bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fra-1 regulation of Matrix Metallopeptidase-1 (MMP-1) in metastatic variants of MDA-MB-231 breast cancer cells
Authors: Henckels Eric , Prywes Ron 
Published: October 2013

Matrix Metallopeptidase 1 (MMP-1) expression has repeatedly been correlated to tumorigenesis and metastasis. Yet, MMP-1 regulation in a metastatic context remains largely unknown. Here we confirm differential MMP-1 expression in mammary carcinoma cells with varied metastatic potentials. We show that MMP-1 expression is regulated by an AP-1 element in its promoter in highly metastatic MDA-MB-231 mammary carcinoma cell derivatives. Fra-1, an AP-1 family transcription factor, differentially binds this element in highly metastatic cells compared to low metastatic cells and is required for MMP-1 expression. Overexpression of Fra-1 also caused increased MMP-1 expression. Fra-1 mRNA levels are unchanged in the cell variants, however its protein levels are higher in the metastatic cells. While there was no change in Fra-1 protein degradation rates, protein synthesis of Fra-1 was increased in the metastatic cell variant. These results demonstrate that Fra-1 and MMP-1 levels are differentially regulated in metastatic cell variants at the level of Fra-1 protein translation. Consistent with the importance of Fra-1 for tumor growth, we found that Fra-1 overexpression was sufficient to increase cell motility and anchorage independent growth. These results suggest that increased Fra-1 translation is critical for regulation of MMP-1 and tumor cell metastasis.
Association of Hospital and Provider Types on Sickle Cell Disease Outcomes
Authors: Jan Sophia , Slap Gail , Smith-Whitley Kim , Dai Dingwei , Keren Ron , Rubin David M. 
Published: October 2013

OBJECTIVES:Adolescents and young adults (A/YA) with sickle cell disease (SCD) are hospitalized in both children’s and general hospitals. We determined the effect of hospital type and provider specialty on outcomes of hospitalized A/YA with SCD and acute chest syndrome (ACS).METHODS:This retrospective cohort study used the 2007–2009 Premier Database, a large multi-institutional database, to identify 1476 patients ages 16 to 25 years with 2299 admissions with SCD and ACS discharged from 256 US hospitals from 2007 to 2009. Multilevel logistic regression and zero-truncated negative binomial regression were performed after adjustment for patient demographic, clinical, and hospital characteristics to test the association of hospital type and provider specialty on death, endotracheal intubation, simple or exchange transfusion, length of stay (LOS), and 30-day readmission.RESULTS:Of all admissions, 14 died and 45% were intubated. General hospitals had 13 deaths and were associated with higher intubation rates (predicted probability [PP], 48% [95% confidence interval (CI), 43%–52%]) and longer LOS (predicted mean LOS, 7.6 days [95% CI, 7.2–7.9]) compared with children’s hospitals (PP of intubation, 24% [95% CI, 5%–42%]; and predicted mean LOS, 6.8 days [95% CI, 5.6–5.8]). There was no difference by hospital type or provider specialty in PP of simple or exchange transfusion, or 30-day readmission.CONCLUSIONS:General hospitals carry higher intubation risks for A/YA with SCD and ACS compared with children’s hospitals. We need to better understand the drivers of these differences, including the role of staff expertise, hospital volume, and quality of ongoing SCD care.
Influenza-Associated Pediatric Deaths in the United States, 2004–2012
Authors: Wong Karen K. , Jain Seema , Blanton Lenee , Dhara Rosaline , Brammer Lynnette , Fry Alicia M. , Finelli Lyn 
Published: October 2013

BACKGROUND:
Influenza-associated deaths in children occur annually. We describe the epidemiology of influenza-associated pediatric deaths from the 2004–2005 through the 2011–2012 influenza seasons.

METHODS:
Deaths in children &lt;18 years of age with laboratory-confirmed influenza virus infection were reported to the Centers for Disease Control and Prevention by using a standard case report form to collect data on demographic characteristics, medical conditions, clinical course, and laboratory results. Characteristics of children with no high-risk medical conditions were compared with those of children with high-risk medical conditions.

RESULTS:
From October 2004 through September 2012, 830 pediatric influenza–associated deaths were reported. The median age was 7 years (interquartile range: 1–12 years). Thirty-five percent of children died before hospital admission. Of 794 children with a known medical history, 43% had no high-risk medical conditions, 33% had neurologic disorders, and 12% had genetic or chromosomal disorders. Children without high-risk medical conditions were more likely to die before hospital admission (relative risk: 1.9; 95% confidence interval: 1.6–2.4) and within 3 days of symptom onset (relative risk: 1.6; 95% confidence interval: 1.3–2.0) than those with high-risk medical conditions.

CONCLUSIONS:
Influenza can be fatal in children with and without high-risk medical conditions. These findings highlight the importance of recommendations that all children should receive annual influenza vaccination to prevent influenza, and children who are hospitalized, who have severe illness, or who are at high risk of complications (age &lt;2 years or with medical conditions) should receive antiviral treatment as early as possible.
Bridging Organizational Divides in Health Care: An Ecological View of Health Information Exchange
Authors: Unertl Kim M , Johnson Kevin B , Gadd Cynthia S , Lorenzi Nancy M 
Published: October 2013

Background
The fragmented nature of health care delivery in the United States leads to fragmented health information and impedes patient care continuity and safety. Technologies to support interorganizational health information exchange (HIE) are becoming more available. Understanding how HIE technology changes health care delivery and affects people and organizations is crucial to long-term successful implementation.

Objective
Our study investigated the impacts of HIE technology on organizations, health care providers, and patients through a new, context-aware perspective, the Regional Health Information Ecology.

Methods
We conducted more than 180 hours of direct observation, informal interviews during observation, and 9 formal semi-structured interviews. Data collection focused on workflow and information flow among health care team members and patients and on health care provider use of HIE technology.

Results
We structured the data analysis around five primary information ecology components: system, locality, diversity, keystone species, and coevolution. Our study identified three main roles, or keystone species, involved in HIE: information consumers, information exchange facilitators, and information repositories. The HIE technology impacted patient care by allowing providers direct access to health information, reducing time to obtain health information, and increasing provider awareness of patient interactions with the health care system. Developing the infrastructure needed to support HIE technology also improved connections among information technology support groups at different health care organizations. Despite the potential of this type of technology to improve continuity of patient care, HIE technology adoption by health care providers was limited.

Conclusions
To successfully build a HIE network, organizations had to shift perspectives from an ownership view of health data to a continuity of care perspective. To successfully integrate external health information into clinical work practices, health care providers had to move toward understanding potential contributions of external health information. Our study provides a foundation for future context-aware development and implementation of HIE technology. Integrating concepts from the Regional Health Information Ecology into design and implementation may lead to wider diffusion and adoption of HIE technology into clinical work.
Experiences with Autonomy: Learners’ Voices on Language Learning
Authors: Kristmanson Paula , Lafargue Chantal , Culligan Karla 
Published: October 2013

Abstract: This article focuses on the experiences of Grade 12 students using a language portfolio based on the principles and guidelines of the European Language Portfolio (ELP) in their second language classes in a large urban high school. As part of a larger action-research project, focus group interviews were conducted to gather data related to experiences and perceptions regarding second language learning. In particular, the research questions focused on the learners’ experiences with the language portfolio and its potential role in contributing to learner autonomy. Three overarching themes emerged from the data collected from learners who participated in this study. These themes related to general perceptions of learning, to language learning experiences, and to experiences with the language portfolio. The article concludes with a discussion of the pedagogical linkages to learner autonomy and suggests implications for educators who wish to promote learner autonomy.
le vieillissement des populations et des territoires au prisme d'une ruralité transformée
Authors: Gucher Catherine 
Published: October 2013

Sous l’effet de l’urbanisation croissante de notre pays, ce qu’il était convenu jusqu’alors de nommer le milieu rural, se transforme : villes et campagnes s’interpénètrent davantage et la mobilité des populations s’accroît. Les espaces ruraux présentent désormais des visages divers, tant pour ce qui est de leurs caractéristiques physiques que pour leur morphologie sociale et culturelle. Cependant ces territoires demeurent plus que les zones urbaines concernés par le vieillissement. Il importe alors de comprendre comment les populations qui les peuplent organisent leurs parcours de vie au fil du vieillissement et dans la vieillesse et comment les instances territoriales prennent en compte ces phénomènes de vieillissement, à travers des politiques d’aménagement et de développement de services aux populations.
Des transitions africaines au monde arabe, 1991-2011 : vent de printemps sur les outils de la transitologie
Authors: Thiriot Céline 
Published: October 2013

La comparaison entre les processus de transition en Afrique subsaharienne dans les années 1990 et les mouvements du « printemps arabe » depuis 2011, permet de dresser un bilan critique mais nuancé de la « transitologie ». Les enseignements des transitions africaines conduisent à revenir sur les outils de la « transitologie », et spécifiquement le découpage séquentiel et la place et le rôle dévolus aux élections. Il s’agit d’éclairer autrement la lecture proposée du « Printemps arabe ».
Age of riverine carbon suggests rapid export of terrestrial primary production in tropics
Authors: Martin Erin E. , Ingalls Anitra E. , Richey Jeffrey E. , Keil Richard G. , Santos Guaciara M. , Truxal Laura T. , Alin Simone R. , Druffel Ellen R. M. 
Published: October 2013

AbstractThe balance between the storage of vascular plant carbon in soils, oxidation to carbon dioxide, and export via rivers affects calculations of the strength of terrestrial ecosystems as carbon sinks. The magnitude and timescale of the riverine export pathway are not well constrained. Here we use radiocarbon dating of lignin phenols to show that plant‐derived carbon carried by suspended sediment of the Mekong River is very young, having been produced within the last 18 years. Further, this plant‐derived carbon remains young during times of the year when bulk carbon varies from modern to over 3000 radiocarbon years old. Our results demonstrate that primary‐production derivatives are exported rapidly and suggest that the age of riverine lignin is similar to estimates of the residence time of terrestrial organic carbon in tropical catchments. These results are relevant for modeling predictions of the influence of the terrestrial biosphere on atmospheric carbon dioxide levels.
Continued decline of South Pole neutron monitor counting rate
Authors: Bieber John , Clem John , Evenson Paul , Oh Suyeon , Pyle Roger 
Published: October 2013

AbstractThe counting rate of a neutron monitor at South Pole, Antarctica, displays a long‐term decline over the 49 year span from March 1964 to the present. The counting rate follows an 11 year cycle with maxima at times of low solar activity. However, after adjusting for the unusually high overall cosmic ray fluxes in 2009, we find that the 2009 peak rate (based on 27 day averages) was approximately 10% lower than the 1965 peak rate. This change is much larger than that recorded by any other neutron monitor. We suggest that the South Pole monitor, owing to its unique position at both high latitude and high altitude may have a hitherto unsuspected sensitivity to secular changes in the magnetic field of the Earth.
Are ionospheric storms the same during different solar cycles?
Authors: Mendillo Michael , Narvaez Clara , Marusiak Angela G. 
Published: October 2013

AbstractThe ionosphere's response to geomagnetic storms has been studied since the earliest days of terrestrial space physics. In terms of temporal coverage, the largest data sets used extensively have been from the global network of ionosondes. Many previous investigations examined the behavior of the F layer's maximum electron density (Nmax)—often contrasting the difference seen between storms that occur during solar maximum years versus those during solar minimum years. We report on the first attempt to study systematically the patterns of ionospheric disturbance seen during different solar cycles. We select two midlatitude sites with long‐term consistency in data: Wallops Island (Virginia) and Hobart (Tasmania)—stations with comparable geographic and geomagnetic coordinates—but in different hemispheres and widely separated longitude sectors. We compare average ionospheric storm patterns using over 200 moderate to severe geomagnetic storms within each of solar cycle #20 (October 1964 to June 1976) and cycle #23 (May 1996 to December 2008). We compute average patterns of ΔNmax(%), measured with respect to monthly mean conditions, following storm and local time. The overall results show remarkable consistency in characteristic patterns of an ionospheric storm: a short positive phase that occurs during the daytime hours on the first day of a storm, with a prolonged negative phase on subsequent days. Statistical differences occur in the overall magnitudes and longevities of these patterns, consistently showing that cycle #23 had less severe ionospheric storms. An analysis of geomagnetic indices shows that degrees of disturbance were, in fact, lower during solar cycle #23 than cycle #20.
Activation of IL‐27 signalling promotes development of postinfluenza pneumococcal pneumonia
Authors: Cao Ju , Wang Dongsheng , Xu Fang , Gong Yi , Wang Hong , Song Zixin , Li Dageng , Zhang Hua , Li Dairong , Zhang Liping , Xia Yun , Xu Huajian , Lai Xaiofei , Lin Shihui , Zhang Xuemei , Ren Guosheng , Dai Yubing , Yin Yibing 
Published: October 2013

AbstractPostinfluenza pneumococcal pneumonia is a common cause of death in humans. However, the role of IL‐27 in the pathogenesis of secondary pneumococcal pneumonia after influenza is unknown. We now report that influenza infection induced pulmonary IL‐27 production in a type I IFN‐α/β receptor (IFNAR) signalling‐dependent manner, which sensitized mice to secondary pneumococcal infection downstream of IFNAR pathway. Mice deficient in IL‐27 receptor were resistant to secondary pneumococcal infection and generated more IL‐17A‐producing γδ T cells but not αβ T cells, thereby leading to enhanced neutrophil response during the early phase of host defence. IL‐27 treatment could suppress the development of IL‐17A‐producing γδ T cells activated by Streptococcus pneumoniae and dendritic cells. This suppressive activity of IL‐27 on γδ T cells was dependent on transcription factor STAT1. Finally, neutralization of IL‐27 or administration of IL‐17A restored the role of γδ T cells in combating secondary pneumococcal infection. Our study defines what we believe to be a novel role of IL‐27 in impairing host innate immunity against pneumococcal infection.
Use of prototyping in preoperative planning for patients with head and neck tumors
Authors: de Farias Terence Pires , Dias Fernando Luiz , Galvão Mário Sérgio , Boasquevisque Edson , Pastl Ana Carolina , Albuquerque Sousa Bruno 
Published: October 2013

AbstractBackgroundPrototyping technologies for reconstructions consist of obtaining a 3‐dimensional model of the object of interest. Solid models are constructed by the deposition of materials in successive layers. The purpose of this study was to perform a double‐blind, randomized, prospective study to evaluate the efficacy of prototype use in head and neck surgeries.MethodsThirty‐seven cases were randomized into prototype and nonprototype groups. The following factors were recorded: the time of plate and locking screw apposition, flap size, time for reconstruction, and an aesthetic evaluation.ResultsThe prototype group exhibited a reduced surgical time (43.7 minutes vs 127.7 minutes, respectively; p = .001), a tendency to reduce the size of the bone flap taken for reconstruction, and better aesthetic results than the group that was not prototyped.ConclusionThe use of prototyping demonstrated a trend toward a reduced surgical time, smaller bone flaps, and better aesthetic results. © 2014 Wiley Periodicals, Inc. Head Neck 36: 1773–1782, 2014
Enhanced function of TRPV1 via up‐regulation by insulin‐like growth factor‐1 in a rat model of bone cancer pain
Authors: Li Y. , Cai J. , Han Y. , Xiao X. , Meng X.L. , Su L. , Liu F.Y. , Xing G.G. , Wan Y. 
Published: October 2013

AbstractBackgroundUp‐regulation of transient receptor potential vanilloid subfamily, member 1 (TRPV1) is associated with the development and maintenance of cancer pain. The present study aimed to investigate the electrophysiological function of the up‐regulated TRPV1 and the potential regulatory effects of insulin‐like growth factor‐1 (IGF‐1) on TRPV1 expression in peripheral nerves in a rat model of bone cancer pain.MethodsA bone cancer pain model of rats was established by injecting MRMT‐1 (rat mammary gland carcinoma cells) breast cancer cells into the tibia bone cavity. Thermal hyperalgesia was assessed by paw‐withdrawal latency to a thermal stimulus, and mechanical allodynia was measured with von Frey monofilaments. TRPV1 and IGF‐1 expression were examined with immunohistochemical staining and Western blot. TRPV1 current density of dorsal root ganglion (DRG) neurons was measured with whole‐cell patch clamping recording technique.ResultsRats showed thermal hyperalgesia and mechanical allodynia 14–21 days after MRMT‐1 inoculation into the tibia bone marrow. TRPV1 protein expression and its current density increased in DRG neurons. At the same time, IGF‐1 expression increased in tibia bone cavity, and IGF‐1 incubation increased total or membrane TRPV1 protein expression and TRPV1 current in primary cultured DRG neurons. Inhibition of IGF‐1 receptors in vivo reversed mechanical allodynia and thermal hyperalgesia in rats with bone cancer pain.ConclusionOur results provide novel evidence for the increase of IGF‐1 in tibia bone marrow, which is responsible for the up‐regulation of TRPV1 expression and function in the peripheral nerves of bone cancer pain rats.
Preparation of cardiac extracellular matrix scaffolds by decellularization of human myocardium
Authors: Oberwallner Barbara , Brodarac Andreja , Choi Yeong‐Hoon , Saric Tomo , Anić Petra , Morawietz Lars , Stamm Christof 
Published: October 2013

AbstractExtracellular matrix (ECM) derived by tissue decellularization has applications as a tissue engineering scaffold and for support of cellular regeneration. Myocardial ECM from animals has been produced by whole‐organ perfusion or immersion processes, but methods for preparation of human myocardial ECM for therapy and research have not been compared in detail, yet. We analyzed the impact of decellularization processes on human myocardial ECM, and tested its ability to serve as a scaffold for cell seeding. Sodium dodecyl sulfate (SDS)‐based decellularization, but not treatments based on Triton X‐100, deoxycholate or hypo/hypertonic incubations, removed cells satisfactorily, and incubation with fetal bovine serum (FBS) eliminated residual DNA. ECM architecture was best preserved by a protocol consisting of 2 h lysis, 6 h SDS, and 3 h FBS, but age and pathology of the donor tissue are highly important for producing reproducible, high‐quality scaffolds. We also studied ECM repopulation with mesenchymal stem cells (CB‐MSC), cardiomyocytes derived from induced pluripotent stem cells (iPS‐CM), and naïve neonatal mouse cardiomyocytes. Cells attached to the matrix and proliferated and displayed higher viability than in standard culture. We conclude that human cardiac ECM sheets may be suitable scaffold for cell‐matrix interaction studies and as a biomaterial for tissue regeneration and engineering. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3263–3272, 2014
A Novel Visible Spectrophotometric Method for the Determination of Methanol Using Sodium Nitroprusside as Spectroscopic Probe
Authors: Zhan Yan‐Yan , Zhang Yan , Li Quan‐Min , Du Xin‐Zhen 
Published: October 2013

AbstractA novel, rapid and reliable method has been established for the determination of methanol using sodium nitroprusside as a spectroscopic probe. This method indicates that the sodium nitroprusside can react with the methanol to form a colored product in the basic solution. The absorbance of the product is measured at the maximal absorption wavelength of 481 nm, and the amount of methanol can be calculated based on this absorbance. A good linear relationship of the concentration of methanol versus absorbance is observed with a linear range of 0.02‐6.0 mg mL−1. The linear regression equation is A = 0.02484 + 0.29457C (mg mL−1), with a correlation coefficient of 0.9991. The detection limit (3σ/k) is 0.012 mg mL−1, while its R.S.D. is 1.5% and the recovery rate is 97.5‐102.5%. The parameters with regard to determination are optimized, and the reaction mechanism is discussed. This method has been successfully applied to determine methanol in variety of samples. Analytical results obtained by this new method were very gratifying.
MRI hemodynamic markers of progressive bicuspid aortic valve‐related aortic disease
Authors: Hope Michael D. , Sigovan Monica , Wrenn S. Jarrett , Saloner David , Dyverfeldt Petter 
Published: October 2013

PurposeTo determine the reproducibility of MRI aortic hemodynamic markers and to assess their relationship to aortic growth in a cohort of patients with bicuspid aortic valves (BAV).Materials and MethodsTwenty‐five patients previously studied with four‐dimensional (4D) Flow imaging who had at least two separate cross‐sectional imaging studies to assess for aortic growth were included: tricuspid aortic valve (TAV) controls without valvular disease (n = 12) and patients with BAV (n = 13). Flow data from the ascending aorta was used for calculation of peak velocity, normalized flow displacement, maximum wall shear stress (WSS), mean WSS, and minimal WSS. Pearson's correlation was used to evaluate interobserver agreement, and linear regression to evaluate the correlation between the different hemodynamic markers and growth. Patient informed consent was waived by the institutional review board that approved the study.ResultsPeak velocity and flow displacement were very reproducible (r = 0.90–1.0 and r = 0.91–0.98, respectively). The range of WSS parameters was largely reproducible (0.47 < r < 0.96) with the greatest variability at the data extraction stage of analysis (0.47 < r < 0.85). Flow displacement best correlated with interval aortic growth (r = 0.65), peak velocity was moderately correlated (r = 0.35), but the WSS parameters did not correlate well with growth (r < 0.17).ConclusionFlow displacement is a simple and reproducible hemodynamic marker that shows good correlation with aortic growth in patients with bicuspid aortic valves. J. Magn. Reson. Imaging 2014;40:140–145. © 2013 Wiley Periodicals, Inc.