bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel growth-promoting dark septate endophytic fungus improved drought tolerance in blueberries by modulating phytohormones and non-structural carbohydrates
Authors: Su Hongyan , Guo Yingtian , Gu Liang , Shi Xiaomeng , Zhou Yangyan , Wu Fanlin , Wang Lei 
Published: August 2024

Abstract
Drought is a significant global issue affecting agricultural production, and the utilization of beneficial rhizosphere microorganisms is one of the effective ways to increase the productivity of crops and forest under drought. In this study, we characterized a novel growth-promoting dark septate endophytes (DSE) fungus R16 (Dothideomycetes sp.) derived from blueberry roots. Hyphae or microsclerotia were visible within the epidermal or cortical cells of R16-colonized blueberry roots, which was consistent with the typical characteristics of DSE fungi. Inoculation with R16 promoted the growth of blueberry seedlings, and the advantage over the control group was more significant under PEG-induced drought. Comparison of physiological indicators related to drought resistance between the inoculated and control groups was performed on the potted blueberry plants, including the chlorophyll content, net photosynthetic rate, root activities, malondialdehyde and H2O2 content, which indicated that R16 colonization mitigated drought injury in blueberry plants. We further analyzed the effects of R16 on phytohormones and non-structural carbohydrates (NSCs) to explore the mechanism of increased drought tolerance by R16 in blueberry seedlings. The results showed that except for the gibberellin content, indole-3-acetic acid, zeatin and abscisic acid varied significantly between the inoculated and control groups. Sucrose phosphate synthase and sorbitol-6-phosphate dehydrogenase activities in mature leaves, the key enzymes responsible for sucrose and sorbitol synthesis, respectively, as well as sorbitol dehydrogenase, sucrose synthase, cell wall invertase, hexokinase and fructokinase in roots, the key enzymes involved in the NSCs metabolism, showed significant differences between the inoculated and control groups before and after drought treatment. These results suggested that the positive effects of R16 colonization on the drought tolerance of blueberry seedlings are partially attributable to the regulation of phytohormone and sugar metabolism. This study provided valuable information for the research on the interaction between DSE fungi and host plants as well as the application of DSE preparations in agriculture.
Loss of periostin function impairs ligament fibroblast activity and facilitates ROS‐mediated cellular senescence
Authors: Rai Muhammad Farooq , Duan Xin , Yan Mingming , Brophy Robert H. , Cai Lei 
Published: August 2024

AbstractAnterior cruciate ligament (ACL) injuries pose a significant challenge due to their limited healing potential, often resulting in premature arthritis. The factors and mechanisms contributing to this inadequate healing process remain elusive. During the acute phase of injury, ACL tissues express elevated periostin levels that decline over time. The functional significance of periostin in ligament biology remains understudied. In this study, we investigated the functional and mechanistic implications of periostin deficiency on ACL biology, utilizing ligament fibroblasts derived from patients and a murine model of ACL rupture. Our investigations unveiled that periostin knockdown compromised fibroblast growth characteristics, hindered the egress of progenitor cells from explants, and arrested cell‐cycle progression, resulting in the accumulation of cells in the G0/G1 phase and moderate apoptosis. Concurrently, a significant reduction in the expression of cell‐cycle and matrix‐related genes was observed. Moreover, periostin deficiency triggered apoptosis through STAT3Y705/p38MAPK signaling and induced cellular senescence through increased production of reactive oxygen species (ROS). Mechanistically, inhibition of ROS production mitigated senescence in these cells. Notably, in vivo data revealed that ACL in Postn−/− mice exhibited a higher tearing frequency than wild‐type mice under equivalent loading conditions. Furthermore, injured ACL with silenced periostin expression, achieved through nanoparticle‐siRNA complex delivery, displayed an elevated propensity for apoptosis and senescence compared to intact ACL in C57BL/6 mice. Together, our findings underscore the pivotal role of periostin in ACL health, injury, and potential for healing.
Silicosis
Authors: Cena Ashley C. , Cena Lorenzo G. 
Published: August 2024

ABSTRACT
Silicosis typically has been classified as a chronic disease that develops after at least 10 years of exposure to silica dust, and often is associated with miners and stone workers. As industries have changed over time, other types of workers (including those in artificial stonework, jewelry polishing, and denim production) have become exposed to high levels of silica, leading to the development of acute and accelerated silicosis. Acute silicosis can develop in as little as a few months, and accelerated silicosis can develop in as little as 2 years. No cure exists for any form of silicosis, and lung transplantation is the only lifesaving treatment. Primary care clinicians must understand when patients are at risk for developing silicosis and not assume that a short time of exposure precludes the development of silicosis.
Development of Postsurgical Pyoderma Gangrenosum with New Keloid after Keloid Resection
Authors: Saijo Yusaku , Kuwahara Hiroaki , Ito Keigo , Ogawa Rei , Akaishi Satoshi 
Published: August 2024

ABSTRACTKeloids are a dermal fibroproliferative disorder and can arise from trauma, acne, vaccination, and herpes zoster. Pyoderma gangrenosum (PG) is a painful ulcerative skin disorder that is associated with neutrophilic dysfunction. However, the pathophysiologies of keloids and PG are not fully understood. The authors encountered an unusual case of a 24-year-old woman who presented with an anterior chest keloid that bore an ulcer. The keloid was resected along with the ulcer, and histology revealed the ulcer to be a neutrophilic PG ulcer. A year after surgery, another ulcer developed in the scar. The ulcer met the PARACELSUS criteria of a postsurgical PG ulcer. After treatment with systemic prednisone and adalimumab for 250 days, the ulcer re-epithelialized. However, relapsed keloids were then observed at the PG site. Corticosteroid taping may be the safest therapy for patients with a history of PG. Conversely, if there is suspicion that a patient is prone to keloid development, diagnostic biopsies and surgical management of PG ulcers should be avoided or conducted with care.
Wound Itch: An Update
Authors: Paul Julia C. 
Published: August 2024

ABSTRACT

GENERAL PURPOSE
To present current evidence regarding the physiology, assessment, and management of wound itch.

TARGET AUDIENCE
This continuing education activity is intended for physicians, physician assistants, nurse practitioners, and registered nurses with an interest in skin and wound care.

LEARNING OBJECTIVES/OUTCOMES
After participating in this educational activity, the participant will:
1. Summarize current understanding of itch physiology.
2. Apply current evidence to the management of wound itch.

Background
Itch is a symptom that can be experienced by persons with open wounds. Whereas advances are being made in the understanding and management of itch, the molecular and cellular physiology of itch is still not fully understood.

Objective
To summarize recent literature to determine what developments have been made in terms of the physiology, assessment, and management of wound itch.

Data Sources
The literature was searched in PubMed and CINAHL Complete databases, limited to the last 5 years and articles in English.

Study Selection
Articles were selected based on reference to wound-related itch. The chosen articles and their references were thoroughly reviewed. Additional relevant studies were found in database alerts including Google Scholar and JAMA Online First: Medical News and in searching reference lists of associated articles.

Data Extraction
In addition to article author, title, and year of publication, extracted data included article type, purpose, results, recommendations, and limitations.

Data Synthesis
Findings related to wound-related itch, postburn itch, venous ulcers, itch physiology, itch assessment, and itch interventions are described.

Conclusion
The consensus is that wound-related itch is underevaluated and undertreated. The physiology of itch is not completely understood, and the effectiveness of treatments for wound itch is not known. Further studies with larger samples need to be undertaken to increase the understanding of wound-related itch; its etiology, physiology, and assessment; and treatment effectiveness.
Chronic Wound Caused by Lymphorrhea after Skin Abscess Drainage on Lower Extremity: A Case Report
Authors: Tian Jin-yi 
Published: August 2024

ABSTRACT
Surgical operations such as lymph node dissection may inadvertently damage the lymphatic system and lead to lymphorrhea. Excessive lymphatic exudation can cause a chronic wound. However, for surgery that does not involve the lymphatic system, lymphorrhea is a rare postoperative complication. This case report describes a 38-year-old patient who presented with lymphatic cutaneous leakage after incision and drainage of a skin abscess on the lower extremity. Persistent lymphorrhea increased the amount of wound exudation, which affected wound healing. After treatment of the lymphorrhea, the lower extremity wound healed completely within 4 weeks and did not result in lymphedema. Ligation of lymphatic vessels is a simple and easily performed treatment method for lymphatic leakage.
Systemic inflammation and menopausal symptomatology: insights from postmenopausal women
Authors: Korpe Busra , Kose Caner , Keskin Huseyin Levent 
Published: August 2024

Abstract

Objective
This study aims to investigate the association between systemic inflammation, measured by the systemic immune-inflammatory index (SII), and menopausal symptoms in postmenopausal women.

Methods
A cross-sectional study was conducted with 702 postmenopausal women recruited from a tertiary hospital. Participants completed the Menopause Rating Scale (MRS) to assess somatic, psychological, and urogenital symptoms. Blood samples were collected to calculate SII. Statistical analyses included correlation, univariate regression, and multivariate regression to determine the association between SII and menopausal symptoms.

Results
The most commonly reported symptoms were muscle and joint problems (75.9%), hot flashes and sweating (72.5%), sleeping problems (71.2%), and depressive mood (69.9%). Women with higher total and somatic subscale scores had significantly elevated SII levels compared with those with lower scores. SII was the most associated variable for high total MRS (odds ratio, 4.77; 95% CI, 3.36-6.78; P < 0.001) and somatic subscale scores (odds ratio, 6.32; 95% CI, 4.45-8.99; P < 0.001). Exercise and gravidity were negatively associated with high total MRS and somatic subscale scores. Conversely, no significant associations were observed between SII and the urogenital or psychological subscale scores.

Conclusion
Elevated SII levels were found associated with higher somatic subscale and total MRS scores, suggesting that inflammation may contribute to worsened somatic menopausal symptoms in this group. These findings suggest that exploring inflammatory pathways could be beneficial in managing menopausal symptoms and enhancing quality of life for postmenopausal women.
Clinical Presentation of Enterovirus D68 in a Swiss Pediatric University Center
Authors: Ott Chantal , Dutilh Gilles , Reist Josiane , Bingisser Roland , Egli Adrian , Heininger Ulrich 
Published: August 2024

Background:
Enterovirus D68 (EV-D68) is responsible for millions of infections. In the last decade, there has been an increase in the number of children requiring hospital or critical care admission due to severe respiratory illness. Nevertheless, the epidemiological and clinical importance of EV-D68 infections remains unclear.

Objective:
We aimed to determine the local prevalence of EV-D68 infection in pediatric patients and to characterize its clinical presentation and disease burden compared with non-EV-D68 enterovirus and human rhinovirus (RV) infections.

Study design:
We performed a retrospective single-center study of children presenting with respiratory symptoms and positive respiratory panel polymerase chain reaction for EV/RV from November 2018 to December 2019. We tested EV/RV positive specimens with an EV-D68-specific polymerase chain reaction to discriminate EV-D68, non-EV-D68 and RV and compared their respective clinical presentation, outcomes and treatment.

Results:
We identified 224 patients (median age 21 months), of which 16 (7%) were EV-D68 positive. They presented with cough (88%), wheezing (62%) and dyspnea (75%). EV-D68 infection had an odds ratio regarding pediatric respiratory severity-score of 11.6 relative to non-EV-D68 [confidence intervals (CI): 3.51–41.14], and of 9.9 (CI: 3.75–27.95) relative to RV. The fitted logistic regression showed that the odds of intensive care were 5 times more likely with EV-D68 than RV infection (CI: 1.32–19.28; P = 0.001). Patients with EV-D68 infections were more likely to receive medical support in the form of supplementary oxygen, antibiotics and steroids.

Conclusions:
EV-D68 infection is associated with higher morbidity and a higher likelihood of intensive care treatment than non-EV-D68 and RV infections.
Burnout Reduction Strategies Using Mobile-Based Applications: The Creation and Development of the SANE Well App
Authors: Williams Deborah , Bouchard Lindsay , Giraldo Isabel , Carstensen Gary , Kiser Lisa , Acosta Lauren , Buxbaum Susan , Wong Ava , Taren Douglas 
Published: August 2024

ABSTRACT

Introduction
Sexual assault nurse examiners (SANEs) provide specialized medical forensic care to survivors of sexual assault, often working in stressful conditions, with routine exposure to the traumatic experiences of their patients. SANEs experience high levels of both vicarious trauma and burnout.

Purpose
The purpose of this article was to describe the development of a SANE support application, accessible to all SANEs, that utilizes self-care and positive coping strategies to enhance SANE resilience and thereby reduce burnout.

Methods
The conceptual framework for the SANE support application was psychological first aid, an evidence-informed, manualized intervention to assist individuals in the immediate aftermath of exposure to potentially traumatic events. Focus groups with SANEs informed the content of the application named “SANE Well.”

Result
SANE Well delivers these helpful coping elements within an easy-to-use, mobile platform, at no cost. User testing during two SANE clinical skills laboratory trainings revealed the app had smooth and efficient functionality and meets its intended goals without unnecessary complexities.

Conclusion
There is an urgent need for SANEs to protect their well-being while they care for individuals who have experienced sexual assault. The SANE Well application offers a novel way to provide and encourage positive coping strategies for nurses to reduce burnout.
The Impact of Transitional Shock on Newer Nurses in a Contemporary Healthcare Environment
Authors: Holtz Heidi , McQueen Amy , Weissinger Guy , Alderfer Mary , Swavely Deborah , Sledge Jennifer A. , Yu Lihua , Pohlman Megan , Adil Thomas , Mugoya Rosemary , Minchhoff Robin , Rushton Cynda 
Published: August 2024

OBJECTIVE
The aim of this study was to understand newer nurses' perception and expectations of their work environment, professional and institutional satisfaction, and motivating/decision-making factors around nursing and intent to leave their positions.

BACKGROUND
Studies have shown newly graduated nurses face many challenges transitioning from a student to an independently practicing nurse. The COVID pandemic complicated this transition and created new stressors resulting in discouragement and turnover for newer nurses.

METHODS
This study used a qualitative approach through purposive sampling, semistructured qualitative interviews, and content analytic techniques. Transitional Shock Theory was used as the theoretical framework.

RESULTS
Four themes emerged from the study: 1) confronting the reality; 2) betrayal/mistrust; 3) relational integrity; and 4) enablers of clinical competence.

CONCLUSIONS
The findings will help nurse leaders understand the importance of organizational-level supports to improve the transition of newer nurses in typical periods and in times of additional stress.
Critical elements of international academic partnerships in orthopaedic surgery: a modified Delphi approach
Authors: Flores Michael J. , MacKechnie Madeline C. , Rodarte Patricia , O'Marr Jamieson M. , Brown Kelsey E. , Shearer David W. , Miclau Theodore ,   COACT Delphi Study Group
Published: August 2024

Abstract

Background:
Despite the recent emphasis on promoting international collaborations within orthopaedic surgery, criteria for determining the strengths of such partnerships has not been established. The purpose of this study was to evaluate orthopaedic experts' perceptions of the most valuable characteristics of international academic partnerships.

Methods:
This study was conducted using a modified Delphi methodology. Experts were identified through the Consortium of Orthopaedic Academic Traumatologists (COACT). Responses were collected from February to September 2022. Three rounds of surveys listing possible topics on a 5-point Likert scale were used to develop consensus among a group of experts. Consensus criteria for topic inclusion in the final scale was determined as a rating of “strongly agree” or “agree” by ≥70% of the participants in the third survey.

Results:
The Round 1 survey was distributed to 96 invited participants within the COACT network, of which 50 experts (52.1%) completed the first survey. Consensus was reached on 54 topics organized into the following 5 categories: Research, Advocacy/Leadership, Training/Surgical Skills, Education/Knowledge Exchange, and Sustainability and Safety (RATES Criteria).

Conclusions:
Determining the most valuable characteristics of successful international academic partnerships can lead to more sustainable, mutually beneficial collaborations. The criteria identified in this study can provide the foundation for developing new partnerships and assessing existing ones.
Traumatic Injuries From Low Speed Vehicles and Golf Carts
Authors: Barber Kristina , Nikolis Andrea , Machek Megan , Akuthota Venu , Odom Nathan 
Published: August 2024

Abstract
With increasing use of golf carts and low speed vehicles for recreational, work-related, and commuting purposes, the number of related injuries is also on the rise. The body of literature describing these injuries is informative but limited. From this scoping review, we found that low speed vehicle–related injuries preferentially affect children, older adults, and males. Common mechanisms of injury include ejection and collision. Associated alcohol consumption is common and typically related to increasing severity of injury. Various injuries may result from low speed vehicles as described in the review, including soft tissue injuries, orthopedic injuries, and traumatic brain injury. Description of trends and classifications of injury will hopefully improve reporting and ability to make recommendations on safe use and regulation.
THE ROLE OF TRANEXAMIC ACID IN POSTPARTUM HEMORRHAGE: A NARRATIVE REVIEW
Authors: Larson Nicholas J. , Mergoum Anaas Moncef , Dries David J. , Cook Alan , Blondeau Benoit , Rogers Frederick B. 
Published: August 2024

ABSTRACT
Postpartum hemorrhage is the leading cause of preventable maternal illness and death globally and carries a disproportionately high burden of mortality in low- to middle-income countries. Tranexamic acid, an antifibrinolytic drug, has been widely adopted to control bleeding in trauma and other surgical conditions. Within the last decade, the World Health Organization updated their guidelines for the treatment of postpartum hemorrhage to include the use of tranexamic acid in all cases of postpartum hemorrhage. However, despite these guidelines and the proven utility of tranexamic acid to treat postpartum hemorrhage, widespread adoption of tranexamic acid into global standards of care across professional organizations has not been achieved. It is important for healthcare providers to understand the etiologies of postpartum hemorrhage, the mechanism of action and adverse effect profile of tranexamic acid, and the available literature regarding the use of tranexamic acid to prevent and treat postpartum hemorrhage to provide the best care for the pregnant patient.
The Role of Coronavirus Spike Protein in Inducing Optic Neuritis in Mice: Parallels to the SARS-CoV-2 Virus
Authors: Kasle Grishma , Das Sarma Jayasri 
Published: August 2024

Background:Optic neuritis (ON), one of the clinical manifestations of the human neurological disease multiple sclerosis (MS), was also reported in patients with COVID-19 infection, highlighting one potential neurological manifestation of SARS-CoV-2. However, the mechanism of ON in these patients is poorly understood.Evidence Acquisition:Insight may be gained by studying the neurotropic mouse hepatitis virus (MHV-A59), a β-coronavirus that belongs to the same family as SARS-CoV-2.Results:Mouse hepatitis virus-A59, or its isogenic spike protein recombinant strains, inoculation in mice provides an important experimental model to understand underpinning mechanisms of neuroinflammatory demyelination in association with acute stage optic nerve inflammation and chronic stage optic nerve demyelination concurrent with axonal loss. Spike is a surface protein that mediates viral binding and entry into host cells, as well as cell–cell fusion and viral spread. Studies have implicated spike-mediated mechanisms of virus-induced neuroinflammatory demyelination by comparing naturally occurring demyelinating (DM) and nondemyelinating (NDM) MHV strains.Conclusions:Here, we summarize findings in MHV-induced experimental ON and myelitis, using natural DM and NDM strains as well as engineered recombinant strains of MHV to understand the role of spike protein in inducing ON and demyelinating disease pathology. Potential parallels in human coronavirus-mediated ON and demyelination, and insight into potential therapeutic strategies, are discussed.