bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of two‐component system AfsQ1/Q2 regulon and its cross‐regulation with GlnR in Streptomyces coelicolor
Authors: Wang Rui , Mast Yvonne , Wang Jin , Zhang Weiwen , Zhao Guoping , Wohlleben Wolfgang , Lu Yinhua , Jiang Weihong 
Published: October 2012

SummaryThe two‐component system AfsQ1/Q2 of Streptomyces coelicolor was identified in our previous work as a pleiotropic regulator for antibiotic biosynthesis and morphological differentiation under the condition of a minimal medium supplemented with 75 mM glutamate. In this work, we report the dissection of the mechanism underlying the function of AfsQ1/Q2 on antibiotic production and also the identification of the AfsQ1/Q2 regulon. The results showed that AfsQ1/Q2 stimulated antibiotic ACT, RED and CDA production directly through the pathway‐specific activator genes actII‐ORF4, redZ and cdaR respectively. In addition, expression of sigQ that encodes a sigma factor and is divergently transcribed from afsQ1 was also subject to direct regulation by AfsQ1/Q2. The precise AfsQ1 binding sites in the upstream regions of these target genes were determined by DNase I footprinting assays coupled with site‐directed DNA mutagenesis. By computational prediction and functional analysis, at least 17 new AfsQ1 targets were identified, including pstS gene encoding a high‐affinity phosphate‐binding protein and two developmental genes whiD, bldM. For the AfsQ1/Q2 regulon, an AfsQ1 binding motif comprising the sequence GTnAC‐n6‐GTnAC has been defined. Interestingly, we found from electrophoretic mobility shift assays and transcriptional analysis that AfsQ1/Q2 can also function as a repressor for nitrogen assimilation, and AfsQ1 can compete with GlnR for the promoter regions of glnA and nirB, suggesting the cross‐regulation between AfsQ1/Q2 and GlnR in nitrogen metabolism. These findings suggested that AfsQ1/Q2 is important not only for antibiotic biosynthesis but also in maintaining the metabolic homeostasis of nutrient utilization under the stress of high concentration of glutamate in S. coelicolor.
The velvet complex governs mycotoxin production and virulence of Fusarium oxysporum on plant and mammalian hosts
Authors: López‐Berges Manuel S. , Hera Concepción , Sulyok Michael , Schäfer Katja , Capilla Javier , Guarro Josep , Di Pietro Antonio 
Published: October 2012

SummaryFungal pathogens provoke devastating losses in agricultural production, contaminate food with mycotoxins and give rise to life‐threatening infections in humans. The soil‐borne ascomycete Fusarium oxysporum attacks over 100 different crops and can cause systemic fusariosis in immunocompromised individuals. Here we functionally characterized VeA, VelB, VelC and LaeA, four components of the velvet protein complex which regulates fungal development and secondary metabolism. Deletion of veA, velB and to a minor extent velC caused a derepression of conidiation as well as alterations in the shape and size of microconidia. VeA and LaeA were required for full virulence of F. oxysporum on tomato plants and on immunodepressed mice. A critical contribution of velvet consists in promoting chromatin accessibility and expression of the biosynthetic gene cluster for beauvericin, a depsipeptide mycotoxin that functions as a virulence determinant. These results reveal a conserved role of the velvet complex during fungal infection on plants and mammals.
Emotion regulation in delusion‐proneness: Deficits in cognitive reappraisal, but not in expressive suppression
Authors: Westermann Stefan , Rief Winfried , Lincoln Tania Marie 
Published: October 2012

ObjectivesAlthough anxiety plays a key role in delusions, its downregulation using specific emotion regulation (ER) strategies has not been investigated. Reappraisal has been shown to be one of the most effective strategies for healthy individuals. However, individuals with delusions might have difficulties in successfully applying reappraisal. This study therefore tests the effectiveness of reappraisal compared to expressive suppression in individuals with varying levels of delusion‐proneness.DesignAn experimental design with the independent variables ER strategy (within subject) and delusion‐proneness (between subject; quasi‐experimental) was used. The dependent variables were subjective ER success and physiological arousal, as well as state delusional ideation.MethodsEighty‐six healthy participants with different levels of delusion‐proneness were instructed to respond to anxiety‐inducing stimuli by either using reappraisal or expressive suppression.ResultsOverall, reappraisal was more effective than expressive suppression in regulating anxiety. However, delusion‐prone individuals were less successful in applying reappraisal (interaction effect: F(2,158) = 3.70, p = .027). In addition, lower success in reappraising threat was accompanied by higher state delusional ideation (r = −0.20, p = .013).ConclusionsDelusion‐proneness is accompanied by difficulties in reappraising threat that might contribute to the formation and maintenance of clinically relevant delusions. Preliminary implications for the improvement of cognitive behaviour therapy for delusions are discussed.Practitioner Points

Delusion‐proneness is accompanied by difficulties in applying the emotion regulation strategy ‘reappraisal’ to anxiety.
The concept of emotion regulation might be useful in individualized disorder models in CBT for psychosis.
Expression of Aquaporin Water Channels in Equine Endometrium is Differentially Regulated During the Oestrous Cycle and Early Pregnancy
Authors: Klein C , Troedsson MHT , Rutllant J 
Published: October 2012

ContentsThe expression of 12 different aquaporin subtypes in equine endometrium was examined at the mRNA and protein level. Endometrial samples were obtained during anoestrus, oestrus, 8, and 14 days after ovulation in non‐pregnant mares, and 14 days after ovulation in pregnant mares. Quantitative PCR revealed a time‐dependent pattern for all aquaporin subtypes examined except for AQP10 and 12. AQP3, 5 and 7 showed highest mRNA abundance 8 days after ovulation, while AQP0 and 2 were most abundant at Day 14 of the cycle in non‐pregnant mares. At 14 days of pregnancy, AQP1, 4, 8, 9 and 11 displayed highest expression levels. Western blot analysis confirmed protein expression of AQP0, 2 and 5. Immunohistochemistry localized protein expression to luminal and glandular epithelial and stromal cells. AQP0 staining intensity was highest in samples obtained on Day 14 of the oestrous cycle. AQP2 immunoreactivity seemed to be stronger in samples collected 14 days after ovulation from non‐pregnant animals, in particular luminal epithelial staining. Samples collected 8 days after ovulation from cyclic animals were characterized by intense AQP5 staining of glandular epithelium, predominantly in the deeper glands. Progesterone treatment of anoestrous mares did not enhance expression of AQPs, indicating that factors other than progesterone are required for the up‐regulation of certain AQP subtypes during dioestrus. In conclusion, it seems that an equine‐specific collaboration of aquaporin subtypes contributes to changes in endometrial fluid content occurring throughout the oestrous cycle and contributes to endometrial receptivity during early pregnancy in the mare.
Genome Sequence of Nitratireductor aquibiodomus Strain RA22
Authors: Singh Ajit , Jangir Pramod Kumar , Kumari Chanchal , Sharma Rakesh 
Published: October 2012

ABSTRACT

The genus
Nitratireductor
represents nitrate-reducing bacteria from the family
Phyllobacteriaceae
. Here we report the draft genome sequence of
Nitratireductor aquibiodomus
strain RA22, which contains 4,592,790 bp, with a G+C content of 61.30%, and has 4,241 protein coding genes.
Whole-Genome Sequence of Nocardiopsis alba Strain ATCC BAA-2165, Associated with Honeybees
Authors: Qiao Jianjun , Chen Lei , Li Yongli , Wang Jiangxin , Zhang Weiwen , Chen Shawn 
Published: October 2012

ABSTRACT

The actinomycete
Nocardiopsis alba
was reportedly associated with honeybees in separate occurrences. We report the complete genome of
Nocardiopsis alba
ATCC BAA-2165 isolated from honeybee guts. It will provide insights into the metabolism and genetic regulatory networks of this genus of bacteria that enable them to live in a range of environments.
Genome Sequence of “Candidatus Mycoplasma haemolamae” Strain Purdue, a Red Blood Cell Pathogen of Alpacas (Vicugna pacos) and Llamas (Lama glama)
Authors: Guimaraes Ana M. S. , Toth Balazs , Santos Andrea P. , do Nascimento Naíla C. , Kritchevsky Janice E. , Messick Joanne B. 
Published: October 2012

ABSTRACTWe report the complete genome sequence of “CandidatusMycoplasma haemolamae,” an endemic red-cell pathogen of camelids. The single, circular chromosome has 756,845 bp, a 39.3% G+C content, and 925 coding sequences (CDSs). A great proportion (49.1%) of these CDSs are organized into paralogous gene families, which can now be further explored with regard to antigenic variation.
Whole-Genome Sequence of Cupriavidus sp. Strain BIS7, a Heavy-Metal-Resistant Bacterium
Authors: Hong Kar Wai , Thinagaran Dinaiz a/l , Gan Han Ming , Yin Wai-Fong , Chan Kok-Gan 
Published: October 2012

ABSTRACT

Cupriavidus
sp. strain BIS7 is a Malaysian tropical soil bacterium that exhibits broad heavy-metal resistance [Co(II), Zn(II), Ni(II), Se(IV), Cu(II), chromate, Co(III), Fe(II), and Fe(III)]. It is particularly resistant to Fe(II), Fe(III), and Zn(II). Here we present the assembly and annotation of its genome.
Genome Sequence of Oscillibacter ruminantium Strain GH1, Isolated from Rumen of Korean Native Cattle
Authors: Lee Gun-Hye , Kumar Sanjay , Lee Jong-Hwan , Chang Dong-Ho , Kim Dae-Soo , Choi Sang-Haeng , Rhee Moon-Soo , Lee Dong-Woo , Yoon Min-Ho , Kim Byoung-Chan 
Published: October 2012

ABSTRACT

Oscillibacter ruminantium
strain GH1 was isolated from the rumen of Korean native cattle (HanWoo;
Bos taurus coreanae
). Here, we present the 3.07-Mb draft genome of this strain, which could reveal the presence of certain fiber-specific glycoside hydrolases and butyric acid-producing genes.
Heavy-Metal Resistance of a France Vineyard Soil Bacterium, Pseudomonas mendocina Strain S5.2, Revealed by Whole-Genome Sequencing
Authors: Chong Teik Min , Yin Wai-Fong , Mondy Samuel , Grandclément Catherine , Dessaux Yves , Chan Kok-Gan 
Published: October 2012

ABSTRACT

Here we present the draft genome of
Pseudomonas mendocina
strain S5.2, possessing tolerance to a high concentration of copper. In addition to being copper resistant, the genome of
P. mendocina
strain S5.2 contains a number of heavy-metal-resistant genes known to confer resistance to multiple heavy-metal ions.
A Systematic Review of Cost-Effectiveness Analyses Alongside Randomised Controlled Trials of Acupuncture
Authors: Kim Song-Yi , Lee Hyangsook , Chae Younbyoung , Park Hi-Joon , Lee Hyejung 
Published: October 2012

Objective To summarise the evidence on the cost-effectiveness of acupuncture. Methods We identified full economic evaluations such as cost-effectiveness analysis (CEA), cost-utility analysis (CUA) and cost-benefit analysis (CBA) alongside randomised controlled trials (RCTs) that assessed the consequences and costs of acupuncture for any medical condition. Eleven electronic databases were searched up to March 2011 without language restrictions. Eligible RCTs were assessed using the Cochrane criteria for risk of bias and a modified version of the checklist for economic evaluation. The general characteristics and the results of each economic analysis such as incremental cost-effectiveness ratios (ICERs) were extracted. Results Of 17 included studies, nine were CUAs that measured quality-adjusted life years (QALYs) and eight were CEAs that assessed effectiveness of acupuncture based on improvements in clinical symptoms. All CUAs showed that acupuncture with or without usual care was cost-effective compared with waiting list control or usual care alone, with ICERs ranging from ¢3011/QALY (dysmenorrhoea) to ¢22 298/QALY (allergic rhinitis) in German studies, and from £3855/QALY (osteoarthritis) to £9951/QALY (headache) in UK studies. In the CEAs, acupuncture was beneficial at a relatively low cost in six European and Asian studies. All CUAs were well-designed with a low risk of bias, but this was not the case for CEAs. Conclusions Overall, this review demonstrates the cost-effectiveness of acupuncture. Despite such promising results, any generalisation of these results needs to be made with caution given the diversity of diseases and the different status of acupuncture in the various countries.
Usefulness of parental response to questions about adherence to prescribed inhaled corticosteroids in young children
Authors: Schultz André , Sly Peter D , Zhang Guicheng , Venter André , Devadason Sunalene G , le Souëf Peter Niels 
Published: October 2012

BackgroundAdherence to prescribed inhaled medication is often low in young children. Poor adherence to medication may contribute to lack of symptom control. Doctors are not good at predicting the adherence rates of their patients, and parental report of adherence does not correlate with objective measures of adherence. The objective of this study was to investigate whether parental admission of non-adherence and reasons given for non-adherence correlated with objectively measured adherence.MethodsAdherence to prescribed inhaled corticosteroid treatment was monitored electronically in 132 children aged 2–6 years who were participating in a randomised controlled trial comparing different inhaler devices. Follow-up was carried out every 3 months for a year. Parental answers to simple questions about adherence were compared to electronically measured adherence.ResultsMean adherence ranged from zero to 100%. Intra-participant adherence varied throughout the year-long study period (mean variance for individual children between quarterly periods was 28.5%). Parents who reported missed doses, generally missed at least half of the prescribed doses. Parents who reported that not a single prescribed dose was missed, still missed 20% of doses on average. Adherence was particularly low when parents cited initiating their own trial off medication as a reason for missing doses.ConclusionsBy examining parental response to questions enquiring whether any doses were missed, healthcare providers can gain a modest degree of insight into their patients’ true adherence to prescribed medication. Adherence to prescribed asthma medication is extremely variable in young children.Trial registration numberData from this study were derived from a randomised controlled trial (ACTRN12608000294358).
Number and burden of cardiovascular diseases in relation to health-related quality of life in a cross-sectional population-based cohort study
Authors: Djärv Therese , Wikman Anna , Lagergren Pernilla 
Published: October 2012

ObjectivesTo clarify whether a greater number of cardiovascular diseases or a larger burden of disease are associated with poorer health-related quality of life (HRQoL) in an unselected general population.DesignA population-based cross-sectional postal survey.SettingsA random sample of the Swedish general population aged 40–79 years matched for national distributions of age, gender and region.ParticipantsOut of 6969 eligible individuals, 4910 (70.5%) participated.Primary and secondary measuresTo create a reference database for HRQoL outcomes in the general population. To assess certain diseases and their relation to HRQoL.MethodsPredefined cardiovascular diseases and HRQoL were assessed from validated questionnaires (EORTC QLQ-C30). Aspects of HRQoL included in the analyses were global quality of life, physical function, role function, emotional function, fatigue and dyspnoea. Individuals were categorised into: ‘good function’ versus ‘poor function’ and ‘no or minor symptoms’ versus ‘symptomatic’. Multivariable logistic regression calculated OR with 95% CI for poor HRQoL. The exposures were the number of cardiovascular diseases and the subjective disease burden.ResultsOut of the 4910 participants, 1358 (28%) reported having a cardiovascular disease and hypertension was most common. Reporting a greater number of cardiovascular diseases was associated with an increased risk of poor HRQoL, especially regarding dyspnoea. The OR for symptomatic dyspnoea was 1.37 (95% CI 1.08 to 1.74) for participants with one cardiovascular disease, 4.81 (95% CI 3.24 to 7.13) for two diseases and 4.18 (95% CI 2.24 to 7.80) for those with three or more cardiovascular diseases. Among the 271 participants who assessed their cardiovascular disease burden as major, the highest risk for poor HRQoL was found for physical function (OR 6.18, 95% CI 3.72 to 10.30).ConclusionsIncreased number of cardiovascular diseases and a greater burden of disease are generally associated with poorer HRQoL in people with cardiovascular disease from an unselected population.
Ankle-Brachial Index determination and peripheral arterial disease diagnosis by an oscillometric blood pressure device in primary care: validation and diagnostic accuracy study
Authors: Nelson Mark R , Quinn Stephen , Winzenberg Tania M , Howes Faline , Shiel Louise , Reid Christopher M 
Published: October 2012

ObjectivesTo determine the level of agreement between a ‘conventional’ Ankle-Brachial Index (ABI) measurement (using Doppler and mercury sphygmomanometer taken by a research nurse) and a ‘pragmatic’ ABI measure (using an oscillometric device taken by a practice nurse) in primary care. To ascertain the utility of a pragmatic ABI measure for the diagnosis of peripheral arterial disease (PAD) in primary care.DesignCross-sectional validation and diagnostic accuracy study. Descriptive analyses were used to investigate the agreement between the two procedures using the Bland and Altman method to determine whether the correlation between ABI readings varied systematically. Diagnostic accuracy was assessed via sensitivity, specificity, accuracy, likelihood ratios, positive and negative predictive values, with ABI readings dichotomised and Receiver Operating Curve analysis using both univariable and multivariable logistic regression.SettingPrimary care in metropolitan and rural Victoria, Australia between October 2009 and November 2010.Participants250 persons with cardiovascular disease (CVD) or at high risk (three or more risk factors) of CVD.ResultsDespite a strong association between the two method's measurements of ABI there was poor agreement with 95% of readings within ±0.4 of the 0.9 ABI cut point. The multivariable C statistic of diagnosis of PAD was 0.89. Other diagnostic measures were sensitivity 62%, specificity 92%, positive predictive value 67%, negative predictive value 90%, accuracy 85%, positive likelihood ratio 7.3 and the negative likelihood ratio 0.42.ConclusionsOscillometric ABI measures by primary care nurses on a population with a 22% prevalence of PAD lacked sufficient agreement with conventional measures to be recommended for routine diagnosis of PAD. This pragmatic method may however be used as a screening tool high-risk and overt CVD patients in primary care as it can reliably exclude the condition.
Conducting a multicentre and multinational qualitative study on patient transitions
Authors: Johnson Julie K , Barach Paul , Vernooij-Dassen Myrra ,   on behalf of the HANDOVER Research Collaborative
Published: October 2012

BackgroundA multicentre, multinational research study requires careful planning and coordination to accomplish the aims of the study and to ensure systematic and rigorous examination of all project methods and data collected.ObjectiveThe aim of this paper is to describe the approach we used during the HANDOVER Project to develop a multicentre, multinational research project for studying transitions of patient care while creating a community of practice for the researchers.MethodWe highlight the process used to assure the quality of a multicentre qualitative study and to create a codebook for data analysis as examples of attending to the community of practice while conducting rigorous qualitative research.FindingsEssential elements for the success of this multinational, multilanguage research project included recruiting a strong research team, explicit planning for decision-making processes to be used throughout the project, acknowledging the differences among the study settings and planning the protocols to capitalise upon those differences.ConclusionsAlthough not commonly discussed in reports of large research projects, there is an underlying, concurrent stream of activities to develop a cohesive team that trusts and respects one another's skills and that engage independent researchers in a group process that contributes to achieving study goals. We discuss other lessons learned and offer recommendations for other teams planning multicentre research.
Relational mechanisms and psychological outcomes in couples affected by breast cancer: a systematic narrative analysis of the literature
Authors: Hubbard Gill , Menzies Sandra , Flynn Pamela , Adams Sally , Haseen Farhana , Thomas Ian , Scanlon Karen , Reed Liz , Forbat Liz 
Published: October 2012

IntroductionRelationships are a significant dimension of illness experience. At the couple level, partners will respond to illness as an interpersonal unit rather than individuals in isolation. Research adopting a relational perspective have focused on communication, relational coping and relationship functioning and satisfaction. To our knowledge, there is no published systematic review of literature that reports associations between a couple's relationship and psychological outcomes of patients and partners affected by breast cancer.AimTo review studies that examine the impact of relational mechanisms on psychological outcomes in couples affected by breast cancer and thereby improve understanding of the connections between patient, disease and family.MethodsA systematic search for literature was conducted, which was followed by a thematic analysis of study findings and a narrative synthesis.ResultsSixteen papers were included. Papers were published relatively recently between 1988 and 2010. Three relational components were identified: (i) couple coping, (ii) relationship functioning and satisfaction, (iii) communication. While the literature indicates associations between relational and psychological variables, with such a small evidence base, the use of different terminology and different theoretical frameworks makes it almost impossible to draw definitive conclusions about which relational component holds greatest potential for effecting change on psychological well-being.ConclusionsWhile there remain many opportunities for contributing to the theoretical and empirical work in this field, there is sufficient evidence to propose a relational approach to supporting people affected by cancer.