bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silencing of cytochrome P450 CYP6B6 gene of cotton bollworm (Helicoverpa armigera) by RNAi
Authors: Zhang X. , Liu X. , Ma J. , Zhao J. 
Published: April 2013

AbstractRNA interference (RNAi) induced through double-stranded RNA (dsRNA) has been used widely to study gene function in insects. In this paper we demonstrate the efficacy of RNAi in the cotton bollworm, Helicoverpa armigera. Using CYP6B6 as the target gene, which is expressed in the fat baby and midgut of the lepidopteran pest H. armigera, we constructed the vector which expressed dsRNA of CYP6B6. Northern blot analysis showed that dsRNA expressed in the Escherichia coli (HT115) was target gene. The results also showed that the gene expression level and protein expression level of H. armigera larvae fed with dsRNA expressed by E. coli were significantly lower than those of all controls, but the gene expression level was more obvious than that at the protein level; significant lethality differences were also found between HT115 bacteria containing L4440-dsC1 treatment and HT115 bacteria containing L4440 vector or CK (ddH2O) in instar larvae on 4 day when continuous feeding, 32.45% mortality was recorded in the group of feeding HT115 bacteria containing L4440-dsC1 on 10 day. Our results suggest that the RNAi pathway can be exploited to control insect pests.
Emotion and Strategy in the Korean War
Authors: Mercer Jonathan 
Published: April 2013

AbstractWhat makes a diplomatic or military signal credible? In strategic settings where deception is possible, rational actors' interpretations rely on their beliefs, intuition, and imagination—they rely on emotion. Two properties of emotion—as an assimilation mechanism and its use as evidence—are key to addressing four strategic problems. First, emotion explains why actors worry needlessly about their reputations. Second, emotion is important to understanding costly signals. Third, emotion explains radical changes in preferences. Fourth, emotion sharpens understanding of strategic problems without being self-invalidating: common knowledge of emotion's effects do not always change those effects. Understanding how rational actors think requires turning to emotion. Evidence from the Korean War captures strengths and weaknesses of competing perspectives.
Theorizing Agency in Hobbes's Wake: The Rational Actor, the Self, or the Speaking Subject?
Authors: Epstein Charlotte 
Published: April 2013

AbstractThe rationalist-constructivist divide that runs through the discipline of International Relations (IR) revolves around two figures of agency, the rational actor and the constructivist “self.” In this article I examine the models of agency that implicitly or explicitly underpin the study of international politics. I show how both notions of the rational actor and the constructivist self have remained wedded to individualist understandings of agency that were first incarnated in the discipline's self-understandings by Hobbes's natural individual. Despite its turn to social theory, this persistent individualism has hampered constructivism's ability to appraise the ways in which the actors and structures of international politics mutually constitute one another “all the way down.” My purpose is to lay the foundations for a nonindividualist, adequately relational, social theory of international politics. To this end I propose a third model of agency, Lacan's split speaking subject. Through a Lacanian reading of theLeviathan, I show how the speaking subject has in fact laid buried away in the discipline's Hobbesian legacy all along.
Delayed Ratification: The Domestic Fate of Bilateral Investment Treaties
Authors: Haftel Yoram Z. , Thompson Alexander 
Published: April 2013

AbstractSome treaties are signed and then ratified quickly while others languish in legal limbo, unratified by one or more parties. What explains this variation in the time between signature and ratification? The international relations literature has not taken the ratification stage seriously enough, despite its obvious importance from a legal and a political perspective. We offer a systematic study of this question in the context of bilateral investment treaties. We develop and test a set of theoretical propositions related to domestic-level constraints on the executive, the varying ability of governments to rationally anticipate ratification obstacles, and the bilateral relationship between treaty partners. We generally find support for these propositions but report some surprising findings as well. The article presents implications for investment agreements and treaty making more generally, and raises a number of issues for further study at the intersection of international politics and law.
A survey of entomopathogenic nematode species in continental Portugal
Authors: Valadas V. , Laranjo M. , Mota M. , Oliveira S. 
Published: April 2013

AbstractEntomopathogenic nematodes (EPN) are lethal parasites of insects, used as biocontrol agents. The objectives of this work were to survey the presence of EPN in continental Portugal and to characterize the different species. Of the 791 soil samples collected throughout continental Portugal, 53 were positive for EPN. Steinernema feltiae and Heterorhabditis bacteriophora were the two most abundant species. Analysis of EPN geographical distribution revealed an association between nematode species and vegetation type. Heterorhabditis bacteriophora was mostly found in the Alentejo region while S. feltiae was present in land occupied by agriculture with natural vegetation, broadleaved forest, mixed forest and transitional woodland-shrub, agro-forestry areas, complex cultivated patterns and non-irrigated arable land. Although no clear association was found between species and soil type, S. feltiae was typically recovered from cambisols and H. bacteriophora was more abundant in lithosols. Sequencing of the internal transcribed spacer (ITS) region indicated that S. feltiae was the most abundant species, followed by H. bacteriophora. Steinernema intermedium and S. kraussei were each isolated from one site and Steinernema sp. from two sites. Phylogenetic analyses of ITS, D2D3 expansion region of the 28S rRNA gene, as well as mitochondrial cytochrome c oxidase subunit I (COXI) and cytochrome b (cytb) genes, was performed to evaluate the genetic diversity of S. feltiae and H. bacteriophora. No significant genetic diversity was found among H. bacteriophora isolates. However, COXI seems to be the best marker to study genetic diversity of S. feltiae. This survey contributes to the understanding of EPN distribution in Europe.
Threatened predator on the equator: multi-point abundance estimates of the tiger Panthera tigris in central Sumatra
Authors: Sunarto  , Kelly Marcella J. , Klenzendorf Sybille , Vaughan Michael R. , Zulfahmi  , Hutajulu M.B. , Parakkasi Karmila 
Published: April 2013

AbstractInformation on spatial and temporal variation in abundance is crucial for effective management of wildlife. Yet abundance estimates for the Critically Endangered Sumatran tiger Panthera tigris sumatrae are lacking from Riau, the province historically believed to hold the largest percentage of this subspecies. Recently, this area has had one of the highest global rates of deforestation. Using camera traps we investigated tiger abundance across peatland, flat lowland, and hilly lowland forest types in the province, and over time, in the newly established Tesso Nilo National Park, central Sumatra. We estimated densities using spatially explicit capture–recapture, calculated with DENSITY, and traditional capture–recapture models, calculated with CAPTURE. With spatially explicit capture–recapture the lowest tiger density (0.34 ± SE 0.24 per 100 km2) was estimated in the hilly lowland forest of Rimbang Baling and the highest (0.87 ± SE 0.33 per 100 km2) in the flat lowland forest of the Park. Repeated surveys in the Park documented densities of 0.63 ± SE 0.28 in 2005 to 0.87 ± SE 0.33 per 100 km2 in 2008. Compared to traditional capture–recapture the spatially explicit capture–recapture approach resulted in estimates 50% lower. Estimates of tiger density from this study were lower than most previous estimates in other parts of Sumatra. High levels of human activity in the area appear to limit tigers. The results of this study, which covered areas and habitat types not previously surveyed, are important for overall population estimates across the island, provide insight into the response of carnivores to habitat loss, and are relevant to the interventions needed to save the tiger.
Fishermen's perceptions of interactions between seabirds and artisanal fisheries in the Chonos archipelago, Chilean Patagonia
Authors: Suazo Cristián G. , Schlatter Roberto P. , Arriagada Aldo M. , Cabezas Luis A. , Ojeda Jaime 
Published: April 2013

AbstractInteractions between seabirds and commercial fishing activities have been well documented but little information is available regarding the impacts of more traditional fishing practices on seabird populations. We interviewed fishermen, administered questionnaires, and made field-based observations to determine the extent to which artisanal fisheries interact with and affect seabirds in the fjords and channels of the Chonos archipelago in southern Chile. Our surveys indicated a positive perception of seabirds as useful indicators of marine productivity and in their role scavenging fish waste and discards associated with fishing operations. However, the surveys also revealed that fishermen routinely establish seasonal camps for collecting seabird eggs and adults for food or bait and introduce feral predators to seabird breeding colonies on islands. Understanding the traditional practices of fishermen is critical for the future of community-based conservation of the region's marine resources and biodiversity.
Conservation status of the narrow endemic gypsophile Ononis tridentata subsp. crassifolia in southern Spain: effects of habitat disturbance
Authors: Ballesteros Miguel , Foronda Ana , Cañadas Eva María , Peñas Julio , Lorite Juan 
Published: April 2013

AbstractOnonis tridentata L. subsp. crassifolia (Leguminosae) is a narrow endemic plant restricted to gypsum outcrops in south-east Spain. Its habitat and populations are currently threatened by anthropogenic disturbance. Because of the paucity of information concerning its distribution, abundance and response to disturbance, we assessed its status and evaluated the impacts of quarrying, ploughing, grazing and afforestation. Distribution and population size were estimated by field surveys, censuses and mapping. We measured cover, plant volume, fruit and seed production, seed predation and seedling recruitment to asses any effects of disturbance. The species’ area of occupancy is 1.6 km2 and its extent of occurrence 337 km2, in 29 habitat patches and 16 populations between 705 and 1,125 m altitude, and its population size was estimated to be 531,605. Quarrying, ploughing, overgrazing and afforestation negatively affected the species, in this order. We recommend this subspecies be categorized as Vulnerable on the IUCN Red List. A species recovery plan is required, and the ecological restoration of altered areas would mitigate negative effects on the species and improve the overall conservation of gypsum habitats.
Change in cannabis use, clinical symptoms and social functioning among patients with first-episode psychosis: a 5-year follow-up study of patients in the OPUS trial
Authors: Clausen L. , Hjorthøj C. R. , Thorup A. , Jeppesen P. , Petersen L. , Bertelsen M. , Nordentoft M. 
Published: April 2013

BackgroundSeveral studies indicate that cannabis use among patients with psychotic disorders is associated with worse outcome, but only a few studies have controlled for baseline condition and medication.MethodAt 5-year follow-up, interviews were carried out with 314 first-episode psychosis patients included in the OPUS trial. The patients included were in the age range of 18 to 45 years old and 59% were male. Cannabis use was extracted from the Schedule for Clinical Assessment in Neuropsychiatry. At follow-up, the patients were divided into different groups according to the variable cannabis use: abstainers, stoppers, starters and continuers. Psychotic, negative and disorganized dimensions (ranging from zero to five) were calculated for each of the four groups based on the Schedule for the Assessment of Positive and Negative Symptoms in Schizophrenia.ResultsCannabis users were younger (24.6 years v. 27.4 years, p < 0.001) and had a lower level of education. At the 5-year follow-up, users of cannabis had higher scores on the psychotic dimension [difference 0.97, 95% confidence interval (CI) 0.41–1.53, p = 0.001] and lower levels of the Global Assessment of Functioning (difference 8.26, 95% CI 2.13–14.39, p = 0.01). Those who stopped using cannabis between entry and 5-year follow-up had a significantly lower level of psychotic symptoms at 5-year follow-up even after controlling for baseline level of psychotic symptoms and for insufficient antipsychotic medication (adjusted difference in psychotic dimension –1.04, 95% CI –1.77 to –0.31, p = 0.006).ConclusionsContinuous cannabis use was associated with higher levels of psychotic symptoms after 5 years, and this association was only partly explained by insufficient antipsychotic medication.
Lifetime prevalence and co-morbidity of externalizing disorders and depression in prospective assessment
Authors: Hamdi N. R. , Iacono W. G. 
Published: April 2013

BackgroundEpidemiological research is believed to underestimate the lifetime prevalence of mental illness due to recall failure and a lack of rapport between researchers and participants.MethodIn this prospective study, we examined lifetime prevalence and co-morbidity rates of substance use disorders, antisocial personality disorder (ASPD) and major depressive disorder (MDD) in a representative, statewide Minnesota sample (n = 1252) assessed four times between the ages of 17 and 29 years with very low attrition.ResultsLifetime prevalence rates of all disorders more than doubled between the ages of 17 and 29 years in both men and women, and our prospective rates at the age of 29 years were consistently higher than rates from leading epidemiological surveys. Although there was some variation, the general trend was for lifetime co-morbidity to increase between the ages of 17 and 29 years, and this trend was significant for MDD–alcohol dependence, MDD–nicotine dependence, and ASPD–nicotine dependence.ConclusionsOverall, our results show that emerging adulthood is a high-risk period for the development of mental illness, with increases in the lifetime prevalence and co-morbidity of mental disorders during this time. More than a quarter of individuals had met criteria for MDD and over a fifth had experienced alcohol dependence by the age of 29 years, indicating that mental illness is more common than is estimated in cross-sectional mental health surveys. These findings have important implications for the measurement of economic burden, resource allocation toward mental health services and research, advocacy organizations for the mentally ill, and etiological theories of mental disorders.
Antimicrobial resistance patterns and genotypes ofSalmonella entericaserovar Hadar strains associated with human infections in Switzerland, 2005–2010
Authors: CERNELA N. , NÜESCH-INDERBINEN M. , HÄCHLER H. , STEPHAN R. 
Published: April 2013

SUMMARYSalmonellaHadar ranks in the top ten serovars reported from humans in Switzerland. In this study, all 64S.Hadar strains isolated from different patients from 2005 to 2010 in Switzerland were characterized by (i) assessing phenotypic antimicrobial resistance profiles using the disk diffusion method and (ii) by genotyping using pulsed-field gel electrophoresis (PFGE) in order to evaluate the relationship of the strains. The annual incidences varied between 0·32/100 000 in 2005 (highest incidence) and 0·065/100 000 in 2007 (lowest incidence). In total 71·8% of the isolates were resistant to nalidixic acid. Although 40·6% of the strains were resistant to theβ-lactam antibiotic ampicillin, they remained susceptible to the third-generation cephalosporin cefotaxime. Genotyping revealed a primary cluster consisting of 42 strains, sharing a similarity of >92%, with a subcluster of 18 strains with indistinguishable patterns. Resistance profiles allowed further differentiation within this subcluster providing a link of two strains to an outbreak in Spain.
Marginal marine depositional setting and correlation of the Devonian Sperm Bluff Formation (Taylor Group), southern Victoria Land, Antarctica
Authors: Savage Jeni E. , Bradshaw Margaret A. , Bassett Kari N. 
Published: April 2013

AbstractAnalysis of conglomerates and sandstones of the Sperm Bluff Formation at the base of the Taylor Group (Devonian) between the Mackay Glacier and Bull Pass provides new insights into the nature of initial coarse-grained deposition on basement along the northern side of the McMurdo sedimentary basin. Six lithofacies are recognized in the Sperm Bluff Formation: conglomerate lithofacies, pebbly sandstone lithofacies, cross-bedded sandstone lithofacies, low-angle cross-stratified sandstone lithofacies, bioturbated sandstone lithofacies and interbedded siltstone/sandstone lithofacies. Sedimentary environments ranged from wave-dominated delta, estuary or lagoon to shoreface and inner shelf. The assemblage is thought to reflect changes in sea level. Rhyolite is the most abundant clast type in the coarse lithofacies, but is unknown in outcrop in southern Victoria Land. The rhyolites correlate in age and geochemistry with Cambrian granites in the basement. Coarse beds also contain numerous quartzite clasts, probably derived from the late Precambrian Skelton Group. Palaeocurrents on Mount Suess indicate a strong unimodal flow to the west, but other sites show polymodal palaeoflow. The Sperm Bluff Formation is correlated with Terra Cotta Siltstone, New Mountain Sandstone and Altar Mountain formations based on the lithology of sandstones and their ichnology. A northward onlap during the Early Devonian is indicated.
Proteomic analysis of the enhancement of seed vigour in osmoprimed alfalfa seeds germinated under salinity stress
Authors: Yacoubi Rafika , Job Claudette , Belghazi Maya , Chaibi Wided , Job Dominique 
Published: April 2013

AbstractAlfalfa (Medicago sativaL.) yield is severely compromised by soil salinity, especially at the level of seedling establishment. This question was addressed by proteomics to decipher whether specific changes in protein accumulation correlate with germination performance of alfalfa seeds submitted to a salinity stress as obtained by imbibing seeds in the presence of NaCl. This study used alfalfa seeds submitted to an osmopriming invigoration treatment that proved very efficient in counteracting the negative effect of salinity stress on germination performance. Comparative proteomic analyses disclosed 94 proteins commonly characterizing the response of both the untreated control and osmoprimed seeds to the experimental salinity stress. Remarkably, many of them, representing 84 proteins, showed contrasting accumulation patterns when comparing the untreated control and osmoprimed seeds submitted to the same salt stress. Thus numerous changes observed in the proteome of the untreated control seeds imbibed in the presence of salt, and presumably accounting for the loss in seed vigour associated with salinity stress, can be substantially reversed in osmoprimed seeds undergoing this stress. These data therefore provide a biochemical understanding of the increase in seed vigour generally observed with primed seeds.
N-acetyl-cysteine prevents toxic oxidative effects induced by IFN-α in human neurons
Authors: Alboni Silvia , Gibellini Lara , Montanari Claudia , Benatti Cristina , Benatti Stefania , Tascedda Fabio , Brunello Nicoletta , Cossarizza Andrea , Pariante Carmine M. 
Published: April 2013

Abstract
Currently IFN-α is widely used for effective treatment of viral infections and several malignancies. However, IFN-α can cause neuropsychiatric disturbances and mental impairments, including fatigue, insomnia, depression, irritability and cognitive deficits. Molecular and cellular mechanisms leading to such side-effects are still poorly understood. Neurons seem to be an important target in mediating cellular effects induced by exposure to this cytokine, but so far little is known about IFN-α-induced effects on these cells. We have investigated the ability of IFN-α (2–100 ng/ml) to induce damage and toxicity to the human neuroblastoma SH-SY5Y cell line, commonly used for studying such phenomena, and the mechanisms underlying these effects. After 24 h treatment, IFN-α increased mitochondrial activity, whereas cell density was reduced in a dose- and time-dependent manner. This effect did not depend on reduced cell proliferation, but rather the activation of apoptosis, as revealed by an increased Bax:Bcl-2 mRNA ratio after 72-h IFN-α exposure. At this time-point, IFN-α also reduced the expression of the brain-derived neurotrophic factor gene, and induced an increase in reactive oxygen species (ROS). A co-treatment with N-acetyl-cysteine (NAC; 5 mm), a potent antioxidant and mitochondrial modulator, was able to counteract all of these IFN-α-induced effects. These findings demonstrated that IFN-α induces neurotoxicity and apoptosis that is, in part, very likely due to mitochondrial damages and production of ROS. We suggest that NAC, already tested for the treatment of psychiatric disorders, may be useful to prevent IFN-α-induced central side-effects in a safe and effective way.
Xentry, a new class of cell-penetrating peptide uniquely equipped for delivery of drugs
Authors: Montrose Kristopher , Yang Yi , Sun Xueying , Wiles Siouxsie , Krissansen Geoffrey W. 
Published: April 2013

AbstractHere we describe an entirely new class of cell-penetrating peptide (CPP) represented by the short peptide Xentry (LCLRPVG) derived from an N-terminal region of the X-protein of the hepatitis B virus. Xentry permeates adherent cells using syndecan-4 as a portal for entry and is uniquely restricted from entering syndecan-deficient, non-adherent cells, such as resting blood cells. Intravenous injection of Xentry alone or conjugated to β-galactosidase led to its delivery to most tissues in mice, except circulating blood cells. There was a predilection for uptake by epithelia. Anti-B-raf antibodies and siRNAs linked to Xentry were capable of killing B-raf-dependent melanoma cells. Xentry represents a new class of CPP with properties that are potentially advantageous for life science and therapeutic applications.