bluuebunny/crossref_metadata_2025_split huggingface dataset:
Study of synergistic behavior during bituminous coal-cow manure co-gasification: The role of intrinsic AAEM and organic matter
Authors: Lu Hongqiao , Ma Meng , Wei Juntao , Bai Yonghui , Lv Peng , Wang Jiaofei , Song Xudong , Lu Guanghua , Yu Guangsuo 
Published: July 2024

AbstractCo-thermal chemical conversion of coal and biomass is one of the important ways to realize efficient and clean utilization of coal. In this study, a typical Ningdong coal-Yangchangwan bituminous coal and cow manure were used to study the synergistic effect of intrinsic alkali, alkaline earth metals (AAEM) and organic matter on the co-gasification of coal and biomass by thermogravimetry analyzer (TG). The results showed that AAEM had obvious synergistic promotion effect on the gasification of a bituminous coal-cow manure mixture in the isothermal gasification (1000 ℃), whereas the organic matter will show the opposite effect on the process. To further investigate the effect of organic matter on the gasification process, the influence of organic matter on non-isothermal (25-1000 ℃) gasification reaction was investigated with heating rate of 10 ℃ /min, the kinetic parameters of the gasification reaction were obtained by Coats-Redfern method. The increase of biomass mass fraction in the sample facilitates the migration of alkali metals from the material to the solid phase. The possible mechanism of the synergistic effect of intrinsic AAEM/organic matter on the co-gasification process was proposed.
Feature learning and generalization error analysis of two-layer linear neural networks for high-dimensional inputs
Authors: Nishimori Hayato , Suzuki Taiji 
Published: July 2024

AbstractIt is well known that a model can generalize even when it completely interpolates the training data, which is known as the benign overfitting. Indeed, several work have theoretically revealed that the minimum-norm interpolator can exhibit the benign overfitting. On the other hand, deep learning models such as two-layer neural networks have been reported to outperform “shallow” learning models such as kernel methods under appropriate model sizes by adaptively learning the basis functions to the data. This mechanism is called feature learning, and it is known empirically to be beneficial even when the model size is large. However, it is generally difficult to show that benign overfitting occurs in learning models with feature learning especially for regression problems. In this study, we then analyze the predictive error of the estimator after one step feature learning in a two-layer linear neural network optimized by gradient descent methods and study the effect of feature learning on benign overfitting. The results show that feature learning reduces bias compared to a one-layer linear regression model without feature learning, especially when the eigenvalues of the covariance of input decay slowly. On the other hand, we clarify that the variance is hardly changed by feature learning. This differs significantly from the results for benign overfitting in the situation without feature learning and indicates the usefulness of feature learning.
Identifying and optimization of critical process parameters for the modulation of polysaccharide molecular size in Streptococcus pneumoniae serotype-1
Authors: Sangareddy Veerapandu , Mallu Maheswara Reddy , Matur Ramesh V. , Shaik Fayaz Basha , Nettem Balaprasad , Gajavelli Srujan 
Published: July 2024

AbstractMaintaining the molecular size (MS) of Streptococcus pneumoniae capsular polysaccharide within specified range is essential for manufacture of conjugate vaccines, either through physical or acid hydrolysis before use in the conjugation process. Polysaccharide MS typically reduced, with high-pressure homogenization as an approach, for preserving their chemical structure. When the average MS of Pneumococcal capsular polysaccharide serotype-1(CPS1) exceeds 1200 kDa during fermentation, using a high-pressure homogenizer to reduce its MS to 150–250 kDa can become extremely difficult. Even after multiple homogenization cycles, obtaining polysaccharide of the required size can be challenging. Moreover, exceeding a certain number of homogenization cycles can negatively impact the stability, yield, and conjugation efficiency. To control polysaccharide MS, we conducted a design of experiments (DOE) study focused on the optimization of the fermentation process, employing serotype-1 as a represntative case. The successful optimization of these CPPs was achieved in a consistent and reproducible manner. Systematic evaluation by DOE based process optimization has provided valuable insights into precise polysaccharide manufacturing control of polysaccharide MS. Our findings confirm that maintaining Hy-Soy™ at 20-30 g/L and yeast extract at 1–3 g/L in the fermentation media, with a feed concentration of 2–3.5 g/L/H and 0.1–0.5 VVM of air, consistently yields polysaccharide with MS of < 1200 kDa. This strategy that can be extended to other S. pneumoniae serotype polysaccharide production.
Graphical abstract
Measles Outbreak Investigation in Aneded District, Northwest Ethiopia: A Case-Control Study
Authors: Tariku Mengistie Kassahun , Belete Abebe Habtamu , Worede Daniel Tarekegn , Tegegne Bantayehu Addis , Bante Simachew Animen , Misikir Sewnet Wongiel 
Published: August 2024

AbstractBackgroundBetween 2000 and 2018, global measles deaths decreased by 73%, but the disease remains prevalent in many developing countries, especially in Africa and Asia. Although Ethiopia was attempting to eliminate the measles, it still ranks fourth in the world in terms of the number of cases. The aim of the investigation was to describe the outbreak and identify its determinants in the Aneded district.MethodsBetween March 3, 2020, and April 2, 2020, the 89 patients and 178 controls participated in a case-control study. Data were gathered by means of in-person interviews with household leaders. The attack and case fatality rates were determined. In multivariable logistic regression analysis, variables having a p-value of less than 0.05 were considered statistically significant cut-off points.ResultsAn investigation was conducted on a total of 89 measles cases, with 3 deaths and 178 controls. In total, there were 1.65 attacks per 1000 people, or 3.4% of the case fatality rate. There were 155 days of outbreak duration. The disease was significantly associated with being female [adjusted odds ratios (AOR) = 2.66; 95% confidence interval (CI) = 1.38–5.11], under 5 years old [AOR = 7.24; 95% CI = 2.58–20.31], positive in attitude [AOR = 0.22; 95% CI = 0.11–0.42], and having a contact history [AOR = 3.19; 95% CI = 1.67–6.10].ConclusionThe measles outbreak, with its higher attack and case fatality rate, has been influenced by factors like household attitudes, age, sex, contact and travel history and needs to be reduced through early detection, active surveillance, and fostering favorable attitudes towards disease prevention and control.
Rain‐Induced Surface Sensible Heat Flux Reduces Monsoonal Rainfall Over India
Authors: Zhou Xin , Ray Pallav , Tan Haochen , Dudhia Jimy , Ajayamohan R. S. , Gomes Helber , Pan Yipeng 
Published: July 2024

AbstractPrecipitation can induce a surface sensible heat flux since the raindrops are generally cooler than the surface. This precipitation‐induced sensible heat flux (QP) is typically ignored in models. However, during heavy rainfall, QP can be large and may not be negligible such as over India during the summer monsoon season. We provide the first results of incorporating QP in a simulation that shows ∼2% (∼5%) reduction in precipitation over India compared to the simulation without QP during a monsoonal active phase in 2017 (2018). This reduction was primarily due to a reduction in vertical advection of moisture. Additionally, QP modified the spatial distribution of precipitation with 40% of the geographical area encountering alterations of at least 20% in precipitation. This change in precipitation distribution across the region can have important implications for regional agriculture and irrigation practices. Changes in the partitioning of surface heat flux components due to QP is also discussed.
Using umbilical cord tissue to identify prenatal ethanol exposure and co-exposure to other commonly misused substances
Authors: Jones Joseph , Coy Donna , Gidron Dominique , Hariharan Shanthi , Jones Mary , Patel Niranjan , Racines Amy , Toma Sarah , Brown Guida 
Published: July 2024

Abstract
Objective
Substance misuse during pregnancy can result in a variety of poor pregnancy outcomes. Objective data reporting the prevalence of neonates born with ethanol metabolites (evidence of prenatal ethanol exposure) in their fluids or tissues are limited.

Study design
A secondary analysis of umbilical cord tissue specimens received for routine toxicological analysis was conducted. Prevalences of ethyl glucuronide (EtG), a long-term direct ethanol biomarker, were determined using a new laboratory tool, LDTD-MSMS. Additionally, other commonly misused substances were determined using routine procedures.

Results
Of 12,995 specimens, 238 (1.8%) specimens contained EtG. Concentrations of EtG ranged from 5 ng/g to 6679 ng/g (median 47 ng/mg; IQR: 16 ng/g, 203 ng/g). Of those 238 EtG-positive specimens, nearly 58% (N = 138) contained additional substances or metabolites.

Conclusion
Self-report of substance use during pregnancy is under-reported. We have demonstrated co-exposure of substances with ethanol is higher than previous reports.
Peroxisomal cholesterol metabolism regulates yap-signaling, which maintains intestinal epithelial barrier function and is altered in Crohn’s disease
Authors: Pinelli Marinella , Makdissi Stephanie , Scur Michal , Parsons Brendon D. , Baker Kristi , Otley Anthony , MacIntyre Brad , Nguyen Huong D. , Kim Peter K. , Stadnyk Andrew W. , Di Cara Francesca 
Published: July 2024

AbstractIntestinal epithelial cells line the luminal surface to establish the intestinal barrier, where the cells play essential roles in the digestion of food, absorption of nutrients and water, protection from microbial infections, and maintaining symbiotic interactions with the commensal microbial populations. Maintaining and coordinating all these functions requires tight regulatory signaling, which is essential for intestinal homeostasis and organismal health. Dysfunction of intestinal epithelial cells, indeed, is linked to gastrointestinal disorders such as irritable bowel syndrome, inflammatory bowel disease, and gluten-related enteropathies. Emerging evidence suggests that peroxisome metabolic functions are crucial in maintaining intestinal epithelial cell functions and intestinal epithelium regeneration and, therefore, homeostasis. Here, we investigated the molecular mechanisms by which peroxisome metabolism impacts enteric health using the fruit fly Drosophila melanogaster and murine model organisms and clinical samples. We show that peroxisomes control cellular cholesterol, which in turn regulates the conserved yes-associated protein-signaling and contributes to intestinal epithelial structure and epithelial barrier function. Moreover, analysis of intestinal organoid cultures derived from biopsies of patients affected by Crohn’s Disease revealed that the dysregulation of peroxisome number, excessive cellular cholesterol, and inhibition of Yap-signaling are markers of disease and could be novel diagnostic and/or therapeutic targets for treating Crohn’s Disease. Our studies provided mechanistic insights on peroxisomal signaling in intestinal epithelial cell functions and identified cholesterol as a novel metabolic regulator of yes-associated protein-signaling in tissue homeostasis.
EZH2–STAT3 signaling pathway regulates GSDMD-mediated pyroptosis in glioblastoma
Authors: Yu Dong , Wang Shuai , Wang Jiajun , Zhang Kang , Niu Zihui , Lin Ning 
Published: July 2024

AbstractGlioblastoma multiforme (GBM) is the most therapeutically challenging primary brain tumor owing to the unique physiological structure of the blood–brain barrier. Lately, research on targeted therapy for gliomas has shifted focus toward the tumor microenvironment and local immune responses. Pyroptosis is a newly identified cellular demise characterized by the release of numerous inflammatory factors. While pyroptosis shows promise in impeding the occurrence and progression of GBM, the regulatory mechanisms governing this process in gliomas still require further investigation. The function of the Enhancer of zeste homolog 2 (EZH2) in pyroptosis remains unexplored. In this study, we discovered that 3-Deazaneplanocin A (DZNep), an inhibitor of EZH2, can induce pyroptosis in GBM in vitro experiments. Moreover, our investigation unveiled that the signal transducer and activator of transcription (STAT3) could serve as a downstream regulator influenced by EZH2, impacting pyroptosis in GBM. Following treatment with DZNep and the STAT3 inhibitor (SH-4–54), there was an elevation in the levels of pyroptosis-related factors, namely NOD-like receptor thermal protein domain-associated protein 3 (NLRP3) and Gasdermin D (GSDMD). Moreover, simultaneous inhibition of both EZH2 and STAT3 led to the expression of inflammatory factors such as IL-1β and IL-18. In summary, we have identified that EZH2 regulates pyroptosis in GBM through STAT3, and pyroptosis could potentially be targeted for immunotherapy in GBM.
Preclinical evaluation of tissue-selective gene therapies for congenital generalised lipodystrophy
Authors: Tiwari Mansi , Roumane Ahlima , Sommer Nadine , Han Weiping , Delibegović Mirela , Rochford Justin J. , Mcilroy George D. 
Published: July 2024

AbstractLipodystrophy is a rare disorder which can be life-threatening. Here individuals fail to develop or maintain appropriate adipose tissue stores. This typically causes severe metabolic complications, including hepatic steatosis and lipoatrophic diabetes. There is no cure for lipodystrophy, and treatment options remain very limited. Here we evaluate whether tissue-selective adeno-associated virus (AAV) vectors can provide a targeted form of gene therapy for lipodystrophy, using a preclinical lipodystrophic mouse model of Bscl2 deficiency. We designed AAV vectors containing the mini/aP2 or thyroxine-binding globulin promoter to selectively target adipose or liver respectively. The AAV-aP2 vectors also contained the liver-specific microRNA-122 target sequence, restricting hepatic transgene expression. Systemic delivery of AAV-aP2 vectors overexpressing human BSCL2 restored adipose tissue development and metabolic health in lipodystrophic mice without detectable expression in the liver. High doses (1 × 1012 GCs) of liver-selective vectors led to off target expression and adipose tissue development, whilst low doses (1 × 1010 GCs) expressed selectively and robustly in the liver but did not improve metabolic health. This reveals that adipose tissue-selective, but not liver directed, AAV-mediated gene therapy is sufficient to substantially recover metabolic health in generalised lipodystrophy. This provides an exciting potential new avenue for an effective, targeted, and thereby safer therapeutic intervention.
Optimization of fluorinated phenyl azides as universal photocrosslinkers for semiconducting polymers
Authors: Tan Zhao-Siu , Jamal Zaini , Teo Desmond W. Y. , Ko Hor-Cheng , Seah Zong-Long , Phua Hao-Yu , Ho Peter K. H. , Png Rui-Qi , Chua Lay-Lay 
Published: July 2024

AbstractFluorinated phenyl azides (FPA) enable photo-structuring of π-conjugated polymer films for electronic device applications. Despite their potential, FPAs have faced limitations regarding their crosslinking efficiency, and more importantly, their impact on critical semiconductor properties, such as charge-carrier mobility. Here, we report that azide photolysis and photocrosslinking can achieve unity quantum efficiencies for specific FPAs. This suggests preferential nitrene insertion into unactivated C‒H bonds over benzazirine and ketenimine reactions, which we attribute to rapid interconversion between the initially formed hot states. Furthermore, we establish a structure‒activity relationship for carrier mobility quenching. The binding affinity of FPA crosslinker to polymer π-stacks governs its propensity for mobility quenching in both PM6 and PBDB-T used as model conjugated polymers. This binding affinity can be suppressed by FPA ring substitution, but varies in a non-trivial way with π-stack order. Utilizing the optimal FPA, photocrosslinking enables the fabrication of morphology-stabilized, acceptor-infiltrated donor polymer networks (that is, PBDB-T: ITIC and PM6: Y6) for solar cells. Our findings demonstrate the exceptional potential of the FPA photochemistry and offer a promising approach to address the challenges of modelling realistic molecular interactions in complex polymer morphologies, moving beyond the limitations of Flory‒Huggins mean field theory.
Confinement of excited states in two-dimensional, in-plane, quantum heterostructures
Authors: Kim Gwangwoo , Huet Benjamin , Stevens Christopher E. , Jo Kiyoung , Tsai Jeng-Yuan , Bachu Saiphaneendra , Leger Meghan , Song Seunguk , Rahaman Mahfujur , Ma Kyung Yeol , Glavin Nicholas R. , Shin Hyeon Suk , Alem Nasim , Yan Qimin , Hendrickson Joshua R. , Redwing Joan M. , Jariwala Deep 
Published: July 2024

AbstractTwo-dimensional (2D) semiconductors are promising candidates for optoelectronic application and quantum information processes due to their inherent out-of-plane 2D confinement. In addition, they offer the possibility of achieving low-dimensional in-plane exciton confinement, similar to zero-dimensional quantum dots, with intriguing optical and electronic properties via strain or composition engineering. However, realizing such laterally confined 2D monolayers and systematically controlling size-dependent optical properties remain significant challenges. Here, we report the observation of lateral confinement of excitons in epitaxially grown in-plane MoSe2 quantum dots (~15-60 nm wide) inside a continuous matrix of WSe2 monolayer film via a sequential epitaxial growth process. Various optical spectroscopy techniques reveal the size-dependent exciton confinement in the MoSe2 monolayer quantum dots with exciton blue shift (12-40 meV) at a low temperature as compared to continuous monolayer MoSe2. Finally, single-photon emission (g2(0) ~ 0.4) was also observed from the smallest dots at 1.6 K. Our study opens the door to compositionally engineered, tunable, in-plane quantum light sources in 2D semiconductors.
Large scale exploration reveals rare taxa crucially shape microbial assembly in alkaline lake sediments
Authors: Qiu Zhiguang , He Shuhang , Lian Chun-Ang , Qiao Xuejiao , Zhang Qing , Yao Ciqin , Mu Rong , Wang Li , Cao Xiao-Ai , Yan Yan , Yu Ke 
Published: July 2024

AbstractAlkaline lakes are extreme environments inhabited by diverse microbial extremophiles. However, large-scale distribution patterns, environmental adaptations, community assembly, and evolutionary dynamics of microbial communities remain largely underexplored. This study investigated the characteristics of microbial communities on rare and abundant taxa in alkaline lake sediments in west and northwest China. We observed that abundant taxa varied significantly with geographical distance, while rare taxa remained unaffected by regional differences. The assembly process of abundant taxa was influenced by dispersal limitation, whilst rare taxa were predominantly driven by heterogeneous selection. Network analysis indicated that rare taxa as core species for community interactions and community stability. Rare taxa exhibited higher speciation and transition rate than abundant taxa, serving as a genetic reservoir and potential candidates to become abundance taxa, highlighting their crucial role in maintaining microbial diversity. These insights underscore the significant influence of rare taxa on ecosystem biodiversity and stability in alkaline lakes.
Novel nano composites from Citrus limon and Citrullus colocynthis agricultural wastes for biomedical applications
Authors: Kamel Nagwa A. , Wissa D. A. , Abd-El-Messieh Salwa L. 
Published: July 2024

AbstractIn recent years, academic and industrial research has focused on using agro-waste for energy and new material production to promote sustainable development and lessen environmental issues. In this study, new nanocomposites based on polyvinyl alcohol (PVA)-Starch using two affordable agricultural wastes, Citrus limon peels (LP) and Citrullus colocynthis (Cc) shells and seeds powders with different concentrations (2, 5, 10, and 15 wt%) as bio-fillers were prepared. The nanocomposites were characterized by Dielectric Spectroscopy, Fourier-Transform Infrared (FTIR), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and water swelling ratio. The antimicrobial properties of the nanocomposites against Escherichia coli, Staphylococcus aureus, and Candida albicans were examined to investigate the possibility of using such composites in biomedical applications. Additionally, the biocompatibility of the composites on human normal fibroblast cell lines (HSF) was tested using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. The results demonstrate that the filler type and concentration strongly affect the film's properties. The permittivity ε′, dielectric loss ε″ and conductivity σdc increased by increasing filler content but still in the insulators range that recommend such composites to be used in the insulation purposes. Both bio fillers control the water uptake, and the samples filled with LP were more water resistant. The polyvinyl alcohol/starch incorporated with 5 wt% LP and Cc have antimicrobial effects against all the tested microorganisms. Increasing the filler content has a negative impact on cell viability.
Pathologic complete response to KEYNOTE522 and HER2-directed therapy for synchronous TNBC and HER2+ breast cancer
Authors: Mai Nicholas , Chen Jie-Fu , Rana Satshil , Robson Mark , Chandarlapaty Sarat , Rosen Ezra Y. 
Published: July 2024

AbstractSimultaneous presentation of two separate primary breast cancers of differing histology at initial diagnosis is an uncommon phenomenon; it is even rarer to find these pathologically distinct populations within the same biopsy. Here we report the case of a patient diagnosed with clearly demarcated, pathologically heterogenous triple negative breast cancer (TNBC) and HER2+ breast cancer that was treated with a hybrid chemoimmunotherapy regimen combining elements of Keynote-522 and a standard HER2-directed neoadjuvant regimen, yielding apathologic complete response by the time of surgery with no notable adverse events. Molecular analysis of the histologically distinct tumor populations confirmed molecular evidence of differential HER2 expression but also suggested clonal relatedness of the two tumor populations based upon mutational profile, with phenotypic divergence potentially resulting from copy number alterations in NF1. Overall, this case highlights a rare histologic phenomenon that was successfully treated by combining both TNBC and HER2 directed neoadjuvant therapies.