bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feeding milk replacer instead of whole milk affects blood plasma proteome and lipid profile in preruminant calves
Authors: Lepczyński A. , Herosimczyk A. , Ożgo M. , Skrzypczak W.F. 
Published: May 2015

Abstract
The study was undertaken to determine the effect of feeding milk or milk-replacer on the blood plasma proteome and lipid profile in calves during the second week of life. Feeding milk-replacer significantly decreased the expression of plasma apoA-I. Age of calves affected apoA-I expression, which was higher on the 8th than on the 11th and 14th day of life. A significant effect of interaction between diet and age was also observed. The expression of apoA-IV, was significantly affected by diet and was lower in calves fed milk replacer. Expression of this protein was significantly lower at the 8th day of life and was up-regulated in the calves fed milk-replacer at the second week of life. Calves fed milk-replacer had greater expression of haptoglobin, which differed significantly between days of blood sampling, being higher on the 8th than on the 11th and 14th day. The interactive effect of diet and age affected haptoglobin expression, which was successively down-regulated in calves fed milk replacer. Diet had a significant effect on the plasma lipid profile. Animals fed milk had a greater concentration of TC, HDLC and LDLC. The composition of milk-replacer, especially fat source, is probably the main factor that affects expression of proteins involved in cholesterol metabolism and level of components of lipid profile in calves fed formula. We claim that the initially increased level of haptoglobin, followed by its decrease during the second week of life in calves fed milk-replacer may indicate the presence of short-term stress induced by changes in the feeding system.
Poultry flocks as a source of Campylobacter contamination of broiler carcasses
Authors: Wieczorek K. , Osek J. 
Published: May 2015

AbstractCampylobacterinfection is the leading foodborne bacterial gastroenteritis worldwide and the bacteria are frequently isolated from the intestines of chickens. The broiler meat contamination withC. jejuniorC. colimay occur during slaughter processing. The aim of the study was to investigate the prevalence ofCampylobacterin poultry flocks and the corresponding broiler carcasses in 15 districts (voivodeships) all over Poland. A total of 128 samples from broiler flocks and the corresponding carcasses were collected between February 2011 and April 2013. TheCampylobacterisolation and species identification were performed according to ISO 10272-1 standard and with PCR. It was found that 112 flock (96.5%) were contaminated with campylobacters, eitherC. jejuni(77 samples; 68.7%) orC. coli(35 flocks; 31.3%). Analysis of the corresponding chicken carcasses tested after chilling revealed that 77 out of 128 (60.2%) samples were positive forCampylobacter, eitherC. jejuni(58; 75.3%) orC. coli(19; 24.7%). Most of the carcasses were contaminated with the sameCampylobacterspecies as identified in the corresponding flock before slaughter. As tested by PCR, out of the 77 crops withC. jejuni58 were positive for the same bacterial species. On the other hand, out of the remaining 35 flocks infected withC. coli, only 19 corresponding carcass samples were contaminated withC. coli. In three cases in the slaughtered flocksC. jejuniwas identified but in the same carcassesC. coliwas found. The opposite findings (flock positive forC. colibut the corresponding carcasses contaminated withC. jejuni) were seen in six voivodeships. It was also observed that several carcass samples were negative forC. jejuniandC. colialthough the original flocks wereCampylobacter-positive before slaughter (total 36 of the 77 samples; 46.7%). On the other hand, some carcasses were contaminated withCampylobacteralthough the flocks were negative for these bacteria (9 samples; 11.7%) which may also be due to internal contamination during slaughter of broilers.
Equid herpesvirus type 1 (EHV-1) disrupts actin cytoskeleton during productive infection in equine leukocytes
Authors: Drebert Z. , Golke A. , Cymerys J. , Słońska A. , Chmielewska A. , Tucholska A. , Bańbura M.W. 
Published: May 2015

Abstract
Equid herpesvirus type 1 (EHV-1) is a prevalent causative agent of equine diseases worldwide. After primary replication in the respiratory epithelium the virus disseminates systemically through a peripheral blood mononuclear cell (PBMC)-associated viraemia. EHV-1 is the only alphaherpes-virus known so far which is capable of establishing latent infection not only in neurons but also in immune system cells (mainly in lymphocytes and macrophages). Since leukocytes are not the target cells for viral replication but are used to transport EHV-1 to the internal organs, the question remains how the virus avoids the immune response and whether it could potentially be associated with virus-induced cytoskeletal rearrangements. Therefore, the aim of this study was to investigate the progress of EHV-1 replication in leukocytes stimulated by phytohemagglutinin and the impact of EHV-1 infection on the actin cytoskeleton. Using the real-time PCR method we evaluated the quantity of viral DNA from samples collected at indicated time points post infection. In order to examine possible changes in actin cytoskeleton organization due to EHV-1 infection, we performed immunofluorescent staining using TRITC-phalloidin conjugate. The results showed that EHV-1 replicates in leukocytes at a restricted level but with the accompaniment of chromatin degradation. Simultaneously, infection with EHV-1 caused disruption of the actin cytoskeleton; this was particularly apparent in further stages of infection. Disruption of the actin cytoskeleton may lead to the limited release of the virus from the cells, but may be also beneficial for the virus, since at the same time it potentially impairs the immune function of leukocytes.
Pharmacokinetics of propofol in rainbow trout following bath exposure
Authors: Gomułka P. , Fornal E. , Berecka B. , Szmagara A. , Ziomek E. 
Published: May 2015

Abstract
Propofol, 2,6-diisopropylphenol, seems to be a good candidate as a fish anaesthetic, however, no study regarding propofol influence on fish has yet been reported. The aim of this study was to examine propofol pharmacokinetics in rainbow trout (Oncorhynchus mykiss) following bath exposure. Fish (n = 100) were exposed to an aqueous propofol bath at 12°C and 17°C; propofol concentration in the bath was 10 mg L−1. Plasma concentration-time profiles were determined using LC-MS, and pharmacokinetic parameters were calculated. Propofol was absorbed quickly at both temperatures. Its concentration reached 13.8±2.7 μg mL−1 and 16.1±2.1 μg mL−1 at 12°C and 17°C, respectively, during the first minute of exposure. Blood plasma propofol decreased rapidly to 6.8±0.7 μgm L−1 and 6.3±2.2 μg mL−1 at 12°C and 17°C respectively, during the first 10 minutes of the recovery. The half-life time of propofol was 1.5 h and 1.1 h at 12°C and 17°C, respectively. We found propofol anaesthesia in trout effective and safe. However, it caused a gradual decrease of respiratory rate, and therefore a specific anaesthesia protocol should be developed.
The Use of Some Morphological Traits for the Assessment of Genetic Diversity in Spinach (Spinacia Oleracea L.) Landraces
Authors: Ebadi-Segheloo Asghar , ali Asadi-Gharneh Hossein , Mohebodini Mehdi , Janmohammadi Mohsen , Nouraein Mojtaba , Sabaghnia Naser 
Published: May 2015

ABSTRACT
Investigation of native accessions of spinach (Spinacia oleracea L.) would be aid in the development of new genetically improved varieties, so in this research 121 spinach landraces, collected from the various spinach growing areas of Iran, were evaluated to determine their diversity using several agro-morphological traits. High coefficients of variation (CV) were recorded in fresh yield, leaf area and dry yield. Using principal component (PC) analysis, the first three PCs with eigenvalues more than 0.9 contributed 80.56% of the variability among accessions. The first PC was related to leaf yield performance (fresh and dry yields, leaf numbers at flowering and lateral branches) while the PC2 was related to leaf characteristic (leaf width, petiole length, petiole diameter and leaf area). The third PC was related to seed characteristic (seed yield and 1000-seed weight) and was named as seed property component. The 121 spinach landraces were grouped into six clusters using cluster analysis. Each cluster had some specific characteristics of its own and the clusters I and II were clearly separated from clusters III and V and also from clusters IV and VI. The studied accessions are an important resource for the generation of a core collection of spinach in the world. The results of present research will support tasks of conservation and utilization of landraces in spinach breeding programs.
Evaluation of the Use of Spring Rapeseed in Phytoremediation of Soils Contaminated with Trace Elements and Their Effect on Yield Parameters
Authors: Szulc Piotr Mirosław , Kobierski Mirosław , Majtkowski Włodzimierz 
Published: May 2015

ABSTRACT
The experimental material was made up by the plant organs of Brassica napus L. from a pot experiment during one vegetation period. There was investigated the effect of relatively high concentration of zinc, copper, lead and cadmium in soil on the rapeseed yield, the content of protein and oil in seeds. The impact of metals was defined based on the content of selected fatty acids in oil extracted from seeds. The highest contents of zinc and copper were found in leaves, lead - in roots and cadmium - in stems. The biological concentration factor values were respectively calculated for all the rapeseed organs. For Cu and Pb the values of biological concentration factor were low and very low for all the plant organs. The doses of Zn (300 mg × kg-1, 600 mg × kg-1) and Cu (80 mg × kg-1, 160 mg × kg-1) applied in the pot experiment resulted in the translocation of metals from the roots to the leaves. The doses of lead (400 mg × kg-1, 1600 mg × kg-1) did not trigger any translocation of that metal from the roots to the above-ground rapeseed plant parts, however, after the application of the cadmium doses (2 mg × kg-1, 6 mg × kg-1), there was recorded a clear translocation of Cd to the rapeseed stems and the leaves. A relatively high content of zinc, copper, lead and cadmium in soil had a significant effect neither on the yield parameters and nor on the qualitative characters of the rapeseed seed. Neither did they affect the content of protein, fat and fatty acids in seed-extracted oil. The results of the pot experiment suggest that spring rapeseed is suitable for the phytoremediation of moderately heavy-metalcontaminated soils.
Modification of biaxially oriented polypropylene films using dicyclopentadiene based hydrogenated hydrocarbon resin
Authors: Kong Won-Suk , Ju Tae-Jun , Park Jun-Hyo , Lee Jae-Wook , Yoon Ho Gyu 
Published: May 2015

Abstract
The morphologies, crystalline structures, and thermal and rheological behaviors of isotactic polypropylene (iPP) blended with dicyclopentadiene based hydrogenated hydrocarbon resin (HCR) were investigated using scanning electron microscopy (SEM), wide-angle X-ray diffractometry, and differential scanning calorimetry in detail. Furthermore, the transparency, mechanical properties, and water vapor and oxygen permeability of biaxially oriented polypropylene films, fabricated from these iPP/HCR blends using a biaxial drawing machine, were examined. It was found that crystallinity varies with the HCR content of the iPP/HCR blend. In addition, amorphous HCR with its high glass transition temperature (T

g
) upon blending with iPP’s amorphous structure affords improvements in various film properties, such as mechanical properties, transparency, and gas permeabilities.
The Design of Ship Autopilot by Applying Observer - Based Feedback Linearization
Authors: Zwierzewicz Zenon 
Published: May 2015

Abstract
The paper considers the problem of ship autopilot design based on Bech’s model of the vessel. Since the model is highly nonlinear and some of the state vector coordinates are unavailable, the control system synthesis is performed by means of an output feedback linearization method combined with a nonlinear observer. The asymptotic stability of the overall system has been proven, including the asymptotic stability of the system internal dynamics. The performed simulations of the ship course-changing process have confirmed a high performance of the proposed controller. It has been emphasized that for its practical usability the system robustification is necessary.
Design and Strength Calculations of the Tripod Support Structure for Offshore Power Plant
Authors: Dymarski C. , Dymarski P. , Żywicki D.J. 
Published: May 2015

Abstract
The support structure being the object of the analysis presented in the article is Tripod. According to the adopted assumptions, it is a foundation gravitationally set in the water region of 60 m in depth, not fixed to the seabed, which can be used for installing a 7MW wind turbine. Due to the lack of substantial information on designing and strength calculations of such types of structures in the world literature, authors have made an attempt to solve this problem within the framework of the abovementioned project. In the performed calculations all basic loads acting on the structure were taken into account, including: the self mass of the structure, the masses of the ballast, the tower and the turbine, as well as hydrostatic forces, and aero- and hydrodynamic forces acting on the entire object in extreme operating conditions.
Application Isssues of the Semi-Markov Reliability Model
Authors: Rudnicki Jacek 
Published: May 2015

Abstract
Predicting the reliability of marine internal combustion engines, for instance, is of particular importance, as it makes it possible to predict their future reliability states based on the information on the past states. Correct reliability prediction is a complex process which consists in processing empirical results obtained from operating practice, complemented by analytical considerations. The process of technical state changes of each mechanical device is stochastic and continuous in states and time, hence the need to divide this infinite set of engine states into a finite number of subsets (classes), which can be clearly and permanently identified using the existing diagnosing system. Using the engine piston-crankshaft system as an example, the article presents a proposal for a mathematical model of reliability which, on the one hand, takes into account random nature of phenomena leading to the damage, and at the same time reveals certain application flexibility and the resultant practical usability.
Impact and Ballistic Resistance of a New Aluminium Alloy for Ship Construction Elements
Authors: Jurczak Wojciech 
Published: May 2015

Abstract
The article presents results of examination of the new alloy 7020M intended to be used in shipbuilding. The examination concerned impact properties, verified on a numerical model, and the resistance to perforation (ballistic resistance). Impact and ballistic resistance tests aimed at increasing the operational safety of marine construc-tions to be made of this alloy under the threat of gunfire or explosion.
Dynamic loading of the alloy 7020M leads to the increase of its strength, as compared to the static load, with the range of this increase depending on the strain rate. Splitting of the structure made of the alloy 7020M increases its impact resistance by three times when preserving the same thickness (g = 12 mm <=> g = 2x6 mm). The alloy examined in the structurally homogenous form revealed low resistance to perforation, while the use of 6 mm thick ballistic ceramic (Al2O3) fully protected this material against damage done by ŁPS 7,62 mm shells having the energy of 3 kJ. The split structure of the alloy ensured better protection against explosive pieces formed during 1,4 kg TNT explosion than the homogeneous structure having the same thickness.
Isolation, identification and screening of Actinobacteria in volcanic soil of Deception Island (the Antarctic) for antimicrobial metabolites
Authors: Cheah Yoke−Kqueen , Lee Learn−Han , Chieng Cheng−Yun Catherine , Wong Vui−Ling Clemente Michael 
Published: May 2015

Abstract
This project aimed to isolate and characterize volcanic soil Actinobacteria from Deception Island, Antarctic. A total of twenty−four Actinobacteria strains were isolated using four different isolation media (Starch casein agar, R2 agar, Actinomycete isolation agar, Streptomyces agar) and characterized basing on 16S rRNA gene sequences. Tests for second− arymetabolites were performed using well diffusion method to detect antimicrobial activities against eight different pathogens, namely Staphyloccocus aureus ATCC 33591, Bacillus megaterium, Enterobacter cloacae, Klebsiella oxytoca, S. enterica serotype Enteritidis, S. enterica serotype Paratyphi ATCC 9150, S. enterica serotype Typhimurium ATCC 14028 and Vibrio cholerae. Antimicrobial properties were detected against Salmonella paratyphi A and Salmonella typhimurium at the concentration of 0.3092±0.08 g/ml. The bioactive strains were identified as Gordonia terrae, Leifsonia soli and Terrabacter lapilli. Results from this study showed that the soil of Deception Island is likely a good source of isolation for Actinobacteria. The volcanic soil Actinobacteria are potentially rich source for discovery of antimicrobial compounds.
Publishing Scientific Journals in the Digital Age: Opportunities for Small Scholarly Journals
Authors: Marušić Ana 
Published: May 2015

Abstract
Scientific publishing is rapidly developing from print-only to digital journals and extensive use of social media. This essay presents our experience in developing a small and scholarly journal from the so-called scientific semi-periphery and using the advantages of digital publishing to increase the visibility of the journal in international indexing and citation databases, and ensure greater visibility in the global scientific community. An editor of a scientific journal does not need to master all details of different digital publishing tools, but needs to follow the developments so that they can be best implemented in the journal.
Recent Advances in the Pathogenesis and Treatment of Chronic Lymphocytic Leukemia
Authors: Efremov Dimitar G. , Laurenti Luca 
Published: May 2015

Abstract
Chronic lymphocytic leukaemia (CLL) is a common lymphoid malignancy characterized by the expansion and progressive accumulation of mature autoreactive B lymphocytes. The disease is clinically heterogeneous and incurable by standard chemotherapy. A major feature of the disease is the marked dependence of the leukaemic cells on various microenvironmental stimuli, which promote leukaemia cell growth, survival, and drug-resistance. Recently, considerable progress has been made in the understanding of the molecular mechanisms that drive CLL. The identification of recurrent genetic lesions using next generation sequencing technology has provided new data on the pathophysiology of the disease and has improved its prognostication. The recognition of the critical role of the B cell receptor (BCR) in driving the disease has resulted in the development of BCR pathway inhibitors that have the potential to completely transform CLL treatment in the near future. Other novel therapeutic agents, such as BCL2 antagonists and chimeric antigen receptor (CAR)-modified T-cells, are also showing great promise in clinical trials. In this review, we summarize some of these recent advances, with a particular focus on the BCR and corresponding pathway inhibitors.
Histological Structure of the Thyroid Gland in Apolipoprotein E Deficient Female Mice After Levothyroxine Application
Authors: Petrova Irena , Mitevska Elida , Gerasimovska Zorica , Milenkova Liljana , Kostovska Nevena 
Published: May 2015

Abstract
The aim of this study was to determine the histological characteristics of the thyroid gland in ApoE KO-/- mice following the application of levothyroxine (l-thyroxine).
A total of 12 female ApoE KO-/- mice were divided into two groups. A control group of mice received distilled water and the experimental group received l-thyroxine dissolved in drinking water at a daily dose of 2 µg/ml, over 12 weeks. The paraffin sections were processed with the usual haematoxilin-eosin technique of staining.
Qualitative histological analysis demonstrated: the presence of large distended follicles in the peripheral areas of the gland; fulfillment of the lumen of follicles with an ample amount of colloid; complete absence of resorptive vacuoles in the colloid; a flattened follicullar epithelium. Morphometric assessment showed a significant increase in the diameters of follicles in the peripheral areas of the gland and a significant decrease in the height of the follicullar epithelium (p <0,001).
Our results demonstrated that l-thyroxine causes characteristic morphological changes in the structure of the thyroid gland in the direction of the occurrence of hyperthyroidism.
Analysis Of Land Use Transformation Potential In Spatial Management
Authors: Kazak Jan , Wang Tong , Szewrański Szymon 
Published: May 2015

Abstract
The current system of spatial planning in Poland does not provide an effective and efficient tool for controlling planning decisions at a level higher than local. The result is an unrealistic approach to adopting development policies. Nowadays there is strong competition among local governments to attract investors, which results in excessive designation of investment areas and, consequently, an imbalance between supply and demand on the real estate market. An extremely important factor from the point of view of local authorities is also the financial burden on government budgets related to the implementation of the provisions of previously adopted policies. An improper spatial development policy can therefore generate costs without delivering the expected results, due to the lack of demand for the offered resources.
A step in the right direction in optimizing how the spatial policy process is shaped may include conducting analyses and forecasts to support the decision-making process. Such analyses are needed both in terms of the amount of areas designed for each type of land use as well as their spatial distribution. Our considerations are focused on the second aspect. Analysis of land use transformation potential can be used in spatial management by selecting areas most where land use is most likely to change. The paper presents the simplified mechanisms of such analyses which can be adopted by the use of cellular automata. The final potential of an area is affected by variables such as the neighborhood, accessibility and suitability. As a result of the integration of these variables, it is possible to determine land use transformation potential. These considerations relate to the MOLAND (Monitoring Land Use/Cover Dynamics) research project and works on the development of the Metronamica decision support system, conducted in Western Europe.