bluuebunny/crossref_metadata_2025_split huggingface dataset:
HDAC‐inhibitor (S)‐8 disrupts HDAC6‐PP1 complex prompting A375 melanoma cell growth arrest and apoptosis
Authors: Balliu Manjola , Guandalini Luca , Romanelli Maria Novella , D'Amico Massimo , Paoletti Francesco 
Published: November 2014

AbstractHistone deacetylase inhibitors (HDACi) are agents capable of inducing growth arrest and apoptosis in different tumour cell types. Previously, we reported a series of novel HDACi obtained by hybridizing SAHA or oxamflatin with 1,4‐benzodiazepines. Some of these hybrids proved effective against haematological and solid cancer cells and, above all, compound (S)‐8 has emerged for its activities in various biological systems. Here, we describe the effectiveness of (S)‐8 against highly metastatic human A375 melanoma cells by using normal PIG1 melanocytes as control. (S)‐8 prompted: acetylation of histones H3/H4 and α‐tubulin; G0/G1 and G2/M cell cycle arrest by rising p21 and hypophos‐phorylated RB levels; apoptosis involving the cleavage of PARP and caspase 9, BAD protein augmentation and cytochrome c release; decrease in cell motility, invasiveness and pro‐angiogenic potential as shown by results of wound‐healing assay, down‐regulation of MMP‐2 and VEGF‐A/VEGF‐R2, besides TIMP‐1/TIMP‐2 up‐regulation; and also intracellular accumulation of melanin and neutral lipids. The pan‐caspase inhibitor Z‐VAD‐fmk, but not the antioxidant N‐acetyl‐cysteine, contrasted these events. Mechanistically, (S)‐8 allows the disruption of cytoplasmic HDAC6‐protein phosphatase 1 (PP1) complex in A375 cells thus releasing the active PP1 that dephosphorylates AKT and blocks its downstream pro‐survival signalling. This view is consistent with results obtained by: inhibiting PP1 with Calyculin A; using PPP1R2‐transfected cells with impaired PP1 activity; monitoring drug‐induced HDAC6‐PP1 complex re‐shuffling; and, abrogating HDAC6 expression with specific siRNA. Altogether, (S)‐8 proved very effective against melanoma A375 cells, but not normal melanocytes, and safe to normal mice thus offering attractive clinical prospects for treating this aggressive malignancy.
Pruritus and quality of life in moderate‐to‐severe plaque psoriasis: post hoc explorative analysis from the PRISTINE study
Authors: Mrowietz U. , Chouela E.N. , Mallbris L. , Stefanidis D. , Marino V. , Pedersen R. , Boggs R. L. 
Published: November 2014

AbstractBackgroundPruritus is a clinically important symptom of psoriasis that has a major impact on quality of life (QoL).ObjectiveThe objective of this study was to examine pruritus and QoL in patients with moderate‐to‐severe psoriasis treated with etanercept (ETN) in the PRISTINE clinical trial.MethodsPatients were randomized (1 : 1, double‐blind) to ETN 50 mg QW or 50 mg BIW for 12 weeks, followed by 50 mg QW for 12 weeks. Pruritus was reported as 0 (no itching) to 5 (severe itching). Associations were examined between pruritus and Psoriasis Area and Severity Index, Dermatology Life Quality Index (DLQI), Hospital Anxiety and Depression Screening (HADS), Functional Assessment of Chronic Illness Therapy‐Fatigue (FACIT‐Fatigue), Euro‐Qol 5D (EQ‐5D) and Medical Outcomes Study (MOS) Sleep Index II.ResultsAt baseline, patients (n = 270) had a mean pruritus level of 3.6. Itching (level ≥1) was reported by 96% of patients, 62% of whom had severe itching (level ≥4) and 26% had the highest level of itching. DLQI, HADS‐Anxiety, HADS‐Depression, FACIT‐Fatigue, EQ‐5D visual analog scale, and MOS Sleep Index II were significantly associated with itch. At week 12, mean pruritus improvement in the ETN BIW/QW group was greater than in the QW/QW group (2.4 vs. 1.6, P < 0.001), but not at week 24 (2.2 vs. 2.0, P = 0.180). Patients with the most severe itching at baseline (score of 5) had a mean score of 1.7 at week 24. Overall, patients with clinically meaningful pruritus improvement at week 24 reported greater improvement in QoL measures than other patients.ConclusionMost patients with moderate‐to‐severe psoriasis in this study (96%) reported pruritus. Pruritus improved significantly with ETN therapy and was strongly associated with improvements in QoL. These data support the clinical relevance of pruritus as an important symptom of patients with moderate/severe psoriasis.
Bariatric surgery – a dermatologic perspective
Authors: Wollina U. , Dreßler M. , Lohmann T. 
Published: November 2014

AbstractBariatric surgery has become a standard option in morbid obesity for patients not responding to conventional treatment. A major and stable weight loss can be achieved. Since obesity and weight loss may affect skin diseases, we performed this review to analyse the impact of bariatric surgery on a number of skin diseases. We categorized the skin diseases into three main groups: (i) diseases with a possible benefit from bariatric surgery, (ii) diseases that may develop after bariatric surgery and (iii) diseases that may persist. We hope that dermatologists will achieve an updated knowledge of benefits and possible hazards of this type of surgically induced weight loss.
A novel fish cell line derived from the brain of Chinese perch Siniperca chuatsi: development and characterization
Authors: Fu X. , Li N. , Lai Y. , Luo X. , Wang Y. , Shi C. , Huang Z. , Wu S. , Su J. 
Published: November 2014

In this study, a continuous cell line (named as CPB) was established from Siniperca chuatsi brain and has been subcultured >140 times. CPB cell line predominantly consisted of fibroblast‐like cells that could grow better in Leibovitz's L‐15 supplemented with 10% foetal bovine serum at 28° C. Polymerase chain reaction amplification of 18s recombinant (r)RNA confirmed the origin of this cell line from S. chuatsi. The CPB cell line was cryopreserved at different passage levels and revived successfully with 80–90% survival. The cell line was further characterized by chromosome number and transfection. The CPB cells were highly susceptible to infectious spleen and kidney necrosis virus (ISKNV) with a titre of 6·58–6·62 log TCID50 ml−1 and numerous ISKNV particles were observed in the cytoplasm by transmission electron microscopy. At the same time, ISKNV infection was confirmed by reverse transcriptase polymerase chain reaction, immunodot blot and individual challenge experiments. The development and characterization of a new brain cell line from S. chuatsi were described in this study and it could be used as an in vitro tool for propagation of ISKNV and gene expression studies.
Proton beam therapy for unresectable intrahepatic cholangiocarcinoma
Authors: Ohkawa Ayako , Mizumoto Masashi , Ishikawa Hitoshi , Abei Masato , Fukuda Kuniaki , Hashimoto Takayuki , Sakae Takeji , Tsuboi Koji , Okumura Toshiyuki , Sakurai Hideyuki 
Published: November 2014

AbstractBackground and AimTreatment for unresectable intrahepatic cholangiocarcinoma (ICC) has not been established. The aim of the study was to evaluate the outcome of proton beam therapy (PBT) for patients with unresectable ICC.MethodsUp to 2010, 20 patients (11 males, 9 females, median age 63 years old) with unresectable ICC (two, seven, seven, and four in stages II, IIIA, IIIC, and IV, respectively) were treated with PBT. The largest dimensions of the tumors ranged from 15 to 140 mm (median: 50 mm). The intrahepatic region and lymph nodes received median total proton doses of 72.6 GyE in 22 fractions and 56.1 GyE in 17 fractions, respectively. Four patients received concurrent chemotherapy (tegafur, gimeracil, and oteracil; TS‐1) during PBT. Twelve patients were treated curatively, and eight were treated palliatively because tumors were present outside the irradiation field.ResultsIn the curative group, nine tumors within the irradiated field were controlled in follow‐up of 8.6–62.6 months (median: 20.8 months). Median survival rates in the curative and palliative groups were 27.5 and 9.6 months, respectively, and overall 1‐ and 3‐year survival rates were 82% and 38%, and 50% and 0%, respectively. Eight patients survived for > 2 years, and there was no distant metastasis in five of these patients after 2 years. No severe side‐effects occurred.ConclusionsThe results suggest that long‐term survival can be achieved using PBT for patients with unresectable ICC without distant metastasis. Further studies are required to determine the optimal treatment schedule and best combination of PBT and chemotherapy.
Role of the Different Sexuality Domains on the Sexual Function of Women with Premature Ovarian Failure
Authors: Benetti-Pinto Cristina Laguna , Soares Patrícia Magda , Giraldo Helena Patrícia Donovan , Yela Daniela Angerame 
Published: November 2014

ABSTRACT

Introduction
Women with premature ovarian failure (POF) often manifest complaints involving different aspects of sexual function (SF), regardless of using hormone therapy. SF involves a complex interaction between physical, psychological, and sociocultural aspects. There are doubts about the impact of different complaints on the global context of SF of women with POF.

Aim
To evaluate the percentage of influence of each of the sexuality domains on the SF in women with POF.

Methods
Cross-sectional study with 80 women with POF, matched by age to 80 women with normal gonadal function. We evaluated SF through the “Female Sexual Function Index” (FSFI), a comparison between the POF and control groups using the Mann–Whitney test. Component exploratory factor analysis was used to assess the proportional influence of each domain on the composition of the overall SF for women in the POF group.

Main Outcome Measures
SF was evaluated using FSFI. Exploratory Factor Analysis for components was used to evaluate the role of each domain on the SF of women with POF.

Results
The FSFI score was significantly worse for women with POF, with a decrease in arousal, lubrication, orgasm, satisfaction, and dyspareunia. Exploratory factor analysis of SF showed that the domain with greater influence in the SF was arousal, followed by desire, together accounting for 41% of the FSFI. The domains with less influence were dyspareunia and lubrication, which together accounted for 25% of the FSFI.

Conclusion
Women with POF have impaired SF, determined mainly by changes in arousal and desire. Aspects related to lubrication and dyspareunia complaints have lower determination coefficient in SF. These results are important in adapting the approach of sexual disorders in this group of women.
Changes in the Sexual Function During Pregnancy
Authors: Gałązka Iwona , Drosdzol-Cop Agnieszka , Naworska Beata , Czajkowska Mariola , Skrzypulec-Plinta Violetta 
Published: November 2014

Abstract

Introduction
The physiological changes during each trimester of pregnancy have a significant impact on women's sexual behavior.

Aim
The aim of the work was to assess changes in the sexual function during pregnancy.

Methods
The prospective study encompassed 520 pregnant women aged between 18 and 45, of whom 168 were qualified for the final analysis. The research tool was a purpose-designed research questionnaire and the standardized Female Sexual Function Index.

Main Outcome Measures
To assess changes in the sexual function among pregnant women aged 18–45 in the three pregnancy trimesters.

Results
All the studied parameters, i.e., desire, arousal, lubrication, orgasm, satisfaction, and pain, decreased significantly with the progression of pregnancy. Analyzing the frequency of sexual intercourse in the studied group before and during pregnancy, a statistically significant decrease (P &lt; 0.000001) was observed. Sexual desire changed statistically significantly (P = 0.0004). The direction of change concerned decreased sexual desire in the three trimesters compared with the situation before pregnancy. Statistical significance was demonstrated for: decreased sexual desire (P = 0.00007), partner's reluctance (P = 0.002), and pregnancy-related changes in appearance (P = 0.03).

Conclusions
Sexual function was compromised and sexual activity decreased as the pregnancy progressed. Changes in the domains of arousal, lubrication, and orgasm were particularly notable in primaparae in the third trimester of pregnancy. Unsatisfying partner relationship was a significant factor affecting the quality of sexual life during pregnancy.
Genetic structure analysis of strains causing citrus canker in Iran reveals the presence of two different lineages of Xanthomonas citri pv. citri pathotype A*
Authors: Pruvost O. , Goodarzi T. , Boyer K. , Soltaninejad H. , Escalon A. , Alavi S. M. , Javegny S. , Boyer C. , Cottyn B. , Gagnevin L. , Vernière C. 
Published: November 2014

West Asia has been recognized as a major centre for the diversification of Xanthomonas citri pv. citri, a citrus quarantine pathogen of considerable economic importance. However, little genotyping data is available mainly due to the paucity of microbial resources in this region. Using a comprehensive strain collection, several genotyping techniques and a pathogenicity assay, the status of strains causing Asiatic citrus canker in Iran, an internationally significant citrus‐producing country, was clarified. All strains were genetically related to X. citri pv. citri pathotype A* (i.e. strains with a host range restricted to Mexican lime and related species) but not to pathotype A (i.e. strains with a wide host range among rutaceous species). The findings were based on discriminant analysis of the principal components of MLVA‐31 data and were further confirmed by pathogenicity data. Two genetically, geographically and pathologically separate groups of strains in Iran were identified. One of the groups had never been previously reported anywhere in the world. A very strong genetic structure was found (RST = 0·938), consistent with their geographical isolation. Strains from these two groups also differed in terms of their type III effector repertoire. The atypical host range of one of these groups could explain why some Iranian strains had previously been mistakenly identified as pathotype A. This study suggests the absence of invasive pathotype A strains in Iran (known as DAPC 1), which account for most of the economically important outbreaks internationally.
Genetic variants of pulmonary SP‐D predict disease outcome of COPD in a Chinese population
Authors: Ou Chih‐Ying , Chen Chiung‐Zuei , Hsiue Tzuen‐Ren , Lin Sheng‐Hsiang , Wang Jiu‐Yao 
Published: November 2014

AbstractBackground and objectiveAlthough surfactant protein‐D (SP‐D) has been suggested as a biomarker for chronic obstructive pulmonary disease (COPD), the relationship between genetic variants of SP‐D and disease outcome of COPD remains unknown. We hypothesized that genetic polymorphisms of SP‐D are associated with COPD‐related phenotypes and disease prognosis.MethodsA hospital‐based, case‐controlled study was conducted prospectively. Six single nucleotide polymorphisms of the SFTPD gene were determined for genetic association analysis. Inflammatory cytokines and SP‐D serum level were quantified. Frequency of exacerbation and change of lung function were assessed. All‐cause 3‐year mortality was registered.ResultsWe studied 320 smokers (192 with COPD and 128 at‐risk for COPD) who were prospectively monitored for at least 3 years. The serum levels of SP‐D in COPD patients were significantly associated with the degree of airflow obstruction and frequency of exacerbation. Haplotype association analysis revealed that haplotype G‐G‐C‐C‐A was associated with lower risk of COPD (P = 0.03) in our study population. COPD patients with haplotype G‐G‐C‐C‐A had lower serum SP‐D levels (P < 0.001), higher rates of positive response to bronchodilator treatment (P = 0.01), more improvement of forced expiratory volume in 1 s in yearly follow‐up (P = 0.03) and better 3‐year survival rate than COPD patients with non G‐G‐C‐C‐A haplotype (P = 0.03).ConclusionsGenetic haplotype of SP‐D may serve as a valuable prognostic indicator in Chinese patients with COPD.
Is non‐invasive ventilation safe in acute severe asthma?
Authors: Pallin Michael , Hew Mark , Naughton Matthew T. 
Published: November 2014

AbstractBackground and objectiveThe effect of non‐invasive ventilation (NIV) in acute severe asthma is unclear and there are concerns regarding its safety.MethodsWe undertook a 5‐year case–control review of mortality and morbidity associated with NIV use in acute severe asthma and compared this with asthma requiring invasive mechanical ventilation (IMV) and a control group with less severe asthma without ventilatory support.ResultsEight hundred seventy‐three patients had acute severe asthma of whom 30 were treated with NIV, 17 with IMV and 90 served as controls. The mean duration of NIV was 9.5 ± 7.3 h with inspiratory positive airway pressure and expiratory positive airway pressure of 11.9 ± 1.4 and 5.8 ± 1.2 cmH2O respectively. Mortality was zero in the NIV and control groups, compared with 41% in the IMV group. None of the NIV or control groups required escalation to invasive ventilation. There were no instances of haemodynamic compromise in the NIV or control groups. Length of hospital stay was 121 ± 96 h in the NIV group and similar to the severe IMV group (136 ± 99 h, P > 0.05) and significantly longer than the control group (42 ± 40 h, P < 0.05).ConclusionsNIV can be safely used in acute severe asthma although further work is needed to delineate the precise patient selection process.
Circulating tumour cells are linked to plasma D-dimer levels in patients with metastatic breast cancer
Authors: Mego Michal , Zuo Zhuang , Gao Hui , Cohen Evan , Giordano Antonio , Tin Sanda , Anfossi Simone , Jackson Summer , Woodward Wendy , Ueno Naoto , Valero Vicente , Alvarez Ricardo , Hortobagyi Gabriel , Khoury Joseph , Cristofanilli Massimo , Reuben James 
Published: November 2014

SummaryCancer is a risk factor for venous thromboembolism (VTE). Elevated plasma D-dimer and fibrinogen levels are also risk factors for VTE. Furthermore, in patients with metastatic breast cancer (MBC), the presence of circulating tumour cells (CTCs) is a risk factor for VTE. The relationship between CTCs and D-dimer is unknown. The aim of this study was to determine whether CTCs correlate with plasma D-dimer level, fibrinogen level, and risk of VTE in MBC. This prospective study included 47 MBC patients treated from July 2009 through December 2010 at the MD Anderson Cancer Center. CTCs in peripheral blood were detected and enumerated using the CellSearch system. D-dimer and fibrinogen were measured in plasma at the time of CTC detection. Thirty-three patients (70 %) had ≥ 1 CTC, and 22 patients (47 %) had ≥ 5 CTCs. Patients with ≥ 1 CTC or ≥ 5 CTCs had significantly higher mean plasma D-dimer levels (g/mL) than patients with no CTCs and < 5 CTCs (2.48 and 3.31 vs 0.80 and 0.84, respectively; p=0.006 for cut-off ≥ 1 CTC and p=0.003 for cut-off ≥ 5 CTCs). In multivariate analysis, presence of CTCs and number of metastases were positively associated with plasma D-dimer level. CTCs were not associated with plasma fibrinogen level. At median follow-up of 13.5 months, three of 33 patients (9 %) with ≥ 1 CTC had VTE, vs no patients with undetectable CTCs. In conclusion, the presence of CTCs was associated with higher levels of plasma D-dimer in MBC patients. This study further confirms an association between CTCs and risk of VTE.
AS CIÊNCIAS SOCIAIS NAS ESCOLAS DE SAÚDE PÚBLICA
Authors: Mascarenhas Rodolfo dos Santos 
Published: November 2014

A Faculdade de Higiene e Saúde Pública da Universidade de São Paulo, Brasil, foi, se não a primeira, uma das primeiras escolas de saúde pública a introduzir em seu currículo uma disciplina de Ciências Sociais. Criou, em 1945, junto à cadeira de Técnica de Saúde Pública, a disciplina Problemas de Sociologia Aplicada, para alunos do Curso de Higiene e Saúde Pública para médicos. Atualmente, sob a denominação de Ciências Sociais Aplicadas, ministram-se aulas de Sociologia, Antropologia Cultural e noções de Psicologia Social, Política e Economia, a alunos de todos os cursos, nesse instituto universitário. O autor, considerando a importância das ciências sociais para a formação de qualquer técnico em saúde pública, propõe, nas escolas de saúde pública, a criação de uma cátedra especializada nesse setor do conhecimento humano. Tendo verificado que a maior parte das escolas onde se introduziu o ensino de Ciências Sociais, ou vêm dando solução parcial ao problema - com o ensino de uma só disciplina -, ou vêm criando mais de uma cátedra, considera que ambas as orientações não são convenientes, sendo preferível que se estabeleça a unidade de cátedra para as Ciências Sociais Aplicadas. Esta seria integrada pelas seguintes matérias básicas: Antropologia Cultural, Sociologia, Psicologia Social, Economia e Ciência Política. Poderiam também ser incluídas no currículo dos Cursos de Saúde Pública noções gerais de Ecologia Humana, Psicologia, Finanças e Aspectos Filosóficos da Saúde Pública. A cátedra de Ciências Sociais Aplicadas teria as seguintes funções básicas: ensino, pesquisa, orientação de alunos, assessoramento e coordenação. Acredita o autor, baseado em sua experiência - desde 1946 - no ensino de Ciências Sociais na Faculdade de Higiene e Saúde Pública, que o primeiro titular dessa cátedra deve ter formação e experiência em saúde pública e em Ciências Sociais, combinação esta que não é fácil de se encontrar.
Clouds in the Cloud: Weather Forecasts and Applications within Cloud Computing Environments
Authors: Molthan Andrew L. , Case Jonathan L. , Venner Jason , Schroeder Richard , Checchi Milton R. , Zavodsky Bradley T. , Limaye Ashutosh , O’Brien Raymond G. 
Published: November 2014

Abstract
Cloud computing offers new opportunities to the scientific community through cloud-deployed software, data-sharing and collaboration tools, and the use of cloud-based computing infrastructure to support data processing and model simulations. This article provides a review of cloud terminology of possible interest to the meteorological community, and focuses specifically on the use of infrastructure as a service (IaaS) concepts to provide a platform for regional numerical weather prediction. Special emphasis is given to developing countries that may have limited access to traditional supercomputing facilities. Amazon Elastic Compute Cloud (EC2) resources were used in an IaaS capacity to provide regional weather simulations with costs ranging from $40 to $75 per 48-h forecast, depending upon the configuration. Simulations provided a reasonable depiction of sensible weather elements and precipitation when compared against typical validation data available over Central America and the Caribbean.