bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE PHENOMENON OF HALO‐LIKE DISAPPEARANCE OF THE MONGOLIAN SPOT ON LESIONS COMBINED WITH CAFÉ AU LAIT SPOT OR ACQUIRED PIGMENTED NEVUS
Authors: Hamada Toshio , Suzuki Shinsuke 
Published: April 2015

ABSTRACTWhen café au lait spots or pigmented nevi were combined with Mongolian spot lesions, whitish haloes were observed in the areas surrounding these café au lait spots and pigmented nevi. The pathogenesis of this halo‐like phenomenon was investigated. The materials were two Japanese girls who had Mongolian spots present at birth over the sacrum and buttocks. In case 1, an eight‐month‐old, café au lait spots and in case 2, a six‐month‐old, brownish black pigmented spots appeared about one month after birth and gradually increased in size and number on the trunk and extremities. In the café au lait spot and pigmented nevus (histologically compound type) lesions and their boundary areas, a whitish halo‐like zone, dermal melanocytes were small and round, as compared with those of surrounding Mongolian spots, although only slightly decreased in number. The characteristic bipolar dendrites were almost imperceptible. The dopa reaction of dermal melanocytes was also decreased. In the boundary halo‐like areas in both cases, epidermal melanin granules and dopa‐positive melanocytes were almost identical to those of the surrounding Mongolian spot.Mongolian spots were already present at birth in both cases, but the café au lait spots and pigmented nevi definitely appeared to arise on these Mongolian spot after birth. The Mongolian spots seemed to disappear at the sites of café au lait spots, pigmented nevi and boundary areas, perhaps a kind of Sutton's phenomenon.
AN AMELANOTIC MELANOMA TRANSFORMED INTO MELANOTIC MELANOMA WITH DEVELOPMENT OF VITILIGO
Authors: Takematsu Hideaki , Tomita Yasushi , Sato Akihiko , Seiji Makoto 
Published: April 2015

ABSTRACTA case of a 77‐year‐old woman with metastatic melanoma and vitiligo‐like leukoderma is reported. The primary lesion had been excised 8 years previously. It had the histologic features of amelanotic melanoma. Metastatic lesions were also amelanotic. Vitiligo‐like leukoderma was noted distant from the cutaneous tumors. The tumors were responsive to a combined regimen of DTIC, ACNU and vincristine and became pigmented after treatment. The relationship between the development of melanized melanoma and chemotherapy is discussed.
CUTANEOUS INVOLVEMENT OF MALIGNANT LYMPHOMA AND MALIGNANT HISTIOCYTOSIS
Authors: Nakanishi Isao , Miwa Atsuo , Katsuda Shogo , Okada Yasunori , Moriizumi Tetsuji , Kaibara Hiroaki , Fujimura Keiko , Tomosugi Naohisa 
Published: April 2015

ABSTRACTMycosis fungoides, lymphosarcoma of a T cell nature with cutaneous involvement, and malignant histiocytosis with primary skin manifestation were presented and their cutaneous lesions were examined with particular emphasis on localization and cytological features of the tumor cells. In addition, six autopsy cases of systemic lymphosarcoma involving the skin and one autopsy case of malignant histiocytosis which showed cutaneous dissemination were reviewed, and a comparative study of the cutaneous lesions of those cases was performed. It was noted that lymphosarcomas tended to infiltrate the lower dermis and underlying subcutaneous tissue, retaining the cell types of tumor cells seen in the primary sites. In malignant histiocytosis, it was suggested that eosinophilia and eosinophilic infiltration in the tissue might indicate longer survival.
INFLUENCE OF VITAMIN E ON THE EFFECT OF VITAMIN A DERIVATIVES ON DIGESTIVE GLANDS OF RATS
Authors: Koh Chang Jo , Lee Jung Bock , Lee Sungnack , Kim Won Joon 
Published: April 2015

ABSTRACTThis study was designed to investigate the influence of d‐α‐tocopheryl acetate on the toxic effect of retinoic acid and retinoid (Ro 10‐9359) on pancreaticobiliary and gastric secretion after the administration of large doses for 3 weeks to albino rats.The toxicity of retinoic acid on body weight and exocrine function, and the morphologic changes in the main digestive glands of the rat are greater than with retinoid.It is clear that vitamin E may suppress or counteract the undesirable toxic effects of retinoic acid and retinoid and appears to promote the function of the main digestive glands of rats.
ADENOSINE DEAMINASE IN HUMAN SKIN
Authors: Kim Young Pio , Kahng Johng Bong , Chon Joon Yul , Ihm Chull Wan 
Published: April 2015

ABSTRACTAdenosine deaminase (adenosine aminohydrolase, ADA), which catalyzes the deamination of adenosine to yield inosine and ammonia, was assayed in human penile foreskin.The skin tissue was separated into two layers; epidermis and dermis. ADA was found to be present in both layers (epidermis, 0.24 ΔoD/mg protein; dermis, 0.19 ΔOD/mg protein) with slightly higher activity in the epidermis. As in earlier reports, it was found that ADA in the skin showed nearly even activity throughout the pH range of 5.0–8.0.Considering the significance of ADA in immunological function, the presence of ADA in the skin suggests that the tissue may participate in the immune function.
LYMPHOCYTE STUDIES IN BULLOUS PEMPHIGOID
Authors: Ahmed A. Razzaque 
Published: April 2015

ABSTRACTLymphocyte studies were performed on 19 untreated patients with active bullous pemphigoid and 35 controls. Peripheral blood T cells, Fc receptor‐bearing cells, B cells and phytohemag‐glutinin (PHA) stimulation were studied. Patients with bullous pemphigoid did not have statistically significant levels of circulating T cells, B cells, or Fc receptor‐bearing cells, compared to normals (P≥0.05). PHA stimulation was significantly lower in bullous pemphigoid patients, P values being 0.006, 0.007 and 0.008 at PHA concentration of 1/2,500, 1/1,000, and 1/100 respectively. This study demonstrates that one of the functions of T cells in the peripheral blood leukocytes from patients with bullous pemphigoid is decreased.
CLINICAL OBSERVATIONS ON A CASE OF IMMUNODEFICIENCY AND THYMOMA (GOOD'S SYNDROME) ASSOCIATED WITH CHRONIC MUCOCUTANEOUS CANDIDIASIS
Authors: Kaneko Fumio , Tsuchiya Kikuo , Miura Yusho , Kishiyama Kazunori , Kusakabe Yasuyuki , Matsumoto Shinobu , Watanabe Makoto 
Published: April 2015

ABSTRACTA 50 year‐old woman with immunodeficiency and thymoma (Good's syndrome) associated with chronic muco‐cutaneous candidiasis (CMCC) was observed. Immunological treatment with transfer factor (TF), supplements ofγ‐globulin and anti‐candidal agents was maintained for six years. Because of the association of pure red cell aplasia in the clinical course, systemic steroid therapy was added to the treatment. Although the patient gradually improved, further immunological therapy was considered to be necessary for the tongue lesions due to candidiasis and the agammaglobulinemia.
DIFFERENTIAL EFFECTS OF 5‐BROMODEOXYURIDINE ON B16 MOUSE MELANOMA CELLS
Authors: Sugita Yuji , Takeuchi Takuji 
Published: April 2015

ABSTRACTThe effects of 5‐bromodeoxyuridine (BrdU) on B16 mouse melanoma were studied and the following results were obtained.

Melanogenesis of cells was completely inhibited by BrdU.
Tyrosinase activity was reduced linearly with time by BrdU, but cytochrome c oxidase was not affected.
The polysome profile from controls and that from BrdU‐treated cells resembled each other when assayed at 260 nm. However, the radioactivity in polysomes in the treated cells was about 30–40% of that in the control, when pulse labeled with radioactive uridine.
Polysomal RNAs were fractionated into poly(A)‐containing and non‐poly(A)‐containing RNA with the use of poly(U)‐Sepharose, and both types of RNA were examined by polyacrylamide gel electrophoresis. No significant differences were detected between the control and the BrdU‐treated cells in either type of RNA.
The effects of BrdU are specific for melanogenesis and the differences in polysomal RNAs might be too small to detect in our experiments. Immunoprecipitation of mRNAs for melanosomal proteins will be necessary to detect persuasive differences.
CLINICOPATHOLOGICAL AND IMMUNOLOGICAL STUDIES OF LUPUS MILIARIS DISSEMINATUS FACIEI
Authors: Shitara Atsuyuki 
Published: April 2015

ABSTRACTEighty‐one cases of lupus miliaris disseminatus faciei were clinically and histologically studied, and some of them were also immunologically studied. Age at onset, distribution of skin lesions, and incidence by sex did not differ from the reports of various investigators. No apparent increase in incidence of this disease was noted. The histologic features of this disease could be divided into four types. Some relation between pilosebaceous units and epithelioid cell granulomas could be found in the lesions of 35 (43%) out of 81 cases. Central caseation may be coagulation necrosis caused by abscesses. Some immunological data were obtained from this study, but there were no abnormalities in delayed hypersensitivity reactions, e.g. tuberculin test, DNCB skin reaction, PHA skin reaction and the Kveim test, or in quantitative serum immunoglobulin surveys. Lymphocyte transformation tests using two mitogens, PHA‐P and Con‐A, showed normal responses. It was not possible to obtain enough data from the clinical data and histologic findings for this disease to be regarded as a tuberculid. But from the histologic findings, lupus miliaris disseminatus faciei was assumed to be a chronic inflammatory disease of pilosebaceous units and epithelioid cell granulomas in the lesions might be reactions to destroyed hair follicles or epithelial cysts. With these assumptions, the following hypotheses concerning the pathogenesis of this disease were proposed. 1) Epithelial cysts occur and rupture in the dermis for some reason resulting in formation of foreign‐body granulomas which break down epithelial cysts in the dermis. 2) Inflammatory infiltrates occur within the follicular epithelium or sebaceous glands for some reason and they are destroyed resulting in formation of epithelioid cell granulomas to follicular contents, such as keratin, sebum andDemodex folliculorum. Under the above assumptions, I would like to support the theory that lupus miliaris disseminatus faciei is not a tuberculid but a skin disorder similar to the papular type of acne rosacea.
EFFECT OF UVA IRRADIATION ON B16 MELANOMA CELLS IN MONOLAYER CULTURE
Authors: Shono Shigeaki , Terauchi Masami , Toda Kiyoshi 
Published: April 2015

ABSTRACTB16 murine melanoma cells in monolayer culture were exposed to a single dose of long wave ultraviolet light (UVA). The number of viable melanoma cells was decreased at 10 hrs post irradiation in parallel to the increase in UVA dose (104~107 ergs/cm2). After ten hours, the cultured cells entered the logarithmic growth phase. The doubling time of the cells exposed to the low dose of UVA was similar to that of nonirradiated cells, but the exposure to higher doses of UVA (105~106 ergs/cm2) elongated the doubling time of these cells. Still higher doses of UVA exposure (107 ergs/cm2) caused no further elongation of doubling time.The tyrosinase activity of these melanoma cells was also measured using an oxygen electrode. The tyrosinase activity of each irradiated cell at ten hours was significantly increased, but no significant increase of tyrosinase activity per mg protein was observed, since protein content per cell increased at the same time. The increase in tyrosinase activity was still observed after ten hours in cells exposed to higher amounts of UVA. These results suggest that UVA repressed cell division and induced protein synthesis to prepare for melanogenesis.
CORRELATIONS BETWEEN COAGULASE TYPES AND ANTIBIOGRAMS OF STAPHYLOCOCCUS AUREUS FROM BACTERIAL SKIN INFECTIONS
Authors: Dekio Satoshi , Onimura Kentaro 
Published: April 2015

ABSTRACTWe investigated coagulase types by a simplified method for coagulase typing and antibiotic sensitivities by a disk diffusion method in 557 strains of Staphylococcus aureus isolated from lesions of various bacterial skin infections. Every strain investigated fell into only one of eight coagulase types by coagulase typing, and obvious correlations were seen between the coagulase types and the anti‐biograms. It was suggested that compatibility‐incompatibility relationships might be present between genes of coagulases of different coagulase types and antibiotic resistance plasmids.
AN ADULT CASE OF KERION CELSI DUE TO TRICHOPHYTON TONSURANS
Authors: Yamasaki Yuuichiro , Toda Michiko , Ikutomi Masaaki , Nishikawa Takeji 
Published: April 2015

ABSTRACTAn adult case of kerion Celsi was reported. The patient was a 28‐year‐old housewife who lived in Tokyo. The fungus isolated was identified as Trichophyton tonsurans, indicating that this rare fungus can be isolated in Tokyo despite the lack of previous reports.
PENICILLIN AND CEPHALOSPORIN—INDUCED RASH IN GUINEA PIGS
Authors: Ikezawa Zenrō , Kitamura Kazuko , Murakami Junko , Nagai Ryukichi , Iwata Masayuki 
Published: April 2015

ABSTRACTGuinea pigs primed for delayed type hypersensitivity (DH) to antibiotics such as penicillin G (PCG), carbenicillin (CBPC), sulbenicillin (SBPC), ampicillin (ABPC), cephalexin (CEX), cephalothin (CET) and cephazolin (CEZ) by immunization in mycobacterial adjuvant developed a generalized rash (GR) and flare‐up of previous test sites when challenged peritoneally with high doses of specific antigen. The GR was delayed in time, and histologically characterized by dilated and compacted small vessels and leucocytic infiltrations into the upper dermis. Animals immunized with CEX or CET developed a strong GR despite the absence of detectable antibodies.Hapten‐carrier specificity and cross‐reactivity in the GR system were essentially the same as those observed in skin testing of DH, but differed from those in the hemagglutination system.Accordingly, the GR shares many of the properties of DH skin test reactions: time course, histology, development despite an absence of detectable antibodies, carrier specificity, cross‐reactivity. This experimentally induced rash may be a useful model for delayed type, generalized, exanthematic drug eruptions in man.
INTRAEPIDERMAL FREE NERVE ENDINGS IN GUINEA PIGS
Authors: Mihara Motoyuki , Hashimoto Ken , Kumakiri Masanobu 
Published: April 2015

ABSTRACTUsing transmission electron microscopy, the intraepidermal free nerve endings in the back skin of spotted black and white guinea pigs were examined. Many intraepidermal free nerve endings were present in both the black areas and the white areas. They were located in the spaces between the basal lamina of the epidermis and the cytomembrane of the basal keratinocyte or in the intercellular spaces between the basal keratinocytes, but never beyond the Malphigian layers. Most of them appeared to belong to sensory nerves and some to adrenergic nerves. They seemed to be related intimately to epidermal keratinocytes.
ASSOCIATION OF PORPHYRIA CUTANEA TARDA AND PRIMARY LIVER CANCER: REPORT OF TEN CASES
Authors: Solis J.A. , Betancor P. , Campos R. , De Salamanca R. Enriquez , Rojo P. , Marin I. , Schüller A. 
Published: April 2015

ABSTRACTStudies of the association between porphyria cutanea tarda (PCT) and primary liver cancer have produced conflicting reports. The incidence of this liver tumor was studied in 138 PCT patients (7.2%) and in 358 non‐porphyric cirrhotic patients (5.6%), and no statistically significant differences were found between them (p>0.5). Nevertheless the incidence of such association was significantly higher (chi square=10.91, p<0.001) in the PCT group (24.3%) than in the non‐porphyric cirrhotic group (6.7%) when only males with underlying liver cirrhosis were considered. These results suggest that the high incidence of liver tumor in PCT is not merely related to hepatic cirrhosis. The cause of such as association remains unclear. Primary liver cancer may reveal a prior deficiency in uroporphyrinogen‐decarboxylase, but the possibility that the enzyme abnormalities were caused by the liver carcinoma also exists.
CHANGES IN ELECTROPHORETIC PATTERNS OF AQUEOUS BUFFER‐INSOLUBLE PROTEINS DURING THE CULTIVATION OF GUINEA PIG EPIDERMAL CELLS
Authors: Toku Seikichi , Aso Kazuo , Hozumi Yutaka , Kondo Shigeo 
Published: April 2015

AbstractThe process of formation of aqueous buffer‐insoluble proteins, which are tentatively regarded as keratins or their precursors, was investigated using epidermal cells cultured from guinea pig ear skin. It was found that cells in the early stages of the culture (proliferation stage) contained a small number of aqueous buffer‐insoluble proteins with molecular weights of 65K, 58K, 57K and 49K. After six days of culture (differentiation stage), cells rapidly formed these proteins, mainly consisting of 58K, 57K, and 49K proteins. In these cultured cells, 70K protein, which has been observed in the whole epidermis (in vivo), was not detected. Addition of the retinoid Ro 10‐1670, an activated form of Ro 10‐9359, into the culture medium accelerated the starting of the formation of these proteins, while inhibiting the formation of 65K and 45K polypeptides.
THE INVOLVEMENT OF THE SEBACEOUS GLAND BY NEVUS CELLS
Authors: Kawamura Taro , Shishiba Takako , Horie Naoshige 
Published: April 2015

AbstractTwo cases of nevocytic nevi with nevus cells in the sebaceous gland were described. In the first case, the nevus seems to have been acquired but in the second case it is apparently congenital. As the sebaceous glands were presumably embryonic when the nevus cells settled into the corium, the immigration of nevus cells into the sebaceous glands might occur at the same time. The possibility of elimination of sebaceous cells by nevus cells is also pointed out.
GENERALIZED PUSTULAR RASH ASSOCIATED WITH PRIMARY ATYPICAL PNEUMONIA
Authors: Matsubara Motoo , Ueda Keiichi , Kishimoto Saburo , Yasuno Hirokazu , Ikada Junji , Sakagami Sachiko , Morioka Yoshihito 
Published: April 2015

AbstractAfter the acute onset of primary atypical pneumonia, a 4‐year‐old girl suddenly developed a widespread, sterile, pustular eruption with predilection toward the distal parts of the extremities. There was no involvement of the muco‐ocular area. Histological and electron microscopical examinations revealed intraepidermal pustule formation containing neutrophils and eosinophils, and perivascular infiltration of lymphocytes, histiocytes, neutrophils and eosinophils. These clinical findings are similar to those of Tan's “acute generalized pustular bacterid” and Teisch's “vesiculopustular eruption with mycoplasma infection”. Serological examinations, including complement fixation test, cold agglutination test, and passive hemagglutination test, confirmed a Mycoplasma pneumoniae infection. It is concluded that Mycoplasma pneumoniae infection should be considered in patients with bacterid‐like rashes who do not have streptococcal infections.
CELL KINETIC STUDIES OF THE EFFECT OF 8‐METHOXYPSORALEN WITH LONG WAVE ULTRAVIOLET IRRADIATION ON HUMAN MELANOMA CELL LINE (SEKI, II)
Authors: Shimada Shinji , Kawashima Makoto , Watanabe Shinichi , Yamada Kiyoshi , Mizoguchi Masako , Hori Yoshiaki , Kukita Atsushi 
Published: April 2015

AbstractThe growth inhibition and cell cycle phase distribution of a human malignant melanoma cell line (SEKI, II) treated with 8‐methoxypsoralen (8MOP) and UVA irradiation (PUVA) were investigated in vitro. A growth inhibition effect was observed in 8 methoxypsoralen (8MOP) 0.1 μg/ml + UVA 1 J/cm2 group, and a strong cytocidal effect was noted in the 8MOP 1 μg/ml + UVA 3 J/cm2 groups. Continued accumulation was observed at 24 to 72 hours in the following phases: G2M phase in the 8MOP 0.1 μg/ml + UVA 1 J/cm2 group; middle S phase in the 8MOP 0.1 μg/ml + UVA 3 J/cm2 group; and early S phase in the 8MOP 1 μg/ml + UVA 1 J/cm2 group. It appeared that 8MOP with UVA irradiation acts mainly on the S phase to block the cell cycle progression between the S and G2M phases. The variation of cell cycle phase distribution under the effect of 8MOP with UVA irradiation was similar to that observed with the alkylating agent, ACNU, suggesting a possible similarity of the mechanism of action between them. The reaction in the dark was vital for the onset of action of 8MOP with UVA irradiation; as is the case for DTIC with UVA irradiation (32).
PLASMA VISCOSITY CHANGES IN CUTANEOUS VASCULITIS
Authors: Nishioka Kiyoshi , Sarashi Chizuko , Sano Shigeharu 
Published: April 2015

AbstractBlood and plasma viscosity in patients with vascular disease was measured using a rotational cone‐plate viscometer. Increased blood viscosity at moderate (76.8 sec–1) and high (384.0 sec–1) shear rates was found in patients with Bürger's disease. Reduced blood viscosity was noted in patients with livedo vasculitis.Plasma viscosity was significantly increased at low (19.2 sec –1), moderate, and high shear rates in patients with leg ulcer, erythema nodosum, and Bürger's disease, in which blood vessels in the lower dermis and subcutaneous tissue were suspected to be affected. Plasma viscosity at low and moderate shear rates was increased in patients with livedo vasculitis and increased plasma viscosity at low shear rate was noted in patients with superficial vasculitis (anaphylactoid purpura and chronic pigmented purpura).Changes in plasma viscosity correlated well with amounts of γ‐globulin, but not with amounts of fibrinogen.
LICHEN PLANUS: EVALUATION OF CELLS IN SKIN LESIONS AND OF T‐LYMPHOCYTE SUBSETS IN BLOOD
Authors: Fernandez‐Bussy Ramon A. , Schmitt Daniel , Gaucherand Martine , Maudutt Gilles , Gomes Manuel A. , Brochier Jean , Thivolet Jean 
Published: April 2015

ABSTRACTThe purpose of the present study was to examine the phenotype of cutaneous immunocompetent cells and to quantify Langerhans cells in Lichen planus by the use of monoclonal antibodies directed against T‐cell populations.Helper cells (OKT4+) and Suppressor/cytotoxic cells (OKT8+) were observed in all cutaneous infiltrates, and numerous Langerhans cells were identified by OKT6, BL6, and BL2 (HLA‐DR) in the epidermis and dermis.The quantification of Langerhans cells demonstrated that the number of dendritic cells in epidermis is greater in involved skin than in non‐involved skin. In recent lesions, Langerhans cells are more abundant than in older lesions.The results in peripheral blood indicated a T Helper/Suppressor imbalance with a decreased T‐Suppressor/cytotoxic subpopulation in patients with lichen planus diseases.In lichen planus, our results suggest an immunological reaction involving all the immunocompetent cell subpopulations with a first stage of information by Langerhans cells (OKT6+, BL6+, BL2+ (HLA‐DR)) and Helper cells (OKT4+), and second stage mediated by Suppressor/cytotoxic cells (OKT8+).
INFLUENCE OF VEHICLES ON PENETRATION THROUGH HUMAN EPIDERMIS OF BENZYL ALCOHOL, ISOEUGENOL AND METHYL ISOEUGENOL
Authors: Jimbo Yuko , Ishihara Masaru , Osamura Hiromi , Takano Masashi , Ohara Mitsuo 
Published: April 2015

ABSTRACTThe influence of 5 liquid, 6 cosmetic and 2 ointment vehicles on the penetration of benzyl alcohol, isoeugenol and methyl isoeugenol through human epidermis was studied. Benzyl alcohol penetrated most rapidly from almost all vehicles. Among liquid vehicles, liquid paraffin provided the best penetration for the 3 compounds. As the ratio of water to ethanol increased in ethanol/water solutions, the penetration of each compound increased. Among cosmetic and ointment vehicles, lotion and milky lotion afforded maximum penetration of each compound with the exception of milky lotion in the case of benzyl alcohol. Macrogol ointment provided the poorest penetration results for the 3 compounds tested.
JUVENILE XANTHOGRANULOMA WITH DARIER'S SIGN
Authors: Nagayo Kinuo , Sakai Miyoko , Mizuno Nobuyuki 
Published: April 2015

ABSTRACTA case of juvenile xanthogranuloma with Darier's sign in a 3‐year‐old boy was described. He had several brown macules on his face, neck, and trunk. When these macules were rubbed with a metal rod, wheal‐like erythema appeared in the area corresponding to them. This phenomenon is called Darier's sign in urticaria pigmentosa. However, the histological picture revealed a xanthogranulomatous reaction, and a few mast cells were seen surrounding the granulomatous area.