bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor sequestration in response to β-arrestin-2 phosphorylation by ERK1/2 governs steady-state levels of GPCR cell-surface expression
Authors: Paradis Justine S. , Ly Stevenson , Blondel-Tepaz Élodie , Galan Jacob A. , Beautrait Alexandre , Scott Mark G. H. , Enslen Hervé , Marullo Stefano , Roux Philippe P. , Bouvier Michel 
Published: September 2015

Significance
ERK1/2 are important G protein-coupled receptor (GPCR) signaling effectors, but their role as possible GPCR regulators remains largely uncharted. We report that ERK1/2 activation leads to the phosphorylation of β-arrestin-2 on Ser14 and Thr276, promoting the intracellular sequestration of unliganded GPCRs. This subcellular redistribution results in the dampening of cell responsiveness to GPCRs’ ligand-mediated activation, positioning ERK1/2 as both a downstream effector and a negative regulator of GPCRs. Because ERK1/2 also is stimulated by receptor tyrosine kinases and is deregulated in many diseases, and because GPCRs respond to a large number of hormones and neurotransmitters, this newly uncovered regulatory process is poised to play a central role in controlling cell responsiveness in health and disease.
Micromechanics of cellularized biopolymer networks
Authors: Jones Christopher A. R. , Cibula Matthew , Feng Jingchen , Krnacik Emma A. , McIntyre David H. , Levine Herbert , Sun Bo 
Published: September 2015

Significance
Mechanical interactions between cells, mediated by the elastic response of the extracellular matrix to active applied forces, play a critical role in developmental biology, wound healing, and cancer progression. This work applies sophisticated technical means, both in experiment and computational modeling, to investigate the micron-scale mechanics of a popular model of this medium, a collagen gel. The results obtained show clearly that on the cellular scale, there are significant spatial variations in the micromechanics due to network heterogeneities.
Electrostatic coating enhances bioavailability of insecticides and breaks pyrethroid resistance in mosquitoes
Authors: Andriessen Rob , Snetselaar Janneke , Suer Remco A. , Osinga Anne J. , Deschietere Johan , Lyimo Issa N. , Mnyone Ladslaus L. , Brooke Basil D. , Ranson Hilary , Knols Bart G. J. , Farenhorst Marit 
Published: September 2015

Significance
Conventional mosquito-control methods are becoming less effective in controlling mosquito-borne diseases because of widespread resistance against safe and recommended public health insecticides. Therefore innovative and effective alternatives are urgently needed. We present a novel method for exposing mosquitoes to insecticides that uses electrostatic forces to bind insecticide particles. Results show that this method increases exposure of mosquitoes to such an extent that even those that have developed high levels of resistance can be killed effectively. The ability to boost the efficacy of WHO-recommended insecticides provides a significant benefit to the field of vector-borne disease control and will further the development of novel resistance-breaking control tools.
Insect’s intestinal organ for symbiont sorting
Authors: Ohbayashi Tsubasa , Takeshita Kazutaka , Kitagawa Wataru , Nikoh Naruo , Koga Ryuichi , Meng Xian-Ying , Tago Kanako , Hori Tomoyuki , Hayatsu Masahito , Asano Kozo , Kamagata Yoichi , Lee Bok Luel , Fukatsu Takema , Kikuchi Yoshitomo 
Published: September 2015

Significance
In general, animals have a mouth for feeding, an anus for defecation, and a gut connecting them for digestion and absorption. However, we discovered that the stinkbug’s gut is functionally disconnected in the middle by a previously unrecognized organ for symbiont sorting, which blocks food fluid and nonsymbiotic bacteria but selectively allows passing of a specific bacterial symbiont. Though very tiny and inconspicuous, the organ governs the configuration and specificity of stinkbug gut symbiosis, wherein the posterior gut region is devoid of food flow, populated by a specific bacterial symbiont, and transformed into an isolated organ for symbiosis. Mutant analyses showed that the symbiont’s flagellar motility is needed for passing the host organ, highlighting intricate host–symbiont interactions underpinning the symbiont sorting process.
Label-free DNA imaging in vivo with stimulated Raman scattering microscopy
Authors: Lu Fa-Ke , Basu Srinjan , Igras Vivien , Hoang Mai P. , Ji Minbiao , Fu Dan , Holtom Gary R. , Neel Victor A. , Freudiger Christian W. , Fisher David E. , Xie X. Sunney 
Published: September 2015

SignificanceMicroscopic imaging of DNA has to rely on the use of fluorescent staining, an exogenous labeling in biological and biomedical studies, which often leads to uncertainty with respect to the quality and homogeneity of the staining. Label-free imaging of DNA will enable noninvasive visualization of live cell nuclei in both human and animals. Spontaneous Raman microspectroscopy offers label-free chemical contrast for DNA imaging; however, its slow imaging speed hampers its wide application for in vivo and dynamic studies. Here we developed a novel and simple approach with multicolor stimulated Raman scattering microscopy to evaluate rapid DNA imaging, which can be applied to both in vivo DNA dynamic studies and instant label-free human skin cancer diagnosis.
Mode of interaction of TRIP13 AAA-ATPase with the Mad2-binding protein p31comet and with mitotic checkpoint complexes
Authors: Miniowitz-Shemtov Shirly , Eytan Ety , Kaisari Sharon , Sitry-Shevah Danielle , Hershko Avram 
Published: September 2015

Significance
The mitotic checkpoint system is important to ensure accurate segregation of chromosomes in mitosis. It acts by the formation of a mitotic checkpoint complex (MCC), which inhibits the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). When the checkpoint is turned off, MCC is disassembled by the joint action of an ATP-utilizing enzyme, thyroid receptor interacting protein 13 (TRIP13), and a Mad2-binding protein, p31comet. It is not well understood how p31comet targets TRIP13 to disassemble MCC. We show here that p31comet and MCC mutually promote the binding of each other to oligomeric TRIP13 and propose a model for the mode of the action of TRIP13 in MCC disassembly. Thus, the results reveal an important molecular mechanism in the inactivation of the mitotic checkpoint.
Expression of collagenase in Flavobacterium psychrophilum isolated from cold-water disease-affected ayu (Plecoglossus altivelis)
Authors: Nakayama Hitoshi , Tanaka Keisuke , Teramura Naoko , Hattori Shunji 
Published: September 2015

Abstract
The collagenase activity and the fpcol gene were examined in Flavobacterium psychrophilum isolates from cold-water disease (CWD)-affected ayu, Plecoglossus altivelis. Collagenase expression was closely related to the accumulated mortality of CWD-affected ayu. RT-qPCR and bacterial challenge experiments showed that F. psychrophilum ayu isolate WA-1 expressed the fpcol gene more actively and was more virulent than ayu isolate WA-2. The amago (Oncorhynchus masou) isolate WB-1, which possesses a pseudo-fpcol gene, was not harmful to ayu. Hitherto, the well-studied metalloproteases Fpp1 and Fpp2 have been considered virulence factors. However, the most virulent isolate against ayu (WA-1) showed no Fpp activity because of a deletion mutation or an insertion of a transposon in the fpp genes. The less virulent WA-2 isolate showed only Fpp1 activity. Taken together, these results suggest that collagenolytic activity, but not Fpp activity, is related to the virulence of F. psychrophilum isolates in CWD-affected ayu.
A hybrid bayesian approach for genome-wide association studies on related individuals
Authors: Yazdani A. , Dunson D. B. 
Published: September 2015

AbstractMotivation: Both single marker and simultaneous analysis face challenges in GWAS due to the large number of markers genotyped for a small number of subjects. This large p small n problem is particularly challenging when the trait under investigation has low heritability.Method: In this article, we propose a two-stage approach that is a hybrid method of single and simultaneous analysis designed to improve genomic prediction of complex traits. In the first stage, we use a Bayesian independent screening method to select the most promising SNPs. In the second stage, we rely on a hierarchical model to analyze the joint impact of the selected markers. The model is designed to take into account familial dependence in the different subjects, while using local-global shrinkage priors on the marker effects.Results: We evaluate the performance in simulation studies, and consider an application to animal breeding data. The illustrative data analysis reveals an encouraging result in terms of prediction performance and computational cost.Contact: Akram.Yazdani@uth.tmc.eduSupplementary information: Supplementary data are available at Bioinformatics online.
NgsRelate: a software tool for estimating pairwise relatedness from next-generation sequencing data
Authors: Korneliussen Thorfinn Sand , Moltke Ida 
Published: September 2015

Abstract
Motivation: Pairwise relatedness estimation is important in many contexts such as disease mapping and population genetics. However, all existing estimation methods are based on called genotypes, which is not ideal for next-generation sequencing (NGS) data of low depth from which genotypes cannot be called with high certainty.
Results: We present a software tool, NgsRelate, for estimating pairwise relatedness from NGS data. It provides maximum likelihood estimates that are based on genotype likelihoods instead of genotypes and thereby takes the inherent uncertainty of the genotypes into account. Using both simulated and real data, we show that NgsRelate provides markedly better estimates for low-depth NGS data than two state-of-the-art genotype-based methods.
Availability: NgsRelate is implemented in C++ and is available under the GNU license at www.popgen.dk/software.
Contact: ida@binf.ku.dk
Supplementary information: Supplementary data are available at Bioinformatics online.
Filaments from the galaxy distribution and from the velocity field in the local universe
Authors: Libeskind Noam I. , Tempel Elmo , Hoffman Yehuda , Tully R. Brent , Courtois Hélène 
Published: September 2015

Abstract
The cosmic web that characterizes the large-scale structure of the Universe can be quantified by a variety of methods. For example, large redshift surveys can be used in combination with point process algorithms to extract long curvilinear filaments in the galaxy distribution. Alternatively, given a full 3D reconstruction of the velocity field, kinematic techniques can be used to decompose the web into voids, sheets, filaments and knots. In this Letter, we look at how two such algorithms – the Bisous model and the velocity shear web – compare with each other in the local Universe (within 100 Mpc), finding good agreement. This is both remarkable and comforting, given that the two methods are radically different in ideology and applied to completely independent and different data sets. Unsurprisingly, the methods are in better agreement when applied to unbiased and complete data sets, like cosmological simulations, than when applied to observational samples. We conclude that more observational data is needed to improve on these methods, but that both methods are most likely properly tracing the underlying distribution of matter in the Universe.
The Effects of Dexmedetomidine and Remifentanil on Hemodynamic Stability and Analgesic Requirement After Craniotomy: A Randomized Controlled Trial
Authors: Rajan Shobana , Hutcherson Matthew T. , Sessler Daniel I. , Kurz Andrea , Yang Dongsheng , Ghobrial Michael , Liu Jinbo , Avitsian Rafi 
Published: September 2015

Background:
Anesthesia for craniotomies should blunt responses to noxious stimuli, whereas subsequently leaving patients sufficiently alert for early neurological evaluation. The aim was to compare postoperative blood pressure control, pain, and opioid requirement after anesthesia with dexmedetomidine versus remifentanil. We therefore tested 2 primary hypotheses: (1) intraoperative administration of dexmedetomidine provides better control of postoperative blood pressure than remifentanil; and (2) patients given dexmedetomidine have less postoperative pain and use less opioid.

Materials and Methods:
Adults having elective brain tumor excisions under balanced general anesthesia with endotracheal intubation were randomized to an infusion of remifentanil (0.08 to 0.15 μg/kg/min, n=71) or dexmedetomidine (0.2 to 0.7 μg/kg/h, n=68). Patients also received propofol, rocuronium, fentanyl, and sevoflurane. The mean arterial pressure (MAP) and pain were recorded at 15, 30, 45, 60, and 90 postoperative minutes. Outcomes were assessed with joint hypothesis testing, evaluating noninferiority and superiority.

Results:
Compared with remifentanil, the use of dexmedetomidine was associated with reduced postoperative MAP (88±12 vs. 98±11 mm Hg), with estimated mean difference (97.5% confidence interval) of −10 (−13, −4) mm Hg, P<0.001, and mean visual analog pain score (2.9±2.6 vs. 5.1±2.4 points), with estimated mean difference of −5 (−10, −3) points, P<0.001, and required less median opioid consumption (5 [0, 10] vs. 10 [7, 15] mg morphine equivalents), with estimated median difference of −5 (−10, −3) mg, P<0.001. Dexmedetomidine was both noninferior and superior to remifentanil in maintaining postoperative hemodynamics and providing improved pain control.

Conclusions:
Intraoperative dexmedetomidine better controlled postoperative MAP and provided superior analgesia in patients undergoing craniotomy.
A Comparison of Macintosh and Airtraq Laryngoscopes for Endotracheal Intubation in Adult Patients With Cervical Spine Immobilization Using Manual In Line Axial Stabilization: A Prospective Randomized Study
Authors: Vijayakumar Vinodhadevi , Rao Shwethapriya , Shetty Nanda 
Published: September 2015

Background:
During cervical spine immobilization using Manual In Line Axial Stabilization (MILS), it is difficult to visualize the larynx by aligning the oropharyngeolaryngeal axes using Macintosh laryngoscope. Theoretically, Airtraq an anatomically shaped blade with endotracheal tube guide channel offers advantage over Macintosh. We hypothesized that intubation would be easier and faster with Airtraq compared with Macintosh laryngoscope.

Materials and Methods:
Ninety anesthetized adult patients with normal airways were intubated by experienced anesthesiologists after cervical immobilization with MILS either with Macintosh or Airtraq. Primary outcomes compared were successful intubation, and degree of difficulty of intubation as assessed by Intubation Difficulty Scale (IDS) score. Secondary outcomes compared were duration of laryngoscopy and intubation, degree of difficulty of intubation as assessed by Numerical Rating Scale score, soft tissue, and dental trauma.

Results:
All 90 patients were successfully intubated in the first attempt. Intubation as assessed by IDS score was easier in Airtraq (84.44%) in contrast to slight difficulty in the Macintosh (77.78%) group; Numerical Rating Scale score was easy in both the groups (Airtraq—91.12%; Macintosh—93.34%). The median (interquartile range [IQR]) time for laryngoscopy, (12 s [IQR, 8 to 17.5) vs. 8 s [IQR, 6 to 12]); total duration for intubation (25 s [IQR, 20-33] vs. 22 s [IQR, 18-27.5]) were prolonged in Airtraq group in comparison to Macintosh group.

Conclusions:
In anesthetized adult patients with MILS compared with Macintosh, Airtraq provides equal success rate of intubation, statistically significant (although clinically insignificant) longer duration for laryngoscopy and intubation. Intubation with Airtraq was significantly easier than Macintosh as assessed by the IDS score.
The Impact of Pelvic Floor and Lower Gastrointestinal Symptoms on Quality of Life in Women With Systemic Sclerosis
Authors: Umar Sarah B. , Griffing Leroy , Garcia Heidi , Foxx-Orenstein Amy E. , DiBaise John K. , Crowell Michael D. 
Published: September 2015

Background:
Systemic sclerosis (SSc) patients with gastrointestinal (GI) involvement have a lower quality of life (QoL) and while the impact of upper GI symptoms on QoL in SSc patients has been described few data exist on the presence and impact of lower gastrointestinal (LGI) and pelvic floor symptoms in SSc. Our goal was to assess the prevalence of these symptoms in women with SSc and evaluate their impact on QoL. A secondary hypothesis was that the impact of LGI symptoms on QoL is mediated by depression.

Study:
Women with SSc (n=175) attending an outpatient scleroderma clinic completed multiple validated questionnaires. Pelvic floor and LGI symptoms included fecal incontinence (FI), urinary incontinence (UI), dual incontinence (DI), chronic constipation, diarrhea, and pelvic pain. The Student t tests adjusted for multiple comparisons were used to evaluate group differences at the 0.05 level.

Results:
Complete data were available for 160 women. FI was reported by 65, UI by 64, DI by 40, chronic constipation by 94, diarrhea by 82, and pelvic pain by 35 of SSc patients. Overall QoL was reduced in SSc patients with FI (0.96 vs. 0.63; P=0.007), UI (0.96 vs. 0.65; P=0.01), DI (1.11 vs. 0.67; P=0.002), and pelvic pain (1.01 vs. 0.70; P=0.04). Antidepressant use was reported by 26%. The negative impact on QoL in patients with pelvic floor symptoms was partially mediated by depression.

Conclusions:
Women with SSc suffer from an increased prevalence of LGI and pelvic floor symptoms including FI, UI, diarrhea, constipation, and pelvic pain and this effect seems to be partially mediated by depression.
Health‐Related Quality of Life in Spanish Children With Coeliac Disease
Authors: Barrio Josefa , Román Enriqueta , Cilleruelo MaLuz , Márquez Manuela , Mearin MaLuisa , Fernández Cristina 
Published: September 2015

ABSTRACTObjectives:The aim of the study was to assess health‐related quality of life (HRQOL) using the Coeliac Disease Dutch Questionnaire (CDDUX) in Spanish children with coeliac disease.Methods:The CDDUX was cross‐culturally adapted according to international consensus guidelines. HRQOL was assessed in coeliac members of the Madrid Coeliac Association ages 8 to 18 years using the adapted CDDUX. Cronbach α coefficient was determined as a measure of intraquestionnaire reliability and intraclass correlation coefficients as a measure of reliability between scores awarded by children and parents. Demographic and clinical variables associated with HRQOL were also assessed.Results:A total of 1602 children of 3122 registered Madrid Coeliac Association members ages 8 to 18 years were invited to participate. The questionnaire was completed by 480 families (30%): 214 only by parents, 214 by parents and their children, and 52 only by children. Cronbach α coefficient for the total score for parents was 0.90, and for children 0.88 (0.75–0.90 by scales). Mean total (standard deviation [SD]) HRQOL scores in children and parents were 55.5 (SD 12.7) and 53.89 (SD 12.19), respectively, with no differences detected in paired comparisons between the 2 groups. Significantly worse HRQOL scores were recorded in children showing a nonclassical clinical presentation, in those not adhering to treatment and in those reporting difficulties in following the diet.Conclusions:The CDDUX questionnaire emerged as reliable for use in Spanish children with celiac disease. Overall, both children and parents felt the disease had no substantial negative impacts on patient HRQOL.
Prevalence of Anterior Cutaneous Nerve Entrapment Syndrome in a Pediatric Population With Chronic Abdominal Pain
Authors: Siawash Murid , de Jager‐Kievit Jenneke W.A. , Ten Walther Tjon A. , Roumen Rudi M. , Scheltinga Marc R. 
Published: September 2015

ABSTRACTObjective:Anterior cutaneous nerve entrapment syndrome (ACNES) is a frequently overlooked condition causing chronic abdominal pain (CAP). The objective of the present study was to investigate the rate of ACNES in a pediatric outpatient cohort with CAP.Methods:A cross‐sectional cohort study was conducted in a population 10 to 18 years of age consulting a pediatric outpatient department with new‐onset CAP during a 2 years’ time period. All individuals were identified through a standard hospital registration system. History, physical examination, diagnosis, and success of treatment were obtained in patients who were diagnosed as having ACNES.Results:Twelve of 95 adolescents with CAP were found to be experiencing ACNES. Carnett sign was positive at the lateral border of the rectus abdominus muscle in all 12. Altered skin sensation was present in 11 of 12 patients with ACNES. Six weeks after treatment (1–3 injections, n = 5; neurectomy, n = 7), pain was absent in 11 patients.Conclusions:ACNES is present in 1 of 8 adolescents presenting with CAP to a pediatric outpatient department of a teaching hospital. Simple physical examinational testing allows for the diagnosis. Treatments including nerve blocks or surgery are beneficial in most.
Standardizing Falls Reporting: Using Data From Adverse Event Reporting to Drive Quality Improvement
Authors: Gardner Lea Anne , Bray Phyllis J. , Finley Edward , Sterner Carly , Ignudo Thomas L. , Stauffer Cherie L. , Kincaid Shawn A. , Marella William M. 
Published: September 2015

Objective
To enhance the value of the Pennsylvania Patient Safety Reporting System (PA-PSRS) falls reports by developing a falls reporting program that standardizes falls reporting and provides timely benchmarked falls rates and process measurement reports hospitals can use to identify areas of improvement in their falls program.

Methods
The new PA-PSRS falls reporting program requires adherence to standardized definitions of falls to generate standardized, customizable analytic reports. An advisory committee and statewide survey guided the development of the program, data definitions, system features and functionality, and methods for stratifying reporting criteria.

Results
Three real-time falls outcomes and 2 falls process reports with multiple configurable options were created. A falls dashboard was developed based on gaps in falls risk assessment processes identified in PA-PSRS falls event reports. Six months after launching the program, 41.3% of Pennsylvania hospitals enrolled. The Authority's annual survey indicated 82.9% of participating hospitals found that the new falls analytic reports were somewhat useful to very useful. Preliminary impact on the falls with harm rate has been limited, a less than 1% reduction, but the ability to identify specific organizational and patient risk factors in a timely manner provides hospitals with opportunities to target falls prevention resources more effectively.

Conclusions
The PA-PSRS falls reporting program's standardized definition of falls offers new analytic reports that include falls rates with benchmarking data and a falls dashboard. The benchmarking data allow hospitals to compare themselves to peer hospitals statewide. The newly expanded PA-PSRS falls reporting program has turned an adverse event-reporting program into a quality improvement tool.