bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract B66: Activation of Wnt/β-catenin signaling enhances TRAIL-dependent apoptosis in human melanoma
Authors: Zimmerman Zachary F. , Chien Andy J. , Moon Randall T. 
Published: March 2013

Abstract
TNF receptor apoptosis inducing ligand (TRAIL/Apo2L) can induce apoptosis in tumor cells with limited toxicity to normal tissue. TRAIL based therapy is promising; however, melanomas are often resistant to TRAIL induced apoptosis. Wnt/β-catenin signaling in melanoma is linked to patient survival, apoptosis, and pathogenesis. In this study, we investigated the effect of activating Wnt/β-catenin signaling in melanoma with Wnt ligand or small molecules on sensitivity to TRAIL induced apoptosis. Treatment of melanoma cell lines with the canonical Wnt ligand Wnt3a strongly enhanced apoptosis induced by recombinant human TRAIL at doses as low as 10 ng/mL as measured by binding of Annexin V or cleavage of caspase 3 and PARP1. Knockdown by siRNA of β-catenin, the ultimate downstream effector of the Wnt/β-catenin pathway completely abrogated this effect. Treatment with the GSK3-β inhibitor CHIR99021, a small molecule activator of the Wnt pathway, also enhanced sensitivity to TRAIL. Furthermore, the HMG-COA reductase inhibitor simvastatin, a known Wnt enhancer, significantly increased TRAIL sensitivity in a β-catenin dependent fashion. Treatment of melanoma cells with Wnt3a resulted in increased levels of pro-apoptotic proteins BIM and PUMA as well as reduced expression of the anti-apoptotic protein MCL-1. Together these results suggest that activation of the Wnt/β-catenin pathway may be a promising novel strategy to overcome resistance to TRAIL in melanoma and warrants exploration of combinatorial therapy with Wnt and TRAIL agonists.
Abstract PR1: NKX2-1/TITF1/TTF-1-induced ROR1 is required to sustain EGFR survival signaling in lung adenocarcinoma
Authors: Yamaguchi Tomoya , Yanagisawa Kiyoshi , Sugiyama Ryoji , Hosono Yasuyuki , Shimada Yukako , Arima Chinatsu , Kato Seiichi , Tomida Shuta , Suzuki Motoshi , Osada Hirotaka , Takahashi Takashi 
Published: March 2013

Abstract
Emerging evidence, though currently sparse, suggests that “lineage-specific transcription factors” with developmental roles in normal progenitor cells of particular lineages may also confer dependency for survival to certain types of cancer cells. Accumulated evidence indicates that NKX2-1, a lineage-specific transcription factor also known as TITF1 and TTF-1, which is expressed in a major fraction of lung adenocarcinomas, plays essential roles in peripheral lung development. We previously identified NKX2-1 as a lineage-survival oncogene in lung adenocarcinoma, while other investigators reached similar conclusions through genome-wide searches for focal genomic aberrations. Previous findings including ours thus clearly indicate the requirement of sustained NKX2-1 expression for lung adenocarcinoma survival, while it remains elusive how NKX2-1 mediates survival signals.
In the present study, we show that NKX2-1 induces the expression of the receptor tyrosine kinase-like orphan receptor 1 (ROR1), which in turn sustains a favorable balance between prosurvival PI3K-AKT and proapoptotic p38 signaling, in part through ROR1 kinase-dependent c-Src activation. Interestingly, ROR1 was also identified as a receptor tyrosine kinase with a “sustainer role” for the interaction of EGFR-ERBB3 and ERBB3 phosphorylation in a ROR1 kinase-independent manner, consequentially leading to PI3K activation. It was also of note that ROR1 knockdown effectively inhibited lung adenocarcinoma cell lines irrespective of their EGFR status, including those with resistance to the EGFR tyrosine kinase inhibitor gefitinib. Secondary EGFR mutation, MET amplification, and HGF overexpression may arise in lung adenocarcinomas in patients undergoing EGFR-TKI treatment, leading to treatment resistance. The existence of such diverse mechanisms makes it difficult to predict which should be targeted to prevent expansion of resistant clones. From a clinical point of view, it is thus of particular interest that ROR1 inhibition appears to be effective for treatment of gefitinib-resistant lung adenocarcinomas with various resistance mechanisms.
Taken together, the present findings identify ROR1 as an “Achilles' heel” in lung adenocarcinomas. Future development of novel therapeutic means including ROR1-specific antibodies and small molecules that inhibit both or either of the two distinct prosurvival signal-sustaining functions is greatly anticipated for attempts to reduce the intolerable death toll from currently “hard-to-cure” lung adenocarcinomas.
This proffered talk is also presented as Poster A28.
Advanced Intimal Hyperplasia Without Luminal Narrowing of Leptomeningeal Arteries in CADASIL
Authors: Dong Hairong , Ding Haixia , Young Kelly , Blaivas Mila , Christensen Paul J. , Wang Michael M. 
Published: March 2013

Background and Purpose—
Leptomeningeal artery abnormalities in Cerebral Autosomal–Dominant Arteriopathy with Subcortical Infarcts and Leukoencephalopathy (CADASIL) have not been extensively characterized. We quantified substructure and diameter of leptomeningeal arteries in CADASIL compared with age-matched controls and the very old; in addition, we characterized intimal thickening in CADASIL using immunohistochemistry.

Methods—
Frontal and temporal cortex of 6 genetically proven CADASIL brains (average age, 66 years), 6 controls without symptoms of cerebrovascular disease, and 6 very old brains (average age, 89 years) were examined for leptomeningeal artery intimal, medial, and adventitial thickness; inner diameter; and sclerotic index and for smooth muscle markers.

Results—

The intima of CADASIL arteries was thickened 5-fold compared with controls and the very aged (
P
<0.0001). Medial thickness was lower in CADASIL compared with controls and the very old (
P
<0.01). The adventitia was not significantly increased in CADASIL compared with age-matched controls. Arterial diameters were not smaller in CADASIL compared with controls. Sclerotic index was significantly increased in CADASIL compared with other groups (
P
<0.00001). Intimal cells in CADASIL expressed smooth muscle actin, S100A4, and vimentin but not desmin.

Conclusions—
Principle changes of leptomeningeal arteries in CADASIL include intimal thickening and medial thinning, but not luminal narrowing. Smooth muscle–like cells participate in neointimal thickening of CADASIL arteries.
Perepiska [Letters], Mikhail Lifschitz and György Lukács, Moscow: Grundrisse, 2011; Pisma V. Dostalu, V. Arslanovu, M. Mikhailovu [Letters to V. Dostal, V. Arslanov, M. Mikhailov], Mikhail Lifschitz, Moscow: Grundrisse, 2011
Authors: Pavlov Evgeni V. 
Published: March 2013

Abstract
The two volumes of letters by Mikhail Lifschitz, recently published in Russian, reveal for the first time certain aspects of his relationship with György Lukács and his general intellectual and cultural role in the long history of Soviet aesthetics. The first volume contains all the known letters between Lifschitz and Lukács; the second volume contains the letters from Lifschitz to three of his younger colleagues. Both volumes throw considerable light on the development of Marxist aesthetics in the Soviet Union from the 1930s to the 1980s. The extensive commentaries and notes provide context for the various discussions in the letters and situate the main issues under consideration within the wider historical and social context of the period.
A New Sensor Platform for Investigating Turbulence in Stratified Coastal Environments
Authors: Bouffard Damien , Lemmin Ulrich 
Published: March 2013

Abstract
Characterizing and quantifying vertical exchange processes is essential for understanding physical and biological dynamics in stratified lakes and oceans. Unfortunately, the role of mixing is still poorly understood because of the challenges of conducting field research on small-scale turbulence, especially in the vicinity of a thermocline. This study presents a new moored sensor platform that was designed to investigate small-scale turbulence structures in time and space. The objective is to determine all terms of the turbulent energy equation separately and simultaneously. The platform is equipped with a microstructure package for measuring shear, temperature, and temperature gradients, as well as with a vertical array of high-precision thermistor probes and acoustic Doppler velocimeters. The platform can be moved vertically in the water column using a bottom-resting winch that is connected to a shore station by a 1800-m-long communication cable. This cable allows real-time data access and control of the winch, and thus optimization of the measurement strategy. A field study in Lake Geneva, located between Switzerland and France, shows that this system is ideally suited for the analysis of the dynamics of baroclinic motions, such as internal Kelvin waves. First results indicate a clear relationship between low Richardson number and elevated dissipation, and suggest a mean flux Richardson number Rf = 0.14 ± 0.4. However, this measurement campaign was not as conclusive for the reason of the variability of Rf.
Preformed vs Intraoperative Bending of Titanium Mesh for Orbital Reconstruction
Authors: Strong E. Bradley , Fuller Scott C. , Wiley David F. , Zumbansen Janina , Wilson M. D. , Metzger Marc C. 
Published: March 2013

ObjectiveThe most accurate orbital reconstructions result from an anatomic repair of the premorbid orbital architecture. Many different techniques and materials have been used; unfortunately, there is currently no optimal method. This study compares the use of preformed vs intraoperative bending of titanium mesh for orbital reconstruction in 2‐wall orbital fractures.Study DesignCadaver‐based study.SettingUniversity hospital.Subjects and MethodsPreinjury computed tomography scans were obtained in 15 cadaveric heads (30 orbits). Stereolithographic (STL) models were fabricated for 5 of the specimens (10 orbits). Two wall fractures (lamina papyracea and floor) were then generated in all orbits. Surgical reconstruction was performed in all orbits using 1 of 3 techniques (10 orbits each): (1) patient‐specific implant molded from the preinjury STL model, (2) titanium mesh sheet bent freehand, and (3) preformed titanium mesh. Each technique was evaluated for orbital volume correction, contour accuracy, ease of use, and cost.ResultsNo difference in volume restoration was found between the 3 techniques. Patient‐specific implants had the greatest contour accuracy, poor ease of use, and highest cost. Freehand bending implants had the poorest contour accuracy, acceptable ease of use, and lowest cost. Preformed mesh implants had intermediate contour accuracy, excellent ease of use, and low cost.ConclusionAll 3 techniques provide equivalent orbital volume correction. However, preformed mesh implants have many advantages based on contour accuracy, ease of use, and relative cost.
The Performance of Quantiferon-TB Gold in-Tube (QFT-IT) Test Compared to Tuberculin Skin Test (TST) in Detecting Latent Tuberculosis Infection (LTBI) in the Presence of HIV Coinfection in a High TB-Burden Area with BCG-Vaccinated Population
Authors: James Pratibha Malini , Ganaie Feroze Ahmed , Kadahalli Ravikumar Lingegowda 
Published: March 2013

Objectives: To compare quantiferon-TB gold “in tube” (QFT-IT) with the conventional tuberculin skin test (TST) for the diagnosis of latent tuberculosis infection (LTBI) in HIV-infected adults in a setting highly endemic for tuberculosis with BCG vaccinated population in Bangalore, Karnataka, India. Patients and Methods: The study population (100 BCG-vaccinated adults) was divided into 4 groups: HIV patients with TB patient contact, HIV patients with past history of TB, TB patients (positive control), and healthy volunteers (negative control). Results: Overall agreement between TST and QFT-IT was 52.4% (κ = 0.22). Increasing the TST cutoff value from 5 mm to 10 mm among HIV-positive groups resulted in better agreement 62.5% in Group 1 and 81.2% in Group 2. Discussion: In a setting with high TB prevalence, the TST gives increased false positives in HIV-positive groups if 5 mm is used as the cutoff value. This could be minimized by increasing the cutoff to 10 mm. Conclusion: The 2-step approach (initial testing of all cases with TST and confirmation of only those positive in TST by testing with QFT-IT) will be economical and help in treating LTBI cases in lower middle income countries like India.
SOX4 is a direct target gene of FRA-2 and induces expression of HDAC8 in adult T-cell leukemia/lymphoma
Authors: Higuchi Tomonori , Nakayama Takashi , Arao Tokuzo , Nishio Kazuto , Yoshie Osamu 
Published: March 2013

Key PointsSOX4 is consistently expressed in ATL, is involved in ATL cell growth, and induces genes such as GCRK, NAP1, and HDAC8 in ATL. FRA-2/JUND and SOX4 form an important oncogenic cascade in ATL, leading to upregulation of genes such as HDAC8.
Autologous/reduced-intensity allogeneic stem cell transplantation vs autologous transplantation in multiple myeloma: long-term results of the EBMT-NMAM2000 study
Authors: Gahrton Gösta , Iacobelli Simona , Björkstrand Bo , Hegenbart Ute , Gruber Astrid , Greinix Hildegard , Volin Liisa , Narni Franco , Carella Angelo Michele , Beksac Meral , Bosi Alberto , Milone Giuseppe , Corradini Paolo , Schönland Stefan , Friberg Kristina , van Biezen Anja , Goldschmidt Hartmut , de Witte Theo , Morris Curly , Niederwieser Dietger , Garderet Laurent , Kröger Nicolaus 
Published: March 2013

Key Points
Tandem autologous/reduced-intensity allogeneic transplantation is superior to autologous transplantation alone in multiple myeloma.
A note on the "Equation missing" -zeta-type function with weight α
Authors: Cetin Elif , Acikgoz Mehmet , Cangul Ismail Naci , Araci Serkan 
Published: March 2013

Abstract
The objective of this paper is to derive the symmetric property of an

(
h
,
q
)

-zeta function with weight α. By using this property, we give some interesting identities for

(
h
,
q
)

-Genocchi polynomials with weight α. As a result, our applications possess a number of interesting properties which we state in this paper.

MSC:11S80, 11B68.
Characterization of expressed sequence tags from developing fibers of Gossypium barbadenseand evaluation of insertion-deletion variation in tetraploid cultivated cotton species
Authors: Lv Yuanda , Zhao Liang , Xu Xiaoyang , Wang Lei , Wang Cheng , Zhang Tianzhen , Guo Wangzhen 
Published: March 2013

Abstract

Background
Cotton is the leading fiber crop worldwide. Gossypium barbadense is an important species of cotton because of its extra-long staple fibers with superior luster and silkiness. However, a systematic analysis and utilization of cDNA sequences from G. barbadense fiber development remains understudied.

Results
A total of 21,079 high quality sequences were generated from two non-normalized cDNA libraries prepared by using a mixture of G. barbadense Hai7124 fibers and ovules. After assembly processing, a set of 8,653 unigenes were obtained. Of those, 7,786 were matched to known proteins and 7,316 were assigned to functional categories. The molecular functions of these unigenes were mostly related to binding and catalytic activity, and carbohydrate, amino acid, and energy metabolisms were major contributors among the subsets of metabolism. Sequences comparison between G. barbadense and G. hirsutum revealed that 8,245 unigenes from G. barbadense were detected the similarity with those released publicly in G. hirsutum, however, the remaining 408 sequences had no hits against G. hirsutum unigenes database. Furthermore, 13,275 putative ESTs InDels loci involved in the orthologous and/or homoeologous differences between/within G. barbadense and G. hirsutum were discovered by in silico analyses, and 2,160 InDel markers were developed by ESTs with more than five insertions or deletions. By gel electrophoresis combined with sequencing verification, 71.11% candidate InDel loci were reconfirmed orthologous and/or homoeologous loci polymorphisms using G. hirsutum acc TM-1 and G. barbadense cv Hai7124. Blastx result showed among 2,160 InDel loci, 81 with significant function similarity with known genes associated with secondary wall synthesis process, indicating the important roles in fiber quality in tetraploid cultivated cotton species.

Conclusion
Sequence comparisons and InDel markers development will lay the groundwork for promoting the identification of genes related to superior agronomic traits, genetic differentiation and comparative genomic studies between G. hirsutum and G. barbadense.
Transcriptomics of morphological color change in polychromatic Midas cichlids
Authors: Henning Frederico , Jones Julia C , Franchini Paolo , Meyer Axel 
Published: March 2013

Abstract

Background
Animal pigmentation has received much attention in evolutionary biology research due to its strong implications for adaptation and speciation. However, apart from a few cases the genetic changes associated with these evolutionary processes remain largely unknown. The Midas cichlid fish from Central America are an ideal model system for investigating pigmentation traits that may also play a role in speciation. Most Midas cichlids maintain their melanophores and exhibit a grayish (normal) color pattern throughout their lives. A minority of individuals, however, undergo color change and exhibit a distinctive gold or even white coloration in adulthood. The ontogenetic color change in the Midas cichlids may also shed light on the molecular mechanisms underlying pigmentation disorders in humans.

Results
Here we use next-generation sequencing (Illumina) RNAseq analyses to compare skin transcriptome-wide expression levels in three distinct stages of color transformation in Midas cichlids. cDNA libraries of scale tissue, for six biological replicates of each group, were generated and sequenced using Illumina technology. Using a combination of three differential expression (DE) analyses we identified 46 candidate genes that showed DE between the color morphs. We find evidence for two key DE patterns: a) genes involved in melanosomal pathways are up-regulated in normally pigmented fish; and b) immediate early and inflammatory response genes were up-regulated in transitional fish, a response that parallels some human skin disorders such as melanoma formation and psoriasis. One of the DE genes segregates with the gold phenotype in a genetic cross and might be associated with incipient speciation in this highly “species-rich” lineage of cichlids.

Conclusions
Using transcriptomic analyses we successfully identified key expression differences between different color morphs of Midas cichlid fish. These differentially expressed genes have important implications for our understanding of the molecular mechanisms underlying speciation in this lineage of extremely young species since they mate strongly assortatively, and new species may arise by sexual selection due to this color polymorphism. Some of the human orthologues of the genes identified here may also be involved in pigmentation differences and diseases and therefore provide genetic markers for the detection of human pigmentation disorders.
Recognition of face-name associations after errorless and errorful learning: an fMRI study
Authors: Hammer Anke , Tempelmann Claus , Münte Thomas F 
Published: March 2013

Abstract

Background
Errorless learning has advantages over errorful learning. The erroneous items produced during errorful learning compete with correct items at retrieval resulting in decreased memory performance. This interference is associated with an increased demand on executive monitoring processes. Event-related functional magnetic resonance imaging (fMRI) was used to contrast errorless and errorful learning. Learning mode was manipulated by the number of distractors during learning of face-name associations: in errorless learning only the correct name was introduced. During errorful learning either one incorrect name or two incorrect names were additionally introduced in order to modulate the interference in recognition.

Results
The behavioural results showed an enhanced memory performance after errorless learning. The veridicality of recognition of the face-name associations was reflected in a left lateralized fronto-temporal-parietal network. The different learning modes were associated with modulations in left prefrontal and parietal regions.

Conclusions
Errorless learning enhances memory performance as compared to errorful learning and underpins the known advantages for errorless learning. During memory retrieval different networks are engaged for specific purposes: Recognition of face-name associations engaged a lateralized fronto-temporal-parietal network and executive monitoring processes of memory engaged the left prefrontal and parietal regions.
Beclin1 inhibition promotes autophagy and decreases gemcitabine–induced apoptosis in Miapaca2 pancreatic cancer cells
Authors: Li Xiaoshu , Yan Jun , Wang Lisheng , Xiao Fengjun , Yang Yuefeng , Guo Xiaozhong , Wang Hua 
Published: March 2013

Abstract

Background
Beclin1 is a well-known key regulator of autophagy, which is also a haploinsufficient tumor suppressor. Current studies revealed that down-regulation or monoallelic deletions of Beclin1 were frequently found in various cancers. The purpose of this study was to investigate the effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of pancreatic cancer cells.

Methods
Beclin1 expression was inhibited by siRNA transduction and gene expression was determined by Real-time PCR and Western blot. The effects of Beclin1 inhibition on autophagy and Gemcitabine-induced apoptosis of Miapaca2 cells were analyed through LC3 expression, cell viability, cell cycle and apoptosis by using Western blot.

Results
We observed that Beclin1 silence promoted microtubule-associated protein 1 light chain 3-II (LC3-II) protein formation and increased punctate fluorescent signals in Miapaca2 cells transfected with green fluorescent protein (GFP)-tagged LC3. Beclin1 inhibition showed a greater suppressive effect on Gemcitabine-induced apoptosis of Miapaca2 cells.

Conclusion
Our data suggested that Beclin1 silence not only up-adjusted autophagy process, but also played an important role in the regulation of apoptosis. Beclin1 inhibition could inhibit apoptosis signaling induced by Gemcitabine in Miapaca2 cells.
Synchronous triple carcinoma of the colon and rectum
Authors: Yeh Chien-Chih , Hsi Sheng-Chuan , Chuu Chih-Pin , Kao Yung-Hsi 
Published: March 2013

Abstract
Synchronous multiple colorectal cancers are defined as multiple malignant colorectal tumors that occur simultaneously. All tumors are distant from each other, and none are the result of metastasis from other tumors. Here, we present a case of a 79-year-old man who was admitted to our hospital because of a 3-month history of abdominal pain associated with anemia, loss of appetite, and body weight loss. The patient did not have a family history of cancer. Computed tomography revealed bowel wall thickness and mesentery inflammation at the hepatic flexure of the colon and cecum. Colonoscopy revealed a tumor located 10 cm from the anal verge. Colonoscopic examination of the large bowel was not possible because of bowel obstruction due to the rectal tumor. Synchronous triple adenocarcinoma of the colon and rectum was confirmed by pathologic examination. The tumor was surgically resected by two-segment resection of the colon, low anterior resection, and right hemicolectomy. We used intraoperative colonoscopy to confirm that there were no other lesions after the resection of the three tumors. To the best of our knowledge, this is the first case of synchronous triple carcinoma of the colon and rectum in Taiwan. We consider that comprehensive preoperative study, extensive intraoperative exploration, and radical resection can increase the survival rate of patients with synchronous multiple colorectal cancers.