bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 cell surface hydrophobicity and survival of the cells under adverse environmental conditions
Authors: Shakirova Laisana , Grube Mara , Gavare Marita , Auzina Lilija , Zikmanis Peteris 
Published: October 2012

Abstract
Changes in the cell surface hydrophobicity (CSH) of probiotic bacteria Lactobacillus acidophilus La5 and Bifidobacterium lactis Bb12 and the survival of these cells were examined in response to varied cultivation conditions and adverse environmental conditions. An inverse linear relationship (P &lt; 0.01) was detected between the CSH of intact L. acidophilus La5 and B. lactis Bb12 and survival of cells subjected to subsequent freezing/thawing, long-term storage or exposure to mineral and bile acids. The observed relationships were supported by significant correlations between the CSH and changes in composition of the cell envelopes (proteins, lipids and carbohydrates) of L. acidophilus La5 and B. lactis Bb12 examined using FT-IR spectroscopy and conventional biochemical analysis methods. The results also suggest that the estimates of hydrophobicity, being a generalized characteristic of cell surfaces, are important parameters to predict the ability of intact probiotic bacteria to endure extreme environments and therefore should be monitored during cultivation. A defined balance of cell components, which can be characterized by the reduced CSH values, apparently helps to ensure the resistance, improved viability and hence the overall probiotic properties of bacteria.
The origin and genetic variability of the Czech sika deer population
Authors: Barančeková M. , Krojerová‐Prokešová J. , Voloshina I. V. , Myslenkov A. I. , Kawata Y. , Oshida T. , Lamka J. , Koubek P. 
Published: October 2012

AbstractSika deer (Cervus nippon), native to Asia, formed two well‐established free‐living populations in the Czech Republic over the last century and continue to spread. Sika are also maintained in a large number of enclosures; these continue to introduce new individuals from the places of its origin as well as from other European countries. Despite extensive research into the morphology and ethology of the Czech sika deer, conducted over the last three decades, no study using genetic methods has been done. This study aimed to determine the genetic variability and the geographic origin of the Czech sika deer population. Two mitochondrial markers, the cytochrome b and the control region were analyzed in this study. Analysis of the two markers confirmed that the founder individuals of the Czech population originated from both native island (Japanese Islands) and native mainland (Far East Russia) populations. Results showed that the genetic variability of the Czech sika deer population is lower than the variability of the native Japanese population, but higher than that of the sampled part of the native Russian population. Also, the genetic variability was found to be higher within the samples from enclosures.
MicroRNA Regulation in Extreme Environments: Differential Expression of MicroRNAs in the Intertidal Snail Littorina Littorea During Extended Periods of Freezing and Anoxia
Authors: Biggar Kyle K. , Kornfeld Samantha F. , Maistrovski Yulia , Storey Kenneth B. 
Published: October 2012

Abstract
Several recent studies of vertebrate adaptation to environmental stress have suggested roles for microRNAs (miRNAs) in regulating global suppression of protein synthesis and/or restructuring protein expression patterns. The present study is the first to characterize stress-responsive alterations in the expression of miRNAs during natural freezing or anoxia exposures in an invertebrate species, the intertidal gastropod Littorina littorea. These snails are exposed to anoxia and freezing conditions as their environment constantly fluctuates on both a tidal and seasonal basis. The expression of selected miRNAs that are known to influence the cell cycle, cellular signaling pathways, carbohydrate metabolism and apoptosis was evaluated using RT-PCR. Compared to controls, significant changes in expression were observed for miR-1a-1, miR-34a and miR-29b in hepatopancreas and for miR-1a-1, miR-34a, miR-133a, miR-125b, miR-29b and miR-2a in foot muscle after freezing exposure at −6 °C for 24 h (P  &lt; 0.05). In addition, in response to anoxia stress for 24 h, significant changes in expression were also observed for miR-1a-1, miR-210 and miR-29b in hepatopancreas and for miR-1a-1, miR-34a, miR-133a, miR-29b and miR-2a in foot muscle (P  &lt; 0.05). Moreover, protein expression of Dicer, an enzyme responsible for mature microRNA processing, was increased in foot muscle during freezing and anoxia and in hepatopancreas during freezing. Alterations in expression of these miRNAs in L. littorea tissues may contribute to organismal survival under freezing and anoxia.
Modeling and simulations of high-power microwave devices using the CHIPIC code
Authors: ZHOU JUN , LIU D. G. , LIAO C. 
Published: October 2012

AbstractThe CHIPIC code, a fully electromagnetic particle-in-cell (PIC) code for modeling and simulations of high-power microwave (HPM) devices, is introduced in this paper. It consists of a two-dimensional (2D) code and a three-dimensional (3D) code. The 2D code can model and simulate HPM devices with symmetric structure on 2D Cartesian, cylindrical and polar grids, while the 3D code can model and simulate HPM devices on 3D Cartesian and cylindrical grids. The fields are calculated using the finite-difference time-domain scheme, and the particles are described by the PIC scheme. Various types of boundary conditions have also been implemented for different kinds of applications. In addition, the 3D code is specifically designed for high-performance modeling and computing. It uses the message passing interface and the open specifications for multiprocessing (OpenMP) for parallelization. Its parallel design ensures that it is capable of efficiently executing on a variety of architectures. In order to allow efficient use of parallel architectures, it provides automated partitioning and dynamic load balancing. Even though this code is still in development, it has successfully simulated various real-world HPM experimental devices. Simulation results on some typical HPM devices by using the CHIPIC code are given, which agree well with those obtained from some well-known PIC codes. Direction for future work is also presented.
Welfare, Wealth and Poverty in Urban China: TheDibaoand Its Differential Disbursement
Authors: Solinger Dorothy J. , Hu Yiyang 
Published: October 2012

AbstractIn the broader social science literature, most studies of social protection investigate welfare in democracies, and at the national level, and typically assume that welfare is given in order to influence voting. This paper, to the contrary, considers social assistance in authoritarian China at the urban level. Its findings are compatible with an explanation that there are two dissimilar logics influencing the distributional decisions of lower-level administrators. That is, there appear to be two modes of social policy implementation, which vary with the fiscal capacity of a given city, as indicated by its average wage: Wealthier cities seem to prefer to push off the streets those viewed as unsuited to a modern city, therefore allocating a substantial proportion of their social assistance funds to them, in order to entice them to stay at home. On the other hand, poorer places seem to permit such people to work outside, in the hope that they will thus be better able to support themselves, thereby saving the city money. A data set from China's Ministry of Civil Affairs was used.
Local Energy Efficiency Policy Implementation in China: Bridging the Gap between National Priorities and Local Interests
Authors: Kostka Genia , Hobbs William 
Published: October 2012

AbstractThis paper analyses Chinese sub-national governments’ implementation strategies to meet national energy efficiency targets in the 11th Five-Year Plan (2006–2010). Previous research has focused on the ways governance practices and decision-making structures shape implementation outcomes, yet very little attention has been given to what strategies local leaders actually employ to bridge national priorities with local interests. To illustrate how local leaders work politically, this paper highlights specific implementation methods officials use to strengthen formal incentives and create effective informal incentives to comply with energy efficiency mandates. The analysis is drawn from 53 interviews conducted in June and July of 2010 in Shanxi, a major coal-producing and energy-intensive province. Findings suggest that local government leaders conform to national directives by “bundling” the energy efficiency policy with policies of more pressing local importance or by “bundling” their energy efficiency objectives with the interests of groups with significant political influence. Ultimately, sub-national government officials frame policies in ways that give them legitimacy at the local level.
Shifting Boundaries between the State and Society: Village Cadres as New Activists in Collective Petition
Authors: Wang Juan 
Published: October 2012

AbstractDespite the central government's efforts in reducing fiscal burdens on peasants through fiscal reforms in the early 2000s, collective petitions in rural China remain. Complementary to the arbitrary and weak government explanations of state–society conflict, this article reveals the role of village cadres as activists in collective petition. Drawn from extensive fieldwork, I argue that by reducing local government revenues and recentralizing fiscal autonomy to the county level, central fiscal reforms have unintentionally induced a new force of resistance: village cadres. Being disenfranchised from previous privileges, village cadres are now allies rather than adversaries of peasant petitions. This article advances existing literature on China's contentious politics in two ways. First, it recognizes a new group of activists whose savoir-faire improves peasant knowledge of the state capacity in containing state–society conflict. Second, it proposes a dynamic understanding of contentious politics by highlighting the shifting boundaries between the state and society.
HIV testing in black Africans living in England
Authors: RICE B. , DELPECH V. , SADLER K. E. , YIN Z. , ELFORD J. 
Published: October 2012

SUMMARYWe examined the uptake of HIV testing in black Africans living in England before the introduction of national testing guidelines. Analyses were conducted using data from an anonymous self-completed questionnaire linked to oral fluid samples to establish HIV status in black Africans attending community venues in England in 2004. Of 946 participants, 44% had ever been tested for HIV and 29% had been tested in the previous 24 months. Of those with undiagnosed HIV, 45% had previously had a negative HIV test. Almost a third of people tested in the UK had been at general practice. Uptake of HIV testing was not associated with perceived risk of HIV. These findings highlight the need for the implementation of national HIV testing guidelines in the UK, including the promotion of testing in general practice. Regular testing in black Africans living in the UK should be promoted regardless of their HIV test history.
Influenza-associated excess mortality in southern Brazil, 1980–2008
Authors: FREITAS F. T. M. , SOUZA L. R. O. , AZZIZ-BAUMGARTNER E. , CHENG P. Y. , ZHOU H. , WIDDOWSON M. A. , SHAY D. K. , OLIVEIRA W. K. , ARAUJO W. N. 
Published: October 2012

SUMMARYIn order to estimate influenza-associated excess mortality in southern Brazil, we applied Serfling regression models to monthly mortality data from 1980 to 2008 for pneumonia/influenza- and respiratory/circulatory-coded deaths for all ages and for those aged ⩾60 years. According to viral data, 73·5% of influenza viruses were detected between April and August in southern Brazil. There was no clear influenza season for northern Brazil. In southern Brazil, influenza-associated excess mortality was 1·4/100 000 for all ages and 9·2/100 000 person-years for persons aged ⩾60 years using underlying pneumonia/influenza-coded deaths and 10·0/100 000 for all ages and 86·6/100 000 person-years for persons aged ⩾60 years using underlying respiratory/circulatory-coded deaths. Influenza-associated excess mortality rates for southern Brazil are similar to those published for other countries. Our data support the need for continued influenza surveillance to guide vaccination campaigns to age groups most affected by this virus in Brazil.
A new species of chimaeriform (Chondrichthyes, Holocephali) from the uppermost Cretaceous of the López de Bertodano Formation, Isla Marambio (Seymour Island), Antarctica
Authors: Otero Rodrigo A. , Rubilar-Rogers David , Yury-Yañez Roberto E. , Vargas Alexander O. , Gutstein Carolina S. , Mourgues Francisco Amaro , Robert Emmanuel 
Published: October 2012

AbstractWe describe a new chimaeriform fish, Callorhinchus torresi sp. nov., from the uppermost Cretaceous (late Maastrichtian) of the López de Bertodano Formation, Isla Marambio (Seymour Island), Antarctica. The material shows it is distinct from currently known fossil and extant species of the genus, whereas the outline of the tritors (abrasive surfaces of each dental plate) shows an intermediate morphology between earlier records from the Cenomanian of New Zealand and those from the Eocene of Isla Marambio. This suggests an evolutionary trend in tritor morphology in the lineage leading to modern callorhynchids, during the Late Cretaceous-Palaeogene interval.
Long-term evaluation of endothelial function in Kawasaki disease patients
Authors: Pinto Fátima F. , Laranjo Sérgio , Paramés Filipa , Freitas Isabel , Mota-Carmo Miguel 
Published: October 2012

AbstractBackgroundKawasaki disease is an acute systemic vasculitis. Cardiac complications are frequent and include endothelial dysfunction in patients with coronary anomalies. So far, the presence of endothelial dysfunction in patients with no coronary lesions has not been demonstrated. Peripheral arterial tonometry (Endo-PAT) measures the microvascular function in response to local ischaemia and has been validated in adult population, but its use in children is scarce.AimTo evaluate endothelial dysfunction in children as a long-term complication after Kawasaki disease using Endo-PAT.MethodsWe evaluated two groups of subjects: (1) Kawasaki disease patients over 11 years of age, diagnosed for >5 years, with no coronary lesions, or any other risk factors for cardiovascular disease; (2) control group of individuals without cardiovascular risk factors. Patients and controls were clinically accessed. Endo-PAT was performed to determine reactive hyperaemia index and augmentation index.ResultsA total of 35 individuals (21 males, age 21 ± 6 years) were evaluated (group 1: 19; controls: 16). Kawasaki disease patients presented significant lower reactive hyperaemia index (1.68 ± 0.49 versus 2.31 ± 0.53; p = 0.001). Augmentation index was similar in both groups (−10 ± 7 versus −11 ± 5; p > 0.005). Most patients with Kawasaki disease disclosed endothelial dysfunction (68%) compared with only 12% in controls.ConclusionsEndo-PAT is feasible and reproducible in the child population. Endothelial dysfunction is a frequent long-term complication in patients after Kawasaki disease with normal appearing coronary arteries. However, these results need validation in a larger population.
Patient and practice factors affecting growth of infants with systemic-to-pulmonary shunt
Authors: McCrary Andrew W. , Clabby Martha L. , Mahle William T. 
Published: October 2012

AbstractBackgroundOn recognising poor growth following neonatal palliation with a systemic-to-pulmonary shunt, we sought to determine how patient- and procedure-related factors impact growth, paying attention to the role of the primary cardiologist in this process.MethodsIn a retrospective review, neonates (133 patients) receiving modified systemic-to-pulmonary artery shunts from 2002 to 2009 were studied and outpatient visits were reviewed. Patients with single- and two-ventricle circulations after shunt takedown were compared using weight-for-age z-score.ResultsSingle-ventricle patients had a higher weight-for-age z-score at neonatal surgery than two-ventricle patients (−0.4 ± 1.0 compared with −1.2 ± 0.9, with p < 0.001), but they had a greater drop in the weight-for-age z-score to the first outpatient visit (−1.1 ± 0.7 compared with −0.8 ± 0.7, with p = 0.02). After the first outpatient visit, the weight-for-age z-score was not significantly different between single-ventricle and two-ventricle patients. From multivariate analysis, a lower number of nutritional interventions by cardiologists was significantly associated with poor growth (p = 0.03). Poor growth was not associated with race, use of feeding tube, exclusive formula use, or proximity to surgical centre.ConclusionThe significant drop in the weight-for-age z-score from neonatal surgery to first outpatient visit suggests that these patients may receive inadequate nutrition. The poorest performers received the least number of outpatient changes to their diet. This finding underscores the critical role of the primary cardiologist in optimising weight gain through adjustments in nutrition.
Resource Utilization in the Emergency Department of a Tertiary Care University-Based Hospital in Tokyo Before and After the 2011 Great East Japan Earthquake and Tsunami
Authors: Shimada Mai , Tanabe Aska , Gunshin Masataka , Riffenburgh Robert H. , Tanen David A. 
Published: October 2012

AbstractIntroductionThe objective of this study was to determine the resource utilization of a tertiary care Japanese emergency department (ED) that was not immediately adjacent to the area of the 2011 Great East Japan earthquake and tsunami.MethodsA retrospective chart review was performed at a tertiary care university-based urban ED located approximately 290 km from the primary site of destruction secondary to an earthquake measuring 9.0 on the Richter Scale and the resulting tsunami. All patients who presented for a period of twelve days before and twelve days after the disaster were included. Data were collected using preformed data collection sheets, and stored in an Excel file. Abstracted data included gender, time in the ED, intravenous fluid administration, blood transfusion, oxygen, laboratories, electrocardiograms (ECGs), radiographs, ultrasound, diagnoses, surgical and medical referrals, and prescriptions written. Ten percent of the charts were reviewed for accuracy, and an error rate reported. Data were analyzed using 2-tailed t-tests, Fisher's exact tests or rank sum tests. Bonferroni correction was used to adjust P values for multiple comparisons.ResultsCharts for 1193 patients were evaluated. The error rate for the abstracted data was 3.2% (95% CI, 2.4%-4.1%). Six hundred fifty-seven patients (53% male) were evaluated in the ED after the earthquake, representing a 23% increase in patient volume. Mean patient time spent in the ED decreased from 61 minutes to 52 minutes (median decrease from 35 minutes to 32 minutes; P = .005). Laboratory utilization decreased from 51% to 43% (P = .006). The percentage of patients receiving prescriptions increased from 48% to 54% (P = .002). There was no change in the number of patients evaluated for surgical complaints, but there was an increase in the number treated for medical or psychiatric complaints.ConclusionThere was a significant increase in the number of people utilizing the ED in Tokyo after the Great East Japan earthquake and tsunami. Time spent in the ED was decreased along with laboratory utilization, possibly reflecting decreased patient acuity. This information may help in the allocation of national resources when planning for disasters.ShimadaM, TanabeA, GunshinM, RiffenburghRH, TanenDA. Resource utilization in the emergency department of a tertiary care university-based hospital in Tokyo before and after the 2011 Great East Japan Earthquake and tsunami. Prehosp Disaster Med. 2012;27(6):1-4.
Environmental innovation and policy harmonization in international oligopoly
Authors: Hattori Keisuke 
Published: October 2012

AbstractThis paper investigates firm incentives for developing environmentally clean technologies in a simple two-country model with international oligopoly and lack of regulatory commitment, and compares the incentives under price and quantity regulations with and without policy cooperation between governments. We examine whether policy coordination (choices of policy instruments or policy harmonization) encourages environmental innovation when firms have strategic innovation incentives that may influence future regulation. In a case where policies are non-cooperatively set by governments, quantity regulations yield a greater static benefit for countries; however, dynamically, price regulations encourage more innovation than quantity regulations when environmental damages are not so large. Under both price and quantity regulation regimes, cooperative policy harmonization necessarily enhances net benefits in each country, whereas it discourages firms' innovation incentives when environmental damages are not so small.
Sample Preparation Induced Artifacts in Cryo-Electron Tomographs
Authors: Plevka Pavel , Battisti Anthony J. , Winkler Dennis C. , Tars Kaspars , Holdaway Heather A. , Bator Carol M. , Rossmann Michael G. 
Published: October 2012

AbstractWe investigated the effects of sample preparation and of the exposure to an electron beam on particles in cryo-electron tomographs. Various virus particles with icosahedral symmetry were examined, allowing a comparison of symmetrically related components that should be identical in structure but might be affected differently by these imaging artifacts. Comparison of tomographic reconstructions with previously determined structures established by an independent method showed that neither freezing nor electron beam exposure produced a significant amount of shrinkage along the z axis (thickness). However, we observed damage to regions of the particles located close to the surface of the vitreous ice.