bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comprehensive microRNA expression profile of the backfat tissue from castrated and intact full-sib pair male pigs
Authors: Bai Ying , Huang Jin-Ming , Liu Gang , Zhang Ji-Bin , Wang Jian-Ying , Liu Cheng-Kun , Fang Mei-Ying 
Published: January 2014

AbstractBackgroundIt is widely known that castration has a significant effect on the accumulation of adipose tissue. microRNAs (miRNAs) are known to be involved in fat deposition and to be regulated by the androgen-induced androgen receptor (AR). However, there is little understanding of the relationship between miRNAs and fat deposition after castration. In this study, the high-throughput SOLiD sequencing approach was used to identify and characterize miRNA expression in backfat from intact and castrated full-sib male 23-week-old pigs. The patterns of adipogenesis and fat deposition were compared between castrated and intact male pigs.ResultsA total of 366 unique miRNA genes were identified, comprising 174 known pre-miRNAs and 192 novel pre-miRNAs. One hundred and sixty-seven pre-miRNAs were common to both castrated (F3) and intact (F4) male pig small RNA libraries. The novel pre-miRNAs encoded 153 miRNAs/miRNA*s and 141 miRNAs/miRNA*s in the F3 and F4 libraries, respectively. One hundred and seventy-seven miRNAs, including 45 up- and 132 down-regulated, had more than 2-fold differential expression between the castrated and intact male pigs (p-value < 0.001). Thirty-five miRNAs were further selected, based on the expression abundance and differentiation between the two libraries, to predict their targets in KEGG pathways. KEGG pathway analyses suggested that miRNAs differentially expressed between the castrated and intact male pigs are involved in proliferation, apoptosis, differentiation, migration, adipose tissue development and other important biological processes. The expression patterns of eight arbitrarily selected miRNAs were validated by stem-loop reverse-transcription quantitative polymerase chain reaction. These data confirmed the expression tendency observed with SOLiD sequencing. miRNA isomiRs and mirtrons were also investigated in this study. Mirtrons are a recently described category of miRNA relying on splicing rather than processing by the microprocessor complex to generate the RNAi pathway. The functions of miRNAs important for regulating fat deposition were also investigated in this study.ConclusionsThis study expands the number of fat-deposition-related miRNAs in pig. The results also indicate that castration can significantly affect the expression patterns of fat-related miRNAs. The differentially expressed miRNAs may play important roles in fat deposition after castration.
Identification of mechanosensitive genes during skeletal development: alteration of genes associated with cytoskeletal rearrangement and cell signalling pathways
Authors: Rolfe Rebecca A , Nowlan Niamh C , Kenny Elaine M , Cormican Paul , Morris Derek W , Prendergast Patrick J , Kelly Daniel , Murphy Paula 
Published: January 2014

AbstractBackgroundMechanical stimulation is necessary for regulating correct formation of the skeleton. Here we test the hypothesis that mechanical stimulation of the embryonic skeletal system impacts expression levels of genes implicated in developmentally important signalling pathways in a genome wide approach. We use a mutant mouse model with altered mechanical stimulation due to the absence of limb skeletal muscle (Splotch-delayed) where muscle-less embryos show specific defects in skeletal elements including delayed ossification, changes in the size and shape of cartilage rudiments and joint fusion. We used Microarray and RNA sequencing analysis tools to identify differentially expressed genes between muscle-less and control embryonic (TS23) humerus tissue.ResultsWe found that 680 independent genes were down-regulated and 452 genes up-regulated in humeri from muscle-less Spd embryos compared to littermate controls (at least 2-fold; corrected p-value ≤0.05). We analysed the resulting differentially expressed gene sets using Gene Ontology annotations to identify significant enrichment of genes associated with particular biological processes, showing that removal of mechanical stimuli from muscle contractions affected genes associated with development and differentiation, cytoskeletal architecture and cell signalling. Among cell signalling pathways, the most strongly disturbed was Wnt signalling, with 34 genes including 19 pathway target genes affected. Spatial gene expression analysis showed that both a Wnt ligand encoding gene (Wnt4) and a pathway antagonist (Sfrp2) are up-regulated specifically in the developing joint line, while the expression of a Wnt target gene,Cd44, is no longer detectable in muscle-less embryos. The identification of 84 genes associated with the cytoskeleton that are down-regulated in the absence of muscle indicates a number of candidate genes that are both mechanoresponsive and potentially involved in mechanotransduction, converting a mechanical stimulus into a transcriptional response.ConclusionsThis work identifies key developmental regulatory genes impacted by altered mechanical stimulation, sheds light on the molecular mechanisms that interpret mechanical stimulation during skeletal development and provides valuable resources for further investigation of the mechanistic basis of mechanoregulation. In particular it highlights the Wnt signalling pathway as a potential point of integration of mechanical and molecular signalling and cytoskeletal components as mediators of the response.
Hospital-based HIV/HSV-2 seroprevalence among male patients with anal disease in Korea: cross sectional study
Authors: Wang Jin-Sook , Hwang Do Yeon , Yu Hye-Kyung , Kim Sung Soon , Lee Jong Kyun , Kee Mee-Kyung 
Published: January 2014

Abstract

Background
This study aimed to identify the characteristics of HIV and herpes simplex virus (HSV)-2 seroprevalence in male patients with anal disease.

Methods
HIV seroprevalence was estimated for different age groups of male patients with anal disease who were treated at Songdo colorectal hospital in Korea between 2001 and 2011. HIV seroprevalence of patients with anal disease was compared with that of patients with nonanal disease for each year from 2007 to 2011. HSV-2 antibody tests were conducted on 2,038 HIV-tested male patients with anal disease in 2009.

Results
For 11 years from 2001, HIV seroprevalence differed significantly by age group (P < 0.001) and was highest in the group aged <20 years. From 2007 to 2011, HIV seroprevalence in patients with anal disease was 7.6/10,000–13.3/10,000 and that in patients with nonanal disease was 0–0.9/10,000. HSV-2 seroprevalence among patients with anal disease was 24.0%, and only one patient with HIV and HSV-2 was observed.

Conclusions
HIV seroprevalencein male patients with anal disease was significantly higher than that for other diseases. HSV-2 seroprevalence was similar to that in the general male population. Implementation of the current HIV surveillance system for male patients at colorectal hospitals is necessary to help prevent HIV transmission.
Host biomarkers distinguish dengue from leptospirosis in Colombia: a case–control study
Authors: Conroy Andrea L , Gélvez Margarita , Hawkes Michael , Rajwans Nimerta , Liles W Conrad , Villar-Centeno Luis Angel , Kain Kevin C 
Published: January 2014

AbstractBackgroundDengue fever and leptospirosis have partially overlapping geographic distributions, similar clinical presentations and potentially life-threatening complications but require different treatments. Distinguishing between these cosmopolitan emerging pathogens represents a diagnostic dilemma of global importance. We hypothesized that perturbations in host biomarkers can differentiate between individuals with dengue fever and leptospirosis during the acute phase of illness.MethodsWe randomly selected subjects from a prospective cohort study of acute febrile illness in Bucaramanga, Colombia and tested 19 serum biomarkers by ELISA in dengue fever (DF, n = 113) compared to subjects with leptospirosis (n = 47). Biomarkers were selected for further analysis if they had good discriminatory ability (area under the ROC curve (AUC) >0.80) and were beyond a reference range (assessed using local healthy controls).ResultsNine biomarkers differed significantly between dengue fever and leptospirosis, with higher levels of Angptl3, IL-18BP, IP-10/CXCL10, Platelet Factor 4, sICAM-1, Factor D, sEng and sKDR in dengue and higher levels of sTie-2 in leptospirosis (p < 0.001 for all comparisons). Two biomarkers, sEng and IL18BP, showed excellent discriminatory ability (AUROC >0.90). When incorporated into multivariable models, sEng and IL18BP improved the diagnostic accuracy of clinical information alone.ConclusionsThese results suggest that host biomarkers may have utility in differentiating between dengue and leptospirosis, clinically similar conditions of different etiology.
High prevalence of Y-box protein-1/p18 fragment in plasma of patients with malignancies of different origin
Authors: Tacke Frank , Galm Oliver , Kanig Nicolas , Yagmur Eray , Brandt Sabine , Lindquist Jonathan A , Eberhardt Christiane S , Raffetseder Ute , Mertens Peter R 
Published: January 2014

Abstract

Background
Expression of the cold shock protein Y-box protein 1 (YB-1) is associated with deleterious outcome in various malignant diseases. Our group recently showed that the detection of an 18 kDa YB-1 fragment (YB-1/p18) in human plasma identifies patients with malignant diseases. We now tested the prevalence, clinical, and diagnostic value of YB-1/p18 detection in common tumors.

Methods
A newly established monoclonal YB-1 antibody was used to detect YB-1/p18 by immunoblotting in plasma samples from 151 unselected tumor patients, alongside established tumor markers and various diagnostic measures, during evaluation for a cancerous disease and in follow-up studies after therapeutic interventions.

Results
Circulating YB-1/p18 was detected in 78% of patients having a tumor disease. YB-1/p18 positivity was highly prevalent in all examined malignancies, including lung cancer (32/37; 87%), breast cancer (7/10; 70%), cancer of unknown primary (CUP; 5/5, 100%) or hematological malignancies (42/62; 68%). Positivity for YB-1/p18 was independent of other routine laboratory parameters, tumor stage, or histology. In comparison to 13 established tumor markers (cancer antigens 15–3, 19–9, 72–4, and 125; carcinoembryonic antigen; cytokeratin fragments 21–1; neuron-specific enolase; alpha-fetoprotein; beta-2-microglobulin; squamous cell carcinoma antigen; thymidine kinase; tissue polypeptide antigen; pro-gastrin-releasing peptide), YB-1/p18 detection within serum samples was the most sensitive general parameter identifying malignant disorders. YB-1/p18 concentrations altered during therapeutic interventions, but did not predict prognosis.

Conclusions
Plasma YB-1/p18 detection has a high specific prevalence in malignancies, thereby providing a novel tool for cancer screening independent of the tumor origin.
Descemet membrane endothelial keratoplasty in cases with existing scleral-sutured and iris-sutured intraocular lenses
Authors: Röck Daniel , Röck Tobias , Bartz-Schmidt Karl-Ulrich , Yoeruek Efdal 
Published: January 2014

Abstract

Background
To report two cases of Descemet Membrane Endothelial Keratoplasty (DMEK) in patients with existing scleral-fixated and iris-fixated intraocular lenses (sf-IOL and if-IOL, respectively).

Case presentation
DMEK procedures were performed on a 49-year-old woman with a pre-existing sf-IOL (case 1) and a 69-year-old woman with a pre-existing if-IOL (case 2) in order to treat secondary corneal edema due to pseudophakic bullous keratopathy. Visual acuity, refractive error, intraocular pressure, slit lamp examination, pachymetry measurements and endothelial cell density (ECD) were considered and repeated during follow-ups.
Both cases had no intraoperative complications. At postoperative day 1 graft centration and complete attachment were noted. The IOL positions were unchanged in comparison to their preoperative positions. In case 1, visual acuity improved from 1/15 at 1 meter preoperative to 20/200 within one week and to 20/63 within 12 weeks of follow up. In case 2, visual acuity improved from counting fingers at 1 meter preoperative to 20/200 within one week and to 20/100 within 12 weeks of follow-up. In case 2 a partial graft dislocation was observed at postoperative day twenty. Complete graft re-apposition was achieved by rebubbling procedure performed with intracameral air injection.

Conclusions
DMEK surgery in the treatment of pseudophakic bullous keratopathy in the presence of sf-IOL and if-IOL can successfully be performed. These eyes are at increased risk of IOL dislocation into the vitreous cavity during DMEK surgery.
Response of an aggressive periosteal aneurysmal bone cyst (ABC) of the radius to denosumab therapy
Authors: Pauli Chantal , Fuchs Bruno , Pfirrmann Christian , Bridge Julia A , Hofer Silvia , Bode Beata 
Published: January 2014

AbstractAneurysmal bone cyst (ABC), once considered a reactive lesion, has been proven to be a neoplasia characterized by rearrangements of theUSP6-gene. Aggressive local growth and recurrences are common and therapeutic options may be limited due to the vicinity of crucial structures. We describe a case of a locally aggressive, multinucleated giant cell-containing lesion of the forearm of a 21-year old woman, treated with denosumab for recurrent, surgically uncontrollable disease. Under the influence of this RANKL inhibitor, the tumor showed a marked reduction of the content of the osteoclastic giant cells and an extensive metaplastic osteoid production leading to the bony containment, mostly located intracortically in the proximal radius. The diagnosis of a periosteal ABC was confirmed by FISH demonstratingUSP6gene rearrangement on the initial biopsy. Function conserving surgery could be performed, enabling reconstruction of the affected bone. Inhibition of RANKL with denosumab may offer therapeutic option for patients not only with giant cell tumors but also with ABCs.
Fabrication of micro-ridge long-period gratings inscribed on polarization-maintaining fibers
Authors: Kwon Oh-Jang , Shin Myungjun , Han Young-Guen 
Published: January 2014

Abstract
We experimentally investigated a simple and new technique for the fabrication of micro-ridge long-period gratings (MRLPGs) based on polarization-maintaining fibers (PMFs). The cladding region of the PMFs was etched periodically using a wet etching technique resulting in the periodic formation of micro-ridges on the surface of the PMF. The PMF-based MRLPGs has two resonant peaks because of the birefringence of the PMF. The extinction ratios of two resonant peaks of the PMF-based MRLPGs were effectively improved by increasing the applied strain because of the photoelastic effect.
New results on the Baire partial quasi-metric space, fixed point theory and asymptoticcomplexity analysis for recursive programs
Authors: Alghamdi Maryam A , Shahzad Naseer , Valero Oscar 
Published: January 2014

Abstract
In Cerdà-Uguet et al. (Theory Comput. Syst. 50:387-399, 2012), a newmathematical fixed point technique, that uses the so-called Baire partial quasi-metricspace, was introduced with the aim of providing the asymptotic complexity of a class ofrecursive algorithms. The aforementioned technique presents the advantage that requiresless calculations than the quasi-metric original one given by Schellekens (Electron. NotesTheor. Comput. Sci. 1:211-232, 1995). In this paper we continue the study, started inCerdà-Uguet et al. (Theory Comput. Syst. 50:387-399, 2012), on the use ofpartial quasi-metric spaces for asymptotic complexity analysis of algorithms. Concretely,our main purpose is to prove that the Baire partial quasi-metric space is an appropriatemathematical framework for discussing via fixed point arguments the asymptotic complexityof a general class of recursive algorithms to which all the algorithms analyzed inCerdà-Uguet et al. (Theory Comput. Syst. 50:387-399, 2012) belong. Theobtained results are illustrated by means of applying them to yield the complexity of twocelebrated recursive algorithms which don not belong to the class discussed inCerdà-Uguet et al. (Theory Comput. Syst. 50:387-399, 2012).

MSC: 47H10, 54E50, 68Q15, 68Q25, 68W40.
A new type fixed point theorem for a contraction on partially ordered generalized complete metric spaces with applications
Authors: Eshaghi Gordji Madjid , Ramezani Maryam , Sajadian Farhad , Cho Yeol Je , Park Choonkil 
Published: January 2014

Abstract
In this paper, we prove a fixed point theorem for a contraction in generalized complete metric spaces endowed with partial order. As an application, we use the fixed point theorem to prove the Hyers-Ulam stability of the Cauchy functional equation in Banach spaces endowed with a partial order.

MSC:54H25, 47H10, 39B52.
Existence of solutions for fractional differential equations with integral boundary conditions
Authors: Yan Rian , Sun Shurong , Lu Hongling , Zhao Yan 
Published: January 2014

Abstract
In this paper, we study boundary-value problems for the following nonlinear fractional differential equations involving the Caputo fractional derivative:

D

0
+

α

C

x
(
t
)
=
f
(
t
,
x
(
t
)

,
C

D

0
+

β

x
(
t
)
)

,

t
∈
[
0
,
1
]

,

x
(
0
)
+

x
′

(
0
)
=
y
(
x
)

,

∫
0
1

x
(
t
)

d
t
=
m

,

x
″

(
0
)
=

x
‴

(
0
)
=
⋯
=

x

(
n
−
1
)

(
0
)
=
0

, where

D

0
+

α

C

,

D

0
+

β

C

are the Caputo fractional derivatives,

f
:
[
0
,
1
]
×
R
×
R
→
R

is a continuous function,

y
:
C
(
[
0
,
1
]
,
R
)
→
R

is a continuous function and

m
∈
R

,

n
−
1
<
α
<
n

(

n
≥
2

),

0
<
β
<
1

is a real number. By means of the Banach fixed-point theorem and the Schauder fixed-point theorem, some solutions are obtained, respectively. As applications, some examples are presented to illustrate our main results.

MSC:34A08, 34B10.
Association of XPD Lys751Gln polymorphism with head and neck cancer susceptibility: evidence from 11,443 subjects
Authors: Lin Hai , Lin Dong , Zheng Chunquan 
Published: January 2014

Abstract

Background
Whether the single nucleotide polymorphism (SNP) Lys751Gln of xeroderma pigmentosum group D(XPD) gene increases susceptibility to head and neck cancer (HNC) is controversial and undetermined. Therefore, we conducted this meta-analysis to systematically assess the possible association between them.

Methods
The OVID, Medline, Embase, Pubmed, Web of Science databases were searched to identify the eligible studies. The odds ratio (OR) with 95% confidence interval (95% CI) were used to assess the strength of association.

Results
A total of 11,443 subjects from eighteen studies were subjected to meta-analysis. Overall, XPD Lys751Gln polymorphism had no association with increased HNC risk under all five genetic models (P > 0.05). In the subgroup analysis by ethnicity and source of controls, still no significant association was found under five genetic models (P > 0.05). In the subgroup analysis by cancer type, XPD Lys751Gln polymorphism had statistically significant association with elevated laryngeal cancer (LC) and nasopharyngeal cancer (NPC) risk under heterozygous comparison and dominant model (P<0.05) and borderline significantly increased risk was found under allele contrast for LC and NPC. Carriers of Lys allele and Lys/Lys genotype may be associated with elevated LC and NPC risk.

Conclusions
There is overall lack of association between XPD Lys751Gln polymorphism and HNC risk under all five genetic models and still no significant association was found in the subgroup analysis by ethnicity and source of controls. However, XPD Lys751Gln polymorphism was significantly associated with susceptibility to LC and NPC and the Lys allele and Lys/Lys genotype of XPD Lys751Gln polymorphism may be a risk factor for LC and NPC. However, relatively modest sample sizes were included in this meta-analysis and studies with large sample sizes and representative population are warranted to further clarify this finding.

Virtual slides
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/5628716106316015.
Effects of foliar application of salicylic acid and nitric oxide in alleviating iron deficiency induced chlorosis of Arachis hypogaea L.
Authors: Kong Jing , Dong Yuanjie , Xu Linlin , Liu Shuang , Bai Xiaoying 
Published: January 2014

Abstract

Background
The aim of this experiment was to analyze the alleviation mechanism of exogenous salicylic acid (SA) and sodium nitroprusside (SNP, a nitric oxide donor) on peanut seedlings under Fe deficiency. The effects of SA and SNP on iron uptake and availability, ions balance and oxidant damage were studied with foliar application of exogenous 1.0 mM SA (SA) or 2.5 mM SNP (SNP) or 0.5 mM SA+1.25 mM SNP [1/2(SA+SNP)] or 1.0 mM SA+2.5 mM SNP (SA+SNP).

Results
The results showed that after 21 days treatment, the peanut seedlings growing under iron deficiency conditions exhibited leaf interveinal chlorosis, and this iron-deficiency induced symptom was prevented by foliar application of SA, SNP, 1/2 (SA+SNP), especially SA+SNP. The increased contents of chlorophyll and active iron, and increased Fe accumulation in cell organelles were observed in SA+SNP treated young leaves, suggesting that an improvement of iron availability in plants. Moreover, the improved nutrient solution pH, increased H+-ATPase activity and increased iron concentration in roots in SA+SNP treated plants, suggesting that SA+SNP is effective in modulating iron uptake. Furthermore, the increased calcium (Ca), magnesium (Mg) and zinc (Zn) concentrations and decreased manganese (Mn) and copper (Cu) concentrations in the leaves and roots of peanut indicated that SA+SNP stimulated the maintenance of ions disturbed by Fe deficiency. In addition, SA+SNP alleviated the increased accumulation of superoxide anion (O2
•-) generation rate and malondialdehyde (MDA), and modulated the antioxidant enzymes.

Conclusions
These results indicated that the interaction of SA and SNP promoted Fe uptake, translocation and activation; modulated the balance of mineral elements; and protected Fe deficiency induced oxidative stress. Therefore, SA and SNP had synergistic effects in alleviating chlorosis induced by Fe deficiency.