bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study on safety health of construction workers at workplace: a sustainable perspective approach
Authors: Kumar Singh Atul , Aljohani Abdulelah , Shakor Pshtiwan , Awuzie Bankole Osita , Uddin S. M. Jamil , Shivendra B. T. 
Published: August 2024

In the construction industry, irregular schedules and long hours disrupt workers’ circadian rhythms, adversely affecting their safety and health. While studies have shown that sleep timing influences safety and health, they often overlook sleep quantity, work-shift start time, and a broad range of safety and health outcomes. To fill this gap, this study examines the relationship between circadian rhythm and safety health factors, including psychological issues, wellbeing, behavioral issues, altruistic behaviors, sleep quantity, starting hours at work, and parental care in 1,400 construction workers using a questionnaire. We investigated safety health using scales for psychological issues (0–34), wellbeing (0–24), behavioral issues (0–29), and altruistic behaviors (0–27). Midsleep time on weekends estimated circadian rhythm, with multilevel regressions adjusting for sleep quantity, work start time, and various characteristics. Results showed average midsleep at 04:11 h, with a 1 h delay associated with increased psychological issues (0.37), behavioral issues (3.0%), decreased wellbeing (0.19), and decreased altruistic behaviors (0.18). These findings highlight a correlation between early circadian rhythm and poor safety health, independent of sleep quantity and work-shift start time. Addressing circadian rhythm disruptions could be crucial for improving safety health outcomes, suggesting that work schedule adjustments and better sleep practices may mitigate adverse effects on workers’ health and wellbeing.
Construction and optimisation of watershed scale ecological network: a case study of kuye river basin
Authors: Wu Yihan , Qin Fucang , Li Long , Dong Xiaoyu 
Published: August 2024

IntroductionCreating an ecological space network is essential for safeguarding the core structure of ecological space.MethodsMorphological spatial pattern analysis was used to locate ecological sources in the Kuye River Basin. Using the least cumulative resistance model and gravity model, the resistance surface, ecological corridor, and ecological space management network are determined.Results and discussionThe study revealed that in 2022, the predominant land use types in the Kuye River Basin were wood land and grassland, cultivated land, and construction land. MSPA model software identifies a substantial portion of the landscape pattern as consisting of core and marginal areas, which encompass 30324.05 hm2 and 15088.24 hm2. High ecological resistance surface factors dominate the socioeconomically vibrant zone and northern regions. Resistance values ranging from 0.02 to 0.87, and high and law resistance zones alternate. The minimal cumulative resistance approach found 171 ecological corridors. And gravity model using the interaction matrix of 19 primary ecological sources discovered 8 first-level ecological corridors with the highest interaction force. There are 137 core and 23 subsidiary ecological corridors with significant affects. Overlying the road factors and ecological corridors of national highways, provincial roads, railways, and high-speed roads creates a total of 38 ecological breakpoints, each characterized by specific barrier effects and legal ecological stability.
Spatial characteristics and optimization of urban living space carbon suitability index (ULS-CSI) in Tianjin, China
Authors: Yin Zhaowei , Zhang Xiaoping , Chen Peng , Liao Qinghua 
Published: August 2024

The global climate crisis is escalating, and urban living Space (ULS) is a significant contributor to carbon emissions. How to improve the carbon suitability of ULS while promoting social and economic development is a global issue. This study aims to develop an evaluation system for comparing and analyzing carbon suitability inequality and spatial differences in different areas. To achieve this goal, an urban living space carbon suitability index (ULS-CSI) based on spatial organizational index (SOI) has been proposed. The ULS-CSI was calculated at the area scale in Tianjin using information from the Tianjin Land Use Database in 2021. The carbon emissions coefficient method was used to calculate the urban living space carbon emissions (ULSCE). Moran’I and LISA analysis were used to quantify the spatial differences of ULS-CSI. The results showed that the residential living area (RLA) carbon emissions was the highest at the area scale, with carbon emissions of 1.14 × 1011 kg, accounting for 33.74%. The green space leisure area (GLA) carbon absorption was the highest at the area scale, with carbon absorption of 5.76 × 105 kg, accounting for 32.33%. SOI in different areas have spatial heterogeneity as the SOI such as building area, road network density and land use characteristics are significantly different in different areas. Areas with superior CSI were primarily situated in Heping, Hexi, Nankai, and Beichen, accounting for 83.90%. Conversely, areas under the basic CSI threshold included Xiqing, Jinnan, and Dongli, accounting for 16.10%. Spatial characteristics of ULS-CSI in Tianjin portrayed a significant spatial positive correlation, indicating the highest autocorrelation degree of CSI at 500 m, with a Moran ’I value of 0.1733. Although these findings reflect the spatial characteristics of ULS-CSI and the SOI affecting the ULS-CSI at area scale, more perfect data are needed to reflect the complexity of structural factors affecting ULS-CSI at area scale. This study is helpful for urban planning to develop differentiated carbon reduction strategies and promote low-carbon and healthy urban development.
Transcriptome analysis of four types of gonadal tissues in largemouth bass (Micropterus salmoides) to reveal its sex-related genes
Authors: Zhang Dongyun , Tian Taihang , Li Shengjie , Du Jinxing , Lei Caixia , Zhu Tao , Han Linqiang , Song Hongmei 
Published: August 2024

The sex determination system of largemouth bass (Micropterus salmoides, LMB) is XX/XY; however, the underlying molecular mechanisms involved in early sex differentiation, gonadal development, and exogenous hormone-induced sex reversal remain unknown. In this study, LMB at 15 days post-hatching (dph) were fed diets containing 20 mg/kg of 17α-methyltestosterone (17α-MT) or 30 mg/kg of 17β-estradiol (17β-E2) for 60 days, respectively. Serum steroid levels, histological observations of the gonads, and identification of sex-specific markers were employed to screen the gonads of 60-day-old normal female fish (XX-F), normal male fish (XY-M), 17β-E2 induced pseudo-female fish (XY-F), and 17α-MT-induced pseudo-male fish (XX-M) for transcriptome sequencing in order to uncover genes and pathway involved in the process of sexual reversal. The results from histology and serum sex steroid hormone analysis showed that both 17α-MT and 17β-E2 were capable of inducing sex reversal of LMB at 15 dph. Transcriptome results revealed a total of 2,753 genes exhibiting differential expression, and the expression pattern of these genes in the gonads of XX-M or XY-F resembled that of normal females or males. The male sex-biased genes that are upregulated in XX-M and downregulated in XY-F are referred to as key genes for male reversal, while the female sex-biased genes that are upregulated in XY-F and downregulated in XX-M are referred to as key genes for female reversal. Finally, 12 differentially expressed genes (DEGs) related to male sex reversal were screened, including star2, cyp17a, cyp11b1, dmrt1, amh, sox9a, katnal1, spata4, spata6l, spata7, spata18 and foxl3. 2 DEGs (foxl2a and cyp19a1b) were found to be associated with female sex reversal. The changes in these genes collectively influence the direction of sex differentiation of LMB. Among them, star2, dmrt1 and cyp19a1b with significantly altered expression levels may play potentially crucial role in the process of gender reversal. The expression patterns of 21 randomly selected genes were verified using qRT-PCR which confirmed the reliability and accuracy of the RNA-seq results. These findings not only enhance our understanding of the molecular basis underlying sex reversal but also provide crucial data support for future breeding research on unisexual LMB.
Role of circulating microparticles and cytokines in periodontitis associated with diabetes
Authors: Cassiano Bárbara Adelungue , Silveira Ana Luíza Pereira Assunção , Kim Yeon Jung , do Amaral Jônatas Bussador , da Silva Nali Luiz Henrique , Bachi André Luis Lacerda , Resende Leonardo Diniz , Fonseca Francisco Antonio Helfenstein , de Oliveira Izar Maria Cristina , Tuleta Izabela Dorota , Victor Jefferson Russo , Pallos Débora , França Carolina Nunes 
Published: August 2024

BackgroundPeriodontitis is a chronic inflammatory condition that affects the supporting tissues of the teeth, and can lead to serious complications such as tooth loss and systemic health problems, including diabetes, which have a bidirectional relationship with periodontitis. Circulating microparticles originate from different cell types after stimuli such as activation or apoptosis. Interleukins are related to processes in the regulation of the immune response, inflammation, and cell growth. This study aimed to evaluate circulating microparticles as well as interleukins in the plasma, at baseline and 1 month after the end of the non-surgical periodontal treatment.MethodsSamples were collected from 45 patients, with moderate to severe periodontitis with diabetes (N = 25) and without diabetes (N = 20). Microparticles were evaluated in the platelet-poor plasma by flow cytometer. Cytokine levels were evaluated by the enzyme immunoabsorption assay (ELISA).ResultsHigher levels of the pro-inflammatory cytokines were found in the group with diabetes compared to the non-diabetic group both at baseline and 1 month after the end of the treatment. A higher IL-6/IL-10 ratio was found in patients with diabetes compared to the group without diabetes at T0 and T1, whereas an increased IFN-γ/IL-10 ratio was only found at T1 in patients with diabetes in comparison to the group without diabetes. In the group with diabetes, it was verified positive correlations between IL-10 and IL-6 or IFN-γ and a negative correlation between IL-6 and PMP, at T0; in contrast, in the T1, negative correlations were found between TNF-α and IL-10 or PMP. Besides, at T0, it was evidenced positive correlations both between circulating TNF-α and IL-6, and IL-10 and EMP, as well as a negative correlation between IL-10 and PMP in the group with diabetes. In addition, it was observed in T1 positive correlations between levels of TNF-α and IL-6, IFN-γ, or IL-10, and between PMP and IFN-γ, and between EMP and IL-6, TNF-α and IFN-γ in this group.ConclusionThe results suggest a modulatory effect of the periodontitis associated with diabetes, as well as the periodontal treatment, in the systemic inflammatory status of the participants of the study.
Unveiling the hidden link: fungi and HPV in cervical lesions
Authors: Zhang Yulong , Chen Lingsi , Li Haibo , Zhuang Yiling , Xie Qing , Li Wenwen , Yang Xia , Zheng Xiangqin , Suyu Li , Yi Huan 
Published: August 2024

BackgroundCervical cancer, primarily driven by high-risk human papillomavirus (HR-HPV) infection, ranks as the second most common cancer globally. Understanding combined infections’ role, including Cervical fungi, is crucial in cervical carcinogenesis. This study aims to explore the potential correlation between HR-HPV, cervical fungi, and cervical cancer, while adjusting for various factors.MethodsThe study population comprised patients undergoing colposcopy and conization due to abnormal cervical screening results. Clinical data including age, gravidity, HPV (human papillomavirus) genotypes, cervical pathology, and p16/Ki67 expression were extracted. Cervical TCT (ThinPrep Pap Test) and HPV testing are utilized for screening cervical lesions, with fungal presence suggested by TCT results. 5,528 participants were included in this study. Statistical analyses investigated associations between HPV/fungi co-infection and cervical lesions, employing multinomial logistic regression and interaction analysis.ResultsCo-infection with fungi and HPV may decrease the risk of cervical lesions compared to HPV infection alone. In the co-infection group, compared with HPV infection alone, the risk of low-grade squamous intraepithelial lesions (LSIL) was reduced by 27% (OR = 0.73, 95% CI: 0.59–0.90), the risk of high-grade squamous intraepithelial lesions (HSIL) was reduced by 35% (OR = 0.65, 95% CI: 0.51–0.82), and the risk of cervical cancer was reduced by 43% (OR = 0.57, 95% CI: 0.35–0.92). The interaction analysis revealed a negative interaction between fungal and HPV infections in the development of cervical cancer (RERI = −6.25, AP = −0.79, SI = 0.52), HSIL (RERI = −19.15, AP = −0.37, SI = 0.72) and LSIL (RERI = −1.87, AP = −0.33, SI = 0.71), suggesting a sub-additive effect, where the combined effect of the two infections was less than the sum of their individual effects. This indicates that fungal infection may attenuate the promoting effect of HPV on cervical lesions. In exploring the potential mechanism, we found that the co-infection group had significantly lower p16 positivity (54.6%) compared to the HPV-only group (60.2%) (p = 0.004), while there was no statistically significant difference in Ki67 positivity.ConclusionThis study unveils the intricate relationship between cervical fungi and HPV in cervical lesions. Co-infection with fungi and HPV against cervical lesions compared to HPV infection alone, indicating a novel clinical interaction. Lower p16 positivity in co-infection hints at a protective mechanism, urging further exploration.
Nitrogen uptake, grain yield, and oil concentration of dwarf castor beans under nitrogen rates and inoculation of rhizobacteria in grasses–legumes rotation
Authors: Gato Isabela Martins Bueno , Oliveira Carlos Eduardo da Silva , Jalal Arshad , Moreira Vitória de Almeida , Hashem Amr H. , de Lima Bruno Horschut , Leite Gabriel da Silva , Al-Askar Abdulaziz A. , Freitas Leandro Alves , AbdElgawad Hamada , Ferreira Selton Vinicius Domingos , Santana Leticia de Jesus , Bastos Andréa de Castro , Galindo Fernando Shintate , Zoz Tiago , Teixeira Filho Marcelo Carvalho Minhoto 
Published: August 2024

IntroductionPlant growth-promoting bacteria (PGPB) have been primarily studied for atmospheric nitrogen (N) fixation but they also have the capacity to improve nutrition and yield of crop plants.MethodsTherefore, the objective of this research was to investigate the effects of inoculation with PGPB in association with different N rates on N uptake, grain yield, and oil concentration of dwarf castor beans in succession to legumes and grasses in Ilha Solteira, Brazil. The treatments consisted of N rates (0 to 180 kg ha−1 of N) and inoculation with three plant growth-promoting bacteria (Azospirillum brasiliense, Bacillus subtilis, and Pseudomonas fluorescens, applied by leaf) and a control with no-inoculation.ResultsThe grain and oil yields of castor beans were increased by 20 and 40% at a rate of 103 kg ha−1 of N in succession to grasses as compared to without N application. In addition, the grain yield of castor bean after legumes was increased by 28, 64, and 40% with estimated rates of 97, 113, and 92 kg ha−1 of N in combination with inoculations of A. brasilense, B. subtilis, and P. fluorescens as compared to without N application, respectively. Shoot, grain, and total N uptake were improved with foliar inoculation of A. brasilense, B. subtilis, and P. fluorescens at the N rates of 45, 90, and 135 kg ha−1, respectively.Discussion and conclusionsTopdressing of N at the rate of 103 kg ha−1 and foliar inoculation in succession to grasses and 180 kg ha−1 of N without the effect of foliar inoculation in succession to legumes are recommended for higher grain and oil yield of castor beans. Foliar inoculations with A. brasilense, B. subtilis, and P. fluorescens increased grain yield under reduced use of N fertilizer by 44, 37, and 49% in dwarf castor cultivation in succession to legumes, potentially contributing to sustainable agriculture.
Digital Clock and Recall: a digital, process-driven evolution of the Mini-Cog
Authors: Gomes-Osman Joyce , Borson Soo , Toro-Serey Claudio , Banks Russell , Ciesla Marissa , Jannati Ali , Morrow W. Isaiah , Swenson Rod , Libon David , Bates David , Showalter John , Tobyne Sean , Pascual-Leone Alvaro 
Published: August 2024

IntroductionAlzheimer’s disease and related dementias (ADRD) represent a substantial global public health challenge with multifaceted impacts on individuals, families, and healthcare systems. Brief cognitive screening tools such as the Mini-Cog© can help improve recognition of ADRD in clinical practice, but widespread adoption continues to lag. We compared the Digital Clock and Recall (DCR), a next-generation process-driven adaptation of the Mini-Cog, with the original paper-and-pencil version in a well-characterized clinical trial sample.MethodsDCR was administered to 828 participants in the Bio-Hermes-001 clinical trial (age median ± SD = 72 ± 6.7, IQR = 11; 58% female) independently classified as cognitively unimpaired (n = 364) or as having mild cognitive impairment (MCI, n = 274) or dementia likely due to AD (DLAD, n = 190). MCI and DLAD cohorts were combined into a single impaired group for analysis. Two experienced neuropsychologists rated verbal recall accuracy and digitally drawn clocks using the original Mini-Cog scoring rules. Inter-rater reliability of Mini-Cog scores was computed for a subset of the data (n = 508) and concordance between Mini-Cog rule-based and DCR scoring was calculated.ResultsInter-rater reliability of Mini-Cog scoring was good to excellent, but Rater 2’s scores were significantly higher than Rater 1’s due to variation in clock scores (p &lt; 0.0001). Mini-Cog and DCR scores were significantly correlated (τB = 0.71, p &lt; 0.0001). However, using a Mini-Cog cut score of 4, the DCR identified more cases of cognitive impairment (n = 47; χ2 = 13.26, p &lt; 0.0005) and Mini-Cog missed significantly more cases of cognitive impairment (n = 87). In addition, the DCR correctly classified significantly more cognitively impaired cases missed by the Mini-Cog (n = 44) than vice versa (n = 4; χ2 = 21.69, p &lt; 0.0001).DiscussionOur findings demonstrate higher sensitivity of the DCR, an automated, process-driven, and process-based digital adaptation of the Mini-Cog. Digital metrics capture clock drawing dynamics and increase detection of diagnosed cognitive impairment in a clinical trial cohort of older individuals.
The AST/ALT ratio predicts survival and improves oncological therapy decisions in patients with non-small cell lung cancer receiving immunotherapy with or without radiotherapy
Authors: Zhang Yanyan , Zhang Jingxin , Shang Shijie , Ma Jiachun , Wang Fei , Wu Meng , Yu Jinming , Chen Dawei 
Published: August 2024

Background and purposeImmunotherapy, with or without radiotherapy (iRT or ICIs-nonRT), is the standard treatment for non–small cell lung cancer (NSCLC). Nonetheless, the response to the treatment varies among patients. Given the established role of aspartate aminotransferase/alanine transaminase (AST/ALT) ratio in predicting cancer prognosis, we sought to identify whether the pre-treatment AST/ALT ratio has the potential to serve as a prognostic factor for NSCLC patients receiving ICIs-nonRT and iRT.Materials and methodsWe retrospectively analyzed NSCLC patients who received immunotherapy between April 2018 and March 2021. Patients were classified into iRT group and ICIs-nonRT group and further classified based on AST/ALT ratio cut-off values. The Kaplan-Meier (KM) method estimated the time-to-event endpoints (progression-free survival (PFS) and overall survival (OS)ResultsOf the cohort, 239 underwent ICIs-nonRT and 155 received iRT. Higher AST/ALT ratios correlated with worse outcomes in the ICIs-nonRT group but indicated better outcomes in those who received iRT. Multivariate analysis validated AST/ALT ratio as an independent prognostic factor. For AST/ALT ratios between 0.67-1.7, both ICIs-nonRT and iRT yielded similar treatment outcomes; with AST/ALT ratios greater than 1.7, iRT could be a more favorable treatment option (P=0.038). Conversely, for ratios less than 0.67, ICIs-nonRT could be a more favorable treatment option (P=0.073).ConclusionsThe pre-treatment AST/ALT ratio demonstrates potential as a prognostic marker for treatment outcomes in NSCLC patients receiving either ICIs-nonRT or iRT. This finding could help guide clinicians in selecting more effective treatment protocols, thereby enhancing patient prognosis.
Clinical utility of repeated rebiopsy for EGFR T790M mutation detection in non-small cell lung cancer
Authors: Lee Eun Hye , Kwak Se Hyun , Kim Kyeong Yeon , Kim Chi Young , Lee Sang Hoon , Heo Seok-Jae , Chang Yoon Soo , Kim Eun Young 
Published: August 2024

PurposeIn cases where rebiopsy fails to find the epidermal growth factor receptor (EGFR) T790M mutation, the criteria for selecting patients for repeated rebiopsy remains unclear. This study aimed to assess the impact of repeated rebiopsy on T790M mutation detection in non-small cell lung cancer (NSCLC) patients.MethodsPatients with advanced EGFR-mutated NSCLC between January 2018 and December 2021 at three-referral hospitals in South Korea underwent retrospective review. Of 682 patients who had rebiopsy after disease progression, T790M mutation status was assessed in plasma circulating tumor DNA (ctDNA) and/or tumor tissues.ResultsThe overall T790M positivity rate increased from 40.8% after the first rebiopsy to 52.9% following multiple rebiopsies in the entire study population. Longer duration of initial EGFR TKI use (OR 1.792, ≥8 months vs. &lt;8 months, p=0.004), better EGFR TKI responses (OR 1.611, complete or partial response vs. stable disease, p=0.006), presence of bone metastasis (OR 2.286, p&lt;0.001) were correlated with higher T790M positivity. Longer EGFR TKI use and better responses increased T790M positivity in repeated tissue rebiopsy, while bone metastasis favored liquid rebiopsy. Additionally, T790M status has been shown to be positive over time through repeated rebiopsies ranging from several months to years, suggesting its dynamic nature.ConclusionIn this study, among patients who initially tested negative for T790M in rebiopsy, repeated rebiopsies uncovered an additional 23.5% T790M positivity. Particularly, it is suggested that repeated rebiopsies may be valuable for patients with prolonged EGFR TKI usage, better responses to treatment, and bone metastasis.
Relaxing at work: does virtual reality work? An exploratory study among employees in their workplace
Authors: Michinov Estelle , Dodeler Virginie , Adam Hugo , Michinov Nicolas 
Published: August 2024

IntroductionThe development of virtual reality provides new opportunities for conducting relaxing activities in a simulated environment. Although studies on the effects of VR-based relaxation are developing, they have mostly been performed with students, and more rarely with employees.MethodsThis study aimed to examine the effects of VR relaxation exercises with employees. Forty-seven employees were divided into two conditions: Virtual Reality (n = 25) and 2D video (n = 22).ResultsThe results indicate that relaxing activities reduced stress and negative emotions, while increasing perceived restoration using VR and 2D video. The acceptability and user experience were higher only in the VR condition. VR relaxation provides a sense of disconnection from the workplace that allows employees to increase the pleasant experience.DiscussionThe 2D solution, while less immersive, can be an alternative for employees for whom VR is not recommended. The question of inclusive digital tools is a key issue in the quality of working life programs.