bluuebunny/crossref_metadata_2025_split huggingface dataset:
TOPICAL APPLICATION OF ETHYL ETHER TO RECURRENT HERPES SIMPLEX
Authors: Takagaki Kenji , Jidoi Joji 
Published: April 2015

ABSTRACTIn order to evaluate the effect of ethyl ether application to recurrent herpes simplex, recurrence intervals were checked on patients who had been followed up for over 3 years. Typical recurrent herpes simplex cases were selected and topical ether applications were made with ether soaked cotton swabs for 10 minutes several times a day for two days. Results revealed that topical ether application remarkably prolonged the recurrence interval. In this regard, ether application was effective in the treatment of recurrent herpes simplex.
MULTIPLE LARVAL TICK INFESTATION OF MAN
Authors: Fujiwara Kunihiko , Ono Tomomichi , Kawashima Kenjiro 
Published: April 2015

ABSTRACTA 53‐year‐old Japanese male forest officer suffered from pains which seemed to be insect bites. Examination revealed a patient with more than 10 pustule‐like lesions which subsequently proved to be larval A. testudinarium infestations. All the ticks were removed with a pair of forceps. The remaining erythematous lesions were treated with 0.25% hydrocortisone clotamiton ointment. Pains disappeared soon. A diagnosis of tick infestation should be made before the tick is removed, in order to prevent severe effects from allowing the mouth parts of the tick to remain, which occasionally results in inflammation and granulation tissue formation, and also to make a good preparation of the tick for correct identification.
AN ELECTRON MICROSCOPIC STUDY OF LIPID DROPLETS IN THE NORMAL HUMAN SEBACEOUS GLAND
Authors: Niizuma Kan 
Published: April 2015

ABSTRACTLipid droplet ultrastructure in the normal human sebaceous gland was studied by the conventional method, the lipid retention procedure, and the tannic acid fixation method. The results strongly supported the views that (i) lipid droplets originate from the Golgi apparatus, and (ii) the lipid droplets do not mature by fusion of neighbouring droplets but as a result of direct participation of the smooth endoplasmic reticulum. (iii) A tri‐laminar true limiting membrane was clearly detected around all lipid droplets in certain exceptional acini, but was not otherwise detected in mature sebaceous acini.
ULTRASTRUCTURE OF INCONTINENTIA PIGMENTI ACHROMIANS, WITH SPECIAL REFERENCE TO MELANOCYTES AND NERVE ENDINGS
Authors: Morohashi Masaaki , Maeda Tetsuo , Takahashi Shozo , Igarashi Ryoichi 
Published: April 2015

ABSTRACTStudies of a new Japanese case of incontinentia pigmenti achromians were made by electron microscopy.At the periphery of the depigmented lesions, the melanocytes had several subcellular abnormalities; i.e., vacuolization of the cytoplasm, aggregation of melanosomes, and autophagic vacuoles.Many non‐specific basal dendritic cells could be identified as non‐pigment producing melanocytes. The depigmentation in this disease seemed to have been caused by both melanocyte degeneration and inactivation.Numerous nerve endings were seen in close contact with the basal lamina of the epidermis or even within the epidermis.Direct contacts between melanocytes and free nerve endings were presumed when a direct continuity between the Schwann cell basal lamina of the nerve ending and the basal lamina of melanocytes was observed.It was further observed in this study that nerve endings could be found associated with either normal or degenerated melanocytes. Apparently normal melanocytes in the lesion may not be entirely normal in function.
ATYPICAL FIBROXANTHOMA OF THE SKIN A HISTOLOGICAL AND ELECTRON MICROSCOPIC STUDY
Authors: Mihara Motoyuki , Kanbe Naoto , Shimao Shuhei , Nakakuki Shigeya 
Published: April 2015

ABSTRACTA histological and electron microscopic case study of an atypical fibroxanthoma (AFX) of the skin was reported. The lesion developed on the left lower limb of a 23‐year‐old woman. Histologically, the lesion showed three zonal arrangements: spotty, small necrotic foci filled with erythrocytes in the central zone, pleomorphic and atypical cells in the intermediate zone; and interlacing collagenous bundles with storiform patterns in the peripheral zone. Tumor cells identified by electron microscopy were divided into undifferentiated mesenchymal, fibroblast‐like, histiocyte‐like and xanthoma cells. This suggested, histogenetically, that tumor cells of AFX of the skin may be fibrohistiocytic in nature. Furthermore, lipid droplets in xanthoma cells were classified into two types: smooth, oval or round shapes without a limiting membrane; irregular shapes with a limiting membrane.
Km OF TYROSINASE IN MICROSOMES IS HIGHER THAN THAT IN MELANOSOMES
Authors: Tomita Yasushi , Hariu Akiko , Mizuno Chikako , Seiji Makoto 
Published: April 2015

ABSTRACTKm values of tyrosinase solubilized from melanosomes and microsomes from Harding‐Passey mouse melanomas, consisting of rough and smooth surface membranes were found to be 0.6 mM and 1.3 mM, respectively. The maximum reaction velocities of the two enzymes from the melanosomes and microsomes were similar. In comparison with the tyrosine level in human plasma, reported to be approximately 0.06 mM, the Km value for microsomal tyrosinase is very high. Therefore it is likely that the tyrosinase reaction occurs at a extremely low level in the smooth and rough membranes of the melanocyte. This may be one possible reason why melanin formation does not take place in vivo in rough and smooth surface membranes in the melanocytes, despite the fact that tyrosinase activity has been detected in microsomes in vitro after isolation from melanoma tissue.
COMPARATIVE STUDY OF THE SENSITIVITY OF MOUSE DORSAL ROOT GANGLION AND CEREBELLUM TO HERPES SIMPLEX VIRUS IN VITRO
Authors: Matsubara Motoo , Ueda Keiichi , Yasuno Hirokazu , Maruo Michiru , Yoshioka Akira 
Published: April 2015

ABSTRACTTo study the effect of Herpes simplex virus (HSV) on nervous tissue, we examined (1) the possibility of establishment of latent infection in vitro, (2) the replication of HSV in mouse dorsal root ganglion and cerebellar cultures and (3) the effect of anti‐HSV rabbit serum on the maintenance of both cultures. Although both cultures without antibody were finally destroyed after inoculation, replication of HSV in cerebellar cultures was much higher than in the ganglion in both medium and tissue (P<0.005). Infected cultures treated with anti‐HSV antibody (CF: ×128) could survive longer in nerve cells of ganglion than in those of cerebellum.These results suggest that the ganglion can resist HSV infection and this makes latent infection possible.
POSTIRRADIATION MALIGNANT FIBROUS HISTIOCYTOMA
Authors: Okazaki Michiharu , Inoue Shouhei , Ogata Katsumi , Narita Hiromi 
Published: April 2015

ABSTRACTA fatal case of postirradiation malignant fibrous histiocytoma was reported, with a review of postirradiation sarcoma in the Japanese literature.
PENETRATION OF TOPICAL CORTICOSTEROIDS THROUGH HUMAN EPIDERMIS
Authors: Osamura Hiromi 
Published: April 2015

ABSTRACTThe penetration of hydrocortisone, hydrocortisone 17‐butyrate 21‐propionate, betamethasone, betamethasone 17‐valerate, and clobetasol 17‐propionate through separated human epidermis was compared. The amount of each topical corticosteroid which was released on the skin and penetrated to saline was greater in ethanol solution than propylene glycol solution. The addition of water to the ethanol increased the release of some corticosteroids. The lipid solubility of each corticosteroid correlated well with skin penetration; however, the polarity of each corticosteroid was inversely proportional to skin penetration. The results of the blanching phenomenon caused by the application of these corticosteroids and of their skin penetration suggested to us that the local pharmacological activity of each topical corticosteroid, rather than their skin penetration, related well to their clinical effectiveness.
SECRETORY IgA IN SKIN AND THE ADJACENT MUCOUS MEMBRANES
Authors: Kaneko Fumio , Kawagishi Ikuro , Miura Yusho , Kobayashi Kunihiko 
Published: April 2015

ABSTRACTThe distribution of secretory IgA [sIgA] components (IgA, secretory component [SC] and J‐chain) was investigated in normal skin, pathological skin, and the mucous membrane immunofluorescently.In three of fifteen normal skin samples, very weak deposits of sIgA‐components were detected at the upper portion of the epidermis and SC was found weakly at the dermo‐epidermal (D‐E) junction. Normal mucous membrane from the lip, the oral cavity, and the glans penis showed the presence of sIgA components in the upper, vacuolated epithelial cells. In one of three specimens from the oral cavity, deposition of SC was found weakly at the proprial‐epithelial (P‐E) junction. IgA‐bearing cells were detected in the proprial layer. In the pathological skin showing epidermal involvement, marked visible deposits of SC were found at the D‐E junction and also around dilated vessels in the dermal papillae. In aphthous lesions of the oral cavity, SC was located at the P‐E junction. SIgA components were detectable in the stratum corneum of these skin diseases and on the surface of involved mucosae.These findings may suggest that sIgA is concerned with the surface immunological defence system of the skin and mucous membranes.
IMMUNOHISTOCHEMICAL DEMONSTRATION OF POLY(ADENOSINE DIPHOSPHATE‐RIBOSE) SYNTHESIS IN HUMAN SKIN
Authors: Ikai Kouichi , Danno Kiichiro , Imamura Sadao , Ueda Kunihiro 
Published: April 2015

ABSTRACTThe distribution of poly(adenosine diphosphate‐ribose) [poly(ADP‐ribose)] synthesis in human skin was investigated by an indirect immunofluorescence technique using a specific antibody against poly(ADP‐ribose). In normal skin, the specific immunofluorescence of poly(ADP‐ribose) was observed in nuclei of not only epidermal cells but also cells in the dermis, including the hair follicle and sweat gland. Preincubation of tissue sections with NAD, a substrate for poly(ADP‐ribose) synthesis, intensified the immunofluorescence of poly(ADP‐ribose). Nuclear immunofluorescence was prominent in the lower and middle layers of the epidermis and became weaker in the upper layers in parallel with the disappearance of the nuclei, especially in tissues untreated with NAD.The immunostaining pattern of poly(ADP‐ribose) synthesis in psoriatic epidermis was similar to that of normal skin except that fluorescence was observed in nuclei of the parakeratotic horny layer.In affected cancerous skin, immunofluorescence of poly(ADP‐ribose) was weak in nuclei of cells in the inner layers of horn pearl of well differentiated squamous cell carcinoma, although fluorescence was prominent in the outer layer cells of horn pearl. In contrast, tumor cells of both malignant melanoma and basal cell epithelioma exhibited the nuclear immunofluorescence of poly(ADP‐ribose) synthesis. These results suggest that the capacity for synthesizing poly(ADP‐ribose) may be related to the differentiation of epidermal cells.
SENSITIZATION POTENCY OF SOME PHENOLIC COMPOUNDS
Authors: Itoh Masatoshi 
Published: April 2015

AbstractSkin irritability and sensitization potency of 14 phenolic compounds were studied on guinea pigs. When 10% preparations of each compound were used for induction, and 1% preparations were used for the challenge, the sensitization frequencies were 80% for isoeugenol and isosafro eugenol, 67% for isohomogenol, 40% for acetyl isoeugenol, 20% for eugenol, and 5% for dihydro eugenol. No animals were sensitized by methyl eugenol, acetyl eugenol, methyl isoeugenol or benzyl isoeugenol. The sensitization frequency was 80% for catechol, 89% for guaethol, 14% for p‐n‐propyl phenol, and 0% for phenol.The frequency of positive allergic reactions to patch tests on patients with cosmetic dermatitis was 2.6% for eugenol and 5.2% for isoeugenol. The frequency of cross reactions on patients sensitive to isoeugenol was also studied.The relationship between chemical structures and sensitization potencies as well as other factors influencing the results of patch tests on patients are discussed.
CELL KINETIC STUDIES OF THE EFFECT OF VARIOUS KINDS OF CHEMOTHERAPEUTIC AGENTS ON A HUMAN MELANOMA CELL LINE (SEKI, II)
Authors: Shimada Shinji , Kawashima Makoto , Watanabe Shinichi , Yamada Kiyoshi , Mizoguchi Masako , Hori Yoshiaki , Kukita Atsushi 
Published: April 2015

AbstractThe cell cycle phase distribution of a human malignant melanoma cell line (SEKI, II) exposed to various chemotherapeutic agents was investigated by flow‐cytometry. The accumulation effect on the radiosensitive G2 and M phases was nearly 100% in the vincristine 0.1 μg/ml group, 75.2% in the ACNU 5 μg/ml group, and 53.1 % in the pepleomycin 1 μg/ml group, respectively. The groups treated with adriamycin (1 μg/ml and 10 μg/ml) or prednisolone (10 μg/ml) were not significantly different from the control group. It is suggested that vincristine, ACNU and pepleomycin may be useful for synchronization radiation therapy.
ULTRASTRUCTURE OF AN ATROPHIC TYPE OF SENILE KERATOSIS
Authors: Kimura Shunji 
Published: April 2015

AbstractThree cases of an atrophic type of senile keratosis were examined by electron microscopy. All three cases showed similar changes, although some variability from case to case was observed. Lesional epidermis showed remarkable widening of the intercellular spaces with a decrease in the number of desmosomes and detachment of desmosomal connections. Formation of intracytoplasmic vacuoles was also prominent. These changes were localized mainly in the lower part of the epidermis, while the upper epidermis was relatively intact. Discontinuity and multiplication of the epidermal basal lamina were sometimes observed. From these findings, it is concluded that this atrophic type of senile keratosis is characterized by ‘déhiscence cellulaire' with minimal dyskeratosis, although it can properly be regarded as an epidermal precancerous condition.
BIOCHEMICAL ANALYSIS OF HAIR GROWTH FROM THE ASPECTS OF AGING AND ENZYME ACTIVITIES
Authors: Hattori Michihiro , Ogawa Hideoki 
Published: April 2015

ABSTRACTThe dorsal resting hair of C3H mice was shaved, activating the hair into the anagen stage. Hair regrowth in mice of different ages (10–20 mice in each group), whose hair cycles were in the telogen stage, were compared after shaving. In both groups of mice, new hair growth was not uniform, but irregular on the shaved areas. Delay in hair regrowth increased as the mice became older (20 < 66 < 188 < 312 day old mice). Furthermore, eighteen 59 day‐old mice were used for biochemical analysis. Nine days after shaving, 30–50% of the shaved skin area was covered with new hair. After sacrifice, the dorsal skins were separated into hair re‐growing, non‐growing, and non‐shaved control areas. Several enzyme activities in the extracts from the above‐named areas were compared in terms of “per g of wet weight tissue”, “per mg DNA” and “per mg of soluble protein”. The activities of ornithine decarboxylase (ODC), transglutaminase (TGase) and alkaline phosphatase (Al‐P) were remarkably high in the extract from the hair re‐growing area as compared with that from the non‐growing area. Comparison of the activities of the follicle‐rich fractions from the hair re‐growing area to those of the non‐shaved area showed that ODC, TGase and Al‐P activities were increased.
ACQUIRED FIBROKERATOMA
Authors: Kikuchi Ichiro , Taketomi Isao 
Published: April 2015

ABSTRACTAn acquired fibrokeratoma which had developed on the sole of the right foot of a woman over the past 37 years was removed surgically at the age of 55. It was shaped like a fleshy leaf. Histologically, it was characterized by a protrusion of the sole devoid of skin appendages.
IN VITRO STUDY OF CHLORPROMAZINE PHOTOSENSITIZATION: PHOTOINACTIVATION OF BACTERIOPHAGE RNA IN THE PRESENCE OF CHLORPROMAZINE
Authors: Matsuo Itsuro , Ohkido Muneo , Fujita Hitoshi , Kawano Katsumi , Suzuki Kenshi 
Published: April 2015

ABSTRACTEffects of chlorpromazine plus UVA‐illumination on RNA were studied in vitro. Chlorpromazine‐UVA treatment of RNA extracted from bacteriophages MS2 and Qβ reduced their infectivity to Escherichia coli spheroplasts. Extent of the inactivation was increased with increasing concentrations of chlorpromazine and with increasing fluence of UVA. In the light of our previous findings, chlorpromazine‐adduct to purine residues is suggested as a possible source of damage leading to the inactivation of RNA.
CUTANEOUS OXYGEN TENSION MEASUREMENTS IN PATIENTS WITH VARIOUS PERIPHERAL CIRCULATORY DISTURBANCES OF THE SKIN
Authors: Nishioka Kazue , Asagami Chidori , Okazaki Yasunori , Hisamoto Kazuo , Uchiday Hiroshi 
Published: April 2015

ABSTRACTCutaneous oxygen tension (cutaneous pO2) was measured in 59 healthy controls and 58 patients with peripheral circulatory disturbances including 18 with progressive systemic sclerosis, 5 with stasis dermatitis with ulcer, 22 with arteriosclerosis obliterans, and 13 with thromboangiitis obliterans.In the control group, the mean value ± SD of cutaneous pO2 was 82 ± 10 mmHg on the forearm, 79 ± 10 mmHg on the subclavicular region, 81 + 11 mmHg on the leg, 75 ± 11 mmHg on the dorsum of the hand and 70 ± 9 mmHg on the dorsum of the foot. There was an inverse correlation between cutaneous pO2 values on the forearm and increase in age, and a significant correlation between cutaneous pO2 values on the forearm and femoral arterial oxygen tension (PaO2).In all of the patient groups, cutaneous pO2 measured at affected sites was significantly lower than in the control group.These cutaneous pO2 values reflect the amount of O2 available for diffusion to the skin surface; thus cutaneous pO2 measurement is useful as a quantitative evaluation of cutaneous circulation.
SDS‐PAGE ANALYSIS OF WHOLE AND FRACTIONATED PSORIATIC EPIDERMIS
Authors: Kato Naoko , Umeda Katsuko , Iizuka Hajime , Aoyagi Takashi , Miura Yusho 
Published: April 2015

ABSTRACTThe SDS‐PAGE patterns of keratinous proteins extracted from whole epidermis and fractionated epidermal cells were studied. Those from the whole and fractionated epidermal cells of psoriatic involved skin showed an extra 58,000 dalton polypeptide in addition to the four major polypeptides (M.W.: 67,000, 63,000, 59,000 and 55,000) which were demonstrated to be common to normal epidermis and psoriatic‐uninvolved epidermis. The stratum corneum of psoriatic involved skin did not contain a 67,000 dalton polypeptide which was present in the stratum corneum of normal and psoriatic uninvolved skin.The presence of the additional 58,000 dalton polypeptide may be due to abnormal synthesis of keratin proteins in the psoriatic epidermis, since this additional polypeptide was present in the lower living layers of the psoriatic epidermis as well. On the other hand, the absence of the 67,000 dalton polypeptide in the psoriatic stratum corneum may be due to enzymatic modifications during the transition from the living cell layers to the horny layer.
THE EFFECTS OF POTENT TOPICAL CORTICOSTEROIDS ON ADRENOCORTICAL FUNCTION
Authors: Aso Miki 
Published: April 2015

ABSTRACTForty‐eight patients were treated using nine potent corticosteroid ointments administered by simple application and their adrenocortical function was examined during treatment. Adrenocortical function was not suppressed by beclomethasone dipropionate, betamethasone valerate, or fluocinonide; was moderately suppressed by diflucortolone valerate, budesonide, and halcinonide; and was markedly suppressed by clobetasol propionate, desoximetasone, and diflorasone diacetate.The dose relationship between topically applied clobetasol propionate ointment and orally administered betamethasone on adrenocortical suppression was also studied. The results suggest that excessive application of very strong topical corticosteroids has much the same effects as systemic administration of small doses of corticosteroids.
COEXISTENCE OF LINEAR IGA DERMATITIS HERPETIFORMIS AND SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: Thaipisuttikul Yupin , Piamphongsant Thada , Suwanwela Nibha 
Published: April 2015

ABSTRACTA vesiculobullous eruption of dermatitis herpetiformis and systemic lupus erythematosus developing simultaneously in a 26 year old woman is described. The lesions were characterized by pruritic urticarial vesicles, eosinophilic and neutrophilic abscesses, and the presence of the linear type of IgA deposition at the basement membrane zone. These lesions respond to dapsone, although high dosages of prednisone and cyclophosphamide were not effective.
BEHÇET'S DISEASE LEUCOCYTE INHIBITORY FACTOR (LIF) IN RESPONSE TO SKIN EXTRACT
Authors: GILHAR A. , HAIM S. , WOLF V. , GOLAN D. 
Published: April 2015

ABSTRACTThis is a study of leucocyte inhibitory factor (LIF) in patients with Behçet's disease using an extract from a reactive (pathergic) lesion as an antigen and, as a control, an extract from normal skin of a normal subject. The purpose is to search for the possible existence of an antigen in the skin that may explain the development of this reactive lesion following a trivial trauma.The LIF was tested in 9 patients with the disease; in 7 both during remission and exacerbation of symptoms, and in 9 healthy matched controls. The results failed to indicate any existence of an antigen in the skin of the tested patients.
NAIL CANDIDIASIS
Authors: Watanabe Shin'Ichi , Seki Yoshihito , Shimozuma Michiro , Takizawa Kiyohiro 
Published: April 2015

ABSTRACTOnychomycosis caused by Candida is often accompanied by paronychia. Not only the morbid changes in the nailplate caused by the true parasitism by Candida but also the secondary changes in the nail due to candidial paronychia are sometimes handled as though they were candidial onychia.Onychomycosis caused by candidial infection, i.e., nail candidiasis, is entirely different in its pathogenetic respects from the secondary ungual changes due to candidial paronychia. The reason why both are classified together as candidial onychia appears attributable to the ambiguous characterization of their clinical and pathological features.We therefore undertook a statistical analysis of 765 cases of candidiasis who visited the Dermatological Department of Tokyo University Hospital during the period from 1969 to 1980. Of these, 25 cases of nail candidiasis (primary onychia) were sorted out, followed by clinical and pathological examinations.The results showed that nail candidiasis appeared in 14.3% of the candidial paronychia and onychia cases, and no significant differences from candidial paronychia were attributable to age or sex. On the other hand, it was found that nail candidiasis was clinically characterized by subungual hyperkeratosis, while the fungal element appeared histopathologically in the lower part of the nailplate, taking an abnormal parasitic form similar to that in dermatophytes.The pathogen causing nail candidiasis was Candida albicans and was resistant to various therapies. The only effective therapy was oral administration of Ketoconazole.Nail candidiasis is often accompanied by underlying diseases like SLE which result in cellular immunodeficiency. While chronic mucocutaneous candidiasis occurs with a background of congenital cellular immunodeficiency to Candida, nail candidiasis appears to have an acquired immunodeficiency as the background.In short, nail candidiasis can be interpreted as a candidiasis which occurs when the host/parasite relations are disturbed. In this sense, it appears that nail candidiasis can be regarded as a dermadrome which reflects cellular immunodeficiency of host.
INTRALESIONAL HUMAN FIBROBLAST INTERFERON IN COMMON WARTS
Authors: Niimura Michihito 
Published: April 2015

ABSTRACTIn a double‐blind trial, patients with bilateral common warts of the extremities were treated at weekly intervals with intralesional injections of either human fibroblast interferon or placebo. More than 81% of the interferon‐treated extremities were either cured or responded effectively to therapy. Only 17% of the placebo‐treated lesions responded in this fashion. A statistical analysis of these data confirms the effectiveness of intralesional interferon therapy. No adverse effects were observed.