bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti‐D alloimmunisation in pregnant women with DEL phenotype in China
Authors: Wang M. , Wang B. L. , Xu W. , Fan D. D. , Peng M. L. , Pan J. , Yao P. , Jiang G. M. , Wan X. J. 
Published: May 2015

SUMMARYObjectivesTo analyse anti‐D alloimmunisation in pregnant women with D‐elute (DEL) phenotype in China, for developing a predictive model to evaluate whether a person with the DEL phenotype can receive RhD‐positive blood.BackgroundAlloanti‐D acquired by pregnancy or transfusion is one of the major causes of both haemolytic disease among newborns and haemolytic transfusion reactions. To date, there is little data available about the antigenic properties and immunogenicity of extremely weak D variants known as DEL.MethodsRHD genotyping and D epitope mapping were performed using gene sequencing and comprehensive immunohaematological methods, respectively. DEL pregnant women carrying an RhD‐positive fetus were tested for the presence of alloanti‐D.ResultsA total of 130 of 142 (91·5%) pregnant women with a DEL phenotype were confirmed to carry the RHD (K409K) allele. Among 12 DEL women who appeared to have RHD‐CE‐D hybrid alleles, there were 1 RHD‐CE (4–7)‐D, 7 RHD‐CE(4–9)‐D, and 4 RHD‐CE (2–5)‐D alleles. Alloanti‐D antibodies were detected in 6 of 142 DEL women, and all the six women had the partial DEL phenotype.ConclusionThe data indicate that partial DEL women appear at risk of alloimmunization to the D antigen. RhD immune globulin prophylaxis is necessary for partial DEL women. Partial DEL patients should receive only RhD‐negative RBCs, whereas DEL patients with complete expression of antigen can safely receive RhD‐positive RBCs.
Bone density in apheresis donors and whole blood donors
Authors: Boot C. L. , Luken J. S. , van den Burg P. J. M. , de Kort W. L. A. M. , Koopman M. M. W. , Vrielink H. , van Schoor N. M. , den Heijer M. , Lips P. 
Published: May 2015

Apheresis donation using citrate causes acute decrease in serum calcium and increase in serum parathyroid hormone. Long‐term consequences, such as decrease in bone mineral density (BMD), are not known. In this study, we compared the BMD of 20 postmenopausal apheresis donors (mean donation number 115 times in up to 15 years) with that of 20 whole blood donors (for 15 years or more) aged 55–70. BMD in the lumbar spine was not lower in apheresis donors than in blood donors (mean ± SD 1·00 ± 0·18 vs. 0·92 ± 0·12, P = 0·09). In the hip, BMD was not different between the groups.
Predicted Distribution of Visceral Leishmaniasis Vectors (Diptera: Psychodidae; Phlebotominae) in Iran: A Niche Model Study
Authors: Hanafi‐Bojd A. A. , Rassi Y. , Yaghoobi‐Ershadi M. R. , Haghdoost A. A. , Akhavan A. A. , Charrahy Z. , Karimi A. 
Published: May 2015

SummaryVisceral leishmaniasis (VL) is an important vector‐borne disease in Iran. Till now, Leishmania infantum has been detected from five species of sand flies in the country including Phlebotomus kandelakii, Phlebotomus major s.l., Phlebotomus perfiliewi, Phlebotomus alexandri and Phlebotomus tobbi. Also, Phlebotomus keshishiani was found to be infected with Leishmania parasites. This study aimed at predicting the probable niches and distribution of vectors of visceral leishmaniasis in Iran. Data on spatial distribution studies of sand flies were obtained from Iranian database on sand flies. Sample points were included in data from faunistic studies on sand flies conducted during 1995–2013. MaxEnt software was used to predict the appropriate ecological niches for given species, using climatic and topographical data. Distribution maps were prepared and classified in ArcGIS to find main ecological niches of the vectors and hot spots for VL transmission in Iran. Phlebotomus kandelakii, Ph. major s.l. and Ph. alexandri seem to have played a more important role in VL transmission in Iran, so this study focuses on them. Representations of MaxEnt model for probability of distribution of the studied sand flies showed high contribution of climatological and topographical variables to predict the potential distribution of three vector species. Isothermality was found to be an environmental variable with the highest gain when used in isolation for Ph. kandelakii and Ph. major s.l., while for Ph. alexandri, the most effective variable was precipitation of the coldest quarter. The results of this study present the first prediction on distribution of sand fly vectors of VL in Iran. The predicted distributions were matched with the disease‐endemic areas in the country, while it was found that there were some unaffected areas with the potential transmission. More comprehensive studies are recommended on the ecology and vector competence of VL vectors in the country.
Genome Sequence of an Alphabaculovirus Isolated from the Oak Looper,
Lambdina fiscellaria,
Contains a Putative 2-Kilobase-Pair Transposable Element Encoding a Transposase and a FLYWCH Domain-Containing Protein
Authors: Rohrmann George F. , Erlandson Martin A. , Theilmann David A. 
Published: May 2015

ABSTRACT

The genome sequence of an alphabaculovirus isolated from
Lambdina fiscellaria
indicated that it is a novel member of a group II lineage. A putative transposable element was identified that contained two genes, including a transposase ortholog. These genes were most closely related to genes of the pea aphid
, Acyrthosiphon pisum.
Draft Genome Sequences of Three Capnocytophaga canimorsus Strains Isolated from Septic Patients
Authors: Manfredi Pablo , Renzi Francesco , Cornelis Guy R. 
Published: May 2015

ABSTRACT

Capnocytophaga canimorsus
is a bacterium from the normal oral flora of dogs and cats that causes rare generalized infections in humans. In an attempt to determine whether infections could be caused by a subset of strains and to identify pathogenicity factors, we sequenced the genomes of three strains isolated from human infections.
Draft Genome Sequences of Three Capnocytophaga cynodegmi Strains Isolated from the Oral Cavity of Healthy Dogs
Authors: Manfredi Pablo , Renzi Francesco , Cornelis Guy R. 
Published: May 2015

ABSTRACT

Here, we present the draft genome sequences of three strains of
Capnocytophaga cynodegmi
. In contrast to the very close relationship among them,
C. cynodegmi
and
Capnocytophaga canimorsus
differ dramatically in terms of virulence in humans. Comparative genomics provided some understanding on how
Capnocytophaga
species may switch from being dog commensals to human pathogens.
Draft Genome Sequence of Aneurinibacillus tyrosinisolvens LL-002
T
, Which Possesses Some Pseudouridine Synthases
Authors: Tsubouchi Taishi , Nishi Shinro , Maruyama Tadashi , Hatada Yuji 
Published: May 2015

ABSTRACT

We report the 5.7-Mb draft genome sequence of
Aneurinibacillus tyrosinisolvens
strain LL-002
T
, isolated from organic- and methane-rich sea sediments. The draft genome sequence of strain LL-002
T
consists of 5,693,818 bp in 136 contigs, with a G+C content of 44.5%, 5,946 potential coding sequences (CDS), 2 rRNAs, and 39 tRNAs.
Genome Sequence of Banana Streak MY Virus from the Pacific Ocean Island of Tonga
Authors: Stainton Daisy , Halafihi Mana’ia , Collings David A. , Varsani Arvind 
Published: May 2015

ABSTRACT
Banana streak disease is caused by a variety of banana-infecting badnaviruses. A genome of the episomal form of a banana streak MY virus was recovered from an infected banana plant sampled on Vava’u Island, Tonga, and shares >98% pairwise identity with the six other genomes available in public databases.
Genome Sequence of Lactobacillus curieae CCTCC M 2011381
T
, a Novel Producer of Gamma-aminobutyric Acid
Authors: Wang Ying , Wang Yu , Lang Chong , Wei Dongzhi , Xu Ping , Xie Jingli 
Published: May 2015

ABSTRACT

Lactobacillus curieae
CCTCC M 2011381
T
is a novel species of the genus
Lactobacillus
and a gamma-aminobutyric acid producer that was isolated from stinky tofu brine. Here, we present a 2.19-Mb assembly of its genome, which may provide further insights into the molecular mechanisms underlying its beneficial properties.
Complete Genome Sequence of Listeria monocytogenes N2306, a Strain Associated with the 2013-2014 Listeriosis Outbreak in Switzerland
Authors: Tasara Taurai , Ebner Rebecca , Klumpp Jochen , Stephan Roger 
Published: May 2015

ABSTRACT

We present the complete genome sequence of
Listeria monocytogenes
N2306, a serotype 4b clinical strain isolated during the 2013-2014 nationwide listeriosis outbreak in Switzerland.
Complete Genome Sequence of Corynebacterium camporealensis DSM 44610, Isolated from the Milk of a Manchega Sheep with Subclinical Mastitis
Authors: Rückert Christian , Albersmeier Andreas , Winkler Anika , Tauch Andreas 
Published: May 2015

ABSTRACT

Corynebacterium camporealensis
has been isolated in pure culture from milk samples of dairy sheep affected by subclinical mastitis. The complete genome sequence of the type strain DSM 44610, recovered from milk of a Manchega sheep, comprises 2,451,810 bp with a mean G+C content of 59.41% and 2,249 protein-coding genes.
Explaining variation in cancer survival between 11 jurisdictions in the International Cancer Benchmarking Partnership: a primary care vignette survey
Authors: Rose Peter W , Rubin Greg , Perera-Salazar Rafael , Almberg Sigrun Saur , Barisic Andriana , Dawes Martin , Grunfeld Eva , Hart Nigel , Neal Richard D , Pirotta Marie , Sisler Jeffrey , Konrad Gerald , Toftegaard Berit Skjødeberg , Thulesius Hans , Vedsted Peter , Young Jane , Hamilton Willie ,   The ICBP Module 3 Working Group*
Published: May 2015

ObjectivesThe International Cancer Benchmarking Partnership (ICBP) is a collaboration between 6 countries and 12 jurisdictions with similar primary care-led health services. This study investigates primary care physician (PCP) behaviour and systems that may contribute to the timeliness of investigating for cancer and subsequently, international survival differences.DesignA validated survey administered to PCPs via the internet set out in two parts: direct questions on primary care structure and practice relating to cancer diagnosis, and clinical vignettes, assessing management of scenarios relating to the diagnosis of lung, colorectal or ovarian cancer.Participants2795 PCPs in 11 jurisdictions: New South Wales and Victoria (Australia), British Columbia, Manitoba, Ontario (Canada), England, Northern Ireland, Wales (UK), Denmark, Norway and Sweden.Primary and secondary outcome measuresAnalysis compared the cumulative proportion of PCPs in each jurisdiction opting to investigate or refer at each phase for each vignette with 1-year survival, and conditional 5-year survival rates for the relevant cancer and jurisdiction. Logistic regression was used to explore whether PCP characteristics or system differences in each jurisdiction affected the readiness to investigate.Results4 of 5 vignettes showed a statistically significant correlation (p<0.05 or better) between readiness to investigate or refer to secondary care at the first phase of each vignette and cancer survival rates for that jurisdiction. No consistent associations were found between readiness to investigate and selected PCP demographics, practice or health system variables.ConclusionsWe demonstrate a correlation between the readiness of PCPs to investigate symptoms indicative of cancer and cancer survival rates, one of the first possible explanations for the variation in cancer survival between ICBP countries. No specific health system features consistently explained these findings. Some jurisdictions may consider lowering thresholds for PCPs to investigate for cancer—either directly, or by specialist referral, to improve outcomes.
A stochastic bioenergetics model-based approach to translating large river flow and temperature into fish population responses: the pallid sturgeon example
Authors: Wildhaber Mark L. , Dey Rima , Wikle Christopher K. , Moran Edward H. , Anderson Christopher J. , Franz Kristie J. 
Published: May 2015

Abstract

In managing fish populations, especially at-risk species, realistic mathematical models are needed to help predict population response to potential management actions in the context of environmental conditions and changing climate while effectively incorporating the stochastic nature of real world conditions. We provide a key component of such a model for the endangered pallid sturgeon (
Scaphirhynchus albus
) in the form of an individual-based bioenergetics model influenced not only by temperature but also by flow. This component is based on modification of a known individual-based bioenergetics model through incorporation of: the observed ontogenetic shift in pallid sturgeon diet from marcroinvertebrates to fish; the energetic costs of swimming under flowing-water conditions; and stochasticity. We provide an assessment of how differences in environmental conditions could potentially alter pallid sturgeon growth estimates, using observed temperature and velocity from channelized portions of the Lower Missouri River mainstem. We do this using separate relationships between the proportion of maximum consumption and fork length and swimming cost standard error estimates for fish captured above and below the Kansas River in the Lower Missouri River. Critical to our matching observed growth in the field with predicted growth based on observed environmental conditions was a two-step shift in diet from macroinvertebrates to fish.
Submerged shorelines and landscape features offshore of Mossel Bay, South Africa
Authors: Cawthra Hayley C. , Compton John S. , Fisher Erich C. , MacHutchon Michael R. , Marean Curtis W. 
Published: May 2015

Abstract
Coastal geomorphic systems have been studied widely to understand the responses of shorelines to fluctuating sea levels. Submerged shorelines, remnant of Pleistocene sea-level lowstands, are well preserved on the South African continental shelf. This paper describes work undertaken to better understand offshore coastal environments now submerged by high sea levels off the South African south coast near Mossel Bay, offshore of the Pinnacle Point archaeological locality. Multibeam bathymetry and side-scan sonar reveal evidence of past sea-level fluctuations and submerged coastal landscape features on the seabed. These results form the basis of an ongoing palaeoenvironmental reconstruction for this part of the shelf. We describe seven significant geomorphic features that show a submerged environment that differs significantly to the immediate adjacent coastal plain. However, these features are comparable to other stretches of the present South African shoreline that serve as modern analogues. We propose that features on the continental shelf primarily reflect geological substrate, gradients and Pleistocene sea-level fluctuations. Early modern humans were likely to have had a different set of resources to use in this Pleistocene landscape compared to those available along the presently exposed coast.
New DNA Methylation Markers for Pancreatic Cancer: Discovery, Tissue Validation, and Pilot Testing in Pancreatic Juice
Authors: Kisiel John B. , Raimondo Massimo , Taylor William R. , Yab Tracy C. , Mahoney Douglas W. , Sun Zhifu , Middha Sumit , Baheti Saurabh , Zou Hongzhi , Smyrk Thomas C. , Boardman Lisa A. , Petersen Gloria M. , Ahlquist David A. 
Published: May 2015

Abstract
Purpose: Discriminant markers for pancreatic cancer detection are needed. We sought to identify and validate methylated DNA markers for pancreatic cancer using next-generation sequencing unbiased by known targets.
Experimental Design: At a referral center, we conducted four sequential case–control studies: discovery, technical validation, biologic validation, and clinical piloting. Candidate markers were identified using variance-inflated logistic regression on reduced-representation bisulfite DNA sequencing results from matched pancreatic cancers, benign pancreas, and normal colon tissues. Markers were validated technically on replicate discovery study DNA and biologically on independent, matched, blinded tissues by methylation-specific PCR. Clinical testing of six methylation candidates and mutant KRAS was performed on secretin-stimulated pancreatic juice samples from 61 patients with pancreatic cancer, 22 with chronic pancreatitis, and 19 with normal pancreas on endoscopic ultrasound. Areas under receiver-operating characteristics curves (AUC) for markers were calculated.
Results: Sequencing identified &gt;500 differentially hyper-methylated regions. On independent tissues, AUC on 19 selected markers ranged between 0.73 and 0.97. Pancreatic juice AUC values for CD1D, KCNK12, CLEC11A, NDRG4, IKZF1, PKRCB, and KRAS were 0.92*, 0.88, 0.85, 0.85, 0.84, 0.83, and 0.75, respectively, for pancreatic cancer compared with normal pancreas and 0.92*, 0.73, 0.76, 0.85*, 0.73, 0.77, and 0.62 for pancreatic cancer compared with chronic pancreatitis (*, P = 0.001 vs. KRAS).
Conclusions: We identified and validated novel DNA methylation markers strongly associated with pancreatic cancer. On pilot testing in pancreatic juice, best markers (especially CD1D) highly discriminated pancreatic cases from controls. Clin Cancer Res; 21(19); 4473–81. ©2015 AACR.
PKCϵ Is an Essential Mediator of Prostate Cancer Bone Metastasis
Authors: Gutierrez-Uzquiza Alvaro , Lopez-Haber Cynthia , Jernigan Danielle L. , Fatatis Alessandro , Kazanietz Marcelo G. 
Published: May 2015

Abstract
The bone is a preferred site for metastatic homing of prostate cancer cells. Once prostate cancer patients develop skeletal metastases, they eventually succumb to the disease; therefore, it is imperative to identify key molecular drivers of this process. This study examines the involvement of protein kinase C epsilon (PKCϵ), an oncogenic protein that is abnormally overexpressed in human tumor specimens and cell lines, on prostate cancer cell bone metastasis. PC3-ML cells, a highly invasive prostate cancer PC3 derivative with bone metastatic colonization properties, failed to induce skeletal metastatic foci upon inoculation into nude mice when PKCϵ expression was silenced using shRNA. Interestingly, while PKCϵ depletion had only marginal effects on the proliferative, adhesive, and migratory capacities of PC3-ML cells in vitro or in the growth of xenografts upon s.c. inoculation, it caused a significant reduction in cell invasiveness. Notably, PKCϵ was required for transendothelial cell migration (TEM) as well as for the growth of PC3-ML cells in a bone biomimetic environment. At a mechanistic level, PKCϵ depletion abrogates the expression of IL1β, a cytokine implicated in skeletal metastasis. Taken together, PKCϵ is a key factor for driving the formation of bone metastasis by prostate cancer cells and is a potential therapeutic target for advanced stages of the disease.
Implications: This study uncovers an important new function of PKCϵ in the dissemination of cancer cells to the bone; thus, highlighting the promising potential of this oncogenic kinase as a therapeutic target for skeletal metastasis. Mol Cancer Res; 13(9); 1336–46. ©2015 AACR.
Deregulation of Retinaldehyde Dehydrogenase 2 Leads to Defective Angiogenic Function of Endothelial Colony–Forming Cells in Pediatric Moyamoya Disease
Authors: Lee Ji Yeoun , Moon Youn Joo , Lee Hae-Ock , Park Ae-Kyung , Choi Seung-Ah , Wang Kyu-Chang , Han Jung Woo , Joung Je-Gun , Kang Hyun Seung , Kim Jeong Eun , Phi Ji Hoon , Park Woong-Yang , Kim Seung-Ki 
Published: May 2015

Objective––
Moyamoya disease (MMD) is a common cause of childhood stroke, in which the abnormal function of the endothelial colony–forming cell (ECFC) plays a key role in the pathogenesis of the disease. This study was designed to identify genes involved in MMD pathogenesis using gene expression profiling and to understand the defective function of MMD ECFCs.

Approach and Results––

We compared gene expression profiles of ECFCs isolated from patients with MMD and normal controls. Among the differentially expressed genes, we selected a gene with the most downregulated expression, retinaldehyde dehydrogenase 2 (
RALDH2
). The activity of RALDH2 in MMD ECFCs was assessed by in vitro tube formation assay and in vivo Matrigel plug assay in the presence of all-trans retinoic acid. The transcriptional control of RALDH2 was tested using ChIP assays on acetyl-histone H3. In the results, MMD ECFCs inefficiently formed capillary tubes in vitro and capillaries in vivo, a defect restored by all-trans retinoic acid treatment. Knockdown of RALDH2 mRNA in normal ECFCs also induced decreased activity of capillary formation in vitro. The decreased level of RALDH2 mRNA in MMD ECFCs was attributed to defective acetyl-histone H3 binding to the promoter region.

Conclusions––
From these results, we conclude that the expression of RALDH2 was epigenetically suppressed in ECFCs from patients with MMD, which may play a key role in their functional impairment.
Epigenetic Regulation of Angiogenesis by JARID1B-Induced Repression of HOXA5
Authors: Fork Christian , Gu Lunda , Hitzel Juliane , Josipovic Ivana , Hu Jiong , SzeKa Wong Michael , Ponomareva Yuliya , Albert Mareike , Schmitz Sandra U. , Uchida Shizuka , Fleming Ingrid , Helin Kristian , Steinhilber Dieter , Leisegang Matthias S. , Brandes Ralf P. 
Published: May 2015

Objective—
Altering endothelial biology through epigenetic modifiers is an attractive novel concept, which is, however, just in its beginnings. We therefore set out to identify chromatin modifiers important for endothelial gene expression and contributing to angiogenesis.

Approach and Results—

To identify chromatin modifying enzymes in endothelial cells, histone demethylases were screened by microarray and polymerase chain reaction. The histone 3 lysine 4 demethylase JARID1B was identified as a highly expressed enzyme at the mRNA and protein levels. Knockdown of JARID1B by shRNA in human umbilical vein endothelial cells attenuated cell migration, angiogenic sprouting, and tube formation. Similarly, pharmacological inhibition and overexpression of a catalytic inactive JARID1B mutant reduced the angiogenic capacity of human umbilical vein endothelial cells. To identify the in vivo relevance of JARID1B in the vascular system,
Jarid1b
knockout mice were studied. As global knockout results in increased mortality and developmental defects, tamoxifen-inducible and endothelial-specific knockout mice were generated. Acute knockout of Jarid1b attenuated retinal angiogenesis and endothelial sprout outgrowth from aortic segments. To identify the underlying mechanism, a microarray experiment was performed, which led to the identification of the antiangiogenic transcription factor
HOXA5
to be suppressed by JARID1B. Importantly, downregulation or inhibition of JARID1B, but not of JARID1A and JARID1C, induced HOXA5 expression in human umbilical vein endothelial cells. Consistently, chromatin immunoprecipitation revealed that JARID1B occupies and reduces the histone 3 lysine 4 methylation levels at the
HOXA5
promoter, demonstrating a direct function of JARID1B in endothelial
HOXA5
gene regulation.

Conclusions—
JARID1B, by suppressing HOXA5, maintains the endothelial angiogenic capacity in a demethylase-dependent manner.