bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blame Attribution, Partisanship, and Federalism: Evidence from a Panel Survey
Authors: Uttermark Matthew J , Mewhirter Jack , Sanders Rebecca , McLaughlin Danielle M 
Published: February 2025

Abstract
When disaster strikes in federal systems, who do citizens blame and why? Partisan blame attribution (PBA) suggests that Republicans and Democrats hold consistent ideological preferences on which level of government should respond and receive blame for policy failures in times of crisis. Conversely, partisan federalism (PF) suggests that partisans will opportunistically assign blame to the level of government controlled by their disfavored party. Research on blame attribution has yet to assess which mechanism better explains patterns of blame attribution among individuals. We test each mechanism to evaluate patterns of blame attribution related to the COVID-19 pandemic. Leveraging panel survey data collected before the 2020 election and after the 2021 presidential transition, we find that Democrats uniformly follow PF expectations, whereas Republicans follow both PBA and PF expectations. We conclude that scholars should revisit blame attribution and more carefully consider the role of federalism in determining citizen preferences.
The Rhetorical “What Goes with What”: Political Pundits and the Discursive Superstructure of Ideology in US Politics
Authors: Green Jon 
Published: February 2025

Abstract
In his foundational essay, Philip Converse (1964) alludes to a small class of political sophisticates who are influential in the social construction and diffusion of ideological belief systems. However, despite their importance to the nature of ideology in the public at large, there is little empirical research focusing on those select few involved in this process of “creative synthesis”—and work examining the rhetorical structures that cohere sets of plausibly independent issue stances is rarer still. I do so here using a novel dataset of tweets sent by roughly 1,000 of the most prominent political pundits in the United States from January through August of 2019. Using a combination of text and network-analytic techniques, I identify robust relationships between both how pundits discuss a wide range of political concepts and the extent to which this discussion is informative of their network-based preferences. I find that broad, organizing concepts such as partisan and ideological labels emerge as central in political discourse, facilitating indirect links between a wide range of issues. However, I also find that discussions of discrete policy issues and political concepts are highly informative of pundits’ latent preferences. This descriptive account of the discursive superstructure of ideology offers new evidence regarding how creative synthesis is conducted in practice.
Potential drug interactions with nirmatrelvir/ritonavir in critically ill patients with COVID-19 – a retrospective observational study
Authors: Jarczak Dominik , König Christina , Röhr Anka C , Forstreuter Anika , Brehm Thomas Theo , Schulze zur Wiesch Julian , Roedl Kevin , Kluge Stefan , Fischer Marlene 
Published: February 2025

Abstract

Objectives
Nirmatrelvir/ritonavir is recommended for high-risk patients with COVID-19 to reduce disease progression and mortality. Ritonavir significantly increases the bioavailability of nirmatrelvir and is the most potent irreversible cytochrome P 450 3A4 inhibitor in clinical use, resulting in a substantial risk for drug-drug interactions (DDI). We aimed to analyze the incidence of potential DDI (pDDI) in critically ill patients with SARS-CoV-2 infection.

Methods
This is a retrospective single-center study in a quaternary care center in Northern Germany. We reviewed electronic health records for demographic characteristics, comorbid conditions, and medication history. The pre-existing comedication was screened for pDDI with nirmatrelvir/ritonavir using publicly available databases. Binary logistic regression was used to identify patient characteristics associated with pDDI.

Key Findings
Of 500 critically ill patients with SARS-CoV-2 infection, 362 (72.4%) received pre-existing comedication. A total of 241/500 patients (48.2%) had a medication history prone to pDDI. Antidiabetics, lipid-lowering drugs, and anticoagulants were among the most frequently used agents with a pDDI. Higher age (OR 1.043; 1.028-1.058; p&lt;0.01) and the number of comorbidities (OR 1.229; 1.119-1.350; p&lt;0.01) were significantly associated with pDDI.

Conclusion
The very patient population that may benefit most from treatment with nirmatrelvir/ritonavir also has the greatest risk of pDDI. Polypharmacy is frequently present in these patients and a conscientious check of the comedication is mandatory before a treatment with nirmatrelvir/ritonavir can be initiated.
Key drivers of innovation trajectories and catch-up in latecomer firms: absorptive capacity and opportunity capture
Authors: Petti Claudio , Hu Xinyue , Tang Yongli , Schiuma Giovanni 
Published: February 2025

Abstract
This paper investigates why some latecomer firms with substantial absorptive capacity fail during catch-up phases. It aims to clarify the performance effects of their incremental versus radical innovation trajectories propelled by potential and realized absorptive capacities and the interplay with opportunity capture. The empirical investigation was conducted with a sample of 166 manufacturing firms in Guangdong Province, China. The results indicate that both potential and realized absorptive capacities and opportunity capture are more effective in enhancing the performance of incremental innovations than those of radical innovations. However, a high level of opportunity capture can diminish the beneficial impacts of absorptive capacity on radical innovation performance to a greater extent. These findings enrich extant literature on latecomer firms’ catch-up strategies by combining the theoretical lens of absorptive capacity and opportunity capture. It offers implications for managers and policymakers, guiding strategy and policy formulation for firm catch-up endeavors.
Surgical techniques in chondrolaryngoplasty: a narrative review
Authors: de Almeida Rodolfo B S , Costa Claudiney C , Ramos Hugo V L , e Silva Duarte Pauliana L , Velasco Leandro C , Cervantes Onivaldo 
Published: February 2025

Abstract

Introduction
Chondrolaryngoplasty, also known as “Adam’s apple” reduction, is a surgical procedure that diminishes the prominence of the thyroid cartilage. Primarily sought by transgender women, the procedure is performed for esthetic reasons to align with their identified gender. It can also be done for cisgender individuals seeking reduced prominence. Despite its safety, there are risks due to the proximity to vocal cords, and variations may impact results.

Objective
To identify the main techniques available in the literature for performing chondrolaryngoplasty, as well as the differences in the results of each technique applied.

Methods
Research utilized databases (MEDLINE, PubMed, and Scopus) from inception to March 2023, focusing on randomized clinical trials, prospective and retrospective studies, and case series published in English, Spanish, French, or Portuguese. Selected studies provided results including satisfaction rates, surgical time, and/or details on complications.

Results
21 eligible results, four of which were excluded because the full text was not available and four because they did not meet the research objectives. Thus, 13 articles were included in the analysis, eight of which reported on external approach surgical procedures (via surgical incision) and five on transoral procedures, totaling 355 living patients evaluated, as well as 11 cadavers. The complications reported are considered mild and transient, and do not contraindicate the procedure. Among the factors that most interfere with patient satisfaction is the apparent scar, which is avoided with the transoral surgical approach, which has been reported more recently.

Conclusion
Chondrolaryngoplasty is an esthetic surgical procedure that aims to reduce the prominence of the thyroid cartilage and is most commonly performed on transgender women. Studies show that it is a safe procedure, with rare complications that are generally transient. The main drawback of the surgery is the apparent scar which can be avoided by a transoral approach.
Global Patterns of Taxonomic Uncertainty and its Impacts on Biodiversity Research
Authors: Guedes Jhonny J M , Moura Mario R , Jardim Lucas , Diniz-Filho José Alexandre F 
Published: February 2025

Abstract
Over two million species have been named so far, but many will be invalidated due to redundant descriptions. Undetected invalid species (i.e., synonyms) can impair inferences we make in biodiversity research and hamper the implementation of effective conservation strategies. However, the processes leading to the accumulation of invalid names remain largely unknown. Using multi-model inferences, we investigated the patterns and potential drivers of species- and assemblage-level variation in synonym counts across terrestrial vertebrates globally. We also explored how taxonomic uncertainty (i.e., instability in species identities) can affect latitudinal variation of diversification rates. The average number of synonyms was higher for species described earlier, better represented in scientific collections, with larger geographic ranges, occurring in temperate regions, and in areas of high biodiversity attention. In assemblage-level models, a higher average number of synonyms was associated with temperate regions harbouring more early-described species. Areas of high endemism richness showed fewer synonyms across amphibians and reptiles but had an inverse effect for birds and mammals. Other predictor-response relationships varied across taxonomic groups, biogeographical realm, and spatial grain. Assuming that more synonyms indicate more stable species that have been thoroughly studied and reviewed, high synonym numbers in temperate species and assemblages support claims of a potential latitudinal taxonomy gradient, where geographic variation in taxonomic practice could hinder the proper recognition of tropical species. We show that the accumulation of invalid names is not random and discuss how invalid hidden names can affect biodiversity inferences. A potential approach to address this problem would be developing a taxonomic uncertainty metric that could be incorporated into models (i.e., as weights to account for varying degrees of uncertainty during the fitting process). Our study provides an initial approximation and highlights the often-neglected issue of uncertainty and instability in species identities from a macroecological perspective.
Molecular phylogeny of Salmoneus Holthuis, 1955 and its satellite genera (Decapoda: Caridea: Alpheidae) reveals hidden diversity and multiple instances of morphological and ecological convergence
Authors: Ashrafi Hossein , Anker Arthur , Ďuriš Zdeněk 
Published: February 2025

Abstract
The present study deals with the intricate phylogenetic relationships within an important clade of the caridean shrimp family Alpheidae, which includes the genera Salmoneus, Deioneus, and Triacanthoneus. Using molecular data from four genes (16S, 18S, COI, and H3), we herein provide the most comprehensive phylogenetic hypothesis for this alpheid clade, with 161 terminals representing 117 ESUs, i.e. genetically distinct species. Our analysis reveals four well-supported larger Clades, as well as 25 smaller clades, corresponding to species groups or species complexes. The results also show that the symbiotic lifestyle evolved multiple times within this clade, in some instances promoting convergent evolution. Ancestral character state analysis unveils extreme plasticity and independent shifts in the minor cheliped types. Furthermore, the impact of different lifestyles on the evolution of each minor cheliped type is discussed. Finally, we address the resulting paraphyly of Salmoneus and the intergeneric relationships between Salmoneus, Deioneus and Triacanthoneus.
Cell cycle‐based antibody selection for suppressing cancer cell growth
Authors: Song Chi Hun , Lin Chih‐Wei , Han Kyung Ho 
Published: February 2025

AbstractCell cycle arrest and programmed cell death are crucial biological processes in cancer development. Regulating cell fate decisions is essential due to their potential to induce cell cycle arrest and cell death. Inducing cell cycle regulatory proteins in tumor cells is considered a key objective in cancer therapy. Here, we present a novel method that selects antibodies from an antibody library to inhibit cancer growth using fluorescence‐activated cell sorting (FACS) assays and cell cycle analysis. This approach seeks antibodies that induce cancer cells to enter the G0 or G1 phase, a quiescent state where cells cease to proliferate and trigger programmed cell death. We found that the T1 antibody effectively suppresses the proliferation of cancer cells. Mechanistically, serine protease 3 (PRSS3) is a target antigen of the T1 antibody. We demonstrated that PRSS3 controls tumor cell proliferation and apoptosis through interaction with the T1 antibody. This research suggests that PRSS3 holds great potential as a target for solid cancer treatment. This cycle‐based approach to antibody screening shows potential because it can be broadly applied to cancer and other challenging diseases.
The Readability, Actionability, and Accessibility of Hemorrhoid-Focused Online Patient Education Materials: Are We Adequately Addressing Patient Concerns?
Authors: Eng Isabel K. , Chen Formosa , Russell Marcia M. , May Folasade P. , Labora Amanda , Salinas Daniela , Russell Tara A. 
Published: February 2025

BACKGROUND:
Hemorrhoidal disease is highly prevalent in the United States and frequently queried online. Unfortunately, health education webpages often lack reliable information.

OBJECTIVE:
To evaluate whether online hemorrhoid education materials in English and Spanish meet national recommendations for readability, actionability, and accessibility, and provide critical clinical guidance on when to seek medical care.

DESIGN:
Using three search engines (Bing, Google, Yahoo), we selected the top 30 results for formal medical and colloquial English and Spanish search terms regarding hemorrhoids. We assessed readability using validated scoring systems for readability in English and Spanish to report median reading levels and assessed Health Literacy Performance on a six-point checklist in three categories: accessibility, actionability, and critical clinical guidance.

SETTINGS:
University of California Los Angeles.

MAIN OUTCOME MEASURES:
Readability and health literacy performance.

RESULTS:
After removing duplicates, 90-95 webpages generated from formal English, Spanish, and colloquial English terms remained. There was minimal overlap of results from the formal and colloquial English searches. Median reading levels were first-year university for formal and colloquial English webpages, and eleventh grade for Spanish webpages. 43.2%, 48.4%, and 18.2% of formal English, Spanish, and colloquial English websites, respectively, had minimal Health Literacy Performance. Health Literacy Performance criteria that were met least often were printability and providing specific, actionable goals for patients to implement.

LIMITATIONS:
Our study represents searches completed at one point in time utilizing specific terms. Colloquial search terms were generated via survey with convenience sampling and may not be representative of all possible searches used by patients seeking information on hemorrhoidal disease.

CONCLUSIONS:
Most English and Spanish hemorrhoid-focused webpages failed to provide appropriate patient education, as they exceeded the recommended sixth-grade reading level, lacked actionable recommendations, were not accessible, and failed to provide critical clinical guidance. Online resources are essential for patients of all health literacy levels; improvement is critical to reduce healthcare disparities. See Video Abstract.
Validation and Sensitivity Analysis of the COVID-19 Transmission Model Simulating Counterfactual Infections in Japan
Authors: Kakeya Hideki , Umeno Yoshitaka 
Published: February 2025

AbstractBackgroundKayano et al. estimated the potential number of COVID-19 cases and deaths in Japan from February 17 to November 30, 2021, in a counterfactual scenario where no vaccination was implemented. Their model predicted 63.3 million cases and 364,000 deaths, with a claimed 95% confidence interval of less than 1% of the estimated values.ObjectiveTo validate the transmission model used by Kayano et al. by simulating infection counts in Japan during 2020 and assessing the impact of errors in the reproduction number of the Delta variant on infection count estimates in the counterfactual scenario.MethodsWe replicated the model used by Kayano et al. to simulate infection dynamics in Japan during 2020. We evaluated the model’s performance by comparing the simulated infection surges with actual data. Additionally, we analyzed the sensitivity of infection count estimates to ±10% errors in the reproduction number of the Delta variant, corresponding to the 95% confidence interval reported in the cited study.ResultsThe model successfully reproduced the first infection surge in early 2020, but it failed to replicate the second and third infection surges in the summer and winter of 2020. Sensitivity analysis revealed that a ±10% error in the reproduction number led to an estimated infection count error range of −25% to +42%.ConclusionThe results suggest potential limitations in the validity of the counterfactual simulation results presented by Kayano et al., particularly regarding the accuracy of infection count estimates and the confidence interval.
Chemical coupling of biological magnetosomes with amine-functionalized therapeutic antibody conjugates
Authors: Payan S.M , Gandarias L , Avellan R , Faivre D , Prévéral S 
Published: February 2025

ABSTRACTBackgroundImmunotherapy, particularly in cancer treatment, can be enhanced using antibody-drug or antibody-radionuclide conjugates. These conjugates disrupt cell signaling and induce cell death, requiring the targeted antigen to be highly expressed on tumor cells to avoid damage to healthy tissues. A promising strategy to improve delivery is the use of magnetosomes, biological magnetic nanoparticles, which can be guided to tumor sites using magnetic fields. However, most antibody-drug or antibody-radionuclide conjugates are functionalized using the antibody amine groups of the lysine residues on the heavy chains. Therefore, these amine groups are no longer available to bind the antibodies to the magnetosomes.ResultsHere, we explore an alternative approach to bind amine-functionalized antibodies to magnetosomes. Using SulfoSuccinimidyl-4-(N-Maleimidomethyl)Cyclohexane-1-Carboxylate (S-SMCC) as a crosslinker, the antibodies are chemically attached to the magnetosome membrane via thiol groups through antibody partial reduction. Our results demonstrate that this chemical process preserves the integrity and functionality of both magnetosomes and antibodies. Moreover, we prove that the produced magnetosome-antibody conjugates are stable under variousin vivo-like conditions.ConclusionThis coupling method offers significant advantages, enabling the coupling of therapeutic molecules to antibodies combined with the magnetic properties of magnetosomes. This new strategy aims to improve cancer therapy through targeted delivery and rapid accumulation at tumor sites.
Deep learning-based prediction of tissue-specific splice sites in the human neural retina
Authors: Riepe Tabea V. , de Bruijn Suzanne E. , Roosing Susanne , Cremers Frans P.M. , Hoen Peter A.C. ‘t 
Published: February 2025

AbstractSplice prediction tools can be used to identify splice-altering variants in patients with inherited diseases. Since splicing is tissue-specific, a predicted splice defect may vary depending on the tissue of interest. Current splice prediction tools often neglect splice junctions from the human neural retina, which can lead to the misinterpretation of benign and pathogenic variants in patients with inherited retinal diseases (IRDs). To address this issue, we developed a retina-specific splice prediction tool based on the model architecture of SpliceAI. In addition to retraining the existing model using splice junctions derived from retina RNA-sequencing data, we also improved the model’s generalization through hyperparameter optimizations such as dropout. The resulting retina-specific model was validated on retina-enriched exons and variants known to cause retina-specific splicing defects. Although the retina model correctly identified five more retina-enriched splice sites than the GTEx model, it did not enhance predictions for variants with tissue-specific splicing defects nor did it identify novel pathogenic variants in a set of genetically unexplained IRD cases. Despite these limitations, the retina-specific SpliceAI model shows promising results and should be applied to a larger patient cohort to uncover novel splice-altering variants in IRD patients.
Altered mitochondrial respiration is associated with loss of nuclear-encoded OXPHOS genes in parasitic broomrapes
Authors: Cai Liming , Jansen Robert K , Havird Justin C 
Published: February 2025

ABSTRACTParasitic plants, characterized by their dependency on host organisms for nutrients, have displayed far-reaching alterations in physiology and genetics. While significant gene losses and relaxed selection have been documented in the nuclear and plastid genomes, how parasitism impacts the molecular evolution and function of mitochondria has remained controversial. One of the main culprits hindering our understanding in this area is the lack of knowledge on nuclear-encoded mitochondrial-targeted genes (N-mt), which encode most mitochondrial oxidative phosphorylation (OXPHOS) proteins. By conducting a comprehensive survey of N-mt genes across angiosperms, we demonstrated significant gene losses and horizontal transfers associated with relaxed selection unique to holoparasitic Orobanchaceae. These putative losses and transfers have the potential to affect mitochondrial function directly and cause mitonuclear incompatibility because of breakdown between co-evolved protein complexes from mitochondrial and nuclear genomes. Our physiological assessments using high-resolution respirometry revealed that despite genetic alterations, holoparasitic Orobanchaceae maintained robust OXPHOS function but relied more on the fully nuclear-encoded succinate dehydrogenase (complex II). Our results document the first example of significant and biased gene loss in nuclear-encoded mitochondrial OXPHOS genes in parasitic plants, expanding on previous studies focused on mitochondrial-encoded genes and elucidating the mechanisms underlying the preservation of OXPHOS function despite genomic reduction.
Helix-8 and Carboxyl Terminal Tail Regulation of Proteinase Activated Receptor-4 (PAR4) signalling
Authors: Thibeault Pierre E. , Mousa Amr A.K. , Mirka Victor M. , Ramachandran Rithwik 
Published: February 2025

AbstractThe 8th-helix (H8) and carboxyl terminal tail (CT) of GPCRs are crucial for interactions with intracellular signaling molecules and regulatory proteins. We investigated how H8 and CT residues influence signaling in PAR4, a tethered-ligand activated GPCR. Our analysis revealed that PAR4 activation by thrombin or AYPGKF-NH2 stimulates β-arrestin-1/-2 recruitment and activates multiple Gα proteins (Gαq, Gα11, Gαz, Gα15, Gα12, Gα13, Gαi1-3, GαoA/B). Mutation of the H8 sequence (R352AGLFQRS359) significantly reduced G protein activation and β-arrestin recruitment, with residues Leu355-Glu357being particularly important. Mutations of specific lysine residues in H8 and CT also impaired signaling. Additionally, we identified a crucial TM7-H8 interaction and found that CT phosphorylation sites regulate β-arrestin recruitment. Finally, using AlphaFold3 we predicted interactions between PAR4, β-arrestins, and G proteins, revealing novel receptor regulatory sites in the intracellular loops, transmembrane domains, H8 and CT.