bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Key Factor for Ultrafast Rates of Photoinduced Electron Transfer among Five Flavin Mononucleotide Binding Proteins: Effect of Negative, Positive, and Neutral Charges at Residue 13 on the Rate
Authors: Taniguchi Seiji , Chosrowjan Haik , Tanaka Fumio , Nakanishi Takeshi , Sato Shuta , Haruyama Yoshihiro , Kitamura Masaya 
Published: March 2013

Abstract
Crystal structures of E13K [Glu13 with negative charge in wild type (WT) was replaced by Lys with positive charge] and E13R (Glu13 was replaced by Arg with positive charge) of flavin mononucleotide-binding protein (FMN-bp) from Desulfovibrio vulgaris (Miyazaki F) were determined by X-ray diffraction method. Ultrafast fluorescence dynamics of FMN in these proteins were measured by fluorescence up-conversion. The average lifetimes of E13K and E13R were 0.198 and 0.186 ps, which are compared to the reported lifetimes of WT, E13T (Glu13 was replaced by Thr with neutral charge) and E13Q (Glu13 was replaced by Gln with neutral charge) FMN-bp, 0.220, 0.872, and 1.10 ps, respectively. These ultrashort lifetimes are ascribed to photoinduced electron transfer (ET) from Trp32, Tyr35, and Trp106 to the excited isoalloxazine. The observed lifetimes of the five FMN-bp isoforms were simultaneously analyzed with both Marcus and Hush, and Kakitani and Mataga ET theories, in order to characterize the ET mechanisms. It was concluded that the electrostatic (ES) energy between the photoproducts and ionic charges inside the proteins is a key factor for the ET rate in the flavoproteins.
Hybrid Breeding in Barley
Authors: Mühleisen Jonathan , Maurer Hans Peter , Stiewe Gunther , Bury Paul , Reif Jochen C. 
Published: March 2013

ABSTRACTBarley (Hordeum vulgare L.) has been largely neglected in hybrid breeding research of autogamous cereals. The objectives of our study were (i) to study the amount of heterosis, (ii) examine the potential to predict hybrid performance based on midparent values or general combining ability (GCA) effects, and (iii) draw conclusions on the prospects for hybrid breeding in barley. Grain yield performance of 124 hybrids of six‐rowed winter barley, their 27 male and 38 female parental lines, as well as nine line and six hybrid varieties and one hybrid in registration was investigated in plot‐based multi‐environment trials. In a two‐stage analysis, best linear unbiased estimators and variance components of hybrids and parental lines were calculated. Midparent heterosis averaged 11.3%, with a range from 0.7 to 19.9%. Better‐parent heterosis was slightly lower with an average of 9.2%. Maximum commercial heterosis (i.e., the difference between the hybrid performance and the performance of the best line variety) was 7.6%, which clearly underlines the significance of hybrid barley breeding. Accuracy to predict hybrid performance was only moderate based on midparent values (r = 0.46; P < 0.001) and GCA effects (r = 0.38; P < 0.001). Consequently, there is a need for alternative approaches to predict hybrid performance in barley.
Robust design of power system stabilizer using bacterial foraging algorithm
Authors: Abdul Hameed K. , Palani S. 
Published: March 2013

Abstract
In this paper, a novel bacterial foraging algorithm (BFA) based approach for robust and optimal design of PID controller connected to power system stabilizer (PSS) is proposed for damping low frequency power oscillations of a single machine infinite bus bar (SMIB) power system. This paper attempts to optimize three parameters (Kp, Ki, Kd) of PID-PSS based on foraging behaviour of Escherichia coli bacteria in human intestine. The problem of robustly selecting the parameters of the power system stabilizer is converted to an optimization problem which is solved by a bacterial foraging algorithm with a carefully selected objective function. The eigenvalue analysis and the simulation results obtained for internal and external disturbances for a wide range of operating conditions show the effectiveness and robustness of the proposed BFAPSS. Further, the time domain simulation results when compared with those obtained using conventional PSS and Genetic Algorithm (GA) based PSS show the superiority of the proposed design.
Use of microscope-integrated near-infrared indocyanine green videoangiography in the surgical treatment of intramedullary cavernous malformations: report of 8 cases
Authors: Endo Toshiki , Aizawa-Kohama Misaki , Nagamatsu Kenichi , Murakami Kensuke , Takahashi Akira , Tominaga Teiji 
Published: March 2013

Object
The characteristics and efficacy of indocyanine green (ICG) videoangiography in cavernous malformation (CM) have not been fully elucidated. The purpose of this paper is to examine the potential utility of ICG videoangiography in the surgical treatment of intramedullary CMs.

Methods
The authors conducted a retrospective review of 8 cases involving 5 men and 3 women who had undergone surgery for intramedullary CM between January 2008 and July 2011. All patients were evaluated by means of MRI. The MRI findings and clinical history in all cases suggested intramedullary CM as a preoperative diagnosis. In 2 of 8 cases, dilated venous structures associated with CMs were demonstrated. In one of these cases, there were coexisting extramedullary CMs. Intraoperatively, ICG fluorescence was observed for 5 minutes using microscope-integrated videoangiography.

Results
In all 8 cases, intra- and extramedullary CMs were seen as avascular areas on ICG videoangiography. Indocyanine green videoangiography helped surgeons to localize and predict margins of the lesions before performing myelotomy. Importantly, in the cases with associated venous anomalies, ICG videoangiography was useful in delineating and preserving the venous structures. In extramedullary CMs located dorsal to the spinal cord, gradual ICG infiltration was seen, starting at 110 seconds and maximal at 210 seconds after injection. Postoperative MRI confirmed total removal of the lesions in all cases, and subsequent recovery of all patients was uneventful.

Conclusions
Indocyanine green videoangiography provided useful information with regard to the detection of lesion margins by demonstrating intramedullary CMs as avascular areas. In cases associated with venous anomalies, ICG contributed to safe and complete removal of the CMs by visualizing the venous structure. In extramedullary CMs, ICG videoangiography demonstrated the characteristic of slow blood flow within CMs.
Inhibition of epidural fibrosis after microendoscopic discectomy with topical application of mitomycin C: a randomized, controlled, double-blind trial
Authors: Liu Lei , Sui Tao , Hong Xin , Wu Xiaotao , Cao Xiaojian 
Published: March 2013

Object
The authors conducted a study to evaluate the effects and the safety of locally applied mitomycin C (MMC) on epidural fibrosis after microendoscopic discectomy (MED).

Methods
Seventy-five patients undergoing single-level unilateral MED for lumbar disc herniation were randomly assigned to receive cotton wool impregnated with either 0.5 mg/ml MMC or saline applied at the site of discectomy for 5 minutes. Outcome measures included degrees of pain severity, functional disability, physical symptoms, and quantitative evaluation of postoperative epidural fibrosis shown on follow-up lumbar contrast-enhanced MRI.

Results
Sixty-two patients completed the follow-up. Neither serious drug adverse effects nor clinically significant laboratory adverse effects were observed. Patients in both groups showed similar clinical recoveries postoperatively. A statistically significant difference (p < 0.05) between the 2 treatments was shown in a quantitative evaluation of postoperative MRI-documented epidural fibrosis in the MMC group and the saline group using a modified grading system. The mean cross-sectional areas of epidural fibrosis were 7.32–70.06 mm2 in the MMC group and 22.94–90.48 mm2 in the saline group. The epidural fibrosis index ranged from 0.0296 to 0.3267 in the MMC group and from 0.1191 to 0.3483 in the saline group. A significant difference was also observed using the Ross grading system to evaluate postoperative MR images.

Conclusions
Although no benefit was observed clinically, the authors observed a notable reduction of epidural fibrosis after MED radiologically, with 0.5 mg/ml MMC locally applied and no clinical side effects. Clinical trial registration no.: ChiCTR-TRC-10001079 (http://www.chictr.org/cn/proj/show.aspx?proj=326).
Catheter fixation and ligation: a simple technique for ventriculostomy management following endovascular stenting
Authors: Sweeney Justin M. , Vasan Rohit , van Loveren Harry R. , Youssef A. Samy , Agazzi Siviero 
Published: March 2013

The object of this study was to describe a unique method of managing ventriculostomy catheters in patients on antithrombotic therapy following endovascular treatment of ruptured intracranial aneurysms. The authors retrospectively reviewed 3 cases in which a unique method of ventriculostomy management was used to successfully avoid catheter-related hemorrhage while the patient was on dual antiplatelet therapy. In this setting, ventriculostomy catheters are left in place and fixed to the calvarium with titanium straps effectively ligating them. The catheter is divided and the distal end is removed. The proximal end can be directly connected to a distal shunt system during this stage or at a later date if necessary. The method described in this report provided a variety of management options for patients requiring external ventricular drainage for subarachnoid hemorrhage. No patient suffered catheter-related hemorrhage.
This preliminary report demonstrates a safe and effective method for discontinuing external ventricular drainage and/or placing a ventriculoperitoneal shunt in the setting of active coagulopathy or antithrombotic therapy. The technique avoids both the risk of hemorrhage related to catheter removal and reinsertion and the thromboembolic risks associated with the reversal of antithrombotic therapy. Some aneurysm centers have avoided the use of stent-assisted coiling in cases of ruptured aneurysms to circumvent ventriculostomy-related complications; however, the method described herein should allow continued use of this important treatment option in ruptured aneurysm cases. Further investigation in a larger cohort with long-term follow-up is necessary to define the associated risks of infection using this method.
Imaging detection of endolymphatic sac tumor–associated hydrops
Authors: Butman John A. , Nduom Edjah , Kim H. Jeffrey , Lonser Russell R. 
Published: March 2013

Object
To determine if physiologically based MRI sequences can be used to detect endolymphatic sac tumor (ELST)–associated hydrops, the authors performed contrast-enhanced delayed FLAIR imaging in consecutive ELST patients with clinical findings consistent with hydrops.

Methods
Consecutive patients with von Hippel-Lindau (VHL) disease and clinical findings of endolymphatic hydrops and ELSTs underwent contrast-enhanced delayed FLAIR MRI. Clinical, audiological, operative, and imaging findings were analyzed.

Results
Three patients (2 male, 1 female) with 4 ELSTs (1 patient had bilateral ELSTs) were identified who had clinical findings consistent with endolymphatic hydrops. Computed tomography and MRI evidence of an ELST was found in all patients. Their mean age at initial evaluation was 39.7 years (range 28–51 years). All patients demonstrated progressive sensorineural hearing loss that was associated with episodic vertigo and tinnitus. Contrast-enhanced delayed FLAIR MRI clearly demonstrated dilation of the membranous labyrinth consistent with hydrops in the affected ears but not the unaffected ears. Two patients underwent resection of the associated ELST that resulted in stabilization of progressive hearing loss, as well as amelioration of tinnitus and vertigo.

Conclusions
Contrast-enhanced delayed FLAIR MRI can be used to detect ELST-associated hydrops. Noninvasive MRI detection of hydrops can permit earlier detection of ELSTs in patients with VHL disease and provides direct insight into a mechanism that underlies ELST-associated audiovestibular morbidity.
A Fast High Order Iterative Solver for the Electromagnetic Scattering by Open Cavities Filled with the Inhomogeneous Media
Authors: Zhao Meiling 
Published: March 2013

AbstractThe scattering of the open cavity filled with the inhomogeneous media is studied. The problem is discretized with a fourth order finite difference scheme and the immersed interface method, resulting in a linear system of equations with the high order accurate solutions in the whole computational domain. To solve the system of equations, we design an efficient iterative solver, which is based on the fast Fourier transformation, and provides an ideal preconditioner for Krylov subspace method. Numerical experiments demonstrate the capability of the proposed fast high order iterative solver.
Revision Hip Replacement in Patients 55 Years of Age and Younger
Authors: Konan Sujith , Soler Agustine , Haddad Fares S. 
Published: March 2013

The purpose of this study was to assess the efficacy and complications associated with revision hip arthroplasty undertaken in patients under 55 years of age. A review of our revision hip database showed that 430 revision hip arthroplasties undertaken over the past 9 years had a minimum follow-up of 2 years. Of these, 84 (46 female, 38 male) patients were under 55 years of age. The index procedures were preformed at an average age of 46 years (range 15 years to 54 years). The etiology of failure was aseptic loosening (74%), followed by infection (8%) and recurrent dislocation (7%). In the majority of cases, uncemented implants were used at revision surgery (91% uncemented femoral stem, 100% uncemented acetabular cup). A form of bone graft was necessary in 39% of cases. At final follow-up satisfactory progress was noted in all cases with a statistically significant improvement in Harris hip score (p<0.05). Revision of hip arthroplasty in patients below 55 years of age is associated with significant improvement in function and patient satisfaction.
Effectiveness of Platelet-rich Plasma Injection for Rotator Cuff Tendinopathy: A Prospective Open-label Study
Authors: Scarpone Michael , Rabago David , Snell Edward , Demeo Patrick , Ruppert Kristine , Pritchard Perry , Arbogast Gennie , Wilson John J. , Balzano John F. 
Published: March 2013

Objective: Assess platelet rich plasma (PRP) injection for rotator cuff tendinopathy (RCT). Design: Prospective open label study with 1-year follow-up. Methods: Participants recruited from an outpatient sports medicine clinic had clinically and magnetic resonance image (MRI)—demonstrated RCT refractory to physical therapy and corticosteroid injection. They received one ultrasound-guided injection of 3.0 mL of 1% xylocaine followed by 3.5 mL of PRP at the lesion and surrounding tendon. Primary outcome: 0—10 visual analog scale (VAS; baseline, 8, 12, and 52 weeks). Secondary outcomes: functional shoulder tests assessing rotator cuff strength and endurance (at baseline and 8 and 12 weeks), MRI severity (1—5 points [at baseline and 4 and 8 weeks]), and patient satisfaction (52 weeks). Results: Eighteen participants with 19 assessed shoulders reported VAS pain score improvement from 7.5 ± 0.3 points to 0.5 ± 0.3 points by week 12 and 0.4 ± 0.2 (P = .0001) points at week 52. Functional outcomes significantly improved; the largest effect was seen in the external rotation test: 33.5 ± 5.7 seconds to 62.6 ± 7.2 seconds at week 12 (P = .0001). MRI appearance improved by 1 to 3 points in 16 of 18 assessed shoulders. Seventeen participants were “completely satisfied” (12) or “satisfied” (5). One participant was “unsatisfied.” Conclusions: A single ultrasound-guided, intralesional injection of PRP resulted in safe, significant, sustained improvement of pain, function, and MRI outcomes in participants with refractory RCT. Randomized multidisciplinary effectiveness trials that add ultrasound and validated clinical outcome measures are needed to further assess PRP for RCT.
Study of Gel Growth Cobalt (II) Oxalate Crystals as Precursor of Co3O4 Nano Particles
Authors: Prananto Yuniar Ponco , Khunur Mohammad Misbah , Wahyuni Dini Tri , Shobirin Rizky Arief , Nata Yoga Rizky , Riskah Efiria 
Published: March 2013

Crystal growth of cobalt (II) oxalate in silica gel at room temperature as precursor of Co3O4 nano particles has been studied. Specifically, this project is focusing on the use of two different reaction tube types toward crystallization of cobalt (II) oxalate in gel. The gel was prepared at pH 5 by reacting sodium metasilicate solution with dilute nitric acid (for U-tube) and oxalic acid (for straight tube), with gelling time of 4 days and crystal growth time of 8 (for straight tube) and 12 (for U-tube) weeks. Result shows that pink crystalline powder was directly formed using straight tube method. The use of different solvents in straight tube method affects crystallization and could delay direct precipitation of the product. In contrast, bigger and better shape of red block crystal was yielded from U-tube method; however, longer growth time was needed. FTIR studies suggest that both growth method produces identical compound of hydrated cobalt (II) oxalate. © 2013 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)
Comparative Study of Various Preparation Methods of CuO–CeO2 Catalysts for Oxidation of n–Hexane and iso–Octane
Authors: Mishra A. , Tripathi B. D. , Rai A. K. , Prasad R. 
Published: March 2013

The complete oxidation of n-Hexane and iso-Octane was studied individually in a fixed bed tubular flow reactor over CuO-CeO2 catalysts synthesized via four different methods namely urea-nitrate combustion method, urea gelation/co-precipitation method, citric acid sol-gel method and co-impregnation method. Laser diffraction was employed in catalysts characterization. The results obtained from the complete conversion of n-Hexane and iso-Octane revealed that the CuO-CeO2 catalysts prepared by urea-nitrate combustion method (UNC) showed the best performance than the catalysts prepared by other methods used in the present investigation. CuO-CeO2 catalysts prepared by UNC method achieve total n-Hexane and iso-Octane conversion to CO2 at lower temperatures of 280 0C and 340 0C respectively due to the larger surface area of the catalysts which increases the specific rate of reaction. © 2013 by Authors, Published by BCREC Group. This is an open access article under the CC BY-SA License (https://creativecommons.org/licenses/by-sa/4.0)
A De Novo Deletion at 16q24.3 Involving ANKRD11 in a Japanese Patient With KBG Syndrome
Authors: Miyatake Satoko , Murakami Akira , Okamoto Nobuhiko , Sakamoto Michiko , Miyake Noriko , Saitsu Hirotomo , Matsumoto Naomichi 
Published: March 2013

AbstractKBG syndrome is a rare autosomal dominant congenital syndrome comprising developmental delay with various neurological involvements, macrodontia of the upper central incisors, characteristic facial dysmorphism, and skeletal anomalies. ANKRD11 was recently identified as the gene responsible for this syndrome. To date, there have been only five KBG syndrome families described, each carrying a single base substitution or a 1‐ to 14‐bp deletion of this gene. Here, we present a patient with clinically confirmed KBG syndrome carrying a de novo 690‐kb deletion at 16q24.3 involving part of ANKRD11. He had characteristic facial appearance, macrodontia of the upper central incisors, hand anomalies, delayed bone age and intellectual impairment without autistic features. Interestingly, the deleted region overlaps with the critical region for 16q24.3 microdeletion syndrome. We discuss the clinical entities of KBG syndrome and 16q24.3 microdeletion syndrome from a clinical and genetic point of view. © 2013 Wiley Periodicals, Inc.
Manganese‐Catalyzed Cross‐Coupling of Thiols with Aryl Iodides
Authors: Liu Tsung‐Jui , Yi Chih‐Lun , Chan Chien‐Ching , Lee Chin‐Fa 
Published: March 2013

AbstractA manganese‐catalyzed cross‐coupling reaction of thiols with aryl iodides, furnishing aryl thioethers in good to excellent yields has been reported; the system shows good functional group tolerance and enables the sterically demanding aryl iodides to couple with thiols.
Characterization of three loci for homologous gene targeting and transgene expression
Authors: Eyquem Justin , Poirot Laurent , Galetto Roman , Scharenberg Andrew M. , Smith Julianne 
Published: March 2013

AbstractIntegrative gene transfer is widely used for bioproduction, drug screening, and therapeutic applications but usual viral methods lead to random and multicopy insertions, contribute to unstable transgene expression and can disturb endogenous gene expression. Homologous targeting of an expression cassette using rare‐cutting endonucleases is a potential solution; however the number of studied loci remains limited. Furthermore, the behavior and performance of various types of gene cassettes following gene targeting is poorly defined. Here we have evaluated three loci for gene targeting, including one locus compatible with the proposed Safe Harbor criteria for human translational applications. Using optimized conditions for homologous gene targeting, reporter genes under the control of different promoters were efficiently inserted at each locus in both sense and antisense orientations. Sustainable expression was achieved at all three loci without detectable disturbance of flanking gene expression. However, the promoter, the integration locus and the cassette orientation have a strong impact on transgene expression. Finally, single targeted integrations exhibited greatly improved transgene expression stability versus multicopy or random integration. Taken together, our data suggest a potential set of loci for site‐specific transgene integration, suitable for a variety of biotechnological applications. Biotechnol. Bioeng. 2013; 110: 2225–2235. © 2013 Wiley Periodicals, Inc.
Improvement of ethanol productivity and energy efficiency by degradation of inhibitors using recombinant Zymomonas mobilis (pHW20a‐fdh)
Authors: Dong Hong‐Wei , Fan Li‐Qiang , Luo Zichen , Zhong Jian‐Jiang , Ryu Dewey D. Y. , Bao Jie 
Published: March 2013

AbstractToxic compounds, such as formic acid, furfural, and hydroxymethylfurfural (HMF) generated during pretreatment of corn stover (CS) at high temperature and low pH, inhibit growth of Zymomonas mobilis and lower the conversion efficiency of CS to biofuel and other products. The inhibition of toxic compounds is considered as one of the major technical barriers in the lignocellulose bioconversion. In order to detoxify and/or degrade these toxic compounds by the model ethanologenic strain Z. mobilis itself in situ the fermentation medium, we constructed a recombinant Z. mobilis ZM4 (pHW20a‐fdh) strain that is capable of degrading toxic inhibitor, formate. This is accomplished by cloning heterologous formate dehydrogenase gene (fdh) from Saccharomyces cerevisiae and by coupling this reaction of NADH regeneration reaction system with furfural and HMF degradation in the recombinant Z. mobilis strain. The NADH regeneration reaction also improved both the energy efficiency and cell physiological activity of the recombinant organism, which were definitely confirmed by the improved cell growth, ethanol yield, and ethanol productivity during fermentation with CS hydrolysate. Biotechnol. Bioeng. 2013; 110:2395–2404. © 2013 Wiley Periodicals, Inc.