bluuebunny/crossref_metadata_2025_split huggingface dataset:
TRAIL and TRAIL receptors in patients with laryngeal cancer
Authors: Erkul Evren , Kucukodaci Zafer , Pinar Dogan , Gungor Atila , Alparslan Babayigit Mustafa , Kurt Onuralp , Cincik Hakan 
Published: March 2015

AbstractBackgroundTumor necrosis factor–related associated‐inducing ligand (TRAIL) is a death ligand currently under clinical trials for laryngeal carcinoma.MethodsParaffin‐embedded tissues from 40 patients with laryngeal carcinoma and 20 patients with benign laryngeal pathologies were retrospectively analyzed using immunohistochemistry in terms of distribution and intensity, and for final analysis of immunoreactivity of receptors, H‐score was used. The study group was assessed in terms of localization, lymph node staging, tumor stage, overall survival, disease‐free survival, locoregional control, perineural invasion, and vascular invasion.ResultsThe H‐score of decoy‐R2 (DcR2) staining were increased significantly in tumor tissue (p = .04). A significantly greater increase in terms of H‐score of DR5 receptor staining (p = .06) was detected in tumor tissue.ConclusionTRAIL‐mediated gene therapy may not be effective. Indeed, the findings may indicate treatment resistance. TRAIL and TRAIL receptor levels were not associated with prognosis © 2015 Wiley Periodicals, Inc. Head Neck 38: E535–E541, 2016
Toxicity and efficacy of accelerated radiotherapy with concurrent weekly cisplatin for locally advanced head and neck carcinoma
Authors: Driessen Chantal M. L. , Janssens Geert O. , van der Graaf Winette T. A. , Takes Robert P. , Merkx Thijs A. W. , Melchers Willem J. G. , Kaanders Hans A. M. , van Herpen Carla M. L. 
Published: March 2015

AbstractBackgroundThe purpose of this report was to present the results of accelerated radiotherapy (RT) with concomitant weekly cisplatin in head and neck cancer.MethodsOne hundred six patients received concomitant cisplatin 40 mg/m2 weekly with accelerated RT up to a dose of 68 Gy over 5.5 weeks.ResultsNinety‐nine percent of the patients received planned RT and 90% received ≥5 cycles of cisplatin. Moist desquamation of skin developed in 45% and confluent mucositis in 82%. Feeding tubes were required in 79% of the patients, and after 12 months in 4%. One patient developed nephrotoxicity. Three‐year locoregional control, disease‐free survival (DFS), and overall survival (OS) were 72%, 54%, and 61%, respectively. Human papillomavirus (HPV) status was positive on polymerase chain reaction (PCR) and p16 in 11 of 50 patients with oropharyngeal carcinoma. Three‐year OS was 81% and 66% in HPV‐positive versus HPV‐negative patients with oropharyngeal carcinoma.ConclusionConcomitant weekly cisplatin 40 mg/m2 with accelerated RT was well tolerated and treatment compliance was high. © 2015 Wiley Periodicals, Inc. Head Neck 38: E559–E565, 2016
Genetic deletion of melanin‐concentrating hormone neurons impairs hippocampal short‐term synaptic plasticity and hippocampal‐dependent forms of short‐term memory
Authors: Le Barillier Léa , Léger Lucienne , Luppi Pierre‐Hervé , Fort Patrice , Malleret Gaël , Salin Paul‐Antoine 
Published: March 2015

ABSTRACTThe cognitive role of melanin‐concentrating hormone (MCH) neurons, a neuronal population located in the mammalian postero‐lateral hypothalamus sending projections to all cortical areas, remains poorly understood. Mainly activated during paradoxical sleep (PS), MCH neurons have been implicated in sleep regulation. The genetic deletion of the only known MCH receptor in rodent leads to an impairment of hippocampal dependent forms of memory and to an alteration of hippocampal long‐term synaptic plasticity. By using MCH/ataxin3 mice, a genetic model characterized by a selective deletion of MCH neurons in the adult, we investigated the role of MCH neurons in hippocampal synaptic plasticity and hippocampal‐dependent forms of memory. MCH/ataxin3 mice exhibited a deficit in the early part of both long‐term potentiation and depression in the CA1 area of the hippocampus. Post‐tetanic potentiation (PTP) was diminished while synaptic depression induced by repetitive stimulation was enhanced suggesting an alteration of pre‐synaptic forms of short‐term plasticity in these mice. Behaviorally, MCH/ataxin3 mice spent more time and showed a higher level of hesitation as compared to their controls in performing a short‐term memory T‐maze task, displayed retardation in acquiring a reference memory task in a Morris water maze, and showed a habituation deficit in an open field task. Deletion of MCH neurons could thus alter spatial short‐term memory by impairing short‐term plasticity in the hippocampus. Altogether, these findings could provide a cellular mechanism by which PS may facilitate memory encoding. Via MCH neuron activation, PS could prepare the day's learning by increasing and modulating short‐term synaptic plasticity in the hippocampus. © 2015 Wiley Periodicals, Inc.
Simulating Groundwater Dynamics Using Feflow‐3D Groundwater Model Under Complex Irrigation and Drainage Network of Dryland Ecosystems of Central Asia
Authors: Khalid Awan Usman , Tischbein Bernhard , Martius Christopher 
Published: March 2015

AbstractSurface and groundwater resources are often conjunctively used to cope with water scarcity in irrigated agriculture. Farmers in the dryland ecosystems of central Asia also utilize shallow groundwater in addition to surface water withdrawn from rivers. This study modelled groundwater dynamics in an irrigation and drainage network in Khorezm region, Uzbekistan. The system, characterized by a vast, unlined channel network used to convey water mainly for flood irrigation and an open drainage system, is typical of Central Asian irrigated areas. Groundwater levels in the region are shallow—this contributes to crop water requirements but threatens crop production through secondary salinization. High losses during irrigation in fields and through the irrigation network are the main causes of these shallow groundwater levels. The main objective of this study was thus to simulate groundwater levels under improved irrigation efficiency scenarios. The FEFLOW‐3D model, applied in a case study to the water users’ association (WUA) of Shomakhulum in south‐west Khorezm, was used to quantify the impact of improved irrigation efficiency scenarios on groundwater dynamics. The modelled scenarios were: current irrigation efficiency (S‐A, our baseline), improved conveyance efficiency (S‐B), increased field application efficiency (S‐C), and improved conveyance and application efficiency (S‐D). Recharge rates were separately determined for six hydrological response units (differing in groundwater level and soil type) and introduced into the FEFLOW‐3D model. After successful model calibration (R2 = 0.94) and validation (R2 = 0.93), the simulations showed that improving irrigation efficiency under existing agro‐hydroclimatic conditions would lower groundwater levels from the baseline scenario (S‐A) in August (the peak irrigation period) on average by 12 cm in S‐B, 38 cm in S‐C and 44 cm in S‐D. Any interventions which would improve irrigation efficiency will lower the groundwater levels and hence policy makers should consider them and formulate the policy accordingly. Copyright © 2015 John Wiley & Sons, Ltd.
Molecular profiling of sepsis in mice using Fourier Transform Infrared Microspectroscopy
Authors: Gautam Rekha , Deobagkar‐Lele Mukta , Majumdar Shamik , Chandrasekar Bhagawat , Victor Emmanuel , Ahmed Syed Moiz , Wadhwa Nitin , Verma Taru , Kumar Srividya , Sundaresan Nagalingam Ravi , Umapathy Siva , Nandi Dipankar 
Published: March 2015

Sepsis is a life threatening condition resulting from a high burden of infection. It is a major health care problem and associated with inflammation, organ dysfunction and significant mortality. However, proper understanding and delineating the changes that occur during this complex condition remains a challenge. A comparative study involving intra‐peritoneal injection of BALB/c mice with Salmonella Typhimurium (infection), lipopolysaccharide (endotoxic shock) or thioglycollate (sterile peritonitis) was performed. The changes in organs and sera were profiled using immunological assays and Fourier Transform Infrared (FTIR) micro‐spectroscopy. There is a rapid rise in inflammatory cytokines accompanied with lowering of temperature, respiratory rate and glucose amounts in mice injected with S. Typhimurium or lipopolysaccharide. FTIR identifies distinct changes in liver and sera: decrease in glycogen and protein/lipid ratio and increase in DNA and cholesteryl esters. These changes were distinct from the pattern observed in mice treated with thioglycollate and the differences in the data obtained between the three models are discussed. The combination of FTIR spectroscopy and other biomarkers will be valuable in monitoring molecular changes during sepsis.
Intra‐peritoneal infection with high dose of Salmonella Typhimurium leads to rapid increase in inflammatory cytokines, e.g. Tnfα (A). FTIR analysis of liver (B) and sera (C) identifies several metabolic changes: glycogen, protein/lipid, cholesteryl esters and DNA.magnified imageIntra‐peritoneal infection with high dose of Salmonella Typhimurium leads to rapid increase in inflammatory cytokines, e.g. Tnfα (A). FTIR analysis of liver (B) and sera (C) identifies several metabolic changes: glycogen, protein/lipid, cholesteryl esters and DNA.
Matrigel coatings for Parylene sheath neural probes
Authors: Lee Curtis D. , Hara Seth A. , Yu Lawrence , Kuo Jonathan T. W. , Kim Brian J. , Hoang Tuan , Pikov Victor , Meng Ellis 
Published: March 2015

AbstractThe biologically derived hydrogel Matrigel (MG) was used to coat a Parylene‐based sheath intracortical electrode to act as a mechanical and biological buffer as well as a matrix for delivering bioactive molecules to modulate the cellular response and improve recording quality. MG was loaded with dexamethasone to reduce the immune response together with nerve growth factor (NGF) and brain‐derived neurotrophic factor (BDNF) to maintain neuronal density and encourage neuronal ingrowth toward electrodes within the sheath. Coating the Parylene sheath electrode with the loaded MG significantly improved the signal‐to‐noise ratio for neural events recorded from the motor cortex in rat for more than 3 months. Electron microscopy showed even coverage of both the Parylene substrate and the platinum recording electrodes. Electrochemical impedance spectroscopy (EIS) of coated electrodes in 1× phosphate‐buffered saline demonstrated low impedance required for recording neural signals. This result was confirmed by in vivo EIS data, showing significantly decreased impedance during the first week of recording. Dexamethasone, NGF, and BDNF loaded into MG were released within 1 day in 1× phosphate‐buffered saline. Although previous studies showed that MG loaded with either the immunosuppressant or the neurotrophic factor cocktail provided modest improvement in recording quality in a 1‐month in vivo study, the combination of these bioactive molecules did not improve the signal quality over coating probes with only MG in a 3‐month in vivo study. The MG coating may further improve recording quality by optimizing the in vivo release profile for the bioactive molecules. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 357–368, 2016.
TNFα Post‐Translationally Targets ZnT2 to Accumulate Zinc in Lysosomes
Authors: Hennigar Stephen R. , Kelleher Shannon L. 
Published: March 2015

AbstractMammary epithelial cells undergo widespread lysosomal‐mediated cell death (LCD) during early mammary gland involution. Recently, we demonstrated that tumor necrosis factor‐α (TNFα), a cytokine released during early involution, redistributes the zinc (Zn) transporter ZnT2 to accumulate Zn in lysosomes and activate LCD and involution. The objective of this study is to determine how TNFα retargets ZnT2 to lysosomes. We tested the hypothesis that TNFα signaling dephosphorylates ZnT2 to uncover a highly conserved dileucine motif (L294L) in the C‐terminus of ZnT2, allowing adaptor protein complex‐3 (AP‐3) to bind and traffic ZnT2 to lysosomes. Confocal micrographs showed that TNFα redistributed wild‐type (WT) ZnT2 from late endosomes (Pearson's coefficient = 0.202 ± 0.05 and 0.097 ± 0.03; P < 0.05) to lysosomes (0.292 ± 0.03 and 0.649 ± 0.03; P < 0.0001), which increased lysosomal Zn (P < 0.0001) and activated LCD (P < 0.0001) compared to untreated cells. Mutation of the dileucine motif (L294V) eliminated the ability of TNFα to redistribute ZnT2 from late endosomes to lysosomes, increase lysosomal Zn, or activate LCD. Moreover, TNFα increased (P < 0.05) AP‐3 binding to wt ZnT2 but not to L294V immunoprecipitates. Finally, using phospho‐ and dephospho‐mimetics of predicted phosphorylation sites (T281, T288, and S296), we found that dephosphorylated S296 was required to target ZnT2 to accumulate Zn in lysosomes and activate LCD. Our findings suggest that women with variation in the C‐terminus of ZnT2 may be at risk for inadequate involution and breast disease due the inability to traffic ZnT2 to lysosomes. J. Cell. Physiol. 230: 2345–2350, 2015. © 2015 Wiley Periodicals, Inc.
Multiple dose pharmacokinetics of inhaled loxapine in subjects on chronic, stable antipsychotic regimens
Authors: Spyker Daniel A. , Riesenberg Robert A. , Cassella James V. 
Published: March 2015

AbstractThis randomized, double‐blind, placebo‐controlled, parallel‐group study was to determine the pharmacokinetic characteristics, safety, and tolerability of multiple doses of inhaled loxapine aerosol in subjects on a stable, oral, chronic antipsychotic regimen. Loxapine was delivered by means of a unique thermally generated aerosol comprising drug particles of a size designed for deep lung delivery and absorption. Thirty‐two subjects were randomized 1:1:1:1 to receive inhaled loxapine (total doses of 15, 20, or 30 mg) or inhaled placebo administered in 3 divided doses, given 4 hours apart. Following inhalation, the median Tmax was 2 minutes, and concentrations declined to about half Cmax approximately 5 minutes later across the 3 dose levels. The dose proportionality across data from this study combined with data from the single‐dose study showed a slope (90%CI) of log AUCinf versus log dose of 0.818 (0.762–0.875) across the 8 doses (n = 60 subjects) studied, indicating reasonable dose proportionality. The most common adverse events were cough (3 of 32, 9%), sedation (3 of 32, 9%), and dysgeusia (2 of 32, 6%). The inhalation of multiple doses of inhaled loxapine were well tolerated in study subjects and provided a safe, well‐tolerated means for rapidly and reliably achieving therapeutic plasma concentrations of loxapine. ClinicalTrials.gov identifier: NCT00555412
The impact of career customization on work outcomes: Boundary conditions of manager support and employee age
Authors: Bal P. Matthijs , van Kleef Marco , Jansen Paul G. W. 
Published: March 2015

SummaryThe current paper investigated the longitudinal effects of mass career customization (MCC) on job attitudes and objective career outcomes of employees in a professional service firm in the Netherlands. On the basis of theory on individualization of career trajectories, it was expected that the possibility for employees to customize their careers would be positively related to their job attitudes and subsequent objective career success, as indicated by their levels of affective commitment, work engagement, and received salary and bonuses. However, these effects were expected to occur primarily under the combination of high manager support for implementation of career customization and, on the basis of lifespan theory, older workers, as customization fulfills their increased heterogeneous career preferences. A three‐wave longitudinal study largely showed support for the study hypotheses; the relation between MCC use and work engagement and subsequent career success was stronger for older workers who received support for MCC, whereas the relation between MCC use and commitment was negative for older workers who received low support. The study shows the benefits of career customization in organizations by showing the conditions under which these benefits will manifest. Copyright © 2015 John Wiley & Sons, Ltd.
Shamals and climate variability in the Northern Arabian/Persian Gulf from 1973 to 2012
Authors: Al Senafi Fahad , Anis Ayal 
Published: March 2015

ABSTRACTThis paper presents key results from analysis of surface meteorological observations collected in the Northern Arabian/Persian Gulf (N Gulf; Kuwait, Bahrain, and NE Saudi Arabia), which spans a 40‐years period (1973–2012). The first part of this study analyzes climate variability in the N Gulf, and relates them to teleconnection patterns (North Atlantic Oscillation, El Nino Southern Oscillation, and Indian Ocean Dipole). Results of the analysis indicate that during the study period the climate in the region experienced a general trend of increase in temperature (0.8°C), decrease in barometric pressure (1 mbar), reduction in humidity (6%), and decrease in visibility (9%). Significant correlations were found between the three teleconnection patterns and the meteorological conditions suggesting that seasonal variabilities in air temperature, barometric pressure, and precipitation are closely related to the teleconnection patterns. The second part of this study examines the 40‐year variability of Shamal events (strong NW winds that commonly generate significant dust storms). The data suggests that on average Shamal events occur at a rate of 10 events year–1 with 85% of the events occurring during the summer and winter. The number of these events has increased in the past 14 years of the study period. These events resulted in abrupt changes in meteorological conditions: an increase in wind speed of 2.7 m s–1, a decrease in visibility of 1.7 km, and reduction in humidity of 4.3%. Seasonal variations in temperature (an increase in temperature during summer of 0.8°C, and a decrease of 1.5°C during winter) and barometric pressure (a decrease in barometric pressure during summer of 0.6 mbar and an increase of 7.8 mbar during winter) were observed during Shamal events.
Cerebrospinal fluid Aβ42 levels and APP processing pathway genes in Parkinson's disease
Authors: Bekris Lynn M. , Tsuang Debby W. , Peskind Elaine R. , Yu Chang E. , Montine Thomas J. , Zhang Jing , Zabetian Cyrus P. , Leverenz James B. 
Published: March 2015

AbstractBackgroundOf recent interest is the finding that certain cerebrospinal fluid (CSF) biomarkers traditionally linked to Alzheimer's disease (AD), specifically amyloid beta protein (Aβ), are abnormal in PD CSF. The aim of this exploratory investigation was to determine whether genetic variation within the amyloid precursor protein (APP) processing pathway genes correlates with CSF Aβ42 levels in Parkinson's disease (PD).MethodsParkinson's disease (n = 86) and control (n = 161) DNA were genotyped for 19 regulatory region tagging single‐nucleotide polymorphisms (SNPs) within nine genes (APP, ADAM10, BACE1, BACE2, PSEN1, PSEN2, PEN2, NCSTN, and APH1B) involved in the cleavage of APP. The SNP genotypes were tested for their association with CSF biomarkers and PD risk while adjusting for age, sex, and APOE ɛ4 status.ResultsSignificant correlation with CSF Aβ42 levels in PD was observed for two SNPs, (APP rs466448 and APH1B rs2068143). Conversely, significant correlation with CSF Aβ42 levels in controls was observed for three SNPs (APP rs214484, rs2040273, and PSEN1 rs362344).ConclusionsIn addition, results of this exploratory investigation suggest that an APP SNP and an APH1B SNP are marginally associated with PD CSF Aβ42 levels in APOE ɛ4 noncarriers. Further hypotheses generated include that decreased CSF Aβ42 levels are in part driven by genetic variation in APP processing genes. Additional investigation into the relationship between these findings and clinical characteristics of PD, including cognitive impairment, compared with other neurodegenerative diseases, such as AD, are warranted. © 2015 International Parkinson and Movement Disorder Society
A g‐factor metric for k‐t SENSE and k‐t PCA based parallel imaging
Authors: Binter Christian , Ramb Rebecca , Jung Bernd , Kozerke Sebastian 
Published: March 2015

PurposeTo propose and validate a g‐factor formalism for k‐t SENSE, k‐t PCA and related k‐t methods for assessing SNR and temporal fidelity.MethodsAn analytical gxf‐factor formulation in the spatiotemporal frequency domain is derived, enabling assessment of noise and depiction fidelity in both the spatial and frequency domain. Using pseudoreplica analysis of cardiac cine data the gxf‐factor description is validated and example data are used to analyze the performance of k‐t methods for various parameter settings.ResultsAnalytical gxf‐factor maps were found to agree well with pseudoreplica analysis for 3x, 5x, and 7x k‐t SENSE and k‐t PCA. While k‐t SENSE resulted in lower average gxf values (gxavg) in static regions when compared with k‐t PCA, k‐t PCA yielded lower gxavg values in dynamic regions. Temporal transfer was better preserved with k‐t PCA for increasing undersampling factors.ConclusionThe proposed gxf‐factor and temporal transfer formalism allows assessing noise performance and temporal depiction fidelity of k‐t methods including k‐t SENSE and k‐t PCA. The framework enables quantitative comparison of different k‐t methods relative to frame‐by‐frame parallel imaging reconstruction. Magn Reson Med 75:562–571, 2016. © 2015 Wiley Periodicals, Inc.
31P RINEPT MRSI and VBM reveal alterations in brain aging associated with major depression
Authors: Biedermann Sarah V. , Weber‐Fahr Wolfgang , Demirakca Traute , Tunc‐Skarka Nuran , Hoerst Mareen , Henn Fritz , Sartorius Alexander , Ende Gabriele 
Published: March 2015

PurposePhosphomono‐ and diesters, the major components of the choline peak in 1H magnetic resonance spectroscopy, are associated with membrane anabolic and catabolic mechanisms. With the refocused insensitive nuclei‐enhanced polarization transfer technique, these phospholipids are edited and enhanced in the 31P MR spectrum. In depressed patients, alterations of the choline peak and cerebral volume have been found, indicating a possible relation. Thus, combining MR phosphorous spectroscopy and volumetry in depressed patients seems to be a promising approach to detect underlying pathomechanisms.MethodsDepressed in‐patients were either treated with antidepressive medication or with electroconvulsive therapy and compared to matched healthy controls. 31P magnetic resonance spectroscopy imaging was conducted before and after the treatment phases. A 3D MRI dataset for volumetry was acquired in a dedicated 1H head coil.ResultsPhosphocholine and phosphoethanolamine were increased in depressed patients. Though patients responded to the treatments, phospholipids were not significantly altered. An increased age‐related gray matter loss in fronto‐limbic regions along with an altered relation of phosphomonoesters/phosphodiesters with age were found in depressed patients.DiscussionThe findings of increased phosphomonoesthers and an age*group interaction for gray matter volumes need further research to define the role of phospholipids in major depression and possible associations to gray matter loss. Magn Reson Med 73:1390–1400, 2015. © 2014 Wiley Periodicals, Inc.