bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of low‐dose photodynamic therapy using the photosensitizer chloroaluminum phthalocyanine on a scratch wound model in skin fibroblasts
Authors: Giannakopoulos Efstathios , Katopodi Annita , Rallis Michail , Politopoulos Konstantinos , Alexandratou Eleni 
Published: July 2024

AbstractDifferent approaches on wound healing have been developed over the years but they suffer from high costs and adverse effects for the patients. The current paper was designed to study low dose PDT, a novel healing approach, in an in vitro fibroblasts wound healing model. Chloroaluminum phthalocyanine (AlClPc) was used as photosensitizer and was activated by a red diode laser at 661 nm. After PDT optimization, wound closure rate and reactive oxygen species were quantified by image processing and analysis. Our results revealed that wound healing rates were significantly higher in PDT treated groups than in the control. Additionally, the study revealed that a prolonged ROS increase did not promote wound closure, while a small increase acted as a trigger, resulting in faster wound closure. Concluding, low dose PDT using AlClPc enhances wound healing in vitro in a ROS dependent manner, allowing the assumption of similar positive effects in vivo.
Dulaglutide reduces oxidative DNA damage and hypermethylation in the somatic cells of mice fed a high‐energy diet by restoring redox balance, inflammatory responses, and DNA repair gene expressions
Authors: Attia Sabry M. , Alshamrani Ali A. , Ahmad Sheikh F. , Albekairi Norah A. , Nadeem Ahmed , Attia Mohamed S. M. , Ansari Mushtaq A. , Almutairi Faris , Bakheet Saleh A. 
Published: July 2024

AbstractObesity is an established risk factor for numerous malignancies, although it remains uncertain whether the disease itself or weight‐loss drugs are responsible for a greater predisposition to cancer. The objective of the current study was to determine the impact of dulaglutide on genetic and epigenetic DNA damage caused by obesity, which is a crucial factor in the development of cancer. Mice were administered a low‐fat or high‐fat diet for 12 weeks, followed by a 5‐week treatment with dulaglutide. Following that, modifications of the DNA bases were examined using the comet assay. To clarify the underlying molecular mechanisms, oxidized and methylated DNA bases, changes in the redox status, levels of inflammatory cytokines, and the expression levels of some DNA repair genes were evaluated. Animals fed a high‐fat diet exhibited increased body weights, elevated DNA damage, oxidation of DNA bases, and DNA hypermethylation. In addition, obese mice showed altered inflammatory responses, redox imbalances, and repair gene expressions. The findings demonstrated that dulaglutide does not exhibit genotoxicity in the investigated conditions. Following dulaglutide administration, animals fed a high‐fat diet demonstrated low DNA damage, less oxidation and methylation of DNA bases, restored redox balance, and improved inflammatory responses. In addition, dulaglutide treatment restored the upregulated DNMT1, Ogg1, and p53 gene expression. Overall, dulaglutide effectively maintains DNA integrity in obese animals. It reduces oxidative DNA damage and hypermethylation by restoring redox balance, modulating inflammatory responses, and recovering altered gene expressions. These findings demonstrate dulaglutide's expediency in treating obesity and its associated complications.
Simple and low‐cost microscopy setup for 3D particle field measurement using incoherent illumination and open‐source hardware
Authors: Li Shengfu , Zhao Yu , Wen Weifeng , Xiong Chuanzhong , Meng Jianhua , Chen Guanghua , Zhou Pingwei , Zhu Yu , Gao Peng , Ye Yan 
Published: July 2024

AbstractThe quantification of 3D particle field is of interest for a vast range of fields. While in‐line particle holography (PH) can provide high‐resolution measurements of particles, it suffers from speckle noise. Plenoptic imaging (PI) is less susceptible to speckle noises, but it involves a trade‐off between spatial and angular resolution, rendering images with low resolution. Here, we report a simple microscopy setup with the goals of getting the strengths of both techniques. It is built with off‐the‐shelf and cost‐effective components including a photographic lens, a diaphragm, and a CCD camera. The cost of the microscopy setup is affordable to small labs and individual researchers. The pupil plane of the proposed setup can be mechanically accessible, allowing us to implement pupil plane modulation and increase the depth of field (DOF) without requiring any additional relay lenses. It also allows us to understand the working principle of pupil plane modulation clearly, benefiting microscopy education. It illuminates the sample (particles) using diffuse white light, and thus avoids the problem of speckle noise. It captures multiple perspective images via pupil plane modulation, without requiring trading off angular and spatial resolution. We validate the setup with 2D and 3D particle samples.Research HighlightsWe report a simple and cost‐effective microscopy setup with the goals of getting the strengths of plenoptic imaging and in‐line particle holography. It is built with off‐the‐shelf and cost‐effective components. The cost of the microscopy setup is affordable to small labs and individual researchers. The pupil plane of the proposed setup can be mechanically accessible, allowing us to implement pupil plane modulation and increase the DOF without requiring any additional relay lenses. It also allows us to understand the working principle of pupil plane modulation clearly, benefiting microscopy education. It illuminates the sample (particles) using diffuse white light, and thus avoids the problem of speckle noise. It captures multiple perspective images via pupil plane modulation, without requiring trading off angular and spatial resolution. We validate the setup with 2D and 3D particle samples.
A multi‐analytical investigation of Imperial (I‐II century AD) Roman cooking ware from ‘Villa della Piscina’ and the so called ‘Minerva Medica Temple’ (Rome, Italy)
Authors: Privitera Antonella , Bernardini Simone , Ventura Giancarlo Della , Ballirano Paolo , Arbolino Elena , Coletti Caterina , Conte Lorenzo , Pacetti Francesco , Morretta Simona , Sodo Armida 
Published: July 2024

AbstractA multi‐analytical approach based on colourimetry, micro‐Raman spectroscopy, scanning electron microscopy, optical microscopy and powder X‐ray diffraction, has been applied to investigate Roman cooking ware samples dating back to imperial age (I‐II century AD). In particular, the highly distinctive production of pots coming from two different archaeological sites, ‘Villa della Piscina’ at Centocelle district and the so called ‘Minerva Medica Temple’ at Esquilino district (Rome, Italy), was studied characterising the colour, the chemical, mineralogical and petrographic distinctive properties of the investigated samples, in order to compare technological and provenance aspects. Classification of ceramic fragment by colorimetry, integrated by compositional studies with a main contribution of micro‐Raman spectroscopy, allows to discriminate between superior and inferior quality cooking ware and evaluate the compatibility of the investigated samples with some pottery realised in known manufacturing sites in Lazio. Compositional and petrographic features allow assignment of samples from ‘Villa della Piscina’ testifying productions in the surroundings of Rome, while an importation from outsider workshops is hypothesised for the ceramic fragments from the so called ‘Minerva Medica Temple’. For these, an importation from outsider workshop is hypothesised.
An underutilized bean: hyacinth bean [Lablab purpureus (L.) sweet]: bioactive compounds, functional activity, and future food prospect and applications
Authors: Zhou Jiankang , Wang Wenting , Zhang Zhuo , Zhu Gege , Qiao Jiawei , Guo Shengyuan , Bai Yu , Zhao Chaofan , Teng Cong , Qin Peiyou , Zhang Lizhen , Ren Guixing 
Published: July 2024

AbstractHyacinth bean [Lablab purpureus (L.) Sweet], a plant belonging to the leguminous family and traditionally used for medicinal purposes in China, is a valuable resource with a wide range of health benefits. This review examines the bioactive compounds, health‐promoting properties and functional food potential of hyacinth bean, highlighting its role in protecting against metabolic diseases and the underlying molecular mechanisms. According to existing research, hyacinth bean contains a diverse array of bioactive compounds, Consumption of hyacinth beans and hyacinth bean‐related processed food products, as well as their use in medicines, is associated with a variety of health benefits that are increasingly favoured by the scientific community. In light of these findings, we posit that hyacinth bean holds great promise for further research and food application. © 2024 Society of Chemical Industry.
Platelet‐rich plasma injections for the management of knee osteoarthritis: The ESSKA‐ICRS consensus. Recommendations using the RAND/UCLA appropriateness method for different clinical scenarios
Authors: Kon Elizaveta , de Girolamo Laura , Laver Lior , Andriolo Luca , Andia Isabel , Bastos Ricardo , Beaufils Philippe , Biant Leela , Bøe Berte , Boffa Angelo , Cugat Ramon , Di Martino Alessandro , Erggelet Christoph , Iosifidis Michael , Kocaoglu Baris , Magalon Jérémy , Marinescu Rodica , Nehrer Stefan , Niemeyer Philipp , Ostojić Marko , Piontek Tomasz , Sánchez Mikel , Sas Kristof , Skarpas Georges , Tischer Thomas , Vonk Lucienne , Filardo Giuseppe 
Published: July 2024

AbstractPurposeThe aim of this consensus was to develop evidence‐ and expert‐based patient‐focused recommendations on the appropriateness of intra‐articular platelet‐rich plasma (PRP) injections in different clinical scenarios of patients with knee osteoarthritis (OA).MethodsThe RAND/UCLA Appropriateness Method was used by the European Society of Sports Traumatology, Knee Surgery, and Arthroscopy (ESSKA), as well as the International Cartilage Regeneration and Joint Preservation Society (ICRS) to reach a consensus and produce recommendations for specific patient categories combining best available scientific evidence with the collective judgement of a panel of experts.ResultsScenarios were defined based on first treatment vs first injective treatment vs second injective treatment, age (<50/50–65/66–80/>80), tibiofemoral vs patellofemoral involvement, OA level (Kellgren–Lawrence/KL 0–I/II–III/IV), and joint effusion (dry knee, minor‐mild or major effusion). Out of 216 scenarios, in 84 (38.9%) the indication was considered appropriate, in 9 (4.2%) inappropriate and in 123 (56.9%) uncertain. The parameters associated with the highest consensus were PRP use after failed injective treatments (62.5%), followed by PRP after failed conservative treatments and KL 0–III scenarios (58.3%), while the highest uncertainty was found for PRP use as first treatment and KL IV OA (91.7% and 87.5% of uncertain scenarios, respectively).ConclusionThis ESSKA‐ICRS consensus established recommendations on the appropriateness or inappropriateness of PRP injections for the treatment of knee OA, providing a useful reference for clinical practice. PRP injections are considered appropriate in patients aged ≤80 years with knee KL 0–III OA grade after failed conservative non‐injective or injective treatments, while they are not considered appropriate as first treatment nor in KL IV OA grade.Level of EvidenceLevel I.
The Onset of Lasing in Semiconductor Nanolasers
Authors: Saldutti Marco , Yu Yi , Mørk Jesper 
Published: July 2024

AbstractNanolasers based on emerging dielectric cavities with deep sub‐wavelength confinement of light offer a large light‐matter coupling rate and near‐unity spontaneous emission factor, . These features call for reconsidering the standard approach to identifying the lasing threshold. Here, a new threshold definition is suggested, taking into account the recycling process of photons when is large. This threshold reduces to the classical balance between gain and loss in the limit of macroscopic lasers, but qualitative as well as quantitative differences emerge for nanolasers. In particular, this new threshold identifies the onset of a transition regime, where the quantum statistics of the emitted light evolve into the Poissonian statistics of a coherent state. It is shown that the threshold with photon recycling consistently marks the onset of the change in the second‐order intensity correlation, , toward coherent laser light, irrespective of the laser size and down to the case of a single emitter. In contrast, other threshold definitions may well predict lasing in light‐emitting diodes. An overview of different threshold definitions proposed in the literature is provided and their predictions are compared when going from macroscopic to microscopic lasers.
2023 Inaugural Healthcare Delivery Science: Innovation and Partnerships for Health Equity Research (DESCIPHER) Symposium
Authors: Orechwa Allison Z. , Abhat Anshu , Amezcua Lilyana , Boden‐Albala Bernadette , Buchanan Thomas A. , Chen Steve , Daskivich Lauren P. , Feldman Brett , Gould Michael K. , Lee Wei‐an , Lynch Christopher , Meltzer Carolyn C. , Mittman Brian S. , Pereyda Margarita , Raff Evan , Robinson Jehni , Saluja Sonali , Turner Barbara J. , Taira Breena R. , Trotzky‐Sirr Rebecca , Williams Linda , Wu Shinyi , Yee Hal , Towfighi Amytis 
Published: July 2024

AbstractIntroductionThis article provides an overview of presentations and discussions from the inaugural Healthcare Delivery Science: Innovation and Partnerships for Health Equity Research (DESCIPHER) Symposium.MethodsThe symposium brought together esteemed experts from various disciplines to explore models for translating evidence‐based interventions into practice.ResultsThe symposium highlighted the importance of disruptive innovation in healthcare, the need for multi‐stakeholder engagement, and the significance of family and community involvement in healthcare interventions.ConclusionsThe article concluded with a call to action for advancing healthcare delivery science to achieve health equity.
A nomogram for predicting neurogenic lower urinary tract dysfunction in patients with acute ischemic stroke: A retrospective study
Authors: Hu Yingjie , Hao Fengming , Yu Lanlan , Chen Ling , Liang Surui , Wang Ying , Cai Wenzhi 
Published: July 2024

AbstractAimsTo investigate the risk factors for neurogenic lower urinary tract dysfunction (NLUTD) in patients with acute ischemic stroke (AIS), and develop an internally validated predictive nomogram. The study aims to offer insights for preventing AIS‐NLUTD.MethodsWe conducted a retrospective study on AIS patients in a Shenzhen Hospital from June 2021 to February 2023, categorizing them into non‐NLUTD and NLUTD groups. The bivariate analysis identified factors for AIS‐NLUTD (p < 0.05), integrated into a least absolute shrinkage and selection operator (LASSO) regression model. Significant variables from LASSO were used in a multivariate logistic regression for the predictive model, resulting in a nomogram. Nomogram performance and clinical utility were evaluated through receiver operating characteristic curves, calibration curves, decision curve analysis (DCA), and clinical impact curve (CIC). Internal validation used 1000 bootstrap resamplings.ResultsA total of 373 participants were included in this study, with an NLUTD incidence rate of 17.7% (66/373). NIHSS score (OR = 1.254), pneumonia (OR = 6.631), GLU (OR = 1.240), HGB (OR = 0.970), and hCRP (OR = 1.021) were used to construct a predictive model for NLUTD in AIS patients. The model exhibited good performance (AUC = 0.899, calibration curve p = 0.953). Internal validation of the model demonstrated strong discrimination and calibration abilities (AUC = 0.898). Results from DCA and CIC curves indicated that the prediction model had high clinical utility.ConclusionsWe developed a predictive model for AIS‐NLUTD and created a nomogram with strong predictive capabilities, assisting healthcare professionals in evaluating NLUTD risk among AIS patients and facilitating early intervention.
Successful utilization of clozapine for a patient with treatment‐resistant schizophrenia after recurrent violent behavior
Authors: Shinohara Rikuto Christopher , Oshima Tomomi , Otsubo Takafumi , Ariga Keita , Ono Tesshu , Muneoka Koya , Umezu Hiroki , Mikami Nobuhiro 
Published: July 2024

AbstractBackgroundIn patients with schizophrenia, violent behavior is a clinically important factor that prevents their discharge. Clozapine is an effective antipsychotic medication for treatment‐resistant schizophrenia, and its usefulness for aggressive behavior has also been suggested.Case PresentationWe present the case of a 38‐year‐old male patient diagnosed with schizophrenia who was successfully treated with clozapine after recurrent violent behavior. He was diagnosed with schizophrenia during his adolescence. He was hospitalized for treatment in his teens, but his hallucinations and delusions persisted even after discharge. In his 30s, he became noticeably emotionally unstable, and despite being treated for an adequate period with sufficient doses of several antipsychotics, his symptoms did not improve. This led to repeated hospitalizations triggered by violent behavior toward his parents and siblings within the home. During his fourth hospitalization, clozapine was initiated due to multiple incidents of violence toward nursing staff secondary to hallucinations and delusions. As the dose of clozapine was gradually increased with therapeutic drug monitoring, the patient's hostility, uncooperativeness, and suspiciousness markedly improved, and his aggressive behavior disappeared. He was discharged to a facility on day 194 after starting clozapine and has continued outpatient visits.ConclusionClozapine was suggested to be effective for aggressive behavior in patients with treatment‐resistant schizophrenia and should be actively considered. In such cases, regular measurement of blood concentration is useful for adjusting the dosage of clozapine.
Molecular Mechanisms of Buzhong Yiqi Decoction in the Treatment of Gastric Cancer: A Network Pharmacology, Molecular Docking, and In Vitro Experimental Analysis
Authors: Zeng Panke , Wu Xinyu , Chen Chen , Zhang Jianing , Rashid Haroon ur , Zhang Pengfei 
Published: July 2024

ABSTRACTGastric cancer (GC) is the most common type of cancer of the digestive system with high morbidity and mortality. Chemotherapy and targeted therapy are used to treat patients with advanced GC. However, side effects and drug resistance to the two modalities remain the main challenges. The Buzhong Yiqi decoction (BZYQD), a classical traditional Chinese medicine formula, has been reported for the treatment of various types of cancers. However, the underlying pharmacological mechanism has not been fully elucidated. Therefore, this study integrated network pharmacology, molecular docking, cancer public databases, and cell experiments to explore the potential bioactive compounds and BZYQD's mechanism of action against GC. A total of 245 targets of BZYQD, 5291 GC‐related targets, and 186 were identified as their common targets through the database. Network analysis confirmed AKT1, TP53, TNF, and EGFR to be the core targets, while the main compounds observed were quercetin, kaempferol, and β‐Sitosterol. The core signaling pathways included PI3K‐AKT, MAPK, TNF, and IL‐17. Molecular docking revealed good binding activity for the main compounds and core targets. Based on the database's validation of core targets, a large number of core genes were verified to be consistent with this study. Quercetin, kaempferol, and β‐Sitosterol were found to significantly reduce the growth of GC cells in the MTT experiment. The current study revealed that BZYQD may inhibit GC progression by interfering with core targets such as AKT1, TP53, TNF, EGFR, and MAPK3, and by regulating the activity of PI3K‐AKT, MAPK, TNF, and IL‐17 signaling pathways.
Comprehensive investigation on the synthesis of polyamide 6 and polyurethane elastomer blends via in situ polymerization
Authors: Hou Lianlong , Feng Bingtao , Wang Xiaoke , Zhang Meng , Yin Maofeng , Zhang Xin , Sun Guohua , Li Pengpeng , Ma Jinsong 
Published: July 2024

AbstractIn this work, blends of monomer‐cast polyamide 6 (MCPA6) and polyurethane (TPU) were prepared by in situ anionic ring‐opening polymerization using caprolactam as the monomer and sodium hydroxide (NaOH) as the initiator. TPU degraded under weakly alkaline conditions to form an isocyanate group, which acted as a macromolecule activator to initiate the growth of the MCPA6 chain, and the formation of TPU‐co‐MCPA6 copolymer in this system, which improves the compatibility between MCPA6 and TPU. The MCPA6/TPU blends were tested for conversion, viscosity, and mechanical properties, and their structures and thermal properties were analyzed and evaluated by characterization techniques such as Fourier transform infrared spectroscopy, differential scanning calorimetry, and scanning electron microscopy.
Temporal trends in medication and service use patterns for mental health issues among men with prostate cancer
Authors: Tiruye Tenaw , Hiwase Mrunal , Charlick Megan , O’Callaghan Michael , Khalid Ashna , Li Ming , FitzGerald Liesel M. , Caughey Gillian E. , Ettridge Kerry , Roder David , Beckmann Kerri 
Published: July 2024

AbstractObjectiveProstate cancer can significantly impact mental wellbeing, creating uncertainty and morbidity. This study described patterns of psychotropic medication and mental health service use, as a proxy measure for mental health problems, 5 years before and 5 years after prostate cancer diagnosis.MethodsPopulation‐based registry data were linked with Pharmaceutical Benefits Scheme and Medicare Benefits Schedule data for all prostate cancer patients diagnosed in South Australia between 2012 and 2020 (n = 13,693). We estimated the proportion and rates of psychotropic medication and mental health service use before and after diagnosis. Multivariable adjusted interrupted time series analyses (ITSA) were conducted to uncover temporal patterns.ResultsFifteen percent of men commenced psychotropic medications and 6.4% sought out mental health services for the first time after diagnosis. Psychotropic medication use rose from 34.5% 5 years before to 40.3% 5 years after diagnosis, including an increase in use of antidepressants (from 20.7% to 26.0%) and anxiolytics (from 11.3% to 12.8%). Mental health service use increased from 10.2% to 12.1%, with the increase mostly being general practice mental health visits (from 7.8% to 10.6%). Multivariable ITSA indicated a significant rise in medication and service utilisation immediately before and in the first 2 years following prostate cancer diagnosis.ConclusionThere is a clear increase in psychotropic medication use and mental health service use around the time of prostate cancer diagnosis. Mental health outcomes of men with prostate cancer may be improved with early mental health screening, particularly during the diagnosis process, to enable early intervention.