bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robotising vitreoretinal surgeries
Authors: Mi Helen , MacLaren Robert E. , Cehajic-Kapetanovic Jasmina 
Published: July 2024

AbstractThe use of robotic surgery in ophthalmology has been shown to offer many potential advantages to current surgical techniques. Vitreoretinal surgery requires complex manoeuvres and high precision, and this is an area that exceeds manual human dexterity in certain surgical situations. With the advent of advanced therapeutics such as subretinal gene therapy, precise delivery and minimising trauma is imperative to optimize outcomes. There are multiple robotic systems in place for ophthalmology in pre-clinical and clinical use, and the Preceyes Robotic Surgical System (Preceyes BV) has also gained the CE mark and is commercially available for use. Recent in-vivo and in-human surgeries have been performed successfully with robotics systems. This includes membrane peeling, subretinal injections of therapeutics, and retinal vein cannulation. There is huge potential to integrate robotic surgery into mainstream clinical practice. In this review, we summarize the existing systems, and clinical implementation so far, and highlight the future clinical applications for robotic surgery in vitreo-retina.
Contextual quantum metrology
Authors: Jae Jeongwoo , Lee Jiwon , Kim M. S. , Lee Kwang-Geol , Lee Jinhyoung 
Published: July 2024

AbstractWe demonstrate that the contextuality of measurement selection can enhance the precision of quantum metrology with a simple linear optical experiment. Contextuality is a nonclassical property known as a resource for various quantum information processing tasks. Recent studies show that contextuality by anomalous weak values can be utilized to enhance metrological precision, unraveling the role of contextuality in quantum metrology. Our contextual quantum metrology (coQM) scheme can elevate the precision of the optical polarimetry as much as 6 times the precision limit given by the Quantum Fisher Information. We achieve the contextuality-enabled enhancement with two mutually complementary measurements, whereas, in the conventional method, some optimal measurements to achieve the precision limit are either theoretically challenging to find or experimentally infeasible to realize. These results highlight that the contextuality of measurement selection is applicable in practice for quantum metrology.
Principled distillation of UK Biobank phenotype data reveals underlying structure in human variation
Authors: Carey Caitlin E. , Shafee Rebecca , Wedow Robbee , Elliott Amanda , Palmer Duncan S. , Compitello John , Kanai Masahiro , Abbott Liam , Schultz Patrick , Karczewski Konrad J. , Bryant Samuel C. , Cusick Caroline M. , Churchhouse Claire , Howrigan Daniel P. , King Daniel , Davey Smith George , Neale Benjamin M. , Walters Raymond K. , Robinson Elise B. 
Published: July 2024

AbstractData within biobanks capture broad yet detailed indices of human variation, but biobank-wide insights can be difficult to extract due to complexity and scale. Here, using large-scale factor analysis, we distill hundreds of variables (diagnoses, assessments and survey items) into 35 latent constructs, using data from unrelated individuals with predominantly estimated European genetic ancestry in UK Biobank. These factors recapitulate known disease classifications, disentangle elements of socioeconomic status, highlight the relevance of psychiatric constructs to health and improve measurement of pro-health behaviours. We go on to demonstrate the power of this approach to clarify genetic signal, enhance discovery and identify associations between underlying phenotypic structure and health outcomes. In building a deeper understanding of ways in which constructs such as socioeconomic status, trauma, or physical activity are structured in the dataset, we emphasize the importance of considering the interwoven nature of the human phenome when evaluating public health patterns.
Renal autocrine neuropeptide FF (NPFF) signaling regulates blood pressure
Authors: Lee Hewang , Amatya Bibhas , Villar Van Anthony M. , Asico Laureano D. , Jeong Jin Kwon , Feranil Jun , Moore Shaun C. , Zheng Xiaoxu , Bishop Michael , Gomes Jerald P. , Polzin Jacob , Smeriglio Noah , de Castro Pedro A. S. Vaz , Armando Ines , Felder Robin A. , Hao Ling , Jose Pedro A. 
Published: July 2024

AbstractThe kidney and brain play critical roles in the regulation of blood pressure. Neuropeptide FF (NPFF), originally isolated from the bovine brain, has been suggested to contribute to the pathogenesis of hypertension. However, the roles of NPFF and its receptors, NPFF-R1 and NPFF-R2, in the regulation of blood pressure, via the kidney, are not known. In this study, we found that the transcripts and proteins of NPFF and its receptors, NPFF-R1 and NPFF-R2, were expressed in mouse and human renal proximal tubules (RPTs). In mouse RPT cells (RPTCs), NPFF, but not RF-amide-related peptide-2 (RFRP-2), decreased the forskolin-stimulated cAMP production in a concentration- and time-dependent manner. Furthermore, dopamine D1-like receptors colocalized and co-immunoprecipitated with NPFF-R1 and NPFF-R2 in human RPTCs. The increase in cAMP production in human RPTCs caused by fenoldopam, a D1-like receptor agonist, was attenuated by NPFF, indicating an antagonistic interaction between NPFF and D1-like receptors. The renal subcapsular infusion of NPFF in C57BL/6 mice decreased renal sodium excretion and increased blood pressure. The NPFF-mediated increase in blood pressure was prevented by RF-9, an antagonist of NPFF receptors. Taken together, our findings suggest that autocrine NPFF and its receptors in the kidney regulate blood pressure, but the mechanisms remain to be determined.
Experimental study on the mechanical characteristics of weakly cemented mudstone under different loading rates
Authors: Zou Junpeng , Li Gang , Li Zibo , Zhang Yabing , Liu Hang , Wang Yiming 
Published: July 2024

AbstractWith the gradual shift of coal mining to the western coal mining region of China, floor heave in weakly cemented mudstone roadways has become an issue affecting the safety and efficiency of coal mine production. Additionally, different mining rates can lead to fluctuating support stresses on the roof and floor of weakly cemented mudstone roadways. Therefore, obtaining a comprehensive understanding of the mechanical properties of weakly cemented mudstone at different loading rates is conducive to improving the issue of floor heave in such roadways and provides a theoretical basis for further study. In this context, a series of uniaxial mechanical tests with concurrent acoustic emission monitoring were conducted on specimens of weakly cemented mudstone under various loading rates (0.005, 0.01, 0.05, and 0.1 mm/s). The stress‒strain and acoustic emission response curves were obtained to effectively characterize the strength, deformation, damage, macroscale instability, and crack propagation characteristics of the mudstone under the influence of loading rate effects. The research results support the following findings: (1) With increasing loading rate, the peak strength and elastic modulus of weakly cemented mudstone significantly increase, while the peak axial strain and peak radial deformation significantly decrease. (2) With increasing loading rate, the stress required to trigger the expansion of weakly cemented mudstone gradually increases, and a significant power-law relationship arises between the strain of the mudstone at the start of expansion and the loading rate. (3) With increasing loading rate, the acoustic emission ringing count of weakly cemented mudstone increases: The failure of weakly cemented mudstone changes from small-range progressive failure to sudden failure, and the failure mode transitions from shear failure to tensile‒shear composite failure. (4) The studied mudstone damage variables increase with increasing loading rate, following an approximate exponential function. The conclusions obtained in this work can provide a theoretical basis for the evolution mechanism and control of floor heave in deep roadway mining.
Association of modifiable metabolic risk factors and lifestyle with all-cause mortality in patients with hepatocellular carcinoma
Authors: Kim Hwi Young , Lee Hye Ah , Radu Pompilia , Dufour Jean-François 
Published: July 2024

AbstractWe aimed to investigate the potential impact of metabolic risk factors and lifestyles on mortality in hepatocellular carcinoma (HCC) patients. From the Korean Central Cancer Registry database (2008–2016), 8,505 HCC patients were included in the analysis. Patients with 2 or more metabolic risk factors (n = 2384, 28.0%) showed significantly worse overall survival (OS, 29 months, 95% confidence interval [CI] 27–33) than patients with 0 (n = 2269 [26.7%]; 41 months, 95% CI 37–47), or 1 (n = 3852 [45.3%]; 42 months; 95% CI 38–46) metabolic risk factor. (P < 0.001) In the multivariable Cox analysis, patients with ≥ 2 metabolic risk factors had significantly elevated risk of overall mortality (adjusted hazards ratio (HR) = 1.14 [95% CI 1.06–1.23], P < 0.001) and HCC-specific mortality (sub-distribution HR = 1.09 [95% CI 1.00–1.09], P = 0.046), compared to those without. Alcohol and smoking were also independent risk factors for worse overall and HCC-specific mortality (all P < 0.05). Metabolic comorbidities were associated with greater risk of mortality in a dose-dependent manner in HCC patients, regardless of tumor stage and liver function. Alcohol intake and smoking significantly increased mortality by themselves and even further with the presence of metabolic risk.
Comprehensive application of AI algorithms with TCR NGS data for glioma diagnosis
Authors: Zhou Kaiyue , Xiao Zhengliang , Liu Qi , Wang Xu , Huo Jiaxin , Wu Xiaoqi , Zhao Xiaoxiao , Feng Xiaohan , Fu Baoyi , Xu Pengfei , Deng Yunyun , Xiao Wenwen , Sun Tao , Da Lin 
Published: July 2024

AbstractT-cell receptor (TCR) detection can examine the extent of T-cell immune responses. Therefore, the article analyzed characteristic data of glioma obtained by DNA-based TCR high-throughput sequencing, to predict the disease with fewer biomarkers and higher accuracy. We downloaded data online and obtained six TCR-related diversity indices to establish a multidimensional classification system. By comparing actual presence of the 602 correlated sequences, we obtained two-dimensional and multidimensional datasets. Multiple classification methods were utilized for both datasets with the classification accuracy of multidimensional data slightly less to two-dimensional datasets. This study reduced the TCR β sequences through feature selection methods like RFECV (Recursive Feature Elimination with Cross-Validation). Consequently, using only the presence of these three sequences, the classification AUC value of 96.67% can be achieved. The combination of the three correlated TCR clones obtained at a source data threshold of 0.1 is: CASSLGGNTEAFF_TRBV12_TRBJ1-1, CASSYSDTGELFF_TRBV6_TRBJ2-2, and CASSLTGNTEAFF_TRBV12_TRBJ1-1. At 0.001, the combination is: CASSLGETQYF_TRBV12_TRBJ2-5, CASSLGGNQPQHF_TRBV12_TRBJ1-5, and CASSLSGNTIYF_TRBV12_TRBJ1-3. This method can serve as a potential diagnostic and therapeutic tool, facilitating diagnosis and treatment of glioma and other cancers.
Enhancing drought stress tolerance and growth promotion in chiltepin pepper (Capsicum annuum var. glabriusculum) through native Bacillus spp.
Authors: Mendoza-Alatorre Maribel , Infante-Ramírez Rocío , González-Rangel María Olga , Nevárez-Moorillón Guadalupe Virginia , González-Horta María del Carmen , Hernández-Huerta Jared , Delgado-Gardea Ma. Carmen E. 
Published: July 2024

AbstractThe drought can cause a decrease in food production and loss of biodiversity. In northern Mexico, an arid region, the chiltepin grows as a semi-domesticated crop that has been affected in its productivity and yield. An alternative to mitigate the effect of drought and aid in its conservation could be using Plant Growth-Promoting Bacteria (PGPB). The present study evaluated the capacity of native Bacillus spp., isolated from arid soils, as PGPBs and drought stress tolerance inducers in chiltepin under controlled conditions. Chiltepin seeds and seedlings were inoculated with native strains of Bacillus spp. isolated from arid soils, evaluating germination, vegetative, and drought stress tolerance parameters. The PGPBs improved vegetative parameters such as height, stem diameter, root length, and slenderness index in vitro. B. cereus (Bc25-7) improved in vitro survival of stressed seedlings by 68% at −1.02 MPa. Under greenhouse conditions, seedlings treated with PGPBs exhibited increases in root length (9.6%), stem diameter (13.68%), leaf fresh weight (69.87%), and chlorophyll content (38.15%). Bc25-7 alleviated severe water stress symptoms (7 days of water retention stress), and isolates B. thuringiensis (Bt24-4) and B. cereus (Bc25-7, and Bc30-2) increased Relative Water Content (RWC) by 51%. Additionally, the treated seeds showed improved germination parameters with a 46.42% increase in Germination Rate (GR). These findings suggest that using PGPBs could be an alternative to mitigate the effect of drought on chiltepin.
A single-cell transcriptomic study of heterogeneity in human embryonic tanycytes
Authors: Bai Yiguang , Chen Qiaoling , Li Yuan 
Published: July 2024

AbstractDisruptions in energy homeostasis can lead to diseases like obesity and diabetes, affecting millions of people each year. Tanycytes, the adult stem cells in the hypothalamus, play crucial roles in assisting hypothalamic neurons in maintaining energy balance. Although tanycytes have been extensively studied in rodents, our understanding of human tanycytes remains limited. In this study, we utilized single-cell transcriptomics data to explore the heterogeneity of human embryonic tanycytes, investigate their gene regulatory networks, analyze their intercellular communication, and examine their developmental trajectory. Our analysis revealed the presence of two clusters of β tanycytes and three clusters of α tanycytes in our dataset. Surprisingly, human embryonic tanycytes displayed significant similarities to mouse tanycytes in terms of marker gene expression and transcription factor activities. Trajectory analysis indicated that α tanycytes were the first to be generated, giving rise to β tanycytes in a dorsal–ventral direction along the third ventricle. Furthermore, our CellChat analyses demonstrated that tanycytes generated earlier along the developmental lineages exhibited increased intercellular communication compared to those generated later. In summary, we have thoroughly characterized the heterogeneity of human embryonic tanycytes from various angles. We are confident that our findings will serve as a foundation for future research on human tanycytes.
Biostimulant and antagonistic potential of endophytic fungi against fusarium wilt pathogen of tomato Fusarium oxysporum f. sp. lycopersici
Authors: Muhorakeye Marie Cecile , Namikoye Everlyne Samita , Khamis Fathiya M. , Wanjohi Waceke , Akutse Komivi S. 
Published: July 2024

AbstractEndophytic fungal-based biopesticides are sustainable and ecologically-friendly biocontrol agents of several pests and diseases. However, their potential in managing tomato fusarium wilt disease (FWD) remains unexploited. This study therefore evaluated effectiveness of nine fungal isolates against tomato fusarium wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL) in vitro using dual culture and co-culture assays. The efficacy of three potent endophytes that inhibited the pathogen in vitro was assessed against FWD incidence, severity, and ability to enhance growth and yield of tomatoes in planta. The ability of endophytically-colonized tomato (Solanum lycopersicum L.) plants to systemically defend themselves upon exposure to FOL were also assessed through defence genes expression using qPCR. In vitro assays showed that endophytes inhibited and suppressed FOL mycelial growth better than entomopathogenic fungi (EPF). Endophytes Trichoderma asperellum M2RT4, Hypocrea lixii F3ST1, Trichoderma harzianum KF2R41, and Trichoderma atroviride ICIPE 710 had the highest (68.84–99.61%) suppression and FOL radial growth inhibition rates compared to EPF which exhibited lowest (27.05–40.63%) inhibition rates. Endophytes T. asperellum M2RT4, H. lixii F3ST1 and T. harzianum KF2R41 colonized all tomato plant parts. During the in planta experiment, endophytically-colonized and FOL-infected tomato plants showed significant reduction of FWD incidence and severity compared to non-inoculated plants. In addition, these endophytes contributed to improved growth promotion parameters and yield. Moreover, there was significantly higher expression of tomato defence genes in T. asperellum M2RT4 colonized than in un-inoculated tomato plants. These findings demonstrated that H. lixii F3ST1 and T. asperellum M2RT4 are effective biocontrol agents against FWD and could sustainably mitigate tomato yield losses associated with fusarium wilt.
Characterization, comparison, and phylogenetic analyses of chloroplast genomes of Euphorbia species
Authors: Lee Soo-Rang , Oh Ami , Son Dong Chan 
Published: July 2024

AbstractThe genus Euphorbia (Euphorbiaceae) has near-cosmopolitan distribution and serves as a significant resource for both ornamental and medicinal purposes. Despite its economic importance, Euphorbia's taxonomy has long been challenged by the intricate nature of morphological traits exhibiting high levels of convergence. While molecular markers are essential for phylogenetic studies, their availability for Euphorbia has been limited. To address this gap, we conducted comparative analyses focusing on the chloroplast (CP) genomes of nine Euphorbia species, incorporating three newly sequenced and annotated accessions. In addition, phylogenetic informativeness and nucleotide diversity were computed to identify candidate markers for phylogenetic analyses among closely related taxa in the genus. Our investigation revealed relatively conserved sizes and structures of CP genomes across the studied species, with notable interspecific variations observed primarily in non-coding regions and IR/SC borders. By leveraging phylogenetic informativeness and nucleotide diversity, we identified rpoB gene as the optimal candidate for species delimitation and shallow-level phylogenetic inference within the genus. Through this comprehensive analysis of CP genomes across multiple taxa, our study sheds light on the evolutionary dynamics and taxonomic intricacies of Euphorbia, offering valuable insights into its CP genome evolution and taxonomy.
Determination of operation performance indicators of unit for mowing crops with the simultaneous incorporation of their stubble into the soil
Authors: Nadykto Volodymyr , Golub Gennadii , Kukharets Savelii , Kyurchev Volodymyr , Skliar Oleksandr , Hutsol Taras , Glowacki Szymon , Nurek Tomasz , Horetska Iryna , Yakovenko Anatoly 
Published: July 2024

AbstractWhen harvesting grain crops and forage grasses using a two-phase method, trailed and/or mounted windrowers are usually used. After their passage, stubble remains on the field, which intensively loses soil moisture under sunlight and wind. To reduce these losses, the stubble, along with the soil, is crushed using disc harrows. Due to the use of two sequentially operating units (harvesting and soil-cultivating), their total operating time increases. This does little to preserve soil moisture in the cultivated field. This article provides an example of a more effective solution to this problem. It consists of using one machine-tractor unit instead of two. The proposed combined unit mows an agricultural crop in one working pass and ensures stubble crushing and incorporation into the top layer of soil. The unit consists of a wheeled tractor with a front hitch linkage, a front windrower and a disc harrow mounted behind the tractor. It has been established that the laboriousness of compiling such a unit, considering the tractor's transformation to reverse, is insignificant and amounts to 1442 person-hours. The use of the new unit assists in reducing soil moisture losses. Over a month, it can reach 4.1–5.2% in absolute terms and 15–45% in relative ones. The combined unit movement velocity should be close to 2.5 m s−1 to ensure such a reduction in soil moisture losses. Combining two technological operations performed by one machine-tractor unit does not impair its reliability. At the same time, there is a reduction in processing time for one field by almost half and a decrease in fuel consumption per unit of performed area by 2.25 times.
Patient self-inflicted lung injury associated pneumothorax/pneumomediastinum is a risk factor for worse outcomes of severe COVID-19: a case-control study
Authors: Cai Zhigui , Guo Xingxing , Lv Xing , Wu Yunfu , Niu Xiaona , Song Liqiang 
Published: July 2024

AbstractWe aimed to determine the clinical characteristics of patient self-inflicted lung injury (P-SILI)-associated pneumothorax/pneumomediastinum, to reveal its risk factors, and to assess its impact on severe COVID-19 cases. In total, 229 patients were included in this case-control study. They were randomly divided into either the case group or the control group as per the inclusion and exclusion criteria. The two groups were further analyzed to reveal the risk factors of spontaneous pneumothorax/pneumomediastinum (SP/P). Finally, risk factors for death were analyzed in the case group and the relationship between death and SP/P was also analyzed among all patients. The mean age of patients was 59.69 ± 17.01 years, most of them were male (74.2%), and 62.0% of them had comorbidities upon admission. A respiratory rate higher than 30 BPM was a risk factor for SP/P (OR 7.186, 95% CI 2.414–21.391, P < 0.001). Patients with delayed intubation due to early application of HFNC or NIV had a higher mortality rate when they developed SP/P (P < 0.05). Additionally, advanced age increased the risk of death (P < 0.05). Finally, SP/P may be a risk factor for death among patients with severe COVID-19 (OR 2.047). P-SILI occurs in severe COVID-19 with acute respiratory failure. It is necessary to identify the risk factors of P-SILI, the indicators of severe P-SILI, and the preventive measures.