bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparison of ground reaction forces as running speed increases between male and female runners
Authors: Jiang Xinyan , Bíró István , Sárosi József , Fang Yufei , Gu Yaodong 
Published: July 2024

Introduction: The biomechanics associated with human running are affected by gender and speed. Knowledge regarding ground reaction force (GRF) at various running speeds is pivotal for the prevention of injuries related to running. This study aimed to investigate the gait pattern differences between males and females while running at different speeds, and to verify the relationship between GRFs and running speed among both males and females.Methods: GRF data were collected from forty-eight participants (thirty male runners and eighteen female runners) while running on an overground runway at seven discrete speeds: 10, 11, 12, 13, 14, 15 and 16 km/h.Results: The ANOVA results showed that running speed had a significant effect (p &lt; 0.05) on GRFs, propulsive and vertical forces increased with increasing speed. An independent t-test also showed significant differences (p &lt; 0.05) in vertical and anterior-posterior GRFs at all running speeds, specifically, female runners demonstrated higher propulsive and vertical forces than males during the late stance phase of running. Pearson correlation and stepwise multiple linear regression showed significant correlations between running speed and the GRF variables.Discussion: These findings suggest that female runners require more effort to keep the same speed as male runners. This study may provide valuable insights into the underlying biomechanical factors of the movement patterns at GRFs during running.
Differential protein repertoires related to sperm function identified in extracellular vesicles (EVs) in seminal plasma of distinct fertility buffalo (Bubalus bubalis) bulls
Authors: Badrhan Shiva , Karanwal Seema , Pal Ankit , Chera Jatinder Singh , Chauhan Vitika , Patel Aditya , Bhakat Mukesh , Datta Tirtha K. , Kumar Rakesh 
Published: July 2024

Buffalo bulls are backbone of Indian dairy industry, and the quality of semen donating bulls determine the overall production efficiency of dairy farms. Seminal plasma harbor millions of lipid bilayer nanovesicles known as extracellular vesicles (EVs). These EVs carry a heterogenous cargo of essential biomolecules including fertility-associated proteins which contribute to fertilizing potential of spermatozoa. In this study, we explored size, concentration, and complete proteome profiles of SP EVs from two distinct fertility groups to uncover proteins influencing bull fertility. Through Dynamic Light Scattering (DLS) it was found that purified EVs were present in 7–14 size exclusion chromatographic (SEC) fractions with sizes ranging from 146.5 to 258.7 nm in high fertile (HF) and low fertile (LF) bulls. Nanoparticle Tracking Analysis (NTA) confirmed the size of seminal EVs up to 200 nm, and concentrations varying from 2.84 to 6.82 × 1011 and 3.57 to 7.74 × 1011 particles per ml in HF and LF bulls, respectively. No significant difference was observed in size and concentration of seminal EVs between two groups. We identified a total of 1,862 and 1,807 proteins in seminal EVs of HF and LF bulls, respectively using high throughput LC-MS/MS approach. Out of these total proteins, 1,754 proteins were common in both groups and about 87 proteins were highly abundant in HF group while 1,292 were less abundant as compared to LF bulls. Gene ontology (GO) analysis, revealed that highly abundant proteins in HF group were mainly part of the nucleus and involved in nucleosome assembly along with DNA binding. Additionally, highly abundant proteins in EVs of HF group were found to be involved in spermatogenesis, motility, acrosome reaction, capacitation, gamete fusion, and cryotolerance. Two highly abundant proteins, protein disulfide-isomerase A4 and gelsolin, are associated with sperm-oocyte fusion and acrosome reaction, respectively, and their immunolocalization on spermatozoa may indicate that these proteins are transferred through EVs. Our evidences support that proteins in EVs and subsequently their presence on sperm, are strongly associated with sperm functions. Altogether, our investigation indicates that SPEVs possess crucial protein repertoires that are essential for enhancing sperm fertilizing capacity.
The value of metagenomic next-generation sequencing with different nucleic acid extracting methods of cell-free DNA or whole-cell DNA in the diagnosis of non-neutropenic pulmonary aspergillosis
Authors: Cai Xiaomin , Sun Chao , Zhong Huanhuan , Cai Yuchen , Cao Min , Wang Li , Sun Wenkui , Tao Yujian , Ma Guoer , Huang Baoju , Yan Shengmei , Zhong Jinjin , Wang Jiamei , Lu Yajie , Guan Yuanlin , Song Mengyue , Wang Yujie , Li Yuanyuan , Su Xin 
Published: July 2024

PurposeMetagenomic next-generation sequencing(mNGS) is a novel molecular diagnostic technique. For nucleic acid extraction methods, both whole-cell DNA (wcDNA) and cell-free DNA (cfDNA) are widely applied with the sample of bronchoalveolar lavage fluid (BALF). We aim to evaluate the clinical value of mNGS with cfDNA and mNGS with wcDNA for the detection of BALF pathogens in non-neutropenic pulmonary aspergillosis.MethodsmNGS with BALF-cfDNA, BALF-wcDNA and conventional microbiological tests (CMTs) were performed in suspected non-neutropenic pulmonary aspergillosis. The diagnostic value of different assays for pulmonary aspergillosis was compared.ResultsBALF-mNGS (cfDNA, wcDNA) outperformed CMTs in terms of microorganisms detection. Receiver operating characteristic (ROC) analysis indicated BALF-mNGS (cfDNA, wcDNA) was superior to culture and BALF-GM. Combination diagnosis of either positive for BALF-mNGS (cfDNA, wcDNA) or CMTs is more sensitive than CMTs alone in the diagnosis of pulmonary aspergillosis (BALF-cfDNA+CMTs/BALF-wcDNA+CMTs vs. CMTs: ROC analysis: 0.813 vs.0.66, P=0.0142/0.796 vs.0.66, P=0.0244; Sensitivity: 89.47% vs. 47.37%, P=0.008/84.21% vs. 47.37%, P=0.016). BALF-cfDNA showed a significantly greater reads per million (RPM) than BALF-wcDNA. The area under the ROC curve (AUC) for RPM of Aspergillus detected by BALF-cfDNA, used to predict “True positive” pulmonary aspergillosis patients, was 0.779, with a cut-off value greater than 4.5.ConclusionWe propose that the incorporation of BALF-mNGS (cfDNA, wcDNA) with CMTs improves diagnostic precision in the identification of non-neutropenic pulmonary aspergillosis when compared to CMTs alone. BALF-cfDNA outperforms BALF-wcDNA in clinical value.
Use of the word “evolution” in the time of a global pandemic
Authors: Boyd Sada M. , Freimuth Sara , Xue Carolyn , Lyons Brandon , Nagori Sana , Yu Michael , Lozano-Huntelman Natalie , Diamant Eleanor S. , Yeh Pamela J. 
Published: July 2024

IntroductionThe media can play a vital role in determining to what extent the general public understands scientific concepts. The understanding and acceptance of evolution are often influenced by factors beyond scientific knowledge alone, including education, politics, and media consumption habits. Although discussion of the COVID-19 pandemic and evolution had fixed itself in public discourse on many fronts, the media often avoided the usage of the word “evolve” and showed a preference for alternatives such as “emerge” or “mutate.”MethodsWe conducted a systematic analysis to gauge the prominence of the concept of evolution in media discussions across seven English-speaking countries. This involved assessing the frequency of the term “evolve” concerning viral evolution within newspaper articles using electronic databases. Of the 4,951 newspaper articles examined in this study, 11% discussed viral evolution. Out of those articles, 12% explicitly used the word “evolve.”ResultsWe found that countries did not significantly differ in their “evolve” usage, which may reflect similarities in views about COVID-19 and its evolution across countries or reliance on shared information sources when reporting on COVID-19. We also found that, as the pandemic progressed, the discussion on viral evolution as a topic had increased, but the frequency with which the word “evolve” was explicitly used had decreased.DiscussionThe COVID-19 pandemic has had a substantial impact on science and health news reporting. Although evolution plays a crucial role in the progression of the COVID-19 pandemic, the term “evolve” is not frequently highlighted in COVID-19 news coverage. Our research underscores the significant implications of language choice when describing evolutionary events, particularly in shaping the public’s understanding of evolution, both in general and in the context of pandemics and infectious diseases.
Cardiac (tele)rehabilitation in routine clinical practice for patients with coronary artery disease: protocol of the REHAB + trial
Authors: van Mierlo Rutger F. R. , Houben Vitalis J. G. , Rikken Sem A. O. F. , Gómez-Doblas Juan Jose , Lozano-Torres Jordi , van ’t Hof Arnoud W. J. 
Published: July 2024

IntroductionCardiac rehabilitation programs face the challenge of suboptimal participation, despite being a level Ia recommendation. Cardiac telerehabilitation, with its potential to engage patients who might otherwise not show interest, necessitates the adaption of existing center-based cardiac rehabilitation programs to facilitate rehabilitation at home. REHAB + is a mobile cardiac telerehabilitation program cocreated with patients and rehabilitation centers, aiming to future-proof cardiac rehabilitation and improve accessibility. The REHAB + application enables users to remotely communicate with their coach, receive on-demand feedback on health goal progression, and reduces the need for frequent in-person meetings at the cardiac rehabilitation center. The REHAB + study seeks to compare patient-related outcomes and characteristics of patients between those offered the option to participate in cardiac telerehabilitation and those attending center-based cardiac rehabilitation over a twelve-month period.MethodsThe REHAB + study is a multicenter, prospective, matched controlled, observational study that includes (N)STEMI patients eligible for cardiac rehabilitation. We aim to enroll 300 participants for cardiac telerehabilitation and 600 for center-based cardiac rehabilitation. Participants opting for cardiac telerehabilitation (REHAB+) will be matched with center-based cardiac rehabilitation participants. Additionally, characteristics of patients unwilling to participate in either center-based rehabilitation or telerehabilitation but are willing to share their demographics will be collected. The primary endpoint is quality of life measured with the SF-36 questionnaire at three and twelve months, with patient-related characteristics driving intervention choice as the most important secondary endpoint. Secondary endpoints include physical activity, modifiable risk factors, and digital health experience. The trial is registered at clinicaltrials.gov with registration number NCT05207072.DiscussionThe REHAB + trial is unique by offering patients freedom to choose between cardiac telerehabilitation and center-based rehabilitation. The integration of digital components into cardiac rehabilitation has the potential to complement behavioral change strategies for specific patient groups. Offering patients the option of cardiac telerehabilitation next to center-based rehabilitation could enhance overall cardiac rehabilitation participation rates.
Impact of mustard biodiesel and clove oil additives on particulate matter emission and carbon deposition in diesel engines
Authors: Bhangwar Sajjad , Luhur Muhammad Ramzan , Walasai Gordhan Das , Alghamdi Thamer A. H. 
Published: July 2024

Recent studies suggest that the world is facing an energy crisis due to the depletion of fossil fuel reserves. To combat this issue, researchers have turned to biodiesel, a renewable bioenergy source made from vegetable oils, microalgae, and animal fats. A recent study analysed engine parts’ particulate matter emissions and carbon deposition during the long-term use of mustard biodiesel and clove oil as antioxidants in a compression ignition engine. Three samples of fuels: DF (diesel fuel), B30 (30% mustard biodiesel and 70% DF), and biodiesel blended fuel with 3000 PPM in a single-cylinder CI engine. The use of 30% biodiesel in diesel fuel (B30) for the endurance test was based on a good mix. The engine was run for 100 h to investigate the particulate matter emissions and carbon deposition. The particulate matter emission data was collected every 25 h, and for carbon deposition, the engine’s fuel injector was turned off after 100 h of engine running. The results showed a reduction in particulate matter emissions of about 9.97%, 13.367%, 7.24%, 14.64%, 5.3%, 12.32%, 1.88%, and 7.99% for PM1, PM2.5, PM7, and PM10 in biodiesel blended fuel and biodiesel blended fuel with clove oil, respectively. The deposition of clove oil added to biodiesel blended fuel in the fuel injector has been reduced compared with the other fuels. Carbon deposition of the fuel injector was analysed through SEM and EDX tests, and the results showed that the carbon content in biodiesel blended fuel was lower than in diesel fuel. The deposition of clove oil added to biodiesel blended fuel in the fuel injector has been reduced compared with biodiesel blended fuel.
Analgesic efficacy of erector spinae plane block versus transversus abdominis plane block for laparoscopic cholecystectomy: a systematic review and meta-analysis of randomized controlled trial
Authors: Zewdu Dereje , Tantu Temesgen , Eanga Shamil , Tilahun Tamiru 
Published: July 2024

BackgroundAlthough laparoscopic cholecystectomy (LC) is a minimally invasive surgery, it can cause moderate to severe postoperative pain. Erector spinae plane (ESP) and transversus abdominis plane (TAP) blocks are considered effective means for pain management in such cases; however, there is inconclusive evidence regarding their analgesic efficacy. This meta-analysis aimed to compare the efficacy of ESP block and TAP block for pain control in LC.MethodsWe systematically searched Medline, PubMed, Scopus, Embase, and Google Scholar until 26 January 2024. All randomized clinical trials compared the efficacy of erector spinae plane block (ESPB) and transversus abdominis plane block (TAPB) for postoperative pain relief after LC. The primary outcomes were pain scores at rest and on movement at 1, 2, 6, 12, and 24 h postoperatively. The secondary outcomes were total opioid consumption, first analgesia request time, and rates of postoperative nausea and vomiting. We analyzed all the data using RevMan 5.4.ResultsA total of 8 RCTs, involving 542 patients (271 in the ESPB group and 271 in the TAPB group), were included in the analysis. The ESP block demonstrated statistically significant lower pain scores at rest and on movement than the TAP block at all-time points except at the 1st and 6th h on movement postoperatively; however, these differences were not considered clinically significant. Additionally, patients who received the ESP block required less morphine and had a longer time before requesting their first dose of analgesia. There were no significant differences in postoperative nausea and vomiting incidence between the two groups.ConclusionIn patients undergoing LC, there is moderate evidence to suggest that the ESP block is effective in reducing pain severity, morphine equivalent consumption, and the time before the first analgesia request when compared to the TAP block during the early postoperative period.Systematic review registration: PROSPERO CRD42024505635, https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024505635.
Efficacy and visual outcomes of the foldable capsular buckle scleral buckling in rhegmatogenous retinal detachment
Authors: Jiang Guohua , Lin Yuan , Chen Yan , Wu Huping 
Published: July 2024

ObjectiveTo investigate the difference in the effectiveness and refraction of the foldable capsular buckle (FCB) in rhegmatogenous retinal detachment (RRD).MethodsSix patients with simple RRD were treated for FCB scleral buckling at Xiamen Eye Center of Xiamen University from October 2023 to February 2024. The parameters assessed included demographic data, clinical data such as preoperative ocular axis, corneal endothelial count, macular foveal thickness, operative time, preoperative and final follow-up intro ocular pressure (IOP), retinal attachment status, and postoperative complications. Refractive change before and after surgery, including sphere, cylinder degree, spherical equivalent, and absolute spherical equivalent difference were compared.ResultsAll six patients with sound retinal reattachment after FCB scleral buckling, including two men and four women, mean age 41.33 ± 12.40 years old, duration before surgery onset to 7.17 ± 7.16 days, FCB mean operation time 36.67 ± 13.07 min, Preoperative IOP mean 13.35 ± 2.64 mmHg and mean 21.12 ± 8.09 mmHg of final follow-up IOP; there was no significant difference between preoperative IOP and follow-up IOP (p = 0.050). The preoperative sphere range was −6.25 to +2.50 D, and the cylinder range was −2.50 to +1.00 D; the absolute spherical equivalent difference before and after was 1.60 ± 1.69 degrees.ConclusionFCB can achieve retinal reattachment and restore visual function in cases of RRD. The shorter duration of external scleral buckle compression with FCB suggests that FCB scleral buckling holds greater promise in the clinical treatment of RRD caused by retinal tears.
Syndromic epidermolysis bullosa simplex subtype due to mutations in the KLHL24 gene: series of case reports in Russian families
Authors: Kotalevskaya Yulia Y. , Stepanov Vadim A. 
Published: July 2024

ObjectiveEpidermolysis bullosa simplex (EBS) is a common, well-characterized type of epidermolysis bullosa. However, some rare syndromic EBS phenotypes are not well described. The accumulation of clinical descriptions of patients with syndromic subtypes of EBS is important for understanding the natural history of the disease and assessing genotype–phenotype correlations.Case summaryWe present a series of case reports of the syndromic subtype of EBS associated with mutations in the KLHL24 gene in seven patients from four unrelated families. The clinical features of this rare phenotype in children and adult patients are described in detail. In two families, we revealed pathogenic variant c.1A &gt; G (p.Met1?) in the KLHL24 gene. The third family had c.3G &gt; A (p.Met1?) mutation, and the fourth family had a novel de novo variant c.23del (p.Arg8AsnfsTer2).ConclusionThe description of the clinical manifestations of the disease in two generations of EBS families with different genetic variants allows the assessment and prediction of the natural course and severity of the disease in these families, the risk of complications, and the planning of the amount of medical care necessary.
Safety and effectiveness of baloxavir marboxil and oseltamivir for influenza in children: a real-world retrospective study in China
Authors: Ge Xilin , Chen Yang , Wu Wei , Lu Jinmiao , Wang Yi , Li Zhiping 
Published: July 2024

ObjectivesTo evaluate the safety and effectiveness of baloxavir marboxil (baloxavir) and oseltamivir in pediatric influenza patients in China.MethodsPatients filling a prescription for baloxavir or oseltamivir within 48 h following an influenza-related outpatient visit were identified in Children's Hospital of Fudan University in China between March 2023 and December 2023. Outcomes were assessed after antiviral treatment and included the incidence of adverse reactions and the duration of fever and other flu symptoms.ResultsA total of 1430 patients infected with influenza A were collected and 865 patients (baloxavir: n = 420; oseltamivir: n = 445) finally included. The incidence of adverse reactions of nausea and vomiting was significantly different between the baloxavir group (2.38%) and the oseltamivir group (12.13%) [P &lt; 0.001, OR = 4.2526, 95%CI (2.0549, 9.6080)]. No differences in other adverse reactions were observed between the two groups. The mean duration of fever in baloxavir group (1.43d) was significantly shorter than that in oseltamivir group (2.31d) [P &lt; 0.001, 95%CI (0.7815, 0.9917)]. There were no differences in the mean duration of nasal congestion and runny nose, sore throat, cough, and muscle soreness between two groups.ConclusionsThe incidence of nausea and vomiting is lower with baloxavir compared to oseltamivir, and the duration for complete fever reduction is shorter with baloxavir than with oseltamivir. The results indicate that baloxavir is well tolerated and effective in Chinese children.
Integrating transcriptomics and metabolomics to reveal the protective effect and mechanism of Bushen Kangshuai Granules on the elderly people
Authors: Hu Jun , Yang Fengmin , Yang Guang , Pan Juhua , Tan Yumeng , Tang Yalin , Liu Yongmei , Zhang Hong , Wang Jie 
Published: July 2024

Background:Aging is characterized by a decline in the adaptability and resistance of the body. In this study, Bushen Kangshuai Granules (BKG), as a kind of Chinese herbal formula, was developed and shown to alleviate aging-related symptoms.Methods:Self-controlled study combined with RNA-seq and metabonomics were used to expound the efficacy and safety of BKG and revealed the regulation mechanism of BKG treating aging.In vitroexperiments were used to confirm the analytical results. The aging cell model of AC16 cells were treated with D-galactose. The RT-qPCR was used to detect the impact of BKG on telomere length. The DCFH-DA staining was used for detecting intracellular ROS. The targeted signaling pathway was selected and verified using Western blot.Results:After 8 weeks of treatment, BKG significantly reduced SOD level (p =0.046), TCM aging symptoms (p &lt;0.001) and TNF-α level (p =0.044) in the elderly participants. High-throughput sequencing showed that BKG reversed the expression of 70 and 79 age-related genes and metabolites, respectively. Further enrichment analysis indicated that BKG downregulated thePI3K-AKTsignaling pathway, extracellular matrix (ECM)-receptor interaction, and Rap1 signaling pathway, while up-regulating sphingolipid metabolism. The results ofin vitroexperiments show that, after D-gal treatment, the viability and telomere length of AC16 cells significantly decreased (p &lt;0.05), while the expression of ROS increased (p &lt;0.05), BKG significantly increased the telomere length of AC16 cells and reduced the level of ROS expression (p &lt;0.05). In addition, BKG decreased the expression of THBS1, PDGFRA, and EPS8L1(p &lt;0.05), consistent with the RNA-seq results. Our results also showed that BKG affectsPI3K-AKTsignaling pathway.Conclusion:BKG can significantly improve aging-related symptoms and increase SOD levels, which may be associated with the reversal of the expression of various aging-related genes. ThePI3K-AKTsignaling pathway and sphingolipid metabolism may be potential mechanisms underlying BKG anti-aging effects.