bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-brain dynamics of articulatory, acoustic and semantic speech representations
Authors: Verwoert Maxime , Amigó-Vega Joaquín , Gao Yingming , Ottenhoff Maarten C. , Kubben Pieter L. , Herff Christian 
Published: August 2024

ABSTRACTSpeech production is a complex process that traverses several representations, from the meaning of spoken words (semantic), through the movement of articulatory muscles (articulatory) and, finally, to the produced audio waveform (acoustic). In our study, we aimed to identify how these different representations of speech are spatially and temporally distributed throughout the depth of the brain. By considering multiple representations from the same exact data, we can limit potential con-founders to better understand the different aspects of speech production and acquire crucial complementary information for speech brain-computer interfaces (BCIs). Intracranial speech production data was collected of 15 participants, recorded from 1647 electrode contacts, while they overtly spoke 100 unique words. The electrodes were distributed across the entire brain, including sulci and subcortical areas. We found a bilateral spatial distribution for all three representations, although there was a stronger tuning in the left hemisphere with a more widespread and temporally dynamic distribution than in the right hemisphere. The articulatory and acoustic representations share a similar spatial distribution surrounding the Sylvian fissure, while the semantic representation appears to be widely distributed across the brain in a mostly distinct network. These results highlight the distributed nature of the speech production process and the potential of non-motor representations for speech BCIs.
Coupling of secondary metabolite production inBacillus subtilis
Authors: Dinesen Caja , Vertot Manca , Jarmusch Scott A. , Lozano-Andrade Carlos N. , Andersen Aaron J.C. , Kovács Ákos T. 
Published: August 2024

AbstractAlthough not essential for their growth, the production of secondary metabolites increases the fitness of the producing microorganisms in their natural habitat by enhancing establishment, competition and nutrient acquisition. The Gram-positive soil-dwelling bacterium,Bacillus subtilisproduces a variety of secondary metabolites. Here, we investigated the regulatory relationship between the non-ribosomal peptide surfactin and the sactipeptide bacteriocin subtilosin A. We discovered thatB. subtilismutants lacking surfactin production exhibited higher production of subtilosin A compared to their parental wild-type strain. Additionally, spatial visualization ofB. subtilisproduction of metabolites demonstrated that surfactin secreted by a wild-type colony could suppress subtilosin A production in an adjacent mutant colony lacking surfactin production. Reporter assays were performed using mutants in specific transcriptional regulators that confirmed the role of ResD as an activator of the subtilosin A encoding BGC, while removal or Rok and AbrB repressors increased expression of the BGC that was further enhanced by additional deletion of surfactin, suggesting that a so far unidentified regulator might mediate the influence of surfactin on production of subtilosin A. Our study reveals a regulatory influence of one secondary metabolite on another, highlighting that the function of secondary metabolites could be more complex than its influence on other organisms and interactions among secondary metabolites could also contribute to their ecological significance.ImportanceSecondary metabolites play an important role in the life of microorganisms facilitating their fitness in the environment, including competing against other microorganisms, interacting with their host or environment, and allowing expansion in their environment. However, secondary metabolites also function as cue molecules influencing gene expression between and within species. Here, we describe that the non-ribosomally synthesized peptide surfactin repress the production of ribosomally synthesized and post translationally modified peptide, subtilosin A inBacillus subtilis, revealing an ecological interaction between two secondary metabolites that could potentially influence the biocontrol efficiency ofB. subtilisstrain that depends on the production of these secondary metabolites against plant pathogen microorganisms.
Morpho-physiological and transcriptomic responses of field pennycress to waterlogging
Authors: Combs-Giroir Rachel , Shah Manesh , Chhetri Hari , Morgan Mallory , Prates Erica , Townsend Alice , Phippen Mary E. , Phippen Winthrop B. , Jacobson Daniel A. , Gschwend Andrea R. 
Published: August 2024

AbstractField pennycress (Thlaspi arvense) is a new biofuel winter annual crop with extreme cold hardiness and a short life cycle, enabling off-season integration into corn and soybean rotations across the Midwest. Pennycress fields are susceptible to winter snow melt and spring rainfall, leading to waterlogged soils. The objective of this research was to determine if waterlogging during the reproductive stage had a significant effect on gene expression, morphology, physiology, recovery, and yield of two pennycress lines (SP32-10 and MN106). In a controlled environment, total pod number, shoot/root dry weight, and total seed count/weight were significantly reduced in SP32-10 in response to waterlogging, whereas primary branch number, shoot dry weight, and single seed weight were significantly reduced in MN106. This indicated waterlogging had a greater negative impact on seed yield in SP32-10 than MN106. The number of differentially expressed genes (DEGs) between waterlogged and control roots were doubled in MN106 (3,424) compared to SP32-10 (1,767). Functional enrichment analysis of upregulated DEGs revealed Gene Ontology (GO) terms associated with hypoxia and decreased oxygen, with genes in these categories involved in alcoholic fermentation and glycolysis. Interestingly, MN106 waterlogged roots exhibited significant stronger upregulation of these genes than SP32-10. Additionally, downregulated DEGs revealed GO terms associated with cell wall biogenesis and secondary metabolite biogenesis, indicating suppressed growth and energy conservation. Together, these results reveal the reconfiguration of cellular and metabolic processes in response to the severe energy crisis invoked by waterlogging in pennycress.
Implementation and calibration of spectroscopic single-molecule localization microscopy
Authors: Brenner Benjamin , Yeo Wei-Hong , Lee Youngseop , Kweon Junghun , Sun Cheng , Zhang Hao F. 
Published: August 2024

AbstractSpectroscopic single-molecule localization microscopy (sSMLM) allows multi-color super-resolution images with high spectral sensitivity. In this protocol, we provide essential information for researchers to implement sSMLM in a laboratory setting. We describe how to assemble and align the illumination and detection paths of a 3D dual-wedge prism (DWP)-based sSMLM instrument. We provide detailed step-by-step instructions for performing spectral and axial calibration using fluorescent beads and a nanohole array, respectively. We also discuss using sSMLM to image fluorescently labeled cells and report a new MATLAB package, RainbowSTORM v2, to reconstruct super-resolution 3D images. Further, we present representative images as typical anticipated results for users to validate.
What decision-making process do mHealth users go through when faced with privacy disclosure behaviors? A dual trade-off perspective
Authors: Xin Hao , Liu FengTao , Wei ZiXiang 
Published: August 2024

PurposeThis paper proposes that the trade-off between medical benefits and privacy concerns among mHealth users extends to their disclosure intentions, manifested as individuals simultaneously holding intentions to tend to disclose in the near future and to reduce disclosure in the distant future. Consequently, this paper aims to explore the privacy decision-making process of mHealth users from the perspective of a dual trade-off.Design/methodology/approachThis paper constructs the model using the privacy calculus theory and the antecedent-privacy concern-outcome framework. It employs the construal level theory to evaluate the impact of privacy calculus on two types of disclosure intentions. The study empirically tests the model using a data sample of 386 mHealth users.FindingsThe results indicate that perceived benefits positively affect both near-future and distant-future disclosure intentions. In contrast, perceived risks just negatively affect distant-future disclosure intention. Additionally, perceived benefits, near-future and distant-future disclosure intentions positively affect disclosure behavior. The findings also reveal that privacy management perception positively affects perceived benefits. Personalized services and privacy invasion experience positively affect perceived benefits and risks, while trust negatively affects perceived risks.Originality/valueThis paper considers the trade-off in the privacy calculus phase as the first trade-off. On this basis, this trade-off will extend to the disclosure intention. The individuals’ two times of trade-offs between privacy concerns and medical benefits constitute the dual trade-off perspective. This paper first uses this perspective to explore the privacy decision-making process of mHealth users. This paper employs the construal level theory to effectively evaluate the impact of privacy calculus on both disclosure intentions in mHealth, extending the theory’s applicability. Moreover, we introduce antecedents of privacy calculus from the perspectives of platform, society, and individuals, enhancing the study’s realism. The research findings provide a basis for mHealth platforms to better cater to users’ privacy needs.
When the well is full, it will run over: the double-edged sword effect of corporate lobbying activities on firm performance
Authors: Xiao Shufeng , Jiménez Alfredo , Jung Sukyoon , Park Byung Il , Choi Seong Jin 
Published: August 2024

PurposeHow much variance in firm performance can be attributed to firms’ corporate political activities (CPA)? Under what conditions does CPA contribute to firm performance? To theorize and empirically tackle these questions, we build on the resource-based view (RBV) to theorize how CPA might improve or hinder firm performance, and specifically examine the direct relationship between firms’ investments in lobbying activities and their performance. We also expect firm growth rate to moderate the relationship between lobbying and performance.Design/methodology/approachWe empirically test our hypotheses using large-scale longitudinal panel data from publicly traded US firms from 2008 to 2018.FindingsOur analyses support our predictions of the double-edged sword effect of lobbying on firm performance. Moreover, our results show that this effect is steeper for firms with higher growth rates.Originality/valueOur study contributes meaningful insights to strategy scholarship on the influence of nonmarket strategies, highlighting the relevance of firm-specific conditions in shaping the performance outcomes of such strategies. In particular, we make a contribution by identifying a nonlinear relationship between lobbying and firm performance, which is amplified in fast-growing firms compared to stagnant ones.
ConjunctiveNet: an improved deep learning-based conjunctive-eyes segmentation and severity detection model
Authors: Pahwa Seema , Kaur Amandeep , Dhiman Poonam , Damaševičius Robertas 
Published: August 2024

PurposeThe study aims to enhance the detection and classification of conjunctival eye diseases' severity through the development of ConjunctiveNet, an innovative deep learning framework. This model incorporates advanced preprocessing techniques and utilizes a modified Otsu’s method for improved image segmentation, aiming to improve diagnostic accuracy and efficiency in healthcare settings.Design/methodology/approachConjunctiveNet employs a convolutional neural network (CNN) enhanced through transfer learning. The methodology integrates rescaling, normalization, Gaussian blur filtering and contrast-limited adaptive histogram equalization (CLAHE) for preprocessing. The segmentation employs a novel modified Otsu’s method. The framework’s effectiveness is compared against five pretrained CNN architectures including AlexNet, ResNet-50, ResNet-152, VGG-19 and DenseNet-201.FindingsThe study finds that ConjunctiveNet significantly outperforms existing models in accuracy for detecting various severity stages of conjunctival eye conditions. The model demonstrated superior performance in classifying four distinct severity stages – initial, moderate, high, severe and a healthy stage – offering a reliable tool for enhancing screening and diagnosis processes in ophthalmology.Originality/valueConjunctiveNet represents a significant advancement in the automated diagnosis of eye diseases, particularly conjunctivitis. Its originality lies in the integration of modified Otsu’s method for segmentation and its comprehensive preprocessing approach, which collectively enhance its diagnostic capabilities. This framework offers substantial value to the field by improving the accuracy and efficiency of conjunctival disease severity classification, thus aiding in better healthcare delivery.
Heritage conceptions, perceptions and learning context: research on primary and secondary schools in Andorra
Authors: Ballesté Marc , Fernández Ares , Yáñez de Aldecoa Cristina , Solé-Llussà Anna 
Published: August 2024

PurposeHeritage education is currently a consolidated discipline, which advocate for a holistic vision of heritage. In this sense, this research aims to study the heritage conceptions, perceptions and learning context amongst primary and secondary students, comprehensively and fully.Design/methodology/approachThis research focuses in Andorra, a country in the Pyrenees, where there are three different education systems. This allowed the study to obtain a sample of 1,235 primary and secondary students, throughout a structured questionnaire that was previously designed and validated ad hoc.FindingsIn general terms, the results show that around half of the students have a holistic view of heritage; however, natural and historical elements are highlighted as the ones they learn from the most, especially through visits in situ. Moreover, the students’ perceptions of heritage show that over a 90% of students believe it is important to safeguard because it is connected to nature and culture preservation.Originality/valueThis research is included in a greater scope project that also considers other agents in the education community that belong to formal and non-formal spheres. In addition, it is the first investigation where the field of heritage education is studied globally in an entire country, considering primary and secondary education students.
The effect of tailored reciprocity on information provision in an investigative interview
Authors: Weiher Lynn , Winters Christina , Taylor Paul , Luther Kirk , Watson Steven James 
Published: August 2024

Purpose
In their study of reciprocity in investigative interviews, Matsumoto and Hwang (2018) found that offering interviewees water prior to the interview enhanced observer-rated rapport and positively affected information provision. This paper aims to examine whether tailoring the item towards an interviewee’s needs would further enhance information provision. This paper hypothesised that interviewees given a relevant item prior to the interview would disclose more information than interviewees given an irrelevant item or no item.

Design/methodology/approach
Participants (n = 85) ate pretzels to induce thirst, engaged in a cheating task with a confederate and were interviewed about their actions after receiving either no item, an irrelevant item to their induced thirst (pen and paper) or a relevant item (water).

Findings
This paper found that receiving a relevant item had a significant impact on information provision, with participants who received water providing the most details, and significantly more than participants that received no item.

Research limitations/implications
The findings have implications for obtaining information during investigative interviews and demonstrate a need for research on the nuances of social reciprocity in investigative interviewing.

Practical implications
The findings have implications for obtaining information during investigative interviews and demonstrate a need for research on the nuances of social reciprocity in investigative interviewing.

Originality/value
To the best of the authors’ knowledge, this study is the first to experimentally test the effect of different item types upon information provision in investigative interviews.
Finite element analysis of Cu-water nanofluid flow and heat transfer in a dynamically bulging enclosure
Authors: Chuhan Imran Shabir , Li Jing , Ahmed Muhammad Shafiq , Jamil Muhammad Ashfaq , Ejaz Ahsan 
Published: August 2024

PurposeThe main purpose of this study is to analyze the heat transfer phenomena in a dynamically bulging enclosure filled with Cu-water nanofluid. This study examines the convective heat transfer process induced by a bulging area considered a heat source, with the enclosure's side walls having a low temperature and top and bottom walls being treated as adiabatic. Various factors, such as the Rayleigh number (Ra), nanoparticle volume fraction, Darcy effects, Hartmann number (Ha) and effects of magnetic inclination, are analyzed for their impact on the flow behavior and temperature distribution.Design/methodology/approachThe finite element method (FEM) is employed for simulating variations in flow and temperature after validating the results. Solving the non-linear partial differential equations while incorporating the modified Darcy number (10−3 ≤ Da ≤ 10−1), Ra (103 ≤ Ra ≤ 105) and Ha (0 ≤ Ha ≤ 100) as the dimensionless operational parameters.FindingsThis study demonstrates that in enclosures with dynamically positioned bulges filled with Cu-water nanofluid, heat transfer is significantly influenced by the bulge location and nanoparticle volume fraction, which alter flow and heat patterns. The varying impact of magnetic fields on heat transfer depends on the Rayleigh and Has.Practical implicationsThe geometry configurations employed in this research have broad applications in various engineering disciplines, including heat exchangers, energy storage, biomedical systems and food processing.Originality/valueThis research provides insights into how different shapes of the heated bulging area impact the hydromagnetic convection of Cu-water nanofluid flow in a dynamically bulging-shaped porous system, encompassing curved surfaces and various multi-physical conditions.
Debt advice in Europe: a search of the good practices to fight over-indebtedness
Authors: Tanda Alessandra , Vandone Daniela 
Published: August 2024

Purpose
This paper aims to provide an overview of the current state of debt advisory services and good practices in Europe.

Design/methodology/approach
The authors examine how debt advisory services are organised in different European countries and how they can be used to address the phenomenon of over-indebtedness.

Findings
Debt advisory services seem to be varied and fragmented. There are few good practices that stand out, whereas in some countries there are no services available at all.

Originality/value
This study provides an updated and comprehensive review of good practices and suggests some measures for evaluating the effectiveness of debt advisory services.
Incorporating density‐dependent regulation into impact assessments for seabirds
Authors: Merrall Eve , Green Jonathan A. , Robinson Leonie A. , Butler Adam , Wood Matt J. , Newell Mark A. , Black Julie , Daunt Francis , Horswill Catharine 
Published: August 2024

Abstract

Many industries are required to perform population viability analysis (PVA) during the consenting process for new developments to establish potential impacts on protected populations. However, these assessments rarely account for density‐dependent regulation of demographic rates. Excluding density‐dependent regulation from PVA‐based impact assessments is often assumed to provide a maximum estimate of impact and therefore offer a precautionary approach to assessment. However, there is also concern that this practice may unnecessarily impede the development of important industries, such as offshore renewable energy.

In this study, we assess density‐dependent regulation of breeding success in 31 populations of seabird. We then quantify the strength and form of this regulation using eight different formulations. Finally, we use PVA to examine how each formulation influences the recreation of observed dynamics (i.e. model validation), as well as the predicted absolute and relative population response to an extrinsic threat (i.e. model projection).

We found evidence of both negative (n = 3) and positive (n = 5) regulation of seabird breeding success. In populations exhibiting negative regulation, excluding density‐dependent regulation from PVA‐based impact assessment allowed uncontrolled population growth, such that model outcomes became biologically implausible. By contrast, in populations exhibiting positive regulation, excluding density‐dependent regulation provided an appropriate reconstruction of observed dynamics, but population decline was underestimated in some populations. We find that multiple formulations of density dependence perform comparably at the detection, validation and projection stages of analysis. However, we tentatively recommend using a log‐linear or Weibull distribution to describe density‐dependent regulation of seabird breeding success in impact assessments to balance accuracy with caution. Finally, we show that relative PVA metrics of impact assessment cannot necessarily be used to overcome PVA misspecification by assuming density independence in positively regulated populations.

Synthesis and applications: We suggest that a density‐dependent approach when performing PVA‐based assessments for seabird populations will prevent biologically unrealistic, unconstrained population growth and therefore ensure meaningful PVA metrics in populations experiencing negative regulation. It will also maintain a precautionary approach for populations experiencing positive regulation, crucial when estimating impacts for these more vulnerable populations. These conclusions have immediate international application within the consenting processes for marine industries.
Making organ donation after circulatory death routine: Preserving patienthood and reproducing ways of dying in the intensive care unit
Authors: Cooper Jessie , Murphy Zivarna 
Published: August 2024

AbstractControlled organ donation after circulatory death (DCD) was re‐introduced in the UK in 2008, in efforts to increase rates of organs for transplant. Following reintroduction, there were debates about the ethics of DCD, including whether potential DCD donors receive end‐of‐life care which is in their best interests. Since this time, DCD has become a routine donor pathway in the NHS. In this article, we present findings from an ethnographic study examining the everyday practices of DCD in two English Trusts. Drawing on the concept of death brokering and Bea’s (2020) call to consider organ donation as embedded and routine practice within health care, we look at how DCD is integrated into end‐of‐life care in intensive care units. We show how DCD is made routine at the end‐of‐life via the practices of health professionals who create an active separation between discussions about death and donation; reproduce usual ways of doing things in end‐of‐life care; and respect the distinction between patient/donor, dying and death. In doing so, we argue these function to preserve the patienthood of the potential donor, ensuring DCD operates as an integrated part, and culturally accepted form of, good end‐of‐life care for potential donors, their relatives, and health professionals alike.