bluuebunny/crossref_metadata_2025_split huggingface dataset:
Benmelstobart, anlotinib and chemotherapy in extensive-stage small-cell lung cancer: a randomized phase 3 trial
Authors: Cheng Ying , Chen Jianhua , Zhang Wei , Xie Chao , Hu Qun , Zhou Ningning , Huang Chun , Wei Shihong , Sun Hong , Li Xingya , Yu Yan , Lai Jinhuo , Yang Huaping , Fang Haohui , Chen Hualin , Zhang Peng , Gu Kangsheng , Wang Qiming , Shi Jianhua , Yi Tienan , Xu Xingxiang , Ye Xianwei , Wang Daqing , Xie Conghua , Liu Chunling , Zheng Yulong , Lin Daren , Zhuang Wu , Lu Ping , Yu Guohua , Li Jinzhang , Gu Yuhai , Li Baolan , Wu Rong , Jiang Ou , Wang Zaiyi , Wu Guowu , Lin Haifeng , Zhong Diansheng , Xu Yanhua , Shu Yongqian , Wu Di , Chen Xingwu , Wang Jie , Wang Minghui , Yang Runxiang 
Published: July 2024

AbstractImmunochemotherapy is the first-line standard for extensive-stage small-cell lung cancer (ES-SCLC). Combining the regimen with anti-angiogenesis may improve efficacy. ETER701 was a multicenter, double-blind, randomized, placebo-controlled phase 3 trial that investigated the efficacy and safety of benmelstobart (a novel programmed death-ligand 1 (PD-L1) inhibitor) with anlotinib (a multi-target anti-angiogenic small molecule) and standard chemotherapy in treatment-naive ES-SCLC. The ETER701 trial assessed two primary endpoints: Independent Review Committee-assessed progression-free survival per RECIST 1.1 and overall survival (OS). Here the prespecified final progression-free survival and interim OS analysis is reported. Patients randomly received benmelstobart and anlotinib plus etoposide/carboplatin (EC; n = 246), placebo and anlotinib plus EC (n = 245) or double placebo plus EC (‘EC alone’; n = 247), followed by matching maintenance therapy. Compared with EC alone, median OS was prolonged with benmelstobart and anlotinib plus EC (19.3 versus 11.9 months; hazard ratio 0.61; P = 0.0002), while improvement of OS was not statistically significant with anlotinib plus EC (13.3 versus 11.9 months; hazard ratio 0.86; P = 0.1723). The incidence of grade 3 or higher treatment-related adverse events was 93.1%, 94.3% and 87.0% in the benmelstobart and anlotinib plus EC, anlotinib plus EC, and EC alone groups, respectively. This study of immunochemotherapy plus multi-target anti-angiogenesis as first-line treatment achieved a median OS greater than recorded in prior randomized studies in patients with ES-SCLC. The safety profile was assessed as tolerable and manageable. Our findings suggest that the addition of anti-angiogenesis therapy to immunochemotherapy may represent an efficacious and safe approach to the management of ES-SCLC. ClinicalTrials.gov identifier: NCT04234607.
A near complete genome assembly of the East Friesian sheep genome
Authors: You Xiaoxiao , Fang Qinyuan , Chen Chunhai , Cao Junwei , Fu Shaoyin , Zhang Teng , Wang Shenyuan , He Xiaolong , He Jiangfeng , Zhou Yang , Wang Biao , Wang Liwei , Wang Zheng , Sun Tianhao , Yang Xukui , Te Rigele , Jian Jianbo , Zhou Huanmin , Dai Yanfeng , Liu Yongbin 
Published: July 2024

AbstractAdvancements in sequencing have enabled the assembly of numerous sheep genomes, significantly advancing our understanding of the link between genetic variation and phenotypic traits. However, the genome of East Friesian sheep (Ostfriesisches Milchschaf), a key high-yield milk breed, remains to be fully assembled. Here, we constructed a near-complete and gap-free East Friesian genome assembly using PacBio HiFi, ultra-long ONT and Hi-C sequencing. The resulting genome assembly spans approximately 2.96 Gb, with a contig N50 length of 104.1 Mb and only 164 unplaced sequences. Remarkably, our assembly has captured 41 telomeres and 24 centromeres. The assembled sequence is of high quality on completeness (BUSCO score: 97.1%) and correctness (QV: 69.1). In addition, a total of 24,580 protein-coding genes were predicted, of which 97.2% (23,891) carried at least one conserved functional domain. Collectively, this assembly provides not only a near T2T gap-free genome, but also provides a valuable genetic resource for comparative genome studies of sheep and will serve as an important tool for the sheep research community.
Second-harmonic generation tensors from high-throughput density-functional perturbation theory
Authors: Trinquet Victor , Naccarato Francesco , Brunin Guillaume , Petretto Guido , Wirtz Ludger , Hautier Geoffroy , Rignanese Gian-Marco 
Published: July 2024

AbstractOptical materials play a key role in enabling modern optoelectronic technologies in a wide variety of domains such as the medical or the energy sector. Among them, nonlinear optical crystals are of primary importance to achieve a broader range of electromagnetic waves in the devices. However, numerous and contradicting requirements significantly limit the discovery of new potential candidates, which, in turn, hinders the technological development. In the present work, the static nonlinear susceptibility and dielectric tensor are computed via density-functional perturbation theory for a set of 579 inorganic semiconductors. The computational methodology is discussed and the provided database is described with respect to both its data distribution and its format. Several comparisons with both experimental and results from literature allow to confirm the reliability of our data. The aim of this work is to provide a relevant dataset to foster the identification of promising nonlinear optical crystals in order to motivate their subsequent experimental investigation.
Histopathology imaging and clinical data including remission status in pediatric inflammatory bowel disease
Authors: Martin-King Chloe , Nael Ali , Ehwerhemuepha Louis , Calvo Blake , Gates Quinn , Janchoi Jamie , Ornelas Elisa , Perez Melissa , Venderby Andrea , Miklavcic John , Chang Peter , Sassoon Aaron , Grant Kenneth 
Published: July 2024

AbstractThe incidence of inflammatory bowel disease (IBD) is increasing annually. Children with IBD often suffer significant morbidity due to physical and emotional effects of the disease and treatment. Corticosteroids, often a component of therapy, carry undesirable side effects with long term use. Steroid-free remission has become a standard for care-quality improvement. Anticipating therapeutic outcomes is difficult, with treatments often leveraged in a trial-and-error fashion. Artificial intelligence (AI) has demonstrated success in medical imaging classification tasks. Predicting patients who will attain remission will help inform treatment decisions. The provided dataset comprises 951 tissue section scans (167 whole-slides) obtained from 18 pediatric IBD patients. Patient level structured data include IBD diagnosis, 12- and 52-week steroid use and name, and remission status. Each slide is labelled with biopsy site and normal or abnormal classification per the surgical pathology report. Each tissue section scan from an abnormal slide is further classified by an experienced pathologist. Researchers utilizing this dataset may select from the provided outcomes or add labels and annotations from their own institutions.
Pressurized liquid extraction followed by high-performance liquid chromatography for determination of beta-ecdysone extracted from Pfaffia glomerata (Spreng.) Pedersen
Authors: de Oliveira Charlini Balastreri Dorta , Filho Lúcio Cardozo , de Mello João Carlos Palazzo , Júnior Osvaldo Valarini , Calaça Giselle Nathaly , Rocha Stella Alonso , Sato Francielle , Sakai Otávio Akira 
Published: July 2024

AbstractPfaffia glomerata (Spreng.) Pedersen has among its main bioactive compounds saponins, with the phytoestroid β-ecdysone as its chemical marker. In this study, pressurized liquid extraction (PLE), a green extraction technique used to obtain bioactive compounds from plants, was employed to extract beta-ecdysone from P. glomerata leaves, stems, and roots. The 22 factorial design was used with the variables temperature (333 K and 353 K) and flow rate (1.5 and 2 mL min−1), pressure (300 Bar), time (60 min), and solvent [ethanol and distilled water (70:30 (v/v)] were kept constant for all parts of the plant. The results of experimental responses demonstrated that the factors temperature and flow rate significantly interfere with the yields of leaf (0.499%), root (0.65%) and stem (0.764%) extracts. The latter presented presents the highest yield compared to the other parts of the plant. HPLC results showed the presence of beta-ecdysone in all parts of the plant with concentrations of β-ecdysone 86.82, 76.53 and 195.86 mg L−1 to leaf, root and stem, respectively. FT Raman results exhibited typical peaks of beta-ecdysone, such as 3310 cm−1, 1654 cm−1, and 1073 cm−1 for all plant parts. Another interesting result was the presence of the peak at 1460 cm−1 in the PLE root extract can be associated with selenium. This foundational knowledge confirms that the PLE extraction process was efficient in obtaining the chemical marker of Pfaffia glomerata in all plant parts.
Impact of the quality and diversity of reference products on creative activities in online communities
Authors: Sato Keisuke , Yang Kunhao , Ueda Kazuhiro 
Published: July 2024

AbstractIn creativity research, an important unresolved issue relates to identifying the kind of information an individual should be exposed to in order to be highly creative. We aimed to gain insights into this issue; we collected and statistically analyzed three datasets related to three large online communities (namely Cities: Skylines, SCP-wiki, and Archive of Our Own) engaged in mod development and novel writing to examine whether the quality and diversity of other people’s products referred to have a positive effect on product generation. Our analysis revealed the following three findings: (1) the quality diversity of reference products generated by others has the most positive impact on the quality of generated products when it is neither high nor low, (2) the content diversity of reference products generated by others has a negative impact on the quality of generated products, and (3) the quality of reference products generated by others has a negative impact on the quality of generated products when it is extremely high. We conclude by discussing the implications of the findings for creativity research.
BOMS: blockchain-enabled organ matching system
Authors: Igboanusi Ikechi Saviour , Nnadiekwe Chigozie Athanasius , Ogbede Joseph Uche , Kim Dong-Seong , Lensky Artem 
Published: July 2024

AbstractThis work proposes a Blockchain-enabled Organ Matching System (BOMS) designed to manage the process of matching, storing, and sharing information. Biological factors are incorporated into matching and the cross-matching process is implemented into the smart contracts. Privacy is guaranteed by using patient-associated blockchain addresses, without transmitting or using patient personal records in the matching process. The matching algorithm implemented as a smart contract is verifiable by any party. Clinical records, process updates, and matching results are also stored on the blockchain, providing tamper-resistance of recipient’s records and the recipients’ waiting queue. The system also is capable of handling cases in which there is a donor without an immediate compatible recipient. The system is implemented on the Ethereum blockchain and several scenarios were tested. The performance of the proposed system is compared to other existing organ donation systems, and ours outperformed any existing organ matching system built on blockchain. BOMS is tested to ascertain its compatibility with public, private, and consortium blockchain networks, checks for security vulnerabilities and cross-matching efficiency. The implementation codes are available online.
Turnover intention and its related factors of clinical research coordinator in Hunan, China: a cross-sectional study
Authors: Li Juan , Li JinHua , She ZhengDi , Guo LiWen , Gu ShanZhi , Lu Wen 
Published: July 2024

AbstractTo explore the related factors of turnover intention in clinical research coordinators (CRCs) and assess the mediating effects of professional identity on the association between job burnout and turnover intention. In China, CRC has become increasingly common among clinical trial teams in recent years. However, limited published research focused on the status of turnover intention in CRCs. We invited all the 220 CRCs currently working at Hunan Cancer Hospital located in Changsha city in the central south of China from March to June 2018. Participants were asked to complete structured questionnaires regarding basic demographic information, job burnout, professional identity and turnover intention. A total of 202 participants were included in this study, with a response rate of 91.82%. The main reason for turnover intention among CRCs was human resources, followed by communications, management and material resources (per item score in each dimension: 2.14 vs. 2.43 vs. 2.65 vs. 2.83). All the correlations among job burnout, professional identity and turnover intention were statistically significant, with coefficients ranging from −0.197 to 0.615. Multiple liner regression analysis showed that older age, longer workhours per week, and lower level of professional identity were associated with the prevalence of turnover intention among CRCs. Besides, the association between job burnout and turnover intention was fully mediated by professional identity. This study revealed the status and causes of turnover intention among Chinese CRCs. Effective measures on decreasing working time and improving professional identity should be taken in order to reduce CRCs’ turnover intention.
An optical photothermal infrared investigation of lymph nodal metastases of oral squamous cell carcinoma
Authors: Al Jedani Safaa , Lima Cassio , Smith Caroline I. , Gunning Philip J. , Shaw Richard J. , Barrett Steve D. , Triantafyllou Asterios , Risk Janet M. , Goodacre Royston , Weightman Peter 
Published: July 2024

AbstractIn this study, optical photothermal infrared (O-PTIR) spectroscopy combined with machine learning algorithms were used to evaluate 46 tissue cores of surgically resected cervical lymph nodes, some of which harboured oral squamous cell carcinoma nodal metastasis. The ratios obtained between O-PTIR chemical images at 1252 cm−1and 1285 cm−1were able to reveal morphological details from tissue samples that are comparable to the information achieved by a pathologist’s interpretation of optical microscopy of haematoxylin and eosin (H&E) stained samples. Additionally, when used as input data for a hybrid convolutional neural network (CNN) and random forest (RF) analyses, these yielded sensitivities, specificities and precision of 98.6 ± 0.3%, 92 ± 4% and 94 ± 5%, respectively, and an area under receiver operator characteristic (AUC) of 94 ± 2%. Our findings show the potential of O-PTIR technology as a tool to study cancer on tissue samples.
Nuclear actin assembly is an integral part of decidualization in human endometrial stromal cells
Authors: Tamura Isao , Miyamoto Kei , Hatanaka Chiharu , Shiroshita Amon , Fujimura Taishi , Shirafuta Yuichiro , Mihara Yumiko , Maekawa Ryo , Taketani Toshiaki , Sato Shun , Matsumoto Kazuya , Tamura Hiroshi , Sugino Norihiro 
Published: July 2024

AbstractDecidualization of the human endometrium is critical for establishing pregnancy and is entailed by differentiation of endometrial stromal cells (ESCs) into decidual cells. During decidualization, the actin cytoskeleton is dynamically reorganized for the ESCs’ morphological and functional changes. Although actin dynamically alters its polymerized state upon external stimuli not only in the cytoplasm, but also in the nucleus, nuclear actin dynamics during decidualization have not been elucidated. Here, we show that nuclear actin was specifically assembled during decidualization of human ESCs. This decidualization-specific formation of nuclear actin filaments was disassembled following the withdrawal of the decidualization stimulus, suggesting its reversible process. Mechanistically, RNA-seq analyses revealed that the forced disassembly of nuclear actin resulted in the suppression of decidualization, accompanied with the abnormal upregulation of cell proliferation genes, leading to incomplete cell cycle arrest. CCAAT/enhancer-binding protein beta (C/EBPβ), an important regulator for decidualization, was responsible for downregulation of the nuclear actin exporter, thus accelerating nuclear actin accumulation and its assembly for decidualization. Taken together, we demonstrate that decidualization-specific nuclear actin assembly induces cell cycle arrest for establishing the decidualized state of ESCs. We propose that not only the cytoplasmic actin, but also nuclear actin dynamics profoundly affect decidualization process in humans for ensuring pregnancy.
A RsrC-RsrA-RsrB transcriptional circuit positively regulates polysaccharide-degrading enzyme biosynthesis and development in Penicillium oxalicum
Authors: Ning Yuan-Ni , Liang Xue , Shen Xin , Tian Di , Li Wen-Tong , Luo Xue-Mei , Feng Jia-Xun , Zhao Shuai 
Published: July 2024

AbstractFilamentous fungi produce polysaccharide-degrading enzymes, which is controlled by poorly understood transcriptional circuits. Here we show that a circuit comprising RsrC-RsrA-RsrB (Rsr: production of raw-starch-degrading enzyme regulator) that positively regulates production of raw starch-degrading enzymes in Penicillium oxalicum. Transcription factor (TF) RsrA is essential for biosynthesis of raw starch-degrading enzymes. RsrB and RsrC containing Zn2Cys6- and C2H2-zinc finger domains, act downstream and upstream of RsrA, respectively. RsrA activates rsrB transcription, and three nucleotides (G-286, G-287 and G-292) of rsrB promoter region are required for RsrA, in terms of TF, for binding. RsrB165−271 binds to DNA sequence 5’-TCGATCAGGCACGCC-3’ in the promoter region of the gene encoding key raw-starch-degrading enzyme PoxGA15A. RsrC specifically binds rsrA promoter, but not amylase genes, to positively regulate the expression of rsrA and the production of raw starch-degrading enzymes. These findings expand complex regulatory network of fungal raw starch-degrading enzyme biosynthesis.
A first-in-class selective inhibitor of EGFR and PI3K offers a single-molecule approach to targeting adaptive resistance
Authors: Whitehead Christopher E. , Ziemke Elizabeth K. , Frankowski-McGregor Christy L. , Mumby Rachel A. , Chung June , Li Jinju , Osher Nathaniel , Coker Oluwadara , Baladandayuthapani Veerabhadran , Kopetz Scott , Sebolt-Leopold Judith S. 
Published: July 2024

AbstractDespite tremendous progress in precision oncology, adaptive resistance mechanisms limit the long-term effectiveness of molecularly targeted agents. Here we evaluated the pharmacological profile of MTX-531 that was computationally designed to selectively target two key resistance drivers, epidermal growth factor receptor and phosphatidylinositol 3-OH kinase (PI3K). MTX-531 exhibits low-nanomolar potency against both targets with a high degree of specificity predicted by cocrystal structural analyses. MTX-531 monotherapy uniformly resulted in tumor regressions of squamous head and neck patient-derived xenograft (PDX) models. The combination of MTX-531 with mitogen-activated protein kinase kinase or KRAS-G12C inhibitors led to durable regressions of BRAF-mutant or KRAS-mutant colorectal cancer PDX models, resulting in striking increases in median survival. MTX-531 is exceptionally well tolerated in mice and uniquely does not lead to the hyperglycemia commonly seen with PI3K inhibitors. Here, we show that MTX-531 acts as a weak agonist of peroxisome proliferator-activated receptor-γ, an attribute that likely mitigates hyperglycemia induced by PI3K inhibition. This unique feature of MTX-531 confers a favorable therapeutic index not typically seen with PI3K inhibitors.
Melanometry for objective evaluation of skin pigmentation in pulse oximetry studies
Authors: Vasudevan Sandhya , Vogt William C. , Weininger Sandy , Pfefer T. Joshua 
Published: July 2024

AbstractPulse oximetry enables real-time, noninvasive monitoring of arterial blood oxygen levels. However, results can vary with skin color, thus detecting disparities during clinical validation studies requires an accurate measure of skin pigmentation. Recent clinical studies have used subjective methods such as self-reported color, race/ethnicity to categorize skin. Melanometers based on optical reflectance may offer a more effective, objective approach to assess pigmentation. Here, we review melanometry approaches and assess evidence supporting their use as clinical research tools. We compare performance data, including repeatability, robustness to confounders, and compare devices to each other, to subjective methods, and high-quality references. Finally, we propose best practices for evaluating melanometers and discuss alternate optical approaches that may improve accuracy. Whilst evidence indicates that melanometers can provide superior performance to subjective approaches, we encourage additional research and standardization efforts, as these are needed to ensure consistent and reliable results in clinical studies.
Tumor location is associated with mood dysfunction in patients with diffuse glioma
Authors: van Genderen Maisa N. G. , Belgers Vera , Niers Johanna M. , Douw Linda , Röttgering Jantine G. , Gorter Maxine , Blom Marieke E. C. , Barkhof Frederik , Klein Martin , Eijgelaar Roelant S. , De Witt Hamer Philip C. 
Published: July 2024

AbstractGliomas are primary brain tumors that can cause neuropsychiatric symptoms, including severe depressive symptoms (SDS; in 14%) and an absence of depressive symptoms (ADS; in 29%), determined by Center for Epidemiologic Studies Depression (CES-D) scores. We examined the association between both SDS and ADS and brain tumor location in 201 patients with diffuse glioma before surgery. Tumors and white matter disconnectomes did not relate to CES-D using sparse canonical correlation analysis. SDS were associated with tumors in the right corticospinal tract, fornix, and inferior fronto-occipital fasciculus and the left uncinate fasciculus, whereas ADS was associated with tumors in the left uncinate fasciculus and first segment of the superior longitudinal fasciculus and the right temporal cingulum and thalamus using Bayesian regression analyses. ADS occurs even more frequently in patients with diffuse glioma than does SDS, which is explained partly by tumor location. This research aids the understanding of gliomas and mood dysfunction in general.