bluuebunny/crossref_metadata_2025_split huggingface dataset:
Food web structure of the fungivorous insect community on bracket fungi in a Bornean tropical rain forest
Authors: YAMASHITA SATOSHI , ANDO KIYOSHI , HOSHINA HIDETO , ITO NOBORU , KATAYAMA YUJI , KAWANABE MAKOTO , MARUYAMA MUNETOSHI , ITIOKA TAKAO 
Published: May 2015

1. If fungivorous insect diversity is maintained by host specialisation on particular fungi, it should be higher in the tropics than in temperate or boreal regions owing to high macrofungus species diversity.2. To reveal the community and food web structure of fungivorous insects on bracket fungi, fungivorous insects were collected from 427 fruiting bodies belonging to 22 genera throughout the development and deterioration process in a 3‐ha plot of lowland dipterocarp tropical rain forest on Borneo Island.3. Eight hundred and twenty‐nine individuals of 82 coleopteran species in 13 families from 111 fruiting bodies of 15 fungal genera were collected. Tenebrionidae and Staphylinidae were most common. Fifty‐three and 19 insect species were observed onGanodermaandPhellinus, respectively. The numbers of insect species and individuals on a particular fungal genus were positively correlated with the abundance of that fungal genus.4. Quantitative food web analysis revealed a high degree of specialisation at the whole‐community level. At least 65% of insect individuals were observed onGanodermaat every stage of development and deterioration. Diverse insects coexist on one dominant fungal genus,Ganoderma, in contrast to our hypothesis.5. The high abundance ofGanodermafruiting bodies, which lack obvious defences against insect feeding, probably influences the bracket fungus–insect food web in this tropical rainforest.
Ecological management of cereal stemborers in African smallholder agriculture through behavioural manipulation
Authors: MIDEGA CHARLES A. O. , BRUCE TOBY J. A. , PICKETT JOHN A. , KHAN ZEYAUR R. 
Published: May 2015

1. Africa faces serious challenges in feeding its rapidly growing human population owing to the poor productivity of maize and sorghum, the most important staple crops for millions of smallholder farmers in the continent, with yields being among the lowest in the world.2. A complex of lepidopterous stemborers attack cereals in Africa. However, their effective control is difficult, largely as a result of the cryptic and nocturnal habits of moths, and protection provided by host stem for immature pest stages. Moreover, current control measures are uneconomical and impractical for resource‐poor farmers.3. An ecological approach, based on companion planting, known as ‘push–pull’, provides effective management of these pests, and involves combined use of inter‐ and trap cropping systems where stemborers are attracted and trapped on trap plants with added economic value (‘pull’), and are driven away from the cereal crop by antagonistic intercrops (‘push’).4. Novel defence strategies inducible by stemborer oviposition have recently been discovered, leading to the attraction of egg and larval parasitoids, in locally adapted maize lines but not in elite hybrids. We also established that landscape complexity did not improve the ecosystem service of biological control, but rather provided a disservice by acting as a ‘source’ of stemborer pests colonising the crop.5. Here we review and provide new data on the direct and indirect effects of the push–pull approach on stemborers and their natural enemies, including the mechanisms involved, and highlight opportunities for exploiting intrinsic plant defences and natural ecosystem services in pest management in smallholder farming systems in Africa.
Long non‐coding RNA uc.217 regulates neurite outgrowth in dorsal root ganglion neurons following peripheral nerve injury
Authors: Yao Chun , Wang Jing , Zhang Honghong , Zhou Songlin , Qian Tianmei , Ding Fei , Gu Xiaosong , Yu Bin 
Published: May 2015

AbstractThe intrinsic regeneration capacity of dorsal root ganglion (DRG) neurons can be activated after sciatic nerve injury, and peripheral nerve regeneration is a complex process regulated by multiple molecular responses and signaling pathways. Long non‐coding RNAs (lncRNAs) are RNA transcripts > 200 nucleotides in length without protein‐coding potential. They regulate gene expression at epigenetic, transcriptional and post‐transcriptional levels, and are thus involved in many biological processes and human diseases. However, the role and mechanisms of lncRNAs in regulating the responses of DRG neurons to sciatic nerve injury are not fully investigated. We have previously analysed the expression profiles of lncRNAs and mRNAs in L4‐6 DRGs, following rat sciatic nerve transection, by microarray analysis, and constructed a coexpression network of dysregulated lncRNAs and coding genes. In this study, one of these dysregulated lncRNAs, uc.217, was chosen for detailed examination of its expression changes and regulative functions in regenerative DRG neuronal outgrowth. Quantitative real‐time PCR and in situ hybridisation confirmed that the expression of uc.217 was down‐regulated in DRG neurons after sciatic nerve injury. Silencing of uc.217 expression by small interfering RNA could significantly promote neurite outgrowth in cultured DRG neurons. Moreover, bioinformatic analysis and experimental validation were performed to identify several potential targets of uc.217, which were involved in the regulation of DRG neuron outgrowth. Collectively, our results suggested that a new lncRNA, uc.217, played an important regulative role in peripheral nerve regeneration.
Living on the continental shelf edge: habitat use of juvenile shortfin makos Isurus oxyrinchus in the Great Australian Bight, southern Australia
Authors: Rogers Paul J. , Huveneers Charlie , Page Brad , Goldsworthy Simon D. , Coyne Micheal , Lowther Andrew D. , Mitchell James G. , Seuront Laurent 
Published: May 2015

AbstractWe used satellite telemetry data to investigate the movement patterns and habitat use of juvenile shortfin makos Isurus oxyrinchus (Lamnidae) tagged in the Great Australian Bight, southern Australia. Tracking durations ranged from 49–672 days and six deployments were > 1 year. During winter and spring, some shortfin makos migrated to the tropical NE Indian Ocean and Coral Sea, and the Subtropical Front region. One shortfin mako undertook an extended migration of 25 550 km across the Indian Ocean. Areas characterized by sea‐mounts in the NE Indian Ocean, the oceanic Subtropical Front region, and the continental shelf edge (200‐m depth) and slope canyons were visited by several sharks. Juvenile shortfin makos used the outer continental shelf, the shelf edge, the slope and oceanic waters during migrations and mostly exhibited fidelity in the mid‐outer shelf, the shelf edge and slope habitats characterized by high bathymetric relief and oceanographic frontal gradients. Our findings highlighted that the continental shelf and slope and associated submarine canyons of the Great Australian Bight represent ecologically important habitats for juvenile shortfin makos. The findings of this study will be pertinent during future management processes for this highly migratory species in this Southern Hemisphere region.
Effect of bariatric surgery on cardiometabolic risk in elderly patients: A population‐based study
Authors: Batsis John A , Miranda William R , Prasad Chaithra , Collazo‐Clavell Maria L , Sarr Michael G , Somers Virend K , Lopez‐Jimenez Francisco 
Published: May 2015

AimObesity is a major cardiovascular (CV) risk factor. Bariatric surgery (BSx) is an approved therapeutic alternative for class II–III obesity, but little evidence focuses on older adults. We assessed the effect of BSx on cardiometabolic variables and long‐term CV risk in older adults.MethodsWe carried out a population‐based, observational study from 1990–2009, of 40 consecutive elderly (age ≥60 years) residents of Olmsted County, MN, USA, with class II–III obesity treated with BSx at a University‐based, academic health center. Data were obtained from the Rochester Epidemiology Project. Metabolic syndrome (MetS) was defined using American Heart Association/National Heart, Lung, Blood Institute (AHA/NHLBI) criteria (increased triglycerides, low high‐density lipoprotein, increased blood pressure, increased glucose and body mass index as a modified measure of obesity instead of waist circumference). Change in CV risk factors, MetS prevalence, and impact on predicted CV risk using the Framingham risk score was ascertained at 1 year postoperatively and assessed statistically.ResultsMean age and body mass index were 64.4 ± 3.7 and 45.0 ± 6.3 kg/m2, respectively, and 28 out of 40 (70%) were women. One participant died during the 11‐month study period after BSx from respiratory complications related to BSx, and one participant died at 2 years. Percentage of excess weight loss decreased by 57.5% at 1 year. Prevalence 1 year after BSx decreased for diabetes (57.5% to 22.5%; P < 0.03), hypertension 87.5% to 73.7% (P = 0.003), dyslipidemia (80% to 42.5%; P < 0.001) and sleep apnea (62.5% to 23.7%; P < 0.001).MetS prevalence decreased from 80% to 45% (P < 0.002). Baseline risk was 14.1%, which changed at follow up at 8.2%.ConclusionsIn older adults, BSx induces considerable weight loss, improves CV risk factors, decreases MetS prevalence and is an effective treatment in this population. Geriatr Gerontol Int 2016; 16: 618–624.
Aquifer Vulnerability Assessment Based on Sequence Stratigraphic and 39Ar Transport Modeling
Authors: Sonnenborg Torben O. , Scharling Peter B. , Hinsby Klaus , Rasmussen Erik S. , Engesgaard Peter 
Published: May 2015

AbstractA large‐scale groundwater flow and transport model is developed for a deep‐seated (100 to 300 m below ground surface) sedimentary aquifer system. The model is based on a three‐dimensional (3D) hydrostratigraphic model, building on a sequence stratigraphic approach. The flow model is calibrated against observations of hydraulic head and stream discharge while the credibility of the transport model is evaluated against measurements of 39Ar from deep wells using alternative parameterizations of dispersivity and effective porosity. The directly simulated 3D mean age distributions and vertical fluxes are used to visualize the two‐dimensional (2D)/3D age and flux distribution along transects and at the top plane of individual aquifers. The simulation results are used to assess the vulnerability of the aquifer system that generally has been assumed to be protected by thick overlaying clayey units and therefore proposed as future reservoirs for drinking water supply. The results indicate that on a regional scale these deep‐seated aquifers are not as protected from modern surface water contamination as expected because significant leakage to the deeper aquifers occurs. The complex distribution of local and intermediate groundwater flow systems controlled by the distribution of the river network as well as the topographical variation (Tóth 1963) provides the possibility for modern water to be found in even the deepest aquifers.
Graphitization of carbonaceous material in sedimentary rocks on short geologic time‐scales: An example from the Kinsho‐zan area, central Japan
Authors: Mori Natsumi , Wallis Simon , Mori Hiroshi 
Published: May 2015

AbstractInitially amorphous carbonaceous material becomes more crystalline with heating. The structural change depends not only on the maximum attained temperature but also the time‐scale of heating. Raman spectroscopy of natural samples that have been heated for time‐scales of 105 years or greater show that the degree of crystallinity has reached steady‐state. In contrast, laboratory studies show very little change in crystallinity of carbonaceous material (CM) after heating at 1000°C for a time of 3.5 weeks. Better constraints on the time‐scale for crystallization require experiments on time‐scales of years to thousands of years; such long time‐scales can only be derived from natural examples of CM‐bearing rocks that have been heated for a known length of time. Thermal modeling of contact metamorphism developed around a 13 m dike within the Akasaka Limestone in Gifu Prefecture shows the time‐scale of heating is of the order of 1–100 years. Raman spectroscopy reveals a significant increase in the crystallinity of the CM in a region within 3 m from the dike. A comparison between the temperature predicted for the contact aureole and the degree of crystallinity of the carbonaceous material shows that even close to the dike the CM has not reached steady‐state. This change began at over 550°C (modeled temperature) for a time‐scale of heating of a few years. Attaining steady‐state in the crystallization of CM under natural geological condition requires heating on time‐scales greater than about one hundred years. This study shows the utility of using natural laboratory studies to determine the kinetics of CM crystallization in rocks.
Business models and opportunity creation: How IT entrepreneurs create and develop business models under uncertainty
Authors: Ojala Arto 
Published: May 2015

AbstractHow can entrepreneurs develop business models for markets in which the technology is constantly changing—or create business models for markets that do not exist? These are fundamental questions for information technology (IT) entrepreneurs, and for information systems (IS) scholars who seek to develop a theoretical understanding of business models. The case study presented in this paper addressed these questions, demonstrating how a small software firm developed its business model over a 15‐year period in cloud gaming markets. Based on the empirical findings, a preliminary theoretical model is presented. The aim of the model is to increase scholarly understanding of how business models are created and developed in markets in which the future directions of a technology are uncertain. It demonstrates the ways in which a business model may evolve through reassessment and development phases, which can be seen as transition elements linking old and new business models.
‘Porosome’ discovered nearly 20 years ago provides molecular insights into the kiss‐and‐run mechanism of cell secretion
Authors: Jena Bhanu P. 
Published: May 2015

AbstractSecretion is a fundamental cellular process in living organisms, from yeast to cells in humans. Since the 1950s, it was believed that secretory vesicles completely merged with the cell plasma membrane during secretion. While this may occur, the observation of partially empty vesicles in cells following secretion suggests the presence of an additional mechanism that allows partial discharge of intra‐vesicular contents during secretion. This proposed mechanism requires the involvement of a plasma membrane structure called ‘porosome’, which serves to prevent the collapse of secretory vesicles, and to transiently fuse with the plasma membrane (Kiss‐and‐run), expel a portion of its contents and disengage. Porosomes are cup‐shaped supramolecular lipoprotein structures at the cell plasma membrane ranging in size from 15 nm in neurons and astrocytes to 100–180 nm in endocrine and exocrine cells. Neuronal porosomes are composed of nearly 40 proteins. In comparison, the 120 nm nuclear pore complex is composed of >500 protein molecules. Elucidation of the porosome structure, its chemical composition and functional reconstitution into artificial lipid membrane, and the molecular assembly of membrane‐associated t‐SNARE and v‐SNARE proteins in a ring or rosette complex resulting in the establishment of membrane continuity to form a fusion pore at the porosome base, has been demonstrated. Additionally, the molecular mechanism of secretory vesicle swelling, and its requirement for intra‐vesicular content release during cell secretion has also been elucidated. Collectively, these observations provide a molecular understanding of cell secretion, resulting in a paradigm shift in our understanding of the secretory process.
Relationship of social support and decisional conflict to advance directives attitude in Korean older adults: A community‐based cross‐sectional study
Authors: Lee JuHee , Jung Dukyoo , Choi MoonKi 
Published: May 2015

AbstractAimThe aim of this study was to clarify the relationship between social support, decisional conflict, and attitude towards advance directives, and determine whether decisional conflict mediates the relation between social support and advance directives attitude among older adults in South Korea.MethodsIn total, 209 community‐based older adults (mean age, 74.82 years) participated in this cross‐sectional study. Demographic characteristics, self‐perceived health status, social support, decisional conflict, and advance directives attitude were investigated via a structured questionnaire. Data analysis was carried out using Pearson's correlation and path analyses.ResultsThe mean score of advance directives attitude was 48.01 (range, 35–61). Decisional conflict and social support were both significantly related to advance directives attitude (P < 0.001). Additionally, decisional conflict was a mediator between social support and advance directives attitudes.ConclusionThe results confirmed the importance of social support for reducing decisional conflict and encouraging positive attitudes toward advance directives. Future studies are needed to support the development of culturally sensitive educational approaches regarding advance directives for older adults in Korea.
Anterior migration of lateral plate mesodermal cells during embryogenesis of the pufferfish Takifugu niphobles: insight into the rostral positioning of pelvic fins
Authors: Tanaka Mikiko , Yu Reiko , Kurokawa Daisuke 
Published: May 2015

AbstractIn vertebrates, paired appendages (limbs and fins) are derived from the somatic mesoderm subsequent to the separation of the lateral plate mesoderm into somatic and splanchnic layers. This is less clear for teleosts, however, because the developmental processes of separation into two layers and of extension over the yolk have rarely been studied. During teleost evolution, the position of pelvic fins has generally shifted rostrally (Rosen; Nelson, 1982, 1994), although at the early embryonic stage the presumptive pelvic fin cells are initially located near the future anus region – the anterior border of hoxc10a expression in the spinal cord – regardless of their final destination. Our previous studies in zebrafish (abdominal pelvic fins) and Nile tilapia (thoracic pelvic fins) showed that the presumptive pelvic fin cells shift their position with respect to the body trunk after its protrusion from the yolk surface. Furthermore, in Nile tilapia, presumptive pelvic fin cells migrate anteriorly on the yolk surface. Here, we examined the embryonic development of the lateral plate mesoderm at histological levels in the pufferfish Takifugu niphobles, which belongs to the highly derived teleost order Tetraodontiformes, and lacks pelvic fins. Our results show that, in T. niphobles, the lateral plate mesoderm bulges out as two separate layers of cells alongside the body trunk prior to its further extension to cover the yolk sphere. Once the lateral plate mesoderm extends laterally, it rapidly covers the surface of the yolk. Furthermore, cells located near the anterior border of hoxc10a expression in the spinal cord reach the anterior‐most region of the yolk surface. In light of our previous and current studies, we propose that anterior migration of presumptive pelvic fin cells might be required for them to reach the thoracic or more anterior positions as is seen in other highly derived teleost groups.
Genetically induced abnormal cranial development in human trisomy 18 with holoprosencephaly: comparisons with the normal tempo of osteogenic–neural development
Authors: Reid Shaina N. , Ziermann Janine M. , Gondré‐Lewis Marjorie C. 
Published: May 2015

AbstractCraniofacial malformations are common congenital defects caused by failed midline inductive signals. These midline defects are associated with exposure of the fetus to exogenous teratogens and with inborn genetic errors such as those found in Down, Patau, Edwards' and Smith–Lemli–Opitz syndromes. Yet, there are no studies that analyze contributions of synchronous neurocranial and neural development in these disorders. Here we present the first in‐depth analysis of malformations of the basicranium of a holoprosencephalic (HPE) trisomy 18 (T18; Edwards' syndrome) fetus with synophthalmic cyclopia and alobar HPE. With a combination of traditional gross dissection and state‐of‐the‐art computed tomography, we demonstrate the deleterious effects of T18 caused by a translocation at 18p11.31. Bony features included a single developmentally unseparated frontal bone, and complete dual absence of the anterior cranial fossa and ethmoid bone. From a superior view with the calvarium plates removed, there was direct visual access to the orbital foramen and hard palate. Both the eyes and the pituitary gland, normally protected by bony structures, were exposed in the cranial cavity and in direct contact with the brain. The middle cranial fossa was shifted anteriorly, and foramina were either missing or displaced to an abnormal location due to the absence or misplacement of its respective cranial nerve (CN). When CN development was conserved in its induction and placement, the respective foramen developed in its normal location albeit with abnormal gross anatomical features, as seen in the facial nerve (CNVII) and the internal acoustic meatus. More anteriorly localized CNs and their foramina were absent or heavily disrupted compared with posterior ones. The severe malformations exhibited in the cranial fossae, orbital region, pituitary gland and sella turcica highlight the crucial involvement of transcription factors such as TGIF, which is located on chromosome 18 and contributes to neural patterning, in the proper development of neural and cranial structures. Our study of a T18 specimen emphasizes the intricate interplay between bone and brain development in midline craniofacial abnormalities in general.