bluuebunny/crossref_metadata_2025_split huggingface dataset:
Structural characterization of iron azadiethylthiolate complexes: Insights from NMR, crystallography, and ion mobility‐mass spectroscopy
Authors: Liu Yu‐Chiao , Ho Yi‐Chi , Lee Gene‐Hsiang , Chiang Ming‐Hsi , Tseng Mei‐Chun 
Published: July 2024

AbstractFe3(CO)12 reacted with azadiethylthiols afforded [Fe2(μ‐S(CH2)2NR(CH2)2S)(CO)6]2 (R = nPr, 1A; iPr, 2A). Structure analysis revealed that 1A features the anti‐(a,e,a,e) configuration, while its iso‐propyl 2A adopts the syn‐(a,e,a,e) arrangement. NMR measurements at room temperature confirmed this structure, showing distinct signals for 2 NCH2 and 2 SCH2 methylene groups. Acid treatment of 1–2 yielded N‐protonated species (1A–H, 2A–H), maintaining the anti‐(a,e,a,e) configuration. Isolation of 1B–H revealed both the syn‐(a,e,e,a) and anti‐(a,e,e,a) configuration by X‐ray single‐crystallography. Protonation of 1A–H induced downfield shifts in methylene proton signals, with NH hydrogen recorded at 6.96 ppm. Furthermore, 1B–H displays similar NMR behavior, indicating the coexistence of two isomeric molecules of 1B–H, syn‐(a,e,e,a) and anti‐(a,e,e,a), which is consistent with the crystallographic results. Moreover, ion mobility‐mass spectrometry (IM‐MS) is harnessed for the swift identification and differentiation of these isomeric complexes in solution. By leveraging accurate mass measurements and MS/MS analyses, the molecular formula and constituents are validated. The sample mobility enables straightforward characterization of 1A, 1B, and 2A in relation to their stereo‐ and regio‐configurations, obviating the need for extensive time or sample quantities. Integration of findings from X‐ray crystallography, NMR, and IM‐MS furnishes precise and comprehensive structural insights into the coordination complexes.
Synthesis, characterization, and a comparative study on the catalytic activity of the functionalized magnetic nanoparticles for the green oxidation of sulfides
Authors: Rayati Saeed , Mazraeh Mohammad , Shokoohi Saeedeh , Nafarieh Parinaz , Nejabat Fatemeh 
Published: July 2024

AbstractIn the following research, meso‐tetrakis(4‐carboxyphenyl)porphyrinatomanganese (III) acetate (MnTCPP(OAc)) was immobilized onto the surface of two functionalized magnetic nanoparticles (MNPs) with different linker length via amide bond (Fe3O4@SiO2‐NH2‐MnTCPP(OAc) (A1) and Fe3O4@SiO2‐NHCO‐NH2‐MnTCPP(OAc) (A2)). The prepared catalysts were characterized by standard methods such as: atomic absorption spectroscopy (AAS), Ultra violet‐visible (UV‐Vis), Fourier‐transform infrared (FT‐IR), vibrating sample magnetometry (VSM), field emission scanning electron microscopy (FE‐SEM) and transmission electron microscopy (TEM) techniques and also the effect of the length of the linker on the catalytic activity of supported magnetic nanohybrids was investigated. The prepared magnetic nano‐catalysts with different linker lengths were participated in a comparative study of the clean oxidation of sulfides with urea hydrogen peroxide (UHP) in ethanol. The higher catalytic activity, selectivity and also reusability were achieved in the presence of the catalyst with longer linker length (A2) at ambient temperature. Moreover, the turnover number (TON) of catalytic reactions using A2 as catalyst was highly enough to consider the catalyst as an efficient material to oxidize various sulfides. Both A1 and A2 catalysts simply have accomplished 5 runs of recycling without significant decrease in the catalytic activity.
Influence of nutrition during previtellogenesis on the follicular development in the Asian Tiger mosquito, Aedes albopictus (Skuse) (Diptera: Culicidae)
Authors: Yamany Abeer S. , Abdel‐Gaber Rewaida 
Published: July 2024

AbstractDengue fever poses a global public health threat, with 2.5 billion people at risk of infection each year. Because the Aedes albopictus is the primary vector of dengue, it is closely monitored and handled. The efficiency of Dengue eradication is strongly dependent on understanding a female mosquito's physiological age. This study addresses key entomological issues about the impact of previtellogenic nutrition on egg production mechanisms. Ovarian development included two distinct periods: previtellogenesis and vitellogenesis. Sugar intake during previtellogenesis influences the size of the blood meal. The major parameter influencing the vitellogenesis process is the presence of a hematophagous feeding event following sugar concentration. Upon subjecting female mosquitoes to sucrose, the ovarian follicles entered the third phase of previtellogenesis. Once females feed on blood following sucrose, ovarian development enters the vitellogenesis, and the oocyte cytoplasm reveals that the yolk granules are organized in one or two rows like a crown, increasing oocyte size. Females fed 15% sucrose before a blood meal, have the largest vitellogenic growth, and follicular size, which is seven times greater than those fed water only. Fecundity increased by 78.7% by adding 7% sucrose to the diet. Mitochondria within oocytes increase, most likely due to their transportation from the nurse cells, where the yolk is synthesized. This study describes in detail the histological alterations detected in the ovaries during the previtellogenesis as well as those associated with yolk formation, suggesting that yolk protein deposition in the oocyte is associated with blood meal, independent of sucrose feeding.Research Highlights
Adult nutrition during previtellogenesis significantly impacts various biological parameters and the physiological age of adults of Aedes albopictus.
Female mosquitoes experienced significant growth in vitellogenic development, vectorial capacity, and follicular size after consuming a diet with 15% sucrose before a blood meal.
Australians with metabolic dysfunction‐associated steatotic liver disease have a twofold increase in the incidence of cancer
Authors: Powell Elizabeth E , Roche Shruti , Sarraf Babak , Hartel Gunter , Skoien Richard , Leggett Barbara , O'Beirne James , Valery Patricia C 
Published: July 2024

AbstractBackground and AimMetabolic dysfunction‐associated steatotic liver disease (MASLD) is associated with an increased risk of extrahepatic morbidity. We compared the incidence of cancers in adults admitted to Queensland hospitals with MASLD with that for the Queensland population and examined the association between cirrhosis and type 2 diabetes and the development of extrahepatic cancers.MethodsIn this retrospective study, we identified all cancers (Queensland Cancer Registry) after the first hospitalization with MASLD during Jul‐2007 to Dec‐2019, estimated age‐standardized incidence (ASI) of cancers, and compared that with the ASI in the Queensland population (incidence rate ratios [IRR]). Among the MASLD cohort, we examined the association between diabetes and cancer risk (Cox regression). Median follow‐up was 3.8 years (54 204 person‐years).ResultsTotally 1104 new cancers were diagnosed in 1018 patients (8.9% of 9771 non‐cirrhotic and 1712 adults with cirrhosis). The ASI (all cancers) of 1668.2 per 100 000 person‐years in men (95% CI 1523.7–1827.4) and 1284.0 per 100 000 person‐years in women (95% CI 1169.6–1408.2) was 2‐fold higher than that of the Queensland population (IRR = 1.94, 95% CI 1.75–2.16 and IRR = 1.99, 95% CI 1.78–2.22, respectively). Incidence of stomach cancer, unknown primary, and pancreas was 3‐ to 5‐fold higher compared to the general population (all P < 0.001). In multivariable analysis of the MASLD cohort, older age (e.g. ≥70 years adjusted hazard ratio [adj‐HR] = 4.59, 95% CI 3.61–5.83), male gender (adj‐HR = 1.20, 95% CI 1.05–1.37), and cirrhosis (adj‐HR = 1.37, 95% CI 1.11–1.70) were independently associated with extrahepatic cancer risk, while diabetes was not.ConclusionsOur findings will help to raise awareness among clinicians about the importance of cancer vigilance in this patient group.
Low HIV drug resistance prevalence among recently diagnosed HIV‐positive men who have sex with men in a setting of high PrEP use
Authors: King Jonathan M , Giallonardo Francesca Di , Shaik Ansari , McGregor Skye , Yeung Julie Yuek Kee , Sivaruban Tharshini , Lee Frederick J , Cunningham Philip , Dwyer Dominic E , Nigro Steven J , Grulich Andrew E , Kelleher Anthony D 
Published: July 2024

AbstractIntroductionNew South Wales (NSW) has one of the world's highest uptake rates of HIV pre‐exposure prophylaxis (PrEP). This uptake has been credited with sharp declines in HIV transmission, particularly among Australian‐born gay and bisexual men. Concerns have been raised around the potential for the emergence of tenofovir (TFV) and XTC (lamivudine/emtricitabine) resistance in settings of high PrEP use. Such an emergence could also increase treatment failure and associated clinical outcomes among people living with HIV (PLHIV). Despite low levels of nucleoside reverse‐transcriptase inhibitor (NRTI) resistance relating to PrEP use in clinical settings, there are few published studies describing the prevalence of NRTI resistance among people newly diagnosed with HIV in a setting of high PrEP use.MethodsUsing HIV antiretroviral drug resistance data linked to NSW HIV notifications records of people diagnosed from 1 January 2015 to 31 December 2021 and with HIV attributed to male‐to‐male sex, we described trends in TFV and XTC resistance. Resistance was identified using the Stanford HIV Drug Resistance genotypic resistance interpretation system. To focus on transmitted drug resistance, resistance prevalence estimates were generated using sequences taken less than 3 months post‐HIV diagnosis. These estimates were stratified by timing of sequencing relative to the date of diagnosis, year of sequencing, birthplace, likely place of HIV acquisition, and stage of HIV at diagnosis.ResultsAmong 1119 diagnoses linked to HIV genomes sequenced less than 3 months following diagnosis, overall XTC resistance prevalence was 1.3%. Between 2015 and 2021, XTC resistance fluctuated between 0.5% to 2.9% and was 1.0% in 2021. No TFV resistance was found over the study period in any of the sequences analysed. Higher XTC resistance prevalence was observed among people with newly acquired HIV (evidence of HIV acquisition in the 12 months prior to diagnosis; 2.9%, p = 0.008).ConclusionsIn this Australian setting, TFV and XTC resistance prevalence in new HIV diagnoses remained low. Our findings offer further evidence for the safe scale‐up of PrEP in high‐income settings, without jeopardizing the treatment of those living with HIV.
Cost‐effectiveness analysis of community‐led HIV self‐testing among key populations in Côte d'Ivoire, Mali, and Senegal
Authors: Lu Ingrid Jiayin , Silhol Romain , d'Elbée Marc , Boily Marie‐Claude , Soni Nirali , Ky‐Zerbo Odette , Vautier Anthony , Simo Fosto Artlette , Badiane Kéba , Traoré Metogara , Terris‐Prestholt Fern , Larmarange Joseph , Maheu‐Giroux Mathieu ,   for the ATLAS Team
Published: July 2024

ABSTRACTIntroductionHIV self‐testing (HIVST) is a promising strategy to improve diagnosis coverage among key populations (KP). The ATLAS (Auto Test VIH, Libre d'Accéder à la connaissance de son Statut) programme implemented HIVST in three West African countries, distributing over 380,000 kits up between 2019 and 2021, focussing on community‐led distribution by KP to their peers and subsequent secondary distribution to their partners and clients. We aim to evaluate the cost‐effectiveness of community‐led HIVST in Côte d'Ivoire, Mali and Senegal.MethodsAn HIV transmission dynamics model was adapted and calibrated to country‐specific epidemiological data and used to predict the impact of HIVST. We considered the distribution of HIVST among two KP—female sex workers (FSW), and men who have sex with men (MSM)—and their sexual partners and clients. We compared the cost‐effectiveness of two scenarios against a counterfactual without HIVST over a 20‐year horizon (2019–2039). The ATLAS‐only scenario mimicked the 2‐year implemented ATLAS programme, whereas the ATLAS‐scale‐up scenario achieved 95% coverage of HIVST distribution among FSW and MSM by 2025 onwards. The primary outcome is the number of disability‐adjusted life‐years (DALY) averted. Scenarios were compared using incremental cost‐effectiveness ratios (ICERs). Costing was performed using a healthcare provider's perspective. Costs were discounted at 4%, converted to $USD 2022 and estimated using a cost‐function to accommodate economies of scale.ResultsThe ATLAS‐only scenario was highly cost‐effective over 20 years, even at low willingness‐to‐pay thresholds. The median ICERs were $126 ($88–$210) per DALY averted in Côte d'Ivoire, $92 ($88–$210) in Mali and 27$ ($88–$210) in Senegal. Scaling‐up the ATLAS programme would also be cost‐effective, and substantial epidemiological impacts would be achieved. The ICERs for the scale‐up scenario were $199 ($122–$338) per DALY averted in Côte d'Ivoire, $224 ($118–$415) in Mali and $61 ($18–$128) in Senegal.ConclusionsBoth the implemented and the potential scale‐up of community‐led HIVST programmes in West Africa, where KP are important to overall transmission dynamics, have the potential to be highly cost‐effective, as compared to a scenario without HIVST. These findings support the scale‐up of community‐led HIVST to reach populations that otherwise may not access conventional testing services.
Alveolar Soft Tissue Sarcoma: Correlation of MRI Features With Histological Grading and Patient Prognosis
Authors: Yuan Junhui , Xie Deshun , Fang Shaobo , Meng Fan , Shan Dongqiu , Wang Yuanyuan , Du Xinhui , Xu Chunmiao , Zhang Renzhi , Chen Xuejun 
Published: July 2024

BackgroundAlveolar Soft Part Sarcoma (ASPS) is a rare, aggressive cancer whose diagnosis and treatment depend on histological grading. However, tumor variability can lead to underestimation, affecting treatment, and patient survival.ObjectiveTo evaluate MRI features associated with Grade III ASPS and to determine the relationship between MRI features and patient prognosis.Study TypeRetrospective analysis.SubjectsSixty‐seven patients with ASPS were included with 37 males and 30 females (M/F = 1.23) follow‐up and survival analysis on 50 patients.Field Strength/SequenceA 3.0 T, T1WI‐FSE, T2WI‐FSE, DWI‐EPI, DCE‐MRI (gradient echo).AssessmentMRI features (margin, peritumoral oedema, peritumoral enhancement, necrosis, vascular flow void signal, heterogeneous signal intensity [SI] at T1WI and T2WI, ADCmean, time‐intensity curve [TIC] type, distant metastasis, and bone invasion) and histological grading were independently evaluated by three radiologists and two pathologists, with Grade III considered high‐grade.Statistical TestsThe chi‐square or Fisher's exact test was used to assess the correlation between MRI features and histological grading. Multivariable binary logistic regression identified independent factors associated with high‐grade tumors. The Kaplan–Meier method and Cox proportional hazards model were used to calculate hazard ratios for MRI features.ResultsTumor necrosis, heterogeneous SI at T2WI ≥50%, and ADCmean were associated with high‐grade ASPS. Tumor necrosis was an independent factors associated with local relapse‐free survival (odds ratio [OR], 3.88). TIC type was associated with 5‐year survival rate (OR, 2.80) and local relapse‐free survival (OR, 2.69). Heterogeneous SI at T2WI ≥50% was associated with 5‐year survival (OR, 4.00), local relapse‐free survival (OR, 5.58), and local relapse‐free survival (OR, 4.84).Data ConclusionMRI features including tumor necrosis, heterogeneity of SI at T2WI, ADCmean, and TIC type aid in assessing ASPS grading and prognosis.Evidence Level4Technical EfficacyStage 5
MicroRNA‐26a deficiency attenuates the severity of frozen shoulder in a mouse immobilization model
Authors: Sumimoto Yasuhiko , Harada Yohei , Yimiti Dilimulati , Watanabe Chikara , Miyaki Shigeru , Adachi Nobuo 
Published: July 2024

AbstractThe main pathogenesis of the frozen shoulder is thought to be the inflammation of the intra‐articular synovium and subsequent fibrosis of the shoulder joint capsule. However, the molecular pathogenesis of the frozen shoulder is still unknown. A class of noncoding RNAs, microRNAs contribute to various diseases including musculoskeletal diseases. MicroRNA‐26a (miR‐26a) has been reported to be associated with fibrosis in several organs. This study aims to reveal the role of miR‐26a on fibrosis in the shoulder capsule using a frozen shoulder model in miR‐26a deficient (miR‐26a KO) mice. MiR‐26a KO and wild‐type (WT) mice were investigated using a frozen shoulder model. The range of motion (ROM) of the shoulder, histopathological changes such as synovitis, and fibrosis‐related gene expression in the model mice were evaluated to determine the role of miR‐26a. In WT mice, both inflammatory cell infiltration and thickening of the inferior shoulder joint capsule were observed after 1 week of immobilization, and this thickening further progressed over the subsequent 6 weeks. However, the immobilized shoulder in miR‐26a KO mice consistently exhibited significantly better ROM compared with WT mice at 1 and 6 weeks, and histological changes were significantly less severe. The expression of inflammation‐ and fibrosis‐related genes was decreased in the miR‐26a KO mice compared with WT mice at 1 and 6 weeks. Together, miR‐26a deficiency attenuated the severity of frozen shoulder in the immobilization model mouse. The present study suggests that miR‐26a has the potential to be a target miRNA for therapeutic approach to frozen shoulder.
Depth perception optimization of mixed reality simulation systems based on multiple‐cue fusion
Authors: Wang Wei , Chen Tong , Liu Haiping , Zhang Jiali , Wang Qingli , Jiang Qinsheng 
Published: July 2024

AbstractMixed reality technology can be applied to simulation training, improve surgical performance, enhance 3D game experience, and so on, attracting extensive attention of researchers. The perception of the user about head‐mounted display MR using HoloLens is critical, especially for precision applications such as virtual hoisting. Designing and adding appropriate depth cues in MR scenes is an effective way to improve users' depth perception. In this study, taking virtual hoisting training system as an example, the depth perception strategy of multi‐cue fusion is proposed to improve the perception effect. Based on the mechanism of human depth perception, five kinds of depth cues are designed. The depth perception effect of adding single cue is studied by perceptual matching experiment. Based on the principle of fuzzy clustering, a multiple‐cue comprehensive depth optimization strategy on viewing distance scale is proposed. Finally, the perceptual matching results demonstrate the effectiveness of the multi‐cue fusion strategy, and the average error is reduced by 20.68% compared with the single‐cue strategy, which can significantly improve the spatial depth perception. This research can provide a reference for improving users' depth perception in interactive MR simulation systems.
Magnetic molecularly imprinted polymer based on coordination for the determination of trace banned substance furosemide in human urine
Authors: He Kunlin , Chen Qian , Chen Xueling , Zhang Chungu , Feng Shun , Shan Lianhai 
Published: July 2024

Furosemide (FUR), banned in sports events by the World Anti‐Doping Agency, is a key target in drug tests, necessitating a pretreatment material capable of selectively, rapidly, and sufficiently separating/enriching analytes from complex matrices. Herein, a metal‐mediated magnetic molecularly imprinted polymer (mMIP) was rationally designed and synthesized for the specific capture of FUR. The preparations involved the utilization of chromium (III) as the binding pivot, (3‐aminopropyl)triethoxysilane as functional monomer, and Fe3O4 as core, all assembled via free radical polymerization. Both the morphologies and adsorptive properties of the mMIP were characterized using multiple methods. The resulting Cr(III)‐mediated mMIP (ChM‐mMIP) presented excellent selectivity and specificity toward FUR. Under optimized conditions, the adsorption capacity reached 128.50 mg/g within 10 min, and the imprinting factor was 10.41. Moreover, it was also successfully applied as a dispersive solid‐phase extraction material, enabling the detection of FUR concentration as low as 20 ng/mL in human urine samples when coupled with a high‐performance liquid chromatography/photodiode array. Overall, this study offers a valuable strategy for the development of novel recognition material.
Indirect and sensitive determination of microRNAs by magnetic field‐assisted capillary sieving electrophoresis combined with catalytic hairpin assembly
Authors: Ning Jinfeng , Ye Junlan , Wang Qingqing , Wang Wei 
Published: July 2024

To determine multiple microRNAs (miRNAs) from cells simultaneously is essential for understanding biological functions. Capillary electrophoresis (CE) can simultaneously determine multiple miRNAs by separation. Nevertheless, similar lengths and low concentrations in cells make miRNAs hard to separate and detect. In this study, CE with laser‐induced fluorescence detection was combined with catalytic hairpin assembly (CHA) to determine three miRNAs, miR‐21, miR‐31, and miR‐122. The amplification products of CHA, which were DNA duplexes, were designed to have different lengths for different miRNAs. This allowed for easy separation of the duplexes of different miRNAs by CE. The indirect determination of miRNAs was then achieved by separating and detecting these duplexes. A magnetic field was first applied on the capillary sieving electrophoresis to assist in the separation of the duplexes. Under the optimal conditions, the three duplexes could be completely separated within 2.5 min with the detection limits of miRNAs in the range 1.12–4.05 × 10−15 M. MiR‐21 and miR‐31 were successfully determined from Hela cells, while miR‐122 was determined from chicken livers by this method. The recoveries ranged from 97.5% to 118%. The developed method was sensitive and reliable for miRNA determination.
Multiple analyses of main flavor components in reconstituted tobacco and transfer behavior of their key substances during heating
Authors: Shi Jianyang , Yang Yunxia , Zhang Ting , Liang Kun , Guo Linqing , Deng Ruijie , Liu Kai , Ren Yao 
Published: July 2024

Reconstituted tobacco (RT) is a product made by reprocessing tobacco waste, experiencing a growing demand for heat‐not‐burn products. The purpose of this study is to analyze the main flavor ingredients in RT aerosol, as well as the transfer behavior of key flavor substances from substrates to aerosol and the concentrations of these compounds in the substrate after heating. First, we demonstrated that the odor of four RT aerosol samples could be distinguished using an electronic nose. Through non‐targeted analysis, 93 volatile compounds were detected by gas chromatography‐mass spectrometry, and 286 non/semi‐volatile compounds were identified by ultra‐high‐performance liquid electrophoresis chromatography‐mass spectrometry in aerosol. Furthermore, we found that the formation of RT aerosol involves primarily evaporation and distillation, however, the total content delivered from unheated RT samples to aerosol remains relatively low due to compound volatility and cigarette filtration. Thermal reactions during heating indicated the pyrolysis of chlorogenic acid to generate catechol and resorcinol, while Maillard reactions involving glucose and proline produced 2,3‐dihydro‐3,5‐dihydroxy‐6‐methyl‐4h‐pyran‐4‐one. The study highlighted that heating RT at approximately 300°C could mitigate the production of harmful substances while still providing a familiar sensory experience with combusted tobacco.