bluuebunny/crossref_metadata_2025_split huggingface dataset:
A RUPTURED NONCOMMUNICATING RUDIMENTARY HORN PREGNANCY: A CASE REPORT
Authors: Dejen Getaneh , Amenu Demisew 
Published: July 2024

Pregnancy in the rudimentary horn is rare and carries grave consequences for the mother. Here, we report a case of 26-year G3P2 with 17 weeks gestation who presented with lower abdominal pain. Ultrasound showed hemoperitoneum with suspicion of abdominal ectopic pregnancy. Intraoperatively the diagnosis of ruptured left rudimentary horn pregnancy was made. The fetus and placenta were removed followed by excision of the horn with ipsilateral salpingectomy done.
Keywords: Ruptured rudimentary horn pregnancy, Unicornuate uterus, uterine anomalies
PREVALENCE OF PRE-ECLAMPSIA AND ECLAMPSIA AND MATERNO-FETAL OUTCOMES AT A HEALTH FACILTY IN DELTA STATE, NIGERIA
Authors: Clement Aghoja Ogheneovo , Ahwinahwi Ufuoma Shalom , Ahwinahwi Ufuoma Shalom , Arute John Edjophe , Arute John Edjophe , Oliseyenum Delight Weluche 
Published: July 2024

BACKGROUND: Globally, pre-eclampsia and eclampsia complicate up to 4.6% and 1.4% of pregnancies respectively but disproportionally account for nearly 18% of all maternal death worldwide, with an estimated 62,000 to 77,000 deaths per year.
OBJECTIVE: This study determined the prevalence of pre-eclampsia and eclampsia, and compared outcomes.
METHODS: This was a retrospective cross-sectional study utilizing the case files of women admitted to the maternity ward of the obstetrics and gynaecology unit in Central Hospital, Ughelli from 1st August 2020 to 30th July 2022. Socio-demographic characteristics, maternal and fetal complications and outcomes, prevalence rate of preeclampsia and eclampsia, were presented as frequencies and percentages.
RESULTS: Of the 6291 mothers which were delivered of their babies in the maternity ward of the obstetrics and gynaecology department, 120(1.9%) were diagnosed of pre-eclampsia and eclampsia; only 108 which had complete documentation were used for the study. Age range of the participants was 16-45years, the majority, 88(81.5%) were married, had secondary education, 70(64.8%) and higher; 42(38.9%) were nulliparous at admission and 67(62%) were unbooked at the antenatal clinic. Fifty –three (49.1%) had preeclampsia while 55(50.9%) had eclampsia. Majority, 107(99.1%) were discharged home alive and 75(69.4%) had no maternal complications. Number of fetuses delivered were 114, 95 (83.3%) were born alive, 68(59.6%) weighed less than 2500g, and 91( 95.8%) neonates had a good APGAR score at the 5th minute.
CONCLUSION: The prevalence of pre-eclampsia and eclampsia was low, however there was high rates of perinatal deaths and occurrence of unfavourable materno-fetal complications.
Prevalence and predictors of ovarian hyper stimulation syndrome at Center fertility and repro-ductive medicine at Public IVF center, Ethiopia
Authors: Ansa Meseret , Negash Mustefa , Wondafrash Mekitie , Teshome Abel 
Published: July 2024

Abstract
Background
Infertility affects fifteen percent of couples who wish to conceive. The mainstay of artificial reproductive technologies is in vitro fertilization and embryo transfer (IVF-ET), in which aspirated Oocytes are fertilized, followed by the trans cervical replacement of an embryo(s) into the uterine cavity.However, the practice of assisted reproductive technology is loaded with short- and long-term complications. Complications may occur during the course of stimulation, ovum pickup, or embryo transfer. Ample studies are done on in vitro fertilization, but the frequency and importance of complications of IVF in low-resource setups where the treatment itself is not widely available are poorly known.
Objective
This study aimed to determine the rate of ovarian hyper stimulation syndrome and associated factors among women who underwent controlled ovarian stimulation and IVF in a public IVF center.
Methodology
A simple random sampling technique was used to review the medical records of women who have undergone ovarian stimulation and IVF treatment at the Saint Paul’s Hospital Center for Reproductive Medicine and IVF.
Results
A total of 428 clients had controlled ovarian stimulation and IVF. The mean age of the participants was33yrs among 428 couples who had IVF, majority 245 (57%) had IVF for female factor infertility followed by male factor infertility 89(20%) and unexplained causes 53(12%).
The incidence of OHSS in our IVF population was 19(4.4%) out of 428 women. out of the 22 patients with OHSS 17 (87%) developed mild and moderate and the rest 2 out of 22(10 %) had severe OHSS. The odd of developing OHSS was 6 times among those with PCOS, OR 5.78 CI (1.19, 28.22), p value of 0.03.
Conclusions and recommendations
The overall rate of ovarian hyper stimulation syndrome is higher in our IVF population.
Emphasis should be given on counseling and risk minimizing measures when women withPCOS
Key words: IVF, Complications, Ovarian hyperstimulation syndrome
A retrospective analysis of maternal and pregnancy outcomes following PMTCT maternal cohort registration in a displaced setting.
Authors: Ngwibete Atenchong , Oluwatobi Popoola Victor , Oluwasola Timothy 
Published: July 2024

Purpose: This study assessed the maternal and pregnancy outcomes following PMTCT cohort registration.
Design: A retrospective analysis of HIV-positive pregnant women’s outcomes following enrollment in the Prevention of Mother-to-Child Transmission (PMTCT) maternal cohort register between January 2019 and December 2021 in FSP Daudu.
Method: Using a checklist, data was collected from the folders and PMTCT maternal cohort register. Measures of interest included sociodemographic characteristics, maternal outcomes, and pregnancy outcomes.
Findings: Of 223 identified HIV-positive pregnant women, 201 were enrolled in the program. However, only 189 maternal records met the inclusion criteria. Entry into the PMTCT program was mainly during the antenatal period, between 26 and 30 years of age with a mean gestational age of 15.2 weeks. Only 5.9% of the women reported facility delivery, and up to 70% had over 4 PMTCT follow-up visits before delivery (?2 = 6.825, P = 0.03). The Retention rate among the cohort was 98.4%, with 62% of the women being active throughout the program and over 86% having a live birth. Bivariate analysis suggested that aside from maternal age, similar factors affected maternal and pregnancy outcomes.
Conclusions The active involvement and engagement of an HIV-positive pregnant woman in care can improve their health outcomes in times of displacement.
Clinical evidence: Active follow-up and documentation constitute an effective strategy to improve PMTCT maternal retention in care and improve maternal outcomes. Further support by engaging community health workers in service delivery will create positive outcomes in the PMTCT program.
Keywords: PMTCT, HIV/AIDs, Maternal health, Pregnancy.
Influence of Certain Biostimulants on the Vegetative Growth of Annual Moringa (Moringa oleifera. Lam)
Authors: F. M Huria, , Shaaban E. H. , Gaber M. K. 
Published: July 2024

Due to the importance of moringa leaves which can help improve the dietary diversity and quality of households that need to improve their nutritional intake, being a potential alternate source of protein, and for the sake of increasing its growth and yield, this study is determine the effect of some biofertilizers, amino acids and seaweed extract on vegetative growth of moringa seedlings As well as, to find out the best fertilization treatment to improve the vegetative growth of Moringa and reducing the intensive use of chemical fertilizers. This study was conducted in the Experimental Farm of the Faculty of Agriculture (Saba-Basha), Alexandria University, Egypt, during two consecutive seasons of 2020 and 2021, to study the effect of biofertilizers, amino acids, and seaweed extract and its combinations on the growth of Moringa (Moringa oleifera, L.). The experiment was designed as split-split plot design it included 27 treatments with three replicates. The results revealed that the highest significant values of vegetative growth as plant height (171.8 cm), stem length (87.30 cm), stem diameter (3.49 cm), leaves fresh weight (70.61 g), leaves dry weight (13.35g), number of leaves per plant (72.03), and number of leaves per shoot (54.41) were achieved when the trees were soil bio-fertilized and sprayed with the investigated amino acid 700 mg/l + seaweed extract 700 mg/l + cerealine (T26) for both seasons of study. While the number of branches per plant (15.28), shoot fresh weight (138.1g), and shoot dry weight (24.07g) were concerned, the highest values were obtained when the plants were treated with soil bio-fertilization (Phosphorine), sprayed with amino acids, and seaweed extract, as the abovementioned treatments, amino acid 700 mg/l + seaweed extract 700 mg/l + Phosphorine (T25), for both seasons of study compared to the other treatments and control.
Net CO2 surface emissions at Bern, Switzerland inferred from ambient observations of CO2, δ(O2/N2), and 222Rn using a customized radon tracer inversion
Authors: van der Laan S. , van der Laan‐Luijkx I. T. , Zimmermann L. , Conen F. , Leuenberger M. 
Published: January 2014

AbstractThe 222Radon tracer method is a powerful tool to estimate local and regional surface emissions of, e.g., greenhouse gases. In this paper we demonstrate that in practice, the method as it is commonly used, produces inaccurate results in case of nonhomogeneously spread emission sources, and we propose a different approach to account for this. We have applied the new methodology to ambient observations of CO2 and 222Radon to estimate CO2 surface emissions for the city of Bern, Switzerland. Furthermore, by utilizing combined measurements of CO2 and δ(O2/N2) we obtain valuable information about the spatial and temporal variability of the main emission sources. Mean net CO2 emissions based on 2 years of observations are estimated at (11.2 ± 2.9) kt km−2 a−1. Oxidative ratios indicate a significant influence from the regional biosphere in summer/spring and fossil fuel combustion processes in winter/autumn. Our data indicate that the emissions from fossil fuels are, to a large degree, related to the combustion of natural gas which is used for heating purposes.
Linking organic carbon sedimentation, burial efficiency, and long‐term accumulation in boreal lakes
Authors: Ferland Marie‐Eve , Prairie Yves T. , Teodoru Cristian , del Giorgio Paul A. 
Published: January 2014

AbstractCarbon (C) storage in lakes is now recognized as a significant sink of C at a global scale, but the pathways that lead to this storage remain poorly understood. In this study, we attempt to reconstruct and connect the processes that lead to long‐term C accumulation in boreal lakes. These include the rate of particulate organic C (POC) sedimentation in the water column and sediment metabolism operating at a temporal scale of weeks to months, organic C accumulation in the top sediment layers integrated over scales of tens of years, and long‐term organic C burial in lake sediment integrated over hundreds to thousands of years. The sinking POC flux was tenfold higher than the short‐term sediment C accumulation rates in all systems, and we found no direct relationship between this downward C flux and either the short‐term or long‐term C accumulation rates. However, the resulting C burial efficiency (which ranged from 5 to 62%) was strongly related to lake shape, which ultimately constrains the time freshly deposited material that is exposed to oxygen and thereby regulates the fraction of the carbon sinking flux that is mineralized back to the atmosphere or permanently buried in the sediments. Small and deep lakes act as more efficient C sinks than large and flat lakes. We also show that long‐term burial rates are nearly identical to current centennial‐scale accumulation rates and that therefore, little degradation occurs after a few decades. Sediment C storage tends to be small (<5%) relative to lake C emissions, but that this balance is also strongly related to lake morphometry.
Dual salt mixtures in mixed mode chromatography with an immobilized tryptophan ligand influence the removal of aggregated monoclonal antibodies
Authors: Vajda Judith , Mueller Egbert , Bahret Eva 
Published: January 2014

AbstractIn downstream processing of monoclonal antibodies, proper aggregate removal is crucial. Mixed mode ligands such as immobilized tryptophan have been developed to satisfy the need for efficient removal of antibody aggregates. However, method development for mixed mode applications is complicated, since protein binding and elution can be modulated by an increased set of parameters. In the current study, we investigate the effect of different dual salt mixtures on mixed mode chromatography using TOYOPEARL MX‐Trp‐650M resin, with respect to the dynamic binding capacity, resolution and monomer purity of two different humanized immunoglobulins. Binding capacities varying by more than 50% were observed for different salt mixtures. Furthermore, antibody monomer and aggregate resolution deviated by 30% for different salt mixtures and linear gradient elution. Similar trends were obtained using an immobilized carboxymethyl ligand for the same set of experiments, but the overall resolution was lower. Less kosmotropic salt systems emphasize the electrostatic binding of the relatively hydrophobic mAbs and reduce hydrophobic attraction to a selectivity‐determining constraint. Kosmotropic salts such as citrate appear to cause dominating hydrophobic interactions in protein adsorption that hinder electrostatic protein‐ligand interactions. This effect may depend on the ionic and hydrophobic site distribution of a protein. The data presented here are important for the further improvement of downstream processing of therapeutic monoclonal antibodies.
A xenogeneic‐free bioreactor system for the clinical‐scale expansion of human mesenchymal stem/stromal cells
Authors: dos Santos Francisco , Campbell Andrew , Fernandes‐Platzgummer Ana , Andrade Pedro Z. , Gimble Jeffrey M. , Wen Yuan , Boucher Shayne , Vemuri Mohan C. , da Silva Cláudia L. , Cabral Joaquim M.S. 
Published: January 2014

ABSTRACTThe large cell doses (>1 × 106 cells/kg) used in clinical trials with mesenchymal stem/stromal cells (MSC) will require an efficient production process. Moreover, monitoring and control of MSC ex‐vivo expansion is critical to provide a safe and reliable cell product. Bioprocess engineering approaches, such as bioreactor technology, offer the adequate tools to develop and optimize a cost‐effective culture system for the rapid expansion of human MSC for cellular therapy. Herein, a xenogeneic (xeno)‐free microcarrier‐based culture system was successfully established for bone marrow (BM) MSC and adipose tissue‐derived stem/stromal cell (ASC) cultivation using a 1L‐scale controlled stirred‐tank bioreactor, allowing the production of (1.1 ± 0.1) × 108 and (4.5 ± 0.2) × 107 cells for BM MSC and ASC, respectively, after 7 days. Additionally, the effect of different percent air saturation values (%Airsat) and feeding regime on the proliferation and metabolism of BM MSC was evaluated. No significant differences in cell growth and metabolic patterns were observed under 20% and 9%Airsat. Also, the three different feeding regimes studied—(i) 25% daily medium renewal, (ii) 25% medium renewal every 2 days, and (iii) fed‐batch addition of concentrated nutrients and growth factors every 2 days—yielded similar cell numbers, and only slight metabolic differences were observed. Moreover, the immunophenotype (positive for CD73, CD90 and CD105 and negative for CD31, CD80 and HLA‐DR) and multilineage differentiative potential of expanded cells were not affected upon bioreactor culture. These results demonstrated the feasibility of expanding human MSC from different sources in a clinically relevant expansion configuration in a controlled microcarrier‐based stirred culture system under xeno‐free conditions. The further optimization of this bioreactor culture system will represent a crucial step towards an efficient GMP‐compliant clinical‐scale MSC production system. Biotechnol. Bioeng. 2014;111: 1116–1127. © 2014 Wiley Periodicals, Inc.
Characterization of time‐course morphological features for efficient prediction of osteogenic potential in human mesenchymal stem cells
Authors: Matsuoka Fumiko , Takeuchi Ichiro , Agata Hideki , Kagami Hideaki , Shiono Hirofumi , Kiyota Yasujiro , Honda Hiroyuki , Kato Ryuji 
Published: January 2014

ABSTRACTHuman bone marrow mesenchymal stem cells (hBMSCs) represents one of the most frequently applied cell sources for clinical bone regeneration. To achieve the greatest therapeutic effect, it is crucial to evaluate the osteogenic differentiation potential of the stem cells during their culture before the implantation. However, the practical evaluation of stem cell osteogenicity has been limited to invasive biological marker analysis that only enables assaying a single end‐point. To innovate around invasive quality assessments in clinical cell therapy, we previously explored and demonstrated the positive predictive value of using time‐course images taken during differentiation culture for hBMSC bone differentiation potential. This initial method establishes proof of concept for a morphology‐based cell evaluation approach, but reveals a practical limitation when considering the need to handle large amounts of image data. In this report, we aimed to scale‐down our proposed method into a more practical, efficient modeling scheme that can be more broadly implemented by physicians on the frontiers of clinical cell therapy. We investigated which morphological features are critical during the osteogenic differentiation period to assure the performance of prediction models with reduced burden on image acquisition. To our knowledge, this is the first detailed characterization that describes both the critical observation period and the critical number of time‐points needed for morphological features to adequately model osteogenic potential. Our results revealed three important observations: (i) the morphological features from the first 3 days of differentiation are sufficiently informative to predict bone differentiation potential, both activities of alkaline phosphatase and calcium deposition, after 3 weeks of continuous culture; (ii) intervals of 48 h are sufficient for measuring critical morphological features; and (iii) morphological features are most accurately predictive when early morphological features from the first 3 days of differentiation are combined with later features (after 10 days of differentiation). Biotechnol. Bioeng. 2014;111: 1430–1439. © 2014 Wiley Periodicals, Inc.
Evaluation of high‐capacity cation exchange chromatography for direct capture of monoclonal antibodies from high‐titer cell culture processes
Authors: Tao Yinying , Ibraheem Aladein , Conley Lynn , Cecchini Douglas , Ghose Sanchayita 
Published: January 2014

ABSTRACTAdvances in molecular biology and cell culture technology have led to monoclonal antibody titers in excess of 10 g/L. Such an increase can pose concern to traditional antibody purification processes due to limitations in column hardware and binding capacity of Protein A resins. Recent development of high capacity cation exchangers can make cation exchange chromatography (CEX) a promising and economic alternative to Protein A capture. This work investigates the feasibility of using CEX for direct capture of monoclonal antibodies from high titer cell culture fluids. Two resin candidates were selected from seven newer generation cation exchangers for their higher binding capacity and selectivity. Two monoclonal antibodies with widely differing pI values were used to evaluate the capability of CEX as a platform capture step. Screening of loading pH and conductivity showed both resins to be capable of directly capturing both antibodies from undiluted cell culture fluid. At appropriate acidic pH range, product loading of over 65 g/L resin was achieved for both antibodies. A systematic design of experiment (DOE) approach was used to optimize the elution conditions for the CEX step. Elution pH showed the most significant impact on clearance of host cell proteins (HCPs). Under optimal conditions, HCP reduction factors in the range of 9–44 were achieved on the CEX step based on the pI of the antibody. Apart from comparing CEX directly to Protein A as the capture method, material from either modality was also processed through the subsequent polishing steps to compare product quality at the drug substance level. Process performance and product quality was found to be acceptable using the non‐affinity based process scheme. The results shown here present a cheaper and higher capacity generic capture method for high‐titer antibody processes. Biotechnol. Bioeng. 2014;111: 1354–1364. © 2014 Wiley Periodicals, Inc.
Pharmacokinetics of dronedarone in rat using a newly developed high‐performance liquid chromatographic assay method
Authors: Bin Jardan Yousef A. , Gabr Raniah Q. , Brocks Dion R. 
Published: January 2014

ABSTRACTA sensitive and selective high‐performance liquid chromatographic method for the determination of dronedarone in rat plasma was developed. Dronedarone was extracted using one‐step liquid–liquid extraction. The separation of dronedarone was accomplished using a C18 analytical column. The mobile phase was composed of a combination of monobasic potassium phosphate and acetonitrile. The UV detection was at 254 nm for ethopropazine, the internal standard, and after its elution, changed to 290 nm for dronedarone detection. The total analytical run time was 20 min. Mean recovery was >80%; the assay had excellent linear relationships (>0.999) between peak height ratios and plasma concentrations; the lower limit of quantification 25 was ng/mL, based on 100 μL of rat plasma. Accuracy and precision were <18% over the concentration range of 25–500 ng/mL. The assay was applied successfully to the measurement of dronedarone plasma concentrations in rats given the drug orally. Copyright © 2014 John Wiley & Sons, Ltd.
Uterine cervix cancer treatment at Radiumhemmet: 90 years' experience. Time trends of age, stage, and histopathology distribution
Authors: Hellman Kristina , Hellström Ann‐Cathrin , Pettersson B. Folke 
Published: January 2014

AbstractSince the introduction of screening programs for cervical cancer (CC) the incidence has decreased and CC is discovered at an earlier stage. The purpose of this study was to analyze time trends in age, stage, and histopathology over a 90‐year period and to our knowledge this is the largest single institutional series in the literature of invasive cervical carcinoma (CC) cases. This is a retrospective study comprising 18,472 women treated for CC from 1914 until 2004 at Radiumhemmet, Stockholm. The material is part of the international CC statistics published since 1937 in the League of Nations' Annual Reports, and since 1958 under the patronage of International Federation of Gynecology and Obstetrics (FIGO). During the 90‐year study period, the annual number of cases treated increased to over 400 up until 1965, after which there was a gradual drop to less than 100 cases in 2004. A pronounced shift toward earlier stages at diagnosis was noted. The mean age at diagnosis increased in all stages, predominantly in advanced stages. A reduction in squamous cell carcinoma (SCC) cases and a sixfold increase in the proportion of adenocarcinoma (AC) cases were observed. The mean age at diagnosis for squamous and AC cases shifted after 1970, when the SCC cases ultimately became 3 years older than the AC cases in contrast to around 1950 when they were 3 years younger than the AC cases. The changes in the distribution by age, stage, and histopathology during this 90‐year period are probably associated with: improved social conditions and increased access to health care, the introduction of screening programs for CC in the 1960s, and a change in the risk factors for CC (changed sexual behavior, introduction of contraceptive pills, and changed smoking habits).