bluuebunny/crossref_metadata_2025_split huggingface dataset:
Survey of the Prognosis for Dermatomyositis, with Special Reference to Its Association with Malignancy and Pulmonary Fibrosis
Authors: Hidano Akira , Kaneko Kayoko , Arai Yohko , Kikuchi Rika 
Published: April 2015

AbstractWe sent questionnaires to the major dermatological institutions in Japan concerning the course and associations of dermatomyositis (DM). Of 637 cases observed between 1973 and 1983, 68 were children. The mortality rate was 2.9% in children, 47.1% in adult males, and 30.0% in adult females. Associations with malignancies were noted in 30.1% of adults; 112 cases (19.7% of adults) died from these, but surgery improved the signs of DM in 22 cases. The main neoplasms were cancer of the stomach, lung, colon, and breast. Association with pulmonary fibrosis (PF) was observed in 85 cases (14.9% of adults) and, in spite of intensive treatment, 38 cases (6.7%) died from it. In the majority of PF cases who died within 2 months, pulmonary symptoms started within 6 months of the onset of DM. All cases of PF which occurred before the onset of DM survived. The concurrence of PF and malignancies was exceptional. Death from myocardial disease occurred in only 3 cases.
A Monocyte‐Aggregating Factor in the Sera of Patients with Sarcoidosis
Authors: Baba Toru , Ochiya Takahiro , Yamaguchi Koji , Baba Akiko , Uyeno Kenichi 
Published: April 2015

AbstractSarcoidosis sera were shown to have higher activity in aggregating normal human monocytes than normal human sera. A kinetic study suggested that monocytes may not be sensitive to the aggregating activity of sarcoidosis serum until they develop to some extent into macrophages. Gel filtration of sarcoidosis sera on Sephadex G‐200 showed that the major factor responsible for monocyte aggregation had the molecular weight of about 130,000. This major aggregating factor was unable to increase cell size of normal human monocytes or to increase phagocytosis and glucose consumption of normal human monocytes. It did have the ability to form multinucleate giant cells.
Protein AL Origin in Amyloidosis Cutis Nodularis Atrophicans
Authors: Masuda Chieko , Hayashi Masayuki , Kameda Yo , Mohri Shinobu , Nakajima Hiroshi , Nagai Ryukichi 
Published: April 2015

AbstractA case of amyloidosis cutis nodularis atrophicans (ACNA) was reported. The characteristics of the amyloid deposit were examined using the unlabeled immunoperxoidase method (peroxidase‐antiperoxidase method, so called PAP). The study revealed that the amyloid deposit contained A type protein AL, and the surrounding plasma cells contained λ and κ types of both immunoglobulin light chains and Bence Jones protein, with strong preference toward the λ type of each protein. These results suggest that, in ACNA, the plasma cells produce and secrete immunoglobulin light chains and/or Bence Jones protein, and that the protein degenerates into protein AL and then accumulates in the tissue to form nodules. ACNA seems to be a localized form of AL type amyloidosis, since patients with ACNA have no signs of systemic involvement.
Analyses of δ‐Aminolevulinic Acid Dehydratase Activity and Protoporphyrin Levels in Erythrocytes among Three Different Species
Authors: Murayama Fumio , Nonaka Shigeo , Ohgami Taro , Yoshida Hikotaro 
Published: April 2015

AbstractDelta‐aminolevulinic acid dehydratase (ALA‐D) is a enzyme that participates in the pathway from δ‐aminolevulinic acid (ALA) to porphobilinogen (PBG). This enzyme is relatively easy to analyze because erythrocytes can be utilized as the sample. However, it is not completely understood what kinds of abnormality in porphyrin metabolism influence ALA‐D activity, and it has few clinical applications.To elucidate the regulation of δ‐aminolevulinic acid dehydratase (ALA‐D) activity, we compared the ALA‐D activity and protoporphyrin levels in erythrocytes among three different species. The values for erythrocyte ALA‐D activity were highest in the guinea pig, followed by man and the mouse. Erythrocyte protoporphyrin levels showed a similar tendency. A definite correlation exists between ALA‐D activity and protoporphyrin levels in erythrocytes. From this data, it is speculated that ALA‐D activity in normal metabolism is a marker of heme synthesis.
Radiodermatitis
Authors: Okazaki Michiharu , Kikuchi Ichiro , Narita Hiromi , Ogata Katsumi , Inoue Shouhei 
Published: April 2015

AbstractBetween November of 1977 and April of 1985, 15 cases of acute radiodermatitis and 28 cases of chronic radiodermatitis were studied at Miyazaki Medical College. All cases of acute radiodermatitis were preceded by malignancies and radiotherapy; 17 cases of chronic radiodermatitis were seen after radiotherapy for benign conditions and 7 cases were seen after radiotherapy for malignant conditions. The other 4 cases of chronic radiodermatitis were of occupational origin. Chronic radiodermatitis studied histologically in 23 cases revealed the following findings; atypia in the epidermis in 9 cases (poikilodermatous lesions showed the least atypia), intraepidermal carcinoma in 6 cases, squamous cell carcinoma in 7 cases, and malignant fibrous histiocytoma in one case.
Amikacin Treatment for Mycobacterium Marinum Infection
Authors: Arai Hiroko , Nakajima Hiroshi , Naito Shizuo , Kaminaga Yoichiro , Nagai Ryukichi 
Published: April 2015

AbstractThis report describes a case of Mycobacterium marinum skin infection. A granulomatous plaque on the dorsum of the left hand of a 71‐year‐old man who kept a tank of tropical fish was followed by erythema and induration on the left forearm of the lymphocutaneous type. The lesion was successfully treated with 6 weeks of administration of amikacin (28 intramuscular injections) a total of 3500 mg. The isolated M. marinum strain was sensitive in vitro to amikacin at 0.78 μg/ml. Furthermore, another 15 clinical strains of M. marinum showed high sensitivity in vitro to this drug (93% of the 16 strains were sensitive to between 0.78 and 1.56 μg/ml).
Low Glucose‐6‐Phosphate Dehydrogenase Activity in Cultured Skin Fibroblasts from Werner's Syndrome
Authors: Shindo Yasuko , Akiyama Junichi , Matsumoto Kazuhiko , Takase Yoshio , Hashimoto Takashi 
Published: April 2015

AbstractFibroblasts from leg skin of a patient with Werner's syndrome were cultured. Their growth rate was lower than that of the normal control. They were variable in size and did not reach confluence. The activity of glucose‐6‐phosphate dehydrogenase (G6PDH) in this cell line was one seventh that of the normal control. The results of pulse labeling and chase experiments indicated that the rate of synthesis of G6PDH in the line was low, in agreement with the decrease in enzyme quantity.
Successful Treatment of Cheilitis Granulomatosa with Tranilast
Authors: Kato Taizo , Tagami Hachiro 
Published: April 2015

AbstractA new anti‐allergic drug, tranilast [N‐(3′,4′‐dimethoxycinnamoyl) anthranilic acid] was used on a 46‐year‐old Japanese female patient with cheilitis granulomatosa. A swelling of upper lip with repeated recurrence every two weeks was dramatically suppressed by treatment for 3 months. After drug withdrawal, she was free of symptoms for 12 months. The mode of action of tranilast in cheilitis granulomatosa is not understood at the present time.
Ultrastructural Observations of Skin Lesions in MRL Mice —Dermal Infiltrations and Capillary Changes
Authors: Horiguchi Yuji , Furukawa Fukumi , Imamura Sadao 
Published: April 2015

AbstractThe skin lesions of MRL/l mice were examined by electron microscopy and the following changes were disclosed in the dermis. Fibroblasts and mast cells were increased in number and activated. A major part of the infiltrating mononuclear cells was morphologically T‐lymphocytes. The capillaries, degenerated to varying degrees with indistinct basal laminae, were remarkably increased, and exudative amorphous material was deposited around them. Neither electron‐optically dense deposits around the vessels suggesting immune deposits nor tubulo‐reticular structures in the endothelial cells were detected.The basic lesions occurring in the dermis of MRL/l mice seem to be primary injuries of the capillary endothelial cells and consequential exudative changes of perivascular areas, similar to those of erythematous lesions in human systemic lupus erythematosus.
Basal Lamina of the Dermal Capillary: Concentric Multilamination Following Regeneration Processes
Authors: Imayama Shuhei , Ikawa Tomoko , Urabe Harukuni 
Published: April 2015

AbstractConcentric multilamination of the basal lamina around the subepidermal capillaries was observed after reaction to dinitrochlorobenzene. It consisted of luminal obstruction, necrosis of endothelial cells and pericytes, regeneration of these cells, and new deposits of basal lamina. Beneath the two basal laminae, delicate collagenous fibrils were present. These findings help explain the similar but more pronounced lamination of the vascular basal lamina in patients with diabetes, protoporphyria, etc. and the endothelial production of fibrillar collagen under certain condition.
A Comparative Immunohistochemical Study of Adult T Cell Leukemia and Cutaneous T Cell Lymphoma
Authors: Sudo Shigenori , Morohashi Masaaki 
Published: April 2015

AbstractAn immunoperoxidase study of 2 cases of adult T cell leukemia (ATL) and 2 cases of mycosis fungoides (MF) was done using monoclonal antibodies. In ATL, many anti‐interleukin‐2 receptor antibody (LeuIL‐2R)‐positive cells were seen in the dermis and occasionally in the epidermis. In contrast, in MF, LeuIL‐2R‐positive cells were much less frequent. LeuIL‐2R‐positive cells in MF may be non‐malignant T cells; not all LeuIL‐2R‐positive cells may be malignant in ATL. These non‐malignant LeuIL‐2R‐positive cells, we suggest, are involved in the interaction between malignant T cells and reactive infiltrating cells. Furthermore, in addition to OKT6‐positive cells, OKM1‐positive cells were seen in the infiltrates in the dermis in both ATL and MF. OKM1‐positive cells also participate in the mechanism of the skin affinity in ATL and cutaneous T cell lymphomas.
Diabetic Retinopathy and Campbell de Morgan's Spots
Authors: Shah Kirtikant C. 
Published: April 2015

AbstractA comparative study of de Morgan's spots in one hundred diabetics with retinal microangiopathy, one hundred diabetics without retinopathy, and one hundred normal persons showed a definite relationship between microangiopathy in retina and macroangiopathy in the form of de Morgan's spots on the skin of uncontrolled diabetic patients. The frequency of cases with de Morgan's spots on the skin was significantly higher in diabetics with retinal microangiopathy at all ages irrespective of sex than that in diabetics without retinal microangiopathy or normal persons. The spots were most numerous and prominent in patients with retinal microangiopathy.
Surgical Treatment of Speckled Skin Caused by Dyschromatosis Symmetrica Hereditaria
Authors: Taki Tadashi , Kozuka Sadao , Izawa Yohei , Hiramatsu Mitsuyoshi , Usuda Toshikazu , Yokoo Kazuhisa , Matsuda Toshiki , Kou Tomihiko , Kobayashi Taisuke 
Published: April 2015

AbstractThin split skin autografts taken from the abdomen were used to replace speckled skin caused by dyschromatosis symmetrica hereditaria in one patient. Normal skin colour appeared in the recipient areas and no recurrence was observed in the treated areas.
Pseudo‐Kaposi Sarcoma with a Possible Relationship to Obesity
Authors: Hatakenaka Ken‐ichi , Arata Jirô , Masuda Toshiki 
Published: April 2015

AbstractA 27‐year‐old man had pseudo‐Kaposi sarcoma on his right lower leg. Angiography showed that hypervascular changes were present at the site of skin lesions. No gross arteriovenous fistula was seen. Development of the disease in our patient seemed to be related to obesity (height 167.8cm, body weight 74 kg). Reduction of his body weight by diet has been most effective for his lesions.
Annular Elastolytic Giant Cell Granuloma: An Unusual Case with Lesions Arising in Non‐sun‐exposed Areas
Authors: Muramatsu Tsutomu , Shirai Toshihiko , Yamashina Yukio , Sakamoto Kuniki 
Published: April 2015

AbstractA 70‐year‐old man with a 2‐year history of annular elastolytic giant cell granuloma associated with diabetes mellitus was reported. The lesions mainly developed in non‐sun‐exposed areas. Histologic examination revealed phagocytosis of elastic fibers by histiocytic cells. Immunoperoxidase staining for lysozyme disclosed positive reactivity within the cytoplasm of these histiocytic cells. Electron microscopic study also showed elastic fibers and numerous lipid‐like substances in the cytoplasm of these cells. These findings indicate high phagocytolytic activity by these infiltrating cells. In our case, actinic damage was not considered to be a primary causative factor, and a possible pathogenesis was also discussed.
Surgical Treatment of a Deep Fungal Infection of the Skin by Trichosporon cutaneum
Authors: Seki Yoshihito , Otsuka Fujio , Ohara Kuniaki , Takizawa Kiyohiro , Ishibashi Yasumasa 
Published: April 2015

AbstractWe treated a patient with facial granuloma caused by Trichosporon cutaneum, in whom various therapeutic modalities had been ineffective. A surgical procedure combining excision, topical chemotherapy of the open wound, and covering the defect with a cervicofacial flap was finally successful.
Primary Osteoma Cutis
Authors: Nakamura Shinichi , Imai Toshiya , Nakayama Keiji , Onda Shutaro 
Published: April 2015

AbstractA 13‐month‐old male infant with primary osteoma cutis was reported. There was no diagnostic sign of Albright's hereditary osteodystrophy in either this patient or his family. Laboratory investigations showed no abnormalities except moderate obesity (kaup index=22).
Fibrous Proteins of Trichilemma‐oid Epidermal Keratinization without Keratohyalin Granules Formation
Authors: Tezuka Tadashi , Takahashi Masae 
Published: April 2015

AbstractInteractions between the histidine‐rich protein of keratohyalin granules and keratin fibers were investigated. Keratin fibers of the stratum corneum from sole skins of both normal persons (NP) and those with hereditary palmoplantar keratoderma (HPPK) were examined. HPPK samples showed trichilemma‐oid keratinization except at the sweat duct. Epidermis around the sweat duct showed stratum granulosum formation. The keratin pattern was lost in the non‐sweat duct region; any changes in the size and distribution‐pattern of keratin fibers were, however, observed by electron microscopy. In sodium dodecylsufate polyacrylamide gel electrophoresis (SDS‐PAGE), keratin fibers in the HPPK stratum corneum, from both non‐sweat duct and sweat duct regions, and in the stratum corneum of the sole lesion of the HPPK's mother, lacked 57kd subunits, but the electrophoretic profiles of other keratin subunits were identical to those from NP samples. It was concluded that the lack of histidine‐rich protein formation causes no change in keratin fibers themselves either under electron microscopy or in SDS‐PAGE.The absence of a 57kd subunit keratin fiber may have been caused by abnormalities in the production or degeneration processes of keratin fibers of the HPPK epidermis.
Fine Structural Arrangement of Fibrous Stroma around Dermal Capillaries
Authors: Imayama Shuhei , Yashima Yutaka , Urabe Harukuni 
Published: April 2015

AbstractCapillaries of the rat skin were sectioned in planes parallel, perpendicular, and oblique to the vascular long axis for the purpose of studying the perivascular fibrous stroma. Fine structural observation revealed that the vessels had organized stromal coats. Delicate collagen fibrils were regularly arranged in alternate, largely orthogonal layers pursuing a spiral or longitudinal course at the innermost layer. However, the fibrillar density and layering differed between the continuous and fenestrated segments of the capillary course. Along segments with a narrow lumen and a thick endothelium, the fibers were dense and both the longitudinal and circular layers exhibited almost complete turns around the vascular perimeter. The fenestrated segments, in contrast, showed a loosely arranged fibrillar coat and sometimes lacked the circular layer.
PNA‐binding Sites in Sebaceous Tumors
Authors: Tamaki Kunihiko , Ohara Kuniaki , Ishibashi Yasumasa 
Published: April 2015

AbstractThe presence and distribution of peanut agglutinin (PNA)‐binding sites was studied in normal sebaceous glands, nevus sebaceous, senile sebaceous hyperplasia, sebaceous adenoma, sebaceous epithelioma, and sebaceous carcinoma. Cell surface staining and sponge‐like cytoplasmic staining was observed in sebaceous glands, nevus sebaceus and sebaceous hyperplasia. In sebaceous adenoma, sebaceous epithelioma, and sebaceous carcinoma, diffuse cytoplasmic staining was observed in addition to cell surface staining.
Eosinophil Chemotactic Activity of β‐galactosidase
Authors: Isoda Midori 
Published: April 2015

AbstractThe chemotactic activity of β‐galactosidase from bovine liver for eosinophils was evaluated in vitro and in vivo. In vitro, the activity increased in a dose‐dependent manner. The maximal activity was detected at the concentration of 0.1 mg/ml. In vivo, at concentrations greater than 0.5 mg/ml, eosinophil chemotactic responses were clearly seen.These results suggest that β‐galactosidase, one of the acid hydrolases, may play a role in eosinophil accumulation in various inflammatory lesions.
T‐cell Subsets and NCI (Nuclear Contour Index) of Their Skin Infiltrates in Mycosis Fungoides (MF)
Authors: Zhang Kang Guo , Iwahara Kunio , Hantanyapong Yaowalak , Kiyohara Akira , Yaguchi Hitoshi , Ogawa Hideoki 
Published: April 2015

AbstractACNU solution was applied to plaque lesions once daily for 4 weeks, while 3 million IU/day of Interferon α was injected locally into tumor lesions for 4 weeks. Clinically, ACNU and Interferon α were very effective. To determine the cytological effectiveness of both treatments, T‐cell subsets and the NCI of their skin infiltrates in mycosis fungoides (MF) were compared pre‐ and post‐treatment. Using either ACNU and Interferon α, in pre‐treatment skin tissue, Leu3a positive cells were dominant, whereas in post‐treatment skin tissue, Leu2a positive cells were dominant. As a result, the helper/suppressor T‐cell (H/S) ratio in plaque lesion (3.1) was remarkably decreased (0.44) by ACNU treatment and the H/S ratio in tumor lesions was also reduced from 9.3 to 2.6 after injection of Interferon α. Moreover, the NCI of T‐cells in plaque lesions decreased from 7.57 ± 1.71 to 4.30 ± 0.75 following treatment with ACNU, and that in tumor lesions was also reduced from 8.62 ± 1.89 to 4.35 ± 1.17 with Interferon α treatment. These immunohistos‐chemical staining patterns and immunoelectron microscopic features in post‐treatment periods are similar to those of control skin tissue which is not malignant. It was of interest that the antiproliferative effect of Interferon α on Hut 78 cells (Sézary cells) in vitro was demonstrated. Therefore, Interferon α might have direct cytotoxic effects on MF cells similar to that with Sézary cells. These results indicate that both treatments, ACNU and Interferon α, are effective not only clinically but also cytologically. The data also indicate that the NCI of T‐cell and T‐cell subsets might be used as an indicator of a treatment's effectiveness against MF.
Hyperthermic Effects on Peripheral Lymphocytes Isolated from a Chronic Lymphocytic Lymphoma/Leukemia Patient In vitro
Authors: Nakayama Juichiro , Yasumoto Shinichiro , Kabemura Mayumi , Imayama Shuhei , Asahi Masakazu , Urabe Harukuni 
Published: April 2015

AbstractEffects of hyperthermia in vitro on the atypical lymphocytes isolated from peripheral blood of a chronic lymphocytic lymphoma/leukemia patient were investigated. Incubation of the isolated lymphocytes at 43°C for 5 hr killed 15% of the cells, while more than 95% of the lymphocytes isolated from normal individuals and heated under the same conditions survived. Cultivation of the patient's lymphocytes at 37°C for 4 days after heat treatment at 43°C for 5 hr resulted in a viability of less than 50%. Proliferative responses of the patient's lymphocytes to lectins showed 70% more inhibition than did the control when heated at 43°C for 1 hr and were abolished completely after 3 hr of hyperthermia. Although, in normal individuals, complete recovery of (3H)‐thymidine uptake of the lymphocytes heated at 43°C for 3 hr was observed after 4 days of cultivation at 37°C, this recovery did not occur in the patient's cells. Morphological changes in the atypical lymphocytes heated at 43°C for 3 hr occurred in the cytoplasmic organelles; in particular, enlargement of mitochondria, increased numbers of cytoplasmic granules and vesicles, probably derived from lysosomes, and hyperproduction of myelin sheath‐like structures around the nuclear membrane were seen.
Analysis of the In vitro Concanavalin A Response of Mouse Regenerating Spleen Cells after Cyclophosphamide Treatment
Authors: Sato Masahiro , Ikezawa Zenro 
Published: April 2015

AbstractThe effect of in vivo cyclophosphamide (Cy) treatment on the in vitro concanavalin A (ConA) response of murine spleen cells was investigated. The ConA dose‐response of the spleen cells in their regenerating phase—7 days after an injection of a sublethal dose (200 mg/kg) of Cy—was significantly different from that of normal spleen cells (N‐SCs); the high‐dose ConA response remarkably increased and the low‐dose ConA response decreased in the spleen cells (Cy‐SCs) taken 7 days after Cy treatment The phenotype of the responder cells in the ConA response of Cy‐SCs was Thy‐1+ and Lyt‐l+2−, the same T subset involved in the ConA response of N‐SCs. Removal of glass wool‐adherent cells from the N‐SCs did not have the same effect as Cy treatment on the ConA dose‐response, and addition of N‐SCs did not suppress an increased response of the Cy‐SCs to high‐doses of ConA.These results indicate that this alteration in the ConA dose‐response induced by in vivo Cy treatment cannot be explained by a decrease in the number of Cy‐sensitive regulatory cells. However, although the ConA response of Cy‐SCs was not affected either by depletion of Sephadex G‐10 adherent cells with a G‐10 column or by depletion of Ia positive cells with anti‐Ia plus complement‐mediated lysis, the ConA response of N‐SCs was inhibited remarkably by both procedures. Furthermore, addition of anti‐Ia antibodies to the cultures decreased the ConA response of N‐SCs, but did not affect the ConA response of Cy‐SCs. Apparently, Lyt‐1+2−T cells in the regenerating phase (on day 7) after Cy treatment required high doses of ConA, but neither the Ia molecule itself nor the Ia+ G‐10 adherent accessory cells (ACs) for maximum stimulation. Therefore, the change in ConA dose‐response induced by Cy treatment on day 7 is thought to be caused by alteration of ConA responsiveness of the regenerating Lyt‐l+2−T cells rather than by a decrease in the Cy sensitive regulatory cells.
Mating Behaviour of Microsporum canis from Human Ringworm Cases in Hokkaido Prefecture in Japan
Authors: Kubo Hitoshi , Iizuka Hajime , Shibaki Hideomi 
Published: April 2015

AbstractTwo‐hundred and seventy‐nine isolates of Microsporum canis obtained from Japanese human ringworm cases in Hokkaido prefecture during the past 5 years were paired with either the Nannizzia otae (+) or (–) tester strain by the standard hair soil plate technique. Among the 279 M. canis isolates, 257 were (–) mating type, 8 were (+) and 14 were nonreactive. Our results are in accord with the current view that the predominant strain of M. canis in Japan (or in the world) is the (–) mating type strain. However, contrary to the previous assumption, the (+) mating type strain was not as rare as expected (3% of the total successful mating strains), at least in Hokkaido prefecture.The N. otae (+) strain might also have an unequal geographic distribution, since 10 out of the 12 isolates of the N. otae (+) strain so far reported have originated from Hokkaido prefecture.