bluuebunny/crossref_metadata_2025_split huggingface dataset:
Markers of iron metabolism in retired racing Greyhounds with and without osteosarcoma
Authors: Caro J. T. , Marín L. M. , Iazbik M. C. , Zaldivar‐López S. , Borghese H. , Couto C. G. 
Published: September 2013

BackgroundGreyhounds have well‐described clinicopathologic idiosyncrasies, including a high prevalence of osteosarcoma (OSA). Hematocrit, HGB, and HGB oxygen affinity are higher than in other dogs, while haptoglobin concentration is lower, so we hypothesized that Greyhounds have a different iron metabolism. To our knowledge, there are no reports on serum iron profiles in Greyhounds.ObjectivesTo elucidate iron metabolism in Greyhounds, we wanted to compare serum iron concentration, total iron‐binding capacity (TIBC), and percent transferrin saturation (%SAT) in healthy retired racing Greyhounds (RRGs) with OSA (RRGs –OSA), and also with non‐Greyhounds (NGs), without and withOSA(NGs –OSA).MethodsSerum iron concentration and unsaturated iron‐binding capacity (UIBC) were measured by standard methods, andTIBCand %SATwere calculated inRRGs (n = 25),RRGs –OSA(n = 28),NGs (n = 30), andNGs –OSA(n = 32).ResultsTIBCwas lower inRRGs than inNGs (P < .0001), and inRRGs –OSAthan inNGs –OSA(P < .0001).NGs –OSAhad lowerTIBCthan healthyNGs (P = .003). PercentSATwas higher inRRGs than inNGs (P < .0001) and inRRGs –OSA(P = .008), and %SATwas also lower inNGs than inNGs –OSA(P = .004). PercentSATwas also higher inRRGs –OSAthan inNGs –OSA(P = .001). BothRRGs –OSA(P = .02) andNGs –OSA(P < .0001) had lower serum iron concentrations than their healthy counterparts.ConclusionLowerTIBCand higher %SATmay constitute another Greyhound idiosyncrasy compared with other dogs. In this study, all dogs withOSAhad higher serum iron concentrations and %SATthan healthy dogs.
Low dose tomographic fluoroscopy: 4D intervention guidance with running prior
Authors: Flach Barbara , Kuntz Jan , Brehm Marcus , Kueres Rolf , Bartling Sönke , Kachelrieß Marc 
Published: September 2013

Purpose:Todayˈs standard imaging technique in interventional radiology is the single‐ or biplane x‐ray fluoroscopy which delivers 2D projection images as a function of time (2D+T). This state‐of‐the‐art technology, however, suffers from its projective nature and is limited by the superposition of the patientˈs anatomy. Temporally resolved tomographic volumes (3D+T) would significantly improve the visualization of complex structures. A continuous tomographic data acquisition, if carried out with todayˈs technology, would yield an excessive patient dose. Recently the authors proposed a method that enables tomographic fluoroscopy at the same dose level as projective fluoroscopy which means that if scanning time of an intervention guided by projective fluoroscopy is the same as that of an intervention guided by tomographic fluoroscopy, almost the same dose is administered to the patient. The purpose of this work is to extend authorsˈ previous work and allow for patient motion during the intervention.Methods:The authors propose the running prior technique for adaptation of a prior image. This adaptation is realized by a combination of registration and projection replacement. In a first step the prior is deformed to the current position via affine and deformable registration. Then the information from outdated projections is replaced by newly acquired projections using forward and backprojection steps. The thus adapted volume is the running prior. The proposed method is validated by simulated as well as measured data. To investigate motion during intervention a moving head phantom was simulated. Realin vivo data of a pig are acquired by a prototype CT system consisting of a flat detector and a continuously rotating clinical gantry.Results:With the running prior technique it is possible to correct for motion without additional dose. For an application in intervention guidance both steps of the running prior technique, registration and replacement, are necessary. Reconstructed volumes based on the running prior show high image quality without introducing new artifacts and the interventional materials are displayed at the correct position.Conclusions:The running prior improves the robustness of low dose 3D+T intervention guidance toward intended or unintended patient motion.
Use of dMLC for implementation of dynamic respiratory‐gated radiation therapy
Authors: Pepin Eric W. , Wu Huanmei , Shirato Hiroki 
Published: September 2013

Purpose:To simulate and evaluate the use of dynamic multileaf collimators (dMLC) in respiratory gating to compensate for baseline drift.Methods:Tumor motion tracking data from 30 lung tumors over 322 treatment fractions was analyzed with the finite state model. A dynamic respiratory gating window was established in real‐time by determining the average positions during the previous two end‐of‐expiration breathing phases and centering the dMLC aperture on a weighted average of these positions. A simulated dMLC with physical motion constraints was used in dynamic gating treatment simulations. Fluence maps were created to provide a statistical description of radiation delivery for each fraction. Duty cycle was also calculated for each fraction.Results:The average duty cycle was 2.3% greater under dynamic gating conditions. Dynamic gating also showed higher fluences and less tumor obstruction. Additionally, dynamic gating required fewer beam toggles and each delivery period was longer on average than with static gating.Conclusions:The use of dynamic gating showed better performance than static gating and the physical constraints of a dMLC were shown to not be an impediment to dynamic gating.
Simultaneous 99mTc‐MDP/123I‐MIBG tumor imaging using SPECT‐CT: Phantom and constructed patient studies
Authors: Rakvongthai Yothin , Fakhri Georges El , Lim Ruth , Bonab Ali A. , Ouyang Jinsong 
Published: September 2013

Purpose:Authors’ goal is to evaluate the performance of simultaneous99mTc‐MDP/123I‐MIBG tumor imaging with fast Monte‐Carlo (MC) based joint iterative reconstruction as compared to sequential 99mTc‐MDP and 123I‐MIBG tumor imaging.Methods:Noise‐free99mTc and 123I SPECT projections were acquired separately using an anthropomorphic torso phantom modified to include a fillable tube around the lungs to mimic ribs. Additionally, 99mTc and 123I projections were acquired separately using a 1‐cm spherical “tumor” placed at various distances from one detector. Tumor‐present data were generated by adding tumor projections to the torso phantom data, which were scaled to the total counts in typical clinical studies. Twenty‐five noise realizations were generated by adding Poisson noise to the projection data for each radionuclide. Dual‐radionuclide data were synthesized by summing the 99mTc and 123I projections. Image reconstruction was performed using: (1) SR‐OSEM, ordered subset expectation maximization (OSEM) without scatter correction (SC) using single‐radionuclide (SR) data; (2) SR‐MC‐OSEM, OSEM with a fast MC‐based SC using SR data; (3) DR‐OSEM, OSEM without SC using dual‐radionuclide (DR) data; and (4) DR‐MC‐JOSEM, joint OSEM with a fast MC‐based SC using DR data. Ten 99mTc‐MDP and ten 123I‐MIBG data sets, which had tumors mathematically inserted, were also used to evaluate the performance of authors’ approach. For the phantom study, relative bias and relative standard deviation of tumor uptake were computed for each tumor using the tumor uptake in the noise‐free single‐radionuclide images, which were reconstructed by SR‐MC‐OSEM, as the gold standard. For both the phantom and constructed patient studies, mean contrast and standard deviation of contrast were computed for each tumor for both the single‐ and dual‐radionuclide images. Additionally, contrast recovery was computed as the ratio between mean contrast and the mean contrast for SR‐MC‐OSEM.Results:For the phantom study, DR‐MC‐JOSEM yielded 2.7% on average relative bias of tumor uptake using the images, which were reconstructed from the noise‐free SR data with SR‐MC‐OSEM, as the gold‐standard. For both the phantom and constructed patient studies, DR‐MC‐JOSEM yielded 94.7% and 95.2% tumor contrast recovery on average using SR‐MC‐OSEM as the reference, in the phantom and constructed patient studies, respectively. DR‐MC‐JOSEM yielded comparable relative standard deviation of bias and standard deviation of contrast to SR‐MC‐OSEM.Conclusions:Simultaneous99mTc‐MDP/123I‐MIBG tumor imaging using authors’ dual‐radionuclide reconstruction approach yielded comparable image quality to sequential 99mTc‐MDP and 123I‐MIBG imaging. For patients who need to undergo both scans, authors’ approach offers perfectly registered dual‐tracer images under identical conditions without compromising image quality, and reduces the imaging cost while increasing patient throughput.
Complete Genome Sequence of SfII, a Serotype-Converting Bacteriophage of the Highly Prevalent Shigella flexneri Serotype 2a
Authors: George Divya T. , Stephenson David P. , Tran Elizabeth , Morona Renato , Verma Naresh K. 
Published: September 2013

ABSTRACT

SfII is a serotype-converting temperate bacteriophage of the highly prevalent
Shigella flexneri
serotype 2a. We isolated the SfII phage from a wild-type strain of
S. flexneri
serotype 2a. Here, we present the complete genome sequence of this phage.
Draft Genome Sequence of the Cellulolytic Bacterium Clostridium papyrosolvens C7 (ATCC 700395)
Authors: Zepeda Veronica , Dassa Bareket , Borovok Ilya , Lamed Raphael , Bayer Edward A. , Cate Jamie H. D. 
Published: September 2013

ABSTRACT

We report the draft genome sequence of the cellulose-degrading bacterium
Clostridium papyrosolvens
C7, originally isolated from mud collected below a freshwater pond in Massachusetts. This Gram-positive bacterium grows in a mesophilic anaerobic environment with filter paper as the only carbon source, and it has a simple cellulosome system with multiple carbohydrate-degrading enzymes.
Genome Sequence of the Bacterium Bifidobacterium longum Strain CMCC P0001, a Probiotic Strain Used for Treating Gastrointestinal Disease
Authors: Yu Hongjing , Liu Lu , Chang Zhen , Wang Shasha , Wen Bin , Yin Peijun , Liu Datao , Chen Bei , Zhang Jundong 
Published: September 2013

ABSTRACT

Bifidobacterium longum
subsp.
longum
CMCC P0001, a standard probiotic strain in China, has been widely used in clinical medicine for more than 20 years. Here we report the genome features of
B. longum
strain CMCC P0001.
Draft Genome Sequence of Entomopathogenic Bacterium Photorhabdus temperata Strain M1021, Isolated from Nematodes
Authors: Park Gun-Seok , Khan Abdur Rahim , Hong Sung-Jun , Jang Eun-Kyung , Ullah Ihsan , Jung Byung Kwon , Choi JungBae , Yoo Na-Kyung , Park Keun-Joon , Shin Jae-Ho 
Published: September 2013

ABSTRACT

Photorhabdus temperata
strain M1021 is an entomopathogenic bacterium belonging to the family
Enterobacteriaceae
and is symbiotically associated with nematodes. The draft genome sequence of
P. temperata
strain M1021 consists of 5,598,253 bp with a G+C content of 43.7%, and it has 6,120 protein-coding genes.
Effects of Acupuncture on the Symptoms of Anxiety and Depression Caused by Premenstrual Dysphoric Disorder
Authors: Carvalho Fabiana , Weires Kelly , Ebling Márcia , de Souza Rabbo Padilha Maristela , Ferrão Ygor Arzeno , Vercelino Rafael 
Published: September 2013

Objective The objective of this investigation was to evaluate the effects of acupuncture and sham acupuncture on the symptoms of anxiety and depression brought on by premenstrual dysphoric disorder (PMDD). Methods In a single-blind randomised clinical trial, 30 volunteers with PMDD were assigned alternately to group 1 (acupuncture) or group 2 (sham acupuncture), and completed an evaluation of symptoms of anxiety and depression using the Hamilton Anxiety (HAM-A) and Hamilton Depression (HAM-D) Rating Scales. The procedure was performed twice a week for two menstrual cycles, for a total of 16 attendances for each participant. Results Before the intervention the mean HAM-A and HAM-D scores did not differ between groups. Following the intervention symptoms of anxiety and depression were reduced in both groups; however, the improvement was significant in group 1 compared to group 2, as shown by a mean reduction in HAM-A scores of 58.9% in group 1 and 21.2% in group 2 (p<0.001). The reduction in the mean HAM-D scores was 52.0% in group 1 and 19.6% in group 2, resulting in a significant difference (p=0.012). Conclusions The results suggest that acupuncture could be another treatment option for PMDD patients.
Combined Immunostimulatory Monoclonal Antibodies Extend Survival in an Aggressive Transgenic Hepatocellular Carcinoma Mouse Model
Authors: Morales-Kastresana Aizea , Sanmamed Miguel F. , Rodriguez Inmaculada , Palazon Asis , Martinez-Forero Ivan , Labiano Sara , Hervas-Stubbs Sandra , Sangro Bruno , Ochoa Carmen , Rouzaut Ana , Azpilikueta Arantza , Bolaños Elixabet , Jure-Kunkel Maria , Gütgemann Ines , Melero Ignacio 
Published: September 2013

AbstractPurpose: Immunostimulatory monoclonal antibodies (ISmAb) that unleash antitumor immune responses are showing efficacy in cancer clinical trials. Anti-B7-H1 (PD-L1) monoclonal antibodies (mAb) block a critical inhibitory pathway in T cells, whereas anti-CD137 and OX40 mAbs provide T-cell costimulation. A combination of these ISmAbs (anti-CD137 + anti-OX40 + anti-B7-H1) was tested using a transgenic mouse model of multifocal and rapidly progressing hepatocellular carcinoma, in which c-myc drives transformation and cytosolic ovalbumin (OVA) is expressed in tumor cells as a model antigen.Experimental Design: Flow-cytometry and immunohistochemistry were used to quantify tumor-infiltrating lymphocytes (TIL) elicited by treatment and assess their activation status and cytolytic potential. Tolerance induction and its prevention/reversal by treatment with the combination of ISmAbs were revealed by in vivo killing assays.Results: The triple combination of ISmAbs extended survival of mice bearing hepatocellular carcinomas in a CD8-dependent fashion and synergized with adoptive T-cell therapy using activated OVA-specific TCR-transgenic OT-1 and OT-2 lymphocytes. Mice undergoing therapy showed clear increases in tumor infiltration by activated and blastic CD8+ and CD4+ T lymphocytes containing perforin/granzyme B and expressing the ISmAb-targeted receptors on their surface. The triple combination of ISmAbs did not result in enhanced OVA-specific cytotoxic T lymphocyte (CTL) activity but other antigens expressed by cell lines derived from such hepatocellular carcinomas were recognized by endogenous TILs. Adoptively transferred OVA-specific OT-1 lymphocytes into tumor-bearing mice were rendered tolerant, unless given the triple mAb therapy.Conclusion: Extension of survival and dense T-cell infiltrates emphasize the translational potential of combinational immunotherapy strategies for hepatocellular carcinoma. Clin Cancer Res; 19(22); 6151–62. ©2013 AACR.
The Cannabinoid WIN 55,212-2 Decreases Specificity Protein Transcription Factors and the Oncogenic Cap Protein eIF4E in Colon Cancer Cells
Authors: Sreevalsan Sandeep , Safe Stephen 
Published: September 2013

Abstract2,3-Dihydro-5-methyl-3-([morpholinyl]methyl)pyrollo(1,2,3-de)-1,4-benzoxazinyl]-[1-naphthaleny]methanone [WIN 55,212-2, (WIN)] is a synthetic cannabinoid that inhibits RKO, HT-29, and SW480 cell growth, induced apoptosis, and downregulated expression of survivin, cyclin D1, EGF receptor (EGFR), VEGF, and its receptor (VEGFR1). WIN also decreased expression of specificity protein (Sp) transcription factors Sp1, Sp3, and Sp4, and this is consistent with the observed downregulation of the aforementioned Sp-regulated genes. In addition, we also observed by RNA interference (RNAi) that the oncogenic cap protein eIF4E was an Sp-regulated gene also downregulated by WIN in colon cancer cells. WIN-mediated repression of Sp proteins was not affected by cannabinoid receptor antagonists or by knockdown of the receptor but was attenuated by the phosphatase inhibitor sodium orthovanadate or by knockdown of protein phosphatase 2A (PP2A). WIN-mediated repression of Sp1, Sp3, and Sp4 was due to PP2A-dependent downregulation of microRNA-27a (miR-27a) and induction of miR-27a–regulated ZBTB10, which has previously been characterized as an “Sp repressor.” The results show that the anticancer activity of WIN is due, in part, to PP2A-dependent disruption of miR-27a:ZBTB10 and ZBTB10-mediated repression of Sp transcription factors and Sp-regulated genes, including eIF4E. Mol Cancer Ther; 12(11); 2483–93. ©2013 AACR.
Early Dual Versus Mono Antiplatelet Therapy for Acute Non-Cardioembolic Ischemic Stroke or Transient Ischemic Attack
Authors: Wong Ka Sing Lawrence , Wang Yilong , Leng Xinyi , Mao Chen , Tang Jinling , Bath Philip M.W. , Markus Hugh S. , Gorelick Philip B. , Liu Liping , Lin Wenhua , Wang Yongjun 
Published: September 2013

Background—
Emerging studies suggest that early administration of dual antiplatelet therapy may be better than monotherapy for prevention of early recurrent stroke and cardiovascular outcomes in acute ischemic stroke and transient ischemic attack (TIA). We performed a meta-analysis of randomized, controlled trials evaluating dual versus mono antiplatelet therapy for acute noncardioembolic ischemic stroke or TIA.

Methods and Results—

We assessed randomized, controlled trials investigating dual versus mono antiplatelet therapy published up to November 2012 and the CHANCE trial (Clopidogrel in High-risk patients with Acute Non-disabling Cerebrovascular Events), for efficacy and safety outcomes in adult patients with acute noncardioembolic ischemic stroke or TIA with treatment initiated within 3 days of ictus. In total, 14 studies of 9012 patients were included in the systematic review and meta-analysis. Dual antiplatelet therapy significantly reduced risk of stroke recurrence (risk ratio, 0.69; 95% confidence interval, 0.60–0.80;
P
<0.001) and the composite outcome of stroke, TIA, acute coronary syndrome, and all death (risk ratio, 0.71; 95% confidence interval, 0.63–0.81;
P
<0.001) when compared with monotherapy, and nonsignificantly increased risk of major bleeding (risk ratio, 1.35; 95% confidence interval, 0.70–2.59,
P
=0.37). Analyses restricted to the CHANCE Trial or the 7 double-blind randomized, controlled trials showed similar results.

Conclusions—
For patients with acute noncardioembolic ischemic stroke or TIA, dual therapy was more effective than monotherapy in reducing risks of early recurrent stroke. The results of the CHANCE study are consistent with previous studies done in other parts of the world.
Influence of Chronic Hyperglycemia on Cerebral Microvascular Remodeling
Authors: Hou Qinghua , Zuo Zhiyi , Michel Patrik , Zhang Yanrong , Eskandari Ashraf , Man Fengyuan , Gao Qingcun , Johnston Karen C. , Wintermark Max 
Published: September 2013

Background and Purpose—
To investigate the effect of chronic hyperglycemia on cerebral microvascular remodeling using perfusion computed tomography.

Methods—

We retrospectively identified 26 patients from our registry of 2453 patients who underwent a perfusion computed tomographic study and had their hemoglobin A
1c
(HbA
1c
) measured. These 26 patients were divided into 2 groups: those with HbA
1c
>6.5% (n=15) and those with HbA
1c
≤6.5% (n=11). Perfusion computed tomographic studies were processed using a delay-corrected, deconvolution-based software. Perfusion computed tomographic values were compared between the 2 patient groups, including mean transit time, which relates to the cerebral capillary architecture and length.

Results—

Mean transit time values in the nonischemic cerebral hemisphere were significantly longer in the patients with HbA
1c
>6.5% (
P
=0.033), especially in the white matter (
P
=0.005). Significant correlation (
R
=0.469;
P
=0.016) between mean transit time and HbA
1c
level was observed.

Conclusions—
Our results from a small sample suggest that chronic hyperglycemia may be associated with cerebral microvascular remodeling in humans. Additional prospective studies with larger sample size are required to confirm this observation.
Γνωστικῶς and / or ὑλικῶς: Philoponus’ Account of the Material Aspects of Sense-Perception
Authors: Lautner Péter 
Published: September 2013

Abstract
The paper aims to show that Philoponus’ theory of sense-perception does not fit in with the spiritualist claim that the sensory process does not involve an extra material change in the sense-organ. Both the specific sense-organs (like the vitreous liquid and choroid or corneal membrane in the eyes) and the primary sense-organ (like the optic pneuma) contract or expand in the perceptual process. On the other hand, the literalist claim needs to be modified as well since only the tactile sense-organ (flesh) takes on the relevant qualities. Contraction or expansion in the sense-organ is triggered, not by physical changes in the medium, but by the formal activities arising from the perceptible objects: colours make the visual sense-organ contract or expand. At the level of sense-organs, the physiological process underlying sense-perception has three stages. The change in specific sense-organ will be transmitted to the primary sense-organ of the particular sense (optic/acoustic pneuma), and then reaches the common sense-organ, the pneuma. The primary sense-organs are spatially distinguishable parts of the common sense-organ which is otherwise homogeneous, not allowing for qualitative differences. The homogeneity of the pneuma establishes the unity of sense-perception at the level of physiological processes.
The Mystery of Ice Crystal Multiplication in a Laboratory Experiment
Authors: Santachiara Gianni , Belosi Franco , Prodi Franco 
Published: September 2013

Abstract
This paper addresses the problem of the large discrepancies between ice crystal concentrations in clouds and the number of ice nuclei in nearby clear air reported in published papers. Such discrepancies cannot always be explained, even by taking into account both primary and secondary ice formation processes. A laboratory experiment was performed in a cylindrical column placed in a cold room at atmospheric pressure and temperature in the −12° to −14°C range. Supercooled droplets were nucleated in the column, in the absence of aerosol ice nuclei, by injecting ice crystals generated outside in a small syringe. A rapid increase in the ice crystal concentration was observed in the absence of any known ice multiplication. The ratio between the mean number of ice crystals in the column, after complete droplet vaporization, and the number of ice crystals introduced in the column was about 10:1. The presence of small ice crystals (introduced at the top of the column) in the unstable system (supercooled droplets) appears to trigger the transformation in the whole supercooled liquid cloud. A possible explanation could be that the rapidly evaporating droplets cool sufficiently to determine a homogeneous nucleation.