bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence, Correlation and Clinical Outcome of Intra‐Procedural Stent Thrombosis in Patients Undergoing Primary Percutaneous Coronary Intervention for Acute Coronary Syndrome
Authors: XU YINGJIA , QU XINKAI , FANG WEIYI , CHEN HUI 
Published: April 2013

AbstractObjectivesTo assess the occurrence, correlation, and clinical outcome of intraprocedural stent thrombosis (IPST) in patients undergoing primary percutaneous coronary intervention (PCI) in the setting of acute coronary syndromes (ACSs).BackgroundStent thrombosis (ST), a rare complication of PCI, is more common in the setting of ACS. It is not known whether IPST carries the same prognosis as postprocedural ST.MethodsThis retrospective study comprised a review of 1,901 consecutive ACS patients who received primary PCI in our center from January 2006 to January 2011. IPST was defined as new, reappearing or increased thrombus within the deployed stent before the index PCI procedure was completed. All angiograms were independently reviewed frame by frame for the incidence of IPST. Patients with and without IPST were compared with respect to clinical characteristics, angiographic parameters, and major adverse cardiac events (MACEs) at 30 days and 1‐year follow‐up.ResultsOverall, there were 23 cases of IPST detected, thus, the prevalence of IPST was 1.2%. There were no significant differences in baseline clinical characteristics between the 2 groups. Patients with compared to those without IPST had significantly more bifurcation lesions involved, and more thrombus burden at baseline. IPST group compared to no IPST group had more MACEs on 30 days (26.1% vs. 8.7%, P = 0.01) and 1‐year follow‐up (30.4% vs. 14.4%, P = 0.02).ConclusionsIPST was a rare complication of PCI in the setting of ACS. It correlated with lesion morphology, presence of thrombus at baseline and was more likely to cause MACEs in 30 days and 1‐year follow‐up. (J Interven Cardiol 2013;26:215–220)
Taxonomic Characterization and Experimental Host Ranges of Four Newly Recorded Species ofAlternariafromJapan
Authors: Nishikawa Junji , Nakashima Chiharu 
Published: April 2013

AbstractAlternariafungi are important plant pathogens. Here, we identified three species new to theJapanese mycoflora:Alternaria celosiae,Alternaria crassaandAlternaria petroselini. We proposed a new name forA. celosiae(E.G.Simmons &Holcomb)Lawrence,Park &Pryor, a later homonym ofA. celosiae(Tassi)O.Săvul. To characterize these and a fourth morphological taxon,Alternaria alstroemeriae, which was recently added toJapan's mycoflora, an integrated species concept was tested. We determined the host range of each isolate using inoculation tests and analysed its phylogenetic position using sequences of the internal transcribed spacer rDNA. The pathogenicity of ourA. alstroemeriaeisolate was strictly limited toAlstroemeriasp. (Alstroemeriaceae), but the species was phylogenetically indistinguishable from other small‐sporedAlternaria.Alternaria celosiaeonCelosia argenteavar.plumosa(Amaranthaceae) was also pathogenic toAmaranthus tricolor, toAlternanthera paronychioidesand weakly toGomphrena globosa(all Amaranthaceae) and formed a clade with the formerNimbya celosiae.Alternaria crassaonDatura stramonium(Solanaceae) was also pathogenic toBrugmansia × candidaandCapsicum annuuminSolanaceae, but not to other confamilial plants; phylogenetically it belonged to a clade of large‐spored species with filamentous beaks. Morphological similarity, phylogenetic relationship and experimental host range suggested thatA. crassa,Alternaria capsiciandAlternaria daturicolawere conspecific.Alternaria petroselinionPetroselinum crispum(Apiaceae) was pathogenic to five species in the tribeApieae as well as representatives ofBupleureae,Coriandreae,Seliaeae andScandiceae inApiaceae. Both phylogeny and morphology suggested conspecificity betweenA. petroseliniandAlternaria selini.
Gastrointestinal Hemodynamics in Dogs with Nonfood Induced Atopic Dermatitis
Authors: Bruet V. , Brune J. , Pastor A. , Imparato L. , Roussel A. , Bourdeau P. , Desfontis J.C. 
Published: April 2013

BackgroundCanine atopic dermatitis can be a result of exposure to aeroallergens or trophallergens. Hemodynamic alterations occur in dogs with food hypersensitivity.Hypothesis/ObjectivesTo evaluate if hemodynamic alterations occur in dogs with NFICAD with lowered resistance to diastolic flow at fasting, after feeding, or both.AnimalsTen healthy dogs and 22 dogs with NFICAD were included from the hospital population.MethodsBlinded prospective study. Peak systolic velocity (PSV), end diastolic velocity (EDV), mean velocity (MV), pulsatility index (PI), resistive index (RI) and PSV/EDV ratio were measured at fasting for both arteries (cranial mesenteric artery [CMA], celiac artery [CA]) and at 40 minutes after feeding in CMA and at 60 minutes in CA. The results were analyzed statistically with a mixed model.ResultsThere was no difference detected between groups of dogs for any variable except EDV during fasting (P = .01).Conclusions and Clinical ImportanceThere is no decrease in resistance in NFICAD to diastolic flow. This observation could be explained by the absence intestinal inflammation in NFICAD.
Multicenter randomized prospective clinical evaluation of meloxicam administered via transmucosal oral spray in client‐owned dogs
Authors: Cozzi E. M. , Spensley M. S. 
Published: April 2013

The clinical safety and efficacy of a transmucosal oral spray (TMOS) formulation of meloxicam was evaluated for the control of pain and inflammation associated with osteoarthritis in dogs. A total of 280 client‐owned dogs were enrolled at fourteen veterinary clinics: there were 187 dogs in the meloxicamTMOSgroup and 93 in the placebo control group. Dogs received placebo or treatment spray once daily for twenty‐eight days. Improvement in signs of osteoarthritis was measured using client‐specific outcome measures (CSOM) made at days 14 and 28 and veterinary assessments of lameness and pain on palpation made at day 28. A significantly higher number of dogs in the meloxicamTMOSgroup were treatment successes at 28 days (72.6%) compared with the placebo group (46.9%), based onCSOMscores. TotalCSOMscores were significantly lower in the meloxicamTMOS‐treated group compared with the placebo group at both 14 and 28 days. Differences between treatment groups were not observed in veterinary assessments. Gastrointestinal effects of meloxicam were observed in some animals. Meloxicam TMOS was found to be safe and effective in dogs for the control of pain and inflammation associated with osteoarthritis.
Dehydrated egg white: An allergen source for improving efficacy and safety in the diagnosis and treatment for egg allergy
Authors: Escudero Carmelo , Sánchez‐García Silvia , Rodríguez del Río Pablo , Pastor‐Vargas Carlos , García‐Fernández Cristina , Pérez‐Rangel Inmaculada , Ramírez‐Jiménez Antonio , Ibáñez María Dolores 
Published: April 2013

AbstractBackgroundRaw and cooked eggs are used as allergens in oral food challenge (OFC). Raw egg is the best option, as it keeps proteins intact and retains their allergenicity, albeit microbiologically safe manipulation is difficult. Therefore, the use of dehydrated egg white (DEW) could improve the efficacy and safety profile of OFC. The aim of the study was to compare the allergenicity of DEW, a product that undergoes a double heat treatment (pasteurization and drying), with that of raw egg white (REW) and determine the efficacy of DEW in the diagnosis of egg allergy.MethodsWe conducted a prospective study of 40 egg‐allergic patients who visited our outpatient clinic. Each patient underwent OFC with DEW and REW to determine the correlation between the tests. DEW and REW extracts were analyzed using SDS‐PAGE. We compared the allergenicity of both extracts using IgE immunoblotting with a serum pool from patients with positive OFC results.ResultsTen patients (25%) had positive OFC results with both DEW and REW, and the doses that triggered an allergic reaction in each patient were similar (p > 0.05). All 30 patients (75%) with a negative OFC result with DEW also had negative OFC results with REW. SDS‐PAGE and IgE immunoblotting revealed that the protein composition and IgE‐binding capacity of both extracts were virtually identical.ConclusionsThis is the first time that it is shown that the allergenicity of commercially available DEW is equivalent to raw egg whites. In vivo and in vitro tests showed that processing of DEW does not affect the allergenicity of egg proteins. DEW is an effective and microbiologically safer source of allergen for the diagnosis of egg allergy. Furthermore, DEW can be used in egg oral immunotherapy.
The combined effects of family size and farm exposure on childhood hay fever and atopy
Authors: Genuneit Jon , Strachan David P. , Büchele Gisela , Weber Juliane , Loss Georg , Sozanska Barbara , Boznanski Andrzej , Horak Elisabeth , Heederik Dick , Braun‐Fahrländer Charlotte , von Mutius Erika ,   the GABRIELA study group
Published: April 2013

AbstractBackgroundExposure to farming environments and siblings is associated with reduced risks of childhood hay fever and atopy. We explored the independence and interaction of these protective effects in the GABRIELA study.MethodsQuestionnaire surveys on farming, asthma, and allergies were conducted in four central European areas among 79,888 6–12‐yr‐old children. Aeroallergen‐specific serum IgE was measured in a stratified sample of 8,023 children. Multiple logistic regression was used to compare gradients in allergy prevalence by sibship size across three categories of exposure to farming environments.ResultsThe prevalence of hay fever ranged from 2% (95% confidence interval 1.6%; 2.7%) among farmers' children with more than two siblings to 12% (11.2%; 13.0%) among children with no farm exposure and no siblings. Farming families were larger on average. More siblings and exposure to farming environments independently conferred protection from hay fever and atopy. There was no substantial effect modification between family size and exposure to farming environments. The odds ratios for hay fever per additional sibling were 0.79 among unexposed non‐farm children, 0.77 among farm‐exposed non‐farm children, and 0.72 among children from farming families (2df interaction test: p = 0.41).ConclusionThe inverse association of exposure to farming environments with hay fever is found in all sizes of family, with no substantial tendency to saturation or synergism. This suggests that different biological mechanisms may underlie these two protective factors. Combinations of a large family and exposure to farming environments markedly reduce the prevalence of hay fever and indicate the strength of its environmental determinants.
Characterization of the sensitization profile to lupin in peanut‐allergic children and assessment of cross‐reactivity risk
Authors: Ballabio Cinzia , Peñas Elena , Uberti Francesca , Fiocchi Alessandro , Duranti Marcello , Magni Chiara , Restani Patrizia 
Published: April 2013

AbstractBackgroundCase reports of allergy to lupin, due to primary sensitization or cross‐reactions with other legumes, are increasing as a consequence of the augmented use of lupin flour in bakery, pasta formulations and other food items. The main allergens that have been associated with the sensitization to lupin are α‐ and β‐conglutins and, to a lesser extent, γ‐ and δ‐conglutin, but no conclusive data are available so far. The aim of this study was to characterize the sensitization pattern to lupin in a group of 12 Italian children allergic to peanut and identify the specific lupin proteins involved in the cross‐reactivity with peanut.MethodsThe immunochemical cross‐reactivity among peanut and lupin was evaluated by both in vitro immunoblotting and in vivo fresh food skin prick test (FFSPT).ResultsThe results showed that β‐conglutin was recognized by cutaneous IgEs from 7/12 peanut‐allergic children in FFSPT and serum IgEs from 5/12 in immunoblotting, while 4/12 and 8/12 patients tested positive to γ‐conglutin in FFSPT and immunoblotting, respectively. No significant immunoreactive responses were observed to α‐ and δ‐conglutins under non‐reducing conditions, but they were bound in FFSPT by the sera of 5/12 and 3/12 patients, respectively.ConclusionIn this group of allergic children, β‐conglutin has been identified as the major lupin allergen involved both in vitro and in vivo cross‐reactivity with peanut proteins. The role of γ‐conglutin in the cross‐reactivity between lupin and peanut proteins was also relevant and clear, despite the observed unspecificity of the immunoblotting responses.
Development of EST‐SSR markers for diversity and breeding studies in opium poppy
Authors: Şelale Hatice , Çelik Ibrahim , Gültekin Visam , Allmer Jens , Doğanlar Sami , Frary Anne 
Published: April 2013

AbstractAll publicly available opium poppy expressed sequence tag (EST) sequences, totalling 20 885, were assembled into unigenes and examined for simple sequence repeats (SSRs). Nearly 19% of the 14 957 unigenes contained SSRs with 4% harbouring more than one SSR. Average density of the SSRs was 1 SSR per 3.6 kb of non‐redundant EST sequence. Trinucleotide SSRs were most frequently identified (39%), and many of the most prevalent motifs were AT‐rich. Flanking primers were designed for 86% of the SSRs and 67 primer pairs were tested on 37 opium poppy accessions and seven related species. All markers were transferable to the related species. Polymorphism information content (PIC) values for the markers were intermediate for comparisons within opium poppy (average of 0.27) and slightly higher for comparisons across species (average of 0.29). The markers were found to be useful for diversity analysis as they successfully distinguished among Turkish opium poppy accessions and land races.
Effects of intranasal oxytocin on pupil dilation indicate increased salience of socioaffective stimuli
Authors: Prehn Kristin , Kazzer Philipp , Lischke Alexander , Heinrichs Markus , Herpertz Sabine C. , Domes Gregor 
Published: April 2013

AbstractTo investigate the mechanisms by which oxytocin improves socioaffective processing, we measured behavioral and pupillometric data during a dynamic facial emotion recognition task. In a double‐blind between‐subjects design, 47 men received either 24 IU intranasal oxytocin (OXT) or a placebo (PLC). Participants in the OXT group recognized all facial expressions at lower intensity levels than did participants in the PLC group. Improved performance was accompanied by increased task‐related pupil dilation, indicating an increased recruitment of attentional resources. We also found increased pupil dilation during the processing of female compared with male faces. This gender‐specific stimulus effect diminished in the OXT group, in which pupil size specifically increased for male faces. Results suggest that improved emotion recognition after OXT treatment might be due to an intensified processing of stimuli that usually do not recruit much attention.
Challenges to the global control of tuberculosis
Authors: Chiang Chen‐Yuan , Van Weezenbeek Catharina , Mori Toru , Enarson Donald A. 
Published: April 2013

AbstractDiagnosis and treatment of tuberculosis (TB) will likely navigate a historical turning point in the 2010s with a new management paradigm emerging. However, global control of TB remains a formidable challenge for the decades to come. The estimated case detection rate of TB globally was 66%, and there were 310 000 estimated multidrug‐resistant TB (MDR‐TB) cases among the 6.2 million TB patients notified in 2011. Although new tools are being introduced for the diagnosis of MDR‐TB, there are operational and cost issues related to their use that require urgent attention, so that the poor and vulnerable can benefit. World Health Organization (WHO) estimated that globally, 3.7% of new cases and 20% of previously treated cases have MDR‐TB. However, the scale‐up of programmatic management of drug‐resistant TB is slow, with only 60 000 MDR‐TB cases notified to WHO in 2011. The overall proportion of treatment success of MDR‐TB notified globally in 2009 was 48%, far below the global target of 75% success rate. Although new tools and drugs have the potential to significantly improve both case detection and treatment outcome, adequate health systems and human resources are needed for rapid uptake and proper implementation to have the impact required to eliminate TB. Hence, the global TB community should broaden its scope, seek intersectoral collaboration and advocate for cost reduction of new tools, while ensuring that the basics of TB control are implemented to reduce the TB burden through the current ‘prevention through case management’ paradigm.
Social provision and loneliness among older people suffering from chronic physical illness. A mixed‐methods approach
Authors: Kvaal Kari , Halding Anne‐Grethe , Kvigne Kari 
Published: April 2013

AimsTo describe and compare the perceived social provision for a group reporting never feeling lonely with that of a group reporting feeling lonely and to explore the meaning of loneliness.SubjectsParticipants (N = 101) were recruited from geriatric wards. Inclusion criteria were as follows: aged 65 years or more, the absence of dementia, one or more chronic physical disorders and plans to be discharged from the hospital to their home. The mean age was 81.3 years (range: 65–96 years), 68% were women, and 66% lived alone.MeasuresAssessments of social provisions and loneliness were collected by a subjective report using the Social Provision Scale (SPS), and the Montgomery–Aasberg Depression Rating Scale (MADRS) was used to assess depression. The participants were also asked whether they felt lonely and were then asked to describe the meaning of loneliness if they had indicated feeling lonely. Narratives were then condensed by the participants into short sentences.ResultsSeventy‐five per cent of the participants reported feeling lonely, of these 54% were living alone, and 18% identified with depression. Three subscales of SPS scores were significantly lower in the lonely group: attachment (p < 0.001), a sense of reliable alliance (p = 0.001) and the obtaining of guidance (p = 0.01). The overall view of the experience of loneliness was dominated by emptiness and negative emotions. The following themes were identified: Emotions were dominated by sadness, anxiety and restlessness, anger and guilt. Relationships were dominated by being left alone, being confined and feeling useless. Existential dimensions were characterised by emptiness, endless boredom, isolation and the potential for change.ConclusionThe study shows that loneliness is prevalent among older people suffering from chronic physical illness and confirms the complexity of the concept. A mixed‐methods design contributed to nuanced and detailed information about the meaning of loneliness.
Factors affecting red blood cell storage age at the time of transfusion
Authors: Dzik Walter H. , Beckman Neil , Murphy Michael F. , Delaney Meghan , Flanagan Peter , Fung Mark , Germain Marc , Haspel Richard L. , Lozano Miguel , Sacher Ronald , Szczepiorkowski Zbigniew , Wendel Silvano 
Published: April 2013

BackgroundClinical trials are investigating the potential benefit resulting from a reduced maximum storage interval for red blood cells (RBCs). The key drivers that determine RBC age at the time of issue vary among individual hospitals. Although progressive reduction in the maximum storage period of RBCs would be expected to result in smaller hospital inventories and reduced blood availability, the magnitude of the effect is unknown.Study Design and MethodsData on current hospital blood inventories were collected from 11 hospitals and three blood centers in five nations. A general predictive model for the age of RBCs at the time of issue was developed based on considerations of demand for RBCs in the hospital.ResultsAge of RBCs at issue is sensitive to the following factors: ABO group, storage age at the time of receipt by the hospital, the restock interval, inventory reserve, mean demand, and variation in demand.ConclusionsA simple model, based on hospital demand, may serve as the basis for examining factors affecting the storage age of RBCs in hospital inventories. The model suggests that the age of RBCs at the time of their issue to the patient depends on factors external to the hospital transfusion service. Any substantial change in the expiration date of stored RBCs will need to address the broad variation in demand for RBCs while attempting to balance considerations of availability and blood wastage.
Next‐generation sequencing: proof of concept for antenatal prediction of the fetal Kell blood group phenotype from cell‐free fetal DNA in maternal plasma
Authors: Rieneck Klaus , Bak Mads , Jønson Lars , Clausen Frederik Banch , Krog Grethe Risum , Tommerup Niels , Nielsen Leif Kofoed , Hedegaard Morten , Dziegiel Morten Hanefeld 
Published: April 2013

BackgroundMaternal immunization against KEL1 of the Kell blood group system can have serious adverse consequences for the fetus as well as the newborn baby. Therefore, it is important to determine the phenotype of the fetus to predict whether it is at risk. We present data that show the feasibility of predicting the fetal KEL1 phenotype using next‐generation sequencing (NGS) technology.Study Design and MethodsThe KEL1/2 single‐nucleotide polymorphism was polymerase chain reaction (PCR) amplified with one adjoining base, and the PCR product was sequenced using a genome analyzer (GAIIx, Illumina); several millions of PCR sequences were analyzed.ResultsThe results demonstrated the feasibility of diagnosing the fetal KEL1 or KEL2 blood group from cell‐free DNA purified from maternal plasma.ConclusionThis method requires only one primer pair, and the large amount of sequence information obtained allows well for statistical analysis of the data. This general approach can be integrated into current laboratory practice and has numerous applications. Besides DNA‐based predictions of blood group phenotypes, platelet phenotypes, or sickle cell anemia, and the determination of zygosity, various conditions of chimerism could also be examined using this approach. To our knowledge, this is the first report focused on antenatal blood group determination using NGS.