bluuebunny/crossref_metadata_2025_split huggingface dataset:
The therapeutic potential of indole hybrids, dimers, and trimers against drug‐resistant ESKAPE pathogens
Authors: Qiongxian Ye , Jun Deng , Zhenfeng Zhang , Tongyou Luo , Zhicong Tan , Zhenyou Tan 
Published: June 2024

AbstractEnterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter (ESKAPE) species as causative agents are characterized by increased levels of resistance toward multiple classes of first‐line as well as last‐resort antibiotics and represent serious global health concerns, creating a critical need for the development of novel antibacterials with therapeutic potential against drug‐resistant ESKAPE species. Indole derivatives with structural and mechanistic diversity demonstrated broad‐spectrum antibacterial activity against various clinically important pathogens including drug‐resistant ESKAPE. Moreover, several indole‐based agents that are exemplified by creatmycin have already been used in clinics or under clinical trials for the treatment of bacterial infections, demonstrating that indole derivatives hold great promise for the development of novel antibacterials. This review is an endeavor to highlight the current scenario of indole hybrids, dimers, and trimers with therapeutic potential against drug‐resistant ESKAPE pathogens, covering articles published from 2020 to the present, to open new avenues for the exploration of novel antidrug‐resistant ESKAPE candidates.
Targeted Chemo‐Phototherapy in Red Light with Novel Doxorubicin and Iron(III) Complex‐Functionalized Gold Nanoconjugate (Dox‐Fe@FA‐AuNPs)
Authors: Pal Maynak , Bera Arpan , Masarkar Neha , Upadhyay Aarti , Mukherjee Sukhes , Roy Mithun 
Published: June 2024

AbstractThe anticancer efficacy of doxorubicin, an anthracycline‐based and FDA‐approved chemotherapeutic drug, is significantly hindered by acquired chemoresistance and severe side effects despite its potent anticancer properties. To overcome these challenges, we developed an innovative therapeutic formulation that integrates targeted chemotherapy and phototherapy within a single platform using gold nanoparticles (AuNPs). This novel nanoconjugate, designated as Dox‐Fe@FA‐AuNPs, is co‐functionalized with folic acid, doxorubicin, and an iron(III)‐phenolate/carboxylate complex, enabling cancer‐specific drug activation. Here, we report the synthesis, characterization, and comprehensive physico‐chemical and biological evaluations of Dox‐Fe@FA‐AuNPs. The nanoconjugate exhibited excellent solubility, stability, and enhanced cellular uptake in folate receptor‐positive cancer cells. The nanoconjugate was potently cytotoxic against HeLa and MDA‐MB‐231 cancer cells (HeLa: 105.5±16.52 μg mL−1; MDA‐MB‐231: 112.0±12.31 μg mL−1; MDA‐MB‐231 (3D): 156.31±19.35 μg mL−1) while less cytotoxic to the folate(−) cancer cells (MCF‐7, A549 and HepG2). The cytotoxicity was attributed to the pH‐dependent release of doxorubicin, which preferentially occurs in the acidic tumor microenvironment. Additionally, under red light irradiation, the nanoconjugate generated ROS, inducing caspase‐3/7‐dependent apoptosis with a photo‐index (PI) >50, and inhibited cancer cell migration. Our findings underscore the potential of Dox‐Fe@FA‐AuNPs as a highly effective and sustainable platform for targeted chemo‐phototherapy.
Understanding the potential of bamboo fibers in the USA: A comprehensive techno‐economic comparison of bamboo fiber production through mechanical and chemical processes
Authors: Vivas Keren A. , Pifano Alonzo , Vera Ramon E. , Urdaneta Fernando , Urdaneta Isabel , Forfora Naycari , Abbati de Assis Camilla , Phillips Richard B. , Dasmohapatra Sudipta , Saloni Daniel , Venditti Richard A. , Gonzalez Ronalds 
Published: June 2024

AbstractThe growing interest in bamboo fibers for pulp, paper, and board production in the USA necessitates a comprehensive financial viability assessment. This study conducts a detailed technoeconomic analysis (TEA) of bamboo fiber production, primarily for the consumer hygiene tissue market although it is also applicable to other industrial uses. The economic viability of two pulping methods – alkaline peroxide mechanical pulping (APMP) and ammonium bisulfite chemical pulping (ABS) – was explored within three different pulp mill settings to supply pulp to two nonintegrated tissue and towel mills in South Carolina, USA. The target was to produce wet lap bamboo bleached pulp at 50% consistency and 70% ISO brightness. Despite higher initial capital invesment and operating costs, ABS achieved a lower minimum required selling price – USD 544 to 686 per bone dry metric ton (BDt = 1000 BDkg) – in comparison with USD 766 to 899 BDt−1 for APMP. This price advantage is partly due to an additional revenue stream (lignosulfonate byproduct), which not only boosts revenue but also circumvents the need for expensive chemical recovery systems. When compared with traditional kraft pulping, both methods require significantly lower capital investments, with minimum required selling prices (estimated to achieve 16% IRR) below current market rates for extensively used bleached kraft pulps in the USA tissue industry. The economic benefits derive from several factors: the low cost of bamboo as raw material, reduced capital needs for new pulping technologies, lower transportation costs from the pulp mill to tissue and towel manufacturing facilities, and the high market price of bleached kraft pulp.
The concept of balance in microbiome research
Authors: O'Malley Maureen A. 
Published: June 2024

AbstractMicrobiome research is changing how ecosystems, including animal bodies, are understood. In the case of humans, microbiome knowledge is transforming medical approaches and applications. However, the field is still young, and many conceptual and explanatory issues need resolving. These include how microbiome causality is understood, and how to conceptualize the role microbiomes have in the health status of their hosts and other ecosystems. A key concept that crops up in the medical microbiome literature is “balance.” A balanced microbiome is thought to produce health and an imbalanced one disease. Based on a quantitative and qualitative analysis of how balance is used in the microbiome literature, this “think again” essay critically analyses each of the several subconceptions of balance. As well as identifying problems with these uses, the essay suggests some starting points for filling this conceptual gap in microbiome research.
Preparation of nanocellulose/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles for efficient catalytic reduction of 4‐nitrophenol
Authors: Khili Faouzia , Omrani Amel Dakhlaoui 
Published: June 2024

AbstractThe paper reports on the preparation of cellulose nanocrystals/reduced graphene oxide matrix loaded with cuprous oxide nanoparticles (CNC/rGO‐Cu2O) through a simple solvothermal method and its application for 4‐nitrophenol reduction to 4‐aminophenol using sodium borohydride. The CNC/rGO‐Cu2O nanocomposite was formed chemically by first mixing CNC and graphene oxide (GO) followed by complexation of the negatively charged functional groups of CNC/GO with Cu2+ ions and subsequent heating at 100°C. This resulted in the simultaneous reduction of GO to rGO and the formation of Cu2O nanoparticles. The as‐elaborated nanocomposite was firstly characterized using different techniques such as atomic force microscopy, scanning electron microscopy, transmission electron microscopy, UV–Vis spectrophotometry, Raman spectroscopy and x‐ray photoelectron spectroscopy. Then, it was successfully applied for efficient catalytic reduction of 4‐nitrophenol to 4‐aminophenol using sodium borohydride: the reduction was completed in about 6 min. After eight times use, the catalyst still maintained good catalytic performance. Compared to CNC/rGO, rGO/Cu2O and free Cu2O nanoparticles, the CNC/rGO‐Cu2O nanocomposite exhibits higher catalytic activity even at lower copper loading.
α‐Glucosidase Inhibitory Activity of Prenylated Pyranocoumarins from Clausena excavata: Mechanism of Action, ADMET and Molecular Docking
Authors: Promden Worrawat , Lophaet Aphiwat , Sripadung Ployvadee , Sungthong Bunleu , Samsee Thanatcha , Ploylearmsang Chanuttha , Kijjoa Anake , Seephonkai Prapairat 
Published: June 2024

AbstractThree naturally occurring prenylated pyranocoumarins, nordentatin (1), dentatin (2), and clausarin (3), isolated from the roots of Clausena excavata (Family Rutaceae), and O‐methylclausarin (4) which was obtained by methylation of 3, were investigated for their α‐glucosidase inhibitory activity. The mechanism of action and the in silico prediction of their physicochemical and ADMET properties as well as the molecular docking were also studied. Compounds 1–4 exhibited stronger α‐glucosidase inhibitory activity than the positive control, acarbose, through a non‐competitive mechanism. Among them, 3 exhibited the highest activity, with an IC50 of 8.36 μM, which is significantly stronger than that of acarbose (IC50=430.35 μM). The prenyl group on C‐3 and the hydroxyl group on C‐5 in 3 may play important roles in enhancing the activity. Calculated physicochemical and ADMET parameters of 1–4 satisfied the Lipinski's and Veber's rules. Molecular simulation analysis indicated they are promising drug candidates with no hepatotoxicity. Compound 3 exhibited potent activity in the experiment and demonstrated good drug properties based on the calculations. A molecular docking study revealed that 3 showed H‐bonding and π‐π stacking interactions with selective Phe321, as well as interactions with thirteen other amino acid residues of the α‐glucosidase.
Impact of intravascular ultrasound‐guided endovascular therapy on periprocedural outcomes in patients with lower‐extremity artery disease
Authors: Nakao Sho , Takahara Mitsuyoshi , Iida Osamu , Kohsaka Shun , Amano Tetsuya , Ishii Hideki , Shinke Toshiro , Nakama Tatsuya , Fujihara Masahiko , Mano Toshiaki , Kozuma Ken 
Published: June 2024

AbstractBackgroundAlthough technological improvements in intravascular ultrasound (IVUS) may reduce technical failures in endovascular therapy (EVT), perioperative complications (POCs) associated with IVUS use may increase.AimsThis study investigated the impact of IVUS on periprocedural outcomes in symptomatic lower‐extremity artery disease (LEAD) patients undergoing EVT.MethodsThis study evaluated 28,088 symptomatic LEAD patients who underwent EVT between January 2021 and December 2021 using a prospective nationwide registry in Japan. Outcome measures included periprocedural outcomes, including technical failure and POCs. To compare outcomes with and without IVUS use, propensity score matching analysis was performed for overall and for each arterial region (aortoiliac [AI], femoropopliteal [FP], and infrapopliteal [IP] arteries) using a binary logistic regression model.ResultsIVUS was used in 75%, 72%, and 37% of AI, FP, and IP lesions, respectively. After propensity matching extraction, the IVUS group had a tendency of lower technical failure rate than the non‐IVUS group, although not statistically different (3.9% vs. 5.4%, p = 0.054), without an increase in the POC rate (1.8% vs. 1.6%, p = 0.54). Regarding the per‐regional analysis, the technical failure rate of FP‐EVT was significantly lower in the IVUS group (3.1% vs. 4.2%, p = 0.006), whereas those of AI‐EVT (2.2% vs. 3.1%, p = 0.12) and IP‐EVT (6.8% vs. 6.1%, p = 0.37) were not significantly different between the two groups. Furthermore, IVUS did not increase the POC rate for any region (AI‐EVT: 1.3% vs. 1.3%, p = 1.00; FP‐EVT: 1.8% vs. 1.7%, p = 0.75; and IP‐EVT: 2.0% vs. 1.7%, p = 0.56).ConclusionThe current study revealed that IVUS did not increase the POCs and technical failure for overall lesions but reduced the incidence of FP‐EVT technical failure.
Intentional replantation for a Class 4/Stage C cemental tear: Etiology, choice and outcomes of treatment
Authors: Luo Ning , Ying Xuan , Tong Yuxin , Chen Yue 
Published: June 2024

Key Clinical MessageCemental tears, root cracks, and associated periapical‐periodontal lesions may occur simultaneously in one tooth, and can be effectively managed by intentional replantation and etiological control. A durable splint, along with occlusal and periodontal monitoring, is required as healing progresses slowly and may be insufficient.
26 cm fall caught on video causing subdural hemorrhages and extensive retinal hemorrhages in an 8‐month‐old infant
Authors: Brook Chris , Squier Waney , Mack Julie 
Published: June 2024

Key Clinical MessageSevere, too many to count retinal hemorrhages (RH) in infants have been associated with abusive head trauma, but can occur in short falls. An 8‐month‐old male fell backward from a height of 26 cm, landing on his buttocks then hitting the back of his head on a vinyl floor. The fall was videotaped. Acute subdural hemorrhages were found along with extensive, too many to count intra‐RH in both eyes. Falls from small heights on to the occiput can lead to extensive RH of the type often associated with abusive head trauma.
Chiral Brønsted Acid‐Catalyzed Asymmetric Reaction via Vinylidene Ortho‐Quinone Methides
Authors: Zhu Xin‐Qi , Yang Hai‐Yu , Ye Long‐Wu 
Published: June 2024

AbstractVinylidene ortho‐quinone methides (VQMs) have been proven to be versatile and crucial intermediates in the catalytic asymmetric reaction in last decade, and thus have drawn considerable concentrations on account of the practical application in the construction of enantiomerically pure functional organic molecules. However, in comparison to the well established chiral Brønsted base‐catalyzed asymmetric reaction via VQMs, chiral Brønsted acid‐catalyzed reaction is rarely studied and there is no systematic summary to date. In this review, we summarize the recent advances in the chiral Brønsted acid‐catalyzed asymmetric reaction via VQMs according to three types of reactions: a) intermolecular asymmetric nucleophilic addition to VQMs; b) intermolecular asymmetric cycloaddition of VQMs; c) intramolecular asymmetric cyclization of VQMs. Finally, we put forward the remained challenges and opportunities for potential breakthroughs in this area.
Chemical Reaction Steers Spatiotemporal Self‐Assembly of Supramolecular Hydrogels
Authors: Wang Hucheng , Bai Shengyu , Gu Guanyao , Zhang Chunyu , Wang Yiming 
Published: June 2024

AbstractSupramolecular structures are widespread in living system, which are usually spatiotemporally regulated by sophisticated metabolic processes to enable vital biological functions. Inspired by living system, tremendous efforts have been made to realize spatiotemporal control over the self‐assembly of supramolecular materials in synthetic scenario by coupling chemical reaction with molecular self‐assembly process. In this review, we focused on the works related to supramolecular hydrogels that are regulated in space and time using chemical reaction. Firstly, we summarized how spatially controlled self‐assembly of supramolecular hydrogels can be achieved via chemical reaction‐instructed self‐assembly, and the application of such a self‐assembly methodology in biotherapy was discussed as well. Second, we reviewed dynamic supramolecular hydrogels dictated by chemical reaction networks that can evolve their structures and properties against time. Third, we discussed the recent progresses in the control of the self‐assembly of supramolecular hydrogels in both space and time though a reaction‐diffusion‐coupled self‐assembly approach. Finally, we provided a perspective on the further development of spatiotemporally controlled supramolecular hydrogels using chemical reaction in the future.
Total Synthesis of an Epothilone Analogue Based on the Amide‐Triazole Bioisosterism
Authors: Colombo Eleonora , Coppini Davide A. , Borsoi Simone , Fasano Valerio , Bucci Raffaella , Bonato Francesca , Bonandi Elisa , Vasile Francesca , Pieraccini Stefano , Passarella Daniele 
Published: June 2024

AbstractEpothilones are 16‐membered macrolides that act as microtubule‐targeting agents to tackle cancer. Many synthetic analogues have been investigated for their activity, yet often based on macrolide structures. A notable exception is Ixabepilone, an azalide whose metabolic stability and pharmacokinetics are significantly improved. Exploiting the amide‐triazole bioisosterism, in this work we report the synthesis of the first generation of epothilones lacking the macrolide or azalide structure, with the ester or amide linkage replaced by a triazole unit. Together with the synthesis of this new analogue, computational and biological evaluations have been performed too.
The Affective Bias Test and Reward Learning Assay: Neuropsychological Models for Depression Research and Investigating Antidepressant Treatments in Rodents
Authors: Hinchcliffe Justyna K. , Robinson Emma S. J. 
Published: June 2024

AbstractThe Affective Bias Test (ABT) quantifies acute changes in affective state based on the affective biases they generate in an associative reward learning task. The Reward Learning Assay (RLA) provides a control assay for the ABT and reward‐induced biases generated in this model are sensitive to changes in core affective state. Both tasks involve training animals to associate a specific digging substrate with a food reward. Animals learn to discriminate between two digging substrates placed in ceramic bowls, one rewarded and one unrewarded. In the ABT, the animal learns two independent substrate‐reward associations with a fixed reward value following either an affective state or drug manipulation, or under control conditions. Affective biases generated are quantified in a choice test where the animals exhibit a bias (make more choices) for one of the substrates which is specifically related to affective state at the time of learning. The ABT is used to investigate biases generated during learning as well as modulation of biases associated with past experiences. The RLA follows a similar protocol, but the animal remains in the same affective state throughout and a reward‐induced bias is generated by pairing one substrate with a higher value reward. The RLA provides a control to determine if drug treatments affect memory retrieval more generally. Studies in depression models and following environmental enrichment suggest that reward‐induced biases are sensitive to core changes in affective state. Each task offers different insights into affective processing mechanisms and may help improve the translational validity of animal studies and benefit pre‐clinical drug development. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Bowl digging and discrimination trainingBasic Protocol 2: The reward learning assayBasic Protocol 3: The affective bias test ‐ new learningBasic Protocol 4: The affective bias test ‐ modulation of affective biases associated with past experiences
Morphologically defined substages of tail morphogenesis in C. elegans males
Authors: Kiontke Karin , Fernandez Porfirio , Woronik Alyssa , Fitch David H. A. 
Published: June 2024

AbstractBackgroundSex‐specific morphogenesis occurs in Caenorhabditis elegans in the vulva of the hermaphrodite and in the male tail during the last larval stage. Temporal progression of vulva morphogenesis has been described in fine detail. However, a similar precise description of male tail morphogenesis was lacking.ResultsWe here describe morphogenesis of the male tail at time points matching vulva development with special focus on morphogenesis of the tail tip. Using fluorescent reporters, we follow changes in cell shapes, cell fusions, nuclear migration, modifications in the basement membrane, and formation of a new apical extracellular matrix at the end of the tail.ConclusionOur analysis answers two open questions about tail tip morphogenesis (TTM) by showing that one of the four tail tip cells, hyp11, remains largely separate, while the other cells fully fuse with each other and with two additional tail cells to form a ventral tail syncytium. This merger of cells begins at the apical surface early during TTM but is only completed toward the end of the process. This work provides a framework for future investigations of cell biological factors that drive male tail morphogenesis.
Microbial vectoring capacity by internal‐ and external‐infesting stored product insects after varying dispersal periods between novel food patches: An underestimated risk
Authors: Ponce Marco A. , Maille Jacqueline M. , Stoll Ian , James Avery , Bruce Alexander , Kim Tania N. , Scully Erin D. , Morrison William R. 
Published: June 2024

AbstractUnderstanding the ability of internal‐ and external‐infesting stored product insects to vector microbes is important for estimating the relative risk that insects pose to postharvest commodities as they move between habitat patches and in the landscape. Thus, the aim of the current study was to evaluate and compare the microbial growth in novel food patches at different dispersal periods by different populations of Sitophilus oryzae (e.g., internal‐infesting) and Lasioderma serricorne (e.g., external‐infesting). Adults of both species collected from laboratory colonies or field‐captured populations were either placed immediately in a novel food patch, or given a dispersal period of 24 or 72 h in a sterilized environment before entering a surrogate food patch. Vectored microbes in new food patches were imaged after 3 or 5 days of foraging, and microbial growth was processed using ImageJ while fungal species were identified through sequencing the ITS4/5 ribosomal subunit. We found that increasing dispersal time resulted in multiple‐fold reductions in microbial growth surrogate food patches by L. serricorne but not S. oryzae. This was likely attributable to higher mobility by S. oryzae than L. serricorne. A total of 20 morphospecies were identified from 13 genera among the 59 sequences, with a total of 23% and 16% classified as Aspergillus and Penicillium spp. Our data suggest that there is a persistent risk of microbial contamination by both species, which has important food safety implications at food facilities.
Forecasting climate change impacts on neotropical Myotis: Insights from ecological niche models for conservation strategies
Authors: de Oliveira Karoliny , Novaes Roberto Leonan M. , Weber Marcelo M. , Moratelli Ricardo 
Published: June 2024

AbstractMyotis originated during the Oligocene in Eurasia and has become one of the most diverse bat genera, with over 140 species. In the case of neotropical Myotis, there is a high degree of phenotypic conservatism. This means that the taxonomic and geographic limits of several species are not well understood, which constrains detailed studies on their ecology and evolution and how to effectively protect these species. Similar to other organisms, bats may respond to climate change by moving to different areas, adapting to new conditions, or going extinct. Ecological niche models have become established as an efficient and widely used method for interpolating (and sometimes extrapolating) species' distributions and offer an effective tool for identifying species conservation requirements and forecasting how global environmental changes may affect species distribution. How species respond to climate change is a key point for understanding their vulnerability and designing effective conservation strategies in the future. Thus, here, we assessed the impacts of climate change on the past and future distributions of two phylogenetically related species, Myotis ruber and Myotis keaysi. The results showed that the species are influenced by changes in temperature, and for M. ruber, precipitation also becomes important. Furthermore, M. ruber appears to have been more flexible to decreases in temperature that occurred in the past, which allowed it to expand its areas of environmental suitability, unlike M. keaysi, which decreased and concentrated these areas. However, despite a drastic decrease in the spatial area of environmental suitability of these species in the future, there are areas of potential climate stability that have been maintained since the Pleistocene, indicating where conservation efforts need to be concentrated in the future.