Exposure subscale surpassed the 0.70 cut-off, indicating good internal consistency. Maximum likelihood factor analysis with promax rotation method confirmed the a priori factor structure for the Sun Exposure subscale as well as providing evidence of construct validity for this subscale. Analysis for the other two subscales highlighted issues with internal reliability and construct validity suggesting that not all items on each subscale truly measure the intended trait. Conclusion: The study findings support reliability and validity of the Sun Exposure subscale of the Fitzpatrick Skin Type Scale in a convenience sample of women receiving radiation therapy for cancer. Despite limitations with two of the three subscales, this tool continues to be used in clinical practice.
Pressure ulcer risk assessment and prevention: What difference does a risk scale make? A comparison between Norway and Ireland
Authors: Johansen E. , Moore Z. , van Etten M. , Strapp H. 
Published: July 2014

Objective: To explore similarities and differences in nurses’ views on risk assessment practices and preventive care activities in a context where patients’ risk of developing pressure ulcers is assessed using clinical judgment (Norway) and a context where patients’ risk of developing pressure ulcers is assessed using a formal structured risk assessment combined with clinical judgement (Ireland). Method: A descriptive, qualitative design was employed across two different care settings with a total of 14 health care workers, nine from Norway and five from Ireland. Results: Regardless of whether risk assessment was undertaken using clinical judgment or formal structured risk assessment, identified risk factors, at risk patients and appropriate preventive initiatives discussed by participant were similar across care settings. Furthermore, risk assessment did not necessarily result in the planning and implementation of appropriate pressure ulcer prevention initiatives. Thus, in this instance, use of a formal risk assessment tool does not seem to make any difference to the planning, initiation and evaluation of pressure ulcer prevention strategies. Conclusion: Regardless of the method of risk assessment, patients at risk of developing pressure ulcers are detected, suggesting that the practice of risk assessment should be re-evaluated. Moreover, appropriate preventive interventions were described. However, the missing link between risk assessment and documented care planning is of concern and barriers to appropriate pressure ulcer documentation should be explored further. Declaration of interest: This work is partly funded by a research grant from the Norwegian Nurses Organisation (NNO) (Norsk Sykepleierforbund NSF) in 2012. The authors have no conflict of interest to declare.
Quantum computation of discrete logarithms in semigroups
Authors: Childs Andrew M. , Ivanyos Gábor 
Published: July 2014

Abstract
We describe an efficient quantum algorithm for computing discrete logarithms in semigroups using Shor's algorithms for period finding and the discrete logarithm problem as subroutines. Thus proposed cryptosystems based on the presumed hardness of discrete logarithms in semigroups are insecure against quantum attacks.
In contrast, we show that some generalizations of the discrete logarithm problem are hard in semigroups despite being easy in groups. We relate a shifted version of the discrete logarithm problem in semigroups to the dihedral hidden subgroup problem, and we show that the constructive membership problem with respect to k ≥ 2 generators in a black-box abelian semigroup of order N requires

Θ
˜

(

N

1
2

-

1

2
k

)

$\tilde{\Theta }(N^{\frac{1}{2}-\frac{1}{2k}})$

quantum queries.
Disruption of Mbd5 in mice causes neuronal functional deficits and neurobehavioral abnormalities consistent with 2q23.1 microdeletion syndrome
Authors: Camarena Vladimir , Cao Lei , Abad Clemer , Abrams Alexander , Toledo Yaima , Araki Kimi , Araki Masatake , Walz Katherina , Young Juan I 
Published: July 2014

Abstract2q23.1 microdeletion syndrome is characterized by intellectual disability, motor delay, autistic‐like behaviors, and a distinctive craniofacial phenotype. All patients carry a partial or total deletion of methyl‐CpG‐binding domain protein 5 (MBD5), suggesting that haploinsufficiency of this gene is responsible for the phenotype. To confirm this hypothesis and to examine the role of MBD5 in vivo, we have generated and characterized an Mbd5 gene‐trap mouse model. Our study indicates that the Mbd5+/GT mouse model recapitulates most of the hallmark phenotypes observed in 2q23.1 deletion carriers including abnormal social behavior, cognitive impairment, and motor and craniofacial abnormalities. In addition, neuronal cultures uncovered a deficiency in neurite outgrowth. These findings support a causal role of MBD5 in 2q23.1 microdeletion syndrome and suggest a role for MBD5 in neuronal processes. The Mbd5+/GT mouse model will advance our understanding of the abnormal brain development underlying the emergence of 2q23.1 deletion‐associated behavioral and cognitive symptoms.