bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell cultivation under different gravitational loads using a novel random positioning incubator
Authors: Benavides Damm Tatiana , Walther Isabelle , Wüest Simon L. , Sekler Jörg , Egli Marcel 
Published: December 2013

ABSTRACTImportant in biotechnology is the establishment of cell culture methods that reflect the in vivo situation accurately. One approach for reaching this goal is through 3D cell cultivation that mimics tissue or organ structures and functions. We present here a newly designed and constructed random positioning incubator (RPI) that enables 3D cell culture in simulated microgravity (0 g). In addition to growing cells in a weightlessness‐like environment, our RPI enables long‐duration cell cultivation under various gravitational loads, ranging from close to 0 g to almost 1 g. This allows the study of the mechanotransductional process of cells involved in the conversion of physical forces to an appropriate biochemical response. Gravity is a type of physical force with profound developmental implications in cellular systems as it modulates the resulting signaling cascades as a consequence of mechanical loading. The experiments presented here were conducted on mouse skeletal myoblasts and human lymphocytes, two types of cells that have been shown in the past to be particularly sensitive to changes in gravity. Our novel RPI will expand the horizon at which mechanobiological experiments are conducted. The scientific data gathered may not only improve the sustainment of human life in space, but also lead to the design of alternative countermeasures against diseases related to impaired mechanosensation and downstream signaling processes on earth. Biotechnol. Bioeng. 2014;111: 1180–1190. © 2013 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.
Changing Behaviour: Successful Environmental Programmes in the Workplace
Authors: Young William , Davis Matthew , McNeill Ilona M. , Malhotra Bindu , Russell Sally , Unsworth Kerrie , Clegg Chris W. 
Published: December 2013

AbstractThere is an increasing focus on improving the pro‐environmental attitudes, behaviour and habits of individuals, whether at home, in education, travelling, shopping or in the workplace. This article focuses on the workplace by conducting a multi‐disciplinary literature review of research that has examined the influence of organization‐based behaviour change initiatives. The review includes only research evidence that measured actual environmental performance (e.g. energy use) rather than solely using self‐reported methods (e.g. questionnaires). The authors develop an ‘employee pro‐environmental behaviour’ (e‐PEB) framework, which contains individual, group, organizational and contextual factors that have predictive relevance across different behaviours and organizations. The review shows that the strongest predictors are environmental awareness, performance feedback, financial incentives, environmental infrastructure, management support and training. A key finding from this review is that attitude change is not necessarily a pre‐requisite for behaviour change in the workplace. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment
The Impact of Business Strategies on Online Sustainability Disclosures
Authors: Amran Azlan , Ooi Say Keat , Mydin Riduan Toani , Devi S. Susela 
Published: December 2013

AbstractWith increasing attention being paid to corporate sustainability, pressure from stakeholders, especially customers, is forcing companies to implement sustainability strategies and practices that express their commitment to sustainable development, and engage with stakeholders through voluntary sustainability disclosure. To better understand the sustainability disclosure mechanisms from a business strategy perspective, this study investigates the influence of business strategy, formulated by customer groups, on online sustainability disclosure. It provides empirical observation of Malaysian public‐listed companies based on the combined lens of stakeholder and legitimacy theory. Despite the comparatively low level of corporate sustainability disclosure in Malaysia, this content analysis of online sustainability information disclosure reveals that the companies with more diversified product lines disclose more sustainability information, and that the corporate sustainability effort is significantly related to brand name. Hence, companies should be encouraged to proactively improve their sustainability performance and disclose more sustainability information in order to strengthen their brand names. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment
Understanding the effects of adolescent girls' social positions within peer groups on exchange practices
Authors: Gentina Elodie 
Published: December 2013

ABSTRACTMost adolescent consumer research focuses on individual variables (e.g. susceptibility to peers' influence), without studying the peer group structure. This research investigates the role of adolescent girls' social position within their networks in exchange practices with friends. By using several different methods (social network method, participant observations and semi‐structured interviews), this study reveals that different forms of clothing exchanges (sharing, lending and borrowing) associated with different social motives (needs for socialising, experiencing, belonging, distinguishing and influencing) evolve differently, depending on adolescent girls' social positions (clique member, liaison and isolate). Whereas clique members engage in sharing practices to socialise and share experiences with their friends, isolated adolescent girls engage in other activities (e.g. asking to borrow clothes) as a way to cope with feelings of loneliness. Some liaisons limit their exchange practices to loans, to emphasise their leadership, but most of them avoid sharing clothes in an attempt to preserve their uniqueness. These results suggest an extended view of materialism, with greater emphasis on use and social motives than on acquisition and its utility. Accordingly, this study proposes a new conceptualisation of materialism, as the extent to which adolescents engage in exchange practices to construct and maintain their social identities. Copyright © 2013 John Wiley & Sons, Ltd.
A star in the eye: cat scratch neuroretinitis
Authors: Gajula Viswanath , Kamepalli Ravi , Kalavakunta Jagadeesh K. 
Published: December 2013

Key Clinical MessageNeuroretinitis is one of the atypical presentations of Cat scratch disease (CSD). Fundoscopy showed star pattern macular exudates. CSD should be considered in patients who present with blurry vision and typical fundoscopic findings.
The influence of complications on the costs of complex cancer surgery
Authors: Short Marah N. , Aloia Thomas A. , Ho Vivian 
Published: December 2013

BACKGROUNDIt is widely known that outcomes after cancer surgery vary widely, depending on interactions between patient, tumor, neoadjuvant therapy, and provider factors. Within this complex milieu, the influence of complications on the cost of surgical oncology care remains unknown. The authors examined rates of Patient Safety Indicator (PSI) occurrence for 6 cancer operations and their association with costs of care.METHODSThe Agency for Healthcare Research and Quality (AHRQ) PSI definitions were used to identify patient safety‐related complications in Medicare claims data. Hospital and inpatient physician claims for the years 2005 through 2009 were analyzed for 6 cancer resections: colectomy, rectal resection, pulmonary lobectomy, pneumonectomy, esophagectomy, and pancreatic resection. Risk‐adjusted regression analyses were used to measure the association between each PSI and hospitalization costs.RESULTSOverall PSI rates ranged from a low of 0.01% for postoperative hip fracture to a high of 2.58% for respiratory failure. Death among inpatients with serious treatable complications, postoperative respiratory failure, postoperative thromboembolism, and accidental puncture/laceration were >1% for all 6 cancer operations. Several PSIs—including decubitus ulcer, death among surgical inpatients with serious treatable complications, and postoperative thromboembolism—raised hospitalization costs by ≥20% for most cancer surgery types. Postoperative respiratory failure resulted in a cost increase >50% for all cancer resections.CONCLUSIONSThe consistently higher costs associated with cancer surgery PSIs indicate that substantial health care savings could be achieved by targeting these indicators for quality improvement. Cancer 2014;120:1035–1041. © 2013 The Authors. Cancer published by Wiley Periodicals, Inc. on behalf of American Cancer Society.
Comparative effectiveness study of patient‐reported outcomes after proton therapy or intensity‐modulated radiotherapy for prostate cancer
Authors: Hoppe Bradford S. , Michalski Jeff M. , Mendenhall Nancy P. , Morris Christopher G. , Henderson Randal H. , Nichols Romaine C. , Mendenhall William M. , Williams Christopher R. , Regan Meredith M. , Chipman Jonathan J. , Crociani Catrina M. , Sandler Howard M. , Sanda Martin G. , Hamstra Daniel A. 
Published: December 2013

BACKGROUNDData continue to emerge on the relative merits of different treatment modalities for prostate cancer. The objective of this study was to compare patient‐reported quality‐of‐life (QOL) outcomes after proton therapy (PT) and intensity‐modulated radiation therapy (IMRT) for prostate cancer.METHODSA comparison was performed of prospectively collected QOL data using the Expanded Prostate Cancer Index Composite (EPIC) questionnaire. QOL data were collected during the first 2 years after treatment for men who received PT and IMRT. PT was delivered to 1243 men at a single center at doses from 76 grays (Gy) to 82 Gy. IMRT was delivered to 204 men who were included in the Prostate Cancer Outcomes and Satisfaction with Treatment Quality Assessment (PROSTQA) study in doses from 75.6 Gy to 79.4 Gy. The Wilcoxon rank‐sum test was used to compare EPIC outcomes by modality using baseline‐adjusted scores at different time points. Individual questions were assessed by converting to binary outcomes and testing with generalized estimating equations.RESULTSNo differences were observed in summary score changes for bowel, urinary incontinence, urinary irritative/obstructive, and sexual domains between the 2 cohorts. However, more men who received IMRT reported moderate/big problems with rectal urgency (P = 0.02) and frequent bowel movements (P = 0.05) than men who received PT.CONCLUSIONSThere were no differences in QOL summary scores between the IMRT and PT cohorts during early follow‐up (up to 2‐years). Response to individual questions suggests possible differences in specific bowel symptoms between the 2 cohorts. These outcomes highlight the need for further comparative studies of PT and IMRT. Cancer 2014;120:1076–1082. © 2013 The Authors. Cancer published by Wiley Periodicals, Inc. on behalf of American Cancer Society.
National trends in pancreatic cancer outcomes and pattern of care among Medicare beneficiaries, 2000 through 2010
Authors: Wang Yun , Schrag Deborah , Brooks Gabriel A. , Dominici Francesca 
Published: December 2013

BACKGROUNDPancreatic cancer is a major cause of morbidity and mortality in the Medicare population. Whether the health care burden of pancreatic cancer has changed over the last decade is unknown.METHODSThe authors used Medicare data from 2000 to 2010 to identify beneficiaries aged ≥ 65 years who were hospitalized for the management of pancreatic cancer. Annual trends were estimated for the age‐sex‐race–adjusted initial hospitalization rate, the age‐sex‐race‐comorbidity–adjusted 1‐year mortality rate after initial hospitalization, age‐sex‐race‐comorbidity–adjusted procedure rates, 1‐year all‐cause rehospitalizations after initial pancreatic cancer hospitalization, and mean inflation‐adjusted Medicare payment for initial hospitalization.RESULTSA total of 130,728 patients had ≥ 1 hospitalizations for pancreatic cancer and were identified from 56,642,071 beneficiaries during the study period. The age‐sex‐race–adjusted rate of initial hospitalization for pancreatic cancer was 50 per 100,000 person‐years in 2010, representing a 0.5% annual increase since 2000 (95% confidence interval [95% CI], 0.3%‐0.7%). In the same period, the age‐sex‐race‐comorbidity–adjusted 1‐year mortality rate decreased by 4.4% (95% CI, 3.9%‐4.9%), and the age‐sex‐race‐comorbidity–adjusted surgical resection rate increased by 6.9% (95% CI, 6.4%‐7.5%). The mean inflation‐adjusted Medicare payment for the initial hospitalization decreased, from $14,118 in 2000 to $13,318 in 2010, and the number of 1‐year all‐cause rehospitalizations after the initial hospitalization increased from 0.75 per patient in 2000 to 0.82 per patient in 2009 (all P < .001).CONCLUSIONSFor Medicare fee‐for‐service beneficiaries, initial pancreatic cancer hospitalization, surgical resection, and rehospitalization rates increased, but 1‐year mortality rates declined over the last decade. Cancer 2014;120:1050–1058. © 2013 American Cancer Society.
Coexpression of three opsins in cone photoreceptors of the salamander Ambystoma tigrinum
Authors: Isayama Tomoki , Chen Ying , Kono Masahiro , Fabre Eduard , Slavsky Michael , DeGrip Willem J. , Ma Jian‐Xing , Crouch Rosalie K. , Makino Clint L. 
Published: December 2013

ABSTRACTAlthough more than one type of visual opsin is present in the retina of most vertebrates, it was thought that each type of photoreceptor expresses only one opsin. However, evidence has accumulated that some photoreceptors contain more than one opsin, in many cases as a result of a developmental transition from the expression of one opsin to another. The salamander UV‐sensitive (UV) cone is particularly notable because it contains three opsins (Makino and Dodd [1996] J Gen Physiol 108:27–34). Two opsin types are expressed at levels more than 100 times lower than the level of the primary opsin. Here, immunohistochemical experiments identified the primary component as a UV cone opsin and the two minor components as the short wavelength‐sensitive (S) and long wavelength‐sensitive (L) cone opsins. Based on single‐cell recordings of 156 photoreceptors, the presence of three components in UV cones of hatchlings and terrestrial adults ruled out a developmental transition. There was no evidence for multiple opsin types within rods or S cones, but immunohistochemistry and partial bleaching in conjunction with single‐cell recording revealed that both single and double L cones contained low levels of short wavelength‐sensitive pigments in addition to the main L visual pigment. These results raise the possibility that coexpression of multiple opsins in other vertebrates was overlooked because a minor component absorbing at short wavelengths was masked by the main visual pigment or because the expression level of a component absorbing at long wavelengths was exceedingly low. J. Comp. Neurol. 522:2249–2265, 2014. © 2013 Wiley Periodicals, Inc.
Differential distribution and energy status–dependent regulation of the four CART neuropeptide genes in the zebrafish brain
Authors: Akash G. , Kaniganti Tarun , Tiwari Neeraj Kumar , Subhedar Nishikant K. , Ghose Aurnab 
Published: December 2013

ABSTRACTThe cocaine‐ and amphetamine‐regulated transcript (CART) neuropeptide has been implicated in the neural regulation of energy homeostasis across vertebrate phyla. By using gene‐specific in situ hybridization, we have mapped the distribution of the four CART mRNAs in the central nervous system of the adult zebrafish. The widespread neuronal expression pattern for CART 2 and 4 suggests a prominent role for the peptide in processing sensory information from diverse modalities including olfactory and visual inputs. In contrast, CART 1 and 3 have a much more restricted distribution, predominantly located in the nucleus of the medial longitudinal fasciculus (NMLF) and entopeduncular nucleus (EN), respectively. Enrichment of CART 2 and 4 in the preoptic and tuberal areas emphasizes the importance of CART in neuroendocrine functions. Starvation resulted in a significant decrease in CART‐positive cells in the nucleus recessus lateralis (NRL) and nucleus lateralis tuberis (NLT) hypothalamic regions, suggesting a function in energy homeostasis for these neurons. Similarly, the EN emerges as a novel energy status–responsive region. Not only is there abundant and overlapping expression of CART 2, 3, and 4 in the EN, but also starvation induced a decrease in CART‐expressing neurons in this region. The cellular resolution mapping of CART mRNA and the response of CART‐expressing nuclei to starvation underscores the importance of CART neuropeptide in energy processing. Additionally, the regional and gene‐specific responses to energy levels suggest a complex, interactive network whereby the four CART gene products may have nonredundant functions in energy homeostasis. J. Comp. Neurol. 522:2266–2285, 2014. © 2013 Wiley Periodicals, Inc.
Lateral habenula and the rostromedial tegmental nucleus innervate neurochemically distinct subdivisions of the dorsal raphe nucleus in the rat
Authors: Sego Chemutai , Gonçalves Luciano , Lima Leandro , Furigo Isadora C. , Donato Jose , Metzger Martin 
Published: December 2013

ABSTRACTThe lateral habenula (LHb) is an epithalamic structure differentiated in a medial (LHbM) and a lateral division (LHbL). Together with the rostromedial tegmental nucleus (RMTg), the LHb has been implicated in the processing of aversive stimuli and inhibitory control of monoamine nuclei. The inhibitory LHb influence on midbrain dopamine neurons has been shown to be mainly mediated by the RMTg, a mostly GABAergic nucleus that receives a dominant input from the LHbL. Interestingly, the RMTg also projects to the dorsal raphe nucleus (DR), which also receives direct LHb projections. To compare the organization and transmitter phenotype of LHb projections to the DR, direct and indirect via the RMTg, we first placed injections of the anterograde tracer Phaseolus vulgaris leucoagglutinin into the LHb or the RMTg. We then confirmed our findings by retrograde tracing and investigated a possible GABAergic phenotype of DR‐projecting RMTg neurons by combining retrograde tracing with in situ hybridization for GAD67. We found only moderate direct LHb projections to the DR, which mainly emerged from the LHbM and were predominantly directed to the serotonin‐rich caudal DR. In contrast, RMTg projections to the DR were more robust, emerged from RMTg neurons enriched in GAD67 mRNA, and were focally directed to a distinctive DR subdivision immunohistochemically characterized as poor in serotonin and enriched in presumptive glutamatergic neurons. Thus, besides its well‐acknowledged role as a GABAergic control center for the ventral tegmental area (VTA)–nigra complex, our findings indicate that the RMTg is also a major GABAergic relay between the LHb and the DR. J. Comp. Neurol. 522:1454–1484, 2014. © 2013 Wiley Periodicals, Inc.
Evolutionary Pathways of Environmental Management in UK Companies
Authors: Ormazabal M. , Sarriegi J. M. , Barkemeyer R. , Viles E. , McAnulla F. 
Published: December 2013

ABSTRACTThis research focuses on the evolution of environmental management within firms. It is based on a survey and semi‐structured interviews conducted among UK companies. Building on an evolutionary conceptual model, consisting of several maturity stages, we identify ‘milestones’, i.e. common practices companies engage in when progressing through specific maturity stages. Whilst clear commonalities can be identified, most notably linked to the order of maturity stages they progressed through and the emergence of commonly acknowledged practices linked to the individual maturity stages, the results also reveal a number of within‐sample patterns regarding the companies’ progression through the different maturity stages. It can be concluded that the maturity stages and practices identified in this research can provide valuable guidance for firms aiming to make progress in environmental matters, as they help them to identify in which maturity stage they are and sets out steps that they can take to move forward. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment.
The Significance of the Non‐Timber Forest Products Policy for Forest Ecology Management: A Case Study in West Bengal, India
Authors: Ghosal Somnath 
Published: December 2013

ABSTRACTA precise non‐timber forest products (NTFPs) policy can play an important role in the preservation of the forest resource base. By promoting systematic and sustainable harvesting of forest products, the exploitation of forest resources can be reduced and the socio‐economic status of marginal forest dwellers can also be improved, which will subsequently reduce illegal timber harvesting. In India, brief guidelines for the harvesting of NTFPs are placed in the Joint Forest Management resolution. However, these guidelines are not equally effective in all states because of micro‐level geophysical variances, forest–human relationships and the characteristics of the forests. Therefore, some south Indian states such as Tamil Nadu, Orissa and Andhra Pradesh have developed their own NTFP harvesting policy for the sustainable management of forests through the socio‐economic enhancement of forest dwellers. However, in West Bengal the central government's guidelines are still followed. This paper examines the need for a precise NTFP harvesting policy for the management of forests in West Bengal through the socio‐economic improvement of forest fringe tribal communities. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment
Environmental Commitment and Economic Performance – Short‐Term Pain for Long‐Term Gain
Authors: Teng Mei‐Jane , Wu Shih‐Ying , Chou Stephen Jui‐Hsien 
Published: December 2013

ABSTRACTAlthough an increasing number of studies have begun to investigate the relationship between firms' environmental practices and economic performance, one of the issues that have been neglected involves the time lag between environmental investment and economic performance, as well as the tradeoff between short‐term costs and long‐term benefits. By utilizing a sample of publicly listed corporations in Taiwan over the period 1996–2008, we find that the relationship between economic performance and ISO certification is neither strictly negative nor strictly positive, but is instead U shaped. The evidence suggests that, although a firm bears costs for environmental management in the short term, the benefits of environmental management accumulate over time after environmental certification, and a firm benefits from environmental management in the long term. The result also provides an explanation for the mixed evidence on the relationship between proactive environmental strategies and economic performance by documenting a non‐monotonic relationship between environmental management and firm performance. The finding thus implies that government subsidies for ISO certification can be rationalized, since they help firms to overcome the barrier associated with the costs of certification, and encourage them to obtain ISO certification. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment