bluuebunny/crossref_metadata_2025_split huggingface dataset:
Factors associated with a negative outcome of medication-overuse headache: A 3-year follow-up (the ‘CARE’ protocol)
Authors: Sances Grazia , Galli Federica , Ghiotto Natascia , Allena Marta , Guaschino Elena , Frustaci Alessandra , Nappi Giuseppe , Tassorelli Cristina 
Published: February 2013

Aim To evaluate factors associated with a negative outcome in a 3-year follow-up of subjects diagnosed with medication-overuse headache (MOH) (revised-ICHD-II criteria). Methods All consecutive patients entering the center’s inpatient detoxification program were analyzed in a prospective, non-randomized fashion. All participants were assessed by a neurologist using an ad hoc patient record form. Personality was assessed using the Minnesota Multiphasic Personality Inventory (MMPI)-2, Chi-square test, one-way analysis of variance (ANOVA), and odds ratios (OR) were calculated as appropriate. Results One-hundred and fifty patients completed the follow-up (79.3% females, age 46.40 ± 11.31 years): 13 never stopped their drug overuse (A), 38 stopped their overuse, but relapsed at least once (B), and 99 stopped and never relapsed (C). The Group A patients differed from those in B + C as they were more frequently single (OR 0.134; p = 0.007) and unemployed (OR 3.273; p = 0.04), took a higher number of drug doses ( p < 0.001), and less frequently drank coffee (OR 3.273; p = 0.044). Personality profile: subjects in A scored higher than those in C on the following scales: Hypochondriasis ( p = 0.007), Depression ( p = 0.003), Paranoia ( p = 0.025), Fears ( p = 0.003), Obsessiveness ( p = 0.026), Bizarre Mentation ( p = 0.046), Social Discomfort ( p = 0.004), Negative Treatment Indicators ( p = 0.040), Repression ( p = 0.007), Overcontrolled Hostility ( p = 0.040), Addiction Admission ( p = 0.021), Social Responsibility ( p = 0.039), and Marital Distress ( p = 0.028). Conclusion Disease outcome in MOH patients is influenced negatively by overuse severity and by specific psychological and socio-economic variables. Other possible modifier factors were voluptuary habits.
Novel high-performance nickel/poly(ether ether ketone) nanocomposites
Authors: Kumar Ashish , Mishra TK , Pandey KN , Verma Vishal , Kumar Vijai 
Published: February 2013

The present investigation deals with the preparation and characterization of nanocomposites of poly(ether ether ketone) (PEEK) containing nanosized nickel filler up to 3 wt% loading. Characterization of the developed nanocomposites has been carried out by various advanced analytical techniques. Thermogravimetric analysis study depicts that the prepared nanocomposites exhibit enhanced thermal stability. Dynamic mechanical analysis (DMA) technique has been utilized to investigate the viscoelastic deformation of nanocomposites. From DMA studies, it has been demonstrated that with the increase in nano-nickel content, the stiffness of nanocomposites decreases and a very minute change in glass transition temperature ( Tg) has been noticed. The resulting nanocomposites show the optimum improvement in Young’s modulus, tensile strength, flexural strength and flexural modulus. Scanning electron microscopy study reveals that he dispersion of nano-nickel particulates is uniform throughout the PEEK matrix.
Effect of nano-CaCO3 on the mechanical and crystallization behavior of HDPE/LDPE/nano-CaCO3 ternary blend
Authors: Zaman Haydar U , Khan Mubarak A , Khan Ruhul A , Beg Md. Dalour Hossen 
Published: February 2013

High-density polyethylene–low-density polyethylene (HDPE-LDPE) blends involving HDPE-LDPE (50/50 wt%) with dialkyl peroxide (DAP; 0.05 wt%) containing different amounts (5, 10 and 15 wt%) of nanocalcium carbonate (nCC) were prepared by melt blending followed by compression molding. The effect of addition of nCC on the morphology, mechanical and crystallization behaviors of HDPE-LDPE blends with and without DAP was also evaluated. The measurements of the mechanical properties showed that the impact strength of the nanocomposites increased at first and then decreased with the addition of nanofillers, and the tensile strength was reduced at the same time. With the addition of a small amount of DAP, the mechanical properties of the obtained nanocomposites increased. Scanning electron microscopy observation showed that the addition of DAP improved the dispersion of the nanofiller in the HDPE-LDPE blends. Differential scanning calorimetric results indicated that the addition of DAP increased the crystallization temperature as a result of heterogeneous nucleation effect of nCC on HDPE-LDPE blends.
A 90-day subchronic toxicity study of neem oil, a Azadirachtaindica oil, in mice
Authors: Wang C. , Cao M. , Shi D-X. , Yin Z-Q. , Jia R-Y. , Wang K-Y. , Geng Y. , Wang Y. , Yao X-P. , Yang Z-R. , Zhao J. 
Published: February 2013

To determine the no-observed-adverse-effect level (NOAEL) of exposure and target organs of neem oil for establishing safety criteria for human exposure, the subchronic toxicity study with neem oil in mice was evaluated. The mice (10 per sex for each dose) was orally administered with neem oil with the doses of 0 (to serve as a control), 177, 533 and 1600 mg/kg/day for 90 days. After the treatment period, observation of reversibility or persistence of any toxic effects, mice were continuously fed without treatment for the following 30 days. During the two test periods, the serum biochemistry, organ weight and histopathology were examined. The results showed that the serum biochemistry and organ coefficient in experimental groups had no statistical difference compared with those of the control group. At the 90th day, the histopathological examinations showed that the 1600 mg/kg/day dose of neem oil had varying degrees of damage on each organ except heart, uterus and ovarian. After 30-day recovery, the degree of lesions to the tissues was lessened or even restored. The NOAEL of neem oil was 177 mg/kg/day for mice and the target organs of neem oil were determined to be testicle, liver and kidneys.
The Incidence of Metamizole Sodium-Induced Agranulocytosis in Poland
Authors: Maj S , Lis Y 
Published: February 2013

Metamizole sodium continues to be used extensively as an analgesic in Poland. This study was the first undertaken to quantify the incidence of agranulocytosis associated with metamizole sodium in Poland, and to compare the findings with those from other countries. Based on data obtained retrospectively from medical records covering the period 1997–2001 in six haematology centres representative of Poland as a whole, the crude estimate obtained was a rate of 0.2 cases per million person-days of use. This is the same order of magnitude as the value from an epidemiological study covering several countries (0.3–4.0). W Polsce w dalszym ciągu na dużą skalę stosuje się sól sodową metamizolu jako lek przciwbólowy. Przeprowadzone badanie było pierwsżą próbą ilościowego ustalenia związku pomiędzy wystąpieniem nowych przypadków agranulocytozy a stosowaniem soli sodowej metamizolu w Polsce í porównania rezultatów badania z wynikami uzyskanymi w innych krajach. Na podstawie danych uzyskanych retrospektywnie, z historii chorób obejmujących okres 1997–2001 z sześciu ośrodków hematologicznych będących reprezentacyjnymi dla calej Polski, uzyskana surowa ocena wynosiła 0.2 przypadki na milion osobo/dni stosowania. Otrzymany rząd wielkości mieśći się w dolnym zakresie wyników badań epidemiologicznych w innych krajach (0.3–4.0).
Effects of Glutaraldehyde on Synovial Tissue
Authors: Tosun N , Akpinar F , Dogan A 
Published: February 2013

This study investigated the effect of glutaraldehyde on the synovial tissue of rabbits. Four different concentrations of glutaraldehyde solution were injected into one knee and the contralateral glenohumeral joint of rabbits in group A to group D. The concentration levels used in group A and group B were 1000 ppm and 100 ppm, respectively. For group C and group D, samples of rinsing solutions obtained by rinsing arthroscopic equipment with ***1 l or ***2 l of isotonic saline, respectively, after immersion in 2% glutaraldehyde were used. The contralateral knee and ipsilateral glenohumeral joint of the same rabbits were injected with saline and used as control sites. Rabbits were sacrificed after 1 day, 7 days or 15 days and the histopathological changes in the synovial tissues were compared. The most dramatic inflammatory changes were observed in group A and group B. The histopathological changes were greater after 7 days than after 1 day or 15 days. Group C and group D showed no significant differences when compared with normal synovium. A correlation between the concentration of the glutaraldehyde solution and the number of inflamed joints was observed. Very few or no inflamed joints were seen when samples from rinsing solutions of 1 l or 2 l of saline, respectively, were used.
Identification of the Culprit Artery Involved in Inferior Wall Acute Myocardial Infarction Using Electrocardiographic Criteria
Authors: Bayram E , Atalay C 
Published: February 2013

We tested whether particular electrocardiogram (ECG) changes can identify the right coronary (RCA) or left circumflex (LCX) artery as the responsible vessel in inferior wall acute myocardial infarction (AMI) in 73 patients. A standard 12-lead ECG was performed within 6 h of onset of chest pain. Coronary angiography was performed between 1 week and 6 weeks after the infarction. RCA and LCX lesions were detected in 53 and 20 patients, respectively. The most useful ECG parameters for implicating the RCA were a higher ST elevation in lead III than lead II (specificity 94%, sensitivity 86%) and an S/R wave ratio > 0.33 plus ST segment depression > 1 mm in lead aVL (specificity 94%, sensitivity 92%). Absence of these criteria was associated with LCX occlusion (specificity 100%, sensitivity 87%). These results indicate that composite ECG criteria are useful in predicting the artery involved in inferior wall AMI.
Effects of Trimetazidine on Lipid Peroxidation, Antioxidant Enzyme Activities and Plasma Brain Natriuretic Peptide Levels in Patients with Chronic Cor Pulmonale
Authors: Bayram E , Atalay C , Kocatürk H , Yücel O 
Published: February 2013

An oxidant/antioxidant imbalance in favour of oxidants appears to occur in chronic cor pulmonale (CCP). Oxidative stress could also be a critical event in the pathogenesis of this condition. Trimetazidine (TMZ) has antioxidant properties and may affect the utilization of oxygen radicals. We investigated the effect of TMZ (20 mg three times daily, orally) on activities of erythrocyte malondialdehyde (MDA) and catalase (eCAT), erythrocyte and plasma glutathione peroxidase (GSH-Px) and plasma superoxide dismutase (pSOD) in CCP patients. We also assessed changes in plasma levels of brain natriuretic peptide (BNP) with TMZ therapy. Sixty CCP patients with significantly higher MDA and markedly lower pSOD, eCAT and GSH-Px (erythrocyte) activities than 24 healthy controls were randomly allocated to receive routine treatment or routine treatment plus TMZ. After 3 months' therapy, greater pSOD, eCAT and GSH-Px (erythrocyte and plasma) activities and lower MDA activity were found with TMZ treatment compared with routine treatment. Plasma BNP levels were significantly lower in TMZ-treated patients and higher in the routine treatment group than in the control group. TMZ improved antioxidant levels, decreased oxidative stress and decreased plasma BNP levels in CCP patients.
The Effects of Interferon α2b on Chemically-Induced Peritoneal Fibrosis and on Peritoneal Tissue MMP-2 and TIMP-2 Levels in Rats
Authors: Ucar E , Borazan A , Semerci E , Binici DN , Yaldiz M , Aysal A , Altug E , Kuvandik C , Huzmeli C , Yetim T , Canda S 
Published: February 2013

This study investigated the effect of interferon α2b on chlorhexidine gluconate (CH)-induced peritoneal fibrosis (PF) in rats and assessed peritoneal tissue levels of metalloproteinase (MMP)-2 and tissue inhibitors of metalloproteinases (TIMP)-2. Wistar albino rats ( n = 8 per group) were treated as follows: control group, 3 ml/day of 0.9% saline intra-peritoneally for 28 days; CH group, 0.1% CH (200 g [3 ml]/day) in 15% ethanol and 0.9% saline intra-peritoneally for 28 days; CH + interferon (IFN) group, CH (as above) plus pegylated IFN-α2b 1.5 μg/kg per week subcutaneously on days 0, 7, 14, 21 and 28; IFN group, pegylated IFN-α2b (as above). Parietal peritoneum samples were obtained from the left anterior abdominal wall after 35 days. Parietal thickness, degree of vascular proliferation and inflammation, and MMP-2 and TIMP-2 levels were determined. The mean peritoneal thicknesses of the control, CH, CH + IFN and IFN groups were 7.02 ± 3.89, 156.86 ± 29.13, 59.88 ± 22.1, 9.27 ± 2.03 μm, respectively. Pegylated IFN-α2b decreased CH-induced expression of MMP-2 in the parietal peritoneum, but had no effect on TIMP-2 levels. Further studies are needed to determine the optimal dosage and duration for pegylated IFN-α2b treatment.
Neuroprotective Effect of Gypenosides against Oxidative Injury in the Substantia Nigra of a Mouse Model of Parkinson's Disease
Authors: Wang P , Niu L , Gao L , Li W-X , Jia D , Wang X-L , Gao G-D 
Published: February 2013

Oxidative injury has been implicated in the aetiology of Parkinson's disease (PD) and gypenosides (GP), which are saponins with various bioactivities, have shown antioxidative effects in vitro. The present study was designed to evaluate the effect of GP on a 1-methyl-4-phenyl-1,2,3, 6-tetrahydropyridine (MPTP)-induced mouse model of PD. Acute administration of MPTP led to decreased glutathione content and reduced superoxide dismutase activity in the substantia nigra of the mice, which resulted in oxidative stress, loss of nigral dopaminergic neurons and motor dysfunction. Co-treatment with GP attenuated all the injuries induced by MPTP in a dose-dependent manner. The neuroprotective effect of GP may be attributed to increased antioxidation, as manifested by significantly increased glutathione content and enhanced superoxide dismutase activity in the substantia nigra. These results strongly indicate the possible therapeutic potential of GP as an antioxidant in PD.
The relationship of trigger finger and flexor tendon volar migration after carpal tunnel release
Authors: Lee S. K. , Bae K. W. , Choy W. S. 
Published: February 2013

It has been suggested that the increased frequency of trigger finger (TF) after carpal tunnel release (CTR) may be caused by the volar migration of the flexor tendons at the wrist altering the tendon biomechanics at the A1 pulley. This hypothesis has not been validated. We performed pre- and post-operative ultrasonography (USG) on the affected wrists of 92 patients who underwent CTR. Pre-operative USG was performed in neutral with no tendon loading; post-operative USG was performed in neutral unloaded and in various positions of wrist flexion whilst loading the flexor tendons with gripping. The mean volar migration of the flexor tendons after CTR was 2.2 (SD 0.4) mm in the unloaded neutral position. It was 1.8 (SD 0.4) mm in patients who did not develop TF and 2.5 (SD 0.5) mm in those who did ( p = 0.0067). In loaded wrist flexion, the mean volar migration of flexor tendons after CTR in patients who did not develop TF and those who did was 2.1 and 3.0 mm in 0° flexion; 3.2 and 3.9 mm in 15° flexion; 4.3 and 5.1 mm in 30° flexion; and 4.9 and 5.8 mm in 45° flexion, respectively. There were significant differences between patients with and without TF at each flexion angle. Our data indicate that patients with greater volar migration of the flexor tendons after CTR are more likely to develop TF. This conclusion supports the hypothesis that the occurrence of TF after CTR may be caused by the bowstringing effects of the flexor tendons.
Lisfranc Injury in a West Point Cadet
Authors: Diebal Angela R. , Westrick Richard B. , Alitz Curtis , Gerber J. Parry 
Published: February 2013

Background: Lisfranc joint injuries are fairly uncommon; however, few injuries hold such an elevated potential for devastating chronic secondary pain and disability. It is imperative when evaluating an injury to the ankle or foot to have a high clinical index of suspicion for Lisfranc injury, as physical examination findings are often subtle. Case Description: An 18-year-old military cadet reported to a direct-access sports physical therapy clinic with foot pain. Despite negative radiographic findings, there was a high suspicion for Lisfranc injury based on the injury mechanism and physical examination. A computed tomography scan demonstrated an oblique fracture through the base of the third metatarsal, a small marginal fracture at the plantar base of the second metatarsal, and a subtle diastasis. Two days following his injury, he underwent an open reduction and internal fixation, completed subsequent rehabilitation, and returned to full activity approximately 1 year following injury. Conclusion: Early diagnosis of Lisfranc injuries is imperative for proper management and prevention of a poor functional outcome. If a strong clinical suspicion exists, negative radiographic findings are insufficient to rule out a Lisfranc injury, and therefore, advanced imaging is required.
Foxp3+ T-regulatory cells require DNA methyltransferase 1 expression to prevent development of lethal autoimmunity
Authors: Wang Liqing , Liu Yujie , Beier Ulf H. , Han Rongxiang , Bhatti Tricia R. , Akimova Tatiana , Hancock Wayne W. 
Published: February 2013

Key Points
Foxp3 expression is increased by DNMT inhibitors and may have potential utility in efforts to develop Foxp3+ Tregs for cellular therapy. Dnmt1 deletion impairs Treg function and results in lethal autoimmunity, such that use of Dnmt inhibitors may warrant careful consideration.
PDK1 regulates platelet activation and arterial thrombosis
Authors: Chen Xue , Zhang Yue , Wang Yanhua , Li Ding , Zhang Lin , Wang Kemin , Luo Xinping , Yang Zhongzhou , Wu Yi , Liu Junling 
Published: February 2013

Key Points
PDK1 is involved in thrombin-induced platelet activation and αIIbβ3-mediated outside-in signaling by regulating the downstream effector Gsk3β.
EB1, p150Glued, and Clasp1 control endothelial tubulogenesis through microtubule assembly, acetylation, and apical polarization
Authors: Kim Dae Joong , Martinez-Lemus Luis A. , Davis George E. 
Published: February 2013

Key Points
Microtubule tip complex proteins control EC lumen formation through tubulin acetylation to support the developing apical membrane surface. Microtubule assembly and tubulin modifications interface with proteolytic and kinase cascades to control EC lumen formation in 3D matrices.
Galectin-1 serum levels reflect tumor burden and adverse clinical features in classical Hodgkin lymphoma
Authors: Ouyang Jing , Plütschow Annette , von Strandmann Elke Pogge , Reiners Katrin S. , Ponader Sabine , Rabinovich Gabriel A. , Neuberg Donna , Engert Andreas , Shipp Margaret A. 
Published: February 2013

Key Points
Serum levels of galectin-1 are significantly higher in patients with cHL than in normal controls. Galectin-1 serum levels are associated with clinical parameters of tumor burden in patients with cHL.
Nearly n-homomorphisms and n-derivations in fuzzy ternary Banach algebras
Authors: Hassani Feysal , Ebadian Ali , Eshaghi Gordji Madjid , Azadi Kenary Hassan 
Published: February 2013

Abstract
Let

n
=
3
k
+
2

for some

k
∈
N

. We investigate the generalized Hyers-Ulam stability of n-homomorphisms and n-derivations on fuzzy ternary Banach algebras related to the generalized Cauchy-Jensen additive functional equation.

MSC:39B52, 46S40, 26E50.
Management of stage II colon cancer - the use of molecular biomarkers for adjuvant therapy decision
Authors: Donada Marisa , Bonin Serena , Barbazza Renzo , Pettirosso Daniel , Stanta Giorgio 
Published: February 2013

Abstract

Background
There is uncertainty on the benefit of adjuvant chemotherapy in patients with stage II colorectal cancers. The aim of this study is to investigate the combined role of clinical, pathological and molecular parameters to identify those stage II patients who better benefit from adjuvant therapy.

Methods
We examined 120 stage II colon cancer patients. Of these, 60 patients received adjuvant 5-FU chemotherapy after surgery and the other 60 did not receive therapy. Immunohistochemical (IHC) analyses were performed to evaluate the expressions of Thymidylate synthetase (TYMS), TP53 (p53), β-catenin (CTNNB1) and CD8. For TYMS, its mRNA expression levels were also investigated by real time qRT-PCR. The entire case study was characterized by the presence of a defect in the MMR (mismatch repair) system, the presence of the CpG island methylator phenotype (CIMP or CIMP-High) and for the V600E mutation in the BRAF gene. At the histo-pathological level, the depth of tumour invasion, lymphovascular invasion, invasion of large veins, host lymphocytic response and tumour border configuration were recorded.

Results
The presence of the V600E mutation in the BRAF gene was a poor prognostic factor for disease free and overall survival (DFS; hazard ratio [HR], 2.57; 95% CI: 1.03 -6.37; p = 0.04 and OS; HR, 3.68; 95% CI: 1.43-9.47; p < 0.01 respectively), independently of 5-FU treatment. Adjuvant therapy significantly improved survival in patients with high TYMS levels (p = 0.04), while patients with low TYMS had a better outcome if treated by surgery alone (DFS; HR, 6.07; 95% CI, 0.82 to 44.89; p = 0.04). In patients with a defect in the MMR system (dMMR), 5-FU therapy was associated to reduced survival (DFS; HR, 37.98; 95% CI, 1.04 to 1381.31; p = 0.04), while it was beneficial for CIMP-High associated tumours (DFS; HR, 0.17; 95% CI, 0.02 to 1.13; p = 0.05).

Conclusions
Patients’ characterization according to MMR status, CIMP phenotype and TYMS mRNA expression may provide a more tailored approach for adjuvant therapy in stage II colon cancer.
Spread of Influenza A(H1N1) oseltamivir-resistant viruses in Africa in 2008 confirmed by multiple introductions in Senegal
Authors: Dia Ndongo , Niang Mbayame N , Diadhiou Saadiya A , Goudiaby Déborah G , Faye Abdourahmane , Kiori Davy , Bâ Mady , Michel Rémy , Diop Ousmane M 
Published: February 2013

AbstractBackgroundAmong Influenza neuraminidase inhibitors (NAIs), oseltamivir corresponds to the most widely used agent to treat influenza disease. However since 2001, several cases of resistance to NAIs have been reported for circulating seasonal A(H1N1) Influenza viruses. A direct resistance mechanism may be invoked, involving critical mutations in the viral NA gene that prevent the drug binding to its target. Same phenomenon is reported for adamantanes drugs and mutations in the M2 channel protein gene of Influenza viruses.MethodsReverse-Transcription/Restriction Fragment Length Polymorphism (RT-PCR/RFLP) method, phenotypic testing for oseltamivir resistance, and sequencing of NA, HA and M2 genes were used in this study. Phylogenetic analyses were performed using BioEdit and Mega 5 softwares for alignment of sequences and phylogenetic trees building respectively.ResultsUsing a simple RT-PCR/RFLP method, we found that the 86 seasonal A(H1N1) isolates from 2008 bear the oseltamivir resistance-associated mutation (H274Y) in the NA gene. In contrast all isolates isolated in Senegal in 2007 were sensitive to oseltamivir. These results were first confirmed by finding high IC50 values using a phenotypic testing for oseltamivir resistance, and secondly by sequencing the whole NA gene. Regarding M2 gene, no mutation associated to adamantanes resistance was characterized of the isolates.ConclusionsThe present work provides evidence of circulation of drug-resistant seasonal A(H1N1) viruses during the 2008 influenza season (July to September) in Senegal. The results are in favor of multiple introductions of oseltamivir resistant viruses (ORV) A(H1N1) in Senegal.Phylogenetic analyses of isolates with complete sequences of N1 and HA1 genes showed that they belong to clade 2B and suggest sequential introductions in Africa.