bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fluorescence Quenching by Intercalation of a Pyrene Group Tethered to an N4‐modified Cytosine in Duplex DNA
Authors: Avirah Rekha R. , Schuster Gary B. 
Published: September 2012

AbstractA series of duplex DNA oligomers was prepared that contain a pyrene chromophore linked by a trimethylene chain (‐(CH2)3‐) to N4 of a cytosine. The pyrene group stabilizes the DNA as evidenced by an increase in melting temperature. The absorption spectrum of the linked pyrene chromophore shows a temperature‐dependent shift and there is also a strong induced circular dichroism spectrum attributed to the pyrene group. The fluorescence of the pyrene chromophore is strongly quenched at room temperature by linkage to the DNA, but it increases above the melting temperature. We attribute these observations to intramolecular intercalation of the pyrene group at a base pair adjacent to its linkage site at cytosine.
Search and Validation of Acidity References in Sensory Evaluation of Pepper
Authors: Torre Paloma , Arana Inés , Ortigosa María , Ibáñez Francisco C. 
Published: September 2012

AbstractThe accreditation of sensory methods for quality control of specific products requires the fulfillment of interlaboratory tests. These tests are one of the requirements to guarantee the technical competence of the laboratories in an independent way. In order to compare the laboratory results, it is convenient to use references in judge training. This investigation shows the efficiency of the references for evaluating the intensity of the acidity during sensory quality control of peppers with protected designation of origin (PDO). Such references are prepared from a vegetable food matrix. Three references have been obtained for the acidity intensity and they have been tested with three sensory panels from different laboratories. The samples used belonged to a PDO. It also compared the use of two response scales in a trained panel. The results show a high correlation between both response scales and it can be considered as a preliminary study of the development of acidity references to be used for improvement of sensory quality control procedures of some type of pepper.Practical ApplicationsThis study provides the guidelines for development of acid references in a vegetable food. These references are relevant to minimize the variability in sensory evaluation for quality control of protected designation of origin.The use of a reference standard for intensity is helpful in intercomparison testing studies.
Detection and Identification of Two Phytoplasmas (16SrIII‐B and 16SrXII‐A) From Alfalfa (Medicago sativa) in Serbia
Authors: Starović Mira , Kuzmanović Slobodan , Gavrilović Veljko , Aleksić Goran , Popović Tatjana , Stojanović Saša , Jošić Dragana 
Published: September 2012

AbstractPlants of alfalfa (Medicago sativa) exhibiting general stunting, proliferation and phyllody associated with leaf yellowing and reddening were observed in three localities of Central Serbia. Phytoplasma strains belonging to 16SrIII‐B and 16SrXII‐A groups were detected and identified by RFLP and sequence analysis of 16S rDNA. Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the TufAY‐b‐type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16SrIII‐B and 16SrXII‐A phytoplasma groups affecting alfalfa in Serbia.
Detection and identification of ‘Candidatus Phytoplasma asteris’ in Brassica rapa subsp. pekinensis in Poland
Authors: Kamińska Maria , Berniak Hanna , Kamiński Piotr 
Published: September 2012

AbstractSevere growth abnormalities, including leaf yellowing, sprout proliferation and flower virescence and phyllody, were found on Brassica rapa subsp. pekinensis plants in Poland. The presence of phytoplasma in naturally infected plants was demonstrated by polymerase chain reaction assay employing phytoplasma universal P1/P7 followed by R16F2n/R16R2 primer pairs. The detected phytoplasma was identified using restriction fragment length polymorphism analysis (RFLP) of the 16S rRNA gene fragment with AluI, HhaI, MseI and RsaI endonucleases. After enzymatic digestion, all tested samples showed restriction pattern similar to that of ‘Candidatus phytoplasma asteris’. Nested PCR‐amplified products, obtained with primers R16F2n/R16R2, were sequenced. Sequences of the 16S rDNA gene fragment of analysed phytoplasma isolates were nearly identical. They revealed high nucleotide sequence identity (>98%) with corresponding sequences of other phytoplasma isolates from subgroup 16SrI‐B, and they were classified as members of ‘Candidatus phytoplasma asteris’. This is the first report of the natural occurrence of phytoplasma‐associated disease in plants of Chinese cabbage.
Treatment patterns in major depressive disorder after an inadequate response to first-line antidepressant treatment
Authors: Garcia-Toro Mauro , Medina Esteban , Galan Jaime L , Gonzalez Miguel A , Maurino Jorge 
Published: September 2012

Abstract

Background
The aim of the study was to determine the most common pharmacological strategies used in the management of major depressive disorder (MDD) after an inadequate response to first-line antidepressant treatment in clinical practice.

Methods
Multicenter, non-interventional study in adult outpatients with a DSM-IV-TR diagnosis of MDD and inadequate response to first-line antidepressant medication. Multiple logistic regression analyses were performed to identify independent factors associated with the adoption of a specific second-line strategy.

Results
A total of 273 patients were analyzed (mean age: 46.8 years, 67.8% female). Baseline mean Montgomery-Asberg Depression Rating Scale total score was 32.1 (95%CI 31.2-32.9). The most common strategies were: switching antidepressant medication (39.6%), augmentation (18.8%), and combination therapy (17.9%). Atypical antipsychotic drugs were the most commonly used agent for augmenting antidepressant effect. The presence of psychotic symptoms and the number of previous major depressive episodes were associated with the adoption of augmenting strategy (OR = 3.2 and 1.2, respectively).

Conclusion
The switch to another antidepressant agent was the most common second-line therapeutic approach. Psychiatrists chose augmentation based on a worse patients’ clinical profile (number of previous episodes and presence of psychotic symptoms).
Oxygen Liberation from Stabilized Zirconia upon Changing DC Polarization Condition
Authors: Kurita Noriaki , Kobayashi Kenji , Heda Yoshinori , Fukatsu Norihiko 
Published: September 2012

AbstractThe compositional change of stabilized zirconia upon the change of oxygen chemical potential was studied by applying DC-polarization method to a galvanic cell using stabilized zirconia as the electrolyte. The flow of excess charge observed upon the change of the DC-polarization condition was found due to the oxygen liberation from the bulk of the electrolyte. The total amount of liberated oxygen determined by the excess charge was analyzed in terms of the compositional change of the electrolyte due to the change of oxygen chemical potential profile in the electrolyte. The calculated values based on the increase or decrease of the electronic defects could not account for the experimental values. However, the ones based on the reduction of impurity oxides were in good agreement with the experimental values. Considerable amount of oxygen can be released from or absorbed into the zirconia electrolyte by the redox reaction of impurity oxide. It is noteworthy that this phenomenon may cause serious errors to the transport properties of electronic defect determined with the use of the relaxation processes.
Municipal Archaeology Programs and the Creation of Community Amenities
Authors: Appler Douglas R. 
Published: September 2012

Abstract
This paper explores how the municipal archaeology programs found in Alexandria, Virginia; St. Augustine, Florida; and Phoenix, Arizona have played a prominent role in developing unique, place-based amenities that integrate local history with other community needs. These cities are unusual in that they maintain archaeologists on city staff and that those archaeologists have used their positions to develop local environments that are extremely supportive of public engagement with history. Using interviews as well as archival and documentary sources, this paper demonstrates how the public's resulting familiarity with archaeology has allowed the interpretation of local history to take a variety of unexpected forms, including public and private open spaces, urban walking and cycling trails, museums, and public art, among many others.
Improving Delivery of EPSDT Well-Child Care at Acute Visits in an Academic Pediatric Practice
Authors: Patterson Barron L. , Gregg William M. , Biggers Cynthia , Barkin Shari 
Published: September 2012

BACKGROUND AND OBJECTIVE:Many patients with Medicaid do not receive timely, comprehensive well-child care through the Early and Periodic Screening, Diagnosis, and Treatment (EPSDT) program. Missed opportunities for EPSDT well-child check-ups (WCCs) at acute visits contribute to this problem. The authors sought to reduce missed opportunities for WCCs at acute visits for patients overdue for those services.METHODS:A quality improvement team developed key drivers and used a people-process-technology framework to devise 3 interventions: (1) an electronic indicator based on novel definitions of EPSDT status (up-to-date, due, overdue, no EPSDT), (2) a standardized scheduling process for acute visits based on EPSDT status, and (3) a dedicated nurse practitioner to provide WCCs at acute visits. Data were collected for 1 year after full implementation.RESULTS:At baseline, 10.3 acute visits per month were converted to WCCs. After intervention, 86.7 acute visits per month were converted. Of 13 801 acute visits during the project, 31.2% were not up-to-date. Of those overdue for WCCs, 51.4% (n = 552) were converted to a WCC in addition to the acute visit. Including all patients who were not up-to-date, a total of 1047 acute visits (7.6% of all acute visits) were converted to comprehensive WCCs. Deferring needed WCCs at acute visits resulted in few patients who scheduled or completed future WCC visits.CONCLUSIONS:Implementation of interventions focused on people-process-technology significantly increased WCCs at acute visits within a feasible and practical model that may be replicated at other academic general pediatrics practices.
On-line Certification for All: The PINVOX Algorithm
Authors: Canessa E , Fonda C , Zennaro M 
Published: September 2012

A protoype algorithm: PINVOX (â??Personal
Identification Number by Voice") for on-line certification is
introduced to guarantee that scholars have followed, i.e.,
listened and watched, a complete recorded lecture with the
option of earning a certificate or diploma of completion
after remotely attending courses. It is based on the injection
of unique, randomly selected and pre-recorded integer
numbers (or single letters or words) within the audio trace
of a video stream at places where silence is automatically
detected. The certificate of completion or â??virtual
attendanceâ? is generated on-the-fly after the successful
identification of the embedded PINVOX code by a video
viewer student.
Kalinga Textile Design Development and Evaluation
Authors: Odiem Marymina P. , Bawer Ma. Cyrila , Bagtang Eduardo T. 
Published: September 2012

Weaving is one of the major economic activities among women in the province of Kalinga, Philippines. The study developed and evaluated the Kalinga weave designs. The researchers conceptualized modified weave designs. The weave designs were taught to 15 weavers through five day training. Specifically, the project upgrades the weaving skills and techniques of weavers in terms of designs, color combination, and workmanship. The results showed that the making of bags, men’s attires, ladies suits, accessories, and for accents. Fifteen woven items produced different designs. The woven products were mixed with some of the existing designs and evaluated. Results of the evaluation revealed that in terms of color combination, design, workmanship, and best utilization of the products, the assessment of the evaluators did not significantly differ. The modified cloth designs can compete with the traditional designs in terms of color combinations, designs and workmanship. Keywords - weave design development, evaluation, improvement
Disease‐specific databases: Why we need them and some recommendations from the Human Variome Project Meeting, May 28, 2011
Authors: Howard Heather J. , Beaudet Arthur , Gil‐da‐Silva Lopes Vera , Lyne Mike , Suthers Graeme , Van den Akker Peter , Wertheim‐Tysarowska Katarzyna , Willems Patrick , Macrae Finlay 
Published: September 2012

AbstractThe need for Locus‐Specific Databases, with disease‐specific experts and curators, is an essential ingredient in a process to enable the benefits of the advances in sequencing and mutational analysis to be realized across the genome. Next generation sequencing provides both astounding opportunities and challenges, especially for genetic counsellors. An approach coordinated at a genome wide, international level, supported by well‐organized disease‐specific respected organizations is a model most likely to be successful, but committed resourceful professionals working in local poorly resourced environments can make valuable contributions that can grow. Bioinformatic tools to sift and integrate multiple domains of information are being developed, and play a major part in meeting the challenges. Regulation of providers, including a requirement for them to submit mutational information to central databases, also should assist to reach the goals needed to realize the opportunities. There is also a need to agree on governance of Locus‐Specific Databases (LSDBs) at an international level, and for adequate international funding to support this need, to ensure humanity reaps the benefits of the current molecular genetic revolution. The Human Variome Project offers this, working also with the other major initiatives with similar objectives. This report concludes with Recommendations for the Human Variome Project stemming from the presentations and discussions at the meeting. © 2012 Wiley Periodicals, Inc.
Death rates in the U.S. due to Krabbe disease and related leukodystrophy and lysosomal storage diseases
Authors: Barczykowski Amy L. , Foss Alexander H. , Duffner Patricia K. , Yan Li , Carter Randy L. 
Published: September 2012

AbstractLeukodystrophies (LD) and lysosomal storage disorders (LSD) have generated increased interest recently as targets for newborn screening programs. Accurate epidemiological benchmarks are needed in the U.S. Age‐specific mortality rates were estimated for Krabbe disease (KD) and nine related disorders. U.S. mortality records with E75.2 cause of death code during 1999–2004 were collected from 11 open record states. All E75.2 deaths in the United States were distributed into specific disease type based on proportions observed in these states. Yearly population sizes were obtained from the CDC and averaged. Mortality rates (per million individuals per year) by age group for the specific diseases were (for <5 or ≥5 years): Pelizaeus‐Merzbacher (0.037/0.033); sudanophilic leukodystrophy (SLD) (0.037/0.004); Canavan (0.037/0.011), Alexander (0.147/0.022); Krabbe (0.994/0.007); metachromatic leukodystrophy (0.331/0.135); Fabry (0.000/0.124); Gaucher (0.221/0.073); Niemann–Pick (NP) (0.442/0.088); multiple sulfatase (0.000/0.004). This is the first report of mortality rates for the LD/LSD diseases in the U.S. Approximated birth prevalence rate for the early infantile Krabbe phenotype (onset 0–6 months) was based on the <5 year old mortality rate of one early infantile case per 244,000 births, which matches the 1 in 250,000 observed in the NYS newborn screening program as of 2011. It should be noted however that the NYS calculation refers only to the early infantile phenotype and does not include the majority of babies identified in the program with low GALC and two mutations who have remained clinically normal. It is presumed that most, if not all, will develop later onset forms of the disease, but this is by no means certain. © 2012 Wiley Periodicals, Inc.
Decomposition of 2‐chlorophenol, 4‐chlorophenol and 2,4,6‐trichlorophenol by catalytic oxidation over cobalt and nickel impregnated SBA‐15
Authors: Mayani Suranjana V. , Mayani Vishal J. , Kim Sang Wook 
Published: September 2012

AbstractThis work describes the use of Co(II) and Ni(II) impregnated SBA‐15 as catalysts for the oxidative degradation of a few persistent chlorinated phenols in an aqueous medium: 2‐chlorophenol (2‐CP), 4‐chlorophenol (4‐CP) and 2,4,6‐trichlorophenol (2,4,6‐TCP). The catalysts were characterised in terms of their crystallographic features, surface topography, functional groups, thermal stability, etc. The oxidation reactions were carried out using the reaction time, concentration of chlorophenol, amount of catalyst and pH of the reaction mixture as the process variables with or without hydrogen peroxide as the chemical oxidising agent. The conversion achieved with Co/SBA‐15 for 2‐CP, 4‐CP and 2,4,6‐TCP was respectively 84.7%, 78.4% and 64.8% with H2O2 and 86.3%, 80.2% and 70.3% in the absence of H2O2. The conversion with Ni/SBA‐15 also at 353 K for 2‐CP, 4‐CP and 2,4,6‐TCP was, respectively, 82.3%, 81.9% and 64.0% at 5 h with H2O2 and 89.5%, 82.9% and 65.6% without H2O2. The reactions followed pseudo‐first‐order kinetics. The leachability study indicated that the catalysts release very little Co and Ni to water. Therefore, the possibility of water contamination through metal leaching was almost negligible. Oxidative degradation was confirmed by measuring the total organic carbon. © 2012 Canadian Society for Chemical Engineering
A population‐based study of metastatic colorectal cancer in individuals aged ≥80 years
Authors: Kumar Rajiv , Jain Kunal , Beeke Carol , Price Timothy J. , Townsend Amanda R. , Padbury Rob , Roder David , Young Graeme P. , Richards Alison , Karapetis Christos S. 
Published: September 2012

AbstractBACKGROUND.Life expectancy is increasing, and more patients are presenting with cancer at an advanced age (≥80 years). Optimal management for this group of patients has not been well defined.METHODS.The South Australian Clinical Registry for Metastatic Colorectal Cancer (mCRC) collects data on all patients diagnosed since February 2006 in South Australia. The authors examined cancer characteristics, treatments administered, and outcomes for patients aged ≥80 years compared with patients aged <80 years.RESULTSData from 2314 patients were evaluable, and 29.2% of these patients were aged ≥80 years. The majority had moderately differentiated tumors. Poorly differentiated tumors were reported in fewer patients aged ≥80 years (20.1% vs 26.1%; P < .005). Overall, 28.1% of patients aged ≥80 years received chemotherapy, and 74.2% received single‐agent fluoropyrimidines as first‐line treatment. By comparison, 68.2% of patients aged <80 years received chemotherapy, 74.3% received combination chemotherapy, and 25.7% received single‐agent fluoropyrimidine as first‐line treatment. No treatment was received by 38.2% of patients aged ≥80 years compared with 11.4% of those aged <80 years. Participation in clinical trials was lower in patients aged ≥80 years (2% vs 13%). The median survival was worse for patients aged ≥80 years (8.2 months vs 19.2 months; P < .001), and the median survival of patients who received chemotherapy was 19.0 months for those aged ≥80 years and 22.3 months for those aged <80 years (P = .139). Patients who did not receive treatment had a poor median survival regardless of age (2.6 months for patients aged ≥80 years vs 2.7 months for patients aged <80 years).CONCLUSIONS.Patients aged ≥80 years were less likely to receive intervention for their metastatic colorectal cancer and had poorer survival. The survival of selected patients aged ≥80 years who received chemotherapy was similar to the survival of those aged <80 years despite the receipt of single‐agent therapy. Patients aged ≥80 years with metastatic colorectal cancer are less likely to receive intervention for their disease and have poorer survival. Survival for selected patients aged ≥80 years who receive chemotherapy is similar to the survival of patients aged <80 years despite the receipt of single‐agent therapy. Cancer 2013. © 2012 American Cancer Society.
Links between psychopathological symptoms and disordered eating behaviors in overweight/obese youths
Authors: Pauli‐Pott Ursula , Becker Katja , Albayrak Özgür , Hebebrand Johannes , Pott Wilfried 
Published: September 2012

AbstractObjective:Among overweight and obese youths, rates of depression, anxiety disorders, attention‐deficit/hyperactivity disorder (ADHD), and oppositional defiant disorder (ODD) are elevated. We analyze whether these emotional and behavioral problems are associated with specifically disordered eating pattern.Method:Participants in the study were 128 overweight and obese children/adolescents (BMI: m = 29.3, s = 4.5; BMI‐SDS: m = 2.5, s = 0.4) between 8 and 15 years. Structured psychiatric assessments were conducted adopting a multimethod, multiinformant approach.Results:Children/adolescents with ODD symptoms showed increased eating in response to external cues and binge eating. ADHD symptoms were not associated with disordered eating behaviors. Children/adolescents with symptoms of depression and anxiety showed emotional and binge eating. In particular, overweight girls with symptoms of depression showed restrained eating.Discussion:Our results point to specific eating problems in overweight/obese children with ODD and depression/anxiety symptoms. The findings could help to tailor interventions to optimally meet the specific needs of overweight children with emotional and behavioral problems. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2013)
Immobilized Catalysts for Hydroformylation Reactions: A Versatile Tool for Aldehyde Synthesis
Authors: Neves Ângela C. B. , Calvete Mário J. F. , Pinho e Melo Teresa M. V. D. , Pereira Mariette M. 
Published: September 2012

AbstractOlefin hydroformylation is a widely used process, both on large scales and in fine chemistry, with catalyst reuse still being one of the main objectives. In recent years, developments in the immobilization of catalysts on solid supports that enable their recovery and reuse when applied in catalytic hydroformylations of aromatic and aliphatic olefins have resulted in a boost in the field of heterogeneous catalysis. This review presents the most relevant results reported over the last five years with regard to the development and use of supported catalysts in mesoporous materials, hydrotalcite, carbon materials, and nanoparticles for catalytic hydroformylation of olefins. A critical analysis of the results, especially in the context of reusability and leaching issues, is presented.