bluuebunny/crossref_metadata_2025_split huggingface dataset:
Information, certainty, and learning
Authors: Harris Justin A. , Gallistel C. R. 
Published: August 2024

ABSTRACTMore than four decades ago, Gibbon and Balsam (1981) showed that the acquisition of Pavlovian conditioning in pigeons is directly related to the informativeness of the conditioning stimulus (CS) about the unconditioned stimulus (US), where informativeness is defined as the ratio of the US-US interval (C) to the CS-US interval (T). However, the evidence for this relationship in other species has been equivocal. Here, we describe an experiment that measured the acquisition of appetitive Pavlovian conditioning in 14 groups of rats trained with differentC/Tratios (ranging from 1.5 to 300) to establish how learning is related to informativeness. We show that the number of trials required for rats to start responding to the CS is determined by theC/Tratio and, remarkably, the specific scalar relationship between the rate of learning and informativeness aligns very closely to that previously obtained with pigeons. We also found that the response rate after extended conditioning is strongly related toT, with the terminal CS response rate being a scalar function of the CS reinforcement rate (1/T). Moreover, this same scalar relationship extended to the rats’ response rates during the (never-reinforced) inter-trial interval, which was directly proportional to the contextual rate of reinforcement (1/C). The findings establish that animals encode rates of reinforcement, and that conditioning is directly related to how much information the CS provides about the US. The consistency of the data across species, captured by a simple regression function, suggests a universal model of conditioning.
Experience of contraceptive care by midwives for nonpostpartum individuals in the Netherlands: a mixed methods study
Authors: Sprenger Merel , Newton Megan D. , Finkenflügel Renee N.N. , Crone Matty R. , Kiefte-de Jong Jessica C. , Slagboom M. Nienke 
Published: August 2024

AbstractSince 2015, Dutch midwives have been authorised to prescribe all contraception. Initially providing contraceptive care to postpartum clients, they are increasingly offering it to anyone. It remains unknown how this broader population experiences this care. Therefore, this mixed methods study aims to explore experiences of nonpostpartum individuals receiving contraceptive care from Dutch primary care midwives.At 13 midwifery practices in the Netherlands, participants were recruited to fill out a survey and participate in an in-depth semi-structured interview, both based on Levesque’s Conceptual Framework of Access to Health. Univariate and multivariate logistic regression analyses were applied to survey data (n = 91) and thematic analysis to interview data (n = 10).Most survey participants (87.8%) received an intrauterine device during their appointment. A majority (58.2%) rated their care a 10 out of 10. Giving full marks was significantly associated with a higher perceived income (adjusted OR = 3.19, 95% CI = 1.21-8.81, p = 0.021), adjusted for appointment type and time since appointment. Participants reported receiving understandable information, being taken seriously, and having enough time during their appointment. Interviews revealed that participants especially appreciate how midwives make them feel at ease, midwives’ expertise, and the convenience of access.To conclude, given the positive experiences reported by nonpostpartum individuals with contraceptive care from midwives, efforts should be made to improve task sharing and to increase awareness of midwives as contraception providers. Future research should compare care experiences across all types of providers and include a more representative population.Statement of significance
Endocytosis of the plasma membrane carboxylate transporter Jen1 inSaccharomyces cerevisiaeunder alkali stress: a mechanism for preventing loss of essential metabolites
Authors: Barata-Antunes Cláudia , de Hulster Erik , Cardoso Clara , Ribeiro Inês P. , Fernandes Vítor , Leite Beatriz , Casal Margarida , Gerós Hernâni , Pronk Jack , Ariño Joaquín , Mans Robert , Paiva Sandra 
Published: August 2024

AbstractWhen grown on lactate as a sole carbon source,Saccharomyces cerevisiaecells alkalinize their surroundings. This alkalinization coincides with endocytosis of the Jen1 lactate transporter. Previous studies showed that this process depends on a functional Jen1 transporter and involves Bul1 α-arrestin and an active TORC1 complex. However, it remained unclear whether the internalization of Jen1 triggered by prolonged growth on lactate was exclusively due to the increase in external pH. To further investigate the regulatory mechanisms underlying the alkali stress-induced Jen1 endocytosis,S. cerevisiaecells stably expressing aJEN1-GFPfusion were studied in aerobic carbon-limited continuous cultures subjected to a linear increase of the culture pH. We found that, in these controlled bioreactor cultures, Jen1 internalization is strongly dependent on extracellular pH, showing a sharp transition at pH values of 7.0 to 7.2. This pH threshold coincides with wash-out of the chemostat cultures and with release of pyruvate and acetate. Transcriptome analysis revealed upregulation of genes involved in oxidative phosphorylation around this threshold, which is consistent with higher cellular energy requirements or energy metabolism being compromised. The originality of this study lies in the use of continuous cultures to analyse the endocytosis of a yeast nutrient transporter. This approach allowed the rigorous study of cell responses to a specific alteration in a single environmental condition, such as changes in pH.
Valorisation of spent cultivated meat media for recombinant FGF2 production in GRASLactococcus lactis
Authors: Rizal Juliana , Mainali Prashant , Quek Jun Ping , Lee Ling Tan , Bi Jiawu , Chan Alson Jianchen , Gaffoor Azra Anwar , Ming Chew Lamony Jian , Sugii Shigeki , Ng Say Kong , Ow Dave Siak-Wei , Wong Fong Tian 
Published: August 2024

AbstractInnovative strategies for sustainable utilization of waste resources are imperative in the pursuit of a circular economy. Recently, the idea of utilizing mammalian spent media as a valuable resource is gaining traction, offering significant opportunities for innovative uses as a food-grade feedstock for microbial fermentation, especially in the production of alternative proteins for research and food purposes.In this study, we aim to repurpose spent mammalian culture media for production of valuable proteins. Growth factors (GFs) are a family of high-value proteins that naturally stimulate cell proliferation or differentiation. More importantly, these factors also present significant costs for cell culture. Here, we successfully demonstrate the use of spent mammalian culture media for the recombinant production of fibroblast growth factor 2 (FGF2-G3) inLactococcus lactis. Bioreactor fermentation at a 1 L scale confirmed purified yields of 2.6 mg/L of recombinant FGF2-G3 using spent media. Further functional testing indicated that the recombinant FGF2-G3 can promote cell proliferation on anAnguilla japonica(Japanese eel) pre-adipocytic cell line, suggesting its potential for cultivated meat production. Based on the preliminary results of this study, our calculations indicate that fermenting 1 L spent mammalian waste could yield enough growth factors to efficiently grow approximately 52 L of cultivated meat through fermentation. This prediction emphasizes the potential of waste valorisation to sustainably produce protein, thus contributing to environmental preservation and economic viability.HighlightsSpent mammalian culture media can be repurposed for microbial fermentation.GRASL. lactiswas used to express FGF2-G3 in an optimized spent media formulation.Functional FGF2-G3 expression was demonstrated in controlled 1-L bioreactor runs.
Characterizing the dual regulation ofNeisseria gonorrhoeae tdfJin response to zinc and iron
Authors: Padmanabhan Sandhya. , Stoudenmire Julie Lynn , Branch Alexis Hope , Cornelissen Cynthia Nau 
Published: August 2024

ABSTRACTGonorrhea is a sexually transmitted infection, caused by the bacterial pathogenNeisseria gonorrhea(Ngo) and affects millions of individuals of all age groups across the globe every year. Infection withNgodoes not result in protection and no effective vaccine has been developed, leaving antibiotics as the only treatment option. With the emergence of strains showing high levels of antibiotic resistance, there is an urgent need for development of novel therapeutics for disease prevention. During pathogenesis the host employs nutritional immunity, to restrict important transition metals such as zinc away fromNgo. This process is counteracted inNgoby the production of highly efficient zinc import TonB-dependent Transporters (TDTs) which are promising vaccine antigens and zinc shuttle ABC transporters found to be important for intracellular survival. InNgozinc homeostasis and transport proteins are regulated by the Zinc uptake regulator (Zur) which represses transcription in the presence of zinc and activates transcription in the absence of zinc. In this study, characterize the promoter elements of the zinc import TDT,tdfJ, which results in dual regulation by zinc and iron. We characterize specificity and binding affinities for regulation oftdfJby a second regulator, Ferric uptake regulator (Fur) in response to iron. The response oftdfJto both iron and zinc and its potential to be an important invasin, makes it an attractive candidate to investigate female genital tract infections. The female genital tract is a conglomerate of these conditions and infections here are often asymptomatic. Taken together, this research provides important knowledge on the regulation of virulence mechanisms in response to zinc, which will aid in the development of therapeutics and an efficacious vaccine against a gonococcal infection.
A holistic lens on entrepreneurial learning from failure: continuing the legacy of Jason Cope
Authors: Lattacher Wolfgang , Wdowiak Malgorzata Anna , Schwarz Erich J. , Audretsch David B. 
Published: August 2024

PurposeThe paper follows Jason Cope's (2011) vision of a holistic perspective on the failure-based learning process. By analyzing the research since Cope's first attempt, which is often fragmentary in nature, and providing novel empirical insights, the paper aims to draw a new comprehensive picture of all five phases of entrepreneurial learning and their interplay.Design/methodology/approachThe study features an interpretative phenomenological analysis of in-depth interviews with 18 failed entrepreneurs. Findings are presented and discussed in line with experiential learning theory and Cope's conceptual framework of five interrelated learning timeframes spanning from the descent into failure until re-emergence.FindingsThe study reveals different patterns of how entrepreneurs experience failure, ranging from abrupt to gradual descent paths, different management and coping behaviors, and varying learning effects depending on the new professional setting (entrepreneurial vs non-entrepreneurial). Analyzing the entrepreneurs' experiences throughout the process shows different paths and connections between individual phases. Findings indicate that the learning timeframes may overlap, appear in different orders, loop, or (partly) stay absent, indicating that the individual learning process is even more dynamic and heterogeneous than hitherto known.Originality/valueThe paper contributes to the field of entrepreneurial learning from failure, advancing Cope's seminal work on the learning process and -contents by providing novel empirical insights and discussing them in the light of recent scientific findings. Since entrepreneurial learning from failure is a complex and dynamic process, using a holistic lens in the analysis contributes to a better understanding of this phenomenon as an integrated whole.
Leveraging the interplay of Internet of Things (IoT) capabilities for achieving customer satisfaction in a B2B2C context: an integrative perspective
Authors: Chand Pushpendu , Tarei Pradeep Kumar 
Published: August 2024

Purpose
Despite IoT’s huge potential, enterprises’ ability to leverage it is their competitive advantage. Thus, competitive differentiation is primarily predicated on leveraging IoT toward customer needs. To examine the research gap, this study aims to explore the drivers of customer satisfaction and how they are affected by the interaction between IoT capabilities.

Design/methodology/approach
A mixed-method research framework is applied to assess the impact of IoT capabilities on customer satisfaction. Based on the theoretical underpinning of the resource-based view and dynamic capability, the study highlights the importance of IoT capabilities in active resource allocation and effective resource utilization. First, DEMATEL is used capture the interrelationship between IoT capabilities. Further, the impact of each IoT capabilities on customer satisfaction is studied using CoCoSo method.

Findings
The study highlights the importance of IoT capabilities in active resource allocation and effective resource utilization. The findings are enriched through the complementarity of resources in a dynamic business-to-business-to-customer (B2B2C) scenario. The authors expand the IoT capabilities from conventional business-to-business (B2B) or business-to-customer (B2C) scenario to tri-nodal B2B2C relationship triangle.

Practical implications
Based on the findings, the authors offer a business transformation strategy for firms in key areas of customer satisfaction by leveraging IoT. The study can help management prioritize and develop key IoT capabilities to meaningfully increase customer satisfaction metrics.

Originality/value
Building on the dynamic capabilities and resource-based view of the firm, an integrated decision-making research model is proposed. In addition, this study investigates the product and service capabilities unlocked using IoT capabilities. This work can be considered one of the leading attempts to improve customer satisfaction using IoT capabilities from traditional dyadic (B2B or B2C) structure to triadic (B2B2C) framework.
The impact of workforce environment on CSR audit report lag: the moderating role of media coverage
Authors: Moalla Marwa , Bouaziz Dhouha , Jarboui Anis 
Published: August 2024

Purpose
The purpose of this study is to investigate the relationship between the workforce environment and corporate social responsibility (CSR) audit report lag while also developing a comprehensive understanding of the moderating effect of media coverage on this relationship.

Design/methodology/approach
This paper was based on a sample of 151 French nonfinancial companies listed on the CAC All Shares index and covered an eight-year period, from 2014 to 2021. To test the hypotheses, a feasible generalized least squares regression was applied. Moreover, the authors checked the results using an additional analysis and the generalized method of moment model for endogeneity problems.

Findings
Based on a panel data set comprising 960 observations of French firms from the period 2014 to 2021, the results obtained indicate a significant negative relationship between the workforce environment and CSR audit report lag. Additionally, it was found that media exposure moderates the relationship between the workforce environment and CSR audit report lag.

Practical implications
This study contributes to the existing research on workforce environment and CSR audit report lag, potentially providing stakeholders such as employees, employers, regulators and auditors with an environment that shortens the time for issuing CSR audit reports. The findings are also relevant for foreign institutional investors aiming to enhance their investment decisions with more comprehensive information.

Originality/value
The work is innovative as it explores the moderating impact of media exposure on the connection between workforce environment and CSR audit report lag, a topic not extensively studied before. To the best of the authors’ knowledge, no prior empirical studies have examined this relationship within the French context or elsewhere.
Pride of the East: Motorcycle Speedway, Transnational Encounters and Provincial Heartlands
Authors: MILLS RICHARD 
Published: August 2024

AbstractFor a century, speedway has operated on a transnational basis. Its riders, competitions and machinery regularly traverse national and continental borders. The imposition of the Cold War's Iron Curtain did little to impede its growth. This article focuses on one of speedway's far‐flung and diverse provincial heartlands to show how the sport generated frequent transnational and transsystemic interactions and forged deep relationships that often stood in contrast to the prevailing trends of international politics. As one of speedway's ‘entangled peripheries’, rural East Anglia owes a debt of gratitude to Australasian pioneers. From the 1950s—decades before imported talent became commonplace in football—the region welcomed Scandinavian and Eastern Bloc riders and hosted visiting clubs and national representations from across the continent. Later, East Anglia became a bridgehead for the successful assault of communist‐built machinery on the Western market. These bold endeavours were not without controversy, as British riders voiced objections to foreign men and machines deemed a threat to their livelihoods. The Cold War's end accelerated existing trends and also created exciting new opportunities for itinerant East Anglians abroad. Archival material, conversations with the sport's foreign trailblazers and fans, Swedish and Czechoslovak sources, photographs and official publications serve to demonstrate speedway's enduring ability to forge unexpected ties and give voice to regions at the so‐called margins of twentieth‐century history.
Consequences of the deletion of the major specialized metabolite biosynthetic pathways of Streptomyces coelicolor on the metabolome and lipidome of this strain
Authors: Lejeune Clara , Abreu Sonia , Guérard Florence , Askora Ahmed , David Michelle , Chaminade Pierre , Gakière Bertrand , Virolle Marie‐Joelle 
Published: August 2024

AbstractChassis strains, derived from Streptomyces coelicolor M145, deleted for one or more of its four main specialized metabolites biosynthetic pathways (CPK, CDA, RED and ACT), in various combinations, were constructed for the heterologous expression of specialized metabolites biosynthetic pathways of various types and origins. To determine consequences of these deletions on the metabolism of the deleted strains comparative lipidomic and metabolomic analyses of these strains and of the original strain were carried out. These studies unexpectedly revealed that the deletion of the peptidic clusters, RED and/or CDA, in a strain deleted for the ACT cluster, resulted into a great increase in the triacylglycerol (TAG) content, whereas the deletion of polyketide clusters, ACT and CPK had no impact on TAG content. Low or high TAG content of the deleted strains was correlated with abundance or paucity in amino acids, respectively, reflecting high or low activity of oxidative metabolism. Hypotheses based on what is known on the bio‐activity and the nature of the precursors of these specialized metabolites are proposed to explain the unexpected consequences of the deletion of these pathways on the metabolism of the bacteria and on the efficiency of the deleted strains as chassis strains.
Modulation of circulating free testosterone fraction by testosterone, dihydrotestosterone, and estradiol during testosterone replacement therapy
Authors: Jasuja Ravi , Pencina Karol M. , Lawney Brian , Stephens‐Shields Alisa J. , Ellenberg Susan S. , Snyder Peter J. , Bhasin Shalender 
Published: August 2024

AbstractBackgroundTestosterone, estradiol, and dihydrotestosterone share common ligand binding sites on sex hormone binding globulin and albumin. It is unknown whether and how changes in testosterone, dihydrotestosterone, and estradiol concentrations during testosterone replacement therapy affect free testosterone fraction.ObjectiveTo determine the effect of changes in testosterone, dihydrotestosterone, and estradiol concentrations on free testosterone fraction during testosterone replacement therapy of men with hypogonadism.MethodsUsing data from the Testosterone Trials, we assessed the association of changes in total testosterone, estradiol, and dihydrotestosterone concentrations over 12 months of testosterone replacement therapy with changes in free testosterone fraction, measured using equilibrium dialysis. We used random forests to evaluate the associations of predicted mean changes in free testosterone fraction with changes in circulating concentrations of each hormone at low, mean, or high change in the other two hormones.ResultsTestosterone replacement therapy not only increased total testosterone, dihydrotestosterone, estradiol, and free testosterone concentrations, but also the percent free testosterone, even though sex hormone binding globulin levels did not change. The predicted changes in free testosterone fraction during testosterone replacement therapy exhibited a non‐linear relationship with changes in each of total testosterone, dihydrotestosterone, and estradiol concentrations. Greater increases in testosterone, dihydrotestosterone, and estradiol levels during testosterone replacement therapy were each associated with higher model‐predicted percent free testosterone. Substantially smaller changes in molar concentrations of estradiol and dihydrotestosterone had a greater effect on percent free testosterone than those in testosterone.ConclusionDuring testosterone replacement therapy of men with hypogonadism, changes in testosterone, dihydrotestosterone, and estradiol concentrations each altered percent free testosterone non‐linearly. Small changes in estradiol concentrations exerted much larger effect on the free testosterone fraction than testosterone and dihydrotestosterone, suggesting complex interactions of the three hormones with the binding proteins. Assessment of changes in free testosterone during testosterone replacement therapy should include consideration of changes in all three hormones.
Turbinal bones are still one of the last frontiers of the tetrapod skull: hypotheses, challenges and perspectives
Authors: Martinez Quentin , Amson Eli , Ruf Irina , Smith Timothy D. , Pirot Nelly , Broyon Morgane , Lebrun Renaud , Captier Guillaume , Gascó Martín Cristina , Ferreira Gabriel , Fabre Pierre‐Henri 
Published: August 2024

ABSTRACTTurbinals are bony or cartilaginous structures that are present in the nasal cavity of most tetrapods. They are involved in key functions such as olfaction, heat, and moisture conservation, as well as protection of the respiratory tract. Despite recent studies that challenged long‐standing hypotheses about their physiological and genomic correlation, turbinals remain largely unexplored, particularly for non‐mammalian species. Herein, we review and synthesise the current knowledge of turbinals using an integrative approach that includes comparative anatomy, physiology, histology and genomics. In addition, we provide synonyms and correspondences of tetrapod turbinals from about 80 publications. This work represents a first step towards drawing hypotheses of homology for the whole clade, and provides a strong basis to develop new research avenues.