bluuebunny/crossref_metadata_2025_split huggingface dataset:
Emergency exsanguination of working equids
Authors: Cousquer Glen 
Published: February 2015

THIS series gives readers the opportunity to consider and contribute to discussion of some of the ethical dilemmas that can arise in veterinary practice. Each month, a case scenario is presented, followed by discussion of some of the issues involved.In addition, a possible way forward is suggested; however, there is rarely a cut‐and‐dried answer in such cases, and readers may wish to suggest an alternative approach. This month's dilemma, ‘Emergency exsanguination of working equids’, was submitted and is discussed by Glen Cousquer. Readers with comments to contribute are invited to send them as soon as possible, so that they can be considered for publication in the next issue. Discussion of the dilemma ‘Value and treatment’, which was published in the January issue of In Practice, appears on page 103.The series is being coordinated by Siobhan Mullan, of the University of Bristol. It is hoped it will provide a framework that will help practices find solutions when facing similar dilemmas.
Vertex criticality with respect to isolate domination
Authors: Sahul Hamid I. , Balamurugan S. 
Published: February 2015

A set S of vertices of a graph G is called a dominating set of G if every vertex in V(G) - S is adjacent to a vertex in S. A dominating set S such that the subgraph 〈S〉 induced by S has at least one isolated vertex is called an isolate dominating set. The minimum cardinality of an isolate dominating set is called the isolate domination number and is denoted by γ0(G). This concept was introduced in [I. Sahul Hamid and S. Balamurugan, Isolate domination in graphs (Communicated)] and further studied in [I. Sahul Hamid and S. Balamurugan, Extended chain of domination parameters in graphs, ISRN Combin. 2013 (2013), Article ID: 792743, 4 pp.; Isolate domination and maximum degree, Bull. Int. Math. Virtual Inst. 3 (2013) 127–133; Isolate domination in unicyclic graphs, Int. J. Math. Soft Comput. 3(3) (2013) 79–83]. This paper studies the effect of the removal of a vertex upon the isolate domination number.
Integrated Genomic Profiling, Therapy Response, and Survival in Adult Acute Myelogenous Leukemia
Authors: Parkin Brian , Ouillette Peter , Yildiz Mehmet , Saiya-Cork Kamlai , Shedden Kerby , Malek Sami N. 
Published: February 2015

Abstract
Purpose: Recurrent gene mutations, chromosomal translocations, and acquired genomic copy number aberrations (aCNA) have been variously associated with acute myelogenous leukemia (AML) patient outcome. However, knowledge of the co-occurrence of such lesions and the relative influence of different types of genomic alterations on clinical outcomes in AML is still evolving.
Experimental Design: We performed SNP 6.0 array-based genomic profiling of aCNA/copy neutral loss-of-heterozygosity (cnLOH) along with sequence analysis of 13 commonly mutated genes on purified leukemic blast DNA from 156 prospectively enrolled non-FAB-M3 AML patients across the clinical spectrum of de novo, secondary, and therapy-related AML.
Results: TP53 and RUNX1 mutations are strongly associated with the presence of SNP-A–based aCNA/cnLOH, while FLT3 and NPM1 mutations are strongly associated with the absence of aCNA/cnLOH. The presence of mutations in RUNX1, ASXL1, and TP53, elevated SNP-A–based genomic complexity, and specific recurrent aCNAs predicted failure to achieve a complete response to induction chemotherapy. The presence of ≥1 aCNA/cnLOH and higher thresholds predicted for poor long-term survival irrespective of TP53 status, and the presence of ≥1 aCNA/cnLOH added negative prognostic information to knowledge of mutations in TET2, IDH1, NPM1, DNMT3A, and RUNX1. Results of multivariate analyses support a dominant role for TP53 mutations and a role for elevated genomic complexity as predictors of short survival in AML.
Conclusions: Integrated genomic profiling of a clinically relevant adult AML cohort identified genomic aberrations most associated with SNP-A–based genomic complexity, resistance to intensive induction therapies, and shortened overall survival. Identifying SNP-A–based lesions adds prognostic value to the status of several recurrently mutated genes. Clin Cancer Res; 21(9); 2045–56. ©2015 AACR.
The Histone Demethylase Jumonji Coordinates Cellular Senescence Including Secretion of Neural Stem Cell–Attracting Cytokines
Authors: Perrigue Patrick M. , Silva Michael E. , Warden Charles D. , Feng Nathan L. , Reid Michael A. , Mota Daniel J. , Joseph Lauren P. , Tian Yangzi Isabel , Glackin Carlotta A. , Gutova Margarita , Najbauer Joseph , Aboody Karen S. , Barish Michael E. 
Published: February 2015

AbstractJumonji domain-containing protein 3 (JMJD3/KDM6B) demethylates lysine 27 on histone H3 (H3K27me3), a repressive epigenetic mark controlling chromatin organization and cellular senescence. To better understand the functional consequences of JMJD3 its expression was investigated in brain tumor cells. Querying patient expression profile databases confirmed JMJD3 overexpression in high-grade glioma. Immunochemical staining of two glioma cell lines, U251 and U87, indicated intrinsic differences in JMJD3 expression levels that were reflected in changes in cell phenotype and variations associated with cellular senescence, including senescence-associated β-galactosidase (SA-β-gal) activity and the senescence-associated secretory phenotype (SASP). Overexpressing wild-type JMJD3 (JMJD3wt) activated SASP-associated genes, enhanced SA-β-gal activity, and induced nuclear blebbing. Conversely, overexpression of a catalytically inactive dominant negative mutant JMJD3 (JMJD3mut) increased proliferation. In addition, a large number of transcripts were identified by RNA-seq as altered in JMJD3 overexpressing cells, including cancer- and inflammation-related transcripts as defined by Ingenuity Pathway Analysis. These results suggest that expression of the SASP in the context of cancer undermines normal tissue homeostasis and contributes to tumorigenesis and tumor progression. These studies are therapeutically relevant because inflammatory cytokines have been linked to homing of neural stem cells and other stem cells to tumor loci.Implications: This glioma study brings together actions of a normal epigenetic mechanism (JMJD3 activity) with dysfunctional activation of senescence-related processes, including secretion of SASP proinflammatory cytokines and stem cell tropism toward tumors. Mol Cancer Res; 13(4); 636–50. ©2015 AACR.
Database of Genomic Biomarkers for Cancer Drugs and Clinical Targetability in Solid Tumors
Authors: Dienstmann Rodrigo , Jang In Sock , Bot Brian , Friend Stephen , Guinney Justin 
Published: February 2015

AbstractSummary: Comprehensive genomic profiling is expected to revolutionize cancer therapy. In this Prospective, we present the prevalence of mutations and copy-number alterations with predictive associations across solid tumors at different levels of stringency for gene–drug targetability. More than 90% of The Cancer Genome Atlas samples have potentially targetable alterations, the majority with multiple events, illustrating the challenges for treatment prioritization given the complexity of the genomic landscape. Nearly 80% of the variants in rarely mutated oncogenes are of uncertain functional significance, reflecting the gap in our understanding of the relevance of many alterations potentially linked to therapeutic actions. Access to targeted agents in early clinical trials could affect treatment decision in 75% of patients with cancer. Prospective implementation of large-scale molecular profiling and standardized reports of predictive biomarkers are fundamental steps for making precision cancer medicine a reality. Cancer Discov; 5(2); 118–23. ©2015 AACR.
Asynchronous Brainstorming in an Industrial Setting
Authors: Paulus Paul B. , Korde Runa M. , Dickson Jubilee J. , Carmeli Abraham , Cohen-Meitar Ravit 
Published: February 2015

Objective: The goal of this study was to examine the effectiveness of brainwriting in an industrial setting. Background: Research has demonstrated that group interaction can inhibit idea generation. Written exchanges of ideas in groups have been found to be an effective way to increase idea generation. To our knowledge, no study has examined the potential of brainwriting for group idea generation in work settings or the impact of different sequences of group and individual idea generation. Method: Participants in a high-technology company participated in two brainwriting studies. In one study, participants generated ideas either first alone and then in a group or in the reverse order. In a second study, participants either generated as a group during the entire session or alternated individual ideation with a periodic review of the group’s ideas. Results: In the first study, participants who generated ideas first as a group and then as individuals performed best. In the first session, group writing also tended to lead to more ideas than did individual writing. In the second study, participants with periodic reviews performed best. Conclusion: The results suggest that alternation in individual and group brainwriting can enhance the number of ideas generated. The group-to-alone sequence is also beneficial since it allows group members to build on shared ideas. Application: This research indicates that collaborative idea sessions can be beneficial in work sessions if the brainwriting paradigm is used with an appropriate alternation of group ideation or review sessions with individual idea generation sessions.
Ultrasound Training in Otolaryngology Residencies
Authors: Meacham Ryan K. , Sebelik Merry E. 
Published: February 2015

Objective1) Estimate the current state of ultrasound experience among United States otolaryngology residents. 2) Describe self‐reported proficiency levels among senior residents in specific head and neck ultrasound techniques. 3) Identify deficiencies in ultrasound education and determine barriers to residents’ future use of ultrasound.MethodMidway through the academic year (January 2012), a link with an electronic survey was sent via email to every residency program director in the United States with a request to distribute the survey to the residents (PGY 1‐5). Survey responses were recorded anonymously and analyzed.ResultsA total of 123 residents responded. Of these, 62 (50.4%) had informal ultrasound training during residency, and 11 (9%) had attended a formal ultrasound course. Senior residents (PGY 4‐5, n = 52) had personally performed thyroid imaging (46.2%), thyroid FNA (32.7%), parathyroid imaging (19.2%), lymph node assessment (23.1%), salivary gland stone assessment (9.8%), and vocal cord mobility assessment (5.9%). Barriers to future ultrasound use included an inability to perform ultrasound (49.2%), cost of ultrasound machine (34.2%), and concerns about credentialing (32.5%). When asked if their exposure to ultrasound in residency was adequate, 27 (22.3%) said “yes,” 63 (52.1%) said “no,” and 31 (25.6%) said “unsure.”ConclusionUnited States otolaryngology residents have had variable exposure to ultrasound training. Less than half of senior residents have performed basic ultrasound skills. The biggest perceived impediment to future ultrasound use among prospective otolaryngologists is an inability to perform ultrasound, and relatively few otolaryngology residents feel their ultrasound training is adequate.
DISE: A 2‐Drug Comparison and Simultaneous Polysomnography
Authors: Carrasco Marina , Agostini Gabriela , Rodrigo Adelaida , Giner Pau , Gómez Fernando , Dalmau Jose 
Published: February 2015

ObjectiveThe aim of this study is to evaluate propofol and midazolam as sleep inducers in drug‐induced sleep endoscopy, for the topographic diagnosis of upper airway obstruction during sleep; compare one to the other as well as with natural sleep polysomnography results.MethodProspective series of 16 patients with suspected obstructive sleep apnea. Following physical examination and conventional polysomnography, sleep nasendoscopy along with simultaneous polysomnography was performed, obtaining apnea‐hypopnea index, lowest oxygen saturation, apnea durations, and sleep cycle stages. The procedure was done with propofol and with midazolam.ResultsThere were 15 men and 1 woman; the mean age was 42.7 years old, mean body mass index 26.86. Average drug‐induced sleep endoscopy (DISE) duration was 20 minutes with propofol and 14.3 minutes with midazolam. The induced‐sleep stage obtained was of phase 2. On 12 of the patients a whole‐night polysomnography study was performed and compared with sleep nasendoscopy results. Physical exam did not correlate with drug‐induced sleep findings. There was a good correlation between DISE results with both drugs (P <. 001). The average apnea‐hypopnea index was 16.38 for natural sleep, 28.63 for propofol, and 38.11 for midazolam.ConclusionObstruction areas are equivalent no matter which of the 2 mentioned sedatives are used. Although DISE is not entirely equivalent to an entire night sleep it can be considered comparable to most of the night sleep events.
Effects of formaldehyde inhalation on humoral immunity and protective effect of Nigella sativa oil
Authors: Sapmaz Hilal Irmak , Sarsılmaz Mustafa , Gödekmerdan Ahmet , Ögetürk Murat , Taş Ufuk , Köse Evren 
Published: February 2015

Aim: This study was carried out to determine the effects of formaldehyde (FA) inhalation on the humoral immunity of rats and the protective effect of Nigella sativa (NS) oil. Materials and Methods: The rats ( n = 33) were divided into five groups, with five animals in the control group (FA-free air) and seven in the other four groups. Group FA1 was exposed to FA (5 ppm), group FA + NS1 was treated with NS and exposed to FA (5 ppm), group FA2 was exposed to FA (10 ppm), and group FA + NS2 was treated with NS and exposed to FA (10 ppm). At the end of a 4-week study period, blood samples were collected. Enzyme-linked immunosorbent assay was used to determine the levels of serum total immunoglobulin A (IgA), total immunoglobulin M (IgM), total immunoglobulin G (IgG), and complement 3 (C3). Results: FA inhalation significantly increased serum IgA, IgM, and C3 levels and decreased serum IgG levels compared with the control group. NS administration decreased serum IgA, IgM, and C3 levels, which were induced by FA inhalation. Conclusion: FA inhalation significantly increased acute antibody responses and C3 levels in a dose-dependent manner compared with the control group. FA inhalation decreased the secondary immune response compared with the control group. Levels of acute antibody responses and complement following exposure to FA inhalation returned to normal following treatment with NS (immunoregulatory effect). However, NS did not affect the secondary immune response.
Gender dysphoria “cured” by status epilepticus
Authors: Parkinson John 
Published: February 2015

Objective: A case is described pointing to the biological factors in gender identity and personality structure. Methods: The history is presented of a transsexual patient who experienced a change of gender identity and personality following an episode of status epilepticus. Results: The patient presented as convincingly feminine and with features of Borderline Personality Disorder. She had an old brain injury. After a prolonged epileptic episode the Borderline features disappeared and the patient expressed a clearly male identity. Conclusions: The findings are equivocal: they may be seen both as reflecting a change in a neurologically structured identity and as a resetting of psychosocially acquired characteristics.
Atria are More Sensitive Than Ventricles to GS-458967-Induced Inhibition of Late Sodium Current
Authors: Burashnikov Alexander , Di Diego José M. , Goodrow Robert J. , Belardinelli Luiz , Antzelevitch Charles 
Published: February 2015

Introduction: The differential response of atrial and ventricular cells to late sodium channel current (late INa) inhibition has not been thoroughly investigated. The aim of the present study was to compare the atrioventricular differences in electrophysiological actions of GS-458967, a potent late INa blocker. Methods and Materials: Canine coronary-perfused atrial and ventricular preparations and isolated ventricular myocytes were used. Transmembrane action potentials were recorded using standard microelectrode recording techniques. Results: In coronary-perfused preparations paced at a cycle length (CL) of 500 ms, GS-458967 (100-300 nmol/L) significantly abbreviated action potential duration at 50% to 90% (APD50-90) in atria but not in the ventricles. GS-458967 (≥100 nmol/L) prolonged the effective refractory period (ERP) in atria due to the development of postrepolarization refractoriness (PRR) but did not alter ERP in the ventricles. The maximum rate of rise in the action potential upstroke (Vmax) was significantly reduced at concentrations ≥100 nmol/L in atria but not in the ventricles (CL = 300 ms). At slower pacing rates (CL = 2000 ms) and higher concentrations, GS-458967 (100-1000 nmol/L) still failed to abbreviate ventricular APD. However, when APD was prolonged by the rapidly activating delayed rectifier potassium channel blocker E-4031 (1 µmol/L), addition of 1 μmol/L GS-458967 abbreviated APD in the ventricles at slow rates. In contrast, GS-458967 (300 nmol/L) consistently abbreviated APD in untreated isolated ventricular myocytes. Conclusion: In canine coronary-perfused preparations, GS-458967 abbreviates APD, induces PRR, and reduces Vmax in atria but has no significant effect on these parameters in the ventricles, indicating an atrial-selective effect of GS-458967 on both peak and late INa-mediated parameters. In multicellular preparations, GS-458967 abbreviated ventricular APD only under long QT conditions, suggesting a pathology-specific action of GS-458967 in canine ventricular myocardium.
Can Hong Kong Chinese parents and their adolescent children benefit from an adapted UK parenting programme
Authors: Tsang Andrew Low Yiu 
Published: February 2015

Summary Sixty-five parents joined seven semi-structured group interviews sharing their experience of joining an adapted parenting programme for Hong Kong Chinese parents. The aim of the research was to explore parents’ outcome, parent–adolescent outcome of the parenting programme, as well as any culturally sensitive parenting strategies that were acceptable to Hong Kong Chinese parents. Findings The results indicate that Hong Kong Chinese parents perceived that the parenting programme was useful in improving communication with their adolescents, reducing conflict as well as supporting them to become confident parents of adolescents. Applications This study further illustrates the possible culturally sensitive elements of the Hong Kong Chinese parenting programme. It should focus on helping parents to reflect on their own parenting and help parents to learn granting autonomy to their adolescents, communication skills and conflict resolution skills.
Study of quality of life in the patients with personality neurotic development
Authors: Бочкарева  , Bochkareva N. , Кирюхина  , Kiryukhina S. , Подсеваткин  , Podsevatkin V. , Подсеваткина  , Podsevatkina S. 
Published: February 2015

Objective: To study the therapeutic effects of mexidol, hyperbaric oxygenation and timogena in complex treatment of patients with neurotic personality development and its impact on quality of life of patients.
Material and methods: The study included 142 patients with neurotic personality development. Patients of the first group were prescribed conventional therapy anxiolytic and antidepressant within 30 days. Patients of the second group were treated basic therapy and antioxidant immune correctors, sessions of hyperbaric oxygenation. Performance criteria for the final evaluation of the results of treatment were: reduction of the severity of psychiatric symptoms, improve mental and physical performance and social activity.
Results: In patients with neurotic personality development identified physical and mental exhaustion, re-duced intentions, low productivity at work and social exclusion, which is reflected in low rates of quality of life in social relations and in the psychological realm. These symptoms on a background of traditional anxiolytic and antidepressant therapy didn’t cropped, but compounded undesirable actions of these drugs. Combined anxiolytic and antidepressant combination with hyperbaric oxygenation and antioxidant immune correctors demonstrate the effectiveness of the therapeutic effects of metabolic therapy, which is manifested in a significant improvement in physical, mental and social functioning of patients and improve their quality of life.
Conclusions: The combination of paroxetine and diazepam with mexidol, hyperbaric oxygenation therapy, timogena can reduce the undesirable effects of antidepressants and benzodiazepine anxiolytics, increase efficiency of patients, their activity in communication, social inclusion and quality of life, indicating that the high efficacy and safety of this regimen.
Acupuncture and phototherapy efficiency for correction оf erectile dysfunction in the patients with neurotic depression
Authors: Дмитриенко  , Dmitrienko G. , Баисова  , Baisova E. , Череващенко  , Cherevashchenko Lyubov 
Published: February 2015

Sexual dysfunction in men, limiting their reproductive capabilities, prevent the achievement of marital and sexual life as a couple, break the stability of marriage, adversely affect mental health, lead to the development of neurotic and depressive disorders, alcohol abuse and antisocial behaviour.
In recent years, interest in the problem of vasculogenic erectile dysfunction has increased worldwide. But, despite this, many researchers believe that erectile dysfunction of vascular origin ranks second in frequency after disorders due to psychogenic factors.
Therapy of sexual disorders with neurotic depression has a number of obstacles due to absence of system-structural approach to sexopathologist syndromes. It is necessary to search new possibilities of correction of sexual disorders in patients with neurotic depression.
The purpose of this study was to develop and to justify pathogenetically the method of combined use of phototherapy and acupuncture for the correction of sexual disorders to improve the effect of treatment in men with neurotic depression.
80 patients with neurotic depression and erectile dysfunction prior to the application of medical facilities and at the end of treatment were examined. The results of treatment were: restoration of sexual function, decrease or disappearance of neurasthenic complaints (irritability, fatigue, headaches, dyssomnia, psycho-emotional, vegetative-vascular disorders, and others), normalization of libido and paraclinical data (psychological testing, electrophysiological parameters).