bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein nanomachines assembly modes: cell‐free expression and biochip perspectives
Authors: Daube Shirley S. , Bar‐Ziv Roy H. 
Published: July 2013

Large macromolecular assemblies are widespread in all cell types with diverse structural and functional roles. Whether localized to membranes, nuclei, or cytoplasm, multimeric protein–nucleic acid complexes may be viewed as sophisticated nanomachines, an inspiration to chemical design. The formation of large biological assemblies follows a complex and hierarchical self‐assembly process via ordered molecular recognition events. Serving a paradigm for biological assembly, extensive past studies of T4 bacteriophage and bacterial ribosomes by many groups have been revealing distinct design strategies, yet these two very different multimeric complexes share common mechanistic motifs. An emerging biochip approach highlights two conceptual notions to promote the study of assembly pathways: cell‐free expression provides coupling between synthesis and assembly; surface anchoring allows high‐resolution imaging of structural intermediates and opens up opportunities for rewiring a network by defining unnatural scaffolds for synthetic design applications. WIREs Nanomed Nanobiotechnol 2013, 5:613–628. doi: 10.1002/wnan.1227This article is categorized under:

Nanotechnology Approaches to Biology > Nanoscale Systems in Biology
Aspirin Use is Associated with Lower Prostate Cancer Risk in Male Carriers of BRCA Mutations
Authors: Cossack Matthew , Ghaffary Cameron , Watson Patrice , Snyder Carrie , Lynch Henry 
Published: July 2013

AbstractPrevious studies have shown that male BRCA mutation carriers stand at increased risk of developing prostate cancer and have concerns about developing cancer. Genetic counseling practitioners often discuss strategies for reducing the risk of cancer for patients at high risk due to their genetic background. Addressing modifiable health habits is one such strategy. Unfortunately, modifiable risk factors for prostate cancer have only been documented in the general population and have not yet been studied in the BRCA carrier subpopulation. Therefore, this study aimed to identify modifiable risk factors for prostate cancer in BRCA carriers. We examined prostate cancer risk factors in 74 men who were part of families with a BRCA mutation. This study examined nine dichotomous variables including: exercise, history of vasectomy, smoking history, alcohol use, finasteride use, statin use, aspirin use, coffee use, and vitamin use. The survey was sent to all cases of prostate cancer in the Hereditary Cancer Center Database at Creighton University with a known BRCA status. This study confirmed the protective benefits of daily aspirin use, which have been observed in previous studies of the general population, and suggests its benefit in BRCA carriers. Protective benefits from regular vigorous exercise and daily coffee use trended towards significance, but neither factor withstood the Bonferroni Correction for multiple comparisons.
Quantitative Analysis of Phytosterols in Edible Oils Using APCI Liquid Chromatography–Tandem Mass Spectrometry
Authors: Mo Shunyan , Dong Linlin , Hurst W. Jeffrey , van Breemen Richard B. 
Published: July 2013

AbstractPrevious methods for the quantitative analysis of phytosterols have usually used GC–MS and require elaborate sample preparation including chemical derivatization. Other common methods such as HPLC with absorbance detection do not provide information regarding the identity of the analytes. To address the need for an assay that utilizes mass selectivity while avoiding derivatization, a quantitative method based on LC–tandem mass spectrometry (LC–MS–MS) was developed and validated for the measurement of six abundant dietary phytosterols and structurally related triterpene alcohols including brassicasterol, campesterol, cycloartenol, β‐sitosterol, stigmasterol, and lupeol in edible oils. Samples were saponified, extracted with hexane and then analyzed using reversed phase HPLC with positive ion atmospheric pressure chemical ionization tandem mass spectrometry and selected reaction monitoring. The utility of the LC–MS–MS method was demonstrated by analyzing 14 edible oils. All six compounds were present in at least some of the edible oils. The most abundant phytosterol in all samples was β‐sitosterol, which was highest in corn oil at 4.35 ± 0.03 mg/g, followed by campesterol in canola oil at 1.84 ± 0.01 mg/g. The new LC–MS–MS method for the quantitative analysis of phytosterols provides a combination of speed, selectivity and sensitivity that exceed those of previous assays.
Relationship‐specific variability in adolescents' ethnic self‐labeling preferences
Authors: Kiang Lisa , Johnson Natasha C. 
Published: July 2013

ABSTRACTConsistency between adolescents' best‐fitting ethnic label and the labels uses in three different relationship contexts was compared and linked to adjustment among 154 9th‐10th graders (50% 9th; 56% female) of Asian descent. Results indicated that 57%, 61%, and 63% of adolescents reported best‐fitting labels that were inconsistent with the labels they would use with Asian, non‐Asian minority, and European American peers, respectively, and only 25% reported using the same label across all four situations. Inconsistency was not associated with gender or generation, but was linked with higher perceived discrimination. Despite its prevalence, there were few adjustment differences based on labeling inconsistency. One exception was that adolescents who reported inconsistency between best‐fitting labels and labels used with non‐Asian minority peers reported more negative emotions than those with concordant labels. Results and discussion highlight the need to continue investigating the contextual fluidity of ethnic labels, including predictors and developmental and cultural implications.
Experimental observation of backscattered light based on different coherence between incident laser beams
Authors: Meng Xiangfu , Wang Chen , An Honghai , Jia Guo , Zhou Huazhen , Fu Sizu 
Published: July 2013

AbstractRecent experimental results on NIF revealed a much higher stimulated Brillouin scattering (SBS) and stimulated Raman scattering (SRS) backscatter than expected; one possible reason was due to the coherence between incident laser beams. In our research, two laser beams (${\sim }1~\mathrm{ns} $, ${\sim }250~\mathrm{J} , 527~\mathrm{nm} $ in each one) with different coherent degrees between them from the SG-II facility were employed to irradiate an Au plate target; the backscatter of SBS and SRS in the range of the given solid angle had been measured. The results showed that it could change dramatically corresponding to the difference of the coherent degree between the two laser beams, and there was usually more intense backscatter the higher the coherent degree between the incident beams.
Spin-up of a two-component superfluid: analytic theory in arbitrary geometry
Authors: van Eysden Cornelis A. , Melatos A. 
Published: July 2013

AbstractThe impulsive spin-up of a two-component superfluid and its container is solved analytically for the first time in arbitrary geometry, generalizing the extensively studied case of single-fluid spin-up. The superfluid is modelled by the Hall–Vinen–Bekarevich–Khalatnikov (HVBK) two-fluid equations, neglecting non-hydrodynamic processes like vortex tension and pinning, which are weak in certain applications (e.g. neutron stars) and confined to boundary layers in the inviscid HVBK component in other applications (e.g. helium II spin-up in smooth-walled containers). Both components of the flow are found to be columnar. The spin-up time depends on the geometry, mutual friction coefficients, $B$, ${B}^{\prime } $, the Ekman number $E$, and the superfluid density fraction ${\rho }_{n} $. For $B\sim O(1)$, the inviscid component undergoes Ekman pumping due to strong coupling to the viscous component, and the azimuthal velocities are ‘locked together’ during the spin-up. For $B\lesssim {E}^{1/ 2} $, there is no Ekman pumping in the inviscid component and the inviscid azimuthal velocity spins up via mutual friction on a combination of the mutual friction and Ekman time scales. The spin-up process is studied in spheres, cylinders (with co- and counter-rotating lids), and cones, and occurs faster in spheres and cones. Ekman pumping is (anti-) clockwise adjacent to the (lower-) upper-right boundary, and mirrored about the rotation axis. The solution obtained by Reisenegger (J. Low Temp. Phys., vol. 92, 1993, p. 77) between slowly accelerating parallel plates is recovered in the associated limit. The hydrodynamic torque on the container decays dual-exponentially with time in a cylinder but not in a sphere or cone, where it is a superposition of exponentials whose time scales vary with latitude. The torque is a good diagnostic of the flow; e.g. it is steepest initially in a sphere with strong mutual friction.
Casimir cascades in two-dimensional turbulence
Authors: Bowman John C. 
Published: July 2013

AbstractIn addition to conserving energy and enstrophy, the nonlinear terms of the two-dimensional incompressible Navier–Stokes equation are well known to conserve the global integral of any continuously differentiable function of the scalar vorticity field. However, the phenomenological role of these additional inviscid invariants, including the issue as to whether they cascade to large or small scales, is an open question. In this work, well-resolved implicitly dealiased pseudospectral simulations suggest that the fourth power of the vorticity cascades to small scales.
Beam Patterns of the Five-hundred-metre Aperture Spherical Telescope: Optimisation
Authors: Dong B. , Han J. L. 
Published: July 2013

AbstractThe Five-hundred-metre Aperture Spherical Telescope (FAST) uses adaptive spherical panels to achieve a huge collecting area for radio waves. In this paper, we try to explore the optimal parameters for the curvature radius of spherical panels and the focal distance by comparison of the calculated beam patterns. We show that to get the best beam shape and maximum gain, the optimal curvature radius of panels is around 300 m, and a small shift in the focal distance of a few cm is needed. The aperture efficiency can be improved by ~10% at 3 GHz by this small shift. We also try to optimise the panel positioning for the best beam, and find that panel shifts of a few mm can improve the beam pattern by a similar extent. Our results indicate that accurate control of the feed and panel positions to the mm level is very crucial for the stability of FAST's observational performance.
Effect of polydextrose and soluble maize fibre on energy metabolism, metabolic profile and appetite control in overweight men and women
Authors: Konings Ellen , Schoffelen Paul F. , Stegen Jos , Blaak Ellen E. 
Published: July 2013

High-fibre diets offer several beneficial health effects. The objective of the present study was to investigate whether replacement of 30 % of the available carbohydrates with polydextrose (PDX) or soluble maize fibre (SCF) at breakfast and lunch would result in an increased fat oxidation rate and satiety, which may be of relevance for body weight control and diabetes prevention. In a single-blind, randomised cross-over study, eighteen overweight men and women underwent four different dietary interventions, which consisted of a PDX diet, a SCF diet and two control diets (full energetic and isoenergetic, comparable with PDX with respect to g or energy percentage of macronutrients, respectively). Glycaemic profile, energy expenditure and substrate oxidation were measured for 24 h in a respiration chamber. Circulating insulin, NEFA and TAG concentrations were determined over a 14 h period during daytime. Appetite ratings were assessed using visual analogue scales. The replacement of available carbohydrates with PDX or SCF reduced the peak glucose response, which was accompanied by reduced postprandial insulin responses. Moreover, higher concentrations of circulating NEFA were observed after consumption of both fibre diets, which were accompanied by an increased fat oxidation over 24 h. This effect was mainly attributed to the lower energetic value of the fibre diets and not to the fibresper se. Besides increasing fat oxidation, PDX exerted a pronounced suppressive effect on appetite ratings. The replacement of available carbohydrates with PDX may be of special interest because of its beneficial effects on metabolic profile and it may affect body weight control in the long term.
Coral tissue mortality of the coral Cladocora caespitosa caused by gastropod Coralliophila meyendorffi in the Mljet National Park (eastern Adriatic Sea)
Authors: Kružić Petar , Sršen Pavica , Cetinić Katarina , Zavodnik Dušan 
Published: July 2013

The corallivorous gastropod Coralliophila meyendorffi (family Coralliophilidae) is a well-known predator of the coral Cladocora caespitosa, particularly in the eastern Adriatic Sea, where population outbreaks can drastically reduce coral cover. Coralliophila meyendorffi ranges in size from 5 to 40 mm in shell total length, and smaller specimens are often found living with the coral C. caespitosa. Specimens of C. meyendorffi feed exclusively on live coral tissue, stripping it from the calcium carbonate skeleton. Recent outbreaks of the gastropod C. meyendorffi have caused considerable damage on a C. caespitosa bank in Veliko Jezero (the Mljet National Park, eastern Adriatic Sea). The bank occurs at depths between 6 and 18 m, covering a 65 m2 area. During summer of 2010, a substantial outbreak affected about 5%t of the coral colonies. The gastropods were highly aggregated in sheltered areas of the coral bank (up to 41 specimens/dm2) with smaller clusters (from 8 to 12 specimens/dm2) on coral colonies that were influenced by strong bottom currents (up to 1.23 ms−1).
Charge regulation in biomolecular solution
Authors: Lund Mikael , Jönsson Bo 
Published: July 2013

AbstractProteins and other biomolecules contain acidic and basic titratable groups that give rise to intricate charge distributions and control electrostatic interactions. ‘Charge regulation’ concerns how the proton equilibria of these sites are perturbed when approached by alien molecular matter such as other proteins, surfaces and membranes, DNA, polyelectrolytes etc. Importantly, this perturbation generates a charge response that leads to attractive intermolecular interactions that can be conveniently described by a single molecular property – the charge capacitance. The capacitance quantifies molecular charge fluctuations, i.e. it is the variance of the mean charge and is anintrinsicproperty on par with the net charge and the dipole moment. It directly enters the free energy expression for intermolecular interactions and can be obtained experimentally from the derivative of the titration curve or theoretically from simulations. In this review, we focus on the capacitance concept as a predictive parameter for charge regulation and demonstrate how it can be used to estimate the interaction of a protein with other proteins, polyelectrolytes, membranes as well as with ligands.
Predation ecology of large sympatric carnivores as influenced by available wild ungulate prey in a tropical deciduous forest of Central India
Authors: Majumder Aniruddha , Sankar Kalyanasundaram , Qureshi Qamar , Basu Santanu 
Published: July 2013

Abstract:Predation ecology of large sympatric carnivores, tiger, leopard and dhole, as influenced by available wild ungulates, chital, sambar, nilgai, gaur and wild pig, were studied between January 2007 and June 2010 in a tropical deciduous forest of Central India. Both line transect and vehicle transect methods were used to estimate population structure and sex ratios of wild ungulates. The observed adult sex ratio (female:male) was found to be skewed towards females for all ungulates. A total of 123 tiger kills were recorded in the cold season and 162 in the hot season, 32 leopard kills were recorded during the cold season and 48 during the hot season and 32 dhole kills were recorded in the cold season and 55 in the hot season. The age–sex class distribution of each ungulate species in the kill data was compared with the corresponding population age–sex distribution recorded from line transects and vehicle transects. All three large carnivores preferred medium-sized prey species, e.g. chital. A significant difference was observed when comparing different health conditions of prey species predated by the three large carnivores. The observed difference in prey choice as per their body size is a strategy adopted by large carnivores to partition prey resources, thus increasing the potential to avoid intra-guild competition.
Mobile Phone Use among Migrant Factory Workers in South China: Technologies of Power and Resistance
Authors: Peng Yinni , Choi Susanne Y. P. 
Published: July 2013

AbstractComparing ethnographic and interview data in three contrasting production arrangements in a labour-intensive factory in South China, this article argues that while the mobile phone constitutes a new contested terrain on the shop floor and facilitates control and resistance between capital and labour simultaneously, the dynamics of control and resistance is contingent upon the exact arrangements of production. While the management strictly prohibit line operators in the assembly line department from using their mobile phones, they turn a blind eye towards mobile phone use among workers in the hardware department, and mandate mobile workers who are not fixed at work stations in both departments to use mobile phones. Diverse managerial control tactics have generated different patterns of worker resistance. Workers in the assembly line department employ strategies to evade managerial surveillance and continue to use mobile phones at work covertly. They also contest the double standards of mobile phone use displayed by the management. Workers in the hardware department challenge the boundaries of legitimate mobile phone use, and mobile workers use tactics to escape being tracked down by the management via their mobile phones. Mobile phones also facilitate the strategy of resistance through exit among all workers.
The coexistence of engineering meanings of function: Four responses and their methodological implications
Authors: Vermaas Pieter E. 
Published: July 2013

AbstractIn this position paper, the ambiguity of functional descriptions in engineering is considered from a methodological point of view. Four responses to this ambiguity are discussed, ranging from defining a single meaning of function and rejecting the different meanings that are currently used in engineering to accepting these meanings as coexisting in engineering and taking function as a family resemblance concept. Rejecting the different meanings is described as the straightforward response to resolving the ambiguity of functional descriptions, yet in engineering research and design methodology it rather seems to be accepted that engineers do use the coexisting meanings side by side. In this paper, explanations are given of why this practice is beneficial to engineering. Then it is explored how the particular meaning that engineers attach to function depends on the tasks for which functional descriptions are used. Finally, the methodological implications of the four responses to the ambiguity of functional descriptions are discussed.
The function analysis diagram: Intended benefits and coexistence with other functional models
Authors: Aurisicchio Marco , Bracewell Rob , Armstrong Gareth 
Published: July 2013

AbstractUnderstanding product functions is a key aspect of the work undertaken by engineers involved in complex system design. The support offered to these engineers by existing modeling tools such as thefunction treeand thefunction structureis limited because they are not intuitive and do not scale well to deal with real-world engineering problems. A research collaboration between two universities and a major power system company in the aerospace domain has allowed the authors to further develop a method for function analysis known as function analysis diagram that was already in use by line engineers. The capability to generate and edit these diagrams was implemented in the Decision Rationale editor, a software tool for capturing design rationale. This article presents the intended benefits of the method and justifies them using an engineering case study. The results of the research have shown that the function analysis diagram method has a simple notation, permits the modeling of product functions together with structure, allows the generation of rich and accurate descriptions of product functionality, is useful to work with variant and adaptive design tasks, and can coexist with other functional modeling methods.
Longitudinal relations between sectarian and nonsectarian community violence and child adjustment in Northern Ireland
Authors: Cummings E. Mark , Merrilees Christine E. , Taylor Laura K. , Shirlow Peter , Goeke-Morey Marcie C. , Cairns Ed 
Published: July 2013

AbstractAlthough relations between political violence and child adjustment are well documented, longitudinal research is needed to adequately address the many questions remaining about the contexts and developmental trajectories underlying the effects on children in areas of political violence. The study examined the relations between sectarian and nonsectarian community violence and adolescent adjustment problems over 4 consecutive years. Participants included 999 mother–child dyads (482 boys, 517 girls),Mages = 12.18 (SD= 1.82), 13.24 (SD= 1.83), 13.61 (SD= 1.99), and 14.66 (SD= 1.96) years, respectively, living in socially deprived neighborhoods in Belfast, Northern Ireland, a context of historical and ongoing political violence. In examining trajectories of adjustment problems, including youth experience with both sectarian and nonsectarian antisocial behaviors, sectarian antisocial behavior significantly predicted more adjustment problems across the 4 years of the study. Experiencing sectarian antisocial behavior was related to increased adolescent adjustment problems, and this relationship was accentuated in neighborhoods characterized by higher crime rates. The discussion considers the implications for further validating the distinction between sectarian and nonsectarian violence, including consideration of neighborhood crime levels, from the child's perspective in a setting of political violence.
Predicting internalizing problems in Chinese children: The unique and interactive effects of parenting and child temperament
Authors: Muhtadie Luma , Zhou Qing , Eisenberg Nancy , Wang Yun 
Published: July 2013

AbstractThe additive and interactive relations of parenting styles (authoritative and authoritarian parenting) and child temperament (anger/frustration, sadness, and effortful control) to children's internalizing problems were examined in a 3.8-year longitudinal study of 425 Chinese children (aged 6–9 years) from Beijing. At Wave 1, parents self-reported on their parenting styles, and parents and teachers rated child temperament. At Wave 2, parents, teachers, and children rated children's internalizing problems. Structural equation modeling indicated that the main effect of authoritative parenting and the interactions of Authoritarian Parenting × Effortful Control and Authoritative Parenting × Anger/Frustration (parents' reports only) prospectively and uniquely predicted internalizing problems. The above results did not vary by child sex and remained significant after controlling for co-occurring externalizing problems. These findings suggest that (a) children with low effortful control may be particularly susceptible to the adverse effect of authoritarian parenting and (b) the benefit of authoritative parenting may be especially important for children with high anger/frustration.