bluuebunny/crossref_metadata_2025_split huggingface dataset:
Benchmarking Performance Indices of Electrochemical CO2 Reduction to Ethylene Based on Prospective Life Cycle Assessment for Negative Emissions
Authors: Yamaguchi Shingi , Amasawa Eri , Ebe Hiroji , Hirao Masahiko , Sugiyama Masakazu 
Published: August 2024

AbstractTo mitigate global warming to the most ambitious targets, it is necessary to remove CO2 from the atmosphere and reduce fossil fuels use. The electrochemical conversion of CO2 to ethylene (C2H4) as a basic chemical is a promising technology that meets both requirements; however, its life cycle CO2 emissions remain inconclusive because of varying assumptions in the performance indices. This study aimed to set benchmarks for the four most sensitive indices to achieve −0.5 t‐CO2/t‐C2H4 by calculating net greenhouse gas (GHG) emissions through a prospective life cycle assessment of a model system including CO2 capture, CO2 enrichment, electrochemical conversion, CO2 recycling, and cryogenic separation. As a result, the electrochemical conversion process was the hotspot of life cycle emissions, and representative benchmarks were determined as follows: cell voltage, 3.5 V; C2H4 Faraday efficiency, 70 %; conversion rate, 20 %; and electrochemical CO2 recycling energy, 2.2 GJ/t‐CO2. The gaps between the benchmarks and current top data of cell voltage and Faraday efficiency were <10 %, and suppressing the performance degradation for up to one year was found to be a critical requirement. These results can direct research towards the development of a year‐round stable system, rather than further improving the performance indices.
A Fractional‐Order Method of Frequency Splitting and Bifurcation Suppression for Wireless Power Transfer Systems
Authors: Shu Xujian , Zhang Xueqi , Jiang Yanwei , Zhang Bo 
Published: August 2024

ABSTRACTWireless power transfer (WPT) is an emerging technology that enables the wireless transfer of electrical energy from power supplies to electrical equipment. It has been widely used in electric vehicles, mobile phones, household appliances, medical devices, and other fields. However, the frequency splitting and bifurcation phenomena existing in WPT systems are the fundamental obstacles and challenges that affect the effective operation of WPT systems. In this paper, a method based on the fractional‐order circuit is proposed to simultaneously suppress the frequency splitting and bifurcation phenomena by changing the order of fractional‐order capacitor. By replacing the compensation capacitor in the transmitter of the traditional WPT system with a fractional‐order capacitor, a fractional‐order WPT system is formed. Then, using fractional calculus and circuit theory, the mathematical model of the proposed WPT system containing a fractional‐order capacitor is established, and the frequency splitting and bifurcation phenomena are analyzed. The theoretical results show that the frequency splitting and bifurcation phenomena are suppressed only by adjusting the order of fractional‐order capacitor, and the output power of the original resonant frequency is improved. Finally, the experimental prototype is implemented to validate the theoretical results.
Single‐cell multi‐modal chromatin profiles revealing epigenetic regulations of cells in hepatocellular carcinoma
Authors: Wang Chunqing , Huang Waidong , Zhong Yu , Zou Xuanxuan , Liu Shang , Li Jie , Sun Yunfan , Zhou Kaiqian , Chen Xi , Li Zihao , Wang Shanshan , Huang Yaling , Bai Yinqi , Yin Jianhua , Jin Xin , Liu Shiping , Yuan Yue , Deng Qiuting , Jiang Miaomiao , Liu Chuanyu , Liu Longqi , Xu Xun , Wu Liang 
Published: August 2024

AbstractBackgroundVarious epigenetic regulations systematically govern gene expression in cells involving various biological processes. Dysregulation of the epigenome leads to aberrant transcriptional programs and subsequently results in diseases, such as cancer. Therefore, comprehensive profiling epigenomics is essential for exploring the mechanisms underlying gene expression regulation during development and disease.MethodsIn this study, we developed single‐cell chromatin proteins and accessibility tagmentation (scCPA‐Tag), a multi‐modal single‐cell epigenetic profile capturing technique based on barcoded Tn5 transposases and a droplet microfluidics platform. scCPA‐Tag enables the simultaneous capture of DNA profiles of histone modification and chromatin accessibility in the same cell.ResultsBy applying scCPA‐Tag to K562 cells and a hepatocellular carcinoma (HCC) sample, we found that the silence of several chromatin‐accessible genes can be attributed to lysine‐27‐trimethylation of the histone H3 tail (H3K27me3) modification. We characterized the epigenetic features of the tumour cells and different immune cell types in the HCC tumour tissue by scCPA‐Tag. Besides, a tumour cell subtype (C2) with more aggressive features was identified and characterized by high chromatin accessibility and a lower abundance of H3K27me3 on tumour‐promoting genes.ConclusionsOur multi‐modal scCPA‐Tag provides a comprehensive approach for exploring the epigenetic landscapes of heterogeneous cell types and revealing the mechanisms of gene expression regulation during developmental and pathological processes at the single‐cell level.Highlights
scCPA‐Tag offers a highly efficient and high throughput technique to simultaneously profile histone modification and chromatin accessibility within a single cell.
scCPA‐Tag enables to uncover multiple epigenetic modification features of cellular compositions within tumor tissues.
scCPA‐Tag facilitates the exploration of the epigenetic landscapes of heterogeneous cell types and provides the mechanisms governing gene expression regulation.
Ecological and evolutionary dynamics to design and improve ovarian cancer treatment
Authors: Han Grace Y. Q. , Alexander Monica , Gattozzi Julia , Day Marilyn , Kirsch Elayna , Tafreshi Narges , Chalar Raafat , Rahni Soraya , Gossner Gabrielle , Burke William , Damaghi Mehdi 
Published: August 2024

AbstractOvarian cancer ecosystems are exceedingly complex, consisting of a high heterogeneity of cancer cells. Development of drugs such as poly ADP‐ribose polymerase (PARP) inhibitors, targeted therapies and immunotherapies offer more options for sequential or combined treatments. Nevertheless, mortality in metastatic ovarian cancer patients remains high because cancer cells consistently develop resistance to single and combination therapies, urging a need for treatment designs that target the evolvability of cancer cells. The evolutionary dynamics that lead to resistance emerge from the complex tumour microenvironment, the heterogeneous populations, and the individual cancer cell's plasticity. We propose that successful management of ovarian cancer requires consideration of the ecological and evolutionary dynamics of the disease. Here, we review current options and challenges in ovarian cancer treatment and discuss principles of tumour evolution. We conclude by proposing evolutionarily designed strategies for ovarian cancer, with the goal of integrating such principles with longitudinal, quantitative data to improve the treatment design and management of drug resistance.Key points/Highlights
Tumours are ecosystems in which cancer and non‐cancer cells interact and evolve in complex and dynamic ways.
Conventional therapies for ovarian cancer inevitably lead to the development of resistance because they fail to consider tumours’ heterogeneity and cellular plasticity.
Eco‐evolutionarily designed therapies should consider cancer cell plasticity and patient‐specific characteristics to improve clinical outcome and prevent relapse.
Urbanization alters the relative importance of local and landscape factors affecting plant communities in the Tokyo megacity
Authors: Iwachido Yuki , Uchida Kei , Sasaki Takehiro 
Published: August 2024

AbstractPlant communities are impacted by local factors (related to environmental filtering) and landscape factors (related to dispersal limitation). While many studies have shown that the relative importance of these factors in understanding plant community dynamics due to urbanization, little is known about how they are altered by urbanization—a significant threat to biodiversity. This study evaluates the relative importance of local environmental (local factors), landscape, and spatial (landscape factors) variables that influence plant communities in 34 urban green spaces comprising two different habitats (forests and grasslands) along the urban–rural gradients in the Tokyo megacity, Japan. To continuously assess the relative importance of each factor along the urban–rural gradients, we extracted 1000 landscapes within a certain range that contained several sites. Subsequently, the relative importance of each factor and urbanization rate (proportion of artificial built‐up area) were estimated for each landscape. Our study found that the relative importance of both local and landscape factors decreased, while that of local factor for native species in forest habitats and that of landscape factors for native species in grassland habitats increased. Collectively, these findings suggest that city size and habitat characteristics must be considered when predicting changes in plant communities caused by urbanization.
Brooding duration does not depend on cat predation risk but is related to weather and phenology in the wandering albatross (Diomedea exulans)
Authors: Bourgoin Charlotte , Barbraud Christophe , Getti Tobie , Delord Karine , Angelier Frédéric , Bodin Aymeric , Blanchard Pierrick 
Published: August 2024

AbstractParental investment increases offspring fitness at the expense of the parent's ability to invest in other offspring. In many animal species, parents guard their offspring after birth. The parental decision over the duration of this period is expected to be triggered by the associated fitness costs and benefits for both offspring and parents. Here, we evaluated the relevance of several intrinsic and environmental variables in determining brooding period duration in the wandering albatross (Diomedea exulans) and questioned whether brooding duration was related to chick subsequent survival and biometry prior to fledging. We used a semi‐experimental design to increase the variance in cat abundance, a recent predator of albatross chicks, and predicted that an increased predation risk at the nest scale would trigger longer chick brooding and thus, protection. In addition, we questioned the influence of weather conditions, hatching date, and characteristics of chicks (sex and biometry) and parents (sex and age) on brooding duration. We report no effect of predation risk or parental characteristics on brooding duration. However, the probability for a parent to end brooding decreased with forthcoming unfavorable weather. Our data also revealed reduced brooding duration for late‐hatched chicks and a positive association between brooding duration and chick structural size, and between the frequency of shifts between parents and chick structural size. Finally, brooding duration was not associated with chick survival or with chick biometry prior to fledging. We discuss these results in light of pre‐existing hypotheses on fitness costs and benefits associated with brooding duration for chicks and parents.
Seasonal changes in invertebrate diet of breeding black‐necked cranes (Grus nigricollis)
Authors: Ma Ruifeng , Ma Shujuan , Liu Hongyi , Hu Lei , Li Yudong , He Ke , Zhu Ying 
Published: August 2024

AbstractInvertebrates greatly support the growth, development, and reproduction of insectivorous birds. However, the influence of human activity (e.g., pesticide use, deforestation, and urbanization) inevitably leads to a decrease in global arthropods. The diversity and variation in invertebrate diet influence the food composition of birds, especially species living in rapidly changing environments, such as the Tibetan Plateau. However, little is known of the seasonal variation in invertebrate diet in response to environmental changes. Here, we characterized the invertebrate diet composition in pre‐ and post‐breeding black‐necked crane (Grus nigricollis) using fecal metabarcoding. We identified 38 invertebrate genera; the top three were Tipula (82.1% of relative abundance), Ceramica (3.0%), and unclassified_Hymenoptera (2.5%), with Tipula predominated the diet in both seasons. We also observed 20 and 16 unique genera in the pre‐ and post‐breeding periods, and the genera composition was distinct between seasons (R = .036, p = .024). In pre‐breeding, black‐necked cranes tended to consume more diverse foods, and individual cranes exhibited greater heterogeneity at the genus level. At the genera and species level, pre‐breeding black‐necked cranes showed a wider dietary niche than post‐breeding cranes. We observed season‐specific features, with Tipula (common crane fly) and Stethophyma (grasshoppers) being enriched in the post‐breeding period and Ceramica (moth) being more abundant in the pre‐breeding period. Three Tipula species had the greatest importance in discriminating between seasonal diets. This study demonstrated a seasonal pattern of invertebrate diet in the black‐necked crane, suggesting diet composition in response to resource and species availability. These results elaborate on the foraging ecology of highland birds and can inform the management of black‐necked crane conservation.
Prevalence and characteristics of transthyretin amyloid cardiomyopathy in hypertrophic cardiomyopathy
Authors: Garcia‐Pavia Pablo , Damy Thibaud , Piriou Nicolas , Barriales‐Villa Roberto , Cappelli Francesco , Bahus Catherine , Munteanu Carmen , Keohane Denis , Mallaina Pablo , Elliott Perry ,   the TTRACK investigators
Published: August 2024

AbstractAimsRecognition of transthyretin amyloid cardiomyopathy is increasing due to advances in cardiac imaging and diagnostic strategies, but questions remain regarding disease frequency and characteristics. We examined the prevalence and characteristics of transthyretin amyloid cardiomyopathy in older patients with hypertrophic cardiomyopathy of unascertained aetiology.Methods and resultsTTRACK was a multicentre, non‐interventional, cross‐sectional epidemiologic study funded by Pfizer and conducted in 20 hospitals and medical centres in 11 countries (NCT03842163). Eligible patients were aged ≥50 years, had hypertrophic cardiomyopathy (maximal end‐diastolic left ventricular wall thickness ≥15 mm on echocardiogram) without an identified genetic or alternative origin at study enrolment, and underwent 99mTechnetium bone scintigraphy, with or without single photon emission computed tomography (SPECT). Cardiac‐versus‐bone uptake on scans was visually scored from 0 to 3 (Perugini scoring). Patients with grades 1–3 underwent monoclonal protein and laboratory testing and transthyretin (TTR) gene sequencing. Of 766 eligible patients, 691 (90.2%) had scintigraphy alone and 75 (9.8%) scintigraphy plus SPECT. Two hundred and eight patients (27.2%) had grade 2 or 3 cardiac uptake on scintigraphy; 144 (18.8%) had grade 2 or 3 cardiac uptake and no evidence of plasma cell dyscrasia and were diagnosed with transthyretin amyloid cardiomyopathy. Of patients with transthyretin amyloid cardiomyopathy, 11 (7.6%) had a pathogenic TTR gene variant and 34 (23.8%), 74 (51.7%), and 35 (24.5%) had New York Heart Association class I, II, and III/IV heart failure (HF) symptoms, respectively. Clinical and laboratory diagnostic characteristics were observed in ≥90% of patients with transthyretin amyloid cardiomyopathy. The characteristics most strongly associated with transthyretin amyloid cardiomyopathy on multivariable analysis were carpal tunnel syndrome (odds ratio [OR] 54.3; P < 0.0001) and male sex (OR 7.9; P < 0.0001).ConclusionsIn the TTRACK study, almost one in five patients ≥50 years of age with hypertrophic cardiomyopathy had transthyretin amyloid cardiomyopathy. Greater awareness of the frequency and characteristics of transthyretin amyloid cardiomyopathy in older patients with hypertrophic cardiomyopathy are needed to help improve early detection of this debilitating but treatable disease.
Efficacy and safety of low‐dose digoxin in patients with heart failure. Rationale and design of the DECISION trial
Authors: van Veldhuisen Dirk J. , Rienstra Michiel , Mosterd Arend , Alings A. Marco , van Asselt Antoinette D.J. , Bouvy Marcel L , Tijssen Jan G.P. , Schaap Jeroen , van der Wall Ernst E. , Voors Adriaan A. , Boorsma Eva M. , Lok Dirk J.A. , Crijns Harry J.G.M. , Schut Astrid , Vijver Marlene A.T. , Voordes Geert H.D. , de Vos Agaath H. , Maas‐Soer Ester L. , Smit Nicoline W. , Touw Daan J. , Samuel Michelle , van der Meer Peter ,   DECISION Investigators and Committees
Published: August 2024

AbstractAimsDigoxin is the oldest drug in cardiovascular (CV) medicine, and one trial conducted >25 years ago showed a reduction in heart failure (HF) hospitalizations but no effect on mortality. However, later studies suggested that the dose of digoxin used in that trial (and other studies) may have been too high. The DECISION (Digoxin Evaluation in Chronic heart failure: Investigational Study In Outpatients in the Netherlands) trial will examine the efficacy and safety of low‐dose digoxin in HF patients with reduced or mildly reduced left ventricular ejection fraction (LVEF) with a background of contemporary HF treatment.MethodsThe DECISION trial is a randomized, double‐blind, parallel‐group, placebo‐controlled event‐driven outcome trial which will investigate the efficacy and safety of low‐dose digoxin in patients with chronic HF and LVEF <50%. Both patients with sinus rhythm and atrial fibrillation will be enrolled and will be randomized (1:1) to low‐dose digoxin or matching placebo. To maintain a target serum digoxin concentration of 0.5–0.9 ng/ml, dose adjustments are made throughout follow‐up based on serum digoxin measurements with dummy values for the placebo group. The primary endpoint is a composite of CV mortality and total HF hospitalizations or total urgent hospital visits for worsening HF, and all endpoints are adjudicated blindly by a Clinical Event Committee. The estimated sample size was 982 patients who will be followed for a median of 3 years, and in December 2023 enrolment was completed after 1002 patients.ConclusionsThe DECISION trial will provide important evidence regarding the effect of (low‐dose) digoxin on CV mortality and total HF hospitalizations and urgent hospital visits when added to contemporary HF treatment of patients with reduced or mildly reduced LVEF.Clinical Trial Registration: ClinicalTrials.gov identifier: NCT03783429.
Burst steroid therapy for acute heart failure: The CORTAHF randomized, open‐label, pilot trial
Authors: Cotter Gad , Davison Beth A. , Freund Yonathan , Voors Adriaan A. , Edwards Christopher , Novosadova Maria , Takagi Koji , Hayrapetyan Hamlet , Mshetsyan Andranik , Mayranush Drambyan , Cohen‐Solal Alain , ter Maaten Jozine M. , Biegus Jan , Ponikowski Piotr , Filippatos Gerasimos , Chioncel Ovidiu , Sadoune Malha , Pagnesi Matteo , Simon Tabassome , Metra Marco , Mann Douglas L. , Mebazaa Alexandre 
Published: August 2024

ABSTRACTAimsBurst steroid therapy, effective in acute respiratory diseases, may benefit patients with acute heart failure (AHF) in whom inflammatory activation is associated with adverse outcomes.Methods and resultsCORTAHF assessed whether burst steroid therapy reduces inflammation and results in better quality of life and clinical outcomes in AHF. Patients with AHF, N‐terminal pro‐B‐type natriuretic peptide >1500 pg/ml, and high‐sensitivity C‐reactive protein (hsCRP) >20 mg/L were randomized 1:1 to oral, once daily 40 mg prednisone for 7 days or usual care, without blinding. Patients were followed for 90 days. A total of 101 patients were randomized. At day 7 the primary endpoint, hsCRP decreased in both arms – adjusted geometric mean ratios (GMRs) were 0.30 and 0.40 in the prednisone and usual care arms (ratio of GMRs 0.75, 95% confidence interval [CI] 0.56–1.00, p = 0.0498). The 90‐day risk of worsening heart failure (HF), HF readmission or death as reported by the unblinded investigators was significantly lower in the prednisone group (10.4%) than in usual care (30.8%) (hazard ratio 0.31, 95% CI 0.11–0.86, p = 0.016). The EQ‐5D visual analogue scale score as reported by the unblinded patients increased more in the prednisone group on day 7 (least squares mean difference 2.57, 95% CI 0.12–5.01 points, p = 0.040). All effects were statistically significant in the pre‐specified subgroup with centrally‐measured interleukin‐6 >13 pg/ml. Adverse events, particularly hyperglycaemia, occurred more in the prednisone group with no difference in infection rate.ConclusionIn this small open‐label study of patients with AHF, burst steroid therapy was associated with reduced inflammation as measured by hsCRP levels at day 7 (primary endpoint). Secondary endpoints showed improved quality of life at day 7 and reduced 90‐day risk of death or worsening HF. Large prospective studies are needed to evaluate these findings.
Pyrene‐Functionalized Silicon Hybrid Porous Polymer for an Efficient Adsorption of Dyes
Authors: Yang Nan , Kunthom Rungthip , Liu Hongzhi 
Published: August 2024

AbstractPyrene‐functionalized silicon hybrid porous polymer (TAPPy‐HPP). The hybrid polymer was successfully prepared via a Schiff base reaction using tetra(4‐aminophenyl)pyrene (TAPPy) and tetra(4‐formylphenyl)silane (TFS) as the building blocks.. This hybrid polymer was characterized using IR, solid‐state 13C and 29Si NMR, and elemental analysis. Its morphology was analyzed by PXRD and SEM. Thermal analysis using Thermal analysis using TGA shows that the synthetic polymer has greater thermal stability than its monomers at a temperature of 5 % weight loss (Td5 %) of 392 °C. Although TAPPy‐HPP has a Brunauer‐Emmet‐Teller surface area of 45.4 m2 g−1, it adsorbed dyes with high efficiency, particularly the anionic dye Congo Red (CR), with an adsorption capacity of up to 358 m2 g−1. Moreover, TAPPy‐HPP showed excellent recycling and regenerative properties. This polymer is a promising candidate for a fluorescent chemical sensor for the absorption of dyes due to its exceptional physiochemical stability and intense luminescence.