bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and risk factors for carriage of antimicrobial-resistant Escherichia coli on household and small-scale chicken farms in the Mekong Delta of Vietnam
Authors: Nguyen Vinh Trung , Carrique-Mas Juan J. , Ngo Thi Hoa , Ho Huynh Mai , Ha Thanh Tuyen , Campbell James I. , Nguyen Thi Nhung , Hoang Ngoc Nhung , Pham Van Minh , Wagenaar Jaap A. , Hardon Anita , Thai Quoc Hieu , Schultsz Constance 
Published: March 2015

Abstract

Objectives
To describe the prevalence of antimicrobial resistance among commensal Escherichia coli isolates on household and small-scale chicken farms, common in southern Vietnam, and to investigate the association of antimicrobial resistance with farming practices and antimicrobial usage.

Methods
We collected data on farming and antimicrobial usage from 208 chicken farms. E. coli was isolated from boot swab samples using MacConkey agar (MA) and MA with ceftazidime, nalidixic acid or gentamicin. Isolates were tested for their susceptibility to 11 antimicrobials and for ESBL production. Risk factor analyses were carried out, using logistic regression, at both the bacterial population and farm levels.

Results
E. coli resistant to gentamicin, ciprofloxacin and third-generation cephalosporins was detected on 201 (96.6%), 191 (91.8%) and 77 (37.0%) of the farms, respectively. Of the 895 E. coli isolates, resistance to gentamicin, ciprofloxacin and third-generation cephalosporins was detected in 178 (19.9%), 291 (32.5%) and 29 (3.2%) of the isolates, respectively. Ciprofloxacin resistance was significantly associated with quinolone usage (OR = 2.26) and tetracycline usage (OR = 1.70). ESBL-producing E. coli were associated with farms containing fish ponds (OR = 4.82).

Conclusions
Household and small farms showed frequent antimicrobial usage associated with a high prevalence of resistance to the most commonly used antimicrobials. Given the weak biocontainment, the high prevalence of resistant E. coli could represent a risk to the environment and to humans.
Bactericidal mode of action of bedaquiline
Authors: Hards Kiel , Robson Jennifer R. , Berney Michael , Shaw Lisa , Bald Dirk , Koul Anil , Andries Koen , Cook Gregory M. 
Published: March 2015

Abstract

Objectives
It is not fully understood why inhibiting ATP synthesis in Mycobacterium species leads to death in non-replicating cells. We investigated the bactericidal mode of action of the anti-tubercular F1Fo-ATP synthase inhibitor bedaquiline (Sirturo™) in order to further understand the lethality of ATP synthase inhibition.

Methods
Mycobacterium smegmatis strains were used for all the experiments. Growth and survival during a bedaquiline challenge were performed in multiple media types. A time-course microarray was performed during initial bedaquiline challenge in minimal medium. Oxygen consumption and proton-motive force measurements were performed on whole cells and inverted membrane vesicles, respectively.

Results
A killing of 3 log10 cfu/mL was achieved 4-fold more quickly in minimal medium (a glycerol carbon source) versus rich medium (LB with Tween 80) during bedaquiline challenge. Assessing the accelerated killing condition, we identified a transcriptional remodelling of metabolism that was consistent with respiratory dysfunction but inconsistent with ATP depletion. In glycerol-energized cell suspensions, bedaquiline caused an immediate 2.3-fold increase in oxygen consumption. Bedaquiline collapsed the transmembrane pH gradient, but not the membrane potential, in a dose-dependent manner. Both these effects were dependent on binding to the F1Fo-ATP synthase.

Conclusions
Challenge with bedaquiline results in an electroneutral uncoupling of respiration-driven ATP synthesis. This may be a determinant of the bactericidal effects of bedaquiline, while ATP depletion may be a determinant of its delayed onset of killing. We propose that bedaquiline binds to and perturbs the a-c subunit interface of the Fo, leading to futile proton cycling, which is known to be lethal to mycobacteria.
Impact of the meaningful use incentive program on electronic health record adoption by US children's hospitals
Authors: Nakamura Mari M. , Harper Marvin B. , Castro Allan V. , Yu Feliciano B. , Jha Ashish K. 
Published: March 2015

Abstract
Objective We determined adoption rates of pediatric-oriented electronic health record (EHR) features by US children's hospitals and assessed perceptions regarding the suitability of commercial EHRs for pediatric care and the influence of the meaningful use incentive program on implementation of pediatric-oriented features.
Materials and Methods We surveyed members of the Children's Hospital Association. We measured adoption of 19 pediatric-oriented features and asked whether commercial EHRs include key pediatric-focused capabilities. We inquired about the meaningful use program's relevance to pediatrics and its influence on EHR implementation priorities.
Results Of 164 general acute care children's hospitals, 100 (61%) responded to the survey. Rates of comprehensive (across all pediatric units) adoption ranged from 37% (age-, gender-, and weight-adjusted blood pressure percentiles and immunization contraindication warnings) to 87% (age in appropriate units). Implementation rates for several features varied significantly by children's hospital type. Nearly 60% of hospitals reported having EHRs that do not contain all features essential for high-quality care. A majority of hospitals indicated that the meaningful use program has had no effect on their adoption of pediatric features, while 26% said they have delayed or forgone incorporation of such features because of the program.
Conclusions Children's hospitals are implementing pediatric-focused features, but a sizable proportion still finds their systems suboptimal for pediatric care. The meaningful use incentive program is failing to promote and in some cases delaying uptake of pediatric-oriented features.
The subsurface of Pluto from submillimetre observations
Authors: Greaves J. S. , Whitelaw A. C. M. , Bendo G. J. 
Published: March 2015

Abstract
Surface areas on Pluto change in brightness and colour, at optical to infrared wavelengths, over time-scales as short as years. The subsurface contains a reservoir of frozen volatiles, but little is known about it because Pluto is out of reach for cm-radar. Here we present a 0.85 mm wavelength light curve of the Pluto system, from archival data taken in 1997 August with the SCUBA (Submillimetre Common-User Bolometer Array) camera on the James Clerk Maxwell Telescope (JCMT). This wavelength probes for the first time to just below the skin depth of thermal changes over Pluto's day. The light curve differs significantly from counterparts in the mid- to far-infrared, in a longitude range that is optically dark on Pluto's surface. An estimate from Herschel of the 0.5 mm flux in 2012 is comparable to the mean 0.45 mm flux from SCUBA in 1997, suggesting that layers centimetres below the surface have not undergone any gross temperature change. The longitudes that are relatively submillimetre-faint could have a different emissivity, perhaps with a subsurface layer richer in nitrogen or methane ices than at the surface. The Radio Science Experiment (REX) instrument on New Horizons may be able to constrain physical properties deeper down, as it looks back on Pluto's nightside after the 2015 July flyby.
Refined critical balance in strong Alfvénic turbulence
Authors: Mallet A. , Schekochihin A. A. , Chandran B. D. G. 
Published: March 2015

Abstract
We present numerical evidence that in strong Alfvénic turbulence, the critical balance principle – equality of the non-linear decorrelation and linear propagation times – is scale invariant, in the sense that the probability distribution of the ratio of these times is independent of scale. This result only holds if the local alignment of the Elsasser fields is taken into account in calculating the non-linear time. At any given scale, the degree of alignment is found to increase with fluctuation amplitude, supporting the idea that the cause of alignment is mutual dynamical shearing of Elsasser fields. The scale-invariance of critical balance (while all other quantities of interest are strongly intermittent, i.e. have scale-dependent distributions) suggests that it is the most robust of the scaling principles used to describe Alfvénic turbulence. The quality afforded by situ fluctuation measurements in the solar wind allows for direct verification of this fundamental principle.
The Prognostic Significance of Pretreatment Hematologic Parameters in Patients Undergoing Resection for Colorectal Cancer
Authors: Kozak Margaret M. , von Eyben Rie , Pai Jonathan S. , Anderson Eric M. , Welton Mark L. , Shelton Andrew A. , Kin Cindy , Koong Albert C. , Chang Daniel T. 
Published: March 2015

Objectives:
The prognostic value of several hematologic parameters, including platelet, lymphocyte, and neutrophil counts, has been studied in a variety of solid tumors. In this study, we examined the significance of inflammatory markers and their prognostic implications in patients with colorectal cancer (CRC).

Materials and Methods:
Patients with stage I-III CRC who underwent surgical resection at the Stanford Cancer Institute between 2005 and 2009 were included. Patients were excluded if they did not have preoperative complete blood counts performed within 1 month of surgical resection, underwent preoperative chemotherapy or radiation, had metastatic disease at diagnosis, or had another previous malignancy. We included 129 eligible patients with available preoperative complete blood counts in the final analysis.

Results:
A preoperative neutrophil-to-lymphocyte ratio of>3.3 was significantly associated with worse disease-free (DFS) and overall survival (OS) (P=0.009, 0.003), as was a preoperative lymphocyte-to-monocyte ratio of ≤2.6 (P=0.01, 0.002). Preoperative lymphopenia (P=0.002) was associated with worse OS but not DFS (P=0.09). In addition, preoperative thrombocytosis was associated with worse DFS (P=0.006) and OS (P=0.010). Preoperative leukocytosis was associated with worse OS (P=0.048) but not DFS (P=0.49). Preoperative hemoglobin was neither associated with OS (P=0.24) or DFS (P=0.15).

Conclusions:
Pretreatment lymphopenia, thrombocytosis, a decreased lymphocyte-to-monocyte ratio, and an elevated neutrophil-to-lymphocyte ratio independently predict for worse OS in patients with CRC.
Complexity of dendritic SER increases at enlarging synapses during LTP
Authors: Chirillo Michael A. , Bourne Jennifer N. , Lindsey Laurence F. , Harris Kristen M. 
Published: March 2015

AbstractSmooth endoplasmic reticulum (SER) forms a membranous network that extends throughout neurons. SER regulates intracellular calcium and the posttranslational modification and trafficking of membrane and proteins. As the structure of dendritic SER shifts from a tubular to a more complex, branched form, the movement of membrane cargo slows and delivery to nearby spines increases. Here we discovered changes in the structural complexity of SER that have important functional implications during long-term potentiation (LTP) in adult rat hippocampus. By 2 hours after the induction of LTP with theta-burst stimulation, synapse enlargement was greatest on spines that contained SER. More spines had an elaborate spine apparatus than a simple tubule of SER. The SER in dendritic shafts became more complex beneath spines with both polyribosomes and SER, and less complex along aspiny dendritic regions. The findings suggest that local changes in dendritic SER support enhanced growth of specific synapses during LTP.
Tissue dynamics of the forebrain neural plate
Authors: Young Stephen , Jennings Joel N. , Blanchard Guy B. , Kabla Alexandre J. , Adams Richard J. 
Published: March 2015

AbstractThe forebrain has the most complex shape and structure of the vertebrate brain regions and the mechanisms of its formation remain obscure. Convergence and extension movements are characteristic of the posterior (spinal cord and hindbrain) neural plate (pNP) while tissue deformations and underlying cellular dynamics during the early shaping of the forebrain neural plate (fNP) are undefined. Here, we apply live imaging, automated cell tracking and computational analysis to quantitatively map cell behaviour in the zebrafish fNP. We demonstrate a novel mechanism in which planar cell rearrangements, with a passive signature, are orthogonal to those in the pNP, and cell divisions lacking planar-polarity facilitate thickening from two to three layers. We develop a mechanical model of the fNP in which polarised cell behaviour arises from interactions with dissimilar bordering tissues rather than from intrinsically polarised cells. The model unifiesin vivoobservations and provides a mechanistic understanding of fNP morphogenesis.
Phylogeography by Diffusion on a Sphere
Authors: Bouckaert Remco R. 
Published: March 2015

AbstractTechniques for reconstructing geographical history along a phylogeny can answer many interesting question about the geographical origins of species. Bayesian models based on the assumption that taxa move through a diffusion process have found many applications. However, these methods rely on diffusion processes on a plane, and do not take the spherical nature of our planet in account. This makes it hard to perform analysis that cover the whole world, or do not take in account the distortions caused by projections like the Mercator projection.In this paper, we introduce a Bayesian phylogeographical method based on diffusion on a sphere. When the area where taxa are sampled from is small, a sphere can be approximated by a plane and the model results in the same inferences as with models using diffusion on a plane. For taxa samples from the whole world, we obtain substantial differences. We present an efficient algorithm for performing inference in an Markov Chain Monte Carlo (MCMC) algorithm, and show applications to small and large samples areas.Availability: The method is implemented in the GEO_SPHERE package in BEAST 2, which is open source licensed under LGPL.
Predictors of the integration of Sri Lankan Tamil refugees in Canada: pre-migration adversity, mental health, personal attributes, and post-migration experience
Authors: Beiser Morton , Goodwill Alasdair M. , Albanese Patrizia , McShane Kelly , Kanthasamy Parvathy 
Published: March 2015

Purpose
– Refugees integrate less successfully than other immigrants. Pre-migration stress, mental disorder and lack of human capital are the most popular explanations, but these propositions have received little empirical testing. The current study of Sri Lankan Tamils in Toronto, Canada, examines the respective contributions of pre-migration adversity, human capital, mental health and social resources in predicting integration. The paper aims to discuss these issues.

Design/methodology/approach
– Participants are a probability sample of 1,603 Sri Lankan Tamils living in Toronto, Canada. The team, with a community advisory council, developed structured interviews containing information about pre- and post-migration stressors, coping strategies, and family, community, and institutional support. The questionnaire included the World Health Organization Composite International Diagnostic Interview module for post-traumatic stress disorder (PTSD). Interviews were translated, back-translated and administered by bilingual interviewers.

Findings
– Two dimensions of integration emerged from a factor analysis of integration-related items: economic and psychosocial. Hierarchical multiple regression analyses revealed that PTSD militated against refugee economic integration, whereas pre-migration adversity (but not PTSD) compromised psychosocial integration. On both measures, increasing length of residence in Canada, and gender (male) were predictors of good integration, whereas age at arrival had an inverse relationship with integration. Religiosity had a positive effect on psychosocial integration but a negative effect on economic. Favourable perceptions of the health care system predicted economic integration and non-family support predicted psychosocial integration.

Originality/value
– Results underline the importance of studying integration as a multifaceted phenomenon, help explain why refugees integrate less successfully than other immigrants, and highlight the importance of including mental health and mental health-related issues in integration discourse.
The refugee experience of acquiring a family doctor
Authors: Grant Karen J. , Mayhew Maureen , Mota Lorena , Klein Michael C. , Kazanjian Arminée 
Published: March 2015

Purpose
– The purpose of this paper is to explore refugees’ experiences of the barriers and facilitators involved in finding a regular family doctor.

Design/methodology/approach
– Hermeneutic phenomenology was used to produce an integrated description sensitive to the lifeworlds of refugees who came from multiple cultural perspectives. Participants consisted of refugees from Iran, Afghanistan, Myanmar, Vietnam, and Latin America who arrived in Canada between 2005 and 2007. Texts for analysis came from first language focus group discussions and interviews with the interpreters for those groups.

Findings
– The principal themes that emerged from the experience of barriers were “futility,” “dependence,” and “relevance.” Themes related to the experience of facilitators were “inclusion,” “congruence,” and “benefit to family.” These themes provide key messages about sources of patient decisions to seek or not seek care, not comply, attend irregularly, and not disclose symptoms, which can be used by doctors and other health providers to enhance care planning.

Practical implications
– The factors that facilitate refugees’ access to a regular family doctor have implications for the development of culturally appropriate healthcare information, policies that support adequate interpreter services, and cultural sensitivity training for physicians.

Originality/value
– Previous research documents barriers such as lack of language access, differences in health beliefs, and lack of knowledge about western healthcare systems. However, little is known about how refugees experience these barriers, nor how they overcome them. This study contributes a rich and deeper understanding of how refugees experience these barriers and elucidates factors that facilitate their process of obtaining a regular family doctor.
Portfolios of control in mobile eco-systems: evolution and validation
Authors: Mukhopadhyay Sandip , Bouwman Harry , Jaiswal Mahadeo 
Published: March 2015

Purpose– This paper aims to study the critical role played by interorganizational control mechanisms in a mobile ecosystems and how the portfolios of control evolves when the service moves from an initial idea to a solution that reaches market acceptance. Existing literature provides limited insight into (portfolios of) control mechanisms and how (portfolios of) control dynamically evolve(s) during the various stages of service innovation.Design/methodology/approach– Based on the Resource Dependency Theory (RDT), this study makes use of multiple cases to identify and validate the key roles of behavioural input and output control mechanisms and how they evolve during different phases of service development.Findings– Based on multiple cases, it is concluded that a dominant actor uses portfolios of control to acquire complimentary resources, coordinate interdependence between multiple partners and ensure a favourable value distribution for itself. Behavioural control is used in a limited way during the implementation and commercialisation phases, while input control is mostly used during the development phase and output controls are mostly used during implementation and commercialisation phases. The high occurrence of input control in the development phase ensures the lower occurrence of behavioural controls in the implementation phase. This study is very practical in nature, and it provides important insight on how to develop mobile services in collaboration with other organisations.Research limitations/implications– A limitation of this study is that it is based on two cases in a specific regulatory, cultural and institutional environment, i.e. India, which means that further testing of the propositions, with large-scale samples and within a more international setting, would be required. However, this study does provide some interesting insights that have to be corroborated by additional case studies and a large-scale questionnaire, initially with a focus on India.Originality/value– From an academic perspective, this study examines organisational controls in a less researched yet dynamic services industry, and is one of the first studies that the researchers have come across that uses RDT to explain the dynamics of control in value networks in the mobile industry. This study is also one of the very few to focus on understanding the objectives of the portfolios of control from the perspective of the structural player. As mentioned earlier, research focussing on integrating governance mechanisms and portfolios of control may provide new insights. From a practical perspective, this study may shed light on how to develop mobile services in collaboration with other organisations.
Adoption of technology-based services: the role of customers’ willingness to co-create
Authors: Heidenreich Sven , Handrich Matthias 
Published: March 2015

Purpose
– The purpose of this paper is to develop and empirically evaluate an adoption model for technology-based services (TBS) that integrates a customer’s willingness to co-create (WCC) as mediator complementing the well-known individual differences and innovation characteristics in predicting customer adoption of TBS.

Design/methodology/approach
– The manuscript uses structural equation modeling to analyze survey data from two empirical studies (n=781 and n=181).

Findings
– The empirical results show that WCC represents a key mediator between established antecedent predictors (innovation characteristics and individual differences) and the likelihood of TBS adoption. Additionally, the analysis reveals that WCC can even better explain and predict adoption intention of TBS than the commonly used individual differences and innovation characteristics. Finally, the results also suggest that a lack of customers’ WCC may help to explain persuasion-decision discrepancies within TBS adoption.

Research limitations/implications
– As the data of this manuscript pertains to the mobile apps market, future research might test the modified technology adoption model in other TBS contexts as well. While the studies used cross-sectional data, it would be interesting to assess the differential influence of WCC across the stages in the adoption process using longitudinal data.

Practical implications
– The findings on WCC provide managers with a new set of factors (apart from known antecedent predictors like individual differences and innovation characteristics) to optimize TBS adoption.

Originality/value
– This manuscript is the first to examine an adoption model for TBS that integrates a customer’s WCC. Furthermore, the findings provide first empirical evidence that WCC can help to explain persuasion-decision discrepancies within TBS adoption.