bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diaper Dermatitis with Granuloma Following Surgery for Hirschsprung's Disease
Authors: Tamaki Kunihiko , Yokomori Kinji 
Published: April 2015

AbstractThree Japanese infants who developed diaper dermatitis with granuloma on the perianal area following surgery for Hirschsprung's disease were reported. These granulomas are more similar to those seen in diaper area granuloma of the aged rather than to those of granuloma gluteale infantum.
Drug Eruption Caused by Nitrazepam in a Patient with Severe Pustular Psoriasis Successfully Treated with Methotrexate and Etretinate
Authors: Shoji Akinobu , Kitajima Jun'ichi , Hamada Toshio 
Published: April 2015

AbstractAn allergic drug eruption due to nitrazepam was reported in a patient who had been administered methotrexate and etretinate as concurrent therapies for severe pustular psoriasis. The clinical manifestation included a moon face‐like appearance with edema, a diffuse erythematous rash over almost the entire body the day after taking nitrazepam and, two days later, eroded lesions developing on the cheeks, axillae and genitocrural regions. It was proved by provocation that this drug eruption had an allergic nature.
Histamine Release by Scraching Inflamed Skin
Authors: Nishioka Kiyoshi , Katayama Ichiro , Doi Toshiaki 
Published: April 2015

AbstractScratching is an exacerbating factor of skin diseases. Plasma histamine level was assessed before and after scratching with a scratch device. Inflamed skin of patients with atopic dermatitis released slight but significant amounts of histamine by scratching, but healthy skin was not affected by scratching.
Biochemical Study of Fecal Porphyrin in Porphyria Cutanea Tarda
Authors: Nonaka Shigeo , Ohgami Taro , Murayama Fumio , Yamashita Kazunori , Nagato Nori , Watanabe Masahisa , Irifune Hiroko , Yoshida Hikotaro 
Published: April 2015

AbstractFecal, urinary and erythrocyte porphyrin analyses using the solvent extraction method were performed in 26 patients with porphyria cutanea tarda (PCT) and 144 normal controls. The levels of fecal uroporphyrin (UP) and coproporphyrin (CP) were markedly increased in the PCT group, especially in comparison with the protoporphyrin (PP) level. The values of the UP/PP and CP/PP ratios in the feces were also elevated over those in the control group. It appears that fecal porphyrin excretion is basically similar to urinary porphyrin excretion in PCT. The analysis of fecal porphyrins and the observation of fecal UP/PP and CP/PP ratios may be helpful in the biochemical diagnosis of PCT. In particular, the elevation of CP/PP ratio is characteristic of PCT.
Effect of Human Fibroblast Interferon on Normal Human Monocyte Activation Induced by a Factor Found in Sarcoidosis Sera
Authors: Baba Toru , Yamaguchi Koji , Matsushima Yuji , Hoshino Minoru , Ochiya Takahiro , Baba Akiko , Uyeno Kenichi 
Published: April 2015

AbstractEffect of human fibroblast interferon (IFN‐β) on normal human monocyte activation induced by the monocyte activating factor found in sarcoidosis sera was studied. Spreading was used as one indicator of monocyte activation. IFN‐β was shown to inhibit spreading of normal human monocytes induced by the activating factor. The inhibitory activity of IFN‐β against monocyte spreading was adsorbed with monoclonal antibody to human IFN‐β‐Sepharose beads. Increases in phagocytosis and glucose consumption of normal human monocytes induced by the activating factor was also inhibited by IFN‐β. Recombinant IFN‐β also showed similar inhibitions.
Electron Microscopic Detection of p97 Antigen on Cell Membranes of Human Melanoma Cell Lines
Authors: Uno Akihiko , Hori Yoshiaki , Saida Toshiaki 
Published: April 2015

AbstractWe investigated the ultrastructural distribution pattern of P97 antigen on the cell surface of cultured human melanoma cells with ferritin‐labelled antibody and confirmed the sialic acid nature of the epitope by use of neuraminidase and wheat germ agglutinin, a lectin which binds specifically to sialic acid.At the ultrastructural level, the melanoma‐associated antigen, p97, exists mainly as a solitary element on the cell surface of the cultured human melanoma cell. In small areas, however, the antigen assembles to form small clusters. Our ultrastructural study supports the thesis that the epitope of p97a consists of sialic acid.
Negative Immunolabelling for Factor VIII‐related Antigen in the So‐called “Sclerosing Haemangiomas” (Histiocytofibromas) of the Skin
Authors: Kanitakis Jean , Hermier Claude , Mauduit Gilles , Thivolet Jean 
Published: April 2015

AbstractThe cellular composition of the so‐called “sclerosing haemangioma” (or histiocytofibroma‐HF) of the skin has long been controversial and is still uncertain. Histiocytes, fibroblasts and endothelial cells have been considered as the cell‐type of origin of these lesions. In order to assess the alleged endothelial derivation of HF cells, we performed an immunohistochemical study of 22 cases of HF of the skin using a polyclonal antibody to factor VIII‐related antigen (F VIII RA) and an avidin‐biotin‐peroxidase technique on formalin‐fixed, paraffin‐embedded sections. F VIII RA distinctly decorated the small vessels around and inside the tumours, but HF cells themselves were negative. This result, which agrees with previous enzyme‐histochemical studies, speaks against the endothelial origin of HF cells.
Recovery of Skin Barrier Function in Psoriasis Following Standardized Trauma
Authors: den Arend J.A.C.J. , van de Kerkhof Peter C.M. 
Published: April 2015

AbstractFollowing a standardized trauma to the clinically uninvolved skin of psoriatic patients, an overresponse of several metabolic processes occurs. In the present study, barrier functions of normal looking skin of psoriatic patients and healthy controls were assessed during 5 weeks following a standardized injury. Surprisingly, recovery rates of both groups proved to be identical. The significance of this finding in the epidermal homeostasis of psoriasis is discussed.
Effects of PSK Ointment and OK‐432 Ointment of Pseudomonas Burn Wound Infection in Mice
Authors: Watanabe Takashi , Saito Hajime 
Published: April 2015

AbstractWhen Pseudomonas aeruginosa was inoculated onto wound surfaces of burned mice, PSK ointment consisting of Solbase and PSK (a protein‐bound polysaccharide from Coriolus versicolor) and OK‐432 ointment consisting of Solbase and OK‐432 (an attenuated Streptococcus pyogenes Su) enhanced the clearance of Pseudomonas aeruginosa, and promoted the healing of the burn wounds. Bacteria‐eliminating and wound‐healing effects of these ointments were much the same or somewhat better than those of commercially available ointments such as Geben (Silvadene) and Mafatate (Sulfamylon) creams.
Three Cases of Sporotrichosis with Numerous Fungal Elements
Authors: Mohri Shinobu , Nakajima Hiroshi , Kurosawa Tsutae , Takanashi Yuzo , Takahashi Yasuhide , Nagai Ryukichi 
Published: April 2015

AbstractThree cases of sporotrichosis with numerous fungal elements were reported. Clinically these cases showed nodules to plaques with ulceration. Histologically, they showed unusual features, including histiocytic granuloma in the upper to middle dermis which contained few neutrophils but were surrounded by lymphocytes and epithelioid cells. There was no formation of an abscess. Fungal elements were found in abundance in the histiocytic granuloma, mainly in the form of oval or round spores of varying sizes, some of them produced germ tubes. No asteroid bodies were found. The spores stained positively with HE and PAS, and, in case 1, with anti‐Sporothrix schenckii antiserum. All three cases had been treated with topical corticosteroid ointment. In one case, after the topical corticosteroid treatment was stopped, the histological pattern returned to the usual features seen in sporotrichosis, and fungal elements decreased significantly. This unusual histological pattern was also observed in a specimen from a healthy control infected with Sporothrix schenckii and treated with steroid ointment. These histological and fungal patterns may be possibly induced by the local immunodeficiency following topical steroid treatment.
Evaluation of the Skin Test Reactions in Patients with Delayed Type Rash Induced by Penicillins and Cephalosporins
Authors: Aihara Michiko , Ikezawa Zenro 
Published: April 2015

AbstractSkin test reactions were evaluated in 39 patients with delayed type generalized drug eruptions in which penicillins (PCs) or cephalosporins (CSs) were proved to be the causative drugs by intradermal testing and provocation. They were also compared with the experimental PC/CS rash of guinea pigs reported previously. The skin test was positive in 37 (95%) of 39 patients tested and in 13 (87%) of 15 patients, in whom the causative drugs were identified by provocation, in which intradermal testing with 2 to 10% PC/CS in saline solution was employed and the skin test reaction was observed not only 24 hours later but also 6 to 9 hours later. It is considered that the skin test is a valuable in vivo test for the diagnosis of drug allergy. From the skin test reaction, it appears that the PC/CS rash is classified into at least two different reactions, the Jones‐Mote type reaction and the tuberculin type reaction. No cross reactions between PC and CS derivatives were observed in either the skin testing or the provocation in man, which is the same pattern seen in the animal model. Cross reactions among the PC derivatives in man were not found as frequently as expected from the results in animals.
A New Keratinolytic Proteinase from Clinical Isolates of Trichophyton mentagrophytes
Authors: Tsuboi Ryoji , Ko Ik‐Jun , Matsuda Kazuko , Ogawa Hideoki 
Published: April 2015

AbstractA new keratinolytic proteinase produced by each of three clinical isolates of Trichophyton mentagrophytes was found in culture filtrates containing human strarum corneum as a nitrogen source. The partially purified proteinase fraction exhibited serine proteinase‐like characteristics in spite of its weakly acidic optimum pH. The existence of a keratinolytic proteinase with enzyme activity under acidic conditions could be an important pathogenic element in dermatophytic invasion of weakly acidic healthy skin.
Amyloidogenesis in a Case of Cutaneous Amyloidosis: An Electron Microscopic Study Using the Refixation‐reembedding Method
Authors: Horiguchi Yuji , Ikai Kouichi , Danno Kiichiro , Horiguchi Michiko , Imamura Sadao 
Published: April 2015

AbstractA case of the hyperkeratotic type of macular amyloidosis was electron microscopically examined using the refixation‐reembedding method. All the cytoid bodies seen in the dermis were amyloid islets associated with malformed basal lamina‐like material. The uppermost amyloid islets were in close apposition to the basal cells, which had neither hemidesmosomes nor intact basal lamina. Anchoring fibril‐like filaments were seen between the amyloid islets and the basal lamina‐like material. Many fibrillar bodies and clusters were present in the spinous layer, but not in the dermis. Masses of eosinophilic, PAS‐positive ovoid bodies seen in the horny layer were tightly packed fibrillar bodies shifted from the spinous layer to the horny layer. These findings suggest that the disturbed basal cells produce an amyloid substance in the dermis, as well as malformed basal lamina. The colloid bodies formed by the degeneration of the basal cells seemed to shift up to the horny layer but not to contribute to the amyloidogenesis in this case.
Mechanism of Blister Formation in Porphyria Cutanea Tarda: I. Histopathological Observation of Blisters in Three Cases of Porphyria Cutanea Tarda
Authors: Nagato Nori , Nonaka Shigeo , Ohgami Taro , Murayama Fumio , Yamashita Kazunori , Irifune Hiroko , Watanabe Masahisa , Yoshida Hikotaro 
Published: April 2015

AbstractWe performed a histopathological investigation of the bullous lesions in 3 cases of porphyria cutanea tarda. All cases showed subepidermal bullae by light microscopy. PAS positive materials were present on the roof of the bullae and partially present on their bases. Electron‐microscopically, the basal lamina was clearly recognized on the base. From these results, we suggest that the blister in porphyria cutanea tarda occurs initially within the junctional zone; this initial bulla may quickly change into a dermolytic bulla with additional stimulation.
Contemplative Immune Mechanism of Lucio Phenomenon and Its Global Status
Authors: Sehgal Virendra N. , Srivastava Govind , Sharma Vinay K. 
Published: April 2015

AbstractLucio's phenomenon is infrequent in our experience. Only 2 young men on multi‐drug therapy were seen among 36 erythema nodosum leprosum reaction cases in the last four years. It first appeared as extensive crops of puerpuric macules which transformed into dome‐shaped polygonal pustules and multiangular, bizarre, punched out ulcers. Necrotic greyish‐white debris was prominent the histological features were diagnostic. Multi‐drug therapy seemed to have instigated the process. The classical as well as the alterative pathway of complement activation is incriminated in its probable immune mechanism.
Cutaneous Changes in Trichinellosis Seen in Japan
Authors: Hanada Katsumi , Hashimoto Isao , Yamaguchi Tomio , Ezaki Yoshihiko 
Published: April 2015

AbstractSince 1974, three outbreaks of human trichinellosis have been reported from Japan with a total of 87 cases. A characteristic skin rash was seen together with muscle pain, fever, malaise, periorbital edema and marked eosinophilia. The rash appeared in all cases as fine red maculopapular plaques with an urticarial character. Although the reason for the high frequency of the rash is obscure, it seemed to be one of the most valuable symptoms of human trichinellosis in Japan.
Treatment of Severe Psoriasis with Low Dose Cyclosporin A and the Effect on the Helper‐suppressor T Cell Ratio in Peripheral Blood
Authors: Sasaki Tetsuo , Ikezawa Zenro , Nakajima Hiroshi 
Published: April 2015

AbstractOral administration of cyclosporin A (CsA) 14 mg/kg/day has been reported to improve psoriasis in a double‐blind study. The purpose of this study was to evaluate the efficacy of a lower dose of CsA in severe psoriasis and to monitor lymphocyte subpopulations in peripheral blood in order to detect its mechanism of action. Eleven patients with severe, active psoriasis were treated only with oral administration of 5 mg/kg/day CsA twice a day for 3 months, during which they were closely examined, including single‐ and two‐color analysis of lymphocyte surface markers by flow cytometry using monoclonal antibodies. Seven patients showed improvement within a week, and the others within 2‐3 weeks. Five patients had total remission, 2 showed marked improvement, and 4 showed moderate improvement; no clinically important side effects, except hypertrichosis in 2 females were seen. In the peripheral blood of patients treated with CsA there was a decrease in the percentages of helper inducer (CD4+4B4+)T(Thi) cells, while no significant decrease was found in those of suppressor inducer (CD4+2H4+)T(Tsi), suppressor effector (CD8+CD11+)T(Tse), or cytotoxic (CD8+CD11‐)T(Tc) cells, resulting in a decrease in the ratio of Thi to Tsi, Tse and Tc. The activated helper (CD4+HLA‐DR+)—suppressor (CD8+HLA‐DR+)T cell ratio was also decreased. These immunological findings obtained from the patients were consistent with in vitro studies of CsA reported earlier and may well explain the effectiveness of CsA in psoriasis as observed in this study.
Monocyte Activating Factor Originally Found in Sarcoidosis Sera
Authors: Baba Toru , Matsushima Yuji , Onozaki Kikuo , Baba Akiko , Uyeno Kenichi 
Published: April 2015

AbstractThe culture medium for normal human monocytes pretreated with interleukin (IL)‐1, either α and β, induced normal human monocytes to spread and to increase Fc (IgG) receptor sites, angiotensin converting enzyme production and glucose consumption of normal human monocytes, suggesting that this medium contains a monocyte activating factor(s). On TSK G3000SW gel filtration, the major monocyte activating factor in the culture medium and that in sarcoidosis serum were eluted at the same position (with a molecular weight of about 70,000). Thus, IL‐1 may be able to induce normal human monocytes to produce a monocyte activating factor quite similar to that found in sarcoidosis serum.
Monocyte Activating Factor Originally Found in Sarcoidosis Sera
Authors: Baba Toru , Matsushima Yuji , Baba Akiko , Hanada Takashi , Uyeno Kenichi 
Published: April 2015

AbstractThe culture medium of a human acute monocytic leukemia cell line (THP‐1) was able to induce normal human monocytes to spread. No such ability was found either in the culture medium of a promyelocytic leukemia cell line (HL‐60) nor in that of a diploid human fibroblast cell line (Flow 7000). Gel filtration of the culture medium of THP‐1 cells on a size exclusion column (TSKgel G3000SW) revealed that the most obvious monocyte spreading activity was found in the fraction eluted at the position with a molecular weight of about 70,000. This fraction was also able to increase production of angiotensin converting enzyme and Fc receptor sites for IgG on normal human monocytes.
Phrynoderma: A Review of 105 Cases
Authors: Nakjang Yaovares , Yuttanavivat Tipaporn 
Published: April 2015

AbstractA study of 105 patients with phrynoderma revealed the usual clinical feature—follicular keratotic papules at the elbows and knees. In severe cases, the papules might extend to the forearm, thigh, chest, back and abdomen; the face, hands, feet, and genito‐anal area were not involved. Only 3% of cases had serum vitamin A levels lower than normal. The most common histologic findings are normo‐ or hypergranulosis and retained parakerotic cells within the follicular plug. These histologic features differentiate phrynoderma from keratosis pilaris.
Reiters' Disease: A Radiological Assessment of Joints
Authors: Sehgal Virendra N. , Jain Manoj K. , Srivastava Govind , Saha Madan M. 
Published: April 2015

AbstractOne married and one unmarried man with Reiters' disease are reported. The disease is characterized by well‐recognized cutaneous lesions and conjunctivitis. However, affliction of the joints of the lower and upper extremities was striking. The conspicuous Roentgenographic features included extensive juxta‐articular rarefaction, moderate to severe narrowing of joint spaces, subluxation/dislocation of small joints of the hands and feet, and fixed flexion deformity with ankylosis of the knees. Mutilating arthritis (Launois's deformity) of the feet was also contributory.
Spontaneous Photo‐onycholysis
Authors: Horio Takeshi 
Published: April 2015

AbstractA 48‐year‐old house wife developed photo‐onycholysis in all her fingernails. It was not associated with any cutaneous eruption or photosensitivity on other parts of the skin. The patient was not taking any drugs. She has been followed up by the present author for the last 10 years. Every year, onycholysis has begun in May, worsened in July and August, and resolved spontaneously by the beginning of November. These observations strongly suggest the role of sunlight and exclude involvement of any photosensitizing drug in this disorder.
The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene
Authors: Bang Dongsik , Nakagawa Shojiro , Oka Daisuke , Takei Yoji , Jinno Yoko , Ueki Hiroaki 
Published: April 2015

AbstractThe distribution of 2,4‐dinitrophenyl (DNP) groups on Thy‐1 positive epidermal cells in vivo after 2,4‐dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin‐separated mouse ear skin painted with DNCB were double‐stained for DNP groups and Thy‐1 protein using FITC‐anti‐DNP antibody, biotinylated anti‐mouse Thy‐1,2 monoclonal antibody, and phyco‐erythrin conjugated avidin. There were on the average 1.7% Thy‐1+ ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti‐DNP followed by anti‐Thy‐1,2 incubation. The incidence of Thy‐1,2+ EC with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to the mode of DNCB Thy‐1+ EC binding.
Immunochemical Analyses of Prolidase Deficiency Sera
Authors: Yonemasu Kunio , Lapière Charles M. , Sasaki Takako , Nusgens Betty , Endo Fumio , Matsuda Ichiro , Ogata Akinori , Nagaki Kazuyoshi 
Published: April 2015

AbstractSera of a 30‐year‐old Italian female and a 32‐year‐old Japanese female with prolidase deficiency were analysed by two‐dimensional immunoelectrophoresis, rocket immunoelectrophoresis, and single radial immunodiffusion (SRID). In both patients, amounts of transferrin, albumin and IgM were significantly low, while concentrations of α1‐acid glycoprotein, α1‐antitrypsin, α1‐antichymotrypsin, ceruloplasmin, IgA and Clq were elevated in comparison with levels of these proteins in sera of healthy Italian and Japanese female adults (as normal controls). The amount of haptoglobin was conspicuously high in the Italian patient but remarkably low in the Japanese patient.
Diacylglycerol Mimics Phorbol Ester Induction of Morphological Changes in Low Ca2+ Regulated Epidermal Cells
Authors: Inohara Shinichi , Tateishi Haruyo , Sagami Seichiro 
Published: April 2015

AbstractIt is known that tumor‐promoting phorbol ester, 12‐O‐tetradecanoylphorbol‐13‐acetate, induces morphological changes in mouse epidermal cells cultured in low Ca2+ medium. In the present study, we found on phase‐contrast microscopic examination that synthetic diacylglycerol, l‐oleoyl‐2‐acetylglycerol, induced similar morphological features. Ultrastructural examination revealed that both materials used for treatment induced morphological changes, such as cell‐to‐cell contact. These two materials are known to directly activate protein kinase C in intact cells. On the other hand, cyclic AMP‐dependent protein kinase activating agents induced no morphological changes in the same culture system. Data obtained in this experiment suggest that the morphological changes brought about by 1‐oleoyl‐2‐acetylglycerol in this report may occur via the action of diacylglycerol on the protein kinase C system.
Lympho‐epidermal Interactions in Patients Receiving Human Langerhans cell Free Cultured Epidermal Allografts as a Skin Substitute
Authors: Demidem Aicha , Faure Michel , Mauduit Gilles , Rimokh Ruth , Thivolet Jean 
Published: April 2015

AbstractHuman Langerhans cell free epithelia can be cultured in vitro, and then can be used as epidermal allografts (EAG) without evidence of rejection. We studied the cellular basis of this phenomenon with mixed epidermal cell lymphocyte reactions (MELR). The capacity of donor‐derived epidermal cells to stimulate allogeneic control or recipient cells was abolished when stimulatory cells were Langerhans cell‐free cultured keratinocytes and respondors were obtained prior to grafting. Donor‐type cultured keratinocytes were able to induce a low response by host‐derived cells in assays conducted 2, 4 and 6 weeks after grafting, but not thereafter. They were unable to stimulate allogeneic cells unrelated to the recipient. The ability of crude epidermal suspensions with 2–4% Langerhans cells to stimulate host‐derived cells did not increase with time after grafting. No secondary type MELR could be evidenced, suggesting that grafting of EAG did not induce an in vivo immunization to class I antigens expressed by cultured epidermal cells. Lastly, host‐derived Langerhans cells were not able to restore the allostimulatory ability of Langerhans cell free epidermal cells from EAG donors when tested against host‐derived cells. This suggests that host‐derived Langerhans cells which colonize the grafts during the first few weeks following grafting cannot act in the presentation of foreign keratinocyte‐bound antigens, which may account for the absence of rejection noted.