bluuebunny/crossref_metadata_2025_split huggingface dataset:
PAPER INK INTERACTIONS. The effect of coating pore structure and geometry on the drying efficiency of heatset web-offset ink solvent
Authors: Gerstner Philip M. , Zhang Hai , Gane Patrick A.C. 
Published: October 2012

Abstract
This work examines the effect of coating pore structure on the evaporation behaviour of mineral oil, as a typical heatset web-offset ink solvent. Two different kinds of coating pigments are compared, a standard ground calcium carbonate (GCC) and a highly porous modified calcium carbonate (MCC) with fine intraparticle pores. The aim is to identify relevant pore structure parameters and their effect on the drying rate. Two internal stages of drying were identified for both structures : a rising drying rate period and a falling rate period. In the rising rate period, the effect of film tlow was of particular relevance for the case of GCC based structures : binder, filling the pore structure decreases the detail of geometry and thus reduces the film flow tendency, progressively depressing the drying rate. At the onset of the falling rate period the liquid phase is in the form of disconnected liquid clusters. Therefore, the diffusion resistance becomes a dominant factor. Because of the high permeability of MCC systems the evaporation rate proofed to be largely unsusceptible to the binder concentration. However, the GCC pigment systems exhibited an extended peak of the drying rate as a result of the increasing diffusion resistance with increasing binder content.
Serum Uric Acid and Its Association with Coronary Artery Disease
Authors: Akanda MAK , Choudhury KN , Ali MZ , Naher S , Islam ASME , Ali MI 
Published: October 2012

Background: Few studies have assessed the relation of uric acid level with the severity of coronary artery disease (CAD). This study investigated the association between high uric acid levels with the presence and severity of CAD.Materials and Methods: This study was designed as an observational cohort study. The study was composed of 180 patients admitted at our institution due to symptoms related to CAD. Patients having angiographic evidence of stenosis in coronary artery were as case group and without stenosis control group. Patients with high uric acid (hyperuricemia) were defined as serum uric acid concentration ?7.0mg/dl or ?420 ?mol/L in men and ?6mg/dl or ?360 ? mol/L in women. The presence of CAD has been defined as the Gensini score being ?1. Results: There was a statistically significant difference between the mean uric acid levels of patients with and without CAD (358.23±71.11 ?mol/l vs251.32±54.92 ?mol/l respectively, p<0.001). There was a statistically significant difference between ejection fraction of patients with and without CAD (54.50±9.25 vs. 63.16±6.56 respectively, p?0.001). Spearman correlation analysis demonstrated a positive correlation between the serum uric acid level and the severity of CAD (p=?0.001, r=0.39). When patients were classified into four groups according to their Gensini score, mean serum uric acid level was found to be significantly increased across the tertiles, and a statistically significant difference was detected between the tertiles (p= ?0.001). Conclusion: In conclusion, a significant association has been found between serum uric acid level and the presence and severity of CAD. In addition to the evaluation of conventional risk factors in daily clinical practice, the measurement of uric acid level might provide significant prognostic benefits in terms of global cardiovascular risk and management of the patients. DOI: http://dx.doi.org/10.3329/cardio.v5i1.12206 Cardiovasc. j. 2012; 5(1): 12-17
Extent of ST-Segment Depression Predicts In- Hospital Outcome in Non ST-Segment Elevation Acute Coronary Syndrome
Authors: Khan MR , Haq MA , Majumder NK , Hossain MM , Rahman Z , Rahman MT , Akanda AK , Mohibullah AKM 
Published: October 2012

Background: The presence of ischemic ECG changes on admission has been shown to predict outcome, the relationship between the extent of ECG changes and the risk of cardiac events is still ill defined. The severity of ST-segment depression on admission ECG has a strong association with adverse in-hospital outcome in patients with non ST-segment elevation acute coronary syndrome. The study was done to observe the extent of ST-segment depression and in-hospital outcome in patients with non ST-segment elevation acute coronary syndrome. Methods: This study was conducted in the Department of Cardiology in NICVD Dhaka, from January 2006 to December 2007. Considering inclusion and exclusion criteria, a total of 183 patients were evaluated. All the patients were evaluated clinically after admission. ECG, blood biochemistry and echocardiography were done. Patients were categorized into three groups according to the extent of ST- segment depression. Results: In this study, analysis of the baseline parameters revealed no statistically significant difference among the three groups of patients (p>0.05). Mean sum of the ST-segment depression analysis was done and all mean values were more in group III patients. 41.0% patients developed complications during the study period. Acute LVF (22.9%) was the most common complication followed by arrhythmia (11.5%), cardiogenic shock (4.4%) and STEMI (2.2%). All the complications were more in group III patients. During this period 6% patients died and more death (12.3%) was observed in group III patients. Conclusion: The amount of ST-segment depression is a powerful predictor of adverse in-hospital outcome in patients with non ST-segment elevation acute coronary syndrome DOI: http://dx.doi.org/10.3329/cardio.v5i1.12275 Cardiovasc. j. 2012; 5(1): 62-66
Digital Impression
Authors: Begum Aleya , Ahmed Rubaba , Islam Md Saiful 
Published: October 2012

The use of different impression materials is in vogue for last few years with a view of capturing more accurate dental impression, which in turn produces more accurate fitting restoration. For achieving this goal, one should have ample knowledge about the different physical and mechanical properties, as well as in different techniques with the most recent advances. In this review, we highlighted the impression material and recent updates on Digital Dentistry with the benefits which can be gained from this system by both Clinician and Dentist. DOI: http://dx.doi.org/10.3329/cdcj.v9i2.12321 City Dental College J. Volume-9, Number-2, July-2012
Supporting Ethnic Minority Families with Disabled Children: Learning from Pakistani Families
Authors: Kramer-Roy Debbie 
Published: October 2012

Introduction: This article describes how an emancipatory research approach was used to explore the support needs of Pakistani families with disabled children in the United Kingdom. These families are among the most disadvantaged in the United Kingdom and research indicates that their needs tend to remain unmet through current services. Method: A participatory action research study was undertaken with six families. After an exploratory phase of individual interviews and activities, three action research groups were formed for women, men and non-disabled siblings respectively, in which they engaged in their own research activities. This increased their insight into their situation and enabled them to gain more control. Findings: The study highlights the importance of family-centred practice when working with ethnic minority families. Key issues are continuity of intervention between school and home settings, facilitating communication within and with the family, and ensuring peer support for parents and siblings. An occupational science perspective indicates that a lack of belonging in their community affects the families' wellbeing, and that faith is an important, mostly positive, aspect of their sense of being. Conclusion: The study affirms the importance of a family-centred and occupation-centred approach when working with families from diverse ethnic minority backgrounds.
Mothering when Mothers and Children Both have Sensory Processing Challenges
Authors: Turner Karen A , Cohn Ellen S , Koomar Jane 
Published: October 2012

Introduction: The aim of this study was to understand the perspectives of mothers when mother and child both have sensory processing challenges, and how the mother's coping strategies for managing her sensory needs influence mothering co-occupations and parenting ideals. Method: Four mothers with sensory processing challenges participated in semi-structured interviews. Data were analysed using grounded theory principles. Findings: Mothers described managing their sensory needs while negotiating the conflicting sensory preferences of their children. Coping strategies included understanding sensory processing patterns of behaviour and structuring daily activities and the environment. Some coping strategies had secondary consequences of a ripple effect, a reverberating impact that exacerbated the challenges of unmet sensory needs and interfered with co-occupations and parenting ideals. Conclusion: Implications for mothers include understanding how their sensory processing transacts with their children's sensory preferences. Implications for practitioners include attending to the sensory processing patterns and coping strategies of both children and parents.
Influence of Surgical Approach on Postoperative Femoral Bone Remodelling after Cementless Total Hip Arthroplasty
Authors: Merle Christian , Sommer Julia , Streit Marcus R. , Waldstein Wenzel , Bruckner Thomas , Parsch Dominik , Aldinger Peter R. , Gotterbarm Tobias 
Published: October 2012

We retrospectively evaluated the femoral periprosthetic bone mineral density (BMD) in a consecutive series of patients who had undergone total hip arthroplasty (THA) with a straight, double tapered cementless stem using a muscle-sparing anterolateral (group A) and the transgluteal (group B) surgical approach. Dual-energy x-ray absorptiometry (DXA) measurements were performed in the first postoperative week (t1), and after 3 (t2), 6 (t3) and 12 months (t4) using an identical protocol. Patients were clinically and radiographically evaluated at final follow-up (t4). A complete set of four consecutive DXA measurements was obtained for 16 hips in group A and 26 hips in group B. In patients in whom the transgluteal approach was used (Group B), we observed a significantly greater decline in overall periprosthetic BMD (netavg) and in BMD in the periprosthetic regions of interest (ROI) 1, 4, 5 and 6 between t1 and t4. At clinical and radiographic evaluation at t4, no differences between the groups were detected. Femoral periprosthetic BMD is affected by the selected surgical approach in the first postoperative year. This might be attributed to altered femoral loading as a result of differences in intraoperative damage to the abductor muscles.
Two-Year Radiologic Assessment of the Trident Peripheral Self-Locking Cup Using Ebra
Authors: Nunag Perrico , Deakin Angela H. , Oburu Ezekiel , Sarungi Martin 
Published: October 2012

We performed a radiologic assessment of the Trident Peripheral Self-Locking cup 2 years after implantation to assess early migration behaviour and to establish if incomplete postoperative seating correlated with early instability. A retrospective analysis of 30 cases was performed using EBRA. No cups had acetabular screws. Average total migration was 1.5 mm (range 0.1 to 5.9 mm). Seventeen showed total migration >1 mm and 7 of these showed further migration >2 mm (range 2.3 to 5.9 mm). Twenty cups demonstrated incomplete seating on initial post-operative radiographs (mean 1.4 mm, range 0.3 to 3.0). No relationship between incomplete seating and migration was identified (p = 0.86). The majority of gaps consolidated at differing times within the 2 year period. Oxford Hip scores showed significant improvement after surgery (p = 0.001) and this was independent of migration (p = 0.76). At 5 years there were no revisions for aseptic loosening. Conclusion The majority of the cups demonstrated early radiographic instability, and this was not related to incomplete seating. Five year functional outcome appears good and independent of migration and initial seating.
New Tween-80 Microbiological Assay of Serum Folate Levels in Humans and Animals
Authors: Zhou Zhenghua , Yang Yuan , Li Ming , Kou Chong , Xiao Ping , Jiang Yan , Hong Junrong , Huang Chengyu 
Published: October 2012

Abstract
The objective of this study was to develop a new Tween-80 microbiological assay (Tween-80 MBA) to determine human or animal serum folate levels and to verify its reliability. The effects of the Lactobacillius casei subspecies rhamnosus (L. casei, ATCC No. 7469) inoculum concentration, incubation time, and Tween-80 on L. casei growth were studied, and the serum folate levels were investigated. Serum samples were collected from patients with esophageal cancer (EC) and healthy control subjects in Yanting, healthy adult subjects in Chengdu, Sichuan, and in male Sprague-Dawley rats. Optimal conditions for the new MBA were as follows: 1.28 × 107 CFU/mL working inoculum, vitamin folic acid assay broth with 0.24% (w/w) Tween-80, and anaerobic incubation with L. casei at 37°C for 22 h. Under the optimal conditions, the working curve was in simple linear rather than logarithmic equation; the linear working curve of the folic acid standard working solution concentration versus the turbidity (adsorption value) of medium with L. casei ranged from 0.05 to 1.00 μg/L; the linear correlation coefficient was 0.9989 (SD 0.0007); the recovery rate of folate was 105.4–112.7%; and the minimum concentration for detecting folate was 0.03 μg/L. The RSD within-day and between-day precisions were 5.6 and 3.3%, respectively. The serum folate level of 100 EC patients was 6.4 (SEM 0.4) μg/L which was significantly lower than that of healthy control subjects [8.0 (SEM 0.6) μg/L, n = 100, P = 0.020]. The new Tween-80 MBA is considered to be a reliable method for measuring serum folate level.
Validation of the Applied Biosystems MicroSEQ® Real-Time PCR System for Detection of E. coli O157:H7 in Food
Authors: Wong Lily Y , Cao Yanxiang , Balachandran Priya , Zoder Patrick , Furtado Manohar R , Petrauskene Olga V , Tebbs Robert S 
Published: October 2012

Abstract
Modern molecular methods offer the advantages of simplicity and short time-to-results compared to traditional culture methods. We describe the validation of a new Real-Time PCR method to detect E. coli O157:H7 in five food matrixes. The complete system consists of the MicroSEQ®E. coli O157:H7 Detection Kit, sample preparation (two sample preparation methods, the PrepSEQ® Nucleic Acid Extraction Kit and the PrepSEQ Rapid Spin Sample Preparation Kit, were validated), the Applied Biosystems 7500 Fast Real-Time PCR instrument, and RapidFinder™ Express software. The test method was compared to the U.S. Department of Agriculture Microbiology Laboratory Guidebook 5.04 reference method for detecting E. coli O157:H7 in 25 g and 375 g ground beef and beef trim, and to the ISO 16654 reference method for detecting E. coli O157:H7 in 25 g spinach, orange juice, and apple juice. The MicroSEQ E. coli O157:H7 Detection Kit showed equivalent detection compared to the corresponding reference method based on Mantel-Haenszel Chi-square statistics for all matrixes tested. An independent validation confirmed these findings on ground beef. The MicroSEQ kit detected all 51 E. coli O157:H7 strains tested and showed good discrimination against an exclusivity panel of 30 strains.
Optimization and Validation of a Simple and Fast Method for the Determination of Fungicides in Must and Wine Samples by SPE and GC/MS
Authors: Lagunas-Allué Laura , Sanz-Asensio Jesús , Martínez-Soria Maria-Teresa 
Published: October 2012

Abstract
A rapid, simple, and low-cost method based on SPE was optimized and validated for simultaneous determination of eight fungicides belonging to different chemical classes in must and wine. The method involves extraction of 10 mL of must or wine samples with a C18 cartridge using 5 mL of dichloromethane as the elution solvent. Separation and final determination of the fungicides (vinclozolin, dichlofluanid, penconazol, captan, quinoxyfen, fluquinconazol, boscalid, and pyraclostrobin) was performed by GC coupled to single quadrupole MS. Recoveries at 10, 50, and 100 μg/L were between 71 and 106% in both matrixes for the fungicides evaluated. The calculated LOQ ranged from 1.5 to 3.4 μg/L in must and 1.1 to 3.8 μg/L in wine. Matrix effects observed for wine and must samples were overcome by using matrix-matched calibration. The developed method was linear at concentrations within the tested interval, with coefficients of determination higher than 0.999. The expanded uncertainties at 10 μg/L were &lt;20% for all analytes. Intralaboratory precision in terms of the Horwitz ratio of the fungicides evaluated was below 0.5, suggesting the ruggedness of the method. The proposed method was applied to determine fungicide residues in must samples obtained from red grapes treated with two new commercial formulations, as well as in their corresponding final wines.
Evaluating the Use of Fatty Acid Profiles to Differentiate Human Pathogenic and Nonpathogenic Listeria Species
Authors: Whittaker Paul 
Published: October 2012

Abstract
Listeria monocytogenes is a Gram-positive human pathogen that is responsible for serious infections in immunocompromised individuals and pregnant women. Because of recent epidemics caused by food contaminated with L. monocytogenes, rapid methods for the detection of this pathogen in food are of interest. Capillary gas chromatography with flame ionization detection (GC-FID) was used to determine the cellular fatty acid profiles of six species of Listeria. The six different species are L. monocytogenes, L. ivanovii, L. innocua, L. welshimeri, L. seeligeri, and L. grayi. For GC-FID analysis, whole cell fatty acid methyl esters (FAMEs) from cells cultured on brain heart infusion (BHI) agar at 35 ° C for 24 h were obtained by saponification, methylation, and extraction into hexane/methyl tert-butyl ether. A preliminary data set for 15 strains of Listeria species was prepared using fatty acid profiles from two or three replicates prepared on different days. Major fatty acids of the Listeria strains evaluated in this study were C15:0 iso, C15:0 ante iso, C16:0 iso, C16:0, C17:0 iso, and C17:0 ante iso. All of the major fatty acids differ significantly among these six species. The two fatty acids C17:0 ante iso and C15:0 ante iso showed the highest percentages, and the ratio of the two clearly showed significant differences between the human pathogen L. monocytogenes and the five nonpathogenic species. Analysis of FAMEs from Listeria strains grown on BHI agar by a GC-FID method is a sensitive procedure for identification of these organisms and differentiation between pathogenic and nonpathogenic species.
Quantification of Vitamin D3 in Feed, Food, and Pharmaceuticals Using High-Performance Liquid Chromatography/Tandem Mass Spectrometry
Authors: Schadt Heiko S , Gössl Richard , Seibel Natalie , Aebischer Claude-P 
Published: October 2012

Abstract
A rapid, sensitive, and selective method for the quantification of vitamin D3 (cholecalciferol) in solid and liquid food, feed, and tablets based on HPLC/MS/MS has been developed and validated. The sample preparation procedure consists of a quick and robust alkaline saponification and liquid–liquid extraction, followed by direct injection of the organic extract into the HPLC/MS/MS system for analysis without any further concentration, reconstitution, or prepurification steps. The reduction in sample preparation time was achieved by applying a heart-cutting, two-dimensional chromatography technique prior to positive electrospray ionization selected reaction monitoring MS analysis. Total vitamin D3 (sum of previtamin D3 and vitamin D3) was quantified using an isotopically labeled internal standard. The ionization efficiency of previtamin D3 and vitamin D3 in the positive electrospray ionization mode was found to be very similar. The validation experiments included four feed matrixes, three types of tablets, and 12 food matrixes. The obtained recoveries were between 96.1 and 105.3%, and intermediate precision ranged from 1.32 to 15.6% RSD, with HorRat values between 0.07 and 0.65. For all samples, extraction efficiencies were above 95.8%. Analysis of two certified reference materials (SRM 1849 and BCR-122) gave accuracies of 102.4 and 99.8%, respectively.
Validation of the Legipid® Bioalarm Legionella Assay
Authors: Albalat Guillermo Rodríguez , Broch Begoña Bedrina , Bono Marisa Jiménez 
Published: October 2012

Abstract
Legipid® Bioalarm Legionella is a test based on combined magnetic immunocapture and enzyme-immunoassay (CEIA) for the detection of Legionella pneumophila in water. Anti-L. pneumophila antibodies are immobilized on magnetic microspheres. Immunomagnetic analysis is applied to preconcentrated water samples in a final test volume of 9 mL. The method was compared with the standard culture method on both spiked and naturally contaminated water samples. The test was evaluated in potable, industrial, and natural water matrixes, according to the scope of the ISO 11731 reference method. These waters were tested with the target at levels ranging from low (10–99 CFU/mL) to high (100–999 CFU/mL); a Chi-square value of 1.8 indicated that there was no significant difference between the test and the reference method. The false-positive rate was 7%, and the false-negative rate 2%. For the inclusivity study, all 17 strains of L. pneumophila of different serogroups reacted with the test. For the exclusivity study, 17 strains of other Legionella species and 16 non-Legionella strains were tested. There were no cross-reactions with non-Legionella strains. L. beliardensis, L. adelaidensis, and one environmentally isolated Legionella sp. produced a positive result at high concentrations of 1800, 230, and 3900 CFU/mL, respectively. Agreement between the two methods was 95.9%.
Determination and Confirmation of Parent and Total Ractopamine in Bovine, Swine, and Turkey Tissues by Liquid Chromatography with Tandem Mass Spectrometry: First Action 2011.23
Authors: Burnett Thomas J , Rodewald John M , Brunelle Sharon L , Neeley Mark , Wallace Michael , Connolly Paul , Coleman Mark R 
Published: October 2012

Abstract
A candidate method selected by the AOAC Expert Review Panel (ERP) for Ractopamine for determination and confirmation of parent and total ractopamine by LC/MS/MS was validated in a single laboratory for bovine, swine, and turkey tissues. The candidate method utilizes methanol extraction of the tissues, followed by an optional enzymatic hydrolysis for determination of total (parent plus conjugate) ractopamine. A mixed-mode cation exchange SPE cartridge is used to purify the initial extract before LC/MS/MS. Matrix-matched standards and a ractopamine-d6 internal standard are used for quantification of parent and total ractopamine in unknown samples. Validation data demonstrated that mean intertrial recoveries for ractopamine across all concentrations tested ranged from 79.7 to 102.2% for parent ractopamine and from 79.0 to 100.0% when a hydrolysis step was included. Intertrial repeatability precision ranged from 2.44 to 11.1% for parent ractopamine and 4.97 to 15.0% with hydrolysis. Estimated LOD values were below 0.1 ng/g and LOQ values were validated at 0.25x the maximum residue limits. The data satisfy the requirements of the AOAC Stakeholder Panel for Veterinary Drug Residue Methods for single laboratory validation studies. The method was awarded Official Methods of AnalysisSM First Action 2011.23 by the AOAC ERP on Veterinary Drug Residues.