bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selection of Yeasts as Starter Cultures for Table Olives
Authors: Bevilacqua Antonio , Beneduce Luciano , Sinigaglia Milena , Corbo Maria Rosaria 
Published: April 2013

Abstract Ninety‐nine yeasts were isolated from Bella di Cerignola table olives; first, the strains were studied in relation to their ability to produce biogenic amines in a laboratory medium and 49 strains were positive to this assay and cut off from the research. The remaining 50 strains were characterized for their enzymatic traits (β‐glucosidase, catalase, pectolytic, xylanolytic, and lipolytic activities) and for their ability to grow at different temperatures, pHs, with salt or lactic/acetic acids added. Data were used for the evaluation of growth index and submitted to cluster and principal component analyses to choose the most promising 4 strains. In the final step of the research, the strains were inoculated as a cocktail in a model brine, containing different amounts of salt (4% to 12%) and glucose (0% to 3%), and adjusted to different pHs (4.0 to 9.0). Data analysis through a multiple regression procedure highlighted that salt, glucose, and pH acted in a different way within the storage and NaCl affected yeast growth only for few days, and then glucose and pH played a major role.Practical Application Olive fermentation relies upon a complex microflora, including lactic acid bacteria and yeasts; the selection of suitable strains of yeasts intended as starter cultures, as well as their inoculation in brines, could improve the fermentation.
Increased hepcidin expression in non‐small cell lung cancer tissue and serum is associated with clinical stage
Authors: Chen Qian , Wang Li , Ma Yuchao , Wu Xianning , Jin Longyu , Yu Fenglei 
Published: April 2013

AbstractBackgroundHepcidin is a small secreted peptide that plays a key role in iron metabolism. A high level of hepcidin expression may be implicated in colorectal cancer; however, the relationship between hepcidin and lung cancer has not yet been studied.MethodsSerum hepcidin‐25, bone morphogenetic protein (BMP)‐2, and interleukin (IL)‐6 concentration in 53 patients and 16 non‐cancerous individuals was measured by enzyme‐linked immune sorbent assay. Reverse transcriptase quantitative polymerase chain reaction (RT‐qPCR) was utilized to study the expression of hepcidin mRNA in paired tumor and non‐tumor lung tissues in surgical specimens from 65 patients with non small cell lung cancer (NSCLC), as well as in six types of lung cancer cell lines and human bronchial epithelial (HBE) cells. Hepcidin protein expression and cellular localization in NSCLC was determined by immunohistochemistry.ResultsThe serum hepcidin‐25 concentration was higher in patients with NSCLC than in non‐cancerous individuals, and was positively correlated with serum BMP2 concentration, but negatively with serum IL‐6 levels. Serum hepcidin was also correlated with lymph node metastasis and clinical stage. Hepcidin mRNA expression was higher in cancerous tissues of NSCLC than in normal pulmonary tissues (P = 0.001). Hepcidin mRNA levels in four lung carcinoma cell lines were higher than in HBE cells. Immunohistochemistry showed that hepcidin protein was increased in cancerous tissues of NSCLC.ConclusionsThe level of hepcidin expression increased in NSCLC tissue and serum. Serum hepcidin‐25 level was associated with lymph node metastasis and tumor clinical stage in patients with NSCLC.
Estimating the effect of temporally autocorrelated environments on the demography of density‐independent age‐structured populations
Authors: Engen Steinar , Sæther Bernt‐Erik , Armitage Kenneth B. , Blumstein Daniel T. , Clutton‐Brock Tim H. , Dobson F. Stephen , Festa‐Bianchet Marco , Oli Madan K. , Ozgul Arpat 
Published: April 2013

Summary

In age‐structured populations, environmental autocorrelations influence the long‐run population growth rate as well as the variance in future population size. We used the concept of individual reproductive value to examine how autocorrelated environments affect the dynamics of age‐structured populations, leading to transparent interpretations and estimation of these effects.

Environmental autocorrelation is expressed by the covariances between mean individual reproductive values for each age class and size of the same age class with stochastic components depending only on noise matrices from previous years. Thus, if an age class that is large in a given year also tends to perform better than the temporal average of that class in the contribution per individual to future population sizes, then the environmental autocorrelation will be positive.

We use a simple model with temporal autocorrelation in recruitment rate to illustrate the theory through analytical results as well as stochastic simulations. We show how the effect of environmental autocorrelation, the term included in the long‐run growth rate, as well as influencing the variance of future population size, can be estimated using a combination of individual‐based demographic data and time series of fluctuations in age composition without estimating autocorrelations and cross‐correlations of large numbers of age‐specific vital rates.

The method was applied to data from four mammal species. These analyses revealed that the influence of autocorrelations in the environmental noise on the dynamics of these species was small and in two populations almost negligible.

The theoretical explorations as well as the empirical estimates indicate that the temporal scaling of the environmental autocorrelation must be long to substantially affect the long‐term population growth rate. The white noise approximation is therefore often very accurate.
TEN‐like eruption in setting of EBV positive T‐cell lymphoproliferative disease with HLH, in a child
Authors: Sharma Niyati , Clark Jonathan , Pham Hiep , Efron Daryl , MacGregor Duncan , O'Keefe Rodney , Orchard David 
Published: April 2013

AbstractHaemophagocytic lymphohistiocytosis complicating Epstein–Barr virus positive T‐cell lymphoproliferative disease of childhood is a rare and life‐threatening entity. We report a child with this condition presenting with a toxic epidermal necrolysis‐like eruption.
Skin recruitment of monomyeloid precursors involves human herpesvirus‐6 reactivation in drug allergy
Authors: Hashizume H. , Fujiyama T. , Kanebayashi J. , Kito Y. , Hata M. , Yagi H. 
Published: April 2013

AbstractBackgroundIn drug‐induced hypersensitivity syndrome (DIHS), latent human herpesvirus (HHV)‐6 is frequently reactivated in association with flaring of symptoms such as fever and hepatitis. We recently demonstrated an emergence of monomyeloid precursors expressing HHV‐6 antigen in the circulation during this clinical course.MethodsTo clarify the mechanism of HHV‐6 reactivation, we immunologically investigated peripheral blood mononuclear cells (PBMCs), skin‐infiltrating cells, and lymphocytes expanded from skin lesions of patients with DIHS.ResultsThe circulating monomyeloid precursors in the patients with DIHS were mostly CD11b+CD13+CD14−CD16high and showed substantial expression of skin‐associated molecules, such as CCR4. CD13+CD14− cells were also found in the DIHS skin lesions, suggesting skin recruitment of this cell population. We detected high levels of high‐mobility group box (HMGB)‐1 in blood and skin lesions in the active phase of patients with DIHS and showed that recombinant HMGB‐1 had functional chemoattractant activity for monocytes/monomyeloid precursors in vitro. HHV‐6 infection of the skin‐resident CD4+ T cells was confirmed by the presence of its genome and antigen. This infection was likely to be mediated by monomyeloid precursors recruited to the skin, because normal CD4+ T cells gained HHV‐6 antigen after in vitro coculture with highly virus‐loaded monomyeloid precursors from the patients.ConclusionsOur results suggest that monomyeloid precursors harboring HHV‐6 are navigated by HMGB‐1 released from damaged skin and probably cause HHV‐6 transmission to skin‐infiltrating CD4+ T cells, which is an indispensable event for HHV‐6 replication. These findings implicate the skin as a cryptic and primary site for initiating HHV‐6 reactivation.
IgE‐mediated basophil tumour necrosis factor alpha induces matrix metalloproteinase‐9 from monocytes
Authors: Falkencrone S. , Poulsen L. K. , Bindslev‐Jensen C. , Woetmann A. , Odum N. , Poulsen B. C. , Blom L. , Jensen B. M. , Gibbs B. F. , Yasinska I. M. , Sumbayev V. V. , Skov P. S. 
Published: April 2013

AbstractBackgroundIgE‐mediated activation of mast cells has been reported to induce the release of tumour necrosis alpha (TNF‐α), which may display autocrine effects on these cells by inducing the generation of the tissue remodelling protease matrix metalloproteinase‐9 (MMP‐9). While mast cells and basophils have been shown to express complementary and partially overlapping roles, it is not clear whether a similar IgE/TNF‐α/MMP‐9 axis exists in the human basophil. The purpose of this study was thus to investigate whether IgE‐mediated activation of human basophils induces TNF‐α and MMP‐9 release.MethodsHuman peripheral blood mononuclear cells (PBMC), isolated basophils and monocytes were stimulated up to 21 h with anti‐IgE. Mediator releases were assessed by ELISA, and surface expressions of mediators were detected by flow cytometry. Upregulation of cytokine production was detected by Western blot and polymerase chain reaction (PCR).ResultsIgE‐mediated activation of basophils induced the synthesis and release of both TNF‐α and MMP‐9 from PBMC. In contrast, IgE‐mediated activation of purified basophils induced the release and cellular expression of TNF‐α but not MMP‐9. Isolated monocytes did not release MMP‐9 upon anti‐IgE stimulation, but MMP‐9 release was induced by stimulating monocytes with supernatants from activated basophils, and this release was inhibited by anti‐TNF‐α neutralizing antibodies.ConclusionOur results strongly indicate that human basophils release TNF‐α following IgE‐dependent activation and that this cytokine subsequently stimulates MMP‐9 release from monocytes. These findings support a direct involvement of basophils in inflammation as well as suggesting a role for the basophil in tissue remodelling.
Intra‐abdominal hypertension in the current era of modern trauma resuscitation
Authors: Mahmood Ismail , Mahmood Saeed , Parchani Ashok , Kumar Suresh , El‐Menyar Ayman , Zarour Ahmad , Al‐Thani Hassan , Latifi Rifat 
Published: April 2013

AbstractBackground This study aimed to determine the incidence and outcome of post‐traumatic (PT) intra‐abdominal hypertension (IAH) and abdominal compartment syndrome (ACS) after the advances in haemostatic resuscitation.MethodsThis is a prospective cohort study from January 2009–December 2011 involving patients with PT haemorrhagic shock. Patients' demographics, fluid resuscitation (<24 h) and damage control laparotomy (DCL), morbidity and mortality were assessed. Patients were divided into group 1 (no DCL) and group 2 (DCL needed). Further, group 1 was subdivided into three subgroups (IA pressure (IAP) <12, 12–20 and >20 mmHg).Results One hundred seventeen patients enrolled in the study (102 in group 1 and 15 in group 2) with a mean age of 35 ± 14, injury severity score (ISS) of 23 ± 10, base deficit of −8.7 ± 2.7 mmol/L, serum lactate of 4.6 ± 2.5 mg/dL and haemoglobin level of 8.8 ± 2. Patients received 7 ± 5 red blood cell units, 6 ± 4.7 fresh frozen plasma units and 8.3 ± 3 L of crystalloid per 24 h. There were significant difference between the two groups regarding crystalloid volume, blood transfusion, base deficit and intensive care unit length of stay. However, mortality was higher in group 2 (20% versus 6%). IAP ≥ 20 mmHg was reported in 16.7% patients, while 25.5% had IAP < 12 and 57.8% had IAP of 12–20 mmHg. Patients with IAP > 20 had worse metabolic acidosis and received more blood compared with other groups. One patient died because of ACS (0.9%). Overall multiorgan failure and mortality were 5 and 7.7%, respectively.ConclusionWith current practice of minimal fluid resuscitation and liberal use of damage control strategies among trauma patients, the IAH was common transient phenomena but the incidence of ACS is remarkably low.
Relationship between macular pigment and visual acuity in eyes with early age‐related macular degeneration
Authors: Puell María C. , Palomo‐Alvarez Catalina , Barrio Ana R. , Gómez‐Sanz Fernando J. , Pérez‐Carrasco María Jesús 
Published: April 2013

Abstract.Purpose:  Today the extent to which MP impacts visual function in early AMD remains unclear. This study examines the relationship between macular pigment optical density (MPOD) and high‐contrast visual acuity (HC‐VA) and low‐contrast visual acuity (LC‐VA) in eyes with early age‐related macular degeneration (AMD).Methods:  Measurements were made in 22 subjects with early AMD and 27 healthy control subjects. Distance best‐corrected VA was measured using HC (96%) and LC (10%) Bailey‐Lovie logMAR letter charts under photopic luminance conditions. MPOD was determined at the fovea through apparent motion photometry using the cathode ray tube‐based Metropsis psychophysical vision test (Cambridge Research Systems).Results:  No significant differences in foveal MPOD were detected between the control eyes (0.30 ± 0.24 log units) and eyes with early AMD (0.27 ± 0.15 log units). Neither were differences detected between the two groups in mean HC‐ and LC‐VA. Foveal MPOD showed significant correlation with both photopic HC‐VA (r = −0.47, p = 0.0008) and LC‐VA (r = −0.46, p = 0.0008) such that as MPOD increased, photopic HC‐VA and LC‐VA improved (lower logMAR values).Conclusions:  Low MP levels were related to worse visual function in both healthy eyes and eyes with early AMD. Our findings provide direction for future studies designed to improve retinal function through the use of oral supplements known to increase MP levels, especially in eyes with AMD and a low MPOD.
Differential use of learning strategies in first‐year higher education: The impact of personality, academic motivation, and teaching strategies
Authors: Donche Vincent , De Maeyer Sven , Coertjens Liesje , Van Daal Tine , Van Petegem Peter 
Published: April 2013

BackgroundAlthough the evidence in support of the variability of students' learning strategies has expanded in recent years, less is known about the explanatory base of these individual differences in terms of the joint influences of personal and contextual characteristics.AimsPrevious studies have often investigated how student learning is associated with either personal or contextual factors. This study takes an integrative research perspective into account and examines the joint effects of personality, academic motivation, and teaching strategies on students' learning strategies in a same educational context in first‐year higher education.SampleIn this study, 1,126 undergraduate students and 90 lecturers from eight professional bachelor programmes in a university college participated.MethodsSelf‐report measures were used to measure students' personality, academic motivation, and learning strategies. Students' processing and regulation strategies are mapped using the Inventory of Learning Styles. Key characteristics of more content‐focused versus learning‐focused teaching strategies were measured. Multivariate multi‐level analysis was used to take the nested data structure and interrelatedness of learning strategies into account.ResultsDifferent personality traits (openness, conscientiousness, and neuroticism) and academic motivation (amotivation, autonomous, and controlled motivation) were found to be independently associated with student learning strategies. Besides these student characteristics, also teaching strategies were found to be directly associated with learning strategies.ConclusionsThe study makes clear that the impact of teaching strategies on learning strategies in first‐year higher education cannot be overlooked nor overinterpreted, due to the importance of students' personality and academic motivation which also partly explain why students learn the way they do.
Campbell, Robert et al ed (2012) Academic and professional publishingChandos Publishing (Cambridge) isbn 978‐1‐84334‐669‐2 510 pp £60 (paper and e‐versions) www.woodheadpublishing.com/97818433346692
Authors: Hartley James 
Published: April 2013

SummaryThis book contains twenty chapters on academic and professional publishing. Some of the content areas are concerned with academic writing—such as those on plagiarism and peer review. Others discuss the effects of new technology on publishing texts and journals, while yet others focus more specifically on publishing practices, business models, sales and finances. If any of those aspects of this book is likely to be relevant to you, I advise that you will probably find only parts of it of interest or use.James Hartley
Honigsfeld, Andrea & Dove, Maria G ed (2012) Co‐teaching and other collaborative practices in the efl/esl classroomIAP (Charlotte, NC) isbn 978‐1‐61735‐686‐5 283 pp $39.09 http://www.infoagepub.com/products/Co‐Teaching‐Collaborative‐Practices‐EFL‐ESL‐Classroom
Authors: Laborda Jesús García 
Published: April 2013

SummaryThis welcome volume runs from theory to practice by presenting aspects of design, planning and field work in a topic which has had limited attention so far. Teachers who work in bilingual or inclusive courses will find it challenging but able to help them to understand and share experiences with people teaching in those and other bicultural settings. If the content of this book is likely to be relevant to you, I recommend you borrow a copy for a while.Jesús García Laborda
A phase II study of Givinostat in combination with hydroxycarbamide in patients with polycythaemia vera unresponsive to hydroxycarbamide monotherapy
Authors: Finazzi Guido , Vannucchi Alessandro M. , Martinelli Vincenzo , Ruggeri Marco , Nobile Francesco , Specchia Giorgina , Pogliani Enrico Maria , Olimpieri Odoardo Maria , Fioritoni Giuseppe , Musolino Caterina , Cilloni Daniela , Sivera Piera , Barosi Giovanni , Finazzi Maria Chiara , Tollo Silvia Di , Demuth Tim , Barbui Tiziano , Rambaldi Alessandro 
Published: April 2013

SummaryGivinostat, a histone‐deacetylase inhibitor (HDACi), inhibits proliferation of cells bearing the JAK2 V617F mutation and has shown significant activity with good tolerability in patients with chronic myeloproliferative neoplasms (MPN). In this multicentre, open‐label, phase II study, 44 patients with polycythaemia vera (PV), unresponsive to the maximum tolerated doses (MTD) of hydroxycarbamide (HC), were treated with Givinostat (50 or 100 mg/d) in combination with MTD of HC. The European LeukaemiaNet response criteria were used to assess the primary endpoint after 12 weeks of treatment. Complete or partial response was observed in 55% and 50% of patients receiving 50 or 100 mg of Givinostat, respectively. Control of pruritus was observed in 64% and 67% of patients in the 50 and 100 mg groups, respectively. The combination of Givinostat and HC was well tolerated: eight patients (18%) discontinued, four in each treatment arm; grade 3 adverse events were reported in one patient (4·5%) in each treatment arm. The combined use of Givinostat and HC was safe and clinically effective in HC‐unresponsive PV patients.
Mesenchymal stem cells (MSCs) from scleroderma patients (SSc) preserve their immunomodulatory properties although senescent and normally induce T regulatory cells (Tregs) with a functional phenotype: implications for cellular-based therapy
Authors: Cipriani P , Di Benedetto P , Liakouli V , Del Papa B , Di Padova M , Di Ianni M , Marrelli A , Alesse E , Giacomelli R 
Published: April 2013

Summary
Systemic sclerosis (SSc) is a chronic disease, with early activation of the immune system. The aim of our work was to address how SSc–mesenchymal stem cells (MSCs), although senescent, might preserve specific immunomodulatory abilities during SSc. MSCs were obtained from 10 SSc patients and 10 healthy controls (HC). Senescence was evaluated by assessing cell cycle, β-galactosidase (β-Gal) activity, p21 and p53 expression; doxorubicin was used as acute senescence stimulus to evaluate their ability to react in stressed conditions. Immunomodulatory abilities were studied co-culturing MSCs with peripheral blood mononuclear cells (PBMCs) and CD4+ cells, in order to establish both their ability to block proliferation in mixed lymphocyte reaction and in regulatory T cells (Tregs) induction. SSc–MSC showed an increase of senescence biomarkers. Eighty per cent of MSCs were in G0–G1 phase, without significant differences between SSc and HC. SSc–MSCs showed an increased positive β-Gal staining and higher p21 transcript level compared to HC cells. After doxorubicin, β-Gal staining increased significantly in SSc–MSCs. On the contrary, doxorubicin abolished p21 activation and elicited p53 induction both in SSc– and HC–MSCs. Interleukin (IL)-6 and transforming growth factor (TGF)-β-related transcripts and their protein levels were significantly higher in SSc–MSCs. The latter maintained their immunosuppressive effect on lymphocyte proliferation and induced a functionally regulatory phenotype on T cells, increasing surface expression of CD69 and restoring the regulatory function which is impaired in SSc. Increased activation of the IL-6 pathway observed in our cells might represent an adaptive mechanism to senescence, but preserving some specific cellular functions, including immunosuppression.
Vascular Endothelial Growth Factor Gene Promoter Polymorphisms and Alzheimer's Disease Risk: A Meta‐Analysis
Authors: Liu Sheng‐Yuan , Zeng Fang‐Fang , Chen Zhong‐Wei , Wang Chang‐Yi , Zhao Bin , Li Ke‐Shen 
Published: April 2013

SummaryAimConclusions on the association between polymorphisms in the vascular endothelial growth factor (VEGF) gene promoter and risk of Alzheimer's disease (AD) are ambiguous, and sufficient evaluation of the association is lacking. Therefore, we performed a meta‐analysis of observational studies to explore the association between polymorphisms in the VEGF gene promoter and AD risk.MethodsBibliographical searches were performed in the MEDLINE, EMBASE, and China National Knowledge Infrastructure (CNKI) databases without any language limitations. Three investigators independently assessed abstracts for relevant studies and independently reviewed all eligible studies. A meta‐analysis was conducted using a fixed‐ or random‐effects model. Odds ratios (ORs) and their 95% confidence intervals (CIs) were used to assess the strength of association. All statistical analyses were performed using Stata 11.0 software.ResultsThe meta‐analysis of 2787 AD cases and 2841 controls from eight published case‐control studies on the ‐2578C/A polymorphism and 1422 AD cases and 1063 controls from four studies on the ‐1154G/A polymorphism did not show any significant associations. However, in a subgroup analysis stratified by the presence of APOE є4, associations were observed with APOE ε4 (‐) for ‐2578C/A (A vs. C: OR = 1.22, 95% CI = 1.04–1.43, P = 0.014; A/A vs. C/C: OR = 1.59, 95% CI = 1.11–2.27, P = 0.011 and A/A vs. A/C + C/C: OR = 1.46, 95% CI = 1.08–1.99, P = 0.015) and ‐1154G/A (A vs. G: OR = 0.74, 95% CI = 0.62–0.89, P = 0.001; A/A vs. G/G: OR = 0.57, 95% CI = 0.37–0.87, P = 0.009; A/G vs. G/G: OR = 0.69, 95% CI = 0.53–0.89, P = 0.004 and A/A + A/G vs. G/G: OR = 0.66, 95% CI = 0.52–0.85, P = 0.001).ConclusionThis meta‐analysis showed the risk role of the ‐2578 polymorphism and the protective role of the ‐1154 polymorphism when the APOE є4 status was negative, suggesting that the two polymorphisms in the VEGF promoter may have opposing effects on AD risk in an APOE є4‐independent manner.