bluuebunny/crossref_metadata_2025_split huggingface dataset:
Advancing Small‐Molecule‐Based Chemical Biology with Next‐Generation Sequencing Technologies
Authors: Anandhakumar Chandran , Kizaki Seiichiro , Bando Toshikazu , Pandian Ganesh N. , Sugiyama Hiroshi 
Published: November 2014

AbstractNext‐generation‐sequencing (NGS) technologies enable us to obtain extensive information by deciphering millions of individual DNA sequencing reactions simultaneously. The new DNA‐sequencing strategies exceed their precursors in output by many orders of magnitude, resulting in a quantitative increase in valuable sequence information that could be harnessed for qualitative analysis. Sequencing on this scale has facilitated significant advances in diverse disciplines, ranging from the discovery, design, and evaluation of many small molecules and relevant biological mechanisms to maturation of personalized therapies. NGS technologies that have recently become affordable allow us to gain in‐depth insight into small‐molecule‐triggered biological phenomena and empower researchers to develop advanced versions of small molecules. In this review we focus on the overlooked implications of NGS technologies in chemical biology, with a special emphasis on small‐molecule development and screening.
Reversible Assembly and Disassembly of Receptor‐Decorated Gold Nanoparticles Controlled by Ion Recognition
Authors: Aydogan Abdullah , Lee Gawon , Lee Chang‐Hee , Sessler Jonathan L. 
Published: November 2014

AbstractThe controlled assembly of randomly dispersed colloidal particles can provide access to materials with advanced optical and electronic properties while providing fundamental insights into self‐assembly processes in nature and nanotechnology. Typically, self‐assembled nanoparticles are prepared by exploiting electrostatic interactions, lithographic techniques, and covalently linked molecular scaffolds. This results in static morphologies that cannot be disassembled easily. On the other hand, having access to systems that can be assembled or disassembled in a controlled manner could allow for in‐depth understanding of the nanoparticles as well as rational control over the morphology and fundamental properties of the resulting constructs. If the changes in aggregation are induced by a specific external chemical stimulus, it could also permit the development of new chemosensors. Here we demonstrate the reversible assembly and disassembly of gold nanoparticles achieved by modulating the noncovalent interactions between surface‐bound calix[4]pyrroles and added bis‐imidazolium cations. We also demonstrate the use of these nanoparticles in the selective sensing of anions.
Unusual Chemoselective RhII‐Catalysed Transformations of α‐Diazocarbonyl Piperidine Cores
Authors: Bonetti Andrea , Beccalli Egle , Caselli Alessandro , Clerici Francesca , Pellegrino Sara , Gelmi Maria Luisa 
Published: November 2014

AbstractThe reactivity of various α‐diazocarbonyl piperidine scaffolds, characterised by an increased molecular complexity, was tested with various RhII catalysts. The structure of the starting reagent is of relevance to the synthetic results. An unexpected dimerisation took place, starting from the simple piperidine scaffold, to give the hexahydrotetrazine ring system. Products derived from a nitrogen ylide intermediate or aromatic substitution (1,3,4,5‐tetrahydro‐2,5‐methanobenzo[c]azepine and 1,2,3,3a‐tetrahydrocyclopenta[de]isoquinolin‐4(5 H)‐one rings, respectively) were obtained from tetrahydroisoquinoline derivatives. The chemoselectivity of the reaction could be controlled by the choice of starting reagent, RhII catalyst and the reaction conditions. Finally, it was found that the azepino heterocycle could coordinate to the catalyst to give new RhII complexes.
Evaluation of targets for color calibrating digital images from an optical bright‐field transmission microscope
Authors: Wei Hong , Brill Michael H. , Park Taeyoung 
Published: November 2014

AbstractColor calibration of digital microscope images of stained biological slides may use a special slide (called a calibration slide) with known color samples that are usually filters. To evaluate quantitatively how well the calibration has been done by a particular system, another set of different known samples is needed, with transmittance spectra that are typical of stained biological material. A slide with such colors comprises what is called a reference slide. In this article, a method is described for selecting the colors for such a slide. A reference slide was created by this prescription and then used to evaluate two embodiments of a color management system for several microscope and illumination combinations. It was found that a set of 20 reference colors spans the gamut of colors produced by a particular kind of stain. Using the reference slide, Datacolor's ChromaCal™ system performance was quantitatively evaluated. The performance is robust to change of microscope and illumination and does not degrade much when a tungsten light is increased in intensity (hence in correlated color temperature) with only one initial calibration at a single color temperature followed by automatic white balancing of a traditional sort. © 2014 Wiley Periodicals, Inc. Col Res Appl, 40, 577–584, 2015
Control of Spatial Organization of Electrospun Fibers in a Carbon Felt for Enhanced Bioelectrode Performance
Authors: Both Engel Adriana , Cherifi Aziz , Bechelany Mikhael , Tingry Sophie , Cornu David 
Published: November 2014

AbstractElectrospun carbon fiber electrodes showing high performance for bio‐electrochemical applications were developed. Easy to handle and manipulate aligned and unaligned carbon fibers with a mean diameter of (330±50) nm were synthesized through an electrospinning technique. Electrical resistivity measurements, which are a challenge that has not been much explored in the case of fibrous materials, were realized through two different techniques, and a study of contact resistances between electrical clips and the carbon fibers was performed. To target the creation of a bioelectrode, carbon fibers were characterized electrochemically by cyclic voltammetry. After being modified with the enzyme laccase, its response to oxygen electroreduction was studied. Aligned fibers present a cathodic current that is 30 % higher than that of randomly distributed fibers. Overall, the results show that aligned fibers are more appropriate for bio‐electrochemical applications when exploiting anisotropic spatial organization.
Highly Functionalized Biaryls via Suzuki–Miyaura Cross‐Coupling Catalyzed by Pd@MOF under Batch and Continuous Flow Regimes
Authors: Pascanu Vlad , Hansen Peter R. , Bermejo Gómez Antonio , Ayats Carles , Platero‐Prats Ana E. , Johansson Magnus J. , Pericàs Miquel À. , Martín‐Matute Belén 
Published: November 2014

AbstractA diverse set of more than 40 highly functionalized biaryls was synthesized successfully through the Suzuki–Miyaura cross‐coupling reaction catalyzed by Pd nanoparticles supported in a functionalized mesoporous MOF (8 wt % Pd@MIL‐101(Cr)‐NH2). This could be achieved under some of the mildest conditions reported to date and a strong control over the leaching of metallic species could be maintained, despite the presence of diverse functional groups and/or several heteroatoms. Some of the targeted molecules are important intermediates in the synthesis of pharmaceuticals and we clearly exemplify the versatility of this catalytic system, which affords better yields than currently existing commercial procedures. Most importantly, Pd@MIL‐101‐NH2 was packed in a micro‐flow reactor, which represents the first report of metallic nanoparticles supported on MOFs employed in flow chemistry for catalytic applications. A small library of 11 isolated compounds was created in a continuous experiment without replacing the catalyst, demonstrating the potential of the catalyst for large‐scale applications.
Growth and Properties of Amorphous Erbium‐doped Aluminum‐yttrium Oxide Films Deposited by Aerosol‐UV‐Assisted MOCVD
Authors: Salhi Rached , Jimenez Carmen , Deschanvres Jean‐Luc , Maâlej Ramzi , Fourati Mohieddine 
Published: November 2014

Erbium‐doped yttrium‐aluminum oxide films (Er:Y2O3‐Al2O3) are deposited by aerosol‐assisted metal‐organic (AA‐MO)CVD. The effects of the humidity of the carrier gas and UV assistance on their structure and optical properties during the deposition are investigated as a function of the substrate temperature and the aluminum mole fraction (Al2O3 mol.‐%) in the liquid solution. The effect of substrate temperature is studied for a constant Al concentration of 33.33 mol.‐% of Al‐acac in the solution. The maximum deposition rates are reached under lower air humidity and with UV assistance in a surface temperature range between 350 and 460 °C. Nevertheless, as‐deposited Er:Al2O3‐Y2O3 films show a very low organic contamination when depositions take place under high air humidity and with UV assistance. The film composition is strongly dependent on air humidity, showing a very high aluminum content when working with a high humidity of the carrier gas, and yttrium‐rich when working with a low humidity of the carrier gas. The refractive index of Er:Al2O3‐Y2O3 films under these conditions is relatively high, reaching 1.76 when deposited at 460 °C.The effect of composition is studied at a substrate temperature of 410 °C. The effect on film composition when varying the aluminum mole fraction in the liquid solution is studied. The most influential parameter is the high air humidity, which induces stronger variation on the layer composition for the same liquid composition.
Combination of Diacerhein and Antiepileptic Drugs After Local Peripheral, and Oral Administration in the Rat Formalin Test
Authors: Zúñiga‐Romero Angel , Ponce‐Chávez Martha Karina , Gauthereau‐Torres Marcia Yvette , Ortega‐Varela Luis Fernando 
Published: November 2014

Abstract

Preclinical Research

The present study was designed to evaluate the possible antinociceptive interaction between diacerhein and some antiepileptic drugs (carbamazepine, topiramate and gabapentin) on formalin‐induced nociception. Diacerhein, each of the antiepileptics or a fixed dose‐ratio combination of these drugs was assessed after local peripheral and oral administration in rats. lsobolographic analyses were used to define the interaction between drugs. Diacerhein, antiepileptic drugs (carbamazepine, topiramate and gabapentin) or their combinations yielded a dose‐dependent antinociceptive effect when administered by both routes. Theoretical ED30 values for the combination estimated from the isobolograms were obtained as follows: diacerhein‐carbamazepine (85.99 ± 7.07 μg/paw; 56.53 ± 4.56 mg/kg po), diacerhein‐topiramate (197.97 ± 22.90 μg/paw; 13.06 ± 2.44 mg/kg po) and diacerhein‐gabapentin (96.87 ± 17.73 μg/paw; 17.90 ± 4.70 mg/kg p.o.) for the local peripheral and oral administration routes, respectively. These values were significantly higher than the experimentally obtained ED30 values: diacerhein‐carbamazepine (49.33 ± 3.37 μg/paw; 35.49 ± 7.91 mg/kg po), diacerhein‐topiramate (133.00 ± 39.10 μg/paw; 8.87 ± 1.46 mg/kg po) and diacerhein‐gabapentin (70.98 ± 14.73 μg/paw; 10.95 ± 3.23 mg/kg po). The combinations produced their antinociceptive effects without motor impairment in the rotarod test indicating synergistic interactions with a good side effect profile.
Engaging Local Private and Public Actors in Biodiversity Conservation: The role of Agri‐Environmental schemes and Ecological fiscal transfers
Authors: dos Santos Rui Ferreira , Antunes Paula , Ring Irene , Clemente Pedro 
Published: November 2014

AbstractBiodiversity conservation policies have relied on regulatory approaches, such as the designation of protected areas, which have generally been insufficient to ensure long‐term conservation. This is partially explained by the mismatch between those who bear the costs of and those who benefit from conservation. While local actors, private and public, face management and opportunity costs due to conservation policies, the benefits reach beyond local borders. In this paper, we argue that a successful biodiversity conservation policy mix should include economic instruments directed at public and private local stakeholders. These instruments should be mutually reinforcing and target decision processes regarding land‐use zoning and land management practices. To illustrate our argument, we focus on European agri‐environmental measures (directed at private landowners) and ecological fiscal transfers (for local governments). We describe each of these instruments and derive main lessons from their implementation. Finally, we present proposals for linking them to reinforce their individual contributions to the overall goal of halting biodiversity loss, taking the Portuguese case as an example. Copyright © 2014 John Wiley & Sons, Ltd and ERP Environment
Clinical trials update from the European Society of Cardiology meeting 2014: PARADIGM‐HF, CONFIRM‐HF, SIGNIFY, atrial fibrillation, beta‐blockers and heart failure, and vagal stimulation in heart failure
Authors: Clark Andrew L. , Pellicori Pierpaolo 
Published: November 2014

AbstractThis article provides an overview of trials relevant to the pathophysiology, prevention, and treatment of heart failure, presented at the European Society of Cardiology meeting held in Barcelona in autumn 2014. Trials reported here include PARADIGM‐HF (LCZ696 versus enalapril in heart failure), CONFIRM‐HF (treatment of iron deficiency in heart failure), and SIGNIFY (ivabradine in patients with stable coronary artery disease). In addition, we discuss recent developments in the treatment of atrial fibrillation and the lack of benefit with the use of beta‐blockers in these patients. Finally, the article describes recent advances in the use of vagal stimulation in patients with heart failure. © 2014 The Authors. ESC Heart Failure published by John Wiley & Sons Ltd on behalf of the European Society of Cardiology.
Sexual function in patients supported with left ventricular assist device and with heart transplant
Authors: Hasin Tal , Jaarsma Tiny , Murninkas Daniel , Setareh‐Shenas Saman , Yaari Victoria , Bar‐Yosef Simona , Medalion Benjamin , Gerber Yariv , Ben‐Gal Tuvia 
Published: November 2014

AbstractAimsSexual dysfunction is common among patients with heart failure (HF) and considered an important hamper to quality of life. While implantation of left ventricular assist device (LVAD) may prolong and improve life in advanced HF, limited data are available on its impact on sexual function. The aim of this study is to evaluate sexual function in LVAD patients and compare this with patients after heart transplantation (HTx).Methods and resultsSexual activity and satisfaction of stable patients with durable LVAD or after HTx were evaluated using a validated questionnaire and visual analogue scale from 0 to 10. Data were collected from 31 patients (mean age 59 ± 12 years, 87% male), 17 after HTx and 14 with LVAD. Pleasure or satisfaction with sex was significantly higher in HTx patients (P = 0.0005). In total, 29% LVAD patients and 71% HTx patients reported content with sexual activity. Recalled satisfaction with sex life pre‐operation was comparable between the groups. During support, satisfaction with sex life using visual analogue scale was 7.6 ± 3.1 for HTx versus 3.9 ± 4.0 for LVAD patients (P = 0.017). In total, 11 LVAD patients (79%) reported specific problems in sexual function including erectile dysfunction or vaginal dryness (8, 57%); problems with the LVAD, cable, or batteries (5, 36%); problems with orgasm (4,29%); and other problems such as fear of injury, feeling depressed, partner issues, self‐image, and pain (1, 7% each).ConclusionSexual dysfunction occurs in patients with LVAD support and may be more prominent than after HTx. Problems limiting sexual function related to physiological, psychological, and equipment merit consideration during follow‐up.
Metal Mixture Modeling Evaluation project: 2. Comparison of four modeling approaches
Authors: Farley Kevin J. , Meyer Joseph S. , Balistrieri Laurie S. , De Schamphelaere Karel A. C. , Iwasaki Yuichi , Janssen Colin R. , Kamo Masashi , Lofts Stephen , Mebane Christopher A. , Naito Wataru , Ryan Adam C. , Santore Robert C. , Tipping Edward 
Published: November 2014

Abstract

As part of the Metal Mixture Modeling Evaluation (MMME) project, models were developed by the National Institute of Advanced Industrial Science and Technology (Japan), the US Geological Survey (USA), HDR|HydroQual (USA), and the Centre for Ecology and Hydrology (United Kingdom) to address the effects of metal mixtures on biological responses of aquatic organisms. A comparison of the 4 models, as they were presented at the MMME workshop in Brussels, Belgium (May 2012), is provided in the present study. Overall, the models were found to be similar in structure (free ion activities computed by the Windermere humic aqueous model [WHAM]; specific or nonspecific binding of metals/cations in or on the organism; specification of metal potency factors or toxicity response functions to relate metal accumulation to biological response). Major differences in modeling approaches are attributed to various modeling assumptions (e.g., single vs multiple types of binding sites on the organism) and specific calibration strategies that affected the selection of model parameters. The models provided a reasonable description of additive (or nearly additive) toxicity for a number of individual toxicity test results. Less-than-additive toxicity was more difficult to describe with the available models. Because of limitations in the available datasets and the strong interrelationships among the model parameters (binding constants, potency factors, toxicity response parameters), further evaluation of specific model assumptions and calibration strategies is needed. Environ Toxicol Chem 2015;34:741–753. © 2014 SETAC
Examination of the prevalence of stimulus overselectivity in children with ASD
Authors: Rieth Sarah R. , Stahmer Aubyn C. , Suhrheinrich Jessica , Schreibman Laura 
Published: November 2014

AbstractMany individuals with autism spectrum disorders (ASD) display stimulus overselectivity, wherein a subset of relevant components in a compound stimulus controls responding, which impairs discrimination learning. The original experimental research on stimulus overselectivity in ASD was conducted several decades ago; however, interventions for children with ASD now typically include programming to target conditional discriminations in ways that might minimize the prevalence of stimulus overselectivity. The present study assessed 42 children who had been diagnosed or educationally identified with ASD using a discrimination learning assessment. Of these 42 children, 19% displayed overselective responding, which is a lower percentage than that seen in early research. Possible explanations for this decreased percentage, implications for intervention, and future directions for research are discussed.
Electrospun poly(ε‐caprolactone)‐based skin substitutes: In vivo evaluation of wound healing and the mechanism of cell proliferation
Authors: Augustine Robin , Dominic Edwin Anto , Reju Indu , Kaimal Balarama , Kalarikkal Nandakumar , Thomas Sabu 
Published: November 2014

AbstractIn the present study, we have fabricated electrospun poly(ε‐caprolactone)‐based membranes, characterized and studied the in vivo cell migration and proliferation and wound healing activity. Moreover, we did not seed any cells prior to the animal implantation and we could observe excellent fibroblast attachment and cell proliferation. Further full thickness excision wound on guinea pig completely healed within 35 days. We could reach in an assumption that the enhanced cell proliferation and wound healing might be due to the surface degradation of the polymer under physiological conditions and the formation of functional groups like hydroxyl and carboxyl groups that promoted cell proliferation in a cell adhesion protein mediated mechanism. This study is a novel tissue engineering concept for the reconstruction of a damaged tissue without the in vitro cell seeding and proliferation prior to the in vivo implantation. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1445–1454, 2015.
Femoral Volumetric Bone Density, Geometry, and Strength in Relation to 25-Hydroxy Vitamin D in Older Men
Authors: Martin Elizabeth N , Haney Elizabeth M , Shannon Jackie , Cauley Jane A , Ensrud Kristine E , Keaveny Tony M , Zmuda Joseph M , Orwoll Eric S , Harrison Stephanie Litwack , Marshall Lynn M 
Published: November 2014

ABSTRACT

Low serum 25-hydroxy vitamin D (25(OH)D) concentrations are associated with increased hip fracture risk and decreased femoral areal bone mineral density (BMD) among elderly men. Structural dimensions of the proximal femur and volumetric BMD in cortical and trabecular compartments are also associated with hip fracture risk. However, associations of volumetric BMD or structural dimensions with serum 25(OH)D concentrations among older men remain unclear. In a random sample of 1608 men aged ≥65 years from the Osteoporotic Fractures in Men Study (MrOS), baseline serum 25(OH)D concentrations were measured by liquid chromatography/mass spectrometry assays. Femoral neck geometry and volumetric BMD derived from quantitative computed tomography included integral, cortical, and trabecular volumetric BMD; cross-sectional area; integral and cortical volume; and cortical volume as a percent of integral volume. We studied 888 men with vitamin D, parathyroid hormone (PTH), femoral neck geometry, and BMD measures. Whole-bone femoral strength and load-strength ratio from finite element (FE) analysis were also available for 356 men from this sample. Multivariable linear regression was used to estimate least square means of each femoral measure within quartiles of 25(OH)D adjusted for age, race, body mass index, height, latitude, and season of blood draw. Tests of linear trend in the means were performed across increasing quartile of serum 25(OH)D levels. Mean cortical volume (p trend = 0.006) and cortical volume as a percent of integral volume (p trend &lt; 0.001) increased across increasing quartile of 25(OH)D level. However, overall femoral neck size (area and integral volume) did not vary by 25(OH)D level. Femoral neck volumetric BMD measures increased in a graded manner with higher 25(OH)D levels (p trend &lt; 0.001). Femoral strength, but not load-strength ratio, increased with increasing 25(OH)D. Adjustment for PTH did not materially change these associations. We conclude that in older men, higher levels of endogenous 25(OH)D may increase whole-bone strength by increasing femoral volumetric BMD and cortical volume. © 2014 American Society for Bone and Mineral Research.
Partial Geometric Difference Families
Authors: Nowak Kathleen , Olmez Oktay , Song Sung Y. 
Published: November 2014

AbstractWe introduce the notion of a partial geometric difference family as a variation on the classical difference family and a generalization of partial geometric difference sets. We study the relationship between partial geometric difference families and both partial geometric designs and difference families, and show that partial geometric difference families give rise to partial geometric designs. We construct several infinite families of partial geometric difference families using Galois rings and the cyclotomy of Galois fields. From these partial geometric difference families, we generate a list of infinite families of partial geometric designs and directed strongly regular graphs.