bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dementia with Lewy bodies and gait neural basis: a cross-sectional study
Authors: Sainsily-Cesarus Adele , Schmitt Elise , Landre Lionel , Botzung Anne , Rauch Lucie , Demuynck Catherine , Philippi Nathalie , de Sousa Paulo Loureiro , Mutter Catherine , Cretin Benjamin , Martin-Hunyadi Catherine , Blanc Frederic 
Published: July 2024

Abstract
Background
Dementia with Lewy Bodies (DLB) is responsible for cognitive-behavioural disorders but also for gait disorders. The latter are thought to be related to parkinsonism, but the neural bases of these disorders are not well known, especially in the early stages. The aim of this study was to investigate by volumetric Magnetic Resonance Imaging the neuronal basis of gait disorders in DLB patients, compared to Healthy Elderly Controls and Alzheimer’s Disease patients.

Methods
Clinical examination with motor assessment including 10-meter walking speed, one-leg balance and Timed Up and Go test, a comprehensive neuropsychological evaluation and 3D brain Magnetic Resonance Imaging were performed on 84 DLB patients, 39 Alzheimer’s Disease patients and 22 Healthy Elderly Controls. We used Statistical Parametric Mapping 12 to perform a one-sample t-test to investigate the correlation between each gait score and gray matter volume (P ≤ 0.05 corrected for family-wise error).

Results
We found a correlation for DLB patients between walking speed and gray matter decrease (P < 0.05, corrected for family-wise error) in caudate nuclei, anterior cingulate cortex, mid-cingulate cortex, hippocampi, supplementary motor area, right cerebellar cortex and left parietal operculum. We found no correlation with Timed Up and Go test and one-leg balance.

Conclusion
Gait disorders are underpinned by certain classical regions such as the cerebellum and the supplementary motor area. Our results suggest there may be a motivational and emotional component of voluntary gait in DLB subjects, underpinned by the cingulate cortex, a spatial orientation component, underpinned by hippocampi and suggest the involvement of brain processing speed and parkinsonism, underpinned by the caudate nuclei.

Trial registration
The study protocol has been registered on ClinicalTrials.gov. (NCT01876459) on June 12, 2013.
Arthroscopic reduction and internal fixation for fracture of the posterior process of the talus (Shepherd’s fracture): a case report
Authors: Krivokapic Branislav , DHooghe Pieter , Bogosavljevic Nikola , Jeremic Danilo , Rajović Nina 
Published: July 2024

Abstract
Introduction
Fracture of the lateral tubercle of the posterior process of the talus (Shepherd fracture) is an uncommon injury seen in sport. It is secondary either to indirect trauma on the plantarflexed foot or to high-impact direct trauma. The fracture can be missed with conventional X-rays and therefore advanced imaging methods such as CT scans are usually warranted for management planning. There is a low threshold towards surgical management in the displaced or comminuted case as the delayed functional outcome with conservative treatment is frequently sub-optimal with long-term pain, degenerative changes and non-union. In this regard, recent years saw an increasing interest in the role of minimally invasive approaches for Shepherd´s fracture treatment, such as arthroscopic reduction and internal fixation (ARIF).

Case report
We present a case of a 27-year-old white male professional football player from Serbia who had Shepard fracture and successfully managed with arthroscopic osteosynthesis. The technical approach is detailed with posterior ankle arthroscopy offering the advantages of a minimally invasive approach with low morbidity and a rapid return to regular sporting activities.

Conclusion
The utilization of the 2-port arthroscopic approach this method enables the direct observation of the articular surface along with the corresponding fracture lines, thereby affording the surgeon the chance to achieve accurate reduction via a minimally invasive soft tissue aperture. We advocate that Arthroscopic reduction and internal fixation (ARIF) is a reliable method for the fixation of Shepherd's fracture in the hands of experienced ankle arthroscopists.
Characteristic of phenotype, amino acids and volatile compounds for fresh tea leaves of Korean tea cultivars (Camellia sinensis (L.) O. Kuntze)
Authors: Kang Suyoung , Lee So Jin , Kwon YongHee , Moon Doo-Gyung , Sun Jung Hun , Hwang Kyu-Won , Moon Joon-Kwan 
Published: July 2024

AbstractTea (Camellia sinensis (L.) O. Kuntze) is a popular beverage consumed worldwide. To establish fundamental scientific data, we analyzed the amino acids and volatile compounds in seven tea cultivars grown in Korea investigated phenotype also. Phenotypically, the leaf area and greenness index of young shoots and leaf blades were particularly different among the four Korean cultivars. Nine amino acids were detected from each cultivar, with the total amino acid and theanine contents being 9.08–41.42 and 2.81–24.60 mg/g, respectively. Moreover, 107 volatile compounds were identified as common components among tea cultivars using headspace solid-phase microextraction / gas chromatography–mass spectrometry (HS-SPME/GC-MS), and 38 key compounds were identified using partial least squares-discriminant analysis (PLS-DA) and hierarchical cluster analysis (HCA). The (Z)-linalool oxide (furanoid) concentrations were significantly high in Korean tea plant cultivars, and linalool concentrations were also high or low, but had high relative contents. Linalool and its various oxides are the major compounds responsible for the tea aroma. In conclusion, Korean tea cultivars have distinct characteristics, and the results of this study will form the basis for identifying Korean tea plant cultivars that can produce high-value tea products.
CD71 + erythroid cells promote intestinal symbiotic microbial communities in pregnancy and neonatal period
Authors: Koleva Petya , He Jia , Dunsmore Garett , Bozorgmehr Najmeh , Lu Julia , Huynh Maia , Tollenaar Stephanie , Huang Vivian , Walter Jens , Way Sing Sing , Elahi Shokrollah 
Published: July 2024

Abstract
Background
The establishment of microbial communities in neonatal mammals plays a pivotal role in shaping their immune responses to infections and other immune-related conditions. This process is influenced by a combination of endogenous and exogenous factors. Previously, we reported that depletion of CD71 + erythroid cells (CECs) results in an inflammatory response to microbial communities in newborn mice.

Results
Here, we systemically tested this hypothesis and observed that the small intestinal lamina propria of neonatal mice had the highest frequency of CECs during the early days of life. This high abundance of CECs was attributed to erythropoiesis niches within the small intestinal tissues. Notably, the removal of CECs from the intestinal tissues by the anti-CD71 antibody disrupted immune homeostasis. This disruption was evident by alteration in the expression of antimicrobial peptides (AMPs), toll-like receptors (TLRs), inflammatory cytokines/chemokines, and resulting in microbial dysbiosis. Intriguingly, these alterations in microbial communities persisted when tested 5 weeks post-treatment, with a more notable effect observed in female mice. This illustrates a sex-dependent association between CECs and neonatal microbiome modulation. Moreover, we extended our studies on pregnant mice, observing that modulating CECs substantially alters the frequency and diversity of their microbial communities. Finally, we found a significantly lower proportion of CECs in the cord blood of pre-term human newborns, suggesting a potential role in dysregulated immune responses to microbial communities in the gut.

Conclusions
Our findings provide novel insights into pivotal role of CECs in immune homeostasis and swift adaptation of microbial communities in newborns. Despite the complexity of the cellular biology of the gut, our findings shed light on the previously unappreciated role of CECs in the dialogue between the microbiota and immune system. These findings have significant implications for human health.
Compromises in occupational choice and premature termination of vocational education and training: gender type, prestige, and occupational interests in focus
Authors: Fischer-Browne Melanie , Ahrens Lea , Kleinert Corinna , Schels Brigitte 
Published: July 2024

AbstractThis study examines whether premature termination of vocational education and training (VET) is more likely when trainees have to make compromises between their realistic occupational aspirations and the occupation for which they are trained in terms of the occupational dimensions of gender type, prestige, and occupational interests. Specifically, it examines the relative importance of the three compromise dimensions on premature VET termination. The study is based on a sample of 3,241 VET entrants from a cohort of ninth graders in 2010 in the German National Educational Panel Study (NEPS-SC4) in combination with aggregated data on occupational characteristics. Results from discrete hazard rate models indicate a higher risk of premature VET termination for trainees whose VET occupation differs from their realistic occupational aspiration from their school days. While compromises in prestige have no overall effect, both compromises in interests and gender type threaten the stability of VET. The results also indicate that compromises in interests have the strongest effect on the risk of premature VET termination and that the effects of compromises in gender type are partly mediated by the effects of compromises in interests. Furthermore, sensitivity analysis revealed that the main effects of compromises in gender type and prestige are gender specific.
From pilot to practice: navigating pre-requisites for up-scaling sustainable urban solutions
Authors: Sondal Jonas , Hellquist Alexander , Balfors Berit 
Published: July 2024

AbstractMunicipalities employ pilot projects, tests, living labs and similar initiatives to explore novel sustainable solutions for urban development. Nevertheless, civil servants often encounter challenges when attempting to scale up tested solutions, both within their own city and to other cities. To address this and increase knowledge on how theory can be utilised by practitioners, this research project has created a supportive tool aimed at facilitating the upscaling process. The development took place in co-creation with civil servants from the three largest cities in Sweden. The paper confirms earlier research on the need of institutional capacity as well as appropriate navigation between institutional logics as pre-requisites for upscaling. More specifically, institutional capacity plays a vital role for securing sufficient resources and mandates for upscaling, extending beyond mere dissemination of results. The supportive tool aims at enhancing this institutional capacity and translating between institutional logics. In addition, the paper contributes to the scientific debate on civil servants’ understanding of different forms of upscaling and, consequently, the need to articulate these differences when facilitating upscaling. The paper emphasizes that upscaling should be conceptualised not as a discrete event but rather an ongoing process starting already when planning a pilot project.
Association of host antimicrobial peptides with type II diabetes mellitus complications: a systematic review
Authors: Abdul Kareem Hawraa Ibrahim , Mohammed Suhad Hadi 
Published: July 2024

Abstract
Background
This systematic review aims to review and assess the importance and relationship between host defence antimicrobial peptides with type 2 diabetes mellitus (T2DM) complications and the correlation of their expression with hyperglycaemic status.

Main body
The systematic search included three electronic databases (PMC, PubMed, and Google Scholar) that were searched from July to November 2023. After identifying and screening the research articles, eleven studies fulfilled the selection criteria and were included (six case–control and five cross-sectional studies). The Newcastle Ottawa Scale assessed the selected studies’ quality. Most studies indicated a correlation between certain types of AMPs and diabetic complications.

Conclusion
Hyperglycaemia in type 2 diabetes mellitus (T2DM) affects the expression of certain types of antimicrobial peptides (AMPs) which have a dual function (antibacterial and modulation of immune response) that may enhance inflammation which may correlate with the development of long-term complications, increased susceptibility to bacterial infection, and impaired wound healing.
A fast fish swimming protocol that provides similar insights to critical swimming speed
Authors: Bamford Stephanie M. , Seebacher Frank 
Published: July 2024

ABSTRACT
Performance measures are an important tool to assess the impact of environmental change on animals. In fish, performance is often measured as critical swimming speed (Ucrit), which reflects individual maximal physiological capacities. A drawback of Ucrit is that trials are relatively long (∼30-75 min). Ucrit may therefore not be suitable for several repeated measurements because of the potential for training effects, long recovery periods, and low throughput. Here we test a shorter (∼4-5 min) protocol, “Ucrit fast” (UCfast) in zebrafish (Danio rerio). We show that UCfast and Ucrit have similar, significant repeatabilities within individuals. Unlike Ucrit, repeated UCfast trials did not elicit a training effect. Both UCfast and Ucrit provide the same insights into thermal acclimation, and both provide similar estimates of individual acclimation capacity in doubly acclimated fish. We propose that UCfast is a valid substitute for Ucrit particularly when higher throughput and several repeated measures are necessary.
Reflections on alma mater: Origin and evolution of Ruhr University Bochum
Authors: Ahmad HR , Schläfke Mariane 
Published: July 2024

doi: https://doi.org/10.12669/pjms.40.8.10111
How to cite this: Ahmad HR, Schläfke M. Reflections on alma mater: Origin and evolution of Ruhr University Bochum. Pak J Med Sci. 2024;40(8):---------. doi: https://doi.org/10.12669/pjms.40.8.10111
Analysis of blood microbiota in patients with adult-onset Still’s disease and sepsis by metagenomic next-generation sequencing
Authors: Fang Quanquan , Xie Jingzhi , Yin Songlou , Yin Hanqiu , Zhou Dongmei 
Published: July 2024

Aims/Background Adult-onset Still’s disease (AOSD) shares similar clinical symptoms with sepsis. Thus, differentiating between AOSD and sepsis presents a great challenge while making diagnosis. This study aimed to analyse the changes in blood microbiota related to AOSD and sepsis using metagenomic next-generation sequencing (mNGS), identify potential biomarkers that distinguish AOSD from sepsis, and explore the diagnostic value of mNGS in differentiation between these two pathological conditions. Methods Clinical data of four AOSD patients and four sepsis patients treated in the Department of Rheumatology and Immunology, The Affiliated Hospital of Xuzhou Medical University between October 2021 and February 2022 were collected. The mNGS diagnostic records of these patients were analysed for microbial correlations in terms of species taxonomic structure and beta diversity by comparing blood microbiota between AOSD and sepsis. The biomarkers with the strongest capability in distinguishing the subgroups were screened using a random forest algorithm. Results There was no statistically significant differences between AOSD patients and sepsis controls in terms of gender and age (p > 0.05). A total of 91 operational taxonomic units (OTUs) were obtained. At the level of phylum, Proteobacteria, Ascomycota and Basidiomycota were present in high abundances in both groups (79.76%, 14.18% and 3.30% vs 54.03%, 32.77% and 5.81%). At the genus level, the abundances of Parainfluenzae, Aspergillus and Ralstonia were the top three highest in the AOSD group (73.88%, 10.92% and 5.48%), while Ralstonia, Aspergillus and Malassezia were ranked as the top three in the sepsis group in term of abundance (48.69%, 27.36% and 5.52%). In beta-diversity analysis, there were advances shown in visual principal coordinates analysis (PCoA) and non-metric multidimensional scaling (NMDS) between the AOSD group and sepsis group (p < 0.05), with little significant differences in the analysis of similarities (Anosim) (p > 0.05). Linear discriminant analysis effect size (LEfSe) showed that Mucoromycota, Saccharomycetes, Moraxellales, Mucorales, Xanthomonadales, Saccharomycetales, Acinetobacter, Stenotrophomonas, Yarrowia, Apophysomyces, Acinetobacter johnson, Yarrowia lipolytica, Apophysomyces variabilis and Stenotrophomonas maltophilia were more enriched in sepsis group (p < 0.05). The top five variables with the strongest capability in distinguishing between AOSD and sepsis were Acinetobacter johnsonii, Apophysomyces variabilis, Propionibacterium acnes, Stenotrophomonas maltophilia and Yarrowia lipolytica. Conclusion The blood microorganisms in AOSD were different from sepsis, and mNGS was potential to distinguish between AOSD and sepsis.
Noninvasive high-frequency oscillation ventilation as post- extubation respiratory support in neonates: Systematic review and meta-analysis
Authors: Prasad Rameshwar , Saha Bijan , Sk Md Habibullah , Sahoo Jagdish Prasad , Gupta Bhupendra Kumar , Shaw Subhash Chandra 
Published: July 2024

IntroductionNoninvasive High-Frequency Oscillatory Ventilation (NHFOV) is increasingly being adopted to reduce the need for invasive ventilation after extubation.ObjectivesTo evaluate the benefits and harms of NHFOV as post-extubation respiratory support in newborns compared to other non‐invasive respiratory support modes.Material & methodsWe included randomized controlled trials comparing NHFOV with other non-invasive modes post-extubation in newborns. Data sources were MEDLINE (via Pubmed), Cochrane Central Register of Controlled Trials, China National Knowledge Infrastructure, WHO international clinical trials registry platform and Clinical Trial Registry, forward and backward citation search. Methodological quality of studies was assessed by Cochrane’s Risk of Bias tool 1.0.ResultsThis systematic review included 21 studies and 3294 participants, the majority of whom were preterm. NHFOV compared to nasal continuous positive airway pressure (NCPAP) reduced reintubation within seven days (RR 0.34, 95% CI 0.22 to 0.53) after extubation. It also reduced extubation failure (RR 0.39, 95% CI 0.30 to 0.51) and reintubation within 72 hrs (RR 0.40, 95% CI 0.31 to 0.53), bronchopulmonary dysplasia (RR 0.59, 95% CI 0.37 to 0.94) and pulmonary air leak (RR 0.46, 95% CI 0.27 to 0.79) compared to NCPAP. The rate of reintubation within seven days (RR 0.62, 95% CI 0.18 to 2.14) was similar whereas extubation failure (RR 0.65, 95% CI 0.50 to 0.83) and reintubation (RR 0.68, 95% CI 0.52 to 0.89) within 72 hrs were lower in NHFOV group compared to nasal intermittent positive pressure ventilation. There was no effect on other outcomes. Overall quality of the evidence was low to very low in both comparisons.ConclusionsNHFOV may reduce the rate of reintubation and extubation failure post-extubation without increasing complications. Majority of the trials were exclusively done in preterm neonates. Further research with high methodological quality is warranted.
Projections of Passing: Postwar Anxieties and Hollywood Films, 1947–1960, N. Megan Kelley (2016)
Authors: Elana Holland 
Published: July 2024

Review of: Projections of Passing: Postwar Anxieties and Hollywood Films, 1947–1960, N. Megan Kelley (2016)
Jackson: University Press of Mississippi, 288pp., ISBN: 9781496834546 (pbk), $30.00, ISBN: 9781496806277 (hbk), $110.00
Evaluation of the activity of antimicrobial peptides against bacterial vaginosis
Authors: Kang Xuning , Zhao Ting , Song Yuzhu , Zhang Jinyang , Yuan Tao , Han Qinqin 
Published: July 2024

Abstract
New drugs for the treatment of bacterial vaginosis (BV) are yet to be developed due to concerns that they may contribute to the increase in antibiotic resistance in BV. Antimicrobial peptides (AMPs) are one of the most promising options for next-generation antibiotics. In this study, we investigated the bacteriostatic activity of the AMPs Pexiganan, plectasin, melittin, and cathelicidin-DM against Gram-negative and Gram-positive bacteria both in vitro and in a mouse model of BV infection. The results showed that Pexiganan, melittin, and cathelicidin-DM had significant antibacterial activity against both Gram-negative and Gram-positive bacteria. AMPs have great potential for clinical application in the treatment of vaginitis, and this study provides an experimental basis for their use in the active immunoprophylaxis of BV.
Muga (Antheraea assamensis) silk electrospun scaffold for biomedical applications
Authors: Sundaramoorthy Subramanian , Kumar Balan Karthic , Ramaiyan Alwar Saravanan , Caraline I. , Gowri S. , Kavitha S. 
Published: July 2024

Abstract
Wild silk varieties are less explored in the field of biomaterials than cultivated mulberry silk (Bombyx Mori). In the present work, an electrospun scaffold was produced from wild silk, muga (Antheraea assamensis), and it was assessed for the properties required for biomaterials. Scanning electron microscopy images showed that the electrospun fibers were formed without beads, and the majority of the fibers had diameters in the range of 500–900 nm. The scaffold started to decompose at 210 °C, which is higher than the temperature used for sterilization of the scaffold. The PBS uptake percentage was found to be 400 %, which is sufficient for the absorption of the medium used during cell culture. The scaffold had a hemolysis value of less than 5 %, which indicates that the scaffold exhibits good blood compatibility. The MTT assay showed cell viability of more than 80 % after 24 h and 48 h, and fluorescence microscopy showed that cells adhered and spread on the surface of the scaffold. The results show that muga silk electrospun mat can be used as an ideal biomaterial.
Crystal structure of (S)-4-(2-(4-(2-acetyl-5-chlorophenyl)-3-methoxy-6-oxopyridazin-1(6H)-yl)-3-phenylpropanamido)benzoic acid monohydrate, C29H26ClN3O7
Authors: Wen-Jie Xu , Ying Xiao , Duan-Ming Tan , Song Li 
Published: July 2024

Abstract
C29H26ClN3O7, monoclinic, P21 (no. 4), a = 15.5488(6) Å, b = 7.9366(3) Å, c = 22.6498(9) Å, β = 93.006(2)°, V = 2,791.24(19) Å3, Z = 4, R

gt
(F) = 0.0528, wR

ref
(F
2) = 0.1391, T = 292 K.