bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hematopoietic engraftment following transplantation of bone marrow cells carrying a Philadelphia (Ph′)‐like chromosome
Authors: Graze Peter , Sparkes Robert , Como Ronald , Gale Robert P. 
Published: May 2013

AbstractA phenotypically normal donor for bone marrow transplantation was found to have a previously unreported karyotype, 46, XY, t(18q+; 22q‐), resulting in a Ph′‐like chromosome. Identification of the Ph′‐like chromosome in cultures of skin fibroblasts, phytohemaglutinin‐stimulated peripheral blood cells, and bone marrow cells from the marrow donor, but not in cell cultures from siblings or parents, indicated that it represented an acquired somatic mutation. Demonstration of the Ph′‐like chromosome in the marrow graft recipient's blood and bone marrow cells after transplantation provided a unique and definitive marker of engraftment. Hematopoiesis appeared normal in both the donor and recipient after transplantation. This study indicates that a mutation creating a Ph′‐like chromosome in hematopoietic cells need not produce hematologic abnormality. Presence of this translocation did not appear to interfere with normal hematopoietic or lymphoid differentiation and replication in the transplant setting.
Multiple myeloma terminating in lymphocytic leukemia with B‐lymphocyte membrane markers
Authors: Polliack Aaron , Naparstek Elizabeth , Eldor Amiram , Zvi Amos Ben , Bassat Hannah Ben , Zlotnick Avinoam 
Published: May 2013

AbstractThe development of lymphocytic leukemia with a rapidly fatal clinical course is reported in a patient with kappa light‐chain multiple myeloma treated with alkeran. The leukemic cells lacked the ultrastructural features of plasma cells but bore readily detectable B‐cell markers and resembled lymphocytes under the light, transmission, and scanning electron microscopes. The leukemic phase is perhaps best defined as lymphocytic and probably represents a variant of plasma cell leukemia, in which the cells showed a degree of dedifferentiation from plasma cells to B lymphocytes. The possible relation between these 2 proliferative processes is discussed and the nature of leukemias developing in cases of plasma cell myeloma is briefly reviewed.
Chronic lymphocytic leukemia‐well‐differentiated lymphocytic lymphoma with monoclonal iga: IgA production by circulating lymphocytes
Authors: Stein Richard S. , Kozin Franklin , Rothwell David 
Published: May 2013

AbstractA patient is described in whom morphologic features of well‐differentiated lymphocytic lymphoma (WDL) or chronic lymphocytic leukemia (CLL) and immunologic features of multiple myeloma were present. Studies of isolated peripheral blood lymphocytes demonstrated de novo synthesis of IgA k, the same paraprotein that was identified in the serum. Predominant IgA synthesis by peripheral lymphocytes has not been previously shown in either WDL or CLL.
Acute myelogenous leukemia subsequent to therapy for a different neoplasm: Clinical features and response to therapy
Authors: Preisler Harvey D. , Lyman Gary H. 
Published: May 2013

AbstractThe clinical characteristics of 10 patients with acute myelogenous leukemia (AML) which developed subsequently to treatment for another neoplasm are described. This disease appears to differ from “spontaneous” AML in being associated with lesser degrees of leukemic infiltration of the marrow and more frequent chromosomal aberrations. Only one of the nine patients who received chemotherapy attained remission status, and the mean and median survivals from the initiation of chemotherapy were 2.7 months and one month respectively. Nine of the 10 patients died as a result of infection. The refractoriness of this form of AML to chemotherapy was borne out by a review of the literature, which revealed only two remissions in 32 treated patients. The implications for the management of this disease are discussed.
Chemotaxis radioassays: A simplified, quantitative method using technetium‐99m radiocolloid labeled granulocytes
Authors: English Denis , McPherson T. A. 
Published: May 2013

AbstractSelective labeling of phagocytes in human blood can be achieved by incubation of unseparated, heparinized blood with 99mTc‐sulfur colloid. We have developed a method for quantitation of granulocyte Chemotaxis using granulocytes labeled by this technique. The method was found to be more accurate and less tedious to perform than the conventional Boyden chamber Chemotaxis assay. The assay was also less time‐consuming and as quantitative as a previously described granulocyte Chemotaxis radioassay in which 51Cr‐labeled leukocytes were used. The simplicity and reliability of this technique and the ready availability of 99mTc‐sulfur colloid may provide one standardized method for granulocyte Chemotaxis quantitation in clinical and experimental medicine.
Cationic starch for microalgae and total phosphorus removal from wastewater
Authors: Anthony Renil , Sims Ronald 
Published: May 2013

ABSTRACTThe city of Logan, Utah treats wastewater in 460 acres of facultative lagoons. Naturally growing algae in these lagoons uptake nutrients and remediate the wastewater, but require coagulation and flocculation for separation from wastewater. Cationic corn starch and cationic potato starch with a degree of substitution 1.34 and 0.82, respectively, were synthesized using 3‐methacryloyl amino propyl trimethyl ammonium chloride and tested on Scenedesmus obliquus and lagoon wastewater. The zeta potential of the cationic starches tested over a pH range 5.0–10.0 showed an average of +16.0 and +15.1 mV for cationic corn starch and cationic potato starch, respectively. Total suspended solids removals of 90 and 85% were achieved with cationic corn starch and cationic potato starch, respectively, when tested with S. obliquus. Tests with wastewater showed total suspended solids removals of 80 and 60% for cationic corn starch and cationic potato starch, respectively. Total phosphorus removal values from wastewater were approximately 33, 29, and 42% for cationic corn starch, cationic potato starch, and alum, respectively, for a coagulant/algae ratio of 1.4 (wt/wt). These results indicate that cationic starch has the potential to replace alum for algae harvesting and wastewater treatment making it a suitable alternative to inorganic coagulants and flocculants. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2572–2578, 2013
Similarity and stability analysis of the two partitioning type clustering algorithms
Authors: Can Fazli , Ozkarahan Esen A. 
Published: May 2013

AbstractThe similarity and stability analysis of the two partitioning type clustering algorithms, which appeared in this journal recently, are presented with respect to two different metrics. This article also includes other aspects of the algorithms, such as the number and size of the clusters generated, the similarity between the members of a cluster and the corresponding centroid, and other relevant details. In the experiments, a document collection consisting of the titles, abstracts, and keywords of 167 ACM TODS publications was used.
Homocysteine causes disruptions in spinal cord morphology and changes the expression of Pax 1/9 and Sox 9 gene products in the axial mesenchyme
Authors: Kobus Karoline , Ammar Dib , Nazari Evelise Maria , Rauh Müller Yara Maria 
Published: May 2013

BACKROUNDNeural tube defects (NTD) involve disruptions in the axial mesenchyme, and are related to an imbalance between folic acid (FA) and homocysteine (Hcy). This study evaluated the effects of FA/Hcy imbalance on cell proliferation and expression of the Pax 1/9 and Sox 9 gene products in the axial mesenchyme of chickens.METHODSEmbryos were incubated (38°C) and pretreated at 24 h and treated at 46 h of incubation. The experimental groups were: FA‐pretreated with saline and treated with 0.5 μg FA/saline; Hcy‐pretreated with 50 μl saline and treated with 20 μmol D,L‐Hcy/50 μl saline; FA+Hcy‐pretreated with 0.5 μg FA/50 μl saline and treated with 20 μmol D,L‐Hcy/50 μl saline; and the control embryos were pretreated and treated with saline. Embryos were analyzed at E4 and E6. Immunohistochemistry was performed to identify proliferating cells and the expression of the gene products of Pax 1/9 and Sox 9. Total RNA of the E4 embryos was extracted and a RT‐qPCR assay was performed to quantify Pax 1/9 mRNA expression.RESULTSHcy treatment caused spinal NTD and abnormalities in axial mesenchyme development, affecting the distribution of sclerotomal cells and chondrification. Hcy also reduced cell proliferation and changed the expression of Pax 1/9 and Sox 9 in the mesenchyme.CONCLUSIONSOur data clarified the relationship between spinal NTD genesis and disruptions of Pax 1/9 and Sox 9 gene products in the axial mesenchyme caused by the FA/Hcy imbalance. Birth Defects Research (Part A) 97:386–397, 2013. © 2013 Wiley Periodicals, Inc.
Prenatal exposure to nitrosatable drugs, vitamin C, and risk of selected birth defects
Authors: Shinde Mayura U. , Vuong Ann M. , Brender Jean D. , Werler Martha M. , Kelley Katherine E. , Huber John C. , Sharkey Joseph R. , Zheng Qi , Suarez Lucina , Langlois Peter H. , Canfield Mark A. , Romitti Paul A. , Malik Sadia ,   The National Birth Defects Prevention Study
Published: May 2013

BACKGROUNDNitrosatable drugs, such as secondary or tertiary amines and amides react with nitrite in an acidic environment to form N‐nitroso compounds, teratogens in animal models. Vitamin C is a known nitrosation inhibitor.METHODSUsing data from the National Birth Defects Prevention Study, we assessed nitrosatable drug exposure and vitamin C intake during the first trimester among 11,606 case‐mothers of infants with oral clefts, limb deficiencies (LDs), or congenital heart defects and 6807 control‐mothers of infants without major birth defects during 1997–2005. Daily intake of vitamin C was estimated from maternal interviews that elicited information about supplement use and dietary intake.RESULTSWith no reported use of nitrosatable drugs as the referent group, a lower odds ratio (OR) was observed for transverse LDs among births to mothers exposed to secondary amine drugs and daily vitamin C supplementation (adjusted odds ratio [aOR] 1.2, 95% confidence interval [CI] 0.83–1.8) compared with women taking these drugs and no supplementation (aOR 2.7, 95% CI 1.5–4.6). The OR for longitudinal LDs associated with secondary amine exposure was lower with daily dietary vitamin C intake ≥85 mg (aOR 1.2, 95% CI 0.68–2.0) compared with <85 mg (aOR 1.9, 95% CI 1.2–3.1). Daily vitamin C supplementation in combination with higher dietary vitamin C intake reduced associations between nitrosatable drug exposures and limb deficiencies and atrial septal defects not otherwise specified.CONCLUSIONPrenatal dietary and vitamin C supplement intake may diminish the association between nitrosatable drug exposure during pregnancy and selected birth defects. Birth Defects Research (Part A) 97:515‐531, 2013. © 2013 Wiley Periodicals, Inc.
Toward a universal treatment for cancer: cell inflation assisted chemotherapy
Authors: Corcos Daniel 
Published: May 2013

AbstractCancers show considerable genetic and functional heterogeneity, preventing the development of a universal anticancer drug. Here, I argue that it is nevertheless possible to elaborate a therapeutic strategy that can be used in almost every cancer, exploiting the negative feedback effect of normal cells on the proliferation of their precursors. This method, termed cell inflation assisted chemotherapy, is aimed at blocking normal cell division prior to high‐dose antimitotic chemotherapy. Evidence for a negative feedback effect on granulocyte production suggests that it is possible to prevent neutropenia by transfusion of autologous granulocytes. In a first step, this protocol will be devised to protect neutrophils and to prevent granulopenia in patients treated with intensive chemotherapy. In its simplest form, it will consist of a leukapheresis–storage–reinjection sequence just prior to drug administration. Then, if the proof of concept is established, a more systematic use of intensive cell cycle‐specific chemotherapy, together with protection of other lineages through temporary mitotic blockade might be a treatment applicable for most cancers.
Reduction in serum clusterin is a potential therapeutic biomarker in patients with castration‐resistant prostate cancer treated with custirsen
Authors: Blumenstein Brent , Saad Fred , Hotte Sebastien , Chi Kim N. , Eigl Bernhard , Gleave Martin , Jacobs Cindy 
Published: May 2013

AbstractElevated levels of clusterin (CLU), a stress‐induced and secreted cytoprotective chaperone, are associated with advanced tumor stage, metastasis, treatment resistance, and adverse outcome in several cancers. Custirsen, a second‐generation antisense oligonucleotide, inhibits CLU production in tumor cells and reduces serum CLU levels. A Phase 2 study evaluated custirsen in combination with second‐line chemotherapy in men with metastatic castration‐resistant prostate cancer (mCRPC) who had progressed while on or within 6 months of first‐line docetaxel‐based chemotherapy. Exploratory analyses evaluated serum CLU levels during custirsen treatment and correlative clinical effects on prostate‐specific antigen (PSA) response, overall survival, and any relationship between serum CLU and PSA. Men with mCRPC were treated with mitoxantrone/prednisone/custirsen (MPC, n = 22) or docetaxel retreatment/prednisone/custirsen (DPC plus DPC‐Assigned, n = 45) in an open‐label, multicenter study. Subject‐specific profiles of PSA and serum CLU levels during treatment were characterized using statistical modeling to compute subject‐specific summary measures; these measures were analyzed for relationship to survival using proportional hazard regression. Estimated individual serum CLU response profiles were scored as below or at/above the median level for the population through 100 days postrandomization. Median survival was longer for subjects scoring below the median serum CLU level compared with subjects at/above the median level, respectively (MPC: 15.1 months vs. 6.2 months; DPC‐Pooled: 17.0 months vs. 12.1 months). Lowered serum CLU levels during custirsen treatment when in combination with either chemotherapy regimen were predictive of longer survival in mCRPC. These results support further evaluation of serum CLU as a therapeutic biomarker.
Racial differences in time from prostate cancer diagnosis to treatment initiation
Authors: Stokes William A. , Hendrix Laura H. , Royce Trevor J. , Allen Ian M. , Godley Paul A. , Wang Andrew Z. , Chen Ronald C. 
Published: May 2013

BACKGROUNDTimely delivery of care has been identified by the Institute of Medicine as an indicator for quality health care, and treatment delay is a potentially modifiable obstacle that can contribute to the disparities among African American (AA) and Caucasian patients in prostate cancer recurrence and mortality. Using the Surveillance, Epidemiologic and End Results (SEER)‐Medicare linked database, we compared time from diagnosis to treatment in AA and Caucasian prostate cancer patients.METHODSA total of 2506 AA and 21,454 Caucasian patients diagnosed with localized prostate cancer from 2004 through 2007 and treated within 12 months were included. Linear regression was used to assess potential differences in time to treatment between AA and Caucasian patients, after adjusting for sociodemographic and clinical covariates.RESULTSTime from diagnosis to definitive (prostatectomy and radiation) treatment was longer for AA patients in all risk groups, and most pronounced in high‐risk cancer (96 versus 105 days, P < .001). On multivariate analysis, racial differences to any and definitive treatment persisted (β = 7.3 and 7.6, respectively, for AA patients). Delay to definitive treatment was longer in high‐risk (versus low‐risk) disease and in more recent years.CONCLUSIONSAA patients with prostate cancer experienced longer time from diagnosis to treatment than Caucasian patients with prostate cancer. AA patients appear to experience disparities across all aspects of this disease process, and together these factors in receipt of care plausibly contribute to the observed differences in rates of recurrence and mortality among AA and Caucasian patients with prostate cancer. Cancer 2013;119:2486‐2493. © 2013 American Cancer Society.
Chip electrophoresis of gelatin‐based nanoparticles
Authors: Weiss Victor U. , Lehner Angela , Grombe Ringo , Marchetti‐Deschmann Martina , Allmaier Günter 
Published: May 2013

Recently, biodegradable nanoparticles received increasing attention for pharmaceutical applications as well as applications in the food industry. With the current investigation we demonstrate chip electrophoresis of fluorescently (FL) labeled gelatin nanoparticles (gelatin NPs) on a commercially available instrument. FL labeling included a step for the removal of low molecular mass material (especially excess dye molecules). Nevertheless, for the investigated gelatin NP preparation two analyte peaks, one very homogeneous with an electrophoretic net mobility of μ = −24.6 ± 0.3 × 10−9 m2/Vs at the peak apex (n = 17) and another more heterogeneous peak with μ between approximately −27.2 ± 0.2 × 10−9 m2/Vs and −36.6 ± 0.2 × 10−9 m2/Vs at the peak beginning and end point (n = 11, respectively) were recorded. Filtration allowed enrichment of particles in the size range of approximately 35 nm (pore size employed for concentration of gelatin NPs) to 200 nm (pore size employed during FL labeling). This corresponded to the very homogeneous peak linking it to gelatin NPs, whereas the more heterogeneous peak probably corresponds to gelatin not cross‐linked to such a high degree (NP building blocks). Several further gelatin NP preparations were analyzed according to the same protocol yielding peaks with electrophoretic net mobilities between −23.3 ± 0.3 × 10−9 m2/Vs and −28.9 ± 0.2 × 10−9 m2/Vs at peak apexes (n = 15 and 6). Chip electrophoresis allows analyte separation in less than two minutes (including electrophoretic sample injection). Together with the high sensitivity of the FL detection – the LOD as derived for the first main peak of the applied dye from the threefold standard deviation of the background noise values 80 pM for determined separation conditions – this leads to a very promising high throughput separation technique especially for the analysis of bionanoparticles. For gelatin NP preparations, chip electrophoresis allows for example the comparison of preparation batches concerning the amount of NPs and gelatin building blocks as well as the indirect assessment of the degree of gelatin cross‐linking (from obtained FL signals).
A new approach to the studies of submicron particle suspensions based on the effect of pressure in capillary zone electrophoresis
Authors: Vanifatova Nataliya , Rudnev Alexander , Spivakov Boris 
Published: May 2013

A new approach based on the effect of pressure in CZE is suggested for acceleration of particle migration in electrophoretic runs resulting in reduction of the analysis time. It provides conditions for studying fast processes in suspensions. The effect of pressure on the migration of silica spheres with average diameters of 100, 150, and 390 nm was studied by CZE at an applied voltage of 25 kV. The particle hydrodynamic behavior was also investigated under the same capillary dimensions and BGE composition. The total particle mobility (excluding the average flow rate) was found to increase with increasing the pressure applied and particle size. The particle migration mechanism explaining the effect of pressure on particle velocity was shown to be almost the same as in wide‐bore hydrodynamic chromatography. It is based on changing radial distribution of particle concentration along the capillary cross section depending on particle diffusivity. On the basis of this mechanism appearance of a zone of negatively charged particles in electropherograms ahead of the marker peak can be explained.
Extreme densities in Titan's ionosphere during the T85 magnetosheath encounter
Authors: T. Edberg N. J. , Andrews D. J. , Shebanits O. , Ågren K. , Wahlund J.‐E. , Opgenoorth H. J. , Roussos E. , Garnier P. , Cravens T. E. , Badman S. V. , Modolo R. , Bertucci C. , Dougherty M. K. 
Published: May 2013

AbstractWe present Cassini Langmuir probe measurements of the highest electron number densities ever reported from the ionosphere of Titan. The measured density reached 4310 cm−3 during the T85 Titan flyby. This is at least 500 cm−3 higher than ever observed before and at least 50% above the average density for similar solar zenith angles. The peak of the ionospheric density is not reached on this flyby, making the maximum measured density a lower limit. During this flyby, we also report that an impacting coronal mass ejection (CME) leaves Titan in the magnetosheath of Saturn, where it is exposed to shocked solar wind plasma for at least 2 h 45 min. We suggest that the solar wind plasma in the magnetosheath during the CME conditions significantly modifies Titan's ionosphere by an addition of particle impact ionization by precipitating protons.
Hemodynamic assessment of indomethacin‐induced fetal heart failure in high‐output state
Authors: Tongsong Theera , Luewan Suchaya , Srisupundit Kasemsri , Jatavan Thanate 
Published: May 2013

ABSTRACTHemodynamic study was performed on a 32‐year‐old woman presenting at 27 weeks' gestation. Ultrasound revealed a single normal fetus with mild cardiomegaly, polyhydramnios, and placental chorioangioma. Doppler study showed increased middle cerebral artery peak systolic velocity, normal Tei index, and forward flow of “a” wave with normal preload in the ductus venosus. Twelve hours after initiation of indomethacin for tocolysis, frank hydrops fetalis developed rapidly. The right ventricle showed poor contractility and performance. Markedly increased preload in ductus venosus with reversed “a” wave and pulsations in the umbilical vein were demonstrated. This study suggests that indomepacin treatment in fetal high‐cardiac output state should be used with extreme caution. © 2013 Wiley Periodicals, Inc. J Clin Ultrasound 41:438–440, 2013
Chiral pH‐Responsive Amphiphilic Polymer Co‐networks: Preparation, Chiral Recognition, and Release Abilities
Authors: Shi Lu , Xie Peng , Li Zhimin , Wu Youping , Deng Jianping 
Published: May 2013

AbstractNovel amphiphilic polymer co‐networks (APCNs), poly(N‐acryloyl‐L‐alanine)‐l‐polydimethylsiloxane (denoted as PNAA‐l‐PDMS), are prepared, and exhibit remarkable pH‐responsiveness, chiral‐recognition, and enantioselective‐release abilities. The APCNs are prepared by free‐radical copolymerization starting from N‐acryloyl‐L‐alanine (NAA) and methacrylate‐terminated poly(dimethylsiloxane) (M‐PDMS) as co‐(macro)monomers. The APCNs show pronounced pH‐sensitivity, evidenced by a reversible swelling–deswelling transition upon a cyclicly altering pH. The chiral co‐networks are applied for enantioselective recognition and release. A maximum adsorption is achieved towards D‐proline (61%), whereas for L‐proline, it is only 10%. More interestingly, the release for the L‐proline is 90%, whereas for the D‐proline, only 70% is released.magnified image