bluuebunny/crossref_metadata_2025_split huggingface dataset:
Incidence of 17p deletions and TP53 mutation in myelodysplastic syndrome and acute myeloid leukemia with 5q deletion
Authors: Sebaa Amel , Ades Lionel , Baran‐Marzack Fanny , Mozziconacci Marie‐Joelle , Penther Dominique , Dobbelstein Sophie , Stamatoullas Aspasia , Récher Christian , Prebet Thomas , Moulessehoul Soraya , Fenaux Pierre , Eclache Virginie 
Published: August 2012

AbstractTP53 mutations are frequent in myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML) with complex karyotype that include del(5q) and are often associated with deletion of 17p. They have also recently been observed in MDS with isolated del(5q). We assessed the incidence of 17p deletion detected by fluorescence in situ hybridization (FISH) and of TP53 mutations detected by direct sequencing and their correlation and prognostic value in 26 MDS and 17 AML with del(5q). In the 20 cases with isolated del(5q) or one additional abnormality, no 17p deletion was found and 3 of the 18 cases analyzed (17%) had TP53 mutation. In the 23 patients with complex karyotype, 17p deletion was suspected by conventional cytogenetics in 15 cases and confirmed by FISH in 10 of them, while TP53 mutation was found in 8 of the 15 patients tested (53%), only five of whom had 17p deletion. In the whole patient series, TP53 mutations were associated with shorter survival (P = 0.07). We confirm the existence of TP53 mutations in 17% of MDS with isolated del(5q). In patients with del(5q) and complex karyotype, FISH and direct sequencing are complementary techniques to analyze TP53 abnormalities. Our findings also suggest that sequencing of the TP53 gene should be included in the study of patients with del(5q) as a single abnormality or in complex karyotype before lenalidomide treatment. © 2012 Wiley Periodicals, Inc.
TWO‐LEVEL RESAMPLING AS A NOVEL METHOD FOR THE CALCULATION OF THE EXPECTED VALUE OF SAMPLE INFORMATION IN ECONOMIC TRIALS
Authors: Sadatsafavi Mohsen , Marra Carlo , Bryan Stirling 
Published: August 2012

ABSTRACTIt has already been pointed out that the bootstrap can be used to calculate the expected value of perfect information (EVPI) when individual‐level data from a randomized controlled trial (RCT) is at hand. However, as mentioned by others, it is not clear if and how such a method can be extended to calculate the expected value of sample information (EVSI). In this article, we provide a nonparametric definition for EVPI and EVSI, which is based on considering the entire population distribution as the uncertain entity for which the current RCT provides partial information. This enables a theoretical justification for using the bootstrap for EVPI calculation and allows us to propose a two‐level resampling method for EVSI calculation. What is considered as the sampling unit in this algorithm can range from the individual level net benefits to the full panel of the RCT data for an individual, enabling the analyst to decide on a trade‐off between computational efficiency and comprehensiveness in value of information analysis. As such, we argue that this method, in addition to being consistent with the popular bootstrap method of RCT‐based economic evaluations, is a flexible approach for EVPI and EVSI calculations. Copyright © 2012 John Wiley & Sons, Ltd.
DETERMINANTS OF ROAD TRAFFIC CRASH FATALITIES ACROSS INDIAN STATES
Authors: Grimm Michael , Treibich Carole 
Published: August 2012

SUMMARYThis article explores the determinants of road traffic crash fatalities in India. In addition to income, the analysis considers the sociodemographic population structure, motorization levels, road and health infrastructure and road rule enforcement as potential factors.An original panel data set covering 25 Indian states is analyzed using multivariate regression analysis. Time and state fixed‐effects account for unobserved heterogeneity across states and time.The rising motorization, urbanization and accompanying increase in the share of vulnerable road users, that is, pedestrians and two‐wheelers, are the major drivers of road traffic crash fatalities in India. Among vulnerable road users, women form a particularly high‐risk group. Higher expenditure per police officer is associated with a lower fatality rate.The results suggest that India should focus, in particular, on road infrastructure investments that allow the separation of vulnerable from other road users on improved road rule enforcement and should pay special attention to vulnerable female road users. Copyright © 2012 John Wiley & Sons, Ltd.
Winter wave climate, storms and regional cycles: the SW Spanish Atlantic coast
Authors: Rangel‐Buitrago N. , Anfuso G. 
Published: August 2012

ABSTRACTClimatic change‐related impacts on coastal areas became an important issue in past decades and nowadays threaten many human settlements and activities. Coastal hazards are linked to flooding and erosion processes associated with sea level rise and the increased strength of hurricanes, cyclones and storms. The main aim of this work is the characterization of coastal storms in Cadiz (SW Spain) and the determination of their recurrence intervals and relationships with several regional cycles. Storm characterization was carried out using the Storm Power Index (Dolan and Davis, 1992) and five classes were obtained, from class I (weak events) to V (extreme events). Storm occurrence probability was 96% for class I (i.e. almost one event per year) to 3% for class V. The return period for class V was 25 years and ranged from 6 to 8 years for classes III and IV storms, e.g. significant and severe events. Classes I and II showed a period of recurrence ranging from 1 to 3 years. Stormy winter seasons were 2009/10 (12 events), 1995/6 and 2002/3 (with 10 events each) and 1993/4 (8 events). Approximately 40% of the change in monthly wave data and storminess indices was related to several teleconnection patterns, the most important drivers of change being the Arctic Oscillation (AO), 21.45%, and the North Atlantic Oscillation (NAO), 19.65%. It is interesting to note that a great number of storms, larger storm duration and higher values of Storm Power Index were only observed when neutral to strong negative NAO and AO phases occurred at the same time (89 storms and 3355 h) and/or when there was an abrupt change of NAO and AO phases, i.e. they moved from a positive to negative phase without passing through a neutral phase. The results obtained in this work have wider applications for ocean and coastal management. It is suggested that methodology used can be easily applied in different areas where wave buoy data are available. In the same way, information obtained with this kind of work constitutes the first step in the development of coastal protection plans to preserve socio‐economic activities from the impact of severe storm events.
Regional climate model application at subgrid scale on Indian winter monsoon over the western Himalayas
Authors: Dimri A. P. , Niyogi D. 
Published: August 2012

ABSTRACTThe western Himalayas (WH) is characterized by heterogeneous land surface characteristics and topography. During winter (December, January, and February—DJF), eastward moving low‐pressure synoptic weather systems, called Western Disturbances (WDs) in Indian parlance, cause the majority of the precipitation mostly in the form of snow. The interplay between land surface/topography and WDs greatly controls precipitation distribution over the region. This study seeks to evaluate this using a mosaic‐type parameterization of subgrid‐scale topography and land use (sub‐BATS) for regional climate simulation with a regional climate model (RegCM3). The model coarse grid cell size in the control simulation is 60 km while the subgrid cell size is 10 km. This study compares two 22 year simulations (1980–2001) during winter (DJF). The first simulation is without (CONT) and the second is with (SUB) the fine scale subgrid scheme. Representing the fine scale processes using the subgrid scheme SUB experiment simulates reduced precipitation by approximately 2 mm d−1 with comparison to CONT experiment. This estimation of reduced and closer to the corresponding observed precipitation is important for regional water budget over the WH which is primarily governed by topographic and land surface disaggregation. Validation with corresponding observations over similar elevations shows that SUB displays an improvement over CONT experiment. This relevant decrease of precipitation in SUB using disaggregation‐reaggregation methodology for initial model input fields in subBATS scheme is due to better representation of the WH topography. In case of temperature, SUB experiment displays colder bias (∼2–4 °C) than the CONT over the Himalayas. This preliminary finding is important for studying regional water balance, snow melt accumulation in following summer period.
Chemical composition and functional characterisation of commercial pumpkin seed oil
Authors: Procida Giuseppe , Stancher Bruno , Cateni Francesca , Zacchigna Marina 
Published: August 2012

AbstractBACKGROUND: Pumpkin (Cucurbita pepo L.) seed oil is a common product in Slovenia, Hungary and Austria and is considered a preventive agent for various pathologies, particularly prostate diseases. These properties are related to its high content of carotenoids and liposoluble vitamins. In this study the carotenoid (lutein and zeaxanthin), vitamin E (α‐ and γ‐tocopherol) and fatty acid contents of 12 samples of commercial pumpkin seed oil were investigated together with the composition of the volatile fraction resulting from the roasting process.RESULTS: The aromatic profile obtained from the commercial samples was directly related to the intensity of the roasting process of the crushed pumpkin seeds. The roasting temperature played a crucial role in the concentrations of volatile substances originating from Strecker degradation, lipid peroxidation and Maillard reaction.CONCLUSION: The findings suggest that high‐temperature roasting leads to the production of an oil with intense aromatic characteristics, while mild conditions, generally employed to obtain an oil with professed therapeutic characteristics, lead to a product with minor characteristic pumpkin seed oil aroma. The nutraceutical properties of the product are confirmed by the high content of α‐ and γ‐tocopherol and carotenoids. © 2012 Society of Chemical Industry
Influence of some environmental factors on drupe maturation and olive oil composition
Authors: Di Vaio Claudio , Nocerino Sabrina , Paduano Antonello , Sacchi Raffaele 
Published: August 2012

AbstractBACKGROUND: Understanding the relationships between olive cultivars and the cultivation environment as well as optimising cultivation choices can lead to maximum expression of oil production in terms of both quantity and quality. For this purpose, samples of the Ortice olive cultivar grown in two different environments in southern Italy at altitudes of 500 and 50 m above sea level (a.s.l.) were harvested on various dates to monitor drupe maturation and determine the nutritional and chemical characteristics of the oils.RESULTS: Fruits grown at 50 m a.s.l. ripened about 10–15 days earlier than those grown at 500 m a.s.l. The oil obtained at 500 m a.s.l. was characterised by a higher content of total polyphenols and a higher content of oleic and stearic acids.CONCLUSION: The different heat accumulation related to the environment affected drupe development as well as the colouring trend and oil content. Consequently, the growth environment changed the content of Ortice oil fatty acids and polyphenols, while the flavour profile remained fairly stable in both environments, with the cultivar effect prevailing over the environment factor. © 2012 Society of Chemical Industry
Development of a protocol to solidify native and artificial oil bodies for long‐term storage at room temperature
Authors: Chang Ming‐Tsung , Chen Chun‐Ren , Liu Ting‐Hang , Lee Chin‐Pei , Tzen Jason TC 
Published: August 2012

AbstractBACKGROUND: Oil bodies isolated from sesame seeds coalesced to form large oil drops when they were solidified in a drying process commonly used for food products. The aim of this study was to develop a protocol to solidify oil bodies for long‐term storage at room temperature.RESULTS: On the basis of testing several excipients, the coalescence of oil bodies could be effectively prevented when they were combined with mannitol. Sizes of oil bodies appeared similar under a light microscope before and after powderisation in combination with 70% or more mannitol. Artificial oil bodies were successfully generated with sesame oil, phospholipid and recombinant sesame caleosin. Following the developed protocol, native and artificial oil bodies were stably solidified in tablets. Both native and artificial oil bodies dissolved from the tablets remained stable after an accelerated stress test under a condition of 75% humidity at 40 °C for 4 months.CONCLUSION: A protocol was successfully developed for the solidification of native and artificial oil bodies in stable powder and tablet forms. This successful protocol is very likely to expedite the utilisation of artificial oil bodies in their potential applications. © 2012 Society of Chemical Industry
A rapid and simple screening method of Cronobacter spp. in cell suspension and tofu
Authors: Kim Sun Ae , Yu Ji Hyun , Rhee Min Suk 
Published: August 2012

AbstractBACKGROUND: Cronobacter spp. causes illnesses such as neonatal meningitis, sepsis and necrotising enterocolitis that can be fatal in infants and neonates. Thus it is of great concern to the food industry, which requires a simple and miniaturised method for the rapid detection of Cronobacter spp. in food.RESULTS: A simple and rapid method was developed for the quantitative estimation of Cronobacter spp. Cell suspension cultures and tofu inoculated with Cronobacter spp. as well as a mixed culture (Cronobacter spp. and other micro‐organisms) were serially diluted twofold on microtitre plates. Kim and Rhee broth, containing salicin, was added to each well. After 16–18 h of incubation the colour of broth in the wells changed from purple to yellow owing to salicin fermentation by Cronobacter spp. Bacterial counts were predicted by analysing the end points (the last well number in a series of positive wells). The coefficients of determination (r2) between the conventional plating method and the current method had high degrees of correlation (0.954–0.963), suggesting that the latter method offers a reasonable quantification of Cronobacter spp.CONCLUSION: The simple method developed in this study could be used for the efficient and rapid screening of Cronobacter spp. in the food industry. © 2012 Society of Chemical Industry
Oil accumulation in intact olive fruits measured by near infrared spectroscopy–acousto‐optically tunable filter
Authors: Bellincontro Andrea , Caruso Giovanni , Mencarelli Fabio , Gucci Riccardo 
Published: August 2012

AbstractBackgroundA field experiment was conducted to test the reliability of the near infrared spectroscopy (NIR)–acousto‐optically tunable filter (AOTF) method to measure mesocarp oil content in vivo against nuclear magnetic resonance (NMR) determinations using three different olive cultivars at different stages of ripening.ResultsIn the partial least squares model carried out for the cultivar ‘Arbequina’, the coefficient of determination in calibration (R2c) was 0.991, while the coefficient of determination in cross‐validation (R2cv) was 0.979. For the cultivar ‘Frantoio’ the indexes were 0.982 and 0.971, respectively; while for the cultivar ‘Leccino’ R2c was 0.977 and R2cv was 0.965. Finally, for the combined model (sum of the three varieties) these indexes were 0.921 and 0.903, respectively. The residual predictive deviation (RPD) ratio was insufficient for the predictive model of cultivar ‘Leccino’ only (1.98), whereas in the other cases the RPD ratios were completely sufficient, within the estimation range over 2.5–3 (2.61 in the global model, and 4.23 in the cultivar ‘Frantoio’), or in describing a large capacity with values greater than 5, as in the cultivar ‘Arbequina’ (9.58).ConclusionNIR‐AOTF spectroscopy proved to be a novel, rapid and reliable method to monitor the oil accumulation process in intact olive fruits in the field. The innovative approach of coupling NIR and NMR technologies opens up new scenarios for determining the optimal time for harvesting olive trees to obtain maximum oil production.
Northern bobwhite response to Conservation Reserve Program habitat and landscape attributes
Authors: Blank Peter J. 
Published: August 2012

AbstractThe northern bobwhite (Colinus virginianus; hereafter bobwhite) has experienced substantial population declines in recent decades in the United States, and especially in Maryland and Delaware. The United States Department of Agriculture's Conservation Reserve Program (CRP) could provide additional habitat for bobwhites, leading to an increase in bobwhite abundance. I investigated if bobwhite abundance was related to the percent cover of CRP land and landscape attributes in local landscapes on Maryland's Eastern Shore and Delaware. Observers conducted bobwhite point transect surveys at 113 locations during the breeding seasons of 2006–2007, and I calculated landscape metrics for 500‐m radius landscapes centered on each point transect location. Most CRP land in the study landscapes was planted to herbaceous vegetation. Bobwhite abundance was strongly positively associated with percent cover of CRP land in the landscape but was not strongly related to percent cover of agriculture or forest or to landscape patch density. These results suggest that the CRP has created additional habitat for bobwhites in Maryland and Delaware and that landscapes with greater proportions of herbaceous CRP practices support more bobwhites. © 2012 The Wildlife Society.
Skin rejuvenation by microneedle fractional radiofrequency treatment in Asian skin; Clinical and histological analysis
Authors: Seo Kyu Young , Yoon Moon Soo , Kim Dong Hyun , Lee Hee Jung 
Published: August 2012

AbstractBackgroundFor skin rejuvenation, microneedle fractional radiofrequency (RF) is a recently developed minimally invasive method for delivering RF energy directly into the skin using microneedle.ObjectiveWe evaluated efficacy and safety of microneedle fractional RF for skin rejuvenation in Asian skin and also conducted immunohistochemical analysis before and after treatment.Patients and MethodsTwenty‐five females (mean age 54.2, Fitzpatrick skin phototypes III–IV) received three consecutive fractional RF treatment at 4‐week intervals. Outcome assessments included standardized photography, physician's global assessment, patient's satisfaction scores, objective biophysical measurements, and histologic analysis including immunohistochemical staining.ResultsAll patients showed clinical improvement on physician's global assessment and patient's satisfaction scores. Among objective biophysical measurements, improvement in hydration and skin roughness was noticed. Histologic examination revealed marked increase in dermal thickness, dermal collagen content and dermal fibrillin content. Side effects were minimal.LimitationsThe limitations are the small number of patients and lack of long‐term follow‐up.ConclusionMicroneedle fractional RF is a safe and effective skin rejuvenation method in Asians. Lasers Surg. Med. 44: 631–636, 2012. © 2012 Wiley Periodicals, Inc.
Effects of Carbon Nanoreinforcements of Different Shapes on the Mechanical Properties of Epoxy‐Based Nanocomposites
Authors: Alishahi Ehsan , Shadlou Shahin , Doagou‐R Saeed , Ayatollahi Majid Reza 
Published: August 2012

AbstractThe effect of carbon nanoreinforcements of different shapes on the mechanical properties of epoxy‐based composites is studied. It is found that while nanodiamond and fibrous (carbon nanotube and nanofiber) particles provided better tensile properties, platelet (graphene oxide) nanoreinforcements lead to a considerable increase in the fracture toughness of the composites. The trend of the results is explained on the basis of the geometrical characteristics of the reinforcements. The accuracy of several micromechanics‐based criteria for predicting the Young's modulus of composites is investigated for different nanoparticle shapes. The state of dispersion of nanofillers and the fracture surface features of all composites are examined using TEM and SEM. magnified image
Responsive Polyelectrolyte Multilayers Assembled at High Ionic Strength with an Unusual Collapse at Low Ionic Strength
Authors: Irigoyen Joseba , Han Lulu , Llarena Irantzu , Mao Zhenwei , Gao Changyou , Moya Sergio E. 
Published: August 2012

AbstractResponsive polyelectrolyte multilayers (PEMs) of poly(diallyl dimethyl ammonium chloride) (PDADMAC) and poly(styrene sodium sulfonate) (PSS) with thicknesses between 350 and 400 nm for 11 deposited polyelectrolyte layers were fabricated assembling the polyelectrolytes at 3 M NaCl. When the 3 M NaCl bulk solution is replaced by water, the PEMs release water, approximately a 46% of the total mass, and experience a thickness reduction of more than 200 nm. Changes in thickness and water content are fully reversible. The film recovers its original thickness and water content when it is exposed again to a 3 M NaCl solution. A responsive polymer film is achieved with the capability of swelling at high ionic strength and collapsing in water with variations in thickness of hundred of nanometers.
Diaphragm pacing and noninvasive respiratory management of amyotrophic lateral sclerosis/motor neuron disease
Authors: Mahajan Kedar R. , Bach John Robert , Saporito Lou , Perez Nick 
Published: August 2012

AbstractIntroduction: Although it is known that continuous noninvasive ventilation (CNIV) can prolong life in amyotrophic lateral sclerosis/motor neuron disease (ALS/MND), in this study we explore similar claims for diaphragm pacing (DP). Methods: NIV and DP users' vital capacities (VCs) over time and duration of NIV and CNIV dependence were analyzed for 354 non‐DP and 8 DP ALS/MND patients. Results: Patients had a higher rate of monthly VC decline before NIV use (5.1 ± 7.6%) than during NIV use (2.5 ± 3.6%) (P < 0.01, 95% CI 0.84–4.5); the decline for 4 DP users was 3.7–20%. Fifty‐five ALS/MND patients used part‐time NIV for 19.9 ± 27.6 months until tracheostomy/death, whereas 113 others used it for 10.9 ± 10.5 months until CNIV dependence for another 12.8 ± 16.2 months. After placement, 7 DP users were CNIV dependent in 8.0 ± 7.0 months, whereas 6 underwent tracheostomy/died in 18.2 ± 13.7 months. Conclusions: CNIV prolonged the survival of 113 of the 354 non‐DP and 6 DP ALS/MND patients by 12.8 and 10.2 months, respectively. DP provided no benefit on VC or mechanical ventilation–free survival. Muscle Nerve, 2012
Thermoplastic polyurethanes from renewable resources: effect of soft segment chemical structure and molecular weight on morphology and final properties
Authors: Saralegi Ainara , Rueda Lorena , Fernández‐d'Arlas Borja , Mondragon Iñaki , Eceiza Arantxa , Corcuera Mª Angeles 
Published: August 2012

AbstractThe effect of soft segment molecular weight and chemical structure on the morphology and final properties of segmented thermoplastic polyurethanes containing various hard segment contents has been investigated from the viewpoint of the degree of microphase separation. Vegetable oil‐based polyesters and corn sugar‐based chain extender have been used as renewable resources. The synthesis has been carried out in bulk without catalyst using a two‐step polymerization process. Physicochemical, thermal and mechanical properties, and also morphology, have been studied using Fourier transform infrared spectroscopy, differential scanning calorimetry, dynamic mechanical analysis, atomic force microscopy, X‐ray diffraction and mechanical testing. Chemical structure and molecular weight of polyols strongly affect the properties of the synthesized segmented thermoplastic polyurethanes. An increase in soft segment molecular weight leads to an increase of the degree of soft segment crystallinity and microphase separation, thus giving enhanced mechanical properties and higher thermal stability. Copyright © 2012 Society of Chemical Industry