bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lifetime congenital isolated GH deficiency does not protect from the development of diabetes
Authors: Vicente Taísa A R , Rocha Ívina E S , Salvatori Roberto , Oliveira Carla R P , Pereira Rossana M C , Souza Anita H O , Campos Viviane C , Santos Elenilde G , Diniz Rachel D C Araújo , Valença Eugênia H O , Epitácio-Pereira Carlos C , Oliveira Mario C P , Mari Andrea , Aguiar-Oliveira Manuel H 
Published: May 2013

ObjectivesAdult subjects with untreated, lifetime, isolated GH deficiency (IGHD) due to a homozygous GHRH receptor gene mutation (MUT/MUT) residing in Itabaianinha, Brazil, present with lower BMI, higher prevalence of impaired glucose tolerance (IGT), increased insulin sensitivity (IS), and reduced β-cell function (βCF) when compared with non-BMI-matched homozygous normal controls. However, the prevalence of diabetes mellitus (DM) in this cohort is unknown. Comparing their IS and βCF with BMI-matched individuals heterozygous for the same mutation (MUT/N) may be useful to elucidate the role of the GH–IGF1 axis in IS and βCF. The purposes of this work were to verify the prevalence of IGT and DM in adult MUT/MUT subjects from this kindred and to compare IS and βCF in MUT/MUT and MUT/N.DesignCross-sectional study.MethodsWe studied most (51) of the living IGHD adults of this kindred who are GH naive. The oral glucose tolerance test (OGTT) could be performed in 34 subjects, fasting glucose was measured in 15, while two had a previous diagnosis of DM. The OGTT results of 24 MUT/MUT subjects were compared with those of 25 BMI-matched MUT/N subjects. IS was assessed by homeostatic model assessment of insulin resistance (HOMA–IR), quantitative IS check index, and oral glucose IS index for 2 and 3 h. βCF was assayed by HOMA-β, insulinogenic index, and the area under the curve of insulin:glucose ratio.ResultsThe prevalence of DM and IGT in IGHD was 15.68 and 38.23% respectively. IS was increased and βCF was reduced in MUT/MUT in comparison with MUT/N.ConclusionsLifetime, untreated IGHD increases IS, impairs βCF, and does not provide protection from diabetes.
Controlled Design of Mesostructured Titania Based Materials
Authors: Soler-Illia G.J. de A.A. , Grosso D. , Crepaldi E. L. , Cagnol F. , Sanchez C. 
Published: May 2013

AbstractMesostructured TiO2-surfactant hybrid xerogels and thin films are prepared by Evaporation-Induced Self Assembly (EISA). These organized structures are reproducibly formed through cooperative self-assembly between hydrophilic Ti-oxo species and a micellar template after selective solvent evaporation from ethanol/HCl/H2O media. The construction of these networks is tailored by tuning the hydrolysis-condensation of the metallic cations with the self-assembly of the organic counterparts (“hydrophilic matching” approach). The hybrids present hexagonal mesostructure (p6m). The formation kinetics of the mesophase is followed by SAXS, interferometry and mass spectrometry (MS) analysis of the vapor phase. An adequate thermal treatment of the organized hybrids leads to high surface (150-400m2 g-1) phosphorus-free mesoporous titania films or powders, with walls containing anatase nanocrystallites. This approach can be successfully extended to other non-silicate based meso-structured materials.
Extraction Socket Healing in Humans After Ridge Preservation Techniques: Comparison Between Flapless and Flapped Procedures in a Randomized Clinical Trial
Authors: Barone Antonio , Toti Paolo , Piattelli Adriano , Iezzi Giovanna , Derchi Giacomo , Covani Ugo 
Published: May 2013

Background: The preservation of hard and soft tissue volume, partially lost after tooth removal, can potentially reduce the need for the more demanding augmentation procedures used in implant‐supported rehabilitation. The objective of this research study is to investigate the effect of filling with xenogeneic material the postextractive sockets of two surgical procedures (flapless versus flapped).Methods: In this prospective randomized clinical survey, two types of socket preservation were performed on two groups of patients: the control, treated via full‐thickness mucoperiosteal flap, and the test, via a flapless procedure. Anatomic measurements and related outcome variables at the third month were analyzed using multiway analysis of variance. Multiple comparison tests, using Tukey honestly significant difference test, and appropriate pairwise comparison tests for independent samples were carried out.Results: Sixty‐four patients were treated, 32 for each of the two socket preservation procedures. Statistically significant differences were registered for the output variables — changes in width of keratinized gingiva, changes in bucco‐lingual width, and vertical bone changes at four sites — between the two socket preservation techniques, with P values of <0.001, <0.001, and 0.0105, respectively.Conclusions: A full‐thickness mucoperiosteal flap gave significantly more negative results than that of the less‐demanding flapless procedure, with an increased width resorption of the postextraction site. Moreover, the increased value of the keratinized gingival width attested to the positive outcome of a flapless procedure in terms of soft tissue preservation and improvement. On the other hand, the flapped technique seemed to show less vertical bone resorption on the buccal aspect than the flapless technique.
Tumor Necrosis Factor‐α Induces Matrix Metalloproteinases‐3, ‐10, and ‐13 in Human Periodontal Ligament Cells
Authors: Ahn Su‐Jin , Rhim Eun‐Mi , Kim Ji‐Yoen , Kim Kyung‐Hee , Lee Hyeon‐Woo , Kim Eun‐Cheol , Park Sang Hyuk 
Published: May 2013

Background: Various biologic mediators, including matrix metalloproteinases (MMPs), that are implicated in periodontal tissue breakdown can be induced by cytokines. MMPs are known to degrade periodontal ligament attachment, and bone matrix proteins and tissue inhibitors of metalloproteinase (TIMPs) inhibit the activity of MMPs. The aim of this study is to investigate the effect of tumor necrosis factor (TNF)‐α on the expression of MMPs in human periodontal ligament (PDL) cells in vitro and establish which MMPs are expressed specifically in response to that stimulus.Methods: Cultured PDL cells were stimulated with TNF‐α and analyzed with an MMP antibody array. Real‐time polymerase chain reaction (PCR), enzyme‐linked immunosorbent assay (ELISA), and western blot with cell lysate and zymography were used to measure messenger RNA (mRNA) and protein levels of MMP‐3, ‐10, and ‐13. To examine TNF receptor (TNFR) expression, PDL cells were examined by flow cytometry, and expression of MMP‐3, ‐10, and ‐13 was observed after blocking the TNFR with an antagonist. Results from real‐time PCR, ELISA, and western blot were analyzed by paired t test.Results: The antibody array showed that the protein most strongly upregulated by TNF‐α stimulation was MMP‐3, followed by MMP‐13 and MMP‐10. The TNF‐α receptor blocker specifically inhibited expression of MMP‐3 and ‐13. In addition, TNF‐α increased levels of MMP mRNAs in MMP‐3, ‐13, and ‐10 (in decreasing order). However, ELISAs showed that MMP‐13 was the most upregulated protein, followed by MMP‐10 and MMP‐3. Western blotting indicated that TNF‐α increased MMP‐3 and ‐13 levels but had no significant effect on the level of MMP‐10, and zymography showed that TNF‐α increased the activities of all forms of MMP‐3 and ‐13, but MMP‐10 was not detected. Flow cytometry demonstrated that the majority of PDL cells expressed TNFR1.Conclusions: TNF‐α (10 ng/mL) upregulates levels of MMP‐3, ‐10, and ‐13 in human PDL cells. These results suggest that these proteins play an important role in the inflammation of PDLs.
Author Characteristics for Major Accounting Journals: Differences among Similarities 1989–2009
Authors: Fogarty Timothy J. , Jonas Gregory A. 
Published: May 2013

ABSTRACT
Many academic accountants have explicit or implicit motivations to publish their research in the best journals in the discipline. However, whether the chances of success are better at some of these journals is unknown. This paper examines the archival record to find differences within the authorship of three such publications (The Accounting Review, Journal of Accounting Research, Journal of Accounting and Economics) over a recently completed 20-year period. The journals do not differentiate according to the authors' doctoral training, but are differently sensitive to place of faculty employment. The journals are equally receptive to non-U.S. authors, but different in their receptivity to recently graduated and frequently appearing authors. Although areas of change over time are noted, both among journals and within each journal itself, the record also shows a good deal of consistency in other relationships over the 20-year period.
Laser scribing on HOPG for graphene stamp printing on silicon wafer
Authors: Butikova Jelena , Polyakov Boris , Dimitrocenko Lauris , Butanovs Edgars , Tale Ivars 
Published: May 2013

Abstract
Highly oriented pyrolytic graphite (HOPG) was scribed by pulsed laser beam to produce square patterns. Patterning of HOPG surface facilitates the detachment of graphene layers during contact printing. Direct HOPG-to-substrate and glue-assisted stamp printing of a few-layers graphene was compared. Printed graphene sheets were visualized by optical and scanning electron microscopy. The number of graphene layers was measured by atomic force microscopy. Glue-assisted stamp printing allows printing relatively large graphene sheets (40×40 μm) onto a silicon wafer. The presented method is easier to implement and is more flexible than the majority of existing ways of placing graphene sheets onto a substrate.
An unexpected position of the dialyses catheter during routine x-ray examination: a case of the double superior vena cava
Authors: Atalić Bruno , Sabo Goran , Toth Jurica 
Published: May 2013

Abstract
This paper presents a case of a 55 year old male patient, who after hospitalization at the Intensive Care Unit, due to acute renal failure, at first had a central venous catheter inserted through his right subclavian vein, and then a dialysis catheter inserted through his left subclavian vein. A routine X-ray examination confirmed that the central venous catheter was visualised in the expected position of the right atrium, which was reached via superior vena cava. The dialysis catheter was not visualised in the expected position of the right atrium, but in the surprising location of the left ventricle, with its line continuously passing by the left sternal edge instead of crossing the middle line in order to enter the superior vena cava, thus raising a concern over its misplacement and possible side-effects (abstract image photo). Due to the absence of pneumo- or haemato-thorax, as the most common signs of the venous rupture, the possible explanation was an anatomical variation. Dialysis catheter displacement in the left internal thoracic vein was proposed as another possibility. Literature research explained it as a case of double superior vena cava, which was confirmed by analysis of the computerised tomography pulmonary angiogram.
An all-fibre multi-parameter sensor for composite structures based on a chirped fibre Bragg grating
Authors: Zhan Y. , Li L. , Yang F. , Gu K. , Wu H. , Yu M. 
Published: May 2013

AbstractAn all-fibre multi-parameter sensor for composite structures based on a chirped optical fibre Bragg grating (CFBG) has been studied theoretically and experimentally. The principle of multi-parameters sensing with a single CFBG is based on that the centre wavelength and the FWHM (full width at half maximum) in the reflection spectrum of a CFBG vary linearly with the temperature and/or the axial stress. A wavelength matched optical fibre long period grating (LPG) and another wavelength matched reference CFBG (CFBGR) have been used to interrogate the signal of the sensor CFBG (CFBGS). The temperature and strain of the composite structure have been monitored real-time with one CFBG as a single sensor head. The temperature resolution of 0.12°C and the strain resolution of 1.1μɛ for a composite structure have been realized in experiments. The experiment results agree well with the theoretical analyses.
Challenges in Planning Long-term Care Menus: That Meet Dietary Recommendations
Authors: Viveky Navita , Billinsky Jennifer , Thorpe Lilian , Alcorn Jane , Hadjistavropoulos Thomas , Whiting Susan J. 
Published: May 2013

Purpose: Long-term care (LTC) homes plan menus based on Eating Well with Canada’s Food Guide (CFG) recommendations for older adults. To determine whether recommended CFG servings and nutrients were being provided, we analyzed the menu of a large LTC facility in a metropolitan area and compared our analysis with a similar one conducted in 2000. Methods: A full week’s menu from a large Saskatoon LTC facility was analyzed and compared with CFG and recent Dietary Recommended Intake nutrient recommendations. The menu was analyzed using The Food Processor SQL. The 2011 menu was compared with the similar 2000 menu analysis to permit an evaluation of changes over a decade. Results: The 2011 menu demonstrated a significant improvement in servings of vegetables and fruit (4.6 to 7.2 servings). Servings of grain products had declined from 4.9 to 3.6 and servings of milk and alternatives had declined from 2.4 to 1.2 since 2000. Servings of meat and alternatives, total carbohydrate, and protein were not significantly different. Foods on the 2011 menu were lower in fat and higher in dietary fibre and offered more vitamins and minerals. Conclusions: Greater attention to the planning of LTC menus may explain improvements in the 2011 LTC menu. The current menu, however, needs to overcome the challenges that prevent it from meeting CFG recommendations for older adults.
Dietetic Students’ Understanding: Of What Becoming a Dietitian Means
Authors: MacLellan Debbie , Lordly Daphne 
Published: May 2013

Purpose: We explored dietetic students’ beliefs about what becoming a dietitian means. Methods: A phenomenological approach to inquiry was used. Thirteen undergraduate students enrolled in a dietetics program were interviewed, and data were analyzed thematically. Results: The overarching theme was students’ expectations of what being a dietitian means and how those expectations change throughout their education. Exposure to a variety of dietitians in practice helped shape participants’ understanding of being a dietitian. Conclusions: For dietetic students to develop a strong identity as dietitians and to strengthen the profession, they must be exposed to practising professionals early and often.
Book Reviews
Authors: Naranjo Gloria Elena , Gal Fabian Andras 
Published: May 2013

Zapata-Barrero, R. y Gallardo-Ferrer, X. (Eds.) (2012). Fronteras en movimiento. Migraciones hacia la Unión Europea en el contexto Mediterráneo.
Reseña por Gloria Elena NaranjoKühnhardt, L. (2010). Region-building: The global proliferation of regional integration (Vol.1).
Review by Fabian Andras Gal
Host B7-H4 Regulates Antitumor T Cell Responses through Inhibition of Myeloid-Derived Suppressor Cells in a 4T1 Tumor Transplantation Model
Authors: Leung Joanne , Suh Woong-Kyung 
Published: May 2013

Abstract
B7-H4, a member of the B7 family of T cell immunomodulatory proteins, has been shown to inhibit T cell responses and neutrophil expansion during bacterial infections. However, the role of B7-H4 in the immune response during tumor growth has been unclear. In this study, we examined the host immune responses in B7-H4–deficient (knockout [KO]) or sufficient (wild-type [WT]) BALB/cJ mice upon transplantation of murine 4T1 carcinoma cells that had little B7-H4 expression. We reveal that host B7-H4 not only dampens the antitumor Th1 responses, but also inhibits the protumor function of myeloid-derived suppressor cells (MDSC). We observed increased expression of both antitumor immune effectors and protumor MDSC-associated transcripts in 4T1 tumors grown in B7-H4 KO mice compared with those grown in WT hosts. Consistently, MDSCs derived from B7-H4 KO mice suppressed T cell proliferation more potently than their WT counterparts. Although the primary growth of 4T1 tumors in B7-H4 KO hosts was similar to that in WT mice, tumors that had grown in B7-H4 KO hosts grew much slower than those from WT mice when subsequently transplanted into WT hosts. Importantly, this differential tumor growth during the secondary transplantation was abrogated when recipient mice lacked T cells, indicating that the immune environment in B7-H4 KO hosts allowed outgrowth of 4T1 tumors with reduced immune-evasive capacities against T cells. Thus, B7-H4 can inhibit both antitumor T cells and protumor MDSCs, influencing the immune-evasive character of the outgrowing tumors. These factors should be considered if B7-H4 blockade is to be used for cancer immunotherapy.
Cross Talk between Follicular Th Cells and Tumor Cells in Human Follicular Lymphoma Promotes Immune Evasion in the Tumor Microenvironment
Authors: Rawal Seema , Chu Fuliang , Zhang Min , Park Hyun Jun , Nattamai Durga , Kannan Shibichakravarthy , Sharma Rakesh , Delgado David , Chou Tina , Lin Heather Y. , Baladandayuthapani Veerabhadran , Luong Amber , Vega Francisco , Fowler Nathan , Dong Chen , Davis R. Eric , Neelapu Sattva S. 
Published: May 2013

Abstract
The microenvironment of human follicular lymphoma (FL), an incurable B cell non-Hodgkin’s lymphoma, is thought to play a major role in its pathogenesis and course. Microenvironmental cells of likely importance include follicular Th cells (TFH) and regulatory T cells (Tregs), and understanding their interactions with FL tumor cells is necessary to develop novel therapeutic strategies. We found that IL-4 and CD40L are expressed by intratumoral TFH and induce production of CCL17 and CCL22 by FL tumor cells. IL-4 alone induces only CCL17 but enhances stimulation by CD40L of both CCL17 and CCL22. Consistent with our in vitro results, mRNA transcripts of IL-4 correlated with CCL17, but not CCL22, in gene expression profiling studies of FL biopsies, whereas CD40L correlated with both CCL17 and CCL22. Tumor supernatants induced preferential migration of Tregs and IL-4–producing T cells rather than IFN-γ–producing T cells, and Abs to CCR4 significantly abrogated the migration of Tregs. Our results suggest that through two distinct mechanisms, intratumoral TFH induce production of CCL17 and CCL22 by FL tumor cells and facilitate active recruitment of Tregs and IL-4–producing T cells, which, in turn, may stimulate more chemokine production in a feed-forward cycle. Thus, TFH appear to play a major role in generating an immunosuppressive tumor microenvironment that promotes immune escape and tumor survival and growth. Our results provide novel insights into the cross talk among TFH, tumor cells, and Tregs in FL, and offer potential targets for development of therapeutic strategies to overcome immune evasion.
Reciprocal Negative Cross-Talk between Liver X Receptors (LXRs) and STAT1: Effects on IFN-γ–Induced Inflammatory Responses and LXR-Dependent Gene Expression
Authors: Pascual-García Mónica , Rué Laura , León Theresa , Julve Josep , Carbó José María , Matalonga Jonathan , Auer Herbert , Celada Antonio , Escolà-Gil Joan Carles , Steffensen Knut R. , Pérez-Navarro Esther , Valledor Annabel F. 
Published: May 2013

Abstract
Liver X receptors (LXRs) exert key functions in lipid homeostasis and in control of inflammation. In this study we have explored the impact of LXR activation on the macrophage response to the endogenous inflammatory cytokine IFN-γ. Transcriptional profiling studies demonstrate that ∼38% of the IFN-γ–induced transcriptional response is repressed by LXR activation in macrophages. LXRs also mediated inhibitory effects on selected IFN-γ–induced genes in primary microglia and in a model of IFN-γ–induced neuroinflammation in vivo. LXR activation resulted in reduced STAT1 recruitment to the promoters tested in this study without affecting STAT1 phosphorylation. A closer look into the mechanism revealed that SUMOylation of LXRs, but not the presence of nuclear receptor corepressor 1, was required for repression of the NO synthase 2 promoter. We have also analyzed whether IFN-γ signaling exerts reciprocal effects on LXR targets. Treatment with IFN-γ inhibited, in a STAT1-dependent manner, the LXR-dependent upregulation of selective targets, including ATP-binding cassette A1 (ABCA1) and sterol response element binding protein 1c. Downregulation of ABCA1 expression correlated with decreased cholesterol efflux to apolipoprotein A1 in macrophages stimulated with IFN-γ. The inhibitory effects of IFN-γ on LXR signaling did not involve reduced binding of LXR/retinoid X receptor heterodimers to target gene promoters. However, overexpression of the coactivator CREB-binding protein/p300 reduced the inhibitory actions of IFN-γ on the Abca1 promoter, suggesting that competition for CREB-binding protein may contribute to STAT1-dependent downregulation of LXR targets. The results from this study suggest an important level of bidirectional negative cross-talk between IFN-γ/STAT1 and LXRs with implications both in the control of IFN-γ–mediated immune responses and in the regulation of lipid metabolism.
Inhibition of Glycogen Synthase Kinase-3 Increases NKG2D Ligand MICA Expression and Sensitivity to NK Cell–Mediated Cytotoxicity in Multiple Myeloma Cells: Role of STAT3
Authors: Fionda Cinzia , Malgarini Giulia , Soriani Alessandra , Zingoni Alessandra , Cecere Francesca , Iannitto Maria Luisa , Ricciardi Maria Rosaria , Federico Vincenzo , Petrucci Maria Teresa , Santoni Angela , Cippitelli Marco 
Published: May 2013

Abstract
Engagement of NKG2D and DNAX accessory molecule-1 (DNAM-1) receptors on lymphocytes plays an important role for anticancer response and represents an interesting therapeutic target for pharmacological modulation. In this study, we investigated the effect of inhibitors targeting the glycogen synthase kinase-3 (GSK3) on the expression of NKG2D and DNAM-1 ligands in multiple myeloma (MM) cells. GSK3 is a pleiotropic serine–threonine kinase point of convergence of numerous cell-signaling pathways, able to regulate the proliferation and survival of cancer cells, including MM. We found that inhibition of GSK3 upregulates both MICA protein surface and mRNA expression in MM cells, with little or no effects on the basal expression of the MICB and DNAM-1 ligand poliovirus receptor/CD155. Moreover, exposure to GSK3 inhibitors renders myeloma cells more efficient to activate NK cell degranulation and to enhance the ability of myeloma cells to trigger NK cell–mediated cytotoxicity. We could exclude that increased expression of β-catenin or activation of the heat shock factor-1 (transcription factors inhibited by active GSK3) is involved in the upregulation of MICA expression, by using RNA interference or viral transduction of constitutive active forms. On the contrary, inhibition of GSK3 correlated with a downregulation of STAT3 activation, a negative regulator of MICA transcription. Both Tyr705 phosphorylation and binding of STAT3 on MICA promoter are reduced by GSK3 inhibitors; in addition, overexpression of a constitutively active form of STAT3 significantly inhibits MICA upregulation. Thus, we provide evidence that regulation of the NKG2D-ligand MICA expression may represent an additional immune-mediated mechanism supporting the antimyeloma activity of GSK3 inhibitors.