bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loss of PI5P4Kα slows the progression of aPtenmutant basal cell model of prostate cancer
Authors: Triscott Joanna , Lehner Marika , Benjak Andrej , Reist Matthias , Emerling Brooke M. , Ng Charlotte K.Y. , de Brot Simone , Rubin Mark A. 
Published: August 2024

ABSTRACTWhile early prostate cancer (PCa) depends on the androgen receptor (AR) signaling pathway, which is predominant in luminal cells, there is much to be understood about the contribution of epithelial basal cells in cancer progression. Herein, we observe cell-type specific differences in the importance of the metabolic enzyme phosphatidylinositol 5-phosphate 4-kinase alpha (PI5P4Kα; gene namePIP4K2A) in the prostate epithelium. We report the development of a basal-cell-specific genetically engineered mouse model (GEMM) targetingPip4k2aalone or in combination with the tumor suppressor phosphatase and tensin homolog (Pten). PI5P4Kα is enriched in basal cells, and no major histopathological changes were detectable following gene deletion. Notably, the combined loss ofPip4k2aslowed the development ofPtenmutant mouse prostatic intraepithelial neoplasia (mPIN). Through the inclusion of a lineage tracing reporter, we utilize single-cell RNA sequencing to evaluate changes resulting fromin vivodownregulation ofPip4k2aand characterize cell populations influenced in the established Probasin-Cre and Cytokeratin 5 (CK5)- Cre driven GEMMs. Transcriptomic pathway analysis points towards the disruption of lipid metabolism as a mechanism for reduced tumor progression. This was functionally supported by shifts of carnitine lipids in LNCaP PCa cells treated withsiPIP4K2A. Overall, these data nominate PI5P4Kα as a target for PTEN mutant PCa.One Sentence SummaryLoss of PI5P4Kα slows cancer progression in prostate basal cells.
VARIETAL SCREENING OF BLACKGRAM GENOTYPES AGAINST CERCOSPORA LEAF SPOT (Cercospora canescens)
Authors: Shrestha Siddhanta , Bohara Sinchan , Oli Sarita , Nainabasti Anjal , Bohara Khem Raj , Upadhyaya Kriti , Basnet Indira 
Published: August 2024

AbstractScreening of 12 blackgram genotypes against Cercospora Leaf Spot (Cercospora canescens) was carried out in RCB design with 3 replications in research plot of Mid-West Academy and Research Institute, Tulsipur, Dang during Bhadra to Mangsir, 2078 B.S. The blackgram genotypes were brought from Grain Legumes Research Program, Khajura, Banke. Disease severity was taken 3 times at 40, 47, and 54 days after sowing. Disease scoring was done as a percentage of leaf area infected on the individual plant at 7-day intervals and disease incidence, disease severity, mean AUDPC, and mean yield was calculated. Disease incidence was not significant among the tested genotypes. Disease severity at 40, 47, and 54 DAS was highly significant among the genotypes. Mean disease score and mean area under disease progressive curve (AUDPC) were also highly significant. Among the genotypes, 10 genotypes were categorized as moderately resistant and 2 genotypes (BLG 0066-1-1 and BLG 0035-1) were categorized as moderately susceptible. The highest Mean AUDPC value (324.1) was possessed by BLG 0035-1 followed by BLG 0066-1-1 (317.6). The lowest mean AUDPC value (175) was possessed by BLG 0069-1. A highly significant difference was found in yield among the black gram genotypes. The highest yield (799 kg/ha) by obtained by BLG 0068-2 followed by Rampur mas (769 kg/ha). The lowest yield (495 kg/ha) was obtained by BLG 0066-1.
Regulative synthesis of capsular polysaccharides in the pathogenesis ofStreptococcus suis
Authors: Wang Xingye , Wang Jie , Li Ning , Fan Xin , Wang Beinan 
Published: August 2024

AbstractStreptococcus suis(S. suis) is an important zoonotic pathogen causing substantial economic losses in the swine industry.S. suisserotype 2 (SS2) is often isolated from the diseased.S. suisexpresses capsular polysaccharide (CPS), a virulence factor crucial for their survival in the blood. However, the role of CPS in the pathogenesis ofS. suisis incomplete. Here, we showed a dynamic regulation of CPS inS. suispathogenesis. In a mouse infection model, an SS2 strain (05ZYH33) was detected in the nasal-associated lymphoid tissue (NALT) and cerebrospinal fluid (CSF) as early as 30 min after intranasal inoculation without bacteremia. Histological analysis revealed that 05ZYH33 in the nasal cavity invaded the olfactory epithelium, resulting in early brain inflammation. Transmission electron microscopy showed that 05ZYH33 isolated from NALT and CSF at early infection time had a thin layer of CPS, and those detected in the blood 5 h post- inoculation showed a much thicker CPS. In addition, adoptive transfer of anti-CPS restricted 05ZYH33 in the blood but not in NALT or CSF. However, an antiserum directed to multiple non-CPS virulence factors (anti-V5) efficiently inhibited 05ZYH33 in NALT, CSF, and blood. Thus, 05ZYH33 colonizes NALT more efficiently without CPS and subsequently invades the meninges through the olfactory nerve system. These findings provide valuable information for the treatment ofS. suisinfection and the development of vaccines across serotypes ofS. suisby targeting CPS-independent immunity.
Diverse somatic Transformer and sex chromosome karyotype pathways regulate gene expression in Drosophila gonad development
Authors: Mahadevaraju Sharvani , Pal Soumitra , Bhaskar Pradeep , McDonald Brennan D. , Benner Leif , Denti Luca , Cozzi Davide , Bonizzoni Paola , Przytycka Teresa M. , Oliver Brian 
Published: August 2024

ABSTRACTThe somatic sex determination genetransformer(tra) is required for the highly sexually dimorphic development of most somatic cells, including those of the gonads. In addition, somatictrais required for the germline development even though it is not required for sex determination within germ cells. Germ cell autonomous gene expression is also necessary for their sex determination. To understand the interplay between these signals, we compared the phenotype and gene expression of larval wild-type gonads and the sex-transformedtragonads. XX larval ovaries transformed into testes were dramatically smaller than wild-type, with significant reductions in germ cell number, likely due to altered geometry of the stem cell niche. Additionally, there was a defect in progression into spermatocyte stages. XY larval testes transformed into ovaries had excessive germ cells, possibly due to the earlier onset of cell division. We suggest that germ cells are neither fully female nor male following somatic sex transformation, with certain pathways characteristic of each sex expressed intramutants. We found multiple patterns of somatic and germline gene expression control exclusively due totra, exclusively due to sex chromosome karyotype, but usually due to a combination of these factors showingtraand sex chromosome karyotype pathways regulate gene expression during Drosophila gonad development.
DNA-PKcs Inhibition Improves Sequential Gene Insertion of the Full-LengthCFTRcDNA in Airway Stem Cells
Authors: Stack Jacob T. , Rayner Rachael E. , Nouri Reza , Suarez Carlos J. , Kim Sun Hee , Kanke Karen L. , Vetter Tatyana A. , Cormet-Boyaka Estelle , Vaidyanathan Sriram 
Published: August 2024

ABSTRACTCystic fibrosis (CF) is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Although many people with CF (pwCF) are treated using CFTR modulators, some are non-responsive due to their genotype or other uncharacterized reasons. Autologous airway stem cell therapies, in which theCFTRcDNA has been replaced, may enable a durable therapy for all pwCF. Previously, CRISPR-Cas9 with two AAVs was used to sequentially insert two halves of theCFTRcDNA and an enrichment cassette into theCFTRlocus. However, the editing efficiency was <10% and required enrichment to restore CFTR function. Further improvement in gene insertion may enhance cell therapy production. To improveCFTRcDNA insertion in human airway basal stem cells (ABCs), we evaluated the use of the small molecules AZD7648 and ART558 which inhibit non-homologous end joining (NHEJ) and micro-homology mediated end joining (MMEJ). Adding AZD7648 alone improved gene insertion by 2-3-fold. Adding both ART558 and AZD7648 improved gene insertion but induced toxicity. ABCs edited in the presence of AZD7648 produced differentiated airway epithelial sheets with restored CFTR function after enrichment. Adding AZD7648 did not increase off-target editing. Further studies are necessary to validate if AZD7648 treatment enriches cells with oncogenic mutations.
Optimising the viscoelastic properties of hyaluronic acid hydrogels through colloidal particle interactions: a response surface methodology approach
Authors: Ramanathan Giriprasath , Hassan Masroora , Rochev Yury 
Published: August 2024

AbstractEnhancing the viscoelastic characteristics of hydrogel systems through strategic colloidal particle interactions is paramount for their functionality in rectal gel applications. This investigation delves into the synergistic interactions between cationic nanoparticles (CNPs), anionic nanoparticles (ANPs), and composite nanoparticles (NPs) within a hyaluronic acid (HA) hydrogel matrix, employing response surface methodology (RSM) for optimisation. Critical parameters, namely the volume fraction of NPs and oscillatory amplitude, were meticulously calibrated to achieve optimal complex viscosity, as determined by advanced rheometric analysis. The findings reveal substantial effects of CNPs, ANPs, and mixed NPs on the viscoelasticity of the HA hydrogel, with complex viscosity measurements of 490.82 ± 10.57, 214.70 ± 8.96, and 328.46 ± 6.67 mPa·s, respectively. The hydrogel system with mixed NPs exhibited a strong concordance with empirical data (R² = 0.9843), validating the predictive precision of the model. Morphological assessments uncovered a highly interconnected network within the HA gel-particle composite, characterised by both densely and sparsely packed porous architectures. This study presents a robust framework for modulating viscoelastic properties in colloidal particle-gel systems, providing pivotal insights for the development of advanced rectal gel formulations.
Extensive Search for Axion Dark Matter over 1 GHz with CAPP’S Main Axion Experiment
Authors: Ahn Saebyeok , Kim JinMyeong , Ivanov Boris I. , Kwon Ohjoon , Byun HeeSu , van Loo Arjan F. , Park SeongTae , Jeong Junu , Lee Soohyung , Kim Jinsu , Kutlu Çağlar , Yi Andrew K. , Nakamura Yasunobu , Oh Seonjeong , Ahn Danho , Bae SungJae , Choi Hyoungsoon , Choi Jihoon , Chong Yonuk , Chung Woohyun , Gkika Violeta , Kim Jihn E. , Kim Younggeun , Ko Byeong Rok , Miceli Lino , Lee Doyu , Lee Jiwon , Lee Ki Woong , Lee MyeongJae , Matlashov Andrei , Parashar Pallavi , Seong Taehyeon , Shin Yun Chang , Uchaikin Sergey V. , Youn SungWoo , Semertzidis Yannis K. 
Published: August 2024

We report an extensive high-sensitivity search for axion dark matter above 1 GHz at the Center for Axion and Precision Physics Research (CAPP). The cavity resonant search, exploiting the coupling between axions and photons, explored the frequency (mass) range of 1.025 GHz (4.24  μeV) to 1.185 GHz (4.91  μeV). We have introduced a number of innovations in this field, demonstrating the practical approach of optimizing all the relevant parameters of axion haloscopes, extending presently available technology. The CAPP 12 T magnet with an aperture of 320 mm made of Nb3Sn and NbTi superconductors surrounding a 37 l ultralight-weight copper cavity is expected to convert Dine-Fischler-Srednicki-Zhitnitsky axions into approximately 102 microwave photons per second. A powerful dilution refrigerator, capable of keeping the core system below 40 mK, combined with quantum-noise-limited readout electronics, achieved a total system noise of about 200 mK or below, which corresponds to a background of roughly 4×103 photons per second within the axion bandwidth. The combination of all those improvements provides unprecedented search performance, imposing the most stringent exclusion limits on axion-photon coupling in this frequency range to date. These results also suggest an experimental capability suitable for highly sensitive searches for axion dark matter above 1 GHz.

Published by the American Physical Society
2024
Artificial intelligence has become your co-worker! Exploring changes related to corporate culture and innovation capability
Authors: Chu Chengxiang , Cheng Sihan , Cao Cong 
Published: August 2024

PurposeThere is currently a gap in the research regarding the effect of corporate culture on corporate innovation capability. Based on cultural hierarchy theory, in this paper, we explore the interactions between cultural factors and innovation capability in emerging market firms (EMFs). We discuss the mechanisms by which incentive, institutional, and vibrant corporate cultures influence corporate innovation capability. Furthermore, we consider the transformation of artificial general intelligence (AGI) from a tool into a colleague and how this affects the relationship between corporate culture and innovation capability.Design/methodology/approachAn online questionnaire was distributed to corporate employees to explore their attitudes towards AGI and corporate culture. In total, 523 valid questionnaires were empirically analysed using partial least squares structural equation modelling and multigroup analysis (MGA).FindingsThe results showed that incentive culture, institutional culture, and vibrant culture had a positive impact on corporate innovation capability. MGA revealed significant differences between employees who considered AGI a tool and those who considered it a colleague. Employees who treated AGI as a colleague were likely to be influenced by a vibrant culture, whereas employees who treated AGI as a tool were likely to be influenced by an incentive or institutional culture.Originality/valueBuilding on cultural hierarchy theory, our study provides a new theoretical framework to enrich current research on the relationship between corporate culture and AGI. The study can help EMF managers adjust incentive and institutional cultures before AGI shifts from being a tool to a colleague and negatively impacts innovation capacity.
The interplay between total quality management (TQM) and organizational learning: similarities or complementarities?
Authors: Tajouri Oumayma , El Manzani Younès , Lakhal Lassaad 
Published: August 2024

Purpose
Previous contributions have reported a reciprocal relationship between total quality management (TQM) and organizational learning (OL). This paper aims to provide a clarified understanding of this relationship.

Design/methodology/approach
A content analysis of 15 studies published in academic journals since 2000 was conducted to examine the reciprocal relationship between TQM and OL. The findings of this analysis, along with potential explanations for bidirectional links, are discussed.

Findings
This paper’s main contribution is to explain the interplay between these two management approaches by sorting the practices, behaviors, and values regarding their focus. The findings have identified two foci precisely common focus and complementary focus.

Originality/value
The relationship between TQM and OL has been examined in previous studies. Whereas, this study examines the reciprocal relationship between these approaches. This review presents new insights into the relationship between these approaches.
Does mandating gender quota in corporate boards affect firms’ credit ratings? Evidence from India
Authors: Muricken Navya J. , Bhagawan Praveen , Mukhopadhyay Jyoti Prasad 
Published: August 2024

PurposeThe purpose of this paper is to examine the impact of compulsory presence of female members due to gender quota on corporate boards on firms’ credit ratings.Design/methodology/approachWe investigate the impact of female directorial appointment on a firm’s credit rating using firm-level panel data in a regression framework with industry- and year-fixed effects to account for unobserved heterogeneity. Further, to address endogeneity, we employ the difference-in-differences (DiD) technique by exploiting the changes in the corporate board composition induced by the exogeneous gender quota regulation. We also employ the Oster (2019) approach to test for omitted variable bias.FindingsIn this paper, we find that the firms that appoint female members on corporate boards post-gender quota mandate (treatment firms) enjoy improved credit ratings as compared to firms that had female members on corporate boards before the gender quota mandate (control group firms) became effective. The findings are robust to alternate definitions of credit rating, treatment and post variables.Originality/valueWe employ an alternative econometric technique, such as Oster’s (2019) specification, to show that the involvement of female directors on corporate boards helps firms in improving firm’s credit ratings. We also identify corporate risk measured using stock return volatility and cash flow volatility as the potential channels through which female directors’ involvement on corporate boards leads to the improvement in firms’ credit ratings.
Determining the optimal food hub location in the fresh produce supply chain
Authors: Ge Houtian , Yi Jing , Goetz Stephan J. , Cleary Rebecca , Gómez Miguel I. 
Published: August 2024

Purpose
Using recent US regional data associated with food system operations, this study aims at building optimization and econometric models to incorporate varying influential factors on food hub location decisions and generate effective facility location solutions.

Design/methodology/approach
Mathematical optimization and econometric models have been commonly used to identify hub location decisions, and each is associated with specific strengths to handle uncertainty. This paper develops an optimization model and a hurdle model of the US fresh produce sector to compare the hub location solutions between these two modeling approaches.

Findings
Econometric modeling and mathematical optimization are complementary approaches. While there is a divergence between the results of the optimization model and the econometric model, the optimization solution is largely confirmed by the econometric solution. A combination of the results of the two models might lead to improved decision-making.

Practical implications
This study suggests a future direction in which model development can move forward, for example, to explore and expose how to make the existing modeling techniques easier to use and more accessible to decision-makers.

Social implications
The models and results provide information that is currently limited and is useful to help inform sustainable decisions of various stakeholders interested in the development of regional food systems, regional infrastructure investment and operational strategies for food hubs.

Originality/value
This study sheds light on how the application of complementary modeling approaches improves the effectiveness of facility location solutions. This study offers new perspectives on elaborating key features to encompass facility location issues by applying interdisciplinary approaches.
The impact of digitalization on promoting business during crisis: evidence from Armenian SMEs
Authors: Beglaryan Mane , Drampyan Anush , Sargsyan Parandzem 
Published: August 2024

Purpose
This paper aims to analyze the factors that affect the attitudes of Armenian small and medium enterprises (SMEs) regarding digitalization, examining whether they view it as a tool to foster innovation and business growth, while also assessing the extent to which digitalization mitigated the negative impacts of the COVID-19 crisis.

Design/methodology/approach
The empirical approach is based on data collected from 452 Armenian SMEs, which were further analyzed using SPSS Statistics 23.0 software. Scales for assessing the COVID-19 impact, innovation and digitalization were constructed and validated. To test five hypotheses, binomial logistic regression was conducted to appraise the importance of digitalization for Armenian SMEs, including the ability to mitigate the consequences of the recent COVID-19 pandemic.

Findings
The results of the analysis showed that the economic sector plays a significant role in forming the expectations of firms on the positive impact of digitalization, while the company size does not affect them. This paper found that the provision of online services before the pandemic did not help Armenian SMEs to be less affected by the crisis. Furthermore, the perception of whether digitalization facilitated innovative processes, products and services did not depend on the degree of being affected by COVID-19. Finally, the findings indicate that the more agile companies which had the ability to convert their business model into an e-business model were less affected by the crisis.

Originality/value
This paper contributes to the literature by studying the effect of digitalization on SMEs, as well as how SMEs in small developing countries like Armenia mitigated the negative effects of the COVID-19 pandemic. The current work is unique as, to the best of the authors’ knowledge, there are no prior empirical studies focusing on impact of digitalization and innovation induced by exogenous shocks in an under-researched country like Armenia.
Impact of balcony geometry on the performance of rubberized concrete structures against progressive collapse
Authors: Alshaikh Ibrahim M.H. , Abadel Aref A. , Nehdi Moncef L. , Hamoda Ahmed 
Published: August 2024

PurposeEvaluate the performance of progressive collapse of full-scale three-dimensional structure (3D) beam-slab substructures with and without the presence of reinforced concrete (RC) balconies using two concrete mixes [normal concrete (NC) and rubberized concrete (RuC)].Design/methodology/approachThis study examines two concrete mixes to evaluate the progressive collapse performance of full-scale 3D beam-slab substructures with and without the presence of RC balconies using the finite element (FE) method.FindingsThe results showed that the vertical loads that affect the structures of the specimens after including the balconies in the modeling increased by an average of 29.3% compared with those of the specimens without balconies. The specimens with balconies exhibited higher resistance to progressive collapse in comparison with the specimens without balconies. Moreover, the RuC specimens performed very efficiently during the catenary stage, which significantly enhanced robustness to substantial deformation to delay or mitigate the progressive collapse risk.Originality/valueAll the experimental and numerical studies of the RC beam-slab substructures under progressive collapse scenarios are limited and do not consider the balcony’s presence in the building. Although balconies represent a common feature of multistory residential buildings, their presence in the building has more likely caused the failure of this building compared with a building without balconies. However, balconies are an external extension of RC slabs, which can provide extra resistance through tensile membrane action (TMA) or compressive membrane action (CMA). All those gaps have not been investigated yet.