bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive Assessment of Initial Adaptation of ESBL Positive ST131Escherichia colito Carbapenem Exposure
Authors: Shropshire William C. , Song Xinhao , Bremer Jordan , Seo Seokju , Rodriguez Susana , Anand Selvalakshmi Selvaraj , Dinh An Q. , Bhatti Micah M. , Konovalova Anna , Arias Cesar A. , Kalia Awdhesh , Shamoo Yousif , Shelburne Samuel A. 
Published: August 2024

ABSTRACTBackgroundIt remains unclear how high-riskEscherichia colilineages, like sequence type (ST) 131, initially adapt to carbapenem exposure in their progression to becoming carbapenem resistant.MethodsCarbapenem mutation frequency was measured in multiple subclades of extended-spectrum β-lactamase (ESBL) positive ST131 clinical isolates using a fluctuation assay followed by whole genome sequencing (WGS) characterization. Genomic, transcriptomic, and porin analyses of ST131 C2/H30Rx isolate, MB1860, under prolonged, increasing carbapenem exposure was performed using two distinct experimental evolutionary platforms to measure fast vs. slow adaptation.ResultsAll thirteen ESBL positive ST131 strains selected from a diverse (n=184) ST131 bacteremia cohort had detectable ertapenem (ETP) mutational frequencies with a statistically positive correlation between initial ESBL gene copy number and mutation frequency (r = 0.87,P-value <1e-5). WGS analysis of mutants showed initial response to ETP exposure resulted in significant increases in ESBL gene copy numbers or mutations in outer membrane porin (Omp) encoding genes in the absence of ESBL gene amplification with subclade specific associations. In both experimental evolutionary platforms, MB1860 responded to initial ETP exposure by increasingblaCTX-M-15copy numbers via modular, insertion sequence26(IS26) mediated pseudocompound transposons (PCTns). Transposase activity driven by PCTn upregulation was a conserved expression signal in both experimental evolutionary platforms. Stable mutations in Omp encoding genes were detected only after prolonged increasing carbapenem exposure consistent with clinical observations.ConclusionsESBL gene amplification is a conserved response to initial carbapenem exposure, especially within the high-risk ST131 C2/H30Rx subclade. Targeting such amplification could assist with mitigating carbapenem resistance development.
A simplein vitrofermentation model to detect alterations in gut microbiota-dependent bile acid profiles
Authors: Zheng Weijia , Bakker Wouter , Baccaro Marta , Jin Maojun , Wang Jing , Rietjens Ivonne M.C.M. 
Published: August 2024

AbstractA previousin vivostudy showed that lincosamide antibiotics (lincomycin and clindamycin) could induce changes in the gut bacterial community, leading to significant changes in fecal bile acid profiles. Herein, our aim is to develop an animal alternativein vitromodel for studying gut microbiota-dependent bile acid profiles induced by xenobiotics. The effects of lincosamides were evaluated using this model, and results obtained were verified by comparing with those of the previousin vivostudy. Fecal sample processing and bile acid incubation conditions were developed and optimized using feces collected from Wistar rats, and prepared samples were incubated for 24 h with or without lincosamides. Upon treatment of the fecal gut microbiota with lincosamides primary and secondary bile acids showed obviously increased and decreased levels respectively. Moreover, the changes in bile acid profile could be linked to a reduced richness of familyErysipelotrichaceae,BacteroidaceaandLactobacillaceaeorPrevotellaceae. The consistent consequences ofin vivoandin vitroprovides a proof of principle for further application on elucidating effects of other xenobiotics on the gut bacterial community and related bile acid metabolism, thereby contributing to the 3Rs (replacement, reduction and refinement) in animal testing.
CRISPR-Cas12a bends DNA to destabilize base pairs during target interrogation
Authors: Soczek Katarzyna M. , Cofsky Joshua C. , Tuck Owen T. , Shi Honglue , Doudna Jennifer A. 
Published: August 2024

ABSTRACTRNA-guided endonucleases are involved in processes ranging from adaptive immunity to site-specific transposition and have revolutionized genome editing. CRISPR-Cas9, -Cas12 and related proteins use guide RNAs to recognize ∼20-nucleotide target sites within genomic DNA by mechanisms that are not yet fully understood. We used structural and biochemical methods to assess early steps in DNA recognition by Cas12a protein-guide RNA complexes. We show here that Cas12a initiates DNA target recognition by bending DNA to induce transient nucleotide flipping that exposes nucleobases for DNA-RNA hybridization. Cryo-EM structural analysis of a trapped Cas12a-RNA-DNA surveillance complex and fluorescence-based conformational probing show that Cas12a-induced DNA helix destabilization enables target discovery and engagement. This mechanism of initial DNA interrogation resembles that of CRISPR-Cas9 despite distinct evolutionary origins and different RNA-DNA hybridization directionality of these enzyme families. Our findings support a model in which RNA-mediated DNA engineering begins with local helix distortion by transient CRISPR-Cas protein binding.
Orders of Magnitude Improved Cyclotron-Mode Cooling for Nondestructive Spin Quantum Transition Spectroscopy with Single Trapped Antiprotons
Authors: Latacz B. M. , Fleck M. , Jäger J. I. , Umbrazunas G. , Arndt B. P. , Erlewein S. R. , Wursten E. J. , Devlin J. A. , Micke P. , Abbass F. , Schweitzer D. , Wiesinger M. , Will C. , Yildiz H. , Blaum K. , Matsuda Y. , Mooser A. , Ospelkaus C. , Smorra C. , Soter A. , Quint W. , Walz J. , Yamazaki Y. , Ulmer S. ,   BASE Collaboration
Published: August 2024

We demonstrate efficient subthermal cooling of the modified cyclotron mode of a single trapped antiproton and reach particle temperatures T+=E+/kB below 200 mK in preparation times shorter than 500 s. This corresponds to the fastest resistive single-particle cyclotron cooling to subthermal temperatures ever demonstrated. By cooling trapped particles to such low energies, we demonstrate the detection of antiproton spin transitions with an error rate <0.000 023, more than 3 orders of magnitude better than in previous best experiments. This method has enormous impact on multi-Penning-trap experiments that measure magnetic moments with single nuclear spins for tests of matter and antimatter symmetry, high-precision mass spectrometry, and measurements of electron g factors bound to highly charged ions that test quantum electrodynamics and establish standards for magnetometry.

Published by the American Physical Society
2024
Does corporate environmental disclosure affect earnings management? The moderating effect of female board representation
Authors: Ardhaoui Roua , Ben Amar Anis , Fakhfakh Ines 
Published: August 2024

PurposeThis paper aims to investigate the effect of corporate environmental disclosure on earnings management and to further examine whether this relationship is moderated by female board.Design/methodology/approachOur sample includes 264 European companies listed on the STOXX eUROPE 600 for the period 2010 to 2022. We excluded financial companies (banks and insurance companies) due to their specific capital structure and regulatory requirements, and companies with missing data. Feasible Generalized Least Square (FGLS) regression method is used to estimate the econometric models. For robustness analyses, the authors included the alternative measure of the dependent variable, and they applied the simultaneous equation model for the endogeneity test.FindingsUsing discretionary accruals as a proxy for earnings management, the results obtained indicated a negative effect of corporate environmental disclosure on earnings management. The results suggest also that women on boards are effective in their monitoring role. Indeed, findings show that the effect of corporate environmental disclosure on earnings management is particularly stronger with the presence of women directors on the companies’ boards.Research limitations/implicationsThis study has two limitations. Firstly, the sample size is relatively small, which may limit the generalizability of our findings. Secondly, our earnings management indicator, based on estimates of accruals, may not perfectly reflect all streams of earnings management. Therefore, to reduce potential bias in these estimates, it would be useful to use other indicators, such as real earnings management.Practical implicationsThe findings have several implications for regulatory, investors and academic researchers. For regulators, it is appropriate to promote several standards related to corporate environmental disclosure and earnings management. The results advise also the worldwide policy maker to give the importance of female roles to improve engagement firms in corporate environmental disclosure, so to be more transparent in their accounting practices to ensure that they are not engaging in unethical or fraudulent behavior. For investors, the results show that the existence of female directors on the board reduces earnings management. For academic researchers, it is interesting to explore the relationship between corporate environmental disclosure, women on the board, and earnings management.Originality/valueThis paper extends the existing literature by examining the moderating effect of women directors on the relationship between corporate environmental disclosure and earnings management in the European context.
Mapping the literature on augmented reality-based gaming: a text mining approach
Authors: Borgohain Dhruba Jyoti , Verma Manoj Kumar 
Published: August 2024

Purpose
This study aims to focus on carrying out a quantitative analysis of publications related to augmented reality (AR)-based gaming. AR combines the real world with the digital world and is a large basis of data in an ICT-based digital society. Therefore, there is a need for a comprehensive bibliometric review of research papers on AR-based gaming to identify the potential for the advancement of knowledge in this field.

Design/methodology/approach
In the experimental part, reference data from Scopus was extracted according to a search string formulated with great care. The retrieved data were then analyzed through open-source, sophisticated bibliometric tools, VOSviewer, along with Biblioshiny-R.

Findings
After running the analysis, it turns out that the years with the biggest number of publications were in 2010 and 2012. The focus areas of study include mixed reality, surveys and helmet-mounted displays. The USA was the most connected country in the research collaboration network; it has strong links with other nations including the UK, Canada and Greece, which cluster around it in the map. This trend of monetarily increasing number of papers depicts the growing interest and sustainable productivity in this research domain.

Originality/value
The purpose of this paper is to present a novel perspective on AR-based gaming, which has not been so far examined by bibliometric analysis. The results provide important information for researchers and stakeholders. In addition to filling a void in the literature, this study is an important reference for those researchers who are interested in exploring evolution and trends in AR-based gaming research.
Construction economics at early stage: order of cost estimating, life cycle costing and development appraisal of a proposed office complex
Authors: Amadi Alolote I. 
Published: August 2024

PurposeThis study is carried out to demonstrate the computational practicalities of environmental construction economics necessary to offer early-stage cost advice. A case study of a private sector client’s development proposal is used. This is for the acquisition of a vacant freehold land of 1.2 acres brownfield site to develop a Grade A office complex with plans to achieve the BREEAM Excellent rating green building certification.Design/methodology/approachA three-stage methodology was deployed: Order of cost estimating, before life cycle costing and then development appraisal. The Order of Cost Estimate is generated using the BCIS online database, following the procedural guideline of the New Rules Measurement (NRM). The life cycle costing was carried out from an environmental perspective to explore two design options – Design A and Design B, in terms of which would offer the best value for money whilst reducing carbon emissions.FindingsBased on the outcome of the life cycle costing computations, Design B was chosen as the advised development due to minimal differences in net present values and annual equivalents. Further evaluation of Design B, using the residual method of developmental appraisal was carried out, with all necessary assumptions made. From the extensive computations carried out, the project is considered unviable, as it reports a loss. Alternative use of the site or an alternative site is thus recommended to check if a greater return on investment is tenable.Originality/valueThe study narratively interweaves the application of three computational techniques that are core to offering early-stage cost advice.
Innovative, smart, green and halal: a recipe for marketing Muslim destination
Authors: Suhartanto Dwi , Amalia Fatya Alty , Sugiama Aceng Gima , Choirisa Septi Fahmi , Nova Muhamad 
Published: August 2024

Purpose
This study explores tourist loyalty as a key for marketing Muslim destinations by integrating experience quality, innovation, smart technology and environmental concerns as determinants.

Design/methodology/approach
A quantitative research approach was applied by gathering data from 442 inbound Muslim tourists who visited various tourist destinations in Indonesia. PLS-SEM was utilized to examine the hypothesized association between the construct variables.

Findings
The data analysis reveals that the inclusion of smart technology and environmental concerns strengthens the innovation-quality-loyalty model. Specifically, this study highlights that smart tourism technology, and environmental concerns are essential to drive the quality of destination innovation and tourist holistic experience, which subsequently impact tourist loyalty.

Practical implications
This study provides hints to the marketing managers of Muslim tourist destinations to make their destination sustainable by creating loyalty among their Muslim visitors. In order to achieve this target, Muslim destinations are suggested to utilize smart technology and create environmentally friendly destinations to push innovation.

Originality/value
To the best of the authors’ knowledge, this is the first examination of the amalgamating innovation-quality-loyalty model with smart tourism technology and environmental concerns in the context of halal tourism.
Preschool teachers’ perspectives about diversity in nation-state country: a glimpse from Türkiye
Authors: Yigit Gencten Vahide , Gultekin Mehmet 
Published: August 2024

Purpose
This study aims to investigate preschool teachers' perspectives on diversity in the unique educational context of Türkiye, a nation-state often underrepresented in the predominantly Western-centric diversity literature.

Design/methodology/approach
Recognizing teachers as critical content integrators, the study involved semi-structured interviews to explore their views on diversity within early childhood education.

Findings
Analysis of the interviews revealed two key themes: first, the challenge of maintaining nation-state ideology, where teachers balance fostering a national identity with acknowledging diverse cultural backgrounds; and second, a willingness among educators to embrace diversity, highlighting their efforts to create inclusive, multicultural classrooms.

Practical implications
The authors call for developing context-specific teacher education programs addressing diversity in depth, equipping teachers to effectively promote context-specific multicultural education that would be different from Western countries.

Originality/value
To the best of the authors’ knowledge, this study is original in researching preschool teachers’ understandings of multicultural education in a nation-state country.
Effects of interactive-mobile learning modules in students’ engagement and understanding in genetics
Authors: Errabo Denis Dyvee , Ongoco Areeya Amor 
Published: August 2024

PurposeThe principal aim of the research was to develop Interactive Mobile Learning Modules (IMLM) as an approach to innovative teaching practices in online distance education.Design/methodology/approachThe study employed educational action research, using a quasi-experimental design catering to ninth-grade students (N = 104) attending a public high school in Manila.FindingsOur research indicates that IMLM has a positive effect on student engagement. It provides students with a convenient, easily accessible, and engaging means of understanding Genetics concepts. The execution of this initiative has facilitated the emergence of novel ideas, heightened the ease of use, and advanced more equitable opportunities within the education domain. Thus, IMLM’s utilization has resulted in a favorable shift in conceptual understanding. It has been found to support learners' concept test performance, as evidenced by score gains and statistically significant improvement in understanding.Research limitations/implicationsThe study is limited with the development and utilization of mlearning strategy to accommodate learners in the public school system in a developing country.Practical implicationsThe study addresses ongoing strategy and discussion to enrich online learning through the mlearning strategy.Social implicationsThe study accommodates inclusivity and equitable learning through personalize mlearning strategy.Originality/valueThe study is novel because it utilize mlearning as innovative approach to teaching genetics.
Ethics education in management in academia and HRD: an identity control theory-based model
Authors: Eyal Ori , Berkovich Izhak 
Published: August 2024

Purpose
The current body of research has separately examined ethics education design and evaluation, as well as the development of ethical identity in managers. However, a notable deficiency in the literature lies in the absence of a comprehensive investigation into the interconnections between these two areas. This conceptual paper aims to address this lacuna.

Design/methodology/approach
Building on the theoretical foundations of identity control theory, this paper presents a conceptual model that outlines the dynamics of ethics education for managers, whether outside the organization or as a human resource development (HRD) initiative. Drawing upon a diverse range of literature sources, the model places significant emphasis on the interactive nature of identity formation, taking into account both individual agency and the educational context.

Findings
The conceptual model developed based on identity control theory illuminates the functioning of ethics education and its impact. The model illustrates the multifaceted nature of the relationship between ethics education and the development and sustenance of ethical identity in managers. It underscores the iterative process of identity control, wherein managers continuously navigate their ethical identities in response to internal and external influences.

Originality/value
While ethics education in management and HRD studies is widely acknowledged, there is a significant gap in understanding the psychological mechanisms that explain the maintenance of self-identity and the dynamic interplay between individuals and their social environment. This gap is particularly relevant to educational programs, which not only shape the social environment for trainees but also aim to foster the development and preservation of their individual identities.
Shared use of a mass‐flowering crop drives dietary niche overlap between managed honeybees and bumblebees
Authors: Bernhardsson Olivia , Kendall Liam , Olsson Ola , Olsson Peter , Smith Henrik G. 
Published: August 2024

Abstract

Resource competition between wild pollinators and managed honeybees (Apis mellifera) has the potential to detrimentally impact insect biodiversity as well as wild plant and crop pollination. As honeybees are central place foragers, their competitive impact on wild bees is expected to be structured by hive proximity, in conjunction with foraging decisions related to landscape‐level resource availability. Yet, how these factors structure dietary niche overlap between wild bees and managed honeybees remains unclear.

We conducted a field experiment in seminatural grasslands, where honeybee foraging densities and niche overlap with buff‐tailed bumblebees (Bombus terrestris) were measured at four distances (<100, 500, 1000 and 2000 m) from experimentally placed apiaries, during and after the blooming period of mass‐flowering oilseed rape (Brassica napus). We measured honeybee foraging densities using standardized transect surveys, and quantified species diet composition and dietary niche overlap from pollen samples collected from both bee species.

Honeybee foraging densities were highest near apiaries and declined sharply beyond 500 m from the apiaries. However, niche overlap was unrelated to hive proximity but positively related to the availability of oilseed rape. Furthermore, there were significant inter‐ and intraspecific differences in pollen diet composition and breadth throughout the season.

Synthesis and applications: Niche overlap between honeybees and bumblebees in agricultural environments was due to resource sharing of mass‐flowering oilseed rape. When both honeybees and bumblebees predominantly forage on wild plants, they maintain distinct pollen diets, suggesting there is a low risk of resource competition between these generalist taxa. Conservation actions that promote floral resource availability and diversity in agricultural landscapes are crucial to maintain niche differentiation between managed honeybees and wild bees.
Fumonisin B1 induces endoplasmic reticulum damage and inflammation by activating the NXR response and disrupting the normal CYP450 system, leading to liver damage in juvenile quail
Authors: Zhu Lingxin , Li Jinhong , Yang Shuang , Deng Xiaoqi , Wang Zhenchao , Cao Changyu 
Published: August 2024

AbstractFumonisin B1 (FB1) is a mycotoxin affecting animal health through the food chain and has been closely associated with several diseases such as pulmonary edema in pigs and diarrhea in poultry. FB1 is mainly metabolized in the liver. Although a few studies have shown that FB1 causes liver damage, the molecular mechanism of liver damage is unclear. This study aimed to evaluate the role of liver damage, nuclear xenobiotic receptor (NXR) response and cytochrome P450 (CYP450)‐mediated defense response during FB1 exposure. A total of 120 young quails were equally divided into two groups (control and FB1 groups). The quails in the control group were fed on a normal diet, while those in the FB1 group were fed on a quail diet containing 30 mg/kg for 42 days. Histopathological and ultrastructural changes in the liver, biochemical parameters, inflammatory factors, endoplasmic reticulum (ER) factors, NXR response and CYP450 cluster system and other related genes were examined at 14 days, 28 days and 42 days. The results showed that FB1 exposure impaired the metabolic function and caused liver injury. FB1 caused ER stress and decreased adenosine triphosphatease activity, induced the expression of inflammation‐related genes such as interleukin 6 and nuclear factor kappa‐B, and promoted inflammation. In addition, FB1 disrupted the expression of multiple CYP450 isoforms by activating nuclear xenobiotic receptors (NXRs). The present study confirms that FB1 exposure disturbs the homeostasis of cytochrome P450 systems (CYP450s) in quail liver by activating NXR responses and thereby causing liver damage. This study's findings provide insight into the molecular mechanisms of FB1‐induced hepatotoxicity.