bluuebunny/crossref_metadata_2025_split huggingface dataset:
Playing with deviance: Typicality assessments of ingroup members as a strategy of outgroup approach
Authors: Teixeira Cátia P. , Demoulin Stéphanie , Yzerbyt Vincent Y. 
Published: December 2012

AbstractIn two experiments, we analyzed the use of intra‐group differentiation between normative and deviant members as an identity mobilization strategy in intergroup negotiations. Because ingroup members sometimes try to obtain the support of outgroup audiences to attain their goals, we propose that in intergroup negotiations, people attempt to minimize the distinction between the parties involved by changing the appraisal of deviance and including deviant members in the ingroup's prototype. In line with this hypothesis, differences in the assessment of typicality between normative and deviant targets were reduced in instrumental intergroup negotiation contexts. Furthermore, we explored a boundary condition for this effect and found that such outgroup approach is disrupted when threats taint the intergroup negotiation context. Copyright © 2012 John Wiley & Sons, Ltd.
Selective tyrosine kinase inhibition of insulin-like growth factor-1 receptor inhibits human and mouse breast cancer–induced bone cell activity, bone remodeling, and osteolysis
Authors: Logan John G , Sophocleous Antonia , Marino Silvia , Muir Morwenna , Brunton Valerie G , Idris Aymen I 
Published: December 2012

Abstract
Insulin-like growth factor 1 (IGF-1) plays an important role in both bone metabolism and breast cancer. In this study, we investigated the effects of the novel IGF-1 receptor tyrosine kinase inhibitor cis-3-[3-(4-methyl-piperazin-l-yl)-cyclobutyl]-1-(2-phenyl-quinolin-7-yl)-imidazo[1,5-a]pyrazin-8-ylamine (PQIP) on osteolytic bone disease associated with breast cancer. Human MDA-MB-231 and mouse 4T1 breast cancer cells enhanced osteoclast formation in receptor activator of NF-κB ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) stimulated bone marrow cultures, and these effects were significantly inhibited by PQIP. Functional studies in osteoclasts showed that PQIP inhibited both IGF-1 and conditioned medium–induced osteoclast formation by preventing phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) activation without interfering with RANKL or M-CSF signaling. Treatment of osteoblasts with PQIP significantly inhibited the increase in RANKL/osteoprotegerin (OPG) ratio by IGF-1 and conditioned medium and totally prevented conditioned medium–induced osteoclast formation in osteoblast–bone marrow (BM) cell cocultures, thereby suggesting an inhibitory effect on osteoblast–osteoclast coupling. PQIP also inhibited IGF-1–induced osteoblast differentiation, spreading, migration, and bone nodule formation. Treatment with PQIP significantly reduced MDA-MB-231 conditioned medium–induced osteolytic bone loss in a mouse calvarial organ culture system ex vivo and in adult mice in vivo. Moreover, once daily oral administration of PQIP significantly decreased trabecular bone loss and reduced the size of osteolytic bone lesions following 4T1 intratibial injection in mice. Quantitative histomorphometry showed a significant reduction in bone resorption and formation indices, indicative of a reduced rate of cancer-associated bone turnover. We conclude that inhibition of IGF-1 receptor tyrosine kinase activity by PQIP suppresses breast cancer–induced bone turnover and osteolysis. Therefore, PQIP, and its novel derivatives that are currently in advanced clinical development for the treatment of a number of solid tumors, may be of value in the treatment of osteolytic bone disease associated with breast cancer. © 2013 American Society for Bone and Mineral Research.
MiR‐29 mediates TGFβ1‐induced extracellular matrix synthesis through activation of PI3K‐AKT pathway in human lung fibroblasts
Authors: Yang Tao , Liang Ying , Lin Qinlu , Liu Junwen , Luo Feijun , Li Xinhua , Zhou Hui , Zhuang Sheng , Zhang Hongliang 
Published: December 2012

AbstractTGFβ1 is very important in the synthesis and degradation of extracellular matrix, and also in the mediation of human lung fibroblasts proliferation, and miR‐29 plays an important role in this process. To explore the interactions of miR‐29 family members and TGFβ1, the effects of transforming growth factor TGFβ1 on the expression of miR‐29 and whether miR‐29 is involved in pro‐survival signaling pathways mediated by TGFβ1 were examined in human lung fibroblasts. Treatment of the human embryonic lung fibroblast cell line IMR90 with TGFβ1 caused a decrease in expression of miR‐29a/b/c by real‐time PCR analysis. TGFβ1 stimulation increased cell proliferation, colony formation and up‐regulated expression of COL1A1; transfecting with miR‐29a/b/c mimics reverse TGFβ1‐induced phenotype changes in IMR90 cells. Western blot analyses showed that TGFβ1 treatment unchanged total protein expression levels of PI3K or AKT, but the expression levels of p‐PI3K, p‐AKT, and COL1A1 were increased; and miR‐19a/b/c mimics interfering blocked phosphorylation of PI3K or AKT and decreased expression of COL1A1 after TGFβ1 treatment. The results indicate that TGFβ1 beta uses the PI3k‐Akt pathway in these embryonic fibroblasts and miR29 blocks this activation pathway. It indicates a novel biological function of the PI3K‐Akt pathway in IMR90. Elevated expression of miR‐29 may play an important role in the pathogenesis of diseases related to fibrogenic reactions in human lung fibroblasts. J. Cell. Biochem. 114: 1336–1342, 2013. © 2013 Wiley Periodicals, Inc.
Current techniques in reprogramming cell potency
Authors: Romli Firdaus , Alitheen Noorjahan Banu , Hamid Muhajir , Ismail Ruzila , Nik Abd. Rahman Nik‐Mohd‐Afizan 
Published: December 2012

AbstractThe first successful attempt to reprogram somatic cell into embryonic‐like stem cell was achieved on 2006. Since then, it had sparked a race against time to bring this wonderful invention from bench to bedside but it is not easily achieved due to severe problems in term of epigenetic and genomic. With each problem arise, new technique and protocol will be constructed to try to overcome it. This review addresses the various techniques made available to create iPSC with problems hogging down the technique. J. Cell. Biochem. 114: 1230–1237, 2013. © 2012 Wiley Periodicals, Inc.
Lead (II) removal from aqueous solution by chitosan flake modified with citric acid via crosslinking with glutaraldehyde
Authors: Suc Nguyen Van , Ly Ho Thi Yeu 
Published: December 2012

AbstractBACKGROUNDChitosan flake is a bio‐adsorbent that has been studied for adsorption of lead. However, its adsorption capacity for lead was low. To enhance its adsorption capacity, chitosan flakes were modified with citric acid by crosslinking with glutaraldehyde to supplement the functional groups with high affinity for Pb(II) ions.RESULTSModified chitosan flakes with citric were prepared with maximum capacity for Pb(II) of 101.7 mg g−1 at 303 K, pH 5, and 300 min contact time. The experimental data were used to fit kinetic and isotherm models. The results show that the adsorption of Pb(II) on modified chitosan flake followed a pseudo‐second‐order model, and the rate of adsorption was controlled by the mass transport mechanism and intraparticle diffusion. In an equilibrium study, it was found that the Langmuir and Freundlich isotherm models were appropriate to describe the adsorption process, indicating a chemisorption process of Pb(II) on the modified chitosan flake. The negative value of the free energy (ΔG) and the positive values of the enthalpy (ΔH) and entropy (ΔS) indicated an endothermic and spontaneous adsorption process of lead (II) on citric acid grafted chitosan flakes (C‐Gch).CONCLUSIONChitosan flake modified with citric acid by crosslinking with glutaraldehyde remarkably enhanced the adsorption capacity for Pb(II) ions. This material could be used as an effective adsorbent for the removal of Pb(II) from wastewater and contaminated water sources. © 2012 Society of Chemical Industry
The Role of Rapport in Investigative Interviewing: A Review
Authors: Abbe Allison , Brandon Susan E. 
Published: December 2012

AbstractRapport often appears in training and discussions regarding investigative interviewing, yet very little empirical research has examined rapport systematically in law enforcement or intelligence settings. Using a model of rapport developed from therapeutic settings, we address in this paper the components of rapport and their relevance to investigative interviewing. Rapport can play a facilitating role in supporting the goals of an investigative interview, to include developing a working alliance between interviewer and source, exercising social influence, and educing information from a source. A better understanding of how rapport develops in these contexts and its impact on interview outcomes would enhance the effectiveness of investigative interviewing. Research on rapport in the investigative interview would enhance our understanding of the interpersonal dynamics in these situations. We identify several gaps that such research should address, including the relationship between rapport and social influence and the development of rapport in multiparty interactions. Copyright © 2012 John Wiley & Sons, Ltd.
Meta‐analysis of diffusion‐weighted MRI in the differential diagnosis of lung lesions
Authors: Chen Lihua , Zhang Jiuquan , Bao Jing , Zhang Lin , Hu Xiaofei , Xia Yunbao , Wang Jian 
Published: December 2012

AbstractPurpose:To perform a meta‐analysis to evaluate the diagnostic performance of the diffusion‐weighted imaging (DWI) technique in differentiating malignant from benign lung lesions.Materials and Methods:Medical and scientific literature databases were searched for studies that assessed the diagnostic performance of DWI in patients suspected of lung cancer who underwent DWI and biopsy. Only studies in the English or Chinese language and published before September 2011 were considered for inclusion. Methodological quality was assessed by the Quality Assessment of Diagnostic Studies (QUADAS) instrument. Homogeneity was explored by the Chi‐square test and inconsistency index. Sensitivities (SEN), specificities, predictive values, diagnostic odds ratio (dOR), and areas under the receiver operator characteristic (ROC) curve were calculated. Potential threshold effect was investigated by using Spearman's correlation coefficient. Publication bias analysis was evaluated by Deeks' asymmetry test.Results:Of 33 eligible studies, 11 were included in the meta‐analysis, comprising 755 malignant and 294 benign lesions. Heterogeneity was found to have arisen primarily from threshold effect. The data points from the Deeks' funnel plot indicated the presence of publication bias. Methodological quality was moderate. The pooled weighted SEN with corresponding 95% confidence interval (CI) was 0.80 (95% CI: 0.76, 0.83), SPE was 0.93 (95% CI: 0.91, 0.95), positive likelihood ratio was 9.24 (95% CI: 3.58, 23.83), negative likelihood ratio was 0.24 (95% CI: 0.19, 0.29), and dOR was 46.14 (95% CI: 27.56, 77.26). The area under the ROC curve was 0.91 (95% CI: 0.89, 0.93).Conclusion:DWI is a noninvasive, nonradiative, and accurate technique for distinguishing between malignant and benign lung lesions. However, large‐scale randomized control trials are necessary to assess its clinical value and to establish standards of DWI for measurement, analysis, and cutoff values of diagnosis. J. Magn. Reson. Imaging 2013;37:1351–1358. © 2013 Wiley Periodicals, Inc.
Prostate cancer: Utility of diffusion‐weighted imaging as a marker of side‐specific risk of extracapsular extension
Authors: Rosenkrantz Andrew B. , Chandarana Hersh , Gilet Anthony , Deng Fang‐Ming , Babb James S. , Melamed Jonathan , Taneja Samir S. 
Published: December 2012

PurposeTo assess the utility of diffusion‐weighted imaging (DWI) findings as an indirect marker of side‐specific risk of extracapsular extension (ECE) of prostate cancer.Materials and MethodsFifty‐one patients underwent 3T magnetic resonance imaging (MRI) before prostatectomy. Radiologists 1 and 2 (4 and 1 years experience) assessed each side for ECE using T2‐weighted imaging (T2WI) and evaluated apparent diffusion coefficient (ADC) maps for the presence of apparent tumor in each lobe and to measure peripheral zone ADC. A uropathologist measured the extent of any ECE.ResultsIn all, 28/102 lobes had ECE, of which 12 measured ≤1 mm, 11 measured >1 mm and ≤2 mm, and five measured >2 mm. Side‐specific accuracies for detection of ECE for readers 1 and 2 were respectively: T2WI 68.6% and 74.5%; presence of apparent tumor on ADC map 66.7% and 60.8%; ADC value 75.5% and 69.6%. For ECE >2 mm, both readers achieved 100% sensitivity based on apparent tumor on ADC map or ADC values and 80% sensitivity using T2WI. For detection of ECE ≤2 mm, sensitivity for all combinations of the three methods and two readers ranged from 58.3%–81.8%, aside from assessment for ECE using T2WI by the less experienced reader, which exhibited sensitivity of 17.4%. Interreader agreement for the presence of ECE was 0.18 using T2WI, 0.37 using apparent tumor on ADC map, and 0.60 using ADC values.ConclusionCompared with T2WI, DWI had comparable accuracy for side‐specific assessment of ECE, greater sensitivity for ECE <2 mm for the less‐experienced radiologist, and greater interreader agreement. J. Magn. Reson. Imaging 2013;38:312–319. © 2012 Wiley Periodicals, Inc.
Wnt pathway regulation by demineralized bone is approximated by both BMP‐2 and TGF‐β1 signaling
Authors: Zhou Shuanhu , Mizuno Shuichi , Glowacki Julie 
Published: December 2012

AbstractAllogeneic demineralized bone is used extensively as a clinical graft material because it has osteo/chondroinductive and osteoconductive properties. Demineralized bone powder (DBP) induces chondrogenic differentiation of human dermal fibroblasts (hDFs) in three‐dimensional collagen cultures, but the initiating mechanisms have not been fully characterized nor has it been shown that bone morphogenetic proteins (BMPs) recapitulate DBP's effects on target cells. Among the many signaling pathways regulated in hDFs by DBP prior to in vitro chondrogenesis, there are changes in Wnts and their receptors that may contribute to DBP actions. This study tests the hypothesis that DBP modulation of Wnt signaling entails both BMP and TGF‐β pathways. We compared the effects of DBP, TGF‐β1, or BMP‐2 on Wnt signaling components in hDFs by Wnt signaling macroarray, RT‐PCR, in situ hybridization, and Western immunoblot analyses. Many effects of DBP on Wnt signaling components were not shared by BMP‐2, and likewise DBP effects on Wnt genes and β‐catenin only partially required the TGF‐β pathway, as shown by selective inhibition of TGF‐β/activin receptor‐like kinase. The analyses revealed that 64% (16/25) of the Wnt signaling components regulated by DBP were regulated similarly by the sum of effects by BMP‐2 and by TGF‐β1. In conclusion, signaling mechanisms of inductive DBP in human dermal fibroblasts involve the modulation of multiple Wnt signals through both BMP and TGF‐β pathways. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 554–560, 2013
Effects of selective paralysis of the supraspinatus muscle using botulinum neurotoxin a in rotator cuff healing in rats
Authors: Ficklscherer Andreas , Hartl Tessa‐Katharina , Scharf Markus , Sievers Birte , Schröder Christian , Milz Stefan , Niethammer Thomas , Pietschmann Matthias F. , Müller Peter E. 
Published: December 2012

AbstractWe hypothesized that a temporary rotator cuff paralysis using botulinum‐neurotoxin A (BoNtA) would lead to an improved tendon‐to‐bone healing after repair of supraspinatus lesions. One hundred sixty Sprague–Dawley rats were randomly assigned to either the BoNtA or the control (saline) group. BoNtA/saline‐solution was injected into the supraspinatus muscle 1 week prior to surgery. A supraspinatus defect was made; we distinguished between a lesion with normal and increased repair load. Furthermore, one subgroup had the operated shoulder immobilized in a cast. Histologic analysis and biomechanical testing followed. Specimens from the BoNtA‐group, which were treated with an increased repair load, showed less cellularity and more organization in the interface tissue compared to the saline control group. In addition, we found that the collagen 1–3 quotient in the BoNtA specimen was significantly (p = 0.0051) higher than in the control group. Ultimate load at failure between the groups was not significantly different (p > 0.05). We did not observe any significant differences between the mobilized and immobilized specimen (p = 0.2079). The study shows that tendon‐to‐bone healing after rotator cuff repair can be altered positively using BoNtA pre‐operatively. Tears with increased repair load seem to benefit the most—at least histologically. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 716–723, 2013
Local insulin therapy affects fracture healing in a rat model
Authors: Park Andrew G. , Paglia David N. , Al‐Zube Loay , Hreha Jeremy , Vaidya Swaroopa , Breitbart Eric , Benevenia Joseph , O'Connor J. Patrick , Lin Sheldon S. 
Published: December 2012

AbstractA significant number of lower extremity fractures result in mal‐union necessitating effective treatments to restore ambulation. Prior research in diabetic animal fracture models demonstrated improved healing following local insulin application to the fracture site and indicated that local insulin therapy can aid bone regeneration, at least within an insulin‐dependent diabetic animal model. This study tested whether local insulin therapy could accelerate femur fracture repair in normal, non‐diabetic rats. High (20 units) and low (10 units) doses of insulin were delivered in a calcium sulfate carrier which provided sustained release of the exogenous insulin for 7 days after fracture. Histomorphometry, radiographic scoring, and torsional mechanical testing were used to measure fracture healing. The fracture calluses from rats treated with high‐dose insulin had significantly more cartilage than untreated rats after 7 and 14 days of healing. After 4 weeks of healing, femurs from rats treated with low‐dose insulin had significantly higher radiographic scores and mechanical strength (p < 0.05), compared to the no treatment control groups. The results of this study suggest that locally delivered insulin is a potential therapeutic agent for treating bone fractures. Further studies are necessary, such as large animal proof of concepts, prior to the clinical use of insulin for bone fracture treatment. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 776–782, 2013
Effects of local insulin delivery on subperiosteal angiogenesis and mineralized tissue formation during fracture healing
Authors: Paglia David N. , Wey Aaron , Breitbart Eric A. , Faiwiszewski Jonathan , Mehta Siddhant K. , Al‐Zube Loay , Vaidya Swaroopa , Cottrell Jessica A. , Graves Dana , Benevenia Joseph , O'Connor J. Patrick , Lin Sheldon S. 
Published: December 2012

AbstractLocal insulin delivery has been shown to improve osseous healing in diabetic animals. The purpose of this study was to quantify the effects of local intramedullary delivery of saline or Ultralente insulin (UL) on various fracture healing parameters using an in vivo non‐diabetic BB Wistar rat model. Quantitation of local insulin levels showed a rapid release of insulin from the fractured femora, demonstrating complete release at 2 days. RT‐PCR analysis revealed that the expression of early osteogenic markers (Col1α2, osteopontin) was significantly enhanced with UL treatment when compared with saline controls (p < 0.05). Significant differences in VEGF + cells and vascularity were evident between the treatment and control groups at day 7 (p < 0.05). At day 21, histomorphometric analysis demonstrated a significant increase in percent mineralized tissue in the UL‐treated animals compared with controls (p < 0.05), particularly within the subperiosteal region of the fracture callus. Mechanical testing at 4 weeks showed significantly greater mechanical strength for UL‐treated animals (p < 0.05), but healing in control animals caught up at 6 weeks post‐fracture. These results suggest that the primary osteogenic effect of UL during the early stages of fracture healing (1–3 weeks) is through an increase in osteogenic gene expression, subperiosteal angiogenesis, and mineralized tissue formation. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 783–791, 2013
Degradable Poly(amidoamine) Hydrogels as Scaffolds for In Vitro Culturing of Peripheral Nervous System Cells
Authors: Mauro Nicolò , Manfredi Amedea , Ranucci Elisabetta , Procacci Patrizia , Laus Michele , Antonioli Diego , Mantovani Cristina , Magnaghi Valerio , Ferruti Paolo 
Published: December 2012

AbstractThis paper reports on the synthesis and physico‐chemical, mechanical, and biological characterization of two sets of poly(amidoamine) (PAA) hydrogels with potential as scaffolds for in vivo peripheral nerve regeneration. They are obtained by polyaddition of piperazine with N,N′‐methylenebis(acrylamide) or 1,4‐bis(acryloyl)piperazine with 1,2‐diaminoethane as cross‐linking agent and exhibit a combination of relevant properties, such as mechanical strength, biocompatibility, biodegradability, ability to induce adhesion and proliferation of Schwann cells (SCs) preserving their viability. Moreover, the most promising hydrogels, that is those deriving from 1,4‐bis(acryloyl)piperazine, allow the in vitro growth of the sensitive neurons of the dorsal root ganglia, thus getting around a critical point in the design of conduits for nerve regeneration. magnified image
Matrimid Aerogels by Temperature‐Controlled, Solution‐Based Crosslinking
Authors: Kuiper Stefan , Embrechts Anika , Every Hayley A. , de Vries Tjerk , de Smet Louis C. P. M. 
Published: December 2012

AbstractThis paper reports on a temperature‐controlled, solution‐based method to prepare diamine crosslinked Matrimid aerogels. Addition of a diamine to a preheated polymer solution resulted in a well‐dispersed solution, allowing formation of a homogeneous gel upon cooling. The gels (studied by FTIR and AFM) were dried by solvent extraction with supercritical CO2. The resulting aerogels showed surface areas of approximately 150 m2 · g−1 and porosities of 0.66–0.69 mL · g−1 with polymer domains and pore sizes of tens of nanometers. A room temperature‐prepared, inhomogeneous aerogel gave approximately 250 m2 · g−1 and 0.31 mL · g−1 with meso‐ and micropores. SEM images of the aerogels show similar surface features as AFM images of the Matrimid solvent gels. magnified image
Transient computational fluid dynamics modeling of pervaporation separation of aromatic/aliphatic hydrocarbon mixtures using polymer composite membrane
Authors: Rezakazemi Mashallah , Iravaninia Mona , Shirazian Saeed , Mohammadi Toraj 
Published: December 2012

AbstractPervaporation (PV) separation of toluene/n‐heptane mixtures was studied experimentally and theoretically by means of a molecular surface engineering (MSE) polymer composite membrane. A comprehensive mathematical model was developed to predict unsteady state transport of toluene and n‐heptane (nC7) through the membrane. Conservation equations including continuity, and heat transfer equations were solved using finite element method (FEM). Computational fluid dynamics (CFD) technique was applied to solve the model equations. The model was then verified with PV experimental data. The simulation results were in good agreement with the experimental data. The simulation results revealed that the proposed model could provide a general simulation of transport in the PV process. POLYM. ENG. SCI., 2013. © 2012 Society of Plastics Engineers