Methodology for vNOTES Sacrocolpopexy with Transvaginal Mesh Retroperitonealization and Tensioning
Authors: Shuffle Eric , Harmanli Oz , Zoorob Dani 
Published: July 2024

Background: Laparoscopy has the potential of making procedures less invasive with a lower complication profile while being more appealing to patients due to ease of recovery and cosmesis. Sacrocolpopexy has traditionally been performed through the abdominal route with limited reports of transvaginal applications. Methods: Our study aims to provide a step-by-step approach to performing a vNOTES sacrocolpopexy with appropriate tensioning and peritoneal coverage of the mesh used based on both cadaveric and live patient experience. This systematic description of a fully laparoscopic transvaginal apical suspension with mesh attachment to the sacrum being completely retroperitoneal and the mesh retroperitoneal and covered at the conclusion of the case. Results: Successful vNOTES Sacrocolpopexy is performed and reproduced, with transvaginal complete peritoneal coverage and tensioning of the mesh. Total vaginal length (TVL) of 9 cm in both live and cadaveric patients. The steps of the procedure include 1. A vNOTES laparoscopic-assisted vaginal hysterectomy is completed. 2. A 0-Prolene suture is attached longitudinally to the sacral arm of a precut (Y) mesh or any single layer Type I polypropylene mesh piece, placed from the sacral arm to the distal edge of the mesh forming a “U” shape with the bottom of the “U” ultimately attaching to the anterior longitudinal ligament. 3. To begin the SCP procedure after completion of the vaginal hysterectomy, dissection of the presacral mesh attachment site begins by entering the retroperitoneal space, slightly right of midline at the level of the ischial spine, lateral to the rectum and medial to the uterosacral ligament. 4. Once the dissection is completed with appropriate exposure of the anterior longitudinal ligament at the sacral promontory, the sacral mesh is attached to the anterior longitudinal ligament at the S1-2 level, ideally with a tacker under direct visualization. 5. Two sutures, one on each side of the tunnel, are placed on the cephalad edge of the sacral peritoneal incision and labeled. After removing the Gelport, two more absorbable sutures are attached to the anterior peritoneum of the anterior vaginal wall in the proximity of the bladder, around 3 cm on each side of the midline. These peritoneal sutures in each of the above two steps will later be tied together to help cover the arms of the mesh. 6. Before mesh arm attachment, each area on the anterior and posterior vaginal walls is dissected off respective tissue (bladder and perineal body/rectovaginal fascia) and then everted for mesh attachment. 7. Both anterior and posterior mesh pieces are sutured to their respective vaginal walls with six interrupted sutures. Each mesh arm will have several centimeters of mesh freely hanging off both anterior and posterior cuff edges. These edges can be trimmed to 2 cm past the vaginal cuff edge. 8. All three pieces of mesh are stacked together and serially threaded from anterior mesh to posterior mesh, ensuring each mesh piece remains aligned (Figure 1). Each U suture is then passed through the free ends of the anterior and posterior mesh pieces and then secured with a hemostat. The cut edges of the suture can either be aligned at the back of the posterior arm to permit retroperitonealization of the knot or placement at the vaginal apex abutting the vaginal cuff. 9. Peritoneal closure is achieved by tying a posterior tunnel suture to each respective anterior vaginal wall suture at each lateral edge of the tunnel incision. 10. The vaginal cuff closure begins with a Vicryl suture anchored at the patient’s right forniceal edge running it to midline while anchoring another suture at the left forniceal edge to permit the surgeon to tension the vaginal apex appropriately. 11. Mesh tensioning is achieved by placing a single digit through the remaining space left in the tunnel incision. A knot using the Prolene suture securing the overlapping mesh is tied extracorporeally and pushed through the vaginal cuff to mobilize the mesh crux cephalad, thus elevating the vaginal apex towards the sacrum. The elevation of this knot guides the vaginal length. 12. The remainder of the vaginal cuff is then closed while avoiding incorporation of the mesh into the suture line (the ideal distance from mesh Y-junction to cuff is 1-2cm). Conclusion: By describing an innovative and reproducible technique to perform vNOTES sacrocolpopexy based on cadaveric and live patient experience, we successfully demonstrate how to perform a laparoscopic transvaginal apical suspension with mesh attachment to the sacrum through retroperitoneal placement. The vNOTES approach for sacrocolpopexy may offer a viable alternative to the transabdominal approach for candidates with difficult transabdominal access while avoiding trocar injuries and reducing surgical costs.