bluuebunny/crossref_metadata_2025_split huggingface dataset:
ABCA1 and nascent HDL biogenesis
Authors: Wang Shuhui , Smith Jonathan D. 
Published: October 2014

AbstractABCA1 mediates the secretion of cellular free cholesterol and phospholipids to an extracellular acceptor, apolipoprotein AI, to form nascent high‐density lipoprotein (HDL). Thus, ABCA1 is a key molecule in cholesterol homeostasis. Functional studies of certain Tangier disease mutations demonstrate that ABCA1 has multiple activities, including plasma membrane remodeling and apoAI binding to cell surface, which participate in nascent HDL biogenesis. Recent advances in our understanding of ABCA1 have demonstrated that ABCA1also mediates unfolding the N terminus of apoAI on the cell surface, followed by lipidation of apoAI and release of nascent HDL. Although ABCA1‐mediated cholesterol efflux to apoAI can occur on the plasma membrane, the role of apoAI retroendocytosis during cholesterol efflux may play a role in macrophage foam cells that store cholesterol esters in cytoplasmic lipid droplets. © 2014 BioFactors, 40(6):547–554, 2014
Evaluation of the immediate vascular stability of lipoprotein lipase‐generated 2‐monoacylglycerol in mice
Authors: Kleberg Karen , Nielsen Louise Lundeman , Stuhr‐Hansen Nicolai , Nielsen John , Hansen Harald Severin 
Published: October 2014

Abstract2‐Monoacylglycerols are gaining increasing interest as signaling lipids, beyond endocannabinoids, for example, as ligands for the receptor GPR119 and as mediators of insulin secretion. In the vascular system, they are formed by the action of lipoprotein lipase (LPL); however, their further disposition is unclear. Assuming similar affinity for uptake and incorporation into tissues of 2‐oleoylglycerol and 2‐oleylglyceryl ether, we have synthesized a 3H‐labeled 2‐ether analog of triolein (labeled in alkyl group) and compared its disposition with 14C‐labeled triolein (labeled in glycerol) 20 min after intravenous coadministration in a ratio of 1:1 to mice. We found that peripheral tissues and the liver in particular are able to take up 2‐monoacylglycerols as seen from 3H uptake. In muscle and adipose tissue, 2‐monoacylglycerols are probably further hydrolyzed as seen by an increased 3H/14C ratio, whereas in the liver and the heart, data suggest that they are also subjected to re‐esterification to triacylglycerol, as seen by an unchanged 3H/14C ratio in the lipid fraction of the tissues. Our findings suggest that LPL‐generated 2‐monoacylglycerol is likely to be stable in the vascular system and thus have a potential to circulate or at least exert effects in tissues where it may be locally produced. © 2014 BioFactors, 40(6):596–602, 2014
Meta-analysis of high- versus low-chloride content in perioperative and critical care fluid resuscitation
Authors: Krajewski M L , Raghunathan K , Paluszkiewicz S M , Schermer C R , Shaw A D 
Published: October 2014

Abstract

Background
The objective of this systematic review and meta-analysis was to assess the relationship between the chloride content of intravenous resuscitation fluids and patient outcomes in the perioperative or intensive care setting.

Methods
Systematic searches were performed of PubMed/MEDLINE, Embase and Cochrane Library (CENTRAL) databases in accordance with PRISMA guidelines. Randomized clinical trials, controlled clinical trials and observational studies were included if they compared outcomes in acutely ill or surgical patients receiving either high-chloride (ion concentration greater than 111 mmol/l up to and including 154 mmol/l) or lower-chloride (concentration 111 mmol/l or less) crystalloids for resuscitation. Endpoints examined were mortality, measures of kidney function, serum chloride, hyperchloraemia/metabolic acidosis, blood transfusion volume, mechanical ventilation time, and length of hospital and intensive care unit stay. Risk ratios (RRs), mean differences (MDs) or standardized mean differences (SMDs) and confidence intervals were calculated using fixed-effect modelling.

Results
The search identified 21 studies involving 6253 patients. High-chloride fluids did not affect mortality but were associated with a significantly higher risk of acute kidney injury (RR 1·64, 95 per cent c.i. 1·27 to 2·13; P &lt; 0·001) and hyperchloraemia/metabolic acidosis (RR 2·87, 1·95 to 4·21; P &lt; 0·001). High-chloride fluids were also associated with greater serum chloride (MD 3·70 (95 per cent c.i. 3·36 to 4·04) mmol/l; P &lt; 0·001), blood transfusion volume (SMD 0·35, 0·07 to 0·63; P = 0·014) and mechanical ventilation time (SMD 0·15, 0·08 to 0·23; P &lt; 0·001). Sensitivity analyses excluding heavily weighted studies resulted in non-statistically significant effects for acute kidney injury and mechanical ventilation time.

Conclusion
A weak but significant association between higher chloride content fluids and unfavourable outcomes was found, but mortality was unaffected by chloride content.
Combined derivatization and high‐performance liquid chromatography with fluorescence and ultraviolet detection for simultaneous analysis of octreotide and gabexate mesylate metabolite in human pancreatic juice samples
Authors: Carlucci Giuseppe , Selvaggi Federico , Sulpizio Sara , Bassi Claudio , Carlucci Maura , Cotellese Roberto , Ferrone Vincenzo , Innocenti Paolo , Locatelli Marcello 
Published: October 2014

AbstractA simple and sensitive method based on the combination of derivatization and high‐performance liquid chromatography with ultraviolet and fluorimetric detection was developed for the simultaneous determination of octreotide and gabexate mesylate metabolite in human pancreatic juice samples. Parameters of the derivatization procedure affecting extraction efficiency were optimized. The developed method was validated according to the International Conference on Harmonization guidelines. The calibration curves were linear over a range of 0.1–15 µg/mL for octreotide and 0.20‐15 µg/mL for gabexate mesylate metabolite. Derivatized products of octreotide and gabexate mesylate metabolite were separated on a Luna C18 column (4.6 × 250 mm; 5 µm particle size) using a gradient with a run time of 36 min, without further purification. The limits of detection were 0.025 and 0.05, respectively, for octreotide and gabexate mesylate metabolite. This paper reports the validation of a quantitative high performance liquid chromatography–photodiode array–fluorescence (HPLC‐PDA‐FL) method for the simultaneous analysis of octreotide and gabexate mesylate metabolite in pancreatic juice by protein precipitation using zinc sulfate–methanol–acetonitrile containing the derivatizing reagent, 4‐fluoro‐7‐nitro‐[2,1,3]‐benzoxadiazole (NBD‐F). Derivatized products of octreotide and gabexate mesylate metabolite were separated on a Luna C18 column (4.6 × 250 mm; 5 µm particle size) using a gradient with a run time of 36 min, without further purification. The method was validated over the concentration ranges 0.1–15 and 0.2–15 µg/mL for octreotide and gabexate mesylate metabolite, respectively, in human pancreatic juice. Biphalin and methyl‐p‐hydroxybenzoate were used as the internal standards. This method was successfully utilized to support clinical studies in humans. The results from assay validations show that the method is selective, sensitive and robust. The limit of quantification of the method was 0.1 µg/mL for octreotide and 0.2 µg/mL for gabexate mesylate metabolite, and matrix matched standard curves showed a good linearity up to 15 µg/mL. In the entire analytical range the intra‐ and inter‐day precision (RSD%) values were respectively ≤5.9% and ≤3.1% for octreotide and ≤2.0% and ≤3.9% for gabexate mesylate metabolite. For both analytes the intra‐ and inter‐day accuracy (bias) values ranged respectively from −6.8 to –2.5% and from −4.6 to −5.7%. This method utilizes derivatization with NBD‐F and provides adequate sensitivity for both drugs. Copyright © 2014 John Wiley & Sons, Ltd.
Establishment of new predictive markers for distant recurrence of colorectal cancer using lectin microarray analysis
Authors: Nakajima Kentaro , Inomata Masafumi , Iha Hidekatsu , Hiratsuka Takahiro , Etoh Tsuyoshi , Shiraishi Norio , Kashima Kenji , Kitano Seigo 
Published: October 2014

AbstractWe evaluated the clinical benefits of novel predictive markers for distant recurrence with colorectal cancer using lectin microarray analysis of cell surface glycan modifications. Glycoproteins were extracted from formalin‐fixed, paraffin‐embedded tumor specimens and normal epithelium from 53 consecutive curatively resected stage I–III colorectal cancer cases and then subjected to lectin microarray to obtain lectin–glycan interaction (LGI) values. In addition, clinicopathological factors associated with distant recurrence were identified. LGI values that were associated with distant recurrence were validated with an additional 55 curatively resected stage II colorectal cancer cases. LGI values for Agaricus bisporus (ABA) lectin, prominent in cancer tissues, were statistically associated with distant recurrence. ABA lectin staining exhibited strikingly intense signals in the cytoplasm and apical surfaces of cancer cells, while weak staining was observed in the supranuclear regions of normal epithelium. This ABA tumor/normal LGI ratio may be a new predictive biomarker for distant recurrence of curatively resected colorectal cancer.
Experimental and Computational Studies of the Molybdenum‐Flanking Arene Interaction in Quadruply Bonded Dimolybdenum Complexes with Terphenyl Ligands
Authors: Carrasco Mario , Mendoza Irene , Álvarez Eleuterio , Grirrane Abdessamad , Maya Celia , Peloso Riccardo , Rodríguez Amor , Falceto Andrés , Álvarez Santiago , Carmona Ernesto 
Published: October 2014

AbstractTo clarify the nature of the MoCarene interaction in terphenyl complexes with quadruple MoMo bonds, ether adducts of composition [Mo2(Ar′)(I)(O2CR)2(OEt2)] have been prepared and characterized (Ar′=ArXyl2, R=Me; Ar′=ArMes2, R=Me; Ar′=ArXyl2, R=CF3) (Mes=mesityl; Xyl=2,6‐Me2C6H3, from now on xylyl) and their reactivity toward different neutral Lewis bases investigated. PMe3, P(OMe)3 and PiPr3 were chosen as P‐donors and the reactivity studies complemented with the use of the C‐donors CNXyl and CN2C2Me4 (1,3,4,5‐tetramethylimidazol‐2‐ylidene). New compounds of general formula [Mo2(Ar′)(I)(O2CR)2(L)] were obtained, except for the imidazol‐2‐ylidene ligand that yielded a salt‐like compound of composition [Mo2(ArXyl2)(O2CMe)2(CN2C2Me4)2]I. The MoCarene interaction in these complexes has been analyzed with the aid of X‐ray data and computational studies. This interaction compensates the coordinative and electronic unsaturation of one of the Mo atoms in the above complexes, but it seems to be weak in terms of sharing of electron density between the Mo and Carene atoms and appears to have no appreciable effect in the length of the MoMo, MoX, and MoL bonds present in these molecules.
Stable and Low‐Cost Mesoscopic CH3NH3PbI2Br Perovskite Solar Cells by using a Thin Poly(3‐hexylthiophene) Layer as a Hole Transporter
Authors: Zhang Meng , Lyu Miaoqiang , Yu Hua , Yun Jung‐Ho , Wang Qiong , Wang Lianzhou 
Published: October 2014

AbstractMesoscopic perovskite solar cells using stable CH3NH3PbI2Br as a light absorber and low‐cost poly(3‐hexylthiophene) (P3HT) as hole‐transporting layer were fabricated, and a power conversion efficiency of 6.64 % was achieved. The partial substitution of iodine with bromine in the perovskite led to remarkably prolonged charge carrier lifetime. Meanwhile, the replacement of conventional thick spiro‐MeOTAD layer with a thin P3HT layer has significantly reduced the fabrication cost. The solar cells retained their photovoltaic performance well when they were exposed to air without any encapsulation, presenting a favorable stability. The combination of CH3NH3PbI2Br and P3HT may render a practical and cost‐effective solid‐state photovoltaic system. The superior stability of CH3NH3PbI2Br is also promising for other photoconversion applications.
Electronic Properties of Mono‐Substituted Tetraferrocenyl Porphyrins in Solution and on a Gold Surface: Assessment of the Influencing Factors for Photoelectrochemical Applications
Authors: Vecchi Andrea , Erickson Nathan R. , Sabin Jared R. , Floris Barbara , Conte Valeria , Venanzi Mariano , Galloni Pierluca , Nemykin Victor N. 
Published: October 2014

AbstractTwo unsymmetric meso‐tetraferrocenyl‐containing porphyrins of general formula Fc3(FcCOR)Por (Fc=ferrocenyl, R=CH3 or (CH2)5Br, Por=porphyrin) were prepared and characterized by a variety of spectroscopic methods, whereas their redox properties were investigated using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) approaches. The mixed‐valence [Fc3(FcCOR)Por]n+ (n=1,3) were investigated using spectroelectrochemical as well as chemical oxidation methods and corroborated with density functional theory (DFT) calculations. Inter‐valence charge‐transfer (IVCT) transitions in [Fc3(FcCOR)Por]+ were analyzed, and the resulting data matched closely previously reported complexes and were assigned as Robin–Day class II mixed‐valence compounds. Self‐assembled monolayers (SAMs) of a thioacetyl derivative (Fc3(FcCO(CH2)5SCOCH3)Por) were also prepared and characterized. Photoelectrochemical properties of SAMs in different electrolyte systems were investigated by electrochemical techniques and photocurrent generation experiments, showing that the choice of electrolyte is critical for efficiency of redox‐active SAMs.
Novel Composite Plastics Containing Silver(I) Acylpyrazolonato Additives Display Potent Antimicrobial Activity by Contact
Authors: Marchetti Fabio , Palmucci Jessica , Pettinari Claudio , Pettinari Riccardo , Condello Francesca , Ferraro Stefano , Marangoni Mirko , Crispini Alessandra , Scuri Stefania , Grappasonni Iolanda , Cocchioni Mario , Nabissi Massimo , Chierotti Michele R. , Gobetto Roberto 
Published: October 2014

AbstractNew silver(I) acylpyrazolonato derivatives displaying a mononuclear, polynuclear, or ionic nature, as a function of the ancillary azole ligands used in the synthesis, have been fully characterized by thermal analysis, solution NMR spectroscopy, solid‐state IR and NMR spectroscopies, and X‐ray diffraction techniques. These derivatives have been embedded in polyethylene (PE) matrix, and the antimicrobial activity of the composite materials has been tested against three bacterial strains (E. coli, P. aeruginosa, and S. aureus): Most of the composites show antimicrobial action comparable to PE embedded with AgNO3. Tests by contact and release tests for specific migration of silver from PE composites clearly indicate that, at least in the case of the PE, for composites containing polynuclear silver(I) additives, the antimicrobial action is exerted by contact, without release of silver ions. Moreover, PE composites can be re‐used several times, displaying the same antimicrobial activity. Membrane permeabilization studies and induced reactive oxygen species (ROS) generation tests confirm the disorganization of bacterial cell membranes. The cytotoxic effect, evaluated in CD34+ cells by MTT (3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐diphenyltetrazoliumbromide) and CFU (colony forming units) assays, indicates that the PE composites do not induce cytotoxicity in human cells. Studies of ecotoxicity, based on the test of Daphnia magna, confirm tolerability of the PE composites by higher organisms and exclude the release of Ag+ ions in sufficient amounts to affect water environment.
A Multi‐Addressable Switch Based on the Dimethyldihydropyrene Photochrome with Remarkable Proton‐Triggered Photo‐opening Efficiency
Authors: Roldan Diego , Cobo Saioa , Lafolet Frédéric , Vilà Neus , Bochot Constance , Bucher Christophe , Saint‐Aman Eric , Boggio‐Pasqua Martial , Garavelli Marco , Royal Guy 
Published: October 2014

AbstractA series of photochromic derivatives based on the trans‐10b,10c‐dimethyl‐10b,10c‐dihydropyrene (DHP, “closed form”) skeleton has been synthesized and their photoisomerization leading to the corresponding cyclophanediene (CPD, “open form”) isomers has been investigated by UV/Vis and 1H NMR spectroscopies. Substitution of the DHP core with electron‐withdrawing pyridinium groups was found to have major effects on the photoisomerization efficiency, the most remarkable examples being to enhance the quantum yield of the opening reaction and to allow fast and quantitative conversions at much lower radiant energies. This effect was rationalized by theoretical calculations. We also show that the reverse reaction, that is, going from the open form to the closed form, can be electrochemically triggered by oxidation of the CPD unit and that the photo‐opening properties of pyridine‐substituted DHPs can be efficiently tuned by protonation, the system behaving as a multi‐addressable molecular switch. These multi‐addressable photochromes show promise for the development of responsive materials.
Self‐Assembly of N‐Heterocyclic Derivatives of Divalent Germanium, Tin, and Lead
Authors: Zabula Alexander V. , Rogachev Andrey Yu. , West Robert 
Published: October 2014

AbstractA new class of exceptionally stable asymmetric N‐heterocyclic germylenes, stannylenes, and plumbylenes has been successfully isolated and characterized by single‐crystal X‐ray diffraction analysis and multinuclear NMR spectroscopy. Their stability results from tetrameric supramolecular aggregation through strong intermolecular Npy→EII (E=Ge, Sn, Pb) interactions involving the nitrogen atom of a neighboring pyridine moiety. The electronic structures and stabilities of the prepared divalent derivatives of Ge, Sn, and Pb in monomeric and aggregated forms are discussed based on theoretical investigations.
Deuterium MAS NMR Studies of Dynamics on Multiple Timescales: Histidine and Oxalic Acid
Authors: Chan‐Huot Monique , Wimperis Stephen , Gervais Christel , Bodenhausen Geoffrey , Duma Luminita 
Published: October 2014

AbstractDeuterium (2H) magic‐angle spinning (MAS) nuclear magnetic resonance is applied to monitor the dynamics of the exchanging labile deuterons of polycrystalline L‐histidine hydrochloride monohydrate‐d7 and α‐oxalic acid dihydrate‐d6. Direct experimental evidence of fast dynamics is obtained from T1Z and T1Q measurements. Further motional information is extracted from two‐dimensional single‐quantum (SQ) and double‐quantum (DQ) MAS spectra. Differences between the SQ and DQ linewidths clearly indicate the presence of motions on intermediate timescales for the carboxylic moiety and the D2O in α‐oxalic acid dihydrate, and for the amine group and the D2O in L‐histidine hydrochloride monohydrate. Comparison of the relaxation rate constants of Zeeman and quadrupolar order with the relaxation rate constants of the DQ coherences suggests the co‐existence of fast and slow motional processes.
Anticorrelation between Surface and Subsurface Point Defects and the Impact on the Redox Chemistry of TiO2(110)
Authors: Yoon Yeohoon , Du Yingge , Garcia Juan C. , Zhu Zihua , Wang Zhi‐Tao , Petrik Nikolay G. , Kimmel Gregory A. , Dohnalek Zdenek , Henderson Michael A. , Rousseau Roger , Deskins N. Aaron , Lyubinetsky Igor 
Published: October 2014

AbstractBy using a combination of scanning tunneling microscopy (STM), density functional theory (DFT), and secondary‐ion mass spectroscopy (SIMS), we explored the interplay and relative impact of surface versus subsurface defects on the surface chemistry of rutile TiO2. STM results show that surface O vacancies (VO) are virtually absent in the vicinity of positively charged subsurface point defects. This observation is consistent with DFT calculations of the impact of subsurface defect proximity on VO formation energy. To monitor the influence of such lateral anticorrelation on surface redox chemistry, a test reaction of the dissociative adsorption of O2 was employed and was observed to be suppressed around them. DFT results attribute this to a perceived absence of intrinsic (Ti), and likely extrinsic interstitials in the nearest subsurface layer beneath inhibited areas. We also postulate that the entire nearest subsurface region could be devoid of any charged point defects, whereas prevalent surface defects (VO) are largely responsible for mediation of the redox chemistry at the reduced TiO2(110).
Spur reduction in frequency synthesizer with an array of switched capacitors
Authors: Mandal Debashis , Mandal Pradip , Bhattacharyya Tarun Kanti 
Published: October 2014

SummaryThis paper proposes a new spur reducing architecture of phase‐locked loop‐based frequency synthesizer. With an array of switched capacitors and a delay locked loop, the charge coming from its charge pump is evenly transferred to its loop filter at a fixed number of equi‐spaced time intervals. It results in the reduction of fundamental and higher‐order harmonics of the reference spur. The proposed architecture has been designed and fabricated in 180 nm CMOS technology. Randomization scheme has also been incorporated in the proposed architecture to reduce the effect of implementation mismatch on the output spur. Measured result shows about 5.47 dB reduction of fundamental spur compared to that of the conventional architecture with almost no degradation of noise performance. Copyright © 2014 John Wiley & Sons, Ltd.
A fast‐corrected all‐digital DCC with synchronous input clock
Authors: Kao Shao‐Ku , Hsueh Sheng‐Hung 
Published: October 2014

SummaryThis paper presents a fast‐corrected all‐digital duty‐cycle corrector (DCC) with synchronous input clock. The proposed DCC has many features, including fast locking in 4 cycles, wide range correction, and synchronous 50% duty‐cycle clock with an input clock. The circuit can operate from 500 to 900 MHz and corrects a wide range of input duty cycle ranging from 25 to 75%. The duty‐cycle error of the output clock is between −2.4 and 2.7%. The largest static phase error between the input and output clock is −44 ps at 900 MHz. The RMS and peak‐to‐peak jitters are 1.9 and 14.7 ps at 900 MHz, respectively. The proposed DCC is implemented in a 0.18‐µm complementary metal oxide semiconductor process. The proposed DCC occupies an area of 0.05 mm2 and dissipates 23 mW with 1.8‐V supply voltage at 900 MHz. Copyright © 2014 John Wiley & Sons, Ltd.
Selective social buffering of behavioral and endocrine responses and Fos induction in the prelimbic cortex of infants exposed to a novel environment
Authors: Hennessy Michael B. , Schiml Patricia A. , Willen Regina , Watanasriyakul Withayapon , Johnson Joshua , Garrett Teresa 
Published: October 2014

ABSTRACTIn mammals, the presence of the mother can reduce or “buffer” stress responses of her young in threatening conditions. We compared the effect of the mother, a familiar littermate, and an unfamiliar adult male on three classes of response shown by guinea pig pups in a novel environment: short latency active behaviors, particularly vocalizing; slower developing passive behaviors that appear mediated by inflammatory mechanisms; and hypothalamic‐pituitary‐adrenal activity. We also examined Fos induction in the prelimbic cortex, a region hypothesized to mediate buffering effects. Only the mother significantly suppressed all classes of behavior. The greatest selectivity was observed for passive behavioral responses. Contrary to expectations, the adult male reduced plasma cortisol levels of pups as effectively as did the mother. The presence of the male also resulted in increased Fos induction in the prelimbic cortex and high levels of social interaction. Maternal buffering was not associated with prelimbic activity. These results confirm the ability of the mother to reduce active behavioral and HPA responses and suggest a specific maternal buffering effect on the later developing passive behavioral responses. The findings also demonstrate an unexpected ability of adult males to reduce HPA responses and raise the possibility that different social partners buffer HPA activity through different underlying processes. © 2014 Wiley Periodicals, Inc. Dev Psychobiol 57: 50–62, 2015.