bluuebunny/crossref_metadata_2025_split huggingface dataset:
Daily prednisone treatment in duchenne muscular dystrophy in southwest china
Authors: Hu Jun , Ye Yuanzhen , Kong Min , Hong Siqi , Cheng Li , Wang Qiao , Qin Jiaqiang , Zou Lin , Jiang Li 
Published: March 2015

ABSTRACTIntroductionIn this study we aimed to determine the influence of daily prednisone treatment in Duchenne muscular dystrophy (DMD) by performing a prospective, randomized, placebo‐controlled trial in southwestern China.MethodsSixty‐six children with DMD (4–12 years of age) were divided randomly into prednisone and placebo groups. Efficacy and safety of daily prednisone at 0.75 mg/kg/day were evaluated over 12 months by muscle strength and function, quality of life (QoL), quantitative muscle ultrasound (QMUS), and side effects.ResultsSignificant improvements in muscle strength and function, QoL, and QMUS were observed in the prednisone group compared with the placebo‐treated group (P < 0.05). Changes in body weight, height, body mass index, and diastolic blood pressure were similar in both groups (P > 0.05).ConclusionsThis pilot study in southwestern China found that daily prednisone at 0.75 mg/kg/day is suitable for children with DMD. It slowed disease progression and improved QoL and QMUS. Moderate side effects were generally well tolerated. Muscle Nerve 52: 1001–1007, 2015
The linear rheological responses of wedge‐type polymers
Authors: Hu Miao , Xia Yan , Daeffler Christopher S. , Wang Jinhua , McKenna Gregory B. , Kornfield Julia A. , Grubbs Robert H. 
Published: March 2015

ABSTRACTThe linear rheological responses of a series of specially designed wedge‐type polymers synthesized by the polymerization of large molecular weight monomers have been measured. These wedge polymers contained large side groups which contained three flexible branch chains per polymer chain unit. The master curves for these polymers were obtained by time temperature superposition of dynamic data at different temperatures from the terminal flow regime to well below the glass transition temperature, Tg. While these polymers maintained a behavior similar to that of linear polymers, the influence of the large side group structure lead to low entanglement densities and extremely low rubbery plateau modulus values, being near to 13 kPa. The viscosity molecular weight dependence was also somewhat higher than that normally observed for linear polymers, tending toward a power law near to 4.2 rather than the typical 3.4 found in entangled linear chains. The glassy modulus of these branched polymers is also found to be extremely low, being less than 100 MPa at Tg −60 °C. © 2015 Wiley Periodicals, Inc. J. Polym. Sci., Part B: Polym. Phys. 2015, 53, 899–906
Monocyte-Derived Suppressor Cells in Transplantation
Authors: Ochando Jordi , Conde Patricia , Bronte Vincenzo 
Published: March 2015

AbstractMyeloid-derived suppressor cells (MDSC) are cells of myeloid origin with enhanced suppressive function. They are negative regulators of the immune responses and comprise a heterogeneous mixture of immunosuppressive cells of monocytic (M-MDSC) and granulocytic (G-MDSC) origin. A more recent nomenclature proposes the term “suppressive monocyte derived cells” (suppressive MCs) to define CSF1/CSF2-dependent mouse suppressor cells that develop from common monocyte progenitors (cMoPs) after birth. Here, we review the literature about monocytic-derived cells with demonstrated suppressor function in vitro and in vivo within the context of solid organ transplantation.
A web-based collaborative framework for facilitating decision making on a 3D design developing process
Authors: Nyamsuren Purevdorj , Lee Soo-Hong , Hwang Hyun-Tae , Kim Tae-Joo 
Published: March 2015

AbstractIncreased competitive challenges are forcing companies to find better ways to bring their applications to market faster. Distributed development environments can help companies improve their time-to-market by enabling parallel activities. Although, such environments still have their limitations in real-time communication and real-time collaboration during the product development process. This paper describes a web-based collaborative framework which has been developed to support the decision making on a 3D design developing process. The paper describes 3D design file for the discussion that contains all relevant annotations on its surface and their visualization on the user interface for design changing. The framework includes a native CAD data converting module, 3D data based real-time communication module, revision control module for 3D data and some sub-modules such as data storage and data management. We also discuss some raised issues in the project and the steps underway to address them.
A balloon bursting underwater
Authors: Vasel-Be-Hagh A. R. , Carriveau R. , Ting D. S.-K. 
Published: March 2015

A buoyant vortex ring produced by an underwater bursting balloon was studied experimentally. The effect of dimensionless surface tension on characteristics including rise velocity, rate of expansion, circulation, trajectory, and lifetime of the vortex ring bubble was investigated. Results showed reasonable agreement with the literature on vortex rings produced by conventional approaches. It was observed that as the dimensionless surface tension increased, the rise velocity, the circulation and consequently the stability of the vortex ring bubble increased; however, the rate of expansion tends toward constant values. A semi-analytical model is proposed by modifying the drag-based model presented by Sullivan et al. (J. Fluid Mech., vol. 609, 2008, pp. 319–347) to make it applicable to buoyant vortex rings. The modified model suggests that the vortex ring expansion is essentially due to the buoyancy force. An expression is also derived for the circulation in terms of the initial volume of the balloon and the depth at which the balloon bursts.
In vitrocolonic metabolism of coffee and chlorogenic acid results in selective changes in human faecal microbiota growth
Authors: Mills Charlotte E. , Tzounis Xenofon , Oruna-Concha Maria-Jose , Mottram Don S. , Gibson Glenn R. , Spencer Jeremy P. E. 
Published: March 2015

Coffee is a relatively rich source of chlorogenic acids (CGA), which, as other polyphenols, have been postulated to exert preventive effects against CVD and type 2 diabetes. As a considerable proportion of ingested CGA reaches the large intestine, CGA may be capable of exerting beneficial effects in the large gut. Here, we utilise a stirred, anaerobic, pH-controlled, batch culture fermentation model of the distal region of the colon in order to investigate the impact of coffee and CGA on the growth of the human faecal microbiota. Incubation of coffee samples with the human faecal microbiota led to the rapid metabolism of CGA (4 h) and the production of dihydrocaffeic acid and dihydroferulic acid, while caffeine remained unmetabolised. The coffee with the highest levels of CGA (P< 0·05, relative to the other coffees) induced a significant increase in the growth ofBifidobacteriumspp. relative to the control vessel at 10 h after exposure (P< 0·05). Similarly, an equivalent quantity of CGA (80·8 mg, matched with that in high-CGA coffee) induced a significant increase in the growth ofBifidobacteriumspp. (P< 0·05). CGA alone also induced a significant increase in the growth of theClostridium coccoides–Eubacteriumrectalegroup (P< 0·05). This selective metabolism and subsequent amplification of specific bacterial populations could be beneficial to host health.
Responses in gut hormones and hunger to diets with either high protein or a mixture of protein plus free amino acids supplied under weight-loss conditions
Authors: Lobley Gerald E. , Holtrop Grietje , Horgan Graham W. , Bremner David M. , Fyfe Claire , Johnstone Alexandra M. 
Published: March 2015

High-protein diets are an effective means for weight loss (WL), but the mechanisms are unclear. One hypothesis relates to the release of gut hormones by either protein or amino acids (AA). The present study involved overweight and obese male volunteers (n18, mean BMI 36·8 kg/m2) who consumed a maintenance diet for 7 d followed by fully randomised 10 d treatments with three iso-energetic WL diets, i.e. with either normal protein (NP, 15 % of energy) or high protein (HP, 30 %) or with a combination of protein and free AA, each 15 % of energy (NPAA). Psychometric ratings of appetite were recorded hourly. On day 10, plasma samples were taken at 30 min intervals over two consecutive 5 h periods (covering post-breakfast and post-lunch) and analysed for AA, glucose and hormones (insulin, total glucose-dependent insulinotropic peptide, active ghrelin and total peptide YY (PYY)) plus leucine kinetics (first 5 h only). Composite hunger was 16 % lower for the HP diet than for the NP diet (P< 0·01) in the 5 h period after both meals. Plasma essential AA concentrations were greatest within 60 min of each meal for the NPAA diet, but remained elevated for 3–5 h after the HP diet. The three WL diets showed no difference for either fasting concentrations or the postprandial net incremental AUC (net AUCi) for insulin, ghrelin or PYY. No strong correlations were observed between composite hunger scores and net AUCifor either AA or gut peptides. Regulation of hunger may involve subtle interactions, and a range of signals may need to be integrated to produce the overall response.
Reciprocal crosses between two populations ofTrissolcus vassilievi(Mayr) (Hymenoptera: Scelionidae) reveal maternal effects on thermal phenotypes
Authors: Iranipour S. , BenaMolaei P. , Asgari S. , Michaud J.P. 
Published: March 2015

AbstractThe egg parasitoidTrissolcus vassilievi(Mayr) (Hymenoptera: Scelionidae) is a significant natural enemy of the sunn pest,Eurygaster integricepsPuton (Hemiptera: Scutelleridae), the most important pest of wheat in Iran. This study examined the developmental time and egg-to-adult survival of two geographically separate populations ofT. vassilievion two corresponding host populations at five constant temperatures ranging from 15.0 to 35.0±1°C. No wasps of either population emerged at 15.0°C and the temperature threshold for development was similar between populations, ranging from 13.1±0.3 to 13.8±0.4°C for males and 12.2±0.1 to 12.6±0.1°C for females, but the thermal constant varied with gender and parasitoid population. Development of wasps from the colder Tabriz location was slower, with thermal constants for males and females of 172.6±3.1 and 204.1±1.2 degree-days, respectively, compared to Varamin wasps with 164.7±3.0 and 195.6±1.3 degree-days, respectively. Based on genetic inheritance patterns, reciprocal crosses between the two populations were expected to result in females with thermal phenotypes intermediate to their parental populations, and males that resembled their mothers. However, female progeny of crosses more closely resembled their maternal population, indicating a maternal effect on thermal phenotype. Furthermore, the magnitude of the maternal effect on the thermal constant was asymmetric and was more strongly expressed by Varmin than Tabriz females. These results suggest the possibility of using selective crosses between wasp populations, in combination with artificial selection in the laboratory, to tune the thermal phenotype of parasitoids to specific regions prior to augmentative releases.
Host suitability analysis of the bark beetle Scolytus amygdali (Coleoptera: Curculionidae: Scolytinae)
Authors: Zeiri A. , Ahmed M.Z. , Braham M. , Braham M. , Qiu B.-L. 
Published: March 2015

AbstractScolytus amygdali is a polyphagous insect pest that feeds on fruit trees and forest trees. Our study assessed the host preference and reproductive potential of S. amygdali on four tree species: almond (Prunus dulcis), apricot (Prunus armeniaca), peach (Prunus persica), and plum (Prunus domestica). Females of S. amygdali produced maternal galleries that were longer on peach than the other three trees, and female fecundity was highest on peach. Females with longer maternal galleries produced more eggs, indicating a positive correlation between maternal gallery length and female fertility. The under-bark development time of S. amygdali is significantly shorter on plum (45 days) and almond (56 days) than on apricot (65 days) and peach (64 days). Despite this longer development time on peach, our results still suggest that, of the four types of tree tested, peach is the most preferred host for S. amygdali.
Response to conspecific and heterospecific semiochemicals by Sesamia nonagrioides (L.) (Lepidoptera: Noctuidae) gravid females
Authors: Cruz D. , Eizaguirre M. 
Published: March 2015

AbstractThe Mediterranean corn borer, Sesamia nonagrioides, occurs sympatrically in the northeast of Spain with other lepidopteran pests such as Ostrinia nubilalis and Mythimna unipuncta. In this study, we evaluated the electrophysiological and behavioural response of mated and unmated females and males of S. nonagrioides to their own complete pheromone blend, to its own four components separately, and to the pheromone components of the sympatric species O. nubilalis and M. unipuncta. Results of the electroantennogram recordings revealed that females of S. nonagrioides can detect their own pheromone blend and its individual components. Moreover, our results show that unmated females and males of S. nonagrioides are more sensitive to the female pheromone, showing higher electrophysiological response than the mated females and males. Electroantennogram recordings showed that males and females can detect the major sexual pheromone component of O. nubilalis (Z)-11-tetradecenyl acetate and the minor component of the pheromone of M. unipuncta (Z)-9-hexadecenyl acetate. When the sex pheromone stimulus was presented in the dual-choice assays, gravid females of S. nonagrioides were attracted to both their own complete pheromone blend and one of their own minor pheromone components, (Z)-11-hexadecenal, but the major sexual pheromone component of O. nubilalis acts as a behavioural antagonist to the females.
SOVEREIGNTY, SCIENCE, AND CETACEANS: THE WHALING IN THE ANTARCTIC CASE
Authors: Plant Brendan 
Published: March 2015

THE issue of whaling has been the subject of considerable controversy in recent years, as the international community remains divided as to how the world's marine resources should be managed: some states prioritise conservation, while others favour sustainable exploitation. Against this background, Australia initiated proceedings against Japan before the International Court of Justice (ICJ) in May 2010, claiming that Japan's continuing whaling activities, carried out under the guise of scientific research, were in breach of its various obligations under the International Convention for the Regulation of Whaling (ICRW). New Zealand later intervened in the proceedings, exercising its right under Article 63 of the ICJ Statute, and oral pleadings involving the three states were held in June and July 2013. On 31 March 2014, the ICJ delivered its judgment in Whaling in the Antarctic (Australia v Japan, New Zealand Intervening), finding that Japan had violated three provisions of the ICRW – the moratorium on commercial whaling, the ban on factory ships, and the prohibition on whaling in the Southern Ocean Sanctuary – by authorising the killing of certain whale species as part of its JARPA II research programme. The decision has largely been welcomed, especially by environmental activists, for offering a measure of protection to endangered marine life, but the judgment carries broader significance for its treatment of a number of points of international law, including the standard of review exercised by international courts, the role of scientific reasoning in international dispute settlement, and the interpretation of treaties.
Contralateral cochlear implantation prior to vestibular nerve section for ‘drop attacks’ in the only hearing ear
Authors: Shi J , Kertesz T 
Published: March 2015

AbstractBackground:A dilemma occurs in the treatment of second-sided Ménière's disease in the only hearing ear, particularly in patients with severe symptoms such as ‘drop attacks’. This paper describes a patient treated with contralateral cochlear implantation prior to vestibular nerve section of the symptomatic ear.Case report:A 53-year-old man, with second-sided Ménière's disease and drop attacks in the only serviceable right ear, underwent successful left cochlear implantation 30 years after hearing loss, followed by right vestibular nerve section. The patient achieved control of Ménière's attacks and improved hearing. Although the patient experienced oscillopsia post-operatively, he was satisfied with his improved everyday functioning.Conclusion:Patients with severe second-sided Ménière's disease in the only hearing ear are a small but difficult treatment group. In those that are suitable for cochlear implantation in the non-serviceable ear, it is suggested that this be employed prior to surgical treatment of the Ménière's symptoms, even if the implanted ear has had no auditory stimulation for many years.
Bax deficiency extends the survival of Ku70 knockout mice that develop lung and heart diseases
Authors: Ngo J , Matsuyama M , Kim C , Poventud-Fuentes I , Bates A , Siedlak S L , Lee H-g , Doughman Y Q , Watanabe M , Liner A , Hoit B , Voelkel N , Gerson S , Hasty P , Matsuyama S 
Published: March 2015

AbstractKu70 (Lupus Ku autoantigen p70) is essential in nonhomologous end joining DNA double-strand break repair, and ku70−/− mice age prematurely because of increased genomic instability and DNA damage responses. Previously, we found that Ku70 also inhibits Bax, a key mediator of apoptosis. We hypothesized that Bax-mediated apoptosis would be enhanced in the absence of Ku70 and contribute to premature death observed in ku70−/− mice. Here, we show that ku70−/−bax+/− and ku70−/−bax−/− mice have better survival, especially in females, than ku70−/− mice, even though Bax deficiency did not decrease the incidence of lymphoma observed in a Ku70-null background. Moreover, we found that ku70−/− mice develop lung diseases, like emphysema and pulmonary arterial (PA) occlusion, by 3 months of age. These lung abnormalities can trigger secondary health problems such as heart failure that may account for the poor survival of ku70−/− mice. Importantly, Bax deficiency appeared to delay the development of emphysema. This study suggests that enhanced Bax activity exacerbates the negative impact of Ku70 deletion. Furthermore, the underlying mechanisms of emphysema and pulmonary hypertension due to PA occlusion are not well understood, and therefore ku70−/− and Bax-deficient ku70−/− mice may be useful models to study these diseases.
D-2-Hydroxyglutarate does not mimic all the IDH mutation effects, in particular the reduced etoposide-triggered apoptosis mediated by an alteration in mitochondrial NADH
Authors: Oizel K , Gratas C , Nadaradjane A , Oliver L , Vallette F M , Pecqueur C 
Published: March 2015

AbstractSomatic mutations in isocitrate dehydrogenase (IDH)-1 and -2 have recently been described in glioma. This mutation leads to a neomorphic enzymatic activity as the conversion of isocitrate to alpha ketoglutarate (αKG) is replaced by the conversion of αKG to D-2-hydroxyglutarate (D-2HG) with NADPH oxidation. It has been suggested that this oncometabolite D-2HG via inhibition of αKG-dioxygenases is involved in multiple functions such as epigenetic modifications or hypoxia responses. The present study is aimed at deciphering how the mutant IDH can affect cancer pathogenesis, in particular with respect to its associated oncometabolite D-2HG. We show that the overexpression of mutant IDH in glioma cells or treatment with D-2HG triggered an increase in cell proliferation. However, although mutant IDH reduced cell sensitivity to the apoptotic inducer etoposide, D-2HG exhibited no effect on apoptosis. Instead, we found that the apoptotic effect was mediated through the mitochondrial NADH pool reduction and could be inhibited by oxamate. These data show that besides D-2HG production, mutant IDH affects other crucial metabolite pools. These observations lead to a better understanding of the biology of IDH mutations in gliomas and their response to therapy.
Testes-specific protease 50 promotes cell invasion and metastasis by increasing NF-kappaB-dependent matrix metalloproteinase-9 expression
Authors: Song Z B , Ni J-S , Wu P , Bao Y L , Liu T , Li M , Fan C , Zhang W J , Sun L G , Huang Y X , Li Y X 
Published: March 2015

AbstractThe high mortality in breast cancer is often associated with metastatic progression in patients. Previously we have demonstrated that testes-specific protease 50 (TSP50), an oncogene overexpressed in breast cancer samples, could promote cell proliferation and tumorigenesis. However, whether TSP50 also has a key role in cell invasion and cancer metastasis, and the mechanism underlying the process are still unclear. Here we found that TSP50 overexpression greatly promoted cell migration, invasion, adhesion and formation of the stellate structures in 3D culture system in vitro as well as lung metastasis in vivo. Conversely, TSP50 knockdown caused the opposite changes. Mechanistic studies revealed that NF-κB signaling pathway was required for TSP50-induced cell migration and metastasis, and further results indicated that TSP50 overexpression enhanced expression and secretion of MMP9, a target gene of NF-κB signaling. In addition, knockdown of MMP9 resulted in inhibition of cell migration and invasion in vitro and lung metastasis in vivo. Most importantly, immunohistochemical staining of human breast cancer samples strongly showed that the coexpression of TSP50 and p65 as well as TSP50 and MMP9 were correlated with increased metastasis and poor survival. Furthermore, we found that some breast cancer diagnosis-associated features such as tumor size, tumor grade, estrogen receptors (ER) and progesterone receptors (PR) levels, were correlated well with TSP50/p65 and TSP50/MMP9 expression status. Taken together, this work identified the TSP50 activation of MMP9 as a novel signaling mechanism underlying human breast cancer invasion and metastasis.
Nifuroxazide induces apoptosis and impairs pulmonary metastasis in breast cancer model
Authors: Yang F , Hu M , Lei Q , Xia Y , Zhu Y , Song X , Li Y , Jie H , Liu C , Xiong Y , Zuo Z , Zeng A , Li Y , Yu L , Shen G , Wang D , Xie Y , Ye T , Wei Y 
Published: March 2015

AbstractBreast carcinoma is the most common female cancer with considerable metastatic potential. Signal transducers and activators of the transcription 3 (Stat3) signaling pathway is constitutively activated in many cancers including breast cancer and has been validated as a novel potential anticancer target. Here, we reported our finding with nifuroxazide, an antidiarrheal agent identified as a potent inhibitor of Stat3. The potency of nifuroxazide on breast cancer was assessed in vitro and in vivo. In this investigation, we found that nifuroxazide decreased the viability of three breast cancer cell lines and induced apoptosis of cancer cells in a dose-dependent manner. In addition, western blot analysis demonstrated that the occurrence of its apoptosis was associated with activation of cleaved caspases-3 and Bax, downregulation of Bcl-2. Moreover, nifuroxazide markedly blocked cancer cell migration and invasion, and the reduction of phosphorylated-Stat3Tyr705, matrix metalloproteinase (MMP) MMP-2 and MMP-9 expression were also observed. Furthermore, in our animal experiments, intraperitoneal administration of 50 mg/kg/day nifuroxazide suppressed 4T1 tumor growth and blocked formation of pulmonary metastases without detectable toxicity. Meanwhile, histological and immunohistochemical analyses revealed a decrease in Ki-67-positive cells, MMP-9-positive cells and an increase in cleaved caspase-3-positive cells upon nifuroxazide. Notably, nifuroxazide reduced the number of myeloid-derived suppressor cell in the lung. Our data indicated that nifuroxazide may potentially be a therapeutic agent for growth and metastasis of breast cancer.