bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic interactions in thalassemia intermedia: Analysis of β‐Mutations, α‐Genotype, γ‐Promoters, and β‐LCR hypersensitive sites 2 and 4 in Italian patients
Authors: Camaschella C. , Mazza U. , Roetto A. , Gottardl E. , Parzlale A. , Travl M. , Fattore S. , Bacchlega D. , Fiorelli G. , Cappellinl M.D. 
Published: November 2012

AbstractIn order to verity the genetic factors influencing the clinical expression of β‐thalassemla we have studied 292 Kalian patients, 165 with thalassemia intermedia and 127 with thalassemia major. The β‐globin gene mutations were defined in all cases. The number of α‐globin genes and the integrity of specific control regions of the β‐globin cluster—γ promoters and β‐Locus Control Region (β‐LCR)—were studied in selected cases.Homozygosity for mild mutations (group I) accounts for 24% of the intermedia patients and it is not represented among major patients. Forty‐four percent of intermedia patients had combinations of mild/severe (group II) mutations and 32% had homozygosity or double heterozygosity for severe mutations (group III). Seventy‐six percent of patients with thalassemia major were classified in group III end 24% in group II.Deletion type —α3.7 thalassemia, assessed in a part of the cases, was found in 5% of thalassemia major and 19.5% of Intermedia patients in groups II and III. Structural analysis of γ promoters and β‐LCR HS2 and HS4 regions, carried out in order to look for alterations associated with Hb F increase, did not reveal new mutations. Only rare polymorphic changes were observed at the HS2 and HS4 level. The − 158 γ C T change was found with an increased incidence in intermedia patients in groups II and III.A subset of 10 β‐thalassemia heterozygotes with mild intermedia phenotype resulted from coinheritance of a triplicated α‐locus. We have been unable to find a molecular basis for the benign clinical course in approximately 20% of patients with thalassemia intermedia. Other genetic or acquired factors must be hypothesized which ameliorate the clinical condition.
Molecular cytogenetic analysis of monosomy 7 in pediatric patients with myelodysplastic syndrome
Authors: Harrison K.J. , Massing B. , McKenna C. , Kalousek D.K. 
Published: November 2012

AbstractMonosomy 7 is a non‐random cytogenetic abnormality that is frequently associated with myelodysplastic syndromes (MDS). Twenty‐four bone marrow samples from five pediatric patients with MDS were analysed using both traditional and Interphase cytogenetic analysis. The majority of the metaphases were monosomic for chromosome 7 while interphase cytogenetic analysis consistently detected a disomic cell population in nondividing cell populations. This suggests that the monosomy 7 cells have a distinct proliferative advantage compared to the disomic cell population. The results demonstrate that interphase cytogenetic analysis provides important cytogenetic information about non‐dividing cell subpopulations, enhancing our understanding of the cell dynamics of normal and monosomy 7 cells in MDS.
Filgrastim‐mobilized peripheral‐blood stem cells can be stored at 4 degrees and used in autografts to rescue high‐dose chemotherapy
Authors: Ruiz‐Argüelles Guillermo J. , Larregina‐Díez Alejandra , Pérez‐Romano Beatrlz , Marín‐López Antonio , Larregina‐Diez Alejandra , Apreza‐Mollna M. Guadalupe 
Published: November 2012

AbstractWe studied 21 filgrastim (G‐CSF)‐mobilized peripheral blood stem cells (PBSC) apheresis products obtained from seven patients, and stored at 4°C for periods of up to 96 hr prior to their reinfusion, to rescue high‐dose chemotherapy. The apheresis products contained a median of 106 × 10/L mononuclear cells (MNC), 14.6% of them displaying the CD34 antigen; the viability was over 90% in all samples studied at 24, 48, and 72 hr after harvesting. These PBSC were successfully used to rescue high‐dose chemotherapy; patients received a median of 4.8 × 10/Kg MNC; the median time to achieve >500 granulocytes was 14 days (range 11–26) and the median time to achieve >20,000 platelets was 20 days (range 11–40). Since autologous transplants with nonfrozen PBSC are feasible and less costly than those using frozen PBSC, restrictions to PBSC autotransplant programs may be overcome and costs may be diminished.
Characterization of autoantibodies against the platelet glycoprotein antigens llb/llla in childhood idiopathic thrombocytopenia purpura
Authors: Taub Jeffrey W. , Warrier Indira , Holtkamp Christine , Beardsley Diana S. , Lusher Jeanne M. 
Published: November 2012

AbstractThe majority of children with idiopathic thrombocytopenia (ITP) have an acute self‐limiting course and no diagnostic test has been identified which will predict the course of thrombocytopenia and detect those with the chronic autoimmune form. The detection of autoantibodies directed against the platelet glycoprotein complex llB/llla, may identify patients with chronic ITP. Serum anti‐GP llb/llla antibodies were assessed by the indirect MAIPA assay in 54 children with immune thrompocytopenla at initial presentation along with an additional 7 children previously diagnosed with chronic ITP, to determine if there was a difference in antibody positivity between acute and chronic ITP patients, and whether the identification of antibodies could be used as a predictive test at diagnosis. There was no significant difference in the percentage of antibodies detected in children classified with acute ITP (27/40–68%) compared to children with chronic ITP (13/21‐62%, P > 0.05). Patients with acute ITP had significantly lower mean platelet counts at diagnosis compared to the chronic ITP group (16,225/mm3 vs. 32,250/mm3, P < 0.05), though there was no significant difference in the bleeding manifestations between the acute and chronic ITP groups. Serum anti‐GP llb/llla antibodies are detected in a high percentage of children with ITP and autoantibodies appear to be involved in the pathogenesis of both acute and chronic ITP. The detection of anti‐GP llb/llla antibodies at diagnosis, however, does not appear to be a useful prognostic test in childhood ITP. © 1995 Wiley‐Liss, Inc.
Leukodepleted‐ABO‐identical blood components in the treatment of hematologic malignancies: A cost analysis
Authors: Blumberg Neil , Heal Joanna M. , Kirkley Scott A. , Diperslo John F. , Rapoport Aaron P. , Rowe Jacob M. 
Published: November 2012

AbstractTo assess the effect of ABO‐identical, filtration leukodepleted transfusions on resource consumption and costs of care we performed a cohort study in consecutive adult patients admitted for induction therapy of acute myeloid or lymphoid leukemia during 1985–92 (n = 120) and consecutive adult patients admitted for autologous bone marrow transplantation for Hodgkin's or non‐Hodgkin's lymphoma during 1989–1991 in our university hospital. Patients with acute leukemia received either ABO unmatched, unfiltered transfusions (1985–89), ABO identical, unfiltered transfusions (1987–90), or ABO identical, filtered transfusions (1990–92). Patients with lymphoma received either ABO unmatched, unfiltered transfusions (1989–90) or ABO identical, filtered transfusions (1990–91). Mean platelet transfusion requirements per patient decreased with ABO identical platelets and filtered transfusions: from 143 to 71 units in the transplant setting; from 146 to 83 in acute leukemia (P < 0.05). Mean hospital ancillary service charges in 1992 dollars decreased with ABO identical platelets and filtered transfusions approximately $14,000 per patient for acute leukemia and $26,000 for for lymphoma. Per patient actual costs for filters ($643 in transplantation for lymphoma and $875 in leukemia) were offset by savings in actual blood component purchase costs alone ($4,127 in lymphoma and $3,283 in leukemia). In our setting introduction of ABO identical platelets and filtration leukodepletion were implemented with substantial decreases in costs. © 1995 Wiley‐Liss, Inc.
Adult T‐cell leukemia/lymphoma with two distinct clones in the peripheral blood and lymph node
Authors: Shibata Keisuke , Shimamoto Yoshinori , Suga Kenji , Watanabe Masayuki , Kikuchi Masako , Yamaguchi Masaya 
Published: November 2012

AbstractA case of adult T‐cell leukemia/lymphoma (ATL) with two different clones in the peripheral blood and lymph nodes is reported here. When cellular DNA from the lymph node was digested with EcoRI, one band larger than 9 Kb was detected. Digestion of the cellular DNA with PstI resulted in one clear band in addition to three internal fragments. In contrast, when cellular DNA from malignant peripheral blood lymphocytes (PBL) was digested with the same endonucleases, distinct bands at positions different from those observed in the lymph node were detected, indicating two separate malignant clones in the patient. Monoclonality of the tumor cells was shown by T‐cell receptor‐β (TCR‐β) gene rearrangement in both PBL and lymph node. Furthermore, there was a difference in the surface phenotype between tumor cells taken from peripheral blood (CD4+, CD8–) and lymph node (CD4+, CD8+). These findings suggest the presence of two different ATL clones in PBL and lymph node in a single patient simultaneously, which is distinguishable by the integration pattern of human T‐cell leukemia virus type I (HTLV‐I) proviral DNA.
Spurious fibrinogen levels secondary to pyroglobulinemia in Waldenström's macroglobulinemia
Authors: Cagnoni Pablo J. , Lenzi Renato , Wisch Nathaniel , Seremetis Stephanie , Rand Jacob H. 
Published: November 2012

AbstractWe report two cases of pyroglobulinemia detected in the course of routine determination of fibrinogen levels measured by the heat‐precipitation method and that led to the diagnosis of Waldenström's macroglobulinemia. The Incidence of pyroglobulinemia is briefly discussed, and the potential significance of this laboratory artifact is emphasized.
Estimating the age structure of a buried adult population: A new statistical approach applied to archaeological digs in France
Authors: Séguy Isabelle , Caussinus Henri , Courgeau Daniel , Buchet Luc 
Published: November 2012

AbstractPaleodemographers have developed several methods for estimating the age structure of historical populations in absence of civil registration data. Starting from biological indicators alone, they use a reference population of known sex and age to assess the conditional distribution of the biological indicator given age. However, the small amount of data available and the unstable nature of the related statistical problem mean that most methods are disappointing. Using the most reliable reference data possible, we propose a simple statistical method, integrating the maximum amount of information included in the actual data, which quite significantly improves age estimates for a buried population. Here the method is applied to a French cemetery used from Late Antiquity to the end of the Early Middle Ages. Am J Phys Anthropol, 2013. © 2012 Wiley Periodicals, Inc.
Patterns of violence‐related skull trauma in neolithic southern scandinavia
Authors: Fibiger Linda , Ahlström Torbjörn , Bennike Pia , Schulting Rick J. 
Published: November 2012

AbstractThis article examines evidence for violence as reflected in skull injuries in 378 individuals from Neolithic Denmark and Sweden (3,900–1,700 BC). It is the first large‐scale crossregional study of skull trauma in southern Scandinavia, documenting skeletal evidence of violence at a population level. We also investigate the widely assumed hypothesis that Neolithic violence is male‐dominated and results in primarily male injuries and fatalities. Considering crude prevalence and prevalence for individual bones of the skull allows for a more comprehensive understanding of interpersonal violence in the region, which is characterized by endemic levels of mostly nonlethal violence that affected both men and women. Crude prevalence for skull trauma reaches 9.4% in the Swedish and 16.9% in the Danish sample, whereas element‐based prevalence varies between 6.2% for the right frontal and 0.6% for the left maxilla, with higher figures in the Danish sample. Significantly more males are affected by healed injuries but perimortem injuries affect males and females equally. These results suggest habitual male involvement in nonfatal violence but similar risks for both sexes for sustaining fatal injuries. In the Danish sample, a bias toward front and left‐side injuries and right‐side injuries in females support this scenario of differential involvement in habitual interpersonal violence, suggesting gendered differences in active engagement in conflict. It highlights the importance of large‐scale studies for investigating the scale and context of violence in early agricultural societies, and the existence of varied regional patterns for overall injury prevalence as well as gendered differences in violence‐related injuries. Am J Phys Anthropol, 2013. © 2012 Wiley Periodicals, Inc.
Preparation and properties of poly(benzyl glutamate)–poloxamer–poly(benzyl glutamate) and poly(glutamic acid)–poloxamer–poly(glutamic acid) triblock polymers
Authors: Wang Xi Ting , Wang Jing , Sun Hai Long , Yu Du Xiang , Ma Li Fang 
Published: November 2012

AbstractThe novel block copolymer poly(benzyl glutamate) (PBLG)–polomamer–PBLG were synthesized from glutamic acid and poloxamer in six steps with three different molecular weights, and another new block copolymer, poly(glutamic acid) (PGA)–poloxamer–PGA, was obtained by the benzyl deprotection of PBLG–poloxamer–PBLG. The obtained compounds were characterized by IR spectroscopy, gel permeation chromatography, and 1H‐NMR. The in vitro biological degradation and water absorption of PBLG showed that a greater proportion of PBLG in the copolymer led to a slower degradation and weaker water absorption, so the speed of degradation and water absorption could be adjusted through adjustment of the ratio of poloxamer. Both PBLG–poloxamer–PBLG and PGA–poloxamer–PGA exhibited lower cytotoxicity and good biocompatibility in the methyl thiazolyl tetrazolium (MTT) assay. The results show that both block polymers are promising as drug‐carrier materials. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Controllable preparation of uniform polystyrene nanospheres with premix membrane emulsification
Authors: Liu Zhendong , Lu Yangcheng , Zhang Man , Wan Weiming , Luo Guangsheng 
Published: November 2012

AbstractThe expansion of polymer nanosphere applications requires facile and versatile preparation techniques. In this study, combining circled premix membrane emulsification and thermally initiated miniemulsion polymerization, we developed a new strategy for preparing uniform polystyrene nanospheres within a duration as short as 1 h. The size of the nanospheres, ranging from 40 to 120 nm, was dependent on the premix membrane emulsification cycle number, the transmembrane flow rate, and the membrane pore size; this was almost consistent with characterizations of droplet size evolution. The coefficient of variation, around 15%, indicated that the size distribution of the nanospheres was still narrow, even as the monomer‐to‐water ratio was as high as 0.2. This method may be competitive for further applications because of its high production efficiency and low system requirements. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Polybenzimidazole‐graft‐polyvinylphosphonic acid—proton conducting fuel cell membranes
Authors: Sinigersky Vesselin , Budurova Dessislava , Penchev Hristo , Ublekov Filip , Radev Ivan 
Published: November 2012

AbstractA new method for the preparation of polybenzimidazole (PBI)‐based membranes, containing high concentrations of immobilized phosphonic acid groups, has been developed. The procedure used is carried out in two steps: (1) Synthesis of modified PBIs, containing 1,2‐dihydroxypropyl groups and preparation of films there from; (2) Introduction of vinylphosphonic acid (VPA) and initiator (cerium ammonium nitrate) in the film, subsequent grafting of VPA from the active sites of the PBI backbone. Membranes with different length of the grafted polyvinylphosphonic acid chains were prepared. The molar ratio grafted VPA units per PBI repeating unit reaches 7.8. Proton conductivity was measured at 120°C and relative humidity (RH) 20–100%. For the membrane with highest concentration of phosphonic acid groups the proton conductivity was 35 mS cm−1 at 100% RH and 8 mS cm−1 at 20% RH. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Mechanical, thermal, and electrical conducting properties of CNTs/bio‐degradable polymer thin films
Authors: Rangari Vijaya K. , Samsur Rahman , Jeelani Shaik 
Published: November 2012

AbstractThe objective of this study is to increase mechanical, thermal, and electrical properties of plasticizer free thermoplastic bio polymer, BIOPLAST GS 2189 (BP), a blend of poly lactic acid (PLA) and potato starch. This polymer is highly suitable for sheet molding, film processing; blown film extrusion and injection molding and fully biodegradable. Structural, mechanical, thermal, and electrical properties of these films were manipulated by reinforcement of multiwalled carbon nanotubes (CNTs) in BP. Thin films of various (1–5 wt %) percentages of CNTs/BP were prepared by using a high‐speed spin coating technique. These as‐prepared films are ∼60–100 µm in thickness. The thickness measurements of these films were carried out using micrometry and optical microscopy. The maximum tensile strength (200%) and modulus (150%) was observed for 4 wt % loading of CNTs in BP as compared with the neat BP thin film. The X‐ray diffraction results show that the addition of CNTs in BP increases the crystallinity of BP. Electrical conductivity of this film also increased by 48% as compared with the neat BP polymer films. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Study on poly(D,L‐lactic) microspheres embedded in calcium alginate hydrogel beads as dual drug delivery systems
Authors: Zhong Dagen , Liu Zonghua , Xie Shasha , Zhang Wei , Zhang Yuanming , Xue Wei 
Published: November 2012

AbstractThis work is to develop a novel dual drug delivery system that can simultaneously load and release 18β‐glycyrrhetinic acid (GA, a hydrophobic drug) and bovine serum albumin (BSA, hydrophilic model drug) in a single formulation. The system consists of poly(D,L‐lactic) (PDLLA) microspheres embedded in calcium alginate hydrogel beads. The GA‐loaded microspheres were first prepared and then dispersed in the aqueous solution of sodium alginate and BSA. The resulting suspension was dropped into aqueous calcium chloride solution to obtain the dual drug delivery system. The morphology of the microspheres and beads, the drug content and loading efficiency, the interaction between GA and PDLLA, and the drug release behaviors were studied. Scanning electron microscope (SEM) revealed that the PDLLA microspheres were homogeneously distributed in the beads. Differential scanning calorimetry (DSC) measurement suggested certain interaction between GA and PDLLA, and the crystal structure of GA was influenced by the polymer. The dual release in vitro showed a rapid BSA release but a sustained GA release in all the systems. Furthermore, the release rate of BSA was accelerated by increasing PDLLA/alginate ratio, while the release rate of GA was decreased, and the release of both hydrophobic and hydrophilic drugs could be adjusted by changing the ratio of PDLLA/alginate. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 20122
Effects of montmorillonite on the structure and properties of gelatin‐polyethylene glycol composite fibers
Authors: Mu Changdao , Li Xinying , Guo Jimin , Bi Caixia , Li Defu 
Published: November 2012

AbstractTo improve the thermal and mechanical properties of gelatin‐polyethylene glycol (PEG) composite fibers, montmorillonite (MMT) is incorporated into gelatin‐PEG composite fibers during gel‐spinning process. The effects of MMT on the structure and properties of gelatin‐PEG composite fibers are investigated. The viscosimetry analysis and transmission electron microscopy measurements show that MMT has a good dispersion in spinning solution at low loading, while serious aggregation is observed with excess amount of MMT adding. The data of X‐ray diffraction (XRD) show that MMT can be intercalated and exfoliated in gelatin‐PEG‐MMT nanocomposite fibers. Fourier transform infrared (FTIR) studies show the hydrogen bonding interactions between gelatin and MMT. The mechanical properties, differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) measurements indicate that the mechanical and thermal properties of nanocomposite fibers are improved as expected at low loading of MMT. However, excess amount of MMT decreases the mechanical and thermal properties of nanocomposite fibers due to poor dispersion and clay cluster induced gelatin chains aggregation. Moreover, it is found that the mechanical properties of gelatin‐PEG‐MMT nanocomposite fibers may be more sensitive to conformational change of gelatin molecules caused by the addition of MMT. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
The body donation program at the Federal University of Health Sciences of Porto Alegre: A successful experience in Brazil
Authors: da Rocha Andrea Oxley , Tormes Diane Alícia , Lehmann Natassia , Schwab Rafael Santos , Canto Raphael Teixeira 
Published: November 2012

AbstractThe use of dissection to study human anatomy is the foundation for educational excellence among future health professionals, as it offers an ideal opportunity to learn the body's morphology in three dimensions while also providing students with a more humanistic education. The shortage of bodies for dissection, combined with the Brazilian population's lack of knowledge concerning the possibility of voluntarily donating their own bodies, led to the creation of the Body Donation Programs for Education and Research in Anatomy at the Federal University of Health Sciences of Porto Alegre (UFCSPA). The program is based on three pillars: Informing the general public about the program, donor registration, and donation itself. Since the creation of the donor program in 2008, there has been an increase in both the number of donations made during donor's lifetime and the number of bodies received by the university. There has also been a shift in relation to the origin of these bodies, as before the creation of the program most bodies were unclaimed cadavers, while today most of the bodies are sourced from voluntary donations. The initial results regarding the public's acceptance of the possibility of making body donations have been encouraging, as shown by the annual growth in donor registrations. Consequently, the quality and quantity of the material available for educational purposes have greatly improved. Anat Sci Educ. © 2013 American Association of Anatomists.
Synthesis of 1‐Arylnaphthalenes by Gold‐Catalyzed One‐Pot Sequential Epoxide to Carbonyl Rearrangement and Cyclization with Arylalkynes
Authors: Gudla Vanajakshi , Balamurugan Rengarajan 
Published: November 2012

AbstractIntermolecular and intramolecular reactions of a one‐pot gold‐catalyzed epoxide rearrangement followed by an electrophilic addition to arylalkynes to synthesize 1‐arylnaphthalenes have been reported. The intramolecular reaction has been applied to synthesize 1‐arylnaphthalenes fused with saturated furan, pyran, pyrrole, and cyclopentane ring systems.
Gold‐catalyzed Reactions of 3‐Alkynyloxireno[2, 3‐b]chromenones to Form 3(2H)‐Furanones via a Domino Pathway
Authors: Hu Feng , Yan Jianwei , Cheng Ming , Hu Youhong 
Published: November 2012

Abstract3(2H)‐Furanones are efficiently generated from 3‐alkynyl oxireno[2,3‐b]chromenones by an Au/DDQ‐catalyzed domino reaction through a pathway composed of cyclization, CC cleavage, nucleophilic addition, oxidation, and nucleophilic addition. It was found that stoichiometric AuCl3 or catalytic Au with stoichiometric DDQ can oxidize the benzylic sp3 CH bond to facilitate nucleophilic addition.