bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic revelations: investigating rotavirus a presence in wild ruminants and its zoonotic potential
Authors: Šenica Petra , Žele Vengušt Diana , Vengušt Gorazd , Kuhar Urška 
Published: August 2024

IntroductionRotaviruses A (RVA) are a major cause of acute viral gastroenteritis in humans worldwide and are responsible for about two million hospitalizations per year. They can also infect other mammals such as pigs, calves, goats, lambs, and horses, in which they are also considered a major cause of viral diarrhea. While RVA is well studied in humans and domestic animals, its occurrence in wild ruminants is not well known. The RVA genome is a double-stranded RNA consisting of 11 segments, and genotyping is based on the VP7 (G) and VP4 (P) segments. Currently, there are 42G genotypes and 58P genotypes. RVA has a high mutation rate, and some combinations of G and P genotypes can infect different animal species, leading to speculation about the potential for zoonotic transmission.Materials and methodsA total of 432 fecal samples were collected from roe deer, red deer, chamois, mouflon and Alpine ibex in Slovenia between 2017 and 2021. To investigate the presence of RVA in wild ruminants, real-time RT-PCR was used. Positive samples were subjected to next generation sequencing (NGS) using RIP-seq method.Results and discussionIn total, 7 samples were RVA positive. Complete genomes were determined and phylogenetically analyzed for all 7 RVAs. Four different genotype constellations were present in 7 positive RVA animals: G8-P[14]-I2- R2-C2-M2-A3-N2-T6-E2-H3, G6-P [14]-I2-R2-C2-M2-A11-N2-T6-E2-H3, G10-P [15]-I2-R2-C2-M2-A3-N2-T6-E2-H3 and G10-P [15]-I2-R2-C2-M2-A11- N2-T6-E2-H3. Genotypes G6P[14] and G10P[15] were found in both roe deer and red deer, representing the first confirmed occurrence of RVA in red deer. In addition, genotype G8P[14] was found in chamois, representing the first known case of positive RVA in this species. Some of these genotypes have also been found in humans, indicating the potential for zoonotic transmission.
A Retrospective Study of Genetic Characterization in Suspected Visceral Leishmaniasis Cases in Greece, 2005 to 2020
Authors: Evangelidou Maria , Makka Sofia , Papadogiannaki Ioanna , Koutantou Myrto , Tegos Nikolaos , Mpimpa Anastasia , Patsoula Eleni , Angelakis Emmanouil 
Published: August 2024

Leishmania infantum is considered the predominant Leishmania species responsible for visceral leishmaniasis (VL) in Greece but limited molecular-typing-based studies have been performed so far. We retrospectively analyzed data and serum samples collected from 3661 individuals suspected for VL in a sixteen-year period, from 2005 to 2020, to study the seasonality and demographic characteristics of VL cases and to define the L. infantum genotypes circulating in the country. Serum samples were tested with immunofluorescence assay and/or molecular assay. qPCR Leishmania-positive samples were subjected to genotypic analysis based on polymorphisms in 12 microsatellite regions of the internal transcribed spacers (ITSs) 1 and 2. We diagnosed 219 definite (6%, sample with a positive molecular assay and/or antibody titer ≥ 1:400) and 230 probable (6.3%, sample with antibody titer between 1:100 and 1:200) VL cases. Data analysis revealed that amongst VL-definite cases, the age group (≥65) constitutes the most affected factor, since 36.9% of the VL cases belonged to this age group. Amongst the VL definite cases, the most frequently reported symptoms were fever (83%), splenomegaly (49%), and hepatomegaly (40%), but this was not the case for immunocompromised patients that developed non-typical symptoms of leishmaniasis. Although no statistically significant differences in the overall seasonality of VL cases were observed, February and June showed a significantly higher proportion of VL cases compared to August and December. Genotyping of ITS1 and ITS2 regions revealed that all VL cases belong to ITS type A of L. infantum. Our study provides epidemiological information on VL and demonstrates for the first time, providing genotypic data, the circulation of ITS type A L. infantum in Greece.
Green Synthesis of Yttrium Derivatives Nanoparticles Using Pine Needle Leaf Extract: Characterization, Docking, Antibacterial, and Antioxidant Potencies
Authors: Darwich Nourhane A. , Mezher Malak , Abdallah Alaa M. , El-Sayed Ahmed F. , El Hajj Rana , Hamdalla Taymour A. , Khalil Mahmoud I. 
Published: August 2024

Green nanoparticles are synthesized using environmentally friendly methods, and natural materials hold significant importance. This makes the process environmentally sustainable and reduces the production of harmful waste by-products. Green nanoparticles exhibit reduced toxicity which is crucial for biomedical applications. The current study suggested that yttrium nanoparticles (YNPs) should be synthesized, characterized, and evaluated for their diverse biological applications due to the rise in antibacterial resistance. The YNPs were prepared using a pine needle leaf extract (PNLE). The structural and morphological features have been investigated using X-ray diffraction (XRD), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), X-ray photoelectron spectroscopy (XPS), photoluminescence (PL), Fourier transform infrared spectroscopy (FTIR), ultraviolet–visible spectroscopy (UV–vis), and vibrating sample magnetometry (VSM). The XRD pattern demonstrated the presence of yttrium oxide and yttrium nitrate phases. The crystallite size and particle size of the synthesized YNPs measured 1.696 nm and 24.55 nm, respectively. The XPS peaks showed two components with binding energies at 530.940 eV and 532.18 eV due to the bond between O–Y and OH–Y, respectively. Additionally, the ferromagnetic nature of the YNPs was confirmed by VSM analysis. The YNPs were tested for antibacterial activity on six uropathogenic bacteria (S. aureus, S. haemolyticus, E. faecalis, E. coli, K. pneumonia, and P. aeruginosa) using the microdilution assays, to find the minimum inhibitory concentration (MIC) as well as the minimum bactericidal concentration (MBC), the agar well diffusion assay, and antibiofilm screening assays, where they showed bacteriostatic action against all isolates (0.5–1 mg/mL MIC) and significant inhibition of biofilm formation (80% inhibition rate). The antioxidant capacity assessed by 1,1, diphenyl-2-picrylhydrazyl (DPPH) radical scavenging revealed 50% DPPH scavenging. Moreover, docking studies exhibited that YNPs inhibit crucial bacterial enzymes, including DNA gyrase, penicillin-binding proteins, carbapenemase, LasR-binding protein, and dihydropteroate synthase. These findings may explain the mechanisms responsible for the observed antibacterial effects of YNPs. Overall, these findings underscore YNPs as promising candidates for antioxidant and antibacterial applications.
Patient Preferences and Priorities for the Design of an Acute Kidney Injury Prevention Trial
Authors: Elliott Meghan J. , Fiest Kirsten M. , Love Shannan , Birdsell Dale , Loth Maureena , Dumka Heather , Rana Benny , Shommu Nusrat , Benterud Eleanor , Gil Sarah , Acharya Dilaram , Harrison Tyrone G. , Pannu Neesh , James Matthew T. 
Published: August 2024

Key Points

For AKI prevention trial recruitment, patients prioritized technology enabled prescreening and involvement of family members in the consent process.For trial intervention delivery, participants prioritized measures to facilitate ease of trial intervention administration and return visits.For AKI prevention trial outcomes, patient participants identified effects on kidney-related and other clinical outcomes as top priorities.

Background
High-quality clinical trials are needed to establish the efficacy and safety of novel therapies for AKI prevention. In this consensus workshop, we identified patient and caregiver priorities for recruitment, intervention delivery, and outcomes of a clinical trial of cilastatin to prevent nephrotoxic AKI.

Methods
We included adults with lived experience of AKI, CKD, or risk factors of AKI (e.g., critical care hospitalization) and their caregivers. Using a modified nominal group technique approach, we conducted a series of hybrid in-person/virtual discussions covering three clinical trial topic areas: (1) consent and recruitment, (2) intervention delivery, and (3) trial outcomes. Participants voted on their top preferences in each topic area, and discussion transcripts were analyzed inductively using conventional content analysis.

Results
Thirteen individuals (11 patients, two caregivers) participated in the workshop. For consent and recruitment, participants prioritized technology enabled prescreening and involvement of family members in the consent process. For intervention delivery, participants prioritized measures to facilitate ease of intervention administration and return visits. For trial outcomes, participants identified kidney-related and other clinical outcomes (e.g., AKI, CKD, cardiovascular events) as top priorities. Analysis of transcripts provided insight into care team and family involvement in trial-related decisions, implications of allocation to a placebo arm, and impact of participants' experiences of AKI and critical illness.

Conclusions
Findings from our workshop will directly inform development of a clinical trial protocol of cilastatin for nephrotoxic AKI prevention and can assist others in patient-centered approaches to AKI trial design.
ALKOHOLUN UZUN MÜDDƏT TƏSİRİ NƏTİCƏSİNDƏ AĞ SİÇOVULLARIN BAŞ BEYNİ STRUKTURLARINDA MONOAMİNLƏRİN MİQDARİ DƏYİŞİKLİKLƏRİNİN KORREKSİYASI
Authors: Мамедгасанов Т.С. 
Published: August 2024

Представлены результаты исследования, проведенного с целью фармакологического изучения возможности корригирующего действия мексидола и пирацетама на изменение количества моноаминов в разных структурах головного мозга белых крыс при длительном воздействии алкоголя. Анализ результатов проведенных исследований показал, что изменение количества моноаминов в разных структурах головного мозга белых крыс (гипоталамус, стриатум, лобная кора) при длительном применении алкоголя приближается к исходному уровню под влиянием мексидола и пирацетама. Более быстрая нормализация содержания моноаминов наблюдалась при совместном применении мексидола и пирацетама.
Məqalədə xronik alkohol qəbulu fonunda ağ siçovullarda baş beyninin müxtəlif strukturlarında monoaminlərin miqdarında baş verən dəyişikliklərə meksidol və pirasetamın korrektor təsirinin farmakoloji tədqiqinə həsr edilmiş tədqiqat işi haqqında məlumat verilmişdir. Tədqiqatın nəticələrinin təhlili göstərmişdir ki, meksidol və pirasetamın təyini fonunda xronik alkohol qəbulu ilə bağlı ağ siçovulların baş beyninin müxtəlif strukturlarında (hipotalamus, striatum, frontal qabıq) monoaminlərin miqdar dəyişikliklərini əhəmiyyətli dərəcədə aradan qaldırır. Ən yaxşı effekt meksidol və pirasetamın birlikdə istifadəsi zamanı müşahidə edilir.
The article provides information about a pharmacological research study dedicated to changes in the levels of monoamines in the white matter of the brain during chronic alcohol consumption. This study focused on the corrective effects of “Mexidol” and “Piracetam”. The analysis of the research findings showed that the changes in the levels of monoamines in different brain structures of white rats (hypothalamus, striatum, frontal cortex) during prolonged alcohol consumption approach the initial levels under the influence of “Mexidol” and “Piracetam”. A faster normalization of monoamine content was observed with the simultaneous administration of “Mexidol” and “Piracetam”.
CANDLE-ПОДОБНЫЙ СИНДРОМ: КЛИНИЧЕСКОЕ НАБЛЮДЕНИЕ
Authors: Quliyeva N.M. 
Published: August 2024

Məqalədə nadir patologiyalardan biri olan CANDLE sindromuna (Chronic Atypical Neutrophilic Dermatosis with Lipodystrophy and Elevated temperature) görə müalicə almış 1 yaş 5 aylıq xəstə qız haqqında məlumat verilir. Xəstə kompleks klinik, immunoloji laborator, instrumental müayinələrdən keçirilmiş, genetik analiz olaraq PSMB4, PSMB8, PSMB9 və PSMA3 genləri araşdırılsa da, bu genlər aşkar olunmayıb, lakin bütün klinik əlamətlərə və xəstəliyin gedişinə əsasən Candle sindromuna bənzədiyinə görə, xəstəyə CANDLE-yə bənzər sindrom diaqnozu qoyulmuşdur. Məqalədə qeyri-spesifik immun mexanizm vasitəsilə, bəzi hallarda isə spesifik immun tənzimin pozulması nəticəsində yaranan və autosom-ressesiv yolla ötürülən CANDLE sindromu haqqında qısa məlumatla yanaşı, müəllifin praktikada müşahidə etdiyi klinik nümunənin şərhi verilmişdir. Bu xəstəliyin tədqiqi klinik əlamətlərinin tipik xüsusiyyətlərinə, diaqnostika və müalicəsinin çətinliyinə görə böyük praktik əhəmiyyət kəsb edir.В статье представлены сведения о девочке 1 года 5 месяцев с одной из редких патологий – CANDLE-подобным синдромом (хронический атипичный нейтрофильный дерматоз с липодистрофией и повышенной температурой). Больному проведено комплексное клинико-иммунологическое лабораторное и инструментальное обследование, в качестве генетического анализа исследованы гены PSMB4, PSMB8, PSMB9 и PSMA3, но эти гены не обнаружены, однако учитывая, что все клинические признаки и течение заболевания сходны CANDLE-синдромом, у больной установлен диагноз CANDLE-подобный синдром. В статье наряду с краткими сведениями о синдроме CANDLE, который обусловлен неспецифическим иммунным механизмом, а при некоторых случаях как следствие специфического нарушения иммунной регуляции и передается по аутосомно-рецессивному пути, приведен пример, наблюдаемый в практике автора. Изучение этого заболевания имеет большое практическое значение в связи с типичными особенностями его клинической симптоматики, сложностью диагностики и лечения.The article provides information about a 1-year and 5-month-old girl with one of the rare pathologies – CANDLE-like syndrome (Chronic Atypical Neutrophilic Dermatosis with Lipodystrophy and Elevated Temperature). The patient underwent a comprehensive clinical-immunological laboratory and instrumental examination. Genetic analysis of the PSMB4, PSMB8, PSMB9, and PSMA3 genes was conducted, but these genes were not detected. However, considering that all clinical signs and the course of the disease are similar to CANDLE syndrome, the patient was diagnosed with CANDLE-like syndrome. The article briefly outlines information about CANDLE syndrome, which is caused by a non-specific immune mechanism and, in some cases, as a result of a specific immune regulation disorder, transmitted in an autosomal recessive manner. Alongside this, the article presents a case observed in the author's practice. The study of this disease is of great practical importance due to the typical features of its clinical symptoms, the complexity of diagnosis, and treatment.
КЛИНИКО-АНАМНЕСТИЧЕСКАЯ ХАРАКТЕРИСТИКА ОСТРОЙ КРАПИВНИЦЫ У ДЕТЕЙ И УРОВЕНЬ СЕКРЕТОРНОГО IgA В ПОЛОСТИ РТА БОЛЬНЫХ
Authors: Şixəmmədova A.C. 
Published: August 2024

Məqalədə kəskin övrə diaqnozu qoyulmuş uşaqların klinik anamnestik xüsusiyyətlərinin öyrənilməsi və bu xəstəlik zamanı ağız boşluğunda sekretor IgA səviyyəsinin araşdırılması məqsədilə aparılmış tədqiqat işi haqqında məlumat verilir. Əsas tədqiqat qrupuna kəskin övrə diaqnozu qoyulmuş 80 uşaq, kontrol qrupa isə 20 praktik sağlam uşaq daxil edilmişdir . Tədqiqata cəlb edilmiş xəstə və sağlam uşaqların ağız suyundakı SIgA (sekretor IgA) – immunofermet analizi ( İFA) metodu ilə Medispec-6000 aparatında təyin edilmişdir. Tədqiqat göstərmişdir ki, kəskin övrə xəstəliyi diaqnozu ilə tədqiqata cəlb edilmiş uşaqlar arasında yaşı 3-dən 6-ya qədər olanlar üstünlük təşkil edirlər – 42 nəfər (52,5%). Etioloji amillər arasında qida allergenlərinin rolu daha böyükdür. Xəstələrin ağız boşluğunda SIgA səviyyəsi kontrol qrupdakına nisbətən 1,3 dəfə aşağı olmuşdur.
В статье представлены данные по изучению клинико-анамнестической характеристики детей с диагнозом острая крапивница, а также исследованию уровня секреторного IgA в полости рта при этом заболевании. В основную группу исследования вошли 80 детей с диагнозом острая крапивница, в группу контроля — 20 практически здоровых детей. SIgA (секреторный IgA) в слюне больных и здоровых детей, участвующих в исследовании, определяли методом иммуноферментного анализа (ИФА) на аппарате Medispec-6000. Исследование показало, что среди включенных в исследование детей с диагнозом острая крапивница преобладали пациенты в возрасте от 3 до 6 лет - 42 ребенка (52,5%). Среди этиологических факторов преобладали пищевые аллергены. Уровень SIgA в полости рта больных был в 1,3 раза ниже, чем в контрольной группе.
The article presents data from a study on the clinical and anamnestic characteristics of children with acute urticaria, as well as the examination of secretory IgA levels in the oral cavity. The main study group comprised 80 children diagnosed with acute urticaria, while the control group consisted of 20 practically healthy children. Secretory IgA (SIgA) in the saliva of both sick and healthy children participating in the study was determined using enzyme-linked immunosorbent assay (ELISA) with the Medispec-6000 apparatus. The study revealed that among the children with acute urticaria included in the study, those aged 3 to 6 years predominated, comprising 42 children (52.5%). Food allergens were predominant among the etiological factors. The level of SIgA in the oral cavity of patients was 1.3 times lower than that in the control group.
Consumer Protection Againts Innappropriate Online Goods Purchases Reviewed From The Civil Code and Consumer Protection Law
Authors: Turnip Johannes Mangapul , Amalia Apri 
Published: August 2024

Online buying and selling transactions are carried out by some Indonesian people through e-commerce platform whice are a technological development. Online purchasing transactions are carried out to save time and make things easier for buyers. However, there is a phenomenon in online purchases in the form of consumer complaints regarding dissatisfaction with the goods purchased which do not match the information provided by the seller. The identification of the research problem is how consume law is protected in the civil code and consumer protection law and the responsibility of business actors for consumer losses. The aim of the research is to determine consumer legal protection and the responsibilities of business actors towards consumers. The research uses normative juridical research, a statutory regulation approach and is analysed qualitatively. Consumer protection is regulated in the civil code and consumer protection law number 8 of 1999 which regulates the protection and replacement of goods if the goods received by the consumer do not conform to what was agreed in the sale and purchase transaction. The responbility of business actors for consumer losses is to provide compensation for demage, replacement of goods and/or refunds
ANALISIS PERILAKU GERAK ELEKTRON ATOM HIDROGEN MELALAUI SIMULASI KOMPUTER
Authors: Tawil Muh 
Published: August 2024

Simulasi komputer persamaaan Scrodinger pada atom hidrogen bermanfaat untuk memberi gambaran perilaku gerakan partikel elektron pada berbagai bilangan kuantum pada atom hidurogen yang tidak dapat diamati secara eksperimen. Tujuan dari penelitian ini untuk mengkaji bagaimana karakteristik gerak elektron di dalam atom hidrogen. Hasil peneltian diperoleh gambaran karakteristik posisi keberadaan elektron di dalam atom hidrogen dengan memperhitungkan ketiga variabel, yakni variabel posisi, gerak orbital dan gerak azimut. Implikasi dari hasil kajian ini adalah menujukkan konsep-konsep secara kongkret tentang karakteristik gerak elektron di dalam atom hidrogen, yang selama ini sangat sulit untuk dipahami. Kata kunci : gerak elektron, atom hidrogen, posisi, orbital elektron
Chemical and disinfectant properties of a chlorine-containing chemical from underground mineralized water
Authors: Соколов Л.И. , Силинский В.А. 
Published: August 2024

Изучено влияние хлорсодержащего реагента, полученного электролизом из подземных минерализованных вод, на химические и бактериологические показатели сточных вод. Для проведения эксперимента по обеззараживанию проб сточной воды был получен хлорсодержащий реагент путем предварительного воздействия ультразвука на минерализованную подземную воду (с содержанием хлоридов 20 г/л) и последующего электролиза этой воды на экспериментальной установке, имеющей титановые электроды. Установлена высокая дезинфицирующая способность реагента на завершающей стадии очистки сточных вод. Подтверждены слабые дезинфицирующие свойства хлораминов в сравнении с хлорноватистой кислотой и гипохлоритом натрия.
The effect of a chlorine-containing chemical prepared by electrolysis from underground mineralized water on the chemical and bacteriological parameters of wastewater was studied. To conduct an experiment on the disinfection of wastewater samples, a chlorine-containing chemical was prepared by preliminary ultrasound exposure on mineralized groundwater (with a chloride content of 20 g/l) and subsequent electrolysis of this water in an experimental unit with titanium electrodes. The high disinfecting effect of the chemical at the final stage of wastewater treatment has been established. The weak disinfecting properties of chloramines in comparison with hypochlorous acid and sodium hypochlorite have been confirmed.
The Effect Of Spraying Enzylaw Liquid Organic Fertilizer (Poc) On Crop Production Allium fistulosum L.
Authors: Pantow Wilhelm 
Published: August 2024

This research aims to determine the effect of enzylaw liquid organic fertilizer (POC) on the production of spring onions (Allium fistulosum L.). The research method used was the Completely Randomized Design (CRD) method because the experimental media/materials were homogeneous or uniform. Consists of 6 treatments, as follows; A: Control, B: 1 ml enzyme/1 liter of water, C: 2 ml enzyme/1 liter of water, D: 3 ml enzyme/1 liter of water, E: 4 ml enzyme/1 liter water, F: 5 ml enzyme/1 liter of water. This research activity was carried out from January to March 2023 in Paslaten Village, East Tomohon District. The results of the research showed that the use of Enzylaw liquid organic fertilizer (POC) had an effect on the growth of leek plants. Giving POC enzylaw at a dose of 5 ml of enzyme/1 liter of water produces on average: (1) The highest number of stems (134 stems), (2) Produces the highest average number of leaves, namely 375 leaves, and (3) Produces an average of the highest average wet weight, namely 4823 g.
Keywords: liquid organic fertilizer (POC), Enzylaw, Allium fistulosum L.
Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh pupuk organik cair (POC) enzylaw terhadap produksi bawang daun (Allium fistulosum L.). Metode penelitian yang digunakan yaitu metode Rancangan Acak Lengkap (RAL), karena media/bahan percobaan homogen atau seragam. Terdiri atas 6 perlakuan, sebagai berikut ;A : Kontrol, B : 1 ml enzylaw/1 liter air, C : 2 ml enzylaw/1 liter air, D : 3 ml enzylaw/1 liter air, E : 4 ml enzylaw/1 liter air , F : 5 ml enzylaw/1 liter air. Waktu kegiatan penelitian ini dilaksanakan pada bulan Januari sampai Maret 2023 di Kelurahan Paslaten Kecamatan Tomohon Timur. Hasil penelitian menunjukkan bahwa penggunaan pupuk organik cair (POC) enzylaw berpengaruh terhadap petumbuhan tanaman bawang daun. Pemberian POC enzylaw dengan dosis 5 ml enzylaw/1 liter air menghasilkan rata-rata: (1) Jumlah batang terbanyak (134 batang), (2) Menghasilkan rata-rata jumlah daun terbanyak, yaitu 375 helai, dan (3) Menghasilkan rata-rata berat basah tertinggi, yaitu 4823 g.

Kata kunci: POC, Enzylaw, Allium fistulosum L.