bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporal transcriptome and metabolome study revealed molecular mechanisms underlying rose responses to red spider mite infestation and predatory mite antagonism
Authors: Cai Yanfei , Shi Ziming , Zhao Peifei , Yang Yingjie , Cui Yinshan , Tian Min , Wang Jihua 
Published: August 2024

IntroductionRed spider mite (Tetranychus urticae) infestation (SMI) is a detrimental factor for roses grown indoors. Although predatory mite (Neoseiulus californicus) antagonism (PMA) is often utilized to alleviate SMI damage, little is known about the defensive response of greenhouse-grown roses to SMI and the molecular mechanism by which PMA protects roses.MethodsTo determine the transcriptome and metabolome responses of roses to SMI and PMA, the leaves of a rose cultivar (“Fairy Zixia/Nightingale”) were infested with T. urticae, followed by the introduction of predator mite. Leaf samples were collected at various time points and subjected to transcriptome and metabolome analyses.ResultsWe found that 24 h of SMI exerted the most changes in the expression of defense-related genes and metabolites in rose leaves. KEGG pathway analysis of differentially expressed genes (DEGs) and metabolites revealed that rose responses to SMI and PMA were primarily enriched in pathways such as sesquiterpenoid and triterpenoid biosynthesis, benzoxazinoid biosynthesis, stilbenoid, diarylheptanoid and gingerol biosynthesis, phytosterol biosynthesis, MAPK signaling pathway, phenylpropanoid biosynthesis, and other pathways associated with resistance to biotic stress. Rose reacted to SMI and PMA by increasing the expression of structural genes and metabolite levels in phytosterol biosynthesis, mevalonate (MVA) pathway, benzoxazinoid biosynthesis, and stilbenoid biosynthesis. In addition, PMA caused a progressive recover from SMI, allowing rose to revert to its normal growth state. PMA restored the expression of 190 essential genes damaged by SMI in rose leaves, including transcription factors DRE1C, BH035, MYB14, EF110, WRKY24, NAC71, and MY108. However, after 144 h of PMA treatment, rose responsiveness to stimulation was diminished, and after 192 h, the metabolic levels of organic acids and lipids were recovered in large measure.ConclusionIn conclusion, our results offered insights on how roses coordinate their transcriptome and metabolome to react to SMI and PMA, therefore shedding light on how roses, T. urticae, and N. californicus interact.
Impact of fertilization depth on sunflower yield and nitrogen utilization: a perspective on soil nutrient and root system compatibility
Authors: Ren Wenhao , Li Xianyue , Liu Tingxi , Chen Ning , Xin Maoxin , Liu Bin , Qi Qian , Li Gendong 
Published: August 2024

IntroductionThe depth of fertilizer application significantly influences soil nitrate concentration (SNC), sunflower root length density (RLD), sunflower nitrogen uptake (SNU), and yield. However, current studies cannot precisely capture subtle nutrient variations between soil layers and their complex relationships with root growth. They also struggle to assess the impact of different fertilizer application depths on sunflower root development and distribution as well as their response to the spatial and temporal distribution of nutrients.MethodsThe Agricultural Production Systems sIMulator (APSIM) model was employed to explore the spatial and temporal patterns of nitrogen distribution in the soil at three controlled-release fertilizer (CRF) placement depths: 5, 15, and 25 cm. This study investigated the characteristics of the root system regarding nitrogen absorption and utilization and analyzed their correlation with sunflower yield formation. Furthermore, this study introduced the modified Jaccard index (considering the compatibility between soil nitrate and root length density) to analyze soil-root interactions, providing a deeper insight into how changes in CRF placement depth affect crop growth and nitrogen uptake efficiency.ResultsThe results indicated that a fertilization depth of 15 cm improved the modified Jaccard index by 6.60% and 7.34% compared to 5 cm and 25 cm depths, respectively, maximizing sunflower yield (an increase of 9.44%) and nitrogen absorption rate (an increase of 5.40%). This depth promoted a greater Root Length Density (RLD), with an increases of 11.95% and 16.42% compared those at 5 cm and 25 cm, respectively, enhancing deeper root growth and improving nitrogen uptake. In contrast, shallow fertilization led to higher nitrate concentrations in the topsoil, whereas deeper fertilization increased the nitrate concentrations in the deeper soil layers. DiscussionThese results provide valuable insights for precision agriculture and sustainable soil management, highlighting the importance of optimizing root nitrogen absorption through tailored fertilization strategies to enhance crop production efficiency and minimize environmental impact.
“I feel like a fish out of water”: interpreting the occupational stress and well-being experiences of professional classical musicians
Authors: Willis Simone , Mellick Mikel , Neil Rich , Wasley David 
Published: August 2024

IntroductionProfessional classical musicians operate within a highly demanding environment, which includes organizational, social, and emotional demands. When not effectively coped with, these demands may cause stress and negatively impact well-being. This qualitative study explored the perceived stress and well-being experiences of professional classical musicians through a transactional theory of stress. The study employed a double hermeneutic interpretation of the lived experiences of the perceived demands faced, stress appraisals made, resources used, and the influence on well-being.MethodsSix professional classical musicians were purposefully selected for participation. Semi-structured interviews were conducted and participants reflected on two events: one they perceived as a positive experience and one that was negative. Transcripts were analyzed using Interpretative Phenomenological Analysis and Group Experiential Themes emerged.ResultsThree Group Experiential Themes were identified: (a) Performance Demands; (b) Organizational Demands; and, (c) Relationship Demands. Participants predominantly appraised demands as a threat. A small number of demands were appraised as a challenge or benefit, and the fewest demands were appraised as causing harm or loss. Participants’ appraisals were informed by underlying properties of stress appraisal such as self and other comparison, and preparation. Participants often relied on personal resources as opposed to available workplace resources. They perceived well-being to relate to stress appraisals with participants experiencing acute and long-term outcomes.DiscussionThis study offers insight into the lived experience of the occupational stress process within professional classical musicians. The findings demonstrate that organizational interventions targeted at continuing professional development and social support are appropriate to help musicians cope more effectively with demands.
Bulimia nervosa and treatment-related disparities: a review
Authors: Wilson Kim , Kagabo Robert 
Published: August 2024

IntroductionBulimia nervosa (BN) is a type of eating disorder disease usually manifesting between adolescence and early adulthood with 12 as median age of onset. BN is characterized by individuals’ episodes of excessive eating of food followed by engaging in unusual compensatory behaviors to control weight gain in BN. Approximately 94% of those with BN never seek or delay treatment. While there are available treatments, some populations do not have access. Left untreated, BN can become severe and lead to other serious comorbidities. This study is a review of randomized controlled trials to explore available treatments and related treatment disparities. The objective of this review was to identify differences among treatment modalities of BN and aide in the further treatment and research of bulimia nervosa.MethodsThis study followed narrative overview guidelines to review BN treatment studies published between 2010 and 2021. The authors used PubMed and PsychInfo databases to search for articles meeting the inclusion criteria. Search terms included phrases such as, BN treatment, BN and clinical trials, and BN and randomized clinical trials.ResultsMost of the reviewed studies had their sample sizes between 80 and 100% female with age range between 18 and 60 years old. Sample sizes were mostly between 80 and 100% white. Treatment practices included both pharmacological and psychosocial interventions, such as cognitive behavioral therapy (CBT) and limited motivational interviewing (MI). Most studies were in outpatient settings.ConclusionReviewed research shows that certain populations face disparities in BN treatment. Generally, individuals older than 60, males and racial minorities are excluded from research. Researchers and practitioners need to include these vulnerable groups to improve BN treatment-related disparities.
Perceived benefits and limitations of a psychoeducation program for patients with fibromyalgia: an interpretative phenomenological analysis
Authors: Di-Bonaventura Silvia , Ferrer-Peña Raúl , Pardo-Montero Joaquín , Férnandez-Carnero Josué , La Touche Roy 
Published: August 2024

ObjectiveTo analyze the perceived benefits and limitations of a pain psychoeducation program as a non-pharmacological treatment for patients with fibromyalgia.MethodsAn interpretative phenomenological analysis was applied to analyze the subjective experiences of 11 patients with fibromyalgia who participated in a pain psychoeducation program. This program includes educational sessions that address pain understanding, coping strategies, and relaxation techniques. Semi-structured interviews were conducted, transcribed, and analyzed using ATLAS.ti software.ResultsPatients reported significant improvements in cognitive-functional capacity and socio-emotional ability, including better disease understanding and management, emotional stability, and interpersonal relationships. Despite these benefits, they identified limitations in program individualization and insufficient coverage of certain topics, such as sexual health and legal aspects of disability. Enhanced self-management skills were evident, with observed shifts in disease perception and coping strategies.ConclusionThe psychoeducation program was viewed positively, influencing functional, cognitive, and emotional enhancements. Nonetheless, the need for increased program personalization and expanded socio-economic support was noted. Future research should focus on the long-term impacts of psychoeducation and the feasibility of tailored interventions.
Development of a TaqMan-based multiplex real-time PCR for simultaneous detection of porcine epidemic diarrhea virus, Brachyspira hyodysenteriae, and Lawsonia intracellularis
Authors: Ren Jing , Li Fujun , Yu Xue , Li Yang , Li Meng , Sha Yujie , Li Xiaowen 
Published: August 2024

IntroductionPEDV, Brachyspira hyodysenteriae, and Lawsonia intracellularis, are highly contagious diarrheal pathogens that have caused significant harm to the global swine industry. Co-infections with multiple pathogens are common, making it challenging to identify the actual causative agents depending only on clinical information. It is crucial to develop a reliable method to simultaneously detect and differentiate these pathogens.MethodsBased on the conserved regions of the M gene of PEDV, NADH oxidase gene of B. hyodysenteriae, and the 16S rDNA gene of L. intracellularis, specific probes and primers for the multiplex real-time PCR assay were designed. The concentrations of primers and probes were optimized using a matrix method.ResultsThe approach demonstrated high specificity and no cross-reactivity with major pathogens related to diarrheal diseases. It showed high sensitivity with a detection limit of 10 copies/μL for B. hyodysenteriae and L. intracellularis, and 100 copies/μL for PEDV, respectively. It also demonstrated high reproducibility and stability with low coefficients of variation. Results from the multiplex real-time PCR method were in complete agreement with the commercial singleplex real-time PCR kit for detecting PEDV, B. hyodysenteriae and L. intracellularis. Clinical data revealed single infection rates of 31.46% for PEDV, 58.43% for B. hyodysenteriae, and 98.6% for L. intracellularis. The co-infection rates were 16.85% for PEDV + B. hyodysenteriae, 31.46% for PEDV + L. intracellularis, 57.86% for B. hyodysenteriae + L. intracellularis, and 16.85% for PEDV + B. hyodysenteriae + L. intracellularis, respectively.DiscussionThe new multiplex real-time PCR method can simultaneously differentiate PEDV, B. hyodysenteriae and L. intracellularis, making it a valuable diagnostic tool for preventing and controlling infectious diseases, as well as aiding in epidemiological investigations.
Clinical Value of Peripheral Blood Gene Expression Profile and Donor-Derived Cellfree DNA for Identifying Persistent Rejection
Authors: Heilman Raymond L. , Fleming James N. , Park Sook H. , Rebello Christabel , Kleiboeker Steve , Holman John , Friedewald John J. 
Published: August 2024

Key Points

Peripheral blood biomarkers may have value in monitoring kidney transplant recipients after treatment of acute rejection.The donor-derived cellfree DNA may be more sensitive to identifying antibody-mediated rejection and gene expression profile may be more sensitive to identifying acute cellular rejection.

Background
Persistent rejection is an increasingly recognized barrier to long-term kidney allograft survival. A noninvasive method to help identify patients with persistent rejection in need of biopsy would be valuable.

Methods
This was a post hoc analysis of a multicenter observational study. Patients who had a biopsy-proven acute rejection, had another biopsy within 9 months (270 days), and had a biopsy-paired biomarker sample were included.

Results
A total of 64 index rejections in 58 patients with repeat biopsies were identified with a median time to repeat biopsy of 100 days. Persistent rejection was present in 61%; 69% of follow-up biopsies were performed in clinically stable patients. Peripheral blood gene expression profile (GEP) demonstrated a sensitivity of 59%, specificity of 76%, positive predictive value (PPV) of 79%, and negative predictive value of 54%. Donor-derived cellfree DNA (dd-cfDNA) demonstrated a sensitivity of 62%, specificity of 86%, PPV of 88%, and negative predictive value of 56%. For repeat biopsies within 90 days of rejection in clinically stable patients (63% of repeat biopsies), both GEP and dd-cfDNA had specificities and PPVs of 100%. GEP was more likely to be positive in T-cell–mediated rejection while dd-cfDNA was more likely to be positive in antibody-mediated rejection.

Conclusions
Both GEP and dd-cfDNA may have utility in identifying clinically stable patients with persistent rejection in need of biopsy; however, they identify different types of rejections.

Clinical Trial registration number:

NCT01531257.
HUBUNGAN PENGETAHUAN IBU TENTANG MANFAAT BERMAIN DENGAN PERKEMBANGAN ANAK PRASEKOLAH DI RA MAMBAUL ULUM GEDUNG KARYA JITU LAMPUNG
Authors: Sekar Ningrum Allania Hanung Putri , Aji Pamungkas Atik Mahmudah , Paramita Neni 
Published: August 2024

Pada tahun 2019 sekitar 23,1%. anak balita usia dini di Indonesia menderita penyimpangan perkembangan seperti penyimpangan dalam motorik kasar, motorik halus, serta penyimpangan mental emosional. Dari hasil studi pendahuluan, didapatkan ternyata ibu tidak memahami manfaat bermain. Tujuan penelitian untuk diketahui hubungan pengetahuan ibu tentang manfaat bermain dengan perkembangan anak usia prasekolah di RA Mambaul Ulum Gedung Karya Jitu 2023. Jenis penelitian ini adalah kuantitatif, dengan rancangan penelitian analitif korelatif. Populasi dalam penelitian ini 45 orang dengan teknik sampling purposive sampling, dan didapatkan saple 36 orang. Analisa data bivariat menggunakan uji chi square. Berdasarkan hasil penelitian ibu yang memiliki pengetahuan baik sebanyak 19 responden (52.8%). Status perkembangan anak terbanyak adalah status perkembangan meragukan sebanyak 19 orang anak (52.8%). Hasil uji statistik menggunakan chi square didapat nilai p-value 0.018, yang artinya terdapat hubungan pengetahuan ibu tentang manfaat bermain dengan perkembangan anak usia prasekolah di RA Mambaul Ulum Gedung Karya Jitu 2023. Saran kepada orangtua diharapkan dapat mengajak anaknya untuk bermain sehingga didapatkan perkembangan anak yang sesuai dengan usianya, dengan cara memberikan stimulus yang baik.
Kata Kunci : Manfaat bermain, pengetahuan, perkembangan anak
PARITAS DAN USIA IBU DENGAN ANEMIA KEHAMILAN DI PUSKESMAS NELAYAN KABUPATEN GRESIK
Authors: Arifah Dwi Fitriyatul , Mudlikah Siti 
Published: August 2024

Latarbelakang: Kekurangan asupan nutrisi dan gizi tidak seimbang penyebab terjadinya anemia defesiensi zat besi yang paling banyak diderita ibu hamil, balita dan remaja putri. Tujuan penelitian untuk mengetahui hubungan paritas dan usia ibu dengan anemia kehamilan. Metode penelitian observasional analitik, pendekatan cross sectinal. Populasi 110 ibu hamil trimester pertama. Teknik sampling purposive. Jumlah sampel 40 ibu hamil dengan rumus solving. Pengumpulan data usia dan paritas diperoleh dari buku register dan data kadar hemoglobin dari pengukuran kadar Hb ibu hamil menggunakan alat Easy-Touch Hb. Analisis uji statistic uji Rank Spearman. Hasil: ibu hamil paritas multipara 17 orang (42,5%), berusia 20-35 tahun 32 orang (80,0%), Ibu hamil tidak anemia jumlah 22 orang (55,%), anemia ringan 8 orang (20%), anemia sedang10 (25%), Analisis statistic diperoleh tidak ada hubungan paritas dengan anemia kehamilan (? 0,199) dan tidak ada hubungan usia ibu dengan anemia kehamilan di Puskesmas Nelayan Kabupaten Gresik (? 0,852). Kesimpulan : tidak ada hubungan paritas dan usia ibu dengan anemia kehamilan. Factor lain penyebab anemia meliputi asupan nutrisi, paritas, penyakit dan lainnya, maka diharapkan penanganan anemia kehamilan berdasarkan penyebabnya.
Kata Kunci : Usia, Paritas, Anemia, Kehamilan
ANALISIS FAKTOR - FAKTOR YANG BERHUBUNGAN DENGAN STATUS GIZI BALITA
Authors: Wijayanti Titik , Setiyaningsih Atik , Rismawati Rismawati 
Published: August 2024

Gizi merupakan bagian hal terpenting dalam tumbuh kembang dikarenakan erat dengan kesehatan dan kecerdasan. Masalah gizi di Indonesia terbanyak gizi kurang. dengan peningkatkan kasus pada tahun 2022 dari 17,0 % menjadi 17,1%. Di Boyolali tahun 2022 masih terdapat 4.137 balita (7,06%) mengalami stunting. Masalah kurang gizi dipengaruhi oleh penyebab langsung dan tidak langsung. Penelitian ini bertujuan untuk mengetahui faktor - faktor yang berhubungan dengan status gizi balita meliputi faktor riwayat penyakit, pola makan dan tingkat ekonomi. Penelitian yang digunakan korelasional dengan pendekatan cross sectional. Populasi seluruh ibu dan balita usia 1 - 3 tahun di Posyandu Anggrek pada bulan Mei 2023, tehnik pengambilan sampel accidental sampling. Analisis bivariatnya uji Chi Square. Respoden yang tidak memiliki riwayat penyakit status gizinya baik sebanyak 25 (64,6%), hasil uji chi square p value 0,037 < 0,05. Responden dengan pola makan baik status gizinya baik sejumlah 27 (72,9%), hasil uji chi square p value 0,007 < 0,05. Responden dengan ekonomi > UMR memiliki status gizinya baik sebanyak 17 (46%), hasil uji chi square p value 0,040 < 0,05. Ada hubungan riwayat penyakit, pola makan dan tingkat ekonomi dengan status status gizi balita.
Kata Kunci : Riwayat Penyakit, Pola Makan, Tingkat Ekonomi, Status Gizi Balita
EFEKTIFITAS TEHNIK GYM BALL TERHADAP PENURUNAN NYERI PUNGGUNG PADA IBU HAMIL TRIMESTER III
Authors: Ni’amah Siti , Sulistiyaningsih Sri Hadi 
Published: August 2024

Nyeri punggung saat hamil biasanya terjadi karena adanya peregangan tulang-tulang, terutama didaerah pinggang yang sesuai dengan bertambahnya usia kehamilan. Nyeri punggung sering terjadi karena titik dan keseimbangan ibu berubah dari kondisi semula (Andarmoyo dan Suharti, 2013). Apabila nyeri punggung ini tidak ditangani dengan baik dapat menyebabkan kualitas hidup ibu hamil menjadi buruk. Masalah ini akan berlanjut dalam bentuk cidera kambuhan atau muncul terus menerus dalam kondisi lebih buruk sesuai dengan perjalanan usia kehamilannya (Kantonis, 2017).
Gym Ball adalah terapi fisik atau latihan sederhana menggunakan bola, dimana latihan tersebut diterapkan untuk ibu hamil, ibu melahirkan dan ibu pasca melahirkan. Manfaat dari pemakaian gym ball ini adalah meningkatkan aliran darah ke rahim, plasenta dan bayi, meredakan tekanan dan dapat memberikan rasa nyaman untuk lutut dan pergelangan kaki, membantu mengurangi nyeri punggung pada ibu hamil dan mempermudah ibu hamil untuk bergerak. (Kurniawati, 2017)
Penelitian ini bertujuan untuk mengetahui pengaruh tehnik gym ball terhadap nyeri punggung pada ibu hamil Trimester III. Adapun jenis penelitian yang digunakan adalah penelitian Pre-eksperimental design dengan rancangan one group pretest posttest design. Populasi dan sampel dalam penelitian sebanyak 15 ibu hamil TM III dengan usia kehamilan 32 minggu yang diambil dengan menggunakan teknik Total sampling.
Hasil penelitian menunjukkan rata-rata frekuensi nyeri punggung sebelum intervensi adalah 1,00 dengan standar deviasi 0,791 dan rata-rata frekuensi nyeri punggung sesudah intervensi adalah 0,29 dengan standar deviasi 0,470. Hasil korelasi antara dua variabel menghasilkan angka 0,673 dengan nilai p value=0,003. Hal ini menunjukkan bahwa adanya hubungan yang kuat dengan tingkat signifikan p value < 0,05. Hasil penelitian menunjukkan bahwa korelasi penurunan tingkat nyeri punggung yaitu 0,8 yang menunjukkan hubungan sangat kuat dan positif dan mempunyai tingkat signifikan pada level < 0,05 artinya sangat berhubungan. Hal ini menunjukkan ada pengaruh teknik gymball dengan penurunan tibgkat nyeri punggung.
Kata kunci : Tehnik Gym Ball, Penurunan nyeri punggung, ibu hamil trimester III