bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnosis of infectious keratitis by mass spectrometry
Authors: HOFFART L , DRANCOURT M , DORNADIN A 
Published: November 2013

AbstractPurpose Microbial keratitis is an infection of the cornea that can be severe since it leads, in some cases, to blindness. One of the most important prognostic factors is the microbiological diagnosis which determines the rapid use of the appropriate treatment. Therefore we sought a faster and more efficient diagnosis method by using mass spectrometry.Methods This study is the analysis of corneal samples with MALDI‐TOF MS as a new approach, undeveloped in the literature, without preparation nor culture of infected epithelia. All together 18 human corneal epithelia were studied to characterize the healthy human corneal spectra. An animal experimentation was also conducted on 45 Balb/c mice for comparison of their left healthy corneas and their right infected corneas by two difficult diagnosis seeds: Mycobacterium chelonae or Acanthamoeba polyphaga.Results Corneal protein spectra can be obtained quickly, in less than ten minutes, and easily by simple deposit on a mass‐spectrometry target. The study of healthy corneas gives reproducible and specific spectra compared to other human tissues available in our database. In animal model, the MALDI‐TOF MS detects an inflammatory response secondary to wound healing corneal and an infectious response to Mycobacterium chelonae.Conclusion This original work consists in using MALDI‐TOF MS for a microbial keratitis analyze directly on infected epithelium. This technique permits, to differentiate easily and quickly, a healthy cornea from an ulcerated or infected one in mice. After the development of microbiologic recognition algorithms, mass‐spectrometry could be used, in the future, for a “minute” diagnosis of infectious keratitis.Diagnosis of infectious keratitis by mass spectrometry.
The use of tear osmolarity as a diagnostic tool for detection of dry eye prior to cataract surgery
Authors: BAUMANN A , COCHENER B 
Published: November 2013

AbstractPurpose To determine if the diagnosis of preoperative dry eye can help to improve outcomes following cataract surgery.Methods This was a prospective that enrolled cataract patients scheduled to undergo standard phacoemulsification surgery followed by placement of an intraocular lens. Patients were assessed for the presence of dry eye before surgery, and then at one week and one month after surgery. Tests used were tear break‐up time (TBUT), Schirmer’s, and tear osmolarity (TearLab Corp, San Diego, CA). All patients were placed on a standard postoperative treatment regime of topical steroids, non‐steroidal anti‐inflammatory drugs (NSAIDs) and antibiotics for one month following surgery.Results Twenty‐five patients were enrolled in the study. The mean preoperative tear osmolarity was 304.6 mOsm/L Twelve patients had preoperative dry eye, defined as a tear osmolarity level greater than 308 mOsm/L. Mean osmolarity remained stable at the 1st postoperative visit.. At the one‐month postoperative visit, the mean osmolarity was 297.4 mOsm/L.Conclusion The results suggest that conventional means of dry eye testing may miss patients with preoperative dry eye, compared to osmolarity testing, which provides an objective measurement. The lower postoperative osmolarity levels seen in this study were no doubt related to the postoperative medication regimen and it would be reasonable to expect that the osmolarity measurement would return to baseline after treatment stopped .
Visual acuity outcomes after Descmet’s stripping automated endothelial keratoplasty compared to penetrating keratoplasty
Authors: MOUNA ALI , PREMY S , AGAPIE A , JEAN‐COLAS A , AMELOOT F , MANEA G , PERONE J 
Published: November 2013

AbstractPurpose To evaluate the difference in visual recovery between Descmet’s Stripping Automated Endothelial Keratoplasty (DSAEK) and Penetrating Keratoplasty (PK).Methods Retrospective cohort study of 42 eyes. 21 eyes underwent PK compared to 21 eyes underwent DSAEK for a Fuchs’ dystrophy or pseudophakic bullous keratopathy. Preoperative and postoperative best spectacle‐corrected visual acuity (BSCVA) was recorded at 1, 3, 6 and 12 month. The PK and DSAEK procedures were performed by the same experienced surgeon with the same donor and similar recipient criteria. All patients underwent a standardized examination that included tonometry, visual acuity (distance and near) and biomicroscopic examination at 1 month, 3 month, 6 month and 12 month. Improvement in BSCVA between each point time was evaluated using paired‐samples t tests.Results The donor and recepient demographics were comparable in the PK and DSAEK groups. In the PK group, the visual recovery was significant after 12 month (1,71 logMAR +/‐ 0,33 to 1,25 logMAR +/‐ 0,58; P=0,023). The mean distance gain was 4,53 lines and the mean near gain was 3,89 lines. In the DSAEK group, the visual recovery was significant after 3 month (1,05 logMAR +/‐ 0,50 to 0,68 logMAR +/‐ 0,38; P=0,025). The mean distance gain was 4,25 lines and the mean near gain was 5,7 lines. The mean distance gain was equivalent between the two groups (P=0,91), and the mean near gain was statistically superior in the DSAEK group (P=0,47).Conclusion There is a faster visual recovery and a more significant near gain after DSAEK surgery for Fuchs’ endothelial dystrophy or pseudophakic bullous keratopathy.
Reproducibility of the thickness of Lasik corneal flaps made by IntraLase FS 60 laser femtosecond (IntraLase, AMO USA) measured by OCT spectral domain (Heidelberg, Germany)
Authors: ABRIEU LACAILLE M , RAMBAUD C , CREPY P , FROUSSART MAILLE F , RIGAL‐SASTOURNE JC 
Published: November 2013

AbstractPurpose The objective of this study was to appreciate the reproducibility of the thickness of Lasik corneal flaps made by the IntraLase FS60 laser femtosecond (IntraLase, AMO USA).Methods It was a retrospective, randomized, double‐blind study.Twenty eight eyes were included in the study. Every flap was program to measure 120 µm. The flaps were measured by Spectral Domain OCT (Heidelberg), one month after surgery in five points on a horizontal line through the center of the pupil (center, 1mm and 2 mm from the center pupil in nasal and temporal) The scheduled and the obtained thicknesses were compared in each point.Results Double reading by two different doctors showed no significant difference in the thickness flap measurements (p<0.3). Average obtained thickness was 6, 84 µm greater than desired thickness (standard deviation of 7, 27 µm), (p<0, 01). The average thickness flap was significantly greater: nasal 2mm: 128µm +/‐7µm, nasal 1mm: 124.4µm+/‐6µm, central: 127.7µm+/‐8µm, temporal 1mm: 125.9µm +/‐7µm, temporal 2mm: 128.2µm +/‐8 µm (p<0.01).Conclusion We have shown in this study that the cutting of a corneal stromal flap during a LASIK procedure is significant thicker with the IntraLase FS60, but the standard deviation is low.
Age‐related changes in central corneal thickness and corneal endothelial characteristics
Authors: GALGAUSKAS S , NORVYDAITė D , STECH S , ASOKLIS R 
Published: November 2013

AbstractPurpose To compare how corneal endothelial cell density, their average size and percentage of regular hexagonal cells depends on age; to estimate the average of endothelial cell density and central corneal thickness in diferent age groups.Methods The data of 211 person (358 eyes), who were examined using a non‐contact specular microscope at the Vilnius University Hospital Santariskiu Clinic Center of Eye Diseases, was analyzed. Parameters examined included corneal endothelial cell density, average size of cells, percentage of regular hexagonal cells, central corneal thickness. Patients age and sex were also noted. All subjects, whose age was from 20 to 89 years, were devided into 7 age groups, each involving 10 years.Results A total of 114 (54,03%) subjects were women and 97 (45,97%) were men.A strong inverse correlation was observed between subject age and corneal endothelial cell density (r= −0.650, p < 0.01), weak inverse correlation between age and central corneal thickness (r= ‐0,156, p< 0,01) and weak direct correlation between cell density and central corneal thickness (r= 0,232, p< 0,01). Average size of cells was directly correlated with age (r= 0,586, p< 0,01). No correlation between age and percentage of regular hexagonal cells was found (p> 0,05).Conclusion Young individuals have higher endothelial cell density, which decreases with age. Corneal thickness also decreases, but its dependence on age is weaker. The lower cell density, the lower corneal thickness. Average size of cells is bigger in young subjects. Percentage of regular hexagonal cells does not depend on age.
Analysis of fungal contamination in contact lens storage cases between 2000 and 2012
Authors: BOUHERAOUA N , TEMSTET C , GOLDSCHMIDT P , VAUDRAN P , LAROCHE L , BORDERIE V , CHAUMEIL C 
Published: November 2013

AbstractPurpose The objective of this study was to identify fungi species isolated in contact lenses boxes from January 2000 to November 2012 and to compare with fungi species found in corneal samples over the same period.Methods Retrospective study from January 2000 to November 2012. Over the same period, were analyzed isolates from 68 patients with clinical signs of keratomycosis confirmed by corneal samples culture. The temporal analysis (over the last 12 years) of the isolates was performed using the chi2 test pattern.Results 456/2433 contact lenses boxes fungi (18.7%). Non‐filamentous fungi (yeasts) were isolated in 280 boxes (61.3%), including 85% of Candida (46% Candida parapsilosis) and 15% of other non‐filamentous fungi. Filamentous fungi were isolated in 200 boxes (43.8%): Fusarium sp. (46.5%), Penicillium (23%), Aspergillus (12%)other filamentous fungi (18.5%). Fusarium sp. were isolated in 51.4% of patients with clinical signs of keratomycosis. A significant increase in filamentous fungi (chi2 = 6.4 p <0.05) could be highlighted in contact lens boxes. Increase in Fusarium sp. in contact lens boxes is significant(chi2 = 17.5 p <0.05) as the number of Fusarium sp. keratomycosis (chi2 = 6.2 p <.05). Contact lens wear remains the main risk factor for keratomycosis (54% of patients).Conclusion Increasing cases of Fusarium sp. kératomycosis must be considered in the development of diagnostic and therapeutic strategies.
Worldwide Eye Banking (WEB) project: International survey of demand and supply
Authors: JULIENNE R , CAMPOLMI N , ALDOSSARY M , THURET G , GAIN P 
Published: November 2013

AbstractPurpose The whole eye banking (EB) process, from corneal retrieval to surgery, is improving. Paradoxically, corneal blindness worldwide is still increasing. The corneal supply is lagging far behind global demand. Few and partial works exist in this field. This has motivated our international survey: the Worldwide Eye Banking (WEB) project. Our final goal is to identify suitable solutions for countries willing to improve their corneal supplyMethods Design: A descriptive epidemiological worldwide transversal study. A questionnaire consisting of 23 items on keratoplasty and EB was designed. The data was collected from EB staff and corneal specialists via e‐mailing, phone calls or direct interviews during international ophthalmology, eye research or EB congressesResults Data was collected from more than 100 countries; covering a world population of 6.5 billions. Approximately 125 000 keratoplasties are performed each year from the 175 000 corneas collected. US EBs, first corneal exporter, use short‐term storage while Europeans use long term organoculture. Lamellar keratoplasty is a new trend. Surgical indications have 2 profiles: infections for undeveloped countries; keratoconus and bullous keratopathy in industrial countriesConclusion This demand/supply disparity is huge in many developing countries. Decreasing demand requires: 1) Trachoma fight in endemic zones 2) Iatrogenic edema prevention. Increasing supply requires: 1) Corneal donation politic dynamism 2) Local eye banking implantation in each country 3) Optimizing storage technique for better efficiency (retrieved/delivered graft ratio) 4) Bioengineering of endothelial graft. No financial interest for all authors
Viable endothelial cell density by triple HEC staining of a failed Descemet stripping automated endothelial keratoplasty
Authors: CAMPOLMI N , GAUTHIER AS , ACQUART S , THEILLIERE C , PEIRERA S , GARRAUD O , HE Z , PISELLI S , THURET G , DELBOSC B , GAIN P 
Published: November 2013

AbstractPurpose The rate of DSAEK failure decreases during learning curve. It has been previously reported that one of the main cause of rapid DSAEK failure was endothelial cell (EC) loss (Zhang, Arch Ophthalmol 2010). Aim: to report a DSAEK immediate failure and its innovative assessment with determination of the viability of residual ECMethods In the eye bank, a cornea (donor: 44 years old, organ‐culture: 23 days, ECD the day before cut: 2963 cells/mm2, deswelling 21h) was precut with a single pass of a 350μm head (Moria microkeratome). Lamella thickness was 199μm (ultrasound). The recipient had a Fuchs dystrophy. In the operating room, trephination with a Baron punch was eccentric but insertion was uneventful. The graft failed despite 2 rebubbling. A penetrating keratoplasty was performed 2 months later. During surgery the explanted lamella was immediately incubated with Hoechst 33342‐Ethidium‐Calcein‐AM (HEC triple staining, Pipparelli, IOVS 2011) to determine the viability of residual EC. The thickness map of the lamella was then performed with an OCT (Casia, Tomey). The posterior surface of the recipient corneal button was observed with a macroscopeResults The eccentric trephination caused a important step on 90° of circumference. Unplanned retention of Descemet membrane with guttae was found on the posterior face of the recipient cornea. The viable ECD of the explanted lamella was 585 cells/mm2. Keratocytes of both the lamella and the recipient cornea were viableConclusion Allogenic EC and keratocytes survive on a detached lamella at least during 2 months in the anterior chamber of the recipient. Grant: AOL 2012
Intrastromal corneal rings and corneal collagen crosslinking for progressive keratoconus: Comparison of 2 sequences
Authors: SAIB N , BONNEL S , ABRIEU M , RIGAL‐SASTOURNE J 
Published: November 2013

AbstractPurpose To compare 2 sequences of combined intrastromal corneal ring segment (ICRS) implantation and corneal collagen crosslinking (CXL) in progressive keratoconus.Methods In this retrospective study, CXL was followed by ICRS implantation (group A) or ICRS implantation was followed by CXL (group B). Uncorrected (UDVA) and corrected (CDVA) distance visual acuities, spherical equivalent (SE), manifest cylinder and mean keratometry (K) were compared preoperatively and postoperatively.Results 17 eyes of 10 patients with progressive keratoconus were included in this study, 11 eyes in group A and 6 in group B. The mean interval between treatments was 4.3+/‐2.9 months in group A and 5+/‐4.4 months in group B. The mean age is 27.6 years in group A and 28.4 years in group B. The 2 groups are equivalent preoperatively. The mean UDVA and CDVA improved in both groups (UDVA:0.50+/‐0.22 to 0.60+/‐0.20, group A, and 0.28+/‐0.19 to 0.62+/‐0.22(p<0.05), group B; CDVA:0.60+/‐0.15 to 0.70+/‐0.14 and 0.70+/‐0.14 to 0.88+/‐0.17 respectively). The cylinder and mean k values decreased in both groups (cylinder ‐3.63D+/‐1.27 to ‐0.95D+/‐1.54 (p<0.05), group A and ‐3.60D+/‐1.85 to ‐2.80D+/‐2.19 group B; mean K:44.8+/‐1.45 to 42.8D+/‐1.7 (p<0.05) and 43.98+/‐1.74 to 43.8D+/‐1.8, respectively). SE: values decreased in group A (‐3.4D+/‐1.5 to ‐1.3D+/‐1.4 (p<0.05)) instead increased in group B (‐1.1D+/‐0.8 to ‐1.7D+/‐1.0). Overall, the 2 sequences observed show an improvement in UDVA, CDVA, cylinder and mean K.Conclusion In our study, there is no statistical difference between the 2 sequences: ICRS implantation followed by CXL or CXL followed by ICRS implantation.
Kyrieleis plaques in herpes zoster virus associated acute retinal necrosis
Authors: KONIDARIS V , BRENT A , VARDARINOS A , DEANE J , EMPESLIDIS T 
Published: November 2013

AbstractPurpose To present a case of unilateral acute retinal necrosis syndrome (ARNS) with associated Kyrieleis plaques.Methods A 56 year old male with recently diagnosed ARNS with necrotic lesions, extensive retinitis 360˚ around the arcades and multifocal lesions presented with decreased vision and floaters in his left eye. Anterior uveitis of 3+ cells, with mutton fat keratic precipitates and vitritis 2+ with severe haze settled down following anti‐viral and steroid treatment. Polymerase chain reaction from anterior chamber tap revealed presence of Herpes Zoster Virus.Results Kyrieleis plaques and segmented periarteritis appeared at presentation and spreaded in the first 2‐3 weeks whilst inflammation was settling down. They affected the majority of retinal arteries near the optic disc; however there was no evidence of sheathing of leakage of the arteries wall on fluorescein angiography.Conclusion Increased doses of steroids appear to control the inflammation. There was no association found between Kyreileis plaques and severity of inflammation.
High doses of fatty acids for reducing cystoid macular oedema secondary to inflammation or branch retinal vein occlusion
Authors: GEORGIOU T , PHOTIOU DMF , NICOLAOU D , NEOKLEOUS A , MICHAEL S , IKONOMOU C 
Published: November 2013

AbstractPurpose To evaluate the effect of high doses EPA/DHA for patients with cystoid macular oedema.Methods 14 eyes with cystoid macular oedema (8 secondary to chronic inflammation and 6 secondary to branch retinal vein occlusion) were treated with 5g/day of EPA/DHA. Visual acuity and macular oedema results from OCT scans were noted.Results The mean macular oedema was reduced from 564um to 243um in 12 months. The mean visual acuity gain at 12 months was 2.85 lines. No anti‐VEGF injections were given during treatment with high doses EPA/DHA.Conclusion Innovative therapy of using high doses of fatty acids to reduce macular oedema and improve vision in patients with cystoid macular oedema secondary to inflammation or branch retinal vein occlusion.
Detection of antibodies against homologous Mycobacterium avium subspecies paratuberculosis and beta‐cell antigen zinc transporter 8 epitopes in Sardinian type 1 diabetic patients with proliferative diabetic retinopathy
Authors: PINNA A , MASALA S , BLASETTI F , MAIORE I , BRUNDU E , COSSU D , PACCAGNINI D , SECHI LA 
Published: November 2013

AbstractPurpose Diabetic retinopathy (DR) is the leading cause of new cases of blindness in developed countries. ZnT8, a beta‐cell membrane protein involved in Zn++ transportation, may act as a major autoantigen in Type 1 Diabetes (T1D). Dysregulation in Zn++ homeostasis has been implicated in the pathogenesis of ischemia in DR. Mycobacterium avium subspecies paratuberculosis (MAP) causes an asymptomatic human infection transmitted from dairy herds through food contamination. MAP3865c, a MAP cell membrane protein, has been shown to display a relevant sequence homology with ZnT8. Moreover, antibodies recognizing MAP3865c epitopes have been found to cross‐react with ZnT8 in T1D patients. The purpose of this study was to detect serum antibodies against 6 highly immunogenic MAP3865c epitopes in T1D patients with proliferative diabetic retinopathy (PDR) and in healthy controls (HCs).Methods Blood samples were obtained from 23 T1D patients with PDR and 39 HCs. Antibodies against 2 trans‐membrane (MAP3865c125–133 MAP3865c133–141) and 4 C terminal (MAP3865c246–252, MAP3865c256–262, MAP3865c261–267 and MAP3865c281–287) peptides were detected by indirect ELISA. Fisher’s exact test and ROC curves were used to assess results.Results Antibodies against MAP3865c peptides were found in 6 out of 23 (26%) T1D patients with PDR and 2 out of 39 (5%) HCs, a statistically significant difference (p=0.04).Conclusion Results suggest that serum antibodies against MAP3865c peptides may play a role in the pathogenesis of PDR in T1D patients.Further larger studies are necessary to confirm these preliminary data.
Risk factors for intraoperative floppy iris syndrome: One year prospective study
Authors: CHATZIRALLI I , PARIKAKIS E , PEPONIS V , MANIATEA A , MITROPOULOS P 
Published: November 2013

AbstractPurpose Intraoperative Floppy Iris Syndrome (IFIS) was associated with a‐blockers use. Our purpose was to evaluate risk factors for IFIS in patients undergoing phacoemulsification.Methods Participants in our study were 1024 patients, who underwent routine phacoemulsification cataract surgery. The following data were recorded and evaluated as possible risk factors for IFIS: ophthalmological conditions (glaucoma, pseudoexfoliation, age‐related macular degeneration), sociodemographic features, axial length, hypertension, diabetes mellitus and medications being taken at the time of surgery. Cases were characterized intraoperatively as IFIS and non‐IFIS. Univariate and multivariate logistic regression was performed with SPSS 19.0.Results IFIS was observed in 61/1024 eyes (5.9%, 95%CI: 4.3%‐7.2%). Current use of tamsulosin, alfuzosin, terazosin, benzodiazepines, quetiapine and finasteride were all independently associated with IFIS. Borderline associations were noted for rivastigmine. Hypertension and short axial length were also significantly associated with IFIS. It is worthy to note that IFIS was not associated with the duration of a‐blockers intake.Conclusion Apart from the well‐established associations with alpha1‐adrenergic receptor antagonists, this prospective study points to benzodiazepines, quetiapine, hypertension and short axial length as potential risk factors for IFIS.
Transporters of glycine, cystine, glutamate and glutamine in canine lens epithelial cells
Authors: OCHIAI H 
Published: November 2013

AbstractPurpose The aim of this study is to evaluate GSH synthesis‐related amino acid (glycine, cystine, glutamate and glutamine) transport activity in a canine lens epithelial cell line (cdLEC) and define the transporter.Methods The primary lens epithelial cells of mature cataract dog were transfected with the expression plasmid DNA of large T antigen from replication origin‐defective simian virus 40 (SV40), then cloned using glass a cylinder. The transport activities of four amino acids in lens epithelial cells were determined. The cDNA sequences of transporter contributing GSH synthesis were also determined.Results Na‐dependent glycine and glutamate transport activity was 2.70 + 0.26 and 4.40 + 1.10 nmol/mg protein min, respectively. The cystine transport was Na‐independent and the transport activity was 0.626 + 0.17 nmol/mg protein min. The cDNA sequence of the glycine transporter 1(GlyT1), cystine/glutamate exchanger (xCT,), Na‐dependent neutral amino acid transporter 2(ASCT2), and glutamate transporter (EAAT) were determined. Western blot analysis confirmed their expression in membrane protein sample of cdLEC.Conclusion Lens epithelial cells possess GlyT1, xCT, ASCT2 and EAAT1‐5.