bluuebunny/crossref_metadata_2025_split huggingface dataset:
Permeability of the landscape matrix between amphibian breeding sites
Authors: Van Buskirk Josh 
Published: November 2012

AbstractFor organisms that reproduce in discrete habitat patches, land cover between patches (known as the matrix) is important for dispersal among breeding sites. Models of patchy populations often incorporate information on the permeability of the matrix to dispersal, sometimes based on expert opinion. I estimated the relative resistance to gene flow of land cover types and barriers usingFSTcalculated from microsatellite markers in two amphibians, within an 800‐km2area in northernSwitzerland. The species included a frog (Rana temporaria: 996 individuals, 48 populations, seven markers) and a newt (Triturus alpestris: 816 individuals, 41 populations, seven markers). Open fields and urban areas were more resistant to gene flow than forested land; roads and highways also reduced permeability. Results were similar for the two species. However, differences in resistance among matrix elements were relatively low: gene flow through urban areas was reduced by only 24–42% relative to forest; a divided highway reduced gene flow by 11–40% and was 7–8 times more resistant than a secondary road. These data offer an empirically based alternative to expert opinion for setting relative resistance values in landscape models.
Two‐Step Spin Transition in an Iron(II) Coordination Network Based on Flexible Bitopic Ligand 1‐(Tetrazol‐1‐yl)‐3‐(1,2,3‐triazol‐1‐yl)propane
Authors: Białońska Agata , Bronisz Robert , Kusz Joachim , Zubko Maciej 
Published: November 2012

AbstractFlexible bitopic ligand 1‐(tetrazol‐1‐yl)‐3‐(1,2,3‐triazol‐1‐yl)propane (ptrtz) reacts with Fe(ClO4)2·6H2O to form two‐dimensional (2D) coordination network [Fe(ptrtz)3](ClO4)2·MeCN (1) in which two chemically different spin crossover (SCO) FeII centers are observed. Although two 1,2,3‐triazole and four tetrazole rings are in the first coordination sphere of the Fe1 ions, Fe2 ions are coordinated to four 1,2,3‐triazole and two tetrazole rings. Symmetry‐related FeII ions are bridged by two disordered ptrtz molecules resulting in the formation of chains that are extended in the same direction. Alternately arranged chains containing Fe1 or Fe2 ions are cross‐linked by single ptrtz molecules to form 2D layers. At room temperature, the complex remains in a high‐spin (HS) form (χMT = 3.46 cm3 K mol–1). Spin crossover in 1 occurs in two steps separated by an inflection point at 140 K (χMT = 2.68 cm3 K mol–1, γHS ≈ 0.76). The first step is gradual and between 220 and 140 K about a quarter of all the iron(II) ions change their spin state (T1↓ = T1↑ = 165 K). Single‐crystal X‐ray diffraction studies revealed that upon lowering the temperature from 220 to 140 K, spin crossover occurs for only half of the iron(II) ions occupying Fe2 sites. It is accompanied by an alteration of the occupancy factor of ptrtz molecules bridging the Fe2 ions. Iron(II) ions occupying Fe1 sites remain in the HS form, which inhibits compression of the polymeric layer in the Fe1···Fe1 and Fe2···Fe2 linking direction. Upon further lowering the temperature, iron(II) ions occupying Fe1 sites and the rest of the iron(II) ions occupying Fe2 sites change their spin state. The second step of the spin transition is very abrupt and is accompanied by a hysteresis loop (T2↓ = 90 K, T2↑ = 96 K). The change in the spin state by iron(II) ions occupying Fe1 and Fe2 sites involves, contrary to first step, a significant shortening of the Fe1···Fe1 and Fe2···Fe2 separations.
Synthesis, Characterization, and Selected Properties of 7‐ and 12‐Ammoniocarba‐closo‐dodecaboranes
Authors: Konieczka Szymon Z. , Himmelspach Alexander , Hailmann Michael , Finze Maik 
Published: November 2012

AbstractThe inner salts 12‐H3N‐closo‐1‐CB11H11 (H1) and 7‐H3N‐12‐Hal‐closo‐1‐CB11H10 [Hal = F (H3), Cl (H4)] were synthesized by a microwave‐assisted Buchwald–Hartwig amination starting from the corresponding monoiodinated {closo‐1‐CB11} clusters and LiN(SiMe3)2. H1 was methylated to result in the zwitterion 12‐Me3N‐closo‐1‐CB11H11 (2). All new compounds were characterized by multinuclear NMR spectroscopy, elemental analysis, and mass spectrometry. The crystal structures of H1·Me2CO and 2 were determined. The pKa value of H1 in aqueous solution was estimated by potentiometric titration and by 11B{1H} NMR spectroscopic studies. The stepwise deprotonation of H1 with nBuLi in [D8]THF was studied by multinuclear NMR spectroscopy. In a first step, the amine 1 is obtained and then the amide [12‐HN‐closo‐1‐CB11H11]2– and finally the trianion [12‐HN‐closo‐1‐CB11H10]3– or more probably lithiated species thereof are formed. The experimental spectroscopic and structural data, as well as the pKa value of H1 are compared to values derived from DFT and ab initio calculations.
Synthesis of Isobavachalcone and Some Organometallic Derivatives
Authors: Grealis John P. , Müller‐Bunz Helge , Ortin Yannick , Casey Michael , McGlinchey Michael J. 
Published: November 2012

AbstractIsobavachalcone [2′,4,4′‐trihydroxy‐3′‐(3″‐methyl‐2″‐butenyl)chalcone, 1] is a prenylated chalcone that has broad biological activity, in particular against neuroblastomas, the most common cancer in infancy. It is currently commercially available at a cost of $190/mg by extraction from Psoralea corylifolia and a number of other African and Asian plants. Several synthetic routes have been explored, and the most efficient procedure involves the palladium‐catalysed Stille coupling of 3‐iodo‐2,4‐bis(methoxymethoxy)acetophenone (25) with prenyltributyltin, Claisen–Schmidt condensation with 4‐(methoxymethoxy)benzaldehyde to form the triply MOM‐protected prenylchalcone 27 and finally deprotection with 2 M HCl in methanol to form isobavachalcone in an overall yield of 15 % over five steps. The X‐ray crystal structures of 2,4‐dihydroxy‐3‐iodoacetophenone (21) and of several prenylated chalcones are reported, including the elucidation of their hydrogen‐bonding networks in the solid state. The synthetic route has been extended to include organometallic derivatives in which the 4‐(methoxymethoxy)benzaldehyde used in the Claisen–Schmidt condensation has been replaced by formylferrocene, formylruthenocene or (η5‐formylcyclopentadienyl)(η4‐tetraphenylcyclobutadiene)cobalt to form the corresponding analogues of isobavachalcone containing organometallic sandwich moieties.
Differential Recognition of Mannose‐Based Polysaccharides by Tripodal Receptors Based on a Triethylbenzene Scaffold Substituted with Trihydroxybenzoyl Moieties
Authors: Carrero Paula , Ardá Ana , Alvarez Mónica , Doyagüez Elisa G. , Rivero‐Buceta Eva , Quesada Ernesto , Prieto Alicia , Solís Dolores , Camarasa María José , Peréz‐Pérez María Jesús , Jiménez‐Barbero Jesús , San‐Félix Ana 
Published: November 2012

AbstractThe tripodal receptors 1 and 2 based on a triethylbenzene scaffold substituted with trihydroxybenzoyl groups have been synthesised. The conformational preferences and carbohydrate‐binding ability of 1 and 2 have been examined by NMR spectroscopy and modelling procedures. The results reveal that the particular structural pre‐organisation of 2 facilitates the recognition, in a highly competitive medium (DMSO), of a mannose‐based polysaccharide consisting of a linear saccharide chain continuously decorated by α(1→2)‐linked branching mannose moieties. By contrast, other α(1→2)‐substituted polysaccharides or different monosaccharides are not bound, revealing the selectivity of the interaction. Due to the importance of α(1→2) mannosides, which are abundantly present on the glycan shield of several pathogens, the results reported here open attractive prospects for the potential application of 2 or its derivatives in future antiinfective strategies.
Haplotypes in the 5′‐untranslated region of the CYP1A2gene are inversely associated with lung cancer risk but do not correlate with caffeine metabolism
Authors: Gervasini Guillermo , Ghotbi Roza , Aklillu Eleni , San Jose Carmen , Cabanillas Agustin , Kishikawa Junko , Benitez Julio , Carrillo Juan Antonio 
Published: November 2012

AbstractIn this study, we analyzed the influence of CYP1A2 genetic variation and enzyme activity on lung cancer risk in a high‐incidence area. A total of 95 lung cancer patients and 196 controls were genotyped for the −3860G/A, −3113A/G, −2467T/delT, −739T/G, and −163C/A polymorphisms in the 5′‐untranslated region of the gene. In addition, a subset of 70 patients and 115 controls were phenotyped by high‐performance liquid chromatography determination of the caffeine metabolic ratio (CMR). The −2467T/delT polymorphism and the CYP1A2*1V haplotype (‐163C>A, −2467T>delT) were inversely associated with lung cancer risk (odds ratio [OR] = 0.47 [0.2–0.9]; P = 0.02 and OR = 0.13 [0.02‐1.0]; P = 0.04; respectively). In addition, the CYP*1A/*1V and *1F (‐163C>A)/*1D (−163C>A, −2467T>delT) diplotypes were absent in the patients group, whereas accounting for 7.1% (P = 0.017) and 5.6% (P = 0.037) of controls, respectively. Mean CMR was significantly higher in patients than in controls (10.50 ± 17.31 vs. 6.52 ± 6.26, P = 0.01) but regression analyses did not yield significant ORs for the association with lung cancer risk. Similarly, no significant correlations were found between any genetic variant and enzyme activity. Several CYP1A2 haplotypes and diplotypes containing the −2467delT variant were associated with lower lung cancer risk; however, they did not correlate with significant changes in CYP1A2 metabolic activity toward caffeine. Environ. Mol. Mutagen., 2013. © 2012 Wiley Periodicals, Inc.
Socio‐demographic and clinical profile of chronic pain with neuropathic characteristics in sub‐Saharan African elderly
Authors: Lekpa F.K. , Ndongo S. , Ka O. , Zeba D. , Compaoré C. , Pouye A. , Ka M.M. , Diop T.M. 
Published: November 2012

AbstractData on characteristics of neuropathic pain (NP) in sub‐Saharan Africa are scarce, especially in the elderly. We conducted this study to appreciate the socio‐demographic and clinical profile of chronic pain (CP) with neuropathic characteristics in sub‐Saharan African elderly with musculoskeletal pain. From January to December 2011, we performed a cross‐sectional study in all Rheumatology outpatients over 60 years at the Center for Gerontology and Geriatrics, Dakar, Senegal. In this study, we included patients who experienced musculoskeletal pain for 3 months or longer (CP) and with a DN4 score ≥ 4 (NP). A complete clinical examination was performed to make the diagnosis of NP ‘definite’ or ‘probable’, and to identify the aetiologies of NP. During the study period, 698 outpatients were examined. There were 394 out of the 549 patients over 60 years who reported CP. Among them, 28 patients (7.1%) scored ≥4 on the DN4 questionnaire. Female patients, low educational attainment, manual professions, non‐workers and diabetes were associated with NP (p < 0.05). The symptoms described by patients with NP, often intricate, were lumboradiculalgia (n = 9), cervico‐brachial neuralgia (n = 3), polyneuropathy (n = 12) and mononeuropathy (n = 6). The presumed aetiologies in patients with NP were: chronic spine diseases (n = 14), painful diabetic peripheral neuropathy (n = 8), Sjögren's syndrome (n = 1), tarsal tunnel syndrome in rheumatoid arthritis (n = 1) and bone metastasis (n = 1). No aetiology was identified among three patients. Chronic spine diseases associated with radiculopathies and diabetic neuropathy are the main causes of NP, well detected by DN4 questionnaire and clinical examination in Senegalese sub‐Saharan African elderly.
Guided Internet‐delivered cognitive behavioural therapy for chronic pain patients who have residual symptoms after rehabilitation treatment: Randomized controlled trial
Authors: Buhrman M. , Fredriksson A. , Edström G. , Shafiei D. , Tärnqvist C. , Ljótsson B. , Hursti T. , Gordh T. , Andersson G. 
Published: November 2012

AbstractBackgroundChronic pain can be treated with cognitive behavioural therapy delivered in multidisciplinary settings. However, relapse is likely, and there is a need for cost‐effective secondary interventions for persons with residual problems after rehabilitation. The aim of the present study was to investigate the effects of a guided Internet‐delivered cognitive behavioural intervention for patients who had completed multidisciplinary treatment at a pain management unit.MethodsA total of 72 persons with residual pain problems were included in the study and were randomized to either treatment for 8 weeks or to a control group who were invited to participate in a moderated online discussion forum. The participants had different chronic pain conditions, and a majority were women (72%). Twenty‐two percent of the participants dropped out of the study before the post‐treatment assessment.ResultsIntent‐to‐treat analyses demonstrated differences on the catastrophizing subscale of the Coping Strategies Questionnaire (Cohen's d = 0.70), in favour of the treatment group but a small within‐group effect. Differences were also found on other measures of pain‐related distress, anxiety and depressive symptoms. A 6‐month follow‐up exhibited maintenance of improvements.ConclusionsWe conclude that Internet‐delivered treatment can be partly effective for persons with residual problems after completed pain rehabilitation.
Low back pain‐related beliefs and likely practice behaviours among final‐year cross‐discipline health students
Authors: Briggs A.M. , Slater H. , Smith A.J. , Parkin‐Smith G.F. , Watkins K. , Chua J. 
Published: November 2012

AbstractBackgroundEvidence points to clinicians' beliefs and practice behaviours related to low back pain (LBP), which are discordant with contemporary evidence. While interventions to align beliefs and behaviours with evidence among clinicians have demonstrated effectiveness, a more sustainable and cost‐effective approach to positively developing workforce capacity in this area may be to target the emerging workforce. The aim of this study was to investigate beliefs and clinical recommendations for LBP, and their alignment to evidence, in Australian university allied health and medical students.MethodsFinal‐year students in chiropractic, medicine, occupational therapy, pharmacy and physiotherapy disciplines in three Western Australian universities responded to a survey. Demographic data, LBP‐related beliefs data [modified Health Care Providers Pain and Impact Relationship Scale (HC‐PAIRS) and the Back Pain Beliefs Questionnaire (BBQ) ] and activity, rest and work clinical recommendations for an acute LBP clinical vignette were collected.ResultsSix hundred two students completed the survey (response rate 74.6%). Cross‐discipline differences in beliefs and clinical recommendations were observed (p > 0.001). Physiotherapy and chiropractic students reported significantly more helpful beliefs compared with the other disciplines, while pharmacy students reported the least helpful beliefs. A greater proportion of chiropractic and physiotherapy students reported guideline‐consistent recommendations compared with other disciplines. HC‐PAIRS and BBQ scores were strongly associated with clinical recommendations, independent to the discipline of study and prior experience of LBP.ConclusionsAligning cross‐discipline university curricula with current evidence may provide an opportunity to facilitate translation of this evidence into practice with a focus on a consistent, cross‐discipline approach to LBP management.
Genetic and environmental influences on non‐specific neck pain in early adolescence: A classical twin study
Authors: Ståhl M.K. , El‐Metwally A.A. , Mikkelsson M.K. , Salminen J.J. , Pulkkinen L.R. , Rose R.J. , Kaprio J.A. 
Published: November 2012

AbstractBackgroundPrevalence of neck pain has increased among adolescents. The origins of adult chronic neck pain may lie in late childhood, but for early prevention, more information is needed about its aetiology. We investigated the relative roles of genetic and environmental factors in early adolescent neck pain with a classic twin study.MethodsFrequency of neck pain was assessed with a validated pain questionnaire in a population‐based sample of nearly 1800 pairs of 11–12‐year‐old Finnish twins. Twin pair similarity for neck pain was quantified by polychoric correlations, and variance components were estimated with biometric structural equation modelling.ResultsPrevalence of neck pain reported at least once monthly was 38% and at least once weekly 16%, with no significant differences between gender and zygosity. A greater polychoric correlation in liability to neck pain was found in monozygotic (0.67) than for dizygotic pairs (0.38), suggesting strong genetic influences. Model fitting indicated that 68% (95% confidence interval 62–74) of the variation in liability to neck pain could be attributed to genetic effects, with the remainder attributed to unshared environmental effects. No evidence for sex‐specific genetic effects or for sex differences in the magnitude of genetic effects was found.ConclusionsGenetic and unique environmental factors seem to play the most important roles in liability to neck pain in early adolescence. Future research should be directed to identifying pathways for genetic influences on neck pain and in exploring effectiveness of interventions that target already identified environmental risk factors.
In vitro osteoblast response to ferritic stainless steel fiber networks for magneto‐active layers on implants
Authors: Malheiro V. N. , Skepper J. N. , Brooks R. A. , Markaki A. E. 
Published: November 2012

AbstractThe use of a porous coating on prosthetic components to encourage bone ingrowth is an important way of improving uncemented implant fixation. Enhanced fixation may be achieved by the use of porous magneto‐active layers on the surface of prosthetic implants, which would deform elastically on application of a magnetic field, generating internal stresses within the in‐growing bone. This approach requires a ferromagnetic material able to support osteoblast attachment, proliferation, differentiation, and mineralization. In this study, the human osteoblast responses to ferromagnetic 444 stainless steel networks were considered alongside those to nonmagnetic 316L (medical grade) stainless steel networks. While both networks had similar porosities, 444 networks were made from coarser fibers, resulting in larger inter‐fiber spaces. The networks were analyzed for cell morphology, distribution, proliferation, and differentiation, extracellular matrix production and the formation of mineralized nodules. Cell culture was performed in both the presence of osteogenic supplements, to encourage cell differentiation, and in their absence. It was found that fiber size affected osteoblast morphology, cytoskeleton organization and proliferation at the early stages of culture. The larger inter‐fiber spaces in the 444 networks resulted in better spatial distribution of the extracellular matrix. The addition of osteogenic supplements enhanced cell differentiation and reduced cell proliferation thereby preventing the differences in proliferation observed in the absence of osteogenic supplements. The results demonstrated that 444 networks elicited favorable responses from human osteoblasts, and thus show potential for use as magnetically active porous coatings for advanced bone implant applications. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Comparison of the Bactec Fx Plus, Mycosis IC/F, Mycosis/F Lytic Blood Culture Media and the BacT/Alert 3D FA Media for Detection of Candida species in Seeded Blood Culture Specimens Containing Therapeutic Peak Levels of Fluconazole
Authors: Jekarl Dong Wook , Lee So‐Young , Lee Seungok , Park Yeon‐Joon , Lee Jehoon , Baek Sun Mi , An Yeon Ju , Ock Sun Myeong , Lee Mi‐Kyung 
Published: November 2012

BackgroundThe performance of Bactec Fx Plus Aerobic/F (PA), Mycosis IC/F (MF), Myco/F Lytic (ML) media and BacT/Alert 3D FA (FA) media in detecting 15 Candida isolates in blood cultures to which fluconazole had been added was investigated.MethodsPA with resin, MF, ML media (n = 360), and FAmedia (n = 120) were tested against Candida albicans, C. tropicalis, C. parapsilosis, and C. krusei. As the peak plasma concentration after single oral doses of fluconazole 100, 200, and 400 mg was equivalent to peak level of 1.9, 4.7, and 6.7 mg/l, respectively, corresponding fluconazole was added. Time to detection (TTD) was measured.ResultsOverall TTD (mean hour ± standard deviation) for PA, FA, MF, and ML was as follows: 24.5 ± 7.3, 27.0 ± 7.5, 31.9 ± 21.3, and 37.7 ± 30.1, respectively. TTD of PA was shorter compared to other media. The effect of fluconazole was limited in PA and FA, but MF and ML showed delayed TTD. Larger inoculum size showed shorter TTDin PA and FA.ConclusionTTD of Bactec Fx Plus Aerobic/F was more than 2.5 hr faster among the tested media. As thus system and media are unaffected by added fluconazole, it could be used for the diagnosis of candidemia in the clinical settings including the patients who have been treated empirically with fluconazole at the time when blood cultures were drawn.
Is Identification of Anti‐SSA and/or ‐SSB Antibodies Necessary in Serum Samples Referred for Antinuclear Antibodies Testing?
Authors: Yang Zaixing , Liang Yan , Zhong Renqian 
Published: November 2012

BackgroudAnti‐SSA and ‐SSB antibodies are clinically important antinuclear antibodies in patients with systemic rheumatic diseases.MethodsWe evaluated fluorescence characteristics and clinical significance of anti‐SSA and ‐SSB and explored whether identification of these antibodies was necessary in clinical application.ResultsOf 4,978 consecutive samples, 259 showed anti‐SSA or ‐SSB reactivity, clinical information of which were analyzed. Compared with SSA+SSB− and SSA−SSB+ group, SSA+SSB+ group showed a lower proportion (0.5%) of negative specimens detected with antinuclear antibody assay and a higher proportion (53.5%) of specimens with high titer. Anti‐SSA‐ and/or ‐SSB‐positive samples presented various patterns. However, 64.6% of SSA+SSB+ samples presented speckled pattern, significantly higher than SSA+SSB− and SSA−SSB+ samples. A total of 475 specimens containing anti‐SSA or ‐SSB were obtained from 302 individuals. Clinical information was obtained for 259 of them, which were further analyzed. The prevalence of Sjögren syndrome (SS) was significantly higher, and that of other diseases was lower in SSA+SSB+ than in SSA+SSB− group. Of the 259 individuals, 71 anti‐SSA‐ and/or ‐SSB‐positive patients had blood drawn on 2 or more occasions over the 2‐year study period. The number of tests per patient was 2.7 ± 1.1. In 7 of the 71 patients, anti‐SSA was observed in some but not all samples, so was anti‐SSB in 6.ConclusionIn conclusion, the identification of anti‐SSA and ‐SSB antibodies is necessary, when corresponding diseases are suspected. The specificity of anti‐SSA for the diagnosis of SS could be improved, when combined with anti‐SSB. SS patients need not be identified frequently for anti‐SSA and ‐SSB because of their stability.