bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced expression of microRNA-100 confers unfavorable prognosis in patients with bladder cancer
Authors: Wang Sheng , Xue Sheng , Dai Yuanqing , Yang Jianfu , Chen Zhijun , Fang Xiwu , Zhou Wensheng , Wu Wei , Li Qingwen 
Published: November 2012

Abstract

Objective
MicroRNA-100 (miR-100) has been demonstrated to be downregulated in bladder cancer tissues, and enforced expression of this miRNA may inhibit cell growth and colony formation of human bladder cancer 5637 cells in vitro. However, the clinical significance of miR-100 in human bladder cancer has not yet been elucidated. Thus, the aim of this study was to investigate the diagnostic and prognostic values of miR-100 in this disease.

Methods
Expression levels of miR-100 in 126 pairs of bladder cancer and adjacent normal tissues were detected by TaqMan real-time quantitative RT-PCR assay. In order to determine its prognostic value, overall survival (OS) and progression-free survival (PFS) were evaluated using the Kaplan-Meier method, and multivariate analysis was performed using the Cox proportional hazard analysis.

Results
Expression levels of miR-100 in bladder cancer tissues were significantly lower than those in adjacent normal tissues (mean expression level: 2.6 ± 1.2 vs. 3.9 ± 1.5, P < 0.001). When categorized into low vs. high expression, low miR-100 expression was negatively associated with the stage (P = 0.01), the recurrence (P = 0.008), the progression (P = 0.01), and the death (P < 0.001) of patients with bladder cancer. Moreover, low miR-100 expression clearly predicted poorer PFS (P = 0.001) and OS (P < 0.001). In the multivariate analysis, low miR-100 expression was an independent prognostic factor for both PFS (P = 0.01) and OS (P = 0.008).

Conclusion
Our data offer the convincing evidence that miR-100 may play an important role in the progression of bladder cancer and that the reduced expression of this miRNA may be independently associated with shorter PFS and OS of patients, suggesting that miR-100 might be a potential marker for further risk stratification in the treatment of this cancer.

Virtual slides
The virtual slides’ for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/1105483419841671
An UPLC-MS/MS method for highly sensitive high-throughput analysis of phytohormones in plant tissues
Authors: Balcke Gerd Ulrich , Handrick Vinzenz , Bergau Nick , Fichtner Mandy , Henning Anja , Stellmach Hagen , Tissier Alain , Hause Bettina , Frolov Andrej 
Published: November 2012

AbstractBackgroundPhytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and modulating plant reactions targeted against pathogens and herbivores, as well as resistance to abiotic stress (drought, UV-irradiation and mechanical wounding). These factors induce dramatic changes in phytohormone biosynthesis and transport leading to rapid local and systemic stress responses. Understanding of underlying mechanisms is of principle interest for scientists working in various areas of plant biology. However, highly sensitive, precise and high-throughput methods for quantification of these phytohormones in small samples of plant tissues are still missing.ResultsHere we present an LC-MS/MS method for fast and highly sensitive determination of jasmonates, abscisic and salicylic acids. A single-step sample preparation procedure based on mixed-mode solid phase extraction was efficiently combined with essential improvements in mobile phase composition yielding higher efficiency of chromatographic separation and MS-sensitivity. This strategy resulted in dramatic increase in overall sensitivity, allowing successful determination of phytohormones in small (less than 50 mg of fresh weight) tissue samples. The method was completely validated in terms of analyte recovery, sensitivity, linearity and precision. Additionally, it was cross-validated with a well-established GC-MS-based procedure and its applicability to a variety of plant species and organs was verified.ConclusionThe method can be applied for the analyses of target phytohormones in small tissue samples obtained from any plant species and/or plant part relying on any commercially available (even less sensitive) tandem mass spectrometry instrumentation.
Experimental determination of the oral bioavailability and bioaccessibility of lead particles
Authors: Deshommes Elise , Tardif Robert , Edwards Marc , Sauvé Sébastien , Prévost Michèle 
Published: November 2012

Abstract

In vivo estimations of Pb particle bioavailability are costly and variable, because of the nature of animal assays. The most feasible alternative for increasing the number of investigations carried out on Pb particle bioavailability is in vitro testing. This testing method requires calibration using in vivo data on an adapted animal model, so that the results will be valid for childhood exposure assessment. Also, the test results must be reproducible within and between laboratories. The Relative Bioaccessibility Leaching Procedure, which is calibrated with in vivo data on soils, presents the highest degree of validation and simplicity. This method could be applied to Pb particles, including those in paint and dust, and those in drinking water systems, which although relevant, have been poorly investigated up to now for childhood exposure assessment.
Hemoperitoneum presenting with the use of a topical hemostatic agent in oocyte retrieval: a case report
Authors: Chatrian Amélie , Vidal Clémentine , Equy Véronique , Hoffmann Pascale , Sergent Fabrice 
Published: November 2012

Abstract

Introduction
Hemoperitoneum may occur from an ovarian puncture point after oocyte retrieval.

Case presentation
We report a case of massive hemoperitoneum following transvaginal ultrasound-guided oocyte retrieval in a 33-year-old Caucasian woman. The bleeding required emergency laparoscopy because of active bleeding from the ovarian puncture point. Hemostasis was very difficult to achieve, and traditional operative procedures were not efficient. The only way to stop the bleeding was by using an absorbable fibrinogen and thrombin sealant sponge, which was applied around the ovary. During laparoscopy three pints of packed red blood were administered. No specific alteration of screening coagulation tests was found one month later.

Conclusions
Hemostasis can be very difficult to achieve with traditional operative procedures. Topical hemostatic agents can be useful to preserve the ovary wherever possible.
Colostrum supplementation protects against exercise - induced oxidative stress in skeletal muscle in mice
Authors: Appukutty Mahenderan , Radhakrishnan Ammu Kutty , Ramasamy Kalavathy , Ramasamy Rajesh , Abdul Majeed Abu Bakar , Noor Mohd Ismail , Safii Nik Shanita , Koon Poh Bee , Chinna Karuthan , Haleagrahara Nagaraja 
Published: November 2012

Abstract

Background
This study examined the effects of bovine colostrum on exercise –induced modulation of antioxidant parameters in skeletal muscle in mice. Adult male BALB/c mice were randomly divided into four groups (control, colostrum alone, exercise and exercise with colostrum) and each group had three subgroups (day 0, 21 and 42). Colostrum groups of mice were given a daily oral supplement of 50 mg/kg body weight of bovine colostrum and the exercise group of mice were made to exercise on the treadmill for 30 minutes per day. Total antioxidants, lipid hydroperoxides, xanthine oxidase and super oxide dismutase level was assayed from the homogenate of hind limb skeletal muscle.

Results
Exercise—induced a significant oxidative stress in skeletal muscles as evidenced by the elevated lipid hydroperoxides and xanthine oxidase levels. There was a significant decrease in skeletal muscle total antioxidants and superoxide dismutase levels. Daily colostrum supplement significantly reduced the lipid hydroperoxides and xanthine oxidase enzyme level and increased the total antioxidant levels in the leg muscle.

Conclusion
Thus, the findings of this study showed that daily bovine colostrum supplementation was beneficial to skeletal muscle to reduce the oxidant-induced damage during muscular exercise.
Hydroxyapatite bioactivated bacterial cellulose promotes osteoblast growth and the formation of bone nodules
Authors: Tazi Neftaha , Zhang Ze , Messaddeq Younès , Almeida-Lopes Luciana , Zanardi Lisinéia M , Levinson Dennis , Rouabhia Mahmoud 
Published: November 2012

AbstractThe goal of this study was to investigate the feasibility of bacterial cellulose (BC) scaffold to support osteoblast growth and bone formation. BC was produced by culturingAcetobacter xylinumsupplemented with hydroxyapatite (HA) to form BC membranes (without HA) and BC/HA membranes. Membranes were subjected to X-ray photoelectron spectroscopy (XPS) analysis to determine surface element composition. The membranes were further used to evaluate osteoblast growth, alkaline phosphatase activity and bone nodule formation. BC was free of calcium and phosphate. However, XPS analysis revealed the presence of both calcium (10%) and phosphate (10%) at the surface of the BC/HA membrane. Osteoblast culture showed that BC alone was non-toxic and could sustain osteoblast adhesion. Furthermore, osteoblast adhesion and growth were significantly (p ≤0.05) increased on BC/HA membranes as compared to BC alone. Both BC and BC/HA membranes improved osteoconductivity, as confirmed by the level of alkaline phosphatase (ALP) activity that increased from 2.5 mM with BC alone to 5.3 mM with BC/HA. BC/HA membranes also showed greater nodule formation and mineralization than the BC membrane alone. This was confirmed by Alizarin red staining (ARS) and energy dispersive X-ray spectroscopy (EDX). This work demonstrates that both BC and BC/HA may be useful in bone tissue engineering.
Gestaltung von AAL-Lösungen als sozio-technische Systeme: Selbstgesteuerte Alltagsunterstützung
Authors: Prilla Michael , Herrmann Thomas 
Published: November 2012

Zusammenfassung
Bei der Gestaltung und Umsetzung von Ambient Assisted Living (AAL) wird zu wenig beachtet, dass es in der Praxis um sozio-technische Lösungen geht, bei denen eine Integration zwischen Nutzern, organisatorischen Prozessen und eingesetzter Technik erfolgskritisch ist. Dieser Beitrag zeigt am Beispiel des Projekts service4home Herausforderungen und Lösungen für AAL aus sozio-technischer Perspektive auf.
Living Labs als Forschungsinstrument für Ambient Assisted Living Technologien
Authors: Rashid Asarnusch , Reichelt Christian , Röll Natalie , Zentek Tom 
Published: November 2012

Zusammenfassung
Seit über vier Jahren setzt das FZI Forschungszentrum Informatik die Living Lab Methode zur Forschung an Ambient Assisted Living (AAL) Technologien ein. Ca. 15 wissenschaftliche Mitarbeiter aus unterschiedlichen Projekten und Disziplinen arbeiten hier zusammen, um innovative Assistenzsysteme für ältere bzw. körperlich und kognitiv eingeschränkte Menschen zu entwickeln. Das Living Lab erwies als sehr hilfreich, um zum einen techni-sche Möglichkeiten aufzuzeigen und zum anderen Benut-zerakzeptanzanalysen und Usability Tests von AAL Tech-nologien durchzuführen. Dieser Beitrag beschreibt den theoretischen Rahmen der Living Lab Methodik und die Erfahrungen mit der Umsetzung des Living Lab zur For-schung an AAL Technologien.
Multiple Acetabular Revisions in THA - Poor Outcome Despite Maximum Effort
Authors: Bischel O , Seeger JB , Krüge M , Bitsch BG 
Published: November 2012

A consecutive series of 52 acetabular revisions was evaluated retrospectively. Inclusion criteria for all patients were at least one former exchange of the acetabular component. Reconstruction was performed with reliable techniques and implants other than extensively porous coated device (e.g. tantalum). The mean follow up was 5.63 (0.01-14.05) years. Cumulative survival at 14.05 years with removal of the acetabular component due to aseptic loosening or the worst case criterion (removal of the acetabular component for any cause and/or lost to follow-up) as the end point was 66.38 (95 % C.-I.: 47.80-84.96) % and 58.42 (95 % C.-I.: 41.01-75.83) %, respectively. The cumulative survival rate with mechanical failure of the acetabular reconstruction as the endpoint was significantly lower in patients with two or more previous revisions in comparison to those with only one former procedure (log rank test: p=0,0112 respectively). The mean Merle d’Aubignée-score improved from 7.3 (0-14) preoperatively to 10.6 (0-17) points at latest follow up examination.Survival of acetabular reconstructions with common techniques and implants is decreasing with the number of previous revisions. This may cause major concerns with regard to the rising number of patients needing repeated revisions. Maximizing durability of primary THA, precise preoperative planning as well as improved techniques and implants for revision may decrease this problem in the long term.
Postglacial vegetation history as recorded from the subalpine Lake Ribno (NW Rila Mts), Bulgaria
Authors: Tonkov Spassimir , Bozilova Elissaveta , Possnert Goran 
Published: November 2012

AbstractA pollen analysis conducted on a 600 cm core from Lake Ribno (2184 m) in the Northwestern Rila Mountains, supplemented by 13 radiocarbon dates, has revealed the basic stages in its postglacial vegetation dynamics. The lateglacial vegetation, composed of Artemisia, Chenopodiaceae and Poaceae, with stands of Pinus and Juniperus-Ephedra shrubland, dominated in the stadials and partly retreated during the Bølling/Allerød interstadial (14700–12900 cal. yrs. BP). The afforestation in the early Holocene (11600–7800 cal. yrs. BP) started with pioneer Betula forests, with groups of Pinus and Juniperus at mid-high altitudes, and Quercus forests with Tilia, Ulmus, Fraxinus, Corylus below the birch zone. A coniferous belt composed of Pinus
sylvestris, P. peuce and Abies was shaped under the conditions of a more humid climate (7800–5800 cal. yrs. BP). The last trees that invaded the study area were Fagus after 4300 cal. yrs. BP and Picea abies after 3400 cal. yrs. BP. Evidence for destructive changes in the vegetation and indications of agricultural and stock-breeding activities (pollen of Triticum, Secale, Plantago lanceolata, Rumex, Juglans) was continuously recorded since the Late Bronze Age (3400–3200 cal. yrs. BP). The postglacial vegetation history in the Northwestern Rila Mountains demonstrated close similarities with that of the Northern Pirin Mountains.
The effect of summer shading on flower bud morphogenesis in apricot (Prunus armeniaca L.)
Authors: Bartolini Susanna , Viti Raffaella , Andreini Lucia 
Published: November 2012

AbstractThe aim of this investigation was to assess whether imposed summer shading treatments in apricot (Prunus armeniaca L.) can affect the main phenological phases related to the floral morphogenesis (floral differentiation, xylogenesis), flower bud growth and quality in terms of bud capacity to set fruit. Experimental trials were carried out on fully-grown trees of ‘San Castrese’ and ‘Stark Early Orange’ cultivars characterized by different biological and agronomical traits to which shadings were imposed in July and August. Histological analysis was carried out from summer onwards in order to determine the evolution of floral bud differentiation, and the acropetal progression of primary xylem differentiation along the flower bud axis. Periodical recordings to evaluate the bud drop, blooming time, flowering and fruit set rates were performed also. These shade treatments determined a temporary shutdown of floral differentiation, slowed xylem progression up to the resumption of flower bud growth and a reduced entity of flowering and fruit set. These events were particularly marked in ‘San Castrese’ cultivar, which is well known for its adaptability to different climatic conditions. These findings suggest that adequate light penetration within the canopy during the summer season could be the determining factor when defining the qualitative traits of flower buds and their regular growth, and ultimately to obtain good and constant crops.
The effect of genotype on a barley scutella culture. Histological aspects
Authors: Ślesak Halina , Góralski Grzegorz , Pawłowska Helena , Skucińska Barbara , Popielarska-Konieczna Marzena , Joachimiak Andrzej 
Published: November 2012

AbstractCereals are known to be recalcitrant to the induction of morphogenesis in vitro and the majority of the methods used are callus-mediated and species/genotype-dependent. In the present investigation, a method of morphogenesis induction from immature scutella of selected barley cultivars was used, and particular attention was paid to histology in the initial stages of the regeneration process in order to confirm whether it occurs directly or indirectly (via callus formation). The length of the period from inoculating scutella on the medium to obtaining plantlets depended on the cultivar and the individual scutellum of the barley and varied between 2.5–4 months. The regeneration efficiency and viability of barley scutella was revealed to be highly genotype dependent. The average number of regenerated plants per regenerating scutellum was highest in the case of cv Granal (3.7). A histological analysis of the cultured explants showed both non-morphogenic and morphogenic callus formation. Two types of indirect morphogenetic response were observed: organogenesis (shoot bud formation) and somatic embryogenesis. This is the first report concerning an analysis of in vitro regeneration from immature scutella of barley cultivars (Stratus, Ryton, Granal and Binal).
Roles of iron and copper salts for controlling morphology of silver nanostructures
Authors: Jia Changchao , Yang Ping , Zhang Lipeng 
Published: November 2012

Abstract
Various silver nanostructures were fabricated by means of ethylene glycol reduction of silver nitrate together with poly (vinyl pyrrolidone) through a polyol-thermal method. Variable valency metal ions (iron and copper) affected the morphologies of these nanostructures. Because of enhanced reduction ability compared with Cu+ ions, Fe2+ ions resulted in Ag+ being reduced to Ag0 efficiently. Regular silver nanowires were prepared using copper salt, while quasi-flag types of silver nanostructures were obtained in the case of iron salt. Quasi-flag types of silver nanostructures were also created successfully using both CuCl2 and FeCl2 in the reaction system. Furthermore, the diameter and morphologies of silver nanostructures were adjusted by changing the amount of poly (vinyl pyrrolidone) and CuCl2. A mechanism is proposed to understand the morphology evolution of silver nanostructures.
Hydrothermal synthesis of nanowires, nanobelts, and nanotubes of vanadium oxides from one reaction system
Authors: Li Zhihua , Zhang Yanwu , Shi Zhaohui 
Published: November 2012

Abstract
Nanowires, nanobelts, and nanotubes of vanadium oxides have been fabricated using the hydrothermal method with surfactants in one reaction system. The nanowires, nanobelts and nanotubes of vanadium oxides can be synthesized selectively by adjusting and controlling the reaction conditions such as surfactants selected, reaction temperature and time, etc. The as-prepared one-dimentional nanomaterials were characterized by means of X-ray diffraction, field emission scanning electron microscopy, transmission electron microscopy, electron diffraction, and high-resolution transmission electronmicroscopy. The reaction mechanism is also explained according to the experimental data.
Bone marrow concentrate for autologous transplantation in minipigs
Authors: Herten M. , Sager M. , Benga L. , Fischer J. C. , Jäger M. , Betsch M. , Wild M. , Hakimi M. , Jungbluth P. , Grassmann J. P. 
Published: November 2012

SummaryAutologous bone marrow plays an increasing role in the treatment of bone, cartilage and tendon healing disorders. Cell-based therapies display promising results in the support of local regeneration, especially therapies using intra-operative one-step treatments with autologous progenitor cells. In the present study, bone marrow-derived cells were concentrated in a point-of-care device and investigated for their mesenchymal stem cell (MSC) characteristics and their osteogenic potential.Bone marrow was harvested from the iliac crest of 16 minipigs. The mononucleated cells (MNC) were concentrated by gradient density centrifugation, cultivated, characterized by flow cytometry and stimulated into osteoblasts, adipocytes, and chondrocytes. Cell differentiation was investigated by histological and immunohistological staining of relevant lineage markers. The proliferation capacity was determined via colony forming units of fibroblast and of osteogenic alkaline-phosphatase-positive-cells.The MNC could be enriched 3.5-fold in nucleated cell concentrate in comparison to bone marrow. Flow cytometry analysis revealed a positive signal for the MSC markers. Cells could be differentiated into the three lines confirming the MSC character. The cellular osteogenic potential correlated significantly with the percentage of newly formed bone in vivo in a porcine metaphyseal long-bone defect model.This study demonstrates that bone marrow concentrate from minipigs display cells with MSC character and their osteogenic differentiation potential can be used for osseous defect repair in autologous transplantations.
En-bloc femoral cement removal after failure of cemented total hip replacement in two dogs
Authors: Song J. , Sheehy J. G. , Dyce J. 
Published: November 2012

SummaryIn two dogs with periprosthetic infection after total hip replacement, the femoral cement mantle was retrieved by proximal extraction without an invasive osteotomy or cortical fenestration. En-bloc femoral cement removal was performed by injection of polymethylmethacrylate cement into the central mantle void left after stem removal, and by threading a positive profile pin into the newly injected cement. Once the PMMA had polymerized, the pin was withdrawn with the entire mantle attached. This technique should be considered in patients with circumferential deterioration of the femoral bone-cement interface in which the diameter of the femoral isthmus would not obstruct withdrawal of the cement mantle.
Quelle langue...
Authors: Mc Loughlin Mary 
Published: November 2012

Résumé Cet écrit tente de soulever quelques questions autour de la langue, maternelle et étrangère, et les différentes places que chacune occupe. Quelles sont les difficultés rencontrées dans le passage de l’une à l’autre ? Que faire de l’espace entre-deux, l’espace de l’intraduisible ? La situation analytique est-elle différente en fonction de la langue parlée ?
L'inconscient et le temps
Authors: Fierens Christian 
Published: November 2012

Résumé L’inconscient en lui n’apparaît pas. Il est intemporel. L’inconscient ne peut passer, dans le temps de l’analyse, que moyennant le « signifiant ». On explicite l’opération de ce dernier primo par la subversion du sujet, secundo par la coupure impliquée dans la hâte de conclure et tertio par l’absence de tout message.