bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA-seq reveals the gene expression in patterns in Populus × euramericana 'Neva' plantation under different precision water and fertilizer-intensive management
Authors: Wang Zhou , Zhang Weixi , Ding Changjun , Xia Yongxiu , Yuan Zhengsai , Guo Jiangtao , Yu Jinjin , Zhang Bingyu , Su Xiaohua 
Published: August 2024

Abstract
Background
Populus spp. is a crucial fast-growing and productive tree species extensively cultivated in the mid-latitude plains of the world. However, the impact of intensive cultivation management on gene expression in plantation remains largely unexplored.

Results
Precision water and fertilizer-intensive management substantially increased key enzyme activities of nitrogen transport, assimilation, and photosynthesis (1.12–2.63 times than CK) in Populus × euramericana 'Neva' plantation. Meanwhile, this management approach had a significant regulatory effect on the gene expression of poplar plantations. 1554 differential expression genes (DEGs)were identified in drip irrigation (ND) compared with conventional irrigation. Relative to ND, 2761–4116 DEGs, predominantly up-regulated, were identified under three drip fertilization combinations, among which 202 DEGs were mainly regulated by fertilization. Moreover, drip irrigation reduced the expression of cell wall synthesis-related genes to reduce unnecessary water transport. Precision drip and fertilizer-intensive management promotes the synergistic regulation of carbon and nitrogen metabolism and up-regulates the expression of major genes in nitrogen transport and assimilation processes (5 DEGs), photosynthesis (15 DEGs), and plant hormone signal transduction (11 DEGs). The incorporation of trace elements further enhanced the up-regulation of secondary metabolic process genes. In addition, the co-expression network identified nine hub genes regulated by precision water and fertilizer-intensive management, suggesting a pivotal role in regulating the growth of poplar.

Conclusion
Precision water and fertilizer-intensive management demonstrated the ability to regulate the expression of key genes and transcription factor genes involved in carbon and nitrogen metabolism pathways, plant hormone signal transduction, and enhance the activity of key enzymes involved in related processes. This regulation facilitated nitrogen absorption and utilization, and photosynthetic abilities such as light capture, light transport, and electron transport, which faintly synergistically regulate the growth of poplar plantations. These results provide a reference for proposing highly efficient precision intensive management to optimize the expression of target genes.
A scoping review of policies to encourage breastfeeding, healthy eating, and physical activity among rural people and places in the United States
Authors: Umstattd Meyer M. Renée , Houghtaling Bailey , Wende Marilyn E. , Kheshaifaty Khawlah H. , Delgado Haley , Eze Stephanie A. , Mecate Cassady , Summerall Woodward Rebekah , Morgan Randa Lopez , Krey Kathy J. 
Published: August 2024

AbstractBackgroundRural U.S. residents experience a disproportionate burden of diet and physical activity (PA) related chronic disease compared to urban residents, due to resource and economic challenges. Diverse policy approaches for chronic disease prevention have been implemented to address barriers to breastfeeding, healthy eating, and PA. Therefore, the purpose of this paper is to describe policy supports for breastfeeding, healthy eating, and/or PA occurring in rural U.S. areas.MethodsA scoping review was conducted March-June 2020 to identify policy, systems, and environment change approaches occurring in the rural U.S. for breastfeeding, healthy eating, and PA. Search procedures were guided by the PRISMA-ScR, Arksey and O’Malley’s work (2007), and a science librarian. Medline, PubMed, Web of Science, and Agricola were used to identify peer-reviewed research. ProQuest Dissertations and Theses A&I were used to identify dissertation research. Grey literature searches included Google, Google Scholar, government pages, and public health, federal nutrition assistance program, Cooperative Extension Services, and related webpages. Policy results are reported and inclusion criteria were: (1) breastfeeding, healthy eating, and/or PA focus; (2) about policy factors; (3) specific to U.S. rural populations/places; and (4) English language. Outcomes (study/source design, objective(s), methods/measurement, setting, population characteristics, behavioral focus, policy-specific results) were extracted into a standardized Excel document.ResultsResults include 122 total sources: original research, with some sources referencing multiple behaviors, (n = 74 sources: 8 breastfeeding, 41 healthy eating, 42 PA), grey literature (n = 45 sources: 16 breastfeeding, 15 healthy eating, 27 PA), and graduate research(n = 3 sources: 1 breastfeeding, 2 healthy eating, 1 PA). Breastfeeding policy initiatives included policies or programs at hospitals, increasing access to resources, and improving culture or norms at workplaces. Healthy eating policy initiatives included increasing access to healthy foods, reducing financial burden, implementing programs, food assistance programs, and healthy food prescriptions at healthcare facilities. PA policy initiatives focused on Complete Streets, joint or shared use efforts, Safe Routes to Schools, master plans for greenways, trails, and/or transportation, school health plans, and childcare/school standards.ConclusionsResults from this scoping review compile and offer commentary on existing policy solutions to improve breastfeeding, healthy eating, and/or PA in the rural U.S.
Risk prediction model of impacted supernumerary tooth-associated root resorption in children based on cone-beam computed tomography analysis: a case control study
Authors: Li Yakang , Zhang Yuanmin , Gao Linpei , Lyu Menghao , Zheng Baozhu , Zhang Fengqiu , Xu Junji 
Published: August 2024

Abstract
Background
External surface resorption is pressure-induced resorption and occurs on the external surface of the root, pressure exerted by impacted teeth, is common causes of external surface resorption. Predictive risk factors of impacted supernumerary tooth-associated root resorption (ISTARR) mentioned in this article include supernumerary teeth and patient factors. To investigate the risk factors of impacted supernumerary tooth-associated root resorption and predict the incidence of root resorption.

Methods
This restrospective study enrolled 324 patients with impacted supernumerary tooth. All Cone-Beam Computed Tomography (CBCT) data and patient information were divided into two groups (without tooth root resorption and with root resorption). CBCT images and patient information (age and gender) of 133 patients had adjacent tooth root resorption and 191 did not. seven variables were analysed using binary logistic regression.

Results
Individual analysis of potential risk factors showed that age, crown mesiodistal direction, root formation, and odontotheca of the impacted supernumerary tooth were associated significantly with ISTARR. Binary logistic regression showed that impacted supernumerary tooth with odontotheca (Odd Ratio = 2.926), the crown is in the middle (Odd Ratio = 1.446), located at the middle third of the adjacent tooth root (Odd Ratio = 1.614), complete root development (Odd Ratio = 1.334), and patient’s age (Odd Ratio = 1.261) were significantly associated with ISTARR risk.

Conclusions
The risk factors of root resorption can be detected and predicted early according to the features of supernumerary tooth and patient’s age. Still, more prospective studies with larger sample size are needed to validate the result.
Evaluation of the antibacterial and antifungal properties of oleuropein, olea Europea leaf extract, and thymus vulgaris oil
Authors: Al-Rimawi Fuad , Sbeih Mahmood , Amayreh Mousa , Rahhal Belal , Mudalal Samer 
Published: August 2024

Abstract
Background
Although synthetic preservatives and antioxidants may have high antimicrobial and antioxidant activity, they are usually associated with adverse effects on human health. Currently, there is a growing interest in natural antimicrobial and antioxidant agents. This study aimed to evaluate the antimicrobial activity of two medicinal plant extracts and one active compound. Olive leaf extracts (0.2, 0.3, and 0.4% w/v), oleuropein (0.2, 0.4, and 0.6% w/v), thyme oil (0.1%), and oleuropein in combination with thyme oil (0.4% w/v and 0.1% v/v) were used against three bacterial strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) and two fungal strains (Candida albicans and Aspergillus niger).

Results
The use of oleuropein resulted in complete antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. In this context, a reduction of 7 logs was achieved during the storage period (4 weeks). Oleuropein showed no fungal activity at low concentrations (0.2%), but Aspergillus niger was reduced by 2.35 logs at higher concentrations (0.6% w/v). Similar antibacterial and antifungal properties were observed for the olive leaf extracts. Oleuropein at a concentration of 0.4 w/v and a mixture of oleuropein and thyme at concentrations of 0.4 and 0.1 (v/v) showed strong antimicrobial activity against the studied microorganisms.

Conclusion
Olive leaf extract, thyme oil, and oleuropein have strong antibacterial and weak antifungal properties. There was a good synergistic effect between oleuropein and thymol.
Treatment and management of coenurosis by Taenia multiceps: field data from outbreaks in endemic regions and literature review
Authors: Abbas I. , Tamponi C. , Madau G. , Cavallo L. , Varcasia A. , Scala A. 
Published: August 2024

Abstract
Background
Taenia multiceps coenurosis is endemic in sheep from various regions worldwide. Dogs, the key hosts, shed T. multiceps eggs in their feces contaminating the pasture, and lambs are mostly infected during their first turnout into pastures. The disease is manifested in two forms: acute (due to the migrating oncospheres in the CNS) or chronic (due to the developing coenuri in the brain or spinal cord). Both forms are frequently accompanied by neurological symptoms.

Methods
Field trials conducted in an endemic region (Sardinia, Italy) to treat replacement lambs in six sheep flocks infected with acute coenurosis are summarized in this article. The article also reviews earlier reports on various approaches developed to treat and immunize sheep against coenurosis.

Results
Accurate detection of the time in which lambs become infected is crucial in deciding which treatment approach should be used. Acute disease can be successfully treated via chemotherapy. Results of field trials conducted in Sardinia revealed the efficacy of three (1-week apart) oxfendazole doses (14.15 mg/kg) in protecting apparently healthy lambs in the infected flocks from developing neurological symptoms. A single praziquantel dose (18.75 mg/kg) worked well for the same purpose and was also found significant in treating 5 of 16 clinically ill lambs in one flock. Earlier reports documented high rates of recovery (up to 100%) in clinically diseased lambs that received much higher doses (50–100 mg/kg) of praziquantel. However, chemotherapy is not preferred in chronic coenurosis since it can lead to rupture of the coenuri, giving rise to serious inflammation in the CNS. Surgical intervention is highly recommended in this case, and the pooled success rates for surgery in chronic-infected cases was estimated at 82.1% (95% CI 73.1–91.0%). However, various trials have been conducted to immunize sheep against T. multiceps coenurosis, and the 18k (Tm18) family of oncosphere antigens was found promising as a vaccine candidate.

Conclusions
In acute coenurosis, selection of the proper anthelmintic should be done after consulting the owner for several reasons: (1) costs of the used anthelmintic: treating a small flock of 100 sheep costs around 1170 and 660 € for praziquantel and oxfendazole, respectively; (2) withdrawal time of the used anthelmintic: No time is required before consuming meat and milk from praziquantel-treated sheep, whereas meat and milk from oxfendazole-treated sheep should not be consumed for 44 and 9 days, respectively, causing additional costs for the farmers. Since no commercial vaccines have yet been developed against T. multiceps coenurosis in sheep, preventive measures remain the cornerstone of controlling this serious disease.

Graphical Abstract
The genetics and epidemiology of N- and O-immunoglobulin A glycomics
Authors: Visconti Alessia , Rossi Niccolò , Bondt Albert , Ederveen Agnes Hipgrave , Thareja Gaurav , Koeleman Carolien A. M. , Stephan Nisha , Halama Anna , Lomax-Browne Hannah J. , Pickering Matthew C. , Zhou Xu-jie , Wuhrer Manfred , Suhre Karsten , Falchi Mario 
Published: August 2024

Abstract
Background
Immunoglobulin (Ig) glycosylation modulates the immune response and plays a critical role in ageing and diseases. Studies have mainly focused on IgG glycosylation, and little is known about the genetics and epidemiology of IgA glycosylation.

Methods
We generated, using a novel liquid chromatography-mass spectrometry method, the first large-scale IgA glycomics dataset in serum from 2423 twins, encompassing 71 N- and O-glycan species.

Results
We showed that, despite the lack of a direct genetic template, glycosylation is highly heritable, and that glycopeptide structures are sex-specific, and undergo substantial changes with ageing. We observe extensive correlations between the IgA and IgG glycomes, and, exploiting the twin design, show that they are predominantly influenced by shared genetic factors. A genome-wide association study identified eight loci associated with both the IgA and IgG glycomes (ST6GAL1, ELL2, B4GALT1, ABCF2, TMEM121, SLC38A10, SMARCB1, and MGAT3) and two novel loci specifically modulating IgA O-glycosylation (C1GALT1 and ST3GAL1). Validation of our findings in an independent cohort of 320 individuals from Qatar showed that the underlying genetic architecture is conserved across ancestries.

Conclusions
Our study delineates the genetic landscape of IgA glycosylation and provides novel potential functional links with the aetiology of complex immune diseases, including genetic factors involved in IgA nephropathy risk.
SGLT2 inhibitors: a novel therapy for cognitive impairment via multifaceted effects on the nervous system
Authors: Mei Jiaqi , Li Yi , Niu Liyan , Liang Ruikai , Tang Mingyue , Cai Qi , Xu Jingdong , Zhang Deju , Yin Xiaoping , Liu Xiao , Shen Yunfeng , Liu Jianping , Xu Minxuan , Xia Panpan , Ling Jitao , Wu Yuting , Liang Jianqi , Zhang Jing , Yu Peng 
Published: August 2024

AbstractThe rising prevalence of diabetes mellitus has casted a spotlight on one of its significant sequelae: cognitive impairment. Sodium-glucose cotransporter-2 (SGLT2) inhibitors, originally developed for diabetes management, are increasingly studied for their cognitive benefits. These benefits may include reduction of oxidative stress and neuroinflammation, decrease of amyloid burdens, enhancement of neuronal plasticity, and improved cerebral glucose utilization. The multifaceted effects and the relatively favorable side-effect profile of SGLT2 inhibitors render them a promising therapeutic candidate for cognitive disorders. Nonetheless, the application of SGLT2 inhibitors for cognitive impairment is not without its limitations, necessitating more comprehensive research to fully determine their therapeutic potential for cognitive treatment. In this review, we discuss the role of SGLT2 in neural function, elucidate the diabetes-cognition nexus, and synthesize current knowledge on the cognitive effects of SGLT2 inhibitors based on animal studies and clinical evidence. Research gaps are proposed to spur further investigation.
Untargeted lipidomics reveals racial differences in lipid species among women
Authors: Pourali Ghazaleh , Li Liang , Getz Kayla R. , Jeon Myung Sik , Luo Jingqin , Luo Chongliang , Toriola Adetunji T. 
Published: August 2024

AbstractUnderstanding the biological mechanisms underlying racial differences in diseases is crucial to developing targeted prevention and treatment. There is, however, limited knowledge of the impact of race on lipids. To address this, we performed comprehensive lipidomics analyses to evaluate racial differences in lipid species among 506 non-Hispanic White (NHW) and 163 non-Hispanic Black (NHB) women. Plasma lipidomic profiling quantified 982 lipid species. We used multivariable linear regression models, adjusted for confounders, to identify racial differences in lipid species and corrected for multiple testing using a Bonferroni-adjusted p-value < 10–5. We identified 248 lipid species that were significantly associated with race. NHB women had lower levels of several lipid species, most notably in the triacylglycerols sub-pathway (N = 198 out of 518) with 46 lipid species exhibiting an absolute percentage difference ≥ 50% lower in NHB compared with NHW women. We report several novel differences in lipid species between NHW and NHB women, which may underlie racial differences in health and have implications for disease prevention.
Regulating Abortion Later in Pregnancy: Fetal-Centric Laws and the Erasure of Women's Subjectivity
Authors: Kimport Katrina , Weitz Tracy A. 
Published: August 2024

Abstract
Context: In the United States, fetal development markers, including “viability” and the point when a fetus can “feel pain,” have permeated the social imaginary of abortion, affecting public support for abortion and the legality and availability of care, but the extent to which these markers describe and orient the experience of abortion at later gestations is unclear.
Methods: Using interviews with 30 cisgender women in the United States who obtained an abortion after 24 weeks of pregnancy, the authors investigated whether and how notions of fetal viability and/or pain operated in the women's lived experiences of pregnancy and abortion.
Findings: According to respondents’ accounts, fetal development–based laws restricting abortion based on purported points of fetal development operated as gestational limits, privileged the viability and pain status of the fetus over that of the prospective neonate, and failed to account for the viability and pain of the pregnant person.
Conclusions: The discursive practice of centering fetal development in regulating abortion access makes denial of abortion care because of the fetus's status conceptually available—even at the point of fertilization—and naturalizes the erasure of the subjectivity of women and others who can become pregnant.
Association of gut microbiota with depression post-myocardial infarction: A systematic evaluation and meta-analysis protocol
Authors: Liu Xiang , Shi Xiaojun , Zhao Haibin , Hou Jiqiu , Zhao Weizhe , Ding Wanli 
Published: August 2024

Background
Depression post-myocardial infarction (MI) is becoming more prevalent. The gut-brain axis (GBA), influenced by the gut microbiota, is a critical component in understanding depression post-MI. Despite the well-established connection between gut microbiota and depression post-MI, this relationship remains incompletely understood.

Methods and analysis
This protocol will follow the Preferred Reporting Items for Systematic Review and Meta-analysis Protocol (PRISMA-P) 2020 statement. Beginning from inception to October 2023, a systematic search will be conducted across eight electronic databases, including PubMed, MEDLINE, Scopus, Embase, Cochrane Clinical Trials Database, Web of Science, China National Knowledge Infrastructure, and China Biomedical Literature Database. Pre-selected studies will be independently assessed by two researchers following a standard inclusion, data extraction and quality assessment protocol. The primary outcome measures are differences in the profile of gut microbiota and rating scale scores for depression. Fixed-effects models will be used when both clinical heterogeneity and statistical heterogeneity are low, otherwise random-effects models will be used. Furthermore, subgroup analyses will be conducted on the depression severity of the participants using the same psychiatric scales employed, study type and geographic region. Random forest plot runs and research-related statistical analyses will be carried out using Rev Man V.5.3 software.

Expected results
This study will identify the association between the gut microbiota and the onset of depression post-MI, and provide evidence for the use of probiotics as an adjunctive treatment for depression post-MI.

Trial registration
Prospero registration number: CRD42023444026.
GESTÃO DE RESÍDUOS DE CONSTRUÇÃO CIVIL NOS MUNICÍPIOS DE PEQUENO PORTE DA REGIÃO TRIÂNGULO DE MINAS GERAIS
Authors: Pestana Badr Abou Dehn , Melo Nágela Aparecida de 
Published: August 2024

A gestão dos resíduos de construção civil e demolição (RCD) é um desafio para os municípios brasileiros, apesar das diretrizes do Conselho Nacional de Meio Ambiente (CONAMA) desde 2002. As dificuldades são maiores nos municípios de pequeno porte. O objetivo deste trabalho é analisar a gestão de RCD em municípios de pequeno porte da região Triângulo (MG), considerando os aspectos normativos e legais. Foram selecionados municípios com população de até 30 mil habitantes, totalizando 29 unidades. Esse estudo foi desenvolvido por meio de pesquisa documental; levantamento de dados nos planos municipais de gestão integrada de resíduos sólidos e em órgãos públicos vinculados à área ambiental. Constatou-se que na área estudada as políticas públicas e o manejo de RDC não estão em conformidade com legislações e normas vigentes. A maioria dos municípios dispõe o RCD em áreas sem controle ambiental e licenciamento. Embora os planos municipais recomendem reciclagem e reutilização, essas práticas não são aplicadas integralmente. As prefeituras não fazem o registro do quantitativo de RCD. Em razão dessa ausência, realizou-se uma estimativa do RDC gerado nos municípios estudados, os quais, em média, produzem 0,38 t/hab./ano de RCD. Portanto, é crucial adequar a gestão de RCD nos municípios pequenos conforme legislação vigente.
Approximation process of a positive linear operator of hypergeometric type
Authors: Karsli Harun 
Published: August 2024

Abstract
In this article, we construct a new sequence of positive linear operators

H

n

:
B

[

0
,
1

]

→
C

[

0
,
1

]

{H}_{n}:B{[}0,1]\to C{[}0,1]

using the hypergeometric distribution of probability theory and the rational values of f at the equally spaced control points

k
∕
n

k/n

(

k
=
0
,
1
,

…

,
n

)

\left(k=0,1,\ldots ,n)

of the unit interval [0,1]. Moreover, we obtain some approximation properties of these operators. It is important to note that hypergeometric distribution has a special interest in probability theory because of its natural behaviour. Namely, unlike all other discrete distributions, the previous steps in the hypergeometric distribution affect the next steps. In other discrete distributions, the process starts from the beginning at each stage, whereas in the hypergeometric distribution, the previous steps determine the structure of the next steps, since the previous steps are not replaced.
On large deviations of the moment of attaining far level by the random walk in a random environment
Authors: Bakay Gavriil A. 
Published: August 2024

Abstract
We prove local limit theorems on large deviations for the moment T

n
of attaining the level n ∈ ℕ by the random walk in a random environment. Exact asymptotics of probabilities P(Tn
= k) are obtained for values of the parameter k corresponding to the large deviations zone.