bluuebunny/crossref_metadata_2025_split huggingface dataset:
One‐Pot Biocatalytic Conversion of Chemically Inert Hydrocarbons into Chiral Amino Acids through Internal Cofactor and H2O2 Recycling
Authors: Wang Aiwen , Wang Yongze , You Yuanxiang , Huang Zhiqing , Zhang Xingwang , Li Shengying , Chen Hui 
Published: August 2024

AbstractChemically inert hydrocarbons are the primary feedstocks used in the petrochemical industry and can be converted into more intricate and valuable chemicals. However, two major challenges impede this conversion process: selective activation of C−H bonds in hydrocarbons and systematic functionalization required to synthesize complex structures. To address these issues, we developed a multi‐enzyme cascade conversion system based on internal cofactor and H2O2 recycling to achieve the one‐pot deep conversion from heptane to chiral (S)‐2‐aminoheptanoic acid under mild conditions. First, a hydrogen‐borrowing‐cycle‐based NADH regeneration method and H2O2 in situ generation and consumption strategy were applied to realize selective C−H bond oxyfunctionalization, converting heptane into 2‐hydroxyheptanoic acid. Integrating subsequent reductive amination driven by the second hydrogen‐borrowing cycle, (S)‐2‐aminoheptanoic acid was finally accumulated at 4.57 mM with eep>99 %. Hexane, octane, 2‐methylheptane, and butylbenzene were also successfully converted into the corresponding chiral amino acids with eep>99 %. Overall, the conversion system employed internal cofactor and H2O2 recycling, with O2 as the oxidant and ammonium as the amination reagent to fulfill the enzymatic conversion from chemically inert hydrocarbons into chiral amino acids under environmentally friendly conditions, which is a highly challenging transformation in traditional organic synthesis.
One‐Pot Multienzyme Cascades for Stereodivergent Synthesis of Tetrahydroquinolines
Authors: De Arijit , Shukla Ajeet , Masood Husain Syed 
Published: August 2024

AbstractThe tetrahydroquinoline (THQ) framework is commonly found in natural products and pharmaceutically relevant molecules. Apart from using transition metal catalysts and chiral phosphoric acids, the chiral 2‐substituted 1,2,3,4‐THQs are synthesized using amine oxidase biocatalysts. However, the use of imine reductases (IREDs) in their asymmetric synthesis remained unexplored. In the current work, IREDs are employed in telescopic multienzyme cascades to catalyze the intramolecular reductive amination leading to chiral 2‐alkyl and 2‐aryl substituted‐1,2,3,4‐tetrahydroquinolines starting from inexpensive nitroalkenones. The cascades containing NtDBR (an ene reductase), NfsB (a nitro reductase) with either Na2S2O4 or V2O5, various IREDs, and glucose dehydrogenase (for NADPH regeneration) are used to synthesize a broad range of (R)/(S)‐2‐alkyl‐substituted (THQs) (26 examples) with high yield (up to 93 %) and excellent ee (up to 99 %) in one‐pot. The method further facilitates the one‐pot biocatalytic synthesis of chiral 2‐aryl substituted THQs (26 examples) from amino chalcones. Lastly, the asymmetric synthesis of several (R)‐ and (S)‐THQ based intermediates of Hancock alkaloids showed the practical application of the newly developed biocatalytic cascades.
Lanthanide Complexes Based on Rigid Benzimidazole Carboxylic Acid Ligand: Sensing of Nitrobenzene, Fe(III) Ions, and Adsorption of Dyes
Authors: Yao Xiao‐Yan , Yue Tian‐Cai , Xiong Bin‐Bin , Wang Lu‐Lu , Wang Duo‐Zhi 
Published: August 2024

ABSTRACTFive new lanthanide complexes {[Sm(H2L)(H2O)4(Cl)]⋅H2O⋅Cl}n (1), {[Eu(H2L)(H2O)4Cl]⋅Cl}n (2), {[La(H2L)(H2O)4Cl]⋅Cl}n (3), {[Pr(H2L)(H2O)4Cl]⋅Cl}n (4), {[Ce(H2L)(H2O)4Cl]⋅Cl}n (5) (H3L = 2‐hydroxy‐5‐(5‐(hydroxy‐λ2‐methoxy)‐1H‐benzo[d]imidazole‐5‐carboxylic acid) were successfully synthesized under solvothermal method. 1 exhibits a two‐dimensional (2D) network structure, whereas 2–5 feature one‐dimensional (1D) chain structure. The fluorescence investigations demonstrated that 1–5 could be the fluorescent sensor for nitrobenzene (NB) and Fe(III) ions with high selectivity, sensitivity, and low detection limits. Furthermore, 1–5 displayed the good adsorption to anionic dye Congo red (CR), and the adsorption capacities were 140.5, 206.5, 212.5, 111.5, and 211.5 mg·g−1, respectively.
Guidelines in Practice: Surgical Attire
Authors: Speth Jennifer 
Published: August 2024

ABSTRACTPerioperative team members wear surgical attire when working in the OR. Wearing clean surgical attire is important to maintain a high level of cleanliness for patient safety. The recently updated AORN “Guideline for surgical attire” provides perioperative nurses with recommendations for wearing surgical attire in the semirestricted and restricted areas of the perioperative setting. This article discusses recommendations for laundering; head coverings; shoes; and identification badges, access cards, and personal items. It also includes a scenario describing compliance monitoring for an updated policy related to cleaning cell phones and performing hand hygiene after cell phone use. Perioperative nurses should review the guideline in its entirety and apply the recommendations for wearing surgical attire in the perioperative environment.
Exploring the role of RNASET2 in the immune response of black soldier fly larvae
Authors: Caramella Sara , Baranzini Nicolò , Bruno Daniele , Orlandi Viviana Teresa , Mohamed Amr , Bolognese Fabrizio , Grimaldi Annalisa , Tettamanti Gianluca 
Published: August 2024

AbstractT2 RNases are transferase‐type enzymes distributed across phyla, crucial for breaking down single‐stranded RNA molecules. In addition to their canonical function, several T2 enzymes exhibit pleiotropic roles, contributing to various biological processes, such as the immune response in invertebrates and vertebrates. This study aims at characterizing RNASET2 in the larvae of black soldier fly (BSF), Hermetia illucens, which are used for organic waste reduction and the production of valuable insect biomolecules for feed formulation and other applications. Given the exposure of BSF larvae to pathogens present in the feeding substrate, it is likely that the mechanisms of their immune response have undergone significant evolution and increased complexity. After in silico characterization of HiRNASET2, demonstrating the high conservation of this T2 homolog, we investigated the expression pattern of the enzyme in the fat body and hemocytes, two districts mainly involved in the insect immune response, in larvae challenged with bacterial infection. While no variation in HiRNASET2 expression was observed in the fat body following infection, a significant upregulation of HiRNASET2 synthesis occurred in hemocytes shortly after the injection of bacteria in the larva. The intracellular localization of HiRNASET2 in lysosomes of plasmatocytes, its extracellular association with bacteria, and the presence of a putative antimicrobial domain in the molecule, suggest its potential role in RNA clean‐up and as an alarm molecule promoting phagocytosis activation by hemocytes. These insights contribute to the characterization of the immune response of Hermetia illucens larvae and may facilitate the development of animal feedstuff enriched with highly valuable BSF bioactive compounds.
Identification and Functional Analysis of Adipokinetic Hormone Receptor in Ostrinia furnacalis Guenée Larvae Parasitized by Macrocentrus cingulum
Authors: Wang Libao , Han Zhaoyang , Liu Xu , Li Shuzhong , Bi Honglun , Feng Congjing 
Published: August 2024

ABSTRACTAs a typical G protein‐coupled receptor, the adipokinetic hormone receptor (AKHR) has seven transmembrane domains (TMDs), and its structure and function are similar to the gonadotropin‐releasing hormone receptor (GnRHR) in vertebrates. However, there is a dearth of information on other components of the AKHR signaling pathway and how it functions in the interaction between insect hosts and parasitoids. In this study, we cloned and analyzed the multifunctional Ostrinia furnacalis AKHR (OfAKHR) cDNA (GenBank accession number MF797868). OfAKHR has a 2206 bp full‐length cDNA, which includes an open reading frame containing 1194 bp. OfAKHR contains the typical seven TMDs, and a “DRY” motif. OfAKHR has the highest relative expression in the fat body and the fifth instar larvae. The results revealed that ApoLpⅢ, PPO2, GS, TPS, Cecropin, and Moricin decreased the transcription levels from 48 to 72 h after the knockdown of OfAKHR expression by dsOfAKHR injection in the fourth instar O. furnacalis larvae. The parasitization of Macrocentrus cingulum selectively upregulated the expression levels of nutrition metabolism and immune‐related genes in parasitized O. furnacalis larvae, stimulated lysozyme activity, and obviously raised the concentrations of triglyceride and trehalose in the hemolymph of O. furnacalis larvae. However, they inhibited the activities of PO and trehalase. This study is conducive to a deeper cognition of the roles of OfAKHR in nutrition and immune homeostasis, coevolution, and coexistence between parasitic wasps and hosts. It also sheds light on the potential as the target of pest control reagents.
DNA interaction of selected tetrahydropyrimidine and its effects against CCl4‐induced hepatotoxicity in vivo: Part II
Authors: Milović Emilija , Matić Sanja Lj. , Katanić Stanković Jelena S. , Srećković Nikola , Filipović Ignjat , Bradić Jovana , Petrović Anica , Jakovljević Vladimir , Vazquez Natalia Busto , Janković Nenad 
Published: August 2024

AbstractTetrahydropyrimidine (compound A = methyl 4‐[4′‐(heptyloxy)‐3′‐methoxyphenyl]‐1,6‐dimethyl‐2‐thioxo‐1,2,3,4‐tetrahydropyrimidine‐5‐carboxylate) was chosen for in vivo studies after exhibiting noteworthy in vitro activity against the K562 and MDA‐MB‐231 cell lines, with IC50 values of 9.20 ± 0.14 µM and 12.76 ± 1.93 µM, respectively. According to experimental (fluorescence titration, viscosity, and differential scanning calorimetry) results, A interacts with DNA via the minor groove. In vivo, acute oral toxicity studies in Wistar albino rats proved no noticeable symptoms of either toxicity or death during the follow‐up period. Genotoxic and antigenotoxic studies at three different concentrations of A (5, 10, and 20 mg/kg of body weight) in Wistar albino rats showed that the dose of 5 mg/kg body weight did not cause DNA damage and had a remarkable DNA protective activity against CCl4‐induced DNA damage, with a percentage reduction of 78.7%. It is also important to note that, under the investigated concentrations of A, liver damage is not observed. Considering all experimental outcomes realized under various in vivo investigations (acute oral toxicity, genotoxicity, antigenotoxicity, and biochemical tests), compound A could be a promising candidate for further clinical testing.
Silver‐Catalyzed Carbamoylation and Carbonylative Cyclization of Alkenes with Oxamic Acids
Authors: A Ru‐Han , Bao Zhi‐Peng , Huo Yong‐Wang , Wu Xiao‐Feng 
Published: August 2024

AbstractTransition metal‐catalyzed carbonylation functionalization reaction of alkenes is an attractive research area in modern organic chemistry. However, there have been very few reports on silver‐catalyzed reactions in carbonylation reactions. Herein we developed a silver‐catalyzed carbamoylation and carbonylative cyclization of alkenes with oxamic acids to obtain 2‐acetylamino‐1‐tetralone derivatives. Various desired cyclized products were formed in moderate to good yields through radical intermediates.
Extended state observer‐based trajectory tracking control of a wheeled mobile robot with one unpowered trailer
Authors: Yang Hongjiu , Dang Ran , Li Peng 
Published: August 2024

AbstractIn this paper, trajectory tracking control is investigated for a wheeled mobile robot with one unpowered trailer using an extended state observer (ESO). The unpowered trailer is added to improve load capacity, which results in a large impact on trajectory tracking as a slow‐varying and large disturbance. A backstepping controller is proposed to generate desired velocities in an outer loop of a double closed‐loop structure. The ESO is employed in an inner loop to estimate the slow‐varying and large disturbance from the unpowered trailer. An integral sliding mode controller is also designed in the inner loop to track the desired velocities from the outer loop. Stability analysis for the ESO, the backstepping controller and the integral sliding mode controller is conducted via Lyapunov methods. Simulation results are provided to show the effectiveness of the trajectory tracking control for a wheeled mobile robot with one unpowered trailer.
Extracapsular extension risk assessment using an artificial intelligence prostate cancer mapping algorithm
Authors: Priester Alan , Mota Sakina Mohammed , Grunden Kyla P. , Shubert Joshua , Richardson Shannon , Sisk Anthony , Felker Ely R. , Sayre James , Marks Leonard S. , Natarajan Shyam , Brisbane Wayne G. 
Published: August 2024

AbstractObjectiveThe objective of this study is to compare detection rates of extracapsular extension (ECE) of prostate cancer (PCa) using artificial intelligence (AI)‐generated cancer maps versus MRI and conventional nomograms.Materials and methodsWe retrospectively analysed data from 147 patients who received MRI‐targeted biopsy and subsequent radical prostatectomy between September 2016 and May 2022. AI‐based software cleared by the United States Food and Drug Administration (Unfold AI, Avenda Health) was used to map 3D cancer probability and estimate ECE risk. Conventional ECE predictors including MRI Likert scores, capsular contact length of MRI‐visible lesions, PSMA T stage, Partin tables, and the “PRedicting ExtraCapsular Extension” nomogram were used for comparison.Postsurgical specimens were processed using whole‐mount histopathology sectioning, and a genitourinary pathologist assessed each quadrant for ECE presence. ECE predictors were then evaluated on the patient (Unfold AI versus all comparators) and quadrant level (Unfold AI versus MRI Likert score). Receiver operator characteristic curves were generated and compared using DeLong's test.ResultsUnfold AI had a significantly higher area under the curve (AUC = 0.81) than other predictors for patient‐level ECE prediction. Unfold AI achieved 68% sensitivity, 78% specificity, 71% positive predictive value, and 75% negative predictive value. At the quadrant level, Unfold AI exceeded the AUC of MRI Likert scores for posterior (0.89 versus 0.82, p = 0.003), anterior (0.84 versus 0.80, p = 0.34), and all quadrants (0.89 versus 0.82, p = 0.002). The false negative rate of Unfold AI was lower than MRI in both the anterior (−60%) and posterior prostate (−40%).ConclusionsUnfold AI accurately predicted ECE risk, outperforming conventional methodologies. It notably improved ECE prediction over MRI in posterior quadrants, with the potential to inform nerve‐spare technique and prevent positive margins. By enhancing PCa staging and risk stratification, AI‐based cancer mapping may lead to better oncological and functional outcomes for patients.
Prozessbegleitende Ableitung mechanischer Eigenschaften hochfester Betone bei Schnellfertigung
Authors: Hoppe Jannik , Schwarz Yannik , Stindt Jan , Forman Patrick , Sanio David , Mark Peter 
Published: August 2024

AbstractDie Vorfertigung von Stahlbetonbauteilen bietet erhebliche Vorteile gegenüber einer konventionellen Ortbetonbauweise. Beispielsweise erlaubt die Integration einer rapiden Wärmebehandlung die Reduktion der Fertigungszeit hin zu einer Schnellfertigung in nur wenigen Stunden. Behandlungsdauern und ‐temperaturen nehmen dabei Einfluss auf die Entwicklung der mechanischen Eigenschaften. Die Kontrolle Letzterer gewährleistet die Qualität der Bauteile. Für Betonbauteile werden dazu in der Regel zerstörende Prüfungen in Begleitversuchen eingesetzt. Im Beitrag wird eine Methode entwickelt, mit der sich Druckfestigkeit, E‐Modul und Verbundfestigkeit anhand einer im Prozess integrierbaren Prüfung ableiten lassen. Untersucht wird dazu die Eignung der Rückprallhammerprüfung, wobei die Randbedingungen der Wärmebehandlung berücksichtigt werden. In experimentellen Untersuchungen werden die aufgeführten Zielgrößen für den hochfesten Nanodur‐Beton bei Herstellung mit variierender Wärmebehandlungsdauer bestimmt. Den experimentellen werden rechnerisch, anhand normativ beschriebener Zusammenhänge, abgeleitete Größen gegenübergestellt. So wird ein Modell gebildet, mit dem sich die Druckfestigkeit und auf Basis dieser der Elastizitätsmodul und die Verbundfestigkeit ableiten lassen. Die Methode wird anhand der gegenübergestellten Werte diskutiert und die Anwendung an einem simplen Beispiel gezeigt sowie Empfehlungen für den Einsatz zur Kontrolle mechanischer Eigenschaften in der Schnellfertigung gegeben.
Dissipation pattern and dietary risk assessment of chlorfenapyr and methomyl in corn under Egyptian field conditions
Authors: Saber El‐Sayed , Malhat Farag , Saber Ayman N. , Heikal Sara , Hussien Mohamed 
Published: August 2024

AbstractCorn is the second most widely farmed grain for human consumption. Low corn productivity due to damage caused by pests has led to using pesticides to control pest infestations. However, the uncontrolled application of pesticides on corn harms both environmental and human health. Accordingly, field experiments followed good agricultural practices to investigate the dissipation pattern and terminal residues of chlorfenapyr and methomyl in corn and compare the values with established safety limits. Gas chromatography–tandem mass spectrometer coupled with the quick, easy, cheap, effective, rugged, and safe technique was used to analyze residues of chlorfenapyr and methomyl in corn. The average recoveries varied from 94% to 105%, with relative standard deviations (RSDs) of 8%–13% for chlorfenapyr and from 99% to 111%, with RSDs of 10–16% for methomyl. Chlorfenapyr and methomyl residues degraded in corn following a first‐order kinetic model, with an estimated half‐life (t1/2) of 3.9 and 2.8 days, respectively, and significant degradation (91.4%–98.1.5%, respectively) after 14 days. Although the maximum residue limits of chlorfenapyr and methomyl for corn are yet to be formulated in Egypt, the long‐term dietary risk for those pesticides was acceptable, with arisk quotient < 100%, according to the national assessments. These findings are required to guide the correct and safe application of these insecticides in Egypt.
Use of biliary stents for large coronary arteries
Authors: Madanat Luai , Jabri Ahmad , Sankar Parvathy , Safian Robert D. 
Published: August 2024

AbstractDedicated coronary artery drug‐eluting stents may be inadequate in coronary arteries >6 mm in diameter, due to the risk of stent undersizing if the stent is not fully expanded or to loss of radial strength or damage to the drug coating if the stent is expanded >6 mm. We present two patients with large coronary arteries who were successfully treated with biliary balloon expandable stents.
Laser ablation for preventing coronary obstruction and maintaining coronary access in redo‐TAVR: A proof of concept
Authors: Brlansky John , Qiu Dong , Azadani Ali N. 
Published: August 2024

AbstractBackgroundRedo‐transcatheter aortic valve replacement (TAVR) is a promising treatment for transcatheter aortic valve degeneration, becoming increasingly relevant with an aging population. In redo‐TAVR, the leaflets of the initial (index) transcatheter aortic valve (TAV) are displaced vertically when the second TAV is implanted, creating a cylindrical cage that can impair coronary cannulation and flow. Preventing coronary obstruction and maintaining coronary access is essential, especially in young and low‐risk patients undergoing TAVR. This study aimed to develop a new leaflet modification strategy using laser ablation to prevent coronary obstruction and facilitate coronary access after repeat TAVR.MethodsTo evaluate the feasibility of the leaflet modification technique using laser ablation, the initial phase of this study involved applying a medical‐grade ultraviolet laser for ablation through pericardial tissue. Following this intervention, computational fluid dynamics simulations were utilized to assess the efficacy of the resulting perforations in promoting coronary flow. These simulations played a crucial role in understanding the impact of the modifications on blood flow patterns, ensuring these changes would facilitate the restoration of coronary circulation.ResultsLaser ablation of pericardium leaflets was successful, demonstrating the feasibility of creating openings in the TAV leaflets. Flow simulation results show that ablation of index valve leaflets can effectively mitigate the flow obstruction caused by sinus sequestration in redo‐TAVR, with the extent of restoration dependent on the number and location of the ablated openings.ConclusionsLaser ablation could be a viable method for leaflet modification in redo‐TAVR, serving as a new tool in interventional procedures.
Prevention and treatment of coronary catheter kinking
Authors: Elrayes Mahmoud M. , Al‐Ogaili Ahmed , Jalli Sandeep , Brilakis Emmanouil S. 
Published: August 2024

AbstractCoronary catheter kinking is an uncommon but potentially catastrophic complication of cardiac catheterization. Although simple maneuvers can often result in resolution of a kink, tighter knots may not respond to such measures. We provide a systematic, stepwise approach to the prevention and treatment of catheter kinking.
Cr2O3 Clusters on ZnO as Hydrogen Pools for Efficient Syngas‐To‐Light Olefins
Authors: Ding Beikai , Yang Chengsheng , Chen Yue , Shao Wei‐Peng , Yang Fan , Zhang Chi , Wang Zheng , Liu Yongmei , Cao Yong , Zhu Yifeng , Bao Xinhe 
Published: August 2024

AbstractInterfaces of mixed oxide are often considered the primary catalytic sites, yet the functionalities of the surrounding environments are less understood. Composite oxides, particularly ZnO‐Cr2O3, show high activity and selectivity for syngas conversion, with ZnO dissociating H2 to hydrides and Cr2O3 activating CO. However, hydrides on ZnO cannot remain stable at temperatures above room temperature. Focusing on the puzzlingly high performance despite ZnO's inability to stabilize active hydrides at reaction temperatures (300–400 °C), we clarified the roles of each phase using models of Cr2O3/ZnO and ZnO/Cr2O3. Cr2O3 clusters on ZnO effectively stabilize and store hydrides, significantly enhancing syngas conversion compared to individual oxides or ZnO/Cr2O3. An acetate/ketene pathway on Cr2O3/ZnO was identified, driven by the unique role of Cr2O3 clusters in regulating local hydride and CO coverages, unlike other routes observed on ZnO/Cr2O3. These insights advance the understanding of the active structures and functionalities of mixed oxides in catalysis.