bluuebunny/crossref_metadata_2025_split huggingface dataset:
Do male secondary sexual characters signal ejaculate quality? A meta‐analysis
Authors: Mautz Brian S. , Møller Anders P. , Jennions Michael D. 
Published: February 2013

ABSTRACTThere are two reasons why researchers are interested in the phenotypic relationship between the expression of male secondary sexual characters (SSCs) and ‘ejaculate quality’ (defined as sperm/ejaculate traits that are widely assumed to increase female fertility and/or sperm competitiveness). First, if the relationship is positive then females could gain a direct benefit by choosing more attractive males for fertility assurance reasons (‘the phenotype‐linked fertility’ hypothesis). Second, there is much interest in the direction of the correlation between traits favoured by pre‐copulatory sexual selection (i.e. affecting mating success) and those favoured by post‐copulatory sexual selection (i.e. increasing sperm competitiveness). If the relationship is negative this could lead to the two forms of selection counteracting each other. Theory predicts that the direction of the relationship could be either positive or negative depending on the underlying genetic variance and covariance in each trait, the extent of variation among males in condition (resources available to allocate to reproductive traits), and variation among males in the cost or rate of mating. We conducted a meta‐analysis to determine the average relationship between the expression of behavioural and morphological male secondary sexual characters and four assays of ejaculate quality (sperm number, viability, swimming speed and size). Regardless of how the data were partitioned the mean relationship was consistently positive, but always statistically non‐significant. The only exception was that secondary sexual character expression was weakly but significantly positively correlated with sperm viability (r = 0.07,P < 0.05). There was no significant difference in the strength or direction of the relationship between behavioural and morphologicalSSCs, nor among relationships using the four ejaculate quality assays. The implications of our findings are discussed.
Snails and their trails: the multiple functions of trail‐following in gastropods
Authors: Ng Terence P. T. , Saltin Sara H. , Davies Mark S. , Johannesson Kerstin , Stafford Richard , Williams Gray A. 
Published: February 2013

AbstractSnails are highly unusual among multicellular animals in that they move on a layer of costly mucus, leaving behind a trail that can be followed and utilized for various purposes by themselves or by other animals. Here we review more than 40 years of experimental and theoretical research to try to understand the ecological and evolutionary rationales for trail‐following in gastropods. Data from over 30 genera are currently available, representing a broad taxonomic range living in both aquatic and terrestrial environments. The emerging picture is that the production of mucus trails, which initially was an adaptation to facilitate locomotion and/or habitat extension, has evolved to facilitate a multitude of additional functions. Trail‐following supports homing behaviours, and provides simple mechanisms for self‐organisation in groups of snails, promoting aggregation and thus relieving desiccation and predation pressures. In gastropods that copulate, trail‐following is an important component in mate‐searching, either as an alternative, or in addition to the release of water‐ or air‐borne pheromones. In some species, this includes a capacity of males not only to identify trails of conspecifics but also to discriminate between trails laid by females and males. Notably, trail discrimination seems important as a pre‐zygotic barrier to mating in some snail species. As production of a mucus trail is the most costly component of snail locomotion, it is also tempting to speculate that evolution has given rise to various ways to compensate for energy losses. Some snails, for example, increase energy intake by eating particles attached to the mucus of trails that they follow, whereas others save energy through reducing the production of their own mucus by moving over previously laid mucus trails. Trail‐following to locate a prey item or a mate is also a way to save energy. While the rationale for trail‐following in many cases appears clear, the basic mechanisms of trail discrimination, including the mechanisms by which many snails determine the polarity of the trail, are yet to be experimentally determined. Given the multiple functions of trail‐following we propose that future studies should adopt an integrated approach, taking into account the possibility of the simultaneous occurrence of many selectively advantageous roles of trail‐following behaviour in gastropods. We also believe that future opportunities to link phenotypic and genotypic traits will make possible a new generation of research projects in which gastropod trail‐following, its multitude of functions and evolutionary trade‐offs can be further elucidated.
Anti-tumour necrosis factor therapy enhances mucosal healing through down-regulation of interleukin-21 expression and T helper type 17 cell infiltration in Crohn's disease
Authors: Liu C , Xia X , Wu W , Wu R , Tang M , Chen T , Xu F , Cong Y , Xu X , Liu Z 
Published: February 2013

Summary
Anti-tumour necrosis factor (TNF) monoclonal antibody (mAb) (infliximab, IFX) has been shown to be highly effective in the management of Crohn's disease (CD). Herein we investigated the potential role of IFX in inducing clinical remission and regulating interleukin (IL)-21 expression and T helper type 17 (Th17) cell infiltration in the intestinal mucosa of CD patients. Twenty-six CD patients were treated with IFX at weeks 0, 2 and 6. Clinical response, mucosal healing, serum C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) were evaluated at week 10 after IFX administration. Expression of IL-21, IL-17A and retinoic acid-related orphan receptor C (RORC) in intestinal mucosa were analysed by quantitative real-time polymerase chain reaction (PCR) and immunohistochemistry. Peripheral blood and lamina propria CD4+ T cells were stimulated with anti-CD3 and anti-CD28 mAbs in the presence of IFX. Cytokine profiles and RORC were determined with enzyme-linked immunosorbent assay (ELISA) and real-time PCR. IL-21 and Th17 cells were found to be expressed highly in inflamed mucosa of active CD patients compared with healthy controls. Ten weeks after IFX infusion, CD activity index, ESR, CRP and intestinal mucosal healing were improved markedly in CD patients, and IL-21 expression and Th17 cell infiltration were decreased significantly compared with those before IFX therapy. In-vitro study demonstrated that IFX treatment could suppress IL-21, IL-17A and RORC expression in cultured CD biopsies. Moreover, IFX was also observed to down-regulate markedly IL-17A, IL-21 and RORC expression by CD CD4+ T cells. IFX is highly effective in inducing clinical remission and promoting intestinal mucosal healing in CD patients through down-regulation of IL-21 expression and Th17 cell infiltration in intestinal mucosa.
Symmetry in 3D Geometry: Extraction and Applications
Authors: Mitra Niloy J. , Pauly Mark , Wand Michael , Ceylan Duygu 
Published: February 2013

AbstractThe concept of symmetry has received significant attention in computer graphics and computer vision research in recent years. Numerous methods have been proposed to find, extract, encode and exploit geometric symmetries and high‐level structural information for a wide variety of geometry processing tasks. This report surveys and classifies recent developments in symmetry detection. We focus on elucidating the key similarities and differences between existing methods to gain a better understanding of a fundamental problem in digital geometry processing and shape understanding in general. We discuss a variety of applications in computer graphics and geometry processing that benefit from symmetry information for more effective processing. An analysis of the strengths and limitations of existing algorithms highlights the plenitude of opportunities for future research both in terms of theory and applications.
Exploring the profile of articles on traumatic dental injuries in pediatric dental journals
Authors: Feldens Carlos Alberto , Kramer Paulo Floriani , Feldens Eliane Gerson 
Published: February 2013

AbstractAimTo explore the profile of articles on traumatic dental injuries (TDI) published in leading pediatric dental journals between 2000 and 2010 via bibliometric analysis.MethodsA search was conducted on the PubMed database to find the six pediatric dental journals with the highest numbers of articles on TDI published between January 2000 and December 2010. All titles and abstracts of full‐length articles were reviewed by two examiners. Studies focusing on TDI were independently categorized according to the following aspects: (i) year of publication; (ii) country where the study was carried out; (iii) study design; (iv) topic addressed; (v) type of TDI; and (vi) type of dentition. Data were analyzed in terms of frequency distribution.ResultsOf a total of 3720 articles published in the six selected journals, only 119 (3.2%) focused on TDI, with no significant variations across the journals or years. The countries with the greatest number of publications on TDI were India (19.3%), followed by the USA (15.1%), Brazil (13.4%), and Italy (11.8%). Case report and case series were the dominant study design (53%). Most studies addressed treatment of TDI (63%) and described injuries to permanent teeth (68%), especially avulsion and crown fractures.ConclusionsThere is an urgent need for expanding the number and quality of research on TDI in pediatric dental journals, especially with better quality study designs. Cohort studies and investigations focusing on the assessment of health services and technologies are important targets for future research.
Decoding face categories in diagnostic subregions of primary visual cortex
Authors: Petro Lucy S. , Smith Fraser W. , Schyns Philippe G. , Muckli Lars 
Published: February 2013

AbstractHigher visual areas in the occipitotemporal cortex contain discrete regions for face processing, but it remains unclear if V1 is modulated by top‐down influences during face discrimination, and if this is widespread throughout V1 or localized to retinotopic regions processing task‐relevant facial features. Employing functional magnetic resonance imaging (fMRI), we mapped the cortical representation of two feature locations that modulate higher visual areas during categorical judgements – the eyes and mouth. Subjects were presented with happy and fearful faces, and we measured the fMRI signal of V1 regions processing the eyes and mouth whilst subjects engaged in gender and expression categorization tasks. In a univariate analysis, we used a region‐of‐interest‐based general linear model approach to reveal changes in activation within these regions as a function of task. We then trained a linear pattern classifier to classify facial expression or gender on the basis of V1 data from ‘eye’ and ‘mouth’ regions, and from the remaining non‐diagnostic V1 region. Using multivariate techniques, we show that V1 activity discriminates face categories both in local ‘diagnostic’ and widespread ‘non‐diagnostic’ cortical subregions. This indicates that V1 might receive the processed outcome of complex facial feature analysis from other cortical (i.e. fusiform face area, occipital face area) or subcortical areas (amygdala).
Malignant melanoma of the third eyelid in a horse
Authors: Albanese V. , Newton J. C. , Waguespack R. W. 
Published: February 2013

SummaryAn 8‐year‐old greyQuarterHorse gelding was referred for evaluation of a rapidly growing mass associated with the third eyelid of the left eye. A pigmented mass of approximately 2 cm in diameter was palpated and visualised associated with the conjunctival lining of the nictitans. It was not possible to palpate normal nictitans deep to the base of the mass. A full dermatological examination revealed no other melanomas in common sites. Based on the size and rapid growth of the mass, surgical excision and one application of local chemotherapy was performed under general anaesthesia. Histopathology confirmed the diagnosis of malignant melanoma and the presence of clean surgical margins. There was no recurrence at 5 weeks post surgery. To our knowledge, this is the first report of a primary malignant melanoma of the third eyelid in a horse.
Intracellular accumulation of advanced glycation end products induces apoptosis via endoplasmic reticulum stress in chondrocytes
Authors: Yamabe Soichiro , Hirose Jun , Uehara Yusuke , Okada Tatsuya , Okamoto Nobukazu , Oka Kiyoshi , Taniwaki Takuya , Mizuta Hiroshi 
Published: February 2013

Mammalian cells attempt to maintain their homeostasis under endoplasmic reticulum (ER) stress. If the stress cannot be alleviated, cells are led to apoptosis through induction of C/EBP homologous protein (CHOP). ER stress is provoked in osteoarthritis chondrocytes, and intracellular accumulation of advanced glycation end products (AGEs) in chondrocytes is a possible cause. To clarify the role of intracellular AGE accumulation in chondrocytes, the present study investigated the effect of intracellular AGE accumulation on ER stress and apoptosis by in vitro and in vivo analysis. Intracellular AGE accumulation induced by AGE precursors caused apoptosis, induced expression of ER stress markers, and led to co‐localization of AGEs with glucose‐regulated protein 78, leading to formation of high‐molecular‐weight complexes in cultured chondrocytes. These reactions were inhibited by an AGE formation inhibitor. CHOP deletion inhibited apoptosis induced by intracellular AGE accumulation. In vivo intracellular AGE accumulation induced by intra‐articular injection of AGE precursors caused ER stress and apoptosis in chondrocytes and led to degradation of articular cartilage. Additionally, intracellular AGE accumulation increased the degree of cartilage degradation in an osteoarthritis model. These data indicate that intracellular accumulation of AGEs induces modification of unfolded protein response‐related protein by AGEs and apoptosis via ER stress in chondrocytes. Moreover, the in vivo study showed that intracellular AGE accumulation in chondrocytes is involved in the occurrence and progression of osteoarthritis through ER stress. Thus, research on mechanisms of apoptosis via ER stress induced by intracellular AGE accumulation in chondrocytes will lead to a new understanding of osteoarthritis pathology.
Underestimation of primary productivity on continental shelves: evidence from maximum size of extant surfclam (Spisula solidissima) populations
Authors: Munroe D.M. , Powell E.N. , Mann R. , Klinck J.M. , Hofmann E.E. 
Published: February 2013

AbstractAtlantic surfclams (Spisula solidissima), among the largest extant non‐symbiotic clam species in the world, live in dense aggregations along the Middle Atlantic Bight (MAB) continental shelf. The food resources that support these populations are poorly understood. An individual‐based model that simulates the growth of post‐settlement surfclams was used to investigate the quantity of food needed to maintain existing surfclam populations along the MAB continental shelf. Food inputs to the model were based on measured near‐bottom water‐column chlorophyll concentrations. Simulations showed that these water‐column food sources supported only 65% of the observed body mass of a standard large surfclam (160‐mm shell length). Additional simulations using benthic food sources to supplement water‐column food sources by 20% generated surfclams that grew to observed size and biomass and exhibited spawn timing consistent with the known surfclam spawning season. The simulation results suggest that measured water‐column chlorophyll concentrations may underestimate the food available to the continental shelf benthos. Large continental shelf bivalves are an essential resource for fisheries and higher trophic level consumers. Understanding available and utilized food resources is important for predicting long‐term impacts of climate change on benthic secondary production and fishery yield on the continental shelf.
Vertical behavior of bigeye tuna (Thunnus obesus) in the northwestern Pacific Ocean based on archival tag data
Authors: Matsumoto Takayuki , Kitagawa Takashi , Kimura Shingo 
Published: February 2013

AbstractThe behavior of bigeye tuna (Thunnus obesus) in the northwestern Pacific Ocean was investigated using archival tag data for 28 fish [49–72 cm fork length (FL) at release, 3–503 days] released in Japanese waters around the Nansei Islands (24–29°N, 122–132°E) and east of central Honshu (Offshore central Honshu, 32–36°N, 142–148°E). Vertical behavior was classified into three types based on past studies: ‘characteristic’ (non‐associative), ‘associative’ (associated with floating objects) and ‘other’ (behavior not fitting into these two categories). The proportion of fish showing associative behavior decreased and that of characteristic behavior increased as fish grew, and this shift was pronounced at 60–70 cm FL. The fish usually stayed above the 20°C isotherm during the daytime and nighttime when showing associative behavior and below the 20°C isotherm during daytime for characteristic behavior. A higher proportion of characteristic behavior was seen between December and April around the Nansei Islands, and between September and December for offshore central Honshu. Seasonal changes in vertical position were also observed in conjunction with changes in water temperature. In this study, ‘other’ behavior was further classified into five types, of which ‘afternoon dive’ behavior, characterized by deep dives between around noon and evening, was the most frequent. The present study indicated that in the northwestern Pacific Ocean, the vertical behavior of bigeye tuna changes with size, as well as between seasons and regions.
Atmospheric forcing drives recruitment variation in the Dungeness crab (Cancer magister), revisited
Authors: Shanks A. L. 
Published: February 2013

AbstractFor 12 yr (1997–2001, 2006–2012) daily abundance of Cancer magister megalopae was measured in Coos Bay, Oregon. Before 2007 from 2000 to 80 000 megalopae were caught annually. In 2007, catch jumped and has since varied from 164 000 to 2.3 million. The step change in catch size appears related to a shift to negative Pacific Decadal Oscillation (PDO) values. Late season catches, which cannot be due to local spawning, are negatively correlated to the PDO, suggesting that these megalopae derive from north of the California Current. During periods of lower and higher catches, annual returns of megalopae were significantly negatively correlated to the day of the year of the spring transition and positively correlated to the amount of upwelling during the settlement season. The size of the Oregon commercial catch lagged 4 yr to allow for growth of recruits into the fishery is set by the number of returning megalopae; the relationship is parabolic. At lower returns, the population is recruitment limited, but at higher returns, density‐dependent effects predominate and set the commercial catch. Lagged commercial catches in Washington and Northern and Central California were also related to the number of megalopae returning to Coos Bay, suggesting that the forces causing variation in larval success are coast wide. If high return rates are due to a PDO regime shift, then for years to decades the commercial catch may be set by density‐dependent effects following settlement and the huge numbers of returning megalopae may impact benthic community structure.
Functional limitations in Romanian children with haemophilia: further testing of psychometric properties of the Paediatric Haemophilia Activities List
Authors: Groen W. , van der Net J. , Lacatusu A. M. , Serban M. , Helders P. J. M. , Fischer K. 
Published: February 2013

SummaryChildren with haemophilia often experience limitations in activities of daily life. Recently the Paediatric Haemophilia Activities List (PedHAL) has been developed and tested in Dutch children with intensive replacement therapy. The psychometric properties of the PedHAL in children not receiving intensive replacement therapy are not known. The objective was to gain further insight into the psychometric properties of the PedHAL and to study the functional health status of Romanian children and adolescents with haemophilia. Children attending to the rehabilitation centre of Buzias in Romania were sampled consecutively. Construct validity of the PedHAL was evaluated by concurrent testing with objective and subjective measures of physical function and functional ability. Reproducibility was tested by a 3‐day test–retest by intraclass correlation coefficient (ICC) and limits of agreement (LOA). Responsiveness to rehabilitation was assessed by Haemophilia Joint Health Score (HJHS) and PedHAL. Twenty‐nine children with severe (n = 25) or moderate (n = 4) haemophilia participated. Mean age was 13.2 years (SD 4.0). Median score of the PedHAL was 83.5 (IQR 47.9–90.5). The PedHAL correlated moderately with HJHS (rho = −0.59), Functional Independence Score in& Haemophilia (rho = 0.65) and Child Health Questionnaire‐physical function (rho = 0.40) and not with Child Health Questionnaire‐mental health, Child Health Questionnaire‐behaviour and 6MWT. Test‐retest reliability was good (ICC = 0.95). LOA was 17.4 points for the sum score. HJHS scores improved slightly after rehabilitation, whereas PedHAL scores did not change. In general, construct validity and test–retest reliability were good, test–retest agreement showed some variability. Therefore, currently the PedHAL may be more appropriate for research purposes than for individual patient monitoring in clinical practice.
Practical and cost‐effective measurement of B‐domain deleted and full‐length recombinant FVIII in the routine haemostasis laboratory
Authors: Cauchie M. , Toelen J. , Peerlinck K. , Jacquemin M. 
Published: February 2013

SummaryThe assessment of recombinant FVIII (rFVIII) activity (FVIII:C) in plasma of patients is dependent on the assay. Notably, a calibration with a product‐specific laboratory standard is recommended when measuring Refacto‐AFR activity in plasma with a one‐stage assay. The objective of this study was to facilitate the measurement of rFVIII, taking into account the recent demonstration that a calibration curve does not have to be included in each run. FVIII:C was measured in patients' samples after infusion of different types of rFVIII with a one‐stage and a chromogenic assay calibrated either with pooled normal plasma or a product‐specific laboratory standard. Results obtained with the one‐stage coagulation assay were compared with these provided by a chromogenic assay. We confirmed that a calibration curve can be used for a prolonged period of time without loss of precision and accuracy. In such conditions, a stable relation between the calibration curves generated with a product‐specific laboratory standard and plasma can be established. In patients' plasma, Refacto‐AF levels measured with a one‐stage FVIII assay calibrated with plasma or a product‐specific laboratory standard diverged from −58% to −17% and from −25% to +18%, respectively, from the activity determined with a chromogenic substrate assay. By comparison, FVIII:C levels of full‐length rFVIII measured with the one‐stage assay calibrated with plasma were 6–49% lower than with the chromogenic assay. In a monocentric setting, the long‐term stability of the calibration curves allows the implementation of a practical and cost‐effective approach to determine rFVIII:C levels.