bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultraviolet–ozone surface cleaning of injection‐molded, thermoplastic microcantilevers
Authors: Urwyler Prabitha , Pascual Alfons , Müller Bert , Schift Helmut 
Published: January 2015

ABSTRACTUltraviolet–ozone (UVO) is used for the cleaning of labware from organic contamination and includes sterilization, surface roughening, and activation. A range of polymers has been treated without major effects on surface topography and surface chemistry. Even for those, which are subject to physical and chemical aging, a trade‐off between damages and surface cleaning was often found. This communication presents a comprehensive overview on how to UVO‐treat selected polymers known from applications in biology and medicine, in particular microcantilevers used for biosensing. The study provides well‐defined thresholds below which degradation and surface damages are avoided. The impact of UVO treatments on the surface and bulk properties of the injection‐molded polymers was examined by means of differential scanning calorimetry and Fourier transform infrared spectroscopy measurements. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41922.
The Cyber Self: Facebook as a Predictor of Well‐being
Authors: Whitman Chassitty N. , Gottdiener William H. 
Published: January 2015

AbstractGrowing research suggests that Facebook may relate to users’ self‐esteem, identity perceptions, and cognitive function by facilitating identity exploration and minimizing self‐deception. The present study empirically investigated this possibility. A cross‐sectional correlational design with a web‐based survey was utilized. Participants (N = 446) were 24.3% male (N = 118), 67.2% female (N = 326), 2% transgender/in transition (N = 2). 59.6% of participants were ages 18–32 (N = 289) while 13.6% were age 23–27 (N = 66) and 18.3% over age 28 (N = 89). High frequency Facebook use significantly positively correlated with mature defense style (p < 0.05, r = 0.13). High self‐esteem significantly positively correlated with mature defense styles (p < 0.01, r = 0.24) and negatively with immature styles (p < 0.05, r = −0.13). High frequency Facebook use also significantly positively correlated with Actualization Potential (p < 0.01, r = 0.20). In summary, higher levels of Facebook use appear to correlate with positive attributes such as low self‐deception, mature coping, high self‐esteem, and high actualization potential. Copyright © 2015 John Wiley & Sons, Ltd.
DNA topoisomerase‐directed anticancerous alkaloids: ADMET‐based screening, molecular docking, and dynamics simulation
Authors: Singh Swati , Das Tamal , Awasthi Manika , Pandey Veda P. , Pandey Brijesh , Dwivedi Upendra N. 
Published: January 2015

AbstractTopoisomerases (Topo I and II) have been looked as crucial targets against various types of cancers. In the present paper, 100 anticancerous alkaloids were subjected to in silico absorption, distribution, metabolism, excretion, and toxicity (ADMET) analyses to investigate their pharmacokinetic properties. Out of 100 alkaloids, only 18 were found to fulfill all the ADMET descriptors and obeyed the Lipinski's rule of five. All the 18 alkaloids were found to dock successfully within the active site of both Topo I and II. A comparison of the inhibitory potential of 18 screened alkaloids with those of selected drugs revealed that four alkaloids (oliveroline, coptisine, aristolactam, and piperine) inhibited Topo I, whereas six alkaloids (oliveroline, aristolactam, anonaine, piperine, coptisine, and liriodenine) inhibited Topo II more strongly than those of their corresponding drugs, topotecan and etoposide, respectively, with oliveroline being the outstanding. The stability of the complexes of Topo I and II with the best docked alkaloid, oliveroline, was further analyzed using 10 nSec molecular dynamics simulation and compared with those of the respective drugs, namely, topotecan and etoposide, which revealed stabilization of these complexes within 5 nSec of simulation with better stability of Topo II complex than that of Topo I.
Indole generates quiescent and metabolically active Escherichia coli cultures
Authors: Chen Chih‐Chin , Walia Rupali , Mukherjee Krishna J. , Mahalik Subhashree , Summers David K. 
Published: January 2015

AbstractAn inherent problem with bacterial cell factories used to produce recombinant proteins or metabolites is that resources are channeled into unwanted biomass as well as product. Over several years, attempts have been made to increase efficiency by unlinking biomass and product generation. One example was the quiescent cell (Q‐Cell) expression system that generated non‐growing but metabolically active Escherichia coli by over‐expressing a regulatory RNA (Rcd) in a defined genetic background. Although effective at increasing the efficiency with which resources are converted to product, the technical complexity of the Rcd‐based Q‐Cell system limited its use. We describe here an alternative method for generating Q‐Cells by the direct addition of indole, or related indole derivatives, to the culture medium of an E. coli strain carrying defined mutations in the hns gene. This simple and effective approach is shown to be functional in both shake‐flask and fermenter culture. The cells remain metabolically active and analysis of their performance in the fermenter suggests that they may be particularly suitable for the production of cellular metabolites.
Nanoarchitecture of scaffolds and endothelial cells in engineering small diameter vascular grafts
Authors: Sankaran Krishna Kumar , Subramanian Anuradha , Krishnan Uma Maheswari , Sethuraman Swaminathan 
Published: January 2015

AbstractRegeneration of functional small diameter blood vessels still remains a challenge, as the synthetic vascular grafts fail to mimic the complex structural architecture and dynamic functions of blood vessels and also lack with the lack of non‐thrombogenicity. Although, the existence of nanofibrous extracellular matrix components in the native tissue promotes many physical and molecular signals to the endothelial cells for the regulation of morphogenesis, homeostasis, and cellular functions in vascular tissue, poor understanding of the structural architecture on the functional activation of appropriate genes limits the development of successful vascular graft design. Hence, the present review outlines the functional contributions of various nanofibrous extracellular matrix components in native blood vessels. Further, the review focuses on the role of nanofiber topography of biomaterial scaffolds in endothelial cell fate processes such as adhesion, proliferation, migration, and infiltration with the expression of vasculature specific genes; thereby allowing the reader to envisage the communication between the nano‐architecture of scaffolds and endothelial cells in engineering small diameter vascular grafts.
Emotion recognition specialization and context‐dependent risk of anxiety and depression in adolescents
Authors: Oldehinkel Albertine J. , Hartman Catharina A. , Van Oort Floor V. A. , Nederhof Esther 
Published: January 2015

AbstractBackgroundSome adolescents function poorly in apparently benign environments, while others thrive despite hassles and difficulties. The aim of this study was to examine if adolescents with specialized skills in the recognition of either positive or negative emotions have a context‐dependent risk of developing an anxiety or depressive disorder during adolescence, depending on exposure to positive or harsh parenting.MethodsData came from a large prospective Dutch population study (N = 1539). At age 11, perceived parental rejection and emotional warmth were measured by questionnaire, and emotion recognition skills by means of a reaction‐time task. Lifetime diagnoses of anxiety and depressive disorders were assessed at about age 19, using a standardized diagnostic interview.ResultsAdolescents who were specialized in the recognition of positive emotions had a relatively high probability to develop an anxiety disorder when exposed to parental rejection (Bspecialization*rejection = 0.23, P < 0.01) and a relatively low probability in response to parental emotional warmth (Bspecialization*warmth = −0.24, P = 0.01), while the opposite pattern was found for specialists in negative emotions. The effect of parental emotional warmth on depression onset was likewise modified by emotion recognition specialization (B = −0.13, P = 0.03), but the effect of parental rejection was not (B = 0.02, P = 0.72). In general, the relative advantage of specialists in negative emotions was restricted to fairly uncommon negative conditions.ConclusionsOur results suggest that there is no unequivocal relation between parenting behaviors and the probability to develop an anxiety or depressive disorder in adolescence, and that emotion recognition specialization may be a promising way to distinguish between various types of context‐dependent reaction patterns.
A web‐based virtual laboratory for electron probe microanalysis
Authors: Lu Xia , Tian Di , He Yu‐Tong , Yao Li , Fan Run‐Long 
Published: January 2015

ABSTRACTThis paper introduces a novel web‐based virtual laboratory to support teaching electron probe microanalysis (EPMA), specifically operations of the instrument. The virtual lab includes favorable animations to facilitate understanding characteristics of EPMA, the virtual experimental platform for instructive simulations, and quizzes to measure the students’ achievements in learning. To illustrate the instrumental operations, typical experimental processes have been simulated with 3 modules and 34 windows. Students responded to the survey that the virtual lab was very effective in promoting understanding of EPMA and helping them to familiarize with the operations of the instrument and improve the skills before performing the experiment in real lab. © 2015 Wiley Periodicals, Inc. Comput Appl Eng Educ 23:489–498, 2015; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.21620
A commercial, benefit driven and secure framework for elearning in cloud computing
Authors: Sandhu Rajinder , Sood Sandeep K. 
Published: January 2015

ABSTRACTToday's industry demands well‐equipped engineers with practical as well as theoretical knowledge. Providing specialization courses is need of an hour but decline in teacher to student ratio in universities and educational institutions is big hurdle in this vision. Elearning is an ideal solution to this engineering education problem. Traditional elearning platforms are less efficient and prone to sudden failures. In this paper, a cloud‐based commercial and benefit driven elearning system is proposed which has multiple education service providers and separate end user's needs. Proposed system designing and initial assessment is done using Design Science Research (DSR) approach. DSR studies already available knowledge base and formulate requirements of a commercial elearning system. Proposed framework has layered structure consisting of five layers where each layer is dedicated for a specific task. User layer handles all user interactions, management layer manages cloud resources by accurate prediction and cost calculation, security layer provides secure access to data stored on archive layer, archive layer is responsible for proper indexing and storage of data, finally resource layer manages underline hardware of cloud system. Proposed framework is tested against MyGuru2, results indicates that proposed framework is cost effective and provides better QoS requirements. © 2015 Wiley Periodicals, Inc. Comput Appl Eng Educ 23:499–513, 2015; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.21621
Chemical Composition of Pinus sibirica (Pinaceae)
Authors: Rogachev Artem D. , Salakhutdinov Nariman F. 
Published: January 2015

AbstractSiberian pine (Pinus sibirica), also known as Siberian cedar pine and Siberian cedar, is an important plant that has been long used as a source of natural compounds and materials (wood, needles, soft resin, turpentine, colophony). Its chemical composition has been studied well enough; however, to our surprise, no articles that compile the phytochemical data have been published so far. Presumably, this is due to the fact that most of the studies were published in journals difficult to access and not indexed by search systems. This review, for the first time, presents a systematic compilation of available data of secondary metabolites occurring in the needles, shoots, bark, wood, seeds, and oleoresin of Pinus sibirica.
Essential‐Oil Variability of Juniperus deltoides R.P.Adams along the East Adriatic Coast – How Many Chemotypes Are There?
Authors: Rajčević Nemanja , Janaćković Peđa , Dodoš Tanja , Tešević Vele , Marin Petar D. 
Published: January 2015

AbstractThe composition of the essential oils isolated from twigs of ten Juniperus deltoides R.P. Adams populations from the east Adriatic coast was determined by GC‐FID and GC/MS analyses. Altogether, 169 compounds were identified, representing 95.6–98.4% of the total oil composition. The oils were dominated by monoterpenes (average content of 61.6%), which are characteristic oil components of species of the Juniperus section. Two monoterpenes, α‐pinene and limonene, were the dominant constituents, comprising on average 46.78% of the essential oils. Statistical methods were deployed to determine the diversity of the terpene classes and the common terpenes between the investigated populations. These statistical analyses revealed the existence of three chemotypes within all populations, i.e., a α‐pinene, limonene, and limonene/α‐pinene type.
Profiling and Elucidation of the Phenolic Compounds in the Aerial Parts of Gynura bicolor and G. divaricata Collected from Different Chinese Origins
Authors: Chen Jian , Mangelinckx Sven , Lü Han , Wang Zheng‐Tao , Li Wei‐Lin , De Kimpe Norbert 
Published: January 2015

AbstractGynura bicolor and G. divaricata are not only known to be nutritive as cultured vegetables, but also beneficial as folk medicines in East Asia. As demonstrated by the current phytochemical knowledge, the genus Gynura is a promising source of phenolics with multiple medicinal activities. To expand this phytochemical knowledge, the phenolic secondary metabolites of G. bicolor and G. divaricata were studied. From the aerial parts of these two species, collected in five different Chinese locations, two fractions of phenolic compounds with different polarity were obtained by extraction and chromatographic separation. Using UPLC/MS/MS analysis, a total of 53 phenolics were either identified by comparison with respective reference compounds or tentatively characterized by their chromatographic behavior, UV‐absorption patterns, and MS fragmentations. Some naturally existing positional isomers of O‐caffeoylquinic acid, O‐p‐coumaroylquinic acid, O‐feruloylquinic acid, and dicaffeoylquinic acid as well as their methyl esters were qualitatively characterized by their specific fragmentation patterns in targeted MS/MS. In addition, the aerial parts of the two Gynura species contained kaempferol, quercetin oligoglycosides, and a variety of derivatives of benzoic acid, hydroxycinnamic acid, and caffeic acid. Furthermore, the distribution of phenolic compounds in the two species from different Chinese origins was discussed. Finally, an investigation of the total phenolic content and in vitro antioxidant activity of the various phenolic fractions was completed, to evaluate the potential of the extracts of these species for medicinal development. The free‐radical‐scavenging activities of the extracts derived from plants originating from Nanjing were proven to be higher than those of the other extracts, which correlated well with their total phenolic content.
Discovery and Pharmacophore Studies of Novel Pyrazole‐Based Anti‐Melanoma Agents
Authors: Li Qing‐Shan , Lü Xian‐Hai , Yang Yang , Ruan Ban‐Feng , Yao Ri‐Sheng , Liao Chen‐Zhong 
Published: January 2015

AbstractDue to the rising incidence and lack of effective treatments, malignant melanoma is the most dangerous form of skin cancer, so that new treatment strategies are urgently needed. Several recent developments indicate that the V600E mutant BRAF (BRAFV600E) is a validated target for antimelanoma‐drug development. Based on in silico screening results, a series of novel pyrazole derivatives has been designed, synthesized, and evaluated in vitro for their inhibitory activities against BRAFV600E melanoma cells. Compound 3d exhibited the most potent inhibitory activity with an IC50 value of 0.63 μM for BRAFV600E and a GI50 value of 0.61 μM for mutant BRAF‐dependent cells. Furthermore, the QSAR modeling and the docking simulation of inhibitor analogs provide important pharmacophore clues for further structural optimization.
Two New Hydronaphthoquinones from Sinningia aggregata (Gesneriaceae) and Cytotoxic Activity of Aggregatin D
Authors: Verdan Maria Helena , Mera de Souza Lauro , Ernesto de Carvalho João , Vendramini Costa Débora B. , Salvador Marcos José , Barison Andersson , Alves Stefanello Maria Élida 
Published: January 2015

AbstractTwo new hydronaphthoquinones, aggregatins E and F (1 and 2, resp.) were isolated from the tubers of Sinningia aggregata (Ker‐Gawl.) Wiehler (Gesneriaceae), along with twelve known compounds aggregatin D (3), tectoquinone (4), 1‐hydroxy‐2‐methylanthraquinone (5), icosyl ferulate (6), pustuline (7), 1,6‐dihydroxy‐2‐methylanthranquinone (8), 6‐hydroxy‐2‐methylanthraquinone (9), 7‐hydroxy‐2‐methylanthraquinone (10), tyrosol (11), halleridone (12), calceolarioside B (13), and cornoside (14). All compounds were identified by analysis of spectroscopic and spectrometric data. Compounds 3, 4, and 10 had already been reported in this species. Compounds 2 and 3 were evaluated against several tumor cell lines, but only 3 exhibited activities against UACC‐62, 786‐0 and OVCAR‐3 cell lines, with IC50 values of 12.3, 12.8 and 0.3 μg/ml, respectively, without toxic effects on non‐cancer cell line HaCat (human keratinocyte).
Severe mitral regurgitation: A common occurrence in the aging patient with secundum atrial septal defect
Authors: Liberthson R. R. , Boucher C. A. , Fallon J. T. , Buckley M. J. 
Published: January 2015

AbstractSevere mitral valve regurgitation (MR) which necessitated mitral valve replacement was identified in 19 (3.9%) of 498 consecutive patients (age range 1–83 years) with secundum atrial septal defect (ASD). The incidence of severe MR was significantly higher in patients older than age 50 years, 15 of 98 (15%), than in patients either below 21 years, 1 of 213 (0.4%), or between ages 21 to 49 years, 3 of 187 (2%). The higher frequency and severity of MR in the older ASD patient has not previously been appreciated.The morphology of severe MR in the older ASD patient consists of fibrous thickening and deformity of the mitral leaflets with shortening and thickening of the chordae tendineae. Because of the rarity of severe MR in the young patient with ASD, the mitral valve pathology is still poorly defined.
Neuroleptanalgesia in acute myocardial infarction: Effects of hemodynamic parameters and plasma catecholamines
Authors: Magometschnigg D. , Hörtnagl H. , Gassner A. , Hammerle A. F. , Kleinberger G. , Lenzhofer R. , Lochs H. , Pall H. , Pichler M. 
Published: January 2015

AbstractThe use of neuroleptanalgesia in acute myocardial infarction offers the possibility of reducing pain and emotional stress. The influence of such treatment on hemodynamic parameters (heart rate, cardiac output, stroke volume, peripheral resistance, systemic blood pressure, and pulmonary pressure) and on the plasma level of adrenaline and noradrenaline has been studied in 6 patients with acute myocardial infarction. The results demonstrate that during neuroleptanalgesia the already elevated levels of noradrenaline and adrenaline further increase. This increase was most pronounced in the patients with the highest initial levels of catecholamines. Since the peripheral resistance and systolic and diastolic blood pressures decrease concomitantly, it is concluded that the increase in plasma catecholamines is due to a reaction of the sympathetic nervous system to the alpha‐adrenergic receptor blocking activity of droperidol causing vasodilation. The data indicate that pain, emotional stress, and anxiety in the acute phase of myocardial infarction do not play the expected essential role for the activation of the sympathetic nervous system generally observed in acute myocardial infarction. Additionally, the data demonstrate that drugs producing a vasodilation can have a deteriorating effect on the hemodynamic situation and that a reduction of the afterload by vasodilating drugs can result in a further increase in the release of catecholamines.
Effects of molsidomine on global and regional left ventricular function at rest and during exercise in patients with angina pectoris
Authors: Nechwatal W. , Stauch M. , Sigel H. , Kress P. , Bitter F. , Geffers H. , Adam W. E. 
Published: January 2015

AbstractAlthough the antianginal properties of molsidomine are well‐established, little is known about its effects on global and regional left ventricular dysfunction secondary to myocardial ischemia. In the present study, left ventricular performance was assessed by radionuclide ventriculography at rest and during exercise in 15 patients with coronary artery disease (CAD) and angina pectoris before and after the administration of 2 mg molsidomine sublingually. Gated blood pool studies were performed for evaluation of left ventricular ejection fraction (LVEF) and regional wall motion by analyzing amplitudes and phases of the first Fourier coefficient of regional time–activity curves.In contrast to normal subjects, during the control study period LVEF in patients with CAD decreased from 50.9% at rest to 42.7% during exercise (p<0.01). After molsidomine the resting values of LVEF increased slightly from 50.9% to 55.7% (p<0.05). Exercise values of LVEF increased from 42.7% to 51.3% (p<0.01). This is usually associated with amelioration of anginal pain and ischemic ST depression in the precordial ECG (0.15 mV vs. 0.09 mV; p<0.01). Before molsidomine, regional wall motion deteriorated from rest to exercise in 11 of 15 patients. These wall motion abnormalities usually expressed themselves as newly developed regions of left ventricular dysfunction (8 patients) or as accentuation of pre‐existing contraction disturbances (3 patients). After molsidomine, regional wall motion did not show consistent changes at rest. Comparison during exercise showed enhanced regional function in 10 of the 15 patients after administration of the drug. At rest a slight but significant increase in heart rate was measured following molsidomine, whereas exercise heart rate remained unchanged. Only minor changes in systolic blood pressure occurred after molsidomine (rest, 143 mmHg vs. 134 mmHg; p<0.05; exercise, 177 mmHg vs. 174 mmHg; p>0.10).In conclusion, assessment of left ventricular performance at rest and during exercise in patients with CAD revealed significant improvement of global and regional left ventricular function, indicating reduction of myocardial ischemia. These effects may result primarily from reduction of left ventricular wall tension.