bluuebunny/crossref_metadata_2025_split huggingface dataset:
A genotyping protocol for multiple tissue types from the polyploid tree species Sequoia sempervirens (Cupressaceae)
Authors: Narayan Lakshmi , Dodd Richard S. , O'Hara Kevin L. 
Published: March 2015

Premise of the study:Identifying clonal lineages in asexually reproducing plants using microsatellite markers is complicated by the possibility of nonidentical genotypes from the same clonal lineage due to somatic mutations, null alleles, and scoring errors. We developed and tested a clonal identification protocol that is robust to these issues for the asexually reproducing hexaploid tree species coast redwood (Sequoia sempervirens).Methods:Microsatellite data from four previously published and two newly developed primers were scored using a modified protocol, and clones were identified using Bruvo genetic distances. The effectiveness of this clonal identification protocol was assessed using simulations and by genotyping a test set of paired samples of different tissue types from the same trees.Results:Data from simulations showed that our protocol allowed us to accurately identify clonal lineages. Multiple test samples from the same trees were identified correctly, although certain tissue type pairs had larger genetic distances on average.Discussion:The methods described in this paper will allow for the accurate identification of coast redwood clones, facilitating future studies of the reproductive ecology of this species. The techniques used in this paper can be applied to studies of other clonal organisms as well.
Femtosecond Laser-Assisted Cataract Surgery Without OVD: A Prospective Intraindividual Comparison
Authors: Schargus Marc , Suckert Nathanael , Schultz Tim , Kakkassery Vinodh , Dick H. Burkhard 
Published: March 2015

PURPOSE:
To compare femtosecond laser-assisted cataract surgery without the use of ophthalmic viscosurgical device (OVD) with standard phacoemulsification using OVD.

METHODS:
This was a prospective, randomized, single-center trial of 37 patients (74 eyes) diagnosed as having significant cataract in both eyes. Randomly, one eye underwent standard phacoemulsification with OVD (the OVD group); this group acted as controls. The other eye was treated with the femtosecond laser; the subsequent manual part of the procedure was performed without OVD (non-OVD group). Time of surgery, amount of fluid used during phacoemulsification, central corneal thickness, intraocular pressure, endothelial cell count, and visual acuity were documented over the 6-month follow-up.

RESULTS:

There were no major complications and no significant difference in overall surgery time (non-OVD: 375 ± 81 seconds; OVD: 362 ± 43 seconds;
P
= .713) and in the quantity of fluid passing through the eye (non-OVD: 187 ± 35 mL; OVD: 186 ± 27 mL;
P
= .952) between groups. Endothelial cell loss after 6 months was not significantly different between groups (non-OVD: −2.4%; OVD: −2.7%;
P
= .880). Central corneal thickness was not different at 1 week postoperatively between groups (non-OVD: 575 ± 45
µ
m; OVD: 573 ± 46
µ
m;
P
= .820). Three patients in the OVD group and one patient in the non-OVD group experienced intraocular pressure greater than 25 mm Hg at 1 day postoperatively. There were no significant differences in corrected distance visual acuity between groups (logMAR non-OVD: 0.024; OVD: 0.038;
P
= .461).

CONCLUSIONS:
Femtosecond laser-assisted treatment allows the cataract surgeon to perform phacoemulsification and intraocular lens implantation without the use of OVD at no additional risk to the corneal endothelium. Furthermore, there was a tendency toward fewer increases in intraocular pressure in patients treated with the femtosecond laser.

[
J Refract Surg
. 2015;31(3):146–152.]
Initial Clinical Results of a New Telescopic IOL Implanted in Patients With Dry Age-Related Macular Degeneration
Authors: Hengerer Fritz H. , Artal Pablo , Kohnen Thomas , Conrad-Hengerer Ina 
Published: March 2015

PURPOSE:
To evaluate the safety and efficacy of the iol-AMD technology (London Eye Hospital Pharma, London, UK), which includes two injectable, hydrophobic acrylic intraocular lenses (IOLs) in a pilot study of patients diagnosed as having cataract and dry age-related macular degeneration.

METHODS:
The cataract surgery and IOL implantation were performed after a preoperative evaluation using the iolAMD simulator in eyes with bilateral intermediate dry age-related macular degeneration. Outcomes were intraoperative and postoperative complications, subjective and objective visual acuity improvement, visual field changes, and postoperative diplopia.

RESULTS:
Three eyes of 2 patients were evaluated. The surgeries were uneventful. All eyes gained monocular reading vision at the 1-week postoperative visit. One patient with monocular implantation recognized diplopia for distance vision. Preoperative corrected distance visual acuity ranged from 20/800 to 20/125 and corrected near visual acuity was 20/800 or less. Two months after surgery, corrected distance and near visual acuities increased to levels between 20/40 and 20/25 (uncorrected distance visual acuity was 20/60 to 20/32; uncorrected near visual acuity was 20/200 to 20/25).

CONCLUSIONS:
These early results showed that the iolAMD simulator is a promising technology improving near and distance visual acuity in eyes with intermediate dry macular degeneration. The prismatic IOL effect did not lead to diplopia when implanted bilaterally. The surgery was safely performed.

[
J Refract Surg
. 2015;31(3):158–162.]
LASIK Ablation Centration: An Objective Digitized Assessment and Comparison Between Two Generations of an Excimer Laser
Authors: Kanellopoulos Anastasios John , Asimellis George 
Published: March 2015

PURPOSE:
To objectively define the effective centration of myopic femtosecond laser-assisted LASIK ablation pattern, evaluate the difference between achieved versus planned excimer laser ablation centration, and compare these results from two different generations of an excimer laser system.

METHODS:
The study retrospectively evaluated 280 eyes subjected to myopic LASIK. Digital image analysis was performed on Scheimpflug sagittal curvature maps (difference of preoperative to postoperative). Centration was assessed via proprietary software digital analysis of the coordinate displacement between the achieved ablation geometric center and the planned ablation center, which was the corneal vertex. Results from two different excimer laser generations (Eye-Q 400 [140 eyes] and EX500 [140 eyes]; Alcon/WaveLight, Fort Worth, TX) were compared.

RESULTS:

Radial displacement was on average 360 ± 220
µ
m (range: 0 to 1,030
µ
m) in the Eye-Q 400 laser group and 120 ± 110
µ
m (range: 0 to 580
µ
m) in the EX500 laser group (
P
< .01). The percentage of eyes with displacement of greater than 300
µ
m was 52% in the Eye-Q 400 laser group and 4% in the EX500 laser group.

CONCLUSIONS:
Displacement of ablation pattern may depend on the laser platform used. The improvement in the efficiency of centration indicates that newer generation excimer lasers with faster eye tracking and active centration control appear to achieve a significantly more accurate centration of myopic ablation patterns. The authors propose this novel, objective technique for laser refractive surgeon evaluation may point out significant outcome measures not currently used in standard metrics of refractive laser efficiency.

[
J Refract Surg.
2015;31(3):164–169.]
Evaluation of a New Controlled Point Source LED Glare Tester for Disability Glare Detection in Participants With and Without Cataracts
Authors: Epitropoulos Alice T. , Fram Nicole R. , Masket Samuel , Price Francis W. , Snyder Michael E. , Stulting R. Doyle 
Published: March 2015

PURPOSE:
To determine glare-induced change during visual acuity testing in patients with and without cataract using the controlled point source light-emitting diode (LED) glare tester (EpiGlare Tester; Epico, LLC, Columbus, OH), a new medical device for identification of glare disability.

METHODS:
This prospective, multicenter study enrolled 40 patients (80 eyes with cataracts) and 49 control subjects (98 eyes without cataracts). Corrected distance visual acuity (CDVA) was measured with and without glare using the EpiGlare Tester as a glare source. Functional visual ability was evaluated using driving and glare subscales from the Refractive Status Vision Profile questionnaire. The primary efficacy measure was change in CDVA measurement with and without glare in patients with senile cataract compared to participants without cataract. Secondary efficacy measures included correlation of the CDVA change caused by functional glare disability and subjective patient and investigator assessments.

RESULTS:

CDVA reduction was greater for patients with cataract, with a mean reduction of −0.49 ± 0.3 logMAR, than for participants without cataracts at −0.13 ± 0.2 logMAR (
P
< .001). This equates to a 5-line Snellen reduction (0.49 logMAR) in patients with cataracts and a 1-line reduction (−0.13 logMAR) in patients without cataracts. Among patients with cataracts, 83% stated the device accurately represented the difficulty experienced while driving at night (
P
< .001); among participants without cataracts, 71% reported being minimally affected by glare from the device (
P
= .003).

CONCLUSIONS:
A new controlled point source LED glare tester demonstrated the adverse effect on visual acuity due to glare in patients with cataract, accurately simulated night driving glare issues for patients with cataracts, and was rated as easy to use and useful by investigators.

[
J Refract Surg.
2015;31(3):196–201.]
Wang-Landau algorithm for entropic sampling of arch-based microstates in the volume ensemble of static granular packings
Authors: Slobinsky Demian , Pugnaloni Luis Ariel 
Published: March 2015

We implement the Wang-Landau algorithm to sample with equal probabilities the static configurations of a model granular system. The "non-interacting rigid arch model" used is based on the description of static configurations by means of splitting the assembly of grains into sets of stable arches. This technique allows us to build the entropy as a function of the volume of the packing for large systems. We make a special note of the details that have to be considered when defining the microstates and proposing the moves for the correct sampling in these unusual models. We compare our results with previous exact calculations of the model made at moderate system sizes. The technique opens a new opportunity to calculate the entropy of more complex granular models.Received: 19 January 2015, Accepted: 25 February 2015; Reviewed by: M. Pica Ciamarra, Nanyang Technological University, Singapore; Edited by: C. S. O'Hern; DOI: http://dx.doi.org/10.4279/PIP.070001Cite as: D Slobinsky, L A Pugnaloni, Papers in Physics 7, 070001 (2015) This paper, by Demian Slobinsky, Luis Ariel Pugnaloni , is licensed under the Creative Commons Attribution License 3.0.
Study of Anemia in Antenatal Care Patients: A Retrospective Study
Authors: Mandve Priyanka , Nawale Kundan , Motghare Vijay M , Pajai Sandhya 
Published: March 2015

ABSTRACT

Aim
To study the prevalence of anemia in antenatal care (ANC) patients and their sociodemographic factors associated with anemia.

Materials and methods
In this retrospective hospital-based study, data were collected from medical record section from 1st January to 31st December 2011. Patients admitted to labour room for delivery were selected as per the WHO classification of anemia. Various sociodemografic factors were also studied. Data were analyzed with the help of frequency and percentage tables. Association between variables were checked by Chisquare test.

Results
Out of 6,356 patients delivered, 6,300 patients were anemic. Maximum percentage of patients (93.8%) were in the category of moderate anemia. A total of 4174 patients were in age group of 21 to 25 years. It was found that 56.4% of patients are primigravida. Seventy-eight percent patients belong to rural population. Baby weight of 3,046 patients were in group of 2.1 to 2.5 kg.

Conclusion
It was found that out of 6356 patients delivered, 6300 were anemic. Maximum patients (93.8%) had moderate anemia, mild and severe anemia was found in 0.8 and 5.4% of patients.

How to cite this article
Mandve P, Nawale K, Motghare VM, Pajai S. Study of Anemia in Antenatal Care Patients: A Retrospective Study. J South Asian Feder Obst Gynae 2014; 6(3):133-138.
Prevalence of Human Immunodeficiency Virus in Antenatal Mothers in a Rural Tertiary Care Hospital
Authors: Sarkar Dhrubajyoti , Bhadra Banasree , Sarkar Suvobrata , Chakraborty Biswajit 
Published: March 2015

ABSTRACT

Objective
To know the prevalence of human immunodeficiency virus (HIV) among antenatal mothers.

Materials and methods
It is a retrospective study of antenatal mothers attending the integrated counseling and testing centre (ICTC), College of Medicine and JNM Hospital, Kalyani, from January 2009 to December 2013. Antenatal mothers were counseled and in those willing to undergo test rapid testing was done. First time positive cases were retested two more times by two different companies rapid test kit. The samples were considered as positive when found reactive by all three different methods.

Results
In our study, a total number of 11343 new antenatal mothers were registered. The pretest counseling could be done for 9437 antenatal mothers. After counseling, testing could be done in 9211 antenatal mothers. In our 5 years study period, a total of six cases were HIV positive. Out of these HIV positive cases, three were in 2009, no case was detected in 2010, one was in 2011, one in 2012 and one in 2013. Thus, the incidence of HIV (in tested mothers) is 0.13% in 2009, 0 in 2010, 0.06% in 2011, 0.07% in 2012 and 0.05% in 2013. Overall incidence was 0.065%.

Conclusion
There is a declining trend of HIV prevalence among antenatal mothers reflecting that prevention campaigns are working effectively.

How to cite this article
Bhadra B , S arkar D , S arkar S ,Chakraborty B. Prevalence of Human Immunodeficiency Virus in Antenatal Mothers in a Rural Tertiary Care Hospital. J South Asian Feder Obst Gynae 2014;6(3):159-162.
Laparoscopic Management of Interstitial Ectopic Pregnancies
Authors: Desai Shyam V , Desai Gaurav S , Levi Jessi 
Published: March 2015

ABSTRACT
This illustration describes the successful laparoscopic management of interstitial ectopic pregnancy. Three women underwent laparoscopic excision for interstitial ectopic gestation. Mean operative time was 49.4 ± 8.4 minutes (41- 69 mins). Estimated blood loss was 50 ± 4 ml. There were no intraoperative or postoperative complications. Duration of hospital stay was 24 ± 3 hours. All patients are doing well on follow-up. The authors demonstrate the role of laparoscopic excision and conclude that this technique, when performed by experienced surgeons, allows for improved dexterity and is a safe and effective method in the management of interstitial ectopic pregnancy.

How to cite this article
Desai GS, Levi J, Desai SV. Laparoscopic Management of Interstitial Ectopic Pregnancies. J South Asian Feder Obst Gynae 2014;6(3):163-166.
Double Trouble: Heterotopic Pregnancy
Authors: Krishnamoorthy Shruthi , Rani G Usha , Syamala O , LNU Rukshana , Alexander Naveen 
Published: March 2015

ABSTRACTA heterotopic pregnancy is defined as the presence of a combined intrauterine and ectopic pregnancy. Its estimated incidence is as between 1/7000 and 1/30,000 pregnancies. It is also reported to be as high as 1% after the use of assisted reproductive technology. Heterotopic pregnancies are diagnostic and therapeutic challenges for obstetricians. If they continue without diagnosis, a life-threatening situation may occur even when timely surgical intervention with laparotomy is performed. Here, we report a case series of three patients having three different scenarios, who were diagnosed with heterotopic pregnancies in the first trimester and managed successfully.How to cite this articleKrishnamoorthy S, Rani GU, Syamala O, Rukshana, Alexander N. Double Trouble: Heterotopic Pregnancy J South Asian Feder Obst Gynae 2014;6(3):167-170.
The Public and Private Lives of Mennonite Kolkhoz Chairmen in the Khortytsia and Molochansk German National Raĭony in Ukraine (1928-1934)
Authors: Neufeldt Colin P. 
Published: March 2015

This article examines the role that Mennonites played in the establishment and management of kolkhozy in the two largest Mennonite settlements (Khortytsia and Molochansk) in Soviet Ukraine during dekulakization and collectivization (1928-1934). More specifically, it investigates the social and ethnic criteria used in selecting Mennonites to be kolkhoz chairmen; the duties and daily routines of chairmen; the conflicted relationships that chairmen had with local authorities and kolkhoz members; the numerous challenges that chairmen encountered during the 1932-33 famines; and the mechanisms that local authorities and kolkhoz members used to control, embarrass, and discipline chairmen. It also discusses the negative repercussions that the rise of Nazi Germany had for Mennonite chairmen, and how political, economic, agricultural, social, and ethnic policies and conditions made it impossible for Mennonite chairmen to succeed.
One-Year Efficacy of the RUDI Technique for Flow Reduction in High-Flow Autologous Brachial Artery-Based Hemodialysis Vascular Access
Authors: Vaes Roel H.D. , van Loon Magda , Vaes Selma M.M. , Cuypers Philippe , Tordoir Jan H. , Scheltinga Marc R. 
Published: March 2015

Purpose Flow reduction is advised in hemodialysis (HD) patients with a high-flow (>2 L/min) arteriovenous fistula (AVF). The revision using distal inflow (RUDI) technique is based on the premise that access flow is attenuated once inflow is provided by a smaller caliber forearm artery. Aim of the study was to evaluate the efficacy of RUDI during a 1-year follow-up. Methods All HD patients undergoing a RUDI operation using a greater saphenous vein (GSV) or a basilic vein (BaV) interposition for a high-flow access (HFA, >2 L/min) during a 3.5-year time period were included. Serial access flow, percentage of freedom from recurrent high flow and complications were determined. Results A total of 19 HFA patients were studied (11 males, age 55 ± 3 years). All AVFs were brachial artery based (brachiocephalic, n = 14; brachiobasilic, n = 5). RUDI immediately reduced access flow by almost 2 L/min (3,080 ± 200 to 1,170 ± 160 mL/min (p = 0.001)). Access flows at 1, 6 and 12 months were 1,150 ± 160, 1,460 ± 200 and 1,580 ± 260 mL/min, respectively. Postoperative complications included insufficient flow reduction (n = 1, BaV) and occlusion requiring revision (n = 1, GSV). Recurrent HFA occurred three times (n = 2 BaV, n = 1 GSV). Access flows were significantly (p<0.05) higher in the BaV group compared to the GSV group. Conclusions RUDI effectively reduces access flow in a brachial artery-based high-flow HD vascular access. A flow-reducing effect is sustained at 1-year follow-up in most patients. GSV is preferred as an interposition graft compared to a BaV.
Brachiocephalic and Basilic Fistula
Authors: Baktiroglu Selcuk , Yanar Fatih , Ozturk Alper 
Published: March 2015

Introduction Brescia-Cimino radiocephalic arteriovenous fistula (AVF) remains the first choice vascular access procedure for patients in need of long-term hemodialysis. Brachiocephalic fistulas are considered as a secondary option in almost all published guidelines. Recently in many reports, elbow and upper arm fistulas are recommended to be used as primary fistulas especially in elderly, diabetic, hypertensive patients. Elbow fistulas (brachiocephalic and brachiobasilic) should only rarely be constructed as primary fistulas. Forearm AVFs should be tried first to give a chance to the patient, because it is not the maturation-patency rates per se but also lowering the complication rates and saving the vessels for future use are equally important. Background The average life expectancy of patients receiving hemodialysis has increased in recent years and many patients now live longer and require secondary or tertiary procedures. Conclusions When forearm fistulas have failed, brachiocephalic AVF is usually preferred first, because of cephalic vein's appropriate anatomy for easy cannulation and ease of the operation. Operative procedure is less invasive compared to brachiobasilic AVF, therefore BC should be the procedure of choice when both veins are available.
Adoption of Web 2.0 applications in Omani academic libraries
Authors: Al Karousi Rahma , H.Jabr Naeema , Al Harrasi Nabhan , Bouazza Abdelmajid 
Published: March 2015

AbstractPurpose: The purpose of this paper is to identify the Web 2.0 applications adopted in Omani academic libraries (OAL), and to examine the role of Omani librarians in implementing Web 2.0 technologies. Besides, it aims to explore the importance of using Web 2.0 applications in academic libraries. Design/Methodology: Qualitative methods based on interviews were used for approaching the use of Web 2.0 applications in Omani Academic Libraries. 31 interviews with directors and library staff of four Omani academic libraries were conducted for the purpose of collecting data for this study.Findings: The results indicated that two out of the four libraries use Facebook, and only one library uses Twitter. Currently, the library of Sultan Qaboos University is starting to apply the YouTube channel and Instagram on its Beta website. Also, the findings of the study acknowledge Web 2.0 applications could enhance academic libraries in such fields as: giving the library an identity, promoting library information services, marketing library services, providing current awareness services, providing new acquisitions and communicating with library users and other libraries. Therefore, it was concluded that academic libraries should educate librarians and users regarding the use of Web 2.0 applications in library services, as well as formulate policy and rules for using these applications in Omani academic libraries.Practical implications: Most academic libraries in Oman have not yet adopted Web 2.0 applications to enhance their services. Therefore, this study will help library managers to understand the importance of using Web 2.0 applications in academic libraries and the roles of librarians in implementing Web 2.0 applications in their libraries. Besides, it can help other libraries to plan and integrate Web 2.0 applications in their services. Originality/value: This paper contributes to the research field by providing baseline data for studies on Web 2.0 applications in information science and could contribute to integrating these applications in academic libraries in general and in OAL specifically.
The role of the modern library in research evaluation
Authors: Purnell Philip James 
Published: March 2015

AbstractResearch evaluation has taken on new significance in recent years due to two main developments. The first is the enormity of data and metadata concerning research outputs making it impractical for large scale subjective peer evaluation of multinational outputs spanning research fields, linguistic barriers and varying geographies. The second is the advent of the digital age in which the medium of communicating, storing and analyzing these data seems to be continuously evolving challenging even the most high tech librarians to keep pace. New electronic resources have led to the development of new analytical and visualization tools from both the public and private sectors and library and information professionals are increasingly required to use state-of-the-art systems to inform decision making at various levels of university and research institutions. In this paper, we present a series of integrated analytical and information management tools that support large and medium sized research institutions in developed countries and that are systematically being rolled out across developing regions that are embracing the shift towards the knowledge based economy and in which libraries are expected to play an important part. Examples from the Gulf region will be demonstrated and compared with other regions and recommendations for development will conclude the session.