bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic identification of early salicylate- and flg22-responsive redox-sensitive proteins in Arabidopsis
Authors: Liu Pei , Zhang Huoming , Yu Boying , Xiong Liming , Xia Yiji 
Published: February 2015

AbstractAccumulation of reactive oxygen species (ROS) is one of the early defense responses against pathogen infection in plants. The mechanism about the initial and direct regulation of the defense signaling pathway by ROS remains elusive. Perturbation of cellular redox homeostasis by ROS is believed to alter functions of redox-sensitive proteins through their oxidative modifications. Here we report an OxiTRAQ-based proteomic study in identifying proteins whose cysteines underwent oxidative modifications in Arabidopsis cells during the early response to salicylate or flg22, two defense pathway elicitors that are known to disturb cellular redox homeostasis. Among the salicylate- and/or flg22-responsive redox-sensitive proteins are those involved in transcriptional regulation, chromatin remodeling, RNA processing, post-translational modifications and nucleocytoplasmic shuttling. The identification of the salicylate-/flg22-responsive redox-sensitive proteins provides a foundation from which further study can be conducted toward understanding biological significance of their oxidative modifications during the plant defense response.
Regulation of Primary Metabolic Pathways in Oyster Mushroom Mycelia Induced by Blue Light Stimulation: Accumulation of Shikimic Acid
Authors: Kojima Masanobu , Kimura Ninako , Miura Ryuhei 
Published: February 2015

AbstractShikimic acid is a key intermediate in the aromatic amino acid pathway as well as an important starting material for the synthesis of Tamiflu, a potent and selective inhibitor of the neuraminidase enzyme of influenza viruses A and B. Here we report that in oyster mushroom (Pleurotus ostreatus) mycelia cultivated in the dark, stimulation with blue light-emitting diodes induces the accumulation of shikimic acid. An integrated analysis of primary metabolites, gene expression and protein expression suggests that the accumulation of shikimic acid caused by blue light stimulation is due to an increase in 3-deoxy-D-arabinoheptulosonate 7-phosphate synthase (DAHPS, EC2.5.1.54), the rate-determining enzyme in the shikimic acid pathway, as well as phosphofructokinase (PFK, EC2.7.1.11) and glucose-6-phosphate dehydrogenase (G6PD, EC1.1.1.49), the rate-determining enzymes in the glycolysis and pentose phosphate pathways, respectively. This stimulation results in increased levels of phosphoenolpyruvic acid (PEP) and erythrose-4-phosphate (E4P), the starting materials of shikimic acid biosynthesis.
Defining the cytosolic pathway of glutathione degradation in Arabidopsis thaliana: role of the ChaC/GCG family of γ-glutamyl cyclotransferases as glutathione-degrading enzymes and AtLAP1 as the Cys-Gly peptidase
Authors: Kumar Shailesh , Kaur Amandeep , Chattopadhyay Banani , Bachhawat Anand K. 
Published: February 2015

Glutathione homoeostasis is critical to plant life and its adaptation to stress. The γ-glutamyl cycle of glutathione biosynthesis and degradation plays a pre-eminent role in glutathione homoeostasis. The genes encoding two enzymatic steps of glutathione degradation, the γ-glutamyl cyclotransferase (GGCT; acting on γ-glutamyl amino acids) and the Cys-Gly dipeptidase, have, however, lacked identification. We have investigated the family of GGCTs in Arabidopsis thaliana. We show through in vivo functional assays in yeast that all three members of the ChaC/GCG subfamily show significant activity towards glutathione but no detectable activity towards γ-glutamyl methionine. Biochemical characterization of the purified recombinant enzymes GGCT2;2 and GGCT2;3 further confirmed that they act specifically to degrade glutathione to yield 5-oxoproline and Cys-Gly peptide and show no significant activity towards γ-glutamyl cysteine. The Km for glutathione was 1.7 and 4.96 mM for GGCT2;2 and GGCT2;3 respectively and was physiologically relevant. Evaluation of representative members of other subfamilies indicates the absence of GGCTs from plants showing significant activity towards γ-glutamyl-amino acids as envisaged in the classical γ-glutamyl cycle. To identify the Cys-Gly peptidase, we evaluated leucine aminopeptidases (LAPs) as candidate enzymes. The cytosolic AtLAP1 (A. thaliana leucine aminopeptidase 1) and the putative chloroplastic AtLAP3 displayed activity towards Cys-Gly peptide through in vivo functional assays in yeast. Biochemical characterization of the in vitro purified hexameric AtLAP1 enzyme revealed a Km for Cys-Gly of 1.3 mM that was physiologically relevant and indicated that AtLAP1 represents a cytosolic Cys-Gly peptidase activity of A. thaliana. The studies provide new insights into the functioning of the γ-glutamyl cycle in plants.
Using a Laboratory‐Based Growth Model to Estimate Mass‐ and Temperature‐Dependent Growth Parameters across Populations of Juvenile Chinook Salmon
Authors: Perry Russell W. , Plumb John M. , W. Huntington Charles 
Published: February 2015

AbstractTo estimate the parameters that govern mass‐ and temperature‐dependent growth, we conducted a meta‐analysis of existing growth data from juvenile Chinook Salmon Oncorhynchus tshawytscha that were fed an ad libitum ration of a pelleted diet. Although the growth of juvenile Chinook Salmon has been well studied, research has focused on a single population, a narrow range of fish sizes, or a narrow range of temperatures. Therefore, we incorporated the Ratkowsky model for temperature‐dependent growth into an allometric growth model; this model was then fitted to growth data from 11 data sources representing nine populations of juvenile Chinook Salmon. The model fit the growth data well, explaining 98% of the variation in final mass. The estimated allometric mass exponent (b) was 0.338 (SE = 0.025), similar to estimates reported for other salmonids. This estimate of b will be particularly useful for estimating mass‐standardized growth rates of juvenile Chinook Salmon. In addition, the lower thermal limit, optimal temperature, and upper thermal limit for growth were estimated to be 1.8°C (SE = 0.63°C), 19.0°C (SE = 0.27°C), and 24.9°C (SE = 0.02°C), respectively. By taking a meta‐analytical approach, we were able to provide a growth model that is applicable across populations of juvenile Chinook Salmon receiving an ad libitum ration of a pelleted diet.Received May 20, 2014; accepted December 4, 2014
Effects of phytochemicals on in vitro anti-inflammatory activity of Bifidobacterium adolescentis
Authors: Kawabata Kyuichi , Kato Yuri , Sakano Taiken , Baba Nobuyuki , Hagiwara Kota , Tamura Akira , Baba Seigo , Natsume Midori , Ohigashi Hajime 
Published: February 2015

Abstract
Probiotics have been shown to improve the condition of not only the human gastrointestinal tract but also the entire body. We found that quercetin enhances the anti-inflammatory activity of Bifidobacterium adolescentis, which is abundant in human intestines. Here, we assessed whether certain phytochemicals could enhance the anti-inflammatory activity of B. adolescentis. Bifidobacteria were anaerobically cultured with phytochemicals for 3 h, and the anti-inflammatory activity of the supernatants was estimated by testing their ability to inhibit nitric oxide (NO) production by lipopolysaccharide-stimulated RAW264 macrophages. Of the 55 phytochemicals tested, phloretin, (+)-taxifolin, and (−)-epigallocatechin gallate as well as quercetin-3-O-glucoside and quercetin-4′-O-glucoside were similar to quercetin in promoting NO suppression by B. adolescentis. In addition, the phytochemicals excluding quercetin increased the concentrations of lactic and acetic acids in the co-culture supernatants. These results suggest that some phytochemicals may activate the anti-inflammatory function of B. adolescentis.
Introduction of an antifungal stewardship programme targeting high-cost antifungals at a tertiary hospital in Cambridge, England
Authors: Micallef C. , Aliyu S. H. , Santos R. , Brown N. M. , Rosembert D. , Enoch D. A. 
Published: February 2015

Abstract

Background
Antifungal stewardship aims to promote the optimal use of antifungals through the careful selection of agents based on patient profile, target organism, toxicity, costs and the likelihood of emergence and spread of resistance.

Methods
We report on an observational prospective 12 month study conducted by an antifungal stewardship team targeting the use of echinocandins (caspofungin and micafungin), voriconazole and liposomal amphotericin B in a tertiary referral hospital in the UK.

Results
One-hundred-and-seventy-three patients were reviewed on 294 occasions. Clinical advice was given and implemented during review of 45 (88.2%) of micafungin prescriptions, 70 (78.7%) of those receiving voriconazole, 78 (62.4%) of those receiving liposomal amphotericin B and 3 (27.3%) of those receiving caspofungin. Except for voriconazole, nearly half of all treatments reviewed were stopped or changed. This study found that a crude cost saving of ∼£180 000 in antifungal drugs was generated compared with the previous year.

Conclusions
Using a multidisciplinary team, antifungal stewardship can achieve significant improvements in patient management and it may reduce costs.
Sex hormones in male psoriasis patients and their correlation with the Psoriasis Area and Severity Index
Authors: Cemil Bengu C. , Cengiz Fatma P. , Atas Hatice , Ozturk Gulfer , Canpolat Filiz 
Published: February 2015

AbstractModulation of psoriasis severity by estradiol during pregnancy, menstruation and menopause has been investigated previously. The correlation between sex hormones and Psoriasis Area Severity Index (PASI) has not been studied in male psoriasis patients. We investigated serum sex hormones in male psoriasis patients compared with healthy controls and correlated these findings with PASI. Estradiol, testosterone, follicle‐stimulating hormone (FSH) and luteinizing hormone (LH) levels were measured in 47 male patients with psoriasis and 20 healthy controls. Patients with psoriasis showed higher body mass index and higher serum levels of FSH and LH relative to healthy controls, although this difference was not statistically significant. However, serum levels of testosterone and estradiol were significantly different between patients with psoriasis and healthy controls. Testosterone was significantly increased in control patients and estradiol was significantly increased among psoriatic patients. A significant inverse correlation was found between estradiol and PASI. Although the role of sex hormones in the pathogenesis of psoriasis has not been demonstrated, this is the first report of an inverse correlation between estradiol and PASI in male patients.
Association of anti‐RNA polymerase III antibody and malignancy in Japanese patients with systemic sclerosis
Authors: Saigusa Ryosuke , Asano Yoshihide , Nakamura Kouki , Miura Shunsuke , Ichimura Yohei , Takahashi Takehiro , Toyama Tetsuo , Taniguchi Takashi , Noda Shinji , Aozasa Naohiko , Akamata Kaname , Sumida Hayakazu , Miyazaki Miki , Tamaki Zenshiro , Yanaba Koichi , Kuwano Yoshihiro , Sato Shinichi 
Published: February 2015

AbstractPatients with systemic sclerosis (SSc) have an increased risk of malignancy compared with the general population. Recently, SSc patients with anti‐RNA polymerase III antibody have been reported to have an increased risk of malignancy as compared with those with other disease‐specific autoantibodies in US, European and Australian populations. Therefore, we studied the relationship between disease‐specific autoantibodies and malignancy in 261 Japanese SSc patients. The prevalence of malignancy was significantly higher in patients with anti‐RNA polymerase III antibody (7/22, 31.8%) than in those with anti‐topoisomerase I antibody (2/82, 2.4%) and in those with anticentromere antibody (8/137, 5.8%). Importantly, among seven patients with anti‐RNA polymerase III antibody and malignancy, three patients (42.9%) developed malignancy from 6 months before to 12 months after SSc onset. Thus, malignancy complication in Japanese SSc patients with anti‐RNA polymerase III antibody is as high as that in other races, suggesting that SSc patients with anti‐RNA polymerase III antibody share the same pathological process among different ethnic groups.
Mobile Communication and Local Information Flow: Evidence from Distracted Driving Laws
Authors: BROWN NERISSA C. , STICE HAN , WHITE ROGER M. 
Published: February 2015

ABSTRACTWe examine the influence of mobile communication on local information flow and local investor activity using the enforcement of statewide distracted driving restrictions, which are exogenous events that constrain mobile communication while driving. By restricting mobile communication across a potentially sizable set of local individuals, these restrictions could inhibit local information flow and, in turn, the market activity of stocks headquartered in enforcement states. We first document a decline in Google search activity for local stocks when restrictions take effect, suggesting that constraints on mobile communication significantly affect individuals’ information search activity. We further find significant declines in local trading volume when restrictions are enforced. This drop in liquidity is (1) attenuated when laws provide substitutive means of mobile communication and (2) magnified when locals have long car commutes and when their daily commutes overlap with regular exchange hours. Moreover, trading volume suffers the most for local stocks with lower institutional ownership, less analyst coverage, and more intangible information. Additional analyses show lower intraday volume during local commute times when mobile connectivity is constrained. Together, our results suggest that local information and local investors matter in stock markets and that mobile communication is an important mechanism through which these elements operate to affect liquidity and price discovery.
Design of an Optimum Computer Vision‐Based Automatic Abalone (Haliotis discus hannai) Grading Algorithm
Authors: Lee Donggil , Lee Kyounghoon , Kim Seonghun , Yang Yongsu 
Published: February 2015

AbstractAn automatic abalone grading algorithm that estimates abalone weights on the basis of computer vision using 2D images is developed and tested. The algorithm overcomes the problems experienced by conventional abalone grading methods that utilize manual sorting and mechanical automatic grading. To design an optimal algorithm, a regression formula and R2 value were investigated by performing a regression analysis for each of total length, body width, thickness, view area, and actual volume against abalone weights. The R2 value between the actual volume and abalone weight was 0.999, showing a relatively high correlation. As a result, to easily estimate the actual volumes of abalones based on computer vision, the volumes were calculated under the assumption that abalone shapes are half‐oblate ellipsoids, and a regression formula was derived to estimate the volumes of abalones through linear regression analysis between the calculated and actual volumes. The final automatic abalone grading algorithm is designed using the abalone volume estimation regression formula derived from test results, and the actual volumes and abalone weights regression formula. In the range of abalones weighting from 16.51 to 128.01 g, the results of evaluation of the performance of algorithm via cross‐validation indicate root mean square and worst‐case prediction errors of are 2.8 and ±8 g, respectively.
Dissemination of Enterococcus faecalis and Enterococcus faecium in a Ricotta Processing Plant and Evaluation of Pathogenic and Antibiotic Resistance Profiles
Authors: Fernandes Meg da Silva , Fujimoto Graciela , de Souza Leandro Pio , Kabuki Dirce Yorika , da Silva Márcio José , Kuaye Arnaldo Yoshiteru 
Published: February 2015

AbstractIn this work, the sources of contamination by Enterococcus spp. in a ricotta processing line were evaluated. The isolated strains were tested for virulence genes (gelE, cylA,B, M, esp, agg, ace, efaA, vanB), expression of virulence factors (hemolysin and gelatinase), and the resistance to 10 different antibiotics. Enterococcus faecium and Enterococcus faecalis were subjected to discriminatory identification by intergenic spacer region (ITS)‐polymerase chain reaction and sequencing of the ITS region. The results showed that Enterococcus spp. was detected in the raw materials, environment samples and the final product. None of the 107 Enterococcus isolates were completely free from all virulence genes considered. A fraction of 21.5% of the isolates containing all of the genes of the cylA, B, M operon also expressed β‐hemolysis. Most of the isolates showed the gelE gene, but only 9.3% were able to hydrolyze gelatin. In addition, 23.5% of the observed Enterococcus isolates had the vanB gene but were susceptible to vancomycin in vitro. The dissemination of antibiotic‐resistant enterococci was revealed in this study: 19.3% of the E. faecium samples and 78.0% of the E. faecalis samples were resistant to at least one of the antibiotics tested. Sequencing of region discriminated 5 and 7 distinct groups among E. faecalis and E. faecium, respectively. Although some similarity was observed among some of the isolates, all E. faecalis and E. faecium isolates had genetic differences both in the ITS region and in the virulence profile, which makes them different from each other.
Implementing robotic surgery to gynecologic oncology: the first 300 operations performed at a tertiary hospital
Authors: Mäenpää Minna , Nieminen Kari , Tomás Eija , Luukkaala Tiina , Mäenpää Johanna U. 
Published: February 2015

AbstractObjectiveTo investigate the initial experience with robotic‐assisted laparoscopic surgery in gynecologic oncology.DesignA retrospective survey.SettingTertiary referral center.PopulationThe first 300 patients operated on using robotic assistance at the Department of Obstetrics and Gynecology of Tampere University Hospital, from March 2009 through January 2013.MethodsRetrospective patient chart review.Main outcome measuresThe primary outcome measure was the learning curve events, and the complication and conversion rates were secondary outcome measures.ResultsThe commonest type of operation was hysterectomy, bilateral salpingoophorectomy and pelvic lymphadenectomy (LH + BSO + PLND, n = 89), followed by the same procedure amended by para‐aortic lymphadenectomy (PALND, n = 74), type II radical hysterectomy (n = 24), and PLND + PALND ± omentectomy (n = 15). A learning curve was most evident for LH + BSO + PLND: whereas the median operative time of all 89 operations was 167 min (range 403–104), it was 260 (range 403–135) and 153 (range 247–104) min in the case of the first and last 20 operations, respectively. The learning curve was short, or 10 procedures. A learning curve was also seen for the preoperative time in the operation room and for the number of lymph nodes harvested. The median blood loss during all 300 operations was 100 mL (range 5–3200). The median postoperative hospital stay was 1 day. The conversion rate was 4.0%, and the complication rate 19.3% (major in 9%).ConclusionThe learning curve of robotic‐assisted laparoscopic surgery appears to be short, or 10 operations. Robotic‐assisted procedures seem to offer a safe and useful alternative to traditional techniques.
Chorioretinal scars and visual deprivation are common in children with cochlear implants after congenital cytomegalovirus infection
Authors: Teär Fahnehjelm Kristina , Olsson Monica , Fahnehjelm Cecilia , Lewensohn‐Fuchs Ilona , Karltorp Eva 
Published: February 2015

AbstractAimThe aim of this study was to compare visual function and ocular characteristics in children with cochlear implants, due to severe hearing impairment caused by the congenital cytomegalovirus (CMV) infection, with control children fitted with cochlear implants due to connexin 26 mutations (Cx26), a genetic cause of hearing impairment.MethodsWe carried out ophthalmological assessments, including visual acuity, ocular alignment, Ocular Motor Score, biomicroscopy and fundus photography, on 26 children with congenital CMV (median age 8.3 years, range 1.4–16.7) and 13 Cx26 controls (median age 5.6 years, range 1.7–12.5).ResultsWe found unilateral chorioretinal macular scars that reduced best‐corrected visual acuity ≤0.3 in five (19%) of the children with congenital CMV, but in none of the children with Cx26 (p = 0.15). Ocular motility problems were more common among children with congenital CMV, but the difference was not significant (p = 0.20). The vestibulo‐ocular reflex was more frequently pathological in children with congenital CMV (p = 0.011).ConclusionOcular complications with central chorioretinal scars and ocular motility disturbances were common in children treated with cochlear implants due to severe hearing impairment caused by the congenital CMV infection. Ophthalmological assessments are advisable in such children for early identification, intervention and follow‐up.
Indications and practical implementation of microbiologic diagnostics in patients with chronic wounds
Authors: Schwarzkopf Andreas , Dissemond Joachim 
Published: February 2015

SummaryMicrobiology diagnostics are frequently performed in patients with chronic wounds. However, there is currently a lack of uniformity with respect to indications as well as the practical implementation of such workup. The fact that diagnostic results may be significantly affected by the sampling technique used as well as the preceding (wound) preparation underscores the need for uniform standards, which have been missing so far.In Germany, bacteriologic wound swabs are routinely performed, particularly with the intent to screen for multiresistant pathogens. For this indication, prior wound cleansing should be avoided, and sampling using the Essen Rotary technique provides a quick and easy‐to‐use option. If there is clinical suspicion of an infection, wound cleansing with sterile saline solution (0.9 %) and/or sterile cotton gauze should be carried out prior to obtaining bacteriologic swabs. While routine diagnostic biopsies are generally not required in chronic wound patients, they may be useful in case of clinically suspected wound infections, particularly in patients with deep ulcerations, diabetic foot syndrome, severe soft tissue infection, or fistula tissue. Moreover, biopsies are indispensable in the microbiology workup of specific pathogens such as mycobacteria, Leishmania, actinomycetes, Nocardia ssp. or molds.