bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microhabitat and adhesive toepads shape gecko limb morphology
Authors: ZIMIN Anna , ZIMIN Sean V. , GRISMER L. Lee , BAUER Aaron M. , CHAPPLE David G. , DEMBITZER Jacob , ROLL Uri , MEIRI Shai 
Published: August 2024

AbstractDifferent substrates pose varied biomechanical challenges that select specific morphologies, such as long limbs for faster running and short limbs for balanced posture while climbing narrow substrates. We tested how gecko locomotion is affected by the microhabitat they occupy and by a key adaptation—adhesive toepads—through analyzing how those are related to limb morphology. We collected microhabitat and toepads data for over 90% of limbed gecko species, and limb measurements for 403 species from 83 of the 121 limbed gecko genera, which we then used in phylogenetic comparative analyses. Our data highlight the association of adhesive toepads with arboreality, but a phylogenetic analysis shows that this relationship is not significant, suggesting that these traits are phylogenetically constrained. Comparative analyses reveal that pad‐bearing species possess shorter hindlimbs and feet, more even limb lengths, and lower crus: thigh ratios, than padless geckos, across microhabitats. Saxicolous geckos have the longest limbs and limb segments. This is probably influenced by selection for long strides, increased takeoff velocity, and static stability on inclined surfaces. Terrestrial geckos have more even hind‐ and forelimbs than arboreal geckos, unlike patterns found in other lizards. Our findings underline the difficulty to infer on microhabitat–morphology relationships from one taxon to another, given their differing ecologies and evolutionary pathways. We emphasize the importance of key innovation traits, such as adhesive toepads, in shaping limb morphology in geckos and, accordingly, their locomotion within their immediate environment.
Stabilized chlorophyll‐based food colorants from spinach: Kinetics of a tailored enzymatic extraction
Authors: Lombardelli Claudio , Mazzocchi Caterina , Benucci Ilaria , Esti Marco 
Published: August 2024

AbstractAn organic solvent‐free method based on limited dosing options (biocatalyst and zinc chloride) for the quick and mild recovery of chlorophyll (Chl) from spinach has been proposed. This tailored, custom‐made protocol has been designed to produce stable green natural colorants. The kinetics of pigment extraction turned out to be a very useful tool to identify the proper conditions, in terms of biocatalyst dose (0.10–50 U/g), extraction time (1–48h), and ZnCl2 amount (50–300ppm), both for enhancing the recovery yield and preserving the green color. Considering the extraction kinetics, the recovery yield, and the colorimetric data, the suitable conditions to produce stable green and food‐grade colorants are 0.10 U/g of enzyme, 3h, and 150ppm of ZnCl2 at 25°C. The extraction yield of Chl (4863µg/U) was about 51% greater than control, with a higher extraction rate constant (5.43 × 10−4 g/(µg min)). Considering the impact of ZnCl2 amount on Chl, its protective action resulted to be more noticeable toward Chl a: at 150ppm, an increased amount of about 2.5 and 1.5 times was found for Chl a and Chl b, respectively, in comparison to the reference (0ppm ZnCl2).Practical ApplicationThis research demonstrates how a suitable kinetic approach helps to provide a tailored protocol, customized for the vegetable matrix, to produce stable green natural colorants from spinach. Lowering enzyme dosage and ZnCl2 amount during the extraction of chlorophyll at low temperature is crucial for its potential use as a colorant in food industry, providing high economic values through saving time and environmental protection.
Expropriation as reparation
Authors: Chan Shuk Ying 
Published: August 2024

AbstractWith some recent exceptions, demands for global reparations have largely been ignored by former colonial countries. While the past two decades has seen renewed interest in colonial reparations in normative political theory and philosophy, this work has focused on determining responsibility for redress. By contrast, relatively little has been said on the further question of how redress might be sought in face of persistent colonial amnesia and apologia. This paper defends expropriation—unilateral public takeovers of ownership and/or control of foreign assets—as a justified response to overdue colonial reparations. In making this case, the paper (1) moves our focus beyond questions of responsibility for reparative justice to consider what victims of past injustice (and/or their descendants) are justified in doing to obtain their due and (2) explores distinctive issues that arise for political resistance at the global level.
Trajectories of egg sensitization in childhood: Two birth cohorts in Asia and Europe
Authors: Nakamura Toshinori , Nakano Taiji , Simpson Angela , Kono Michihiro , Curtin John A. , Kobayashi Tomoko , Murray Clare S. , Akiyama Masashi , Imanishi Masahiro , Mikuriya Masayuki , Custovic Adnan , Shimojo Naoki 
Published: August 2024

AbstractBackgroundHen's egg exposure through impaired skin barrier is considered a major mechanism of sensitization to eggs. However, the impact of filaggrin (FLG) gene loss‐of‐function mutations on the natural history of egg sensitization lacks consensus among studies.ObjectiveTo evaluate the association between the natural course of egg sensitization and FLG mutations.MethodsWe used Japanese and the UK birth cohorts (CHIBA and MAAS) to identify the longitudinal patterns of egg sensitization until mid‐school age and examined the relationship between the identified patterns and FLG mutations. Sensitization was assessed using egg white‐specific IgE levels or skin prick tests (SPTs). Egg allergy was confirmed by parental reports and sensitization. Latent class growth analysis identified longitudinal patterns.ResultsThree similar patterns of egg sensitization (persistent, early‐onset remitting, and no/low grade classes) were identified in both cohorts, with differing prevalence estimates. The proportion of children with egg allergy in the persistent class at 7 or 8 years of age was 23% (CHIBA) and 20% (MAAS). Consistently in both cohorts, FLG mutations were significantly associated only with the persistent class. Children with FLG mutations had an approximately four‐fold increased risk of being in the persistent sensitization class (RRRs: 4.3, 95%C.I. (1.2–16.0), p = .03 in CHIBA; 4.3 (1.3–14.7), p = .02 in MAAS).ConclusionFLG loss‐of‐function mutations are associated with persistent egg sensitization in both Japanese and European ethnicities, and the mutations might be a potential biomarker for identifying the risk of persistent egg sensitization/allergy in early infancy. Future studies should incorporate oral food challenges to confirm this relationship.
Effectiveness of pharmacological procedural sedation in children with autism spectrum disorder: A systematic review and meta‐analysis
Authors: Zupin Luisa , Sahyoun Cyril , Krauss Baruch , Dagri Arianna , Rocco Elisabetta Maria , Barbi Egidio , Celsi Fulvio 
Published: August 2024

AbstractAimManagement of primary healthcare and routine minor procedures for children with autism spectrum disorder (ASD) can be challenging; therefore, when behavioural strategies fail, sedative medications are often employed. We evaluated the effectiveness of the current pharmacological strategies for managing children with ASD.MethodsWe performed a systematic review and meta‐analysis of the current approaches for procedural sedation in children with ASD.ResultsTwenty studies met inclusion criteria. Dexmedetomidine, midazolam, propofol and chloral hydrate were the most efficient agents for successful procedures, while propofol had the highest number of adverse events. The most frequently used agents were dexmedetomidine and midazolam or a combination of the two, and the effectiveness of dexmedetomidine plus midazolam was superior to dexmedetomidine alone.ConclusionMultiple effective drug regimens exist for procedural sedation in children with ASD. These results could support the development of specific guidelines for procedural sedation in children with ASD.
When political elites talk, citizens reply. Affective polarization through temporal orientation and intergroup emotions
Authors: Garzón‐Velandia Diana Camila , Barreto‐Galeano María Idaly , Sabucedo‐Cameselle José Manuel 
Published: August 2024

AbstractPolitical polarization on social media, particularly during electoral campaigns, has become a growing concern. This study aimed to assess levels of affective polarization in political communication, considering temporal orientation, delegitimizing beliefs, and intergroup emotions. Two studies were conducted: one during the Andalusian elections in Spain, and another during the Colombian presidential campaign. Tweets from candidates and X users were analyzed in both studies. Linguistic analysis was used to develop an index for measuring affective polarization in linguistic pieces. This index offers an alternative to the lack of linguistic measurement tools for psychological processes regarding political polarization. Findings showed that communicative strategies often exhibited high indicators of ingroup bias in contexts without political violence, resulting in lower polarization that increased with positive emotions and a forward‐looking perspective. Conversely, in contexts of political violence, strategies shifted towards outgroup discrimination and delegitimization. Positive emotions decreased polarization in these situations, while a past focus intensified it. The study concluded that affective polarization could indicate the willingness to pursue reconciliation in violent contexts. Consequently, this research provides a map of emotions associated with polarization.Public significance statementTwo studies conducted in Spain and Colombia analyzed how political figures and users of the social network X communicate during elections. It was identified that the use of emotions can contribute to political polarization in both populations. Positive emotions can exacerbate polarization by glorifying one's group, while negative emotions can fuel polarization through attacks on opponents.
The multifaceted nature of early vocabulary development: Connecting children's characteristics with parental input types
Authors: Göksun Tilbe , Aktan‐Erciyes Aslı , Karadöller Dilay Z. , Demir‐Lira Ö. Ece 
Published: August 2024

AbstractChildren need to learn the demands of their native language in the early vocabulary development phase. In this dynamic process, parental multimodal input may shape neurodevelopmental trajectories while also being tailored by child‐related factors. Moving beyond typically characterized group profiles, in this article, we synthesize growing evidence on the effects of parental multimodal input (amount, quality, or absence), domain‐specific input (space and math), and language‐specific input (causal verbs and sound symbols) on preterm, full‐term, and deaf children's early vocabulary development, focusing primarily on research with children learning Turkish and Turkish Sign Language. We advocate for a theoretical perspective, integrating neonatal characteristics and parental input, and acknowledging the unique constraints of languages.
Patient autonomy and shared decision‐making in the context of clinical trial participation
Authors: Dennstädt Fabio , Putora Paul Martin , Iseli Thomas , Treffers Theresa , Panje Cédric , Fischer Galina Farina 
Published: August 2024

AbstractAimsThis study aimed to explore how incorporating shared decision‐making (SDM) can address recruitment challenges in clinical trials. Specifically, it examines how SDM can align the trial process with patient preferences, enhance patient autonomy and increase active patient participation. Additionally, it identifies potential conflicts between SDM and certain clinical trial aspects, such as randomization or blinding, and proposes solutions to mitigate these issues.Materials and MethodsWe conducted a comprehensive review of existing literature on patient recruitment challenges in clinical trials and the role of SDM in addressing these challenges. We analysed case studies and trial reports to identify common obstacles and assess the effectiveness of SDM in improving patient accrual. Additionally, we evaluated three proposed solutions: adequate trial design, communication skill training and patient decision aids.ResultsOur review indicates that incorporating SDM can significantly enhance patient recruitment by promoting patient autonomy and engagement. SDM encourages physicians to adopt a more open and informative approach, which aligns the trial process with patient preferences and reduces psychological barriers such as fear and mental stress. However, implementing SDM can conflict with elements such as randomization and blinding, potentially complicating trial design and execution.DiscussionThe desire for patient autonomy and active engagement through SDM may clash with traditional clinical trial methodologies. To address these conflicts, we propose three solutions: redesigning trials to better accommodate SDM principles, providing communication skill training for physicians and developing patient decision aids. By focussing on patient wishes and emotions, these solutions can integrate SDM into clinical trials effectively.ConclusionShared decision‐making provides a framework that can promote patient recruitment and trial participation by enhancing patient autonomy and engagement. With proper implementation of trial design modifications, communication skill training and patient decision aids, SDM can support rather than hinder clinical trial execution, ultimately contributing to the advancement of evidence‐based medicine.
Retirement wealth, earnings risks, and intergenerational links
Authors: Shao Lei , Zhang Jie 
Published: August 2024

AbstractThis paper investigates the accumulation and distribution of retirement wealth in a dynastic model with earnings risks, longevity uncertainties, and borrowing constraints. It resolves the wealth indeterminacy problem across generations in dynastic families by introducing a transaction cost for intergenerational transfers. It captures the pattern of inter vivos transfers, the relationship between wealth and earnings, and wealth inequality in the US data. Social security lowers precautionary savings by redistributing income from families with high earnings or short‐lived parents to others, thus reducing investment, the growth rate in income per capita, inequality in retirees' consumption, and the wealth‐earnings correlation.
Selection of sheep skin bacteria to reduce blood feeding by biting midges under laboratory conditions
Authors: Brok Paula S. , Jost Stéphanie M. , Verhulst Niels O. 
Published: August 2024

AbstractBiting midges of the genus Culicoides (Diptera: Ceratopogonidae) are of huge veterinary importance, mainly as vectors of pathogens, such as Bluetongue virus. Currently, there are no effective methods to protect animals against biting midges, as insecticides have limited or short‐lived efficacy. Biting midges are attracted to hosts by carbon dioxide and by their body odours, which are mainly produced by skin bacteria. In humans, it has been shown that differences between individuals in attractiveness to mosquitoes is mediated by these skin bacterial volatiles. This opens the possibility to protect individuals from biting insects by supplementing their skin microbiome with probiotics. In this study, we investigated this approach by culturing sheep skin bacteria on various media and assessing their effects against field‐caught Culicoides (overwhelmingly Obsoletus group species) as well as laboratory‐reared Culicoides nubeculosus (Meigen). Aerococcus urinaeequi, Bacillus safensis, Bacillus subtilis, Jeotgalicoccus psychrophilus, Micrococcus sp. and Staphylococcus equorum were selected to be tested in a dual‐choice Y‐tube olfactometer, assessing their behavioural effects on biting midges. We revealed an avoidance effect on laboratory‐reared C. nubeculosus when testing B. safensis and B. subtilis. Bacillus safensis and Micrococcus sp. yielded significant repellent potential towards field‐caught Culicoides. These two candidates were subsequently tested in a membrane blood‐feeding assay. When the bacterial species B. safensis was applied to the membrane, a feeding reduction of 83% was observed with field‐caught Culicoides.
Retroperitoneal and lower extremities muscle bleeding in acquired haemophilia A (AHA): Risk factors and implications in disability and survival
Authors: Pardos‐Gea José , Benítez Olga 
Published: August 2024

AbstractAimTo assess risk factors of retroperitoneal and lower extremity musculoskeletal bleed in acquired haemophilia (AHA) and perform an objective assessment of disability and influence on survival.MethodsWe included 49 patients with AHA from November 2017 to May 2023. The occurrence of any retroperitoneal or/and lower extremities bleeding manifestation was investigated. On clinical follow‐up, we search for compressive femoral neuropathy and quadriceps amyotrophy. The lower extremity functional scale (LEFS) was carried out one year after the last bleeding event in all AHA patients.ResultsA 61.2% of patients in our AHA cohort presented with any retroperitoneal and/or lower extremities musculoskeletal manifestation. Those patients had higher percentage of major bleeding EACH2/ISTH criteria (90% vs. 57%, p = .01), needs of blood transfusions (86% vs. 57% of patients, p = .03), and haemostatic by‐pass products (90% vs. 63%, p = .02). Hypertension (HR 2.6, 95% CI 1.1–5.9, p = .02), presence of autoimmune disease (HR 13, 95% CI 1.7–99, p = .01), and inhibitor level > 20 BU (HR 2.6 95% CI 1.0–6.8, p = .04) significantly predicted retroperitoneal/lower extremities clinical manifestations. Most frequent sequelae were quad atrophy (30.6%) and femoral nerve palsy (20.4%). Quad atrophy and LEFS scores under 50 were associated with increased mortality (HR 3, 95% CI 1.1–8.6 and HR 12, 95% CI 3.3–45, respectively).ConclusionAHA with retroperitoneal/lower extremities bleeding involvement is of greater severity and shows high disability and worst survival outcomes. Quadriceps atrophy and LEFS scale scoring under 50 predicted mortality in our AHA patients.
Genotyping‐by‐sequencing informs conservation of Andean palms sources of non‐timber forest products
Authors: Peñafiel Loaiza Nicolás , Chafe Abigail H. , Moraes R Mónica , Oleas Nora H. , Roncal Julissa 
Published: August 2024

AbstractConservation and sustainable management of lineages providing non‐timber forest products are imperative under the current global biodiversity loss. Most non‐timber forest species, however, lack genomic studies that characterize their intraspecific variation and evolutionary history, which inform species' conservation practices. Contrary to many lineages in the Andean biodiversity hotspot that exhibit high diversification, the genus Parajubaea (Arecaceae) has only three species despite the genus' origin 22 million years ago. Two of the three palm species, P. torallyi and P. sunkha, are non‐timber forest species endemic to the Andes of Bolivia and are listed as IUCN endangered. The third species, P. cocoides, is a vulnerable species with unknown wild populations. We investigated the evolutionary relationships of Parajubaea species and the genetic diversity and structure of wild Bolivian populations. Sequencing of five low‐copy nuclear genes (3753 bp) challenged the hypothesis that P. cocoides is a cultigen that originated from the wild Bolivian species. We further obtained up to 15,134 de novo single‐nucleotide polymorphism markers by genotyping‐by‐sequencing of 194 wild Parajubaea individuals. Our total DNA sequencing effort rejected the taxonomic separation of the two Bolivian species. As expected for narrow endemic species, we observed low genetic diversity, but no inbreeding signal. We found three genetic clusters shaped by geographic distance, which we use to propose three management units. Different percentages of missing genotypic data did not impact the genetic structure of populations. We use the management units to recommend in situ conservation by creating new protected areas, and ex situ conservation through seed collection.
Sorting grid usage to reduce the catch of undersized narrow‐clawed crayfish (Pontastacus leptodactylus Eschscholtz, 1823) in fyke net fisheries
Authors: Cilbiz Mehmet , Aydın Celalettin 
Published: August 2024

AbstractSustainability of natural stocks of crayfish is critical because of their economic and ecological importance. Crayfish (Pontastacus leptodactylus), one of the most important species in Turkish inland fisheries, has been facing serious threats recently. One threat the species faces is high landed bycatch of undersized crayfish because of selective fishing gear, such as fyke nets. We estimated size‐selectivity of sorting grids with various bar spacings for crayfish to reduce bycatch. Six bar spacings (15.0, 17.5, 20.0, 22.5, 25.0, and 30.0 mm) were tested alongside commercial crayfish fyke nets. Mean selectivity (L50) (±95% CI) was 6.90 (6.63–6.99) cm for 15.0 mm bar spacing, 7.52 (7.16–8.02) cm for 17.5 mm, 8.52 (8.32–8.71) cm for 20.0 mm, 9.70 (9.28–9.90) cm for 22.5, 10.75 (10.34–11.17) cm for 25.0 mm, 11.80 (11.31–12.24) cm for 30.0 mm, and 8.79 (8.05–9.29) cm for commercial fyke nets. The proportion of undersized specimens caught be reduced from 32.27% for commercial fyke nets to as low as 2.46% by using sorting grids. The codend of 22.5 mm bar spacing was more selective, with significantly lower discard rates, and more effective for individuals above the minimum conservation reference size than commercial fyke nets.
Long‐term trends in the genotypic integrity, phenotype and reproductive development of perennial ryegrass (Lolium perenne L.) populations in New Zealand dairy pastures: Implications for pasture persistence
Authors: Chapman D. F. , Griffiths W. M. , Hofmann R. W. , Thomas E. , Faville M. J. , Kuhn‐Sherlock B. 
Published: August 2024

AbstractFailure of perennial ryegrass‐based pastures to persist beyond 3 years post‐sowing is an increasing threat to the sustainability of livestock industries in the north of New Zealand. Little is known of the long‐term responses of plants and populations to the environmental stresses responsible for persistence failure, or the effects of ryegrass genetics on those responses. Plants were collected from 10‐year‐old pastures sown in two environments (Waikato, North Island: warm‐temperate, summer/autumn dry; Canterbury, South Island: cool temperate, irrigated) to four ryegrass functional types and grazed by dairy cattle. Surviving plants in Canterbury pastures were almost all true‐to‐type for the original genotype, whereas there was substantial ingress of volunteer plants in the diploid cultivars in Waikato. Plants confirmed as true‐to‐type were compared to reference plants grown from the original seed lines. Leaf mass was lower in survivor plants than in reference plants for all functional types. This effect was reversed by recruitment of new plants from seed in a grazing deferment treatment applied at the Waikato site 18 months before plant collection, in a manner consistent with epigenetic control. Reproductive development was delayed by 4–6 days in survivors of mid‐ and late flowering diploid cultivars: this effect appeared to be the result of true genetic differentiation. There was less aftermath heading in the Canterbury survivors, but not Waikato survivors, compared with the reference plants. The relevance of these findings for ryegrass survival strategies and targeted selection of traits for improved persistence is discussed.