bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anticancer activity of Araguspongine C via inducing apoptosis, and inhibition of oxidative stress, inflammation, and EGFR‐TK in human lung cancer cells: An in vitro and in vivo study
Authors: Li Zhe , Yu Hongjiang , Hussain Shaik Althaf , Yang Rui 
Published: July 2024

AbstractThe advanced non‐small cell lung cancer (NSCLC) that harbors epidermal growth factor receptor (EGFR) mutations has put a selective pressure on the discovery and development of newer EGFR inhibitors. Therefore, the present study intends to explore the pharmacological effect of Araguspongine C (Aragus‐C) as anticancer agent against lung cancer. The effect of Aragus‐C was evaluated on the viability of the A549 and H1975 cells. Further biochemical assays were performed to elaborate the effect of Aragus‐C, on the apoptosis, cell‐cycle analysis, and mitochondrial membrane potential in A549 cells. Western blot analysis was also conducted to determine the expression of EGFR in A549 cells. Tumor xenograft mice model from A549 cells was established to further elaborate the pharmacological activity of Aragus‐C. Results suggest that Aragus C showed significant inhibitory activity against A549 cells as compared to H1975 cells. It has been found that Aragus‐C causes the induction of apoptosis and promotes cell‐cycle arrest at the G2/M phase of A549 cells. It also showed a reduction in the overexpression of EGFR in A549 cells. In tumor xenograft mice model, it showed a significant reduction of tumor volume in a dose‐dependent manner, with maximum inhibitory activity was reported by the 8 mg/kg treated group. It also showed significant anti‐inflammatory and antioxidant activity by reducing the level of TNF‐α, IL‐1β, IL‐6, and MDA, with a simultaneous increase of superoxide dismutase and glutathione peroxidase. We have demonstrated the potent anti‐lung cancer activity of Aragus‐C, and it may be considered as a potential therapeutic choice for NSCLC treatment.
An exploration of counselors of color working in the eating disorder field
Authors: Biang Amy , Merlin‐Knoblich Clare , Lim Jae Hoon 
Published: July 2024

AbstractAmong eating disorder (ED) professionals, counselors of color (COCs) are underrepresented. Given the prevalence of EDs among people of all racial and ethnic backgrounds, as well as the low number of COCs working in the profession and its problematic implications, this study is warranted to include the experiences of COCs in EDs and understand how to grow the specialty with counselors of all backgrounds. In this study, we interviewed 10 COCs and analyzed transcripts using a post‐intentional phenomenological design. Tentative manifestations of unprepared, isolating spaces, unspoken knowing, and exhaustion, as well as provocations of cultural inclusion, changemaker, and vulnerability, emerged. We present implications, limitations, and research considerations.
mTORC2: A neglected player in aging regulation
Authors: Xu Weitong , Chen Honghan , Xiao Hengyi 
Published: July 2024

AbstractMammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a pivotal role in various biological processes, through integrating external and internal signals, facilitating gene transcription and protein translation, as well as by regulating mitochondria and autophagy functions. mTOR kinase operates within two distinct protein complexes known as mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2), which engage separate downstream signaling pathways impacting diverse cellular processes. Although mTORC1 has been extensively studied as a pro‐proliferative factor and a pro‐aging hub if activated aberrantly, mTORC2 received less attention, particularly regarding its implication in aging regulation. However, recent studies brought increasing evidence or clues for us, which implies the associations of mTORC2 with aging, as the genetic elimination of unique subunits of mTORC2, such as RICTOR, has been shown to alleviate aging progression in comparison to mTORC1 inhibition. In this review, we first summarized the basic characteristics of mTORC2, including its protein architecture and signaling network. We then focused on reviewing the molecular signaling regulation of mTORC2 in cellular senescence and organismal aging, and proposed the multifaceted regulatory characteristics under senescent and nonsenescent contexts. Next, we outlined the research progress of mTOR inhibitors in the field of antiaging and discussed future prospects and challenges. It is our pleasure if this review article could provide meaningful information for our readers and call forth more investigations working on this topic.
Neuromedin S regulates goat ovarian granulosa cell proliferation and steroidogenesis via endoplasmic reticulum Ca2+‐YAP1‐ATF4‐c‐Jun pathway
Authors: Sun Xuan , Xia Rongxin , Wang Yifei , Wang Feng , Liu Zhipeng , Xue Gang , Zhang Guomin 
Published: July 2024

AbstractNeuromedin S (NMS) plays key roles in reproductive regulation, while its function and mechanism in follicular development remain unclear. The current study aims to investigate the specific role and mechanisms of NMS and its receptors in regulating the proliferation and steroidogenesis of ovarian granulosa cells (GCs). Phenotypically, a certain concentration of NMS addition promoted the proliferation and estrogen production of goat GCs, accompanied by an increase in the G1/S cell population and upregulation of the expression levels of cyclin D1, cyclin dependent kinase 6, steroidogenic acute regulatory protein, cytochrome P450, family 11, subfamily A, polypeptide 1, 3beta‐hydroxysteroid dehydrogenase, and cytochrome P450, family 11, subfamily A, polypeptide 1, while the effects of NMS treatment were effectively hindered by knockdown of neuromedin U receptor type 2 (NMUR2). Mechanistically, activation of NMUR2 with NMS maintained endoplasmic reticulum (ER) calcium (Ca2+) homeostasis by triggering the PLCG1‐IP3R pathway, which helped preserve ER morphology, sustained an appropriate level of endoplasmic reticulum unfolded protein response (UPRer), and suppressed the nuclear translocation of activating transcription factor 4. Moreover, NMS maintained intracellular Ca2+ homeostasis to activate the calmodulin 1‐large tumor suppressor kinase 1 pathway, ultimately orchestrating the regulation of goat GC proliferation and estrogen production through the Yes1 associated transcriptional regulator‐ATF4‐c‐Jun pathway. Crucially, the effects of NMS were mitigated by concurrent knockdown of the NMUR2 gene. Collectively, these data suggest that activation of NMUR2 by NMS enhances cell proliferation and estrogen production in goat GCs through modulating the ER and intracellular Ca2+ homeostasis, leading to activation of the YAP1‐ATF4‐c‐Jun pathway. These findings offer valuable insights into the regulatory mechanisms involved in follicular growth and development, providing a novel perspective for future research.
Curcumin‐activated Wnt5a pathway mediates Ca2+ channel opening to affect myoblast differentiation and skeletal muscle regeneration
Authors: Wang Mao‐yuan , Yang Jia‐ming , Wu Yi , Li Hai , Zhong Yan‐biao , Luo Yun , Xie Rui‐lian 
Published: July 2024

AbstractBackgroundSkeletal muscle injury is one of the most common sports injuries; if not properly treated or not effective rehabilitation treatment after injury, it can be transformed into chronic cumulative injury. Curcumin, an herbal ingredient, has been found to promote skeletal muscle injury repair and regeneration. The Wnt5a pathway is related to the expression of myogenic regulatory factors, and Ca2+ promotes the differentiation and fusion process of myoblasts. This study explored the effect and mechanism of curcumin on myoblast differentiation during the repair and regeneration of injured skeletal muscle and its relationship with the Wnt5a pathway and Ca2+ channel.MethodsMyogenic differentiation of C2C12 cells was induced with 2% horse serum, and a mouse (male, 10 weeks old) model of acute skeletal muscle injury was established using cardiotoxin (20 μL). In addition, we constructed a Wnt5a knockdown C2C12 cell model and a Wnt5a knockout mouse model. Besides, curcumin was added to the cell culture solution (80 mg/L) and fed to the mice (50 mg/kg). Fluorescence microscopy was used to determine the concentration of Ca2+. Western blot and RT‐qPCR were used to detect the protein and mRNA levels of Wnt5a, CaN, NFAT2, MyoD, Myf5, Pax7, and Myogenin. The expression levels of MyoD, Myf5, Myogenin, MHC, Desmin, and NFAT2 were detected using immunofluorescence techniques. In addition, MyoD expression was observed using immunohistochemistry, and morphological changes in mouse muscle tissue were observed using HE staining.ResultsDuring myoblast differentiation and muscle regeneration, Wnt5a expression was upregulated (P < 0.001) and the Wnt5a signalling pathway was activated. Wnt5a overexpression promoted the expression of MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.05), and conversely, knockdown of Wnt5a inhibited their expression (P < 0.001). The Wnt5a pathway mediated the opening of Ca2+ channels, regulated the expression levels of CaN, NFAT2, MyoD, Myf5, Myogenin, MHC, and Desmin (P < 0.01) and promoted the differentiation of C2C12 myoblasts and the repair and regeneration of injured skeletal muscle. The expression of Wnt5a, CaN, NFAT2, MyoD, Myogenin, Myf5, and MHC in C2C12 myoblast was significantly increased after curcumin intervention (P < 0.05); however, their expression decreased significantly after knocking down Wnt5a on the basis of curcumin intervention (P < 0.05). Similarly, in Wnt5a knockout mice, the promotion of muscle regeneration by curcumin was significantly attenuated.ConclusionsCurcumin can activate the Wnt5a signalling pathway and mediate the opening of Ca2+ channels to accelerate the myogenic differentiation of C2C12 cells and the repair and regeneration of injured skeletal muscle.
Methacrylonitrile‐based adsorbents for recovery of VFAs from fermentation broth
Authors: Elhami Vahideh , Ronka Sylwia , Schuur Boelo 
Published: July 2024

AbstractBACKGROUNDAdsorption is a promising affinity separation technique to target a sorbate in extremely dilute solutions. In this work, methacrylonitrile (MAN)‐functionalized resins were investigated to recover volatile fatty acids (VFAs) from a mimicked fermentation broth as an alternative for the already known amine‐based resins and non‐functionalized poly(styrene‐divinylbenzene) (PS‐DVB), aiming for high VFA capacity and selectivity.RESULTSNext to comparison with commercial PS‐DVB resin, also several PS‐DVB resins were synthesized as non‐functionalized analogues for the new MAN‐functionalized resins. For MAN‐functionalized resins, the maximum equilibrium VFA loading of about 153 (g acid/kg adsorbent) was found, which is higher than the 125 (g acid/kg adsorbent) for the PS‐DVB non‐functionalized resins. The water uptake of the PS‐DVB resins was with 0.53–0.87 (g water/g adsorbent) significantly lower than for the MAN‐DVB resins taking up 0.99–1.85 (g water/g adsorbent).CONCLUSIONOverall, the MAN‐functionalized adsorbents retained higher loading capacity for corresponding acid, compared to the PS‐DVB resins, while also taking in more water. Acid uptake is due to the hydrogen bonding between the nitrile groups of the resins with carboxyl group of the acids. Moreover, none of the adsorbents displayed an affinity towards the salts present in the fermentation broth. © 2024 The Author(s). Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Novel base‐catalyzed acylation of benzenediols with vinyl acetate: a Hirshfeld surface study
Authors: Ali Imran , Isaev Alexey Yu , Chichigina Yana M , Nakov Daniil D , Shigabaeva Gulnara N , Galunin Evgeny V , Locatelli Marcello , Almarhoon Zainab M 
Published: July 2024

AbstractBACKGROUNDThe present work describes the facile, fast and economic base‐catalyzed acylation of benzenediols with vinyl acetate. The Hirshfeld surface analysis of benzene‐1,4‐diol O,O′‐diacetate is also described. NaOH, KOH and K2CO3 were the bases used for the catalysis of the acylation of benzene‐1,x‐diols (x = 2, 3, 4) with vinyl acetate to obtain the corresponding O,O′‐diacetyl derivatives.RESULTSThe yields were quite good, up to 99% under solvent‐free conditions. This method represents an alternative ‘green’ process for the preparation of O‐acetyl derivatives of hydroxyaryls. The benzene‐1,4‐diol O,O′‐diacetate crystal structure was obtained and described using Hirshfeld surface analysis. A noticeable contribution of the C⋯H contacts is shown (16.7%), despite the insignificant area of the Hirshfeld surface occupied by the carbon atoms (9.4%). By using two‐dimensional fingerprint plots, it was proved that C⋯H contacts were due to the presence of π‐stacking. The O⋯H contacts (41.4%) were the most numerous ones, relating mainly to typical hydrogen bonds (de + di ≈ 2.6 Å). The high saturation of the C⋯H and O⋯H contacts (enrichment ratios (E) = 1.30 and 1.36, respectively), despite their smaller contribution to the overall surface as compared to the H⋯H contacts (E = 0.84), is shown.CONCLUSIONThe novelty of this work is the development of a method based on an inexpensive, safe and easy‐to‐use catalyst (K2CO3). This method is fast with high yields. Besides, it may be used for acylation at an industrial scale. © 2024 Society of Chemical Industry (SCI).
Dental students’ Myers‐Briggs Type Indicator personality profile in the past 50 years: Systematic review and meta‐analysis
Authors: Li Chenshuang , Azami Niloufar , Campos Hugo , Chan Michael , Van Anne Doan , Tisot Pamela , Goolsby Susie 
Published: July 2024

AbstractBackgroundDental students’ personalities are strongly correlated with their didactic and clinical performances. With the significant changes in the social environment in the past decades, students’ personalities may also change dramatically. Additionally, with the increasing number of international students admitted into US dental programs, educators must pay attention to the potential personality differences between domestic and international students.BackgroundA systematic review focusing on the Myers‐Briggs Type Indicator personality types of dental students was conducted with 11 literature databases following the Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guideline. The quality assessment of each included article was conducted following the Joanna Briggs Institute Prevalence Critical Appraisal Tool. Meta‐analyses were conducted for each personality type within the United States in the past 50 years, and in each country in the past 20 years.ResultsEighteen articles (17 reports) were included after the eligibility assessment. The longitudinal trends within the United States showed the predominant two personality types have changed from Extraversion, Sensing, Feeling, and Judging (ESFJ) and Extraversion, Sensing, Thinking, and Judging (ESTJ) to ESTJ and Introversion, Sensing, Thinking, and Judging (ISTJ) during the past 50 years. When comparing different countries, the United States, China, Korea, and Iran have the same two dominant personality types (ESTJ and ISTJ) but not the Philippines (Extraversion, Intuition, Feeling, and Perceiving and Introversion, Intuition, Feeling, and Perceiving). However, there are large variations in the prevalence of other personality types.ConclusionThere have been constant changes in the predominant personalities of dental students over the years. Additionally, understanding the diversity of personality types within the United States as well as among different countries could serve as the foundation for further improvements in teaching strategies and student support services.
Rose bengal photocatalyzed expeditious multicomponent synthesis of 3,4‐dihydropyrimidin‐2‐(1H)‐ones/thiones under visible light irradiation
Authors: Patel Divyani P. , Patel Vishwa K. , Singh Satish Kumar 
Published: July 2024

AbstractThe combination of visible light, a renewable and green energy source, and rose bengal, a non‐toxic organic dye as a photoredox catalyst, is an easy and efficient method for synthesizing 3,4‐dihydropyrimidin‐2(1H)‐ones/thiones in EtOH at ambient temperature in very short reaction time. The present work offers simple operation, easy workup, rapid conversion, and excellent product yields, while accommodating a wide range of substrates. Rose bengal‐based photocatalytic approach permits foremost sustainability, which delivers economic and environmental rewards.
Applying the Effective Programme Coverage framework to assess gaps in HIV prevention programmes for female sex workers and men who have sex with men in Nairobi, Kenya: findings from an expanded Polling Booth Survey
Authors: Bhattacharjee Parinita , McClarty Leigh , Isac Shajy , Kimani Joshua , Emmanuel Faran , Kabuti Rhoda , Kinyua Antony , Kombo Bernadette Kina , Owek Collins , Musyoki Helgar , Kiplagat Anthony , Arimi Peter , Shaw Souradet Yuh‐Nan , Gandhi Monica , Malone Siobhan , Blanchard James , Garnett Geoff , Becker Marissa L. 
Published: July 2024

AbstractIntroductionMeasuring the coverage of HIV prevention services for key populations (KPs) has consistently been a challenge for national HIV programmes. The current frameworks and measurement methods lack emphasis on effective coverage, occur infrequently, lack timeliness and limit the participation of KPs. The Effective Programme Coverage framework, which utilizes a programme science approach, provides an opportunity to assess gaps in various coverage domains and explore the underlying reasons for these gaps, in order to develop targeted solutions. We have demonstrated the application of this framework in partnership with the KP community in Nairobi, Kenya, using an expanded Polling Booth Survey (ePBS) method.MethodsData were collected between April and May 2023 among female sex workers (FSWs) and men who have sex with men (MSM) using (a) PBS, (b) bio‐behavioural survey and (c) focus group discussions. Data collection and analysis involved both KP community and non‐community researchers. Descriptive analysis was performed, and proportions were used to assess the programme coverage gaps. The data were weighted to account for the sampling design and unequal selection probabilities. Thematic analysis was conducted on the qualitative data.ResultsThe condom programme for FSW and MSM had low availability (60.2% and 50.9%), contact (68.8% and 65.9%) and utilization (52.1% and 43.9%) coverages. The pre‐exposure prophylaxis (PrEP) programme had very low utilization coverage for FSW and MSM (4.4% and 2.8%), while antiretroviral therapy utilization coverage was higher (86.6% and 87.7%). Reasons for coverage gaps included a low peer educator‐to‐peer ratio, longer distance to the clinics, shortage of free condoms supplied by the government, experienced and anticipated side effects related to PrEP, and stigma and discrimination experienced in the facilities.ConclusionsThe Effective Programme Coverage framework allows programmes to assess coverage gaps and develop solutions and a research agenda targeted at specific domains of coverage with large gaps. The ePBS method works well in collecting data to understand coverage gaps rapidly and allows for the engagement of the KP community.
ChemsexPH: The association between chemsex, HIV status and adherence to antiretroviral therapy among men who have sex with men in the Philippines
Authors: Olete Rodenie Arnaiz , Strong Carol , Leyritana Katerina , Bourne Adam , Ko Nai‐Ying Monica 
Published: July 2024

AbstractIntroductionChemsex, the use of psychotropic drugs before or during sexual intercourse, is associated with various HIV risk factors, including condomless sex and reduced adherence to pre‐exposure prophylaxis or antiretroviral therapy (ART). In the Philippines, there are still limited studies exploring the associations between chemsex, HIV status and ART adherence. This study aims to compare recent and lifetime chemsex engagement in association with self‐reported HIV status among Filipino men who have sex with men (MSM). We further explored the association between chemsex and ART adherence among people living with HIV engaged in chemsex.MethodsA cross‐sectional online survey of 479 Filipino MSM was conducted from 3 August to 1 December 2019. Demographic profiles, sexual behaviours, drug use, history of sexually transmitted infections (STIs), chemsex engagement and HIV status were collected and analysed. Bivariable and multivariable logistic regression were employed to assess the association between self‐reported HIV status and chemsex engagement.ResultsAmong the 479 respondents, Filipino MSM engaged in drug use and chemsex were generally older compared to those not engaged in drug use and chemsex (average age 31−33 vs. 29 years old; p<0.05). Methamphetamine was the most common drug for people who reported using drugs. An HIV‐positive status was associated with recent chemsex engagement (aOR = 5.18, p<0.05) and a history of STIs (aOR = 2.09, p<0.05). The subgroup analysis showed that 79% (166/200) of persons living with HIV were adherent to ART. There was no significant association found between chemsex and ART adherence in the logistic regression analyses.ConclusionsChemsex behaviour, particularly recent chemsex engagement, is significantly associated with self‐reported HIV status. The emerging data on MSM engaged in chemsex require integration of a more person‐centred, comprehensive and robust harm reduction programmes into the existing combination prevention strategies in the country. Health education for Filipino MSM engaged in chemsex should prioritize raising awareness about methamphetamine effects and overdose risks, alongside proper medical management.
Enhanced resistance of Lactiplantibacillus plantarum by expression of albumin
Authors: Ding Tongren , Wang Guangqiang , Tang Liuqian , Xia Yongjun , Song Xin , Yang Yijin , Ai Lianzhong 
Published: July 2024

AbstractBACKGROUNDHuman serum albumin (HSA) is the most abundant protein in plasma, playing crucial roles in regulating osmotic pressure and maintaining protein homeostasis. It is widely applied in the clinical treatment of various diseases. HSA can be purified from plasma or produced using recombinant DNA technology. Due to the improved efficiency and reduced costs, a growing body of research has focused on enhancing albumin production through bacterial strain overexpression. However, there have been few studies on the effect of albumin on the characteristics of the overexpressing‐strain itself, particularly stress resistance. In this study, we utilized Lactiplantibacillus plantarum (L. plantarum) AR113 as the expression host and successfully constructed the albumin overexpression strain AR113‐pLLY01 through gene editing technology. The successful expression of albumin was achieved and subsequently compared with the wild‐type strain AR113‐pIB184.RESULTSThe results demonstrated that the survival rate of AR113‐pLLY01 was also significantly better than that of AR113‐pIB184 after lyophilization. In addition, AR113‐pLLY01 exhibited a significantly better protective effect than AR113‐pIB184 at pH 3, indicating that albumin possesses a certain tolerance to acidic stress. At bile salt concentrations higher than 0.03%, both strains showed limited growth, but at a concentration of 0.02%, AR113‐pLLY01 had a significant protective effect.CONCLUSIONThis study suggest that albumin can improve strain tolerance, which has significant implications for future applications. © 2024 Society of Chemical Industry.