bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix metalloproteinase‐7 and matrix metalloproteinase‐25 in oral tongue squamous cell carcinoma
Authors: Mäkinen Laura K. , Häyry Valtteri , Hagström Jaana , Sorsa Timo , Passador–Santos Fabricio , Keski–Säntti Harri , Haukka Jari , Mäkitie Antti A. , Haglund Caj , Atula Timo 
Published: November 2013

AbstractBackgroundPredicting the clinical course of early‐stage oral tongue squamous cell carcinoma (SCC) is challenging. As matrix metalloproteinases (MMPs) are enzymes associated with invasion, metastasis, and poor survival in many cancers, we examined MMP‐7 and MMP‐25 in oral tongue SCC.MethodsWe used tissue microarray (TMA) technique and immunohistochemistry to study the expression of MMP‐7 and MMP‐25 in 73 patients with stage I to II oral tongue SCC and compared their immunoexpressions with clinical data.ResultsImmunohistochemistry revealed MMP‐7 and MMP‐25 expression in 90% (n = 63 of 70) and 90% (n = 64 of 71) of the tumors, respectively. MMP‐7 protein expression was associated with presence of occult cervical metastases (odds ratio [OR], 3.67; p = .013), increased invasion depth (OR, 4.60; p = .005), and higher tumor grade (OR, 3.30; p = .007). MMP‐7 expression was predictive for poor outcome (p = .021). Immunostaining of MMP‐25 did not correlate with any clinical parameters.ConclusionWe conclude that MMP‐7, but not MMP‐25, expression may have prognostic significance in early‐stage oral tongue SCC. © 2014 Wiley Periodicals, Inc. Head Neck 36: 1783–1788, 2014
Mesenchymal stem cell response to conformal sputter deposited calcium phosphate thin films on nanostructured titanium surfaces
Authors: McCafferty Mura M. , Burke George A. , Meenan Brian J. 
Published: November 2013

AbstractBiomaterial surfaces that can directly induce the osteogenic differentiation of mesenchymal stem cells (MSCs) present an exciting strategy for bone tissue engineering and offers significant benefits for improving the repair or replacement of damaged or lost bone tissue. In this study, titanium nanostructures with distinctive topographical features were produced by radio frequency magnetron sputtering. The response of MSCs to the nanostructured titanium (Ti) surfaces before and after augmentation by a sputter deposited calcium phosphate (CaP) coating has been investigated. The sputtered CaP has the characteristics of a calcium enriched hydroxyapatite surface layer, as determined by X‐ray photoelectron spectroscopy and X‐ray diffraction studies. The sputter deposited Ti has a polycrystalline surface morphology, as confirmed by atomic force microscopy, and CaP layers deposited thereon (TiCaP) conform to this topography. The effects of these surfaces on MSC focal adhesion formation, actin cytoskeleton organization and Runx2 gene expression were examined. The Ti and TiCaP surfaces were found to promote changes in MSC morphology and adhesion known to be associated with subsequent downstream osteogenic differentiation; however, the equivalent events were not as pronounced on the CaP surface. A significant increase in Runx2 expression was observed for CaP compared to Ti, but no such difference was seen between either Ti and TiCaP, nor CaP and TiCaP. Importantly, the Ti surface engendered the expected contribution of nanoscale features to the MSC response; moreover, the CaP layer when used in combination with this topography has been found to cause no adverse effects in respect of MSC behavior. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3585–3597, 2014.
Heparin modification of a biomimetic bone matrix for controlled release of VEGF
Authors: Knaack Sven , Lode Anja , Hoyer Birgit , Rösen‐Wolff Angela , Gabrielyan Anastasia , Roeder Ingo , Gelinsky Michael 
Published: November 2013

AbstractBone regeneration using tissue engineered constructs requires strategies to effectively stimulate vascularization within such a construct that is crucial for its supply and integration with the host tissue. In this work, porous scaffolds of a collagen/hydroxyapatite nanocomposite were modified with heparin to generate biomimetic bone matrices which are able to release angiogenic factors in a controlled manner. Heparin was either integrated during material synthesis (in situ) or added to the scaffolds after their fabrication (post). Both approaches resulted in stable incorporation of heparin into the matrix of mineralized collagen. Investigations of binding and release of the vascular endothelial growth factor (VEGF‐A165) loaded onto the scaffolds revealed an enhanced binding capacity as well as a sustained and nearly constant delivery of VEGF as result of both heparin modification methods. The release rate could be controlled by varying the quantity of incorporated heparin and the modification method. Although the biological activity of VEGF released after 7 days from the unmodified scaffolds was reduced in comparison to control VEGF, it was maintained after release from post or even enhanced after release from in situ modified scaffolds. In conclusion, the heparin‐modified scaffolds of mineralized collagen exhibited favorable growth factor binding and release properties and may be beneficial to stimulate vascularization. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3500–3511, 2014.
Titania nanotubes dimensions‐dependent protein adsorption and its effect on the growth of osteoblasts
Authors: Yang Weihu , Xi Xingfeng , Shen Xinkun , Liu Peng , Hu Yan , Cai Kaiyong 
Published: November 2013

AbstractIn this study, we report the influence of titania nanotubes (TiNTs) dimensions on the adsorption of collagen (COL) and fibronectin (FN), and its subsequent effect on the growth of osteoblasts. TiNTs with different diameters of around 30 and 100 nm were prepared with anodization. The adsorption profiles of proteins and cell behaviors were evaluated using spectrophotometric measurement, immunofluorescence staining, cell viability, and cytoskeleton morphology, respectively. The results showed that although the growth of osteoblasts was highly sensitive to the dimensions TiNTs, the preadsorbed COL and FN could reduce the difference. Molecular dynamics (MD) simulation results confirmed that the main driving force for protein adsorption was the physical adsorption. The TiNTs with bigger dimensions had higher interaction energies, and thus leading to higher proteins (COL and FN) adsorption and obvious influences on cell behaviors. MD simulation revealed that the orientation and conformation of proteins adsorbed onto surfaces of TiNTs was critical for cell integrins to recognize specific sites. When FN molecules adsorbed onto the surfaces of TiNTs, their RGD (Arg‐Gly‐Asp) sites were easily exposed to outside and more likely to bond with the fibronectin receptors, in turn regulating the cellular behaviors. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 102A: 3598–3608, 2014.
Lactobacillus plantarumCECT 7527, 7528 and 7529: probiotic candidates to reduce cholesterol levels
Authors: Bosch Montserrat , Fuentes Mari C , Audivert Sergi , Bonachera Miquel A , Peiró Sara , Cuñé Jordi 
Published: November 2013

AbstractBACKGROUNDHypercholesterolaemia plays a key role in the development and progression of coronary artery disease. There are numerous drugs available to treat this condition but they are often expensive and can have unwanted side effects. Therefore, a screening of lactic acid bacteria to select candidate probiotic strains to reduce cholesterol levels was carried out. Three Lactobacillus plantarum strains (CECT 7527, 7528 and 7529) were selected as potential probiotics to reduce cholesterol levels after conducting several in vitro tests for demonstrating the functionalities of the strains according to international guidelines.RESULTSThe three strains showed a high ability to survive under gastrointestinal tract conditions and to adhere to intestinal cells. Regarding lipid metabolism, the strains showed a great production of bile salt hydrolase, especially when combined. Moreover, the strains assimilated cholesterol directly from the medium. Part of the cholesterol present in the medium was removed via binding onto the bacterial cellular surface. Finally, the three strains, especially CECT 7529, produce large quantities of propionic and butyric acids.CONCLUSIONCombined, these characteristics suggest that these strains could be excellent candidates for reducing high blood cholesterol levels. © 2013 Society of Chemical Industry
Perceived vocal fatigue and effort in relation to laryngeal functional measures in paresis patients
Authors: Stager Sheila V. , Bielamowicz Steven A. 
Published: November 2013

Objectives/HypothesisTo determine if differences in objective measures of laryngeal function can meaningfully explain different levels of self‐perceptions of effort or fatigue in patients with vocal fold paresis.Study DesignA retrospective chart review of 72 patients with vocal fold paresis diagnosed using laryngeal electromyography, who had either been observed (n = 21), treated only by injection (n = 24), or treated only by surgery (n = 27).MethodsBefore and after treatment/observation, patients' subjective ratings of severity of vocal effort and fatigue were assessed using the Glottal Function Index. Laryngeal function was assessed using maximum phonation time and translaryngeal flow.ResultsNone of the variables demonstrated a significant linear change across time. Post hoc Tukey analyses following analysis of variance (ANOVA) found significant differences in flow among three groups, those rating symptoms of effort as no problem, moderate problem, or severe problem. Post hoc Tukey analyses following ANOVA found significant differences in the amount that flow changed among three groups, those demonstrating no difference, minor differences, or major differences in ratings of effort before and after treatment.ConclusionsChanges in reported symptom severity of effort were related to changes in translaryngeal midvowel flow that were not explained by passage of time.Level of Evidence4. Laryngoscope, 124:1631–1637, 2014
Identification of carotenoids using mass spectrometry
Authors: Rivera Sol M. , Christou Paul , Canela‐Garayoa Ramon 
Published: November 2013

AbstractThe present review compiles positive MS fragmentation data of selected carotenoids obtained using various ionization techniques and matrices. In addition, new experimental data from the analysis of carotenoids in transgenic maize and rice callus are provided. Several carotenes and oxygen‐functionalized carotenoids containing epoxy, hydroxyl, and ketone groups were ionized by atmospheric pressure chemical ionization (APCI)‐tandem mass spectrometry (MS/MS) in positive ion mode. Thus, on the basis of the information obtained from the literature and our own experiments, we identified characteristic carotenoid ions that can be associated to functional groups in the structures of these compounds. In addition, pigments with a very similar structure were differentiated through comparison of the intensities of their fragments. The data provide a basis for the structural elucidation of carotenoids by mass spectrometry (MS). © 2013 Wiley Periodicals, Inc. Mass Spec Rev 33: 353–372, 2014
Strategic Involuntary Teacher Transfers and Teacher Performance: Examining Equity and Efficiency
Authors: Grissom Jason A. , Loeb Susanna , Nakashima Nathaniel A. 
Published: November 2013

AbstractDespite claims that school districts need flexibility in teacher assignment to allocate teachers more equitably across schools and improve district performance, the power to involuntarily transfer teachers across schools remains hotly contested. Little research has examined involuntary teacher transfer policies or their effects on schools, teachers, or students. This article uses administrative data from Miami‐Dade County Public Schools to investigate the implementation and effects of the district's involuntary transfer policy, including which schools transferred and received teachers, which teachers were transferred, what kinds of teachers replaced them in their former schools, and how their performance—as measured by their work absences and value‐added in math and reading—compared before and after the transfer. We find that, under the policy, principals in the lowest performing schools identified relatively low‐performing teachers for transfer who, based on observable characteristics, would have been unlikely to leave on their own. Consistent with an equity improvement, we find that involuntarily transferred teachers were systematically moved to higher performing schools and generally were outperformed by the teachers who replaced them. Efficiency impacts are mixed; although transferred teachers had nearly two fewer absences per year in their new positions, transferred teachers continued to have low value‐added in their new schools.
Analysis of serum insulin growth factor‐1 concentrations in localized osteosarcoma: A children's oncology group study
Authors: Borinstein Scott C. , Barkauskas Donald A. , Bernstein Mark , Goorin Allen , Gorlick Richard , Krailo Mark , Schwartz Cindy L. , Wexler Leonard H. , Toretsky Jeffrey A. 
Published: November 2013

AbstractTo investigate the role of insulin‐like growth factor‐1 (IGF‐1), in localized osteosarcoma, serum levels of IGF‐1, IGFBP‐2, and IGFBP‐3 were measured in 224 similarly treated, newly diagnosed patients. We demonstrated that younger patients had lower concentrations of IGF‐1 and IGFBP‐3 compared to older (P < 0.001) along with lower IGFBP‐3:IGF‐1 and IGFBP‐2:IGF‐1 ratios (P < 0.001). IGFBP‐2 did not correlate with age (P = 0.16), yet IGFBP‐2:IGF‐1 ratios were higher in the younger population (P < 0.001). These findings show that older patients have higher concentrations of free IGF‐1. None of IGF‐1, IGFBP‐2, nor IGFBP‐3 concentrations were associated with event‐free nor overall survival. Pediatr Blood Cancer 2014;61:749–752. © 2013 Wiley Periodicals, Inc.
Concordance between the chang and the International Society of Pediatric Oncology (SIOP) ototoxicity grading scales in patients treated with cisplatin for medulloblastoma
Authors: Bass Johnnie K. , Huang Jie , Onar‐Thomas Arzu , Chang Kay W. , Bhagat Shaum P. , Chintagumpala Murali , Bartels Ute , Gururangan Sridharan , Hassall Tim , Heath John A. , McCowage Geoffrey , Cohn Richard J. , Fisher Michael J. , Robinson Giles , Broniscer Alberto , Gajjar Amar , Gurney James G. 
Published: November 2013

AbstractBackgroundReporting ototoxicity is frequently complicated by use of various ototoxicity criteria. The International Society of Pediatric Oncology (SIOP) ototoxicity grading scale was recently proposed for standardized use in reporting hearing loss outcomes across institutions. The aim of this study was to evaluate the concordance between the Chang and SIOP ototoxicity grading scales. Differences between the two scales were identified and the implications these differences may have in the clinical setting were discussed.ProceduresAudiological evaluations were reviewed for 379 patients with newly diagnosed medulloblastoma (ages 3–21 years). Each patient was enrolled on one of two St. Jude clinical protocols that included craniospinal radiation therapy and four courses of 75 mg/m2 cisplatin chemotherapy. The latest audiogram conducted 5.5–24.5 months post‐protocol treatment initiation was graded using the Chang and SIOP ototoxicity criteria. Clinically significant hearing loss was defined as Chang grade ≥2a and SIOP ≥2. Hearing loss was considered serious (requiring a hearing aid) at the level of Chang grade ≥2b and SIOP ≥3.ResultsA strong concordance was observed between the Chang and SIOP ototoxicity scales (Stuart's tau‐c statistic = 0.89, 95% CI: 0.86, 0.91). Among those patients diagnosed with serious hearing loss, the two scales were in good agreement. However, the scales deviated from one another in classifying patients with less serious or no hearing loss.ConclusionsAlthough discrepancies between the Chang and SIOP ototoxicity scales exist primarily for patients with no or minimal hearing loss, the scales share a strong concordance overall. Pediatr Blood Cancer 2014;61:601–605. © 2013 Wiley Periodicals, Inc.
Correlation analysis of ductus venosus velocity indices and fetal cardiac function
Authors: Sanapo L. , Turan O. M. , Turan S. , Ton J. , Atlas M. , Baschat A. A. 
Published: November 2013

ABSTRACTObjectiveTo examine the relationships between the ductus venosus (DV) pulsatility index for veins (PIV), individual DV velocity ratios and diastolic and global myocardial cardiac function.MethodsDoppler measurements of the DV, atrioventricular (AV) valves and ventricular in‐ and outflow were analyzed. The DV‐PIV and velocity ratios for individual phases (systole (S), end‐systolic relaxation (v), early diastole (D), atrial systole (a), and S/v, S/D, S/a, v/D, v/a and D/a ratios) were calculated. The ratio of early and late diastolic peak velocities across AV valves was calculated (E/A ratio). Left modified myocardial performance index (MPI) was calculated from time intervals between valve clicks defining isovolumetric contraction/relaxation and ejection times. All values were transformed to Z‐scores. The distributions of DV velocity ratios and DV‐PIV were correlated with cardiac Doppler parameters.ResultsA total of 1163 examinations from 213 fetuses, most of which were at risk for cardiac dysfunction, were included in the study. In 742 the PIV was normal and in 421 PIV was elevated > 2 SD above the normal mean. The DV‐PIV correlated with velocity ratios (P < 0.0001) but not with E/A ratios and the MPI. S/v and v/D ratios were related to tricuspid and mitral E/A ratios and left ventricular MPI. The S/D ratio was only related to both E/A ratios. There was no relationship between a‐wave‐related velocity ratios and cardiac function.ConclusionsVelocity ratios of the DV show relationships with cardiac function that are not reflected by the PIV alone. In cases of suspected fetal cardiac dysfunction based on elevated DV‐PIV, analysis of velocity ratios or direct cardiac evaluation is suggested to determine the underlying pathophysiology. Copyright © 2013 ISUOG. Published by John Wiley & Sons Ltd.
The effect of frequency of telemetry locations on movement‐rate calculations in arctic caribou
Authors: Prichard Alexander K. , Yokel David A. , Rea Caryn L. , Person Brian T. , Parrett Lincoln S. 
Published: November 2013

AbstractThere is a trade‐off between the frequency of telemetry locations (fix interval) and battery life when using Global Positioning System (GPS) collars. In general, decreasing the fix interval lowers the effective battery life of the collar. However, the strong relationship between fix interval and movement metrics is often underappreciated. Mean movement rates, maximum movement rates, total distance estimates, and some screening algorithms change dramatically with different fix intervals, particularly for highly mobile animals such as caribou (Rangifer tarandus), and must be interpreted in relation to the fix interval used to collect the data. We used a multi‐year, high‐resolution data set from GPS collars with 2‐hr fix intervals deployed on female caribou of the Teshekpuk Caribou Herd, Alaska, USA, 2006–2011, to examine the effect of fix interval on estimates of movement metrics. By calculating straight‐line distances between locations taken at different fix intervals, we examined the rate of change in various metrics of caribou movement as a function of fix interval. We also calculated correction factors for different fix intervals, and examined how they changed seasonally. We fit an equation to the maximum rate of movement of caribou as a function of fix interval and used this equation in a modification of one screening algorithm to assess how it is affected by fix interval. We demonstrate how the maximum speed equation could potentially be used to derive a modified filter for telemetry data from highly mobile animals. Our results highlight the importance of incorporating fix‐interval information into comparisons of movement metrics from different herds and time periods. © 2013 The Wildlife Society.
Gender, Turning Points, and Boomerangs: Returning Home in Young Adulthood in Great Britain
Authors: Stone Juliet , Berrington Ann , Falkingham Jane 
Published: November 2013

AbstractThe idea of a generation of young adults “boomeranging” back to the parental home has gained widespread currency in the British popular press. However, there is little empirical research identifying either increasing rates of returning home or the factors associated with this trend. This article addresses this gap in the literature using data from a long-running household panel survey to examine the occurrence and determinants of returning to the parental home. We take advantage of the longitudinal design of the British Household Panel Survey (1991–2008) and situate returning home in the context of other life-course transitions. We demonstrate how turning points in an individual’s life course—such as leaving full-time education, unemployment, or partnership dissolution—are key determinants of returning home. An increasingly unpredictable labor market means that employment cannot be taken for granted following university graduation, and returning home upon completion of higher education is becoming normative. We also find that gender moderates the relationship among partnership dissolution, parenthood, and returning to the parental home, reflecting the differential welfare support in Great Britain for single parents compared with nonresident fathers and childless young adults.
Bayesian Peak Picking for NMR Spectra
Authors: Cheng Yichen , Gao Xin , Liang Faming 
Published: November 2013

Abstract
Protein structure determination is a very important topic in structural genomics, which helps people to understand varieties of biological functions such as protein-protein interactions, protein–DNA interactions and so on. Nowadays, nuclear magnetic resonance (NMR) has often been used to determine the three-dimensional structures of protein in vivo. This study aims to automate the peak picking step, the most important and tricky step in NMR structure determination. We propose to model the NMR spectrum by a mixture of bivariate Gaussian densities and use the stochastic approximation Monte Carlo algorithm as the computational tool to solve the problem. Under the Bayesian framework, the peak picking problem is casted as a variable selection problem. The proposed method can automatically distinguish true peaks from false ones without preprocessing the data. To the best of our knowledge, this is the first effort in the literature that tackles the peak picking problem for NMR spectrum data using Bayesian method.