bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relation between low 50‐g glucose challenge test results and small‐for‐gestational‐age infants
Authors: Shinohara Satoshi , Hirai Mitsuo , Hirata Shuji , Suzuki Kohta 
Published: July 2015

AbstractAimThere is no consensus opinion regarding a possible relation between low 50‐g glucose challenge test (GCT) results and small‐for‐gestational‐age infants (SGA). This study aimed to clarify the relation between low 50‐g GCT results and SGA, after adjusting for potential confounding factors, using a large clinical dataset.Material and MethodsOur retrospective cohort study evaluated the obstetric records of women who delivered at Kofu Municipal Hospital (Japan) between January 2011 and December 2013. The exclusion criteria were gestational diabetes mellitus, diabetes in pregnancy, and GCT results of ≥140 mg/dL. Based on these criteria, we included 1603 women who had undergone a GCT between 24 and 28 weeks of gestation, and divided the subjects into a low‐GCT group (≤90 mg/dL) and a non‐low‐GCT group (91–139 mg/dL). The χ2‐test and multivariate logistic regression analysis were used to investigate the association between low GCT results and SGA.ResultsThe mean subject age was 30.4 ± 5.4 years, with 45.1% nulliparity (n = 723) and 1538 (96.0%) term deliveries. The incidence of SGA was 10.7% (172/1603), and 17.9% (287/1603) of the women had low GCT results. Low GCT results were significantly associated with an increased risk of SGA (odds ratio, 1.66; 95% confidence interval, 1.14–2.42), after controlling for maternal age, pre‐pregnancy maternal weight, maternal weight gain during pregnancy, and pregnancy‐induced hypertension.ConclusionIt appears that there is a significant association between low GCT results and SGA. However, further studies are needed to explore the detailed mechanisms of this association.
Effectiveness of Biodegradable Magnesium Alloy Stents in Coronary Artery and Femoral Artery
Authors: Yue Yunan , Wang Lili , Yang Nuo , Huang Jinglin , Lei Licheng , Ye Huiming , Ren Lihui , Yang Shuixiang 
Published: July 2015

ObjectivesTo access the biocompatibility, effectiveness, and safety of biodegradable magnesium (Mg) alloy stent (BMAS) in the coronary artery and femoral artery.BackgroundAtherosclerosis is a lesion of cardiovascular system, including the diseases in heart and blood vessels.MethodsThe aluminum (Al) and zinc (Zn)‐based BMAS was designed by cold drawing methods. Forty healthy immunized mongrel dogs were randomly divided into 8 groups. Five dogs who have not been treated with stent were included in control group. The other dogs were implanted with an absorbable magnesium (Mg) alloy in the coronary and/or femoral artery, and their artery angiography were observed at 7 time points (1, 3, 5, 7, 14, 21, and 28 days; n = 5) follow‐up. Dogs from each cohort were sacrificed following angiography for pathology assessment. The histological response including inflammatory response, thrombosis, and intimal hyperplasia were analyzed by hematoxylin‐eosin staining. Lumen area (La), intimal hyperplasia area (IHa), and the ratio of IHa were calculated by image analysis software.ResultsThe thin‐walled BMAS were designed and produced by cold‐drawing technology. Fifty‐one devices were implanted into coronary artery of 35 dogs successfully. During the follow‐up days, the angiography of coronary artery and femoral artery had confirmed that the lumen was clear and there were no elastic recoil and thrombosis. The stents were completely disappeared at 7 days after implantation. Moderate intimal hyperplasia was found at 14 days after implantation.ConclusionThe BMAS stent proved to be of good biocompatibility, safety, and effectiveness. (J Interven Cardiol 2015;XXXX:XX–XX)
Feasibility and Efficacy of Herculink Elite (TM) Biliary Stent Implantation in Large Coronary Arteries (≥5 mm) and Venous Conduits: An Observational Study
Authors: Viswanathan Girish N. , Din Jehangir N. , Swallow Rosie A. , Levy Terry M. , Boyd Stephen , Talwar Suneel , O'Kane Peter D. 
Published: July 2015

Background and ObjectivesPercutaneous coronary intervention (PCI) in patients with lesions of large calibre coronary arteries (≥5 mm) and saphenous venous grafts (≥5 mm) can be challenging. There are no separate guidelines available to treat these vessels with PCI. Standard coronary stents of 4 mm diameter are used to treat these lesions conventionally but carry the risk of under deployment, distortion of stent architecture and future stent thrombosis even if they are subsequently expanded beyond 5 mm.Methods and ResultsBiliary stents (Herculink Elite™) provide a better alternative to standard coronary stents in these patients. These stents are of larger diameter (5–7mm) and can be safely delivered over a 6 French sheath. In our case series, we demonstrate the use of intravascular ultrasound examination to confirm that biliary stents provide improved stent strut apposition within the coronary artery associated with extremely low repeat revascularisation rates.ConclusionOur paper highlights that PCI of lesions in patients with large calibre coronary arteries can successfully be achieved using biliary stents.
Mexican American Adolescents' Emotional Support to the Family in Response to Parental Stress
Authors: Tsai Kim M. , Gonzales Nancy A. , Fuligni Andrew J. 
Published: July 2015

In this two‐wave longitudinal, daily diary study that followed up with 421 Mexican American parent–adolescent dyads (adolescents: Mage = 15 years, 50% males) after 1 year, we investigated the contingency between parental stressors and adolescents' emotional support to family members. Adolescents provided support to their parents and other family members at similar rates, but adolescents were more likely to provide support to other family members than to their parents on days when parents experienced a family stressor. This pattern was especially pronounced in families with parents who reported physical symptoms and adolescents with a strong sense of family obligation. Adolescents' provision of emotional support was associated with same‐day feelings of role fulfillment, but not to their concurrent or long‐term psychological distress.
Complications following circumcision: Presentations to the emergency department
Authors: Gold Grace , Young Simon , O'Brien Mike , Babl Franz E 
Published: July 2015

BackgroundCircumcision is the most common surgical procedure performed on boys in Australia. Patient presentations to the emergency department (ED) following circumcision are common; however, no Australian research has investigated acute care presentations.ObjectivesTo identify reasons for presentation to the ED after circumcision and determine whether the setting (community vs. hospital) in which the procedure had been performed has any bearing on the sequelae seen.MethodsRetrospective chart review of children presenting with circumcision related problems to the Royal Children's Hospital, Melbourne, Australia, between 2012 and 2014. Descriptive and χ2 analysis included sequelae of community‐ versus hospital‐performed procedures.ResultsOver a 29‐month period, we identified 167 children with a circumcision‐related ED presentation. Mean age was 3 years. A percentage of 54.5 had been performed for non‐medical, 29.9% for medical reasons and 14.4% for reasons unknown. When location was known (n = 152), 60.5% were performed in the community and 39.5% in hospital. Reasons for presentation were: bleeding (53.9%), pain (38.3%), swelling (37.1%), redness (25.7%), decreased urine output (13.8%), fever (7.2%) and pus (6%). 29.9% were diagnosed as normal healing post circumcision. Patients were admitted in 39.1% versus 15% (P = 0.001) and re‐operated in 18.5% versus 1.7% (P = 0.001) after community‐ versus hospital‐operated circumcisions.ConclusionsA range of reasons cause patients to seek help in the ED following a circumcision. Parents would have profited from better explanation of post‐circumcision appearance of the penis. ED presentations after community‐performed procedures required more re‐operations than after hospital‐performed circumcisions.
The dysregulation of endoplasmic reticulum stress response in acute‐on‐chronic liver failure patients caused by acute exacerbation of chronic hepatitis B
Authors: Ren F. , Shi H. , Zhang L. , Zhang X. , Wen T. , Xie B. , Zheng S. , Chen Y. , Li L. , Chen D. , Duan Z. 
Published: July 2015

SummaryAlthough endoplasmic reticulum (ER) stress is critical in various liver diseases, its role in acute‐on‐chronic liver failure (AoCLF) caused by acute exacerbation of chronic hepatitis B (CHB) is still elusive. This study aimed to analyse ER stress responses in the progression of HBV‐related AoCLF. Normal liver tissues (n = 10), liver tissues of CHB (n = 12) and HBV‐related patients with AoCLF (n = 19) were used. Electron microscopy of the ultrastructure of the ER was carried out on liver specimens. The gene and protein expression levels of ER stress‐related genes were measured. We further analysed the correlation between the expression levels of ER stress‐related molecules and liver injury. Electron microscopy identified typical features of the ER microstructure in AoCLF subjects. Among the three pathways of unfolded protein responses, the PKR‐like ER kinase and inositol‐requiring enzyme 1 signalling pathway were activated in CHB subjects and inactivated in AoCLF subjects, while the activating transcription factor 6 signalling pathway was sustained in the activated form during the progression of AoCLF; the expression of glucose‐regulated protein (Grp)78 and Grp94 was gradually decreased in AoCLF subjects compared to healthy individuals and CHB subjects, showing a negative correlation with serum ALT, AST and TBIL; moreover, the ER stress‐related apoptosis molecules were activated in the progression of acute exacerbation of CHB. The dysregulated ER stress response may play a complicated role in the pathogenesis of AoCLF, and a severe ER stress response may predict the occurrence of AoCLF caused by acute exacerbation of CHB.
Ghrelin receptor modulates T helper cells during intestinal inflammation
Authors: Di Giovangiulio M. , Stakenborg N. , Bosmans G. , Meroni E. , Farro G. , Gomez‐Pinilla P. J. , Depoortere I. , Boeckxstaens G. E. , Matteoli G. 
Published: July 2015

AbstractBackgroundThe orexigenic peptide ghrelin has anti‐inflammatory properties in colitis, however, the mechanism of action and the immune cells targeted remain still to be elucidated. Here, we assessed the possible effect of ghrelin on T helper (Th) cells in a T cell transfer model of chronic colitis.MethodsDisease was induced in the recombination activating gene 1 knockout mice (Rag1−/−) by adoptive transfer of naïve Th cells from ghrelin receptor knockout mice (GRLN‐R−/−) or littermate wild‐type (WT) mice. The course and severity of colitis was assessed by monitoring body weight, diarrhea score, histological analysis, gene expression, and flow cytometry analysis. The possible effects of ghrelin on Th cell proliferation, polarization, and apoptosis was examined in vitro.Key ResultsOur data showed that Rag1−/− mice injected with GRLN‐R−/− Th cells displayed increased severity of colitis compared to mice injected with WT Th cells. In addition, Rag1−/− mice injected with GRLN‐R−/− Th cells had significantly higher intestinal inflammation and increased accumulation of Th1 and Th17 cells in the colon. In vitro, ghrelin directly affected proliferation of Th cells and induced apoptosis whereas it did not influence Th cell polarization.Conclusion & InferencesOur observations suggest that ghrelin modulates Th effector cells in the gut controlling proliferation and inducing apoptosis. Our findings further support the use of ghrelin as a novel therapeutic option to treat intestinal inflammatory diseases.
A Meta‐Analysis of the Relationship between Public Service Motivation and Job Satisfaction
Authors: Homberg Fabian , McCarthy Dermot , Tabvuma Vurain 
Published: July 2015

AbstractIn recent years, much research has been conducted on the relationship between public service motivation (PSM) and various outcomes, including job satisfaction. This article presents a meta‐analysis aggregating the effects ofPSMon job satisfaction. Meta‐regression analysis is used to assess the impact of numerous study characteristics and to identify potential issues of publication bias. The findings, based on 28 separate studies, show no evidence of publication bias and support the positive relationship betweenPSMand job satisfaction. Furthermore, the results support the importance of providing individuals with the opportunity to serve the public within this relationship. Given the organizational benefits that can be derived from improved job satisfaction and the focus ofPSMresearch on its implications for job satisfaction, these findings are of interest to both academics and practitioners in the field of public administration.
Quantitative Analysis of Lysobacter Predation
Authors: Seccareccia Ivana , Kost Christian , Nett Markus 
Published: August 2015

ABSTRACT

Bacteria of the genus
Lysobacter
are considered to be facultative predators that use a feeding strategy similar to that of myxobacteria. Experimental data supporting this assumption, however, are scarce. Therefore, the predatory activities of three
Lysobacter
species were tested in the prey spot plate assay and in the lawn predation assay, which are commonly used to analyze myxobacterial predation. Surprisingly, only one of the tested
Lysobacter
species showed predatory behavior in the two assays. This result suggested that not all
Lysobacter
strains are predatory or, alternatively, that the assays were not appropriate for determining the predatory potential of this bacterial group. To differentiate between the two scenarios, predation was tested in a CFU-based bioassay. For this purpose, defined numbers of
Lysobacter
cells were mixed together with potential prey bacteria featuring phenotypic markers, such as distinctive pigmentation or antibiotic resistance. After 24 h, cocultivated cells were streaked out on agar plates and sizes of bacterial populations were individually determined by counting the respective colonies. Using the CFU-based predation assay, we observed that
Lysobacter
spp. strongly antagonized other bacteria under nutrient-deficient conditions. Simultaneously, the
Lysobacter
population was increasing, which together with the killing of the cocultured bacteria indicated predation. Variation of the predator/prey ratio revealed that all three
Lysobacter
species tested needed to outnumber their prey for efficient predation, suggesting that they exclusively practiced group predation. In summary, the CFU-based predation assay not only enabled the quantification of prey killing and consumption by
Lysobacter
spp. but also provided insights into their mode of predation.
Toolbox Approaches Using Molecular Markers and 16S rRNA Gene Amplicon Data Sets for Identification of Fecal Pollution in Surface Water
Authors: Ahmed W. , Staley C. , Sadowsky M. J. , Gyawali P. , Sidhu J. P. S. , Palmer A. , Beale D. J. , Toze S. 
Published: August 2015

ABSTRACT
In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host groups (cat, cattle, dog, horse, human, and kangaroo), and 18 water samples were collected from six sites (BR1 to BR6) along the Brisbane River in Queensland, Australia. Bacterial communities in the fecal, wastewater, and river water samples were sequenced. Water samples were also tested for the presence of bird-associated (GFD), cattle-associated (CowM3), horse-associated, and human-associated (HF183) molecular markers, to provide multiple lines of evidence regarding the possible presence of fecal pollution associated with specific hosts. Among the 18 water samples tested, 83%, 33%, 17%, and 17% were real-time PCR positive for the GFD, HF183, CowM3, and horse markers, respectively. Among the potential sources of fecal pollution in water samples from the river, DNA sequencing tended to show relatively small contributions from wastewater treatment plants (up to 13% of sequence reads). Contributions from other animal sources were rarely detected and were very small (<3% of sequence reads). Source contributions determined via sequence analysis versus detection of molecular markers showed variable agreement. A lack of relationships among fecal indicator bacteria, host-associated molecular markers, and 16S rRNA gene community analysis data was also observed. Nonetheless, we show that bacterial community and host-associated molecular marker analyses can be combined to identify potential sources of fecal pollution in an urban river. This study is a proof of concept, and based on the results, we recommend using bacterial community analysis (where possible) along with PCR detection or quantification of host-associated molecular markers to provide information on the sources of fecal pollution in waterways.
Genome Sequence of a Diverse Goose Circovirus Recovered from Greylag Goose
Authors: Stenzel Tomasz , Farkas Kata , Varsani Arvind 
Published: July 2015

ABSTRACT

A diverse goose circovirus (GoCV) genome was recovered from a wild hunted greylag goose
(Anser anser
) in Poland. The genome shares 83% pairwise identity with other GoCV genomes recovered from various geese from China, Germany, and Taiwan.
First Complete Genome Sequence of Clostridium sporogenes DSM 795
T
, a Nontoxigenic Surrogate for Clostridium botulinum, Determined Using PacBio Single-Molecule Real-Time Technology
Authors: Nakano Kazuma , Terabayashi Yasunobu , Shiroma Akino , Shimoji Makiko , Tamotsu Hinako , Ashimine Noriko , Ohki Shun , Shinzato Misuzu , Teruya Kuniko , Satou Kazuhito , Hirano Takashi 
Published: July 2015

ABSTRACT

The first complete genome sequence of
Clostridium sporogenes
DSM 795
T
, a nontoxigenic surrogate for
Clostridium botulinum
, was determined in a single contig using the PacBio single-molecule real-time technology. The genome (4,142,990 bp; G+C content, 27.98%) included 86 sets of >1,000-bp identical sequence pairs and 380 tandem repeats.
Whole-Genome Sequence of the Microcystin-Degrading Bacterium
Sphingopyxis
sp. Strain C-1
Authors: Okano Kunihiro , Shimizu Kazuya , Maseda Hideaki , Kawauchi Yukio , Utsumi Motoo , Itayama Tomoaki , Zhang Zhenya , Sugiura Norio 
Published: July 2015

ABSTRACT

This report describes the whole-genome sequence of an alkalitolerant microcystin-degrading bacterium,
Sphingopyxis
sp. strain C-1, isolated from a lake in China.
Complete Genome Sequence of Acinetobacter baumannii CIP 70.10, a Susceptible Reference Strain for Comparative Genome Analyses
Authors: Krahn Thomas , Wibberg Daniel , Maus Irena , Winkler Anika , Pühler Alfred , Poirel Laurent , Schlüter Andreas 
Published: July 2015

ABSTRACT

The complete genome sequence for the reference strain
Acinetobacter baumannii
CIP 70.10 (ATCC 15151) was established. The strain was isolated in France in 1970, is susceptible to most antimicrobial compounds, and is therefore of importance for comparative genome analyses with clinical multidrug-resistant (MDR)
A. baumannii
strains to study resistance development and acquisition in this emerging human pathogen.
First Complete Genome Sequence of a Salmonella enterica subsp.
enterica
Serovar Derby Strain Associated with Pork in France
Authors: Kérouanton Annaëlle , Hirchaud Edouard , Rose Valérie , Esnault Emilie , Naquin Delphine , Denis Martine 
Published: July 2015

ABSTRACT

In France,
Salmonella enterica
subsp.
enterica
serovar Derby is one of the most often isolated serovars in pigs. Here, we describe the draft genome sequence of a strain isolated from a pig. This strain had the most frequent pulsed-field gel electrophoresis (PFGE) and antimicrobial patterns (S, SSU, T) usually observed in pig production in France. Those patterns have been also highlighted in human isolates.
Draft Genome Sequence of
Streptomyces
sp. Strain PBH53, Isolated from an Urban Environment
Authors: Gosse Jessica T. , Hill Patrick , Dowd Scot E. , Boddy Christopher N. 
Published: July 2015

ABSTRACT

We report the draft genome sequence of
Streptomyces
sp. strain PBH53, a strain isolated from an urban transit station in Ottawa, Canada. The analysis of the genome using the bioinformatics tool antiSMASH showed the presence of many unique natural product biosynthetic pathways.
Draft Genome Sequence of Triclosan-Resistant Cystic Fibrosis Isolate Achromobacter xylosoxidans CF304
Authors: Jeukens Julie , Freschi Luca , Kukavica-Ibrulj Irena , Nguyen Dao , Levesque Roger C. 
Published: July 2015

ABSTRACT

Achromobacter xylosoxidans
is an emerging opportunistic pathogen. Here, we present the genome sequence of cystic fibrosis isolate CF304. Assembly resulted in 29 contigs adding up to 6.3 Mbp. This is the second genome sequence for a cystic fibrosis isolate, and little is known about the genetic basis of pathogenicity in this organism.
Anti-CD20 Therapy Acts via FcγRIIIA to Diminish Responsiveness of Human Natural Killer Cells
Authors: Capuano Cristina , Romanelli Maddalena , Pighi Chiara , Cimino Giuseppe , Rago Angela , Molfetta Rosa , Paolini Rossella , Santoni Angela , Galandrini Ricciarda 
Published: July 2015

AbstractNatural killer (NK) immune cells mediate antibody-dependent cellular cytotoxicity (ADCC) by aggregating FcγRIIIA/CD16, contributing significantly to the therapeutic effect of CD20 monoclonal antibodies (mAb). In this study, we show that CD16 ligation on primary human NK cells by the anti-CD20 mAb rituximab or ofatumumab stably impairs the spontaneous cytotoxic response attributable to cross-tolerance of several unrelated NK-activating receptors (including NKG2D, DNAM-1, NKp46, and 2B4). Similar effects were obtained from NK cells isolated from patients with chronic lymphocytic leukemia in an autologous setting. NK cells rendered hyporesponsive in this manner were deficient in the ability of these cross-tolerized receptors to phosphorylate effector signaling molecules critical for NK cytotoxicity, including SLP-76, PLCγ2, and Vav1. These effects were associated with long-lasting recruitment of the tyrosine phosphatase SHP-1 to the CD16 receptor complex. Notably, pharmacologic inhibition of SHP-1 with sodium stibogluconate counteracted CD20 mAb-induced NK hyporesponsiveness, unveiling an unrecognized role for CD16 as a bifunctional receptor capable of engendering long-lasting NK cell inhibitory signals. Our work defines a novel mechanism of immune exhaustion induced by CD20 mAb in human NK cells, with potentially negative implications in CD20 mAb-treated patients where NK cells are partly responsible for clinical efficacy. Cancer Res; 75(19); 4097–108. ©2015 AACR.