bluuebunny/crossref_metadata_2025_split huggingface dataset:
HISTIDINE‐RICH PROTEIN AS A POSSIBLE ORIGIN OF FREE AMINO ACIDS OF STRATUM CORNEUM
Authors: Horii Izumi , Kawasaki Kiyoshi , Koyama Junichi , Nakayama Yasuhisa , Nakajima Keisuke , Okazaki Kuninobu , Seiji Makoto 
Published: April 2015

ABSTRACTPulse‐chase labeling experiments with 3H‐histidine and 3H‐arginine were performed in hairless mice to investigate the origin of free amino acids in the stratum corneum. Time‐course labeling of epidermal proteins was examined by sodium dodecyl sulfate‐polyacryl amide gel electrophoresis (SDS‐PAGE). The radioactivity was also measured in the following three epidermal fractions; 0.1 N HClO4 soluble‐ethanol soluble fraction (Fr. I), 0.1 N HClO4 soluble‐ethanol insoluble fraction (Fr. II, histidine‐rich protein fraction) and 0.1 N HClO4 insoluble‐8M urea soluble fraction (Fr. III).Labeled amino acids were rapidly incorporated into Fr. III, especially into a particular protein which showed little mobility on SDS‐PAGE. As the radioactivity of this protein decreased, an increase in radioactivity was observed in a 32,000 MW histidine‐rich protein, the only major protein in Fr. II. The radioactivity of 32,000 MW protein then fell, accompanied by an increase in radioactivity of Fr. I. The major radioactive substances in Fr. I were identified as 3H‐histidine and 3H‐urocanic acid after 3H‐histidine injection and as 3H‐arginine after 3H‐arginine injection. The amino acid composition of the histidine‐rich protein was very similar to the composition of free amino acids of the stratum corneum, in which amino acid metabolites were considered as their precursor amino acids.These results suggested that the free amino acids of the stratum corneum might be a final product of the degradation of histidine‐rich protein.
SYSTEMIC EFFECTS OF 0.064% BETAMETHASONE DIPROPIONATE OINTMENT AND CREAM BY SIMPLE APPLICATION
Authors: Aso Miki , Shimao Shuhei 
Published: April 2015

ABSTRACTForty‐six patients with various skin diseases applied 0.064% betamethasone dipropionate (BD) ointment and cream simply for 3 to 6 weeks, and serum cortisol, blood sugar, and peripherocirculatory eosinophyll counts were measured.Serum cortisol was almost unaffected by the application of BD ointment or cream at the rate of 10 g a day. After using between 10 and 20 g a day a reducing trend in cortisol levels was observed with BD ointment, but not with BD cream. When the application was increased to 20 g a day or more, a marked reduction was noted. This reduction was temporary and the serum cortisol returned to the normal level in no more than one week after the treatment. Peripherocirculatory eosinophyle counts decreased with application of even less than 10 g per day, and then markedly decreased further as the dose increased. Blood sugar was not increased by BD ointment or cream.Topical corticosteroid treatment is generally used by outpatients who usually receive less than 10 g of medication per day. Thus, it was presumed that, for outpatients, simple application of BD ointment or cream should not cause any systemic effects, and that, even if there were effects, they would only be slight, as far as suppression of adrenocortical functions was concerned.
A CASE OF THE HYPEREOSINOPHILIC SYNDROME COMPLICATED BY MALIGNANT LYMPHOMA
Authors: Sato Akihiko , Kobayashi Kazuo , Okabe Hitoshi , Fukuda Sembun 
Published: April 2015

ABSTRACTA case of hypereosinophilic syndrome complicated by malignant lymphoma is reported, including autopsy results. The malignant lymphoma was pleomorphic and characterized clinically and histologically by its unusual localization in the brain. The disturbed immune system of the patient, who had been immunologically compromised by immunosuppression therapy consisting of predonisolone and azathioprine, seemed relevant to the pathogenesis of this malignant lymphoma.
SQUAMOUS CELL CARCINOMA COMPLICATING CHRONIC DISCOID LUPUS ERYTHEMATOSUS (CDLE)
Authors: Sehgal V.N. , Reddy B.S.N. , Koranne R.V. , Sharma A.K. , Jain R.K. 
Published: April 2015

ABSTRACTOccurrence of squamous cell carcinoma with some unusual clinical features is described in two female patients.
NODULAR FASCIITIS UNDER A SPLINT
Authors: Kikuchi Ichiro , Kuroki Yasumasa 
Published: April 2015

ABSTRACTA 61‐year‐old man developed nodular fasciitis on the left forearm at the center of a splint used for the immobilization of a skin graft for the treatment of squamous cell carcinoma. It was first noted by the patient 30 days after the operation and 22 days after the removal of the splint.
PENETRATION OF FRAGRANCE COMPOUNDS THROUGH HUMAN EPIDERMIS
Authors: Jimbo Yuko 
Published: April 2015

ABSTRACTPenetration through excised human epidermis of six phenolic, four benzyl, two salicylate and six cinnamic compounds was examined. A considerable difference in penetration of the compounds tested was observed. Benzyl alcohol penetrated most rapidly. Solubility in water and octanol of each compound was studied. All compounds were freely soluble in octanol. A high correlation between water solubility and percent penetration through human epidermis of compounds was observed. The percent penetration of compounds was not related to allergenic potency.
EFFECTS OF KC‐400 (POLYCHLORINATED BIPHENYLS) ON PORPHYRIN METABOLISM
Authors: Honda Tetsuzo , Nonaka Shigeo , Murayama Fumio , Ohgami Taro , Shimoyama Tokio , Yoshida Hikotaro 
Published: April 2015

ABSTRACTTo investigate the influence of polychlorinated biphenyls (PCB) on porphyrin metabolism, Wistar rats were orally administered KC‐400. The mean value of liver/body weight ratio in normal rats was 3.86%, with a range of 3.44% to 5.22%. Their mean blood protoporphyrin level was 23.3 μg/dl packed cell volume (p.c.v.), with a range of 11.8 to 64.4 μg/dl p.c.v., and their mean liver protoporphyrin level was 0.17 μg/gm wet weight, with a range of 0.03 to 0.40 μg/gm wet weight.A feed containing KC‐400 (140–160 ppm) was given for 2 to 20 weeks to 20 rats. The mean value of liver/body weight ratio was 5.00%, with a range of 3.80% to 6.16%. The mean erythrocyte protoporphyrin level was 47.4 μg/dl p.c.v., with a range of 19.7 to 83.2 μg/dl p.c.v., and the mean liver protoporphyrin level was 0.36 μg/gm wet weight, with a range of 0.04 to 0.67 μg/gm wet weight. The liver coproporphyrin level showed abnormalities in 2 of 20 rats fed the feed containing KC‐400, with values of 0.71 and 9.89 μg/gm wet weight, respectively. After administration of KC‐400 feed for 43 weeks, the mean value of blood coproporphyrin level rose to 32.1 μg/dl p.c.v., with a range of 11.4 to 54.6 μg/dl p.c.v. The mean erythrocyte protoporphyrin level was 74.3 μg/dl p.c.v., with a range of 49.5 to 102.1 μg/dl p.c.v. The mean liver coproporphyrin level was elevated to 5.15 μg/gm wet weight, with a range of 0.45 to 9.48 μg/gm wet weight, and the mean liver protoporphyrin level was 0.65 μg/gm wet weight, with a range of 0.43 to 9.88 μg/gm wet weight. Thus, there was an increase in both liver porphyrin and coproporphyrin following KC‐400 feeding. Liver cells in the rats with abnormal porphyrin levels exhibited a red fluorescence. From these results, we can deduce that KC‐400 may induce abnormalities in porphyrin metabolism, when it is fed orally for a long period of time. These abnormalities in porphyrin metabolism are limited to the liver only.
STASIS DERMATITIS IN A PATIENT WITH NOONAN'S SYNDROME
Authors: Tsuzuki Chiharu , Hidano Akira 
Published: April 2015

ABSTRACTA typical 20 year old case of Noonan's syndrome is reported. Skin symptoms included curly hair, low hairline, ptosis, and increased elasticity over the whole skin. The patient also had a stasis dermatitis which had begun at the age of 18.
STUDY ON THE SUPPRESSOR PHENOMENON INDUCED IN CONTACT HYPERSENSITIVITY REACTION
Authors: Kotake Kimiko , Kanagawa Hisami , Semma Masanori , Sagami Seichiro 
Published: April 2015

ABSTRACTFrom the present experiments in which sensitization was induced by painting contactants on the same skin of the same mouse over time, the following findings were observed.

When mice were sensitized first with PCl, then with DNFB, and finally with OX, the animals showed a normal contact hypersensitivity response to PCl, but abnormal responses to both DNFB and OX, suggesting some suppressor phenomenon.
This suppression was shown to be partly due to hapten (DNFB)‐specific suppressor cells, and it was suggested that other suppressor cells different from suppressor T cells in nature might be involved.
It was considered that induction of suppressor cells might be due to depressed function of the epidermal Langerhans cell affected by potent sensitizers as well as by unknown mechanisms.
LABORATORY STUDY OF COLLAGEN WOUND DRESSING (CAS)
Authors: Takeda Ueto , Izawa Masanori , Koeda Takemi , Shibata Uichi 
Published: April 2015

ABSTRACTIn order to determine whether collagen wound dressing (CAS) has any immunogenicity, we carried out various immunological tests with Japanese albino rabbits and BALB/c mice. CAS extract produced no antibodies while atelocollagen, the source material of CAS, has less antigenicity than acid soluble collagen, a tropocollagen. Moreover, the antigenicity of CAS was much lower than that of lyophilized porcine skin which is much used in clinical practice. It is assumed that CAS would be immunologically safe as a wound dressing.
PLATELET FUNCTION IN BEHÇET'S DISEASE
Authors: Haim S. , Brook J.G. , Gilhar A. , Friedman‐Birnbaum R. , Markel A. , Aviram M. , Marmur A. , Youdin M. 
Published: April 2015

ABSTRACTThe coagulopathy observed in Behçet's disease has defied explanation. No pathology in concentration or function of coagulation parameters has been demonstrated. We have studied platelet adhesion function in 13 patients with established Behçet's disease, 5 with venous thrombosis and 8 with no involvement of veins, as well as in 30 age and sex matched healthy controls. Platelets derived from 10 of the 13 Behçet's patients exhibited significantly increased adhesion as compared to the controls (p<0.01). The platelets of 11 of the 13 tested patients showed significantly increased aggregation in response to adenosine diphosphate (ADP) and epinephrine incomparison to those from controls (p<0.01).However, no correlation between disturbed platelet function and the clinical occurrence of the thrombotic phenomenon was noted. It is suggested that the thrombotic tendency may be related to platelet abnormality while the generation of the actual process is dependent on other factors which await elucidation.
MALIGNANT SKIN TUMORS AMONG DERMATOLOGY PATIENTS IN UNIVERSITY HOSPITALS OF JAPAN
Authors: Tada Masanori , Miki Yoshiharu 
Published: April 2015

ABSTRACTA total of 829 patients with squamous cell carcinoma (SCC), 942 patients with basal cell carcinoma (BCC) and 256 patients with malignant melanoma (MM) was encountered among 1,033,678 new dermatology patients in 44 of 46 university hospitals of Japan between 1971 and 1975. The incidences of skin cancers (SCC and BCC) and of MM among new dermatology patients were 0.171% and 0.025% respectively. The incidences had increased 2 to 2.5 times over those of the previous survey between 1956 and 1960.Skin cancers were most frequently seen among those over 60 years of age and on exposed sites. Burn scars, chronic radiodermatitis, traumatic scars and xeroderma pigmentosum were common preceding diseases in skin cancers, although the majority of the cancers were without apparent preceding diseases. The incidence of skin cancers on exposed sites without preceding diseases showed a statistically significant exponential correlation with the decreasing degrees of the Northern Latitude (NL) of the university locations. The incidence of MM was most common on the extremities and showed no such geographic correlation.
FAMILIAL HYPERKERATOSIS LENTICULARIS PERSTANS
Authors: Ishibashi Akira , Tsuboi Ryoji , Fujita Keiichi 
Published: April 2015

ABSTRACTA 58‐year‐old male patient with familial hyperkeratosis lenticularis perstans (HLP) was reported. Six members of his family have died of cancers of the stomach, liver, and colon. There may be some relationship between genes participating in oncogenesis and HLP genes. Histologically, serial sections of several papules revealed no marked thinning of the epidermis nor severe cell infiltration in the upper dermis, suggesting that the cell infiltration in the upper dermis was secondary.
TRANSFER OF A DRUG‐INDUCED GENERALIZED RASH IN GUINEA PIGS
Authors: Kitamura Kazuko 
Published: April 2015

ABSTRUCTPassive transfer of experimentally induced drug‐rash (GR) was studied in JY‐1 strain guinea pigs. Sulbenicillin (SBPC)‐induced GR was transferred into four of ten recipients by SBPC immunized cells. CET‐induced GR was transferred into twelve of seventeen recipients by CET‐immunized cells and accidentally transferred into two of fifteen recipients by CET‐immunized serum. However, CET‐induced GR was not successfully transferred by SBPC‐immunized cells, complete Freund's adjuvant (CFA)‐immunized cells, frozen and thawed materials, or sonicated cells of CET‐immunized cells. Anti‐CET antibody of CET‐immune serum was not detected by passive hemagglutination assay.CET‐immunized cells were separated into adherent and nonadherent fractions (Ad and Non‐Ad) in laboratory dishes coated with anti‐guinea pig γ‐globulin. CET‐induced GR was not transferred by Ad, but was transferred into eight of twenty‐two recipients by Non‐Ad. Only 4.6% of Non‐Ad cells were immunoglobulin bearing. Transferred GR appeared about 19–24 hr after GR‐elicitation. Erythema was paler than that of GR in actively sensitized guinea pigs.Microscopic examination of the skin taken about 24 hr after the cell transfer with systemic challenge revealed edema, dilation and compaction of capillaries, and diffuse lymphoid cell infiltration with a few eosinophils and basophils in the upper dermis. After about 48–72 hr, basophils were not confirmed as in actively sensitized animals. The count of peripheral leukocytes increased until 24 hr after GR elicitation in both recipients of immunized cells and immunized serum regardless of the severity of the GR reaction. It is likely that, in the transfer of drug‐induced GR, immune cells (probably primed T cells) which do not bear immunoglobulin have a significant role in GR, but serum factors also are involved, at least in some aspects of GR.
MONONUCLEAR CELL VERSUS MONOCYTE CHEMOTAXIS IN PSORIASIS
Authors: Tosca Androniki , Hatzis John , Panteleos Demeter , Varelzidis Anthony 
Published: April 2015

ABSTRACTChemotaxis under agarose of psoriatic mixed mononuclear cells (MNs) and pure monocytes against Zymosan treated sera (ZAS), fresh psoriatic serum, and fresh normal serum was studied and compared with that of normal cells. Purification of monocytes was achieved on microexudate coated BHK flasks. Psoriatic MNs were found to be more chemotactic against psoriatic serum (P.S.) than normal MNs against normal serum (N.S.). Psoriatic cells were chemotactically more active than normal ones against psoriatic and normal serum but not against ZAS. Mixed mononuclear cells showed definitely increased chemotaxis over that of pure monocytes. When culture supernatants with lymphocyte derived factor (LDCF) activity were mixed with normal pure monocytes, the chemotactic activity of the monocytes recovered. It is, therefore, proposed that the LDCF from in vivo stimulated psoriatic lymphocytes by antigenic or mitogenic substances of psoriatic plaque might be an essential factor for the increased chemotaxis of mixed psoriatic MNs compared to pure monocytes. This hypothesis also explains the increased chemoattracting capacity of psoriatic serum for psoriatic and normal MNs as due to the in vivo release of lymphokine.
DRUG‐INDUCED LYMPHOCYTE PROLIFERATION IN THE EXPERIMENTAL SULBENICILLIN (SBPC) RASH OF GUINEA PIGS AND THE ENHANCEMENT EFFECTS OF INTERLEUKIN 2 (IL2)
Authors: Ikezawa Zenro , Aihara Michiko , Nagai Ryukichi 
Published: April 2015

ABSTRACTThe effects of interleukin 2 (IL2) on drug‐specific lymphocyte proliferative responses and their suppression by elicitation of sulbenicillin (SBPC) rash and by induction of SBPC tolerance were studied. GR‐LC and TO‐LC, the draining lymph node cells taken from animals with SBPC generalized rash (GR) and SBPC tolerance (TO), respectively, were incubated with IL2 rich medium (IL2RM) overnight, then washed, and tested for antigen‐specific response in a standard 3‐day proliferation assay. The GR‐LC and TO‐LC showed low responsiveness or unresponsiveness to specific drug before overnight incubation with IL2LM, but recovered a distinct responsiveness after overnight incubation with IL2LM. This result is encouraging for the establishment of the drug‐specific lymphocyte proliferation test for determination of the causative drug in patients with delayed type hypersensitivity drug eruptions.
DEMONSTRATION OF EOSINOPHIL CHEMOTACTIC FACTOR IN THE BLISTER FLUID OF PATIENT WITH INCONTINENTIA PIGMENTI
Authors: Tsuda Shingo , Higuchi Mitsunari , Ichiki Mikio , Sasai Yoichiro 
Published: April 2015

ABSTRACTA case of incontinentia pigmenti associated with tissue and peripheral blood eosinophilia was reported. Eosinophil chemotactic factor (ECF) with low molecular weight was detected in the blister fluid at 17 days after birth by an in vitro chemotactic assay. ECF showed two different molecular weights; one was approximately 1355 daltons and the other much smaller. A similar factor was also detected in the content of a suction blister in clinically uninvolved skin at 2 months after birth. Investigations of ECF generation appears to be important in understanding of this disease.
CONTACT SENSITIVITY IN RATS INDUCED BY TOLYLENE DIISOCYANATE (TDI)
Authors: Tanaka Ken‐Ichi , Nagaya Yoshiko , Marui Shogo , Okamoto Yutaka , Hanada Satoshi 
Published: April 2015

ABSTRACTA model of contact sensitivity (CS) based upon ear swelling has been developed in rats, which are regarded as low responders to common contact sensitizers. For sensitization, a 1% solution of tolylene diisocyanate (TDI) in ethyl acetate was applied to male F344 rats in three different ways; the solution was applied to the shaved backs (1st group), to the unshaved tails (2nd group), and to the tails and then, seven days later, to the backs (3rd group). All the rats were ear‐challenged seven days after the above treatments. The most remarkable ear swelling was observed in the 3rd group, the rats sensitized by the double application method. Delayed onset of the ear response together with histology of the swollen ear showed that the response was characteristic of delayed‐type hypersensitivity. This was confirmed by the successful achievement of CS transfer with lymphocytes from sensitized rats. When picryl chloride was used as a sensitizer instead of TDI, no comparable ear swelling was induced irrespective of the sensitization method. It was also found that topical application of a corticosteroid cream dramatically reduced the ear swelling induced by TDI. These results suggest not only that an experimental model for CS can be developed in rats using TDI, but also that CS induced by TDI can be used as a model for evaluation of anti‐CS drugs.
Genetic Control of Sulbenicillin (SBPC)‐Induced T Cell Proliferation in Mice
Authors: Ikezawa Zenro , Kawaguchi Hiroshi , Sato Masahiro 
Published: April 2015

AbstractThe major histocompatibility complex (MHC) control of T cell‐mediated response to drug‐derived determinants is not well understood. As a first step in studying the MHC control of drug hypersensitivity, semisynthetic penicillin, sulbenicillin (SBPC), was used as an antigen to establish an in vitro proliferative assay for mouse T cell response to this drug. The lymph node cells taken 7–9 days after immunization with 10 mg SBPC in complete adjuvant were tested for SBPC‐specific response in a standard 3 day proliferation assay. SBPC‐induced proliferation of the primed lymph node cells was mediated by the Thy‐1+, Lyt‐1+2– cells, i.e., helper type T (Th) cells. The Lyt‐1+2–, T cell proliferative responsiveness to SBPC was mapped on the I‐A locus within the H‐2 MHC regions. The proliferation was inhibited by the monoclonal antibody against Aβ molecules, product of the I‐A locus, but not by the monoclonal antibody against Eα molecules, product of the I‐E locus. These results indicate that the SBPC response of Lyt‐1+2–, Th cells is controlled by the I‐A locus, the products of which, the MHC class II A (AβAα) molecules, function as restriction molecules in the Th cell recognition of SBPC.The removal of the Lyt‐2+ suppressor type T (Ts) subset from the SBPC nonresponder cultures by a positive Lyt‐separation did not reverse the I‐A allele‐related nonresponsiveness. This result suggests that the nonresponsiveness is not due to the presence of the Lyt‐2+ Ts subset, but rather to the absence of the Lyt‐1+ Th subset, which recognizes SBPC together with self A molecules on the antigen presenting cell and proliferates in the SBPC nonresponder cultures.
Malignant Hemangioendothelioma in Discoid Lupus Erythematosus
Authors: Sarashi Chizuko , Nishioka Kiyoshi , Maeda Motomu , Fujikawa Masakazu , Matsumoto Koreaki , Sano Shigeharu 
Published: April 2015

AbstractA 51‐year‐old gardener developed malignant hemangioendothelioma in a lesion of discoid lupus erythematosus (DLE) after repeated occurrence of squamous cell carcinoma and keratoacanthoma. The hemangioendothelioma arose in the same DLE lesion where squamous cell carcinoma had appeared. It was treated with surgical exision followed by intraveneous dimethyltriazenoimidazole carboxamide (DTIC). He has not shown any signs of recurrence for a follow‐up period of 45 months. This is the first report, to our knowledge, of the occurrence of malignant hemangioendothelioma in a DLE lesion.
Basal Cell Epithelioma with Dermal Melanocytes
Authors: Ono Tomomichi , Fallas Victor Hugo , Higo Junko 
Published: April 2015

AbstractWe observed dermal melanocytes running in parallel with collagen fibers in the peritumoral stroma of a basal cell epithelioma. The dermal melanocyte contained many free mature melanosomes. They frequently showed an intimate contact with elastic fibers and were surrounded by an extracellular sheath with elastic fibers. These cells may have occurred due to the activation of dormant dermal melanocytes following the appearance of basal cell epithelioma, which may have been derived from a nevoid tumor originating from dormant, latent embryonic foci.