bluuebunny/crossref_metadata_2025_split huggingface dataset:
Host Intestinal Signal-Promoted Biofilm Dispersal Induces Vibrio cholerae Colonization
Authors: Hay Amanda J. , Zhu Jun 
Published: November 2014

ABSTRACT

Vibrio cholerae
causes human infection through ingestion of contaminated food and water, leading to the devastating diarrheal disease cholera.
V. cholerae
forms matrix-encased aggregates, known as biofilms, in the native aquatic environment. While the formation of
V. cholerae
biofilms has been well studied, little is known about the dispersal from biofilms, particularly upon entry into the host. In this study, we found that the exposure of mature biofilms to physiologic levels of the bile salt taurocholate, a host signal for the virulence gene induction of
V. cholerae
, induces an increase in the number of detached cells with a concomitant decrease in biofilm mass. Scanning electron microscopy micrographs of biofilms exposed to taurocholate revealed an altered, perhaps degraded, appearance of the biofilm matrix. The inhibition of protein synthesis did not alter rates of detachment, suggesting that
V. cholerae
undergoes a passive dispersal. Cell-free media from taurocholate-exposed biofilms contains a larger amount of free polysaccharide, suggesting an abiotic degradation of biofilm matrix by taurocholate. Furthermore, we found that
V. cholerae
is only able to induce virulence in response to taurocholate after exit from the biofilm. Thus, we propose a model in which
V. cholerae
ingested as a biofilm has coopted the host-derived bile salt signal to detach from the biofilm and go on to activate virulence.
Universal Response-Adaptation Relation in Bacterial Chemotaxis
Authors: Krembel Anna K. , Neumann Silke , Sourjik Victor 
Published: November 2014

ABSTRACT

The bacterial strategy of chemotaxis relies on temporal comparisons of chemical concentrations, where the probability of maintaining the current direction of swimming is modulated by changes in stimulation experienced during the recent past. A short-term memory required for such comparisons is provided by the adaptation system, which operates through the activity-dependent methylation of chemotaxis receptors. Previous theoretical studies have suggested that efficient navigation in gradients requires a well-defined adaptation rate, because the memory time scale needs to match the duration of straight runs made by bacteria. Here we demonstrate that the chemotaxis pathway of
Escherichia coli
does indeed exhibit a universal relation between the response magnitude and adaptation time which does not depend on the type of chemical ligand. Our results suggest that this alignment of adaptation rates for different ligands is achieved through cooperative interactions among chemoreceptors rather than through fine-tuning of methylation rates for individual receptors. This observation illustrates a yet-unrecognized function of receptor clustering in bacterial chemotaxis.
Evaluation of the accuracy in detecting cervical lesions by nurses versus doctors using a stationary colposcope and Gynocular in a low-resource setting
Authors: Nessa Ashrafun , Roy Joya Shree , Chowdhury Most Afroza , Khanam Quayuma , Afroz Romena , Wistrand Charlotte , Thuresson Marcus , Thorsell Malin , Shemer Isaac , Wikström Shemer Elisabeth Andrea 
Published: November 2014

ObjectivesEvaluation of the performance of VIA (visual inspection with acetic acid) trained nurses to learn colposcopy and the Swede score method to detect cervical lesions by using stationary colposcope or a portable, hand-held colposcope; the Gynocular, as compared to doctors.DesignA crossover randomised clinical trial.SettingThe Colposcopy Clinic of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, Bangladesh.Participants932 women attending the clinic as either screening naïve for VIA screening (404) or women referred as VIA positive (528) from other VIA screening centres in the Dhaka region.InterventionVIA trained nurses were trained on-site in colposcopy and in the Swede score systematic colposcopy method. The Swede score grade cervical acetowhiteness, margins plus surface. vessel pattern, lesion size and iodine staining. The women were randomised to start the examination by either a stationary colposcope or the Gynocular. Swede scores were first obtained by a nurse and the same patient was equally evaluated by a doctor.Primary and secondary outcome measuresAgreement between nurses and doctors in Swede scores was evaluated using the weighted κ statistic for the Gynocular and standard colposcope. The ability to predict CIN 2+ (CIN 2, CIN 3 and invasive cervical cancer) using Swede scores was evaluated using receiver-operating characteristic curves.ResultsThe Swede scores obtained by nurses and doctors using the Gynocular and stationary colposcope showed high agreement with a κ statistic of 0.858 and 0.859, respectively, and no difference in detecting cervical lesions in biopsy. Biopsy detected CIN 2+ in 39 (4.2%) women.ConclusionsOur study showed that VIA nurses can perform colposcopy. There was no significant differences compared to doctors in detecting cervical lesions by stationary colposcope or the Gynocular using the Swede score system. Swede scores obtained by nurses using the Gynocular could offer an accurate cervical diagnostic approach in low resource settings.Trial registration numberISRCTN53264564.
Mental symptoms in patients with cardiac symptoms and normal coronary arteries
Authors: Christoph Marian , Christoph Antje , Dannemann Stephanie , Poitz David , Pfluecke Christian , Strasser Ruth H , Wunderlich Carsten , Koellner Volker , Ibrahim Karim 
Published: November 2014

ObjectivesPatients with chest pain and normal coronary arteries often suffer from physical and psychological symptoms. Therefore, this study aimed to examine the incidence of mental symptoms in patients with angiographic exclusion of a coronary heart disease.DesignIn 253 patients with angiographic exclusion of a coronary heart disease the type and intensity of their symptoms were evaluated before and after coronary angiography. In addition, the incidence of psychopathological symptoms was quantified by standardised questionnaires such as general anxiety and depression (HADS), heart-focused anxiety (CAQ), hypochondria (Whiteley Index) and somatoform disorder (SOMS) and quality of life (SF-12). Finally, the incidence of psychological symptoms in these patients was compared to the incidence in the normal population.ResultsDespite the absence of a coronary artery disease, 70% of patients continue to suffer from cardiac symptoms. The incidence of general anxiety was increased by 37% in women and by 22% in men in comparison to the normal population. Heart-focused anxiety was raised by 27%. Somatoform disorder appeared 120% more often in patients after cardiac catheterisation in comparison to the normal population. In addition, the incidence of hypochondria was elevated by 68% in patients after coronary angiography compared to normal population. This increased appearance of psychological symptoms was reflected in a significantly lower quality of life (SF-12) in patients with inconspicuous coronary angiography.ConclusionsPatients with cardiac symptoms and normal coronary arteries more often suffer from mental symptoms in comparison to the healthy population.
Appreciating loess landscapes through history: the basis of modern loess geotourism in the Vojvodina region of North Serbia
Authors: Vasiljević Djordjije A. , Marković Slobodan B. , Vujičić Miroslav D. 
Published: November 2014

Abstract

Loess is wind-blown sediment that covers extensive areas in the middle latitudes. Much of the loess in Eastern and Central–Eastern Europe has been redeposited by the River Danube and its tributaries. The case study area (Vojvodina region) encompasses the confluence area of the Danube, Sava and Tisa rivers. This region includes the most complete and the thickest loess–palaeosol sequences found in Europe, a valuable record of palaeo-climates over the past two million years only recognized in the closing decades of the twentieth century. Long before then however, several enthusiasts, engineers and travellers recognized and appreciated loess as a significant natural phenomenon. Among them was Luigi Ferdinando Marsigli (1658–1730) who gave the first scientific description of European loess in his outstanding multivolume work
Danubius Pannonico Mysicus
where he drew and explained notable loess–palaeosol exposures along the Danube River. Many other loess observations were also recorded by a number of international travellers, whose illustrated travelogues (mainly published in the nineteenth century) mentioned and illustrated loess observations along the Danube and its major tributaries. This chapter explores the interplay of scientific loess research and its geo-historical literary aspects as the foundations of modern loess geotourism.
MDL4BMF
Authors: Miettinen Pauli , Vreeken Jilles 
Published: November 2014

Matrix factorizations—where a given data matrix is approximated by a product of two or more factor matrices—are powerful data mining tools. Among other tasks, matrix factorizations are often used to separate global structure from noise. This, however, requires solving the “model order selection problem” of determining the proper rank of the factorization, that is, to answer where fine-grained structure stops, and where noise starts.
Boolean Matrix Factorization (BMF)—where data, factors, and matrix product are Boolean—has in recent years received increased attention from the data mining community. The technique has desirable properties, such as high interpretability and natural sparsity. Yet, so far no method for selecting the correct model order for BMF has been available. In this article, we propose the use of the Minimum Description Length (MDL) principle for this task. Besides solving the problem, this well-founded approach has numerous benefits; for example, it is automatic, does not require a likelihood function, is fast, and, as experiments show, is highly accurate.
We formulate the description length function for BMF in general—making it applicable for any BMF algorithm. We discuss how to construct an appropriate encoding: starting from a simple and intuitive approach, we arrive at a highly efficient data-to-model--based encoding for BMF. We extend an existing algorithm for BMF to use MDL to identify the best Boolean matrix factorization, analyze the complexity of the problem, and perform an extensive experimental evaluation to study its behavior.
Evaluating Phase Change Memory for Enterprise Storage Systems
Authors: Kim Hyojun , Seshadri Sangeetha , Dickey Clement L. , Chiu Lawrence 
Published: November 2014

Storage systems based on Phase Change Memory (PCM) devices are beginning to generate considerable attention in both industry and academic communities. But whether the technology in its current state will be a commercially and technically viable alternative to entrenched technologies such as flash-based SSDs remains undecided. To address this, it is important to consider PCM SSD devices not just from a device standpoint, but also from a holistic perspective.
This article presents the results of our performance study of a recent all-PCM SSD prototype. The average latency for a 4KiB random read is 6.7μs, which is about 16× faster than a comparable eMLC flash SSD. The distribution of I/O response times is also much narrower than flash SSD for both reads and writes. Based on the performance measurements and real-world workload traces, we explore two typical storage use cases: tiering and caching. We report that the IOPS/$ of a tiered storage system can be improved by 12--66% and the aggregate elapsed time of a server-side caching solution can be improved by up to 35% by adding PCM.
Our results show that (even at current price points) PCM storage devices show promising performance as a new component in enterprise storage systems.
Checking the Integrity of Transactional Mechanisms
Authors: Fryer Daniel , Qin Mike , Sun Jack , Lee Kah Wai , Brown Angela Demke , Goel Ashvin 
Published: November 2014

Data corruption is the most common consequence of file-system bugs. When such corruption occurs, offline check and recovery tools must be used, but they are error prone and cause significant downtime. Previously we showed that a runtime checker for the Ext3 file system can verify that metadata updates are consistent, helping detect corruption in metadata blocks at transaction commit time. However, corruption can still occur when a bug in the file system’s transactional mechanism loses, misdirects, or corrupts writes. We show that a runtime checker must enforce the atomicity and durability properties of the file system on every write, in addition to checking transactions at commit time, to provide the strong guarantee that every block write will maintain file system consistency.
We identify the invariants that need to be enforced on journaling and shadow paging file systems to preserve the integrity of committed transactions. We also describe the key properties that make it feasible to check these invariants for a file system. Based on this characterization, we have implemented runtime checkers for Ext3 and Btrfs. Our evaluation shows that both checkers detect data corruption effectively, and they can be used during normal operation with low overhead.
Prevention of Skin Carcinogenesis by the β-Blocker Carvedilol
Authors: Chang Andy , Yeung Steven , Thakkar Arvind , Huang Kevin M. , Liu Mandy M. , Kanassatega Rhye-Samuel , Parsa Cyrus , Orlando Robert , Jackson Edwin K. , Andresen Bradley T. , Huang Ying 
Published: November 2014

AbstractThe stress-related catecholamine hormones and the α- and β-adrenergic receptors (α- and β-AR) may affect carcinogenesis. The β-AR GRK/β-arrestin biased agonist carvedilol can induce β-AR–mediated transactivation of the EGFR. The initial purpose of this study was to determine whether carvedilol, through activation of EGFR, can promote cancer. Carvedilol failed to promote anchorage-independent growth of JB6 P+ cells, a skin cell model used to study tumor promotion. However, at nontoxic concentrations, carvedilol dose dependently inhibited EGF-induced malignant transformation of JB6 P+ cells, suggesting that carvedilol has chemopreventive activity against skin cancer. Such effect was not observed for the β-AR agonist isoproterenol and the β-AR antagonist atenolol. Gene expression, receptor binding, and functional studies indicate that JB6 P+ cells only express β2-ARs. Carvedilol, but not atenolol, inhibited EGF-mediated activator protein-1 (AP-1) activation. A topical 7,12-dimethylbenz(α)anthracene (DMBA)-induced skin hyperplasia model in SENCAR mice was utilized to determine the in vivo cancer preventative activity of carvedilol. Both topical and oral carvedilol treatment inhibited DMBA-induced epidermal hyperplasia (P &lt; 0.05) and reduced H-ras mutations; topical treatment being the most potent. However, in models of established cancer, carvedilol had modest to no inhibitory effect on tumor growth of human lung cancer A549 cells in vitro and in vivo. In conclusion, these results suggest that the cardiovascular drug carvedilol may be repurposed for skin cancer chemoprevention, but may not be an effective treatment of established tumors. More broadly, this study suggests that β-ARs may serve as a novel target for cancer prevention. Cancer Prev Res; 8(1); 27–36. ©2014 AACR.
Hypertensive Renal Injury Is Associated With Gene Variation Affecting Immune Signaling
Authors: Braun Michael C. , Herring Stacy M. , Gokul Nisha , Monita Monique , Bell Rebecca , Zhu Yaming , Gonzalez-Garay Manuel L. , Wenderfer Scott E. , Doris Peter A. 
Published: November 2014

Background—
The spontaneously hypertensive rat (SHR) strain exists in lines that contrast strongly in susceptibility to renal injury in hypertension. These inbred lines share common ancestry, and only 13% of their genomes arise from different ancestors.

Methods and Results—

We used next gen sequencing to detect natural allelic variation in 5 genes of the immunoreceptor signaling pathway (IgH, Dok3, Src, Syk, and JunD) that arise from different ancestors in the injury-prone SHR-A3 and the resistant SHR-B2 lines. We created an intercross between these lines, and in the F2 progeny, we observed that the inheritance of haplotype blocks containing the SHR-A3 alleles of these 5 genes correlated with increased albuminuria and histological measures of renal injury. To test whether accumulated genetic variation in this pathway may create a therapeutic target in hypertensive renal injury, rats of both lines were treated with the immunosuppressant mycophenolate mofetil (MMF). MMF reduced proteinuria (albumin to creatinine ratio) from 6.6 to 1.2 mg/mg (
P
<0.001) in SHR-A3. Glomerular injury scores were reduced in MMF-treated SHR-A3 from 1.6 to 1.4 (
P
<0.002). Tubulo-interstitial injury was reduced in MMF-treated SHR-A3 from 2.62 to 2.0 (
P
=0.001). MMF treatment also reduced renal fibrosis in SHR-A3 (3.9 versus 2.0;
P
<0.001).

Conclusions—
Polygenic susceptibility to renal injury in hypertension arises in association with genetic variation in genes that participate in immune responses and is dramatically improved by reduction of immune system activity.
Data Fusion Enables Better Recognition of Ceiling and Visibility Hazards in Aviation
Authors: Herzegh Paul , Wiener Gerry , Bateman Richard , Cowie James , Black Jennifer 
Published: November 2014

Abstract
Low cloud ceilings and poor visibility claim the lives of more general aviation (GA) pilots and passengers than any other cause of weather-related GA accidents. Experience shows that instrument-rated pilots as well as those rated only for visual flight are vulnerable to low ceiling and visibility (C&V), making total avoidance the most powerful strategy available to a GA pilot dealing with these hazards. The weather awareness needed for avoidance begins with the recognition of current conditions. This article outlines how fusion of surface, satellite, and terrain data yields a graphical analysis product that enables GA pilots, dispatchers, and weather briefers to better visualize the areal distribution of recent and current C&V conditions across the contiguous U.S. The product is available at www.aviationweather.gov/adds/cv and indicates ceilings less than 1,000 ft above ground level, visibilities less than 3 statute miles, and regions where terrain obscuration is possible. The product is also viewable in the context of interactive geographic information system data via the experimental Helicopter Emergency Medical Services Tool available at http://weather.aero/tools/desktopapps/hemstool. The authors summarize verification results and outline work toward a next-generation product that incorporates the use of model forecast data and weather camera imagery to improve information in data-sparse regions. This next-generation product is in development for initial use in Alaska.
JOYCE: Jülich Observatory for Cloud Evolution
Authors: Löhnert U. , Schween J. H. , Acquistapace C. , Ebell K. , Maahn M. , Barrera-Verdejo M. , Hirsikko A. , Bohn B. , Knaps A. , O’Connor E. , Simmer C. , Wahner A. , Crewell S. 
Published: November 2014

Abstract
The Jülich Observatory for Cloud Evolution (JOYCE), located at Forschungszentrum Jülich in the most western part of Germany, is a recently established platform for cloud research. The main objective of JOYCE is to provide observations, which improve our understanding of the cloudy boundary layer in a midlatitude environment. Continuous and temporally highly resolved measurements that are specifically suited to characterize the diurnal cycle of water vapor, stability, and turbulence in the lower troposphere are performed with a special focus on atmosphere–surface interaction. In addition, instruments are set up to measure the micro- and macrophysical properties of clouds in detail and how they interact with different boundary layer processes and the large-scale synoptic situation. For this, JOYCE is equipped with an array of state-of-the-art active and passive remote sensing and in situ instruments, which are briefly described in this scientific overview. As an example, a 24-h time series of the evolution of a typical cumulus cloud-topped boundary layer is analyzed with respect to stability, turbulence, and cloud properties. Additionally, we present longer-term statistics, which can be used to elucidate the diurnal cycle of water vapor, drizzle formation through autoconversion, and warm versus cold rain precipitation formation. Both case studies and long-term observations are important for improving the representation of clouds in climate and numerical weather prediction models.
Mesoscale Convective Systems and Their Synoptic-Scale Environment in Finland
Authors: Punkka Ari-Juhani , Bister Marja 
Published: November 2014

Abstract
The environments within which high-latitude intense and nonintense mesoscale convective systems (iMCSs and niMCSs) and smaller thunderstorm clusters (sub-MCSs) develop were studied using proximity soundings. MCS statistics covering 8 years were created by analyzing composite radar imagery. One-third of all systems were intense in Finland and the frequency of MCSs was highest in July. On average, MCSs had a duration of 10.8 h and traveled toward the northeast. Many of the linear MCSs had a southwest–northeast line orientation. Interestingly, a few MCSs were observed to travel toward the west, which is a geographically specific feature of the MCS characteristics. The midlevel lapse rate failed to distinguish the environments of the different event types from each other. However, in MCSs, CAPE and the low-level mixing ratio were higher, the deep-layer-mean wind was stronger, and the lifting condensation level (LCL) was lower than in sub-MCSs. CAPE, low-level mixing ratio, and LCL height were the best discriminators between iMCSs and niMCSs. The mean wind over deep layers distinguished the severe wind–producing events from the nonsevere events better than did the vertical equivalent potential temperature difference or the wind shear in shallow layers. No evidence was found to support the hypothesis that dry air at low- and midlevels would increase the likelihood of severe convective winds. Instead, abundant low- and midlevel moisture was present during both iMCS cases and significant wind events. These results emphasize the pronounced role of low- and midlevel moisture on the longevity and intensity of deep moist convection in low-CAPE environments.
Diabetes Connect
Authors: Crabtree Krysia , Sherrer Nathan , Rushton Tullia , Willig Amanda , Agne April , Shelton Tanya , Cherrington Andrea 
Published: November 2014

Purpose The purpose of the study is to explore African American men’s perceptions of how community-based, community-health worker (CHW)–delivered diabetes interventions might best be implemented. Methods Four 90-minute focus groups were guided by a trained moderator with a written guide to facilitate discussion on the topic of diabetes management and preferences for community-based programs. Participants were recruited from the diabetes education database at a safety-net health system in Jefferson County, AL. Two independent reviewers performed content analysis to identify major themes using an iterative, combined deductive and inductive approach. Results There were 25 male participants. Mean years living with diabetes was 9.6 (range, 1-20). Participants demonstrated knowledge of self-management strategies and identified various hardships including emotional and physical manifestations of diabetes, dietary restrictions, and institutional frustrations with the health system that contributed to self-management barriers. Their preferred CHW responsibilities were to educate, hold support groups, help track daily activities, and help find resources. Potential concerns included the need for confidentiality and fears of being stereotyped. Conclusions Participants identified critical self-management strategies but endure hardships that present barriers to daily diabetes management. Preferences for community-based programs and suggested CHW responsibilities could help to overcome many of those barriers by increasing access and providing support.