bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical descriptive and long‐term outcome of melanocytic uveal lesions in young dogs: 40 cases (45 eyes) including 13 cases of sector iridectomy
Authors: Dufour Valerie Liliane , Cohen Julie A. , Assenmacher Charles‐Antoine , Holle Dolores M. , Durham Amy C. , Aguirre Gustavo D. , Miyadera Keiko 
Published: July 2024

AbstractPurposeTo describe the clinical features surgical technique, early and long‐term outcome with or without surgery, and histopathological findings of melanocytic anterior uveal lesions in young dogs.MethodsMedical records of dogs at a guide dog facility removed from training due to a pigmented iris lesion were reviewed from 2014 to 2021. Selected dogs had surgical iridectomies performed.ResultsIridal melanocytic lesions were characterized as well‐delineated, pigmented, and flat (nevus) or raised (mass) lesions of the iris. Forty dogs (18 Labrador retrievers, 18 German shepherd dogs, 1 Golden retriever, 3 Labrador/Golden mixes) ranging from 0.5 to 3.1 years of age were affected unilaterally (n = 35) or bilaterally (n = 5). Sector iridectomy was performed in 13 dogs with prominent and well‐isolated mass lesion and enucleation was carried out in 2 dogs with extensive lesions, while all other cases were monitored without surgical intervention. Postoperative complications included dyscoria (13/13), focal posterior synechia (9/13) and focal nonprogressive cataract (8/13). All eyes remained visual and comfortable up to 6.2 years post‐iridectomy with no clinically identifiable local recurrence. Histopathology was consistent with uveal melanocytoma in all samples obtained surgically. All cases that did not undergo surgery remained free of complications up to 4.5 year post diagnosis.ConclusionMelanocytic anterior uveal lesions may be overrepresented in certain lineages of breeds and be present at a young age. While none of the eyes developed complications when monitored without surgery, early surgical excision of the mass by sector iridectomy yields noteworthy functional outcome and retention of a comfortable globe.
Respiratory modulation of sympathetic transduction to blood pressure in health and type 2 diabetes
Authors: Plunkett Michael J. , Holwerda Seth , Young Benjamin E. , Fadel Paul J. , Fisher James P. 
Published: July 2024

AbstractType 2 diabetes (T2D) is often accompanied by hypertension, exaggerated blood pressure (BP) responses to sympatho‐excitatory stressors, and raised cardiovascular disease risk. Appropriate respiratory–sympathetic coupling and sympathetic transduction to BP are important for short‐ and longer‐term BP control. We tested the hypotheses that respiratory modulation of muscle sympathetic nerve activity (MSNA) and its transduction to BP would be impaired in T2D and associated with higher BP and respiratory‐coupled BP variability. Resting MSNA, respiration and beat‐to‐beat BP were recorded in 20 T2D (49.1 ± 7.4 years; mean ± SD) and 13 healthy control (46.3 ± 9.4 years) participants. MSNA and the transduction of sympathetic bursts (signal‐averaging) to mean arterial pressure (MAP) were compared at low and high lung volume phases. The peak MAP response following a sympathetic burst was lower during the high lung volume than low lung volume phase in controls (P = 0.005), whereas it was unchanged with phase in T2D participants (P = 0.522). Respiratory modulation of MSNA was impaired in T2D participants, who had an attenuated reduction in burst incidence from low to the high lung volume phase, versus controls (27.8 ± 38.4% vs. 49.4 ± 24.6%, respectively; P = 0.043). The T2D participants were grouped into unimpaired respiratory modulators (burst incidence modulation median or above) or impaired respiratory modulators (below median). Impaired modulators had higher systolic BP (133 ± 14 vs. 121 ± 11 mmHg, P = 0.046), greater Traube–Hering wave amplitudes (6.3 ± 2.4 vs. 4.6 ± 1.1 mmHg; P = 0.028) and higher BP variability (MAP average real variability, 2.0 ± 0.7 vs. 1.4 ± 0.3, P = 0.033). Respiratory modulation of MSNA and sympathetic transduction to BP are altered in T2D patients and may contribute to their increased hypertension and cardiovascular risk.
imageKey points
Respiratory–sympathetic coupling and sympathetic transduction to blood pressure (BP) contribute to short‐ and longer‐term BP control. Our understanding of these processes in health and type 2 diabetes (T2D), a condition with high prevalence of hypertension and cardiovascular risk, is incomplete.
We found that respiration and sympathetic transduction to BP are coupled in healthy individuals. The mean arterial pressure response to a sympathetic burst was reduced during the high lung volume compared to the low lung volume phase. This coupling was absent in T2D.
Respiratory modulation of muscle sympathetic nerve activity (MSNA) is impaired in T2D, with a blunted reduction of MSNA observed during the high lung volume phase.
T2D patients with impaired respiratory MSNA modulation had augmented systolic BP, respiratory‐related BP excursions (Traube–Hering waves) and BP variability.
Abnormal respiratory modulation of MSNA and sympathetic transduction to BP in T2D may contribute to altered blood pressure control and cardiovascular risk in this population.
The Effectiveness of Content Knowledge Instruction in Tennis and Badminton in Physical Education Teacher Education
Authors: Chey Won Seok , Ward Phillip , Dillon Leslie , Watanabe Rio , Meyerhoff Sam , Arroyo-Rojas Fabián 
Published: July 2024

Purpose: Our research examined the effects of a content course in a physical education teacher education program. Methods: Ten participants were recruited from a physical education teacher education content course to examine participants’ common content knowledge (CCK) and specialized content knowledge (SCK) in tennis and badminton. Descriptive and inferential statistics were used to investigate (a) participants’ pre–post gains in CCK and SCK, (b) the relationship between participants’ playing and teaching experience with CCK and SCK, and (c) the relationship among the dependent variables in both pretest and posttest. Results: Participants’ content knowledge variables increased significantly. Furthermore, the study revealed inconsistent relationships between participants’ self-reported playing, teaching, and coaching experiences with their CCK and SCK scores at the pretest. We found inconsistent relationships between CCK and SCK among pre- and posttests. Discussion/Conclusion: The primary finding is that preservice teachers can improve substantively their content knowledge through well-designed content courses, but in some domains, there is more work to do.
How a Collaborative Parent–Adapted Physical Education Teacher Relationship Developed: A Retrospective Case Study
Authors: Forbes Adam S. , Block Martin E. 
Published: July 2024

Purpose: To explore the relationship between a mother and adapted physical education (APE) teacher. Method: A retrospective, qualitative case study approach was utilized. Semistructured interviews with the mother, child with Down syndrome, and APE teacher were conducted. Results: Four themes were extracted from the data: (a) “[APE] is perfectly normal”—value of APE, (b) “Education is power and key”—the importance of understanding, (c) “Whatever it takes to be a part of Sam’s day”—involvement in APE and PA, and (d) “Know your tribe and love them hard”—building strong relationships. Discussion: Findings suggest that parents and APE teachers have influence and contribute to the development of a collaborative relationship. From parents, parental value and importance toward APE were seen as facilitators. And from APE teachers, their communication by sharing visualizations of what occurs at school and their predispositions toward parents, such as competence and respect, were regarded as facilitators.
Quorum sensing positively regulates CPS-dependent
Autographiviridae
phage infection in
Vibrio alginolyticus
Authors: Li Xixi , Zhang Chen , Li Shenao , Liang Sixuan , Xu Xuefei , Zhao Zhe 
Published: July 2024

ABSTRACT

Quorum sensing (QS) orchestrates many bacterial behaviors, including virulence and biofilm formation, across bacterial populations. Nevertheless, the underlying mechanism by which QS regulates capsular polysaccharide (CPS)-dependent phage-bacterium interactions remains unclear. In this study, we report that QS upregulates the expression of CPS-dependent phage receptors, thus increasing phage adsorption and infection rates in
Vibrio alginolyticus
. We found that QS upregulated the expression of the
ugd
gene, leading to increased synthesis of
Autographiviridae
phage receptor CPS synthesis in
V. alginolyticus
. The signal molecule autoinducer-2 released by
Vibrio
from different sources can potentially enhance CPS-dependent phage infections. Therefore, our data suggest that inhibiting QS may reduce, rather than improve, the therapeutic efficacy of CPS-specific phages.

IMPORTANCE

Phage resistance is a direct threat to phage therapy, and understanding phage-host interactions, especially how bacteria block phage infection, is essential for developing successful phage therapy. In the present study, we demonstrate for the first time that
Vibrio alginolyticus
uses quorum sensing (QS) to promote capsular polysaccharide (CPS)-specific phage infection by upregulating
ugd
expression, which is necessary for the synthesis of
Autographiviridae
phage receptor CPS. Although increased CPS-specific phage susceptibility is a novel trade-off mediated by QS, it results in the upregulation of virulence factors, promoting biofilm development and enhanced capsular polysaccharide production in
V. alginolyticus
. This suggests that inhibiting QS may improve the effectiveness of antibiotic treatment, but it may also reduce the efficacy of phage therapy.
Gut microbiota influences onset of foraging-related behavior but not physiological hallmarks of division of labor in honeybees
Authors: Liberti Joanito , Frank Erik T. , Kay Tomas , Kesner Lucie , Monié--Ibanes Maverick , Quinn Andrew , Schmitt Thomas , Keller Laurent , Engel Philipp 
Published: July 2024

ABSTRACT

Gut microbes can impact cognition and behavior, but whether they regulate the division of labor in animal societies is unknown. We addressed this question using honeybees since they exhibit division of labor between nurses and foragers and because their gut microbiota can be manipulated. Using automated behavioral tracking and controlling for co-housing effects, we show that gut microbes influence the age at which bees start expressing foraging-like behaviors in the laboratory but have no effects on the time spent in a foraging arena and number of foraging trips. Moreover, the gut microbiota did not influence hallmarks of behavioral maturation such as body weight, cuticular hydrocarbon profile, hypopharyngeal gland size, gene expression, and the proportion of bees maturing into foragers. Overall, this study shows that the honeybee gut microbiota plays a role in controlling the onset of foraging-related behavior without permanent consequences on colony-level division of labor and several physiological hallmarks of behavioral maturation.

IMPORTANCE
The honeybee is emerging as a model system for studying gut microbiota-host interactions. Previous studies reported gut microbiota effects on multiple worker bee phenotypes, all of which change during behavioral maturation—the transition from nursing to foraging. We tested whether the documented effects may stem from an effect of the microbiota on behavioral maturation. The gut microbiota only subtly affected maturation: it accelerated the onset of foraging without affecting the overall proportion of foragers or their average output. We also found no effect of the microbiota on host weight, cuticular hydrocarbon (CHC) profile, hypopharyngeal gland size, and the expression of behavioral maturation-related genes. These results are inconsistent with previous studies reporting effects of the gut microbiota on bee weight and CHC profile. Our experiments revealed that co-housed bees tend to converge in behavior and physiology, suggesting that spurious associations may emerge when rearing environments are not replicated sufficiently or accounted for analytically.
Painfully ignorant? Impact of gender and aim of training on injuries in climbing
Authors: Grønhaug Gudmund , Saeterbakken Atle , Casucci Tallie 
Published: July 2024

IntroductionClimbing has evolved from an obscure outdoor sport to a predominantly indoor sport with the rise of mainstream climbing on artificial walls. Reported climbing-related injuries were predominantly chronic and may be avoided with proper planning of training. All climbers, regardless of age and gender, are training on the same routes and perform similar movements; however, few studies have investigated gender-specific injuries in climbing.ObjectivesAssess the distribution of chronic climbing injuries in an international population with gender-specific analyses and assess the impact of the person’s training focus or aim of training on those injuries.MethodsA cross-sectional survey using a web-based item-driven questionnaire was created and promoted using social media and several climbing media stakeholders. All climbers engaged in either sport climbing, bouldering or traditional climbing were included.ResultsThe survey received 1513 responses (877 men, 427 women and 9 not reporting gender), of which 50.3% (n=665; 51.4% men and 48.0% women) had experienced an injury in the past 12 months. There were significant differences in injuries in feet/ankle (p=0.014), neck (p=0.03), head (p=0.0001), shoulder (p=0.001), elbow (p=0.021) and fingers (p=0.003).ConclusionOver 50% of the climbers experienced an injury in the past 12 months. The most common injuries were to the shoulders (women) and fingers (men). There were significant differences between the genders regarding injury site and prevalence. The gender differences may be affected by the aim for training and the style of climbing.
Self-reported reproductive health of retired elite women’s footballers: a cross-sectional study
Authors: Carmody Sean , den Hollander Steve , Elliott-Sale Kirsty , Mountjoy Margo Lynn , Thornton Jane S , Massey Andrew , Kerkhoffs Gino , Gouttebarge Vincent 
Published: July 2024

ObjectivesThe primary objective of this study was to describe the self-reported reproductive health of retired elite women’s footballers with specific reference to menstrual function, pregnancy and motherhood, contraceptive use and pelvic floor function.MethodsAn electronic survey was disseminated to women’s footballers (18 years or older) who had retired from elite football within 10 years of completing the survey.Results69 respondents completed the survey (mean age 35.8 years, mean age at retirement 30.3 years). One-third of participants self-reported experiencing at least one episode of amenorrhoea (>3 months without menstruation) for reasons other than hormonal contraceptive use or pregnancy. Three participants (mean age of 41 years, range 30–54) reported having reached menopause at the time of the study. 54 (78.3%) participants were not using any form of contraception. 17 (24.6%) of the participants are mothers (range 1–3 children). 51 of the participants (73.9%) had never been pregnant, and the majority of those who had been pregnant (86.7%) became pregnant in less than 2 years following the onset of desire for pregnancy. Four of the retired players gave birth during their playing career, and the mean time to return to competitive matches following delivery was 22 weeks. The mean Pelvic Floor Distress Inventory-20 score for participants was 72.ConclusionThese insights can be used to inform future efforts to promote positive reproductive health outcomes for current, former and future women’s footballers. Research efforts should focus on improving the understanding of how to effectively support women’s footballers in the perinatal period. Best practice guidelines on the use of menstrual cycle monitoring and pelvic health support would improve standards of care for women’s footballers. Stakeholders should consider gender-specific postretirement care for women’s footballers.
Exploring markers of immunoresponsiveness in papillary thyroid carcinoma and future treatment strategies
Authors: Mohanty Atish , Afkhami Michelle , Reyes Amanda , Pharaon Rebecca , Yin Holly , Li Haiqing , Do Dana , Bell Diana , Nam Arin , Chang Sue , Gernon Thomas , Kang Robert , Amini Arya , Sampath Sagus , Kulkarni Prakash , Pillai Raju , Villaflor Vicky , Salgia Ravi , Maghami Ellie , Massarelli Erminia 
Published: July 2024

BackgroundThe study summarizes the potential use of immunotherapy forBRAF-mutated papillary thyroid cancer (PTC) by analyzing the immune profile of City of Hope PTC patient samples and comparing them to the thyroid dataset available in the TCGA database.Materials and methodsPTC cases with available formalin-fixed paraffin-embedded archived tumor tissue were identified. RNA was extracted from the tumor tissue and analyzed by NanoString to evaluate their immune gene expression profile. Immunohistochemistry was used to determine the expression of immune suppressive genes and lymphocytic infiltration into the tumor tissue. Thyroid cancer cell lines (MDA-T32, MDA-T68, MDA-T85, and MDA-T120) were used to determine the correlation between the BRAF inhibition and CD274 expression.ResultsThe study found that PTC cases withBRAFmutations had higher expression of immune checkpoint markers CD274 and CTLA4, as well as higher tumor-infiltrating lymphocytes, particularly CD4+T cells. Additionally, the study identified immunosuppressive markers expressed by tumor cells like CD73, CD276, and CD200 that could be targeted for immunotherapy. Further experiments using PTC cell lines lead to the conclusion that CD274 expression correlates withBRAFactivity and that inhibitors ofBRAFcould potentially be used in combination with immunotherapy to treat PTC.ConclusionsThese findings suggest that PTC cases withBRAFmutations or high expression may be correlated with an immune hot signature and could benefit from immunotherapeutic strategies.
In many voices: exploring end-of-life care through patient, caregiver and physician narratives
Authors: Moniz Tracy , Melro Carolyn , Watling Chris 
Published: July 2024

As the focus on end-of-life care intensifies, so too does the need to better understand the experiences of patients, caregivers and physicians. Delivering empathetic care requires a shared understanding of illness and its meaning and impact. The narratives of patients, caregivers and physicians each offer a distinct perspective on clinical experiences, yet comparative research is uncommon. This study compares written narratives about end-of-life illness and care by these groups.We created an archive of 332 first-person written narratives about end of life (patient=65, caregiver=156, physician=111) published between 1 January 2010 and 31 December 2019 through searching public domains (eg, national newspapers), personal blogs, and academic and literary journals in Canada. A comparative narrative analysis was conducted for patterns of content (eg, theme) and strategy (eg, characterisation).All three groups wrote about feeling gratitude. Patients also emphasised coping with change and carrying on. Caregivers further focused on grieving loss, and physicians most often wrote about valuing humanism. Physicians were most likely to ascribe agency to someone (ie, patients) or something (ie, death) other than themselves and to decentre themselves in the story. Patients and physicians most often made the patient the main character of their stories, while caregivers were as likely to centre the story on themselves as on the patient. Physicians were most likely to describe death as a source of tension, while patients and caregivers described the illness experience, often comparing it to battle. Physicians and caregivers tended to write testimonies, while patients wrote quests.Narrative research can illuminate unique aspects of end-of-life care. While death is a shared experience, each group approaches it differently. The disconnects have potential consequences for how end of life is experienced—whether patients’ values are honoured, whether caregivers receive support, and whether physicians experience burn-out. We need to foster learning experiences that integrate these unique perspectives into medical education and practice, including leveraging the affordances of studying written narratives towards this end.
Structural and Magnetic Properties of Y0.85La0.15Fe1−(4/3)xTixO3 (where (x = 0, 0.025, 0.05 and 0.075) Nanomaterials Derived from Sol-Gel Route
Authors: Kalakonda Sajini , Dachuru Rama Sekhara Reddy , Gudela Krishnaveni 
Published: July 2024

Y0.85La0.15Fe1−(4/3)xTixO3 (where (x = 0, 0.025, 0.05 and 0.075) nanomaterials were prepared using tartare acid assisted sol-gel method. Various analytical characterization techniques such as X-ray powder diffraction (XRD), scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV–vis absorption, Vibrating-sample magnetometer (VSM) and dielectric spectroscopy were employed to characterize the synthesized nanomaterials. Downward shifted of (121) and (002) planes were observed with an increasing of Ti concentration at Fe site through XRD study. The ability of the prepared nano-powders to absorb visible light was demonstrated by the investigation of diffuse reflectance spectra, which showed a decline in bandgap from 2.13 eV to 2.06 eV. XPS studies revealed that Y and La atoms are existed in +3 oxidation state, Fe atom is existed in mixed (+2 and +3) oxidation state and Ti atom is existed in mixed (+3 and +4) oxidation state. The highest magnetization value was observed for x = 0.05 sample through VSM study. High dielectric constant, low dielectric loss and low conductivity values were also observed for Y0.85La0.15Fe1−(4/3)xTixO3 (where (x = 0.05)).
A peripherally acting μ-opioid receptor antagonist for treating opioid-associated tinnitus: A case report
Authors: Ogawa Satoru , Amaya Fumimasa 
Published: July 2024

Background: The use of opioids occasionally causes tinnitus. However, there is a paucity of data regarding the use of peripherally acting μ-opioid receptor antagonists for opioid-associated tinnitus in patients with cancer. Actual case: A 74-year-old male with pancreatic cancer complained of abdominal pain. Two days after initiating oxycodone therapy, the patient experienced tinnitus during body movements. Although peripheral tinnitus disappeared after discontinuing oxycodone, it reappeared with hydromorphone or tapentadol administration. Possible courses of action: Drug cessation is a preferred intervention for drug-induced tinnitus; however, the cessation of opioids may not be feasible in patients with cancer who are already taking opioids. Formulation of a plan: Based on the presumed mechanism of peripheral tinnitus, the use of peripherally acting μ-opioid receptor antagonists was planned, and 200 μg/day of naldemedine was prescribed for tinnitus relief. Outcome: Tinnitus disappeared immediately after initiating naldemedine, and the pain was well-controlled. The effect was preserved after increasing or switching opioids. Lessons: The use of peripherally acting μ-opioid receptor antagonists may be an option to treat opioid-associated tinnitus without compromising the analgesic effects. View: Further clinical data regarding the secondary effect of peripherally acting μ-opioid receptor antagonists on opioid-associated complications other than constipation are required.