bluuebunny/crossref_metadata_2025_split huggingface dataset:
Focal Gaps in the Basement Membrane of Involved and Uninvolved Skin of Vitiligo: Are They Normal?
Authors: Bose Sumit Kumar , Ortonne Jean‐Paul 
Published: April 2015

AbstractIt has been suggested that, in vitiligo, the structural abnormalities may not be restricted to melanocytes alone, but also to keratinocytes of involved and uninvolved skin. Focal gaps in the vitiligo epidermal basement membrane have been furthermore interpreted as evidence of extramelanocyte alterations. To understand the occurrence of the gaps we undertook a study to establish which cells are related to them. Immunofluorescence and immunoperoxidase techniques were used to visualize, in involved, uninvolved, and control skin, the basement membrane, melanocytes, and Merkel cells. Double staining was performed with fluorescein‐coupled anti‐collagen IV antibodies and rhodamin‐coupled antibodies against Nicein (GB3), a hemidesmosome component Propidium iodide staining was used to detect infiltrating lymphocytes.The basement membrane structure was always found to be intact and unaltered. Absence of staining in areas of uninvolved skin was related to the presence of Merkel cells, melanocytes, and infiltrating lymphocytes. Gaps in the epidermal basement membrane are thus due to lack of staining caused by the presence of immigrant cells.
A Case of Mammary Paget's Disease without an Underlying Carcinoma: Microscopic Analysis of the DNA Content in Paget Cells
Authors: Mori Osamu , Hachisuka Hiroshi , Nakano Shunji , Maeyama Yasuhiko , Sasai Yoichiro 
Published: April 2015

AbstractA 72‐year‐old woman had a 7‐year history of a scaly red area on the right breast which had enlarged asymptomatically. A biopsy of the nipple was taken, and, following the finding of Paget's disease, a modified radical mastectomy was carried out. On histological examination of the entire breast specimen by serial sections, no evidence of an intraductal adenocarcinoma was found.Fractionation of Paget cells was performed from the epidermis. Stripped skin was treated with EDTA and trypsin, and epidermal cell suspensions were obtained. They were layered onto discontinuous Percoll gradients and centrifuged. Paget cells fell into three fractions with densities of 1.041, 1.058, and 1.078. Electronmicroscopically, the purity of fractionated cells obtained by this method ranged from 55 to 74% with viabilities of from 70 to 90%. Microscopic analysis of the DNA content in these cells was performed. The DNA histogram was close to the normal ploidy. This may explain why the mammary Paget's disease lesions in this case enlarged rather slowly.
The Antibiotic Susceptibility of Propionibacterium acnes and Staphylococcus epidermidis Isolated from Acne
Authors: Nishijima Setsuko , Akamatsu Hirohiko , Akamatsu Maki , Kurokawa Ichiro , Asada Yasuo 
Published: April 2015

AbstractWe studied the susceptibility of antimicrobial agents to Propionibacterium acnes (P. acnes) and Staphylococcus epidermidis (S. epidermidis) isolated from acne patients.We measured the minimum inhibitory concentrations (MICs) of the following five drugs: roxithromycin (RXM), erythromycin (EM), clindamycin (CLDM), minocycline (MINO) and ofloxacin (OFLX), which are frequently Used to treat acne and skin infections. We found many resistant strains of S. epidermidis and some resistant strains of P. acnes. There was a correlation between the resistance of S. epidermidis and the former therapy for acne, but no distinct correlation between the resistance of P. acnes and the former therapy for acne.
Induction of Intercellular Adhesion Molecule‐1 and Adherence of HTLV‐1‐Infected T‐cells to Cultured Keratinocytes
Authors: Fenxiang Jin , Iwatsuki Keiji , Takigawa Masahiro , Yamada Mizuho 
Published: April 2015

AbstractCutaneous lesions of T‐cell proliferative disorders are characterized by epidermotropic infiltration of the neoplastic cells and expression of intercellular adhesion molecule‐1 (ICAM‐1) and HLA‐DR by lesional keratinocytes. Using cloned HTLV‐1‐infected T‐cells obtained from patients with adult T‐cell leukemia (ATL), we have studied immunobiological activities of cytokines released from the T‐cell lines and their ability to adhere to cultured keratinocytes. Three out of the five CD‐4‐positive, HTLV‐1‐infected T‐cell clones secreted both IFN‐γ and IL‐4, similar to murine Th0 clones. The other two clones did not produce such cytokines. ICAM‐1 and HLA‐DR molecules were induced on cultured normal human keratinocytes and organ‐cultured skin specimens by co‐cultivation with IFN‐γ‐producing T‐cell clones or their culture supernatants. Induction of both molecules was markedly inhibited by pretreatment of the supernatants with excess amounts of anti‐IFN‐γ monoclonal antibody. The number of cells adherent to the normal cultured keratinocytes was greater in the IFN‐γ‐producing clones than in the non‐producing ones. These data suggest that some HTLV‐1‐infected clones produce cytokines, including IFN‐γ, which in turn induce ICAM‐1 on keratinocytes, thereby enhancing the ability of the T‐cell clones to adhere to the keratinocytes.
Cutaneous Arterial Fibromuscular Dysplasia: A Case Report and Electron‐microscopic Study
Authors: Setoyama Mitsuru , Shimada Tokihiko , Kanzaki Tamotsu , Moriya Kazuhiro , Okahara Kazunori , Asakura Tetsuhiko 
Published: April 2015

AbstractA case of a 39‐year‐old female with fibromuscular dysplasia (FMD) manifesting subcutaneous pulsatile nodules on the right side of her forehead and on her right wrist is reported here. These nodules proved to be FMD aneurysms of the frontal ramus of the right superficial temporal artery and the right radial artery. The patient had also suffered a stroke with subarachnoidal bleeding as a result of this disease.Angiographic examination showed aneurysms in the union and the bilateral vertebral arteries, a branch of the right renal artery, and one the right lumbar artery. A biopsy specimen taken from the frontal branch of the right superficial artery revealed segmental intimal thickening consistent with intimal fibroplasia type FMD upon histological examination. Electron‐microscopic findings were also discussed in connection with the histogenesis of the disease.This appears to be the second case of FMD involving arteries in the skin to be reported in the dermatological literature.
Pemphigus Developed on Preexisting Dermatoses
Authors: Lee Chang Woo , Ro Young Suck 
Published: April 2015

AbstractA case of pemphigus foliaceus which initially developed at sites of preexisting psoriasis and a case of pemphigus erythematosus that developed on the lesions of photosensitive erythemas are described.Inflammatory dermatoses can be considered to be local factors which facilitate the autoimmune response of pemphigus, which in turn may induce blistering cutaneous lesions in subclinical patients with autoantibodies.
Sensitivity of Staphylococcus aureus and Streptococcus pyogenes Isolated from Skin Infections in 1992 to Antimicrobial Agents
Authors: Nishijima Setsuko , Namura Shoko , Kawai Shuzou , Akamatsu Hirohiko , Asada Yasuo , Kawabata Sigekatsu 
Published: April 2015

AbstractWe studied the efficacy of antimicrobial agents against Staphylococcus aureus (S. aureus) and Streptococcus pyogenes (S. pyogenes) isolated from skin infections in 1992.For S. aureus, we measured the minimum inhibitory concentrations (MICs) of the following 10 drugs: methicillin (DMPPC), cefaclor (CCL), gentamicin (GM), erythromycin (EM), clindamycin (CLDM), minocycline (MINO), vancomycin (VAN), fusidic acid (FA), ofloxacin (OFLX) and nadifloxacin (NDFX); for S. pyogenes, we determined the MICs of the following 9 drugs: ampicillin (ABPC), amoxicillin (AMPC), cefpodoxime proxetil (CPDX‐PR), erythromycin (EM), clindamycin (CLDM), minocycline (MINO), norfloxacin (NFLX), ofloxacin (OFLX) and nadifloxacin (NDFX). These drugs are frequently used to treat skin infections, either systemically or topically. NDFX is a new synthetic fluoroquinolone, recently developed for use as a topical acne medication in Japan. It is used NDFX for acne, but not for skin infections.There were no strains of S. aureus resistant to NDFX, VAN or FA. The resistance (≧12.5 µg/ml) of S. aureus was highest to GM and lowest to OFLX. Four strains of methicillin‐resistant (≧12.5 µg/ml) S. aureus (MRSA) were found.In contrast, no resistant strains of S. pyogenes were found except to MINO. Only two strains of S. pyogenes were susceptible to MINO. The sensitivity of S. pyogenes to ABPC, AMPC, CPDX‐PR, EM and CLDM was very good. All the strains were susceptible at a MIC below ≧0.05 µg/ml. However, the S. pyogenes strains were not very sensitive to the new quinolones, especially NFLX.We concluded that penicillins, cephalosporins and macrolides are still effective against streptococcal infections. In contrast, we found S. aureus to be resistant to several kinds of antimicrobial agents. Accordingly, we need to check the sensitivity of S. aureus, before using antimicrobial agents for staphylococcal infections.Roughly speaking, the drugs that are strongly effective against S. aureus are not effective against S. pyogenes. For example, the efficacy of MINO against S. aureus was very good, but almost all S. pyogenes strains were resistant to it. The pattern is much the same for the new quinolones.
Natural Cellular Cytotoxicity in Behçet's Disease
Authors: Önder Meltem , Bozkurt Meral , Gürer Mehmet Ali , Gülekon Ayla , Sezgin Pervin , İmir Turgut 
Published: April 2015

AbstractThis study evaluated the cytotoxic activity of natural killer cells in the active and inactive stages of Behçet's disease (BD) and attempted to develop a new explanation for its immuno‐pathogenesis. Blood samples were taken from 16 BD patients and compared with 11 healthy individuals. The lymphocyte fraction was separated and diluated in RPMI‐1640. Candida as a target cell (T) was mixed with lymphocytes (E) (effector cells) in ratios of T:E 1/5 and T:E 1/25. After the numbers of colonies were counted with controls, the anticandidal index (natural cytotoxicity) was calculated. Natural cytotoxicity relatively decreased in the active stage and increased in the inactive stage of BD. Although the difference between the mean value of natural cytotoxicity in the active stage and in the inactive stage was significant, the difference between the averages of active stage and the control group was insignificant. However, the difference between inactive stage and the control group was remarkable. The increase of the natural cytotoxic activity in the inactive period of the disease may play a role together with other immune mechanisms in the aetiopathogenesis of BD.
A Case of Chronic GVHD Following Bone Marrow Transplantation from a Phenotypically HLA‐Matched Unrelated Donor
Authors: Sano Yoshimi , Tanaka Keiko , Shimao Shuhei 
Published: April 2015

AbstractA 45‐year‐old male with chronic myelocytic leukemia who received a bone marrow transplantation from a phenotypically HLA‐matched unrelated donor developed chronic GVHD on day 100 post transplantation. He developed a slight fever, malaise, hepatic dysfunction and extensive itchy erythema with scaling over his entire body. The inflammatory skin lesion developed into erythroderma in about two weeks. H&E staining of a skin biopsy revealed eosinophilic bodies and a lymphocytic infiltration in the dermis and epidermis, which were compatible with the early phases of chronic GVHD. Immunohistochemistry revealed that keratinocytes expressed dense HLA‐DR and ICAM‐1 epitopes. Langerhans cells (CD1a+ cells) had disappeared from the epidermis. Many T cells (CD3+ cells) had migrated into the epidermis as well as into the reticular dermis. The majority of the T cells in the epidermis were CD8+ cells, while almost all the T cells in the dermis were CD4+ cells. These immunohistochemical features were similar to those previously reported for acute cutaneous GVHD. Despite the corticosteroid therapy, the eruptions did not disappear. The patient was then treated with whole body bath‐methoxsalen (Oxsoralen®) plus ultraviolet A (UVA). The bath‐psoralen plus UVA therapy was effective in this patient.
A Case of Superficial Epithelioma with Sebaceous Differentiation
Authors: Akasaka Toshihide , Imamura Yuko , Tomichi Nobukazu , Kon Saiichi 
Published: April 2015

AbstractSuperficial epithelioma with sebaceous differentiation developed on the left cheek of a 58‐year‐old man over a three‐year period. Biopsy of the lesion demonstrated plate‐like lobules of basophilic basaloid cells with broad attachments to the overlying epidermis. Clusters of or solitary sebaceous cells were present within the lobules. Three tumor types were considered; a subtype of sebaceoma growing in the epidermis, an acanthotic seborrheic keratosis subtype with sebaceous differentiation, or a tumor of the follicular infundibulum with sebaceous differentiation.
A Case of Generalized Lichen Nitidus with Koebner's Phenomenon
Authors: Maeda Manabu 
Published: April 2015

AbstractA 12‐year‐old boy was seen in our department with a three year history of eruptions on his back consisting of normal colored or slightly reddish, pinhead‐sized papules, which had gradually spread to his trunk within the previous year. Four days before the first visit, May 13, 1992, the eruption involved his entire extremities. On the back side of his lumbar region, Koebner's phenomenon was observed. He had received several medications for more than two years to treat this eruption in several hospitals, but none were effective. He was in good general health. Mantoux reaction was negative two months after BCG injection. Histopathologic features of the papules included focal epidermal hyperplasia and elongation of the rete ridges in a narrow area and a well demarcated dense lymphohistiocytic infiltrate on the papillar layer. Biscoclaurine alkaloids (20 mg/day) and Jumi‐haidoku‐to (TJ‐6; 7.5 g/day) were administered to the patient after the biopsy. No topical ointments were applied. Two weeks after of these treatments, he reported moderate pruritus on the back. The eruption diminished rapidly within 2 weeks after the therapy began. Almost all the eruptions were cured within one year. Mantoux reaction developed 8 times 8 mm erythema two and half months after the treatment began, and it was significantly positive (23 times 30 mm) 6 months later.
Accessory Tragi: A Case Report
Authors: Sehgal Virendra N. , Chander Ram , Garg Vijay K. , Jain Sanjiv , Logani Kulbhushan 
Published: April 2015

AbstractAccessory tragi, a fascinating supernumerary embryonic accessory in a 3‐year‐old child, is described, emphasizing the cardinal clinical features and histopathological characteristics.
A Case of Fibrous Histiocytoma Associated with Xanthogranulomatous Alteration
Authors: Maruyama Michiyo , Sato Shinichi , Tsuchida Tetsuya , Toda Kiyoshi 
Published: April 2015

AbstractWe report here an unusual case of fibrous histiocytoma in which an area of xanthogranuloma was observed histologically. Our case suggests that adult xanthogranuloma is a variant of fibrous histiocytoma.
In situ Expression of Interleukin‐6 in Psoriatic Epidermis during Treatment
Authors: Ohta Yukinori , Nishiyama Shigeo , Nishioka Kiyoshi 
Published: April 2015

AbstractInterleukin‐6 (IL‐6) is a multipotential cytokine which may act as a growth factor for keratinocytes. The epidermal hyperplasia of psoriasis may be explained in part by an overproduction of this cytokine. We have previously shown by in situ hybridization that IL‐6 mRNA is most strongly expressed in the peripheral lesion of an advancing psoriatic plaque. In the present study, we investigated whether there were differences between the expression of IL‐6 in untreated psoriatic epidermis and the lesion during the course of clinical improvement. In the untreated psoriatic lesion, the weak expression of IL‐6 mRNA was localized in the lower epidermis. However, IL‐6 mRNA was not detected in the clearly improved lesion. In the improving lesion, with clinically less scaling, less induration, and histologically thinner epidermis, IL‐6 mRNA‐expressing keratinocytes were detectable in a greater proportion of the total epidermal components than in the untreated, fully developed lesion. These results showed that IL‐6 mRNA was strongly expressed in lesions with moderate epidermal hyperproliferation, indicating that this cytokine may play a role in the transitional phase during the course of improvement as well as in the lesion's formation.
Bone Mineral Density of the Lumbar Spine in Psoriatic Patients with Long Term Etretinate Therapy
Authors: Okada Natsuko , Nomura Masao , Morimoto Shigeto , Ogihara Toshio , Yoshikawa Kunihiko 
Published: April 2015

AbstractEtretinate has been known to produce a variety of skeletal manifestations when administered for prolonged periods. To study whether osteoporotic changes occurred during the treatment, bone mineral density (BMD) was measured by dual energy X‐ray absorptiometry in 13 psoriatic patients who received long term etretinate therapy (average 3.7 yrs). The values of BMD of the lumbar spine in these patients were decreased from those of age‐matched controls (92.3 ± 9.7%, p<0.01), while those in psoriatic patients who received topical corticosteroid applications alone showed no significant decrease. Our findings suggest that long term etretinate intake induces an increased risk of osteoporotic changes.
Hair Fibrous Protein Compositions in Some Hereditary Hair Abnormalities Determined by Two‐Dimensional Polyacrylamide Gel Electrophoresis
Authors: Koike Shun'ichi , Dekio Satoshi , Jidoi Joji , Yoshimura Yasuro , Ishii Masamitsu , Hamada Toshio , Saito Ryuzo 
Published: April 2015

AbstractS‐carboxymethylated (SCM) fibrous proteins (FPs) from the scalp hairs of the three different hereditary hair abnormalities (trichorrhexis invaginata in Netherton's syndrome, pili trianguli et canaliculi in uncombable hair syndrome, and fine hair in anhidrotic ectodermal dysplasia) were analyzed by two‐dimensional polyacrylamide gel electrophoresis. Comparison of the SCM FP compositions of the three hair abnormalities with that of the normal revealed that the SCM FP compositions of these three abnormal hairs were all electrophoretically different from each other and that of the normal.
Congenital Rubella Syndrome with Rubella Virus‐associated Generalized Brownish Macules, Indurated Erythemas, Papules, and Pigmentation
Authors: Seno Akemi , Tada Joji , Matsuura Hironori , Arata Jirô , Isomura Hiroki , Kitamura Tetsuro , Oda Megumi , Katow Shigetaka 
Published: April 2015

AbstractBackgroundWe examined an infant with congenital rubella syndrome (CRS). The purpose of this report is to describe the skin manifestations in this patient and to prove that they were associated with rubella virus.ObservationsA 7‐month‐old boy presented with generalized brownish macules, indurated erythemas, papules, and pigmentation. They first appeared at around 3 months of age. His mother had contracted rubella during the 14th gestational week. At the time of examination, rubella‐specific IgM antibody was positive in both serum and cerebrospinal fluid of the baby. A physical exam had revealed deafness, mental and physical retardation, interstitial pneumonitis, and hepatosplenomegaly. A skin biopsy specimen showed a dense infiltration mainly of lymphocytes, with B cells predominant in the deep dermis. Electron microscopically abundant tubuloreticular structures were observed in capillary endothelial cells, lymphocytes, and dermal fibroblasts. Polymerase chain reaction (PCR) analysis suggested that rubella virus RNA was present in the patient's skin specimen, cerebrospinal fluid, and total blood.ConclusionsThe cutaneous manifestations of our patient were extraordinary and informative. These prominent skin lesions should be recognized as cutaneous markers of CRS.
Photocontact Allergy Due to Suprofen
Authors: Kuno Yoshinori , Numata Tokio 
Published: April 2015

AbstractA 79‐year‐old Japanese woman developed edematous erythema on sun‐exposed areas 3 months after applying Sulprotin® ointment, which contains 1% suprofen (SP), a phenylacetic acid derivative. A patch test with Sulprotin® ointment as is was negative, but a photopatch test with Sulprotin® ointment as is was positive. A photopatch test with 10–3% SP was positive, as was that obtained with an SP analogue, 3% ketoprofen, but that with the ointment base of Sulprotin® was negative. Photopatch tests with other SP analogues such as flurbiprofen, ibuprofen, and pranoprofen were negative. She developed abnormal erythema with monochromatic irradiation in the range from 320 to 380 nm on the uninvolved abdominal skin where 1% SP had been applied 24 hours (h) before irradiation. The action spectrum for this erythema agreed with the absorption spectrum of SP in the range of UVA. The MED after applying SP was below that after applying w. pet. in the range from 250 to 290 nm and from 300 to 320 nm. Positive intracutaneous test results were obtained with an irradiated mixture of human serum albumin (HSA) and SP at 48 and 72 h after injection and negative ones with a mixture of HSA and irradiated SP.
Melanosis Riehl‐like Facial Pigmentation in a Japanese Case of AIDS
Authors: Hanada Katsumi , Hashimoto Isao , Baba Takako , Tamura Toyokazu , Kishibe Susumu 
Published: April 2015

AbstractAn AIDS patient with the cardinal complaint of severe pigmentation on the face is reported. Hyperpigmentation is an unusual symptom of AIDS. This is a Japanese patient who presented melanosis Riehl‐like discoloration. The importance of pigmented lesions in patients who have not been given any anti‐HIV agents has not been emphasized in the literature.
Relationship between Porphyria Cutanea Tarda (PCT) and Viral Hepatitis
Authors: Tsukazaki Naoko , Tanaka Keiichi , Irifune Hiroko , Yoshida Hikotaro , Watanabe Masahisa , Ohgami Taro , Nonaka Shigeo 
Published: April 2015

AbstractRecent reports have revealed the high prevalence of serological markers of viral hepatitis in porphyria cutanea tarda (PCT). We present two cases of PCT associated with hepatitis C and discuss the relationship between PCT and viral hepatitis. Case 1: A 50‐year‐old Japanese male noticed blisters, erosions, and fragility on sun‐exposed areas of his skin in November of 1990. He had no history of excessive alcohol intake. He had been taking analgesics for eighteen years. Case 2: A 64‐year‐old Japanese male was referred in October of 1989 because of pigmentation on sun‐exposed areas of his skin. He had been drinking alcohol excessively for 43 years. The hepatitis C virus (HCV) antibody was present in each case. Tests for the HCV antibody and hepatitis B serological markers were run in 5 other patients. HCV antibody was present in 3 of them. The two cases negative for the HCV antibody exhibited the hepatitis B antibody. We speculated that viral hepatitis infection may play an important role in precipitating PCT in cases with a history of a long term excessive intake of alcohol or chemicals.
Photoleukomelanodermatitis (Kobori) Induced by Afloqualone
Authors: Ishikawa Takeshi , Kamide Ryoichi , Niimura Michihito 
Published: April 2015

AbstractWe reported a case of photoleukomelanodermatitis (Kobori) type drug eruption due to afloqualone (Arofuto®). The patient was given afloqualone and imipramine hydrochloride (Chrytemin®) for cervical spondylosis from November of 1990. Edematous erythema with slight itching appeared on the sun‐exposed areas in December of 1990. As drug eruption was suspected, drugs were ceased, and the cutaneous lesions almost disappeared but pigmentations and depigmentations developed in spots in sun‐exposed areas in March of 1991. Photopatch and oral challenge tests were positive.