bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lignans and Kauranes from the Stems of Annona cherimola
Authors: Chen Chung‐Yi , Wu Tung‐Ying , Chang Fang‐Rong , Wu Yang‐Chang 
Published: May 2015

AbstractFour lignans and six kauranes, (+)‐epi‐syringaresinol (1), annocherin A (16β‐hydroxy‐17,19‐diacetoxy‐ent‐kaurane) (2), (+)‐syringaresinol (3), (+)‐dia‐syringaresinol (4), liriodendrin[(+)‐syringaresinol‐di‐O‐β‐D‐glucopyranoside] (5), 16α‐hydro‐17‐acetoxy‐ent‐kauran‐19‐oic acid (6), 16β‐hydro‐17‐hydroxy‐ent‐kauran‐19‐al (7), 16α‐hydro‐17‐hydroxy‐ent‐kauran‐19‐al (8), 16β‐hydro‐17‐hydroxy‐ent‐kauran‐19‐oic acid (9) and 16α‐hydro‐17‐hydroxy‐ent‐kauran‐19‐oic acid (10) were isolated from the stems of Annona cherimola. Among them, 1 is obtained for the first time from natural sources and 2 is a new compound. The structures of these compounds were characterized and identified by spectral analyses.
Studies with Polyfunctionally Substituted Heterocycles: New Synthesis of Pyrido[5′, 4′:2,3][1,3,4]oxadiazolo[3,2‐a]pyridine; Pyridazine; 1,3,4‐Oxadiazote; and Pyrazolo[1,5‐a]Pyrimidine Derivatives
Authors: Barsy Magda A. , Elmaghraby Mohammed A. , Ahmed Salwa M. 
Published: May 2015

AbstractCyanoacetohydrazide 1a reacts with 2‐arylhydrazoketons 2a,b and 3a,b in refluxing ethanol to yield pyrido[5′,4′:2,3][1,3,4]oxadiazolo[3,2‐a]pyridine and pyridazine derivatives; in the absence of solvent pyrazolo[1,5‐a]pyrimidine derivatives were obtained. The reaction of 2a,b and 3a,b will) benzoylhydrazine afforded 1,3,4‐oxadiazole and pyrazole derivatives.
Synthesis of 1‐(2‐Carboxyethylbenzyl)‐2‐benzenesulfonamidobicyclo[2.2.1]heptane: A Novel Potent Thromboxane Antagonist
Authors: Kan Wai‐Ming , Chem Ching‐Yuh , Su Sheng‐Fang 
Published: May 2015

AbstractA potent thromboxane antagonist, 1‐[2‐(2‐carboxyethyl)benzyl)]‐2‐benzenesulfonamidobicyclo‐[2.2.1]heptane was synthesized from norcamphor in 8 steps. It was shown to be a very potent thromboxane antagonist by inhibition of platelet aggregation induced by U46,619 at nanomolar concentration. The key intermediate 3‐[2‐bromomethylphenyl]propyl tetrahydropyran ether may be useful for the synthesis of other interphenylene containing prostaglandin analogs.
Effects of Ligand Chain Length and Terminal N‐Alkylation on the Protonation Constants and Stability Constants of Some Transition Metal Complexes of Linear Tetraaza and Pentaaza Ligands
Authors: Allen Chang C. , Shieh Fa‐Kun , Liu Yuh‐Liang , Chung Chung‐Sun 
Published: May 2015

AbstractA number of terminal N‐alkylated linear tetraaza and pentaaza ligands were prepared. Their ligand protonation constants and some transition and post‐transition metal (Ni2+, Cu2+, Zn2+, and Cd2+) complex stability constants were determined by potentiometric titration methods. In general, methylation and ethylation at the terminal nitrogen atoms caused the corresponding ligand nitrogen basicity to increase; however, the corresponding metal complex stabilities were decreased compared to the non‐alkylated structural analogs, presumably due to a steric effect.
Studies on 1, 2, 4‐Benzothiadiazine 1, 1‐Dioxide IX.1 Synthesis and Pharmacological Evaluation of 1, 2, 4‐Benzothiadiazine 1, 1‐Dioxide Biphenyl Tetrazoles as Angiotensin II Antagonists
Authors: Chem Ji‐Wang , Lin Hua‐Mei , Cheng Fong‐Chi , Lo Jir‐Chun , Lai Nan‐Yi , Kao Chai‐Lin , Usifoh Cyril O. 
Published: May 2015

AbstractIn the course of our investigations on the development of cardiovascular agents, 3‐butyl‐2‐[2′‐(2H‐tetrazol‐5‐yl)bipheny]‐4‐yl]methyl‐2H‐1, 2, 4‐benzothiadiazine 1, 1‐dioxide (2) was considered as a potential angiotensin II antagonist on the basis of bioisosteric replacement of the quinazoline ring of compound 1 with a 1, 2, 4‐benzothiadiazine 1, 1‐dioxide ring system. Alkylation of 6 with 4 afforded 7 and 8 in 24% and 28% yields, respectively. An attempt to remove the trityl group of compounds 7 and 8 under acidic condition gave the ring opened products 9 and 11 in 28% and 36% yields, respectively. However, compounds 2 and 10 were obtained in 46% and 85% yields when compounds 7 and 8 were refluxed in methanol. Preliminary assays of compounds 9 and 11 against angiotensin II receptors revealed weak activity with IC50 values of 3.6 μM and 5.4 μM, respectively. Compound 10 (IC50 = 87 nM) exhibited stronger binding affinity than compound 2 (IC50 = 750 nM).
Formal Synthesis of (±)‐Mitsugashiwalactone and (±)‐Isodihydronepetalactone from Norborn‐5‐en‐2‐one Involving Shapiro Reaction
Authors: Chang Meng‐Yang , Ciang Nein‐Chen 
Published: May 2015

AbstractA formal synthesis of (±)‐mitsugashiwalactone (1) and (±)‐isodihydro nepetalactone (2) was accomplished. Baeyer‐Villiger lactonization of ketone 9 followed by acidic treatment led to the rearranged lactone 8, which underwent a series of functional group transformations to give cyclopentanone derivatives 19 and 20. Shapiro reaction on 21 and 22 in the presence of excess dry ice gave lactones 5 and 6. Lactones 5 and 6 previously have been converted to mitsugashiwalactone (1) and isodihydronepetalactone (2), respectively.
Synthesis and Biological Activity of ω‐(5‐Aryl‐1,3,4‐oxadiazol‐2‐thio)‐ and ω‐(5‐Aryl‐1,3,4‐oxadiazol‐2‐thioacetoxyl)‐ω‐(1‐H‐1,2,4‐triazol‐1‐yl)acetophenones
Authors: Chu Chang‐Hu , Sun Xiao‐Wen , Sun Le , Zhang Zi‐Yi , Li Zhi‐Chun , Liao Ren‐An 
Published: May 2015

AbstractEighteen novel ω‐(5‐Aryl‐1,3.4‐oxadiazol‐2‐thio)‐ω‐(1‐H‐1,2,4‐triazol‐1‐yl)acetophenones (4a‐4i) and ω‐(5‐Aryl‐1,3,4‐oxadiazol‐2‐thioacetoxyl)‐ω‐(1‐H‐1,2,4‐triazol‐1‐yl)acetophenones (5a‐5i) were synthesized. All the compounds synthesized were confirmed by elemental analyses and spectral data. The biological activity of representative compounds was evaluated.
High Performance Liquid Chromatographic Enantioresolution of Benzoyl Amino Acid, Dipeptides and Tripeptide on β‐Cyclodextrin Bonded Stationary Phase Using Polar‐Organic Acetonitrile as the Mobile Phase
Authors: Chen Shushi 
Published: May 2015

AbstractThe enantiomeric resolution of several dipeptides, amino acid (i.e., isoleucine) and tripeptide (i.e., Leu‐Gly‐Phe) with two stereogenic centers on β‐cyclodextrin bonded chiral stationary phase (β‐CD CSP) using polar‐organic acetonitrile as the mobile phase is examined through pre‐column chemical derivatization with a series of tagging reagents such as benzoyl chloride, benzenesulfonyl chloride and 1‐naphthalenesulfonyl chloride. These tagging reagents are similar in structure; however, the enantioselectivity for the same analyte derivatized with these tagging reagents is quite different and found to be the best with benzoyl chloride. In the reversed‐phase mode or on the γ‐CD CSP under the same chromatographic conditions, the enantioresolution diminishes for all tagged enantiomers that were examined in this study. Dipeptides derivatized by benzoyl chloride appear to be better resolved than by dansyl chloride as reported previously. Interestingly, no enantioresolution for most derivatized amino acids with single stereogenic center was observed. Finally, enantioresolution can be enhanced by replacing the basic additive such as triethylamine with tripropylamine in the polar‐organic mobile phase.
Intramolecular Electron Transfer in CO‐Bound Mixed‐Valence Cytochrome c Oxidase Following CO Photolysis
Authors: He Qizhi , Pan Lian‐Ping , Chan Sunney I. 
Published: May 2015

AbstractInternal electron transfer in bovine cytochrome c oxidase was initiated by CO photolysis of the CO‐bound mixed‐valence form of the enzyme. Transient absorption spectroscopy was used to monitor changes in the redox states of the metal centers in the enzyme brought about by electron re‐equilibration. Upon CO photodissociation, reduced high spin cytochrome a3 was generated in less than 0.1 μsec, and a portion of the reduced cytochrome a3 was reoxidized with biphasic rate constants of k1 = 1.0 × 106 s−1 and k2 = 7.8 × 104 s−1. Concomitant reduction of cytochrome a was also observed with biphasic rate constants of k1 = 1.6 × 106 s−1 and k2 = 9 × 104 s−1. The stoichiometry of cytochrome a3 oxidized to cytochrome a reduced was found to be close to 1:1. Contrary to similar studies in the literature, no reduction of CuA was observed. As a control, no transient absorption changes corresponding to electron transfer was observed in the CO‐inhibited fully reduced form of the enzyme. These results indicate that there is significant electron reequilibration only between cytochrome a3 and cytochrome a upon photolysis of the CO‐bound mixed‐valence enzyme.
Biochemistry and Molecular Biology of Snake Neurotoxin
Authors: Yang Chen‐Chung , Chang Long‐Sen 
Published: May 2015

AbstractA review of research on snake neurotoxin is presented, with emphasis on the chemical modification studies and molecular cloning of postsynaptic and presynaptic neurotoxins from Naja naja atra (Taiwan cobra) (Fig. 1a) and Bungarus multicinctus (Taiwan banded krait) (Fig. 1b). Cobrotoxin and α‐bungarotoxin are the primary postsynaptic neurotoxins isolated from the venom of N. naja atra and B. multicinctus, respectively. Although they share a common three‐dimensional structure, the functional elements essential for the manifestation of their toxicity are different. Selective and stepwise chemical modification of cobrotoxin indicate that at least two cationic groups, an ϵ‐amino group of Lys‐47 and a guanidino group of Arg‐33 common to all known postsynaptic neurotoxins, are functionally important for its neuromuscular blocking activity. However, for α‐bungarotoxin, the side chains of several basic amino acid residues are involved in the multipoint contact between the toxin and acetylcholine receptor. Moreover, the conserved Trp residue is essential for the neurotoxicity of cobrotoxin, but not for α‐bungarotoxin. The cDNAs encoding cobrotoxin and α‐bungarotoxin was constructed from the cellular RNA isolated from the venom glands of N. naja atra and B. multicinctus by polymerase chain reaction. The sequence of their 3′‐untranslational region, signal peptide and 5′‐untranslational region share a high degree of homology, suggesting that they are evolutionarily related. Expression of both neurotoxic protein in E. coli generated polypeptide chains for reactions with the antisera against the native neurotoxins.Presynaptic neurotoxins constitute a different group of neurotoxic proteins in snake venom proteins. These presynaptic neurotoxins are either basic phospholipase A2 (PLA2) per se or contain basic PLA2 as an indispensible part of their structures. Thus, the presynaptic neurotoxins usually show both PLA2 activity and presynaptic neurotoxicity. β‐Bungarotoxin (β‐Bgt), the main presynaptic PLA2 neurotoxin isolated from the venom of B. multicinctus (Taiwan banded krait), consists of two dissimilar polypeptide chains, a PLA2 subunit (A chain) and potassium channel recognition subunit (B chain). Chemical modification studies show that the toxin might possess two functional sites, one responsible for the catalytic activity and the other for its pharmacological properties. Molecular cloning and expression of the A chain and B chain of β‐Bgt reveal that the A chain of β‐Bgt is an active subunit with PLA2 activity, and that the B chain is involved in voltage‐gated potassium channel blocking action observed with β‐Bgt.
Recent New Developments of Steady‐State and Time‐Dependent Density Functional Theories for the Treatment of Structure and Dynamics of Many‐Electron Atomic, Molecular, and Quantum Dot Systems
Authors: Chu Shih‐I , Tong Xiao‐Min , Chu Xi , Telnov Dmitry 
Published: May 2015

AbstractWe present a short account of recent new developments of density‐functional theory (DFT) for accurate and efficient treatments of the electronic structure and quantum dynamics of many‐electron systems. The conventional DFT calculations contain spurious self‐interaction energy and improper long‐range potential, preventing reliable description of the excited and resonance states. We present a new DFT with optimized effective potential (OEP) and self‐interaction‐correction (SIC) to overcome some of the major difficulties encountered in conventional DFT treatments using explicit energy functionals. The OEP‐SIC formalism uses only orbital‐independent single‐particle local potentials and is self‐interaction free, providing a theoretical framework for accurate description of the excited‐state properties and quantum dynamics. Several applications of the new procedure are presented, including: (a) the first successful DFT treatment of the atomic autoionizing resonances, (b) a relativistic extension of the OEP‐SIC formalism for the calculation of the atomic structure with results in good agreement with the experimental data across the periodic table (Z = 2–106), (c) electronic structure calculation of the ionization properties of molecules, and (d) the delicated “shell‐filling” electronic structure in quantum dots. Finally we present also new formulations of time‐dependent DFT for nonperturbative treatment of atomic and molecular multiphoton and nonlinear optical processes in intense and superintense laser fields. Both the time‐independent Floquet approach and the time‐dependent OEP‐SIC technique are introduced. Application of the time‐dependent DFT/OEP‐SIC procedure to the study of multiple high‐order harmonic generation processes in intense ultrashort pulsed laser fields is discussed in detail.
Metal String Complexes: Synthesis, Crystal Structures and Magnetic Properties of Heptanuclear Nickel(II) Complexes, [Ni7(μ7‐teptra)4X2] (teptra = tetrapyridyltriamido, X = Cl−, NCS−)
Authors: Lai Shie‐Yang , Wang Chih‐Chieh , Chen Yu‐Hua , Lee Chung‐Chou , Liu Yi‐Hung , Peng Shie‐Ming 
Published: May 2015

AbstractThe synthesis, crystal structures and magnetic properties of linear heptanuclear nickel(II) complexes [Ni7(μ7‐teptra)4X2], (teptra = tetrapyridyltriamido), with the axial ligand X = Cl− (1), NCS− (2), are reported. The hepta‐nuclear metal chain is helically wrapped by four syn‐syn‐syn‐syn‐syn‐syn teptra3− ligands. Both of the [Ni7(μ7‐teptra)4]2+ moiety are isostructural involving a Ni7 linear chain unit with all of the ∠Ni‐Ni‐Ni being 180°, terminated by two axial ligands. Three types of NiNi distances are found in these complexes. The longest ones bonded with the axial ligands are 2.383(1), 2.374(2) and 2.375(2), 2.354(2), and the intermediate ones are 2.310(1), 2.304(1) and 2.300(2), 2.303(2) Å for (1) and (2), respectively. The innermost NiNi distances are the shortest ones with the distances of 2.226(2), 2.214(2) and 2.194(2), 2.206(2) Å for (1) and (2), respectively. Two terminal Ni(II) ions bonded with the axial ligands are in a square‐pyramidal (NiN4X) environment and exhibit long NiN bonds (−2.10 Å) which are consistent with a high‐spin Ni(II) configuration. The inner five Ni(II) ions displayed short NiN (∼1.90 Å) bond distances which are consistent with a square‐planar (NiN4), diamagnetic arrangement of a low‐spin Ni(II) configuration. The magnetic measurement of (1) shows an antiferromagnetic interaction of two terminal high‐spin Ni(II) ions with the coupling constant J = −3.8 cm−1. The NiNi, NiN distances and magnetic behavior among tri‐, penta‐, and hepta‐nickel(II) complexes are compared and discussed.
Synthesis of Two N,N′‐bis(N,N‐dialkylamide) Derivatives of Diethylenetriaminepentaacetic Acid and the Stabilities of Their Complexes with Gd3+, Ca2+, Cu2+, and Zn2+
Authors: Wang Yun‐Ming , Wang Yueh‐Ju , Liu Gin‐Chung , Kuo Yu‐Ting 
Published: May 2015

AbstractTwo bis(N,N‐dialkylamide) derivatives of DTPA [(carboxymethyl)iminobis (ethylenenitrilo) tetraacetic acid], DTPA‐BDMA = the bis(N,N‐dimethylamide) and DTPA‐BDEA = the bis(N,N‐diethylamide) were synthesized. Their protonation constants were determined by potentiometric titration in 0.10 M Me4NNO3 and by NMR pH titration at 25.0 ± 0.1 °C. Stability and selectivity constants were measured to evaluate the possibility of using the corresponding gadolinium(III) complexes for magnetic resonance imaging contrast agents. The stability constants of gadolinium(III), copper(II), zinc(II), and calcium(II) complexes with DTPA‐BDMA and DTPA‐BDEA were investigated quantitatively by potentiometry. The stability constant for gadolinium(III) complexes is larger than those for Ca(II), Zn(II), and Cu(II) complexes. The selectivity constants and modified selectivity constants of the amides for Gd3+ over endogenously available metal ions were calculated. Effectiveness of these two ligands in binding divalent and trivalent metal ions in biological media is assessed by comparing pM values at physiological pH 7.4. Spin‐lattice relaxivity values R1 for Gd(III) complexes were also determined. The observed relaxivity values were found to decrease with increasing pH in the acid range below pH 4 and relaxivity values became invariant with respect to pH changes over the range of 4–10. 17O NMR shifts showed that the [Dy(DTPA‐BDMA)] and [Dy(DTPA‐BDEA)] complexes had one inner‐sphere water molecule. Water proton spin‐lattice relaxation rates for the [Gd(DTPA‐BDMA)] and [Gd(DTPA‐BDEA)] complexes were also consistent with one inner‐sphere gadolinium(III) coordination position.
First Total Synthesis of (±)‐13‐Hydroxyneocembrene
Authors: Xing Yacheng , Cen Wen , Lan Jiong , Li Ying , Li Yulin 
Published: May 2015

AbstractFirst total synthesis of (±)‐13‐hydroxyneocembrene (1), starting from 6‐methyl‐5‐hepten‐2‐one (6) and geraniol (7), is described. The key steps are (i) the addition of sulfur‐stabilized carbanion 12 to aldehyde 9, (ii) the synthesis of 18 by using phase‐transfer catalyzed coupling reaction, and (iii) low‐valent titanium‐induced intramolecular coupling of oxo aldehyde 3 to afford the target molecule after the final deprotection.
A Sensitive Colorimetric Method for the Determination of Arsenic in Environmental and Biological Samples
Authors: Agrawal Omi , Sunita G. , Gupta V. K. 
Published: May 2015

AbstractResults of a thorough study and application of leucocrystal violet for the determination of arsenic in parts per million (ppm) levels in environmental and biological samples is described here. The proposed method is based on the reaction of arsenic with potassium iodate to liberate iodine. The liberated iodine selectively oxidises leucocrystal violet to form crystal violet dye in the presence of sodium hydroxide. The dye formed shows maximum absorbance at 592 nm. The detection limit of arsenic is 0.002 μgmL−1 and the method obeys Beer's law over the concentration range of 0.1 μg ‐ 1.0 μg of per 25 mL of final solution (0.004–0.04 ppm). The molar absorptivity was found to be 1.49 × 106 L mol−1 cm−1. The proposed method was successfully applied for the determination of arsenic in various environmental and biological samples. The results are in good agreement with the standard reported method.
A Theoretical Study of Photocurrent in Conjugated Polymer Systems
Authors: Chang R. , Lin S. H. 
Published: May 2015

AbstractWe present a theory of photocurrent spectra for a light‐emitting diode fabricated by a π‐conjugated polymer system. The tunneling through a barrier induced by the DC field dominates the dissociation of excitor states (i.e., photoionization of vibronic states) below the threshold. The spectra thus obtained explain most of the experimental results and also allows us to more easily position the S2 state and other higher states in the conjugated polymer.
Systematic NMR Assignments of DNA Exchangeable Protons
Authors: Tseng Yu‐Yu , Chou Shan‐Ho 
Published: May 2015

AbstractExchangeable proton information is of critical importance in the NMR structural studies of DNA/RNA oligomers and/or their complexes with protein. However, no systematic NMR assignment strategy about these protons has been reported until now, although similar studies about non‐exchangeable proton assignment have been well established in the past decade. We now report detailed NMR studies of a DNA duplex (d20‐mer) containing the recognition site (5′‐ATGAC‐3′) of GCN4 basic zinc finger protein. All exchangeable and non‐exchangeable protons except H5′/H5″ protons could be assigned from a single H2O/NOESY spectrum at 25°C. The assignment strategy reported in this paper shall be useful to NMR studies of unusual DNA/RNA structures and their complexes with peptides/proteins.
Purification and Characterization of a Cephalexin‐Synthesizing Enzyme from Gluconobacter oxydans CCRC10383
Authors: Yang Fang‐Lin , Wu Shih‐Hsiung 
Published: May 2015

AbstractA cephalexin‐synthesizing enzyme which catalyzes the synthesis of cephalexin from two substrates, 7‐amino‐3‐deacetoxy cephalosporanic acid (7‐ADCA) and D‐phenylglycine methyl ester (D‐MEPG) was isolated, purified and characterized from G. oxydans CCRC 10383 by ammonium sulfate precipitation, CM‐Fractogel and Sephadex G‐200 chromatography. The molecular weight of the enzyme was estimated to be 270 kd by gel filtration. From the analysis of SDS‐PAGE, the enzyme is a tetramer and consists of two 53 kd subunits and two 73 kd subunits. The isoelectric point of the enzyme was estimated to be 7.5. The optimal pH and temperature for the synthetic reaction of cephalexin were 5–7 and 30–50°C, respectively, with a maxium reaction rate at pH 6 or 50°C. Metal ions are not essential for the enzymatic activity because EDTA (ethylenediaminetetraacetic acid) exerts no influence upon the enzyme activity. The growth medium containing 0.25% DL‐phenylglycine (DL‐PG) or 0.25% D‐phenylglycine (D‐PG) as inducers could obtain 1.4 times higher enzyme activity than the growth medium without inducers. The values of Km, Kcat, Vmax and bimolecular constant Kcat/Km were 19 mM, 1.2 × 104 s−1, 30 unit/mg of protein and 6.2 × 105 M−1s−1, respectively. The Km values for D‐MEPG and 7‐ADCA were determined as 13.9 mM and 3.08 mM, respectively. The conversion of cephalexin was found to be 60% when the synthesis was carried out in the 0.1 M phosphate buffer solution (pH 6.2) containing 40 mM of 7‐ADCA and 118 mM of MEPG at 37°C.
Design and Synthesis of an Activity Probe for Protein Tyrosine Phosphatases
Authors: Lo Lee‐Chiang , Wang Hsin‐Yi , Wang Zi‐Jien 
Published: May 2015

AbstractProtein tyrosine phosphatases (PTPases) are an important class of enzymes involved in the regulation of many cellular events. Here we describe the design and synthesis of an activity probe 2 targeting these PTPases. This mechanism‐based activity probe adopts a cassette‐like design; a phosphate group serves as the recognition head and a fluorescent diethylaminocoumarin derivative acts as the reporter group. Compound 3 was phosphorylated with diallyl phosphorochloridate and then fluorinated with DAST to give versatile intermediate 5. The Boc protective group of compound 5 was removed by TEA to make available the amino group where a diethylaminocoumarin chromophore was later attached. Final deprotection of the allyl group from the phosphate head gives our complete activity probe 2. It will be used in the labeling study of PTPases from various sources.
Expression, Purification, and Characterization of the Infectious Bursal Disease Virus‐Like Particles Produced by Insect Cells
Authors: Ho Jin‐Yi , Lai Su‐Yuan , Lee Long‐Huw , Wang Min‐Ying 
Published: May 2015

AbstractThe cDNA of the large genomic segment A (ORF A1) of infectious bursal disease virus (IBDV) strain P3009 was synthesized and cloned into the transfer vector pBlueBac4. Following cotransfection and plaque purification, a plaque‐pure recombinant baculovirus, vIBDVJ6, was selected to express IBDV capsid proteins. When Sf9 cells were infected with vIBDVJ6, IBDV precursor protein (polypeptide of VP2‐VP4‐VP3) was expressed and proteolytically processed to give VP2, VP3, and VP4 proteins by a viral protease. These resulting recombinant proteins can self‐assemble to form virus‐like particles (VLPs). The shape and size of the negatively stained purified IBDV‐like particles were demonstrated to be similar to those of the complete P3009 IBDV particles under direct electron microscopic observation. Furthermore, the purified particles were strongly recognized by an anti‐VP2 monoclonal antibody as confirmed by an immuno‐gold labeling experiment. IBDV‐like particles will offer a unique opportunity to create completely non‐infectious viral vaccines for use in the vaccination of chickens. The immunologic response in chickens stimulated by IBDV‐like particles is currently being evaluated.