bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytomegalovirus-Induced Expression of CD244 after Liver Transplantation Is Associated with CD8+ T Cell Hyporesponsiveness to Alloantigen
Authors: de Mare-Bredemeijer Emmy L. D. , Shi Xiao-lei , Mancham Shanta , van Gent Rogier , van der Heide-Mulder Marieke , de Boer Renate , Heemskerk Mirjam H. M. , de Jonge Jeroen , van der Laan Luc J. W. , Metselaar Herold J. , Kwekkeboom Jaap 
Published: July 2015

Abstract
The chronic presence of viral Ags can induce T cell exhaustion, which is characterized by upregulation of coinhibitory receptors and loss of T cell function. We studied whether a similar phenomenon occurs after liver transplantation (LTx), when there is continuous exposure to alloantigen. Expression of coinhibitory receptors on circulating CD4+ and CD8+ T cells was analyzed longitudinally in 19 patients until 6 mo after LTx and cross-sectionally in 38 patients late (1–12 y) after LTx. Expression of the coinhibitory receptors CD160 and CD244 on circulating CD8+ T cells was already higher 6 mo after LTx compared with pre-LTx, and the elevated expression was sustained late after LTx, with CD244 showing the more prominent increase. The strongest upregulation of CD244 on circulating CD8+ T cells was observed in patients who experienced CMV infection after LTx. CMV infection also was associated with reduced CD8+ T cell proliferation and cytotoxic degranulation in response to alloantigen late after LTx. Purified CD244+CD8+ T cells from LTx patients showed lower proliferative responses to alloantigen, as well as to polyclonal stimulation, than did their CD244− counterparts. In addition, the CD244+CD8+ T cell population contained the majority of CMV peptide–loaded MHC class I tetramer-binding cells. In conclusion, CMV infection after LTx, rather than persistence of alloantigen, induces the accumulation of dysfunctional CD244+CD8+ T cells in the circulation that persist long-term, resulting in reduced frequencies of circulating alloreactive CD8+ T cells. These results suggest that CMV infection restrains CD8+ T cell alloresponses after LTx.
Restriction of Nonpermissive RUNX3 Protein Expression in T Lymphocytes by the Kozak Sequence
Authors: Kim Byungil , Sasaki Yo , Egawa Takeshi 
Published: July 2015

Abstract
The transcription factor Runx3 promotes differentiation of naive CD4+ T cells into type-1 effector T (TH1) cells at the expense of TH2. TH1 cells as well as CD8+ T cells express a subset-specific Runx3 transcript from a distal promoter, which is necessary for high protein expression. However, all T cell subsets, including naive CD4+ T cells and TH2 cells, express a distinct transcript of Runx3 that is derived from a proximal promoter and that produces functional protein in neurons. Therefore, accumulation of RUNX3 protein generated from the proximal transcript needs to be repressed at the posttranscriptional level to preserve CD4+ T cell capability of differentiating into TH2 cells. In this article, we show that expression of RUNX3 protein from the proximal Runx3 transcript is blocked at the level of translational initiation in T cells. A coding sequence for the proximal Runx3 mRNA is preceded by a nonoptimal context sequence for translational initiation, known as the Kozak sequence, and thus generates protein at low efficiencies and with multiple alternative translational initiations. Editing the endogenous initiation context to an “optimal” Kozak sequence in a human T cell line resulted in enhanced translation of a single RUNX3 protein derived from the proximal transcript. Furthermore, RUNX3 protein represses transcription from the proximal promoter in T cells. These results suggest that nonpermissive expression of RUNX3 protein is restricted at the translational level, and that the repression is further enforced by a transcriptional regulation for maintenance of diverse developmental plasticity of T cells for different effector subsets.
The role of master regulators in gene regulatory networks
Authors: Hernández Lemus Enrique , Baca López K. , Lemus R. , García Herrera R. 
Published: July 2015

Gene regulatory networks present a wide variety of dynamical responses to intrinsic and extrinsic perturbations. Arguably, one of the most important of such coordinated responses is the one of amplification cascades, in which activation of a few key-responsive transcription factors (termed master regulators, MRs) lead to a large series of transcriptional activation events. This is so since master regulators are transcription factors controlling the expression of other transcription factor molecules and so on. MRs hold a central position related to transcriptional dynamics and control of gene regulatory networks and are often involved in complex feedback and feedforward loops inducing non-trivial dynamics. Recent studies have pointed out to the myocyte enhancing factor 2C (MEF2C, also known as MADS box transcription enhancer factor 2, polypeptide C) as being one of such master regulators involved in the pathogenesis of primary breast cancer. In this work, we perform an integrative genomic analysis of the transcriptional regulation activity of MEF2C and its target genes to evaluate to what extent are these molecules inducing collective responses leading to gene expression deregulation and carcinogenesis. We also analyzed a number of induced dynamic responses, in particular those associated with transcriptional bursts, and nonlinear cascading to evaluate the influence they may have in malignant phenotypes and cancer.Received: 20 Novembre 2014, Accepted: 24 June 2015; Edited by: C. A. Condat, G. J. Sibona; DOI: http://dx.doi.org/10.4279/PIP.070011Cite as: E Hernández-Lemus, K Baca-López, R Lemus, R García-Herrera, Papers in Physics 7, 070011 (2015)This paper, by E Hernández-Lemus, K Baca-López, R Lemus, R García-Herrera, is licensed under the Creative Commons Attribution License 3.0.
Role of energy uncertainties in ergodicity breaking induced by competing interactions and disorder. A dynamical assessment through the Loschmidt echo
Authors: Zangara Pablo R. , Levstein P. R. , Pastawski H. M. 
Published: July 2015

A local excitation in a quantum many-particle system evolves deterministically. A time-reversal procedure, involving the invertion of the signs of every energy and interaction, should produce an excitation revival: the Loschmidt echo (LE). If somewhat imperfect, only a fraction of the excitation will refocus. We use such a procedure to show how non-inverted weak disorder and interactions, when assisted by the natural reversible dynamics, fully degrade the LE. These perturbations enhance diffusion and evenly distribute the excitation throughout the system. Such a dynamical paradigm, called ergodicity, breaks down when either the disorder or the interactions are too strong. These extreme regimes give rise to the well known Anderson localization and Mott insulating phases, where quantum diffusion becomes restricted. Accordingly, regardless of the kinetic energy terms, the excitation remains mainly localized and out-of-equilibrium, and the system behaves non-ergodically. The LE constitutes a fair dynamical witness for the whole phase diagram since it evidences a surprising topography in which ergodic and non-ergodic phases interpenetrate each other. Furthermore, we provide an estimation for the critical lines separating the ergodic and non-ergodic phases around the Mott and Anderson transitions. The energy uncertainties introduced by disorder and interaction shift these thresholds towards stronger perturbations. Remarkably, the estimations of the critical lines are in good agreement with the phase diagram derived from the LE dynamics.Received: 20 Novembre 2014, Accepted: 29 June 2015; Edited by: C. A. Condat, G. J. Sibona; Reviewed by: A. De Luca, Laboratoire de Physique Theorique, ENS & Institut Philippe Meyer, Paris, France; DOI: http://dx.doi.org/10.4279/PIP.070012Cite as: P R Zangara, P R Levstein, H M Pastawski, Papers in Physics 7, 070012 (2015)This paper, by P R Zangara, P R Levstein, H M Pastawski, is licensed under the Creative Commons Attribution License 3.0.
Influence of Chemical Degradation and Toothbrushing on Surface of Composites
Authors: Borges Alessandra Buhler , Pucci Cesar Rogério , Torres Carlos Rocha Gomes , Silva Tânia Mara Da , Sales Ana Luísa Leme Simões 
Published: July 2015

ABSTRACT

Objective
The aim of this study was to evaluate the effect of chemical degradation media associated with brushing on surface roughness (Ra) and Knoop microhardness (KHN) analyses of different composites.

Materials and methods
Eighty specimens were prepared for each composite: GrandioSO (Voco), Amaris (Voco), Filtek Supreme (3M ESPE), Filtek LS (3M ESPE). The specimens were divided into four groups according to the immersion in chemical degradation media for 7 days: artificial saliva (control), heptane, 0.02 M citric acid, 70% ethanol. The surface of specimens was submitted to 10950 brushing cycles (200 gm load) in an automatic toothbrushing machine with abrasive slurry. Surface roughness and microhardness measurements were obtained at baseline, after immersion in chemical degradation media and after brushing. Data were submitted to three-way repeated measures ANOVA and Tukey's test (p < 0.05).

Results
The KHN means for composites were: Grandio (153.5 ± 35.9)a, Filtek Supreme (87.0 ± 24.9)b, Amaris (64.5 ± 24.5)c, LS (69.0 ± 15.3)c; for storage media: artificial saliva (97.3 ± 46.7)a, ethanol (93.3 ± 49.9)a, citric acid (95.8 ± 42.1)a, heptane (87.6 ± 36.7)b; and after treatments: after chemical degradation (104.8 ± 49.7)a, baseline (93.8 ± 42.5)b, after brushing (81.9 ± 36.5)c. The Ra results for composites were: LS (0.15 ± 0.25)a, GrandioSO (0.19 ± 0.24)ab, Filtek Supreme (0.20 ± 0.22)ab, Amaris (0.23 ± 0.37)b; for storage media: artificial saliva (0.18 ± 0.31)a, heptane (0.18 ± 0.25)a, ethanol (0.20 ± 0.26)a, citric acid (0.21 ± 0.28)a, and after treatments: brushing (0.11 ± 0.14)a, after chemical degradation (0.23 ± 0.32)b, baseline (0.24 ± 0.32)b.

Conclusion
Brushing after chemical degradation reduced surface roughness values. In general, chemical degradation did not affect composites roughness, but microhardness was significantly reduced. Heptane produced the biggest reduction in composites microhardness.

Clinical relevance
The food-simulating solutions and brushing simulating alter the composites properties, and these alterations are material-dependent.

How to cite this article
Torres CRG, Da Silva TM, Sales ALLS, Pucci CR, Borges AB. Influence of Chemical Degradation and Toothbrushing on Surface of Composites. World J Dent 2015;6(2):65-70.
Clinical Update on Nickel-Titanium Rotary Instruments
Authors: Pawar Suraj , Keswani Ekta , Jain Priyanka , Nayan Swapna 
Published: July 2015

ABSTRACT
In the last decade, nickel-titanium (NiTi) rotary canal instruments have proved to be valuable for root canal therapy because of their exceptional ability to shape root canals with potentially fewer procedural complications. The purpose of this clinical update article is to bring clinicians up to date with the current and popular endodontic rotary NiTi instrument systems.

How to cite this article
Jain P, Nayan S, Keswani E, Pawar S. Clinical Update on Nickel-Titanium Rotary Instruments. World J Dent 2015;6(2):97-103.
Microgravity might affect Peri-implant Mucosal Epithelial cells during space Flight
Authors: Mukai Chisato , Nakamoto Tetsuji , Kondo Yusuke , Masaki Chihiro , Tamura Akiko , seo Yoshinori , Mukaibo Taro , Hosokawa Ryuji 
Published: July 2015

ABSTRACT

Purpose
The effect of microgravity on gingival epithelial cells (GE1) is unknown; thus, we analyzed cell proliferation as well as the gene expression patterns in GE1 cells cultured under simulated microgravity.

Materials and methods
Gingival epithelial cells were seeded and cultured at 10-3 G in a three-dimensional clinostat to simulate microgravity (group CL) or in normal gravity (group C) for 10 days. Cell proliferation was analyzed by counting the numbers of cells. Real-time polymerase chain reaction was performed to amplify the krt 5, krt 13 and involucrin genes. Additionally, total protein was immunoblotted with anti-krt 13 antibody. Statistical analysis (n = 9, three groups repeated three times) was performed (ANOVA, Tukey's test, p < 0.05).

Results
Cell proliferation was significantly upregulated under microgravity based on the average number of cells. Cell proliferation and differentiation marker expression was significantly increased after culture under simulated microgravity. Western blotting showed intense krt 13 staining under simulated microgravity. The simulated microgravity environment had an accelerating effect on GE1 proliferation and differentiation.

Conclusion
These findings suggest that GE1 cells would be affected by the microgravity environment during space flight. Moreover, these findings also suggest that we could promote regeneration of gingival cells using of a simulated microgravity environment.

How to cite this article
Tamura A, Masaki C, Seo Y, Mukai C, Mukaibo T, Kondo Y, Nakamoto T, Hosokawa R. Microgravity might affect Peri-implant Mucosal Epithelial Cells during Space Flight. Int J Prosthodont Restor Dent 2015;5(1):10-16.
A Simplified Technique for Fabrication and Characterization of a Custom Ocular Prosthesis using Light-cured Stains
Authors: Chitre Vidya , Daswani Sohil Rajkumar , Aras Meena Ajay , Mysore Ashwin 
Published: July 2015

ABSTRACT
An ocular prosthesis which gives attention to the accurate duplication of natural color, contour, size and ocular orientation will provide realism and symmetry for patients who need it. This article describes a simplified technique of converting a stock eye shell into a well-fitting custom ocular prosthesis that requires minimal clinical procedures and simplified laboratory procedures. The color matching of the sclera of the prosthesis was performed using light-cured resin-based stains that are simple to use and give a more predictable and lifelike appearance when compared to the conventional acrylic ‘paint on’ technique.

How to cite this article
Daswani SR, Aras MA, Chitre V, Mysore A. A Simplified Technique for Fabrication and Characterization of a Custom Ocular Prosthesis using Light- cured Stains. Int J Prosthodont Restor Dent 2015;5(1):17-20.
The Clinical Application Of Platelet Concentrates: A Systematic Meta-analysis
Authors: Gupta Pankaj , Gada Sneha Ketan 
Published: July 2015

ABSTRACT

Background
Autologous plasma rich in platelets is derived blood product whose uses in dentistry dates back to the 1990s and it has been gaining popularity ever since.

Aim
A systematic review of the available literature to determine the efficacy, safety and success of platelet concentrates in physiological systems as well as describing its vigilant use.

Study design and methods
A Medline search with keywords ‘platelet rich plasma dental’ further filtered using Boolean opera- tors (AND, OR, NOT) and combination of specific keywords as follows: ‘platelet rich fibrin’, ‘platelet rich plasma regenerative’, ‘platelet rich plasma periodontal’, ‘platelet rich plasma extraction’ with a custom range of 10 years was performed, which yielded 88 results out of which 32 were selected based on the inclusion criteria.

Results
Significantly, successful outcomes have been wit- nessed in the field of osteoregeneration though it has been evidenced that adequate platelet rich plasma (PrP) additives are essential to bear a therapeutic potential. Favoring blood cell adhesion on the root surface optimizing periodontal healing, PrP has also shown positive effect on gingival repair; though controversial facts have also been reported. In the scope of implant dentistry, PrP has also reported greater implant-bone contact when used in both gel or liquid form. Emerging as a possible pulp capping agent along with properties of decreased chances root growth, PrP has successfully completed apexo- genesis in cases of pulpotomy.
Research has shown that although clinical results from the comparison of PrP alone vs PrP and resorbable membrane or various graft materials has shown a varied results, additional research in the field is needed.

Conclusion
Platelet rich plasma has shown promising results in the field of regenerative dentistry, with high success rate in implantology, periodontology and oral surgery. Because of conflicting success rates reported in literature further research is warrented.

Clinical implication
Platelet rich fibrin has displayed various features of rapid clinical healing, excellent bone density, less surgical time, less resorption during healing and decreased postoperative pain, as compared to guided bone regeneration procedures. Platelet rich plasma has also shown a myriad of applications ranging from hard tissue regeneration to soft tissue management, stretching across to the field of pediatric dentistry and endodontics.

How to cite this article
Gada SK, Gupta P. the Clinical Application of Platelet Concentrates: A Systematic Meta- analysis. Int J Prosthodont restor Dent 2015;5(1):21-26.
Exit from Exporting: Does Being a Two-way Trader Matter?
Authors: Díaz-Mora Carmen , Córcoles David , Gandoy Rosario 
Published: July 2015

Abstract
The aim of this paper is to investigate whether the probability of ceasing to export is lower for firms that simultaneously import intermediate inputs and export (vertically specialized firms à la Hummels et al., The nature and growth of vertical specialization in world trade, 2001), once other firm characteristics are controlled for. On the basis of the estimation of a random-effects probit model with panel data, the authors find that the superior characteristics of these types of two-way trading firms (in terms of size, productivity, foreign ownership and skilled labor) explain their greater resistance to losing their status as exporters. However, even when these distinctive traits are controlled for, the effect of sourcing inputs from abroad on export exit is significantly different for large and small firms, playing a role in continuing to export only for the latter. Thus, it seems that small firms which are both importers of intermediates and exporters have an added advantage which enables them to confront the uncertainty of foreign markets in better conditions and translates to a lower likelihood that they will stop exporting.
Intraoperative Assessment of Vascular Access
Authors: Saucy Francois , Déglise Sébastien , Holzer Thomas , Salomon Lucie , Engelberger Stephan , Corpataux Jean-Marc 
Published: July 2015

Purpose The intraoperative quality assessment of the arteriovenous fistula for hemodialysis is an essential process to limit early failure due to technical problems or inadequate vascular quality. This step is not clearly defined in the literature with no recommendations. Methods We selected published articles related to the topic of intraoperative quality control of the vascular access for hemodialysis. Results The intraoperative blood flow measurement greater than 120 ml/min in autologous fistula and less than 320 ml/min in arteriovenous graft was described as predictive factors for early failure. Conclusions The blood flow measurement should be performed after the confection of the anastomosis. When blood flow is limited, fistulography is an essential step to assess patency.
MicroRNA-375 Suppresses Extracellular Matrix Degradation and Invadopodial Activity in Head and Neck Squamous Cell Carcinoma
Authors: Jimenez Lizandra , Sharma Ved P. , Condeelis John , Harris Thomas , Ow Thomas J. , Prystowsky Michael B. , Childs Geoffrey , Segall Jeffrey E. 
Published: July 2015

ContextHead and neck squamous cell carcinoma (HNSCC) is a highly invasive cancer with an association with locoregional recurrence and lymph node metastasis. We have previously reported that low microRNA-375 (miR-375) expression levels correlate with poor patient survival, increased locoregional recurrence, and distant metastasis. Increasing miR-375 expression in HNSCC cell lines to levels found in normal cells results in suppressed invasive properties. HNSCC invasion is mediated in part by invadopodia-associated degradation of the extracellular matrix.ObjectiveTo determine whether elevated miR-375 expression in HNSCC cell lines also affects invadopodia formation and activity.DesignFor evaluation of the matrix degradation properties of the HNSCC lines, an invadopodial matrix degradation assay was used. The total protein levels of invadopodia-associated proteins were measured by Western blot analyses. Immunoprecipitation experiments were conducted to evaluate the tyrosine phosphorylation state of cortactin. Human protease arrays were used for the detection of the secreted proteases. Quantitative real time–polymerase chain reaction measurements were used to evaluate the messenger RNA (mRNA) expression of the commonly regulated proteases.ResultsIncreased miR-375 expression in HNSCC cells suppresses extracellular matrix degradation and reduces the number of mature invadopodia. Higher miR-375 expression does not reduce cellular levels of selected invadopodia-associated proteins, nor is tyrosine phosphorylation of cortactin altered. However, HNSCC cells with higher miR-375 expression had significant reductions in the mRNA expression levels and secreted levels of specific proteases.ConclusionsMicroRNA-375 regulates invadopodia maturation and function potentially by suppressing the expression and secretion of proteases.
Cosmopolitisme et nationalisme dans le théâtre d’Edmond Picard : les enjeux identitaires du répertoire théâtral en Belgique francophone à la fin du XIXe siècle
Authors: Vanderpelen-Diagre Cécile 
Published: July 2015

À la fin du XIXe siècle, des tentatives sont lancées pour remettre en question la mainmise de la France sur le répertoire, la direction et les troupes du théâtre francophone belge. Edmond Picard (1836-1924), avocat, mécène, écrivain et homme politique influent, tente de faire pression sur la ville de Bruxelles pour qu’elle impose à son principal théâtre parlé, le Théâtre royal du Parc, un répertoire qui s’ouvre aux auteurs étrangers et belges. Nationaliser le théâtre passe par les pièces de Tolstoï, Ibsen, Maeterlinck et Picard, qui reposent sur des propositions dramaturgiques éloignées du vaudeville français.
Quand H. C. essaie de s’excuser…
Authors: Cixous Hélène 
Published: July 2015

Dans cette lettre écrite sur le vif, Hélène Cixous note quelques traits télégraphiques au sujet de l’ekphrasis, de ce que cette figure signifie pour elle tant dans sa pratique d’écriture que dans sa lecture des oeuvres d’art, que celles-ci soient littéraires ou picturales. L’art « appelle », dit-elle, à l’interprétation (musicale), il a « besoin d’être traduit dans son autre langue ». En art comme en littérature, l’oeuvre est « muette », « flot de paroles autour d’un certain silence ». Cixous évoque ainsi l’essentiel du regard, de l’échange entre « Regarder être regardé » qui se produit en elle : « Quand je regarde je suis absorbée, adoptée par le tableau », « je ne vois que là où ça me ressemble (je ne le découvre qu’après) ».