bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tandem-Repeat Patterns and Mutation Rates in Microsatellites of the Nematode Model OrganismPristionchus pacificus
Authors: Molnar Ruxandra I , Witte Hanh , Dinkelacker Iris , Villate Laure , Sommer Ralf J 
Published: November 2013

AbstractModern evolutionary biology requires integrative approaches that combine life history, population structure, ecology, and development. The nematode Pristionchus pacificus has been established as a model system in which these aspects can be studied in one organism. P. pacificus has well-developed genetic, genomic, and transgenic tools and its ecologic association with scarab beetles is well described. A recent study provided first mutation rate estimates based on mitochondrial genome sequencing and mutation accumulation line experiments that help resolve rather ancient evolutionary branches. Here, we analyzed the tandem-repeat pattern and studied spontaneous mutation rates for microsatellite markers by using the previously generated mutation accumulation lines. We found that 0.59%–3.83% of the genome is composed of short tandem repeats. We developed 41 microsatellite markers, randomly chosen throughout the genome and analyzed them in 82 mutation accumulation lines after 142 generations. A total of 31 mutations were identified in these lines. There was a strong correlation between allele size and mutation rate in P. pacificus, similar to Caenorhabditis elegans. In contrast to C. elegans, however, there is no evidence for a bias toward multistep mutations. The mutation spectrum of microsatellite loci in P. pacificus shows more insertions than deletions, indicating a tendency toward lengthening, a process that might have contributed to the increase in genome size. The mutation rates obtained for individual microsatellite markers provide guidelines for divergence time estimates that can be applied in P. pacificus next-generation sequencing approaches of wild isolates.
Genetic Architecture of Migration-Related Traits in Rainbow and Steelhead Trout,Oncorhynchus mykiss
Authors: Hecht Benjamin C , Thrower Frank P , Hale Matthew C , Miller Michael R , Nichols Krista M 
Published: November 2013

AbstractAlthough migration plays a critical role in the evolution and diversification of species, relatively little is known of the genetic architecture underlying this life history in any species. Rainbow and steelhead trout (Oncorhynchus mykiss) naturally segregate for both resident and migratory life-history types, respectively, as do other members of the salmonid family of fishes. Using an experimental cross derived from wild resident rainbow and wild migratory steelhead trout from Southeast Alaska and high throughput restriction-site associated DNA (RAD) tag sequencing, we perform a quantitative trait locus (QTL) analysis to identify the number, position, and relative contribution of genetic effects on a suite of 27 physiological and morphological traits associated with the migratory life history in this species. In total, 37 QTL are localized to 19 unique QTL positions, explaining 4–13.63% of the variation for 19 of the 27 migration-related traits measured. Two chromosomal positions, one on chromosome Omy12 and the other on Omy14 each harbor 7 QTL for migration-related traits, suggesting that these regions could harbor master genetic controls for the migratory life-history tactic in this species. Another QTL region on Omy5 has been implicated in several studies of adaptive life histories within this species and could represent another important locus underlying the migratory life history. We also evaluate whether loci identified in this out-crossed QTL study colocalize to genomic positions previously identified for associations with migration-related traits in a doubled haploid mapping family.
oPOSSUM-3: Advanced Analysis of Regulatory Motif Over-Representation Across Genes or ChIP-Seq Datasets
Authors: Kwon Andrew T , Arenillas David J , Hunt Rebecca Worsley , Wasserman Wyeth W 
Published: November 2013

AbstractoPOSSUM-3 is a web-accessible software system for identification of over-represented transcription factor binding sites (TFBS) and TFBS families in either DNA sequences of co-expressed genes or sequences generated from high-throughput methods, such as ChIP-Seq. Validation of the system with known sets of co-regulated genes and published ChIP-Seq data demonstrates the capacity for oPOSSUM-3 to identify mediating transcription factors (TF) for co-regulated genes or co-recovered sequences. oPOSSUM-3 is available at http://opossum.cisreg.ca.
The Lsktm1 Locus Modulates Lung and Skin Tumorigenesis in the Mouse
Authors: Galvan Antonella , Colombo Francesca , Noci Sara , Pazzaglia Simonetta , Mancuso Mariateresa , Manenti Giacomo , Broman Karl W , Saran Anna , Dragani Tommaso A 
Published: November 2013

Abstract
Alleles derived from skin tumor−resistant Car-R mice provide resistance to both skin and lung tumorigenesis over the susceptibility of the SWR/J strain. In an effort to map tumor modifier loci affecting both tumor types, we carried out a genetic linkage analysis in backcross SWR/J x (SWR/J x Car-R) mice and identified a locus (Lsktm1) on chromosome 1 linked to both skin (LOD score = 3.93) and lung (LOD score = 8.74) tumorigenesis. Two genes, Igfbp5 and Igfbp2, residing in this locus and belonging to the insulin-like growth factor binding protein family were expressed at significantly greater levels in normal lung tissue from cancer-resistant Car-R mice than in cancer-susceptible SWR/J mice. Overexpression of the recombinant Igfbp5 and Igfbp2 genes in two lung cancer cell lines significantly inhibited clonogenicity (P &lt; 0.0001). Collectively, we have identified a single polymorphic locus that affects skin and lung tumorigenesis and identify Igfbp5 and Igfbp2 as candidate modifier genes of lung tumorigenesis.
Otitis Media with Effusion: Experiences of Children with Cleft Palate and Their Parents
Authors: Tierney Stephanie , O'brien Kevin , Harman Nicola L. , Sharma Ravi K. , Madden Colm , Callery Peter 
Published: November 2013

Objective To explore the views of children with cleft palate and their parents about daily life with otitis media with effusion and associated hearing loss. Design A qualitative study. Semistructured interviews were used to collect data from parents. Participatory techniques, including activities on a tablet computer, were used to collect data from children. Framework analysis was applied to interview transcripts. Setting Two English cleft units. Participants A purposive sample of parents of 37 children aged 0 to 11 years with experience of otitis media with effusion. Their children also took part if aged 6 to 11 years (n = 22). Results Themes related to the following: (1) emotions (frustration, anger, sadness, happiness, anxiety), (2) educational experiences (struggling at school, having to sit at the front of the class, requiring extra support, missing lessons for appointments or due to ear infections), (3) social interactions (isolation, communication, reliance on siblings, participation in activities). Conclusions A number of areas of interviewees' everyday life were affected by the presence of otitis media with effusion. Parents may need to be forewarned about the possible ongoing nature of this condition and its impact on a child's social and emotional experiences. Children may also benefit from age-appropriate information about otitis media with effusion and its treatment, including information on hearing tests, to help reduce any anxiety.
Pregnancy Outcomes According to Dialysis Commencing Before or After Conception in Women with ESRD
Authors: Jesudason Shilpanjali , Grace Blair S. , McDonald Stephen P. 
Published: November 2013

Summary

Background and objectives
Pregnancy in ESRD is rare and poses substantial risk for mother and baby. This study describes a large series of pregnancies in women undergoing long-term dialysis treatment and reviews maternal and fetal outcomes. Specifically, women who had conceived before and after starting long-term dialysis are compared.

Design, setting, participants, & measurement
All pregnancies reported to the Australian and New Zealand Dialysis and Transplantation Registry from 2001 to 2011 (n=77), following the introduction of specific parenthood data collection, were analyzed.

Results
Between 2001 and 2011, there were 77 pregnancies among 73 women. Of these, 53 pregnancies were in women who conceived after long-term dialysis was established and 24 pregnancies occurred before dialysis began. The overall live birth rate (after exclusion of elective terminations) was 73%. In pregnancies reaching 20 weeks gestation, the live birth rate was 82%. Women who conceived before dialysis commenced had significantly higher live birth rates (91% versus 63%; P=0.03), but infants had similar birthweight and gestational age. This difference in live birth rate was primarily due to higher rates of early pregnancy loss before 20 weeks in women who conceived after dialysis was established. In pregnancies that reached 20 weeks or more, the live birth rate was higher in women with conception before dialysis commenced (91% versus 76%; P=0.28). Overall, the median gestational age was 33.8 weeks (interquartile range, 30.6–37.6 weeks) and median birthweight was 1750 g (interquartile range, 1130–2417 g). More than 40% of pregnancies reached >34 weeks’ gestation; prematurity at <28 weeks was 11.4% and 28-day neonatal survival rate was 98%.

Conclusions
Women with kidney disease who start long-term dialysis after conception have superior live birth rates compared with those already established on dialysis at the time of conception, although these pregnancies remain high risk.
Evaluation and Evolution of Diabetes Mobile Applications: Key Factors for Health Care Professionals Seeking to Guide Patients
Authors: Ristau Ryan A. , Yang Jessica , White John R. 
Published: November 2013

In Brief
Diabetes management has evolved with the presence of smartphones, offering a plethora of applications, or “apps,” to assist technologically savvy users. This rapidly growing field of mobile apps has hosted a multifarious selection ranging from novice startup software to programs designed for professional use. This article reviews some crucial factors for consideration when seeking to optimize diabetes management via smartphone.
Power and Pitfalls of Social Media in Diabetes Care
Authors: McMahon Kevin L. 
Published: November 2013

In Brief
This article addresses modern patients' insatiable need for information as they make daily choices in managing diabetes. It introduces the resources available to diabetes patients and caregivers against the backdrop of the limitations medical practices face in meeting patients' information and support needs. Social media are described, along with ways in which health care providers might leverage digital technology to better support their patients. The author describes trends and studies that suggest how social media might be used in the future not only to meet patients' needs, but also to streamline clinical workflow.
Reinforcement of Teeth With Simulated Coronal Fracture and Immature Weakened Roots Using Resin Composite Cured by a Modified Layering Technique
Authors: Seyam RS , Mobarak EH 
Published: November 2013

SUMMARY

Objective
The purpose of this study was to evaluate the strengthening effect of resin composite, cured by a modified layering protocol, for teeth with simulated coronal fracture and weakened immature roots.

Methods
Fifty maxillary teeth were decoronated and their apices sectioned to standardize the length to 12 mm. Prepared teeth were equally distributed into five groups. Group 1VF root apices were flared with Pesso drills up to size 6. The roots were flared until a dentin thickness of only 1 ± 0.2 mm remained. Root ends were filled with mineral trioxide aggregate. The canals were backfilled with Vertise Flow following a modified layering protocol using two light-transmitting posts size 6 and 3. Next, a DT light post size 2 was cemented using the same material. Groups 2TS/MF and 3ED/PF were prepared and cured in the same way as group 1VF but filled with Clearfil Tri-S Bond/Majesty Flow and ED Primer II/Panavia F2.0 respectively. Group 4UF was similarly prepared but left unfilled (control). In group 5NW, roots were unflared but similarly filled as in group 3ED/PF. After 24 hours of storage, the fracture load was measured. The degree of cure for each tested material was indirectly measured using microhardness at different root levels (cervical, middle, and apical). Data were analyzed using one-way analysis of variance followed by Newman-Keuls post hoc test.

Results
Fracture load results revealed that groups 1VF and 2TS/MF had no statistically significant difference from group 5NW (p&gt;0.05). For each tested material, no significant difference was found among microhardness values at different root levels.

Conclusion
It may be possible to reinforce the teeth with coronal fracture and immature weakened roots to be comparable with unweakened ones when composite is applied and cured by the modified layering technique.
Effect of the Prevalent Insect Species of Yam Chips on Consumers’ Acceptability of Yam Paste
Authors: Babarinde Samuel Adelani , Babarinde Grace Oluwakemi , Odewole Adeola Foluso , Alagbe Olubukola Omotoyosi 
Published: November 2013

Abstract
A survey was carried out between March and August 2010 to identify insect pests of stored yam chips in Ogbomoso Metropolis, Nigeria. Dried yam chips were randomly obtained from old stocks in 6 local markets within the metropolis on monthly basis. There was a significant (P < 0.05) difference in insect abundance based on month of sampling and insect species. Dinoderus porcellus (Coleoptera: Bostrychidae) was the most abundant species. Other species encountered were Araecerus fasciculatus (Degeer) (Coleoptera: Anthribidae), Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) and Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). When chips were infested with Dinoderus porcellus and stored for 3 months, reconstituted thick paste (amala) was made from the flour and was evaluated for consumers’ acceptability. Sensory characteristics such as odour, colour, texture, appearance, taste and overall acceptability were significantly (P < 0.05) affected when compared with the paste prepared from the uninfested chips.
An Analysis of the Creative Potential in Individual Regions of The Czech Republic
Authors: Kloudová Jitka , Chwaszcz Ondřej 
Published: November 2013

Abstract
Purpose of the article: Although the economic growth and society are two independent terms at the first sight, they are in fact closely connected and interact with each other. The main topic of this work is the creative economy, which is considered to be a part of growth theories. Thanks to the new approach, theorists supplement these theories with the demographic and the socio-cultural factor. First, the work establishes a comprehensive theoretical framework for economic growth (linked with creativity). Furthermore, it analyses the representation of creativity in the regions of the Czech Republic.
Methodology/methods: The primary aim of this work is to carry out an analysis that charts the representation of the creative potential in the regions of the Czech Republic. The analysis uses the new creative index, which was created by modifying of the Richard Florida´s creative index. Consequently, the work uses the new creative index within the framework of smaller regional units as opposed to national groupings. Additional advantages of this model include the number and measurability of the indexes.
Scientific aim: The aim of this work is not just demonstrating the dependence between the presence of creative people and economic growth, but in addition, the work aims to seek out the factors that influence the allocation of creative employees.
Findings: The work demonstrates high mutual dependence between creativity and economic growth with the help of the correlation coefficient. The assumptions that creative regions are more attractive on the social and knowledge basis were also confirmed.
Conclusions (limits,implications etc): Territorial units must consequently work to develop conditions, which support the establishment and the development of the creative environment in order to retain their competitiveness and support the economic growth.
Knowledge Management and Organizational Culture: An Exploratory Study
Authors: Li Bing , Zhang Jianpeng , Zhang Xiaoxia 
Published: November 2013

Abstract
Purpose of the article Knowledge has been considered as the strategic assets and become the source of competitive advantage in organizations. Knowledge management thus receives the extraordinary attention from the top management. Many organizational factors have influences on knowledge management practices. This paper attempts to explore the empirical relationship between knowledge management and organizational culture in the specific situation of China’s commercial banking industry. Methodology/methods The relationship between knowledge management and organizational culture is quantitatively investigated by surveying bank managers. The scale of SECI modes is used to measure knowledge management process and the scale of Denison Organizational Culture Survey (DOCS) is used to measure organizational culture. We explore the underlying relationship by employing the statistical analyses such as correlation, regression and structural equation modeling. Scientific aim The research aims at testing the relationship between knowledge management and organizational culture, and furthermore if there exist linkages between cultural traits and SECI modes. Findings The results of the empirical study confirm the great and positive effect that organizational culture has on knowledge management. Different cultural traits contribute to different SECI modes. Conclusions For obtaining successful knowledge management practices in organizations, it is better to concern about the relationship between knowledge management and organizational culture. The limitation in the paper is the sampling size, which will be solved by an industry-wide survey in our future research.
Validity of the AM-PAC “6-Clicks” Inpatient Daily Activity and Basic Mobility Short Forms
Authors: Jette Diane U. , Stilphen Mary , Ranganathan Vinoth K. , Passek Sandra D. , Frost Frederick S. , Jette Alan M. 
Published: November 2013

Background
Standardized assessment of patients' activity limitations in acute care settings can provide valuable information. Existing measures have not been widely implemented.

Objectives
The aim of this study was to provide evidence for validity of scores on Activity Measure for Post-Acute Care (AM-PAC) “6-Clicks” measures of basic mobility and daily activity in acute care.

Design
A retrospective measurement study was conducted.

Methods
The study used a database from one health system containing “6-Clicks” scores from first and last physical therapist and occupational therapist visits for 84,446 patients. Validity was analyzed by examining differences in “6-Clicks” scores across categories of patient characteristics; the ability of “6-Clicks” scores to predict patients' having more than one therapy visit; correlation of “6-Clicks” scores with Functional Independence Measure (FIM) scores; and internal responsiveness over the episode of care. Internal consistency reliability also was determined.

Results
The “6-Clicks” scores differed across patients' age, preadmission living situation, and number of therapy visits. The areas under receiver operating characteristic curves derived using “6-Clicks” scores at the first visit to predict patients receiving more than one visit were 0.703 and 0.652 using basic mobility and daily activity scores, respectively. The “6-Clicks” scores at the final visit were correlated with scores on subscales of the FIM completed on admission to inpatient rehabilitation facilities (r=.65 and .69). Standardized response means were 1.06 and 0.95 and minimal detectable changes with 90% confidence level (MDC90) were 4.72 and 5.49 for basic mobility and daily activity scores, respectively. Internal consistency reliability of basic mobility and daily activity scores was .96 and .91, respectively.

Limitations
Using clinical databases for research purposes has limitations, including missing data, misclassifications, and selection bias. Rater reliability is not known.

Conclusions
This study provides evidence for the validity of “6-Clicks” scores for assessing patients' activity limitations in acute care settings.
Surgery for insular low-grade glioma: predictors of postoperative seizure outcome
Authors: Ius Tamara , Pauletto Giada , Isola Miriam , Gregoraci Giorgia , Budai Riccardo , Lettieri Christian , Eleopra Roberto , Fadiga Luciano , Skrap Miran 
Published: November 2013

Object
Although a number of recent studies on the surgical treatment of insular low-grade glioma (LGG) have demonstrated that aggressive resection leads to increased overall patient survival and decreased malignant progression, less attention has been given to the results with respect to tumor-related epilepsy. The aim of this investigation was to evaluate the impact of volumetric, histological, and intraoperative neurophysiological factors on seizure outcome in patients with insular LGG.

Methods
The authors evaluated predictors of seizure outcome with special emphasis on both the extent of tumor resection (EOR) and the tumor's infiltrative pattern quantified by computing the difference between the preoperative T2- and T1-weighted MR images (ΔVT2T1) in 52 patients with preoperative drug-resistant epilepsy.

Results
The 12-month postoperative seizure outcome (Engel class) was as follows: seizure free (Class I), 67.31%; rare seizures (Class II), 7.69%; meaningful seizure improvement (Class III), 15.38%; and no improvement or worsening (Class IV), 9.62%. Poor seizure control was more common in patients with a longer preoperative seizure history (p < 0.002) and higher frequency of seizures (p = 0.008). Better seizure control was achieved in cases with EOR ≥ 90% (p < 0.001) and ΔVT2T1 < 30 cm3 (p < 0.001). In the final model, ΔVT2T1 proved to be the strongest independent predictor of seizure outcome in insular LGG patients (p < 0.0001).

Conclusions
No or little postoperative seizure improvement occurs mainly in cases with a prevalent infiltrative tumor growth pattern, expressed by high ΔVT2T1 values, which consequently reflects a smaller EOR.
La participation des pères aux soins et à l’éducation des enfants [L’influence des rapports sociaux de sexe entre les parents et entre les générations]
Authors: Brugeilles Carole , Sebille Pascal 
Published: November 2013

The participation of fathers in caring for and raising children. The influence of the social relations of gender between parents and between generations.
Demographic and legislative developments, along with changes in attitude and behaviour, since the 1970’ s have helped bring about a new image of the father who is more involved in his relation with his children and in assuming parental activities. However, the ideal of an equal division is not substantiated in reality. After highlighting the division of parental tasks in couples, the research attempts to explain paternal under-investment by focusing both on social relations of gender in effect in the parents’ generation and on those defining relations between parent-child generations. The analysis based on the Gender and generation survey (GGS) by the Institut d’études démographiques (INED France) demonstrates the inequality in paternal investment by type of task (dressing, putting to bed, accompanying journeys, leisure and homework) and the underlying factors at stake in social relations of gender.