bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic utility of aqueocentesis and aqueous humor analysis in dogs and cats with anterior uveitis
Authors: Wiggans K. Tomo , Vernau William , Lappin Michael R. , Thomasy Sara M. , Maggs David J. 
Published: August 2013

AbstractObjectiveTo evaluate diagnostic utility of aqueous humor analysis in animals with anterior uveitis.AnimalsClient‐owned dogs (n = 12) and cats (n = 10).ProceduresExamination findings and diagnostic test results including aqueous humor cytology were compared.ResultsDisease duration prior to aqueocentesis was not significantly different between dogs with idiopathic anterior uveitis and those with an etiologic diagnosis, but was shorter in cats with feline infectious peritonitis (FIP) than those with idiopathic uveitis. Microbial nucleic acids, antigens, or antibodies against them were seldom found in blood/serum; however, serum feline coronavirus titers ≥1:6400 were detected only in cats withFIP. Aqueous humor cytology was diagnostic in no cats and two dogs, both with neoplasia. Although aqueous humor contained predominantly neutrophils in cats withFIPand large reactive lymphocytes and plasma cells appeared more frequent in cats with idiopathic uveitis, neither clinical nor cytologic assessment of anterior chamber contents differed significantly between cats with idiopathic orFIP‐associated uveitis. Cytologically assessed plasma cell number was correlated with keratic precipitates and disease duration. Clinically detectable hyphema and cytologic erythrocyte number were correlated. However, cytologic cell grades and clinical grade of flare or cell numbers within the anterior chamber were not correlated.ConclusionsAqueous humor cytology permitted diagnosis of neoplasia in dogs with anterior uveitis but was generally not helpful in cats. Poor correlation between clinical and cytologic assessment of cell numbers and type within the anterior chamber dictates that clinical grading should not be the sole criterion for electing to perform aqueocentesis.
Evaluation of the conjunctival fungal flora and its susceptibility to antifungal agents in healthy horses in Switzerland
Authors: Voelter‐Ratson Katrin , Monod Michel , Unger Lucia , Spiess Bernhard M. , Pot Simon A. 
Published: August 2013

AbstractObjectiveTo characterize the conjunctival fungal flora and to determine the susceptibility of 2 isolated molds to antifungal drugs in samples of 64 healthy horses from The National Stud in Switzerland.ProcedureConjunctival cytobrush samples were collected from both eyes of 64 ophthalmologically normal horses in August 2012 and subsequently cultured on Sabouraud's agar medium. Growing fungi were identified and counted. Etests or broth microdilution tests forAspergillus fumigatusandEurotium amstelodamiwere carried out to determine antifungal drug sensitivity. These species had previously been detected in samples from eyes with keratomycosis in Switzerland. Minimal inhibitory concentrations (MICs) for voriconazole, fluconazole, itraconazole, amphotericin B, and miconazole were recorded.ResultsFifty‐nine of the horses were tested positive for fungal growth from at least one eye (92%). Eleven genera of fungi were identified. The most common fungal genera wereAlternaria,Eurotium, Rhizopus,andCladosporium.Aspergillusspp. andPenicilliumspp. were isolated frequently, while noFusariumspp. was found. In only 2 cases, yeasts were identified asCandida guilliermondii. For certain fungal species, the type of bedding and housing appeared to influence their prevalence. Susceptibility testing ofA. fumigatusshowed lowestMICs for voriconazole,E. amstelodamifor voriconazole and itraconazole. HighMICs for fluconazole were detected for all tested fungi whileMICs for amphotericin B and miconazole were variable.ConclusionsA large range of fungal mold species was identified includingA. fumigatusandE. amstelodami, which have been causative agents of keratomycosis in Switzerland. Bestin vitrosusceptibility results for these two species were obtained for voriconazole.
Draft Genome Sequence of an Oscillatorian Cyanobacterium, Strain ESFC-1
Authors: Everroad R. Craig , Woebken Dagmar , Singer Steven W. , Burow Luke C. , Kyrpides Nikos , Woyke Tanja , Goodwin Lynne , Detweiler Angela , Prufert-Bebout Leslie , Pett-Ridge Jennifer 
Published: August 2013

ABSTRACT
The nonheterocystous filamentous cyanobacterium strain ESFC-1 has recently been isolated from a marine microbial mat system, where it was identified as belonging to a recently discovered lineage of active nitrogen-fixing microorganisms. Here, we report the draft genome sequence of this isolate. The assembly consists of 3 scaffolds and contains 5,632,035 bp with a GC content of 46.5%.
Complete Genome Sequence of a Papillomavirus Isolated from the European Mole
Authors: Maes Piet , Sijmons Steven , Stevens Hans , Van Ranst Marc 
Published: August 2013

ABSTRACT

A papillomavirus was isolated from healthy epithelial tissue of two European moles (
Talpa europaea
) and the complete genomic sequence was determined. To our knowledge, this is the first papillomavirus to be isolated from a mole. Phylogenetic analysis shows it to be most closely related to viruses of the genus
Kappapapillomavirus
.
Complete Genome Sequence of Lactobacillus plantarum Strain 16, a Broad-Spectrum Antifungal-Producing Lactic Acid Bacterium
Authors: Crowley Sarah , Bottacini Francesca , Mahony Jennifer , van Sinderen Douwe 
Published: August 2013

ABSTRACT

Lactobacillus plantarum
strain 16 restricts the growth of various food spoilage fungi and has the potential to be used as a biopreservative to improve the shelf life of foods. The complete genome sequence contains 3,044,678 bp with a G+C content of 44.74% and harbors the largest plasmid complement reported for this species to date.
De Novo
Assembly of the
Streptomyces
sp. Strain Mg1 Genome Using PacBio Single-Molecule Sequencing
Authors: Hoefler B. Christopher , Konganti Kranti , Straight Paul D. 
Published: August 2013

ABSTRACT

We report a draft genome assembly of
Streptomyces
sp. strain Mg1, a competitive soil isolate with multiple secondary metabolite gene clusters.
Draft Genome Sequence of a Clinical Isolate of Multidrug-Resistant Mycobacterium tuberculosis East African Indian Strain OSDD271
Authors: Karuthedath Vellarikkal Shamsudheen , Patowary Ashok , Singh Meghna , Periwal Vinita , Singh Ajay Vir , Singh Pravin Kumar , Garg Parul , Mohan Katoch Viswa , Katoch Kiran , Jangir Pramod Kumar , Sharma Rakesh ,   Open Source Drug Discovery Consortium, Chauhan D. S. , Scaria Vinod , Sivasubbu Sridhar 
Published: August 2013

ABSTRACT

We describe the genome sequencing and analysis of a clinical isolate of
Mycobacterium tuberculosis
East African Indian (EAI) strain OSDD271 from India.
Genome Sequence of Clostridium diolis Strain DSM 15410, a Promising Natural Producer of 1,3-Propanediol
Authors: Wang Yu , Tao Fei , Tang Hongzhi , Xu Ping 
Published: August 2013

ABSTRACT

Clostridium diolis
strain DSM 15410 is considered one of the best natural producers of 1,3-propanediol because of its appreciable substrate-tolerant ability, yield, and productivity. Here, we present a 5.85-Mb assembly of its genome sequence. We have annotated the coding sequences responsible for glycerol utilization and 1,3-propanediol fermentation.
Complete Genome Sequence of a Reassortant H14N2 Avian Influenza Virus from California
Authors: Boyce Walter M. , Schobel Seth , Dugan Vivien G. , Halpin Rebecca , Lin Xudong , Wentworth David E. , Lindsay LeAnn L. , Mertens Eva , Plancarte Magdalena 
Published: August 2013

ABSTRACT
We report the complete genome sequence of a reassortant H14N2 avian influenza virus isolated in 2011 from a northern shoveler in California. This introduced Eurasian subtype acquired seven segments from North American viruses and circulated in the Pacific Flyway 1 year after its detection in the Mississippi Flyway.
Complete Genome Sequence of
Enterobacter
sp. Strain R4-368, an Endophytic N-Fixing Gammaproteobacterium Isolated from Surface-Sterilized Roots of
Jatropha curcas
L
Authors: Madhaiyan Munusamy , Peng Ni , Ji Lianghui 
Published: August 2013

ABSTRACT

Enterobacter
sp. strain R4-368 is one of the few characterized
Jatropha
endophytic diazotrophic bacteria and was isolated from surface-sterilized roots. This bacterium shows strong growth-promoting effects, being able to increase plant biomass and seed yields.
Enterobacter
sp. R4-368 is the second fully sequenced diazotrophic
Enterobacter
species. The sequence information shall facilitate the elucidation of the molecular mechanisms of plant growth promotion, nitrogen fixation in nonlegume plant species, and evolution of biological nitrogen fixation systems.
Draft Genome Sequence of the Bisphenol A-Degrading Bacterium
Sphingobium
sp. Strain YL23
Authors: Hu Anyi , Lv Min , Yu Chang-Ping 
Published: August 2013

ABSTRACT

Sphingobium
sp. strain YL23, a novel bacterium isolated from sewage sludge of a domestic wastewater treatment plant, has been shown to completely degrade bisphenol A under aerobic conditions. Here, we describe a 3.8-Mb assembly of its genome sequence and major findings from its annotation.
Draft Genome Sequence of Pseudomonas putida Strain MTCC5279
Authors: Chaudhry Vasvi , Asif Mehar H. , Bag Sumit , Goel Ridhi , Mantri Shrikant S. , Singh Sunil K. , Chauhan Puneet S. , Sawant Samir V. , Nautiyal Chandra Shekhar 
Published: August 2013

ABSTRACT

Here we report the genome sequence of a plant-growth-promoting rhizobacterium,
Pseudomonas putida
strain MTCC5279. The length of the draft genome sequence is approximately 5.2 Mb, with a GC content of 62.5%. The draft genome sequence reveals a number of genes whose products are possibly involved in plant growth promotion and abiotic stress tolerance.
Draft Genome Sequence of Bacillus cereus Strain F, Isolated from Ancient Permafrost
Authors: Brenner Evgeniy V. , Brouchkov Anatoli V. , Kurilshikov Alexander M. , Griva Gennady I. , Kashuba Elena , Kashuba Vladimir I. , Melefors O. , Repin Vladimir E. , Melnikov Vladimir P. , Vlassov Valentin V. 
Published: August 2013

ABSTRACT

Bacillus cereus
strain F was isolated and cultured from a sample of permafrost, aged presumably about 3 million years, on the Mammoth Mountain (62°56′N, 133°59′E). These genome data provide the basis to investigate
Bacillus cereus
F, identified as a long-term survivor of the extremely cold and close environment.
Complete Genome Sequence of Persistent Cystic Fibrosis Isolate Pseudomonas aeruginosa Strain RP73
Authors: Jeukens Julie , Boyle Brian , Bianconi Irene , Kukavica-Ibrulj Irena , Tümmler Burkhard , Bragonzi Alessandra , Levesque Roger C. 
Published: August 2013

ABSTRACTPseudomonas aeruginosacan establish lifelong chronic airway infections in cystic fibrosis (CF) patients. However, the genetic features associated with long-term persistence in the lung are not understood. We sequenced the genome ofP. aeruginosastrain RP73, which was isolated after 16.9 years of chronic lung infection in a CF patient.
Genome Sequence of Paenibacillus polymyxa ATCC 12321, a Promising Strain for Optically Active (
R
,
R
)-2,3-Butanediol Production
Authors: Tong Ying-Jia , Ji Xiao-Jun , Liu Lu-Gang , Shen Meng-Qiu , Huang He 
Published: August 2013

ABSTRACT

Paenibacillus polymyxa
is a potential strain for (
R
,
R
)-2,3-butanediol production. Here, we report an annotated draft genome sequence of
P. polymyxa
strain ATCC 12321, which contains 4,429 protein-coding genes and 49 structural RNAs. This genome sequence provides a genetic basis for a better understanding of the mechanism for the accumulation of highly optically active (
R
,
R
)-2,3-butanediol.
Genome Sequence of Porcine Escherichia coli Strain IMT8073, an Atypical Enteropathogenic E. coli Strain Isolated from a Piglet with Diarrhea
Authors: Semmler Torsten , Eichhorn Inga , Bethe Astrid , Bauerfeind Rolf , Pickard Derek , Kingsley Robert A. , Dougan Gordon , Wieler Lothar H. 
Published: August 2013

ABSTRACT

Escherichia coli
is a highly diverse bacterial species, with atypical enteropathogenic
E. coli
(aEPEC) causing intestinal disease in both human and animal hosts. Here, we report the first complete genome sequence of an aEPEC strain of sequence type ST794 and serotype Ont:H7, isolated from a diseased piglet.
Trapping and Identification of Cellular Substrates of the Staphylococcus aureus ClpC Chaperone
Authors: Graham Justin W. , Lei Mei G. , Lee Chia Y. 
Published: August 2013

ABSTRACT

ClpC is an ATP-dependent Hsp100/Clp chaperone involved in protein quality control in low-GC Gram-positive bacteria. Previously, we found that ClpC affected the expression of a large number of genes, including capsule genes in
Staphylococcus aureus
. Here we constructed a His-tagged ClpC variant (ClpC
trap
) with mutations within the Walker B motifs to identify the direct substrates of ClpC by copurification with ClpC
trap
followed by gel electrophoresis combined with liquid chromatography-tandem mass spectrometry proteomics. We identified a total of 103 proteins that are potential substrates of ClpC in strain Newman. The direct protein-protein interaction of ClpC with a subset of the captured proteins was verified in a bacterial two-hybrid system. The captured proteins could be grouped into various functional categories, but most were related to proteins involved in the stress response. Several known ClpC substrates were captured, including ClpP, TrfA/MecA, ClpB, DnaK, DnaJ, GroL, RecA, and CodY, supporting the validity of our approach. Our results also revealed many new ClpC substrates, including AgrA, CcpA, RsbW, MurG, FtsA, SrtA, Rex, Atl, ClfA, and SbcC. Analysis of capsule production showed that three of the captured proteins, which were not previously known to be transcriptional regulators, did affect capsule production.
The impact of targeting all elderly persons in England and Wales for yearly influenza vaccination: excess mortality due to pneumonia or influenza and time trend study
Authors: Mann Andrea G , Mangtani Punam , Russell Colin A , Whittaker John C 
Published: August 2013

ObjectiveTo investigate the impact on mortality due to pneumonia or influenza of the change from risk-based to age group-based targeting of the elderly for yearly influenza vaccination in England and Wales.DesignExcess mortality estimated using time series of deaths registered to pneumonia or influenza, accounting for seasonality, trend and artefacts. Non-excess mortality plotted as proxy for long-term trend in mortality.SettingEngland and Wales.ParticipantsPersons aged 65–74 and 75+ years whose deaths were registered to underlying pneumonia or influenza between 1975/1976 and 2004/2005.Outcome measuresMultiplicative effect on average excess pneumonia and influenza deaths each winter in the 4–6 winters since age group-based targeting of vaccination was introduced (in persons aged 75+ years from 1998/1999; in persons aged 65+ years from 2000/2001), estimated using multivariable regression adjusted for temperature, antigenic drift and vaccine mismatch, and stratified by dominant circulating influenza subtype. Trend in baseline weekly pneumonia and influenza death rates.ResultsThere is a suggestion of lower average excess mortality in the six winters after age group-based targeting began compared to before, but the CI for the 65–74 years age group includes no difference. Trend in baseline pneumonia and influenza mortality shows an apparent downward turning point around 2000 for the 65–74 years age group and from the mid-1990s in the 75+ years age group.ConclusionsThere is weakly supportive evidence that the marked increases in vaccine coverage accompanying the switch from risk-based to age group-based targeting of the elderly for yearly influenza vaccination in England and Wales were associated with lower levels of pneumonia and influenza mortality in older people in the first 6 years after age group-based targeting began. The possible impact of these policy changes is observed as weak evidence for lower average excess mortality as well as a turning point in baseline mortality coincident with the changes.
Screening for hypothalamic–pituitary–adrenal axis suppression in asthmatic children remains problematic: a cross-sectional study
Authors: Zöllner Ekkehard Werner , Lombard Carl J , Galal Ushma , Hough Stephen , Irusen Elvis M , Weinberg Eugene 
Published: August 2013

ObjectiveTo determine which parameter is the most useful screening test for hypothalamic–pituitary–adrenal suppression in asthmatic children.DesignCross-sectional study.SettingPaediatric allergy clinics in Cape Town, South Africa.Participants143 asthmatic children of mostly mixed ancestry, aged 5–12 years.Outcome measuresPrimary outcome measures included Spearman correlation coefficients (r) calculated between the postmetyrapone (PMTP) serum adrenocorticotropic hormone (ACTH), 11-deoxycortisol (11DOC), 11DOC+ cortisol (C) and height, weight, height velocity, weight velocity, change in systolic blood pressure from supine to standing, early morning urinary free cortisol (UFC), morning C, ACTH and dehydroepiandrosterone sulfate (DHEAS). Secondary outcome measures were the receiver operating characteristics (ROC) curve and the diagnostic statistics for the most promising test.ResultsAll screening variables were weakly correlated with the three PMTP outcomes. Only DHEAS and UFC (nmol/m2) were statistically significant—DHEAS for PMTP ACTH and 11DOC (r=0.20, p=0.025 and r=0.21, p=0.017); UFC (nmol/m2) for PMTP 11DOC and 11DOC+C (r=0.19, p=0.033 and r=0.20, p=0.022). The area under ROC curve for DHEAS in the 5-year to 9-year age group was 0.69 (95% CI 0.47 to 0.92). At DHEAS cut-off of 0.2 µmol/L: sensitivity=0.88 (CI 0.47 to 1.00), specificity=0.61 (CI 0.42 to 0.78), positive predictive value=0.37 (CI 0.16 to 0.62), negative predictive value=0.95 (CI 0.75 to 1.00), accuracy=0.67 (CI 0.50 to 0.81), positive likelihood ratio=2.26 (CI 1.35 to 3.78), negative likelihood ratio=0.20 (CI 0.03 to 1.30).ConclusionsNo parameter is useful as a universal screening test. DHEAS may be suitable to exclude HPAS before adrenarche. Further research is needed to confirm these findings and identify factors, for example, genetic that may predict or protect against HPAS.
Predictors of fracture from falls reported in hospital and residential care facilities: a cross-sectional study
Authors: Chari Satyan , McRae Prue , Varghese Paul , Ferrar Kaye , Haines Terry P 
Published: August 2013

ObjectivesFall-related fractures are associated with substantial human and economic costs. An improved understanding of the predictors of fall-related fractures in healthcare settings would be useful in developing future interventions. The objective of this study was to identify such predictors by exploring associations between fall-related factors and fracture outcomes through logistic regression analysis of routinely collected fall incident data.DesignRetrospective cross-sectional study.Setting197 public healthcare facilities in Queensland, Australia.ParticipantsWe included data from incident reports completed after falls among admitted adult hospital patients (n=24 218 falls, 229 fractures) and aged-care residents (n=8980 falls, 74 fractures) between January 2007 and November 2009.Primary and secondary outcomesThe outcomes of interest were fall-related predictors of fracture.ResultsHospital patients who reported to have been screened for their risk of falling at admission were less likely to fracture after a fall (OR: 0.60, 95% CI 0.41 to 0.89) than those who had not been screened. Further, falls from standing (OR: 2.08, 95% CI 1.22 to 3.55) and falls while walking (OR: 1.86, 95% CI 1.32 to 2.62) were associated with higher fracture odds than falls during other activities. In line with these results, falls while reaching in standing (OR: 3.51, 95% CI 1.44 to 8.56) and falls while walking (OR: 2.11, 95% CI 1.24 to 3.58) were also predictive of fracture in the adjusted residential care model.ConclusionsOur findings indicate that screening of hospital patients for their risk of falling may contribute towards the prevention of fall-related injury. Falls from upright postures appear to be more likely to result in fractures than other falls in healthcare settings. Further prospective research is warranted.
Identifying future ‘unexpected’ survivors: a retrospective cohort study of fatal injury patterns in victims of improvised explosive devices
Authors: Singleton James A G , Gibb Iain E , Hunt Nicholas C A , Bull Anthony M J , Clasper Jonathan C 
Published: August 2013

ObjectivesTo identify potentially fatal injury patterns in explosive blast fatalities in order to focus research and mitigation strategies, to further improve survival rates from blast trauma.DesignRetrospective cohort study.ParticipantsUK military personnel killed by improvised explosive device (IED) blasts in Afghanistan, November 2007–August 2010.SettingUK military deployment, through NATO, in support of the International Security Assistance Force (ISAF) mission in Afghanistan.Data sourcesUK military postmortem CT records, UK Joint Theatre Trauma Registry and associated incident data.Main outcome measuresPotentially fatal injuries attributable to IEDs.ResultsWe identified 121 cases, 42 mounted (in-vehicle) and 79 dismounted (on foot), at a point of wounding. There were 354 potentially fatal injuries in total. Leading causes of death were traumatic brain injury (50%, 62/124 fatal injuries), followed by intracavity haemorrhage (20.2%, 25/124) in the mounted group, and extremity haemorrhage (42.6%, 98/230 fatal injuries), junctional haemorrhage (22.2%, 51/230 fatal injuries) and traumatic brain injury (18.7%, 43/230 fatal injuries) in the dismounted group.ConclusionsHead trauma severity in both mounted and dismounted IED fatalities indicated prevention and mitigation as the most effective strategies to decrease resultant mortality. Two-thirds of dismounted fatalities had haemorrhage implicated as a cause of death that may have been anatomically amenable to prehospital intervention. One-fifth of the mounted fatalities had haemorrhagic trauma which currently could only be addressed surgically. Maintaining the drive to improve all haemostatic techniques for blast casualties, from point of wounding to definitive surgical proximal vascular control, alongside the development and application of novel haemostatic interventions could yield a significant survival benefit. Prospective studies in this field are indicated.
Adrenocortical tumours: high CT attenuation value correlates with eosinophilia but does not discriminate lipid-poor adenomas from malignancy
Authors: Pennanen Mirkka , Raade Merja , Louhimo Johanna , Sane Timo , Heiskanen Ilkka , Arola Johanna , Haglund Caj 
Published: August 2013

BackgroundCharacterisation of adrenal tumours is an important clinical problem. Unenhanced CT is the primary imaging modality to assess the nature of these lesions.AimsTo study the correlation between unenhanced CT attenuation value and the specific histopathology, as well as the proportion of lipid-poor eosinophilic cells in adrenocortical tumours.MethodsWe studied retrospectively primary adrenocortical tumours that had been operated on at Helsinki University Central Hospital between 2002 and 2008. Of 171 tumours, 79 had appropriate preoperative CT scans and were included in the study. We evaluated the unenhanced CT attenuation values (Hounsfield units, HU) of these tumours and determined their histopathological diagnosis by the Weiss scoring system. We also assessed the proportion of lipid-poor eosinophilic cells for each tumour.ResultsUnenhanced CT attenuation value (HU) in adrenocortical tumours correlated well with the proportion of lipid-poor eosinophilic cells (rs=0.750, p<0.001). HU and Weiss score also had a correlation (rs=0.582, p<0.001).ConclusionsUnenhanced CT attenuation value correlates well with the percentage of lipid-poor eosinophilic cells, but unenhanced CT attenuation value fails to differentiate between benign lipid-poor adenomas and malignant adrenocortical tumours. All adrenocortical tumours with unenhanced CT attenuation value ≤10 HU are histologically benign lipid-rich tumours.