bluuebunny/crossref_metadata_2025_split huggingface dataset:
Keratoma Hereditaria Mutilans (Vohwinkel's Disease) with Congenital Alopecia Universalis (Atrichia Congenita)
Authors: Bhatia Krishan Kumar , Chaudhary Satdev , Pahwa Ujjal Singh , Mehrotra Girish Chander 
Published: April 2015

AbstractA family is described in which a boy and two girls had features of keratoma hereditaria mutilans (Vohwinkel's disease) and congenital absence of hair. None of them had any hair at birth or developed any subsequently. They had congenital, nonscarring alopecia universalis. The skin over the palms and soles showed progressive thickening, which led to mutilating deformities of the hands and fingers. Histopathological examination of the scalp skin revealed no changes in the epidermis or dermis with normal sebaceous glands and identificable hair structures; the skin on the palms showed a tremendously thickened horny layer. Both conditions remained unresponsive to various topical and systemic remedies used in the past. One of the girls had a meningocoele of the dorsolumbar region and died postoperatively. The simultaneous appearance of two rare hereditary diseases in siblings of one family is being reported for the first time.
Characteristics of Cytokeratins from Stratum Corneum of Palms
Authors: Shimoura Takako , Hozumi Yutaka , Aso Kazuo 
Published: April 2015

AbstractWe have examined cytokeratins from stratum corneum (s. corneum) of the palm and back of hands by biochemical techniques which characterize cytokeratin No. 9 (CK9).By two‐dimensional gel electrophoresis, cytokeratins from s. corneum of the palm (PSC) were resolved into three spots (designated as CK‐A, CK‐B and CK‐C by us), and cytokeratins from s. corneum of the back of hands (BSC) were resolved into two spots (CK‐A and CK‐C). The results of polypeptide mapping and the spot positions in two‐dimensional gels suggest that CK‐A, CK‐B and CK‐C are degraded products derived from CK1/2, CK9 and CK10/11 in living keratinocytes of epidermis, respectively. While the spots of CK‐A and CK‐B were prominent and the spot of CK‐C was very weak in PSC, the spots of CK‐A and CK‐C were prominent and the spot of CK‐B was not detected in BSC.In filament reconstitution in vitro, cytokeratins from PSC formed intermediate‐sized filaments similar to those commonly observed using cytokeratins from whole epidermis. In contrast, cytokeratins from BSC formed only short filaments and small granules. In order to examine this difference in detail, polypeptides were eluted electrophoretically from gel slices containing spots. Addition of eluted CK‐B to cytokeratins from BSC promoted the formation of intermediate‐sized filaments similar to those from PSC. Furthermore, although the mixture of eluted CK‐A and CK‐C did not form intermediate‐sized filaments, the combination of CK‐A and CK‐B alone did form them.These results show that, as the acidic partners to the basic CK‐A in filament reconstitution, CK‐B (CK9) are more efficient than CK‐C (CK10/11).
Flow Cytometric Analysis of Murine Splenic Cells after DNFB Painting on Langerhans Cell Deficient Skin
Authors: Morita Hideki , Kihara Takako , Kuramoto Masaru , Minami Shoichiro , Yu Masaki , Yamagata Masaharu , Sagami Seiichiro 
Published: April 2015

AbstractOne week after a single painting of 50 μ1 of 0.5% DNFB in acetone‐olive oil on the tail skin of C3H mice, the spleens were removed from the animals. A single cell suspension was prepared from the spleens, and flow cytometric analysis was performed for L3T4 and Lyt2 positive cells, as well as for the IJk expression of the Lyt2+ cells.Results showed that the percentages and absolute numbers of Lyt2+ L3T4– cells, Lyt2– L3T4+ cells, Lyt2+ IJk+ cells, and Lyt2+ IJk– cells in the spleens of the DNFB‐treated mice were not significantly different from those in the non‐treated mice. However, in the treated mice, the IJk expression of Lyt2+ cells intensified. These results indicate that, following a single painting of DNFB onto Langerhans cell‐deficient skin, the numbers of Lyt2+ cells do not change significantly, but do change functionally.
A Case of Bullous Pemphigoid Induced by Tiobutarit (D‐penicillamine Analogue)
Authors: Yamaguchi Reiko , Oryu Fuyuki , Hidano Akira 
Published: April 2015

AbstractA 61‐year‐old woman with severe rheumatoid arthritis developed bullous lesions on the trunk, conjunctiva, and oral mucosa during treatment with tiobutarit, a new antirheumatic drug analogus to D‐penicillamine. Histology showed a subepidermal blister, and circulating antibodies to the basement membrane zone were detected. The bullous lesions improved rapidly after discontinuation of tiobutarit. However, 3 years later, she was re‐exposed to tiobutarit and the bullous lesions recurred within a week. This is the first confirmed case of tiobutarit‐induced BP.
Flow Cytometric Analysis of Protein Kinase C in Lymph Node Cells during the Induction Phase of Contact Hypersensitivity Reaction
Authors: Morita Hideki , Yamagata Masaharu , Inohara Shinichi , Sagami Seiichiro 
Published: April 2015

AbstractOn the fourth day after a single painting of 2,4,6‐trinitro‐chlorobenzene on the abdominal skin, inguinal lymph nodes were removed, and a single‐cell suspension was prepared. The cells were analyzed flow cytometrically, using monoclonal anti‐protein kinase C antibody. It was found that the number of lymph node cells in which protein kinase C was detected on the cell surface was significantly increased over that in non‐treated mice (p<0.01). On the basis of our results and discussions in the literature, it is thought that protein kinase C is related to the initiation of the contact hypersensitivity reaction.
Establishment of a Human Dermatofibrosarcoma Protuberans Cell Line: Cytological Characteristics
Authors: Shindo Yasuko , Akiyama Junichi , Yamaji Kazuhiko , Ishihara Yoko , Saida Toshiaki , Takase Yoshio 
Published: April 2015

AbstractWe established a dermatofibrosarcoma protuberans (DFSP) cell line derived from a DFSP tumor from a 69‐year‐old female. The population doubling time of the cells was about 45 hours. The cells were mostly round, although a few triangular or spindle‐shaped cells were also found. They could not be transplanted into nude mice.These cells had some histiocyte characteristics which are not observed in fibroblasts: 1) Electron microscopically, there were more lysosomes and phagolysosomes than in fibroblasts. Dense bodies and myelin figures derived from lysosomes were also observed. 2) Staining with acid phosphatase (ACPase), esterase, and α‐1‐antitrypsin was positive. 3) The cells had C3 receptors but no Fc receptors. However, there were two differences between DFSP cells in the established line and those in the early stages: in the former, the capacity for immunophagocytosis (Fc receptors and phagocytosis of Candida albicans) and collagen synthesis were almost entirely absent.
Lupus Erythematosus Profundus
Authors: Yamada Yoshitaka , Dekio Satoshi , Jidoi Joji , Ozasa Shozaburo 
Published: April 2015

AbstractA male adult patient with discoid lupus erythematosus (DLE) developed lupus erythematosus profundus. We decided to treat him with oral administration of dapsone, which proved to be very effective.
Congenital Alveolar Rhabdomyosarcoma Presenting as a Blueberry Muffin Baby
Authors: Kitagawa Nobuko , Arata Jirô , Ohtsuki Yuji , Hayashi Kazuhiko , Oomori Yasuhiro , Tomoda Takashi 
Published: April 2015

AbstractCongenital alveolar rhabdomyosarcoma in a newborn was reported. She had multiple subcutaneous tumors and looked like a blueberry muffin baby. From the clinical signs and the histological pictures, a diagnosis of neuroblastoma was considered. Laboratory data showed no abnormalities. Electron microscopic examination revealed myofilament and incipient stria formation.On the basis of these data, a final diagnosis of congenital alveolar rhabdomyosarcoma associated with multiple skin metastases was made.
A Case of Temporal Arteritis Successfully Treated with Recombinant Interleukin‐2
Authors: Kamide Ryoichi , Ishikawa Takeshi , Aizawa Hiroshi , Aoki Ikuko , Niimura Michihito 
Published: April 2015

AbstractAn 80‐year‐old Japanese woman with temporal arteritis was treated with systemic recombinant human interleukin‐2 (IL‐2) (1 × 106 unit/day for six weeks). The presenting symptoms of headache and skin necrosis and abnormal laboratory findings, such as an elevated erythrocyte sedimentation rate and CRP, promptly improved without any serious side effects. Although the pathogenesis of temporal arteritis and the mechanism(s) of the beneficial effect of IL‐2 on it still remain unknown, this preliminary study highly encourages further investigations.
Modulation of Bullous Pemphigoid Antigens by Gamma Interferon in Cultured Human Keratinocytes
Authors: Su Hong , Reano Alain , Hesse Sylvie , Viac Jacqueline , Thivolet Jean 
Published: April 2015

AbstractWe report the effects of human recombinant gamma interferon (γ‐IFN) on the expression of bullous pemphigoid (BP) antigens by human cultured keratinocytes.Secondary epidermal cell cultures were grown on 3T3 mouse fibroblasts; when confluent, some cultures were maintained in control medium while others were exposed to various concentrations of γ‐IFN (100, 200, 400 U/ml) for 14 days. The expression of BP antigens was analyzed by indirect immunofluorescence on epithelial sheets and immunoblotting of Tris, SDS, β‐mercaptoethanol culture extracts using different BP sera.Our results show that γ‐IFN alters the expression of BP antigens in a way varying according to the skin donor: we observed results ranging from complete loss and decreased expression to unchanged reactivity patterns. Thus, γ‐IFN modifies BP antigen expression; this behavior has been previously shown for other adhesion molecules such as fibronectin and thrombospondin. However, the observed variability of the expression of BP antigens according to the skin donor suggests an unexpected variability in keratinocyte sensitivity to gamma interferon, which remains to be explored both in vitro and in vivo.
Human Squamous Cell Carcinoma from Skin: Establishment and Characterization of a New Cell Line (HSC‐5)
Authors: Hozumi Yutaka , Kondo Shigeo , Shimoura Takako , Aso Kazuo 
Published: April 2015

AbstractA new cell line, designated as HSC‐5 and derived from human skin squamous cell carcinoma (SCC), has been established in vitro and maintained proliferative in continuous tissue culture for over two years. The cells grow in a monolayer in vitro and have anaplastic epithelioid features. The doubling time was about 35hr at the 30th passage. Chromosome analysis showed hypotetraploidy with a modal number of 76. A trial of transplantation of the cultured cells into nude mice was not successful. Analysis of cytokeratins from HSC‐5 by two‐dimensional gel electrophoresis revealed polypeptides No. 5, 8, 13, 18 and 19.The cell line is available to other investigators.
Chromosome Abnormalities in Skin Fibroblasts Probably Induced by an Anti‐cancer Drug
Authors: Sadamori Naoki , Honda Takeo , Toriyama Fumi 
Published: April 2015

AbstractChromosome findings in cultured skin fibroblasts from a patient treated with an anti‐cancer drug, pepleomycin sulfate, for his penis cancer are reported. In two batches of specimens (days 16 and 34, respectively), out of 120 cells examined, a total of 26 abnormal cells (21.7%) were found with no common chromosome abnormalities; there were no clones. This cytogenetic pattern of abnormalities without clones in cultured skin fibroblasts differs from that seen in congenital disease or radiation‐exposed skin fibroblasts. It is suggested that the anti‐cancer agent is the most likely etiological factor for these uncloned chromosome abnormalities in cultured skin fibroblasts.
Tiopronin‐induced Lichenoid Eruption in a Patient with Liver Disease and Positive Patch Test Reaction to Drugs with Sulfhydryl Group
Authors: Kurumaji Yuko , Miyazaki Kazuhiro 
Published: April 2015

AbstractA 62‐year‐old woman suffering from liver cirrhosis developed lichenoid eruptions after 2 years of treatment with tiopronin. The lesions healed spontaneously within a month after withdrawal of the drug. In patch testing, the patient reacted positively, not only to tiopronin, but also to captopril and D‐penicillamine, neither of which she had ever taken before. The provocation test was positive only to tiopronin, and its histological findings revealed lichenoid reaction. It is suggested that the sulfhydryl group may play a role in the etiology of tiopronin‐induced lichenoid drug eruption.
Granulation Tissue that Developed after a Minor Trauma in a Psoriatic Patient on Long‐term Etretinate Therapy
Authors: Katayama Hiroshi , Okabe Naomi , Kano Toshiyuki , Yaoita Hideo 
Published: April 2015

AbstractSynthetic analogues of retinoic acid, isotretinoin in particular, are known to cause granulation tissue in the nail sulcus and healing cystic acne. We observed granulation tissue that developed in a psoriatic patient on long‐term oral etretinate therapy (30 mg/day for 3 years), another synthetic analogue of retinoic acid. In August of 1988, the patient, a 44‐year‐old male, hit his lower leg against a wooden stick, resulting in a laceration. At that time he had relatively limited patches of psoriasis, but his skin was generally thin with teleangiectasia, even in areas without psoriasis, probably due to the long‐term use of oral etretinate and topical steroid. In spite of treatment with topical disinfectants and oral antibiotics, the wound progressed into granulation tissue surrounded by erythema and multiple punctate erosions with marked secretion of a clear exudate. The histological findings of the granulation tissue included the presence of proliferating microvessels and a relatively dense cell infiltration with a predominance of plasma cells on the sparse edematous connective tissue. Based on these findings, the rash was considered to be retinoid‐induced granulation tissue. As compared with previous reports, our case had the following characteristics: development of rash after minor trauma, rash on the lower leg, eosinophil infiltration into the rash.We believe that long‐term users of oral etretinate should be cautioned about the potential for delayed wound healing and the formation of granulation tissue.
Reproduction of the Skin Lesions of Polymorphous Light Eruption: A Case Report and a Review of the Japanese Literature
Authors: Kawada Akira , Hiruma Masataro , Noda Toshiaki , Kukita Atsushi 
Published: April 2015

AbstractA case of polymorphous light eruption with plaque‐type lesions is reported. A 55‐year‐old male had pruritic erythemas and plaques on the head, the face, the nape, the back of the hands, and the left loin within days following sun exposure. The patient's minimal erythema dose (MED) (60 mJ/cm2) of sunlamp irradiation for ultraviolet (UV) B was normal. Three once‐daily exposures to 2 MED of UVB (Epstein's repeat phototest technique) produced pruritic papules with a similar microscopic appearance to that of the patient's skin lesion, indicating that the action spectrum causing the lesion seemed to be within the UVB waveband.
Production of Interleukin 1 Alpha by a Trichilemmoma Cell Line
Authors: Nishioka Kiyoshi , Funato Tadao , Sato Yuko , Ohta Yukinori , Eto Hikaru , Katayama Ichiro , Nishiyama Shigeo 
Published: April 2015

AbstractIn order to obtain a good source of human epidermal cell derived thymocyte activating factor (ETAF) for biological study, several cell lines of epidermal origin were cultured. One of the trichilemmoma cell lines, KTL‐1, was found to produce ETAF satisfactorily in its culture supernatant. The culture supernatant was collected and analyzed to determine the nature of the ETAF activity. The ETAF activity was completely inhibited by anti‐IL 1α antibody, but not by anti‐IL 1β antibody. Messenger RNA expression by KTL‐1 cells was analyzed by using cDNA of IL 1α as a probe. Messenger RNA was not detected in KTL‐1 cells by either dot blot analysis or northern blot analysis. However, it was expressed weakly in the cytoplasm by in situ hybridization technique. Messenger RNA expression was easily detected in KTL‐1 cells stimulated by lipopolysaccharide (LPS). Messenger RNA expression reached a peak at 3 hours after stimulation of LPS. A trichilemmoma cell line, KTL‐1, can be a good source of human ETAF for biological study.
The Origin and Fate of Merkel Cell Granules
Authors: Nindl Margit , Nakagawa Hidemi , Ishibashi Yasumasa 
Published: April 2015

AbstractIn order to elucidate the origin and fate of Merkel cell granules (MCGs), electron microscopic studies were carried out in fetal rat skin at day 20 of gestation.In addition to the ordinary processing of the tissue, we incubated a part of the tissue with a solution containing ionophore A23187 in order to capture the rarely observable exocytotic event.Based on our findings, a hypothetical model for the life cycle of Merkel cell granules is proposed as follows: I) Granule morphogenesis takes place in the rough endoplasmic reticulum and the GERL, from where immature granules are budding off, thereby exhibiting a bristle‐like coat. II) Maturation and storage of MCGs takes place in the cytoplasm. III) Following stimulation, MCGs are released. IV) After the exocytotic granule release, MCG membranes are retrieved in the form of coated pits.
Evaluation of Skin Test Reactions in Patients with Non‐immediate Type Drug Eruptions
Authors: Osawa Junko , Naito Shizuo , Aihara Michiko , Kitamura Kazuko , Ikezawa Zenro , Nakajima Hiroshi 
Published: April 2015

AbstractSkin test reactions were evaluated in 242 patients who appeared to develop delayed type drug eruptions from the clinical course. The patch testing was positive in 62 (31.5%) of 197 patients tested and the intradermal testing in 105 (89.7%) of 117 patients. The positive ratios of intradermal testing were higher in maculopapular (MP), erythema multiforme (EM), and erythrodermic (ED) types than in eczematous (Ecz) type drug eruptions, while those of patch testing were comparatively high in ED, Ecz type, and anticonvulsant‐induced drug eruptions. It is considered that the combination of patch testing and intradermal testing is useful for determination of causative drugs in delayed type drug eruptions.
The Effect of Topical Minoxidil on Hair Follicular Cycles of Rats
Authors: Mori Osamu , Uno Hideo 
Published: April 2015

AbstractTo explore an easily accessible and reproducible model for examining the effect of minoxidil on hair growth, we studied the effect of minoxidil on the natural hair cycles of rats from birth to 80 days of age. During the 1st and 2nd postnatal cycles, the hair follicles grew very rapidly and the size of anagen follicles were markedly enlarged. In the 3rd cycle (50 days to approximately 100 days of age), duration of the telogen phase lasted approximately 20 days. Topical minoxidil, 1%, 3%, or 5% solution, applied on the backs of the rats from 23 days (weaning) to 80 days, induced a remarkable shortening of the telogen phase in the 3rd cycle. Although the dose‐dependent response was very minimal, rats treated with 3% or 5% minoxidil showed similar effects in the 4th cycle. Minoxidil, however, did not induce prolongation of the anagen phase, but increased the rate of DNA synthesis in the anagen bulb during the 2nd and 3rd cycles. These results suggest that minoxidil specifically stimulates the secondary germ of the telogen follicles, resulting in rapid progression to anagen follicles.
Proliferative Potential of Murine Melanoma Cells Cultured in or on Collagen Gel
Authors: Furukawa Masayoshi , Kono Takeshi , Tanii Tsukasa , Ishii Masamitsu , Hamada Toshio , Shibata Tadayoshi 
Published: April 2015

AbstractAs the first step in developing an in vitro model of melanoma cells infiltrating the dermis, B16 murine melanoma cells were cultured on and in type I collagen gels. Under these conditions, the melanoma cell adopted an elongated or dendritic form. Cell proliferation was suppressed in the culture system using the collagen gel as compared with the conventional monolayer culture on plastic. Microcinematographically, this suppression was found to be due to an extension of the cell cycle time of each individual cell.On the other hand, there were no appreciable differences in proliferation pattern between the cells cultured on type I and IV collagen film and those cultured on plastic.These results suggest that there are interactions between type I collagen in the gel form and melanoma cells, especially with respect to cell growth.
A Case of Neurofibrosarcoma Associated with Neurofibromatosis: Light Microscopic, Ultrastructural, Immunohistochemical and Biochemical Investigations
Authors: Morioka Naoko , Tsuchida Tetsuya , Etoh Takafumi , Ishibashi Yasumasa , Otsuka Fujio 
Published: April 2015

AbstractA case of neurofibrosarcoma (NFS) with neurofibromatosis was studied by light microscopic, ultrastructural, immunohistochemical and biochemical methods. Histologically, spindle‐shaped tumor cells with atypical hyperchromatic nuclei were arranged in a fascicular or sheet‐like fashion. Electron microscopic examination revealed discontinuous basement membrane‐like structures. Immunohistochemical study revealed S100 protein α chains in tumor cells.A biochemical analysis showed large amounts of gangliosides (sialic acid 13.5 μg/g wet tissue weight) in tumors. GM3 and GD3 were the major components; these results were compatible with the NFS ganglioside patterns found in our previous study.Results confirmed the diagnosis of NFS and indicate that NFS clearly has some neuroectodermal characteristics and that Schwann cells may possibly be related to its origin.
Two Cases of Pilomatrix Carcinoma
Authors: Miyahara Hiroko , Imayama Shuhei , Hashizume Tamiko , Takeishi Masaaki , Hori Yoshiaki , Kohda Hiromu 
Published: April 2015

AbstractWe report two cases of pilomatrix carcinoma. Wide surgical excision, radiation, and chemotherapy were required for the first patient. The tumor eventually revealed the characteristics of a carcinoma, both clinically and histopathologically, as it recurred. In the second case, although the tumor exhibited the histology of pilomatricoma in the central part of the lesion, there were numerous outgrowing irregular nests of basaloid tumor cells with cellular atypia and frequent mitoses; these are the characteristic features of the pilomatrix carcinoma in its early stages.
Distribution of Glial Fibrillary Acidic Protein (GFAP) in the Intermediate Filaments of the Cultured Cells from a Patient with Tuberous Sclerosis
Authors: Onodera Kazukiyo , Takahashi Tetsuo , Watanabe Ryoji , Ishibashi Yasumasa , Sasaki Masafumi , Kimura Genki 
Published: April 2015

AbstractWe established a cell line (TS) from adenoma sebaceum of a patient with tuberous sclerosis. Through our previous studies, the abnormal cell division and dysfunction of TS cells were indicated. Glial fibrillary acidic protein (GFAP) and 55kd protein had been found to be major cytoskeletal proteins in these cells. This time we have examined the structure and distribution of cytoskeletons in TS cells with immunoelectron microscopy. TS cells were found to coexpress GFAP and vimentin‐like substance; both structures seem to be closely related. The coexistence of a few kinds of proteins integrated in the cytoskeletons might lead to the abnormal behavior of the nucleus during the process of cell division.