bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ex vivo identification and characterization of a population of CD13high CD105+ CD45− mesenchymal stem cells in human bone marrow
Authors: Muñiz Carmen , Teodosio Cristina , Mayado Andrea , Amaral Ana Teresa , Matarraz Sergio , Bárcena Paloma , Sanchez Maria Luz , Alvarez-Twose Iván , Diez-Campelo María , García-Montero Andrés C. , Blanco Juan F. , Del Cañizo Maria Consuelo , del Pino Montes Javier , Orfao Alberto 
Published: September 2015

AbstractIntroductionMesenchymal stem cells (MSCs) are multipotent cells capable of self-renewal and multilineage differentiation. Their multipotential capacity and immunomodulatory properties have led to an increasing interest in their biological properties and therapeutic applications. Currently, the definition of MSCs relies on a combination of phenotypic, morphological and functional characteristics which are typically evaluated upon in vitro expansion, a process that may ultimately lead to modulation of the immunophenotypic, functional and/or genetic features of these cells. Therefore, at present there is great interest in providing markers and phenotypes for direct in vivo and ex vivo identification and isolation of MSCs.MethodsMultiparameter flow cytometry immunophenotypic studies were performed on 65 bone marrow (BM) samples for characterization of CD13highCD105+CD45–cells. Isolation and expansion of these cells was performed in a subset of samples in parallel to the expansion of MSCs from mononuclear cells following currently established procedures. The protein expression profile of these cells was further assessed on (paired) primary and in vitro expanded BM MSCs, and their adipogenic, chondrogenic and osteogenic differentiation potential was also determined.ResultsOur results show that the CD13highCD105+CD45−immunophenotype defines a minor subset of cells that are systematically present ex vivo in normal/reactive BM (n = 65) and that display immunophenotypic features, plastic adherence ability, and osteogenic, adipogenic and chondrogenic differentiation capacities fully compatible with those of MSCs. In addition, we also show that in vitro expansion of these cells modulates their immunophenotypic characteristics, including changes in the expression of markers currently used for the definition of MSCs, such as CD105, CD146 and HLA-DR.ConclusionsBM MSCs can be identified ex vivo in normal/reactive BM, based on a robust CD13highCD105+and CD45−immunophenotypic profile. Furthermore, in vitro expansion of these cells is associated with significant changes in the immunophenotypic profile of MSCs.
Mesenchymal stromal cells enhance the engraftment of hematopoietic stem cells in an autologous mouse transplantation model
Authors: Fernández-García María , Yañez Rosa M. , Sánchez-Domínguez Rebeca , Hernando-Rodriguez Miriam , Peces-Barba Miguel , Herrera Guadalupe , O’Connor Jose E. , Segovia José C. , Bueren Juan A. , Lamana María L. 
Published: September 2015

Abstract
Introduction
Studies have proposed that mesenchymal stem cells (MSCs) improve the hematopoietic engraftment in allogeneic or xenogeneic transplants and this is probably due to the MSCs’ immunosuppressive properties. Our study aimed to discern, for the first time, whether MSC infusion could facilitate the engraftment of hematopoietic stem cells (HSCs) in autologous transplantations models, where no immune rejection of donor HSCs is expected.

Methods
Recipient mice (CD45.2) mice, conditioned with moderate doses of radiation (5-7 Gy), were transplanted with low numbers of HSCs (CD45.1/CD45.2) either as a sole population or co-infused with increasing numbers of adipose-derived-MSCs (Ad-MSCs). The influence of Ad-MSC infusion on the short-term and long-term engraftment of donor HSCs was investigated. Additionally, homing assays and studies related with the administration route and with the Ad-MSC/HSC interaction were conducted.

Results
Our data show that the co-infusion of Ad-MSCs with low numbers of purified HSCs significantly improves the short-term and long-term hematopoietic reconstitution of recipients conditioned with moderate irradiation doses. This effect was Ad-MSC dose-dependent and associated with an increased homing of transplanted HSCs in recipients’ bone marrow. In vivo and in vitro experiments also indicate that the Ad-MSC effects observed in this autologous transplant model are not due to paracrine effects but rather are related to Ad-MSC and HSC interactions, allowing us to propose that Ad-MSCs may act as HSC carriers, facilitating the migration and homing of the HSCs to recipient bone marrow niches.

Conclusion
Our results demonstrate that Ad-MSCs facilitate the engraftment of purified HSCs in an autologous mouse transplantation model, opening new perspectives in the application of Ad-MSCs in autologous transplants, including HSC gene therapy.
miR-17 promotes expansion and adhesion of human cord blood CD34+ cells in vitro
Authors: Yang Yuxia , Wang Saifeng , Miao Zhenchuan , Ma Wei , Zhang Yanju , Su Li , Hu Mengyu , Zou Junhua , Yin Yuxin , Luo Jianyuan 
Published: September 2015

Abstract
Introduction
We have recently found that miR-17 is necessary in the cell-extrinsic control of cord blood (CB) CD34+ cell function. Here, we demonstrated that the proper level of miR-17 is also necessary in the cell-intrinsic control of the hematopoietic properties of CB CD34+ cells.

Methods
The miR-17 overexpression and knockdown models were created using primary CB CD34+ cells transfected by the indicated vectors. Long-term culture, colony forming, adhesion and trans-well migration assays were carried out to investigate the function of miR-17 on CB CD34+ cells in vitro. NOD prkdc
scid
Il2rg
null
mice were used in a SCID repopulating cell assay to investigate the function of miR-17 on CB CD34+ cells in vivo. A two-tailed Student’s t-test was used for statistical comparisons.

Results
In vitro assays revealed that ectopic expression of miR-17 promoted long-term expansion, especially in the colony-forming of CB CD34+ cells and CD34+CD38− cells. Conversely, downregulation of miR-17 inhibited the expansion of CB CD34+ cells. However, the overexpression of miR-17 in vivo reduced the hematopoietic reconstitution potential of CB CD34+ cells compared to that of control cells. The increased expression of major adhesion molecules in miR-17 overexpressed CB CD34+ cells suggests that the adhesion between miR-17 overexpressed CB CD34+ cells and their niche in vivo is regulated abnormally, which may further lead to the reduced hematopoietic reconstitution capability of 17/OE cells in engrafted mice.

Conclusion
We conclude that the proper expression of miR-17 is required, at least partly, for normal hematopoietic stem cell–niche interaction and for the regulation of adult hematopoiesis.
Osteogenic differentiation of mesenchymal stromal cells in two-dimensional and three-dimensional cultures without animal serum
Authors: Castrén Eeva , Sillat Tarvo , Oja Sofia , Noro Ariel , Laitinen Anita , Konttinen Yrjö T , Lehenkari Petri , Hukkanen Mika , Korhonen Matti 
Published: September 2015

Abstract
Introduction
Bone marrow-derived mesenchymal stromal cells (MSCs) have been intensely studied for the purpose of developing solutions for clinical tissue engineering. Autologous MSCs can potentially be used to replace tissue defects, but the procedure also carries risks such as immunization and xenogeneic infection. Replacement of the commonly used fetal calf serum (FCS) with human platelet lysate and plasma (PLP) to support cell growth may reduce some of these risks. Altered media could, however, influence stem cell differentiation and we address this experimentally.

Methods
We examined human MSC differentiation into the osteoblast lineage using in vitro two- and three-dimensional cultures with PLP or FCS as cell culture medium supplements. Differentiation was followed by quantitative polymerase chain reaction, and alkaline phosphatase activity, matrix formation and matrix calcium content were quantified.

Results
Three-dimensional culture, where human MSCs were grown on collagen sponges, markedly stimulated osteoblast differentiation; a fourfold increase in calcium deposition could be observed in both PLP and FCS groups. PLP-grown cells showed robust osteogenic differentiation both in two- and three-dimensional MSC cultures. The calcium content of the matrix in the two-dimensional PLP group at day 14 was 2.2-fold higher in comparison to the FCS group (p < 0.0001), and at day 21 it was still 1.3-fold higher (p < 0.001), suggesting earlier calcium accumulation to the matrix in the PLP group. This was supported by stronger Alizarin Red staining in the PLP group at day 14. In two-dimesional PLP cultures, cellular proliferation appeared to decrease during later stages of differentiation, while in the FCS group the number of cells increased throughout the experiment. In three-dimensional experiments, the PLP and FCS groups behaved more congruently, except for the alkaline phosphatase activity and mRNA levels which were markedly increased by PLP.

Conclusions
Human PLP was at least equal to FCS in supporting osteogenic differentiation of human MSCs in two- and three-dimensional conditions; however, proliferation was inferior. As PLP is free of animal components, and thus represents reduced risk for xenogeneic infection, its use for human MSC-induced bone repair in the clinic by the three-dimensional live implants presented here appears a promising therapy option.
Small intestinal submucosa-derived extracellular matrix bioscaffold significantly enhances angiogenic factor secretion from human mesenchymal stromal cells
Authors: Lin Xin , Robinson Mikella , Petrie Tye , Spandler Veronica , Boyd W. Douglas , Sondergaard Claus Svane 
Published: September 2015

AbstractIntroductionThe in vivo therapeutic effect of mesenchymal stromal cells (MSCs) is currently believed to be tightly linked to their paracrine secretion ability. However, insufficient or imprecise cell delivery, low cell survival and retention post-transplant, along with harsh donor site microenvironments, are major barriers to the clinical success of MSC therapies. Here we tested a small intestinal submucosa (SIS)-derived extracellular matrix (ECM) bioscaffold augmented with MSCs, with the hypothesis that they will facilitate the precise delivery of increased numbers of MSCs therefore improving cell viability and retention.MethodsIn this study, we evaluated the secretion of angiogenic factors from three human MSC lines cultured on SIS ECM. We used human antibody array and enzyme-linked immunosorbent assay to measure the level of angiogenic factors released from MSCs when cultured on SIS ECM or regular tissue culture plastic. We tested MSCs cultured for three different time points.ResultsWe found that the SIS ECM culture environment can significantly enhance the release of several angiogenic factors when compared to MSCs cultured on standard tissue culture plastic. Specifically, vascular endothelial growth factor and interleukin-8 secretion was significantly increased at 24, 48 and 72 hours postseeding onto SIS ECM whereas vascular endothelial growth factor release for cells cultured on plastic surface remained the same during these time points. We also observed significant donor to donor variation in cytokine production.ConclusionsThis study demonstrates that MSCs transplanted onto a SIS ECM may greatly increase their therapeutic potential through an increase in pro-angiogenic cytokine release.
The immune response of stem cells in subretinal transplantation
Authors: Xian Bikun , Huang Bing 
Published: September 2015

AbstractStem cell transplantation is a potential curative treatment for degenerative diseases of the retina. Among cell injection sites, the subretinal space (SRS) is particularly advantageous as it is maintained as an immune privileged site by the retinal pigment epithelium (RPE) layer. Thus, the success of subretinal transplantation depends on maintenance of RPE integrity. Moreover, both embryonic stem cells (ESCs) and mesenchymal stem cells (MSCs) have negligible immunogenicity and in fact are immunosuppressive. Indeed, many studies have demonstrated that immunosuppressive drugs are not necessary for subretinal transplantation of stem cells if the blood-retinal barrier is not breached during surgery. The immunogenicity of induced pluripotent stem cells (iPSCs) appears more complex, and requires careful study before clinical application. Despite low rates of graft rejection in animal models, survival rates for ESCs, MSCs, and iPSCs in retina are generally poor, possibly due to resident microglia activated by cell transplantation. To improve graft survival in SRS transplantation, damage to the blood-retinal barrier must be minimized using appropriate surgical techniques. In addition, agents that inhibit microglial activation may be required. Finally, immunosuppressants may be required, at least temporarily, until the blood-retinal barrier heals. We review surgical methods and drug regimens to enhance the likelihood of graft survival after SRS transplantation.
An extension of the contraction mapping principle to Lipschitzian mappings
Authors: Udo-utun Xavier A , Siddiqui Zakawat U , Balla Mohammed Y 
Published: September 2015

AbstractVery recently, Udo-utun (Fixed Point Theory Appl. 2014:65, 2014) established a property possessed by contractive operators with nonempty fixed point sets and used it to prove the existence of fixed points of nonexpansive maps. We extend these results and prove the existence of fixed points for L-Lipschitzian maps that possess this property. Our results generalize and unify results concerning asymptotic fixed point theory of contractive mappings. An application in ordinary differential equations is illustrated via a formulation of the global existence theorem for initial value problems.
Tentacle: distributed quantification of genes in metagenomes
Authors: Boulund Fredrik , Sjören Anders , Kristiansson Erik 
Published: September 2015

Abstract

Background
In metagenomics, microbial communities are sequenced at increasingly high resolution, generating datasets with billions of DNA fragments. Novel methods that can efficiently process the growing volumes of sequence data are necessary for the accurate analysis and interpretation of existing and upcoming metagenomes.

Findings
Here we present Tentacle, which is a novel framework that uses distributed computational resources for gene quantification in metagenomes. Tentacle is implemented using a dynamic master-worker approach in which DNA fragments are streamed via a network and processed in parallel on worker nodes. Tentacle is modular, extensible, and comes with support for six commonly used sequence aligners. It is easy to adapt Tentacle to different applications in metagenomics and easy to integrate into existing workflows.

Conclusions
Evaluations show that Tentacle scales very well with increasing computing resources. We illustrate the versatility of Tentacle on three different use cases. Tentacle is written for Linux in Python 2.7 and is published as open source under the GNU General Public License (v3). Documentation, tutorials, installation instructions, and the source code are freely available online at: http://bioinformatics.math.chalmers.se/tentacle
Exhibition Reviews
Authors: Magee Carol , I. Williams Gwyneth 
Published: September 2015

Abstract
Tchotchke: Mass-Produced Sentimental Objects in Contemp orary Art, Gund Gallery, Kenyon College, Gambier, Ohio, 16 January–31 May 2015
Women Fashion Power: Not a Multiple Choice, Design Museum, London, UK , 29 October 2014–26 Ap ril 2015
Evolution of organizational recess: A study on general and specialist scopes<p>Örgütsel nişlerin evrimi: Genelci ve uzman örgütler üzerine bir araştırma
Authors: Erenel Fahri 
Published: September 2015

<p>Current research in Organizational Ecology argues that alongside positional characteristics of institutions, the scope of operations is also quintessential to understanding routines directing firm behaviour. In order to widen their scope, firms need to discover markets outside their current niches. By operating outside their boundaries and broadening their operational areas, firms attain a set of routines which facilitate entry into new markets and, thus, continue their operations more efficiently and effectively. On the other hand, despite positional advantages of a broad scope, niche expansions entail riskier organizational changes for generalist firms compared to specialist firms. This study endeavours to explain factors playing an active role during the process of becoming a generalist or specialist firm in light of population ecology and organizational inertia theories. Primarily, a literature review of related Turkish and English sources was conducted. A semi-structured interview was established to attain in-depth insight from managers in firms which adopted a specialist strategy.</p><p> </p><p><strong>Özet</strong></p><p>Örgütsel ekoloji ilgili güncel çalışmalarda, işletmenin konumsal özelliklerinin yanı sıra geniş bir faaliyet alanına sahip olmalarının da işletme davranışlarına yön veren rutinleri anlamak açısından önemli olduğu ileri sürülmektedir. İşletmelerin geniş bir faaliyet alanına sahip olabilmeleri için mevcut nişlerinin sınırları dışında yer alan pazarları da keşfetmeleri gerekir. Böylelikle geniş bir nişe sahip olan işletmeler, nişlerini daha da genişleterek, yeni pazarlara girişlerini kolaylaştıran rutin bir faaliyetler seti ile etkin ve verimli bir biçimde faaliyetlerine devam edebilirler. Bununla birlikte bu niş genişlemeleri, genelci örgütlerin geniş faaliyet alanlarının sunduğu konumsal avantaja rağmen uzman örgütlere kıyasla kendilerini dezavantajlı duruma düşüren tutum ve riskli örgütsel değişimleri de içerir. Bu çalışmada nüfus (popülasyon) ekolojisi ve yapısal atalet teorileri bağlamında işletmelerin genelci olma veya uzmanlaşma sürecinde etkin rol oynayan faktörler ortaya konmaya çalışılmıştır. Çalışma kapsamında öncelikle doküman analizi tekniği kullanılarak konuyla ilgili İngilizce ve Türkçe kaynaklar detaylı biçimde incelenmiştir. Elde edilen verilerden hareketle yarı yapılandırılmış görüşme soruları oluşturularak, uzmanlaşma stratejisini benimseyen işletmelerde yönetici pozisyonunda çalışanlarla derinlemesine görüşmeler yapılmıştır.</p>
The Effects of Extensive and Intensive Margins of FDI on Domestic Employment: Microeconomic Evidence from Italy
Authors: Bronzini Raffaello 
Published: September 2015

Abstract
In this paper we verify whether enterprises that have started to produce abroad have reduced employment at home after the first foreign investment (extensive margin). Next, we assess whether changes in foreign employment induce changes in domestic employment for a sample of multinationals that have already established activities abroad (intensive margin). Using matching method and diff-in-diffs estimates, we find that two years after the first foreign investment domestic employment of investing firms is slightly higher than that of domestic enterprises, but mainly among those that have undertaken horizontal foreign direct investment. In multinationals that have already activated foreign operations we find a positive relationship between foreign and domestic employment. Our findings suggest that the skill composition of domestic workforce does not change neither at the extensive nor at the intensive margin of FDI.
Dimensions of diversity: Mapping the field of media and communication studies by combining cognitive and material dimensions
Authors: Buhmann Alexander , Ingenhoff Diana , Lepori Benedetto 
Published: September 2015

AbstractIn this study we empirically map the field of media and communication studies (MCS) by focusing on relationships between cognitive dimensions (such as research topics and approaches) on the one hand and material dimensions (such as funding and institutionalization) on the other. Our analysis, which focuses on the field of MCS in Switzerland, identifies two clusters of research institutions representing distinct strands of research in the field. Results show how these two strands differ in terms of their resource base, institutional positioning and recognition, teaching and transfer activities, as well as activities of scientific production. Similarities and differences in these dimensions serve to explain the general evolution of the field.
Advertising in social network sites – Investigating the social influence of user-generated content on online advertising effects
Authors: Knoll Johannes , Schramm Holger 
Published: September 2015

AbstractIn today’s social online world there is a variety of interaction and participatory possibilities which enable web users to actively produce content themselves. This user-generated content is omnipresent in the web and there is growing evidence that it is used to select or evaluate professionally created online information. The present study investigated how this surrounding content affects online advertising by drawing from social influence theory. Specifically, it was assumed that web users sharing an interpersonal relationship (interpersonal influence) and/or a group membership (collective influence) with authors of user-generated content which appears next to advertising on the web page are more strongly influenced in their response to the advertising than unrelated users. These assumptions were tested in a 2 × 2 between-subject experiment with 118 students who were exposed to four different Facebook profiles that differed in terms of interpersonal connection to the source (existent/non-existent) and collective connection to the source (existent/non-existent). The results show a significant impact in the case of collective influence, but not in the case of interpersonal influence. The underlying mechanisms of this effect and implications of the results for online advertising are discussed.
Age differences in recall and liking of arousing television commercials
Authors: van der Goot Margot J. , van Reijmersdal Eva A. , Kleemans Mariska 
Published: September 2015

AbstractThis article examines whether there are differences between older and younger adults in recall and liking of arousing television commercials. As hypothesized, the experiment demonstrated that older adults remembered brands and products in calm commercials better than in arousing commercials, and they also liked calm commercials more. In contrast, younger adults remembered brands and products in arousing commercials better and they liked these commercials more. In addition, (curvi)linear relationships showed that for older adults arousal deteriorates their recall and liking, whereas for younger adults arousal – up to a certain point – is beneficial. These findings strongly suggest that advertising effects found in younger samples are unlikely to be the same for older target groups. An important practical implication is that it currently seems wise to make commercials targeted towards older adults calm instead of arousing when the aim is to generate brand recall and liking.
Bots or journalists? News sharing on Twitter
Authors: Larsson Anders Olof , Hallvard Moe 
Published: September 2015

AbstractOnline news sites have become an internet ‘staple’, but we know little of the forces driving the popularity of such sites in relation to what could be understood as the latest iteration of the web – social media services. This research in brief article discusses empirical results regarding the uses of Twitter for news sharing. Specifically, we present a comparative analysis of links emanating from the service at hand to a series of media outlets over a six-month period in two countries; Sweden and Norway. Focusing on linking practices among highly active Twitter accounts, we problematize the assumption that online communication involves two or more humans by directing attention to more or less automated ‘bot’ accounts. In sum, it is suggested that such automated accounts need to be dealt with more explicitly by researchers as well as practitioners interested in the popularity of online news as expressed through social media activity.