bluuebunny/crossref_metadata_2025_split huggingface dataset:
Generic residue numbering of the GAIN domain of adhesion GPCRs
Authors: Seufert Florian , Pérez-Hernández Guillermo , Pándy-Szekeres Gáspár , Guixà-González Ramon , Langenhan Tobias , Gloriam David E. , Hildebrand Peter W. 
Published: July 2024

Abstract

The GPCR autoproteolysis inducing (GAIN) domain is an ancient protein fold ubiquitous in adhesion G protein-coupled receptors (aGPCR). It contains a concealed tethered agonist element, which is necessary and sufficient for receptor activation. The GAIN domain is a hotspot for pathological mutations. However, the low primary sequence conservation of GAIN domains has thus far hindered the knowledge transfer across different GAIN domains in human receptors as well as species orthologs. Here, we present a scheme for generic residue numbering of GAIN domains based on structural alignments of six experimental and more than 14,000 modeled GAIN domain structures. This scheme is implemented in the GPCR database (GPCRdb) and elucidates the domain topology across different aGPCRs and their homologs in a large panel of species. We identify conservation hotspots and cancer-enriched positions in human aGPCRs and show the transferability of positional and structural information between GAIN domain homologs. The GAIN-GRN scheme provides a robust strategy to allocate structural homologies at the primary and secondary levels also to GAIN folds of GAIN domains of polycystic kidney disease 1/PKD1-like proteins, which now renders positions in both GAIN domain types comparable to one another.
Macro-to-nanoscale investigation unlocks gold and silver enrichment by lead-bismuth metallic melts in the Switchback epithermal deposit, southern Mexico
Authors: Cano Néstor , González-Jiménez José María , Camprubí Antoni , Proenza Joaquín A. , González-Partida Eduardo 
Published: July 2024

Abstract
Lead-bismuth (Pb-Bi) minerals of the galena (PbS)-matildite (AgBiS2) series and lillianite homologues (Pb3–2xAgxBi2+xS6) are intergrown with electrum (Au-Ag alloy) and chalcopyrite (CuFeS2) in specific bands within a colloform-banded vein at the Switchback epithermal deposit in southern Mexico. A macro-to-nano scale study revealed that these minerals fill small (&lt;200 µm) cavities in the gangue minerals, showing curvilinear boundaries, bleb-like morphologies, and rounded nanoparticles (~100 nm). These observations are consistent with growth in a molten system from a precursor Pb-Bi melt containing Au, Ag, and Cu. Minerals in the galena-matildite series typically display Widmanstätten textures (i.e., octahedral-like or basket-weave matilditess lamellae in the galenass matrix), which have been traditionally linked to the decomposition of a high-temperature solid solution. However, galenass and matilditess show nano-scale sinuous reaction fronts and replacement relicts (“islands”) while maintaining the [011]Galena || [100]Matildite crystallographic orientation relationship. This suggests a topotaxial growth of matilditess mediated by coupled dissolution-reprecipitation reactions between galenass and the metallic melt upon cooling. A similar scenario is proposed for Pb-Bi sulfosalt intergrowths, which replace galena-matildite and electrum and grow topotaxially along (200)Galena. Collectively, these results suggest that Pb-Bi melts can exist in epithermal fluids, acting as precursors for the crystallization of ore minerals and able to sequester precious metals. This model explains abnormally high Au-Ag enrichments observed in some deposits that contain Pb-Bi ores.
Risk Factors of Asymptomatic Kidney Stone Passage in Adults with Recurrent Kidney Stones
Authors: Stritt Kevin , Fuster Daniel G. , Dhayat Nasser A. , Bonny Olivier , Faller Nicolas , Christe Andreas , Taha Anas , Ochs Vincent , Ortlieb Niklas , Roth Beat ,   on behalf of the NOSTONE Study
Published: July 2024

Key Points

Asymptomatic stone passage was common in patients with recurrent calcium-containing kidney stones.Higher the number of stones at presentation, more likely it was that a kidney stone would spontaneously pass over time without causing any symptoms.

Background
Kidney stones are a common health problem and are characterized by a high risk of recurrence. A certain number of kidney stones pass asymptomatically. Data regarding the frequency of asymptomatic spontaneous stone passages are limited.

Methods
To assess the frequency of asymptomatic spontaneous stone passage and its covariates, we conducted a post hoc analysis of the prospective randomized NOSTONE trial. All asymptomatic spontaneous stone passages were identified by comparing the total number of kidney stones on low-dose noncontrast computed tomography (CT) imaging at the beginning and end of the study, considering symptomatic stone passages and surgical stone removal. The statistical analysis focused on the association of independent variables and the number of asymptomatic spontaneous stone passages using linear regression analyses.

Results
Of the 416 randomized patients, 383 with both baseline and end-of-study CT were included in this analysis. The median follow-up period was 35 months, the median patient age was 49 years (interquartile range [IQR], 40–55), and 20% of the patients were female. A total of 442 stone events occurred in 209 of 383 (55%) patients: 217 of 442 (49%) were symptomatic spontaneous stone passages, 67 of 442 (15%) were surgically removed stones, and 158 of 442 (36%) were asymptomatic spontaneous stone passages. The median size of asymptomatic stones (2.4 mm; IQR, 1.95–3.4) and the size of symptomatic stones (2.15 mm; IQR, 1.68–2.79) that passed spontaneously were not significantly different (P = 0.37). The number of asymptomatic spontaneous stone passages was significantly associated with a higher number of stones on CT at randomization (P = 0.001). Limitations include the lack of data on stone size at the time of passage and overrepresentation of White men.

Conclusions
Asymptomatic stone passage was common in patients with recurrent calcium-containing kidney stones. The higher the number of stones at presentation, the more likely it was that a kidney stone would spontaneously pass over time without causing any symptoms.

Clinical Trial registry name and registration number:
NOSTONE trial and: NCT03057431.
332-OR: Multiomic Profiling of Extended Honeymoon Unveils New Therapeutic Targets
Authors: LORETELLI CRISTIAN , GOUDA ABDELRAHMAN ABDELSALAM AHMED , BEN NASR MOUFIDA , USUELLI VERA , ASSI EMMA , ZOCCHI MONICA , PETRAZZUOLO ADRIANA , PETITTI AGNESE , CANNALIRE GIUSEPPE , D'ADDIO FRANCESCA , MAMELI CHIARA , FIORINA PAOLO 
Published: July 2024

Introduction & Objective: Approximately 50% of patients with new-onset Type 1 Diabetes (T1D) experience a temporary recovery in pancreatic β-cell function, termed the "honeymoon" (HM) phase, lasting approximately 7-9 months on average, and only rarely extending to years. This phase provides a crucial window for interventions to preserve insulin secretion, yet the factors contributing to its occurrence remain unclear. This study presents the first comprehensive multi-omic profiling of children with new-onset T1D in an extended honeymoon phase (ExMoon), revealing potential molecular targets for preserving β-cell mass and function.
Methods: Patients in ExMoon were defined by insulin dose-adjusted HbA1c (IDAA1c) below 9 and C peptide &gt;300 pmol/l, sustained for at least 9 months. We conducted analyses of PBMC immunophenotype, immunoreactivity to islet antigens, serum secretomics, proteomics/metabolomics/lipidomics, and PBMC transcriptomics using flow cytometry, ELISpot, immunomagnetic separation, mass spectrometry, and RNA sequencing, respectively. Profiles of ExMoon patients were compared to age- and gender-matched patients with T1D not in the HM phase (n=10 per group), with 10 matched nondiabetic patients included as additional controls.
Results: Differential serum levels of immune factors (IP-10, IL-2, FGF2), proteins (TGM2, SIR4), metabolites (kynurenine), and lipids (myristic acid and monoarachidonic acid triglyceride 18:0_38:6) were observed in ExMoon compared to the T1D group. PBMCs obtained from patients of the two groups exhibited distinct expression patterns of ERAP2, TSKS mRNAs, and of miR-339-3p, miR-8087-3p miRNAs. No differences were found in the proportion of immune cell subpopulations and islet autoreactivity between the ExMoon and T1D patient cohorts.
Conclusion: Our unbiased multiomic approach identified several immune and non-immune factors as potential molecular candidates for targeted therapies aimed at preserving β-cell mass and function.

Disclosure
C. Loretelli: None. A. Gouda Abdelrahman Abdelsalam: None. M. Ben Nasr: Research Support; Altheia Sciences. V. Usuelli: None. E. Assi: None. M. Zocchi: None. A. Petrazzuolo: None. A. Petitti: None. G. Cannalire: None. F. D'Addio: None. C. Mameli: None. P. Fiorina: None.

Funding
Fondazione "Romeo ed Enrica Invernizzi"
Identification of Potentially Inappropriate Medication in Geriatric Patients at Wangaya Hospital, Denpasar City with Beers Criteria and Stopp Criteria in 2023
Authors: Putri Made Shinta Sanma , Prasetya Anak Agung Ngurah Putra Riana , Kurnianta Putu Dian Marani 
Published: July 2024

Geriatric (elderly) patients generally require a number of drugs (polypharmacy) to cure health-related conditions that tend to occur Potentially Inappropriate Medication (PIM). Identification of PIM is very important to prevent Drug Related Problems (DRP). The purpose of the study was to identify the incidence of PIM using the Beers Criteria and STOPP Criteria and analyze the relationship between PIM risk factors and the incidence of PIM in geriatric patients at Wangaya Hospital, Denpasar City. This type of research is retrospective observational in a cross-sectional manner using simple random sampling techniques on all geriatric patients for the period 2023. Samples that met the inclusion criteria, namely geriatric patients aged ≥65 years in outpatient and inpatient facilities with geriatric patients who died and who were referred to other hospitals, were excluded from this study. Of the 354 samples, the prevalence of PIM in outpatient care was (4.23%) with the most common drugs being spironolactone (55.84%), Sulfonylureas (19.48%), and NSAIDs (6.49%) with Beers criteria. In hospitalization, (0.84%) with NSAIDs (25%), corticosteroids (25%), clopidogrel (25%), and antipsychotics (25%) with STOPP criteria. Based on the evaluation results, there was a very weak correlation between the number of drugs (p=0.000; r=0.199) and there was no relationship between the number of diagnoses (p=0.674) prescribed and the incidence of PIM. Based on this, it can be concluded that the greater the number of diseases and the number of drugs can increase the risk of PIM.
Pengembangan Cultural Tourism pada Kampung Wisata Melalui Branding dan Pemasaran digital
Authors: Kussudyarsana K , Widi Utami Dyah , Halim  
Published: July 2024

Pengabdian Masyarakat ini bertujuan untuk mengembangkan wisata budaya (cultural tourism) di Kampung Kemlayan, Kecamatan Serengan, Kotamadya Surakarta. Kemlayan selama ini dikenal sebagai tempat banyak maestro budaya lahir, namun mengalami degradasi sebagai dampak dari aktivitas ekonomi yang terjadi di daerah tersebut. Kegiatan pengabdian masyarakat diharapkan dapat mengembalikan lagi citra Kemlayan sebagai Kampung Budaya. Terdapat empat strategi utama utama yang diterapkan dalam PKM pengembangan wisata budaya Kampung Kemlayan. Pertama, ditetapkanlah jalur wisata Kampung Kemlayan dengan empat tema utama: sejarah, budaya, maestro, dan kuliner. Kedua, dilakukan kegiatan branding melalui pembuatan logo, maskot, dan simbol. Aspek ketiga mencakup pembuatan strategi pemasaran digital wisata Kampung Kemlayan melalui platform sosial media seperti TikTok, YouTube, dan Instagram. Keempat, dilakukan pelatihan pengelolaan media sosial kepada masyarakat setempat. Hasil dari kegiatan ini telah di rasakan manfaatnya oleh masyarakat sekitar, khususnya mitra PKM yaitu LPMK Kemlayan yang tercermin pada kepuasan mereka atas kegiatan pengabdian masyarakat ini yang angkanya di atas 80%.
Intervensi Head Up 15-30 Derajat Terhadap Perfusi Serebral Pasien Traumatic Brain Injury: Studi Kasus
Authors: Saputra La Ode Agustino , Hafid Muh. Anwar , Jamaluddin Ahmad 
Published: July 2024

Latar Belakang: cedera kepala atau traumatic brain injury (TBI) merupakan suatu gangguan yang bersifat traumatik pada fungsi otak baik disertai dengan pendarahan maupun tanpa perdarahan dalam jaringan otak. Kejadian trauma kepala diperkirakan mencapai 500.000 kasus tiap tahunnya dengan angka kematian lebih dari 10% pasien meninggal sebelum tiba di rumah sakit dan lebih dari 100.000 pasien mengalami berbagai kecacatan. Fenomena yang ditemukan selama di lapangan bahwa pasien cedera kepala akibat kecelakaan lalu lintas yang dirawat di ruangan ICU Rumah Sakit Labuang Baji Makassar, mengalami gangguan perfusi serebral, penurunan kesadaran dan beberapa pasien mengalami nyeri kepala sebagai dampak dari ketidakefektifan perfusi serebral. Penanganan utama pada pasien cedera kepala adalah peningkatan status O2 dan memposisikan pasien dengan head up 15-300. Metode: metode penelitian yang digunakan ialah metode studi kasus dengan melakukan proses keperawatan dimulai dari pengkajian sampai evaluasi pada kasus traumatic brain injury di ruangan Intensive Care Unit (ICU) rumah sakit Labuang Baji Makassar. Hasil: Diagnosis keperawatan yang ditemukan ialah risiko perfusi jaringan serebral tidak efektif, gangguan mobilitas fisik, risiko defisit nutrisi, dan risiko infeksi. Pembahasan: intervensi head up 15-300 dapat melancarkan perfusi serebral dan menurunkan tekanan intrakranial melalui beberapa mekanisme, yakni menurunkan tekanan darah, perubahan ventilasi, meningkatkan aliran vena melalui vena jugularis yang tak berkatup, sehingga menurunkan volume darah vena sentral yang menurunkan tekanan intrakranial. Perpindahan CSS dari kompartemen intrakranial ke rongga subarachnoid spinal1 dapat menurunkan tekanan intrakranial dan memperbaiki perfusi serebral. Kesimpulan: intervensi head up 15-300 efektif untuk mengatasi masalah risiko perfusi serebral tidak efektif.
The Overview of Nutritional Status, Physical Activity, Menstrual Patterns, and Washing Behavior on teenagers
Authors: Bahar Hartati , Nur Hasanah Indah , Lestari Hariati 
Published: July 2024

Anemia is a common health problem in Indonesia. One-third of teenagers, or three to four out of ten teenagers, suffer from anemia. The aim of this research was to determine the nutritional health picture of adolescent girls at SMPN 12 Kendari based on their level of exercise, menstrual cycle, and habit of washing their hands with soap (CTPS). The population in this study was 42 grade 9 students. The sampling technique was simple random sampling with a research sample of 30 young women. Questionnaires are used to collect data to measure respondents' attitudes and behavior. The results showed that of the 30 respondents whose data were taken, there were 15 respondents who had a thin nutritional status (50%), there were 20 respondents who did light exercise (66.7%), there were 24 respondents with normal menstrual patterns (80%), and There were 21 respondents who had hand washing behavior that met the requirements (65.6%). It is hoped that young women will pay more attention to their daily consumption patterns so that they comply with balanced nutrition to improve their nutritional status.
Analisis Masalah Kesehatan di Kota Bandar Lampung, Malaria Tetap Menjadi Masalah Utama
Authors: Nasyaroeka Ayu Eka Buana Dewi , Sulistyawati Sulistyawati , Kurniawan Medi 
Published: July 2024

The Indonesian government wants to eradicate malaria in 2030. This suggests that throughout the previous three years, no native malaria cases had been discovered across the nation. It follows that every region needs to keep an eye on the malaria condition in its own territory. One of the areas with endemic malaria is Lampung Province. The purpose of this study is to analyze health issues and determine whether malaria is still a concern in Bandar Lampung City. USG was used to calculate the problem priority. Results, With an 80, malaria was found to be the main health problem. In conclusion, Bandar Lampung City's greatest challenge is still malaria.

Keywords : Malaria Control, USG, Lampung, vector control, public health problem
Optimasi Ukuran Kulit Mangga pada Ekoenzim dengan Sumber Karbon Molase
Authors: Shalihah Nuha Fathinah , Firlianti Sekar Putri , Hardhaneszha Azkaifa Syawqie , Djuarsa Peristiwati 
Published: July 2024

Latar Belakang: Ekoenzim merupakan salah satu hasil proses fermentasi yang memiliki banyak manfaat dengan memanfaat sampah organik seperti kulit mangga. Tujuan: Penelitian ini bertujuan untuk mengetahui perbedaan karakteristik ekoenzim berbahan limbah kulit mangga dengan perbedaan ukuran kulit mangga yang akan difermentasi. Metode: Penelitian ini dilakukan tiga tahapan; fermentasi pembuatan ekoenzim, uji organoleptik, serta uji kuantitatif. Variasi ukuran kulit mangga yang digunakan mulai dari ukuran kecil (1x2 cm), ukuran sedang (2x4 cm), dan ukuran besar (4x8 cm). Uji organoleptik dilakukan kepada 10 panelis dengan parameter yang sudah ditentukan, uji ini mencakup uji aroma dan uji warna ekoenzim. Uji kuantitatif dilakukan dengan mengukur pH ekoenzim menggunakan pH meter. Seluruh pengujian dilakukan pada 3 perlakuan eco-enzyme yang berbeda dengan 3 kali pengulangan, eco-enzyme tersebut di panen pada hari ke-35. Hasil: Pada uji organoleptik didapatkan bahwa ekoenzim dengan ukuran kulit mangga terkecil (1x2 cm) memiliki nilai yang lebih tinggi dari segi aroma juga warna dibandingkan dengan ekoenzim dengan ukuran yang lebih besar. Sedangkan pada uji kuantitatif didapatkan pH ekoenzim dengan ukuran kulit 1x2 cm memiliki pH paling asam. Dalam pembuatan ekoenzim kali ini juga didapatkan jamur pitera yang bermanfaat bagi kulit. Kesimpulan: Ukuran kulit mangga yang digunakan pada mini riset ini didapatkan ekoenzim paling baik yaitu ukuran terkecil (1x2 cm) menghasilkan nilai pH paling asam, nilai aroma terbaik yaitu 2,6 dan nilai warna terbaik yaitu 2,7.
ANALISIS BUTIR SOAL ULANGAN MATEMATIKA SEMESTER GENAP KELAS XI SMA NEGERI 2 PULAU MAYA
Authors: Usman Usman , Wiwid Wiwid , Sulisti Hidayu 
Published: July 2024

Penelitian ini bertujuan untuk mengetahui kualitas butir soal ulangan matematika semester genap kelas XI SMA Negeri 2 Pulau Maya tahun ajaran 2021/2022. Kualitas butir soal tersebut dapat dilihat melalui perhitungan validitas, reliabilitas, tingkat kesukaran dan daya pembeda. Penelitian ini menggunakan metode deskriptif kuantitatif. Sampel penelitian ini yaitu 54 siswa kelas XI SMA Negeri 2 Pulau Maya tahun ajaran 2021/2022. Dari hasil analisis validasi terdapat 3 soal yang tidak valid dan 17 soal yang valid. Sedangkan untuk analisis reliabilitas bisa dikatakan reliabel karena sudah memenuhi kriteria yang ditentukan. Terdapat 18 soal yang tingkat kesukarannya sedang dan 2 soal yang tingkat kesukarannya mudah pada analisis tingkat kesukaran. Pada analisis daya pembeda terdapat 5 soal dengan kriteria jelek, 9 soal dengan kriteria sangat baik, 5 soal dengan kriteria cukup dan 1 soal dengan kriteria baik. Berdasarkan analisis yang dilakukan mengenai 20 butir soal tersebut melalui validitas, reliabilitas, tingkat kesukaran dan daya pembeda, terdapat 17 soal yang memenuhi kriteria layak dipakai dan terdapat 3 soal yang harus direvisi.
MEDIA PEMBELAJARAN ELIMINASI GAUSS PADA MATRIKS ORDO 3X3 BERBASIS MATLAB
Authors: Ramadhania Rizki , Hairunisa Dheah , Havizul Havizul 
Published: July 2024

Penelitian ini bertujuan untuk meminimalisir dan menyelesaikan masalah mahasiswa dalam pembelajaran Eliminasi Gauss pada Matriks ordo 3x3. Penelitian ini merupakan penelitian pengembangan (R&D). Model pengembangan yang digunakan adalah model 4-D, yaitu Define, Design, Develop, dan Disseminate. Hasil survey masalah yang telah dilakukan melalui kuesioner online menunjukkan sebanyak rata-rata 61,9% dari 21 mahasiswa menghadapi beberapa masalah dalam pembelajaran Eliminasi Gauss, yaitu sering keliru dalam melakukan perhitungan di salah satu atau beberapa langkah penyelesaian soal Matriks 3x3 dengan metode Eliminasi Gauss, lupa langkah selanjutnya pada saat mengerjakan, dan sering lupa materi ketika tidak diulang kembali. Bedasarkan hasil analisis masalah tersebut, maka dikembangkan media pembelajaran Eliminasi Gauss berbasis MatLab. Hasil evaluasi satu-satu oleh ahli materi dan ahli media, mendapatkan kriteria yang layak digunakan. Hasil evaluasi kelompok kecil menunjukkan bahwa mayoritas mahasiswa terbantu dalam pembelajaran Eliminasi Gauss. Untuk pengembangan lebih lanjut media pembelajaran ini dapat ditambahkan fungsi untuk menghitung soal Matriks dengan selain metode Eliminasi Gauss seperti metode Eliminasi Gauss Jordan, aturan Cramer dan metode Substitusi.
Dog owners’ perceptions and veterinary-related decisions pertaining to changes in their dog’s behavior that could indicate pain
Authors: Kogan Lori R. , Currin-McCulloch Jen , Brown Emma , Hellyer Peter 
Published: July 2024

Abstract

OBJECTIVE
Assess dog owners’ concern levels and veterinary care decisions pertaining to behaviors that might signify pain before and after receiving brief pain-related educational information. Additionally, evaluate dog owners’ perceptions of a modified version of the Colorado State University Canine Acute Pain Scale to identify their dog’s pain level, determine whether they should take their dog to the veterinarian, and describe their dog’s pain to their veterinarian.

SAMPLE
367 survey respondents who resided in the US and were the primary caretaker of at least 1 dog.

METHODS
An electronic survey was created in Qualtrics and distributed via Prolific. Data were analyzed.

RESULTS
When assessing differences between participants’ concern levels before and after educational pain-related information, significant differences were found for 2 of the 3 scenarios. Significant differences were also found between participants’ veterinary-related behaviors before and after educational pain-related information for all 3 scenarios. The most common reasons for not seeking immediate veterinary care included elements related to cost, convenience, and dog-related stress during veterinary visits. Most participants reported finding the Canine Acute Pain Scale useful for identifying their dog’s pain level (89%), determining whether they should take their dog to the veterinarian (84%), and describing their dog’s pain to their veterinarian (89%).

CLINICAL RELEVANCE
Owners’ interpretations of and subsequent actions (or inactions) to behaviors that might suggest pain carry great implications for companion animals’ overall well-being. Following exposure to brief educational material about signs and symptoms of canine pain, owners in our study reported significant increases in stated concern related to their dog’s change in behavior and intention to seek veterinary care. These findings suggest that even very brief pain-related educational material can have a positive impact on owners’ perceptions of canine behavioral changes and increase the possibility that they will seek veterinary care.