bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of metachronous breast cancer after BRCA mutation–associated ovarian cancer
Authors: Domchek Susan M. , Jhaveri Komal , Patil Sujata , Stopfer Jill E. , Hudis Clifford , Powers Jacquelyn , Stadler Zsofia , Goldstein Laura , Kauff Noah , Khasraw Mustafa , Offit Kenneth , Nathanson Katherine L. , Robson Mark 
Published: November 2012

AbstractBACKGROUND:This study sought to estimate the risk of breast cancer (BC) after a diagnosis of ovarian cancer (OC) associated with mutation of the BRCA1/2 (breast cancer, early onset) genes (BRCA‐OC).METHODS:The Memorial Sloan‐Kettering Cancer Center and the University of Pennsylvania, clinical genetics databases were searched to identify women with BRCA‐OC who participated in genetic testing and follow‐up studies from 1995 to 2009. The primary objective was to determine the risk of developing BC after BRCA‐OC. Overall survival (OS) and BC‐free survival (BCFS) were determined by the Kaplan‐Meier method; patients were censored at the time of last follow‐up.RESULTS:A total of 164 patients had BRCA‐OC (115 with BRCA1; 49 with BRCA2). Of these 164 patients, 152 developed OC prior to BRCA testing (median time to testing, 2.4 years [0.01‐55 years]). Median follow‐up from OC for those not developing BC was 5.8 years (0.25‐55.6 years). There were 46 deaths, but none were due to BC. The 5‐ and 10‐year OS were 85% (95% confidence interval [CI] = 0.78, 0.90) and 68% (95% CI = 0.59, 0.76), respectively. There were 18 metachronous BC diagnoses. The 5‐ and 10‐year BCFS were 97% (95% CI = 0.92, 0.99) and 91% (95% CI = 0.82, 0.95), respectively. A subset of 64 women were tested either before or within 12 months of BRCA‐OC. In this pseudo‐incident subset, 5‐ and 10‐ year OS was 71% (95% CI = 0.53, 0.83) and 62% (95% CI = 0.44, 0.75), respectively, and 5‐ and 10‐year BCFS were 100% and 87% (95% CI = 0.56, 0.96), respectively.CONCLUSIONS:OS was dominated by OC deaths. Metachronous BC risk was lower than reported for unaffected BRCA mutation carriers. These results support nonsurgical management of BC risk in women with BRCA‐OC. Cancer 2013. © 2012 American Cancer Society.
Basal bodies exhibit polarized positioning in zebrafish cone photoreceptors
Authors: Ramsey Michelle , Perkins Brian D. 
Published: November 2012

AbstractThe asymmetric positioning of basal bodies, and therefore cilia, is often critical for proper cilia function. This planar polarity is critical for motile cilia function but has not been extensively investigated for nonmotile cilia or for sensory cilia such as vertebrate photoreceptors. Zebrafish photoreceptors form an organized mosaic ideal for investigating cilia positioning. We report that, in the adult retina, the basal bodies of red‐, green‐, and blue‐sensitive cone photoreceptors localized asymmetrically on the cell edge nearest the optic nerve. In contrast, no patterning was seen in the basal bodies of ultraviolet‐sensitive cones or in rod photoreceptors. The asymmetric localization of basal bodies was consistent in all regions of the adult retina. Basal body patterning was unaffected in the cones of the XOPS‐mCFP transgenic line, which lacks rod photoreceptors. Finally, the adult pattern was not seen in 7‐days‐postfertilization (dpf) larvae; basal bodies were randomly distributed in all the photoreceptor subtypes. These results establish the asymmetrical localization of basal bodies in red‐, green‐, and blue‐sensitive cones in adult zebrafish retinas but not in larvae. This pattern suggests an active cellular mechanism regulated the positioning of basal bodies after the transition to the adult mosaic and that rods do not seem to be necessary for the patterning of cone basal bodies. J. Comp. Neurol. 521:1803–1816, 2013. © 2012 Wiley Periodicals, Inc.
Designed Synthesis of Functionalized Two‐Dimensional Metal–Organic Frameworks with Preferential CO2 Capture
Authors: Yan Qiuju , Lin Yichao , Wu Pengyan , Zhao Li , Cao Lujie , Peng Luming , Kong Chunlong , Chen Liang 
Published: November 2012

AbstractIn metal–organic framework (MOF) chemistry, polar functionalities greatly affect the gas adsorption properties. However, synthesis of MOFs with desired functionality is very challenging because many chemical functionalities cannot be achieved under the conditions for MOF assembly. Herein, a facile synthesis of new functionalized two‐dimensional MOFs with preferential CO2 capture is presented, which uses two successive synthesis steps: 1) rational design and template‐free synthesis of the parent MOF with designated pendant amino groups and 2) postsynthetic modification of the active amino groups with acetic acid and trimesoyl chloride functionalities. The only variation in structure arises from the functional groups of these materials. Experimental results demonstrate that the three 2D layered MOFs have remarkable thermal stability and moisture resistance, which are particularly advantageous for practical CO2 capture. Although their surface areas are moderate (270–340 m2 g−1), they still have excellent CO2 adsorption capacity (up to 2.9 mmol g−1 at 1 bar and 273 K) comparable to that of previously reported MOFs with much higher surface areas. Based on first‐principles calculations, it is shown that the acidic carbonyl functionalities in addition to the amino groups are also favorable to bind CO2 molecules. The adsorption sites generated from polar functionalities are key factors leading to high CO2 uptake.
Toxicity evaluation of two typical surfactants to Dunaliella bardawil, an environmentally tolerant alga
Authors: Qv Xiao-Ying , Jiang Jian-Guo 
Published: November 2012

Abstract
Sodium dodecyl benzene sulfonate (SDBS) and cetyl trimethyl ammonium chloride (CTAC) are two kinds of surfactants widely applied in various industries. The tremendous direct discharge of these surfactants into natural waters has posed a significant threat to ecosystems. Dunaliella bardawil was employed in the present research to test the toxic effects of SDBS, CTAC, and their mixture on cell growth, cellular morphology, β-carotene accumulation, and enzymatic activities of superoxide dismutase (SOD) and catalase (CAT). The results showed that SDBS at 200, 550, 900, 1,350, 1,800, and 2,400 mg/L and CTAC at 0.4, 0.7, 1.0, 1.3, 2.8, and 3.5 mg/L inhibited algal growth and β-carotene accumulation, both of which declined and then increased. In particular, CTAC (median inhibitory concentration at 10 days [IC50]10 d = 2.8 ± 1.49 mg/L) was more hazardous than SDBS (IC5010 d = 2,044 ± 637.3 mg/L). The additive index (AI) calculated from carotene content data was (−4.10, −1.67) &lt; 0, indicating an antagonistic effect between SDBS and CTAC. Algae cultivated at level 6 of the binary system showed hormesis due to the mitigated toxicity; SDBS at 2,400 mg/L, CTAC at 3.5 mg/L, and combined surfactants at level 6 exerted lethal effects on D. bardawil. Both SOD and CAT activities showed similar associations with varied concentrations of surfactants: SOD was significantly promoted by 550 to 1,800 mg/L SDBS, 0.7 to 1.3 mg/L CTAC, and mixtures at levels 2 to 4; CAT was clearly promoted by 900 mg/L SDBS, 0.4 to 1.3 mg/L CTAC, and mixtures at levels 2 to 4. Environ. Toxicol. Chem. 2013;32:426–433. © 2012 SETAC
Effect of geometrical structure on the in vivo quality change of a three‐dimensionally perforated porous bone cell scaffold made of apatite/collagen composite
Authors: Otsuka Makoto , Nakagawa Hidenori , Otsuka Kuniko , Ito Atsuo , Higuchi William I. 
Published: November 2012

AbstractBiodegradable artificial bone blocks with interconnective pores were prepared using a self‐setting apatite/collagen composite cement as a cell scaffold for bone regenerative medicine. The biological behavior of the blocks was tested in rats, and the change in their properties after implantation was measured. One cubic block [10 mm X10 mm X10 mm; porous composite (PC)] was obtained from apatite cement (apatite/collagen cement; 80% of apatite:20% of collagen) with 60 interconnecting holes, 500 um in diameter. The other blocks (NC and NN) without holes were obtained from the apatite/collagen and plain apatite cements, respectively. All blocks were implanted in the rats for 56 days. Changes in the amount and density (block mineral mass and block mineral density) of the blocks were evaluated based on dual energy X‐ray absorptiometry images, and the order of biodegradation was PC < NC < NN. After implantation, the blocks were removed, and subjected to an X‐ray diffraction (XRD) analysis, Fourier‐transformed infrared (FT‐IR) spectroscopy and thermogravimetry (TG). The XRD peaks of all blocks increased significantly. TG revealed that the amount of carbonated apatite also increased with time. However, the organic component of PC depended on the implantation period, consistent with the FT‐IR results. Because PC had interconnective macro‐ and micropores in the apatite/collagen matrices, the results indicated that soft tissue penetrated the block carbonated apatite was generated, bone remodeling was accelerated in the implant. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Stiffness‐ and wettability‐dependent myoblast cell compatibility of transparent poly(vinyl alcohol) hydrogels
Authors: Gupta Siddhi , T Greeshma , Basu Bikramjit , Goswami Sudipta , Sinha Arvind 
Published: November 2012

AbstractThis study reports the in vitro compatibility of muscle cells (C2C12 mouse myoblast cell line) with the transparent poly(vinyl alcohol) (PVA) hydrogels and the results are explained on the basis of surface wettability, crystallinity, and nanoscale elastic stiffness property. Nanoindentation was carried out with a maximum load of 100 μN for all the hydrogel compositions and the properties such as elastic stiffness, hardness and total work done during indentation were computed. The difference in cell viability as well as adhesion of cultured myoblast cells on the investigated hydrogel substrates were discussed in reference to the difference in the nanoscale elastic properties, crystallinity, and surface wettability. An important result has been that both elastic stiffness and surface wettability synergistically influence myoblast viability/adhesion on PVA hydrogels. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2013.
Biophysical Study on the Interaction of Ceftriaxone Sodium with Bovine Serum Albumin Using Spectroscopic Methods
Authors: Pan Jiongwei , Ye Zaiting , Cai Xiaoping , Wang Liangxing , Cao Zhuo 
Published: November 2012

ABSTRACTThe interaction of ceftriaxone sodium (CS), a cephalosporin antibiotic, with the major transport protein, bovine serum albumin (BSA), was investigated using different spectroscopic techniques such as fluorescence, circular dichroism (CD), and UV–vis spectroscopy. Values of binding parameters for BSA–CS interaction in terms of binding constant and number of binding sides were found to be 9.00 × 103, 3.24 × 103, and 2.30 × 103 M−1 at 281, 301, and 321 K, respectively. Thermodynamic analysis of the binding data obtained at different temperatures showed that the binding process was spontaneous and was primarily mediated by van der Waals force or hydrogen bonding. CS binding to BSA caused secondary structural alterations in the protein as revealed by CD results. The distance between CS and Trp of BSA was determined as 3.23 nm according to the Förster resonance energy transfer theory. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:487‐492, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21446
Simultaneous removal of carbon, nitrogen and phosphorus in a multi‐zone wastewater treatment system
Authors: Alimahmoodi Mahmood , Yerushalmi Laleh , Mulligan Catherine N. 
Published: November 2012

AbstractBackgroundThe simultaneous removal of carbon, nitrogen and phosphorus under variable operating conditions during the start‐up period of a new multi‐environment hybrid wastewater treatment technology called BioCAST is reported in this paper. The presence of both suspended‐growth and immobilized microorganisms, as well as the continuous circulation of mixed liquor between the three zones of aerobic, microaerophilic and anoxic, supported high removal efficiencies of organic and inorganic contaminants.ResultsThe removal efficiencies of carbon, nitrogen and phosphorus reached 99.3%, 98.0% and 92.3%, respectively, under varying organic loading rates in the range 0.4–3.0 kg m‐3 d‐1, and nitrogen and phosphorus loading rates of 0.03–0.14 kg m‐3 d‐1 and 0.008–0.028 kg m‐3 d‐1, respectively. Phosphorus removal increased steadily with the increase of nitrogen loading rate, producing a final effluent concentration of 2.9 mg L‐1. Low sludge generation was obtained along with a low biomass yield of 11.5%.ConclusionsThe start‐up period of the BioCAST system demonstrated its potential for simultaneous removal of organic material, as well as nitrogen and phosphorus. Phosphorus removal demonstrated a strong dependence on nitrogen loading rate. Moreover, theoretical analysis of the liquid flow and energy balances in different zones of the treatment system showed that the pressure difference across the air diffuser is the governing force for the flow of liquid towards the aerobic zone and the controlling factor for the continuous circulation of mixed liquor among various zones. © 2012 Society of Chemical Industry
The disulfide bond of an RGD4C motif inserted within the Hi loop of the adenovirus type 5 fiber protein is critical for retargeting to αv‐integrins
Authors: Majhen Dragomira , Richardson Jennifer , Vukelić Bojana , Dodig Ivana , Cindrić Mario , Benihoud Karim , Ambriović‐Ristov Andreja 
Published: November 2012

AbstractBackgroundThe αv‐integrin binding motif RGD4C (CDCRGDCFC) has been used extensively to circumvent inefficient adenovirus type 5 (Ad5) transduction of cells expressing low levels of the coxsackie and adenovirus receptor. However, until now, it has been unclear whether disulfide bonds in the RGD4C motif influence the retargeting potential of RGD4C‐modified Ad5.MethodsReplication deficient Ad5 bearing wild‐type fiber (Ad5wt) or RGD4G, RGD4C and RGD2C2G insertions within the HI loop of the fiber protein (Ad5RGD4G, Ad5RGD4C and Ad5RGD2C2G, respectively) were used to transduce a panel of cancer cell lines, with or without previous treatment of these Ad5s with the reducing agent dithiothreitol (DTT). In parallel, native and DTT‐treated fiber proteins isolated from purified Ad5RGD4C were compared by mass spectrometry.ResultsAd5RGD4C transduced all studied cell lines much more efficiently than Ad5wt, whereas Ad5RGD4G transduced cells only slightly more efficiently than Ad5wt. DTT treatment had no effect on cell transduction by wild‐type Ad5wt and Ad5RGD4G but abolished the increased transduction efficacy of Ad5RGD4C in a dose‐dependent manner. The mass spectra of native and DTT‐reduced tryptic digests of the Ad5RGD4C fiber protein are consistent with the presence of a C547–C549 linkage in the C547DC549RGDC553FC555 motif. Finally, the high transduction efficacy of Ad5RGD4C is conserved in Ad5RGD2C2G.ConclusionsWe provide genetic and biochemical data strongly suggesting that cysteines C547 and C549 from the C547DC549RGDC553FC555 motif inserted in the HI loop of the Ad5 fiber form a single disulfide bond, with this disulfide bond being crucial for Ad5RGD4C retargeting to av‐integrins. Copyright © 2012 John Wiley & Sons, Ltd.
A stress MRI of the shoulder for evaluation of ligamentous stabilizers in acute and chronic acromioclavicular joint instabilities
Authors: Izadpanah Kaywan , Winterer Jan , Vicari Marco , Jaeger Martin , Maier Dirk , Eisebraun Leonie , Ute Will Jutta , Kotter Elmar , Langer Mathias , Südkamp Norbert P. , Hennig Jürgen , Weigel Mathias 
Published: November 2012

AbstractPurpose:To show the feasibility of a stress magnetic resonance imaging (MRI) as a new method for simultaneous evaluation of the morphology and the functional integrity of the acromioclavicular joint (ACJ) ligamentous stabilizers.Materials and Methods:MRI of four volunteers, 10 patients with acute, and six with chronic ACJ injuries was performed using a 0.25 T open MRI scanner. A 2D‐proton‐density and a 3D‐gradient‐echo sequence at rest and under 6.5 kg shoulder traction were performed. Comparative measurements of the coracoclavicular and the acromioclavicular distance were performed. Additionally, the conoid and trapezoid ligament lengths were measured with multiplanar reconstructions.Results:MRI at rest correctly identified tears of the coracoclavicular and the acromioclavicular ligaments in eight patients suffering acute ACJ injuries. Stress application helped to distinguish between partial and complete coracoclavicular ligament tears in two cases. Insufficiency of the ACJ ligaments was present in all acute and chronic ACJ injuries. Stress application in chronic ACJ ligaments revealed isolated insufficiency of the conoid ligament in three cases and of the trapezoid ligament in one case. Combined insufficiency was present in two cases.Conclusion:Stress MRI facilitates simultaneous acquisition of morphologic and functional information of the ACJ stabilizers. In acute ACJ injuries it helps to distinguish between partial and complete ligament tears. In chronic ACJ injuries it provides functional information of the ligament regrinds. J. Magn. Reson. Imaging 2013;37:1486–1492. © 2012 Wiley Periodicals, Inc.
Targeted biopsy based on ADC map in the detection and localization of prostate cancer: A feasibility study
Authors: Watanabe Yuji , Nagayama Masako , Araki Tohru , Terai Akito , Okumura Akira , Amoh Yoshiki , Ishimori Takayoshi , Nakashita Satoru , Dodo Yoshihiro 
Published: November 2012

AbstractPurpose:To investigate the feasibility of targeted biopsy based on an apparent diffusion coefficient (ADC) map in the detection and localization of prostate cancer.Materials and Methods:This study included 288 consecutive patients with high or increasing serum prostate‐specific antigen (PSA) levels who underwent prostatic magnetic resonance imaging (MRI) examination with an ADC map. Four core‐targeted biopsies of low ADC lesions were performed under transrectal‐ultrasound guidance with reference to ADC map. The positive predictive values (PPVs) of low ADC lesions were calculated and compared for the peripheral zone (PZ), transition zone (TZ), and anterior portion, respectively. Comparisons of ADC values and sizes between malignant and nonmalignant lesions were also performed.Results:A total of 313 low ADC lesions were detected in 195 patients and sampled by targeted biopsies. The PPVs were 55.3% (95% confidence interval [CI]: 50–61) in total, 61.0% (95% CI: 53–69) for PZ, 50.6% (95% CI: 43–58) for TZ, and 90.9% (95% CI: 81–100) for the anterior portion. The most common nonmalignant pathology of low ADC lesions was hyperplasia, followed by chronic prostatitis. There were significant differences in ADC values and sizes between malignant and nonmalignant low ADC lesions.Conclusion:Targeted biopsies could be capable of detecting cancers well wherever they may be in the prostate, although the PPVs varied depending on the location of low ADC lesions. J. Magn. Reson. Imaging 2013;37:1168–1177. © 2012 Wiley Periodicals, Inc.
A comparative assessment of monthly mean wind speed products over the global ocean
Authors: Kent Elizabeth C. , Fangohr Susanne , Berry David I. 
Published: November 2012

ABSTRACTThe accurate estimation of marine wind speed is important for climate and air–sea interaction applications. There are many datasets of monthly mean wind speeds available based either on in situ measurements, satellite retrievals, atmospheric reanalysis assimilating both in situ and satellite data and blended datasets combining some or all of these other data sources. Twelve different monthly mean wind speed datasets are compared for the period from 1987 to 2009. The results suggest that we cannot presently be confident that the mean wind speed over the ocean is known to the accuracy required for the calculation of air–sea heat fluxes. Comparisons are complicated by different representations of wind speed being presented in different datasets. The in situ and reanalysis datasets present stability‐dependent, earth‐relative, wind speeds adjusted to a reference level of 10 m. The satellite and blended datasets present neutral equivalent, surface‐relative, speeds adjusted to a reference level of 10 m. Differences between these estimates depend on atmospheric stability and ocean currents and can be greater than the required accuracy target. The adjustment for stability is itself uncertain but it is shown that these uncertainties are likely to be smaller than biases caused when the effects of stability are neglected. Further differences among the datasets are identified. Biases are caused by unidentified rain in Ku‐band scatterometer‐derived wind speeds and by atmospheric effects on passive microwave wind retrievals. When satellite observations affected by rain are removed a fair‐weather bias remains. Some datasets are biased low in coastal regions by the effects of lower wind speeds over land in atmospheric models affecting wind speeds near the coast. All these uncertainties combine to give a wide range of estimates of monthly mean wind speed for the chosen datasets with uncertainty in mean values, spatial patterns and changes over time.
Spatial coherence and potential predictability assessment of intraseasonal statistics of wet and dry spells over Equatorial Eastern Africa
Authors: Gitau Wilson , Ogallo Laban , Camberlin Pierre , Okoola Raphael 
Published: November 2012

ABSTRACTThe aim of this study was to derive components of the intraseasonal rainfall variations from the daily rainfall in the Equatorial Eastern Africa region and assess their spatial coherence, a pointer to their potential predictability. Daily rainfall observations from 36 stations distributed over Equatorial Eastern Africa and extending from 1962 to 2000 were used. The March to May and October to December periods commonly referred to as the long and short rainfall seasons respectively were considered.Seasonal and intraseasonal statistics at the local (station) level were first defined. The stations were also grouped into near‐homogeneous (sub‐regional) zones based on daily rainfall. Similarly, seasonal and intraseasonal statistics were then derived at sub‐regional level using three different approaches. Inter‐station correlation coefficients of the intraseasonal statistics at local levels were finally computed and plotted as box‐plots.For the two rainfall seasons, the two statistics showing the highest spatial coherence were the seasonal rainfall totals and the number of the wet days at sub‐regional level. The local variance explained for these two variables, as an average over all the sub‐regions, was more than 40%. At the bottom of the hierarchy were the mean rainfall intensity and frequency of dry spells of 5 days or more which showed the least coherence, with the local variance explained being less than 10% in each season. For each of the intraseasonal components of daily rainfall considered, the short rainfall season statistics were more coherent compared to the long rainfall season. Lag‐correlations with key indices depicting sea‐surface temperatures in the Pacific and Indian Oceans showed that the hierarchy between the rainfall statistics in the strength of the teleconnections reflected that of spatial coherence.
Effect of thermal and non‐thermal pasteurisation on the microbial inactivation and phenolic degradation in fruit juice: a mini‐review
Authors: Chen Yougui , Yu Li Juan , Rupasinghe HP Vasantha 
Published: November 2012

AbstractFruit juice has been traditionally preserved by thermal pasteurisation. However, the applied heat can cause detrimental effects on health‐promoting components such as phenolic compounds. Several non‐thermal technologies such as membrane filtration, pulsed electric field (PEF) and ultraviolet (UV) exposure are promising methods developed for liquid food preservation. In particular, the combination of UV and PEF has proven to be more effective for microbial inactivation and maintaining nutritional quality of fruit juice compared with individual applications. © 2012 Society of Chemical Industry