bluuebunny/crossref_metadata_2025_split huggingface dataset:
Laboratory validation of the automated diagnosis of intestinal parasites via fecal sample processing for the recovery of intestinal parasites through the dissolved air flotation technique
Authors: Soares Felipe Augusto , Suzuki Celso Tetsuo Nagase , Sabadini Edvaldo , Falcão Alexandre Xavier , de Oliveira Baccin Amanda , de Melo Leyva Cecília Vieira , Gomes Jancarlo Ferreira 
Published: August 2024

Abstract
Background
Techniques for diagnosing intestinal parasites need technological advancements in the preanalytical (collection/processing) and analytical (detection) stages. The dissolved air flotation (DAF) technique effectively recovers parasites from processed feces for routine diagnosis. Artificial intelligence (AI) is a practical and affordable alternative to modernize the analysis stage of microscopy images and generates high efficiency in the parasitological examination of feces.

Methods
The objective of this study was to standardize a laboratory protocol for stool processing using the DAF technique in conjunction with an automated diagnosis of intestinal parasites (DAPI) system. A total of 400 samples were obtained to perform the tests with the use of DAF to verify the recovery of the parasites as a function of the chemical reagent (polymer and surfactant), the volume of the flotation tube, and standardization of smear assembly on a microscopy slide, with automated analysis by DAPI. The DAF protocol that obtained the most satisfactory results in terms of parasite recovery (P < 0.05) and slide positivity was compared with the Three Fecal Test (TF-Test) protocol with manual (microscopists) and automated (DAPI) evaluation. We compared the sensitivity with the modified TF-Test technical protocol and the diagnostic agreement with the gold standard (Kappa) result.

Results
There was no significant difference in the parasite recovery between the 10 ml and 50 ml tubes (P > 0.05). The surfactants showed a range of parasite recoveries between 41.9% and 91.2% in the float supernatant. We obtained a maximum positivity of 73% of the assembled slides when we applied DAF processing with 7% CTAB surfactant and 57% positivity with the modified TF-Test technique. Regarding diagnostic performance, the TF-Test-modified and DAF techniques used in fecal processing for subsequent computerized analysis by AI presented sensitivities of 86% and 94%, with kappa agreements of 0.62 and 0.80 (substantial), respectively.

Conclusions
The DAF protocol defined in this study and the DAPI system are innovative processes for parasite recovery and fecal debris elimination that are favorable for effectively detecting pathogenic structures in laboratory diagnosis.

Graphical Abstract
Comprehensive multiparametric analysis of human deepfake speech recognition
Authors: Malinka Kamil , Firc Anton , Šalko Milan , Prudký Daniel , Radačovská Karolína , Hanáček Petr 
Published: August 2024

AbstractIn this paper, we undertake a novel two-pronged investigation into the human recognition of deepfake speech, addressing critical gaps in existing research. First, we pioneer an evaluation of the impact of prior information on deepfake recognition, setting our work apart by simulating real-world attack scenarios where individuals are not informed in advance of deepfake exposure. This approach simulates the unpredictability of real-world deepfake attacks, providing unprecedented insights into human vulnerability under realistic conditions. Second, we introduce a novel metric to evaluate the quality of deepfake audio. This metric facilitates a deeper exploration into how the quality of deepfake speech influences human detection accuracy. By examining both the effect of prior knowledge about deepfakes and the role of deepfake speech quality, our research reveals the importance of these factors, contributes to understanding human vulnerability to deepfakes, and suggests measures to enhance human detection skills.
Paralleled grazing and mowing differentially affected plant community diversity and productivity in a semi-arid grassland
Authors: Hassan Nazim , Wang Zhengwen 
Published: August 2024

Abstract
Background
Numerous previous studies have investigated the effects of grazing or mowing on plant community diversity and productivity in grasslands; however, few have deliberately made sound comparison between the effects of paralleled grazing and mowing in terms of biomass removal on plant community diversity and productivity in semi-arid grasslands. Using a 4-year field manipulative experiment, we investigated how moderate intensity of domestic cattle (Bos taurus) grazing and mowing can affect plant community diversity and productivity in the semi-arid grassland in northeastern China, with the attempt to find a better management practice.

Results
Our results showed that grazing significantly increased plant species richness by 9% but did not change plant biomass, whereas mowing did not alter plant species richness but significantly reduced total plant biomass and root biomass by 18% and 12%, respectively, and significantly altered plant community composition, reflected by 32% increase of grass to forb biomass ratio.

Conclusions
Cattle grazing exerted a neutral effect on plant biomass and a positive effect on plant species richness, suggesting that cattle grazing is a better management practice compared to the paralleled mowing, but longer-term experiments are needed to explore the lasting influences of grazing vs. mowing on grassland productivity, plant diversity and the sustainability.
Anisotropic structure at shallow depths across the Japan Trench
Authors: Tonegawa Takashi , Shiomi Katsuhiko , Takagi Ryota 
Published: August 2024

AbstractAnisotropic structures within the crust are frequently perceived to originate from stress-induced cracks, which have been mainly estimated on land through different wave speeds of orthogonally polarized S waves propagating in the anisotropic media. However, such estimations of crustal anisotropic structures in ocean areas, particularly for subduction zones around trenches, have not been investigated in detail due to the lack of long-term ocean bottom observations. In this study, we used ocean bottom seismometers of a permanent network deployed across the Japan Trench and the southern part of the Kuril Trench and applied the shear-wave splitting analysis to P-to-s converted waves extracted by receiver function analyses using teleseismic events. We estimated the anisotropic structures in marine sediments and oceanic crust for the incoming Pacific Plate and marine sediments for the overriding North American Plate. The obtained fast polarization directions for the incoming plate are mainly oriented to be parallel to the trench axis for the marine sediment and oceanic crust, which are formed by normal faults and cracks due to the upward plate bending in the outer-rise region, whereas results for marine sediments at the northern part of the Japan Trench are obliquely aligned to the trench axis. The oblique direction is consistent with the magnetic lineations of the incoming plate, indicating that ancient faults within the plate, which were formed in the shallow part of the crust during the creation of the oceanic plate at the ridge, are reactivated by the plate flexure. For the overriding plate, the fast polarization directions in the northern and southern parts of the study area are nearly normal to the trench axis. The central part shows two distinct features: the fast polarization directions parallel to the trench axis and small degrees of anisotropy. These patterns may reflect crack alignments associated with the lateral variation in postseismic crustal deformation after the 2011 Tohoku-Oki earthquake. Our results suggest substantial lateral variations in the stress field at the tip of the overriding plate along the strike direction.
Graphical Abstract
The MAP4 kinase NbM4K3 regulates immune responses in Nicotiana benthamiana
Authors: Zhang Shuangxi , Li Haijuan , Zhang Meixiang , An Yuyan 
Published: August 2024

AbstractThe mitogen-activated protein kinase kinase kinase kinase (M4K) family is evolutionarily conserved across plants and animals. In Arabidopsis, the protein kinase SIK1, an M4K member, is known to positively modulate reactive oxygen species (ROS) production during pattern-triggered immunity (PTI) by stabilizing BIK1, a key receptor-like cytoplasmic kinase (RLCK). While homologs of SIK1 exhibit conserved protein domain architectures across a range of land plants, their functional conservation remains incompletely understood. This study investigates the functional conservation and divergence of SIK1 homologs, focusing particularly on NbM4K3 in Nicotiana benthamiana. Silencing NbM4K3 resulted in an impairment of the flg22-induced ROS burst and expression of PTI marker genes. Additionally, silencing NbM4K3 led to diminished protein accumulation of RLCKs, while overexpression of the RLCKs prominently enhanced the flg22-induced ROS burst in NbM4K3-silenced plants. Furthermore, NbM4K3-silenced plants exhibited a compromised hypersensitive response (HR), reduced ROS accumulation, and diminished expression of effector-triggered immunity (ETI) marker genes when challenged with the avirulent strains Ralstonia solanacearum GMI1000 and Pseudomonas syringae DC3000, suggesting that NbM4K3 is a positive regulator of ETI. The attenuated HR phenotype observed in NbM4K3-silenced plants upon expression of RipP1 or RipE1, two avirulent type III effectors of GMI1000, further supports the affirmative role of NbM4K3 in ETI. In summary, our data indicate that the M4K NbM4K3 positively regulates both PTI and ETI in N. benthamiana, potentially by stabilizing RLCKs. These findings not only strengthen the role of M4K family in plant immunity but also suggest its potential in improving disease resistance in plants.
Low C-Reactive Protein Alleles in Hepatocyte Nuclear Factor 1A Are Associated With an Increased Risk of Cardiovascular Disease
Authors: Yang Chaochao , Ji Linong , Han Xueyao 
Published: August 2024

Abstract

Context
Rare variants in HNF1A cause both maturity onset diabetes of the young 3 (HNF1A-MODY) and reduced serum C-reactive protein (CRP) levels. Common variants of HNF1A are associated with serum CRP and type 2 diabetes mellitus (T2DM), but inconsistently with cardiovascular disease (CVD).

Objective
Our study aimed to investigate the association of low CRP alleles in HNF1A with CVD and indirectly evaluate the CVD risk of HNF1A-MODY patients because of unavailability of enough cases to study their clinical outcomes.

Methods
A literature search was performed using PubMed, Embase, and Cochrane Library databases from inception to December 2023. All relevant studies concerning the association of HNF1A with CRP, CVD, lipids, and T2DM were included. Odds ratios (ORs), 95% CIs, and study characteristics were extracted.

Results
Three common coding variants of HNF1A (rs1169288, rs2464196, and rs1169289) were examined. The minor alleles of these variants correlated with low CRP levels (OR 0.89; 95% CI, 0.86-0.91; OR 0.89; 95% CI, 0.88-0.91; OR 0.89; 95% CI, 0.88-0.91, respectively). Their low CRP alleles were associated with increased risk of CVD (OR 1.03; 95% CI, 1.03-1.04), higher low-density lipoprotein cholesterol levels (OR 1.07; 95% CI, 1.04-1.10), and elevated risk of T2DM (OR 1.04; 95%, CI 1.01-1.08).

Conclusion
Our study revealed an association between low CRP alleles in HNF1A and a high CVD risk, which indicated that antidiabetic drugs with CV benefits such as glucagon-like peptide-1 receptor agonists should be recommended as a first-line choice for HNF1A-MODY.
Communication barriers in caring for culturally diverse patients and the cultural competence of nursing staff
Authors: Charzyńska-Gula Marianna , Zych-Kwaśnik Katarzyna , Rożek Grażyna , Szulich Piotr , Sojda Agnieszka , Tomczyk Bogdan , Zielińska Marta 
Published: August 2024

Introduction:
The culturally different patient is still a new task for nursing staff. In order to realise competent and effective care for a patient from a different culture, the nurse must be able to recognise her own cultural filters, communication barriers, cultural differences, the sometimes different value system and its relevance to the patient's quality of life
Objective of the study: The aim of this study is to illustrate the communication barriers in caring for a culturally different patient in a selected group of nursing staff and to contrast them with the cultural competences possessed by this group.
Material and method: 127 nursing staff of one of the Podkarpackie hospitals were studied. A diagnostic survey with an author's survey questionnaire and the Polish version of the Cultural Competence Scale of Medical Personnel were used.
Results: Individuals with higher cultural competence in behaviour were better able to cope with communication barriers in terms of language differences and non-verbal communication. Higher cultural competence in patient-centred communication was associated with better coping with barriers in terms of cultural differences and non-verbal communication. The higher the cultural competence in practice orientation, the lower the coping with excess emotions or information and the worse the coping with the patient's culture and values conflicting with the patient's own culture and value system. Those who scored higher on the self-assessment of cultural competence simultaneously coped better with barriers concerning language differences. Those who showed higher awareness and sensitivity with regard to cultural competence coped less well with emotional excess.
Conclusions: Relatively good levels of problem solving related to communication barriers are not always accompanied by full cultural competence.
Faktor – Faktor Yang Berhubungan Dengan Kepuasan Kerja Perawat Di Instalasi Rawat Inap Rumah Sakit PKU Muhammadiyah Yogyakarta
Authors: Lisdiyanti Teti , Syam Nur Syarianingsih 
Published: August 2024

Background: As many as 40% of healthcare workers in Indonesia are nurses. Nursing services are a determining factor in the success of health services that impact the quality of hospital services. Therefore, improving service quality is related to the level of nurse job satisfaction. Objective: The purpose of this study is to identify factors related to nurse job satisfaction in the Inpatient Installation of PKU Muhammadiyah Yogyakarta Hospital.
Methods: This study applied a Cross-Sectional research design, conducted in July 2023 at PKU Muhammadiyah Yogyakarta Hospital. The research sample was inpatient nurses with a total sampling technique of 175 nurses. Researchers collected data by distributing questionnaires. In analyzing the data, univariate analysis with frequency distribution and bivariate analysis with Chi-Square test were used.
Results: The job itself is related to inpatient nurse job satisfaction, indicated by a p-value of 0.000 (p <0.05) Salary and inpatient nurse job satisfaction are related, indicated by a p-value of 0.000 (p <0.05). Job promotion is related to inpatient nurse job satisfaction, indicated by a pvalue of 0.000 (p <0.05).
Conclusion: This study concluded that nurses' job satisfaction needs to be maintained and improved through nurse career development programs and appropriate work benefits.
Efficacy and safety of autologous whole blood clot in diabetic foot ulcers: a randomised controlled trial
Authors: Snyder Robert , Nouvong Aksone , Ulloa Jesus , Wahab Naz , Treadwell Terry , Bruwer Febe , Naude Liezl , McGuire James , Reyzelman Alexander M , Graham Timothy ,   AWBC Research Team:, Lessing Rene , Lullove Eric , Ozker Emre , Pham Hau T , Pasternac Michael , Cohen Shira 
Published: August 2024

Objective: Diabetic foot ulcers (DFUs) present a significant global health challenge, resulting in high morbidity and economic costs. Current available treatments often fail to achieve satisfactory healing rates, highlighting the need for novel therapies. This study evaluated the safety and efficacy of a novel autologous whole blood clot (AWBC)—a blood-based, biodegradable provisional matrix—in conjunction with standard of care (SoC) when compared to SoC alone in the treatment of hard-to-heal DFUs. Method: A multicentre, prospective, blinded assessor, randomised controlled trial was conducted at 16 sites across the US, South Africa and Turkey. A cohort of patients with hard-to-heal DFUs was enrolled and randomised to either the AWBC group or the control group. The primary endpoint was complete wound closure at 12 weeks, while secondary endpoints included time to heal and percentage area reduction (PAR) at four and eight weeks. Data were analysed using both intention-to-treat (ITT) and per-protocol (PP) populations. Results: The cohort included 119 patients. AWBC treatment resulted in a significantly higher healing rate compared to the control in both ITT (41% versus 15%, respectively; p=0.002) and PP populations (51% versus 18%, respectively; p=0.0075). AWBC treatment also resulted in a shorter mean time to heal and higher durability of wound closure. Safety analysis showed a similar incidence of adverse events (AEs) between groups, with no device-related AEs. Conclusion: The AWBC system, by modulating the wound microenvironment and providing a functional extracellular matrix, offered a promising new approach to treating hard-to-heal DFUs, demonstrating superior healing outcomes compared to SoC alone in this study.
Synergistic effects of transcutaneous spinal stimulation and neuromuscular electrical stimulation on lower limb force production: Time to deliver
Authors: Steele Alexander G. , Vette Albert H. , Martin Catherine , Masani Kei , Sayenko Dimitry G. 
Published: August 2024

Background
Transcutaneous spinal stimulation (TSS) and neuromuscular electrical stimulation (NMES) can facilitate self-assisted standing in individuals with paralysis. However, individual variability in responses to each modality may limit their effectiveness in generating the necessary leg extension force for full body weight standing. To address this challenge, we proposed combining TSS and NMES to enhance leg extensor muscle activation, with optimizing timing adjustment to maximize the interaction between the two modalities.

Methods
To assess the effects of TSS and NMES on knee extension and plantarflexion force, ten neurologically intact participants underwent three conditions: (1) TSS control, (2) NMES control, and (3) TSS + NMES. TSS was delivered between the T10 and L2 vertebrae, while NMES was delivered to the skin over the right knee extensors and plantarflexors. TSS and NMES were administered using a 15 Hz train of three 0.5 ms biphasic pulses. During the TSS + NMES condition, the timing between modalities was adjusted in increments of ¼ the interval within a 15 Hz frequency, i.e., 66, 49.5, 33, 16.5, and 1 ms.

Results
NMES combined with TSS, produced synergistic effects even on non-targeted muscle groups, thereby promoting leg extension across multiple joints in the kinematic chain. The sequence of NMES or TSS trains relative to each other did not significantly impact motor output. Notably, a delay of 16.5 to 49.5 ms between interleaved TSS and NMES pulses, each delivered at 15 Hz, results in more robust and synergistic responses in knee extensors and plantarflexors.

Conclusions
By adjusting the timing between TSS and NMES, we can optimize the combined use of these modalities for functional restoration. Our findings highlight the potential of integrated TSS and NMES protocols to enhance motor function, suggesting promising avenues for therapeutic applications, particularly in the rehabilitation of individuals with SCI.
FENOMENA BODYSHAMING PADA MAHASISWI BKI IAIN SURAKARTA
Authors: Putri Yoan Rachmawati , Lessy Zulkipli 
Published: August 2024

The behavior known as "body shaming" is often taken for granted. Many people believe that it is acceptable to comment about other people's bodies and physical appearance; however, they do not understand that their actions can harm another person or contribute to the development of psychological disorders in that person. Each person is unique, and one of the distinguishing characteristics of him/her is his/her freedom to choose his/her own body, including the right to be fat, thin, or any other body type. The purpose of this study was to determine the phenomenon of body shaming among Bimbingan Konseling Islam (BKI) female students of class 2017 at IAIN Surakarta (now UIN Raden Mas Said). This study used a qualitative descriptive technique. The four women who volunteered to participate in the study were all familiar with the idea of shame. Data collection methods used in this study were interviews, observations, and questionnaires. The findings reveal that (1) bullying is similar to body shaming, (2) body shaming can prohibit people from participating in social activities because it makes them feel insecure, and (3) negative responses to body shaming can cause unpleasant sensations. Keywords: Body Shaming, Students of IAIN Surakarta, Phenomenon.
PENGARUH BIMBINGAN PARENTING TERHADAP PENINGKATAN KEMAMPUAN ORANG TUA DALAM MEMBIMBING TUGAS PERKEMBANGAN MORAL DAN NILAI KEAGAMAAN: STUDI TIGA PERSPEKTIF KASUS DI BANDUNG, INDONESIA
Authors: Indriyanti  , Sugandi Miharja  , Bahrudin  
Published: August 2024

Parenting guidance is a crucial concern for parents as they play a significant role in fostering their children’s moral and religious development, which in turn contributes to their academic success. However, numerous cases indicate a lack of parental involvement in cultivating their children's upbringing, leading to deficiencies in parenting skills. This study aims to enhance the understanding of parenting skills in guiding children towards the development of moral and religious values. Employing a multiple-case study approach, data from TK IT Little Moslem, TK Labschool UPI, and RA Syifaaush Shuduur in Bandung, West Java, Indonesia, were analyzed. Through a quantitative method involving 101 respondents, the study reveals that parenting guidance significantly influences the development of moral and religious values by approximately 61%, while other factors contribute to around 39% of the impact. The findings underscore the pivotal role of parental guidance in enhancing children's learning abilities and fostering an early understanding of morality and religious values. As a result, this research contributes to the existing literature by exploring the nexus between ethics and religions, particularly in the context of parental and teacher guidance in nurturing children during their formative years.

Keywords: Parenting Guidance, Guiding Ability and Moral Religious Values.
DynaHB: A Communication-Avoiding Asynchronous Distributed Framework with Hybrid Batches for Dynamic GNN Training
Authors: Song Zhen , Gu Yu , Sun Qing , Li Tianyi , Zhang Yanfeng , Li Yushuai , Jensen Christian S. , Yu Ge 
Published: August 2024

Dynamic Graph Neural Networks (DGNNs) have demonstrated exceptional performance at dynamic-graph analysis tasks. However, the costs exceed those incurred by other learning tasks, to the point where deployment on large-scale dynamic graphs is infeasible. Existing distributed frameworks that facilitate DGNN training are in their early stages and experience challenges such as communication bottlenecks, imbalanced workloads, and GPU memory overflow.
We introduce DynaHB, a distributed framework for DGNN training using so-called Hybrid Batches. DynaHB reduces communication by means of vertex caching, and it ensures even data and workload distribution by means of load-aware vertex partitioning. DyanHB also features a novel hybrid-batch training mode that combines vertex-batch and snapshot-batch techniques, thereby reducing training time and GPU memory usage. Next, to further enhance the hybrid batch based approach, DynaHB integrates a reinforcement learning-based batch adjuster and a pipelined batch generator with a batch reservoir to reduce the cost of generating hybrid batches. Extensive experiments show that DynaHB is capable of up to a 93× and an average of 8.06× speedups over the state-of-the-art training framework.