bluuebunny/crossref_metadata_2025_split huggingface dataset:
Purine-rich Element-binding Protein B Mediates Ferroptosis in Lipopolysaccharide-induced Raw264.7 Macrophage Inflammation
Authors: Wang Zhaosi , Zhang Wei , Zhu Xiangrui , Mei Jian , Wang Xiaoying , Zhang Lixin , Ou Langlin , Guan Xiaoyu , Pang Xiangming , Liu Yuxiang , Meng Zitong , Ma Cui 
Published: August 2024

Abstract
Lipopolysaccharide (LPS) plays an important role in Raw264.7 macrophage ferroptosis and inflammation. Purine-rich element-binding protein B (Purb) influences cellular processes by regulating gene expression as a transcription factor. However, the effect and molecular mechanism of Purb in regulating Raw264.7 macrophage ferroptosis is still unknown. In this study, we used malondialdehyde, glutathione (GSH) assays, Fe2+ fluorescence, reactive oxygen species staining, and western blotting to determine the effect of Purb on LPS-induced Raw264.7 macrophage ferroptosis. Pharmacological inhibitor of ferroptosis was utilized to explore its potential effects for inflammation by reverse transcription-quantitative polymerase chain reaction analysis. Meanwhile, chromatin immunoprecipitation was performed to verify the binding of Purb and the GSH-dependent peroxidase 4 (Gpx4). The results showed that LPS-induced inflammation in Raw264.7 macrophages was inhibited by ferroptosis inhibitor Fer-1 treatment. LPS inhibited the expression of Purb in Raw264.7 macrophages. In addition, Purb overexpression relieved the ferroptosis, and inflammatory response of Raw264.7 macrophages induced by LPS. Mechanistically, the binding of Purb to the Gpx4 promoter was decreased after LPS stimulation. Therefore, we concluded for the first time that Purb played a critical role in LPS-induced ferroptosis and inflammatory response by regulating the transcription of Gpx4. These results provide a theoretical basis for further research on the macrophage ferroptosis and inflammation.
Robotic Rehabilitation in India: A Survey of Attitudes and Perceptions
Authors: Pillai Ijaz N. 
Published: August 2024

Abstract

Introduction:
Robotic rehabilitation is a recent advancement in the field of neurorehabilitation that can benefit patients suffering from traumatic brain injuries, strokes and spinal cord injuries. Despite its availability in India since more than a decade, the number of people who are availing such benefits are a minority of the patient population.

Materials and Methods:
The aim of the study was to understand the trends and perceptions of people from medical and non-medical professions. The objective of the study was to evaluate the awareness levels and general perceptions about robotics in rehabilitation, across people from healthcare and other fields including rehabilitation physicians from various parts of the country, through an open survey and using an 11-point scoring system for objective evaluation. The research work was conducted as an observational survey-based study. Questions related to awareness levels, self-perceived levels of affordability and effectiveness of robotics in the field of rehabilitation were covered through a questionnaire made by the author, completely masking the personal information of the respondents to ensure anonymity.

Results:
A total of 145 people from different parts of the country belonging to both healthcare and other fields responded. Scores were assessed out of 10, in all domains. The results show a significant number of people being completely unaware of robotics in the field of rehabilitation, with a low average awareness score of 3.4 out of 10. This trend was also seen amongst medical professionals. Although the reported levels of affordability were low at 3.6, the average scores of perceived effectiveness were high at 5.9. Most people believed financial limitations and lack of awareness and availability as common barriers of implementation of robotic rehabilitation in a country like India.

Conclusion:
This study shows that despite the availability of advanced resources in major cities in the country, there is a lack of awareness amongst doctors and members from other fields, which may result in potential benefiters not being able to make informed decisions about rehabilitative options. Although doctors of the field of rehabilitation generally had a more positive response, the rest of the members are largely unaware. Improving the availability and accessibility to such advances will help provide equal opportunity of access to clients who could benefit from the same. Upscaling the manufacturing and development of indigenous robots for rehabilitation, amongst other methods may help reduce the expenses on users, thereby improving logistic and financial accessibility and availability.
Depression, anxiety, coping, and resilience among amputees: A cross-sectional tertiary care hospital-based study
Authors: Phutane Prasanna , Santre Manjeet , Tiwari Kumud , Rathi Madhur 
Published: August 2024

Background:
Amputation significantly impacts an individual’s physical and psychological well-being. It is essential to understand these implications of amputation as well as the coping and resilience of amputees as part of comprehensive patient care.

Aim:
To study depression, anxiety, coping, and resilience among tertiary care amputee cases.

Materials and Methods:
Consecutive 100 amputees seeking treatment in a tertiary care hospital were included in the study with their consent after ethics committee approval. The Hospital Anxiety and Depression Scale (HADS), Brief Resilience Scale (BRS-6), and Coping Inventory for Stressful Situations (CISS-21) scale were administered. The data were analyzed using the SPSS software with the Chi-square test and Spearman’s correlation.

Results:
Depression affected a significant majority (67%) of patients, while anxiety was present in one-third (33%) of individuals. Certain sociodemographic factors and clinical parameters such as age group, residence, socioeconomic status, side of amputation, and duration of amputation showed statistically significant correlations with depression, anxiety, and low resilience. Emotional coping was positively correlated to anxiety (P < 0.001) and depression (P < 0.001) while task coping was negatively correlated to anxiety (P < 0.001) and depression (P < 0.001). Resilience was negatively correlated to anxiety (P < 0.001) and depression (P < 0.001).

Conclusion:
There exists a significant burden of depression and anxiety in amputees. Those with positive coping and high resilience were better at adjusting to amputation than those with emotion-based coping and low resilience.
Assessment of Stigma among Caregivers of Armed Forces Professionals Suffering from Mental Illness
Authors: Bhattacharya Chandrima , Kumar Sanjay , Sinha Alok Kumar , Ahmad Parvaiz , Ahmad Shafiq , Singh Harpreet , Ahmad Aijaz 
Published: August 2024

Abstract

Background:
Having a person with mental illness in the family invariably leads to stigmatic experiences to the family members. Perceived stigma in caregivers leads to social isolation, psychological distress, and suboptimal functioning. Additionally, perceived stigma among such caregivers is one of the barriers that prevent them from taking appropriate care of their dependents with mental illness. There has been little research in this field in the Indian armed forces population.

Objective:
The objective of this study was to assess the perceived stigma among caregivers of armed forces personnel suffering from mental illnesses.

Methods:
The calculated sample size was 139 but in our study period of time (December 1, 2019–February 28, 2020) we received the enough number of patients and we continued our study with 150 study subjects which is enough more than the 139. Hence, the total number of 150 caregivers visiting the psychiatry outpatient department of a tertiary care military hospital in North India along with their mentally ill dependents were included in the study after obtaining valid informed consent. The subjects were interviewed, their sociodemographic data were collected, and stigma scores were assessed on a 15-item Explanatory Model Interview Catalogue (EMIC) Stigma Scale.

Results:
Total stigma score ranged from 20 to 40 with a median of 31. Caregivers with lower level of education, lower income, rural residency, elder age, and diagnosis of psychosis/substance abuse in dependent were found to have higher scores of perceived stigma.

Conclusions:
Caregivers of patients with mental illnesses experience significant stigma which needs to be addressed so that they can provide optimum care to their mentally ill dependents.

Limitation:
As EMIC does not provide any standard cutoff for low or high stigma or to categorize persons as having stigma or not, it was difficult to compare results with other studies that used different tools to assess stigma.
A Cross-sectional Study to Assess the Patient Satisfaction in the Polyclinic Complex of a Tertiary Care Teaching Hospital
Authors: Gokul Radhakrishnan Venkatesh , Sahran Deepti , Patel Neelesh , Kaur Balpreet , Aggarwal Anuj 
Published: August 2024

Abstract

Introduction:
Assessment of patient satisfaction is a commonly used indicator for measuring the quality of health care. This study aimed to assess patient satisfaction, identify system gaps, and provide recommendations to improve patient satisfaction.

Materials and Methods:
It is a cross-sectional study which employed a prevalidated “Patient Satisfaction Questionnaire-18” (PSQ-18) developed by RAND Corporation. Data collection spanned 4 weeks, involving 332 participants selected through simple random sampling. The analysis included descriptive statistics and scoring based on the Likert scale. Scoring focused on seven dimensions of patient satisfaction, and classification into satisfied, neutral, or dissatisfied categories was guided by guidelines provided by the PSQ-18.

Results:
The study revealed an overall patient satisfaction rate of 72%, with variations across dimensions. The highest satisfaction was observed in general satisfaction (86%).

Conclusion:
The findings aligned with global trends, emphasizing the importance of patient satisfaction in evaluating health-care quality. Continuous evaluations and the implementation of recommendations contribute to improved satisfaction and enhanced health-care quality.
Non-linear relationship between age and subfoveal choroidal thickness in Chinese patients with proliferative diabetic retinopathy
Authors: Lei Chun-Yan , Xie Jiang-Ying , Ran Qi-Bo , Zhang Mei-Xia 
Published: August 2024

BACKGROUND
No study has investigated the change regularity between age and subfoveal choroidal thickness (SFCT) in proliferative diabetic retinopathy (PDR).
AIM
To investigate the relationship between the SFCT and age in Chinese patients with PDR.
METHODS
This was a cross-sectional retrospective study. The participants were hospitalized individuals with type 2 diabetes who underwent vitrectomy for PDR. Con-tralateral eyes that met the criteria were included in the study. All necessary laboratory tests were performed at the time of admission. Central macular thickness (CMT) and SFCT were two quantitative assessments made using enhanced depth imaging optical coherence tomography. CMT was measured automatically and SFCT was measured manually with digital calipers provided by the Heidelberg Eye Explorer software.
RESULTS
The final analysis included a total of 234 individuals with PDR. The average age was 55.60 years old ± 10.03 years old, and 57.69% of the population was male. Univariate analysis revealed a significant negative connection between age and SFCT in patients with PDR [β = -2.44, 95% confidence interval (95%CI): -3.46 to -1.42; P < 0.0001]. In the fully adjusted model, the correlation between SFCT and age remained steady (β = -1.68, 95%CI: -2.97 to -0.39; P = 0.0117). Spline smoothing showed that the relationship between SFCT and age in patients with PDR was non-linear, with an inflection point at 54 years of age.
CONCLUSION
Our findings suggest that age is a key determinant of choroidal thickness. The non-linear link between SFCT and age in PDR patients should be taken into account.
Corilagin alleviates podocyte injury in diabetic nephropathy by regulating autophagy via the SIRT1-AMPK pathway
Authors: Lou Yu , Luan Yu-Ting , Rong Wen-Qing , Gai Yun 
Published: August 2024

BACKGROUND
Diabetic nephropathy (DN) is the most frequent chronic microvascular consequence of diabetes, and podocyte injury and malfunction are closely related to the development of DN. Studies have shown that corilagin (Cor) has hepatoprotective, anti-inflammatory, antibacterial, antioxidant, anti-hypertensive, anti-diabetic, and anti-tumor activities.
AIM
To explore the protective effect of Cor against podocyte injury in DN mice and the underlying mechanisms.
METHODS
Streptozotocin and a high-fat diet were combined to generate DN mice models, which were then divided into either a Cor group or a DN group (n = 8 in each group). Mice in the Cor group were intraperitoneally injected with Cor (30 mg/kg/d) for 12 wk, and mice in the DN group were treated with saline. Biochemical analysis was used to measure the blood lipid profiles. Hematoxylin and eosin staining was used to detect pathological changes in kidney tissue. Immunohistochemistry and Western blotting were used to assess the protein expression of nephrin and podocin. Mouse podocyte cells (MPC5) were cultured and treated with glucose (5 mmol/L), Cor (50 μM), high glucose (HG) (30 mmol/L), and HG (30 mmol/L) plus Cor (50 μM). Real-time quantitative PCR and Western blotting were performed to examine the effects of Cor on podocyte autophagy.
RESULTS
Compared with the control group, the DN mice models had increased fasting blood glucose, glycosylated hemoglobin, triglycerides, and total cholesterol, decreased nephrin and podocin expression, increased apoptosis rate, elevated inflammatory cytokines, and enhanced oxidative stress. All of the conditions mentioned above were alleviated after intervention with Cor. In addition, Cor therapy improved SIRT1 and AMPK expression (P < 0.001), inhibited reactive oxygen species and oxidative stress, and elevated autophagy in HG-induced podocytes (P < 0.01).
CONCLUSION
Cor alleviates podocyte injury by regulating autophagy via the SIRT1-AMPK pathway, thereby exerting its protective impact on renal function in DN mice.
Knowledge and Perceptions about Diagnosis, Clinical Management, and Prevention of Dengue Fever among Physicians during the 2023 Outbreak: A Cross-Sectional Study in Peru
Authors: Araoz-Salinas Julieta M. , Ortiz-Saavedra Brando , Soriano-Moreno Anderson N. , Reategui-Garcia Martín E. , Quispe-Vicuña Carlos , Murrieta-Ruiz Valentina , De-Los-Rios-Pinto Abraham , Benito-Vargas Raysa M. , Sangster-Carrasco Lucero , Morocho-Alburqueque Noelia , Ponce-Rosas Linda , Alave Jorge , Gonzales-Zamora Jose A. 
Published: August 2024

ABSTRACT.
The objective of this study was to evaluate the knowledge level and perception of dengue fever management among Peruvian physicians and to determine the factors associated with higher knowledge. We conducted an analytical cross-sectional study based on an online survey. To evaluate the factors associated with a high level of knowledge (≥70% of correct answers), we used crude (cPR) and adjusted (aPR) prevalence ratios by the Poisson regression model. Of 359 respondents (median age: 33 years; male: 56.5%), 78.8% achieved a high level of knowledge. Multivariable analysis showed an independent association with having read the Peruvian clinical practice guidelines for dengue management (aPR: 1.29; 95% CI: 1.12–1.49), having experience in treating patients (aPR: 1.32; 95% CI: 1.03–1.68), and having treated cases frequently (aPR: 1.22; 95% CI: 1.02–1.46). Residing in the eastern macroregion (aPR: 0.83; 95% CI: 0.71–0.97) was associated with a low level of knowledge. In conclusion, Peruvian physicians had a high level of knowledge about dengue fever. This was associated with having clinical experience in dengue management. However, given the low level of knowledge in the eastern macroregion, educational campaigns are necessary in this area.
Case Report: Campylobacter Bacteremia in India
Authors: Meda Srived , Shenoy Padmaja , Kumar Gauri , Varma Muralidhar , Mukhopadhyay Chiranjay , Kalwaje Eshwara Vandana 
Published: August 2024

ABSTRACT.
Gastroenteritis is the frequent clinical manifestation of infection by Campylobacter spp., whereas invasive infections such as bacteremia are less commonly reported. We report 12 cases of Campylobacter bacteremia from a tertiary-care hospital in India and describe their relatively unique characteristics compared with the previously published reports. These cases were detected after the introduction of new blood culture media. The most common symptoms were fever (eight cases, 67%), abdominal pain (six cases, 50%), and vomiting (four cases, 33%), whereas only two cases (17%) had diarrhea. Chronic liver disease was the common underlying condition affecting eight patients (75%). The median Child–Pugh and Model for End-Stage Liver Disease scores were 9 (interquartile range: 7–9.5) and 20 (interquartile range: 15–23) respectively. Campylobacter jejuni was isolated in six patients (50%), two of whom had preceding diarrhea and one patient who had culture evidence of C. jejuni in ascitic fluid. The in-hospital mortality was 8%, but this was due to subsequent Escherichia coli sepsis.
Assessment of Knowledge Management Strategies on the Performance of Kenya Marine and Fisheries Research Institute
Authors: Kioko Josephine 
Published: August 2024

Purpose: The study assessed knowledge management strategies on the performance of Kenya Marine and Fisheries Research Institute. The study objectives were to determine how knowledge acquisition, sharing, storage and expertise (Technology) influenced the performance of Kenya Marine and Fisheries Research Institute.
Methodology: Since the study involved gathering of quantitative and qualitative data, it adopted mixed method approach. The study employed a descriptive survey research design, this was due to the prediction of knowledge management strategies and the performance Kenya Marine and Fisheries Research Institute, in the analysis of different trends, and the formulation of hypotheses that directs future actions. The target population was 168 employees from the Kenya Marine and Fisheries Research Institute. The researcher sampled 118 respondents using Yamane formula (2007) formula, by doing so, the chance of bias in participant selection was reduced and the sample was guaranteed to be representative of the whole population. Data was analyzed using descriptive statistics in the form of means, percentages, and frequencies with the help of Statistical Package for Social Sciences v25 (SPSS V25) software.
Findings: Results were displayed in form of pie chart and tables from which inferences and conclusions were drawn. The findings of the study revealed that knowledge sharing had the most significant influence on the performance of Kenya Marine and Fisheries Research Institute while knowledge acquisition had the least influence. The research finally concluded that knowledge management strategies had a significant effect on the performance of Kenya Marine and Fisheries Research Institute.
Unique Contribution to Theory, Policy and Practice: The study will serve as a resource for researchers and academicians pursuing related research thus contributing to the body of knowledge. The study will be helpful to KMFRI management as it will provide documentation of the strategic leadership role in the implementation of knowledge management strategies thus contributing to identification of areas in which its strategic leadership needs to be strengthened in order to perform better. This study is vibrant in the creation of policies. Policy makers and implementers in the public sector can find this study useful for understanding the stages and analytical processes involved in developing and putting into practice knowledge management plans. In order to ensure that the strategic direction is accomplished and performance is improved, it will enlighten areas that need to be clarified and redefined. Significant contribution to the society will be through generating new knowledge in particular through challenging existing theories and ideas.
THERAPEUTIC BIOLOGICAL ACTIVITIES OF Balanites aegyptiaca (L.) Delile: A REVIEW
Authors: Hamad Saber W , Zahrani Ateeq Ahmed Al , Almalki Hussain D , Bradosty Sarwan W , Fatihah Hasan Nudin  Nur , Anwar Talar K , Mahmood Asaad Mohammed , Dogara Abdulrahman Mahmoud 
Published: August 2024

Medicinal plants continue to be a crucial source of safe, less toxic, economical, available, and reliable natural medicine supplies all over the world. Balanites aegyptiaca Del. (Zygophyllaceae), commonly known as the "desert date", is a thorny shrub or tree found in arid parts of Africa and South Asia. It has traditionally been used to cure a variety of maladies such as wounds, haemorrhoids, jaundice, syphilis, intestinal worm infection, malaria, dysentery, constipation, diarrhoea, stomachaches, asthma, and fever. The fruits of B. aegyptiaca are highly effective as antioxidants, anti-inflammatory, antibacterial, antifungal, antiparasitic, antidiabetic, and anticancer. However, the safety and effectiveness of B. aegyptiaca have not beencompletely studied in humans, so additional well-planned clinical trials are required to corroborate preclinical findings. The WHO has stressed on continued research on medical plants since products derived from plants will continue to be in demand. Searches were conducted on Google Scholar, Science Direct, Google.com, Wiley, PubMed, Hindawi, Springer, and other relevant databases for research publications on B. aegyptiaca. Duplicate publications including thesis papers, and reviews of B. aegyptiaca were excluded This review presents a thorough overview of current information on the therapeutic characteristics of B. aegyptiaca with a focus on its biological activities. It briefly examines its traditional usage, taxonomy, and biological evaluation.
PELATIHAN MANAJEMEN KEUANGAN PADA UMKM DESA BRAJA HARJOSARI KECAMATAN BRAJA SELEBAH KABUPATEN LAMPUNG TIMUR
Authors: Fitri Utami  
Published: August 2024

Abstrak
Tujuan program Pengabdian Kepada Masyarakat (PKM) ini adalah memberikan pengetahuan dan keterampilan mengenai manajemen keuangan kepada pelaku UMKM di Desa Braja Harjosari. Target khusus untuk memberikan pengetahuan dan keterampilan kepada pelaku UMKM dalam pengembangan usaha melalui pelatihan dalam meningkatkan manajemen keuangan. Adapun prospek pengembangan UMKM dikaji berdasarkan pada analisa keuangan. Rencana kegiatan program PKM ini adalah melakukan sosialisasi kepada pelaku UMKM, penyiapan peralatan pelatihan yang menunjang keberhasilan PKM, melakukan bimbingan terhadap pelaku UMKM dalam menyusun laporan keuangan, melakukan pelatihan dan meningkatkan pengetahuan dan keterampilan baik melalui kegiatan-kegiatan lain, serta monitoring dan evaluasi kegiatan untuk menunjang keberhasilan dan keberlanjutan usaha.

Kata Kunci : UMKM, Manajemen Keuangan, Ekonomi Kreatif

Abstract
The purpose of this Community Service (PKM) program is to provide knowledge and skills regarding financial management to MSME actors in Braja Harjosari Village. The specific target is to provide knowledge and skills to MSME actors in business development through training in improving financial management. The prospects for developing MSMEs are assessed based on financial analysis. The PKM program activity plan is to disseminate information to MSME actors, prepare training equipment that supports the success of PKM, provide guidance to MSME actors in preparing financial reports, conduct training and improve knowledge and skills through other activities, as well as monitoring and evaluating activities for support business success and sustainability.

Keywords: MSMEs, Financial Management, Creative Economy