bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogeography of apomictic and outcrossing individuals in invasive and native populations of Ardisia crenata (Primulaceae)
Authors: Noyori Wataru , Isagi Yuji , Nakamura Naoto , Tagane Shuichiro , Celis Gerardo , Kitajima Kaoru 
Published: July 2024

AbstractPhylogeography of invasive exotic plant species is crucial for identifying the source population from which a given invading population has originated. Ardisia crenata Sims, a shade tolerant shrub native to East Asia, is one of the most serious invasive exotic plants in Florida, North America. The objective of this study is to investigate the genetic differentiation and phylogeographical relationships among A. crenata native populations in Japan and invasive populations in Florida. We analyzed DNA from 188 individuals gathered from eight populations in Japan and four subpopulations in Florida using double‐digestion restriction‐associated DNA sequencing (ddRAD‐seq) and estimated a maximum likelihood phylogenetic tree among them. The results revealed four genetic clusters, with a striking dominance of genetically identical clonal individuals within the invading populations in Florida and four Honshu populations in Japan. These groups were genetically close to each other and to one cultivated individual sampled in Japan. In contrast, the individuals from Miyazaki, Yakushima, Kochi, and Iriomote in southwestern Japan were genetically distinct from Honshu and Florida individuals, showing high genetic diversity indicative of outcrossing. Given that A. crenata does not exhibit a vegetative clonal habit and all wild and cultivated plants regenerate from seeds, these results suggest that Honshu and Florida populations exclusively reproduced with seeds produced by apomixis.
Let's talk populist? A survey experiment on effects of (non‐) populist discourse on vote choice
Authors: KITTEL REBECCA C. 
Published: July 2024

AbstractPopulism research has found much scholarly and public attention alike in recent years. Most research has focused on how populism can be defined, assessed or even measured. Even though there are emerging studies on populist messages, few of them have paid attention on causally identifying ways in which discourse can affect support for populist actors. This article positions itself within this gap and aims to answer which discursive elements make (non‐)populist messages appealing to varying groups of people. To answer this research question, I conducted a novel survey experiment on vote choice in Germany from December 2020 to January 2021 with N = 3325. Respondents were asked to choose between two candidate statements that displayed varying discursive elements. Thus, the experiment causally tested whether people‐centric rhetoric, blame attributive languages or populist style focusing on language complexity drive the populist vote. Results show that a neutral form of blame attribution, namely towards politicians, had the highest probability of driving vote choice, irrespective of respondents' underlying ideological preferences or populist attitudes. Simple language nearly always has a negative effect on vote choice, whereas people‐centrism adds a positive touch. These results show that there may be an increasing dissatisfaction with democracy that is voiced by blaming political elites for the malfunctioning of society.
DNA fork remodeling proteins, Zranb3 and Smarcal1, are uniquely essential for aging hematopoiesis
Authors: Kushinsky Saul , Puccetti Matthew V. , Adams Clare M. , Shkundina Irina , James Nikkole , Mahon Brittany M. , Michener Peter , Eischen Christine M. 
Published: July 2024

AbstractOver a lifetime, hematopoietic stem and progenitor cells (HSPCs) are forced to repeatedly proliferate to maintain hematopoiesis, increasing their susceptibility to DNA damaging replication stress. However, the proteins that mitigate this stress, protect HSPC replication, and prevent aging‐driven dysregulation are unknown. We report two evolutionarily conserved, ubiquitously expressed chromatin remodeling enzymes with similar DNA replication fork reversal biochemical functions, Zranb3 and Smarcal1, have surprisingly specialized roles in distinct HSPC populations. While both proteins actively mitigate replication stress and prevent DNA damage and breaks during lifelong hematopoiesis, the loss of either resulted in distinct biochemical and biological consequences. Notably, defective long‐term HSC function, revealed with bone marrow transplantation, caused hematopoiesis abnormalities in young mice lacking Zranb3. Aging significantly worsened these hematopoiesis defects in Zranb3‐deficient mice, including accelerating the onset of myeloid‐biased hematopoietic dysregulation to early in life. Such Zranb3‐deficient HSPC abnormalities with age were driven by accumulated DNA damage and replication stress. Conversely, Smarcal1 loss primarily negatively affected progenitor cell functions that were exacerbated with aging, resulting in a lymphoid bias. Simultaneous loss of both Zranb3 and Smarcal1 compounded HSPC defects. Additionally, HSPC DNA replication fork dynamics had unanticipated HSPC type and age plasticity that depended on the stress and Zranb3 and/or Smarcal1. Our data reveal both Zranb3 and Smarcal1 have essential HSPC cell intrinsic functions in lifelong hematopoiesis that protect HSPCs from replication stress and DNA damage in unexpected, unique ways.
Development and validation of a high‐quality simulator with exchangeable peritoneum for transabdominal preperitoneal laparoscopic inguinal hernia repair
Authors: Shibuya Ayako , Isobe Yoh , Nishihara Yuichi , Matsumoto Sumio , Nagayasu Takeshi , Matsumoto Keitaro 
Published: July 2024

AbstractIntroductionPractical simulation training with proper haptic feedback and the fragility of the human body is required to overcome the long learning curve associated with laparoscopic inguinal hernia repair (LIHR). However, few hernia models accurately reflect the texture and fragility of the human body. Therefore, in this study, we developed a novel model for transabdominal preperitoneal (TAPP) LIHR training and evaluated its validity.MethodsWe developed a high‐quality mock peritoneum with a hydrated polyvinyl alcohol layer and a unique two‐way crossing cellulose fiber layer. To complete the simulation, the peritoneum was adhered to a urethane foam inguinal base with surgical landmarks. Participants could perform all the procedures required for the TAPP LIHR. Twenty‐four surgeons performed TAPP LIHR simulation using a novel simulator. Their opinions were rated on a 5‐point Likert scale. Additionally, 6 surgical residents and 10 surgical experts performed the procedure. Their performance was evaluated using the TAPP checklist score and procedure time.ResultsMost participants strongly agreed that the TAPP LIHR simulator with an exchangeable peritoneum model was useful. The participants agreed on the model fidelity for tactile sensation, forceps handling, and humanlike anatomy. In comparisons between surgical residents and experts, the experts had significantly higher scores (10.6 vs. 17.2, p < 0.05) and shorter procedure times (92.3 vs. 55.9 min; p < .05) than did surgical residents.ConclusionsWe developed a high‐quality exchangeable peritoneal model that mimics the human peritoneum's texture and fragility. This model enhances laparoscopic simulation training, potentially shortening TAPP LIHR learning curves.
Emergency myelopoiesis in solid cancers
Authors: Aliazis Konstantinos , Yenyuwadee Sasitorn , Phikulsod Ployploen , Boussiotis Vassiliki A. 
Published: July 2024

SummaryCells of the innate and adaptive immune systems are the progeny of haematopoietic stem and progenitor cells (HSPCs). During steady‐state myelopoiesis, HSPC undergo differentiation and proliferation but are called to respond directly and acutely to various signals that lead to emergency myelopoiesis, including bone marrow ablation, infections, and sterile inflammation. There is extensive evidence that many solid tumours have the potential to secrete classical myelopoiesis‐promoting growth factors and other products able to mimic emergency haematopoiesis, and to aberrantly re‐direct myeloid cell development into immunosuppressive cells with tumour promoting properties. Here, we summarize the current literature regarding the effects of solid cancers on HSPCs function and discuss how these effects might shape antitumour responses via a mechanism initiated at a site distal from the tumour microenvironment.
Pseudoephedrine for ejaculatory dysfunction after retroperitoneal lymph node dissection in testicular cancer
Authors: Conduit Ciara , Lewin Jeremy , Hong Wei , Sim Ie‐Wen , Ahmad Gulfam , Leonard Matt , O'Haire Sophie , Moody Mary , Hutchinson Amanda D. , Lawrentschuk Nathan , Thomas Benjamin , Dhillon Haryana M. , Tran Ben 
Published: July 2024

ObjectiveTo assess the impact of ejaculatory dysfunction (EjD; failure of emission or retrograde ejaculation) on health‐related quality of life (HRQoL) after retroperitoneal lymph node dissection (RPLND) for testicular cancer and explore the efficacy of pseudoephedrine hydrochloride as treatment.Patients and MethodsIn a single arm, phase II trial, patients at ≥6 months after RPLND were invited to complete patient‐reported outcome measures (European Organisation for Research and Treatment of Cancer [EORTC] quality of life questionnaire [QLQ]‐30‐item core, EORTC QLQ‐testicular cancer‐26, and Brief Male Sexual Function Inventory) evaluating HRQoL and sexual function in follow‐up (ACTRN12622000537752/12622000542796). If EjD was reported, post‐ejaculatory urine ± semen analysis was undertaken. In eligible patients, pseudoephedrine hydrochloride 60 mg was administered orally every 6 h for six doses. The primary endpoint was sperm count >39 million sperm/ejaculate (>5th centile) following treatment. The trial was powered to detect a clinically relevant 36% achieving sperm count of >39 million sperm/ejaculate. Secondary endpoints included semen volume >1.5 mL, total motile sperm count, safety, and HRQoL impacts.ResultsOf the 58 patients enrolled, the median (interquartile range [IQR]) age was 35 (29–41) years, with a median (IQR) of 37 (18–60) months from RPLND. EjD was reported in 33 (57%), including 27/52 (52%) receiving follow‐up at our centre. There were no differences in global HRQoL; however, role functioning (P = 0.045), sexual problems (P < 0.005), and sexual enjoyment (P = 0.005) was poorer if EjD was present. In all, 24/33 (73%) patients with EjD consented to pseudoephedrine treatment. Of 22 evaluable patients, four (18%) achieved a sperm count of >39 million/ejaculate (P = 0.20), and four (18%) had a semen volume of >1.5 mL (P = 0.20). There was a mean increase of 105 million sperm/ejaculate (P = 0.051) and 1.47 mL increase in semen volume (P = 0.01). No safety concerns arose.ConclusionEjaculatory dysfunction is common after RPLND but did not impact global HRQoL in our cohort. Pseudoephedrine improved EjD for some; however, its efficacy was lower than expected. Pseudoephedrine may be considered on an individualised basis.
Foxq1 activates CB2R with oleamide to alleviate POCD
Authors: Wu Xiaoying , Wu Yuming , Tang Fudong , Wang Yangyang , Li Chenxi , Wu Su , Wang Guangzhi , Zhang Jiaqiang 
Published: July 2024

AbstractPostoperative cognitive dysfunction (POCD) is a major concern, particularly among older adults. This study used social isolation (ISO) and multiomics analyses in aged mice to investigate potential mechanisms underlying POCD development. Aged mice were divided into two groups: ISO and paired housing (PH). Oleamide and the cannabinoid receptor type 2 (CB2R) antagonist AM630 were administered intraperitoneally, while Foxq1 adeno‐associated viral (AAV) vector was injected directly into the hippocampus. Intramedullary tibial surgeries were subsequently performed to establish the POCD models. Behavioral tests comprising the Y‐maze, open field test, and novel object recognition were conducted 2 days after surgery. Hippocampal and serum inflammatory cytokines were assessed. Following surgery, ISO mice demonstrated intensified cognitive impairments and escalated inflammatory markers. Integrative transcriptomic and metabolomic analysis revealed elevated oleamide concentrations in the hippocampus and serum of PH mice, with associative investigations indicating a close relationship between the Foxq1 gene and oleamide levels. While oleamide administration and Foxq1 gene overexpression substantially ameliorated postoperative cognitive performance and systemic inflammation in mice, CB2R antagonist AM630 impeded these enhancements. The Foxq1 gene and oleamide may be crucial in alleviating POCD. While potentially acting through CB2R‐mediated pathways, these factors may modulate neuroinflammation and attenuate proinflammatory cytokine levels within the hippocampus, substantially improving cognitive performance postsurgery. This study lays the groundwork for future research into therapeutic approaches targeting the Foxq1‐oleamide‐CB2R axis, with the ultimate goal of preventing or mitigating POCD.
New insights into the structure of the Yinggehai Basin and its tectonic implications, South China Sea: Evidence from scaled physical models
Authors: Yang Gengxiong , Yin Hongwei , Jia Dong , Wang Hongbin , Wang Wei , Xu Wenqiao 
Published: July 2024

AbstractThe Yinggehai Basin is situated at the junction of Indochina and the northern South China Sea (SCS). The origin of the Yinggehai Basin is generally believed to be controlled by the rotation of the Indochina block along the Red River shear zone (RRSZ), which was formed by the collision of India with Asia during the Oligocene. However, the Eocene structural mechanisms of this basin remain debatable. Some studies suggest that the Eocene reactivation of the palaeo‐suture zone (which serves as a precursor to the RRSZ) has influenced the region. In contrast, others propose that the NNW–SSE extension of the northern SCS caused by the subduction of the palaeo‐SCS towards Borneo in the Eocene has played a significant role. To address these controversies, our study takes into account these two crucial factors using physical analogue modelling. The experimental results, including slow sinistral strike‐slip along the palaeo‐suture zone and the adjacent NNW–SSE extension, successfully explain the observed fault pattern during the Eocene period. It is noteworthy that the former primarily controlled the Eocene structure in the northern region of the Yinggehai Basin, whereas the latter played a pivotal role in shaping the ENE–WSW Eocene structures on the eastern slope of the basin. The westward propagating faults of the Qiongdongnan basin are cut off by the Yinggehai Basin structures at later large‐scale rotation stage. The experiment indicates that the basin evolution exhibits diachronous characteristics, with subsidence in the south occurring later than in the north. Our modelling results provide valuable insights into the key controlling factors that shaped the evolution of the basin during each stage. Furthermore, our findings offer evidence of the interaction between two significant tectonic processes: Indochina extrusion and the opening of the SCS.
Premeiotic deletion of Eif2s2 causes oocyte arrest at the early diplotene stage and apoptosis in mice
Authors: Zhou Wenjun , Li Biao , Wang Zhijuan , Liu Shuang , Wang Weiyong , He Sihui , Chen Ye , Zhang Xiaodan , Zhang Meijia 
Published: July 2024

AbstractEukaryotic translation initiation factor 2 subunit 2 (EIF2S2), a subunit of the heterotrimeric G protein EIF2, is involved in the initiation of translation. Our findings demonstrate that the depletion of Eif2s2 in premeiotic germ cells causes oocyte arrest at the pachytene and early diplotene stages at 1 day postpartum (dpp) and 5 dpp, respectively, and eventually leads to oocyte apoptosis and failure of primordial follicle formation. Further studies reveal that Eif2s2 deletion downregulates homologous recombination‐related and mitochondrial fission‐related protein levels, and upregulates the integrated stress response‐related proteins and mRNA levels. Consistently, Eif2s2 deletion significantly decreases the expression of dictyate genes and compromises mitochondrial function, characterized by elongated shapes, decreased ATP levels and mtDNA copy number, along with an excessive accumulation of reactive oxygen species (ROS) and mitochondrial superoxide. Furthermore, DNA damage response and proapoptotic protein levels increase, while anti‐apoptotic protein levels decrease in Eif2s2‐deleted mice. An increase in oocytes with positive cleaved‐Caspase‐3 and TUNEL signals, alongside reduced Lamin B1 intensity, further indicates oocyte apoptosis. Collectively, Eif2s2 deletion in premeiotic germ cells causes oocyte meiotic arrest at the early diplotene stage by impairing homologous recombination, and eventually leads to oocyte apoptosis mainly through the downregulation of mitochondrial fission‐related proteins, ROS accumulation and subsequent DNA damage.
Nuclear receptor Rev‐erbα alleviates intervertebral disc degeneration by recruiting NCoR–HDAC3 co‐repressor and inhibiting NLRP3 inflammasome
Authors: Zhou Qingshuang , Pu Xiaojiang , Qian Zhuang , Chen Haojie , Wang Nannan , Wang Sinian , Feng Zhenhua , Zhu Zezhang , Wang Bin , Qiu Yong , Sun Xu 
Published: July 2024

AbstractIntervertebral discs (IVDs) are rhythmic tissues that experience daily low‐load recovery. Notably, aging and abnormal mechanical stress predispose IVDs to degeneration due to dysrhythmia‐induced disordered metabolism. Meanwhile, Rev‐erbα acts as a transcriptional repressor in maintaining biorhythms and homeostasis; however, its function in IVD homeostasis and degeneration remains unclear. This study assessed the relationship between low Rev‐erbα expression levels and IVD degeneration. Rev‐erbα deficiency accelerated needle puncture or aging‐induced IVD degeneration, characterized by increased extracellular matrix (ECM) catabolism and nucleus pulposus (NP) cell apoptosis. Mechanistically, Rev‐erbα knockdown in NP cells aggravated rhIL1β‐induced NOD‐like receptor family pyrin domain containing 3 (NLRP3) inflammasome activation, exacerbating the imbalanced ECM and NP cell apoptosis. Meanwhile, blocking NLRP3 inflammasome activation mitigated Rev‐erbα deficiency and needle puncture‐induced IVD degeneration. Particularly, Rev‐erbα mediated the transcriptional repression of the NLRP3 inflammasome via the ligand heme‐binding of nuclear receptor co‐repressor (NCoR) and histone deacetylase 3 (HDAC3) complex. Thus, the increased expression of Rev‐erbα in NP cells following short‐term rhIL1β treatment failed to inhibit NLRP3 transcription in vitro owing to heme depletion. Pharmacological activation of Rev‐erbα in vivo and in vitro alleviated IVD degeneration by altering the NLRP3 inflammasome. Taken together, targeting Rev‐erbα may be a potential therapeutic strategy for alleviating IVD degeneration and its related diseases.
A Digital Approach to Improve Infection Screening Among Solid Organ Transplant Candidates
Authors: Righi Elda , Visentin Alessandro , Mirandola Massimo , Rigo Costanza , Cutone Carmine , Rocchi Matilde , Bonato Lucia , Armellini Maddalena , Caletti Chiara , Onorati Francesco , Biagio Livio San , Luciani Giovanni Battista , Mazzeo Gina , Merighi Mara , Vantini Gianluca , Borin Alex , Boschiero Luigino , Carraro Amedeo , Tacconelli Evelina 
Published: July 2024

ABSTRACTBackgroundPretransplant infection screening (IS) of potential organ recipients is essential to optimal outcome of solid organ transplantation (SOT).MethodsA pre‐post study was performed during 2020–2023 to investigate the impact of the STREAM (Solid organ TRansplant stEwArdship and Multidisciplinary approach) intervention to improve IS in SOT. The intervention, performed in 2022, included the implementation of IS through educational meetings, local guidelines, and the availability of a digital screening tool. The objective of the study was the assessment of IS completion, including a list of 17 laboratory tests and the investigation of vaccination status. The reduction of unnecessary tests was also analyzed. The test of proportions and a multilevel multivariate Poisson regression model were used to compare IS completion before and after STREAM. infectious diseases (ID) consultation and urgent evaluation were investigated as predictors of IS completion.ResultsA total of 171 patients were enrolled, including liver (44%), heart (32%), and kidney (24%) transplant candidates. Mean age was 56 ± 11 years, and most patients (77%) were males. Ninety‐five (56%) patients were included before the intervention and 76 (44%) after STREAM. IS completion increased after STREAM (IRR 1.41, p < 0.001) with significant improvement recorded for seven (39%) IS items. Unnecessary tests decreased by 43% after the intervention. ID consultation (IRR 1.13, p = 0.02) and urgent evaluation (IRR 0.68, p 〈0.001) were predictors of IS improvement.ConclusionsSTREAM was successful in improving IS completion. Further research is needed to investigate the impact of this intervention on posttransplant infections.
Insulin icodec: A novel once‐weekly treatment for diabetes
Authors: Schaffner Hannah , Wiener Jordyn , DeLuca Amanda , Genovese Ariana , Deeb Alexander , Deeb Wasim , Sheikh‐Ali Mae , Sutton David , Gore Ashwini , Berner Jason , Huston Jessica , Goldfaden Rebecca 
Published: July 2024

AbstractAimsTo summarize the results of clinical studies of insulin icodec, an investigational insulin analog designed for once‐weekly administration, in adults with type 1 and type 2 diabetes.MethodsThirteen published articles describing clinical studies of insulin icodec were identified in PubMed, and data pertinent to key study outcomes were selected for inclusion in this review.ResultsIn insulin‐naïve and insulin‐treated individuals, icodec demonstrated efficacy in glycaemic control superior or noninferior to that of insulins glargine U100, glargine U300 and degludec. Icodec exhibited a safety profile comparable to marketed insulins, with the exception of hypoglycaemic event rates.ConclusionsAs a once‐weekly alternative to daily basal insulin, icodec is expected to improve patient adherence and satisfaction, reducing the required number of injections per year from 365 to 52 and providing a dosing option potentially attractive to a wide range of insulin users. However, clinical data suggest a notable risk of hypoglycaemia with weekly icodec administration, especially in individuals with type 1 diabetes.
Medication adherence to sodium‐glucose cotransporter‐2 inhibitors versus glucagon‐like peptide‐1 receptor agonists: A meta‐analysis
Authors: Johnson Conner E. , Sussman Whitney B. , Weeda Erin R. 
Published: July 2024

AbstractAimTo perform a meta‐analysis comparing real‐world medication adherence to sodium–glucose cotransporter‐2 inhibitors (SGLT2is) versus glucagon‐like peptide‐1 receptor agonists (GLP‐1RAs).Materials and MethodsA systematic search of Medline and Embase was conducted through October 2023. To meet inclusion criteria, articles had to be published in full text form and directly compare medication adherence to SGLT2is versus GLP‐1RAs in adults. Only studies evaluating real‐world data and utilizing the proportion of days covered (PDC) to measure adherence were included. Non‐adherence, defined as the proportion of patients with a PDC <80%, was the primary outcome. A subgroup analysis evaluating results among studies conducted in the United States was performed.ResultsWe identified eight studies evaluating 205 103 patients for inclusion. The most common country from which the data was derived was the United States (n = 5 studies). Upon meta‐analysis, we observed no difference in non‐adherence (i.e. PDC <80%) to SGLT2is versus GLP‐1RAs (relative risk = 0.86; 95% confidence interval = 0.72–1.02). In the analysis, including only US studies, SGLT2i use was associated with a 23% lower risk of non‐adherence compared with GLP‐1RA use (relative risk = 0.77; 95% confidence interval = 0.72–0.82).ConclusionsIn this meta‐analysis of eight studies that included approximately 200 000 patients, there was no difference in adherence to SGLT2is versus GLP‐1RAs. However, SGLT2i use was associated with higher adherence when the analysis was limited to US studies.