bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinematic performance of fine motor control in attention‐deficit/hyperactivity disorder: The effects of comorbid developmental coordination disorder and core symptoms
Authors: Lee I‐Ching , Chen Yung‐Jung , Tsai Chin‐Liang 
Published: November 2012

AbstractBackgroundThe aims of this study were: (i) to determine whether differences exist in the fine motor fluency and flexibility of three groups (children with attention‐deficit/hyperactivity disorder [ADHD], children in whomADHDis comorbid with developmental coordination disorder [DCD] [denoted asADHD+DCD], and a typically developing control group); and (ii) to clarify whether the degree of severity of core symptoms affects performance.MethodsThePeabodyPictureVocabularyTest‐Revised, theBeery–BuktenicaDevelopmentTest ofVisual‐MotorIntegration and theMovementAssessmentBattery forChildren were used as prescreening tests. TheIntegratedVisual andAuditory+Plus test was utilized to assess subjects' attention. The redesigned fine motor tracking and pursuit tasks were administered to evaluate subjects' fine motor performance.ResultsNo significant difference was found when comparing the performance of theChildren withADHDand the typically developing group. Significant differences existed between children in whomADHDis comorbid withDCDand typically developing children.ConclusionsChildren withADHDdemonstrated proper fine motor fluency and flexibility, and deficient performance occurred whenADHDwas comorbid with developmental coordination disorder. Children withADHDhad more difficulty implementing closed‐loop movements that required higher levels of cognitive processing than those of their typically developing peers. Also, deficits in fine motor control were more pronounced whenADHDwas combined with movement coordination problems. The severity of core symptoms had a greater effect on children with ADHD's fine motor flexibility than did fluency performance. In children with pureADHD, unsmooth movement performance was highly related to the severity of core symptoms.
Complete Genome Sequence of Klebsiella pneumoniae Phage JD001
Authors: Cui Zelin , Shen Wenbin , Wang Zheng , Zhang Haotian , Me Rao , Wang Yanchun , Zeng Lingbin , Zhu Yongzhang , Qin Jinhong , He Ping , Guo Xiaokui 
Published: November 2012

ABSTRACT

Klebsiella pneumoniae
is a member of the family
Enterobacteriaceae
, opportunistic pathogens that are among the eight most prevalent infectious agents in hospitals. The emergence of multidrug-resistant strains of
K. pneumoniae
has became a public health problem globally. To develop an effective antimicrobial agent, we isolated a bacteriophage, named JD001, from seawater and sequenced its genome. Comparative genome analysis of phage JD001 with other
K. pneumoniae
bacteriophages revealed that phage JD001 has little similarity to previously published
K. pneumoniae
phages KP15, KP32, KP34, and phiKO2. Here we announce the complete genome sequence of JD001 and report major findings from the genomic analysis.
Complete Genome Sequencing of Four Geographically Diverse Strains of Batai Virus
Authors: Groseth Allison , Matsuno Keita , Dahlstrom Eric , Anzick Sarah L. , Porcella Stephen F. , Ebihara Hideki 
Published: November 2012

ABSTRACT

Batai virus (BATV) is a widely distributed but poorly studied member of the
Orthobunyavirus
genus in the family
Bunyaviridae
and is of particular interest as a known participant in natural reassortment events. Both research and surveillance efforts on this and other related viruses have been hampered by the lack of available full-length sequence data covering all three genomic segments. Here, we report the complete genome sequence of four BATV strains (MM2222, Chittoor/IG-20217, UgMP-6830, and MS50) isolated from various geographical locations. Based on these data, we have determined that strain MS50 is in fact unrelated to BATV and likely represents as a novel genotype in the genus
Orthobunyavirus
.
Complete Genome Sequencing of Mosquito and Human Isolates of Ngari Virus
Authors: Groseth Allison , Weisend Carla , Ebihara Hideki 
Published: November 2012

ABSTRACT
Ngari virus (NRIV) is a recently described, naturally occurring reassortant between two other orthobunyaviruses, Bunyamwera virus (BUNV) and Batai virus (BATV). Intriguingly, this reassortment was associated with the acquisition of heightened virulence, although the molecular basis for this is not understood. Here we report the first complete genome sequences of Ngari virus. We include five isolates from various geographical locations, as well as samples isolated from both mosquitos and human cases. Based on an analysis of these sequence data, NRIVs are clearly genetically distinct from all known BUNV and BATV strains but are very closely related to one another regardless of their source.
Complete Genome Sequence of Serratia plymuthica Bacteriophage ϕMAM1
Authors: Matilla Miguel A. , Salmond George P. C. 
Published: November 2012

ABSTRACT

A virulent bacteriophage (ϕMAM1) that infects
Serratia plymuthica
was isolated from the natural environment and characterized. Genomic sequence analysis revealed a circular double-stranded DNA sequence of 157,834 bp, encoding 198 proteins and 3 tRNAs. The ϕMAM1 genome shows high homology to previously reported ViI-like enterobacterial bacteriophage genomes.
Complete Genome Sequence of an Infectious Bronchitis Virus Chimera between Cocirculating Heterotypic Strains
Authors: He Kun , Li Meng , Wei Ping , Mo Mei-lan , Wei Tian-chao , Li Kang-ran 
Published: November 2012

ABSTRACT
To date, multiple serotypes and genotypes of infectious bronchitis virus (IBV) have been isolated and identified. In order to provide more information on the viral evolution of IBVs, a new virulent strain named GX-NN09032, isolated from Guangxi, China, in 2009, was sequenced, and phylogenetic and recombination analyses were conducted. Furthermore, potential recombination events associated with GX-NN09032 were found in four IBV strains, including GX-YL5, DY07, CK/CH/SD09/005, TC07-2. The present study suggested that GX-NN09032 might contribute to the emergence of modern IBV variants through recombination.
Complete Genome Sequence of Wide-Host-Range Staphylococcus aureus Phage JD007
Authors: Cui Zelin , Song Zhen , Wang Yanchun , Zeng Lingbin , Shen Wenbin , Wang Zheng , Li Qingtian , He Ping , Qin Jinhong , Guo Xiaokui 
Published: November 2012

ABSTRACT

Methicillin-resistant
Staphylococcus aureus
-related infections have become a serious problem worldwide. Bacteriophage therapy is an alternative approach against this threat.
S. aureus
phage JD007, which belongs to the
Myoviridae
family according to transmission electron microscopic imaging, could lyse nearly 30% of the
S. aureus
strains from Ruijin Hospital, Shanghai, China, and was isolated from chicken feces in Shanghai, China. The complete genome showed that JD007 is a linear, double-stranded DNA phage 141,836 bp in length with a GC content of 30.4% encoding 217 open reading frames. A BLAST search of the JD007 genome revealed that it was very similar to that of phage GH15.
Complete Genome Sequence of Porcine Reproductive and Respiratory Syndrome Virus Strain ZCYZ Isolated from Hybrid Wild Boars
Authors: Wu Jiaqiang , Liu Shaoning , Du Yijun , Gao Yingchun , Wang Guisheng , Wang Jinbao 
Published: November 2012

ABSTRACT
A serologic investigation of porcine reproductive and respiratory syndrome virus (PRRSV) in hybrid wild boar herds was conducted during 2008–2009. PRRSV isolates with novel genetic markers were recovered. Experimental infection of pigs indicated that hybrid wild boars are involved in the epidemiology of PRRSV.
Intonation Adapts to Lexical Tone: The Case of Kammu
Authors: Karlsson Anastasia , House David , Svantesson Jan-Olof 
Published: November 2012

AbstractIn this paper, we investigate how lexical tones interact with intonation, using data from the Austroasiatic language Kammu, one of few languages with two dialects whose only major phonological difference is the presence or absence of lexical tones. Northern (and Western) Kammu have developed tones in connection with the merger of voiceless and voiced initial consonants, while the non-tonal Eastern dialect kept the segmental opposition with no tones. We found the following prosodic hierarchy: (1) lexical tones, (2) phrase-final boundary tone, (3) focus marking. The results strongly suggest that the intonational systems of the two Kammu dialects are basically identical, and that the main differences between the dialects are adaptations of intonation patterns to the lexical tones when the identities of the tones are jeopardized.
Proteomic profiling of Rhizobium tropiciPRF 81: identification of conserved and specific responses to heat stress
Authors: Gomes Douglas Fabiano , Batista Jesiane Stefânia da Silva , Schiavon Aline Luiza , Andrade Diva Souza , Hungria Mariangela 
Published: November 2012

Abstract

Background

Rhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C.

Results
Two-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses.

Conclusions
Our data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species.
Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation
Authors: Dehelean Cristina A , Şoica Codruţa , Ledeţi Ionuţ , Aluaş Mihaela , Zupko Istvan , Gǎluşcan Atena , Cinta-Pinzaru Simona , Munteanu Melania 
Published: November 2012

Abstract

Background
Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.

Results
New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.

Conclusions
The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.
Sternoclavicular Joint Infection: Classification of Resection Defects and Reconstructive Algorithm
Authors: Joethy Janna , Lim Chong Hee , Koong Heng Nung , Tan Bien-Keem 
Published: November 2012

Background Aggressive treatment of sternoclavicular joint (SCJ) infection involves systemic antibiotics, surgical drainage and resection if indicated. The purpose of this paper is to describe a classification of post resectional SCJ defects and highlight our reconstructive algorithm. Defects were classified into A, where closure was possible often with the aid of topical negative pressure dressing; B, where parts of the manubrium, calvicular head, and first rib were excised; and C, where both clavicular, first ribs and most of the manubrium were resected.
Methods Twelve patients (age range, 42 to 72 years) over the last 8 years underwent reconstruction after SCJ infection. There was 1 case of a type A defect, 10 type B defects, and 1 type C defect. Reconstruction was performed using the pectoralis major flap in 6 cases (50%), the latissimus dorsi flap in 4 cases (33%), secondary closure in 1 case and; the latissimus and the rectus flap in 1 case.
Results All wounds healed uneventfully with no flap failure. Nine patients had good shoulder motion. Three patients with extensive clavicular resection had restricted shoulder abduction and were unable to abduct their arm past 90°. Internal and external rotation were not affected.
Conclusions We highlight our reconstructive algorithm which is summarised as follows: for an isolated type B SCJ defect we recommend the ipsilateral pectoralis major muscle for closure. For a type C bilateral defect, we suggest the latissimum dorsi flap. In cases of extensive infection where the thoracoacromial and internal mammary vessels are thrombosed, the pectoralis major and rectus abdominus cannot be used; and the latissimus dorsi flap is chosen.
Sample Preparation, Data Collection, and Preliminary Data Analysis in Biomolecular Solution X‐Ray Scattering
Authors: Grishaev Alexander 
Published: November 2012

AbstractIn addition to the classic methods of structural biology—X‐ray crystallography and NMR—solution X‐ray scattering (SAXS) is playing an increasingly important role in structural investigation of biological macromolecules. However, the simultaneous ease of SAXS data collection and sophistication of its data analysis tools can present challenges to the investigator. Any sample, whether pure or contaminated, whether monodisperse or polydisperse, will yield scattering data, and it is up to the user to ensure the absence of artifacts and to choose a proper structural modeling strategy. This unit discusses experimental aspects of X‐ray solution scattering, including sample preparation and data collection, as well as the steps in data processing and preliminary analysis required to ensure the absence of artifacts. The goal is to summarize everything than can go wrong with SAXS data measurement so the user can have confidence in the data before undertaking structural modeling. Curr. Protoc. Protein Sci. 70:17.14.1‐17.14.18. © 2012 by John Wiley & Sons, Inc.
Scattering
Authors: Gabrys Barbara , Tomlins Paul E. 
Published: November 2012

AbstractScattering experiments on polymer systems can provide not only important structural information, but also their dynamics. In this article we overview the theoretical and practical aspects of scattering applied for elastic/inelastic and coherent/incoherent cases. Details about X‐ray, neutron and light scattering instrumentation, (such as diffractometers for elastic scattering and spectrometers for inelastic scattering), sample geometry, correlation functions, and data treatment are presented. We conclude with examples showing representative scattering techniques used to characterize polymers conformation, phase size, and chain motion.
Direct Synthesis of Periodic Mesoporous SilicoBoron CarboNitride Frameworks via the Nanocasting from Ordered Mesoporous Silica with Boron‐Modified Polycarbosilazane
Authors: Bernard Samuel , Majoulet Olivier , Sandra Fabien , Malchere Annie , Miele Philippe 
Published: November 2012

AbstractPeriodic mesoporous SilicoBoron CarboNitride (Si3.0B1.0C3.9N1.8) frameworks with p6mm hexagonal symmetry are prepared by a direct nanocasting process of ordered mesoporous silica. After a double infiltration/thermal cross‐linking cycle with a boron‐modified polycarbosilazane (BmPS) of the type [B(C2H4SiCH3NH)3]n (C2H4=CHCH3, CH2CH2), the derived BmPS confined in the porous structure of the periodic mesoporous silica template is subjected to a thermal process up to 1000 °C under N2 to be converted into the Si/B/C/N phase. A short chemical etching with dilute HF eliminates the silica template, which results in the formation of an open, continuous and highly ordered 2D hexagonal Si3.0B1.0C3.9N1.8 framework as demonstrated by EDX, LA‐XRD, and HRTEM analyses. The highly ordered mesoporous Si3.0B1.0C3.9N1.8 ceramic displays a specific surface area of 337 m2 g−1, a high pore volume (0.55 cm3 g−1) and a narrow pore‐size distribution centered on 4.6 nm by N2 sorption with an amorphous network remaining stable during continuous heat‐treatment to 1400 °C in a nitrogen atmosphere.
Measurement of an Iso‐Curie Temperature Line of a CoCrMo Solid Solution by Magnetic Force Microscopy Imaging on a Diffusion Multiple
Authors: Zhao Ji‐Cheng , Xu Yunhui , Hartmann Uwe 
Published: November 2012

AbstractThe 24 °C iso‐Curie temperature line of a CoCrMo fcc solid solution is obtained by performing magnetic force microscopy (MFM) imaging on solid solution compositions created in a diffusion multiple. The MFM imaging clearly reveals the boundary that separates the paramagnetic region without magnetic domains from the ferromagnetic region with domains. Compositional analysis along the boundary yields a constant Curie temperature (24 °C) composition line. Such a measurement is more efficient than one‐alloy‐at‐a‐time tests and can be used to screen new ferromagnetic materials.
Strong Spin‐Lattice Coupling Through Oxygen Octahedral Rotation in Divalent Europium Perovskites
Authors: Akamatsu Hirofumi , Kumagai Yu , Oba Fumiyasu , Fujita Koji , Tanaka Katsuhisa , Tanaka Isao 
Published: November 2012

AbstractFirst‐principles calculations reveal that in divalent europium perovskites EuMO3 (M = Ti, Zr, and Hf), antiferromagnetic superexchange interactions via nd states of the B‐site M cations (n = 3, 4, and 5, respectively) are enhanced by rotations of the MO6 octahedra. The octahedral rotations involved in a structural change from cubic $ Pm{\bar 3}m $ to orthorhombic Pbnm structures not only reduce energy gaps between the Eu 4f and M nd bands but also point the M nd orbitals at the Eu sites, leading to a significant overlap between the M nd and Eu 4f orbitals. These results reveal that the octahedral rotations are indispensable for antiferromagnetic ordering observed for EuZrO3 and EuHfO3, and put these perovskites into a class of materials exhibiting a novel type of strong coupling between their magnetism and octahedral rotations.
New Horizons for Diagnostics and Therapeutic Applications of Graphene and Graphene Oxide
Authors: Feng Lingyan , Wu Li , Qu Xiaogang 
Published: November 2012

AbstractGraphene, a one‐atom‐thick two‐dimensional (2D) layer of sp2‐bonded carbon, has received worldwide attention owing to its extraordinary physical and chemical properties. Recently, great efforts have been devoted to explore potential applications of graphene and its oxide in life science, especially in disease‐related diagnostics, near‐Infrared (NIR) phototherapy and imaging. Here we will introduce recent advances and new horizons in this area, and focus on the rising progress on NIR photothermal therapy for cancer and Alzheimer's disease (AD), human telomerase detection, stem cell proliferation and differentiation on graphene substrate, diagnosis of cancer cell and related biomarkers, drug/nucleotide/peptide delivery and cell imaging, which have not been comprehensively reviewed. We hope to provide an outlook to the applications of graphene and its oxide, especially on the new horizons in this field, and inspire broader interests across various disciplines.
Synthesis of poly(oligo(ethylene glycol)methacrylate)‐functionalized membranes for thermally controlled drug delivery
Authors: Teixeira Francisco , Popa Ana Maria , Guimond Sébastien , Hegemann Dirk , Rossi René M. 
Published: November 2012

AbstractIn this work a robust method for grafting thermoresponsive poly(oligo(ethylene glycol) methacrylates) (pOEGMA) macromolecules from polymeric porous membranes and silicon surfaces is presented. Hydrophilic track‐etched polyester (PETE) membranes with submicrometer pore sizes and silicon dies were submitted to a plasma treatment, which successfully allowed the introduction of anchoring groups and further grafting of the initiator on the surface. The surface‐initiated polymerization of OEGMA was carried out by atom transfer radical polymerization (ATRP), yielding dense polymer brushes. Moreover, the temperature‐controlled transport of caffeine through the functionalized membranes was demonstrated and the influence of the pore morphology and immobilized polymer layer thickness on the permeation profile was investigated. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Preparation of oligo‐N‐isopropylacrylamide brushes with OH and COOH end‐groups via surface‐initiated NMP
Authors: Cimen Dilek , Caykara Tuncer 
Published: November 2012

AbstractFunctional brushes have recently emerged as an extremely versatile way to modify surface properties in a robust and controlled way. In this study, well‐defined, high density oligo‐N‐isopropylacrylamide (oligoNIPAM) brushes with OH and COOH end‐groups were fabricated through a reliable strategy by the combination of the self‐assembly of bimolecular macroazoinitiator on silicon surface and surface‐initiated nitroxide mediated polymerization of N‐isopropylacrylamide in the presence of chain transfer agent (i.e., 2‐mercaptoethanol or 3‐mercaptopropionic acid). The living polymerization produced silicon substrate‐coated with functional oligoNIPAM with a target molecular weight and a grafting density as high as 8.14 chains nm−2. The functional oligoNIPAM brushes can be employed for the adsorption of biomacromolecules such as DNA and proteins. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Design of a super‐ductile polypropylene/polycarbonate blend with high heat resistance by using reactive plasticizer
Authors: Matsumoto Koji , Nagai Masahiro , Hamakawa Kohei , Nishitsuji Shotaro , Inoue Takashi 
Published: November 2012

AbstractHigh‐performance blend of polypropylene (PP) and polycarbonate (PC) has not been explored. The difficulty is caused by the big differences in melt viscosity (PP: low viscosity vs. PC: high viscosity) and polarity (PP: nonpolar vs. PC: polar). We put forth a new approach using a reactive plasticizer which is preferentially soluble with PC and polymerizable by organic peroxide. As the plasticizer, diallyl phthalate and triallyl cyanurate (TAC) were used. By reactive extrusion of PP/PC/plasticizer/dicumyl peroxide (e.g., 80/14/6/0.12 wt. ratio), reaction‐induced phase decomposition took place in the dispersed PC particles to develop a regularly phase‐separated nanostructure and the graft copolymer of PP and polymerized plasticizer was in situ generated at the interface. The extruded blend showed an excellent ductile behavior with about 500%‐elongation at break. TAC was very effective to elevate the heat resistance. Then, a super‐ductile PP/PC blend with high heat resistance was successfully developed. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013