bluuebunny/crossref_metadata_2025_split huggingface dataset:
Estimation of potential social support requirement for tuberculosis patients in India
Authors: Chatterjee Susmita , Stallworthy Guy , Das Palash , Vassall Anna 
Published: July 2024

Abstract
Providing social support to tuberculosis (TB) patients is a recommended strategy as households having TB patients find themselves in a spiral of poverty because of high cost, huge income loss and several other economic consequences associated with TB treatment. However, there are few examples of social support globally. The Indian government introduced the ‘Nikshay Poshan Yojana’ scheme in 2018 to provide nutritional support for all registered TB patients. A financial incentive of 500 Indian Rupee (6 United States Dollars) per month was proposed to be transferred directly to the registered beneficiaries’ validated bank accounts. We examined the reach, timing, amount of benefit receipt and the extent to which the benefit alleviated catastrophic costs (used as a proxy to measure the impact on permanent economic welfare as catastrophic cost is the level of cost that is likely to result in a permanent negative economic impact on households) by interviewing 1482 adult drug-susceptible TB patients from 16 districts of four states during 2019 to 2023, using the methods recommended by the World Health Organization for estimating household costs of TB nationally. We also estimated the potential amount of social support required to achieve a zero catastrophic cost target. At the end of treatment, 31–54% of study participants received the benefit. In all, 34–60% of TB patients experienced catastrophic costs using different estimation methods and the benefit helped 2% of study participants to remain below the catastrophic cost threshold. A uniform benefit amount of Indian Rupee 10 000 (127 United States Dollars) for 6 months of treatment could reduce the incidence of catastrophic costs by 43%. To improve the economic welfare of TB patients, levels of benefit need to be substantially increased, which will have considerable budgetary impact on the TB programme. Hence, a targeted rather than universal approach may be considered. To maximize impact, at least half of the revised amount should be given immediately after treatment registration.
Helmet wearing and related factors among electric personal mobility device users in Korea
Authors: Kim Eunbi , Kim Taehun , Park Sojeong , Park Sohyun , Park Juhwan , Lee Junhyeok , Lee Junhyung , Han Mi Ah 
Published: July 2024

Abstract
Using data from the 2022 Korea Community Health Survey (n = 13 320), this study investigated helmet use and related factors among Korean adults using personal mobility devices, without distinguishing between private and hired users. Among mobility device users, 32.1% responded that they always wore a helmet. The proportion of helmet use was 35.2% among men, 25.8% among women, 29.2% among those aged 19–44 years, 42.3% among those aged 45–64 years and 26.6% among those aged 65 years or older. Furthermore, those who drank less frequently and were physically active were more likely to wear helmets. Moreover, people who always wore a seat belt when driving a car or sitting in the rear seat and people who always wore a helmet when riding a motorcycle were more likely to wear a helmet while using electric personal mobility devices. Approximately one-third of users always wore a helmet. The helmet-wearing rate was related to general characteristics such as gender and education level, and to safety behaviors such as wearing a seat belt when driving a car, sitting in the rear seat of a car, or when riding a motorcycle. In addition to considering personal characteristics investigated in this study, the helmet-wearing rate should be improved through policies or systems at the national or regional levels.
Smartphones and video as security articulation infrastructures: evidencing Black Lives Matter
Authors: Andersen Rune Saugmann 
Published: July 2024

Abstract
Smartphone videos can co-constitute security reality. As smartphones spread in the 2010s, videos of deadly police violence against Black United States citizens became common, and over time these videos co-constituted anti-Black police violence as a security issue, which found its expression as Black Lives Matter (BLM). This article questions the role that smartphones and video play for BLM, and argues that security theory needs a better grasp of security articulation. Mapping the mediation of BLM's first decade, the article documents that smartphones are security articulation infrastructures as bystanders routinely rely on smartphone video to articulate security. The epistemic authority of video enables BLM videos to act as infrastructural gateways connecting established mass media to new vernacular media. Video mediation denies recognized figures of authority interpretive monopoly and enables non-elites to participate in constituting security reality, creating a room for non-elite Black Americans' articulation of insecurity. The article shows that still images and videos are different in this respect, and calls for security theory to take articulation formats and infrastructures seriously. When leaving ‘communication’ to other disciplines or enacting government responses as constitutive of (visual) security, scholarship risks overlooking the epistemic racism limiting security articulation in ‘old’ mass media, and risks making security theory complicit in epistemically silencing the voices of common, marginalized and racialized people.
Community pharmacists’ attitudes towards checking prescriptions: a cross-sectional survey
Authors: van Loon W Ellen , Lambert Maarten , Heringa Mette , Bouvy Marcel L , Taxis Katja 
Published: July 2024

Abstract

Background
The final prescription check is a mandatory but time-consuming process in Dutch community pharmacies. A safer dispensing process may have made the final prescription check obsolete.

Objective
To describe the final prescription check in Dutch community pharmacies and explore pharmacists’ attitudes towards changing this.

Methods
A cross-sectional survey among Dutch community pharmacists. The online questionnaire was based on literature and previous qualitative research, piloted in three pharmacies, and took 10 min to complete. Results were analysed descriptively.

Results
A total of 409 pharmacists participated. They saw the final prescription check as an important quality assurance of the dispensing process. Nevertheless, most pharmacists agreed that the final prescription check could be optimized as they thought that the time invested outweighed the benefits. Automation of the dispensing process, only checking selected high-risk prescriptions, and more in-process checks could reduce the need for an extensive final prescription check, rather than delegating the task to assistants. To implement changes, most pharmacists felt current dispensing guidelines needed to be adapted.

Conclusion
There was a widespread consensus that optimizing the final prescription check could enhance efficiency and allow more time for person-centred care. Most pharmacists expressed a preference for updated guidelines before implementing such changes.
Linking active rectal mucosa–attached microbiota to host immunity reveals its role in host–pathogenic STEC O157 interactions
Authors: Pan Zhe , Chen Yanhong , Zhou Mi , McAllister Tim A , Mcneilly Tom N , Guan Le Luo 
Published: July 2024

Abstract
The rectal–anal junction (RAJ) is the major colonization site of Shiga toxin–producing Escherichia coli (STEC) O157 in beef cattle, leading to transmission of this foodborne pathogen from farms to food chains. To date, there is limited understanding regarding whether the mucosa-attached microbiome has a profound impact on host–STEC interactions. In this study, the active RAJ mucosa–attached microbiota and its potential role in host immunity–STEC commensal interactions were investigated using RAJ mucosal biopsies collected from calves orally challenged with two STEC O157 strains with or without functional stx2a (stx2a+ or stx2a−). The results revealed that shifts of microbial diversity, topology, and assembly patterns were subjected to stx2a production post-challenge and Paeniclostridium and Gallibacterium were the keystone taxa for both microbial interactions and assembly. Additional mucosal transcriptome profiling showed stx2a−dependent host immune responses (i.e. B- and T-cell signaling and antigen processing and presentation) post-challenge. Further integrated analysis revealed that mucosa–attached beneficial microbes (i.e. Provotella, Faecalibacterium, and Dorea) interacted with host immune genes pre-challenge to maintain host homeostasis; however, opportunistic pathogenic microbes (i.e. Paeniclostridium) could interact with host immune genes after the STEC O157 colonization and interactions were stx2a−dependent. Furthermore, predicted bacterial functions involved in pathogen (O157 and Paeniclostridium) colonization and metabolism were related to host immunity. These findings suggest that during pathogen colonization, host–microbe interactions could shift from beneficial to opportunistic pathogenic bacteria driven and be dependent on the production of particular virulence factors, highlighting the potential regulatory role of mucosa–attached microbiota in affecting pathogen–commensal host interactions in calves with STEC O157 infection.
The impact of alcohol and illicit substance use on the pharmacokinetics of first-line TB drugs
Authors: Wijk Marie , Gausi Kamunkhwala , Malatesta Samantha , Weber Sarah E , Court Richard , Myers Bronwyn , Carney Tara , Parry Charles D H , Horsburgh C Robert , White Laura F , Wiesner Lubbe , Warren Robin M , Uren Caitlin , McIlleron Helen , Kloprogge Frank , Denti Paolo , Jacobson Karen R 
Published: July 2024

Abstract

Background
In South Africa, an estimated 11% of the population have high alcohol use, a major risk factor for TB. Alcohol and other substance use are also associated with poor treatment response, with a potential mechanism being altered TB drug pharmacokinetics.

Objectives
To investigate the impact of alcohol and illicit substance use on the pharmacokinetics of first-line TB drugs in participants with pulmonary TB.

Methods
We prospectively enrolled participants ≥15 years old, without HIV, and initiating drug-susceptible TB treatment in Worcester, South Africa. Alcohol use was measured via self-report and blood biomarkers. Other illicit substances were captured through a urine drug test. Plasma samples were drawn 1 month into treatment pre-dose, and 1.5, 3, 5 and 8 h post-dose. Non-linear mixed-effects modelling was used to describe the pharmacokinetics of rifampicin, isoniazid, pyrazinamide and ethambutol. Alcohol and drug use were tested as covariates.

Results
The study included 104 participants, of whom 70% were male, with a median age of 37 years (IQR 27–48). Alcohol use was high, with 42% and 28% of participants having moderate and high alcohol use, respectively. Rifampicin and isoniazid had slightly lower pharmacokinetics compared with previous reports, whereas pyrazinamide and ethambutol were consistent. No significant alcohol use effect was detected, other than 13% higher ethambutol clearance in participants with high alcohol use. Methaqualone use reduced rifampicin bioavailability by 19%.

Conclusion
No clinically relevant effect of alcohol use was observed on the pharmacokinetics of first-line TB drugs, suggesting that poor treatment outcome is unlikely due to pharmacokinetic alterations. That methaqualone reduced rifampicin means dose adjustment may be beneficial.
Bone matrix properties in adults with osteogenesis imperfecta are not adversely affected by setrusumab—a sclerostin neutralizing antibody
Authors: Rummler Maximilian , Schemenz Victoria , McCluskey Samantha , Davydok Anton , Rauch Frank , Glorieux Francis H , Harrington Matthew J , Wagermaier Wolfgang , Willie Bettina M , Zimmermann Elizabeth A 
Published: July 2024

Abstract
Osteogenesis imperfecta (OI) is a skeletal dysplasia characterized by low bone mass and frequent fractures. Children with OI are commonly treated with bisphosphonates to reduce fracture rate, but treatment options for adults are limited. In the Phase 2b ASTEROID trial, setrusumab (a sclerostin neutralizing antibody, SclAb) improved bone density and strength in adults with type I, III, and IV OI. Here, we investigate bone matrix material properties in tetracycline-labeled trans iliac biopsies from 3 groups: (1) control: individuals with no metabolic bone disease, (2) OI: individuals with OI, (3) SclAb-OI: individuals with OI after 6 mo of setrusumab treatment (as part of the ASTEROID trial). In addition to bone histomorphometry, bone mineral and matrix properties were evaluated with nanoindentation, Raman spectroscopy, second harmonic generation imaging, quantitative backscatter electron imaging, and small-angle X-ray scattering. Spatial locations of fluorochrome labels were identified to differentiate inter-label bone of the same tissue age and intra-cortical bone. No difference in collagen orientation was found between the groups. The bone mineral density distribution and analysis of Raman spectra indicate that OI groups have greater mean mineralization, greater relative mineral content, and lower crystallinity than the control group, which was not altered by SclAb treatment. Finally, a lower modulus and hardness were measured in the inter-label bone of the OI-SclAb group compared to the OI group. Previous studies suggest that even though bone from OI has a higher mineral content, the extracellular matrix (ECM) has comparable mechanical properties. Therefore, fragility in OI may stem from contributions from other yet unexplored aspects of bone organization at higher length scales. We conclude that SclAb treatment leads to increased bone mass while not adversely affecting bone matrix properties in individuals with OI.
Temperature acclimation and response to acute thermal stress in the adults of the snow crab Chionoecetes opilio Fabricius, 1788 (Decapoda: Brachyura: Oregoniidae)
Authors: Thesslund Tina , Wittmann Astrid C , Timmerhaus Gerrit , Berg Ragnhild S , Hansen Marianne H S , Siikavuopio Sten , Evensen Tor H , Andersen Øivind 
Published: July 2024

ABSTRACT
Ectothermic marine animals vary widely in their tolerance to temperature changes, and polar stenothermal species seem to have poor ability to compensate for a rise in water temperature. The sub-Arctic snow crab (Chionoecetes opilioFabricius, 1788) lives in the northwestern Atlantic Ocean and northern Pacific Ocean at temperatures ranging from about −1.5 °C to 4 °C. Since the metabolic costs overtake caloric intake above 7 °C, the snow crab appears to be energetically restricted to cold water. We investigated thermal stress responses in adult male crabs exposed to a sudden temperature increase to 9.5 °C for 24 hr after four weeks of acclimation at 2.5 °C or 5.5 °C. Heart-rate loggers implanted in a limited number of crabs showed 60% increase in cardiac activity during the thermal stress. Surplus oxygen supply in all crabs was inferred by the low hemolymph lactate and unchanged glucose levels, but only the crabs acclimated at 5.5 °C were still active at the elevated temperature. Low heat shock and oxidative stress responses were suggested by the missing upregulation of the genes encoding four heat shock proteins (Hsp70a, Hsc71, Hsp90a2, Hsp60) and the antioxidative enzymes superoxide dismutase and catalase. The trend towards inverse temperature-dependent on the expression of the hsp genes may be related to increased protein damage at low temperatures, or possible trade-offs between costs and benefits of producing heat shock proteins at elevated temperature. Although adult snow crabs seem to be able to cope with short-term heat stress, the tolerance to chronic elevated temperatures should be further examined using a larger number of individuals.
Effects of combining soil-applied insecticide and Bt corn for integrated pest management and resistance management of western corn rootworm (Coleoptera: Chrysomelidae)
Authors: McCulloch John B , Gassmann Aaron J 
Published: July 2024

Abstract
The western corn rootworm, (Diabrotica virgifera virgifera LeConte, Coleoptera: Chrysomelidae), is a serious pest of corn (Zea mays Linnaeus, Cyperales: Poaceae) in the midwestern United States. Management practices for corn rootworm larvae include crop rotation, transgenic corn producing insecticidal toxins from the bacterium Bacillus thuringiensis Berliner (Bacillales: Bacillaceae) (Bt), and soil-applied insecticides. The extent to which combining soil-applied insecticide with Bt corn would be beneficial from the perspective of insect resistance management (IRM) or integrated pest management (IPM) remains uncertain. We conducted a 3-yr field study to characterize the implications of combining a soil-applied insecticide and Bt corn for IRM and IPM of western corn rootworm. Experimental treatments were Bt corn, a soil-applied insecticide, the combination of these factors, and an experimental control in which both factors were absent. Data were collected on root injury to corn by rootworm, survival to adulthood, adult size, and emergence time for western corn rootworm. We found that mortality caused by the soil-applied insecticide was insufficient to delay resistance to Bt corn. While combining Bt corn and a soil-applied insecticide may provide a short-term economic benefit, additional research is needed to determine appropriate economic thresholds for combining these tactics. Additionally, combining a soil-applied insecticide and Bt corn would not be sustainable over multiple growing seasons because of its potential to rapidly select for Bt resistance. In general, a more sustainable IRM strategy for rootworm management would include using crop rotation and alternating between non-Bt corn with soil-applied insecticide and Bt corn without soil-applied insecticide.
Toxicity of cockroach gel baits to the oothecal parasitoid Aprostocetus hagenowii (Hymenoptera: Eulophidae) and implications for cockroach integrated pest management
Authors: Smith Chelsea M , Griffin Madeline P , Fadamiro Henry Y , Appel Arthur G 
Published: July 2024

Abstract
Aprostocetus hagenowii (Ratzeburg) is a parasitoid wasp that parasitizes the oothecae of peridomestic pest cockroaches. A. hagenowii has been used in integrated pest management (IPM) programs for cockroach control but little is known about how this parasitoid responds to the insecticides commonly used for cockroach management. Five insecticidal gel bait products containing indoxacarb, clothianidin, fipronil, dinotefuran, or abamectin B1 were tested for their toxicity towards A. hagenowii and the American cockroach, Periplaneta americana (L.; Blattodea: Blattidae), a host of A. hagenowii and a common pest. All baits were tested as fresh and 1-d aged deposits. Indoxacarb was the only active ingredient that did not cause significant (P &lt; 0.05) A. hagenowii mortality compared to the control in both the fresh and aged gel experiments (Median survival time [MST]s: 168 h fresh, 72 h aged). Clothianidin caused the lowest A. hagenowii MSTs across experiments (24 h, fresh and aged). All baits caused significant P. americana mortality as fresh and 1-d aged deposits (P &lt; 0.05). Indoxacarb appears most compatible with A. hagenowii in cockroach IPM.
In vivo and in silico elucidation of possible potential and mechanisms involved in the analgesic action of ethanolic extract of Lavandula Stoechas
Authors: Nazir Muhammad Muzammil , Inam Sana , Ijaz Muhammad Umar , Zafar Nimrah , Yeni Derya Karatas , Asad Farkhanda , Farzeen Iqra , Ashraf Asma 
Published: July 2024

Abstract

Objectives
Our research focused on plant’s ethanolic extract Lavandula stoechas flower part to investigate the potential analgesic effects and possible pathways involvements.

Methods
Four experimental tests were performed on Swiss albino mice with five animals in each group at different doses (50, 100, and 200mg/kg); formalin test, tail-flick test, acetic acid-induced writhing, and hot-plate test. The opioidergic, noradrenergic, cholinergic, and K channel blockers in the analgesic actions were also carried out for the potential route involvement.

Key finding
The percentage inhibition for abdominal writhing’s and formalin activity showed a dose-dependent manner for early and late phases reducing abdominal writhing’s and time period of licking, respectively. Tail immersion and hot-plate test demonstrated a substantial and dose-dependent increase in the latency time and time period of paw liking and jumping response respectively. GC–MS showed the abundantly present compounds were octadecatrienoic acid (34.35%), n-hexadecanoic acid (12.98%). In silico analyses have revealed three compounds that had good interactions with 6y3c receptor proteins, demonstrating strong binding affinities and satisfying docking parameters.

Conclusions
Overall, these studies showed that ethanolic extract of L. stoechas is an important medicinal plant, with both central and peripheral antinociceptive and analgesic activities supporting its traditional use for therapeutic purposes.
Anoectochilus roxburghii polysaccharide reduces D-GalN/LPS-induced acute liver injury by regulating the activation of multiple inflammasomes
Authors: Yan Yulu , Ye Xiqi , Huang Chunqing , Wu Junjun , Liu Yunbiao , Zheng Pingping , Shen Congqi , Bai Zhaofang , Tingming Shen 
Published: July 2024

Abstract

Background
Acute liver injury (ALI) is a serious syndrome with a high mortality rate due to viral infection, toxic exposure, and autoimmunity, and its severity can range from mildly elevated liver enzymes to severe liver failure. Activation of the nod-like receptor pyrin domain-containing 3 (NLRP3) inflammasome is closely associated with the development of ALI, and the search for an inhibitor targeting this pathway may be a novel therapeutic option. Anoectochilus roxburghii polysaccharide (ARP) is a biologically active ingredient extracted from Anoectochilus roxburghii with immunomodulatory, antioxidant, and anti-inflammatory bioactivities and pharmacological effects. In this study, we focused on D-galactosamine (D-GalN)/lipopolysaccharide (LPS)-induced acute liver injury by ARP through inhibition of NLRP3 inflammasome.

Methods
An inflammasome activation model was established in bone marrow-derived macrophages (BMDMs) to investigate the effects of ARP on caspase-1 cleavage, IL-1β secretion, and ASC oligomerization in inflammasomes under different agonists. We used the D-GalN/LPS-induced acute liver injury model in mice, intraperitoneally injected ARP or MCC950, and collected liver tissues, serum, and intraperitoneal lavage fluid for pathological and biochemical indexes.

Results
ARP effectively inhibited the activation of the NLRP3 inflammasome and had an inhibitory effect on non-classical NLRP3, AIM2, and NLRC4 inflammasomes. It also effectively inhibited the oligomerization of apoptosis-associated speck-like protein (ASC) from a variety of inflammatory vesicles. Meanwhile, ARP has good therapeutic effects on acute liver injury induced by D-GaIN/LPS.

Conclusion
The inhibitory effect of ARP on a wide range of inflammasomes, as well as its excellent protection against acute liver injury, suggests that ARP may be a candidate for acute liver injury.
The turnover of ABI5 by scaffold proteins to attenuate ABA signaling
Authors: Pollo-Rodríguez Fátima , Sánchez-Vicente Inmaculada , Lorenzo Oscar 
Published: July 2024

Abstract
This article comments on:
Li Z, Zhang D, Liang X, Liang J. 2024. Receptor for Activated C Kinase 1 counteracts ABSCISIC ACID INSENSITIVE5-mediated inhibition of seed germination and post-germinative growth in Arabidopsis. Journal of Experimental Botany 75, 3932–3945.