bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of biomarker panel to predict, prevent and create treatments tailored to the persons with human papillomavirus-induced cervical precancerous lesions
Authors: Lazarenko Liudmyla M , Nikitina Olena E , Nikitin Evgen V , Demchenko Olga M , Kovtonyuk Galyna V , Ganova Larysa O , Bubnov Rostyslav V , Shevchuk Veronika O , Nastradina Natalia M , Bila Viktoria V , Spivak Mykola Ya 
Published: January 2014

Abstract

Introduction
Human papillomavirus (HPV) induce many cancer conditions and cause cervical cancer, second in frequency of malignant disease in women.
The aim was to develop biomarker panel for HPV-induced cervical precancerous diseases in patients infected with herpes simplex virus (HSV).

Material and methods
The study involved 71 women with cervical precancerous diseases (mean age 26 ± 5 years) revealed by colposcopic, cytomorphological, and ultrasound signs which were assessed according to the following: first group, 44 patients infected with HPV; second group, 27 HPV-negative patients; and third group, 30 healthy patients (controls). In cervical specimen, we identified HPV DNA of different oncogenic risk types by polymerase chain reaction (PCR). Enzyme-linked immunosorbent assay (ELISA) kits (JSC SPC ‘DiaprofMed’) were used for detecting antibodies to HSV1 and/or HSV2 and for determining the avidity index. The production of pro-inflammatory cytokines, interferon-γ (IFN-γ), IFN-α, TNF-α, and interleukin-1β (IL-1β), and anti-inflammatory cytokines, IL-4, IL-10, and transforming growth factor-β1 (TGF-β1), were studied by ELISA.

Results
In HPV-induced cervix precancerous diseases, we identified low-avidity IgG antibodies to HSV serum of 20 patients; in the serum of 17 patients, we identified average-avidity antibodies, and high-avidity antibodies were found in 2 patients only. In 14 HPV-negative patients, we found low-avidity IgG antibodies to HSV; in 10 patients, medium avidity. Patients with low-avidity IgG antibodies to herpes virus showed high and medium oncogenic risk HPV types and a decrease of IFN-γ compared to patients with medium-avidity IgG antibodies. Production of IFN-γ was suppressed also in HPV-negative patients with cervical precancers, but we found low- and medium-avidity IgG antibodies to herpes virus. In patients with low-avidity antibodies, we observed increased level of IL-10. Level of IFN-α, IL-1β, IL-2, and IL-4 did not change in patients of all groups, but TGF-β1 increased.

Conclusions
In HPV-positive patients, those with low-avidity IgG antibodies to HSV had immunosuppression, confirmed by increased TGF-β1 and violation of IFN-γ production. Therefore, in pro- and anti-inflammatory cytokines and IgG antibodies to HSV, their avidity is an important diagnostic biomarker of HPV-induced precancerous cervical diseases. Low-avidity IgG antibodies may be an indication for treatment with immunomodulators and antiviral drugs.
Optimization of lead adsorption of mordenite by response surface methodology: characterization and modification
Authors: Turkyilmaz Havva , Kartal Tolga , Yigitarslan Yildiz Sibel 
Published: January 2014

Abstract

Background
In order to remove heavy metals, water treatment by adsorption of zeolite is gaining momentum due to low cost and good performance. In this research, the natural mordenite was used as an adsorbent to remove lead ions in an aqueous solution.

Methods
The effects of adsorption temperature, time and initial concentration of lead on the adsorption yield were investigated. Response surface methodology based on Box-Behnken design was applied for optimization. Adsorption data were analyzed by isotherm models. The process was investigated by batch experiments; kinetic and thermodynamic studies were carried out. Adsorption yields of natural and hexadecyltrimethylammonium-bromide-modified mordenite were compared.

Results
The optimum conditions of maximum adsorption (nearly 84 percent) were found as follows: adsorption time of 85-90 min, adsorption temperature of 50°C, and initial lead concentration of 10 mg/L. At the same optimum conditions, modification of mordenite produced 97 percent adsorption yield. The most appropriate isotherm for the process was the Freundlich. Adsorption rate was found as 4.4. Thermodynamic calculations showed that the adsorption was a spontaneous and an exothermic process.

Conclusions
Quadratic model and reduced cubic model were developed to correlate the variables with the adsorption yield of mordenite. From the analysis of variance, the most influential factor was identified as initial lead concentration. At the optimum conditions modification increased the adsorption yield up to nearly 100 percent. Mordenite was found an applicable adsorbent for lead ions especially in dilute solutions and may also be applicable in more concentrated ones with lower yields.
The association of fasting blood glucose (FBG) and waist circumference in northern adults in Iran: a population based study
Authors: Veghari Gholamreza , Sedaghat Mehdi , Joshaghani Hamidreza , Banihashem Samieh , Moharloei Pooneh , Angizeh Abdolhamid , Tazik Ebrahim , Moghaddami Abbas , Hajian-Tilaki Karimollah , ZahedPasha Yedolla 
Published: January 2014

Abstract
Objectives
The aim of this study was to evaluate the association between Fasting Blood Glucose (FBG) level and Waist Circumference (WC) in men and women among 25–65 years old people in the north of Iran.

Material and methods
This was a cross-sectional and analytical research gender that carried out on the 1797 subjects (941 males and 856 females) between 25–65 years old using multistage cluster sampling technique. FBG was measured in the morning after a 12-hour fast and was determined by using laboratory kits (enzymatic methods) and spectrophotometry technique. Central obesity was defined based on World Health Organization criteria: waist circumference ≥102 cm and ≥88 cm in men and women, respectively. The SPSS.16 software was used for statistical analysis.

Results
As whole, the mean of FBG in women (98.3 ± 40.1 mg/dl) was higher than in men (94.6 ± 32.2 mg/dl). Also, the mean of WC in men 4.5 cm was lower than in women. In men, the mean of FBG statistically differs between normal and central obese subjects both in 35–45 year-age group (P = 0.001) and in 45–55 year-age group (P = 0.042). As whole, in men, the FBG level increased up 2.82 mg/dl in each 10 cm of WC with the highest rate in 35–45 year-age group. In totally, in women, the FBG level increased up 3.48 mg/dl in each 10 cm of WC and in 25–35 year-age group and it was higher than in other age groups. In men, the regression coefficients were constant with age increasing while in women it was decreased. Constant trend in men and decreasing trend in women with age was shown between FBG and WC. The cut-off point of WC for detecting of diabetes obtained 89 cm and 107 cm in men and women, respectively.

Conclusion
The positive correlation was seen between WC and FBG level and it was declined with age in women. Cut-off point for detecting of diabetes in men was less than in women. WC is useable as a predictor of type 2 diabetes mellitus risk among adults in the north of Iran.
Joint effects of mitochondrial DNA 5178 C/A polymorphism and coffee consumption or alcohol consumption on clustering of cardiovascular risk factors in middle-aged Japanese men: a cross-sectional study
Authors: Ito Taku , Kokaze Akatsuki , Ishikawa Mamoru , Matsunaga Naomi , Karita Kanae , Yoshida Masao , Ohtsu Tadahiro , Ochiai Hirotaka , Shirasawa Takako , Nanri Hinako , Hoshino Hiromi , Takashima Yutaka 
Published: January 2014

AbstractBackgroundLongevity-associated mitochondrial DNA 5178 (Mt5178) C/A reportedly modulates the effects of coffee consumption on the risk of hypertension, dyslipidemia and abnormal glucose tolerance, and those of alcohol consumption on the risk of hypertension, dyslipidemia and hyperuricemia in middle-aged Japanese men. However, there has been no research examining whether Mt5178 C/A polymorphism influences the effects of coffee consumption or alcohol consumption on the clustering of cardiovascular risk factors (CRFs).MethodsA total of 332 male subjects (mean age ± SD, 52.8 ± 7.8 years) were selected from among individuals visiting the hospital for regular medical check-ups. After Mt5178 C/A genotyping, a cross-sectional study assessing the joint effects of Mt5178 C/A polymorphism and coffee consumption or alcohol consumption on the clustering of CRFs, namely hypertension, abnormal glucose tolerance, hyper-low-density lipoprotein cholesterolemia, hypo-high density lipoprotein cholesterolemia, hypertriglyceridemia and hyperuricemia, was then conducted.ResultsAfter adjustment for confounding factors, significant and negative associations were observed between coffee consumption and clustering of ≥2 CRFs in subjects with Mt5178C. The adjusted odds ratio (OR) for the clustering of ≥2 or ≥3 CRFs was significantly lower in subjects who consumed 1–3 cups of coffee per day than in those who consumed <1 cup of coffee per day (OR = 0.496, 95% confidence interval (CI): 0.249–0.989, and OR = 0.369, 95% CI: 0.165–0.826, respectively). On the other hand, after adjustment, positive associations between coffee consumption and clustering of ≥2 CRFs were observed in subjects with Mt5178A. However, these associations did not reach a significant level. For Mt5178C genotypic men, the adjusted OR for the clustering of ≥2 or ≥3 CRFs was significantly higher in daily drinkers than in occasional drinkers (OR = 2.737, 95% CI: 1.361–5.502, and OR = 3.024, 95% CI: 1.269–7.210, respectively). On the other hand, the association between Mt5178A genotype and the clustering of ≥2 or ≥3 CRFs did not appear to depend on alcohol consumption.ConclusionsThe present results suggest that Mt5178 C/A polymorphism modifies the effects of coffee consumption or alcohol consumption on the clustering of CRFs in middle-aged Japanese men.
Volume replacement with Ringer-lactate is detrimental in severe hemorrhagic shock but protective in moderate hemorrhagic shock: studies in a rat model
Authors: Hussmann Bjoern , Lendemans Sven , de Groot Herbert , Rohrig Ricarda 
Published: January 2014

Abstract
Introduction
To date, there are insufficient data demonstrating the benefits of preclinically administered Ringer-lactate (RL) for the treatment of hemorrhagic shock following trauma. Recent animal experiments have shown that lactate tends to have toxic effects in severe hemorrhagic shock. This study aimed to compare the effects of RL administered in a rat model of severe hemorrhagic shock (mean arterial blood pressure (MAP): 25 to 30 mmHg) and moderate hemorrhagic shock (MAP: 40 to 45 mmHg).

Methods
Four experimental groups of eight male Wistar rats each (moderate shock with Ringer-saline (RS), moderate shock with RL, severe shock with RS, severe shock with RL) were established. After achieving the specified depth of shock, animals were maintained under the shock conditions for 60 minutes. Subsequently, reperfusion with RS or RL was performed for 30 minutes, and the animals were observed for an additional 150 minutes.

Results
All animals with moderate shock that received RL survived the entire study period, while six animals with moderate shock that received RS died before the end of the experiment. Furthermore, animals with moderate shock that received RL exhibited considerable improvements in their acid-base parameters and reduced organ damage.
In contrast, in animals with severe shock, only two of the animals receiving RS survived but all of the animals receiving RL died early, before the end of the study period. Moreover, the severe shock animals that were treated with RL exhibited considerably worsened acid-base and metabolic parameters.

Conclusions
The preclinical use of RL for volume replacement has different effects depending on the severity of hemorrhagic shock. RL exhibits detrimental effects in cases of severe shock, whereas it has pronounced protective effects in cases of moderate shock.
Seismos
Authors: Liner Christopher L. 
Published: January 2014

My last “Seismos” column showed that an interactive stratigraphic column could be made using Mathematica software (we will call it M from now on) Manipulate function. At the 2013 SEG meeting in Houston, I spoke for a minute with Jim Gaiser, who saw the vision and was discovering all the great things that Manipulate can do. It made me think that a little discussion would be useful. Manipulate has the full power of M's analytical, numerical, and graphical resources behind it and allows you to set parameters via slider bars or other kinds of controllers. This may not sound like much, but it is immensely powerful.
Measuring the Performance of Large-Scale Combinatorial Auctions: A Structural Estimation Approach
Authors: Kim Sang Won , Olivares Marcelo , Weintraub Gabriel Y. 
Published: January 2014

The main advantage of a procurement combinatorial auction (CA) is that it allows suppliers to express cost synergies through package bids. However, bidders can also strategically take advantage of this flexibility, by discounting package bids and “inflating” bid prices for single items, even in the absence of cost synergies; the latter behavior can hurt the performance of the auction. It is an empirical question whether allowing package bids and running a CA improves performance in a given setting. In this paper, we develop a structural estimation approach that estimates the firms' cost structure using bidding data and use these estimates to evaluate the performance of the auction. To overcome the computational difficulties arising from the large number of bids observed in large-scale CAs, we propose a novel simplified model of bidders' behavior based on pricing package characteristics. We apply our method to the Chilean school meals auction, in which the government procures half a billion dollars' worth of meal services every year and bidders submit thousands of package bids. Our estimates suggest that bidders' cost synergies are economically significant in this application (∼5%), and the current CA mechanism achieves high allocative efficiency (∼98%) and reasonable margins for the bidders (∼5%). Overall, this work develops the first practical tool to evaluate the performance of large-scale first-price CAs commonly used in procurement settings. This paper was accepted by Serguei Netessine, operations management.
Medial submuscular plating of the femur in a series of paediatric patients
Authors: Nayagam S. , Davis B. , Thevendran G. , Roche A. J. 
Published: January 2014

We describe the technique and results of medial submuscular plating of the femur in paediatric patients and discuss its indications and limitations. Specifically, the technique is used as part of a plate-after-lengthening strategy, where the period of external fixation is reduced and the plate introduced by avoiding direct contact with the lateral entry wounds of the external fixator pins. The technique emphasises that vastus medialis is interposed between the plate and the vascular structures. A total of 16 patients (11 male and five female, mean age 9.6 years (5 to 17)), had medial submuscular plating of the femur. All underwent distraction osteogenesis of the femur with a mean lengthening of 4.99 cm (3.2 to 12) prior to plating. All patients achieved consolidation of the regenerate without deformity. The mean follow-up was 10.5 months (7 to 15) after plating for those with plates still in situ, and 16.3 months (1 to 39) for those who subsequently had their plates removed. None developed a deep infection. In two patients a proximal screw fractured without loss of alignment; one patient sustained a traumatic fracture six months after removal of the plate. Placing the plate on the medial side is advantageous when the external fixator is present on the lateral side, and is biomechanically optimal in the presence of a femoral defect. We conclude that medial femoral submuscular plating is a useful technique for specific indications and can be performed safely with a prior understanding of the regional anatomy. Cite this article: Bone Joint J 2014;96-B:137–42.
The outcome of surgical treatment for peri-acetabular metastases
Authors: Shahid M. , Saunders T. , Jeys L. , Grimer R. 
Published: January 2014

We reviewed the outcome of patients who had been treated operatively for symptomatic peri-acetabular metastases and present an algorithm to guide treatment. The records of 81 patients who had been treated operatively for symptomatic peri-acetabular metastases between 1987 and 2010 were identified. There were 27 men and 54 women with a mean age of 61 years (15 to 87). The diagnosis, size of lesion, degree of pelvic continuity, type of reconstruction, World Health Organization performance status, survival time, pain, mobility and complications including implant failure were recorded in each case. The overall patient survivorship at five years was 5%. The longest lived patient survived 16 years from the date of diagnosis. The mean survival was 23 months (< 1 to 16 years) and the median was 15 months. At follow-up 14 patients remained alive. Two cementoplasties failed because of local disease progression. Three Harrington rods broke: one patient needed a subsequent Girdlestone procedure. One ‘ice-cream cone’ prosthesis dislocated and was subsequently revised without further problems. We recommend the ‘ice-cream cone’ for pelvic discontinuity and Harrington rod reconstruction for severe bone loss. Smaller defects can be safely managed using standard revision hip techniques. Cite this article: Bone Joint J 2014;96-B:132–6.
The mid-term outcomes of the Oxford Domed Lateral unicompartmental knee replacement
Authors: Weston-Simons J. S. , Pandit H. , Kendrick B. J. L. , Jenkins C. , Barker K. , Dodd C. A. F. , Murray D. W. 
Published: January 2014

Mobile-bearing unicompartmental knee replacements (UKRs) with a flat tibial plateau have not performed well in the lateral compartment, owing to a high dislocation rate. This led to the development of the Domed Lateral Oxford UKR (Domed OUKR) with a biconcave bearing. The aim of this study was to assess the survival and clinical outcomes of the Domed OUKR in a large patient cohort in the medium term.We prospectively evaluated 265 consecutive knees with isolated disease of the lateral compartment and a mean age at surgery of 64 years (32 to 90). At a mean follow-up of four years (sd 2.2, (0.5 to 8.3)) the mean Oxford knee score was 40 out of 48 (sd 7.4). A total of 12 knees (4.5%) had re-operations, of which four (1.5%) were for dislocation. All dislocations occurred in the first two years. Two (0.8%) were secondary to significant trauma that resulted in ruptured ligaments, and two (0.8%) were spontaneous. In four patients (1.5%) the UKR was converted to a primary TKR. Survival at eight years, with failure defined as any revision, was 92.1% (95% confidence interval 81.3 to 100).The Domed Lateral OUKR gives good clinical outcomes, low re-operation and revision rates and a low dislocation rate in patients with isolated lateral compartmental disease, in the hands of the designer surgeons.Cite this article: Bone Joint J 2014;96-B:59–64.
Survival analysis of elderly patients with a fracture of the odontoid peg
Authors: Venkatesan M. , Northover J. R. , Wild J. B. , Johnson N. , Lee K. , Uzoigwe C. E. , Braybrooke J. R. 
Published: January 2014

Fractures of the odontoid peg are common spinal injuries in the elderly. This study compares the survivorship of a cohort of elderly patients with an isolated fracture of the odontoid peg versus that of patients who have sustained a fracture of the hip or wrist. A six-year retrospective analysis was performed on all patients aged > 65 years who were admitted to our spinal unit with an isolated fracture of the odontoid peg. A Kaplan–Meier table was used to analyse survivorship from the date of fracture, which was compared with the survivorship of similar age-matched cohorts of 702 consecutive patients with a fracture of the hip and 221 consecutive patients with a fracture of the wrist. A total of 32 patients with an isolated odontoid fracture were identified. The rate of mortality was 37.5% (n = 12) at one year. The period of greatest mortality was within the first 12 weeks. Time made a lesser contribution from then to one year, and there was no impact of time on the rate of mortality thereafter. The rate of mortality at one year was 41.2% for male patients (7 of 17) compared with 33.3% for females (5 of 15). The rate of mortality at one year was 32% (225 of 702) for patients with a fracture of the hip and 4% (9 of 221) for those with a fracture of the wrist. There was no statistically significant difference in the rate of mortality following a hip fracture and an odontoid peg fracture (p = 0.95). However, the survivorship of the wrist fracture group was much better than that of the odontoid peg fracture group (p < 0.001). Thus, a fracture of the odontoid peg in the elderly is not a benign injury and is associated with a high rate of mortality, especially in the first three months after the injury. Cite this article: Bone Joint J 2014;96-B:88–93.
Are upper labial frenectomies in children aged 11 and under appropriate? Is it time to change practice and agree guidelines?
Authors: Al-Najjim Asseel , Sen Pinaki 
Published: January 2014

A retrospective five-year review of upper labial frenectomies in a hospital in the east of England. AIMS Determine how many patients underwent surgery; indications for surgery; assess awareness of practitioners to local guidelines; compare the results to set standards and recommend change to our practice. METHOD A sample of 41 patients has been reviewed, ranging between 3–48 years old. Female-to-male ratio is 4:1. Children aged 11 years or under represented 20% of the sample. RESULTS The rationale for upper labial frenectomy was varied, possibly owing to a lack of consensus among practitioners regarding the timing of frenectomy. The majority of patients (73%) were between 12–16 years old, of whom 81% were females. 59% of patients were referred for orthodontic reasons and 78% of these had an orthodontic opinion. 88% of referring practitioners were not aware of any guidelines for upper labial frenectomy. CONCLUSIONS National guidelines based on a consensus view will streamline the referral pattern.
Topology and Control of the Cell-Cycle-Regulated Transcriptional Circuitry
Authors: Haase Steven B , Wittenberg Curt 
Published: January 2014

Abstract
Nearly 20% of the budding yeast genome is transcribed periodically during the cell division cycle. The precise temporal execution of this large transcriptional program is controlled by a large interacting network of transcriptional regulators, kinases, and ubiquitin ligases. Historically, this network has been viewed as a collection of four coregulated gene clusters that are associated with each phase of the cell cycle. Although the broad outlines of these gene clusters were described nearly 20 years ago, new technologies have enabled major advances in our understanding of the genes comprising those clusters, their regulation, and the complex regulatory interplay between clusters. More recently, advances are being made in understanding the roles of chromatin in the control of the transcriptional program. We are also beginning to discover important regulatory interactions between the cell-cycle transcriptional program and other cell-cycle regulatory mechanisms such as checkpoints and metabolic networks. Here we review recent advances and contemporary models of the transcriptional network and consider these models in the context of eukaryotic cell-cycle controls.