bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technological Heredity and Accuracy of the Cross-Section Shapes of the Hydro-Cylinder Cylindrical Surfaces
Authors: Kryvyi Petro D. , Dzyura Volodymyr O. , Tymoshenko Nadiya M. , Krupa Volodymyr V. 
Published: October 2014

It was proposed to estimate the accuracy of the hydro-cylinder cross-section shapes basing on the analysis of the circle diagram of their inner cylindrical surfaces, formed by the manufacturing operations, such as rough and semi-finishing boring and finishing paying-out, taking advantage of the method of deflection approximation from the circle by the Fourier’s trigonometric series in the gap [0, 2π].
Characteristics of the deflection dispersion of the radius-vector as the random value (average value, dispersion, amplitude spectrum) have been obtained for every circle diagram separately. It is proposed to present these characteristics as random values.
Dependences for finding mathematic expectation and dispersion for the lot of parts, machined at every of the available manufacturing operation, have been obtained for certain number of samples and circle diagrams, taking advantage of the small sampling theory, the method of rectangular engagement in particular.
The effectiveness of manufacturing operations has been estimated according to the Student and Fisher t-test. Availability of the advantageous or disadvantageous manufacturing heredity of the hydrocylinder cross-section shape has been found.
As the result of carried out experimental investigations and processing of the obtained statistic data on the inner cylindrical surface of the truck hoist crane hydrocylinder KTA-25, produced by the company “Drogobych truck crane plant” (Ukraine), the need to improve the manufacturing operation of the semi-finishing boring has been analysed.
Microstructure-Based 3D FE Modeling for Micro Cutting Ferritic-Pearlitic Carbon Steels
Authors: Abouridouane M. , Klocke F. , Lung D. 
Published: October 2014

The mechanics of the cutting process on the microscopic level differ fundamentally from the conventional macro cutting. For example, the tool edge radius influences the cutting mechanism in micro machining significantly with regard to the effective rake angle, the minimum chip thickness, the dominance of ploughing, and the related elasto-plastic deformation of the workpiece material. These phenomena, known as size effects, have a profound impact on the cutting force, process stability, and resulting surface finish in micro cutting. Therefore, microstructural effects in microscale cutting require quite different assumptions to be made concerning underlying material behaviour during micro cutting and have led to the need for new modeling approaches to account for such effects.
This paper presents a three-dimensional finite element approach to incorporate microstructure into micro cutting simulation based on the concept of a representative volume element (RVE) and constitutive material modeling as well as using the Lagrangian formulation proposed in the implicit FE code Deform 3D™. Micro drilling and micro milling tests using solid carbide tools with different diameters (d = 50 μm − 1 mm) were performed on ferrite-pearlite two-phase steel AISI 1045 for the verification of the developed 3D multiphase FE computation model regarding chip formation, feed force, and torque. The developed 3D multiphase FE model was successfully used to predict size effects in micro cutting.
Fatigue Evaluation of 8630 Cast Steel Subjected to Post-Weld Heat Treatment and Stress Relieving
Authors: Shirazi Alireza , Ragai Ihab 
Published: October 2014

The effect of post weld heat treating and stress relieving on the fatigue strength of AISI 8630 cast steel, weld repaired with different filler materials, is the primary objective of the study. To determine the material properties, experiments included monotonic tensile tests, load-controlled fatigue tests as well as hardness tests. Moreover, specimens were micro-etched to examine the morphology of the fracture surface.
The results of the fatigue tests are presented in the form of S-N charts. The test findings are then employed in a generalized numerical solution to predict the fatigue behavior of similar components.
Finite element models are used to calculate stresses in tested samples, stress concentrations, and in fatigue life comparisons. Stress-life predictions were performed using the modified Goodman criterion to account for the mean stress effects caused by the stress ratio R = 0.1 loading. Predictions based off of finite element analysis and analytical solution for fatigue life provided reasonable estimates which are confirmed by the experimental results.
Draft Genome Sequences of Brucella suis Biovar 4 Strain NCTC 10385, Brucella ceti Strain NCTC 12891
T
, Brucella inopinata Strain CAMP 6436
T
, and Brucella neotomae Strain ATCC 23459
T
Authors: Wahab Tara , Ferrari Sevinc , Lindberg Martina , Bäckman Stina , Kaden Rene 
Published: October 2014

ABSTRACT

With the aim of developing quantitative PCR methods for the detection and differentiation of
Brucella
species, the genomes of
Brucella ceti
,
Brucella inopinata
,
Brucella netotomae
, and
Brucella suis
biovar 4 were sequenced and analyzed.
Complete Genome Sequence of Equid Herpesvirus 3
Authors: Sijmons Steven , Vissani Aldana , Tordoya Maria Silva , Muylkens Benoît , Thiry Etienne , Maes Piet , Matthijnssens Jelle , Barrandeguy Maria , Van Ranst Marc 
Published: October 2014

ABSTRACT

Equid herpesvirus 3 (EHV-3) is a member of the subfamily
Alphaherpesvirinae
that causes equine coital exanthema. Here, we report the first complete genome sequence of EHV-3. The 151,601-nt genome encodes 76 distinct genes like other equine alphaherpesviruses, but genetically, EHV-3 is significantly more divergent.
Draft Genome Sequence of Polychlorinated Biphenyl-Dechlorinating Dehalococcoides mccartyi Strain SG1, Which Carries a Circular Putative Plasmid
Authors: Wang Shanquan , Chng Kern Rei , Wu Chen , Wilm Andreas , Nagarajan Niranjan , He Jianzhong 
Published: October 2014

ABSTRACT

Dehalococcoides mccartyi
strain SG1, isolated from digester sludge, dechlorinates polychlorinated biphenyls (PCBs) to lower congeners. Here we report the draft genome sequence of SG1, which carries a 22.65 kbp circular putative plasmid.
Draft Genome Sequences of Campylobacter fetus subsp.
venerealis
bv. venerealis Strain B6 and bv. intermedius Strain 642-21
Authors: Barrero Roberto A. , Moolhuijzen Paula , Indjein Léa , Venus Bronwyn , Keeble-Gagnère Gabriel , Power John , Bellgard Matthew I. , Lew-Tabor Ala E. 
Published: October 2014

ABSTRACT

Campylobacter fetus
subsp.
venerealis
is an important venereal pathogen. We sequenced the genomes of
Campylobacter fetus
subsp.
venerealis
bv. venerealis strain B6 and bv. intermedius strain 642-21. The genetic variability of these Australian strains will facilitate the study of mechanisms of geographical adaptation of these pathogens that impact livestock.
Draft Genome Assembly of Pseudomonas aeruginosa Quality Control Reference Strain Boston 41501
Authors: Minogue T. D. , Daligault H. E. , Davenport K. W. , Broomall S. M. , Bruce D. C. , Chain P. S. , Coyne S. R. , Gibbons H. S. , Jaissle J. , Chertkov O. , Freitas T. , Rosenzweig C. N. , Xu Y. , Johnson S. L. 
Published: October 2014

ABSTRACT

We present the scaffolded genome assembly of
Pseudomonas aeruginosa
Boston 41501, now publicly available in GenBank (JOVK00000000) in 10 contigs placed into a single scaffold. The 6.82-Mbp genome contains 66.1% G+C content and 6,295 coding sequences, including type 4 pilus and type 3 secretion system production genes.
Genome Sequence of Lactococcus lactis subsp.
lactis
NCDO 2118, a GABA-Producing Strain
Authors: Oliveira Letícia C. , Saraiva Tessália D. L. , Soares Siomar C. , Ramos Rommel T. J. , Sá Pablo H. C. G. , Carneiro Adriana R. , Miranda Fábio , Freire Matheus , Renan Wendel , Júnior Alberto F. O. , Santos Anderson R. , Pinto Anne C. , Souza Bianca M. , Castro Camila P. , Diniz Carlos A. A. , Rocha Clarissa S. , Mariano Diego C. B. , de Aguiar Edgar L. , Folador Edson L. , Barbosa Eudes G. V. , Aburjaile Flavia F. , Gonçalves Lucas A. , Guimarães Luís C. , Azevedo Marcela , Agresti Pamela C. M. , Silva Renata F. , Tiwari Sandeep , Almeida Sintia S. , Hassan Syed S. , Pereira Vanessa B. , Abreu Vinicius A. C. , Pereira Ulisses P. , Dorella Fernanda A. , Carvalho Alex F. , Pereira Felipe L. , Leal Carlos A. G. , Figueiredo Henrique C. P. , Silva Artur , Miyoshi Anderson , Azevedo Vasco 
Published: October 2014

ABSTRACT

Lactococcus lactis
subsp.
lactis
NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of
L. lactis
NCDO 2118, a strain with probiotic potential activity.
Draft Genome Sequences of
Devosia
sp. Strain 17-2-E-8 and Devosia riboflavina Strain IFO13584
Authors: Hassan Yousef I. , Lepp Dion , He Jianwei , Zhou Ting 
Published: October 2014

ABSTRACT

Here we report the draft genome of
Devosia
sp. strain 17-2-E-8, isolated from Ontario agricultural soil (Canada) with promising deoxynivalenol biotransformation capabilities. In addition, we report the draft genome of
Devosia riboflavina
strain IFO13584, used as a control strain in our studies aimed at highlighting unique gene clusters involved in deoxynivalenol epimerization.
High-Quality Draft Genome Sequence of
Pseudomonas
sp. BRG100, a Strain with Bioherbicidal Properties against
Setaria viridis
(Green Foxtail) and Other Pests of Agricultural Significance
Authors: Dumonceaux Tim J. , Town Jennifer , Links Matthew G. , Boyetchko Sue 
Published: October 2014

ABSTRACT

Pseudomonas
sp. BRG100 inhibits the growth of certain agricultural pests and is a potentially useful biopesticide for weeds and plant diseases. We have sequenced the 6.25-Mbp genome of this strain and assembled it into 4 scaffolds. Genome sequence comparisons revealed that this strain may represent a novel species of
Pseudomonas
.
Habitual alcohol consumption associated with reduced semen quality and changes in reproductive hormones; a cross-sectional study among 1221 young Danish men
Authors: Jensen Tina Kold , Gottschau Mads , Madsen Jens Otto Broby , Andersson Anne-Maria , Lassen Tina Harmer , Skakkebæk Niels E , Swan Shanna H , Priskorn Lærke , Juul Anders , Jørgensen Niels 
Published: October 2014

ObjectiveStudy associations between three measures of alcohol consumption (recent, typical/habitual, binging), semen quality and serum reproductive hormones.DesignCross-sectional population based study.Setting and participants1221 young Danish men, aged 18–28 years were recruited when they attended a compulsory medical examination to determine their fitness for military service from 2008 to 2012. Total alcohol consumption: (1) in the week preceding (habitual/typical) the visit (recent alcohol intake), (2) in a typical week and (3) frequency of ‘binge drinking’ (consuming more than 5 units/day)) in the past 30 days was estimated.Main outcome measuresSemen quality (volume, sperm concentration, total sperm count, and percentages of motile and morphologically normal spermatozoa) and serum concentration of reproductive hormones (follicle-stimulating hormone, luteinising hormone, testosterone, sex hormone binding globulin, oestradiol, free testosterone and inhibin B).ResultsSperm concentration, total sperm count and percentage of spermatozoa with normal morphology were negatively associated with increasing habitual alcohol intake. This association was observed in men reporting at least 5 units in a typical week but was most pronounced for men with a typical intake of more than 25 units/week. Men with a typical weekly intake above 40 units had a 33% (95% CI 11% to 59%) reduction in sperm concentration compared to men with an intake of 1–5 units/week. A significant increase in serum free testosterone with increasing alcohol consumption the week preceding the visit was found. Binging was not independently associated with semen quality.ConclusionsOur study suggests that even modest habitual alcohol consumption of more than 5 units per week had adverse effects on semen quality although most pronounced associations were seen in men who consumed more than 25 units per week. Alcohol consumption was also linked to changes in testosterone and SHBG levels. Young men should be advised to avoid habitual alcohol intake.
Phase Field Modeling of Nonequilibrium Patterns on the Surface of a Liquid Film Under Lateral Oscillations at the Substrate
Authors: Borcia Rodica , Bestehorn Michael 
Published: October 2014

We use a phase field model which couples the generalized Navier–Stokes equation (including the Korteweg stress tensor) with the continuity equation for studying nonlinear pattern formation on the surface of a liquid film under (linear and circular) lateral harmonic vibrations at the solid substrate. First, we prove the thermodynamic consistency of our phase field model. Next, we present computer simulations in three spatial dimensions. We illustrate nonequilibrium patterns at the instability onset, confirming in this way the results recently reported in Phys. Rev. E 88, 023025 (2013). The lateral profiles of the deflected surface are compared with those reported in J. Fluid Mech. 686, 409 (2011) for Faraday instability excited by vertical harmonic vibrations at the bottom plate.
Phase I, Dose-Escalation Study of the Targeted Cytotoxic LHRH Analog AEZS-108 in Patients with Castration- and Taxane-Resistant Prostate Cancer
Authors: Liu Stephen V. , Tsao-Wei Denice D. , Xiong Shigang , Groshen Susan , Dorff Tanya B. , Quinn David I. , Tai Yu-Chong , Engel Juergen , Hawes Debra , Schally Andrew V. , Pinski Jacek K. 
Published: October 2014

Abstract
Purpose: AEZS-108, formerly AN-152, is a cytotoxic hybrid molecule consisting of a luteinizing hormone-releasing hormone (LHRH) agonist moiety covalently coupled to doxorubicin, allowing it to deliver doxorubicin selectively to cells expressing LHRH receptors. LHRH receptors are expressed on the cell membrane of many tumors, including prostate cancer. This phase I study determined the maximum tolerated dose (MTD) of AEZS-108 in men with taxane- and castration-resistant prostate cancer (CRPC) while providing additional information on the safety profile and efficacy of this agent.
Experimental Design: AEZS-108 was administered as an intravenous infusion every 21 days until progression or unacceptable toxicity in cohorts of 3 or 6 patients until the MTD was reached. Blood was collected for capture of circulating tumor cells (CTC) to visualize internalization of AEZS-108, an autofluorescent molecule.
Results: The MTD of AEZS-108 in this cohort was 210 mg/m2, which was lower than that seen in a phase I study conducted in women with endometrial or ovarian cancers. The dose-limiting toxicity was persistent neutropenia. Three patients had a PSA response with an additional 10 patients maintaining PSA stable disease. Of the 10 patients evaluable by RECIST criteria, 9 achieved stable disease. AEZS-108 internalization in CTCs was routinely visualized using its autofluorescence.
Conclusion: These findings show that AEZS-108 has an acceptable safety profile and a signal of efficacy, lowering PSA in heavily pretreated patients with prostate cancer, and that internalization of AEZS-108 in prostate cancer CTCs may be a viable pharmacodynamic marker. A phase II study in men with prostate cancer is ongoing. Clin Cancer Res; 20(24); 6277–83. ©2014 AACR.
Pilot Clinical Trial of Hedgehog Pathway Inhibitor GDC-0449 (Vismodegib) in Combination with Gemcitabine in Patients with Metastatic Pancreatic Adenocarcinoma
Authors: Kim Edward J. , Sahai Vaibhav , Abel Ethan V. , Griffith Kent A. , Greenson Joel K. , Takebe Naoko , Khan Gazala N. , Blau John L. , Craig Ronald , Balis Ulysses G. , Zalupski Mark M. , Simeone Diane M. 
Published: October 2014

Abstract
Purpose: The hedgehog (HH) signaling pathway is a key regulator in tumorigenesis of pancreatic adenocarcinoma and is upregulated in pancreatic adenocarcinoma cancer stem cells (CSCs). GDC-0449 is an oral small-molecule inhibitor of the HH pathway. This study assessed the effect of GDC-0449–mediated HH inhibition in paired biopsies, followed by combined treatment with gemcitabine, in patients with metastatic pancreatic adenocarcinoma.
Experimental Design: Twenty-five patients were enrolled of which 23 underwent core biopsies at baseline and following 3 weeks of GDC-0449. On day 29, 23 patients started weekly gemcitabine while continuing GDC-0449. We evaluated GLI1 and PTCH1 inhibition, change in CSCs, Ki-67, fibrosis, and assessed tumor response, survival and toxicity.
Results: On pretreatment biopsy, 75% of patients had elevated sonic hedgehog (SHH) expression. On posttreatment biopsy, GLI1 and PTCH1 decreased in 95.6% and 82.6% of 23 patients, fibrosis decreased in 45.4% of 22, and Ki-67 in 52.9% of 17 evaluable patients. No significant changes were detected in CSCs pre- and postbiopsy. The median progression-free and overall survival for all treated patients were 2.8 and 5.3 months. The response and disease control rate was 21.7% and 65.2%. No significant correlation was noted between CSCs, fibrosis, SHH, Ki-67, GLI1, PTCH1 (baseline values or relative change on posttreatment biopsy), and survival. Grade ≥3 adverse events were noted in 56% of patients.
Conclusion: We show that GDC-0449 for 3 weeks leads to downmodulation of GLI1 and PTCH1, without significant changes in CSCs compared with baseline. GDC-0449 and gemcitabine were not superior to gemcitabine alone in the treatment of metastatic pancreatic cancer. Clin Cancer Res; 20(23); 5937–45. ©2014 AACR.
Promising Cell-Based Immunotherapy Using Gamma Delta T Cells: Together Is Better
Authors: Déchanet-Merville Julie 
Published: October 2014

Abstract
Gamma delta T-cell response to cellular stress signals expressed by tumor cells makes them promising candidates for cancer immunotherapy. The proof of concept for clinical scale propagation of polyclonal γδ T-cell lines with efficient in vitro and in vivo response against cancer is an important step in this direction. Clin Cancer Res; 20(22); 5573–5. ©2014 AACR.
PET Imaging of β-Glucuronidase Activity by an Activity-Based 124I-Trapping Probe for the Personalized Glucuronide Prodrug Targeted Therapy
Authors: Su Yu-Cheng , Cheng Ta-Chun , Leu Yu-Ling , Roffler Steve R. , Wang Jaw-Yuan , Chuang Chih-Hung , Kao Chien-Han , Chen Kai-Chuan , Wang Hsin-Ell , Cheng Tian-Lu 
Published: October 2014

Abstract
Beta-glucuronidase (βG) is a potential biomarker for cancer diagnosis and prodrug therapy. The ability to image βG activity in patients would assist in personalized glucuronide prodrug cancer therapy. However, whole-body imaging of βG activity for medical usage is not yet available. Here, we developed a radioactive βG activity–based trapping probe for positron emission tomography (PET). We generated a 124I-tyramine–conjugated difluoromethylphenol beta-glucuronide probe (TrapG) to form 124I-TrapG that could be selectively activated by βG for subsequent attachment of 124I-tyramine to nucleophilic moieties near βG-expressing sites. We estimated the specificity of a fluorescent FITC-TrapG, the cytotoxicity of tyramine-TrapG, and the serum half-life of 124I-TrapG. βG targeting of 124I-TrapG in vivo was examined by micro-PET. The biodistribution of 131I-TrapG was investigated in different organs. Finally, we imaged the endogenous βG activity and assessed its correlation with therapeutic efficacy of 9-aminocamptothecin glucuronide (9ACG) prodrug in native tumors. FITC-TrapG showed specific trapping at βG-expressing CT26 (CT26/mβG) cells but not in CT26 cells. The native TrapG probe possessed low cytotoxicity. 124I-TrapG preferentially accumulated in CT26/mβG but not CT26 cells. Meanwhile, micro-PET and whole-body autoradiography results demonstrated that 124I-TrapG signals in CT26/mβG tumors were 141.4-fold greater than in CT26 tumors. Importantly, Colo205 xenografts in nude mice that express elevated endogenous βG can be monitored by using infrared glucuronide trapping probes (NIR-TrapG) and suppressed by 9ACG prodrug treatment. 124I-TrapG exhibited low cytotoxicity allowing long-term monitoring of βG activity in vivo to aid in the optimization of prodrug targeted therapy. Mol Cancer Ther; 13(12); 2852–63. ©2014 AACR.
Small Molecule BMH-Compounds That Inhibit RNA Polymerase I and Cause Nucleolar Stress
Authors: Peltonen Karita , Colis Laureen , Liu Hester , Jäämaa Sari , Zhang Zhewei , af Hällström Taija , Moore Henna M. , Sirajuddin Paul , Laiho Marikki 
Published: October 2014

Abstract
Activation of the p53 pathway has been considered a therapeutic strategy to target cancers. We have previously identified several p53-activating small molecules in a cell-based screen. Two of the compounds activated p53 by causing DNA damage, but this modality was absent in the other four. We recently showed that one of these, BMH-21, inhibits RNA polymerase I (Pol I) transcription, causes the degradation of Pol I catalytic subunit RPA194, and has potent anticancer activity. We show here that three remaining compounds in this screen, BMH-9, BMH-22, and BMH-23, cause reorganization of nucleolar marker proteins consistent with segregation of the nucleolus, a hallmark of Pol I transcription stress. Further, the compounds destabilize RPA194 in a proteasome-dependent manner and inhibit nascent rRNA synthesis and expression of the 45S rRNA precursor. BMH-9– and BMH-22–mediated nucleolar stress was detected in ex vivo–cultured human prostate tissues indicating good tissue bioactivity. Testing of closely related analogues showed that their activities were chemically constrained. Viability screen for BMH-9, BMH-22, and BMH-23 in the NCI60 cancer cell lines showed potent anticancer activity across many tumor types. Finally, we show that the Pol I transcription stress by BMH-9, BMH-22, and BMH-23 is independent of p53 function. These results highlight the dominant impact of Pol I transcription stress on p53 pathway activation and bring forward chemically novel lead molecules for Pol I inhibition, and, potentially, cancer targeting. Mol Cancer Ther; 13(11); 2537–46. ©2014 AACR.
Pulmonary Sarcoid–like Granulomatosis after Multiple Vaccinations of a Long-term Surviving Patient with Metastatic Melanoma
Authors: Bordry Natacha , Costa-Nunes Carla-Marisa , Cagnon Laurène , Gannon Philippe O. , Abed-Maillard Samia , Baumgaertner Petra , Murray Timothy , Letovanec Igor , Lazor Romain , Bouchaab Hasna , Rufer Nathalie , Romano Emanuela , Michielin Olivier , Speiser Daniel E. 
Published: October 2014

Abstract
Autoimmune side effects are frequent in patients with cancer treated with immune checkpoint–targeting antibodies, but are rare with cancer vaccines. Here, we present a case report on a patient with metastatic melanoma who developed pulmonary sarcoid–like granulomatosis following repetitive vaccinations with peptides and CpG. Despite multiple metastases, including one lesion in the brain, the patient is alive and well more than 13 years after the diagnosis of metastatic disease. The strongly activated tumor-specific CD8+ T cells showed robust long-term memory and effector functions. It is possible that long-term survival and adverse autoimmune events may become more common for vaccines inducing robust anticancer immune responses as were present in this patient. Cancer Immunol Res; 2(12); 1148–53. ©2014 AACR.