bluuebunny/crossref_metadata_2025_split huggingface dataset:
NeuO: a Fluorescent Chemical Probe for Live Neuron Labeling
Authors: Er Jun Cheng , Leong Cheryl , Teoh Chai Lean , Yuan Qiang , Merchant Paolomi , Dunn Matthew , Sulzer David , Sames Dalibor , Bhinge Akshay , Kim Dongyoon , Kim Seong‐Min , Yoon Myung‐Han , Stanton Lawrence W. , Je Shawn H. , Yun Seong‐Wook , Chang Young‐Tae 
Published: January 2015

AbstractTo address existing limitations in live neuron imaging, we have developed NeuO, a novel cell‐permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.
Sensitive and Multiplexed On‐chip microRNA Profiling in Oil‐Isolated Hydrogel Chambers
Authors: Lee Hyewon , Srinivas Rathi L. , Gupta Ankur , Doyle Patrick S. 
Published: January 2015

AbstractAlthough microRNAs (miRNAs) have been shown to be excellent indicators of disease state, current profiling platforms are insufficient for clinical translation. Here, we demonstrate a versatile hydrogel‐based microfluidic approach and novel amplification scheme for entirely on‐chip, sensitive, and highly specific miRNA detection without the risk of sequence bias. A simulation‐driven approach is used to engineer the hydrogel geometry and the gel‐reaction environment is chemically optimized for robust detection performance. The assay provides 22.6 fM sensitivity over a three log range, demonstrates multiplexing across at least four targets, and requires just 10.3 ng of total RNA input in a 2 hour and 15 minutes assay.
Reaction of Tungsten–Phosphinidene and –Arsinidene Complexes with Carbodiimides and Alkyl Azides: A Straightforward Way to Four‐Membered Heterocycles
Authors: Seidl Michael , Kuntz Christian , Bodensteiner Michael , Timoshkin Alexey Y. , Scheer Manfred 
Published: January 2015

AbstractThe reaction of the phosphinidene and arsinidene complexes [Cp*E{W(CO)5}2] (E=P (1 a), As (1 b); Cp*=C5Me5) with carbodiimides leads to the new four‐membered heterocycles of the type [Cp*C(NR)2E{W(CO)5}2] (E=P: R=iPr (2 a), Cy (3 a); E=As: R=iPr (2 b), Cy (3 b)). The reaction of phosphinidene complex 1 a with alkyl azides yields the triazaphosphete derivatives [Cp*P{W(CO)5}N(R)NN{W(CO)5}] (R=Hex, Cy) (4). These unprecedented N3P four‐membered triazaphosphete complexes can be regarded as stabilized intermediates of the Staudinger reaction, which have not been previously isolated. All of the isolated products were characterized by NMR, IR spectroscopy, mass spectrometry, and by single‐crystal X‐ray diffraction analysis.
Synthesis and properties of novel trifunctional epoxy triglycidyl of 4‐(4‐aminophenoxy)phenol with high toughness
Authors: Shang Chengyuan , Zhao Xiaojuan , Sun Baogang , Yang Xin , Zhang Ying , Huang Wei 
Published: January 2015

ABSTRACTA trifunctional epoxy containing oxyphenylene unit, triglycidyl of 4‐(4‐aminophenoxy)phenol (TGAPP) was synthesized and characterized. The chemical structure of TGAPP was confirmed with FTIR and 1H‐NMR. DSC analysis revealed that the reactivity of TGAPP with curing agent 4, 4′‐diaminodiphenylsulfone (DDS) was significantly lower than that of triglycidyl para‐aminophenol (TGPAP). Rheological analysis showed that the processing window of TGAPP/DDS was 20°C wider compared with that of TGPAP/DDS. The thermal and mechanical properties of cured TGAPP/DDS were investigated and compared with those of the cured TGPAP/DDS. Experimental results showed that, due to the introduction of oxyphenylene unit, the heat resistance and flexural strength were slightly reduced, while the tensile strength and impact strength were enhanced. SEM also confirmed that the introduction of oxyphenylene unit could enhance the toughness of the TGAPP/DDS as evident from ridge formation. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 41878.
Association of Valine and Leucine at HLA–DRB1 Position 11 With Radiographic Progression in Rheumatoid Arthritis, Independent of the Shared Epitope Alleles but Not Independent of Anti–Citrullinated Protein Antibodies
Authors: van Steenbergen H. W. , Raychaudhuri S. , Rodríguez‐Rodríguez L. , Rantapää‐Dahlqvist S. , Berglin E. , Toes R. E. M. , Huizinga T. W. J. , Fernández‐Gutiérrez B. , Gregersen P. K. , van der Helm‐van Mil A. H. M. 
Published: January 2015

ObjectiveFor decades it has been known that the HLA–DRB1 shared epitope (SE) alleles are associated with an increased risk of development and progression of rheumatoid arthritis (RA). Recently, the following variations in the peptide‐binding grooves of HLA molecules that predispose to RA development have been identified: Val and Leu at HLA–DRB1 position 11, Asp at HLA–B position 9, and Phe at HLA–DPB1 position 9. This study was undertaken to investigate whether these variants are also associated with radiographic progression in RA, independent of SE and anti–citrullinated protein antibody (ACPA) status.MethodsA total of 4,911 radiograph sets from 1,878 RA patients included in the Leiden Early Arthritis Clinic (The Netherlands), Umeå (Sweden), Hospital Clinico San Carlos–Rheumatoid Arthritis (Spain), and National Data Bank for Rheumatic Diseases (US) cohorts were studied. HLA was imputed using single‐nucleotide polymorphism data from an Immunochip, and the amino acids listed above were tested in relation to radiographic progression per cohort using an additive model. Results from the 4 cohorts were combined in inverse‐variance weighted meta‐analyses using a fixed‐effects model. Analyses were conditioned on SE and ACPA status.ResultsVal and Leu at HLA–DRB1 position 11 were associated with more radiographic progression (meta‐analysis P = 5.11 × 10−7); this effect was independent of SE status (meta‐analysis P = 0.022) but not independent of ACPA status. Phe at HLA–DPB1 position 9 was associated with more severe radiographic progression (meta‐analysis P = 0.024), though not independent of SE status. Asp at HLA–B position 9 was not associated with radiographic progression.ConclusionVal and Leu at HLA–DRB1 position 11 conferred a risk of a higher rate of radiographic progression independent of SE status but not independent of ACPA status. These findings support the relevance of these amino acids at position 11.
A robust method for increasing Fc glycan high mannose level of recombinant antibodies
Authors: Huang Chung‐Jr , Lin Henry , Yang Jerry (Xiaoming) 
Published: January 2015

ABSTRACTHigh mannose (HM) glycoforms on antibody Fc glycan are recognized as critical quality attributes for therapeutic antibody products. Methods to control HM have been largely empirical, and it is challenging to target a desired HM level during antibody process development. A novel and robust method to increase antibody HM glycoforms is demonstrated in this study using multiple antibodies and cell lines without adversely impacting cell culture performance, including viable cell density, viability, and protein titer. This approach utilizes mannose as a carbon source and the ratio of mannose to total hexose (glucose and mannose) in feed media determines the extent of HM glycan content of an antibody expressed in cell culture. Scale‐up of this strategy from 3 mL small scale plate to bioreactor (1.5 L) is also demonstrated with comparable results. Further full glycan map analysis shows that HM increase predominantly correlates with the decrease in G0F glycan, with minimum impact on other glycoforms. Possible hypotheses for the HM glycan modulation using mannose as carbon source are also discussed. Three pathways, including GDP‐mannose biosynthesis, early protein glycosylation and UDP‐N‐acetylglucosamine biosynthesis, might be involved and contribute to this HM modulation. Biotechnol. Bioeng. 2015;112: 1200–1209. © 2015 Wiley Periodicals, Inc.
Flavin‐Functionalized Gold Nanoparticles as an Efficient Catalyst for Aerobic Organic Transformations
Authors: Imada Yasushi , Ohsaki Motonari , Noguchi Mikiko , Maeda Takatoshi , Fujiki Misa , Kawamorita Soichiro , Komiya Naruyoshi , Naota Takeshi 
Published: January 2015

AbstractThe front cover artwork for Issue 1/2015 is provided by the research group of Synthetic Organic Chemistry, Graduate School of Engineering Science, Osaka University (Japan). The image shows the catalytic activities of puffball‐shaped nanoparticles for environmentally benign aerobic oxidation and reduction. See the Full Paper itself at http://dx.doi.org/10.1002/cctc.20140916.
The Gold(I)‐Catalysed Protodecarboxylation Mechanism
Authors: Dupuy Stéphanie , Crawford L. Ellis , Bühl Michael , Nolan Steven P. 
Published: January 2015

AbstractA mechanistic study of the gold‐catalysed protodecarboxylation is described. Each reaction step has been investigated experimentally and computationally. More specifically, the activation parameters for the decarboxylation step have been determined through kinetic studies. Further experimental studies on the hydrolysis of the arylgold intermediate have revealed that the protodeauration can become competitive with the decarboxylation process at high conversions. This switch in rate‐limiting step has been shown to be pKa‐dependent. These studies have been supported by DFT calculations and permit a better understanding of which prevalent features of the reaction mechanism account for the decarboxylation process.
Luminescent Ag6Au6 Heterometallic Ethisterone Cluster and Probe for Estrogen Receptor α
Authors: Guo Wen‐Jing , Wang Yong‐Tao , Kong De‐Xian , Wang Jin‐Yun , Wei Qiao‐Hua , Chen Guo‐Nan 
Published: January 2015

AbstractA heterometallic cluster [Ag6Au6(ethisterone)12] of an unprecedented topology was synthesized and characterized. A sensitive and specific probe for estrogen receptor α (ERα) has been developed for the first time based on the enhancement of the Ag6Au6 luminescence.
Nanomolar Detection of Iodide in Aqueous Medium Using Organic–Inorganic Hybrid Nanoparticles: Application in Urine Analysis
Authors: Huerta Aguilar Carlos Alberto , Sharma Hemant , Thangarasu Pandiyan , Singh Narinder 
Published: January 2015

AbstractImine‐linked pyridine‐coupled (ILPC) receptors have been synthesised and characterised. The absorption and fluorescence properties of these receptors have been explored through a combination of experimental and theoretical studies. The ILPC receptors are processed into organic nanoparticles (ONPs) and then decorated with gold nanoparticles (AuNPs) for the selective recognition of iodide. The selective recognition behaviour is authenticated with the changes in fluorescence spectra, low detection limit (1.4 nM) and no interference in aqueous systems. The present investigation represents the first example of nanomolar detection of iodide in aqueous medium using organic–inorganic hybrid nanoparticles (ONPs–AuNPs). The probe has been utilised successfully for the detection of iodide content in urine samples.
Across the Board: Hiroshi Imahori
Authors: Imahori Hiroshi 
Published: January 2015

AbstractIn this series of articles the board members of ChemSusChem discuss recent research articles that they consider of exceptional quality and importance for sustainability. This entry features Prof. Hiroshi Imahori, who discusses a concept in which photosensitizers and binuclear catalysts, both based on ruthenium complexes, can be combined to efficiently oxidize water similar to the Photosystem II in plants.
Thin Layer Coulometry of Nitrite with Ion‐Selective Membranes
Authors: Afshar Majid Ghahraman , Crespo Gastón A. , Dorokhin Denis , Néel Bastien , Bakker Eric 
Published: January 2015

AbstractWe report on a thin layer coulometric detection method based on an ion‐selective membrane in view of a quantitative determination of nitrite in undiluted human urine and tap water. A cobalt(II) tert‐butyl salophen compound, previously characterized by our group in ion‐selective membrane electrodes, is used here as ionophore. The four orders of magnitude discrimination of chloride permits the detection of nitrite in samples that contain a large excess of chloride. The approach developed here allows one to determine nitrite within a concentration range of 20–100 µM for a 120 s current integration time. A modification of the reported electrochemical protocol to reduce the capacitive contribution diminishes the intercept of the calibration to −8.6 µC. This is especially important in view of reaching calibration free systems. Initial in‐house validation exhibits a limit of detection of 10 µM in undiluted urine and in tap water. These values correspond to the regulation limits established in European Union and are adequate for the determination of nitrite for medical applications.
The cardiac dose‐sparing benefits of deep inspiration breath‐hold in left breast irradiation: a systematic review
Authors: Smyth Lloyd M. , Knight Kellie A. , Aarons Yolanda K. , Wasiak Jason 
Published: January 2015

AbstractIntroductionDespite technical advancements in breast radiation therapy, cardiac structures are still subject to significant levels of irradiation. As the use of adjuvant radiation therapy after breast‐conserving surgery continues to improve survival for early breast cancer patients, the associated radiation‐induced cardiac toxicities become increasingly relevant. Our primary aim was to evaluate the cardiac‐sparing benefits of the deep inspiration breath‐hold (DIBH) technique.MethodsAn electronic literature search of the PubMed database from 1966 to July 2014 was used to identify articles published in English relating to the dosimetric benefits of DIBH. Studies comparing the mean heart dose of DIBH and free breathing treatment plans for left breast cancer patients were eligible to be included in the review. Studies evaluating the reproducibility and stability of the DIBH technique were also reviewed.ResultsTen studies provided data on the benefits of DIBH during left breast irradiation. From these studies, DIBH reduced the mean heart dose by up to 3.4 Gy when compared to a free breathing approach. Four studies reported that the DIBH technique was stable and reproducible on a daily basis. According to current estimates of the excess cardiac toxicity associated with radiation therapy, a 3.4 Gy reduction in mean heart dose is equivalent to a 13.6% reduction in the projected increase in risk of heart disease.ConclusionDIBH is a reproducible and stable technique for left breast irradiation showing significant promise in reducing the late cardiac toxicities associated with radiation therapy.
Improving the catalytic behavior of inulin fructotransferase under high hydrostatic pressure
Authors: Li Yungao , Miao Ming , Chen Xiangyin , Jiang Bo , Liu Miao , Feng Biao 
Published: January 2015

AbstractBACKGROUNDThe demand for difructose anhydride III (DFA III), a novel functional sweetener, is growing continuously. It is produced from inulin by inulin fructotransferase (IFTase). In this study, high hydrostatic pressure (HHP), as a clean technology, was first applied to further improve the catalytic efficiency of IFTase in the process.RESULTSThe maximum activity of IFTase was obtained under 200 MPa at 60 °C. Meanwhile, HHP lowered the energy barrier necessary for the enzymatic reaction and decreased the volume between the reactants and the transition state. Under this condition, the optimal pH for the enzymatic reaction shifted from 5.5 to 6.0. The activity was further enhanced by 65.2% in the presence of 1.5 mol L−1 NaCl.CONCLUSIONThe catalytic reaction of IFTase was performed under HHP for the first time. HHP, as a promising green technology for bioconversion, significantly accelerated the enzymatic reaction under the appropriate operational conditions. © 2015 Society of Chemical Industry
Robust control and fault detection for continuous‐time switched systems subject to a dwell time constraint
Authors: Zhong Guang‐Xin , Yang Guang‐Hong 
Published: January 2015

SummaryThis paper is concerned with the simultaneous robust control and fault detection problem for continuous‐time switched systems subject to a dwell time constraint. To meet the control and detection objectives under the constraint, the controller/detectors matching different time intervals are first constructed in an output feedback framework. A state‐dependent switching law that obeys the dwell time constraint is then designed such that the closed‐loop switched system is asymptotically stable and also with the robust and detection performance. Further, the proposed switching law is dependent only on the partial measurable states of the closed‐loop system, which is applicable when the states of system mode are fully unavailable. Thus, our result extends the existing ones in state‐dependent switching and state‐feedback frameworks. Finally, a numerical example is given to illustrate the effectiveness of the proposed method. Copyright © 2015 John Wiley & Sons, Ltd.
Bayesian visual analytics: BaVA
Authors: House Leanna , Leman Scotland , Han Chao 
Published: January 2015

AbstractLeman et al. and Endert et al. develop an interactive data visualization framework called visual to parametric interaction (V2PI). With V2PI, experts may explore data visually (assess multiple data visualizations) based on their judgments and an underlying data analytic method. Specifically, V2PI offers a deterministic procedure to quantify expert judgments and update analytical parameters to create new data visualizations. In this article, we explain V2PI from a probabilistic perspective and develop Bayesian visual analytics (BaVA). We model data probabilistically, develop parallels between quantifying expert judgments and eliciting prior distributions from experts, and justify how we update parameters using Bayesian sequential updating. We apply BaVA using two linear projections methods to assess simulated and real‐world datasets.
Laws of mission-based programming
Authors: Zeyda Frank , Cavalcanti Ana 
Published: January 2015

Abstract

Safety-Critical Java (SCJ) is a recent technology that changes the execution and memory model of Java in such a way that applications can be statically analysed and certified for their real-time properties and safe use of memory. Our interest is in the development of comprehensive and sound techniques for the formal specification, refinement, design, and implementation of SCJ programs, using a correct-by-construction approach. As part of this work, we present here an account of laws and patterns that are of general use for the refinement of SCJ mission specifications into designs of parallel handlers, as they are used in the SCJ programming paradigm. Our refinement notation is a combination of languages from the

Circus

family, supporting state-rich reactive models with the addition of class objects and real-time properties. Starting from a sequential and centralised

Circus

specification, our laws permit refinement into

Circus

models of SCJ program designs. Automation and proof of the refinement laws is examined here, too. Our work is an important step towards eliciting laws of programming for SCJ and fits into a refinement strategy that we have developed previously to derive SCJ programs from specifications in a rigorous manner.
Model checking dynamic pushdown networks
Authors: Song Fu , Touili Tayssir 
Published: January 2015

Abstract

A dynamic pushdown network (DPN) is a set of pushdown systems (PDSs) where each process can dynamically create new instances of PDSs. DPNs are a natural model of multi-threaded programs with (possibly recursive) procedure calls and thread creation. Thus, it is important to have model checking algorithms for DPNs. We consider in this work model checking DPNs against single-indexed LTL and CTL properties of the form

⋀

f
i

such that
f

i

is a LTL/CTL formula over the PDS
i
. We consider the model checking problems w.r.t. simple valuations (i.e., whether a configuration satisfies an atomic proposition depends only on its control location) and w.r.t. regular valuations (i.e., the set of the configurations satisfying an atomic proposition is a regular set of configurations). We show that these model checking problems are decidable. We propose automata-based approaches for computing the set of configurations of a DPN that satisfy the corresponding single-indexed LTL/CTL formula.
Special Section on LGBT Resilience Across Cultures: Introduction
Authors: Beasley Christopher R. , Jenkins Richard A. , Valenti Maria 
Published: January 2015

AbstractThis special section addresses a gap area of resilience and LGBT well‐being. Although comprehensive global diversity regarding LGBT resilience was challenging to find, the special section includes representation from outside the US (Israel and Hong Kong), ethnic/racially diverse domestic populations, immigration, and one population for which LGBT identities might be considered marginalized—Christians in the US. The full range of LGBT identities are represented in the issue along with persons identifying as queer or questioning, although transgendered people were less well represented than lesbian, gay or bisexual identities.
Examination of placental three‐dimensional power Doppler indices and perinatal outcome in pregnancies complicated by intrauterine growth restriction
Authors: Molnár András , Surányi Andrea , Nyári Tibor , Németh Gábor , Pál Attila 
Published: January 2015

AbstractObjectiveTo examine placental vascularization using three‐dimensional power Doppler (3DPD) ultrasonography in pregnancies complicated by intrauterine growth restriction (IUGR).MethodsThe present prospective study was conducted at the University of Szeged (Szeged, Hungary) from February 2012 to March 2013. Women with a singleton pregnancy who attended the maternity outpatient ward in the second or third trimester were enrolled consecutively. Women were divided into two groups: those with a normal pregnancy and those with a pregnancy complicated by IUGR. Three 3DPD indices—vascularization index (VI), flow index (FI), and vascularization flow index (VFI)—were assessed.ResultsA total of 223 women were enrolled: 171 were in the control group and 52 in the IUGR group. Median VI was 3.7% (interquartile range [IQR] 3.2%–4.2%) in the IUGR group and 10.1% (IQR 8.6%–10.9%) in the control group (P = 0.001). Median FI was 40.0 (IQR 39.7–42.5) in the IUGR group and 45.1 (IQR 44.1–53.1) in the control group (P = 0.012). Median VFI was 2.2 (IQR 2.1–2.4) in the IUGR group and 4.8 (IQR 4.4–5.3) in the control group (P < 0.001).ConclusionPlacental vascularization was reduced in pregnancies complicated by IUGR.
On condition based maintenance policy
Authors: Shin Jong-Ho , Jun Hong-Bae 
Published: January 2015

Abstract
In the case of a high-valuable asset, the Operation and Maintenance (O&M) phase requires heavy charges and more efforts than the installation (construction) phase, because it has long usage life and any accident of an asset during this period causes catastrophic damage to an industry. Recently, with the advent of emerging Information Communication Technologies (ICTs), we can get the visibility of asset status information during its usage period. It gives us new challenging issues for improving the efficiency of asset operations. One issue is to implement the Condition-Based Maintenance (CBM) approach that makes a diagnosis of the asset status based on wire or wireless monitored data, predicts the assets abnormality, and executes suitable maintenance actions such as repair and replacement before serious problems happen. In this study, we have addressed several aspects of CBM approach: definition, related international standards, procedure, and techniques with the introduction of some relevant case studies that we have carried out.