bluuebunny/crossref_metadata_2025_split huggingface dataset:
Medication Management in Belgian Residential Care Facilities for Individuals With Intellectual Disability: An Observational Study
Authors: Joos Elke , Mehuys Els , Van Bocxlaer Jan , Remon Jean Paul , Van Winckel Myriam , Boussery Koen 
Published: March 2014

AbstractOrganizational aspects of the medication management process (MMP) have been investigated in hospital and nursing home settings. However, the amount of literature on medication management in residential care facilities (RCF) for individuals with ID is limited to two studies from the Netherlands, focusing only on drug administration errors. To assess the organization of the MMP in Belgian RCFs for people with ID, the authors undertook a cross‐sectional observational study in 34 Belgian RCFs for people with ID. Structured interviews were performed using a questionnaire: the first part (for the RCF directors) addressed administrative and policy issues. The second (for unit employees) and third parts (for medical office employees) addressed practical aspects of the MMP. The fourth part (for physicians) focused on the physician's role in the MMP, on the therapeutic drug formulary, and on communication with staff and delivering pharmacist. Standard operating procedures concerning the MMP were not available in 32% of the RCFs. About one‐quarter of the RCFs did not have a medication error reporting system, and most RCFs did not systematically (i.e., at least annually) review their MMP for failures. Only two participating RCFs were equipped with an electronic prescribing system. The role of the pharmacist was mainly limited to delivery of medication. Medication was mostly administered by nonmedically qualified staff (e.g., educators). Most frequently cited problems by the interviewees were problems in the medication preparation and administration stage. Most frequently cited possible improvement actions were education/sensitization of staff and improved communication among all stakeholders. The authors note that as this study revealed operational inadequacies, problem areas can be identified and targeted quality improvement actions can be undertaken.
Attention Deficit Hyperactivity Disorders Symptomatology Among Individuals With Down Syndrome
Authors: Edvardson Simon , Msallam Nadira , Hertz Pnina , Malkiel Sarah , Wexler Isaiah D. , Tenenbaum Ariel 
Published: March 2014

AbstractThe prevalence of attention deficit hyperactivity disorders (ADHD) among individuals with Down syndrome (DS) has been difficult to ascertain. This is because many of the phenotypic features of DS may resemble the clinical manifestations of DS and the myriad methodological challenges in diagnosing ADHD in children with intellectual disabilities (ID). With this in mind, the authors set to determine the prevalence of ADHD symptomatology in a cohort of children and young adults in a DS clinic. Subjects were 83 children and adults, ages 5–38, who were attending the Down Syndrome Center at the Hadassah Mount Scopus University Medical Center. All were screened for ADHD. Screening methods included review of medical files, telephone interviews, and use of the ADHD module of the Autism‐Tics, Attention‐Deficit/Hyperactivity disorder, and other Comorbidities questionnaire. It was found that 26 (31.3%) of the subjects fulfilled the Diagnostic and Statistical Manual of Mental Disorders (4th ed., text rev.) criteria for ADHD. Of these, 17 (65.4%) were diagnosed as predominantly inattentive type. Four (15.4%) were predominantly impulsive‐hyperactive, and five (19.2%) had the combined type. No statistically significant association was found between ADHD and age, sex, number of siblings, presence of heart disease, thyroid dysfunction, sleep disorders, or a family history of ADHD. The prevalence of ADHD symptomatology among individuals with DS in this representative sample is high compared with the general population. ADHD may be underdiagnosed in this special population, hence the need for systematic screening.
Plant phylogeography in arid Northwest China: Retrospectives and perspectives
Authors: MENG Hong‐Hu , GAO Xiao‐Yang , HUANG Jian‐Feng , ZHANG Ming‐Li 
Published: March 2014

AbstractDespite the absence of major Quaternary glaciations in arid Northwest China, significant climatic oscillations definitely impacted the evolution of the biota in situ. Phylogeography has grown as a discipline because it has provided explicit tools for the study of geographical subdivision among populations. But phylogeographical application for arid Northwest China has begun to blossom, which has provided evidence that aridification played a significant role in the increase of genetic diversity and species diversification. The time frame corresponds with Pleistocene climatic oscillations, which caused extreme aridity and the expansion of sandy deserts. In the Asian desert flora subkingdom and Eurasian forest subkingdom of Northwest China, the recurrent phylogeographical scenarios, identified by different case studies, broadly agree with longstanding biogeographic, floristic, and topographic concepts: (i) aridification promoted diversification and speciation of desert plants; (ii) desert expansion caused habitat fragmentation; (iii) the Altay–Tianshan Mountains included glacial refugia for plants; (iv) population expansion and recolonization from glacial refugia occurred during the postglacial period; and (v) desert plants persistence and alpine plants retreat during climate oscillations. We discuss the main phylogeographical findings in light of molecular and paleo‐environmental evidence, emphasizing notable gaps in our knowledge and outlining future research perspectives for disentangling the evolutionary history of this arid region's flora.
Acute Effects of Ivabradine on Dynamic Obstruction of the Left Ventricular Outflow Tract in Cats with Preclinical Hypertrophic Cardiomyopathy
Authors: Blass K.A. , Schober K.E. , Li X. , Scansen B.A. , Bonagura J.D. 
Published: March 2014

BackgroundIvabradine is a negative chronotropic drug with minimal effects on central hemodynamics. Its effect on dynamic obstruction of the left ventricular outflow tract (LVOT) in cats with hypertrophic cardiomyopathy (HCM) remains unknown.Hypothesis/ObjectivesIvabradine reduces dynamic obstruction of the LVOT in cats with HCM.AnimalsTwenty‐eight client‐owned cats with preclinical HCM and dynamic LVOT obstruction.MethodsRandomized, double‐blind, active‐control single dose study. Cats received a single dose of either ivabradine (0.3 mg/kg PO) or atenolol (2 mg/kg PO). Heart rate, echocardiographic variables, and systolic blood pressure (SBP) were recorded before and 3 hours after drug administration. Statistical comparisons were made using ANCOVA.ResultsPeak velocity in the LVOT was significantly decreased compared to baseline for both drugs; however, the effect was more prominent with atenolol (mean reduction 2.53 m/s; 95% CI 2.07–3.13 m/s) compared to ivabradine (mean reduction 0.32 m/s; 95% CI −0.04 to 0.71 m/s; P < .0001). Echocardiographic indices of systolic function were largely unchanged by ivabradine, but significantly reduced by atenolol.Conclusions and Clinical ImportanceA single dose of ivabradine decreases dynamic LVOT obstruction in cats with HCM, but the clinical effect is negligible and inferior compared to that achieved by atenolol.
Vaccine‐Associated Leptospira Antibodies in Client‐Owned Dogs
Authors: Martin L.E.R. , Wiggans K.T. , Jablonski Wennogle S.A. , Curtis K. , Chandrashekar R. , Lappin M.R. 
Published: March 2014

BackgroundLong‐term microscopic agglutination test (MAT) results after vaccination with 4‐serovar Leptospira vaccines are not available for all vaccines used in client‐owned dogs.Hypothesis/ObjectivesTo determine antibody responses of client‐owned dogs given 1 of 4 commercially available Leptospira vaccines.AnimalsHealthy client‐owned dogs (n = 32) with no history of Leptospira vaccination for at least the previous year.MethodsDogs were given 1 of 4 Leptospira vaccines on week 0 and then approximately on week 3 and week 52. Sera were collected before vaccine administration on week 0 and then within 3 days of week 3, within 2 days of week 4, and approximately on weeks 7, 15, 29, 52, and 56. Antibody titers against Leptospira serovars bratislava, canicola, grippotyphosa, hardjo, icterohemorrhagiae, and pomona and were determined by MAT.ResultsWhen compared among vaccines, MAT results varied in maximal titers, the serovars inducing maximal titers, and the time required to reach maximal titers. Each vaccine induced at least some MAT titers ≥1 : 800. Most dogs were negative for antibodies against all serovars 1 year after vaccination, and anamnestic responses were variable.Conclusions and Clinical ImportanceDogs vaccinated with Leptospira vaccines have variable MAT titers over time, and antibodies should not be used to predict resistance to Leptospira infection. MAT titers ≥1 : 800 can develop after Leptospira spp. vaccination, which can complicate the clinical diagnosis of leptospirosis.
Structural and molecular insights into novel surface‐exposed mucus adhesins from Lactobacillus reuteri human strains
Authors: Etzold Sabrina , MacKenzie Donald A. , Jeffers Faye , Walshaw John , Roos Stefan , Hemmings Andrew M. , Juge Nathalie 
Published: March 2014

SummaryThe mucus layer covering the gastrointestinal tract is the first point of contact of the intestinal microbiota with the host. Cell surface macromolecules are critical for adherence of commensal bacteria to mucus but structural information is scarce. Here we report the first molecular and structural characterization of a novel cell‐surface protein, Lar_0958 from Lactobacillus reuteri JCM 1112T, mediating adhesion of L. reuteri human strains to mucus. Lar_0958 is a modular protein of 133 kDa containing six repeat domains, an N‐terminal signal sequence and a C‐terminal anchoring motif (LPXTG). Lar_0958 homologues are expressed on the cell‐surface of L. reuteri human strains, as shown by flow‐cytometry and immunogold microscopy. Adhesion of human L. reuteri strains to mucus in vitro was significantly reduced in the presence of an anti‐Lar_0958 antibody and Lar_0958 contribution to adhesion was further confirmed using a L. reuteri ATCC PTA 6475 lar_0958 KO mutant (6475‐KO). The X‐ray crystal structure of a single Lar_0958 repeat, determined at 1.5 Å resolution, revealed a divergent immunoglobulin (Ig)‐like β‐sandwich fold, sharing structural homology with the Ig‐like inter‐repeat domain of internalins of the food borne pathogen Listeria monocytogenes. These findings provide unique structural insights into cell‐surface protein repeats involved in adhesion of Gram‐positive bacteria to the intestine.
Charles Peirce's Limit Concept of Truth
Authors: Legg Catherine 
Published: March 2014

AbstractThis entry explores Charles Peirce's account of truth in terms of the end or ‘limit’ of inquiry. This account is distinct from – and arguably more objectivist than – views of truth found in other pragmatists such as James and Rorty. The roots of the account in mathematical concepts is explored, and it is defended from objections that it is (i) incoherent, (ii) in its faith in convergence, too realist and (iii) in its ‘internal realism’, not realist enough.
Expression of Adiponectin and its Receptors in the Porcine Hypothalamus During the Oestrous Cycle
Authors: Kaminski T , Smolinska N , Maleszka A , Kiezun M , Dobrzyn K , Czerwinska J , Szeszko K , Nitkiewicz A 
Published: March 2014

ContentsAdiponectin is a hormonal link between obesity and reproduction, and its actions are mediated by two types of receptors: adiponectin receptor 1 (AdipoR1) and adiponectin receptor 2 (AdipoR2). This study compares the expression levels of adiponectin and adiponectin receptor mRNAs and proteins in selected areas of the porcine hypothalamus responsible for GnRH production and secretion: the mediobasal hypothalamus (MBH), pre‐optic area (POA) and stalk median eminence (SME). The tissue samples were harvested on days 2–3, 10–12, 14–16 and 17–19 of the oestrous cycle. Adiponectin mRNA expression in MBH was significantly lower on days 14–16, whereas in SME, the most pronounced gene expression was found on days 2–3 of the cycle (p < 0.05). Adiponectin protein in MBH was most abundant on days 17–19 and in POA on days 2–3 (p < 0.05). Adiponectin protein expression in SME was at similar level throughout the most of the cycle with a statistically significant drop (p < 0.05) on days 14–16. AdipoR1 gene expression in POA was potentiated on days 2–3 and 10–12 of the oestrous cycle (p < 0.05). In SME, the highest AdipoR1 mRNA expression was noted on days 2–3 (p < 0.05). The concentrations of the AdipoR1 protein in POA were similar throughout the luteal phase (days 2–14 of the cycle), and they decreased on days 17–19 (p < 0.05). In SME, AdipoR1 protein expression peak occurred on days 2–3 (p < 0.05). The expression patterns of the AdipoR2 gene in MBH, POA and SME revealed the highest mRNA levels on days 2–3 of the cycle (p < 0.05). The highest content of AdipoR2 protein in MBH was reported on days 2–3 (p < 0.05), while in POA on days 17–19 and in SME on days 10–12 and 14–16 (p < 0.05). This study demonstrated that adiponectin and adiponectin receptor mRNAs and proteins are present in the porcine hypothalamus and that their expression levels are determined by the pig's endocrine status related to the oestrous cycle.
Exploring Arts‐Based Knowledge Translation: Sharing Research Findings Through Performing the Patterns, Rehearsing the Results, Staging the Synthesis
Authors: Rieger Kendra , Schultz Annette S.H. 
Published: March 2014

ABSTRACTBackgroundCultivation of knowledge translation (KT) strategies that actively engage health professionals in critical reflection of their practice and research‐based evidence are imperative to address the research‐practice gap. While research‐based evidence is exponentially growing, our ability to facilitate uptake by nurses and other health professionals has not kept pace. Innovative approaches that extend epistemological bias beyond a singular standpoint of postpositivism, such as the utilization of arts‐based methods, expand the possibility to address the complexities of context, engage audience members, promote dissemination within communities of practice, and foster new audiences interested in research findings.AimIn this paper, we address the importance of adopting a social constructivist epistemological stance to facilitate knowledge translation to diverse audiences, explore various arts‐based knowledge translation (ABKT) strategies, and open a dialogue concerning evaluative tenets of ABKT.DiscussionABKT utilizes various art forms to disseminate research knowledge to diverse audiences and promote evidence‐informed practice. ABKT initiatives translate knowledge not based upon a linear model, which views knowledge as an objective entity, but rather operate from the premise that knowledge is socially situated, which demands acknowledging and engaging the learner within their context. Theatre, dance, photography, and poetry are art forms that are commonly used to communicate research findings to diverse audiences. Given the emerging interest and importance of utilizing this KT strategy situated within a social constructivist epistemology, potential challenges and plausible evaluative criteria specific to ABKT are presented.ConclusionABKT is an emerging KT strategy that is grounded in social constructivist epistemological tenets, and holds potential for meaningfully sharing new research knowledge with diverse audiences.Linking Evidence to ActionABKT is an innovative and synergistic approach to traditional dissemination strategies. This creative KT approach is emerging as potent transformational learning tools that are congruent with the relational nature of nursing practice. ABKT facilitates learning about new research findings in an engaging and critical reflective manner that promotes learning within communities of practice.
Draft Genome Sequence of Enterococcus faecium Strain LCT-EF301, Which Shows Changes in Biochemical Metabolism following Space Flight
Authors: Chen Zhenhong , Li Yinhu , Chang De , An Li , Guo Yinghua , Wang Junfeng , Li Tianzhi , Wang Yajuan , Zhang Xiaojun , Dai Wenkui , Liu Changting 
Published: March 2014

ABSTRACT

An
Enterococcus faecium
strain was sent into space on the Shenzhou-VIII mission. After the space flight, the strain
E. faecium
LCT-EF301 was isolated and sequenced based on the changes to its metabolic properties.
L-Plastin Is Essential for Alveolar Macrophage Production and Control of Pulmonary Pneumococcal Infection
Authors: Deady Lauren E. , Todd Elizabeth M. , Davis Chris G. , Zhou Julie Y. , Topcagic Nermina , Edelson Brian T. , Ferkol Thomas W. , Cooper Megan A. , Muenzer Jared T. , Morley Sharon Celeste 
Published: March 2014

ABSTRACT

We report that mice deficient for the hematopoietic-specific, actin-bundling protein L-plastin (LPL) succumb rapidly to intratracheal pneumococcal infection. The increased susceptibility of LPL
−/−
mice to pulmonary pneumococcal challenge correlated with reduced numbers of alveolar macrophages, consistent with a critical role for this cell type in the immediate response to pneumococcal infection. LPL
−/−
mice demonstrated a very early clearance defect, with an almost 10-fold-higher bacterial burden in the bronchoalveolar lavage fluid 3 h following infection. Clearance of pneumococci from the alveolar space in LPL
−/−
mice was defective compared to that in Rag1
−/−
mice, which lack all B and T lymphocytes, indicating that innate immunity is defective in LPL
−/−
mice. We did not identify defects in neutrophil or monocyte recruitment or in the production of inflammatory cytokines or chemokines that would explain the early clearance defect. However, efficient alveolar macrophage regeneration following irradiation required LPL. We thus identify LPL as being key to alveolar macrophage development and essential to an effective antipneumococcal response. Further analysis of LPL
−/−
mice will illuminate critical regulators of the generation of alveolar macrophages and, thus, effective pulmonary innate immunity.
Differential Induction of Apoptosis, Interferon Signaling, and Phagocytosis in Macrophages Infected with a Panel of Attenuated and Nonattenuated Poxviruses
Authors: Royo Sandra , Sainz Bruno , Hernández-Jiménez Enrique , Reyburn Hugh , López-Collazo Eduardo , Guerra Susana 
Published: March 2014

ABSTRACT
Due to the essential role macrophages play in antiviral immunity, it is important to understand the intracellular and molecular processes that occur in macrophages following infection with various strains of vaccinia virus, particularly those used as vaccine vectors. Similarities as well as differences were found in macrophages infected with different poxvirus strains, particularly at the level of virus-induced apoptosis and the expression of immunomodulatory genes, as determined by microarray analyses. Interestingly, the attenuated modified vaccinia Ankara virus (MVA) was particularly efficient in triggering apoptosis and beta interferon (IFN-β) secretion and in inducing changes in the expression of genes associated with increased activation of innate immunity, setting it apart from the other five vaccinia virus strains tested. Taken together, these results increase our understanding of how these viruses interact with human macrophages, at the cellular and molecular levels, and suggest mechanisms that may underlie their utility as recombinant vaccine vectors.

IMPORTANCE
Our studies clearly demonstrate that there are substantial biological differences in the patterns of cellular gene expression between macrophages infected with different poxvirus strains and that these changes are due specifically to infection with the distinct viruses. For example, a clear induction in IFN-β mRNA was observed after infection with MVA but not with other poxviruses. Importantly, antiviral bioassays confirmed that MVA-infected macrophages secreted a high level of biologically active type I IFN. Similarly, the phagocytic capacity of macrophages was also specifically increased after infection with MVA. Although the main scope of this study was not to test the vaccine potential of MVA as there are several groups in the field working extensively on this aspect, the characteristics/phenotypes we observed at the
in vitro
level clearly highlight the inherent advantages that MVA possesses in comparison to other poxvirus strains.
Characterization of a Novel Influenza Virus in Cattle and Swine: Proposal for a New Genus in the
Orthomyxoviridae
Family
Authors: Hause Ben M. , Collin Emily A. , Liu Runxia , Huang Bing , Sheng Zizhang , Lu Wuxun , Wang Dan , Nelson Eric A. , Li Feng 
Published: March 2014

ABSTRACT

We have recently reported the isolation of a novel virus, provisionally designated C/swine/Oklahoma/1334/2011 (C/OK), with 50% overall homology to human influenza C viruses (ICV), from a pig in Oklahoma. Deep RNA sequencing of C/OK virus found a matrix 1 (M1) protein expression strategy that differed from that of ICV. The novelty of C/OK virus prompted us to investigate whether C/OK virus could exist in a nonswine species. Significantly, we found that C/OK virus was widespread in U.S. bovine herds, as demonstrated by reverse transcription (RT)-PCR and serological assays. Genome sequencing of three bovine viruses isolated from two herds in different states further confirmed these findings. To determine whether swine/bovine C/OK viruses can undergo reassortment with human ICV, and to clarify the taxonomic status of C/OK,
in vitro
reassortment and serological typing by agar gel immunodiffusion (AGID) were conducted.
In vitro
reassortment using two human ICV and two swine and bovine C/OK viruses demonstrated that human ICV and C/OK viruses were unable to reassort and produce viable progeny. Antigenically, no cross-recognition of detergent split virions was observed in AGID between human and nonhuman viruses by using polyclonal antibodies that were reactive to cognate antigens. Taken together, these results demonstrate that C/OK virus is genetically and antigenically distinct from ICV. The classification of the new virus in a separate genus of the
Orthomyxoviridae
family is proposed. The finding of C/OK virus in swine and bovine indicates that this new virus may spread and establish infection in other mammals, including humans.

IMPORTANCE
Influenza C viruses (ICV) are common human pathogens, infecting most people during childhood and adolescence, and typically cause mild respiratory symptoms. While ICV have been isolated from both pigs and dogs, humans are thought to be the natural viral reservoir. Previously, we characterized an ICV-like virus isolated from pigs exhibiting symptoms of influenza virus-like illness. Here, we show molecular and serological data demonstrating widespread circulation of similar viruses in bovines. Deep RNA sequencing, phylogenetic analysis, and
in vitro
reassortment experiments demonstrate that animal ICV-like viruses are genetically distinct from human ICV. Antigenically, we show that ICV-like viruses are not recognized by ICV antibodies. En masse, these results suggest that bovine influenza virus warrants classification as a new genus of influenza virus. The finding of this novel virus that can infect multiple mammalian species warrants further research into its role in human health.
Use and Misuse of Statistical Significance in Survival Analyses
Authors: Furuya Yoichi , Wijesundara Danushka K. , Neeman Teresa , Metzger Dennis W. 
Published: March 2014

ABSTRACT
In most preclinical disease models, survival analyses are the gold standard for measuring the efficacy of medical interventions such as therapeutics or vaccines. In these analyses, treatment regimens that promote the survival and/or reduce the morbidity of experimental subjects (e.g., mice) are tested for efficacy. Although these analyses appear to be relatively straightforward, there are associated caveats regarding interpretation of the results that we wish to discuss in this editorial. Of particular concern is overinterpretation of the biological significance of survival data based on statistical significance rather than durability of protection.