bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and validation of an instrument to evaluate Person-Centered care in health services
Authors: Lehmann-Mendoza Rodolfo , Cortés-Moreno Gabriela Yanet , Sarabia-González Odet , Figuerola-Escoto Rosa Paola , Luna David , González-Mundo Ilicia , Caselín-Ledezma Rubén , Vázquez-Dávila Roberto Arturo , Martínez-Arredondo Hilario Ascención 
Published: August 2024

Abstract
Background
The concept of quality in health care has evolved, placing greater importance on the patient’s needs, culture, and social context, as well as their participation in clinical decision-making, as highlighted by Mead and Bower’s Person-Centered Care Model. The aim of the present study was to design and validate an instrument to assess the extent to which healthcare services provided by PEMEX (Petróleos Mexicanos) offer person-centered care according to user perceptions.

Methods
The first phase comprised the development of 57 items based on the analysis of responses from an open-ended questionnaire administered to 30 users of Pemex healthcare services. This questionnaire was designed considering the four factors of the person-centered care model, however, the high correlation between the 4 factors (i.e., r ≥ .80) indicated an overfactoring effect and consequently an increase in the risk of overfitting. Therefore, an exhaustive analysis of the instrument was performed, starting with the review of the individual behavior of each item, and carrying out exploratory and confirmatory factor analysis. Using a sample of 330 individuals, an exploratory factor analysis was perfomed. Afterward, a confirmatory factorial analysis was carried out with 335 participants. Finally, a new confirmatory factorial analysis included 130 participants due to the refinements made in the previous phase. Internal consistency was assessed using Cronbach’s α and McDonald’s ω at every phase.

Results
The exploratory factor analysis retained 35 items in a single factor that accounted for 49% of the variance with an internal consistency of Cronbach’s α and McDonald’s ω = 0.97. Because the factorial structure by confirmatory factorial analysis was unsatisfactory, the initial model was refined, leading to the retention of 11 items and a final model with adjustment index of χ2 = 127.53, χ2/gl = 2.89, RMSEA = 0.07, IC RMSEA 0.06 to 0.09, TLI = 0.95 and CFI = 0.96, with an internal consistency of Cronbach’s α and McDonald’s ω = 0.93. Due to the refinements, a new confirmatory factorial analysis was conducted with suitable goodness-of-fit criteria in most items (χ2 = 151.44, χ2/gl = 3.43, RMSEA = 0.13, IC RMSEA 0.11 to 0.16, TLI = 0.93 and CFI = 0.94), resulting in a Cronbach’s α and McDonald’s ω = 0.98.

Conclusions
The instrument exhibits suitable psychometric properties to be employed to measure the degree to which medical care is patient centered. This instrument represents a strategy for promoting an innovative healthcare model.
The western extension of the Balantak Fault revealed by the 2021 earthquake cascade in the central arm of Sulawesi, Indonesia
Authors: Simanjuntak Andrean V. H. , Palgunadi Kadek H. , Supendi Pepen , Muksin Umar , Gunawan Endra , Widiyantoro Sri , Rawlinson Nicholas , Daryono Mudrik R. , Daryono D. , Karnawati Dwikorita , Hanifa Nuraini R. , Pratama Cecep , Ida Rachmah 
Published: August 2024

AbstractTwo shallow earthquakes of moment magnitude 6.2 and 5.8 occurred in the central arm of Sulawesi on 26 July 2021 and 26 August 2021, respectively. The fault responsible for the earthquake had previously only been partially mapped, thus making further analysis of its characteristics crucial for the assessment of seismic hazard. In this study, we exploit data from a regional seismic network, relocate the associated seismicity using the double difference method with an updated velocity model, determine focal mechanisms from full-waveform inversion, and analyze the static stress changes caused by the mainshock. Our relocated hypocenters and focal mechanism solutions reveal two earthquake clusters, one at the Central Balantak Fault that exhibits normal slip on a SE–NW trending rupture, and the other at the West Balantak Fault, which exhibits dextral strike-slip motion on a SE–NW trending rupture. The additional static stress increase transferred by the Mw 6.2 mainshock may have triggered the subsequent Mw 5.8 event. A detailed assessment of previously unmapped faults in Central Sulawesi is essential for a more comprehensive understanding of seismic hazard in the region.
Seismogenic structures along the deformation front from onshore to offshore SW Taiwan
Authors: Wu Wen-Nan , Lin Jing-Yi , Doo Wen-Bin , Lo Chung-Liang , Hsu Shu-Kun 
Published: August 2024

AbstractAccurately imaging seismogenic structures is crucial for seismic hazard assessment. This is especially important around the deformation front (DF) region off SW Taiwan, where seismic potential is high but the fault structure and seismic activity are still poorly understood. Here, we present a comprehensive delineation of seismogenic structures along the onshore and offshore DF of SW Taiwan, utilizing hypocenter relocations and first-motion focal mechanism solutions from January 1991 to March 2019. Five distinctive seismogenic structures are delineated. Particularly, the energetic seismic activity initialled after the 2017 Mw5.3 Tainan earthquake and migrated northeastward along the western side of the DF. The offshore trace of the DF could serve as a boundary delimiting the seismic activity driven by the flexural stress of the subducted Eurasia Plate to the west of the DF. This observation could constrain the magnitude estimation of future earthquakes offshore SW Taiwan. Meanwhile, the areas with higher seismic moment release are located near the DF rather than known faults, indicating that the DF may dominate the seismic moment release. However, further study is warranted into the intricate relationship among seismic strain variation, the structure of DF, and seismic activity to further understand the seismic potential. Our first-motion focal mechanism solutions show that several earthquakes in SW Taiwan were characterized by P-axis orientations parallel to the strike of the Taiwan orogeny. Those events are preferably explained by the lateral compression induced by the ongoing collision between the Eurasia and Philippine Sea plates.
Dissecting reactive astrocyte responses: lineage tracing and morphology-based clustering
Authors: Delgado-García Lina M. , Ojalvo-Sanz Ana C. , Nakamura Thabatta K. E. , Martín-López Eduardo , Porcionatto Marimelia , Lopez-Mascaraque Laura 
Published: August 2024

AbstractBrain damage triggers diverse cellular and molecular events, with astrocytes playing a crucial role in activating local neuroprotective and reparative signaling within damaged neuronal circuits. Here, we investigated reactive astrocytes using a multidimensional approach to categorize their responses into different subtypes based on morphology. This approach utilized the StarTrack lineage tracer, single-cell imaging reconstruction and multivariate data analysis. Our findings identified three profiles of reactive astrocyte responses, categorized by their effects on cell size- and shape- related morphological parameters: “moderate”, “strong,” and “very strong”. We also examined the heterogeneity of astrocyte reactivity, focusing on spatial and clonal distribution. Our research revealed a notable enrichment of protoplasmic and fibrous astrocytes within the “strong” and “very strong” response subtypes. Overall, our study contributes to a better understanding of astrocyte heterogeneity in response to an injury. By characterizing the diverse reactive responses among astrocyte subpopulations, we provide insights that could guide future research aimed at identifying novel therapeutic targets to mitigate brain damage and promote neural repair.
Graphical Abstract
Selective embolisation of an idiopathic bronchial artery pseudoaneurysm presenting with recurrent laryngeal nerve palsy: a case report
Authors: Copping R. , Ng N. , Osman S. 
Published: August 2024

Abstract
Background
Bronchial artery pseudoaneurysms (BAP) or aneurysms (BAA) are rare, potentially life-threatening and remain poorly understood. They are most commonly idiopathic but may be associated with a number of other disease processes. Bronchial artery embolisation (BAE) is considered the first line treatment while surgical techniques are reserved for patients with a clear contraindication to embolisation or where anatomical factors preclude an endovascular approach.

Case presentation
We present an interesting case of a 56 year-old male presenting with an idiopathic unruptured right BAP causing clinical and radiological signs of left recurrent laryngeal nerve (RLN) palsy. He was otherwise clinically well with no other reported symptoms and no significant past medical history. There were no significant findings on work-up and investigation. He was ultimately treated successfully with selective transarterial coil embolization of the right bronchial artery. This is an atypical presentation of a rare clinical entity and has not previously been published in the literature to our knowledge.

Conclusions
BAPs and BAAs are highly variable in their presentation, ranging from incidental asymptomatic findings to catastrophic haemorrhage, depending on their location and if they are contained or ruptured. Timely diagnosis and referral to facilitate urgent embolisation is essential to prevent potentially serious clinical sequelae. Endovascular treatment in the form of BAE is considered first line.
Proposals for consideration at IMC12 to modify provisions related solely to fungi in Chapter F of the International Code of Nomenclature for algae, fungi, and plants
Authors: May Tom W. , Hawksworth David L. 
Published: August 2024

AbstractSeven proposals or sets of proposals to modify the provisions of Chapter F of the International Code of Nomenclature for algae, fungi, and plants (ICNafp) have been received. These proposals are formally presented together here. The topics addressed relate to: fungi whose morph-names have the same epithet; the listing of synonyms under entries for protected names in the Code Appendices; the processes of protection and rejection; the use of DNA sequences as nomenclatural types; the use of genomes as nomenclatural types; and the designation of fungi known only from DNA sequences. In addition, a suggestion is included to update the mention of the World Directory of Culture Collections in Article 40.7 Note 4. A Synopsis of the formal proposals will be provided in early July 2024, and the mycological community will be invited to provide a guiding vote on the proposals with a closing date of 2 August 2024. Final decisions on the proposals will be made following debate at the Fungal Nomenclature Session of IMC12 in August 2024.
Local community engagement and gazettement approach in managing and conserving Pangani historic town in Tanzania
Authors: Chami Maximilian Felix , Mjema Elinaza 
Published: August 2024

AbstractPangani is a historic coastal Swahili town situated at the mouth of the Pangani River, which empties its waters into the Indian ocean. Archaeologists have traced the origins of this town back to the seventh century through evidence from material cultures found in this area. Despite its historical importance and archaeological interest, Pangani faces substantial conservation and management challenges, primarily due to low awareness levels among its local community. These residents are largely unaware of the town’s historical value and of the impact of factors such as climate change, stone quarrying, wave erosion, and development pressures, so effective conservation plans are lacking. In finding the best practices for managing and conserving this historic town, a critical question remains as to how to rescue the town's significance and value from these challenges. This paper proposes a gazettement approach for managing and conserving Pangani, emphasising a bottom-up approach that involves local communities and other stakeholders in the conservation process.
Separation of microplastics from deep-sea sediment using an affordable, simple to use, and easily accessible density separation device
Authors: Shaw Katherine R. , Sandquist Rachel , Fairclough Cameron , Black Jesse , Fitzgerald Alexandra , Shaw Jaxson T. , Gallager Scott , Lynch Jennifer 
Published: August 2024

AbstractMicroplastics accumulate in the environment but methods to extract particles from sediment for quantification and identification often lack accuracy and reproducibility. Existing methods vary greatly and many do not achieve adequate microplastic separation. During method development for extraction procedures, spike-recovery experiments (positive controls) are essential to ensure accurate and reproducible results from each sample matrix. Furthermore, the large variability in grain size and organic matter can affect the extraction of microplastics from the matrix. Scientists have used density separation to separate microplastics from matrices for decades, but apparatuses are often made of plastic, need to be custom made, and require multiple sample transfers from one apparatus to another. This study presents an affordable, easily accessible, and simple to use Density Separation Device (DSD) to remove plastics from deep-sea sediments. Eight polymers were spiked into replicates of environmental sediment, including six fragments: high density polyethylene (HDPE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), nylon (PA6), and crumb rubber (CR) and two fibers: cellulose acetate (CA) and polyester (PEST). Two size classes of polymers were used: 100 μm to 300 μm and > 300 μm. Using a sodium polytungstate solution at a density of 1.9 g/mL and reflectance FTIR microscopy for particle identification, mean recoveries of all fragments exceeded 78% (CR: 92.7% ± 30.8%, PP: 78.4% ± 34.0%, HDPE: 93.8% ± 13.5%, PS: 86.9% ± 25.7%, PA6: 98.4% ± 63.2%, PVC: 100.0% ± 12.4%). Fiber recovery was much lower (PEST: 28.1% ± 28.1% and CA: 25.9% ± 17.3%) because they aggregated, passed through sieves vertically, or were obscured under other particles. The fragment recovery success, accessibility (available online, all parts under $200) and ease of use of this DSD should facilitate widespread use, thus helping to standardize sample preparation methods for microplastic metrology.
Assessment of Hearing Dysfunction in Patients With Graves’ Disease and Thyroid Eye Disease Without or With Teprotumumab
Authors: Smith Terry J , Holt Robert J , Fu Qianhong , Qashqai Anahita , Barretto Naina , Conrad Elizabeth , Brant Jason A 
Published: August 2024

Abstract

Context
Thyroid eye disease (TED) negatively affects quality of life. TED occurs predominantly in Graves’ disease (GD). Teprotumumab improves TED but concern for hearing adverse events (AEs) has emerged. Hearing dysfunction is reported in thyroid autoimmune disease but the background prevalence in GD/TED without teprotumumab remains uncertain.

Objective
This work aimed to quantify ear-related diagnostic codes/hearing AEs in GD, TED, and patients receiving teprotumumab by examining medical claims and clinical trials.

Methods
Deidentified claims for ear/labyrinth-related International Classification of Disorders, Tenth Revision codes (KOMODO) were examined in GD patients without TED, and TED patients without/with teprotumumab treatment. Hearing AE incidence/severity was evaluated in teprotumumab clinical trials. Graves’ Ophthalmopathy Quality of Life questionnaire (GO-QOL) scores were compared in teprotumumab TED trial patients without/with hearing AEs.

Results
GD (469 720), TED (38 566) and teprotumumab-treated (967) patients were identified in the claims database. Ear-related codes (including those not specific for hearing) occurred in 24% GD, 33% TED, and 32% teprotumumab-treated patients. “Sensorineural hearing loss bilateral” was most frequent: 7% (32 961/469 720) GD, 11.1% (4279/38 566) TED, and 10.8% (104/967) teprotumumab patients. Before teprotumumab use, 17.1% (165) patients had ear-related codes, while 10.1% (98) had new ear-related codes post treatment.
Eight teprotumumab oncology trials revealed 8.1% (51/633) had ear/labyrinth disorders with 2.1% (13) considered study-drug-related and 3.8% (24) hearing impairment/tinnitus-related AEs with 1.3% (8) considered study-drug-related. Similar rates occurred in TED trials.
GO-QOL improved in teprotumumab-treated patients without/with hearing AEs. Incidence/severity was consistent across patients with chronic and acute TED.

Conclusion
These analyses indicate similar prevalence of hearing claims in patients with GD/TED alone as following teprotumumab treatment. Future analyses of incremental hearing risk from teprotumumab should use a priori study designs accounting for background hearing dysfunction in patients with GD/TED.
Compensation of gene dosage on the mammalian X
Authors: Cecalev Daniela , Viçoso Beatriz , Galupa Rafael 
Published: August 2024

ABSTRACT
Changes in gene dosage can have tremendous evolutionary potential (e.g. whole-genome duplications), but without compensatory mechanisms, they can also lead to gene dysregulation and pathologies. Sex chromosomes are a paradigmatic example of naturally occurring gene dosage differences and their compensation. In species with chromosome-based sex determination, individuals within the same population necessarily show ‘natural’ differences in gene dosage for the sex chromosomes. In this Review, we focus on the mammalian X chromosome and discuss recent new insights into the dosage-compensation mechanisms that evolved along with the emergence of sex chromosomes, namely X-inactivation and X-upregulation. We also discuss the evolution of the genetic loci and molecular players involved, as well as the regulatory diversity and potentially different requirements for dosage compensation across mammalian species.
Compromised COPII vesicle trafficking leads to glycogenic hepatopathy
Authors: Yang Yuxi , Zhang Xue , Zhao Qingshun , Zhang Jingzi , Lou Xin 
Published: August 2024

ABSTRACT
Being a vital cellular process, coat protein complex II (COPII) vesicle trafficking has been found to play a crucial role in liver metabolism. However, its functions and the underlying mechanisms in systemic metabolic homeostasis have not been fully understood. Here, with a newly identified gene trap zebrafish line (sec31anju221), we show that compromised COPII vesicle trafficking leads to biphasic abnormal hepatic metabolism. During the larval stage, deficiency of COPII-mediated trafficking leads to activation of the unfolded protein response and the development of hepatic steatosis. By using epistasis analysis, we found that the eIF2α–ATF4 pathway serves as the primary effector for liver steatosis. In adult sec31anju221 fish, the hepatosteatosis was reversed and the phenotype switched to glycogenic hepatopathy. Proteomic profiling and biochemical assays indicate that sec31anju221 fish are in a state of hypothyroidism. Moreover, our study shows that thyroid hormone treatment alleviates the metabolic defects. This study provides insights into processes of liver diseases associated with vesicle trafficking impairments and expands our understanding of the pathological interplay between thyroid and liver.
Computational modeling establishes mechanotransduction as a potent modulator of the mammalian circadian clock
Authors: Francis Emmet A. , Rangamani Padmini 
Published: August 2024

ABSTRACT
Mechanotransduction, which is the integration of mechanical signals from the external environment of a cell to changes in intracellular signaling, governs many cellular functions. Recent studies have shown that the mechanical state of the cell is also coupled to the cellular circadian clock. To investigate possible interactions between circadian rhythms and cellular mechanotransduction, we have developed a computational model that integrates the two pathways. We postulated that translocation of the transcriptional regulators MRTF (herein referring to both MRTF-A and MRTF-B), YAP and TAZ (also known as YAP1 and WWTR1, respectively; collectively denoted YAP/TAZ) into the nucleus leads to altered expression of circadian proteins. Simulations from our model predict that lower levels of cytoskeletal activity are associated with longer circadian oscillation periods and higher oscillation amplitudes, which is consistent with recent experimental observations. Furthermore, accumulation of YAP/TAZ and MRTF in the nucleus causes circadian oscillations to decay in our model. These effects hold both at the single-cell level and within a population-level framework. Finally, we investigated the effects of mutations in YAP or lamin A, the latter of which result in a class of diseases known as laminopathies. In silico, oscillations in circadian proteins are substantially weaker in populations of cells with mutations in YAP or lamin A, suggesting that defects in mechanotransduction can disrupt the circadian clock in certain disease states; however, reducing substrate stiffness in the model restores normal oscillatory behavior, suggesting a possible compensatory mechanism. Thus, our study identifies that mechanotransduction could be a potent modulatory cue for cellular clocks and that this crosstalk can be leveraged to rescue the circadian clock in disease states.
Hominini-specific regulation of the cell cycle by stop codon readthrough of FEM1B
Authors: Akhtar Md Noor , Singh Anumeha , Manjunath Lekha E. , Dey Dhruba , Kumar Sangeetha Devi , Vasu Kirtana , Das Arpan , Eswarappa Sandeep M. 
Published: August 2024

ABSTRACT
FEM1B is a substrate-recognition component of the CRL2 E3 ubiquitin-protein ligase. This multi-protein complex targets specific proteins for ubiquitylation, which leads to their degradation. Here, we demonstrate the regulation of FEM1B expression by stop codon readthrough (SCR). In this process, translating ribosomes readthrough the stop codon of FEM1B to generate a C-terminally extended isoform that is highly unstable. A total of 81 nucleotides in the proximal 3′UTR of FEM1B constitute the necessary and sufficient cis-signal for SCR. Also, they encode the amino acid sequence responsible for the degradation of the SCR product. CRISPR-edited cells lacking this region, and therefore SCR of FEM1B, showed increased FEM1B expression. This in turn resulted in reduced expression of SLBP (a target of FEM1B-mediated degradation) and replication-dependent histones (target of SLBP for mRNA stability), causing cell cycle delay. Evolutionary analysis revealed that this phenomenon is specific to the genus Pan and Homo (Hominini). Overall, we show a relatively recently evolved SCR process that relieves the cell cycle from the negative regulation by FEM1B.
Considering ultraviolet radiation in experimental biology: a neglected pervasive stressor
Authors: Hird Coen , Lundsgaard Niclas U. , Downie Adam T. , Cramp Rebecca L. , Franklin Craig E. 
Published: August 2024

ABSTRACT
Ultraviolet radiation (UVR) is a pervasive factor that has shaped the evolution of life on Earth. Ambient levels of UVR mediate key biological functions but can also cause severe lethal and sublethal effects in a wide range of organisms. Furthermore, UVR is a powerful modulator of the effects of other environmental factors on organismal physiology, such as temperature, disease, toxicology and pH, among others. This is critically important in the context of global change, where understanding the effects of multiple stressors is a key challenge for experimental biologists. Ecological physiologists rarely afford UVR discussion or include UVR in experimental design, even when it is directly relevant to their study system. In this Commentary, we provide a guide for experimental biologists to better understand if, when, and how UVR can be integrated into experimental designs to improve the ecological realism of their experiments.