bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Mini-Fyke Nets for Sampling Shallow-Water Fish Communities in Florida Lakes
Authors: Bonvechio K. I. , Sawyers R. E. , Bitz R. , Crawford S. 
Published: June 2014

Abstract
We evaluated mini-fyke nets for sampling shore-based (SB; &lt;1 m deep) fish communities in Florida lakes. Specifically, we wanted to determine the most effective deployment method and sample size required for adequately characterizing fish communities in these habitats. Shallow SB (0.15–0.6 m) net sets, in which nets were not completely submersed, collected significantly more individuals and a larger proportion of poeciliids. However, deep SB (0.6–1.0 m) net sets, in which nets were completely submersed, collected a larger proportion of centrarchids, cyprinids, and cyprinidontids. Net placement also significantly affected the overall catch and composition of samples. Nets placed in locations away from the lake boundary tended to collect actively schooling fishes such as Threadfin Shad Dorosoma petenense and cyprinids. Nets placed next to the lake boundary tended to collect species associated with shallow, vegetated habitats, particularly poeciliids, at greater frequencies. In some cases, the collection of these fishes was unique to a particular deployment method. Our results suggest that in long-term monitoring of Florida's shore-based lentic fish communities, nets should be placed in SB waters with the lead extending to the lake boundary. Although long-term sampling objectives may vary, we determined that a sampling target of 30 net sets was sufficient for characterizing the fish community in terms of percentage composition, species richness, and species diversity. Furthermore, we recommend the continued use of additional gears to fully characterize littoral fish communities.
Received December 19, 2013; accepted March 1, 2014
Estimated Prevalence of Tuberculosis Infection Among a New York City Clinic Population Using Interferon-gamma Release Assays
Authors: Stennis Natalie L. , Trieu Lisa , Ahuja Shama D. , Harris Tiffany G. 
Published: June 2014

Abstract
Background.  Elimination of tuberculosis (TB) in the United States requires treating not only persons with active disease but also those infected with TB. Achieving this goal requires understanding local TB infection prevalence and identifying subgroups at increased risk for infection and disease.
Methods.  The study population included all patients tested with an interferon-gamma release assay (IGRA) test at New York City (NYC) public TB clinics from October 1, 2006 to December 31, 2011. Patients who were not a case or contact at testing (general clinic patients) and who had a positive QuantiFERON-Gold In-Tube (QFT-GIT) test result were compared with those with indeterminate or negative results to identify characteristics associated with positive results. New York City TB surveillance data were used to identify clinic patients later diagnosed with active TB disease.
Results.  A total of 69 273 IGRA tests were conducted. Among 20 066 patients tested with QFT-GIT, 16% tested positive, 83% tested negative, and &lt;1% were indeterminate. Of 18 481 general clinic patients, 14% had a positive QFT-GIT result. Nine percent of United States-born patients compared with 19% of foreign-born patients had a positive result. Increasing age and birth in a high-incidence country were associated with a higher likelihood of having a positive result. One patient with a negative QFT-GIT result was identified as a TB case 2 years later.
Conclusions.  Using QFT-GIT data, the background prevalence of TB infection in NYC was estimated. Patient characteristics associated with a positive QFT-GIT result were consistent with known TB risk factors. Results suggest that IGRAs are reliable tests for TB infection.
Addition of Glucose Oxidase for the Improvement of Refrigerated Dough Quality
Authors: Whitney Kristin , Ohm Jae‐Bom , Simsek Senay 
Published: June 2014

ABSTRACTRefrigerated dough encompasses a wide range of products and is a popular choice for consumers. Two of the largest problems that occur during refrigerated dough storage are dough syruping and loss of dough strength. The goal of this study was to evaluate glucose oxidase as an additive to refrigerated dough with the purpose of maintaining dough strength and retarding dough syruping. Refrigerated dough was evaluated for the degree of dough syruping (DDS), dough strength, rheological characteristics, baking quality, and protein quality. The addition of glucose oxidase at 10 mg/kg was able to significantly (P < 0.05) reduce dough syruping and maintain the strength of the dough. Addition of glucose oxidase at 5 and 25 mg/kg was not able to reduce the level of dough syruping at a satisfactory level. Degradation of protein was found to occur during storage of refrigerated dough. DDS had a negative correlation (r = –0.60 to –0.94) to the level of polymeric proteins and a positive correlation (r = 0.60 to 0.98) to the low‐molecular‐weight proteins. Overall, glucose oxidase at 10 mg/kg can improve refrigerated dough quality by reducing dough syruping and maintaining dough strength.
Effect of Transglutaminase, Citrate Buffer, and Temperature on a Soft Wheat Flour Dough System
Authors: Bagagli Marcela P. , Jazaeri Sahar , Bock Jayne E. , Seetharaman Koushik , Sato Helia H. 
Published: June 2014

ABSTRACTTransglutaminase (TGase) can improve the functional characteristics of proteins by introducing covalent bonds inter‐ or intrachains. Temperature and pH interfere with the protein structure and the catalytic activity of enzymes. Because these three factors can act synergistically, TGase, citrate buffer, and temperature were evaluated for their effects on the rheological and chemical changes in low‐protein wheat flour dough. Dough strength, measured by microextension test, significantly increased with increasing levels of TGase (8 U/g of protein), with changes in pH of the citrate buffer (pH 6.5), and by the effect of interaction between these factors. The same trend was observed in the size‐exclusion HPLC measurements, indicating that these two parameters have the effect of increasing gluten protein aggregation. Temperature had a significant effect on dough extension, measured by microextension test. The changes in secondary structure of gluten protein were investigated by FTIR second‐derivative spectra (amide I region, 1,600–1,700 cm−1) and showed an increase in β‐sheet structures initiated by TGase, citrate buffer pH, and their interaction.
Preparation of Synaptosomes from Postmortem Human Prefrontal Cortex
Authors: Smalheiser Neil R. , Lugli Giovanni 
Published: June 2014

AbstractSynaptosomes are a popular type of isolated synaptic fraction intensively used in neuroscience and cell biology. They are prepared by layering on density gradients and thought to consist largely of axonal endings with attached postsynaptic structures (Morgan, 1976), in contrast to synaptoneurosomes (Hollingsworth et al, 1985) which are prepared by filtration and are thought to consist largely of pinched-off dendritic spines with attached presynaptic structures. Although most studies of synaptosomes have utilized rodent or primate tissue, a score of studies have employed human samples derived from surgical specimens or postmortem brain. We recently described the isolation of synaptosomes from human postmortem prefrontal cortex to study the expression of synaptic microRNAs and other small RNAs in depression, schizophrenia and bipolar disorder (Smalheiser et al, 2014). This protocol alluded to methods and modifications that are scattered among several publications, and did not explain the reasons for the procedures chosen. Because our protocol differs from other published human synaptosome protocols in a variety of respects, we present here a detailed description of synaptosome preparation that should facilitate the use of this standardized synaptic fraction by other workers.
Inducible Repression of Nuclear-Encoded Subunits of the Cytochrome b6f Complex in Tobacco Reveals an Extraordinarily Long Lifetime of the Complex
Authors: Hojka Marta , Thiele Wolfram , Tóth Szilvia Z. , Lein Wolfgang , Bock Ralph , Schöttler Mark Aurel 
Published: June 2014

Abstract
The biogenesis of the cytochrome b6f complex in tobacco (Nicotiana tabacum) seems to be restricted to young leaves, suggesting a high lifetime of the complex. To directly determine its lifetime, we employed an ethanol-inducible RNA interference (RNAi) approach targeted against the essential nuclear-encoded Rieske protein (PetC) and the small M subunit (PetM), whose function in higher plants is unknown. Young expanding leaves of both PetM and PetC RNAi transformants bleached rapidly and developed necroses, while mature leaves, whose photosynthetic apparatus was fully assembled before RNAi induction, stayed green. In line with these phenotypes, cytochrome b6f complex accumulation and linear electron transport capacity were strongly repressed in young leaves of both RNAi transformants, showing that the M subunit is as essential for cytochrome b6f complex accumulation as the Rieske protein. In mature leaves, all photosynthetic parameters were indistinguishable from the wild type even after 14 d of induction. As RNAi repression of PetM and PetC was highly efficient in both young and mature leaves, these data indicate a lifetime of the cytochrome b6f complex of at least 1 week. The switch-off of cytochrome b6f complex biogenesis in mature leaves may represent part of the first dedicated step of the leaf senescence program.
Genetic structure of a hybrid zone between two violets, Viola rossii Hemsl. and V. bissetii Maxim.: dominance of F1 individuals in a narrow contact range
Authors: Nagano Yusuke , Hirao Akira S. , Itino Takao 
Published: June 2014

AbstractThe genetic composition of a hybrid zone can provide insight into the evolution of diversification in plants. We carried out morphological and amplified fragment length polymorphism analyses to investigate the genetic composition of a hybrid zone between two violets, Viola bissetii Hemsl. and Viola rossii Maxim. Our aim was to clarify the formation and maintenance of hybrids between these Viola species. We found that most hybrid individuals (V. bissetii × V. rossii) were of the F1 generation, with a few of the F2 generation. We found no backcrosses. The scarcity of post‐F1 hybrids indicates that a species barrier is established between the parental species. The F1‐dominated hybrid zone occupied only a narrow, intermediate ecotone between the parental habitats, suggesting that selection by environmental factors against hybrids may help to maintain the current conditions in this hybrid zone.
Natural Additives in Wheat‐Based Pasta and Noodle Products: Opportunities for Enhanced Nutritional and Functional Properties
Authors: Li Man , Zhu Ke‐Xue , Guo Xiao‐Na , Brijs Kristof , Zhou Hui‐Ming 
Published: June 2014

AbstractPasta products and noodles have been staple foods since ancient times in many countries all over the world. These cereal products are still increasingly popular worldwide for their convenience, nutritional properties, special flavor, and taste. Pasta and noodles are essentially the same type of food but differ in their raw materials and shaping process, as well as the people and regions in the world consuming them. Many additives have been developed and are being used today in pasta and noodle products for various purposes. However, due to lack of knowledge about specific uses for some additives, they are sometimes misused by manufacturers. This can lead to opposite technological effects instead and may even cause damage to human health. Due to consumer demands, interest now focuses on natural “green” food additives with broad‐spectrum functions, high effectiveness, and low toxicity. In order to provide detailed references for noodle and pasta production, as well as to provide ideas for developing new types of these products, here we summarize the types of natural additives that are being incorporated in pasta and noodle products, mainly for quality improvement and food preservation.
Zinc: The Metal of Life
Authors: Kaur Kuljeet , Gupta Rajiv , Saraf Shubhini A. , Saraf Shailendra K. 
Published: June 2014

AbstractThe importance of zinc was 1st reported for Aspergillus niger. It took over 75 y to realize that zinc is also an essential trace element for rats, and an additional 30 y went by before it was recognized that this was also true for humans. The adult body contains about 2 to 3 g of zinc. Zinc is found in organs, tissues, bones, fluids, and cells. It is essential for many physiological functions and plays a significant role in a number of enzyme actions in the living systems. Bioinformatics estimates report that 10% of the human proteome contains zinc‐binding sites. Based on its role in such a plethora of cellular components, zinc has diverse biological functions from enzymatic catalysis to playing a crucial role in cellular neuronal systems. Thus, based on the various published studies and reports, it is pertinent to state that zinc is one of the most important essential trace metals in human nutrition and lifestyle. Its deficiency may severely affect the homeostasis of a biological system. This review compiles the role of zinc in prophylaxis/therapeutics and provides current information about its effect on living beings.
Structure, Physicochemical Properties, Modifications, and Uses of Sorghum Starch
Authors: Zhu Fan 
Published: June 2014

AbstractSorghum has great drought resistance and can be cultivated on marginal lands with minimal input costs. Thus, the supply of sorghum starch, the major component of the seeds, can be cheap and sustainable. This review summarizes the present knowledge of extraction, composition, granular and molecular structures, physicochemical properties, modifications, and uses of sorghum starch, and provides suggestions for needed research to improve our understanding and utilization of this starch.
Molecular Methods in Food Safety Microbiology: Interpretation and Implications of Nucleic Acid Detection
Authors: Ceuppens Siele , Li Dan , Uyttendaele Mieke , Renault Pierre , Ross Paul , Ranst Marc Van , Cocolin Luca , Donaghy John 
Published: June 2014

AbstractBecause of increasing demand for rapid results, molecular techniques are now applied for the detection of microorganisms in foodstuffs. However, interpretation problems can arise for the results generated by molecular methods in relation to the associated public health risk. Discrepancies between results obtained by molecular and conventional culture methods stem from the difference in target, namely nucleic acids instead of actively growing microorganisms. Nucleic acids constitute 5% to 15% of the dry weight of all living cells and are relatively stable, even after cell death, so they may be present in a food matrix after the foodborne microorganisms have been inactivated. Therefore, interpretation of the public health significance of positive results generated by nucleic acid detection methods warrants some additional consideration. This review discusses the stability of nucleic acids in general and highlights the persistence of microbial nucleic acids after diverse food‐processing techniques based on data from the scientific literature. Considerable amounts of DNA and RNA (intact or fragmented) persist after inactivation of bacteria and viruses by most of the commonly applied treatments in the food industry. An overview of the existing adaptations for molecular assays to cope with these problems is provided, including large fragment amplification, flotation, (enzymatic) pretreatment, and various binding assays. Finally, the negligible risks of ingesting free microbial nucleic acids are discussed and this review ends with the future perspectives of molecular methods such as next‐generation sequencing in diagnostic and source attribution food microbiology.
Methods to Evaluate Uptake of Engineered Nanomaterials by the Alimentary Tract
Authors: Alger Heather , Momcilovic Dragan , Carlander David , Duncan Timothy V. 
Published: June 2014

AbstractThis article is one of a series of 4 that report on a task of the NanoRelease Food Additive project of the International Life Science Institute Center for Risk Science Innovation and Application to identify, evaluate, and develop methods that are needed to confidently detect, characterize, and quantify intentionally produced engineered nanomaterials (ENMs) released from food along the alimentary tract. This particular article focuses on the problem of detecting and characterizing ENMs in the various compartments of the alimentary tract after they have been ingested from dietary sources. An in depth analysis of the literature related to oral toxicity of ENMs is presented, paying particular attention to analytical methodology and sample preparation. The review includes a discussion of model systems that can be used to study oral uptake of ENMs in the absence of human toxicological data or other live‐animal studies. The strengths and weaknesses of various analytical and sample preparation techniques are discussed. The article concludes with a summary of findings and a discussion of potential knowledge gaps and targets for method development in this area.
Measurement Methods for the Oral Uptake of Engineered Nanomaterials from Human Dietary Sources: Summary and Outlook
Authors: Szakal Christopher , Tsytsikova Lyubov , Carlander David , Duncan Timothy V. 
Published: June 2014

AbstractThis article is one of a series of 4 that report on a task of the NanoRelease Food Additive (NRFA) project of the International. Life Science Institute Center for Risk Science Innovation and Application. The project aims are to identify, evaluate, and develop methods that are needed to confidently detect, characterize, and quantify intentionally produced engineered nanomaterials (ENMs) released from food along the alimentary tract. This particular article offers an overview of the NRFA project, describing the project scope and goals, as well as the strategy by which the task group sought to achieve these goals. A condensed description of the general challenge of detecting ENMs in foods and a brief review of available and emerging methods for ENM detection is provided here, paying particular attention to the kind of information that might be desired from an analysis and the strengths and weaknesses of the various approaches that might be used to attain this information. The article concludes with an executive summary of the task group's broad findings related to the 3 topic areas, which are covered in more detail in 3 subsequent articles in this series. The end result is a thorough evaluation of the state of ENM measurement science specifically as it applies to oral uptake of ENMs from human dietary sources.
In Vitro Models for Studying Secondary Plant Metabolite Digestion and Bioaccessibility
Authors: Alminger M. , Aura A.‐M. , Bohn T. , Dufour C. , El S.N. , Gomes A. , Karakaya S. , Martínez‐Cuesta M.C. , McDougall G.J. , Requena T. , Santos C.N. 
Published: June 2014

AbstractThere is an increased interest in secondary plant metabolites, such as polyphenols and carotenoids, due to their proposed health benefits. Much attention has focused on their bioavailability, a prerequisite for further physiological functions. As human studies are time consuming, costly, and restricted by ethical concerns, in vitro models for investigating the effects of digestion on these compounds have been developed and employed to predict their release from the food matrix, bioaccessibility, and assess changes in their profiles prior to absorption. Most typically, models simulate digestion in the oral cavity, the stomach, the small intestine, and, occasionally, the large intestine. A plethora of models have been reported, the choice mostly driven by the type of phytochemical studied, whether the purpose is screening or studying under close physiological conditions, and the availability of the model systems. Unfortunately, the diversity of model conditions has hampered the ability to compare results across different studies. For example, there is substantial variability in the time of digestion, concentrations of salts, enzymes, and bile acids used, pH, the inclusion of various digestion stages; and whether chosen conditions are static (with fixed concentrations of enzymes, bile salts, digesta, and so on) or dynamic (varying concentrations of these constituents). This review presents an overview of models that have been employed to study the digestion of both lipophilic and hydrophilic phytochemicals, comparing digestive conditions in vitro and in vivo and, finally, suggests a set of parameters for static models that resemble physiological conditions.
Ice Cream as a Vehicle for Incorporating Health‐Promoting Ingredients: Conceptualization and Overview of Quality and Storage Stability
Authors: Soukoulis Christos , Fisk Ian D. , Bohn Torsten 
Published: June 2014

AbstractIce cream is a product with peculiar textural and organoleptic features and is highly appreciated by a very broad spectrum of consumers. Ice cream's structure and colloidal design, together with its low‐temperature storage, renders it a very promising carrier for the stabilization and in vivo delivery of bioactive compounds and beneficial microorganisms. To date, many applications related to the design and development of functional ice cream have been documented, including products containing probiotics, prebiotics, synbiotics, dietary fibers, natural antioxidants such as polyphenols, essential and polyunsaturated fatty acids, and low glycemic index blends and blends fortified with mineral or trace elements. In this review, promising strategies for the incorporation of innovative functional additives to ice cream through the use of techniques such as microencapsulation, nanoemulsions, and oleogels are discussed, and current insights into the implications of matrix, processing, and digestion on bioactive compounds in frozen dairy desserts are comprehensively reviewed, thereby providing a holistic overview of the current and emerging trends in this functional food sector.
Applications and Perceptions of Date Labeling of Food
Authors: Newsome Rosetta , Balestrini Chris G. , Baum Mitzi D. , Corby Joseph , Fisher William , Goodburn Kaarin , Labuza Theodore P. , Prince Gale , Thesmar Hilary S. , Yiannas Frank 
Published: June 2014

AbstractOpen dating of food products has been practiced for decades, and has been key to achieving stock rotation at retail and providing information to consumers. The open date provides a simple communication tool, which may be based on product quality and/or food safety as determined by the manufacturer or retailer. Date marking is generally open but it can be closed (code intended for managing product at retail, and for recall and traceability), and the terminology and applications vary widely around the world. The variation in date labeling terms and uses contributes to substantial misunderstanding by industry and consumers and leads to significant unnecessary food loss and waste, misapplication of limited resources, unnecessary financial burden for the consumer and the food industry, and may also lead to potential food safety risk in regards to perishable foods. A “use by” or similar date cannot be relied on to indicate or guarantee food safety because absolute temperature control of food products throughout the food supply chain cannot be assured. This paper provides an introduction to the issue of food product date labeling and addresses its history in the United States, different terms used and various practices, U.S. and international frameworks, quality compared with safety, adverse impacts of misconceptions about date labeling, and advantages of technological innovations. Collaboration to develop a simple workable solution to address the challenges faced by stakeholders would have tremendous benefit. Conclusions include a call to action to move toward uniformity in date labeling, thereby decreasing confusion among stakeholders and reducing food waste.