bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evolutionary and molecular analysis of Dof transcription factors identified a conserved motif for intercellular protein trafficking
Authors: Chen Huan , Ahmad Munawar , Rim Yeonggil , Lucas William J. , Kim Jae‐Yean 
Published: March 2013

Summary

Cell‐to‐cell trafficking of transcription factors (TFs) has been shown to play an important role in the regulation of plant developmental events, but the evolutionary relationship between cell‐autonomous and noncell‐autonomous (NCA) TFs remains elusive.

AtDof4.1, named INTERCELLULAR TRAFFICKING DOF 1 (ITD1), was chosen as a representative NCA member to explore this evolutionary relationship. Using domain structure–function analyses and swapping studies, we examined the cell‐to‐cell trafficking of plant‐specific Dof TF family members across Arabidopsis and other species.

We identified a conserved intercellular trafficking motif (ITM) that is necessary and sufficient for selective cell‐to‐cell trafficking and can impart gain‐of‐function cell‐to‐cell movement capacity to an otherwise cell‐autonomous TF. The functionality of related motifs from Dof members across the plant kingdom extended, surprisingly, to a unicellular alga that lacked plasmodesmata. By contrast, the algal homeodomain related to the NCA KNOX homeodomain was either inefficient or unable to impart such cell‐to‐cell movement function.

The Dof ITM appears to predate the evolution of selective plasmodesmal trafficking in the plant kingdom, which may well have acted as a molecular template for the evolution of Dof proteins as NCA TFs. However, the ability to efficiently traffic for KNOX homeodomain (HD) proteins may have been acquired during the evolution of early nonvascular plants.
Firm Recruitment Behaviour: Sequential or Non‐sequential Search?
Authors: Ommeren Jos van , Russo Giovanni 
Published: March 2013

AbstractIn the extensive job search literature, studies assume either sequential or non‐sequential search. This article introduces a novel method to test the hypothesis that firms search sequentially based on the relationship between the number of rejected job applicants and the number of filled vacancies. We distinguish between ten different search methods. For most search methods, including methods that rely on social networks and temporary help agencies, we find that sequential search cannot be rejected. However, when firms use advertising or public/private employment agencies, sequential search is rejected. Hence, we find that both forms of search are relevant for our understanding of the labour market. Further, the form of search is closely related to the search method used.
Crude oil discovery and exploitation: the bane of manufacturing sector development in an oil‐rich country, Nigeria
Authors: Edo Samson E. 
Published: March 2013

AbstractThis paper investigates the impact of crude oil boom on the economy of Nigeria particularly the manufacturing sector. In the study, a descriptive analysis of the three major sectors of the economy is undertaken followed by the formulation of a vector autoregression model depicting the relationship existing among the sectors—resource, manufacturing and service. The model was subsequently estimated using appropriate techniques such as unit root test, cointegration test, causality test, variance decomposition and parametric estimation. The unit root and cointegration tests reveal that the data series employed are reliable and the three sectors are most likely to converge in the long run, which augurs well for policy making. The causality test, variance decomposition and parametric estimation reveal that the oil boom led to significant stagnation in the manufacturing sector and a marginal decline in the service sector. The growth of manufacturing sector of Nigeria has thus been severely impaired by the oil boom. In light of this, adequate policy measures need to be taken to resuscitate the manufacturing sector. These measures may include attracting more foreign investment, reducing operating cost in the sector, developing local sources of raw materials, and allocation of more funds from the crude oil revenue to assist the sector. These policy measures may not only resuscitate the manufacturing sector, they would also accelerate growth of the economy as a whole.
Implementation of a transfusion algorithm to reduce blood product utilization in pediatric cardiac surgery
Authors: Whitney Gina , Daves Suanne , Hughes Alex , Watkins Scott , Woods Marcella , Kreger Michael , Marincola Paula , Chocron Isaac , Donahue Brian 
Published: March 2013

SummaryAimThe goal of this project is to measure the impact of standardization of transfusion practice on blood product utilization and postoperative bleeding in pediatric cardiac surgery patients.BackgroundTransfusion is common following cardiopulmonary bypass (CPB) in children and is associated with increased mortality, infection, and duration of mechanical ventilation. Transfusion in pediatric cardiac surgery is often based on clinical judgment rather than objective data. Although objective transfusion algorithms have demonstrated efficacy for reducing transfusion in adult cardiac surgery, such algorithms have not been applied in the pediatric setting.MethodsThis quality improvement effort was designed to reduce blood product utilization in pediatric cardiac surgery using a blood product transfusion algorithm. We implemented an evidence‐based transfusion protocol in January 2011 and monitored the impact of this algorithm on blood product utilization, chest tube output during the first 12 h of intensive care unit (ICU) admission, and predischarge mortality.ResultsWhen compared with the 12 months preceding implementation, blood utilization per case in the operating room odds ratio (OR) for the 11 months following implementation decreased by 66% for red cells (P = 0.001) and 86% for cryoprecipitate (P < 0.001). Blood utilization during the first 12 h of ICU did not increase during this time and actually decreased 56% for plasma (P = 0.006) and 41% for red cells (P = 0.031), indicating that the decrease in OR transfusion did not shift the transfusion burden to the ICU. Postoperative bleeding, as measured by chest tube output in the first 12 ICU hours, did not increase following implementation of the algorithm. Monthly surgical volume did not change significantly following implementation of the algorithm (P = 0.477). In a logistic regression model for predischarge mortality among the nontransplant patients, after accounting for surgical severity and duration of CPB, use of the transfusion algorithm was associated with a 0.247 relative risk of mortality (P = 0.013).ConclusionsThese results indicate that introduction of an objective transfusion algorithm in pediatric cardiac surgery significantly reduces perioperative blood product utilization and mortality, without increasing postoperative chest tube losses.
Cerebrovascular autoregulation in pediatric moyamoya disease
Authors: Lee Jennifer K. , Williams Monica , Jennings Jacky M. , Jamrogowicz Jessica L. , Larson Abby C. , Jordan Lori C. , Heitmiller Eugenie S. , Hogue Charles W. , Ahn Edward S. 
Published: March 2013

SummaryBackgroundMoyamoya syndrome carries a high risk of cerebral ischemia, and impaired cerebrovascular autoregulation may play a critical role. Autoregulation indices derived from near‐infrared spectroscopy (NIRS) may clarify hemodynamic goals that conform to the limits of autoregulation.ObjectivesThe aims of this pilot study were to determine whether the NIRS‐derived indices could identify blood pressure ranges that optimize autoregulation and whether autoregulatory function differs between anatomic sides in patients with unilateral vasculopathy.MethodsPediatric patients undergoing indirect surgical revascularization for moyamoya were enrolled sequentially. NIRS‐derived autoregulation indices, the cerebral oximetry index (COx) and the hemoglobin volume index (HVx), were calculated intraoperatively and postoperatively to measure autoregulatory function. The 5‐mmHg ranges of optimal mean arterial blood pressure (MAPOPT) with best autoregulation and the lower limit of autoregulation (LLA) were identified.ResultsOf seven enrolled patients (aged 2–16 years), six had intraoperative and postoperative autoregulation monitoring and one had only intraoperative monitoring. Intraoperative MAPOPT was identified in six (86%) of seven patients with median values of 60–80 mmHg. Intraoperative LLA was identified in three (43%) patients with median values of 55–65 mmHg. Postoperative MAPOPT was identified in six (100%) of six patients with median values of 70–90 mmHg. Patients with unilateral disease had higher intraoperative HVx (P = 0.012) on side vasculopathy.ConclusionsNIRS‐derived indices may identify hemodynamic goals that optimize autoregulation in pediatric moyamoya.
Long‐term Follow‐up of Cervical Facet Medial Branch Radiofrequency Treatment With the Single Posterior‐lateral Approach: An Exploratory Study
Authors: van Eerd Maarten , de Meij Nelleke , Dortangs Erik , Kessels Alfons , van Zundert Jan , Lataster Arno , Patijn Jacob , van Kleef Maarten 
Published: March 2013

AbstractBackgroundOver 50% of patients presenting to pain clinic with neck pain have the cervical facet joints as the source of pain. Radiofrequency (RF) treatment of the medial branch, innervating the facet joint, is a therapeutic option. The objectives of this study were to evaluate the therapeutic effect and its duration of RF treatment, using the single posterior‐lateral approach in patients suffering from facet joint degeneration and to identify predictors for a long‐term effect.MethodsOf the 130 consecutive patients with axial neck pain referred to the University Pain Center Maastricht, 67 fulfilled the inclusion criteria. The therapeutic effect was measured using the Patients' Global Impression of Change (PGIC) scale. Retrospective data were made complete using newly collected PGIC follow‐up data. A Kaplan–Meier curve evaluated the long‐term therapeutic effect. Possible predictors of outcome were evaluated.ResultsTwo patients refused to participate and in the remaining 65 patients, overall pain relief was reported in 55.4% at 2‐month follow‐up. Moderately, important change of improvement and substantial change of improvement were seen in 50.8% of patients. At 3‐year follow‐up, 30% still reported pain reduction. Spinal treatment level was the only predictor found.ConclusionsRadiofrequency treatment of the cervical facet joints using a single posterior‐lateral approach is a promising technique in patients with chronic neck pain due to facet degeneration. The short‐term and long‐term therapeutic effects of this intervention justify a randomized controlled trial to estimate the efficacy of cervical facet joint RF treatment in a chronic neck pain population.
Molecular evidence for phytosiderophore‐induced improvement of iron nutrition of peanut intercropped with maize in calcareous soil
Authors: XIONG HONGCHUN , KAKEI YUSUKE , KOBAYASHI TAKANORI , GUO XIAOTONG , NAKAZONO MIKIO , TAKAHASHI HIROKAZU , NAKANISHI HIROMI , SHEN HONGYUN , ZHANG FUSUO , NISHIZAWA NAOKO K. , ZUO YUANMEI 
Published: March 2013

AbstractPeanut/maize intercropping is a sustainable and effective agroecosystem that evidently enhances the Fe nutrition of peanuts in calcareous soils. So far, the mechanism involved in this process has not been elucidated. In this study, we unravel the effects of phytosiderophores in improving Fe nutrition of intercropped peanuts in peanut/maize intercropping. The maize ys3 mutant, which cannot release phytosiderophores, did not improve Fe nutrition of peanut, whereas the maize ys1 mutant, which can release phytosiderophores, prevented Fe deficiency, indicating an important role of phytosiderophores in improving the Fe nutrition of intercropped peanut. Hydroponic experiments were performed to simplify the intercropping system, which revealed that phytosiderophores released by Fe‐deficient wheat promoted Fe acquisition in nearby peanuts and thus improved their Fe nutrition. Moreover, the phytosiderophore deoxymugineic acid (DMA) was detected in the roots of intercropped peanuts. The yellow stripe1‐like (YSL) family of genes, which are homologous to maize yellow stripe 1 (ZmYS1), were identified in peanut roots. Further characterization indicated that among five AhYSL genes, AhYSL1, which was localized in the epidermis of peanut roots, transported Fe(III)–DMA. These results imply that in alkaline soil, Fe(III)–DMA dissolved by maize might be absorbed directly by neighbouring peanuts in the peanut/maize intercropping system.
Pediatric post‐transplant metabolic syndrome: New clouds on the horizon
Authors: Nobili Valerio , de Ville de Goyet Jean 
Published: March 2013

AbstractLiver transplantation (LT) is a standard treatment for children with end‐stage liver disease, standing at more than 90% survival rate after one yr, and at over a 70% survival rate after five yr. The majority of transplanted children enjoy an excellent quality of life but complications can occur in the long term, and can develop subclinically in otherwise well children; there are various underestimated nutritional and metabolic aspects, including the so‐called post‐transplant metabolic syndrome (PTMS). During the post‐transplant period, the use of immunosuppressants, corticosteroids, calcineurin inhibitors, and the presence of risk factors, including non‐alcoholic fatty liver disease (NAFLD), and kidney and bone complications have been largely implicated in PTMS development. Strategies to reduce the progression of PMTS should include careful screening of patients for diabetes, dyslipidemia, and obesity, and to support weight reduction with a carefully constructed program, particularly based on diet modification and exercise. With early identification and appropriate and aggressive management, excellent long‐term health outcomes and acceptable graft survival can be achieved.
Testosterone Influences Water Transport in Porcine Granulosa Cells
Authors: Grzesiak M , Williams L , Luck MR 
Published: March 2013

ContentsThe development of antral ovarian follicles entails fluid accumulation, but the mechanisms regulating water flux are unknown. Aquaporins are small, integral membrane proteins facilitating passive movement of water, some of which are known to be regulated by steroid hormones. The aim of this study was to determine whether testosterone (T) influences water transport in porcine granulosa cells. To assess water movement, the swelling of granulosa cells when moved from isotonic (319 mOsm) to hypotonic (95 mOsm) medium was measured after 12‐hour pre‐incubation in the presence of either testosterone (T), the antiandrogen 2‐hydroxyflutamide (HF) or HF and T together. Pre‐incubation with T increased the swelling of granulosa cells (p < 0.01) and this was abolished by HF (p < 0.001). Neither T nor HF affected cells in isotonic medium (319 mOsm). The results indicate that T acting via intracellular androgen receptors increases water permeability of porcine granulosa cells, probably through the regulation of aquaporin activity.
Religion and the American Landscape
Authors: Pasquier Michael 
Published: March 2013

AbstractThe visual representation of religion in the American landscape is practically everywhere. From the rural countryside to the urban enclave and from the colonial period to the present, inhabitants of what would become the United States have modified the natural landscape to reflect their religious perspectives in a changing world. Churches, temples, schools, monuments, roadside shrines, cemeteries—these are all things we observe in the landscape that physically and visually mark a place as religious and provide us with cues for further exploration of the religious people behind the objects, past and present. Though lacking a coherent disciplinary field, the study of America’s religious landscape brings together scholars from history, geography, philosophy, and architecture to identify how religion both alters and is altered by the natural and built environment throughout American history. Taken together, the three interrelated themes of “nature,”“structure,” and “memory” speak to the manifest ways in which religious adherents have invested meaning in the natural landscape and constructed sacred sites upon that same landscape.
Understanding the Demonologies of the Dead Sea Scrolls: Accomplishments and Directions for the Future
Authors: Reynolds Bennie H. 
Published: March 2013

AbstractThis essay examines scholarship on the demonology of the Dead Sea Scrolls (DSS). It places a special focus on how the notions of evil spirits found in the Book of Watchers (1 Enoch 1–36) were adopted, adapted, and reconfigured in various ways in the scrolls. Concepts crucial to many groups within Second Temple Judaism (impurity, dualism, apocalypticism, and monotheism) functioned to influence how the evolving concept of demonic beings was received and deployed in Hellenistic Jewish thought. The DSS provide the largest and most significant data set for analyzing these developments. The essay concludes by suggesting a critical methodological issue for future research on Jewish demonology.
‘Mapping a Field: Forms of Hindu Religiosity in Malaysia and Singapore’
Authors: Sinha Vineeta 
Published: March 2013

AbstractThis short paper surveys the scholarship on Hinduism as a dynamic, ‘living’ religious tradition in two nation‐states in contemporary Southeast Asia, i.e., Malaysia and Singapore. Since the early decades of the 20th century, archaeologists and historians have expressed enthusiastic curiosity about longer‐term historical interactions between India and parts of Southeast Asia, in particular the question of how to account for the presence of ‘Indic traces’ in these regions. The various debates about the modes and methods through which Hindu and Buddhist elements ‘originally’ interfaced with and were embedded in Southeast Asian civilizational contexts continue to engage scholars. In contrast, relatively less scholarly effort has been directed at theorizing contemporary manifestations of Hinduism in Southeast Asian regions. Although the field is a marginal one, there is a body of scholarship that has documented the religious landscape of Hindu communities in some Southeast Asian regions. I begin with a general observation and a crucial starting point for this paper: that Hinduism as a practiced religion is vigorously sustained in, and has a legitimate and legal presence within broadly secular, state frameworks of Singapore and Malaysia. Certainly, there are important differences in the ways that Hindu communities negotiate these socio‐cultural and political landscapes, yet I argue that a common discussion is possible and allows me to abstract shared trends as well as highlight divergences in the religious landscape of these two Hindu communities.
The best way possible! A fieldwork study outlining expectations and needs for nursing of patients in endoscopy facilities for short‐term stay
Authors: Bundgaard Karin , Nielsen Karl Brian , Sørensen Erik Elgaard , Delmar Charlotte 
Published: March 2013

This paper is a descriptive study of nursing in facilities for short‐term stay, aiming to outline the expectations and needs for nursing of patients undergoing gastroscopy in outpatient endoscopy clinics. Existing research finds it important to meet patients' expectations and needs for help to get through a procedure in the best and safest way possible. Despite recent years' focus on patient expectations, little attention has been paid to understanding the patients' distinct expectations and needs for nursing in the context of facilities for short‐term stay. A fieldwork study influenced by practical ethnographic principles was performed in high‐technology endoscopy outpatient clinics during 2008 – 2010. Data were collected using triangulation of participant observation for 12 weeks including participant reports and semi‐structured interviews with eight patients and four nurses. The expectations and needs for nursing of patients undergoing gastroscopy were related to two main areas, summarized by the categories: ‘Nervousness and anxiety’ and ‘Maintaining control’. The former concerned how patients managed their nervousness and anxiety and was described differently in terms of ‘Getting it over with’, ‘The meaning of words’ and ‘Taking precautions’. The latter ‘Maintaining control’ concerned how patients in different ways managed to maintain control over their situation and was described in terms of ‘Being informed’, ‘Others are in the same “boat”’ and ‘Being proactive’. The study concludes that nervousness and anxiety are expressed differently in patients undergoing gastroscopy and that patients have individual ways of claiming their right to elements of control over the situation and the course of gastroscopy. In order for nursing in endoscopy settings to be tailored to the individual patient, it must be adapted to the individual patient's ways of managing nervousness and anxiety as well as ways of claiming control.
The 14bp‐deletion allele in the HLA‐G gene confers susceptibility to the development of hepatocellular carcinoma in the Brazilian population
Authors: Teixeira A. C. , Mendes‐Junior C. T. , Souza F. F. , Marano L. A. , Deghaide N. H. S. , Ferreira S. C. , Mente Ê. D. , Sankarankutty A. K. , Elias‐Junior J. , Castro‐e‐Silva O. , Donadi E. A. , Martinelli A. L. C. 
Published: March 2013

AbstractHuman leukocyte antigen‐G (HLA‐G) is a nonclassical HLA class I molecule involved in tumor escape mechanisms. Considering that the HLA‐G 14bp insertion/deletion polymorphism is located at the 3′ untranslated region (3′UTR) in exon 8, and since it has been associated with the magnitude of HLA‐G production, we studied the association of 14bp insertion/deletion polymorphism with the risk of developing hepatocellular carcinoma (HCC). A total of 109 HCC patients followed at the University Hospital, Faculty of Medicine of Ribeirão Preto, São Paulo, Brazil, and 202 healthy controls from the same geographic area were genotyped for the 14bp insertion/deletion polymorphism using polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis. Compared to controls, the frequency of the 14bp deletion allele was overrepresented in HCC patients (65% versus 56%, respectively, P = 0.0326). The 14bp deletion conferred an odds ratio (OR) of 1.46 [95% confidence interval (CI): 1.04–2.05]. Similarly, the deletion/deletion genotype was marginally overrepresented in HCC patients (45% versus 35% in controls, P = 0.0871), conferring an OR of 1.54 (95% CI: 0.96–2.48). The frequencies of the deletion/insertion or insertion/insertion genotypes observed in patients were not statistically different from those observed in controls (P > 0.05). Our results suggest that the 14bp‐deletion allele in HLA‐G gene is associated with HCC susceptibility in a Brazilian population.