bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microcomputed Tomography and Histologic Analysis of a Maxillary Implant Retrieved 19 Months After Placement and Indirect Sinus Augmentation With Biphasic Calcium Phosphate: A Case Report
Authors: Verket Anders , Lyngstadaas S. Petter , Rønold Hans J. , Wohlfahrt J. Caspar 
Published: October 2012

Introduction: Implant osseointegration in regenerated bone and the resorptive outcome of graft materials are major clinical concerns.Case Presentation: A 40‐year‐old female patient underwent an indirect maxillary sinus augmentation with biphasic calcium phosphate (BCP) in conjunction with implant placement. Because of implant fracture, the implant and adjacent tissues were excised with a trephine bur 19 months after placement. The biopsy with the implant in place was analyzed by microcomputed tomography (micro‐CT) and histology. Marginal bone loss of the implant was evident, particularly on the fractured side. The graft particles were predominantly integrated in bone. Resorption lacunae were observed in the marginal bone area, but were not observed on the surface of the graft particles. Graft particles without contact to bone were observed coronal to the most coronal level of bone‐to‐implant contact, presumably exfoliated after marginal bone resorption. Of the total peri‐implant volume, 20.5% was residual graft material and 62.5% was bone according to the micro‐CT analysis. The histomorphometry demonstrated 30.4% residual graft material and 46.8% bone of the total peri‐implant area. The percentage of bone‐to‐implant contact was 60.3% when counting the total vertical implant perimeter.Conclusions: The BCP particles were mostly embedded in new bone, and the implant was clinically and histologically osseointegrated. This case report suggests that BCP graft material may not resorb during inflammation, resulting in exfoliation from the grafted compartment.
Progression of Osteogenic Cell Cultures Grown on Microtopographic Titanium Coated With Calcium Phosphate and Functionalized With a Type I Collagen‐Derived Peptide
Authors: Pereira Karina K.Y. , Alves Olívia C. , Novaes Arthur B. , de Oliveira Fabíola S. , Yi Ji‐Hyun , Zaniquelli Osvaldo , Wolf‐Brandstetter Cornelia , Scharnweber Dieter , Variola Fabio , Nanci Antonio , Rosa Adalberto L. , de Oliveira Paulo T. 
Published: October 2012

Background: The functionalization of metallic surfaces aims at promoting the cellular response at the biomaterial–tissue interface. This study investigates the effects of the functionalization of titanium (Ti) microtopography with a calcium phosphate (CaP) coating with and without peptide 15 (P‐15), a synthetic peptide analog of the cell‐binding domain of collagen I, on the in vitro progression of osteogenic cells.Methods: Sandblasting and acid etching (SBAE; control) Ti microtopography was coated with CaP, enabling the loading of two concentrations of P‐15: 20 or 200 μg/mL. A machined Ti was also examined. Rat calvarial osteogenic cells were cultured on Ti disks with the surfaces mentioned above for periods up to 21 days (n = 180 per group).Results: CaP coating exhibited a submicron‐scale needle‐shaped structure. Although all surfaces were hydrophobic at time zero, functionalization increased hydrophilicity at equilibrium. Microtopographies exhibited a lower proportion of well‐spread cells at 4 hours of culture and cells with long cytoplasmic extensions at day 3; modified SBAE supported higher cell viability and larger extracellular osteopontin (OPN) accumulation. For SBAE and modified SBAE, real‐time polymerase chain reaction showed the following results: 1) lower levels for runt‐related transcription factor 2 at 7 days and for bone sialoprotein at days 7 and 10 as well as higher OPN levels at days 7 and 10 compared to machined Ti; and 2) higher alkaline phosphatase levels at day 10 compared to day 7. At 14 and 21 days, modified SBAE supported higher proportions of red‐dye‐stained areas (calcium content).Conclusion: Addition of a CaP coating to SBAE Ti by itself may affect key events of in vitro osteogenesis, ultimately resulting in enhanced matrix mineralization; additional P‐15 functionalization has only limited synergistic effects.
Plasma Rich in Growth Factors Promotes Bone Tissue Regeneration by Stimulating Proliferation, Migration, and Autocrine Secretion in Primary Human Osteoblasts
Authors: Anitua Eduardo , Tejero Ricardo , Zalduendo Mari Mar , Orive Gorka 
Published: October 2012

Background:Alveolar bone loss can be a major clinical concern affecting both functionality and esthetics. Osteoblasts are the main cells charged with the repair and regeneration of missing bone tissue. Plasma rich in growth factors (PRGF) allows delivery of a cocktail of proteins and growth factors that promote wound healing and tissue regeneration to the site of injury. This study tests the effect of this endogenous regenerative technology to stimulate alveolar osteoblast bone‐forming potential.Methods:Primary human osteoblasts were retrieved from alveolar bone of patients undergoing oral surgery. Cell proliferation was evaluated, and culture inserts and permeable transwell supports were used to assess cell migration and chemotaxis. The expression of differentiation markers was quantified by enzyme‐linked immunosorbent assay.Results:PRGF succeeded in increasing proliferation, migration, and chemotaxis of osteoblasts. Also, PRGF significantly enhanced the autocrine expression of two relevant proangiogenic factors, vascular endothelial growth factor and hepatocyte growth factor, and three markers of osteoblastic activity, procollagen I, osteocalcin, and alkaline phosphatase.Conclusion:The results indicate that PRGF can stimulate some of the biologic processes of the main cells responsible for bone regeneration and help support the positive clinical outcomes that have been reported with this technology.
Use of a Collagen Membrane Loaded With Recombinant Human Bone Morphogenetic Protein‐2 With Collagen‐Binding Domain for Vertical Guided Bone Regeneration
Authors: Lai Chun‐Hua , Zhou Lei , Wang Zhong‐Lei , Lu Hai‐Bin , Gao Yan 
Published: October 2012

Background: Vertical bone regeneration of severe atrophic alveolar ridges remains a challenging procedure in implant dentistry.Methods: The aim of this study, accordingly, is to use a rabbit vertical guided bone regeneration model to evaluate whether using a collagen membrane (CM) loaded with small doses of recombinant human bone morphogenetic protein‐2 with collagen‐binding domain (rhBMP‐2/CBD) would enhance two‐way vertical bone regeneration. In each of eight rabbits, four titanium cylinders were screwed in perforated slits made into the external cortical bones of the calvaria. The following four treatment modalities were randomly allocated: 1) cylinders filled with mineralized bone matrix and covered with CM/rhBMP‐2/CBD; 2) cylinders filled with mineralized bone matrix and covered with CM/rhBMP‐2; 3) cylinders filled with mineralized bone matrix and covered with CM alone; or 4) cylinders filled with mineralized bone matrix without a membrane cover.Results: After 6 weeks, the new bones were examined by histologic analysis. Slender new bone trabeculae were observed in the superficial layer of the titanium cylinders covered with CM/rhBMP‐2/CBD, and higher degrees of bone were observed in this group compared with the other three groups. The average area fraction of newly formed bone was significantly more in the CM/rhBMP‐2/CBD group compared with the CM/rhBMP‐2, CM, or the no membrane control groups (all P <0.01).Conclusions: The present study demonstrates that CMs loaded with small doses of rhBMP‐2/CBD induce new bone formation not only from the surface of the native bone, but also from the superficial structures. The augmented new bone, therefore, is improved in both quantity and quality.
The Use of Bur and Laser for Root Caries Treatment: A Comparative Study
Authors: Geraldo-Martins V , Thome T , Mayer M , Marques M 
Published: October 2012

Summary
This research analyzed the influence of bur and erbium, chromium:yttrium-scandium-gallium-garnet (Er,Cr:YSGG) laser caries removal on cavity characteristics and marginal seal of composite resin restorations. One hundred and forty human dental root samples were used. After in vitro root caries induction using Streptococcus mutans, the carious lesions were removed either by a conventional technique using burs (G1=control) or by using an Er,Cr:YSGG laser (λ=2.78 μm, 20 Hz, pulse duration≅140 μs, noncontact mode using a 600-μm tip) with the following power outputs: G2: 1.0 W; G3: 1.25 W; G4: 1.5 W; G5: 1.75 W; G6: 2.0 W; G7: 2.25 W; G8: 2.5 W; G9: 2.75 W; G10: 3.0 W; G11: 3.25 W; G12: 3.5 W; G13: 3.75 W; and G14: 4.0 W. Samples in the 14 groups (n=10) were conditioned with Clearfil SE Bond and restored with a flowable composite. They were then thermocycled (1000 cycles) and immersed into a 2% methylene blue solution for microleakage analysis. The data were statistically compared (analysis of variance or Spearman correlation tests; p≤0.05). The lased groups showed significantly greater microleakage indexes, cavity depths, and presence of residual caries than did those of the control group. There was a strong positive correlation between residual caries and microleakage. The results indicate that Er,Cr:YSGG laser irradiation is not a good alternative to the use of burs for root caries removal since it may cause a significant loss of marginal sealing in composite resin restorations.
Effect of Using Silorane-based Resin Composite for Restoring Conservative Cavities on the Changes in Cuspal Deflection
Authors: Shabayek NM , Hassan FM , Mobarak EH 
Published: October 2012

SUMMARY

Objective:
To investigate the effect of using two resin-composite materials for restoring conservative mesio-occluso-distal (MOD) cavities on the changes (incremental and cumulative) in cuspal deflection.

Methods:
Forty extracted sound human maxillary second premolars were subjected to standardized MOD cavity preparation and then divided into two groups (n=20). The first group of teeth was restored with Filtek Z250 (3M ESPE, St Paul, MN, USA), and Filtek P90 (3M ESPE, St Paul, MN, USA) was used in the second group. Incremental cuspal deflection was calculated by measuring the intercuspal distance between the indexed cusp tips before the restoration and at five-minute intervals up to 30 minutes using a stereomicroscope connected to a digital camera. Cumulative cuspal deflection for both materials was also calculated.

Results:
Comparing the incremental cuspal deflection of the tested groups at each time interval, it was found that there was no significant difference immediately after curing and at five, 15, 20, and 25 minutes. However, a significant difference was recorded at 10 and 30 minutes. For the cumulative cuspal deflection, Filtek P90 showed significantly lower deflection values than Filtek Z250 only after five minutes.

Conclusions:
Incremental cuspal deflections of both materials over the tested intervals were almost comparable. However, after five minutes of curing, silorane-based resin composite surpassed the methacrylate-based resin composite in controlling the cumulative cuspal deflection.
Lateral Tibial Plateau Fracture
Authors: Flautt Warren , Miller Joseph , Ratcliff Jennifer Robyn 
Published: October 2012

The patient was a 30-year-old man who was initially seen by a physical therapist for a chief complaint of left knee pain. Due to concern for a fracture, the physical therapist ordered radiographs of the left knee, which revealed a fracture of the left lateral tibial plateau. J Orthop Sports Phys Ther 2012;42(9):819. doi:10.2519/jospt.2012.0416
DMA Clinical Pilates Directional-Bias Assessment: Reliability and Predictive Validity
Authors: Tulloch Evelyn , Phillips Craig , Sole Gisela , Carman Allan , Abbott J. Haxby 
Published: October 2012

Study Design Randomized, repeated-measures crossover design. Objectives To determine the interrater reliability of directional-bias assessment and to investigate its validity for predicting immediate changes in dynamic postural stability and muscle performance following directionally biased exercises. Background Directional bias in dynamic postural stability deficits may be associated with outcome following intervention. Methods Two researchers independently assessed 33 participants, each with a history of more than 1 unilateral lower-limb injury, for directional bias. Interrater reliability was evaluated with the kappa coefficient and a prevalence-adjusted and bias-adjusted kappa coefficient. Participants were randomly allocated to perform matched-bias (MB) or unmatched-bias (UB) exercises first, in 2 crossover groups. Two outcome measures, time to stabilization and rebound hopping, were assessed before and following each exercise intervention, using a force plate. Crossover trial data were analyzed by t tests for period, interaction, and treatment effects, and repeated-measures analyses of variance were used to investigate differences between baseline, MB, and UB. Results Interrater reliability of directional-bias assessment was substantial (κ = 0.75; prevalence-adjusted and bias-adjusted κ = 0.76). Following MB exercises, medial/lateral time to stabilization and time on the ground during rebound hopping were significantly shorter (P = .01 and P = .05, respectively) compared with UB exercises. Compared with baseline, pairwise change in anterior/posterior time to stabilization (P = .008) improved following MB, whereas time in the air decreased following UB (P = .036). Conclusion Directional-bias assessment demonstrates substantial reliability, and outcomes suggest validity for predicting immediate improvements following matched directionally biased exercises. J Orthop Sports Phys Ther 2012;42(8):676–687. doi:10.2519/jospt.2012.3790
Electromechanical Delay of the Vastus Medialis Obliquus and Vastus Lateralis in Individuals With Patellofemoral Pain Syndrome
Authors: Chen Han-Yu , Chien Chia-Chen , Wu Shyi-Kuen , Liau Jiann-Jong , Jan Mei-Hwa 
Published: October 2012

Study Design Case-control study. Objective To examine electromechanical delay (EMD) of the vastus medialis obliquus (VMO) and the vastus lateralis (VL) in individuals with patellofemoral pain syndrome (PFPS). Background EMD is a mechanical property of muscles related to protective reflex and sports performance. The time duration of the EMD can be shortened with strength training and, conversely, can be lengthened secondary to immobilization. However, it is unclear if EMD between various components of the quadriceps is affected in individuals with PFPS. Methods Twenty-six individuals with PFPS and 26 healthy volunteers were studied. The VMO and VL were electrically stimulated to evoke muscle twitches. Ultrasound was used to assess patellar movement elicited by the muscle twitch. The time from the onset of electrical stimulation to the onset of patellar movement was measured as the EMD. The EMDs of the VMO and VL were compared between groups using a mixed-model analysis of variance. Results Subsequent to a significant interaction (P<.001), post hoc analysis indicated that the EMD of the VMO was longer (PFPS, 37.3 ± 0.7 milliseconds; control, 25.9 ± 0.7 milliseconds; P<.001) and the EMD of the VL was shorter (PFPS, 18.4 ± 0.5 milliseconds; control, 25.1 ± 0.5 milliseconds; P<.001) in the PFPS group. Therefore, in the individuals with PFPS, the EMD of the VMO was significantly longer than that of the VL (P<.001), which was not the case for those in the control group (P = .20). Conclusion The mechanical properties of the VMO and VL may be altered in patients with PFPS. J Orthop Sports Phys Ther 2012;42(9):791–796, Epub 2 August 2012. doi:10.2519/jospt.2012.3973
Patient Age Is Related to the Types of Physical Therapy Interventions Provided for Chronic Low Back Pain: An Observational Study
Authors: Greenberger Hilary B. , Beissner Katherine , Jewell Dianne V. 
Published: October 2012

Study Design Retrospective analysis of a longitudinal cohort of patients treated for chronic low back pain (CLBP). Objectives To determine whether patient age is associated with types of physical therapy interventions received for CLBP. Background Advancing age is associated with less positive treatment outcomes in patients with CLBP. If patient age influences a therapist's choice of interventions, it may partially explain the difference in treatment outcomes. Methods Data were examined in a sample of 7392 patients (62% women, 38% men; mean ± SD age, 56.7 ± 16.5 years) with CLBP. We used a generalized estimating equation to examine the probability of each subject receiving each of the individual intervention categories in the presence of the other intervention categories. Results A significant interaction between intervention category and age existed after controlling for gender, duration of symptoms, comorbidities, payer source, and functional status at initial intake (χ2 = 130.27, df = 8, P<.0001). The changes in probability of receiving an intervention category averaged 10% as patient age increased. The probability of receiving exercise or task-specific training did not change with advancing age. The probability of receiving postural exercises, pain modalities, joint mobility techniques, ice, or McKenzie exercises decreased with advancing age. The probability of receiving augmented soft tissue mobilization and balance/mobility training increased as age increased. Conclusion The impact of age on physical therapist treatment choices varies depending on the type of intervention. Other variables in addition to age may have an impact on treatment choice. Further research is needed to determine how therapists incorporate age into their clinical decision making. J Orthop Sports Phys Ther 2012;42(11):902–911, Epub 21 September 2012. doi:10.2519/jospt.2012.4147
Multiple/Overlapping Stents in a Single Artery Territory in Bangladeshi Patient Population with Diabetes Mellitus (DM)-Sirolimus Eluting Stent (Cypher) shows better Patency and Reduced ISR: A Single Center Experience
Authors: Islam AHMW , Munwar S , Talukder S , Reza A , Ahmed T , Bhuiyan A , Siddique AB 
Published: October 2012

Background: Data of stent patency or the development of ISR in multiple or overlapping stent in a single vessel territory of Bangladeshi diabetic (DM) patient population is not yet available. Therefore, the aim of our present study was to assess primarily the long-term stent patency of varieties of stent in patient with Diabetes Mellitus (DM) and Non-diabetes Mellitus (NDM).Methods: Patients were prospectively selected from, who underwent coronary angiogram at our hospital for further evaluation of their previous PTCA in the e36 months preceding the study for the quantifying period of 2007-2011. Total 51 (11%) patients (male: 41, Female: 10) from a total 461 patients were included in this study who has multiple or overlapping stent in single vessel territory. Total 32 (62.7%) patients included into the DM group with an average age of 57 yrs and in the NDM were 19 (37.3%) with 58 yrs. Average ISR period was 3.1 ± 2.4 yrs. Results: Our result shows that total 70 stent were deployed in 33 vessels of DM patient and 44 stent in 21 vessel of Non-DM patient. Territory wise total number of deployed stent in patient of DM: NDM were in LAD 34 (48.6%): 18(41%), RCA 16 (22.8%): 4 (9%) and LCX 20(28.6%): 22(55%). Stent used in DM: NDM; were BMS 21 (30%): 22(50%), Sirolimus 24(34.3%): 10(22.7%), Paclitaxel 17(24.3%):8(18.2%), Everolimus 6(8.6%):4(9.1%)%). Re-look coronary angiogram (CAG) revealed that increased number of in-stent restenosis occurred more in patient with Diabetes [DM 10(31.3%) vs NDM 5(26.3%)]. Significant ISR (ISR>60%) in DM patient treated with for BMS were 4 (36.4%), for Paclitaxel 3(42.3%), for Sirolimus 1(9.1%). Average stent size and length were almost same in both groups for any coronary territory.Conclusion: Our study has revealed that increased number of ISR were more in patient with diabetes (DM) in a single artery territory when multiple stents were used either separately or as overlapping. Among the stent used, Sirolimus shows better patency with reduced ISR than Paclitaxel and other limus eluting stent for an average period of follow up of 3.1 yrs. DOI: http://dx.doi.org/10.3329/cardio.v5i1.12208 Cardiovasc. j. 2012; 5(1): 18-22
Antibiotic Sensitivity Pattern of Bacterial Isolates from Different Clinical Specimens: Experience at NICVD, Dhaka
Authors: Shahidullah MS , Yusuf MA , Khatun Z , Ara UKMN , Mitul MT 
Published: October 2012

Background: Infectious diseases are among the leading causes of death and sometimes curable. Bacteria are the most common etiology in hospitalized patients. The objective of this study was to evaluate the incidence of bacterial infections and their pattern of susceptibility to antibiotics in moderate and severe infections in patients admitted at NICVD hospital.Methodology: The study was performed in the apical teaching hospital of Bangladesh situated at Dhaka in the first half of 2012. Patients admitted in medical wards and medical ICU, suffering from moderate and severe infections were studied. Clinical evaluation, routine and specific investigations were done in each case. Microbiological samplings were tried on day 1, after completion of antibiotic therapy or in between as required. Aerobic bacterial culture and sensitivity tests were done. Result: A total of 274 cases were studied of which male (67.5%) were predominant than female (32.5%). The highest number of patients were in the age group of 30-60 years (53.6%) followed by 10- 30 years (24.5%) and more than 60 years (12.8%). The mean age with standard deviation was 39.15±19.07 years (range 1-90 years). Most common isolated bacteria was the Pseudomonas species (14.2%) followed by Escherichia coli (13.5%), Staphylococcus saprophyticus (6.9%) and Staphylococcus aureus (2.2%). From these specimens Pseudomonas species (31.5%) was isolated mostly from pus. E. coli was found most commonly in pus (16.3%) and urine (14.1%). Staph saprophyticus (13.0%) and Staphylococcus aureus (4.3%) were both found most commonly in pus. Pseudomonas species was 100% resistant to Penicillin, Amoxycillin and Vancomycin. It was found that Pseudomonas species was still more than 90% sensitive only to Imipenem. Escherichia coli was more than 80% sensitive to only Imipenem and Amikacin. Staphylococcus aureus was sensitive to only Imipenem and Cephalexin. Conclusion: In this study Pseudomonas species and Escherichia coli are the most common isolated bacteria in this Institution. Most of the antibiotics are resistant to these two bacteria. DOI: http://dx.doi.org/10.3329/cardio.v5i1.12276 Cardiovasc. j. 2012; 5(1): 67-72