bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular NAD+ response to post-hepatectomy liver failure: bridging preclinical and clinical findings
Authors: Kamali Can , Brunnbauer Philipp , Kamali Kaan , Saqr Al-Hussein Ahmed , Arnold Alexander , Harman Kamali Gulcin , Babigian Julia , Keshi Eriselda , Mohr Raphael , Felsenstein Matthäus , Moosburner Simon , Hillebrandt Karl-Herbert , Bartels Jasmin , Sauer Igor Maximilian , Tacke Frank , Schmelzle Moritz , Pratschke Johann , Krenzien Felix 
Published: August 2024

AbstractLiver fibrosis progressing to cirrhosis is a major risk factor for liver cancer, impacting surgical treatment and survival. Our study focuses on the role of extracellular nicotinamide adenine dinucleotide (eNAD+) in liver fibrosis, analyzing liver disease patients undergoing surgery. Additionally, we explore NAD+’s therapeutic potential in a mouse model of extended liver resection and in vitro using 3D hepatocyte spheroids. eNAD+correlated with aspartate transaminase (AST) and bilirubin after liver resection (AST:r = 0.2828,p = 0.0087; Bilirubin:r = 0.2584,p = 0.0176). Concordantly, post-hepatectomy liver failure (PHLF) was associated with higher eNAD+peaks (n = 10;p = 0.0063). Post-operative eNAD+levels decreased significantly (p < 0.05), but in advanced stages of liver fibrosis or cirrhosis, this decline not only diminished but actually showed a trend towards an increase. The expression of NAD+biosynthesis rate-limiting enzymes, nicotinamide phosphoribosyltransferase (NAMPT) and nicotinamide mononucleotide adenylyltransferase 3 (NMNAT3), were upregulated significantly in the liver tissue of patients with higher liver fibrosis stages (p < 0.0001). Finally, the administration of NAD+in a 3D hepatocyte spheroid model rescued hepatocytes from TNFalpha-induced cell death and improved viability (p < 0.0001). In a mouse model of extended liver resection, NAD+treatment significantly improved survival (p = 0.0158) and liver regeneration (p = 0.0186). Our findings reveal that eNAD+was upregulated in PHLF, and rate-limiting enzymes of NAD+biosynthesis demonstrated higher expressions under liver fibrosis. Further, eNAD+administration improved survival after extended liver resection in mice and enhanced hepatocyte viability in vitro. These insights may offer a potential target for future therapies.
Integrative, segregative, and degenerate harmonics of the structural connectome
Authors: Sipes Benjamin S. , Nagarajan Srikantan S. , Raj Ashish 
Published: August 2024

AbstractUnifying integration and segregation in the brain has been a fundamental puzzle in neuroscience ever since the conception of the “binding problem.” Here, we introduce a framework that places integration and segregation within a continuum based on a fundamental property of the brain–its structural connectivity graph Laplacian harmonics and a new feature we term the gap-spectrum. This framework organizes harmonics into three regimes–integrative, segregative, and degenerate–that together account for various group-level properties. Integrative and segregative harmonics occupy the ends of the continuum, and they share properties such as reproducibility across individuals, stability to perturbation, and involve “bottom-up” sensory networks. Degenerate harmonics are in the middle of the continuum, and they are subject-specific, flexible, and involve “top-down” networks. The proposed framework accommodates inter-subject variation, sensitivity to changes, and structure-function coupling in ways that offer promising avenues for studying cognition and consciousness in the brain.
Spatial control of the APC/C ensures the rapid degradation of cyclin B1
Authors: Cirillo Luca , Young Rose , Veerapathiran Sapthaswaran , Roberti Annalisa , Martin Molly , Abubacar Azzah , Perosa Camilla , Coates Catherine , Muhammad Reyhan , Roumeliotis Theodoros I , Choudhary Jyoti S , Alfieri Claudio , Pines Jonathon 
Published: August 2024

AbstractThe proper control of mitosis depends on the ubiquitin-mediated degradation of the right mitotic regulator at the right time. This is effected by the Anaphase Promoting Complex/Cyclosome (APC/C) ubiquitin ligase that is regulated by the Spindle Assembly Checkpoint (SAC). The SAC prevents the APC/C from recognising Cyclin B1, the essential anaphase and cytokinesis inhibitor, until all chromosomes are attached to the spindle. Once chromosomes are attached, Cyclin B1 is rapidly degraded to enable chromosome segregation and cytokinesis. We have a good understanding of how the SAC inhibits the APC/C, but relatively little is known about how the APC/C recognises Cyclin B1 as soon as the SAC is turned off. Here, by combining live-cell imaging, in vitro reconstitution biochemistry, and structural analysis by cryo-electron microscopy, we provide evidence that the rapid recognition of Cyclin B1 in metaphase requires spatial regulation of the APC/C. Using fluorescence cross-correlation spectroscopy, we find that Cyclin B1 and the APC/C primarily interact at the mitotic apparatus. We show that this is because Cyclin B1, like the APC/C, binds to nucleosomes, and identify an ‘arginine-anchor’ in the N-terminus as necessary and sufficient for binding to the nucleosome. Mutating the arginine anchor on Cyclin B1 reduces its interaction with the APC/C and delays its degradation: cells with the mutant, non-nucleosome-binding Cyclin B1 become aneuploid, demonstrating the physiological relevance of our findings. Together, our data demonstrate that mitotic chromosomes promote the efficient interaction between Cyclin B1 and the APC/C to ensure the timely degradation of Cyclin B1 and genomic stability.
Structure and replication of Pseudomonas aeruginosa phage JBD30
Authors: Valentová Lucie , Füzik Tibor , Nováček Jiří , Hlavenková Zuzana , Pospíšil Jakub , Plevka Pavel 
Published: August 2024

AbstractBacteriophages are the most abundant biological entities on Earth, but our understanding of many aspects of their lifecycles is still incomplete. Here, we have structurally analysed the infection cycle of the siphophage Casadabanvirus JBD30. Using its baseplate, JBD30 attaches to Pseudomonas aeruginosavia the bacterial type IV pilus, whose subsequent retraction brings the phage to the bacterial cell surface. Cryo-electron microscopy structures of the baseplate-pilus complex show that the tripod of baseplate receptor-binding proteins attaches to the outer bacterial membrane. The tripod and baseplate then open to release three copies of the tape-measure protein, an event that is followed by DNA ejection. JBD30 major capsid proteins assemble into procapsids, which expand by 7% in diameter upon filling with phage dsDNA. The DNA-filled heads are finally joined with 180-nm-long tails, which bend easily because flexible loops mediate contacts between the successive discs of major tail proteins. It is likely that the structural features and replication mechanisms described here are conserved among siphophages that utilize the type IV pili for initial cell attachment.
Evaluating Stuttering Self-Stigma and Its Relationship to Adverse Impact in Children and Adolescents With the Child Stuttering Self-Stigma Scale
Authors: Johnson Chelsea A. , Gerwin Katelyn L. , Tichenor Seth E. , Boyle Michael P. , Walsh Bridget 
Published: August 2024

Purpose:
Self-stigma occurs when a person internalizes and applies stereotypes, prejudice, and discrimination to themselves. For adults who stutter, self-stigma is linked to negative outcomes and reduced quality of life. The development of self-stigma in people who stutter is not well understood. The aim of this study is to evaluate stuttering self-stigma in school-age children and adolescents and explore potential relationships to stuttering's overall adverse impact.

Method:
One hundred one children and adolescents who stutter, aged 10–18 years, completed the Overall Assessment of the Speaker's Experience of Stuttering (OASES), a measure of adverse impact related to stuttering, and the Childhood Self-Stigma of Stuttering Scale (Child 4S), our novel adapted version of the Self-Stigma of Stuttering Scale (4S) created for this study. The Child 4S comprises three subscales measuring three stages of self-stigma: Awareness, Agreement, and Application. Each stage was evaluated for relationships with child age and the adverse impact of stuttering.

Results:
We found a range of self-stigma scores among children and adolescents who stutter. Child age did not correlate with Awareness and Agreement; however, older children and adolescents reported greater Application. All stages of self-stigma strongly predicted adverse impact as measured by the OASES, and latter stages of the model were stronger predictors than earlier stages.

Conclusions:
Children as young as 10 years old may experience stuttering self-stigma, and the application of self-stigma increases in adolescence, a critical period in the development of personal identity. Importantly, all stages of self-stigma predicted adverse impact related to stuttering, with latter stages being stronger predictors than earlier ones consistent with the progressive model of self-stigma being tested. The findings highlight the need for targeted, early intervention to mitigate downstream effects of stuttering self-stigma.

Supplemental Material:

https://doi.org/10.23641/asha.26352556
Doing the Inner and Outer Work: Culturally Responsive Practices in Gender-Affirming Voice Care
Authors: Vastine Wynde , Butcher Lisa 
Published: August 2024

Purpose:
This article aims to raise clinical awareness of (a) the barriers and forms of oppression that transgender and gender-expansive (TGE) people face, (b) the ways in which discrimination can subconsciously be enacted by practitioners and institutions, and (c) to provide actionable steps for providing gender-affirming voice care (GAVC) services.

Method:
This article draws on empirical evidence of inequitable access/barriers to resources and the heightened risk to well-being for TGE people, as well as the theoretical foundations from the field of psychology that lead to culturally responsive care of the voice. It clarifies and expands the tenets of gender-affirming practice and trauma-informed care and identifies action steps for practical application in this specialized area of voice. It highlights the need for greater self-reflection and ongoing education when providing GAVC services, particularly as it relates to the practitioner's understanding of the intersections of racism, transphobia, neurodivergence, and disability justice.

Conclusions:
In addition to having strong voice skills, practitioners who are lifelong learners, remain pliable to learning new ideas, and have a strong and sincere desire to provide GAVC are needed to work with this population. Those providing GAVC are recommended to first deepen their self-reflection skills and understanding of their own identities, as well as acquire a humble understanding of the 2SLGBTQIA+ community, especially in terms of the intersections of race and gender expression. The authors recommend personal and professional work through a justice, equity, diversity, and inclusion (JEDI) journey. This includes increasing one's counseling skills in order to provide tailored support that is specific to the client's needs. This article aims to provide a concrete entryway for the practice of gender-affirming care as it applies to the voice, while acknowledging that maintaining ethical gender-affirming practices requires a lifelong commitment to continuing education.
Clinical Accommodations for Children With Co-Occurring Developmental Language Disorder and Attention-Deficit/Hyperactivity Disorder
Authors: Manley Natalie R. , Wilder Amy 
Published: August 2024

Purpose:
Developmental language disorder (DLD) and attention-deficit/hyperactivity disorder (ADHD) are two of the most prevalent disorders in school-age children and are likely to be co-occurring. However, speech-language pathologists (SLPs) may receive limited training regarding strategies for capitalizing on strengths and accommodating the needs of children with ADHD relating to challenges in attention, working memory, behavioral inhibition, and self-regulation in the clinical setting. Therefore, in this tutorial, we describe how the cognitive profile of children with DLD and ADHD may present in the therapy room and review strategies for adapting therapeutic approaches to fit the needs of children with ADHD neurotypes.

Method:
This tutorial includes a review of research related to linguistic, cognitive, and behavioral differences in children with co-occurring DLD and ADHD. Strategies for accommodations in the therapy room are discussed, and we present a case study to demonstrate how these accommodations can be implemented.

Conclusions:
SLPs can enhance the effectiveness of therapy for children with co-occurring DLD and ADHD with an awareness of the struggles that children with DLD and ADHD experience in the therapy room. Providing these children with the appropriate accommodations can alleviate difficulties due to neurodivergent traits related to working memory, sustaining and shifting attention, social–emotional perception, behavioral inhibition, and self-regulation that occur along with linguistic difficulties.
Long‐term changes of wind resources and its impact on wind power development under climate change in China
Authors: Feng Shuanglei , Song Zongpeng , Yang Qing , Hou Yunhe , Wang Zheng , Liu Feng , Wang Bo , Wang Weisheng 
Published: August 2024

AbstractThe development of wind energy is indispensable in the pursuit of global carbon neutrality. This article's analysis of observational data across China reveals the annual average wind speed declined at a rate of −0.167 m · s−1 decade−1 between 1981 and 2014. This rate is 33 times faster than projections from the Coupled Model Intercomparison Project (CMIP) of the World Climate Research Programme. We propose a novel wind power scale estimation method based on annual average wind speed, suitable for assessing climate change impacts. Considering China's planned wind power generation in 2030, climate change may increase the required wind installed capacity by over 25% under the observed trend scenario. In contrast, historical average and CMIP scenarios could substantially overestimate wind potential while underestimating the necessary future wind power development scale. Climate change poses potential adverse impacts on China's carbon peak goals, necessitating targeted measures to mitigate these effects.
Analysis for integrated energy system: Benchmarking methods and implementation
Authors: Zhang Suhan , Gu Wei , Zhang X.‐P. , Chung C. Y. , Yu Ruizhi , Lu Shuai , Palma‐Behnke Rodrigo 
Published: August 2024

AbstractThe selection of suitable models and solutions is a fundamental requirement for conducting energy flow analysis in integrated energy systems (IES). However, this task is challenging due to the vast number of existing models and solutions, making it difficult to comprehensively compare scholars' studies with current work. In this paper, we aim to address this issue by presenting a comprehensive overview of mainstream IES models and clarifying their relationships, thereby providing guidance for scholars in selecting appropriate models. Additionally, we introduce several widely used solvers for solving algebraic and differential equations, along with their detailed implementations in the energy flow analysis of IES. Furthermore, we conduct extensive testing and demonstration of these models and methods in various cases to establish benchmarking datasets. To facilitate reproducibility, verification and comparisons, we provide open‐source access to these datasets, including system data, analysis settings and implementations of the various solvers in the mainstream models. Scholars can utilise the provided datasets to reproduce the results, verify the findings and perform comparative analyses. Moreover, they have the flexibility to customise these settings according to their specific requirements.
Deciphering the electricity–carbon market nexus: Challenges and prospects of electricity–carbon coupling
Authors: Zhang Ran , Lu Zelong , Shahidehpour Mohammad , Li Zuyi , Yan Lei 
Published: August 2024

AbstractThe power industry's carbon emissions stand out as a primary contributor to the overall carbon dioxide emissions within the energy system under the context of energy Internet. Thus, reducing emissions in the power sector has become crucial for achieving carbon neutrality. However, challenges from the electricity–carbon nexus have surfaced in effectively coordinating and integrating the carbon market with the electricity market. This paper initiates the exploration of such nexus by analysing the current status of major carbon emission trading markets on a global scale. Subsequently, it delves into a comprehensive examination of the coupling between the electricity and carbon markets across three levels: market participants, operational models and market mechanisms. Four key issues are then identified in the electricity–carbon nexus: challenges in decision‐making for market participants, discrepancies in operational timelines, the intricate design of market coupling mechanisms and the spillover effects of market risks. To tackle the above challenges in the electricity–carbon nexus, this paper takes a deep dive into two different models in understanding the nexus including econometric/statistical models and optimisation models, serving as the foundation for understanding the intricacies of electricity–carbon market coupling. This paper concludes with a detailed exploration for future roadmap and research prospects in the electricity–carbon market nexus.
The application and challenge of energy router in energy internet
Authors: Huang Jinliang , Han Wei , Tian Hanlei , Qiu Jie , Chan C. C. 
Published: August 2024

AbstractThe energy internet has emerged as a promising area of research in power systems with distributed generation. Similar to an internet router to connect and switch networks, the energy router within the energy internet plays a crucial role to integrate and distribute the energy flow. This paper provides an overview of the application and challenges associated with energy routers in the energy internet. Firstly, the ability of energy routers to bring together multiple energy sources and information is highlighted. Secondly, the discrepancies in power structure when energy routers are utilised in different voltage‐level power grids are discussed. Besides, the roles, structures, and design requirements of energy routers and how they interconnect with the power system, as well as some practical examples of energy router are delved. Finally, the main challenges faced by energy routers are summarised.
Joint chance‐constrained coordinated scheduling for electricity‐heat coupled systems considering hydrogen storage
Authors: Yang Lun , Sun Xunhang , Cao Xiaoyu , Chen Mengxiao , Guan Xiaohong 
Published: August 2024

AbstractStrong interdependence between power production and heat supply from combined power and heat units could restrict the wind power integration. Deploying hydrogen storage, typically formed by the electrolyser, hydrogen tank, and fuel cell, could be a promising measure to accommodate surplus wind power and facilitate the coordinated operation of power and heat. The authors consider coordinated scheduling under wind power uncertainty for an electricity‐heat coupled system with hydrogen storage and propose a distributionally robust joint chance‐constrained coordinated scheduling (DRJCC‐CS) model, where the waste heat from the electrolyser and fuel cell is incorporated. The authors design distributionally robust joint chance constraints to account for wind power uncertainty related constraints and derive their tractable inner and outer mixed‐integer convex approximations. Consequently, the proposed DRJCC‐CS model is casted into a mixed‐integer convex programme. Case studies are conducted to demonstrate the effectiveness of the proposed DRJCC‐CS method.
An improved kT/C noise cancellation technique with presampling for high‐speed SAR ADCs
Authors: Gu Yuanfan , Wang Kaipeng , Yi Tiange , Chen Xiaoguo , Yang Shiheng , Liu Jiaxin 
Published: August 2024

AbstractThe kT/C noise cancellation technique can effectively reduce the digital‐to‐analog converter (DAC) size for successive approximation register ADCs and thus relax the burden for input drivers and reference buffers. However, the prior kT/C noise cancellation technique suffers from a hard trade‐off between the noise, amplifier bandwidth and linearity. Here, an improved kT/C noise cancellation technique is proposed to break the trade‐off. It uses presampling to hold the input signal unchanged during the noise cancelling phase, leading to significantly relaxed requirements on the bandwidth and linearity of the amplifier. The proposed technique is verified in a 14‐bit 200 MS/s SAR analog‐to‐digital converter (ADC) with a DAC size of 128 fF. Simulation results show that this work achieves 75.6 dB signal to noise and distortion ratio and consumes 1.56 mW power.
A series‐fed frequency scanning antenna for millimeter wave wireless communication
Authors: Liu Lu , Luo Feixiang , Zhang Liang , Wang Yan 
Published: August 2024

AbstractIn the realms of 5G and the upcoming 6G communications, the millimeter‐wave bands offer substantially greater bandwidth. However, due to their propagation characteristics, aligning antennas becomes a challenging issue, particularly at the node side in industrial and vehicular network applications. Angle‐scanning antennas can significantly enhance communication reliability or provide new possibilities for spatial multiplexing. Previous studies have predominantly concentrated on frequency‐scanning antennas characterized by either low‐gain individual units or expensive digital arrays. Consequently, there has been a scarcity of solutions that are both cost‐effective and high‐gain. In this study, a method is proposed for achieving frequency scanning by extending the feed lines between elements. Based on this approach, a millimeter‐wave high‐gain serial‐feed frequency‐scanning antenna is developed. This antenna operates within the 60–64 GHz millimeter‐wave ISM (Industrial, Scientific, and Medical) band and is capable of performing a 10 scan in the E‐plane. Moreover, the antenna maintains a width of less than half a wavelength, allowing for the assembly of multiple antennas into a MIMO array.
A tumour‐associated macrophage‐based signature for deciphering prognosis and immunotherapy response in prostate cancer
Authors: Wang Jian , Guo Tao , Mi Yuanyuan , Meng Xiangyu , Xu Shuang , Dai Feng , Sun Chengwen , Huang Yi , Wang Jun , Zhu Lijie , Hou Jianquan , Wu Sheng 
Published: August 2024

AbstractFor the multistage progression of prostate cancer (PCa) and resistance to immunotherapy, tumour‐associated macrophage is an essential contributor. Although immunotherapy is an important and promising treatment modality for cancer, most patients with PCa are not responsive towards it. In addition to exploring new therapeutic targets, it is imperative to identify highly immunotherapy‐sensitive individuals. This research aimed to establish a signature risk model, which derived from the macrophage, to assess immunotherapeutic responses and predict prognosis. Data from the UCSC‐XENA, GEO and TISCH databases were extracted for analysis. Based on both single‐cell datasets and bulk transcriptome profiles, a macrophage‐related score (MRS) consisting of the 10‐gene panel was constructed using the gene set variation analysis. MRS was highly correlated with hypoxia, angiogenesis, and epithelial‐mesenchymal transition, suggesting its potential as a risk indicator. Moreover, poor immunotherapy responses and worse prognostic performance were observed in the high‐MRS group of various immunotherapy cohorts. Additionally, APOE, one of the constituent genes of the MRS, affected the polarisation of macrophages. In particular, the reduced level of M2 macrophage and tumour progression suppression were observed in PCa xenografts which implanted in Apolipoprotein E‐knockout mice. The constructed MRS has the potential as a robust prognostic prediction tool, and can aid in the treatment selection of PCa, especially immunotherapy options.