bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zfp521 prolonged expression partially relaunches the generic neuronal genes in U87MG glioblastoma cells: a primitive study
Authors: Rahimi-Movaghar Vafa , Mirsadeghi Sara , Heidary Yasaman , Alizadeh Seyed Danial , Ghodsi Zahra , Harrop James , Ghodsi Seyed Mohammad , Kiani Sahar 
Published: June 2024

Abstract

Study Design: An experimental in-vitroand in-vivo parallel group study.
Objectives: To investigate the prolonged effects of Zfp521 on gene expression in the U87MG glioma cell line and assess its in vivo impact on animal movement post-transplantation into spinal cord injury (SCI).
Setting: Royan Institute for Stem Cell Biology and Technology Laboratory.
Methods: U87MG cells were transduced with Zfp521-IRES-GFP and maintained in neural inductive medium for over 3 weeks. Gene expression of Gfap, Itga6, Pax6, nestin, Sox1, Tubb3, and Olig2 was analyzed. Transplanted cells' impact on locomotor capacity in SCI was assessed using the Basso-Beattie-Bresnahan (BBB) scale and footprint analysis.
Results: Zfp521 overexpression induced morphological changes and aggregated formation in U87MG cells, with a transfection rate of 26%. Significant upregulation of Pax6, Tubb3, and Olig2 and decreasing of Sox1 were observed, while Gfap, Itga6, and nestin showed non-significant changes. In SCI animals, U87-Zfp521 exhibited substantial recovery in hindlimb motor coordination (BBB score of 12) and weight support. Moreover, gait analysis revealed increased step length, stride angle, and step width in U87-Zfp521 animalsduring a five-week treatment. While plantar application showed no significant improvement.
Conclusions: Controlling Zfp521 expression level prominently enables the neuronal and oligodendrocyte lineage alley in the glioblastoma cell line that can be the potential therapy for promoting recovery in GBM and SCIs, highlighting its role as a promising target for further exploration in neural regeneration strategies.
Sponsorship: This work was funded by Tehran University of Medical Sciences with grant number 97-02-38-39408.
Differentiating between PCNSL GCB Subtype and Non-GCB Subtype using Radiomics: A Multicenter Study
Authors: Shen Yelong , Wu Siyu , Wu Yanan , Cui Chao , Li Haiou , Yang Shuang , Liu Xuejun , Chen Xingzhi , Huang Chencui , Wang Ximing 
Published: June 2024

Abstract

Objectives
To investigate the characteristics and pathological basis of MRI in germinal center B cell (GCB) and non-germinal center B cell (non-GCB) in PCNSL (primary central nervous system lymphoma). And to explore the predictive ability of MRI radiomics-based in differentiating the GCB and non-GCB of PCNSL.
Methods
This study retrospectively analyzed standard diagnostic MRI examinations in 24 immunocompetent patients (9 men; age 56.4 ± 15.1 years) with GCB and 56 immunocompetent patients (35 men; age 61.1 ± 9.3 years) with non-GCB. The radiomics features were extracted from ADC, DWI, and T1-CE images respectively, and the features were screened by machine learning algorithm and statistical method. Finally, radiomics models of seven different sequence permutations were constructed. The area under the receiver operating characteristic (ROC AUC) curve was used to evaluate the predictive performance of all models. Delong test was utilized to compare the differences among models.
Results
The GCB cases all showed diffusion restriction, which was observed in 80.36% of the non-GBM cases; p < 0.05. Grade 3 edema was rare in GCB cases (8.33%) and common in non-GCB cases (50.00%); p < 0.001. 62.50% of male patients were non-GCB and 37.50% of female patients were non-GCB; p < 0.05. Additionally, patients with the GCB subtype are younger than those with the non-GCB subtype; p < 0.05. The best prediction model in our study used a combination of ADC, DWI, and T1-CE achieving the highest AUC of 0.854. And there was a significant difference between the best-combined model and some of the other models.
Conclusion
The GCB subtype is commonly seen in women, with mild peritumoral edema in most cases and diffusion restriction in all cases; however, the non-GCB subtype is commonly seen in men, with severe peritumoral edema in most cases. Additionally, the radiomics model developed by all sequences combined had good performance in discriminating between GCB and non-GCB.
Transcriptome of Tomato (Solanum lycopersicum L.) Reveals Impacts of Rhizosphere Microbiomes on Host Interactions
Authors: Garcia Joshua , Moravek Micaela , Fish Tara , Thannhauser Ted , Fei Zhangjun , Sparks Jed , Giovannoni James , Kao-Kniffin Jenny 
Published: June 2024

Abstract

The gut microbiome of worms from composting facilities potentially harbors organisms that are beneficial to plant growth and development. We transferred microbiomes from worm castings (i.e. vermicompost) into tomato (Solanum lycopersicum L.) rhizospheres to examine their impacts on plant growth and physiology. We hypothesized that tomatoes grown with living vermicompost microbiomes in their rhizospheres would display transcriptome changes in key nutrient pathways compared to sterilized vermicompost and control (no compost) inoculant treatments. Vermicompost microbiome treatment significantly impacted bacterial communities in tomato rhizospheres, but showed high fungal variability between treatments. Plants treated with vermicompost or sterile vermicompost exhibited increased productivity and foliar δ15N values at harvest, along with decreased fruit phytoene content at the red ripe stage, compared to the control. RNA sequencing revealed distinct gene expression changes in the vermicompost treatment, including upregulation of nutrient transporter genes. Despite phenotypic similarities between vermicompost and sterile vermicompost treatments, plant transcriptome analyses suggest that rhizosphere microbiomes derived from vermicompost can influence tomato gene expression and growth-related regulatory pathways. These findings highlight the potential of rhizosphere microbiomes to influence crops at the molecular level and the value of RNA sequencing in uncovering molecular responses in plant microbiome studies.
Stakeholder analysis of banning unhealthy product advertisement
Authors: Najafi Marziyeh , Mosadeghrad AliMohammad , Arab Mohammad 
Published: June 2024

Abstract

Background: The advertising of unhealthy products, by influencing the level of consumption of these products, has become a pressing concern due to its contribution to the rise of non-communicable diseases in society. Policy-making to control advertising, however, faces numerous challenges due to the need to involve different stakeholders. This study aims to analyze the stakeholders involved in banning the advertising of unhealthy products in Iran, potentially paving the way for more effective health policies.
Methods: This research was conducted through semi-structured interviews with 18 stakeholders in the advertising of unhealthy goods in Iran in 2022. The experts were selected using a purposive and snowball sampling method. PolicyMaker V.4 and MAXQDA V.16 software were used to analyze stakeholders.
Results: The study revealed that out of 26 stakeholders, 20 were active, and six were inactive. Notably, the media emerged as key stakeholders with significant power, opposing the ban on advertising harmful goods. On the other hand, the Ministry of Health, the National Standards Organisation, the Food and Drug Organisation, and the medical system were the most supportive actors. The Ministries of Culture and Industry and political institutions, while powerful, showed moderate support. These findings underscore the complex dynamics of stakeholder involvement in banning unhealthy product advertisements in Iran.
Conclusions: The study's findings highlight the crucial role of stakeholders with a neutral approach and high power in the success of banning unhealthy product advertisements. Importantly, the Ministry of Health, if granted more power to lead and manage stakeholders, could significantly enhance the effectiveness of health policies. This suggests that engaging influential stakeholders in a leadership team could be a promising strategy for future health policy initiatives.
Risk Factors for Acute Respiratory Distress Syndrome in Sepsis Patients: A Meta-Analysis
Authors: Yin Rui , Yang Xiaoshan , Yao yaoyanfen 
Published: June 2024

Abstract

Background
Acute Respiratory Distress Syndrome (ARDS) is a critical complication of sepsis, associated with high morbidity and mortality. Identifying risk factors for ARDS among sepsis patients is essential for early intervention and improving outcomes.
Methods
We conducted a comprehensive meta-analysis, reviewing studies that examined the association between various risk factors and ARDS development in sepsis patients. Databases such as PubMed, EMBASE, Cochrane Library, Medline, CINAHL, and Web of Science were searched up to January 2024, without language restrictions. Eligible studies included observational cohorts and case-control studies. Pooled odds ratios (ORs) and standardized mean differences (SMDs) were calculated using a random-effects model. Heterogeneity was assessed through I² statistics, and publication bias was evaluated via the Luis Furuya-Kanamori (LFK) index.
Results
15 studies with more than 40,000 participants were analyzed. Significant risk factors for ARDS included pulmonary infection (OR: 2.696, 95% CI: 1.655 to 4.390), septic shock (OR: 2.627, 95% CI: 1.850 to 3.731), and pancreatitis (OR: 3.734, 95% CI: 2.958 to 4.712). No significant associations were found for sex (OR: 1.106, 95% CI: 0.957 to 1.279), smoking status (OR: 1.214, 95% CI: 0.835 to 1.765), or steroid use (OR: 0.901, 95% CI: 0.617 to 1.314). APACHE-II and SOFA scores were predictive of ARDS development, emphasizing their utility in clinical assessments.
Conclusion
Pulmonary infection, septic shock, and pancreatitis significantly increase ARDS risk in sepsis patients. Our findings advocate for targeted management of these risk factors to mitigate ARDS development, emphasizing the importance of personalized care in sepsis management.
Assessment of Molecular Modulation by Multifrequency Electromagnetic Pulses to Preferably Eradicate Tumorigenic cells
Authors: Piredda Roberta , Martínez Luis G. Rodríguez , Stakamakis kostantinos , Martinez-Ortega Jorge , Ferraz Alejandro López , Almendral Jose M. , Revilla Yolanda 
Published: June 2024

Abstract

Physics methods of cancer therapy are extensively used in the clinical practice, but they are invasive and often confront undesired side effects. A fully new equipment that allows sustained emission of intense and time controlled non-ionizing multifrequency electromagnetic pulse (MEMP), has been applied to eukaryotic cells in culture. The equipment discriminates the overall electronegative charge of the cell cultures, and its subsequent proportional emission may thereby become higher and lethal to cancer cells of generally high metabolic activity, whereas low tumorigenic cells would be much less affected. We tested the specificity and efficacy of the equipment against a collection of (i) highly tumorigenic cells of human (glioblastoma, cervical carcinoma, and skin) and mouse (colon adenocarcinoma) origin; (ii) cell lines of much lower tumorigenicity (non-human primate kidney and mouse fibroblasts), and (iii) primary porcine macrophages lacking tumorigenicity. Time and intensity control of the MEMP allowed progressive decay of viability fairly correlating to cell tumorigenicity, which was provoked by a proportional alteration of the cytoplasmic membrane permeability, cell cycle arrest at G2, and general collapse of the actin and intermediate filaments cytoskeleton to the perinuclear region. Correspondingly, these effects drastically inhibited the proliferative capacity of the most tumorigenic cells in clonogenic assays. Moreover, MEMP suppressed in a dose-dependent manner the tumorigenicity of retrovirally transduced luciferase expressing colon adenocarcinoma cells in xenografted immune-competent mice, as determined by tumor growth in a bioluminescence imaging system. Our results support MEMP as an anti-cancer non-invasive physical treatment of substantial specificity for tumorigenic cells with promising therapeutic potential in oncology.
Effect of Different Fixed Lingual Retainers on Tooth Mobility: Prospective Clinical Study
Authors: KÜÇÜK Eyüp Burak , GÜLER Özge ÇELİK , ARPAĞ Osman Fatih , EREN Hatice Kübra 
Published: June 2024

Abstract

Objective: The aim of this cross-sectional study was to compare the effect of different fixed lingual retainers (LRs) on tooth mobility after orthodontic treatment using a Periotest® device.
Materials and methods: A total of 82 patients (43 females and 39 males; mean age = 18.61 ± 3.71 years) with no missing teeth in the lower inter-canine region were treated with fixed orthodontic mechanics. At the completion of the orthodontic treatment, each patient was fitted with either Ortho FlexTech (Reliance; Group I), dead soft 8-braided (Bond-A-Braid, Reliance; Group II), or 5-stranded (PentaOne, Masel; Group III) LR wire. A control group consisting of people with a healthy periodontal condition and no bone loss was included (Control; Group C). The mobility of the lower anterior teeth on the right and left sides was measured using a Periotest® device before (T0), immediately after (T1), and 10 months after (T2) the application of the LR. Statistical analyses were performed using one-way ANOVA followed by post hoc LSD, repeated ANOVA with Bonferroni post hoc test, and Pearson χ2 tests.
Results: Tooth mobility was significantly lower at T1 and T2 compared to that at T0 (P < 0.05); however, there were no differences in the mobility at T1 and T2 in all study groups (P > 0.05). When the mobility measurements taken at the different times were compared, there was no statistical difference between the groups (P > 0.05).
Conclusions: In all the study groups, tooth mobility decreased immediately after LR application and did not change during the 10-month follow-up. There were no significant differences in tooth mobility among the study groups.
Clinical relevance: Any of the studied types of retainer wires could be used by clinicians.
Clinical Trial Number: NCT06284499
Genomic profiling of lymph node and distant metastases from papillary and poorly differentiated thyroid carcinomas
Authors: Máximo Valdemar , Melo Miguel , Sobrinho-Simões Manuel , Soares Paula , Paula Arnaud Da Cruz 
Published: June 2024

Abstract

Purpose: To perform a molecular profiling of the metastases from papillary thyroid carcinomas (PTCs) and poorly differentiated thyroid carcinomas (PDTCs).
Methods: We retrieved and analyzed the molecular and clinical features of 136 metastases from PTCs and 35 metastases from PDTCs subjected to targeted DNA sequencing, from cBioPortal. The clinicopathological data included the number and location of the metastases, and genomic data included mutations, translocations, copy number alterations and fraction of the genome altered (FGA).
Results: Bone metastases from PTCs had a lower frequency of BRAF mutations than the lymph node metastases (LNMs) (43% vs 88%, p<0.01), and a higher frequency of RBM10 and NRAS mutations than the LNMs (21% vs 3% for both, p<0.05). The FGA of the bone metastases was higher than the FGA of the lung metastases (5.6% vs 1.3%, p<0.05). The frequency of RETtranslocations was higher in the lung metastases from PTCs than the LNMs (15% vs 3%, p<0.05). The LNMs from PTC patients harboring 4 or more distant metastases (DMs) had a higher frequency of TERTpromoter mutations than the LNMs from patients harboring less than 4 DMs (96% vs 65%, p<0.001). SDHA gene amplifications were enriched in the bone metastases from PDTCs and absent in the LNMs (38% vs 0%, p<0.05). Conclusion: Metastases from PTCs and PDTCs harbor clinically relevant alterations affecting distinct body locations, such as NRAS and RBM10 mutations, RET translocations and SDHAamplifications that may be explored therapeutically.
Genetic association of allergic diseases, serum IgE levels and osteoarthritis: a Mendelian randomization study
Authors: Zhang Kai , Xiong Xianghua , Wu Ning , Li Hangyu 
Published: June 2024

Abstract

Objective
The question of whether there is a causal relationship between allergic diseases, immunoglobulin E levels, and osteoarthritis remains unclear. This study aimed to examine the bidirectional causal association between allergic diseases, serum IgE levels, and osteoarthritis using a Mendelian randomization study approach.
Methods
This study utilized publicly available pooled statistics from large genome-wide association studies to assess the causal associations between allergic diseases, serum IgE levels, and osteoarthritis. The analysis employed inverse variance weighting, MR-Egger, weighted median, and weighted mode methods. Sensitivity analyses were also conducted using MR-Egger regression, Cochran's Q test, and MR-PRESSO test to ensure the reliability of the findings.
Results
The IVW results indicated that there was no significant causal relationship between allergic asthma and atopic dermatitis with KOA (OR = 1.02, 95% CI:0.96–1.09, P = 0.544; OR = 1.00, 95% CI:0.96–1.04, P = 0.933) and HOA (OR = 0.95, 95% CI:0.89–1.02, P = 0.127; OR = 1.00, 95% CI: 0.94–1.06, P = 0.992). Similarly, KOA and HOA did not have a causal effect on allergic asthma (OR = 1.07, 95% CI: 0.86–1.32, P = 0.553; OR = 1.04, 95% CI: 0.89–1.23, P = 0.610), atopic dermatitis (OR = 1.05, 95% CI: 0.83–1.31, P = 0.692; OR = 0.94, 95% CI: 0.83–1.05, P = 0.284), and serum IgE levels (OR 0.98, 95% CI: 0.74–1.31, P = 0.916; OR 1.15, 95% CI: 0.99–1.34, P = 0.071). However, there was a causal effect of serum IgE levels on KOA, as they were found to increase the incidence of KOA (OR = 1.05, 95% CI: 1.00-1.10, P < 0.05). On the other hand, serum IgE levels did not have a causal effect on HOA (OR = 1.01, 95% CI: 0.94–1.08, P = 0.799).
Conclusions
From a genetic perspective, a causal relationship between serum IgE levels and the risk of KOA was observed. However, there was no evidence of a bidirectional causality between atopic disease and OA. Furthermore, the use of MR methods did not support the existence of a causal effect of IgE levels on HOA or OA on IgE levels.
Modeling Daily Profiles of ER and Socialization While Caregiving: Links to Regulation Success and Child Emotional Traits
Authors: Cox Margaret N. , Weston Sara J. , Garcia Jazz , Wright Joanna H. , Gallo John C.R. , Giuliani Nicole R. 
Published: June 2024

Abstract

There is a well-established effect of caregiver responses to child emotions on child emotion regulation development. However, targeted family interventions require knowledge of how caregivers regulate their own (emotion regulation; ER) and their child’s (emotion socialization strategies) emotions during daily parent-child interactions, and how contextual variables influence perceived ER and socialization success. This study uses ecological momentary assessment methods (Nparticipants = 197; 3 surveys per day over 7 days) and latent class analysis to model daily ER and socialization strategies used by caregivers of preschool-aged children in daily life. Results indicated that perceived regulation success was highest for caregivers in ER and socialization profiles characterized by frequent endorsement of “no regulation.” However, ER and socialization profiles characterized by high diversity (e.g., flexibility) in strategies were most successful for regulating the emotions of children with high emotional lability and for regulating caregiver emotions at timepoints when caregivers endorsed experiencing a negative emotion, respectively. Thus, results of this study suggest that competence with diverse ER and socialization strategies and flexible application of strategies across situations may be particularly beneficial for caregivers managing difficult or variable emotions in themselves and their child.
Quantitative Analysis of Tennis Players' Momentum and Influencing Factors Based on the XGBoost Model
Authors: WANG Donghong , ZHANG Xu , JIANG Wenli 
Published: June 2024

Abstract

Momentum can directly or indirectly affect a tennis player's mentality and the trajectory of the game, thereby changing the outcome of the match. The article provides a clear quantitative description of the concept of momentum in tennis, analyzing the impact of momentum fluctuations on the trajectory of the game and the athlete's scoring; a secondary indicator system is established, and the weights of each indicator are determined through expert analysis method, CRITIC weighting method, and hierarchical analysis method. The final value of momentum is randomly tested with a random walk model. The results show that momentum is not random but influenced by specific factors, indicating that there is a certain correlation between the fluctuation of momentum and the success of the player. Due to the non-normal distribution of momentum, using the XGBoost model and Shap feature importance analysis can determine the significant influence of factors such as the distance run by athletes and the speed at which they hit the tennis ball. Moreover, the model demonstrated excellent performance in five randomly selected matches, with low error indicators and high fitting degrees,and an R² coefficient of up to 0.9814, showing the model's high precision and strong generalization capability.
16S rRNA sequencing and Targeted Metabolomics Analyses Reveal Rhizosphere Microbial Diversity and Flavonoids Dynamics in Scutellaria baicalensis Georgi
Authors: Wang Yan , Yan Feng , Guo Quan , Tang Jing , Hu Xiaochen , Chen Ying , Yan Yonggang , Gao Jing , Zhang Gang , Li Yimin 
Published: June 2024

Abstract

Aims
Scutellaria baicalensis Georgi is a commonly used bulk Chinese medicinal material in China. Clinically, it is often divided into Ziqin and Kuqin respectively into medicine, and herbal research and pharmacological studies have shown that Ziqin and Kuqin have different effects. Therefore, we collected the HuangQin and its rhizosphere soils at different growth periods to explore the influence of soil microorganisms on flavonoid synthesis in HuangQin.
Methods
In this study, high-throughput sequencing and the UPLC-MS/MS-based metabolomics method were used to sequencing 16S rRNA sequencing and metabolic analysis of HuangQin rhizosphere soil samples and HuangQin, respectively.
Results
The results showed that the number of OTUs for the four years were 7594, 10227, 10280, and 9796, respectively. And with prolonged cultivation, the abundance of Pseudarthrobacter, Achromobacter and other beneficial bacteria gradually decreased. A total of 552 secondary metabolites were identified in the metabolome. Correlation analysis of soil physicochemical properties and differential microorganisms showed that Pseudarthrobacter was significantly correlated with TN, AN, AK, and NH4+-N, Nocardioides exhibited a notable correlation with TN and AK, as well as a significant association with AN and pH, and there is a significant correlation between Haliangium and AP.
Conclusion
This study showed that soil physicochemical properties and soil microorganisms would affect the accumulation of flavonoids in S. baicalensis, which provided an important basis for improving the quality of S. baicalensis through reasonable fertilization and precise microbiota management.