bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell-Free DNA Next-Generation Sequencing in Pancreatobiliary Carcinomas
Authors: Zill Oliver A. , Greene Claire , Sebisanovic Dragan , Siew Lai Mun , Leng Jim , Vu Mary , Hendifar Andrew E. , Wang Zhen , Atreya Chloe E. , Kelley Robin K. , Van Loon Katherine , Ko Andrew H. , Tempero Margaret A. , Bivona Trever G. , Munster Pamela N. , Talasaz AmirAli , Collisson Eric A. 
Published: June 2015

Abstract
Patients with pancreatic and biliary carcinomas lack personalized treatment options, in part because biopsies are often inadequate for molecular characterization. Cell-free DNA (cfDNA) sequencing may enable a precision oncology approach in this setting. We attempted to prospectively analyze 54 genes in tumor and cfDNA for 26 patients. Tumor sequencing failed in 9 patients (35%). In the remaining 17, 90.3% (95% confidence interval, 73.1%–97.5%) of mutations detected in tumor biopsies were also detected in cfDNA. The diagnostic accuracy of cfDNA sequencing was 97.7%, with 92.3% average sensitivity and 100% specificity across five informative genes. Changes in cfDNA correlated well with tumor marker dynamics in serial sampling (r = 0.93). We demonstrate that cfDNA sequencing is feasible, accurate, and sensitive in identifying tumor-derived mutations without prior knowledge of tumor genotype or the abundance of circulating tumor DNA. cfDNA sequencing should be considered in pancreatobiliary cancer trials where tissue sampling is unsafe, infeasible, or otherwise unsuccessful.
Significance: Precision medicine efforts in biliary and pancreatic cancers have been frustrated by difficulties in obtaining adequate tumor tissue for next-generation sequencing. cfDNA sequencing reliably and accurately detects tumor-derived mutations, paving the way for precision oncology approaches in these deadly diseases. Cancer Discov; 5(10); 1040–8. ©2015 AACR.
This article is highlighted in the In This Issue feature, p. 1005
Recurrence of Atrial Tachyarrhythmia During the Second Month of the Blanking Period Is Associated With More Extensive Pulmonary Vein Reconnection at Repeat Electrophysiology Study
Authors: Das Moloy , Wynn Gareth J. , Morgan Maureen , Lodge Ben , Waktare Johan E.P. , Todd Derick M. , Hall Mark C.S. , Snowdon Richard L. , Modi Simon , Gupta Dhiraj 
Published: June 2015

Background—
Current guidelines recommend a 3-month blanking period after pulmonary vein isolation (PVI) as early recurrence of atrial tachyarrhythmia (ERAT) may be due to transient proarrhythmic factors. However, studies have suggested that these factors resolve by 1 month. PV reconnection (PVrc) is strongly associated with postblanking AT recurrence in paroxysmal atrial fibrillation. We hypothesized that ERAT occurring beyond 4 weeks after PVI is associated with PVrc at repeat electrophysiology study.

Methods and Results—

Forty patients with paroxysmal atrial fibrillation underwent mandatory repeat electrophysiology study 2 months after PVI, regardless of symptoms, to document the number of reconnected PVs. Antiarrhythmic drugs, including β-blockers, were discontinued 4 weeks after PVI. Patients were instructed to record a 30-second ECG everyday between the 2 procedures using a portable monitor, with additional recordings for symptoms. ERAT was defined as ≥30 seconds of AT. Patients recorded a total of 3293 ECGs. Four (10%) patients had ERAT in the first 4 weeks (M1) only, 2 (5%) in month 2 (M2) only, and 11 (28%) in both. PVrc of 1 PV was identified in 12 (30%) patients and of >1 PV in 13 (32%) patients. ERAT in M2 was associated with PVrc, whereas M1 was not (11/13 [85%] versus 0/4 [0%];
P
=0.006). M2 ERAT was strongly associated with PVrc of >1 PV (10/13 [77%] versus 3/27 [11%] without M2 ERAT;
P
<0.0001).

Conclusions—
ERAT occurring beyond 4 weeks after PVI is associated with PVrc and particularly of PVrc of >1 PV. ERAT confined to M1 is unrelated to underlying PVrc. The relationship between ERAT beyond 4 weeks after PVI and postblanking AT recurrence merits further investigation.
Subseasonal Variability of Precipitation in China during Boreal Winter
Authors: Yao Yonghong , Lin Hai , Wu Qigang 
Published: June 2015

Abstract
Using pentad data of the Northern Hemisphere extended winter (November–March) from 1979 to 2012 derived from the daily rainfall of the National Meteorological Information Center of China, subseasonal variability of precipitation in China is analyzed. The two dominant modes of subseasonal variability are identified with an empirical orthogonal function (EOF) analysis. The first EOF mode (EOF1) is characterized by a monopole in South China, whereas the second EOF mode (EOF2) has a meridional dipole structure with opposite precipitation anomalies over the Yangtze River basin and the coastal area of South China. These two modes tend to have a phase shift to each other in both space and time, indicating that part of their variability represents a southward-propagating pattern.
The subseasonal variability is decomposed into two components: one related to the Madden–Julian oscillation (MJO) and the other independent of MJO. It is found that the MJO contributes to about 10% of the precipitation variability in South China. EOF1 is associated with MJO phase 3, corresponding to enhanced equatorial convection in the Indian Ocean and depressed convection in the western Pacific, while EOF2 is related to MJO phase 5 when the enhanced tropical convection moves to the Maritime Continent region. Subseasonal precipitation variability in China that is independent of the MJO is especially affected by processes including tropical convection variability and the “cold surge” phenomenon or the development of a Siberian high and cold-air outbreak in East Asia associated with a wave train from the North Atlantic.
A Probabilistic Wavelet–Support Vector Regression Model for Streamflow Forecasting with Rainfall and Climate Information Input*
Authors: Liu Zhiyong , Zhou Ping , Zhang Yinqin 
Published: June 2015

Abstract
It is essential to explore reliable streamflow forecasting techniques for water resources management. In this study, a Bayesian wavelet–support vector regression model (BWS model) is developed for one- and multistep-ahead streamflow forecasting using local meteohydrological observations and climate indices including El Niño–Southern Oscillation (ENSO) and the Indian Ocean dipole (IOD) as potential predictors. To accomplish this, a two-step strategy is applied. In the first step, the discrete wavelet transform is coupled with a support vector regression model for streamflow prediction. The three key factors of mother wavelets, decomposition levels, and edge effects are considered in the wavelet decomposition phase when using the hybrid wavelet–support vector regression model (WS model). Different combinations of these factors form a variety of WS models with corresponding forecasts. The second step combines multiple candidate WS models with “good” performance via Bayesian model averaging. This integrates the predictive strengths of different candidate WS models, giving a realistic assessment of the predictive uncertainty. The new ensemble model is used to forecast daily and monthly streamflows at two sites in Dongjiang basin, southern China. The results show that the proposed BWS model consistently generates more reliable predictions for daily (lead times of 1–7 days) and monthly (lead times of 1–3 months) forecasts as compared with the best single-member WS models and the adaptive neuro-fuzzy inference system (ANFIS). Furthermore, the proposed BWS model provides detailed information about the predictive uncertainty.
Case Study of Moisture and Heat Budgets within Atmospheric Rivers
Authors: Luo Qianwen , Tung Wen-wen 
Published: June 2015

Abstract
This work studies moisture and heat budgets within two atmospheric rivers (ARs) that made landfall on the west coast of North America during January 2009. Three-dimensional kinematic and thermodynamic fields were constructed using ECMWF Year of Tropical Convection data and global gridded precipitation datasets. Differences between the two ARs are observed, even though both had embedded precipitating convective organizations of the same spatial scale. AR1 extended from 20° to 50°N in an almost west–east orientation. It had excessive warm and moist near-surface conditions. Its precipitating systems were mainly distributed on the southwest and northeast sides of the AR, and tended to exhibit stratiform-type vertical heat and moisture transports. In contrast, AR2 spanned latitudes between 20° and 60°N in a north–south orientation. It was narrower and shorter than AR1, and was mostly covered by pronounced precipitating systems, dominated by a deep convection type of heating throughout the troposphere. In association with these distinctions, the atmosphere over the northeastern Pacific on average experienced episodic cooling and drying despite the occurrence of AR1, yet underwent heating and drying during AR2, when latent heating was strong. Downward sensible heat flux and weak upward surface latent heat flux were observed particularly in AR1. In addition, cloud radiative forcing (CRF) was very weak in AR1, whereas it was strongly negative in AR2. In short, it is found that the oceanic convection in ARs both impacts the moisture transport of ARs, as well as modifies the heat balance in the midlatitudes through latent heat release, convective heat transport, surface heat fluxes, and CRF.
Occupation, Family, and Inheritance in Fourteenth-Century Barcelona: A Socio-Economic Profile of One of Europe’s Earliest Investing Publics
Authors: Fynn-Paul Jeff 
Published: June 2015

This article aims to provide a snapshot of the Barcelona urban elite during a crucial phase in the city’s transition to early modernity – the decades after the Black Death. Between 1356 and 1375, the Crown of Aragon, of which Barcelona was the principal city, was locked in a doubtful struggle with Castile over Aragon’s very independence. During the war, the financial officers of King Pere III of Catalonia-Aragon (1336–87) perfected a unique public debt system, and a cornerstone of this system was a debt instrument known as a violari. By paying high interest rates of over 14 percent per annum, from government tax revenues, the violari became an attractive investment opportunity for burghers, urban clerics, and some nobles, both male and female. Certain unusual characteristics of the violari make it a useful way to study interpersonal connections amongst elites. First, purchasers had to name a designated heir in the event of their own demise. Secondly, many purchasers bought violaris as a means of providing income to family members and other trusted relations. By studying the violari-buying elite from a combination of economic, social, and political angles, several interesting observations can be made regarding the role played by urban elites in the late medieval and early modern state-building process. The obvious strength of public support for the violari goes a long way towards explaining the strength of the Catalan-Aragonese fiscal system in the later fourteenth century, and indeed, towards explaining the Crown’s territorial expansion in the western Mediterranean, which continued through the fifteenth century. The voluntary nature of the purchases suggests that Catalonia-Aragon might be seen as one of the first territorial states to undergo a ‘financial revolution’ – seen by economic historians as a hallmark of modern state-building. At the local level, the wealth of different occupational groups can be estimated, and their political ambitions therefore thrown into greater relief. The near absence of noble investors and the presence of several urban clerics tell us much about the relations between the three ‘estates’ at this period in Barcelona’s history. The presence of many women, both widows and wives, as both purchasers and beneficiaries, paints a surprisingly egalitarian picture of female economic agency; furthermore, it appears as though nuclear family structures and property strategies predominated over ‘clan’ or ‘patrilineal’ structures commonly seen in Italian historiography. Distinct patterns of wealth allocation are also discernible amongst male and female investors.
Bioabsorbable Versus Titanium Screws in Anterior Cruciate Ligament Reconstruction Using Hamstring Autograft
Authors: Arama Yuval , Salmon Lucy J. , Sri-Ram Kesavan , Linklater James , Roe Justin P. , Pinczewski Leo A. 
Published: June 2015

Background: Longer-term results of bioabsorbable screws for anterior cruciate ligament reconstruction (ACLR) have been reported, but results are specific to the exact screw material and design. Titanium and poly-L-lactic acid with hydroxyapatite (PLLA-HA) screw outcomes have been compared only to 2 years. Purpose/Hypothesis: The purpose of this study was to compare the clinical and radiologic outcomes of the PLLA-HA screw versus titanium screw for hamstring tendon ACLR over a 5-year follow-up period. The hypothesis was that there are no differences in clinical scores or tunnel widening between the PLLA-HA and the titanium screws and that the PLLA-HA screw as seen on magnetic resonance imaging (MRI) should show high-grade resorption and ossification response over 5 years. Study Design: Randomized controlled trial; Level of evidence, 1. Methods: A total of 40 patients undergoing ACLR were randomized to receive either a PLLA-HA screw (PLLA-HA group) or a titanium screw (titanium group) for ACL fixation. Blinded evaluation was performed at 2 and 5 years with the International Knee Documentation Committee and Lysholm knee score, KT-1000 arthrometer, single-legged hop test, and MRI to evaluate tunnel and screw volumes, periscrew ossification, graft integration, and cyst formation. Results: There was no difference in any clinical outcome measure at 2- or 5-year follow-up between the 2 groups. At 2 years, the femoral tunnel in the PLLA-HA group was smaller than that in the titanium group ( P = .02); at 5 years, there was no difference. At 2 years, the femoral PLLA-HA screw was a mean 76% of its original volume, and by 5 years, it was 36%. At 2 years, the tibial PLLA-HA screw mean volume was 68% of its original volume, and by 5 years, it was 46%. At 5 years in the PLLA-HA group, 88% of femoral tunnels and 56% of tibial tunnels demonstrated a significant ossification response. There was no increase in cyst formation in the PLLA-HA group and no screw breakages. Conclusion: There were equivalent clinical results between the PLLA-HA and titanium groups at 2- and 5-year follow-ups. The PLLA-HA screw was not associated with increased tunnel widening or cyst formation when compared with the titanium screw. The PLLA-HA screw demonstrated progressive screw resorption and gradual but incomplete ossification over 5 years.
Comparison of a Household Consumption and Expenditures Survey with Nationally Representative Food Frequency Questionnaire and 24-hour Dietary Recall Data for Assessing Consumption of Fortifiable Foods by Women and Young Children in Cameroon
Authors: Engle-Stone Reina , Brown Kenneth H. 
Published: June 2015

Background: Household Consumption and Expenditures Surveys (HCES) are potential sources of data on dietary patterns for planning fortification programs, but they rarely have been compared with individual-level dietary assessment methods. Objective: To compare apparent consumption of fortifiable foods estimated from the Third Cameroon Household Survey (ECAM3) with the results of a national dietary survey using food frequency questionnaire (FFQ) and 24-hour recall (24HR) methods among women aged 15 to 49 years and children aged 12 to 59 months. Methods: We estimated coverage and frequency of consumption in the previous week (ECAM3 and FFQ) and coverage and amount consumed on the previous day (ECAM3 and 24HR) of refined vegetable oil, wheat flour, sugar, and bouillon cubes. Results: Coverage in the past week as measured by the ECAM3 and FFQ, respectively, was 64% vs. 54% for oil, 60% vs. 92% for flour, 69% vs. 78% for sugar, and 85% vs. 96% for bouillon cubes. The different methods identified similar patterns of coverage among subgroups for oil, but patterns for other foods were variable. Frequency of consumption and previous-day coverage were lower with the ECAM3 than with the FFQ and 24HR, likely reflecting infrequent acquisition relative to intake. For women, the mean amounts consumed on the previous day (among consumers) were 43 vs. 29 g of oil, 71 vs. 83 g of flour, 42 vs. 32 g of sugar, and 5.0 vs. 2.4 g of bouillon cubes (ECAM and 24HR, respectively). Conclusions: HCES provide useful information on patterns of food access but inadequate information on individual consumption amounts to estimate appropriate food fortification levels.
Measuring the Food Access Dimension of Food Security
Authors: Leroy Jef L. , Ruel Marie , Frongillo Edward A. , Harris Jody , Ballard Terri J. 
Published: June 2015

Background: With food security now a top priority for many governments and for the global development community, there is heightened awareness of the need to improve our understanding and measurement of food security. Objective: To bring clarity in the assessment of the food access dimension of food security at the household and individual level. Methods: For the most commonly used indicators, we reviewed their original purpose and construction, at what levels (household or individual) they were designed to be used, what components (quality, quantity, safety, and cultural acceptability) they were intended to reflect, and whether or not they have been tested for validity and comparability across contexts. Results: We identified nine indicators and grouped them in three broad categories: experience-based, coping strategies, and dietary diversity. The indicators only capture the quantity and quality components of food access; none of the indicators capture information on safety or cultural acceptability of food access. Household Dietary Diversity (HDDS) and Food Consumption Score (FCS) are often considered indicators of both quantity and quality, but they have not been validated for the latter. Conclusions: We recommend the use of experience-based indicators, HDDS, or FCS to assess household access to energy; experience-based indicators to assess household access to diet quality (defined qualitatively as not having to adopt practices that favor acquiring cheaper, less appealing, and less micronutrient-dense foods); and individual dietary diversity scores for women or children to assess individual access to diet quality, defined as micronutrient adequacy.
Food for Survival
Authors: Niragira Sanctus , D’Haese Marijke , D’Haese Luc , Ndimubandi Jean , Desiere Sam , Buysse Jeroen 
Published: June 2015

Background: Burundi is one of the world’s poorest countries, coming last in the Global Food Index (2013). Yet, a large majority of its population depends on agriculture. Most smallholder families do not produce enough to support their own families. Objective: To estimate the optimal crop mix and resources needed to provide the family with food containing sufficient energy, fat, and protein. Methods: This study uses mathematical programming to obtain the optimal crop mix that could maximize output given the constraints on production factor endowments and the need to feed the household. The model is calibrated with household-level data collected in 2010 in Ngozi Province in northern Burundi. Four models are developed, each representing a different farm type. The typology is based on 2007 data. Model predictions are compared with data collected during a revisit of the area in 2012. Results: By producing a smaller number of crops and concentrating on those in which they have a comparative advantage, and trading produce and input with other farms, large and medium-sized farms can improve their productivity and hire extra workers to supplement family labor. Predictions of crops to be planted coincided to a high degree with those that farmers planted 2 years after our survey on newly acquired plots. Conclusions: Despite land scarcity, it is still possible for households that own land to find optimal crop combinations that can meet their minimal food security requirements while generating a certain level of income. Nearly landless households would benefit from the increased off-farm employment opportunities. With only 0.05 ha of land per capita, the annotation Nearly Landless is used to highlight the limited access to land observed in this farm category.
Design High-Efficiency III–V Nanowire/Si Two-Junction Solar Cell
Authors: Wang Y , Zhang Y , Zhang D , He S , Li X 
Published: June 2015

Abstract
In this paper, we report the electrical simulation results of a proposed GaInP nanowire (NW)/Si two-junction solar cell. The NW physical dimensions are determined for optimized solar energy absorption and current matching between each subcell. Two key factors (minority carrier lifetime, surface recombination velocity) affecting power conversion efficiency (PCE) of the solar cell are highlighted, and a practical guideline to design high-efficiency two-junction solar cell is thus provided. Considering the practical surface and bulk defects in GaInP semiconductor, a promising PCE of 27.5 % can be obtained. The results depict the usefulness of integrating NWs to construct high-efficiency multi-junction III–V solar cells.
Mechanisms Underlying Clinical Efficacy of Angiotensin II Type 2 Receptor (AT2R) Antagonist EMA401 in Neuropathic Pain: Clinical Tissue and in Vitro Studies
Authors: Anand Uma , Yiangou Yiangos , Sinisi Marco , Fox Michael , MacQuillan Anthony , Quick Tom , Korchev Yuri E , Bountra Chas , McCarthy Tom , Anand Praveen 
Published: June 2015

Background The clinical efficacy of the Angiotensin II (AngII) receptor AT2R antagonist EMA401, a novel peripherallyrestricted analgesic, was reported recently in post-herpetic neuralgia. While previous studies have shown that AT2R is expressed by nociceptors in human DRG (hDRG), and that EMA401 inhibits capsaicin responses in cultured hDRG neurons, the expression and levels of its endogenous ligands AngII and AngIII in clinical neuropathic pain tissues, and their signalling pathways, require investigation. We have immunostained AngII, AT2R and the capsaicin receptor TRPV1 in control post-mortem and avulsion injured hDRG, control and injured human nerves, and in cultured hDRG neurons. AngII, AngIII, and Ang-(1-7) levels were quantified by ELISA. The in vitro effects of AngII, AT2R agonist C21, and Nerve growth factor (NGF) were measured on neurite lengths; AngII, NGF and EMA401 effects on expression of p38 and p42/44 MAPK were measured using quantitative immunofluorescence, and on capsaicin responses using calcium imaging. Results AngII immunostaining was observed in approximately 75% of small/medium diameter neurons in control (n = 5) and avulsion injured (n = 8) hDRG, but not large neurons i.e. similar to TRPV1. AngII was co-localised with AT2R and TRPV1 in hDRG and in vitro. AngII staining by image analysis showed no significant difference between control (n = 12) and injured (n = 13) human nerves. AngII levels by ELISA were also similar in control human nerves (4.09 ± 0.36 pmol/g, n = 31), injured nerves (3.99 ± 0.79 pmol/g, n = 7), and painful neuromas (3.43 ± 0.73 pmol/g, n = 12); AngIII and Ang-(1-7) levels were undetectable (<0.03 and 0.05 pmol/g respectively). Neurite lengths were significantly increased in the presence of NGF, AngII and C21 in cultured DRG neurons. AngII and, as expected, NGF significantly increased signal intensity of p38 and p42/44 MAPK, which was reversed by EMA401. AngII mediated sensitization of capsaicin responses was not observed in the presence of MAP kinase inhibitor PD98059, and the kinase inhibitor staurosporine. Conclusion The major AT2R ligand in human peripheral nerves is AngII, and its levels are maintained in injured nerves. EMA401 may act on paracrine/autocrine mechanisms at peripheral nerve terminals, or intracrine mechanisms, to reduce neuropathic pain signalling in AngII/NGF/TRPV1-convergent pathways.