bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative Analyses of a Cystic Fibrosis Isolate of Bordetella bronchiseptica Reveal Differences in Important Pathogenic Phenotypes
Authors: Sukumar Neelima , Nicholson Tracy L. , Conover Matt S. , Ganguly Tridib , Deora Rajendar 
Published: January 2014

ABSTRACT

Bordetella bronchiseptica
is a Gram-negative bacterium that infects and causes disease in a wide variety of animals.
B. bronchiseptica
also infects humans, thereby demonstrating zoonotic transmission. An extensive characterization of human
B. bronchiseptica
isolates is needed to better understand the distinct genetic and phenotypic traits associated with these zoonotic transmission events. Using whole-genome transcriptome and CGH analysis, we report that a
B. bronchiseptica
cystic fibrosis isolate, T44625, contains a distinct genomic content of virulence-associated genes and differentially expresses these genes compared to the sequenced model laboratory strain RB50, a rabbit isolate. The differential gene expression pattern correlated with unique phenotypes exhibited by T44625, which included lower motility, increased aggregation, hyperbiofilm formation, and an increased
in vitro
capacity to adhere to respiratory epithelial cells. Using a mouse intranasal infection model, we found that although defective in establishing high bacterial burdens early during the infection process, T44625 persisted efficiently in the mouse nose. By documenting the unique genomic and phenotypic attributes of T44625, this report provides a blueprint for understanding the successful zoonotic potential of
B. bronchiseptica
and other zoonotic bacteria.
An uncommon complication of acute stroke thrombolysis
Authors: Allan Morven , Zachariah George , Kordzadeh Ali , Umachandran Velaitham 
Published: January 2014

Thrombolysis with tissue plasminogen activator is a well-established treatment for acute ischaemic stroke. We report a case of an 87-year-old woman who developed an acute ischaemic limb, on the background of stroke thrombolysis, and underwent an embolectomy. A rare but serious complication, there are few reports of similar thromboembolic events, particularly in patients with known atrial fibrillation as presented in this case. Early recognition of this rare complication may prevent long-term, and at times fatal, complications.
Implementation of an App-based neuromuscular training programme to prevent ankle sprains: a process evaluation using the RE-AIM Framework
Authors: Vriend Ingrid , Coehoorn Iris , Verhagen Evert 
Published: January 2014

Background/aimThe contemporary electronic media is regarded as a practical tool in the dissemination of preventive measures and interventions. For this purpose an App (free of charge) was developed including an efficacious programme for the prevention of ankle sprain recurrences. This study evaluated the implementation effectiveness of this ‘Versterk je Enkel’ App.MethodsThe App was evaluated within its practical context using the Reach Effectiveness Adoption Implementation Maintenance (RE-AIM) Framework. The launch of the App was accompanied by a press release, website banners, as well as online and offline advertisements. Data for the evaluation of the App were objectively registered through Google analytics. Data were obtained in February 2013 based on 25 781 users resulting in follow-ups of 18 months (iOS version) and 15 months (Android version), respectively. Users questionnaires provided a qualitative view of the objectively assessed measures (n=82) to gain insight into the demographics of users, reasons to download, user experience and how the information was used.ResultsThe App reached only 2.6% of the projected target population. User ratings for the App's relevancy, clarity, usefulness, appeal, information and reliability were high. App usage indicates that compliance with the embedded programme was low.ConclusionsAlthough the App was well received by the users, targeted efforts are required to ensure proper uptake and usage of the App by the target population. This also holds true for eHealth and mHealth efforts aimed at athlete care and injury prevention in general.
Using quality improvement to optimise paediatric discharge efficiency
Authors: White Christine M , Statile Angela M , White Denise L , Elkeeb Dena , Tucker Karen , Herzog Diane , Warrick Stephen D , Warrick Denise M , Hausfeld Julie , Schondelmeyer Amanda , Schoettker Pamela J , Kiessling Pamela , Farrell Michael , Kotagal Uma , Ryckman Frederick C 
Published: January 2014

BackgroundBed capacity management is a critical issue facing hospital administrators, and inefficient discharges impact patient flow throughout the hospital. National recommendations include a focus on providing care that is timely and efficient, but a lack of standardised discharge criteria at our institution contributed to unpredictable discharge timing and lengthy delays. Our objective was to increase the percentage of Hospital Medicine patients discharged within 2 h of meeting criteria from 42% to 80%.MethodsA multidisciplinary team collaborated to develop medically appropriate discharge criteria for 11 common inpatient diagnoses. Discharge criteria were embedded into electronic medical record (EMR) order sets at admission and could be modified throughout a patient's stay. Nurses placed an EMR time-stamp to signal when patients met all discharge goals. Strategies to improve discharge timeliness emphasised completion of discharge tasks prior to meeting criteria. Interventions focused on buy-in from key team members, pharmacy process redesign, subspecialty consult timeliness and feedback to frontline staff. A P statistical process control chart assessed the impact of interventions over time. Length of stay (LOS) and readmission rates before and after implementation of process measures were compared using the Wilcoxon rank-sum test.ResultsThe percentage of patients discharged within 2 h significantly improved from 42% to 80% within 18 months. Patients studied had a decrease in median overall LOS (from 1.56 to 1.44 days; p=0.01), without an increase in readmission rates (4.60% to 4.21%; p=0.24). The 12-month rolling average census for the study units increased from 36.4 to 42.9, representing an 18% increase in occupancy.ConclusionsThrough standardising discharge goals and implementation of high-reliability interventions, we reduced LOS without increasing readmission rates.
Anticolorectal cancer activity of the omega-3 polyunsaturated fatty acid eicosapentaenoic acid
Authors: Cockbain Andrew J , Volpato Milene , Race Amanda D , Munarini Alessandra , Fazio Chiara , Belluzzi Andrea , Loadman Paul M , Toogood Giles J , Hull Mark A 
Published: January 2014

BackgroundOral administration of the omega-3 fatty acid eicosapentaenoic acid (EPA), as the free fatty acid (FFA), leads to EPA incorporation into, and reduced growth of, experimental colorectal cancer liver metastases (CRCLM).Design:We performed a Phase II double-blind, randomised, placebo-controlled trial of EPA-FFA 2 g daily in patients undergoing liver resection surgery for CRCLM. The patients took EPA-FFA (n=43) or placebo (n=45) prior to surgery. The primary end-point was the CRCLM Ki67 proliferation index (PI). Secondary end-points included safety and tolerability of EPA-FFA, tumour fatty acid content and CD31-positive vascularity. We also analysed overall survival (OS) and disease-free survival (DFS).ResultsThe median (range) duration of EPA-FFA treatment was 30 (12–65) days. Treatment groups were well matched with no significant difference in disease burden at surgery or preoperative chemotherapy. EPA-FFA treatment was well tolerated with no excess of postoperative complications. Tumour tissue from EPA-FFA-treated patients demonstrated a 40% increase in EPA content (p=0.0008), no difference in Ki67 PI, but reduced vascularity in ‘EPA-naïve’ individuals (p=0.075). EPA-FFA also demonstrated antiangiogenic activity in vitro. In the first 18 months after CRCLM resection, EPA-FFA-treated individuals obtained OS benefit compared with placebo, although early CRC recurrence rates were similar.ConclusionsEPA-FFA therapy is safe and well tolerated in patients with advanced CRC undergoing liver surgery. EPA-FFA may have antiangiogenic properties. Remarkably, limited preoperative treatment may provide postoperative OS benefit. Phase III clinical evaluation of prolonged EPA-FFA treatment in CRCLM patients is warranted.Trial Identifier:ClinicalTrials.govNCT01070355.
Low-complexity scalable distributed multicamera tracking of humans
Authors: Gruenwedel Sebastian , Jelaca Vedran , Nino-Castaneda Jorge Oswaldo , Hese Peter van , Cauwelaert Dimitri van , Haerenborgh Dirk van , Veelaert Peter , Philips Wilfried 
Published: January 2014

Real-time tracking of people has many applications in computer vision, especially in the domain of surveillance. Typically, a network of cameras is used to solve this task. However, real-time tracking remains challenging due to frequent occlusions and environmental changes. Besides, multicamera applications often require a trade-off between accuracy and communication load within a camera network. In this article, we present a real-time distributed multicamera tracking system for the analysis of people in a meeting room. One contribution of the article is that we provide a
scalable
solution using smart cameras. The system is scalable because it requires a very small communication bandwidth and only light-weight processing on a “fusion center” which produces final tracking results. The fusion center can thus be cheap and can be duplicated to increase reliability.

In the proposed decentralized system all low level video processing is performed on smart cameras. The smart cameras transmit a compact high-level description of moving people to the fusion center, which fuses this data using a Bayesian approach. A second contribution in our system is that the camera-based processing takes feedback from the fusion center about the most recent locations and motion states of tracked people into account. Based on this feedback and background subtraction results, the smart cameras generate a best hypothesis for each person.
We evaluate the performance (in terms of precision and accuracy) of the tracker in indoor and meeting scenarios where individuals are often occluded by other people and/or furniture. Experimental results are presented based on the tracking of up to 4 people in a meeting room of 9 m by 5 m using 6 cameras. In about two hours of data, our method has only 0.3 losses per minute and can typically measure the position with an accuracy of 21 cm. We compare our approach to state-of-the-art methods and show that our system performs at least as good as other methods. However, our system is capable to run in real-time and therefore produces instantaneous results.
Radio diversity for reliable communication in sensor networks
Authors: Kusy Branislav , Abbott David , Richter Christian , Huynh Cong , Afanasyev Mikhail , Hu Wen , Brünig Michael , Ostry Diethelm , Jurdak Raja 
Published: January 2014

Radio connectivity in wireless sensor networks is highly intermittent due to unpredictable and time-varying noise and interference patterns in the environment. Because link qualities are not predictable prior to deployment, current deterministic solutions to unreliable links, such as increasing network density or transmission power, require overprovisioning of network resources and do not always improve reliability.
We propose a new dual-radio network architecture to improve communication reliability in wireless sensor networks. Specifically, we show that radio transceivers operating at well-separated frequencies and spatially separated antennas offer robust communication, high link diversity, and better interference mitigation. We derive the optimal parameters for the dual-transceiver setup from frequency and space diversity in theory. We observe that frequency diversity holds the most benefits as long as the antennas are sufficiently separated to prevent coupling. Our experiments on an indoor/outdoor testbed confirm the theoretical predictions and show that radio diversity can significantly improve end-to-end delivery rates and network stability at only a small increase in energy cost over a single radio. Simulation experiments further validate the improvements in multiple topology configurations, but also reveal that the benefits of radio diversity are coupled to the number of available routing paths to the destination.
The Solvability and Optimal Controls for Some Fractional Impulsive Equations of Order1< α<2
Authors: Liu Xianghu , Liu Zhenhai , Bin Maojun 
Published: January 2014

We study the existence of solutions and optimal controls for some fractional impulsive equations of order1< α<2. By means of Gronwall’s inequality and Leray-Schauder’s fixed point theorem, the sufficient condition for the existence of solutions and optimal controls is presented. Finally, an example is given to illustrate our main results.
Morphological and Functional Adaptation of Left and Right Atria Induced by Training in Highly Trained Female Athletes
Authors: D’Ascenzi Flavio , Pelliccia Antonio , Natali Benedetta Maria , Zacà Valerio , Cameli Matteo , Alvino Federico , Malandrino Angela , Palmitesta Paola , Zorzi Alessandro , Corrado Domenico , Bonifazi Marco , Mondillo Sergio 
Published: January 2014

Background—
Exercise is able to induce atrial remodeling in top-level athletes. However, evidence is mainly limited to men and based on cross-sectional studies. The aim of this prospective, longitudinal study was to investigate whether exercise is able to influence left and right atrial morphology and function also in female athletes.

Methods and Results—

Two-dimensional echocardiography was performed before season and after 16 weeks of intensive training in 24 top-level female athletes. Left and right atrial myocardial deformation was assessed by two-dimensional speckle-tracking echocardiography. Left atrial volume index (24.0±3.6 versus 26.7±6.9 mL/m
2
;
P
<0.001) and right atrial volume index (15.66±3.09 versus 20.47±4.82 mL/m
2
;
P
<0.001) significantly increased after training in female athletes. Left atrial global peak atrial longitudinal strain and peak atrial contraction strain significantly decreased after training in female athletes (43.9±9.5% versus 39.8±6.5%;
P
<0.05 and 15.5±4.0% versus 13.9±4.0%;
P
<0.05, respectively). Right atrial peak atrial longitudinal strain and peak atrial contraction strain showed a similar, although non-significant decrease (42.8±10.6% versus 39.3±8.3%; 15.6±5.6% versus 13.1±6.1%, respectively). Neither biventricular
E
/
e
′ ratio nor biatrial stiffness changed after training, suggesting that biatrial remodeling occurs in a model of volume rather than pressure overload.

Conclusions—
Exercise is able to induce biatrial morphological and functional changes in female athletes. Biatrial enlargement, with normal filling pressures and low atrial stiffness, is a typical feature of the heart of female athletes. These findings should be interpreted as physiological adaptations to exercise and should be considered in the differential diagnosis with cardiomyopathies.
Revision of the checkered beetle genus Erymanthus Spinola, 1841 (Coleoptera, Cleridae, Clerinae)
Authors: GERSTMEIER ROLAND , SALVAMOSER MAX 
Published: January 2014

The genus Erymanthus Spinola, 1841 is taxonomically revised to include sixteen species. The species E. transversopustulatus n. sp. is described as new. Erymanthus concoloripennis Pic, 1932, n. syn. is placed in synonymy with E. bicolor Chapin, 1924, E. horridus var. semirufus Kuwert, 1893 is recognized as a distinct and valid species E. semirufus; E. variegatus Pic, 1950 n. syn. is synonymized with E. pustulosus Quedenfeldt, 1888. A key to the species, color photographs, genital figures and distribution maps are provided. Erymanthus ampliatus Hintz, 1902 is transferred to the genus Gyponyx Gorham, 1883 (= Gyponyx amplicatus (Hintz, 1902) n. comb.).
Opportunities to Intercalibrate Radiometric Sensors from International Space Station
Authors: Roithmayr C. M. , Lukashin C. , Speth P. W. , Young D. F. , Wielicki B. A. , Thome K. J. , Kopp G. 
Published: January 2014

Abstract
Highly accurate measurements of Earth’s thermal infrared and reflected solar radiation are required for detecting and predicting long-term climate change. Consideration is given to the concept of using the International Space Station to test instruments and techniques that would eventually be used on a dedicated mission, such as the Climate Absolute Radiance and Refractivity Observatory (CLARREO). In particular, a quantitative investigation is performed to determine whether it is possible to use measurements obtained with a highly accurate (0.3%, with 95% confidence) reflected solar radiation spectrometer to calibrate similar, less accurate instruments in other low Earth orbits. Estimates of numbers of samples useful for intercalibration are made with the aid of yearlong simulations of orbital motion. Results of this study support the conclusion that the International Space Station orbit is ideally suited for the purpose of intercalibration between spaceborne sensors.
Model Tuning with Canonical Correlation Analysis
Authors: Marzban Caren , Sandgathe Scott , Doyle James D. 
Published: January 2014

Abstract
Knowledge of the relationship between model parameters and forecast quantities is useful because it can aid in setting the values of the former for the purpose of having a desired effect on the latter. Here it is proposed that a well-established multivariate statistical method known as canonical correlation analysis can be formulated to gauge the strength of that relationship. The method is applied to several model parameters in the Coupled Ocean–Atmosphere Mesoscale Prediction System (COAMPS) for the purpose of “controlling” three forecast quantities: 1) convective precipitation, 2) stable precipitation, and 3) snow. It is shown that the model parameters employed here can be set to affect the sum, and the difference between convective and stable precipitation, while keeping snow mostly constant; a different combination of model parameters is shown to mostly affect the difference between stable precipitation and snow, with minimal effect on convective precipitation. In short, the proposed method cannot only capture the complex relationship between model parameters and forecast quantities, it can also be utilized to optimally control certain combinations of the latter.
Social and behavioural risk factors for HIV infection among the wives of labour migrants in Nepal
Authors: Thapa Subash , Bista Nirmala , Timilsina Suraj , Buntinx Frank , Mathei Catharina 
Published: January 2014

Summary Labour migration has increased the risk of HIV infection among the wives of labour migrants in Nepal. We conducted a matched case-control study to identify the social and behavioural factors for HIV infection among the wives of labour migrants in Nepal. We interviewed 112 wives of labour migrants diagnosed with HIV (cases) and 112 wives of labour migrants testing negative for HIV (controls) and used logistic regression analysis to assess independent factors associated with HIV infection. Literacy status was the only one woman-related social factor associated with HIV infection. Meanwhile literacy status, age when going abroad for the first time and country of migration were the husband-related social factors and alcohol consumption, living alone abroad and having an unpaid partner abroad were the husband-related behavioural factors associated with HIV infection in the wives. Given the husband-related social and behavioural factors are mostly determining the risk of HIV infection in the wives in our study, prevention efforts must incorporate behaviour change approaches targeting specifically to labour migrants and also to their wives.
Symptomatic fructose malabsorption in irritable bowel syndrome: A prospective study
Authors: Melchior Chloé , Gourcerol Guillaume , Déchelotte Pierre , Leroi Anne-Marie , Ducrotté Philippe 
Published: January 2014

IntroductionFructose can trigger or worsen symptoms in irritable bowel syndrome (IBS) patients. The aim of this study was to determine the prevalence of symptomatic fructose malabsorption in IBS patients and to test whether the patient's characteristics can help to detect a fructose malabsorption.Materials and methodsNinety Rome III IBS patients (predominant diarrhoea (IBS‐D): 31%, predominant constipation (IBS‐C): 18%, mixed type (IBS‐M): 51%) were included prospectively. After exclusion of a small intestinal bacterial overgrowth by a glucose breath test, fructose malabsorption was assessed by a five‐hour breath test, with symptom monitoring, after a 25 g load of fructose. An increase of more than 20 ppm of hydrogen (H2) or methane (CH4) levels in the exhaled air led to the diagnosis of malabsorption.ResultsFructose test was abnormal in 20/90 patients among whom only 35% were intolerant, with a simultaneous rise of H2 /CH4 levels and the onset of abdominal discomfort or diarrhoea. IBS characteristics were not predictive even if young (p  = 0.031) and male IBS patients (p  = 0.029) were at higher risk of malabsorption. At variance, 18 additional patients experienced intestinal symptoms during the test despite normal fructose absorption.DiscussionAfter a 25 g fructose load, symptomatic fructose malabsorption and intolerance without malabsorption were detected in 22% and 28% of IBS patients respectively.
Production of a reference transcriptome and transcriptomic database (EdwardsiellaBase) for the lined sea anemone, Edwardsiella lineata, a parasitic cnidarian
Authors: Stefanik Derek J , Lubinski Tristan J , Granger Brian R , Byrd Allyson L , Reitzel Adam M , DeFilippo Lukas , Lorenc Allison , Finnerty John R 
Published: January 2014

Abstract

Background
The lined sea anemone Edwardsiella lineata is an informative model system for evolutionary-developmental studies of parasitism. In this species, it is possible to compare alternate developmental pathways leading from a larva to either a free-living polyp or a vermiform parasite that inhabits the mesoglea of a ctenophore host. Additionally, E. lineata is confamilial with the model cnidarian Nematostella vectensis, providing an opportunity for comparative genomic, molecular and organismal studies.

Description
We generated a reference transcriptome for E. lineata via high-throughput sequencing of RNA isolated from five developmental stages (parasite; parasite-to-larva transition; larva; larva-to-adult transition; adult). The transcriptome comprises 90,440 contigs assembled from >15 billion nucleotides of DNA sequence. Using a molecular clock approach, we estimated the divergence between E. lineata and N. vectensis at 215–364 million years ago. Based on gene ontology and metabolic pathway analyses and gene family surveys (bHLH-PAS, deiodinases, Fox genes, LIM homeodomains, minicollagens, nuclear receptors, Sox genes, and Wnts), the transcriptome of E. lineata is comparable in depth and completeness to N. vectensis. Analyses of protein motifs and revealed extensive conservation between the proteins of these two edwardsiid anemones, although we show the NF-κB protein of E. lineata reflects the ancestral structure, while the NF-κB protein of N. vectensis has undergone a split that separates the DNA-binding domain from the inhibitory domain. All contigs have been deposited in a public database (EdwardsiellaBase), where they may be searched according to contig ID, gene ontology, protein family motif (Pfam), enzyme commission number, and BLAST. The alignment of the raw reads to the contigs can also be visualized via JBrowse.

Conclusions
The transcriptomic data and database described here provide a platform for studying the evolutionary developmental genomics of a derived parasitic life cycle. In addition, these data from E. lineata will aid in the interpretation of evolutionary novelties in gene sequence or structure that have been reported for the model cnidarian N. vectensis (e.g., the split NF-κB locus). Finally, we include custom computational tools to facilitate the annotation of a transcriptome based on high-throughput sequencing data obtained from a “non-model system.”