bluuebunny/crossref_metadata_2025_split huggingface dataset:
An AP2/ERF member LlERF012 confers thermotolerance via activation of HSF pathway in lily
Authors: Li Ting , Wu Ze , Zhang Yinyi , Xu Sujuan , Xiang Jun , Ding Liping , Teng Nianjun 
Published: July 2024

AbstractHeat stress transcription factors (HSFs) are core factors of plants in response to heat stress (HS), but their regulatory network is complicated and remains elusive in a large part, especially HSFBs. In this study, we reported that the LlERF012‐LlHSFA1 module participates in heat stress response (HSR) by directly regulating HSF pathway in lily (Lilium longiflorum). LlHSFB1 was confirmed as a positive regulator in lily thermotolerance and a heat‐inducible AP2/ERF member LlERF012 (Ethylene Response Factor 012) was further identified to be a direct trans‐activator of LlHSFB1. Overexpression of LlERF012 elevated the thermotolerance of transgenic Arabidopsis and lily, but silencing LlERF012 reduced thermotolerance in lily. Further analysis showed LlERF012 interacted with LlHSFA1, which led to enhanced transactivation activity and DNA‐binding capability of LlERF012. In addition, LlERF012 also directly activated the expression of LlHSFA1 by binding its promoter. As expected, we found that LlERF012 bound the promoters of LlHSFA2, LlHSFA3A, and LlHSFA3B to stimulate their expression, and LlERF012‐LlHSFA1 interaction enhanced these activation effects. Overall, our data suggested that LlERF012 was a key factor for lily thermotolerance and the LlERF012‐LlHSFA1 interaction synergistically regulated the activity of the HSF pathway including the class A and B members, which might be of great significance for coordinating the functions of different HSFs.
The classification of melanocytic gene signatures
Authors: Hu Min , Coleman Samuel , Judson‐Torres Robert L. , Tan Aik Choon 
Published: July 2024

AbstractGene expression profiling technologies have revolutionized cell biology, enabling researchers to identify gene signatures linked to various biological attributes of melanomas, such as pigmentation status, differentiation state, proliferative versus invasive capacity, and disease progression. Although the discovery of gene signatures has significantly enhanced our understanding of melanocytic phenotypes, reconciling the numerous signatures reported across independent studies and different profiling platforms remains a challenge. Current methods for classifying melanocytic gene signatures depend on exact gene overlap and comparison with unstandardized baseline transcriptomes. In this study, we aimed to categorize published gene signatures into clusters based on their similar patterns of expression across clinical cutaneous melanoma specimens. We analyzed nearly 800 melanoma samples from six gene expression repositories and developed a classification framework for gene signatures that is resilient against biases in gene identification across profiling platforms and inconsistencies in baseline standards. Using 39 frequently cited published gene signatures, our analysis revealed seven principal classes of gene signatures that correlate with previously identified phenotypes: Differentiated, Mitotic/MYC, AXL, Amelanotic, Neuro, Hypometabolic, and Invasive. Each class is consistent with the phenotypes that the constituent gene signatures represent, and our classification method does not rely on overlapping genes between signatures. To facilitate broader application, we created WIMMS (what is my melanocytic signature, available at https://wimms.tanlab.org/), a user‐friendly web application. WIMMS allows users to categorize any gene signature, determining its relationship to predominantly cited signatures and its representation within the seven principal classes.
The relationship between the frequency of use of non‐medication coping strategies with urinary incontinence and depression in older adults
Authors: Öz Yildirim Özge , İŞeri Özge 
Published: July 2024

AbstractBackgroundThe aim of this study is to examine the relationship between the frequency of use of non‐medication coping strategies with urinary incontinence (UI) and depression in older adults.MethodsThis study followed a descriptive design and was conducted with 345 older adults over the age of 65. The data of the study were collected using the Geriatric Depression Scale Short Form and the Frequency of Non‐Medication Coping Strategies with Urinary Incontinence Scale. The data analysis of the study was conducted using descriptive statistics, Pearson correlation analysis, and hierarchical multiple regression analysis. The significance level was accepted as P < 0.05.ResultsThe mean age of the participants was 71.86 ± 5.43 years. Among the older adults, 58.8% were male and 50.2% were primary school graduates. It was determined that 43.8% of the participants experienced the frequency of UI more than once a week, 36.2% experienced stress incontinence, 51.9% consulted a physician due to UI, 53.6% received treatment for UI, and 47.8% reported that UI had an impact on their social life. The relationship between the frequency of use of non‐medication coping strategies with UI and geriatric depression (β = −0.017; P = 0.043) was found to be significant and negative. It was found that there was a significant and positive relationship between age, frequency of UI, type of UI, the impact of UI on social life, and geriatric depression (P < 0.05).Conclusion and SuggestionsThe older adults in this study were found to have moderate depression. It was determined that the level of depression was higher in the older adults who experienced UI more than once a week, those who experienced stress UI, those whose social lives were impacted, and those who had a higher frequency of non‐medication coping strategies.
The Historical Pronunciation of Koinē Greek and Why It MattersThe Pronunciation of New Testament Greek: Judeo‐Palestinian Greek Phonology and Orthography From Alexander to Islam, BenjaminKantor, Eerdmans, 2023 (ISBN 978‐0‐8028‐7831‐1), liv + 842 pp., hb $99.99A Short Guide to the Pronunciation of New Testament Greek, BenjaminKantor, Eerdmans, 2023 (ISBN 978‐0‐8028‐7832‐8), xvi + 134 pp., pb $14.99
Authors: Lookadoo Jonathon 
Published: July 2024

AbstractThis essay reviews two recent publications in historical Greek linguistics. While many in university and seminary education learn to read Greek texts, little attention is given to pronunciation and even less to how the language may have been pronounced historically. The volumes reviewed in this essay reconstruct changes in the pronunciation of Greek in Judeo‐Palestine by examining discrepancies in spelling and transliteration. The books also have implications for sociological studies in addition to pedagogical applications.
Epidermal growth factor receptor and programmed cell death‐1 expression levels in peripheral T cell subsets of patients with non‐small cell lung cancer
Authors: Ceylan Ayca , Artac Mehmet , Kocak Mehmet Zahid , Artac Hasibe 
Published: July 2024

AbstractLung cancer is the leading cause of cancer‐related deaths, in part due to its late diagnosis. Increased epidermal growth factor receptor (EGFR) expression in cancer cells is associated with a poor prognosis, and EGFR tyrosine kinase inhibitors are widely used in cancer treatment. This study aimed to clarify the relationship between EGFR expression on T cells and cancer prognosis in patients with non‐small cell lung cancer (NSCLC). Forty patients with NSCLC and 40 healthy volunteers were included in this study. Peripheral CD4+T helper (Th1, Th2, Th9, Th17, Th1Th17, follicular and peripheral Th) and cytotoxic T lymphocyte (CD8+follicular and peripheral T) subsets were identified with flow cytometry according to their chemokine receptors. EGFR expression on T lymphocytes in relation to overall survival (OS) was investigated in patients with NSCLC. The patients [mean age (min‐max) = 64.03 (45–83); 20 stage I–III and 20 stage IV] had increased EGFR expression on CD3+T, CD4+Th, Th1, Th2, and Th17 cells compared to the controls (p < 0.05). High EGFR expression on CD3+T, CD4+Th, Th1, and Th2 cells was associated with poor OS. Also, PD‐1 expression on lymphocytes, CD3+T, and Th cells was increased in patients with NSCLC compared to controls. The high expression of EGFR and PD‐1 on Th cells and the reduced percentage of lymphocytes and Th cells, especially in stage IV patients with NSCLC, revealed that increased EGFR activity may trigger apoptosis of Th cells and promote the development of metastases, while high EGFR expression on CD3+T, CD4+Th, Th1, and Th2 cells may be an independent poor prognostic marker in NSCLC.
Amniotic miracle: Investigating the unique development and applications of amniotic membrane in wound healing
Authors: Guo Xinya , Zhang Wei , Lu Jianyu , Zhu Yushu , Sun Hanlin , Xu Dayuan , Xian Shuyuan , Yao Yuntao , Qian Weijin , Lu Bingnan , Shi Jiaying , Ding Xiaoyi , Li Yixu , Tong Xirui , Xiao Shichu , Huang Runzhi , Ji Shizhao 
Published: July 2024

AbstractBackgroundThe perfect repair of damaged skin has always been a constant goal for scientists; however, the repair and reconstruction of skin is still a major problem and challenge in injury and burns medicine. Human amniotic membrane (hAM), with its good mechanical properties and anti‐inflammatory, antioxidant and antimicrobial benefits, containing growth factors that promote wound healing, has evolved over the last few decades from simple skin sheets to high‐tech dressings, such as being made into nanocomposites, hydrogels, powders, and electrostatically spun scaffolds. This paper aims to explore the historical development, applications, trends, and research hotspots of hAM in wound healing.MethodsWe examined 2660 publications indexed in the Web of Science Core Collection (WoSCC) from January 1, 1975 to July 12, 2023. Utilizing bibliometric methods, we employed VOSviewer, CiteSpace, and R‐bibliometrix to characterize general information, identify development trends, and highlight research hotspots. Subsequently, we identified a collection of high‐quality English articles focusing on the roles of human amniotic epithelial stem cells (hAESCs), human amniotic mesenchymal stem cells (hAMSCs), and amniotic membrane (AM) scaffolds in regenerative medicine and tissue engineering.ResultsBibliometric analysis identified Udice–French Research Universities as the most productive affiliation and Tseng S.C.G. as the most prolific author. Keyword analysis, historical direct quotations network, and thematic analysis helped us review the historical and major themes in this field. Our examination included the knowledge structure, global status, trends, and research hotspots regarding the application of hAM in wound healing. Our findings indicate that contemporary research emphasizes the preparation and application of products derived from hAM. Notably, both hAM and the cells isolated from it – hADSCs and hAESCs are prominent and promising areas of research in regenerative medicine and tissue engineering.ConclusionThis research delivers a comprehensive understanding of the knowledge frameworks, global dynamics, emerging patterns, and primary research foci in the realm of hAM applications for wound healing. The field is rapidly evolving, and our findings offer valuable insights for researchers. Future research outcomes are anticipated to be applied in clinical practice, enhancing methods for disease prevention, diagnosis, and treatment.
PbGBF3 enhances salt response in pear by upregulating PbAPL2 and PbSDH1 and reducing ABA‐mediated salt sensitivity
Authors: Dong Huizhen , Chen Qiming , Fu Yifei , Xie Haoyang , Li Tinghan , Li Dingli , Yang Yingjie , Xie Zhihua , Qi Kaijie , Zhang Shaoling , Huang Xiaosan 
Published: July 2024

SUMMARYPear is a widely cultivated fruit crop, but its distribution and sustainable production are significantly limited by salt stress. This study used RNA‐Seq time‐course analysis, WGCNA, and functional enrichment analysis to uncover the molecular mechanisms underlying salt stress tolerance in Pyrus ussuriensis. We identified an ABA‐related regulatory module, PbGBF3‐PbAPL2‐PbSDH1, as crucial in this response. PbGBF3, a bZIP transcription factor, enhances salt tolerance by upregulating PbAPL2 and PbSDH1. Overexpression of PbGBF3 improved salt tolerance in Pyrus communis calli and Arabidopsis, while silencing it reduced tolerance in Pyrus betulifolia. Functional assays showed that PbGBF3 binds to the promoters of PbAPL2 and PbSDH1, increasing their expression. PbAPL2 and PbSDH1, key enzymes in starch synthesis and the sorbitol pathway, respectively, enhance salt tolerance by increasing AGPase activity, soluble sugar content, and SDH activity, improving ROS scavenging and ion balance. Our findings suggest that the PbGBF3‐PbAPL2 and PbGBF3‐PbSDH1 modules positively regulate salt tolerance by enhancing ABA signaling and reducing ABA‐mediated growth inhibition. These insights provide a foundation for developing salt‐tolerant pear cultivars.
The ‘Coloured’ connection of Namibian English
Authors: Stell Gerald 
Published: July 2024

AbstractAmong the earliest Afrikaans‐English bilinguals in Namibia, South African Coloureds formed an essential part of an ethnically fluid intermediate social class during the country's colonial period. Using phonetic and perceptual data from a young multi‐ethnic urban sample, this study investigates the contribution of Coloured English to Namibian English. The study finds that, in terms of vowel realization patterns, Coloured Namibian English varieties sit in between White and Black Namibian English varieties, forming part of a continuum in which one extremity tends towards White South African English. The less ‘Afrikaans‐accented’ Coloured English varieties—associated with women—are developing middle‐class indexicalities and are targeted by young Black women. Quantitative and qualitative perceptual data show that these varieties enjoy high status across Namibia's ethnolinguistic spectrum. The study concludes that Namibian English is norm‐developing, as befits any Outer Circle variety, and that Nambian Coloureds exert a prominent normative influence on it.
Avalanche burial pathophysiology – a unique combination of hypoxia, hypercapnia and hypothermia
Authors: Strapazzon Giacomo , Taboni Anna , Dietrichs Erik Sveberg , Luks Andrew M. , Brugger Hermann 
Published: July 2024

AbstractFor often unclear reasons, the survival times of critically buried avalanche victims vary widely from minutes to hours. Individuals can survive and sustain organ function if they can breathe under the snow and maintain sufficient delivery of oxygen and efflux of carbon dioxide. We review the physiological responses of humans to critical avalanche burial, a model which shares similarities and differences with apnoea and accidental hypothermia. Within a few minutes of burial, an avalanche victim is exposed to hypoxaemia and hypercapnia, which have important effects on the respiratory and cardiovascular systems and pose a major threat to the central nervous system. As burial time increases, an avalanche victim also develops hypothermia. Despite progressively reduced metabolism, reduced oxygen and increased carbon dioxide tensions may exacerbate the pathophysiological consequences of hypothermia. Hypercapnia seems to be the main cause of cardiovascular instability, which, in turn, is the major reason for reduced cerebral oxygenation despite reductions in cerebral metabolic activity caused by hypothermia. ‘Triple H syndrome’ refers to the interaction of hypoxia, hypercapnia and hypothermia in a buried avalanche victim. Future studies should investigate how the respiratory gases entrapped in the porous snow structure influence the physiological responses of buried individuals and how haemoconcentration, blood viscosity and cell deformability affect blood flow and oxygen delivery. Attention should also be devoted to identifying strategies to prolong avalanche survival by either mitigating hypoxia and hypercapnia or reducing core temperature so that neuroprotection occurs before the onset of cerebral hypoxia.
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A Geometric Derivation of the Governing Equations of Motion of Nonholonomic Dynamic Systems
Authors: Liu Xiaobo 
Published: July 2024

Abstract
In this paper, we present a Riemannian geometric derivation of the governing equations of motion of nonholonomic dynamic systems. A geometric form of the work-energy principle is first derived. The geometric form can be realized in appropriate generalized quantities, and the independent equations of motion can be obtained if the subspace of generalized speeds allowable by nonholonomic constraints can be determined. We provide a geometric perspective of the governing equations of motion and demonstrate its effectiveness in studying dynamic systems subjected to nonholonomic constraints.
A customizable and defined medium supporting culturing of
Candida albicans
,
Staphylococcus aureus
, and human oral epithelial cells
Authors: Pasman Raymond , Zhang Jianbo , Zaat Sebastian A. J. , Brul Stanley , Krom Bastiaan P. 
Published: July 2024

ABSTRACT

Candida albicans,
an opportunistic oral pathogen, synergizes with
Staphylococcus aureus
, allowing bacteria to co-invade and systemically disseminate within the host. Studying human–microbe interactions creates the need for a universal culture medium that supports fungal, bacterial, and human cell culturing, while allowing sensitive analytical approaches such as OMICs and chromatography techniques. In this study, we established a fully defined, customizable adaptation of Dulbecco’s modified Eagle medium (DMEM), allowing multi-kingdom culturing of
S. aureus
,
C. albicans
, and human oral cell lines, whereas minimal version of DMEM (mDMEM) did not support growth of
S. aureus
, and neither did supplementation with dextrose, MEM non-essential amino acids, pyruvate, and Glutamax. This new medium composition, designated as “mDMEM-DMP,” promoted growth of all tested
S. aureus
strains. Addition of 25 mM 4-(2-hydroxyethyl)−1-piperazineethanesulfonic acid (HEPES) further improved growth, while higher concentrations did not improve growth any further. Higher concentrations of HEPES did result in prolonged stabilization of medium pH. mDMEM-DMP promoted (hyphal)
C. albicans
monoculturing and co-culturing on both solid and semi-solid surfaces. In contrast to
S. aureus
, addition of HEPES reduced
C. albicans
maximum culture optical density (OD). Finally, only buffered mDMEM-DMP (100 mM HEPES) was successful in maintaining the metabolic activity of human oral Ca9-22 and HO1N1 cell lines for 24 hours. Altogether, our findings show that mDMEM-DMP is a versatile and potent culture medium for both microbial and human cell culturing, providing a customizable platform to study human as well as microbial molecular physiology and putative interactions.

IMPORTANCE

Interaction between microbes and the host are in the center of interest both in disease and in health. In order to study the interactions between microbes of different kingdoms and the host, alternative media are required. Synthetic media are useful as they allow addition of specific components. In addition, well-defined media are required if high-resolution analyses such as metabolomics and proteomics are desired. We describe the development of a synthetic medium to study the interactions between
C. albicans, S. aureus,
and human oral epithelial cells. Our findings show that mDMEM-DMP is a versatile and potent culture medium for both microbial and human cell culturing, providing a customizable platform to study human as well as microbial molecular physiology and putative interactions.
A single-layer spot assay for easy, fast, and high-throughput quantitation of phages against multidrug-resistant Gram-negative pathogens
Authors: Paranos Paschalis , Pournaras Spyros , Meletiadis Joseph 
Published: July 2024

ABSTRACT

Double-layer agar (DLA) overlay plaque assay is the gold standard for phage enumeration. However, it is cumbersome and time-consuming. Given the great interest in phage therapy, we explored alternative assays for phage quantitation. A total of 16 different phages belonging to Myoviridae, Siphoviridae, and Podoviridae families were quantitated with five
K
.
pneumoniae,
eight
P
.
aeruginosa,
and three
A
.
baumannii
host isolates. Phages were quantitated with the standard DLA assay (10 mL of LB soft agar 0.7% on LB hard agar 1.5%) and the new single-layer agar (SLA) assay (10 mL of LB soft agar 0.7%) with phages spread (spread) into or spotted (spot) onto soft agar. Phage concentrations with each assay were correlated with the standard assay, and the relative and absolute differences between each assay and the standard double-layer agar spread were calculated. Phage concentrations 1 × 10
4
–8.3 x10
12
PFU/mL with the standard DLA assay were quantitated with SLA-spread, SLA-spot, and DLA-spot assays, and the median (range) relative and absolute differences were <10% and <0.98 log
10
PFU/mL, respectively, for all phage/bacterial species (ANOVA
P
= 0.1–0.43), and they were highly correlated (r > 0.77,
P
< 0.01). Moreover, plaques could be quantified at 37°C after 4-h incubation for
K. pneumoniae
phages and 6-h incubation for
P. aeruginosa
and
A. baumannii
phages, and estimated concentrations remained the same over 24 hours. Compared to DLA assay, the SLA-spot assay required less media, it was 10 times faster, and generated same-day results. The SLA-spot assay was cheaper, faster, easier to perform, and generated similar phage concentrations as the standard DLA-spread assay.
Assessing the American public’s preferences for reforms to teen driving licensure systems: a discrete choice experiment
Authors: Drabo Emmanuel Fulgence , Michael Jeffrey Paul , Ehsani Johnathon Pouya 
Published: July 2024

ObjectivesTo analyse factors influencing the American public’s preferences for changes to teenage driver licensing requirements.MethodsWe employed a discrete choice experiment (DCE) with 808 participants from National Opinion Research Center’s AmeriSpeak panel to assess preferences for two existing elements (on-road testing and intermediate licensure period) and a new feature (driver monitoring with telematics during the intermediate licensure period) of licensing system. Multinomial and mixed logit models were used to estimate preference weights, marginal rates of substitution and the relative importance of each attribute.ResultsAmong 730 respondents who completed all DCE choice tasks, we found robust support for changes to teenage driver licensing requirements, with preferences varying by individual characteristics. Respondents expressed a high baseline support for changes to teen driving licensure policies. They favoured testing, prioritising easy tests and opposed prolonged driver monitoring and extended intermediate licensure periods. Baseline preference weights exhibited substantial heterogeneity, emphasising the diversity of public preferences. The marginal rates of substitution revealed a preference for extended driver monitoring over an extended intermediate licensure period. An easy test was valued at 2.85 times more than a hard one. The most influential attributes were the length of intermediate licence period and testing requirements, with the former twice as important.ConclusionsOur study found robust support for reforms to teenage driver licensing requirements, favouring easier on-road driving tests over an extended period of intermediate licensure and driver monitoring. Public preferences for licensing systems need to be balanced with the broader policy objectives including optimising mobility and maximising safety.
A model of pentaquarks
Authors: Germani D. , Niliani F. , Polosa A. D. 
Published: July 2024

AbstractWe describe pentaquarks as ‘baryo-charmonia’ with a color octet $$c{\bar{c}}$$

c

c

¯

core bonded to a color octet three-quark system. Fermi statistics of the light quark cloud allows to describe two pentaquark triplets: a lower one, well supported by experiment, and a higher one with strangeness. For the time being, the lowest line of the strange triplet has been experimentally identified in a $$3\sigma $$

3
σ

peak. Data also suggest two different production mechanisms for pentaquarks. We show how this can be described in the proposed scheme.