bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tropical Biases in CMIP5 Multimodel Ensemble: The Excessive Equatorial Pacific Cold Tongue and Double ITCZ Problems*
Authors: Li Gen , Xie Shang-Ping 
Published: November 2013

Abstract
Errors of coupled general circulation models (CGCMs) limit their utility for climate prediction and projection. Origins of and feedback for tropical biases are investigated in the historical climate simulations of 18 CGCMs from phase 5 of the Coupled Model Intercomparison Project (CMIP5), together with the available Atmospheric Model Intercomparison Project (AMIP) simulations. Based on an intermodel empirical orthogonal function (EOF) analysis of tropical Pacific precipitation, the excessive equatorial Pacific cold tongue and double intertropical convergence zone (ITCZ) stand out as the most prominent errors of the current generation of CGCMs. The comparison of CMIP–AMIP pairs enables us to identify whether a given type of errors originates from atmospheric models. The equatorial Pacific cold tongue bias is associated with deficient precipitation and surface easterly wind biases in the western half of the basin in CGCMs, but these errors are absent in atmosphere-only models, indicating that the errors arise from the interaction with the ocean via Bjerknes feedback. For the double ITCZ problem, excessive precipitation south of the equator correlates well with excessive downward solar radiation in the Southern Hemisphere (SH) midlatitudes, an error traced back to atmospheric model simulations of cloud during austral spring and summer. This extratropical forcing of the ITCZ displacements is mediated by tropical ocean–atmosphere interaction and is consistent with recent studies of ocean–atmospheric energy transport balance.
The Doppler Aerosol Wind (DAWN) Airborne, Wind-Profiling Coherent-Detection Lidar System: Overview and Preliminary Flight Results
Authors: Kavaya Michael J. , Beyon Jeffrey Y. , Koch Grady J. , Petros Mulugeta , Petzar Paul J. , Singh Upendra N. , Trieu Bo C. , Yu Jirong 
Published: November 2013

Abstract
The first airborne wind measurements of a pulsed, 2-μm solid-state, high-energy, wind-profiling lidar system for airborne measurements are presented. The laser pulse energy is the highest to date in an eye-safe airborne wind lidar system. This energy, the 10-Hz laser pulse rate, the 15-cm receiver diameter, and dual-balanced coherent detection together have the potential to provide much-improved lidar sensitivity to low aerosol backscatter levels compared to earlier airborne-pulsed coherent lidar wind systems. Problems with a laser-burned telescope secondary mirror prevented a full demonstration of the lidar’s capability, but the hardware, algorithms, and software were nevertheless all validated. A lidar description, relevant theory, and preliminary results of flight measurements are presented.
Climate-Sensitive Decisions and Time Frames: A Cross-Sectoral Analysis of Information Pathways in the Carolinas
Authors: Lackstrom Kirsten , Kettle Nathan P. , Haywood Benjamin , Dow Kirstin 
Published: November 2013

Abstract
This paper analyzes the information dissemination pathways that support climate-sensitive decisions in North and South Carolina. The study draws from over 100 online questionnaires and follow-up interviews with leaders in the forestry, natural resources management, planning and preparedness, tourism and recreation, and water supply management sectors. Participants represented subregions within each state, different types of organizations, and organizations working at different geographic scales. The cross-sector comparison demonstrates diverse information uses across multiple time horizons and a wide range of sector-specific needs and factors that influence how and where decision makers obtain climate information. It builds upon previous research regarding climate decision making by providing a comprehensive view of the patterns of information exchange within a given region. Although all sectors draw from a common pool of federal agencies for historical and current climate data, participants consider sector-specific and local sources to be their key climate information providers. Information obtained through these sources is more likely to be trusted, accessible, and relevant for decision making. Furthermore, information sharing is largely facilitated via subregional networks, and accessing relationships with colleagues and local agency personnel is a critical component of this process. This study provides a more nuanced understanding of how climate information use varies across sectors and time frames and the decentralized nature of existing networks. These findings have important implications for future efforts to provide climate decision support to state- and local-level decision makers and highlight the need for networks and processes that meet diverse regional and sector concerns and contexts.
On coupled fixed point results in asymmetric G-metric spaces
Authors: Agarwal Ravi P , Kadelburg Zoran , Radenović Stojan 
Published: November 2013

Abstract
Using a combination of techniques introduced by Jleli and Samet (Fixed Point Theory Appl. 2012:210, 2012) and Samet et al. (Int. J. Anal. 2013:917158, 2013) on the one hand, and by Kadelburg et al. (Bull. Math. Anal. Appl. 4:51-63, 2012) on the other hand, we show that several coupled fixed point results in (ordered) G-metric spaces obtained recently are simple consequences of the respective standard (ordered) metric results. The technique can be applied both in symmetric and asymmetric cases. Moreover, we show by an example that the results thus obtained are usually stronger than those presented in the literature.

MSC:47H10, 54H25.
The increase of vasomotor tone avoids the ability of the dynamic preload indicators to estimate fluid responsiveness
Authors: Bouchacourt Juan P , Riva Juan A , Grignola Juan C 
Published: November 2013

Abstract

Background
The use of vasoconstrictor can affect the dynamic indices to predict fluid responsiveness. We investigate the effects of an increase of vascular tone on dynamic variables of fluid responsiveness in a rabbit model of hemorrhage, and to examine the ability of the arterial pressure surrogates dynamic indices to track systolic volume variation (SVV) during hypovolemia under increased vasomotor tone.

Methods
Eighteen anesthetized and mechanically ventilated rabbits were studied during normovolemia (BL) and after blood progressive removal (15 mL/kg, BW). Other two sets of data were obtained during PHE infusion with normovolemia (BL + PHE) and during hypovolemia (BW + PHE). We measured central venous and left ventricular (LV) pressures and infra diaphragmatic aortic blood flow (AoF) and pressure. Pulse pressure variation (PPV), systolic pressure variation (SPV) and SVV were estimated manually by the variation of beat-to-beat PP, SP and SV, respectively. We also calculated PPVapnea as 100 × (PPmax-PPmin)/PP during apnea. The vasomotor tone was estimated by total peripheral resistance (TPR = mean aortic pressure/mean AoF), dynamic arterial elastance (Eadyn = PPV/SVV) and arterial compliance (C = SV/PP). We assessed LV preload by LV end-diastolic pressure (LVEDP). We compared the trending abilities between SVV and pressure surrogate indices using four-quadrant plots and polar plots.

Results
Baseline PPV, SPV, PPVapnea, and SVV increased significantly during hemorrhage, with a decrease of AoF (P < 0.05). PHE induced significant TPR and Eadyn increase and C decrease in bled animals, and a further decrease in AoF with a significant decrease of all dynamic indices. There was a significant correlation between SVV and PPV, PPVapnea and SPV in normal vasomotor tone (r2 ≥ 0.5). The concordance rate was 91%, 95% and 76% between SVV and PPV, PPVapnea and SPV, respectively, in accordance with the polar plot analysis. During PHE infusion, there was no correlation between SVV and its surrogates, and both four-quadrant plot and polar plot showed poor trending.

Conclusion
In this animal model of hemorrhage and increased vasomotor tone induced by phenylephrine the ability of dynamic indices to predict fluid responsiveness seems to be impaired, masking the true fluid loss. Moreover, the arterial pressure surrogates have not the reliable trending ability against SVV.
Increased leptin and A-FABP levels in relapsing and progressive forms of MS
Authors: Messina Silvia , Vargas-Lowy David , Musallam Alexander , Healy Brian C , Kivisakk Pia , Gandhi Roopali , Bove Riley , Gholipour Taha , Khoury Samia , Weiner Howard L , Chitnis Tanuja 
Published: November 2013

Abstract

Background
Leptin and adipocyte-fatty acid binding protein (A-FABP) are produced by white adipose tissue and may play a role in chronic inflammation in Multiple Sclerosis (MS). To assess leptin and A-FABP in relapsing and progressive forms of MS.

Methods
Adipokine levels were measured in untreated adult relapsing-remitting MS (RRMS), secondary progressive MS (SPMS), primary progressive MS (PPMS) and Healthy control (HC). Pediatric-onset MS (POMS) and pediatric healthy controls (PHC) were also assessed. Leptin and A-FABP levels were measured in serum by ELISA. Groups were compared using linear mixed-effects model.

Results
Excluding two patients with Body Mass Index (BMI) > 50, a significant difference in leptin level was found between RRMS and HC controlling for age (p = 0.007), SPMS and HC controlling for age alone (p = 0.002), or age and BMI (p = 0.007). A-FABP levels were higher in SPMS than HC (p = 0.007), controlling for age and BMI. Differences in A-FABP levels between POMS and PHC was observed after controlling for age (p = 0.019), but not when BMI was added to the model (p = 0.081).

Conclusion
Leptin and A-FABP levels are highest in SPMS compared to HC, suggesting a role in pathogenesis of this disease subtype. A-FABP levels are increased in POMS patients and may play a role in the early stages of disease.
Novel angiotensin I-converting enzyme inhibitory peptides derived from an edible mushroom, Pleurotus cystidiosus O.K. Miller identified by LC-MS/MS
Authors: Lau Ching Ching , Abdullah Noorlidah , Shuib Adawiyah Suriza 
Published: November 2013

Abstract

Background
Angiotensin I-converting enzyme (ACE) inhibitors have been reported to reduce mortality in patients with hypertension. Compared to chemosynthetic drugs, ACE inhibitors derived from natural sources such as food proteins are believed to be safer for consumption and to have fewer adverse effects. Some edible mushrooms have been reported to significantly reduce blood pressure after oral administration. In addition, mushrooms are known to be rich in protein content. This makes them a potential source of ACE inhibitory peptides. Hence, the objective of the current study was to isolate and characterise ACE inhibitory peptides from an edible mushroom, Pleurotus cystidiosus.

Methods
ACE inhibitory proteins were isolated from P. cystidiosus based on the bioassay guided purification steps, i.e. ammonium sulphate precipitation, reverse phase high performance liquid chromatography and size exclusion chromatography. Active fraction was then analysed by LC-MS/MS and potential ACE inhibitory peptides identified were chemically synthesized. Effect of in vitro gastrointestinal digestions on the ACE inhibitory activity of the peptides and their inhibition patterns were evaluated.

Results
Two potential ACE inhibitory peptides, AHEPVK and GPSMR were identified from P. cystidiosus with molecular masses of 679.53 and 546.36 Da, respectively. Both peptides exhibited potentially high ACE inhibitory activity with IC50 values of 62.8 and 277.5 μM, respectively. SEC chromatograms and BIOPEP analysis of these peptides revealed that the peptide sequence of the hexapeptide, AHEPVK, was stable throughout gastrointestinal digestion. The pentapeptide, GPSMR, was hydrolysed after digestion and it was predicted to release a dipeptide ACE inhibitor, GP, from its precursor. The Lineweaver-Burk plot of AHEPVK showed that this potent and stable ACE inhibitor has a competitive inhibitory effect against ACE.

Conclusion
The present study indicated that the peptides from P. cystidiosus could be potential ACE inhibitors. Although these peptides had lower ACE inhibitory activity compared to commercial antihypertensive drugs, they are derived from mushroom which could be easily obtained and should have no side effects. Further in vivo studies can be carried out to reveal the clear mechanism of ACE inhibition by these peptides.
The anti-erbB3 antibody MM-121/SAR256212 in combination with trastuzumab exerts potent antitumor activity against trastuzumab-resistant breast cancer cells
Authors: Huang Jingcao , Wang Shuiliang , Lyu Hui , Cai Bo , Yang XiaoHe , Wang Jianxiang , Liu Bolin 
Published: November 2013

Abstract

Background
Elevated expression of erbB3 receptor has been reported to induce resistance to therapeutic agents, including trastuzumab in erbB2-overexpressing breast cancer. Our recent studies indicate that erbB3 interacts with both erbB2 and IGF-1 receptor to form a heterotrimeric complex in trastuzumab-resistant breast cancer cells. Herein, we investigate the antitumor activity of MM-121/SAR256212, a fully human anti-erbB3 antibody (Ab), against two erbB2-overexpressing breast cancer cell lines resistant to trastuzumab.

Methods
MTS-based proliferation assays were used to determine cell viability upon treatment of trastuzumab and/or MM-121/SAR256212. Cell cycle progression was examined by flow cytometric analysis. Western blot analyses were performed to determine the expression and activation of proteins. Tumor xenografts were established by inoculation of the trastuzumab-resistant BT474-HR20 cells into nude mice. The tumor-bearing mice were treated with trastuzumab and/or MM-121/SAR256212 via i.p injection to determine the Abs’ antitumor activity. Immunohistochemical analyses were carried out to study the Abs’ inhibitory effects on tumor cell proliferation and induction of apoptosis in vivo.

Results
MM-121 significantly enhanced trastuzumab-induced growth inhibition in two sensitive and two resistant breast cancer cell lines. MM-121 in combination with trastuzumab resulted in a dramatic reduction of phosphorylated erbB3 (P-erbB3) and Akt (P-Akt) in the in vitro studies. MM-121 combined with trastuzumab did not induce apoptosis in the trastuzumab-resistant cell lines under our cell culture condition, rather induced cell cycle G1 arrest mainly associated with the upregulation of p27kip1. Interestingly, in the tumor xenograft model established from the trastuzumab-resistant cells, MM-121 in combination with trastuzumab as compared to either agent alone dramatically inhibited tumor growth correlated with a significant reduction of Ki67 staining and increase of cleaved caspase-3 in the tumor tissues.

Conclusions
The combination of MM-121 and trastuzumab not only inhibits erbB2-overexpressing breast cancer cell proliferation, but also promotes the otherwise trastuzumab-resistant cells undergoing apoptosis in an in vivo xenografts model. Thus, MM-121 exhibits potent antitumor activity when combined with trastuzumab under the studied conditions. Our data suggest that further studies regarding the suitability of MM-121 for treatment of breast cancer patients whose tumors overexpress erbB2 and become resistant to trastuzumab may be warranted.
The patient experience with fatigue and content validity of a measure to assess fatigue severity: qualitative research in patients with ankylosing spondylitis (AS)
Authors: Naegeli April N , Flood Emuella , Tucker Jennifer , Devlen Jennifer , Edson-Heredia Emily 
Published: November 2013

Abstract

Background
Ankylosing spondylitis (AS) is an autoimmune disorder characterized by inflammation of the spine and large joints. Fatigue is a common symptom that many AS patients find significantly impacts their health-related quality of life. The Worst Fatigue – Numeric Rating Scale (WF-NRS) assesses the severity of this symptom during the previous 24-hour period. The objective of this study was to perform qualitative research to support the development and content validity of the WF-NRS.

Methods
Patients with AS were recruited from clinical sites in the U.S. for a qualitative study which first entailed concept elicitation interviews to gain understanding of the patients’ experience with AS and fatigue. Subsequently, cognitive debriefing interviews were undertaken to assess the understandability, clarity, and appropriateness from the patient’s perspective, of the content of a measure of fatigue severity.

Results
Thirteen patients with AS participated in concept elicitation interviews and cognitive debriefing of the Brief Fatigue Inventory (BFI) fatigue severity subscale. The WF-NRS was developed from the worst fatigue item of the BFI as patients generally reported it to be understandable and covered an important concept, the completion instructions were modified, but the response scale remained as it was familiar and readily completed, and the recall period was appropriate.

Conclusions
Patient responses resulted in the development of and supported the content validity of the WF-NRS. Further quantitative evaluation of the WF-NRS is warranted in order to assess its psychometric properties and confirm its usefulness as a clinical trial tool.
Adult self-reported and objectively monitored physical activity and sedentary behavior: NHANES 2005–2006
Authors: Schuna John M , Johnson William D , Tudor-Locke Catrine 
Published: November 2013

Abstract

Background
It remains unclear what people are attempting to communicate, in terms of objectively monitored behavior, when describing their physical activity and sedentary behavior through self-report. The purpose of this study was to examine various objectively monitored accelerometer variables (e.g., moderate-to-vigorous physical activity [MVPA], steps/day, sedentary time, etc.) across categories of self-reported MVPA (< 150 vs. ≥ 150 minutes/week), usual occupational/domestic activity (UODA; “mostly sitting” vs. “stand, walk, lift, or carry”), and leisure-time sedentary behavior (LTSB; ≥ 3 vs. < 3 hours/day) in a nationally representative sample of U.S. adults (≥ 20 years).

Methods
This is a secondary analysis of 3,725 participants from the 2005–2006 National Health and Nutrition Examination Survey (NHANES) who provided relevant questionnaire responses and ≥ 1 day of valid accelerometer data. Descriptive statistics were computed for various objectively monitored accelerometer variables across categories of self-reported MVPA, UODA, and LTSB. Pairwise comparisons were conducted to examine differences in objectively monitored behavior between categories of self-reported MVPA, UODA, and LTSB.

Results
On average, adults reporting compliance with physical activity guidelines (≥ 150 minutes/week of MVPA) accumulated more objectively measured physical activity and similar amounts of sedentary time relative to those reporting not achieving guidelines. Adults reporting their daily UODA as “mostly sitting” or accruing ≥ 3 hours/day of LTSB accumulated less objectively monitored physical activity and more sedentary time than those who described their UODA as “stand, walk, lift, or carry” or accrued < 3 hours/day of LTSB. The most active cross-classified category (7,935 steps/day; ≥ 150 minutes/week of self-reported MVPA, “stand, walk, lift, or carry” UODA, and < 3 hours/day of LTSB) accumulated more than twice as many daily steps as the least active cross-classified category (3,532 steps/day; < 150 minutes/week of self-reported MVPA, “mostly sitting” UODA, and ≥ 3 hours/day of LTSB).

Conclusions
A number of objectively monitored physical activity indicators varied significantly between self-reported MVPA, UODA, and LTSB categories, while objectively monitored sedentary time only varied between UODA and LTSB categories. Cross-classifications of self-reported MVPA, UODA, and LTSB responses depict a greater range of physical activity than viewing dichotomous responses for these variables one-at-a-time.
Association of autoantibodies against the M2-muscarinic receptor with perinatal outcomes in women with severe preeclampsia
Authors: Li Yanfang , Ma Guiling , Zhang Zhiyong , Yue Yin , Yuan Yuting , Wang Yidan , Miao Guobin , Zhang Lin 
Published: November 2013

Abstract

Background
The goal of this study was to test the hypothesis that autoantibodies against M2-muscarinic acetylcholine receptor (M2-AAB) are associated with severe preeclampsia and increased risk of adverse perinatal outcomes.

Methods
We conducted a case–control study comparing 60 women with severe preeclampsia to 60 women with normal pregnancy and 60 non-pregnant controls. A peptide, corresponding to amino acid sequences of the second extracellular loops of the M2 receptor, was synthesized as antigen to test for the presence of autoantibodies, using an enzyme-linked immunosorbent assay. The frequency and titer of M2-AAB were compared in the 3 groups. The risk of adverse perinatal outcomes among women with severe preeclampsia in the presence of M2-AAB was estimated.

Results
M2-AAB were positive in 31.7% (19/60) of patients with severe preeclampsia, in 10.0% (6/60) (p = 0.006) of normal pregnant women and in 8.3% (5/60) (p = 0.002) of non-pregnant controls. The presence of M2-AAB was associated with increased risk of adverse pregnancy complications (OR, 3.6; 95%CI, 1.0-12.6; p = 0.048), fetal growth restriction (OR, 6.8; 95% CI, 2.0-23.0; p = 0.002), fetal distress (OR, 6.7; 95% CI, 1.7-26.6; p = 0.007), low Apgar score (OR, 5.3; 95% CI, 1.4-20.7; p = 0.017), and perinatal death (OR, 4.3; 95% CI, 1.0-17.6; p = 0.044) among women with severe preeclampsia.

Conclusions
This study demonstrates, for the first time, an increase in M2-AAB in patients with severe preeclampsia. Women with severe preeclampsia who are M2-AAB positive are at increased risk for neonatal mortality and morbidity. We posit that M2-AAB may be involved in the pathogenesis of severe preeclampsia.
Re-assessment of selected Baby-Friendly maternity facilities in Accra, Ghana
Authors: Nii Okai Aryeetey Richmond , Antwi Comfort Liousa 
Published: November 2013

Abstract

Background
The Baby-Friendly Hospital Initiative (BFHI) has been implemented in Ghana since 1995. At the end of 2011, about 325 maternity facilities in Ghana had been designated Baby Friendly. However, none had been re-assessed for adherence to the Ten Steps to successful breastfeeding (Ten Steps). The current study re-assessed six maternity facilities in Accra for adherence to the Ten Steps and the International Code of Marketing of breast milk substitutes (the Code).

Methods
Three independent assessors performed the re-assessment using the revised WHO/UNICEF external re-assessment tool (ERT) between April and June, 2011. All sections of the ERT were implemented, except for the HIV/infant feeding section. Assessors interviewed 90 clinical staff of the facilities, 60 pregnant women, and 150 women who had given birth and waiting to be discharged from the hospital. Additionally, observations were completed on neonate feeding and compliance with the Code. Data was analyzed to assess adherence to the Ten Steps and the Code.

Results
In 2010, the six facilities recorded a total of 26,339 deliveries. At discharge, the weighted exclusive breastfeeding rate was 93.8%. None of the facilities adhered completely to the Ten Steps. Overall, the rate of adherence to the Ten Steps was 42% (range = 30 - 70%). No facility met the criteria for Steps One and Two. Only Step Seven was adhered to by all facilities. Overall compliance with the Code was about 54%. Trained staff attrition, high client-staff ratios, inadequate in-service training for new staff, and inadequate support for regional and national program monitoring were identified as barriers to adherence.

Conclusion
Poor adherence to Baby-Friendly practices in designated BFHI facilities was observed in urban Accra. Renewed efforts to support monitoring of designated facilities is recommended.