bluuebunny/crossref_metadata_2025_split huggingface dataset:
Canine Intra‐Articular Multipotent Stromal Cells (MSC) From Adipose Tissue Have the Highest In Vitro Expansion Rates, Multipotentiality, and MSC Immunophenotypes
Authors: Zhang Nan , Dietrich Marilyn A. , Lopez Mandi J. 
Published: February 2013

AbstractObjectiveTo identify the optimum intra‐articular multipotent stromal cell (MSC) tissue source in the canine stifle.Study DesignExperimental.Sample PopulationInfrapatellar adipose tissue, synovium lining the joint capsule, and synovium surrounding the cranial cruciate ligament (CrCL) from normal stifles of 6 dogs.MethodsNucleated cell density for each tissue was determined, and cell doublings (CD) and doubling times (DT) were quantified for expansion rates. Adipogenic, osteogenic, and chondrogenic differentiation was confirmed with light microscopy. Fibroblastic, adipogenic, and osteogenic colony forming unit frequencies were determined for multipotentiality. Tissue‐specific target gene expression was assessed, and percentages of CD29+, CD34+, CD44+, CD45+, and CD90+ cells quantified.ResultsAdipose tissue had the highest MSC density (ASC). The CD decreased with increasing passages for all cell types, and ASC values tended to be higher. Multipotentiality decreased with passage, but remained highest in ASC. Tissue‐specific target gene expression was higher in induced versus noninduced cells, and ASCs had the highest upregulation across passages. Most cells were CD29+, CD44+, CD90+, and percentages decreased with passage. Within cell types, there were more CD29+ ASC in early passages and more CD44+ and CD90+ ASC in later passages.ConclusionsASC had the highest in vitro expansion rates, CFU frequencies, tissue‐specific target gene expression, and percentages of MSC immunophenotypes.
In Vitro Acute Load to Failure and Eyelet Abrasion Testing of a Novel Veterinary Screw‐Type Mini‐Anchor Design
Authors: Kunkel Kevin A. R. , Rusly Roy J. , Basinger R. Randy , DesJardins John D. , Gerard Patrick D. 
Published: February 2013

AbstractObjectiveTo determine acute load to failure (ALF) and suture abrasion (SA) at 0° and 90° for a novel screw‐type mini‐anchor design.Study DesignBiomechanical in vitro study.Sample PopulationSynthetic bone.MethodsTwenty mini‐anchors were inserted into synthetic bone blocks assigned to 1 of 2 groups (0° ALF, 90° ALF). Pullout was performed at 5 mm/min. ALF, yield strength and stiffness were calculated. SA constructs were created with 4 groups of 5 anchors each with either 30 lb nylon leader line (NLL), 40 lb NLL, #2 Fiberwire or #5 Fiberwire. SA was performed at 0° and 90° with a sinusoidal wave form at 0.5 Hz and 10 N load for 1000 cycles or until failure. Data were summarized as mean ± SD. ALF data were analyzed using t‐tests. SA data were analyzed using log rank, Tukey‐adjusted pairwise comparisons and sign tests. Significance was set at P = .05.ResultsMean ± SD ALF at 0° and 90° was 431.8 ± 70.8 N and 683 ± 48.7 N, respectively. 90° ALF was significantly higher. Yield strength and stiffness were not significantly different at 0° and 90°. #5 and #2 Fiberwire survived significantly more cycles than 40 lb and 30 lb NLL at 90°. At 0°, 30 lb NLL survived significantly less cycles than either Fiberwire size. Suture orientation did not have a significant effect on SA for Fiberwire constructs.ConclusionThe novel mini‐anchor has ALF comparable to other mini‐anchors. Fiberwire survived more cycles in the novel anchor eyelet than NLL and FW suture orientation in the eyelet did not affect SA.
Laparoscopic‐Assisted Placement of a Peritoneal Dialysis Catheter With Partial Omentectomy and Omentopexy in Dogs: An Experimental Study
Authors: Dupré Gilles , Čoudek Karel 
Published: February 2013

AbstractObjectiveTo describe a technique of laparoscopic‐assisted placement of a peritoneal dialysis (PD) catheter with simultaneously performed partial omentectomy and omentopexy.Study DesignPilot experimental study.AnimalsBeagle dogs (n = 6).MethodsAfter placement of 1 sub‐umbilical laparoscope portal and 1 instrument portal in the left cranial abdominal quadrant, laparoscopic‐assisted partial omentectomy, and omentopexy were performed, and a modified Tenckhoff PD catheter was placed under laparoscopic guidance. A modified dialysis protocol was used twice daily for 4 days. The feasibility of the procedure, surgical duration, operative complications, and dialysis efficacy were evaluated. Postoperative pain and inflammation were graded (0–3).ResultsThe procedure was successfully performed in all dogs with a median operating time of 25 minutes. No operative complications occurred. Procedure‐related postoperative pain and inflammation were minimal. Eight consecutive PD procedures were successfully performed, and no leakage or obstruction was observed.ConclusionsLaparoscopic‐assisted partial omentectomy and omentopexy can be performed at the same time as PD catheter placement with minimal morbidity.
Diagnostic Accuracy of Tissue Impedance Measurement Interpretation for Correct Veress Needle Placement in Feline Cadavers
Authors: Hyink Sara , Whittemore Jacqueline C. , Mitchell Amanda , Reed Ann 
Published: February 2013

AbstractObjectiveTo determine the diagnostic accuracy of tissue impedance measurement interpretation (TIMI) for determining correct versus incorrect Veress needle placement in feline cadavers.Study DesignProspective, randomized, blinded trial.Study PopulationCat cadavers (n = 24).MethodsTwo laparoscopists (1 experienced, 1 novice), blinded to TIMI, placed reusable Veress needles in study subjects in a randomized order. A third individual interpreted impedance measurements as consistent with correct versus incorrect placement. Veress needle tip locations were marked by injecting contrasting colors of India ink. Tissue dissection was performed to localize ink. Sensitivity, specificity, accuracy, precision, and kappa statistics for TIMI for placements by the experienced and novice laparoscopist were determined. P < .05 was considered significant.ResultsTIMI identified 36/38 correct and 2/10 incorrect placements. TIMI identified 2/2 bowel perforations but was unable to identify 8 inappropriate placements in the retroperitoneal fat pad. Impedance measurement interpretation had 94.7% sensitivity, 20% specificity, 79.2% accuracy, and 81% precision overall. Agreement between TIMI and Veress needle location was absent (kappa = −0.15, P = .01) for placements by the experienced laparoscopist and substantial (kappa = 0.78, P < .01) for the novice laparoscopist.ConclusionsFailure of TIMI to identify placement in the retroperitoneal fat pad resulted in poor accuracy. Small cat size limited the number of appropriate placement sites, perhaps resulting in excessively dorsal placements. Use of TIMI may increase detection of clinically significant inappropriate Veress needle placements, like bowel perforations, and decrease installment phase complications. Further evaluation of Veress needle placement with and without TIMI is warranted.
Muscle and Tendon Heating Rates with Therapeutic Ultrasound in Horses
Authors: Montgomery Leslie , Elliott Sarah B. , Adair H. Steve 
Published: February 2013

AbstractObjectiveTo (1) determine the temperature change in equine tendon and muscle during therapeutic ultrasound and (2) develop guidelines for treating horses for muscular or tendinous injury using therapeutic ultrasound.Study DesignExperimental, in vivo study.AnimalsAdult horses (n = 10).MethodsThermistors were inserted in the superficial and deep digital flexor tendons (SDFT and DDFT) of the thoracic limbs of 10 adult horses. On the left, 3.3 MHz therapeutic continuous ultrasound was done for 10 minutes at an intensity of 1.0 W/cm2 and for the right thoracic limb at 1.5 W/cm2. Thermistors were placed at 1 cm, 4 cm, and 8 cm depths in the epaxial muscles of the same 10 horses, for a 20‐minute treatment at a frequency of 3.3 MHz and intensity of 1.5 W/cm2. Temperature was recorded before, during, and after treatment. Data were statistically analyzed.ResultsMean temperature rise was 3.5°C in the SDFT and 2.5°C in the DDFT at the end of the 1.0 W/cm2 treatment (P = .94) and 5.2°C in the SDFT and 3.0°C in the DDFT at the end of the 1.5‐W/cm2 treatment (P = .48). Mean temperature rise in epaxial musculature was 1.3°C at a depth of 1.0 cm, 0.7°C at 4.0 cm, and 0.7°C at 8 cm.ConclusionsThe SDFT and DDFT are heated to a therapeutic temperature using a frequency of 3.3 MHz and intensity of 1.0 W/cm2. The epaxial muscles are not heated to a therapeutic temperature using a frequency of 3.3 MHz and an intensity of 1.5 W/cm2.
Arthroscopically Accessible Anatomy of the Tarsal Collateral Ligaments in the Horse
Authors: Kümmerle Jan M. , Kummer Martin R. 
Published: February 2013

AbstractObjectiveTo describe the arthroscopically accessible anatomy of the tarsal collateral ligaments in the horse.Study DesignDescriptive study.AnimalsCadaveric equine hind limbs (n = 24) obtained from horses without tarsal disease.MethodsTwo pairs of tarsal joints were used to obtain silicone models of the tarsocrural joint and dissect the tarsal collateral ligaments (CLs). Ten pairs of tarsocrural joints had arthroscopic exploration and the accessible parts of the tarsal CLs were marked with an arthroscopic hook knife. Subsequently, the limbs were dissected and the marked positions were allocated to the specific parts of the CLs.ResultsIpsilateral arthroscope and instrument portals were used in each pouch of the tarsocrural joint. Via the plantaromedial pouch, the superficial (2/20 limbs), middle (7), deep (6) or both middle and deep (3) short medial CL and the long medial CL (14) were reached. Access via the plantarolateral pouch allowed manipulation of the deep short lateral CL (20/20 limbs) and the long lateral CL (20). Dorsally, arthroscopy via the dorsomedial pouch gave access to the deep short medial CL (20/20 limbs), while the superficial (9) or middle (10) or both superficial and middle (1) short lateral CL could be reached via the dorsolateral joint recess.ConclusionsLimited parts of the tarsal CLs can be observed or manipulated arthroscopically in normal equine tarsocrural joints.
A Multiple‐Baseline Study of the Effects Associated With Metacognitive Therapy in Postpartum Depression
Authors: Bevan Denise , Wittkowski Anja , Wells Adrian 
Published: February 2013

Introduction: Postpartum depression is a disabling psychological disorder that, if left untreated, may have negative consequences for the mother, her partner, and the child. Although women with postpartum depression often experience symptoms of anxiety as well as depression, this has been underresearched. As metacognitive therapy assumes that the underlying mechanisms for both anxiety and depression are the same, it is a potentially useful psychological treatment for postpartum depression.Methods: A pilot study was carried out using a multiple‐baseline single case series to examine the effects associated with metacognitive therapy in the treatment of depression. Six women with postpartum depression were assigned to no‐treatment baselines of 3 to 6 weeks, followed by 8 to 12 sessions of metacognitive therapy. Follow‐up with participants took place at 3 and 6 months posttreatment.Results: All participants experienced clinically significant reductions in symptoms of depression and anxiety, with corresponding reductions in metacognitive beliefs consistent with the idea that this may be the mechanism by which change occurred. Furthermore, all scores fell within the normal range posttreatment, and effect sizes were large. Treatment gains were maintained at 3 and 6 months posttreatment by all participants.Discussion: This pilot study suggests that metacognitive therapy may be an effective psychological treatment for postpartum depression.
Do Hookups Hurt? Exploring College Students’ Experiences and Perceptions
Authors: Bachtel Molly Kathleen 
Published: February 2013

Introduction: Hookups refer to a range of sexual or physically intimate behaviors characterized by a lack of any expectation of a relationship or emotional intimacy. This study was designed to assess college students’ experiences and perceptions about hookups as related to mental/emotional and physical health issues.Methods: Students at a 2‐year liberal arts college in the southeastern United States participated. A survey was created using current evidence on hookups and health issues. Random sampling invited 300 students to take the anonymous online survey. Responses to open‐ended questions were analyzed quantitatively; content analysis was used to assess responses to 2 open‐ended questions.Results: Participants (N = 210) reported their own or their friends’ experiences as a result of a hookup. Feelings included sexual regret (reported by 62%), relationship/interpersonal conflict (48%), negative emotional reactions (47%), negative social repercussions (41%), learning to “shut down” emotionally (33%), psychological distress (31%), loss of friend(s) or peer group (28%), anxiety (28%), depression (21%), and sexually transmitted infections (19%). Most students (60%) had at least one hookup experience; more than half (55%) had mixed feelings/ambivalence about these experiences. Qualitative analysis identified 6 themes regarding reasons for sexual regret: regret over choice of partner, negative social repercussions, hopes for relationship did not materialize, moral issues, too much/too fast/too drunk, and suboptimal sexual performance/experience.Discussion: The findings indicate that a significant level of negative health issues is experienced in conjunction with hookups, and health care providers should be aware and educate patients appropriately.
End‐of‐life care in a rehabilitation centre for older people in Australia
Authors: Tan Heather , Digby Robin , Bloomer Melissa , Wang Yixin , O'Connor Margaret 
Published: February 2013

AimTo investigate processes at the end of life for patients who died in a subacute evaluation and management facility for older people.MethodsA retrospective chart audit for patients (n = 55) who died in the previous 2 years was undertaken, recording a number of significant variables.ResultsDespite diagnosis of comorbid medical conditions, most participants were admitted for improved functioning or assessment for alternative accommodation. Consistent with this focus, the key contact person was most often an allied health team member. Not For Resuscitation order and/or power of attorney documents on admission were uncommon (<30%) as were referrals to palliative care specialist staff (13%), although an end‐of‐life discussion was recorded (90%) and often included as a new goal of care (71%).ConclusionFactors likely to improve end‐of‐life care include advance care planning, earlier recognition of short prognosis and staff education.
Weight gain in steer and heifer calves treated with zeranol or oestradiol 17β
Authors: SAWYER G J 
Published: February 2013

SUMMARY: Male and female Hereford and Hereford cross beef calves between 2.5 and 4.5 months of age were implanted with 36 mg zeranol, 24 mg oestradiol 17β or left untreated, and then weighed every 4 to 5 weeks. Calves treated with zeranol or oestradiol 17β gained 9.6 kg (8.2%) and 12.9 kg (11.0%) more weight respectively than untreated calves. Steer calves gained more weight than heifer calves. There was no response to either growth promotant in heifers or in zeranol‐treated steers beyond 35 days after implantation, and the only significant further gains in the next 31 days were made by steers treated with oestradiol 17β. After this time growth of treated and control calves was similar. The lack of a continued response to the growth promotants was not associated with significantly lower growth rates in any weighing interval.Possible reasons for the duration of response observed and the difference in response between the sexes are discussed.
Rhinosporidiosis in the parotid duct: a rare case report
Authors: Panda Swagatika , Lenka Sthitaprajna , Padhiary Subrat K. , Sahoo Sujit R. , Srivastava Gunjan 
Published: February 2013

AbstractEndemic to South India and Sri Lanka, Rhinosporidiosis is a chronic granulomatous infection caused by an agent of uncertain taxonomy: Rhinosporidium seeberi. Although it commonly manifests as a proliferative nasal lesion, many cases of Rhinosporidiosis have been reported where it has appeared as an extranasal lesion. The reported extranasal sites include the eye, ear, trachea, and parotid duct. However, the involvement of the parotid duct is quite rare, even among extranasal sites. The case presented is an adult female from the non‐endemic zone of East India with a proliferative mass in the parotid duct. Although Rhinosporidiosis was not taken into consideration in the clinical differential diagnosis, eventual histopathological diagnosis confirmed Rhinosporidiosis. As this appears to be the second case of Rhinosporidiosis in the parotid duct in East India in 4 years, we encourage clinicians to be flexible in the differential diagnosis of proliferative growth in the parotid duct, even in those from non‐endemic areas.
Marine shelf habitat: biogeography and evolution
Authors: Briggs John C. , Bowen Brian W. 
Published: February 2013

AbstractWe synthesize the evolutionary implications of recent advances in the fields of phylogeography, biogeography and palaeogeography for shallow‐water marine species, focusing on marine speciation and the relationships among the biogeographic regions and provinces of the world. A recent revision of biogeographic provinces has resulted in the recognition of several new provinces and a re‐evaluation of provincial relationships. These changes, and the information that led to them, make possible a clarification of distributional dynamics and evolutionary consequences. Most of the new conclusions pertain to biodiversity hotspots in the tropical Atlantic, tropical Indo‐West Pacific, cold‐temperate North Pacific, and the cold Southern Ocean. The emphasis is on the fish fauna, although comparative information on invertebrates is utilized when possible. Although marine biogeographic provinces are characterized by endemism and thus demonstrate evolutionary innovation, dominant species appear to arise within smaller centres of high species diversity and maximum interspecies competition. Species continually disperse from such centres of origin and are readily accommodated in less diverse areas. Thus, the diversity centres increase or maintain species diversity within their areas of influence, and are part of a global system responsible for the maintenance of biodiversity over much of the marine world.
Efficacy/Safety of a Fixed‐Dose Amlodipine/Olmesartan Medoxomil–Based Treatment Regimen in Hypertensive Blacks and Non‐Blacks With Uncontrolled BP on Prior Antihypertensive Monotherapy
Authors: Nesbitt Shawna , Shojaee Ali , Maa Jen‐Fue 
Published: February 2013

In this secondary analysis of a dose‐titration study of patients with hypertension uncontrolled on prior monotherapy, blacks (n=234) and non‐blacks (n=765) were switched to amlodipine (AML)/olmesartan medoxomil (OM) 5/20 mg, with uptitration every 4 weeks to AML/OM 5/40 mg and then AML/OM 10/40 mg to achieve a seated cuff blood pressure (SeBP) of <120/70 mm Hg. Hydrochlorothiazide 12.5 and 25 mg could be added if SeBP was ≥125/75 mm Hg. The cumulative proportions of patients achieving systolic SeBP <140 mm Hg (<130 mm Hg if diabetic) at 12 weeks were 71.6% for blacks and 77.2% for non‐blacks. Mean SeBP change from baseline in blacks (mean baseline BP: 153.0/93.7 mm Hg) ranged from −11.7/−6.1 mm Hg for AML/OM 5/20 mg to −23.6/−12.9 mm Hg for AML/OM 10/40 mg +hydrochlorothiazide 25 mg (all P<.0001). Antihypertensive efficacy was maintained throughout the 24‐hour dosing interval. An AML/OM‐based regimen was effective in blacks with hypertension uncontrolled on prior monotherapy.
Effects of Add‐On Nebivolol on Blood Pressure and Glucose Parameters in Hypertensive Patients With Prediabetes
Authors: Deedwania Prakash , Shea John , Chen Wei , Brener Lillian 
Published: February 2013

In this multicenter trial, the effects of nebivolol added to an angiotensin‐converting enzyme (ACE) inhibitor or angiotensin II receptor blocker (ARB) were assessed in patients with hypertension (diastolic blood pressure [DBP] 80–110 mm Hg) and prediabetes (fasting blood glucose 100–125 mg/dL and/or 2‐hour oral glucose tolerance test [OGTT] 140–199 mg/dL). After a 4‐week run‐in period (in which lisinopril [10 mg/d] or losartan [50 mg/d] treatment was initiated), patients with DBP 90–110 mm Hg were randomized (2:2:1) to 12‐week, double‐blind treatment with nebivolol (n=223; 5–40 mg/d), hydrochlorothiazide (HCTZ; n=212; 12.5–25 mg/d), or placebo (n=102), titrated to achievement of 130/80 mm Hg. The primary outcome measure was DBP (last observation carried forward, intent to treat population); secondary measures included systolic blood pressure (SBP) and glucose levels. At baseline, overall mean values for body mass index, triglycerides, and high‐density lipoprotein cholesterol were 32.3 kg/m2, 1.7 mmol/L, and 1.3 mmol/L, respectively. At week 12, nebivolol and placebo groups demonstrated a decrease of −9.4 and −5.0 mm Hg, respectively (P<.001) for DBP and −10.4 and −7.8 mm Hg for SBP (P=.147). The mean changes in area under the curve OGTT were 0.0 mg/dL (nebivolol), 6.9 mg/dL (HCTZ; P=.024 vs nebivolol), and −1.0 mg/dL (placebo). Adverse event–related discontinuation rates were 10.3%, 6.6%, and 2.0%, respectively. Nebivolol, added to an ACE inhibitor or ARB, provides additional blood pressure reduction with little or no effect on glucose metabolism in hypertensive patients with prediabetes.
Epidermolysis bullosa acquisita
Authors: Kim J.H. , Kim S.‐C. 
Published: February 2013

AbstractEpidermolysis bullosa acquisita (EBA) is a chronic autoimmune subepidermal bullous disease with clinical features similar to the genetic form of dystrophic epidermolysis bullosa. EBA is characterized by the presence of autoantibodies against type VII collagen which is a major component of the anchoring fibrils at the dermal‐epidermal junction. EBA can be divided into two main clinical types; mechanobullous and inflammatory EBA. Mechanobullous EBA, referred to as classic EBA, presents with skin fragility, blisters and dystrophic changes on trauma‐prone areas. Inflammatory EBA resembles other autoimmune subepidermal bullous diseases. Compelling evidence from mouse models supports a pathogenic role of autoantibodies against type VII collagen in EBA. Treatment of EBA is often unsatisfactory. The most widely used systemic treatment is corticosteroids. Colchicine and dapsone have been reported to be good treatment modalities when combined with corticosteroids. Some intractable cases of EBA have successfully been treated with intravenous immunoglobulin or rituximab.