bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA Profile of Tumorigenic Cells During Carcinogenesis of Lung Adenocarcinoma
Authors: Zhao Zhen‐guo , Jin Jun‐yu , Zhang An‐mei , Zhang Lu‐ping , Wang Xin‐xin , Sun Jian‐guo , Chen Zheng‐tang 
Published: October 2014

ABSTRACTTo obtain microRNA (miRNA) profile and clarify their biological function in tumorigenic Sca‐1+CD34+ cells during carcinogenesis of lung adenocarcinoma. After intranasal infection with recombinant Adeno‐Cre viruses (AdV‐Cre), lung adenocarcinoma was identified pathologically in Lox‐stop‐lox Kras (LSL‐Kras) G12D mice. Sca‐1+CD34+ cells were sorted by flow cytometry and tested for tumor‐initiating ability, self‐renewal and tumorigenicity. MiRNA profiles were obtained using microarray and further confirmed by real‐time RT‐PCR (qRT‐PCR). MiRNA functions were predicted bioinformatically, and miR‐294 function was verified to explore its role in tumor migration and invasion. Lung adenocarcinoma was induced in LSL‐Kras G12D mice within 30 days. In vivo, the tumorigenicity of Sca‐1+CD34+ cells was 25 times stronger than Sca‐1−CD34− cells. During tumorigenesis of lung adenocarcinoma, the expression of 145 miRNAs in Sca‐1+CD34+ cells increased and 72 miRNAs decreased (P < 0.01). Four successively up‐regulated miRNAs (miR‐15a*, miR‐203, miR‐294 and miR‐295*) and three successively down‐regulated ones (miR‐19b, miR‐483 and miR‐615–5p) were identified. Among them, miR‐294 could constitutively bind to 3'‐UTR of matrix metalloproteinase 3 (MMP3), and down‐regulate MMP3 protein expression. MiR‐294 also significantly inhibited migration and invasion of Lewis lung cancer cells. MiRNAs are characteristically expressed in tumor‐initiating Sca‐1+CD34+ cells of lung adenocarcinoma, and may play important roles during the carcinogenesis of lung adenocarcinoma. J. Cell. Biochem. 116: 458–466, 2015. © 2014 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc.
Spatio‐temporal variability of droughts in Bolivia: 1955–2012
Authors: Vicente‐Serrano S. M. , Chura O. , López‐Moreno J. I. , Azorin‐Molina C. , Sanchez‐Lorenzo A. , Aguilar E. , Moran‐Tejeda E. , Trujillo F. , Martínez R. , Nieto J. J. 
Published: October 2014

ABSTRACTIn this study, the spatio‐temporal variability and trends of droughts across Bolivia between 1955 and 2012 were investigated using two climate drought indices: the Standardized Precipitation Index (SPI), which is based on precipitation data, and the Standardized Precipitation Evapotranspiration Index (SPEI), which is based on the difference between the precipitation and the reference evapotranspiration (ETo). We found that the average drought conditions across the country showed a temporal behaviour mainly characterized by decadal variations. The spatial pattern of drought evolution showed marked differences between the Amazonian region and the Bolivian Altiplano. Both regions showed different drought periods, a lower frequency of drought variability in the Amazon region and trends towards drier conditions in the Altiplano, mainly due to a higher atmospheric water demand as a consequence of increased ETo. We also showed that inclusion of ETo, obtained from maximum and minimum temperature records, increased the spatial heterogeneity of the drought evolution in relation to the evolution observed when only precipitation droughts were considered. The SPEI, the calculation of which includes precipitation and ETo, indicated intensification in drought severity in the last years analysed relative to the pattern found when precipitation droughts alone were considered, and also indicated an increase in the magnitude and duration of drought events. The potential for increasing drought conditions under various climate change scenarios is discussed.
Anti‐inflammatory activity of mushroom‐derived hispidin through blocking of NF‐κB activation
Authors: Shao Hong Jun , Jeong Jin Boo , Kim Kui‐Jin , Lee Seong‐Ho 
Published: October 2014

AbstractBACKGROUNDHispidin, a polyphenol compound mainly derived from the valuable medicinal mushroom Phellinus species, has been found to possess distinct biological effects. However, the anti‐inflammatory potential of hispidin still remains uncharacterized.RESULTSIn this study, the effects of hispidin on activation of nuclear factor kappa B (NF‐κB) and the subsequent production of inducible nitric oxide synthase (iNOS) were determined in the lipopolysaccharide (LPS)‐induced macrophage RAW 264.7 cells. Our data indicated that hispidin inhibits transcriptional activity of NF‐κB in a dose‐dependent manner. Hispidin also attenuated LPS‐induced NF‐κB nuclear translocation and associated inhibitor of kappa B (IκB‐α) degradation. Furthermore, hispidin deceased iNOS protein expression and the generation of reactive oxygen species (ROS) in the LPS‐induced cells, but did not affect phosphorylation of mitogen‐activated protein kinases.CONCLUSIONThese findings suggest that hispidin exhibits anti‐inflammatory activity through suppressing ROS mediated NF‐κB pathway in mouse macrophage cells. © 2014 Society of Chemical Industry
Degradable Glycine‐Based Photo‐Polymerizable Polyphosphazenes for Use as Scaffolds for Tissue Regeneration
Authors: Rothemund Sandra , Aigner Tamara B. , Iturmendi Aitziber , Rigau Maria , Husár Branislav , Hildner Florian , Oberbauer Eleni , Prambauer Martina , Olawale Gbenga , Forstner Reinhard , Liska Robert , Schröder Klaus R. , Brüggemann Oliver , Teasdale Ian 
Published: October 2014

AbstractPhoto‐polymerizable scaffolds are designed and prepared via short chain poly(organo)phosphazene building blocks bearing glycine allylester moieties. The polyphosphazene was combined with a trifunctional thiol and divinylester in various ratios, followed by thiol‐ene photo‐polymerization to obtain porous matrices. Degradation studies under aqueous conditions showed increasing rates in correlation with the polyphosphazene content. Preliminary cell studies show the non‐cytotoxic nature of the polymers and their degradation products, as well as the cell adhesion and proliferation of adipose‐derived stem cells.
Doppler ultrasound compared with electrocardiogram and pulse oximetry cardiac triggering: A pilot study
Authors: Kording Fabian , Schoennagel Bjoern , Lund Gunnar , Ueberle Friedrich , Jung Caroline , Adam Gerhard , Yamamura Jin 
Published: October 2014

PurposeAccurate triggering of the cardiac cycle is mandatory for optimal image acquisition and thus diagnostic quality in cardiac magnetic resonance imaging. The purpose of this work was to evaluate Doppler ultrasound as an alternative trigger method in cardiac MRI.MethodsSteady‐state free precession (SSFP) 2D cine CMR was performed in 11 healthy subjects at 1.5T. Doppler ultrasound (DUS), electrocardiogram (ECG) and pulse oximetry (POX) were used for cardiac triggering. DUS peak detection was verified in comparison to ECG. Quantitative analysis of image quality of each gating method was determined by calculating endocardial border sharpness (EBS) and left ventricular (LV) function parameters and compared with ECG.ResultsMean difference between DUS and ECG in detected RR intervals was 0.04 ± 63 ms (r = 0.96). Trigger jitter was not different between ECG and DUS (P = 0.15) but significant different between ECG and POX (P = 0.01). EBS was similar between each method (3.1 ± 0.2 / 2.6 ± 0.2 / 2.9 ± 0.2 pixel). Mean differences in stroke volume were not significantly different with −1 ± 7 mL (ECG/DUS, P = 0.9) and 2 ± 10 mL (ECG/POX, P = 0.8).ConclusionCine cardiac MRI using DUS was successfully demonstrated. DUS triggering is an alternative method for cardiac MRI and may be applied in a clinical setting. Magn Reson Med 74:1257–1265, 2015. © 2014 Wiley Periodicals, Inc.
Computational modeling of membrane proteins
Authors: Koehler Leman Julia , Ulmschneider Martin B. , Gray Jeffrey J. 
Published: October 2014

ABSTRACTThe determination of membrane protein (MP) structures has always trailed that of soluble proteins due to difficulties in their overexpression, reconstitution into membrane mimetics, and subsequent structure determination. The percentage of MP structures in the protein databank (PDB) has been at a constant 1–2% for the last decade. In contrast, over half of all drugs target MPs, only highlighting how little we understand about drug‐specific effects in the human body. To reduce this gap, researchers have attempted to predict structural features of MPs even before the first structure was experimentally elucidated. In this review, we present current computational methods to predict MP structure, starting with secondary structure prediction, prediction of trans‐membrane spans, and topology. Even though these methods generate reliable predictions, challenges such as predicting kinks or precise beginnings and ends of secondary structure elements are still waiting to be addressed. We describe recent developments in the prediction of 3D structures of both α‐helical MPs as well as β‐barrels using comparative modeling techniques, de novo methods, and molecular dynamics (MD) simulations. The increase of MP structures has (1) facilitated comparative modeling due to availability of more and better templates, and (2) improved the statistics for knowledge‐based scoring functions. Moreover, de novo methods have benefited from the use of correlated mutations as restraints. Finally, we outline current advances that will likely shape the field in the forthcoming decade. Proteins 2015; 83:1–24. © 2014 Wiley Periodicals, Inc.
A survey of erasable itemset mining algorithms
Authors: Le Tuong , Vo Bay , Nguyen Giang 
Published: October 2014

Pattern mining, one of the most important problems in data mining, involves finding existing patterns in data. This article provides a survey of the available literature on a variant of pattern mining, namely erasable itemset (EI) mining. EI mining was first presented in 2009 and META is the first algorithm to solve this problem. Since then, a number of algorithms, such as VME, MERIT, and dMERIT+, have been proposed for mining EI. MEI, proposed in 2014, is currently the best algorithm for mining EIs. In this study, the META, VME, MERIT, dMERIT+, and MEI algorithms are described and compared in terms of mining time and memory usage. WIREs Data Mining Knowl Discov 2014, 4:356–379. doi: 10.1002/widm.1137This article is categorized under:

Algorithmic Development > Association Rules
Why Do Older People Change Their Ratings of Childhood Health?
Authors: Vuolo Mike , Ferraro Kenneth F. , Morton Patricia M. , Yang Ting-Ying 
Published: October 2014

Abstract
A growing number of studies in life course epidemiology and biodemography make use of a retrospective question tapping self-rated childhood health to assess overall physical health status. Analyzing repeated measures of self-rated childhood health from the Health and Retirement Study (HRS), this study examines several possible explanations for why respondents might change their ratings of childhood health. Results reveal that nearly one-half of the sample revised their rating of childhood health during the 10-year observation period. Whites and relatively advantaged older adults—those with more socioeconomic resources and better memory—were less likely to revise their rating of childhood health, while those who experienced multiple childhood health problems were more likely to revise their childhood health rating, either positively or negatively. Changes in current self-rated health and several incident physical health problems were also related to the revision of one’s rating of childhood health, while the development of psychological disorders was associated with more negative revised ratings. We then illustrate the impact that these changes may have on an adult outcomes: namely, depressive symptoms. Whereas adult ratings of childhood health are likely to change over time, we recommend their use only if adjusting for factors associated with these changes, such as memory, psychological disorder, adult self-rated health, and socioeconomic resources.
Locking Plate for AO Type C Intra-articular Distal Radius Fracture: Volar or Dorsal Approach?
Authors: Ka-Ho Tsang , Kai-Yiu Choi , Yat-Fai Chan , Shun-Chung Lau , Yuk-Yin Chow 
Published: October 2014

Purpose There is controversy over the outcomes and complications of volar and dorsal plating for the treatment of intra-articular fracture distal radius. Methods From 2008 to 2010, 81 patients with intra-articular fracture distal radius of AO type C1–C3 treated with distal radius locking plates via volar or dorsal approaches were reviewed in our institute. The clinical, radiological, and functional outcomes were evaluated at 6 months after operation. Results The volar approach group showed a significantly better flexion range, flexion-extension arc as well as Green and O'Brien functional score than dorsal approach group. Volar tilting of the distal radius was significantly better in the dorsal group, but that did not contribute to better palmar—flexion range or grip strength. Overall complication rate was similar in both groups. Conclusion The volar approach group demonstrated better range of motion and functional score. The complication rates were similar between the two groups. The volar surgical approach should be adopted in most operative cases of AO type C intra-articular fracture distal radius while the dorsal approach should be reserved for intra-articular fracture with dorsal comminuted fragments.
Flow past a transversely rotating sphere at Reynolds numbers above the laminar regime
Authors: Poon Eric K. W. , Ooi Andrew S. H. , Giacobello Matteo , Iaccarino Gianluca , Chung Daniel 
Published: October 2014

AbstractThe flow past a transversely rotating sphere at Reynolds numbers of $\mathit{Re}=500{-}1000$ is directly simulated using an unstructured finite volume collocated code. The effect of rotation rate on the flow is studied by increasing the dimensionless rotation rate, ${\it\Omega}^{\ast }$, from 0 to 1.20, where ${\it\Omega}^{\ast }$ is the maximum sphere surface velocity normalised by the free stream velocity. This study investigates the marked unsteadiness of the flow structures at $\mathit{Re}=500{-}1000$. Comparison with previous numerical data (Giacobello et al., J. Fluid Mech., vol. 621, 2009, pp. 103–130; Kim, J. Mech. Sci. Technol., vol. 23, 2009, pp. 578–589) reveals a new flow regime, namely a ‘shear layer–stable foci’ regime, besides the widely reported ‘vortex shedding’ and ‘shear layer instability’ regimes. The ‘shear layer–stable foci’ regime is observed at $\mathit{Re}=500$ and ${\it\Omega}^{\ast }=1.00$; $\mathit{Re}=640{-}1000$ and ${\it\Omega}^{\ast }\geqslant 0.80$. In this flow regime, the shear layer on the advancing side of the sphere (where the sphere surface velocity vector opposes the free stream velocity) shortens significantly while fluid from the retreating side (opposite to the advancing side) is drawn towards the mid-plane normal to the peripheral velocity. This results in the formation of a stable focus near the onset of the shear layer instability. This stable focus becomes more pronounced with increasing $\mathit{Re}$ and ${\it\Omega}^{\ast }$. It increases the oscillation magnitude and decreases the oscillation frequency of the hydrodynamic forces.
The Arabic original of (ps.) Māshā'allāh'sLiber de orbe: its date and authorship
Authors: MIMURA TARO 
Published: October 2014

AbstractLiber de orbe, attributed to Māshā'allāh (d.c.815), a court astrologer of the Abbasid dynasty, was one of the earliest Latin sources of Aristotelian physics. Until recently, its Arabic original could not be identified among Arabic works. Through extensive examination of Arabic manuscripts on exact sciences, I found two manuscripts containing the Arabic text of this Latin work, although neither of them is ascribed to Māshā'allāh: Berlin, Staatsbibliothek zu Berlin, Ms. or. oct. 273, and Philadelphia, Pennsylvania University Library, MS LJS 439. In this paper, I describe these two manuscripts in great detail, so that I confirm their originality of theLiber de orbe, and then by analysing the contents of the Arabic text, I deny the attribution to Māshā'allāh, and identify the title and author asBook on the Configuration of the Orbby Dūnash ibn Tamīm, a disciple of Isaac Israeli (c.855–c.955).
Assessing the Unterberger test: introduction of a novel smartphone application
Authors: Whittaker M , Mathew A , Kanani R , Kanegaonkar R G 
Published: October 2014

AbstractBackground:Peripheral vestibular function is commonly assessed using the Unterberger test. Patients are asked to march on the spot and their extent of rotation is recorded. The sensitivity of this test depends on an assessor accurately estimating the degree of rotation. This study therefore aimed to compare observer estimates with a smartphone application (DplusR Balance) that accurately records rotation.Method:Twenty-five participants were asked to estimate the degree of rotation in 10 successive Unterberger tests performed by a volunteer.Results:The average difference between estimated and application recorded extent of rotation was 30°.Conclusion:Assessors poorly estimate the degree of rotation in this clinical test, to an extent sufficient to affect clinical interpretation and diagnosis. We recommend the use of this application or alternative methods to record the degree of rotation in patients.
Efficacy of bilateral repetitive transcranial magnetic stimulation for negative symptoms of schizophrenia: results of a multicenter double-blind randomized controlled trial
Authors: Dlabac-de Lange J. J. , Bais L. , van Es F. D. , Visser B. G. J. , Reinink E. , Bakker B. , van den Heuvel E. R. , Aleman A. , Knegtering H. 
Published: October 2014

Background.Few studies have investigated the efficacy of repetitive transcranial magnetic stimulation (rTMS) treatment for negative symptoms of schizophrenia, reporting inconsistent results. We aimed to investigate whether 10 Hz stimulation of the bilateral dorsolateral prefrontal cortex during 3 weeks enhances treatment effects.Method.A multicenter double-blind randomized controlled trial was performed in 32 patients with schizophrenia or schizo-affective disorder, and moderate to severe negative symptoms [Positive and Negative Syndrome Scale (PANSS) negative subscale ⩾15]. Patients were randomized to a 3-week course of active or sham rTMS. Primary outcome was severity of negative symptoms as measured with the Scale for the Assessment of Negative Symptoms (SANS) and the PANSS negative symptom score. Secondary outcome measures included cognition, insight, quality of life and mood. Subjects were followed up at 4 weeks and at 3 months. For analysis of the data a mixed-effects linear model was used.Results.A significant improvement of the SANS in the active group compared with sham up to 3 months follow-up (p = 0.03) was found. The PANSS negative symptom scores did not show a significant change (p = 0.19). Of the cognitive tests, only one showed a significant improvement after rTMS as compared with sham. Finally, a significant change of insight was found with better scores in the treatment group.Conclusions.Bilateral 10 Hz prefrontal rTMS reduced negative symptoms, as measured with the SANS. More studies are needed to investigate optimal parameters for rTMS, the cognitive effects and the neural basis.
Cost-effectiveness of electroconvulsive therapy compared to repetitive transcranial magnetic stimulation for treatment-resistant severe depression: a decision model
Authors: Vallejo-Torres L. , Castilla I. , González N. , Hunter R. , Serrano-Pérez P. , Perestelo-Pérez L. 
Published: October 2014

BackgroundElectroconvulsive therapy (ECT) is widely applied to treat severe depression resistant to standard treatment. Results from previous studies comparing the cost-effectiveness of this technique with treatment alternatives such as repetitive transcranial magnetic stimulation (rTMS) are conflicting.MethodWe conducted a cost-effectiveness analysis comparing ECT alone, rTMS alone and rTMS followed by ECT when rTMS fails under the perspective of the Spanish National Health Service. The analysis is based on a Markov model which simulates the costs and health outcomes of individuals treated under these alternatives over a 12-month period. Data to populate this model were extracted and synthesized from a series of randomized controlled trials and other studies that have compared these techniques on the patient group of interest. We measure effectiveness using quality-adjusted life years (QALYs) and characterize the uncertainty using probabilistic sensitivity analyses.ResultsECT alone was found to be less costly and more effective than rTMS alone, while the strategy of providing rTMS followed by ECT when rTMS fails is the most expensive and effective option. The incremental cost per QALY gained of this latter strategy was found to be above the reference willingness-to-pay threshold used in these types of studies in Spain and other countries. The probability that ECT alone is the most cost-effective alternative was estimated to be around 70%.ConclusionsECT is likely to be the most cost-effective option in the treatment of resistant severe depression for a willingness to pay of €30 000 per QALY.
Rights to trade for species conservation: exploring the issue of the radiated tortoise in Madagascar
Authors: GANZHORN JÖRG U. , MANJOAZY THEODORE , PÄPLOW OLINGA , RANDRIANAVELONA ROMA , RAZAFIMANAHAKA JULIE H. , RONTO WILLIAM M. , VOGT ESTER , WÄTZOLD FRANK , WALKER RYAN C. J. 
Published: October 2014

SummaryIn many developing countries, people rely on natural resources for subsistence and cash income. The trade ban on species listed as critically endangered by the International Union for the Conservation of Nature (IUCN) Red List may be counter-productive, as increasing the rarity and thus price of these species acts as a stimulus to illegal markets rather than a deterrent. Since illegal markets cannot have legal property rights, there is no basis for any form of sustainable harvesting based on property rights. The Malagsy radiated tortoise (Astrochelys radiata) is an example of a species that is threatened by domestic and international trade; legalizing international trade could, under certain circumstances, provide financial incentives that might effectively reduce the domestic trade and subsistence harvest of this animal. This paper argues this critically-endangered species may be more effectively conserved by assigning trading rights to local communities, rather than demanding a trade ban that cannot be enforced successfully.
50 kGy of gamma irradiation does not affect the leachability of mineral soils and sediments
Authors: Gore Damian B. , Snape Ian 
Published: October 2014

Sterilization of soils and sediments can release them from quarantine restrictions. Gamma irradiation is effective at sterilization but can damage materials and in so doing affect their suitability for environmental research. Duplicate samples of a wide range of mineral soils and sediments were subject to an acetic acid extraction before and after 50 kGy gamma irradiation. This amount of gamma irradiation did not affect the leachability of a range of analytes from the soils and sediments.