bluuebunny/crossref_metadata_2025_split huggingface dataset:
ENPP1 enzyme replacement therapy improves ectopic calcification but does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia
Authors: Reichenberger Ernst J , O’Brien Kevin , Hatori Ayano , Carpenter Thomas O , van de Wetering Koen , Flaman Lisa , Howe Jennifer , Ortiz Daniel , Sabbagh Yves , Chen I-Ping 
Published: August 2024

Abstract
Craniometaphyseal dysplasia (CMD) is a rare genetic bone disorder, characterized by progressive thickening of craniofacial bones and flared metaphyses of long bones. Craniofacial hyperostosis leads to the obstruction of neural foramina and neurological symptoms such as facial palsy, blindness, deafness, or severe headache. Mutations in ANKH (mouse ortholog ANK), a transporter of small molecules such as citrate and ATP, are responsible for autosomal dominant CMD. Knock-in (KI) mice carrying an ANKF377del mutation (AnkKI/KI) replicate many features of human CMD. Pyrophosphate (PPi) levels in plasma are significantly reduced in AnkKI/KI mice. PPi is a potent inhibitor of mineralization. To examine the extent to which restoration of circulating PPi levels may prevent the development of a CMD-like phenotype, we treated AnkKI/KI mice with the recombinant human ENPP1-Fc protein IMA2a. ENPP1 hydrolyzes ATP into AMP and PPi. Male and female Ank+/+ and AnkKI/KI mice (n ≥ 6/group) were subcutaneously injected with IMA2a or vehicle weekly for 12 wk, starting at the age of 1 wk. Plasma ENPP1 activity significantly increased in AnkKI/KI mice injected with IMA2a (Vehicle/IMA2a: 28.15 ± 1.65/482.7 ± 331.2 mOD/min; p &lt;.01), which resulted in the successful restoration of plasma PPi levels (Ank+/+/AnkKI/KI vehicle treatment/AnkKI/KI IMA2a: 0.94 ± 0.5/0.43 ± 0.2/1.29 ± 0.8 μM; p &lt;.01). We examined the skeletal phenotype by X-Ray imaging and μCT. IMA2a treatment of AnkKI/KI mice did not significantly correct CMD features such as the abnormal shape of femurs, increased bone mass of mandibles, hyperostotic craniofacial bones, or the narrowed foramen magnum. However, μCT imaging showed ectopic calcification near basioccipital bones at the level of the foramen magnum and on joints of AnkKI/KI mice. Interestingly, IMA2a treatment significantly reduced the volume of calcified nodules at both sites. Our data demonstrate that IMA2a is sufficient to restore plasma PPi levels and reduce ectopic calcification but fails to rescue skeletal abnormalities in AnkKI/KI mice under our treatment conditions.
Impact of integrating pest and pollinator management training on knowledge, perceptions, and livelihoods of avocado farmers in Kenya
Authors: Muriithi Beatrice W , Dubois Thomas , Kirui Leonard , Lattorff H Michael G , Mohamed Samira , Abdel-Rahman Elfatih M , Adan Marian , Kidoido Michael , Kassie Menale 
Published: August 2024

Abstract
Synthetic pesticides reduce pest populations but also beneficial organisms like pollinators, thereby threatening the productivity of pollination-dependent crops such as avocados. Through the novel concept of integrated pest and pollinator management (IPPM), the negative effects of pest control on pollinators are mitigated, leading to synergistic benefits for crop yield and quality. In this study, we investigated the impact of IPPM training on farmers’ knowledge, attitude, and management of avocado pests, changes in the size of avocado orchards, avocado losses due to insect pests, expenditure on pesticides, and income. We compared farmers using IPPM against those using each component in isolation (integrated pest management (IPM) or pollination services (PS) through beehive supplementation (PS)) and a control group that used conventional practices without PS. We utilized 2 rounds of panel data obtained from avocado growers from Murang’a County, Kenya, and employed difference-in-difference (DiD) and multinomial logistic regression models. The results indicate that individuals utilizing IPM demonstrated notable enhancements in their perceptions of avocado pests, pollinators, and IPPM packages in comparison to the control group. Conversely, those utilizing IPPM employed more effective pest management strategies than users of IPM only. In contrast to using IPM and PS in isolation, farmers who utilized IPPM registered a significantly higher (9.6%) increase in the proportion of household annual income from avocado farming compared to the control group. Our findings further show that farmers who received training were more likely to adopt IPM, PS, and IPPM practices. We recommend wide promotion of IPPM to enhance avocado production and livelihoods of smallholders in sub-Saharan Africa.
Navigating virtual realities: identifying barriers and facilitators to implementing VR-enhanced PT for youth with chronic pain
Authors: Jehl Nicole M , Hess Courtney W , Choate Ellison S , Nguyen Hannah T , Yang Yerin , Simons Laura E 
Published: August 2024

Abstract

Objective
Virtual reality (VR) can enhance engagement in outpatient physical therapy (PT) through distraction and gamification of movement. This study assessed barriers and facilitators to VR-enhanced PT.

Method
Data were collected during a feasibility trial of VR-enhanced PT for youth with chronic musculoskeletal pain. Semistructured and informal interviews were conducted with youth participants, their caregivers, and collaborating physical therapists. To analyze transcriptions, content analysis was employed in multiple rounds. Barriers and facilitators to VR implementation were coded using a deductive approach, then an inductive approach was used to identify emergent themes within each deductive code category.

Results
We completed interviews with youth participants (n = 9), caregivers (n = 7), and clinician stakeholders (n = 5). Coded barriers included: (1) participant identity and self-narrative inconsistent with the intervention, (2) system-level, structural constraints of healthcare, (3) lack of guidance and leadership from clinicians around VR use, (4) research burnout, (5) expectation violation and disappointment, and (6) missing the optimal treatment window. Coded facilitators included: (1) viewing VR as a bridge to achieving treatment goals, (2) having access to resources, (3) sustained positive experience and immersion in the game, (4) alignment between identity and the intervention, and (5) champion-level collaborations.

Conclusions
This study highlights the importance of considering the VR technology, person using the VR, and the context in which VR is being implemented to optimize uptake and acceptability. Adopting an implementation science lens to the field of VR for chronic pain will enhance the applicability and scale of impact.
Triple case report of persistent sciatic artery in Ethiopia: a rare vascular anomaly
Authors: Assefa Rahel Abebayehu , Zeleke Henok T/Silassie , Aboye Azmera Gissila 
Published: August 2024

Abstract
Persistent sciatic artery (PSA) is a rare congenital vascular anomaly resulting from embryologic axial artery malformation in the lower limb. This case report presents three patients aged 45–60, each with bilateral PSA presenting with symptoms indicative of PSA complications, including aneurysmal degeneration, limb ischemia, thromboembolism, or neuralgia from nerve compression. It highlights the diagnostic process, management strategies, and clinical outcomes observed at a tertiary referral hospital. Treatment involved a collaborative, multidisciplinary approach with vascular surgeons, internists, and radiologists tailoring interventions to individual patient findings and disease progression. This report aims to provide insights into the diverse presentations and management of PSA in a resource limited setting, encouraging further reporting and case studies to enhance understanding of therapeutic outcomes.
Gastropericardial fistula as a late complication of Roux-en-Y procedure
Authors: Bing Eric M , Skogsburg Amanda , Hayes Douglas 
Published: August 2024

Abstract
Pneumopericardium secondary to gastro-pericardial fistula is a rare complication associated with various surgical procedures and conditions, notably Roux-en-Y gastric bypass. This condition poses a risk of cardiac tamponade and can be fatal if not promptly diagnosed and managed. We present a case of a 62-year-old female with a history of gastric bypass who presented with nonspecific symptoms and was eventually diagnosed with pneumopericardium secondary to gastro-pericardial fistula. Despite efforts for timely intervention, including transfer to a facility with cardiothoracic surgery availability, the patient’s unstable condition precluded surgical intervention, leading to her eventual demise. A literature review reveals that the average time from Roux-en-Y gastric bypass surgery to presentation is nine years. The elusive nature of the presentation underscores the importance of a comprehensive clinical history in identifying this condition early. Awareness of gastro-pericardial fistula as a potential late complication of gastric bypass is crucial for timely diagnosis and intervention to improve patient outcomes.
An unusual presentation of sporadic dermal cylindroma and metacarpal enchondroma: case report and literature review
Authors: Alhazmi Bushra , Alyamani Anas , Alsalman Mohammed , Shokor Nada , Almeshal Obaid 
Published: August 2024

Abstract
Herein, we report an unusual presentation of a 38-year-old heathy lady with a painless slowly growing lesion in the volar aspect of her left distal forearm reaching the thenar area for &gt;15 years. Magnetic resonance imaging of the lesion showed multilobulated soft tissue mass with low and high signal in T1- and T2-weighted images with homogenous contrast enhancement. Additionally, a stable serpiginous bony lesion was noted in the first metacarpal bone showing low T1 and high T2 signals with peripheral enhancement. Histopathological examination was consistent with cylindroma and enchondroma, respectively. The patient had full recovery with no recurrence at a 1-year follow-up visit. While enchondroma is the most common benign tumor of the hand, sporadic dermal cylindroma, in contrast, rarely affects the upper extremity. The coexistence of these two pathological entities was not previously reported and it may complicate the diagnosis.
Open treatment of infected abdominal aortic aneurysm stemming from perineal infection
Authors: Hendrix Andrew , Makani Ankur , Crafton Thomas , Graham Camille , Torres-Medina Carlos , Cicchillo Michael 
Published: August 2024

Abstract
Infected abdominal aortic aneurysms (AAAs) make up a small minority of AAAs yet are characterized by a high fatality rate, largely attributed to their increased risk of aneurysm rupture. This case details a rare presentation of a 56-year-old man that developed Proteus mirabilis bacteremia secondary to a perineal abscess and subsequently experienced a 3 cm growth of his previously stable AAA over an 8 day period. This case underscores the importance of maintaining a heightened suspicion for infected aortic aneurysms in sick patients and highlights the critical role of surgical management in achieving source control.
Cultivating Pacific Cocoa: Chinese Plantation Labor in Colonial Era Samoa and Vanuatu
Authors: Martínez Julia T 
Published: August 2024

Abstract
From the 1890s to the 1930s the cocoa plantations of the southwest Pacific relied on Pacific Islander and Asian indentured labor from China and Vietnam. In Samoa and the New Hebrides (now Vanuatu) white planters lobbied colonial administrations for permission to introduce Chinese under contract as indentured labor, a coercive and controversial labor system. This article points to the role of transimperial economic competition in encouraging the use of indentured labor, traversing German, French, and British colonial administrations in the Pacific. Cocoa cultivation, white planters argued, required more regular attention compared with sugar or copra, and Chinese indentured workers were deemed the most suitable to meet these requirements. Australian, New Zealand and British administrators in the Pacific, who were keenly aware of ongoing French indenture practices, lobbied to introduce and retain indentured labor. Without the perceived competitive edge of indentured labor, cocoa cultivation was considered unlikely to prosper. It was not until the mid-1930s that recruitment of Chinese indentured labor ended. It came at a time of international and Chinese calls for abolition of coerced labor but was also driven by anti-immigration and pro-white nationalist sentiments. Even after recruitment ceased, the New Zealand administration in Samoa obliged Chinese workers to remain on the cocoa plantations. While the French did repatriate Chinese recruits, they retained Vietnamese workers. Only after 1945 were indentured workers freed from the obligation to cultivate cocoa, and finally permitted to abandon the plantations.
SCI1, a flower regulator of cell proliferation, and its partners NtCDKG2 and NtRH35 interact with the splicing machinery
Authors: Pinoti Vitor F , Ferreira Pedro B , Strini Edward J , Lubini Greice , Thomé Vanessa , Cruz Joelma O , Aziani Rodrigo , Quiapim Andréa C , Pinto Andressa P A , Araujo Ana Paula U , De Paoli Henrique C , Pranchevicius Maria Cristina S , Goldman Maria Helena S 
Published: August 2024

Abstract
Successful plant reproduction depends on the adequate development of floral organs controlled by cell proliferation and other processes. The Stigma/style cell-cycle inhibitor 1 (SCI1) gene regulates cell proliferation and affects the final size of the female reproductive organ. To unravel the molecular mechanism exerted by Nicotiana tabacum SCI1 in cell proliferation control, we searched for its interaction partners through semi-in vivo pull-down experiments, uncovering a cyclin-dependent kinase, NtCDKG;2. Bimolecular fluorescence complementation and co-localization experiments showed that SCI1 interacts with NtCDKG;2 and its cognate NtCyclin L in nucleoli and splicing speckles. The screening of a yeast two-hybrid cDNA library using SCI1 as bait revealed a novel DEAD-box RNA helicase (NtRH35). Interaction between the NtCDKG;2–NtCyclin L complex and NtRH35 is also shown. Subcellular localization experiments showed that SCI1, NtRH35, and the NtCDKG;2–NtCyclin L complex associate with each other within splicing speckles. The yeast two-hybrid screening of NtCDKG;2 and NtRH35 identified the conserved spliceosome components U2aʹ, NF-κB activating protein (NKAP), and CACTIN. This work presents SCI1 and its interactors, the NtCDKG;2–NtCyclin L complex and NtRH35, as new spliceosome-associated proteins. Our findings reveal a network of interactions and indicate that SCI1 may regulate cell proliferation through the splicing process, providing new insights into the intricate molecular pathways governing plant development.
Situations Where Tribunals can Apply GPL Depending on the Applicable Law to Settle a Dispute
Authors: Dumberry Patrick 
Published: August 2024

Abstract
This chapter examines the legal grounds based on which a tribunal can apply general principles of law (GPL), depending on what applicable law is used to settle a dispute. International law should play an important role in all investor–State arbitration cases. In this context, any tribunal applying international law will necessarily have to take into account GPL. This is simply because GPL is one of the three formal sources of international law. In any event, there are good reasons to argue, at least in the context of State contracts and bilateral investment treaties (BITs), that GPL should be applied to all investment disputes independently of the question of the choice of law made by the parties.
How to Identify GPL and Transpose Them on the International Plane
Authors: Dumberry Patrick 
Published: August 2024

Abstract
This chapter focuses on how tribunals should identify general principles of law (GPL) grounded in States’ domestic laws and transpose them onto the international plane. This process involves three steps. The first step concerns the identification of a principle that is common to domestic legal orders. The chapter shows that a comparative analysis of domestic laws is the proper method of investigation and that this method does not require examining all laws, but rather to focus on the most representative legal systems. The second step (distillation) requires the investigator to find common features of a given principle under domestic laws while leaving aside national particularities and specificities existing under some of them. Finally, the chapter looks at a number of questions surrounding the third step of the analysis, which involves the adaptation and transposition of a principle into the international legal order.
Analysis of Specific General Principles of Law
Authors: Dumberry Patrick 
Published: August 2024

Abstract
This chapter assesses whether a number of concepts which have been identified in the past by scholars, courts, and tribunals should be recognized as general principles of law (GPL). These concepts include the clean hands doctrine; good faith; unjust enrichment; force majeure; proportionality; acquired rights; rebus sic stantibus; mitigation of damages; denial of justice and due process; and fair and equitable treatment (FET), including the specific question regarding the protection of ‘legitimate expectations’. The question of the GPL status of the concept of legitimate expectations is very controversial. So far, awards have simply not addressed this matter. Nevertheless, the concept of legitimate expectations is already considered by tribunals—except in the specific context of the North American Free Trade Agreement (NAFTA)—as an autonomous element of the FET standard.
Conclusion
Authors: Dumberry Patrick 
Published: August 2024

Abstract
This concluding chapter argues that tribunals should make use of general principles of law (GPL) in a manner that goes beyond the traditional functions that are typically identified in doctrine. Thus, GPL should not just be used as ‘gap-filling’ whenever treaty provisions or rules of customary international law do not provide a solution to a particular issue. They should also not be simply used to provide guidance for the interpretation and application of vague or uncertain terms contained in treaties. GPL could be used by tribunals to improve the outcome of decision making by achieving a better balance between investors' rights and States' public interests that is acceptable to all different stakeholders in the process. In other words, GPL should play an important and positive role in the process of recalibrating international investment law in reaction to the current backlash against the legitimacy of the system perceived by some as inherently favourable to the interests of foreign corporations.
Perfect proofs at first order
Authors: Tennant Neil 
Published: August 2024

Abstract
In this note we extend a remarkable result of Brauer (2024, Journal of Logic and Computation) concerning propositional Classical Core Logic. We show that it holds also at first order. This affords a soundness and completeness result for Classical Core Logic. The $\mathbb{C}^{+}$-provable sequents are exactly those that are uniform substitution instances of perfectly valid sequents, i.e. sequents that are valid and that need every one of their sentences in order to be so. Brauer (2020, Review of Symbolic Logic, 13, 436–457) showed that the notion of perfect validity itself is unaxiomatizable. In the Appendix we use his method to show that our notion of relevant validity in Tennant (2024, Philosophia Mathematica) is likewise unaxiomatizable. It would appear that the taking of substitution instances is an essential ingredient in the construction of a semantical relation of consequence that will be axiomatizable—and indeed, by the rules of proof for Classical Core Logic.
Irregular fixation – II. The orbits of irregular satellites
Authors: Grishin Evgeni 
Published: August 2024

ABSTRACT
Irregular satellites (ISs) are believed to have been captured during the Solar system’s dynamical history and provide clues for the Solar system’s formation and evolution. ISs occupy a large fraction of the Hill sphere of their host planet and their orbits are highly perturbed by the Sun. We use a novel formalism developed in Paper I to characterize their orbits in terms of an effective secular Hamiltonian (the Brown Hamiltonian) that accounts for their large orbital separations. We find that prograde satellites generally follow the Brown Hamiltonian, while retrograde satellites (which extend further) deviate more significantly. Nevertheless, the phase portrait is much better described by the Brown Hamiltonian for all satellites. We construct a semi-analytical criterion that predicts the librating orbit based on the effective energy due to the Brown Hamiltonian. We also check our results with highly accurate N-body integrations of satellite orbits, where initial conditions are loaded directly from the updated ephemeris from the NASA Horizons data base. Although the retrograde librating orbits occupy more area in the parameter space, the vast majority of librating ISs are prograde. Using our method, we find 13 librating satellites, 8 of them previously known to librate, and the rest shown to librate for the first time. Further observations of existing and new satellites could shed more light on the dynamical history of the Solar system and satellite formation and test our results.
Rotational excitation and de-excitation of the interstellar propargyl (H2CCCH+) cation by collisions with helium atoms
Authors: Mehnen Bilel , Hendaoui Hamza , Żuchowski Piotr 
Published: August 2024

ABSTRACT
The propargyl cation, H2CCCH+, is a crucial hydrocarbon precursor in the chemical evolution of the interstellar medium (ISM). It serves as a key intermediate for understanding ISM hydrocarbon chemistry. This work presents state-to-state collisional rate coefficients for rotational excitation and de-excitation of ortho- and para-H2CCCH+ colliding with He at ISM-relevant temperatures (T ≤ 60 K). We employed a high-level post-Hartree-Fock CCSD(T)-F12/aug-cc-pVTZ method to generate a 3D interaction potential energy surface (3D-PES) for the weakly bound H2CCCH+-He complex. The analytical 3D-PES was then used in close-coupling calculations to determine cross-sections for rotational excitation and de-excitation of ortho- and para-H2CCCH+ by collisions with He for kinetic energies (Ek) up to 300 cm−1. Inelastic collision rate coefficients were subsequently obtained by thermally averaging the cross-sections over a Maxwell–Boltzmann distribution of kinetic energies. Our results indicate that at very low temperatures (T &lt; 15 K), rotational transitions associated with Δj = Δkc = ±1, which are crucial for the identification of H2CCCH+ in astronomical surveys, exhibit the largest cross-sections. In contrast, at higher temperatures, transitions with Δj = Δkc = ±2 become more dominant. These data hold significant implications for the determination of the H2CCCH+ abundance in the ISM and improve the modelling of interstellar hydrocarbon-chain reaction pathways.