bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of the outdoor performance of a dye solar cell mini‐panel for building integrated photovoltaics applications
Authors: Cornaro Cristina , Bartocci Simona , Musella Davide , Strati Cecilia , Lanuti Alessandro , Mastroianni Simone , Penna Stefano , Guidobaldi Andrea , Giordano Fabrizio , Petrolati Eleonora , Brown Thomas M. , Reale Andrea , Di Carlo Aldo 
Published: October 2013

AbstractNew generation photovoltaic (PV) devices such as polymer and dye sensitized solar cells (DSC) have now reached a more mature stage of development, and among their various applications, building integrated PVs seems to have the most promising future, especially for DSC devices. This new generation technology has attracted an increasing interest because of its low cost due to the use of cheap printable materials and simple manufacturing techniques, easy production, and relatively high efficiency. As for the more consolidated PV technologies, DSCs need to be tested in real operating conditions and their performance compared with other PV technologies to put into evidence the real potential. This work presents the results of a 3 months outdoor monitoring activity performed on a DSC mini‐panel made by the Dyepower Consortium, positioned on a south oriented vertical plane together with a double junction amorphous silicon (a‐Si) device and a multi‐crystalline silicon (m‐Si) device at the ESTER station of the University of Rome Tor Vergata. Good performance of the DSC mini‐panel has been observed for this particular configuration, where the DSC energy production compares favorably with that of a‐Si and m‐Si especially at high solar angles of incidence confirming the suitability of this technology for the integration into building facades. This assumption is confirmed by the energy produced per nominal watt‐peak for the duration of the measurement campaign by the DSC that is 12% higher than that by a‐Si and only 3% lower than that by m‐Si for these operating conditions. Copyright © 2013 John Wiley & Sons, Ltd.
Comparison of affective instability in borderline personality disorder and bipolar disorder using a self‐report measure
Authors: Reich D. Bradford , Zanarini Mary C. , Hopwood Christopher J. , Thomas Katherine M. , Fitzmaurice Garrett M. 
Published: October 2013

ABSTRACTBackgroundThis study used a new self‐report instrument to measure frequency and intensity of nine dimensions of affective instability, as well as the extent to which affective instability was interpersonal, in subjects endorsing elevated features of BPD and bipolar disorder.MethodSubjects were 818 students enrolled in a state university. The study used self‐report instruments to identify 21 participants with elevated features of mania and 30 subjects with significant BPD features. In addition, subjects completed a new self‐report instrument to measure affective instability, the Affective Lability Questionnaire for Borderline Personality Disorder (ALQ‐BPD).ResultsAggregate frequency and intensity scores, as well as the total scores on the ALQ‐BPD, were significantly higher for subjects with elevated borderline traits than subjects with elevated bipolar traits. Subjects with borderline traits reported significantly more frequent affective shifts on seven of nine dimensions of the ALQ‐BPD. Subjects with borderline traits reported significantly more intense affective shifts on two of nine dimensions. Both groups reported affective instability that was less than 50% reactive to interpersonal events, but subjects with borderline traits reported affective instability that was significantly more interpersonal than that reported by subjects with bipolar traits.ConclusionThe affective instability associated with symptoms of BPD and bipolar disorder has different profiles, particularly with respect to frequency. Borderline affective instability appears more interpersonal than the affective instability associated with bipolar symptoms. Copyright © 2013 John Wiley & Sons, Ltd.
Psychotropic discontinuation leading to an NMS‐like condition
Authors: Kurien Renjith , Vattakatuchery Joe John 
Published: October 2013

AbstractNeuroleptic malignant syndrome (NMS) is a potentially life‐threatening condition, which can occur as a complication in patients taking antipsychotic medications. Drs Kurien and Vattakatuchery present the case of a patient with paranoid schizophrenia who developed NMS‐like symptoms following abrupt discontinuation of clozapine and venlafaxine.
Doubly dendronized chiral polymers showing thermoresponsive properties
Authors: Li Wen , Zhang Xiacong , Zhao Xin , Zhang Xiuqiang , Zhang Afang 
Published: October 2013

ABSTRACTThe dendronization of linear polymers by dendrons with different cores and peripheries provides a convenient strategy to fuse distinct properties in one matter. By combining thermoresponsive properties with chirality, a series of doubly dendronized polymers possessing interior chiral proline units and peripheral oligoethylene glycol (OEG)‐based dendrons are synthesized and characterized. The chirality of proline moieties are varied to check potential effects on thermoresponsiveness and chiroptical properties, and the terminal groups in the OEG periphery are changed to tune the hydrophilicity of the resulting polymers. The macromonomer route is applied to obtain polymers with well‐defined structures. Free radical polymerization in bulk results in polymers with surprisingly high molar masses. Their thermally induced phase transition processes are monitored by UV–vis spectroscopy, and chiroptical properties are monitored by optical rotation measurements and circular dichroism spectroscopy. These doubly dendronized polymers show characteristic thermoresponsive behavior, and their phase transition temperatures are dominated by the peripheral structures. Polymerization accompanies weak chiral amplification, but the chirality of the proline interior contributes significantly to the thermal stability of chiroptical properties of the resulting polymers. In vitro cytotoxicity measurements are carried out to check the biocompatibility of these thermoresponsive polymers. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2013, 51, 5143–5152
Synthesis and structure–property relationships of poly(sulfone)s for anion exchange membranes
Authors: Yan Jingling , Moore Hunter D. , Hibbs Michael R. , Hickner Michael A. 
Published: October 2013

ABSTRACTMembranes based on cationic polymers that conduct anions are important for enabling alkaline membrane fuel cells and other solid‐state electrochemical devices that operate at high pH. Anion exchange membranes with poly(arylene ether sulfone) backbones are demonstrated by two routes: chloromethylation of commercially available poly(sulfone)s or radical bromination of benzylmethyl moieties in poly(sulfone)s containing tetramethylbisphenol A monomer residues. Polymers with tethered trimethylbenzyl ammonium moieties resulted from conversion of the halomethyl groups by quaternization with trimethyl amine. The water uptake of the chloromethylated polymers was dependent on the type of poly(sulfone) backbone for a given IEC. Bisphenol A‐based Udel® poly(sulfone) membranes swelled in water to a large extent while membranes from biphenol‐based Radel® poly(sulfone), a stiffer backbone than Udel, only showed moderate water uptake. The water uptake of cationic poly(sulfone)s was further reduced by synthesizing tetramethylbisphenol A and 4,4′‐biphenol‐containing poly(sulfone) copolymers where the ionic groups were clustered on the tetramethylbisphenol A residues. The conductivity of all samples scaled with the bulk water uptake. The hydration number of the membranes could be increased by casting membranes from the ionic form polymers versus converting the halomethyl form cast polymers to ionic form in the solid state. © 2013 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2013, 51, 1790–1798, 2013
Unique photoactive nanocomposites based on rhodamine 6G/polymer/montmorillonite hybrid systems
Authors: Czímerová Adriana , Jankovič Lubos , Madejová Jana , Čeklovský Alexander 
Published: October 2013

ABSTRACTAn organo‐montmorillonite (MMT) complex [poly‐diallyldimethyl(PDDA)‐Kunipia F] with various loadings of polycation was modified with a laser dye Rhodamine 6G (R6G) to obtain a photofunctional hybrid materials for monitoring of dye‐polymer‐MMT nanocomposite interactions. The polymer‐MMTs were prepared in a colloidal state via ion‐exchange reaction. The content of polycation (PDDA) in suspensions varied from 20 mg to 1000 mg per 1 g of Kunipia F (KF) MMT. Dramatic changes was observed in the structure of PPDA‐MMT containing 200–300 mg of polymer per 1 g of KF, which was the saturation point of the polycation for this system. The PDDA molecules were able to suppress the formation of nonluminescent H‐aggregates and to enhance the overall luminescence properties. The PDDA/MMT nanocomposite systems are the novel ones that have not been studied before in order to prepare highly fluorescence hybrid systems. © 2013 Wiley Periodicals, Inc. J. Polym. Sci. Part B: Polym. Phys. 2013, 51, 1672–1679
Effectiveness of Japanese SHARE model in improving Taiwanese healthcare personnel's preference for cancer truth telling
Authors: Tang Woung‐Ru , Chen Kuan‐Yu , Hsu Sheng‐Hui , Juang Yeong‐Yuh , Chiu Shin‐Che , Hsiao Shu‐Chun , Fujimori Maiko , Fang Chun‐Kai 
Published: October 2013

AbstractBackgroundCommunication skills training (CST) based on the Japanese SHARE model of family‐centered truth telling in Asian countries has been adopted in Taiwan. However, its effectiveness in Taiwan has only been preliminarily verified. This study aimed to test the effect of SHARE model‐centered CST on Taiwanese healthcare providers' truth‐telling preference, to determine the effect size, and to compare the effect of 1‐day and 2‐day CST programs on participants' truth‐telling preference.MethodFor this one‐group, pretest–posttest study, 10 CST programs were conducted from August 2010 to November 2011 under certified facilitators and with standard patients. Participants (257 healthcare personnel from northern, central, southern, and eastern Taiwan) chose the 1‐day (n = 94) or 2‐day (n = 163) CST program as convenient. Participants' self‐reported truth‐telling preference was measured before and immediately after CST programs, with CST program assessment afterward.ResultsThe CST programs significantly improved healthcare personnel's truth‐telling preference (mean pretest and posttest scores ± standard deviation (SD): 263.8 ± 27.0 vs. 281.8 ± 22.9, p < 0.001). The CST programs effected a significant, large (d = 0.91) improvement in overall truth‐telling preference and significantly improved method of disclosure, emotional support, and additional information (p < 0.001). Participation in 1‐day or 2‐day CST programs did not significantly affect participants' truth‐telling preference (p > 0.05) except for the setting subscale. Most participants were satisfied with the CST programs (93.8%) and were willing to recommend them to colleagues (98.5%).ConclusionsThe SHARE model‐centered CST programs significantly improved Taiwanese healthcare personnel's truth‐telling preference. Future studies should objectively assess participants' truth‐telling preference, for example, by cancer patients, their families, and other medical team personnel and at longer times after CST programs. Copyright © 2013 John Wiley & Sons, Ltd.
Is there a relationship between posttraumatic stress and growth after a lymphoma diagnosis?
Authors: Smith Sophia K. , Samsa Greg , Ganz Patricia A. , Zimmerman Sheryl 
Published: October 2013

AbstractObjectiveThere are conflicting empirical data regarding the relationship between posttraumatic stress (PTS) and growth (PTG) observed in cancer survivors. Clarification of this association could inform evidence‐based therapeutic recommendations to promote adjustment in survivors following a cancer diagnosis.MethodsThis cross‐sectional study employed standardized measures to examine the association between PTS and PTG in a sample of long‐term lymphoma survivors. In addition, associations between PTG and demographic, clinical and psychosocial variables were identified to inform clinical recommendations.ResultsLong‐term survivors of non‐Hodgkin lymphoma provided informed consent (n = 886; 74% response rate). Subjects averaged 10.2 years post‐diagnosis and 62.9 years of age. No significant association was found between the PTS and PTG summary scores. Several demographic and clinical variables (e.g., female gender and greater social support) were independently associated with greater PTG.ConclusionsClinicians are advised to be attentive to psychosocial needs throughout the post‐cancer diagnosis adjustment period by screening for PTS symptomatology and recognizing that survivors who report growth may also be highly distressed. Copyright © 2013 John Wiley & Sons, Ltd.
Differential proteomic profiling in human spermatozoa that did or did not result in pregnancy via IVF and AID
Authors: Zhu Yong , Wu Yibo , Jin Ke , Lu Hui , Liu Feng , Guo Yueshuai , Yan Feng , Shi Wenbo , Liu Yong , Cao Xiaorong , Hu Hongliang , Zhu Hui , Guo Xuejiang , Sha Jiahao , Li Zheng , Zhou Zuoming 
Published: October 2013

PurposeTo identify biochemical markers in men with idiopathic infertility and normal sperm counts.Experimental designWe obtained proteomic profiling proteins in human spermatozoa following successful or unsuccessful pregnancy via assisted reproductive technique (ART) using 6‐plex tandem mass tag (TMT) isobaric MS. Our study design consisted of two groups: (i) The semen of six men whose sperm resulted in a clinical pregnancy following ART and (ii) six men whose semen did not result in a clinical pregnancy following ART. The results of differentiated MS were validated by Western blotting.Results and discussionA total of 2045 proteins were detected in our cohort. Twenty‐one proteins were found to be differentially expressed (>1.2‐fold) between Group 1 and Group 2. Using the results of bioinformatics analysis and Western blotting, three proteins (A2LD1, ATP1B3, and FBXO2) were shown to have the same differential pattern (p < 0.05) that was observed in the MS analysis.Conclusions and clinical relevanceProteomics may help identity a select cohort of men with normal semen parameters and aid infertility diagnoses.
Disruption of LptA oligomerization and affinity of the LptA–LptC interaction
Authors: Schultz Kathryn M. , Feix Jimmy B. , Klug Candice S. 
Published: October 2013

AbstractThe lipopolysaccharide (LPS)‐rich outer membrane (OM) is a unique feature of Gram‐negative bacteria, and LPS transport across the inner membrane (IM) and through the periplasm is essential to the biogenesis and maintenance of the OM. LPS is transported across the periplasm to the outer leaflet of the OM by the LPS transport (Lpt) system, which in Escherichia coli is comprised of seven recently identified proteins, including LptA, LptC, LptDE, and LptFGB2. Structures of the periplasmic protein LptA and the soluble portion of the membrane‐associated protein LptC have been solved and show these two proteins to be highly structurally homologous with unique folds. LptA has been shown to form concentration dependent oligomers that stack end‐to‐end. LptA and LptC have been shown to associate in vivo and are expected to form a similar protein–protein interface to that found in the LptA dimer. In these studies, we disrupted LptA oligomerization by introducing two point mutations that removed a lysine and glutamine side chain from the C‐terminal β‐strand of LptA. This loss of oligomerization was characterized using EPR spectroscopy techniques and the affinity of the interaction between the mutant LptA protein and WT LptC was determined using EPR spectroscopy (Kd = 15 µM) and isothermal titration calorimetry (Kd = 14 µM). Kd values were also measured by EPR spectroscopy for the interaction between LptC and WT LptA (4 µM) and for WT LptA oligomerization (29 µM). These data suggest that the affinity between LptA and LptC is stronger than the affinity for LptA oligomerization.
Molecular basis of MAP kinase regulation
Authors: Peti Wolfgang , Page Rebecca 
Published: October 2013

AbstractMitogen‐activated protein kinases (MAPKs; ERK1/2, p38, JNK, and ERK5) have evolved to transduce environmental and developmental signals (growth factors, stress) into adaptive and programmed responses (differentiation, inflammation, apoptosis). Almost 20 years ago, it was discovered that MAPKs contain a docking site in the C‐terminal lobe that binds a conserved 13‐16 amino acid sequence known as the D‐ or KIM‐motif (kinase interaction motif). Recent crystal structures of MAPK:KIM‐peptide complexes are leading to a precise understanding of how KIM sequences contribute to MAPK selectivity. In addition, new crystal and especially NMR studies are revealing how residues outside the canonical KIM motif interact with specific MAPKs and contribute further to MAPK selectivity and signaling pathway fidelity. In this review, we focus on these recent studies, with an emphasis on the use of NMR spectroscopy, isothermal titration calorimetry and small angle X‐ray scattering to investigate these processes.
Metal cation controls myosin and actomyosin kinetics
Authors: Tkachev Yaroslav V. , Ge Jinghua , Negrashov Igor V. , Nesmelov Yuri E. 
Published: October 2013

AbstractWe have perturbed myosin nucleotide binding site with magnesium‐, manganese‐, or calcium‐nucleotide complexes, using metal cation as a probe to examine the pathways of myosin ATPase in the presence of actin. We have used transient time‐resolved FRET, myosin intrinsic fluorescence, fluorescence of pyrene labeled actin, combined with the steady state myosin ATPase activity measurements of previously characterized D.discoideum myosin construct A639C:K498C. We found that actin activation of myosin ATPase does not depend on metal cation, regardless of the cation‐specific kinetics of nucleotide binding and dissociation. The rate limiting step of myosin ATPase depends on the metal cation. The rate of the recovery stroke and the reverse recovery stroke is directly proportional to the ionic radius of the cation. The rate of nucleotide release from myosin and actomyosin, and ATP binding to actomyosin depends on the cation coordination number.
p62/SQSTM1 is required for cell survival of apoptosis‐resistant bone metastatic prostate cancer cell lines
Authors: Chang Megan A. , Morgado Micaela , Warren Curtis R. , Hinton Cimona V. , Farach‐Carson Mary C. , Delk Nikki A. 
Published: October 2013

AbstractBACKGROUNDBone marrow stromal cell (BMSC) paracrine factor(s) can induce apoptosis in bone metastatic prostate cancer (PCa) cell lines. However, the PCa cells that escape BMSC‐induced apoptosis can upregulate cytoprotective autophagy.METHODSC4‐2, C4‐2B, MDA PCa 2a, MDA PCa 2b, VCaP, PC3, or DU145 PCa cell lines were grown in BMSC conditioned medium and analyzed for mRNA and/or protein accumulation of p62 (also known as sequestome‐1/SQSTM1), Microtubule‐associated protein 1 light chain 3B (LC3B), or lysosomal‐associated membrane protein 1 (LAMP1) using quantitative polymerase chain reaction (QPCR), Western blot, or immunofluorescence. Small interfering RNA (siRNA) was used to determine if p62 is necessary PCa cell survival.RESULTSBMSC paracrine signaling upregulatedp62mRNA and protein in a subset of the PCa cell lines. The PCa cell lines that were insensitive to BMSC‐induced apoptosis and autophagy induction had elevated basalp62mRNA and protein. In the BMSC‐insensitive PCa cell lines, siRNA knockdown ofp62was cytotoxic and immunostaining showed peri‐nuclear clustering of autolysosomes. However, in the BMSC‐sensitive PCa cell lines,p62siRNA knockdown was not appreciably cytotoxic and did not affect autolysosome subcellular localization.CONCLUSIONSA pattern emerges wherein the BMSC‐sensitive PCa cell lines are known to be osteoblastic and express the androgen receptor, while the BMSC‐insensitive PCa cell lines are characteristically osteolytic and do not express the androgen receptor. Furthermore, BMSC‐insensitive PCa may have evolved a dependency on p62 for cell survival that could be exploited to target and kill these apoptosis‐resistant PCa cells in the bone.Prostate 74:149–163, 2014. © 2013 Wiley Periodicals, Inc.
Crystal structure of Mycobacterium tuberculosis CarD, an essential RNA polymerase binding protein, reveals a quasidomain‐swapped dimeric structural architecture
Authors: Kaur Gundeep , Dutta Dipak , Thakur Krishan Gopal 
Published: October 2013

ABSTRACTMycobacterium tuberculosis (Mtb) CarD is an essential transcriptional regulator that binds RNA polymerase and plays an important role in reprogramming transcription machinery under diverse stress conditions. Here, we report the crystal structure of CarD at 2.3 Å resolution, that represents the first structural description of CarD/CdnL‐Like family of proteins. CarD adopts an overall bi‐lobed structural architecture where N‐terminal domain resembles ‘tudor‐like’ domain and C‐terminal domain adopts a novel five helical fold that lacks the predicted leucine zipper structural motif. The structure reveals dimeric state of CarD resulting from β‐strand swapping between the N‐terminal domains of each individual subunits. The structure provides crucial insights into the possible mode(s) of CarD/RNAP interactions. Proteins 2014; 82:879–884. © 2013 Wiley Periodicals, Inc.
Intramolecular isopeptide but not internal thioester bonds confer proteolytic and significant thermal stability to the S. pyogenes pilus adhesin Spy0125
Authors: Walden Miriam , Crow Allister , Nelson Miles D. , Banfield Mark J. 
Published: October 2013

ABSTRACTStreptococcus pyogenes and other Gram‐positive bacterial pathogens present long macromolecular filaments known as pili on their surface that mediate adhesion and colonization. These pili are covalent polymers, assembled by sortases. Typically, they comprise a putative adhesin at their tip, a backbone subunit present in multiple copies and a basal subunit that is covalently anchored to the peptidoglycan layer of the cell surface. The crystal structures of pilin subunits revealed the presence of unusual covalent linkages in these proteins, including intramolecular isopeptide and internal thioester bonds. The intramolecular isopeptide bonds in backbone pilins are important for protein stability. Here, using both the wild‐type protein and a set of mutants, we assessed the proteolytic and thermal stability of the S. pyogenes pilus tip adhesin Spy0125, in the presence and absence of its intramolecular isopeptide and internal thioester bonds. We also determined a crystal structure of the internal thioester bond variant Spy0125Cys426Ala. We find that mutations in the intramolecular isopeptide bonds compromise the stability of Spy0125. Using limited proteolysis and thermal denaturation assays, we could separate the contribution of each intramolecular isopeptide bond to Spy0125 stability. In contrast, mutation in the internal thioester bond had a lesser effect on protein stability and the crystal structure is essentially identical to wild type. This work suggests that the internal thioester in Spy0125, although having a minor contributory role, is not required for protein stability and must have a different primary function, most likely mediating a covalent interaction with host cell ligands. Proteins 2014; 82:517–527. © 2013 The Authors Proteins: Structure, Function, and Bioinformatics Published by Wiley Periodicals, Inc.