bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxidative stress markers in brain and gonads of rabbit bucks fed herbal supplements
Authors: Jimoh Olatunji Abubakar 
Published: August 2024

Abstract
Background
Currently, there is an increase in the usage of phytogenic feed additives to help improve animal welfare and productivity, while less emphasis is now placed on metabolic and oxidative stability of neuronal and testicular tissues. This study aims at investigating the effects of Moringa oleifera, Phyllanthus amarus and Viscum album as feed additives on some metabolic indicators and oxidative status of rabbit testis and brain. Isonitrogenous and isocaloric diets were formulated with 5% supplementation of each of the phytogenic additive to 3 treatment groups and basal diet group.

Results
It was revealed that moringa, mistletoe, and phyllanthus can modulate oxidative status in both the brain and gonads of rabbit bucks through their unique phytochemical compositions, thereby affecting reproductive and cognitive functions. Moringa, rich in crude protein, saponins, glycosides, and steroids, enhances protein and lactate dehydrogenase levels but increases lipid peroxidation in the testis. Mistletoe, with high crude fiber, ash, and antioxidants like flavonoids and tannins, boosts total antioxidant activity in several brain regions and reduces lipid peroxidation, indicating its potential for reducing oxidative stress. Phyllanthus, having the least fiber and ash but effective antioxidant properties, notably affects the oxidative balance in both the testis and brain, with varied impacts on different tissues. The result obtained showed that total antioxidant activity of the left testis was enhanced (p < 0.05) by inclusion of the phytogenic additives, while total antioxidant activity of the right testis in bucks fed on phytogenic additives were similar (p > 0.05) to bucks on basal diet. Bucks fed on moringa and phyllanthus additives had higher (p < 0.05) testicular lipid peroxidation, lowered testicular protein and/or lactate dehydrogenase. Result also shows that lipid peroxidation of hypothalamus, cerebrum, olfactory lobe and cerebellum were lowest in bucks fed on mistletoe, phyllanthus, phyllanthus and phyllanthus, respectively. High catalase activity of optic lobe, olfactory lobe and cerebellum were observed in bucks fed on mistletoe, moringa and mistletoe, respectively, while glutathione peroxidase activity in hypothalamus, cerebrum, olfactory lobe and pineal was enhanced (p < 0.05) in bucks fed on moringa and mistletoe compared to bucks on other treatment.

Conclusion
M. oleifera, P. amarus and V. album leaves as phytogenic feed additives in rabbit diets have negative effect on the metabolic activity of the testis, enhancing antioxidant activity in the brain.
Inflammatory turmoil within: an exploration of autoinflammatory disease genetic underpinnings, clinical presentations, and therapeutic approaches
Authors: Kozu Kátia Tomie , Nascimento Renan Rodrigues Neves Ribeiro do , Aires Patrícia Pontes , Cordeiro Rafael Alves , Moura Thais Costa Lima de , Sztajnbok Flavio Roberto , Pereira Ivanio Alves , Almeida de Jesus Adriana , Perazzio Sandro Félix 
Published: August 2024

AbstractSystemic autoinflammatory diseases (SAIDs) arise from dysregulated innate immune system activity, which leads to systemic inflammation. These disorders, encompassing a diverse array of genetic defects classified as inborn errors of immunity, are significant diagnostic challenges due to their genetic heterogeneity and varied clinical presentations. Although recent advances in genetic sequencing have facilitated pathogenic gene discovery, approximately 40% of SAIDs patients lack molecular diagnoses. SAIDs have distinct clinical phenotypes, and targeted therapeutic approaches are needed. This review aims to underscore the complexity and clinical significance of SAIDs, focusing on prototypical disorders grouped according to their pathophysiology as follows: (i) inflammasomopathies, characterized by excessive activation of inflammasomes, which induces notable IL-1β release; (ii) relopathies, which are monogenic disorders characterized by dysregulation within the NF-κB signaling pathway; (iii) IL-18/IL-36 signaling pathway defect-induced SAIDs, autoinflammatory conditions defined by a dysregulated balance of IL-18/IL-36 cytokine signaling, leading to uncontrolled inflammation and tissue damage, mainly in the skin; (iv) type I interferonopathies, a diverse group of disorders characterized by uncontrolled production of type I interferons (IFNs), notably interferon α, β, and ε; (v) anti-inflammatory signaling pathway impairment-induced SAIDs, a spectrum of conditions characterized by IL-10 and TGFβ anti-inflammatory pathway disruption; and (vi) miscellaneous and polygenic SAIDs. The latter group includes VEXAS syndrome, chronic recurrent multifocal osteomyelitis/chronic nonbacterial osteomyelitis, Schnitzler syndrome, and Still’s disease, among others, illustrating the heterogeneity of SAIDs and the difficulty in creating a comprehensive classification. Therapeutic strategies involving targeted agents, such as JAK inhibitors, IL-1 blockers, and TNF inhibitors, are tailored to the specific disease phenotypes.
Internal rotational patellar resection and patella alta induced patellar maltracking in total knee arthroplasty: intraoperative measurement of the patellofemoral pressure
Authors: Yasuma Sanshiro , Kato Sakurako , Usami Takuya , Hattori Yusuke , Joyo Yuji , Shiraga Hiroo , Nozaki Masahiro , Murakami Hideki , Waguri-Nagaya Yuko 
Published: August 2024

Abstract
Background
Anterior knee pain due to patellar maltracking following total knee arthroplasty (TKA) reduces patients’ satisfaction. This study aimed to determine the patellofemoral pressure (PFP) in patients with favorable patellar tracking (FT) and impaired patellar tracking (IT) following TKA, the factors causing patellar maltracking, and the effect of lateral retinacular release (LRR) on patients with IT.

Methods
Forty-four patients with varus knee osteoarthritis undergoing cruciate-retaining TKA were enrolled. After component implantation, patients with a separation of ≥ 2 mm of the patellar medial facet from the medial femoral trochlea throughout knee range of motion were classified into the IT group; meanwhile, the others were classified into the FT group. PFP was measured intraoperatively in three phases: (1) with the resurfaced patella (RP); (2) with the resurfaced patella and knee (RPK); and (3) when LRR was performed in IT (post-LRR). The PFPs at 0°, 90°, 120°, and 135° knee flexion were compared between FT and IT using the Mann–Whitney U test. Pairwise comparison of the PFP in IT between RPK and post-LRR was performed using the Wilcoxon signed-rank test. Correlations between PFP and pre- and postoperative radiographic parameters, such as hip–knee–ankle angle, lateral distal femoral angle, medial proximal tibial angle, anterior femoral offset, Insall–Salvati ratio (ISR), patellar tilt, and patellar resection angle (PRA), were evaluated using Spearman’s rank correlation coefficients.

Results
High lateral PFP in the knee flexion position led to patellar maltracking. Patients with IT (n = 24) had higher lateral and lower medial PFP than did patients with FT (n = 20) at 90°, 120°, and 135° knee flexion in RP and RPK. LRR in IT reduced the lateral PFP in the knee flexion position. PRA and ISR were correlated with the lateral PFP at no less than 90° in RP and RPK.

Conclusions
This study demonstrated that internal rotational patellar resection, which resulted in a thick medial patellar remnant and a thin lateral counterpart, and patella alta were the causative factors of high lateral PFP, which induced patellar maltracking after TKA. Surgeons should avoid internal rotational patellar resection to achieve FT and perform LRR in patients with patellar maltracking.
Unpacking the nuances: an exploratory multilevel analysis on the operationalization of integrated STEM education and student attitudinal change
Authors: Hiwatig Benny Mart R. , Roehrig Gillian H. , Rouleau Mark D. 
Published: August 2024

AbstractIntegrated STEM education (iSTEM) is recognized for its potential to improve students’ scientific and mathematical knowledge, as well as to nurture positive attitudes toward STEM, which are essential for motivating students to consider STEM-related careers. While prior studies have examined the relationship between specific iSTEM activities or curricula and changes in student attitudes, research is lacking on how the aspects of iSTEM are operationalized and their influence on shifts in student attitudes towards STEM, especially when considering the role of demographic factors. Addressing this gap, our study applied multilevel modeling to analyze how different iSTEM aspects and demographic variables predict changes in student attitudes. Drawing on data from two five-year NSF-funded projects, we evaluated pre- and post-attitude survey responses from 948 students. Our analysis identified two key iSTEM aspects—relating content to students’ lives and engagement in engineering design—that significantly influence positive attitude change. The results highlight the importance of curriculum relevance and hands-on, problem-solving activities in shaping student attitudes. However, the impact of these instructional strategies varies across demographic groups. The study’s insights into the differential impact of iSTEM aspects on diverse student groups provide actionable guidance for educators, curriculum developers, and policymakers aiming to enhance STEM learning experiences and outcomes.
Momentousness of integration of the high-resolution computed tomography scoring systems with pulmonary artery systolic pressure measurement for inference of idiopathic pulmonary fibrosis severity
Authors: Ragaee Sara Mahmoud , Shamsy Alyaa Mohamed Ali EL , Shweel Mohamed Abdel Ghany , Kasem Ahmed Hussein , Mahmoud Hassan Ali Ahmed 
Published: August 2024

Abstract
Background
The importance of high-resolution computed tomography (HRCT) in the diagnosis of idiopathic interstitial pneumonia (IIP) is being recognized more and more. Pulmonary arterial hypertension is common in patients with idiopathic pulmonary fibrosis, the presence of PAH is linked to higher mortality rates and might explain why some people who otherwise have normal pulmonary function are displaying symptoms of deterioration. The aim of this work was to find out the potency of integration of high-resolution computed tomography (HRCT) scoring system with pulmonary artery systolic pressure (PASP) for ascertaining the disease severity of patients with idiopathic pulmonary fibrosis and to predict the prognosis for proper management, along with incorporation of the key disparity of different HRCT scoring systems for the ILD.

Results
This prospective study included 80 patients, who came with idiopathic pulmonary fibrosis. A strong positive correlation (r = 0.989) was noted between total lung zone HRCT score and PASP, pulmonary artery size, grades of dyspnea and PCO2. Nonetheless, total lung zone HRCT score proved to have good correlation and inversely proportional to (r = − 0.71) 6MWT, PO2, SPO2, FVC and FEV1/FVC. We utilized a ROC curve to identify the optimal cutoff value in HRCT scoring systems, for detecting pulmonary hypertension associated with the IPF. The sensitivity and specificity of Warrick score cutoff value was higher compared to that of the HRCT scoring system-based grading scale. Both HRCT scoring systems attained high correlation coefficient factors with various incorporated parameters in the study.

Conclusion
We can safely say that in order to reveal the severity and prognosis of idiopathic pulmonary fibrosis, a high-resolution computed tomography scoring system should be incorporated with echocardiography measurement of pulmonary artery systolic pressure —which proved to be the most potent predictive factor; especially when measured after a specific HRCT score cutoff value—followed by (FEV1/FVC), the 6MWT and the severity of dyspnea. Their integration collectively will aid in precise management and follow-up of IPF cases. Both HRCT scoring systems proved to be potent and perfect for short-term interval follow-up, yet the Warrick score was easier and faster to use and attained relatively increased sensitivity and specificity for the cutoff value of the pulmonary hypertension detection compared to the HRCT scoring system-based grading scale.
α-Amylase, α-glucosidase and aldose reductase inhibitory and molecular docking studies on Tinospora cordifolia (Guduchi) leaf extract
Authors: Bhosale Hemlata Janardhan , Mamdapure Shailesh Vaijeenath , Panchal Ramdas Balaji , Dhuldhaj Umesh Pravin 
Published: August 2024

Abstract
Background
Type II diabetes mellitus is posing a severe health threat throughout the globe due to its associated pathophysiological risks and high mortality rate. Carbohydrate catabolic enzymes, including α-amylase, α-glucosidase and aldose reductase, play an important role in the development of diabetes. The natural or synthetic inhibitors of these enzymes are crucial in reducing diabetes and its related complications. Tinosporacordifolia is a plant of great significance in Ayurveda due to its unique biological activities, including anti-diabetic properties. The present study aims to identify the active constituents of T. cordifolia leaves and evaluate the in vitro inhibitory potential of its ethanol extract constituents against α-amylase, α-glucosidase and aldose reductase activities.

Results
The ethanolic leaf extract of T. cordifolia inhibited the activities of α-amylase, α-glucosidase and aldose reductase in a dose-dependent manner. It was on par with the standard inhibitors acarbose and quercetin. At 5 mg/ml, the noted % inhibition values of extract were 69.27 ± 0.17, 67.8 ± 0.26 and 62.55 ± 0.24, respectively, for α-amylase, α-glucosidase and aldose reductase. Using GC-MS analysis, neophytadiene, γ-sitosterol, phytol, phytyl palmitate, and phytyl acetate were identified as prominent constituents of the ethanolic extract. Based on molecular docking and ADME analysis, γ-sitosterol was found as the major reactive phytoconstituent, which showed the highest inhibitory potential against α-amylase, α-glucosidase and aldose reductase activities.

Conclusions
The present study identified γ-sitosterol as triplet inhibitor of α-amylase, α-glucosidase and aldose reductase and affirmed the ethno-medicinal significance of T. cordifolia leaves in the development of new anti-diabetic leads.
Combined Transcriptome and Circulating Tumor DNA Longitudinal Biomarker Analysis Associates With Clinical Outcomes in Advanced Solid Tumors Treated With Pembrolizumab
Authors: Hernando-Calvo Alberto , Yang S.Y. Cindy , Vila-Casadesús Maria , Han Ming , Liu Zhihui Amy , Berman A Hal K. , Spreafico Anna , Razak Albiruni Abdul , Lheureux Stephanie , Hansen Aaron R. , Lo Giacco Deborah , Abbas-Aghababazadeh Farnoosh , Matito Judith , Haibe-Kains Benjamin , Pugh Trevor J. , Bratman Scott V. , Aleshin Alexey , Berche Roger , Saavedra Omar , Garralda Elena , Elston Sawako , Siu Lillian L. , Ohashi Pamela S. , Vivancos Ana , Bedard Philippe L. 
Published: August 2024

PURPOSE Immune gene expression signatures are emerging as potential biomarkers for immunotherapy (IO). VIGex is a 12-gene expression classifier developed in both nCounter (Nanostring) and RNA sequencing (RNA-seq) assays and analytically validated across laboratories. VIGex classifies tumor samples into hot, intermediate-cold (I-Cold), and cold subgroups. VIGex-Hot has been associated with better IO treatment outcomes. Here, we investigated the performance of VIGex and other IO biomarkers in an independent data set of patients treated with pembrolizumab in the INSPIRE phase II clinical trial (ClinicalTrials.gov identifier: NCT02644369 ). MATERIALS AND METHODS Patients with advanced solid tumors were treated with pembrolizumab 200 mg IV once every 3 weeks. Tumor RNA-seq data from baseline tumor samples were classified by the VIGex algorithm. Circulating tumor DNA (ctDNA) was measured at baseline and start of cycle 3 using the bespoke Signatera assay. VIGex-Hot was compared with VIGex I-Cold + Cold and four groups were defined on the basis of the combination of VIGex subgroups and the change in ctDNA at cycle 3 from baseline (ΔctDNA). RESULTS Seventy-six patients were enrolled, including 16 ovarian, 12 breast, 12 head and neck cancers, 10 melanoma, and 26 other tumor types. Objective response rate was 24% in VIGex-Hot and 10% in I-Cold/Cold. VIGex-Hot subgroup was associated with higher overall survival (OS) and progression-free survival (PFS) when included in a multivariable model adjusted for tumor type, tumor mutation burden, and PD-L1 immunohistochemistry. The addition of ΔctDNA improved the predictive performance of the baseline VIGex classification for both OS and PFS. CONCLUSION Our data indicate that the addition of ΔctDNA to baseline VIGex may refine prediction for IO.
Literacy among American Indians: Levels and Trends from 1900 to 1930 and across Birth Cohorts from 1830 to 1920
Authors: Thornton Arland , Young-DeMarco Linda 
Published: August 2024

Abstract
This article investigates American Indian literacy in the United States. Using 1900 through 1930 decennial census data, literacy levels and trends in reading and writing are documented for this period and for people born from 1820 through 1920, providing a large-scale historical picture of American Indian literacy. The pace and extent of literacy is documented from very low for those born during the early 1800s to fairly universal for those born during the early 1900s. Increases in Native literacy are demonstrated to have been closely related to birth year, with successive birth years having higher levels of literacy. Little evidence was found that increases from 1900 to 1930 happened because people increased their literacy after the school years and as they matured through adulthood. A close birth year level relationship between literacy and one year of school attainment was also found.
The Last Days of the Mosquito Reservation: The Mosquito Indian Diplomatic Mission to Restore the Mosquito Reservation, 1894–1907
Authors: Baracco Luciano 
Published: August 2024

Abstract
Geopolitical changes taking place in late nineteenth-century Central America laid the pathway for Nicaragua’s long-desired incorporation of the autonomous Mosquito Reservation, which was located on its Caribbean Coast. This article brings to light the diplomatic mission undertaken by Mosquito Indians to restore their reservation using rarely accessed British Foreign Office documents. These sources detail the extensive efforts by the last hereditary chief, Robert Henry Clarence, and a small number of Mosquito delegates living in exile in Jamaica, to persuade Great Britain to restore the autonomy granted to them under the Treaty of Managua (1860). Mosquito appeals for British support drew on notions of shared Anglo culture owing to their long historical association with the British and their conversion to Protestant Christianity from the 1840s. The discussion of Mosquito diplomatic correspondence reveals that they possessed a much more sophisticated understanding of notions of nationhood and international law than is commonly acknowledged. The Mosquitos’ association with the British, which had indeed been empowering during the early nineteenth century, proved to be entirely disempowering as the century drew to a close. The establishment of US hegemony over the isthmus had radically transformed Central America’s diplomatic landscape and had undermined Britain’s ability to intervene on behalf of the Mosquito.
da_Tracker: Automated workflow for high throughput single cell and single phagosome tracking in infected cells
Authors: Augenstreich Jacques , Poddar Anushka , Belew Ashton T. , El-Sayed Najib M. , Briken Volker 
Published: August 2024

ABSTRACT
Time-lapse microscopy has emerged as a crucial tool in cell biology, facilitating a deeper understanding of dynamic cellular processes. While existing tracking tools have proven effective in detecting and monitoring objects over time, the quantification of signals within these tracked objects often faces implementation constraints. In the context of infectious diseases, the quantification of signals at localized compartments within the cell and around intracellular pathogens can provide even deeper insight into the interactions between the pathogen and host cell organelles. Existing quantitative analysis at a single-phagosome level remains limited and dependent on manual tracking methods. We developed a near-fully automated workflow that performs with limited bias, high-throughput cell segmentation and quantitative tracking of both single cell and single bacterium/phagosome within multi-channel, z-stack, time-lapse confocal microscopy videos. We took advantage of the PyImageJ library to bring Fiji functionality into a Python environment and combined deep-learning-based segmentation from Cellpose with tracking algorithms from Trackmate. The ‘da_tracker’ workflow provides a versatile toolkit of functions for measuring relevant signal parameters at the single-cell level (such as velocity or bacterial burden) and at the single-phagosome level (i.e. assessment of phagosome maturation over time). Its capabilities in both single-cell and single-phagosome quantification, its flexibility and open-source nature should assist studies that aim to decipher for example the pathogenicity of bacteria and the mechanism of virulence factors that could pave the way for the development of innovative therapeutic approaches.
Effectiveness of kiwi fruit in treating constipation - narrative review
Authors: Bednarczyk Paulina , Malaka Ewa Katarzyna , Maślankiewicz Maria , Nowoświat Patrycja , Bado Michał , Goncerz Michał , Bilecki Krzysztof , Duda Weronika , Muzyk Magdalena , Pilawska Sandra Agnieszka 
Published: August 2024

Introduction: Constipation is a widespread digestive issue characterized by infrequent bowel movements and difficulty in passing stools. While conventional treatments include dietary fiber, laxatives, and lifestyle changes, recent studies have suggested that kiwifruit may offer a natural and effective alternative. This narrative review evaluates the efficacy of kiwifruit in managing and alleviating symptoms of constipation.
Aim of the study: The primary aim of this study is to synthesize existing research on the effectiveness of kiwifruit in treating constipation. By examining various clinical trials and observational studies, the review aims to elucidate the therapeutic benefits and mechanisms of action of kiwifruit in improving bowel function.
Materials and Methods: A comprehensive literature search was performed using databases such as PubMed, Scopus, and Google Scholar. Data were extracted and analyzed regarding the frequency of bowel movements, stool consistency, and overall gastrointestinal health following kiwifruit consumption.
Results: The review identified that regular consumption of kiwifruit significantly enhances bowel function in both constipated and non-constipated individuals. Clinical trials consistently report increased stool frequency, softer stool consistency, and reduced colonic transit time. The beneficial effects are attributed to the high fiber content of kiwifruit and the presence of the enzyme actinidin, which promotes digestion and bowel movements. Kiwifruit was found to be well-tolerated with minimal adverse effects.
Conclusion:Kiwifruit appears to be a promising natural remedy for constipation, providing substantial improvements in bowel movement regularity and stool characteristics. Its high fiber content and unique enzymatic properties contribute to its effectiveness. Further research is recommended to explore the long-term benefits and potential applications of different varieties of kiwifruit in diverse patient populations.