bluuebunny/crossref_metadata_2025_split huggingface dataset:
SRG/ART-XC Galactic Bulge deep survey. I. Maximum likelihood source detection algorithm for X-ray surveys
Authors: Semena A , Vikhlinin A , Mereminskiy I , Lutovinov A , Tkachenko A , Lapshov I , Burenin R 
Published: July 2024

ABSTRACT
We describe an X-ray source detection method entirely based on the maximum likelihood analysis, in application to observations with the ART-XC telescope onboard the Spectrum Roentgen Gamma observatory. The method optimally combines the data taken at different conditions, a situation commonly found in scanning surveys or mosaic observations with a telescope with a significant off-axis PSF distortion. The method can be naturally extended to include additional information from the X-ray photon energies, detector grades, etc. The likelihood-based source detection naturally results in an optimal use of available information for the sources detection and stable and uniform definition of detection thresholds under different observing conditions (PSF, background level). This greatly simplifies the statistical calibration of the survey needed to, e.g. obtain the $\log N - \log S$ distribution of detected sources or their luminosity function. The method can be applied to the data from any imaging X-ray telescope.
Spin parity of spiral galaxies. IV. Differential reddening of globular cluster systems of nearby spiral galaxies
Authors: Iye Masanori , Yagi Masafumi 
Published: July 2024

Abstract
The north-west side of the disk of M 31 is known as the near side because of the differential reddening of globular clusters found from their photographic photometry. This paper reports a simple geometric model to evaluate the visibility of the effect and its application to published CCD photometry on globular cluster systems of three spiral galaxies, M 31, M 33, and NGC 253. The color difference of globular cluster systems due to differential reddening was confirmed for M 31 and NGC 253; however, the data for M 33 were insufficient. The analysis reaffirms the currently adopted interpretation that the side on the minor axis of the galactic disk, where more conspicuous dust features and interstellar reddening are visible, is the nearer side to us and provides an additional basis for using spiral galaxies with identified spiral windings, S-wise or Z-wise, to study the large-scale spin distribution of galaxies in the universe.
The BNB–GLID module regulates germline fate determination in Marchantia polymorpha
Authors: Ren Xiaolong , Zhang Xiaoxia , Qi Xiaotong , Zhang Tian , Wang Huijie , Twell David , Gong Yu , Fu Yuan , Wang Baichen , Kong Hongzhi , Xu Bo 
Published: July 2024

Abstract
Germline fate determination is a critical event in sexual reproduction. Unlike animals, plants specify the germline by reprogramming somatic cells at the late stages of their development. However, the genetic basis of germline fate determination and how it evolved during the land plant evolution are still poorly understood. Here, we report that the plant homeodomain finger protein GERMLINE IDENTITY DETERMINANT (GLID) is a key regulator of the germline specification in liverwort, Marchantia polymorpha. Loss of the MpGLID function causes failure of germline initiation, leading to the absence of sperm and egg cells. Remarkably, the overexpression of MpGLID in M. polymorpha induces the ectopic formation of cells with male germline cell features exclusively in male thalli. We further show that MpBONOBO (BNB), with an evolutionarily conserved function, can induce the formation of male germ cell-like cells through the activation of MpGLID by directly binding to its promoter. The Arabidopsis (Arabidopsis thaliana) MpGLID ortholog, MALE STERILITY1 (AtMS1), fails to replace the germline specification function of MpGLID in M. polymorpha, demonstrating that a derived function of MpGLID orthologs has been restricted to tapetum development in flowering plants. Collectively, our findings suggest the presence of the BNB–GLID module in complex ancestral land plants that has been retained in bryophytes, but rewired in flowering plants for male germline fate determination.
The phosphatidylethanolamine-binding proteins OsMFT1 and OsMFT2 regulate seed dormancy in rice
Authors: Shen Jun , Zhang Liang , Wang Huanyu , Guo Jiazhuo , Li Yuchen , Tan Yuanyuan , Shu Qingyao , Qian Qian , Yu Hao , Chen Ying , Song Shiyong 
Published: July 2024

Abstract
Seed dormancy is crucial for optimal plant life-cycle timing. However, domestication has largely diminished seed dormancy in modern cereal cultivars, leading to challenges such as preharvest sprouting (PHS) and subsequent declines in yield and quality. Therefore, it is imperative to unravel the molecular mechanisms governing seed dormancy for the development of PHS-resistant varieties. In this study, we screened a mutant of BASIC HELIX-LOOP-HELIX TRANSCRIPTION FACTOR4 (OsbHLH004) with decreased seed dormancy and revealed that OsbHLH004 directly regulates the expression of 9-CIS-EPOXYCAROTENOID DIOXYGENASE3 (OsNCED3) and GIBBERELLIN 2-OXIDASE6 (OsGA2ox6) in rice (Oryza sativa). Additionally, we determined that two phosphatidylethanolamine-binding proteins, MOTHER OF FT AND TFL1 and 2 (OsMFT1 and OsMFT2; hereafter OsMFT1/2) interact with OsbHLH004 and Ideal Plant Architecture 1 (IPA1) to regulate their binding capacities on OsNCED3 and OsGA2ox6, thereby promoting seed dormancy. Intriguingly, FT-INTERACTING PROTEIN1 (OsFTIP1) interacts with OsMFT1/2 and affects their nucleocytoplasmic translocation into the nucleus, where OsMFT1/2–OsbHLH004 and OsMFT1/2–IPA1 antagonistically modulate the expression of OsNCED3 and OsGA2ox6. Our findings reveal that OsFTIP1-mediated inhibition of nuclear translocation of OsMFT1/2 and the dynamic transcriptional modulation of OsNCED3 and OsGA2ox6 by OsMFT1/2–OsbHLH004 and OsMFT1/2–IPA1 complexes in seed dormancy in rice.
Calcium-activated potassium channels as amplifiers of TRPV4-mediated pulmonary edema formation in male mice
Authors: Li Mei , Roeder Juliana , Blázquez-Prieto Jorge , Schulz Sabrina , Naujox Julia , Falivene Juliana , Erfinanda Lasti , Liedtke Wolfgang , Albaiceta Guillermo M. , Kuebler Wolfgang M. , Michalick Laura 
Published: July 2024

Background:
As a mechanosensitive cation channel and key regulator of vascular barrier function, endothelial transient receptor potential vanilloid-type 4 (TRPV4) contributes critically to ventilator-induced lung injury (VILI) and edema formation. Ca2+ influx via TRPV4 can activate Ca2+-activated K + (KCa) channels, categorized into small (SK1-3), intermediate (IK1), and big (BK) KCa, which may in turn amplify Ca2+ influx by increasing the electrochemical Ca2+ gradient and thus, promote lung injury. We therefore hypothesized that endothelial KCa channels may contribute to the progression of TRPV4-mediated VILI.

Methods:
Male C57Bl/6J mice were ventilated for 2 h with low or high tidal volumes in the presence or absence of the non-selective KCa antagonists apamin, charybdotoxin, or the selective IK1 antagonist TRAM34. Lung injury was similarly assessed in overventilated, endothelial-specific TRPV4-deficient mice or TRAM34-treated C57Bl/6J mice challenged with intratracheal acid installation. Changes in endothelial Ca2+ concentration ([Ca2+]i) were monitored by real-time imaging in isolated-perfused lungs in response to airway pressure elevation or in human pulmonary microvascular endothelial cells (HPMECs) in response to TRPV4 activation with or without inhibition of KCa channels. Analogously, changes in intracellular potassium concentration ([K+]i) and membrane potential (V
m) were imaged in vitro.

Results:
Endothelial TRPV4 deficiency or inhibition of KCa channels, and most prominently inhibition of IK1 by TRAM34 attenuated VILI as demonstrated by reduced lung edema, protein leak, and by quantitative lung histology. All KCa antagonists reduced the [Ca2+]i response to mechanical stimulation or direct TRPV4 activation in isolated lungs. TRAM34 and charybdotoxin, yet not apamin prevented TRPV4-induced K+ efflux and membrane hyperpolarization in HPMECs. TRAM34 also attenuated the TRPV4 agonist-induced Ca2+ influx in vitro and reduced acid-induced lung injury in vivo.

Conclusions:
KCa channels, specifically IK1, act as amplifiers of TRPV4-mediated Ca2+ influx and establish a detrimental feedback that promotes barrier failure and drives the progression of VILI.
Heparin-Induced Thrombocytopenia in Patients Suffering Cardiogenic Shock
Authors: Lüsebrink Enzo , Lanz Hugo , Binzenhöfer Leonhard , Hoffmann Sabine , Höpler Julia , Kraft Marie , Gade Nils , Gmeiner Jonas , Roden Daniel , Saleh Inas , Hagl Christian , Nickenig Georg , Massberg Steffen , Zimmer Sebastian , Jamin Raúl Nicolás , Scherer Clemens 
Published: July 2024

OBJECTIVES:
Cardiogenic shock (CS) is associated with high mortality. Patients treated for CS mostly require heparin therapy, which may be associated with complications such as heparin-induced thrombocytopenia (HIT). HIT represents a serious condition associated with platelet decline and increased hypercoagulability and remains a poorly researched field in intensive care medicine. Primary purpose of this study was to: 1) determine HIT prevalence in CS, 2) assess the performance of common diagnostic tests for the workup of HIT, and 3) compare outcomes in CS patients with excluded and confirmed HIT.

DESIGN:
Retrospective dual-center study including adult patients 18 years old or older with diagnosed CS and suspected HIT from January 2010 to November 2022.

SETTING:
Cardiac ICU at the Ludwig-Maximilians University hospital in Munich and the university hospital of Bonn.

PATIENTS AND INTERVENTIONS:
In this retrospective analysis, adult patients with diagnosed CS and suspected HIT were included. Differences in baseline characteristics, mortality, neurologic and safety outcomes between patients with excluded and confirmed HIT were evaluated.

MEASUREMENTS AND MAIN RESULTS:
In cases of suspected HIT, positive screening antibodies were detected in 159 of 2808 patients (5.7%). HIT was confirmed via positive functional assay in 57 of 2808 patients, corresponding to a prevalence rate of 2.0%. The positive predictive value for anti-platelet factor 4/heparin screening antibodies was 35.8%. Total in-hospital mortality (58.8% vs. 57.9%; p > 0.999), 1-month mortality (47.1% vs. 43.9%; p = 0.781), and 12-month mortality (58.8% vs. 59.6%; p > 0.999) were similar between patients with excluded and confirmed HIT, respectively. Furthermore, no significant difference in neurologic outcome among survivors was found between groups (Cerebral Performance Category [CPC] score 1: 8.8% vs. 8.8%; p > 0.999 and CPC 2: 7.8% vs. 12.3%; p = 0.485).

CONCLUSIONS:
HIT was a rare complication in CS patients treated with unfractionated heparin and was not associated with increased mortality. Also, HIT confirmation was not associated with worse neurologic outcome in survivors. Future studies should aim at developing more precise, standardized, and cost-effective strategies to diagnose HIT and prevent complications.
Prenatal Exposure to Nonpersistent Chemicals and Fetal-to-childhood Growth Trajectories
Authors: Bommarito Paige A. , Blaauwendraad Sophia M. , Stevens Danielle R. , van den Dries Michiel A. , Spaan Suzanne , Pronk Anjoeka , Tiemeier Henning , Gaillard Romy , Trasande Leonardo , Jaddoe Vincent V.W. , Ferguson Kelly K. 
Published: July 2024

Introduction:
Prenatal exposure to nonpersistent chemicals, including organophosphate pesticides, phthalates, and bisphenols, is associated with altered fetal and childhood growth. Few studies have examined these associations using longitudinal growth trajectories or considering exposure to chemical mixtures.

Methods:
Among 777 participants from the Generation R Study, we used growth mixture models to identify weight and body mass index trajectories using weight and height measures collected from the prenatal period to age 13. We measured exposure biomarkers for organophosphate pesticides, phthalates, and bisphenols in maternal urine at three timepoints during pregnancy. Multinomial logistic regression was used to estimate associations between averaged exposure biomarker concentrations and growth trajectories. We used quantile g-computation to estimate joint associations with growth trajectories.

Results:
Phthalic acid (OR = 1.4; 95% CI = 1.01, 1.9) and bisphenol A (OR = 1.5; 95% CI = 1.0, 2.2) were associated with higher odds of a growth trajectory characterized by smaller prenatal and larger childhood weight relative to a referent trajectory of larger prenatal and average childhood weight. Biomarkers of organophosphate pesticides, individually and jointly, were associated with lower odds of a growth trajectory characterized by average prenatal and lower childhood weight.

Conclusions:
Exposure to phthalates and bisphenol A was positively associated with a weight trajectory characterized by lower prenatal and higher childhood weight, while exposure to organophosphate pesticides was negatively associated with a trajectory of average prenatal and lower childhood weight. This study is consistent with the hypothesis that nonpersistent chemical exposures disrupt growth trajectories from the prenatal period through childhood.
Ultrasound-guided transversalis fascia plane block or transversus abdominis plane block for recovery after caesarean section
Authors: Pinarbaşi Ahmet , Altiparmak Başak , Korkmaz Toker Melike , Pirinççi Fatih , Uğur Bakiye 
Published: July 2024

BACKGROUND
Caesarean section is a widely performed surgical procedure that often results in moderate-to-severe postoperative pain. If left untreated, this pain can lead to short-term and long-term consequences. Transversalis fascia plane (TFP) block and transversus abdominis plane (TAP) block are among the regional anaesthesia techniques employed for managing pain after a caesarean section.

OBJECTIVE
We aimed to compare the impact of these two blocks on the quality of recovery in patients undergoing elective caesarean section under spinal anaesthesia.

DESIGN
A single-centre, double-blind, randomised trial.

SETTINGS
Operating room, postanaesthesia recovery unit, and ward in a tertiary hospital.

PARTICIPANTS
Ninety-three patients (ASA 2 to 3) were recruited. After exclusion, 79 patients were included in the final analysis: 40 in the TFP block group and 39 in the TAP block group.

INTERVENTIONS
After surgery, participants received either TFP block (20 ml 0.25% bupivacaine for each side) or TAP block (20 ml 0.25% bupivacaine for each side).

MAIN OUTCOME MEASURES
The primary outcome was the difference in obstetric quality of recovery 11-Turkish (ObsQoR-11T) scores between groups. Secondary outcomes included pain scores, opioid consumption and incidence of opioid-related complications.

RESULTS
The mean ObsQoR-11T score was higher in the TFP block group compared with the TAP block group (97.13 ± 6.67 points vs. 87.10 ± 9.84 points, respectively; P < 0.001). The pain scores in the TFP block group were slightly lower between postoperative 4 and 24 h. The mean total morphine consumption was 15.08 ± 2.21 mg in the TFP block group and 22.21 ± 3.04 mg in the TAP block group (P < 0.001). More patients required rescue analgesia between 4 and 8 h in the TAP block group [2.00 (5.00%) vs. 9.00 (23.08%), P = 0.02]. No significant differences were observed between groups in terms of opioid-related side effects.

CONCLUSION
TFP block used for analgesic purposes yielded a better quality recovery period than TAP block and also reduced opioid consumption.

TRIAL REGISTRATION
Clinicaltrials.gov (NCT05999981).

VISUAL ABSTRACT

http://links.lww.com/EJA/B6.
Endothelial RUNX3 controls LSEC dysfunction and angiocrine LRG1 signaling to prevent liver fibrosis
Authors: Ojha Uttam , Kim Somi , Rhee Chang Yun , You Jihye , Choi Yoon Ha , Yoon Soo-Hyun , Park Soo Young , Lee Yu Rim , Kim Jong Kyoung , Bae Suk-Chul , Lee You Mie 
Published: July 2024

Background and Aims:
Liver fibrosis represents a global health burden, given the paucity of approved antifibrotic therapies. Liver sinusoidal endothelial cells (LSECs) play a major gatekeeping role in hepatic homeostasis and liver disease pathophysiology. In early tumorigenesis, runt-related transcription factor 3 (RUNX3) functions as a sentinel; however, its function in liver fibrosis in LSECs remains unclear. This study aimed to investigate the role of RUNX3 as an important regulator of the gatekeeping functions of LSECs and explore novel angiocrine regulators of liver fibrosis.

Approach and Results:
Mice with endothelial Runx3 deficiency develop gradual and spontaneous liver fibrosis secondary to LSEC dysfunction, thereby more prone to liver injury. Mechanistic studies in human immortalized LSECs and mouse primary LSECs revealed that IL-6/JAK/STAT3 pathway activation was associated with LSEC dysfunction in the absence of RUNX3. Single-cell RNA sequencing and quantitative RT-PCR revealed that leucine-rich alpha-2-glycoprotein 1 (LRG1) was highly expressed in RUNX3-deficient and dysfunctional LSECs. In in vitro and coculture experiments, RUNX3-depleted LSECs secreted LRG1, which activated HSCs throughTGFBR1–SMAD2/3 signaling in a paracrine manner. Furthermore, circulating LRG1 levels were elevated in mouse models of liver fibrosis and in patients with fatty liver and cirrhosis.

Conclusions:
RUNX3 deficiency in the endothelium induces LSEC dysfunction, LRG1 secretion, and liver fibrosis progression. Therefore, endothelial RUNX3 is a crucial gatekeeping factor in LSECs, and profibrotic angiocrine LRG1 may be a novel target for combating liver fibrosis.
Good therapeutic connections and patient psychological safety: A qualitative survey study
Authors: Rathert Cheryl , Simmons Derick R. , Mittler Jessica N. , Enard Kimberly , Brooks Joanna Veazey 
Published: July 2024

Background
Therapeutic connections (TCs) between patients and care providers are important for achieving desired patient outcomes. For patients, TC is associated with greater health self-efficacy, better health status, mental health status, and higher satisfaction with providers.

Purpose
The aim of the study was to examine patients’ descriptions of what signals to them they have a TC with their care provider.

Methodology
We conducted an online survey of patients with a recent health care visit (n = 1,766). This study analyzed the results of an open-ended question that asked how patients know they have a good TC with their care provider. Data were analyzed using framework analysis to determine the extent to which patient responses indicated TC dimensions. A thematic content analysis identified emergent themes.

Results
Of the TC dimensions, words associated with Shared Deliberation were mentioned by 60% of respondents. Other dimension mentions ranged between 14% (Shared Mind) and 2% (Bond). Thematic content analysis revealed that patient psychological safety seems to be required for many patients to feel connected.

Conclusion
A majority of patients indicated that good TCs happen when they feel seen and heard by providers. However, it appears that prior to feeling a strong TC, patients need to feel safe to be fully welcome into the encounter.

Practice Implications
Health care organizations need to give care providers the uninterrupted time and space they need to fully connect with patients. Training about how to create a psychologically safe environment for patients should be tailored for practicing providers, leaders, and students in health care fields.
Do Interventions Meet the Needs of Caregivers of Cardiac Patients?
Authors: Nissen Nina Konstantin , Guldin Mai-Britt , Nielsen Camilla Palmhøj , Ørtenblad Lisbeth 
Published: July 2024

Background
Interventions targeting the needs of caregivers of cardiac patients are few, and in most cases, no effect is found on caregivers' well-being. A closer look at the existing interventions will provide a solid foundation for future efforts to develop effective interventions targeted at caregivers of cardiac patients.

Objective
The study's objective was to scrutinize and discuss interventions targeting caregivers of cardiac patients and contribute to reflections that will improve future interventions.

Methods
Systematic scoping of the literature within the field was conducted through a literature search in PubMed, EMBASE, CINAHL, PsycINFO, and Cochrane Library from January 2011 to May 2022. The development of search terms and inclusion criteria was inspired by the Patient problem/population, Intervention, Comparison/Control, and Outcome approach, and the Medical Research Council framework for developing and evaluating complex interventions served as the underlying basis for the analysis.

Results
Eleven articles were included. The interventions reported in the articles were generally not systematically developed and did not include field-specific, methodological, and theoretical reflections. Furthermore, the development process behind the studies seemed not to be transparent.

Conclusions
Lack of systematic methodology and methodological transparency in the reviewed studies hinders further testing of interventions and might explain lack of evidence for effective interventions within the field. More systematic, needs-based, and well-documented interventions targeting caregivers of various kinds of cardiac patients are needed to develop the field to the benefit of caregivers, patients, and society.
Advancing Vaccine Uptake in People With HIV: A Call for Research on Trust and Intellectual Humility in Health Care
Authors: Barr Emily A. , Celniker Jared B. , Ballantyne Nathan 
Published: July 2024

Abstract
This article addresses the challenge of vaccine hesitancy among people with HIV (PWH), emphasizing the need for research on the potential impact of trust and intellectual humility in health care. It underscores the complexity of vaccine acceptance in PWH and the urgency of addressing hesitancy in PWH ahead of a future HIV vaccine. The article identifies trust in health care providers as a critical factor influencing vaccine uptake and proposes that providers who demonstrate intellectual humility—openly recognizing the limits of their knowledge—might enhance patient trust. The role of nurses is spotlighted because of their social positioning in the patient experience. The article advocates for interventions tailored to PWH's unique experiences and attitudes. Such strategies are essential for improving vaccine uptake and, consequently, public health outcomes.