bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell surface engineering using glycosylphosphatidylinositol anchored tissue inhibitor of matrix metalloproteinase‐1 stimulates cutaneous wound healing
Authors: Djafarzadeh Roghieh , Conrad Claudius , Notohamiprodjo Susan , Hipp Stephanie , Niess Hanno , Bruns Christiane J. , Nelson Peter J. 
Published: January 2014

AbstractThe balance between matrix metalloproteinases and their endogenous tissue inhibitors (TIMPs) is an important component in effective wound healing. The biologic action of these proteins is linked in part to the stoichiometry of TIMP/matrix metalloproteinases/surface protein interactions. We recently described the effect of a glycosylphosphatidylinositol (GPI) anchored version of TIMP‐1 on dermal fibroblast biology. Here, cell proliferation assays, in vitro wound healing, electrical wound, and impedance measurements were used to characterize effects of TIMP‐1‐GPI treatment on primary human epidermal keratinocytes. TIMP‐1‐GPI stimulated keratinocyte proliferation, as well as mobilization and migration. In parallel, it suppressed the migration and matrix secretion of dermal myofibroblasts, and reduced their secretion of active TGF‐β1. Topical application of TIMP‐1‐GPI in an in vivo excisional wound model increased the rate of wound healing. The agent positively influenced different aspects of wound healing depending on the cell type studied. TIMP‐1‐GPI counters potential negative effects of overactive myofibroblasts and enhances the mobilization and proliferation of keratinocytes essential for effective wound healing. The application of TIMP‐1‐GPI represents a novel and practical clinical solution for facilitating healing of difficult wounds.
Laboratory Identification of Arthropod Ectoparasites
Authors: Mathison Blaine A. , Pritt Bobbi S. 
Published: January 2014

SUMMARYThe collection, handling, identification, and reporting of ectoparasitic arthropods in clinical and reference diagnostic laboratories are discussed in this review. Included are data on ticks, mites, lice, fleas, myiasis-causing flies, and bed bugs. The public health importance of these organisms is briefly discussed. The focus is on the morphological identification and proper handling and reporting of cases involving arthropod ectoparasites, particularly those encountered in the United States. Other arthropods and other organisms not of public health concern, but routinely submitted to laboratories for identification, are also briefly discussed.
Early-Onset Neonatal Sepsis
Authors: Simonsen Kari A. , Anderson-Berry Ann L. , Delair Shirley F. , Davies H. Dele 
Published: January 2014

SUMMARYEarly-onset sepsis remains a common and serious problem for neonates, especially preterm infants. Group B streptococcus (GBS) is the most common etiologic agent, whileEscherichia coliis the most common cause of mortality. Current efforts toward maternal intrapartum antimicrobial prophylaxis have significantly reduced the rates of GBS disease but have been associated with increased rates of Gram-negative infections, especially among very-low-birth-weight infants. The diagnosis of neonatal sepsis is based on a combination of clinical presentation; the use of nonspecific markers, including C-reactive protein and procalcitonin (where available); blood cultures; and the use of molecular methods, including PCR. Cytokines, including interleukin 6 (IL-6), interleukin 8 (IL-8), gamma interferon (IFN-γ), and tumor necrosis factor alpha (TNF-α), and cell surface antigens, including soluble intercellular adhesion molecule (sICAM) and CD64, are also being increasingly examined for use as nonspecific screening measures for neonatal sepsis. Viruses, in particular enteroviruses, parechoviruses, and herpes simplex virus (HSV), should be considered in the differential diagnosis. Empirical treatment should be based on local patterns of antimicrobial resistance but typically consists of the use of ampicillin and gentamicin, or ampicillin and cefotaxime if meningitis is suspected, until the etiologic agent has been identified. Current research is focused primarily on development of vaccines against GBS.
Gamma Interferon Release Assays for Detection of Mycobacterium tuberculosis Infection
Authors: Pai Madhukar , Denkinger Claudia M. , Kik Sandra V. , Rangaka Molebogeng X. , Zwerling Alice , Oxlade Olivia , Metcalfe John Z. , Cattamanchi Adithya , Dowdy David W. , Dheda Keertan , Banaei Niaz 
Published: January 2014

SUMMARY

Identification and treatment of latent tuberculosis infection (LTBI) can substantially reduce the risk of developing active disease. However, there is no diagnostic gold standard for LTBI. Two tests are available for identification of LTBI: the tuberculin skin test (TST) and the gamma interferon (IFN-γ) release assay (IGRA). Evidence suggests that both TST and IGRA are acceptable but imperfect tests. They represent indirect markers of
Mycobacterium tuberculosis
exposure and indicate a cellular immune response to
M. tuberculosis
. Neither test can accurately differentiate between LTBI and active TB, distinguish reactivation from reinfection, or resolve the various stages within the spectrum of
M. tuberculosis
infection. Both TST and IGRA have reduced sensitivity in immunocompromised patients and have low predictive value for progression to active TB. To maximize the positive predictive value of existing tests, LTBI screening should be reserved for those who are at sufficiently high risk of progressing to disease. Such high-risk individuals may be identifiable by using multivariable risk prediction models that incorporate test results with risk factors and using serial testing to resolve underlying phenotypes. In the longer term, basic research is necessary to identify highly predictive biomarkers.
Acute Recovery of Oral Word Production Following Stroke: Patterns of Performance as Predictors of Recovery
Authors: Cloutman Lauren , Newhart Melissa , Davis Cameron , Heidler-Gary Jennifer , Hillis Argye E. 
Published: January 2014

Background:Impairments in oral word production are common at the onset of stroke. The identification of factors that predict early recovery has important implications for identifying those at greater risk of continued impaired functioning, and the management of the patient's care following discharge.Aims:To identify patterns of performance that are predictors of acute recovery of oral word production abilities following stroke; to identify any association between early and more chronic recovery.Method and procedures:Acute stroke patients were administered oral word production tasks within 1–2 days of hospital admission, with repeat testing by 7 days; a subset of patients had repeat testing between three weeks to one year later. Performance was examined for error rate and type to identify potential predictors of early recovery.Outcome and results:The proportion of circumlocution and no response errors at initial testing were associated with the magnitude of recovery of language functioning within the first week following stroke. Patient characteristics of age and gender were found to have no influence on the degree of early recovery observed. None of the examined factors predicted late recovery. The degree of early recovery was not associated with the degree of later recovery.Conclusions:The current study identified patterns of task performance that increase our understanding of how oral word production recovers following acute stroke. The finding that the degree of early recovery does not predict the degree of later recovery is consistent with the hypothesis that early and late recovery are due to different mechanisms (restored blood flow in acute stroke, and reorganization in later recovery).
Automatic Bias of Temporal Expectations following Temporally Regular Input Independently of High-level Temporal Expectation
Authors: Breska Assaf , Deouell Leon Y. 
Published: January 2014

Abstract
Exposure to rhythmic stimulation results in facilitated responses to events that appear in-phase with the rhythm and modulation of anticipatory and target-evoked brain activity, presumably reflecting “exogenous,” unintentional temporal expectations. However, the extent to which this effect is independent from intentional processes is not clear. In two EEG experiments, we isolated the unintentional component of this effect from high-level, intentional factors. Visual targets were presented either in-phase or out-of-phase with regularly flickering colored stimuli. In different blocks, the rhythm could be predictive (i.e., high probability for in-phase target) or not, and the color could be predictive (i.e., validly cue the interval to the target) or not. Exposure to nonpredictive rhythms resulted in faster responses for in-phase targets, even when the color predicted specific out-of-phase target times. Also, the contingent negative variation, an EEG component reflecting temporal anticipation, followed the interval of the nonpredictive rhythm and not that of the predictive color. Thus, rhythmic stimulation unintentionally induced expectations, even when this was detrimental. Intentional usage of predictive rhythms to form expectations resulted in a stronger behavioral effect, and only predictive cues modulated the latency of the target-evoked P3, presumably reflecting stimulus evaluation. These findings establish the existence of unintentional temporal expectations in rhythmic contexts, dissociate them from intentional expectations, and highlight the need to distinguish between the source of expectation (exogenous–endogenous) and the level of voluntary control involved in it (unintentional–intentional).
Les stratégies de neutralisation de la pression normative par les consommateurs : le cas du téléchargement illégal
Authors: Odou Philippe , Bonnin Gaël 
Published: January 2014

Résumé Les techniques de neutralisation, qui permettent aux consommateurs d’accomplir des pratiques déviantes tout en restant attachés à la norme de référence, sont mal connues. Nous montrons, dans le cas du téléchargement illégal, qu’il s’agit d’un assemblage discursif autonome, articulé autour de trois stratégies défensives (déresponsabilisation neutralisatrice, neutralisation pragmatique, neutralisation idéologique), et supportant la contradiction interne.
Using Benchmarking Techniques and the 2011 Maternity Practices Infant Nutrition and Care (mPINC) Survey to Improve Performance among Peer Groups across the United States
Authors: Edwards Roger A. , Dee Deborah , Umer Amna , Perrine Cria G. , Shealy Katherine R. , Grummer-Strawn Laurence M. 
Published: January 2014

Background: A substantial proportion of US maternity care facilities engage in practices that are not evidence-based and that interfere with breastfeeding. The CDC Survey of Maternity Practices in Infant Nutrition and Care (mPINC) showed significant variation in maternity practices among US states. Objective: The purpose of this article is to use benchmarking techniques to identify states within relevant peer groups that were top performers on mPINC survey indicators related to breastfeeding support. Methods: We used 11 indicators of breastfeeding-related maternity care from the 2011 mPINC survey and benchmarking techniques to organize and compare hospital-based maternity practices across the 50 states and Washington, DC. We created peer categories for benchmarking first by region (grouping states by West, Midwest, South, and Northeast) and then by size (grouping states by the number of maternity facilities and dividing each region into approximately equal halves based on the number of facilities). Results: Thirty-four states had scores high enough to serve as benchmarks, and 32 states had scores low enough to reflect the lowest score gap from the benchmark on at least 1 indicator. No state served as the benchmark on more than 5 indicators and no state was furthest from the benchmark on more than 7 indicators. The small peer group benchmarks in the South, West, and Midwest were better than the large peer group benchmarks on 91%, 82%, and 36% of the indicators, respectively. In the West large, the Midwest large, the Midwest small, and the South large peer groups, 4-6 benchmarks showed that less than 50% of hospitals have ideal practice in all states. Conclusion: The evaluation presents benchmarks for peer group state comparisons that provide potential and feasible targets for improvement.
Characterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China
Authors: Meng Qiong , Bai Xiangning , Zhao Ailan , Lan Ruiting , Du Huamao , Wang Tao , Shi Changyou , Yuan Xuejiao , Bai Xuemei , Ji Shaobo , Jin Dong , Yu Bo , Wang Yan , Sun Hui , Liu Kai , Xu Jianguo , Xiong Yanwen 
Published: January 2014

AbstractBackgroundShiga toxin-producingEscherichia coli(STEC) is recognized as an important human diarrheal pathogen. Swine plays an important role as a carrier of this pathogen. In this study we determined the prevalence and characteristics of STEC from healthy swine collected between May 2011 and August 2012 from 3 cities/provinces in China.ResultsA total of 1003 samples, including 326 fecal, 351 small intestinal contents and 326 colon contents samples, was analyzed. Two hundred and fifty five samples werestx-positive by PCR and 93 STEC isolates were recovered from 62stx-positive samples. Twelve O serogroups and 19 O:H serotypes including 6 serotypes (O100:H20/[H20], O143:H38/[H38], O87:H10, O172:H30/[H30], O159:H16, O9:H30/[H30]) rarely found in swine and ruminants were identified. All 93 STEC isolates harboredstx2only, all of which werestx2esubtype including 1 isolate being a new variant ofstx2e. 53.76%, 15.05% and 2.15% STEC isolates carriedastA,hlyAandehxArespectively. Four STEC isolates harbored the high-pathogenicity island. Of the 15 adherence-associated genes tested, 13 (eae,efa1,iha,lpfAO113,lpfAO157/OI-154,lpfAO157/OI-141,toxB,saa, F4, F5, F6, F17 or F41) were all absent while 2 (paaand F18) were present in 7 and 4 STEC isolates respectively. The majority of the isolates were resistant to tetracycline (79.57%), nalidixic acid (78.49%), trimethoprim-sulfamethoxazole (73.12%) and kanamycin (55.91%). The STEC isolates were divided into 63 pulsed-field gel electrophoresis patterns and 21 sequence types (STs). Isolates of the same STs generally showed the same or similar drug resistance patterns. A higher proportion of STEC isolates from Chongqing showed multidrug resistance with one ST (ST3628) resistant to 14 antimicrobials.ConclusionsOur results indicate that swine is a significant reservoir of STEC strains in China. Based on comparison by serotypes and sequence types with human strains and presence of virulence genes, the swine STEC may have a low potential to cause human disease.
Sulfotransferase 1A1 (SULT1A1) gene expression is regulated by members of the NFI transcription factors in human breast cancer cells
Authors: Yao-Borengasser Aiwei , Rogers Lora J , Edavana Vineetha K , Penney Rosalind B , Yu Xinfeng , Dhakal Ishwori B , Williams Suzanne , Kadlubar Susan A 
Published: January 2014

Abstract

Background
Sulfotransferase 1A1 (SULT1A1) gene expression is tissue specific, with little to no expression in normal breast epithelia. Expression in breast tumors has been documented, but the transcriptional regulation of SULT1A1 in human breast tissue is poorly understood. We identified Nuclear Factor I (NFI) as a transcription factor family involved in the regulation of SULT1A1 expression.

Methods
Transcription Factor Activation Profiling Plate Array assay was used to identify the possible transcription factors that regulate the gene expression of SULT1A1 in normal breast MCF-10A cells and breast cancer ZR-75-1 cells. Expression levels of NFI-C and SULT1A1 were determined by real-time RT-PCR using total RNA isolated from 84 human liver samples. Expression levels of SULT1A1, NFI-A, NFI-B, NFI-C, and NFI-X were also determined in different human breast cancer cell lines (MCF-7, T-47D, ZR-75-1, and MDA-MB-231), in the transformed human epithelial cell line MCF-10A, and in ZR-75-1 cells that were transfected with siRNAs directed against NFI-A, NFI-B, NFI-C, or NFI-X for 48 h. The copy numbers of SULT1A1 in cell lines ZR-75-1, MCF-7, T-47D, MDA-MB-231, and MCF-10A were determined using a pre-designed Custom Plus TaqMan® Copy Number kit from Life Technologies.

Results
In normal human liver samples, SULT1A1 mRNA level was positively associated with NFI-C. In different human breast cancer and normal epithelial cell lines, SULT1A1 expression was positively correlated with NFI-B and NFI-C. SULT1A1 expression was decreased 41% and 61% in ZR-75-1 cells treated with siRNAs against NFI-A and NFI-C respectively. SULT1A1 gene expression was higher in cells containing more than one SULT1A1 copy numbers.

Conclusions
Our data suggests that SULT1A1 expression is regulated by NFI, as well as SULT1A1 copy number variation in human breast cancer cell lines. These data provide a mechanistic basis for the differential expression of SULT1A1 in different tissues and different physiological states of disease.
The antiproliferative effect of C2-ceramide on lung cancer cells through apoptosis by inhibiting Akt and NFκB
Authors: Lin I-Ling , Chou Han-Lin , Lee Jin-Ching , Chen Feng-Wei , Fong Yao , Chang Wei-Chiao , Huang Hurng Wern , Wu Chang-Yi , Chang Wen-Tsan , Wang Hui-Min David , Chiu Chien-Chih 
Published: January 2014

Abstract
The anticancer effects of ceramide have been reported in many types of cancers but less in lung cancer. In this study, we used C2-ceramide to further investigate its possible anticancer effects and mechanisms on non-small cell lung cancer (NSCLC) H1299 cells. The result of cell proliferation in terms of trypan blue assay showed high dose of C2-ceramide inhibited cell survival after 24 h treatment. The flow cytometry-based assays indicated the effect of apoptosis, chromatin condensation, and G1 arrest in terms of Annexin V/propidium iodide (PI), DAPI, and PI stainings, respectively. Moreover, the decreased protein level of p-Akt, p-NFκB, survivin and cyclin A2 were detected by Western blot assay. Taken together, these results indicated the antiproliferative effect of C2-ceramide is majorly responsible for cell apoptosis in lung cancer H1299 cells.
The InN epitaxy via controlling In bilayer
Authors: Zhou Jin , Huang Qiangcan , Li Jinchai , Cai Duanjun , Kang Junyong 
Published: January 2014

AbstractThe method of In bilayer pre-deposition and penetrated nitridation had been proposed, which had been proven to have many advantages theoretically. To study the growth behavior of this method experimentally, various pulse times of trimethylindium supply were used to get the optimal indium bilayer controlling by metalorganic vapour phase epitaxy. The results revealed that the InN film quality became better as the thickness of the top indium atomic layers was close to bilayer. A following tuning of nitridation process enhanced the quality of InN film further, which means that a moderate, stable, and slow nitridation process by NH3 flow also plays the key role in growing better-quality InN film. Meanwhile, the biaxial strain of InN film was gradually relaxing when the flatness was increasingly improved.
Equivalence of semistability of Picard, Mann, Krasnoselskij and Ishikawa iterations
Authors: Yousefi Bahman , Yadegarnejad Ali , Azadi Kenary Hassan , Park Choonkil 
Published: January 2014

Abstract
In this paper, we show that convergence of Picard, Mann, Krasnoselskij and Ishikawa iterations is equivalent in cone normed spaces. Also, we prove that semistability of these iterations is equivalent.
Fixed point results for modified weak and rational α-ψ-contractions in ordered 2-metric spaces
Authors: Fathollahi Shahin , Hussain Nawab , Khan Liaqat A 
Published: January 2014

Abstract
We first introduce the concept of a triangular 2-α-η-admissible mapping which extends the notion of α-admissible mapping with respect to η to 2-metric spaces. Next, we introduce the concepts of modified weak and modified rational α-ψ-contractions and establish the existence and uniqueness of fixed points for such mappings in complete 2-metric spaces. As an application of the obtained results, we prove some fixed point results in partially ordered 2-metric spaces. The presented theorems generalize and improve certain existing results in the literature and provide main results in Dung and Hang (Fixed Point Theory Appl. 2013:161, 2013) as corollaries. Moreover, some examples and an application to integral equations are provided to illustrate the usability of the obtained results.

MSC:46N40, 47H10, 54H25, 46T99.
"Equation missing" -contractive multivalued mappings
Authors: Ali Muhammad Usman , Kamran Tayyab , Karapınar Erdal 
Published: January 2014

Abstract
In this paper, we introduce the notion of

(
α
,
ψ
,
ξ
)

-contractive multivalued mappings to generalize and extend the notion of α-ψ-contractive mappings to closed valued multifunctions. We investigate the existence of fixed points for such mappings. We also construct an example to show that our result is more general than the results of α-ψ-contractive closed valued multifunctions.

MSC:47H10, 54H25.
Electroacupuncture for pressure ulcer: a study protocol for a randomized controlled pilot trial
Authors: Zhang Qin-hong , Yue Jin-huan , Sun Zhong-ren 
Published: January 2014

Abstract

Background
Pressure ulcers are one of the most common health complaints, which often take months or years to heal, and affect patients’ morbidity and quality of life. Medical options for pressure ulcers are limited. Electroacupuncture (EA) has been employed to relieve the symptoms for patients with pressure ulcers, but there is limited clinical evidence for its effectiveness.

Methods/Design
This study consists of a randomized controlled trial (RCT) with two parallel arms: a control group and an EA group. Both groups will receive standard wound care (including changing position, using mattresses and cushions, and a good diet) of five sessions per week for a total of 40 sessions during the 8-week treatment period. In addition, the EA group will receive the EA intervention. The following outcome measurements will be used in examination of participants: wound surface area (WSA), visual analogue scale (VAS), and the proportion of ulcers healed within trial period (PUHTP). All the outcomes will be evaluated at the start of the study, at the end of the fourth week, at 8 weeks after randomization, and 1 month after treatment cessation.

Discussion
The aim of this study is to evaluate the effectiveness of EA for the treatment of patients with pressure ulcers.

Trial registration
Chinese Clinical Trial Register: ChiCTR-TRC-11001693