bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synchronized dynamics of bacterial niche‐specific functions during biofilm development in a cold seep brine pool
Authors: Zhang Weipeng , Wang Yong , Bougouffa Salim , Tian Renmao , Cao Huiluo , Li Yongxin , Cai Lin , Wong Yue Him , Zhang Gen , Zhou Guowei , Zhang Xixiang , Bajic Vladimir B. , Al‐Suwailem Abdulaziz , Qian Pei‐Yuan 
Published: July 2015

SummaryThe biology of biofilm in deep‐sea environments is barely being explored. Here, biofilms were developed at the brine pool (characterized by limited carbon sources) and the normal bottom water adjacent to Thuwal cold seeps. Comparative metagenomics based on 50 Gb datasets identified polysaccharide degradation, nitrate reduction and proteolysis as enriched functional categories for brine biofilms. The genomes of two dominant species: a novel Deltaproteobacterium and a novel Epsilonproteobacterium in the brine biofilms were reconstructed. Despite rather small genome sizes, the Deltaproteobacterium possessed enhanced polysaccharide fermentation pathways, whereas the Epsilonproteobacterium was a versatile nitrogen reactor possessing nar, nap and nif gene clusters. These metabolic functions, together with specific regulatory and hypersaline‐tolerant genes, made the two bacteria unique compared with their close relatives, including those from hydrothermal vents. Moreover, these functions were regulated by biofilm development, as both the abundance and the expression level of key functional genes were higher in later stage biofilms, and co‐occurrences between the two dominant bacteria were demonstrated. Collectively, unique mechanisms were revealed: (i) polysaccharides fermentation, proteolysis interacted with nitrogen cycling to form a complex chain for energy generation, and (ii) remarkably exploiting and organizing niche‐specific functions would be an important strategy for biofilm‐dependent adaptation to the extreme conditions.
Enhanced salinities, as a proxy of seawater desalination discharges, impact coastal microbial communities of the eastern Mediterranean Sea
Authors: Belkin Natalia , Rahav Eyal , Elifantz Hila , Kress Nurit , Berman‐Frank Ilana 
Published: July 2015

SummarySeawater desalination plants increase local coastal salinities by discharging concentrated brine back to the sea with ∼50% higher than ambient salinities. The impacts of high salinities on microbial coastal populations of the eastern Mediterranean Sea (EMS) were examined in two mesocosm experiments; first, during the mixed‐spring and second, during the stratified‐summer periods with average salinity of ∼39. Ambient salinities were increased by 5% and 15%. Higher salinity (15%) mesocosms induced rapid (within 2 h) declines in both primary productivity (PP) and algal biomass parallel to an increase in bacterial productivity. Subsequently, for the duration of the experiments (11–12 days), both Chlorophyll a and PP rates increased (2 to 5 and 1.5 to 2.5–fold, respectively) relative to unamended controls. The initial assemblages of the ambient microbial populations and intensity of salinity enrichments influenced the community responses. During the mixed‐spring experiment, the composition of prokaryotic and eukaryotic populations shifted only slightly, suggesting high functional plasticity of the initial populations. While during the stratified‐summer experiment, high salinity changed the composition and reduced the biodiversity of the microbial communities. In an ultra‐oligotrophic environment such as the EMS, salinity induced declines in microbial diversity may provide a tipping point destabilizing the local aquatic food web.
Microenvironment and phylogenetic diversity of Prochloron inhabiting the surface of crustose didemnid ascidians
Authors: Nielsen Daniel A. , Pernice Mathieu , Schliep Martin , Sablok Gaurav , Jeffries Thomas C. , Kühl Michael , Wangpraseurt Daniel , Ralph Peter J. , Larkum Anthony W. D. 
Published: July 2015

SummaryThe cyanobacterium Prochloron didemni is primarily found in symbiotic relationships with various marine hosts such as ascidians and sponges. Prochloron remains to be successfully cultivated outside of its host, which reflects a lack of knowledge of its unique ecophysiological requirements. We investigated the microenvironment and diversity of Prochloron inhabiting the upper, exposed surface of didemnid ascidians, providing the first insights into this microhabitat. The pH and O2 concentration in this Prochloron biofilm changes dynamically with irradiance, where photosynthetic activity measurements showed low light adaptation (Ek ∼80 ± 7 μmol photons m−2 s−1) but high light tolerance. Surface Prochloron cells exhibited a different fine structure to Prochloron cells from cloacal cavities in other ascidians, the principle difference being a central area of many vacuoles dissected by single thylakoids in the surface Prochloron. Cyanobacterial 16S rDNA pyro‐sequencing of the biofilm community on four ascidians resulted in 433 operational taxonomic units (OTUs) where on average −85% (65–99%) of all sequence reads, represented by 136 OTUs, were identified as Prochloron via blast search. All of the major Prochloron‐OTUs clustered into independent, highly supported phylotypes separate from sequences reported for internal Prochloron, suggesting a hitherto unexplored genetic variability among Prochloron colonizing the outer surface of didemnids.
Risk and self‐managing chronic joint pain: looking beyond individual lifestyles and behaviour
Authors: Morden Andrew , Jinks Clare , Ong Bie Nio 
Published: July 2015

AbstractSelf‐managing chronic musculoskeletal pain is predominantly framed within a discourse of modifying behaviour, or lifestyle risk factors such as diet, weight loss and exercise by policymakers, researcher and clinicians. Little research has been conducted which explores how ‘risk’ is understood or encountered by those with joint pain and how it may relate to self‐management. Drawing from serial interviews and a diary study with 22 participants, the findings demonstrate that people with chronic pain engage in a process of assessing and adapting to hazardous or pain conferring situations in relation to daily activities. ‘Risks’ are embedded within a dialectic between corporeal experience and the design features of everyday social environments. Self‐management, in this context, is not necessarily solely related to following clinical advice, rather it includes dealing with ‘risks’ of pain, hazards relating to bodily limitations and the environment, and ensuring the ability to continue with valued activities. Findings contribute to sociological understandings of self‐management and risk while demonstrating the limits of viewing self‐management as an individualised endeavour of changing behaviour.
‘Many Rivers to Cross’: Suburban Densification and the Social Status Quo in Greater Lyon
Authors: Rousseau Max 
Published: July 2015

AbstractUrban research often considers densification from the perspective of sustainable development and social mix. This essay focuses instead on the social and political stakes involved in densification through the example of a large French metropolitan area. It shows that the densification policies put in place in the Lyon agglomeration cannot be said to succeed in breaking down the historical segregation between its residential and affluent western suburbs (banlieues) and its industrial and working‐class eastern ones. The political manoeuvres executed by the institutions implementing densification, and the search for consensus characterizing France's intercommunalities, block any possibility of redistributing functions and social classes at the metropolitan scale, and hence of ending the social specialization of Lyon's suburbs. Moreover, municipalities subjected to pressure from suburban areas carefully assess the profile of residents selected to occupy new housing units—i.e. individuals already residing in the commune in the case of western suburbs, and middle‐class individuals hailing from the eastern part of the agglomeration in the case of eastern suburbs. Densification does not foster social mix at the metropolitan scale, neither does it improve the housing conditions of disadvantaged populations.
Santiago Calatrava and the ‘Power of Faith’: Global Imaginaries in Valencia
Authors: Tarazona Vento Amparo 
Published: July 2015

AbstractThe focus of this article is on the importance of collective imaginaries for urban policy mobility, and on the agents and modes of power through which imaginaries are translated, mobilized and become materialized in specific places. Through the case study of the monumental complex of the City of Arts and Sciences in Valencia, designed by Valencian global architect Santiago Calatrava, I discuss Calatrava's mobilization of ideas of modernity, tradition, democratization and self‐esteem already present in the collective imaginary of the people and the politicians of post‐Franco's Spain to promote his global architecture. In this process, I argue, global imaginaries were translated into local imaginaries and vice versa as they became embodied in persons and objects, represented in this case by Calatrava's figure as a local/global architect and by the architecture of the City of Arts and Sciences. Particularly, I analyse Calatrava's use of the power of seduction, persuasion and coercion to mobilize such imaginaries in order to build his ubiquitous signature architecture in Valencia.
Practising family history: ‘identity’ as a category of social practice
Authors: Bottero Wendy 
Published: July 2015

AbstractResearch on family history argues it performs the task of anchoring a sense of ‘self’ through tracing ancestral connection and cultural belonging, seeing it as a form of storied ‘identity‐work’. This paper draws on a small‐scale qualitative study to think further on the identity‐work of family history. Using practice theory, and a disaggregated notion of ‘identity’, it explores how the storying of family histories relates to genealogy as a leisure hobby, a form of historical research, and an information‐processing activity; and examines the social organization of that narrativity, where various practical engagements render certain kinds of genealogical information more, or less, ‘storyable’. Key features of ‘identity‐work’ in family history, such as the construction of genealogy as a personal journey of discovery and identification with particular ancestors, emerge as a consequence of the procedures of family history, organized as a set of practical tasks. The paper explores ‘identity‐work’ as a consequence of people's engagement in specific social practices which provide an internal logic to their actions, with various components of ‘identity’ emerging as categories of practice shaped within, and for, use. Focusing on ‘identity’ as something produced when we are engaged in doing other things, the paper examines how the practical organization of ‘doing other things’ helps produce ‘identity’ in particular ways.
Frequency, severity and risk factors for urinary and faecal incontinence at 4 years postpartum: a prospective cohort
Authors: Gartland D , MacArthur C , Woolhouse H , McDonald E , Brown SJ 
Published: July 2015

ObjectivesTo investigate frequency, severity and risk factors for urinary incontinence and faecal incontinence 4 years after a first birth.DesignProspective pregnancy cohort study.SettingMelbourne, Australia.SampleA total of 1011 nulliparous women recruited in early pregnancy.MethodsParticipants were followed up at 32 weeks of gestation; then at 3, 6, 9 and 12 months and 4 years postpartum.Main outcome measuresFrequency and severity of urinary and faecal incontinence.ResultsAt 4 years, 29.6% of women reported urinary incontinence and 7.1% reported faecal incontinence. Compared with women having only spontaneous vaginal births, women who delivered exclusively by caesarean section were less likely to have urinary incontinence at 4 years postpartum (adjusted odds ratio 0.4, 95% confidence interval 0.3–0.6). Women who reported urinary incontinence before or during the index pregnancy, and those experiencing symptoms in the first year postpartum had increased odds of incontinence at 4 years, with the highest odds (6–12 times higher) among women who had previously reported moderate or severe symptoms. The odds of reporting faecal incontinence at 4 years were two to six times higher for women experiencing symptoms in pregnancy, and around four to eight times higher for those with symptoms in the first year postpartum.ConclusionUrinary and faecal incontinence are prevalent conditions 4 years after a first birth. Women reporting urinary or faecal incontinence during pregnancy had markedly higher odds of reporting symptoms at 4 years postpartum, suggesting a need for further investigation and elucidation of aetiological pathways involving nonbirth‐related risk factors.Tweetable abstractModerate/severe incontinence prevalent 4 years after first birth in population cohort. Prior symptoms are biggest predictor.
Generosity in intergovernmental negotiations: The impact of state power, pooling and socialisation in the Council of the European Union
Authors: Naurin Daniel 
Published: July 2015

AbstractScholars have long emphasised the consensual nature of the intergovernmental negotiations in the Council of the European Union. Unlike other international organisations, where surface consensus has been found to be merely a cover for the dominance of powerful states, the EU literature describes a norm of generosity that works as a real constraining factor. In contrast, this article warns against descriptions of the EU as different in kind. Based on interviews with 231 EU Member State representatives involved in day‐to‐day negotiations in the Council, it finds a strong bias in generosity on behalf of the three dominant powers: France, Germany and the United Kingdom. The ‘Big 3’ are strikingly unwilling to make generous concessions, compared to other states. Furthermore, from a rational perspective, there are good reasons for expecting this pattern. The study also shows that extensive pooling of power in the form of qualified majority rule and hard law commitment is associated with less generosity, while there seems to be no socialising effect towards generosity from exposure to the ‘Brussels community’. These findings cast a new light on the common narrative of the EU as a ‘soft‐bargaining’ anomaly among international organisations, where national interests are upgraded into common interests by a process of norm socialisation. Instead, it seems that the purported ‘consensus norm’ has been far from successful in transcending fundamental power asymmetries between the EU Member States.
Chromatographic Methods for the Determination of Carbohydrates and Organic Acids in Foods of Animal Origin
Authors: Pereira da Costa Marion , Conte‐Junior Carlos Adam 
Published: July 2015

AbstractCarbohydrates are ubiquitous and range from simple monosaccharides to large complex polysaccharides. Organic acids are compounds with acidic properties. Both occur naturally in many foods and in fermented products. Organic acids are usually derived from the hydrolysis of carbohydrates by microorganisms such as lactic acid bacteria. These bacteria convert carbohydrates into energy required for growth, since they are not equipped with the enzymes necessary for respiration and are unable to perform oxidative phosphorylation. Determination of carbohydrates and organic acids in foods of animal origin is important, since they contribute to flavor and texture. Their presence and proportions can affect the chemical and sensory characteristics of a food matrix and they can provide information on nutritional properties of food and the means to optimize selected technological processes. Furthermore, the levels of carbohydrate and organic acid are important to monitor bacterial growth and activity. Actually, these compounds can be quantified by several methods including high‐performance liquid chromatography (HPLC) and gas chromatography (GC). High‐performance liquid chromatography has been widely used to analyze carbohydrates and nonvolatile organic acids, while gas chromatography has been used to determine the volatile organic acids in complex matrices. This contribution provides an overview of chromatographic methods (HPLC and GC) used to analyze carbohydrates and organic acids in foods of animal origin.
Forensic Evidence in Homicide Investigations and Prosecutions
Authors: McEwen Tom , Regoeczi Wendy 
Published: July 2015

AbstractEven though forensic evidence is collected at virtually every homicide scene, only a few studies have examined its role in investigation and prosecution. This article adds to the literature by providing the results of a study of 294 homicide cases (315 victims) occurring in Cleveland, Ohio, between 2008 and 2011. Through a logistic regression on open versus closed cases, the collection of knives, administration of gunshot residue (GSR) kits, and clothing at the scene were positively and significantly related to case closures, while collection of ballistics evidence andDNAevidence were statistically significant in the opposite direction. With regard to analysis, the clearance rate for cases with probative results (i.e., matches or exclusions) was 63.1% compared to a closure rate of 56.3% for cases without probative results. However, only 23 cases had probative results prior to arrest compared to 128 cases with probative results after arrest.
Quantitation of Synthetic Cannabinoids in Plant Materials Using High Performance Liquid Chromatography with UV Detection (Validated Method)
Authors: Ciolino Laura A. 
Published: July 2015

AbstractPlant based products laced with synthetic cannabinoids have become popular substances of abuse over the last decade. Quantitative analysis for synthetic cannabinoid content in the laced materials is necessary for health hazard assessments addressing overall exposure and toxicity when the products are smoked. A validated, broadly applicable HPLC‐UV method for the determination of synthetic cannabinoids in plant materials is presented, using acetonitrile extraction and separation on a commercial phenylhexyl stationary phase. UV detection provides excellent sensitivity with limits of quantitation (LOQs) less than 10 μg/g for many cannabinoids. The method was validated for several structural classes (dibenzopyrans, cyclohexylphenols, naphthoylindoles, benzoylindoles, phenylacetylindoles, tetramethylcyclopropylindoles) based on spike recovery experiments in multiple plant materials over a wide cannabinoid contents range (0.1–81 mg/g). Average recovery across 32 cannabinoids was 94% for marshmallow leaf, 95% for damiana leaf, and 92% for mullein leaf. The method was applied to a series of case‐related products with determined amounts ranging from 0.2 to >100 mg/g.
Taxonomy of Challenges for Digital Forensics
Authors: Karie Nickson M. , Venter Hein S. 
Published: July 2015

AbstractSince its inception, over a decade ago, the field of digital forensics has faced numerous challenges. Despite different researchers and digital forensic practitioners having studied and analysed various known digital forensic challenges, as of 2013, there still exists a need for a formal classification of these challenges. This article therefore reviews existing research literature and highlights the various challenges that digital forensics has faced for the last 10 years. In conducting this research study, however, it was difficult for the authors to review all the existing research literature in the digital forensic domain; hence, sampling and randomization techniques were employed to facilitate the review of the gathered literature. Taxonomy of the various challenges is subsequently proposed in this paper based on our review of the literature. The taxonomy classifies the large number of digital forensic challenges into four well‐defined and easily understood categories. The proposed taxonomy can be useful, for example, in future developments of automated digital forensic tools by explicitly describing processes and procedures that focus on addressing specific challenges identified in this paper. However, it should also be noted that the purpose of this paper was not to propose any solutions to the individual challenges that digital forensics face, but to serve as a survey of the state of the art of the research area.
Sources and Transport of Phosphorus to Rivers in California and Adjacent States, U.S., as Determined by SPARROW Modeling
Authors: Domagalski Joseph , Saleh Dina 
Published: July 2015

AbstractThe SPARROW (SPAtially Referenced Regression on Watershed attributes) model was used to simulate annual phosphorus loads and concentrations in unmonitored stream reaches in California, U.S., and portions of Nevada and Oregon. The model was calibrated using de‐trended streamflow and phosphorus concentration data at 80 locations. The model explained 91% of the variability in loads and 51% of the variability in yields for a base year of 2002. Point sources, geological background, and cultivated land were significant sources. Variables used to explain delivery of phosphorus from land to water were precipitation and soil clay content. Aquatic loss of phosphorus was significant in streams of all sizes, with the greatest decay predicted in small‐ and intermediate‐sized streams. Geological sources, including volcanic rocks and shales, were the principal control on concentrations and loads in many regions. Some localized formations such as the Monterey shale of southern California are important sources of phosphorus and may contribute to elevated stream concentrations. Many of the larger point source facilities were located in downstream areas, near the ocean, and do not affect inland streams except for a few locations. Large areas of cultivated land result in phosphorus load increases, but do not necessarily increase the loads above those of geological background in some cases because of local hydrology, which limits the potential of phosphorus transport from land to streams.
Quantifying Tradeoffs Associated with Hydrologic Environmental Flow Methods
Authors: McKay S. Kyle 
Published: July 2015

AbstractFreshwater management requires balancing and tradingoff multiple objectives, many of which may be competing. Ecological needs for freshwater are often described in terms of environmental flow recommendations (e.g., minimum flows), and there are many techniques for developing these recommendations, which range from hydrologic rules to multidisciplinary analyses supported by large teams of subject matter experts. Although hydrologic rules are well acknowledged as overly simplified, these techniques remain the state‐of‐the‐practice in many locations. This article seeks to add complexity to the application of these techniques by studying the emergent properties of hydrologic environmental flow methodologies. Two hydrologic rules are applied: minimum flow criteria and sustainability boundaries. Objectives and metrics associated with withdrawal rate and similarity to natural flow regimes are used to tradeoff economic and environmental needs, respectively, over a range of flow thresholds and value judgments. A case study of hypothetical water withdrawals on the Middle Oconee River near Athens, Georgia is applied to demonstrate these techniques. For this case study, sustainability boundaries emerge as preferable relative to both environmental and economic outcomes. Methods applied here provide a mechanism for examining the role of stakeholder values and tradeoffs in application of hydrologic rules for environmental flows.