bluuebunny/crossref_metadata_2025_split huggingface dataset:
ULTRASTRUCTURAL LOCALIZATION OF 2,4‐DINITROPHENYL GROUPS ON DRAINING LYMPH NODE CELLS OF GUINEA PIGS FOLLOWING SKIN PAINTING WITH 2,4‐DINITROCHLOROBENZENE
Authors: Nakaye Masako , Nakagawa Shojiro , Ueki Hiroaki , Kumon Hiromi , Ohmori Hiroyuki 
Published: April 2015

ABSTRACTThe distribution of DNP groups on cells taken from draining lymph nodes of guinea pigs 12 hours after painting the skin with DNCB was examined by scanning immunoelectron microscopy using antibody directed against DNP groups with bacteriophage T4 as a visual marker. The studies showed that DNP groups were distributed on the surface of the cells, demonstrating some differences in distribution pattern among the cells. The incidence of cells on which DNP groups were detected was approximately 60% in the normal animals, higher than that detected by a previous immunofluorescent study. The incidence was markedly reduced to approximately 3% by pretreatment with tolerogen, DNBSO3Na. DNP groups were detected on the surface of the cells forming both rabbit red cell rosettes and complement coated red cell rosettes. It is suggested that DNCB penetrates through the skin into the lymphatics carrying lymph from the painted site and combines with cell membrane components in the peripheral lymphoid system.
CHARACTERIZATION OF RIBOSOMAL PROTEINS FROM STAPHYLOCOCCUS AND MICROCOCCUS BY TWO‐DIMENSIONAL GEL ELECTROPHORESIS
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

ABSTRACTThe proteins of 70S ribosomes from Staphylococcus aureus Smith (diffuse), Staphylococcus epidermidis IAM 12012, and Micrococcus luteus (lysodeikticus) IFO 3333 were analyzed by two‐dimensional polyacrylamide gel electrophoresis. Although the electrophoregram of the ribosomal proteins from Staphylococcus aureus was completely identical to that from Staphylococcus epidermidis, it was very different from the electrophoregram from Micrococcus luteus. These results are considered to indicate that Staphylococcus is phylogenetically very different from Micrococcus.
TWO CASES OF NEVUS FUSCOCAERULEUS WITH PAIN, INCLUDING A CASE OF PILAR NEUROCRISTIC HAMARTOMA
Authors: Kikuchi Ichiro , Inoue Shouhei , Taketomi Isao , Ono Tomomichi 
Published: April 2015

ABSTRACTTwo patients with nevus fuscocaeruleus over the back and simultaneous pain were reported. Although the significance of this association remains to be clarified, it is interesting because the dermal melanocytes and nerves are of neuroectodermal origin. Histology of one case revealed findings consistent with those of pilar neurocristic hamartoma (1). Electron microscopy revealed dermal melanocytes in the neighborhood of a nerve. Photographic studies confirmed Morikawa's view (2) that the location of melanin may be examined by reflection ultraviolet photography, conventional photography, and infrared photography.
EFFECTS OF BETAMETHASONE 17 VALERATE ON THE CYCLIC AMP SYSTEM OF THE PIG SKIN EPIDERMIS
Authors: Ishizawa Hideaki , Iizuka Hajime , Kajita Satoshi , Ohkawara Akira 
Published: April 2015

ABSTRACTIt has been reported that hydrocortisone had a protective effect on beta‐adrenergic adenylate cyclase system of the pig epidermis during in vitro long term incubation. Using the same experimental system, the effects of another glucocorticoid, betamethasone 17 valerate were investigated. In short term experiments (5 min incubation time), betamethasone had no effect on the cyclic AMP accumulation of the epidermis. When skin slices were incubated with this compound for 48 hours, the response of the skin to epinephrine was preserved and showed a greater accumulation of cyclic AMP in betamethasone‐treated skin than in the control. This effect was observed at concentrations greater than 0.001 μM of betamethasone, with the maximal accumulation of cyclic AMP at 0.1 μM. Neither histamine responsiveness nor cyclic AMP phosphodiesterase activities (low and high Km enzymes) were altered by the betamethasone treatment. Thus, betamethasone showed a protective effect against the beta‐adrenergic adenylate cyclase system similar to that of hydrocortisone in much lower concentrations.These effects of glucocorticoids would be the essential pharmacological action of this type of hormone on epidermis, and glucocorticoids might reveal their biological activity through the cyclic AMP system of the skin.
A MECHANISM OF TRANSIENT IMMUNE DEPOSITION IN THE SKIN
Authors: Masuda Toshiki , Ueki Hiroaki , Nakaye Masako , Hatamochi Atsushi 
Published: April 2015

ABSTRACTTo gain further insight into the mechanism of immune deposition in the skin, antigens and antibodies were administered intravenously at different times into normal guinea pigs, and the presence of immune complexes in frozen sections of skin was determined immunohistochemically, using light microscopy. Cutaneous immune deposits were found after primary injection of anti‐horseradish peroxidase antibody (IgG) followed by secondary administration of horseradish peroxidase, while no immune complexes were detected after the same injections in the reverse order. This disparity may possibly be explained by the length of time the antigen or antibody remains in the skin. Using a primary injection of bovine serum albumin (BSA), followed by secondary injection of anti‐BSA antibody, immune deposits were demonstrated in the skin. In these cases, if the initially injected antigen or antibody remained for a long time at the site of exudation, they would react with and form immune complexes with the subsequently injected antibody or antigen at the same site.
EPIDERMOLYSIS BULLOSA ACQUISITA DIAGNOSED BY IMMUNOELECTRON MICROSCOPY
Authors: Matsukawa Ataru , Furue Masutaka , Yu Hsin‐Su , Tamaki Kunihiko , Yaoita Hideo 
Published: April 2015

ABSTRACTA 60‐year‐old Japanese man with a five year history of bullous lesions associated with scar and milia is described. This case seemed to have an unusual type of bullous pemphigoid clinically, histologically and immunofluorescently, but was found to have epidermolysis bullosa acquisita (EBA) based on electron microscopic and immunoelectron microscopic findings, in which amorphous substance and immunological reaction products were detected mainly below the subbasal lamina‐anchoring fibril zone.
ACQUIRED COLD URTICARIA
Authors: Katayama Ichiro , Doi Toshiaki , Nishioka Kiyoshi , Maeyama Kazutaka , Yamatodani Atsushi 
Published: April 2015

ABSTRACTThree patients with idiopathic cold urticaria were treated with either or both cyproheptadine (Histamine H1 receptor antagonist) and cimetidine (Histamine H2 receptor antagonist).Cold induced histamine release (CIHR) was significantly higher in the patients (71.0 ± 23.8 pm/ml) than in normal volunteers (4.5 ± 1.0 pm/ml). Improvement of clinical manifestations with reduced CIHR was obtained by combination therapy using cyproheptadine and cimetidine but no favorable response was noted with either drug alone. The combined therapy with both drugs completely suppressed pruritis and edema but failed to suppress the erythematous reaction. Single cyproheptadine therapy was rather favored by the patients despite positive CIHR with the therapy.Histamine release from patients' leukocytes under three different conditions (37°C, 4°C→37°C, 4°C) was not significantly different.
EFFECTS OF ORAL RETINOID ON THE SDS‐PAGE PROFILE OF STRATUM CORNEUM KERATIN IN PATIENTS WITH PSORIASIS
Authors: Kitajima Yasuo , Tsuneda Yoriko , Furuta Hiroko , Mori Shunji 
Published: April 2015

ABSTRACTα‐Keratins from horny cells of lesional and non‐lesional skin in psoriatic patients were examined by sodium dodecylsulfate polyacrylamide gel electrophoresis before and after oral retinoid (etretinate at a dose of 50 mg/day) treatment. Horny cell specimens were obtained from lesional, non‐lesional, and normal human skin of the back or thigh by scraping with scalpel and/or tape stripping before retinoid treatment. Horny cell specimens of healed‐lesional skin and retinoid‐affected skin were obtained by tape stripping because the horny cell layer was too thin to use a scalpel. α‐Keratins from lesional horny cells showed 3 major polypeptide bands with molecular weights (mw) of 53,000, 55,000, and 59,000 and some minor bands with mw of 62,000, 66,000, and less than 53,000. Non‐lesions showed two polypeptides (mw, 59,000 and 66,000) before treatment. After treatment, α‐keratins from horny cells of both non‐lesions and resolved lesions showed three polypeptides bands (mw, 59,000, 62,000, and 66,000).
SERUM LEVELS OF ETRETINATE (RO 10‐9359) AND ITS METABOLITE (RO 10‐1670)
Authors: Ishizaki Hiroshi , Masuda Hiroshi , Kaseda Hidenori , Kaminaga Tokio , Hirone Takae 
Published: April 2015

ABSTRACTSerum levels of Etretinate (ET, Ro 10‐9359) and its metabolite (AM, Ro 10‐1670) were monitored during and after cessation of ET treatment in 12 patients with acquired disorders of keratinization who underwent the ET therapy at initial doses of 30 to 60 mg/day. Studies were made of the possible correlations between ET and AM serum levels, the appearance and disappearance of side effects, and elimination time profiles. Side effects appeared 1 to 2 weeks after the initiation of ET administration in all cases, although serum ET and AM levels at this time varied considerably, indicating individual differences in ET and AM susceptibility in the appearance of side effects. Serum ET and AM levels at the time of disappearance of side effects were lower than those at the time of appearance, suggesting some correlation of side effects with serum levels of ET, AM, or both. Serum level curves for ET and AM after cessation of ET administration were diverse. The maximum ET and AM elimination times from serum were 3 and 6 months, respectively.
DERMATOLOGICAL SURVEY OF ONCHOCERCIASIS IN GUATEMALA
Authors: Nonaka Shigeo , Yoshimura Takesumi , Sakamoto Makoto , Flores Guillelmo Zea 
Published: April 2015

ABSTRACTA comprehensive survey of onchocerciasis was made at San Vicente Pacaya, Guatemala, in 1978 to determine whether a relationship exists between general cutaneous changes and specific ones in Guatemalan onchocerciasis, and whether the depigmentation on the lower extremities is an onchocercal specific change or not. A total of 1185 people, 623 males and 562 females, were examined. The prevalence rate of onchocerciasis in the area surveyed was 41.6% in males and 17.3% in females. The prevalence rate of nodules was 20.1% in males and 6.8% in females. There was a similarity in the age distribution curves between the prevalence rate of nodules and that of onchocerciasis. The prevalence rate of the depigmentation on the lower extremities was 4.8% in males and 6.4% in females. There was no correlation between the age distribution curve and the prevalence rate of onchocerciasis. Symptoms coincident to the so called “pretibial depigmentation” were seen in 5 of 66 depigmented cases. The prevalence rate of the cicatricial depigmentation on the lower extremities was 31.3% in males and 28.3% in females. The rate of this depigmentation was considerably higher than the non‐cicatricial depigmentation of the lower extremities.The prevalence rate of lymphadenopathy was 59.8% in males and 9.8% in females. “Hanging groin” and elephantiasis were not seen in this region of Guatemala. The prevalence rate of all eczematous dermatitis was 11.3% in males and 9.8% in females. There were six cases with generalized eczematous dermatitis among our series of 128 cases. Five cases were seen in patients with onchocerciasis. Except for lymphadenopathy, our data shows the prevalence rate of cutaneous changes to be relatively low when compared to the prevalence rate of Guatemalan onchocerciasis. The prevalence rate of the generalized eczematous dermatitis, which may be similar to onchocercal dermatitis, is extremely low, and the depigmentation on the lower extremities, which may be similar to the pretibial depigmentation, is not commonly seen in this region of Guatemala. However, similar mild conditions not coinciding with the typical case showed a considerably higher prevalence rate in the same surveyed region. The correlation between the prevalence rate of the lymphadenopathy and the onchocerciasis infection rate appeared to be real. From these results, we feel that palpation of lymph nodes is useful for the determination of clinical information in onchocerciasis, without causing any significant discomfort to the patients being surveyed.
FAILURE TO DETECT β2 MICROGLOBULIN IN VIRAL WARTS
Authors: Furue Masutaka , Inoue Yukiko 
Published: April 2015

ABSTRACTTwenty warts including Tinea versicolor like lesions of epidermodysplasia verruciformis were examined for cell membrane associated β2 microglobulin by the use of the direct immunofluorescence and indirect immunoperoxidase technique. The human papilloma virus infected keratinocytes in the granular layer and the uppermost stratum spinosum lack immunoreactive β2 microglobulin on the cell surface. This finding indicates that the human papilloma virus does alter the cell membrane properties of the infected target keratinocyte, and the failure to detect β2 microglobulin on cell surface does not necessarily mean that these cells have undergone malignant transformation.
A STUDY OF THE INTERACTION BETWEEN CHLORPROMAZINE AND RIBOFLAVIN BINDING PROTEIN
Authors: Shiga Akiko , Matsumoto Syuichi , Ueda Hiroshi , Shiga Kiyoshi , Nishina Yasuzo , Watari Hiroshi , Horiike Kihachiro 
Published: April 2015

ABSTRACTThe interaction between chlorpromazine (CPZ) and riboflavin binding protein (RBP) was studied by the spectrophotometric and spectrofluorometric methods.CPZ combined with RBP in a 1:1 molar ratio and the dissociation constant was determined to be 3.8 × 10–5M at pH 7, 25°C. Flavins such as riboflavin (Rf) and lumiflavin were released from the complexes with RBP by the addition of CPZ. The mechanism of flavin‐release was mainly competitive binding between CPZ and a isoalloxazine ring of flavins to RBP.The mechanism of some biological phenomena induced by CPZ was discussed based on the flavin‐CPZ interaction.
NATURAL KILLER CYTOTOXICITY OF HUMAN LYMPHOCYTES IN VITRO
Authors: Kondo Shigeo , Sato Kumi , Aso Kazuo 
Published: April 2015

ABSTRACTThe cytotoxicity of allogenic human lymphocytes was measured by 51Cr release from the target cells (HSC‐1). The null cell fraction as well as the T cell fraction treated with anti‐T cell monoclonal antibody showed cytotoxicity against the target cells; this was not enhanced by the addition of monocytes. It appeared therefore that the observed killing was natural killer‐like cytotoxicity. Enhancement of the cytotoxicity was observed when OK‐432 was present in the culture of lymphocytes.
GENERALIZED PUSTULAR PSORIASIS AND BACTEREMIA
Authors: Matsubara Motoo , Komori Machio , Koishi Kazuo , Yasuno Hirokazu , Ueda Keiichi , Seto Yoshifumi , Nonomura Kyoko 
Published: April 2015

ABSTRACTA 73‐year‐old man, who developed bacteremia in the course of generalized pustular psoriasis (GPP) which appeared one year after the onset of psoriasis vulgaris, was reported. Blood culture confirmed bacteremia caused by Staphylococcus aureus, Epidermidis and Streptococcus faecalis. We emphasized in this article that bacteremia should be considered in emaciated patients with GPP who have received corticosteroid therapy for a long time, show remarkable shift to the left of neutrophils, and suffer remittent fever.
A CASE OF SUBACUTE CUTANEOUS LUPUS ERYTHEMATOSUS WITH A HIGH TITER OF RNP ANTIBODY
Authors: Kobayashi Yoichi , Matsunaga Etsuji , Nishioka Kiyoshi , Sano Shigeharu 
Published: April 2015

ABSTRACTA 30‐year‐old man developed severe erosive lesions in his oral cavity and transient Raynaud's phenomenon followed by discoid lupus erythematosus (DLE)‐like lesions and non‐scarring erythema. He had a high titer of RNP antibody as well. Widespread DLE (w‐DLE), subacute cutaneous lupus erythematosus (SCLE) and mixed connective tissue disease (MCTD) were suspected as his condition. Finally we diagnosed him as having SCLE accompanied with a high titer of RNP antibody.
ULTRASTRUCTURE OF FIBROUS PAPULE OF THE NOSE
Authors: Kimura Shunji , Yamasaki Yuichiro 
Published: April 2015

ABSTRACTThree cases of fibrous papule of the nose (FPN) were studied by electron microscopy. All three showed similar ultrastructural features; in the overlying epidermis, a number of large and small vacuoles without limiting membranes were observed within the keratinocytes, and melanocytes and melanosome‐complexes in the keratinocytes were increased in number; in the dermis, a number of mono‐ and binuclear mesenchymal cells which may well be regarded as fibrohistiocytic cells, proliferation and dilation of capillaries and venules, and a considerable number of Zebra bodies were seen. Neither melanocytes nor nevus cells were found in the dermis. These findings suggest that the large pale cells seen in the epidermis by light microscopy are vacuolated keratinocytes and that FPN has characteristics not of a nevocellular nevus but of an angiofibromatous tumor similar to so‐called adenoma sebaceum of Pringle's disease.
A BIOCHEMICAL STUDY OF EXPERIMENTAL PORPHYRIA
Authors: Shimoyama Tokio , Nonaka Shigeo , Honda Tetsuzo , Murayama Fumio , Ohgami Taro , Yoshida Hikotaro 
Published: April 2015

ABSTRACTIn order to determine the lowest concentration of griseofulvin (GF) needed to induce abnormal porphyrin metabolism, D‐D strain mice were fed with a feed containing GF in concentrations of 0.1%, 0.5%, and 1.0%. The liver and blood porphyrin levels were analyzed, and the red fluorescence of the liver and blood observed with a fluorescent microscope. In the 0.5% GF and 1.0% GF groups, a swelling of the liver was observed, and coproporphyrin and protoporphyrin levels in the liver and the blood increased markedly. However, the increase in protoporphyrin levels was more prominent than the increase of coproporphyrin levels. The increase in the liver protoporphyrin was more marked than that in the blood porphyrin. Comparisons of the 0.5% GF and 1.0% GF groups revealed that liver swelling was more prominent in the 1.0% GF group. A high degree of metabolic abnormality in blood protoporphyrin was found in 1.0% GF animals whose feeding period was rather short. In the 0.1% GF group, liver swelling was hardly noticeable, and there were no differences between the short feeding and long feeding groups. Although no abnormalities in blood porphyrins were noticed in comparison with the normal group, abnormally high levels of liver porphyrins were found in 3 out of the 34 treated mice. No differences from the normal group were noted in the remaining 31 animals. In the 0.5% GF and 1.0% GF groups, red fluorescence of the liver was seen in all cases, while in the 0.1% GF group, reticular red fluorescence was noted in only one animal.From these findings, it appears that a marked increase in porphyrin occurs at a concentration above 0.5% in D‐D strain mice, whereas, at the concentration of 0.1%, the majority of the treated mice remain within normal limits. Only a few showed any abnormality of porphyrin metabolism. We feel that, for this reason, it would be better to use a GF concentration of 0.1% for the lowest concentration experiments involving GF‐induced protoporphyria in D‐D strain mice and especially for investigations of the interaction of other chemicals with GF, and investigations of initial changes of porphyrin metabolism.
IMMUNOCYTOCHEMICAL STUDIES ON INFLAMMATORY INFILTRATES IN PSORIASIS
Authors: Kaneko Fumio , Kawagishi Ikuro , Miura Yusho , Watanabe Makoto , Kishiyama Kazunori , Ohkawara Akira 
Published: April 2015

ABSTRACTDeposits of immunoglobulins (Igs) and complement components can be demonstrated in the stratum corneum (SC) of psoriatic lesions by immunofluorescent (IF) technique. In this paper, the underlying inflammation in the lesional dermis, which may affect the immune reactions in the SC, was investigated by immunocytochemical and IF techniques.Using the reactive tests for α‐naphthyl acetate esterase (ANAE) activity, T‐lymphocytes were found to be predominant among inflammatory infiltrates in the lesional dermis. By the immunoperoidase method, IgA and secretory component (S‐component) bearing cells, which seemed to be bound to each other on their cell surfaces, comprised about 20% of the mass of infiltrates in the upper dermis of early psoriatic lesions. These Igs and S‐component bearing cells seemed to be non T‐lymphocytes.The inflammatory conditions in the dermis appeared to be closely connected to the immune reactions in the SC of psoriasis.
COEXISTENCE OF UNUSUAL SCABIES AND PNEUMOCYSTOSIS IN A PATIENT WITHOUT ANY UNDERLYING DISEASE
Authors: Arata Jirô , Ohara Atsunobu , Yamamoto Yasuo , Ikeda Masami , Kobayashi Makoto 
Published: April 2015

ABSTRACTAtypical and uncommon scabies with innumerable burrows was seen in a patient with Pneumocystis carinii pneumonia who had no underlying disease or apparent immune deficiency. Some unknown immunological abnormality was considered to underlie both the development of Pneumocystis carinii pneumonia and the modification of scabies in this patient.
LICHEN PLANUS AMONG ARABS—A STUDY FROM LIBYA
Authors: Kanwar A.J. , Belhaj Mohammed Suleiman 
Published: April 2015

ABSTRACTAn analytical study of 134 patients with lichen planus is presented. The sex ratio was 1.6:1 (M:F) and most patients having the disease were in the age group from 31 to 50 years. Lichen planus vulgaris (common type) was seen in 97 (72.39%) patients, lichen planus hypertrophicus in 15 (11.19%), lichen planus actinicus in 8 (5.97%), lichen planus follicularis and lichen planus atrophicus each in 3 (2.24%) and lichen planus pemphigoides in 1 patient. Mucous membrane involvement was seen in 19 (14.18%) patients and nail changes in only 3 (2.23%).
EPIDERMAL LANGERHANS CELLS IN VARIOUS SKIN DISEASES (2)
Authors: Kano Yoko , Shiohara Tetsuo , Nagashima Masaji 
Published: April 2015

ABSTRACTWe compared the density of epidermal Langerhans cells (LC) in specimens taken from involved areas and peripheral normally pigmented skin in vitiligo, idiopathic guttate hypomelanosis (IGH) and photoleukomelanoderma, principally by means of staining for ATPase.Ia antigen staining was used as supplementary identification method for LC.A decrease in the density of LC in peripheral normally pigmented skin was observed in 4 of 8 specimens from normoimmunological vitiligo, and in 4 of 5 specimens from dysimmunological vitiligo. A remarkable decrease in the density of LC in involved areas was observed in IGH. The differences in LC density between vitiligo and IGH were highly suggestive of possible differences in the pathogenesis of leukoderma. We also discussed some possible roles of LC in the pathogenesis of vitiligo.
CALMODULIN ACTIVITY IN THE PSORIATIC EPIDERMIS
Authors: Kusakabe Yoshiyuki , Iizuka Hajime , Matsuo Shinobu , Kajita Satoshi , Tsutsui Masato , Mizumoto Toshihiro , Ohkawara Akira , Yamauchi Takashi 
Published: April 2015

ABSTRACTRecent reports suggest there is a growth‐associated fluctuation of calmodulin content in various tissues. Since psoriatic epidermis is one of the most proliferative epidermal tissues, we studied the calmodulin content of psoriatic involved and uninvolved epidermis.By measuring activation of the calmodulin‐deficient phosphodiesterase purified from bovine brain, we assayed and compared the calmodulin activity in involved and uninvolved epidermis of seven psoriatic patients. Calmodulin purified from bovine brain was used as a standard. The mean calmodulin activity of the involved epidermis was 299 ± 149 (µg calmodulin activity/g.w.w. ±S.D.) or 9.6 ± 3.0 (µg calmodulin activity/mg protein). The mean calmodulin activity of the uninvolved epidermis was 251 ± 96 (µg calmodulin activity/g.w.w.) or 9.2 ± 2.9 (µg calmodulin activity/mg protein). These results indicate that psoriatic involved and uninvolved epidermis contains significant amounts of calmodulin, and that no marked differences exist in calmodulin content between these two areas.