bluuebunny/crossref_metadata_2025_split huggingface dataset:
The biological impact of concurrent exposure to metallic nanoparticles and a static magnetic field
Authors: Comfort Kristen K. , Maurer Elizabeth I. , Hussain Saber M. 
Published: May 2013

AbstractThe rapid advancement of technology has led to an exponential increase of both nanomaterial and magnetic field utilization in applications spanning a variety of sectors. While extensive work has focused on the impact of these two variables on biological systems independently, the existence of any synergistic effects following concurrent exposure has yet to be investigated. This study sought to ascertain the induced alterations to the stress and proliferation responses of the human adult low calcium, high temperature keratinocyte (HaCaT) cell line by the application of a static magnetic field (approximately 0.5 or 30 mT) in conjunction with either gold or iron oxide nanoparticles for a duration of 24 h. By evaluating targets at a cellular, protein, and genetic level a complete assessment of the HaCaT response was generated. A magnetic field‐dependent proliferative effect was found (∼15%), which correlated with a decrease in reactive oxygen species and a simultaneous increase in ki67 expression, all occurring independently of nanoparticle presence. Furthermore, the application of a static magnetic field was able to counteract the cellular stress response induced by nanoparticle exposure through a combination of decreased reactive oxygen species production and modification of gene regulation. Therefore, we conclude that while these variables each introduce the potential to uniquely influence physiological events, no negative synergistic reactions were identified. Bioelectromagnetics. 34:500–511. © 2013 Wiley Periodicals, Inc.
Reibungsfreie Gleitlagerung für vorgespannte Bodenplatten
Authors: Schweighofer Anton , Kollegger Johann 
Published: May 2013

AbstractAm Institut für Tragkonstruktionen – Betonbau der Technischen Universität Wien wurde eine neue Gleitlagerung entwickelt, die eine reibungslose Lagerung von vorgespannten Bodenplatten während des Herstellungsprozesses sicherstellt. Die Umsetzbarkeit dieser neuen Methode wurde in Großversuchen an 60 m langen, 1 m breiten und 30 cm dicken Betonplattenstreifen getestet. Die neue Gleitlagerung besteht aus zwei Lagen luftdichter Folien mit dazwischen liegendem Vlies. Die Folien werden an ihren Rändern durch Kleben oder Schweißen miteinander verbunden. Nach dem Herstellen der Bodenplatte wird im Zwischenraum der beiden Folien Überdruck erzeugt. Dieser Zwischenraum wird durch das eingelegte Vlies sichergestellt. Der erzeugte Überdruck, in der Höhe des Eigengewichts der Platte, ermöglicht eine reibungsfreie Verformung der Bodenplatte infolge abfließender Hydratationswärme, Schwinden und Vorspannung, ohne den Aufbau von Zwang oder Verluste der Vorspannkraft.Frictionless plain bearing for post‐tensioned slab on groundA new method for building large concrete slabs on ground on a frictionless support was developed at Vienna University of Technology. An Austrian patent for this method was granted and an international patent application has been filed. The feasibility of this new method has been demonstrated in large field tests on concrete slab strips with a length of 60 m, a width of 1.0 m and a thickness of 30 cm. The frictionless support is obtained by first placing an air‐tight membrane, then a geotextile and at last a second air‐tight membrane on the ground where the concrete slab is to be built. In the next step the membranes are sealed at the boundary. After casting the slab on top of the second membrane, low air pressure is applied in the clearance between the two membranes. This clearance is created by the geotextile. Shortening of the slab due to the loss of hydration heat, early shrinkage and post‐tensioning is possible without causing friction forces between slab and subsoil because the self‐weight of the slab is balanced by the applied air pressure.
Segmentbrücke aus textilbewehrtem Beton – Rottachsteg Kempten im Allgäu
Authors: Michler Harald 
Published: May 2013

AbstractAufgrund seiner sehr guten mechanischen Eigenschaften ist Textilbeton hervorragend geeignet, Leichtbau mit Beton zu betreiben. Damit ergibt sich die Möglichkeit, Betonbrücken von bisher nicht für möglich gehaltener Größe vorzufertigen und quasi fix und fertig an den Bestimmungsort zu transportieren. Damit ergeben sich kürzere Bauzeiten bzw. Verkehrssperrungen und auch wirtschaftliche und qualitative Vorteile lassen sich durch die Fertigung im Werk erzielen.Mit der in Oschatz/Sachsen hergestellten und im Herbst 2007 im Allgäu montierten Geh‐ und Radwegbrücke über die Rottach in Kempten im Allgäu wird dies in der Praxis bewiesen und gezeigt, dass sich Bauteile aus textilbewehrtem Beton auch wirtschaftlich am Markt durchsetzen können. Die Brücke wurde im Herbst 2007 in Betrieb genommen und hat sich nun schon im sechsten Winter bewährt. Dies wird zum Anlass genommen, um in diesem Beitrag über die Konzeption, die Berechnung und die Herstellung der Textilbetonbrücke Kempten zu berichten. Dieser Bericht basiert auf dem Aufsatz “Innovativ! Leicht! Formbar! Bewährt! – Textilbetonbrücke Rottachsteg Kempten im Allgäu”, der im Tagungsband zum 23. Dresdner Brückenbausymposium 2013 abgedruckt ist, und übernimmt Teile aus diesem.Segment bridge of textile reinforced concrete – Rottachsteg Kempten in the AllgäuOn account of its very good mechanical properties textile reinforced concrete (TRC) is a material to be best to do lightweight concrete constructions. With this material it is possible to realize prefabricated concrete bridges with a size that was not possible yet due to limits in transport. Shorter construction or traffic blockages times are also a positive effect and with this economic and qualitative advantages are achievable due to prefabrication.A praxis test was done with a bridge prefabricated in Oschatz/ Saxony and transported to Kempten/Allgäu for mounting. The Rottach‐Bridge does prove and demonstrate that textile reinforced concrete constructions can also assert themselves economically in the market. The bridge was put into operation in autumn 2007 and now has proved itself already in sixth winter. This is taken as an occasion to report in this paper on the design, calculation and preparation of textile concrete bridge Kempten. This report is based on and takes over parts from this.
Kinetic modeling of native Cassava starch thermo‐oxidative degradation using Weibull and Weibull‐derived models
Authors: Janković Bojan 
Published: May 2013

ABSTRACTA new approach in kinetic modeling of thermo‐oxidative degradation process of starch granules extracted from the Cassava roots was developed. Based on the thermoanalytical measurements, three reaction stages were detected. Using Weibull and Weibull‐derived (inverse) models, it was found that the first two reaction stages could be described with the change of apparent activation energy (Ea) on conversion fraction (α(T)) (using “Model‐free” analysis). It was found that first reaction stage, which involves dehydration and evaporation of lower molecular mass fractions, can be described with an inverse Weibull model. This model with its distribution of Ea values and derived distribution parameters includes the occurrence of three‐dimensional diffusion mechanism. The second reaction stage is very complex, and it was found to contain the system of simultaneous reactions (where depolymerization occurs), and can be described with standard Weibull model. Identified statistical model with its distribution of Ea values and derived distribution parameters includes the kinetic model that gives the variable reaction order values. Based on the established models, shelf‐life studies for first two stages were carried out. Shelf‐life testing has shown that optimal dehydration time is achieved by a programmed heating at medium heating rate, whereas optimal time of degradation is achieved at highest heating rate. © 2013 Wiley Periodicals, Inc. Biopolymers 101: 41–57, 2014.
Supercoiled pseudocircular domains in single‐twisted DNAs under tension: Elastic constants and unwinding dynamics in complexes with topo I
Authors: Schurr J. Michael , Fujimoto Bryant S. 
Published: May 2013

ABSTRACTExtension versus twist data of Koster et al. (Nature 2005, 434, 671–674) are analyzed to obtain C for the main‐chain segments and the twist energy parameter (ET) for the supercoiled pseudocircular (sp) domain(s) from which C is estimated via simulations. The torsional rigidity in the tension‐free sp domain(s) (C = 163 fJ fm) is typical of the unstrained DNA and is less than half the value in the main‐chain segments under tension (C = 350–410 fJ fm). Tension is suggested to induce a structural transition to a torsionally stiffer state. Data of Koster et al. for the rate of extension owing to unwinding of a covalent complex of DNA with human Topoisomerase Ib (H Topo I) are analyzed to determine the torque and rate of rotation from which an effective friction coefficient is obtained. A Langevin equation for the unwinding motion in a supercoiled DNA:H Topo I complex is solved to obtain the temporal trajectory of the average winding angle and the time‐dependent distribution of winding angles. The mean rate constant for the religation reaction is estimated from the measured probability of reaction per turn. We predict that unwinding proceeds rather far during a single‐cleavage and religation cycle, and is effectively completely equilibrated during the 3.2 cleavage and religation cycles that occur during each noncovalent binding and dissociation event. H Topo I is predicted to be completely processive as in accord with observations on calf‐thymus Topo I (Brewood et al., Biochemistry 2010, 49, 3367–3380). © 2013 Wiley Periodicals, Inc. Biopolymers 99: 1046–1069, 2013.
Story in a sample—the potential (and limitations) of cryo‐electron microscopy applied to molecular machines
Authors: Frank Joachim 
Published: May 2013

ABSTRACTThis article addresses recent developments in cryo‐electron microscopy and single‐particle reconstruction of macromolecules. With the advent of powerful classification techniques, it is now possible to extract and visualize multiple conformers contained within the same dataset. It is discussed how and to what extent this technique can be used in the study of the dynamics of molecular machines. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 832–836, 2013.
Application of high throughput pretreatment and co‐hydrolysis system to thermochemical pretreatment. Part 2: Dilute alkali
Authors: Li Hongjia , Gao Xiadi , DeMartini Jaclyn D. , Kumar Rajeev , Wyman Charles E. 
Published: May 2013

ABSTRACTHigh throughput pretreatment (HTPH) and enzymatic hydrolysis systems are now vital for screening large numbers of biomass samples to investigate biomass recalcitrance over various pretreatment and enzymatic hydrolysis conditions. Although hydrothermal pretreatment is currently being employed in most high throughput applications, thermochemical pretreatment at low and high pH conditions can offer additional insights to better understand the roles of hemicellulose and lignin, respectively, in defining biomass recalcitrance. Thus, after successfully applying the HTPH approach to dilute acid pretreatment [Gao et al. (2012) Biotechnol. Bioeng. 110(3): 754–762], extension to dilute alkali pretreatment was also achieved using a similar single‐step neutralization and buffering concept. In the latter approach, poplar and switchgrass were pretreated with 1 wt% sodium hydroxide at 120°C for different reaction times. Following pretreatment, an H2Cit−/HCit2− buffer with a pH of 4.5 was used to condition the pretreatment slurry to a pH range of 4.69–4.89, followed by enzymatic hydrolysis for 72 h of the entire mixture. Sugar yields showed different trends for poplar and switchgrass with increases in pretreatment times, demonstrating the method provided a clearly discernible screening tool at alkali conditions. This method was then applied to selected Populus tremuloides samples to follow ring‐by‐ring sugar release patterns. Observed variations were compared to results from hydrothermal pretreatments, providing new insights in understanding the influence of biomass structural differences on recalcitrance. Biotechnol. Bioeng. 2013;110: 2894–2901. © 2013 Wiley Periodicals, Inc.
A tandem laboratory scale protein purification process using Protein A affinity and anion exchange chromatography operated in a weak partitioning mode
Authors: Shamashkin Michael , Godavarti Ranga , Iskra Timothy , Coffman Jon 
Published: May 2013

ABSTRACTA significant consequence of scaling up production of high titer monoclonal antibody (mAb) processes in existing facilities is the generation of in‐process pools that exceed the capacity of storage vessels. A semi‐continuous downstream process where columns and filters are linked and operated in tandem would eliminate the need for intermediate holding tanks. This study is a bench‐scale demonstration of the feasibility of a tandem process for the purification of mAbs employing an affinity Protein A capture step, followed by a flow‐through anion‐exchange (AEX) step with the possibility of adding an in‐line virus filtration step (VF). All three steps were linked sequentially and operated as one continuous process using an ÄKTA FPLC equipped with two pumps and a system of valves and bypasses that allowed the components to be engaged at different stages of the process. The AEX column was operated in a weak partitioning (WP) mode enabled by a precise in‐line titration of Protein A effluent. In order to avoid complex control schemes and facilitate validation, quality and robustness were built into the system through selection of buffers based on thermodynamic and empirical models. The tandem system utilized the simplest possible combination of valves, pumps, controls, and automation, so that it could easily be implemented in a clinical or commercial production facility. Linking the purification steps in a tandem process is expected to generate savings in time and production costs and also reduce the size of quality systems due to reduced documentation requirements, microbial sampling, and elimination of hold time validation. Biotechnol. Bioeng. 2013;110: 2655–2663. © 2013 Wiley Periodicals, Inc.
Reliable and sensitive determination of dutasteride in human plasma by liquid chromatography–tandem mass spectrometry
Authors: Contractor Pritesh , Kurani Hemal , Guttikar Swati , Shrivastav Pranav S. 
Published: May 2013

ABSTRACTAn accurate and precise method was developed and validated using LC‐MS/MS to quantify dutasteride in human plasma. The analyte and dutasteride‐13C6 as internal standard (IS) were extracted from 300 μL plasma volume using methyl tert‐butyl ether–n‐hexane (80:20, v/v). Chromatographic analysis was performed on a Gemini C18 (150 × 4.6 mm, 5 µm) column using acetonitrile–5 mm ammonium formate, pH adjusted to 4.0 with formic acid (85:15, v/v) as the mobile phase. Tandem mass spectrometry in positive ionization mode was used to quantify dutasteride by multiple reaction monitoring. The entire data processing was done using Watson LIMSTM software, which provided excellent data integrity and high throughput with improved operational efficiency. The calibration curve was linear in the range of 0.1–25 ng/mL, with intra‐and inter‐batch values for accuracy and precision (coefficient of variation) ranging from 95.8 to 104.0 and from 0.7 to 5.3%, respectively. The mean overall recovery across quality controls was ≥95% for the analyte and IS, while the interference of matrix expressed as IS‐normalized matrix factors ranged from 1.01 to 1.02. The method was successfully applied to support a bioequivalence study of 0.5 mg dutasteride capsules in 24 healthy subjects. Assay reproducibility was demonstrated by reanalysis of 103 incurred samples. Copyright © 2013 John Wiley & Sons, Ltd.
Coping with Age‐related Threats to Role Identity: Older Couples and the Management of Household Money
Authors: Bisdee Dinah , Price Debora , Daly Tom 
Published: May 2013

ABSTRACTAs more couples live together into old age, difficult decisions have to be made about money matters, including the financing of late‐life care. This paper analyses in‐depth qualitative data from six older heterosexual couples, part of a wider study concerning money management in later life. Research when these cohorts were younger found that the organisation of money management within households was specialised and highly gendered, leading to substantive imbalances of power and access to financial resources, while also being core to the formation and maintenance of gendered role identities and couple identities. We find in this study that if a partner's ability to fulfil a money management role identity is threatened by later‐life issues such as poor health and cognitive decline, the other partner may try to protect that aspect of the spouse's role identity, using various covert strategies. This might be done to shore up the spouse's self‐esteem in the face of such age‐related threats to role identity, to ‘keep up appearances’ to the outside world or to maintain their identity as a couple at a time of life when there may be multiple difficulties to deal with. These findings have implications for practice and policy in the realm of money and identity management in later life. Copyright © 2013 John Wiley & Sons, Ltd.
On the Nature of Selective Palladium‐Based Nanoparticles on Nitrogen‐Doped Carbon Nanotubes for the Direct Synthesis of H2O2
Authors: Abate S. , Freni M. , Arrigo R. , Schuster M. E. , Perathoner S. , Centi G. 
Published: May 2013

AbstractCatalysts based on Pd and Pd–Au nanoparticles supported on N‐doped carbon nanotubes (N‐CNTs) are studied in the direct synthesis of H2O2. The initial selectivity in H2O2 formation is rather high (>95 %); however, there is a fast initial decrease during the first hour of time on stream. This was due to the initial presence of an organic capping agent (polyvinyl alcohol, which is used in the catalyst synthesis to obtain a high dispersion of metal particles). The removal of this capping agent during the reaction leads to a high mobility of metal nanoparticles. The high initial selectivity, when the capping agent is present, is due to small Pd terraces fully covered with chemisorbed O2 and limited H2 chemisorbed sites that consecutively hydrogenate the formed H2O2. The alloying of Pd with Au decreases the intrinsic reaction rate (per mg of Pd) and increases the selectivity in H2O2 formation, whereas Au alone is inactive. Au also has a minor effect on the consecutive conversion of H2O2 in both the decomposition and hydrogenolysis (in the presence of H2 only) reactions. These results suggest that Au does not block the unselective sites of H2O2 conversion but mainly creates isolated small terraces of Pd that can limit H2 chemisorption sites, which thus leads to higher selectivity to H2O2 under given reaction conditions.
ChemInform Abstract: Phosphide Oxides RE2AuP2O (RE: La, Ce, Pr, Nd): Synthesis, Structure, Chemical Bonding, Magnetism, and 31P and 139La Solid State NMR.
Authors: Bartsch Timo , Wiegand Thomas , Ren Jinjun , Eckert Hellmut , Johrendt Dirk , Niehaus Oliver , Eul Matthias , Poettgen Rainer 
Published: May 2013

AbstractThe title compounds are prepared by solid state reaction of the rare earth metals, their appropriate oxides, finely dispersed gold, and red phosphorus (sealed silica tube, 1173 K, 72 h).
ChemInform Abstract: Y3MnAu5: Three Distinctive d‐Metal Functions in an Intergrown Cluster Phase.
Authors: Samal Saroj L. , Pandey Abhishek , Johnston David C. , Corbett John D. 
Published: May 2013

AbstractThe new intermetallic phase of title is prepared in virtually quantitative yield by arc‐melting of the elements followed by annealing at 1000 °C for about 3 d.
ChemInform Abstract: An Efficient Synthesis of Optically Active Trifluoromethyl Aldimines via Asymmetric Biomimetic Transamination.
Authors: Liu Mao , Li Jing , Xiao Xiao , Xie Ying , Shi Yian 
Published: May 2013

AbstractA quinine derivative is used as an efficient chiral catalyst for the asymmetric synthesis of trifluoromethyl aldimines (II) by [1,3]‐proton shift of imines (I).
ChemInform Abstract: Stereoselective Rhodium‐Catalyzed [3 + 2 + 1] Carbocyclization of Alkenylidenecyclopropanes with Carbon Monoxide: Theoretical Evidence for a Trimethylenemethane Metallacycle Intermediate.
Authors: Mazumder Shivnath , Shang Deju , Negru Daniela E. , Baik Mu‐Hyun , Evans P. Andrew 
Published: May 2013

AbstractThe presented new carbocyclization of carbon‐ and heteroatom‐tethered alkenylidenecyclopropanes with CO results in the stereoselective construction of cis‐fused bicyclohexenones.
ChemInform Abstract: Gluconic Acid Aqueous Solution: A Task‐Specific Bio‐Based Solvent for Ring‐Opening Reactions of Dihydropyrans.
Authors: Yang Jie , Zhou Binghua , Li Minghao , Gu Yanlong 
Published: May 2013

AbstractA variety of dihydropyran ring opening reactions using an aqueous gluconic acid solution as reaction medium is studied.
ChemInform Abstract: Oxidation of Benzyl Alcohols by Semi‐Stoichiometric Amounts of Cobalt‐Doped Birnessite‐Type Layered MnO2 under Oxygen Atmosphere.
Authors: Kamimura Akio , Nozaki Yuichiro , Nishiyama Mai , Nakayama Masaharu 
Published: May 2013

AbstractThe title reaction takes place selectively with benzylic alcohols (I), whereas allylic alcohols such as (III) are oxidized quite slowly.
ChemInform Abstract: Asymmetric Hydrogenation of α,α′‐Disubstituted Cycloketones Through Dynamic Kinetic Resolution: An Efficient Construction of Chiral Diols with Three Contiguous Stereocenters.
Authors: Liu Chong , Xie Jian‐Hua , Li Ya‐Li , Chen Ji‐Qiang , Zhou Qi‐Lin 
Published: May 2013

AbstractThis method uses catalyst loadings as low as 0.1 mol% and yields (cis,cis)‐reaction products in good yields and with high enantio‐ and diastereoselectivities.
ChemInform Abstract: Solvent‐Free Catalytic Preparation of 1,1‐Diacetates Using a Silica‐Supported Functional Ionic Liquid as Catalyst.
Authors: Kang Li Q. , Cai Yue Q. , Cheng Lin 
Published: May 2013

AbstractAromatic diacetates (III) are prepared under solvent‐free conditions from the corresponding aldehydes using a silica‐supported ionic liquid as catalyst.
ChemInform Abstract: C(sp3)—C(sp3) and C(sp3)—H Bond Activation of 1,1‐Disubstituted Cyclopentane.
Authors: Mukai Chisato , Ohta Yuu , Oura Yuki , Kawaguchi Yasuaki , Inagaki Fuyuhiko 
Published: May 2013

AbstractThis method includes an unprecedented C—C bond cleavage of an unactivated cyclopentane ring.
ChemInform Abstract: Efficient Preparation of Biologically Important 1,2‐Amino Alcohols.
Authors: Gupta Pankaj , Rouf Abdul , Shah Bhahwal A. , Mukherjee Debaraj , Taneja Subhash C. 
Published: May 2013

AbstractAn efficient three‐step methodology for the formation of 1,2‐amino alcohols (V) is developed which involves a rapid coupling between bromoketones and potassium phthalimide, subsequent selective reduction and finally an efficient removal step of the phthalimido protection group.
ChemInform Abstract: Chemistry of Dimedone for Synthesis of Oxygen‐, Nitrogen‐, and Sulfur‐Containing Heterocycles from 2‐(3‐Hydroxy‐5,5‐dimethylcyclohex‐2‐enylidene)malononitrile.
Authors: Majumdar Poulomi , Mohanta Prajna Parimita , Behera Rajani K. , Behera Ajaya Kumar 
Published: May 2013

AbstractAn efficient route for the synthesis of a wide range of new O‐, N‐, and S‐containing spiro heterocycles via reactions of readily available malononitrile (III) with active methylene or bidentate compounds is presented.
ChemInform Abstract: Electrophile‐Induced C—C Bond Activation of Vinylcyclopropanes for the Synthesis of Z‐Alkylidenetetrahydrofurans.
Authors: Ganesh Venkataraman , Kundu Taraknath , Chandrasekaran Srinivasan 
Published: May 2013

AbstractThe reaction is stereospecific and proceeds via bromenium‐catalyzed C—C bond activation of vinyl cyclopropanes and the following cyclization to alkylidenetetrahydrofuran and other heterocycles.