bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decorative art of Russian painted spinning wheels and the art of painted lacquer products in China during the Warring States of Qi Lien
Authors: du juan 
Published: June 2024

The article is devoted to the comparison of Mezen painted spinning wheels actively produced in Arkhangelsk province (Russia) in the XIX century and objects of Chinese art of Qi Lien painting of the Warring Kingdoms period. In this era, the skill of creating Chinese lacquer products was very developed, and the spinning wheel industry of the XIX century became the basis for the existence of a vital craft for Russian people. The spinning wheels were decorated with colored paintings with repetitive plots. The subjects of the study represent the most important part of the folk art of the two cultures, reflecting at the same time the aesthetic and spiritual preferences of the population. Their prevalence in both cases made it possible to identify the commonality of artistic techniques used by folk craftsmen. Scientific work assigns an important role to the techniques of manufacturing household items that demonstrate the prevailing artistic environment at the time of their regular use. The leading method of research is formal stylistic analysis, revealing characteristic compositional features. Comparative analysis makes it possible to identify similarities and differences in the works of DPI, which make it possible to hypothesize a paradoxical objective community. Considerable attention is also paid to the coloristic analysis and symbolic meaning of the murals. The scientific novelty of the study is due to the presence of a very limited number of sources mentioning the possible similarity of the two fields of folk art, none of which is fully devoted to the formal and stylistic features of objects created by unknown masters. The article closes this gap, aiming to discover specific unifying features. Its tasks become a detailed study of plots, compositions, techniques and materials used. The research allows us to come to the conclusion that the work of both Russian and Chinese artists reflected the main artistic aspirations of contemporaries, who often endowed objects and phenomena with symbolic meaning. There is a noticeable interest in the use of wood, the use of warm shades and the embedding of patterns in compositions. There is a similarity in the desire to reflect reality when including plant and animal motifs in their works. Also, the decorative and planar nature of the painting combines the rich details of the product. The relevance of the research is due to the convergence of Russian and Chinese cultures, so that the study of the issue can contribute to finding new points of spiritual contact.
Functional variation among mesenchymal stem cells derived from different tissue sources
Authors: Yi Ning , Zeng Qiao , Zheng Chunbing , Li Shiping , Lv Bo , Wang Cheng , Li Chanyi , Jiang Wenjiao , Liu Yun , Yang Yuan , Yan Tenglong , Xue Jinfeng , Xue Zhigang 
Published: June 2024

Background
Mesenchymal stem cells (MSCs) are increasingly recognized for their regenerative potential. However, their clinical application is hindered by their inherent variability, which is influenced by various factors, such as the tissue source, culture conditions, and passage number.

Methods
MSCs were sourced from clinically relevant tissues, including adipose tissue-derived MSCs (ADMSCs, n = 2), chorionic villi-derived MSCs (CMMSCs, n = 2), amniotic membrane-derived MSCs (AMMSCs, n = 3), and umbilical cord-derived MSCs (UCMSCs, n = 3). Passages included the umbilical cord at P0 (UCMSCP0, n = 2), P3 (UCMSCP3, n = 2), and P5 (UCMSCP5, n = 2) as well as the umbilical cord at P5 cultured under low-oxygen conditions (UCMSCP5L, n = 2).

Results
We observed that MSCs from different tissue origins clustered into six distinct functional subpopulations, each with varying proportions. Notably, ADMSCs exhibited a higher proportion of subpopulations associated with vascular regeneration, suggesting that they are beneficial for applications in vascular regeneration. Additionally, CMMSCs had a high proportion of subpopulations associated with reproductive processes. UCMSCP5 and UCMSCP5L had higher proportions of subpopulations related to female reproductive function than those for earlier passages. Furthermore, UCMSCP5L, cultured under low-oxygen (hypoxic) conditions, had a high proportion of subpopulations associated with pro-angiogenic characteristics, with implications for optimizing vascular regeneration.

Conclusions
This study revealed variation in the distribution of MSC subpopulations among different tissue sources, passages, and culture conditions, including differences in functions related to vascular and reproductive system regeneration. These findings hold promise for personalized regenerative medicine and may lead to more effective clinical treatments across a spectrum of medical conditions.
ULK2 suppresses ovarian cancer cell migration and invasion by elevating IGFBP3
Authors: Chen Xiaoxi , Shao Changxiang , Liu Jing , Sun Huizhen , Yao Bingyi , Ma Chengbin , Xu Han , Zhu Weipei 
Published: June 2024

Background
Ovarian cancer is an aggressive malignancy with high mortality known for its considerable metastatic potential. This study aimed to explore the expression and functional role of Unc-51 like autophagy activating kinase 2 (ULK2) in the progression of ovarian cancer.

Methods
ULK2 expression patterns in ovarian cancer tissues as well as benign tumor control samples obtained from our institution were evaluated using immunohistochemistry. Cell counting kit 8 and Transwell assays were applied to assess the effects of ULK2 overexpression on cell proliferation, migration and invasion, respectively. RNA sequencing was performed to explore potential mechanisms of action of ULK2 beyond its classical autophagy modulation.

Results
Our experiments showed significant downregulation of ULK2 in ovarian cancer tissues. Importantly, low expression of ULK2 was markedly correlated with decreased overall survival. In vitro functional studies further demonstrated that overexpression of ULK2 significantly suppressed tumor cell proliferation, migration, and invasion. RNA sequencing analysis revealed a potential regulatory role of ULK2 in the insulin signaling pathway through upregulation of insulin-like growth factor binding protein-3 (IGFBP3) in ovarian cancer cells.

Conclusions
In summary, the collective data indicated that ULK2 acted as a tumor suppressor in ovarian cancer by upregulating the expression of IGFBP3. Our study underscores the potential utility of ULK2 as a valuable prognostic marker for ovarian cancer.
Food addiction and the physical and mental health status of adults with overweight and obesity
Authors: Zielińska Magdalena , Łuszczki Edyta , Szymańska Anna , Dereń Katarzyna 
Published: June 2024

Background
Overweight and obesity now affect more than a third of the world’s population. They are strongly associated with somatic diseases, in particular increasing the risk of many metabolic and cardiovascular diseases, but also with mental disorders. In particular, there is a strong association between obesity and depression. As a result, more attention is paid to the neurobiological, behavioural, and psychological mechanisms involved in eating. One of these is food addiction (FA). Research comparing lifestyle elements, physical and mental health problems of excess body weight and individuals with FA is limited and has focused on younger people, mainly students. There is also a lack of studies that relate actual metabolic parameters to FA. To better understand the problem of FA also in older adults, it is important to understand the specific relationships between these variables.

Methods
A cross-sectional survey was conducted with 172 adults with overweight and obesity (82% female) aged 23–85 years. The mean age of all subjects was M = 59.97 years (SD = 11.93), the mean BMI was M = 32.05 kg/m2 (SD = 4.84), and the mean body fat was M = 39.12% (SD = 6.48). The following questionnaires were used: Food Frequency Questionnaire-6 (FFQ-6), Global Physical Activity Questionnaire (GPAQ), Three Factor Eating Questionnaire-R18 (TFEQ-R18), Yale Food Addiction Scale 2. 0 (YFAS 2.0), Zung Self-Rating Depression Scale (SDS). Body composition, anthropometry, fasting glucose, lipid profile, and blood pressure were measured.

Results
A total of 22.7% of participants with overweight and obesity had symptoms of depression according to the SDS, and 18.6% met the criteria for FA according to YFAS 2.0. FA was statistically significantly more common among people up to 50 years. BMI, body fat mass, diastolic blood pressure and sedentary behaviour were statistically significantly higher in people with FA symptoms. Those who were sedentary for 301–450 min per day were significantly more likely to have depressive symptoms, and those who were sedentary for more than 450 min per day were significantly more likely to have FA symptoms.

Conclusions
Our findings complement the current literature on FA, particularly in older adults and metabolic parameters, and suggest further research directions. Although our cross-sectional study design does not allow causal interpretations, increasing physical activity appears to be particularly important in the management of people with overweight or obesity and FA. This may be even more important than for people with depression alone, but future research is needed to explore these relationships further.
A review of predator exclusion fencing to create mainland islands in Hawaiʻi
Authors: Young Lindsay , VanderWerf Eric 
Published: June 2024

Background
Invasive species are the primary threat to island ecosystems globally and are responsible for approximately two-thirds of all island species extinctions in the past 400 years. Non-native mammals—primarily rats, cats, mongooses, goats, sheep, and pigs—have had devastating impacts on at-risk species and are major factors in population declines and extinctions in Hawaiʻi. With the development of fencing technology that can exclude all mammalian predators, the focus for some locations in Hawaiʻi shifted from predator control to local eradication.

Methods
This article describes all existing and planned full predator exclusion fences in Hawaiʻi by documenting the size and design of each fence, the outcomes the predator eradications, maintenance issues at each fence, and the resulting native species responses.

Results
Twelve predator exclusion fences were constructed in the Hawaiian Islands from 2011–2023 and six more were planned or under construction; all were for the protection of native seabirds and waterbirds. Fences ranged in length from 304–4,877 m and enclosed 1.2–640 ha. One-third of the 18 fences were peninsula-style with open ends; the remaining two-thirds of the fences were complete enclosures. The purpose of twelve of the fences (67%) was to protect existing bird populations, and six (33%) were initiated for mitigation required under the U.S. Endangered Species Act. Of the six mitigation fences, 83% were for the social attraction of seabirds and one fence was for translocation of seabirds; none of the mitigation fences protected existing bird populations. Rats and mice were present in every predator exclusion fence site; mice were eradicated from five of six sites (83%) where they were targeted and rats (three species) were eradicated from eight of 11 sites (72%). Mongoose, cats, pigs, and deer were eradicated from every site where they were targeted. Predator incursions occurred in every fence. Rat and mouse incursions were in many cases chronic or complete reinvasions, but cat and mongoose incursions were occasional and depended on fence type (i.e., enclosed vs. peninsula). The advent of predator exclusion fencing has resulted in great gains for protecting existing seabirds and waterbirds, which demonstrated dramatic increases in reproductive success and colony growth. With threats from invasive species expected to increase in the future, predator exclusion fencing will become an increasingly important tool in protecting island species.
The Relationship between Academic Mindset and Academic Performance: A Case of Gedu College of Business Studies, Bhutan
Authors: Wangmo Sonam , Wangpo Kinga 
Published: June 2024

Aims: This study attempted to assess how students’ academic mindset influences their academic performance and secondly, whether academic mindset differ across demographic variables like gender. Four academic mindset factors were examined by the researchers.
Study Design: A quantitative research design with a descriptive study was used in this study.
Place and Duration of Study: The study was conducted in Bhutan for a period of one year.
Methodology: A total of 314 students from Gedu College of Business studies of Bhutan participated in the study using an online structured questionnaire developed by the researchers and shared through Google Forms. Simple random sampling technique was employed to draw the samples from the given population. The results were analyzed and presented using regression analysis and independent T-Test.
Results: According to the findings, there is a positive relationship between variables of academic mindset such as self-efficacy and growth mindset with academic performance. Secondly, in terms of gender, there is difference in terms of view on the self-efficacy variable.
Conclusion: The key recommendations of the study is for intervention from academic institutions to help students develop a growth mindset and also improve the self-efficacy of the students to draw better academic results.
The NCTC 2544 IL‐18 Assay for the In Vitro Identification of Contact Allergens
Authors: Galbiati Valentina , Corsini Emanuela 
Published: November 2012

AbstractAssessment of allergenic potency of low‐molecular‐weight compounds is generally performed using animal models, such as the murine Local Lymph Node Assay (LLNA) and the Guinea Pig Maximization Test (GMT). Progress in understanding the mechanism of skin sensitization, including effects on the production of cytokines by different cell types within the skin, provides the opportunity to develop in vitro tests as an alternative to in vivo sensitization testing. This unit will describe a method for differentiating contact allergens from low‐molecular‐weight respiratory allergens and irritants, based on the selective induction of intracellular interleukin 18 (IL‐18) in the human keratinocyte cell line NCTC 2544. Similar results could also be obtained using primary human keratinocytes or other human keratinocyte cell lines, e.g., HaCaT, HPKII, etc. IL‐18 was chosen because this cytokine has been demonstrated to favor Th‐1‐type immune responses by enhancing the secretion of pro‐inflammatory mediators such as TNF‐α, IL‐8, and IFN‐γ, and to play a key proximal role in the induction of allergic contact dermatitis. Curr. Protoc. Toxicol. 54:20.8.1‐20.8.18. © 2012 by John Wiley & Sons, Inc.
Membrane Vesicle ABC Transporter Assays for Drug Safety Assessment
Authors: van Staden Carlo J. , Morgan Ryan E. , Ramachandran Bharath , Chen Yuan , Lee Paul H. , Hamadeh Hisham K. 
Published: November 2012

AbstractThe use of plasma membrane vesicles that overexpress the bile salt export pump (BSEP) or multidrug resistance−associated protein 2, 3, or 4 (MRP2‐4) with an in vitro vacuum filtration system offers a rapid and reliable means for screening drug candidates for their effects on transporter function in hepatocytes and thus their potential for causing drug‐induced liver injury (DILI). Comparison of transporter activity in the presence and absence of ATP allows for determination of a specific assay window for each transporter. This window is used to determine the degree to which each test compound inhibits transporter activity. This assay battery is helpful for prioritizing and rank‐ordering compounds within a chemical series with respect to each other and in the context of known inhibitors of transporter activity and/or liver injury. This model can be used to influence the drug development process at an early stage and provide rapid feedback regarding the selection of compounds for advancement to in vivo safety evaluations. A detailed protocol for the high‐throughput assessment of ABC transporter function is provided, including specific recommendations for curve‐fitting to help ensure consistent results. Curr. Protoc. Toxicol. 54:23.5.1‐23.5.24. © 2012 by John Wiley & Sons, Inc.
Association of herbal cannabis use with negative psychosocial parameters in patients with fibromyalgia
Authors: Ste‐Marie Peter A. , Fitzcharles Mary‐Ann , Gamsa Ann , Ware Mark A. , Shir Yoram 
Published: November 2012

AbstractObjectivePatients with chronic pain, including fibromyalgia (FM), may seek treatments outside of mainstream medicine. Medicinal cannabinoids are popularly advocated for pain relief but with limited evidence for efficacy in FM. The extent of use of cannabinoids in FM is unknown.MethodsWe have documented the self‐reported prevalence of cannabinoid use in 457 patients with the diagnosis of FM and referred to a tertiary care pain center. We validated the diagnosis of FM and examined the associations of cannabinoid use in these patients.ResultsCannabinoids were being used by 13% of all patients, of whom 80% used herbal cannabis (marijuana), 24% used prescription cannabinoids, and 3% used both herbal cannabis and prescription cannabinoids. One‐third of all men used cannabinoids. Current unstable mental illness (36% versus 23%; P = 0.002), opioid drug–seeking behavior (17% versus 4%; P = 0.002), and male sex (26% versus 7%; P = 0.0002) were all associated with herbal cannabis use. There was a trend for cannabinoid users to be unemployed and receiving disability payments. The diagnosis of FM was validated in 302 patients, with 155 assigned another primary diagnosis. When the FM group was analyzed separately, significant associations were lost, but trends remained.ConclusionCannabinoids were used by 13% of patients referred with a diagnosis of FM. The association of herbal cannabis use with negative psychosocial parameters raises questions regarding the motive for this self‐medication practice. Although cannabinoids may offer some therapeutic effect, caution regarding any recommendation should be exercised pending clarification of general health and psychosocial problems, especially for those self‐medicating.
Taxonomy for systemic lupus erythematosus with onset before adulthood
Authors: Silva Clovis A. , Avcin Tadej , Brunner Hermine I. 
Published: November 2012

AbstractObjectiveTo propose a common nomenclature to refer to individuals who fulfill the American College of Rheumatology classification criteria for systemic lupus erythematosus (SLE) during childhood or adolescence.MethodsThe medical literature was reviewed for studies conducted in the target population between 1960 and December 2011 to obtain information about the terms used to refer to such children and adolescents. We reviewed the threshold ages used and disease features considered to discriminate these individuals from patients with onset of SLE during adulthood. Furthermore, the nomenclature used in other chronic diseases with onset during both childhood and adulthood was assessed.ResultsThere was an astonishing variability in the age cutoffs used to define SLE onset prior to adulthood, ranging from 14–21 years, but most studies used age 18 years. The principal synonyms in the medical literature were SLE without reference to the age at onset of disease, childhood‐onset SLE, juvenile SLE, and pediatric (or paediatric) SLE.ConclusionBased on the definition of childhood, in analogy with other complex chronic diseases commencing prior to adulthood, and given the current absence of definite genetic variations that discriminate adults from children, the term childhood‐onset SLE is proposed when referring to individuals with onset of SLE prior to age 18 years.
Obesity and reduction of the response rate to anti–tumor necrosis factor α in rheumatoid arthritis: An approach to a personalized medicine
Authors: Gremese Elisa , Carletto Antonio , Padovan Melissa , Atzeni Fabiola , Raffeiner Bernd , Giardina Anna Rita , Favalli Ennio Giulio , Erre Gian Luca , Gorla Roberto , Galeazzi Mauro , Foti Rosario , Cantini Fabrizio , Salvarani Carlo , Olivieri Ignazio , Lapadula Giovanni , Ferraccioli Gianfranco ,   for the Gruppo Italiano di Studio sulle Early Arthritis (GISEA)
Published: November 2012

AbstractObjectiveObesity is a mild, long‐lasting inflammatory disease and, as such, could increase the inflammatory burden of rheumatoid arthritis (RA). The study aim was to determine whether obesity represents a risk factor for a poor remission rate in RA patients requiring anti–tumor necrosis factor α (anti‐TNFα) therapy for progressive and active disease despite treatment with methotrexate or other disease‐modifying antirheumatic drugs.MethodsPatients were identified from 15 outpatient clinics of university hospitals and hospitals in Italy taking part in the Gruppo Italiano di Studio sulle Early Arthritis network. Disease Activity Score in 28 joints (DAS28), body mass index (BMI; categorized as <25, 25–30, and >30 kg/m2), acute‐phase reactants, IgM rheumatoid factor, and anti–cyclic citrullinated peptide antibody values were collected. DAS28 remission was defined as a score of <2.6 lasting for at least 3 months.ResultsSix hundred forty‐one outpatients with longstanding RA receiving anti‐TNFα blockers (adalimumab, n = 260; etanercept, n = 227; infliximab, n = 154), recruited from 2006–2009 and monitored for at least 12 months, were analyzed. The mean ± SD DAS28 at baseline was 5.6 ± 1.4. A BMI of >30 kg/m2 was recorded in 66 (10.3%) of 641 RA patients. After 12 months of anti‐TNFα treatment, a DAS28 of <2.6 was noted in 15.2% of the obese subjects, in 30.4% of the patients with a BMI of 25–30 kg/m2, and in 32.9% of the patients with a BMI of <25 kg/m2 (P = 0.01). The lowest percentage of remission, which was statistically significant versus adalimumab and etanercept (P = 0.003), was observed with infliximab.ConclusionObesity represents a risk factor for a poor remission rate in patients with longstanding RA treated with anti‐TNFα agents. A personalized treatment plan might be a possible solution.
Comparison of three methods for calculating the Bath Ankylosing Spondylitis Metrology Index in a randomized placebo‐controlled study
Authors: van der Heijde Désirée , Deodhar Atul , Inman Robert D. , Braun Jürgen , Hsu Ben , Mack Michael 
Published: November 2012

AbstractObjectiveTo compare 3 methods for calculating the Bath Ankylosing Spondylitis Metrology Index (BASMI) score using patients who participated in the GO‐RAISE study.MethodsPatients with active ankylosing spondylitis (AS) were randomly assigned in a 1:1.8:1.8 ratio to receive subcutaneous injections of placebo, golimumab 50 mg, or golimumab 100 mg every 4 weeks. Lumbar flexion, tragus‐to‐wall distance, lumbar side flexion, intermalleolar distance, and cervical rotation angle measurements at baseline, week 14, and week 24 were used to calculate the BASMI 2‐step (BASMI2), 10‐step (BASMI10), and linear (BASMIlin) scores.ResultsBASMI2 scores were generally lower than BASMI10 and BASMIlin scores, which were nearly identical. Median changes from baseline to week 14 in the combined golimumab group were similar to those in the placebo group when using the BASMI2 calculation method (0.00 versus 0.00; P = 0.288). The combined golimumab group showed significantly greater improvement from baseline to week 14 than the placebo group when using the BASMI10 (−0.20 versus 0.00; P = 0.018) and BASMIlin (−0.31 versus −0.07; P = 0.015) calculation methods, with the latter showing the greatest difference between golimumab and placebo. Guyatt's effect size was better for the BASMIlin and the BASMI10 versus the BASMI2 in the combined golimumab group at week 14 (0.58 and 0.53 versus 0.42, respectively) and week 24 (0.76 and 0.69 versus 0.61, respectively), despite the relatively short period to assess changes in spinal mobility.ConclusionThe BASMIlin method was the most sensitive to changes in range of motion exhibited by patients with AS who received golimumab.