bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistochemical Studies of Infiltrating Cells in Early and Chronic Lesions of Psoriasis
Authors: Onuma Sumi 
Published: April 2015

AbstractThe expression of surface antigens on infiltrating cells, epidermal keratinocytes, and dendritic cells in biopsy specimens from 31 patients with psoriasis was examined immunohistochemically. The specimens were divided into early‐phase and chronic‐phase groups and then examined in a double blind manner.Among the infiltrating cells in the epidermis, CD4‐positive cells were dominant in the early phase; CD8‐positive cells were dominant in the chronic phase, resulting in a markedly decreased CD4/CD8 ratio in the latter. On the other hand, among the infiltrating cells in the dermal papillae, CD4‐positive cells were dominant in both the early and chronic phases; both CD4‐positive and CD8‐positive cells were more dominant in the chronic phase than in the early one. However, the CD4/CD8 ratios were decreased in both the dermal papillae and the epidermis in the chronic phase. CD1‐positive dendritic cells (probably Langerhans cells) were more numerous in the chronic phase than in the early phase. There were no significant differences between the early and chronic phases with regard to the expression of HLA‐DR and HLA‐DQ antigens on the infiltrating cells. However, the HLA‐DR antigens and ICAM‐1 (intercellular adhesion molecule‐1) were more strongly expressed on epidermal keratinocytes in the chronic phase than in the early phase. LFA‐1α (lymphocyte function‐associated antigen‐1α)‐positive cells were also significantly more numerous in the chronic phase than in the early one, consistent with the expression of HLA‐DR antigens and ICAM‐1 on keratinocytes mentioned above. On the other hand, VLA‐4 (integrin α4β1) positive cells were expressed more abundantly in the epidermis in the early phase than in the chronic phase.These results suggest, first, that the chronic phase of psoriasis is as immunologically active as or more active than the early phase. Second, CD4‐positive T cells are more important than CD8‐positive T cells in the early phase of psoriasis; CD8‐positive rather than CD4‐positive T cells are more important in the chronic phase. Third, the LFA‐1/ICAM‐1 pathway may play an important role with regard to cell adhesion of the infiltrating cells in the psoriatic lesions in disease exacerbation or prolongation, whereas the VLA‐4/VCAM‐1 (vascular cell adhesion molecule‐1) pathway may be more important in disease onset.
Ultraviolet Irradiation Downregulates the IL‐1 Receptor in Pam 212 Keratinocytes
Authors: Nozaki Shigeyuki , Sauder Daniel N. 
Published: April 2015

AbstractThere is increasing evidence that keratinocytes, by virture of production of cytokines, may participate in inflammatory and immune responses in the skin. The expression of keratinocyte cytokines can be modulated by various exogenous and endogenous agents, including ultraviolet light (UV) and cytokines themselves. We have recently shown that augmentation of GM‐CSF expression by UVB irradiation is mediated by UV‐induced IL‐1 in Pam 212 keratinocytes. This may suggest that an autocrine mechanism exists in this murine keratinocyte cell line. In order to further clarify the mechanism by which UV irradiation augments GM‐CSF, this study was undertaken to assess the effect of UV on the expression of IL‐1 receptor (IL‐1R) in Pam 212 keratinocytes. UVB irradiation (35 or 70 J/m2) significantly downregulates the expression of IL‐1R mRNA. IL‐1R mRNA remains downregulated for 12h after UV, then slowly returns to the steady state level by 24h to 32h after UV. On the other hand, UV augments IL‐1 mRNA in a biphasic pattern with an initial phase (by 6h after UV) and a late phase (24h and 48h after UV). The results of these studies indicate that modulation of IL‐1R and IL‐1 itself by UVB irradiation are important in mediating autocrine cytokine networks in keratinocytes.
Molecular Genetic Analysis of the von Recklinghausen Neurofibromatosis (NF1) Gene Using Polymerase Chain Reaction‐Single Strand Conformation Polymorphism (PCR‐SSCP) Method
Authors: Sawada Shun'ichi , Honda Mariko , Niimura Michihito 
Published: April 2015

AbstractVon Recklinghausen neurofibromatosis (NF1) is a common autosomal dominant disorder characterized by abnormalities in multiple tissues derived from the embryonic neural crest. The NF1 gene has been mapped to the pericentromeric region of the long arm of chromosome 17. Chromosome walking and sequencing of the NF1 gene have extended it's open reading frame; to date 49 exons have been identified. To investigate the mutation of the NF1 gene, the polymerase chain reaction‐single strand conformation polymorphism (PCR‐SSCP) method was applied to 4 exons of NF1 genes. We examined the DNAs from 49 samples, including those of 23 Japanese patients with NF1 (4 of these patients developed malignant schwannoma), a patient with segmental neurofibromatosis, and 14 clinically normal controls. A mutational band was detected in an exon of a tumor DNA extracted from a malignant schwannoma of a female NF1 patient. However, the mutation was not found in the germ line DNA of this patient. No mutations were detected in the other samples.
Tinea Corporis Due to Trichophyton verrucosum: Report of a Patient from the San'in District
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractA 70‐year‐old Japanese housewife, a resident of Shimane Prefecture, developed an erythematous, annular skin lesion on her right forearm. Mycological examinations revealed that it was tinea corporis caused by Trichophyton verrucosum (T. verrucosum). This fungus was then shown to have been transmitted to her from young dairy cattle introduced from the Hokkaido District to the ranch of her son. To the best of our knowledge, she is the first reported patient with tinea due to T. verrucosum who was infected in the San'in District.
Stoichiometry of the Reaction Catalyzed by Skin Sulfhydryl Oxidase
Authors: Yamada Hiromichi , Suga Yasushi , Takamori Kenji , Ogawa Hideoki 
Published: April 2015

AbstractThe stoichiometry of the reaction catalyzed by skin sulfhydryl oxidase was investigated. Dithiothreitol (DTT) was used as the substrate for skin sulfhydryl oxidase. The consumption of DTT, consumption of oxygen, and production of hydrogen peroxide were measured during the enzyme reaction. The molar ratio of DTT:O2:H2O2 in the enzyme reaction was 1:1.02:0.89. Correspondingly, the stoichiometry of the enzyme reaction was calculated to be R(SH)2 + O2 → + H2O2.
Interleukin 8 in Behçet's Disease
Authors: Itoh Rie , Takenaka Teruyoshi , Okitsu‐Negishi Shoko , Matsushima Kouji , Mizoguchi Masako 
Published: April 2015

AbstractActivated peripheral polymorphonuclear leukocytes (PMNs) and infiltration of PMNs into the lesions are characteristic findings of Behçet's disease (BD). A variety of cytokines, including interleukin 8 (IL‐8), have been shown to activate PMNs. To investigate the role of IL‐8 in the development of BD lesions, IL‐8 production in vivo and in vitro was examined in 25 BD patients. IL‐8 levels measured by ELISA in the non stimulated culture supernatants of peripheral mononuclear cells (MNCs) were higher in patients with active BD than in those with inactive BD or normal controls. Without LPS stimulation, IL‐8 mRNA expression in incubated MNCs detected by Northern blot analysis was higher in active BD patients than in controls. Polarization assay confirmed the accelerated activity of PMN isolated from patients with active BD. However, these PMNs did not respond to IL‐8 as strongly as to FMLP (an exogenous stimulator); a possible reason is that the PMNs of these patients are constantly exposed to IL‐8 in vivo. Immunohistochemically, MNCs, endothelial cells and fibroblasts in BD lesions were positively stained by anti‐IL‐8 antibody.These data indicate that the production of IL‐8 may be accelerated in inactive BD and that IL‐8 may play an important role in the pathogenesis of BD.
Study of the Efficacy of Antiseptic Handrub Lotions with Hand Washing Machines
Authors: Namura Shoko , Nishijima Setsuko , Mitsuya Kaoru , Asada Yasuo 
Published: April 2015

AbstractThe effectiveness of quick handwashing in regards to four alcohol‐based handrub lotions, including two products already on the market and our original lotions 1, 2, with two different kinds of hand washing machines was studiedin vivo. We also tried to evaluate the efficacy of the four lotionsin vitro.Thein vivotesting of each lotion, including ethanol, was evaluated using two hand washing methods, first with and then without a previous soap wash. Computerized image analysis was used to calculate the bacterial count on the hand surface.The results showed that a 3 second application of the original lotion 2 (0.5% chlorhexidine in a 77% ethyl alcohol solution) preceded by a soap wash eradicated 89.3% of the bacteria on the hands; this was the highest reduction rate of all four detergents. AROKULIN‐E (67.9% ethyl alcohol solution) without a prior handwashing produced the lowest reduction, 49.1% against hand surface bacteria. These results were compatible with thosein vitro. It seems that an alcohol‐based solution containing an effective antimicrobial detergent preceded by a soap wash is neccessary to acceptably reduce hand surface bacteria.
Cowden's Disease: Report of the First Case in a Chinese
Authors: Yang Jen‐Hung , Cheng Huey‐Mann , Wang Long‐Rule , Chu Kan‐C 
Published: April 2015

AbstractA 19‐year‐old Chinese female presenting mucocutaneous manifestations including facial trichilemmomas, oral papillomatosis, and acral keratosis was diagnosed, according to the criteria proposed by Salem and Steck, as a definite case of Cowden's disease, although no members of her family were affected. The cutaneous reticulohistiocytic granuloma and congenital nystagmus of eyes that occurred in this case were new combinations in the syndrome. To the best of our knowledge, this is the first documented case of Cowden's disease in a Chinese.
Cutaneous Mesenchymal Hamartoma
Authors: Chang Sung Nam , Lee Seung Hun , Ahn Sung Ku 
Published: April 2015

AbstractA case of a cutaneous mesenchymal hamartoma occurring on the thigh of a newborn infant is reported. Microscopically, the solitary tumorous lesion consisted of an intimate mixture of fibrocollagenous bundles, islands of mature fat cells, distorted blood vessels, and eccrine sweat glands. Many mast cells were scattered throughout the fibrocollagenous bundles. Ultrastructurally, most tumor cells were suggestive of undifferentiated mesenchymal cells.
Effect of Peplomycin or Camptothecin‐11 on X‐ray Skin Injuries of ICR Hairless Mice
Authors: Kawahara Kenichi , Kano Eiichi , Ueda Keiichi 
Published: April 2015

AbstractIn interdisciplinary oncotherapy by radiation and chemical substances, the injurious effects of radiation on the normal skin are occasionally modified by combined chemical substances. In the present experiment, the possible modifying effects of Peplomycin (PEP, 30 mg/kg body weight) and Camptothecin‐11, an inhibitor of DNA topoisomerase I (CPT‐11, 15 and 50 mg/kg body weight), on radiation skin injury were studied. Macroscopic changes on the thigh skin of male ICR hairless mice (at 8 weeks of age) induced by 10 Gy X‐ray irradiation alone and the combined treatment with the anticancer substances were estimated by a modified scoring first reported by Lowy and Baker. Intraperitoneal administration of PEP significantly enhanced radiation skin injury; while CPT‐11 (15 mg/kg), within the clinical dose range and in the similar molarities to that of PEP, did not show any appreciable modification effect. Treatment with CPT‐11 (50 mg/kg) showed a radiation enhancement without significant difference. The chemotherapeutic radioenhancement ratios of the combined treatments of X‐ray and PEP or CPT‐11 (15 or 50 mg/kg) were 1.49, 1.17 or 1.34, respectively. Treatment with PEP at the accepted dose showed a radiation enhancement, while CPT‐11 did not show any radiation enhancement within the clinical dose.
Primary Actinomycosis of the Breast: A Clinical Review and a Case Report
Authors: Jain Bhupendra Kumar , Sehgal Virendra Nath , Jagdish S. , Ratnakar Chiganti , Smile S. Robinson 
Published: April 2015

AbstractAn uncommon situation of primary actinomycosis of the breast is reported in a 40‐year‐old woman who presented with a lump, clinically simulating malignancy, in the right breast. Diagnosis of actinomycosis was established by incision biopsy. Resolution of infection, while conserving the breast, was achieved by timely diagnosis, limited surgery, and effective antibiotic therapy. Diagnostic failures leading to avoidable mastectomies have not been uncommon in cases previously reported in the literature. It is, therefore, imperative that this condition be considered in the differential diagnosis of intractable breast abscess and malignancy.
Lupus Erythematosus‐like Syndrome Induced by Thiamazole and Propylthiouracil
Authors: Sato‐Matsumura Kazuko C. , Koizumi Hiroko , Matsumura Tetsuri , Takahashi Tatsuro , Adachi Koichi , Ohkawara Akira 
Published: April 2015

AbstractA 17‐year‐old Japanese woman developed a lupus erythematosus‐like syndrome during treatment for Graves' disease with thiamazole and propylthiouracil. Erythema, arthralgia, and low grade fever developed during therapy with thiamazole; purplish‐red erythema developed during therapy with propylthiouracil. Antinuclear antibodies, anti‐single‐stranded DNA antibodies, and anti‐double‐stranded DNA antibodies were positive throughout the administration of these two drugs. Eruptions and other symptoms improved after their discontinuation. The titers of autoantibodies also decreased two months after withdrawal. The patient had HLA DR4.
Granulocyte Colony‐Stimulating Factor (G‐CSF) and Granulocyte Macrophage Colony‐Stimulating Factor (GM‐CSF) in Behçet's Disease
Authors: Takahama Hideto , Itoh Rie , Inoue‐Komatsu Chiyuki , Furusawa Shuichi , Takahashi Hisashi , Mizoguchi Masako 
Published: April 2015

AbstractIncreases in the number and activity of peripheral polymorphonuclear neutrophils (PMNs) is often found in Behçet's disease (BD), indicating that PMN may play an important role in the pathogenesis of this disorder. It has recently been reported that G‐CSF and GM‐CSF, a family of hematopoietic growth factors, enhance PMN activity. To explore the role of these two CSFs in BD, we first examined the chemotactic response of PMNs to these CSFs by performing a polarization assay. PMN response to G‐CSF in BD patients was lower than that in controls, while PMN response to GM‐CSF was similar in patients and controls. However, PMNs from BD patients showed an enhanced chemotactic response to N‐formyl‐L‐methionyl‐leucyl‐phenylalanine. Thus, it is speculated that the PMNs of the patients might have already been activated in vivo by G‐CSF and thus could not respond further to this agent in vitro. We examined G‐CSF and GM‐CSF mRNA expressions in peripheral mononuclear cells stimulated with LPS, PMA, and Con A by Northern hybridization. G‐CSF mRNA expression levels in BD patients were higher than in the controls, while GM‐CSF mRNA expression levels were lower than in the controls. We also examined the serum levels of the two CSFs by ELISA and EIA. However, all levels of the two CSFs in both patients and controls were not detectable, except in the case of one BD patient in the active stage of the disease, who showed high levels of G‐CSF, but not of GM‐CSF. Based on the present findings, it is suggested that G‐CSF may be more intensively involved in BD than GM‐CSF.
An Immunohistochemical Study of Sebaceous Carcinoma with Anti‐Keratin Monoclonal Antibodies: Comparison with Other Skin Cancers
Authors: Ansai Shin‐ichi , Katagata Yohtaro , Yoshikawa Ken‐ichi , Hashimoto Hideki , Hozumi Yutaka , Kondo Shigeo , Aso Kazuo 
Published: April 2015

AbstractFormalin‐fixed and paraffin‐embedded tissue specimens of six cases of extraocular sebaceous carcinoma were studied immunohistochemically with eight anti‐keratin monoclonal antibodies, 34βB4, 35βH11, Ks13.1, Ks19.1, PKK1, LP34, KL1 and AE1. The staining patterns of sebaceous carcinoma were compared with those of normal sebaceous glands and other skin cancers which should be distinguished from sebaceous carcinoma histopathologically. The other skin cancers compared were eccrine porocarcinoma, malignant clear cell hidradenoma, extramammary Paget's disease with underlying adenocarcinoma, malignant trichilemmoma, and squamous cell carcinoma. Most cases of sebaceous carcinoma were stained with 35βH11, Ks19.1, LP34, KL1 and AE1, while normal sebaceous glands were positive only with 35βH11, LP34, KL1 and AE1. By immunostaining, sebaceous carcinoma was distinguishable from extramammary Paget's disease with underlying adenocarcinoma, squamous cell carcinoma, malignant trichilemmoma, and eccrine porocarcinoma, but was not clearly distinguishable from malignant clear cell hidradenoma. These findings demonstrate that sebaceous carcinoma shows positive reactions with antibodies to simple epithelial keratin, probably as a result of neoplastic transformation, and that immunohistochemical examination using anti‐keratin monoclonal antibodies is useful in distinguishing sebaceous carcinoma from several other skin cancers.
Scanning and Transmission Electron Microscopic Studies of Lesional Epidermis in Herpes Zoster
Authors: Tanaka Katsumi , Tsuda Shingo , Sasai Yoichiro 
Published: April 2015

AbstractThe epidermal skin lesions of herpes zoster were studied by scanning (SEM) and transmission electron microscopy (TEM). When erythematous lesions were observed by TEM, many of the infected keratinocytes showed evidence of cell degeneration, being characterized by swollen nuclei, disappearance of desmosomes, and widening of intercellular spaces. Macrophages and/or lymphocytes migrated through the intercellular spaces between degenerated keratinocytes. In the vesicular lesions, SEM and TEM showed some infiltrating neutrophils, directly adhering to the virus‐infected keratinocytes, with swollen nuclei and irregularly clumped chromatin. In some specimens, balloon‐degenerated keratinocytes were observed in the cavity. In the pustular stage, ruptured keratinocytes and numerous neutrophils were observed in the reticular‐degenerated epidermal tissue. These results suggest that, in herpes zoster, the epidermal damage may be due, at least in part, to cell‐mediated host immunity as well as to the cytopathic effect of varicella‐zoster virus.
Multiple Trichoepitheliomas with Rare Features
Authors: D'Souza Mariette , Garg Bhagat Ram , Ratnakar Chiganti , Agrawal Karoon 
Published: April 2015

AbstractA case of multiple trichoepitheliomas associated with epidermal cysts is reported. The trichoepithelioma lesions were florid and extensive with large nodulo‐cystic lesions on the face and a new variant simulating hidradenitis suppurativa near the gluteal cleft.
Coincidence of Vitiligo and Paget's Bone Disease in a Patient
Authors: Abraham Zeev , Glück Zahava , Kinarty Amalia , Lahat Nitza 
Published: April 2015

AbstractPaget's bone disease developed in a patient with vitiligo. Scrupulous physical examination excluded further systemic or cutaneous involvement. The immunological workup revealed a reversed CD4/CD8 ratio due to a very low CD4 cell percentage and almost negligible responses to PHA as well as Con A, T cell mitogens. The pathogenic significance of these results, which point to phenotypic and functional T cell defects, is discussed.
A Case of Herpetiform Pemphigus Associated with Lung Cancer
Authors: Kubota Yasuo , Yoshino Yutaka , Mizoguchi Masako 
Published: April 2015

AbstractWe describe a case of herpetiform pemphigus associated with lung cancer. Only one other documented association between herpetiform pemphigus and malignancy could be found in the Japanese dermatologic literature. However, it is very difficult to determine whether such an association is coincidence or a true relationship.
Erythema Gyratum Repens Associated with Hypereosinophilic Syndrome
Authors: Morita Akimichi , Sakakibara Noriyuki , Tsuji Takuo 
Published: April 2015

AbstractWe report a case of typical erythema gyratum repens lesions observed as a manifestation of idiopathic hypereosinophilic syndrome in a 63‐year‐old man. While erythema gyratum repens is usually associated with malignancy, an intensive search over a 30‐month period failed to reveal any evidence of neoplasm. With administration of dapsone, the typical gyrate lesions disappeared as the subject's hypereosinophilia improved.
A Contribution to the Understanding of the Heredity of Skin Tumors
Authors: Schnyder Urs W. 
Published: April 2015

AbstractAt least 21 multiple tumors of the skin are inherited autosomal‐dominantly. Eighteen are benign and three are malignant. One‐third of these tumors have no associated lesions, another third is associated with benign visceral lesions, and a final third belongs to the group of cancer associated genodermatoses. The solitary variants of these tumors, however, are sporadic, not inherited.
Basal Cell Carcinoma: Seven Years' Experience at the Institute of Dermatology in Bangkok
Authors: Nakjang Yaovares , Kullavanijaya Preya 
Published: April 2015

AbstractSixty‐seven patients with basal cell carcinoma (BCC) were observed at the Institute of Dermatology, Bangkok, Thailand between 1982 and 1989. The male:female ratio was 1:2. The average age of the patients at first examination was 60.6 years. Lesions in sun‐exposed areas were three times more frequent than lesions in covered areas. The principal location was the face, including the cheek, nose, periorbital area, ears, and forehead. The remainder were on the scalp, breast, abdomen, back, thigh, and knee. Ulceration was the most frequent symptom, followed by a mass. Histopathologically, pigmented BCC was the most frequent, followed by solid BCC, superficial BCC, adenoid BCC, morphoeic BCC, cystic BCC, basosquamous BCC, mixed carcinoma, keratotic BCC, and fibroepitheliomatous BCC. In patients younger than 50 years of age, solid or pigmented tumors were frequent. The morphoeic type was present only on the face and scalp. In females, solid BCC or adenoid BCC predominated. Only 4 of our 67 patients had histories of ingestion of herbal medicine with arsenic as one of the ingredients. Sixty‐one patients were treated surgically. There was only one case of tumor recurrence after surgery. The remaining patients were treated by electric cautery, liquid nitrogen, or irradiation. No patients developed distant metastasis.
A Case of Double Cancer: A Squamous Cell Carcinoma Arising from a Long Pierced Site on the Left Earlobe and a Solid Tubular Carcinoma of the Left Breast
Authors: Hagiwara Keisuke , Shinzato Rimi , Higa Tadashi , Toyama Kensei , Miyazato Hajime , Nonaka Shigeo 
Published: April 2015

AbstractA 43‐year‐old woman presented with a well differentiated squamous cell carcinoma of the left earlobe where a small hole for pierced type earrings had been made about 20 years earlier. A year after the removal of the tumor, a metastasis of a moderately differentiated squamous cell carcinoma to the left cervical lymph nodes was detected. A month later, a solid tubular carcinoma of the left breast was found. A modified radical neck dissection and a quadrantectomy with an axillary lymph node dissection were performed. A total of 5 courses of peplomycine‐mitomycine combinated therapy with 26 Gy of radiotherapy was administered for the cervical lesion. A month of postoperative chemotherapy with tegafur‐uracil and medroxyprogesterone acetate and a total of 56 Gy of radiotherapy to the mammary lesion were added. She has been well without recurrence for about a year after the operations. This case is not only a rare double cancer, a combination of a squamous cell carcinoma and a solid tubular carcinoma, but may also be the first case of a squamous cell carcinoma arising at a long pierced site on an earlobe for pierced type earrings.