bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transepidermal retinoic acid delivery using ablative fractional radiofrequency associated with acoustic pressure ultrasound for stretch marks treatment
Authors: Issa Maria Cláudia Almeida , de Britto Pereira Kassuga Luiza Erthal , Chevrand Natalia Stroligo , do Nascimento Barbosa Livia , Luiz Ronir Raggio , Pantaleão Luciana , Vilar Enoi Guedes , Rochael Mayra Carrijo 
Published: January 2013

AbstractBackgroundStriae distensae (SD) treatment still remains a therapeutic challenge to dermatologists. Ablative fractional laser and radiofrequency (RF) enhance skin‐drug permeability for SD treatment.ObjectiveTo clinically evaluate the efficacy and safety as well as patient's satisfaction in relation to a method using ablative fractional RF associated with retinoic acid 0.05% cream and an acoustic pressure wave ultrasound (US) in patients with alba‐type SD on the breast.Methods and MaterialsEight patients with alba‐type SD on the breast were treated with three step procedure: (1) fractional ablative RF for skin perforation; (2) topical application of retinoic acid 0.05% on the perforated skin; and (3) US was applied to enhance the retinoic acid penetration into the skin. Other eight patients with alba‐type SD on the abdominal area were submitted to RF treatment isolated without retinoic acid or US. Three of them were submitted to skin biopsies.ResultsThree patients with SD on the breast area improved from “severe” to “moderate;” two patients improved from “severe” to “mild;” two patients from “moderate” to “mild;” one patient from “marked” to “mild.” Clinical assessment demonstrated significant improvement in the appearance of SD in all patients treated with RF associated with retinoic acid 0.05% cream and US (P = 0.008), with low incidence of side effects and high level of patient's satisfaction. Among the patients treated only with RF, two patients improved from “severe” to “marked;” one patient from “marked” to “moderate;” and one patient improved from “marked” to “mild.” Four patients did not show any sort of improvement. Clinical assessment demonstrated no significant improvement in the appearance of SD treated with RF isolated with low incidence of side effects, but low‐level of patient's satisfaction.ConclusionAblative fractional RF and acoustic pressure US associated with retinoic acid 0.05% cream is safe and effective for alba‐type SD treatment. Lasers Surg. Med. 45: 81–88, 2013. © 2012 Wiley Periodicals, Inc.
Finite element analysis of thermal and acoustic processes during laser tattoo removal
Authors: Humphries Alexander , Lister Tom S. , Wright Philip A. , Hughes Mike P. 
Published: January 2013

AbstractBackground and ObjectiveQ‐switched laser therapy is commonly used for the removal of tattoos. However, despite ever increasing demand for this intervention, a better understanding of the mechanisms that result in pigment reduction is required in order to optimise outcomes and reduce the number of treatment episodes.Study DesignA finite element analysis computer simulation was developed to model the fragmentation response of ink granules during irradiation of a professional black tattoo using a Q‐switched Nd:YAG laser. Thermal and acoustic mechanisms were considered, allowing the optimal laser settings to be predicted throughout the course of treatment. Changes in the thermal properties of the ink during heating were taken into account to improve the reliability of the results obtained.ResultsThe simulated results are in close agreement with clinical observations. Thermal fragmentation was shown to be the dominant mechanism in pigment reduction when using a 6 nanoseconds pulse at 1,064 nm. In order to provide maximum clearance whilst maintaining acceptable levels of tissue thermal damage, later treatments were shown to benefit from higher fluence levels than initial treatments. Larger spot diameters were also preferable throughout the course of treatment.ConclusionsThe results from the simulation build upon previous work carried out in the field, applying ink thermal coefficients which vary with temperature for the first time. These results compliment clinical knowledge, suggesting that a proactive increase in fluence during a course of treatments is likely to improve the response to laser therapy. Lasers Surg. Med. 45: 108–115, 2013. © 2012 Wiley Periodicals, Inc.
The effect of increased maximum power output on perioperative and early postoperative outcome in photoselective vaporization of the prostate
Authors: Rieken Malte , Bonkat Gernot , Müller Georg , Wyler Stephen , Mundorff Nicole Ebinger , Püschel Heike , Gasser Thomas , Bachmann Alexander 
Published: January 2013

AbstractBackground and ObjectivePreclinical studies suggest an increased vaporization rate and speed of the 532 nm 180‐W XPS GreenLight laser (180‐W) compared with the 120‐W HPS GreenLight laser (120‐W) and the 80‐W PV GreenLight laser (80‐W). To test the clinical relevance of this observation we analyzed intraoperative data and early postoperative results after photoselective vaporization of the prostate (PVP) with the 180‐W, 120‐W, and 80‐W laser.Study Design/Materials and MethodsA retrospective pair‐to‐pair comparison was performed including 80 consecutive patients who underwent PVP for the treatment of benign prostate enlargement with the 180‐W, 120‐W, and 80‐W laser. The groups matched concerning age, prostate volume, PSA‐value, and preoperative catheterization. Primary study outcome measurement was PSA‐value reduction at 3 months; intraoperative data, perioperative complications, and early postoperative functional course were secondary study outcome measurements.ResultsEnergy application per case (kJ), preoperative prostate volume (kJ/ml) operating time (kJ/minute), and lasing time (kJ/minute) was significantly higher with the 180‐W laser. Prevalence of impaired visibility due to bleeding was comparable between the 180‐W and the 120‐W laser but significantly lower with 80‐W. Duration of hospitalization was shorter with the 180‐W laser compared to the former laser systems. During the postoperative course of 3 months voiding parameters and micturition symptoms significantly improved in all groups, the incidence of postoperative dysuria was comparable. Postoperative PSA‐value reduction was significantly higher after treatment with the 180‐W laser.ConclusionsWith the 180‐W laser, higher energy application and higher speed of tissue vaporization leads to increased tissue vaporization compared to the former 120‐W and 80‐W laser systems. Clinical efficacy and perioperative safety are maintained with the higher powered laser. Lasers Surg. Med. 45: 28–33, 2013. © 2012 Wiley Periodicals, Inc.
MYCN is recruited to the RASSF1A promoter but is not critical for DNA hypermethylation in neuroblastoma
Authors: Charlet Jessica , Szemes Marianna , Malik Karim T.A. , Brown Keith W. 
Published: January 2013

AbstractTumor suppressor genes such as RASSF1A are often epigenetically repressed by DNA hypermethylation in neuroblastoma, where the MYCN proto‐oncogene is frequently amplified. MYC has been shown to associate with DNA methyltransferases, thereby inducing transcriptional repression of target genes, which suggested that MYCN might play a similar mechanistic role in the hypermethylation of tumor suppressor genes in neuroblastoma. This study tested that hypothesis by using co‐immunoprecipitation and ChIP to investigate MYCN–DNA methyltransferase interactions, together with MYCN knock‐down and over‐expression systems to examine the effect of MYCN expression changes on gene methylation, employing both candidate gene and genome‐wide assays. We show that MYCN interacts with DNA methyltransferases and is recruited to the promoter region of RASSF1A. However, using four model systems, we showed that long‐term silencing of MYCN induces only a small loss of DNA methylation at the RASSF1A promoter in MYCN amplified neuroblastoma cell lines and over‐expression of MYCN does not induce any DNA methylation, suggesting that MYCN is not critical for DNA hypermethylation in neuroblastoma. © 2012 Wiley Periodicals, Inc.
A galenical produced from Ba‐Wei‐Die‐Huang‐Wan (THC‐002) provides resistance to the cold stress‐induced detrusor overactivity in conscious rats
Authors: Imamura Tetsuya , Ishizuka Osamu , Sudha Gautam Silwal , Lei Zhang , Hosoda Tomoka , Noguchi Wataru , Yamagishi Takahiro , Yokoyama Hitoshi , Kurizaki Yoshiki , Nishizawa Osamu 
Published: January 2013

AbstractAimsWe determined if THC‐002, a galenical produced from Ba‐Wei‐Die‐Huang‐Wan, could increase skin temperature and inhibit detrusor overactivity induced by sudden whole body cooling. Further, we determined if THC‐002 could decrease expression of transient receptor potential melastatin 8 (TRPM8) channels associated with the cold responses.MethodsHind leg skin temperature of female 10‐week‐old Sprague–Dawley rats was measured by thermal imaging. Experimental rats (n = 12) were given oral 100 mg/kg THC‐002 daily for one week, and controls (n = 12) were similarly treated with THC‐002‐free solution. Afterwards, thermal imaging and cystometric investigations of the freely moving conscious rats were performed at room temperature (RT, 27 ± 2°C) for 20 min. The rats were then transferred to a low temperature (LT, 4 ± 2°C) environment during which thermal imaging and cystometric measurements were taken at 5, 10, 20, 30, and 40 min. Afterward, the skin tissues were harvested to estimate expression levels of TRPM8 channels by immunohistochemistry and real‐time reverse‐transcription polymerase chain reaction.ResultsThe RT skin temperature of THC‐002‐treated rats was significantly higher than controls. During the first 20 min under LT, the control rats exhibited cold stress‐induced detrusor overactivity such as decreased voiding interval and bladder capacity. THC‐002 partially inhibited the detrusor overactivity patterns. During the second 20 min, skin temperature was relatively stable, and the detrusor overactivity of both groups slowly disappeared. THC‐002 significantly reduced expression of TRPM8 channel protein and mRNA.ConclusionsTHC‐002 inhibited cold stress‐induced detrusor overactivity resulting from decreasing skin temperature. Therefore, THC‐002 might provide resistance to cold stress‐exacerbated lower urinary tract symptoms. Neurourol. Urodynam. 32: 486–492, 2013. © 2012 Wiley Periodicals, Inc.
Pelvic floor muscle injuries 6 weeks post partum—an intra‐ and inter‐rater study
Authors: Stær‐Jensen Jette , Siafarikas Franziska , Hilde Gunvor , Brækken Ingeborg H. , Bø Kari , Engh Marie Ellström 
Published: January 2013

AbstractAimTo evaluate intra‐ and inter‐rater reliability when diagnosing major defects, and inter‐rater reliability of diagnosing minor defects and muscle thickness of the pubovisceral muscle in primiparous women 6 weeks after vaginal delivery, using 3D/4D transperineal ultrasound.MethodsForty primiparous women were assessed using 3D/4D transperineal ultrasound. Volumes were acquired at maximal pelvic floor muscle (PFM) contraction, and diagnosis of muscle defects were done using tomographic ultrasound imaging (TUI) of the axial plane. Thickness was measured in three central levels of TUI. The stored volumes were analyzed offline by two investigators blinded to each others' results and the women's clinical data. Cohen's kappa (κ) and percentual agreement were calculated for defects, intraclass correlations coefficient (ICC) with 95% confidence intervals were calculated for thickness.ResultsExcellent intra‐rater values were found for all major defects. Inter‐rater values for bilateral and right‐sided defects were excellent, and good for left‐sided. Agreement for minor defects was poor. Measuring thickness ICC of 0.72 was found for the left side and 0.48 for the right side, although up to half of the cases had to be excluded owing to poor demarcation of the muscle.ConclusionTomographic ultrasound imaging of the axial plane using three central slices seems to be a reliable tool for detecting major pubovisceral muscle defects shortly after childbirth. Minor defects showed low reliability. Muscle thickness measurements showed moderate reliability, but too many cases had to be excluded for this to be a useful method for determining muscle thinning 6 weeks after delivery. Neurourol. Urodynam. 32: 993–997, 2013. © 2012 Wiley Periodicals, Inc.
Subject‐controlled stimulation of dorsal genital nerve to treat neurogenic detrusor overactivity at home
Authors: Opisso Eloy , Borau Albert , Rijkhoff Nico J.M. 
Published: January 2013

AbstractAimsTo investigate the effects of subject controlled dorsal genital nerve (DGN) electrical stimulation on neurogenic detrusor overactivity (NDO) in subjects at home.MethodsSubjects underwent a 5‐day study at home with DGN stimulation. Stimulation was provided with surface electrodes placed either on the dorsal penile shaft in males and on or close to the clitoris in females. The days 1 and 5 were with no stimulation whereas days 2–4 were with stimulation. Two urodynamic studies were performed at the beginning and at the end of the study. A bladder diary was obtained.ResultsEleven subjects with NDO and with urge incontinence were included. One subject stopped the protocol before the end of the 5‐day trial and two did not undergo the second urodynamic study. The subjects showed a statistically significant increase in bladder capacities compared to baseline (P = 0.047). Mean volume per day voided significantly increased over the study within the subjects. Differences between day 1 and day 5 were statistically significant (P = 0.028).ConclusionsThe feasibility and the globally positive outcomes of the study indicate that the stimulation of the dorsal genital nerve can be an option for the treatment of the NDO. Neurourol. Urodynam. 32: 1004–1009, 2013. © 2012 Wiley Periodicals, Inc.
Involvement of stretch‐induced Rho‐kinase activation in the generation of bladder tone
Authors: Shiomi Homare , Takahashi Norio , Kawashima Yohei , Ogawa Soichiro , Haga Nobuhiro , Kushida Nobuhiro , Nomiya Masanori , Yanagida Tomohiko , Ishibashi Kei , Aikawa Ken , Yamaguchi Osamu 
Published: January 2013

AbstractAimsThe present study investigated the role of the Rho‐kinase (ROK) pathway in the maintenance of bladder tone during the storage phase, and its effects on bladder compliance.MethodsMuscle strips from isolated rat bladder (detrusor strips) were used to evaluate the effects of the ROK inhibitors Y‐27632 and HA‐1077 on resting tension, which is independent of G‐protein coupled pathways. Stretch‐induced ROK activation was assessed by measuring phosphorylation of MYPT1 (myosin phosphatase targeting subunit) using Western blotting. The effect of ROK inhibitors on bladder compliance during bladder filling was assessed in an in vitro whole bladder model.ResultsY‐27632 and HA‐1077 caused concentration‐dependent relaxation of detrusor strips. Stretch increased MYPT1‐p[Thr853] levels by approximately 1.5‐fold in normal Krebs buffer. The ROK inhibitor Y‐27632 abolished the stretch‐induced increase and reduced the level of MYPT1‐p[Thr853] to <50% of the basal values in normal Krebs buffer. Stretch‐induced phosphorylation of MYPT1 was independent of Ca2+ originating from either extracellular or intracellular stores. When tested in the isolated whole rat bladder model, HA‐1077 significantly increased bladder compliance at both 3 and 10 µM.ConclusionsThis study demonstrates that the ROK pathway is constitutively active and stretch further activates the ROK pathway, which contributes to the generation of bladder tone, thereby affecting bladder compliance. Neurourol. Urodynam. 32: 1019–1025, 2013. © 2012 Wiley Periodicals, Inc.
Validation of use of the International Consultation on Incontinence Questionnaire‐Urinary Incontinence‐Short Form (ICIQ‐UI‐SF) for impairment rating: A transversal retrospective study of 120 patients
Authors: Timmermans Luc , Falez Freddy , Mélot Christian , Wespes Eric 
Published: January 2013

AbstractAimsA urinary incontinence impairment rating must be a highly accurate, non‐invasive exploration of the condition using International Classification of Functioning (ICF)‐based assessment tools. The objective of this study was to identify the best evaluation test and to determine an impairment rating model of urinary incontinence.MethodsIn performing a cross‐sectional study comparing successive urodynamic tests using both the International Consultation on Incontinence Questionnaire‐Urinary Incontinence‐Short Form (ICIQ‐UI‐SF) and the 1‐hr pad‐weighing test in 120 patients, we performed statistical likelihood ratio analysis and used logistic regression to calculate the probability of urodynamic incontinence using the most significant independent predictors. Subsequently, we created a template that was based on the significant predictors and the probability of urodynamic incontinence.ResultsThe mean ICIQ‐UI‐SF score was 13.5 ± 4.6, and the median pad test value was 8 g. The discrimination statistic (receiver operating characteristic) described how well the urodynamic observations matched the ICIQ‐UI‐SF scores (under curve area (UDA):0.689) and the pad test data (UDA: 0.693). Using logistic regression analysis, we demonstrated that the best independent predictors of urodynamic incontinence were the patient's age and the ICIQ‐UI‐SF score. The logistic regression model permitted us to construct an equation to determine the probability of urodynamic incontinence. Using these tools, we created a template to generate a probability index of urodynamic urinary incontinence.ConclusionsUsing this probability index, relative to the patient and to the maximum impairment of the whole person (MIWP) relative to urinary incontinence, we were able to calculate a patient's permanent impairment. Neurourol. Urodynam. 32: 974–979, 2013. © 2012 Wiley Periodicals, Inc.
Coping with the urge to smoke: A real‐time analysis
Authors: Merchant Gina , Pulvers Kim , Brooks Richard D. , Edwards Jessica 
Published: January 2013

AbstractSuccessfully coping with the urge to smoke is important to achieve smoking cessation. Nicotine‐dependent smokers (N = 123) were placed in a tempting setting in a laboratory, and the effectiveness of various coping strategies for resisting the urge to smoke were evaluated in real time. Latency (time between exposure to lit cigarettes and report of need to smoke) was the primary‐dependent variable, and coping strategies listed by participants after the smoking encounter served as predictors. There was a small positive relationship between cognitive‐specific strategies, such as using positive self‐talk, and latency (r = .19, p < .05), whereas there was a small negative relationship between behavioral‐general strategies, such as looking out the window, and latency (r = −.23, p < .01). Counseling approaches that include teaching cognitive‐specific strategies may help individuals cope with the urge to smoke. © 2012 Wiley Periodicals, Inc. Res Nurs Health 36:3–15, 2013
Malnutrition in pediatric patients with cancer at diagnosis and throughout therapy: A multicenter cohort study
Authors: Zimmermann Karin , Ammann Roland A. , Kuehni Claudia E. , De Geest Sabina , Cignacco Eva 
Published: January 2013

AbstractBackgroundMalnutrition is a common problem in pediatric patients with cancer. Reported prevalence varies widely and has often been assessed only in a subset of childhood types of cancer. This study aimed to describe the prevalence of malnutrition among pediatric patients newly diagnosed with cancer, to describe the occurrence and course of malnutrition during therapy and to identify factors associated with malnutrition during therapy.ProcedureIn a retrospective cohort study of 327 patients diagnosed from 2003 to 2006 in three Swiss tertiary care hospitals, weight and height measures together with patient‐, disease‐, and treatment‐related characteristics were assessed. Malnutrition was defined as body mass index (BMI) below −2 standard deviation scores (SDS) or a weight loss >10% from diagnosis. Malnutrition was assessed at diagnosis and continuously during anticancer therapy.ResultsAt diagnosis, 5.8% of the patients (19) were malnourished based on BMI. During anticancer therapy, the cumulative incidence of malnutrition rose to 22% (70 patients) after 30 days, to 36% (116 patients) after 60 days, and finally to 47% (155 patients). In these 155 patients, the median duration of malnutrition was 60 days (interquartile range, 21–122). Age above 10 years at diagnosis, BMI ≤ −1.0 SDS at diagnosis, and a diagnosis of medulloblastoma were positively associated with a higher proportion of malnutrition time during therapy.ConclusionsThe rapid increase of malnutrition after the start of treatment underlines the need to develop evidence‐based and efficient methods to provide nutritional support for children with cancer. Pediatr Blood Cancer 2013; 60: 642–649. © 2012 Wiley Periodicals, Inc.
Potential role for statins in sickle cell disease
Authors: Adam Soheir S. , Hoppe Carolyn 
Published: January 2013

AbstractThe complex pathophysiology of sickle cell disease (SCD) is remarkably similar to that observed in other chronic vascular diseases and involves multiple biologic pathways triggered by ischemia reperfusion injury, coagulation activation, and inflammation. Statins are potent lipid‐lowering agents commonly used to reduce the risk of cardiovascular disease. Independent of their lipid lowering effect, statins have been shown to down‐regulate inflammatory mediators and endothelial adhesion molecules, reduce tissue factor expression and restore nitric oxide bioavailability. The pleiotropic effects of statins make these agents attractive therapeutic candidates for SCD. This article reviews available evidence for the potential role of statins in SCD. Pediatr Blood Cancer 2013; 60: 550–557. © 2012 Wiley Periodicals, Inc.
Massive splenic infarction in an adolescent with hemoglobin S‐HPFH
Authors: Whyte Donna , Forget Bernard , Chui David H.K. , Luo Hong‐yuan , Pashankar Farzana 
Published: January 2013

AbstractHemoglobin sickle‐hereditary persistence of fetal hemoglobin (S‐HPFH) is a condition in which there is compound heterozygosity for the Hb S mutation and the HPFH deletion. These patients have no anemia, little evidence of hemolysis and generally have a benign clinical course compared to other types of sickle cell anemia. We describe a 19‐year‐old male with HbS‐HPFH who had no history of anemia or vaso‐occlusive crisis, who presented with a massive splenic infarct. We conclude that patients with HbS‐HPFH can occasionally present with severe complications and require a high level of clinical suspicion for complications when presenting to the hospital. Pediatr Blood Cancer 2013; 60: E49–E51. © 2012 Wiley Periodicals, Inc.
A worldwide collaboration to harmonize guidelines for the long‐term follow‐up of childhood and young adult cancer survivors: A report from the international late effects of Childhood Cancer Guideline Harmonization Group
Authors: Kremer Leontien C.M. , Mulder Renée L. , Oeffinger Kevin C. , Bhatia Smita , Landier Wendy , Levitt Gill , Constine Louis S. , Wallace W. Hamish , Caron Huib N. , Armenian Saro H. , Skinner Roderick , Hudson Melissa M. 
Published: January 2013

AbstractChildhood and young adult cancer survivors should receive optimum care to reduce the consequences of late effects and improve quality of life. We can facilitate achieving this goal by international collaboration in guideline development. In 2010, the International Late Effects of Childhood Cancer Guideline Harmonization Group was initiated. The aim of the harmonization endeavor is to establish a common vision and integrated strategy for the surveillance of late effects in childhood and young adult cancer survivors. With the implementation of our evidence‐based methods, we provide a framework for the harmonization of guidelines for the long‐term follow‐up of childhood and young adult cancer survivors. Pediatr Blood Cancer 2013; 60: 543–549. © 2012 Wiley Periodicals, Inc.