bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of miRNA signatures for ovarian cancer earlier detection in the pre-diagnosis setting using machine learning approaches
Authors: Stawiski Konrad , Fortner Renée T. , Pestarino Luca , Umu Sinan U. , Kaaks Rudolf , Rounge Trine B. , Elias Kevin M. , Fendler Wojciech , Langseth Hilde 
Published: June 2024

IntroductionEffective strategies for early detection of epithelial ovarian cancer are lacking. We evaluated whether a panel of 14 previously established circulating microRNAs could discriminate between cases diagnosed &lt;2 years after serum collection and those diagnosed 2–7 years after serum collection. miRNA sequencing data from subsequent ovarian cancer cases were obtained as part of the ongoing multi-cancer JanusRNA project, utilizing pre-diagnostic serum samples from the Janus Serum Bank and linked to the Cancer Registry of Norway for cancer outcomes.MethodsWe included a total of 80 ovarian cancer cases contributing 80 serum samples and compared 40 serum samples from cases with samples collected &lt;2 years prior to diagnosis with 40 serum samples from cases with sample collection ≥2 to 7 years. We employed the extreme gradient boosting (XGBoost) algorithm to train a binary classification model using 70% of the available data, while the model was tested on the remaining 30% of the dataset.ResultsThe performance of the model was evaluated using repeated holdout validation. The previously established set of miRNAs achieved a median area under the receiver operating characteristic curve (AUC) of 0.771 in the test sets. Four out of 14 miRNAs (hsa-miR-200a-3p, hsa-miR-1246, hsa-miR-203a-3p, hsa-miR-23b-3p) exhibited higher expression levels closer to diagnosis, consistent with the previously reported upregulation in cancer cases, with statistical significance observed only for hsa-miR-200a-3p (beta=0.14; p=0.04). DiscussionThe discrimination potential of the selected models provides evidence of the robustness of the miRNA signature for ovarian cancer.
PpSAUR5 promotes plant growth by regulating lignin and hormone pathways
Authors: Li Xin-Miao , Zhai Han-Han , An Xiu-Hong , Zhang He , Zhang Xueying , Wang Pengfei , Chen Haijiang , Tian Yi 
Published: June 2024

IntroductionPeach (Prunus persica) has a high nutritional and economic value. However, its overgrowth can lead to yield loss. Regulating the growth of peach trees is challenging. The small auxin-up RNA (SAUR) gene family is the largest family of auxin-responsive genes, which play important roles in plant growth and development. However, members of this gene family are rarely reported in peach.MethodsIn this study, we measured leaf area, chlorophyll and lignin content to detect the role of PpSAUR5 on growth through transgenic Arabidopsis.ResultsPpSAUR5 responds to auxin and gibberellin, promoting and inhibiting the synthesis of gibberellin and auxin, respectively. The heterologous transformation of PpSAUR5 in Arabidopsis led to enhanced growth of leaves and siliques, lightening of leaf color, decrease in chlorophyll content, increase in lignin content, abnormalities in the floral organs, and distortion of the inflorescence axis. Transcriptome data analysis of PpSAUR5 overexpression and wild-type lines revealed 854 differentially expressed genes (DEGs). GO and KEGG analyses showed that the DEGs were primarily involved in biological processes, such as cellular processes, metabolic processes, response to stimuli, and catalytic activity. These genes were mainly enriched in pathways, such as phenylalanine biosynthesis, phytohormone signaling, and MAPK signaling.DiscussionIn summary, these results suggested that PpSAUR5 might regulate tree vigor by modulating the synthesis of auxin and gibberellin. Future studies can use PpSAUR5 as a candidate gene to elucidate the potential regulatory mechanisms underlying peach tree vigor.
Development of a 45K pepper GBTS liquid-phase gene chip and its application in genome-wide association studies
Authors: Li Zixiong , Jia Zhiqi , Li Jisuo , Kang Dongmu , Li Mingxuan , Ma Shijie , Cheng Qing , Shen Huolin , Sun Liang 
Published: June 2024

IntroductionPepper (Capsicum spp.) is a vegetable that is cultivated globally and has undergone extensive domestication, leading to a significant diversification in its agronomic traits. With the advancement of genomics in pepper and the reduction in sequencing costs, the high-throughput detection of single nucleotide polymorphisms (SNPs) and small insertions-deletions (indels) has become increasingly critical for analyzing pepper germplasms and improving breeding programs. As a result, there is a pressing need for a cost-effective, high-throughput, and versatile technique suitable for both foreground and background selection in pepper breeding.MethodsIn the present study, Python-based web scraping scripts were utilized to systematically extract data from published literatures and relevant sequence databases focusing on pepper genomes. Subsequent to data extraction, SNPs and indels were meticulously identified and filtered. This process culminated in the delineation of core polymorphic sites, which were instrumental in the development of specific probes. Following this, comprehensive phenotypic and genotypic analyses were conducted on a diverse collection of 420 pepper germplasms. Concurrently, a genome-wide association study (GWAS) was conducted to elucidate the genetic determinants of helical fruit shape in peppers.ResultsIn this study, a 45K pepper Genotyping-By-Target-Sequencing (GBTS) liquid-phase gene chip was developed on the GenoBaits platform. This chip is composed of 45,389 probes, of which 42,535 are derived from core polymorphic sites (CPS) in the background genetic landscape, while 2,854 are associated with foreground agronomic traits, spanning across 43 traits. The CPS probes are spaced at an average interval of 68 Kb. We have assessed the performance of this chip on 420 pepper germplasms, with successful capture of target DNA fragments by 45,387 probes. Furthermore, the probe capture ratio surpassed 70% in 410 of the 420 germplasms tested. Using this chip, we have efficiently genotyped 273 germplasms for spiciness levels and elucidated the genetic relationships among 410 pepper germplasms. Our results allowed for precise clustering of sister lines and C. chinense germplasms. In addition, through a GWAS for helical fruit shape, we identified three quantitative trait loci (QTLs): heli2.1, heli11.1, and heli11.2. Within the heli11.1 QTL, a gene encoding the tubulin alpha chain was identified, suggesting its potential role in the helical growth pattern of pepper fruits.DiscussionIn summary, the 45K pepper GBTS liquid-phase gene chip offers robust detection of polymorphic sites and is a promising tool for advancing research into pepper germplasm and the breeding of new pepper varieties.
Strain and serenity: exploring the interplay of stress, burnout, and well-being among healthcare professionals
Authors: Dobešová Cakirpaloglu Simona , Cakirpaloglu Panajotis , Skopal Ondřej , Kvapilová Barbora , Schovánková Tereza , Vévodová Šárka , Greaves Jane Peta , Steven Alison 
Published: June 2024

IntroductionStress and burnout can negatively affect performance, mental health, and the overall well-being of healthcare workers. The study aims to examine the prevalence of stress and burnout, and investigate links between stress, burnout, mental state, and well-being among healthcare workers in the Czech Republic.MethodsA cross-sectional survey was conducted in the Czech Republic, focusing on healthcare professionals working in various healthcare settings. A total of 1,064 healthcare workers participated in the study. A standardized questionnaire battery was used, consisting of the Maslach Burnout Inventory (MBI), Perceived Stress Scale (PSS) and Supso-7 measuring mental state. Separate correlation and multiple regression analyses were conducted.Results46.24% of the healthcare workers reported high levels of emotional exhaustion, 25.56% reported high levels of depersonalization, 24.15% reported low levels of personal accomplishment, while 11.18% reported high levels of perceived stress. The findings revealed that emotional exhaustion, a core component of burnout, was associated with increased feelings of anxiety and depression. Perceived stress was also linked to anxiety and depression, while personal accomplishment appeared to mitigate depression and support positive psychological well-being.ConclusionThe study provides promising evidence suggesting that addressing stress and emotional exhaustion, while fostering a sense of personal achievement, could lead to improvements in the mental health and work performance of healthcare workers. These findings highlight the importance of addressing burnout and stress management strategies to support the overall well-being of healthcare professionals.
Comparative analysis and evolution of civilian versus combatant mortality ratios in Israel-Gaza conflicts, 2008–2023
Authors: Ayoub Houssein H. , Chemaitelly Hiam , Abu-Raddad Laith J. 
Published: June 2024

BackgroundThere is a need for statistical methodologies that scrutinize civilian casualties in conflicts, evaluating the degree to which the conduct of war affects civilians and breaches the laws of war. Employing an epidemiological method, this study introduced, developed, and applied a novel approach for investigating mortality of civilians versus combatants in conflicts.MethodsA deterministic mathematical model, structured by age and sex, was developed to describe the process of conflict-related deaths among both combatants and civilians. The model was calibrated using demographic and conflict-related data from different Israel-Gaza conflicts. To quantify the extent of the impact on civilians and determine whether they are the primary focus of a conflict, a statistical metric, the index of killing civilians, along with associated criteria, was devised.ResultsThe model-estimated proportion of deaths in Gaza categorized as combatants was 62.1% (95% uncertainty interval (UI): 57.6–66.2%), 51.1% (95% UI: 47.1–54.9%), and 12.7% (95% UI: 9.7–15.4%) in the 2008–2009, 2014, and 2023 Israel-Gaza conflicts, respectively. The index of killing civilians was 0.61 (95% UI: 0.51–0.74), 0.96 (95% UI: 0.82–1.12), and 7.01 (95% UI: 5.50–9.29) in the 2008–2009, 2014, and 2023 conflicts, respectively. These index values indicate strong evidence for civilians being an object of war in the 2008–2009 and 2014 conflicts, but combatants were still identified as the primary focus of the conflict. In the 2023 conflict, there is robust evidence for civilians being an object of war, with civilians identified as the primary focus of the conflict.ConclusionFindings imply a progressive shift in Israel’s rules of engagement over time, with a trend towards higher acceptance of casualties among civilians. The 2023 conflict stands apart from preceding Israel-Gaza conflicts, with civilians identified as the primary focus of the conflict.
Growth mindset and well-being in social interactions: countering individual loneliness
Authors: Wang Chao , Li Shanshan , Wang Yilin , Li Mengxia , Tao Weidong 
Published: June 2024

IntroductionLoneliness is a prevalent negative emotion experienced by college students. This study explores the relationship between a growth mindset and loneliness among college students.MethodsA total of 560 college students completed the Growth Mindset Scale (GMS), UCLA Loneliness Scale (UCLA), Interpersonal Relationships Assessment Scale (IRS), and two measures assessing distinct facets of well-being the Satisfaction with Life Scale (SWLS) and the revised Positive Affect and Negative Affect Scale (PANAS).Results and discussionThe results found a significant negative correlation between a growth mindset and loneliness. A growth mindset negatively predicted loneliness through the chain-mediated effects of interpersonal distress and well-being. These findings underscore the important role of a growth mindset in influencing loneliness, providing teachers and practitioners a new perspective to understand and intervene college students’ psychological challenges.
Age and estimated glomerular filtration rate in Chinese older adults: a cohort study from 2014 to 2020
Authors: Jiang Ying , Cao Qin , Hong Weiqi , Xu Tianwei , Tang Molian , Li Yun , Xu Renying 
Published: June 2024

ObjectivesThis study aimed to fill the data gap of the course of renal function decline in old age and explore changes in renal function across different health states with increasing age.MethodsThis observational, retrospective, single-center cohort study included 5,112 Chinese older adults (3,321 men and 1,791 women, range 60–104 years). The individual rate of estimated glomerular filtration rate (eGFR) decline was analyzed using linear mixed-effects model to account for repeated measures over the years.ResultsThe median age was 66 years, median BMI was 24.56 kg/m2, and median eGFR was 89.86 mL/min.1.73 m2. For every 1-year increase in age, women’s eGFR decreased by 1.06 mL/min/1.73 m2 and men’s by 0.91 mL/min/1.73 m2. We observed greater age-related eGFR decline in men and women with high systolic blood pressure (SBP). Men with high triglyceride (TG), high low-density lipoprotein cholesterol (LDL-C), and low high-density lipoprotein cholesterol (HDL-C), had greater age-related eGFR decline. In women, different BMI groups showed significant differences in age-related eGFR decline, with the highest decline in those with obesity. Additionally, participants with normal baseline eGFR had a faster age-related decline than those with low baseline eGFR.ConclusionThe eGFR declined linearly with age in Chinese older adults, with women exhibiting a slightly faster decline than men. Both men and women should be cautious of SBP. Older adults with normal baseline renal function experienced a faster eGFR decline. Men with high TG, LDL-C, and low HDL-C levels, as well as obese women, should be vigilant in monitoring renal function.
Upper tract access in patients with urinary diversions
Authors: Modai Jonathan , Bhanji Yasin , Patel Parth M. , Scotland Kymora , Dunn Matthew D. 
Published: June 2024

Urinary tract diversions are a common reconstructive solution for patients with malignant, anatomic or functional pathologies of the lower urinary tract. Although urinary diversions often represent the patient’s best alternative, they are not devoid of complications. Some of these complications, mainly anastomotic strictures and kidney stones, can be managed endoscopically, although upper tract access of such patients is not straightforward, requiring a fundamental understanding of the many types of diversions. In this article we will review the inherent difficulties of accessing the upper tract of patient with different diversions, the different approaches to the upper tract of diverted patients, and the equipment and techniques that can help facilitate upper tract access in diverted patients.
A retrospective study on the efficacy of the ERAS protocol in patients who underwent laparoscopic left and right colectomy surgeries
Authors: Zhao Xuemei , Jin Siyu , Peng Mingxiu , Wang Jingjing 
Published: June 2024

ObjectiveRetrospective analysis and comparison of the effects of Enhanced Recovery After Surgery (ERAS) protocol for patients having left and right colectomy surgeries.MethodOut of the patients admitted to Chengdu Shang Jin Nan Fu Hospital and West China Hospital from December 2019 to December 2022, a total of 498 who met the inclusion criteria were selected, 255 with right colectomy(RC) and 243 with left colectomy (LC). Under the conditions of strict compliance with ERAS protocol, the relevant physical indexes of RC and LC, including postoperative rehabilitation (especially median post-operative stay) and complications (especially prolonged postoperative ileus, PPOI), were statistically analyzed and compared.ResultsIn terms of intraoperative variables, fluid doses were higher in the LC group than in the RC group (P &lt; 0.05), and there was no significant difference between them in terms of operative time, blood loss, need for open surgery, peritoneal contamination, epidural catheter placement, or opioid use (P &gt; 0.05). Compared with the RC group, the LC group had a higher intake of oral liquid at the second postoperative day (POD), and faster first flatulence (P &lt; 0.05). 30 (11.76%) RC patients required nasogastric tube insertion, while only 3 (1.23%) patients in the LC group required the same (P &lt; 0.05). Prolonged postoperative ileus (PPOI) occurred in 48 (18.82%) and 29 (11.93%) patients in the RC and LC groups, respectively (P &lt; 0.05). No significant differences in terms of postoperative complications or length of hospital stay (LoS). stay were observed.ConclusionAs the location of colon cancer changes, the effectiveness of ERAS also varies. More personalized and precise ERAS protocols can reduce the incidence of postoperative complications and promote rapid recovery after surgery.
La legalización de la eutanasia en el Ecuador, desafíos desde una perspectiva comparada
Authors: Armijos Armijos Jhovanna Mirlay , Maldonado Ruiz Luis Mauricio 
Published: June 2024

La investigación aborda cuestiones esenciales frente a la búsqueda de la despenalización de la eutanasia, que se espera sirva como fuente investigativa para su implementación en el marco jurídico en el Ecuador y no simplemente como precedente jurisprudencial. La Constitución tiene los elementos necesarios para reconocer una muerte digna como un derecho por ser un Estado Constitucional de Derechos y de Justicia, para ello, la metodología empleada en esta investigación es el método del derecho comparado externo porque permite comparar ordenamientos jurídicos extranjeros con el objetivo de destacar las semejanzas de cada uno de ellos y verificar que la legalización de la eutanasia no es un problema jurídico sino que ayuda para mantenernos en la línea de la protección de derechos humanos, por ello para su análisis hemos considerado comparar los ordenamientos jurídicos de España, Colombia, Holanda, Bélgica y el Estado de Oregón, ya que han sido pioneros en la lucha del reconocimiento a una muerte digna pese a poseer culturas jurídicas diferentes. En resumen, todo esto nos proporciona una visión valiosa para explorar este tema complejo con la finalidad de darle proponer una solución que contribuya en garantizar los derechos individuales desde la toma de decisiones en Ecuador.
Lucio Fontana, le Spatialisme, et les néo-avant-gardes en Italie autour de 1950
Authors: Joppolo Giovanni 
Published: June 2024

Lucio Fontana, der Spazialismo und die Neo-Avantgarde in Italien in den 1950 Jahren.
Der Artikel setzt sich mit der Rekonstruktion der Geschichte des Spazialismo auseinander und mit der zentralen Rolle, die der italo-argentinische Künstler Lucio Fontana einnahm, der diese Bewegung zwischen Buenos Aires und Mailand gründete. Der hier gewählte methodische Ansatz berücksichtigt die neueren Beiträge der Historiographie, die Lucio Fontana und den italienischen Neo-Avantgardisten der Nachkriegszeit gewidmet sind. Es wird ein chronologischer Index der Manifeste und der programmatischen Texte der Bewegung des Spazialismo beigefügt, der die Jahre 1946-1958 umfaßt.
Le nu historié : les envois des pensionnaires de l'Académie de France à Rome au XVIIIe siècle
Authors: Bédard Sylvain 
Published: June 2024

Der historisierte Akt. Die nach Paris geschickten Werke der Stipendiaten der französischen Akademie in Rom im 18. Jahrhundert
In den Jahren von 1754 bis 1792 waren die Maler der französischen Akademie in Rom dazu angehalten, einmal im Jahr - neben anderen Studienarbeiten - eine Aktstudie auf Leinwand nach Paris zu schicken. Dies ging auf die Initiative des Malers Charles-Joseph Natoire zurück, der bis 1775 Direktor der römischen Institution war. In Paris angekommen, wurden diese Werke einer Jury vorgestellt, die sich aus Beamten der königlichen Akademie zusammensetzte. Ihr Bericht wurde umgehend nach Rom geschickt, wo die Stipendiaten Kenntnis von den Kommentaren der Panser Richter nahmen. Diese Studie bemüht sich, einen Überblick über diese Akte zu verschaffen, von denen heute eine bestimmte Anzahl lokalisiert ist. Außerdem soll die Ambition ihrer Autoren analysiert werden, über die einfache Studie nach dem lebenden Modell hinausgehen zu wollen, um ein Historien-Sujet zu erstreben.
Peut-on parler d'une sculpture d'histoire ? Essai comparatif sur les monuments aux grands hommes en France et en Italie au XIXe siècle
Authors: Chevillot Catherine 
Published: June 2024

Is there such a thing as a history sculpture ? A comparative essay on the monuments to great men in 19th century France and Italy.
Numerous studies have shown the extent to which sculpture, to an even greater degree than painting, was linked to ideologies and therefore to the interpretation of history. To question the validity of the term "history sculpture" can seem incongruous. Yet, public commemorative monuments rarely veer out of the field of portraiture. The present essay seeks to study the motives and consequences of such visual and iconographical choices by focusing on two countries, Italy and France. Both experienced, after 1870, a significant increase in public commemorations, most notably paying tribute to recent events. This paper will more particularly examine the reluctance of representing recent events, the relationship between sculptors, patrons and municipal officials, and the fraught coexistence between effigy and simulacrum in the field of realism.
"Pittura di luce" : genèse d'une notion
Authors: Rowley Neville 
Published: June 2024

"Pittura di luce" : genèse d'une notion.
Cet article constitue une vaste enquête sur les fondements historiographiques d'un mouvement pictural du Quattrocento florentin, la pittura di luce, qui n'a été baptisé ainsi qu'en 1990. Son intérêt premier est de démontrer que ce cornant fut perçu par la critique d'art, principalement italienne, bien avant de se trouver un nom. Plus que tout autre historien, Roberto Longhi aura contribué de manière décisive à l'éclaircissement d'une telle notion : d'abord en identifiant, dès 1914, un mouvement "lumineux" italien, placé sous l'égide de Piero della Francesca, puis en s'attachant, en 1952, à sa spécificité florentine autour de la figure de Domenico Veneziano. Près de deux décennies après avoir été nommée, la "peinture de lumière" apparaît aujourd'hui comme l'un des thèmes majeurs d'une étude renouvelée de la première Renaissance italienne.
Monstrum mirabile dictum : ironia e grottesco nell'arte di Asger Jorn ed Enrico Baj
Authors: Di natale Giuseppe 
Published: June 2024

Monstrum mirabile dictum : l'ironie et le grotesque dans l'art d'Asger Jorn et Enrico Baj.
L'article se propose d'analyser les motivations d'Enrico Baj et Asger Jorn et les outils qu'ils ont choisi pour donner à leurs œuvres un caractère ironico-grotesque, en cherchant à mettre en évidence le sentiment d'inquiétude qui, grâce à une ironie parfois féroce ou à un subtil processus de distorsion, met en doute la perception même que peut en avoir le spectateur. Si l'ironie de Baj se réfère à une forme de rébellion contre la société de consommation, le pouvoir, la bêtise de la bourgeoisie et sa vulgarité, les matériaux qu'il utilise deviennent plutôt le medium à travers lequel l'artiste met au point sa critique, en se servant de quelques objets symboliques de référence vers lesquels la critique elle-même s'était tournée. Asger Jorn, par contre, a fait de l'ironie un trait stylistique capable de mettre l'accent sur les incohérences du monde et de l'art grâce à la mise en œuvre d'un processus complexe de dissimulation et de mystification. Les implications philosophiques et littéraires quant à elles sont diverses, de Søren Kierkegaard à Franz Kafka, en passant par la pataphysique d'Alfred Jarry à laquelle ont adhéré les deux artistes au cours des années soixante.