bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of serum levels of allergen-specific immunoglobulin E in different seasons and breeds in healthy horses
Authors: Wilkołek P.M. , Pomorski Z.J.H. , Szczepanik M.P. , Adamek Ł. , Pluta M. , Taszkun I. , Gołyński M. , Rozwód A. , Sitkowski W. 
Published: February 2015

AbstractThe present study was designed to asses specific IgE towards environment allergens in 42 healthy horses. Determination of this immunoglobulin in serum serve as diagnostic tools in allergic diseases to improve efficacy of the treatment and proper allergen selection to specific immunotherapy. Serum levels of allergen specific IgE were measured with equine monoclonal antibody, using 15 individual and 5 mix allergens in North European Panel. The study revealed season dependent increased levels of allergen specific IgE in normal horses. It is noteworthy that healthy horses show high percentage of positive reactions, most commonly towards to domestic mites D. farinae (80%), D. pteronyssinus (35.71%) and storage mites T. putrenscentiae (42.86%), Acarus siro (40.48%). These allergens play an important role in equine, canine and feline atopic dermatitis. We also demonstrated high IgE levels in the group of horse specific insect allergens. Tabanus sp. (35.71%), Culicoides sp. (28.57%) and Simulium sp. (26.19%) were the most frequent insect positive reaction allergens. No positive reactions in all groups of allergens were found in winter season, low and merely detectable levels of antibodies have been found relating to D. farianae and T. putrescentiae allergen. We observed elevated mould-IgE levels in horses that live in stables, while outdoor living horses showed very low levels. Amongst all positive reactions we observed only weak and moderate reactions but no strong positive reactions were found. No significant differences were observed between three breeds of horses with the exception of moulds and D. pteronyssinus allergens
Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders
Authors: Rypula K. , Kumala A. , Lis P. , Niemczuk K. , Płoneczka-Janeczko K. , Pejsak Z. 
Published: February 2015

Abstract
The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis
Chicken intestinal microbiota function with a special emphasis on the role of probiotic bacteria
Authors: Cisek A.A. , Binek M. 
Published: February 2015

AbstractBacterial colonization of the chicken gut by environmental microbes begins immediately after hatching. Composition of the intestinal microbiota is dependent on the surrounding environment, diet variation, pathological conditions, antibiotic therapy, and others. The genomes of all these intestinal microbes form a microbiome which by far outnumbers the host’s genome. As a consequence, the microbiome provides additional metabolic functions to the host, including nutrient utilization and absorption, fermentation of non-digestible dietary fiber, synthesis of some vitamins, biotransformation of bile acids, and the well-being of their chicken host. Microorganisms can also directly interact with the lining of the gastrointestinal tract, which may alter the physiology and immunological status of the bird. Since newly hatched broiler chickens demonstrate delayed commensal colonization and low bacterial diversity, the most effective and harmless method available to control the development and composition of the intestinal microbiota is a competitive exclusion treatment by applying probiotic bacteria. Additionally, recent research has shown that probiotic bacteria have a variety of beneficial effects, including counteraction of dysbiosis, promotion of gut health and homeostasis, enhancement of immune defenses and antagonization of infectious agents
Cytomorphometry of canine cutaneous histiocytoma
Authors: Paździor-Czapula K. , Otrocka-Domagała I. , Rotkiewicz T. , Gesek M. 
Published: February 2015

Abstract
A morphometric analysis of tumoral Langerhans cells and activated macrophages was conducted using canine cutaneous tumors (65 cases of canine cutaneous histiocytoma and 7 cases of pyogranuloma). The histiocytic origin of the tumor cells was confirmed using immunohistochemistry. The parameters of the morphometric analysis included cellular and nuclear size and shape and the nuclear: cytoplasmic ratio; the variability of these features was calculated separately for each tumor. The canine cutaneous histiocytoma group was divided into four stages of regression depending on the intensity of the lymphocytic infiltration. Statistical analysis revealed that the anisocytosis, anisokaryosis and cellular pleomorphism of tumoral Langerhans cells increased, while the cellular circularity and nuclear:cytoplasmic ratio decreased with tumor regression. Activated macrophages of the pyogranuloma were significantly larger, and had larger nuclei, than tumoral Langerhans cells. Furthermore, these activated macrophages showed greater anisocytosis and anisokaryosis and a lower nuclear: cytoplasmic ratio than tumoral Langerhans cells in the first stages of tumor regression. These results indicate that tumoral Langerhans cells undergo morphologic changes during the regression of canine cutaneous histiocytoma, reflecting their maturation and differentiation. Morphometry can be a useful method for distinguishing activated macrophages from tumoral Langerhans cells.
B-cell and T-cell values in peripheral blood in Polish mixed-breed rabbits with addition of blood of meet breeds
Authors: Tokarz-Deptuła B. , Niedźwiedzka-Rystwej P. , Hukowska-Szematowicz B. , Adamiak M. , Trzeciak-Ryczek A. , Deptuła W. 
Published: February 2015

Abstract
In Poland, rabbit is a highly valued animal, due to dietetic and flavour values of its meat, but above all, rabbits tend to be commonly used laboratory animals. The aim of the study was developing standards for counts of B-cells with CD19+ receptor, T-cells with CD5+ receptor, and their subpopulations, namely T-cells with CD4+, CD8+ and CD25+ receptor in the peripheral blood of mixed-breed Polish rabbits with addition of blood of meet breeds, including the assessment of the impact of four seasons of the year and animal sex on the values of the immunological parameters determined. The results showed that the counts of B- and T-cells and their subpopulations in peripheral blood remain within the following ranges: for CD19+ B-cells: 1.05 - 3.05%, for CD5+ T-cells: 34.00 - 43.07%, CD4+ T-cells: 23.52 - 33.23%, CD8+ T-cells: 12.55 - 17.30%, whereas for CD25+ T-cells: 0.72 - 2.81%. As it comes to the season of the year, it was observed that it principally affects the values of CD25+ T-cells, while in the case of rabbit sex, more changes were found in females.
Toltrazuril (Baycox®) treatment against coccidiosis caused by Eimeria sp. in Japanese quails (Coturnix coturnix japonica)
Authors: Sokół R. , Gesek M. , Raś-Noryńska M. , Michalczyk M. 
Published: February 2015

Abstract
Coccidiosis is the most predominant parasitic disease affecting Japanese quails (Coturnix coturnix japonica) in commercial farms. Coccidiosis as a subclinical infection is difficult to diagnose without parasitological examinations. Oocysts of two Eimeria species, E. bateri and E. tsunodai, were determined in the analysed quail flock. Infected birds were administered Baycox 2.5% at the dose of: group I - 7 mg toltrazuril/kg BW per day provided in drinking water (1.5 ml/0.5 l H2O) that was available 24 h for 2 days, group II - 14 mg/kg BW (3 ml/0.5 l H2O), and group III - 24.5 mg/kg BW (5 ml/0.5 l H2O); in groups II and III, the solutions were available 8 h/24 h for 2 days. After the first day of the treatment, the number of excreted oocysts (OPG - oocysts per gram) increased, a steady decrease in oocyst counts began on the second day of Baycox administration and lasted until a three-day period when no oocyst were determined in faecal samples. Regardless of the dose applied, toltrazuril (Baycox) completely eliminated E. bateri coccidia and led to a highly significant reduction in the number of E. tsunodai oocysts. The results suggest that the effectiveness of toltrazuril varies depending on coccidia species and developmental stages of the parasite. From the clinical point of view, the treatment applied significantly reduces the number of coccidia oocysts in commercial flocks of Japanese quails.
Modeling the effect of temperature on survival rate of Salmonella Enteritidis in yogurt
Authors: Szczawiński J. , Szczawińska M. E. , Łobacz A. , Jackowska-Tracz A. 
Published: February 2015

Abstract
The aim of the study was to determine the inactivation rates of Salmonella Enteritidis in commercially produced yogurt and to generate primary and secondary mathematical models to predict the behaviour of these bacteria during storage at different temperatures. The samples were inoculated with the mixture of three S. Enteritidis strains and stored at 5oC, 10oC, 15oC, 20oC and 25oC for 24 h. The number of salmonellae was determined every two hours. It was found that the number of bacteria decreased linearly with storage time in all samples. Storage temperature and pH of yogurt significantly influenced survival rate of S. Enteritidis (p < 0.05). In samples kept at 5oC the number of salmonellae decreased at the lowest rate, whereas at 25°C the reduction in number of bacteria was the most dynamic. The natural logarithm of mean inactivation rates of Salmonella calculated from primary model was fitted to two secondary models: linear and polynomial. Equations obtained from both secondary models can be applied as a tool for prediction of inactivation rate of Salmonella in yogurt stored under temperature range from 5 to 25°C; however, polynomial model gave the better fit to the experimental data.
The use of magnetic resonance imaging in diagnosing equine deep digital flexor tendinopathies – own experience
Authors: Jaskólska M. , Adamiak Z. , Holak P. , Zhalniarovich Y. , Przyborowska P. , Peczyński Z. 
Published: February 2015

Abstract
Deep digital flexor tendinopathy is a common problem in horses of different athletic disciplines. Nowadays, the use of magnetic resonance imaging is considered to be a noninvasive and superior choice for recognizing bone and soft tissue pathologies especially related to difficult to access structures within the hoof capsule.
Effect of dietary supplementation with Echinacea purpurea on vaccine efficacy against infection with Flavobacterium columnare in zebrafish (Danio rerio)
Authors: Guz L. , Puk K. , Walczak N. , Oniszczuk T. , Oniszczuk A. 
Published: February 2015

Abstract
The effect of dietary Echinacea purpurea (EP) on the response of zebrafish (Danio rerio) to a Flavobacterium columnare vaccine was investigated. Two hundred D. rerio with an average weight of 290 ± 40 g were selected and fed different levels of E. purpurea (5 g kg-1 diet - group 1, 10 g kg-1 diet - group 2, 20 g kg-1 diet - group 3, 30 g kg-1 diet - group 4, and 0 g kg-1 diet - group 5). Experimental feeding was begun 3 weeks prior to bath immunization and continued until the end of the experiment. Twenty-eight days after immunization the fish were challenged by bath immersion with F. columnare at a concentration of 1x106 CFU/ml. The relative percent survival of the experimental groups (1, 2, 3, 4 and 5) was 5.0, 6.0, 30.0, 36.0 and 5.0, respectively. In conclusion, diet supplementation with E. purpurea may effectively enhance the response of zebrafish to a F. columnare vaccine.
A 34-year retrospective study of equine viral abortion in Poland
Authors: Bażanów B.A. , Frącka A.B. , Jackulak N.A. , Staroniewicz Z.M. , Ploch S.M. 
Published: February 2015

Abstract
The purpose of the present review was a comparison of the abortions caused by EAV and EHV-1 viruses over the 34 years. A total of 452 tissues samples from aborted fetuses (347) or foals (105) stillborn or newborn that died within 72 hours were investigated. The material for the examinations came from different farms located throughout Poland. The tissue homogenates were examined by using virus isolation test in RK-13 and Vero cell lines and the cytopathic agent was confirmed as EHV-1 by the direct fluorescent antibody test or as EAV by the indirect fluorescent antibody test. The study indicated that EAV was isolated (104 cases, 23%) almost as equally often as EHV-1 (116 cases, 25.6%). Both, equid herpesvirus-associated abortion and the abortion induced by EAV were characterized by cyclicity. The percentage of EAV and EHV-1 isolation alternately reduced and increased, but the increase of isolation of one virus was accompanied by the decrease of the other. The domination of one virus over the other occurred in cycles of a few years.
Values of white and red blood cell parameters in Polish mixed breed rabbits in the annual cycle
Authors: Tokarz-Deptuła B. , Niedźwiedzka-Rystwej P. , Adamiak M. , Hukowska-Szematowicz B. , Trzeciak-Ryczek A. , Deptuła W. 
Published: February 2015

AbstractThe aim of the study was to develop reference values for such haematologic values as haemoglobin concentration, haematocrit value, volume of erythrocytes, thrombocytes, leucocytes, lymphocytes, neutrophils, basophils, eosinophils and monocytes in peripheral blood in Polish mixed-breed rabbits, considering the impact of the season (spring, summer, autumn, winter), and sex of the animals. The results reveal, that the season of the year has a small impact in Polish mixed-breed rabbits, and it principally referred to spring and summer. Finally it was shown, that sex has average impact on the studied values of haematological parameters.
Circulating immune complexes and markers of systemic inflammation in RAO-affected horses
Authors: Niedźwiedź A. , Jaworski Z. , Kubiak K. 
Published: February 2015

Abstract
The aim of this study was to investigate the levels of circulating immune complexes (CICs) and concentration of haptoglobin, fibrinogen and C-reactive protein in the serum of horses with recurrent airway obstruction and healthy controls. The study was conducted on a group of 14 adult Polish Konik horses, kept in uniform environmental and living conditions. Horses were divided into two groups: 7 horses were not affected by any respiratory problem (control group) and 7 horses had a history of recurrent airway obstruction (RAO) (study group). A clinical and laboratory evaluation, endoscopic examination and bronchoalveolar lavage (BAL) were performed in all horses. Levels of circulating immune complexes were significantly (p=0.0057) increased in heaves-affected horses compared to healthy controls (median [25th - 75th percentiles]) (3.96 [3.96 - 4.43] vs. 7.46 [5.13 - 11.9]). No significant difference was observed in the levels of the examined acute phase proteins between the groups. Moreover, all results were within the reference range established for horses. The results of this study indicate that heaves in horses is associated with the formation and high level of CICs. Haptoglobin, fibrinogen and C-reactive protein failed as markers of early stage systemic inflammation in the course of RAO.
Small interfering RNA treatment can inhibit Cyprinid herpesvirus 3 associated cell death in vitro
Authors: Adamek M. , Rauch G. , Brogden G. , Steinhagen D. 
Published: February 2015

Abstract
A Cyprinid herpesvirus 3 infection of carp induces a disease which causes substantial losses in carp culture. Here we present the use of a possible strategy for the management of the virus infection RNA interference based on small interfering RNAs. As a result of in vitro studies, we found that a mixture of short interfering RNAs specific for viral DNA enzyme synthesis and capsid proteins of the CyHV-3 can be a potential inhibitor of virus replication in fibroblastic cells. This gives the basis for the development of a combinatorial RNA interference strategy to treat CyHV-3 infections.
Influenza virus proteins as factors involved in interspecies transmission
Authors: Góra I.M. , Rożek W. , Żmudziński J.F. 
Published: February 2015

AbstractInfluenza A viruses cause recurrent epidemics and global pandemics. One of the unique features of influenza virus is the ability to overcome interspecies barrier. Reassortment of viral genes and the accumulation of mutations contribute to the emergence of new influenza virus variants. The replication of influenza A virus in a specific host depends on many factors e.g. activity of viral proteins, host response system and environmental conditions. In this review the role of viral proteins as a condition for crossing the species barriers is discussed.
In the trap: detectability of fixed hair trap DNA methods in grizzly bear population monitoring
Authors: Rovang Sarah , Nielsen Scott E. , Stenhouse Gordon 
Published: February 2015

A significant challenge to monitoring wildlife that are secretive, wide ranging, and at low densities is the need to achieve adequate detection rates. Knowledge of spatial patterns in occupancy and the spatial and/or temporal patterns in detectability allows for stratification of traps and improved detection rates. This study investigated how local variation in habitats affected the detectability of grizzly bears in west—central Alberta when monitored with a fixed DNA hair snag design. Bear hair samples were collected in 2011 at 60 sites across 1500 km2 over six sessions, each 14 days in length, between June and August. Microsatellite analysis of hair samples revealed grizzly bear detections at 25 of 60 sites and 21 individual bears. We investigated occupancy and detectability of grizzly bears at the patch and landscape scales using detection histories and program PRESENCE. At the patch scale, grizzly bear detection was highest when sites were placed near streams with clover and in intermediate levels of forest crown closure. At the landscape scale, probability of detection increased near streams and oil and gas wellsites, especially when food resources and wellsite density in the surrounding area was low. Our results highlight the importance of considering local food resources and habitat conditions during placement of fixed DNA hair snag sites. Detectability was not found to vary over time, suggesting that sampling in west—central Alberta can occur at any time between June and August.
Hyponatremia and poor cognitive outcome following pediatric brain tumor surgery
Authors: Williams Cydni N. , Riva-Cambrin Jay , Presson Angela P. , Bratton Susan L. 
Published: February 2015

OBJECT
Pediatric intracranial neoplasms are common and cause substantial neurological morbidity. Postoperative hyponatremia is also common and may exacerbate neurological injury. The authors performed an exploratory analysis to evaluate an exposure-response relationship between hyponatremia severity and cognitive function at discharge.

METHODS
A retrospective cohort of patients 0–19 years old who underwent a first intracranial neoplasm surgery at a pediatric tertiary care hospital was reviewed. Outcome was assessed by Pediatric Cerebral Performance Category (PCPC) score of 1–6 at hospital discharge. Poor outcome was defined as PCPC score 3–6, corresponding to moderate or worse disability.

RESULTS
Of 319 total children, 80 (25%) had poor outcomes. One hundred thirty-seven children (43%) had serum sodium concentrations ≤ 131–135 mEq/L and 39 (12%) had serum sodium concentrations ≤ 130 mEq/L. Lower nadir sodium concentration and longer duration of hyponatremia were significantly associated with worsening PCPC score (p < 0.001). Rapid sodium decreases and more hyponatremic episodes were also significantly associated with worsening PCPC score (p < 0.001). After adjustment for patient factors, tumor characteristics, and measures of sodium disruption, multivariable analysis revealed noncortical tumor locations and lower nadir sodium concentration (adjusted odds ratio 0.86, 95% confidence interval 0.78–0.95) were important independent risk factors for poor cognitive outcome.

CONCLUSIONS
Neurocognitive disability and hyponatremia are common in children undergoing surgery for intracranial neoplasms. This study found a significant association between severity of hyponatremia and worsened cognitive outcome, with an apparent exposure-response relationship. These data support the need for careful postoperative monitoring of serum sodium. Further research is needed to determine if prevention and treatment of hyponatremia can improve outcomes in these children.
Demolitions and Amendments: Coping with Cultural Recognition and Its Denial in Southern Israel
Authors: McKee Emily 
Published: February 2015

Abstract
This article examines how social preferences, in the form of cultural politics, become concretised in land laws. In Israel, Bedouin Arabs in unrecognised villages and Jewish farmers of individual farmsteads each faced governmental eviction orders and responded by seeking recognition of their land-use practices as legal. However, whereas Jewish farmers successfully mobilised place-based identities to gain legalisation, Bedouin Arabs' dwelling practices were not recognised as the legitimate basis for land claims, and their attempts to assert place-based identities have been denied. Instead, Bedouin Arabs faced pressures of 'de-cultural accommodation' and continued evictions. Ethnographic comparison of these two cases of 'illegal' settlement demonstrates how cultural identities - as former nomads or pioneer farmers - matter for land claims.
Stationäre nuklearmedizinische Therapie 2010 bis 2012 in Deutschland
Authors: Buck A. , Reiners C. , Lorenz R. 
Published: February 2015

ZusammenfassungDas Ziel dieser Analyse war es, deutschlandweite Daten über den Status und die Entwicklung der stationären nuklearmedizinischen Therapie zu ermitteln. Als Datenquelle wurden die gesetzlichen Qualitätsberichte der Krankenhäuser verwendet. Methodik: Die vom Gemeinsamen Bundesausschuss (G-BA) aus den maschinenverwertbaren XML-Daten der Qualitätsberichte erstellten Referenzberichte aller Kliniken Deutschlands mit nuklearmedizinischer Therapiestation wurden für die Jahre 2010 und 2012 analysiert. Für die Beurteilung der längerfristigen Entwicklung dienten Ergebnisse aus eigenen Voruntersuchungen der strukturierten Qualitätsberichte für die Jahre 2004, 2006 und 2008. Zur Ermittlung der deutschlandweiten Häufigkeit von Schilddrüsenoperationen und der Radioiodtherapie wurden öffentliche Datenbanken des Instituts für das Entgeltsystem im Krankenhaus (InEK) für die Jahre 2004 bis 2012 ausgewertet. Ergebnisse: Die Gesamtzahl der stationären nuklearmedizinischen Behandlungsfälle hat von 2010 mit 50 363 Patienten bis 2012 auf 47 314 Patienten gering abgenommen. Im längeren Verlauf von 2004 bis 2012 findet sich ein deutlicher Rückgang der Fälle von 17,5%. Der Rückgang ist vor allem durch eine Abnahme an Erkrankungen mit Hyperthyreose (ICD-Code E05) bedingt. Seit 2009 ist auch die Anzahl der Schilddrüsenoperationen rückläufig. Ein moderater Anstieg der Fallzahlen von 23,7% zeigt sich für die Diagnose Schilddrüsenkarzinom (ICD-Code C73) von 2004 bis 2012. Schlussfolgerungen: Vermutlich führt die verbesserte Iodversorgung in Deutschland zu einem Rückgang an stationären Patienten mit Hyperthyreose in der Nuklearmedizin und in der Folge zu einer Abnahme sowohl der Anzahl an Radioiodtherapien als auch der Schilddrüsenoperationen in der Chirurgie. Im Gegensatz hierzu ist die Anzahl der Patienten mit der Diagnose Schilddrüsenkarzinom auf nuklearmedizinischen Therapiestationen moderat ansteigend, dies korreliert mit der weltweit zu beobachtenden Steigerung der Inzidenz des Schilddrüsenkarzinoms.