bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibiotic Profiling of Staphylococcus Aureus and Genotypic Detection of Ermgene Causing Inducible Resistance to Clindamycin Isolated From All Clinical Samples in a Tertiary Care Center
Authors: Sharma Ananya , Leela K.V. , V Jagan. 
Published: June 2024

Abstract

Objective
The objective of this investigation is to ascertain the phenotypic and genotypic expression of inducible clindamycin resistance in clinical isolates of methicillin-resistant Staphylococcus aureus (MRSA) as a result of the expression of ermA, ermB, and ermC genes using and double disc diffusion method and RT-PCR.
Method
This cross-sectional study was conducted at department of Microbiology, SRM Medical College Hospital & Research Centre. Total of 150 isolates of S. aureus were collected. All these isolates were cultured aerobically. Staphylococcus aureus was identified and Antimicrobial Susceptibility Testing (AST) was done using Kirby-Bauer disk diffusion method and inducible resistance to clindamycin is detected by “D-test”. All clinical isolates of MRSA during the study period were included
Result
Analysis of 150 S. aureus isolates revealed 60 MRSA. Out of which 31 were detected as inducible clindamycin by double disk diffusion method. These 31 isolates were subjected to RT-PCR for genotypic detection of erm gene. 13 were found to be ermC, 5 were found to be ermB and 3 were found to be ermA positive isolates.
Conclusion
Clindamycin may be a viable alternative and should be recommended for severe MRSA infections, as indicated by its susceptibility pattern. We observed the D test as a mandatory method for the detection of inducible clindamycin-resistant Staphylococcus. It is crucial to note that the ermC gene is a significant determinant of macrolide resistance in MRSA.
Silencing SODB1: A Novel Approach to Suppress Leishmania tropica Parasite Infectivity
Authors: Alhraki Fatema , Kassouha Morshed , Soukkarieh Chadi 
Published: June 2024

Abstract

Introduction: Recent research has focused on genetic modifications and manipulations, which address the root causes rather than just the outcomes. These methods are now recognized as powerful tools for studying Leishmania parasites’ biology. In this study, the sodb1 gene was targeted using antisense RNA. This gene encodes an essential enzyme, superoxide dismutase (SODB1), which plays a crucial role in the infectivity of Leishmania tropica parasites within macrophages.
Methods: The inverted sequence of a part of the sodb1 ORF and 3’UTR was cloned into the LEXSY plasmid. After obtaining the knock-down constructions, Leishmania tropica parasites were transfected using electroporation. Western blot analysis was performed to study and evaluate SODB1 expression and the infectivity of these mutant parasites in human macrophages, comparing them with a wild-type negative control and another control containing the EGFP gene, which codes for the enhanced Green Fluorescent Protein (EGFP).
Results: The use of LEXSY plasmids for sodb1 gene knock-down was efficient, resulting in a clear decrease in both infectivity and parasite load in human macrophages in vitro. Western blot analysis revealed a lower expression level of SODB1 in the mutant parasites compared to the wild- type. Statistical analysis confirmed that the decreases in infectivity and parasite load of the mutant parasites were highly significant compared to the wild-type. Statistical analysis confirmed that the decreases in infectivity and parasite load of the mutant parasites were highly significant compared to the wild-type.
Conclusion: Our study confirms the efficiency of the produced knock-down system and the importance of the SODB1 enzyme in the amastigote parasites’ ability to grow and survive within the host's macrophages. This is the first study that affirms the success of using the LEXSY gene expression system for gene knock-down according to the antisense RNA approach. This work will allow for the use of the produced knock-down system to target other important genes in Leishmania and continue studying the knocked-down strain in vivo.
"Editorial 181 enero junio 2024"
Authors: Peñaranda Quintana Mario Andrés , Púa Mora Franklin Giovanni 
Published: June 2024

En la Editorial Franciscanum 181 se rinde un homenaje a la memoria del Dr Enrique Domingo Dussel Ambrosini, filósofo y teólogo latinoamericano. Posteriormente se presentan los ocho artículos de este número de la revista.
Mitochondria and Lipid Droplets: Focus on the Molecular Structure
of Contact Sites in the Pathogenesis of Metabolic Syndrome
Authors: Litvinova Larisa , Vulf Maria , Yurova Kristina , Khaziakhmatova Olga , Malashchenko Vladimir , Bograya Maria , Kozlov Ivan , Todosenko Natalia 
Published: June 2024

Abstract:
Metabolic syndrome (MetS) is a complex of serious pathologies with a high
prevalence worldwide. Disruption of mitochondrial biogenesis and its interaction with
other cell organelles plays an important role in the development of MetS. Studies have
revealed the phenotypic and functional heterogeneity of mitochondria that exist within a
single cell and can regulate metabolic signaling pathways, influencing the development
of metabolic diseases. Excessive intake of fatty acids leads to changes in fatty acid
metabolism that affect the biology of important cell organelles - the lipid droplets,
whose specific biology is not fully understood. Perhaps targeted molecular genetic stimulation aimed at regulating the contact between mitochondria and lipids can break the vicious cycle of inflammation in MetS and restore normal cell function, reducing the risk
of developing concomitant pathologies. The review describes potential (promising) therapeutic molecular targets associated with mitochondria and lipid droplets, focusing on
the proteins involved in their contact and emphasizing their role in the pathogenesis of
MetS.
Electromagnetic Energy Harvesting During Pneumatic Cylinder Motion
Authors: Páez Luis Miguel Ruiz , Lei Liu , Bowen Jiang , Yifan Jia , Jie Wu , Chenwei Pu , Pengfei Qian 
Published: June 2024

Background:
Energy harvesting systems provide a sustainable way to capture and convert various forms of energy into usable electrical power. The Electromagnetic Energy Harvester
(EMEH) systems function based on Faraday’s law and are usually implemented in small systems
where the demand for electrical energy is low.

Objective:
This study aims to expand the use of a pneumatic cylinder by enabling it to work as an
EMEH.

Methods:
The objective can be achieved by adding a coil arrangement wrapped around the pneumatic cylinder and taking advantage of the rectilinear motion of the cylinder's inner magnet, which
is a moving magnetic field. The paper explored the influence of key parameters on the induced
voltage through a series of simulations of several designs in COMSOL software.

Results:
The simulation results of the final model show that the obtained induced voltage can be
effectively improved by optimizing the main parameters of the coil within a certain range. When
the coil is made up of a coil wire with a diameter of 0.4 mm, wound into 1000 turns, the induced
peak voltage obtained is 3.22 V, which represents a significant improvement over the initial design
parameters.

Conclusion:
Furthermore, the simulation shows that the optimized model is capable of achieving
an effective voltage of 922 mV. This demonstrates the impact of the key design parameters on the
system output, thus confirming the potential application value of enhanced pneumatic cylinders
with energy harvesting capabilities.
Development and Validation of a Mobile-Centered Digital Health Readiness Scale (mDiHERS): Health Literacy and Equity Scale
Authors: Kim Hana , Schnall Rebecca , Yoon Nagyeom , Koh Seong-Joon , Lee Jisan , Cheon Jae Hee 
Published: June 2024

Background
There has been a rapid expansion of digital health care services, making the need for measuring and improving digital health readiness a priority. In response, our study team developed the Mobile-Centered Digital Health Readiness: Health Literacy and Equity Scale (mDiHERS) to measure digital health readiness.

Objective
We aim to develop and validate a scale that assesses digital health readiness, encompassing literacy and equity, and to ensure the effective use of mobile-centered digital health services.

Methods
This study was conducted from October 2021 to October 2022 to develop and validate the mDiHERS. Participants included patients with inflammatory bowel disease, which is a chronic condition requiring continuous management, and experts in medical and nursing informatics. The scale development involved a literature review, focus group interviews, and content validity evaluations. A total of 440 patients with inflammatory bowel disease were recruited for the validation phase, with 403 completing the survey. The scale’s validity and reliability were assessed through exploratory factor analysis and Cronbach α. The scale was translated into English by translators and bilingual and native researchers, ensuring its applicability in diverse settings.

Results
The mDiHERS consists of 36 items across 6 domains, with a 5-point Likert scale for responses. The validation process confirmed the scale’s construct validity, with 4 factors explaining 65.05% of the total variance. The scale’s reliability was established with Cronbach α values ranging from 0.84 to 0.91. The scale’s development considered the technical proficiency necessary for engaging with health mobile apps and devices, reflecting the importance of subjective confidence and objective skills in digital health literacy.

Conclusions
The mDiHERS is a validated tool for measuring patients’ readiness and ability to use digital health services. The mDiHERS assesses user characteristics, digital accessibility, literacy, and equity to contribute to the effective use of digital health services and improve accessibility. The development and validation of the mDiHERS emphasize the importance of confidence and competence in managing health digitally. Continuous improvements are necessary to ensure that all patients can benefit from digital health care.
Alteraciones morfométricas en hembras juveniles de iguana negra (Ctenosaura pectinata) cuando varía el consumo de proteína y energía
Authors: Sánchez Mendez Cristina Guadalupe , López Pozos Roberto , Santiago Romero Héctor , Martínez García José Antonio , Mendoza Martínez Germán David , Arcos-García José Luis 
Published: June 2024

Como animal ectotermo, la iguana negra (Ctenosaura pectinata) come alimentos diversos y su crecimiento no es constante; por lo tanto, su ganancia de peso y crecimiento longitudinal son bajos. En esta investigación se estudiaron los cambios morfométricos de la iguana negra a temperatura ambiente. Se probaron diferentes porciones de proteína cruda y energía metabolizable. Se utilizaron 30 hembras juveniles con peso promedio de 124.4 ± 61.1 g. A cada hembra se le mantuvo en una jaula individual con un bebedero y un comedero semiautomáticos. Su alimentación consistió en la combinación de proteína cruda (29.4 y 33.4 %) y tres variantes de energía metabolizable: 2.49, 2.55 y 2.7 Mcal/kg. Las variables evaluadas fueron peso vivo (mg), perímetro, diámetro y longitud corporal (mm). Las variables asociadas al consumo de alimento fueron: materia seca (mg), proteína cruda (PC) y energía metabolizable (EM). Se empleó un diseño de bloques completos aleatorizados con arreglo factorial 2×3, donde el criterio de bloqueo fue el peso de las iguanas. Para observar las diferencias entre tratamientos, se utilizó la prueba de Tukey. Hubo diferencias (P < 0.0500) en el ancho de la cabeza y el diámetro base de la cola por efecto del factor energía. Los tratamientos de 29.4 PC y 2.55 EM, y 33.4 PC y 2.55 EM mostraron mayor (P < 0.0500) consumo de materia seca, proteína y energía. Debido a la alimentación también se registró aumento en algunas variables morfométricas. Las hembras juveniles crecieron mejor con la combinación alimenticia de: 33.4 y 2.55 (PC−EM).
ANALISIS PUTUSAN HAKIM NO. 319/PDT.G/2017 PADA GUGATAN WANPRESTASI UNTUK PELAKSANAAN KONTRAK MURABAHAH BI AL-WAKALAH
Authors: Maulana Muhammad 
Published: June 2024

Penerapan murabahah bi al-wakalah yang dilakukan oleh bank BRI Syariah cabang Banda Aceh telah menimbulkan persengketaan disebabkan pihak nasabah tidak menerima sepenuhnya perwakilan untuk pembelian rumah yang telah diwakalahkan pada akad pembiayaan. Hal tersebut telah menimbulkan konflik antara pihak bank, nasabah dan developer terjadi permasalahan. Adapun tujuan penelitian skripsi ini yaitu untuk meneliti pendapat hakim Mahkamah Syar’iyah Kota Banda Aceh putusan No.319/Pdt.G/2017 dalam menolak gugatan pengggugat dan juga menolak eksepsi tergugat, serta menganalisis pertimbangan hakim dalam gugatan wanprestasi pada pembiayaan murabahah bi al-wakalah pada BRI Syariah KCP Banda Aceh. Untuk mendapatkan data yang objektif serta valid, penulis menggunakan desain penelitian dengan pendekatan yuridis normatif, jenis penelitiannya kualitatif (qualitative research), secara eksklusif (content analysis) dan teknik pengumpulan data melalui dokumentasi. Hasil dari penelitian penulis yaitu pada proses persidangan majelis hakim Mahkamah Syar’iyah Kota Banda Aceh menolak gugatan tergugat disebabkan tidak terbukti pihak tergugat melakukan wanprestasi dan secara tegas menolak seluruh tuntutan pihak penggugat atas perjanjian pembiayaan murabahah bi al-wakalah telah dilaksanakan sepenuhnya oleh pihak tergugat, dan seluruhnya sesuai dengan kesepakatan yang dimuat dalam perjanjian tersebut. Namun dalam putusan ini ada bagian yang tidak terungkap terutama pada alasan pihak tergugat yang secara langsung telah menyalurkan pembiayaan tahap kedua kepada pihak developer seharusnya berdasarkan akad perjanjian pencairan tahap kedua tersebut tetap harus dilakukan pihak nasabah debitur. Dengan demikian putusan yang dibuat majelis hakim ini masih perlu ditinjau ulang terutama mengungkap fakta terjadinya pentransferan dana tahap kedua langsung kepada pihak developer sebelum rumah yang diorder nasabah debitur selesai dibangun dan diserahkan kepada pihak penggugat.
KONTEKSTUALISASI MAKNA SENIF RIQAB DALAM PERSPEKTIF ULAMA KONTEMPORER
Authors: Musa Armiadi 
Published: June 2024

Sebagian Ulama Kontemporer seperti Mahmud Syaltut, memandang bahwa perbudakan yang dimaksudkan dalam al-Quran surat at-Taubah ayat 60, sudah tidak ditemukan lagi faktanya di dunia sekarang ini, karena itu penafsiran riqab perlu diperluas tidak hanya menyangkut pembebasan budak tetapi juga upaya membebaskan negara-negara yang masih dikuasai negara lain yang bertindak dzalim baik secara politik, ekonomi, maupun ideologis. Negara-negara seperti ini masuk dalam cengkeraman perbudakan dan mengekang kebebasan warganya yang berdampak pada aspek kemanusiaan secara global lebih mengerikan daripada sekedar perbudakan hamba sahaya. Dengan demikian terma riqab tidak lagi harus dipahami secara literal, karena riqab dalam konteks budak sudah tidak relevan lagi. Namun jika ‘illat yang ada dalam pemikiran ulama dipertimbangkan dan disesuaikan dengan keadaan sekarang, maka riqab dikontekstualisasikan pemaknaanya agar sesuai dengan kondisi sekarang. Menurut al-Qurthubi dan Muhammad Rasyid Rida, terkait membebaskan bangsa yang terjajah dengan zakat, Rasyid Rida berpendapat dibolehkan sekiranya tiada lagi keperluan untuk menebus kemerdekaan hamba sahaya secara individu. Beliau mengemukakan bahwa bagian senif riqab boleh dipergunakan untuk membantu sesuatu bangsa yang ingin melepaskan dirinya dari penjajahan, apabila tidak ada sasaran membebaskan, perorangan. Adapun Yusuf Qardhawi cenderung menyatakan bahwa tidak perlu memperluas pengertian kalimat yang madlul aslinya tidak menunjukkan demikian, sebab menolong bangsa yang sedang memperjuangkan kemerdekaannya, merupakan tanggungjawab Bersama, bukan membebankan bantuannya pada dari senif zakat. Riqab pada konteks kekinian, khususnya di Indonesia dan Malaysia dapat disamakan dengan human trafficking atau perdagangan orang., sehingga direkomendasikan kepada Lembaga-lembaga keamilan dapat membebaskan mereka dari perbudakan modern.
Analisis Learning Management System-based Coursework terhadap Nilai Mahasiswa melalui Analytics Dashboard
Authors: Meliana Selly , Taufik Rahman , Handono Aryo 
Published: June 2024

The Pandemic era caused many changes, including teaching and content delivery strategies. Students should study from home, whilst lectures are required to quickly adapt how to teach online. Less engagement in online classes forced lectures to prepare additional coursework, such as pretests, posttests, and exercises, for students with the expectation of being able to track their students’ learning progress. This paper will concentrate on whether the Learning Management System (LMS)-based coursework, prepared by the lecturer, is worth the effort and positively correlates to students’ learning outcomes. Spearman correlation will be utilized in this study based on dataset characteristics. We implement correlation results from exploratory data analysis into the dashboard development. The dashboard displays a visual of the correlation between coursework. The visualization can help the lecturer decide which coursework is necessary and which should be removed, reduced, preserved, or extended.
Pengaruh Bauran Pemasaran Digital sebagai Pengembangan Strategi Pemasaran Menggunakan Model SOSTAC untuk Mendukung Keputusan Pemilihan Kampus
Authors: Hariyanti Novi Tri , Wirapraja Alexander 
Published: June 2024

Persaingan organisasi tidak hanya terjadi pada perusahaan bisnis saja namun hingga pada tingkat perguruan tinggi. Semakin banyaknya ekspansi dari kampus negeri maupun swasta menyebabkan setiap kampus perlu melakukan pendekatan yang kreatif dan inovatif. Kampus X sebagai salah satu perguruan tinggi swasta di Jawa Timur juga terdorong untuk segera bertindak menyesuaikan dengan kondisi iklim persaingan yang semakin ketat. Pada penelitian ini akan diteliti mengenai pengaruh dari bauran pemasaran dengan menggunakan model Situation Analysis, Objectives, Strategy, Tactics, Action dan Control (SOSTAC) sebagai upaya untuk mendukung pengambilan keputusan penentuan strategi digital yang tepat. Penelitian ini menggunakan model kualitatif deskriptif dengan melakukan wawancara kepada informan. nforman yang digunakan adalah staf penerimaan mahasiswa baru pada kampus X. Hasil yang didapatkan adalah dengan model SOSTAC kampus X dapat mengetahui kelebihan dan kekurangan yang mereka miliki dan dapat melakukan peningkatan terhadap strategi yang saat ini telah berjalan agar menjadi lebih baik lagi dan sesuai dengan tujuan yang ingin dicapai.

Organizational competition occurs not only in business companies but also at the university level. The increasing number of expansions from public and private campuses means that each campus needs to take a creative and innovative approach. Campus X, one of private universities in East Java, is also encouraged to act immediately to adapt to increasingly tight competitive conditions. In this research, the influence of the marketing mix was examined using the SOSTAC model as an effort to support decision-making in determining the right digital strategy. This research uses a descriptive qualitative model by conducting interviews with informants, where the informants used were new student admissions staff at campus X. The results are, with the SOSTAC model, Campus X can find the strengths and weaknesses they have and improve the current strategy so that it becomes even better and in line with the goals to be achieved.
Implementasi Metode MOORA dalam Sistem Pendukung Keputusan bagi Calon Penerima Beasiswa Program Indonesia Pintar di SMPN Satu Atap Nununamat
Authors: Nahak Eviana , Tedy Frengky , Siki Yovinia C. Hoar , Ngaga Emerensiana , Jando Emanuel , Mau Sisilia Daeng Bakka 
Published: June 2024

Beasiswa Program Indonesia Pintar adalah pemberian beasiswa berupa uang tunai yang bertujuan membantu meringankan biaya pendidikan bagi siswa yang kurang mampu agar tetap melanjutkan pendidikannya. SMPN Satu Atap Nununamat adalah salah satu sekolah yang memberikan beasiswa Program Indonesia Pintar (PIP) setiap tahun ajaran baru, di mana saat proses pemberian penerimaan beasiswa PIP sering memakan waktu yang lama dan sering tidak tepat sasaran karena banyak siswa yang memiliki kesamaan kriteria. Oleh karena itu, pada penelitian ini dibangun sebuah Sistem Pendukung Keputusan (SPK) yang dapat merekomendasikan calon penerima beasiswa PIP yang menggunakan 5 kriteria yaitu: nilai rapor, presensi kehadiran, pekerjaan orang tua, penghasilan orang tua dan jumlah tanggungan. Data kriteria di-input-kan pada aplikasi SPK penerima beasiswa PIP dan pengolahan data menggunakan metode Multi Objective Optimization On The Basis Of Ratio Analysis (MOORA). Hasil yang didapatkan dari penelitian ini adalah sistem berbasis website yang dapat membantu memberikan kemudahan bagi staf tata usaha di Sekolah Menengah Pertama Negeri Satu Atap Nununamat dalam proses seleksi menentukan penerima beasiswa PIP agar tidak salah sasaran serta memberikan keputusan dengan cepat dan tepat.

Indonesia Smart Scholarship Program is a scholarship in the form of cash that aims to help offset the cost of education for underprivileged students to continue their education. Nununamat One Roof Middle School is one of the schools that provides Smart Indonesia Program (PIP) scholarships every new school year, where the process of granting PIP scholarships often takes a long time and is often not on target because many students have the same criteria. Therefore, in this study, a Decision Support System (DSS) was built that can recommend potential PIP scholarship recipients using 5 criteria, namely: report card scores, attendance presence, parents' occupation, parents' income, and number of dependents. Criteria data is inputted into the SPK application for PIP scholarship recipients and data processing uses the Multi Objective Optimization On The Basis Of Ratio Analysis (MOORA) method. The results obtained from this study are a website-based system that can help make it easy for administrative staff at the Nununamat Satu Atap Public Junior High School in the selection process to determine PIP scholarship recipients so as not to misdirect and make decisions quickly and accurately.