bluuebunny/crossref_metadata_2025_split huggingface dataset:
N-linked glycosylation plays a crucial role in the secretion of HMGB1
Authors: Kim Young Hun , Kwak Man Sup , Park Jun Bae , Lee Shin-Ae , Choi Ji Eun , Cho Hyun-Soo , Shin Jeon-Soo 
Published: November 2015

ABSTRACT
HMGB1 protein is a delayed mediator of sepsis that is secreted to the extracellular milieu in response to various stimulants, inducing a pro-inflammatory response. HMGB1 is devoid of an endoplasmic reticulum (ER)-targeting signal peptide; hence, the mechanism of extracellular secretion is not completely understood, although HMGB1 is secreted after being subjected to post-translational modifications. Here, we identified the role of N-glycosylation of HMGB1 in extracellular secretion. We found two consensus (N37 and N134) and one non-consensus (N135) residues that were N-glycosylated in HMGB1 by performing liquid chromatography tandem mass spectrometry (LC-MS/MS) and analyzing for N-glycan composition and structure. Inhibition of N-glycosylation with tunicamycin resulted in a molecular shift of HMGB1 as assessed by gel electrophoresis. Non-glycosylated double mutant (N→Q) HMGB1 proteins (HMGB1N37Q/N134Q and HMGB1N37Q/N135Q) showed localization to the nuclei, strong binding to DNA, weak binding to the nuclear export protein CRM1 and rapid degradation by ubiquitylation. These mutant proteins had reduced secretion even after acetylation, phosphorylation, oxidation and exposure to pro-inflammatory stimuli. Taken together, we propose that HMGB1 is N-glycosylated, and that this is important for its DNA interaction and is a prerequisite for its nucleocytoplasmic transport and extracellular secretion.
The Ccl1–Kin28 kinase complex regulates autophagy under nitrogen starvation
Authors: Zhu Jing , Deng Shuangsheng , Lu Puzhong , Bu Wenting , Li Tian , Yu Li , Xie Zhiping 
Published: November 2015

ABSTRACT
Starvation triggers global alterations in the synthesis and turnover of proteins. Under such conditions, the recycling of essential nutrients by using autophagy is indispensable for survival. By screening known kinases in the yeast genome, we newly identified a regulator of autophagy, the Ccl1–Kin28 kinase complex (the equivalent of the mammalian cyclin-H–Cdk7 complex), which is known to play key roles in RNA-polymerase-II-mediated transcription. We show that inactivation of Ccl1 caused complete block of autophagy. Interestingly, Ccl1 itself was subject to proteasomal degradation, limiting the level of autophagy during prolonged starvation. We present further evidence that the Ccl1–Kin28 complex regulates the expression of Atg29 and Atg31, which is crucial in the assembly of the Atg1 kinase complex. The identification of this previously unknown regulatory pathway sheds new light on the complex signaling network that governs autophagy activity.
Reciprocal regulation of alternative lineages by Rgs18 and its transcriptional repressor Gfi1b
Authors: Sengupta Ananya , Upadhyay Ghanshyam , Sen Sayani , Saleque Shireen 
Published: November 2015

ABSTRACT
Appropriate diversification of cellular lineages from multi-potent progenitors is essential for normal development and homeostasis. The specification of erythroid and megakaryocytic lineages represents an especially vital developmental event whose molecular regulation remains incompletely defined. We now demonstrate the role of Rgs18, a GTPase-activating protein and transcriptional target of the repressor Gfi1b, in regulating these processes in mouse and human cells. Gfi1b stringently represses Rgs18 expression in erythroid cells, whereas, during megakaryocytic differentiation, declining Gfi1b levels facilitate a robust induction of Rgs18. Concordantly, alterations in Rgs18 expression produce disparate outcomes by augmenting megakaryocytic and potently suppressing erythroid differentiation and vice versa. These phenotypes reflect the differential impact of Rgs18 on signaling through p38 MAPK family proteins, and ERK1 and ERK2 (also known as MAPK3 and MAPK1, respectively) in the two lineages, which in turn alter the balance between the mutually antagonistic transcription factors Fli1 and Klf1. Overall, these results identify Rgs18 as a new and crucial effector of Gfi1b that regulates downstream signaling and gene expression programs to orchestrate erythro-megakaryocytic lineage choices. This dual role of Rgs18 in reciprocally regulating divergent lineages could exemplify generic mechanisms characteristic of multiple family members in different contexts.
BAR-SH3 sorting nexins are conserved interacting proteins of Nervous wreck that organize synapses and promote neurotransmission
Authors: Ukken Fiona P. , Bruckner Joseph J. , Weir Kurt L. , Hope Sarah J. , Sison Samantha L. , Birschbach Ryan M. , Hicks Lawrence , Taylor Kendra L. , Dent Erik W. , Gonsalvez Graydon B. , O'Connor-Giles Kate M. 
Published: November 2015

ABSTRACT
Nervous wreck (Nwk) is a conserved F-BAR protein that attenuates synaptic growth and promotes synaptic function in Drosophila. In an effort to understand how Nwk carries out its dual roles, we isolated interacting proteins using mass spectrometry. We report a conserved interaction between Nwk proteins and BAR-SH3 sorting nexins, a family of membrane-binding proteins implicated in diverse intracellular trafficking processes. In mammalian cells, BAR-SH3 sorting nexins induce plasma membrane tubules that localize NWK2, consistent with a possible functional interaction during the early stages of endocytic trafficking. To study the role of BAR-SH3 sorting nexins in vivo, we took advantage of the lack of genetic redundancy in Drosophila and employed CRISPR-based genome engineering to generate null and endogenously tagged alleles of SH3PX1. SH3PX1 localizes to neuromuscular junctions where it regulates synaptic ultrastructure, but not synapse number. Consistently, neurotransmitter release was significantly diminished in SH3PX1 mutants. Double-mutant and tissue-specific-rescue experiments indicate that SH3PX1 promotes neurotransmitter release presynaptically, at least in part through functional interactions with Nwk, and might act to distinguish the roles of Nwk in regulating synaptic growth and function.
Fabrication of Self-Organized, Two-Dimensional Diluted Magnetic Semiconductors Based on Organic–Inorganic Layered Perovskite-Type Compounds
Authors: Kubota Kohei , Koshimizu Masanori , Saito Hirotoshi , Asai Keisuke 
Published: November 2015

Abstract
We have fabricated diluted magnetic semiconductors (DMSs) based on organic–inorganic layered perovskite-type compounds, in which multiple quantum well structures were formed. DMSs were synthesized by substituting a small fraction of the Pb2+ ions in the inorganic layers with Mn2+ or Cu2+. XRD, ESR, and magnetization results indicate that the samples form mixed crystals. The Mn-doped samples exhibited paramagnetism down to 10 K, whereas the Cu-doped samples exhibited ferromagnetism at temperatures lower than ca. 15 K. In addition, a clear exciton peak was observed in the absorption spectrum of each sample. These results indicate that self-organized DMS quantum wells were successfully fabricated.
Isolation and Structural Characterization of a Metallacyclic Compound by Selective Protection of a Single CO Ligand in a Ruthenium Complex
Authors: Oyama Dai , Hamada Takashi , Ukawa Narumi , Mochizuki Ryuta , Takase Tsugiko 
Published: November 2015

Abstract
The metallacyclic compound was isolated by chemical reduction after selective protection of a single CO ligand in a dicarbonylruthenium(II) complex bearing the redox-active bidentate pyridyl ligand, 2-(2-pyridyl)-1,8-naphthyridine. Spectroscopic and X-ray structural analyses of the metallacycle revealed hydrogenation of the naphthyridine unit.
Simple Preparation and Characterization of Viscoelastic Gels Induced by Multiple Intermolecular Interactions Using Low-Molecular-Weight Species
Authors: Kubo Takuya , Kuroda Kenta , Naito Toyohiro , Mukai Sada-atsu , Sano Tomoharu , Akiyoshi Kazunari , Otsuka Koji 
Published: November 2015

Abstract
We report simple preparation of viscoelastic gels induced by multiple intermolecular interactions using low-molecular-weight aromatic sulfonates and alkylammoniums in water. The flow properties of the gels could be controlled between viscoelastic gel to high-viscosity liquid by changing the number of sulfonates and the length of alkyl chain among the components. Rheology evaluations of these viscoelastic materials showed significant differences of G′ and G′′ depending on the components. Also, spin–lattice relaxation, T1 in swollen-resin magic angle spinning 13C NMR well supported the differences of the viscoelastic gels in “hardness” according to the strength of hydrophobic interactions and π–π interactions contributed to the hydrogelation by alkyl chains and aromatic rings, respectively. Furthermore, we demonstrated a possibility for self-healing of a viscoelastic gel prepared with sodium 1,3,6-naphthalene trisulfonate and hexadecyltrimethylammonium bromide.
Identifying and Structuring the Objectives of the Islamic State of Iraq and the Levant (ISIL) and Its Followers
Authors: Siebert Johannes , von Winterfeldt Detlof , John Richard S. 
Published: November 2015

This study addresses three questions: What are the objectives of the leaders of ISIL? What are the objectives of the followers of ISIL? How are the two sets of objectives related? To answer these questions, we analyzed the transcripts of interviews and presentations of 59 subject matter experts (SMEs) and conducted a separate analysis of speeches of ISIL leaders and selected Internet sources. In both efforts we identified and structured the strategic, fundamental, and means objectives of ISIL and its followers. The results indicate that ISIL’s leaders pursue four strategic objectives: (1) Establish a Caliphate in Iraq and the Levant, (2) Control and Govern the Caliphate, (3) Expand Islam and Sharia Law Worldwide, and (4) Recreate the Power and Glory of (Sunni) Islam. The followers’ objectives can be partitioned into three strategic objectives: Humanitarian Fulfillment, Religious Fulfillment, and Personal Fulfillment. The objectives identified from the SME interviews were similar to those identified from ISIL leaders’ statements and the Internet. However, the Internet search revealed many more personal objectives of ISIL followers. The results further indicate that ISIL’s leadership objectives are closely aligned with those of its followers. There also is a sharp contrast between the objectives of ISIL and those of Al Qaeda, particularly ISIL’s emphasis on occupying and controlling territories in Iraq and Syria versus Al Qaeda’s focus on worldwide jihad.
Constrained Resource Assignments: Fast Algorithms and Applications in Wireless Networks
Authors: Berger André , Gross James , Harks Tobias , Tenbusch Simon 
Published: November 2015

Resource assignment problems occur in a vast variety of applications, from scheduling problems over image recognition to communication networks. Often these problems can be modeled by a maximum weight matching problem in (bipartite) graphs or generalizations thereof, and efficient and practical algorithms are known for these problems. Although in some of the applications an assignment of the resources may be needed only once, in many of these applications, the assignment has to be computed more often for different scenarios. In that case it is often essential that the assignments can be computed very fast. Moreover, implementing different assignments in different scenarios may come with a certain cost for the reconfiguration of the system. In this paper, we consider the problem of determining optimal assignments sequentially over a given time horizon, where consecutive assignments are coupled by constraints that control the cost of reconfiguration. We develop fast approximation and online algorithms for this problem with provable approximation guarantees and competitive ratios. Moreover, we present an extensive computational study about the applicability of our model and our algorithms in the context of orthogonal frequency division multiple access (OFDMA) wireless networks, finding a significant performance improvement for the total bandwidth of the system using our algorithms. For this application (the downlink of an OFDMA wireless cell) , the run time of matching algorithms is extremely important, having an acceptable range of a few milliseconds only. For the considered realistic instances, our algorithms perform extremely well: the solution quality is, on average, within a factor of 0.8–0.9 of optimal off-line solutions, and the running times are at most 5 ms per phase even in the worst case. Thus, our algorithms are well suited to be applied in the context of OFDMA systems. Data, as supplemental material, are available at http://dx.doi.org/10.1287/mnsc.2015.2221 . This paper was accepted by Teck-Hua Ho, optimization.
The relationship between attitudes towards menarche and current attitudes towards menstruation of women: A comparative study
Authors: Erbil Nülüfer , Felek Neslihan , Karakaşlı Ebru 
Published: November 2015

<p><strong>Purpose:</strong> The study was conducted to determine affecting factors and to investigate relationship between attitudes towards menarche and current attitudes towards menstruation of women and to compare characteristics of menarche and menstruation of women who were born and raised in the northeastern and western of Turkey. </p><p><strong>Method and Materials:</strong> This descriptive and comparative study was participated totally 400 volunteer women.<strong> </strong>The data were collected using a questionnaire containing defined closed and open-ended questions.</p><p><strong>Results: </strong>The study results showed that women living in Ordu province than women living in Aydın province expressed positive attitudes toward menarche, and the difference was found significant. Nevertheless, attitudes towards current menstruation were similar in both provinces. When the data of 400 women were evaluated together; women’s attitudes towards menarche were compared with the current attitudes towards menstruation, and the difference was found statistically significant. Although attitudes of the women towards menarche varied according to where they lived, their current attitudes towards menstruation were similar. In addition, depending upon the province, there were statistically significant differences in women’s age at menarche, recollection of menarche, and feelings of shame or happiness at menarche, source and adequacy of information about menstruation, and regularity of their menstrual cycle. </p><p><strong>Conclusions:</strong> Early menstrual experiences may be related to menstrual experiences later in life and women’s attitudes towards menstruation were not affected by geographical locations. In order to develop positive attitudes towards menarche and a woman’s future years of menstrual cycles, it is very important that accurate and adequate information for young girls be provided before menarche. </p>
Avaliação da influência da adição de fibras de aço no concreto fluido solicitado por tensões de tração
Authors: Góis Fernanda Alves Pinto , Barboza Aline Da Silva Ramos 
Published: November 2015

<div><p class="SPabstract">O desenvolvimento de novas tecnologias na produção do concreto ao longo do tempo vêm permitindo elevar a sua resistência à compressão, melhorar a trabalhabilidade e garantir maior durabilidade. Apesar de suas muitas vantagens e melhorias atingidas, o concreto apresenta limitações relacionadas ao seu comportamento frágil quando na presença de esforços de tração. Uma alternativa para contornar este problema é a utilização de armadura dispersa por meio de fibras no concreto, obtendo-se assim um material compósito que possui características diferentes do material original. Buscando colaborar na avaliação da eficiência da adição de fibras, o presente trabalho analisa o comportamento de concretos fluidos reforçados com fibras de aço, submetidos a tensões de tração, considerando como parâmetros de influência o fator de forma e a fração volumétrica. Para isso, foram utilizados corpos-de-prova cilíndricos de dimensões de 10 cm x 20 cm de concreto fluido de resistência usual, com teor de fibras de aço de 0,4% e 0,8% da quantidade de cimento em massa e com fatores de forma de 33, 50 e 80. Os corpos de prova foram submetidos a ensaios de tração por compressão diametral. Com os resultados obtidos foi possível afirmar que a matriz cimentícia fluida, com adição de fibras com maior fator de forma e maior fração volumétrica tendem a ser mais eficientes na presença de esforços de tração.</p></div>
Stress Testing International Financial Reporting Standards (IFRS): Accounting for Stability and the Public Good in a Financialized World
Authors: Haslam Colin , Tsitsianis Nick , Hoinaru Razvan , Andersson Tord , Katechos George 
Published: November 2015

AbstractThe recent Maystadt report (2013) challenged the European Parliament to modify governance arrangements surrounding the design and endorsement of international financial reporting standards (IFRS) issued by the International Accounting Standards Board (IASB). In addition the Maystadt report constructs an argument that accounting information has the capacity to also modify behaviour and that this might not be conducive for the European public good, financial stability and economic development. In this paper we argue that IFRS need to be stress tested for their impact on firm-level financial stability in a financialized world. The financialized firm can revalue a range of assets to their market value crystalizing future earnings into current values but these valuations can become impaired. Asset value impairments will be charged to shareholder equity but this is being hollowed out because a higher proportion of earnings are being distributed to shareholders. Accounting disclosures are not only an information feed to users they inform the stewardship and control of a firm’s resources and in the financialized firm the potential for financial instability is heightened and this can translate into a moral hazard for society.
“Why Polarized Trust Matters”
Authors: Hetherington Marc 
Published: November 2015

AbstractThe early 2000s has witnessed the rise of a new phenomenon in public opinion, polarized political trust. By polarized, I mean that those who identify with the party opposite the president express much less trust in government than those who identify with the president’s party. Indeed, outparty partisans express almost no political trust at all. This is important because it is this group of people who have traditionally been the bridge that allows for consensus building in the electorate. Consensus in the electorate, in turn, encourages cooperation across party lines in Congress. Without consensus but with deep distrust of the other party, office holders from the party opposite the president have no incentive to rise above their worst partisan instincts because partisans from their side will tend to blame the president’s party for resultant problems of governance.
Self-concept and social comparison and their relation with smoking and alcohol consumption in adolescents
Authors: Özdemir Aysel , Koçoğlu Gülay 
Published: November 2015

Abstract

Background:
Some attitudes that possess certain risks such as smoking and alcohol consumption rate are increasing among the adolescent age group. For this reason it is very important to shape the attitudes of adolescents during their growth period.

Objective:
The aim of this study is to investigate the association of social comparison and self-concept of adolescents’ relation with smoking and alcohol consumption.

Subjects:
Study was conducted as a prospective study in nine high schools which are located in city of Bursa, Turkey. Nine hundred and fifty-three (n=953) students were included.

Methods:
Data were gathered using a questionnaire form especially developed by researchers for this study according to relevant literature. For social comparison and self-concept evaluation Piers-Harris Self Concept Scale and Social Comparison Scale of Gilbert were used.

Results:
The mean age was 15.74±1.27 and 411 of cases (43.1%) were male. It was determined that smoking and alcohol consumption increased as the age of adolescents’ age increases. Rate of smoking and alcohol consumption was significantly higher in males compared to females. The mean self-concept scale score of the cases who smoke (52.30±11.01) were found to be lower than the non-smokers (56.07±10.13). The mean social comparison scale score of smoking adolescents’ (70.25±23.99) was higher than the non-smokers (69.43±25.47).

Conclusion:
The social comparison scale scores were found to be higher in adolescents who consume alcohol and smoke tobacco. In contrast to this result self-concept scale scores were low. As a conclusion this study reveals that attitudes such as smoking and alcohol consumption are mostly influenced by self-concept of the adolescents and family attitudes towards adolescents.
Parental alienation: the impact on men’s mental health
Authors: Sher Leo 
Published: November 2015

Abstract
Parental alienation is defined as a mental state in which a child, usually one whose parents are engaged in a high-conflict separation or divorce, allies himself strongly with one parent (the preferred parent) and rejects a relationship with the other parent (the alienated parent) without legitimate justification. Parental alienation may affect men’s mental health: a) parental alienation negatively influences mental health of male children and adolescents who are victims of parental alienation. Alienated children/adolescents display guilt, sadness, and depressed mood; low self-esteem and lack of self-confidence; distress and frustration; lack of impulse control, substance abuse and delinquent behavior; separation anxiety, fears and phobias; hypochondria and increased tendency to develop psychosomatic illness; suicidal ideation and suicide attempt; sleep and eating disorders; educational problems; enuresis and encopresis; b) parental alienation negatively affects the mental health of adult men who were victims of parental alienation when they were children and/or adolescents. Long-term effects of parental alienation include low self-esteem, depression, drug/alcohol abuse, lack of trust, alienation from own children, divorce, problems with identity and not having a sense of belonging or roots, choosing not to have children to avoid being rejected by them, low achievement, anger and bitterness over the time lost with the alienated parent; c) parental alienation negatively influences mental health of men who are alienated from their children. Fathers who have lost some or all contact with their children for months or years following separation or divorce may be depressed and suicidal.
A Semiparametric Bayesian Approach for Analyzing Longitudinal Data from Multiple Related Groups
Authors: Das Kiranmoy , Afriyie Prince , Spirko Lauren 
Published: November 2015

AbstractOften the biological and/or clinical experiments result in longitudinal data from multiple related groups. The analysis of such data is quite challenging due to the fact that groups might have shared information on the mean and/or covariance functions. In this article, we consider a Bayesian semiparametric approach of modeling the mean trajectories for longitudinal response coming from multiple related groups. We consider matrix stick-breaking process priors on the group mean parameters which allows information sharing on the mean trajectories across the groups. Simulation studies are performed to demonstrate the effectiveness of the proposed approach compared to the more traditional approaches. We analyze data from a one-year follow-up of nutrition education for hypercholesterolemic children with three different treatments where the children are from different age-groups. Our analysis provides more clinically useful information than the previous analysis of the same dataset. The proposed approach will be a very powerful tool for analyzing data from clinical trials and other medical experiments.