bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced Piezoelectric Properties of (Ba1−xCax)(Ti0.92Sn0.08)O3 Lead‐Free Ceramics
Authors: Zhu Li‐Feng , Zhang Bo‐Ping , Zhao Xiao‐Kun , Zhao Lei , Zhou Peng‐Fei , Li Jing‐Feng 
Published: October 2012

(Ba1−xCax)(Ti0.92Sn0.08)O3 (x = 0.00–0.06 mol) ceramics with a high relative density over 96% were prepared by a conventional sintering method at 1480°C. At room temperature, a polymorphic phase transition from orthorhombic phase to tetragonal phase was confirmed by the XRD patterns in the composition range of 0.04 ≤ x ≤ 0.06. A high piezoelectric coefficient d33 up to 568 pC/N was obtained at x = 0.05 mol, which is higher than the other component of (Ba,Ca)(Ti,Sn)O3 system. At the same time, the corresponding planar electromechanical coupling factor kp, converse piezoelectric coefficient dS/dE, and dielectric constant εr reach 47.7%, 1013 pm/V, and 23000, respectively. These results indicate that the (Ba,Ca)(Ti,Sn)O3 ceramics are a promising candidate to replace for the lead‐based piezoelectric materials.
Nanocrystalline/Nanosized Ni1−γFe2+γO4 Ferrite Obtained by Contamination with Fe During Milling of NiO–Fe2O3 Mixture. Structural and Magnetic Characterization
Authors: Marinca Traian F. , Chicinaş Ionel , Isnard Olivier , Popescu Violeta 
Published: October 2012

The nanocrystalline nickel ferrite (NiFe2O4) was synthesized by reactive milling starting from equimolar mixture of oxides. The iron contamination during milling leads to a solid state reaction between Fe and NiFe2O4 spinel. This reaction starts for a milling time longer than 30 h. A mixed nickel–iron ferrite (Ni1−γFe2+γO4) and elemental Ni are obtained. The evolution of the nickel–iron mixed ferrite during milling and its properties were investigated using X‐ray diffraction, Fourier Transform Infrared Spectroscopy (FTIR), Laser Particles Size Analyzer and magnetic measurements. Annealing treatment (350°C/4 h in vacuum) is favorable to the reaction between phases. Replacement of Ni2+ cations by iron cations provided by contamination leads to the increase of lattice parameter value of the spinel structure. The magnetization of the nickel–iron mixed ferrite newly formed is larger than the nickel ferrite magnetization (13.6 μB/f.u. and 6.22 μB/f.u., respectively), due to the magnetic moment of Fe2+ cation which is double as compared to the Ni2+ cation. Magnetization of the milled samples decreases during milling due to the structural changes induced by milling in the nickel–iron mixed ferrite. The annealing induces a reordering of the cations which leads to a larger magnetization.
Implant decontamination during surgical peri‐implantitis treatment: a randomized, double‐blind, placebo‐controlled trial
Authors: de Waal Yvonne C. M. , Raghoebar Gerry M. , Huddleston Slater James J. R. , Meijer Henny J. A. , Winkel Edwin G. , van Winkelhoff Arie Jan 
Published: October 2012

AbstractAimThe objective of this randomized, double‐blind, placebo‐controlled trial was to study the effect of implant surface decontamination with chlorhexidine (CHX)/cetylpyridinium chloride (CPC) on microbiological and clinical parameters.Material & MethodsThirty patients (79 implants) with peri‐implantitis were treated with resective surgical treatment consisting of apically re‐positioned flap, bone re‐contouring and surface debridement and decontamination. Patients were randomly allocated to decontamination with 0.12% CHX + 0.05% CPC (test‐group) or a placebo‐solution (without CHX/CPC, placebo‐group). Microbiological parameters were recorded during surgery; clinical and radiographical parameters were recorded before (pre‐) treatment (baseline), and at 3, 6 and 12 months after treatment.ResultsNine implants in two patients in the placebo‐group were lost due to severe persisting peri‐implantitis. Both decontamination procedures resulted in significant reductions of bacterial load on the implant surface, but the test‐group showed a significantly greater reduction than the placebo‐group (log 4.21 ± 1.89 versus log 2.77 ± 2.12, p = 0.006). Multilevel analysis showed no differences between both groups in the effect of the intervention on bleeding, suppuration, probing pocket depth and radiographical bone loss over time.ConclusionImplant surface decontamination with 0.12% CHX + 0.05% CPC in resective surgical treatment of peri‐implantitis leads to a greater immediate suppression of anaerobic bacteria on the implant surface than a placebo‐solution, but does not lead to superior clinical results. The long‐term microbiological effect remains unknown.
Identification of phosphorylation sites in the COOH‐terminal tail of the μ‐opioid receptor
Authors: Chen Ying‐Ju , Oldfield Sue , Butcher Adrian J. , Tobin Andrew B. , Saxena Kunal , Gurevich Vsevolod V. , Benovic Jeffrey L. , Henderson Graeme , Kelly Eamonn 
Published: October 2012

AbstractPhosphorylation is considered a key event in the signalling and regulation of the μ opioid receptor (MOPr). Here, we used mass spectroscopy to determine the phosphorylation status of the C‐terminal tail of the rat MOPr expressed in human embryonic kidney 293 (HEK‐293) cells. Under basal conditions, MOPr is phosphorylated on Ser363 and Thr370, while in the presence of morphine or [D‐Ala2, NMe‐Phe4, Gly‐ol5]‐enkephalin (DAMGO), the COOH terminus is phosphorylated at three additional residues, Ser356, Thr357 and Ser375. Using N‐terminal glutathione S transferase (GST) fusion proteins of the cytoplasmic, C‐terminal tail of MOPr and point mutations of the same, we show that, in vitro, purified G protein‐coupled receptor kinase 2 (GRK2) phosphorylates Ser375, protein kinase C (PKC) phosphorylates Ser363, while CaMKII phosphorylates Thr370. Phosphorylation of the GST fusion protein of the C‐terminal tail of MOPr enhanced its ability to bind arrestin‐2 and ‐3. Hence, our study identifies both the basal and agonist‐stimulated phospho‐acceptor sites in the C‐terminal tail of MOPr, and suggests that the receptor is subject to phosphorylation and hence regulation by multiple protein kinases.
Analysis of the daily changes of melatonin receptors in the rat liver
Authors: Venegas Carmen , García José A. , Doerrier Carolina , Volt Huayqui , Escames Germaine , López Luis C. , Reiter Russel J. , Acuña‐Castroviejo Darío 
Published: October 2012

AbstractMelatonin membrane (MT1 and MT2) and nuclear (RORα) receptors have been identified in several mammalian tissues, including the liver. The mechanisms regulating hepatic melatonin receptors are yet unknown. This study investigated whether these receptors exhibit daily changes and the effects of melatonin on their levels. Our results show that mRNAs for MT1/MT2 receptors exhibit circadian rhythms that were followed by rhythms in their respective protein levels; the acrophases for the two rhythms were reached at 04:00 and 05:00 hr, respectively. Pinealectomy blunted the rhythms in both mRNAs and protein levels. In contrast, mRNA and protein levels of nuclear receptor RORα increased significantly after pinealectomy. The cycles of the latter receptor also exhibited circadian rhythms which peaked at 03:00 and 03:45 hr, respectively. Melatonin administration (10–200 mg/kg) increased in a dose‐dependent manner the protein content of MT1/MT2 receptors, with no effects on RORα. Lunzindole treatment, however, did not affect melatonin receptor expression or content of either the membrane or nuclear receptors. Together with previously published findings which demonstrated the intracellular distribution of melatonin in rat liver, the current results support the conclusion that the circadian rhythms of MT1/MT2 and RORα receptors are under the control of the serum and intracellular melatonin levels. Moreover, the induction of MT1/MT2 receptors after the administration of high doses of melatonin further suggests that the therapeutic value of melatonin may not be restricted to only low doses of the indoleamine.
Reconstructing the evolutionary history of an endangered subspecies across the changing landscape of the Great Central Valley of California
Authors: Matocq Marjorie D. , Kelly Patrick A. , Phillips Scott E. , Maldonado Jesus E. 
Published: October 2012

AbstractIdentifying historic patterns of population genetic diversity and connectivity is a primary challenge in efforts to re‐establish the processes that have generated and maintained genetic variation across natural landscapes. The challenge of reconstructing pattern and process is even greater in highly altered landscapes where population extinctions and dramatic demographic fluctuations in remnant populations may have substantially altered, if not eliminated, historic patterns. Here, we seek to reconstruct historic patterns of diversity and connectivity in an endangered subspecies of woodrat that now occupies only 1–2 remnant locations within the highly altered landscape of the Great Central Valley of California. We examine patterns of diversity and connectivity using 14 microsatellite loci and sequence data from a mitochondrial locus and a nuclear intron. We reconstruct temporal change in habitat availability to establish several historical scenarios that could have led to contemporary patterns of diversity, and use an approximate Bayesian computation approach to test which of these scenarios is most consistent with our observed data. We find that the Central Valley populations harbour unique genetic variation coupled with a history of admixture between two well‐differentiated species of woodrats that are currently restricted to the woodlands flanking the Valley. Our simulations also show that certain commonly used analytical approaches may fail to recover a history of admixture when populations experience severe bottlenecks subsequent to hybridization. Overall our study shows the strength of combining empirical and simulation analyses to recover the history of populations occupying highly altered landscapes.
PFT1, a transcriptional Mediator complex subunit, controls root hair differentiation through reactive oxygen species (ROS) distribution in Arabidopsis
Authors: Sundaravelpandian Kalaipandian , Chandrika Nulu Naga Prafulla , Schmidt Wolfgang 
Published: October 2012

Summary

Root hair morphogenesis is driven by an amalgam of interacting processes controlled by complex signaling events. Redox processes and transcriptional control are critical for root hair development. However, the molecular mechanisms that integrate redox state and transcription are largely unknown.

To elucidate a possible role of transcriptional Mediators in root hair formation, we analyzed the Arabidopsis root hair phenotype of T‐DNA insertion lines that harbor homozygous mutations in genes encoding Mediator subunits.

Genetic evidence indicates that the Mediator subunits PFT1/MED25 and MED8 are critical for root hair differentiation, but act via separate mechanisms. Transcriptional profiling of pft1 roots revealed that PFT1 activates a subset of hydrogen peroxide (H2O2)‐producing class III peroxidases. pft1 mutants showed perturbed H2O2 and superoxide () distribution, suggesting that PFT1 is essential to maintain redox homeostasis in the root. Chemical treatments rescued the pft1 mutant phenotype, indicating that correct reactive oxygen species (ROS) distribution is an essential prerequisite for root hair differentiation. In addition, PFT1 positively regulates cell wall remodeling genes that are essential for root hair formation.

Our results demonstrate that PFT1 maintains ROS distribution which, in turn, controls root hair differentiation. Thus, our findings reveal a novel mechanism in which the Mediator controls ROS homeostasis by regulating the transcriptional machinery.
Application of CFD Simulation to Predicting Upper‐Room UVGI Effectiveness
Authors: Gilkeson Carl A , Noakes Catherine 
Published: October 2012

AbstractThis study outlines the potential for Computational Fluid Dynamics (CFD) simulation to be used to predict upper‐room ultraviolet germicidal irradiation (UVGI) effectiveness to aid system design and the development of future guidance. A numerical study of two wall‐mounted UVGI lamps in a mechanically ventilated test chamber is used to assess the influence of modeling parameters on prediction of dose distribution and microorganism inactivation. Irradiance fields for both UVGI fixtures are obtained via radiometry and implemented in the model. A series of sensitivity studies consider the importance of UVGI field accuracy and computational grid and turbulence model selection. Results show that 2D irradiance fields are sufficient for calculating dose and in‐activation, whereas a 1D field is inadequate for modeling purposes. Further parametric studies consider the effects of ventilation parameters, UVGI lamp configuration and microorganism susceptibility. These demonstrate the feasibility of modeling the interaction of the airflow and UV field in a room to quantify the dose distribution. Microorganism in‐activation can also be accomplished by employing passive scalars and species transport models, however, further validation data are necessary before this can be used to make reliable quantitative predictions.
Assessment of Meiotic Spindle Configuration and Post‐Warming Bovine Oocyte Viability Using Polarized Light Microscopy
Authors: Caamaño JN , Díez C , Trigal B , Muñoz M , Morató R , Martín D , Carrocera S , Mogas T , Gómez E 
Published: October 2012

ContentsThe objectives of this study were to assess the efficiency of polarized light microscopy (PLM) in detecting microtubule‐polymerized protein in in vitro‐matured bovine oocytes; to examine its effects on oocyte developmental competence; and to assess the meiotic spindle of in vitro‐matured oocytes after vitrification/warming and further assessment of oocyte developmental competence. In the first experiment, the presence of microtubule‐polymerized protein (MPP) was confirmed as a positive PLM signal detected in 99.1% of analysed oocytes (n = 115), which strongly correlated (r = 1; p < 0.0001) with the presence of MPP as confirmed by immunostaining. In the second experiment, oocytes (n = 651) were exposed or not (controls) to PLM for 10 min and then fertilized and cultured in vitro. Oocytes exposed to PLM did not significantly differ from controls with regard to cleavage, total blastocyst and expanded blastocyst rates and cell numbers. In the third experiment, meiotic spindles were detected in 145 of 182 oocytes (79.6%) following vitrification and warming. Interestingly, after parthenogenetic activation and in vitro culture, oocytes that displayed a positive PLM signal PLM(+) differed significantly from PLM(−) in cleavage and Day 8 blastocyst rates. These results suggest that polarized light microscopy is an efficient system to detect microtubule‐polymerized protein in in vitro‐matured bovine oocytes and does not exert detrimental effects on bovine oocyte developmental competence. Moreover, PLM could be used as a tool to assess post‐warming viability in vitrified bovine oocytes.
Measurement and correction of leaf open times in helical tomotherapy
Authors: Sevillano David , Mínguez Cristina , Sánchez Alicia , Sánchez‐Reyes Alberto 
Published: October 2012

Purpose:The binary multileaf collimator (MLC) is one of the most important components in helical tomotherapy (HT), as it modulates the dose delivered to the patient. However, methods to ensure MLC quality in HT treatments are lacking. The authors obtained data on the performance of the MLC in treatments administered in their department in order to assess possible delivery errors due to the MLC. Correction methods based on their data are proposed.Methods:Twenty sinograms from treatments delivered using both of the authors HT systems were measured and analyzed by recording the fluence collected by the imaging detector. Planned and actual sinograms were compared using distributions of leaf open time (LOT) errors, as well as differences in fluence reconstructed at each of the 51 projections into which the treatment planning system divides each rotation for optimization purposes. They proposed and applied a method based on individual leaf error correction and the increase in projection time to prevent latency effects when LOT is close to projection time. In order to analyze the dosimetric impact of the corrections, inphantom measurements were made for four corrected treatments.Results:The LOTs measured were consistent with those planned. Most of the mean errors in LOT distributions were within 1 ms with standard deviations of over 4 ms. Reconstructed fluences showed good results, with over 90% of points passing the 3% criterion, except in treatments with a short mean LOT, where the percentage of passing points was as low as 66%. Individual leaf errors were as long as 4 ms in some cases. Corrected sinograms improved error distribution, with standard deviations of over 3 ms and increased percentages of points passing 3% in the fluence per angle analysis, especially in treatments with a short mean LOT and those that were more subject to latency effects. The minimum percentage of points within 3% increased to 86%. In‐phantom measurements of the corrected treatments showed that, while treatments affected by latency effects were improved, those affected by individual leaf errors were not.Conclusions:Measurement of MLC performance in real treatments provides the authors with a valuable tool for ensuring the quality of HT delivery. The LOTs of MLC are very accurate in most cases. Sources of error were found and correction methods proposed and applied. The corrections decreased the amount of LOT errors. The dosimetric impact of these corrections should be evaluated more thoroughly using 3D dose distribution analysis.
Genome Sequence of Aureobasidium pullulans AY4, an Emerging Opportunistic Fungal Pathogen with Diverse Biotechnological Potential
Authors: Chan Giek Far , Bamadhaj Hasima Mustafa , Gan Han Ming , Rashid Noor Aini Abdul 
Published: October 2012

ABSTRACT

Aureobasidium pullulans
AY4 is an opportunistic pathogen that was isolated from the skin of an immunocompromised patient. We present here the draft genome of strain AY4, which reveals an abundance of genes relevant to bioindustrial applications, including biocontrol and biodegradation. Putative genes responsible for the pathogenicity of strain AY4 were also identified.
Genome Sequence of the Psychrophilic Deep-Sea Bacterium Moritella marina MP-1 (ATCC 15381)
Authors: Kautharapu Kumar B. , Jarboe Laura R. 
Published: October 2012

ABSTRACT

Moritella marina
MP-1 is a bacterial species known for its production of docosahexaenoic acid. We present the draft genome sequence of the type strain
Moritella marina
MP-1 (ATCC 15381), having 4,636,778 bp with a G+C content of 40.5% and consisting of 83 contigs.
Complete Genome Sequence of a Variant of Campylobacter jejuni NCTC 11168
Authors: Revez Joana , Schott Thomas , Rossi Mirko , Hänninen Marja-Liisa 
Published: October 2012

ABSTRACT

Campylobacter jejuni
NCTC 11168 is widely used in research, but at least two variants have been reported. The available genome was sequenced from a variant which later showed a different phenotype and gene expression profile. Here we present the complete genome sequence of a second variant of
C. jejuni
NCTC 11168.
Draft Genome Sequence of the Volatile Organic Compound-Producing Antarctic Bacterium Arthrobacter sp. Strain TB23, Able To Inhibit Cystic Fibrosis Pathogens Belonging to the Burkholderia cepacia Complex
Authors: Fondi Marco , Orlandini Valerio , Maida Isabel , Perrin Elena , Papaleo Maria Cristiana , Emiliani Giovanni , de Pascale Donatella , Parrilli Ermenegilda , Tutino Maria Luisa , Michaud Luigi , Lo Giudice Angelina , Fani Renato 
Published: October 2012

ABSTRACT

Arthrobacter
sp. strain TB23 was isolated from the Antarctic sponge
Lissodendoryx nobilis
. This bacterium is able to produce antimicrobial compounds and volatile organic compounds (VOCs) that inhibit the growth of other Antarctic bacteria and of cystic fibrosis opportunistic pathogens, respectively. Here we report the draft genome sequence of
Arthrobacter
sp. TB23.
Complete Genome Sequence of Mycobacterium vaccae Type Strain ATCC 25954
Authors: Ho Yung S. , Adroub Sabir A. , Abadi Maram , Al Alwan Bader , Alkhateeb Reham , Gao Ge , Ragab Alaa , Ali Shahjahan , van Soolingen Dick , Bitter Wilbert , Pain Arnab , Abdallah Abdallah M. 
Published: October 2012

ABSTRACT

Mycobacterium vaccae
is a rapidly growing, nontuberculous
Mycobacterium
species that is generally not considered a human pathogen and is of major pharmaceutical interest as an immunotherapeutic agent. We report here the annotated genome sequence of the
M. vaccae
type strain, ATCC 25954.
Genome Sequences of Two Freshwater Betaproteobacterial Isolates, Limnohabitans Species Strains Rim28 and Rim47, Indicate Their Capabilities as Both Photoautotrophs and Ammonia Oxidizers
Authors: Zeng Yonghui , Kasalický Vojtěch , Šimek Karel , Koblížek Michal 
Published: October 2012

ABSTRACT

Betaproteobacterial genus
Limnohabitans
represents an important part of freshwater bacterioplankton. Here, we report genome sequences of two
Limnohabitans
isolates, Rim28 and Rim47. They contain a complete photosynthesis gene cluster, RuBisCO, CO dehydrogenase, ammonia monooxygenase, and sulfur-oxidizing genes, which indicates a great metabolic versatility of the
Limnohabitans
species.
Draft Genome Sequence of Paenibacillus sp. Strain OSY-SE, a Bacterium Producing the Novel Broad-Spectrum Lipopeptide Antibiotic Paenibacterin
Authors: Huang En , Guo Yaoqi , Yousef Ahmed E. 
Published: October 2012

ABSTRACT

A strain of
Paenibacillus
sp., OSY-SE, was isolated from soil and found to produce a novel lipopeptide antibiotic. The antibiotic, paenibacterin, is active against Gram-negative and Gram-positive bacterial pathogens. Paenibacterin is biosynthesized by a nonribosomal peptide synthetase pathway. Here we report the draft genome sequence of
Paenibacillus
sp. OSY-SE.
Genome Sequence of Rhizobium sp. Strain CCGE510, a Symbiont Isolated from Nodules of the Endangered Wild Bean Phaseolus albescens
Authors: Servín-Garcidueñas Luis E. , Rogel Marco A. , Ormeño-Orrillo Ernesto , Delgado-Salinas Alfonso , Martínez-Romero Julio , Sánchez Federico , Martínez-Romero Esperanza 
Published: October 2012

ABSTRACT

We present the genome sequence of
Rhizobium
sp. strain CCGE510, a nitrogen fixing bacterium taxonomically affiliated with the
R. leguminosarum
-
R. etli
group, isolated from wild
Phaseolus albescens
nodules grown in native pine forests in western Mexico.
P. albescens
is an endangered bean species phylogenetically related to
P. vulgaris
. In spite of the close host relatedness,
Rhizobium
sp. CCGE510 does not establish an efficient symbiosis with
P. vulgaris
. This is the first genome of a
Rhizobium
symbiont from a
Phaseolus
species other than
P. vulgaris
, and it will provide valuable new insights about symbiont-host specificity.
Complete Genome Sequence of Klebsiella pneumoniae 1084, a Hypermucoviscosity-Negative K1 Clinical Strain
Authors: Lin Ann-Chi , Liao Tsai-Lien , Lin Yi-Chun , Lai Yi-Chyi , Lu Min-Chi , Chen Ying-Tsong 
Published: October 2012

ABSTRACT

We report the complete genome sequence of
Klebsiella pneumoniae
1084, a hypermucoviscosity-negative K1 clinical strain. Sequencing and annotation revealed a 5,386,705-bp circular chromosome (57.4% G+C content), which contains 4,962 protein-coding genes, 80 tRNA genes, and 25 rRNA genes.
Draft Genome Sequence of Pontibacter sp. nov. BAB1700, a Halotolerant, Industrially Important Bacterium
Authors: Joshi M. N. , Sharma A. C. , Pandya R. V. , Patel R. P. , Saiyed Z. M. , Saxena A. K. , Bagatharia S. B. 
Published: October 2012

ABSTRACT

Pontibacter
sp. nov. BAB1700 is a halotolerant, Gram-negative, rod-shaped, pink-pigmented, menaquinone-7-producing bacterium isolated from sediments of a drilling well. The draft genome sequence of the strain, consisting of one chromosome of 4.5 Mb, revealed vital gene clusters involved in vitamin biosynthesis and resistance against various metals and antibiotics.