bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oral Cancer Diagnosis via a Ferrocenylnaphthalene Diimide–Based Electrochemical Telomerase Assay
Authors: Mori Kumiko , Sato Shinobu , Kodama Masaaki , Habu Manabu , Takahashi Osamu , Nishihara Tatsuji , Tominaga Kazuhiro , Takenaka Shigeori 
Published: November 2012

BACKGROUND
Telomerase is regarded as a good marker for cancer because it is unregulated in most tumor cells compared with normal cells. We evaluated telomerase activity in the lysate of tumor tissue and surrounding cells of oral cancer patients by an electrochemical technique, dubbed the electrochemical telomerase assay (ECTA).

METHODS
The assay used ferrocenylnaphthalene diimide (FND) as the probe. Electrochemical telomerase substrate (ETS) primer immobilized on the electrode was elongated by telomerase and FND bound to the product to give rise to a current. The data were standardized with the change in current before and after the elongation, respectively.

RESULTS
The change in current increased more than 30% in biopsy samples from most cancer patients, whereas the increase was 20% or lower in most healthy individuals. On the basis of this difference individual clinical samples were judged telomerase positive, ambiguous, or negative. The positive rates in the cancerous tissues and exfoliated cells (EOCs) of the patients were 85% and 90%, respectively, whereas the corresponding values were 50% and 10% by the telomerase repeat amplification protocol. Furthermore, the positive rate for the ECTA was 100% in early tumors smaller than 2 cm, and 95% and 82% of biopsy and exfoliated cells of healthy individuals were correctly judged as negative. Fifty-six unknown samples with EOCs tested were correctly judged to be cancerous or normal in 84% of cases.

CONCLUSIONS
The ECTA yielded high hit rates for cancerous and normal cells, especially in EOCs, results indicating that this minimally invasive test is suitable for oral cancer diagnosis.
Copy Number and Expression Alterations of miRNAs in the Ovarian Cancer Cell Line OVCAR-3: Impact on Kallikrein 6 Protein Expression
Authors: Bayani Jane , Kuzmanov Uros , Saraon Punit , Fung William A , Soosaipillai Antoninus , Squire Jeremy A , Diamandis Eleftherios P 
Published: November 2012

BACKGROUND
Kallikrein-related peptidase 6 (KLK6), a member of the serine protease family of kallikrein (KLK) genes, is dysregulated in ovarian carcinomas (OCa) and its overexpression is associated with poor prognosis. Regulation of its expression is poorly understood and is likely to be influenced by multiple mechanisms. The KLK locus is subject to copy number changes and heterogeneity in serous OCas. These copy number imbalances generally correlate with KLK6 protein expression; however, this is not always the case. In this study we explored the role of miRNAs in the posttranscriptional control of KLK6 expression and the contributions of copy numbers, not only of the KLK locus, but also of the miRNAs predicted to regulate it.

METHODS AND RESULTS
By miRNA profiling of the KLK6-overexpressing OCa cell line, OVCAR-3, we identified overexpressed and underexpressed miRNAs. Publically available miRNA databases identified the human miRNA lethal 7 (hsa-let-7) family members as putative regulating miRNAs, from which hsa-let-7a was chosen for functional analysis. The transient transfection of hsa-let-7a to OVCAR-3 resulted in a decrease of KLK6 secreted protein. Moreover, such transfection was also able to weakly affect the expression of another member of the KLK gene family, KLK10 (kallikrein-related peptidase 10). Cytogenomic analysis, including array comparative genomic hybridization, fluorescence in situ hybridization, and spectral karyotyping revealed the overall net copy number losses of hsa-let-7a and other miRNAs predicted to target KLK6.

CONCLUSIONS
The hsa-let-7 family member hsa-let-7a is a modulator of KLK6 protein expression that is independent of the KLK6 copy number status.
ExactDAS: An Exact Test Procedure for the Detection of Differential Alternative Splicing in Microarray Experiments
Authors: Mary-Huard Tristan , Jaffrezic Florence , Robin Stéphane 
Published: November 2012

Abstract
The aim of this paper is to propose a test procedure for the detection of differential alternative splicing across conditions for tiling array or exon chip data. While developed in a mixed model framework, the test procedure is exact (avoiding computational burden) and applicable to a large variety of contrasts, including several previously published ones. A simulation study is presented to evaluate the robustness and performance of the method. It is found to have a good detection power of genes under differential alternative splicing, even for five biological replicates and four probes per exon. The methodology also enables the comparison of various experimental designs through exact power curves. This is illustrated with the comparison of paired and unpaired experiments. The test procedure was applied to two publicly available cancer data sets based on exon arrays, and showed promising results.
Large-scale Parentage Inference with SNPs: an Efficient Algorithm for Statistical Confidence of Parent Pair Allocations
Authors: Anderson Eric C. 
Published: November 2012

Abstract
Advances in genotyping that allow tens of thousands of individuals to be genotyped at a moderate number of single nucleotide polymorphisms (SNPs) permit parentage inference to be pursued on a very large scale. The intergenerational tagging this capacity allows is revolutionizing the management of cultured organisms (cows, salmon, etc.) and is poised to do the same for scientific studies of natural populations. Currently, however, there are no likelihood-based methods of parentage inference which are implemented in a manner that allows them to quickly handle a very large number of potential parents or parent pairs. Here we introduce an efficient likelihood-based method applicable to the specialized case of cultured organisms in which both parents can be reliably sampled. We develop a Markov chain representation for the cumulative number of Mendelian incompatibilities between an offspring and its putative parents and we exploit it to develop a fast algorithm for simulation-based estimates of statistical confidence in SNP-based assignments of offspring to pairs of parents. The method is implemented in the freely available software SNPPIT. We describe the method in detail, then assess its performance in a large simulation study using known allele frequencies at 96 SNPs from ten hatchery salmon populations. The simulations verify that the method is fast and accurate and that 96 well-chosen SNPs can provide sufficient power to identify the correct pair of parents from amongst millions of candidate pairs.
Phototactic responses of Elachista antarctica (Phaeophyceae) spores of different ages across a broad irradiance range using new motion analysis software
Authors: Bucolo Philip , Amsler Charles D. , McClintock James B. , Baker Bill J. 
Published: November 2012

Abstract
Phototactic movements of Elachista antarctica spores were quantified across a wide range of irradiances from 10 to 800 μmol photons m-2 s-1 using the recently updated version of CellTrak 1.5 motion analysis software. Spores 0–12 h old exhibited phototactic attraction at 80 and 200 μmol photons m-2 s-1, which contributed to vertical movements up out of the boundary layer to be further dispersed hydrodynamically, but were repelled at 800 μmol photons m-2 s-1, causing spores to retreat from detrimental irradiances. No significant effects were observed among spores released 24–36 h before measuring phototaxis at 200 and 800 μmol photons m-2 s-1.
On embedding left-ordered groups into division rings
Authors: Gräter Joachim , Sperner Robert P. 
Published: November 2012

Abstract
We present and discuss methods for embedding a left-ordered group G into the multiplicative group of a skew
field D. Among others, we are interested in the case in which any group automorphism of G extends
to a ring automorphism of D and we will be able to apply Dubrovin's embedding theorem to a special class
of left-ordered groups which we call locally complete.
Growth Directions of Primary Phases in Directionally Solidified Ti-45Al-7Nb Alloys
Authors: Yan Jie , Zheng Lijing , Zhang HuaRui , Xiao Zhixia , Zhang Hu 
Published: November 2012

AbstractTi-45Al-7Nb alloys are produced by liquid-metal-cooling (LMC) directional solidification (DS) furnace with different temperature gradients (G) at a range of 40–80 K/cm. The growth directions of primary β phases and the deflection angles relative to the 〈100〉β direction were investigated. The solidification pathway of alloy is: Liq. → Liq. + β → β → β + α → α + γ → lamella (α2 + γ) + B2. Primary dendrite arm spacing λ reduced from 611.4 µm to 508.7 µm when G increased from 40 K cm−1 to 80 K cm−1. Grains with lamellar orientation aligned at the angles of 12–15° and 55° to growth direction were favored for various G. The orientations of the γ lamellas in steady-state regions were identified by the electron backscattered diffraction analysis (EBSD). The results indicated that the growth directions of primary bcc-β dendrites were 〈211〉β and 〈110〉β at 40 K/cm, 〈210〉β and 〈211〉β at 50 K/cm and 〈211〉β at 80 K/cm, the deflection angle of 〈211〉β relative to 〈100〉β direction is 35.3°.
Numerical Simulation of the Strength and Plasticity Performance in Austenitic Stainless Steel
Authors: Li Dongsheng , Dai Qixun , Cheng Xiaonong 
Published: November 2012

AbstractThe effect of temperature and alloy content on the strength and plasticity of austenitic stainless steel was investigated in this paper. A series of expressions for quantitative calculation via computer were obtained according to the experimental data and are shown as
;
;
;
;
. The obtained results were reliable and can be applied to the design and calculation of the high-temperature strength and plasticity of austenitic stainless steel.
Effect of Aluminum on the Nucleation of Intragranular Ferrite in Ti-Added Carbon Structural Steel
Authors: Hui Kong , Shao Shuai , Shen YunFu , Zhou YaHui , Yue Qiang , Zhang JiuFeng , Cai ZhengYu 
Published: November 2012

AbstractThe features of inclusion and microstructure for Ti-added carbon structural steel were studied through the scanning electron microscope (SEM) and Energy Dispersive Spectrometer (EDS). From the SEM-EDS mapping images, two kinds of titanium-contained oxides are observed. The type I is the Al-Ti-O phase, in which the elements of Al and Ti coexist. The type II is the titanium-contained oxides, which precipitate on the Al2O3. Through the statistical analysis, it can be seen that the former type is dominating in number and smaller in size. After etching, the microstructure of intragranular acicular ferrites (IAF) is observed, which is totally different from that in the comparison sample without Ti addition. Furthermore, it’s found that the type I inclusion can act as nucleation sites for the IAF. However, the similar nucleation phenomenon is complex for the type II inclusion. This complexity is attributed to the diversity of precipitation of Ti and Mn.
Log-Sine Evaluations of Mahler Measures, II
Authors: Borwein David , Borwein Jonathan M. , Straub Armin , Wan James 
Published: November 2012

Abstract.We continue the analysis of higher and multiple Mahler measures using log-sine integrals as started in “Log-sine evaluations of Mahler measures” and “Special values of generalized log-sine integrals” by two of the authors. This motivates a detailed study of various multiple polylogarithms and worked examples are given. Our techniques enable the reduction of several multiple Mahler measures, and supply an easy proof of two conjectures by Boyd.
Numerical Solution of Time Fractional Navier-Stokes Equation by Discrete Adomian decomposition method
Authors: Birajdar Gunvant A. 
Published: November 2012

Abstract
In this paper we find the solution of time fractional
discrete Navier-Stokes equation using Adomian decomposition
method. Here we discretize the space domain.
The graphical representation of solution given by
using Matlab software, and it compared with exact solution
for alpha = 1.
Association of proinsulin and hepatic steatosis in a random, population-based sample
Authors: Wengert Sonja , Oeztuerk Suemeyra , Haenle Mark Martin , Koenig Wolfgang , Imhof Armin , Boehm Bernhard Otto , Wilhelm Manfred , Mao Ren , Mason Richard Andrew , Kratzer Wolfgang , _ _ 
Published: November 2012

ObjectiveProinsulin may represent a predictive marker for assessing insulin resistance and reduced β-cell function. The objective of this study was to investigate the association between hepatic steatosis, proinsulin and other parameters in a random, population-based sample.DesignCross-sectional study, conducted in south-western Germany.MethodsUpper abdominal ultrasound examinations were performed in 343 subjects (147 females, 196 males; average age 40.0±11.5 years). Proinsulin, the proinsulin-to-insulin ratio and other laboratory parameters were determined, and the BMI, waist-to-hip ratio (WHR) and other anthropometric data were documented.ResultsHepatic steatosis was observed in 80 subjects (23.3%: 29.6%, males; 15.0%, females). Multivariate analysis showed an association with hepatic steatosis for male gender (P=0.0212), advancing age (P=0.0241), elevated BMI (P<0.0001), elevated WHR (P=0.0024), alanine aminotransferase (P=0.0046), proinsulin (P=0.0403) and proinsulin-to-insulin ratio (P=0.0116).ConclusionsThere is an association between elevated proinsulin concentrations and hepatic steatosis.
Structural and Piezoresistive Characteristics of Amorphous Silicon Carbide Films Grown on AlN/Si Substrates
Authors: Fraga Mariana A. , Furlan Humber , Pessoa Rodrigo S. , Rasia Luiz A. 
Published: November 2012

ABSTRACTAmorphous silicon carbide (a-SiC) thin films have been grown on aluminum nitride (AlN) intermediate layers on (100) Si substrates by RF magnetron sputtering technique. Profilometry, four-point probe method, Rutherford backscattering spectroscopy (RBS) and Fourier transform infrared (FTIR) were employed to characterize the as-deposited SiC thin films. Test structures have been developed to investigate the piezoresistive properties. These structures consist of SiC thin-film resistors on AlN/Si substrates defined by reactive ion etching (RIE) with Ti/Au pads formed by lift-off process. Gauge factor (GF) and temperature coefficient of resistance (TCR) measurements have been performed and demonstrated the potential of these resistors to be used as sensing elements in devices for high temperature application.
Effects of Ce-Based Dopants on the Hydrogen Storage Material of NaAlH4
Authors: Hu Jianjiang , Witter Raiker , Ren Shuhua , Fichtner Maximilian 
Published: November 2012

ABSTRACTCerium in various chemical forms was introduced into NaAlH4 to study the hydrogen sorption properties of the resulted material. Although all the Ce precursors tested in this work resulted in a reversible hydrogen storage material, an immediate enhancement in the desorption kinetics could be achieved by a heating treatment, resulting in the in situ formation of cerium aluminide (CeAl4) in the material. While the use of CeAl4 instead of CeCl3 can increase the hydrogen capacity by bypassing the formation of the ineffective NaCl, the highest capacity of 4.9 wt% was obtained from NaAlH4 doped directly with commercial metallic cerium, which may provide a much simplified process for a possible up-scaling preparation of this hydrogen storage material.
Charge Injection Properties of Thermally-Prepared Iridium Oxide Films
Authors: Robblee Lois S. , Mangaudis Michael J. , Lasinsky Ellen D. , Kimball Angela G. , Brummer S. Barry 
Published: November 2012

ABSTRACTMultilayered Ir oxide films were prepared on Ti and Pt:Ir electrodes by the thermal decomposition of successive layers of alcohol solutions of IrCl3. The charge capacity of the thermally-formed Ir oxide films (TIROF) and the reversibility of surface oxide-related reactions were evaluated in a physiological saline solution using potential sweep and current pulse techniques. The TIROF-coated electrodes had electrochemical properties similar to an Ir electrode coated with an anodically-formed oxide. The charge injection limits before gassing ranged from 5–10 mC/cm2 for TIROF-Ti and 1–3 mC/cm2 for TIROF-Pt:Ir with biphasic, charge-balanced current pulses of 0.2 ms duration. No Pt dissolution was detected during >100 hr stimulation with TIROF-Pt:Ir electrodes. Small amounts of Ir dissolution were detected which might result from passive leaching of residual chloride complexes of Ir. The TIROF coatings remained firmly attached to the substrate metal during long term pulsing and abrasion tests. The properties of TIROF coatings, e.g., high charge capacity for reversible charge injection and good abrasion and corrosion resistance, are of great advantage for neural prosthesis applications.
MyD88 or TRAM Knockdown Regulates Interleukin (IL)‐6, IL‐8, and CXCL12 mRNA Expression in Human Gingival and Periodontal Ligament Fibroblasts
Authors: Morandini Ana Carolina , Chaves Souza Pedro Paulo , Ramos‐Junior Erivan Schnaider , Souza Costa Carlos Alberto , Santos Carlos Ferreira 
Published: November 2012

Background: In a previous report, it was shown that Toll‐like receptor (TLR) 2 knockdown modulates interleukin (IL)‐6 and IL‐8 but not the chemokine CXCL12, an important mediator with inflammatory and proangiogenic effects, in human gingival fibroblasts (HGF) and human periodontal ligament fibroblasts (HPDLF). This study investigates whether knocking down two important TLR adaptor molecules, such as myeloid differentiation protein 88 (MyD88) and TRIF‐related adaptor molecule (TRAM), could affect mRNA expression of IL‐6, IL‐8, and CXCL12 in HGF and HPDLF.Methods: After small interfering (si) RNA‐mediated silencing of MyD88 or TRAM, HGF and HPDLF were stimulated with Porphyromonas gingivalis (Pg) lipopolysaccharide (LPS) or two synthetic ligands of TLR2 (Pam2CSK4 and Pam3CSK4) for 6 hours. IL‐6, IL‐8, and CXCL12 mRNAs were evaluated by quantitative polymerase chain reaction.Results: Knockdown of MyD88 or TRAM partially impaired the IL‐8 mRNA upregulation in both fibroblast subpopulations. Similarly, IL‐6 upregulation was partially prevented by siMyD88 or siTRAM in HGF stimulated with Pg LPS, as well as in both fibroblast subtypes challenged with Pam2CSK4. Conversely, constitutive CXCL12 mRNA levels were upregulated by MyD88 or TRAM knockdown in non‐stimulated cells.Conclusions: These results suggest that TLR adaptor molecules knockdown, such as MyD88 or TRAM, can decrease IL‐6 and IL‐8 mRNA and increase CXCL12 mRNA expression in HGF and HPDLF. This can be an important step for better understanding the mechanisms that control the inflammatory cytokine and chemokine expression, which in turn contributes to periodontal pathogenesis.
Orally Administered Liposomal Lactoferrin Inhibits Inflammation‐Related Bone Breakdown Without Interrupting Orthodontic Tooth Movement
Authors: Kawazoe A. , Inubushi T. , Miyauchi M. , Ishikado A. , Tanaka E. , Tanne K. , Takata T. 
Published: November 2012

Background: Bovine lactoferrin (bLF) modulates the production of tumor necrosis factor‐alpha (TNF‐α) and inhibits alveolar bone breakdown associated with periodontitis. This study is designed to examine the effects of orally administered liposomal bLF (LbLF) on orthodontic force (OF)‐induced alveolar bone remodeling during experimental tooth movement.Methods: Two groups of male Wistar rats were treated with either LbLF or control solution in drinking water 7 days before OF application. Lipopolysaccharide (LPS) was injected into the gingival sulcus in half the rats in each group. Thus, four groups: OF, OF+LbLF, OF+LPS, and OF+LPS+LbLF were established.Results: Orally administered LbLF significantly reduced apical migration of junctional epithelium in the OF and OF+LPS groups. In OF+LPS, osteoclast number in the alveolar crestal area was increased by LPS treatment, whereas osteoclast number was significantly reduced in OF+LPS+LbLF through suppression of TNF‐α production. Osteoclastic induction in the middle part, mainly from OF application, was not affected by LbLF administration. Inhibition of tooth movement was not induced by LbLF.Conclusions: Orally administered LbLF significantly inhibits LPS‐induced alveolar bone resorption but not OF‐induced bone remodeling. LbLF could be a potent therapeutic and preventive agent to control periodontal inflammation in patients undergoing orthodontic treatment.
Timing of cranioplasty after decompressive craniectomy for ischemic or hemorrhagic stroke
Authors: Piedra Mark P. , Ragel Brian T. , Dogan Aclan , Coppa Nicholas D. , Delashaw Johnny B. 
Published: November 2012

Object
The optimal timing of cranioplasty after decompressive craniectomy for stroke is not known. Case series suggest that early cranioplasty is associated with higher rates of infection while delaying cranioplasty may be associated with higher rates of bone resorption. The authors examined whether the timing of cranioplasty after decompressive craniectomy for stroke affects postoperative complication rates.

Methods
A retrospective cohort study was undertaken to evaluate complication rates in patients undergoing cranioplasty at early (within 10 weeks of craniectomy) or late (≥ 10 weeks) stages. Multivariate logistic regression analysis was used to determine characteristics that would predict complications in patients undergoing cranioplasty after decompressive craniectomy for stroke.

Results
While the overall complication rate was higher in the early cranioplasty cohort (22% vs 16% in the late cranioplasty cohort), the difference was not statistically significant (p = 0.5541). Patients in the early cranioplasty cohort had lower rates of postoperative hematoma but higher rates of infection. Presence of a CSF shunt was the only significant predictor of complications (OR 8.96, 95% CI 1.84–43.6).

Conclusions
Complications rates for early cranioplasty (within 10 weeks of craniectomy) are similar to those encountered when cranioplasty is delayed, although the cohort size in this study was too small to state equivalence. Patients with a ventriculoperitoneal shunt are at higher risk for complications after cranioplasty.