bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetric chromosome segregation in Xanthomonas citri ssp. citri
Authors: Ucci Amanda P. , Martins Paula M. M. , Lau Ivy F. , Bacci Maurício , Belasque José , Ferreira Henrique 
Published: December 2013

AbstractThis study was intended to characterize the chromosome segregation process of Xanthomonas citri ssp. citri (Xac) by investigating the functionality of the ParB factor encoded on its chromosome, and its requirement for cell viability and virulence. Using TAP tagging we show that ParB is expressed in Xac. Disruption of parB increased the cell doubling time and precluded the ability of Xac to colonize the host citrus. Moreover, Xac mutant cells expressing only truncated forms of ParB exhibited the classical phenotype of aberrant chromosome organization, and seemed affected in cell division judged by their reduced growth rate and the propensity to form filaments. The ParB‐GFP localization pattern in Xac was suggestive of an asymmetric mode of replicon partitioning, which together with the filamentation phenotype support the idea that Xac may control septum placement using mechanisms probably analogous to Caulobacter crescentus, and perhaps Vibrio cholerae, and Corynebacterium glutamicum. Xac exhibits asymmetric chromosome segregation, and the perturbation of this process leads to an inability to colonize the host plant.
Grape seed proanthocyanidin extract improves the hepatic glutathione metabolism in obese Zucker rats
Authors: Fernández‐Iglesias Anabel , Pajuelo David , Quesada Helena , Díaz Sabina , Bladé Cinta , Arola Lluís , Salvadó Maria Josepa , Mulero Miquel 
Published: December 2013

ScopeIncreased oxidative stress may play an important role in metabolic syndrome and related manifestations, including obesity, atherosclerosis, hypertension, and insulin resistance. Its relation to obesity is due to increased reactive oxygen species and/or decreased glutathione (GSH) antioxidant metabolism. Consequently, the activation of glutathione metabolism appears to be a central defense response to prevent oxidative stress. In this sense, dietary supplements with natural antioxidant molecules, including proanthocyanidins, may present a useful strategy of controlling and reducing complications of obesity, including hepatic steatosis.Materials and resultsWe assessed the grape seed proanthocyanidin extract (GSPE) effect on oxidative alterations related to genetically obese rats (Zucker rats) and, more specifically, to hepatic GSH metabolism. We demonstrate that the administration of GSPE reduced the oxidized glutathione accumulation increasing the total GSH/oxidized glutathione hepatic ratio and consequently decreasing the activation of antioxidant enzymes, including glutathione peroxidase, glutathione reductase, and glutathione S‐transferase, and increasing the total antioxidant capacity of the cell.ConclusionIn Zucker rats, the obesity‐induced oxidative stress related to liver glutathione alteration was mitigated by GSPE administration.
Cerebral injury in monochorionic twins with selective intrauterine growth restriction: a systematic review
Authors: Inklaar M. J. , van Klink J. M. M. , Stolk T. T. , van Zwet E. W. , Oepkes D. , Lopriore E. 
Published: December 2013

ABSTRACTObjectiveTo estimate incidence and risk factors of severe cerebral injury in survivors from monochorionic pregnancies with selective intrauterine growth restriction (sIUGR) and/or birth weight discordance (BWD).MethodsElectronic databases were searched for studies describing perinatal and neurologic outcome in monochorionic twins with sIUGR and/or BWD. Exclusion criteria were twin–twin transfusion syndrome, twin anemia–polycythemia sequence, selective feticide or laser treatment.ResultsEleven articles were included in the systematic review. Analysis was hampered by different methodology and definitions of cerebral injury. The incidence of severe cerebral injury varied from 0% to 33% (average 8%, 52/661), and was higher in studies including single intrauterine demise [odds ratio (OR) 2.92; 95% confidence interval (CI) 0.89‐9.56] and studies with a median gestational age at birth of ≤32 weeks (OR 1.56; 95% CI 1.06–2.27). The risk of severe cerebral injury was higher in pregnancies with abnormal umbilical artery Doppler (13.5% vs 2.5%; OR 7.69; 95% CI 2.56–25.00) and in larger twins (9% vs 5%; OR 1.93; 95% CI 0.95–3.92).ConclusionsThe incidence of severe cerebral injury in monochorionic twins with sIUGR and/or BWD is approximately 8% and is associated with abnormal umbilical artery Doppler, larger twins, intrauterine fetal demise and low gestational age at birth. © 2013 John Wiley & Sons, Ltd.
In vitro metabolism and metabolic effects of ajulemic acid, a synthetic cannabinoid agonist
Authors: Burstein Sumner H. , Tepper Mark A. 
Published: December 2013

AbstractAjulemic acid is a synthetic analog of ∆8‐THC‐11‐oic acid, the terminal metabolite of ∆8‐THC. Unlike ∆9‐THC, the psychoactive principle of Cannabis, it shows potent anti‐inflammatory action and has minimal CNS cannabimimetic activity. Its in vitro metabolism by hepatocytes from rats, dogs, cynomolgus monkeys and humans was studied and the results are reported here. Five metabolites, M1 to M5, were observed in human hepatocyte incubations. One metabolite, M5, a glucuronide, was observed in the chromatogram of canine hepatocyte incubations. In monkey hepatocyte incubations, M5 was observed in the chromatograms of both the 120 and 240 min samples, trace metabolite M1 (side‐chain hydroxyl) was observed in the 120 min samples, and trace metabolite M4 (side‐chain dehydrogenation) was observed in the 240 min samples. No metabolites were found in the rat hepatocyte incubations. Unchanged amounts of ajulemic acid detected after the 2‐h incubation were 103%, 90%, 86%, and 83% for rat, dog, monkey, and human hepatocytes, respectively. Additional studies were done to ascertain if ajulemic acid can inhibit the activities of five principal human cytochrome P450 isozymes; CYP1A2, CYP2C9, CYP2C19, CYP2D6, and CYP3A4/5. In contrast to the phytocannabinoids ∆9‐THC and CBD, no significant inhibition of cytochrome activity was observed. These data further support the conclusions reached in earlier reports on ajulemic acid's high margin of safety and suggest that it undergoes minimal metabolism and is not likely to interfere with the normal metabolism of drugs or endogenous substances.
Effect of growth stoichiometry on the structural properties of AlN films on thermally nitrided sapphire $(11\bar 20)$
Authors: Ueno Kohei , Kishikawa Eiji , Inoue Shigeru , Ohta Jitsuo , Fujioka Hiroshi , Oshima Masaharu , Fukuyama Hiroyuki 
Published: December 2013

AbstractHigh‐quality AlN epilayers were grown via pulsed sputtering deposition on thermally nitrided sapphire $(11\bar 20)$ with precise control of the N/Al ratio. Under slightly Al‐rich growth conditions, the growth of AlN epilayers on the thermally nitrided sapphire proceeded in a two‐dimensional mode from the initial stage of growth, and their surfaces were atomically flat stepped and terraced structures. The FWHM values of the X‐ray rocking curves were as low as 87 arcsec and 339 arcsec for the 0002 and $1 \bar 102$ diffractions, respectively, at a film thickness of 400 nm. The present approach is therefore quite promising for the low‐cost fabrication of AlGaN‐based UV optical devices. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A fixed point model for rate control and routing in cloud data center networks
Authors: Li Bo , Ma Xuefeng , Li Jianming , Zong Zhou 
Published: December 2013

ABSTRACTThis paper addresses the issues of rate control and routing for cloud data center networks. Based on the theory of the supply–demand equilibrium, we propose a fixed point model for formulating cloud network equilibrium problems in which the equilibrium conditions are given by nonlinear equations. We show that the network equilibrium point is the optimal solution of a nonlinear programming problem by utilizing the tools of the variational inequality and convex optimization. The augmented Lagrangian multiplier algorithm is used to solve the nonlinear programming problem for computing the network equilibrium point. Further consideration is given to the equilibrium problems of a cloud network with multirate multicast sessions. We evaluate our approach on some random networks with unicast and multicast sessions, and the results show the effectiveness of our approach in finding the optimal equilibrium rates. We further evaluate the performance of our approach through cloud data center network simulation under various parameter settings, and the results show that the performance of our algorithm can be tuned and improved by choosing appropriate parameter values. Copyright © 2013 John Wiley & Sons, Ltd.
Traceable attribute‐based signcryption
Authors: Wei Jianghong , Hu Xuexian , Liu Wenfen 
Published: December 2013

ABSTRACTSigncryption can provide confidentiality and authenticity for many cryptographic applications. In this study, we propose a new efficient attribute‐based signcryption scheme. This scheme achieves confidentiality against chosen ciphertext attacks and unforgeability against chosen messages attacks in the selective attribute model. In addition, our scheme enjoys traceability by use of non‐interactive witness indistinguishable proofs; that is, the authority can break the anonymity of users when necessary. Compared with previous works, our scheme has advantages in terms of functionality and efficiency simultaneously. Copyright © 2013 John Wiley & Sons, Ltd.
Likelihood‐based photograph identification: Application with photographs of free‐ranging bison
Authors: Merkle Jerod A. , Fortin Daniel 
Published: December 2013

ABSTRACTUsing photographs to identify individual animals and monitor populations is becoming more common. However, photographic identification methods where measurements of morphological traits (e.g., horn length) are compared have received little attention. We present an approach for aiding with the identification of individual animals from photographs. The approach incorporates measurement data, metadata from photographs, and multiple sources of error, and calculates a matching score between pairs of photographs using a likelihood‐based algorithm. We tested and identified the false‐rejection error rate using 91 photographs, representing 33 known free‐ranging bison (Bison bison), and 117 simulated data sets with varying numbers of unique individuals, morphological measurements, and photograph error. We then used the approach to estimate the adult population size of bison in Prince Albert National Park, Canada, in 2011. For bison, the false‐rejection rate of our approach was 0.055. Using a Huggins closed population model with misidentification, we estimated 103 (95% CI = 82–130) and 46 (95% CI = 37–58) adult female and male bison, respectively. After incorporating field‐based calf‐ and juvenile‐to‐female ratios, we estimated 202 (95% CI = 171.6–231.4) bison. We found this estimate to be plausible using 2 minimum‐count aerial surveys conducted in March 2011 and 2012 for comparison. With our approach, researchers and managers can build capture histories of individuals, which can be used for studies of population dynamics and habitat selection. This approach can incorporate any morphological measurements extracted from photographs (e.g., coat color), making it robust to a variety of species and study systems. © 2013 The Wildlife Society.
Effect of a by-product of solid state fermentation (Synergen™) on broiler performance
Authors: Perić L. , Spring P. 
Published: December 2013

SummaryThe trial was conducted to evaluate the effect of a by-product of solid state fermentation (Synergen™ (SGN), Alltech Inc, Nicholasville KY, USA) on broiler performance and health. One thousand two hundred and eighty male Ross 308 broilers were used in a 42 day pen trial. The trial was designed as a 2 × 2 factorial, with two diet specifications (standard and reformulated) plus or minus SGN (0 and 200 g/t, SGN replaced with commercial enzyme Ronozyme™ at 150 g/t) to give four dietary treatments in total in a corn-soy based diet formulated to commercial standards. Birds fed the reduced energy diets had significantly lower cumulative feed intakes at 42 d (P < 0.01) compared to those on the full specification standard diet. There were no significant differences in broiler body weight due to treatments at any age. Significant improvements (P < 0.05) in FCR, primarily due to SGN inclusion in the feed, were observed for all weekly reported data. There were no significant differences in either mortality or EPEF for any of the treatment diets. The present study indicates that SGN, a by-product of solid state fermentation (SSF) can improve feed conversion of broilers fed a corn-soy diet.
Transient ventilation dynamics induced by heat sources of unequal strength
Authors: Shrinivas Ajay B. , Hunt Gary R. 
Published: December 2013

AbstractWe examine theoretically the transient displacement flow and density stratification that develops within a ventilated box after two localized floor-level heat sources of unequal strengths are activated. The heat input is represented by two non-interacting turbulent axisymmetric plumes of constant buoyancy fluxes ${B}_{1} $ and ${B}_{2} \gt {B}_{1} $. The box connects to an unbounded quiescent external environment of uniform density via openings at the top and base. A theoretical model is developed to predict the time evolution of the dimensionless depths ${\lambda }_{j} $ and mean buoyancies ${\delta }_{j} $ of the ‘intermediate’ $(j= 1)$ and ‘top’ $(j= 2)$ layers leading to steady state. The flow behaviour is classified in terms of a stratification parameter , a dimensionless measure of the relative forcing strengths of the two buoyant layers that drive the flow. We find that $\mathrm{d} {\delta }_{1} / \mathrm{d} \tau \propto 1/ {\lambda }_{1} $ and $\mathrm{d} {\delta }_{2} / \mathrm{d} \tau \propto 1/ {\lambda }_{2} $, where $\tau $ is a dimensionless time. When $\hspace{0.167em} \hspace{0.167em} \ll \hspace{0.167em} \hspace{0.167em} $1, the intermediate layer is shallow (small ${\lambda }_{1} $), whereas the top layer is relatively deep (large ${\lambda }_{2} $) and, in this limit, ${\delta }_{1} $ and ${\delta }_{2} $ evolve on two characteristically different time scales. This produces a time lag and gives rise to a ‘thermal overshoot’, during which ${\delta }_{1} $ exceeds its steady value and attains a maximum during the transients; a flow feature we refer to, in the context of a ventilated room, as ‘localized overheating’. For a given source strength ratio $\psi = {B}_{1} / {B}_{2} $, we show that thermal overshoots are realized for dimensionless opening areas $A\lt {A}_{oh} $ and are strongly dependent on the time history of the flow. We establish the region of $\{ A, \psi \} $ space where rapid development of ${\delta }_{1} $ results in ${\delta }_{1} \gt {\delta }_{2} $, giving rise to a bulk overturning of the buoyant layers. Finally, some implications of these results, specifically to the ventilation of a room, are discussed.
Chaotic advection in a steady, three-dimensional, Ekman-driven eddy
Authors: Pratt L. J. , Rypina I. I. , Özgökmen T. M. , Wang P. , Childs H. , Bebieva Y. 
Published: December 2013

AbstractWe investigate and quantify stirring due to chaotic advection within a steady, three-dimensional, Ekman-driven, rotating cylinder flow. The flow field has vertical overturning and horizontal swirling motion, and is an idealization of motion observed in some ocean eddies. The flow is characterized by strong background rotation, and we explore variations in Ekman and Rossby numbers, $E$ and ${R}_{o} $, over ranges appropriate for the ocean mesoscale and submesoscale. A high-resolution spectral element model is used in conjunction with linear analytical theory, weakly nonlinear resonance analysis and a kinematic model in order to map out the barriers, manifolds, resonance layers and other objects that provide a template for chaotic stirring. As expected, chaos arises when a radially symmetric background state is perturbed by a symmetry-breaking disturbance. In the background state, each trajectory lives on a torus and some of the latter survive the perturbation and act as barriers to chaotic transport, a result consistent with an extension of the KAM theorem for three-dimensional, volume-preserving flow. For shallow eddies, where $E$ is $O(1)$, the flow is dominated by thin resonant layers sandwiched between KAM-type barriers, and the stirring rate is weak. On the other hand, eddies with moderately small $E$ experience thicker resonant layers, wider-spread chaos and much more rapid stirring. This trend reverses for sufficiently small $E$, corresponding to deep eddies, where the vertical rigidity imposed by strong rotation limits the stirring. The bulk stirring rate, estimated from a passive tracer release, confirms the non-monotonic variation in stirring rate with $E$. This result is shown to be consistent with linear Ekman layer theory in conjunction with a resonant width calculation and the Taylor–Proudman theorem. The theory is able to roughly predict the value of $E$ at which stirring is maximum. For large disturbances, the stirring rate becomes monotonic over the range of Ekman numbers explored. We also explore variation in the eddy aspect ratio.
Arrest of the flow of wet granular matter
Authors: Roeller Klaus , Blaschke Johannes , Herminghaus Stephan , Vollmer Jürgen 
Published: December 2013

AbstractWe study the arrest of three-dimensional flow of wet granular matter subject to a sinusoidal external force and a gravitational field confining the flow in the vertical direction. The minimal strength of the external force that is required to keep the system in motion, i.e. the critical force, is determined by considering the balance of injected and dissipated power. This provides a prediction whose quality is demonstrated by a data collapse for an extensive set of event-driven molecular-dynamics simulations where we varied the system size, particle number, the energy dissipated upon rupturing capillary bridges, and the bridge length at which rupture occurs. The same approach also works for systems that are kept at a fixed density by confining walls. In both cases, this universal method provides the critical force irrespective of the flow profile, and without specifying the hydrodynamic equations.
Linear stability analysis of a shear layer induced by differential coaxial rotation within a cylindrical enclosure
Authors: Vo Tony , Montabone Luca , Sheard Gregory J. 
Published: December 2013

AbstractThe generation of distinct polygonal configurations via the instability of a Stewartson shear layer is numerically investigated. The shear layer is induced using a rotating cylindrical tank with differentially forced disks located at the top and bottom boundaries. The incompressible Navier–Stokes equations are solved on a two-dimensional semi-meridional plane. Axisymmetric base flows are consistently found to reach a steady state for a wide range of flow conditions, and details of the vertical structure are revealed. An axially invariant two-dimensional flow is ascertained for small $\vert \mathit{Ro}\vert $, which substantiates the Taylor–Proudman theorem. Sufficient increases in $\vert \mathit{Ro}\vert $ forcing develops flow features that break this quasi-two-dimensionality. The onset of this breaking occurs earlier with increasing $\vert \mathit{Ro}\vert $ for $\mathit{Ro}\gt 0$ compared with $\mathit{Ro}\lt 0$. The thickness scaling of the vertical Stewartson layers are in agreement with previous analytical results. Growth rates of the most unstable azimuthal wavenumber from a global linear stability analysis are obtained. The threshold between axisymmetric and non-axisymmetric flow follows a power law, and both positive- and negative-$\mathit{Ro}$ regimes are found to adopt the same threshold for instability, namely $\vert \mathit{Ro}\vert \geq 18. 1{E}^{0. 767} $. This relationship corresponds to a constant critical internal Reynolds number of ${\mathit{Re}}_{i, c} \simeq 22. 5$. A review of reported critical internal Reynolds number and their characteristic length scales yields a consistent instability onset given by $\vert \mathit{Ro}\vert / {E}^{3/ 4} = 15. 4{\unicode{x2013}} 16. 6$; here we find $\vert \mathit{Ro}\vert / {E}^{3/ 4} = 15. 8$. At the onset of linear instability, the initially circular shear layer deforms, resulting in a polygonal structure consistent with barotropic instability. Dominant azimuthal wavenumbers range from $3$ to $7$ at the onset of instability for the parameter space explored. Empirical relationships for the preferential wavenumber have been obtained. Additional instability modes have been discovered that favour higher wavenumbers, and these exhibit structures localized to the disk–tank interfaces.
Geostrophic adjustment in a closed basin with islands
Authors: Johnson E. R. , Grimshaw R. H. J. 
Published: December 2013

AbstractWe consider the geostrophic adjustment of a density-stratified fluid in a basin of constant depth on an $f$-plane in the context of linearized theory. For a single vertical mode, the equations are equivalent to those for a linearized shallow-water theory for a homogeneous fluid. Associated with any initial state there is a unique steady geostrophically adjusted component of the flow compatible with the initial conditions. This steady component gives the time average of the flow and is analogous to the adjusted flow in an unbounded domain without islands. The remainder of the response consists of superinertial Poincaré and subinertial Kelvin wave modes and expressions for the energy partition between the modes in arbitrary basins again follow directly from the initial conditions. The solution for an arbitrary initial density distribution released from rest in a circular domain is found in closed form. When the Rossby radius is much smaller than the basin radius, appropriate for the baroclinic modes, the interior adjusted solution is close to that of the initial state, except for small-amplitude trapped Poincaré waves, while Kelvin waves propagate around the boundaries, carrying, without change of form, the deviation of the initial height field from its average.
Medical Officers, Bodies, Gender and Weight Fluctuation in Irish Convict Prisons, 1877–95
Authors: Breathnach Ciara 
Published: December 2013

AbstractThis article focuses on the function of the convict prison infirmary and views it as a site of arbitration, resistance and ‘contested power’. In accordance with the rules and regulations periods of incarceration in convict prisons began and ended with an obligatory medical examination. While the primary function of the initial test was to measure the convict body in order ascertain physical ability to conduct hard labour it also provided a thorough bio-metrical description for future identification purposes. The final examination was not as comprehensively undertaken but also concerned itself with anthropometrical observations. It would be reasonable to assume that the balance of power was weighted in the authority’s favour but this research has found evidence to the contrary. For instance, that there was a fair degree of physiological knowledge within the convict population and that some convicts used the infirmary for dietary gains and reprieve from hard labour. Using body mass index (BMI) as an instrument to measure physical wellbeing this article views the doctor–convict interface as a crucial component of the penal experience. It analyses 251 convict medical records to show that the balance of diet and work led to what might be considered a counterintuitive outcome – a preponderance of weight gain, particularly for males in Irish prisons.