bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP
Authors: Mears Megan C. , Olivier Timothy L. , Williams-Coplin Dawn , Espinoza Edna , Bakre Abhijeet 
Published: August 2024

AbstractNewcastle disease (ND), an economically important disease in poultry, is caused by virulent strains of the genetically diverse Orthoavulavirus javaense (OAVJ). Laboratories rely on quantitative real-time reverse transcription PCR (qRT-PCR) to detect OAVJ and differentiate between OAVJ pathotypes. This study demonstrates that a fusion cleavage site based molecular beacon with reverse transcription loop mediated isothermal amplification (MB-RT-LAMP) assay can detect and differentiate OAVJ pathotypes in a single assay. Data show that the assay can rapidly identify diverse OAVJ genotypes with sensitivity only one log-fold lower than the current fusion qRT-PCR assay (104 copies), exhibits a high degree of specificity for OAVJ, and the molecular beacon can differentiate mesogenic/velogenic sequences from lentogenic sequences. Further, data show that a two-minute rapid lysis protocol preceding MB-RT-LAMP can detect and differentiate OAVJ RNA from both spiked samples and oropharyngeal swabs without the need for RNA isolation. As the MB-RT-LAMP assay can rapidly detect and discriminate between lentogenic and mesogenic/velogenic sequences of OAVJ within one assay, without the need for RNA isolation, and is adaptable to existing veterinary diagnostic laboratory workflow without additional equipment, this assay could be a rapid primary screening tool before qRT-PCR based validation in resource limited settings.
Serum levels of biomarkers related to severity staging of Raynaud’s phenomenon, neurosensory manifestations, and vibration exposure in patients with hand-arm vibration injury
Authors: Tekavec Eva , Nilsson Tohr , Dahlin Lars B. , Huynh Elizabeth , Nordander Catarina , Riddar Jakob , Kåredal Monica 
Published: August 2024

AbstractOur aim was to explore possible relationships between serum levels of biomarkers in patients with hand-arm vibration injury in relation to the severity of the vascular, i.e., Raynaud’s phenomenon (RP), and neurosensory manifestations, the current exposure level, and the duration of exposure. This study was of case series design and involved 92 patients diagnosed with hand-arm vibration injury. Jonckheere’s trend test was used to assess any association between serum levels of biomarkers and RP as well as neurosensory manifestations, graded by the International Consensus Criteria. Generalized linear models with adjustment for possible confounders were also used for associations between serum levels of biomarkers and; (1) severity of RP recorded as the extent of finger blanching calculated with Griffin score, (2) vibration perception thresholds, (3) magnitude of current exposure as [A(8); (m/s2)] value, and (4) the duration of exposure in years. Serum levels of thrombomodulin, von Willebrand factor, calcitonin gene related peptide (CGRP), heat shock protein 27, and caspase-3 were positively associated with severity of RP. Serum levels of CGRP were positively associated with the neurosensory component. No associations with exposure were shown for these biomarkers. For Intercellular adhesion molecule 1 and monocyte chemoattractant protein 1, no associations were found with neither severity nor exposure. Levels of serum biomarkers associated with endothelial injury or dysfunction, inflammation, vasodilation, neuroprotection, and apoptosis were positively associated with the severity of hand-arm vibration injury.
Analysis of homodimer formation in 12-oxophytodienoate reductase 3 in solutio and crystallo challenges the physiological role of the dimer
Authors: Kerschbaumer Bianca , Macheroux Peter , Bijelic Aleksandar 
Published: August 2024

Abstract12-oxophytodienoate reductase 3 (OPR3) is a key enzyme in the biosynthesis of jasmonoyl-L-isoleucine, the receptor-active form of jasmonic acid and crucial signaling molecule in plant defense. OPR3 was initially crystallized as a self-inhibitory dimer, implying that homodimerization regulates enzymatic activity in response to biotic and abiotic stresses. Since a sulfate ion is bound to Y364, mimicking a phosphorylated tyrosine, it was suggested that dimer formation might be controlled by reversible phosphorylation of Y364 in vivo. To investigate OPR3 homodimerization and its potential physiological role in more detail, we performed analytical gel filtration and dynamic light scattering on wild-type OPR3 and three variants (R283D, R283E, and Y364P). The experiments revealed a rapid and highly sensitive monomer–dimer equilibrium for all OPR3 constructs. We crystallized all constructs with and without sulfate to examine its effect on the dimerization process and whether reversible phosphorylation of Y364 triggers homodimerization in vivo. All OPR3 constructs crystallized in their monomeric and dimeric forms independent of the presence of sulfate. Even variant Y364P, lacking the putative phosphorylation site, was crystallized as a self-inhibitory homodimer, indicating that Y364 is not required for dimerization. Generally, the homodimer is relatively weak, and our results raise doubts about its physiological role in regulating jasmonate biosynthesis.
Controlled labelling of tracer antibodies for time-resolved fluorescence-based immunoassays
Authors: Kushnarova-Vakal Anastasiia , Aalto Rami , Huovinen Tuomas , Wittfooth Saara , Lamminmäki Urpo 
Published: August 2024

AbstractTracer antibodies, which are labelled with fluorescent or other type of reporter molecules, are widely employed in diagnostic immunoassays. Time-resolved fluorescence immunoassay (TRFIA), recognized as one of the most sensitive immunoassay techniques, utilizes tracers labelled with lanthanide ion (Ln) chelates. The conventional approach for conjugating isothiocyanate (ITC) Ln-chelates to antibodies involves random chemical targeting of the primary amino group of Lys residues, requiring typically overnight exposure to an elevated pH of 9–9.3 and leading to heterogeneity. Moreover, efforts to enhance the sensitivity of the assays by introducing a higher number of Ln-chelates per tracer antibody are associated with an elevated risk of targeting critical amino acid residues in the binding site, compromising the binding properties of the antibody. Herein, we report a method to precisely label recombinant antibodies with a defined number of Ln-chelates in a well-controlled manner by employing the SpyTag/SpyCatcher protein ligation technology. We demonstrate the functionality of the method with a full-length recombinant antibody (IgG) as well as an antibody fragment by producing site-specifically labelled antibodies for TRFIA for cardiac troponin I (cTnI) detection with a significant improvement in assay sensitivity compared to that with conventionally labelled tracer antibodies. Overall, our data clearly illustrates the benefits of the site-specific labelling strategy for generating high-performing tracer antibodies for TRF immunoassays.
Impact of climate-smart agriculture practices on multidimensional poverty among coastal farmers in Bangladesh
Authors: Islam Md. Karimul , Farjana Fariha 
Published: August 2024

AbstractClimate-smart agriculture technology helps safeguard crop income from climate change-induced crop loss. Here we identify the factors determining the adoption of climate-smart agriculture and the impact of its adoption on multidimensional poverty among coastal climate-vulnerable farm households. We employ full information maximum likelihood estimation under the endogenous switching regression approach to account for counterfactual scenarios. Results indicate that the decision to adopt climate-smart agriculture is influenced by crop vulnerability, crop income, access to extension service, and training on input management. The current adopters of climate-smart agriculture experience a 41-percentage point reduction in multidimensional poverty compared to if they had not adopted this technique. Likewise, if the current non-adopters adopted climate-smart technology they could reduce poverty by 15 percentage points. Findings also claim that some specific climate-smart technologies are particularly effective in reducing poverty, providing valuable information to coastal farmers in making informed decisions about which technologies may be effective.
Robots are both anthropomorphized and dehumanized when harmed intentionally
Authors: Wieringa Marieke S. , Müller Barbara C. N. , Bijlstra Gijsbert , Bosse Tibor 
Published: August 2024

AbstractThe harm-made mind phenomenon implies that witnessing intentional harm towards agents with ambiguous minds, such as robots, leads to augmented mind perception in these agents. We conducted two replications of previous work on this effect and extended it by testing if robots that detect and simulate emotions elicit a stronger harm-made mind effect than robots that do not. Additionally, we explored if someone is perceived as less prosocial when harming a robot compared to treating it kindly. The harm made mind-effect was replicated: participants attributed a higher capacity to experience pain to the robot when it was harmed, compared to when it was not harmed. We did not find evidence that this effect was influenced by the robot’s ability to detect and simulate emotions. There were significant but conflicting direct and indirect effects of harm on the perception of mind in the robot: while harm had a positive indirect effect on mind perception in the robot through the perceived capacity for pain, the direct effect of harm on mind perception was negative. This suggests that robots are both anthropomorphized and dehumanized when harmed intentionally. Additionally, the results showed that someone is perceived as less prosocial when harming a robot compared to treating it kindly.
Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics
Authors: Wright Madison T , Timalsina Bibek , Garcia Lopez Valeria , Hermanson Jake N , Garcia Sarah , Plate Lars 
Published: August 2024

AbstractMany cellular processes are governed by protein–protein interactions that require tight spatial and temporal regulation. Accordingly, it is necessary to understand the dynamics of these interactions to fully comprehend and elucidate cellular processes and pathological disease states. To map de novo protein–protein interactions with time resolution at an organelle-wide scale, we developed a quantitative mass spectrometry method, time-resolved interactome profiling (TRIP). We apply TRIP to elucidate aberrant protein interaction dynamics that lead to the protein misfolding disease congenital hypothyroidism. We deconvolute altered temporal interactions of the thyroid hormone precursor thyroglobulin with pathways implicated in hypothyroidism pathophysiology, such as Hsp70-/90-assisted folding, disulfide/redox processing, and N-glycosylation. Functional siRNA screening identified VCP and TEX264 as key protein degradation components whose inhibition selectively rescues mutant prohormone secretion. Ultimately, our results provide novel insight into the temporal coordination of protein homeostasis, and our TRIP method should find broad applications in investigating protein-folding diseases and cellular processes.
The MondoA-dependent TXNIP/GDF15 axis predicts oxaliplatin response in colorectal adenocarcinomas
Authors: Deng Jinhai , Pan Teng , Wang Dan , Hong Yourae , Liu Zaoqu , Zhou Xingang , An Zhengwen , Li Lifeng , Alfano Giovanna , Li Gang , Dolcetti Luigi , Evans Rachel , Vicencio Jose M , Vlckova Petra , Chen Yue , Monypenny James , Gomes Camila Araujo De Carvalho , Weitsman Gregory , Ng Kenrick , McCarthy Caitlin , Yang Xiaoping , Hu Zedong , Porter Joanna C , Tape Christopher J , Yin Mingzhu , Wei Fengxiang , Rodriguez-Justo Manuel , Zhang Jin , Tejpar Sabine , Beatson Richard , Ng Tony 
Published: August 2024

AbstractChemotherapy, the standard of care treatment for cancer patients with advanced disease, has been increasingly recognized to activate host immune responses to produce durable outcomes. Here, in colorectal adenocarcinoma (CRC) we identify oxaliplatin-induced Thioredoxin-Interacting Protein (TXNIP), a MondoA-dependent tumor suppressor gene, as a negative regulator of Growth/Differentiation Factor 15 (GDF15). GDF15 is a negative prognostic factor in CRC and promotes the differentiation of regulatory T cells (Tregs), which inhibit CD8 T-cell activation. Intriguingly, multiple models including patient-derived tumor organoids demonstrate that the loss of TXNIP and GDF15 responsiveness to oxaliplatin is associated with advanced disease or chemotherapeutic resistance, with transcriptomic or proteomic GDF15/TXNIP ratios showing potential as a prognostic biomarker. These findings illustrate a potentially common pathway where chemotherapy-induced epithelial oxidative stress drives local immune remodeling for patient benefit, with disruption of this pathway seen in refractory or advanced cases.
Inhibition of GSK3α,β rescues cognitive phenotypes in a preclinical mouse model of CTNNB1 syndrome
Authors: Alexander Jonathan M , Vazquez-Ramirez Leeanne , Lin Crystal , Antonoudiou Pantelis , Maguire Jamie , Wagner Florence , Jacob Michele H 
Published: August 2024

AbstractCTNNB1 syndrome is a rare monogenetic disorder caused by CTNNB1 de novo pathogenic heterozygous loss-of-function variants that result in cognitive and motor disabilities. Treatment is currently lacking; our study addresses this critical need. CTNNB1 encodes β-catenin which is essential for normal brain function via its dual roles in cadherin-based synaptic adhesion complexes and canonical Wnt signal transduction. We have generated a Ctnnb1 germline heterozygous mouse line that displays cognitive and motor deficits, resembling key features of CTNNB1 syndrome in humans. Compared with wild-type littermates, Ctnnb1 heterozygous mice also exhibit decreases in brain β-catenin, β-catenin association with N-cadherin, Wnt target gene expression, and Na/K ATPases, key regulators of changes in ion gradients during high activity. Consistently, hippocampal neuron functional properties and excitability are altered. Most important, we identify a highly selective inhibitor of glycogen synthase kinase (GSK)3α,β that significantly normalizes the phenotypes to closely meet wild-type littermate levels. Our data provide new insights into brain molecular and functional changes, and the first evidence for an efficacious treatment with therapeutic potential for individuals with CTNNB1 syndrome.
Short‐term electric power and energy balance optimization scheduling based on low‐carbon bilateral demand response mechanism from multiple perspectives
Authors: Li Juan , Li Yonggang , Liu Huazhi 
Published: August 2024

AbstractCarbon emissions limit the output of traditional fuel‐fired generating units, significantly affecting the new power system scheduling mechanism. This paper proposes a short‐term electric power and energy balance optimization scheduling method with low‐carbon bilateral demand response (LCBDR). The LCBDR mechanism framework is constructed by combining the analysis of short‐term electric power and energy balance of the system under a dual perspective, along with the electric‐carbon coupling mechanism of the dynamic scheduling on the source‐load side. Based on the carbon emission flow (CEF) theory, the carbon emission index information of load‐side users is obtained. An optimal scheduling model of LCBDR is established. The enhanced decision tree classifier (EDTC) algorithm is used to predict the electricity consumption behavior of transferable load (TL) users, and an improved particle swarm optimization (PSO) algorithm with “ε‐greedy” strategy is proposed to solve this model. Comprehensive case studies from three different perspectives verify that this method can effectively realize the low‐carbon economic operation of the system, with the peak net load reduced by 24.02% and valley net load increased by 20.43%. Compared with a single perspective, the total operational costs can be reduced by 5.27%, and the carbon emissions of users can be reduced by 5.70%.
Rocky planet formation in compact disks around M dwarfs
Authors: Sanchez M. , van der Marel N. , Lambrechts M. , Mulders G. D. , Guerra-Alvarado O. M. 
Published: August 2024

Context. Due to the improvements in radial velocity and transit techniques, we know that rocky or rocky-icy planets, in particular close-in super-Earths in compact configurations, are the most common ones around M dwarfs. On the other hand, thanks to the high angular resolution of ALMA we know that many disks around very low-mass stars (between 0.1 and 0.5 M⊙) are rather compact and small (without observable substructures and radius less than 20 au), which favors the idea of an efficient radial drift that could enhance planet formation in the terrestrial zone.
Aims. Our aim was to investigate the potential formation paths of the observed close-in rocky exoplanet population around M dwarfs, especially close-in super-Earths, assuming that planet formation could take place in compact disks with an efficient dust radial drift.
Methods. We developed N-body simulations that include a sample of embryos growing by pebble accretion exposed to planet-disk interactions, star-planet tidal interactions, and general relativistic corrections that include the evolution of the luminosity, radius, and rotational period of the star. For a star of 0.1 M⊙, we considered different gas disk viscosities and initial embryo distributions. We also explored planet formation by pebble accretion around stars of 0.3 M⊙ and 0.5 M⊙. Lastly, for each stellar mass, we ran simulations that include a sample of embryos growing by planetesimal accretion instead of pebble accretion.
Results. Our main result is that the sample of simulated planets that grow by pebble accretion in a gas disk with low viscosity (α = 10−4) can reproduce the close-in low-mass exoplanet population around M dwarfs in terms of multiplicity, mass, and semi-major axis. Furthermore, we found that a gas disk with high viscosity (α = 10−3), and thus lower pebble accretion rates, cannot reproduce the observed planet masses as no planet more massive than 0.5 M⊕ could be formed in our simulations. In addition, we show that planetesimal accretion favors the formation of smaller planets than pebble accretion does. Whether this planet population truly exists remains unknown with the current instrumental sensitivity.
Conclusions. Rocky planet formation around M dwarfs can take place in compact and small dust disks driven by an efficient radial drift in a gas disk with low viscosity (α = 10−4). This result points toward a new approach in the direction of the disk conditions needed for rocky planet formation around very low-mass stars.
Facetten von Intimität in konsensuell nicht-monogamen Liebesbeziehungen. Eine qualitative Interviewstudie
Authors: Bröning Sonja , Clüver Annette , Gebhard Klara Patrizia 
Published: August 2024

Zusammenfassung
Einleitung Bedürfnisse nach Autonomie und Verbundenheit stehen im Kontext romantischer Beziehungen in einem spannungsreichen Verhältnis. In polyamoren Beziehungsmodellen herrscht im Vergleich zu monogamen Partnerschaften eine hohe sexuelle und romantische Autonomie. Gleichzeitig muss dort Intimität trotz Nicht-Exklusivität hergestellt werden.
Forschungsziele Wir explorieren die Alltagspraxis der Intimität in konsensuell nicht-monogamen Liebesbeziehungen. Wie erleben polyamor lebende Menschen ihre Simultanbeziehungen in Hinblick auf (1) Facetten von Intimität wie Selbstoffenbarung und emotionale Verbundenheit sowie (2) emotionale Distanz? Zeigen sich jeweils Unterschiede zwischen den verschiedenen Partnerschaften?
Methoden Mittels halbstandardisierter Leitfadeninterviews wurde eine Gelegenheitsstichprobe von 20 polyamor lebenden Individuen (M
Alter = 33.15, 70 % weiblich, 35 % bisexuell, 30 % pansexuell, 70 % kinderlos) befragt. Verglichen wurden Facetten von Intimität und Distanz (1) in der Beziehung zu der engsten Bindungsperson (operationalisiert als Partner*in, der*die im Falle einer persönlichen Krise als Erstes kontaktiert werden würde) und (2) in der zweitwichtigsten Liebesbeziehung. Die Auswertung erfolgte mittels qualitativer Inhaltsanalyse mit induktiver Kategorienbildung. Zusätzlich wurde Bindungssicherheit im Selbstbericht erfasst.
Ergebnisse Die Teilnehmenden wiesen insgesamt eine hohe Bindungssicherheit auf und beschrieben ein hohes Maß an emotionaler Verbundenheit mit ihren Primärpartner*innen, während sich bezüglich dieser Merkmale bei Sekundärpartner*innen ein differenzierteres Bild ergab. Bezogen auf emotionale Distanz zeigten sich in der Primärpartnerschaft mehr Absprachen und Regeln, aber auch mehr offene Kommunikation über Konflikte und Eifersucht. Sorgen bezüglich einer übermäßigen Distanz traten sowohl in Primär- als auch Sekundärpartnerschaften vielfach auf.
Schlussfolgerung Polyamorie bietet Potenzial für ein hohes Maß an Intimität in simultanen Liebesbeziehungen, setzt jedoch ausgeprägte Kompetenzen in der Selbstregulation und Kommunikation voraus.
Cannabisprävention in der Schule: Ergebnisse einer
cluster-randomisierten Studie
Authors: Suchert Vivien , Isensee Barbara , Goecke Michaela , Hanewinkel Reiner 
Published: August 2024

ZusammenfassungZur Ergebnisevaluation einer schulbasierten Cannabispräventionseinheit wurde eine
cluster-randomisierte Studie in fünf deutschen Bundesländern durchgeführt.
Insgesamt 55 Schulen wurden nach dem Zufallsprinzip in die Interventionsgruppe,
d. h. mit Teilnahme an einem zweistündigen Workshop zur Cannabisprävention, der
von Suchtpräventionsfachkräften in den Klassen 8 und 9 durchgeführt wurde, oder
in eine Wartelisten-Kontrollgruppe eingeteilt. Wissen, Einstellungen,
Intentionen und das Verhalten der Jugendlichen in Bezug auf Cannabis wurden bei
Studienbeginn und sechs Monate später gemessen. 2.669 Schüler (50,8% Mädchen,
Durchschnittsalter=14,12 Jahre) bilden die Stichprobe. Es konnten Auswirkungen
der Intervention auf das Wissen der Schüler (adjustierter
Regressionskoeffizient=0,26 [0,15–0,38], p<0,001) sowie auf die erwarteten
negativen Folgen des Cannabiskonsums bei Jugendlichen (adjustierter
Regressionskoeffizient=0,15 [0,04–0,25], p=0,006) festgestellt werden. Beide
Faktoren nahmen in der Interventionsgruppe im Vergleich zur Kontrollgruppe
signifikant stärker zu. Es gab keine Auswirkungen auf andere
einstellungsbezogene Variablen, Intentionen oder Verhalten. Ein sehr kurzer
schulischer Workshop kann das Wissen und die kritische Einstellung zum
Cannabiskonsum bei Schülern der Klassen 8 und 9 verbessern, hatte jedoch keinen
Einfluss auf die zukünftigen Absichten und den Cannabiskonsum.
Design of Molecular Diversity by Olefin Metathesis in Tandem with Other Reactions
Authors: Gupta Naveen Kumar , Ansari Saima , Kotha Sambasivarao , Singh Deepshikha 
Published: August 2024

AbstractThis review summarizes various strategies that combine metathesis with diverse named and unnamed reactions to create molecular diversity in producing carbocycles, macrocycles, and heterocycles.1	Introduction2	Olefinations3	Rearrangement4	Allylation5	Cycloadditions6	Coupling Reactions7	Grignard Reaction8	Radical reactions9	Conjugate Addition–Metathesis10	Multicomponent Reactions11	Miscellaneous12	Conclusions