bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of DNA adductomics to soil bacterium Sphingobium sp. strain KK22
Authors: Kanaly Robert A. , Micheletto Ruggero , Matsuda Tomonari , Utsuno Youko , Ozeki Yasuhiro , Hamamura Natsuko 
Published: August 2015

AbstractToward the development of ecotoxicology methods to investigate microbial markers of impacts of hydrocarbon processing activities, DNA adductomic analyses were conducted on a sphingomonad soil bacterium. From growing cells that were exposed or unexposed to acrolein, a commonly used biocide in hydraulic fracturing processes, DNA was extracted, digested to 2′‐deoxynucleosides and analyzed by liquid chromatography‐positive ionization electrospray‐tandem mass spectrometry in selected reaction monitoring mode transmitting the [M + H]+ > [M + H − 116]+ transition over 100 transitions. Overall data shown as DNA adductome maps revealed numerous putative DNA adducts under both conditions with some occurring specifically for each condition. Adductomic analyses of triplicate samples indicated that elevated levels of some targeted putative adducts occurred in exposed cells. Two exposure‐specific adducts were identified in exposed cells as 3‐(2′‐deoxyribosyl)‐5,6,7,8‐tetrahydro‐6‐hydroxy‐(and 8‐hydroxy‐)pyrimido[1,2‐a]‐ purine‐(3H)‐one (6‐ and 8‐hydroxy‐PdG) following synthesis of authentic standards of these compounds and subsequent analyses. A time course experiment showed that 6‐ and 8‐hydroxy‐PdG were detected in bacterial DNA within 30 min of acrolein exposure but were not detected in unexposed cells. This work demonstrated the first application of DNA adductomics to examine DNA damage in a bacterium and sets a foundation for future work.
Flow‐through anastomosis for both the artery and vein in leg free flap transfer
Authors: Fujiki Masahide , Miyamoto Shimpei , Sakuraba Minoru 
Published: August 2015

ObjectiveFree flap transfer is an essential part of limb‐sparing surgery for leg sarcoma; however, this procedure is associated with a high failure rate. The aim of this study was to identify factors that contribute to microvascular compromise and flap failure for leg free flap transfer, while focusing on anastomotic techniques (end‐to‐end, end‐to‐side, and flow‐through anastomoses).Patients and methodsData from 56 consecutive patients who underwent leg free flap transfer after oncologic resection were retrospectively reviewed. Of these patients, flow‐through anastomosis was performed with the artery in 29 and the vein in 24, whereas conventional techniques were performed in others. The variables contributing to microvascular compromise and flap failure were statistically analyzed.ResultsVenous thrombosis (four patients, 7.1%) was more frequent than arterial thrombosis (one patient, 1.8%) and (4 patients, 7.1%) resulted in flap loss. All anastomotic failures occurred in end‐to‐end anastomoses. All flow‐through anastomoses achieved patency both for the artery and vein. No anastomotic failure or flap loss occurred in 22 flaps which were transferred with flow‐through anastomosis for both the artery and vein. Flow‐through venous anastomosis tended to have a lower rate of microvascular compromise and flap loss than conventional techniques (P = 0.13 and 0.25, respectively).ConclusionThe key to successful leg free flap transfer is to prevent venous failure; thus, flow‐through venous anastomosis may be a breakthrough solution. Preferential use of flow‐through anastomosis for both the artery and vein can provide predictable results in leg free flap transfer. © 2015 Wiley Periodicals, Inc. Microsurgery 35:536–540, 2015.
The effect of differing patterns of childhood body mass index gain on adult physiology in American Indians
Authors: Thearle Marie S. , Votruba Susanne B. , Piaggi Paolo , Muller Yunhua L. , Hanson Robert L. , Baier Leslie J , Knowler William , Krakoff Jonathan 
Published: August 2015

ObjectiveIdentifying groups of individuals with similar patterns of body mass index (BMI) change during childhood may increase understanding of the relationship between childhood BMI and adult health.MethodsDiscrete classes of BMI z‐score change were determined in 1,920 American Indian children with at least four non diabetic health examinations between the ages of 2 and 18 years using latent class trajectory analysis. In subsets of subjects, data were available for melanocortin‐4 receptor (MC4R) sequencing; in utero exposure to type 2 diabetes (T2D); or, as adults, oral glucose tolerance tests, onset of T2D, or body composition.ResultsSix separate groups were identified. Individuals with a more modern birth year, an MC4R mutation, or in utero exposure to T2D were clustered in the two groups with high increasing and chronic overweight z‐scores (P < 0.0001). The z‐score classes predicted adult percent fat (P < 0.0001, partial r2 = 0.18 adjusted for covariates). There was a greater risk for T2D, independent from adult BMI, in three classes (lean increasing to overweight, high increasing, and chronic overweight z‐scores) compared to the two leanest groups (respectively: HRR= 3.2, P = 0.01; 6.0, P = 0.0003; 11.6, P < 0.0001).ConclusionsDistinct patterns of childhood BMI z‐score change associate with adult adiposity and may impact risk of T2D.
Reliability of the Dutch translation of the Kujala Patellofemoral Score Questionnaire
Authors: Ummels P. E. J. , Lenssen A. F. , Barendrecht M. , Beurskens A. J. H. M. 
Published: August 2015

AbstractObjectiveThere are no Dutch language disease‐specific questionnaires for patients with patellofemoral pain syndrome available that could help Dutch physiotherapists to assess and monitor these symptoms and functional limitations. The aim of this study was to translate the original disease‐specific Kujala Patellofemoral Score into Dutch and evaluate its reliability.MethodsThe questionnaire was translated from English into Dutch in accordance with internationally recommended guidelines. Reliability was determined in 50 stable subjects with an interval of 1 week. The patient inclusion criteria were age between 14 and 60 years; knowledge of the Dutch language; and the presence of at least three of the following symptoms: pain while taking the stairs, pain when squatting, pain when running, pain when cycling, pain when sitting with knees flexed for a prolonged period, grinding of the patella and a positive clinical patella test. The internal consistency, test–retest reliability, measurement error and limits of agreement were calculated.ResultsInternal consistency was 0.78 for the first assessment and 0.80 for the second assessment. The intraclass correlation coefficient (ICCagreement) between the first and second assessments was 0.98. The mean difference between the first and second measurements was 0.64, and standard deviation was 5.51. The standard error measurement was 3.9, and the smallest detectable change was 11. The Bland and Altman plot shows that the limits of agreement are −10.37 and 11.65.ConclusionsThe results of the present study indicated that the test–retest reliability translated Dutch version of the Kujala Patellofemoral Score questionnaire is equivalent of the test–retest original English language version and has good internal consistency. Trial registration NTR (TC = 3258). Copyright © 2015 John Wiley & Sons, Ltd.
The Roles of Photography for Developing Literacy Across the Disciplines
Authors: Cappello Marva , Lafferty Karen E. 
Published: August 2015

AbstractTeachers can capitalize on the overwhelmingly visual nature of contemporary society for learning and teaching through integrating photography in their classroom instruction. In offering an alternative pathway for acquiring and expressing knowledge, photography has the potential to strengthen instruction across disciplines by drawing on multiple literacies. Indeed, photography can magnify and transform how students learn. This article explores the roles photography played in one fourth grade science classroom where teachers used Visual Thinking Strategies to promote close reading of visual texts and students created photo‐booklets to demonstrate disciplinary literacy in geology as well as reflect on their acquisition of content knowledge through photo‐interviews. In de‐scribing an instructional sequence of visual‐based activities and multi‐modal projects, this article illustrates photography's potential to improve disciplinary literacy teaching and learning by facilitating metacognition, academic vocabulary, and risk taking.
Prenatal ultrasound evaluation of segmental level of neurological lesion in fetuses with myelomeningocele: development of a new technique
Authors: Carreras E. , Maroto A. , Illescas T. , Meléndez M. , Arévalo S. , Peiró J. L. , García‐Fontecha C. G. , Belfort M. , Cuxart A. 
Published: August 2015

ABSTRACTObjectivesTo report our preliminary experience in the use of prenatal ultrasound examination to assess lower‐limb movements in fetuses with myelomeningocele. We aimed to determine the accuracy of this method to establish the segmental level of neurological lesion, as this is the best known predictor of the future ability to walk.MethodsThis was a preliminary, observational study including fetuses with myelomeningocele operated on prenatally. The patterns of movements present and absent in the affected fetuses' lower limbs were evaluated systematically by ultrasound examination. According to the known nerve function associated with each muscle group, the segmental level of neurological lesion was established before birth. The agreement for the segmental levels assigned, between the prenatal ultrasound technique and the classical neurological clinical examination after birth (gold standard), was tested using the weighed kappa (wκ) index.ResultsSeventy‐one fetuses with myelomeningocele were evaluated at the Hospital Universitari Vall d'Hebron. After counseling, the parents opted for prenatal surgery (26 cases), termination of pregnancy (43 cases) or postnatal repair (two cases). Five patients did not fulfil the inclusion criteria for prenatal surgery and three were excluded after birth. In the 18 fetuses that underwent surgery and were analyzed, the agreement between prenatal and postnatal segmental levels assigned was 91.7% for the right limb (wκ = 0.80) and 88.9% for the left limb (wκ = 0.73).ConclusionsThe agreement found between prenatal and postnatal assignment of level of lesion in this preliminary study suggests that neurological sonographic evaluation is feasible before birth. This may provide accurate individualized information about the motor function and future ambulation prognosis of fetuses with myelomeningocele. Copyright © 2015 ISUOG. Published by John Wiley & Sons Ltd.
Clinical Significance of Multiple Pulmonary Metastasectomy for Hepatocellular Carcinoma
Authors: Mizuguchi Shinjiro , Nishiyama Noritoshi , Izumi Nobuhiro , Tsukioka Takuma , Komatsu Hiroaki , Iwata Takashi , Tanaka Shogo , Takemura Shigekazu , Kubo Shoji 
Published: August 2015

AbstractBackground
The lung is the most common site of extrahepatic metastasis from hepatocellular carcinoma (HCC). The aim of this study was to evaluate the significance and long‐term outcomes of pulmonary metastasectomy for HCC, especially in patients with multiple nodules or repeated pulmonary recurrence.MethodsWe retrospectively analyzed 19 patients who underwent pulmonary metastasectomy for HCC at our institution from 1993 to 2013.ResultsNo in‐hospital mortality occurred. The 19 patients included 14 men. The median age was 61 (range 20–76) years. Eight patients (42 %) had single pulmonary metastatic lesions, whereas 4 (21 %) had >10 lesions. Median follow‐up after pulmonary metastasectomy was 23.1 (6.3–230) months. Twelve patients died, and the cause of death was HCC progression in nine. The 1‐, 3‐, 5‐, and 10‐year overall survival rates after pulmonary metastasectomy were 89, 48, 48, and 21 %, respectively. Seven patients developed pulmonary recurrence after initial pulmonary metastasectomy. Five of the seven underwent repeat metastasectomy, with a median survival time of 65 months, and 2‐ and 3‐year survival rates of 100 and 67 %, respectively. The 2‐ and 3‐year survival rates in the four patients with >10 pulmonary nodules were 75 and 50 %, respectively.ConclusionsSurgical resection is a safe and effective treatment in selected patients with pulmonary metastasis from HCC, even in those with multiple nodules. Repeated locoregional therapy for lung recurrence might help to improve survival in these patients.
Brokering the Research–Practice Gap: A typology
Authors: Neal Jennifer Watling , Neal Zachary P. , Kornbluh Mariah , Mills Kristen J. , Lawlor Jennifer A. 
Published: August 2015

AbstractDespite widespread recognition of a research–practice gap in multiple service sectors, less is known about how pre‐existing communication channels facilitate the flow of information between researchers and practitioners. In the current study, we applied an existing typology of brokerage developed by Gould and Fernandez (Sociol Methodol 19:89–126,1989) to examine what types of brokerage facilitate information spread between researchers and educational practitioners. Specifically, we conducted semi‐structured interviews with 19 school administrators and staff in two public school districts regarding their experiences searching for information about instructional, health, and social skills programs. Using deductive content analysis, we found evidence of all five types of brokerage identified by Gould and Fernandez (1989). However, only three types of brokerage—gatekeepers, representatives, and liaisons—were involved in the flow of information between school administrators and researchers. Moreover, information transfer often occurred in longer chains that involved multiple, distinct types of brokerage. We conclude with the broad implications of our findings for narrowing the research–practice gap by improving researchers’ dissemination efforts and practitioners’ search for information.
Puzzling role of genetic risk factors in human longevity: “risk alleles” as pro-longevity variants
Authors: Ukraintseva Svetlana , Yashin Anatoliy , Arbeev Konstantin , Kulminski Alexander , Akushevich Igor , Wu Deqing , Joshi Gaurang , Land Kenneth C. , Stallard Eric 
Published: August 2015

Abstract
Complex diseases are major contributors to human mortality in old age. Paradoxically, many genetic variants that have been associated with increased risks of such diseases are found in genomes of long-lived people, and do not seem to compromise longevity. Here we argue that trade-off-like and conditional effects of genes can play central role in this phenomenon and in determining longevity. Such effects may occur as result of: (i) antagonistic influence of gene on the development of different health disorders; (ii) change in the effect of gene on vulnerability to death with age (especially, from “bad” to “good”); (iii) gene–gene interaction; and (iv) gene–environment interaction, among other factors. A review of current knowledge provides many examples of genetic factors that may increase the risk of one disease but reduce chances of developing another serious health condition, or improve survival from it. Factors that may increase risk of a major disease but attenuate manifestation of physical senescence are also discussed. Overall, available evidence suggests that the influence of a genetic variant on longevity may be negative, neutral or positive, depending on a delicate balance of the detrimental and beneficial effects of such variant on multiple health and aging related traits. This balance may change with age, internal and external environments, and depend on genetic surrounding. We conclude that trade-off-like and conditional genetic effects are very common and may result in situations when a disease “risk allele” can also be a pro-longevity variant, depending on context. We emphasize importance of considering such effects in both aging research and disease prevention.
Mannose receptor‐mediated delivery of moss‐made α‐galactosidase A efficiently corrects enzyme deficiency in Fabry mice
Authors: Shen Jin‐Song , Busch Andreas , Day Taniqua S. , Meng Xing‐Li , Yu Chun I. , Dabrowska‐Schlepp Paulina , Fode Benjamin , Niederkrüger Holger , Forni Sabrina , Chen Shuyuan , Schiffmann Raphael , Frischmuth Thomas , Schaaf Andreas 
Published: August 2015

AbstractEnzyme replacement therapy (ERT) is an effective treatment for several lysosomal storage disorders (LSDs). Intravenously infused enzymes are taken up by tissues through either the mannose 6‐phosphate receptor (M6PR) or the mannose receptor (MR). It is generally believed that M6PR‐mediated endocytosis is a key mechanism for ERT in treating LSDs that affect the non‐macrophage cells of visceral organs. However, the therapeutic efficacy of MR‐mediated delivery of mannose‐terminated enzymes in these diseases has not been fully evaluated. We tested the effectiveness of a non‐phosphorylated α‐galactosidase A produced from moss (referred to as moss‐aGal) in vitro and in a mouse model of Fabry disease. Endocytosis of moss‐aGal was MR‐dependent. Compared to agalsidase alfa, a phosphorylated form of α‐galactosidase A, moss‐aGal was more preferentially targeted to the kidney. Cellular localization of moss‐aGal and agalsidase alfa in the heart and kidney was essentially identical. A single injection of moss‐aGal led to clearance of accumulated substrate in the heart and kidney to an extent comparable to that achieved by agalsidase alfa. This study suggested that mannose‐terminated enzymes may be sufficiently effective for some LSDs in which non‐macrophage cells are affected, and that M6P residues may not always be a prerequisite for ERT as previously considered.
Behavioural and emotional problems, intellectual impairment and health‐related quality of life in patients with organic acidurias and urea cycle disorders
Authors: Jamiolkowski Dagmar , Kölker Stefan , Glahn Esther M. , Barić Ivo , Zeman Jiri , Baumgartner Matthias R. , Mühlhausen Chris , Garcia‐Cazorla Angels , Gleich Florian , Haege Gisela , Burgard Peter ,   on behalf of the E‐IMD consortium
Published: August 2015

AbstractBackgroundOrganic acidurias (OADs) and urea cycle disorders (UCDs) are inborn metabolic disorders with a risk for acute and chronic metabolic decompensation resulting in impairments of the central nervous system and other organ systems. So far, there is no systematic study of intellectual functioning, behavioural/emotional problems and health‐related quality of life (HRQoL), and how these domains are connected.MethodsData of 152 patients with OADs (n = 100) and UCDs (n = 52) from the European Registry and Network of intoxication type Metabolic Diseases (E‐IMD) using standardized instruments were compared with normative data.ResultsBehavioural/emotional problems are increased in OADs or UCDs patients by a factor of 2.5 (3.0), in female asymptomatic carriers of X‐linked inherited UCD ornithine transcarbamylase deficiency (fasOTCD) by a factor of 1.5. All groups show similar patterns of behavioural/emotional problems, not different from epidemiological data. Mental disability (IQ ≤ 70) was found in 31 % of OAD, 43 % of UCD, but not in fasOTCD subjects. HRQoL was decreased in the physical domain, but in the normal range. Behavioural/emotional problems were significantly associated with intellectual functioning (OR = 6.24, 95 %CI: 1.39–27.99), but HRQoL was independent from both variables.ConclusionsPatients with OADs and UCDs show increased frequencies of mental disability and behavioural/emotional problems. Profiles of behavioural/emotional problems were similar to epidemiological data. Intellectual disability and behavioural/emotional problems were strongly associated. Patients’ HRQoL was in the normal range, possibly compensated by coping strategies of their families. Diagnostics and clinical care of OAD/UCD patients should be improved regarding behavioural/emotional, intellectual and quality of life aspects.
The Life Course Perspective: a Guide for Genetic Counselors
Authors: Hamilton Rebekah J. , Innella Nancy A. , Bounds Dawn T. 
Published: August 2015

AbstractThis is the first article in a two part series about utilizing the life course perspective (LCP) in genetic counseling. LCP can be a useful tool for genetic counselors when counseling people with a known genetic mutation. Previous theories such as Protection Motivation Theory (PMT) and Common Sense Model of Self‐Regulation (CSMSR) examine current reactions to a positive genetic test result. LCP extends beyond the current time to explore temporal and contextual elements of the experience. A review of research revealed, LCP has been used to study the perspective of caregivers of people with Alzheimer's disease, referral for a family history of breast cancer, Mexican American caregivers of older adult, social class and cancer incidence and cancer and the sense of mastery. Incorporating LCP into a study explaining the experiences of people living with a positive test result for a genetic mutation such as the BRCA mutation provides a comprehensive exploration of this experience.
Does Electrification Spur the Fertility Transition? Evidence From Indonesia
Authors: Grimm Michael , Sparrow Robert , Tasciotti Luca 
Published: August 2015

Abstract
We analyze various pathways through which access to electricity affects fertility in Indonesia, using a district difference-in-difference approach. The electrification rate increased by 65 % over the study period, and our results suggest that the subsequent effects on fertility account for about 18 % to 24 % of the overall decline in fertility. A key channel is increased exposure to television. Using in addition several waves of Demographic and Health Surveys, we find suggestive evidence that increased exposure to TV affects, in particular, fertility preferences and increases the effective use of contraception. Reduced child mortality seems to be another important pathway.
The Geographical Scope of the EU’s Climate Responsibilities
Authors: SCOTT Joanne 
Published: August 2015

AbstractIt is increasingly common for the EU to include extraterritorial greenhouse gas emissions within the scope of its climate change laws. These measures have proved to be fiercely controversial and on more than one occasion the EU has been forced to back down. With this in mind, this paper asks how far the EU’s climate change responsibilities ought to extend geographically. In answering this question, the paper draws a distinction between first-order and second-order climate responsibilities, acknowledges the importance of the internationally agreed ‘system boundary’ guidelines adopted by the Intergovernmental Panel on Climate Change, and seeks to learn lessons from the consequentialist approach that was favoured by the EU in giving broad geographical scope to its decision to include extraterritorial aviation emissions within the scope of its emissions trading scheme.