bluuebunny/crossref_metadata_2025_split huggingface dataset:
Granulomatous Tissue Reaction and Calcification of Heterologous Elastin Implanted in Hairless Mouse Skin: Light and Electron Microscopic Studies Compared with Reaction of Heterologous Collagen
Authors: Tsuji Takuo , Sawabe Motokazu 
Published: April 2015

AbstractHeterologous elastin particles were injected into the deep dermis or below the panniculus carnosus of hairless mice. A granulomatous tissue reaction with concomitant giant cell formation occurred around the implanted elastin in the early stages and, subsequently, calcium deposition with disappearance of the histiocytes and giant cells occurred in the later stages. Ultrastructural study revealed that some elastin particles were engulfed by the giant cells; others underwent calcification but remained intact.The heterologous collagen used as a control also induced a granulomatous tissue reaction, but did not undergo calcification and gradually disappeared.These results suggest that the heterologous elastin and collagen particles exhibit different susceptibilities to digestion. However, it is not clear why the heterologous elastin underwent calcification.
Properties of γ‐Glutamyl Transpeptidase in Squamous Cell Carcinoma
Authors: Takami Yoshihiro , Chiba Masahito , Takahashi Makoto 
Published: April 2015

Abstractγ‐Glutamyl transpeptidase (GGT) was extracted from squamous cell carcinoma tissues of human skin (SCC) by Triton X‐100 and bromelain treatment, and some of its biochemical properties were compared with those of GGT extracted from eccrine gland‐rich tissue and normal kidney. GGT activity significantly increased in SCC, but there was no definitive differences in enzymological properties between GGT of SCC and normal tissue enzyme. However, GGT of SCC was distinguishable from those of normal tissues by isoelectric point, electrophoretic mobility, and sensitivity to neuraminidase treatment. These results indicate that GGT of SCC has some variant properties which may be related to skin carcinogenesis.
A Study of the Efficacy of Antimicrobial Detergents for Hand Washing: Using the Full‐hand Touch Plates Method
Authors: Namura Shoko , Nishijima Setsuko , McGinley Kenneth J. , Leyden James J. 
Published: April 2015

AbstractWe studied the effects of four kinds of antimicrobial detergents, 4% chlorhexidine gluconate (CHG), chlorbenzarconium (CBC), 10% povidone‐iodine (PVI), 0.3% triclosan (TRI), and one non‐medicated detergent (NMD) using the full‐hand touch plates method.Before and after 3 minutes of hand scrubbing with a brush, bacterial colonies on the hand surface and subungual bacteria were counted. CHG, CBC, and PVI were excellent antimicrobial detergents against hand surface bacteria, but most of the subungual bacteria remained. The subungual space was the most difficult region from which to eradicate bacteria. TRI was much less effective than hand soap against hand surface bacteria.
Self Limited Dermal Invasion of Keratinocytes in Maculopapular Eruptions after Systemic Chemotherapy
Authors: Hann Seung Kyung , Rhoe Byung Seon , Kang Won Hyoung , Park Yoon‐Kee 
Published: April 2015

AbstractBenign, multifocal, and transient (spontaneously disappearing within one month) stromal invasions of atypical basal keratinocytes were found in light and electron microscopic examination of maculopapular eruptions which developed after receiving an 8 day cycle of doxorubicin, 5‐fluorouracil (5‐FU), and cyclophosphamide (Cy) in a 63‐year‐old woman. She had had a modified radical mastectomy to remove a carcinoma of her right breast.
Concomitant Occurrence of Circulating IgA Anti‐intercellular and Anti‐basement Membrane Zone Antibodies in Autoimmune Blistering Diseases: Immunofluorescence and Immunoblot Studies
Authors: Dmochowski Marian , Hashimoto Takashi , Bhogal Balbir S. , Black Martin M. , Miyakawa Kaori , Miyamoto Ryoko , Nishikawa Takeji 
Published: April 2015

AbstractRecently, cases with circulating IgA anti‐intercellular antibodies have been described. The objective of this study was to present immunofluorescence and immunoblot findings in three cases of bullous diseases with concomitant circulating IgA anti‐intercellular and anti‐basement membrane zone antibodies. Direct immunofluorescence, indirect immunofluorescence on intact and 1M NaCl‐split skin, immunoblotting of epidermal extracts from dispase‐ and EDTA‐separated (two different procedures) human skin, and immunoblotting of the bovine desmosome preparation were performed. All three cases had IgA anti‐intercellular and anti‐basement membrane zone antibodies. However, immunoblot results were divergent. Case 1 had antibodies against the 150 kD pemphigus foliaceus antigen (IgG), the 170 kD protein (IgG and IgA), and the 97 kD antigen (IgG and IgA). Case 2 had IgG antibodies reactive with the 230 kD and the 170 kD bullous pemphigoid antigens, while case 3 had IgA antibodies against the 97 kD antigen only. The results of immunofluorescence and immunoblot studies in our patients widen the spectrum of laboratory features in blistering skin diseases mediated, at least in part, by antibodies of the IgA class.
Epidermolysis Bullosa Acquisita (EBA) with Nonclassical Distribution of Eruptions
Authors: Matsumura Yasuhiro , Hamanaka Hiroshi , Horiguchi Yuji , Tanaka Toshihiro , Okuwa Takashi , Imamura Sadao 
Published: April 2015

AbstractThe skin lesions in epidermolysis bullosa acquisita (EBA), a mechanobullous disease, often show acral distribution. Recently, we experienced a case of EBA in which most of the skin lesions were located on the trunk. We reviewed the distribution of the skin eruptions in 58 reported cases of EBA. Although the extensor surfaces of the extremities are the most common site, there were some cases with non‐acral distribution. These “nonclassical” cases should also be considered in the clinical diagnosis of EBA and other bullous diseases.
Sexually Transmitted Diseases in a HIV Infected Patient
Authors: Ishii Norihisa , Ichiyama Shin‐ichi , Nakajima Hiroshi , Ito Akira 
Published: April 2015

AbstractWe report a case of HIV infection with herpes simplex, syphilis, and condyloma acuminatum in a 66‐year‐old heterosexual man. The patient was an asymptomatic carrier (group II) of HIV infection. Herpes simplex on his penis was controlled by aciclovir. Secondary syphilis was treated with penicillin G, after which skin lesions disappeared. These four diseases were all sexually transmitted diseases (STD).One should consider the possible existence of other STD in similar cases.
Incidental Epidermolytic Hyperkeratosis in Progressive Systemic Sclerosis
Authors: Sasaki Tetsuo , Nakajima Hiroshi 
Published: April 2015

AbstractIncidental epidermolytic hyperkeratosis (EH) has been reported in a variety of lesions. We describe here incidental EH in a patient with progressive systemic sclerosis (PSS). We reviewed 108 skin biopsies from our cases of PSS and localized scleroderma, but this finding was seen only in the present case. Focal acantholytic dyskeratosis is analogous to EH, but we believe that this case is most consonant with EH. Since there has been no other report of EH in PSS, this association may be coincidental.
Prominent Telangiectasia Associated with Marked Bleeding in CREST Syndrome
Authors: Ueda Masumi , Abe Yoshiko , Fujiwara Hideki , Fujimoto Wataru , Arakawa Kenzo , Arata Jirô , Yoshioka Toshifumi , Tomoda Jun , Katayama Haruko 
Published: April 2015

AbstractA 64‐year‐old woman with CREST syndrome developed prominent telangiectases mimicking hereditary hemorrhagic telangiectasia (HHT) of Osler‐Rendu‐Weber. We have been following her since she first came to us with discrete telangiectatic mats and Raynaud's phenomenon 11 years ago. Telangiectatic lesions have been seen on her larynx and esophagus in addition to commonly affected sites. She has experienced spontaneous epistaxis and marked bleeding from the lesions on her lips, oral mucous membrane, and soles.This case illuminates new aspects of telangiectasia in CREST syndrome.
Eosinophilic Panniculitis
Authors: Kato Naoko 
Published: April 2015

AbstractA 39‐year‐old obese female patient with borderline diabetes mellitus who developed multiple, tender, and hard nodules on the lower abdominal wall is described. The histological features were characterized by mixed panniculitis, showing massive eosinophilic infiltration and fibrosis in the septae and many foam cells in the parenchymal area of subcutaneous tissues. The eruption regressed gradually during the course of diet therapy for diabetes mellitus and obesity. Although the etiology is diverse and nonspecific, eosinophilic panniculitis seems to be a unique histopathological reaction pattern characterized by massive eosinophilic infiltration into subcutaneous tissues.
Furuncle‐like Lesions in Mouse Experimental Skin Infections with Staphylococcus aureus
Authors: Abe Yoshiko , Akiyama Hisanori , Arata Jirô 
Published: April 2015

AbstractThe pathomechanism of furuncle has not been fully elucidated and should be investigated using an appropriate animal model. We observed the invasion of Staphylococcus aureus cells into hair follicles in mice, using a strain of S. aureus isolated from human furunculosis. Light microscopical examination revealed that S. aureus cells attached to corneocytes at 6 h after inoculation, proliferated around the ostium of the hair follicle and invaded the hair follicle at 12 h after inoculation. Electron microscopically, S. aureus cells attached to the horny layer of hair follicle with long, thick, string‐like structures. At 12 h after inoculation, S. aureus cells invaded in a file between the inner root sheath and outer root sheath. We could not induce direct invasion from the follicle ostium. Our findings suggest that there are some regions of the hair follicle through which S. aureus cells can relatively easily invade deeper into the follicle. The most important question is what confines the invasion and inflammation of S. aureus to the hair follicle. We suggest that there is some locus minoris for invasion into hair follicles by S. aureus, such as an interface between the two sheaths.
Light Microscopic Autoradiographical Analysis of [125I]Epidermal Growth Factor Binding in Basal Cell Epithelioma and Squamous Cell Carcinoma of the Skin
Authors: Ikeuchi Tsuneo , Urano Yoshio , Fukuhara Kosaku , Nakanishi Hideki , Arase Seiji 
Published: April 2015

AbstractLevels of epidermal growth factor (EGF) receptor expression were investigated in five basal cell epitheliomas (BCEs) and 10 primary lesions from squamous cell carcinoma (SCC) of the skin, using light microscopic autoradiography with [125I]EGF. All of the BCEs were clinically the pigmented type and histologically the solid type. All of them showed an EGF binding level similar to that of the basal and suprabasal layers of the normal epidermis. The SCCs included one case of Bowen's disease (SCC in situ), five of the well differentiated type, three of the moderately differentiated type and one of the poorly differentiated type. Eight of the 10 SCCs showed an EGF binding level similar to that of the normal epidermal basal and suprabasal cell layers. One of the two remaining SCCs, a moderately differentiated type, showed highly increased EGF binding. The other one, a poorly differentiated type, showed very little EGF binding in a large region consisting of poorly differentiated cells, although a small area composed of more differentiated, nest‐forming cells had an EGF binding level similar to that of the basal and suprabasal cells. Metastasis was found in three of these 10 SCCs after surgery. Two of the three SCCs with metastasis showed increased or decreased EGF binding levels in primary lesions as described above; in contrast, primary lesions of the seven SCCs without metastasis had EGF binding levels similar to those of the normal epidermal basal and suprabasal cells. Abnormally increased or decreased EGF binding level in SCC of the skin may be indicative of a poor prognosis, although it is necessary to examine more SCCs to confirm this assumption.
Giant Mucocele of Oral Cavity as a Mucocutaneous Manifestation of Sjögren Syndrome
Authors: Katayama Ichiro , Yamazaki Sayuri , Nishioka Kiyoshi 
Published: April 2015

AbstractTwo cases of Sjögren syndrome who developed a giant mucocele on the floor of the oral cavity are reported. Histological analysis revealed the dense infiltration of lymph‐plasmacytic infiltration around the dilated salivary duct. Similar findings were observed in the biopsied specimens of the labial salivary gland which were consistent with the grade 4 score proposed by Chisholm and Mason's criteria. Mucocele is a common mucocutaneous disease of unknown etiology except for traumatic origin and Sjögren syndrome might be one of the important underlying disease which causes giant mucocele, especially in elderly female patients.
Effects of Krestin (PSK) on Tumorigenesis Induced by Two‐stage and Complete Chemical Carcinogenesis
Authors: Nakayama Juichiro , Urabe Atsumichi , Terao Hiroshi , Moon Doo Chan , Hori Yoshiaki , Kinoshita Nadao 
Published: April 2015

AbstractThe effects of PSK on tumorigenesis in mouse skin were investigated either when mouse skins were initiated by benzo(a)pyrene and promoted by 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (Group I, two‐stage carcinogenesis) or when both initiated and promoted by benzo(a)pyrene (Group II, complete carcinogenesis). Twelve mice in each group were fed chow with or without 0.4% PSK. This concentration of PSK was determined by calculation to give mice enough PSK to exert antitumorigenic activity without cytotoxicity. By the end of the experimental periods (26 weeks), two carcinoma‐burdened mice in Group I without PSK were dead, but no carcinomas at all were identified in the mice fed with PSK, although considerable numbers of papillomas developed in both groups. In Group II, carcinomas started to evolve at the 15th week of the experiment regardless of PSK feeding. The number of carcinomas observed in the mice fed with PSK in Group II was statistically significantly lower than that in the mice fed without PSK. Histologically, mild inflammatory infiltrations were seen around the papillomas, and moderate to dense infiltrations, mainly composed of neutrophils, T lymphocytes, and macrophages, were observed in squamous cell carcinomas. There were apparently no significant differences in the number of the infiltrating cells around carcinomas in PSK (+) and PSK (−) groups in both early and fully developed lesions. However, considerable numbers of cells infiltrating into the nests were observed in the early lesions of elicited carcinomas in the mice fed with PSK, while such cells were rarely seen in carcinoma nests in the group without PSK at that stage. The multi‐stage carcinogenesis regimen which evokes both benign and malignant epidermal tumors in mouse skin should provide insights into the function of the specific infiltration of immuno‐competent cells and should also be a valid system for the quantitative and qualitative analysis of the anti‐tumor effects of immunopotentiators such as PSK.
Immunohistochemical Study of Graft‐versus‐Host Reaction (GVHR)‐Type Drug Eruptions
Authors: Osawa Junko , Kitamura Kazuko , Saito Sumi , Ikezawa Zenro , Nakajima Hiroshi 
Published: April 2015

AbstractSkin biopsies of graft‐versus‐host reaction (GVHR)‐type drug eruptions in the acute phase were compared immunohistochemically with those in the chronic phase and also with non‐GVHR type drug eruptions in the acute phase. Predominance of CD8+ T cells in the epidermal infiltrates, reduction in the number of epidermal OKT6+ dendritic cells (Langerhans cells), and increased expression of HLA‐DR and ICAM‐1 on keratinocytes were observed in the acute phase of GVHR‐type, but not in either the chronic phase of GVHR‐type or the acute non‐GVHR type. These findings were similar to those of previous reports on skin lesions of acute GVH disease (GVHD) seen after bone marrow transplantation.Therefore, immunohistochemistry is not useful for differential diagnosis between acute GVHR‐type drug eruptions and acute cutaneous GVHD. These findings also indicate that similar immunomechanisms may be involved in the pathogenesis of both GVHR‐type drug eruptions and cutaneous GVHD.
Scrofuloderma of the Scalp, Psoas Abscess, and Caries Spine: An Unusual Association
Authors: Sehgal Virendra N. , Chander Ram , Garg Vijay K. , Karmakar Sanchita 
Published: April 2015

AbstractAn unusual association of scrofuloderma of the scalp, psoas abscess, and caries spine was seen in a 4‐year‐old girl. The diagnosis was confirmed by relative and absolute criteria. The peripheral T‐lymphocytes and their subsets were within normal limits, although her immunohistopathological changes indicated an intermediary response across the spectrum depicting an unstable immunity. This may have been responsible for the silent spread of Mycobacterium tuberculosis and the current association.
Rudimentary Polydactyly
Authors: Chung Joon , Nam In Whan , Ahn Sung Ku , Lee Seung Hun , Kim Joong Gie , Sung Yeol Oh 
Published: April 2015

AbstractA case of rudimentary polydactyly on the radial aspect of the thumb of a 4‐year‐old male was examined. Histologic examination revealed hyperkeratosis and acanthosis in the epidermis and large numbers of nerve bundles in the dermis. With immunoperoxidase staining for S‐100 protein, the nerve corpuscles were positively stained. We think that rudimentary polydactyly is a kind of congenital traumatic neuroma and that the possible cause may be intrauterine amputation.
Pemphigus Vulgaris Associated with Autoimmune Hemolytic Anemia and Elevated TNFα
Authors: Ujihara Mayumi , Hamanaka Sumiko , Matsuda Sachie , Numa Fumitaka , Kato Hiroshi 
Published: April 2015

AbstractA 76‐year‐old female was admitted with many bullae and erythema on her trunk and extremities. A biopsy specimen showed significant intercellular edema in the lower epidermis and eosinophilic infiltration into the dermis and the epidermis. Immunofluorescent staining revealed the deposition of IgG in the intercellular area of her prickle cells. From these histologic findings and the typical clinical features, we diagnosed her as having pemphigus vulgaris. Examination of her blood revealed that she also suffered from autoimmune hemolytic anemia. Despite intensive treatment with prednisolone, she finally died. This case is of interest because of its rarity and the TNFα detected significantly in the blister fluid of this patient.
Biochemical Analyses of Macromolecular Matrix Components in Patients with Pseudoxanthoma Elasticum
Authors: Sakuraoka Koichi , Tajima Shingo , Nishikawa Takeji , Seyama Yoshiyuki 
Published: April 2015

AbstractTwo cases of pseudoxanthoma elasticum (PXE) were reported. Biochemical studies of collagen, glycosaminoglycan, and elastin content were performed using skin specimens from affected lesions. Hydroxyproline content was reduced approximately 46% and 24% in cases 1 and 2, respectively. The reduction in collagen content resulted from the reduction in the lower dermis, not from that in the upper dermis. Glycosaminoglycan content in PXE was also decreased, with a slight increase in the ratio of hyaluronic acid to dermatan sulfate. Isodesmosine content in PXE was increased 3.2‐ and 2.2‐fold in cases 1 and 2, respectively. These results suggest that the major and primary biochemical changes in PXE are an increase in elastin content.
Mucinous Carcinoma of the Skin: Report of a Case with DNA Cytofluorometric Study
Authors: Katoh Norito , Hirano Shinya , Hosokawa Youhei , Miyashita Aya , Kishimoto Saburo , Yasuno Hirokazu 
Published: April 2015

AbstractA 62‐year‐old man with a mucinous carcinoma of the skin on the left cheek is described. The excised specimen showed a characteristic histopathologic picture. The findings from histochemical, immuno‐histochemical, and electron microscopic examinations suggested an eccrine differentiation of the tumor cells. The removed tumor was examined using DNA cytofluorometry in order to analyze the cell kinetics and to estimate its biological behavior and prognosis. The tumor cells showed a diploid mode with a small number of polyploid cells. Only a small number of cells in the S and G2/M phase ranges were detected. After four years, there has been no evidence of tumor recurrence nor metastasis. The results of DNA ploidy analysis were thus compatible with the nature of slow growth with a good prognosis in this case.
Black Dot Ringworm: The First Report of a Patient from the San'in District
Authors: Dekio Satoshi , Jidoi Joji , Yamada Yoshitaka 
Published: April 2015

AbstractA 77‐year‐old Japanese woman, a resident of Shimane Prefecture, with black dot ringworm caused by a Trichophyton glabrum infection on the scalp is described. To the best of our knowledge, she is the first case of BDR reported from the San'in District.
Mycosis Fungoides with Leser‐Trélat Sign: The First Report of a Patient from Japan
Authors: Miyako Fukami , Dekio Satoshi , Tamura Haruko , Yamada Yoshitaka , Miyata Nobuko , Jidoi Joji , Tohgi Kimiko 
Published: April 2015

AbstractWe describe here a 35‐year‐old Japanese woman with mycosis fungoides (MF) who developed numerous, rapidly‐growing, seborrheic keratoses on the front of her neck and chest. To the best of our knowledge, this is the first reported case of MF with Leser‐Trélat sign from Japan.
Langerhans Cell Histiocytosis Presenting as a Varicelliform Eruption over the Entire Skin
Authors: Johno Masayoshi , Oishi Munashi , Kohmaru Masahiro , Yoshimura Kohji , Ono Tomomichi 
Published: April 2015

AbstractA boy with skin eruptions resembling varicella and specific for Langerhans cell histiocytosis (LCH) is reported. At his initial visit when he was four months old, vesiculopustular lesions were present over the entire body; these had first appeared on the third day post partus. Histopathological, immunohistochemical, and electron microscopical examination confirmed the Langerhans cell phenotype and Birbeck granules in the responsible cells. He also had hydronephrosis, recurrent fever, and cutaneous bacterial infections. His parents refused further medical treatment and he died of diarrhea with cachexia about two years later. LCH may present diagnostic difficulties by manifesting as a skin eruption which resembles varicella.
Production of Overmodified Type I Procollagen in a Case of Osteogenesis Imperfecta
Authors: Tajima Shingo , Takehana Makoto , Azuma Noriyuki 
Published: April 2015

AbstractCollagen synthesis in cultured skin fibroblasts from a patient with osteogenesis imperfecta was studied. Approximately 2 fold accumulation of collagen in the cell layer was found. The slower mobility of proα1 (I) and proα2 (I) as well as α1 and α2 (I) polypeptide on sodium dodecylsulfate‐polyacrylamide gel electrophoresis was detected, indicating that abnormal posttranslational modification could be present in type I procollagen in patient fibroblasts. The degrees of hydroxylation and subsequent glycosylation of lysine residues in the affected collagen were elevated 1.5 and 1.4 fold, respectively. There were no significant changes in the relative content of type III to type I collagen nor the incorporation of mannose into the carboxyterminal propeptide of proα1 (I) and proα2 (I). These results indicate that the patient produces an over‐modified type I procollagen which is responsible for the clinical features and has a collagen abnormality already reported in type II osteogenesis imperfecta.