bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental pollutant 3-methyl-4-nitrophenol promotes the expression of oncostatin M to exacerbate airway allergic inflammation
Authors: Mo Lihua , Wang Xinxin , Liao Yun , Liu Yu , Tang Aifa , Li Jing , Yang Pingchang 
Published: August 2024

Abstract
Asthma exacerbation is a common clinical occurrence. The causal factors are not fully understood yet. Environmental pollution is linked to asthma exacerbation. The objective of this study is to elucidate the role of 3-methyl-4-nitrophenol (MNP), an environmental pollutant, in asthma exacerbation. In this study, an airway allergy mouse model was established with ovalbumin as a specific antigen with or without the presence of MNP. The results showed that, in a mouse model, the intensity of airway allergy was significantly increased by exposure to MNP. RNAseq results showed an increase in endoplasmic reticulum (ER) stress-associated molecules and the Osm expression in airway epithelial cells of mice with airway allergy. Exposure of epithelial cells to MNP in culture induced the expression of oncostatin M (OSM) and ER stress associated molecules. The OSM receptor was expressed by macrophages. OSM could drive macrophages to produce tumor necrosis factor-α (TNF-α). Inhibition of PERK, one of the key molecules of ER stress, or depletion of OSM receptor in macrophages, could effectively attenuate the MNP/ovalbumin protocol induced airway allergy. To sum up, by promoting ER stress, environmental pollutant MNP can cause airway epithelial cells to produce OSM. The latter induces macrophages to produce TNF-α, which can exacerbate airway allergy.
A real-world analysis of adherence, biochemical outcomes, and healthcare costs in patients treated with rosuvastatin/ezetimibe as single-pill combination vs. free combination in Italy
Authors: Zambon Alberto , Liberopoulos Evangelos , Dovizio Melania , Veronesi Chiara , Degli Esposti Luca , Pérez de Isla Leopoldo 
Published: August 2024

Abstract

Aims
To compare medication adherence, lipid goal attainment, and healthcare costs between patients receiving a single-pill combination (SPC) vs. a free combination treatment (FCT) of rosuvastatin/ezetimibe (ROS/EZE) in Italy.

Methods and results
Administrative databases of healthcare entities covering ∼7 million individuals were used to identify adults prescribed with ROS/EZE as SPC or FCT between January 2018 and June 2020. Adherence was calculated as the proportion of days covered (PDC) after cohort balancing by propensity score matching. Patients with available LDL cholesterol testing were assessed for the proportion of those who at baseline were above lipid targets recommended by ESC/EAS Guidelines for their cardiovascular risk category and reached the target during follow-up. Among 25 886 patients on SPC and 7309 on FCT, adherent patients were more represented in SPC than FCT cohort (56.8 vs. 44.5%, P &lt; 0.001), and this difference remained significant (P &lt; 0.001) after stratification by cardiovascular risk (very high, high, and other). The proportion of patients reaching LDL cholesterol target at 1 year follow-up was significantly (P &lt; 0.001) higher in SPC vs. FCT cohort: 35.4 vs. 23.8% for very high cardiovascular risk, 46.9 vs. 23.1% for high risk and 71.6 vs. 49.5% for other risk. Total healthcare costs per patient at 1 year follow-up were lower in SPC vs. FCT users (2337€ vs. 1890€, P &lt; 0.001). In both cohorts, costs were mainly driven by drug expenses and hospitalizations.

Conclusion
This real-world analysis in dyslipidaemic patients found that treatment with ROS/EZE as SPC resulted in better adherence, higher chances of reaching lipid goals, and cost savings over FCT, in all cardiovascular risk categories.
Accumulation of dually targeted StGPT1 in chloroplasts mediated by StRFP1, an E3 ubiquitin ligase, enhances plant immunity
Authors: Wu Xintong , Zhou Xiaoshuang , Lin Tianyu , Zhang Zhe , Wu Xinya , Zhang Yonglin , Liu Yanli , Tian Zhendong 
Published: August 2024

Abstract
Chloroplasts play a crucial role in essential processes, such as photosynthesis and the synthesis of primary and diverse secondary metabolites. Recent studies have also highlighted their significance linked to phytohormone production in plant immunity, especially SA and JA. Ubiquitination, a key posttranslational modification, usually leads to target protein degradation, which acts as a signal for remodeling the proteome via the induction of protein endocytosis or targeting to other membrane associated systems. Previously, the potato E3 ligase StRFP1 was shown to enhance resistance against Phytophthora infestans, but its mechanism remained unclear. Here, we demonstrate that StRFP1 interacted with the dually localized plastid glucose 6-phosphate transporter StGPT1 on the endoplasmic reticulum (ER). Transiently expressed StGPT1-GFP located on the chloroplast and ER in plant cells. Overexpression of StGPT1 enhances late blight resistance in potato and Nicotiana benthamiana, activates immune responses, including ROS bursts and up-regulation of PTI marker genes. The resistance function of StGPT1 seems to be related to its dual localization. Remarkably, StRFP1 ubiquitinates StGPT1 at the ER, possibly due to its merely transient function in peroxisomes, leading to apparent accumulation in chloroplasts. Our findings point to a novel mechanism by which a plant E3 ligase contributes to immunity via interacting with dually targeted GPT1 at the ER of plant cells.
First joint X-ray solar microflare observations with NuSTAR and Solar Orbiter/STIX
Authors: Bajnoková Natália , Hannah Iain G , Cooper Kristopher , Krucker Säm , Grefenstette Brian W , Smith David M , Jeffrey Natasha L S , Duncan Jessie 
Published: August 2024

ABSTRACT
We present the first joint spectral and imaging analysis of hard X-ray (HXR) emission from three microflares observed by the Nuclear Spectroscopic Telescope ARray (NuSTAR) and Solar Orbiter/Spectrometer/Telescope for Imaging X-rays (STIX). We studied 5 joint spectra from GOES A7, B1, and B6 class microflares from active region AR12765 on 2020 June 6 and 7. As these events are very bright for NuSTAR, resulting in extremely low (&lt;1 per cent) livetime, we introduce a pile-up correction method. All five joint spectra were fitted with an isothermal model finding temperatures in the 9–11 MK range. Furthermore, three joint spectra required an additional non-thermal thick-target model finding non-thermal powers of $10^{25}$–$10^{26}$ erg s$^{-1}$. All the fit parameters were within the ranges expected for HXR microflares. The fit results give a relative scaling of STIX and NuSTAR mostly between 6 and 28 per cent (one outlier at 52 per cent) suggesting each instrument are well calibrated. In addition to spectral analysis, we performed joint HXR imaging of the June 6 and one of the June 7 microflares. In NuSTAR’s field of view (FOV), we observed two separate non-thermal sources connected by an elongated thermal source during the June 6 microflares. In STIX’s FOV (44$^{\circ }$ W with respect to NuSTAR), we imaged thermal emission from the hot flare loops which when reprojected to an Earth viewpoint matches the thermal sources seen with NuSTAR and in the hotter EUV channels with the Solar Dynamic Observatory’s Atmospheric Imaging Assembly.
Clinical features of juvenile onset ankylosing spondylitis in Japanese patients
Authors: Kishimoto Kenji , Asai Shuji , Suzuki Mochihito , Sato Ryo , Hasegawa Junya , Terabe Kenya , Imagama Shiro 
Published: August 2024

ABSTRACT

Objectives
This retrospective study aimed to examine the clinical features of juvenile onset ankylosing spondylitis (JoAS) in Japanese patients.

Methods
We examined clinical symptoms (including initial symptoms) and the progression to diagnosis of AS in 17 Japanese JoAS patients at Nagoya university hospital between January 2004 and May 2023. Initial symptoms considered were pain at axial joints and/or extra-axial joints.

Results
Mean ages (± standard deviation) at onset and diagnosis of AS were 12.9 (± 2.0) and 19.6 (± 9.6) years, respectively. The back was the most common site of initial symptoms (7 patients; 41.2%), followed by the hip (5 patients; 29.4%) and knees (5 patients; 29.4%). Initial symptoms were limited to extra-axial joints and axial joints in 9 (52.9%) and 7 (41.2%) patients, respectively. Nine patients (52.9%) were recognised as a musculoskeletal disease other than AS, such as oligoarticular juvenile idiopathic arthritis.

Conclusions
Sites of initial symptoms frequently were the back, hip, and knees, with 52.9% of patients having initial symptoms limited to extra-axial joints. More than half of the patients recognised musculoskeletal diseases other than AS.
Expanding super-resolution imaging versatility in organisms with multi-confocal image scanning microscopy
Authors: Ren Wei , Guan Meiling , Liang Qianxi , Li Meiqi , Jin Boya , Duan Guangxing , Zhang Liya , Ge Xichuan , Xu Hong , Hou Yiwei , Gao Baoxiang , Sodmergen  , Xi Peng 
Published: August 2024

ABSTRACT
Resolving complex three-dimensional (3D) subcellular dynamics noninvasively in live tissues demands imaging tools that balance spatiotemporal resolution, field-of-view and phototoxicity. Image scanning microscopy (ISM), as an advancement of confocal laser scanning microscopy, provides a 2-fold 3D resolution enhancement. Nevertheless, the relatively low imaging speed has been the major obstacle for ISM to be further employed in in vivo imaging of biological tissues. Our proposed solution, multi-confocal image scanning microscopy (MC-ISM), aims to overcome the limitations of existing techniques in terms of spatiotemporal resolution balancing by optimizing pinhole diameter and pitch, eliminating out-of-focus signals, and introducing a frame reduction reconstruction algorithm. The imaging speed is increased by 16 times compared with multifocal structured illumination microscopy. We further propose a single-galvo scan, akin to the Archimedes spiral in spinning disk confocal systems, to ensure a high-speed and high-accuracy scan without the galvanometer's inertial motion. Benefitting from its high photon efficiency, MC-ISM allows continuous imaging of mitochondria dynamics in live cells for 1000 frames without apparent phototoxicity, reaching an imaging depth of 175 μm. Noteworthy, MC-ISM enables the observation of the inner membrane structure of living mitochondria in Arabidopsis hypocotyl for the first time, demonstrating its outstanding performance.
Direct propylene epoxidation with molecular oxygen over titanosilicate zeolites
Authors: Li Weijie , Qin Bin , Dong Zhuoya , Chai Yuchao , Wu Guangjun , Ma Yanhang , Wang Meng , Liu Xingwu , Ma Ding , Li Landong 
Published: August 2024

ABSTRACT
The direct epoxidation of propylene with molecular oxygen represents a desired route for propylene oxide (PO) production with 100% theoretical atomic economy. However, this aerobic epoxidation reaction suffers from the apparent trade-off between propylene conversion and PO selectivity, and remains a key challenge in catalysis. We report that Ti-Beta zeolites containing isolated framework Ti species can efficiently catalyze the aerobic epoxidation of propylene. Stable propylene conversion of 25% and PO selectivity of up to 90% are achieved at the same time, matching the levels of industrial ethylene aerobic epoxidation processes. H-terminated pentacoordinated Ti species in Beta zeolite frameworks are identified as the preferred active sites for propylene aerobic epoxidation and the reaction is initiated by the participation of lattice oxygen in Ti-OH. These results are expected to spark new technology for the industrial production of PO toward more sustainable chemistry and chemical engineering.
Association of COVID-19 Vaccination With Risk of Medically Attended Postacute Sequelae of COVID-19 During the Ancestral, Alpha, Delta, and Omicron Variant Eras
Authors: Swift Melanie D , Breeher Laura E , Dierkhising Ross , Hickman Joel , Johnson Matthew G , Roellinger Daniel L , Virk Abinash 
Published: August 2024

Abstract

Background
Uncertainty exists regarding the effectiveness of COVID-19 vaccine to prevent postacute sequelae of COVID-19 (PASC) following a breakthrough infection. While most studies based on symptom surveys found an association between preinfection vaccination status and PASC symptoms, studies of medically attended PASC are less common and have reported conflicting findings.

Methods
In this retrospective cohort of patients with an initial SARS-CoV-2 infection who were continually empaneled for primary care in a large US health system, the electronic health record was queried for preinfection vaccination status, demographics, comorbidity index, and diagnosed conditions. Multivariable logistic regression was used to model the outcome of a medically attended PASC diagnosis within 6 months of SARS-CoV-2 infection. Likelihood ratio tests were used to assess the interaction between vaccination status and prevalent variant at the time of infection and between vaccination status and hospitalization for SARS-CoV-2 infection.

Results
During the observation period, 6.9% of patients experienced medically attended and diagnosed PASC. A diagnosis of PASC was associated with older age, female sex, hospitalization for the initial infection, and an increased severity-weighted comorbidity index and was inversely associated with infection during the Omicron period. No difference in the development of diagnosed PASC was observed between unvaccinated patients and those vaccinated with either 2 doses of an mRNA vaccine or &gt;2 doses.

Conclusions
We found no association between vaccination status at the time of infection and development of medically diagnosed PASC. Vaccine remains an important measure to prevent SARS-CoV-2 infection and severity. Further research is needed to identify effective measures to prevent and treat PASC.
Assessing the adoption and utilization of the Rwanda COVID-19 data analytics system: a mixed methods approach
Authors: Mugabo Hassan , Rukundo Gilbert , Ngabonziza Jean Claude S , Mazarati Jean-Baptiste , Aghatise Joseph , Akinwusi Olukunle 
Published: August 2024

Abstract
Introduction: Rwanda has been widely lauded for its exceptional response to the COVID-19 pandemic. However, although Rwanda established a national system for COVID-19 testing and vaccination data, concerns have been raised about data fragmentation which requires linkage of various data sources, access to data for real-time decision-making, and data completeness. Methods: We assessed the adoption of the Rwanda COVID-19 data Analytics System (RCAS) for public health staff that employ data from various platforms to generate evidence for policy- and decision-making. A random sample of 56 participants was drawn from the 98 who attended the 2022 RCAS training for data managers from the Rwanda Biomedical Center, technical partners, and health facilities. Of the selected participants, 42 completed the online self-administered questionnaire within the 14-day data collection period. Key informant interviews were then conducted with a subset of 14 respondents. Results: A strong positive relationship (χ2 = 9.1049, P &lt; 0.05) emerged between respondents' decision-making regarding RCAS and their support for its sustainability. There was a marginal association (χ2 = 3.3358, P = 0.059) suggesting a link between users' ease of data exchange through RCAS and their support for its long-term sustainability, warranting further exploration. Conclusion: RCAS had a positive impact on improvements in data linkage, access to individual-level data for analyses, and progress toward harmonization of health data beyond COVID-19 in Rwanda. Users noted the usability, acceptability, and interoperability of the system. Recommendations for further improvement and scaling of the intervention are discussed.
It Takes a Movement: Lawyers, Litigation, and Legal Theory in the Attack on Campaign Finance Reform
Authors: Briffault Richard 
Published: August 2024

Abstract
Recent Supreme Court decisions have hobbled campaign finance regulation and placed significant constitutional constraints on possible future reforms. But the Supreme Court has not acted alone. Major constitutional changes often require a substantial legal and political infrastructure, an ecosystem of actors, ideas, strategies, and tactics to instantiate new constitutional doctrine into law. In Big Money Unleashed, Ann Southworth examines the composition, development, organization, and intellectual underpinning of the legal movement that has worked to cripple campaign finance regulation. She chronicles the raising and testing of the key legal theories; the sources of political and financial backing for the movement; the combination of disparate groups into an antireform coalition; and the organization of what has proven to be a very effective litigation strategy. The result, she explains, has been the ascendancy of a powerful constitutional discourse that treats money as speech, campaign spending as freedom, and government regulation as censorship. The antireform litigation campaign on its own would not have been enough to transform the law; key appointments to the Supreme Court were essential. But the movement provided the Court with the cases, the arguments, and broader political support that are part of constitutional change.
Using Neuromodulators for Salivary, Eccrine, and Apocrine Gland Disorders
Authors: Rajanala Susruthi , Salame Nicole , Dover Jeffrey S. 
Published: August 2024

BACKGROUND
Sialorrhea, hyperhidrosis, bromhidrosis, and chromhidrosis are common glandular disorders that substantially impact patients' health and quality of life. Botulinum toxin can safely and temporarily decrease gland secretions by targeting the parasympathetic cholinergic neurons, resulting in diminished saliva and sweat production.

OBJECTIVE
The objective of this article is to describe the applications of neuromodulators for the treatment of salivary, eccrine, and apocrine glands.

METHODS
PubMed was searched from inception to February 1, 2024 using search terms “neurotoxin,” “botulinum toxin,” “sialorrhea,” “hyperhidrosis,” “bromhidrosis,” and “chromhidrosis.”

RESULTS
Incobotulinumtoxin A and Rimabotulinumtoxin B are approved by the FDA for the treatment of sialorrhea. Onabotulinumtoxin A is the only FDA-approved botulinum toxin for axillary hyperhidrosis and is used off-label for hyperhidrosis of nonaxillary sites, bromhidrosis, and chromhidrosis. Compared to botulinum toxin serotype A, serotype B has been associated with more immunogenicity, which may have implications for patients requiring long-term treatment for chronic glandular disorders.

CONCLUSION
Neuromodulators are safe and effective for the noninvasive treatment of excess gland activity and can improve patients' quality of life. While substantial literature supports botulinum toxin treatments for hyperhidrosis, further studies are needed to characterize standard dosing and administration techniques for sialorrhea, bromhidrosis, and chromhidrosis.
Serum protein responses to Dietary Approaches to Stop Hypertension (DASH) and DASH-Sodium trials and associations with blood pressure changes
Authors: Kim Hyunju , Lichtenstein Alice H. , Coresh Josef , Appel Lawrence J. , Rebholz Casey M. 
Published: August 2024

Objectives:
The Dietary Approaches to Stop Hypertension (DASH) diet reduces blood pressure, but the mechanisms underlying DASH diet-blood pressure relations are not well understood. Proteomic measures may provide insights into the pathophysiological mechanisms through which the DASH diet reduces blood pressure.

Methods:
The DASH (1994–1996) and DASH-Sodium (1997–1999) trials were multicenter, randomized-controlled feeding trials. Proteomic profiling was conducted in serum collected at the end of the feeding period (DASH, N = 215; DASH-Sodium, N = 390). Multivariable linear regression models were used to identify interactions between 71 DASH diet-related proteins and changes in systolic and diastolic blood pressure. Estimates were meta-analyzed across both trials. Elastic net models were used to identify proteins that predict changes in blood pressure.

Results:
Ten significant interactions were identified [systolic blood pressure: seven proteins; diastolic blood pressure: three proteins], which represented nine unique proteins. A high level of renin at the end of the feeding period was associated with greater reductions in diastolic blood pressure in individuals consuming the control than DASH diets. A high level of procollagen c-endopeptidase enhancer 1 (PCOLCE) and collagen triple helix repeat-containing protein 1 (CTHRC1) were associated with greater reductions in systolic blood pressure in individuals consuming the DASH than control diets, and with elevations in systolic blood pressure in individuals consuming the control diets (P for interaction for all tests < 0.05). Elastic net models identified six additional proteins that predicted change in blood pressure.

Conclusions:
Several novel proteins were identified that may provide some insight into the relationship between the DASH diet and blood pressure.
Trajectory of Telework in a Brazilian Labor Court During the COVID-19 Pandemic
Authors: El Kadri Filho Fauzi , de Lucca Sérgio Roberto 
Published: August 2024

Objective
This study aimed to compare ergonomic and psychosocial risks and the prevalence of musculoskeletal problems and common mental disorders during the coronavirus disease 2019 pandemic according to work modality.

Methods
A longitudinal study was carried out with 659 employees between 2021 and 2022. The data were collected via web with self-administered questionnaires. The Wilcoxon and repeated-measure analysis of variance tests were used.

Results
The participants who started teleworking during the pandemic showed significant improvements in their workstations and worsening of the risks associated with rest breaks after returning to face-to-face work. Employees in telework presented a significant reduction in the intensity of musculoskeletal complaints in the last 7 days compared to those who returned to face-to-face work.

Conclusions
Although there was a reduction in the ergonomic risk after returning to face-to-face work, telework was related to better working and health conditions.
Modern Oncologic Maxillary Reconstruction
Authors: Morrison Edwin J. , Matros Evan 
Published: August 2024

Learning Objectives:
After studying this article, the participant should be able to: (1) Have a broad understanding of the oncological principles relating to cancers involving the maxilla. (2) Define anatomically the various types of maxillectomy defects and their associated morbidity. (3) Understand the goals and principles of maxillary reconstruction. (4) Demonstrate an understanding of the strengths, limitations, and alternative reconstructive options for the various types of maxillectomy defects.

Summary:
Reconstruction of the maxilla following cancer resection has evolved over the past decade. Underpinned by advances in virtual surgery technology and an increased focus on dental rehabilitation, more sophisticated workflows using free bone flaps have become more commonplace. There are few reconstructions anywhere in the body that require the restoration of similarly intricate bone and soft-tissue relations with implications for both form and function, like the maxilla. Success demands careful anatomical definition of the defect, an understanding of the goals and principles of reconstruction, and the understanding of relative strengths and limitations of the various reconstructive options. Equally, as no one technique is without shortcomings and maxillary surgery can be associated with a significant complication profile, it is just as important to match the patient and oncologic context to the reconstruction. This article is intended to provide the reader with a broad overview of these core factors.