bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum biomarkers reflecting specific tumor tissue remodeling processes are valuable diagnostic tools for lung cancer
Authors: Willumsen Nicholas , Bager Cecilie L. , Leeming Diana J. , Smith Victoria , Christiansen Claus , Karsdal Morten A. , Dornan David , Bay‐Jensen Anne‐Christine 
Published: July 2014

AbstractExtracellular matrix (ECM) proteins, such as collagen type I and elastin, and intermediate filament (IMF) proteins, such as vimentin are modified and dysregulated as part of the malignant changes leading to disruption of tissue homeostasis. Noninvasive biomarkers that reflect such changes may have a great potential for cancer. Levels of matrix metalloproteinase (MMP) generated fragments of type I collagen (C1M), of elastin (ELM), and of citrullinated vimentin (VICM) were measured in serum from patients with lung cancer (n = 40), gastrointestinal cancer (n = 25), prostate cancer (n = 14), malignant melanoma (n = 7), chronic obstructive pulmonary disease (COPD) (n = 13), and idiopathic pulmonary fibrosis (IPF) (n = 10), as well as in age‐matched controls (n = 33). The area under the receiver operating characteristics (AUROC) was calculated and a diagnostic decision tree generated from specific cutoff values. C1M and VICM were significantly elevated in lung cancer patients as compared with healthy controls (AUROC = 0.98, P < 0.0001) and other cancers (AUROC = 0.83 P < 0.0001). A trend was detected when comparing lung cancer with COPD+IPF. No difference could be seen for ELM. Interestingly, C1M and VICM were able to identify patients with lung cancer with a positive predictive value of 0.9 and an odds ratio of 40 (95% CI = 8.7–186, P < 0.0001). Biomarkers specifically reflecting degradation of collagen type I and citrullinated vimentin are applicable for lung cancer patients. Our data indicate that biomarkers reflecting ECM and IMF protein dysregulation are highly applicable in the lung cancer setting. We speculate that these markers may aid in diagnosing and characterizing patients with lung cancer.
Thoracic aortic aneurysm expansion due to late distal stent graft‐induced new entry
Authors: Jánosi Rolf Alexander , Tsagakis Konstantinos , Bettin Markus , Kahlert Philipp , Horacek Michael , Al‐Rashid Fadi , Schlosser Thomas , Jakob Heinz , Eggebrecht Holger , Erbel Raimund 
Published: July 2014

ObjectivesThis study analyzed the mechanism and risk factors of thoracic aortic aneurysm expansion due to late distal stent graft‐induced new entry (dSINE).BackgroundThis late complication of thoracic endovascular aneurysm repair (TEVAR) for aortic dissection is under‐recognized but potentially life‐threatening.MethodsIn 142 patients who underwent TEVAR with endovascular entry sealing for acute and chronic aortic type B dissection, using commercially available straight (nontapered) stent‐grafts, we examined the oversizing rate, the aortic taper ratio, and the need for reintervention.ResultsNine of 142 patients developed thoracic aortic aneurysm expansion due to dSINE after TEVAR. The median follow‐up was 47.5 ± 37.4 months. There was a significant difference in the distal stent–aorta angle between the patients with and without dSINE (149.08 ± 15.09° vs. 166.72 ± 12.47°, P < 0.005). Patients with dSINE showed a significantly higher taper ratio of the true lumen of the aorta (40.9 ± 14.13% vs. 25.36 ± 20.2%, P < 0.05). There was also a significant difference in the oversizing of the stent‐graft in the distal landing zone (95.88 ± 49.3% vs. 55.94 ± 36.23%, P < 0.01). All patients with dSINE underwent a secondary endograft procedure without any complications or deaths. In 7 cases we used a custom‐made, highly tapered stent‐graft.ConclusionsLifelong follow‐up of patients is mandatory after TEVAR. A stent‐graft with a tapered design should be used in aortic dissection to avoid oversizing and devastating late complications. © 2014 Wiley Periodicals, Inc.
Effect of Various Au/Al2O3 Preparations on Catalytic Behaviour during the Continuous Flow Hydrogenation of an Octanal/Octene Mixture
Authors: Chetty Thashini , Friedrich Holger B. , Dasireddy Venkata D. B. C. , Govender Alisa , Mohlala Pheladi J. , Barnard Werner 
Published: July 2014

AbstractSupported gold catalysts were prepared on a γ‐Al2O3 support by using the incipient wet impregnation and the deposition–precipitation methods. These catalysts were assessed for the continuous flow, liquid phase hydrogenation of an octanal/octene mixture, and their activity was compared with the activity of a commercially available Au/Al2O3 catalyst. These catalysts were characterised by inductively coupled plasma optical emission spectroscopy, hydrogen chemisorption, BET surface area and pore volume measurements, Raman spectroscopy, temperature‐programmed reduction of hydrogen, energy‐dispersive X‐ray spectroscopy mapping, HRTEM and powder XRD to determine gold crystallites. The results obtained revealed that gold crystallite size, dispersion and the presence of gold chloride had a significant effect on the product selectivity obtained. Large gold crystallites (particle size >15 nm) unevenly distributed on the alumina support obtained by using the wet impregnation method were inactive in hydrogenation. Hydrogen spillover resulted in the formation of protonic acid sites originating from molecular hydrogen, together with the formation of HCl‐catalysed acetal, and yielded 90 mol % selectivity towards the C24 acetal. In contrast, small gold crystallites (particle size <10 nm) evenly distributed on the alumina support obtained by using the deposition–precipitation preparation method facilitated the hydrogenation of the aldehyde and yielded 75 mol % selectivity towards the desired product, octanol.
Identification and Application of Enantiocomplementary Lactamases for Vince Lactam Derivatives
Authors: Assaf Zeinab , Eger Elisabeth , Vitnik Zeljko , Fabian Walter M. F. , Ribitsch Doris , Guebitz Georg M. , Faber Kurt , Hall Mélanie 
Published: July 2014

AbstractFour enzymes showing hydrolytic activity on derivatives of 2‐azabicyclo[2.2.1]hept‐5‐en‐3‐one (Vince lactam) were successfully identified through analysis of protein crystal structure and amino acid sequence alignments. Enantiocomplementary activities were observed on Vince lactam and its saturated analog 2‐azabicyclo[2.2.1]heptan‐3‐one with non‐heme chloroperoxidase (CPO‐T) from Streptomyces aureofaciens, cyclic imide hydrolase (CIH) from Pseudomonas putida, polyamidase (NfpolyA) from Nocardia farcinica, and amidase (AMI) from Rhodococcus globerulus, and perfect kinetic resolution was achieved (E>200). Computational analysis of amide bond resonance stabilization in lactams correlated well with the overall reactivity pattern of the lactams as a function of ring size and strain. The biocatalysts cloned and investigated in this study could be of interest for the synthesis of enantiopure carbocyclic nucleoside analogues.
σ–π Conjugated Organosilicon Hybrid Polymers from Copolymerization of a Tetrasiladiene and 1,4‐Diethynylbenzene
Authors: Majumdar Moumita , Bejan Iulia , Huch Volker , White Andrew J. P. , Whittell George R. , Schäfer André , Manners Ian , Scheschkewitz David 
Published: July 2014

AbstractA catalyst‐ and by‐product‐free protocol for the synthesis of σ–π conjugated organosilicon polymers is reported. The regiospecific [2+2] cycloaddition of CC triple bonds to SiSi double bonds allowed the preparation of air‐stable ethynyl‐terminated extended monomers from 1,4‐bis(ethynyl)benzene and the para‐phenylene bridged tetrasiladiene, Tip2SiSiTip‐pC6H4‐SiTipSiTip2 (Tip=2,4,6‐iPr3C6H2). The polymer obtained from the extended monomer and further tetrasiladiene exhibits pronounced σ–π conjugation, as was evident from the red‐shift in the absorption spectrum compared to model systems. We show that the thermal stability of the employed bis(alkyne) co‐monomer is translated into this polymer.
Bis‐Pyrene‐Modified Unlocked Nucleic Acids: Synthesis, Hybridization Studies, and Fluorescent Properties
Authors: Perlíková Pavla , Ejlersen Maria , Langkjær Niels , Wengel Jesper 
Published: July 2014

AbstractEfficient synthesis of a building block for the incorporation of a bis‐pyrene‐modified unlocked nucleic acid (UNA) into oligonucleotides (DNA*) was developed. The presence of bis‐pyrene‐modified UNA within a duplex leads to duplex destabilization that is more profound in DNA*/RNA and less distinct in DNA*/DNA duplexes. Nevertheless, the destabilization effect of bis‐pyrene‐modified UNA is weaker than that of unmodified UNA. Some oligonucleotides with bis‐pyrene‐modified UNA incorporations displayed superior mismatch discrimination capabilities. UV/Vis absorption and molecular modeling studies indicate that the pyrene groups of bis‐pyrene‐modified UNA are located in the major groove of a duplex. Oligonucleotides containing two bis‐pyrene‐modified UNA monomers showed low pyrene monomer emission in bulge‐containing duplexes, high pyrene monomer emission in fully matched duplexes, and 5‐(pyrenyl)uracil:pyrene exciplex emission in the single‐stranded form. Such fluorescent properties enable the application of bis‐pyrene‐modified UNA in the development of fluorescence probes for DNA/RNA detection and for detection of deletions at specific positions.
The Heat Equation Shrinks Ising Droplets to Points
Authors: Lacoin Hubert , Simenhaus François , Toninelli Fabio 
Published: July 2014

Let be a bounded, smooth enough domain of ℝ2. For L > 0 consider the continuous‐time, zero‐temperature heat bath stochastic dynamics for the nearest‐neighbor Ising model on (ℤ/L)2 (the square lattice with lattice spacing 1/L) with initial condition such that σx =−1 if x ∊  and σx = + 1 otherwise. We prove the following classical conjecture due to H. Spohn: In the diffusive limit where time is rescaled by L2 and L → ∞, the boundary of the droplet of “‐” spins follows a deterministic anisotropic curve‐shortening flow such that the normal velocity is given by the local curvature times an explicit function of the local slope. Locally, in a suitable reference frame, the evolution of the droplet boundary follows the one‐dimensional heat equation.To our knowledge, this is the first proof of mean‐curvature‐type droplet shrinking for a lattice model with genuine microscopic dynamics.An important ingredient is in our forthcoming work, where the case of convex was solved. The other crucial point in the proof is obtaining precise regularity estimates on the deterministic curve‐shortening flow. This builds on geometric and analytic ideas of Grayson, Gage and Hamilton, Gage and Li, Chou and Zhu, and others.© 2015 Wiley Periodicals, Inc.
Towards Rational Modulation of In‐Plane Molecular Arrangements in Metal–Organic Framework Nanosheets
Authors: Makiura Rie , Usui Ryo , Sakai Yuta , Nomoto Akihiro , Ogawa Akiya , Sakata Osami , Fujiwara Akihiko 
Published: July 2014

AbstractA strategy to modulate the in‐plane structural arrangement in preferentially oriented crystalline metal–organic framework (MOF) nanosheets assembled by a two‐dimensional interfacial reaction between porphyrin units and metal ion linkers is reported. Starting with a tetratopic porphyrin MOF nanofilm, NAFS‐2, the framework size and shape are modified by employing specially designed building units, a trans‐ditopic and an expanded tetratopic porphyrin, and Cu2+ linkers. Reducing the number of binding parts affords a MOF nanosheet, NAFS‐31, with a distorted in‐plane structure. Extension of the peripheral substituents, while maintaining the tetratopic porphyrin geometry, results in marked unit cell size enlargement in an undistorted square grid in the MOF nanofilm, NAFS‐41. The exquisite geometric control that these structural modifications entail is valuable to allow switching of chemical/physical properties of the nanosheets and lead to realization of their use in nanotechnological applications.
Cadmium Sulfide Quantum Dots Supported on Gallium and Indium Oxide for Visible‐Light‐Driven Hydrogen Evolution from Water
Authors: Pan Yun‐xiang , Zhuang Huaqiang , Hong Jindui , Fang Zheng , Liu Hai , Liu Bin , Huang Yizhong , Xu Rong 
Published: July 2014

AbstractIn this work, CdS quantum dots (QDs) supported on Ga2O3 and In2O3 are applied for visible‐light‐driven H2 evolution from aqueous solutions that contain lactic acid. With Pt as the cocatalyst, the H2 evolution rates on CdS/Pt/Ga2O3 and CdS/Pt/In2O3 are as high as 995.8 and 1032.2 μmol h−1, respectively, under visible light (λ>420 nm) with apparent quantum efficiencies of 43.6 and 45.3 % obtained at 460 nm, respectively. These are much higher than those on Pt/CdS (108.09 μmol h−1), Pt/Ga2O3 (0.12 μmol h−1), and Pt/In2O3 (0.05 μmol h−1). The photocatalysts have been characterized thoroughly and their band structures and photocurrent responses have been measured. The band alignment between the CdS QDs and In2O3 can lead to interfacial charge separation, which cannot occur between the CdS QDs and Ga2O3. Among the various possible factors that contribute to the high H2 evolution rates on CdS/Pt/oxide, the surface properties of the metal oxides play important roles, which include (i) the anchoring of CdS QDs and Pt nanoparticles for favorable interactions and (ii) the efficient trapping of photogenerated electrons from the CdS QDs because of surface defects (such as oxygen defects) based on photoluminescence and photocurrent studies.
Vitamin B1‐Catalyzed Acetoin Formation from Acetaldehyde: A Key Step for Upgrading Bioethanol to Bulk C4 Chemicals
Authors: Lu Ting , Li Xiukai , Gu Liuqun , Zhang Yugen 
Published: July 2014

AbstractThe production of bulk chemicals and fuels from renewable biobased feedstocks is of significant importance for the sustainability of human society. The production of ethanol from biomass has dramatically increased and bioethanol also holds considerable potential as a versatile building block for the chemical industry. Herein, we report a highly selective process for the conversion of ethanol to C4 bulk chemicals, such as 2,3‐butanediol and butene, via a vitamin B1 (thiamine)‐derived N‐heterocyclic carbene (NHC)‐catalyzed acetoin condensation as the key step to assemble two C2 acetaldehydes into a C4 product. The environmentally benign and cheap natural catalyst vitamin B1 demonstrates high selectivity (99 %), high efficiency (97 % yield), and high tolerance toward ethanol and water impurities in the acetoin reaction. The results enable a novel and efficient process for ethanol upgrading.
NetFCM: A semi‐automated web‐based method for flow cytometry data analysis
Authors: Frederiksen Juliet , Buggert Marcus , Karlsson Annika C. , Lund Ole 
Published: July 2014

AbstractMulti‐parametric flow cytometry (FCM) represents an invaluable instrument to conduct single cell analysis and has significantly increased our understanding of the immune system. However, due to new techniques allowing us to measure an increased number of phenotypes within the immune system, FCM data analysis has become more complex and labor‐intensive than previously. We have therefore developed a semi‐automatic gating strategy (NetFCM) that uses clustering and principal component analysis (PCA) together with other statistical methods to mimic manual gating approaches. NetFCM is an online tool both for subset identification as well as for quantification of differences between samples. Additionally, NetFCM can classify and cluster samples based on multidimensional data. We tested the method using a data set of peripheral blood mononuclear cells collected from 23 HIV‐infected individuals, which were stimulated with overlapping HIV Gag‐p55 and CMV‐pp65 peptides or medium alone (negative control). NetFCM clustered the virus‐specific CD8+ T cells based on IFNγ and TNF responses into distinct compartments. Additionally, NetFCM was capable of identifying HIV‐ and CMV‐specific responses corresponding to those obtained by manual gating strategies. These data demonstrate that NetFCM has the potential to identify relevant T cell populations by mimicking classical FCM data analysis and reduce the subjectivity and amount of time associated with such analysis. © 2014 International Society for Advancement of Cytometry
Diiron Azamonothiolates by the Scission of Dithiadiazacyclooctanes by Iron Carbonyls
Authors: Lin Tai , Ulloa Olbelina A. , Rauchfuss Thomas B. , Gray Danielle L. 
Published: July 2014

AbstractThe reactions of [Fe3(CO)12] with the dithiadiazacyclooctanes [SCH2N(R)CH2]2 (R = Me, Bn) afford the diiron complexes [Fe2{SCH2N(R)CH2}(CO)6] (1Me, 1Bu). The methyl derivative 1Me was characterized crystallographically [Fe–Fe = 2.5702(5) Å]. Its low symmetry was verified by variable‐temperature 13C NMR spectroscopy, which revealed that the turnstile rotation of the {S(CH2)Fe(CO)3} and {S(NMe)Fe(CO)3} centers are subject to very different energy barriers. Although 1Me resists protonation, it readily undergoes substitution by tertiary phosphines, first at the {S(CH2)Fe(CO)3} center, as verified crystallographically for [Fe2{SCH2N(Me)CH2}(CO)5(PPh3)]. Substitution by the chelating diphosphine Ph2PCH2CH2PPh2 (dppe) gave [Fe2{SCH2N(Me)CH2}(CO)4(dppe)] by substitution at both the {S(CH2)Fe(CO)3} and {S(NMe)Fe(CO)3} sites.
Salicylate‐Functionalized Bismuth Oxido Clusters: Hydrolysis Processes and Microbiological Activity
Authors: Schlesinger Maik , Pathak Amita , Richter Sebastian , Sattler Dominik , Seifert Andreas , Rüffer Tobias , Andrews Philip C. , Schalley Christoph A. , Lang Heinrich , Mehring Michael 
Published: July 2014

AbstractHydrolysis of either Bi(HSal4Me)3 (A) or [Bi22O26(HSal4Me)14](B) in dmso gave the 4‐methylsalicylate‐substituted bismuth oxido cluster [Bi38O45(HSal4Me)24(dmso)14(H2O)2]·4H2O (1·4H2O), which crystallizes in the triclinic space group P$\bar {1}$ with cell parameters of a = 20.7214(5), b = 20.9654(6), c = 22.2128(6) Å, α = 100.867(2), β = 114.108(2), γ = 107.895(2)° and V = 7815.1(4) Å3. The hydrolysis of A was studied by using 1H DOSY NMR spectroscopy and electrospray ionization Fourier‐transform ion‐cyclotron‐resonance (ESI‐FTICR) mass spectrometry, both of which showed the formation of several larger species under ambient conditions in the presence of moisture. Furthermore, ESI‐FTICR MS analysis of cluster B showed the formation of mainly {Bi23O26} species, which indicates the partial dissociation of cluster B in solution. In addition, the three different bismuth species containing 1 (A), 22 (B) or 38 (1·4H2O) bismuth atoms per molecular formula were tested for their microbiological activity against three strains of Helicobacter pylori (251, 26695 and B128). A minimum inhibitory concentration (MIC) of 6.25 μg mL–1 for the mononuclear bismuth complex A was obtained, whereas the bismuth oxido cluster B showed a lower activity with MIC values in the range 25–50.0 μg mL–1. The activity of 1·4H2O is comparable to commercial remedies based on antimicrobial bismuth subsalicylate (BSS; 12.5 μg mL–1).
Roseic Acid and Roseolactones A and B, Furan‐Cucurbitane Triterpenes from Russula aurora and R. minutula (Basidiomycota)
Authors: Clericuzio Marco , Vidari Giovanni , Cassino Claudio , Legnani Laura , Toma Lucio 
Published: July 2014

AbstractThree new seco‐cucurbitane triterpenes, viz. roseic acid (1) and roseolactones A (2) and B (3), all bearing a furan moiety condensed to the B‐ring, were isolated from Russula aurora and R. minutula. Their structures were elucidated using 1D and 2D NMR spectroscopy, and ESI HRMS. Compounds 2 and 3 were found to be epimers at C‐23. A detailed ab initio theoretical investigation was carried out to assess their relative stereochemistries. The conformations and NMR spectra of the two isomers were calculated, and the results were compared to the experimental data. The new compounds show a bright pink reaction with sulfo‐vanillin at room temperature, and they are therefore responsible for the systematically significant reaction performed by mycologists on R. aurora and R. minutula fruiting bodies. A mixture of compounds 2 and 3 was found to be weakly active against HepG2, WISH, and CAKI‐1 human tumour cells.
Graphene Paste Electrode: Analytical Applications for the Quantification of Dopamine, Phenolic Compounds and Ethanol
Authors: Gutierrez Fabiana , Comba Fausto N. , Gasnier Aurélien , Gutierrez Alejandro , Galicia Laura , Parrado Concepción , Rubianes María D. , Rivas Gustavo A. 
Published: July 2014

AbstractThis work reports the analytical applications of a graphene paste electrode (GrPE) for the quantification of dopamine, ethanol and phenolic compounds. Dopamine was detected by differential pulse voltammetry‐adsorptive stripping with medium exchange at submicromolar levels even in the presence of high excess of ascorbic acid and serotonin. The electrocatalytic activity of graphene towards the oxidation of NADH and the reduction of quinones allowed the sensitive amperometric determination of ethanol and phenols using GrPE modified with alcohol dehydrogenase/NAD+ or polyphenol oxidase, respectively, with successful applications in real samples like alcoholic beverages and tea.
Probabilistic seismic response assessment of linear systems equipped with nonlinear viscous dampers
Authors: Tubaldi Enrico , Ragni Laura , Dall'Asta Andrea 
Published: July 2014

SUMMARYThe paper aims at evaluating the influence of damper properties on the probabilistic seismic response of structural systems equipped with nonlinear viscous dampers.For this purpose, a linear single‐degree‐of‐freedom system with an added linear or nonlinear viscous damper is considered, and the response statistics are evaluated for a set of natural records describing the ground motion uncertainty. A dimensional analysis of the seismic problem is carried out first to identify the minimum set of characteristic parameters describing the system and controlling the seismic response. An extensive parametric study is then performed to estimate the influence of the damper properties on the statistics of the main response quantities of interest (i.e. maximum displacements, accelerations and damper forces), for a wide range of values of the characteristic parameters. Finally, a set of case studies is investigated to show some interesting issues concerning the influence of the damper nonlinear behaviour on the evaluation of the system reliability and to highlight some limitations of current deterministic approaches neglecting the probabilistic properties of the response. Copyright © 2014 John Wiley & Sons, Ltd.