bluuebunny/crossref_metadata_2025_split huggingface dataset:
The co‐occurrence of two powdery mildew species on Cannabis sativa in the Czech Republic
Authors: Mieslerová Barbora , Kitner Miloslav , Šenkyřík Josef Baltazar , Křivánková Tereza , Sedlářová Michaela , Braun Uwe , Lebeda Aleš 
Published: June 2024

AbstractIn late summer 2022 and 2023, powdery mildew symptoms were found on female plants of hemp (Cannabis sativa) growing in a private garden in Hradec nad Moravicí (Silesia, Czech Republic). On the upper sides of the leaves, white powdery colonies were observed which coalesced and finally covered the whole leaf surface. The stems and flowers were not affected. Microscopic examination revealed the formation of asexual (anamorphic) states characterized by conidiophores forming catenescent conidia (Euoidium type), but with two types of conidia, viz., ellipsoid to ovoid or doliiform conidia with fibrosin bodies (resembling conidia of Podosphaera spp.), and ellipsoid to doliiform conidia without fibrosin bodies (resembling conidia of Golovinomyces spp.). Molecular genetic analyses confirmed co‐infections in the studied hemp samples by two powdery mildew species, viz., Podosphaera macularis and Golovinomyces ambrosiae, which represent first records from the Czech Republic, as well as Europe and in the world. The authors discuss the latest information and ideas regarding this pathosystem.
Relationship between stocking rate and production of stocker hybrid tilapia and water quality in a mixtotrophic biofloc system
Authors: Green Bartholomew W. , Rawles Steven D. , Ray Candis L. , McEntire Matthew E. 
Published: June 2024

AbstractIntensified production of stocker‐size tilapia can be accomplished using the biofloc technology production system. Stocker‐size tilapia (ca. 10–200 g/fish) are raised for subsequent stocking for grow‐out to market size. The relationship between stocking rate and the production of stocker‐size hybrid tilapia (Oreochromis aureus × O. niloticus) was evaluated in two studies in outdoor mixotrophic biofloc tanks. Hybrid tilapia were stocked in 18.6‐m2 (15.7‐m3) tanks at 50–150 fish/m2 (59–178 fish/m3) in 25 fish/m2 (30 fish/m3) increments in Study 1 and grown for 141–143 days. Stocking rates evaluated in 4.7‐m2 (3.6‐m3) tanks during Study 2 over 78–79 days ranged from 100 to 300 fish/m2 (132–396 fish/m3) in 25 fish/m2 (33 fish/m3) increments. Linear regression models best described growth to stocker size in both studies. Fish yield increased linearly with stocking rate and ranged from 20.1 to 38.3 kg/m3. Individual weight at harvest was inversely related to stocking rate and ranged from 389.8 to 163.4 g/fish and 155.2 to 81.4 g/fish in Studies 1 and 2, respectively. Partial budget analyses suggested positive net changes in profit with incremental increases in stocking rate for producing a 75‐g stocker. Water quality concentrations were impacted directly by daily feed ration and total feed fed, which increased linearly with stocking rate. In Study 2, whole‐body dry matter, protein, protein retention, and lipid were unaffected by stocking rate, whereas whole‐body ash increased with stocking rate. Whole‐body energy retention significantly decreased with stocking rate. The outdoor mixotrophic biofloc production system is well suited for intensive production of hybrid tilapia stockers. However, additional replicated research and economic analysis are needed to evaluate stocking rate and culture duration to produce specific‐sized stocker hybrid tilapia.
Urotherapy and biofeedback resistant dysfunctional voiding: How to deal with?
Authors: Dönmez M. İrfan , Selvi Ismail , Oktar Tayfun , Ziylan Orhan 
Published: June 2024

AbstractObjectivesTo analyze the management strategies in the children who had treatment‐resistant dysfunctional voiding (DV).MethodsAmong 75 children with DV who underwent pelvic floor biofeedback therapy (BF) between 2013 and 2020, 16 patients (14 girls, 87.5%) with a mean age of 9.81 ± 2.53 years that showed incomplete clinical response following urotherapy and initial BF sessions were retrospectively reviewed. The demographic and clinical characteristics, DVSS, and uroflowmetry parameters were recorded before and after the initial BF sessions. Subsequent treatments after initial BF and clinical responses of patients were noted.ResultsClinical success was observed in one patient by addition of an anticholinergic and in three patients with combination of salvage BF sessions and anticholinergics, whom had predominant overactive bladder (OAB) symptoms. The success rate of TENS alone and in combination with other treatment modalities was 88.8% (8/9 patients). In addition, salvage BF sessions (range 2 to 3) enabled clinical success in five (50%) of 10 cases as a combination with anticholinergics or TENS. In case of incomplete emptying without OAB, adequate clinical response to Botulinum‐A was observed during an average follow‐up of 29 months in two boys who did not respond to alpha‐blockers, even though one required repeat injection after 10 months. The total clinical success rate was 87.5% (14/16 patients) after a median follow‐up of 24 months. VV‐EBC and Qmax increased by a mean of 30.89% and 7.13 mL/min, respectively, whereas DVSS decreased by a mean of 8.88 points and PVR‐EBC decreased by a median of 19.04%.ConclusionsOur findings showed that clinical success in resistant DV was achieved by various combination treatments in the majority of children. However, a small group may still have persistent, bothersome symptoms despite multiple treatment modalities.
Proteomic profiling of polyglucosan bodies associated with glycogenin‐1 deficiency in skeletal muscle
Authors: Visuttijai Kittichate , Hedberg‐Oldfors Carola , Costello Daniel J. , Bermingham Niamh , Oldfors Anders 
Published: June 2024

AbstractAimsPolyglucosan storage disorders represent an emerging field within neurodegenerative and neuromuscular conditions, including Lafora disease (EPM2A, EPM2B), adult polyglucosan body disease (APBD, GBE1), polyglucosan body myopathies associated with RBCK1 deficiency (PGBM1, RBCK1) or glycogenin‐1 deficiency (PGBM2, GYG1). While the storage material primarily comprises glycans, this study aimed to gain deeper insights into the protein components by proteomic profiling of the storage material in glycogenin‐1 deficiency.MethodsWe employed molecular genetic analyses, quantitative mass spectrometry of laser micro‐dissected polyglucosan bodies and muscle homogenate, immunohistochemistry and western blot analyses in muscle tissue from a 45‐year‐old patient with proximal muscle weakness from late teenage years due to polyglucosan storage myopathy.ResultsThe muscle tissue exhibited a complete absence of glycogenin‐1 due to a novel homozygous deep intronic variant in GYG1 (c.7+992T>G), introducing a pseudo‐exon causing frameshift and a premature stop codon. Accumulated proteins in the polyglucosan bodies constituted components of glycogen metabolism, protein quality control pathways and desmin. Muscle fibres containing polyglucosan bodies frequently exhibited depletion of normal glycogen.ConclusionsThe absence of glycogenin‐1, a protein important for glycogen synthesis initiation, causes storage of polyglucosan that displays accumulation of several proteins, including those essential for glycogen synthesis, sequestosome 1/p62 and desmin, mirroring findings in RBCK1 deficiency. These results suggest shared pathogenic pathways across different diseases exhibiting polyglucosan storage. Such insights have implications for therapy in these rare yet devastating and presently untreatable disorders.
Activation of PKC affects the ventricular restitution properties and arrhythmogenesis through L‐type Ca+ current
Authors: Zhang Feng , Fan Jianing , Lei Fuhua , Liu Tao , Lin Dawei , Qin Mu , Cheng Wenbo 
Published: June 2024

AbstractObjectiveTo investigate the role of protein kinase C (PKC) in action potential duration (APD) restitution and ventricular tachyarrhythmias (VAs).Methods and resultsRabbits hearts were isolated and prepared for Langendorff perfusion technique. The stimuli–extra‐stimulus (S1–S2) method and dynamic S1 pacing protocol were performed to construct APD restitution and to induce APD alternans or VA, respectively, at 10 sites throughout the ventricular chamber. Administration of phorbol‐12‐myristate‐13‐acetate (PMA) (100 nM) (n = 15) greatly steepened the restitution curves (Smax > 1) (p < .01) at each site compared to the control group (n = 15). Furthermore, treatment with PMA also induced larger spatial dispersions of Smax (p < .05) and decreased the thresholds of the VA and APD alternans (p < .01). However, perfused with the PKC inhibitor, bisindolylmaleimide (BIM) (500 nM) (n = 10), reversibly flattened the APD restitution curves at each site (Smax < 1), decreased the spatial dispersions of Smax, and increased the thresholds of APD alternans and VA. According to the results of patch‐clamp, peak amplitude of L‐type Ca2+ current was significantly increased by addition of PMA compared with control (CTL) group (p < .05). Antagonize this current with verapamil (n = 10) can fully inhibited the PMA induced increasing of Smax and inducibility of VA and alternans.ConclusionPKC activation increased the dispersion of APD restitution and thus led to occurrence of VA, which possibly related to the increased Ca2+ influx.
Point‐of‐care STI testing in rural settings: A feasibility project
Authors: Townsend Haley , Hobbs Jill , Kaylor Sara , Cody Shameka , Callihan Mike 
Published: June 2024

AbstractSexually transmitted infections (STIs) continue to be a health concern in the United States, particularly in rural settings. Point‐of‐care (POC) STI testing is useful in overcoming access barriers in these settings. The purpose of this project was to implement POC STI testing to increase access to care in rural settings and test the feasibility of this model. Six mobile health clinics were implemented by a nurse‐led team in three rural communities in the Blackbelt region of Alabama utilizing the Binx IO® to test for chlamydia and gonorrhea. Mobile clinic volunteers participated in focus groups to determine provider satisfaction with clinic workflow. The mobile health clinics were a feasible way to increase access to STI testing in rural settings with reported provider and client satisfaction in work flow.
New insights into cancer pathology learned from the dynamics of cancer‐associated fibroblasts
Authors: Ishii Genichiro 
Published: June 2024

AbstractPaget's “Seed and Soil” theory, proposed in 1889, emphasizes the importance of the microenvironment where cancer cells grow in metastatic sites. Over a century later, this concept remains a cornerstone in comprehending cancer biology and devising treatment strategies. The “Seed and Soil” theory, which initially explained how cancer spreads to distant organs, now also applies to the tumor microenvironment (TME) within primary tumors. This theory emphasizes the critical interaction between cancer cells (“seeds”) and their surrounding environment (“soil”) and how this interaction affects both tumor progression within the primary site and at metastatic sites. An important point to note is that the characteristics of the TME are not static but dynamic, undergoing substantial changes during tumor progression and after treatment with therapeutic drugs. Cancer‐associated fibroblasts (CAFs), recognized as the principal noncancerous cellular component within the TME, play multifaceted roles in tumor progression including promoting angiogenesis, remodeling the extracellular matrix, and regulating immune responses. In this comprehensive review, we focus on the findings regarding how the dynamics of CAFs contribute to cancer progression and drug sensitivity. Understanding the dynamics of CAFs could provide new insights into cancer pathology and lead to important advancements in cancer research and treatment.
Genes putatively involved in Phaseolus vulgaris resistance against Xanthomonas phaseoli pv. phaseoli revealed by a genome‐wide association study
Authors: Alves Francisco Henrique Nunes da Silva , Cruz Giovanni Carlos da Silva , Coelho Luis Alexandre Peixoto , Sousa Adryelle Anchieta , Carneiro José Eustáquio de Souza , Badel Jorge Luis 
Published: June 2024

AbstractCarioca and black bean groups play a significant role in Latin American common bean (Phaseolus vulgaris) consumption, serving as excellent sources of nutrients. One of the most significant threats for common bean production is common bacterial blight (CBB), a disease caused by the gram‐negative bacteria Xanthomonas phaseoli pv. phaseoli (Xpp) and Xanthomonas citri pv. fuscans (Xcf). An effective method for managing this disease is the use of plant genetic resistance. However, it is not known whether the same or overlapping sets of genes are associated with resistance to both pathogens. This study aimed to identify a repertoire of genes potentially associated with resistance to Xpp and compare it with that previously reported for Xcf. Eighty carioca and black common bean cultivars that had previously been challenged with Xcf were inoculated with a strain of Xpp, and a genome‐wide association study (GWAS) using single‐nucleotide polymorphisms (SNPs) and disease severity data was conducted. Three carioca and three black bean cultivars exhibited high levels of resistance to Xpp. Five SNPs with the highest −log10(p) values unveiled genes coding for diverse putative biochemical functions, including a light‐regulated Lir1 protein, a proton‐dependent oligopeptide transporter family protein, a metallo‐dependent phosphatase‐like protein, a WPP domain‐interacting tail‐anchored protein 2 and a pectin lyase‐like superfamily protein. No overlap between this repertoire of genes with that reported for Xcf was found. These findings provide important insights into genes potentially involved in the P. vulgaris resistance to Xpp and the complexity of the resistance response to CBB.
Endodermal suberin restricts root leakage of cesium: a suitable tracer for potassium
Authors: Vestenaa Morten Winther , Husted Søren , Minutello Francesco , Persson Daniel Pergament 
Published: June 2024

AbstractAn urgent challenge within crop production is to maintain productivity in a world plagued by climate change and its associated plant stresses, such as heat, drought and salinity. A key factor in this endeavor is to understand the dynamics of root suberization, and its role in plant‐water relations and nutrient transport. This study focuses on the hypothesis that endodermal suberin, acts as a physical barrier preventing radial potassium (K) movement out of the vascular tissues during translocation. Previous attempts to experimentally support this idea have produced inconsistent results. We developed a Laser Ablation‐Inductively Coupled Plasma‐Mass Spectrometry (LA‐ICP‐MS) method, allowing us to visualize the distribution of mineral elements and track K movement. Cesium (Cs), dosed in optimized concentrations, was found to be an ideal tracer for K, due to its low background and similar chemical/biological properties. In suberin mutants of Arabidopsis thaliana, we observed a positive correlation between suberin levels and K translocation efficiency, indicating that suberin enhances the plant's ability to retain K within the vascular tissues during translocation from root to shoot. In barley (Hordeum vulgare), fully suberized seminal roots maintained higher K concentrations in the stele compared to younger, less suberized root zones. This suggests that suberization increases with root maturity, enhancing the barrier against K leakage. In nodal roots, suberin was scattered towards the phloem in mature root zones. Despite this incomplete suberization, nodal roots still restrict outward K movement, demonstrating that even partial suberin barriers can significantly reduce K loss. Our findings provide evidence that suberin is a barrier to K leakage during root‐to‐shoot translocation. This understanding is crucial to maintain crop productivity in the face of climate change.
How to explain the beneficial effects of leukocyte‐ and platelet‐rich fibrin
Authors: Blanco Juan , García Angel , Hermida‐Nogueira Lidia , Castro Ana B. 
Published: June 2024

AbstractThe survival of an organism relies on its ability to repair the damage caused by trauma, toxic agents, and inflammation. This process involving cell proliferation and differentiation is driven by several growth factors and is critically dependent on the organization of the extracellular matrix. Since autologous platelet concentrates (APCs) are fibrin matrices in which cells, growth factors, and cytokines are trapped and delivered over time, they are able to influence that response at different levels. The present review thoroughly describes the molecular components present in one of these APCs, leukocyte‐ and platelet‐rich fibrin (L‐PRF), and summarizes the level of evidence regarding the influence of L‐PRF on anti‐inflammatory reactions, analgesia, hemostasis, antimicrobial capacity, and its biological mechanisms on bone/soft tissue regeneration.
A bibliometric analysis and visualization of acupuncture and moxibustion therapy for herpes zoster and postherpetic neuralgia
Authors: Chen Jianhua , Luo Chao , Ju Peijun , Tu Sijing , Shi Si , Wang Zhaoqin , Wu Huangan 
Published: June 2024

AbstractObjectiveTo identify major contributors, current research status, and to forecast research trends and future development prospects on acupuncture and moxibustion therapy for herpes zoster (HZ) and postherpetic neuralgia (PHN).MethodsA systematic search was conducted on the China National Knowledge Infrastructure (CNKI), Weipu, WanFang databases, and the Web of Science Core Collection (WoSCC), PubMed, and Scopus databases. The search strategy included relevant terms for HZ, PHN, acupuncture, and moxibustion. The reference type was limited to articles or reviews, with a publication date from January 1, 2014 to December 31, 2023. Data analysis was performed using CiteSpace software, focusing on author, institution, source, and keyword distributions, and temporal trends.ResultsA total of 1612 publications were identified from both Chinese and English databases. The analysis revealed a rising trend in publication numbers in the English database, with a significant increase observed in 2020. In the Chinese database, publication activity exhibited two peaks in 2019 and 2023. Guohua Lin and Jingchun Zeng were the most prolific authors in the Chinese and English databases, respectively. The Chengdu University of TCM and Zhejiang Chinese Medicine University were the most active institutions. The keyword analysis revealed “herpes zoster” as the most frequent keyword in the Chinese database, while “postherpetic neuralgia,” “acupuncture,” and “management” were prominent in the English database. The study also identified several therapeutic approaches, including fire needle therapy and electroacupuncture, which have shown efficacy in treating HZ and PHN. Animal studies provided insights into the mechanisms of these therapies, suggesting potential modulation of neuroinflammatory markers and intracellular signaling pathways.ConclusionThe bibliometric analysis underscores the growing interest in acupuncture and moxibustion therapy for HZ and PHN. It highlights the contributions of key authors and institutions while pinpointing potential areas for future research. The study advocates for the necessity of large‐scale, multi‐center clinical trials and further basic mechanical research to optimize these therapies. Moreover, it also emphasizes the importance of international collaboration to strengthen the evidence base and expand the global impact of this traditional treatment modality.
Computational assessment of the aesthetic quality of map colors
Authors: Wu Mingguang , Sun Yanjie , Xia Xianqin 
Published: June 2024

AbstractCrafting beautiful map colors is challenging for not only experts but also novices, suggesting the need for an aesthetic quality assessment of map colors for the effective design of maps and visualizations. To fill this gap, we present a computational method to assess the aesthetic quality of map colors. First, we couple the idea of computational aesthetics with map aesthetic principles and identify four and two basic types of aesthetic features in terms of order and complexity, respectively. Then, we collect 2000 map samples and derive 149 aesthetic metrics by instantiating the above aesthetic features while considering the spatial weights and figure–ground organization of the map samples. We also recruit participants (N = 438) to rate the aesthetic quality of the map colors. Finally, we train an aesthetic predictor by fitting those aesthetic metrics with user ratings. The experimental results show that the proposed method can assess aesthetic quality of map colors with high accuracy (R2 = 0.73 on the training set and R2 = 0.65 on the validation set). We also explore the dominant aesthetic metrics that positively and negatively influence aesthetic appreciation, as well as those metrics that have no significant influence. This work offers a portable and flexible aesthetic quality assessment approach for map colors and can be further improved by considering complex symbols, spatial structures, and color–semantic and color–emotion associations.
An overview of systematic reviews of clinical studies of platelet‐rich plasma for venous ulcers
Authors: Shi Tianbo , Hu Shouci , Hui Jing , Ji Yue , Zhang Yalan 
Published: June 2024

AbstractTo evaluate the clinical evidence of platelet‐rich plasma (PRP) in the treatment of venous ulcers (VUs). Electronic searches were conducted through the Cochrane Library, Web of Science, Embase and PubMed. AMSTAR‐2 was used to assess the methodological quality. The quality of evidence was assessed using the GRADE system. According to AMSTAR‐2, the methodological quality of the included reviews was generally inadequate owing to the limitations of entries 2, 4 and 7. Due to bias risk and imprecision, the evidence quality of the outcome measures was inadequate. In conclusion, PRP may have a therapeutic effect on VUs. However, this conclusion must be treated with caution due to methodological flaws of the included systematic reviews and meta‐analyses.
Reviewer Acknowledgements for Journal of Education and Training Studies, Vol. 12, No. 3
Authors: Smith Robert 
Published: June 2024

Journal of Education and Training Studies (JETS) would like to acknowledge the following reviewers for their assistance with peer review of manuscripts for this issue. Many authors, regardless of whether JETS publishes their work, appreciate the helpful feedback provided by the reviewers. Their comments and suggestions were of great help to the authors in improving the quality of their papers. Each of the reviewers listed below returned at least one review for this issue.Reviewers for Volume 12, Number 3 Ashraf Elsafty, Eslsca University, EgyptChris Mutseekwa, Bindura University of Science Education, ZimbabweEhab Gouda Tolba, Mansoura University, EgyptFathia Lahwal, Elmergib University, LibyaGianpiero Greco, University of Study of Bari, ItalyJacinthe Rihan, Ain Shams University, EgyptJoel Maligaya Cuadra, NU Laguna, PhilippinesJohn Mark Asio, Gordon College, PhilippinesJonathan Chitiyo, University of Pittsburgh Bradford, USAJuan Marcelo Gomez, Seneca Polytechnic, CanadaJulio Cesar André, Center for Studies and Development of Health Education-CEDES, BrazilLinda J. Rappel, Yorkville University/University of Calgary, CanadaMaria Pavlis Korres, Hellenic Open University, GreeceNiveen M. Zayed, MENA College of Management, JordanRichard H. Martin, Mercer University, USARichard Penny, University of Washington Bothell, USASabrine Chbichib, The Higher Institute of Computing and Multimedia Gabes Tunisia, TunisiaSenem Seda Şahenk Erkan, Marmara University, TurkeyStamatis Papadakis, University of Crete, Greece Robert SmithEditorial AssistantOn behalf of, The Editorial Board of Journal of Education and Training StudiesRedfame Publishing9450 SW Gemini Dr. #99416Beaverton, OR 97008, USAE-mail: jets@redfame.comURL: http://jets.redfame.com