bluuebunny/crossref_metadata_2025_split huggingface dataset:
The antimicrobial propeptide hCAP-18 plasma levels in neutropenia of various aetiologies: a prospective study
Authors: Ye Ying , Carlsson Göran , Karlsson-Sjöberg Jenny M. T. , Borregaard Niels , Modéer Thomas U. , Andersson Mats L. , Pütsep Katrin L-A. 
Published: June 2015

AbstractThe underlying cause of neutropenia may be difficult to determine due to similar clinical presentation in many neutropenic conditions. The neutrophil protein hCAP-18 (pro-LL-37) is a major component of neutrophil secondary granules and in this prospective study we assessed the use of hCAP-18 levels in blood plasma for differential diagnosis of neutropenic patients (n = 133) of various aetiologies. Plasma levels of hCAP-18 were determined using immunoblot and ELISA. Patients with severe congenital neutropenia (n = 23) presented with the lowest levels of plasma hCAP-18 and differential diagnostic accuracy revealed high sensitivity (100%) and specificity (98.8%) for hCAP-18 ELISA. The correlation coefficient of the hCAP-18 ELISA versus immunoblotting was (R = 0.831) and that of the peptide LL-37 ELISA versus immunoblotting was (R = 0.405) (P < 0.001). Plasma hCAP-18 levels thus displayed high diagnostic value in differential diagnosis of chronic neutropenia. Neutropenic patients with Shwachman-Diamond syndrome, Barth syndrome, Cohen syndrome, acute myeloid leukaemia and specific granule deficiency presented with reduced plasma hCAP-18 levels as well. The blood plasma level of hCAP-18 was thus low in conditions in which the neutrophil antibacterial propeptide hCAP-18 is deficient, i.e. severe congenital neutropenia and neutrophil-specific granule deficiency and in conditions in which bone marrow myelopoiesis is negatively affected.
Photothermal ablation of bone metastasis of breast cancer using PEGylated multi-walled carbon nanotubes
Authors: Lin Zhen , Liu Yi , Ma Xueming , Hu Shaoyu , Zhang Jiawei , Wu Qian , Ye Wenbin , Zhu Siyuan , Yang Dehong , Qu Dongbin , Jiang Jianming 
Published: June 2015

AbstractThis study investigates therapeutic efficacy of photothermal therapy (PTT) in an orthotropic xenograft model of bone metastasis of breast cancer. The near-infrared (NIR) irradiation on Multi-Walled Carbon Nanotubes (MWNTs) resulted in a rapid heat generation which increased with the MWNTs concentration up to 100 μg/ml. MWNTs alone exhibited no toxicity, but inclusion of MWNTs dramatically decreased cell viability when combined with laser irradiation. Thermographic observation revealed that treatment with 10 μg MWNTs followed by NIR laser irradiation resulted in a rapid increase in temperature up to 73.4±11.98 °C in an intraosseous model of bone metastasis of breast cancer. In addition, MWNTs plus NIR laser irradiation caused a remarkably greater suppression of tumor growth compared with treatment with either MWNTs injection or NIR irradiation alone, significantly reducing the amount of tumor-induced bone destruction. All these demonstrate the efficacy of PTT with MWNTs for bone metastasis of breast cancer.
Whole genome amplification with SurePlex results in better copy number alteration detection using sequencing data compared to the MALBAC method
Authors: Deleye Lieselot , Coninck Dieter De , Christodoulou Christodoulos , Sante Tom , Dheedene Annelies , Heindryckx Björn , Abbeel Etienne Van den , Sutter Petra De , Menten Björn , Deforce Dieter , Nieuwerburgh Filip Van 
Published: June 2015

AbstractCurrent whole genome amplification (WGA) methods lead to amplification bias resulting in over- and under-represented regions in the genome. Nevertheless, certain WGA methods, such as SurePlex and subsequent arrayCGH analysis, make it possible to detect copy number alterations (CNAs) at a 10 Mb resolution. A more uniform WGA combined with massive parallel sequencing (MPS), however, could allow detection at higher resolution and lower cost. Recently, MALBAC, a new WGA method, claims unparalleled performance. Here, we compared the well-established SurePlex and MALBAC WGA for their ability to detect CNAs in MPS generated data and, in addition, compared PCR-free MPS library preparation with the standard enrichment PCR library preparation. Results showed that SurePlex amplification led to more uniformity across the genome, allowing for a better CNA detection with less false positives compared to MALBAC amplified samples. An even more uniform coverage was observed in samples following a PCR-free library preparation. In general, the combination of SurePlex and MPS led to the same chromosomal profile compared to a reference arrayCGH from unamplified genomic DNA, underlining the large potential of MPS techniques in CNA detection from a limited number of DNA material.
Identification of BST-2/tetherin-induced hepatitis B virus restriction and hepatocyte-specific BST-2 inactivation
Authors: Lv Mingyu , Zhang Biao , Shi Ying , Han Zhu , Zhang Yan , Zhou Yulai , Zhang Wenyan , Niu Junqi , Yu Xiao-Fang 
Published: June 2015

AbstractBST-2/tetherin is an interferon-inducible antiviral protein that blocks the release of various enveloped viruses, including HIV-1. Hepatitis B virus (HBV), a major cause of liver disease, belongs to the Hepadnaviridae family of enveloped DNA viruses. Whether BST-2 regulates HBV production is largely unknown. In this report, we have demonstrated that HBV particle release is modulated by BST-2 in a cell type-dependent fashion. In HEK293T cells, ectopically expressed or interferon-induced BST-2 strongly inhibited HBV release. BST-2 co-localized with HBV surface protein at multivesicular bodies (MVBs) and physically interacted with HBV particles. However, exogenous BST-2-induced HBV restriction was weak in Huh-7 hepatoma cells and the interferon-induced anti-HBV effect was independent of BST-2 induction in hepatic L02 cells. Notably, HBV could promote HIV-1 ΔVpu virus release from BST-2-positive HepG2 hepatoma cells but not HeLa cells, whereas Vpu failed to efficiently inhibit BST-2-induced HBV restriction. HBx exhibited an enhanced interaction and co-localization with BST-2 in hepatocytes. These observations indicate that BST-2 restricts HBV production at intracellular MVBs but is inactivated by HBV through a novel mechanism requiring hepatocyte-specific cellular co-factors or a hepatocyte-specific environment. Further understanding of BST-2-induced HBV restriction may provide new therapeutic targets for future HBV treatments.
Q‐switching and mode‐locking pulse generation with graphene oxide paper‐based saturable absorber
Authors: Harun Sulaiman Wadi , Sabran Muhamad Burhan Shah , Azooz Salam Mahdi , Zulkifli Ahmad Zarif , Ismail Mohd Afiq , Ahmad Harith 
Published: June 2015

Q‐switched and mode‐locked erbium‐doped fibre lasers (EDFLs) are demonstrated by using non‐conductive graphene oxide (GO) paper as a saturable absorber (SA). A stable and self‐starting Q‐switched operation was achieved at 1534.4 nm by using a 0.8 m long erbium‐doped fibre (EDF) as a gain medium. The pulse repetition rate changed from 14.3 to 31.5 kHz, whereas the corresponding pulse width decreased from 32.8 to 13.8 µs as the pump power increased from 22 to 50.5 mW. A narrow spacing dual‐wavelength Q‐switched EDFL could also be realised by including a photonics crystal fibre and a tunable Bragg filter in the setup. It can operate at a maximum repetition rate of 31 kHz, with a pulse duration of 7.04 µs and pulse energy of 2.8 nJ. Another GOSA was used to realise mode‐locked EDFL in a different cavity consisting of a 1.6 m long EDF in conjunction with 1480 nm pumping. The laser generated a soliton pulse train with a repetition rate of 15.62 MHz and pulse width of 870 fs. It is observed that the proposed fibre lasers have a low pulsing threshold pump power as well as a low damage threshold.
Fault rheology beyond frictional melting
Authors: Lavallée Yan , Hirose Takehiro , Kendrick Jackie E. , Hess Kai-Uwe , Dingwell Donald B. 
Published: June 2015

Significance
During an earthquake, the mechanical work of friction along the fault is partly dissipated as heat, which results in rock melting and pseudotachylyte generation along the fault plane. Beyond this point, it is generally believed that Newtonian viscous forces regulate slip. Molten rocks are, however, viscoelastic bodies exhibiting the so-called glass transition of liquid-like to solid-like response to stresses that allows the melt itself to fracture. This simple fact, the significance of which is demonstrated in high-speed frictional melting experiments, redefines fault slip rheology in major earthquakes.
Virtual groundwater transfers from overexploited aquifers in the United States
Authors: Marston Landon , Konar Megan , Cai Ximing , Troy Tara J. 
Published: June 2015

Significance
Irrigated agriculture is contributing to the depletion of the Central Valley, High Plains, and Mississippi Embayment aquifer systems. Agricultural production within these aquifer regions comprises a significant portion of the domestic and international cereal supply; thus, potential food security implications arise if production significantly decreases to bring groundwater withdrawals within sustainable limits. For the first time to our knowledge, this study tracks and quantifies the food and embodied groundwater resources from these aquifer systems to their final destination and determines the major US cities, US states, and countries that are currently most reliant upon them. Tracing virtual groundwater transfers highlights the role of distant demands on local groundwater sustainability and the fact that aquifer depletion must be considered within its global context.
PI3K therapy reprograms mitochondrial trafficking to fuel tumor cell invasion
Authors: Caino M. Cecilia , Ghosh Jagadish C. , Chae Young Chan , Vaira Valentina , Rivadeneira Dayana B. , Faversani Alice , Rampini Paolo , Kossenkov Andrew V. , Aird Katherine M. , Zhang Rugang , Webster Marie R. , Weeraratna Ashani T. , Bosari Silvano , Languino Lucia R. , Altieri Dario C. 
Published: June 2015

Significance
Despite the promise of personalized cancer medicine, most molecular therapies produce only modest and short-lived patient gains. In addition to drug resistance, it is also possible that tumors adaptively reprogram their signaling pathways to evade therapy-induced “stress” and, in the process, acquire more aggressive disease traits. We show here that small-molecule inhibitors of PI3K, a cancer node and important therapeutic target, induce transcriptional and signaling reprogramming in tumors. This involves the trafficking of energetically active mitochondria to subcellular sites of cell motility, where they provide a potent, “regional” energy source to support tumor cell invasion. Although this response may paradoxically increase the risk of metastasis during PI3K therapy, targeting mitochondrial reprogramming is feasible, and could provide a novel therapeutic strategy.
Highly compacted biodegradable DNA nanoparticles capable of overcoming the mucus barrier for inhaled lung gene therapy
Authors: Mastorakos Panagiotis , da Silva Adriana L. , Chisholm Jane , Song Eric , Choi Won Kyu , Boyle Michael P. , Morales Marcelo M. , Hanes Justin , Suk Jung Soo 
Published: June 2015

SignificanceTherapeutically relevant lung gene therapy is yet to be achieved. We introduce a highly translatable gene delivery platform for inhaled gene therapy based on state-of-the-art biodegradable polymers, poly(β-amino esters). The newly designed system is capable of overcoming challenging biological barriers, thereby providing robust transgene expression throughout the entire luminal surface of mouse lungs. Moreover, it provides markedly greater overall transgene expression in vivo compared with gold standard platforms, including a clinically tested system. The clinical relevance is further underscored by the excellent safety profile as well as long-term and consistent transgene expression achieved following a single and repeated administrations, respectively.
Origin and dynamics of admixture in Brazilians and its effect on the pattern of deleterious mutations
Authors: Kehdy Fernanda S. G. , Gouveia Mateus H. , Machado Moara , Magalhães Wagner C. S. , Horimoto Andrea R. , Horta Bernardo L. , Moreira Rennan G. , Leal Thiago P. , Scliar Marilia O. , Soares-Souza Giordano B. , Rodrigues-Soares Fernanda , Araújo Gilderlanio S. , Zamudio Roxana , Sant Anna Hanaisa P. , Santos Hadassa C. , Duarte Nubia E. , Fiaccone Rosemeire L. , Figueiredo Camila A. , Silva Thiago M. , Costa Gustavo N. O. , Beleza Sandra , Berg Douglas E. , Cabrera Lilia , Debortoli Guilherme , Duarte Denise , Ghirotto Silvia , Gilman Robert H. , Gonçalves Vanessa F. , Marrero Andrea R. , Muniz Yara C. , Weissensteiner Hansi , Yeager Meredith , Rodrigues Laura C. , Barreto Mauricio L. , Lima-Costa M. Fernanda , Pereira Alexandre C. , Rodrigues Maíra R. , Tarazona-Santos Eduardo ,   The Brazilian EPIGEN Project Consortium, Alcantara-Neves Neuza , Araújo Nathalia M. , Carvalho Márcio L.B. , Conceição Jackson Santos , Firmo Josélia O.A. , Gigante Denise P. , Meira Lindolfo , Muniz-Queiroz Thais , Oliveira Guilherme C. , Oliveira Isabel O. , Peixoto Sérgio V. , Proietti Fernando A. , Rodrigues Domingos C. , Santolalla Meddly L. , Strina Agostino , Zolini Camila 
Published: June 2015

Significance
The EPIGEN Brazil Project is the largest Latin-American initiative to study the genomic diversity of admixed populations and its effect on phenotypes. We studied 6,487 Brazilians from three population-based cohorts with different geographic and demographic backgrounds. We identified ancestry components of these populations at a previously unmatched geographic resolution. We broadened our understanding of the African diaspora, the principal destination of which was Brazil, by revealing an African ancestry component that likely derives from the slave trade from Bantu/eastern African populations. In the context of the current debate about how the pattern of deleterious mutations varies between Africans and Europeans, we use whole-genome data to show that continental admixture is the main and complex determinant of the amount of deleterious genotypes in admixed individuals.
Small GTP-binding protein Ran is regulated by posttranslational lysine acetylation
Authors: de Boor Susanne , Knyphausen Philipp , Kuhlmann Nora , Wroblowski Sarah , Brenig Julian , Scislowski Lukas , Baldus Linda , Nolte Hendrik , Krüger Marcus , Lammers Michael 
Published: June 2015

Significance
The small GTPase Ran plays fundamental roles in cellular processes such as nucleo-cytoplasmic transport, mitotic spindle formation, and nuclear envelope assembly. Recently, Ran was found to be lysine acetylated, among others, in functionally important regions such as switch I and switch II. Using the genetic code expansion concept we show that lysine acetylation affects many important aspects of Ran function such as RCC1-catalyzed nucleotide exchange, intrinsic nucleotide hydrolysis, import/export complex formation, and Ran subcellular localization. Finally, we present evidence for a regulation of Ran acetylation by sirtuin deacetylases and lysine acetyltransferases.
CD300f associates with IL-4 receptor α and amplifies IL-4–induced immune cell responses
Authors: Moshkovits Itay , Karo-Atar Danielle , Itan Michal , Reichman Hadar , Rozenberg Perri , Morgenstern-Ben-Baruch Netali , Shik Dana , Ejarque-Ortiz Aroa , Hershko Alon Y. , Tian Linjie , Coligan John E. , Sayós Joan , Munitz Ariel 
Published: June 2015

SignificanceIL-4 receptor (R) α is a critical component in IL-4– and IL-13–mediated signaling and subsequent effector functions such as those observed in allergy. Thus, it is a primary therapeutic target in diseases such as atopic dermatitis and asthma. Despite extensive studies, it is unknown whether an additional receptor system exists that may act to amplify IL-4Rα signaling and subsequent IL-4/IL-13–induced responses. We now report that CD300f is physically associated with IL-4Rα and potently amplifies IL-4Rα–induced responses in vitro and in vivo. Our results establish CD300f as a previously unidentified IL-4Rα coreceptor. To the best of our knowledge, this is the first report of an additional receptor that serves to amplify the IL-4 signaling pathway.
Interventional Radiology Cases
Authors: Covey Anne M. , Pua Bradley P. , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
In 104 cases featuring over 500, high-quality images, Interventional Radiology Cases is a thorough and accessible review of the interventional procedures that radiology residents are expected to be familiar with upon completion of residency and general radiologists need to know for recertification examinations. The cases present both benign and malignant conditions and all pertinent imaging modalities incorporated including: CT, MR, PET, fluoroscopy, and ultrasound. Part of the Cases in Radiology series, this online resource follows the easy-to-use format of question and answer in which the patient history is provided first, and radiologic findings, differential diagnosis, teaching points, next steps in management, and suggestions for furthering reading are revealed below.
Case 8
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Biopsy section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 11
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Biopsy section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 23
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Ablation section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 53
Authors: Covey Anne M. , Pua Bradley B. , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Gastrointestinal/Biliary Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 54
Authors: Covey Anne M. , Pua Bradley B. , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Gastrointestinal/Biliary Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 60
Authors: Covey Anne M. , Pua Bradley B. , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Gastrointestinal/Biliary Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 69
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Venous Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 72
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Venous Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 78
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Arterial Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 84
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Arterial Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 85
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Arterial Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Case 88
Authors: Covey Anne M. , Pua Bradley B , Aguado Allison , Madoff David C. 
Published: June 2015

Abstract
This is a case from the Arterial Intervention section of Interventional Radiology Cases. The specific content of each case and its diagnosis are necessarily hidden from this abstract. Each case contains case history, followed immediately by the diagnosis, which is supported with findings, differential diagnosis, teaching points, management, and further reading suggestions.
Cardiopulmonary resuscitation for the twenty-first century
Authors: Galloway Robert , Chamberlain Douglas 
Published: June 2015

Abstract
The chapter provides a realistic and relevant overview of the challenges facing the emergency physician dealing with cardiac arrest. It highlights the changing emphases now placed both in the pre-hospital and immediate resuscitation room settings and outlines the most recent key changes to the ALS algorithm.
It examines the evidence base for three clinical questions discussing the need for high-quality chest compressions and the mechanical methods in which these can be delivered. It discusses the role of therapeutic hypothermia in all cases of cardiac arrest and finally the strategies for maximizing care after the return of spontaneous circulation as well as discussing the leadership skills required and certain practical techniques such as bedside echocardiography.