bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mizoribine selectively attenuates monocyte chemoattractant protein‐1 production in cultured human glomerular mesangial cell: A possible benefit of its use in the treatment of lupus nephritis
Authors: Aizawa Tomomi , Imaizumi Tadaatsu , Tsuruga Kazushi , Watanabe Shojiro , Chiba Yuki , Matsumiya Tomoh , Yoshida Hidemi , Tanaka Hiroshi 
Published: October 2013

AbstractAimMizoribine (MZR) is a selective inhibitor of the inosine monophosphate dehydrogenase – a key enzyme in the de novo pathway of guanine nucleotides – that was developed in Japan. Besides its immunosuppressive effects, MZR has recently been reported to suppress the progression of histologic chronicity via suppression of macrophage infiltration of the interstitium in selected patients with lupus nephritis.MethodsWe examine the direct effect of MZR in human mesangial cells on the expression of functional molecules including monocyte chemoattractants in cultured human mesangial cells (MCs) treated with polyinosinic‐polycytidylic acid (poly IC), a synthetic analogue of viral dsRNA, that makes ‘pseudoviral’ infection, and analyzed the expression of target molecules by reverse transcriptase‐polymerase chain reaction and Western blotting. Thereafter, the effect of MZR on the expressions was examined.ResultsPretreatment of cells with MZR partially, but significantly, attenuates the expression of monocyte chemoattractant protein (MCP)‐1 mRNA and protein, whereas the poly IC‐induced expressions for the other functional molecules, such as CCL5, fractalkine and IL‐8 were not influenced by MZR treatment. On the other hand, pretreatment of cells with tacrolimus did not suppress the expression of MCP‐1 mRNA.ConclusionMizoribine itself selectively attenuated the expression of MCP‐1 both mRNA and protein levels in MCs treated with poly IC; that is, a possible model of ‘pseudoviral’ infection, which may be involved in the pathogenesis of lupus nephritis.
Psychophysiological responses to visceral and somatic pain in functional chest pain identify clinically relevant pain clusters
Authors: Farmer A. D. , Coen S. J. , Kano M. , Naqvi H. , Paine P. A. , Scott S. M. , Furlong P. L. , Lightman S. L. , Knowles C. H. , Aziz Q. 
Published: October 2013

AbstractBackgroundDespite chronic pain being a feature of functional chest pain (FCP) its experience is variable. The factors responsible for this variability remain unresolved. We aimed to address these knowledge gaps, hypothesizing that the psychophysiological profiles of FCP patients will be distinct from healthy subjects.Methods20 Rome III defined FCP patients (nine males, mean age 38.7 years, range 28–59 years) and 20 healthy age‐, sex‐, and ethnicity‐matched controls (nine males, mean 38.2 years, range 24–49) had anxiety, depression, and personality traits measured. Subjects had sympathetic and parasympathetic nervous system parameters measured at baseline and continuously thereafter. Subjects received standardized somatic (nail bed pressure) and visceral (esophageal balloon distension) stimuli to pain tolerance. Venous blood was sampled for cortisol at baseline, post somatic pain and post visceral pain.Key ResultsPatients had higher neuroticism, state and trait anxiety, and depression scores but lower extroversion scores vs controls (all p < 0.005). Patients tolerated less somatic (p < 0.0001) and visceral stimulus (p = 0.009) and had a higher cortisol at baseline, and following pain (all p < 0.001). At baseline, patients had a higher sympathetic tone (p = 0.04), whereas in response to pain they increased their parasympathetic tone (p ≤ 0.008). The amalgamating the data, we identified two psychophysiologically distinct ‘pain clusters’. Patients were overrepresented in the cluster characterized by high neuroticism, trait anxiety, baseline cortisol, pain hypersensitivity, and parasympathetic response to pain (all p < 0.03).Conclusions & InferencesIn future, such delineations in FCP populations may facilitate individualization of treatment based on psychophysiological profiling.
Characteristics of patients complaining of halitosis and factors associated with halitosis
Authors: Lu H‐X , Tang C , Chen X , Wong MCM , Ye W 
Published: October 2013

ObjectivesTo describe the characteristics of patients who visited halitosis clinic and to investigate the factors that may associate with halitosis.Materials and MethodsNine hundred and eleven patients, who visited halitosis clinic for consultations, underwent organoleptic tests and volatile sulfur compound (VSC) measurements with a portable sulfide monitor. The oral health of patients, including dental caries, oral hygiene, and gingival inflammation status, was assessed. Questionnaires were used to obtain information on demographics, oral hygiene practices, and dietary habits.ResultsOf the 911 patients, more females than males visited the clinic. Patients’ age ranged from 18 to 82 years (mean ± s.d.: 40.8 ± 14.1). Almost 30% of patients had complained of halitosis for over 5 years before looking into treatments. Approximately half of the patients self‐perceived that they were unable to get close to others. Around 77% of patients had halitosis. Results of multiple logistic regressions for organoleptic scores andVSCvalues showed that tongue coating and tongue scraping were significantly associated with halitosis (P < 0.05).ConclusionsAmong these Chinese patients, over three‐quarters of patients had halitosis, and negative impacts on social communications were observed. Tongue coating was the most important factor responsible for halitosis.
Molecular cytogenetic characterization of two partial wheat Elytrigia elongata amphiploids resistant to powdery mildew
Authors: He Fang , Xu Jinqiu , Qi Xiaolei , Bao Yinguang , Li Xingfeng , Zhao Fengtao , Wang Honggang 
Published: October 2013

AbstractTwo new partial amphiploids SN20 and SN122, derived from crosses between Elytrigia elongata and common wheat Yannong15 and Shannongfu63, were found resistant to powdery mildew, a serious fungal wheat disease. Cytogenetic observations showed that each SN20 and SN122 plant had 56 chromosomes. The chromosomes in most pollen mother cells formed approximately 28 bivalents, thereby showing a high degree of cytogenetic stability. Genomic in situ hybridization using St‐genomic DNA from Pseudoroegneria strigosa as a probe and ABD‐genomic DNA from Chinese Spring wheat as a blocker demonstrated that the genomic formulas for the alien chromosomes in SN20 and SN122 were 2 St + 10 JS + 2 J and 2 St + 8 JS + 4 J, respectively. These wheat E. elongata partial amphiploids represent a potential novel source of resistance to powdery mildew for wheat breeding.
Selection by seed weight improves traits related to seedling establishment in Panicum coloratum L. var. makarikariense
Authors: Giordano Mabel C. , Berone Germán D. , Tomás María A. 
Published: October 2013

AbstractSelection for heavy seed was implemented to overcome establishment problems in Panicum coloratum var. makarikariense. A collection covering the genetic diversity of the species available in Argentina was used as a base population (BP), and two cycles of recurrent phenotypic selection to increase seed weight were performed. Seedlings, obtained from seeds from each cycle and the BP, were further evaluated in the greenhouse. An increase of 17% in mean seed weight was obtained after two cycles of recurrent selection. No differences in germination or emergence were detected among the selected materials and the BP. However, seedlings from selected materials were taller and showed higher leaf elongation rate, adventitious roots elongation rate and total growth rate than that from BP. This research also revealed that in P. coloratum var. makarikariense, more vigorous seedlings can be selected from genotypes that produce heavier seeds.
Identification of novelQTLfor leaf traits in soybean
Authors: Jun Tae‐Hwan , Freewalt Keith , Michel Andy P. , Mian Rouf 
Published: October 2013

AbstractSeveral leaf traits of soybean (Glycine maxL.Merr.), including leaf area (LA), leaf shape (LS) and specific leaf weight (SLW) may be related to soybean yield. The objective of this study was to identify novel quantitative trait loci (QTL) forLA,LSandSLWin a recombinant inbred line (RIL) population. The phenotype data were collected in 2011 and 2012 for 93 F7:10RILs using a randomized complete block design with 2 replicates each year. Five hundred and sixteen single‐nucleotide polymorphism (SNP) markers and the phenotype data were used to detectQTLusing single marker analysis (SMA) and composite interval mapping (CIM). Single markers analysis identified 26QTLfor the three traits, of which 17 were novel and the rests were previously reportedQTL. Most of theseQTLwere also identified byCIM. MostQTLreported in this study were in close proximity (<1 cM) of one or moreSNPmarkers. These publicly availableSNPmarkers with close linkage toLA,LSandSLWshould be useful for marker‐assisted breeding for these traits.
Early evaluation of popcorn inbred lines for phosphorus use efficiency
Authors: Mundim Gabriel B. , Viana José Marcelo S. , Maia Ciro 
Published: October 2013

AbstractPhosphorus use efficiency (PUE) evaluation based on shoot dry weight in the early stages of plant development can be a useful measure to identify genotypes that are superior in phosphorus (P) use. The objectives of this study were to (i) identify inbred lines that are efficient and inefficient in P use, (ii) assess the genetic diversity among the inbred lines based on traits related to PUE, (iii) investigate the relationship among traits related to PUE and its components and (iv) estimate the expected responses to selection for increasing PUE. Twenty‐three S6 popcorn inbred lines were evaluated under high and low P, and several traits related to PUE and its components were measured. Efficient and inefficient inbred lines under each P level were identified, and the genetic diversity assessment grouped these inbred lines in opposite clusters. Phosphorus acquisition efficiency (PAE) was the most important component of PUE in the early stages of plant development, and the direct selection for PAE seems to be the best method for increasing PUE in these stages.
Quality of Life Impairment in Children and Adolescents with Vitiligo
Authors: Silverberg Jonathan I. , Silverberg Nanette B. 
Published: October 2013

AbstractVitiligo significantly affects quality of life (QOL) in adults, but little is known about the effect on QOL of pediatric vitiligo and whether the extent, distribution, and duration of vitiligo are associated with QOL. We performed an online parental questionnaire‐based study (N = 350) regarding children ages 0 to 17 years with vitiligo, including validated questions about body surface area (BSA), distribution, and age of onset of vitiligo, associated symptoms, and QOL using the Children's Dermatology Life Quality Index (CDLQI). Vitiligo negatively affected numerous aspects of and total CDLQI score (median 3.0, interquartile range 5.0). Their vitiligo lesions did not bother only 4.1% of teenagers ages 15 to 17 years, versus 45.6% of children ages 0 to 6 years and 50.0% of those ages 7 to 14 years (p < 0.001). There was no association between the child's age and whether the child's vitiligo bothered the parents (p = 0.27). The most bothersome sites of vitiligo lesions for children and parents were the face (25.6% and 37.4%, respectively) and legs (26.2% and 26.2%, respectively). Eighty‐two patients (30.1%) reported itching and painful skin within the past week. Using multivariate ordinal logistic regression models, it was found that an affected BSA of more than 25% was associated with self‐consciousness, difficulty with friendships and schoolwork, and teasing and bullying. Lesions on the face and arms were associated with teasing and bullying. The extent of vitiligo is associated with QOL impairment in children and adolescents, especially self‐consciousness, but also bullying and teasing. Different distributions of vitiligo lesions are associated with different aspects of QOL impairment. Teenagers ages 15 to 17 years seem to experience the most self‐consciousness of all pediatric age groups.
Tinea Capitis Mimicking Dissecting Cellulitis
Authors: Torok Rachel D. , Bellet Jane S. 
Published: October 2013

AbstractTinea capitis is a common disease of childhood that typically follows one of several clinical patterns. Our patient and several previously reported cases demonstrate the existence of a dissecting cellulitis‐like presentation of tinea capitis. This variant should be recognized to prevent misdiagnosis of dissecting cellulitis and allow proper treatment to prevent scarring alopecia.
Novel CTSK mutation resulting in an entire exon 2 skipping in a Thai girl with pycnodysostosis
Authors: Utokpat Pattarapa , Panmontha Wipa , Tongkobpetch Siraprapa , Suphapeetiporn Kanya , Shotelersuk Vorasuk 
Published: October 2013

AbstractPycnodysostosis is a rare autosomal recessive skeletal dysplasia characterized by osteosclerosis, short stature, acro‐osteolysis of the distal phalanges, bone fragility and skull deformities. Mutations in the cathepsin K (CTSK) gene, which encodes a lysosomal cysteine protease highly expressed in osteoclasts, have been found to be responsible for the disease. We identified a Thai girl with pycnodysostosis. Her parents were first cousins. Polymerase chain reaction sequencing of the entire coding regions of CTSK of the proband's complementary DNA revealed that the whole exon 2 was skipped. We subsequently amplified exon 2 using genomic DNA, which showed that the patient was homozygous for a c.120G>A mutation. The mutation was located at the last nucleotide of exon 2. Its presence was confirmed by restriction enzyme analysis using BanI. The skipping of exon 2 eliminates the normal start codon. The mutation has never been previously reported, thus the current report expands the CTSK mutational spectrum.
Young athlete with sudden cardiac arrest treated with therapeutic hypothermia
Authors: Kojima Katsuaki , Sato Akihiro , Yamashita Yukio , Ishihara Jun , Aoyagi Hideshi , Okishige Kaoru 
Published: October 2013

AbstractReported herein is a coronary anomaly that occurred in a young adolescent athlete who presented with cardiopulmonary arrest. The patient was resuscitated and treated with therapeutic hypothermia. The patient had no associated neurological complications at follow up. Enhanced computed tomography of the heart indicated an anomalous left main coronary artery originating from the right coronary sinus and coursing between the aorta and the pulmonary artery. The patient underwent surgical intervention with coronary artery bypass grafting to prevent symptom recurrence.
Intussusception secondary to anaphylactic reaction to salmon roe (ikura)
Authors: Takeuchi Masato , Oda Yoichiro , Suzuki Isao 
Published: October 2013

AbstractAnaphylactic food reaction often involves gastrointestinal symptoms, such as vomiting and abdominal pain, but to date, there have been no publications documenting the association between food hypersensitivity and intussusception. Herein is reported the case of a 2‐year‐old boy with intussusception accompanied by anaphylactic food reaction. The patient without known allergies complained of severe abdominal pain following ingestion of salmon roe for the first time. Dyspnea, wheezing and generalized urticaria also developed. Subsequently, he had stools containing jelly‐like blood with mucus. At hospital arrival, physical examination identified an abdominal mass in the right lower quadrant; imaging confirmed the diagnosis of colo‐colic intussusception. This patient was successfully treated with enema and no pathological findings were identified via radiology. Laboratory results supported the presence of IgE‐mediated allergy to salmon roe in the present patient. To our knowledge, this is the first report to describe the possible association between intussusception and a hypersensitive food reaction.
Cardiovascular risk factors in children after kidney transplantation – From short‐term to long‐term follow‐up
Authors: Kaidar Maital , Berant Michael , Krauze Irit , Cleper Roxana , Mor Eitan , Bar‐Nathan Nathan , Davidovits Miriam 
Published: October 2013

AbstractCardiovascular‐related mortality is 100‐fold higher in pediatric renal transplant recipients than in the age‐matched general population. Seventy‐seven post‐renal transplant children's charts were reviewed for cardiovascular risk factors at two and six months after transplantation (short term) and at two yr after transplantation and the last follow‐up visit (mean 7.14 ± 3.5 yr) (long term). Significant reduction was seen in cardiovascular risk factors prevalence from two months after transplantation to last follow‐up respectively: Hypertension from 52.1% to 14%, hypercholesterolemia from 48.7% to 33%, hypertriglyceridemia from 50% to 12.5%, anemia from 29.6% to 18.3%, hyperparathyroidism from 32% to 18.3% and hyperglycemia from 11.7% to 10%, and left ventricular hypertrophy from 25.8% at short term to 15%. There was an increase in the prevalence of obesity from 1.5% to 3.9% and of CKD 3–5 from 4.75% to 24%. The need for antihypertensive treatment decreased from 54% to 42%, and the percentage of patients controlled by one medication rose from 26% to 34%, whereas the percentage controlled by 2, 3, and 4 medications decreased from 21.9%, 5.5%, and 1.4% to 6%, 2%, and 0. Children after renal transplantation appear to have high rates of cardiovascular risk factors, mainly on short‐term follow‐up.
Assessment of pollen reward and pollen availability in Solanum stramoniifolium and Solanum paniculatum for buzz‐pollinating carpenter bees
Authors: Burkart A. , Schlindwein C. , Lunau K. 
Published: October 2013

AbstractThe two widespread tropical Solanum species S. paniculatum and S. stramoniifolium are highly dependent on the visits of large bees that pollinate the flowers while buzzing them. Both Solanum species do not offer nectar reward; the rewarding of bees is thus solely dependent on the availability of pollen. Flower visitors are unable to visually assess the amount of pollen, because the pollen is hidden in poricidal anthers. In this study we ask whether and how the amount of pollen determines the attractiveness of flowers for bees. The number of pollen grains in anthers of S. stramoniifolium was seven times higher than in S. paniculatum. By contrast, the handling time per five flowers for carpenter bees visiting S. paniculatum was 3.5 times shorter than of those visiting S. stramoniifolium. As a result foraging carpenter bees collected a similar number of pollen grains per unit time on flowers of both species. Experimental manipulation of pollen availability by gluing the anther pores showed that the carpenter bees were unable to detect the availability of pollen by means of chemical cues before landing and without buzzing. Our study shows that the efficiency of pollen collecting on S. paniculatum is based on large inflorescences with short between‐flower search times and short handling time of individual flowers, whereas that of S. stramoniifolium relies on a large amount of pollen per flower. Interestingly, large carpenter bees are able to adjust their foraging behaviour to drastically different strategies of pollen reward in otherwise very similar plant species.
Hyperventilation and exhaustion syndrome
Authors: Ristiniemi Heli , Perski Aleksander , Lyskov Eugene , Emtner Margareta 
Published: October 2013

Chronic stress is among the most common diagnoses in Sweden, most commonly in the form of exhaustion syndrome (ICD‐10 classification – F43.8). The majority of patients with this syndrome also have disturbed breathing (hyperventilation). The aim of this study was to investigate the association between hyperventilation and exhaustion syndrome. Thirty patients with exhaustion syndrome and 14 healthy subjects were evaluated with the Nijmegen Symptom Questionnaire (NQ). The participants completed questionnaires about exhaustion, mental state, sleep disturbance, pain and quality of life. The evaluation was repeated 4 weeks later, after half of the patients and healthy subjects had engaged in a therapy method called ‘Grounding’, a physical exercise inspired by African dance. The patients reported significantly higher levels of hyperventilation as compared to the healthy subjects. All patients’ average score on NQ was 26.57 ± 10.98, while that of the healthy subjects was 15.14 ± 7.89 (t = −3.48, df = 42, p < 0.001). The NQ scores correlated strongly with two measures of exhaustion (Karolinska Exhaustion Scale KES r = 0.772, p < 0.01; Shirom Melamed Burnout Measure SMBM r = 0.565, p < 0.01), mental status [Hospital Anxiety and Depression Score (HADS) depression r = 0.414, p < 0.01; HADS anxiety r = 0.627, p < 0.01], sleep disturbances (r = −0.514, p < 0.01), pain (r = −.370, p < 0.05) and poor well‐being (Medical Outcomes Survey Short Form 36 questionnaire‐ SR Health r = −0.529, p < 0.05). In the logistic regression analysis, the variance in the scores from NQ were explained to a high degree (R2 = 0.752) by scores in KES and HADS. The brief Grounding training contributed to a near significant reduction in hyperventilation (F = 2.521, p < 0.124) and to significant reductions in exhaustion scores and scores of depression and anxiety. The conclusion is that hyperventilation is common in exhaustion syndrome patients and that it can be reduced by systematic physical therapy such as Grounding.
Bone mineral density, growth, pubertal development and other parameters in Brazilian children and young adults with sickle cell anaemia
Authors: Meeuwes M. , Souza de Carvalho T. F. , Cipolotti R. , Gurgel R. Q. , Ferrão T. O. , Peters M. , Agyemang C. 
Published: October 2013

AbstractObjectivesTo evaluate the occurrence of low bone mineral density (BMD) and its relationship with clinical and laboratorial characteristics in children and young adults with sickle cell anaemia living in Northeast‐Brazil, and to assess the role of radiography in diagnosing low BMD.MethodsBone mineral density of lumbar spine was measured by dual energy X‐ray absorptiometry (DXA) in 27 patients with Sickle cell anaemia (SCA) aged 7–28 years. Clinical history, calcium and calorie intake, laboratory measurements, anthropometrics and pubertal development were assessed, and X‐rays were obtained. Z‐scores and T‐scores for weight, height, Body Mass Index (BMI) and BMD were calculated using age and gender matched reference data.ResultsMean lumbar spine BMD Z‐scores and T‐scores were −1.81 SD in boys and −0.80 SD in girls. BMD Z‐scores were below ‐2 SD in 33.3% of girls and in 46.7% of boys. Low BMD (<−2 SD) occurred significantly more in patients with low height‐for‐age (P = 0.02), low weight‐for‐age (P = 0.001) and low BMI‐for‐age (P = 0.006). No significant relationships were found between BMD and other clinical and laboratory parameters. Radiography had a sensitivity of 75% and a specificity of 36% to detect low BMD, and was considered not useful in this context.ConclusionsPatients with low height and/or low weight‐for‐age seem to be at high risk for developing low BMD.
Babesia canis vogeli,Ehrlichia canis, and Anaplasma platys infection in a dog
Authors: Al Izzi Salah , Martin Donald S. , Chan Roxanne Y.Y. , Leutenegger Christian M. 
Published: October 2013

AbstractA 12‐month‐old male neutered mixed breed dog was presented with a history of diarrhea, lethargy, emaciation, polydypsia, and sniffling. Physical examination findings included pale mucous membranes, increased heart and respiratory rates, and normal rectal temperature (38°C). Hematologic abnormalities included anemia and thrombocytopenia. Biochemical abnormalities included hypoalbuminemia, hyperbilirubinemia, and elevated ALP and ALT activities. A SNAP 4Dx test result was positive for Ehrlichia canis. Babesia canis vogeli organisms were found in the peripheral blood films, while morulae of E canis were not seen. Real‐time polymerase chain reaction testing confirmed the presence of both B c vogeli and E canis organisms, and also was positive for Anaplasma platys infection. The dog recovered following treatment with doxycycline and imidocarb dipropionate, with normal hematology and biochemical profiles.