bluuebunny/crossref_metadata_2025_split huggingface dataset:
DEMONSTRATION OF DNP GROUPS ON IN VITRO DINITROPHENYLATED LYMPH NODE CELLS OF THE GUINEA PIG BY A SCANNING IMMUNOELECTRON MICROSCOPIC METHOD
Authors: Nakaye Masako , Nakagawa Shojiro , Mimura Shigeru , Uekt Hiroaki , Kumon Hiromi , Ohmori Hiroyuki 
Published: April 2015

ABSTRACTThe distribution of DNP groups on the surface of guinea pig lymph node cells incubated with 2.5 mM DNBSO3Na in tubes was investigated by an indirect method using scanning immunoelectron microscopy with bacteriophage T4 labelled with antibody as a visual marker. Fairly precise localization could be achieved with scanning electron microscopy. It became apparent that DNP groups were distributed in varying amounts on the surface of lymph node cells incubated with DNBSO3Na in tubes and that DNP groups had a tendency to be distributed patchily on cells sufficiently exposed. The frequency of the cells to which DNP groups attached was approximately 50%. The significance of these findings is discussed.
A CASE OF DIFFUSE PLANE XANTHOMATOSIS
Authors: Yu Hsin‐Su , Ishibashi Yasumasa , Kukita Atsushi 
Published: April 2015

ABSTRACTA 54 year old woman had had typical symptoms of diffuse plane xanthomatosis of 9 years' duration about the eyelids, neck, trunk and extremities. Episodes of intermittent attacks of edema and erythema in the xanthomatous plaques were noticed. Serum complement examination revealed low levels of CH50, C1q, C4, C2 and C1 esterase inhibitor, but C3 was within normal limits. These results are similar to those found in hereditary angio‐neurotic edema. The angio‐edema like symptoms in this case are discussed in the light with this abnormal complement component profile.
X‐LINKED ICHTHYOSIS
Authors: Saita Buzou , Kikuchi Ichiro , Hiejima Mutsusuke , Inoue Shohei , Fukuzaki Masashi 
Published: April 2015

ABSTRACTWe report 5 families with X‐linked ichthyosis, of whom 14 individuals were examined. The onset was between 1 month and 3 months after birth in seven cases out of 11. Ichthyosis was marked on the head, ears, abdomen and limbs. In some cases, the antecubital, popliteal and axillary fossae were affected. Dark brown scales, characteristic of X‐linked ichthyosis, were observed in 11 cases out of 14. Histological examination revealed 3 to 5 layers of the granular layer in all of the nine cases examined. Ophthalmological slit lamp examination revealed bilateral deep corneal opacities in 5 cases out of 14 cases of X‐linked ichthyosis. Punctate corneal clouding was observed in two out of 10 identified carriers of X‐linked ichthyosis.All 24 children born of 7 cases of X‐linked ichthyosis were not affected. All parents of patients with X‐linked ichthyosis were also unaffected. Ichthyosis was observed in 26 male children out of 44 male children born of 21 carriers of X‐linked ichthyosis. These data attest to the X‐linked recessive mode of inheritance.
PRURIGO NODULARIS A POSSIBLE RELATIONSHIP TO ATOPY
Authors: Miyachi Yoshiki , Okamoto Hiroyuki , Furukawa Fukumi , Imamura Sadao 
Published: April 2015

ABSTRACTThe possible relationship between prurigo nodularis and atopy was studied revealing that prurigo nodularis occurs more readily in atopics. Our results suggest that some prurigo nodularis is associated with an atopic background in pathogenesis. We speculate that patients with atopy are more sensitive to insect bites or other scratch‐provoking triggers which often leads to intensive scratching resulting in the verrucous appearance of the eruption.
THE HYDROXYL RADICAL IS NOT INVOLVED WITH TYROSINASE INACTIVATION
Authors: Tomita Yasushi , Seiji Makoto , Igarashi Akira 
Published: April 2015

ABSTRACTTyrosinase [EC 1.4.18.1] in the mouse melanoma melanosome was inactivated during the dopatyrosinase reaction. When the air phase in the reaction mixture was replaced by N2 gas, tyrosinase inactivation during incubation with dopa did not occur. This may indicate that oxygen radicals and/or hydroxyl radicals (OH) are generated in the tyrosinase reaction and attack tyrosinase itself with inactivation. As shown in our previous report, oxygen radicals such as superoxide anion (O2−) and singlet oxygen (1O2) have nothing to do with tyrosinase inactivation. In this paper, we tested whether or not the hydroxyl radical could be related to the inactivation of tyrosinase. The enzyme inactivation was not prevented by D‐mannitol, a scavenger for hydroxyl radical. Furthermore, production of hydroxyl radicals which react spontaneously with methionine to yield ethylene, which can be measured by gas chromatography, was not detected in this enzyme reaction. Thus the inactivation involved is not due to the hydroxyl radical.
A CASE OF VITILIGO VULGARIS WITH IgG DETECTED AT THE DERMOEPIDERMAL JUNCTION—WITH SPECIAL REFERENCE TO ITS TREATMENT WITH OK‐432
Authors: Sawada Yoko , Ohashi Masaru 
Published: April 2015

ABSTRACTIn a case of vitiligo vulgaris, IgG was detected at the dermoepidermal junction in the involved region of the face using the direct fluorescent‐antibody technique. In this case there was also an area of rippled pigmentation of the skin on the back, where amyloid deposition was demonstrated by staining with thioflavine T. No amyloid was present in the vitiliginous area of the face.The disease failed to respond to Methoxalen but healed almost completely as a result of intradermal injections of OK‐432. OK‐432 appears to provide a practical and theoretically sound method of treatment for the disease.
IMMUNE COMPLEXES IN THE SERA OF PATIENTS WITH ANTI‐RNP ANTIBODIES
Authors: Miyagawa Sachiko , Sakamoto Kuniki 
Published: April 2015

ABSTRACTCirculating immune complexes were detected by the polyethylene glycol precipitation method in the sera of patients with antibodies to ribonucleoprotein (RNP). Levels of immune complexes were significantly higher than in normal control sera and correlated well with disease activity as defined by clinical manifestations, decreased levels of complement and increased fluorescent anti‐nuclear antibody titers.Analysis of IgG‐aggregates in the sera of patients revealed the following characteristics: molecular weight of about 900,000 daltons, sensitivity to RNase, resistance to DNase, no Clq‐binding activity and no RF activity.
IMMUNOLOGICAL STUDIES OF ONCHOCERCIASIS IN GUATEMALA
Authors: Akiyama Tomio , Ushijima Nobuo , Anan Sadao , Nonaka Shigeo , Yoshida Hikotaro , Flores Guillermo Efrain Zea 
Published: April 2015

ABSTRACTThe serum levels of the four immunoglobulins, IgG, IgA, IgM and IgE, in Guatemalan onchocerciasis patients were determined quantitatively using a laser immunoassay system or radioimmunosorbent test.The IgG and IgE levels in Guatemalan onchocerciasis patients positive for Onchocerca volvulus microfilariae were significantly higher than the levels in Guatemalan controls negative for microfilariae and in healthy Japanese controls. But, no differences in the levels of IgA and IgM were obtained between the Guatemalan onchocerciasis patient group and the two control groups.
SPONTANEOUS REGRESSION OF HUMAN MALIGNANT MELANOMA
Authors: Hori Yoshiaki , Masuzawa Mikio , Takezaki Shinichiro , Arai Harue 
Published: April 2015

ABSTRACTCellular and subcellular changes and cell‐mediated immunity of a patient (a 26‐year‐old man) with spontaneously regressed malignant melanoma are described.The patient developed black nodules on his left leg and foot several times over a period of 11 years. These nodules necrotized, regressed to form crusted nodules and disappeared leaving white macules.Many melanin‐laden cells (so‐called melanophages) were seen in the upper dermis of the melanotic macules, and no melanoma cells were observed in the upper dermis of the white macules which appeared at the site of regressed melanotic macules or nodules.In melanin‐laden cells observed in melanotic macules, many melanosome complexes with hydrolytic enzyme activity and a small number of single melanosomes were recognized. We assumed that some or many of the melanin‐laden, melanophage‐like cells in the upper dermis of a regressing malignant melanoma might be effete melanoma cells. Cell‐mediated immunity had been preserved while spontaneous regression of tumors was occurring.However, the patient died as a result of metastatic melanomas which involved the lymph nodes, liver, bones, brain, heart, kidney, lung and gastrointestinal tract.
TINEA VERSICOLOR AND EARWAX
Authors: Ono Tomomichi , Jono Masayoshi , Kuriya Norikazu 
Published: April 2015

ABSTRACTThe types of earwax which may manifest characteristics of the skin surface, were found to be related with the incidence of tinea versicolor caused by lipophilic fungus, Malassezia furfur. The condition of earwax is usually classified into 2 types, wet and dry. Of 223 Japanese cases of tinea versicolor examined in Kumamoto, wet earwax was found in 90 cases (40.9%). The results indicate a statistically significant higher incidence than in normal Japanese, among which the percentage of individuals with wet earwax is 16%.
SURVEY OF MATING TYPES OF CLINICAL ISOLATES OF MICROSPORUM CANIS IN JAPAN
Authors: Kinbara Takeshi , Fukushiro Ryoichi 
Published: April 2015

ABSTRACTThe results of mating experiments with isolates of Microsporum canis isolated in Japan were reported. Of the 120 cultures of M. canis studied, 111 originated from human cases and 9 from pet cats. Ninety‐eight of the isolates were compatible with Nannizzia otae and proved to be of the “—” mating type. Cleistothecia were not produced by 22 isolates in combination with the two compatible tester strains.
RETINOID STIMULATES EPIDERMAL OUTGROWTH OF PIG SKIN EXPLANTS
Authors: Aoyagi Takashi , Kamigaki Katsuko , Kato Naoko , Fukaya Toru , Iizuka Hajime , Miura Yusho 
Published: April 2015

ABSTRACTUsing a pig explant culture system, the effects of retinoids on pig epidermal cells were studied. Ro 10‐9359 slightly stimulated epidermal outgrowth, but this effect was not significant. Ro 10‐1670 (a metabolite of Ro 10‐9359) significantly stimulated epidermal outgrowth. Cytochalasin B and colchicine markedly inhibited the stimulatory effect of Ro 10‐1670, but hydroxyurea or cycloheximide did not completely block this stimulatory effect. During a migratory period, cytochalasin B completely blocked it. Neither Ro 10‐1670 nor Ro 10‐9359 effected a change in mitotic index. Histological studies revealed that Ro 10‐1670‐treated epidermal cells did not form any keratin layers. These results suggest that Ro 10‐1670 stimulated outgrowth, particularly migratory outgrowth, of epidermal cells, resulting in reduced keratin formation.
COAGULASE TYPES OF COAGULASE‐POSITIVE STAPHYLOCOCCI FROM BACTERIAL SKIN INFECTIONS
Authors: Dekio Satoshi , Onimura Kentaro 
Published: April 2015

ABSTRACTCoagulase types of the strains of coagulase‐positive Staphylococci isolated from bacterial skin infections were determined by the simplified method for coagulase typing. The abilities to utilize mannitol and to produce lipase and the sensitivities to antibiotics were also examined. Relationships were obvious between the coagulase types and the other characters as well as the studied dermatoses. Coagulase typing is considered to be a suitable method for the classification of coagulase‐positive Staphylococci in studies of staphylococcal skin infections, because the procedure is relatively simple, all strains of coagulase‐positive Staphylococci were classifiable in this study, and the results were useful for understanding staphylococcal skin infections.
CLINICAL AND HISTOLOGICAL STUDIES OF THE CLASSIFICATION AND THE NATURAL COURSE OF THE STRAWBERRY MARK
Authors: Nakayama Hiroko 
Published: April 2015

ABSTRACTApproximately 1250 cases of strawberry mark were encountered during a 16‐year period. The lesions were classified into three major types, (1) plaque type (d), (2) tumor type (Ds, DS, dS) and (3) subcutaneous type (S). The frequency of each clinical type (d, Ds, DS, dS, S) was studied for 1000 lesions. The plaque type was the first in frequency (57%) and the tumor type was the next (40%). The subcutaneous type was much less frequent (3%). The tendency toward spontaneous regression was studied for 308 lesions in 255 cases, which revealed differences in the natural course among clinical types. Lesions of the plaque and subcutaneous types regress with good end results. Those of the tumor type often regress incompletely. The prognosis of a lesion can be predicted fairly well once its clinical type is known. The clinical features of residual lesions have been briefly described. They are telangiectasia, skin redundancy with wrinkles, and protuberance. Histological investigation of 65 lesions in 62 cases including residual lesions showed hyaline degeneration in capillary walls. The pathological aspects of spontaneous regression have also been discussed.
GEOGRAPHICAL PATTERN OF PEMPHIGUS IN NORTH INDIA
Authors: Sehgal V.N. , Bharija S.C. , Sharma A.K. , Bikhchandani R. , Koranne R.V. 
Published: April 2015

ABSTRACTA clinical analysis of 70 patients suffering from various types of pemphigus is presented. It revealed a characteristic morphology, and occurrence in a relatively younger age group. Sex had hardly any impact on the disease. Pemphigus vulgaris constituted the largest group. Corticosteroids were the mainstay of its treatment although both lower initial and maintenance dosages were needed in our patients in contrast to those of the standard literature.
IMMUNOFLUORESCENCE STUDY OF DRUG‐INDUCED LICHEN PLANUS‐LIKE LESIONS
Authors: Watanabe Chiyeko , Hayashi Tsutomu , Kawada Akihiro 
Published: April 2015

ABSTRACTUsing an immunofluorescence technique, we investigated lesions of drug‐induced lichen planus‐like lesions from patients. In addition, a comparative study of idiopathic lichen planus from thirteen patients was made.Deposits of fibrinogen along the epidermal basement zone, IgM, and less often IgA, IgG, C1q and C3 on the colloid bodies were found in drug‐induced lichen planus‐like lesions. Similar deposits of immunoglobulins and complements were noted in 2 of 3 lesions developed by successful provocation tests. Deposits of fibrinogen were also demonstrated in all 3 lesions. However, no circulating antibodies directed toward the epidermal basement zone or other skin components were detected. No elevation of circulating immune complex levels was noted.Immunofluorescence findings in drug‐induced lichen planus‐like lesions were essentially identical to those in idiopathic lichen planus. This indicates that both conditions share a common disease process.
TREATMENT OF ERYTHEMATOUS DERMATOSES WITH POTASSIUM IODIDE
Authors: Horio Takeshi , Danno Kiichiro , Okamoto Hiroyuki , Miyachi Yoshiki , Furukawa Fukumi , Imamura Sadao 
Published: April 2015

ABSTRACTForty‐seven patients with various erythematous dermatoses were treated with systemic administration of potassium iodide. There were fifteen patients with erythema nodosum, ten with nodular vasculitis, fourteen with erythema multiforme, and eight with Sweet's syndrome. Potassium iodide was dramatically effective in a substantial number of cases. In responsive patients, improvement of the cutaneous changes was evident within a few days. The effect of the drug was impressive in patients with lesional tenderness, joint pains, and/or high fever. Relief of these subjective symptoms occurred within twenty‐four hours.The possible mode of the action of potassium iodide and pathogenesis of erythematous dermatoses were also discussed.
ANTIACTIVATOR IN PLASMA OF PATIENTS WITH BEHÇET'S DISEASE
Authors: Tsushima Hirofumi , Toki Naotika 
Published: April 2015

ABSTRACTIn the present study, it was confirmed for the first time that the plasminogen activator activity in the euglobulin fraction could be determined by the Testzym method using chromozyme S‐2251. Antiactivators in plasma of patients with Behçet's disease, who showed decreased blood fibrinolytic activity, could be precipitated together with the activator in the euglobulin fraction. It was confirmed that the antiactivator was inhibited by dextran sulfate.
POLYMORPHIC TYPE OF XANTHOMATOSIS IN ASSOCIATION WITH IgA MYELOMA
Authors: Hagiyama Masaharu , Arima Terukatsu , Arata Jiro , Namba Koji , Murakami Motoo , Inamizu Mariko , Nishimoto Masayoshi 
Published: April 2015

ABSTRACTClinical and immunochemical findings were reported from a 36‐year‐old woman with multiple xanthomas of various clinical types (plane xanthoma, tubero‐tendinous, papulo‐eruptive and follicular xanthomas), multiple myeloma (IgA, type L), and hyperlipemia with markedly elevated levels of plasma cholesterol and triglycerides. A bone marrow specimen showed foam cells and atypical plasma cell proliferation.Lipoprotein‐IgA complexes were thought to exist in the serum of this patient on gel filtration and micro‐ouchterlony test. Cutaneous xanthomas associated with multiple myeloma are plane xanthomas in most of the reported cases. Combination of several different types of xanthomas has been reported in only three cases, all of which were associated with IgA myeloma.
INDUSTRIAL CONTACT DERMATITIS DUE TO NITRO AND AMINO DERIVATIVES
Authors: Naniwa Shiro 
Published: April 2015

ABSTRACTUsing guinea pigs sensitized with 2‐amino‐4‐chlorophenol, cross sensitization between this compound and para‐aminophenol derivatives was studied. The results of the patch test showed no evidence of cross sensitization; however, the leucocyte adherence inhibition test suggested that cross sensitization may occur.
DISTRIBUTION OF GLYCOSAMINOGLYCANS IN DERMAL CONNECTIVE TISSUE FROM SCLERODERMA PATIENTS
Authors: Tajima Shingo , Nishikawa Takeji , Hatano Hitoshi , Nagai Yutaka 
Published: April 2015

ABSTRACTLesional skin specimens from scleroderma patients were sliced horizontally into three layers. The ratio of hyaluronic acid to dermatan sulfate in each layer was analyzed. The ratios in each layer of scleroderma skin were lower than those in the corresponding layers of normal skin and the lowest value was found in the bottom layer of scleroderma skin. The possible involvement of glycosaminoglycan metabolism in the pathophysiology of scleroderma is discussed.
SYRINGOMAS OF THE CLEAR CELL TYPE—AN ULTRASTRUCTURAL OBSERVATION—
Authors: Yamasaki Yuuichiro , Toda Michiko , Kitamura Keijiro 
Published: April 2015

ABSTRACTA case of a 72‐year‐old female with multiple syringomas of the clear cell type is reported. Ultrastructural observations revealed the following findings: 1) The tumor cells had the ordinary morphological features of usual syringoma cells. 2) The clear cells were rich in glycogen granules and various vacuoles. 3) Massive accumulations of glycogen granules surrounded by the unit membrane were occasionally observed. These electron microscopic findings were compatible with the light microscopic appearance of the clear cells. The tumor cells possessing characteristic clear cytoplasm can speculatively be regarded as altered syringoma cells with glycogen accumulation. The abnormal accumulation of the glycogen granules might be due to a kind of disorganized metabolism caused by diabetes mellitus in the syringoma cells.
Clq‐BINDING SUBSTANCES IN PEMPHIGUS
Authors: Miyagawa Sachiko , Morita Michiko , Sakamoto Kuniki 
Published: April 2015

ABSTRACTSerial serum samples from sixteen patients with pemphigus were assayed for Clq binding activity (ClqBA) using a modified solid phase Clq binding assay. The modification included use of horseradish peroxidase instead of 125I or 131I. ClqBA was detected in six of the sixteen patients. Sequential samples from those positive patients demonstrated a decrease in ClqBA in response to steroid therapy.
TYROSINASE AND γ‐GLUTAMYL TRANSPEPTIDASE IN 5‐S‐CYSTEINYLDOPA GENESIS WITHIN MELANOTIC AND AMELANOTIC MELANOMAS
Authors: Mojamdar Manoj , Ichihashi Masamitsu , Mishima Yutaka 
Published: April 2015

ABSTRACTThe roles of tyrosinase and γ‐glutamyl transpeptidase (γ‐GTP) in 5‐S‐cysteinyldopa (5‐S‐CD) genesis in melanotic and amelanotic melanoma cells has been investigated in vitro and in vivo. Complete inhibition of tyrosinase by 10–3 M diethyl dithiocarbamate has been found to partially inhibit dopa‐cysteine dependent 5‐S‐CD genesis by cultured melanoma cells. On the other hand iodoacetamide, an inhibitor of γ‐GTP, has been found to completely inhibit dopa‐glutathione dependent 5‐S‐CD genesis by these cells. Melanotic melanoma has been found to be rich in tyrosinase and γ‐GTP activity. In contrast, the amelanotic melanoma studied here was found to lack tyrosinase activity and to have very little γ‐GTP activity. The sub‐cellular distribution of these enzymes in melanotic melanoma cells has revealed that while the melanosome‐rich fraction is high in tyrosinase activity, it contains no substantial γ‐GTP activity. These findings indicate that 5‐S‐CD can form non‐enzymically in vitro from dopa and cysteine. Further, it also provides evidence that γ‐GTP is the enzyme that converts glutathione‐dopa to 5‐S‐CD within melanoma cells.
A COMPARATIVE STUDY ON ULTRASTRUCTURE AND IMMUNOLOGICAL PROPERTIES OF HUMAN PAPILLOMA VIRUS BEFORE AND
Authors: Fixation After , Kimura Shunji , Harada Reiko 
Published: April 2015

ABSTRACTIn order to clarify the effects of tissue processing on the ultrastructure and immunological properties of human papilloma virus (HPV), HPV particles were studied comparatively by negative staining and immunofluorescence (IF) technique before and after processing with various fixatives, such as formalin (F), ethanol (E), formalin and ethanol (FE), and formalin, ethanol, xylol and ethanol (FEXE). No significant ultrastructural alterations were demonstrated after processing with F, but considerable alterations were seen with E and FE. IF studies using antiserum against raw HPV revealed a remarkable decrease in specific fluorescence (SF) after processing with F or FEXE, and the disappearance of SF with E alone. However, IF studies using antiserum against formalin‐processed HPV revealed identical SF before and after processing with F or FEXE. From these findings, it is concluded that the ultrastructure of HPV may to some degree be altered by usual tissue processing, and that the reactivity of HPV to the antiserum is most seriously affected by formalin fixation of the tissue processing methods employed in preparing deparaffinized sections.
REITER'S DISEASE
Authors: Sehgal V.N. , Koranne R.V. , Basumatary R.K. , Vishwakarma G.K. , Singh A.K. , Raj Gita Ashok 
Published: April 2015

ABSTRACTA case of Reiter's disease in a twenty‐four year old male is described. He developed deformities of the big joints twelve years prior to the appearance of the skin eruptions. The clinical and radiological findings were fairly conclusive.
BARIUM GRANULOMA
Authors: Ishii Yoshimitsu , Inoue Shouhei , Kikuchi Ichiro , Taketomi Isao 
Published: April 2015

ABSTRACTA foreign body granuloma was produced subcutaneously after a golf ball exploded and a portion was injected into the skin. X‐ray diffractiometry and histological examination suggested that it was a barium granuloma.
EXPERIMENTAL DRUG‐INDUCED RASH AND SYSTEMIC CUTANEOUS BASOPHIL HYPERSENSITIVITY
Authors: Kitamura Kazuko , Ikezawa Zenro , Murakami Junko , Nagai Ryukichi 
Published: April 2015

AbstractGuinea pigs immunized with antibiotics (sodium cephalothin, CET) developed a systemic, delayed‐onset rash or so called generalized rash (GR) when challenged intraperitoneally with large amounts of CET seven weeks later.Skin test reactivity and GR intensity were most prominent at 24 hours. Microscopically, skin test reactions and flare‐up lesions of old skin test sites during GR were characterized by the onset of dermal infiltration of lymphocytes, striking dilatation and compaction of superficial venules at 24 hours, and papillary dermal infiltration of basophils at 48 hours.In addition, the animals that developed GR exhibited eosinophilia at 24 hours, which was followed by basophilia at 48 hours.GR developed with large doses of CET has properties similar to the so called SCBH designated by H.F. Dvorak.This experimental model may serve as a useful model for drug reactions in man which may involve SCBH.