bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of methods for cell harvesting and the biological properties at successive passages of canine bone marrow stromal cells
Authors: Nishida Hidetaka , Shoji Yuki , Nakamura Michi , Hatoya Shingo , Sugiura Kikuya , Yamate Jyoji , Kuwamura Mitsuru , Kotani Takao , Nakayama Masanari , Suzuki Yoshihisa , Ide Chizuka , Inaba Toshio 
Published: October 2012

Abstract
Objective—To compare methods for harvesting canine bone marrow stromal cells (BMSCs) and determine the biological properties of canine BMSCs at successive passages in vitro.
Sample—BMSCs collected from the femurs of 9 Beagles.
Procedures—A fibroblast assay was performed to compare 2 methods for harvesting BMSCs: the aspiration and perfusion method. Flow cytometric analysis was performed to evaluate the cell surface markers. Changes in proliferative activity were analyzed by examining radioactivity of hydrogen 3-thymidine. Cell senescence was studied via senescence-associated β-galactosidase staining, and differentiation properties (osteogenesis and adipogenesis) were estimated in association with passage.
Results—The aspiration method yielded significantly more fibroblasts than the perfusion method. The cells harvested by both methods gave positive results for CD44 and CD90 and negative results for CD34 and CD45. After induction, the cells had osteogenic and adipogenic phenotypes. The biological properties of BMSCs harvested by the aspiration method were estimated in association with passage. With increasing number of passages, the proliferative activity was reduced and the proportion of cells with senescence-associated β-galactosidase staining was increased. The capacity of differentiation was reduced at passage 3.
Conclusions and Clinical Relevance—The aspiration method was superior for collection of BMSCs. In early passages, canine BMSCs had the proliferative activity and potential of osteogenic and adipogenic differentiation, but this decreased with increased number of passages. Consideration of passage will be important to the success of any strategy that seeks to regenerate tissue though the use of BMSCs.
Comparison of pharmacokinetic variables for creatinine and iohexol in dogs with various degrees of renal function
Authors: Collignon Cécile M. , Heiene Reidun , Queau Yann , Reynolds Brice S. , Craig Amanda J. , Concordet Didier , Harran Nathaniel X. , Risøen Unni , Balouka David , Faucher Mathieu R. , Eliassen Knut A. , Biourge Vincent , Lefebvre Hervé P. 
Published: October 2012

Abstract
Objective—To compare pharmacokinetics and clearances of creatinine and iohexol as estimates of glomerular filtration rate (GFR) in dogs with various degrees of renal function.
Animals—50 Great Anglo-Francais Tricolor Hounds with various degrees of renal function.
Procedures—Boluses of iohexol (40 mg/kg) and creatinine (647 mg/kg) were injected IV. Blood samples were collected before administration and 5 and 10 minutes and 1, 2, 4, 6, and 8 hours after administration. Plasma creatinine and iohexol concentrations were assayed via an enzymatic method and high-performance liquid chromatography, respectively. A noncompartmental approach was used for pharmacokinetic analysis. Pharmacokinetic variables were compared via a Bland-Altman plot and an ANOVA.
Results—Compared with results for creatinine, iohexol had a significantly higher mean ± SD plasma clearance (3.4 ± 0.8 mL/min/kg vs 3.0 ± 0.7 mL/min/kg) and a significantly lower mean volume of distribution at steady state (250 ± 37 mL/kg vs 539 ± 73 mL/kg), mean residence time (80 ± 31 minutes vs 195 ± 73 minutes), and mean elimination half-life (74 ± 20 minutes vs 173 ± 53 minutes). Despite discrepancies between clearances, especially for high values, the difference was < 0.6 mL/min/kg for 34 (68%) dogs. Three dogs with a low GFR (< 2 mL/min/kg) were classified similarly by both methods.
Conclusions and Clinical Relevance—Plasma iohexol and creatinine clearances can be used interchangeably for screening patients suspected of having chronic kidney disease (ie, low GFR), but large differences may exist for dogs with a GFR within or above the reference range.
Multipliers and Wiener-Hopf operators on weighted L p spaces
Authors: Petkova Violeta 
Published: October 2012

AbstractWe study multipliers M (bounded operators commuting with translations) on weighted spaces L ω p (ℝ), and establish the existence of a symbol µM for M, and some spectral results for translations S t and multipliers. We also study operators T on the weighted space L ω p (ℝ+) commuting either with the right translations S t , t ∈ ℝ+, or left translations P +S −t , t ∈ ℝ+, and establish the existence of a symbol µ of T. We characterize completely the spectrum σ(S t ) of the operator S t proving that $\sigma (S_t ) = \{ z \in \mathbb{C}:|z| \leqslant e^{t\alpha _0 } \} ,$ where α 0 is the growth bound of (S t )t≥0. A similar result is obtained for the spectrum of (P +S −t ), t ≥ 0. Moreover, for an operator T commuting with S t , t ≥ 0, we establish the inclusion
The phenomenon of super energy flows in the anisotropic left-handed material
Authors: Xu Bai , Yu Guan , Xia Li , Wang Zhi 
Published: October 2012

AbstractThe super energy flows generated and transmitted have been investigated in a parallel-plate waveguide, which is filled with air and the anisotropic left-handed materials. Theoretical analysis and numerical simulations show that the propagation modes of the anisotropic super waveguide are consistent with those of the isotropic waveguides [1–3]. They also show that the loss of electromagnetic parameters size of waveguide will influence the amplitude of time-average power flows.
Path integral treatment of a noncentral electric potential
Authors: Ghoumaid Ali , Benamira Farid , Guechi Larbi , Khiat Zohra 
Published: October 2012

AbstractWe present a rigorous path integral treatment of a dynamical system in the axially symmetric potential $V(r,\theta ) = V(r) + \tfrac{1} {{r^2 }}V(\theta ) $ . It is shown that the Green’s function can be calculated in spherical coordinate system for $V(\theta ) = \frac{{\hbar ^2 }} {{2\mu }}\frac{{\gamma + \beta \sin ^2 \theta + \alpha \sin ^4 \theta }} {{\sin ^2 \theta \cos ^2 \theta }} $ . As an illustration, we have chosen the example of a spherical harmonic oscillator and also the Coulomb potential for the radial dependence of this noncentral potential. The ring-shaped oscillator and the Hartmann ring-shaped potential are considered as particular cases. When α = β = γ = 0, the discrete energy spectrum, the normalized wave function of the spherical oscillator and the Coulomb potential of a hydrogen-like ion, for a state of orbital quantum number l ≥ 0, are recovered.
Functionalization of SBA-15 materials for the adsorption of phenols from aqueous solution
Authors: Toufaily Joumana , Koubaissy Bachar , Kafrouny Lina , Hamad Hussein , Magnoux Patrick , Ghannam Leila , Karout Ali , Hazimeh Hassan , Nemra Ghinwa , Hamieh Moustafa , Ajouz Nawal , Hamieh Tayssir 
Published: October 2012

Abstract
Water pollution by toxic organic compounds is a problem and demand for efficient adsorbents for the removal of toxic compounds is increasing. In the present work, we studied the functionalization of SBA-15 materials via the co-condensation between an alkoxysilane and an organoalkoxysilane in the presence of P123 as structuring agent. Several types of ligands were used: 3-mercaptopropylmethyldimethoxysilane, N-[3-(trimethoxysilyl) propyl] aniline; aminopropyltrimethoxysilane; [aminoethylamino]-propyltrimethoxysilanes and [(2-aminoethylamino) ethylamino] propyltrimethoxysilanes. These materials exhibit BET surface area of 275–776 m2/g and total pore volume of 0.29–1 cm3/g, depending on the ligand types and contents. Elementary analysis results confirm the incorporation of both thiol and amine group in the materials. Batch adsorption studies shows that the adsorption capacity of phenol drifts on the amine and thiol functionalized SBA-15 is greater than that on pure SBA-15. A linear relationship was observed between the adsorption capacity and N/SiO2 ratio. It was shown that the presence of amine promotes interactions with water molecules, on the other hand, these results can also be explained by the basic behavior of N-functionalized materials.
Box behnken design based optimization of solar induced photo catalytic decolourization of textile dye effluent
Authors: Senthilkumar Shanmugam , Perumalsamy Muthiah , Prabhu Harinarayan , AhmedBasha Chiya , Swaminathan G. 
Published: October 2012

Abstract
Box-Behnken design was employed for the decolourization of synthetic dye bath effluent using solar induced photo catalytic degradation with mixed semi conductor catalysts. Four independent variables namely concentration of dye effluent, catalyst loading, pH and irradiation time was chosen as process variables. The optimum concentrations of dye effluent, catalyst dosage, pH, and irradiation time were found to be 60 mg L−1, 200 mg L−1, 7 and 100 min, respectively, for maximum decolourization of dye effluent (91.24%). Predicted values were found to be in good agreement with experimental values and as a result reflected the precision and the applicability of Response Surface Methodology (RSM) (R2=0.9785 and Adj R2= 0.9569).
Do Patients Achieve Normal Gait Patterns 3 Years After Total Knee Arthroplasty?
Authors: Yoshida Yuri , Zeni Joseph , Snyder-Mackler Lynn 
Published: October 2012

Study Design Longitudinal cross-sectional study. Background In the early stages after total knee arthroplasty (TKA), quadriceps strength of the operated limb decreases and is substantially less than that of the nonoperated limb. This asymmetry in strength is related to asymmetrical movement patterns that increase reliance on the nonoperated limb. Over time, quadriceps strength in the operated limb increases but remains less than that in age-matched controls without knee pathology, whereas the quadriceps strength in the nonoperated limb gradually decreases. The purpose of this study was to investigate the changes in quadriceps strength and function of both limbs up to 3 years after TKA and to evaluate change in interlimb kinematic and kinetic parameters over time compared to that in age-matched individuals without knee pathology. Methods Fourteen individuals after TKA and 14 healthy individuals matched for age, weight, height, and sex participated in the study. Outcome measures included kinematics, kinetics, quadriceps strength, and functional performance. Results In participants who underwent TKA, quadriceps strength was significantly different between limbs at 3 months and 1 year after TKA, but not at 3 years after TKA. In this group, there was also a significant improvement in self-reported function between 3 months and 1 year after TKA, but a significant decrease between years 1 and 3 for the physical component summary score of the Medical Outcomes Study 36-Item Short-Form Health Survey. In the TKA group, there were few interlimb differences in joint kinematics and kinetics 3 years after TKA, which may be attributed to a combination of worsening in the nonoperated limb, as well as improvement in the operated limb. Differences between participants without knee pathology and those 3 years after TKA still existed for kinematic, kinetic, and spatiotemporal variables. Conclusion As interlimb differences in quadriceps strength decrease after TKA, there are concomitant symmetrical improvements in temporospatial and kinetic gait parameters. The symmetry 3 years after TKA in quadriceps strength is primarily the result of progressive weakness in the nonoperated limb. J Orthop Sports Phys Ther 2012;42(12):1039–1049, Epub 22 October 2012. doi:10.2519/jospt.2012.3763
Habitat use, bed‐site selection and mortality rate in neonate fallow deerDama dama
Authors: Kjellander Petter , Svartholm Ida , Bergvall Ulrika A. , Jarnemo Anders 
Published: October 2012

An understanding of mortality patterns, and especially the variation in juvenile mortality, is an important component in vertebrate population dynamics. Our study investigates, for the first time, neonate mortality and two levels of spatial behaviour, in a free‐ranging fallow deerDama damapopulation in southwestern Sweden. In the summers of 2008 and 2009, 36 fawns were marked with radio‐collars. Neonate mortality calculated by the Kaplan‐Meier method was 23.6%. Mortality caused by predation was low, since only one of eight non‐surviving fawns died from predation, probably by red foxVulpes vulpes. The spatial behaviour of the neonates was examined by habitat selection at home‐range level, which in fact is a selection made by the mother, and at bed‐site level within that habitat. Compositional analysis revealed a significant preference for arable land, pasture and coniferous forest between 5‐15 m high, compared to young forest. Selected bed sites showed significantly lower visibility and higher amount of canopy cover than random sites. Surprisingly, we did not find any relationship between canopy cover and visibility in selected bed sites while it showed a significant and negative relationship at random bed sites. We interpret this finding as while high canopy cover and low visibility covary at the habitat level, fawns seem to select these two bed‐site variables independently, perhaps for thermoregulatory reasons. Since there are few predators in our study area and predation pressure is low, this behaviour is not connected to actual survival rates in this area, but would rather be in support of the hypothesis of ‘pleiotropy’ as thermoregulatory reasons for bed‐site selection in neonate fawns might be the most important contemporary selection force in the absence of large predators.
Intra‐seasonal variation in wolf Canis lupus kill rates
Authors: Vucetich John A. , Huntzinger Brett A. , Peterson Rolf O. , Vucetich Leah M. , Hammill James H. , Beyer Dean E. 
Published: October 2012

Knowing kill rate is essential for knowing the basic nature of predation. We compared estimates of kill rate for previously observed wolf‐prey systems with new observations from wolves Canis lupus which preyed on white‐tailed deer Odocoileus virginianus in Michigan, USA. For the five packs that we studied during 2001‐2004, the mean kill rate was 0.68 kill/pack/day (∼7.7 kg/wolf/day). However, kill rates varied considerably. In particular, the coefficient of variation associated with the means was 0.55 for kills/pack/day and 0.68 for kg/wolf/day. Our analysis of previously observed kill rates also revealed a negative correlation between the duration of observation and the estimated kill rate. This correlation is the basis for showing how most published estimates of kill rate for wolves during winter tend to overestimate, by 50%, the season‐long average kill rate during winter. The negative association between duration of observation and estimated kill rate occurs, in part, because wolves are unable to maintain very high kill rates for a long time. We also document how estimates of kill rate based on ground tracking tended to be 3.3 times greater than aerial‐based estimates (2.4 vs 7.9 kg/wolf/day). Ground tracking is better able to detect multiple carcasses at one site, and better able to detect carcasses when wolves bed far from their kills. These previously undocumented biases are surprising given that wolves are so extensively studied.
Israel, 1948 and Memoricide: The 1948 Al-‘Araqib/Negev Massacre and its Legacy
Authors: Rego Nasser 
Published: October 2012

In September 1948 fourteen young and middle-aged Palestinian Bedouin men from the Naqab (Negev), most tending to fields and livestock, were rounded up on a Zionist army vehicle and driven to the abandoned home of refugee ‘Odeh al-Qawasmeh in al-‘Araqib and summarily executed. More recently, on 27 July 2010, the entire village of al-‘Araqib was demolished by Israeli security forces in what locals have termed ‘the new Nakba’. This article attempts to construct an ‘authentic history' around al-‘Araqib by linking the ‘cleansing’ operations in 1948 and those in 2010, by weaving together native accounts and silences in the historical record. It challenges contemporary historiography's tendency to discount native testimony and to banalise violence in this case of ‘ongoing colonialism’. By drawing in the example of the political persecution of a local activist attempting to construct such an authentic history, the article draws attention to the various currents that aim at effecting a memoricide of al-‘Araqib.
Le discours et la matérialité de ses artefacts
Authors: Krippendorff Klaus , Bahroun Allan , Candel Étienne 
Published: October 2012

Klaus Krippendorff, représentant majeur du courant cybernétique et du constructivisme social, propose dans cet article une théorie du discours susceptible de prendre en compte la matérialité des artefacts, de la dimension signifiante des textes et du mouvement de réinterprétation dont ils font l’objet. En mettant en évidence la façon dont la linguistique, l’analyse conversationnelle, l’analyse de discours et l’analyse de contenu négligent une part essentielle des discours sociaux, l’auteur propose de repenser le discours comme une réalité qui s’auto-contraint, à partir de cinq éléments constitutifs : les artefacts qu’il produit, les communautés de praticiens qui le font vivre, les pratiques récurrentes qu’il institue, les frontières qu’il trace et son pouvoir de justification. Cette analyse débouche sur une reformulation de la relation entre discours et réalité qui se détache d’une conception représentationnelle pour se fonder sur une pragmatique de l’ affordance , qui permet de comprendre comment les discours et leurs praticiens s’adaptent à différents environnements et entrent en contact avec des discours autres.
Continuer le Festival d’Avignon
Authors: Malinas Damien , Pourquier-Jacquin Stéphanie , Pamart Émilie , Roth Raphaël 
Published: October 2012

Lieu de l’incarnation du mythe du théâtre populaire, le festival d’Avignon, créé en 1947 par Jean Vilar, suscite encore toutes les questions relatives aux publics. S’il n’y a pas à proprement parler d’auteur(s) du festival d’Avignon, son dispositif et sa forme se pérennisent, évoluent et continuent de rencontrer l’adhésion du public. De fait, comprendre la « fonction-auteur » du festival d’Avignon nécessite d’interroger cette croyance et les différents niveaux d’adhésion qu’il implique. C’est ce que propose de faire cette contribution dans un premier temps avant de décrire dans ses évolutions le fonctionnement du mythe « Avignon » puis d’analyser comment Avignon a assigné une place singulière à l’expérience festivalière et comment celle-ci définit ce que signifie « faire le festival ».
Experience of quality management system in a clinical laboratory in Nigeria
Authors: Audu Rosemary A. , Sylvester-Ikondu Ugochukwu , Onwuamah Chika K. , Salu Olumuyiwa B. , Ige Fehintola A. , Meshack Emily , Aniedobe Maureen , Amoo Olufemi S. , Okwuraiwe Azuka P. , Okhiku Florence , Okoli Chika L. , Fasela Emmanuel O. , Odewale Ebenezer O. , Aleshinloye Roseline O. , Olatunji Micheal , Idigbe Emmanuel O. 
Published: October 2012

Issues: Quality-management systems (QMS) are uncommon in clinical laboratories in Nigeria, and until recently, none of the nation’s 5 349 clinical laboratories have been able to attain the certifications necessary to begin the process of attaining international accreditation. Nigeria’s Human Virology Laboratory (HVL), however, began implementation of a QMS in 2006, and in 2008 it was determined that the laboratory conformed to the requirements of ISO 9001:2000 (now 2008), making it the first diagnostic laboratory to be certified in Nigeria. The HVL has now applied for the World Health Organization (WHO) accreditation preparedness scheme. The experience of the QMS implementation process and the lessons learned therein are shared here.Description: In 2005, two personnel from the HVL spent time studying quality systems in a certified clinical laboratory in Dakar, Senegal. Following this peer-to-peer technical assistance, several training sessions were undertaken by HVL staff, a baseline assessment was conducted, and processes were established. The HVL has monitored its quality indicators and conducted internal and external audits; these analyses (from 2007 to 2009) are presented herein.Lessons learned: Although there was improvement in the pre-analytical and analytical indicators analysed and although data-entry errors decreased in the post-analytical process, the delay in returning laboratory test results increased significantly. There were several factors identified as causes for this delay and all of these have now been addressed except for an identified need for automation of some high-volume assays (currently being negotiated). Internal and external audits showed a trend of increasing non-conformities which could be the result of personnel simply becoming lax over time. Application for laboratory accreditation, however, could provide the renewed vigour needed to correct these non-conformities.Recommendation: This experience shows that sustainability of the QMS at present is a cause for concern. However, the tiered system of accreditation being developed by WHO–Afro may act as a driving force to preserve the spirit of continual improvement.
Traumatismos Transmediastinales Por Arma de Fuego
Authors: Barillaro Guillermo , Saint-Genez Patricio , Gatica Sandra , Ferrer Enrique , Pomar Jose Gil 
Published: October 2012

RESUMEN
Los traumatismos transmediastinales por arma de fuego (TTMAF) plantean el desafi o de decidir rapidamente que pacientes requieren cirugia inmediata y en quienes puede realizarse un tratamiento no operatorio(TNO) El objetivo de este trabajo es evaluar el uso de un algoritmo de manejo de estas lesiones.

Métodos
Revision de historias clinicas.

Resultado
Fueron asistidos 29 pacientes con un algoritmo de diagnostico y tratamiento que incluyo dos etapas. La primer etapa con valoracion clinica, radiografi a de torax, ecografi a pericardica y control del drenaje pleural, y una segunda etapa con tomografi a computada helicoidal (TAC) y eventualmente con otros estudios. Requirieron toracotomia amplia en el ingreso 20 pacientes: 15 descompensados hemodinamicamente (13 fallecidos) y 5 normales hemodinamicamente con ecografi a con derrame pericardico o debito del drenaje pleural mayor a 1000 cc (1 fallecido). Los 9 pacientes restantes de la serie solo requirieron en el ingreso drenajes pleurales y accedieron a la segunda etapa del algoritmo con TAC mediastinal. Esta no mostro lesiones en mediastino en 4 y fue dudosa en los otros 5,en quienes se complemento con arteriografi a y endoscopia esofagica o de via aerea; estos 9 pacientes recibieron fi nalmente TNO ,con buena evolucion y sin lesiones desapercibidas.

Conclusiones
Los pacientes descompensados requirieron toracotomia amplia en el ingreso con una elevada mortalidad, mientras que dos tercios de los pacientes normales hemodinamicamente pudieron recibir TNO sin lesiones desapercibidas ni mortalidad. Este algoritmo de manejo resulto efectivo, pero requiere de disponibilidad de TAC y de todo tipo de estudios complementarios para posibilitar un TNO seguro en casos seleccionados.
Resecciones y Suturas en el Trauma Intestinal Contuso
Authors: Paredes O , Madelaire L , Domínguez J Est 
Published: October 2012

RESUMEN
En un estudio observacional retrospectivo de corte transverso de un grupo de pacientes con diagnóstico de lesión intestinal en traumatismo abdominal cerrado; operados en el Servicio de Emergencias Médicas de Asunción—Paraguay se concluyó que:

La contusión abdominal puede requerir procedimientos de sutura y-o C ya por su apertura a cavidad abdominal libre o por comprometimiento de los vasos mesentéricos.
En contusiones abdominales asociadas a lesión intestinal es más probable la lesión del intestino delgado que del colon.
La mortalidad de la serie está mayormente asociada a complicaciones de lesiones no intestinales.