bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa‐Associated Lymphoid Tissue Lymphoma
Authors: Matsui Hidenori , Takahashi Tetsufumi , Øverby Anders , Murayama Somay Yamagata , Yoshida Haruno , Yamamoto Yuji , Nishiyama Keita , Seto Yasuyuki , Takahashi Takashi , Mukai Takao , Nakamura Masahiko 
Published: January 2015

AbstractBackgroundHelicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa‐associated lymphoid tissue (MALT) lymphoma in a C57BL/6J mouse model.Materials and Methods1. C57BL/6J mice were intragastrically administered Lactobacillus strains once daily with 108–109 colony‐forming units (CFU), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 104 copies of 16S rRNA genes) or H. pylori Sydney strain 1 (SS1; 3 × 108 CFU) and continuing for 14 days after infection. 2. C57BL/6J mice were given powdered feed mixed with lyophilized L. gasseri SBT2055 (LG2055) cells (5 × 108 CFU/g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection.Results1. Among the 5 Lactobacillus strains that we examined, only LG2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS1 at day 15 after infection. 2. Dietary supplementation with LG2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY, whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG2055 at 3 months after infection.ConclusionsLG2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.
Pseudoepitheliomatous hyperplasia mimicking invasive squamous cell carcinoma in extranodal natural killer/T‐cell lymphoma: a report of 34 cases
Authors: Ling Yi‐Hong , Zhu Chong‐Mei , Wen Shi‐Hong , Luo Rong‐Zhen , Li Peng , Cao Yun , Rao Hui‐Lan , Lin Su‐Xia , Cai Mu‐Yan 
Published: January 2015

AimsPseudoepitheliomatous hyperplasia (PEH) is defined as a pattern of epidermal reaction. However, it has not yet been extensively documented in extranodal natural killer/T‐cell lymphoma (ENKTL). The aim of our study was to analyse a series of ENKTLs concomitant with PEH mimicking squamous cell carcinoma (SCC).Methods and resultsWe analysed 34 cases of ENKTL with PEH. In our study, the incidence of PEH was 3.8% in ENKTLs diagnosed over a 13‐year period. All 34 cases presented with PEH, appearing as tongue‐like projections of squamous epithelium into the underlying submucosa/dermis with variable depths and jagged borders. The keratinocytes sometimes showed a minor degree of cytological atypia, mostly in the stratum basale, and keratinocyte necrosis was absent. Atypical mitoses and a high nuclear/cytoplasmic ratio were absent. The submucosa and the squamous cell cords were also permeated by atypical lymphocytes.ConclusionsENKTL can be associated with PEH, and the atypical lymphoid cell population can be highly subtle, and therefore may be easily mistaken for SCC, leading to inappropriate therapy. A correct diagnosis requires awareness and recognition of this pitfall by recognizing the associated conditions listed above, which distinguish PEH from SCC.
Handling and reporting of orchidectomy specimens with testicular cancer: areas of consensus and variation among 25 experts and 225 European pathologists
Authors: Berney Daniel M , Algaba Ferran , Amin Mahul , Delahunt Brett , Compérat Eva , Epstein Jonathan I , Humphrey Peter , Idrees Mohammed , Lopez‐Beltran Antonio , Magi‐Galluzzi Cristina , Mikuz Gregor , Montironi Rodolfo , Oliva Esther , Srigley John , Reuter Victor E , Trpkov Kiril , Ulbright Thomas M , Varma Murali , Verrill Clare , Young Robert H , Zhou Ming , Egevad Lars 
Published: January 2015

AimsThe handling and reporting of testicular tumours is difficult due to their rarity.Methods and resultsA survey developed by the European Network of Uro‐Pathology (ENUP) and sent to its members and experts to assess the evaluation of testicular germ cell tumours. Twenty‐five experts and 225 ENUP members replied. Areas of disagreement included immaturity in teratomas, reported by 32% of experts but 68% of ENUP. Although the presence of rete testis invasion was reported widely, the distinction between pagetoid and stromal invasion was made by 96% of experts but only 63% of ENUP. Immunohistochemistry was used in more than 50% of cases by 68% of ENUP and 12% of experts. Staging revealed the greatest areas of disagreement. Invasion of the tunica vaginalis without vascular invasion was interpreted as T1 by 52% of experts and 67% of ENUP, but T2 by the remainder. Tumour invading the hilar adipose tissue adjacent to the epididymis without vascular invasion was interpreted as T1: 40% of experts, 43% of ENUP; T2: 36% of experts, 30% of ENUP; and T3: 24% of experts, 27% of ENUP.ConclusionsThere is remarkable consensus in many areas of testicular pathology. Significant areas of disagreement included staging and reporting of histological types, both of which have the potential to impact on therapy.
Lymph node granulomas in immunoglobulin G4‐related disease
Authors: Bateman Adrian C , Ashton‐Key Margaret R , Jogai Sanjay 
Published: January 2015

AimsImmunoglobulin (Ig)G4‐related disease (IgG4‐RD) is an increasingly recognized fibroinflammatory condition that commonly exhibits multisystem involvement, with localized (e.g. inflammatory pseudotumours that can mimic malignancy) or diffuse (leading to organ dysfunction) patterns of tissue involvement. The 2012 Boston criteria have standardized the histopathological approach to the diagnosis of IgG4‐RD and require one or more of the cardinal morphological features with prominence of IgG4+ plasma cells and an IgG4+/IgG+ plasma cell ratio of at least 40%. The relative prevalence of the morphological criteria varies between anatomical sites, but granulomas are rarely found and, indeed, their presence would usually deter a pathologist from making this diagnosis. The aim was to characterize two cases of IgG4‐RD in which granulomas were present and to highlight this as an unusual feature of the condition.Methods and resultsWe describe two cases in which the features of IgG4‐RD were present within lymph nodes, together with granulomas. This is a recognized but rare morphological pattern of IgG4‐RD.ConclusionsWhile an unusual finding, the presence of granulomas should not preclude a diagnosis of IgG4‐RD in the appropriate clinicopathological context.
Quality of nursing doctoral education in seven countries: survey of faculty and students/graduates
Authors: Kim Mi Ja , Park Chang Gi , McKenna Hugh , Ketefian Shake , Park So Hyun , Klopper Hester , Lee Hyeonkyeong , Kunaviktikul Wipada , Gregg Misuzu F. , Daly John , Coetzee Siedine , Juntasopeepun Phanida , Murashima Sachiyo , Keeney Sinead , Khan Shaheen 
Published: January 2015

AbstractAimsThis study aimed to compare the findings of the quality of nursing doctoral education survey across seven countries and discuss the strategic directions for improving quality.BackgroundNo comparative evaluation of global quality of nursing doctoral education has been reported to date despite the rapid increase in the number of nursing doctoral programmes.DesignA descriptive, cross‐country, comparative design was employed.MethodsData were collected from 2007–2010 from nursing schools in seven countries: Australia, Japan, Korea, South Africa, Thailand, UK and USA. An online questionnaire was used to evaluate quality of nursing doctoral education except for Japan, where a paper version was used. Korea and South Africa used e‐mails quality of nursing doctoral education was evaluated using four domains: Programme, Faculty (referring to academic staff), Resource and Evaluation. Descriptive statistics, correlational and ordinal logistic regression were employed.ResultsA total of 105 deans/schools, 414 faculty and 1149 students/graduates participated. The perceptions of faculty and students/graduates about the quality of nursing doctoral education across the seven countries were mostly favourable on all four domains. The faculty domain score had the largest estimated coefficient for relative importance. As the overall quality level of doctoral education rose from fair to good, the resource domain showed an increased effect.ConclusionsBoth faculty and students/graduates groups rated the overall quality of nursing doctoral education favourably. The faculty domain had the greatest importance for quality, followed by the programme domain. However, the importance of the resource domain gained significance as the overall quality of nursing doctoral education increased, indicating the needs for more attention to resources if the quality of nursing doctoral education is to improve.
Cardiac Resynchronization Therapy Delivered Via a Multipolar Left Ventricular Lead is Associated with Reduced Mortality and Elimination of Phrenic Nerve Stimulation: Long‐Term Follow‐Up from a Multicenter Registry
Authors: BEHAR JONATHAN M. , BOSTOCK JULIAN , ZHU LI ADRIAN PO , CHIN HUI MEN SELINA , JUBB STEPHEN , LENT EDWARD , GAMBLE JAMES , FOLEY PAUL W.X. , BETTS TIM R. , RINALDI CHRISTOPHER ALDO , HERRING NEIL 
Published: January 2015

Lower Mortality and Eliminated PNS Associated with Quadripolar LeadsIntroductionCardiac resynchronization therapy (CRT) using quadripolar left ventricular (LV) leads provides more pacing vectors compared to bipolar leads. This may avoid phrenic nerve stimulation (PNS) and allow optimal lead placement to maximize biventricular pacing. However, a long‐term improvement in patient outcome has yet to be demonstrated.MethodsA total of 721 consecutive patients with conventional CRTD criteria implanted with quadripolar (n = 357) or bipolar (n = 364) LV leads were enrolled into a registry at 3 UK centers. Lead performance and mortality was analyzed over a 5‐year period.ResultsPatients receiving a quadripolar lead were of similar age and sex to those receiving a bipolar lead, although a lower proportion had ischemic heart disease (62.6% vs. 54.1%, P = 0.02). Both groups had similar rates of procedural success, although lead threshold, impedance, and procedural radiation dose were significantly lower in those receiving a quadripolar lead. PNS was more common in those with quadripolar leads (16.0% vs. 11.6%, P = 0.08), but was eliminated by switching pacing vector in all cases compared with 60% in the bipolar group (P < 0.001). Furthermore, LV lead displacement (1.7% vs. 4.6%, P = 0.03) and repositioning (2.0% vs. 5.2%, P = 0.03) occurred significantly less often in those with a quadripolar lead. All‐cause mortality was also significantly lower in the quadripolar compared to bipolar lead group in univariate and multivariate analysis (13.2% vs. 22.5%, P < 0.001).ConclusionsIn a large, multicenter experience, the use of quadripolar LV leads for CRT was associated with elimination of PNS and lower overall mortality. This has important implications for LV pacing lead choice.
Irritability in boys with autism spectrum disorders: an investigation of physiological reactivity
Authors: Mikita Nina , Hollocks Matthew J. , Papadopoulos Andrew S. , Aslani Alexandra , Harrison Simon , Leibenluft Ellen , Simonoff Emily , Stringaris Argyris 
Published: January 2015

BackgroundIrritability in people with autism spectrum disorders (ASD) is common and impairing, yet its mechanisms remain understudied. We investigated symptom reporting and mechanisms of irritability in ASD, focusing on the relation between irritability and physiological stress responses.MethodsForty‐seven unmedicated boys with high‐functioning ASD (hfASD) and 23 typically developing boys aged 10–16 years completed a psychosocial stress test. Changes in cortisol, heart rate and heart rate variability throughout the test were recorded. Self‐ and parent‐reported measures of irritability were obtained. Irritability symptom reporting in the hfASD group was compared to two groups of boys without ASD: highly irritable boys (severe mood dysregulation, SMD; n = 40) and healthy‐control boys (HC; n = 30).ResultsBoys with hfASD scored significantly higher on irritability than HC boys, and they reported a pattern of irritability symptoms closely resembling that of boys with SMD. The internal consistency of irritability in hfASD was high by parent‐ and self‐report. Although boys with hfASD showed significant stress‐induced changes in cortisol and heart rate, those who rated themselves as highly irritable had lower cortisol levels throughout the test compared to those low on irritability. Participants rated as highly irritable by their parents showed blunted cortisol and heart rate responses to stress. The effects of irritability on heart rate, but not cortisol, were accounted for by trait anxiety.ConclusionsIrritability can be measured reliably in hfASD and is associated with distinct biological responses to stress.
Co‐existence of psoriasis and melanoma in a large urban academic centre population: a cross‐sectional retrospective study
Authors: Bhattacharya T. , Nardone B. , Rademaker A. , Martini M. , Amin A. , Al‐Mudaimeagh H.M. , Kiguradze T. , Schneider D. , West D.P. 
Published: January 2015

AbstractBackgroundPsoriasis has been linked to increased malignancy risk, particularly lympho‐haematopoietic and non‐melanoma skin cancers; however, its association with cutaneous melanoma remains unclear.ObjectiveThe aim of this study was to determine if there is an association between melanoma and psoriasis in a large, urban academic population through an electronic medical record database.MethodsWe searched our institution's electronic medical record database (EDW‐Electronic Data Warehouse) from 1/2001 to 11/2013. Subjects were identified by ICD‐9 codes. Melanoma diagnosis was included only if documented at least 1 month after the psoriasis diagnosis was documented. Odds ratio (OR) was obtained for association between cutaneous melanoma and psoriasis. The OR was then adjusted for phototherapy and age. To minimize detection bias, we also obtained the OR for association between cutaneous melanoma and atopic dermatitis.ResultsWe identified 10 947 patients with psoriasis, 64 of whom had a subsequent diagnosis of cutaneous melanoma. We detected a significant association between melanoma and psoriasis (OR = 1.77; 95%CI 1.38–2.26; P < 0.0001; total n = 1 525 252). After adjusting for phototherapy and age, a statistically significant association between melanoma and psoriasis remained detectable (OR = 1.9; 95%CI 1.55–2.55; P < 0.0001 and OR = 1.64; 95%CI 1.17–2.26; P = 0.003 respectively). The OR for melanoma with atopic dermatitis in the same patient database showed a statistically significant inverse association between the two diseases (OR = 0.35; 95%CI 0.16–0.73; P = 0.005).ConclusionOur findings show a statistically significant association between psoriasis and melanoma. After adjusting the OR for phototherapy and age, a statistically significant association remained. Further investigations exploring these associations are warranted in order to establish the relative risk for melanoma in psoriasis patients.
A Preliminary Study on Whether the Soluble Solid Content and Acidity of Oranges Predicted by Near Infrared Spectroscopy Meet the Sensory Degustation
Authors: Yuan Lei‐ming , Sun Li , Cai Jian‐rong , Lin Hao 
Published: January 2015

AbstractInternal qualities of fruit, such as soluble solid content (SSC) and acidity, were mostly used to reflect people's sensory preference. Those indexes have been noninvasively measured by near infrared (NIR) spectroscopy over the past years. However, limited attentions have been given on whether the result of NIR spectroscopy detection can meet the sensory requirement of the consumers. In this research, NIR measurement and the sensory test were carried out on oranges to assay the answer. Multivariate analyses with several pretreatments were comparatively employed to calibrate a robust model on the basis of NIR spectra. The optimal partial least squares model was obtained with root mean square error of prediction of 0.613 for SSC, and 0.17 for acidity. The sensory test was investigated by well‐trained panelists to explore the sensory threshold, which had a root mean square error of deviation of 0.771 for SSC, as well as 0.203 for acidity. Results showed that the noninvasive method of NIR usage was acceptable with fewer root mean square error of prediction value than sensory threshold. This preliminary study revealed that an accurate and fast method by NIR spectrometer coupled with proper chemometric on oranges could meet the requirement of sensory taste.Practical ApplicationsOver the last decades, internal qualities of fruit have been noninvasively detected by NIR spectroscopy, but limited attentions have been given on whether the result of NIR detection can meet the sensory requirement of the consumers. A noninvasive NIR determination method should be deserving of its purpose that can at least meet the sensory taste. In this work, we examined whether the result of NIR application can meet the sensory taste for oranges. The sensory threshold was analyzed and compared with the proposed NIR model, which was simple and robust. It is of great importance to choose a proper NIR model resulting in not only covering the sensory degustation, but also low‐cost and fast detection in the practical implementation. This research will be a good reference for screening and grading of food control or process.
Modified Gelatin Films from Croaker Skins: Effects of pH, and Addition of Glycerol and Chitosan
Authors: Bandeira Sidney Fernandes , da Silva Roberto de Souza Gomes , de Moura Jaqueline Motta , de Almeida Pinto Luiz Antonio 
Published: January 2015

AbstractThis work evaluated the effects of pH (3, 4 and 5) and addition of glycerol (10, 20 and 30%, w/w) in films of gelatin extracted from croaker skins, and afterward modified by chitosan. The gelatin films were produced by casting technique. The chitosan additions (10, 20 and 30% w/w) in relation to the gelatin films were analyzed. Films were characterized as tensile strength (TS), elongation, water vapor permeability (WVP) and optical properties (color, color variation and opacity). Gelatins were characterized for gel strength (221 g) and viscosity (3.7 cP), and chitosan was obtained from shells of shrimp and characterized (150 kDa and deacetylation degree of 85%). The gelatin film with 10% (w/w) glycerol and pH 4 showed the best TS value and suitable WVP value. The amount of chitosan of 10% (w/w) added to the gelatin film on the best conditions produced the modified films with the best properties of TS and WVP.Practical ApplicationsSeveral types of biopolymeric films have been developed, combining proteins and polysaccharides, and among these, gelatin and chitosan take on homogeneous form because of miscibility between the two biopolymers. Improvements in the connection crossed in gelatin mixture with chitosan are attributed to the formation of polielectrolic compounds, among the groups of amino of the chitosan and of the groups of lateral chains charged negatively of the gelatin through electrostatic interaction. The study of the mechanical properties of the biodegradable films is a subject of great importance, because of its influence on the acting of the product and the consumer's acceptance. In this context, the intensification need is observed in the studies about the gelatin extraction starting from fish skins and its application in the production of pure and modified films with chitosan and plasticizer.
The Treatment of keloids and hypertrophic scars with intralesional bleomycin in skin of color
Authors: Payapvipapong Kittisak , Niumpradit Nucha , Piriyanand Chotinand , Buranaphalin Sawanya , Nakakes Artit 
Published: January 2015

SummaryIntralesional injection with corticosteroid remains the mainstay of therapy for hypertrophic scars and keloids, however some lesions are unresponsive or may result in skin atrophy. Intralesional bleomycin injection is an alternative therapy that has been widely reported. In order to compare the effectiveness and safety of bleomycin for the treatment of keloids and hypertrophic scars in skin of color population, Fitzpatrick skin type III to V patients with keloids or hypertrophic scars were randomized into two groups. Group A was treated monthly with intralesional triamcinolone acetonide (10 mg/mL), while group B with intralesional bleomycin (1 mg/mL) for three consecutive months. Evaluation of the treatment was performed using “Patient and Observer Scar Assessment Scale” (POSAS), self‐rated patient satisfaction score, photography, and ultrasonography. Two patients had their bleomycin blood levels monitored. Twenty‐six patients with keloids or hypertrophic scars were recruited. The clinical improvement as assessed by the POSAS was not statistically significant. In terms of patients satisfaction score, one half of both groups reported a very good improvement. Photographic as well as ultrasonographic evaluation showed no difference between the two groups. Bleomycin was found to enter the blood circulation in a very small amount. The major side effect was hyperpigmentation. There was no skin atrophy detected in this study. Intralesional bleomycin is a safe and effective treatment for keloids and hypertrophic scars. The treatment is comparable to intralesional triamcinolone. Unfortunately, hyperpigmentation was the major side effect in darker skin type.
Micronized fibres affect in vitro fermentation under normal buffered and osmotic stress conditions using porcine inocula
Authors: Aumiller T. , Mosenthin R. , Rink F. , Hartung K. , Weiss E. 
Published: January 2015

SummaryIn this in vitro study, the modified Hohenheim gas test was used to determine fermentation activity and bacterial composition of pig's faecal microbial inoculum, when fermenting a standard pig diet with varying levels of crude protein (CP; 20, 24 and 28% CP), and supplemented with one of three fibre sources manufactured by micronization treatment. These were wheat envelopes (MWE), pea fibre (MPF) and lupine fibre (MLF). For comparison, inulin was used. As intestinal bacteria have to cope with varying osmotic conditions in their ecosystem, fermentation was performed under normal buffered and osmotic stress conditions. After 24 h of fermentation, total gas production and ammonia production were measured. In addition, the effect of MWE and inulin on short‐chain fatty acid (SCFA) production and numbers of total eubacteria, Lactobacillus spp., Bifidobacterium spp., Enterobacteriaceae, Enterococcus spp., Clostridium cluster XIVa and Clostridium cluster IV, were determined using quantitative real‐time PCR. Under normal buffered conditions, supplementation of MWE resulted in increased (p < 0.05) SCFA, acetic, propionic and valerianic acid production at CP levels of 20 and 28%. There was an increase (p < 0.05) in ammonia production for the micronized supplements, and for MWE an increased (p < 0.05) branched‐chain proportion was observed, possibly due to higher availability of protein for fermentation which was released during the micronization process. Osmotic stress conditions reduced (p < 0.05) total gas as well as total SCFA, acetic and propionic acid production for all treatments, while cell counts were increased (p < 0.05) for Bifidobacterium spp., Enterococcus spp. and Lactobacillus spp. Under normal buffered conditions in combination with 24 and 28% CP levels, lactobacilli were increased for MWE, compared to inulin (p < 0.05). In conclusion, micronized supplements such as MWE may beneficially modulate pigs' intestinal microbiota by increasing SCFA production in addition to a selective proliferation of lactobacilli.