bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mapping Functional Connectivity Signatures of Pharmacoresistant Focal Cortical Dysplasia‐Related Epilepsy
Authors: Xie Hua , Illapani Venkata Sita Priyanka , Vezina L. Gilbert , Gholipour Taha , Oluigbo Chima , Gaillard William D. , Cohen Nathan T. 
Published: August 2024

ObjectiveTo determine common network alterations in focal cortical dysplasia pharmacoresistant epilepsy (FCD‐PRE) using functional connectivity analysis of resting‐state functional magnetic resonance imaging (rsfMRI).MethodsThis is a retrospective imaging cohort from Children's National Hospital (Washington, DC, USA) from January, 2011 to January, 2022. Patients with 3‐T MRI‐confirmed FCD‐PRE underwent rsfMRI as part of routine clinical care. Patients were included if they were age 5–22 years at the time of the scan, and had a minimum of 18 months of follow‐up. Healthy, typically‐developing controls were included from Children's National Hospital (n = 16) and matched from Human Connectome Project‐Development public dataset (n = 100).ResultsA total of 42 FCD‐PRE patients (20 M:22 F, aged 14.2 ± 4.1 years) and 116 healthy controls (56 M:60 F, aged 13.7 ± 3.3 years) with rsfMRI were included. Seed‐based functional connectivity maps were generated for each FCD, and each seed was used to generate a patient‐specific z‐scored connectivity map on 116 controls. FCD‐PRE patients had mutual altered connectivity in regions of dorsal attention, default mode, and control networks. Functional connectivity was diminished within the FCD dominant functional network, as well as in homotopic regions. Cluster specific connectivity patterns varied by pathological subtype. Higher FCD connectivity to the limbic network was associated with increased odds of Engel I outcome.InterpretationThis study demonstrates diminished functional connectivity patterns in FCD‐PRE, which may represent a neuromarker for the disease, independent of FCD location, involving the dorsal attention, default mode, and control functional networks. Higher connectivity to the limbic network is associated with a seizure‐free outcome. Future multicenter, prospective studies are needed to allow for much earlier detection of signatures of treatment‐resistant epilepsy. ANN NEUROL 2025;97:51–61
Simultaneous Inhibition of Vanadium Dissolution and Zinc Dendrites by Mineral‐Derived Solid‐State Electrolyte for High‐Performance Zinc Metal Batteries
Authors: Zhou Chuancong , Wang Zeyou , Nan Qing , Wen Huan , Xu Zhenming , Zhang Jie , Zhao Zejun , Li Jing , Xing Zhenyue , Rao Peng , Kang Zhenye , Shi Xiaodong , Tian Xinlong 
Published: August 2024

AbstractDesigning solid electrolyte is deemed as an effective approach to suppress the side reaction of zinc anode and active material dissolution of cathodes in liquid electrolytes for zinc metal batteries (ZMBs). Herein, kaolin is comprehensively investigated as raw material to prepare solid electrolyte (KL−Zn) for ZMBs. As demonstrated, KL−Zn electrolyte is an excellent electronic insulator and zinc ionic conductor, which presents wide voltage window of 2.73 V, high ionic conductivity of 5.08 mS cm−1, and high Zn2+ transference number of 0.79. For the Zn//Zn cells, superior cyclic stability lasting for 2200 h can be achieved at 0.2 mA cm−2. For the Zn//NH4V4O10 batteries, stable capacity of 245.8 mAh g−1 can be maintained at 0.2 A g−1 after 200 cycles along with high retention ratio of 81 %, manifesting KL−Zn electrolyte contributes to stabilize the crystal structure of NH4V4O10 cathode. These satisfying performances can be attributed to the enlarged interlayer spacing, zinc (de)solvation‐free mechanism and fast diffusion kinetics of KL−Zn electrolyte, availably guaranteeing uniform zinc deposition for zinc anode and reversible zinc (de)intercalation for NH4V4O10 cathode. Additionally, this work also verifies the application possibility of KL−Zn electrolyte for Zn//MnO2 batteries and Zn//I2 batteries, suggesting the universality of mineral‐based solid electrolyte.
Self‐Standing Chiral Covalent Organic Framework Thin Films with Full‐Color Tunable Guest‐Induced Circularly Polarized Luminescence
Authors: Tang Xihao , Zhang Kai , Xue Ruxiao , Zheng Yuexin , Chen Simin , Zheng Shengrun , Fan Jun , Zhang Yuwei , Ye Weiping , Zhang Weiguang , Cai Songliang , Liu Yi 
Published: August 2024

AbstractExploring self‐standing chiral covalent organic framework (COF) thin films with controllable circularly polarized luminescence (CPL) is of paramount significance but remains a challenging task. Herein, we demonstrate the first example of self‐standing chiral COF films employing a polymerization‐dispersion‐filtration strategy. Pristine, low‐quality chiral COF films were produced by interfacial polymerization and then re‐dispersed into COF colloidal solutions. Via vacuum assisted assembly, these COF colloids were densely stacked and assembled into self‐standing, pure chiral COF films (L‐/D‐CCOF‐F) that were transparent, smooth, crack‐free and highly crystalline. These films were tunable in thicknesses, areas, and roughness, along with strong diffuse reflectance circular dichroism (DRCD) and cyan CPL signals, showing an intrinsic luminescence asymmetric factor (glum) of ~4.3×10−3. Furthermore, these COF films served as host adsorbents to load various achiral organic dye guests through adsorption. The effective chiral transfer and energy transfer between CCOF‐F and achiral fluorescent dyes endowed the dyes with strong chirality and tunable DRCD, resulting in intense, full‐color‐tunable solid‐state CPL. Notably, the ordered arrangement of dye guest molecules within the preferentially oriented chiral pores of CCOF‐F contributed to an amplified |glum| factor of up to 7.2×10−2, which is state‐of‐the‐art for COF‐based CPL materials. This work provides new insights into the design and fabrication of self‐standing chiral COF films, demonstrating their great potential for chiroptical applications.
Unveiling the Aggregation of M−N−C Single Atoms into Highly Efficient MOOH Nanoclusters during Alkaline Water Oxidation
Authors: Lu Shanshan , Zhang Zhipu , Cheng Chuanqi , Zhang Bin , Shi Yanmei 
Published: August 2024

AbstractM−N−C‐type single‐atom catalysts (SACs) are highly efficient for the electrocatalytic oxygen evolution reaction (OER). And the isolated metal atoms are usually considered real active sites. However, the oxidative structural evolution of coordinated N during the OER will probably damage the structure of M−N−C, hence resulting in a completely different reaction mechanism. Here, we reveal the aggregation of M−N−C materials during the alkaline OER. Taking Ni−N−C as an example, multiple characterizations show that the coordinated N on the surface of Ni−N−C is almost completely dissolved in the form of NO3−, accompanied by the generation of abundant O functional groups on the surface of the carbon support. Accordingly, the Ni−N bonds are broken. Through a dissolution‐redeposition mechanism and further oxidation, the isolated Ni atoms are finally converted to NiOOH nanoclusters supported by carbon as the real active sites for the enhanced OER. Fe−N−C and Co−N−C also have similar aggregation mechanism. Our findings provide unique insight into the structural evolution and activity origin of M−N−C‐based catalysts under electrooxidative conditions.
Skelettumlagerungen in der enzymkatalysierten Biosynthese korallenartiger Diterpene aus Chitinophaga pinensis
Authors: Tabekoueng Georges B. , Li Heng , Goldfuss Bernd , Schnakenburg Gregor , Dickschat Jeroen S. 
Published: August 2024

AbstractZwei Diterpensynthasen aus dem Bakterium Chitinophaga pinensis wurden charakterisiert. Das erste Enzym produzierte hauptsächlich das umgelagerte Diterpen Palmatol, eine aus Oktokorallen bekannte Verbindung, während das zweite Enzym das neue korallenartige Eunicellan Chitinol herstellte. Die Mechanismen beider Enzyme wurden durch Isotopenmarkierungsexperimente, DFT‐Berechnungen und mit einem Substratanalogon, das eine gesättigte Doppelbindung enthält, eingehend untersucht. Letzteres führte zur Bildung von Abbruchprodukten führte, die zusätzliche Einblicke in die Natur der Intermediate der Cyclisierungskaskade lieferten. Die Bildung korallenartiger Diterpene wirft interessante Fragen zu den Funktionen dieser Verbindungen in so unterschiedlichen Organismen wie Bakterien und Korallen auf.
Skeletal Rearrangements in the Enzyme‐Catalysed Biosynthesis of Coral‐Type Diterpenes from Chitinophaga pinensis
Authors: Tabekoueng Georges B. , Li Heng , Goldfuss Bernd , Schnakenburg Gregor , Dickschat Jeroen S. 
Published: August 2024

AbstractTwo diterpene synthases from the bacterium Chitinophaga pinensis were characterised. The first enzyme mainly produced the rearranged diterpene palmatol, a compound known from octocorals, while the second enzyme made the new coral‐type eunicellane chitinol. The mechanisms of both enzymes were deeply studied through isotopic labelling experiments, DFT calculations, and with a substrate analog containing a saturated double bond, resulting in the formation of derailment products that gave additional insights into the nature of the cyclisation cascade intermediates. The formation of coral‐type diterpenes poses interesting questions on the functions of these compounds in organisms as different as bacteria and corals.
Synergy of Rh Nanoparticles With Fe3O4 for Efficient Selective Catalytic Transfer Hydrogenation of Nitrostyrenes Using Stoichiometric Hydrazine Hydrate
Authors: Hu Ze‐Nan , Guo Fangya , Zhang Xiuxiu , Yu Jie , Yin Yaru , Zhang Biying , Chen Hua‐Jun , Chang Meijia , Wang Yiming , Miao Yalei , Zhang Lan , Tian Wenjie , Sun Hong‐bin 
Published: August 2024

ABSTRACTHydrazine hydrate as a hydrogen source has a good effect on nitro reduction, but overuse has environmental toxicity and is prone to over‐hydrogenation, resulting in low selectivity. Herein, we report a magnetically separable Rh/Fe3O4 (1.26 wt%) catalyst containing an optimal Rh content that can completely convert 3‐nitrostyrene to 3‐vinylaniline (> 99% selectivity) using a stoichiometric molar ratio of hydrazine hydrate (‐NO2:N2H4·H2O = 1:1.5). This is due to the synergy between Rh and Fe3O4 and the natural selectivity of N2H4·H2O for nitro. The synergy can reduce the activation energy of the reaction (36.6 kJ/mol), increasing the reduction rate of nitro group and avoiding the combination of active hydrogen species (H*) to H2 as well as the hydrogenation of N2H4 to NH3. Moreover, the active H* produced by N2H4·H2O is different from that produced by H2, and excess dose of N2H4 will inevitably yield H2 thus decline the selectivity.
Bioinspired Polyoxomolybdate Nanoclusters Peroxidase‐Mimicking Nanozyme for Dual‐Mode Detection of Methotrexate in Real Samples
Authors: Azarian Sina , Dehghan Gholamreza , Amini Mojtaba 
Published: August 2024

ABSTRACTMethotrexate (MTX) is a chemotherapy agent and immune system suppressant that is widely used to treat a variety of malignant and nonmalignant diseases. Due to some drawbacks of the existing methods for the determination of MTX in aquatic and real samples, we prepared a new, simple, and selective dual‐mode fluorometric and electrochemical approach based on peroxidase‐mimic [Mo36] polyoxomolybdate nanoclusters (POMo) for sensing MTX. A turn‐on–off fluorometric approach for the detection of MTX was developed, which was based on the inhibition of the emission intensity of the terephthalic acid (TA)‐H2O2 system upon the addition of MTX to the reaction solution. The electrocatalytic performance of the obtained POMo was studied after making a carbon paste electrode (CPE) utilizing cyclic voltammetry and amperometry analysis. Under the optimum condition, good linearity was observed between the quenched emission intensity of the system and MTX dosage in the range of 0.5–250 μM with a limit of detection (LOD) of 0.91 μM. The electrochemical method illustrated a much better response with an extensive linear range of 0.01–475 μM and a lower LOD (1.61 nM). Analytical recoveries and RSD% values were, respectively, in the range of 87.72%–110% and 0.33%–1.32% for the fluorometric system and 96%–106% and 0.77%–1.02% for the electrochemical platform. Finally, the selectivity of the sensor was evaluated against various common interfering species, and the results indicated satisfactory selectivity of the POMo dual‐mode system towards MTX.
Impedance‐based monitoring of titration and neutralization assays with VSV‐G and SARS‐CoV‐2‐spike pseudoviruses
Authors: Mildner Anne‐Kathrin , Einhauser Sebastian , Michaelis Stefanie , Bieberstein Klara Rogalla v. , Wagner Ralf , Wegener Joachim 
Published: August 2024

AbstractSince cell‐based virus neutralization assays are still the gold standard to assess a patient's immune protection against a given virus, they are of utmost importance for serodiagnosis, convalescent plasma therapy, and vaccine development. Monitoring the emergence and characteristics of neutralizing antibodies in an outbreak situation, confirming neutralizing antibodies as correlates of protection from infection and testing vaccine‐induced potency of neutralizing antibody responses, quests for automated, fast, and parallel neutralization assays. We developed an impedance‐based sensor platform (electric cell‐substrate impedance sensing, ECIS) providing time‐resolved monitoring of the host cell response to viral pseudotypes. For validation, the impedance assay was compared with state‐of‐the‐art quantification of virus‐induced reporter protein expression as an independent indicator of virus infection and neutralization. Vesicular stomatitis virus (VSV) derived pseudoviruses encoding the green fluorescent protein (GFP) as reporter and the autologous G protein (VSV‐G) for the initial binding to the host cell membrane were used for monitoring of HEK293T cell infection and neutralization with both, impedance and optical readout. Virus‐induced cytopathic effects (CPE) were detectable for low pseudotype concentrations (multiplicity of infection 1) in time‐resolved impedance profiles as soon as 5–10 h after infection in a concentration‐dependent manner. Neutralization efficacy of α‐VSV‐G antibodies was determined from impedance time courses and IC50 values compared favorably with fluorescence measurements of virus‐borne GFP expression. Sera of convalescent COVID‐19 patients were tested successfully for SARS‐CoV‐2 neutralizing antibodies by incubating VSV, pseudotyped with the SARS‐CoV‐2 spike protein, with different sera before host cell exposure and impedance recordings. In summary: (i) ECIS monitoring was successfully applied to detect virus‐mediated cell infection and neutralization; (ii) Impedance‐based monitoring allows reducing the assay time to 5–10 h; and (iii) the platform is easily adapted to other virus‐based diseases and scalable to high‐throughput.
Ubiquitin‐mediated endosomal stress: A novel organelle stress of early endosomes that initiates cellular signaling pathways
Authors: Endo Akinori , Komada Masayuki , Yoshida Yukiko 
Published: August 2024

AbstractCells utilize diverse organelles to maintain homeostasis and to respond to extracellular stimuli. Recently, multifaceted aspects of organelle stress caused by various factors have been emerging. The endosome is an essential organelle, functioning as the central hub for membrane trafficking in cooperation with the ubiquitin system. However, knowledge regarding endosomal stress, which refers to organelle stress of the endosome, is currently limited. We recently revealed ubiquitin‐mediated endosomal stress of early endosomes (EEs) and its responsive signaling pathways. These findings shed light on the relevance of ubiquitin‐mediated endosomal stress to physiological and pathological processes. Here, we present a hypothesis that ubiquitin‐mediated endosomal stress may have significant roles in biological contexts and that ubiquitin‐specific protease 8 is a key regulator of ubiquitin clearance from EEs.
Investigating aqueous micellar systems enhancement tools for a sensitive spectrofluorimetric determination of anti‐TB: Rifampicin
Authors: El‐Aziz Heba Abd , Samir Elama Heba 
Published: August 2024

AbstractRifampicin is a frontline antibiotic in the management of tuberculosis. Since no spectrofluorimetric methods are reported for this drug, this approach was challenged to craft a sensitive, reliable, valid, fast, and green methodology. In recent years, fluorescence spectroscopy has received a lot of interest. Its benefits include ecological greenness and analytical performance. The pharmaceutical industries greatly like this approach because of its low energy and decreased solvent usage, which make it both economical and environmentally friendly. This methodology was based on utilizing the enhanced native fluorescence of the rifampicin at 341 nm after excitation at 241 nm in a beta‐cyclodextrin micellar system. Modern developments in analytical chemistry have been applied to reduce risks to the workplace and environment by using distilled water as a dilution solvent for method application and optimization. The method was found excellent green with 97 eco‐scale and 0.86 AGREE scores besides an 89.6 overall whiteness score. The range of linearity for rifampicin raw material was 0.2–1.5 μg·mL−1, and the average recoveries for raw material and spiked plasma were 100.15% and 99.64%, respectively. The suggested technique worked well for the commercial oral syrup of Rimactane® and did not conflict with any common additives.
One‐pot derivatization spectrofluorimetric method for detection of aspartame in sweetener commercial tablets and soft drinks: Greenness assessments of the methodology
Authors: AlSalem Huda Salem , Alharbi Sara Naif , Binkadem Mona Saad , Katamesh Noha S. , Abdel‐Lateef Mohamed A. 
Published: August 2024

AbstractAspartame is an artificial sweetener used in drinks and many foods. International Agency for Research on Cancer classified aspartame as possibly carcinogenic to humans (IARC Group 2B). In this study, a sensitive and selective spectrofluorimetric method was developed to detect aspartame. The method is based on switching on the fluorescence activity of aspartame upon its condensation with O‐phthalaldehyde (Roth's reaction) in the presence of 2‐mercaptoethanol. The reaction product was detected fluorometrically at λem of 438 nm after λex of 340 nm. All reaction conditions required to yield the optimal fluorescence intensity were observed and investigated. Furthermore, the approach was validated according to ICH guidelines. Upon plotting the concentrations of aspartame against their associated fluorescence intensity values, the relationship between the two variables was linear within the range of 0.5–3.0 μg/mL. Furthermore, the method was employed to analyze the quantity of aspartame in commercial packages and soft drinks with an acceptable level of recovery. In addition, the Green Solvents Selecting Tool, Complementary Green Analytical Procedure Index, and the Analytical Greenness Metric tool were used to evaluate the sustainability and the greenness of the developed methodology.
Integrating untargeted and targeted LC–MS‐based metabolomics to identify the serum metabolite biomarkers for tuberculosis
Authors: Sa Yuping , Ding Shuqin , Zhang Yue , Wang Weibiao , Wilson Gidion , Ma Feng , Zhang Weiman , Ma Xueqin 
Published: August 2024

AbstractGiven the limitations of untargeted metabolomics in precise metabolite quantification, our current research employed a novel approach by integrating untargeted and targeted metabolomics utilizing ultra‐high‐performance liquid chromatography quadrupole time‐of‐flight tandem mass spectrometry (UHPLC‐QTOF‐MS/MS) to analyze the metabolic profile and potential biomarkers for tuberculosis (TB). A cohort of 36 TB patients and 36 healthy controls (HC) was enlisted to obtain serum samples. Multivariate pattern recognition and univariate statistical analysis were employed to screen and elucidate the differential metabolites, whereas dot plots and receiver operating characteristic (ROC) curves were established for the identification of potential biomarkers of TB. The results indicated a distinct differentiation between the two groups, identifying 99 metabolites associated with five primary metabolic pathways in relation to TB. Of these, 19 metabolites exhibited high levels of sensitivity and specificity, as evidenced by the area under curve values approaching 1. Following targeted quantitative analysis, three potential metabolites, namely, L‐asparagine, L‐glutamic acid, and arachidonic acid, were demonstrated excellent discriminatory ability as evidenced by the results of the ROC curve, dot plots, and random forest model. Particularly noteworthy was the enhanced diagnostic efficacy of the combination of these three metabolites compared to singular biomarkers, suggesting their potential utility as serum biomarkers for TB diagnosis.
The utility of behavioral activation therapy in addressing emotional problems of two depressed students with borderline intellectual disability: A case study
Authors: Alilou Majid Mahmoud , Maleki Saba 
Published: August 2024

AbstractPurposeIntellectual disability is one of the neurodevelopmental disorders. Studies indicated that depression and anxiety are the most prevalent emotional problems among the people with intellectual disability. The aim of this study was to investigate the utility of behavioral activation therapy in addressing emotional problems of two depressed students with borderline intellectual disability.MethodThis study is a single‐subject design with multiple baseline and one month follow‐up. Two students with borderline intelligence underwent behavioral activation therapy for 12 sessions. Beck Depression Inventory‐Second Edition, Beck Anxiety Inventory, Oxford Happiness Inventory and Rosenberg Self‐Esteem Scale were used. Data were analyzed using visual inspection of graphed data, changes in trends, improvement percentage and effect size.FindingsThe findings of this study support the utility of behavioral activation therapy in addressing the emotional problems of two depressed students with borderline intellectual disability.ConclusionBehavioral activation therapy has had favorable outcomes in reducing depression and anxiety in depressed students with borderline intellectual disabilities.
Analysis of Industry 4.0 technological enablers for sustainable supply chain transparency in a fuzzy environment
Authors: Rajput Shubhangini 
Published: August 2024

AbstractThis study aims to identify various Industry 4.0 technological enablers and analyze their relationships in achieving transparency in sustainable supply chains. Initially, 17 Industry 4.0 technological enablers were selected based on an extensive literature review of sustainable supply chains and evaluated through Systems and Information Processing theories. Using the fuzzy Delphi method, these enablers were then narrowed down to 14 relevant ones. The reliability of these enablers was assessed by measuring their internal consistency using Cronbach's alpha (α) and McDonald's omega (ω). The fuzzy Decision‐Making Trial and Evaluation Laboratory (DEMATEL) method was then used to demonstrate that blockchain, cybersecurity, big data, and artificial intelligence (AI) are the most prominent and influential enablers for enhancing transparency in a sustainable supply chain. Additionally, the cause–effect relationship of other key enablers provides evidence for a feasibility study that links sustainability with transparency across the supply chain. These identified Industry 4.0 technological enablers can assist external stakeholders and policymakers in improving transparency and advancing sustainable supply chain practices.