bluuebunny/crossref_metadata_2025_split huggingface dataset:
The canonical Hippo pathway components modulate the differentiation of lamina propria regulatory T cells and T helper 17-like regulatory T cells in mouse colitis
Authors: Ge Liuqing , Xu Min , Huang Meifang , Liu Shaoping , Zhou Zhidai , Xia Ziqin , Dong Shouquan , Zhao Qiu , Zhu Ruiping , Zhou Feng 
Published: July 2024

Abstract
Regulatory T cells (Tregs) ameliorate inflammatory bowel diseases. However, their plasticity is not completely understood. In this study using a mouse colitis model, Tregs and T helper 17 (Th17)-like Tregs were detected and sorted using flow cytometry, followed by transcriptome sequencing, real-time reverse transcription polymerase chain reaction, and flow cytometry to analyze the mRNA profiles of these cells. Treg plasticity was evaluated by in vitro differentiation assays. The immunosuppressive activities of Tregs and Th17-like Tregs were assessed in an adoptive transfer assay. We found Treg-derived Th17-like Tregs in inflamed colonic lamina propria (LP). LP Th17-like Tregs expressed higher Th17-related cytokines and lower immunosuppressive cytokines compared with LP Tregs. Notably, Tregs expressed higher Yes-associated protein 1 (YAP1) but lower transcriptional coactivator with PDZ-binding motif (TAZ) than Th17-like Tregs. Verteporfin-mediated inhibition of YAP1 activity enhanced Th17-like Treg generation, whereas IBS008739-induced TAZ activation did not affect Th17-like Treg generation. Besides, verteporfin enhanced while IBS008739 suppressed the differentiation of Th17-like Tregs into Th17 cells. Furthermore, YAP1 activated STAT5 signaling in Tregs, whereas YAP1 and TAZ activated STAT3 and STAT5 signaling in Th17-like Tregs. Compared with Tregs, Th17-like Tregs were less efficacious in ameliorating colitis. Therefore, YAP1 suppressed Treg differentiation into Th17-like Tregs. Both YAP1 and TAZ inhibited the differentiation of Th17-like Tregs into Th17 cells. Therefore, YAP1 and TAZ probably maintain the immunosuppressive activities of Tregs and Th17-like Tregs in colitis.
Extended period of selection for antimicrobial resistance due to recirculation of persistent antimicrobials in broilers
Authors: Swinkels Aram F , Berendsen Bjorn J A , Fischer Egil A J , Zomer Aldert L , Wagenaar Jaap A 
Published: July 2024

Abstract

Objectives
Antimicrobials can select for antimicrobial-resistant bacteria. After treatment the active compound is excreted through urine and faeces. As some antimicrobials are chemically stable, recirculation of subinhibitory concentrations of antimicrobials may occur due to coprophagic behaviour of animals such as chickens.

Methods
The persistence of three antimicrobials over time and their potential effects on antimicrobial resistance were determined in four groups of broilers. Groups were left untreated (control) or were treated with amoxicillin (unstable), doxycycline or enrofloxacin (stable). Antimicrobials were extracted from the faecal samples and were measured by LC-MS/MS. We determined the resistome genotypically using shotgun metagenomics and phenotypically by using Escherichia coli as indicator microorganism.

Results
Up to 37 days after treatment, doxycycline and enrofloxacin had concentrations in faeces equal to or higher than the minimal selective concentration (MSC), in contrast to the amoxicillin treatment. The amoxicillin treatment showed a significant difference (P ≤ 0.01 and P ≤ 0.0001) in the genotypic resistance only directly after treatment. On the other hand, the doxycycline treatment showed approximately 52% increase in phenotypic resistance and a significant difference (P ≤ 0.05 and P ≤ 0.0001) in genotypic resistance throughout the trial. Furthermore, enrofloxacin treatment resulted in a complete non-WT E. coli population but the quantity of resistance genes was similar to the control group, likely because resistance is mediated by point mutations.

Conclusions
Based on our findings, we suggest that persistence of antimicrobials should be taken into consideration in the assessment of priority classification of antimicrobials in livestock.
Regulating Tissue Growth Factors for Healing With Etherified Carboxymethylcellulose Matrix
Authors: Lin Guiting , Ramdas Shandilya , Hadid Hosam , Van Vleet Jared , Lue Tom F , Poulakidas Stathis 
Published: July 2024

Abstract
Etherified Carboxymethylcellulose Matrix (eCMC) is a revolutionary application of carboxymethylcellulose (CMC) in wound care, known for its potential in hemostasis and tissue regeneration. This study aims to investigate the mechanism of eCMC in tissue healing by establishing a rat burn model and administering eCMC as a treatment. The objective is to analyze cytokines and inflammatory mediators using a Cytokine Array and histochemical staining to understand the effects of eCMC on tissue regeneration. A rat burn model was created, and eCMC was applied as a treatment. Tissue samples were collected at multiple time points to assess the expression of cytokines and inflammatory mediators using a Cytokine Array. In addition, histochemical staining was performed to evaluate tissue regeneration factors. eCMC induced the expression of endogenous cytokines, particularly vascular epithelial growth factor and platelet-derived growth factor, while inhibiting inflammatory cytokines such as CINC-1, CINC-2, and MMP-8. This dual action facilitated wound healing and mitigated the risk of infection. eCMC demonstrates promising potential for enhancing skin regeneration. Further research is warranted to delve into the precise mechanism of eCMC’s cytokine regulation. In vitro and in vivo studies should be conducted to comprehensively investigate the therapeutic capabilities of eCMC in wound healing.
Secretory Carcinoma of the Breast: Radiologic-Pathologic Correlation
Authors: Boustros Pamela , Sanchez Lilia Maria , Gaboury Louis , El Khoury Mona 
Published: July 2024

Abstract
Secretory carcinoma is a rare, low-grade, special histological type of invasive breast carcinoma. Although it is the most common primary breast cancer in the pediatric population, most cases are diagnosed in adults, with a median age of 48 years (range 3 to 91 years). It most often presents as a painless and slowly growing palpable lump. Imaging findings are nonspecific. Secretory carcinomas have abundant periodic acid–Schiff positive intracytoplasmic and extracellular secretions on histopathology. Nearly all secretory carcinomas have mild to moderate nuclear pleomorphism with low mitotic activity. Over 80% (86/102) of secretory carcinomas display the translocation of t(12;15)(p13;q25), resulting in ETV6::NTRK3 gene fusion. Secretory carcinoma generally has an indolent course and has a better prognosis and overall survival than invasive breast carcinoma of no special type. A good prognosis is associated with age &lt;20 years, tumor size &lt;2 cm, and ≤3 axillary lymph node metastases. Metastases beyond the ipsilateral axillary lymph nodes are rare, with the most common sites involving the lung and liver. Except for the potential addition of targeted drug therapy for NTRK fusion–positive tumors, the treatment approach is otherwise similar to invasive breast carcinomas of similar receptor status.
Framework for Successful Integration of Health Services Research Into a Breast Imaging Career
Authors: Miles Randy C , Chou Shinn-Huey , Lamb Leslie R , Narayan Anand , Tran Nhat-Tuan , Lee Janie M 
Published: July 2024

Abstract
Health services research (HSR) is a multidisciplinary field of inquiry that examines how health care is structured, providing valuable data on health care outcomes and delivery. Over the past few decades, a shift in the U.S. health care system toward value-based care has placed a priority on health services topics. Health services research has been central to the evolution of breast imaging over this period, with increased emphasis placed on the following: (1) design of appropriate-use criteria for imaging services; (2) determination of cost-effectiveness of imaging protocols and screening regimens guiding policy; and (3) evaluation of policy related to reimbursement for diagnostic imaging and image-guided procedures. Examples of HSR topics that can be applied directly to breast imaging include evaluation of health care availability and accessibility, analysis of health care use patterns, exploration of patient preferences, assessment of technological innovation, development and implementation of clinical practice guidelines and screening strategies, and examination of health care organization and delivery models. Breast imaging radiologists who perform HSR are uniquely positioned to advocate for patients, to promote transformative health care interventions, and to influence policy changes and public health initiatives in breast imaging through analysis of health care data and translation of their research findings. In this Training and Professional Development article, we aim to provide practical approaches to explore interest in HSR and to describe a framework for successful integration of HSR into a breast imaging career.
Bone microarchitecture and strength assessed by HRpQCT in individuals with type 2 diabetes and prediabetes: the Maastricht study
Authors: Van Hulten Veerle , Sarodnik Cindy , Driessen Johanna H M , Viggers Rikke , Rasmussen Nicklas H , Geusens Piet P M M , Schaper Nicolaas , Schram Miranda T , De Galan Bastiaan E , Koster Annemarie , Bours Sandrine P G , Vestergaard Peter , Stehouwer Coen D A , van den Bergh Joop P 
Published: July 2024

Abstract
Type 2 diabetes (T2D) is a prevalent disease and has been associated with an increased fracture risk despite normal or even higher areal BMD. The aim of this study was to estimate the association between glucose metabolism status (GMS) and measurements of glycemic control with HRpQCT parameters of bone microarchitecture and strength. Participants of the Maastricht study who underwent an HRpQCT scan at the distal radius and tibia were included. GMS was determined by use of an oral glucose tolerance test and grouped into a normal glucose metabolism (NGM), prediabetes, or T2D. Linear regression models were used, stratified by sex with multiple adjustments. This study incorporated cross-sectional data from 1400 (796 [56.9%] NGM, 228 [16.3%] prediabetes, and 376 [26.9%] T2D) men and 1415 (1014 [71.7%] NGM, 211 [14.9%] prediabetes, and 190 [13.4%] T2D) women. The mean age was 59.8 ± 8.6 and 57.6 ± 9.0 yr for men and women, respectively. After adjustment, T2D was associated with a higher total BMD measured by HRpQCT and cortical thickness, and a smaller total and trabecular area in men and women compared with NGM. In women, T2D was additionally associated with a higher stiffness and failure load at the radius. Results were more pronounced at the distal radius than at the distal tibia. To conclude, these findings suggest that in this cohort of Maastricht study participants, total and trabecular bone area are smaller, but bone microarchitecture, density, and bone strength assessed by HRpQCT are not impaired in individuals with T2D.
Advances in inhaler therapy for asthma and chronic obstructive pulmonary disease: a comprehensive review of Fostair™ and Trimbow™
Authors: Foster Katie , Wong Chun Yuen Jerry 
Published: July 2024

Abstract
The management of asthma and chronic obstructive pulmonary disease (COPD) poses considerable challenges due to the intricate nature of these respiratory conditions. Fostair™ and Trimbow™, two pressurized metered dose inhalers, have emerged as noteworthy therapeutic options for treating both asthma and COPD. Fostair combines an inhaled corticosteroid, specifically beclometasone dipropionate, with a long-acting beta2-agonist, formoterol fumarate dihydrate, offering a dual-action approach to mitigate airway inflammation and bronchoconstriction. Conversely, Trimbow integrates a tri-particulate formulation consisting of beclometasone dipropionate, formoterol fumarate dihydrate, and glycopyrronium bromide, providing a comprehensive strategy to target the pathophysiology of COPD and asthma. Recent clinical trials have underscored Trimbow’s superior efficacy compared with Fostair, particularly in terms of reducing exacerbation rates and enhancing lung function. However, despite their therapeutic promise, both inhalers encounter challenges, including limited generalizability of study findings and a disparity between in vitro and human trial results. This literature review offers an in-depth analysis of Fostair and Trimbow, delving into their mechanisms of action, clinical applications, and outcomes in human studies for asthma and COPD. Additionally, the review discusses the role of combination therapy in managing respiratory diseases and underscores the necessity for further research to address existing knowledge gaps and optimize therapeutic outcomes.
The Relationship between the Military Medical Officer and Commanding Officer: Implications for Education and Training
Authors: Cole Rebekah , Crawford Kiia , Farrell Makinna , Vojta Leslie , Schwartz James , Rudinsky Sherri L 
Published: July 2024

ABSTRACT

Introduction
A successful working relationship between the Military Medical Officer (MMO) and Commanding Officer (CO) is critical for mission success. However, there is a gap in the professional literature outlining evidence-based practices for teaching early career MMOs how to strategically develop this relationship. The purpose of this study, therefore, was to explore our participants’ perceptions of how MMOs can foster a positive and productive working relationship with the CO.

Materials and Methods
We leveraged interpretive phenomenological design to interview 16 seasoned military physicians with extensive operational experience. We transcribed and coded each interview. Our research team determined how to organize these codes into major categories, which served as our study’s themes.

Results
The participants described three key elements for developing a good working relationship between the CO and MMO: (1) mission-focused communication; (2) active and physical presence; and (3) expert/advisor role fulfillment. The MMO should communicate with the CO in an understandable and mission-focused manner. The MMO should likewise be present within day-to-day operations and should establish their officership during mission planning. The MMO should fulfill their role as a subject matter expert, yet advisor, to the CO.

Conclusions
Communication, presence, and role fulfillment are essential elements for optimizing the MMO–CO working relationship. Learning these roles during medical school may enhance early career physicians’ mission readiness.
Efficient Digital Workflow to Replace a Missing Denture Tooth for Complete Denture Repair
Authors: Hu Eric , Paek Gen 
Published: July 2024

ABSTRACT
Debonded denture teeth is a common complication on a fixed or removable dental prosthesis when denture teeth are fabricated separately from the denture base and bonded together. Missing denture teeth can alter a person’s appearance, speech, or ability to eat. Traditionally, denture teeth repair can be a challenge for clinicians or dental laboratory technicians to find the identical tooth mould to meet esthetic and functional demands. This case report describes a digital workflow to scan, design, and mill a well-fitting and esthetic denture tooth to expedite treatment, improve patient satisfaction, and increase troop readiness when traditional methods may not be feasible.
Action and energy clustering of stellar streams in deforming Milky Way dark matter haloes
Authors: Brooks Richard A N , Sanders Jason L , Lilleengen Sophia , Petersen Michael S , Pontzen Andrew 
Published: July 2024

ABSTRACT
We investigate the non-adiabatic effect of time-dependent deformations in the Milky Way (MW) halo potential on stellar streams. Specifically, we consider the MW’s response to the infall of the Large Magellanic Cloud (LMC) and how this impacts our ability to recover the spherically averaged MW mass profile from observation using stream actions. Previously, action clustering methods have only been applied to static or adiabatic MW systems to constrain the properties of the host system. We use a time-evolving MW–LMC simulation described by basis function expansions. We find that for streams with realistic observational uncertainties on shorter orbital periods and without close encounters with the LMC, e.g. GD-1, the radial action distribution is sufficiently clustered to locally recover the spherical MW mass profile across the stream radial range within a $2\sigma$ confidence interval determined using a Fisher information approach. For streams with longer orbital periods and close encounters with the LMC, e.g. Orphan–Chenab (OC), the radial action distribution disperses as the MW halo has deformed non-adiabatically. Hence, for OC streams generated in potentials that include an MW halo with any deformations, action clustering methods will fail to recover the spherical mass profile within a $2\sigma$ uncertainty. Finally, we investigate whether the clustering of stream energies can provide similar constraints. Surprisingly, we find for OC-like streams, the recovered spherically averaged mass profiles demonstrate less sensitivity to the time-dependent deformations in the potential.
Shaping the nebula around the symbiotic system R Aquarii
Authors: Santamaría E , Toalá J A , Liimets T , Guerrero M A , Botello M K , Sabin L , Ramos-Larios G 
Published: July 2024

ABSTRACT
We present an analysis of high-dispersion spectroscopic observations of the symbiotic system R Aquarii (R Aqr) obtained with the Manchester Echelle Spectrograph at the 2.1-m telescope of the San Pedro Mártir Observatory (Mexico) in conjunction with available narrow-band images. The data are interpreted by means of the shape software to disclose the morpho-kinematics of the nebulosities associated with R Aqr. The model that best reproduces narrow-band images and position–velocity diagrams consists of three structures: an outer (large) hourglass structure surrounding an inner bipolar with a spiral-like filament entwined around the latter. The expansion velocity pattern of each structure is defined by different homologous expansion laws, which correspond to kinematic ages of $\tau _1$ = 450 $\pm$ 25 yr (outer hourglass), $\tau _2$ = 270 $\pm$ 20 yr (inner bipolar), and $\tau _3$ = 285$\pm$ 20 yr (spiral-like filament). We suggest that the spiral-like filament is tracing the regions of the interaction of a precessing jet with the circumstellar material, which simultaneously carves the inner bipolar structure. If a similar process created the large hourglass structure, it means that the action of the jet ceased for about 170 yr. We discuss the implications for other unresolved symbiotic systems detected in X-rays.
MeerKAT observations of starburst galaxies and AGNs within the core of XMMXCS J2215.9−1738 at z = 1.46
Authors: Klutse D Y , Hilton M , Heywood I , Smail I , Swinbank A M , Knowles K , Sikhosana S P 
Published: July 2024

ABSTRACT
We present the first detailed radio study of the galaxy cluster XMMXCS J2215.9−1738 at z = 1.46 using MeerKAT L-band (1.3 GHz) observations. We combine our radio observation with archival optical and infrared data to investigate the star formation and active galactic nucleus (AGN) population within $R_{200}$ ($R =$ 0.8 Mpc) of the cluster centre. Using three selection criteria; the radio luminosity, the far-infrared radio ratio ($q_{\rm {IR}}$), and the mid-infrared colour, we distinguish galaxies with radio emission predominantly powered by star formation from that powered by AGNs. We selected 24 cluster members within $R_{\rm {200}}$ in the MeerKAT image based on either their photometric or spectroscopic redshift. We classified 12/24 (50 per cent) as galaxies whose radio emission is dominated by star-formation activity, 6/24 (25 per cent) as intermediate star-forming galaxies, and 6/24 (25 per cent) as AGN-dominated galaxies. Using the radio continuum luminosities of the star-forming cluster galaxies, we estimated an integrated star formation rate (SFR) value of 1700 $\pm$ 330 M$_{\odot }$ yr$^{-1}$ within $R_{200}$. We derived a mass-normalized integrated SFR value of $(570 \pm 110) \times 10^{-14}$ yr$^{-1}$. This supports previous observational and theoretical studies that indicated a rapid increase in star formation activity within the core of high-redshift clusters. We also show that the high-AGN fraction within the cluster core is consistent with previous cluster observations at $z \gt $ 1.5.
“I’m standing next to him, I’m supporting him”—Supporting a loved one with brain cancer to donate their brain: A qualitative study
Authors: Griffin Cassandra P , Carlson Melissa A , Walker Marjorie M , Lynam James , Paul Christine L 
Published: July 2024

Abstract

Background
Brain cancer is a devastating and incurable disease that places a high burden of care on next of kin (NOK). NOK can play a core role in supporting end-of-life planning, including the decision to donate one’s brain after death. Postmortem brain donation is crucial to research. As postmortem programs develop it is important to understand the experiences of NOK as they support a loved one in the donation decision.

Methods
Thirteen qualitative interviews were completed with NOK of people who had consented to donate their brains to the Mark Hughes Foundation (MHF) Biobank. A thematic analysis was carried out on the transcribed interviews.

Results
Four central themes were identified: (i) The carer role has additional responsibilities and psychological benefits when brain donation is being considered; (ii) Supporting a loved one to donate requires mutual trust, understanding, and a commitment to honor agency; (iii) Increasing awareness of brain donation is a priority for NOK, and (iv) Brain donation is seen as a natural continuation of the donor’s altruistic values.

Conclusions
When a person with brain cancer decides to donate their brain to research, their NOK can experience additional burdens and benefits as the NOK–patient relationship evolves. Understanding this evolution and recognizing the importance of trust, advocacy, and altruism provides a guide for the integration of brain donation programs into clinical pathways and a basis for normalizing brain donation as an extension of organ donation frameworks.
Rescue of dead MnO2 for stable electrolytic Zn–Mn redox-flow battery: a metric of mediated and catalytic kinetics
Authors: Wang Qi , Zhou Wanhai , Zhang Yanyan , Jin Hongrun , Li Xinran , Zhang Tengsheng , Wang Boya , Zhao Ruizheng , Zhang Junwei , Li Wei , Qiao Yu , Jia Chuankun , Zhao Dongyuan , Chao Dongliang 
Published: July 2024

ABSTRACT
The virtues of electrolytic MnO2 aqueous batteries are high theoretical energy density, affordability and safety. However, the continuous dead MnO2 and unstable Mn2+/MnO2 electrolysis pose challenges to the practical output energy and lifespan. Herein, we demonstrate bifunctional cationic redox mediation and catalysis kinetics metrics to rescue dead MnO2 and construct a stable and fast electrolytic Zn–Mn redox-flow battery (eZMRFB). Spectroscopic characterizations and electrochemical evaluation reveal the superior mediation kinetics of a cationic Fe2+ redox mediator compared with the anionic ones (e.g. I– and Br–), thus eliminating dead MnO2 effectively. With intensified oxygen vacancies, density functional theory simulations of the reaction pathways further verify the concomitant Fe-catalysed Mn2+/MnO2 electrolysis kinetics via charge delocalization and activated O 2p electron states, boosting its rate capability. As a result, the elaborated eZMRFB achieves a coulombic efficiency of nearly 100%, ultra-high areal capacity of 80 mAh cm–2, rate capability of 20 C and a long lifespan of 2500 cycles. This work may advance high-energy aqueous batteries to next-generation scalable energy storage.