bluuebunny/crossref_metadata_2025_split huggingface dataset:
MicroRNA-124-3p inhibits cell migration and invasion in bladder cancer cells by targeting ROCK1
Authors: Xu Xianglai , Li Shiqi , Lin Yiwei , Chen Hong , Hu Zhenghui , Mao Yeqing , Xu Xin , Wu Jian , Zhu Yi , Zheng Xiangyi , Luo Jindan , Xie Liping 
Published: November 2013

AbstractBackgroundIncreasing evidence has suggested that dysregulation of certain microRNAs (miRNAs) may contribute to human disease including carcinogenesis and tumor metastasis in human. miR-124-3p is down-regulated in various cancers, and modulates proliferation and aggressiveness of cancer cells. However, the roles of miR-124-3p in human bladder cancer are elusive. Thus, this study was conducted to investigate the biological functions and its molecular mechanisms of miR-124-3p in human bladder cancer cell lines, discussing whether it has a potential to be a therapeutic biomarker of bladder cancer.MethodsThree human bladder cancer cell lines and samples from ten patients with bladder cancer were analyzed for the expression of miR-124-3p by quantitative RT--PCR. Exogenetic overexpression of miR-124-3p was established by transfecting mimics into T24, UM-UC-3 and J82 cells, after that cell proliferation and cell cycle were assessed by MTT assay, flow cytometry and Colony-forming assay. Cell motility and invasion ability were evaluated by wound healing assay and transwell assay. Tissue microarray, and immunohistochemistry with antibodies against ROCK1, MMP2 and MMP9 was performed using the peroxidase and DAB methods. The target gene of miR-124-3p was determined by luciferase assays, quantitative RT--PCR and western blot. The regulation of epithelial-to-mesenchymal transition by miR-124-3p was analyzed by western blot.ResultsmiR-124-3p is frequently down-regulated in bladder cancer both in three bladder cancer cell lines, T24, UM-UC-3, J82 and clinical samples. Overexpression of miR-124-3p induced G1-phase arrest in T24, UM-UC-3 and J82 cell lines and suppressed cell growth in colony-forming assay. miR-124-3p significantly repressed the capability of migration and invasion of bladder cancer cells. In addition, ROCK1 was identified as a new target of miR-124-3p. ROCK1, MMP2, MMP9 were up-regulated in bladder cancer tissues. Furthermore, we demonstrated miR-124-3p could inhibit bladder cancer cell epithelial mesenchymal transfer, and regulated the expression of c-Met, MMP2, MMP9.ConclusionsmiR-124-3p can repress the migration and invasion of bladder cancer cells via regulating ROCK1. Our data indicate that miR-124-3p could be a tumor suppressor and may have a potential to be a diagnostics or predictive biomarker in bladder cancer.
Abnormal expression of paxillin correlates with tumor progression and poor survival in patients with gastric cancer
Authors: Chen Dong-liang , Wang Zhi-qiang , Ren Chao , Zeng Zhao-lei , Wang De-shen , Luo Hui-yan , Wang Feng , Qiu Miao-zhen , Bai Long , Zhang Dong-sheng , Wang Feng-hua , Li Yu-hong , Xu Rui-hua 
Published: November 2013

Abstract

Background
Paxillin (PXN) has been found to be aberrantly regulated in various malignancies and involved in tumor growth and invasion. The clinicopathological and prognostic significance of PXN in gastric cancer is still unclear.

Methods
The expression of PXN was determined in paired gastric cancer tissues and adjacent normal tissues by Western blotting and real-time PCR. Immunohistochemistry was performed to detect the expression of PXN in 239 gastric cancer patients. Statistical analysis was applied to investigate the correlation between PXN expression and clinicopathological characteristics and prognosis in patients. Additionally, the effects of PXN on gastric cancer cell proliferation and migration were also evaluated.

Results
PXN was up-regulated in gastric cancer tissues and cell lines as compared with adjacent normal tissues and normal gastric epithelial cell line GES-1. Overexpression of PXN was correlated with distant metastasis (P = 0.001) and advanced tumor stage (P = 0.021) in gastric cancer patients. Patients with high PXN expression tended to have poor prognosis compared with patients with low PXN expression (P < 0.001). Multivariate analysis demonstrated that PXN expression was an independent prognostic factor (P = 0.020). Moreover, ectopic expression of PXN promotes cell proliferation and migration in AGS cells whereas knockdown of PXN inhibits cell proliferation and migration in SGC7901 cells.

Conclusions
PXN plays an important role in tumor progression and may be used as a potential prognostic indicator in gastric cancer.
Comparing long term impact on ovarian reserve between laparoscopic ovarian cystectomy and open laprotomy for ovarian endometrioma
Authors: Zaitoun Moustafa M , Zaitoun Mohamed Moustafa , El Behery Manal M 
Published: November 2013

Abstract

Objective
To compare the long term impact on ovarian reserve between laparoscopic ovarian cystectomy with bipolar electrocoagulation and laparotomic cystectomy with suturing for ovarian endometrotic cyst.

Patient and method(s)
121 patients with benign ovarian endometroitic cysts were randomised to either laparoscopic ovarian cystectomy using bipolar electrocoagulation (61 patients) or laparotomic ovarian cystectomy using sutures (60 patients). Serum follicle-stimulating hormone, Antimullerian hormon, Basal antral follicle Count, mean ovarian diameter, and ovarian stromal blood flow velocity were measured at 6, 12 and 18 months after surgery and compared in both groups.

Result(s)
A statistically significant increase of serum FSH was found in the laproscopic bipolar group at 6-, 12 and 18-month postoperativly compared to open laparotomy suture group. Also, a statistically significant decrease of the mean AMH value occurred in laproscopic bipolar group at 6-, 12 and 18-month follow- up compared to open laparotomy suture group. Basal antral follicle number, mean ovarian diameter and peak systolic velocity were significantly decreased during the 6-, 12,18 -month follow-up in laproscopic bipolar group compared to open laparotomy suture group.

Conclusion(s)
After laproscopic ovarian cystecomy for endometrioma all pareameter of ovarian reseve are significantly decreased on long term follow up as compared to open laprotomy.
HLA Class II Haplotypes Differentiate Between the Adult Autoimmune Polyglandular Syndrome Types II and III
Authors: Flesch B. K. , Matheis N. , Alt T. , Weinstock C. , Bux J. , Kahaly G. J. 
Published: November 2013

Background:
Genetics of the adult autoimmune polyglandular syndrome (APS) is poorly understood.

Aim:
The aim of this study was to gain further insight into the genetics of the adult APS types.

Site:
The study was conducted at a university referral center.

Methods:
The human leukocyte antigen (HLA) class II alleles, haplotypes, and genotypes were determined in a large cohort of patients with APS, autoimmune thyroid disease (AITD), and type 1 diabetes and in healthy controls by the consistent application of high-resolution typing at a four-digit level.

Results:
Comparison of the allele and haplotype frequencies significantly discriminated patients with APS vs AITD and controls. The HLA class II alleles DRB1*03:01 *04:01, DQA1*03:01, *05:01, DQB1*02:01, and *03:02 were observed more frequently (P &lt; .001) in APS than in AITD and controls, whereas the alleles DRB1*15:01, DQB1*03:01, and *06:02 were underrepresented in APS vs AITD (Pc &lt; .001) and controls (Pc &lt; .01), respectively. The DRB1*03:01-DQA1*05:01-DQB1*02:01 (DR3-DQ2) and DRB1*04:01-DQA1*03:01:DQB1*03:02 (DRB1*04:01-DQ8) haplotypes were overrepresented in APS (Pc &lt; .001). Combination of both haplotypes to a genotype was highly prevalent in APS vs AITD and controls (Pc &lt; .001). Dividing the APS collective into those with Addison's disease (APS type II) and those without Addison's disease but including type 1 diabetes and AITD (APS type III) demonstrated DR3-DQ2/DRB1*04:01-DQ8 as a susceptibility genotype in APS III (Pc &lt; .001), whereas the DR3-DQ2/DRB1*04:04-DQ8 genotype correlated with APS II (Pc &lt; .001). The haplotypes DRB1*11:01-DQA1*05:05-DQB1*03:01 and DRB1*15:01-DQA1*01:02-DQB1*06:02 are protective in APS III but not in type II (Pc &lt; .01).

Conclusions:
HLA class II haplotypes differentiate between the adult APS types II and III. Susceptible haplotypes favor the development of polyglandular autoimmunity in patients with AITD.
Subclinical Hypothyroidism and Left Ventricular Mechanics: A Three-Dimensional Speckle Tracking Study
Authors: Tadic Marijana , Ilic Sanja , Kostic Nada , Caparevic Zorica , Celic Vera 
Published: November 2013

Context:
Subclinical hypothyroidism (SHT) is associated with left ventricular (LV) remodeling. The LV mechanics has not been previously assessed by two- and three-dimensional (2DE and 3DE) speckle tracking imaging in the SHT patients.

Objectives:
The objective of the study was to investigate LV mechanics by 2DE and 3DE speckle tracking in the SHT patients and evaluate the influence of levothyroxine therapy on LV remodeling.

Design:
We conducted a prospective study. All SHT patients received levothyroxine therapy and were followed up for 1 year after the euthyroid state had been achieved.

Setting:
The study was performed at a university hospital.

Patients:
We included 54 untreated women with SHT and 40 healthy control women who were of similar age.

Main Outcome Measures:
The 2DE strain and strain rates, 3DE volumes, 3DE strain, and thyroid hormones levels were assessed.

Results:
The 2DE LV longitudinal and circumferential strain and systolic and early diastolic strain rates were significantly decreased in the SHT patients before therapy in comparison with the controls or the SHT patients after therapy. The 3DE LV cardiac output and ejection fraction were significantly reduced in the SHT patients at baseline compared with the controls or patients after 1 year of treatment. The 3DE LV longitudinal and radial strains were significantly lower in the SHT group before treatment in comparison with the controls or patients after therapy, whereas the 3DE LV circumferential and area strains gradually increased from untreated SHT patients, among the treated SHT patients, to the controls.

Conclusion:
SHT significantly affects LV deformation assessed by 2DE and 3DE speckle tracking. The improvement of LV mechanics after 1 year of levothyroxine treatment is significant but incomplete.
Spatial and temporal variation in the hydrodynamic landscape in intertidal salt marsh systems
Authors: Wilson Miranda L. , Webster Donald R. , Weissburg Marc J. 
Published: November 2013

Lay AbstractThe spatial and temporal variability of water flow at the scale of ecological processes in aquatic systems such as estuaries is not well understood. Current sampling schemes used to assess the impact of water flow on ecological processes are limited in their ability to encompass adequate spatial and temporal variability, confound spatial and temporal patterning, and do not provide clear connections to values of turbulent flow parameters. We examined spatial and temporal variation of water flow within and among four sites in Wassaw Sound, Georgia, USA, corresponding to different tidal types (i.e., neap, mean, and spring tides). Variation in water flow within the sites was similar and highly correlated at locations up to 20 m but was highly variable among distant sites. Some flow parameters also showed strong dependence on the site. Furthermore, flow parameters did not correlate with each other, indicating that specific parameters need to be identified to correctly assess the effect of water flow on ecological interactions. We provide several suggestions to improve current sampling schema, which include taking flow measurements concurrently with ecological experimentation, minimizing sequential sampling designs, and sampling water flow at similar spatial and temporal scales as ecological experimentation. Consideration of these issues should provide a better way in which to assess the interaction between water flow and ecological processes and help generate testable hypotheses that incorporate how these interactions may vary spatially and temporally.
Ionic versus Electronic Ferroelectricity in Donor–Acceptor Molecular Sequences
Authors: Horiuchi Sachio , Kobayashi Kensuke , Kumai Reiji , Ishibashi Shoji 
Published: November 2013

Abstract
Molecular displacement in an alternating chain of donors and acceptors triggers ferroelectric switching of large electric polarization with a low electric field in some charge-transfer complexes. While the displacement of a point charge (i.e., static charge) provides a good picture of the polarization for spin-Peierls-type transition in an ionic complex of tetrathiafulvalene (TTF) and p-bromanil (BA), it completely fails to explain both the magnitude and direction of the observed spontaneous polarization in the neutral-ionic transition of TTF–p-chloranil (CA). The most spectacular behavior is that the TTF cations are displaced toward the anode and the CA anions toward the cathode. Recent experimental and theoretical research has highlighted the strong effects of dynamic electrons traveling in the intermolecular space of TTF–CA. The new “electronic ferroelectricity” mechanism can also be envisioned as a powerful guide for guaranteeing high-performance dielectric and related electronic functionalities, especially for organic molecular systems.
Surface Modification Method for Adhesion of Gels
Authors: Abe Hidekazu , Hara Yusuke , Maeda Shingo , Hashimoto Shuji 
Published: November 2013

Abstract
We successfully realized the adhesion of cationic gels in aqueous solution by a method of surface modification with an anionic polymer chain. The adhesion force of the gel, which originates in the electrostatic interaction between the cationic gel surface and anionic polymer chain, is controlled by the solution pH because poly(acrylic acid) with –COO− and poly(N,N′-dimethylaminopropylacrylamide) gel with –NH+(CH3)2 were used. With the proposed method, we can select the electric charge of each gel surface to adhere to anionic or cationic gels.
Non-invasive, non-radiological quantification of anteroposterior knee joint ligamentous laxity
Authors: Russell D. F. , Deakin A. H. , Fogg Q. A. , Picard F. 
Published: November 2013

Objectives We performed in vitro validation of a non-invasive skin-mounted system that could allow quantification of anteroposterior (AP) laxity in the outpatient setting. Methods A total of 12 cadaveric lower limbs were tested with a commercial image-free navigation system using trackers secured by bone screws. We then tested a non-invasive fabric-strap system. The lower limb was secured at 10° intervals from 0° to 60° of knee flexion and 100 N of force was applied perpendicular to the tibia. Acceptable coefficient of repeatability (CR) and limits of agreement (LOA) of 3 mm were set based on diagnostic criteria for anterior cruciate ligament (ACL) insufficiency. Results Reliability and precision within the individual invasive and non-invasive systems was acceptable throughout the range of flexion tested (intra-class correlation coefficient 0.88, CR 1.6 mm). Agreement between the two systems was acceptable measuring AP laxity between full extension and 40° knee flexion (LOA 2.9 mm). Beyond 40° of flexion, agreement between the systems was unacceptable (LOA > 3 mm). Conclusions These results indicate that from full knee extension to 40° flexion, non-invasive navigation-based quantification of AP tibial translation is as accurate as the standard validated commercial system, particularly in the clinically and functionally important range of 20° to 30° knee flexion. This could be useful in diagnosis and post-operative evaluation of ACL pathology. Cite this article: Bone Joint Res 2013;2:233–7.
Urinary Cannabinoid Disposition in Occasional and Frequent Smokers: Is THC-Glucuronide in Sequential Urine Samples a Marker of Recent Use in Frequent Smokers?
Authors: Desrosiers Nathalie A , Lee Dayong , Concheiro-Guisan Marta , Scheidweiler Karl B , Gorelick David A , Huestis Marilyn A 
Published: November 2013

Abstract

BACKGROUND
There is extended urinary excretion of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH) in abstinent frequent cannabis smokers. We characterized THC, 11-OH-THC, THCCOOH, cannabidiol, cannabinol, THC-glucuronide, and THCCOOH-glucuronide disposition in urine of frequent and occasional cannabis smokers, and we propose a model to predict recent cannabis smoking.

METHODS
Frequent and occasional smokers resided on a closed research unit and smoked one 6.8% THC cannabis cigarette ad libitum. Urinary cannabinoids were quantified in each void by liquid chromatography-tandem mass spectrometry within 24 h of collection.

RESULTS
No urine samples had measureable THC, 11-OH-THC, cannabidiol, or cannabinol. THCCOOH, THC-glucuronide, and THCCOOH-glucuronide were measurable in all frequent smokers' urine and 60%, 100%, and 100% of occasional smokers' urine samples, respectively. Pre- and postdose maximal concentrations (non- and creatinine normalized) and probability of being positive were significantly higher in frequent smokers' samples. THC-glucuronide concentrations peaked 0.6–7.4 h after smoking; THCCOOH and THCCOOH-glucuronide concentrations were highly variable. At the newly adopted THCCOOH 175-μg/L World Anti-Doping Agency decision limit, only 50% of frequent smokers were positive 0–6 h postdose; no occasional smokers' samples were positive. An absolute %difference of ≥50% between 2 consecutive THC-glucuronide–positive samples with a creatinine-normalized concentration of ≥2 μg/g in the first sample predicted cannabis smoking with efficiencies of 93.1% in frequent and 76.9% in occasional smokers within 6 h of first sample collection.

CONCLUSIONS
These controlled urinary cannabinoid data provide a possible means of identifying recent cannabis intake in cannabis smokers' urine within a short collection time frame after smoking.
A critical comparison of methods for evaluating Arts and Dementia programmes
Authors: Goulding Anna 
Published: November 2013

Abstract
The aim of providing a close reading of a randomized controlled trial (RCT) of reminiscence groups for people with dementia and an evaluation of a pilot arts programme for people with dementia is to compare evidence from the paradigms of health and arts. Whilst the RCT is the gold standard of research in Health, the qualitative approach favoured by arts may be able to capture subtle shifts in participants’ affect. Although RCTs are beyond the budget of small arts organizations, there are lessons to be learnt in terms of study design – the use of control groups, random allocation, the blinding of researchers to the intervention participants receive, regular data collection points and the use of validated quantitative scales. There is already considerable pressure on small arts organizations to provide effective evaluation that satisfies a range of stakeholders, and adopting the suggested methodologies would require considerable support in terms of resource and expertise. While the focus of this article is on arts and health work for older people, the principal of comparing a RCT with a general evaluation has relevance for arts programmes with health outcomes for a range of participants.
A return to ordinariness: How does working alongside people who use mental health service effect theatre students’ attitudes to mentalillness?
Authors: Rowe Nick , Forshaw Nicola , Alldred Gemma 
Published: November 2013

Abstract
This article reports on the experiences of a group of five undergraduate theatre students working alongside people who use mental health services. Our aim was to study any changes in students’ attitudes over the period when they were making theatre with people with mental health problems. At first students were keen to define ‘mental illness’ and to draw clear lines between people who had or did not have mental health problems. They were likely to notice differences and attribute these to mental illness and they were keen to understand how they could help people through theatre. The most striking consequence of students’ contact was a change from regarding people who use mental health services as ontologically different, highly vulnerable and in need of special care and treatment to engaging with them as theatre makers and learners: a relationship and a purpose far more familiar to students. We have called this ‘a return to ordinariness’.
Improvising on the ward: Exploring somatic dance and potential in paediatric health care
Authors: Dowler Lisa 
Published: November 2013

Abstract
This article documents the pilot From Where You Are project, which researched the effects of improvised, somatic dance practice with children and young people on the Neuromedical and Oncology wards of Alder Hey Children’s Hospital (AHCH), UK, in 2008–2009. It considers how the non-judgemental, inclusive and implicitly phenomenological methodology of dance improvisers, whose practice is informed by somatic and environmental approaches, is particularly appropriate and successful in a clinical setting. This is substantiated through an interpretive phenomenological approach, incorporating first-person description and case studies, and provides tangible evidence of the benefits of this practice in paediatric health care.
Reperforming memories: Using the reminiscence theatre archive as a resource for new intergenerational work
Authors: Lilley Heather , Derbyshire Harry 
Published: November 2013

Abstract
In 2012 the University of Greenwich acquired the reminiscence theatre archive of Pam Schweitzer, founder and former Artistic Director of Age Exchange Theatre Company. This article is an account of our first performance project using the archive in collaboration with student volunteers and Schweitzer herself, to explore the possibilities for activating the archive materials through reperformance. Through a process of devising new plays from the archived transcripts of elderly people’s memories, and then performing them in local Sheltered Housing units, the project was designed to enable an assessment of what ongoing benefits the archive might bring to the teaching, research, student experience and community engagement activities of the drama programme. The project has offered indications as to how Schweitzer’s well-known reminiscence theatre methods can be adapted for working with archived materials; and the research findings suggest some of the challenges and possible new directions that might be taken, in the continued reuse of the Reminiscence Theatre Archive.
Reviews
Authors: Watley George , Blatner Adam , Kossak Mitchell , Hickley Niall 
Published: November 2013

Abstract
Theatre, Education and Performance, Helen Nicholson (2011) Basingstoke: Palgrave Macmillan, 245 pp., ISBN: 9780230574236, p/bk, £17.99
Theatre for Change: Education, Social Action and Therapy, Robert J. Landy and David T. Montgomery (2012) New York: Palgrave Macmillan, 337 pp., ISBN: 978-0-230-24366-8, p/bk, $30.00
Expressive Arts in the Winds of Change, 10th International Conference of the International Expressive Arts Therapy Association, Double Tree Hotel, Berkeley, CA, 14–17 March 2013
Exploring where Poetry and Therapy Intersect: Two workshops delivered by Diana Hedges and Reamonn O’Donnchadha, PoetryReach, Maynooth, County Kildare, Ireland, 23 March 2013