bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality of life of older people in an urban slum of India
Authors: Ghosh Somenath , Bandyopadhyay Sanjib , Bhattacharya Saikat , Misra Raghunath , Das Saibal 
Published: December 2014

AbstractObjectivesThe aim of this study was to examine different domains of quality of life (QOL) and their relationship to sociodemographic characteristics among older people in an urban slum in India.Materials and MethodsA cross‐sectional study was conducted (n = 120). Selected individuals were interviewed, and their QOL was assessed by the World Health Organization Quality of Life‐BREF questionnaire. Statistical analysis was then performed.ResultsOf those included in the study population, 61.7% were men, all were Hindus, 46.7% were members of the general caste, 19.2% were illiterate, 35% were unemployed, and 72.5% lived with their joint family (i.e. extended family). Mean scores in each domain studied did not significantly differ between the sexes, age groups, castes, and family types. Subjects with more education, who were married, and with greater income had significantly better QOL scores. Mean scores were also better in certain domains among persons who had their own income and who resided with their children.ConclusionHaving low education, being single, lacking personal income, and not living with their children significantly reduced QOL in the elderly subjects. Attention should be given to these factors to help elderly individuals age in a healthy manner.
Determinants of oral health‐related quality of life of the institutionalized elderly
Authors: Zenthöfer Andreas , Rammelsberg Peter , Cabrera Tomas , Schröder Johannes , Hassel Alexander Jochen 
Published: December 2014

AbstractBackgroundDiminished oral health of the institutionalized elderly has frequently been reported. This is not only of significance with regard to nutrition and general health, but it can also affect oral health‐related quality of life. This paper evaluates the effects of oral and general conditions on oral health‐related quality of life for the institutionalized elderly, as measured by the Geriatric Oral Health Assessment Index (GOHAI).Materials and MethodsNinety‐four residents of four long‐term care homes in southern Germany agreed to participate in this study, and interviews were conducted using the GOHAI questionnaire. The Revised Oral Health Assessment Guide was applied to allocate participants into two groups: satisfactory oral health and poor oral health. To evaluate the effects of age, gender, number of chronic diseases, frequently taken drugs, care level, Mini‐Mental State Examination score, periodontitis (for participants with their own teeth), pressures caused by prostheses, and type of denture worn, a linear regression model with the total GOHAI score was calculated with these determinants.ResultsThe mean ± SD GOHAI score was 53.3 ± 6.2 in the satisfactory oral health group and 48.7 ± 7.3 in the poor oral health group. Statistical analysis showed that the GOHAI scores were significantly different in satisfactory oral health and poor oral health groups (P = 0.030) and were affected by care level (P = 0.008) and type of dentures worn (P = 0.006).ConclusionsWithin the limitations of this study, it can be concluded that oral health‐related quality of life of the institutionalized elderly is low and is positively associated with better oral health.
Genome Sequence of Bacillus simplex Strain P558, Isolated from a Human Fecal Sample
Authors: Croce Olivier , Hugon Perrine , Lagier Jean-Christophe , Bibi Fehmida , Robert Catherine , Azhar Esam Ibraheem , Raoult Didier , Fournier Pierre-Edouard 
Published: December 2014

ABSTRACT

Bacillus simplex
strain P558 was isolated from a fecal sample of a 25-year-old Saudi male. We sequenced the 5.98-Mb genome of the strain and compared it to that of
B. simplex
strain 1NLA3E
.
Draft Genome Sequence and Annotation of the Entomopathogenic Bacterium Photorhabdus luminescens LN2, Which Shows Nematicidal Activity against
Heterorhabditis bacteriophora
H06 Nematodes
Authors: Qiu Xuehong , Zhan Zu-Bing , Yan Xun , Han Richou 
Published: December 2014

ABSTRACT

We present here the 5.6-Mb genome sequence of
Photorhabdus luminescens
strain LN2, a Gram-negative bacterium that is a symbiont of
Heterorhabditis indica
LN2 and shows nematicidal activity against
Heterorhabditis bacteriophora
H06 nematodes.
Whole-Genome Sequence of Marine Bacterium Phaeodactylibacter xiamenensis Strain KD52, Isolated from the Phycosphere of Microalga
Phacodactylum tricornutum
Authors: Chen Zhangran , Lei Xueqian , Li Yi , Zhang Jingyan , Zhang Huajun , Yang Luxi , Zheng Wei , Xu Hong , Zheng Tianling 
Published: December 2014

ABSTRACT

Phaeodactylibacter xiamenensis
KD52 is a novel bacterium isolated from a culture of the alga
Phaeodactylum tricornutum
in Xiamen, Fujian Province, China. Here, we present the first draft genome sequence of this strain, which will provide an opportunity to further understand the functional genes related to signing for nutrition from the host algae and the molecular mechanisms underlying its beneficial properties.
Draft Genome Sequence of the Mannitol-Producing Strain Lactobacillus mucosae CRL573
Authors: Bleckwedel Juliana , Terán Lucrecia C. , Bonacina Julieta , Saavedra Lucila , Mozzi Fernanda , Raya Raúl R. 
Published: December 2014

ABSTRACT

Lactobacillus mucosae
CRL573, isolated from child fecal samples, efficiently converts fructose and/or sucrose into the low-calorie sugar mannitol when cultured in modified MRS medium at pH 5.0. Also, the strain is capable of producing bacteriocin. The draft genome sequence of this strain with potential industrial applications is presented here.
Draft Genome Sequence of Propane- and Butane-Oxidizing Actinobacterium Rhodococcus ruber IEGM 231
Authors: Ivshina Irena B. , Kuyukina Maria S. , Krivoruchko Anastasiya V. , Barbe Valérie , Fischer Cécile 
Published: December 2014

ABSTRACT

We report a draft genome sequence of
Rhodococcus ruber
IEGM 231, isolated from a water spring near an oil-extracting enterprise (Perm region, Russian Federation). This sequence provides important insights into the genetic mechanisms of propane and
n
-butane metabolism, organic sulfide and beta-sitosterol biotransformation, glycolipid biosurfactant production, and heavy metal resistance in actinobacteria.
Improving management of paracetamol overdose: a software solution
Authors: Stewart Michael , Robinson Alison , Curran Andy 
Published: December 2014

ObjectiveParacetamol overdose is a common presentation to UK emergency departments, and national audits consistently identify shortfalls in the management of these patients. This study assesses the potential of a web-based computer application to improve the assessment and treatment of these patients.MethodsA retrospective cohort of patients was identified and their actual management and that advised by the software were assessed against current guidelines.ResultsThe application gave correct advice in all cases, significantly outperforming both actual treatment when the patients originally presented, and national performance based on previous College of Emergency Medicine audits.ConclusionsAdvice from custom-written software outperforms actual treatment of patients presenting after paracetamol overdose.
Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus
Authors: Liu Ming-Fei , Wang Chrong-Reen 
Published: December 2014

ObjectivesTh17/IL-17 dysregulation is involved in human autoimmunity, and recent evidence suggests the character of long-lived differentiated memory cells in Th17. By directly measuring the peripheral blood mononuclear cells (PBMC), elevated circulating frequencies of Th17 cells have been reported in systemic lupus erythematosus (SLE) with inconsistent results regarding the correlation with disease activities. In this study, the association between circulating Th17 frequencies and disease activities or laboratory parameters was examined in flow cytometer-sorted CD45RO-positive memory CD4 T cells from SLE.MethodsPBMC samples were obtained from 48 female lupus patients and another 48 age- and sex-matched healthy individuals. We examined frequencies of Th17 cells by sorting the purified CD4 T cells bearing the CD45RO marker, followed by intracellular IL-17A staining after in vitro activation. Frequencies of Th1 and TFoxp3cells were also measured by intracellular IFN-γ and Foxp3 staining, respectively. The SLE disease activity index (SLEDAI) and other laboratory parameters were further correlated with frequencies of different T cell subsets.ResultsIn SLE, increased frequencies of Th17 cells were found with a positive correlation in SLEDAI. Higher frequencies of Th17 cells were found in lupus nephritis. There was a positive correlation between frequencies of Th17 cells and daily proteinuria amount.ConclusionsBy examining the sorted CD45RO-positive memory CD4 T cells, we confirm the dysregulation of Th17/IL-17 in SLE, implicating the potential to treat lupus patients with selective IL-17/IL-17R blockades.
Y-stent assisted coiling of bifurcation aneurysms with Enterprise stent: long-term follow-up
Authors: Limbucci Nicola , Renieri Leonardo , Nappini Sergio , Consoli Arturo , Rosi Andrea , Mangiafico Salvatore 
Published: December 2014

BackgroundY-stent assisted coiling has been proposed for the treatment of wide-neck bifurcation aneurysms, but there are many technical variations. We report our single-center experience of Y-stent assisted coiling of bifurcation aneurysms with the closed cell Enterprise stent in order to evaluate the safety and long-term results of this technique. The literature on Y-stenting and its hemodynamic effects are reviewed.MethodsFifty-two consecutive patients with wide-neck bifurcation aneurysms underwent Y-stent assisted coiling with two Enterprise stents. The procedure was completed in 48 cases (92.3%) and technical failure occurred in 4 cases (7.3%). Cases performed with other stents were excluded. All procedures were performed under double antiplatelet therapy. Periodic clinical and neuroradiological follow-up was performed.ResultsMean neuroradiological follow-up time was 26 months. Complete immediate occlusion was obtained in 87.5% of patients. Two remnants had regrown at follow-up and were recoiled, achieving complete occlusion. The late neuroradiological occlusion rate was: complete occlusion 93.6%, neck remnant 4.3%, sac remnant 2.1%. No in-stent stenosis was detected at follow-up. Among the 48 procedures, two complications occurred (4.2%). Mortality was 2.1%. No delayed ischemic stroke occurred.ConclusionsY-stent assisted coiling has a high immediate occlusion rate and very good long-term stability. The procedure is relatively safe, although the complication and mortality rates are not negligible. Two Enterprise stents can be safely used for Y-stenting and, indeed, offer the advantage of easier catheterization, delivery and deployment into distal and tortuous vessels than open cell stents.
Dual Inhibition of Bcr-Abl and Hsp90 by C086 Potently Inhibits the Proliferation of Imatinib-Resistant CML Cells
Authors: Wu Lixian , Yu Jing , Chen Ruijia , Liu Yang , Lou Liguang , Wu Ying , Huang Lisen , Fan Yingjuan , Gao Pinzhang , Huang Meijuan , Wu Yong , Chen Yuanzhong , Xu Jianhua 
Published: December 2014

Abstract
Purpose: Although tyrosine kinase inhibitors (TKI) such as imatinib provide an effective treatment against Bcr-Abl kinase activity in the mature cells of patients with chronic myelogenous leukemia (CML), TKIs probably cannot eradicate the leukemia stem cell (LSC) population. Therefore, alternative therapies are required to target both mature CML cells with wild-type (WT) or mutant Bcr-Abl and LSCs. To investigate the effect of C086, a derivative of curcumin, on imatinib-resistant cells, we explored its underlying mechanisms of Bcr-Abl kinase and heat shock protein 90 (Hsp90) function inhibition.
Experimental Design: Biochemical assays were used to test ABL kinase activity; fluorescence measurements using recombinant NHsp90, Hsp90 ATPase assay, immunoprecipitation, and immunoblotting were applied to examine Hsp90 function. Colony-forming unit, long-term culture-initiating cells (LTC-IC), and flow cytometry were used to test CML progenitor and stem cells.
Results: Biochemical assays with purified recombinant Abl kinase confirmed that C086 can directly inhibit the kinase activity of Abl, including WT and the Q252H, Y253F, and T315I mutations. Furthermore, we identified C086 as a novel Hsp90 inhibitor with the capacity to disrupt the Hsp90 chaperone function in CML cells. Consequently, it inhibited the growth of both imatinib-sensitive and -resistant CML cells. Interestingly, C086 has the capacity to inhibit LTC-ICs and to induce apoptosis in both CD34+CD38+ and CD34+CD38− cells in vitro. Moreover, C086 could decrease the number of CD45+, CD45+CD34+CD38+, and CD45+CD34+CD38− cells in CML NOD-SCID mice.
Conclusions: Dual suppression of Abl kinase activity and Hsp90 chaperone function by C086 provides a new therapeutic strategy for treating Bcr-Abl–induced leukemia resistant to TKIs. Clin Cancer Res; 21(4); 833–43. ©2014 AACR.
Molecular Pathways: Targeting NRG1 Fusions in Lung Cancer
Authors: Fernandez-Cuesta Lynnette , Thomas Roman K. 
Published: December 2014

Abstract
The four members of the ERBB (HER) family of transmembrane receptor tyrosine kinases are frequently activated in cancer by several mechanisms, such as mutation, amplification, or autocrine ligand–receptor stimulation. We recently identified gene fusions involving the ERBB ligand gene, NRG1, which represent a novel mechanism for ERBB pathway deregulation. These fusions lead to expression and presentation of the EGF-like domain of NRG1 on the cell surface, which binds to ERBB3 in an autocrine and juxtacrine manner, thus inducing the formation of ERBB2–ERBB3 heterodimers, and subsequent activation of the PI3K–AKT and MAPK signaling pathways. These fusion genes were exclusively detected in lung adenocarcinomas of never smokers of the invasive mucinous subtype, which usually presents as a multifocal and unresectable disease, for which no effective treatment exists. Considering the large amount of drugs that target ERBB2 (HER2) and ERBB3 (HER3), and which are currently in different stages of clinical development, detecting and targeting NRG1 fusions in invasive mucinous lung adenocarcinomas may represent a therapeutic opportunity for this aggressive disease. Clin Cancer Res; 21(9); 1989–94. ©2014 AACR.
Molecular Pathways: Aspirin and Wnt Signaling—A Molecularly Targeted Approach to Cancer Prevention and Treatment
Authors: Gala Manish K. , Chan Andrew T. 
Published: December 2014

Abstract
The anti-inflammatory properties of aspirin have resulted in its widespread use as an analgesic, antipyretic, and cardioprotective agent. Beyond these applications, multiple observational studies and randomized controlled trials have demonstrated a chemopreventative role for aspirin, particularly in the development of colorectal neoplasia. Given the critical importance of Wnt dysregulation in colorectal carcinogenesis, the interplay between aspirin and canonical Wnt signaling has become a focus of investigation. These studies have illuminated our understanding of the anticancer mechanisms of aspirin, yielding the identification of potential biomarkers for which aspirin's chemopreventative efficacy can be safely optimized into routine clinical practice and providing leads into the discovery of novel preventive and therapeutic targets. In this review, we summarize key experimental and clinical studies of this interaction, as well as highlighting future strategies to advance their clinical translation. Clin Cancer Res; 21(7); 1543–8. ©2014 AACR.
CDK 4/6 Inhibitor Palbociclib (PD0332991) in Rb+ Advanced Breast Cancer: Phase II Activity, Safety, and Predictive Biomarker Assessment
Authors: DeMichele Angela , Clark Amy S. , Tan Kay See , Heitjan Daniel F. , Gramlich Kristi , Gallagher Maryann , Lal Priti , Feldman Michael , Zhang Paul , Colameco Christopher , Lewis David , Langer Melissa , Goodman Noah , Domchek Susan , Gogineni Keerthi , Rosen Mark , Fox Kevin , O'Dwyer Peter 
Published: December 2014

AbstractPurpose: The G1–S checkpoint of the cell cycle is frequently dysregulated in breast cancer. Palbociclib (PD0332991) is an oral inhibitor of CDK4/6. Based upon preclinical/phase I activity, we performed a phase II, single-arm trial of palbociclib in advanced breast cancer.Experimental Design: Eligible patients had histologically confirmed, metastatic breast cancer positive for retinoblastoma (Rb) protein and measureable disease. Palbociclib was given at 125 mg orally on days 1 to 21 of a 28-day cycle. Primary objectives were tumor response and tolerability. Secondary objectives included progression-free survival (PFS) and assessment of Rb expression/localization, KI-67, p16 loss, and CCND1 amplification.Results: Thirty-seven patients were enrolled; 84% hormone-receptor (HR)+/Her2−, 5% HR+/Her2+, and 11% HR−/Her2−, with a median of 2 prior cytotoxic regimens. Two patients had partial response (PR) and 5 had stable disease ≥ 6 months for a clinical benefit rate (CBR = PR + 6moSD) of 19% overall, 21% in HR+, and 29% in HR+/Her2− who had progressed through ≥2 prior lines of hormonal therapy. Median PFS overall was 3.7 months [95% confidence interval (CI), 1.9–5.1], but significantly longer for those with HR+ versus HR− disease (P = 0.03) and those who had previously progressed through endocrine therapy for advanced disease (P = 0.02). Grade 3/4 toxicities included neutropenia (51%), anemia (5%), and thrombocytopenia (22%). Twenty-four percent had treatment interruption and 51% had dose reduction, all for cytopenias. No biomarker identified a sensitive tumor population.Conclusions: Single-agent palbociclib is well tolerated and active in patients with endocrine-resistant, HR+, Rb-positive breast cancer. Cytopenias were uncomplicated and easily managed with dose reduction. Clin Cancer Res; 21(5); 995–1001. ©2014 AACR.
Space–Time Structure of Diabatic Heating in Monsoon Intraseasonal Oscillation
Authors: Hazra Abheera , Krishnamurthy V. 
Published: December 2014

Abstract
The space–time structure of the leading monsoon intraseasonal oscillation (MISO) in three-dimensional diabatic heating is studied. Using the ERA-Interim data of the European Centre for Medium-Range Weather Forecasts, the diabatic heating data were constructed by the residual method of the thermodynamic equation. The MISO was extracted by applying multichannel singular spectrum analysis on the daily anomalies of three-dimensional diabatic heating over the South Asian monsoon region for the period 1979–2011.The diabatic heating MISO has a period of 45 days, and exhibits eastward propagation in the equatorial Indian and Pacific Oceans and northward propagation over the entire monsoon region. The horizontal structure shows a long tilted band of heating anomalies propagating northeastward. The period, horizontal pattern, and propagation properties of the diabatic heating MISO are similar to those found in precipitation, outgoing longwave radiation, and circulation in earlier studies. The vertical structure of the diabatic heating MISO indicates deep columns, with maximum values at about 450 hPa, propagating northeastward. The vertical structure of the heating anomalies has good correspondence with that of the moisture anomalies but with a phase difference. The moisture anomalies lead the heating anomalies and may provide a preconditioning process for the propagation mechanism. The temperature anomalies also show oscillatory behavior corresponding to the diabatic heating MISO but the phase difference between the two varies from region to region.
A Marine Autonomous Surface Craft for Long-Duration, Spatially Explicit, Multidisciplinary Water Column Sampling in Coastal and Estuarine Systems
Authors: Codiga Daniel L. 
Published: December 2014

AbstractThe Surveying Coastal Ocean Autonomous Profiler (SCOAP) is a large catamaran marine autonomous surface craft (MASC) for unattended weeks-long, spatially explicit, multidisciplinary oceanographic water column profile sampling in coastal/estuarine waterbodies. Material transport rates/pathways, crucial to understanding these ecosystems, are typically poorly known. SCOAP addresses demanding spatiotemporal sampling needs and operational challenges (strong currents, open coastal sea states, complex bathymetry, heavy vessel traffic). Its large size (11-m length, 5-m beam) provides seaworthiness/stability. The average speed of 2.5 m s−1 meets the representative goal to traverse an 18-km transect, sampling 10 min at each of 10 stations 2 km apart, nominally 4 times daily. Efficient hulls and a diesel–electric energy system can provide the needed endurance. The U.S. Coast Guard guidelines are followed: lighting, code flags, the Automatic Identification System (AIS), and collision avoidance regulations (COLREGs)-based collision avoidance (CA) by onboard autonomy software. Large energy reserves obviate low-power optimization of sensors, enabling truly multidisciplinary sampling, and provide on-demand propulsion for effective CA. Vessel stability facilitates high-quality current profile observations and will aid engineering/operation of the planned winched profiling system, performance of an anticipated radar system to detect/track non-AIS vessels, and potential research-quality meteorological sensor operation. A Narragansett Bay test deployment, attended by an escort vessel, met design goals; an unattended open coastal deployment is planned for Rhode Island Sound. Scientific and operational strengths of large catamaran MASCs suggest they could be an important cost-effective complement to other sampling platforms (e.g., improved spatiotemporal coverage and resolution, extending farther inshore, with a broader range of sensors, compared to underwater gliders) in coastal/estuarine waters.
Multiscale EnKF Assimilation of Radar and Conventional Observations and Ensemble Forecasting for a Tornadic Mesoscale Convective System
Authors: Snook Nathan , Xue Ming , Jung Youngsun 
Published: December 2014

Abstract
In recent studies, the authors have successfully demonstrated the ability of an ensemble Kalman filter (EnKF), assimilating real radar observations, to produce skillful analyses and subsequent ensemble-based probabilistic forecasts for a tornadic mesoscale convective system (MCS) that occurred over Oklahoma and Texas on 9 May 2007. The current study expands upon this prior work, performing experiments for this case on a larger domain using a nested-grid EnKF, which accounts for mesoscale uncertainties through the initial ensemble and lateral boundary condition perturbations. In these new experiments, conventional observations (including surface, wind profiler, and upper-air observations) are assimilated in addition to the WSR-88D and the Center for Collaborative Adaptive Sensing of the Atmosphere (CASA) radar data used in the previous studies, better representing meso- and convective-scale features. The relative impacts of conventional and radar data on analyses and forecasts are examined, and biases within the ensemble are investigated.
The new experiments produce a substantially improved forecast, including better representation of the convective lines of the MCS. Assimilation of radar data substantially improves the ensemble precipitation forecast. Assimilation of conventional data together with radar observations substantially improves the forecast of near-surface mesovortices within the MCS, improves forecasts of surface temperature and dewpoint, and imparts a slight but noticeable improvement to short-term precipitation forecasts. Furthermore, ensemble analyses and forecasts are found to be sensitive to the localization radius applied to conventional data within the EnKF.