bluuebunny/crossref_metadata_2025_split huggingface dataset:
Operative utilization of balloon versus traditional endoscopic sinus surgery
Authors: Ference Elisabeth H. , Graber Madeline , Conley David , Chandra Rakesh K. , Tan Bruce K. , Evans Charlesnika , Pynnonen Melissa , Smith Stephanie S. 
Published: September 2014

Objectives/HypothesisTo study the utilization of balloon catheter dilation (BCD) compared to traditional endoscopic sinus surgery (ESS)Study DesignCross‐sectional analysisMethodsCases identified by Current Procedural Terminology codes as BCD (2,717) or traditional ESS (31,059) were extracted from the State Ambulatory Surgery Databases 2011 for California, Florida, Maryland, and New York. Patient demographics, surgical center and surgeon volume, mean charge, and operating room (OR) time were compared.ResultsThere were 33,776 patients who underwent sinus surgery in the included states in 2011. Of these, 4.6% of maxillary, 5.6% of sphenoid, and 13.9% of frontal procedures were performed using BCD. Adjusted analyses found increased use of BCD in patients with chronic diseases(P < .001). Patients who had limited sinus surgery were less likely to have BCD compared to patients who had all four sinuses instrumented (P < .001). Surgeons who performed a medium (odds ratio 1.38 [1.14–1.65]) or high (odds ratio 1.71 [1.42–2.07]) volume of ESSs were more likely to use BCD compared to those who performed a low volume (P < .001). However, among surgeons who utilized BCD, there was a minimal relationship between the percentage of surgeries performed with BCD and the surgeon's total number of cases (R2 = 0.055). Compared to traditional ESS, the median charges for maxillary/ethmoid procedures (mini‐ESS) involving BCD were approximately $4,500 (P < .001) and maxillary/ethmoid/sphenoid/frontal procedures (pan‐ESS) were approximately $2,950 (P = .003) greater, whereas the median OR time involving BCD was 8 minutes less for mini‐ESS procedures(P = .01) but not statistically different for pan‐ESS procedures (P = .58).ConclusionsIn the study sample, balloon technology was used in 8.0% of ESS cases in 2011. Procedures using BCD were on average more expensive compared to traditional ESS procedures, with minimal decrease in OR time.Level of Evidence2c Laryngoscope, 125:49–56, 2015
Detection of aneuploidy from single fetal nucleated red blood cells using whole genome sequencing
Authors: Hua Rui , Barrett Angela N. , Tan Tuan Zea , Huang Zhouwei , Mahyuddin Aniza Puteri , Ponnusamy Sukumar , Sandhu Jaspal Singh , Ho Sherry S. Y. , Chan Jerry K. Y. , Chong Samuel , Quan Song , Choolani Mahesh 
Published: September 2014

AbstractObjectiveThe objective of the study was to detect aneuploidy in single fetal nucleated red blood cells (FNRBCs) from placental villi using whole genome amplification (WGA) and next generation sequencing.MethodsThree single FNRBCs per sample were manually picked from villi collected from ten women undergoing elective first‐trimester termination of pregnancy, and one or two cells were picked from each of four aneuploid chorionic villus samples. Following WGA and addition of adaptor and index sequences, samples were sequenced on the Illumina MiSeq. Leading and trailing 15 bases were trimmed, and reads were aligned to the human reference genome. Z‐scores were calculated to determine deviation of the mean of the test from reference samples, with a score of 3 used as the threshold for classification of a particular chromosome as trisomic.ResultsWe successfully made correct diagnoses from ten single cells isolated from villi from two cases of trisomy 21 (one case from a single cell and one from two cells), two cases of trisomy 18 (two cells each), and a case of trisomy 15 (three cells).ConclusionWith their faithful representation of fetal genome, diagnosis using single FNRBCs provides a definitive result compared with non‐invasive prenatal testing using cell‐free fetal DNA, and is a safer alternative to invasive amniocentesis. © 2014 John Wiley & Sons, Ltd.
Isobenzofuranone derivatives exhibit antileishmanial effect by inhibiting type II DNA topoisomerase and inducing host response
Authors: Mishra Amartya , Vinayagam Jayaraman , Saha Sourav , Chowdhury Sayan , Roychowdhury Somenath , Jaisankar Parasuraman , Majumder Hemanta K. 
Published: September 2014

AbstractLeishmania, a protozoan parasite, causes a wide range of human diseases ranging from the localized self‐healing cutaneous lesions to fatal visceral leishmaniasis. Toxicity of traditional first line drugs and emergence of drug‐resistant strains have worsened the situation. DNA topoisomerase II in kinetoplastid protozoan parasites are of immense interest as drug target because they take part in replication of unusual kinetoplast DNA network. In this study, we have taken target‐based therapeutic approaches to combat leishmaniasis. Two isobenzofuranone compounds, viz., (1) 3,5‐bis(4‐chlorophenyl)‐7‐hydroxyisobenzofuran‐1(3H)‐one (JVPH3) and (2) (4‐bromo)‐3′‐hydroxy‐5′‐(4‐bromophenyl)‐benzophenone(JVPH4) were synthesized chemically and characterized by NMR and mass spectrometry analysis. Activity of type II DNA topoisomerase of leishmania (LdTOPII) was monitored by decatenation assay and plasmid cleavage assay. The antiparasitic activity of these compounds was checked in experimental BALB/c mice model of visceral leishmaniasis. Isobenzofuranone derivatives exhibited potent antileishmanial effect on both antimony (Sb) sensitive and resistant parasites. Treatment with isobenzofuranone derivatives on promastigotes caused induction of reactive oxygen species (ROS)‐mediated apoptosis like cell death in leishmania. Both the compounds inhibited the decatenation activity of LdTOPII but have no effect on bi‐subunit topoisomerase IB. Treatment of LdTOPII with isobenzofuranone derivatives did not stabilize cleavage complex formation both in vitro and in vivo. Moreover, treatment with isobenzofuranone derivatives on Leishmania donovani‐infected mice resulted in clearance of parasites in liver and spleen by induction of Th1 cytokines. Taken together, our data suggest that these compounds can be exploited as potential antileishmanial agents targeted to DNA topoisomerase II of the parasite.
Analysis of FK506, timcodar (VX‐853) and FKBP51 and FKBP52 chaperones in control of glucocorticoid receptor activity and phosphorylation
Authors: Hinds Terry D. , Stechschulte Lance A. , Elkhairi Fadel , Sanchez Edwin R. 
Published: September 2014

AbstractThe immunosuppressive ligand FK506 and the FK506‐binding protein FKBP52 are stimulatory to glucocorticoid receptor (GR) activity. Here, we explore the underlying mechanism by comparing GR activity and phosphorylation status in response to FK506 and the novel nonimmunosuppressive ligand timcodar (VX‐853) and in the presence and absence of FKBP52 and the closely related protein FKBP51. Using mouse embryonic fibroblast cells (MEFs) deficient knockout (KO) in FKBP51 or FKBP52, we show decreased GR activity at endogenous genes in 52KO cells, but increased activity in 51KO cells. In 52KO cells, elevated phosphorylation occurred at inhibitory serine 212 and decreased phosphorylation at the stimulatory S220 residue. In contrast, 51KO cells showed increased GR phosphorylation at the stimulatory residues S220 and S234. In wild‐type (WT) MEF cells, timcodar, like FK506, potentiated dexamethasone‐induced GR transcriptional activity at two endogenous genes. Using 52KO and 51KO MEF cells, FK506 potentiated GR activity in 51KO cells but could not do so in 52KO cells, suggesting FKBP52 as the major target of FK506 action. Like FK506, timcodar potentiated GR in 51KO cells, but it also increased GR activity in 52KO cells. Knock‐down of FKBP51 in the 52KO cells showed that the latter effect of timcodar required FKBP51. Thus, timcodar appears to have a dual specificity for FKBP51 and FKBP52. This work demonstrates phosphorylation as an important mechanism in FKBP control of GR and identifies the first nonimmunosuppressive macrolide capable of targeting GR action.
Use of a novel rapid and resource‐efficient cassette dosing approach to determine the pharmacokinetics and CNS distribution of small molecule 7‐transmembrane receptor allosteric modulators in rat
Authors: Bridges Thomas. M. , Morrison Ryan D. , Byers Frank W. , Luo Shuanghui , Scott Daniels J. 
Published: September 2014

AbstractApproaches to efficiently and accurately define the pharmacokinetics (PK) of large sets of small molecules in rodents have been previously described. Likewise, a variety of methods for determining brain tissue distribution (BTD) have been reported for use in the discovery of therapeutics targeting the central nervous system (CNS). Herein we describe a novel cassette approach to efficiently obtain concurrent PK and BTD data from a dose of up to five compounds in one rat over 24 h. In conjunction with fraction unbound (fu) data obtained in plasma and brain homogenate, this approach serves as an efficient means to determine compound unbound brain:unbound plasma partition coefficients (Kp,uu), thereby providing insight to compounds bearing poor permeability and/or active transporter activity impacting their permeation of the blood–brain barrier (BBB). This integrated approach was utilized in a lead optimization effort towards the discovery of CNS‐penetrant allosteric modulators of a seven‐transmembrane (7TM) receptor target. Rat PK and brain distribution was rapidly obtained for 70 compounds and correlated to data obtained from in vitro assessments. Two compounds that were evaluated in cassette and discrete studies, displayed agreement in PK (compound 1: cassette CLp = 1.6 mL min−1 kg−1, discrete CLp = 1.6 mL min−1 kg−1; compound 2: cassette CLp = 11 mL min−1 kg−1, discrete CLp = 8.1 mL min−1 kg−1) and BTD (compound 1: cassette Kp = 0.11, discrete Kp = 0.09; compound 2: cassette Kp < 0.05, discrete Kp = 0.04). The resulting data were used to guide medicinal chemistry efforts and to enable the progression of optimized compounds to in vivo pharmacodynamic assessments.
Proxy signcryption scheme in the standard model
Authors: Ming Yang , Wang Yumin 
Published: September 2014

AbstractProxy signcryption is a novel cryptographic primitive that combines the functionality of a proxy signature with that of a signcryption. However, the security of all the known proxy signcryption schemes were proven in the random oracle, which has received a lot of criticism that the proofs in the random oracle model are not sound with respect to the standard model. Hence, it is an interesting research problem on how to construct a provably secure proxy signcryption scheme in the standard model. In this paper, motivated by Waters' signature scheme, we propose a new construction of proxy signcryption scheme based on bilinear pairing without using random oracles. Then, we use the techniques from provable security to show that the proposed scheme is indistinguishability under adaptive chosen ciphertext attacks secure under the decisional bilinear Diffie–Hellman assumption and existentially unforgeable under the computational Diffie–Hellman assumptions. Compared with the existing schemes with formal security proof, we show that no pairing computation is required in our proxy signcrypt phase with pre‐computations. To the best of our knowledge, our proposed scheme is the first provably secure proxy signcryption scheme in the standard model. Copyright © 2014 John Wiley & Sons, Ltd.
Cold‐formed steel columns under L‐D‐G interaction
Authors: Souza dos Santos Eliane , de Miranda Batista Eduardo , Camotim Dinar 
Published: September 2014

AbstractThe paper addresses an experimental programme that is part of an ongoing investigation on the behaviour, strength and design of cold‐formed steel lipped channel columns undergoing mode interaction involving local, distortional and global buckling. The 16 column specimens tested were selected to exhibit either (i) close local, distortional and critical buckling loads (PcrL ≈︁ PcrD ≈︁ PcrG) or (ii) PcrL ≈︁ PcrD (condition ensured via the cross‐sectional dimensions) and PcrG up to around 20 % below or above that value (difference controlled via the column length). The test set‐up, procedure and results are presented and discussed, and the failure load data obtained are used to explore the possibility of developing a design approach based on the direct strength method (DSM).
CHD7 Maintains Neural Stem Cell Quiescence and Prevents Premature Stem Cell Depletion in the Adult Hippocampus
Authors: Jones Kieran M. , Sarić Nemanja , Russell John P. , Andoniadou Cynthia L. , Scambler Peter J. , Basson M. Albert 
Published: September 2014

Abstract
Neural stem/progenitor cells (NSCs) in the hippocampus produce new neurons throughout adult life. NSCs are maintained in a state of reversible quiescence and the failure to maintain the quiescent state can result in the premature depletion of the stem cell pool. The epigenetic mechanisms that maintain this quiescent state have not been identified. Using an inducible knockout mouse model, we show that the chromatin remodeling factor chromodomain–helicase-DNA-binding protein 7 (CHD7) is essential for maintaining NSC quiescence. CHD7 inactivation in adult NSCs results in a loss of stem cell quiescence in the hippocampus, a transient increase in cell divisions, followed by a significant decline in neurogenesis. This loss of NSC quiescence is associated with the premature loss of NSCs in middle-aged mice. We find that CHD7 represses the transcription of several positive regulators of cell cycle progression and is required for full induction of the Notch target gene Hes5 in quiescent NSCs. These findings directly link CHD7 to pathways involved in NSC quiescence and identify the first chromatin-remodeling factor with a role in NSC quiescence and maintenance. As CHD7 haplo-insufficiency is associated with a range of cognitive disabilities in CHARGE syndrome, our observations may have implications for understanding the basis of these deficits. Stem Cells 2015;33:196–210
Nonlinear hysteretic behavior simulation of reinforced concrete shear walls using the force analogy method
Authors: Li Gang , Zhang Feng , Zhang Yu , Li Hong‐Nan 
Published: September 2014

SummaryReinforced concrete (RC) shear walls that provide high strength and stiffness are widely used in buildings to resist lateral loads. It often exhibits complex and multiple hysteretic behaviors, including shear behavior, flexural behavior, strength softening, and deterioration, which are often influenced by the reinforcement and concrete material characteristics, vertical loads, and so on. Significant effort has gone toward developing accurate and efficient models, and these models fall into two categories: macroscopic and microscopic models. The microscopic models are accurate but computationally expensive and need more material information and experimental results for calibration. The present research is to implement an existing macroscopic model for use in the force analogy method, which is an accurate, efficient, and stable algorithm for conducting dynamic analysis when coupled with the state space formulation. The RC shear wall model with two vertical sliding hinges and one horizontal sliding hinge assigned to capture the relationship of the lateral deflection or rotation versus the RC shear wall force is validated against prior experimental results. The proposed model is also implemented in a frame, in which inelastic response occurs in both the frame and the RC shear wall members, to demonstrate the application of the model and the potential for simulating complex inelastic dynamic RC frame‐wall structural behavior with the force analogy method. Copyright © 2014 John Wiley & Sons, Ltd.
A study of biplanar crossed pin construct in the management of displaced pediatric supracondylar humeral fractures
Authors: Mohammed Riazuddin , Bhogadi Prabhudheer , Metikala Sreenivasulu 
Published: September 2014

Purpose Study of a biplanar crossed pin construct by two crossed Kirschner wires in the management of displaced extension type supracondylar humeral fractures in children. Methods Sixty-four patients with such fractures were included and treated according to the study protocol: after achieving closed reduction under general anesthesia with fluoroscopic control, two crossed Kirschner wires of equal diameter were inserted percutaneously. The first lateral wire tracts from the posterolateral corner of the epicondyle to the anteromedial cortex proximally. Then, the medial wire is inserted from the anteromedial corner of the epicondyle to the posterolateral cortex proximally after crossing the fracture site. Thus, a biplanar crossed pin construct was achieved, as each wire had two separate fixation points and the crossed construct was achieved not only in the coronal plane but also in the sagittal plane. Every effort was made to get this construct right at the very first attempt without repetition. Results Two patients were lost to follow-up during the first postoperative year. The mean follow-up for the remaining 62 patients was 14.5 months (range 6–24 months). At the final follow-up, using Flynn's overall modified classification, the clinical result was considered to be satisfactory in 60 (96.8 %) patients and unsatisfactory with poor result in two (3.2 %) patients. Technical error was thought to be the cause of the poor results. There were no postoperative neural or vascular complications. Conclusion A biplanar crossed pin construct achieved by two Kirschner wires crossed in the coronal and sagittal planes is efficient to stabilize a displaced extension type supracondylar fracture of the humerus in children.
SEOM clinical guidelines for the diagnosis and treatment of gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) 2014
Authors: Garcia-Carbonero R. , JImenez-Fonseca P. , Teulé A. , Barriuso J. , Sevilla I. 
Published: September 2014

Abstract
GEP-NENs are a challenging family of tumors of growing incidence and varied clinical management and behavior. Diagnostic techniques have substantially improved over the past decades and significant advances have been achieved in the understanding of the molecular pathways governing tumor initiation and progression. This has already translated into relevant advances in the clinic. This guideline aims to provide practical recommendations for the diagnosis and treatment of GEP-NENs. Diagnostic workup, histological and staging classifications, and the different available therapeutic approaches, including surgery, liver-directed ablative therapies, peptide receptor radionuclide therapy, and systemic hormonal, cytotoxic or targeted therapy, are briefly discussed in this manuscript. Clinical presentation (performance status, comorbidities, tumor-derived symptoms and hormone syndrome in functioning tumors), histological features [tumor differentiation, proliferation rate (Ki-67), and expression of somatostatin receptors], disease localization and extent, and resectability of primary and metastatic disease, are all key issues that shall be taken into consideration to appropriately tailor therapeutic strategies and surveillance of these patients.
Membranproteinsynthese: Zellfrei geht’s schneller!
Authors: Sachse Rita , Quast Robert B. , Sonnabend Andrei , Stech Marlitt , Kubick Stefan 
Published: September 2014

Abstract
Difficult to express membrane proteins represent an increasing amount of therapeutic molecules. Considerable optimization is often required for downstream applications such as assay development and functional characterization. Cell-free systems emerged as powerful tools for the synthesis of structurally and functionally divergent membrane proteins. Vesicle-based eukaryotic cell-free systems enable co-translational protein translocation and posttranslational modifications. Hence, these systems provide a multitude of options for membrane protein studies.
Brief report: Parent–adolescent child concordance in social norms related to gender equity in marriage – findings from rural India
Authors: Raj Anita , Ghule Mohan , Battala Madhusudana , Dasgupta Anindita , Ritter Julie , Nair Saritha , Saggurti Niranjan , Silverman Jay G. , Balaiah Donta 
Published: September 2014

ABSTRACTThe purpose of this exploratory study was to assess parent–adolescent child concordance on social norms related to gender equity in marriage in rural Maharashtra, India. Survey data on marital norms related to girl's marital age and choice, contraception, and marital violence (MV) were collected from unmarried adolescents (n = 113 girls, 116 boys) and their parents (n = 227 mothers, 203 fathers). Concordance was assessed using a Cohen's unweighted Kappa statistic, with analyses stratified by sex of parent and child. Analyses revealed fair (K = .25–.27) mother‐daughter concordance on girls' right to choose when to marry, contraception use, and acceptability of MV. Father‐son concordance was seen on girls' right to choose when (K = .22, slight) and who (K = .20, fair) to marry and MV acceptability (K = .53, moderate). No opposite sex parent‐child concordance was revealed. Results indicate same but not opposite sex parent‐child concordance on gender equity social norms related to marriage, suggesting same sex transfer of these norms.
Smoking, alcohol consumption, and drug use among adolescents with psychiatric disorders compared with a population based sample
Authors: Mangerud Wenche Langfjord , Bjerkeset Ottar , Holmen Turid Lingaas , Lydersen Stian , Indredavik Marit Sæbø 
Published: September 2014

ABSTRACTThis study investigated frequencies of smoking, alcohol use, and illicit drug use by diagnostic category in 566 adolescent psychiatric patients, comparing this sample with 8173 adolescents from the general population in Norway who completed the Young‐HUNT 3 survey. Frequencies of current alcohol use were high in both samples but were lower among psychiatric patients. Compared with adolescents in the general population, adolescents in the clinical sample had a higher prevalence of current smoking and over four times higher odds of having tried illicit drugs. In the clinical sample, those with mood disorders reported the highest frequencies of smoking, alcohol use, and illicit drug use, whereas those with autism spectrum disorders reported the lowest frequencies. Our results show an increased prevalence of risky health behaviors among adolescents with psychiatric disorders compared with the general population. The awareness of disorder‐specific patterns of smoking and substance use may guide preventive measures.
Expanding the Use of Noninvasive Ventilation During an Epidemic
Authors: Kopić Jasminka , Paradžik Maja Tomić 
Published: September 2014

ABSTRACTNoninvasive ventilation (NIV) is a proved and effective therapeutic option for some patients with respiratory failure. During an epidemic, NIV can free up respirators and other intensive care unit equipment for patients with respiratory insufficiency whose survival depends exclusively on invasive ventilation. Some guidelines have indicated that NIV is potentially hazardous and should not be recommended for use during epidemics, given the perceived potential risk of transmission from aerosolized pathogen dispersion to other patients or medical staff. Conversely, some reports of previous epidemics describe NIV as a very efficient and safe modality of respiratory support, if strict infection control measures are implemented.We discuss NIV use during epidemics and indicate the need for prospective randomized clinical studies on the efficacy of NIV in epidemic conditions to provide important information to the current body of literature. Meanwhile, the use of NIV under strict infection control guidelines should be incorporated into epidemic preparedness planning. (Disaster Med Public Health Preparedness. 2014;8:1-5)