bluuebunny/crossref_metadata_2025_split huggingface dataset:
HTB50-2 Inhibits Growth and Migration of Triple-negative Breast Cancer via FOSL2/FOXC1 Signaling Axis and Subsequent Ferroptosis
Authors: Liu Na , Yin Wen-ying , Duan Wen-qi , Lyu Yu-zhu , Xie Shan , Fang Lei , Ji Yan , Yang Feng-ying , Ge Di 
Published: August 2024

Background:
The manipulation of ferroptosis in cancer cells is a possible
therapeutic technique that has been investigated for use in the treatment of cancer.
Consequently, ferroptosis-inducing medications have recently received increased interest
in cancer therapy. In this research, we assessed the anticancer efficacy of 14β-hydroxy-
3β-(β-D-Glucopyranosyloxy)-5α-bufa-20,22-dienolide (HTB50-2), a natural product
derived from the plant Helleborus thibetanus Franch, in Triple-Negative Breast Cancer
(TNBC). Moreover, we also studied its potential mechanisms.

Methods:
The biological effects of HTB50-2 in a series of breast cancer cell lines were
analyzed using sulforhodamine B (SRB) and other methods. The migration ability was
analyzed using three methods: wound healing assay, transwell assay, and Western blot.
Meanwhile, the potential therapeutic value of HTB50-2 was evaluated in BALB/c mice
by orthotopic transplantation. Transcriptome sequencing was conducted to explore the
FOS-like antigen 2 (FOSL2) gene, and its role in ferroptosis was verified by Western
blot and immunohistochemistry. The association of FOSL2 and ferroptosis-related
genes was analyzed using NetworkAnalyst databases, and a TF-Gene interaction network
was constructed.

Results:
Ferroptosis was found to be induced in TNBC cells by HTB50-2. Furthermore,
HTB50-2 inhibited tumor development by inducing ferroptosis in TNBC in vivo. Mechanistically,
we demonstrated that a transcription factor FOSL2 mediated ferroptosis by
HTB50-2. Additionally, it was found that Forkhead box C1 (FOXC1) was regulated by
FOSL2 and correlated with ferroptosis.

Conclusion:
Our data suggest that HTB50-2 exerts its anti-cancer properties by ferroptosis
via FOSL2/FOXC1 signaling pathway. Hence, HTB50-2 has an important application
potential in the treatment of TNBC.
Weight, CYP3A5 Genotype, and Voriconazole Co-administration Influence Tacrolimus Initial Dosage in Pediatric Lung Transplantation Recipients with Low Hematocrit based on a Simulation Model
Authors: Hu Ke , Pan Jia-Jun , Qu Wen-Qian , He Su-Mei , Yang Yang , Shi Hao-Zhe , Zhang Yi-Jia , Chen Xiao , Wang Dong-Dong 
Published: August 2024

Objective:
The method of administering the initial doses of tacrolimus in recipients of pediatric
lung transplantation, especially in patients with low hematocrit, is not clear. The present study aims to explore
whether weight, CYP3A5 genotype, and voriconazole co-administration influence tacrolimus initial dosage in
recipients of pediatric lung transplantation with low hematocrit based on safety and efficacy using a simulation
model.

Methods:
The present study utilized the tacrolimus population pharmacokinetic model, which was employed
in lung transplantation recipients with low hematocrit.

Results:
For pediatric lung transplantation recipients not carrying CYP3A5*1 and without voriconazole, the recommended
tacrolimus doses for weights of 10-13, 13-19, 19-22, 22-35, 35-38, and 38-40 kg are 0.03, 0.04,
0.05, 0.06, 0.07, and 0.08 mg/kg/day, which are split into two doses, respectively. For pediatric lung transplantation
recipients carrying CYP3A5*1 and without voriconazole, the recommended tacrolimus doses for
weights of 10-18, 18-30, and 30-40 kg are 0.06, 0.08, 0.11 mg/kg/day, which are split into two doses, respectively.
For pediatric lung transplantation recipients not carrying CYP3A5*1 and with voriconazole, the recommended
tacrolimus doses for weights of 10-20 and 20-40 kg are 0.02 and 0.03 mg/kg/day, which are split into
two doses, respectively. For pediatric lung transplantation recipients carrying CYP3A5*1 and with voriconazole,
the recommended tacrolimus doses for weights of 10-20, 20-33, and 33-40 kg are 0.03, 0.04, and 0.05
mg/kg/day, which are split into two doses, respectively.

Conclusion:
The present study is the first to recommend the initial dosages of tacrolimus in recipients of pediatric
lung transplantation with low hematocrit using a simulation model.
Expression and Function of FAM72A Gene in Multiple MyelomaFAM72A
Authors: Gao Wenyu , Ma Yanping 
Published: August 2024

Aims:
This study aims to comprehensively investigate the role of Family Member A
with sequence similarity 72-A (FAM72A) in multiple myeloma.

Background:
Multiple myeloma poses significant challenges. This study delves into FAM72A's
impact on key cellular processes, shedding light on potential therapeutic targets and enhancing
our understanding of multiple myeloma progression.

Objective:
Investigate the impact of FAM72A on the proliferation, apoptosis, and bortezomib
sensitivity of multiple myeloma cell line U266.

Methods:
qRT-PCR analyzed FAM72A expression levels in bone marrow samples from 30 patients
with multiple myeloma and 10 healthy donors at the Second Hospital of Shanxi Medical
University. Cell lines overexpressing FAM72A were constructed, and Cell Counting Kit 8
(CCK-8) and flow cytometry were used to assess U266 cell proliferation, apoptosis, and sensitivity
to bortezomib. Biological predictions for FAM72A were performed to find transcription factors
binding to the FAM72A promoter region, verified using a luciferase assay. U266 cells were
transfected with si-POU2F2 (POU class 2 homeobox 2), and the impact on cell proliferation was
validated. Western blot analysis detected the expression of downstream proteins in the p53 signaling
pathway. In vivo, experiments established a xenograft mouse model further to study the
role of FAM72A in multiple myeloma.

Results:
FAM72A was upregulated in multiple myeloma bone marrow tissues. Compared to the
OE-NC group, the OE-FAM72A group showed increased Mouse Double Minute 2 homolog
(MDM2) expression, decreased p53 expression, increased cell proliferation, and decreased apoptosis.
POU2F2 was identified as the upstream transcription factor for FAM72A. Compared to the
si-NC group, the si-POU2F2 group exhibited decreased MDM2 expression, increased p53 expression,
slowed cell proliferation, and increased apoptosis. Silencing POU2F2 could reverse the
pro-proliferative effect of over-expressing FAM72A in U266 cells. In vivo experiments in a xenograft
mouse model further studied the role of FAM72A in multiple myeloma.

Conclusion:
Overexpression of FAM72A promotes U266 cell proliferation, inhibits apoptosis,
and reduces sensitivity to bortezomib by regulating the POU2F2/FAM72A/p53 signaling pathway.
Mining Transcriptional Data for Precision Medicine: Bioinformatics Insights into Inflammatory Bowel Disease
Authors: Shahriari Arman , Amirzadeh Shams Shokoofeh , Mahboobi Hamidreza , Yazdanparast Maryam , Jabbaripour Sarmadian Amirreza 
Published: August 2024

Abstract:
Inflammatory Bowel Disease (IBD), encompassing ulcerative colitis and Crohn’s disease,
affects millions worldwide. Characterized by a complex interplay of genetic, microbial, and
environmental factors, IBD challenges conventional treatment approaches, necessitating precision
medicine. This paper reviews the role of bioinformatics in leveraging transcriptional data for novel
IBD diagnostics and therapeutics. It highlights the genomic landscape of IBD, focusing on genetic
factors and insights from genome-wide association studies. The interrelation between the gut microbiome
and host transcriptional responses in IBD is examined, emphasizing the use of bioinformatics
tools in deciphering these interactions. Our study synthesizes developments in transcriptomics
and proteomics, revealing aberrant gene and protein expression patterns linked to IBD pathogenesis.
We advocate for the integration of multi-omics data, underscoring the complexity and necessity
of bioinformatics in interpreting these datasets. This approach paves the way for personalized
treatment strategies, improved disease prognosis, and enhanced patient care. The insights provided
offer a comprehensive overview of IBD, highlighting bioinformatics as key in advancing personalized
healthcare in IBD management.
IDENTIFIKASI PERENCANAAN FASILITAS UMUM SARANA PERMUKIMAN DI KECAMATAN KARANGMONCOL KABUPATEN PURBALINGGA
Authors: Ahmada Naufal , Priambodo Aji 
Published: August 2024

Penelitian ini adalah analisis proyeksi penduduk dengan analisis ketersediaan dan kebutuhan fasilitas menggunakan Standar Nasional Indonesia (SNI 03-1733-200) di Kecamatan Karangmoncol Kabupaten Purbalingga. Penelitian ini bertujuan untuk identifikasi kondisi terkini terkait jumlah penduduk terhadap sebaran fasilitas umum dan analisis kebutuhan fasilitas umum, serta proyeksi pada 5 dan 10 tahun kedepan. Penelitian ini menggunakan metode analisis deskriptif dengan pendekatan kualitatif. Variabel yang digunakan adalah variabel penduduk terhadap variabel pendidikan, kesehatan, peribadatan, perdagangan, dan persampahan. Hasil penelitian adalah sarana pendidikan tingkat SMA masih terpusat di Pekiringan, Tamansari, Tunjungmuli yang menyebabkan adanya kesenjangan di wilayah lain dimana belum adanya SMA dan tingkatan SMP dimana hanya Grantung yang masih belum ada sehingga harus ada penambahan SMP. Sarana kesehatan puskesmas hanya ada di Pepedan saja yang juga menyebabkan kesenjangan di wilayah lain. Sarana peribadatan sudah mencakup dan melayani seluruh kelurahan di Kecamatan Karangmoncol sehingga dianggap sudah memenuhi standar. Sarana perdagangan dianggap sudah memenuhi standar. Sarana persampahan berupa TPS sudah memenuhi standar, sebaiknya perlu ditambahkan TPA.
Identifying Barriers to the Adoption of Digital Contact Tracing Apps in England: Semistructured Interview Study With Professionals Involved in the Pandemic Response
Authors: Palmer Anna , Sharma Shaishab , Nagpal Jayesh , Kimani Victor , Mai Florence , Ahmed Zara 
Published: August 2024

Background
The NHS (National Health Service) COVID-19 app was a digital contact tracing app (DCTA) used in England in response to the COVID-19 pandemic. The aim of which was to limit the spread of COVID-19 by providing exposure alerts. At the time of the pandemic, questions were raised regarding the effectiveness and cost of the NHS COVID-19 app and whether DCTAs have a role in future pandemics.

Objective
This study aims to explore key barriers to DCTAs in England during the COVID-19 pandemic.

Methods
This is a qualitative study using semistructured video interviews conducted with professionals in public health, digital health, clinicians, health care law, and health executives who had an active role in the COVID-19 pandemic. These interviews aimed to explore the perspective of different experts involved in the pandemic response and gauge their opinions on the key barriers to DCTAs in England during the COVID-19 pandemic. The initial use of maximum variation sampling combined with a snowball sampling approach ensured diversity within the cohort of interviewees. Interview transcripts were then analyzed using Braun and Clarke's 6 steps for thematic analysis.

Results
Key themes that acted as barriers to DCTAs were revealed by interviewees such as privacy concerns, poor communication, technological accessibility, digital literacy, and incorrect use of the NHS COVID-19 app. Interviewees believed that some of these issues stemmed from poor governmental communication and a lack of transparency regarding how the NHS COVID-19 app worked, resulting in decreased public trust. Moreover, interviewees highlighted that a lack of social support integration within the NHS COVID-19 app and delayed app notification period also contributed to the poor adoption rates.

Conclusions
Qualitative findings from interviews highlighted barriers to the NHS COVID-19 app, which can be applied to DCTAs more widely and highlight some important implications for the future use of DCTAS. There was no consensus among interviewees as to whether the NHS COVID-19 app was a success; however, all interviewees provided recommendations for improvements in creating and implementing DCTAs in the future.
A Comparative Study of Yuanti Flower-and-bird Paintings and Western Classical Still-life Paintings: A Formal and Thematic Analysis of Li Song’s “Basket of Flowers” and Caravaggio’s “Basket of Fruit”
Authors: Lin Shuxin 
Published: August 2024

In the context of globalization, cultural exchange between the East and West is becoming increasingly frequent, and painting serves as a unique perspective for exploring cultural differences. In particular, flower-and-bird paintings and still-life paintings, as two major traditional painting genres, share similarities in selecting the subject and pursuing realism while maintaining their artistic characteristics, which both act as effective research models for cross-cultural comparative studies. This paper selects Li Song’s “Basket of Flowers”, a representative of Yuanti flower-and-bird paintingsy, and Caravaggio’s “Basket of Fruit”, an iconic one of the 17th-century Western classical still-life paintings. Through visual interpretation and cultural tracing, it analyzes the differences between the two in terms of color application, spatial organization, modelling techniques, and artistic spirit, to reveal the heterogeneity of floral subjects in Yuanti flower-and-bird paintings and Western classical still-life paintings, and to examine the different artistic values and humanistic spirits manifested in Chinese and Western paintings.
Ácidos grasos y terpenos del extracto metanólico de Artemisia cina como posibles responsables del efecto ovicida sobre Haemonchus contortus
Authors: Arango-De-la-Pava Luis David , De la Cruz-Cruz Héctor Alejandro , Cuéllar-Ordaz Jorge Alfredo , Zamilpa Alejandro , González-Cortázar Manasés , López-Arellano María Eugenia , Higuera-Piedrahita Rosa Isabel , López-Arellano Raquel 
Published: August 2024

Haemonchus contortus es un nematodo hematófago con alta tasa de reproducción, considerado como el principal problema en pequeños rumiantes en pastoreo. Por tanto, se buscan alternativas de tratamiento con base en el uso de los extractos vegetales. El objetivo de este estudio fue evaluar la actividad ovicida de Artemisia cina contra el parásito Haemonchus contortus y caracterizar químicamente el extracto con mayor actividad biológica a través de cromatografía de gases acoplado a espectrometría de masas (CG-MS). La obtención de los extractos a evaluar, se realizó con la técnica de maceración utilizando metanol, acetato de etilo y n-hexano. Los extractos se llevaron a sequedad total y se desafiaron frente a los huevos de H. contortus utilizando la técnica de inhibición de la eclosión de huevos descrita por la Asociación Mundial para el Avance de la Parasitología Veterinaria (WAAVP, por sus siglas en inglés). El extracto metanólico (EM) mostró el 100 % de actividad ovicida a una concentración de 4.25 mg/ml, siendo el más activo a una baja concentración, por tanto se caracterizó con CG-MS. EL EM posee principalmente ácidos grasos y terpenos; de ellos, el ácido hexadecanoico y el 2-[4-metil-6-(2,6,6-trimetilciclohex-1-enil) exa-1,3,5-trienil][ciclohex]-1-en-carboxialdehido. Los compuestos caracterizados han mostrado actividad antihelmíntica previamente reportada, por lo que la actividad ovicida puede estar asociada a estos. En conclusión, el extracto metanólico de A. cina tuvo una mayor actividad ovicida a bajas concentraciones, esto se debe probablemente a la presencia de ácidos grasos y terpenos.
Social infrastructure of rural territories as a potential for agricultural development in the Nizhny Novgorod region
Authors: Minina Natalya , Shamina Olga , Gruzdev Georgiy 
Published: August 2024

Introduction. The article discusses the main problems that hinder the development of rural areas. An assessment of the social infrastructure of rural areas of the Nizhny Novgorod region was also carried out and the trends of their development were identified.Materials and methods. In this work, data materials from the territorial body of the Federal State Statistics Service of the Nizhny Novgorod region were used.Results. The result of the work is an analysis of the current state of rural areas and proposals for their development. The following methods were used and applied in the study: monographic, abstract-logical, economic-statistical, observations, comparisons.Discussion. During the analysis, the main reasons that slow down progress in the development of rural areas were identified: limited information, a shortage of labor (highly skilled personnel), limited funds for the development of territories, the geographical location of territories relative to urban settlements. To solve these problems, it is necessary to carry out comprehensive measures, including increasing financing for infrastructure development, attracting qualified personnel to work on projects, improving access to relevant information and creating conditions for project implementation.Conclusion. To date, the issue of increasing the density of the population living in rural areas is particularly acute and is a priority in the development of the country. As well as improving health and quality of life, ensuring social protection of citizens of the Russian Federation. To achieve the set strategic goals, programs are being implemented to support rural areas, measures are being taken to improve the quality of life of the rural population, working conditions, health care, etc.
Technology Coopetition and Voluntary Disclosures of Innovation
Authors: Oh Jun , Yeung P. Eric , Zhu Bo 
Published: August 2024

ABSTRACT
We examine firms’ voluntary disclosures of innovation under technology coopetition, focusing on technology standard setting organizations (SSOs). Technology coopetition is characterized by (1) cooperation to determine technology standards, which requires information sharing to reach consensus, and (2) competition for standard implementation to obtain standard-essential patents, which create incentives for firms to deviate from the expected level of information sharing. We document a decrease in 10-K narrative R&D disclosures, more generic 10-K narrative R&D disclosures, and a longer delay of patent disclosures via the USPTO after a firm joins an SSO. Among alternative explanations, our evidence is most supportive of the hypothesis that firms strategically withhold innovation information.
JEL Classifications: L15; M41; O32.
Improved Diabetes Screening for Women After Gestational Diabetes Mellitus
Authors: Strelow Brittany , Herndon Justine , McMahon AnneMarie , Takagi Mark , McCoy Rozalina , Olson Rachel , O’Laughlin Danielle 
Published: August 2024

OBJECTIVE
This study aimed to assess the need for practice-wide quality improvement to support evidence-based type 2 diabetes screening for women with a history of gestational diabetes mellitus (GDM) receiving primary care. We sought to add the diagnosis of GDM to the problem list of women who did not have it at baseline.

RESEARCH DESIGN AND METHODS
We identified all women in our practice with a history of GDM diagnosed between 2002 and 2023, quantified the proportion with GDM documented in their problem list, and examined patient- and clinician-level factors associated with having GDM appropriately documented at baseline.

RESULTS
We identified 203 women with GDM receiving primary care within internal medicine. Of the 203 women, 73 (35.0%) did not have GDM documented in their problem list. Of those without GDM included on the problem list, 52% were overdue for type 2 diabetes screening compared with 41% of those with GDM documented before our intervention. We found race, parity, and previous abnormal glycemic laboratory test results to be highly predictive of whether the history of GDM was on patients’ problem list. Upon completion of our intervention, we successfully achieved a 100% documentation rate for GDM diagnosis for women who previously lacked documentation in their problem list.

CONCLUSION
This work paves the way for targeted interventions aimed at improving care for women with a history of GDM, including delivery of interventions and education to prevent the onset of an appropriate clinical screening for type 2 diabetes.
Thermoplastic Starch Film Made from Cellulose to Extend the Shelf Life of Red Chilies
Authors: Sari Vonny Indah , Rahmah Anania , Putri Vivin Jenika , Azzahro Hanifah Ulfa , Nasution Lukman Hakim 
Published: August 2024

Thermoplastic starch (TPS) is a biofilm made from starch and cellulose. The advantage of thermoplastic starch is easily degraded. TPS has weaknesses in high water affinity and low mechanical properties, so it is necessary to add polyvinyl alcohol (PVA) in making TPS packaging. The mechanical properties of TPS-PVA can be further improved by modifying it into a composite with coconut fiber cellulose. This research was purposed to produces a film containing cellulose from coconut fiber as a reinforcing material for film composites as well as to evaluate the biodegradation properties of the resulting film. It is known that the addition of 5% w/v coconut cellulose fiber is the formulation with the best yield when compared with the 1% and 3% w/v formulations. Applying TPS film to chilies can increase the shelf life of chilies. As a product that spoils quickly, one of the efforts made to maintain the quality of chilies is to use packaging made from TPS. Storing chilies at different temperature conditions using TPS-PVA film packaging with the addition of 5% w/v coconut fiber cellulose was able to maintain the shelf life and quality of chilies for 19 days at cold temperatures. Keywords: Chilies, Cellulose, Thermoplastic starch
Walking Together: Dynamics of Muslim Wives Dual Role in Rural Areas Pursuing Career and Household Responsibilities
Authors: Saiin Asrizal , Firdaus Firdaus , Yusuf Maulana , Supani Supani , Muchimah Muchimah , Radiamoda Anwar M. 
Published: August 2024

The social reality today in rural areas, a wife works to help the family economy, besides having to do domestic tasks. The concept of gender does not prohibit a wife from working. However, this will be a problem if it creates gender inequality. This study aims to reveal the problem of fulfilling domestic duties for career wives in terms of gender perspective. This research is qualitative with the type of literature study, descriptive analytical method with based on a gender approach. The result of this study is that the career wife in fulfilling domestic duties in rural areas based on a gender perspective can be seen from the role that must be carried out. The wife's roles in a gender perspective are: a) mother's role, b) wife's role, c) social role, d) family's motivator. Getting many roles, apart from being a housewife and also having to work is not an easy thing for a wife. This role must be shared with the husband so that it can be balanced so as to create harmony in a household. A woman who already has a role as a mother, wife, member of the community and also has to play a role in the family, but the husband must help and support fully.