bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Single Amino Acid Mutation (I1012F) of the RNA Polymerase of Marine Viral Hemorrhagic Septicemia Virus Changes
In Vitro
Virulence to Rainbow Trout Gill Epithelial Cells
Authors: Kim Sung-Hyun , Thu Beate J. , Skall Helle F. , Vendramin Niccolò , Evensen Øystein 
Published: April 2014

ABSTRACT

Viral hemorrhagic septicemia virus (VHSV) is separated into four different genotypes (I to IV) with different sublineages (K. Einer-Jensen, P. Ahrens, R. Forsberg, and N. Lorenzen, J. Gen. Virol. 85:1167–1179, 2004; K. Einer-Jensen, J. Winton, and N. Lorenzen, Vet. Microbiol. 106:167–178, 2005). European marine VHSV strains (of genotypes I to III) are, in general, nonpathogenic or have very low pathogenicity to rainbow trout after a waterborne challenge, and here we also show that genotype IVa is nonpathogenic to trout. Despite several attempts, it has not been possible to link genomic variation to
in vivo
virulence.
In vitro
virulence to gill epithelial cells (GECs) has been used as a proxy for
in vivo
virulence, and here we extend these studies further with the purpose of identifying residues associated with
in vitro
virulence. Genotype Ia (DK-3592B) and III (NO/650/07) isolates, which are pathogenic to rainbow trout (O. B. Dale, I. Orpetveit, T. M. Lyngstad, S. Kahns, H. F. Skall, N. J. Olesen, and B. H. Dannevig, Dis. Aquat. Organ. 85:93–103, 2009), were compared to two marine strains that are nonpathogenic to trout, genotypes Ib (strain 1p8 [H. F. Mortensen, O. E. Heuer, N. Lorenzen, L. Otte, and N. J. Olesen, Virus Res. 63:95–106, 1999]) and IVa (JF-09). DK-3592 and NO/650/07 were pathogenic to GECs, while marine strains 1p8 and JF-09 were nonpathogenic to GECs. Eight conserved amino acid substitutions contrasting high- and low-virulence strains were identified, and reverse genetics was used in a gain-of-virulence approach based on the JF-09 backbone. Mutations were introduced into the G, NV, and L genes, and seven different virus clones were obtained. For the first time, we show that a single amino acid mutation in conserved region IV of the L protein, I1012F, rendered the virus able to replicate and induce a cytopathic effect in trout GECs. The other six mutated variants remained nonpathogenic.

IMPORTANCE
This is the first study to clearly link
in vitro
virulence of viral hemorrhagic septicemia virus (VHSV) with an amino acid residue in the L protein, a site located in conserved region IV of the L protein.
In vitro
virulence is documented by induction of cytopathic effects and viability studies of gill epithelial cells, and the observed cellular responses to infection are associated with increased viral replication levels. There are no previous studies addressing the importance of the L protein or the RNA-dependent RNA polymerase for virus virulence
in vitro
or
in vivo
. Therefore, the findings reported here should broaden the search for pathogenicity traits in novirhabdoviruses, and there is a possibility that the polymerase participates in defining the host species virulence of various VHSV strains.
Kaposi's Sarcoma-Associated Herpesvirus ORF45 Mediates Transcriptional Activation of the HIV-1 Long Terminal Repeat via RSK2
Authors: Karijolich John , Zhao Yang , Peterson Bret , Zhou Qiang , Glaunsinger Britt 
Published: April 2014

ABSTRACTRobust activation of human immunodeficiency virus type 1 (HIV-1) gene expression occurs upon superinfection with Kaposi's sarcoma-associated herpesvirus (KSHV), a common AIDS-associated pathogen. Though the mechanisms underlying this phenotype remain unknown, several KSHV-encoded factors have been reported to stimulate HIV-1 long terminal repeat (LTR) activity. Here, we systematically evaluated the ability of KSHV tegument proteins to modulate the activation of an integrated HIV-1 LTR and revealed that the most potent individual activator is ORF45. ORF45 directs an increase in RNA polymerase II recruitment to the HIV-1 LTR, leading to enhanced transcriptional output. ORF45 is a robust activator of the p90 ribosomal S6 kinases (RSK), and we found that this activity is necessary but not sufficient to increase transcription from the LTR. Of the three widely expressed RSK isoforms, RSK2 appears to be selectively involved in LTR stimulation by both KSHV ORF45 and HIV-1 Tat. However, constitutively active RSK2 is unable to stimulate the LTR, suggesting that ORF45 may preferentially direct this kinase to a specific set of targets. Collectively, our findings reveal a novel transcriptional activation function for KSHV ORF45 and highlight the importance of RSK2 in shaping the transcriptional environment during infection.IMPORTANCEKaposi's sarcoma-associated herpesvirus (KSHV) is a prominent AIDS-associated pathogen. Previous studies have shown that infection of cells containing human immunodeficiency virus type 1 (HIV-1) with KSHV leads to potent stimulation of HIV-1 gene expression by activating the HIV-1 promoter, termed the long terminal repeat (LTR). Here, we compared the abilities of various KSHV proteins to activate gene expression from the HIV-1 LTR and found that KSHV ORF45 is the most potent activator. ORF45 is known to induce cell signaling through ribosomal S6 kinase (RSK) and enhance protein translation. However, we revealed that the activation of a specific isoform of RSK by ORF45 also leads to increased mRNA synthesis from the LTR by the host RNA polymerase. Collectively, our findings provide new insight into the interviral interactions between KSHV and HIV that may ultimately impact disease.
Erosion in the Chilean Andes between 27°S and 39°S: tectonic, climatic and geomorphic control
Authors: Carretier S. , Tolorza V. , Rodríguez M. P. , Pepin E. , Aguilar G. , Regard V. , Martinod J. , Riquelme R. , Bonnet S. , Brichau S. , Hérail G. , Pinto L. , Farías M. , Charrier R. , Guyot J. L. 
Published: April 2014

Abstract

The effect of mean precipitation rate on erosion is debated. Three hypotheses may explain why the current erosion rate and runoff may be spatially uncorrelated: (1) the topography has reached a steady state for which the erosion rate pattern is determined by the uplift rate pattern; (2) the erosion rate only depends weakly on runoff; or (3) the studied catchments are experiencing different transient adjustments to uplift or to climate variations. In the Chilean Andes, between 27°S and 39°S, the mean annual runoff rates increase southwards from 0.01 to 2.6 m a
−1
but the catchment averaged rates of decadal erosion (suspended sediment) and millennial erosion (
10
Be in river sand) peak at
c.
0.25 mm a
−1
for runoff
c.
0.5 m a
−1
and then decrease while runoff keeps increasing. Erosion rates increase non-linearly with the slope and weakly with the square root of the runoff. However, sediments trapped in the subduction trench suggest a correlation between the current runoff pattern and erosion over millions of years. The third hypothesis above may explain these different erosion rate patterns; the patterns seem consistent with, although not limited to, a model where the relief and erosion rate have first increased and then decreased in response to a period of uplift, at rates controlled by the mean precipitation rate.
Transferring from clopidogrel loading dose to prasugrel loading dose in acute coronary syndrome patients
Authors: Saucedo Jorge F. , Cardillo Tracy E. , Jakubowski Joseph A. , Henneges Carsten , Effron Mark B. , Lipkin Fred R. , Walker Joseph R. , Duvvuru Suman , Sundseth Scott S. , Fisher Harold N. , Angiolillo Dominick J. , Diodati Jean G. 
Published: April 2014

SummaryHigh on-treatment platelet reactivity (HPR) has been identified as an independent risk factor for ischaemic events. The randomised, doubleblind, TRIPLET trial included a pre-defined comparison of HPR in acute coronary syndrome (ACS) patients undergoing percutaneous coronary intervention (PCI) following a placebo/600-mg clopidogrel loading dose (LD) immediately before a subsequent prasugrel 60-mg or 30-mg LD. Platelet reactivity was assessed using the VerifyNow® P2Y12 assay (P2Y12 Reaction Units, PRU) within 24 hours (h) following the placebo/clopidogrel LD (immediately prior to prasugrel LD), and at 2, 6, 24, 72 h following prasugrel LDs. The impact of CYP2C19 predicted metaboliser phenotype (extensive metaboliser [EM] and reduced metabolisers [RM]) on HPR status was also assessed. HPR (PRU ≥240) following the clopidogrel LD (prior to the prasugrel LD) was 58.5% in the combined clopidogrel LD groups. No significant difference was noted when stratified by time between the clopidogrel and prasugrel LDs (≤6 hs vs >6 h). At 6 h following the 2nd loading dose in the combined prasugrel LD groups, HPR was 7.1%, with 0% HPR by 72 h. There was no significant effect of CYP2C19 genotype on pharmacodynamic (PD) response following either prasugrel LD treatments at any time point, regardless of whether it was preceded by a clopidogrel 600-mg LD. In conclusion, in this study, patients with ACS intended for PCI showed a high prevalence of HPR after clopidogrel 600-mg LD regardless of metaboliser status. When prasugrel LD was added, HPR decreased substantially by 6 h, and was not seen by 72 h.
New Perspectives on Factor Markets and Ancient Middle Eastern Economies: A Survey
Authors: van Bavel Bas 
Published: April 2014

AbstractThe conventional view of markets for land, labour, and capital as a modern, Western phenomenon is questionable. Factor markets did indeed exist in Iraq, and even thrived, in various parts of its pre-modern history, including the period around 2000bce, the “long” sixth century (c. 620-480bce), and the eighth and ninth centuriesce. By employing the long-term approach used in this issue ofjeshoand by placing the organization of these markets in their wider social-political context, we can understand better how these markets developed, how they functioned, and why they rose and declined again.
Factor Markets in Hellenistic and Parthian Babylonia (331 BCE-224 CE)
Authors: van der Spek Robartus J. 
Published: April 2014

AbstractBecause the evidence is meagre, this article takes a qualitative rather than a quantitative approach to the markets of land, labour, and capital in Hellenistic and Parthian Babylonia. The evidence consists of administrative documents from Babylon and Uruk (Babylonian clay tablets in cuneiform script), from Dura Europos on parchment or papyrus (in Greek and Aramaic), and from Avroman in (Greek and Pahlavi). These texts suggest that the land market was restricted by the legal rights of king and temple. There is little information on wage labour. Slave labour existed, but neither its role in the economy nor the importance of the slave trade has been adequately assessed. The use of credit, interest, cheques, and other financial instruments is attested, but its significance is small, not least because of the prevalence of iconic interest rates of 20%, set by tradition rather than by market forces. As far as the evidence goes, the markets for land, labour, and capital were restricted by tradition and by the claims of king and temples on land, while the commodity markets were much more market-oriented.
Factor Markets in Early Islamic Iraq, c. 600-1100 AD
Authors: van Bavel Bas , Campopiano Michele , Dijkman Jessica 
Published: April 2014

AbstractThis paper reconstructs the organization and development of factor markets in early medieval Iraq. It shows that from the late Sasanian period on, and accelerating in the early Islamic period, there was a relatively unrestricted functioning of markets for goods, labour, and capital. This stimulated market exchange, associated with growing monetization of the economy, especially in the towns, but also in the countryside, even though coercion remained more pronounced there. We hypothesize that these developments brought economic dynamism but simultaneously increased inequality and furthered the rise of new, powerful elite groups, causing the decline of the same markets.
A Squall-Line-Like Principal Rainband in Typhoon Hagupit (2008) Observed by Airborne Doppler Radar
Authors: Tang Xiaowen , Lee Wen-Chau , Bell Michael 
Published: April 2014

Abstract
This study examines the structure and dynamics of Typhoon Hagupit’s (2008) principal rainband using airborne radar and dropsonde observations. The convection in Hagupit’s principal rainband was organized into a well-defined line with trailing stratiform precipitation on the inner side. Individual convective cells had intense updrafts and downdrafts and were aligned in a wavelike pattern along the line. The line-averaged vertical cross section possessed a slightly inward-tilting convective core and two branches of low-level inflow feeding the convection. The result of a thermodynamic retrieval showed a pronounced cold pool behind the convective line. The horizontal and vertical structures of this principal rainband show characteristics that are different than the existing conceptual model and are more similar to squall lines and outer rainbands.
The unique convective structure of Hagupit’s principal rainband was associated with veering low-level vertical wind shear and large convective instability in the environment. A quantitative assessment of the cold pool strength showed that it was quasi balanced with that of the low-level vertical wind shear. The balanced state and the structural characteristics of convection in Hagupit’s principal rainband were dynamically consistent with the theory of cold pool dynamics widely applied to strong and long-lived squall lines. The analyses suggest that cold pool dynamics played a role in determining the principal rainband structure in addition to storm-scale vortex dynamics.
Evaluating Skill of Seasonal Precipitation and Temperature Predictions of NCEP CFSv2 Forecasts over 17 Hydroclimatic Regions in China
Authors: Lang Yang , Ye Aizhong , Gong Wei , Miao Chiyuan , Di Zhenhua , Xu Jing , Liu Yu , Luo Lifeng , Duan Qingyun 
Published: April 2014

Abstract
Seasonal predictions of precipitation and surface air temperature from the Climate Forecast System, version 2 (CFSv2), are evaluated against gridded daily observations from 1982 to 2007 over 17 hydroclimatic regions in China. The seasonal predictive skill is quantified with skill scores including correlation coefficient, RMSE, and mean bias for spatially averaged seasonal precipitation and temperature forecasts for each region. The evaluation focuses on identifying regions and seasons where significant skill exists, thus potentially contributing to skill in hydrological prediction. The authors find that the predictive skill of CFSv2 precipitation and temperature forecasts has a stronger dependence on seasons and regions than on lead times. Both temperature and precipitation forecasts show higher skill from late summer [July–September (JAS)] to late autumn [October–December (OND)] and from winter [December–February (DJF)] to spring [March–May (MAM)]. The skill of CFSv2 precipitation forecasts is low during summer [June–August (JJA)] and winter (DJF) over all of China because of low potential predictability of the East Asian summer monsoon and the East Asian winter monsoon for China. As expected, temperature predictive skill is much higher than precipitation predictive skill in all regions. As observed precipitation shows significant correlation with the Oceanic Niño index over western, southwestern, and central China, the authors found that CFSv2 precipitation forecasts generally show similar correlation pattern, suggesting that CFSv2 precipitation forecasts can capture ENSO signals. This evaluation suggests that using CFSv2 forecasts for seasonal hydrological prediction over China is promising and challenging.
Correcting laboratory results for the effects of interferences: an approach incorporating uncertainty of measurement
Authors: Jones Graham RD , Hawkins Robert C 
Published: April 2014

Background Results of numerical pathology tests may be subject to interference and many laboratories identify such interferences and withhold results or issue warnings if clinically erroneous results may be issued. Some laboratories choose to correct for the effect of interferences, with the uncertainty of the correction noted as a limitation in this process. We investigate the effect of correcting for the effect of interferences on the ability to release results within defined error goals using the effect of in-vitro haemolysis on serum potassium measurement as an example. Methods A model was developed to determine the uncertainty of a result corrected for the effect of an interferent with a linear relationship between concentration and effect. The model was used to assess the effect of correction on the results which could be released within specified accuracy criteria. Results Using the effects of haemolysis on potassium results as an example, the maximum amount of haemolysis in a sample that would change the result by > 0.5 mmol/L, with a frequency of 5%, was increased from approximately 1100 mg/L (no correction) to 8000 mg/L (with correction). Conclusions With modelling of the factors related to the uncertainties of results in the presence of interferences, it is possible to release results in the presence of significantly higher concentrations of interferences after correction than without correction. Correction of a result for a known bias and allowance for the uncertainty of the correction can be considered consistent with the guide to the expression of uncertainty in measurement (GUM).
How is dilated cardiomyopathy investigated in Scotland? A contemporary survey
Authors: Weir Robin AP , Simpson Joanne , Petrie Colin J 
Published: April 2014

Background and aims Dilated cardiomyopathy (DCM) is a common cause of heart failure. The underlying aetiology remains poorly characterised, with ca. 50% labelled ‘idiopathic’. We assessed the extent to which the aetiology of DCM is investigated in Scotland, in comparison to European Society of Cardiology (ESC) recommendations. Methods and results Questionnaires regarding the use of coronary angiography, use and availability of cardiac magnetic resonance imaging (CMR) and blood/urine panels to investigate the causes of DCM were sent to the heart failure lead in each of the 23 hospitals across Scotland with an established cardiology department; responses were obtained from 21/23 (91.3%). ESC guidelines regarding coronary angiography were adopted in only 8/21 (38.1%). Only 7/21 (33.3%) had easy access to CMR although 14/21 (66.7%) felt it would be a useful test in DCM. The ESC-recommended blood profile was checked routinely in 7/21 (33.3%). Additional blood tests, many of which not currently recommended, were performed in selected centres. Conclusions DCM patients in Scotland are in general unlikely to undergo current ESC-recommended investigation into the underlying aetiology. There is a need for prospective studies to determine the success rate and influence on management and outcome of such multifaceted approaches to investigating the cause of DCM.
Inaccuracy of transthoracic echocardiography for the identification of right‐sided vegetation in patients with no history of intravenous drug abuse or cardiac device insertion
Authors: Xie Jiang , Liu Shuang , Yang Jinghua , Xu Jie , Zhu Guangfa 
Published: April 2014

Objective: The use of transthoracic echocardiography (TTE) to identify right-sided infective endocarditis (RSIE) vegetation is controversial. Data are scarce for patients with no history of intravenous drug abuse (IVDA) or cardiac device insertion. This study analysed the consistency of presurgical echocardiographic results with surgical findings for vegetation identification, and the factors that influence accuracy of echocardiography. Methods: This retrospective trial divided infective endocarditis (IE) patients into three subgroups according to the results of their presurgical TTE: left-sided native IE (LSNIE), left-sided prosthetic valve IE (LSPIE) and RSIE. The accuracy of TTE was tested by comparing vegetation (number and location), detected presurgery by TTE, with actual findings during surgery. Results: In total, 416 patients were analysed, 322 with LSNIE, 31 with LSPIE and 63 with RSIE. Consistency between TTE findings and surgical results was lower in the RSIE group compared with the LSPIE and LSNIE groups. Consistency was lowered by the presence of vegetation in multiple locations and atypical distribution – both of which were increased in the RSIE group. The chance of vegetation in both sides of the heart rose with increased numbers of vegetation locations in RSIE patients. A high proportion of RSIE patients had congenital heart defects, mostly ventricular septal defects. Conclusions: TTE may be unsuitable for RSIE patients with no history of IVDA or cardiac device insertion, because multifocal and atypically distributed vegetation may influence detection accuracy.
Retrograde Intramedullary Nail With Femoral Head Allograft for Large Deficit Tibiotalocalcaneal Arthrodesis
Authors: Bussewitz Bradly , DeVries J. George , Dujela Michael , McAlister Jeffrey E. , Hyer Christopher F. , Berlet Gregory C. 
Published: April 2014

Background: Large bone defects present a difficult task for surgeons when performing single-stage, complex combined hindfoot and ankle reconstruction. There exist little data in a case series format to evaluate the use of frozen femoral head allograft during tibiotalocalcaneal arthrodesis in various populations in the literature. Methods: The authors evaluated 25 patients from 2003 to 2011 who required a femoral head allograft and an intramedullary nail. The average time of final follow-up visit was 83 ± 63.6 weeks (range, 10-265). Results: Twelve patients healed the fusion (48%). Twenty-one patients resulted in a braceable limb (84%). Four patients resulted in major amputation (16%). Conclusion: This series may allow surgeons to more accurately predict the success and clinical outcome of these challenging cases. Level of Evidence: Level IV, case series.
Judicial Independence and Opinion Clarity on State Supreme Courts
Authors: Goelzhauser Greg , Cann Damon M. 
Published: April 2014

AbstractWe contribute to the literature on judicial independence and performance in the states by analyzing opinion clarity. Written opinions are the primary means of communication for state supreme court justices, and clarity is a core component of judicial performance. Using automated text analysis on a sample of state supreme court opinions from all 50 states, we find that variation in judicial retention systems is not associated with substantively meaningful differences in opinion clarity. Furthermore, elected judges do not seem to produce clearer opinions in salient cases notwithstanding the increased public visibility of those decisions. These results suggest that judges tend to conform to prevailing professional norms despite differing institutional pressures.
Reevaluating the Effects of Redistricting on Electoral Competition, 1972–2012
Authors: Carson Jamie L. , Crespin Michael H. , Williamson Ryan D. 
Published: April 2014

AbstractIn this note, we revisit the work of Carson and Crespinto examine the effect of different redistricting plans on competitiveness in U.S. House elections. Similar to the previous results, our probit estimates on an expanded dataset that includes redistricting cycles from 1972 to 2012 reveal that commission and court-drawn districts experience more competition on average than those drawn by legislatures. These results provide additional support for the hypothesis that one way to increase the competitiveness of congressional elections is to allow extra-legislative bodies to draw congressional district boundaries.