bluuebunny/crossref_metadata_2025_split huggingface dataset:
RETRACTION: KLF8 is required for bladder cancer cell proliferation and migration
Authors: Liang Kai , Liu Tao , Chu Ning , Kang Jian , Zhang Rui , Yu Yong , Li Dongqi , Lu Dexiang 
Published: October 2014

AbstractRETRACTION: Krüppel‐like factor 8 (KLF8) belongs to the Sp/KLF family of transcription factors. Recently, it is affirmed that KLF8 plays an important role in the regulation of epithelial–mesenchymal transition, which is a key process that occurs during cancer metastasis. Although the overexpression of KLF8 has been observed in several types of human cancers, the functional role of KLF8 in human bladder cancer remains unknown. Here, we investigated the effects of KLF8 knockdown on bladder cancer cell proliferation and migration in vitro. Lentivirus‐mediated small interfering RNA (siRNA) targeting KLF8 specifically downregulated its expression in T24 and BT5637 bladder cancer cells. Knockdown of KLF8 significantly inhibit cell proliferation and colony formation. Cell cycle analysis showed that knockdown of KLF8 arrested T24 cells in the G0/G1 phase. Moreover, cell migration was attenuated in T24 cells after KLF8 knockdown. Furthermore, knockdown of KLF8 resulted in a reduction in vimentin and N‐cadherin expression and an increase in β‐catenin expression. These results indicate that KLF8 plays a crucial role in proliferation and migration of bladder cancer cells, and inhibition of KLF8 by siRNA may provide a potential therapeutic approach for gene therapy in bladder cancer.
Determination of a novel nonfluorinated quinolone, nemonoxacin, in human feces and its glucuronide conjugate in human urine and feces by high‐performance liquid chromatography–triple quadrupole mass spectrometry
Authors: He Gaoli , Guo Beining , Yu Jicheng , Zhang Jing , Wu Xiaojie , Cao Guoying , Shi Yaoguo , Tsai Cheng‐yuan 
Published: October 2014

AbstractThree methods were developed and validated for determination of nemonoxacin in human feces and its major metabolite, nemonoxacin acyl‐β‐ d‐glucuronide, in human urine and feces. Nemonoxacin was extracted by liquid–liquid extraction in feces homogenate samples and nemonoxacin acyl‐β‐ d‐glucuronide by a solid‐phase extraction procedure for pretreatment of both urine and feces homogenate sample. Separation was performed on a C18 reversed‐phase column under isocratic elution with the mobile phase consisting of acetonitrile and 0.1% formic acid. Both analytes were determined by liquid chromatography–tandem mass spectrometry with positive electrospray ionization in selected reaction monitoring mode and gatifloxacin as the internal standard. The lower limit of quantitation (LLOQ) of nemonoxacin in feces was 0.12 µg/g and the calibration curve was linear in the concentration range of 0.12–48.00 µg/g. The LLOQ of the metabolite was 0.0010 µg/mL and 0.03 µg/g in urine and feces matrices, while the linear range was 0.0010–0.2000 µg/mL and 0.03–3.00 µg/g, respectively. Validation included selectivity, accuracy, precision, linearity, recovery, matrix effect, carryover, dilution integrity and stability, indicating that the methods can quantify the corresponding analytes with excellent reliability. The validated methods were successfully applied to an absolute bioavailability clinical study of nemonoxacin malate capsule. Copyright © 2014 John Wiley & Sons, Ltd.
Correlations between brain structures and study time at home in healthy children: a longitudinal analysis
Authors: Asano Michiko , Taki Yasuyuki , Hashizume Hiroshi , Takeuchi Hikaru , Thyreau Benjamin , Sassa Yuko , Asano Kohei , Kawashima Ryuta 
Published: October 2014

AbstractIntroductionLike sleeping and eating habits, the study habits adopted by children when they are at home are important contributors to lifestyle and they affect cognitive ability. It has recently been reported that sleeping and eating habits change the brain structure of children. However, no research on the effect of study habits at home on the brain structure of children has been conducted thus far. We investigated the effects of study habits at home on the brain structures of healthy children by examining correlations between study time at home and changes in brain structure over the course of 3 years.MethodsWe used the brain magnetic resonance images of 229 healthy children aged 5.6–18.4 years and computed the changes (time 2–time 1) in regional gray matter and white matter volume (rWMV) using voxel‐based morphometry. Whole‐brain multiple regression analysis revealed a significant positive correlation between study time at home and changes in rWMV in the right superior frontal gyrus (SFG). Behaviorally, we found a significant positive correlation between study time at home and change in the verbal comprehension index (VCI), one of the subscales of the Wechsler Intelligence Scale for Children–third edition (WISC–III).Results and ConclusionsGiven that the SFG is involved in memory control and that the VCI measures abilities related to vocabulary, our results indicate that greater SFG involvement in the memorization component of longer study times may result in greater increases in the number of axons and more axon branching and myelination, causing plastic changes in the neural network involved in memory processes.
Downregulation of NDUFB6 due to 9p24.1‐p13.3 loss is implicated in metastatic clear cell renal cell carcinoma
Authors: Narimatsu Takahiro , Matsuura Keiko , Nakada Chisato , Tsukamoto Yoshiyuki , Hijiya Naoki , Kai Tomoki , Inoue Toru , Uchida Tomohisa , Nomura Takeo , Sato Fuminori , Seto Masao , Takeuchi Ichiro , Mimata Hiromitsu , Moriyama Masatsugu 
Published: October 2014

AbstractThis study was conducted to clarify the genomic profiles of metastatic clear cell renal cell carcinomas (ccRCCs) and identify the genes responsible for development of metastasis. We analyzed the genomic profiles of 20 cases of primary ccRCC and their corresponding metastases using array‐based comparative genomic hybridization, and identified 32 chromosomal regions in which gene copy number alterations were detected more frequently in metastases than in the primary tumors. Among these 32 regions, 9p24.1‐p13.3 loss was the most statistically significant alteration. Furthermore, we found that patients with 9p24.1‐p13.3 loss in primary tumors exhibited significantly lower rates of recurrence‐free and cancer‐specific survival, suggesting that 9p loss in the primary tumor is a potential biomarker predicting early recurrence of metastasis. Interestingly, the genomic profiles of primary tumors with 9p loss resembled those of their corresponding metastases, though 9p loss was accumulated in the metastases derived from the primary tumors without 9p loss. Comparison of the mRNA expression levels revealed that 2 of 58 genes located at 9p24.1‐p13.3 were downregulated due to gene copy number loss in ccRCCs. An overexpression study of these two genes in ccRCC cell lines revealed that downregulation of NDUFB6 due to loss at 9p24.1‐p13.3 may confer a growth advantage on metastatic ccRCC cells. These results were confirmed by analyzing the data of 405 cases of ccRCC obtained from The Cancer Genome Atlas (TCGA). On the basis of our present data, we propose that NDUFB6 is a possible tumor suppressor of metastatic ccRCCs.
Immunohistochemical mapping of neuropeptide Y in the tree shrew brain
Authors: Ni Rong‐Jun , Shu Yu‐Mian , Luo Peng‐Hao , Fang Hui , Wang Yu , Yao Lei , Zhou Jiang‐Ning 
Published: October 2014

ABSTRACTDay‐active tree shrews are promising animals as research models for a variety of human disorders. Neuropeptide Y (NPY) modulates many behaviors in vertebrates. Here we examined the distribution of NPY in the brain of tree shrews (Tupaia belangeri chinensis) using immunohistochemical techniques. The differential distribution of NPY‐immunoreactive (‐ir) cells and fibers were observed in the rhinencephalon, telencephalon, diencephalon, mesencephalon, metencephalon, and myelencephalon of tree shrews. Most NPY‐ir cells were multipolar or bipolar in shape with triangular, fusiform, and/or globular perikarya. The densest cluster of NPY‐ir cells were found in the mitral cell layer of the main olfactory bulb (MOB), arcuate nucleus of the hypothalamus, and pretectal nucleus of the thalamus. The MOB presented a unique pattern of NPY immunoreactivity. Laminar distribution of NPY‐ir cells was observed in the MOB, neocortex, and hippocampus. Compared to rats, the tree shrews exhibited a particularly robust and widespread distribution of NPY‐ir cells in the MOB, bed nucleus of the stria terminalis, and amygdala as well as the ventral lateral geniculate nucleus and pretectal nucleus of the thalamus. By contrast, a low density of neurons were scattered in the striatum, neocortex, polymorph cell layer of the dentate gyrus, superior colliculus, inferior colliculus, and dorsal tegmental nucleus. These findings provide the first detailed mapping of NPY immunoreactivity in the tree shrew brain and demonstrate species differences in the distribution of this neuropeptide, providing an anatomical basis for the participation of the NPY system in the regulation of numerous physiological and behavioral processes. J. Comp. Neurol. 523:495–529, 2015. © 2014 Wiley Periodicals, Inc.
Digital in vivo 3D atlas of the antennal lobe of Drosophila melanogaster
Authors: Grabe Veit , Strutz Antonia , Baschwitz Amelie , Hansson Bill S. , Sachse Silke 
Published: October 2014

ABSTRACTAs a model for primary olfactory perception, the antennal lobe (AL) of Drosophila melanogaster is among the most thoroughly investigated and well‐understood neuronal structures. Most studies investigating the functional properties and neuronal wiring of the AL are conducted in vivo, although so far the AL morphology has been mainly analyzed in vitro. Identifying the morphological subunits of the AL—the olfactory glomeruli—is usually done using in vitro AL atlases. However, the dissection and fixation procedure causes not only strong volumetric but also geometrical modifications; the result is unpredictable dislocation and a distortion of the AL glomeruli between the in vitro and in vivo brains. Hence, to characterize these artifacts, which are caused by in vitro processing, and to reliably identify glomeruli for in vivo applications, we generated a transgenic fly that expresses the red fluorescent protein DsRed directly fused to the presynaptic protein n‐synaptobrevin, under the control of the pan‐neuronal promotor elav to label the neuropil in the live animal. Using this fly line, we generated a digital 3D atlas of the live Drosophila AL; this atlas, the first of its kind, provides an excellent geometric match for in vivo studies. We verified the identity of 63% of AL glomeruli by mapping the projections of 34 GAL4‐lines of individual chemosensory receptor genes. Moreover, we characterized the innervation patterns of the two most frequently used GAL4‐lines in olfactory research: Orco‐ and GH146‐GAL4. The new in vivo AL atlas will be accessible online to the neuroscience community. J. Comp. Neurol. 523:530–544, 2015. © 2014 Wiley Periodicals, Inc.
Efficient Learning for Test Feature Classifier by Overlap Index List
Authors: Matsuo Yoshikazu , Takauji Hidenori , Kaneko Shun'Ichi 
Published: October 2014

SUMMARYThis paper presents a novel low cost learning algorithm for a test feature classifier by use of an overlap index list (OIL). In general, classifiers need a large amount of training data to achieve the high performance, which results in long computation times. The proposed algorithm using OIL can maintain search and check elemental combinatorial features from lower dimensions up to higher ones. Classification problems in real industrial inspection lines have been solved by the proposed algorithm, and large reductions in computation time have been obtained.
On‐Chip Manipulation of Magnetic Beads for Integrated Immunosensor
Authors: Ishikawa Tomohiro , Lee Jaesung , Miyake Ryo 
Published: October 2014

SUMMARYThe purpose of this work is in‐plane manipulation of dispersed magnetic beads on a chip. This makes an immunoassay chip that completes all its procedures on itself and eliminates any off‐chip components. A test chip was drawn in a 180 nm standard complementary metal oxide semiconductor process and tested. Beads with a diameter of 2.8 μm were diluted and applied to the chip. The chip successfully manipulated them with an affordable current down to 5 mA.
Fundamental Study of Physiological Evaluation by Paired Comparison Test and Heart Rate Variability: The Effect of Chromatic Lights on Living Organisms
Authors: Nomoto Yohei , Ohmuta Takao , Ohya Tetsuya , Sawai Kei , Koyama Hironori , Kawasumi Masashi 
Published: October 2014

SUMMARYThe popularization of LEDs and the development of light control technology have provided chromatic lights such as red, blue, and green. Chromatic lights are attracting attention in connection with the psychology and physiological effects of color. It is predicted that chromatic lights will result in favorable innovations in life spaces. This investigation studied chromatic lights as related to heart rate variability (HRV), using a psychological methodology, that is, the paired comparison test. The subjects of the paired comparison test were 14 healthy participants (age 21.9 ±3.7 years). The HRV subjects were seven healthy participants (age 24.0 ± 3.9 years). We examined the experimental results from two viewpoints. (1) In the results of the paired comparison test, the scores for white‐ice, yellow, and green were higher than those for the other colors, and the scores for blue, red, and pink were lower than those for the other colors. (2) In the results for HRV, the mean high‐frequency (HF) values for white‐ice, yellow, and green were higher than those for the other colors, and the mean HF values for blue, red, and pink were lower than those for the other colors. It is concluded that by using physiological parameters and a questionnaire it is possible to analyze the effect of chromatic lights on living organisms.
Pro‐glacial lake sedimentation from jökulhlaups (GLOF), Blåmannsisen, northern Norway
Authors: Xu Mengzhen , Bogen Jim , Wang Zhaoyin , Bønsnes Truls E. , Gytri Stine 
Published: October 2014

AbstractOutburst floods from glacier‐dammed lakes are major events associated with glacier thinning and volume reduction. This paper investigates jökulhlaups emanating from the glacier‐dammed lake Øvre Messingmalmvatn at Rundvassbreen, an outlet glacier of the Blåmannsisen ice cap in northern Norway. Since 2001, the lake has several times been observed to drain suddenly, causing jökulhlaup outbursts into the pro‐glacial lake Rundvatnet. Varve analysis and lead‐210 (210Pb) dating were used to date sediment cores taken from Rundvatnet. It was found that sedimentation from jökulhlaups is recognizable in the lake as distinct sand layers embedded in the varved silt‐clay sequence which represents the normal lake sedimentation. Sand fractions were carried in suspension because of the extreme hydraulic conditions of jökulhlaups. The thickest sand layer was deposited during the 2001 jökulhlaup which lasted three days and had a total volume of 40 ×106 m3. Jökulhlaups were also recorded in 2005, 2007, 2009, and 2010; they each resulted in a sand layer. Annual sediment accumulation in Rundvatnet increased up to 10‐fold during the years with jökulhlaup outburst floods, from a normal value of 1–2 mm yr−1 to 8–10 mm yr−1. Five other jökulhlaups were identified from the 1910–1930 sedimentation interval, in addition to those observed in 2001–2010; there appear to have been none for 70 years during 1931–2000. Each jökulhlaup was preceded by a period when the glacier thinned to a critical volume and could no longer withstand the hydrostatic pressure of Øvre Messingmalmvatn; consequently a tunnel developed beneath the glacier, leading to a jökulhlaup. Statistical analyses of the correlations between the pro‐glacial sedimentation rate and temperature and precipitation suggested that although climate conditions are expected to influence sedimentation in the pro‐glacial catchment, a host of other interacting factors moderate the availability and delivery of sediment to the pro‐glacial system, making the processes responsible for changes in pro‐glacial sedimentation to remain uncertain. Copyright © 2014 John Wiley & Sons, Ltd.
Evolution of forest precipitation water storage measurement methods
Authors: Friesen Jan , Lundquist Jessica , Van Stan John T. 
Published: October 2014

AbstractPrecipitation intercepted by forests plays a major role in more than one‐fourth of the global land area's hydrologic cycle. Direct in situ measurement of intercepted precipitation is challenging, and thus, it is typically indirectly estimated through comparing precipitation under forest cover and in the open. We discuss/compare measurement methods for forest precipitation interception beyond classical budgeting and then recommend future directions for improving water storage estimation. Comparison of techniques shows that methods submerging tree components produce the largest water storage capacity values. Whole‐tree lysimeters have been used with great success at quantifying water storage for the integrated system yet are unable to separate trunk versus canopy storage. Remote sensing, particularly signal attenuation, may permit this separation. Mechanical displacement methods show great promise and variety of techniques: pulley/spring system, branch strain sensors, trunk compression sensors and photography. Relating wind sway to water storage also shows great promise with negligible environmental disruption yet is currently at the proof‐of‐concept stage. Suggested future directions focus on development of common features regarding all discussed methods: (i) measurement uncertainties or processes beyond interception influencing the observed signal, (ii) scaling approaches to move from single tree components to the single‐tree and forest scales and (iii) temporal scaling to estimate the relevance of single‐interception components over longer timescales. Through addressing these research needs, we hope the scientific community can develop an ‘integrated’ monitoring plan incorporating multiple measurement techniques to characterize forest‐scale water storage dynamics while simultaneously investigating underlying (smaller‐scale) components driving those dynamics across the spectrum of precipitation and forest conditions. Copyright © 2014 John Wiley & Sons, Ltd.
Observation in the Growth of Selenium Nanoparticles
Authors: Lin Zong‐Hong , Lin Fu‐Chu , Wang C. R. Chris 
Published: October 2014

AbstractA mild chemical reduction method was developed to synthesize selenium nanoparticles. The observation of the particle growth within a five‐minute time frame at an elevated temperature is achieved, and it was evidenced by analyzing the particle sizes from electron micrographs. The samples at individual growth stages were taken after the reaction was quenched by cooling to stop further particle growth. The nucleation and growth processes dominate different reaction time regimes under the experimental condition. We demonstrate the size evolution of the selenium nanoparticles from 30 nm to about 200 nm.
Polydimethylsiloxane Soft Template Assisted Synthesis of Silver Nanoparticles via Vapor‐Solid Reaction Growth
Authors: Hsia Chih‐Hao , Yen Ming‐Yu , Chiu Hsin‐Tien , Lee Chi‐Young 
Published: October 2014

AbstractA new route to synthesize silver nanoparticles via vapor‐solid reaction growth (VSRG) is reported. Employing AgCl to react with (Me3Si)4Si in a sealed tube (433–673 K) generates silver particles (diameter ≥ 10 μm). Addition of polydimethylsiloxnae (PDMS) to the reaction dramatically reduces the particle size (diameter 60–1200 nm). Moreover, formation of silver nanorods and nanocubes was discovered in some reaction conditions. Adding PDMS and varying the reaction temperature were the key factors influencing the Ag particle size.
Utility of N‐[4‐(N‐Substituted Sulfamoyl)Phenyl] Cyanothioformamides in the Synthesis of Heterocyclic Compounds
Authors: El‐Gaby Mohamed S. A. , El‐Sharief Ahmed M. Sh , Atalla Ahmed A. , El‐Adasy Abu‐Bakr A. A. M. 
Published: October 2014

AbstractThe novel cyanothioformamides 2a‐d were prepared by treatment of isothiocyanatosulfonamides 1a‐d with potassium cyanide at room temperature. Cyclocondensation of compounds 2b,c with phenyl isocyanate as electrophile furnished the corresponding imidazolidines 3a,b. The reactivity of compound 3a towards some nitrogen nucleophiles was investigated. Thus, the thiosemicarbazone 4 and imidazo[4,5‐b]quinoxaline 6 were synthesized by condensation of compound 3a with thiosemicarbazide and o‐phenylenediamine, respectively. Treatment of 3a with hydrochloric acid afforded compound 7. Our investigation was extended to include the reactivity of cyanothioformamide 2 towards o‐aminophenol, anthranilic acid, and o‐phenylenediamine and yielded the corresponding heterocycles 9, 11 and 13 derivatives, respectively. Structures of the synthesized compounds were established by their elemental analysis and spectral data.
A Novel Method for the Synthesis of Telluroesters
Authors: Li Xue , Zhang Songlin , Zhang Yongmin 
Published: October 2014

AbstractDiaryl ditellurides were conveniently reduced by a system consisting of cerium trichloride and samarium intetrahydrofuran to produce the aryltellurolates. This “living” tellurolate anion species reacted smoothly with acid chlorides and acid anhydrides to afford telluroesters in good yields under mild and neutral conditions.
Micellar Effect on the Reaction of Chromium(VI) Oxidation of L‐Sorbose in the Presence and Absence of Picolinic Acid in Aqueous Acid Media: A Kinetic Study
Authors: Saha Bidyut , Das Mahua , Mohanty Rajani K. , Das Asim K. 
Published: October 2014

AbstractThe kinetics and mechanism of chromic acid oxidation of L‐sorbose in the presence and absence of picolinic acid (PA) have been studied under the conditions, [L‐sorbose]T » [PA]T » [Cr(VI)]T, at different temperatures. In the absence of PA, the monomeric chromic acid undergoes esterification with the substrate followed by the acid catalysed redox decomposition of the Cr(VI)‐substrate ester through glycol splitting to formaldehyde and the lactone of C5‐aldonic acid and Cr(IV) which subsequently participates in the faster reactions. In the presence of PA, the Cr(VI)‐PA complex produced in a pre‐equilibrium step experiences a nucleophilic attack by the substrate to produce a ternary complex which decomposes through glycol splitting giving rise to the organic products and Cr(IV)‐PA complex. Both the uncatalysed and PA‐catalysed paths show the first‐order dependence on [L‐sorbose]T and [Cr(VI)]T. The PA‐catalysed path is first‐order in [PA]T and it shows a fractional order in [H+]. The uncatalysed path shows a second‐order dependence on [H+]. In the presence of the surfactants like N‐cetylpyridinium chloride (CPC, a cationic surfactant) and sodium dodecyl sulfate (SDS, an anionic sulfate), the reaction orders remain unchanged. CPC has been found to inhibit both the uncatalysed and PA‐catalysed paths while SDS shows the rate accelerating effect for both the uncatalysed and PA‐catalysed paths. The observed micellar effects have been rationalised by considering the distribution of the reactants between the micellar and aqueous phases in terms of the proposed reaction mechanism.
New Sesquiterpenes from Ligulariopsis Shichuana
Authors: Wang Wen‐Shu , Gao Kun , Jia Zhong‐Jian 
Published: October 2014

AbstractA thorough investigation of Ligulariopsis shichuana afforded five new sesquiterpenes, including 1β,10β‐epoxy‐3α‐angeloyloxy‐9β‐acetoxy‐8α,11β‐dihydroxybakkenolide (1), 1β,7‐dihydroxy‐3β‐acetoxy‐noreremophil‐6(7),9(10)‐dien‐8‐one (2), 8α‐hydroxy‐3‐oxoeremophil‐1(2),7(11),9(10)‐trien‐8β(12)‐olide (3), 1β,10β‐dihydroxy‐3β‐acetoxyeremophil‐7(11),8(9)‐dien‐8(12)‐olide (4) and 1β,10β‐epoxy‐8,12‐dihydroxy‐3β‐acetoxy‐9β‐angeloyloxyeremophil‐7(11)‐en‐8,12‐disemiketal (5). Their structures were established by spectroscopic methods and 2D NMR techniques. In addition, bakkenolide (1) and eremophilenolides (5) showed antibacterial and cytotoxic activities.