bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ro60 Requires Y3 RNA for Cell Surface Exposure and Inflammation Associated with Cardiac Manifestations of Neonatal Lupus
Authors: Reed Joanne H. , Sim Soyeong , Wolin Sandra L. , Clancy Robert M. , Buyon Jill P. 
Published: May 2013

Abstract
Cardiac neonatal lupus (NL) is presumed to arise from maternal autoantibody targeting an intracellular ribonucleoprotein, Ro60, which binds noncoding Y RNA and only becomes accessible to autoantibodies during apoptosis. Despite the importance of Ro60 trafficking in the development of cardiac NL, the mechanism underlying cell surface exposure is unknown. To evaluate the influence of Y RNA on the subcellular location of Ro60 during apoptosis and activation of macrophages, stable Ro60 knockout murine fibroblasts expressing wild-type or mutated FLAG-Ro60 were assessed. FLAG3-Ro60(K170A R174A) binds Y RNA, whereas FLAG3-Ro60(H187S) does not bind Y RNA; fibroblasts expressing these constructs showed equivalent intracellular expression of Ro60. In contrast, apoptotic fibroblasts containing FLAG3-Ro60(K170A R174A) were bound by anti-Ro60, whereas FLAG3-Ro60(H187S) was not surface expressed. RNA interference of mY3 RNA in wild-type fibroblasts inhibited surface translocation of Ro60 during apoptosis, whereas depletion of mY1 RNA did not affect Ro60 exposure. Furthermore, Ro60 was not exposed following overexpression of mY1 in the mY3-depleted fibroblasts. In an in vitro model of anti-Ro60–mediated injury, Y RNA was shown to be an obligate factor for TLR-dependent activation of macrophages challenged with anti-Ro60–opsonized apoptotic fibroblasts. Murine Y3 RNA is a necessary factor to support the surface translocation of Ro60, which is pivotal to the formation of immune complexes on apoptotic cells and a TLR-dependent proinflammatory cascade. Accordingly, the Y3 RNA moiety of the Ro60 ribonucleoprotein imparts a critical role in the pathogenicity of maternal anti-Ro60 autoantibodies.
Frontal Sinus Surgery Anterior to the Ethmoid Bulla
Authors: Ji Junfeng , Zhou Mei , Li Zeqing , Wang Tianyou , Cheng You , Wang Qiuping 
Published: May 2013

Abstract
The frontal sinus surgery is difficult to perform but the ethmoid bulla is a relative, constant landmark in the middle turbinate that can improve the surgery. The purpose of this study was to evaluate the validity, security, and predominance of approaches to the frontal sinus via the route anterior to the ethmoid bulla. The data from 370 endoscopic frontal sinus surgery cases from our center were integrated and retrospectively analyzed. Three hundred twenty-nine patients underwent frontal sinus surgery via the route anterior to the ethmoid bulla. An additional 27 patients underwent frontal sinus surgery with mini-trephination, 13 patients with the Draf II procedure, and 1 patient had applied MELP (modified endoscopic Lothrop procedure). No serious complications occurred; however, there were 3 cases of eyelid ecchymosis and 1 case of anterior ethmoid artery bleeding. In all, 319 patients (86.2%) were cured, an improvement was noted in 36 of the patients (9.7%), and there was no improvement in 15 patients (4.1%). Frontal sinus surgery via the route anterior to the ethmoid bulla is valid, relatively safe, and can be applied in most cases involving frontal disease.
Influence of Glucuronidation and Reduction Modifications of Resveratrol on its Biological Activities
Authors: Lu Dong‐Liang , Ding De‐Jun , Yan Wen‐Jing , Li Ran‐Ran , Dai Fang , Wang Qi , Yu Sha‐Sha , Li Yan , Jin Xiao‐Ling , Zhou Bo 
Published: May 2013

AbstractResveratrol (3,5,4′‐trihydroxystilbene, RES), a star among dietary polyphenols, shows a wide range of biological activities, but it is rapidly and extensively metabolized into its glucuronide and sulfate conjugates as well as to the corresponding reduced products. This begs the question of whether the metabolites of RES contribute to its in vivo biological activity. To explore this possibility, we synthesized its glucuronidation (3‐GR and 4′‐GR) and reduction (DHR) metabolites, and evaluated the effect of these structure modifications on biological activities, including binding ability with human serum albumin (HSA), antioxidant activity in homogeneous solutions and heterogeneous media, anti‐inflammatory activity, and cytotoxicity against various cancer cell lines. We found that 1) 4′‐GR, DHR and RES show nearly equal binding to HSA, mainly through hydrogen bonding, whereas 3‐GR adopts a quite different orientation mode upon binding, thereby resulting in reduced ability; 2) 3‐GR shows comparable (even equal) ability to RES in FRAP‐ and AAPH‐induced DNA strand breakage assays; DHR, 3‐GR, and 4′‐GR exhibit anti‐hemolysis activity comparable to that of RES; additionally, 3‐GR and DHR retain some degree activity of the parent molecule in DPPH.‐scavenging and cupric ion‐initiated oxidation of LDL assays, respectively; 3) compared to RES, 4′‐GR displays equipotent ability in the inhibition of COX‐2, and DHR presents comparable activity in inhibiting NO production and growth of SMMC‐7721 cells. Relative to RES, its glucuronidation and reduction metabolites showed equal, comparable, or some degree of activity in the above assays, depending on the specific compound and test model, which probably supports their roles in contributing to the in vivo biological activities of the parent molecule.
Experimental evaluation of pharmacokinetic profile and biological effect of a novel paclitaxel microcrystalline balloon coating in the iliofemoral territory of swine
Authors: Buszman Piotr P. , Milewski Krzysztof , Żurakowski Aleksander , Pajak Jacek , Jelonek Michał , Gasior Paweł , Peppas Athanasios , Tellez Armando , Granada Juan F. , Buszman Paweł E. 
Published: May 2013

BackgroundNew paclitaxel coated balloons (PCB) developments have been proposed to maintain therapeutic levels of drug in the tissue while decreasing particle release. In this series of studies, we evaluated the pharmacokinetic profile and biological effects after paclitaxel delivery via novel microcrystalline PCB coating (mcPCB, Pax®, Balton) in porcine iliofemoral arteries. Methods: Ten domestic swine were enrolled yielding 24 iliofemoral segments for evaluation. In the pharmacokinetic study, nine mcPCBs were dilated for 60 sec and animals sacrificed after 1 hr, 3 and 7 days. Studied segments were harvested and tissue paclitaxel concentration was analyzed utilizing HPLC. In the biological response evaluation, self‐expandable stents were implanted followed by post dilation with either mcPCB (n = 10) or POBA (n = 5). After 28 days, angiography was performed, animals were sacrificed and stented segments harvested for histopathological evaluation. Results: The 1‐hr, 3 and 7 days vessel paclitaxel concentrations were 152.9 ± 154.5, 36.5 ± 49.5, and 0.9 ± 0.7 ng/mg respectively. In the biological response study, stents in the mcPCB group presented lower angiographic measures of neointimal hyperplasia as expressed by late loss when compared to POBA (−0.43 ± 0.9 vs. 0.23 ± 1.2; P = 0.24) at 28 days. In the histopathological evaluation, percent area of stenosis (%AS) was reduced by 42% in the mcPCB group (P < 0.05). The healing process in mcPCB group was comparable to POBA with regard to fibrin deposition (0.7 vs. 0.7; P = ns), neointimal maturity (1.97 vs. 1.93; P = ns), inflammation score (0.92 vs. 1; P = ns) and endothelialization score (1.77 vs. 1.73; P = ns). The mcPCB group did however display a greater tendency of medial cell loss and mineralization (60% vs. 0; P = 0.08). Conclusions: Delivery of paclitaxel via a novel mcPCB resulted in low long‐term tissue retention of paclitaxel. However, this technological approach displayed reduced neointimal proliferation and favorable healing profile. © 2013 Wiley Periodicals, Inc.
ChemInform Abstract: BiN3O9, a Metastable Perovskite Phase with Bi/Vacancy Ordering: Crystal Structure and Dielectric Properties.
Authors: Mumme William G. , Grey Ian E. , Edwards Bryce , Turner Christopher , Nino Juan , Vanderah Terrell A. 
Published: May 2013

AbstractThe perovskite BiNb3O9 is a metastable phase in the Bi2O3—Nb2O5 system that forms only when cooled from a liquid phase.
Comparison of Chiral Separations of Aminophosphonic Acids and Their Aminocarboxylic Acid Analogs Using a Crown Ether Column
Authors: Barnhart Wesley W. , Xia Xiaoyang , Jensen Randy , Gahm Kyung H. 
Published: May 2013

ABSTRACTCrown ethers are capable of complexing with primary amines and have been utilized in chromatography to separate amino acid racemates. This application has been extended to resolve (1‐amino‐1‐phenylmethyl)phosphonic acid and (1‐aminoethyl)phosphonic acid racemates, along with their aminocarboxylic acid analogs (2‐phenylglycine and alanine, respectively), via a ChiroSil RCA crown ether based chiral stationary phase. Effects of the organic modifier, temperature, and acid type and concentration on retention and selectivity were also investigated. Trends in retention and selectivity varied between aminophosponic acids and their aminocarboxylic analogs. Computer modeling and 1H NMR analyses were performed to potentially gain a better understanding of interactions of the aforementioned molecules with the ChiroSil RCA chiral stationary phase. Theoretical predictions of the most stable conformations for (R)‐ and (S)‐enantiomers were compared to elution order; it was found that the elution order agreed with molecular modeling such that the longest retention correlated with the predicted most stable complex between the enantiomer and crown ether. 1H NMR demonstrated interactions of aminophosphonic and aminocarboxylic racemates with (+)‐(18‐crown‐6)‐2,3,11,12‐tetracarboxylic acid in solution and was utilized to determine enantiomeric excess of (1‐amino‐1‐phenylmethyl)phosphonic acid after its enantioenrichment via crystallization through diastereomeric salt formation with the crown ether followed by filtration. Chirality 25:369–378, 2013. © 2013 Wiley Periodicals, Inc.
Pilot Tests in Organophilic Pervaporation: Membrane, Module and Simulation – An Overall Concept
Authors: Matuschewski Heike , Schiffmann Patrick , Notzke Heiko , Wolff Thorsten , Schedler Uwe , Brinkmann Torsten , Repke Jens‐Uwe 
Published: May 2013

AbstractDie kontinuierlich wachsenden Anforderungen an Produktreinheit und ansteigende Energiekosten erfordern neue, energieeffiziente technologische Ansätze in der chemischen Industrie. Speziell die selektive Abtrennung von organischen Verbindungen aus wässrigen und organischen Mischungen wird in Zukunft eine bedeutende Rolle spielen. Die organophile Pervaporation ist eine innovative Technologie für diese Trennaufgaben. Ein Gesamtkonzept für das Design eines Membranmoduls für die Pervaporation wird präsentiert. Dieses Konzept beinhaltet die Entwicklung einer hoch‐selektiven Membran und das Design eines neuen Membranmoduls. Für die strukturierte Simulation der Trennleistung der Membran und des Membranmoduls werden drei sequentielle Modelle erstellt und in einem benutzerfreundlichen Software‐Tool implementiert.
Mapping Protein Conformational Energy Landscapes Using NMR and Molecular Simulation
Authors: Guerry Paul , Mollica Luca , Blackledge Martin 
Published: May 2013

AbstractNuclear magnetic resonance (NMR) spectroscopy provides detailed understanding of the nature and extent of protein dynamics on physiologically important timescales. We present recent advances in the combination of NMR with state‐of‐the‐art molecular simulation that are providing unique new insight into the motions on timescales from nanoseconds to milliseconds. In particular, we focus on methods based on residual dipolar couplings (RDCs) that allow for detailed mapping of the protein conformational energy landscape. A novel combination of RDCs with accelerated molecular dynamics allows for the development of ensemble representations of the underlying Boltzmann ensemble.
Selection on the Drosophila seminal fluid protein Acp62F
Authors: Wong Alex , Rundle Howard 
Published: May 2013

AbstractSperm competition and sexual conflict are thought to underlie the rapid evolution of reproductive proteins in many taxa. While comparative data are generally consistent with these hypotheses, few manipulative tests have been conducted and those that have provided contradictory results in some cases. Here, we use both comparative and experimental techniques to investigate the evolution of the Drosophila melanogaster seminal fluid protein Acp62F, a protease inhibitor for which extensive functional tests have yielded ambiguous results. Using between‐species sequence comparisons, we show that Acp62F has been subject to recurrent positive selection. In addition, we experimentally evolved populations polymorphic for an Acp62F null allele over eight generations, manipulating the opportunities for natural and sexual selection. We found that the Acp62F null allele increased in frequency in the presence of natural selection, with no effect of sexual selection.
Design and Synthesis of Oligoamide‐Based Double α‐Helix Mimetics
Authors: Kulikov Oleg V. , Thompson Sam , Xu Hai , Incarvito Christopher D. , Scott Richard T. W. , Saraogi Ishu , Nevola Laura , Hamilton Andrew D. 
Published: May 2013

AbstractAn extensive series of bis‐oligobenzamides and bis‐oligopyridylamides have been efficiently prepared and studied by X‐ray analysis and computational methods. A modular synthesis led to double α‐helix mimics bearing between two and ten branched side‐chains. The inter‐helix angle and distance can be tuned by varying the length and rigidity of the spacer, thereby reproducing the recognition domains of a range of super‐secondary structures.
BODIPY Dyes Functionalized with Pendant Cyclic and Acyclic Polyamines
Authors: Volkova Yulia A. , Brizet Bertrand , Harvey Pierre D. , Averin Alexey D. , Goze Christine , Denat Franck 
Published: May 2013

AbstractThe synthesis and characterization of a series of BODIPY derivatives substituted with acyclic and cyclic polyamines, in particular, cyclen and homocyclen, are reported. The 19F NMR, UV/Vis, and fluorescence spectroscopic data of these compounds are discussed. One compound was found to be very selective for CuII ions, which makes it a very promising system for CuII detection.
Biocompatibility of functionalized designer self‐assembling nanofiber scaffolds containing FRM motif for neural stem cells
Authors: Zou Zhenwei , Liu Ting , Li JingFeng , Li Pindong , Ding Qian , Peng Gang , Zheng Qixin , Zeng Xianlin , Wu Yongchao , Guo Xiaodong 
Published: May 2013

AbstractPeptide self‐assembling scaffolds have been widely used in tissue engineering. Much work has been focused on modifying the self‐assembling scaffolds with functional motifs for desired biological activities. We report here the development of a biological material designed specifically for neural tissue engineering (NTE). Using RADA‐16 (AcN–RADARADARADARADA–CONH2) as a base scaffold, we synthesized a 31 amino acid peptide RADA‐FRM (AcN–RADARADARADARADAGGSIDRVEPYSSTAQ–CONH2) containing the neural cell adhesion molecule (NCAM)‐derived mimetic peptide FRM (SIDRVEPYSSTAQ), which could undergo self‐assembly into a nanofiber scaffold. We tested the characterization of the nanofiber scaffold using atomic force microscopy (AFM) and accessed the rheological properties of FRM‐containing nanofiber scaffold (FRM‐NS). Then we examined its biocompatibility on neural stem cells (NSCs) from neonatal rats. Regrettably, we found that FRM‐NS had no effect on differentiation of NSCs. However, we tested that FRM‐NS was noncytotoxic. Furthermore, compared to pure RADA‐16 scaffold, we found that the designer self‐assembling peptide scaffold containing FRM motif could significantly promote NSCs proliferation and stimulate NSCs migration into the three‐dimensional scaffold. Our results indicate that the novel designer peptide scaffold containing FRM had excellent biocompatibility with NSCs and may be useful for central nervous tissue repair. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1286–1293, 2014.
Rheological studies of polysaccharides for skin scaffolds
Authors: Almeida Nalinda , Mueller Anja , Hirschi Stanley , Rakesh Leela 
Published: May 2013

AbstractPolysaccharide hydrogels are good candidates for skin scaffolds because of their inherent biocompatibility and water transport properties. In the current study, hydrogels were made from a mixture of four polysaccharides: xanthan gum, konjac gum, iota‐carrageenan, and kappa‐carrageenan. Gel formation, strength, and structure of these polysaccharides were studied using rheological and thermal techniques. All gel samples studied were strong gels at all times because of the gradual water loss. However, after 12 h of storage, elastic (G') and loss (G'') moduli of hydrogel mixture containing all the ingredients is of one to two orders of magnitude greater than that of mixtures not containing either xanthan gum or iota‐carrageenan, which confirmed the varied levels of gel strength. This is mainly due to the rate of water loss in each of these mixtures, resulting in gels of varying structures and dynamic moduli over a period of time. Iota‐carrageenan and xanthan gum differ in their effect on gel strength and stability in combination with konjac gum and kappa‐carrageenan. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 1510–1517, 2014.
Kidney Stones: A Fetal Origins Hypothesis
Authors: Howles Sarah A , Edwards Mark H , Cooper Cyrus , Thakker Rajesh V 
Published: May 2013

ABSTRACT

Kidney stones are common, with a multifactorial etiology involving dietary, environmental, and genetic factors. In addition, patients with nephrolithiasis are at greater risk of hypertension, diabetes mellitus, metabolic syndrome, and osteoporosis, although the basis for this is not fully understood. All of these renal stone–associated conditions have also been linked with adverse early-life events, including low–birth weight, and it has been suggested that this developmental effect is due to excess exposure to maternal glucocorticoids in utero. This is proposed to result in long-term increased hypothalamic-pituitary-axis activation; there are mechanisms through which this effect could also promote urinary lithogenic potential. We therefore hypothesize that the association between renal stone disease and hypertension, diabetes mellitus, metabolic syndrome, and osteoporosis may be related by a common pathway of programming in early life, which, if validated, would implicate the developmental origins hypothesis in the etiology of nephrolithiasis. © 2013 American Society for Bone and Mineral Research.
Differential effect of hypoxia on etoposide‐induced DNA damage response and p53 regulation in different cell types
Authors: Sermeus Audrey , Rebucci Magali , Fransolet Maude , Flamant Lionel , Desmet Déborah , Delaive Edouard , Arnould Thierry , Michiels Carine 
Published: May 2013

AbstractAmong the main causes of cancer cell resistance to chemotherapy are p53 mutation and hypoxic tumor microenvironment. However, the effect of hypoxia can be very different from one cell type to the other. We studied the effect of hypoxia on the etoposide‐induced cell death in two cancer cell lines, HepG2 and A549 cells. Hypoxia decreased etoposide‐induced apoptosis in HepG2 cells but not in A549 cells. Here, we evidenced two pathways, known to play important roles in cancer cell resistance, that are differently affected by hypoxia in these two cell types. First, in HepG2 cells, hypoxia decreased p53 protein level and activity by acting post‐transcriptionally and independently of HIF‐1. The results suggest an effect of hypoxia on p53 translation. On the other hand, in A549 cells, no effect of hypoxia was observed on p53 level. Secondly, hypoxia decreased DNA damage response in HepG2 cells while this was not the case in A549 cells. Indeed, a decrease in the phosphorylation level of CHK2 and H2AX with a decrease in ATM activity was observed. Importantly, these results evidenced that hypoxia can prevent cancer cell apoptosis by acting at different levels in the cell and that these effects are strongly cell‐type dependent. J. Cell. Physiol. 228: 2365–2376, 2013. © 2013 Wiley Periodicals, Inc.
MicroRNAs support the monophyly of enteropneust hemichordates
Authors: Peterson Kevin J. , Su Yi‐Hsien , Arnone Maria Ina , Swalla Billie , King Benjamin L. 
Published: May 2013

ABSTRACTUnderstanding the evolutionary history of deuterostomes requires elucidating the phylogenetic interrelationships amongst the constituent taxa. Although the monophyly and interrelationships among the three principal groups—the chordates, the echinoderms, and the hemichordates—are well established, as are the internal relationships among the echinoderm and chordate taxa, the interrelationships among the principal groups of hemichordates—the harrimaniid enteropneusts, the ptychoderid enteropneusts, and the pterobranchs—remain unresolved. Depending on the study some find enteropneusts paraphyletic with pterobranchs (e.g.,Cephalodiscus) more closely related to the harrimaniid enteropneusts (e.g.,Saccoglossus) than either are to the ptychoderid enteropneusts (e.g.,Ptychodera), whereas other studies support a monophyletic Enteropneusta. To try and resolve between these two competing hypotheses, we turned to microRNAs, small ∼22 nt non‐coding RNA genes that have been shown to shed insight into particularly difficult phylogenetic questions. Using deep sequencing we characterized the small RNA repertoires of two hemichordate species,Cephalodiscus hodgsoniandPtychodera flava, and the crinoid echinodermAntedon mediterranea, and combined our results with the described complements of the hemichordateSaccoglossus kowalevskii, the sea urchinStrongylocentrotus purpuratus, and the starfishPatiria miniata. Our data unambiguously support the monophyly of Enteropneusts asS. kowalevskiishares 12 miRNA sequences withP. flavathat are not present in theC. hodgsoniorA. mediterranealibraries, and have never been reported from another metazoan taxon. Thus, these data resolve the phylogenetic position of pterobranchs, ultimately allowing for a better understanding of body plan evolution throughout the deuterostomes.J. Exp. Zool. (Mol. Dev. Evol.) 320B: 368–374, 2013. © 2013 Wiley Periodicals, Inc.
Liver iron quantification by 3 tesla MRI: Calibration on a rabbit model
Authors: Peng Peng , Huang Zhongkui , Long Liling , Zhao Fanyu , Li Chunyan , Li Wenmei , He Taigang 
Published: May 2013

PurposeTo determine the feasibility of liver iron quantification by 3 Tesla (T) MRI using a novel iron overload rabbit model.Materials and MethodsForty‐two rabbits underwent iron dextran loading from 1 to 15 weeks. MRI signal intensity ratio (SIR) was measured using a gradient‐echo sequence, and R2(1/T2) measured using an eight‐echo spin‐echo sequence at 3T. Ex vivo hepatic pathology was obtained for all rabbits studied. Postmortem assessments of liver iron concentration (LIC) were conducted in an atomic absorption spectrophotometer. MRI measures were fitted against LIC using linear regression for 30 of the iron‐loaded rabbits. The remaining 12 iron‐loaded rabbits were used to test the prediction accuracy of the derived models.ResultsLIC was linearly correlated to both liver‐to‐muscle SIR (r = −0.845) and R2 (r = 0.965) in a range achieved in this study (LIC < 10 mg/g dry tissue) at 3T. By regression, the linear equations were determined as: (1) Y1 = 10.581−5.924X1 (Y1: LIC, X1:SIR); (2) Y2 = −1.273+0.103X2 (Y2:LIC, X2:R2). In the 12 test rabbits, the predicted LICs using the derived equations agreed well with the results obtained using the spectrophotometer.ConclusionBoth SIR and R2 are highly correlated with LIC in a novel rabbit model. MRI quantification of liver iron overload is feasible at 3T. J. Magn. Reson. Imaging 2013;38:1585–1590. © 2013 Wiley Periodicals, Inc.