bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrative DNA methylation and gene expression analysis in high-grade soft tissue sarcomas
Authors: Renner Marcus , Wolf Thomas , Meyer Hannah , Hartmann Wolfgang , Penzel Roland , Ulrich Alexis , Lehner Burkhard , Hovestadt Volker , Czwan Esteban , Egerer Gerlinde , Schmitt Thomas , Alldinger Ingo , Renker Eva Kristin , Ehemann Volker , Eils Roland , Wardelmann Eva , Büttner Reinhard , Lichter Peter , Brors Benedikt , Schirmacher Peter , Mechtersheimer Gunhild 
Published: December 2013

Abstract
Background
High-grade soft tissue sarcomas are a heterogeneous, complex group of aggressive malignant tumors showing mesenchymal differentiation. Recently, soft tissue sarcomas have increasingly been classified on the basis of underlying genetic alterations; however, the role of aberrant DNA methylation in these tumors is not well understood and, consequently, the usefulness of methylation-based classification is unclear.

Results
We used the Infinium HumanMethylation27 platform to profile DNA methylation in 80 primary, untreated high-grade soft tissue sarcomas, representing eight relevant subtypes, two non-neoplastic fat samples and 14 representative sarcoma cell lines. The primary samples were partitioned into seven stable clusters. A classification algorithm identified 216 CpG sites, mapping to 246 genes, showing different degrees of DNA methylation between these seven groups. The differences between the clusters were best represented by a set of eight CpG sites located in the genes SPEG, NNAT, FBLN2, PYROXD2, ZNF217, COL14A1, DMRT2 and CDKN2A. By integrating DNA methylation and mRNA expression data, we identified 27 genes showing negative and three genes showing positive correlation. Compared with non-neoplastic fat, NNAT showed DNA hypomethylation and inverse gene expression in myxoid liposarcomas, and DNA hypermethylation and inverse gene expression in dedifferentiated and pleomorphic liposarcomas. Recovery of NNAT in a hypermethylated myxoid liposarcoma cell line decreased cell migration and viability.

Conclusions
Our analysis represents the first comprehensive integration of DNA methylation and transcriptional data in primary high-grade soft tissue sarcomas. We propose novel biomarkers and genes relevant for pathogenesis, including NNAT as a potential tumor suppressor in myxoid liposarcomas.
Intensive Chemotherapy for Childhood Acute Lymphoblastic Leukemia: Results of the Randomized Intercontinental Trial ALL IC-BFM 2002
Authors: Stary Jan , Zimmermann Martin , Campbell Myriam , Castillo Luis , Dibar Eduardo , Donska Svetlana , Gonzalez Alejandro , Izraeli Shai , Janic Dragana , Jazbec Janez , Konja Josip , Kaiserova Emilia , Kowalczyk Jerzy , Kovacs Gabor , Li Chi-Kong , Magyarosy Edina , Popa Alexander , Stark Batia , Jabali Yahia , Trka Jan , Hrusak Ondrej , Riehm Hansjörg , Masera Giuseppe , Schrappe Martin 
Published: December 2013

Purpose From 2002 to 2007, the International Berlin-Frankfurt-Münster Study Group conducted a prospective randomized clinical trial (ALL IC-BFM 2002) for the management of childhood acute lymphoblastic leukemia (ALL) in 15 countries on three continents. The aim of this trial was to explore the impact of differential delayed intensification (DI) on outcome in all risk groups. Patients and Methods For this trial, 5,060 eligible patients were divided into three risk groups according to age, WBC, early treatment response, and unfavorable genetic aberrations. DI was randomized as follows: standard risk (SR), two 4-week intensive elements (protocol III) versus one 7-week protocol II; intermediate risk (IR), protocol III × 3 versus protocol II × 1; high risk (HR), protocol III × 3 versus either protocol II × 2 (Associazione Italiana Ematologia Oncologia Pediatrica [AIEOP] option), or 3 HR blocks plus single protocol II (Berlin-Frankfurt-Münster [BFM] option). Results At 5 years, the probabilities of event-free survival and survival were 74% (± 1%) and 82% (± 1%) for all 5,060 eligible patients, 81% and 90% for the SR (n = 1,564), 75% and 83% for the IR (n = 2,650), and 55% and 62% for the HR (n = 846) groups, respectively. No improvement was accomplished by more intense and/or prolonged DI. Conclusion The ALL IC-BFM 2002 trial is a good example of international collaboration in pediatric oncology. A wide platform of countries able to run randomized studies in ALL has been established. Although the alternative DI did not improve outcome compared with standard treatment and the overall results are worse than those achieved by longer established leukemia groups, the national results have generally improved.
Effects of Exercise on Treatment-Related Adverse Effects for Patients With Prostate Cancer Receiving Androgen-Deprivation Therapy: A Systematic Review
Authors: Gardner Jason R. , Livingston Patricia M. , Fraser Steve F. 
Published: December 2013

Purpose Androgen-deprivation therapy is a commonly used treatment for men with prostate cancer; however, the adverse effects can be detrimental to patient health and quality of life. Exercise has been proposed as a strategy for ameliorating a range of these treatment-related adverse effects. We conducted a systematic review of the literature regarding the effects of exercise on treatment-related adverse effects in men receiving androgen-deprivation therapy for prostate cancer. Methods An online electronic search of the Cochrane Library, EMBASE, MEDLINE, CINAHL, SPORTDiscus, and Health Source databases was performed to identify relevant peer-reviewed articles published between January 1980 and June 2013. Eligible study designs included randomized controlled trials as well as uncontrolled trials with pre- and postintervention data. Information was extracted regarding participant and exercise intervention characteristics as well as the effects of exercise on bone health, body composition, physical performance, cardiometabolic risk, fatigue, and quality of life. Results Ten studies were included, with exercise interventions involving aerobic and/or resistance training. Exercise training demonstrated benefits in muscular strength, cardiorespiratory fitness, functional task performance, lean body mass, and fatigue, with inconsistent effects observed for adiposity. The impact of exercise on bone health, cardiometabolic risk markers, and quality of life are currently unclear. Conclusion Among patients with prostate cancer treated with androgen-deprivation therapy, appropriately prescribed exercise is safe and may ameliorate a range of treatment-induced adverse effects. Ongoing research of high methodologic quality is required to consolidate and expand on current knowledge and to allow the development of specific evidence-based exercise prescription recommendations.
Phantom Public
Authors: Fiel Wolfgang 
Published: December 2013

Abstract
In 2008 tat ort inhabited a flat for a sustained period of two months in the Siedlung an der Ach/Estate on the Ach, located in the small city of Bregenz in Vorarlberg, Austria. The project was the pilot to an open-ended series of self-organized artist residencies, with the aim of looking into the immediate living conditions of segregated, dense and highly conflict-laden social housing estates. This is an account of the theory and practice that formed a strategy to unlock a wealth of information that all too often is neglected by official bodies, governments or other representational organizations. By rendering this tremendous expertise visible, tat ort are able to reveal how a specific apparatus emerges, how to describe or interpret it adequately, and – if necessary – how to foster or reinvigorate its underlying dynamic with the catalysing spirit and playful dexterity of genuine artistic creation.
When art is a form of behaviour …
Authors: Aga B. 
Published: December 2013

Abstract
Roy Ascott’s influence is pervasive, through his pedagogy (the ‘Ground Course’ and the ‘Planetary Collegium’) and through the telematics works in the 1980s and 1990s. In the early 1960s, Ascott lit the slow burning fuse of a mind-bomb that, rather than shattering the fabric of the art world, slowly dissolved its materiality. As telematic shrapnel penetrate its vital organs, the object d’art staggers on, zombie like, curators wheeling over head, picking over the corpse. Rather than try to resuscitate the remains, there are those who prefer to surf the shockwave of this legacy in the hope that the future will catch up.
Focus on form as a joint accomplishment: An attempt to bridge the gap between focus on form research and conversation analytic research on SLA
Authors: Fasel Lauzon Virginie , Pekarek Doehler Simona 
Published: December 2013

Abstract

Several debates have recently addressed complementarities and/or (in)compatibilities between two lines of research concerned with second language interactions: Focus on Form research and conversation analytic work on repair in second language interactions. While our expertise primarily lies in the latter, we follow up on recent calls emanating from the former for more qualitatively oriented analysis. In this paper, we report on a study of correction in naturally occurring French L2 classroom interaction addressing the following question: how is attention focus on form distributed among the participants and interactionally organized across the temporal unfolding of talk? We show the analytic difficulty of determining precisely whose focus we observe in focus on form episodes. The findings substantiate an understanding of attention focus – along with the cognitive orientations of participants – as a process that is interactionally occasioned and organized, and the transformation of which into joint focus hinges on the local contingencies of talk.
Point of view in British Sign Language and spoken English narrative discourse: the example of “The Tortoise and the Hare”
Authors: Earis Helen , Cormier Kearsy 
Published: December 2013

AbstractThis paper discusses how point of view (POV) is expressed in British Sign Language (BSL) and spoken English narrative discourse. Spoken languages can mark changes in POV using strategies such as direct/indirect discourse, whereas signed languages can mark changes in POV in a unique way using “role shift”. Role shift is where the signer “becomes” a referent by taking on attributes of that referent, e.g. facial expression. In this study, two native BSL users and two native British English speakers were asked to tell the story “The Tortoise and the Hare”. The data were then compared to see how point of view is expressed and maintained in both languages. The results indicated that the spoken English users preferred the narrator's perspective, whereas the BSL users preferred a character's perspective. This suggests that spoken and signed language users may structure stories in different ways. However, some co-speech gestures and facial expressions used in the spoken English stories to denote characters' thoughts and feelings bear resemblance to the hand movements and facial expressions used by the BSL storytellers. This suggests that while approaches to storytelling may differ, both languages share some gestural resources which manifest themselves in different ways across different modalities.
One word at a time: Mental representations of object shape change incrementally during sentence processing
Authors: Sato Manami , Schafer Amy J. , Bergen Benjamin K. 
Published: December 2013

AbstractWe report on two experiments that ask when and under what linguistic conditions comprehenders construct detailed shape representations of mentioned objects, and whether these can change over the course of a sentence when new information contradicts earlier expectations. We used Japanese because the verb-final word order of Japanese presented a revealing test case where information about objects can radically change with a subsequent verb. The results show that language understanders consistently generate a distinct and detailed shape for an object by integrating the semantic contributions of different sentential elements. These results first confirm that the tendency to generate specific shape information about objects that are involved in described events is not limited to English, but is also present in Japanese, a typologically and genetically distinct language. But more importantly, they shed light on the processing mechanism of object representation, showing that mental representations are initiated sentence medially, and are rapidly revised if followed by a verb that implies a change to an object shape. This work contributes to ongoing research on incremental language processing – comprehenders appear to construct extremely detailed semantic representations early in a sentence, and modify them as needed.
Health Care Worker Exposures to Pertussis: Missed Opportunities for Prevention
Authors: Kuncio Danica E. , Middleton Maria , Cooney Mary G. , Ramos Mark , Coffin Susan E. , Feemster Kristen A. 
Published: December 2013

OBJECTIVE:
Pediatric health care workers (HCWs) are at particular risk for pertussis exposure, infection, and subsequent disease transmission to susceptible patients. This cross-sectional study describes the epidemiology of occupational exposures to pertussis and identifies factors that may inform interventions to promote effective implementation of infection prevention and control (IPC) guidelines.

METHODS:
We abstracted data from occupational health (OH) and IPC records for pertussis cases that resulted in an exposure investigation in a large quaternary pediatric care network, January 1, 2002 to July 18, 2011. We calculated the frequency of occupational exposures and measured associated characteristics. To assess the frequency of potential missed exposures, we reviewed electronic health record (EHR) data identifying laboratory-confirmed pertussis cases not documented in OH or IPC records.

RESULTS:
A total of 1193 confirmed HCW pertussis exposures were associated with 219 index cases during the study period. Of these, 38.8% were infants &lt;6 months old and 7 were HCWs. Most (77.5%) of exposures occurred in the emergency department or an ambulatory site; 27.0% of exposures occurred after documented initiation of IPC precautions. We identified 450 laboratory-confirmed pertussis cases through EHR review, of which 49.8% (N = 224) had no OH or IPC investigation. The majority of uninvestigated cases (77.2%) were from ambulatory sites.

CONCLUSIONS:
Occupational exposures to pertussis occur frequently in pediatric health care settings despite appropriate IPC guidelines. Interventions are needed to ensure consistent implementation of IPC practices and timely identification and reporting of pertussis index cases to prevent HCW exposures and potential transmission to patients.
Decreasing Hypothermia During Delivery Room Stabilization of Preterm Neonates
Authors: Pinheiro Joaquim M.B. , Furdon Susan A. , Boynton Susan , Dugan Robin , Reu-Donlon Christine , Jensen Sharon 
Published: December 2013

BACKGROUND AND OBJECTIVE:Hypothermia during delivery room stabilization of very low birth weight (VLBW) newborns is independently associated with mortality, yet it occurred frequently both in collaborative networks and at our institution. We aimed to attain admission temperatures in the target range of 36°C to 38°C in ≥90% of inborn VLBW neonates through implementation of a thermoregulation bundle.METHODS:This quality improvement project extended over 60 consecutive months, using sequential plan–do–check–act cycles. During the 14 baseline months, we standardized temperature measurements and developed the Operation Toasty Tot thermoregulation bundle (including consistent head and torso wrapping with plastic, warmed blankets, and a closed stabilization room). We introduced this bundle in month 15 and added servo-controlled, battery-powered radiant warmers for stabilization and transfer in month 21. We provided results and feedback to staff throughout, using simple graphics and control charts.RESULTS:There were 164 inborn VLBW babies before and 477 after bundle implementation. Introduction and optimization of the bundle decreased the incidence of hypothermia, with rates remaining in the target range for the last 13 study months. The incidence of temperatures &gt;38°C was ∼2% both before and after bundle implementation.CONCLUSIONS:This thermoregulation bundle resulted in sustained improvement in normothermia rates during delivery room stabilization of VLBW newborns. Our benchmark goal of ≥90% admission temperatures above 36°C was met without increasing hyperthermia rates. Because these results compare favorably with those of recently published research or improvement collaboratives, we aim to maintain our performance through routine surveillance of admission temperatures.
Acute and Chronic Effects of Sleep Duration on Blood Pressure
Authors: Au Chun Ting , Ho Crover Kwok Wah , Wing Yun Kwok , Lam Hugh Simon , Li Albert Martin 
Published: December 2013

OBJECTIVE:
To evaluate the association between ambulatory blood pressure (ABP) and sleep duration as measured by 7-day sleep diary and nocturnal polysomnography in normal-weight adolescents without significant obstructive sleep apnea.

METHODS:
Subjects aged 10 to 17.9 years with an obstructive apnea hypopnea index &lt;5 underwent polysomnography for 9.5 hours and 24-hour ABP monitoring commencing at noon on the same day. ABP was divided into prepolysomnography, in bed during polysomnography, and postpolysomnography periods for separate analyses. Sleep duration (SpD7) was obtained from a 7-day sleep diary, reflecting the sleep pattern in the week before admission. Total sleep time (TST) and sleep efficiency (SpE) were obtained from polysomnography.

RESULTS:
A total of 143 adolescents participated. SpD7 was inversely associated with systolic blood pressure (SBP) in prepolysomnography, in-bed, and postpolysomnography periods (all β = −2 mm Hg) and with diastolic blood pressure (DBP) in prepolysomnography and in-bed periods (all β = −1 mm Hg). TST was inversely associated with SBP in the postpolysomnography period (β = −1.5 mm Hg). SpE was inversely associated with SBP in in-bed period (β = −0.1 mm Hg) and with DBP in in-bed (β = −0.1 mm Hg) and postpolysomnography (β = −0.2 mm Hg) periods. Neither TST nor SpE was associated with SBP and DBP in prepolysomnography period.

CONCLUSIONS:
Short sleep duration as reflected by 7-day sleep diary was associated with higher blood pressure in normal-weight adolescents. Occasional adequate sleep may partially ameliorate the risk of high blood pressure but may not completely reverse the effect of long-term sleep insufficiency.
7% Hypertonic Saline in Acute Bronchiolitis: A Randomized Controlled Trial
Authors: Jacobs Jonathan D. , Foster Megan , Wan Jim , Pershad Jay 
Published: December 2013

BACKGROUND:
Research suggests that hypertonic saline (HS) may improve mucous flow in infants with acute bronchiolitis. Data suggest a trend favoring reduced length of hospital stay and improved pulmonary scores with increasing concentration of nebulized solution to 3% and 5% saline as compared with 0.9% saline mixed with epinephrine. To our knowledge, 7% HS has not been previously investigated.

METHODS:
We conducted a prospective, double-blind, randomized controlled trial in 101 infants presenting with moderate to severe acute bronchiolitis. Subjects received either 7% saline or 0.9% saline, both with epinephrine. Our primary outcome was a change in bronchiolitis severity score (BSS), obtained before and after treatment, and at the time of disposition from the emergency department (ED). Secondary outcomes measured were hospitalization rate, proportion of admitted patients discharged at 23 hours, and ED and inpatient length of stay.

RESULTS:
At baseline, study groups were similar in demographic and clinical characteristics. The decrease in mean BSS was not statistically significant between groups (2.6 vs 2.4 for HS and control groups, respectively). The difference between the groups in proportion of admitted patients (42% in HS versus 49% in normal saline), ED or inpatient length of stay, and proportion of admitted patients discharged at 23 hours was not statistically significant.

CONCLUSIONS:
In moderate to severe acute bronchiolitis, inhalation of 7% HS with epinephrine does not appear to confer any clinically significant decrease in BSS when compared with 0.9% saline with epinephrine.
Managing Downy Brome (Bromus tectorum) in the Central Rockies: Land Manager Perspectives
Authors: Kelley Windy K. , Fernandez-Gimenez Maria E. , Brown Cynthia S. 
Published: December 2013

AbstractSuccessful management of downy brome (also known as cheatgrass) requires understanding land managers' perceptions and decisions about whether to invest in its control. We investigated ranchers' and natural resource professionals' (NRPs) perceptions and knowledge about downy brome ecology and its impacts, their current downy brome management practices and satisfaction with those practices, and their information and technical needs using focus groups and a mail survey of ranchers and NRPs in Colorado and Wyoming. Both groups thought downy brome was a problem, and perception of the severity of downy brome corresponded to the level of infestation in the respondent's region. NRPs identified downy brome as a bigger problem than did ranchers from the same area in all but one region. Ranchers were most likely to use early spring grazing to control downy brome, and NRPs were most likely to use seeding, imazapic herbicide, or a combination of methods. Both groups reported that the primary constraint to controlling downy brome was that other weeds were a higher priority. Ranchers and NRPs wanted more information about the control methods they were already likely to use as well as other downy brome control methods. Our findings suggest that (1) listing a species as a noxious weed may provide an important incentive to control it, but trade-offs among control efforts for different species must be carefully considered; (2) managers need to know more about low-cost, low-labor strategies for managing downy brome; and (3) some managers need to be informed about how to identify downy brome, its potential negative effects, and how to prevent its spread. Better quantification of the economic and ecological impacts of downy brome in the Central Rocky Mountains, continued development of effective and economically viable management methods, and improvement in the dissemination of that information to land managers are necessary for successful control of downy brome.