bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ultrasound‐guided Ischial Bursa Injection: Technique and Positioning Considerations
Authors: Wisniewski Steve J. , Hurdle Mark , Erickson Jason M. , Finnoff Jonathan T. , Smith Jay 
Published: August 2013

ObjectivesTo 1) describe and validate an ultrasound‐guided ischial bursa injection technique in an unembalmed cadaveric model and 2) to compare the distance between the ischial tuberosity and the sciatic nerve in a hip neutral versus 90° flexed hip position in asymptomatic volunteers.DesignThe first part was a single‐blind prospective study. The second part was a prospective cohort study.SettingAn academic institution procedural skills laboratory and outpatient clinic.ParticipantsThe first part of the study involved 1 cadaveric specimen. The second part of the study involved 20 asymptomatic subjects. The mean age of the subjects was 28 years, and the mean (standard deviation) body mass index was 23.2 ± 2.8 kg/m2 (minimum, 18.3 kg/m2; maximum, 29.5 kg/m2).MethodsIn the first part of the study, a single operator completed bilateral ultrasound‐guided ischial bursa injections in an unembalmed cadaveric specimen by using diluted colored latex. In the second part of the study, ultrasound was used in 20 asymptomatic volunteer subjects (10 men and 10 women) to measure the distance from the lateral edge of the ischial tuberosity to the sciatic nerve.Main Outcome MeasurementsThe injections were graded for accuracy as follows: accurate (all injectate contained within the ischial bursa), accurate with overflow (injectate within the ischial bursa but also located in adjacent structures other than the needle track), or inaccurate (injectate not within the ischial bursa). The second part of the study measured the distance from the ischial tuberosity to the sciatic nerve with subjects in 2 different positions (prone and side lying with the tested hip flexed to 90°).ResultsPostinjection cadaveric dissections revealed that both ultrasound‐guided injections accurately placed liquid latex within the ischial bursae. There was no evidence of injury to surrounding neurovascular structures. Among asymptomatic volunteers, the average distance between the ischial tuberosity and the sciatic nerve increased from 28.4 mm (range, 20.5‐38.9 mm) in the neutral position to 41.9 mm (range, 30.9‐66.0 mm) with the hip flexed to 90° (average change, 13.5 mm away from the ischial tuberosity; P = .0001).ConclusionsUltrasound‐guided ischial bursa injections are technically feasible. Flexing the hip to 90° increases the distance between the ischial tuberosity and the sciatic nerve in asymptomatic volunteers, thus potentially resulting in a safer needle trajectory when ischial bursa injections are clinically indicated. Further investigation in clinical settings is warranted to validate these findings.
Medical Planning for Extended Remote Expeditions
Authors: Iserson Kenneth V. 
Published: August 2013

Remote extended expeditions often support scientific research and commercial resource exploration or extraction in hostile environments. Medical support for these expeditions is inherently complex and requires in-depth planning. To be successful, this planning must include substantial input from clinicians with experience in remote, emergency, and prehospital medicine and from personnel familiar with the proposed working environment. Using the guidelines discussed in this paper will help ensure that planners consider all necessary, medically relevant elements before launching an extended remote expedition. The 10 key elements of a workable remote healthcare system are to: Optimize workers’ fitness Anticipate treatable problems Stock appropriate medications Provide appropriate equipment Provide adequate logistical support Provide adequate medical communications Know the environmental limitations on patient access and evacuation Use qualified providers Arrange for knowledgeable and timely consultations Establish and distribute rational administrative rules Planners using these guidelines may better be able to generate a strategy that optimizes the participants’ health benefits, the expedition’s productivity, and the expedition sponsor’s cost savings.
FRACTIONAL POWERS OF DEHN TWISTS ABOUT NONSEPARATING CURVES
Authors: RAJEEVSARATHY KASHYAP 
Published: September 2013

AbstractLet Sg be a closed orientable surface of genus g ≥ 2 and C a simple closed nonseparating curve in F. Let tC denote a left-handed Dehn twist about C. A fractional power of tC of exponent ℓ//n is an h ∈ Mod(Sg) such that hn = tCℓ. Unlike a root of a tC, a fractional power h can exchange the sides of C. We derive necessary and sufficient conditions for the existence of both side-exchanging and side-preserving fractional powers. We show in the side-preserving case that if gcd(ℓ,n) = 1, then h will be isotopic to the ℓth power of an nth root of tC and that n ≤ 2g+1. In general, we show that n ≤ 4g, and that side-preserving fractional powers of exponents 2g//2g+2 and 2g//4g always exist. For a side-exchanging fractional power of exponent ℓ//2n, we show that 2n ≥ 2g+2, and that side-exchanging fractional powers of exponent 2g+2//4g+2 and 4g+1//4g+2 always exist. We give a complete listing of certain side-preserving and side-exchanging fractional powers on S5.
Haemonchus contortusas a paradigm and model to study anthelmintic drug resistance
Authors: GILLEARD JOHN S. 
Published: September 2013

SUMMARYAnthelmintic resistance is a major problem for the control livestock parasites and a potential threat to the sustainability of community-wide treatment programmes being used to control human parasites in the developing world. Anthelmintic resistance is essentially a complex quantitative trait in which multiple mutations contribute to the resistance phenotype in an additive manner. Consequently, a combination of forward genetic and genomic approaches are needed to identify the causal mutations and quantify their contribution to the resistance phenotype. Therefore, there is a need to develop genetic and genomic approaches for key parasite species identified as relevant models.Haemonchus contortus, a gastro-intestinal parasite of sheep, has shown a remarkable propensity to develop resistance to all the drugs used in its control. Partly because of this, and partly because of its experimental amenability, research on this parasite has contributed more than any other to our understanding of anthelmintic resistance.H. contortusoffers a variety of advantages as an experimental system including the ability to undertake genetic crosses; a prerequisite for genetic mapping. This review will discuss the current progress on developingH. contortusas a model system in which to study anthelmintic resistance.
Improvement of speeded-up robust features for robot visual simultaneous localization and mapping
Authors: Wang Yin-Tien , Lin Guan-Yu 
Published: September 2013

SUMMARYA robot mapping procedure using a modified speeded-up robust feature (SURF) is proposed for building persistent maps with visual landmarks in robot simultaneous localization and mapping (SLAM). SURFs are scale-invariant features that automatically recover the scale and orientation of image features in different scenes. However, the SURF method is not originally designed for applications in dynamic environments. The repeatability of the detected SURFs will be reduced owing to the dynamic effect. This study investigated and modified SURF algorithms to improve robustness in representing visual landmarks in robot SLAM systems. Many modifications of the SURF algorithms are proposed in this study including the orientation representation of features, the vector dimension of feature description, and the number of detected features in an image. The concept of sparse representation is also used to describe the environmental map and to reduce the computational complexity when using extended Kalman filter (EKF) for state estimation. Effective procedures of data association and map management for SURFs in SLAM are also designed to improve accuracy in robot state estimation. Experimental works were performed on an actual system with binocular vision sensors to validate the feasibility and effectiveness of the proposed algorithms. The experimental examples include the evaluation of state estimation using EKF SLAM and the implementation of indoor SLAM. In the experiments, the performance of the modified SURF algorithms was compared with the original SURF algorithms. The experimental results confirm that the modified SURF provides better repeatability and better robustness for representing the landmarks in visual SLAM systems.
Coxeter groups and Kähler groups
Authors: PY PIERRE 
Published: September 2013

AbstractWe study homomorphisms from Kähler groups to Coxeter groups. As an application, we prove that a cocompact complex hyperbolic lattice (in complex dimension at least 2) does not embed into a Coxeter group or a right-angled Artin group. This is in contrast with the case ofrealhyperbolic lattices.
Immunization against recombinant GnRH-I alters testicular structure in an experimental boar model
Authors: Li Yunsheng , Liu Ya , Su Shiping , Pu Yong , Zhang Xiaorong , Fang Fugui 
Published: September 2013

SummaryThe aim of this study was to evaluate and to compare testicular tissue in immunized and control boars. Eighteen male piglets, aged 12 weeks, were vaccinated twice intramuscularly with a maltose-binding protein–gonadotropin-releasing hormone I hexamer peptide (MBP-GnRH-I6). Blood samples were taken at 12, 18, 21 and 24 weeks of age. Serum concentrations of testosterone and GnRH-I antibodies were determined by radioimmunoassay. The pigs were sacrificed 6 weeks after the second immunization. Testicular weight and size were recorded and tissue samples were collected for histological examination. The results demonstrated that active immunization against MBP-GnRH-I6 increased serum GnRH-I antibody levels (P < 0.05) and reduced serum concentrations of testosterone (P < 0.05) when compared with controls. Histological studies performed on testicular tissue revealed clear signs of atrophy in the MBP-GnRH-I6 immunized pigs, and a significant reduction (P < 0.05) in paired testes weight and size were seen in the treated boars. Microscopically, the mean diameter of the seminiferous tubules was markedly reduced (P < 0.01). Spermatogonia were visible, as well as few spermatocytes, but no spermatozoa were detected in the seminiferous tubules. Ultramicroscopic analysis of testicular tissue revealed an increase in the thickness of the basement membrane and extensive damage in the cell organelles of the treated animals, including small spermatogonial size, decreased number of mitochondria and endoplasmic reticulum in the primary spermatocyte and spermatid, a shallow hollow for nuclear membranes in Sertoli cells and mitochondrial vacuolation in Leydig cells. We conclude that MBP-GnRH-I6 induces severe atrophy in the testes of immunized boars.
Embryological, clinical and ultrastructural study of human oocytes presenting indented zona pellucida
Authors: Sousa M. , da Silva J. Teixeira , Silva J. , Cunha M. , Viana P. , Oliveira E. , Sá R. , Soares C. , Oliveira C. , Barros A. 
Published: September 2013

SummaryHuman oocyte dysmorphisms attain a large proportion of retrieved oocytes from assisted reproductive technology (ART) treatment cycles. Extracytoplasmic defects involve abnormal morphology of the zona pellucida (ZP), perivitelline space and first polar body. The aim of the present study was to describe a novel dysmorphism affecting the ZP, indented ZP. We also evaluated the clinical, embryological and ultrastructural features of these cases. We evaluated all ART treatment cycles during 7 consecutive years and found 13 treatment cycles (six patients) with all oocytes presenting an indented ZP. In addition, these oocytes presented total or partial absence of the perivitelline space, absence of resistance to ZP and oolemma penetration during microinjection, and low ooplasm viscosity during aspiration. This novel described dysmorphism was recurrent and attained all oocytes in three cases that had more than one treatment cycle. When compared with controls, data showed significant low oocyte maturity (42% versus 81.6%) and high cycle cancellation (30.8% versus 8.5%) rates, normal degeneration (3.4% versus 6.3%) and fertilization rates (69% versus 69.5%), and low pregnancy (15.4% versus 33.3%) and live-birth delivery (7.7% versus 27.7%) rates per cycle. Ultrastructure analysis revealed a zona pellucida structure with large empty electrolucent regions, an outer ZP layer with an indented surface with protuberances and a thick inner ZP that obliterated the perivitelline space. There was evidence of exocytosis of ZP material by the oocyte. In conclusion, oocytes with this novel described dysmorphism (indented ZP) are associated with low maturity, pregnancy and live-birth delivery rates.
Bacteria Mediate Oviposition by the Black Soldier Fly, Hermetia illucens (L.), (Diptera: Stratiomyidae)
Authors: Zheng Longyu , Crippen Tawni L. , Holmes Leslie , Singh Baneshwar , Pimsler Meaghan L. , Benbow M. Eric , Tarone Aaron M. , Dowd Scot , Yu Ziniu , Vanlaerhoven Sherah L. , Wood Thomas K. , Tomberlin Jeffery K. 
Published: September 2013

AbstractThere can be substantial negative consequences for insects colonizing a resource in the presence of competitors. We hypothesized that bacteria, associated with an oviposition resource and the insect eggs deposited on that resource, serve as a mechanism regulating subsequent insect attraction, colonization and potentially succession of insect species. We isolated and identified bacterial species associated with insects associated with vertebrate carrion and used these bacteria to measure their influence on the oviposition preference of adult black soldier flies which utilizes animal carcasses and is an important species in waste management and forensics. We also ascertained that utilizing a mixture of bacteria, rather than a single species, differentially influenced behavioral responses of the flies, as did bacterial concentration and the species of fly from which the bacteria originated. These studies provide insight into interkingdom interactions commonly occurring during decomposition, but not commonly studied.
Impacts of Diet on Thiamine Status of Lake Ontario American Eels
Authors: Fitzsimons John D. , Brown Scott B. , Brown Lisa R. , Verreault Guy , Tardif Rémi , Drouillard Ken G. , Rush Scott A. , Lantry Jana R. 
Published: September 2013

AbstractThe Lake Ontario–upper St. Lawrence River (LOUSL) population of American Eels Anguilla rostrata (hereafter, “eels”) was once one of the most important groups of the species but is now in a state of serious decline. Given that thiamine deficiency has been observed in almost all of the top predators in Lake Ontario, we assessed the potential that a diet‐induced thiamine deficiency associated with consumption of Alewives Alosa pseudoharengus could be involved. Muscle thiamine was measured in eels from throughout the LOUSL corridor and was compared with putative threshold effect levels established from the literature. Mirex concentrations were used to separate Lake Ontario‐resident eels from non‐Lake Ontario‐resident eels. Stable isotope analyses of eel muscle samples and potential prey, including Alewives, were combined with mixing model software to infer the diets consumed by Lake Ontario eels. Although residence in Lake Ontario was associated with a significant decline in muscle thiamine concentration, estimated Alewife consumption by eels was unexpectedly low. Instead, mixing model results indicated that crayfish and Round Goby Neogobius melanostomus were the major prey. Both taxa are known to contain thiaminase and have the potential to cause thiamine deficiency, but there are no reports of thiamine deficiency associated with Round Goby consumption, thus implicating crayfish. There was no recovery in thiamine levels prior to the initiation of oceanic migration. As a result, thiamine levels of Lake Ontario‐resident eels prior to migration were only slightly above putative threshold effect levels for pathological and behavioral effects in Japanese Eels Anguilla japonica, but this would require confirmation with American Eels. Since thiamine levels are expected to decline further during migration, additional effects on eel behavior, reproduction, and survival seem probable. Because of panmixia, such effects—when combined with the relatively high reproductive potential of Lake Ontario‐resident eels—may have consequences for the entire species.
Experimental Transmission of VHSV Genotype IVb by Predation
Authors: Getchell Rodman G. , Cornwell Emily R. , Groocock Geoffrey H. , Wong Po Ting , Coffee Laura L. , Wooster Gregory A. , Bowser Paul R. 
Published: September 2013

Abstract
Preliminary surveillance of wild baitfish during the 2006 viral hemorrhagic septicemia virus genotype IVb (VHSV IVb) outbreaks indicated Emerald Shiners Notropis atherinoides and Bluntnose Minnow Pimephales notatus were infected with high levels of VHSV without showing clinical signs of disease. The movement and use of baitfish was recognized as the most probable vector for the introduction of VHSV to inland waters, such as Conesus Lake and Skaneateles Lake in New York, Budd Lake in Michigan, and Little Lake Butte des Morts and Lake Winnebago in Wisconsin. While numerous government agencies implemented restrictions to stop the movement of potentially infected baitfish into new waters and prevent the spread of VHSV IVb, until now, studies to investigate whether these initial introductions were by an oral route of infection have not occurred. Our studies identified infected Fathead Minnow Pimephales promelas as suitable vectors for transmitting VHSV IVb when fed to Tiger Muskellunge (♂ Northern Pike Esox lucius × ♀ Muskellunge Esox masquinongy) during laboratory trials. Six of 16 Tiger Muskellunge were infected with VHSV IVb after consumption of infected Fathead Minnows when assayed with quantitative reverse transcriptase polymerase chain reaction and viral isolation in cell culture. Weekly sampling of water and feces from these Tiger Muskellunge individually reared showed intermittent shedding of VHSV IVb. Those exposed to similarly VHSV IVb-inoculated fathead minnows by cohabitation only became infected in 1 case out of 16. A similar trial of 12 Tiger Muskellunge fed Round Goby Neogobius melanostomus that survived a VHSV IVb immersion challenge did not result in infection. Overall, our findings imply that consumption of infected wild baitfish may be a risk factor for introduction of VHSV.
Received April 3, 2013; accepted May 27, 2013
Impacts of the Lacey Act Amendment and the Voluntary Partnership Agreements on illegal logging: implications for global forest governance
Authors: Gan Jianbang , Cashore Benjamin , Stone Michael W. 
Published: September 2013

Abstract
Illegal logging has negatively impacted environmental, economic, and social conditions in forests around the world. Various policies have been stymied owing to the complex web of timber harvesting, processing, manufacturing, and consumption cutting across developed and developing countries. We examine the potential impacts of new initiatives that directly address illegal timber trade by the two largest importers, primarily the ‘Lacy Act Amendment’ (LAA) by the US and the ‘Voluntary Partnership Agreements’ (VPAs) by the EU. We assess their effects on economy-wide welfare, prices of wood and paper products, and timber harvests in individual countries/regions and the world. We estimate that the LAA and VPAs, by themselves, would have moderate impacts on reducing illegal logging (0.1–8.35% reductions in timber harvests in the countries with high illegal logging rates). Broader cooperation among producer and consumer countries and alignment of existing forest governance mechanisms with illegal logging control measures are critical to create synergies.
Explosive reconnection of double tearing modes in relativistic plasmas: application to the Crab flares
Authors: Baty H. , Petri J. , Zenitani S. 
Published: August 2013

Abstract
Magnetic reconnection associated to the double tearing mode is investigated by means of resistive relativistic magnetohydrodynamic simulations. A linearly unstable double current sheet system in two-dimensional Cartesian geometry is considered. For initial perturbations of large enough longitudinal wavelengths, a fast reconnection event is triggered by a secondary instability that is structurally driven by the non-linear evolution of the magnetic islands. The latter reconnection phase and time-scale appear to weakly depend on the plasma resistivity and magnetization parameter. We discuss the possible role of such explosive reconnection dynamics to explain the MeV flares observed in the Crab Pulsar nebula. Indeed, the time-scale and the critical minimum wavelength give constraints on the Lorentz factor of the striped wind and on the location of the emission region, respectively.
Identification of Myosin XI Receptors in Arabidopsis Defines a Distinct Class of Transport Vesicles
Authors: Peremyslov Valera V. , Morgun Eva A. , Kurth Elizabeth G. , Makarova Kira S. , Koonin Eugene V. , Dolja Valerian V. 
Published: August 2013

Abstract
To characterize the mechanism through which myosin XI-K attaches to its principal endomembrane cargo, a yeast two-hybrid library of Arabidopsis thaliana cDNAs was screened using the myosin cargo binding domain as bait. This screen identified two previously uncharacterized transmembrane proteins (hereinafter myosin binding proteins or MyoB1/2) that share a myosin binding, conserved domain of unknown function 593 (DUF593). Additional screens revealed that MyoB1/2 also bind myosin XI-1, whereas myosin XI-I interacts with the distantly related MyoB7. The in vivo interactions of MyoB1/2 with myosin XI-K were confirmed by immunoprecipitation and colocalization analyses. In epidermal cells, the yellow fluorescent protein–tagged MyoB1/2 localize to vesicles that traffic in a myosin XI–dependent manner. Similar to myosin XI-K, MyoB1/2 accumulate in the tip-growing domain of elongating root hairs. Gene knockout analysis demonstrated that functional cooperation between myosin XI-K and MyoB proteins is required for proper plant development. Unexpectedly, the MyoB1-containing vesicles did not correspond to brefeldin A–sensitive Golgi and post-Golgi or prevacuolar compartments and did not colocalize with known exocytic or endosomal compartments. Phylogenomic analysis suggests that DUF593 emerged in primitive land plants and founded a multigene family that is conserved in all flowering plants. Collectively, these findings indicate that MyoB are membrane-anchored myosin receptors that define a distinct, plant-specific transport vesicle compartment.
bZIP67 Regulates the Omega-3 Fatty Acid Content of Arabidopsis Seed Oil by Activating FATTY ACID DESATURASE3
Authors: Mendes Ana , Kelly Amélie A. , van Erp Harrie , Shaw Eve , Powers Stephen J. , Kurup Smita , Eastmond Peter J. 
Published: August 2013

Abstract
Arabidopsis thaliana seed maturation is accompanied by the deposition of storage oil, rich in the essential ω-3 polyunsaturated fatty acid α-linolenic acid (ALA). The synthesis of ALA is highly responsive to the level of FATTY ACID DESATURASE3 (FAD3) expression, which is strongly upregulated during embryogenesis. By screening mutants in LEAFY COTYLEDON1 (LEC1)–inducible transcription factors using fatty acid profiling, we identified two mutants (lec1-like and bzip67) with a seed lipid phenotype. Both mutants share a substantial reduction in seed ALA content. Using a combination of in vivo and in vitro assays, we show that bZIP67 binds G-boxes in the FAD3 promoter and enhances FAD3 expression but that activation is conditional on bZIP67 association with LEC1-LIKE (L1L) and NUCLEAR FACTOR-YC2 (NF-YC2). Although FUSCA3 and ABSCISIC ACID INSENSITIVE3 are required for L1L and bZIP67 expression, neither protein is necessary for [bZIP67:L1L:NF-YC2] to activate FAD3. We conclude that a transcriptional complex containing L1L, NF-YC2, and bZIP67 is induced by LEC1 during embryogenesis and specifies high levels of ALA production for storage oil by activating FAD3 expression.