bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of the electro-separation method for improvement of the utility value of winter rapeseeds
Authors: Kovalyshyn S.J. , Shvets O.P. , Grundas S. , Tys J. 
Published: October 2013

Abstract
The paper presents the results of a study of the use of electro-separation methods for improvement of the utility value of 5 winter rapeseed cultivars. The process of electro-separation of rapeseed was conducted on a prototype apparatus built at the Laboratory of Application of Electro-technologies in Agriculture, Lviv National Agriculture University. The process facilitated separation of damaged, low quality seeds from the sowing material. The initial mean level of mechanically damaged seeds in the winter rapeseed cultivars studied varied within the range of 15.8-20.1%. Verification of the amount of seeds with mechanical damage was performed on X-ray images of seeds acquired by means of a digital X-ray apparatus. In the course of analysis of the X-ray images, it was noted that the mean level of mechanical damage to the seeds after the electro-separation was in the range of 2.1-3.8%. The application of the method of separation of rapeseeds in the corona discharge field yielded a significant reduction of the level of seeds with mechanical damage. The application of the method in practice may effectively contribute to improvement of the utility value of sowing material or seed material for production of edible oil.
DMA thermal analysis of yacon tuberous roots
Authors: Blahovec J. , Lahodová M. , Kindl M. , Fernández E.C. 
Published: October 2013

Abstract
Specimens prepared from yacon roots in first two weeks after harvest were tested by dynamic mechanical analysis thermal analysis at temperatures between 30 and 90°C. No differences between different parts of roots were proved. There were indicated some differences in the test parameters that were caused by short time storage of the roots. One source of the differences was loss of water during the roots storage. The measured modulus increased during short time storage. Detailed study of changes of the modulus during the specimen dynamic mechanical analysis test provided information about different development of the storage and loss moduli during the specimen heating. The observed results can be caused by changes in cellular membranes observed earlier during vegetable heating, and by composition changes due to less stable components of yacon like inulin.
The Frequency of Allele CCR5∆32 in a Serbian Population / UČESTALOST ALELA CCR5∆32 U SRPSKOJ POPULACIJI
Authors: Đorđević Valentina , Timotijević Gordana , Pruner Iva , Radojković Dragica , Milovanović Boško , Miljković Đorđe 
Published: October 2013

Summary
Background: The mutant CCR5Δ32 allele confers resist- ance to HIV infection. Several hypotheses regarding its ori- gin and persistence in the human population have been proposed. It is assumed that the Δ32 mutation was intro- duced in Northern or Eastern Europe and that it spread to the south. Although the frequency of CCR5Δ32 was deter- mined in numerous European nations and regions, further data are needed to complete the puzzle of CCR5Δ32 dis- tribution within the continent.
Methods: To this end, CCR5Δ32 frequency was determined in a Serbian population (sample size 352). DNA was extracted from peripheral whole blood and polymerase chain reaction specific for CCR5 gene was performed. A reaction product of 263 bp was obtained from the wild- -type CCR5 sequence and a product of 231 bp was ob- tained from the truncated CCR5Δ32 sequence.
Results: Overall allele frequency of CCR5Δ32 is 4.55%; 0.57% of individuals in the examined population are homo- zygous and 8.52% are heterozygous for CCR5Δ32.
Conclusions: The determined frequency of the CCR5Δ32 allele in a Serbian population is unexpectedly low, consid- ering ethnically related populations. Keywords: CCR5, Δ32, Serbia.
The Effects of Human Wild-Type and Fals Mutant L144P SOD1 on Non-Vascular Smooth Muscle Contractions / EFEKTI HUMANE NORMALNE I FALS MUTIRANE L144P SOD1 NA NEVASKULARNE KONTRAKCIJE GLATKIH MIŠIĆA
Authors: Nikolić-Kokić Aleksandra , Oreščanin-Dušić Zorana , Slavić Marija , Spasojević Ivan , Stević Zorica , Spasić Mihajlo , Blagojević Duško 
Published: October 2013

Summary
Background: Mutated copper, zinc-containing superoxide dismutase (SOD1) may self-aggregate, an event that could also be an initial cause of motor neuron malfunction leading to disease onset. The effects of human mutated SOD1 pro- tein from the blood of familial amyotrophic lateral sclerosis (FALS) patients bearing Leu144Phe (L144F) mutation were compared to wild-type (WT) human SOD1 derived from healthy examinees, for enzymatic activity and the effects on isometric contractions of non-vascular smooth muscle.
Methods: We isolated WT and L144F SOD1 enzymes from eight patients with FALS, L144F mutation in exon 5 and eight healthy controls. We then investigated SOD1 activities in the obtained samples by the adrenaline method and pro- filed them electrophoretically. Finally, we applied WT and L144F SOD1 on the isolated rat uterus.
Results: L144F SOD1 showed lower superoxide-dismutating activity compared to WT human SOD1. We found that, in contrast to WT human SOD1, mutated L144F does not induce smooth muscle relaxation.
Conclusions: Our data suggest that the lack of relaxation of muscle tonus in the presence of mutated SOD1 may have pathogenic feedback effects in FALS.
Association of Mitochondrial DNA Variants and Cognitive Impairment of Phenylketonuria Patients / POVEZANOST VARIJANTI U MITOHONDRIJALNOJ DNK I KOGNITIVNOG FENOTIPA KOD PACIJENATA SA FENILKETONURIJOM
Authors: Klaassen Kristel , Đordevič Maja , Petrov Maja Stojiljkovič , Pavlović Sonja 
Published: October 2013

Summary
Background: Phenylketonuria (PKU) is a metabolic disorder caused by phenylalanine hydroxylase gene (PAH) mutations. If left untreated, PKU patients develop severe mental retardation potentially due to neurodegeneration. This is the first study that investigates presence of mitochondrial DNA variants in PKU patients, m.10398A, reportedly associated with neurodegenerative diseases and m.10410T.
Methods: We analyzed 64 PKU patients and 50 healthy controls from Serbian population. PKU patients were categorized into groups according to time of diagnosis and compliance to low-phenylalanine diet. The IQ was determined according to age-appropriate scales.
Results: We detected m.10398A and m.10410T variants by direct sequencing. Frequency of m.10398A was similar in patients and healthy controls (82.81% and 82.00% respectively) suggesting their identical ethnic background. No variation was detected for m.10410. In group with late diagnosis and poorly controlled diet, no statistically significant difference in average IQ was found between patients with m.10398A and m.10398G. The same was shown for PKU patients with higher IQ, diagnosed at neonatal screening and treated with low-phenylalanine diet. However, when patients carrying p.L48S, a PAH mutation with inconsistent effect, were excluded from the study, presence of m.10398A variant was associated with lower IQ.
Conclusions: This study emphasizes the importance of neonatal screening and good control of low-phenylalanine diet in PKU patients. Statistical analysis did not indicate clear impact of mitochondrial DNA variant m.10398A on IQ of PKU patients, except when PAH genotype was also considered. Studies in larger cohorts will elucidate the association between PAH gene mutations, mitochondrial DNA variants and complex PKU cognitive phenotype.
Effects of Calcium Administration on Parathyroid Gland, NaPi 2a Cotransporter and PTH1R in an Animal Model of the Andropause / EFEKTI TRETMANA KALCIJUMOM NA PARATIREOIDNU ŽLEZDU, NAPI 2A KOTRANSPORTER I PTH1R U ANIMALNOM MODELU ANDROPAUZE
Authors: Živanović Jasmina , Filipović Branko , Medigović Ivana , Ajdžanović Vladimir , Tanić Nikola , Miler Marko , Milošević Verica 
Published: October 2013

Summary
Background: Increased risk of osteoporotic bone fractures represents the adverse event in andropausal men. Due to diminished calcium absorption in elderly, its supplementa- tion is used for prevention and treatment of advanced-age osteoporosis.
Methods: Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and Ca2+- treated orchidectomized (Orx+Ca) groups. Ca2+ (28.55 mg/kg b.w.) was administered intramuscularly for 3 weeks, while the SO and Orx received vehicle alone. Parathyroid glands (PTG) were analyzed histomorphometrically, while the expression of NaPi 2a mRNA from kidneys was deter- mined by real time PCR. NaPi 2a and PTH1R abundance was detected immunofluorescently. Serum and urine parameters were determined biochemically.
Results: The PTG volume was 15% (p<0.05) greater in Orx rats than in the SO group. In Orx+Ca2+ animals, PTG vol- ume was decreased by 17% (p<0.05), when compared to the Orx rats. Orchidectomy led to an increment of serum PTH of 13% (p<0.05) compared to the SO group, while Orx+Ca decreased it by 10% (p<0.05) when compared to Orx animals. The intensity of the NaPi 2a signal was reduced in Orx rats, in comparison with the SO group. Orx+Ca2+ treatment increased the abundance of NaPi 2a, compared to the Orx group. In Orx rats, the staining for PTH1R was stronger when compared to the SO group, while the Orx+Ca2+ treatment induced reduction of the PTH1R immunofluorescence, compared to Orx animals. Orchidectomy increased Pi urinary concentrations by 8% (p<0.05), in comparison with the SO control, while in the Orx+Ca2+ group urinary Pi concentration was 5% lower (p<0.05) than for the Orx rats.
Conclusions: Our results indicate that Ca2+ administration reduces the PTH serum level and the presence of PTH1R, while increased abundance of NaPi 2a cotransporter posi- tively regulates Pi urine reabsorption in an animal model of the andropause.
The History of DNA Sequencing / ISTORIJAT SEKVENCIRANJA DNK
Authors: Gužvić Miodrag 
Published: October 2013

Summary
During the last decade, the cost of DNA sequencing technologies has decreased several orders of magnitude, with the proportional increase of speed and throughput. Human Genome Project took almost 15 years to complete the sequence of the human genome. With the second and third generation technologies, this can be done in the matter of days or hours. This progress and availability of sequencing instruments to virtually every researcher leads to replacing of many techniques with DNA sequencing and opens new venues of research. DNA sequencing is used to investigate basic biological phenomena, and is probably going to be increasingly used in the context of health care (preimplantation diagnostics, oncology, infectious diseases). Current trends are aiming towards the price of 1000$ for sequencing of one human genome. Without any doubt, we can expect improvement of existing and the development of fourth generation technologies in the coming years.
The Impact of Immediate Verbal Feedback on Swimming Effectiveness
Authors: Zatoń Krystyna , Szczepan Stefan 
Published: October 2013

Abstract The main objective of the study was to determine the impact of immediate verbal feedback on swimming effectiveness. Swimming effectiveness was expressed in the subjects reaching their objective, i.e., maximum swimming velocity. The study involved 64 subjects divided into two groups (experimental group n=32; control group n=32). Two measurements - initial (pre-test) and final (post-test) - were conducted. The subjects swam a distance of 25 m front crawl at maximum velocity. The experimental groups received immediate verbal feedback focused on stroke lengthening, as a shorter stroke length is regarded as the most common reason behind lower swimming velocity. From this perspective, lengthening the stroke is important due to its relation with mechanics and energetics in all styles of swimming. The control group received no verbal feedback. All tests were recorded via video cameras (50 samples·s-1). The analysis of the kinematic movement parameters (horizontal average swimming velocity over 15m, time achieved over 15m) was carried out by means of the SIMI Reality Motion Systems 2D software. Analysis of variance with repeated measurements with a Tukey’s test demonstrated statistically significant differences (p<0.05) in the tested groups in the case of the kinematic parameters measured in the study. Immediate verbal feedback (IVF) caused an increase of the average horizontal swimming velocity by 2.92% (0.04 m·s-1). Consequently, the average time needed by the swimmers to cover the distance (15m) decreased by 2.94% (0.36s). The results confirmed the effectiveness of the proposed method of teaching and improving the swimming technique using IVF.
Italian Early Adolescent Females’ Intrinsic Motivation in Sport: An Explorative Study of Psychological and Sociorelational Correlates
Authors: Zucchetti Giulia , Candela Filippo , Rabaglietti Emanuela , Marzari Alessandra 
Published: October 2013

Abstract Using self-determination theory (SDT), this study simultaneously investigated the relationships between intrinsic motivation, psychological factors (sport enjoyment, social self-efficacy), and sociorelational factors (perceived coaching behaviors) by subsequently testing whether psychological factors mediate the relationships between social-relational factors and intrinsic motivation. One hundred and twentyseven early adolescent females from several teams of the Italian Volleyball Society completed a self-report questionnaire. Results showed that female athletes’ intrinsic motivation was associated with a) sport enjoyment and b) perceived coaching behaviors (e.g., training and instruction, social support). Second, results showed that training-and-instruction coaches’ leadership style affected intrinsic motivation via the females’ enjoyment in sport. This study provided further insights into unknown associations among psychological, sociorelational factors and intrinsic motivation, highlighting the innovative role of sport enjoyment as a key factor for sustaining intrinsic motivation. Original and useful behavioral suggestions will be given to the coaches in order to sustain female athletes’ sport enjoyment and their intrinsic motivation.
Application of Inclinometer Measurements for Relative Horizontal Displacement Investigations on Landslide Grounds
Authors: Malarski Ryszar , Nagórski Kamil , Woźniak Marek 
Published: October 2013

Abstract One of the basic criterion for safety evaluation of structures erected on embankment or sliding slopes is relative horizontal displacement at different elevations. Relative horizontal displacements beneath ground surfaces are performed with inclinometer measurements. This report presents results on horizontal relative ground displacements with the probe SISGEO S242SV30. Investigations were performed with two inclinometer columns 14m height embedded in Warsaw Bank Slope. Mean square error of single observation was determined and mean relative errors of relative displacements in relation to column height. On the basis measurement results several relevant recommendations and practical hints were formulated allowing to avoid survey blunders and discrepancies in measuring procedures often encountered. Detailed inclinometer horizontal displacement measurements results at Warsaw hillside St Ana’s Church grounds were listed and presented.
Prospects of Metrological Provision Linear Geodetic of Measurements on the Geodetic Test Field
Authors: I.S. Trevoho , I.M. Tsyupak 
Published: October 2013

Abstract This paper gives brief information on existing metrological support for the Yavoriv of scientific of the geodetic of test field (SGTF) for testing of modern of surveying equipment (rangefinders, electronic total stations and GNSS receivers). Analyzed ways to improve the fundamental geodetic network and the standard linear of basis, as working standards for testing, in accordance, GNSS receivers and a rangefinders, as well as for study an accuracy technology of GNSS leveling.
Generalized model for the nonlinear dynamics of plasma waves driven unstable by energetic ions near the stability threshold
Authors: Galant Grzegorz 
Published: October 2013

Abstract
In this paper, an attempt to develop the original single mode theory describing the nonlinear dynamics of a linearly unstable plasma wave, excited by the resonant interaction with energetic ions near the stability threshold to the case of n interacting plasma modes has been made. The effects of an energetic ion source and classical collisional processes represented by the Krook, diffusion and dynamical friction (drag) collision operators are included in the model. For numerical purposes, the problem has been reduced to ten nonlinearly coupled integro-differential equations. In comparison to the previous papers, the system revealed similar (the steady-state, oscillation, and blow-up solutions), as well as quite new types of the amplitudes behaviour, i.e. different levels of competition between the modes.
A Multisport Epidemiologic Comparison of Anterior Cruciate Ligament Injuries in High School Athletics
Authors: Joseph Allan M. , Collins Christy L. , Henke Natalie M. , Yard Ellen E. , Fields Sarah K. , Comstock R. Dawn 
Published: October 2013

Background:
The knee joint is the second most commonly injured body site after the ankle and the leading cause of sport-related surgeries. Knee injuries, especially of the anterior cruciate ligament (ACL), are among the most economically costly sport injuries, frequently requiring expensive surgery and rehabilitation.

Objective:
To investigate the epidemiology of ACL injuries among high school athletes by sport and sex.

Design:
Descriptive epidemiology study.

Main Outcome Measure(s):
Using an Internet-based data-collection tool, Reporting Information Online (RIO), certified athletic trainers from 100 nationally representative US high schools reported athlete-exposure and injury data for athletes from 9 sports during the 2007/08–2011/12 academic years. The outcome of interest in this study was ACL injuries.

Results:
During the study period, 617 ACL injuries were reported during 9 452 180 athlete exposures (AEs), for an injury rate of 6.5 per 100 000 AEs. Nationally, in the 9 sports studied, an estimated 215 628 ACL injuries occurred during the study period. The injury rate was higher in competition (17.6) than practice (2.4; rate ratio [RR] = 7.3, 95% confidence interval [CI] = 6.08, 8.68). Girls' soccer had the highest injury rate (12.2) followed by boys' football (11.1), with boys' basketball (2.3) and boys' baseball (0.7) having the lowest rates. In sex-comparable sports, girls had a higher rate (8.9) than boys (2.6; RR = 3.4, 95% CI = 2.64, 4.47). Overall, 76.6% of ACL injuries resulted in surgery. The most common mechanisms of injury were player-to-player contact (42.8%) and no contact (37.9%).

Conclusions:
Anterior cruciate ligament injury rates vary by sport, sex, and type of exposure. Recognizing such differences is important when evaluating the effectiveness of evidence-based, targeted prevention efforts.
De la capacité, de la compétence, de l’empowerment, repenser l’anthropologie de la participation
Authors: Genard Jean-Louis 
Published: October 2013

Le plus souvent étudiée dans le contexte des évolutions du politique et du passage présumé d’une démocratie représentative vers une démocratie davantage participative ou délibérative, la question de la participation est abordée ici sous l’angle d’une transformation de nos coordonnées anthropologiques. Les exigences et les attentes de participation se retrouvent en effet dans de multiples domaines qui excèdent de loin la seule sphère du politique, allant du monde de l’entreprise et de ses cercles de qualité jusqu’à la production de biens en kit sollicitant les capacités de montage des acheteurs. Cette extension du domaine de la participation s’appuie en réalité sur un certain nombre de présupposés anthropologiques. Ceux-ci prêtent aux acteurs descapacitéset descompétencesqu’ils sont censés détenir, à moins qu’ils ne soient appelés à les enrichir par des stratégies d’empowermentsi elles leur font défaut ou sont insuffisantes. Ils leur attribuent aussi un potentiel demotivationindispensable à l’actualisation de ces compétences et capacités. Le texte soutient que l’évaluation des personnes ainsi que la configuration de nombreux dispositifs sociaux récents tendent de plus en plus à s’ajuster à ce nouveau référentiel anthropologique. Il en va ainsi de la participation politique bien sûr, mais aussi des nouvelles politiques sociales, des formations au « pouvoir d’agir », des objectifs éducatifs, des politiques de développement ou de santé. Revenant plus spécifiquement sur la question de la participation politique, le texte montre comment ces nouvelles coordonnées anthropologiques reconfigurent la question de l’expertise en réorganisant les partages entre expertises ordinaire et spécialisée et, du coup, le partage entre les espaces où peut prendre place une démocratie participative et ceux qui demeurent confiés à l’expertise technocratique.
Deux conceptions de l’empowerment
Authors: Cantelli Fabrizio 
Published: October 2013

La catégorie d’empowermentdevient omniprésente quand il s’agit de participation. Pourtant, peu de travaux en ont exploré sociologiquement les contours, les propriétés et la portée. Il s’agit de prendre distance avec une approche unidimensionnelle de la catégorie, qui l’appréhende comme une solution démocratique ou une régression liée à un usage managérial. Notre démarche comparative élargie considère plusieurs espaces-temps, ce qui permet de rendre compte de deux manières très différentes de penser le pouvoir et d’agir sur des capacités. Cet article interroge deux conceptions de l’empowerment, soit deux philosophies du pouvoir sous-jacentes : une gestionnaire et une civique. Pour saisir les conceptions gestionnaires de l’empowerment, notre article s’appuie sur un spectre allant du cas spécifique de la Banque mondiale (Empowerment Team) à plusieurs acteurs de la santé en relation avec les institutions européennes. Concernant les conceptions civiques de l’empowerment, l’enquête repose sur le mouvement des droits civiques aux États-Unis et leBlackpowerdans les années 1960, tout en s’interrogeant sur l’héritage contemporain.
Elevated IgG4 Serum Levels Among Primary Sjögren's Syndrome Patients: Do They Unmask Underlying IgG4‐Related Disease?
Authors: Mavragani Clio P. , Fragoulis George E. , Rontogianni Dimitra , Kanariou Maria , Moutsopoulos Haralampos M. 
Published: October 2013

ObjectiveTo determine IgG4 levels in a cohort of consecutive patients with primary Sjögren's syndrome (SS) and other autoimmune diseases and explore whether they associate with distinct clinical, serologic, and histopathologic features.MethodsSerum IgG4 levels were measured in 133 primary SS patients and 49 healthy donors (HDs). Seventy‐four lupus and 54 rheumatoid arthritis (RA) patients served as disease controls. Immunohistochemical IgG4 analysis was performed in paraffin‐embedded minor salivary gland (MSG) tissues.ResultsRaised IgG4 serum levels (>135 mg/dl) were detected in 10 (7.5%) of 133 primary SS patients (high‐IgG4 group), in 8 (10.8%) of 74 lupus patients, in 7 (12.9%) of 54 RA patients, and in 1 (2%) of 49 HDs. Compared to the normal‐IgG4 (<135 mg/dl) primary SS group, high‐IgG4 patients exhibited increased prevalence of IgG4‐related features (autoimmune cholangitis, autoimmune pancreatitis, and interstitial nephritis), lower rates of antinuclear antibody positivity, and higher IgG2 and IgE levels. Positive staining for IgG4+ plasma cells with an IgG4:IgG ratio ≥40% was detected in 3 of 6 available MSG tissues in the high‐IgG4 group. Criteria of possible or definite IgG4‐related disease (IgG4‐RD) were fulfilled by 10 (7.5%) of 133 of our primary SS cohort.ConclusionRaised IgG4 serum levels were detected in 7.5% of primary SS patients in association with IgG4RD–reminiscent clinical, serologic, and histopathologic features. Whether this high‐IgG4 primary SS group represents a misclassified IgG4‐RD group or a distinct primary SS subtype remains to be further explored in future studies.