bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Substituting Dietary Soybean for Air‐Classified Sunflower (Helianthus annuus L.) Meal on Egg Production and Steroid Hormones in Early‐Phase Laying Hens
Authors: Laudadio V , Ceci E , Nahashon SN , Introna M , Lastella NMB , Tufarelli V 
Published: October 2013

ContentsSoybean meal (SBM) is the most widely and expensive protein source used in the formulation of poultry diets; however, when the price of SBM increases, poultry nutritionists seek alternative sources that are more economical in formulating least‐cost rations. This research aimed to evaluate the effects of dietary air‐classified sunflower meal (SFM) on some productive parameters and plasma steroid hormones in laying hens. In this trial, 20‐week‐old laying hens (ISA Brown strain) in the early phase of production were randomly assigned to two groups and fed wheat middlings‐based diets containing soybean (135 g/kg; 48% CP) or air‐classified SFM (160 g/kg; 41% CP) as the main protein source. Laying performance, egg size and feed conversion ratio were evaluated for 10 week. Plasma steroid hormones (progesterone and oestradiol) in the hens were quantified weekly. Substituting SBM with air‐classified SFM did not change (p > 0.05) the hens' growth performance, whereas feed consumption and efficiency were positively influenced (p < 0.05) by SFM treatment. Egg production rate was improved in hens fed the SFM diet (p < 0.05), as well as the percentage of medium‐size eggs that was higher for SFM treatment (p < 0.05). Steroid hormones levels were affected by dietary treatment (p < 0.01). From our findings, it could be effective to include air‐classified SFM in early‐phase laying hen diets as an alternative protein source substituting SBM, without negative influence on productive performance and egg traits, reducing also the production costs.
Association of BK viremia with human leukocyte antigen mismatches and acute rejection, but not with type of calcineurin inhibitor
Authors: Hässig A. , Roos M. , Etter A. , Bossart W. , Müller N. , Schiesser M. , Wüthrich R.P. , Fehr T. 
Published: October 2013

AbstractIntroductionBK viremia and polyomavirus‐associated nephropathy (PVN) represent a significant problem after kidney transplantation. Both are associated with intensified immunosuppression, but other risk factors and the impact of a screening program on outcome are incompletely understood.MethodsHere, we report on the short‐ and long‐term outcome of a cohort of patients, who were transplanted in 2006/2007 and included in a newly introduced systematic 3‐monthly screening for BK viremia at the University Hospital Zurich. In patients testing positive for BK viremia, screening frequency was intensified and immunosuppression reduced. Patients with suspected PVN underwent transplant biopsy.ResultsAmong 152 included patients, 49 (32%) tested positive for BK viremia, but only 8 developed biopsy‐proven PVN. BK viremia had a significant impact on estimated glomerular filtration rate and proteinuria in the first 2 years. Acute rejection episodes and the number of human leukocyte antigen (HLA) mismatches were the strongest independent predictors of BK viremia in a multiple logistic model. In contrast, no particular immunosuppressive agent or regimen was associated with enhanced risk.ConclusionTaken together, systematic BK viremia screening led to detection of a high percentage of viremic patients. With adjustment of immunosuppression, an excellent outcome was achieved. The independent association of HLA mismatches with BK viremia suggests impaired polyomavirus immunosurveillance in highly mismatched allografts.
Persistent parvovirus B19 infection resulting in red cell aplasia after allogeneic hematopoietic stem cell transplantation
Authors: Koda Y. , Mori T. , Kato J. , Kohashi S. , Kikuchi T. , Mitsuhashi T. , Murata M. , Uemura T. , Handa M. , Okamoto S. 
Published: October 2013

AbstractPersistent parvovirus B19 (PVB) infection has been reported sporadically in immunocompromised patients including hematopoietic stem cell and solid organ transplant recipients. However, the pathogenesis of persistent infection has yet to be fully elucidated. We report here a patient with multiple myeloma developing red cell aplasia during the hematopoietic recovery after allogeneic hematopoietic stem cell transplantation (HSCT) caused by PVB. The patient had already had PVB viremia before transplantation and remained asymptomatic. The route of PVB transmission was considered to be direct contact with the patient's family member with primary PVB infection 1 month before transplantation. Treatment with intravenous immunoglobulin resulted in prompt resolution of anemia. These findings suggest that monitoring of PVB DNA is recommended for patients undergoing HSCT and having contact with individuals with documented PVB infection, even if they are asymptomatic.
Glucose‐6‐phosphate dehydrogenase deficiency in Italian blood donors: prevalence and molecular defect characterization
Authors: Maffi D. , Pasquino M. T. , Mandarino L. , Tortora P. , Girelli G. , Meo D. , Grazzini G. , Caprari P. 
Published: October 2013

BackgroundIn the countries with high G6PD deficiency prevalence, blood donors are not routinely screened for this genetic defect. G6PD deficiency is often asymptomatic, blood donors may be carriers of the deficiency without being aware of it. The aim of the study was to evaluate the prevalence of G6PD deficiency among the Italian blood donors.Design and MethodsFrom October 2009 to April 2011, 3004 blood donors from a large hospital transfusion centre were screened for G6PD deficiency using differential pH‐metry and the characterization of G6PD mutations was performed on G6PD‐deficient subjects. The haematological features of G6PD‐deficient and normal donors were also compared.ResultsThirty‐three subjects (25 men and 8 women) with low G6PD activity were identified, corresponding to 1·1% of the examined blood donor population. The frequencies of class II severe alleles (Mediterranean, Valladolid, Chatham and Cassano) and class III mild alleles (Seattle, A‐ and Neapolis) were 48% and 43%, respectively. The haematological parameters of G6PD‐ donors were within normal range; however, the comparison between normal and G6PD‐ class II donors showed significant differences.ConclusionIn Italy, the presence of blood donors with G6PD deficiency is not a rare event and the class II severe variants are frequent. The identification of G6PD‐deficient donors and the characterization of the molecular variants would prevent the use of G6PD‐deficient RBC units when the haemolytic complications could be relevant especially for high risk patients as premature infants and neonates and patients with sickle cell disease submitted to multiple transfusions.
Identification of Genetic Variants That Affect Histone Modifications in Human Cells
Authors: McVicker Graham , van de Geijn Bryce , Degner Jacob F. , Cain Carolyn E. , Banovich Nicholas E. , Raj Anil , Lewellen Noah , Myrthil Marsha , Gilad Yoav , Pritchard Jonathan K. 
Published: October 2013

DNA DifferencesThe extent to which genetic variation affects an individual's phenotype has been difficult to predict because the majority of variation lies outside the coding regions of genes. Now, three studies examine the extent to which genetic variation affects the chromatin of individuals with diverse ancestry and genetic variation (see the Perspective byFurey and Sethupathy).Kasowskiet al.(p.750, published online 17 October) examined how genetic variation affects differences in chromatin states and their correlation to histone modifications, as well as more general DNA binding factors.Kilpinenet al.(p.744, published online 17 October) document how genetic variation is linked to allelic specificity in transcription factor binding, histone modifications, and transcription.McVickeret al.(p.747, published online 17 October) identified how quantitative trait loci affect histone modifications in Yoruban individuals and established which specific transcription factors affect such modifications.
Draft Genome Sequences of Pseudomonas aeruginosa B3 Strains Isolated from a Cystic Fibrosis Patient Undergoing Antibiotic Chemotherapy
Authors: Marvig Rasmus Lykke , Jochumsen Nicholas , Johansen Helle Krogh , Høiby Niels , Molin Søren , Sommer Morten O. , Jelsbak Lars , Folkesson Anders 
Published: October 2013

ABSTRACT

Pseudomonas aeruginosa
frequently establishes chronic infections in the airways of patients suffering from cystic fibrosis (CF). Here, we report the draft genome sequences of four
P. aeruginosa
B3 strains isolated from a chronically infected CF patient undergoing antibiotic chemotherapy.
Draft Genome Sequences of Burkholderia cenocepacia ET12 Lineage Strains K56-2 and BC7
Authors: Varga John J. , Losada Liliana , Zelazny Adrian M. , Kim Maria , McCorrison Jamison , Brinkac Lauren , Sampaio Elizabeth P. , Greenberg David E. , Singh Indresh , Heiner Cheryl , Ashby Meredith , Nierman William C. , Holland Steven M. , Goldberg Joanna B. 
Published: October 2013

ABSTRACT

The
Burkholderia cepacia
complex (BCC) is a group of closely related bacteria that are responsible for respiratory infections in immunocompromised humans, most notably those with cystic fibrosis (CF). We report the genome sequences for
Burkholderia cenocepacia
ET12 lineage CF isolates K56-2 and BC7.
Hepatitis B in pregnancy
Authors: Dyson Jessica Katharine , Waller Julia , Turley Andrena , Michael Enid , Moses Samuel , Valappil Manoj , Hudson Mark , Bassendine Margaret , McPherson Stuart 
Published: October 2013

ObjectiveVertical transmission of the hepatitis B virus (HBV) is the commonest mode of infection and can be prevented with immunoprophylaxis of the infant and antiviral therapy in the mother. Our aim was to review a cohort of subjects with HBV in pregnancy to determine the prevalence of active disease or high HBV-DNA levels that required treatment to prevent transmission, and to review the management of mothers and infants.MethodsA retrospective case-note review was conducted of all the HBV-infected pregnant women and their infants who attended the Newcastle obstetric services from 2007 to 2011.ResultsThere were 113 pregnancies in 81 women (median age 28 years; 15% hepatitis B e antigen (HBeAg) positive) during 2007–11. 71% of mothers were first diagnosed with HBV during pregnancy. The mothers were born in 28 different countries. 69% of mothers had an HBV-DNA level less than 2000 IU/mL and 13% had HBV-DNA levels greater than 1.0×107 IU/mL so would be eligible for antiviral therapy to prevent transmission to the infant. 9% had active eAg-positive HBV and 3% had active eAg-negative HBV requiring treatment. All infants born to HBeAg-positive mothers received hepatitis B immunoglobulin (HBIG) appropriately and 76% of infants received a full HBV vaccination course. One infant born to an HBeAg-negative mother was hepatitis B surface antigen positive 1 year post-delivery.ConclusionsOne in six women had active HBV requiring treatment or high HBV-DNA levels that would benefit from antiviral treatment to reduce the transmission risk. HBIG was administered appropriately but completion of the vaccination course was suboptimal.
A Heterodimeric Fc-Based Bispecific Antibody Simultaneously Targeting VEGFR-2 and Met Exhibits Potent Antitumor Activity
Authors: Choi Hye-Ji , Kim Ye-Jin , Lee Sangho , Kim Yong-Sung 
Published: October 2013

Abstract
Heterodimeric Fc designed by engineering the CH3 homodimeric interface of immunoglobulin G1 serves as an attractive scaffold for the generation of bispecific antibodies (bsAb) due to the favorable properties of the Fc region. In this study, we describe a heterodimeric Fc generated by substituting the conserved electrostatic interactions at the CH3 core interface with asymmetric hydrophobic interactions and introducing asymmetric, long-range electrostatic interactions at the rim of the CH3 interface. Coexpression of Fc proteins carrying the combined CH3 variant pairs in HEK293F cells produced the heterodimer, which was purified with more than 90% yield, and retained wild-type Fc biophysical properties. The heterodimeric Fc was exploited to generate a bsAb simultaneously targeting both the Met receptor tyrosine kinase and the VEGF receptor 2 (VEGFR-2), with two respective antigen-specific, single-chain variable fragments (scFv) into the N-terminus. The Met × VEGFR-2 bsAb bound concurrently to the two target antigens, efficiently inhibited the downstream signaling and tube formation stimulated by the two receptors in human endothelial cells, and exhibited more potent antitumor efficacy in MKN45 human gastric cancer xenograft models than both the parent monospecific antibody alone. Collectively, based on the newly designed heterodimeric Fc-based bsAb, our results provide the therapeutic potential of bsAb targeting both Met and VEGFR-2 simultaneously for the treatment of human cancers. Mol Cancer Ther; 12(12); 2748–59. ©2013 AACR.
Hedgehog-Dependent Regulation of Angiogenesis and Myogenesis Is Impaired in Aged Mice
Authors: Renault Marie-Ange , Robbesyn Fanny , Chapouly Candice , Yao Qinyu , Vandierdonck Soizic , Reynaud Annabel , Belloc Isabelle , Traiffort Elisabeth , Ruat Martial , Desgranges Claude , Gadeau Alain-Pierre 
Published: October 2013

Objective—
The purpose of this study is to further document alteration of signal transduction pathways, more particularly of hedgehog (Hh) signaling, causing impaired ischemic muscle repair in old mice.

Approach and Results—
We used 12-week-old (young mice) and 20- to 24-month-old C57BL/6 mice (old mice) to investigate the activity of Hh signaling in the setting of hindlimb ischemia–induced angiogenesis and skeletal muscle repair. In this model, delayed ischemic muscle repair observed in old mice was associated with an impaired upregulation of Gli1. Sonic Hh expression was not different in old mice compared with young mice, whereas desert Hh (Dhh) expression was downregulated in the skeletal muscle of old mice both in healthy and ischemic conditions. The rescue of Dhh expression by gene therapy in old mice promoted ischemia-induced angiogenesis and increased nerve density; nevertheless, it failed to promote myogenesis or to increase Gli1 mRNA expression. After further investigation, we found that, in addition to Dhh, smoothened expression was significantly downregulated in old mice. We used smoothened haploinsufficient mice to demonstrate that smoothened knockdown by 50% is sufficient to impair activation of Hh signaling and ischemia-induced muscle repair.

Conclusions—
The present study demonstrates that Hh signaling is impaired in aged mice because of Dhh and smoothened downregulation. Moreover, it shows that hegdehog-dependent regulation of angiogenesis and myogenesis involves distinct mechanisms.
Characterization of Catheter–Tissue Contact Force During Epicardial Radiofrequency Ablation in an Ovine Model
Authors: Wong Michael C.G. , Edwards Glenn , Spence Steven J. , Kalman Jonathan M. , Kumar Saurabh , Joseph Stephen A. , Morton Joseph B. 
Published: October 2013

Background—
Contact force (CF) during radiofrequency ablation (RFA) is an important determinant of endocardial lesion size with limited data on epicardial RFA and CF. We evaluated CF characteristics using irrigated RFA on the epicardium in an ovine model.

Methods and Results—

In 12 sheep, a 7-F irrigated RFA catheter with CF sensor was introduced via a pericardial incision onto/in parallel with ventricular epicardium. RFA (30 W per 30 second duration) was applied at 5
g
, 10
g
, 20
g
, 40
g
, and 70
g
: (1) over left and right ventricular myocardium with or without fat, (2) either directly over or adjacent to a coronary artery, or directly over the phrenic nerve. Force–time integral, lesion dimensions, and coronary artery/phrenic nerve injury were recorded. Lesion size, volume, and force–time integral progressively increased with higher CF (
P
<0.05). Steam pops occurred with high CF. Epicardial fat had an attenuating effect on RF penetration into myocardium (
P
<0.05); however, myocardial RF lesions could be created at sites with >3.5 mm epicardial fat. At sites with epicardial fat, each 10
g
increment in CF led to a 0.6 mm increase in lesion depth, whereas each 1 mm of fat reduced lesion depth into underlying myocardium by 0.7 mm. Extent of acute coronary injury with direct and indirect RFA and phrenic nerve palsy occurrence was proportional to CF.

Conclusions—
CF is a determinant of epicardial RF lesion size, steam pops, acute coronary artery injury, and phrenic nerve injury. Although epicardial fat limits lesion size, RFA with high CF can produce small myocardial RF lesions at sites of thick epicardial fat.
Chronic Akt1 Deficiency Attenuates Adverse Remodeling and Enhances Angiogenesis After Myocardial Infarction
Authors: Vandoorne Katrien , Vandsburger Moriel H. , Raz Tal , Shalev Moran , Weisinger Karen , Biton Inbal , Brumfeld Vlad , Raanan Calanit , Nevo Nava , Eilam Raya , Hemmings Brian A. , Tzahor Eldad , Harmelin Alon , Gepstein Lior , Neeman Michal 
Published: October 2013

Background—
Akt1 is a key signaling molecule in multiple cell types, including endothelial cells. Accordingly, Akt1 was proposed as a therapeutic target for ischemic injury in the context of myocardial infarction (MI). The aim of this study was to use multimodal in vivo imaging to investigate the impact of systemic Akt1 deficiency on cardiac function and angiogenesis before and after MI.

Methods and Results—

In vivo cardiac MRI was performed before and at days 1, 8, 15, and 29 to 30 after MI induction for wild-type, heterozygous, and Akt1-deficient mice. Noninfarcted hearts were imaged using ex vivo stereomicroscopy and microcomputed tomography. Histological examination was performed for noninfarcted hearts and for hearts at days 8 and 29 to 30 after MI. MRI revealed mildly decreased baseline cardiac function in Akt1 null mice, whereas ex vivo stereomicroscopy and microcomputed tomography revealed substantially reduced coronary macrovasculature. After MI, Akt1
–/–
mice demonstrated significantly attenuated ventricular remodeling and a smaller decrease in ejection fraction. At 8 days after MI, a larger functional capillary network at the remote and border zone, accompanied by reduced scar extension, preserved cardiac function, and enhanced border zone wall thickening, was observed in Akt1
–/–
mice when compared with littermate controls.

Conclusions—
Using multimodal imaging to probe the role of Akt1 in cardiac function and remodeling after MI, this study revealed reduced adverse remodeling in Akt1-deficient mice after MI. Augmented myocardial angiogenesis coupled with a more functional myocardial capillary network may facilitate revascularization and therefore be responsible for preservation of infarcted myocardium.
Australian Public’s Awareness of Stroke Warning Signs Improves After National Multimedia Campaigns
Authors: Bray Janet E. , Johnson Roslyn , Trobbiani Kym , Mosley Ian , Lalor Erin , Cadilhac Dominique 
Published: October 2013

Background and Purpose—
The aim of this study was to examine the reach and impact of the National Stroke Foundation (NSF) multimedia stroke warning sign campaigns across Australia.

Methods—
A total of 12 439 surveys were performed across 6 states during 6 years on random state–weighted samples of Australians ≥40 years old.

Results—

Awareness of stroke advertising increased 31% to 50% between 2004 and 2010 (
P
<0.001), as did the unprompted recall of ≥2 most common stroke warning signs 20% to 53% (
P
<0.001). Awareness of stroke advertising was independently associated with recalling ≥2 common signs (adjusted odds ratio=1.88, 95% confidence interval [1.74–2.04];
P
<0.001). Awareness was not greater in respondents with previous stroke or risk factors, except atrial fibrillation.

Conclusions—
The Australian public’s awareness of stroke warning signs has improved since commencement of the NSF campaigns commensurate with greater awareness of stroke advertising. Public education efforts are worthwhile, and future efforts should focus on groups identified with low awareness or those at high risk of stroke.
Effects of MLC601 on Early Vascular Events in Patients After Stroke
Authors: Chen Christopher L.H. , Venketasubramanian Narayanaswamy , Lee Chun Fan , Wong K.S. Lawrence , Bousser Marie-Germaine ,   CHIMES Study Investigators,   Philippines, Navarro Jose C. , Gan Herminigildo H. , Lao Annabelle Y. , Baroque Alejandro C. , Lokin Johnny , Hiyadan John Harold B. , Sarfati Ma. Socorro , Fangonillo Randolph John , Ambasing Neil , Chua Carlos L. , San Jose Ma. Cristina , Advincula Joel M. , Berame Eli John , Canete Maria Teresa ,   Singapore, Venketasubramanian Narayanaswamy , Young Sherry H.Y. , Mamauag Marlie Jane , Tay San San , Pande Shrikant , Thirugnanam Umapathi , Singh Rajinder , Chang Hui Meng , De Silva Deidre Anne , Chan Bernard P.L. , Sharma Vijay , Luen Teoh Hock ,   Thailand, Poungvarin Niphon , Muengtaweepongsa Sombat , Towanabut Somchai , Suwanwela Nijasri C. , Chotickanuchit Songkram , Chankrachang Siwaporn , Nitinun Samart ,   Sri Lanka, de Silva H. Asita , Ranawake Udaya , Wijekoon Nirmala ,   Hong Kong, Wong K.S. Lawrence ,   Malaysia, Eow Gaik Bee 
Published: October 2013

Background and Purpose—
Early vascular events are an important cause of morbidity and mortality in the first 3 months after a stroke. We aimed to investigate the effects of MLC601 on the occurrence of early vascular events within 3 months of stroke onset.

Methods—
Post hoc analysis was performed on data from subjects included in the CHInese Medicine Neuroaid Efficacy on Stroke recovery (CHIMES) study, a randomized, placebo-controlled, double-blinded trial that compared MLC601 with placebo in 1099 subjects with ischemic stroke of intermediate severity in the preceding 72 hours. Early vascular events were defined as a composite of recurrent stroke, acute coronary syndrome, and vascular death occurring within 3 months of stroke onset.

Results—

The frequency of early vascular events during the 3-month follow-up was significantly less in the MLC601 group than in the placebo group (16 [2.9%] versus 31 events [5.6%]; risk difference=−2.7%; 95% confidence interval, −5.1% to −0.4%;
P
=0.025) without an increase in nonvascular deaths. Kaplan–Meier survival analysis showed a difference in the risk of vascular outcomes between the 2 groups as early as the first month after stroke (Log-rank
P
=0.024; hazard ratio, 0.51; 95% confidence interval, 0.28–0.93).

Conclusions—
Treatment with MLC601 was associated with reduced early vascular events among subjects in the CHIMES study. The mechanisms for this effect require further study.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT00554723.