bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteopontin mediates macrophage chemotaxis via α4 and α9 integrins and survival via the α4 integrin
Authors: Lund Susan Amanda , Wilson Carole L. , Raines Elaine W. , Tang Jingjing , Giachelli Cecilia M. , Scatena Marta 
Published: November 2012

AbstractOsteopontin (OPN) is highly expressed by macrophages and plays a key role in the pathology of several chronic inflammatory diseases including atherosclerosis and the foreign body reaction. However, the molecular mechanism behind OPN regulation of macrophage functions is not well understood. OPN is a secreted molecule and interacts with several integrins via two domains: the RGD sequence binding to αv‐containing integrins, and the SLAYGLR sequence binding to α4β1, α4β7, and α9β1 integrins. Here we determined the role of OPN in macrophage survival, chemotaxis, and activation state. For survival studies, OPN treated‐bone marrow derived macrophages (BMDMs) were challenged with growth factor withdrawal and neutralizing integrin antibodies. We found that survival in BMDMs is mediated primarily through the α4 integrin. In chemotaxis studies, we observed that migration to OPN was blocked by neutralizing α4 and α9 integrin antibodies. Further, OPN did not affect macrophage activation as measured by IL‐12 production. Finally, the relative contributions of the RGD and the SLAYGLR functional domains of OPN to leukocyte recruitment were evaluated in an in vivo model. We generated chimeric mice expressing mutated forms of OPN in myeloid‐derived leukocytes, and found that the SLAYGLR functional domain of OPN, but not the RGD, mediates macrophage accumulation in response to thioglycollate‐elicited peritonitis. Collectively, these data indicate that α4 and α9 integrins interacting with OPN via the SLAYGLR domain play a key role in macrophage biology by regulating migration, survival, and accumulation. J. Cell. Biochem. 114: 1194–1202, 2013. © 2012 Wiley Periodicals, Inc.
Acquisition of paclitaxel resistance is associated with a more aggressive and invasive phenotype in prostate cancer
Authors: Kim John J. , Yin Bo , Christudass Christhunesa S. , Terada Naoki , Rajagopalan Krithika , Fabry Ben , Lee Danielle Y. , Shiraishi Takumi , Getzenberg Robert H. , Veltri Robert W. , An Steven S. , Mooney Steven M. 
Published: November 2012

AbstractDrug resistance is a major limitation to the successful treatment of advanced prostate cancer (PCa). Patients who have metastatic, castration‐resistant PCa (mCRPC) are treated with chemotherapeutics. However, these standard therapy modalities culminate in the development of resistance. We established paclitaxel resistance in a classic, androgen‐insensitive mCRPC cell line (DU145) and, using a suite of molecular and biophysical methods, characterized the structural and functional changes in vitro and in vivo that are associated with the development of drug resistance. After acquiring paclitaxel‐resistance, cells exhibited an abnormal nuclear morphology with extensive chromosomal content, an increase in stiffness, and faster cytoskeletal remodeling dynamics. Compared with the parental DU145, paclitaxel‐resistant (DU145‐TxR) cells became highly invasive and motile in vitro, exercised greater cell traction forces, and formed larger and rapidly growing tumors in mouse xenografts. Furthermore, DU145‐TxR cells showed a discrete loss of keratins but a distinct gain of ZEB1, Vimentin and Snail, suggesting an epithelial‐to‐mesenchymal transition. These findings demonstrate, for the first time, that paclitaxel resistance in PCa is associated with a trans‐differentiation of epithelial cell machinery that enables more aggressive and invasive phenotype and portend new strategies for developing novel biomarkers and effective treatment modalities for PCa patients. J. Cell. Biochem. 114: 1286–1293, 2013. © 2012 Wiley Periodicals, Inc.
The testicular sperm ducts and genital kidney of male Ambystoma maculatum (Amphibia, Urodela, Ambystomatidae)
Authors: Siegel Dustin S. , Aldridge Robert D. , Rheubert Justin L. , Gribbins Kevin M. , Sever David M. , Trauth Stanley E. 
Published: November 2012

AbstractThe ducts associated with sperm transport from the testicular lobules to the Wolffian ducts in Ambystoma maculatum were examined with transmission electron microscopy. Based on the ultrastructure and historical precedence, new terminology for this network of ducts is proposed that better represents primary hypotheses of homology. Furthermore, the terminology proposed better characterizes the distinct regions of the sperm transport ducts in salamanders based on anatomy and should, therefore, lead to more accurate comparisons in the future. While developing the above ontology, we also tested the hypothesis that nephrons from the genital kidney are modified from those of the pelvic kidney due to the fact that the former nephrons function in sperm transport. Our ultrastructural analysis of the genital kidney supports this hypothesis, as the basal plasma membrane of distinct functional regions of the nephron (proximal convoluted tubule, distal convoluted tubule, and collecting tubule) appear less folded (indicating decreased surface area and reduced reabsorption efficiency) and the proximal convoluted tubule possesses ciliated epithelial cells along its entire length. Furthermore, visible luminal filtrate is absent from the nephrons of the genital kidney throughout their entire length. Thus, it appears that the nephrons of the genital kidney have reduced reabsorptive capacity and ciliated cells of the proximal convoluted tubule may increase the movement of immature sperm through the sperm transport ducts or aid in the mixing of seminal fluids within the ducts. © J. Morphol., 2013. © 2012 Wiley Periodicals, Inc.
Impact of transvascular and cellular–interstitial water exchange on dynamic contrast‐enhanced magnetic resonance imaging estimates of blood to tissue transfer constant and blood plasma volume
Authors: Paudyal Ramesh , Poptani Harish , Cai Kejia , Zhou Rong , Glickson Jerry D. 
Published: November 2012

AbstractPurpose:To examine the effect of transvascular (kbe) and cellular–interstitial water protons exchange (kie) in estimates of the blood‐to‐tissue contrast agent transfer rate constant (Ktrans), interstitial volume fraction (ve), and blood plasma volume fraction (vp) using a full three‐site two exchange (3S2X) model.Materials and Methods:Using the Bloch‐McConnell equations, magnetic resonance imaging (MRI) signal arising from a 3S2X system was derived for the T1‐weighted spoiled gradient‐recalled echo (SPGR) pulse sequence. To model the effects of kbe and kie on estimates of R1, the MRI‐measured arterial input function, the different sets of values of kinetic parameters: Ktrans, ve, and vp and water protons exchange rates, kbe and kie, were used. To calculate the tissue water protons R1, the signal evolving from a 3S2X model was set to a monoexponential function. By comparing the estimated Ktrans, ve, and vp with their simulated model truth values, the impact of kbe and kie on Ktrans, ve, and vp was evaluated.Results:vp was strongly underestimated and Ktrans and ve were much less influenced by kbe, when kie was held constant. When kbe was held constant, the kie had a significant effect on Ktrans and ve and less effect on vp.Conclusion:The full 3S2X model allows accurate estimation of Ktrans, ve, and vp and rates of water proton exchange. J. Magn. Reson. Imaging 2013;37:435–444. © 2012 Wiley Periodicals, Inc.
Improved hepatic arterial phase MRI with 3‐second temporal resolution
Authors: Agrawal Mukta D. , Spincemaille Pascal , Mennitt Kevin W. , Xu Bo , Wang Yi , Dutruel Silvina P. , Prince Martin R. 
Published: November 2012

AbstractPurpose:To assess 3‐s temporal resolution for arterial phase bolus timing on dynamic liver MRI.Materials and Methods:One hundred consecutive patients undergoing fluoro‐triggered dynamic gadoxetate enhanced liver MRI with standard Cartesian k‐space LAVA (Liver Acquisition with Volume Acceleration) were compared with 61 consecutive patients imaged using spiral k‐space LAVA reconstructed at 3‐s temporal resolution with sliding window reconstruction. For qualitative analysis, bolus timing, hepatic artery branch order visualized, and overall image quality were evaluated. For quantitative analysis, contrast to noise ratio between aorta and liver parenchyma, aorta and portal vein, and signal intensity ratio between aorta and liver parenchyma were calculated.Results:MR fluoroscopy triggered single phase standard LAVA produced optimal arterial phase timing in 35% patients, compared with 88% with Spiral LAVA (P < 0.0001). Spiral LAVA had superior bolus timing scoring 2.0, compared with 1.0 with standard LAVA (P < 0.0001). Overall image quality and hepatic artery branch order visualization scoring were superior on spiral LAVA, compared with standard LAVA (P < 0.001). The aorta to liver parenchyma signal intensity ratio was also superior on spiral LAVA, compared with standard LAVA (2.8 vs. 2.2; P < 0.001).Conclusion:Dynamic liver MRI bolus timing improves using 3‐s temporal resolution. J. Magn. Reson. Imaging 2013;37:1129–1136. © 2013 Wiley Periodicals, Inc.
Predictors of clinical outcomes after surgical treatment of displaced acetabular fractures in the elderly
Authors: Zha Guo‐Chun , Sun Jun‐Ying , Dong Sheng‐Jie 
Published: November 2012

AbstractOutcomes following the open reduction and internal fixation (ORIF) of displaced acetabular fractures in the elderly have been inconsistent. Several factors associated with significant poor clinical outcomes have been reported; however, the factor that contributes independently to the outcomes remains unknown. This study aims to identify independent prognostic factors for the outcomes of surgically treated displaced acetabular fractures in the elderly. A total of 86 elderly patients with displaced acetabular fractures were treated by ORIF between May 1990 and June 2010. Matta's criteria and modified Merle d'Aubigne‐Postel score were used as reduction grades and clinical outcome measures, respectively. Reduction grades and six radiographic features were identified as prognostic factors. The six radiographic features include quadrilateral plate fracture, Gull sign, posterior dislocation of hip, femoral head injury (FHI), comminuted posterior wall fracture (CPWF), and posterior wall marginal impaction. The average clinical score of the patients associated with the six radiographic features was 14.4 ± 3.1, whereas the average clinical score for those without was 17.2 ± 1.6 points. The average clinical score of the patients with anatomical, imperfect, and poor reduction were 16.8 ± 2.4, 14.5 ± 2.3, and 11.3 ± 1.4 points, respectively. Multivariate analysis identified three independent predictors of clinical outcomes: reduction grades (t = −10.45, p = 0.000), CPWF (t = −2.74, p = 0.008), and FHI (t = −3.51, p = 0.000). Both CPWF and FHI are important risk factors for clinical outcome. The postoperative quality of reduction independently predicted patient prognosis, and anatomical reduction is predictive of a good prognosis. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 588–595, 2013
Gaseous emissions of nitrogen and carbon from urban vegetable gardens in Bobo‐Dioulasso, Burkina Faso
Authors: Lompo Désiré Jean‐Pascal , Sangaré Sheick Ahmed Khalil , Compaoré Emmanuel , Papoada Sedogo Michel , Predotova Martina , Schlecht Eva , Buerkert Andreas 
Published: November 2012

AbstractUrban and peri‐urban agriculture (UPA) is an important livelihood strategy for the urban poor in sub‐Saharan Africa and contributes to meeting increasing food demands in the rapidly growing cities. Although in recent years many research activities have been geared towards enhancing the productivity of this land‐use system, little is known about turnover processes and nutrient efficiency of UPA. The aim of our study therefore was to determine horizontal fluxes of N, P, K, and C as well as gaseous N and C emissions in urban vegetable gardens of Bobo‐Dioulasso, Burkina Faso. Two gardens referred to as “Kodéni” and “Kuinima” were selected as representative for urban and peri‐urban systems classified as: (1) “commercial gardening + field crops and livestock system” and (2) “commercial gardening and semicommercial field crop system”, respectively. A nutrient‐balance approach was used to monitor matter fluxes from March 2008 to March 2009 in both gardens. Ammonia (NH3), nitrous oxide (N2O) and carbon dioxide (CO2) emissions from the respective soils were measured during the coolest and the hottest period of the day using a closed‐chamber system. Annual partial balances amounted to 2056 kg N ha–1, 615 kg P ha–1, 1864 kg K ha–1, and 33 893 kg C ha–1 at Kodéni and to 1752 kg N ha–1, 446 kg P ha–1, 1643 kg K ha–1, and 21 021 kg C ha–1 at Kuinima. Emission rates were highest during the hot midday hours with peaks after fertilizer applications when fluxes of up to 1140 g NH3‐N ha–1 h–1, 154 g N2O‐N ha–1 h–1, 12 993 g CO2‐C ha–1 h–1 were recorded for Kodéni and Kuinima. Estimated annual gaseous N (NH3‐N + N2O‐N) and C (CO2‐C + CH4‐C) losses reached 419 kg N ha–1 and 35 862 kg C ha–1 at Kodéni and 347 kg N ha–1 and 22 364 kg C ha–1 at Kuinima. For both gardens, this represented 20% and 106% of the N and C surpluses, respectively. Emissions of NH3, largely emitted after surface application of manure and mineral fertilizers, accounted for 73% and 77% of total estimated N losses for Kodéni and Kuinima. To mitigate N losses nutrient‐management practices in UPA vegetable production of Bobo‐Dioulasso would greatly benefit from better synchronizing nutrient‐input rates with crop demands.
Genetic variability for responses to short‐ and long‐term salt stress in vegetative sunflower plants
Authors: Céccoli Gabriel , Eugenia Senn María , Bustos Dolores , Ismael Ortega Leandro , Córdoba Alicia , Vegetti Abelardo , Taleisnik Edith 
Published: November 2012

AbstractSunflower (Helianthus annuus L.) has been rated as moderately salt‐resistant, and variability for salt resistance has been detected within this crop. However, variability in salt‐resistance mechanisms has not been assessed. Osmotic tolerance, the relation of salt resistance with whole‐plant Na+ and K+ distribution and tissue Na+ tolerance were investigated in several sunflower inbred lines. Plants were grown under controlled conditions, in pots with sand and perlite irrigated with salinized (NaCl, –0.65 MPa) nutrient solution. Osmotic tolerance was assessed from the initial effects of the salt treatment on plant elongation in eleven sunflower lines. Long‐term salinity responses were evaluated in four of those lines, by assessing whole‐plant growth. A principal components analysis (PCA) was run on relative‐to‐control growth data, and this information was used to establish a relative resistance ranking, which indicated lines HAR2 > HAR1 > HA64 > HAR5. Osmotic tolerance was observed in HA64 and HAR2. The lines showed different degrees of Na+ accumulation, it was very low in some of them, but relative salt resistance was not associated to this trait. Tissue Na+ tolerance was deduced by comparing the percentage of dead leaves as a function of leaf blade Na+ accumulation, and it was higher in HAR1 than in the rest. These results indicate that variability for salt‐resistance mechanisms exists in sunflower. Osmotic tolerance and tissue Na+ tolerance were detected in different lines, highlighting that such variability may be exploited for increasing salt resistance in this crop.
Well‐differentiated thyroid carcinoma: The role of post‐operative radioactive iodine administration
Authors: Patel Supriya S , Goldfarb Melanie 
Published: November 2012

AbstractPost‐operative management of differentiated thyroid cancer (DTC) often involves administration of radioactive iodine (RAI) for remnant ablation or adjuvant therapy. However, given the favorable prognosis associated with DTC, the risk versus benefit ratio of RAI remains unclear. RAI is associated with substantial, albeit rare side effects, including a possible increased risk of secondary malignancy and altered fertility, which must be balanced against the magnitude of benefit for decreasing recurrence and improving survival. J. Surg. Oncol. 2013;107:665–672. © 2012 Wiley Periodicals, Inc.
Phenothiazine Semiconducting Polymer for Light‐Emitting Diodes
Authors: Elkassih Sussana A. , Sista Prakash , Magurudeniya Harsha D. , Papadimitratos Alexios , Zakhidov Anvar A. , Biewer Michael C. , Stefan Mihaela C. 
Published: November 2012

AbstractPhenothiazine alternating copolymer poly{4,8‐bis(5‐dodecylthiophene‐2‐yl)benzo[1,2‐b:4,5‐b′]dithiophene‐alt‐(10‐decylphenothiazine)} (P1) is synthesized by Stille coupling polymerization. The synthesized polymer has an electrochemical bandgap of 1.61 eV and displays good photoluminescence with a quantum yield of 63.3%. The polymer is tested as a light‐emitting material in polymeric light‐emitting diodes (PLEDs) with a device structure of indium tin oxide (ITO)/poly(3,4‐ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS)/polymer/Cs2CO3/Al. The devices display an orange‐yellow electroluminescence (Commission Internationale de l'Eclairage (CIE) 1931 color‐space chromaticity diagram: x = 0.5355 and y = 0.4611) with a maximum brightness of 3130 cd m−2 at an applied voltage of 10 V.
Investigation on Physical and Mechanical Properties of WPC from Corn stalk (Lignocellulosic Fiber) and HDPE
Authors: Khademieslam Habibollah , Khatibi Reza , Pour Arash Faraj 
Published: November 2012

AbstractSummary: This study has tried to use HDPE and the coupling agent consistent MAPP and cornstalk fibers, create wood plastic composite material and its physical and mechanical properties such as tensile modulus, flexural modulus and humidity absorption is measured. After determining the percentage of 20, 30 and 40% of corn stalk fibers in the product and the use of two longitudinal mesh levels of 40 and 80 of them and using the 5% MAPP coupling agent testing was done and it was shown that increasing fiber length and percent increase in product humidity is absorbed. This is while the declines by increasing the fiber length changes of samples were during the tensile tests. In the bending test also increased fiber length and flexural modulus was increased.
Validation of the Innsbruck REM sleep behavior disorder inventory
Authors: Frauscher Birgit , Ehrmann Laura , Zamarian Laura , Auer Florentine , Mitterling Thomas , Gabelia David , Brandauer Elisabeth , Delazer Margarete , Poewe Werner , Högl Birgit 
Published: November 2012

AbstractA diagnosis of definite REM sleep behavior disorder requires both a positive history for REM sleep behavior disorder and polysomnographic demonstration of REM sleep without atonia. To improve and facilitate screening for REM sleep behavior disorder, there is a need for simple clinical tools with sufficient sensitivity and specificity for the identification of subjects with probable REM sleep behavior disorder. We developed a short REM sleep behavior disorder screening questionnaire with 7 REM sleep behavior disorder– and 2 non‐REM sleep behavior disorder–specific control items and performed a validation study in 70 REM sleep behavior disorder subjects and 140 sleep disorder controls. Response patterns to all 7 REM sleep behavior disorder–specific items differed between REM sleep behavior disorder and non‐REM sleep behavior disorder patients (all P < 0.05), whereas the 2 non‐REM sleep behavior disorder–specific control items did not differentiate between REM sleep behavior disorder and non‐REM sleep behavior disorder (all P > .05). In 5 of the 7 REM sleep behavior disorder–specific items, AUC was greater than 0.700. These 5 items were included in the Innsbruck REM sleep behavior disorder inventory. In this questionnaire, a cutoff of 0.25 (number of positive symptoms divided by number of answered questions) had a sensitivity of 0.914 and a specificity of 0.857 for both idiopathic and Parkinson's‐related REM sleep behavior disorder (AUC, 0.886). The Innsbruck REM sleep behavior disorder inventory is a promising, easy‐to‐use, short screening tool for REM sleep behavior disorder with excellent sensitivity and specificity for both idiopathic and Parkinson's‐related REM sleep behavior disorder. © 2012 Movement Disorder Society
An evaluation of [F‐18]‐fluorodeoxy‐D‐glucose positron emission tomography, bone scan, and bone marrow aspiration/biopsy as staging investigations in Ewing Sarcoma
Authors: Newman Erik N. , Jones Robin L. , Hawkins Douglas S. 
Published: November 2012

AbstractBackgroundStaging investigations following the diagnosis of Ewing sarcoma may include chest computerized tomography (CT), technetium bone scintigraphy (bone scan), [F‐18]‐fluorodeoxy‐D‐glucose positron emission tomography (FDG‐PET) scan, and bone marrow biopsy and aspiration (BMA/Bx). Each of these staging investigations provides complementary prognostic information, however the optimal combination of staging investigations is not clear.ProcedureWe conducted a retrospective study of 91 patients diagnosed with Ewing sarcoma and consecutively treated at our medical facilities between January 1, 2001 and December 31, 2011. We compared the radiologist's interpretations of staging FDG‐PET and bone scans. We additionally compared the results of imaging evaluations to bilateral and unilateral BMA/Bx.ResultsWe found FDG‐PET and bone scan to have an examination‐based concordance rate of 98% (one discordant case with a positive FDG‐PET and negative bone scan). The region‐based concordance rate for the imaging modalities was 97% for all cases and 63% for metastatic cases. The ipsilateral concordance rate for BMA/Bx was 98% with BMBx detecting metastases in seven cases and BMA detecting metastases in four cases. The left versus right concordance rates for BMBx and BMA were 98% and 97%, respectively. In all cases where bone marrow metastases were detected by BMA or BMBx, FDG‐PET and bone scan detected osseous metastases.ConclusionsOur study indicates FDG‐PET may be sufficient for initial screening for osseous metastases and identified all patients who also have bone marrow metastases. If osseous metastases are detected, a bone scan can detect additional osseous lesions and BMBx may indicate prognostic bone marrow metastases. Pediatr Blood Cancer 2013; 60: 1113–1117. © 2012 Wiley Periodicals, Inc.
Chorioamniotic membrane separation after fetoscopic laser surgery for twin–twin transfusion syndrome
Authors: Egawa Makiko , Hayashi Satoshi , Yang Limin , Sakamoto Naoko , Sago Haruhiko 
Published: November 2012

ABSTRACTObjectiveThe purpose of our study was to investigate the incidence of chorioamniotic membrane separation (CMS) after fetoscopic laser surgery and the association between CMS and preterm premature rupture of membranes (pPROM). We also analyzed the risk factors associated with the occurrence of CMS.MethodRetrospective cohort study of 148 patients with twin–twin transfusion syndrome who underwent laser surgery at our institution from March 2003 to December 2009.ResultsChorioamniotic membrane separation occurred in 29 of 148 (19.6%) patients. The presence of CMS strongly correlated with pPROM prior to 28 weeks’ gestation. Multivariate analysis of the risk factors of pPROM <28 w showed for CMS an odds ratio (OR) of 7.01 (95% confidence interval (CI): [1.46; 33.60], p = 0.015). Posterior placentation correlated with the occurrence of CMS (OR: 4.17, 95% CI: [1.39; 12.49], p = 0.01) and the recipient's deepest vertical pocket (OR: 1.38, 95% CI: [1.03; 1.86], p = 0.03). There was however no measurable impact of CMS on gestational age at delivery, neither on survival.ConclusionChorioamniotic membrane separation occurs in approximately 20% of patients following fetoscopic laser surgery for twin–twin transfusion syndrome and is associated with pPROM <28 w. Posterior location of the placenta coincided with an elevated risk for CMS. © 2012 John Wiley & Sons, Ltd.