bluuebunny/crossref_metadata_2025_split huggingface dataset:
A quantitative comparison of dispersed spore/pollen and plant megafossil assemblages from a Middle Jurassic plant bed from Yorkshire, UK
Authors: Slater Sam M. , Wellman Charles H. 
Published: October 2015

AbstractDetailed quantitative data has previously been collected from plant megafossil assemblages from a Middle Jurassic (Aalenian) plant bed from Hasty Bank, North Yorkshire, UK. We conducted a similar analysis of palynological dispersed sporomorph (spore and pollen) assemblages collected from the same section using the same sampling regime: 67 sporomorph taxa were recorded from 50 samples taken at 10 cm intervals through the plant bed. Basic palynofacies analysis was also undertaken on each sample. Both dispersed sporomorph and plant megafossil assemblages display consistent changes in composition, diversity (richness), and abundance through time. However, the dispersed sporomorph and plant megafossil records provide conflicting evidence for the nature of parent vegetation. Specifically, conifers and ferns are underrepresented in plant megafossil assemblages, bryophytes and lycopsids are represented only in sporomorph assemblages, and sphenophytes, pteridosperms, Caytoniales, Cycadales, Ginkgoales and Bennettitales are comparatively underrepresented in sporomorph assemblages. Combined multivariate analysis (correspondence analysis and nonmetric multidimensional scaling) of sporomorph occurrence/abundance data demonstrates that temporal variation in sporomorph assemblages is the result of depositional change through the plant bed. The reproductive strategies of parent plants are considered to be a principal factor in shaping many of the major abundance and diversity irregularities between dispersed sporomorph and plant megafossil data sets that seemingly reflects different parent vegetation. Preferential occurrence/preservation of sporomorphs and equivalent parent plants is a consequence of a complex array of biological, ecological, geographical, taphonomic, and depositional factors that act inconsistently between and within fossil assemblages, which results in notable discrepancies between data sets.
ResearchingMahrin Germany: A Multidisciplinary Approach
Authors: Günther Ursula , Herzog Martin , Müssig Stephanie 
Published: October 2015

AbstractThis article considers the legal institution ofmahrin Islamic family law from three research perspectives in order to provide insights into the phenomenon's complexity, particularly with regard to current legal practices. In particular, emphasis is placed both on countries where family law is shaped by Islamic traditions (e.g., Morocco) and on countries whose legal traditions do not have a mahr counterpart (e.g., Germany). First, the social and economic function of dower will be described. As a special form of property transfer, mahr will be analyzed in its historical and present shape in theory and practice. Second, the legal conceptualization of mahr in the German legal context will be discussed. The example of Morocco serves to illustrate the changes with regard to mahr because of the process of incorporation of Islamic legal concepts into a national statutory law system. Given the Muslim diaspora, these insights are important contributions to the legal intepretation of mahr in a transnational context.
MARTYROLOGY AND CONCEPTIONS OF TIME IN HIZBULLAH'S WRITING PRACTICES
Authors: Saade Bashir 
Published: October 2015

AbstractSoon after its founding in the early 1980s, the Lebanese political organization Hizbullah developed a specific practice of remembering its dead. In this article, I argue that through this practice Hizbullah constructed an elaborate conception of time and history that gave ideological coherence to the movement's main political project,al-muqāwama al-islāmiyya(Islamic Resistance). Examining early writings in the Hizbullah weeklyal-ʿAhdpublished during the organization's formative period, I show how such writings were instrumental in producing ideological templates that have continued to be replicated until today. Through a set of ritualistic practices, Hizbullah-affiliated intellectuals have archived everything related to martyrs and other kinds of human legacies, a process that has fed into the notion of an ever-present, and at times anticipated, era (ʿahd) of resistance. Moreover, the project of Islamic Resistance has gained salience each time the past is relived in the present, producing political action. Hizbullah's efforts at history writing have involved a transmission of ethics through martyrs' act of witnessing and their testimony to a way of life. Analyzing this phenomenon sheds light on the way political Islamic groups such as Hizbullah articulate national imaginaries through specific kinds of ideological production.
Agronomic traits, ensilability and nutritive value of five pearl millet cultivars grown in a Brazilian semi-arid region
Authors: SANTOS R. D. DOS , NEVES A. L. A. , PEREIRA L. G. R. , SOLLENBERGER L. E. , RODRIGUES J. A. S. , TABOSA J. N. , VERNEQUE R. S. , OLIVEIRA G. F. , JAYME D. G. , GONÇALVES L. C. 
Published: October 2015

SUMMARYPearl millet (Pennisetum glaucum(L.) R.) could play an important role as a feed source for ruminants in arid and semi-arid zones of the world owing to its high yield and drought tolerance. The current paper assessed the agronomic characteristics, ensilability, intake and digestibility of five Brazilian pearl millet cultivars (IPA Bulk1BF, BRS 1501, CMS-03, CMS-01 and BN-2) in a typical Brazilian northeastern semi-arid climate. Forage was harvested at the dough stage of grain maturity (growth stage 86 according to the BBCH scale) and ensiled under laboratory and farm conditions. Apparent digestibility of the silages was determined using 25 Santa Inês male lambs. The cultivars CMS-01, CMS-03 and BN-2 out-performed the others in terms of dry matter (DM) and digestible DM yield/ha. At DM partitioning among plant tissues, the cultivar IPA Bulk1BF had a greater DM associated with panicles and one of the greatest concentrations of organic matter, lactic acid andin vitrodry matter digestibility among the five cultivars. The cultivar BRS 1501 had greater butyric acid concentration as well as one of the highest pH values. Silage produced from BN-2 not only contained greater acetic acid concentration, but also showed one of the greatest total volatile fatty acid concentrations. There were no differences in feed intake and digestibility of nutrients and fibre fractions across all cultivars. Silage made from BN-2 resulted in greater urinary excretion of nitrogen than those produced from BRS 1501. Under the conditions of the present study, the results obtained for production of DM and digestible dry matter, and the ratio of plant fractions, indicates the possible use of these cultivars for silage production in the Brazilian semi-arid region.
A novel coagulation inhibitor fromSchistosoma japonicum
Authors: RANASINGHE SHIWANTHI L. , FISCHER KATJA , GOBERT GEOFFREY N. , MCMANUS DONALD P. 
Published: October 2015

SUMMARYLittle is known about the molecular mechanisms whereby the human blood flukeSchistosoma japonicumis able to survive in the host venous blood system. Protease inhibitors are likely released by the parasite enabling it to avoid attack by host proteolytic enzymes and coagulation factors. Interrogation of theS. japonicumgenomic sequence identified a gene,SjKI-1, homologous to that encoding a single domain Kunitz protein (Sjp_0020270) which we expressed in recombinant form inEscherichia coliand purified.SjKI-1is highly transcribed in adult worms and eggs but its expression was very low in cercariae and schistosomula.In situimmunolocalization with anti-SjKI-1 rabbit antibodies showed the protein was present in eggs trapped in the infected mouse intestinal wall. In functional assays, SjKI-1 inhibited trypsin in the picomolar range and chymotrypsin, neutrophil elastase, FXa and plasma kallikrein in the nanomolar range. Furthermore, SjKI-1, at a concentration of 7·5µm, prolonged 2-fold activated partial thromboplastin time of human blood coagulation. We also demonstrate that SjKI-1 has the ability to bind Ca++. We present, therefore, characterization of the first Kunitz protein fromS. japonicumwhich we show has an anti-coagulant properties. In addition, its inhibition of neutrophil elastase indicates SjKI-1 have an anti-inflammatory role. Having anti-thrombotic properties, SjKI-1 may point the way towards novel treatment for hemostatic disorders.
Defending the Authoritarian Regime Online: China's “Voluntary Fifty-cent Army”
Authors: Han Rongbin 
Published: October 2015

AbstractRecent studies on internet politics in China have gone beyond the once dominant control–liberalization perspective and directed intellectual attention to the varieties of online activism. Based on extensive in-depth online ethnographic work, this project explores the pluralization of online expression in Chinese cyberspace. Following a constituency of internet users who identify themselves as the “voluntary fifty-cent army,” the paper explores how these users acquire and consolidate their identity and combat criticism that targets the authoritarian regime. Analysis of the confrontational exchanges between the “voluntary fifty-cent army” and their opponents suggests that a perspective that goes beyond state censorship and regime-challenging activism is required in order to gain a better understanding of online expression in China. Close examination of why and how internet users may voluntarily defend the authoritarian regime also reveals how the dynamics in online discourse competition may work to the authoritarian regime's advantage.
Environmental factor analysis of cholera in China using remote sensing and geographical information systems
Authors: XU M. , CAO C. X. , WANG D. C. , KAN B. , XU Y. F. , NI X. L. , ZHU Z. C. 
Published: October 2015

SUMMARYCholera is one of a number of infectious diseases that appears to be influenced by climate, geography and other natural environments. This study analysed the environmental factors of the spatial distribution of cholera in China. It shows that temperature, precipitation, elevation, and distance to the coastline have significant impact on the distribution of cholera. It also reveals the oceanic environmental factors associated with cholera in Zhejiang, which is a coastal province of China, using both remote sensing (RS) and geographical information systems (GIS). The analysis has validated the correlation between indirect satellite measurements of sea surface temperature (SST), sea surface height (SSH) and ocean chlorophyll concentration (OCC) and the local number of cholera cases based on 8-year monthly data from 2001 to 2008. The results show the number of cholera cases has been strongly affected by the variables of SST, SSH and OCC. Utilizing this information, a cholera prediction model has been established based on the oceanic and climatic environmental factors. The model indicates that RS and GIS have great potential for designing an early warning system for cholera.
Accurate lineshape spectroscopy and the Boltzmann constant
Authors: Truong G.-W. , Anstie J. D. , May E. F. , Stace T. M. , Luiten A. N. 
Published: October 2015

AbstractSpectroscopy has an illustrious history delivering serendipitous discoveries and providing a stringent testbed for new physical predictions, including applications from trace materials detection, to understanding the atmospheres of stars and planets, and even constraining cosmological models. Reaching fundamental-noise limits permits optimal extraction of spectroscopic information from an absorption measurement. Here, we demonstrate a quantum-limited spectrometer that delivers high-precision measurements of the absorption lineshape. These measurements yield a very accurate measurement of the excited-state (6P1/2) hyperfine splitting in Cs, and reveals a breakdown in the well-known Voigt spectral profile. We develop a theoretical model that accounts for this breakdown, explaining the observations to within the shot-noise limit. Our model enables us to infer the thermal velocity dispersion of the Cs vapour with an uncertainty of 35 p.p.m. within an hour. This allows us to determine a value for Boltzmann’s constant with a precision of 6 p.p.m., and an uncertainty of 71 p.p.m.
Stability of single skyrmionic bits
Authors: Hagemeister J. , Romming N. , von Bergmann K. , Vedmedenko E. Y. , Wiesendanger R. 
Published: October 2015

AbstractThe switching between topologically distinct skyrmionic and ferromagnetic states has been proposed as a bit operation for information storage. While long lifetimes of the bits are required for data storage devices, the lifetimes of skyrmions have not been addressed so far. Here we show by means of atomistic Monte Carlo simulations that the field-dependent mean lifetimes of the skyrmionic and ferromagnetic states have a high asymmetry with respect to the critical magnetic field, at which these lifetimes are identical. According to our calculations, the main reason for the enhanced stability of skyrmions is a different field dependence of skyrmionic and ferromagnetic activation energies and a lower attempt frequency of skyrmions rather than the height of energy barriers. We use this knowledge to propose a procedure for the determination of effective material parameters and the quantification of the Monte Carlo timescale from the comparison of theoretical and experimental data.
ATP binding to neighbouring subunits and intersubunit allosteric coupling underlie proteasomal ATPase function
Authors: Kim Young-Chan , Snoberger Aaron , Schupp Jane , Smith David M. 
Published: October 2015

AbstractThe primary functions of the proteasome are driven by a highly allosteric ATPase complex. ATP binding to only two subunits in this hexameric complex triggers substrate binding, ATPase–20S association and 20S gate opening. However, it is unclear how ATP binding and hydrolysis spatially and temporally coordinates these allosteric effects to drive substrate translocation into the 20S. Here, we use FRET to show that the proteasomal ATPases from eukaryotes (RPTs) and archaea (PAN) bind ATP with high affinity at neighbouring subunits, which complements the well-established spiral-staircase topology of the 26S ATPases. We further show that two conserved arginine fingers in PAN located at the subunit interface work together as a single allosteric unit to mediate the allosteric effects of ATP binding, without altering the nucleotide-binding pattern. Rapid kinetics analysis also shows that ring resetting of a sequential hydrolysis mechanism can be explained by thermodynamic equilibrium binding of ATP. These data support a model whereby these two functionally distinct allosteric networks cooperate to translocate polypeptides into the 20S for degradation.
Topological data analysis for discovery in preclinical spinal cord injury and traumatic brain injury
Authors: Nielson Jessica L. , Paquette Jesse , Liu Aiwen W. , Guandique Cristian F. , Tovar C. Amy , Inoue Tomoo , Irvine Karen-Amanda , Gensel John C. , Kloke Jennifer , Petrossian Tanya C. , Lum Pek Y. , Carlsson Gunnar E. , Manley Geoffrey T. , Young Wise , Beattie Michael S. , Bresnahan Jacqueline C. , Ferguson Adam R. 
Published: October 2015

AbstractData-driven discovery in complex neurological disorders has potential to extract meaningful syndromic knowledge from large, heterogeneous data sets to enhance potential for precision medicine. Here we describe the application of topological data analysis (TDA) for data-driven discovery in preclinical traumatic brain injury (TBI) and spinal cord injury (SCI) data sets mined from the Visualized Syndromic Information and Outcomes for Neurotrauma-SCI (VISION-SCI) repository. Through direct visualization of inter-related histopathological, functional and health outcomes, TDA detected novel patterns across the syndromic network, uncovering interactions between SCI and co-occurring TBI, as well as detrimental drug effects in unpublished multicentre preclinical drug trial data in SCI. TDA also revealed that perioperative hypertension predicted long-term recovery better than any tested drug after thoracic SCI in rats. TDA-based data-driven discovery has great potential application for decision-support for basic research and clinical problems such as outcome assessment, neurocritical care, treatment planning and rapid, precision-diagnosis.
Three-dimensional super-resolution protein localization correlated with vitrified cellular context
Authors: Liu Bei , Xue Yanhong , Zhao Wei , Chen Yan , Fan Chunyan , Gu Lusheng , Zhang Yongdeng , Zhang Xiang , Sun Lei , Huang Xiaojun , Ding Wei , Sun Fei , Ji Wei , Xu Tao 
Published: October 2015

AbstractWe demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more photons under our cryogenic imaging condition, resulting in higher localization precision which is comparable to ambient super-resolution imaging. Vitrified specimens were prepared by high pressure freezing and cryo-sectioning to maintain a near-native state with better fluorescence preservation. A 2-3-fold improvement of resolution over the recent reports was achieved due to the photon budget performance of screening out Dronpa and optimized imaging conditions, even with thin sections which is at a disadvantage when calculate the structure resolution from label density. We extended csCLEM to mammalian cells by introducing cryo-sectioning and observed good correlation of a mitochondrial protein with the mitochondrial outer membrane at nanometer resolution in three dimensions.
The Hepatitis E virus intraviral interactome
Authors: Osterman Andreas , Stellberger Thorsten , Gebhardt Anna , Kurz Marisa , Friedel Caroline C. , Uetz Peter , Nitschko Hans , Baiker Armin , Vizoso-Pinto Maria G. 
Published: October 2015

AbstractHepatitis E virus (HEV) is an emerging virus causing epidemic acute hepatitis in developing countries as well as sporadic cases in industrialized countries. The life cycle of HEV is still poorly understood and the lack of efficient cell culture systems and animal models are the principal limitations for a detailed study of the viral replication cycle. Here we exhaustively examine all possible intraviral protein-protein interactions (PPIs) of HEV by systematic Yeast two-hybrid (Y2H) and LuMPIS screens, providing a basis for studying the function of these proteins in the viral replication cycle. Key PPIs correlate with the already published HEV 3D structure. Furthermore, we report 20 novel PPIs including the homodimerization of the RNA dependent RNA polymerase (RdRp), the self-interaction of the papain like protease and ORF3 interactions with the papain-like protease and putative replicase components: RdRp, methylase and helicase. Furthermore, we determined the dissociation constant (Kd) of ORF3 interactions with the viral helicase, papain-like protease and methylase, which suggest a regulatory function for ORF3 in orchestrating the formation of the replicase complex. These interactions may represent new targets for antiviral drugs.
Neurotoxic Methamphetamine Doses Increase LINE-1 Expression in the Neurogenic Zones of the Adult Rat Brain
Authors: Moszczynska Anna , Flack Amanda , Qiu Ping , Muotri Alysson R. , Killinger Bryan A. 
Published: October 2015

AbstractMethamphetamine (METH) is a widely abused psychostimulant with the potential to cause neurotoxicity in the striatum and hippocampus. Several epigenetic changes have been described after administration of METH; however, there are no data regarding the effects of METH on the activity of transposable elements in the adult brain. The present study demonstrates that systemic administration of neurotoxic METH doses increases the activity of Long INterspersed Element (LINE-1) in two neurogenic niches in the adult rat brain in a promoter hypomethylation-independent manner. Our study also demonstrates that neurotoxic METH triggers persistent decreases in LINE-1 expression and increases the LINE-1 levels within genomic DNA in the striatum and dentate gyrus of the hippocampus and that METH triggers LINE-1 retrotransposition in vitro. We also present indirect evidence for the involvement of glutamate (GLU) in LINE-1 activation. The results suggest that LINE-1 activation might occur in neurogenic areas in human METH users and might contribute to METH abuse-induced hippocampus-dependent memory deficits and impaired performance on several cognitive tasks mediated by the striatum.
E2 superfamily of ubiquitin-conjugating enzymes: constitutively active or activated through phosphorylation in the catalytic cleft
Authors: Valimberti Ilaria , Tiberti Matteo , Lambrughi Matteo , Sarcevic Boris , Papaleo Elena 
Published: October 2015

AbstractProtein phosphorylation is a modification that offers a dynamic and reversible mechanism to regulate the majority of cellular processes. Numerous diseases are associated with aberrant regulation of phosphorylation-induced switches. Phosphorylation is emerging as a mechanism to modulate ubiquitination by regulating key enzymes in this pathway. The molecular mechanisms underpinning how phosphorylation regulates ubiquitinating enzymes, however, are elusive. Here, we show the high conservation of a functional site in E2 ubiquitin-conjugating enzymes. In catalytically active E2s, this site contains aspartate or a phosphorylatable serine and we refer to it as the conserved E2 serine/aspartate (CES/D) site. Molecular simulations of substrate-bound and -unbound forms of wild type, mutant and phosphorylated E2s, provide atomistic insight into the role of the CES/D residue for optimal E2 activity. Both the size and charge of the side group at the site play a central role in aligning the substrate lysine toward E2 catalytic cysteine to control ubiquitination efficiency. The CES/D site contributes to the fingerprint of the E2 superfamily. We propose that E2 enzymes can be divided into constitutively active or regulated families. E2s characterized by an aspartate at the CES/D site signify constitutively active E2s, whereas those containing a serine can be regulated by phosphorylation.
Mitochondrial respiratory dysfunction due to the conversion of substituted cathinones to methylbenzamides in SH-SY5Y cells
Authors: den Hollander Bjørnar , Sundström Mira , Pelander Anna , Siltanen Antti , Ojanperä Ilkka , Mervaala Eero , Korpi Esa R. , Kankuri Esko 
Published: October 2015

AbstractThe increased use of cathinone-type designer drugs, known as legal highs, has led to concerns about their potential neurotoxicity due to their similarity to methamphetamine (METH). Therefore, closer investigations of their toxic effects are needed. We investigated the effects of the cathinones 4-methylmethcathinone (4-MMC) and 3,4-methylenedioxymethcathinone (MDMC) and the amphetamine METH on cytotoxicity and mitochondrial respiration in SH-SY5Y neuroblastoma cells. We also investigated the contribution of reactive species, dopamine, Bcl-2 and tumor necrosis factor α (TNFα) on toxicity. Finally, we investigated the effect of cathinone breakdown products using ultra-high performance liquid chromatography/high-resolution time-of-flight mass spectrometry and studied their involvement in toxicity. We observed dose-dependent increases in cytotoxicity and decreases in mitochondrial respiration following treatment with all cathinones and amphetamines. Glutathione depletion increases amphetamine, but not cathinone toxicity. Bcl-2 and TNFα pathways are involved in toxicity but dopamine levels are not. We also show that cathinones, but not amphetamines, spontaneously produce reactive species and cytotoxic methylbenzamide breakdown products when in aqueous solution. These results provide an important first insight into the mechanisms of cathinone cytotoxicity and pave the way for further studies on cathinone toxicityin vivo.