bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic effect of angiotensin II on vascular endothelial growth factor-A-mediated differentiation of bone marrow-derived mesenchymal stem cells into endothelial cells
Authors: Ikhapoh Izuagie Attairu , Pelham Christopher J , Agrawal Devendra K 
Published: June 2015

Abstract
Introduction
Increased levels of angiotensin II (Ang II) and activity of Ang II receptor type 1 (AT1R) elicit detrimental effects in cardiovascular disease. However, the role of Ang II receptor type 2 (AT2R) remains poorly defined. Mesenchymal stem cells (MSCs) replenish and repair endothelial cells in the cardiovascular system. Herein, we investigated a novel role of angiotensin signaling in enhancing vascular endothelial growth factor (VEGF)-A-mediated differentiation of MSCs into endothelial cells (ECs).

Methods
Bone marrow was aspirated from the femurs of Yucatan microswine. MSCs were extracted via ficoll density centrifugation technique and were strongly immunopositive for MSC markers, CD44, CD90, and CD105, but negative for hematopoietic markers, CD14 and CD45. Subsequently, naïve MSCs were differentiated for 10 days in varying concentrations and combinations of VEGF-A, Ang II, and AT1R or AT2R antagonists. Markers specific to ECs were determined by FACS analysis.

Results
AT1R and AT2R expression and cellular localization was demonstrated in MSCs stimulated with VEGF-A and Ang II via quantitative RT-PCR and immunofluorescence, respectively. Differentiation of naïve MSCs in media containing Ang II (2 ng/ml) plus low-dose VEGF-A (2 ng/ml) produced a significantly higher percentage of cells that were positive for expression of EC markers (for example, platelet endothelial cell adhesion molecule, vascular endothelial Cadherin and von Willebrand factor) compared to VEGF-A alone. Ang II alone failed to induce EC marker expression. MSCs differentiated with the combination of Ang II and VEGF-A were capable of forming capillary tubes using an in vitro angiogenesis assay. Induction of EC marker expression was greatly attenuated by co-treatment of Ang II/VEGF-A with the AT2R antagonist PD123319, but not the AT1R antagonist telmisartan.

Conclusions
We report the presence of functional AT2R receptor on porcine bone marrow-derived MSCs, where it positively regulates EC differentiation. These findings have significant implications toward therapeutic approaches based on activation of AT2R, which could be a means to stimulate regeneration of damaged endothelium and prevent vascular thrombosis.
Gambling problems in bipolar disorder in the UK: Prevalence and
distribution
Authors: Jones Lisa , Metcalf Alice , Gordon-Smith Katherine , Forty Liz , Perry Amy , Lloyd Joanne , Geddes John R. , Goodwin Guy M. , Jones Ian , Craddock Nick , Rogers Robert D. 
Published: June 2015

BackgroundNorth American studies show bipolar disorder is associated with elevated
rates of problem gambling; however, little is known about rates in the
different presentations of bipolar illness.AimsTo determine the prevalence and distribution of problem gambling in
people with bipolar disorder in the UK.MethodThe Problem Gambling Severity Index was used to measure gambling problems
in 635 participants with bipolar disorder.ResultsModerate to severe gambling problems were four times higher in people
with bipolar disorder than in the general population, and were associated
with type 2 disorder (OR = 1.74, P = 0.036), history of
suicidal ideation or attempt (OR = 3.44, P = 0.02) and
rapid cycling (OR = 2.63, P = 0.008).ConclusionsApproximately 1 in 10 patients with bipolar disorder may be at moderate
to severe risk of problem gambling, possibly associated with suicidal
behaviour and a rapid cycling course. Elevated rates of gambling problems
in type 2 disorder highlight the probable significance of modest but
unstable mood disturbance in the development and maintenance of such
problems.
Drosophila's contribution to stem cell research
Authors: Singh Gyanesh 
Published: June 2015

The discovery ofDrosophilastem cells with striking similarities to mammalian stem cells has brought new hope for stem cell research. A recent development inDrosophilastem cell research is bringing wider opportunities for contemporary stem cell biologists. In this regard,Drosophilagerm cells are becoming a popular model of stem cell research. In several cases, genes that controlledDrosophilastem cells were later discovered to have functional homologs in mammalian stem cells. Like mammals,Drosophilagermline stem cells (GSCs) are controlled by both intrinsic as well as external signals. Inside theDrosophilatestes, germline and somatic stem cells form a cluster of cells (the hub). Hub cells depend on JAK-STAT signaling, and, in absence of this signal, they do not self-renew. InDrosophila, significant changes occur within the stem cell niche that contributes to a decline in stem cell number over time. In case of agingDrosophila, somatic niche cells show reduced DE-cadherin and unpaired (Upd) proteins. Unpaired proteins are known to directly decrease stem cell number within the niches, and, overexpression ofupdwithin niche cells restored GSCs in older males also . Stem cells in the midgut ofDrosophilaare also very promising. Reduced Notch signaling was found to increase the number of midgut progenitor cells. On the other hand, activation of the Notch pathway decreased proliferation of these cells. Further research in this area should lead to the discovery of additional factors that regulate stem and progenitor cells inDrosophila.
O MÉTODO HISTÓRIA DE VIDA: DESVENDANDO A SUBJETIVIDADE DO INDIVÍDUO NO ESTUDO DAS ORGANIZAÇÕES
Authors: Maccali Nicole , Minghini Luciano , Walger Carolina de Souza , Roglio Karina de Déa 
Published: June 2015

<p>As agendas de pesquisa em processo decisório sugerem investigações com foco nos aspectos subjetivos da tomada de decisão, como os valores e a experiência de vida do gestor. Sugerem, também, a adoção de novos métodos para explorar esse fenômeno, considerando que os métodos mais utilizados apresentam limitações no entendimento das subjetividades do indivíduo. Com base nessas agendas, no presente artigo, propõe-se a utilização do método história de vida como possibilidade metodológica para a investigação das influências dos<br />aspectos subjetivos no processo de decisão. Como forma de evidenciar as possibilidades do método, apresentase um caso ilustrativo. Neste sentido, a principal contribuição deste artigo é evidenciar a possibilidade de utilização do método história de vida no estudo dos aspectos subjetivos do processo de tomada de decisão do indivíduo.</p>
INCUBADORA TECNOLÓGICA DE CAXIAS DO SUL: INOVAÇÃO TECNOLÓGICA SOB A PERSPECTIVA DA HÉLICE TRÍPLICE
Authors: Tisott Priscila Bresolin , Nespolo Daniele , Dias Deise Taiana de Ávila , Olea Pelayo Munhoz , Milan Gabriel Sperandio 
Published: June 2015

<p>O objetivo deste estudo foi identificar como o conceito da Hélice Tríplice está aplicado nas atividades da Sociedade Incubadora Tecnológica de Caxias do Sul. Para atingir este objetivo, foram realizadas entrevistas semiestruturadas em profundidade com a ITEC, a Universidade de Caxias do Sul, Câmara da Indústria, Comércio e Serviços de Caxias do Sul, Prefeitura de Caxias do Sul e Comlink Equipamentos Eletrônicos, uma das empresas que obteve êxito na incubação.<br />A análise do conteúdo dos resultados mostrou que a atuação dos agentes se dá, principalmente, por meio de recursos financeiros. Já os principais benefícios obtidos pela Universidade com base nessa relação são o retorno do investimento realizado sob a forma de patentes formalizadas, conquista de novos alunos e reconhecimento como uma Universidade tecnológica, mpreendedora e inovadora. Os benefícios percebidos pelo setor industrial são a capacitação das empresas e empreendedores, enquanto o Poder Público Municipal percebe como benefícios a redução na mortalidade das empresas e a geração de retorno financeiro sob a forma de impostos. As conclusões mostram que o conceito da Hélice Tríplice, desenvolvido por Etzkowitz (2009), constitui uma forma de atuação cooperativa e participativa na Incubadora Tecnológica, ainda que mais segmentos da sociedade pudessem ser atendidos.</p>
ENSINO ORIENTADO PELO RESPEITO ÀS INTELIGÊNCIAS MÚLTIPLAS: AS CONTRIBUIÇÕES DE HOWARD GARDNER PARA O EXERCÍCIO DA DOCÊNCIA
Authors: Pavan Kelly Roman 
Published: June 2015

<p>A obra de Howard Gardner intitulada Inteligências múltiplas – A teoria na prática foi publicada no Brasil há quase vinte anos. Embora não se trate de obra recente, vale a pena revisitá-la com um olhar crítico e destacar suas importantes contribuições para o exercício da docência.</p>
Effects of [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O and [Cu(2-MeSNic)2(H2O)]2 on Candida albicans
Authors: Kaliňáková Barbora , Hudecová Daniela , Segľa Peter , Palicová Martina , Švorec Jozef 
Published: June 2015

Abstract
Probable mode of action of new copper complexes of 2-methylthionicotinate (2-MeSNic) of composition [Cu(2-MeSNic)2(H2O)2] and [Cu(2-MeSNic)2(MeNia)2(H2O)2]·H2O (where MeNia is N-methylnicotinamide) is described. Both partial growth inhibition of Candida albicans (IC50 ≥ 1.78 mmol·L−1, MIC ≥ 2.5 mmol·L−1) and leak of proteins into the extracellular space (more than 80 %) were observed in the presence of these copper complexes. The membrane damage was detected by staining with Hoechst 33342, propidium iodide and methylene blue. Ascorbic acid potentiated antifungal activity of copper complexes approximately seven-fold and induced the oxidative stress, respectively. The production of intracellular reactive oxygen species was visualized by dichlorofluorescein. Thiobarbituric acid-reactive substances were formed as a by-product of lipid peroxidation.
Impact of the pore solution on the aqueous diffusion through porous materials
Authors: Mercado-Mendoza Hugo , Lorente Sylvie 
Published: June 2015

AbstractThis paper reviews the main advances of our
group on the measurements of the diffusion coefficient
through partially saturated porous materials. The technique
that we developed is based on the definition of
an equivalent electrical model reproducing the behavior
of the porous microstructure. The electrical characteristics
are measured by means of Electrochemical Impedance
Spectroscopy. After validating the approach on saturated
samples, the method was applied to partially saturated
materials. We showed that the pore solution ionic composition
has not impact on the results, and documented the
effect of the pore size distribution on the macroscopic diffusion
coefficient.
Constructal vascularized structures
Authors: Cetkin Erdal 
Published: June 2015

AbstractSmart features such as self-healing and selfcooling require bathing the entire volume with a coolant or/and healing agent. Bathing the entire volume is an example of point to area (or volume) flows. Point to area flows cover all the distributing and collecting kinds of flows, i.e. inhaling and exhaling, mining, river deltas, energy distribution, distribution of products on the landscape and so on. The flow resistances of a point to area flow can be decreased by changing the design with the guidance of the constructal law, which is the law of the design evolution in time. In this paper, how the flow resistances (heat, fluid and stress) can be decreased by using the constructal law is shown with examples. First, the validity of two assumptions is surveyed: using temperature independent Hess-Murray rule and using constant diameter ducts where the duct discharges fluid along its edge. Then, point to area types of flows are explained by illustrating the results of two examples: fluid networks and heating an area. Last, how the structures should be vascularized for cooling and mechanical strength is documented. This paper shows that flow resistances can be decreased by morphing the shape freely without any restrictions or generic algorithms.
Enumeration search method for optimisation of stacking sequence of laminated composite plates subjected to buckling
Authors: Sedyono Joko , Hadavinia Homayoun , Venetsanos Demetrios , Marchant Denis R. 
Published: June 2015

AbstractEnumeration search method (ESM) checks all possible combinations of design variables in a bottom-up approach until it finds the global optimum solution for the design conditions. In this paper an optimum design of a multilayered laminated plate made of unidirectional fibre reinforced polymer (FRP) composite subject to uniaxial compression is sought.ESMtogether with classical laminated plate theory (CLPT) has been used to find the lightest laminate for maximizing the buckling load capable of providing structural stability for a set target uniaxial compression load. The choice of the design variables is limited to 4 possible fibres orientation angles (0,90,-45,+45) and the sequence of the laminate, making the problem an integer programming. Experimental and finite element analyses were used to verify the optimum solution. It has been shown that the exhaustive enumeration search method is a powerful tool for finding the global optimum design.
Female Sport Participation In South African Rural Schools: Analysis Of Socio-Cultural Constraints
Authors: Kubayi Ntwanano Alliance 
Published: June 2015

Abstract
This study was carried out to examine constraints to sport participation among female secondary school students in Hlanganani rural area, Limpopo Province, South Africa. A total of 101 female students aged 17–24 years from four secondary schools were recruited to participate in the study. A self-administered questionnaire was used to collect data. Results indicated that the dress code, lack of energy, lack of family support and family commitment were identified as major constraints to sport participation among female students. The results of this study provide practical implications for promoting and developing female sports programmes in rural schools. This study suggests that stakeholders such as parents, peers, and teachers should motivate and encourage female students to participate in school sport. Additionally, the study recommended that in order to promote sport participation in rural areas, the values, norms, beliefs, attitudes and customs that restrict females from participating in sport and physical activity should be dissented.
Organisational Communication In The Field Of Child Protection. Passing From Fatalism To Organisational Democracy In The Romanian Public Space
Authors: Petrică Ion , Tomiţa Mihaela 
Published: June 2015

Abstract
Considered a fundamental element of human existence, communication has been used since ancient times for the purpose of “transmitting something to the others also”. Although the term of “communication” has Latin origin, the first practical preoccupations for this issue are found in the Ancient Greeks’ works and were related to oratory which, in its turn, was promoted as “art of the word”. Even if in the literature the notion of “communication” was defined in a complex manner, being assigned numerous valences. Theoreticians found that, on the level of human communication, two common elements clearly appear for each of the meanings of this term. Thus, on the one hand, communication is perceived as a true process of information transmission, and on the other hand no human action, irrespective of the level of its realisation, can be conceived outside this process. Beyond the completion of the main theoretical models for the phenomena of organisational communication, the present article attempts to forward the author's personal diagnosis of a child protection institution, more precisely the General Division for Social Work/Assistance and Child Protection Caraş-Severin (DGASPC), valuing a wide-range methodology (analysis of social documents, interview, and structured observation). Thus, by combining the quantitative endeavour with the qualitative one, we highlighted the existence of a formal ascendant vertical communication, opposed or complementary to the descendant vertical one characterising most of Romanian organisational practices. The special institutional specificity of DGASPC turns this organisational territory into a possible model of interpersonal relationing, different from the general specificity existing in Romanian public institutions where the boss dictates.
Control of cockchafer Melolontha spp. grubs – a review of methods
Authors: Woreta Danuta 
Published: June 2015

Abstract
The paper presents a review of information on control measures used to prevent damages due to cockchafer Melolontha spp. grubs in European countries including Poland. There are described the methods used at times when mechanical and chemical treatments were applied as well as those under advanced research. In the past, cockchafer grubs were manually removed from plowed soils. Later on, chemical pest control commenced in plant protection practice, and a range of insecticides were either spotted directly onto plants or applied into planting rows, otherwise an entire planting area was treated. In that case, powder or granular insecticide formulations were mixed with upper soil layers, but liquid insecticides were poured into the soil around seedlings. The active substance of initial plant protection products used in Poland to control cockchafer grubs was lindane (organochlorine neurotoxin), which showed pretty high efficacy. Nevertheless, organochlorine products must have been withdrawn from the market and less damaging to the environment pesticides (organophosphates and carbamates) were recommended for use in forest protection against cockchafer grubs. As a result of progressive restrictions concerning application of chemicals into forest environment, alternate solutions have been sought, e.g. biological methods of cockchafer grub control have been tested. Up to date studies showed insecticidal properties of bacteria, fungi and nematodes. However, even though the knowledge and awareness of practitioners have raised with time, the problem of cockchafer grub populations damaging forest crops has not yet been solved and seems to be more and more difficult to overcome.
PÓŹNOGLACJALNE I HOLOCEŃSKIE OSADY WYBRANYCH TORFOWISK DOLINNYCH REGIONU ŁÓDZKIEGO W NAWIĄZANIU DO ZMIAN PALEOŚRODOWISKOWYCH
Authors: Forysiak Jacek , Kloss Marek , Obremska Milena , Żurek Sławomir 
Published: June 2015

AbstraktArtykuł przedstawia wyniki badań geomorfologicznych, geologicznych oraz paleobotanicznych, przeprowadzonych na czterech torfowiskach dolinnych regionu łódzkiego (Bęczkowice, Kopanicha, Mianów i Wilczków). Są to obiekty funkcjonujące w warunkach niewielkiego wpływu rzek na przebieg procesów torfotwórczych. Misy opisywanych torfowisk powstały w różnym czasie, od przełomu plenivistulianu i późnego vistulianu po początek holocenu. Trwałe zatorfienie również następowało w różnym czasie. Wszystkie z analizowanych torfowisk można określić jako torfowiska paludyfikacyjne. Udokumentowane osady biogeniczne pozwalają na rekonstrukcję zmian środowiska w wybranych torfowiskach w późnym vistulianie i holocenie.
SKŁAD CHEMICZNY OSADÓW BAGIENNYCH Z MARTWEJ DOLINY BALIN-CHROPY (STANOWISKO WILCZKÓW, PRADOLINA WARSZAWSKO-BERLIŃSKA)
Authors: Borówka Ryszard K. , Tomkowiak Julita , Okupny Daniel , Forysiak Jacek , Bieniek Bartosz 
Published: June 2015

AbstraktAnalizę geochemiczną przeprowadzono dla osadów organicznych z torfowiska Wilczków w południowej części pradoliny warszawsko-berlińskiej. Z profilu Wil-1, zlokalizowanego w środkowej części martwej doliny Balin-Chropy, pobrano do analiz geochemicznych 97 próbek osadów (do głębokości 4,94 m), reprezentujących 1-cm segmenty rdzenia. W próbkach tych oznaczono straty na prażeniu w temperaturze 550°C, informujące o zawartości materii organicznej oraz oznaczono pierwiastki o wymowie paleogeograficznej: Na, K, Mg, Ca, Fe, Mn i metale ciężkie: Cu, Zn oraz zawartość węgla ogólnego, azotu ogólnego i siarki ogólnej. Stosując analizę skupień wydzielono osiem lokalnych poziomów geochemicznych (LGZ), różniących się istotnie pod względem składu chemicznego osadów. Dla serii osadów torfowych określono również związki korelacyjne pomiędzy analizowanymi metalami, a także materią organiczną oraz obliczonymi wskaźnikami geochemicznymi: Ca/Mg, Na/K, Na+K+Mg/Ca, Fe/Ca, Fe/Mn, Cu/Zn, pozwalających na rekonstrukcję warunków akumulacji analizowanych osadów. W toku analizy składowych głównych zidentyfikowano 12 głównych czynników, z których wybrano trzy pierwsze – PC1, PC2 i PC3, wyjaśniające łącznie 84,7% wariancji. Do czynników kształtujących skład chemiczny holoceńskich osadów organicznych w południowej części pradoliny warszawsko-berlińskiej zaliczono produktywność biologiczną ekosystemu torfowego, procesy denudacji chemicznej i mechanicznej w zlewni, warunki oksydacyjno-redukcyjne oraz zmiany poziomu wody na torfowisku.
SKŁAD CHEMICZNY OSADÓW BAGIENNYCH Z DOLINY NERU (TORFOWISKO MIANÓW, WYSOCZYZNA ŁASKA)
Authors: Borówka Ryszard K. , Tomkowiak Julita , Okupny Daniel , Forysiak Jacek 
Published: June 2015

AbstraktAnalizę geochemiczną przeprowadzono dla osadów organicznych z torfowiska Mianów w środkowym odcinku doliny Neru. Z profilu Mi-1, zlokalizowanego we wschodniej części mokradła, pobrano do analiz geochemicznych 40 próbek osadów (do głębokości 1,97 m), reprezentujących 2,5-cm segmenty rdzenia. W próbkach tych oznaczono straty na prażeniu w temperaturze 550°C, informujące o zawartości materii organicznej oraz oznaczono pierwiastki o wymowie paleogeograficznej: Na, K, Mg, Ca, Fe, Mn i metale ciężkie: Cu, Zn i Pb. Stosując analizę skupień wydzielono pięć lokalnych poziomów geochemicznych (LGZ), różniących się istotnie pod względem składu chemicznego osadów. Dla serii osadów torfowych określono również związki korelacyjne pomiędzy analizowanymi metalami, a także materią organiczną oraz obliczonymi wskaźnikami geochemicznymi: Ca/Mg, Na/K, Na+K+Mg/Ca, Fe/Ca, Fe/Mn, Cu/Zn, pozwalających na rekonstrukcję warunków akumulacji analizowanych osadów. W toku analizy składowych głównych zidentyfikowano 10 głównych czynników, z których wybrano trzy pierwsze – PC1, PC2 i PC3, wyjaśniające łącznie 86,53% wariancji. Do czynników kształtujących skład chemiczny holoceńskich osadów organicznych w dolinie Neru zaliczono warunki oksydacyjno-redukcyjne, sorpcję metali przez autochtoniczną materię organiczną, względne zmiany procesów denudacji mechanicznej w zlewni mokradła oraz właściwości akumulacyjne roślin w stosunku do określonych pierwiastków.
Analysis on the dynamic deformations of the images from digital film sequences
Authors: Markowski Tomasz 
Published: June 2015

Abstract
Image sequences, in particular digital video sequences, are characterised by the features which result in their high potential as measurement data. However, as early as at the stage of visual assessment of digital film images, originating, in particular, from amateur cameras, occurrence of some deformations may be observed, which may highly influence the results of measurements performed using these images; such deformations differ from deformations occurred in the case of static photographic images. It results both, by the method of image recording, using an electronic shutter and interlaced or progressive scanning, as well as the method of file recording and compression. It is worth to notice the systematic nature of such deformations, which highly depend on mutual motions of a camera and recorded objects. The objective of presented research works was to develop the mathematical description of image deformations, as a function of motion parameters. This would allow for adaptation of the camera calibration process to the demands of sequential imaging, as well as for modification of algorithms of measurements using self-calibration, and, as a result, minimisation of deformations. Another objective was to analyse the influence of deformations, typical for digital film images, on the results of measurements performed using these images, by means of series of experiments, which were based on multiple calibration of static and a moving camera, also with the use of a spatial test field. The first part was made by developing formulas based on some geometric relations, using some simplifications. On the stage of experimental research a certain degree of compatibility of experimental results and theoretical assumptions were confirmed.