bluuebunny/crossref_metadata_2025_split huggingface dataset:
Life‐threatening delayed hyperhemolytic transfusion reaction in a patient with sickle cell disease: effective treatment with eculizumab followed by rituximab
Authors: Boonyasampant Mark , Weitz Ilene C. , Kay Brian , Boonchalermvichian Chaiyaporn , Liebman Howard A. , Shulman Ira A. 
Published: May 2015

BACKGROUNDHyperhemolysis in sickle cell disease is a rare and potentially life‐threatening complication of transfusion.STUDY DESIGN AND METHODSIn this article we report a case of delayed hemolytic transfusion reaction with resultant hyperhemolysis triggered by an anti‐IH autoantibody with alloantibody behavior.RESULTSThe anti‐IH was reactive at room temperature as well as 37°C, but only weakly reactive with autologous red blood cells. Initial cold agglutinin titer was 512. The profound, life‐threatening, intravascular hemolysis was rapidly and dramatically reduced with the Complement 5 (C5) inhibitory antibody, eculizumab. The auto/allo cold agglutinin was subsequently suppressed with rituximab treatment.CONCLUSIONSEculizumab, a potent C5 inhibitory antibody, can be a rapid and effective therapy for hyperhemolytic transfusion reactions when given in a sufficient dose to fully block the activation of complement C5.
Sequencing of the ART4 gene in sub‐Saharan cohorts reveals ethnic differences and two new DO alleles: DO*B‐Ile5Thr and DO*B‐Trp266Arg
Authors: Durousseau de Coulgeans Cécile , Chiaroni Jacques , Bailly Pascal , Chapel‐Fernandes Sylvie 
Published: May 2015

BACKGROUNDGiven the high heterogeneity of sub‐Saharan populations especially between nonpygmoids and pygmoids, differences are expected during investigation of the DO/ART4 gene.STUDY DESIGN AND METHODSUsing genomic DNA extracted from blood samples collected from 77 Tswa pygmoids and 39 Teke and seven San nonpygmoids, DO coding regions were amplified and sequenced. A tetra‐primer amplification refractory mutation system‐polymerase chain reaction method was developed to specifically detect the DO*B‐SH‐Gln149Lys variant. Membrane expression of newly identified variant alleles in K562‐transduced cells was studied by flow cytometry.RESULTSExtensive polymorphism was confirmed in Teke or San nonpygmoids and Tswa pygmoids with, respectively, 12, zero, and 24 DO*A; 54, 10, and 115 DO*B or DO*B‐WL; five, zero, and 14 DO*HY; and six DO*JO alleles in Teke only. The DO*B‐SH‐Gln149Lys variant was observed as the third most frequent after the DO*HY and DO*JO alleles. Two novel DO*B alleles were identified in the San samples, that is, DO*B‐Ile5Thr and DO*B‐Trp266Arg. Study of K562‐transduced cells showed that compared to the DO*B allele, DO*B‐Ile5Thr was expressed more strongly while DO*B‐Trp266Arg variant was expressed to a lesser extent and was not recognized by MIMA‐123 monoclonal antibodies.CONCLUSIONSequencing analysis showed more allelic combinations in nonpygmoids than in pygmoids with high frequencies of DO*HY, DO*JO, and DO*B‐SH‐Gln149Lys variant alleles. This finding underlines the importance of including DO*HY and DO*JO single‐nucleotide polymorphisms in genotyping tests to improve transfusion safety. Characterization of two novel DO*B alleles highlights the value of testing selected ethnic groups in understanding DO allele diversity.
Quality of red blood cells washed using a second wash sequence on an automated cell processor
Authors: Hansen Adele L. , Turner Tracey R. , Kurach Jayme D.R. , Acker Jason P. 
Published: May 2015

BACKGROUNDWashed red blood cells (RBCs) are indicated for immunoglobulin (Ig)A‐deficient recipients when RBCs from IgA‐deficient donors are not available. Canadian Blood Services recently began using the automated ACP 215 cell processor (Haemonetics Corporation) for RBC washing, and its suitability to produce IgA‐deficient RBCs was investigated.STUDY DESIGN AND METHODSRBCs produced from whole blood donations by the buffy coat (BC) and whole blood filtration (WBF) methods were washed using the ACP 215 or the COBE 2991 cell processors and IgA and total protein levels were assessed. A double‐wash procedure using the ACP 215 was developed, tested, and validated by assessing hemolysis, hematocrit, recovery, and other in vitro quality variables in RBCs stored after washing, with and without irradiation.RESULTSA single wash using the ACP 215 did not meet Canadian Standards Association recommendations for washing with more than 2 L of solution and could not consistently reduce IgA to levels suitable for IgA‐deficient recipients (24/26 BC RBCs and 0/9 WBF RBCs had IgA levels < 0.05 mg/dL). Using a second wash sequence, all BC and WBF units were washed with more than 2 L and had levels of IgA of less than 0.05 mg/dL. During 7 days' postwash storage, with and without irradiation, double‐washed RBCs met quality control criteria, except for the failure of one RBC unit for inadequate (69%) postwash recovery.CONCLUSIONUsing the ACP 215, a double‐wash procedure for the production of components for IgA‐deficient recipients from either BC or WBF RBCs was developed and validated.
Different dynamin blockers interfere with distinct phases of synaptic endocytosis during stimulation in motoneurones
Authors: Linares‐Clemente Pedro , Rozas José L. , Mircheski Josif , García‐Junco‐Clemente Pablo , Martínez‐López José A. , Nieto‐González José L. , Vázquez M. Eugenio , Pintado C. Oscar , Fernández‐Chacón Rafael 
Published: May 2015

Key points
Neurotransmitter release requires a tight coupling between synaptic vesicle exocytosis and endocytosis with dynamin being a key protein in that process.
We used imaging techniques to examine the time course of endocytosis at mouse motor nerve terminals expressing synaptopHluorin, a genetically encoded reporter of the synaptic vesicle cycle.
We separated two sequential phases of endocytosis taking place during the stimulation train: early and late endocytosis.
Freshly released synaptic vesicle proteins are preferentially retrieved during the early phase, which is very sensitive to dynasore, an inhibitor of dynamin GTPase activity.
Synaptic vesicle proteins pre‐existing at the plasma membrane before the stimulation are preferentially retrieved during the late phase, which is very sensitive to myristyl trimethyl ammonium bromide (MitMAB), an inhibitor of the dynamin–phospholipid interaction.
AbstractSynaptic endocytosis is essential at nerve terminals to maintain neurotransmitter release by exocytosis. Here, at the neuromuscular junction of synaptopHluorin (spH) transgenic mice, we have used imaging to study exo‐ and endocytosis occurring simultaneously during nerve stimulation. We observed two endocytosis components, which occur sequentially during stimulation. The early component of endocytosis apparently internalizes spH molecules freshly exocytosed. This component was sensitive to dynasore, a blocker of dynamin 1 GTPase activity. In contrast, this early component was resistant to myristyl trimethyl ammonium bromide (MiTMAB), a competitive agent that blocks dynamin binding to phospholipid membranes. The late component of endocytosis is likely to internalize spH molecules that pre‐exist at the plasma membrane before stimulation starts. This component was blocked by MiTMAB, perhaps by impairing the binding of dynamin or other key endocytic proteins to phospholipid membranes. Our study suggests the co‐existence of two sequential synaptic endocytosis steps taking place during stimulation that are susceptible to pharmacological dissection: an initial step, preferentially sensitive to dynasore, that internalizes vesicular components immediately after they are released, and a MiTMAB‐sensitive step that internalizes vesicular components pre‐existing at the plasma membrane surface. In addition, we report that post‐stimulus endocytosis also has several components with different sensitivities to dynasore and MiTMAB.
Complete Genome Sequence of a
Carnation Mottle Virus
Infecting Hop Plants
Authors: Jo Yeonhwa , Choi Hoseong , Cho Won Kyong 
Published: May 2015

ABSTRACT

The
Carnation mottle virus
(CarMV) is a single positive-strand RNA virus belonging to the genus
Carmovirus
. The major natural host for CarMV is the carnation. In this study, using transcriptome data, we provide for the first time a nearly complete genome sequence of CarMV infecting hop plants.
Complete Genome Sequences of
Caldicellulosiruptor
sp. Strain Rt8.B8,
Caldicellulosiruptor
sp. Strain Wai35.B1, and “
Thermoanaerobacter cellulolyticus
”
Authors: Lee Laura L. , Izquierdo Javier A. , Blumer-Schuette Sara E. , Zurawski Jeffrey V. , Conway Jonathan M. , Cottingham Robert W. , Huntemann Marcel , Copeland Alex , Chen I-Min A. , Kyrpides Nikos , Markowitz Victor , Palaniappan Krishnaveni , Ivanova Natalia , Mikhailova Natalia , Ovchinnikova Galina , Andersen Evan , Pati Amrita , Stamatis Dimitrios , Reddy T.B.K. , Shapiro Nicole , Nordberg Henrik P. , Cantor Michael N. , Hua Susan X. , Woyke Tanja , Kelly Robert M. 
Published: May 2015

ABSTRACT

The genus
Caldicellulosiruptor
contains extremely thermophilic, cellulolytic bacteria capable of lignocellulose deconstruction. Currently, complete genome sequences for eleven
Caldicellulosiruptor
species are available. Here, we report genome sequences for three additional
Caldicellulosiruptor
species: Rt8.B8 DSM 8990 (New Zealand), Wai35.B1 DSM 8977 (New Zealand), and “
Thermoanaerobacter cellulolyticus
” strain NA10 DSM 8991 (Japan).
Genome Sequence of Bacillus thuringiensis Strain Btm27, an Egyptian Isolate Highly Toxic to Cotton Leafworm
Authors: Rusconi Brigida , Chen Yue , Koenig Sara S. K. , El-Helow Ehab R. , Eppinger Mark 
Published: May 2015

ABSTRACT

Bacillus thuringiensis
is a potent microbial control agent against insect pests. Here, we present the draft genome of the Egyptian strain Btm27 that shows high toxicity toward the cotton leafworm. The genome contains three insecticidal genes
cry1Ac9
,
cry2Ab1
, and
vip3V
that have been implicated in conferring toxicity toward lepidoptera.
Draft Genome Sequence of Staphylococcus carnosus subsp.
utilis
LTH 7013, Isolated from South Tyrolean Ham
Authors: Müller Anne , Huptas Christopher , Wenning Mareike , Schmidt Herbert , Weiss Agnes 
Published: May 2015

ABSTRACT

Staphylococcus carnosus
is used as a starter culture in meat fermentation, where it contributes to color formation and produces aromatic compounds. Here, we report the first draft genome sequence of an
S. carnosus
subsp.
utilis
strain, LTH 7013, isolated from South Tyrolean ham, with potential application as a starter culture.
Awareness of cervical cancer prevention among mothers of adolescent daughters in Korea: qualitative research
Authors: Kim Hae Won , Kim Duck Hee 
Published: May 2015

ObjectivesKorean adolescent girls are unprepared for cervical cancer prevention due to the lack of a mandatory policy regarding human papilloma virus (HPV) vaccination and school health education regarding cervical cancer. The aim of this study was to determine how aware mothers are about cervical cancer prevention in their adolescent daughters, with a view to developing strategies for expanding primary cervical cancer prevention for adolescent girls through the mothers’ involvement.DesignA qualitative design was employed. Nine mothers with adolescent daughters participated in this study and were interviewed using open-ended questions. The themes were extracted by content analysis.SettingA general living area in Seoul, South Korea.ParticipantsThe snowball method was used to select mothers.ResultsFive themes emerged. In general, the mothers’ awareness of cervical cancer was not clear, and they exhibited a lack of awareness of the importance of having a regular Papanicolaou screening test. The mothers recognised that they were role models for their daughters, and realised and accepted the necessity of educating their daughters regarding cervical cancer; however, they perceived barriers related to the prevention of cervical cancer in their daughters. The mothers recommended enforcing sex education in schools and the provision of financial support for HPV vaccination.ConclusionsThe mothers’ awareness and preparedness with respect to the prevention of cervical cancer in their adolescent daughters were low and inadequate. Mothers should be informed and motivated to play a role in the education of their daughters regarding cervical cancer prevention. Strategies for disseminating information regarding early cervical cancer prevention for adolescent girls are recommended by communicating with both the girls and their mothers and providing them with education regarding cervical cancer prevention.
Platys
Authors: Murukannaiah Pradeep K. , Singh Munindar P. 
Published: May 2015

We introduce a high-level abstraction of location called
place
. A place derives its meaning from a user's physical space, activities, or social context. In this manner, place can facilitate improved user experience compared to the traditional representation of location, which is spatial coordinates. We propose the
Platys
framework as a way to address the special challenges of place-aware application development. The core of Platys is a middleware that (1) learns a model of places specific to each user via
active learning
, a machine learning paradigm that seeks to reduce the user-effort required for training the middleware, and (2) exposes the learned user-specific model of places to applications at run time, insulating application developers from dealing with both low-level sensors and user idiosyncrasies in perceiving places.

We evaluated Platys via two studies. First, we collected place labels and Android phone sensor readings from 10 users. We applied Platys' active learning approach to learn each user's places and found that Platys (1) requires fewer place labels to learn a user's places with a desired accuracy than do two traditional supervised approaches, and (2) learns places with higher accuracy than two unsupervised approaches.
Second, we conducted a developer study to evaluate Platys' efficiency in assisting developers and its effectiveness in enabling usable applications. In this study, 46 developers employed either Platys or the Android location API to develop a place-aware application. Our results indicate that application developers employing Platys, when compared to those employing the Android API, (1) develop a place-aware application faster and perceive reduced difficulty and (2) produce applications that are easier to understand (for developers) and potentially more usable and privacy preserving (for application users).
Design and evaluation of gradual typing for python
Authors: Vitousek Michael M. , Kent Andrew M. , Siek Jeremy G. , Baker Jim 
Published: May 2015

Combining static and dynamic typing within the same language offers clear benefits to programmers. It provides dynamic typing in situations that require rapid prototyping, heterogeneous data structures, and reflection, while supporting static typing when safety, modularity, and efficiency are primary concerns. Siek and Taha (2006) introduced an approach to combining static and dynamic typing in a fine-grained manner through the notion of type consistency in the static semantics and run-time casts in the dynamic semantics. However, many open questions remain regarding the semantics of gradually typed languages.
In this paper we present Reticulated Python, a system for experimenting with gradual-typed dialects of Python. The dialects are syntactically identical to Python 3 but give static and dynamic semantics to the type annotations already present in Python 3. Reticulated Python consists of a typechecker and a source-to-source translator from Reticulated Python to Python 3. Using Reticulated Python, we evaluate a gradual type system and three approaches to the dynamic semantics of mutable objects: the traditional semantics based on Siek and Taha (2007) and Herman et al. (2007) and two new designs. We evaluate these designs in the context of several third-party Python programs.
Chimera
Authors: Park Jason Jong Kyu , Park Yongjun , Mahlke Scott 
Published: May 2015

The demand for multitasking on graphics processing units (GPUs) is constantly increasing as they have become one of the default components on modern computer systems along with traditional processors (CPUs). Preemptive multitasking on CPUs has been primarily supported through context switching. However, the same preemption strategy incurs substantial overhead due to the large context in GPUs. The overhead comes in two dimensions: a preempting kernel suffers from a long preemption latency, and the system throughput is wasted during the switch. Without precise control over the large preemption overhead, multitasking on GPUs has little use for applications with strict latency requirements.
In this paper, we propose Chimera, a collaborative preemption approach that can precisely control the overhead for multitasking on GPUs. Chimera first introduces streaming multiprocessor (SM) flushing, which can instantly preempt an SM by detecting and exploiting idempotent execution. Chimera utilizes flushing collaboratively with two previously proposed preemption techniques for GPUs, namely context switching and draining to minimize throughput overhead while achieving a required preemption latency. Evaluations show that Chimera violates the deadline for only 0.2% of preemption requests when a 15us preemption latency constraint is used. For multi-programmed workloads, Chimera can improve the average normalized turnaround time by 5.5x, and system throughput by 12.2%.
Kinetic Dependence Graphs
Authors: Hassaan Muhammad Amber , Nguyen Donald D. , Pingali Keshav K. 
Published: May 2015

Task graphs or dependence graphs are used in runtime systems to schedule tasks for parallel execution. In problem domains such as dense linear algebra and signal processing, dependence graphs can be generated from a program by static analysis. However, in emerging problem domains such as graph analytics, the set of tasks and dependences between tasks in a program are complex functions of runtime values and cannot be determined statically. In this paper, we introduce a novel approach for exploiting parallelism in such programs. This approach is based on a data structure called the kinetic dependence graph (KDG), which consists of a dependence graph together with update rules that incrementally update the graph to reflect changes in the dependence structure whenever a task is completed.
We have implemented a simple programming model that allows programmers to write these applications at a high level of abstraction, and a runtime within the Galois system [15] that builds the KDG automatically and executes the program in parallel. On a suite of programs that are difficult to parallelize otherwise, we have obtained speedups of up to 33 on 40 cores, out-performing third-party implementations in many cases.
Abstract A28: CBPR and Social Marketing Intersection Model to increase screening mammogram in uninsured and underinsured women: A preliminary evaluation
Authors: Rahman Saleh M.M. , Harris Cynthia M. , Mitchell Miaisha , Soliman Karam F.A. 
Published: May 2015

Abstract
Background: Community Outreach and Preventive Services Core of the FAMU Center of Excellence for Cancer Research, Training and Community service has conceptualized and implemented CBPR and Social Marketing principles to increase screening mammograms among uninsured and underinsured women. This study aims at evaluating the preliminary stage of the five-year project TREE.
Methods: Academic and community partnerships among two local universities, seven community-based organizations, local health departments, regional hospital, local chapters of national organizations and media channels were developed. A preplanned social marketing campaign was implemented to disseminate CBPR methods, and increase awareness about breast cancer prevention. Print materials, information exchange sessions, stakeholder's meeting, community events and media advocacy were used to increase trust and coalition building. The Social marketing campaign has established a slogan ‘A friend is She who Reminds Me’ to exponentially increase participants in screening. Evaluation included process and immediate outcomes in coalition building and cancer prevention. A feedback mechanism was established to identify area of conflict, mechanism of conflict resolution, strengths and weaknesses and to adopt specific strategies to improve.
Results: Fifteen meetings, five community events, 400 information brochures, 300 booklets were distributed in the past seven months and a total of 165 women were recruited for screening mammogram. Pilot testing of five more print materials and two videos were completed.
Conclusion: Preliminary evaluation indicates trustworthy partnership among stakeholders, successful initial recruitment and social marketing campaign. However, strategic improvements needed to meet the demands of community to sustain these partnerships and improve breast cancer prevention efforts.
Citation Format: Saleh M.M. Rahman, Cynthia M. Harris, Miaisha Mitchell, Karam F.A. Soliman. CBPR and Social Marketing Intersection Model to increase screening mammogram in uninsured and underinsured women: A preliminary evaluation. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr A28. doi:10.1158/1538-7755.DISP13-A28
Abstract B02: The Cultural Framework for Health: Transforming the use of culture to reduce cancer health disparities
Authors: Singer Marjorie Kagawa 
Published: May 2015

Abstract
Culture is currently poorly defined and even more poorly operationalized in cancer research. Such practice renders culture irrelevant to the risk factors known or suspected to impact disease prevalence, morbidity, and mortality in diverse population groups. Consequently, it is not surprising that analyses of study data finds culture to contribute negligible explanatory weight to the variance of health outcomes for diverse populations who bear an undue burden of cancer, and the opportunity to identify the pathways of this panhuman process remain invisible. Research studies miss scientifically-grounded concepts and methods that likely account for both population and individual variations in health outcomes. NIH's OBSSR funded a report to change science when researchers consider culture, a fundamental human process, as a factor in health outcomes. This report provides multiple scientifically proven approaches by which to integrate the dynamic and adaptive nature of culture into research. These multiple factors that inform the cultural process require that we reassess how we frame health issues, how we design interventions, how we measure the impact of interventions, and for whom, by whom, and why. Each step will require further theorizing and research, but this report provides a first step by providing a theoretical and practical framework to use to build the foundation needed to pursue this line of inquiry to identify the fundamental role of culture in framing behavior and ways of life that influence mental and physical responses to cancer. Application of this framework could move the science of cancer health disparities research and practice forward in more promising and effective directions.
Citation Format: Marjorie Kagawa Singer. The Cultural Framework for Health: Transforming the use of culture to reduce cancer health disparities. [abstract]. In: Proceedings of the Sixth AACR Conference: The Science of Cancer Health Disparities; Dec 6–9, 2013; Atlanta, GA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2014;23(11 Suppl):Abstract nr B02. doi:10.1158/1538-7755.DISP13-B02