bluuebunny/crossref_metadata_2025_split huggingface dataset:
Second world record for Barathronus roulei Nielsen, 2019 (Ophidiiformes, Bythitidae), from the Porcupine Bank (Northeast Atlantic)
Authors: Bañón Rafael , de Carlos Alejandro , Comesaña Ángel Sebastián , Barreiro Vázquez José Daniel , Baldó Francisco 
Published: July 2024

AbstractBarathronus is a genus of blind cusk eels comprising 11 valid species. In this paper, we report the second specimen ever documented of Barathronus roulei (Bythitidae) obtained from the Porcupine Bank by R.V. Vizconde de Eza using a bottom trawl at a depth of 1349 m. Morphological description and illustrations, including a radiograph, are provided. In addition, three new sequences corresponding to three different genes, cytochrome c oxidase subunit I (COI)‐DNA barcoding, 16S ribosomal RNA (16S), and recombination activating protein 1 (RAG1), have been added to the molecular repositories, representing the first sequences for the species.
Ontogenic transformation of the ankle from the initial mediolateral arrangement of the calcaneus and talus: A histological study of human embryos and early fetuses
Authors: Kim Ji Hyun , Ishizuka Satoshi , Kitamura Kei , Murakami Gen , Rodríguez‐Vázquez José Francisco , Abe Shin‐ichi , Kasahara Masataka 
Published: July 2024

AbstractThe human calcaneus is robust and provides a prominent heel for effective bipedal locomotion, although the adjacent talus has no muscle attachments. However, there is incomplete information about the morphological changes in these prominent bones during embryo development. We examined serial histological sections of 23 human embryos and early‐term fetuses (approximately 5–10 weeks' gestational age [GA]). At a GA of 5 weeks, the precartilage talus was parallel to and on the medial side of the calcaneus, which had a prolate spheroid shape and consisted of three masses. At a GA of 6 weeks, the cartilaginous talus extended along the proximodistal axis, and the tuber calcanei became long and bulky, with a small sustentaculum talus at the “distal” side. At a GA of 6 to 8 weeks, the sustentaculum had a medial extension below the talus so that the talus “rode over” the calcaneus. In contrast, the talus had a more complex shape, depending on the growth of adjacent bones. At a GA of 9 to 10 weeks, the talus was above the calcaneus, but the medial part still faced the plantar subcutaneous tissue because of the relatively small sustentaculum. Therefore, the final morphology appeared after an additional several weeks. Muscle activity seemed to facilitate growth of the tuber calcanei, but growth of the other parts of calcaneus, including the sustentaculum, seemed to depend on active proliferation at the different sites of cartilage. Multiple tendons and ligaments seemed to fix the talus so that it remained close to the calcaneus.
Virtues as protective factors for adolescent mental health
Authors: McLoughlin Shane , Kristjánsson Kristján 
Published: July 2024

AbstractThis paper explores the decline in adolescent mental health and the weakening of traditional moral frameworks, positing education in the virtues as protective of mental health due to the intrinsic link between moral/existential wellbeing and psychological health. By integrating character education into school curricula, a continuous “dosage” of moral guidance may be an optimal way to ensure a gradual and ever‐clearer articulation of a life worth living and how to live well. The paper critiques popular clinical and positive psychological approaches to promoting wellbeing, which often miss the existential and moral dimensions of adolescent growth. The conclusion emphasizes the need for integrating moral education into mental health interventions to address the comprehensive existential and moral dimensions of adolescent development. This paper advocates for a proactive character developmental model that nurtures moral and existential growth, recognizing challenges with virtue and meta‐virtue development as integral to personal and moral evolution, and enhancing the moral and psychological fortitude of adolescents.
In‐vitro and in‐vivo assessment of biological control potential of nematode symbiont Xenorhabdus nematophila against Pseudomonas syringae, the causative agent of angular leaf spot of cucumber
Authors: Gadhi Muswar Ali , Nazir Talha , Majeed Muhammad Zeeshan , Jatoi Ghulam Hussain , Jie Ren , Qiu Dewen 
Published: July 2024

AbstractBacterial diseases cause colossal losses in vegetable crops and are considered major limiting factors for the commercial production of vegetables around the globe. Among bacterial diseases, angular leaf spot of cucumber caused by Pseudomonas syringae is one of the most economically important diseases that cause substantial economic loss to the cucumber crop. In this study, the in‐vitro and in‐vivo effects of different strains (X1–X15) of the nematode symbiotic bacterium Xenorhabdus nematophila was determined on the growth of P. syringae in different selected media (TB, SB, SOB, SOC, YT and LB) and at different pH levels (6.5, 7.5 and 8.5) and on the incidence of angular leaf spot disease on potted cucumber plants. According to the results, maximum growth inhibition of P. syringe (25 mm) was observed in the case of strain X12, followed by strains X10 (23 mm), X8 (21 mm) and X6 (20 mm), while maximum colony growth was observed in the control. Moreover, this P. syringae growth inhibition by the X12 strain of X. nematophila was significantly maximum in SB medium and at pH 7.5. Results of the in‐vivo experiment with potted plants showed a maximum incidence (90%) of angular leaf spot disease in control plants, while a minimum disease incidence (15%) was observed for the plants treated with two times dilution of X. nematophila culture, followed by ten times (23%) and fifty times (35%). The overall results of this study demonstrate the inhibitory effect of X. nematophila on the growth and incidence of P. syringae and underscore its putative role in the biological management of plant pathogenic bacteria.
Characterization of pectolytic bacteria causing soft rot on sugar beet in the west of Iran
Authors: Seif Shamimeh , Moarrefzadeh Nahid , Sharifi Rouhallah , Hosseini Saman 
Published: July 2024

AbstractBacterial soft rots are amongst the most economically important diseases that cause significant yield losses in various crops. A clear identification of the causal agents is required to develop management strategies. In 2022 and 2023, after the widespread occurrence of soft rot disease in sugar beet fields in the Firuzan region, Hamedan Province, Iran, the current study was conducted to identify and characterize the causal agents. A total of 38 bacterial isolates were obtained from sugar beet plants with soft rot symptoms and subjected to biochemical and phenotypical characterization and pathogenicity tests on healthy slices from potato tubers and sugar beet taproots. Twelve isolates from this study along with the type strain Pectobacterium carotovorum ICMP 5702 were selected for subsequent screening by BOX‐PCR. Six isolates were chosen for sequencing the 16SrRNA gene and phylogenetic analysis. In BLAST analyses, the pathogenic isolates S2, S9, S22, S11, S23 and 111 showed the highest similarity values (≥99%) with the reference sequences of Raoultella terrigena, Klebsiella michiganensis, Erwinia (Winslowiella) iniecta, Pantoea agglomerans and P. carotovorum, respectively. These isolates induced soft rot and maceration on plant materials from five species (bell pepper fruits, garlic cloves and slices from sugar beet taproot, potato tubers and carrot roots) 72 h after inoculation. In the greenhouse experiment, they caused soft rot symptoms in the taproots of 2‐month‐old sugar beet plants 3 weeks after inoculation. These soft rot agents had Gram‐negative, catalase‐positive and oxidase‐negative reactions, grew under both aerobic and anaerobic conditions, tolerated and grew at 37°C and 5% NaCl. In general, the results of this research showed that in addition to Pectobacterium, four other genera from the order Enterobacterales including Erwinia, Pantoea, Klebsiella and Raoultella, are amongst the causes of soft rot in sugar beet in Firuzan region of Hamedan.
A case‐control study to investigate determinants of undernutrition in community‐dwelling children
Authors: Mardani Raden Ahmad Dedy , Hajri Zuhratul , Thoyibah Zurriyatun , Yolanda Henny , Kuo Shu‐Yu , Shyu Yuh‐Kae , Huang Hui‐Chuan 
Published: July 2024

AbstractPurposeThis study aimed to examine determinants of undernutrition among children under 2 years of age.Design and MethodsA matched case‐control study design was conducted to recruit 300 mothers comprising 100 mothers with an undernourished child (case group) and 200 mothers with a healthy child (control group). Measurements consisted of demographic characteristics of children data, mother's data, household data, mother's knowledge of child undernutrition, mother's knowledge of nutrition, complementary feeding practices, and undernutrition parameters of the children. A conditional logistic regression was used to identify determinants of undernutrition. The risk of undernutrition was estimated by odds ratios (ORs) and 95% confidence intervals (CIs). Statistical significance was defined as any p value of <.05.ResultsFindings showed that mother's knowledge of undernutrition (AOR: 0.95; 95% CI: 0.91−0.98), mother's knowledge of nutrition (AOR: 0.90; 95% CI: 0.85−0.96), and mother's knowledge (AOR: 0.78; 95% CI: 0.67−0.91) and behavior of complementary feeding practices (AOR: 0.97; 95% CI: 0.94−0.99) were significant determinants of undernutrition (p < .05).Practice ImplicationsMothers with high scores on knowledge of undernutrition, knowledge of nutrition, and knowledge and behaviors of complementary feeding practices would benefit the children under 2 years to reduce the risk of undernutrition. Healthcare professionals (i.e., pediatric nurses and community health nurses) should provide early assessment of knowledge related to undernutrition, nutrition, and complementary feeding practices for mothers with children under 2 years.
Ageing‐related modification of sleep and breathing in orexin‐knockout narcoleptic mice
Authors: Bastianini Stefano , Alvente Sara , Berteotti Chiara , Lo Martire Viviana , Matteoli Gabriele , Miglioranza Elena , Silvani Alessandro , Zoccoli Giovanna 
Published: July 2024

SummaryNarcolepsy type‐1 (NT1) is a lifelong sleep disease, characterised by impairment of the orexinergic system, with a typical onset during adolescence and young adulthood. Since the wake–sleep cycle physiologically changes with ageing, this study aims to compare sleep patterns between orexin‐knockout (KO) and wild type (WT) control mice at different ages. Four groups of age‐matched female KO and WT mice (16 weeks of age: 8 KO‐YO and 9 WT‐YO mice; 87 weeks of age: 13 KO‐OLD and 12 WT‐OLD mice) were implanted with electrodes for discriminating wakefulness, rapid‐eye‐movement sleep (REMS), and non‐REMS (NREMS). Mice were recorded for 48 h in their home cages and for 7 more hours into a plethysmographic chamber to characterise their sleep‐breathing pattern. Regardless of orexin deficiency, OLD mice spent less time awake and had fragmentation of this behavioural state showing more bouts of shorter length than YO mice. OLD mice also had more NREMS bouts and less frequent NREMS apneas than YO mice. Regardless of age, KO mice showed cataplexy‐like episodes and shorter REMS latency than WT controls and had a faster breathing rate and an increased minute ventilation during REMS. KO mice also had more wakefulness, NREMS and REMS bouts, and a shorter mean length of wakefulness bouts than WT controls. Our experiment indicated that the lack of orexins as well as ageing importantly modulate the sleep and breathing phenotype in mice. The narcoleptic phenotype caused by orexin deficiency in female mice was substantially preserved with ageing.
The chromosome‐level genome assembly of Cananga odorata provides insights into its evolution and terpenoid biosynthesis
Authors: Zheng Yan , Yang Danni , Yin Xin , Yang Xingyu , Chen Mingyue , Li Xieshengyang , Yang Tianyu , Strijk Joeri Sergej , Hinsinger Damien Daniel , Yang Yunqiang , Kong Xiangxiang , Yang Yongping 
Published: July 2024

Summary

Cananga odorata is known as a natural perfume tree of the Annonaceae family in Magnoliales. However, its phylogenetic position and the molecular mechanisms involved in the biosynthesis of the floral volatile organic compounds (VOCs) remain unclear.

Here, by combining a variety of sequencing platforms, we present a telomere‐to‐telomere (T2T) genome of C. odorata with 735.83 Mb, which represents the highest integrity and assembly quality of genome in magnoliid plants reported to date. Phylogenetic analysis based on multiple datasets and approaches showed that C. odorata, as a member of magnoliids, is sister to eudicots, after their divergence from monocots.

Metabolomic of VOCs in the essential oil and flowers scent showed that sesquiterpenes, especially β‐caryophyllene, were the major compounds. Two CoTPS21 homologues derived from tandem duplication events were highly expressed during flower development and were identified as the key sesquiterpene synthases for the production of β‐caryophyllene. In addition, CoSPL3 and CoSPL9 were considered as potential transcription factors for activating the expression of CoTPS21 homologues.

Our results shed light on the molecular mechanisms underlying the biosynthesis of the unique floral fragrance in C. odorata and provide new insights into the phylogenetic position of magnoliids.
Linking music, place and heritage: Anthonie Tonnon's Rail Land
Authors: Kearns Robin 
Published: July 2024

AbstractI investigate intersections between music and mobilities as manifested in songwriter and musician Anthonie Tonnon's Rail Land tour of 2019–20. These concerts celebrated New Zealand's passenger rail heritage through not only performance but also by mobilising the audience to travel by train to the concert venue. The curation of both journey and destination amounted to a subtle activism. In influencing the way people travelled to his shows, Tonnon effectively remobilised a consideration of train travel in which the journey was arguably as memorable as the destination. As such, the artist was contributing to a re‐entwining of music, identity and place.
Influence of red–blue light ratio on the phenology and morphology of different lentil (Lens culinaris Medik) and chickpea (Cicer arietinum) genotypes under a simple and resource‐efficient in‐house speed breeding method based on the application of extended photoperiod
Authors: Mitache Mohammed , Zeroual Abdelmonim , Baidani Aziz , Bencharki Bouchaib , Idrissi Omar 
Published: July 2024

AbstractIn the complex context of rising food demand, natural resources degradation and worsening climate changes impact, food legumes could play an important role in sustainable agriculture and food security. To overcome these challenges, enhanced genetic gain has become a necessity. Speed breeding‐based extended photoperiod is being used to reinforce conventional breeding methods and to speed up the development of adapted varieties. Speed breeding growth chambers and greenhouses' light quality in terms of red–blue ratio is among important factors that need to be optimized to enhance the efficiency of this technique to accelerate plant growth and development while limiting plant stress. We have compared the effect of different light ratios of 5:3 red–blue, 3:5 red–blue and 4:4 white–white on phenological and morphological characteristics of three lentil and chickpea genotypes grown in a speed breeding‐based extended photoperiod growth chamber with 22 h light/2 h dark. The 5:3 red–blue light ratio increased plant height and reduced flowering time for the three tested lentil varieties with 36, 41 and 40 days after sowing, respectively, as well as for the three tested chickpea varieties with 42, 38 and 24 days after sowing, respectively. While the 3:5 red–blue ratio increased leaves and secondary stem growth for lentil; leaves and secondary stem growth, green canopy cover and seedling vigour for chickpea. Less vegetative growth and later flowering were observed under white light. Higher red–blue light ratio of 5:3 accelerated lentil and chickpea vegetative growth and flowering, making it more optimal for speed breeding‐based extended photoperiod. It should be pointed out that the suggested home‐designed and built protocol is cost‐effective and easy to implement especially in small‐scale breeding programmes in developing countries.
Norms of Reasoning
Authors: McHugh Conor 
Published: July 2024

AbstractWhen we reason, we can be assessed against diverse norms. Unfortunately different types of such norms are often conflated. This article distinguishes some different types of norms to which we are subject when we reason, and shows how this can help to clarify certain philosophical debates. It then considers, briefly, ‘norms of starting points’, and, at more length, ‘norms of transitions’. In closing it briefly considers whether we might expect to find a unifying account of the source of these norms, and if so what it might look like.
The deliberative constraint on reasons
Authors: Schultz Conner 
Published: July 2024

AbstractMust reasons be able to feature in our deliberation? Proponents of a deliberative constraint on reasons endorse an affirmative answer to this question. Deliberative constraints enjoy broad appeal and have been deployed as premises in support of a variety of controversial philosophical positions. Yet, despite their uses, deliberative constraints have not received systematic philosophical attention. This entry aims to fill this gap in the literature. First, I sketch what's at stake in the debate over whether a deliberative constraint is true. Then, I offer a taxonomy of the different versions of a deliberative constraint. Finally, I assess some of the arguments for and against a deliberative constraint.
The effect of different parameters of low‐level laser used in the treatment of oral mucositis, on the viability and apoptosis of oral squamous cell carcinoma cells: In vitro study
Authors: Shirazian Shiva , Mohseni Atieh , Pourshahidi Sara , Alaeddini Mojgan , Etemad‐Moghadam Shahroo , Vatanpour Mehdi 
Published: July 2024

AbstractOral mucositis is a complication of chemo/radiotherapy. To assess the impact of various power levels of diode‐laser on the survival and expression of apoptosis‐related genes in oral cancer cells, it is crucial to consider the potential existence of malignant cells within the treatment region and the reliance of laser effectiveness on its specific characteristics. Cal‐27 cells were cultivated and exposed to a 660 nm‐diode‐laser at power levels of 20, 40, and 80 mW, alongside non‐irradiated control cells. Viability and expression of Bax and Bcl‐2 mRNA were assessed with Methyl Thiazolyl Tetrazolium (MTT) and Real‐time Polymerase Chain Reaction (RT‐PCR), respectively. The results were analyzed using one‐way ANOVA and Tukey post‐hoc test (p < 0.05). A significant reduction in viability was found only in the 20 mW group compared to controls (p = 0.001). Cell survival was significantly lower in cells receiving 20 mW laser than those treated with 40 and 80 mW (p < 0.05). None of the laser groups showed significant changes in BcL‐2, but Bax was significantly lower in cells receiving 40 and 80 mW (p < 0.05), compared to controls. Laser irradiation at 660 nm (2 J/cm2, 30 s) significantly reduced the viability of oral cancer cells when using 20 mW power. These specifications align with the recommendation that the lowest possible laser dose should be applied for treating cancer patients. The exact mechanism of cell death following laser therapy with these specifications requires further investigation.
Tracking heterogenous protein aggregation at nanoscale through fluorescence correlation spectroscopy
Authors: Halder Bisal , Ghosh Shreya , Khan Tanmoy , Pal Subhendu , Das Nilimesh , Sen Pratik 
Published: July 2024

AbstractVarious biophysical techniques have been extensively employed to study protein aggregation due to its significance. Traditionally, these methods detect aggregation at micrometer length scales and micromolar concentrations. However, unlike in vitro, protein aggregation typically occurs at nanomolar concentrations in vivo. Here, using fluorescence correlation spectroscopy (FCS), we captured bromelain aggregation at concentrations as low as ~20 nM, surpassing the detection limit of traditional methods like thioflavin T fluorescence, scattering, and fluorescence microscopy by more than one order of magnitude. Moreover, using thioflavin T fluorescence‐based FCS, we have detected larger aggregates at higher bromelain concentrations, which is undetectable in FCS otherwise. Importantly, our study reveals inherent heterogeneity in bromelain aggregation, inaccessible to ensemble‐averaged techniques. The presented report may provide a platform for the characterization of premature aggregates at very low protein concentrations, which are thought to be functionally significant species in protein aggregation‐induced diseases.
Far‐UVC: The impact of optical filters on real‐world deployment
Authors: Eadie Ewan , O'Mahoney Paul , Ibbotson Sally H. , Miller C. Cameron , Wood Kenneth 
Published: July 2024

AbstractIn 2015, a study showed that Krypton‐Chloride (KrCl) excimer lamps could induce erythema and basal layer DNA damage in human skin. Later studies found that filtering out longer wavelength emissions from these lamps resulted in no acute skin effects. However, there is a limited understanding of how much to reduce unwanted emissions and which wavelengths are important. Accurate spectral irradiance data is therefore crucial for safety, as variance in optical filtering significantly affects the weighted irradiance of a lamp. To simplify the risk assessment process for Far‐UVC lamps, we highlight the usefulness of the lamp exposure limit (HLEL) and present this in the context of spectral emission data for 14 commercially available Far‐UVC lamps. Our results demonstrate that relying solely on a radiometric measurement and a single‐wavelength exposure limit at 222 nm could lead to over‐exposure. The HLEL is a practical metric which can be utilized to determine the exposure time before reaching the exposure limit. It can also be used in the determination of the minimum ceiling height for compliance with standards like UL 8802. Manufacturers are urged to provide HLEL for their products; installers should adhere to HLEL; and standards and regulatory bodies should insist on this information in new guidance.
How lignin biosynthesis responds to nitrogen in plants: a scoping review
Authors: Peng Q. , Shrestha A. , Zhang Y. , Fan J. , Yu F. , Wang G. 
Published: July 2024

AbstractNitrogen (N) plays a critical role in the functioning of key amino acids and synthetic enzymes responsible for the various stages of lignin biosynthesis. However, the precise mechanisms through which N influences lignin biosynthesis have not been fully elucidated. This scoping review explores how lignin biosynthesis responds to N in plants. A systematic search of the literature in several databases was conducted using relevant keywords. Only 44 of the 1842 selected studies contained a range of plant species, experimental conditions, and research approaches. Lignin content, structure, and biosynthetic pathways in response to N are discussed, and possible response mechanisms of lignin under low N are proposed. Among the selected studies, 64.52% of the studies reter to lignin content found a negative correlation between N availability and lignin content. Usually, high N decreases the lignin content, delays cell lignification, increases p‐hydroxyphenyl propane (H) monomer content, and regulates lignin synthesis through the expression of key genes (PAL, 4CL, CCR, CAD, COMT, LAC, and POD) encoding miRNAs and transcription factors (e.g., MYB, bHLH). N deficiency enhances lignin synthesis through the accumulation of phenylpropanoids, phenolics, and soluble carbohydrates, and indirect changes in phytohormones, secondary metabolites, etc. This review provides new insights and important references for future studies on the regulation of lignin biosynthesis.