bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme, using Illumina sequencing
Authors: Trapnell Dorset W. , Beasley Rochelle R. , Lance Stacey L. , Field Ashley R. , Jones Kenneth L. 
Published: June 2015

Premise of the study:Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon.Methods and Results:Nineteen microsatellite loci were identified from an Illumina paired‐end shotgun library of D. calamiforme. Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ∼80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380.Conclusions:These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.
Le métier de photographe
Authors: Vauclare Claude , Debeauvais Rémi 
Published: June 2015

Évalués à environ 25 000, les photographes professionnels font face, depuis une quinzaine d’années, à des changements importants liés à la diffusion des nouvelles technologies numériques, qui ont redéfini les pratiques de toute une profession. La mutation des conditions de création, de production et de diffusion de la photographie s’est traduite par une dérégulation du marché de la photographie et par une fragilisation du cadre juridique qui garantissait auparavant les revenus des photographes. L’étude est fondée sur une enquête menée auprès d’un échantillon de 3 000 photographes et sur de nombreux entretiens avec des professionnels. Elle présente les caractéristiques sociodémographiques de cette population professionnelle en expansion depuis quinze ans (+ 37 %) qui se renouvelle en se féminisant. Elle décrit la diversité des statuts professionnels, juridiques et fiscaux qui encadrent l’exercice du métier de photographe et les conditions de l’activité : positionnement sur le marché, revenus nets d’activité, évolution des techniques photographiques. Enfin, l’étude décrit les représentations que les professionnels ont de leur métier et de son avenir : adaptation nécessaire aux innovations technologiques, concurrence de la photographie en amateur par exemple, et les attentes qu’ils ont exprimées, notamment en termes de simplification des régimes sociaux et fiscaux, d’accès à la formation professionnelle continue et de protection du droit du photographe auteur.
L’apport de la pluralité des méthodes dans la prévention du risque psycho-socio-organisationnel
Authors: Machado Tony , Desrumaux Pascale , Lancry Alain 
Published: June 2015

Le diagnostic et la prévention du risque psycho-socio-organisationnel (RPSO) nécessite l’utilisation de méthodes complémentaires (examen des indicateurs de fonctionnement et de santé, entretiens, analyse de l’activité, adaptation d’un questionnaire) permettant de prendre en compte différentes exigences et ressources impactant la santé des travailleurs. L’intervention menée dans le secteur de l’hôtellerie-restauration (N = 230) a mobilisé ces différentes méthodes interrogeant des déterminants organisationnels propres à l’entreprise. Les entretiens et les analyses de l’activité ont permis d’identifier des exigences (exposition aux comportements antisociaux, risque perçu d’exposition à un accident du travail, exigences physiques et environnementales) et des ressources (communication, accompagnement au changement) complémentaires à celles des modèles de référence de Karasek et Siegrist (demande, latitude, soutien, récompense). Les résultats indiquent que la prise en compte des exigences et ressources spécifiques permet de mieux expliquer les atteintes sur la santé, évaluée sous les dimensions de stress, d’épuisement physique et émotionnel. Par ailleurs, conformément au modèle JDR, un rôle modérateur des ressources sur le lien existant entre exigences et santé a été observé. Lors de l’évaluation du RPSO, il semble donc nécessaire d’articuler l’intervention autour d’une méthodologie combinatoire afin d’être au plus près des réalités de terrain vécues par les agents et construire un plan d’actions de prévention efficace.
Riz, terre et eau du Lango : la lente émergence d’un territoire hydraulique ougandais
Authors: Torretti Charlotte , Gay Lauriane , Calas Bernard 
Published: June 2015

Le périmètre rizicole d’Olweny dans le nord de l’Ouganda est une opération d’aménagement du territoire destinée non à réduire l’insécurité alimentaire nationale mais à améliorer les revenus paysans pour réduire les disparités entre les agriculteurs et les urbains, les nordistes et les sudistes. Ce projet hérite de deux phases antérieures qui se sont soldées par des échecs à cause, d’une part, de l’insécurité civile qui a régné en Ouganda et plus particulièrement dans le Lango depuis 1980, et, d’autre part, des erreurs de conceptions initiales. En 2014, dans le cadre de la démocratie pluripartite, la réhabilitation du projet participe de la stratégie du pouvoir pour conquérir l’électorat du Nord, traditionnellement dans l’opposition. Localement, le projet pose trois enjeux. Le premier est l’émergence d’un consensus autour de la gestion du projet et d’une mise en cohérence des pratiques agraires, le second concerne l’obligation d’augmenter le capital travail. Enfin le troisième concerne la marchandisation de la terre qu’accélère le projet.
Accelerated Corneal Collagen Cross-linking for Postoperative LASIK Ectasia: Two-Year Outcomes
Authors: Marino Gustavo K. , Torricelli Andre A. M. , Giacomin Natalia , Santhiago Marcony R. , Espindola Rodrigo , Netto Marcelo V. 
Published: June 2015

PURPOSE:
To evaluate the effectiveness and safety of accelerated corneal collagen cross-linking for postoperative LASIK ectasia after 2 years.

METHODS:

A prospective, single-center case series was performed with patients treated for postoperative LASIK ectasia. All eyes underwent accelerated corneal collagen cross-linking (CCL-Vario Crosslinking; Peschke Meditrade GmbH, Zurich, Switzerland) at 9 mW/cm
2
for 10 minutes. The main outcome measures were changes in uncorrected distance visual acuity, corrected distance visual acuity, central corneal thickness, corneal topography, and endothelial cell density. These parameters were assessed at baseline and at the 6-month and 1- and 2-year follow-up visit.

RESULTS:

The study enrolled 40 eyes of 24 patients (15 male and 9 female) with a mean age of 33.8 ± 7.5 years (range: 24 to 52 years) that attained at least 2 years of follow-up. The surgical procedure was uneventful in all cases. All eyes stabilized after treatment without any further signs of progression and no statistically significant changes in the mean uncorrected distance visual acuity (
P
= .649), corrected distance visual acuity (
P
= .616), mean keratometry (
P
=.837), steep keratometry (
P
= .956), ultrasonic pachymetry (
P
= .135), slit-scanning pachymetry (
P
= .276), and endothelial cell density (
P
= .523). In addition, 72.5% of the patients presented stable or gains of Snellen lines over time.

CONCLUSIONS:
Accelerated corneal collagen cross-linking seems to be safe and effective in halting postoperative LASIK ectasia progression after 2 years of follow-up. However, a longer follow-up period with a larger cohort is needed to validate these findings.

[
J Refract Surg.
2015;31(6):380–384.]
Intrastromal Corneal Ring Segment Explantation in Patients With Keratoconus: Causes, Technique, and Outcomes
Authors: Chhadva Priyanka , Yesilirmak Nilufer , Cabot Florence , Yoo Sonia H. 
Published: June 2015

PURPOSE:
To assess the causes for intrastromal corneal ring segment (Intacs; Addition Technology Inc., Lombard, IL) explantation in patients with keratoconus, and technique for explantation, long-term outcomes, and secondary procedures to correct visual acuity.

METHODS:
Ten eyes of 8 patients with a history of Intacs explantation between 2004 and 2012 were included in a retrospective study performed at the Bascom Palmer Eye Institute, Miami, Florida. Causes of Intacs removal, surgical technique, preoperative and postoperative corneal examination, and uncorrected and corrected distance visual acuity were documented. Additionally, corneal topography (Tomey, Nagoya, Japan) parameters such as average keratometry and corneal cylinder were assessed.

RESULTS:

Although the segments were well positioned, the most common cause of Intacs removal was worsening visual acuity (80%). There was no statistically significant difference between pre-Intacs placement, post-Intacs placement, and post-Intacs removal in uncorrected and corrected distance visual acuity, average keratometry, or corneal cylinder, except between 1-year post-Intacs placement corrected distance visual acuity (0.57 logMAR [20/75 Snellen]) and 1-month post-Intacs removal corrected distance visual acuity (0.25 logMAR [20/36 Snellen],
P
=.03). Four patients underwent penetrating keratoplasty after Intacs removal with good visual outcomes.

CONCLUSION:
This study demonstrates the visual and structural outcomes that returned to near baseline after Intacs explantation in keratoconic eyes.

[
J Refract Surg.
2015;31(6):392–397.]
Estimativas de parâmetros genéticos e a variabilidade em procedências e progênies de Handroanthus vellosoi
Authors: Batista Camila Moreira , Freitas Miguel Luiz Menezes , Moraes Marcela Aparecida de , Zanatto Antonio Carlos Scatena , Santos Pedro César dos , Zanata Marcelo , Moraes Mario Luiz Teixeira de , Sebbenn Alexandre Magno 
Published: June 2015

O presente estudo objetivou estimar parâmetros genéticos e a variabilidade entre e dentro de procedências, para caracteres silviculturais em um teste de procedências e progênies de Handroanthus vellosoi (Toledo) Mattos estabelecido na Estação Experimental de Luiz Antônio (SP). O teste foi instalado no delineamento experimental de blocos de famílias compactas, com duas procedências (alocadas nas parcelas), seis repetições e sub-parcelas lineares de cinco plantas, totalizando 35 progênies. O ensaio foi mensurado para os caracteres diâmetro à altura do peito, altura de planta, volume cilíndrico, forma do fuste e sobrevivência aos 24 anos de idade. Diferenças significativas entre procedências foram observadas apenas para o caráter forma do fuste. Pela análise de deviance foram detectadas diferenças significativas para o efeito de progênies para análise conjunta das procedências (população experimental), sugerindo que esta tem alta variação genética e indicando possibilidade de melhoramento genético pela seleção das melhores progênies. A estimativa de herdabilidade em nível individual e média de progênies variaram de baixa a moderada (26% para DAP, 32% para altura, 24% para volume cilíndrico, 27% para forma do fuste e 46% para sobrevivência), confirmando a possibilidade de melhoramento genético por seleção e que as procedências tem potencial para responder à pressão de seleção natural.
Análisis del uso e integración de redes sociales colaborativas en comunidades de aprendizaje de la Universidad de Granada (España) y John Moores de Liverpool (Reino Unido)
Authors: Trujillo Torres Juan Manuel , Aznar Díaz Inmaculada , Cáceres Reche María Pilar 
Published: June 2015

En la actualidad, la integración de redes sociales educativas está adquiriendo un papel fundamental, para la mejora de los procesos de Enseñanza-Aprendizaje (E-A) en el ámbito universitario. El presente artículo sintetiza las aportaciones más relevantes recogidas a través de un estudio descriptivo, centrado en las percepciones del alumnado mediante la administración de un cuestionario y grupos de discusión, inmersos en la red educativa UGR-John Moores, como experiencia de investigación innovadora en la enseñanza universitaria. El principal objetivo del estudio es conocer el uso e integración de esta red social y educativa, y valorar las posibilidades del aprendizaje colaborativo, para indagar y mejorar las acciones innovadoras desarrolladas en las nuevas metodologías docentes, abordadas en estas dos instituciones universitarias. Entre las contribuciones más destacables encontramos que la utilización de la red UGR/John Moores ha tenido una gran aceptación entre el alumnado, considerando la plataforma como un elemento facilitador para su aprendizaje, capaz de motivarle, generar y compartir sistemáticamente información, aumentando con todo ello, su entorno personal de aprendizaje (PLE). Sin embargo, es preciso incorporar una serie de mejoras respecto a la planificación docente con la integración de las TIC (acción tutorial), así como actualizar la formación del alumnado en uso de herramientas tecnológicas.
CHEMICAL DEGRADATION OF POLYURETHANE FOAM WASTE AND ITS CROSSLINKED NATURAL RUBBER
Authors: Riyajan Sa-Ad , Keawmwnee Nattanan , Tangboriboonrat Pramuan 
Published: June 2015

ABSTRACT
The objective of this work was to use by-products from the chemical degradation of polyurethane foam waste (w-PUfd) and blend them with an epoxidized natural rubber (ENR), named PENR, for improving the mechanical properties of a Standard Thai rubber 5L (STR 5L). The swelling ratio in toluene of the PENR decreased as a function of w-PUfd content due to an increase in chemical interactions. After blending the PENR with the STR-5L and vulcanizing agents using two-roll mill and then compression molding at 150 °C, the effects of w-PUfd content in PENR on the properties of cured STR-5L were investigated. The cure rate index, tensile strength, and elongation at break of the cured STR-5L were improved after the addition of PENR. The swelling in toluene of cured STR-5L decreased as a function of w-PUfd in PENR. At 100 phr w-PUfd in PENR, the 20:80 PENR:STR-5L provided the highest tensile strength of ~23 MPa. These biodegradable, cured rubbers with w-PUfd content are environmental friendly and cost-effective products.
Efficacy of Adjunctive Mitomycin C in Transcanalicular Diode Laser Dacryocystorhinostomy in Different Age Groups
Authors: Kar Taner , Yildirim Yildiray , Topal Tuncay , Çolakoğlu Kadir , Ünal Melih Hamdi 
Published: June 2015

Purpose To evaluate the efficacy of adjunctive mitomycin C (MMC) in transcanalicular multidiode laser dacryocystorhinostomy (TCL-DCR) in different age groups. Methods Ninety-six eyes of 96 patients who underwent TCL-DCR for the treatment of nasolacrimal duct obstruction were included in this retrospective, comparative study. Patients were divided into 4 groups based on age and intraoperative use of MMC: group 1, TCL-DCR without MMC in the 20- to 44-year age group; group 2, TCL-DCR with MMC in the 20- to 44-year age group; group 3, TCL-DCR without MMC in the 45- to 76-year age group; group 4, TCL-DCR with MMC in the 45- to 76-year age group. The postoperative evaluation consisted of calculating and comparing the success rates between groups. Results Success rates at the final visit were 50% for group 1, 66.66% for group 2, 79.16% for group 3, and 84.61% for group 4. The differences between group 1 and group 4, and group 1 and group 3, were significant (p = 0.01 and p = 0.038, respectively). Logistic regression showed that age group had significant effect on success rate (p = 0.013). However, use of MMC had no significant effect on success rate (p = 0.23). Conclusions The success rates of the TCL-DCR with MMC application were found to be higher than those of TCL-DCR without MMC in different age groups. However, the differences did not reach statistical significance. In addition, our study demonstrated that age may be a significant factor influencing the surgical outcome of TCL-DCR.
Natremia, Tonicity, and Conductivity Measurements in Hemodialyzed Patients
Authors: Gaillard François , Laplanche Sophie , Petitclerc Thierry 
Published: June 2015

Purpose Natremia is usually considered to reflect tonicity in non-hemodialyzed patients. Some hemodialysis monitors provide an online value (NaCond) of natremia calculated from conductivity measurements. This study compared the relation between tonicity and natremia (NaLab) measured at laboratory with the relation between tonicity and NaCond in hemodialysis patients. Methods Fifty-five hemodialysis sessions performed with a Fresenius 5008 dialysis monitor (Fresenius Medical Care, Bad Homburg, Germany) providing a value of NaCond were analyzed. Tonicity (calculated as “osmolality –urea”), NaLab and NaCond were measured at the beginning and end of sessions. Results The r2 correlation-coefficient between tonicity and NaLab is 0.48 (n = 110). The correlation between tonicity and NaCond is stronger (r2 = 0.71). Conclusions Conductivity measurements provide a natremia value (NaCond) that is a better surrogate for tonicity than natremia measured at laboratory. Because NaCond is not obtained from sodium measurement, dialysis monitors should display a value for plasma conductivity (mS/cm) instead for natremia (mmol/l).
3D Finite Element Modeling of Epiretinal Stimulation: Impact of Prosthetic Electrode Size and Distance from the Retina
Authors: Sui Xiaohong , Huang Yu , Feng Fuchen , Huang Chenhui , Chan Leanne Lai Hang , Wang Guoxing 
Published: June 2015

Purpose A novel 3-dimensional (3D) finite element model was established to systematically investigate the impact of the diameter (Φ) of disc electrodes and the electrode-to-retina distance on the effectiveness of stimulation. Methods The 3D finite element model was established based on a disc platinum stimulating electrode and a 6-layered retinal structure. The ground electrode was placed in the extraocular space in direct attachment with sclera and treated as a distant return electrode. An established criterion of electric-field strength of 1000 Vm−1 was adopted as the activation threshold for RGCs. Results The threshold current (TC) increased linearly with increasing Φ and electrode-to-retina distance and remained almost unchanged with further increases in diameter. However, the threshold charge density (TCD) increased dramatically with decreasing electrode diameter. TCD exceeded the electrode safety limit for an electrode diameter of 50 μm at an electrode-to-retina distance of 50 to 200 μm. The electric field distributions illustrated that smaller electrode diameters and shorter electrode-to-retina distances were preferred due to more localized excitation of RGC area under stimulation of different threshold currents in terms of varied electrode size and electrode-to-retina distances. Under the condition of same-amplitude current stimulation, a large electrode exhibited an improved potential spatial selectivity at large electrode-to-retina distances. Conclusions Modeling results were consistent with those reported in animal electrophysiological experiments and clinical trials, validating the 3D finite element model of epiretinal stimulation. The computational model proved to be useful in optimizing the design of an epiretinal stimulating electrode for prosthesis.
Solubility and pH of Direct pulp Capping Materials: A Comparative Study
Authors: Poggio Claudio , Lombardini Marco , Colombo Marco , Beltrami Riccardo , Rindi Simonetta 
Published: June 2015

Background The objective of the present study was to compare solubility and pH of 6 direct pulp capping materials. Methods Specimens of each material – i.e., Dycal, Calcicur, Calcimol LC, TheraCal LC, MTA Angelus and ProRoot MTA – were prepared and immersed in water. Solubility was determined after 24 hours and 2 months and analyzed statistically using a 1-way ANOVA and post hoc Tukey test. pH values were measured 3 and 24 hours after manipulation. Results All direct pulp capping materials showed low solubility; the pH of tested materials ranged from 10 to 12 and showed a nonsignificant increase/reduction after 24 hours. Conclusions Within the limitations of this in vitro study, the direct pulp capping materials studied showed different solubility even if no changes were recorded over time. All of the materials showed a very alkaline pH.
Safety and efficacy of combined micropuncture and shallow angle femoral artery access for neurovascular angiography
Authors: Bourgeois Austin C. , Kolze Chris T. , Guimaraes Marcelo S. , Pasciak Alexander S. , Ferrell Andrew S. , Bradley Yong , Kvamme Peter 
Published: June 2015

Introduction The AXERA 2 low-angle vascular access device utilizes a dual arteriotomy mechanism in which the standard access tract is compressed by a vascular sheath inserted over the second, low-angle tract. It is unknown whether this device could be effectively used with 21-gauge micropuncture access, as the micropuncture introducer makes a larger arteriotomy than the 19-gauge needle provided with the AXERA 2 system. Materials and Methods A retrospective review was performed on 189 patients who underwent common femoral artery access for diagnostic cerebrovascular angiography using either combined micropuncture and AXERA 2 access or standard access with manual pressure hemostasis. Demographic and procedural data were reviewed along with complications related to vascular access and times to bed elevation, ambulation and discharge. Results Combined micropuncture and AXERA 2 access was performed on 110 patients and 79 patients had standard access. The AXERA device was successfully used in 91.8% of the cases. Demographic data, anticoagulant use and sheath sizes were similar between both subsets. Use of the AXERA 2 was associated with two bleeding complications (1.8%) compared with 10 (12.7%) with manual pressure hemostasis alone. Institution-specific protocol allowed shorter mean manual compression time, as well as shorter times to ambulation and discharge with the AXERA 2. Conclusions Use of the AXERA 2 device with micropuncture access did not infer increased bleeding risk than standard arterial access in this patient series. The considerable incidence of device use failures suggests a learning curve associated with its use.
Invasion and Anti-Invasion Research of Glioma Cells in An Improved Model of Organotypic Brain Slice Culture
Authors: Ren Bingcheng , Yu Shengping , Chen Cong , Wang Le , Liu Zhifeng , Wu Qiaoli , Wang Leilei , Zhao Kai , Yang Xuejun 
Published: June 2015

Aims and background Although glioblastomas infiltrate diffusely into adjacent brain, it is difficult to unequivocally identify the solitary invading glioma cell. It is necessary to develop coculture models to study the motility of glioma cells, and to monitor the cellular morphology, movement direction, migration area and invasion rate. Methods Cerebral slices were cultured on Millicell-CM membrane inserts in a petri dish. The neuronal viability and organizational structure of the brain sections were well maintained by experimental verification. C6 cell clones with persistent enhanced green fluorescent protein (EGFP) expression were established. EGFP-expressing glioma cells were cultured to form aggregates, which were implanted on the brain slices. The invasion area and migration rates of C6 cells on brain slices were measured. We evaluated the invasion area and depth after C6 cells were treated with the Rac1 inhibitor NSC23766. Results We successfully established the glioma cell-brain slice coculture model. In coculture, the average migration rate of C6 glioma cells within brain slices reached 11.36-15.27 urn/hour. The polarity of C6 glioma cells was parallel to the white matter tracts after 7 days. The invasive ability of C6 cells (depth: 105.3 ± 10.3 μm) treated with NSC23766 was weakened compared with the control group (depth: 198 ± 9.2 μm) within the white matter of brain slices (t = 16.26, p<0.05). Conclusions We developed the model to analyze the invasion features of glioma cells. The significant suppression of glioma cell invasion by NSC23766 in brain slices indicates that anti-Rac1 treatment may represent an important future therapeutic strategy for glioblastoma.