bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promotion Effect of Catalpol on Angiogenesis and Potential Mechanisms: A Research Based on Network Pharmacology
Authors: Ni Jin‐rong , Zhang Qun‐hu , Deng Jie‐lin , Wang Hai‐hu , Duan Yong‐chi , Zhang Cheng‐ji , Jiang Lue‐tao 
Published: August 2024

ABSTRACTCatalpol, a natural iridoid glycoside, has potential therapeutic benefits, including anti‐inflammatory and neuroprotective effects. Investigating catalpol's role in angiogenesis is critical for understanding its potential therapeutic applications, particularly in diseases where modulating angiogenesis is beneficial. This study investigates catalpol's influence on angiogenesis and its mechanisms, combining network pharmacology and in vitro experiments. The target genes corresponding to the catalpol were analyzed by SwissTargetPrediction. Then angiogenesis‐related targets were acquired from databases like GeneCards. Subsequently, the Database for Annotation, Visualization and Integrated Discovery was employed for Gene Ontology and pathway analysis, while Cytoscape visualized protein interactions. The effect of catalpol on viability and angiogenesis of HUVECs was further examined using Cell Counting Kit‐8 and angiogenesis assays. RT‐qPCR and western blot were applied to check the expression of angiogenesis‐related proteins. Totally, 312 target genes of catalpol and 823 angiogenesis‐related targets were obtained with 56 common targets leading to PPI network analysis, highlighting hub genes (AKT1, EGFR, STAT3, MAPK3, and CASP3). These hub genes were mainly enriched in lipid and atherosclerosis pathway and EGFR‐related pathway. The in vitro experimental results showed that catalpol achieved a concentration‐dependent increase in HUVECs viability. Catalpol also promoted the migration and angiogenesis of HUVECs and up‐regulated the expression of EGFR. EGFR knockdown inhibited the effect of catalpol on HUVECs. Catalpol promotes angiogenesis in HUVECs by upregulating EGFR and angiogenesis‐related proteins, indicating its potential therapeutic application in vascular‐related diseases.
Cost‐Effectiveness of Digital Health Interventions for Asthma or COPD: Systematic Review
Authors: Ferreira Marta Alexandra Martins , dos Santos Adalberto Fernandes , Sousa‐Pinto Bernardo , Taborda‐Barata Luís 
Published: August 2024

ABSTRACTObjectiveDigital interventions such as remote monitoring of symptoms and physiological measurements have the potential to reduce the economic burden of asthma and chronic obstructive pulmonary disease (COPD) but their cost‐effectiveness remains unclear. This systematic review of randomised controlled trials (RCT) aims to assess whether digital health interventions can be cost‐effective in these patients.DesignSystematic review of RCTs. Study quality was assessed using RoB2 tool.Data SourcesSystematic search in three databases: PubMed, Scopus and Web of Science.Eligibility CriteriaStudies were eligible if they were RCTs with health economic evaluations assessing participants with asthma and/or COPD and comparing a digital health intervention to standard of care.ResultsWe included 35 RCTs, of which 21 were related to COPD, 13 to asthma and one to both diseases. Overall, studies assessed four categories of digital health interventions: (i) Electronic patient diaries (n = 4), (ii) real‐time monitoring (n = 19), (iii) teleconsultations (n = 6) and (iv) others (n = 6). Eleven studies performed a full economic evaluation analysis, while 24 studies performed a partial economic analysis. Most studies involving real‐time monitoring or teleconsultations presented economic results in favour of digital health interventions (indicating them to be cost‐effective or less expensive than the standard of care). Mixed results were obtained for electronic patient diaries. In the studies that conducted a full economic analysis, the incremental cost‐effectiveness ratio (ICER) ranged from 3530,93€/QALY and 286,369,28€/QALY. In the studies that conducted a partial economic analysis, the cost differences between the intervention group and the control group ranged from 0,12€ and 85,217,86€. Half studies with low risk of bias concluded that the intervention was economically favourable.ConclusionAlthough costs varied based on intervention type, follow‐up period and country, most studies report digital health interventions to be affordable or associated with decreased costs.Trial RegistrationPROSPERO: CRD42023439195
Brain border‐derived CXCL2+ neutrophils drive NET formation and impair vascular reperfusion following ischemic stroke
Authors: Huang Tingting , Guo Yunlu , Xie Wanqing , Yin Jiemin , Zhang Yueman , Chen Weijie , Huang Dan , Li Peiying 
Published: August 2024

AbstractBackgroundThe brain border compartments harbor a diverse population of immune cells and serve as invasion sites for leukocyte influx into the brain following CNS injury. However, how brain‐border myeloid cells affect stroke pathology remains poorly characterized.Methods and ResultsHere, we showed that ischemic stroke‐induced expansion of CXCL2+ neutrophils, which exhibit highly proinflammatory features. We tracked CXCL2+ neutrophils in vivo by utilizing a photoconvertible Kik‐GR mouse (fluorescent proteins Kikume Green Red, Kik‐GR) and found that brain‐infiltrating CXCL2+ neutrophils following ischemic stroke were mainly derived from the brain border rather than the periphery. We demonstrated that CXCL2 neutralization inhibited the formation and releasing of neutrophil extracellular traps (NETs) from in vitro cultured primary neutrophils. Furthermore, CXCL2‐neutralizing antibody treatment reduced brain infarcts and improved vascular reperfusion at day 3 postischemic stroke.ConclusionsCollectively, brain border‐derived CXCL2+ neutrophil expansion may impair vascular reperfusion by releasing NETs following ischemic stroke.
The repeat FIT (RFIT) study: Does repeating faecal immunochemical tests provide reassurance and improve colorectal cancer detection?
Authors: Farkas Nicholas G. , Palyvos Lampros , O'Brien James W. , Yu Kai Shing , Pigott Carolyn , Whyte Martin , Jourdan Iain , Rockall Timothy , Fraser Callum G. , Benton Sally C. 
Published: August 2024

AbstractAimFaecal immunochemical tests (FIT) are highly sensitive for colorectal cancer (CRC) detection. Little evidence exists regarding repeat FIT. The repeat FIT (RFIT) study aimed to determine whether second and third FIT provide reassurance and improve CRC or significant bowel disease (SBD) identification.MethodsThis was a prospective observational study. Patients recruited from urgent referrals returned three FIT and underwent colonoscopy. Chi‐square tests compared categorical data. Diagnostic accuracy variables (sensitivity/specificity/positive predictive value [PPV]/negative predictive value [NPV]) were calculated for one, two and three FIT (95% CI). Three negative FIT (<10 μg Hb/g of faeces [μg/g]) groups (one, two, three) were compared with positive groups (one or more FIT ≥10 μg/g). CRC and SBD detection rates were compared by strategy.ResultsA total of 460 patients (mean age: 66.8 years, 233 males and 227 females, 23 CRC, 80 SBD) were included in the study. For one, two and three negative FIT, CRC sensitivity remained static (95.7%); specificity (44.6%, 40.7% and 38.4%) and NPV decreased (99.5%, 99.4% and 99.4%). For SBD, sensitivity increased (78.8%, 83.8% and 86.3%), specificity decreased (47.4%, 43.7% and 41.6%) and NPV increased (91.4%, 92.7% and 93.5%). In one, two and three positive FIT groups, CRC detection was 8.3%,16.1% and 20.9%. CRC mean FIT was 150 μg/g, <6 μg/g for benign pathology.ConclusionsOne or more negative FIT increases the sensitivity for CRC/SBD. Repeating FIT provides greater differentiation of patients with and without CRC/SBD compared to single FIT but is associated with decreased specificity and PPV. Multiple negative FIT may offer reassurance; however, application of repeating FIT may be restricted given the associated increase in investigations.
Mesothelin CAR‐engineered NK cells derived from human embryonic stem cells suppress the progression of human ovarian cancer in animals
Authors: Liu Yanhong , Zhang Min , Shen Xiaoyan , Xia Chengxiang , Hu Fangxiao , Huang Dehao , Weng Qitong , Zhang Qi , Liu Lijuan , Zhu Yanping , Wang Lei , Hao Jie , Zhang Mengyun , Wang Tongjie , Wang Jinyong 
Published: August 2024

AbstractCAR‐NK cell therapy does not require HLA matching and has minimal side effects. However, traditional methods of engineering CARs into human tissue‐derived NK cells exhibit heterogeneity, low transduction efficiency, and high manufacturing costs. Here, we provide a reliable approach for generating large‐scale and cryopreserved mesothelin (MSLN) CAR‐NK cells from human embryonic stem cells (hESCs) as an alternative cell source. We first constructed MSLN CAR‐expressing hESCs to reduce CAR engineering costs and subsequently differentiated these stem cells into MSLN CAR‐NK cells via an efficient organoid induction system. The MSLN CAR‐NK cells exhibit the typical expression patterns of activating receptors, inhibitory receptors, and effector molecules of NK cells. In the presence of tumour cells, the MSLN CAR‐NK cells show increased secretion of IFN‐γ and TNF‐α, as well as elevated CD107a expression level compared with induced NK cells. We cryopreserved the MSLN CAR‐NK cells in liquid nitrogen using a clinical‐grade freezing medium (CS10) for more than 6 months to mimic an off‐the‐shelf CAR‐NK cell product. The thawed MSLN CAR‐NK cells immediately recovered after 48–72‐h culture and effectively eliminated ovarian tumour cells, including human primary ovarian tumour cells from patients. The thawed MSLN CAR‐NK cells efficiently suppressed ovarian tumour development in vivo and prolonged the survival of tumour‐bearing mice. Our study provides insights into the clinical translation of hESC‐derived MSLN CAR‐NK cells as a promising off‐the‐shelf cell product.
Epstein–Barr Virus‐Associated Smooth Muscle Tumor After Kidney Transplantation: A French Multicenter Retrospective Study
Authors: Tardieu Laurène , Anglicheau Dany , Sberro‐Soussan Rebecca , Lemoine Mathilde , Golbin Léonard , Fourdinier Ophélie , Bruneau Julie , Charbit Marina , Meatchi Tchao , Serre Jean‐Emmanuel , Le Quintrec Moglie , Karras Alexandre , Thervet Eric , Lazareth Hélène 
Published: August 2024

ABSTRACTBackgroundEpstein–Barr virus (EBV) is a herpesvirus linked to nine different human tumors and lymphoproliferative disorders. Immunosuppression promotes EBV‐driven malignancies. The most frequent EBV‐induced malignancies are lymphomas and nasopharyngeal carcinoma. By promoting smooth muscle proliferation, EBV can induce EBV‐associated smooth muscle tumors (EBV‐SMT). EBV‐SMT is a rare oncological entity for which no current guideline for diagnosis or management exists. Data on posttransplant EBV‐SMT (PT‐SMT) are scarce in kidney transplant recipients.MethodsWe conducted a national multicentric retrospective study and collected cases among transplantation centers in France. Kidney transplant recipients experiencing histologically proven PT‐SMT were included. We collected data on demographic characteristics of patient, history of kidney transplantation, history of PT‐SMT, evolution of graft function, and patient survival.ResultsEight patients were included. The median age at PT‐SMT diagnosis was 31 years (range 6.5–40). PT‐SMT occurred after a median delay of 37.8 months after transplantation (range 6–175). PT‐SMT management consisted in immunosuppressive regimen minimization in all patients. Introduction of mTOR inhibitors was performed in two patients. Four patients (50%) needed chemotherapy. Surgical resection was performed in four patients. At last follow‐up after PT‐SMT diagnosis (median 33 months (range 17–132)), five patients were considered in complete remission, and two patients had died. Two patients experienced graft rejection; two resumed dialysis (25%). All patients with available data presented with impaired graft function at last follow‐up.ConclusionPT‐SMT is a subacute and progressive disease during kidney transplantation. Even if the risk of developing PT‐SMT is low in kidney transplant recipients (0.07% in our cohort), PT‐SMT is associated with significant graft loss, possibly due to reduced immunosuppression. Developing guidelines could help transplantation teams better manage these patients.
The Impact of Donor‐Recipient Human Leukocyte Antigen Matching on Bronchiolitis Obliterans‐Free Survival Among Lung Transplant Recipients With Connective Tissue Diseases
Authors: Courtwright Andrew M. , Diamond Joshua M. , Sandorfi Nora , Goldberg Hilary J. 
Published: August 2024

ABSTRACTBackgroundThe development of connective tissue disease‐associated lung diseases (CTD‐LD) occurs in association with specific human leukocyte antigens (HLA). For CTD‐LD patients who require lung transplant, it is unknown whether utilization of donor organs expressing these same HLA impacts posttransplant outcomes.MethodsUsing the Scientific Registry of Transplant Recipients, we assessed whether CTD‐LD lung transplant recipients in the United States have worse bronchiolitis obliterans (BOS)‐free survival based on the degree of donor HLA matching. This included overall degree of donor‐recipient HLA matching, donor‐recipient matching at DR loci, and recipient matching with specific donor HLA antigens associated with the development of pulmonary disease in their condition.ResultsAmong 1413 patients with CTD‐ILD, highly HLA‐matched donor‐recipients did not have worse adjusted survival (hazard ratio [HR] = 0.93, 95% confidence interval [CI] = 0.58–1.51, p = 0.77). Recipients who were fully matched at HLA DR did not have worse survival (HR = 0.82, 95% CI = 0.56–1.19, p = 0.29). Finally, among individual CTD‐LD, including rheumatoid arthritis, systemic sclerosis, the idiopathic inflammatory myopathies, and systemic lupus erythematous, transplant with a donor expressing HLA antigens associated with lung manifestations in these conditions was not associated with worse BOS‐free survival.ConclusionsAmong transplant recipients with CTD‐LD, HLA donor‐recipient matching, including at the DR loci, does not result in worse BOS‐free survival. Based on these findings, there is no reason to treat these as unacceptable antigens when considering donor offers for CTD‐LD candidates.
Soundscapes and airborne laser scanning identify vegetation density and its interaction with elevation as main driver of bird diversity and community composition
Authors: Seibold Sebastian , Richter Tobias , Geres Lisa , Seidl Rupert , Martin Ralph , Mitesser Oliver , Senf Cornelius , Griem Lukas , Müller Jörg 
Published: August 2024

AbstractAimMountain ecosystems are hotspots of biodiversity due to their high variation in climate and habitats. Yet, above average rates of climate change and enhanced forest disturbance regimes alter local climatic conditions and vegetation structure, which should impact biodiversity. We here investigated the impact of vegetation and elevation as well as their interactions on bird communities to improve our ability to predict climate change effects on bird communities.LocationEuropean Alps, Germany.MethodsWe studied patterns and drivers of bird communities at 213 plots along gradients in vegetation density and elevation using autonomous sound recorders. Bird species were identified from soundscapes by Convolutional Neural Networks (BirdNET) and taxonomists.ResultsBird diversity and community metrics were moderately to strongly correlated for data based on either identification by BirdNET or taxonomists (Pearson's r = .47–.94), and ecological findings were overall similar for both datasets. Vegetation density 1–2 m and >2 m above ground strongly affected bird diversity and community composition and mediated effects of elevation. Community composition changed with elevation more strongly in habitats with low than high vegetation density >2 m. Species numbers decreased with elevation in habitats with low vegetation density 1–2 m and >2 m above ground, but increased in habitats with high vegetation density. Overall, functional and phylogenetic diversity increased with elevation indicating lower habitat filtering, but patterns were also mediated by vegetation density.Main ConclusionsOur results indicate that bird communities in the German Alps are determined by strong interactive effects of elevation and vegetation, underlining the importance to consider variation in vegetation in studies of biodiversity patterns along elevational gradients and under climate change. Combining remote sensing data and biodiversity monitoring based on autonomous sampling and AI‐based species identification opens new avenues for bird monitoring and research in remote areas.
Seasonal patterns of juvenile fish assemblages in the surf zones of tropical sandy beaches along Gaolong Bay, Hainan Island, China
Authors: Xia Wentong , Miao Zhongbo , Chen Kai , Lu Ying , Wang Sai , Zhu Junying , Xie Songguang 
Published: August 2024

AbstractAimSurf zones are crucial nursery habitats for the early life stages of fish species associated with typical coastal ecosystems. However, little is known about the temporal patterns and drivers of fish assemblages in tropical surf zones. This study aimed to assess the (1) main changes in fish community structure throughout 1 year, (2) seasonal dynamic patterns in fish assemblages, and (3) key factors influencing fish assemblages in the tropical surf zones.LocationGaolong Bay, Wenchang City, Hainan Island, China.MethodsFish sampling was conducted monthly from June 2021 to May 2022 using a beach seine net. Fish species were identified using both morphological and molecular analyses. Kruskal–Wallis test, analysis of similarity, non‐metric multidimensional scaling analysis, and similarity percentage analysis were used to investigate the temporal fish assemblage patterns. Generalised additive models and canonical correspondence analysis were used to assess how environmental variables influence fish assemblages.ResultsWe identified 83 fish species, which were grouped into three ecotypes based on their primary habitat: coral reef‐seagrass‐associated species (CS) (35), mangrove‐estuarine‐associated species (ME) (30), and common coastal‐estuarine‐associated species (CE) (18). Most captured individuals were juveniles, and fish abundance and diversity were highest in May. Most CS species were abundant between March and May. ME and most CE species were dominant from June to August, and Mugilidae (CE) was abundant between October and February. Furthermore, surf fish assemblages were substantially influenced by tidal level, water temperature, conductivity, pH, turbidity, and dissolved oxygen.ConclusionsJuvenile fish were abundant in May and fish species with three ecotypes alternate in the surf zones throughout the year. Counter to much current thinking, March maybe the spawn peak of most fish species in the studied area, and we suggest that the fishing ban period could start from March instead of May in the inshore areas of Hainan Island.
Comparing clinical outcomes in patients with type 2 diabetes mellitus after ischaemic stroke: Sodium–glucose cotransporter 2 inhibitors users versus non‐users. A propensity score matching National Cohort Study
Authors: Chen Tzu‐Yang , Lee Hsin‐Fu , Chan Yi‐Hsin , Chuang Chi , Li Pei‐Ru , Yeh Yung‐Hsin , Su Hung‐Chi , See Lai‐Chu 
Published: August 2024

AbstractAimThis nationwide cohort study evaluated the impact of sodium–glucose co‐transporter‐2 inhibitors (SGLT2i) on patients with type 2 diabetes mellitus (T2DM) after ischaemic stroke (IS), aiming to compare clinical outcomes between SGLT2i‐treated patients and those not receiving SGLT2i.Materials and MethodsUtilizing Taiwan's National Health Insurance Research Database, we identified 707 patients with T2DM treated with SGLT2i and 27 514 patients not treated with SGLT2i after an IS, respectively, from 1 May 2016 to 31 December 2019. Propensity score matching was applied to balance baseline characteristics. The follow‐up period extended from the index date (3 months after the index acute IS) until the independent occurrence of the study outcomes, 6 months after discontinuation of the index drug, or the end of the study period (31 December 2020), whichever came first.ResultsAfter propensity score matching, compared with the non‐SGLT2i group (n = 2813), the SGLT2i group (n = 707) exhibited significantly lower recurrent IS rates (3.605% per year vs. 5.897% per year; hazard ratio: 0.55; 95% confidence interval: 0.34–0.88; p = 0.0131) and a significant reduction in all‐cause mortality (5.396% per year vs. 7.489% per year; hazard ratio: 0.58; 95% confidence interval: 0.39–0.85; p = 0.0058). No significant differences were observed in the rates of acute myocardial infarction, cardiovascular death, heart failure hospitalization, or lower limb amputation.ConclusionsOur findings indicate significantly lower risks of recurrent IS and all‐cause mortality among patients with T2DM receiving SGLT2i treatment. Further studies are required to validate these results and investigate the underlying mechanisms behind the observed effects.
Propagule pressure from historic U.S. plant sales explains establishment but not invasion
Authors: Fertakos Matthew E. , Bradley Bethany A. 
Published: August 2024

AbstractIntroduction history, including propagule pressure and residence time, has been proposed as a primary driver of biological invasions. However, it is unclear whether introduction history increases the likelihood that a species will be invasive or only the likelihood that it will be established. Using a dataset of non‐native species historically available as ornamental plants in the conterminous United States, we investigated how introduction history relates to these stages of invasion. Introduction history was highly significant and a strong predictor of establishment, but only marginally significant and a poor predictor of invasive success. Propagule pressure predicted establishment better than residence time, with species likely to be established if they were introduced to only eight locations. These findings suggest that ongoing plant introductions will lead to widespread establishment but may not directly increase invasive success. Instead, other characteristics, like plant traits and local scale processes, may better predict whether a species becomes invasive.
Foundational Competencies in Educational Measurement: A Rejoinder
Authors: Ho Andrew D. , Ackerman Terry A. , Bandalos Deborah L. , Briggs Derek C. , Everson Howard T. , Lottridge Susan M. , Madison Matthew J. , Sinharay Sandip , Rodriguez Michael C. , Russell Michael , von Davier Alina A. , Wind Stefanie A. 
Published: August 2024

AbstractWhat are foundational competencies in educational measurement? We published a framework for these foundational competencies in this journal (Ackerman et al. 2024) and were grateful to receive eight commentaries raising a number of important questions about the framework and its implications. We identified five cross‐cutting recommendations among the eight commentaries relating to (1) our process and purpose, (2) Artificial Intelligence, (3) ethical competencies, (4) qualitative, critical, and culturally responsive commentaries, and (5) intersecting professions in, for example, classroom assessment and content development. In this rejoinder, we respond to these five recommendations and to each of the eight commentaries in turn. We hope that discussion and consensus about foundational competencies in educational measurement continue to advance in our journals and our field.