bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteosarcoma Cells Enhance Angiogenesis Visualized by Color‐Coded Imaging in the In Vivo Gelfoam® Assay
Authors: Uehara Fuminari , Tome Yasunori , Miwa Shinji , Hiroshima Yukihiko , Yano Shuya , Yamamoto Mako , Mii Sumiyuki , Maehara Hiroki , Bouvet Michael , Kanaya Fuminori , Hoffman Robert M. 
Published: March 2014

ABSTRACTWe previously described a color‐coded imaging model that can quantify the length of nascent blood vessels using Gelfoam® implanted in nestin‐driven green fluorescent protein (ND‐GFP) nude mice. In ND‐GFP mice, nascent blood vessels are labeled with GFP. We report here that osteosarcoma cells promote angiogenesis in the Gelfoam® angiogenesis assay in ND‐GFP mice. Gelfoam® was initially transplanted subcutaneously in the flank of transgenic ND‐GFP nude mice. Seven days after transplantation of Gelfoam®, skin flaps were made and human 143B osteosarcoma cells expressing green fluorescent protein (GFP) in the nucleus and red fluorescent protein (RFP) in cytoplasm were injected into the transplanted Gelfoam®. The control‐group mice had only implanted Gelfoam®. Skin flaps were made at days 14, 21, and 28 after transplantation of the Gelfoam® to allow imaging of vascularization in the Gelfoam® using a variable‐magnification small animal imaging system and confocal fluorescence microscopy. ND‐GFP expressing nascent blood vessels penetrated and spread into the Gelfoam® in a time‐dependent manner in both control and osteosarcoma‐implanted mice. ND‐GFP expressing blood vessels in the Gelfoam® of the osteosarcoma‐implanted mice were associated with the cancer cells and larger and longer than in the Gelfoam®‐only implanted mice (P < 0.01). The results presented in this report demonstrate strong angiogenesis induction by osteosarcoma cells and suggest this process is a potential therapeutic target for this disease. J. Cell. Biochem. 115: 1490–1494, 2014. © 2014 Wiley Periodicals, Inc.
Differences in Nuclear DNA Organization Between Lymphocytes, Hodgkin and Reed–Sternberg Cells Revealed by Structured Illumination Microscopy
Authors: Righolt Christiaan H. , Guffei Amanda , Knecht Hans , Young Ian T. , Stallinga Sjoerd , van Vliet Lucas J. , Mai Sabine 
Published: March 2014

ABSTRACTAdvances in light microscopy have enabled the visualization of DNA in the interphase nucleus with more detail than is visible with conventional light microscopy. The nuclear architecture is assumed to be different in cancer cells compared to normal cells. In this paper we have studied, for the first time, the organization of nuclear DNA and that of DNA‐free space in control lymphocytes, Hodgkin cells and Reed–Sternberg cells using 3D structured illumination microscopy (SIM). We have observed detail in these SIM images that was not observed in conventional widefield images. We have measured the size distribution of the DNA structure using granulometry and noted a significant, progressive increase in the amount of sub‐micron structures from control lymphocytes to Hodgkin cells to Reed–Sternberg cells. The DNA‐free space changes as well; “holes” in the DNA distribution start to appear in the malignant cells. We have studied whether these “holes” are nucleoli by staining for upstream binding factor (UBF), a protein associated with the nucleolus. We have found that the relative UBF content progressively and significantly decreases—or is absent—in the DNA‐free space when measured as either the Pearson correlation coefficient with the DNA‐free space or as the number of “holes” that contain UBF. Similar differences exist within the population of Reed–Sternberg cells between binucleated and multinucleated cells with four or more subnuclei. To our knowledge, this is the first study that investigates the changes of the nuclear DNA structure in any disease with superresolution light microscopy. J. Cell. Biochem. 115: 1441–1448, 2014. © 2014 The Authors. Journal of Cellular Biochemistry published by Wiley Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
Glucosamine‐Induced Sp1 O‐GlcNAcylation Ameliorates Hypoxia‐Induced SGLT Dysfunction in Primary Cultured Renal Proximal Tubule Cells
Authors: Suh Han Na , Lee Yu Jin , Kim Mi Ok , Ryu Jung Min , Han Ho Jae 
Published: March 2014

AbstractThe aim of this study is to determine whether GlcN could recover the endoplasmic reticulum (ER) stress‐induced dysfunction of Na+/glucose cotransporter (SGLT) in renal proximal tubule cells (PTCs) under hypoxia. With the rabbit model, the renal ischemia induced tubulointerstitial abnormalities and decreased SGLTs expression in tubular brush‐border, which were recovered by GlcN. Thus, the protective mechanism of GlcN against renal ischemia was being examined by using PTCs. Hypoxia decreased the level of protein O‐GlcNAc and the expression of O‐GlcNAc transferase (OGT) while increased O‐GlcNAcase (OGA) and these were reversed by GlcN. Hypoxia also decreased the expression of SGLTs (SGLT1 and 2) and [14C]‐α‐methyl‐D‐glucopyranoside (α‐MG) uptake which were recovered by GlcN and PUGNAc (OGA inhibitor). Hypoxia enhanced reactive oxygen species (ROS) and then ER stress proteins, glucose‐regulated protein 78 (GRP78), and C/EBP‐homologous protein (CHOP). However, the expression of GRP78 increased till 6 h and then decreased whereas CHOP increased gradually. Moreover, decreased GRP78 and increased CHOP were reversed by NAC (antioxidant) and GlcN. GlcN ameliorated hypoxia‐induced decrease of O‐GlcNAc modification of Sp1 but OGT or Sp1 siRNAs blocked the recovery effect of GlcN on SGLT expression and α‐MG uptake. In addition, hypoxia‐decreased GRP78 and HIF‐1α expression was reversed by GlcN but OGT siRNA or Sp1 siRNA ameliorated the effect of GlcN. When PTCs were transfected with GRP78 siRNA or HIF‐1α siRNA, SGLT expression and α‐MG uptake was decreased. Taken together, these data suggest that GlcN‐induced O‐GlcNAc modified Sp1 with stimulating GRP78 and HIF‐1α activity ameliorate hypoxia‐induced SGLT dysfunction in renal PTCs. J. Cell. Physiol. 229: 1557–1568, 2014. © 2014 Wiley Periodicals, Inc.
Research and publication trends in hospital medicine
Authors: Do An N. Dang , Munchhof Amy M. , Terry Colin , Emmett Thomas , Kara Areeba 
Published: March 2014

BACKGROUNDResearch by hospitalists may aid the evolution of hospital medicine into an academic specialty.OBJECTIVETo describe the factors associated with research and publication activities among hospitalists and describe trends in hospitalist‐led publications.METHODSWe surveyed members of the Society of Hospital Medicine in June 2012 and conducted univariate analyses on their responses to determine predictors of successful authorship and to describe factors associated with research engagement. We searched PubMed from the database inception to October 2013 for publications with “hospitalist” or “hospital medicine” affiliated authors. Original research articles were reviewed for methodology and funding sources.RESULTSOf the 645 respondents (5.8% response rate), 277 (43%) had authored peer‐reviewed publications, 126 (19%) had access to mentorship, and 68 (11%) reported funding support. There were 213 (33%) who were engaged in research, with the majority conducting quality improvement (QI) research (n = 152, 24%). Completion of a fellowship, pediatrics training, the presence of a mentor, funding, and >25% protected time for research were each individually associated with an increased likelihood of authoring publications. Hospitalist‐led publications in PubMed have been increasing from 36 in 2006 to 179 in the first 10 months of 2013. Of the original research publications (n = 317), the majority were clinical (n = 129, 41%), and 58 (18%) were QI. Thirty‐nine (22%) authors reported funding support.CONCLUSIONSPeer‐reviewed publications by hospitalists are increasing, suggesting the academic maturation of hospital medicine. Provision of mentorship for hospitalists specifically in QI and guidance toward funding resources may assist in supporting this trend. Journal of Hospital Medicine 2014;9:148–154. © 2014 Society of Hospital Medicine
Automatic model‐based semantic registration of multimodal MRI knee data
Authors: Xue Ning , Doellinger Michael , Fripp Jurgen , Ho Charles P. , Surowiec Rachel K. , Schwarz Raphael 
Published: March 2014

PurposeTo propose a robust and automated model‐based semantic registration for the multimodal alignment of the knee bone and cartilage from three‐dimensional (3D) MR image data.Materials and MethodsThe movement of the knee joint can be semantically interpreted as a combination of movements of each bone. A semantic registration of the knee joint was implemented by separately reconstructing the rigid movements of the three bones. The proposed method was validated by registering 3D morphological MR datasets of 25 subjects into the corresponding T2 map datasets, and was compared with rigid and elastic methods using two criteria: the spatial overlap of the manually segmented cartilage and the distance between the same landmarks in the reference and target datasets.ResultsThe mean Dice Similarity Coefficient (DSC) of the overlapped cartilage segmentation was increased to 0.68 ± 0.1 (mean ± SD) and the landmark distance was reduced to 1.3 ± 0.3 mm after the proposed registration method. Both metrics were statistically superior to using rigid (DSC: 0.59 ± 0.12; landmark distance: 2.1 ± 0.4 mm) and elastic (DSC: 0.64 ± 0.11; landmark distance: 1.5 ± 0.5 mm) registrations.ConclusionThe proposed method is an efficient and robust approach for the automated registration between morphological knee datasets and T2 MRI relaxation maps. J. Magn. Reson. Imaging 2015;41:633–644. © 2014 Wiley Periodicals, Inc.
Apparent diffusion coefficient as a potential surrogate marker for Ki‐67 index in mucinous breast carcinoma
Authors: Onishi Natsuko , Kanao Shotaro , Kataoka Masako , Iima Mami , Sakaguchi Rena , Kawai Makiko , Kataoka Tatsuki R. , Mikami Yoshiki , Toi Masakazu , Togashi Kaori 
Published: March 2014

PurposeTo examine the association between apparent diffusion coefficient (ADC), cellularity, and Ki‐67 index in mucinous breast carcinoma (MBC) compared with invasive carcinoma of no special type (NST). ADC's ability to identify lesions with highly proliferating MBC was also examined.Materials and MethodsPathologically confirmed MBCs (mucinous group, n = 18) and NSTs (control group, n = 18) were retrospectively analyzed. ADC was calculated from signal intensity of diffusion‐weighted imaging at b values of 0 and 1000 sec/mm2. The Ki‐67 index and cellularity were histopathologically evaluated. The mucinous group was classified into high Ki‐67 mucinous group (Ki‐67 index ≥14%, highly proliferating) and low Ki‐67 mucinous group.ResultsIn the mucinous group, minimum ADC (ADCmin) showed an inverse correlation with cellularity (r = −0.802, P < 0.0001) and Ki‐67 index (r = −0.825, P < 0.0001). In the control group, ADCmin showed inverse correlation with cellularity (r = −0.537 P = 0.022), but no correlation with Ki‐67 index (r = 0.035, P = 0.892). ADCmin of high Ki‐67 mucinous group was significantly lower than that of low Ki‐67 mucinous group (P = 0.005).ConclusionThis study demonstrates an inverse correlation between ADC and Ki‐67 index in MBC and the ability of ADC to identify highly proliferating MBC. Considering that ADC can evaluate whole lesions noninvasively, ADC may be a promising noninvasive surrogate marker for Ki‐67 index in the risk stratification of MBC. J. Magn. Reson. Imaging 2015;41:610–615. © 2014 Wiley Periodicals, Inc.
A surface‐potential‐based model for silicon nanowire junctionless field‐effect transistors including interface traps
Authors: Deng Ye , Fan Xiao‐ya , Han Ru , Li Cong 
Published: March 2014

SUMMARYInterface trap is directly related to the electrical characteristics and reliability of the transistor, and it is an important factor affecting the performance of the device. This paper presents an analytic surface‐potential‐based model for cylindrical silicon nanowire junctionless field‐effect transistors (Si‐NW JLFET) including interface traps. In this paper, the proposed model is applicable to all Si‐NW JLFET working region including different interface trap charges with several nanowire radii and doping concentrations. Simulation results show that interface trap charges present two properties (positive or negative) depending on the different positions of the Fermi level in band gap, which have a significantly influence on the surface potential to cause junctionless transistor subthreshold degradation and flatband shifting. In addition, appropriate device parameters can effectively reduce the impact to the characteristic of the device made by the interface trap without degrading the performance of device. Copyright © 2014 John Wiley & Sons, Ltd.
Diurnal variation of precipitation in central Amazon Basin
Authors: Tanaka L. M. d. S. , Satyamurty P. , Machado L. A. T. 
Published: March 2014

ABSTRACTThe diurnal variation of precipitation at four stations in and around Manaus (03°S, 60°W) is studied using hourly observations for the period 2006–2011. The rainfall at midforest stations is 20% greater than that at the city stations, especially in the afternoon hours. The frequency of rain events varies substantially with the hour of the day, but the mean intensity of rain events does not. Daytime [06–18 local time (LT)] frequency is more than two times the nighttime (19–05 LT) frequency. The hours with highest frequency are 12–17 LT. The wet season November through May (NDJFMAM) frequency is approximately three times that of the dry season June through October (JJASO) frequency at all hours and at all stations. The diurnal frequency peak in the afternoon is more pronounced at the forest stations than at city stations. For smaller intensities of hourly rainfall the afternoon peaks are very pronounced while larger intensities of rainfall (15 mm h−1 and above) do not show preference for any hour of the day. Higher rainfall totals in the rainy season are due to more frequent rainfall rather than higher intensities of the rain events. Higher frequencies in the early morning hours close to the river are possibly due to the river breeze effect.
The transcription of nitrate transporters in upland rice varieties with contrasting nitrate‐uptake kinetics
Authors: Alberto Bucher Carlos , Azevedo Santos Leandro , de Matos Nogueira Eduardo , Passos Rangel Rafael , Regina de Souza Sonia , Silvestre Fernandes Manlio 
Published: March 2014

AbstractNitrogen (N) is one of the mineral elements required in the greatest quantity by plants and the one that most often limits productivity. In addition, the use of N fertilizers may be associated with environmental damage. The rational use of N fertilizers can be achieved by both better management techniques and the use of plant varieties that are more efficient in N use. Two rice (Oryza sativa L.) varieties (Piauí and IAC‐47) with contrasting nitrate‐uptake kinetics (Km and Vmax) were studied through the evaluation of the transcription of genes encoding for the high and low‐affinity nitrate transporters (NRT2 and NRT1, respectively) and NAR2 partner protein needed for NRT2 functional nitrate transporter, as well as the assessment of N soluble fractions (amino‐N, NO$ _3^- $‐N, NH$ _4^+ $‐N) and soluble sugars. The Piauí variety presented higher transcription of the OsNAR2.1, OsNRT2.1, OsNRT2.2 and OsNRT2.3a and accumulated more NO$ _3^- $ in the shoots, sheath and roots when cultivated under low supply of nitrate. OsNRT2.1, OsNRT2.2 and OsNAR2.1 transcription correlated with nitrate concentration in roots, while OsNRT2.1, OsNRT2.2 and OsNRT2.3a correlated with nitrate concentration in leaves. The results of this study indicate that the Piauí variety is adapted to conditions of low natural fertility and seasonal nitrate flux, characteristics of the humid tropics. There is evidence that a portion of this adaptation process maybe due to the differential expression of genes encoding for the high‐affinity nitrate transport proteins, synchronizing plant metabolism with nitrate availability in the soil.
Characterization of olive mill wastewater fractions treatment by integrated membrane process
Authors: Di Lecce Giuseppe , Cassano Alfredo , Bendini Alessandra , Conidi Carmela , Giorno Lidietta , Toschi Tullia Gallina 
Published: March 2014

AbstractBACKGROUNDUp to now, the management of olive mill wastewaters, a three‐phase mill by‐product, remains an unsolved problem, in particular for those regions where huge quantities of vegetable water are produced. Olive mill wastewaters were therefore treated to evaluate the characteristics of permeate and retentate fractions produced by an integrated membrane system working at two different volume concentration factors.RESULTSThe effect of two membrane‐based filtration steps (microfiltration and nanofiltration) on the content of chemical oxygen demand, dry matter, sensory quality, phenolic compounds and antioxidant activity of permeate and retentate samples was evaluated. Furthermore, the effect of two different volume concentration factors (VCF), in the nanofiltration step, were investigated. At high VCF values, the total phenolic content in the retentate fraction was found to be 3.7‐fold higher than the starting one, while the reduction of chemical oxygen demand in the permeate fraction was greater than 97% also at lower VCF values.CONCLUSIONEach filtration step has provided useful information concerning the utility and appropriateness of the processes chosen, suggesting a sustainable hypothesis of ‘normal industrial practice’ that can be included in current processes of oil extraction, in order to purify water and recover phenolic compounds with high added value. © 2014 Society of Chemical Industry
Surface‐Initiated RAFT Polymerization of Clay Nanoparticles with Polystyrene: New Insights Using MALDI‐TOF MS and 1H NMR
Authors: Chirowodza Helen , Hartmann Patrice C. , Pasch Harald 
Published: March 2014

Reversible addition‐fragmentation chain transfer (RAFT)‐mediated grafting of polystyrene from clay is investigated using advanced analytical methods to elucidate the polymerization mechanism occurring at the clay surface and in solution. The chain‐end structures of the grafted polymer grown from a surface‐attached cationic functionalized RAFT agent, and the free polymer chains generated from a non‐functionalized RAFT agent are investigated using size exclusion chromatography, matrix‐assisted laser desorption/ionization time‐of‐flight (MALDI‐TOF) mass spectrometry, MALDI‐TOF–collision‐induced dissociation mass spectrometry, and 1H NMR spectroscopy. The results obtained show that free and surface‐confined polymer chains undergo significant conventional chain‐transfer reactions in addition to reversible addition–fragmentation chain transfer. This information provides additional new insight into the polymerization mechanism.
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Electrochemical Post‐Functionalization of Conducting Polymers
Authors: Inagi Shinsuke , Fuchigami Toshio 
Published: March 2014

This article summarizes recent progress in the post‐functionalization of conjugated polymers by electrochemical methods. These electrochemical polymer reactions typically proceed via electrochemical doping of a conjugated polymer film, followed by chemical transformation. Examples include the quantitative oxidative fluorination of polyfluorenes and oxidative halogenation of polythiophenes, as well as the reductive hydrogenation of polyfluorenones. The degree of functionalization, otherwise known as the reaction ratio, can be controlled by varying the charge passed through the polymer, allowing the optoelectronic properties of the conjugated polymers to be tailored. Wireless bipolar electrodes with an in‐plane potential distribution are also useful with regard to the electrochemical doping and reaction of conjugated polymers and allow the synthesis of films exhibiting composition gradients. Such bipolar electrochemistry can induce multiple reaction sites during electrochemical polymer reactions.
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A Bi‐Objective Inventory Routing Problem for Sustainable Waste Management Under Uncertainty
Authors: Nolz Pamela C. , Absi Nabil , Feillet Dominique 
Published: March 2014

ABSTRACTThe fast increase in waste materials disposed of by home‐health and medical care services as well as by self‐attending patients leads to a major concern over the safe disposal of health care waste. In this paper, we consider the design of a collection system for infectious medical waste. These waste materials are produced by patients in self‐treatment, stored at pharmacies and picked up by local authorities for disposal.The stochastic waste collection problem is formulated considering two conflicting objective functions. Social objectives, specifically the satisfaction of pharmacists and local authorities involved in the collection process, as well as the minimization of public health risks need to be traded off against distribution costs comprising routing costs and fixed costs incurred each time a tour is planned. We propose three heuristic solution approaches to solve the real‐world motivated inventory routing problem. The suggested methods are tested on real‐world data from the region of Provence‐Alpes‐Côte d'Azur, in France, and their performance is evaluated with several quality indicators. Copyright © 2014 John Wiley & Sons, Ltd.
The impact of isolated single umbilical artery on labor and delivery outcome
Authors: Ashwal Eran , Melamed Nir , Hiersch Liran , Edel Sacha , Bardin Ron , Wiznitzer Arnon , Yogev Yariv 
Published: March 2014

ABSTRACTObjectiveData regarding the association between isolated single umbilical artery (SUA) and pregnancy outcome are inconsistent and mainly address the risk of pregnancy complications. Thus, we aimed to focus on the association between isolated SUA, and labor and delivery.MethodsWe conducted a case–control study of all singleton pregnancies diagnosed with isolated SUA (no known chromosomal/structural anomalies/fetal growth restriction) attempting vaginal delivery. Obstetric and neonatal outcome was compared with that of a control group of pregnancies with a three‐vessel cord in a 1:2 ratio matched by parity. Composite adverse outcome included the following: Cesarean section (CS) and/or operative delivery due to non‐reassuring fetal heart rate (NRFHR), prolonged neonatal admission, low Apgar score and umbilical artery pH < 7.2.ResultsOut of 34 196 deliveries, 162 (0.5%) were diagnosed with SUA, and 91 (0.27%) were diagnosed with isolated SUA. Isolated SUA was associated with a higher rate of CS due to NRFHR (5.5% vs 1%, p = 0.02), small for gestational age (SGA) (14.3% vs 4.9%, p = 0.009), lower birth weight, and a higher rate of composite adverse outcome (20.9% vs 8.8%, p = 0.005). On multivariable analysis, isolated SUA was independently associated with an increased risk for composite adverse outcome (OR 2.34, 95% CI 1.05–5.21).ConclusionIsolated SUA is associated with increased risk for CS due to NRFHR and increased rate of SGA. © 2014 John Wiley & Sons, Ltd.