bluuebunny/crossref_metadata_2025_split huggingface dataset:
Translated and culturally adapted internet-delivered cognitive therapy for social anxiety disorder in Japanese clinical settings: study protocol for a randomised controlled trial
Authors: Yoshinaga Naoki , Thew Graham R. , Hayashi Yuta , Tanoue Hiroki , Nakai Michikazu , Clark David M. 
Published: July 2024

Abstract
Background
Cognitive therapy for social anxiety disorder (CT-SAD) has extensive empirical support and is recommended in several national guidelines. However, ensuring access to evidence-based psychological therapies such as CT-SAD remains a global challenge. An internet-delivered version of this treatment protocol (iCT-SAD) has recently been developed in the UK as a way to overcome this challenge, demonstrating comparable outcomes to face-to-face CT-SAD whilst requiring less therapist time per client. Initial findings also suggest its cross-cultural transferability, but the previous studies in other cultural settings used the English language programme and only included English-fluent participants as a second language. It is not yet known what outcomes can be achieved once the programme has been translated and adapted for a different cultural context. Therefore, this trial aims to evaluate the clinical efficacy of Japanese iCT-SAD when combined with treatment as usual (TAU) in clients with SAD.

Methods
This two-arm, parallel-group, superiority randomised controlled trial will recruit 60 Japanese participants with SAD, randomly assigning them to either Japanese iCT-SAD + TAU or TAU alone at a ratio of 1:1. The primary outcome measure is the self-report Liebowitz Social Anxiety Scale, and secondary.outcomes include other measures of social anxiety symptoms and processes, general mood and functioning, and response to treatment. We will also assess treatment acceptability and gather participant feedback. Assessments will take place at baseline (week 0), mid-treatment (week 8), and post-treatment (week 15), with a further 3-month follow-up (week 27) for the iCT-SAD + TAU arm. The primary analyses will be conducted on an intent-to-treat basis, comparing the primary and secondary outcome measures between groups using linear mixed-effect models, along with additional mediation analysis.

Discussion
Investigating the efficacy of translated and culturally adapted iCT-SAD in different cultural contexts is an important step in evaluating the global reach of internet interventions. This trial will provide valuable insights into the effects of iCT-SAD combined with usual care, and how this treatment could be delivered in routine clinical settings in Japan.

Trial registration
International Standard Randomized Controlled Trials (ISRCTN), ISRCTN82859645, registered on January 19, 2024. UMIN Clinical Trials Registry (UMIN-CTR), UMIN000052702, registered on November 6, 2023.
Exploring sources of inaccuracy and irreproducibility in the CDC bottle bioassay through direct insecticide quantification
Authors: Peard Evah F. , Luu Calvin , Hageman Kimberly J. , Sepesy Rose , Bernhardt Scott A. 
Published: July 2024

Abstract
Background
The Centers for Disease Control and Prevention (CDC) bottle bioassay is a commonly used susceptibility test for measuring insect response to insecticide exposure. However, inconsistencies and high variability in insect response when conducting CDC bottle bioassays have been reported in previous publications. We hypothesized that the CDC bottle bioassay results may be compromised when expected and actual insecticide concentrations in the bottles are not equivalent and that inadequate bottle cleaning and/or loss during insecticide introduction and bottle storage steps could be responsible. We explored this hypothesis by quantifying insecticides using gas chromatography tandem mass spectrometry (GC-MS/MS) in bottles that had been cleaned, prepared, and stored according to the CDC guidelines.

Methods
We investigated the bottle cleaning, preparation, and storage methods outlined in the CDC bottle bioassay procedure to identify sources of irreproducibility. We also investigated the effectiveness of cleaning bottles by autoclaving because this method is commonly used in insecticide assessment laboratories. The two insecticides used in this study were chlorpyrifos and lambda-cyhalothrin (λ-cyhalothrin). Insecticides were removed from glass bioassay bottles by rinsing with ethyl-acetate and n-hexane and then quantified using GC-MS/MS.

Results
The CDC bottle bioassay cleaning methods did not sufficiently remove both insecticides from the glass bottles. The cleaning methods removed chlorpyrifos, which has higher water solubility, more effectively than λ-cyhalothrin. Chlorpyrifos experienced significant loss during the bottle-coating process whereas λ-cyhalothrin did not. As for bottle storage, no significant decreases in insecticide concentrations were observed for 6 h following the initial drying period for either insecticide.

Conclusions
The CDC bottle bioassay protocol is susceptible to producing inaccurate results since its recommended bottle cleaning method is not sufficient and semi-volatile insecticides can volatilize from the bottle during the coating process. This can lead to the CDC bottle bioassay producing erroneous LC50 values. High levels of random variation were also observed in our experiments, as others have previously reported. We have outlined several steps that CDC bottle bioassay users could consider that would lead to improved accuracy and reproducibility when acquiring toxicity data.

Graphical Abstract
Dengue rhabdomyolysis successfully treated with hemoperfusion using CytoSorb® in combination with continuous renal replacement therapy: a case report
Authors: Samarasingha Piyum , Karunatilake Harindra , Jayanaga Ananda , Jayawardhana Hansani , Priyankara Dilshan 
Published: July 2024

Abstract
Background
Dengue fever is a mosquito-borne viral infection with a broad spectrum of clinical manifestations. Expanded dengue syndrome includes unusual manifestations that do not fall into the categories of dengue fever, dengue hemorrhagic fever, or dengue shock syndrome. Rhabdomyolysis causing acute renal failure in dengue is one such unusual manifestation, the pathophysiology of which is incompletely understood.

Case presentation
We describe a 21-year-old Sri Lankan man with dengue fever who developed severe rhabdomyolysis and acute kidney injury with extremely high creatinine phosphokinase levels (> 2 million U/L). Management of this patient was challenging as his creatinine phosphokinase kept rising with persistent anuria despite hydration, intermittent hemodialysis, and, later, continuous venovenous hemodiafiltration. Further therapeutic options were explored, and CytoSorb® adsorber was added as an adjunct to continuous venovenous hemodiafiltration, following which we observed a marked reduction in his creatinine phosphokinase and myoglobin levels over the next 12 hours and complete renal recovery over the next 5 weeks.

Conclusion
We report a rare case of significant rhabdomyolysis secondary to dengue infection leading to acute kidney injury. Continuous venovenous hemodiafiltration performed with the hemofilter Pecopen 140 was ineffective, and the addition of CytoSorb® adsorber as an adjunct therapy to continuous venovenous hemodiafiltration may have a potential benefit in removing high-molecular-weight proteins such as myoglobin.
Genome-scale CRISPR-Cas9 screening in stem cells: theories, applications and challenges
Authors: Zhou Heng , Ye Peng , Xiong Wei , Duan Xingxiang , Jing Shuili , He Yan , Zeng Zhi , Wei Yen , Ye Qingsong 
Published: July 2024

AbstractDue to the rapid development of stem cell technology, there have been tremendous advances in molecular biological and pathological research, cell therapy as well as organoid technologies over the past decades. Advances in genome editing technology, particularly the discovery of clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-related protein 9 (Cas9), have further facilitated the rapid development of stem cell researches. The CRISPR-Cas9 technology now goes beyond creating single gene editing to enable the inhibition or activation of endogenous gene loci by fusing inhibitory (CRISPRi) or activating (CRISPRa) domains with deactivated Cas9 proteins (dCas9). These tools have been utilized in genome-scale CRISPRi/a screen to recognize hereditary modifiers that are synergistic or opposing to malady mutations in an orderly and fair manner, thereby identifying illness mechanisms and discovering novel restorative targets to accelerate medicinal discovery investigation. However, the application of this technique is still relatively rare in stem cell research. There are numerous specialized challenges in applying large-scale useful genomics approaches to differentiated stem cell populations. Here, we present the first comprehensive review on CRISPR-based functional genomics screening in the field of stem cells, as well as practical considerations implemented in a range of scenarios, and exploration of the insights of CRISPR-based screen into cell fates, disease mechanisms and cell treatments in stem cell models. This review will broadly benefit scientists, engineers and medical practitioners in the areas of stem cell research.
The whole is greater than the sum of its parts: improving music source separation by bridging networks
Authors: Sawata Ryosuke , Takahashi Naoya , Uhlich Stefan , Takahashi Shusuke , Mitsufuji Yuki 
Published: July 2024

AbstractThis paper presents the crossing scheme (X-scheme) for improving the performance of deep neural network (DNN)-based music source separation (MSS) with almost no increasing calculation cost. It consists of three components: (i) multi-domain loss (MDL), (ii) bridging operation, which couples the individual instrument networks, and (iii) combination loss (CL). MDL enables the taking advantage of the frequency- and time-domain representations of audio signals. We modify the target network, i.e., the network architecture of the original DNN-based MSS, by adding bridging paths for each output instrument to share their information. MDL is then applied to the combinations of the output sources as well as each independent source; hence, we called it CL. MDL and CL can easily be applied to many DNN-based separation methods as they are merely loss functions that are only used during training and do not affect the inference step. Bridging operation does not increase the number of learnable parameters in the network. Experimental results showed that the validity of Open-Unmix (UMX), densely connected dilated DenseNet (D3Net) and convolutional time-domain audio separation network (Conv-TasNet) extended with our X-scheme, respectively called X-UMX, X-D3Net and X-Conv-TasNet, by comparing them with their original versions. We also verified the effectiveness of X-scheme in a large-scale data regime, showing its generality with respect to data size. X-UMX Large (X-UMXL), which was trained on large-scale internal data and used in our experiments, is newly available at https://github.com/asteroid-team/asteroid/tree/master/egs/musdb18/X-UMX.
Spatial distribution and pedigree age of intangible cultural heritage along the Grand Canal of China
Authors: Zhang Shuying , Zhang Xin , Liu Jiaming 
Published: July 2024

Abstract
Intangible cultural heritage is the carrier of national memory and a historical witness. Exploring the spatial distribution and chronological evolution of intangible cultural heritage is of great significance to understand the cultural integrity and historical continuity. Taking the intangible cultural heritage along the Grand Canal as an example, this study carries out spatial deconstruction based on the extent of the spread of intangible cultural heritage and reveales the aggregation pattern through spatial autocorrelation analysis. Then, the historical process and the spatial center of gravity are shown through an evolution tree model. The influencing factors are illustrated via the methods of Geodetector and qualitative analysis. The results are as follows: (1) The two ends of the canal exhibit obvious advantages in the number of intangible cultural heritage items; however, the existence of a spatial mismatch at the city–county level makes the southern endpoint occupy a dominant position. (2) Although there is a high–high aggregation pattern along the whole canal, 29.82% of the counties are distributed widely with low–low aggregation. (3) The spatial center of intangible cultural heritage items from different historical periods generally presents a changing path from north to south and returning north. (4) Intangible cultural heritage items are mainly distributed in areas with a prosperous culture, a high level of economic development, and strong policy and financial support from government. Changes in the natural environment, population migration, economic development, war disasters, and canal shipping have profound impacts over time. This study provides a new idea for the methodological advancement of spatial distribution of intangible cultural heritage, as well as studies on local cultural identity and regional consistency.
Diverse bacterial consortia: key drivers of rhizosoil fertility modulating microbiome functions, plant physiology, nutrition, and soybean grain yield
Authors: Moretti Luiz Gustavo , Crusciol Carlos Alexandre Costa , Leite Marcio Fernandes Alves , Momesso Letusa , Bossolani João William , Costa Ohana Yonara Assis , Hungria Mariangela , Kuramae Eiko Eurya 
Published: July 2024

AbstractSoybean cultivation in tropical regions relies on symbioses with nitrogen-fixing Bradyrhizobium and plant growth-promoting bacteria (PGPBs), reducing environmental impacts of N fertilizers and pesticides. We evaluate the effects of soybean inoculation with different bacterial consortia combined with PGPBs or microbial secondary metabolites (MSMs) on rhizosoil chemistry, plant physiology, plant nutrition, grain yield, and rhizosphere microbial functions under field conditions over three growing seasons with four treatments: standard inoculation of Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens consortium (SI); SI plus foliar spraying with Bacillus subtilis (SI + Bs); SI plus foliar spraying with Azospirillum brasilense (SI + Az); and SI plus seed application of MSMs enriched in lipo-chitooligosaccharides extracted from B. diazoefficiens and Rhizobium tropici (SI + MSM). Rhizosphere microbial composition, diversity, and function was assessed by metagenomics. The relationships between rhizosoil chemistry, plant nutrition, grain yield, and the abundance of microbial taxa and functions were determined by generalized joint attribute modeling. The bacterial consortia had the most significant impact on rhizosphere soil fertility, which in turn affected the bacterial community, plant physiology, nutrient availability, and production. Cluster analysis identified microbial groups and functions correlated with shifts in rhizosoil chemistry and plant nutrition. Bacterial consortia positively modulated specific genera and functional pathways involved in biosynthesis of plant secondary metabolites, amino acids, lipopolysaccharides, photosynthesis, bacterial secretion systems, and sulfur metabolism. The effects of the bacterial consortia on the soybean holobiont, particularly the rhizomicrobiome and rhizosoil fertility, highlight the importance of selecting appropriate consortia for desired outcomes. These findings have implications for microbial-based agricultural practices that enhance crop productivity, quality, and sustainability.
Graphical Abstract
Pathological mechanisms and novel drug targets in fibrotic interstitial lung disease
Authors: Nishioka Yasuhiko , Araya Jun , Tanaka Yoshiya , Kumanogoh Atsushi 
Published: July 2024

Abstract
Background
Interstitial lung diseases (ILDs) are a diverse group of conditions characterized by inflammation and fibrosis in the lung. In some patients with ILD, a progressive fibrotic phenotype develops, which is associated with an irreversible decline in lung function and a poor prognosis.

Main body
The pathological mechanisms that underlie this process culminate in fibroblast activation, proliferation, and differentiation into myofibroblasts, which deposit extracellular matrix proteins and result in fibrosis. Upstream of fibroblast activation, epithelial cell injury and immune activation are known initiators of fibrosis progression, with multiple diverse cell types involved. Recent years have seen an increase in our understanding of the complex and interrelated processes that drive fibrosis progression in ILD, in part due to the advent of single-cell RNA sequencing technology and integrative multiomics analyses. Novel pathological mechanisms have been identified, which represent new targets for drugs currently in clinical development. These include phosphodiesterase 4 inhibitors and other molecules that act on intracellular cyclic adenosine monophosphate signaling, as well as inhibitors of the autotaxin-lysophosphatidic acid axis and $$\alpha_v$$

α
v

integrins. Here, we review current knowledge and recent developments regarding the pathological mechanisms that underlie progressive fibrotic ILD, including potential therapeutic targets.

Conclusion
Knowledge of the pathological mechanisms that drive progressive fibrosis in patients with ILD has expanded, with the role of alveolar endothelial cells, the immune system, and fibroblasts better elucidated. Drugs that target novel mechanisms hold promise for expanding the future therapeutic armamentarium for progressive fibrotic ILD.
Readmission rate among survived patients with acute respiratory failure: 1-year study
Authors: Elsayed Tamer Awad , Farrag Nesrine Saad , Abdelgawad Taha Taha 
Published: July 2024

Abstract
Context
Critically ill patients, especially those with respiratory failure associated with multiple comorbidities, are at risk of recurrent ICU admission and consuming a significant portion of medical resources.

Aim
To study the risk factors and common etiologies of readmission among surviving patients with acute respiratory failure in Mansoura University’s respiratory intensive care unit during the year 2023.

Settings and design
This was a retrospective cohort study.

Patients and methods
This study included all patients with acute respiratory failure who were admitted to Mansoura University’s respiratory intensive care unit (RICU), from January 2023 to December 2023. Deceased patients after initial admission or those with incomplete data were not included in the study. All data related to patient’s demographics, type of respiratory failure, type of respiratory support, associated comorbidities, length of ICU stay, and causes of readmission were collected from the registration system database.

Results
Eight-hundred-thirty (830) cases that were admitted to the Mansoura University’s RICU with respiratory failure in the year 2023 were enrolled in the study, and 84 cases (10.1%) of them were readmitted. COPD exacerbation was the most common cause of readmission (35.7%) followed by pneumonia (21.4%) and OSA exacerbation (20.2%). Results showed that the significant independent predictors of readmission were being male [AOR (95% CI): 1.8 (1.01–3.1), p: 0.046], having organ failure (renal/liver) [AOR (95% CI): 29.9 (7.9–113.4), p ≤ 0.001], and length of ICU stay more than 12 days [AOR (95% CI): 4.8 (2.6–8.6), p ≤ 0.001]. Also, the type of respiratory failure and the type of respiratory support received were significantly associated with readmission in the univariate analysis; however, they were insignificant in the multivariate analysis.

Conclusion
The rate of readmission was not high (only 10.3%). The most common causes of readmission were COPD exacerbation, followed by pneumonia and OSA exacerbation, respectively. Type of respiratory failure, male gender, the presence of other organ failure, and length of ICU stay are significant independent predictors of readmission in Mansoura University’s RICU during the year 2023. Therefore, higher-risk individuals should receive attention and careful assessment before discharge from the ICU to reduce the rate of readmission.

Trial registration
ClinicalTrials.gov identifier: NCT06291636.
Subcutaneous vs Intravenous Trastuzumab/Pertuzumab: A Time and Motion Substudy of a Phase II Trial of Adjuvant Trastuzumab/Pertuzumab for Stage I HER2+ Breast Cancer (ADEPT trial)
Authors: Waks Adrienne G. , Chen Emily L. , Graham Noah , Frey Anna Mae , Almeida Kenneth , Attaya Victoria , Ryding Cari , Abbass Ibrahim , Fung Anita , Sussell Jesse , Cortazar Patricia , Harvey Caroline , Leth Denise , Faggen Meredith , Sinclair Natalie , Walsh Jeanna , Tung Nadine , Sinclair Sarah , Lo Steve , Yardley Denise , Valero Vicente , Meisel Jane , Ballinger Tarah J. , Adams Sylvia , Carey Lisa A. , Rauch Julia K. , Abramson Vandana G. , Williams Nicole O. , Chen Wendy Y. , Leone Jose P. , Schumer Susan T. , Tayob Nabihah , Tolaney Sara M. 
Published: July 2024

PURPOSE The time required for in-clinic drug administration can substantially affect breast cancer patients' quality of life. Subcutaneous (SC) drug administration, as opposed to intravenous (IV), may reduce this time commitment. This study sought to estimate the difference in time burden between IV and SC administration of trastuzumab and pertuzumab (HP). METHODS We prospectively enrolled a subcohort of patients participating in the ADEPT trial (ClinicalTrials.gov identifier: NCT04569747 , investigating adjuvant HP plus endocrine therapy for stage I human epidermal growth factor receptor 2–positive breast cancer) to this single-arm crossover time and motion substudy. Patients received two cycles of IV HP followed by two cycles of SC HP. During each cycle, time points in drug preparation and administration were captured. The primary end point was total patient time in the treatment chair. Additional end points included total patient treatment experience time and total pharmacy workflow time. A sample size of 22 patients was estimated to provide 90.7% power with two-sided alpha .05 to detect a difference of 70 minutes in the primary end point by treatment arm (IV v SC). RESULTS Twenty-two patients were enrolled. The mean total patient time in the treatment chair was 61.8 minutes shorter with SC versus IV HP (22.5 v 84.3 minutes; P < .0001). The mean total patient treatment experience time (incorporating time spent waiting for treatment initiation and time spent in the treatment chair) was 81.8 minutes shorter for SC administration (96 v 177.8 minutes; P < .0001). The pharmacy workflow time was 78.2 minutes shorter for SC versus IV formulation (41 v 119.2 minutes; P < .0001). CONCLUSION SC administration of HP shortened patient time burden by approximately 1 hour. SC drug administration can facilitate faster workflows for health care professionals and improve patients' breast cancer treatment experience.