bluuebunny/crossref_metadata_2025_split huggingface dataset:
Quality by Design Approach for the Development and Validation of a Robust RP-HPLC Method for the Estimation of Aloe-emodin
Authors: Husain Muzammil , Agrawal Yogeeta O. 
Published: August 2024

Introduction/Objective:
The current study examines the methodical Quality by Design
(QbD) that facilitated the creation of an easy-to-use, quick, affordable, and stability-indicating reversed-
phase high-performance liquid chromatography (RP-HPLC) technique for the efficient analysis
of aloe-emodin.

Methods:
The chromatographic conditions were optimized with the Design Expert software 11.0
version, i.e., flow rate, buffer concentration, and column temperature.

Results:
The results of the linearity graph show R2 = 0.9988. The LOQ was 0.07949 μg/mL and
the LOD was 0.02623 μg/mL. According to ICH rules, the technique validation parameters were
within the allowed range. Utilizing the Design Expert 11.0 version, the Box–Behnken design experimental
design explains the relationships between flow rate, buffer concentration, and column
temperature at three distinct levels. The responses were monitored: the retention time (Rt), tailing
factor (Tf), and number of theoretical plates (NTPs).

Conclusion:
The suggested approach was appropriate for quantitative determination and may be
used in clinical pharmacokinetic investigations, biopharmaceutics, accredited testing laboratories,
and quality control departments in enterprises.
Understanding Interactions between a Potential Antimalarial ‘MAL2-11B’ and its Targets using In Silico Methods
Authors: Sandhu Komalpreet Kaur , Kaur Satinder , Hora Rachna , Mishra Prakash Chandra 
Published: August 2024

Introduction:
The 70 kDa heat shock proteins (Hsp70) are ubiquitous molecules that
play central roles in protein homeostasis. Their nucleotide-binding domains (NBD) are associated
with the J domains of 40 kDa co-chaperone ‘HSP40’ in performing their functions. Interruption
of this interaction significantly impacts the critical ATPase activity of Hsp70s, making them
dysfunctional.

Methods:
MAL2-11B is a dihydropyrimidine derivative that blocks Hsp70-Hsp40 interaction
and hence holds the potential to be used as a drug. This Hsp70 inhibitor is a structural analogue
of MAL3-101 that has proven anti-cancer and antiparasitic activity. MAL2-11B is predicted to
have better drug-likeness, solubility, and absorption properties than MAL3-101. In the present
study, we have therefore explored the potential of MAL2-11B as an antimalarial by using in silico
tools.

Results:
Molecular docking of MAL2-11B with all Plasmodium falciparum Hsp70 (PfHsp70)
proteins revealed its preferential affinity for two out of four homologs at the nucleotide-binding
site. Detailed analysis of the docked complexes helped us to predict the kind of protein-inhibitor
interactions and specific amino acid residues involved in binding.

Conclusion:
After in vitro validation, these data may be used as the groundwork for the design
and development of new inhibitors and drugs against malaria.
Exploring the Psychological Effects and Physical Exertion of Using Different Movement Interactions in Casual Exergames That Promote Active Microbreaks: Quasi-Experimental Study
Authors: Berglund Aseel , Orädd Helena 
Published: August 2024

BackgroundProlonged sedentary behavior, such as sitting or reclining, has consistently been identified as a stand-alone risk factor for heightened cardiometabolic risk and overall mortality. Conversely, interrupting sedentary periods by incorporating short, active microbreaks has been shown to mitigate the negative effects of sedentary behavior. Casual exergames, which mix elements of casual gaming with physical activity, are one prospective intervention to reduce sedentary behavior because they require physical exertion. Casual exergames have shown promise in fostering emotional and physical advantages when played in specific circumstances. However, little research exists on how different types of movement interactions impact the psychological effects as well as the physical exertion of playing casual exergames.ObjectiveThe primary aim of this work was to explore the psychological effects and physical exertion of playing casual exergames lasting 2 minutes. More precisely, the investigation focused on comparing upper body and full body movement interactions. In addition, the work examined variations in body positions, considering both standing and seated positions during upper body movement interactions.MethodsTwo casual exergames were developed and investigated through 2 quasi-experimental studies. In study 1, we investigated how players’ perceptions of control, exertion, and immersion were affected by using upper body as opposed to full body exergame controllers when playing casual exergames. In study 2, we investigated differences in positive affect, performance, enjoyment, and exertion when playing casual exergames with upper body movement interactions in seated and standing positions.ResultsStudy 1 showed that perceived control was significantly higher for upper body movement interactions than for full body movement interactions (P=.04), but there were no significant differences regarding perceived exertion (P=.15) or immersion (P=.66). Study 2 showed that positive affect increased significantly for both standing (P=.003) and seated (P=.001) gameplay. The participants in the standing gameplay group showed slightly higher actual exertion; however, there were no differences between the groups in terms of positive affect, perceived exertion, enjoyment, or performance.ConclusionsCasual exergames controlled by upper body movement interactions in seated gameplay can produce similar psychological effects and physical exertion as upper body movement interactions in standing gameplay and full body movement interactions. Therefore, upper body and seated casual exergames should not be overlooked as a suitable microbreak activity.
Elements Influencing User Engagement in Social Media Posts on Lifestyle Risk Factors: Systematic Review
Authors: Yip Yan Yee , Makmor-Bakry Mohd , Chong Wei Wen 
Published: August 2024

Background
The high prevalence of noncommunicable diseases and the growing importance of social media have prompted health care professionals (HCPs) to use social media to deliver health information aimed at reducing lifestyle risk factors. Previous studies have acknowledged that the identification of elements that influence user engagement metrics could help HCPs in creating engaging posts toward effective health promotion on social media. Nevertheless, few studies have attempted to comprehensively identify a list of elements in social media posts that could influence user engagement metrics.

Objective
This systematic review aimed to identify elements influencing user engagement metrics in social media posts by HCPs aimed to reduce lifestyle risk factors.

Methods
Relevant studies in English, published between January 2006 and June 2023 were identified from MEDLINE or OVID, Scopus, Web of Science, and CINAHL databases. Included studies were those that examined social media posts by HCPs aimed at reducing the 4 key lifestyle risk factors. Additionally, the studies also outlined elements in social media posts that influenced user engagement metrics. The titles, abstracts, and full papers were screened and reviewed for eligibility. Following data extraction, narrative synthesis was performed. All investigated elements in the included studies were categorized. The elements in social media posts that influenced user engagement metrics were identified.

Results
A total of 19 studies were included in this review. Investigated elements were grouped into 9 categories, with 35 elements found to influence user engagement. The 3 predominant categories of elements influencing user engagement were communication using supportive or emotive elements, communication aimed toward behavioral changes, and the appearance of posts. In contrast, the source of post content, social media platform, and timing of post had less than 3 studies with elements influencing user engagement.

Conclusions
Findings demonstrated that supportive or emotive communication toward behavioral changes and post appearance could increase postlevel interactions, indicating a favorable response from the users toward posts made by HCPs. As social media continues to evolve, these elements should be constantly evaluated through further research.
The Effect of Bilateral Orchiectomy on Total Prostate Specific Antigen (TPSA) Levels in Prostate Adenocarcinoma Patients
Authors: Sp.U WIKAN KURNIAWAN 
Published: August 2024

AbstractBackground: Hormonal therapy is one treatment option for prostate cancer. There are two main categories of hormone therapy: surgical therapy and non-surgical therapy. Orchiectomy is the surgical method of hormone therapy; medication supplementation is the non-surgical method. Many kinds of indicators are used to track the advancement of patients with prostate cancer receiving hormone therapy. tPSA levels are one of the markers that are regularly evaluated.Methods: In this retrospective study, PSA levels in patients with prostate adenocarcinoma cancer were evaluated before and after bilateral orchiectomy. Use the search terms "prostate cancer" and "orchiectomy" to find research subject data in the UGM Academic Hospital's medical records installation between 2020 and 2023. The study's findings will be shown in a graphic and statistical analysis while protecting the subjects' anonymity.Results: Most of the participants (n=18, 60.0%) have pre-orchiectomy tPSA level of more than 30 ng/mL, while only almost a quarter (n=7, 23.3%) of participants have post-orchiectomy tPSA level of more than 30 ng/mL. The two research groups (p<0.0001) before and after the orchiectomy procedure, significantly differ between the two research groups based on Wilcoxon paired sample test analysis.Conclusion: TPSA levels are considerably decreased by bilateral orchiectomy.
Educação do Campo e o (im) Pacto Nacional da Alfabetização na Idade Certa (PNAIC)
Authors: Ribeiro Simone , Albuquerque Andrea Serpa 
Published: August 2024

Este texto tem como objetivo refletir sobre Política Pública de Educação, e nosso foco são os encontros e desencontros entre o PNAIC e a Educação do Campo. O que nos move nesta escrita são as conversas que temos travado tanto nos encontros de formação do próprio programa quanto aquelas levantadas em nossos grupos de pesquisa: Historicamente como tem se dado a formulação de políticas de educação implementadas no campo brasileiro e, em que sentido, o PNAIC rompe ou reafirma esta história? No campo brasileiro cujas condições de letramento e até mesmo de acesso à escola são muito diversas, como se estabelece este pacto? O pacto é pela alfabetização ou pela alfabetização na idade certa? Quais as perspectivas para a formação de professores através do PNAIC? Por ser um tema ainda recente no cenário da política pública de educação, nossa contribuição objetiva manter vivo o diálogo, talvez suscitando ainda mais questões. Mas acreditamos que um dos caminhos para efetivar a construção de políticas públicas com a participa- ção dos sujeitos aos quais elas se destinam seja este. Como diria Riobaldo, personagem criado por Guimarães Rosa, ainda nos admiramos e nos animamos com a boniteza do inacabamento humano: “O senhor... Mire veja: o mais importante e bonito, do mundo, é isto: que as pessoas não estão sempre iguais, ainda não foram terminadas – mas que elas vão sempre mudando”.
Jogos no ensino-aprendizagem da geometria no Pacto Nacional pela Alfabetização na Idade Certa
Authors: Carneiro Reginaldo Fernando 
Published: August 2024

A utilização de jogos é uma possibilidade para retomar-se o ensino-aprendizagem da Geometria na escola básica, que até algum tempo atrás foi deixado em segundo plano. Assim, temos como objetivo, neste artigo, discutir algumas possibilidades e limites do jogo no ensino-aprendizagem de Geometria a partir do material de matemática do Pacto Nacional de Alfabetização na Idade Certa – PNAIC. Para tanto, apresentamos algumas reflexões sobre o caderno Jogos na alfabetização matemática que traz cinco jogos para o ensino de Geometria: Na direção certa, Trilha dos sabores, Jogo das figuras, Dominó geométrico e Equilíbrio geométrico. Esses jogos permitem trabalhar diversos direitos de aprendizagem e abordam os conteúdos de localização, deslocamento, lateralidade, conhecimento do espaço, figuras geométricas, sólidos geométricos, suas características, etc. Além disso, na proposta de utilização dos jogos, observamos algumas atividades, como os constantes questionamentos em todos os momentos do jogo, a necessidade de avaliação e a escrita de relatórios que podem levar a perda da ludicidade, da motivação e do interesse da criança pelo jogo.
Saberes Matemáticos Ancestrales: una mirada Etnomatemática al tejido en lana de ovejo
Authors: MORALES PEÑA JHOM JAIRO 
Published: August 2024

Este trabajo nació como respuesta a la desarticulación entre los conocimientos matemáticos escolares y las prácticas culturales desarrolladas en la comunidad indígena Yanacona de Caquiona, municipio de Almaguer, Cauca, Colombia. Ante esta problemática, se buscó identificar Saberes Matemáticos Ancestrales (SMA) en el tejer lana de ovejo, desde una perspectiva sociocultural enmarcada en la Etnomatemática, con enfoque cualitativo y tejido metodológico etnográfico. La identificación de SMA implicó la participación del investigador en el ambiente natural de las tejedoras, a través de la ejecución de actividades como la negociación, acceso y trabajo de campo; utilizando la observación participante y la entrevista semiestructurada como técnicas para la obtención de la información. En ese sentido, entre los hallazgos se encontró que en la práctica de tejer están presentes SMA relacionados con actividades matemáticas universales como: medir, contar y diseñar (Bishop, 1999). También, se logró conceptualizar una noción de SMA a partir de un ejercicio de reflexión conjunta respecto a lo que las sabedoras consideran como matemático y ancestral dentro del tejido, reconociendo en ellas, no solo la capacidad de ejecución de una práctica, sino también, y principalmente, la capacidad de reflexionar frente a esta. Así mismo, se consiguió describir el proceso de elaboración de algunos tejidos en lana de ovejo, descubriendo que para tejer se desarrollan 6 etapas, y se develó que para la comunidad de Caquiona esta práctica es sinónimo de vida y tradición, y que contribuye a su sobrevivencia y trascendencia en el tiempo.
What is the impact of an Abnormal pathogenic variant in COQ8A/CYP17A1 in pediatric patient - A case report
Authors: Alrowaished Bader Basem , Ali Elsayed , Alhejji Mohammed Saeed , Albohassen Mohammed Sami , Aldandan Ali Mohammed 
Published: August 2024

Background:
Primary coenzyme Q10 deficiency-4 (CoQ10D4) and isolated 17,20-lyase deficiency (ILD) are both distinct and rare congenital mutations. Mutations in the CoQ8A gene are rare and cause CoQ10D4. Patients with pathogenic mutations in the CoQ8A gene exhibit symptoms such as ataxic gait, dystonia, seizures, exercise intolerance, and cognitive disabilities.

Case presentation:
The presence of these two mutations in a patient is extremely rare. In this present case report, a 6-year-old identical twin female with CoQ10D4 and ILD began presenting with microcephaly with developmental delay at 4 years of age. She also had regression of milestones (loss of social interaction, grasp, visual interaction, and speech-babbling) without febrile seizures.

Conclusion:
This case emphasizes the need for further research to elucidate the underlying mechanisms and potential targeted therapies for these rare genetic disorders, emphasizing the importance of a multidisciplinary approach in providing comprehensive care for affected individuals.
Beyond the Joint: The Role of Central Nervous System Reorganizations in Chronic Musculoskeletal Disorders
Authors: Roy Jean-Sébastien , Bouyer Laurent J. , Langevin Pierre , Mercier Catherine 
Published: August 2024

To a large extent, management of musculoskeletal disorders has traditionally focused on structural dysfunctions found within the musculoskeletal system, mainly around the affected joint. While a structural-dysfunction approach may be effective for musculoskeletal conditions in some populations, especially in acute presentations, its effectiveness remains limited in patients with recurrent or chronic musculoskeletal pain. Numerous studies have shown that the human central nervous system can undergo plastic reorganizations following musculoskeletal disorders; however, they can be maladaptive and contribute to altered joint control and chronic pain. In this Viewpoint, the authors argue that to improve rehabilitation outcomes in patients with chronic musculoskeletal pain, a global view of the disorder that incorporates both central (neural) and peripheral (joint-level) changes is needed. The authors also discuss the challenge of evaluating and rehabilitating central changes and the need for large, high-level studies to evaluate approaches incorporating central and peripheral changes and emerging therapies. J Orthop Sports Phys Ther 2017;47(11):817–821. doi:10.2519/jospt.2017.0608
Identification of differentially expressed genes and screening for key genes involved in ovarian cancer prognosis: An integrated bioinformatics and network analysis approach
Authors: Niharika  , Roy Ankan , Patra Samir Kumar 
Published: August 2024

Objectives:
Ovaries are important and essential organs of animals in producing and releasing eggs. Ovarian cancer (OvCa) is one of the most prevalent lethal gynecological malignancies with a lack of distinct biomarkers. Advances in high-throughput genomic data and the continued refinement of bioinformatics tools enable the identification of potential biomarkers. Leveraging these insights, we can employ systems biology approaches to enhance the accuracy of diagnosis and prognosis.

Material and Methods:
A comparative analysis was conducted between normal and tumor samples, employing bioinformatics software and tools. Differential expression analysis utilized fold-change statistics, while DAVID 6.8 software was used to perform gene ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. The protein-protein interaction (PPI) network was constructed differentially expressed genes (DEGs) using Search Tool for the Retrieval of Interacting Genes database, and Cytoscape 3.9.1, along with its Molecular Complex Detection and CytoHubba plugins, facilitated network visualization, analysis, and module detection. Hub gene expression and overall survival were explored through the Kaplan–Meier plotter, while Gene Expression Profiling Interactive Analysis 2 analyzed the tumor stage of OvCa patients. Hub genes protein expression was analyzed using the human protein atlas database through immunostaining results. The NetworkAnalyst program and Cytoscape were employed to analyze and visualize the transcription factor-hub gene associations. Subsequently, single-nucleotide variation, methylation, and pathway activity of hub genes were examined. Validation of hub genes messenger RNA expression was done using quantitative real-time polymerase chain reaction analysis.

Results:
607 DEGs, including 248 upregulated and 359 downregulated genes, were identified. The top 20 candidate genes were screened out through PPI network analysis. We discovered that the genes BUB1 Mitotic Checkpoint Serine/Threonine Kinase B (BUB1B), Cyclin A2 (CCNA2), Mitotic Arrest Deficient 2 Like 1 (MAD2L1), Protein Regulator of Cytokinesis 1 (PRC1), Thyroid Hormone Receptor Interactor 13 (TRIP13), and ZW10 Interacting Kinetochore Protein (ZWINT) exhibited significant importance in OvCa prognosis.

Conclusion:
Six genes, BUB1B, CCNA2, MAD2L1, PRC1, TRIP13, and ZWINT (identified as functional hub genes), are probably playing tumor-promotive roles, except TRIP13. All genes product is functionally related to the cell cycle. These can be targeted in quest of potential therapeutics for OvCa treatment.
MASKER WAJAH DENGAN BAHAN AKTIF EKSTRAK CEMPAKA KUNING
Authors: Wulantresna Dewi , Zuhrotun Ade , Chaerunisaa Anis Yohana 
Published: August 2024

Sediaan kosmetika masker wajah yang mengandung bahan alami saat ini telah banyak beredar dipasaran, namun belum ada sediaan masker wajah dengan bahan aktif ekstrak bunga cempaka kuning. Adanya aktivitas antioksidan bunga cempaka kuning telah dilaporkan dari ekstrak dengan nilai IC50 5,12 µg/mL dan dari masker gel peel off yang mengandung ektrak bunga dengan nilai persen peredaman sebesar 51,75% mengandung 2 x IC50 (F2) dan 54,5% mengandung 3 x IC50 (F3). Namun sediaan masker gel tersebut belum diketahui keamanan dan kestabilan fisik sediaannya. Studi literatur ini bertujuan untuk melihat dan mengetahui potensi ekstrak cempaka kuning dalam pengembangan pada sediaan masker wajah dan beberapa pengujian kualitas dan kestabilan sediaan masker wajah. Metode dalam kajian literatur ini adalah pencarian artikel jurnal dan buku yang bersumber dari database PubMed, Sciencedirect dan Google Scholar. Hasil penelusuran pustaka diperoleh 99 artikel dipilih untuk skrining awal. Berdasarkan kajian literatur dapat disimpulkan bahwa ekstrak cempaka dapat memungkinkan untuk dikembangkan menjadi bentuk sediaan masker wajah, baik masker peel off atau sediaan masker lainnya.
Kata kunci: Antioksidan, ekstrak bunga cempaka kuning, masker wajah
Abstract
Face mask as cosmetic preparations containing natural ingredients are currently widely distributed in the market, but there is no face mask preparation with the active ingredient yellow champaka flower extract. Antioxidant activity of yellow champaka flower has been reported from extract with an IC50 value of 5.12 µg/mL and from a peel off gel mask containing this extract with a percent inhibition of 51.75% containing 2xIC50 (F2) and 54.5% containing 3xIC50 (F3). However, this gel mask preparation is not yet known for its safety and physical stability. This literature study aims to know the potential of yellow champaka extract in the development of facial mask preparations and its several quality and stability tests. The method in this literature review is the search for journal articles and books sourced from the PubMed, Sciencedirect and Google Scholar databases. The results of the literature search obtained 99 articles selected for initial screening. Based on the literature review, it can be concluded that champaka extract can allow it to be developed into a face mask dosage form, either a peel off mask or other mask preparations.
Keywords: Antioxidant, yellow champaka flower extract, face mask