bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oferta e demanda de água na bacia hidrográfica do Rio Tibagi (BHRT) - Londrina, Paraná, Brasil: Saneamento Básico ou Produção de Energia
Authors: Zapparoli Irene Domenes 
Published: May 2013

O objetivo é analisar as demandas para o uso e reuso da água no município de Londrina e a produção de água e energia na Bacia Hidrográfica do Rio Tibagi (BHT), Paraná, Brasil. Os procedimentos metodológicos adotados são descritos a seguir: revisão bibliográfica, tendo como marco conceitual os princípios relativos à política pública ambiental e pesquisa documental; coleta de dados e análise dos resultados, tendo como fonte os documentos “Termo de Referência para Elaboração da Finalização do Plano da Bacia Hidrográfica do Rio Tibagi”, Projeto de Lei referente à criação no Município de Londrina do Projeto “Agricultor Protetor das Águas” e o “Programa de Conservação, Uso Racional e Reaproveitamento das Águas no Município de Londrina”. O estudo foca a Bacia Hidrográfica do Rio Tibagi, município de Londrina, Estado do Paraná, Brasil. Os resultados evidenciam que a conservação da bacia requer do estado e dos municípios leis e projetos de Gestão de Recursos Hídricos mais rigorosos, que os instrumentos existentes atualmente. O estudo permite concluir que a oferta de água enfrenta os interesses divergentes da produção de energia e do saneamento básico. O presente estudo não esgota o debate sobre o tema e sugere a continuidade das pesquisas de forma identificar e implementar instrumentos de compensação financeira para o atendimento do mercado de serviços ambientais que utiliza a água como matéria prima.
Adipose Tissue-Derived Multipotent Stromal Cells Have a Higher Immunomodulatory Capacity Than Their Bone Marrow-Derived Counterparts
Authors: Melief Sara M. , Zwaginga Jaap Jan , Fibbe Willem E. , Roelofs Helene 
Published: May 2013

Abstract
Adipose tissue-derived multipotent stromal cells (AT-MSCs) are studied as an alternative to bone marrow-derived multipotent stromal cells (BM-MSCs) for immunomodulatory treatment. In this study, we systematically compared the immunomodulatory capacities of BM-MSCs and AT-MSCs derived from age-matched donors. We found that BM-MSCs and AT-MSCs share a similar immunophenotype and capacity for in vitro multilineage differentiation. BM-MSCs and AT-MSCs showed comparable immunomodulatory effects as they were both able to suppress proliferation of stimulated peripheral blood mononuclear cells and to inhibit differentiation of monocyte-derived immature dendritic cells. However, at equal cell numbers, the AT-MSCs showed more potent immunomodulatory effects in both assays as compared with BM-MSCs. Moreover, AT-MSCs showed a higher level of secretion of cytokines that have been implicated in the immunomodulatory modes of action of multipotent stromal cells, such as interleukin-6 and transforming growth factor-β1. This is correlated with higher metabolic activity of AT-MSCs compared with BM-MSCs. We conclude that the immunomodulatory capacities of BM-MSCs and AT-MSCs are similar, but that differences in cytokine secretion cause AT-MSCs to have more potent immunomodulatory effects than BM-MSCs. Therefore, lower numbers of AT-MSCs evoke the same level of immunomodulation. These data indicate that AT-MSCs can be considered as a good alternative to BM-MSCs for immunomodulatory therapy.
Single‐Molecule Electrical Biosensors Based on Single‐Walled Carbon Nanotubes
Authors: Guo Xuefeng 
Published: May 2013

AbstractInteractions between biological molecules are fundamental to biology. Probing the complex behaviors of biological systems at the molecular level provides new opportunities to uncover the wealth of molecular information that is usually hidden in conventional ensemble experiments and address the “unanswerable” questions in the physical, chemical and biological sciences. Nanometer‐scale materials are particularly well matched with biomolecular interactions due to their biocompatibility, size comparability, and remarkable electrical properties, thus setting the basis for biological sensing with ultrahigh sensitivity. This brief review aims to highlight the recent progress of the burgeoning field of single‐molecule electrical biosensors based on nanomaterials, with a particular focus on single‐walled carbon nanotubes (SWNTs), for better understanding of the molecular structure, interacting dynamics, and molecular functions. The perspectives and key issues that will be critical to the success of next‐generation single‐molecule biosensors toward practical applications are also discussed, such as the device reproducibility, system integration, and theoretical simulation.
Lateral meningocele syndrome: Additional report and further evidence supporting a connective tissue basis
Authors: Alves Daniela , Sampaio Mafalda , Figueiredo Rita , Leão Miguel 
Published: May 2013

AbstractLateral meningocele syndrome is a rare disorder of unknown etiology, first described in 1977 and subsequently reported in nine other patients. These patients present distinctive craniofacial features and skeletal abnormalities in addition to multiple lateral meningoceles, suggesting a connective tissue disorder. Autosomal dominant inheritance is clearly suggested in one family and could explain familiar aggregation in another. We describe a simplex case of lateral meningocele syndrome with bicuspid aortic valve, supporting the hypothesis of a connective tissue basis for this disorder and further expanding the phenotype. © 2013 Wiley Periodicals, Inc.
Views and preferences for the implementation of non‐invasive prenatal diagnosis for single gene disorders from health professionals in the united kingdom
Authors: Hill Melissa , Karunaratna Madhavi , Lewis Celine , Forya Frida , Chitty Lyn 
Published: May 2013

AbstractNon‐invasive prenatal diagnosis (NIPD) will offer new options in prenatal diagnosis for carriers of single gene disorders. This will affect carrier couples and health professionals involved in prenatal care. The aim of this study was to determine health professional opinions on NIPD for single gene disorders to guide development of infrastructure required for implementation. A qualitative approach was adopted using focus groups (N = 17) and one‐to‐one interviews (N = 30) with health professionals from a range of backgrounds involved in caring for carriers of single gene disorders. Data were digitally recorded, transcribed verbatim and analyzed using thematic analysis. Participants were very positive about the introduction of NIPD, describing benefits arising from no risk of miscarriage, earlier testing and a simple procedure. A number of concerns for implementation were raised. Participants emphasized the need for the new test to be highly accurate and thoroughly validated. There was concern that people may not give as much thought to having a blood test compared to an invasive test or that it may be viewed as routine and as such NIPD may negatively impact on informed consent. In addition there was concern that the simplicity of a blood test may lead to increased pressure to test and terminate. However, participants felt these concerns could be overcome with thorough pre‐ and post‐test counseling. To ensure high quality care, offering NIPD through genetics or other specialist services is essential. Ongoing education and training of health professionals will be important, and guidelines and regulation are needed for effective implementation. © 2013 Wiley Periodicals, Inc.
First report of a de novo 18q11.2 microdeletion including GATA6 associated with complex congenital heart disease and renal abnormalities
Authors: Bui Peter H. , Dorrani Naghmeh , Wong Derek , Perens Gregory , Dipple Katrina M. , Quintero‐Rivera Fabiola 
Published: May 2013

AbstractDeletions of the long arm of chromosome 18 have been previously reported in many patients. Most cases involve the more distal regions of the long arm (18q21.1‐>qter). However, proximal interstitial deletions involving 18q11.2 are extremely rare. Here we report on a 14‐month‐old female with a 4.7 Mb (19,667,062–24,401,876 hg19) de novo interstitial deletion within chromosomal band 18q11.2, which includes GATA6 and 24 other RefSeq genes. The clinical features of our patient include complex congenital heart defects, a double outlet right ventricle, a subaortic ventricular septal defect, D‐malposed great arteries, an atrial septal defect, a dysplastic aortic valve and patent ductus arteriosus. In addition, she had renal anomalies—a duplicated collecting system on the left and mild right hydronephrosis. These heart and renal defects are not reported in other patients with 18q proximal interstitial deletions. Heterozygous point mutations in GATA6, encoding for a zinc finger transcription factor, have been shown to cause congenital heart defects. Given the well‐established biological role of GATA6 in cardiac development, a deletion of GATA6 is very likely responsible for our patient's complex congenital heart defects. This is the smallest and most proximal 18q11.2 deletion involving GATA6 that is associated with complex congenital heart disease and renal anomalies. © 2013 Wiley Periodicals, Inc.
Healthcare transition in patients with rare genetic disorders with and without developmental disability: Neurofibromatosis 1 and williams–beuren syndrome
Authors: Van Lierde Andrea , Menni Francesca , Bedeschi Maria Francesca , Natacci Federica , Guez Sophie , Vizziello Paola , Costantino Maria Antonella , Lalatta Faustina , Esposito Susanna 
Published: May 2013

AbstractThere are between 5,000 and 8,000 distinct rare diseases (RDs) affecting 6–8% of the population, most of which are caused by genetic defects. Many are highly complex, childhood‐onset, multi‐system disorders that are often associated with developmental disability, and require lifelong, highly specialized care and support. As larger numbers of children with previously fatal RDs survive into adulthood, they encounter significant challenges in transitioning from family‐centered, developmentally focused, multidisciplinary pediatric care to a less supportive adult healthcare system that is often unfamiliar with these conditions. This paper discusses the challenges of the transition from pediatric to adult health care in two groups of patients with multisystem genetic RDs (neurofibromatosis 1 [NF1] and Williams–Beuren syndrome [WBS]), and analyzes strategies for making the process easier for patients with and without developmental disabilities. Our findings show that there are still no guidelines in national healthcare programs on how to transition RD adolescents with and without developmental disabilities, and only a few pediatric centers have implemented the elements of transition in their general practice. Evidence regarding programs to facilitate transition is inconclusive and the transition from pediatric medicine to adult medicine for RDs remains a major challenge. However, transition requires both time and personnel, which are difficult to find in periods of fiscal austerity. Nevertheless, we should strongly advocate for governments investing more into transition infrastructure or they will face increased long‐term social and economic costs due to poor treatment compliance, disengagement from services, increased genetic risks, and higher rates of disease‐related complications. © 2013 Wiley Periodicals, Inc.
Multifractal analysis on dispersion of immiscible high‐density polyethylene/polystyrene blends processed via polymer vane plasticating extruder
Authors: Wu Zheng‐Huan , Zhao Yong‐Qing , Zhang Gui‐Zhen , Yang Zhi‐Tao , Qu Jin‐Ping 
Published: May 2013

ABSTRACTA 30 wt % high‐density polyethylene (HDPE)/70 wt % polystyrene (PS) blends and 5 wt % HDPE/95 wt % PS blends were prepared via polymer vane plasticating extruder, which can generate elongational force field, in different rotation speeds. The fracture surface of the HDPE/PS blends was observed by a scanning electron microscope, and the dispersion of the HDPE in the PS matrix was evaluated by multifractal programs. The multifractal spectrum width
, and the dimension difference of maximum and minimum probability subset
were defined to discuss the homogeneity and the diameter of the HDPE particles. The results showed that the multifractal method was an effective tool to quantitatively describe the special distribution and diameter of the dispersed particles. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2328–2335, 2013
Emodin Prevents Ethanol‐Induced Developmental Anomalies in Cultured Mouse Fetus through Multiple Activities
Authors: Yon Jung‐Min , Lin Chunmei , Oh Ki‐Wan , Baek Hong‐Seok , Lee Beom Jun , Yun Young Won , Nam Sang‐Yoon 
Published: May 2013

BACKGROUNDMaternal alcohol ingestion on pregnant period causes fetal alcohol syndrome including psychological and behavioral problems, and developmental abnormality. In this study, we investigated the effect of emodin, an active anthraquinone component found in the roots and bark of the genus Rhamnus (Buckthorn), on ethanol‐induced teratogenesis during embryonic organogenesis.METHODSWe cultured mouse embryos on embryonic day 8.5 for 2 days with ethanol (5 μl/3 ml) and/or emodin (1×10−5 and 1×10−4 μg/ml) using a whole embryo culture system and then investigated the developmental evaluation, superoxide dismutase (SOD) activity, and expression patterns of cytoplasmic SOD (SOD1), mitochondrial SOD (SOD2), cytosolic glutathione peroxidase (cGPx), tumor necrosis factor‐α (TNF‐α), caspase 3, and hypoxia inducible factor 1α (HIF‐1α).RESULTSMorphological parameters, including growth in yolk sac and fetal head, body length, and development of the central nervous system, circulation system, sensory organs, skeletal system, and limbs in embryos exposed to ethanol were significantly decreased compared to those of the normal control group, but co‐treatment with emodin (1 × 10−5 and 1 × 10−4 μg/ml) significantly improved these parameters. Furthermore, the reduced levels of SOD activity, and SOD1, SOD2, cGPx, and HIF‐1α and the increased gene levels of TNF‐α and caspase‐3 due to ethanol exposure were significantly restored by cotreatment with emodin. Birth Defects Res (Part B) 98:268–275, 2013. © 2013 Wiley Periodicals, Inc.CONCLUSIONSThis study revealed that cotreatment with emodin significantly prevented teratogenesis induced by ethanol, not only by modulating hypoxia and antioxidant enzymes, but also by attenuating the enhanced levels of TNF‐α and caspase 3 in cultured embryos. Therefore, emodin may be an effective preventive agent for ethanol‐induced teratogenesis. Birth Defects Res (Part B) 98:268–275, 2013. © 2013 Wiley Periodicals, Inc.
Clinical trial of combined radio- and fluorescence-guided sentinel lymph node biopsy in breast cancer
Authors: Schaafsma B E , Verbeek F P R , Rietbergen D D D , Hiel B , Vorst J R , Liefers G J , Frangioni J V , Velde C J H , Leeuwen F W B , Vahrmeijer A L 
Published: May 2013

Abstract

Background
Combining radioactive colloids and a near-infrared (NIR) fluorophore permits preoperative planning and intraoperative localization of deeply located sentinel lymph nodes (SLNs) with direct optical guidance by a single lymphatic tracer. The aim of this clinical trial was to evaluate and optimize a hybrid NIR fluorescence and radioactive tracer for SLN detection in patients with breast cancer.

Methods
Patients with breast cancer undergoing SLN biopsy were enrolled. The day before surgery, a periareolar injection of indocyanine green (ICG)–99mTc-radiolabelled nanocolloid was administered and a lymphoscintigram acquired. Blue dye was injected immediately before surgery. Intraoperative SLN localization was performed using a γ probe and the Mini-FLARETM NIR fluorescence imaging system. Patients were divided into two dose groups, with one group receiving twice the particle density of ICG and nanocolloid, but the same dose of radioactive 99mTc.

Results
Thirty-two patients were enrolled in the trial. At least one SLN was identified before and during operation. All 48 axillary SLNs could be detected by γ tracing and NIR fluorescence imaging, but only 42 of them stained blue. NIR fluorescence imaging permitted detection of lymphatic vessels draining to the SLN up to 29 h after injection. Doubling the particle density did not yield a difference in fluorescence intensity (median 255 (range 98–542) versus 284 (90–921) arbitrary units; P = 0.590) or signal-to-background ratio (median 5·4 (range 3·0–15·4) versus 4·9 (3·5–16·3); P = 1·000) of the SLN.

Conclusion
The hybrid NIR fluorescence and radioactive tracer permitted accurate preoperative and intraoperative detection of the SLNs in patients with breast cancer. Registration number: NTR3685 (Netherlands Trial Register;http://www.trialregister.nl).
Following the Evolution of Ru/Activated Carbon Catalysts during the Decomposition–Reduction of the Ru(NO)(NO3)3 Precursor
Authors: García‐García Francisco R. , Fernández‐García Marcos , Newton Mark A. , Rodríguez‐Ramos Inmaculada , Guerrero‐Ruiz Antonio 
Published: May 2013

AbstractOxygen surface groups, which are typically present on the surface of activated carbon, have a decisive effect on the achieved dispersion of active phases, which affects the electronic properties of the surface sites and stabilizes the materials against sintering phenomena. Therefore, the desorption of oxygen surface groups during the decomposition–reduction of the metal salt precursors of the fresh carbon‐based catalysts can lead to surface reconstruction of the metal nanoparticles, which can significantly affect their catalytic activity. In this work, the oxidation states of Ru atoms supported on both an activated carbon thermally treated to remove all oxygen groups and the untreated material were studied during the reduction process. The precursor to prepare these catalysts is Ru(NO)(NO3)3. The main results were obtained by using in situ X‐ray absorption near‐edge structure analysis of the Ru K‐edge under conditions comparable to the temperature‐programmed reduction experiments. The different reduction mechanism observed in each of the catalysts, of a multistep nature if oxygen surface groups are present and single‐step in the case of clean graphitic surfaces, can be regarded as a typical metal–support interaction. Moreover, differences in both the final Ru oxidation state and the subsequent interaction of the active phase with the support are the main cause of the five‐times higher catalytic activity observed in the presence of the catalyst without surface oxygen groups for the NH3 decomposition reaction.
Bioinspired Manganese and Iron Complexes with Tetradentate N Ligands for the Asymmetric Epoxidation of Olefins
Authors: Wang Xiaoe , Miao Chengxia , Wang Shoufeng , Xia Chungu , Sun Wei 
Published: May 2013

AbstractThe manganese and iron complexes with the tetradentate N ligand (1R,2R)‐N,N'‐dimethyl‐N,N'‐bis(1‐methyl‐2‐benzimidazolylmethyl)cyclohexane‐1,2‐diamine have been synthesized and characterized. The crystal structure of the manganese complex demonstrates a cis‐α configuration. Both the manganese and iron complexes are active catalysts for the asymmetric epoxidation of olefins with H2O2 as an oxidant and acetic acid as an additive. Up to 96 % ee was observed for the epoxidation of α,β‐unsaturated ketones at −20 °C.
Direct O2 Activation on Gold/Metal Oxide Catalysts through a Unique Double Linear OAuO Structure
Authors: Sun Keju , Kohyama Masanori , Tanaka Shingo , Takeda Seiji 
Published: May 2013

AbstractNanogold particles supported on metal oxide surfaces present unusually high catalytic performances in low‐temperature oxidation reactions. Despite numerous studies on that matter, the molecular mechanism concerning O2 activation remains controversial. Aimed to identify the active sites for direct O2 activation on gold, a AuOOAu structure was analyzed by Hückel theory. It was found that an increase of Au–O orbital interaction can significantly promote O2 adsorption and dissociation. Therefore, we constructed a realistic model with a unique double linear OsAuOa structure at the perimeter of the Au/metal oxide interfaces, which was examined subsequently by DFT calculations. The double linear OsAuOa structure exhibited high reactivity, with O2 dissociation barriers as low as 0.12 eV and 0.17 eV for Au/TiO2 and Au/CeO2 systems, respectively. The present work gives new insight into the reaction mechanism of low‐temperature oxidation reactions on gold catalysts.
Efficient One‐Pot Preparation of Cu‐SSZ‐13 Materials using Cooperative OSDAs for their Catalytic Application in the SCR of NOx
Authors: Martínez‐Franco Raquel , Moliner Manuel , Thogersen Joakim R. , Corma Avelino 
Published: May 2013

AbstractThe cooperative use of the Cu‐tetraethylenepentamine complex and N,N,N‐trimethyl‐1‐adamantammonium as organic structure‐directing agents (OSDAs) enabled the rationalized “one‐pot” preparation of Cu‐containing SSZ‐13 zeolites. A detailed study of different synthetic variables permitted the control of the Si/Al and Cu/(Si+Al) ratios in the final solids. Cu‐SSZ‐13 molecular sieves synthesized in alkaline media demonstrate excellent catalytic activities and good hydrothermal stabilities for the selective catalytic reduction of NOx. Finally, the effect of synthesis media on the catalytic active sites is also demonstrated and a remarkable activity loss for samples synthesized in fluoride media is observed.
Powerful Amino Acid Derived Bifunctional Phosphine Catalysts Bearing a Hydrogen Bond Donor in Asymmetric Synthesis
Authors: Xu Li‐Wen 
Published: May 2013

AbstractThis minireview focuses on the recent advances in the synthetic application of amino acid‐derived bifunctional phosphine catalysts bearing a hydrogen‐bond donor. In the examples illustrated, the amino acid‐based phosphines have already been proven to be versatile, catalyzing a wide range of asymmetric reactions, including (aza)‐MBH reactions, [3+2] cyclizations, [4+2] cycloadditions, allylic alkylation, and Michael addition.
New Nanostructured Materials: Synthesis of Dodecanuclear NiII Complexes and Surface Deposition Studies
Authors: Pons‐Balagué Alba , Piligkos Stergios , Teat Simon J. , Costa Jose Sánchez , Shiddiq Muhandis , Hill Stephen , Castro German R. , Ferrer‐Escorihuela Pilar , Sañudo E. Carolina 
Published: May 2013

AbstractMicrowave‐assisted synthesis has been used to obtain the family of dodecanuclear NiII complexes [Ni12(NO3)(MeO)12(MeC6H4CO2)9(MeOH)10(H2O)2][ClO4]2 (1), [Ni12(NO3)(MeO)12(BrC6H4CO2)9(MeOH)10(H2O)2][ClO4]2 (2), [Ni12(CO3)(MeO)12(MeC6H4CO2)9(MeOH)10(H2O)2]2[SO4] (3) and [Ni12(NO3)(MeO)12(MeC6H4CO2)9(MeOH)8(H2O)7][NO3]2 (4). They contain three {Ni4O4} cubane units which template around a central μ6 anion, either NO3− or CO32−. Their magnetic properties have been studied by superconducting quantum interference device (SQUID) magnetometry and high‐field EPR measurements. The nanostructuration of the Ni12 species on mica surfaces is studied by AFM and grazing‐incidence X‐ray diffraction, which reveal the formation of polycrystalline thin layers.
Forming Lipid Bilayer Membrane Arrays on Micropatterned Polyelectrolyte Film Surfaces
Authors: Zhang Ying , Wang Lei , Wang Xuejing , Qi Guodong , Han Xiaojun 
Published: May 2013

AbstractA novel method of forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces is introduced. Polyelectrolyte films were fabricated by the layer‐by‐layer technique on a silicon oxide surface modified with a 3‐aminopropyltriethoxysilane (APTES) monolayer. The surface pKa value of the APTES monolayer was determined by cyclic voltammetry to be approximately 5.61, on the basis of which a pH value of 2.0 was chosen for layer‐by‐layer assembly. Micropatterned polyelectrolyte films were obtained by deep‐UV (254 nm) photolysis though a mask. Absorbed fluorescent latex beads were used to visualize the patterned surfaces. Lipid bilayer arrays were fabricated on the micropatterned surfaces by immersing the patterned substrates into a solution containing egg phosphatidylcholine vesicles. Fluorescence recovery after photobleaching studies yielded a lateral diffusion coefficient for probe molecules of 1.31±0.17 μm2 s−1 in the bilayer region, and migration of the lipid NBD PE in bilayer lipid membrane arrays was observed in an electric field.