bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic analysis of the class Phycisphaerae reveals a versatile group of complex carbon-degrading bacteria
Authors: Lenferink Wouter B. , van Alen Theo A. , Jetten Mike S. M. , Op den Camp Huub J. M. , van Kessel Maartje A. H. J. , Lücker Sebastian 
Published: July 2024

AbstractBacteria of the phylum Planctomycetota have received much attention over the years due to their unique cell biology and potential for biotechnological application. Within the phylum, bacteria of the class Phycisphaerae have been found in a multitude of environmental datasets. However, only a few species have been brought into culture so far and even enrichments are scarce. Therefore, very little is known about their lifestyle, which has hindered efforts to estimate their environmental relevance. Here, we analysed all medium- and high-quality Phycisphaerae genomes represented in the genome taxonomy database to learn more about their physiology. We combined automatic and manual annotation efforts to provide a bird’s eye view of their diverse energy metabolisms. Contrasting previous reports, we did not find indications for the presence of genes for anaerobic ammonium oxidation in any Phycisphaerae genome. Instead, we found that many members of this class are adapted to a facultative anaerobic or strictly fermentative lifestyle and may be specialized in the breakdown of carbon compounds produced by other organisms. Based on these findings, we provide a practical overview of organic carbon substrates predicted to be utilized by Phycisphaerae families.
Artificial intelligence-derived left ventricular strain in echocardiography in patients treated with chemotherapy
Authors: Kuwahara Asuka , Iwasaki Yoichi , Kobayashi Masatake , Takagi Ryu , Yamada Satoshi , Kubo Takashi , Satomi Kazuhiro , Tanaka Nobuhiro 
Published: July 2024

AbstractGlobal longitudinal strain (GLS) is an echocardiographic measure to detect chemotherapy-related cardiovascular dysfunction. However, its limited availability and the needed expertise may restrict its generalization. Artificial intelligence (AI)-based GLS might overcome these challenges. Our aims are to explore the agreements between AI-based GLS and conventional GLS, and to assess whether the agreements were influenced by expertise levels, cardiac remodeling and cardiovascular diseases/risks. Echocardiographic images in the apical four-chamber view of left ventricle were retrospectively analyzed based on AI-based GLS in patients treated with chemotherapy, and correlation between AI-based GLS (Caas Qardia, Pie Medical Imaging) and conventional GLS (Vivid E9/VividE95, GE Healthcare) were assessed. The agreement between unexperienced physicians (“GLS beginner”) and experienced echocardiographer were also assessed. Among 94 patients (mean age 69 ± 12 years, 73% female), mean left ventricular ejection fraction was 64 ± 6%, 14% of patients had left ventricular hypertrophy, and 21% had left atrial enlargement. Mean GLS was − 15.9 ± 3.4% and − 19.0 ± 3.7% for the AI and conventional method, respectively. There was a moderate correlation between these methods (rho = 0.74; p < 0.01), and bias was − 3.1% (95% limits of agreement: -8.1 to 2.0). The reproducibility between GLS beginner and an experienced echocardiographer was numerically better in the AI method than the conventional method (inter-observer agreement = 0.82 vs. 0.68). The agreements were consistent across abnormal cardiac structure and function categories (p-for-interaction > 0.10). In patients treated with chemotherapy. AI-based GLS was moderately correlated with conventional GLS and provided a numerically better reproducibility compared with conventional GLS, regardless of different levels of expertise.
Machine Learning Predicts Unplanned Care Escalations for Post-Anesthesia Care Unit Patients during the Perioperative Period: A Single-Center Retrospective Study
Authors: Barker Andrew B. , Melvin Ryan L. , Godwin Ryan C. , Benz David , Wagener Brant M. 
Published: July 2024

Abstract

Background: Despite low mortality for elective procedures in the United States and developed countries, some patients have unexpected care escalations (UCE) following post-anesthesia care unit (PACU) discharge. Studies indicate patient risk factors for UCE, but determining which factors are most important is unclear. Machine learning (ML) can predict clinical events. We hypothesized that ML could predict patient UCE after PACU discharge in surgical patients and identify specific risk factors. Methods: We conducted a single center, retrospective analysis of all patients undergoing non-cardiac surgery (elective and emergent). We collected data from pre-operative visits, intra-operative records, PACU admissions, and the rate of UCE. We trained a ML model with this data and tested the model on an independent data set to determine its efficacy. Finally, we evaluated the individual patient and clinical factors most likely to predict UCE risk. Results: Our study revealed that ML could predict UCE risk which was approximately 5% in both the training and testing groups. We were able to identify patient risk factors such as patient vital signs, emergent procedure, ASA Status, and non-surgical anesthesia time as significant variable. We plotted Shapley values for significant variables for each patient to help determine which of these variables had the greatest effect on UCE risk. Of note, the UCE risk factors identified frequently by ML were in alignment with anesthesiologist clinical practice and the current literature. Conclusions: We used ML to analyze data from a single-center, retrospective cohort of non-cardiac surgical patients, some of whom had an UCE. ML assigned risk prediction for patients to have UCE and determined perioperative factors associated with increased risk. We advocate to use ML to augment anesthesiologist clinical decision-making, help decide proper disposition from the PACU, and ensure the safest possible care of our patients.
Gelation and Localization in Multicomponent Coagulation with Multiplicative Kernel Through Branching Processes
Authors: Hoogendijk Jochem , Kryven Ivan , Schenone Camillo 
Published: July 2024

AbstractThe multicomponent coagulation equation is a generalization of the Smoluchowski coagulation equation, where the size of a particle is described by a vector. Similar to the original Smoluchowski equation, the multicomponent coagulation equation exhibits gelation behavior when supplied with a multiplicative kernel. Additionally, a new type of behaviour called localization is observed due to the multivariate nature of the particle size distribution. Here we extend the branching process representation technique, which we introduced to study differential equations in our previous work, and apply it to find a concise probabilistic solution of the multicomponent coagulation equation supplied with monodisperse initial conditions. We also provide short proofs for the gelation time and characterisation the localization phenomenon.
Exploring the relationship between caffeine metabolism-related CYP1A2 rs762551 polymorphism and team sport athlete status and training adaptations
Authors: Kazan Hasan H. , Bulgay Celal , Zorba Ercan , Dalip Metin , Ceylan Halil İ. , Semenova Ekaterina A. , Larin Andrey K. , Kulemin Nikolay A. , Generozov Edward V. , Ahmetov Ildus I. , Cerit Mesut 
Published: July 2024

Abstract

Background
This study aimed to achieve a dual objective: to compare the frequencies of CYP1A2 rs762551 genotypes between team sport athletes and a control group, and to determine the association between the rs762551 polymorphism and changes in physical performance after a six-week training program among elite basketball players.

Methods
The study encompassed an analysis of 504 individuals, comprising 320 athletes and 184 controls. For the Turkish cohort, DNA was isolated using the buccal swab method, and genotyping was conducted using the KASP technique. Performance assessments included the Yo-Yo IR2 and 30 m sprint tests. For Russian participants, DNA samples were extracted from peripheral blood, a commercial kit was used for DNA extraction, and genotyping of the rs762551 polymorphism was conducted using DNA microarray.

Result
Notably, a statistically significant linear decline in the prevalence of the CC genotype was observed with ascending levels of athletic achievement within team sports (sub-elite: 18.0%, elite: 8.2%, highly elite: 0%; p = 0.001). Additionally, the CA genotype was the most prevalent genotype in the highly elite group compared to controls (80.0% vs. 45.1%, p = 0.048). Furthermore, statistically significant improvements in Yo-Yo IR2 performance were noted exclusively among basketball players harboring the CA genotype (p = 0.048).

Conclusions
The study’s findings indicate that the rs762551 CC genotype is a disadvantage in elite team sports, whereas the CA genotype provides an advantage in basketball performance.
Non-invasive prehabilitation to foster widespread fMRI cortical reorganization before brain tumor surgery: lessons from a case series
Authors: Boccuni Leonardo , Roca-Ventura Alba , Buloz-Osorio Edgar , Leno-Colorado David , Delgado-Gallén Selma , Cabello-Toscano María , Perellón-Alfonso Ruben , Villalba-Martínez Gloria , Martínez-Ricarte Francisco , Martín-Fernández Jesús , Buxeda-Rodriguez Mònica , Conesa-Bertrán Gerardo , Illueca-Moreno Mireia , Lladó-Carbó Estela , Perla y Perla Cristóbal , Garrido César , Pariente José Carlos , Laredo Carlos , Muñoz-Moreno Emma , Bargalló Núria , Trompetto Carlo , Marinelli Lucio , Bartrés-Faz David , Abellaneda-Pérez Kilian , Pascual-Leone Alvaro , Tormos-Muñoz Josep María 
Published: July 2024

Abstract
Purpose
The objective of this prospective, single-centre case series was to investigate feasibility, clinical outcomes, and neural correlates of non-invasive Neuromodulation-Induced Cortical Prehabilitation (NICP) before brain tumor surgery. Previous studies have shown that gross total resection is paramount to increase life expectancy but is counterbalanced by the need of preserving critical functional areas. NICP aims at expanding functional margins for extensive tumor resection without functional sequelae. Invasive NICP (intracranial neuromodulation) was effective but characterized by elevated costs and high rate of adverse events. Non-invasive NICP (transcranial neuromodulation) may represent a more feasible alternative. Nonetheless, up to this point, non-invasive NICP has been examined in only two case reports, yielding inconclusive findings.

Methods
Treatment sessions consisted of non-invasive neuromodulation, to transiently deactivate critical areas adjacent to the lesion, coupled with intensive functional training, to activate alternative nodes within the same functional network. Patients were evaluated pre-NICP, post-NICP, and at follow-up post-surgery.

Results
Ten patients performed the intervention. Feasibility criteria were met (retention, adherence, safety, and patient’s satisfaction). Clinical outcomes showed overall stability and improvements in motor and executive function from pre- to post-NICP, and at follow-up. Relevant plasticity changes (increase in the distance between tumor and critical area) were observed when the neuromodulation target was guided by functional neuroimaging data.

Conclusion
This is the first case series demonstrating feasibility of non-invasive NICP. Neural correlates indicate that neuroimaging-guided target selection may represent a valid strategy to leverage neuroplastic changes before neurosurgery. Further investigations are needed to confirm such preliminary findings.
How budgets change: punctuations, trends, and super-trends
Authors: Segal Ehud , Baumgartner Frank R. 
Published: July 2024

AbstractPunctuated equilibrium theory (PET) describes policy change as occurring mostly through incremental movements with infrequent periods of dramatic change. An impressive body of empirical literature relating to budgeting supports this view, but virtually all empirical tests have focused on examining distributions of annual changes, thus nullifying chronology. In this article, we focus on the time element. Using the same databases as previously used in canonical PET studies, we explore multi-year trends, not only annual observations. For our analyses, we identify directional series of changes (while allowing for one-year changes in direction if these are immediately offset in the following year) on a U.S. budget distribution dataset covering the period of 1947 through 2014, with 60 categories of spending consistently defined over time and adjusted for inflation. We then assess the robustness of the PET findings when incorporating a longer time units of trending series of annual changes into the analysis. We find that almost 65% of changes occur in series of 4 years or more. Nonetheless, the signature PET literature pattern of high kurtosis is equally present in these series as well as in shorter series. Moreover, within growing and trending series, we find that 21% of these series generate 80% of positive budget change. Within these series, we identify a small group of “super-trends” that account for a large share of the overall change. We conclude that expanding methodologies for the study of budgetary change to incorporate longer-term dynamics helps to better understand policy change, but such findings remain consistent with the PET perspective.
Correlates of stocking naloxone: a cross-sectional survey of community pharmacists
Authors: Laing Rose , Xia Ting , Grist Elizabeth , Dostal Jana , Nielsen Suzanne , Picco Louisa 
Published: July 2024

Abstract
Background
Provision of take-home naloxone (THN) and overdose education reduces opioid-related mortality. In Australia, from July 2022, all Australian community pharmacies were eligible to supply naloxone for free through the national THN Program.

Aim
This study aimed to identify naloxone stocking rates and correlates of stocking naloxone across Australian pharmacies.

Method
Data were collected from a representative sample of Australian pharmacists in Victoria, New South Wales, Queensland and Western Australia via an online survey. Data collected included pharmacy and pharmacist characteristics and services offered within the pharmacy, including needle and syringe programs, opioid agonist treatment (OAT) and stocking naloxone. Binary probit regression analysis was used to identify correlates of stocking naloxone after controlling for key covariates.

Results
Data from 530 pharmacists were analysed. In total, 321 pharmacies (60.6%) reported stocking naloxone. Chain pharmacies and pharmacies that provided OAT had a greater probability of stocking naloxone (B = 0.307, 95%CI: [0.057, 0.556], and B = 0.543, 95%CI: [0.308, 0.777] respectively). Most (61.7%) pharmacists felt comfortable discussing overdose prevention with patients who use prescription opioids, and this comfort was associated with a higher probability of stocking naloxone (B = 0.392, 95%CI: 0.128, 0.655). Comfort discussing overdose prevention with people who use illicit opioids was lower (49.4%) and was not associated with stocking naloxone.

Conclusion
There is scope to increase stocking of naloxone and comfort with overdose prevention, particularly through addressing comfort working with higher risk groups such as people who use illicit opioids.
Word recognition thresholds in novice readers: exploring when reading and listening comprehension are comparable
Authors: García J. Ricardo , Sánchez Emilio , Calvo Natalia , Cain Kate 
Published: July 2024

AbstractThis study proposes and identifies two levels of word reading at which good comprehenders can obtain a similar understanding of written and spoken narrative texts: The oral-written matching functional and efficient thresholds. These thresholds are compared with the level of word reading over which a relationship between listening comprehension and reading comprehension is evident (a basic word recognition threshold) and with the level after which better word recognition does not result in improvements in reading comprehension (an upper word recognition threshold). The three first thresholds were calculated in a sample of 344 first to third-grade Spanish students who read and listened to two narrative texts of different difficulty and who were assessed on measures of comprehension and word recognition. Listening and reading comprehension were related when participants accurately read more than 73% of pseudowords in a list (basic word recognition threshold). For participants with good listening comprehension, reading comprehension performance matched listening comprehension once a moderate reading speed of ~ 66 decontextualized words per minute was achieved (oral-written matching functional threshold) or when the rate of reading the text was ~ 140 words per minute (oral-written matching efficient threshold). The value of the oral-written matching efficient threshold was, in this study, similar to the upper word recognition threshold identified by prior research. The thresholds calculated in this study delineate a sequence of increasingly challenges to the developing reader, allowing a nuanced description of the initial stages of reading development.
A Counterfactual Analysis of Amazon’s Acquisitions Under the 2023 Merger Guidelines
Authors: Snyder Edward A. , Simmons Ian , Zaslavsky Sergei 
Published: July 2024

AbstractThe fact that Amazon was allowed to acquire hundreds of companies as it rose to become the fourth most valuable U.S. company in terms of market capitalization and a leader in three lines of business has been viewed by some as damning evidence of underenforcement by the United States antitrust authorities. In this article we ask the obvious question: If the 2023 Guidelines had been in place instead of prior guidelines, what effects would they have had on Amazon’s development? To provide an answer, we identify relevant changes in the guidelines and then select for review a subset of Amazon’s 280 acquisitions over the period 1998 to 2022. In our counterfactual, we analyze five horizontal acquisitions, four vertical acquisitions, and two sets ofserial acquisitions. We find that the 2023 Guidelines would have broadened the bases for potential challenges and thereby would have increased the likelihood that Amazon would have faced greater resistance from antitrust authorities. The lack of safe harbors, the plasticity of individual Guidelines, and the optionality to challenge mergers under alternative theories would have exposed most of Amazon’s acquisitions to challenge. The lack of meaningful guidance about which individual transactions would have been challenged suggests that going forward enforcer discretion will play a yet larger role. Regarding Amazon’s serial acquisitions of nearly one hundred technology firms, we find that the 2023 Guidelines would have provided multiple rationales for intervention. Therein lies a weakness in the Guidelines and in antitrust policy: the lack of a framework for assessing both the anticompetitive and procompetitive effects of such acquisitions in high-tech industries.
Enhancing High-Pressure Bacterial Inactivation by Modified Atmosphere Packaging: Effect of Exposure Time and Cooked Ham Formulation
Authors: Serra-Castelló Cristina , Jofré Anna , Bover-Cid Sara 
Published: July 2024

Abstract
High-pressure processing (HPP) is a non-thermal preservation technology that can be applied as a control measure to inactivate pathogens and spoilage microorganisms once RTE meat products are packaged in a convenient format. HPP efficacy highly depends on product characteristics, but the impact of the sodium-reduced formulations and the effect of packaging atmosphere are scarcely known. The aim of the present work was to assess the effect of standard and sodium-reduced formulations from two different brands (A, B) under different packaging (vacuum and modified atmosphere packaging (MAP)) on the HPP inactivation kinetics of Listeria monocytogenes and spoilage lactic acid bacteria in cooked ham. Slices of cooked ham with standard and sodium-reduced formulations were inoculated with L. monocytogenes CTC1034 and Latilactobacillus sakei CTC746 (slime producer), packaged in vacuum and MAP (CO2:N2, 20:80), and pressurized (400 MPa/0–15 min) after 1 h (vacuum, MAP) or 24 h (MAP-exposed). Parameters of HPP inactivation kinetics were estimated by fitting the Weibull model to log reduction data. Results showed that the efficacy of HPP in sodium-reduced cooked hams tended to decrease compared to standard formulations, being the difference statistically significant for L. sakei. For L. monocytogenes, a significant enhancing effect of MAP was observed when HPP was applied just after packaging (1 h, MAP) of cooked ham of brand A. In the case of L. sakei, the inactivation by HPP was only enhanced in MAP-exposed samples. Therefore, the use of HPP as a control measure must be applied through a product-oriented approach considering the type of packaging and the time period between packaging and HPP.
Effects of Diel Oxygen Cycling and Benthic Macrofauna on Sediment Oxygen Demand
Authors: Gadeken Kara J. , Dorgan Kelly M. 
Published: July 2024

AbstractThis field study examined how sediment macroinfauna change patterns of sediment oxygen demand (SOD) throughout a diel oxygen cycle. Sediments with a greater faunal presence would be expected to have greater overall SOD, and at night may alter their behavior and influence SOD depending on their response to low-oxygen stress. Dynamic faunal bioturbation or bioirrigation behavior would also result in corresponding variation in SOD values on short time scales. In situ flow-through benthic metabolism chambers were used to measure SOD at a high temporal resolution in discrete sediment patches. Sediments with more macroinfauna had greater average SOD over the diel cycle, consistent with previous studies. Where more macroinfauna were present, they drove greater SOD during nightly low oxygen, presumably by enhancing their burrowing and irrigation activities. SOD was also more variable on a sub-diel timescale in sediments with more macroinfauna. Sediment oxygen demand is dynamic and highly sensitive both temporally, on very short timescales, and spatially, in terms of resident fauna, and their interaction produces heretofore unaccounted complexity in patterns of SOD particularly in shallow coastal systems. Extrapolations of temporally and spatially limited SOD measurements to a system-wide scale that do not account for the short-term and spatially variable effects of fauna may produce imprecise and misleading estimates of this critical ecosystem function.