bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytosolic acidification and oxidation are the toxic mechanisms of SO2 in Arabidopsis guard cells
Authors: Mozhgani Mahdi , Ooi Lia , Espagne Christelle , Filleur Sophie , Mori Izumi C 
Published: July 2024

ABSTRACT
SO2/H2SO3 can damage plants. However, its toxic mechanism has still been controversial. Two models have been proposed, cytosolic acidification model and cellular oxidation model. Here, we assessed the toxic mechanism of H2SO3 in three cell types of Arabidopsis thaliana, mesophyll cells, guard cells (GCs), and petal cells. The sensitivity of GCs of Chloride channel a (CLCa)-knockout mutants to H2SO3 was significantly lower than those of wildtype plants. Expression of other CLC genes in mesophyll cells and petal cells were different from GCs. Treatment with antioxidant, disodium 4,5-dihydroxy-1,3-benzenedisulfonate (tiron), increased the median lethal concentration (LC50) of H2SO3 in GCs indicating the involvement of cellular oxidation, while the effect was negligible in mesophyll cells and petal cells. These results indicate that there are two toxic mechanisms of SO2 to Arabidopsis cells: cytosolic acidification and cellular oxidation, and the toxic mechanism may vary among cell types.
Mining drug–target interactions from biomedical literature using chemical and gene descriptions-based ensemble transformer model
Authors: Aldahdooh Jehad , Tanoli Ziaurrehman , Tang Jing 
Published: July 2024

Abstract

Motivation
Drug–target interactions (DTIs) play a pivotal role in drug discovery, as it aims to identify potential drug targets and elucidate their mechanism of action. In recent years, the application of natural language processing (NLP), particularly when combined with pre-trained language models, has gained considerable momentum in the biomedical domain, with the potential to mine vast amounts of texts to facilitate the efficient extraction of DTIs from the literature.

Results
In this article, we approach the task of DTIs as an entity-relationship extraction problem, utilizing different pre-trained transformer language models, such as BERT, to extract DTIs. Our results indicate that an ensemble approach, by combining gene descriptions from the Entrez Gene database with chemical descriptions from the Comparative Toxicogenomics Database (CTD), is critical for achieving optimal performance. The proposed model achieves an F1 score of 80.6 on the hidden DrugProt test set, which is the top-ranked performance among all the submitted models in the official evaluation. Furthermore, we conduct a comparative analysis to evaluate the effectiveness of various gene textual descriptions sourced from Entrez Gene and UniProt databases to gain insights into their impact on the performance. Our findings highlight the potential of NLP-based text mining using gene and chemical descriptions to improve drug–target extraction tasks.

Availability and implementation
Datasets utilized in this study are accessible at https://dtis.drugtargetcommons.org/.
CCDC189 depletion leads to oligo-astheno-teratozoospermia and male infertility in mice
Authors: Zhou Huiping , Yang Fan , Li Guanghua , Yuan Lu , Ge Tingting , Niu Changmin , Zheng Ying 
Published: July 2024

Abstract
In male reproductive system, proteins containing the coiled-coil domain (CCDC) are predominantly expressed in specific regions including the testis, epididymis, seminal vesicle, and prostate. They play a vital role in centriole formation, sperm motility and flagellar development in male gametes. Despite being highly expressed in the testis, the exact physiological function of the coiled-coil domain-containing 189 (Ccdc189) gene remain largely unclear. Our research provides a comprehensive and detailed investigation into the localization of CCDC189 protein within the testis seminiferous tubules. CCDC189 specifically expressed in spermatocytes, round spermatids, and elongating spermatids in mouse testis. The deletion of Ccdc189 in mouse leads to male infertility, characterized by significantly reduced sperm counts and motility. Abnormally shaped spermatozoa with irregular tails, exhibiting shortened and twisted morphology, were observed in the seminiferous tubules. Electron microscopy revealed disordered and missing peripheral microtubule doublets (MTD) and outer dense fibers (ODF) in the sperm flagella, accompanied by a consistent absence of central pairs (CP). The knockout of Ccdc189 resulted in oligo-astheno-teratozoospermia, which is characterized by low sperm count and reduced sperm motility and abnormal morphology. Furthermore, we identified poly(A)-binding protein cytoplasmic 1 (PABPC1) and PABPC2 as interacting proteins with CCDC189. These proteins belong to the PABP family and are involved in regulating mRNA translational activity in spermatogenic cells by specifically binding to poly(A) tails at the 3′ ends of mRNAs.
Generative Artificial Intelligence: Enhancing Patient Education in Cardiovascular Imaging
Authors: Marey Ahmed , Saad Abdelrahman M , Killeen Benjamin D , Gomez Catalina , Tregubova Mariia , Unberath Mathias , Umair Muhammad 
Published: July 2024

AbstractCardiovascular disease (CVD) is a major cause of mortality worldwide, especially in resource-limited countries with limited access to healthcare resources. Early detection and accurate imaging are vital for managing CVD, emphasizing the significance of patient education. Generative artificial intelligence (AI), including algorithms to synthesize text, speech, images, and combinations thereof given a specific scenario or prompt, offers promising solutions for enhancing patient education. By combining vision and language models, generative AI enables personalized multimedia content generation through natural language interactions, benefiting patient education in cardiovascular imaging. Simulations, chat-based interactions, and voice-based interfaces can enhance accessibility, especially in resource-limited settings. Despite its potential benefits, implementing generative AI in resource-limited countries faces challenges like data quality, infrastructure limitations, and ethical considerations. Addressing these issues is crucial for successful adoption. Ethical challenges related to data privacy and accuracy must also be overcome to ensure better patient understanding, treatment adherence, and improved healthcare outcomes. Continued research, innovation, and collaboration in generative AI have the potential to revolutionize patient education. This can empower patients to make informed decisions about their cardiovascular health, ultimately improving healthcare outcomes in resource-limited settings.
Systematic assessment of serum i-tRF-AsnGTT in gastric cancer: a potential clinical biomarker
Authors: Jiang Xiaodan , Li Xun , Li Yang , Zhang Yu , Gu Xinliang , Zong Wei , Shen Xianjuan , Ju Shaoqing 
Published: July 2024

Abstract
Since gastric cancer (GC) shows no apparent signs in its early stages, most patients are diagnosed later with a poor prognosis. We therefore seek more sensitive and specific GC biomarkers. Small RNAs formed from tRNAs represent a novel class of non-coding RNAs that are highly abundant in bodily fluids and essential to biological metabolism. This study explores the potential of i-tRF-AsnGTT in gastric cancer diagnostics. To begin with, we sequenced i-tRF-AsnGTT using high-throughput methods. i-tRF-AsnGTT expression levels in GC were determined using real-time fluorescence polymerase chain reaction. Agarose gel electrophoresis, Sanger sequencing, and repeated freezing and thawing were performed to verify molecular properties. A correlation was found between clinical and pathological parameters and i-tRF-AsnGTT expression levels through the χ2 test, and receiver operating characteristic was used to analyze its diagnostic value in GC. In serum, i-tRF-AsnGTT has a low and stable expression level. It can differentiate between patients with gastric cancer and gastritis and healthy donors with better diagnostic efficacy. In combination with clinicopathological parameters, i-tRF-AsnGTT correlates with tumor differentiation; infiltration depth of tumors; tumor, node, metastasis stage; lymph node metastases; and neural/vascular invasion. Serum i-tRF-AsnGTT expression is low in GC patients. Serum from postoperative patients shows increased i-tRF-AsnGTT expression levels. Potentially, this could be used as a biomarker to help diagnose gastric cancer and monitor its prognosis.
Multiomic profiling of new-onset kidney function decline: insights from the STANISLAS study cohort with a 20-year follow-up
Authors: Dupont Vincent , Xhaard Constance , Behm-Ansmant Isabelle , Bresso Emmanuel , Thuillier Quentin , Branlant Christiane , Lopez-Sublet Marilucy , Deleuze Jean-François , Zannad Faiez , Girerd Nicolas , Rossignol Patrick 
Published: July 2024

ABSTRACT

Background
Identifying the biomarkers associated with new-onset glomerular filtration rate (GFR) decrease in an initially healthy population could offer a better understanding of kidney function decline and help improving patient management.

Methods
Here we described the proteomic and transcriptomic footprints associated with new-onset kidney function decline in an initially healthy and well-characterized population with a 20-year follow-up. This study was based on 1087 individuals from the familial longitudinal Suivi Temporaire Annuel Non-Invasif de la Santé des Lorrains Assurés Sociaux (STANISLAS) cohort who attended both visit 1 (from 1993 to 1995) and visit 4 (from 2011 to 2016). New-onset kidney function decline was approached both in quantitative (GFR slope for each individual) and qualitative (defined as a decrease in GFR of &gt;15 ml/min/1.7 m2) ways. We analysed associations of 445 proteins measured both at visit 1 and visit 4 using Olink Proseek® panels and 119 765 genes expressions measured at visit 4 with GFR decline. Associations were assessed using multivariable models. The Bonferroni correction was applied.

Results
We found several proteins (including PLC, placental growth factor (PGF), members of the tumour necrosis factor receptor superfamily), genes (including CCL18, SESN3), and a newly discovered miRNA—mRNA pair (MIR1205–DNAJC6) to be independently associated with new-onset kidney function decline. Complex network analysis highlighted both extracellular matrix and cardiovascular remodelling (since visit 1) as well as inflammation (at visit 4) as key features of early GFR decrease.

Conclusions
These findings lay the foundation to further assess whether the proteins and genes herein identified may represent potential biomarkers or therapeutic targets to prevent renal function impairment.
Human Tales on the Pathless Sea? Imperial Subjectivities and Exploration Ship Tracks in European Maritime Mapping, c.1500–c.1800
Authors: Caputo Sara 
Published: July 2024

Abstract
During the Renaissance, Europeans began to mark sea journeys on maps and charts as lines or dotted lines—‘ship tracks’. This new graphical depiction of voyages, which flanked and often replaced verbal itinerary descriptions, ultimately lies behind present-day route-marking devices, but it has not been much investigated by historians. Tracing its development between the sixteenth and the early nineteenth centuries, through cartographic sources, hydrographical manuals and voyage accounts, this article argues that ship tracks constituted a fundamental turning point in European understandings of human movement and influence in the world. In particular, the tracks of late eighteenth-century imperial explorers, built through novel accuracy claims, established a unique cognitive and ontological link between representation and reality and between human agency and the natural world, and explicitly embedded the perspectival subjectivity of storytelling into the alleged objectivity of ‘scientific’ geometrisations of the globe. As such, tracks are useful tools with which to understand and deconstruct the roots of ‘modernity/coloniality’. They also reveal the extent to which maritime history holds important keys to the past: as a representational and directional device, the modern journey track owes much of its ancestry to confrontation with the liquid and supposedly ‘pathless’ sea.
Preventing Child Maltreatment: Beneficial Side Effects of Public Childcare
Authors: Sandner Malte , Thomsen Stephan L , González Libertad 
Published: July 2024

Abstract
We investigate the impact of public childcare provision on the incidence of severe child maltreatment. For identification, we exploit a reform that expanded early childcare in Germany, generating large temporal and spatial variation in childcare coverage at the county level. Using high-quality administrative data covering all reported cases of child maltreatment in Germany by county and year, we find that an increase in childcare slots by one percentage point in a county reduced child maltreatment cases leading to out-of-home placement by about 1%. Our results suggest that the provision of universal public childcare may be more cost effective than previously thought.
Clinical and echographic parameters as risk factors for atrial fibrilation in patients with hypertophic cardiomyopathy
Authors: Panagiotou C , Marinopoulos E , Linardos S 
Published: July 2024

Abstract

Introduction
Hypertrophic cardiomyopathy is the most prevalent inherited cardiomyopathy characterized by left ventricular hypertrophy and manifests with atrial fibrillation in 25% of patients, correlating with heightened morbidity and mortality rates.

Purpose
To investigate the clinical and echocardiographic parameters associated with the occurrence and progression of atrial fibrillation in patients diagnosed with hypertrophic cardiomyopathy.

Material and Methods
The study sample consisted of 72 patients with hypertrophic cardiomyopathy attending the Hereditary Heart Disease Unit at a tertiary cardiac surgery center. Thorough examination of patients' medical records was conducted.

Results
Myocardial fibrosis was observed in 68.1% of the patients. Analysis of the data revealed that atrial fibrillation (AF) episodes were more prevalent in older individuals (p=0.035) with a lower ejection fraction (p=0.003). Additionally, a larger left atrial diameter of 49.8mm (p&lt;0.001), functional stage according to NYHA III (p=0.015), a history of syncope/pre-syncope events (p=0.018), patients with an implanted pacemaker/defibrillator (p=0.003), and those with known coronary artery disease factors (p=0.032), particularly dyslipidemia (p=0.049) and diabetes mellitus (p=0.027), were associated with higher AF incidence.

Conclusions
. This study underscores the importance of recognizing clinical and echocardiographic factors that contribute to atrial fibrillation risk in hypertrophic cardiomyopathy, enabling early identification of high-risk individuals and implementation of preventive strategies to reduce the frequency of atrial fibrillation episodes and associated complications.
EASI monitoring in ventricular tachycardia, an alternative to conventional electrocardiography?
Authors: Pina Astete L , Buelga Suarez M L , Munoz Gomez M I , Rodriguez Sanchez P , Tur Sainz A , Penaranda Romero N , Garcia Montalvo J , Rebolleda Sanchez A , Alonso Salinas G L 
Published: July 2024

Abstract

Background
12-lead electrocardiogram (ECG) is the gold standard for detecting ventricular arrhythmias. In cardiac critical care units (UCC), monitoring systems are used to detect transient events such as arrhythmias or ST-segment acute changes. The discomfort caused in the patient by the need for 10 electrodes to obtain the 12 leads continuously means that complete monitoring is not routine.
The EASI monitoring system, based on vector electrocardiography, is an alternative that uses only 5 electrodes (4 thoracic and 1 ground) and theoretically allows obtaining a complete standard ECG.

Objectives
The main endpoint of this study was to evaluate the diagnostic accuracy of the EASI monitoring system in patients with ventricular tachycardia (VT), compared with conventional ECG.

Methods
Seven cases of patients admitted to the CCU due to episodes of VT are presented. The patients were monitored with the EASI system upon arrival, and the conventional ECG and the one derived from the EASI system were taken simultaneously for VT events for comparison.
The 4 electrodes of the EASI system were placed, as recommended, on the sternal manubrium (S), lower sternum at the level of the 5th intercostal space (E), at the level of the 5th intercostal space, and on the right and left midclavicular lines (I and A).

Results
Analysing case by case, the axis of the tachycardia was similar in 5 patients, being able to assess the origin of the tachycardia in them. In 2 patients it was different. These cases of patients that differ, one episode has its origin in the right ventricular outflow tract (endocardial) and the other in the area of the left ventricular summit (epicardial). Two patients with different tachycardias and no cardiac characteristics in common. Figure 1.

Conclusions
EASI monitoring is a feasible alternative for monitoring patients in the CCU, which could give us reliable information (compared with standard ECG) with limitations in some cases in which the cardiac axis differs. For patients admitted for arrhythmic events, a better characterization of the differences would be interesting in order to optimize its use.
Quality of life in patients with heart failure and implantable cardioverter defibrillator. Results from 42 heart failure clinics in norway
Authors: Flagtvedt H , Torstein Hole T H , Tore Wentzel-Larsen T W L , Christer Aase C A A , Tone M Norekvaal T M N 
Published: July 2024

Abstract

Background
Patients with heart failure (HF) have an increased risk of sudden cardiac death, and treatment with an implantable cardioverter defibrillator (ICD) has become standard treatment for selected patient groups. HF is associated with impaired QoL, but there is no consensus regarding the implication of having an ICD. Knowledge regarding QoL in patients with HF and ICD attending follow-up in outpatient HF-clinics is sparse.

Purpose
The aim of this study was to determine QoL in patients with HF and ICD. Further, to compare QoL in patients with HF with and without ICD, using data from the first and follow-up visits in outpatient HF clinics.

Methods
Longitudinal data from the National Norwegian Heart Failure Registry (NNHFR), including 9 274 patients, of which 1 206 with an ICD, were studied. QoL was assessed using the Minnesota Living with Heart Failure Questionnaire (MLHFQ). Data was collected at the first and follow-up visits in outpatient HF clinics approximately six months after optimal treatment was achieved.

Results
Patients with an ICD were younger, more often men, and had higher frequency of coronary artery disease (all p&lt;0.001). At the first visit, there was no significant difference in QoL between patients with and without ICD, with both groups reporting moderate QoL. Both groups had a significant improvement in QoL from the first visit to the follow-up visit (p&lt;0.001). However, patients with ICD reported significantly less improvement in QoL (p&lt;0.001) at follow-up. This disparity was evident regarding both the physical (p&lt;0.001) and the emotional (p&lt;0.001) dimension.

Conclusion
QoL in patients with HF and ICD improves after follow-up in outpatient HF clinics, although not to the same extent as in patients without ICD. Thus, tailored interventions in outpatient HF clinics may be necessary to improve outcomes in this subgroup of patients with both HF and ICD.
The STEP-ONE program (Supportive Training and Essential Preparation for Optimal Nursing Education) - what have we done and what will we do in the STEP-TWO edition?
Authors: Uchmanowicz I , Lisiak M H , Wleklik M , Sawielajc L , Turgonyi E , Ponikowski P , Jankowska E A 
Published: July 2024

Abstract

Introduction
The STEP-ONE program was a significant initiative designed to enhance the skills and knowledge of nurses specializing in heart failure care in Central and Eastern Europe, and the Baltic countries (CEE-BA).

Purpose
Its importance lies in addressing the crucial role of nurses in heart failure management, an area often overlooked in these regions. By training nurses to provide optimal, integrated care, the program aims to improve patient outcomes and reduce hospitalizations. The number of nurses who participated in the program is not specified, but its impact is likely substantial in advancing heart failure care. Continuing the program as the STEP-TWO edition is planned to be updated of nurses and patients materials according to ESC HF 2023 guidelines6, taking into account the profile of the patient with HFrEF, HFmREF, HFimEF, HFpEF and taking into account the implementation of pilot projects: (1a) Assessment of HFpEF risk7 and recommendations for medical staff regarding further diagnostics; (1b) Analysis of recommended therapies according to guidelines; (2) Pilot training Program center Poland centers- CEE-BA; (3) Pilot research programs - 1a and 1/b in 2-3 centers (fig.1). All of the activities undertaken in the program are intended to improve care for heart failure patients and can be seen as a model solution for providing qualified personnel in the European countries.

Conclusions
The STEP ONE educational program was developed with the participation of the medical community, especially nurses caring for HF patients. Educational materials for nurses and patients, as well as an educational program, were prepared and implemented in Poland and the region of CEE-BA. During the second phase, an updated materials with the 2023 guidelines on HF will be prepared. A pilot study will evaluate the impact of educating HF nurses on patient risk assessment of HFPEF and on identifying patients who do not have optimal therapy according to GDMT. Optimization of the HF patient pathway at the pilotage centers of the program and collaboration between HF nurses and cardiologists will be sought. Moreover, the STEP-TWO program will include preparing educational materials focused and dedicated not only on patients but also on caregivers.