bluuebunny/crossref_metadata_2025_split huggingface dataset:
lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer
Authors: Liu Yongchao , Zhao Jing , Zhang Wenhong , Gan Jun , Hu Chengen , Huang Guangjian , Zhang Ying 
Published: May 2015

AbstractLong non-coding RNAs (lncRNAs), which have evolved as important gene expression modulators, are involved in human malignancies. The down-regulation of lncRNA growth arrest specific transcript 5 (GAS5) has been reported in several cancers, however, the underlying mechanism of lncRNA GAS5 in stomach cancer is poorly understood. In this study, we found that lncRNA GAS5 had lower expression in stomach cancer tissues than the normal counterparts. lncRNA GAS5 was shown to interact with Y-box binding protein 1 (YBX1) and lncRNA GAS5 knockdown was shown to accelerate YBX1 protein turnover without affecting YBX1 transcription. lncRNA GAS5 down-regulation reduced the YBX1 protein level, which decreased YBX1-transactivated p21 expression and abolished G1 phase cell cycle arrest in stomach cancer. These results delineate a novel mechanism of lncRNA GAS5 in suppressing stomach carcinogenesis and the lncRNA GAS5/YBX1/p21 pathway we discovered may provide useful targets for developing lncRNA-based therapies for stomach cancer.
A peptide N-terminal protection strategy for comprehensive glycoproteome analysis using hydrazide chemistry based method
Authors: Huang Junfeng , Qin Hongqiang , Sun Zhen , Huang Guang , Mao Jiawei , Cheng Kai , Zhang Zhang , Wan Hao , Yao Yating , Dong Jing , Zhu Jun , Wang Fangjun , Ye Mingliang , Zou Hanfa 
Published: May 2015

AbstractEnrichment of glycopeptides by hydrazide chemistry (HC) is a popular method for glycoproteomics analysis. However, possible side reactions of peptide backbones during the glycan oxidation in this method have not been comprehensively studied. Here, we developed a proteomics approach to locate such side reactions and found several types of the side reactions that could seriously compromise the performance of glycoproteomics analysis. Particularly, the HC method failed to identify N-terminal Ser/Thr glycopeptides because the oxidation of vicinal amino alcohol on these peptides generates aldehyde groups and after they are covalently coupled to HC beads, these peptides cannot be released by PNGase F for identification. To overcome this drawback, we apply a peptide N-terminal protection strategy in which primary amine groups on peptides are chemically blocked via dimethyl labeling, thus the vicinal amino alcohols on peptide N-termini are eliminated. Our results showed that this strategy successfully prevented the oxidation of peptide N-termini and significantly improved the coverage of glycoproteome.
Tubulin structure-based drug design for the development of novel 4β-sulfur-substituted podophyllum tubulin inhibitors with anti-tumor activity
Authors: Zhao Wei , Bai Jia-Ke , Li Hong-Mei , Chen Tao , Tang Ya-Jie 
Published: May 2015

AbstractThe well-characterized anti-tubulin agent, podophyllotoxin (PTOX), with the 4′-position methoxyl group, targets the colchicines domain located between α- and β-tubulin. Two guanosine triphosphate (GTP) analogs of the tubulin-binding region were synthesized from PTOX, where a hydroxyl group was substituted with a carbon-sulfur bond. These compounds, 4-MP-PTOX and 4-TG-PTOX, reduce the dosage and greatly improve the therapeutic effect for microtubule damage in cancer cells. Here we characterize the anti-tubulin properties of these compounds. We found the stronger inhibition of tubulin polymerization (the concentration of 50% growth inhibition, GI50 < 2 μM) for compounds 4-TG-PTOX and 4-MP-PTOX, which were better than that of PTOX or colchicine. The cytotoxicity of two designed compounds on tumor cells was also significantly enhanced by comparing to those of PTOX and colchicines. The ΔH value of 4-MP-PTOX and 4-TG-PTOX binding to tubulin by isothermal titration calorimetry (ITC) was found to be −7.4 and −5.3 kcal·mol−1, respectively. The wide range of enthalpy values across the series may reflect entropy/enthalpy compensation effects. Fragments 6-mercaptopurine (MP) and 6-thioguanine (TG) likely enhance the affinity of 4-MP-PTOX and 4-TG-PTOX binding to tubulin by increasing the number of binding sites. The correctness of rational drug design was strictly demonstrated by a bioactivity test.
K63 linked ubiquitin chain formation is a signal for HIF1A degradation by Chaperone-Mediated Autophagy
Authors: Vasco Ferreira Joao , Rosa Soares Ana , Silva Ramalho Jose , Pereira Paulo , Girao Henrique 
Published: May 2015

AbstractChaperone-Mediated Autophagy is a selective form of autophagy. Recently, the degradation of a newly identified CMA substrate, the HIF1A transcription factor, was found to be regulated by the ubiquitin ligase STUB1. In this study we show, for the first time, that K63 ubiquitination is necessary for CMA degradation of HIF1A in vitro and in vivo. Additionally, STUB1 mediates K63 linked ubiquitination of HIF1A. Our findings add a new regulatory step and increase the specificity of the molecular mechanism involved in CMA degradation of HIF1A, expanding the role of ubiquitination to yet another biological process, since the same mechanism might be applicable to other CMA substrates.
Seasonal recharge and mean residence times of soil and epikarst water in a small karst catchment of southwest China
Authors: Hu Ke , Chen Hongsong , Nie Yunpeng , Wang Kelin 
Published: May 2015

AbstractSoil and epikarst play an important role in the hydrological cycle in karst regions. This paper focuses on investigating the seasonal recharge and mean residence time (MRT) of soil water and epikarst water in a small karst catchment of southwest China. The deuterium contents in precipitation, creek, soil baseflow (direct recharge of the saturated soil water to the stream), epikarst spring and soil waters were monitored weekly for two years and MRT was calculated by an exponential model (EM) and a dispersion model (DM). The obvious seasonal variation of deuterium in rainfall was buffered in epikarst water, indicating sufficient water mixing. Soil baseflow contained less rainy-season rainwater than epikarst spring discharge, reflecting the retarded effect of soil thickness on rainwater recharge. MRTs of all water bodies were 41-71 weeks and soils in the depression extended those of shallow groundwater. This demonstrated that the deep soil layer played an important role in karst hydrological processes in the study catchment. The creek was recharged mostly by rainfall through epikarst, indicating its crucial role in water circulation. These results showed epikarst had a strong water-holding capacity and also delayed water contact time with dolomite.
Ambipolar light-emitting organic single-crystal transistors with a grating resonator
Authors: Maruyama Kenichi , Sawabe Kosuke , Sakanoue Tomo , Li Jinpeng , Takahashi Wataru , Hotta Shu , Iwasa Yoshihiro , Takenobu Taishi 
Published: May 2015

AbstractElectrically driven organic lasers are among the best lasing devices due to their rich variety of emission colors as well as other advantages, including printability, flexibility and stretchability. However, electrically driven lasing in organic materials has not yet been demonstrated because of serious luminescent efficiency roll-off under high current density. Recently, we found that the organic ambipolar single-crystal transistor is an excellent candidate for lasing devices because it exhibits less efficient roll-off, high current density and high luminescent efficiency. Although a single-mode resonator combined with light-emitting transistors (LETs) is necessary for electrically driven lasing devices, the fragility of organic crystals has strictly limited the fabrication of resonators and LETs with optical cavities have never been fabricated until now. To achieve this goal, we improved the soft ultraviolet-nanoimprint lithography method and demonstrated electroluminescence from a single-crystal LET with a grating resonator, which is a crucial milestone for future organic lasers.
CART treatment improves memory and synaptic structure in APP/PS1 mice
Authors: Jin Jia-li , Liou Anthony K.F. , Shi Yejie , Yin Kai-lin , Chen Ling , Li Ling-ling , Zhu Xiao-lei , Qian Lai , Yang Rong , Chen Jun , Xu Yun 
Published: May 2015

AbstractMajor characteristics of Alzheimer’s disease (AD) include deposits of β-amyloid (Aβ) peptide in the brain, loss of synapses and cognitive dysfunction. Cocaine- and amphetamine-regulated transcript (CART) has recently been reported to attenuate Aβ-induced toxicity. In this study, CART localization in APP/PS1 mice was characterized and the protective effects of exogenous CART treatment were examined. Compared to age-matched wild type mice, 8-month-old APP/PS1 mice had significantly greater CART immunoreactivity in the hippocampus and cortex. A strikingly similar pattern of Aβ plaque-associated CART immunoreactivity was observed in the cortex of AD cases. Treatment of APP/PS1 mice with exogenous CART ameliorated memory deficits; this effect was associated with improvements in synaptic ultrastructure and long-term potentiation, but not a reduction of the Aβ plaques. Exogenous CART treatment in APP/PS1 mice prevented depolarization of the mitochondrial membrane and stimulated mitochondrial complex I and II activities, resulting in an increase in ATP levels. CART treatment of APP/PS1 mice also reduced reactive oxygen species and 4-hydroxynonenal and mitigated oxidative DNA damage. In summary, CART treatment reduced multiple neuropathological measures and improved memory in APP/PS1 mice and may therefore be a promising and novel therapy for AD.
Improved adaptive localisation approach for indoor positioning by using environmental thresholds with wireless sensor nodes
Authors: Koyuncu Hakan , Yang Shuang Hua 
Published: May 2015

Jennic type wireless sensor nodes (WSN) are utilised together with environmentally adaptive localisation algorithm (EAL), to determine the unknown target positions. Received signal strength indicator (RSSI) values are employed within their standard deviation boundaries around their mean. Static standard deviation threshold concept for RSSI values is introduced to adapt them to environmental ranging factors. Trigonometric techniques are utilised together with EAL algorithm to calculate the unknown target positions. Positional accuracies of around 1 m are obtained in this study.
The Relationship between Preoperative Expectations and the Short-Term Postoperative Satisfaction and Functional Outcome in Lumbar Spine Surgery: A Systematic Review
Authors: Ellis Daniel J. , Mallozzi Scott S. , Mathews Jacob E. , Moss Isaac L. , Ouellet Jean A. , Jarzem Peter , Weber Michael H. 
Published: May 2015

Study Design Systematic review. Objective To examine the relationship between the patient's preoperative expectations and short-term postoperative satisfaction and functional outcome in lumbar spine surgery. Methods The Medline, Embase, and Cochrane databases were queried using a predefined search algorithm to identify all lumbar spine studies analyzing the influence of preoperative expectations on postoperative satisfaction and functional outcome. Two independent reviewers and a third independent mediator reviewed the literature and performed study screening, selection, methodological assessment, and data extraction using an objective protocol. Results Of 444 studies identified, 13 met the inclusion criteria. Methodological quality scores ranged from 59 to 100% with the greatest variability in defining patient characteristics and the methods of assessing patient expectations. Patient expectations were assessed in 22 areas, most frequently back and leg pain expectations and general expectations. Functional outcome was assessed by 13 tools; the most common were the visual analog scale, Oswestry Disability Index (ODI), and Short Form Health Survey (SF-36). Positive expectations for symptomatology, activity, general health, and recovery correlated with satisfaction. General expectations correlated with higher SF-36 Physical Subcomponent scores, better global function, and lower ODI outcome. Conclusions on the influence of the expectations for pain were limited due to the study heterogeneity, but the evidence suggests a positive correlation between the expectation and outcome for back and leg pain. Conclusions Positive expectations correlated significantly with short-term postoperative satisfaction and functional outcome, including higher SF-36 scores, earlier return to work, and decreased ODI scores. Future expectation-based investigations will benefit from implementation of the standardized methods of expectation, satisfaction, and outcome analysis discussed herein.
Impact of a Multicomponent Screening, Brief Intervention, and Referral to Treatment (SBIRT) Training Curriculum on a Medical Residency Program
Authors: Kalu Nnenna , Cain Gloria , McLaurin-Jones TyWanda , Scott Denise , Kwagyan John , Fassassi Catsim , Greene Wendy , Taylor Robert E. 
Published: May 2015

Background Substance-related disorders are a growing problem in the United States. The patient-provider setting can serve as a crucial environment to detect and prevent at-risk substance use. Screening, brief intervention, and referral to treatment (SBIRT) is an integrated approach to deliver early intervention and treatment services for persons who have or are at risk for substance-related disorders. SBIRT training components can include online modules, in-person instruction, practical experience, and clinical skills assessment. This paper will evaluate the impact of multiple modes of training on acquisition of SBIRT skills as observed in a clinical skills assessment. Methods Residents were part of an SBIRT training program, from 2009 through 2013, consisting of lecture, role-play, online modules, patient encounters, and clinical skills assessment (CSA). Differences were assessed across satisfactory and unsatisfactory CSA performance. Results Seventy percent of the residents satisfactorily completed CSA. Demographics, type of components completed, and number of components completed were similar among residents who demonstrated satisfactory clinical skills compared with those who did not. All components of the training program were accepted equally across specialties and resident matriculation cohorts. Conclusion: The authors conclude that the components employed in SBIRT training do not have to be numerous or of a particular mode of training in order to see observable demonstration of SBIRT skills among medical residents. Thus, residency educators who have limited time or resources may utilize as few as 1 mode of training to effectually disseminate SBIRT skills among health care providers. As SBIRT continues to evolve as a promising tool to address at-risk substance-related disorders, it is critical to train medical residents and other health professionals.
Disentangling the magnetic force noise contribution in LISA Pathfinder
Authors: Armano M , Audley H , Auger G , Baird J , Binetruy P , Born M , Bortoluzzi D , Brandt N , Bursi A , Caleno M , Cavalleri A , Cesarini A , Cruise M , Danzmann K , Diepholz I , Dolesi R , Dunbar N , Ferraioli L , Ferroni V , Fitzsimons E , Freschi M , Gallegos J , García Marirrodriga C , Gerndt R , Gesa LI , Gibert F , Giardini D , Giusteri R , Grimani C , Harrison I , Heinzel G , Hewitson M , Hollington D , Hueller M , Huesler J , Inchauspé H , Jennrich O , Jetzer P , Johlander B , Karnesis N , Kaune B , Korsakova N , Killow C , Lloro I , Maarschalkerweerd R , Madden S , Mance D , Martín V , Martin-Porqueras F , Mateos I , McNamara P , Mendes J , Mendes L , Moroni A , Nofrarias M , Paczkowski S , Perreur-Lloyd M , Petiteau A , Pivato P , Plagnol E , Prat P , Ragnit U , Ramos-Castro J , Reiche J , Romera Perez J A , Robertson D , Rozemeijer H , Russano G , Sarra P , Schleicher A , Slutsky J , Sopuerta C F , Sumner T , Texier D , Thorpe J , Trenkel C , Tu H B , Vetrugno D , Vitale S , Wanner G , Ward H , Waschke S , Wass P , Wealthy D , Wen S , Weber W , Wittchen A , Zanoni C , Ziegler T , Zweifel P 
Published: May 2015

Abstract
Magnetically-induced forces on the inertial masses on-board LISA Pathfinder are expected to be one of the dominant contributions to the mission noise budget, accounting for up to 40%. The origin of this disturbance is the coupling of the residual magnetization and susceptibility of the test masses with the environmental magnetic field. In order to fully understand this important part of the noise model, a set of coils and magnetometers are integrated as a part of the diagnostics subsystem. During operations a sequence of magnetic excitations will be applied to precisely determine the coupling of the magnetic environment to the test mass displacement using the on-board magnetometers. Since no direct measurement of the magnetic field in the test mass position will be available, an extrapolation of the magnetic measurements to the test mass position will be carried out as a part of the data analysis activities. In this paper we show the first results on the magnetic experiments during an end-to-end LISA Pathfinder simulation, and we describe the methods under development to map the magnetic field on-board.

A post-publication change was made to this article on 26 Jun 2020 to add an author.
Bayesian statistics for the calibration of the LISA Pathfinder experiment
Authors: Armano M , Audley H , Auger G , Binetruy P , Born M , Bortoluzzi D , Brandt N , Bursi A , Caleno M , Cavalleri A , Cesarini A , Cruise M , Danzmann K , Diepholz I , Dolesi R , Dunbar N , Ferraioli L , Ferroni V , Fitzsimons E , Freschi M , García Marirrodriga C , Gerndt R , Gesa L , Gibert F , Giardini D , Giusteri R , Grimani C , Harrison I , Heinzel G , Hewitson M , Hollington D , Hueller M , Huesler J , Inchauspé H , Jennrich O , Jetzer P , Johlander B , Karnesis N , Kaune B , Korsakova N , Killow C , Lloro I , Maarschalkerweerd R , Madden S , Mance D , Martin V , Martin-Porqueras F , Mateos I , McNamara P , Mendes J , Mitchell E , Moroni A , Nofrarias M , Paczkowski S , Perreur-Lloyd M , Pivato P , Plagnol E , Prat P , Ragnit U , Ramos-Castro J , Reiche J , Romera Perez J A , Robertson D , Rozemeijer H , Russano G , Sarra P , Schleicher A , Slutsky J , Sopuerta C F , Sumner T , Texier D , Thorpe J , Trenkel C , Tu H B , Vetrugno D , Vitale S , Wanner G , Ward H , Waschke S , Wass P , Wealthy D , Wen S , Weber W , Wittchen A , Zanoni C , Ziegler T , Zweifel P 
Published: May 2015

Abstract
The main goal of LISA Pathfinder (LPF) mission is to estimate the acceleration noise models of the overall LISA Technology Package (LTP) experiment on-board. This will be of crucial importance for the future space-based Gravitational-Wave (GW) detectors, like eLISA. Here, we present the Bayesian analysis framework to process the planned system identification experiments designed for that purpose. In particular, we focus on the analysis strategies to predict the accuracy of the parameters that describe the system in all degrees of freedom. The data sets were generated during the latest operational simulations organised by the data analysis team and this work is part of the LTPDA Matlab toolbox.

A post-publication change was made to this article on 26 Jun 2020 to add an author.
Coregulation of tandem duplicate genes slows evolution of subfunctionalization in mammals
Authors: Lan Xun , Pritchard Jonathan K. 
Published: May 2015

AbstractGene duplication is a fundamental process in genome evolution. However, most young duplicates are degraded into pseudogenes by loss-of-function mutations, and the factors that allow some duplicate pairs to survive long-term remain controversial. One class of models to explain duplicate retention invokes sub- or neofunctionalization, especially through evolution of gene expression, while other models focus on sharing of gene dosage. While studies of whole genome duplications tend to support dosage sharing, the primary mechanisms in mammals–where duplications are small-scale and thus disrupt dosage balance– are unclear. Using RNA-seq data from 46 human and 26 mouse tissues we find that sub-functionalization of expression evolves slowly, and is rare among duplicates that arose within the placental mammals. A major impediment to subfunctionalization is that tandem duplicates tend to be co-regulated by shared genomic elements, in contrast to the standard assumption of modularity of gene expression. Instead, consistent with the dosage-sharing hypothesis, most young duplicates are down-regulated to match expression of outgroup singleton genes. Our data suggest that dosage sharing of expression is a key factor in the initial survival of mammalian duplicates, followed by slower functional adaptation enabling long-term preservation.
Value generation in B2B contexts: the SMEs’ perspective
Authors: Pérez Lourdes , Cambra-Fierro Jesús 
Published: May 2015

Purpose– The aim of this paper is to understand the process of value creation in business-to-business (B2B) contexts from the perspective of small- and medium-sized firms (SMEs). Small businesses are challenged to compete and collaborate with larger firms. While the “sharks” dilemma (often the most dangerous sharks also have the most valuable resources) focuses on specific defences, the authors emphasize a value generation perspective.Design/methodology/approach– The concept of asymmetric relationships is taken as a reference and examined using a longitudinal multi-case study.Findings– The authors results demonstrate how small firms not always assume an inferior, defensive position. Ambitious and growth-oriented SMEs learn to collaborate with larger partners and exhibit a proactive attitude towards relationship management. They understand the importance of developing social ties. They foster frequent and informal communication with their customers, favouring personal visits as a means to receive advice for directing their research efforts and exchange information and views. Such ties help them to develop shared plans and goals.Research limitations/implications– In asymmetric relationships, partner selection models should help firms to concentrate their efforts in a reduced group of key partners. These models should include not only economic performance indicators – variables such as flexibility and autonomy – but also innovation and improvement in processes, image, prestige and positioning, access to markets and stability.Originality/value– The authors found insight into a novel concept: dual-value appropriation, where partners do not split the pie of the total value generated, as frequently proposed in the literature, but fully appropriate a different and unique value from the relationship. The authors further highlight the important role played by the committed champions in developing communication and trust.