bluuebunny/crossref_metadata_2025_split huggingface dataset:
Salvage of Avascular Necrosis of the Talus by Combined Ankle and Hindfoot Arthrodesis Without Structural Bone Graft
Authors: Tenenbaum Shay , Stockton Kristopher G. , Bariteau Jason T. , Brodsky James W. 
Published: November 2014

Background: Osteonecrosis of the talus is a well-recognized pathology, which can result in significant hindfoot collapse resulting in poor function and pain. Treatment with intramedullary tibiotalocalcaneal arthrodesis (IMTCA) using a retrograde intramedullary nail is widely utilized for severe concomitant tibiotalar and subtalar pathologies. This study reports the results of ankle and hindfoot arthrodesis in patients with arthritis and deformity caused specifically by talar osteonecrosis. Methods: Fourteen ankle and hindfoot arthrodeses with retrograde intramedullary nail were studied, with a mean follow-up of 26 months. Medical records were reviewed for operative technique, concomitant procedures, bone graft used, and postoperative complications including nonunion, infection, nerve injury, wound healing issues, and the need for additional surgeries. Clinical outcomes included Visual Analogue Scale for pain, the AOFAS Ankle/Hindfoot Score, and the SF-36 questionnaire. Results: Over 80% of cases had osteonecrosis involving the entire body of the talus. In 4 cases tibiocalcaneal arthrodesis was performed, with the remaining talar head-neck portion fused to anterior aspect of tibia. Union was achieved in all cases. The mean preoperative VAS score was 6.9 (range 5 to 9, SD ± 1.5) decreasing to 1.7 (range 0 to 6, SD ± 2.2) postoperatively ( P = .00008). The mean preoperative AOFAS score was 32.7 (range 20 to 46, SD ± 8.7), increasing to 72.1 (range 46 to 86, SD ± 10.1, P = .00003). The mean preoperative SF-36 physical component score was 30.5 (range 21 to 42, SD ± 6.9) increasing to 42.8 (range 20 to 60, SD ± 11.4) postoperatively ( P = .02). Complications included 1 stress fracture, 4 hardware removals, and 1 superficial infection. Conclusion: Ankle and hindfoot arthrosis due to extensive talar AVN can be successfully treated with IMTCA. Level of Evidence: Level IV, retrospective case series.
Hyperthermic intraperitoneal chemotherapy with oxaliplatin as treatment for peritoneal carcinomatosis arising from the appendix and pseudomyxoma peritonei: a survival analysis
Authors: Marcotte Eric , Dubé Pierre , Drolet Pierre , Mitchell Andrew , Frenette Suzanne , Leblanc Guy , Leclerc Yves E , Sideris Lucas 
Published: November 2014

Abstract

Background
Appendiceal peritoneal carcinomatosis (PC) is rare and its long-term prognosis is poor. The aim of this study was to evaluate the results of an aggressive treatment approach used in our institution for the last eight years.

Methods
Data from all patients with PC arising from the appendix were prospectively collected and analyzed. Treatment consisted of complete surgical cytoreduction (CRS), followed by hyperthermic intraperitoneal chemotherapy (HIPEC) with oxaliplatin (460 mg/m2) at 43°C over 30 minutes. Ronnett’s histologic classification was used for tumor grading.

Results
Between February 2003 and April 2011, 78 patients underwent laparotomy with curative intent. The mean follow-up period was 33.7 months. A total of 58 patients received HIPEC, but 11 patients could not have CRS and received no HIPEC. Nine patients with a negative second-look surgery also received no HIPEC. The five-year overall survival for the entire cohort was 66.2%; 100% for the negative second-look patients, 77% for the HIPEC patients and 9% for the unresectable patients (P <0.0001). A total of 15 patients (25.9%) had isolated peritoneal recurrence, no patient had visceral recurrence only, and five patients (8.6%) had both. In regards to the five-year disease-free survival for the HIPEC patients, histologic grade (disseminated peritoneal adenomucinosis 100%, peritoneal mucinous carcinomatosis with intermediate features 40%, peritoneal mucinous carcinomatosis 20%; p =0.0016) and completeness of cytoreduction (CCR-0 56%, CCR-1 24%; P =0.0172) were prognostic factors. There was one postoperative mortality. The major complication rate for patients treated with HIPEC was 40%, including intra-abdominal abcess (17%), hemorrhage (12%) and anastomotic leak (10%). One patient in the HIPEC group experienced temporary grade II neuropathy and grade III thrombocytopenia.

Conclusions
This therapeutic approach seems both feasible and safe in selected patients. Recurrence is, however, frequent and represents a challenge.
Profiling gene promoter occupancy of Sox2 in two phenotypically distinct breast cancer cell subsets using chromatin immunoprecipitation and genome-wide promoter microarrays
Authors: Jung Karen , Wang Peng , Gupta Nidhi , Gopal Keshav , Wu Fang , Ye Xiaoxia , Alshareef Abdulraheem , Bigras Gilbert , McMullen Todd P , Abdulkarim Bassam S , Lai Raymond 
Published: November 2014

Abstract
Introduction
Aberrant expression of the embryonic stem cell marker Sox2 has been reported in breast cancer (BC). We previously identified two phenotypically distinct BC cell subsets separated based on their differential response to a Sox2 transcription activity reporter, namely the reporter-unresponsive (RU) and the more tumorigenic reporter-responsive (RR) cells. We hypothesized that Sox2, as a transcription factor, contributes to their phenotypic differences by mediating differential gene expression in these two cell subsets.

Methods
We used chromatin immunoprecipitation and a human genome-wide promoter microarray (ChIP-chip) to determine the promoter occupancies of Sox2 in the MCF7 RU and RR breast cancer cell populations. We validated our findings with conventional chromatin immunoprecipitation, quantitative reverse transcription polymerase chain reaction (qPCR), and western blotting using cell lines, and also performed qPCR using patient RU and RR samples.

Results
We found a largely mutually exclusive profile of gene promoters bound by Sox2 between RU and RR cells derived from MCF7 (1830 and 456 genes, respectively, with only 62 overlapping genes). Sox2 was bound to stem cell- and cancer-associated genes in RR cells. Using quantitative RT-PCR, we confirmed that 15 such genes, including PROM1 (CD133), BMI1, GPR49 (LGR5), and MUC15, were expressed significantly higher in RR cells. Using siRNA knockdown or enforced expression of Sox2, we found that Sox2 directly contributes to the higher expression of these genes in RR cells. Mucin-15, a novel Sox2 downstream target in BC, contributes to the mammosphere formation of BC cells. Parallel findings were observed in the RU and RR cells derived from patient samples.

Conclusions
In conclusion, our data supports the model that the Sox2 induces differential gene expression in the two distinct cell subsets in BC, and contributes to their phenotypic differences.
Whole genome sequencing of SIV-infected macaques identifies candidate loci that may contribute to host control of virus replication
Authors: Ericsen Adam J , Starrett Gabriel J , Greene Justin M , Lauck Michael , Raveendran Muthuswamy , Deiros David Rio , Mohns Mariel S , Vince Nicolas , Cain Brian T , Pham Ngoc H , Weinfurter Jason T , Bailey Adam L , Budde Melisa L , Wiseman Roger W , Gibbs Richard , Muzny Donna , Friedrich Thomas C , Rogers Jeffrey , O’Connor David H 
Published: November 2014

Abstract
Background
A small percentage of human immunodeficiency virus (HIV)-infected people and simian immunodeficiency virus (SIV)-infected macaques control virus replication without antiretroviral treatment. The major determinant of this control is host expression of certain major histocompatibility complex alleles. However, this association is incompletely penetrant, suggesting that additional loci modify the major histocompatibility complex's protective effect. Here, to identify candidate control-modifying loci, we sequence the genomes of 12 SIV-infected Mauritian cynomolgus macaques that experienced divergent viral load set points despite sharing the protective M1 major histocompatibility complex haplotype.

Results
Our genome-wide analysis of haplotype-level variation identifies seven candidate control-modifying loci on chromosomes 2, 3, 7, 8, 9, 10, and 14. The highest variant density marks the candidate on chromosome 7, which is the only control-modifying locus to comprise genes with known immunological function. Upon closer inspection, we found an allele for one of these genes, granzyme B, to be enriched in M1(+) controllers. Given its established role as a cytotoxic effector molecule that participates in CD8-mediated killing of virus-infected cells, we test the role of variation within gzmb in modifying SIV control by prospectively challenging M1(+) granzyme B-defined macaques.

Conclusions
Our study establishes a framework for using whole genome sequencing to identify haplotypes that may contribute to complex clinical phenotypes. Further investigation into the immunogenetics underlying spontaneous HIV control may contribute to the rational design of a vaccine that prevents acquired immune deficiency syndrome.
Bringing non-human primate research into the post-genomic era: how monkeys are teaching us about elite controllers of HIV/AIDS
Authors: Vallender Eric J 
Published: November 2014

AbstractWhole-genome sequencing of Mauritian cynomolgus macaques reveals novel candidate loci for controlling simian immunodeficiency virus replication.See related Research, http://genomebiology.com/2014/15/11/478
Quantum reconstruction for Fano bundles on projective space
Authors: Strangeway Andrew 
Published: November 2014

AbstractWe present a reconstruction theorem for Fano vector bundles on projective space which recovers the small quantum cohomology for the projectivization of the bundle from a small number of low-degree Gromov-Witten invariants. We provide an extended example in which we calculate the quantum cohomology of a certain Fano 9-fold and deduce from this, using the quantum Lefschetz theorem, the quantum period sequence for a Fano 3-fold of Picard rank 2 and degree 24. This example is new, and is important for the Fanosearch program.
An Efficient Synthesis of Aurone Derivatives by the Tributylphosphine-catalyzed Regioselective Cyclization of o-Alkynoylphenols
Authors: Saito Koya , Yoshida Masahito , Doi Takayuki 
Published: November 2014

Abstract
An organocatalytic regioselective synthesis of aurones from o-alkynoylphenols was achieved. A catalytic amount of tributylphosphine selectively induced the 5-exo cyclization of o-alkynoylphenols under ambient conditions leading to aurone derivatives in high to excellent yields.
Selective Polymerization of Dimethylsilylphenylacetylene and the Gas Permselectivity of the Resulting Polymer Membranes
Authors: Li Jun , Wang Jianjun , Aoki Toshiki , Kaneko Takashi , Teraguchi Masahiro 
Published: November 2014

Abstract
Three types of homopolymerizations of p-(dimethylsilyl)phenylacetylene (HSPA) having a terminal triple bond and a Si–H group were selectively carried out by using three kinds of polymerization catalysts. Among them poly(HSPA) synthesized by addition polymerization of its triple bond using [Rh(nbd)Cl]2 (nbd: norbornadiene)/phenylethylamine (PEA) showed a good membrane forming ability. The copolymerizations of HSPA with p-(trimethylsilyl)phenylacetylene (SPA) were carried out using [Rh(nbd)Cl]2/PEA and the resulting copolymers were fabricated to self-supporting membranes. These membranes, including the homopolymer of HSPA, showed higher permselectivities for oxygen and carbon dioxide over nitrogen than those of poly(SPA). Incorporation of HSPA unit to poly(SPA) effectively enhanced its permselectivities with only small decrease in its permeability.
Femoral neck fractures
Authors: Su E. P. , Su S. L. 
Published: November 2014

Surgical interventions consisting of internal fixation (IF) or total hip replacement (THR) are required to restore patient mobility after hip fractures. Conventionally, this decision was based solely upon the degree of fracture displacement. However, in the last ten years, there has been a move to incorporate patient characteristics into the decision making process. Research demonstrating that joint replacement renders superior functional results when compared with IF, in the treatment of displaced femoral neck fractures, has swayed the pendulum in favour of THR. However, a high risk of dislocation has always been the concern. Fortunately, there are newer technologies and alternative surgical approaches that can help reduce the risk of dislocation. The authors propose an algorithm for the treatment of femoral neck fractures: if minimally displaced, in the absence of hip joint arthritis, IF should be performed; if arthritis is present, or the fracture is displaced, then THR is preferred.Cite this article: Bone Joint J 2014;96-B(11 Suppl A):43–7.
The multiply-operated total knee replacement patient
Authors: Haidukewych G. J. , Petrie J. R. , Adigweme O. 
Published: November 2014

Although the vast majority of patients that undergo total knee replacement have satisfactory outcomes with a generally low complication rate, occasionally a patient will be encountered that has had multiple failed surgeries, and now reaches a crossroad as to whether limb salvage will be acceptable or not. Cite this article: Bone Joint J 2014;96-B(11 Suppl A):122–4.
The revision acetabulum – allograft and bone substitutes
Authors: Callaghan J. J. , Liu S. S. , Phruetthiphat O-A. 
Published: November 2014

A common situation presenting to the orthopaedic surgeon today is a worn acetabular liner with substantial acetabular and pelvic osteolysis. The surgeon has many options for dealing with osteolytic defects. These include allograft, calcium based substitutes, demineralised bone matrix, or combinations of these options with or without addition of platelet rich plasma. To date there are no clinical studies to determine the efficacy of using bone-stimulating materials in osteolytic defects at the time of revision surgery and there are surprisingly few studies demonstrating the clinical efficacy of these treatment options. Even when radiographs appear to demonstrate incorporation of graft material CT studies have shown that incorporation is incomplete. The surgeon, in choosing a graft material for a surgical procedure must take into account the efficacy, safety, cost and convenience of that material. Cite this article: Bone Joint J 2014;96-B (11 Suppl A):70–2.
Die digitale Transformation eines ganzen Jahrhunderts: Digitalisierung der Zeitungen des 17. Jahrhunderts an der SuUB Bremen
Authors: Müller Maria Elisabeth , Hermes Maria 
Published: November 2014

Zusammenfassung:
Seit Mai 2013 fördert die Deutsche Forschungsgemeinschaft (DFG) Pilotprojekte zur Digitalisierung historischer Zeitungen in vier Bibliotheken Deutschlands, u. a. in der Staats- und Universitätsbibliothek Bremen. Zudem erfolgen der Ausbau der Zeitschriftendatenbank (ZDB) und die Anpassung des DFG-Viewers für Zeitungen. Neben der signifikanten Vermehrung digitalisierter Zeitungen erarbeiten die am Rahmenprojekt teilnehmenden Bibliotheken Standards der Zeitungsdigitalisierung, die auch für zukünftige Digitalisierungsprojekte Relevanz haben.
Das Pilotprojekt der SuUB Bremen hat sich zum Ziel gesetzt, die kompletten deutschsprachigen Zeitungen des 17. Jahrhunderts zu digitalisieren, die in keiner anderen Bibliothek so vollständig vorhanden sind. Die Präsentation der Zeitungen erfolgt im Portal Digitale Sammlungen der SuUB Bremen (http://brema.suub.uni-bremen.de/) auf der Basis der Software Visual Library. Der Beitrag stellt die Zeitungsdigitalisierung im Kontext der aktuellen Förderpolitik der DFG vor. Neben der Herausstellung der standard- und strukturbildenden Ziele der Pilotprojekte werden die spezifische Ausrichtung und die Besonderheiten des Bremer Projekts thematisiert.
The effects of asymmetrical loading on gait characteristics
Authors: Shi Ping , Fang Youfang , Guo Mingming , Yu Hongliu 
Published: November 2014

AbstractAsymmetrical load carriage is a popular method used among people in daily life. This study investigated the impact of asymmetrical load carriage and the load mass spatiotemporal parameters associated with gait patterns. Twelve participants were recorded as they walked under six load conditions. The loads included a control condition in which individuals carried no added load and loads of 10%, 15%, 20%, 25%, and 30% body weight carried with their right hand. Parameters used to describe the spatiotemporal characteristics of gait were quantified and analyzed. The results of the study demonstrated that the gait patterns of the individuals were affected by increases in the carried load. The loaded right side was more sensitive to changes in the loads carried. Overall, we conclude that the spatiotemporal parameters actually depend on the combination of both loading mass and the side that was loaded.
Different methods to estimate serum free cortisol: a comparison during cortisol tetracosactide testing
Authors: Brossaud Julie , Gatta Blandine , Tabarin Antoine , Corcuff Jean-Benoît 
Published: November 2014

AbstractSerum cortisol is routinely quantified by immunoassays. In intensive care units serum free cortisol (FC) determination has been described as a better indicator of survival than total cortisol (TC). To estimate FC different methods are available including saliva sampling. We compared five methods to estimate FC, before and after an ACTH stimulating test in patients suspected of adrenal insufficiency.Serum and saliva was collected from 130 patients from the Endocrine Department of a university hospital before and after tetracosactide injection for TC determination. FC was estimated: after serum ultrafiltration, quadratic (Coolens’) or cubic (Dorin’s) equations, using TC/cortisol-binding globulin concentrations ratio or using cortisol concentration determination in saliva.FC concentrations obtained by different techniques were significantly correlated and Passing-Bablok regressions showed no deviation from linearity between salFC and filtFC or quadFC. Using the routine assumption that the patients were correctly diagnosed using a post-tetracosactide TC threshold of 550 nmol/L the FC methods generating the best ROC curves wereFC concentrations obtained by different techniques are significantly but not similarly correlated with TC. As,
Finite Element Simulation of Weld Bead Geometry and Temperature Distribution during GTA Welding of Modified 9Cr-1Mo Steel and Experimental Validation
Authors: Zubairuddin M. , Albert S.K. , Vasudevan M. , Chaudhari V. , Suri V.K. 
Published: November 2014

AbstractThe thermal analysis of Modified 9Cr-1Mo steel plate during GTA welding is carried out using finite element method employing SYSWELD software. First, the simulation of bead on plate welds for 16 varying process parameter conditions by employing a double ellipsoidal heat source was carried out. Simulated bead on plate weld bead dimensions for the 16 welds were compared with the actual bead dimensions obtained experimentally. Then the simulation of GTA welding of square butt joint on 3 mm thick plates of dimensions 300 × 125 × 3 mm was carried out. The simulated thermal profiles were validated using thermocouple measurements. There was good agreement between the predicted and measured weld bead profiles and thermal cycles for square butt joint. The investigation on the effect of copper back up plate on the peak temperature and the cooling rate has revealed that the peak temperature decreased by 147 °C and the cooling rate increased by 35%.
The mammalian tRNA ligase complex mediates splicing of XBP1 mRNA and controls antibody secretion in plasma cells
Authors: Jurkin Jennifer , Henkel Theresa , Nielsen Anne Færch , Minnich Martina , Popow Johannes , Kaufmann Therese , Heindl Katrin , Hoffmann Thomas , Busslinger Meinrad , Martinez Javier 
Published: November 2014

AbstractThe unfolded protein response (UPR) is a conserved stress‐signaling pathway activated after accumulation of unfolded proteins within the endoplasmic reticulum (ER). Active UPR signaling leads to unconventional, enzymatic splicing of XBP1 mRNA enabling expression of the transcription factor XBP1s to control ER homeostasis. While IRE1 has been identified as the endoribonuclease required for cleavage of this mRNA, the corresponding ligase in mammalian cells has remained elusive. Here, we report that RTCB, the catalytic subunit of the tRNA ligase complex, and its co‐factor archease mediate XBP1 mRNA splicing both in vitro and in vivo. Depletion of RTCB in plasma cells of Rtcbfl/fl Cd23‐Cre mice prevents XBP1s expression, which normally is strongly induced during plasma cell development. RTCB‐depleted plasma cells show reduced and disorganized ER structures as well as severe defects in antibody secretion. Targeting RTCB and/or archease thus represents a promising strategy for the treatment of a growing number of diseases associated with elevated expression of XBP1s.
Foxd3 suppresses NFAT‐mediated differentiation to maintain self‐renewal of embryonic stem cells
Authors: Zhu Lili , Zhang Shiyue , Jin Ying 
Published: November 2014

AbstractPluripotency‐associated transcription factor Foxd3 is required for maintaining pluripotent cells. However, molecular mechanisms underlying its function are largely unknown. Here, we report that Foxd3 suppresses differentiation induced by calcineurin–NFAT signaling to maintain the ESC identity. Mechanistically, Foxd3 interacts with NFAT proteins and recruits co‐repressor Tle4, a member of the Tle repressor family highly expressed in undifferentiated ESCs, to suppress NFATc3's transcriptional activities. Furthermore, global transcriptome analysis shows that Foxd3 and NFATc3 co‐regulate a set of differentiation‐associated genes in ESCs. Collectively, our study establishes a molecular and functional link between a pluripotency‐associated factor and an important ESC differentiation‐inducing pathway.
BMI is an important driver of β-cell loss in type 1 diabetes upon diagnosis in 10 to 18-year-old children
Authors: Lauria A , Barker A , Schloot N , Hosszufalusi N , Ludvigsson J , Mathieu C , Mauricio D , Nordwall M , Van der Schueren B , Mandrup-Poulsen T , A Scherbaum W , Weets I , K Gorus F , Wareham N , D Leslie R , Pozzilli P 
Published: November 2014

ObjectiveBody weight-related insulin resistance probably plays a role in progression to type 1 diabetes, but has an uncertain impact following diagnosis. In this study, we investigated whether BMI measured at diagnosis was an independent predictor of C-peptide decline 1-year post-diagnosis.DesignMulticentre longitudinal study carried out at diagnosis and up to 1-year follow-up.MethodsData on C-peptide were collected from seven diabetes centres in Europe. Patients were grouped according to age at diagnosis (<5 years, n=126; >5 years <10 years, n=295; >10 years <18 years, n=421; >18 years, n=410). Linear regression was used to investigate whether BMI was an independent predictor of change in fasting C-peptide over 1 year. Models were additionally adjusted for baseline insulin dose and HbA1c.ResultsIn individuals diagnosed between 0 and 5 years, 5 and 10 years and those diagnosed >18 years, we found no association between BMI and C-peptide decline. In patients aged 10–18 years, higher BMI at baseline was associated with a greater decline in fasting C-peptide over 1 year with a decrease (β 95% CI; P value) of 0.025 (0.010, 0.041) nM/kg per m2 higher baseline BMI (P=0.001). This association remained significant after adjusting for gender and differences in HbA1c and insulin dose (β=0.026, 95% CI=0.0097, 0.042; P=0.002).ConclusionsThese observations indicate that increased body weight and increased insulin demand are associated with more rapid disease progression after diagnosis of type 1 diabetes in an age group 10–18 years. This should be considered in studies of β-cell function in type 1 diabetes.