bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute lymphoblastic leukemia in children with Down syndrome: a retrospective analysis from the Ponte di Legno study group
Authors: Buitenkamp Trudy D. , Izraeli Shai , Zimmermann Martin , Forestier Erik , Heerema Nyla A. , van den Heuvel-Eibrink Marry M. , Pieters Rob , Korbijn Carin M. , Silverman Lewis B. , Schmiegelow Kjeld , Liang Der-Cheng , Horibe Keizo , Arico Maurizio , Biondi Andrea , Basso Giuseppe , Rabin Karin R. , Schrappe Martin , Cario Gunnar , Mann Georg , Morak Maria , Panzer-Grümayer Renate , Mondelaers Veerle , Lammens Tim , Cavé Hélène , Stark Batia , Ganmore Ithamar , Moorman Anthony V. , Vora Ajay , Hunger Stephen P. , Pui Ching-Hon , Mullighan Charles G. , Manabe Atsushi , Escherich Gabriele , Kowalczyk Jerzy R. , Whitlock James A. , Zwaan C. Michel 
Published: November 2013

Key Points
Although the risk of ALL relapse is significantly higher in children with DS, good-prognosis subgroups have been identified. Patients with DS-ALL have higher treatment-related mortality throughout the treatment period independent of the therapeutic regimen.
Replication characteristics of swine influenza viruses in precision-cut lung slices reflect the virulence properties of the viruses
Authors: Meng Fandan , Punyadarsaniya Darsaniya , Uhlenbruck Sabine , Hennig-Pauka Isabel , Schwegmann-Wessels Christel , Ren Xiaofeng , Dürrwald Ralf , Herrler Georg 
Published: November 2013

Abstract
Precision-cut lung slices of pigs were infected with five swine influenza A viruses of different subtypes (A/sw/Potsdam/15/1981 H1N1, A/sw/Bad Griesbach/IDT5604/2006 H1N1, A/sw/Bakum/1832/2000 H1N2, A/sw/Damme/IDT5673/2006 H3N2, A/sw/Herford/IDT5932/2007 H3N2). The viruses were able to infect ciliated and mucus-producing cells. The infection of well-differentiated respiratory epithelial cells by swine influenza A viruses was analyzed with respect to the kinetics of virus release into the supernatant. The highest titres were determined for H3N2/2006 and H3N2/2007 viruses. H1N1/1981 and H1N2/2000 viruses replicated somewhat slower than the H3N2 viruses whereas a H1N1 strain from 2006 multiplied at significantly lower titres than the other strains. Regarding their ability to induce a ciliostatic effect, the two H3N2 strains were found to be most virulent. H1N1/1981 and H1N2/2000 were somewhat less virulent with respect to their effect on ciliary activity. The lowest ciliostatic effect was observed with H1N1/2006. In order to investigate whether this finding is associated with a corresponding virulence in the host, pigs were infected experimentally with H3N2/2006, H1N2/2000, H1N1/1981 and H1N1/2006 viruses. The H1N1/2006 virus was significantly less virulent than the other viruses in pigs which was in agreement with the results obtained by the in vitro-studies. These findings offer the possibility to develop an ex vivo-system that is able to assess virulence of swine influenza A viruses.
Evidence that a tax on sugar sweetened beverages reduces the obesity rate: a meta-analysis
Authors: Cabrera Escobar Maria A , Veerman J Lennert , Tollman Stephen M , Bertram Melanie Y , Hofman Karen J 
Published: November 2013

Abstract

Background
Excess intake of sugar sweetened beverages (SSBs) has been shown to result in weight gain. To address the growing epidemic of obesity, one option is to combine programmes that target individual behaviour change with a fiscal policy such as excise tax on SSBs. This study evaluates the literature on SSB taxes or price increases, and their potential impact on consumption levels, obesity, overweight and body mass index (BMI). The possibility of switching to alternative drinks is also considered.

Methods
The following databases were used: Pubmed/Medline, The Cochrane Database of Systematic Reviews, Google Scholar, Econlit, National Bureau of Economics Research (NBER), Research Papers in Economics (RePEc). Articles published between January 2000 and January 2013, which reported changes in diet or BMI, overweight and/or obesity due to a tax on, or price change of, SSBs were included.

Results
Nine articles met the criteria for the meta-analysis. Six were from the USA and one each from Mexico, Brazil and France. All showed negative own-price elasticity, which means that higher prices are associated with a lower demand for SSBs. Pooled own price-elasticity was -1.299 (95% CI: -1.089 - -1.509). Four articles reported cross-price elasticities, three from the USA and one from Mexico; higher prices for SSBs were associated with an increased demand for alternative beverages such as fruit juice (0.388, 95% CI: 0.009 – 0.767) and milk (0.129, 95% CI: -0.085 – 0.342), and a reduced demand for diet drinks (-0.423, 95% CI: -0.628 - -1.219). Six articles from the USA showed that a higher price could also lead to a decrease in BMI, and decrease the prevalence of overweight and obesity.

Conclusions
Taxing SSBs may reduce obesity. Future research should estimate price elasticities in low- and middle-income countries and identify potential health gains and the wider impact on jobs, monetary savings to the health sector, implementation costs and government revenue. Context-specific cost-effectiveness studies would allow policy makers to weigh these factors.
Omega 3 fatty acids chemosensitize multidrug resistant colon cancer cells by down-regulating cholesterol synthesis and altering detergent resistant membranes composition
Authors: Gelsomino Giada , Corsetto Paola A , Campia Ivana , Montorfano Gigliola , Kopecka Joanna , Castella Barbara , Gazzano Elena , Ghigo Dario , Rizzo Angela M , Riganti Chiara 
Published: November 2013

AbstractBackgroundThe activity of P-glycoprotein (Pgp) and multidrug resistance related protein 1 (MRP1), two membrane transporters involved in multidrug resistance of colon cancer, is increased by high amounts of cholesterol in plasma membrane and detergent resistant membranes (DRMs). It has never been investigated whether omega 3 polyunsatured fatty acids (PUFAs), which modulate cholesterol homeostasis in dyslipidemic syndromes and have chemopreventive effects in colon cancer, may affect the response to chemotherapy in multidrug resistant (MDR) tumors.MethodsWe studied the effect of omega 3 PUFAs docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) in human chemosensitive colon cancer HT29 cells and in their MDR counterpart, HT29-dx cells.ResultsMDR cells, which overexpressed Pgp and MRP1, had a dysregulated cholesterol metabolism, due to the lower expression of ubiquitin E3 ligase Trc8: this produced lower ubiquitination rate of 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGCoAR), higher cholesterol synthesis, higher cholesterol content in MDR cells. We found that DHA and EPA re-activated Trc8 E3 ligase in MDR cells, restored the ubiquitination rate of HMGCoAR to levels comparable with chemosensitive cells, reduced the cholesterol synthesis and incorporation in DRMs. Omega 3 PUFAs were incorporated in whole lipids as well as in DRMs of MDR cells, and altered the lipid composition of these compartments. They reduced the amount of Pgp and MRP1 contained in DRMs, decreased the transporters activity, restored the antitumor effects of different chemotherapeutic drugs, restored a proper tumor-immune system recognition in response to chemotherapy in MDR cells.ConclusionsOur work describes a new biochemical effect of omega 3 PUFAs, which can be useful to overcome chemoresistance in MDR colon cancer cells.
Human immunodeficiency virus type 1 viral protein R (Vpr) induces CCL5 expression in astrocytes via PI3K and MAPK signaling pathways
Authors: Gangwani Mohitkumar R , Noel Richard J , Shah Ankit , Rivera-Amill Vanessa , Kumar Anil 
Published: November 2013

Abstract

Background
Neurocognitive impairments remain prevalent in HIV-1 infected individuals despite current antiretroviral therapies. It is increasingly becoming evident that astrocytes play a critical role in HIV-1 neuropathogenesis through the production of proinflammatory cytokines/chemokines. HIV-1 viral protein R (Vpr) plays an important role in neuronal dysfunction; however, its role in neuroinflammation is not well characterized. The major objective of this study was to determine the effect of Vpr in induction of proinflammatory chemokine CCL5 in astrocytes and to define the underlying mechanism(s).

Methods
SVGA astrocytes were either mock transfected or were transfected with a plasmid encoding HIV-1 Vpr, and the cells were harvested at different time intervals. The mRNA level of CCL5 expression was quantified using real-time RT-PCR, and cell culture supernatants were assayed for CCL5 protein concentration. Immunocytochemistry was performed on HIV-1 Vpr transfected astrocytes to check CCL5 expression. Various signaling mechanisms such as p38 MAPK, PI3K/Akt, NF-κB and AP-1 were explored using specific chemical inhibitors and siRNAs.

Results
HIV-1 Vpr transfected astrocytes exhibited time-dependent induction of CCL5 as compared to mock-transfected astrocytes at both the mRNA and protein level. Immunostained images of astrocytes transfected with HIV-1 Vpr also showed much higher accumulation of CCL5 in comparison to untransfected and mock-transfected astrocytes. Pre-treatment with NF-κB (SC514) and PI3K/Akt (LY294002) inhibitor partially abrogated CCL5 mRNA and protein expression levels as opposed to untreated controls after HIV-1 Vpr transfection. Specific siRNAs against p50 and p65 subunits of NF-κB, p38δ MAPK, Akt-2 and Akt-3, and AP-1 transcription factor substantially inhibited the production of CCL5 in HIV-1 Vpr transfected astrocytes.

Conclusion
These results demonstrate the ability of HIV-1 Vpr to induce CCL5 in astrocytes in a time-dependent manner. Furthermore, this effect was observed to be mediated by transcription factors NF-κB and AP-1 and involved the p38-MAPK and PI3K/Akt pathway.
Phenotypic expression of the p.Leu1077Pro CFTR mutation in Sicilian cystic fibrosis patients
Authors: Parisi Giuseppe Fabio , Cutello Silvia , Di Dio Giovanna , Rotolo Novella , La Rosa Mario , Leonardi Salvatore 
Published: November 2013

Abstract

Background
The p.Leu1077Pro CFTR mutation was firstly described in 1992 as a mild allele that confers a pancreatic sufficiency phenotype but the information collected in database CFTR2 lead to consider p.Leu1077Pro as a severe CF mutation. Although it is typical of Southern Italy, p.Leu1077Pro is not included in the mutation panel firstly tested in individuals originated from this area. The aim of our study was to describe prevalence and clinical features in patients bearing this mutation followed in our Cystic Fibrosis Centre to demonstrate that this mutation should be included in the mutation panel firstly tested in patients originated from Southern Italy.

Findings
We reviewed data from a cohort of 111 cystic fibrosis patients. 4 patients who were heterozygous for the p.Leu1077Pro mutation were included in the study.
In our Cystic Fibrosis Centre, the prevalence of p.Leu1077Pro is 3.6% among all mutations. All patients had positive sweat test values, pancreatic insufficiency and pulmonary exacerbations. One out of four patients even showed both FEV1 and FVC values significantly below the normal range, the presence of bronchiectasis and chronic Pseudomonas aeruginosa colonization.

Conclusions
We found that the p.Leu1077Pro CFTR mutation is associated with a classic CF phenotype confirming what is reported in CFTR2 database. The relatively high prevalence of p.Leu1077Pro associated with the severe clinical course of the disease in patients bearing this mutation is of interest for genetic counselling purposes, as it should be part of mutation panel to be tested in individuals originated from Southern Italy.
Epstein-Barr Virus encoded LMP1 regulates cyclin D1 promoter activity by nuclear EGFR and STAT3 in CNE1 cells
Authors: Xu Yang , Shi Ying , Yuan Qi , Liu Xuli , Yan Bin , Chen Ling , Tao Yongguang , Cao Ya 
Published: November 2013

Abstract
The principal Epstein–Barr virus (EBV) oncoprotein, latent membrane protein 1 (LMP1) is strongly associated with nasopharyngeal carcinoma (NPC), a prevalent cancer in China. The epidermal growth factor receptor (EGFR) is important in carcinogenesis, as it is a ubiquitously expressed receptor tyrosine kinase. Signal transducer and activator of transcription 3 (STAT3) is a master transcriptional regulator in proliferation and apoptosis. Our previous study demonstrated that the nuclear EGFR could bind to the cyclin D1 promoter directly in the presence of LMP1, and the correlation between EGFR and STAT3 in NPC remains to be further explored. Here, we have shown that the interaction of EGFR and STAT3 increased in the nucleus in the presence of LMP1. LMP1 promoted both EGFR and STAT3 binding to the promoter region of cyclin D1, in turn, enhancing the promoter activity of cyclin D1. Furthermore, we demonstrated that both transcriptional activity and mRNA levels of cyclin D1 were decreased by small molecule interference of EGFR and STAT3 activity. These findings may provide a novel linkage between the EGFR and STAT3 signaling pathways and the activation of cyclin D1 by LMP1 in the carcinogenesis of NPC.
Hand hygiene monitoring technology: protocol for a systematic review
Authors: Srigley Jocelyn A , Lightfoot David , Fernie Geoff , Gardam Michael , Muller Matthew P 
Published: November 2013

Abstract

Background
Healthcare worker hand hygiene is thought to be one of the most important strategies to prevent healthcare-associated infections, but compliance is generally poor. Hand hygiene improvement interventions must include audits of compliance (almost always with feedback), which are most often done by direct observation - a method that is expensive, subjective, and prone to bias. New technologies, including electronic and video hand hygiene monitoring systems, have the potential to provide continuous and objective monitoring of hand hygiene, regular feedback, and for some systems, real-time reminders. We propose a systematic review of the evidence supporting the effectiveness of these systems. The primary objective is to determine whether hand hygiene monitoring systems yield sustainable improvements in hand hygiene compliance when compared to usual care.

Methods/Design
MEDLINE, EMBASE, CINAHL, and other relevant databases will be searched for randomized control studies and quasi-experimental studies evaluating a video or electronic hand hygiene monitoring system. A standard data collection form will be used to abstract relevant information from included studies. Bias will be assessed using the Cochrane Effective Practice and Organization of Care Group Risk of Bias Assessment Tool. Studies will be reviewed independently by two reviewers, with disputes resolved by a third reviewer. The primary outcome is directly observed hand hygiene compliance. Secondary outcomes include healthcare-associated infection incidence and improvements in hand hygiene compliance as measured by alternative metrics. Results will be qualitatively summarized with comparisons made between study quality, the measured outcome, and study-specific factors that may be expected to affect outcome (for example, study duration, frequency of feedback, use of real-time reminders). Meta-analysis will be performed if there is more than one study of similar systems with comparable outcome definitions.

Discussion
Electronic and video monitoring systems have the potential to improve hand hygiene compliance and prevent healthcare-associated infection, but are expensive, difficult to install and maintain, and may not be accepted by all healthcare workers. This review will assess the current evidence of effectiveness of these systems before their widespread adoption.

Study registration
PROSPERO registration number: CRD42013004519
Prevention of movement disorders based on somatic abstractions of human movement: Principles, computation and reflection
Authors: Coleman Grisha , Turaga Pavan 
Published: November 2013

Abstract
In this article, we describe a framework for creating technology-based experiential systems rooted in somatic movement education for health and well-being applications. The proposed framework starts with principles derived from somatic modalities in general, and three of the major somatic theories in particular: The Alexander Technique™, The Feldenkrais Method™ and Body-Mind Centering®. In building such a system, we consider ways to translate concepts of expanded motor-sensory awareness, function and ease of movement into measurable attributes for action quality, with simple-to-use and easily available sensing/feedback technology. Inspired by the pioneering work of F. P. Jones, a student of F. M. Alexander, whose research looked ‘to scientifically demonstrate the physiological mechanisms underlying its principles’, our framework seeks to build upon this work by leveraging advances in current technologies for motion capture that enable home-based deployment (KINECT, cell phone accelerometers, etc.), as well as advances in pattern classification technologies for distinguishing actions based on attributes that correlate to subjective, kinaesthetic responses. The framework is intended to provide a foundation in the implementation of a real-time, media-based, interactive experiential system. As well, it is intended to engage communities of somatic practitioners, theorists and technologists in extending the scope of the research and development of new applications.
In the Loop Autoscores: Tensions, tangents and topologies
Authors: Emslie Manny , Spies Sarah 
Published: November 2013

Abstract
The following article is a visual essay that gives access to the creative processes used by The Oblique Strategists (Manny Emslie and Sarah Spies) in Autoscores© In the Loop Project (2012). It can be read in a linear fashion or through an intertextual framework. Inherently, an intertextual approach allows for plurality and a continuous re-arrangement of readings, which we would like to encourage. Embedded within the article are a number of key links to our online scores that make the scores open to possible re-appropriation and performative action. Our self-portraits are included in the visual document below and online at: http://theobliquestrategists.wordpress.com.
Excitable tissues in motion capture practices: The improvising dancer as technogenetic imagist
Authors: Sarco-Thomas Malaika 
Published: November 2013

Abstract
This article outlines the potential of dance improvisation practice to function as a technological interface with one’s environment, drawing parallels between the performances of Twig Dances (Sarco-Thomas 2010) and technologies used in the life sciences to map living matter onto still frames. A postphenomenological approach is used to compare improvisation scores with image-making technologies. Scores that invite corporeal responses to the non-human, and kinaesthetic responses to organic matter, are highlighted as technologies which stand further exploration and examination as they mediate our experience of the world. A diversifying field of somatic practices is proposed as a means to investigate the potential knowledges generated by ‘excitable tissues’ enlivened through improvisational practices.
For a methodology of transformation at the crossroads of somatics and technology: Becoming another …
Authors: Choinière Isabelle 
Published: November 2013

Abstract
This article presents an overview of the motivations behind my artistic approach and a description of current research. My artistic process aims at a transformation of the body based on tactile, kinesthetic and experiential references. It takes the experiential as its raw material. This process inevitably opens up alternative gestural codes, performative modes and behaviours. As a result, it is possible to consider a new relationship between somatics and technology capable of engendering new aesthetic, cognitive and communicational paradigms. Here, technology activates a process that reconfigures sensory-perceptual activity and leads to a change of corporality (corporalité) that in turn generates corporeality (corporéité). This dynamic is based on developing a notion of corporeal potentiality – or interval – and introduces the notion of emergence through which an eventual organization of a new experiential form in the performing arts can appear. Christine Buci-Glucksmann speaks of the interval as the equivalent of the Japanese term Mâ (2003: 89), explaining it as a latent potentiality present within the virtual. I argue that the virtual is part of the physical body and show how these concepts can be linked as the foundations of a methodology that I have developed with dancers. This methodology is one of an evolutive nature and is based on strategies of destabilization of three components of somatics that I have experimented with in order to recreate what Hubert Godard calls ‘an ever fluctuating real’ (Godard in Kuypers 2006: 60).
Geophysical Imaging of Subsurface Earthquake-induced Liquefaction Features at Christchurch Boys High School, Christchurch, New Zealand
Authors: Nobes David C , Bastin Sarah , Charlton Gemma , Cook Rowan , Gallagher Max , Graham Hamish , Grose Daniel , Hedley Joanne , Sharp-Heward Scott , Templeton Sean 
Published: November 2013

On February 22, 2011, a magnitude Mw 6.2 earthquake affected the Canterbury region, New Zealand, resulting in many fatalities. Liquefaction occurred across many areas, visible on the surface as “sand volcanoes”, blisters and subsidence, causing significant damage to buildings, land and infrastructure. Liquefaction occurred at a number of sites across the Christchurch Boys High School sports grounds; one area in particular contained a piston ground failure and an adjacent silt volcano. Here, as part of a class project, we apply near-surface geophysics to image these two liquefaction features and determine whether they share a subsurface connection. Hand auger results enable correlation of the geophysical responses with the subsurface stratigraphy. The survey results suggest that there is a subsurface link, likely via a paleo-stream channel. The anomalous responses of the horizontal loop electromagnetic survey and electrical resistivity imaging highlight the disruption of the subsurface electrical properties beneath and between the two liquefaction features. The vertical magnetic gradient may also show a subtle anomalous response in this area, however the results are inconclusive. The ground penetrating radar survey shows disruption of the subsurface stratigraphy beneath the liquefaction features, in particular sediment mounding beneath the silt ejection (“silt volcano”) and stratigraphic disruption beneath the piston failure. The results indicate how near-surface geophysics allow the characteristics of liquefaction in the subsurface to be better understood, which could aid remediation work following liquefaction-induced land damage and guide interpretation of geophysical surveys of paleoliquefaction features.