bluuebunny/crossref_metadata_2025_split huggingface dataset:
Office‐based balloon sinus dilation: a prospective, multicenter study of 203 patients
Authors: Karanfilov Boris , Silvers Stacey , Pasha Raza , Sikand Ashley , Shikani Alan , Sillers Michael ,   for the ORIOS2 Study Investigators
Published: November 2012

BackgroundBalloon sinus dilation (BSD) is an increasingly used tool in endoscopic sinus surgery (ESS). The tissue‐sparing nature of the instrumentation allows for properly selected patients to undergo office‐based procedures under local anesthesia.MethodsThis was an Institutional Review Board (IRB)‐approved, prospective, 14‐center trial. Patients (n = 203) requiring ESS for medically refractory chronic sinusitis underwent transnasal BSD treatment in an office setting under local anesthesia. Safety, tolerability, technical success, clinical efficacy (20‐item Sino‐Nasal Outcome Test [SNOT‐20]), and radiographic outcome (Lund‐Mackay [LMK] score) of ESS with BSD in the office setting were assessed. Subjects were followed at 2, 8, and 24 weeks.ResultsA total of 552 sinuses were dilated in 203 patients: 47.6% maxillaries, 45.5% frontals, and 6.9% sphenoids. Seventy‐seven patients were revisions of prior ESS. The mean number of sinuses dilated per subject was 2.7. Technical dilation success was 93.3%, 90.5%, and 93.7% for maxillary, sphenoid, and frontal sinuses, respectively. SNOT‐20 and LMK computed tomography (CT) scoring showed statistically significant improvement at 24 weeks (p < 0.0001) and clinically significant improvement in quality of life. The procedure was reported as tolerable or highly tolerable by 82.3% of patients. There were 0.15 postoperative debridements per patient and the majority returned to normal activity within 48 hours. One (0.5%) procedure‐related adverse event related to periorbital swelling was reported, which spontaneously resolved shortly after the procedure without further sequelae.ConclusionPerformance of ESS with BSD in the office under local anesthesia is feasible, well‐tolerated, safe, and effective. Twenty‐four week follow‐up demonstrates clinical and statistical improvement in patient quality of life and radiographic outcomes.
City Map of AncientEpomanduodurum(Mandeure–Mathay, Franche‐Comté, Eastern France): Contribution of Geophysical Prospecting Techniques
Authors: Bossuet G. , Thivet M. , Trillaud S. , Marmet E. , Laplaige C. , Dabas M. , Hullin G. , Favard A. , Combe L. , Barres E. , Lacaze S. , Aubry L. , Chassang M. , Mourot A. , Camerlynck C. 
Published: November 2012

ABSTRACTThis study presents the main results of the geophysical survey of ancientEpomanduodurum, at Mandeure–Mathay, Eastern France. In northeastern Gaul,Epomanduodurumis a site of a major scientific interest for the understanding of past settlements and territorial formation at the end of the Iron Age and during the Roman period. The site, including a Roman urban centre and two suburbs containing workshops, occupies more than 500 ha inside and beyond a meander of the River Doubs. From the beginning of survey in 2001, several methods (fast electrical imaging automatic resistivity profiling system, ground penetrating radar, magnetic and electromagnetic mapping) have been performed on a large scale in order to precisely identify the vast extent and structure of the Roman town. The interpretation of the geophysical data was carried out using a combination of different data sources, including ancient maps and excavations recently conducted on restricted areas. The overall organization of an artisan quarter of 8 ha was revealed along a main Roman road leading to the left bank of the River Doubs. On the other bank, a river fortification and a vast monumental religious complex were recognized in the southern part of the Roman town. The geophysical imaging shows that the sacred area includes several temples or groups of temples radiating from the theatre. Other new buried structures probably corresponding to annexes, chapels and altars were also detected within this sacred area of 10 ha bound on three sides by a wall enclosure with monumental passages. In a surveyed area of 70 ha, geophysical prospecting techniques provided a clear overall image of the Roman urbanism revealed through a rectangular road network and street system delimiting insulae of variable sizes. The detection along the River Doubs of buildings similar to storehouses attests to fluvial transport and also suggests the likely existence of a port. More generally, the combination on a large scale of geophysical prospecting, aerial photographs and excavations allowed a new plan of the urban structure of the ancient town ofEpomanduodurumto be produced for an area of over 300 ha. Copyright © 2012 John Wiley & Sons, Ltd.
From Osmium Hydrido Vinylidene to Osmacycles: The Key Role of Osmabutadiene Intermediates
Authors: Zhao Qianyi , Cao Xiao‐Yu , Wen Ting Bin , Xia Haiping 
Published: November 2012

AbstractOsmium hydrido vinylidene 1 shows diverse cyclization reactivity with activated terminal alkynes. Treatment of 1 with HCCCOR′ (R′=OEt and Me) gave osmafurans 3 a and 3 b via osmium alkenyl/vinylidenes 2 a and 2 b. In addition, 1 reacted with HCCCH(OH)CCH to yield osmabenzene 4, in which the alkynol acted as a C5 fragment to cyclize with 1. Mechanistic analysis indicates that these reactions and the previous formal [3+3] cycloadditions between 1 and HCCCH(OH)R (R=Ph, Et, and vinyl) or HCCCH(OEt)2 all go through similar osmabutadiene intermediates. Subsequently, the intermediates either took a “coordination and cyclization” process or a “carbon–carbon coupling” path to cyclization, depending on the coordination ability of substituents on the terminal alkenyl carbon atom.
The Need for Accountability to, and Support for, Children of Men on Domestic Violence Perpetrator Programmes
Authors: Alderson Susan , Westmarland Nicole , Kelly Liz 
Published: November 2012

Many domestic violence perpetrator programmes have incorporated the issue of children's safety and the harmful parenting of domestically violent fathers within their programme content. However, little attention has been paid to the services offered to, and possible outcomes for, the children of men on such programmes. This paper draws on a survey of 44 domestic violence services and 73 interviews with men who were on, or had completed, a programme, ex/partners, programme workers and programme funders/commissioners, to explore how a positive outcome for children might be conceptualised.Despite a desire to improve the situation of children, very few organisations provided a direct support service to the children of men on programmes. Work with men and support for their ex/partners operated as some form of proxy service to children. Many men had not told their children they were attending a perpetrator programme, and we suggest that more encouragement to do so would improve perpetrator accountability and respect for children. A positive outcome for children from their father's involvement on the programme is identified as having three dimensions: changes in the father that would benefit children; changes in the father‐child relationship; and changes in the child's functioning. Copyright © 2012 John Wiley & Sons, Ltd.‘Very few organisations provided a direct support service to the children of men on programmes’Key Practitioner Messages:A positive outcome for children whose father has participated on a domestic violence perpetrator programme has a range of dimensions.Men on domestic violence perpetrator programmes should be actively encouraged and supported to tell their children about their attendance.There is a need for more direct support services for the children of men on domestic violence perpetrator programmes.‘Should be actively encouraged and supported to tell their children about their attendance’
Hydrodynamic and Mass Transfer Performances of a New SiC Foam Column Tray
Authors: Zhang L. , Liu X. , Li H. , Sui H. , Li X. , Zhang J. , Yang Z. , Tian C. , Gao G. 
Published: November 2012

AbstractThe hydrodynamic performance of a novel SiC foam column tray (SFCT), made of thin slices of SiC foam material with a high specific surface area, is investigated. The performance parameters include pressure drop, entrainment, weeping, and clear liquid height. The tests are carried out with an air‐water system under atmospheric pressure. The mass transfer efficiency of the new SiC foam column tray is studied in a stainless‐steel plate column. The results provide some important parameters for the development of this innovative SiC foam tray.
Reaction Mechanism and Kinetics of 1,8‐Diazabicyclo[5.4.0]undec‐7‐ene and Carbon Dioxide in Alkanol Solutions
Authors: Öztürk M. Ç. , Ume C. S. , Alper E. 
Published: November 2012

AbstractCarbon dioxide‐binding organic liquids (CO2BOL) are a new class of solvents with advantageous properties such as high boiling points, low specific heats, high absorption capacities, and easily reversible reactions. In order to implement these solvents in processes, the reaction characteristics must be determined a priori. This work presents an analysis of the rate constants and activation energies of the reaction between carbon dioxide and 1,8‐diazabicyclo[5.4.0]undec‐7‐ene (DBU) in 1‐hexanol and 1‐propanol. The reactions were found to comply with a termolecular reaction mechanism and exhibited pseudo‐first‐order behavior in the presence of excess DBU and 1‐alkanol. It was concluded that DBU‐based CO2BOL are environmentally friendly and easy‐to‐handle solvents that may provide great flexibility and improvements over conventional carbon dioxide absorption processes.
Investigation of the Substrate Range of CYP199A4: Modification of the Partition between Hydroxylation and Desaturation Activities by Substrate and Protein Engineering
Authors: Bell Stephen G. , Zhou Ruimin , Yang Wen , Tan Adrian B. H. , Gentleman Alexander S. , Wong Luet‐Lok , Zhou Weihong 
Published: November 2012

AbstractThe cytochrome P450 enzyme CYP199A4, from Rhodopseudomonas palustris HaA2, can efficiently demethylate 4‐methoxybenzoic acid. It is also capable of oxidising a range of other related substrates. By investigating substrates with different substituents and ring systems we have been able to show that the carboxylate group and the nature of the ring system and the substituent are all important for optimal substrate binding and activity. The structures of the veratric acid, 2‐naphthoic acid and indole‐6‐carboxylic acid substrate‐bound CYP199A4 complexes reveal the substrate binding modes and the side‐chain conformational changes of the active site residues to accommodate these larger substrates. They also provide a rationale for the selectivity of product oxidation. The oxidation of alkyl substituted benzoic acids by CYP199A4 is more complex, with desaturation reactions competing with hydroxylation activity. The structure of 4‐ethylbenzoic acid‐bound CYP199A4 revealed that the substrate is held in a similar position to 4‐methoxybenzoic acid, and that the Cβ CH bonds of the ethyl group are closer to the heme iron than those of the Cα (3.5 vs. 4.8 Å). This observation, when coupled to the relative energies of the reaction intermediates, indicates that the positioning of the alkyl group relative to the heme iron may be critical in determining the amount of desaturation that is observed. By mutating a single residue in the active site of CYP199A4 (Phe185) we were able to convert the enzyme into a 4‐ethylbenzoic acid desaturase.
Design and Preparation of Platinum–Acetylide Organogelators Containing Ethynyl–Pyrene Moieties as the Main Skeleton
Authors: Xu Xing‐Dong , Zhang Jing , Yu Xudong , Chen Li‐Jun , Wang De‐Xian , Yi Tao , Li Fuyou , Yang Hai‐Bo 
Published: November 2012

AbstractA series of new platinum‐acetylide complexes containing ethynyl‐pyrene moieties as the main skeleton were synthesized and characterized. The investigation of the absorption and emission spectra of these complexes revealed that the extension of the molecular size with the introduction of different numbered platinum‐acetylide fragments can efficiently tune the absorption and emission bands from the UV to the longer wavelength region. Moreover, the gelation properties of these complexes were investigated by the “stable‐to‐inversion‐of‐a‐test‐tube” method. Most newly designed platinum‐acetylide compounds presented a stable gel‐formation property in some of the tested solvents. The morphology of the xerogels was investigated by scanning electron microscopy (SEM). Furthermore, the concentration‐ and temperature‐dependent absorption and emission properties of these complexes were investigated, which support the formation of J‐type assemblies during the aggregation process. More importantly, it was found that the complexes 4 a‐C6, 4 a, and 4 a‐C18 with four platinum‐acetylide fragments presented potential applications as luminescent organometallic gels.
Mechanical and functional properties of epothilone‐stabilized microtubules
Authors: Yu Dezhi , Pessino Veronica , Kuei Steve , Valentine Megan T. 
Published: November 2012

AbstractUsing a suite of biophysical tools, we assess the mechanical, structural, and functional properties of microtubules (MTs) stabilized by the chemotherapeutic compounds epothilone‐A, epothilone‐B, and taxol in vitro. We demonstrate that MTs stabilized by epothilone‐A or epothilone‐B are competent to bind tau proteins and support kinesin translocation. Kinesin speed is sensitive not only to the type of small molecule stabilizer used but also to the presence of the essential MT‐associated protein tau. Epothilone‐stabilized MTs are substantially less stiff than taxol‐stabilized MTs. The addition of tau proteins to MTs stabilized by either epothilone compound or taxol further reduces stiffness. Taken together, these results suggest that small molecule stabilizers do not simply stabilize a “native” MT structure, but rather they modulate the structure, function, and mechanics of the MTs they bind. This may have important consequences to the therapeutic use of these agents in cancer chemotherapies. © 2012 Wiley Periodicals, Inc
Design and implementation of a contact‐less power charger for robot applications
Authors: Cheng Shih‐Jen , Chiu Huang‐Jen , Lo Yu‐Kang , Kuo Shu‐Wei , Jen Kuo‐Kuang , Fu Kuo‐Sheng , You Gwo‐Huei , Chen Kun‐Feng , Kao Chien‐Min 
Published: November 2012

ABSTRACTIn this paper, a contact‐less power charger for robot applications is studied and developed. Contact‐less charging can be achieved by a separable transformer design. The transformer primary core is in the charger unit, and the secondary core is in the robots. The transformer air‐gap is equal to the distance between these two parts. By theoretical analysis, software simulations, and circuit implementation, the relationship among the transformer's coupling coefficient, the core geometry, and gap are formulated. In addition, a high‐efficiency circuit topology for the studied contact‐less charger is fulfilled. It is anticipated that the research results of this paper can contribute to the development of the contact‐less charging techniques for robot systems. Copyright © 2012 John Wiley & Sons, Ltd.
Endoreduplication intensity as a marker of seed developmental stage in the Fabaceae
Authors: Rewers Monika , Sliwinska Elwira 
Published: November 2012

AbstractFlow cytometry (FCM) can be used to study cell cycle activity in developing, mature and germinating seeds. It provides information about a seed's physiological state and therefore can be used by seed growers for assessing optimal harvest times and presowing treatments. Because an augmented proportion of 4C nuclei usually is indicative of high mitotic activity, the 4C/2C ratio is commonly used to follow the progress of seed development and germination. However, its usefulness for polysomatic (i.e., containing cells with different DNA content) seeds is questioned. Changes in cell cycle/endoreduplication activity in developing seeds of five members of the Fabaceae were studied to determine a more suitable marker of seed developmental stages for polysomatic species based on FCM measurements. Seeds of Phaseolus vulgaris, Medicago sativa, Pisum sativum, Vicia sativa, and Vicia faba var. minor were collected 20, 30, 40, 50, and 60 days after flowering (DAF), embryos were isolated and the proportion of nuclei with different DNA contents in the embryo axis and cotyledon was established. The ratios 4C/2C and (Σ>2C)/2C were calculated. Dried seeds were subjected to laboratory germination tests following international seed testing association (ISTA) rules. Additionally, the absolute nuclear DNA content was estimated in the leaves of the studied species. During seed development nuclei with DNA contents from 2C to 128C were detected; the endopolyploidy pattern depended on the species, seed organ and developmental stage. The cell cycle/endoreduplication parameters correlated negatively with genome size. The (Σ>2C)/2C ratio in the cotyledons reflected the seed developmental stage and corresponded with seed germinability. Therefore, this ratio is recommended as a marker in polysomatic seed research and production instead of the 4C/2C ratio, which does not consider the occurrence of endopolyploid cells. © 2012 International Society for Advancement of Cytometry
Kinetic analysis of intracellular Hoechst 33342—DNA interactions by flow cytometry: Misinterpretation of side population status?
Authors: Smith Paul J. , Wiltshire Marie , Chappell Sally C. , Cosentino Laura , Burns Philip A. , Pors Klaus , Errington Rachel J. 
Published: November 2012

AbstractWe outline a simple approach involving instrument setup and calibration for the analysis of Hoechst dye 33342‐loading in human cell lines for exploring heterogeneity in dye efflux efficiency and the status of side population (SP) A549 lung cancer cells. Dual excitation 488 nm/multiline UV (351–364 nm) flow cytometry was used to confirm ABCG2‐specific inhibition of dye efflux using Fumitremorgin C. Transporter gene expression, assayed by qRT‐PCR, confirmed higher expression of ABCG2 versus ABCB1, reiterated in a cloned subline. Coexpression of aldehyde dehydrogenase genes ranked as aldehyde dehydrogenase class 1A1 (ALDH1A1) > ALDH3A1 > ALDH3, relative expression of all genes was again reiterated in a cloned subline. Permeabilized cells were used to create red:violet (660:405 nm Em wavelengths) ratiometric references for mapping temporal changes in Hoechst 33342–DNA fluorescence in live cells. A live cell “kinetic SP gate” tracked progressive dye loading of the whole population and coapplication of the far red (>695 nm wavelength) fluorescing dye DRAQ7 enabled viable cell gating. Kinetic gating revealed a continuum for dye accumulation suggesting that SP enumeration is critically dependent upon the nonlinear relationship of the spectral shift with progressive dye–DNA binding and thus requires accurate definition. To this end, permeabilized cell reference samples permit reproducible instrument setup, guide gate boundaries for SP and compromised cells, and offer a simple means of comparing SP enumeration across laboratory sites/platforms. Our approach reports the dynamic range for the spectral shift, revealing noninformative staining conditions and explaining a source of variability for SP enumeration. We suggest that live cell kinetic sorting of all cells with the same dye:DNA load but with differences in efflux capacity can be used to explore drug resistance capability without prejudice. The SP phenotype should be regarded as a kinetic parameter and not a fixed characteristic—critical for functional assay design and the interpretation of heterogeneity.
© 2012 International Society for Advancement of Cytometry
A Fluorescent Dissymmetric Thiosemicarbazone Ligand Containing a Hydrazonequinoline Arm and Its Complexes with Cadmium and Mercury
Authors: Calatayud David G. , López‐Torres Elena , Mendiola M. Antonia 
Published: November 2012

AbstractA new dissymmetric thiosemicarbazone ligand containing a hydrazonequinoline arm, H2AMeTsQ, and its chloride salt, [H3AMeTsQ]Cl, were synthesized. Nine new complexes of cadmium and mercury with different structural characteristics were obtained under different reaction conditions as the ligand charge can be controlled by varying the amount of base. 113Cd and 199Hg NMR spectroscopy together with X‐ray diffraction indicated that the complexes form monomeric and dimeric structures and even a coordination polymer. Fluorescence spectroscopy showed that both protonated and neutral forms of the ligand were fluorescent as well as some of their cadmium and mercury derivatives. The fluorescence intensity decreased upon complexation and in some complexes a shift of the emission maximum was also observed.
Developments in the Coordination Chemistry of Phosphinines
Authors: Müller Christian , Broeckx Leen E. E. , de Krom Iris , Weemers Jarno J. M. 
Published: November 2012

AbstractThis microreview describes progress in the development and coordination chemistry of donor‐functionalized phosphinines – the phosphorus analogues of pyridines – that has been made during the last five years. The stepwise assembly of 2,4,6‐triarylphosphinines starting from functionalized benzaldehyde and acetophenone derivatives allows the incorporation of additional substituents into specific positions of the aromatic phosphorus heterocycle. This strategy can be used to synthesize chelating phosphinines, which is an essential aspect especially for the preparation of phosphinine‐based transition metal complexes containing metal centres in medium‐to‐high oxidation states. Access to such coordination compounds used to be very difficult because monodentate phosphinines cannot be used for this purpose, due to their weak σ‐donor properties but strong π‐accepting capacities. Moreover, metal complexes of less highly substituted phosphinines turned out to be extremely sensitive to nucleophilic attack, making their straightforward synthesis, characterization and application rather unattractive. The recent strategies in this area facilitate synthetic access to a completely new set of phosphinine‐metal complexes for the first time, leading to a much broader scope for potential applications. New developments in the areas of homogeneous catalysis and materials sciences can consequently be foreseen in the near future.
Synthesis and Characterization of Sterically Enlarged Hoveyda‐Type Olefin Metathesis Catalysts
Authors: Shahane Saurabh , Toupet Loïc , Fischmeister Cédric , Bruneau Christian 
Published: November 2012

AbstractA series of four ruthenium‐based olefin metathesis catalysts has been prepared. These new complexes were designed with nanofiltration in organic media in mind; steric enlargement and functionalisation by means of polar ethylene glycol chains were incorporated. New complexes based on the stable 2nd generation Hoveyda‐type architecture and featuring substitution either on the NHC backbone or on the N‐aryl substituent of the NHC have been prepared and fully characterized. The application of these complexes in a series of olefin metathesis transformations revealed that these modified catalysts retained activity on par with the parent Hoveyda catalyst thus validating the disclosed ligand design.
Intramolecular Glycosidation by Click Reaction Mediated Spacer Generation Followed by Spacer Cleavage
Authors: Kumar Amit , Geng Yiqun , Schmidt Richard R. 
Published: November 2012

Abstract2‐O‐Propargyl‐substituted glycosyl donors andO‐(2‐azidobenzyl)‐substituted acceptors having a vicinal hydroxy group readily underwent the click reaction. Intramolecular glycosidation withN‐iodosuccinimide/trifluoromethansulfonic acid as the activating system afforded β‐(1–3)‐ and α‐(1–2)‐linked disaccharides as part of 14‐membered macrocycles. Descriptors for these reactions are proposed that consider the donor and acceptor attachment sites and the stereochemistry of the functional groups. Investigation of the influence of 2‐O‐linked 1‐aryl‐1,2,3‐triazol‐4‐ylmethyl groups, as contained in the spacer, on the anomeric selectivity exhibited no anchimeric assistance. In addition, it was shown that the spacer group can be readily cleaved under Birch reduction conditions.