bluuebunny/crossref_metadata_2025_split huggingface dataset:
Why camera traps fail to detect a semi‐aquatic mammal: Activation devices as possible cause
Authors: Lerone Laura , Carpaneto Giuseppe M. , Loy Anna 
Published: January 2015

ABSTRACTCamera‐trapping represents a powerful tool in wildlife research, particularly when dealing with elusive and rare species such as otters (Lutra spp.). Nevertheless, detection problems arise when trying to detect otters with camera traps. We hypothesized that the temperature difference between the otter emerging from the water and the environment was too low to be detected by the standard passive infrared sensors (PIR). During June–September of 2010 and 2011 in the Sangro River basin in central Italy, we designed and tested a new pressure trigger and compared its effectiveness with that of the standard PIR. Results are encouraging and suggest that the new trigger could be able to detect otters and other semi‐aquatic mammals. © 2015 The Wildlife Society.
Evaluation of Content and Accessibility of Hand Fellowship Websites
Authors: Silvestre Jason , Guzman Javier Z. , Abbatematteo Joseph M. , Chang Benjamin , Levin L. Scott 
Published: January 2015

Background Graduates of general, orthopedic, and plastic surgery residencies utilize web-based resources when applying for hand fellowship training. The purpose of this study was to evaluate the accessibility and content of hand fellowship websites (HFWs). Methods Websites of accredited hand surgery fellowships were eligible for study inclusion. HFWs were evaluated for comprehensiveness in the domains of education and recruitment. Website content was correlated with program characteristics via Fisher exact tests. Results Fifteen plastic, 65 orthopedic, and 1 general surgery hand fellowships were analyzed. Seventy-four hand fellowships maintained an HFW (91 %). HFWs were not found for 3 plastic and 4 orthopedic hand programs (20 versus 6 %, p= 0.118). HFWs provided only half of all analyzed content (54 %—education and 49 %—recruitment). Orthopedic programs had more education content than plastic surgery programs (55 versus 44 %, p=0.030). Programs in the South had more education content than programs in the Northeast (63 versus 47 %, p=0.001), but not more than programs in the West (54 %) or Midwest (55 %). Larger programs with more fellows had greater education content than those with only one fellow (57 versus 49 %, p=0.042). Programs affiliated with top-ranked medical schools had less education content than lower-ranked schools (48 versus 56 %, p=0.045). No differences existed in recruitment content between programs. Conclusions Most hand surgery fellowships lack readily accessible and comprehensive websites. The paucity of online content suggests HFWs are underutilized as educational resources and future opportunity may exist to optimize these tools.
One-dimensional capillary jumps
Authors: Argentina M. , Cohen A. , Bouret Y. , Fraysse N. , Raufaste C. 
Published: January 2015

AbstractIn flows where the ratio of inertia to gravity varies strongly, large variations in the fluid thickness appear and hydraulic jumps arise, as depicted by Rayleigh. We report a new family of hydraulic jumps, where the capillary effects dominate the gravitational acceleration. The Bond number – which measures the importance of gravitational body forces compared to surface tension – must be small in order to observe such objects using capillarity as a driving force. For water, the typical length should be smaller than 3 mm. Nevertheless, for such small scales, solid boundaries induce viscous stresses, which dominate inertia, and capillary jumps should not be described by the inertial shock wave theory that one would deduce from Bélanger or Rayleigh for hydraulic jumps. In order to get rid of viscous shears, we consider Plateau borders, which are the microchannels defined by the merging of three films inside liquid foams, and we show that capillary jumps propagate along these deformable conduits. We derive a simple model that predicts the velocity, geometry and shape of such fronts. A strong analogy with Rayleigh’s description is pointed out. In addition, we carried out experiments on a single Plateau border generated with soap films to observe and characterize these capillary jumps. Our theoretical predictions agree remarkably well with the experimental measurements.
Effect of enclosure height on the structure and stability of shear layers induced by differential rotation
Authors: Vo Tony , Montabone Luca , Sheard Gregory J. 
Published: January 2015

AbstractThe structure and stability of Stewartson shear layers with different heights are investigated numerically via axisymmetric simulation and linear stability analysis, and a validation of the quasi-two-dimensional model is performed. The shear layers are generated in a rotating cylindrical tank with circular disks located at the lid and base imposing a differential rotation. The axisymmetric model captures both the thick and thin nested Stewartson layers, which are scaled by the Ekman number ($\mathit{E}\,$) as $\mathit{E}\,^{1/4}$ and $\mathit{E}\,^{1/3}$ respectively. In contrast, the quasi-two-dimensional model only captures the $\mathit{E}\,^{1/4}$ layer as the axial velocity required to invoke the $\mathit{E}\,^{1/3}$ layer is excluded. A direct comparison between the axisymmetric base flows and their linear stability in these two models is examined here for the first time. The base flows of the two models exhibit similar flow features at low Rossby numbers ($\mathit{Ro}$), with differences evident at larger $\mathit{Ro}$ where depth-dependent features are revealed by the axisymmetric model. Despite this, the quasi-two-dimensional model demonstrates excellent agreement with the axisymmetric model in terms of the shear-layer thickness and predicted stability. A study of various aspect ratios reveals that a Reynolds number based on the theoretical Ekman layer thickness is able to describe the transition of a base flow that is reflectively symmetric about the mid-plane to a symmetry-broken state. Additionally, the shear-layer thicknesses scale closely to the expected ${\it\delta}_{vel}\propto A\mathit{E}\,^{1/4}$ and ${\it\delta}_{vort}\propto A\mathit{E}\,^{1/3}$ for shear layers that are not affected by the confinement ($A\mathit{E}\,^{1/4}\lesssim 0.34$ in this system, the ratio of tank height to shear-layer radius). The linear stability analysis reveals that the ratio of Stewartson layer radius to thickness should be greater than $45$ for the stability of the flow to be independent of aspect ratio. Thus, for sufficiently small $A\mathit{E}\,^{1/4}$ and $A\mathit{E}\,^{1/3}$, the flow characteristics remain similar and the linear stability of the flow can be described universally when the azimuthal wavelength is scaled against $A$. The analysis also recovers an asymptotic scaling for the normalized azimuthal wavelength which suggests that ${\it\lambda}_{{\it\theta},c}^{\ast }\propto (|\mathit{Ro}|/\mathit{E}\,^{2})^{-1/5}$ for geometry-independent shear layers at marginal stability.
On bounds and non-existence in the problem of steady waves with vorticity
Authors: Kozlov V. , Kuznetsov N. , Lokharu E. 
Published: January 2015

AbstractFor the problem describing steady gravity waves with vorticity on a two-dimensional unidirectional flow of finite depth the following results are obtained. (i) Bounds are found for the free-surface profile and for Bernoulli’s constant. (ii) If only one parallel shear flow exists for a given value of Bernoulli’s constant, then there are no wave solutions provided the vorticity distribution is subject to a certain condition.
Macrophage peroxisome proliferator-activated receptor γ deficiency delays skin wound healing through impairing apoptotic cell clearance in mice
Authors: Chen H , Shi R , Luo B , Yang X , Qiu L , Xiong J , Jiang M , Liu Y , Zhang Z , Wu Y 
Published: January 2015

AbstractSkin wound macrophages are key regulators of skin repair and their dysfunction causes chronic, non-healing skin wounds. Peroxisome proliferator-activated receptor gamma (PPARγ) regulates pleiotropic functions of macrophages, but its contribution in skin wound healing is poorly defined. We observed that macrophage PPARγ expression was upregulated during skin wound healing. Furthermore, macrophage PPARγ deficiency (PPARγ-knock out (KO)) mice exhibited impaired skin wound healing with reduced collagen deposition, angiogenesis and granulation formation. The tumor necrosis factor alpha (TNF-α) expression in wounds of PPARγ-KO mice was significantly increased and local restoration of TNF-α reversed the healing deficit in PPARγ-KO mice. Wound macrophages produced higher levels of TNF-α in PPARγ-KO mice compared with control. In vitro, the higher production of TNF-α by PPARγ-KO macrophages was associated with impaired apoptotic cell clearance. Correspondingly, increased apoptotic cell accumulation was found in skin wound of PPARγ-KO mice. Mechanically, peritoneal and skin wound macrophages expressed lower levels of various phagocytosis-related molecules. In addition, PPARγ agonist accelerated wound healing and reduced local TNF-α expression and wound apoptotic cells accumulation in wild type but not PPARγ-KO mice. Therefore, PPARγ has a pivotal role in controlling wound macrophage clearance of apoptotic cells to ensure efficient skin wound healing, suggesting a potential new therapeutic target for skin wound healing.
Targeting heat-shock protein 90 with ganetespib for molecularly targeted therapy of gastric cancer
Authors: Liu H , Lu J , Hua Y , Zhang P , Liang Z , Ruan L , Lian C , Shi H , Chen K , Tu Z 
Published: January 2015

AbstractGastric cancer (GC) remains the fifth most common cancer worldwide. Heat-shock protein 90 (HSP90) has become an attractive therapeutic target in treating cancers, because of its abnormally high expression in cancers. Several successful cases of HSP90 inhibitors capable of inhibiting GC inspired us to try ganetespib, a clinically promising and actively investigated second-generation HSP90 inhibitor in GC treatment. In our study, we show that ganetespib markedly reduced the growth of MGC-803 and also significantly inhibited the growth of SGC-7901 and MKN-28 in a dose-dependent manner. It induced G2/M cell-cycle arrest and apoptosis in all three cell lines, together with the related markers affected significantly. Mechanistically, ganetespib caused pronounced decrease of expression of classic HSP90 client proteins. Specifically, it greatly affected epidermal growth factor receptor (EGFR) signaling cascades by markedly decreasing the levels of total EGFR and EGFR on cell membranes. EGFR knockdown also induced cell-cycle arrest and apoptosis accompanied with a decrease of several EGFR downstream proteins. These results strongly support that EGFR signaling greatly contributes to the ganetespib inhibitory effects. Besides, we found that the responses of GC cell lines to ganetespib correlated well with their EGFR expression levels: MGC-803, as well as AGS and BGC-803, with higher EGFR expression responded to ganetespib better, whereas SGC-7901 and MKN-28 with lower EGFR levels were much less sensitive to ganetespib. Although SGC-7901 and MKN-28 were not very sensitive to ganetespib, ganetespib worked synergistically with radiation and cisplatin in killing them. Importantly, ganetespib significantly inhibited the growth of xenograft tumorsin vivoas a single agent or in combination with cisplatin. Results of hematoxylin/eosin staining, TUNEL (terminal deoxynucleotidyl transferase dUTP nick-end labeling) assays, and immunohistochemistry staining of phosphorylated cyclin-dependent kinase 1 (pCDK1), EGFR and Ki-67 revealed significant differences in ganetespib-treated tumors. Collectively, our data suggest that ganetespib, as a new potent treatment option, can be used for the molecularly targeted therapy of GC patients according to their expression profiles of EGFR.
Germ cell-specific Atg7 knockout results in primary ovarian insufficiency in female mice
Authors: Song Z-H , Yu H-Y , Wang P , Mao G-K , Liu W-X , Li M-N , Wang H-N , Shang Y-L , Liu C , Xu Z-L , Sun Q-Y , Li W 
Published: January 2015

AbstractPrimary ovarian insufficiency (POI) is a common cause of infertility in around 1–2% of women aged <40 years. However, the mechanisms that cause POI are still poorly understood. Here we showed that germ cell-specific knockout of an essential autophagy induction gene Atg7 led to subfertility in female mice. The subfertility of Atg7 deletion females was caused by severe ovarian follicle loss, which is very similar to human POI patients. Further investigation revealed that germ cell-specific Atg7 knockout resulted in germ cell over-loss at the neonatal transition period. In addition, our in vitro studies also demonstrated that autophagy could protect oocytes from over-loss by apoptosis in neonatal ovaries under the starvation condition. Taken together, our results uncover a new role for autophagy in the regulation of ovarian primordial follicle reservation and hint that autophagy-related genes might be potential pathogenic genes to POI of women.
Glutathione transferases P1/P2 regulate the timing of signaling pathway activations and cell cycle progression during mouse liver regeneration
Authors: Pajaud J , Ribault C , Ben Mosbah I , Rauch C , Henderson C , Bellaud P , Aninat C , Loyer P , Morel F , Corlu A 
Published: January 2015

AbstractGlutathione transferases (GST) are phase II enzymes catalyzing the detoxification of endogenous noxious compounds and xenobiotics. They also regulate phosphorylation activities of MAPKinases in a catalytic-independent manner. Previous studies have demonstrated the regulation of JNK-dependent pathway by GSTP1/2. Considering the crucial role of JNK in the early steps of the hepatocyte cell cycle, we sought to determine whether GSTP1/2 were essential for hepatocyte proliferation following partial hepatectomy (PH). Using a conventional double knockout mouse model for the Gstp1 and Gstp2 genes, we found that the lack of GSTP1/P2 reduced the rate of DNA replication and mitotic index during the first wave of hepatocyte proliferation. The lowered proliferation was associated with the decrease in TNFalpha and IL-6 plasma concentrations, reduced hepatic HGF expression and delayed and/or altered activation of STAT3, JNK and ERK1/2 signaling pathways. In addition, the expression and/or activation of cell cycle regulators such as Cyclin D1, CDK4, E2F1 and MCM7 was postponed demonstrating that the absence of GSTP1/2 delayed the entry into and progression through the G1 phase of the cell cycle and impaired the synchrony of proliferation in hepatocytes following PH. Furthermore, while JNK and its downstream targets c-Jun and ATF2 were activated during the early steps of the liver regeneration in wild-type animals, the constitutively active JNK found in the quiescent liver of Gstp1/2 knockout mice underwent a decrease in its activity after PH. Transient induction of antioxidant enzymes and nitric oxide synthase were also delayed or repressed during the regenerative response. Altogether our results demonstrate that GSTP1/2 are a critical regulators of hepatocyte proliferation in the initial phases of liver regeneration.
Dose-dependent effects of selenite (Se4+) on arsenite (As3+)-induced apoptosis and differentiation in acute promyelocytic leukemia cells
Authors: Wang S , Geng Z , Shi N , Li X , Wang Z 
Published: January 2015

AbstractTo enhance the therapeutic effects and decrease the adverse effects of arsenic on the treatment of acute promyelocytic leukemia, we investigated the co-effects of selenite (Se4+) and arsenite (As3+) on the apoptosis and differentiation of NB4 cells and primary APL cells. A 1.0-μM concentration of Se4+ prevented the cells from undergoing As3+-induced apoptosis by inhibiting As3+ uptake, eliminating As3+-generated reactive oxygen species, and repressing the mitochondria-mediated intrinsic apoptosis pathway. However, 4.0 μM Se4+ exerted synergistic effects with As3+ on cell apoptosis by promoting As3+ uptake, downregulating nuclear factor-кB, and activating caspase-3. In addition to apoptosis, 1.0 and 3.2 μM Se4+ showed contrasting effects on As3+-induced differentiation in NB4 cells and primary APL cells. The 3.2 μM Se4+ enhanced As3+-induced differentiation by promoting the degradation of promyelocytic leukemia protein–retinoic acid receptor-α (PML–RARα) oncoprotein, but 1.0 μM Se4+ did not have this effect. Based on mechanistic studies, Se4+, which is similar to As3+, might bind directly to Zn2+-binding sites of the PML RING domain, thus controlling the fate of PML–RARα oncoprotein.
The role of hypoxia-inducible factor-2 in digestive system cancers
Authors: Zhao J , Du F , Shen G , Zheng F , Xu B 
Published: January 2015

AbstractHypoxia is an all but ubiquitous phenomenon in cancers. Two known hypoxia-inducible factors (HIFs), HIF-1α and HIF-2α, primarily mediate the transcriptional response to hypoxia. Despite the high homology between HIF-1α and HIF-2α, emerging evidence suggests differences between both molecules in terms of transcriptional targets as well as impact on multiple physiological pathways and tumorigenesis. To date, much progress has been made toward understanding the roles of HIF-2α in digestive system cancers. Indeed, HIF-2α has been shown to regulate multiple aspects of digestive system cancers, including cell proliferation, angiogenesis and apoptosis, metabolism, metastasis and resistance to chemotherapy. These findings make HIF-2α a critical regulator of this malignant phenotype. Here we summarize the function of HIF-2 during cancer development as well as its contribution to tumorigenesis in digestive system malignancies.
Reduced cerebrospinal fluid ethanolamine concentration in major depressive disorder
Authors: Ogawa Shintaro , Hattori Kotaro , Sasayama Daimei , Yokota Yuki , Matsumura Ryo , Matsuo Junko , Ota Miho , Hori Hiroaki , Teraishi Toshiya , Yoshida Sumiko , Noda Takamasa , Ohashi Yoshiaki , Sato Hajime , Higuchi Teruhiko , Motohashi Nobutaka , Kunugi Hiroshi 
Published: January 2015

AbstractAmino acids play key roles in the function of the central nervous system and their alterations are implicated in psychiatric disorders. In the search for a biomarker for major depressive disorder (MDD), we used high-performance liquid chromatography to measure amino acids and related molecules in the cerebrospinal fluid (CSF) of 52 patients with MDD (42 depressed and 10 remitted; DSM-IV) and 54 matched controls. Significant differences were found in four amino acid concentrations between the depressed patients and controls. After Bonferroni correction, only ethanolamine (EA) levels remained significantly reduced in depressed patients (nominal P = 0.0000011). A substantial proportion of the depressed patients (40.5%) showed abnormally low CSF EA levels (<12.1 μM) (P = 0.000033; OR = 11.6, 95% CI: 3.1–43.2). When patients with low EA and those with high EA levels were compared, the former had higher scores for overall depression severity (P = 0.0033) and ‘Somatic Anxiety’ symptoms (P = 0.00026). In unmedicated subjects, CSF EA levels showed a significant positive correlation with levels of homovanillic acid (P = 0.0030) and 5-hydroxyindoleacetic acid (P = 0.019). To our knowledge, this is the first study showing that patients with MDD have significantly lower CSF EA concentrations compared with control subjects. CSF EA could be a state-dependent biomarker for a subtype of MDD.
Biological interaction of living cells with COSAN-based synthetic vesicles
Authors: Tarrés Màrius , Canetta Elisabetta , Paul Eleanor , Forbes Jordan , Azzouni Karima , Viñas Clara , Teixidor Francesc , Harwood Adrian J. 
Published: January 2015

AbstractCobaltabisdicarbollide (COSAN) [3,3′-Co(1,2-C2B9H11)2]−, is a complex boron-based anion that has the unusual property of self-assembly into membranes and vesicles. These membranes have similar dimensions to biological membranes found in cells and previously COSAN has been shown to pass through synthetic lipid membranes and those of living cells without causing breakdown of membrane barrier properties. Here, we investigate the interaction of this inorganic membrane system with living cells. We show that COSAN has no immediate effect on cell viability and cells fully recover when COSAN is removed following exposure for hours to days. COSAN elicits a range of cell biological effects, including altered cell morphology, inhibition of cell growth and, in some cases, apoptosis. These observations reveal a new biology at the interface between inorganic, synthetic COSAN membranes and naturally occurring biological membranes.
qPMS9: An Efficient Algorithm for Quorum Planted Motif Search
Authors: Nicolae Marius , Rajasekaran Sanguthevar 
Published: January 2015

AbstractDiscovering patterns in biological sequences is a crucial problem. For example, the identification of patterns in DNA sequences has resulted in the determination of open reading frames, identification of gene promoter elements, intron/exon splicing sites and SH RNAs, location of RNA degradation signals, identification of alternative splicing sites, etc. In protein sequences, patterns have led to domain identification, location of protease cleavage sites, identification of signal peptides, protein interactions, determination of protein degradation elements, identification of protein trafficking elements, discovery of short functional motifs, etc. In this paper we focus on the identification of an important class of patterns, namely, motifs. We study the (ℓ, d) motif search problem or Planted Motif Search (PMS). PMS receives as input n strings and two integers ℓ and d. It returns all sequences M of length ℓ that occur in each input string, where each occurrence differs from M in at most d positions. Another formulation is quorum PMS (qPMS), where the motif appears in at least q% of the strings. We introduce qPMS9, a parallel exact qPMS algorithm that offers significant runtime improvements on DNA and protein datasets. qPMS9 solves the challenging DNA (ℓ, d)-instances (28, 12) and (30, 13). The source code is available at https://code.google.com/p/qpms9/.
Within-visit blood pressure variability is associated with prediabetes and diabetes
Authors: Okada Rieko , Yasuda Yoshinari , Tsushita Kazuyo , Wakai Kenji , Hamajima Nobuyuki , Matsuo Seiichi 
Published: January 2015

AbstractWe investigated the associations between within-visit blood pressure variability (BPV) and risk factors for cardiovascular disease (CVD). The study subjects included 17,795 people aged 40–74 years who underwent health check-ups in Aichi Prefecture, Japan and completed two blood pressure measurements. Subjects were categorized into three groups according to the difference of systolic blood pressure (ΔSBP), namely, low-BPV (≤10 mmHg), moderate-BPV (11–20 mmHg) and high-BPV (>20 mmHg). Subjects were also divided into three categories as those without prediabetes (glycosylated hemoglobin A1c [HbA1c] < 5.7%), prediabetes (HbA1c 5.7–6.4%) and diabetes (HbA1c ≥ 6.5% or under treatment for diabetes). The proportion of prediabetes and diabetes were significantly higher in subjects with high-BPV than in those with low-BPV after adjusting for age, sex and mean SBP (odds ratio [95% confidence interval] was 1.16 [1.01–1.33] for prediabetes and 1.33 [1.06–1.66] for diabetes). Other CVD risk factors were not associated with high-BPV after the adjustment. In conclusion, increased within-visit BPV was significantly associated with the prevalence of prediabetes and diabetes, independent of mean SBP, in a large general population. Therefore, assessing BPV in a single visit may help to identify subjects at increased risk of impaired glycemic control.