bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with disability and impact of tension-type headache: findings of the Korean headache survey
Authors: Kim Byung-Su , Chung Chin-Sang , Chu Min Kyung , Chung Yun Kyung , Lee Chung-Bin , Kim Jae-Moon 
Published: May 2015

Abstract

Background
Although mostly mild in symptom severity, tension-type headache (TTH) can cause disability. However, factors associated with disability of TTH have been rarely reported. This study sought to assess the factors associated with TTH-related disability and impact.

Methods
We analyzed data form the Korean Headache Survey, a nation-wide survey regarding headache in all Korean adults aged 19–69 years. TTH-related disability was measured by surveying actual disability and Headache Impact Test-6 (HIT-6). Actual disability was defined as having one or more days of activity restriction or missed activity due to headache in the last 3 months. The HIT-6 score ≥ 50 was regarded as significant headache impact associated with TTH. We assessed factors associated with TTH-related disability and impact using logistic regression analyses adjusting for sociodemographic variables and headache characteristics.

Results
Among 1507 individuals, the 1-year prevalence rate of TTH was 30.7% (n = 463), of which 4.8% reported actual disability and 21.3% had headache impact, respectively. In univariate analyses, sociodemographic variables were not associated with actual disability and headache impact, respectively. There were relationships between several headache characteristics and actual disability/headache impact. After adjustment of potential confounders, moderate headache intensity was correlated with actual disability (odds ratio [OR]: 4.41, 95% confidence interval [CI]: 1.46–13.27), while an inverse association was observed between no aggravation by routine activity and actual disability (OR: 0.32, 95% CI: 0.12–0.88). Multivariate analyses showed that ORs for headache impact were increased in those with higher headache frequency (OR: 2.54, 95% CI: 1.47–4.39 for 1–14 days/month; OR: 23.83, 95% CI: 5.46–104.03 for ≥ 15 days/month), longer headache time duration (OR: 1.84, 95% CI: 1.04–3.25 for ≥ 1 and < 4 hours; OR: 2.44 95% CI: 1.17–5.11 for ≥ 4 hours), and phonophobia (OR: 1.73, 95% CI: 1.02–2.95), whereas decreased in those with no aggravation by routine activity (OR: 0.32, 95% CI: 0.12–0.88).

Conclusions
Several headache characteristics were associated with actual disability and headache impact among TTH individuals. Our findings suggest that there needs to be consideration careful of troublesome headache characteristics for TTH individuals suffering from disability and impact.
Short hairpin RNA- mediated gene knockdown of FOXM1 inhibits the proliferation and metastasis of human colon cancer cells through reversal of epithelial-to-mesenchymal transformation
Authors: Yang KanKan , Jiang LinHua , Hu You , Yu Jing , Chen HenFeng , Yao YiZhou , Zhu XinGuo 
Published: May 2015

Abstract
Background
The Forkhead box M1 (FOXM1) is an oncogenic transcription factor and plays a significant role in cell EMT, proliferation, metastasis in a multitude of human solid tumors including colorectal cancer (CRC). However, the underlying molecular mechanisms by which FoxM1 contributes to epithelial-to-mesenchymal (EMT) and metastasis have not been fully elucidated in CRC.

Methods
In our study, we investigated FOXM1 protein expression in 87 CRC tissue specimens, invasive lymph nodes and adjacent paired normal colorectal tissues by immunohistochemical analysis. Then we transfected FOXM1 specific shRNA into SW620 cells to examine effect of FOXM1 on proliferation, colony formation, migration and invasion in vitro. Western blotting and real-time PCR were used to detect the protein and mRNA expression of FOXM1 and EMT-related markers.

Results
FOXM1 was overexpressed in CRC tissues, invasive lymph nodes and CRC cell lines. FoxM1 overexpression was significantly associated with lymph node metastasis (P < 0.001), and tumor recurrence (P < 0.001). Moreover, downregulation of FOXM1 in SW620 cells by shRNA approach inhibited cell growth, clonogenicity, migration and invasion in vitro. In addition, decreased FOXM1 expression in SW620 cells reversed the acquisition of EMT phenotype by up-regulating E-cadherin, as well as reduction Vimentin and Snail expressions at protein and mRNA levels.

Conclusions
FOXM1 may regulate CRC cells metastasis through EMT program and FOXM1 may be a potential target for treatment of CRC.
Metformin increases survival in hormone receptor-positive, HER2-positive breast cancer patients with diabetes
Authors: Kim Hee Jeong , Kwon Hyunwook , Lee Jong Won , Kim Hwa Jung , Lee Sae Byul , Park Hee Sung , Sohn Guiyun , Lee Yura , Koh Beom Seok , Yu Jong Han , Son Byung Ho , Ahn Sei Hyun 
Published: May 2015

Abstract
Introduction
Metformin use has recently been observed to decrease both the rate and mortality of breast cancer. Our study was aim to determine whether metformin use is associated with survival in diabetic breast cancer patients by breast cancer subtype and systemic treatment.

Methods
Data from the Asan Medical Center Breast Cancer Database from 1997 to 2007 were analyzed. The study cohort comprised 6,967 nondiabetic patients, 202 diabetic patients treated with metformin, and 184 diabetic patients that did not receive metformin. Patients who were divided into three groups by diabetes status and metformin use were also divided into four subgroups by hormone receptor and HER2-neu status.

Results
In Kaplan-Meier analysis, the metformin group had a significantly better overall and cancer specific survival outcome compared with non metformin diabetic group (P <0.005 for both). There was no difference in survival between the nondiabetic and metformin groups. In multivariate analysis, Compared with metformin group, patients who did not receive metformin tended to have a higher risk of metastasis with HR 5.37 (95 % CI, 1.88 to 15.28) and breast cancer death with HR 6.51 (95 % CI, 1.88 to 15.28) on the hormone receptor-positive and HER2-negative breast cancer. The significant survival benefit of metformin observed in diabetic patients who received chemotherapy and endocrine therapy (HR for disease free survival 2.14; 95 % CI 1.14 to 4.04) was not seen in diabetic patients who did not receive these treatments.

Conclusion
Patients receiving metformin treatment when breast cancer diagnosis show a better prognosis only if they have hormone receptor-positive, HER2-positive tumors. Metformin treatment might provide a survival benefit when added to systemic therapy in diabetic patients.
Novel APC promoter and exon 1B deletion and allelic silencing in three mutation-negative classic familial adenomatous polyposis families
Authors: Lin Yiing , Lin Shin , Baxter Melanie D , Lin Lawrence , Kennedy Susan M , Zhang Zhengyan , Goodfellow Paul J , Chapman William C , Davidson Nicholas O 
Published: May 2015

Abstract
Background
The overwhelming majority (approximately 80%) of individuals with classic familial adenomatous polyposis (FAP) exhibit mutations in the coding sequence of the adenomatous polyposis coli (APC) tumor suppressor gene. Families without detectable APC mutations are unable to benefit from the use of genetic testing for clinical management of this autosomal dominant syndrome.

Methods
We used exome sequencing and linkage analysis, coupled with second-generation sequencing of the APC locus including non-coding regions to investigate three APC mutation-negative classical FAP families.

Results
We identified a novel ~11 kb deletion localized 44 kb upstream of the transcription start site of APC that encompasses the APC 1B promoter and exon. This deletion was present only in affected family members of one kindred with classical FAP. Furthermore, this same deletion with identical breakpoints was found in the probands of two additional APC mutation-negative classical FAP kindreds. Phasing analysis of single nucleotide polymorphisms (SNPs) around the deletion site in the three probands showed evidence of a shared haplotype, suggesting a common founder deletion in the three kindreds. SNP analysis within the coding sequence of APC, revealed that this ~11 kb deletion was accompanied by silencing of one of the APC alleles in blood-derived RNA of affected individuals.

Conclusions
These results support the causal role of a novel promoter deletion in FAP and suggest that non-coding deletions, identifiable using second-generation sequencing methods, may account for a significant fraction of APC mutation-negative classical FAP families.
JAFFA: High sensitivity transcriptome-focused fusion gene detection
Authors: Davidson Nadia M , Majewski Ian J , Oshlack Alicia 
Published: May 2015

AbstractGenomic instability is a hallmark of cancer and, as such, structural alterations and fusion genes are common events in the cancer landscape. RNA sequencing (RNA-Seq) is a powerful method for profiling cancers, but current methods for identifying fusion genes are optimised for short reads. JAFFA (https://github.com/Oshlack/JAFFA/wiki) is a sensitive fusion detection method that outperforms other methods with reads of 100 bp or greater. JAFFA compares a cancer transcriptome to the reference transcriptome, rather than the genome, where the cancer transcriptome is inferred using long reads directly or by de novo assembling short reads.
A study of development of transmission systems for next-generation terrestrial 4 K UHD and HD convergence broadcasting
Authors: Oh Jong Gyu , Won Yong Ju , Lee Jin Seop , Kim Yong-Hwan , Paik Jong Ho , Kim Joon Tae 
Published: May 2015

Abstract
The worldwide transition from analog to digital broadcasting has now been completed, and the need to study next-generation standards for ultra-high-definition TV (UHDTV) broadcasting, as well as broadcasting and communication convergence systems is rapidly growing. In particular, high-resolution mobile broadcasting services are needed to satisfy recent consumer demands. Therefore, the development of highly efficient convergence broadcasting systems that provide fixed/mobile broadcasting through a single channel is needed. In this paper, a service scenario and the requirements for providing 4 K UHD and high-definition (HD) convergence broadcasting services through a terrestrial single channel are analyzed by employing the latest transmission and video codec technologies. Optimized transmission parameters for 6- and 8-MHz terrestrial bandwidths are drawn, and receiving performances are measured under additive white Gaussian noise (AWGN) and time-varying typical urban (TU)-6 channel to find the threshold of visibility (TOV). From the results, reliable receiving of HD layer data can be achieved at a 6-MHz bandwidth when the maximum receiver velocity is 140 km/h and no higher due to the limit of bandwidth. When the bandwidth is extended to 8 MHz, reliable receiving of both 4 K UHD and HD layer data can be achieved under a very fast fading multipath channel.
CCR2 deficiency leads to increased eosinophils, alternative macrophage activation, and type 2 cytokine expression in adipose tissue
Authors: Bolus W Reid , Gutierrez Dario A , Kennedy Arion J , Anderson-Baucum Emily K , Hasty Alyssa H 
Published: May 2015

Abstract
Adipose tissue (AT) inflammation during obesity is mediated by immune cells and closely correlates with systemic insulin resistance. In lean AT, eosinophils are present in low but significant numbers and capable of promoting alternative macrophage activation in an IL-4/IL-13-dependent manner. In WT mice, obesity causes the proportion of AT eosinophils to decline, concomitant with inflammation and classical activation of AT macrophages. In this study, we show that CCR2 deficiency leads to increased eosinophil accumulation in AT. Furthermore, in contrast to WT mice, the increase in eosinophils in CCR2−/− AT is sustained and even amplified during obesity. Interestingly, a significant portion of eosinophils is found in CLSs in AT of obese CCR2−/− mice, which is the first time eosinophils have been shown to localize to these inflammatory hot spots. CCR2−/− bone marrow precursors displayed increased expression of various key eosinophil genes during in vitro differentiation to eosinophils, suggesting a potentially altered eosinophil phenotype in the absence of CCR2. In addition, the proportion of eosinophils in AT positively correlated with local expression of Il5, a potent eosinophil stimulator. The increase in eosinophils in CCR2−/− mice was detected in all white fat pads analyzed and in the peritoneal cavity but not in bone marrow, blood, spleen, or liver. In AT of CCR2−/− mice, an increased eosinophil number positively correlated with M2-like macrophages, expression of the Treg marker Foxp3, and type 2 cytokines, Il4, Il5, and Il13. This is the first study to link CCR2 function with regulation of AT eosinophil accumulation.
Control of Wnt5b secretion by Wntless modulates chondrogenic cell proliferation through fine-tuning fgf3 expression
Authors: Wu Bo-Tsung , Wen Shih-Hsien , Hwang Sheng-Ping L. , Huang Chang-Jen , Kuan Yung-Shu 
Published: May 2015

ABSTRACT
Wnts and Fgfs regulate various tissues development in vertebrates. However, how regional Wnt or Fgf activities are established and how they interact in any given developmental event is elusive. Here, we investigated the Wnt-mediated craniofacial cartilage development in zebrafish and found that fgf3 expression in the pharyngeal pouches is differentially reduced along the anteroposterior axis in wnt5b mutants and wntless (wls) morphants, but its expression is normal in wnt9a and wnt11 morphants. Introducing fgf3 mRNAs rescued the cartilage defects in Wnt5b- and Wls-deficient larvae. In wls morphants, endogenous Wls expression is not detectable but maternally deposited Wls is present in eggs, which might account for the lack of axis defects in wls morphants. Secretion of endogenous Wnt5b but not Wnt11 was affected in the pharyngeal tissue of Wls morphants, indicating that Wls is not involved in every Wnt secretion event. Furthermore, cell proliferation but not apoptosis in the developing jaw was affected in Wnt5b- and Wls-deficient embryos. Therefore, Wnt5b requires Wls for its secretion and regulates the proliferation of chondrogenic cells through fine-tuning the expression of fgf3 during jaw cartilage development.
SEA you later alli-GATOR – a dynamic regulator of the TORC1 stress response pathway
Authors: Dokudovskaya Svetlana , Rout Michael P. 
Published: May 2015

ABSTRACT
Cells constantly adapt to various environmental changes and stresses. The way in which nutrient and stress levels in a cell feed back to control metabolism and growth are, unsurprisingly, extremely complex, as responding with great sensitivity and speed to the ‘feast or famine, slack or stress’ status of its environment is a central goal for any organism. The highly conserved target of rapamycin complex 1 (TORC1) controls eukaryotic cell growth and response to a variety of signals, including nutrients, hormones and stresses, and plays the key role in the regulation of autophagy. A lot of attention has been paid recently to the factors in this pathway functioning upstream of TORC1. In this Commentary, we focus on a major, newly discovered upstream regulator of TORC1 – the multiprotein SEA complex, also known as GATOR. We describe the structural and functional features of the yeast complex and its mammalian homolog, and their involvement in the regulation of the TORC1 pathway and TORC1-independent processes. We will also provide an overview of the consequences of GATOR deregulation in cancer and other diseases.
Science and the public: The public understanding of science and its measurements
Authors: Entradas Marta 
Published: May 2015

Abstract
This is the first of a two-part historical review on the relationship between science and its publics in the second half of the twentieth century. The two-part literature review covers major trends in the evolution of this relationship from ‘public understanding of science’ (PUS) in which science was separated from laypeople, through the transition to ‘public participation’ when PUS became a matter for science policy. This first part of the literature describes the arguments that called for an increase in the public’s understanding of science, its measurement, and the academic debate in favour of and against PUS measures. In particular, we refer to the evolution of survey design, and how criticisms of measures of PUS gave rise to the ‘contextual’ perspective in the PUS being favoured over a ‘deficit’ one.
Canine Adipose-Derived Stem Cell Aggregates as a Viable Substitute to Actual Canine Dermal Papillae
Authors: Bae Sohee , Kim Jina , Li Li , Lee Aeri , Lim Hyunjoo , Jeong Junemoe , Lee Seung Hoon , Kweon Oh-kyeong , Kim Wan Hee 
Published: May 2015

Abstract
Hair loss is a major dermatological disease in veterinary and human medicine. Active studies on hair regeneration with mesenchymal stem cells have been performed in an effort to solve the limitations of conservative treatments in human medicine. Our understanding of the canine hair follicle (HF), considering a useful model for the study of the human alopecia, is limited. This study was designed to broaden our understanding of canine dermal papilla (DP), and to reconstruct dermal papilla-like tissue (DPLT) using canine adipose-derived mesenchymal stem cells (AD-MSCs), as an alternative to actual DP. We cultured canine DPs, observed their culture patterns and compared their expression level of DP-related genes and proteins with those of DPLTs by performing RT-PCR analysis and Western blotting. Canine dermal papilla cells (DPCs) showed multilayer culture patterns with pseudo-papillae. Reconstruction of DPLTs was performed successfully. Not only were they morphologically similar to actual DPs, but we also observed similarities between DPCs and DPLTs in molecular characteristics. These findings suggested that DPLT was a viable substitute for DP. This study will not only be helpful for understanding the morphological and molecular characteristics of canine DPCs, but may also serve as a basis for understanding human hair follicle biology and potential therapeutic strategies for alopecia.
Geotechnical Risk Classification for Underground Mines / Klasyfikacja Poziomu Zagrożenia Geotechnicznego W Kopalniach Podziemnych
Authors: Mishra Ritesh Kumar , Rinne Mikael 
Published: May 2015

Abstract
Underground mining activities are prone to major hazards largely owing to geotechnical reasons. Mining combined with the confined working space and uncertain geotechnical data leads to hazards having the potential of catastrophic consequences. These incidents have the potential of causing multiple fatalities and large financial damages. Use of formal risk assessment in the past has demonstrated an important role in the prediction and prevention of accidents in risk prone industries such as petroleum, nuclear and aviation. This paper proposes a classification system for underground mining operations based on their geotechnical risk levels. The classification is done based on the type of mining method employed and the rock mass in which it is carried out. Mining methods have been classified in groups which offer similar geotechnical risk. The rock mass classification has been proposed based on bulk rock mass properties which are collected as part of the routine mine planning. This classification has been subdivided for various stages of mine planning to suit the extent of available data. Alpha-numeric coding has been proposed to identify a mining operation based on the competency of rock and risk of geotechnical failures. This alpha numeric coding has been further extended to identify mining activity under ‘Geotechnical Hazard Potential (GHP)’. GHP has been proposed to be used as a preliminary tool of risk assessment and risk ranking for a mining activity. The aim of such classification is to be used as a guideline for the justification of a formal geotechnical risk assessment.
Method for Virtual Prototyping of Cabins of Mining Machines Operators / Metoda Wirtualnego Prototypowania Kabin Operatorów Maszyn Górniczych
Authors: Tokarczyk Jarosław 
Published: May 2015

Abstract
Method for virtual prototyping of cabins of mining machines operators is presented in the light of anthropotechnical assessment criteria. Anthropotechnical criteria and design of models of anthropometric features, which are used for assessment of design solutions in the aspect of safety criterion, are divided and discussed. Developed virtual prototyping method for assessment of cabin of underground locomotive operator was used. Initial simulation was made with use of Finite Element Method.
Sedimentary Rocks Associated with the Coal Seams of the Saddle Beds from the Chwałowice Trough – West Part of Upper Silesian Coal Basin / Skały Osadowe Towarzyszące Pokładom Węgla Warstw Siodłowych Niecki Chwałowickiej – Zachodnia Część Górnośląskiego Zagłębia Węglowego
Authors: Stanienda Katarzyna 
Published: May 2015

Abstract
The results of investigation of the associated rocks with the Saddle Beds Coals Seams from Chwałowice Trough were presented in this article. The results of researches show that sandstones and mudstones dominate in the investigating profile. Claystones are situated mainly in ceilings and floors of coal seams. Grains of sandstones include mainly quartz, feldspars, micas and also quartzite’s and gneiss’s fragments. It is also possible to observe small grains of heavy minerals. Some of sandstones present bad compactness and are destroyed during transportation. This feature is connected with presence of little quantity of cement, especially in medium grained sandstones, which include more matrix than typical cement. The cement is built mainly of clay minerals, kaolinite and illite, carbonates and chalcedony but X-ray diffraction confirmed also the presence of halite in the cement of investigated sandstones.
Mudstones and claystones are composed mainly of clay minerals. It’s also possible to find quartz and micas there. Higher amounts of quartz and micas are possible to find rather in mudstones.
The associated rocks with the Saddle Beds Coal Seams from Chwałowice Trough include also organic matter.
Bioleaching of hazardous waste
Authors: Drobíková Klára , Rozumová Lucia , Otoupalíková Hana , Seidlerová Jana 
Published: May 2015

AbstractLandfill represents the least environmentally-friendly method of waste disposal because of possible pollution to the environment. Dangerous wastes pose the greatest problems and are often disposed of by combustion. This process reduces their volume but entails the formation of new types of dangerous waste. The present study focuses on the possibilities of the removal of the hazardous properties of waste originating from hazardous waste incinerators (three types of bottom ash and charcoal from flue gas cleaning) by bioleaching. Toxic pollutants originating from waste could be removed by bioleaching with Acidithiobacillus ferrooxidans. The effectiveness of bioleaching was evaluated on the basis of the pollutant content in the aqueous leachates. For studying the relation between the efficiency of bioleaching and the binding of pollutants in the waste, Tessier’s sequential extraction was used. A comparison of bioleaching efficiency and the results of sequential extraction shows that bioleaching can be used to remove elements which are in an exchangeable form or are bound to carbonates, meaning that they are bound in bio-available forms. Bacterial activity was also shown to change the bonds of pollutants in wastes, leading to increased solubility of the pollutant.
Polymeric ionic liquids: a strategy for preparation of novel polymeric materials
Authors: Farzin Manoochehr , Nosratzadegan Keyvan , Azarnia Jamshid , Ferdosi Mohammad 
Published: May 2015

AbstractA novel polymeric ionic liquid (PIL), bearing high C-N and N-N content, potentially suitable for new safe energetic materials and catalyst supports was introduced. The PIL was prepared by way of radical co-polymerisation of 1-vinyl-3-p-nitrobenzylimidazolium bromide and 1-vinylimidazole at 80◦C using azobisisobutyronitrile (AIBN) as an initiator. The PIL thus produced was successfully transformed into NO