bluuebunny/crossref_metadata_2025_split huggingface dataset:
Matrix metalloproteinase-2 of human carotid atherosclerotic plaques promotes platelet activation
Authors: Lenti Massimo , Falcinelli Emanuela , Pompili Marcella , Rango Paola De , Conti Valentina , Guglielmini Giuseppe , Momi Stefania , Corazzi Teresa , Giordano Giuseppe , Gresele Paolo 
Published: February 2014

SummaryPurified active matrix metalloproteinase-2 (MMP-2) is able to promote platelet aggregation. We aimed to assess the role of MMP-2 expressed in atherosclerotic plaques in the platelet-activating potential of human carotid plaques and its correlation with ischaemic events. Carotid plaques from 81 patients undergoing endarterectomy were tested for pro-MMP-2 and TIMP-2 content by zymography and ELISA. Plaque extracts were incubated with gel-filtered platelets from healthy volunteers for 2 minutes before the addition of a subthreshold concentration of thrombin receptor activating peptide-6 (TRAP-6) and aggregation was assessed. Moreover, platelet deposition on plaque extracts immobilised on plastic coverslips under high shear-rate flow conditions was measured. Forty-three plaque extracts (53%) potentiated platelet aggregation (+233 ± 26.8%), an effect prevented by three different specific MMP-2 inhibitors (inhibitor II, TIMP-2, moAb anti-MMP-2). The pro-MMP-2/TIMP-2 ratio of plaques potentiating platelet aggregation was significantly higher than that of plaques not potentiating it (3.67 ± 1.21 vs 1.01 ± 0.43, p<0.05). Moreover, the platelet aggregation-potentiating effect, the active-MMP-2 content and the active MMP-2/pro-MMP-2 ratio of plaque extracts were significantly higher in plaques from patients who developed a subsequent major cardiovascular event. In conclusion, atherosclerotic plaques exert a prothrombotic effect by potentiating platelet activation due to their content of MMP-2; an elevated MMP-2 activity in plaques is associated with a higher rate of subsequent ischaemic cerebrovascular events.
PECAM-1-dependent heme oxygenase-1 regulation via an Nrf2-mediated pathway in endothelial cells
Authors: Saragih Hendry , Zilian Eva , Jaimes Yarúa , Paine Ananta , Figueiredo Constanca , Eiz-Vesper Britta , Blasczyk Rainer , Larmann Jan , Theilmeier Gregor , Burg-Roderfeld Monika , Andrei-Selmer Luminita-Cornelia , Becker Jan Ulrich , Santoso Sentot , Immenschuh Stephan 
Published: February 2014

SummaryThe antioxidant enzyme heme oxygenase (HO)-1, which catalyses the first and rate-limiting step of heme degradation, has major anti-inflammatory and immunomodulatory effects via its cell-type-specific functions in the endothelium. In the current study, we investigated whether the key endothelial adhesion and signalling receptor PECAM-1 (CD31) might be involved in the regulation of HO-1 gene expression in human endothelial cells (ECs). To this end PECAM-1 expression was down-regulated in human umbilical vein ECs (HUVECs) by an adenoviral vector-based knockdown approach. PECAM-1 knockdown markedly induced HO-1, but not the constitutive HO isoform HO-2. Nuclear translocation of the transcription factor NF-E2-related factor-2 (Nrf2), which is a master regulator of the inducible antioxidant cell response, and intracellular levels of reactive oxygen species (ROS) were increased in PECAM-1-deficient HUVECs, respectively. PECAM-1-dependent HO-1 regulation was also examined in PECAM-1 over-expressing Chinese hamster ovary and murine L-cells. Endogenous HO-1 gene expression and reporter gene activity of transiently transfected luciferase HO-1 promoter constructs with Nrf2 target sequences were decreased in PECAM-1 over-expressing cells. Moreover, a regulatory role of ROS for HO-1 regulation in these cells is demonstrated by studies with the antioxidant N-acetylcysteine and exogenous hydrogenperoxide. Finally, direct interaction of PECAM-1 with a native complex of its binding partner NB1 (CD177) and serine proteinase 3 (PR3) from human neutrophils, markedly induced HO-1 expression in HUVECs. Taken together, we demonstrate a functional link between HO-1 gene expression and PECAM-1 in human ECs, which might play a critical role in the regulation of inflammation.
Vertical Velocity and Physical Structure of Generating Cells and Convection in the Comma Head Region of Continental Winter Cyclones
Authors: Rosenow Andrew A. , Plummer David M. , Rauber Robert M. , McFarquhar Greg M. , Jewett Brian F. , Leon David 
Published: February 2014

Abstract
The vertical motion and physical structure of elevated convection and generating cells within the comma heads of three continental winter cyclones are investigated using the Wyoming W-band cloud radar mounted on the National Science Foundation/National Center for Atmospheric Research (NSF/NCAR) C-130, supplemented by analyses from the Rapid Update Cycle model and Weather Surveillance Radar-1988 Doppler (WSR-88D) data. The cyclones followed three distinct archetypical tracks and were typical of those producing winter weather in the midwestern United States. In two of the cyclones, dry air in the middle and upper troposphere behind the Pacific cold front intruded over moist Gulf of Mexico air at lower altitudes within the comma head, separating the comma head into two zones. Elevated convection in the southern zone extended from the cold-frontal surface to the tropopause. The stronger convective updrafts ranged from 2 to 7 m s−1 and downdrafts ranged from −2 to −6 m s−1. The horizontal scale of the convective cells was approximately 5 km. The poleward zone of the comma head was characterized by deep stratiform clouds topped by cloud-top generating cells that reached the tropopause. Updrafts and downdrafts within the generating cells ranged from 1 to 2 m s−1, with the horizontal scale of the cells from about 1 to 2 km. Precipitation on the poleward side of the comma head conformed to a seeder–feeder process—the generating cells seeding the stratiform cloud—which was forced by synoptic-scale ascent. In one case, shallow clouds behind the cyclone’s cold front were also topped by cloud-top generating cells, with vertical motions ranging from 1 to 2 m s−1.
An Update on the Oceanic Precipitation Rate and Its Zonal Distribution in Light of Advanced Observations from Space
Authors: Behrangi Ali , Stephens Graeme , Adler Robert F. , Huffman George J. , Lambrigtsen Bjorn , Lebsock Matthew 
Published: February 2014

Abstract
This study contributes to the estimation of the global mean and zonal distribution of oceanic precipitation rate using complementary information from advanced precipitation measuring sensors and provides an independent reference to assess current precipitation products. Precipitation estimates from the Tropical Rainfall Measuring Mission (TRMM) precipitation radar (PR) and CloudSat cloud profiling radar (CPR) were merged, as the two complementary sensors yield an unprecedented range of sensitivity to quantify rainfall from drizzle through the most intense rates. At higher latitudes, where TRMM PR does not exist, precipitation estimates from Aqua’s Advanced Microwave Scanning Radiometer for Earth Observing System (AMSR-E) complemented CloudSat CPR to capture intense precipitation rates. The high sensitivity of CPR allows estimation of snow rate, an important type of precipitation at high latitudes, not directly observed in current merged precipitation products. Using the merged precipitation estimate from the CloudSat, TRMM, and Aqua platforms (this estimate is abbreviated to MCTA), the authors’ estimate for 3-yr (2007–09) near-global (80°S–80°N) oceanic mean precipitation rate is ~2.94 mm day−1. This new estimate of mean global ocean precipitation is about 9% higher than that of the corresponding Climate Prediction Center (CPC) Merged Analysis of Precipitation (CMAP) value (2.68 mm day−1) and about 4% higher than that of the Global Precipitation Climatology Project (GPCP; 2.82 mm day−1). Furthermore, MCTA suggests distinct differences in the zonal distribution of precipitation rate from that depicted in GPCP and CMAP, especially in the Southern Hemisphere.
The Influence of Tropical Cyclone Size on Its Intensification
Authors: Carrasco Cristina Alexandra , Landsea Christopher William , Lin Yuh-Lang 
Published: February 2014

Abstract
This study investigates tropical cyclones of the past two decades (1990–2010) and the connection, if any, between their size and their ability to subsequently undergo rapid intensification (RI). Three different parameters are chosen to define the size of a tropical cyclone: radius of maximum wind (RMW), the average 34-knot (kt; 1 kt = 0.51 m s−1) radius (AR34), and the radius of the outermost closed isobar (ROCI). The data for this study, coming from the North Atlantic hurricane database second generation (HURDAT2), as well as the extended best-track dataset, are organized into 24-h intervals of either RI or slow intensification/constant intensity periods (non-RI periods). Each interval includes the intensity (maximum sustained surface wind speed), RMW, AR34, and ROCI at the beginning of the period and the change of intensity during the subsequent 24-h period. Results indicate that the ability to undergo RI shows significant sensitivity to initial size. Comparisons between RI and non-RI cyclones confirm that tropical cyclones that undergo RI are more likely to be smaller initially than those that do not. Analyses show that the RMW and AR34 have the strongest negative correlation with the change of intensity. Scatterplots imply there is a general maximum size threshold for RMW and AR34, above which RI is extremely rare. In contrast, the overall size of the tropical cyclones, as measured by ROCI, appears to have little to no relationship with subsequent intensification. The results of this work suggest that intensity forecasts and RI predictions in particular may be aided by the use of the initial size as measured by RMW and AR34.
Safety and Otoprotection of Metformin in Radiation‐Induced Sensorineural Hearing Loss in the Guinea Pig
Authors: Mujica‐Mota Mario A. , Salehi Pezhman , Devic Slobodan , Daniel Sam J. 
Published: February 2014

ObjectiveThere is currently no treatment available to prevent radiation‐induced sensorineural hearing loss. Metformin has antineoplastic effects and is able to regulate the mitochondrial production of reactive oxygen species after cellular stress, which is one of the mechanisms involved in apoptosis after radiation damage. The objective of this study was to determine the safety and radioprotective properties of metformin against radiation‐induced cochlear damage both in vitro and in vivo.Study DesignIn vitro and prospective animal study.SettingAnimal Care Facilities of the Montreal Children's Hospital Research Institute.MethodsCultured auditory hair cells (HEI‐OC1) were exposed to different concentrations of metformin to determine its safety. Cells were incubated with different metformin concentrations and subjected to radiation. Cell viability after experiments was determined with the 3‐(4,5‐dimethylthiazol‐2‐yl)‐5‐(3‐carboxymethoxyphenyl)‐2‐(4‐sulfophenyl)‐2H‐tetrazolium (MTS) assay. Sixteen guinea pigs were divided in 2 groups: drinking tap water (n = 8) and drinking water containing metformin (n = 8). The animals were unilaterally irradiated for 20 days (total dose 70 Gy), and the ears were divided in 4 groups: control (n = 8), irradiated (n = 8), metformin (n = 8), and experimental (n = 8). Auditory brainstem responses were assessed before and 1, 6, and 16 weeks after completion of radiotherapy.ResultsMetformin was not cytotoxic or radioprotective in cultured auditory hair cells. Experimental ears had less hearing loss than radiated ones; however, differences were not statistically significant (P >. 05).ConclusionMetformin is not ototoxic or radioprotective in vitro or in vivo. Ears solely subjected to metformin had better hearing thresholds than the rest of the groups.
The disparate management of superficial venous thrombosis in primary and secondary care
Authors: Lane Tristan RA , Sritharan Kaji , Herbert J Rosalind , Franklin Ian J , Davies Alun H 
Published: February 2014

Objectives Superficial venous thrombosis is common and traditionally considered a benign condition requiring only symptomatic treatment. Recent evidence, however, advocates more aggressive management. Extensive guidance is available but actual practice is unknown. This study aimed to assess the management of superficial venous thrombosis by general practitioners (primary care physicians) and vascular surgeons. Methods A 19-question validated electronic survey was created and circulated by e-mail to general practitioners and vascular surgeons in the United Kingdom. The survey evaluated presentation, investigation and treatment of superficial venous thrombosis. Results Three hundred sixty-nine surveys were returned from 197 vascular surgeons and 172 general practitioners. Most clinicians saw less than 20 cases a year, with 40% of clinicians not performing any investigations. Venous duplex was the investigation of choice in over 55%. Treatment with anti-inflammatory drugs was widespread, but anticoagulation and compression were seldom prescribed. Follow-up and treatment duration were disparate. Discussion The management of superficial venous thrombosis varies widely despite good levels of evidence and guidance. Investigation and treatment of superficial venous thrombosis show marked differences both between and within groups. Improvements in education are required to optimise the treatment pathway and advance patient care.
Effects of sociodemographic factors and maternal anxiety on preoperative anxiety in children
Authors: Cagiran Esra , Sergin Demet , Deniz Mustafa Nuri , Tanattı Burçak , Emiroglu Neslihan , Alper Isik 
Published: February 2014

Objective To investigate the effects of sociodemographic factors and maternal anxiety levels on behaviour in children undergoing surgery. Methods This study included children aged 3–12 years who were scheduled for surgery, and their respective mothers. Each mother completed a questionnaire concerning sociodemographic and economic characteristics. Maternal anxiety was assessed using the State-Trait Anxiety Inventory (STAI) form Tx-1, following transfer of the child to the operating room. An anaesthesiologist rated preoperative anxiety in each child using the Frankl Behaviour Rating Scale (FBRS) and Venham Picture Test (VPT). Results One hundred children (mean ± SD age 7 ± 2.7 years) who received dental (47%), plastic (39%) or urological (14%) surgery, and 100 mothers (mean ± SD age 34 ± 6.6 years) were included. A statistically significant difference between maternal STAI Tx-1 scores and children’s VPT scores was revealed. There was no significant difference between maternal STAI Tx-1 scores and children’s FBRS scores. Maternal education level and socioeconomic status had no effect on FBRS, VPT and STAI Tx-1 scores. Conclusion Maternal knowledge and experience of anaesthesia, and high levels of maternal anxiety, may be related to increased anxiety in children undergoing surgery.
Acetaminophen intoxication is associated with decreased serum paraoxonase and arylesterase activities and increased lipid hydroperoxide levels
Authors: Karadas S , Aslan M , Gonullu H , Kati C , Duran L , Olmez S , Kucukoglu ME , Demir H 
Published: February 2014

Background: Acetaminophen is at present one of the most commonly used analgesics and antipyretics. Recent evidence has suggested that oxidative stress is involved in the mechanism of acetaminophen intoxication. Paraoxonase-1 (PON1) plays an important role as an endogenous free-radical scavenging molecule. The aim of this study was to evaluate the influence of serum PON1 activity and oxidative stress in patients with acetaminophen intoxication. Methods: A total of 20 patients with acetaminophen intoxication and 25 healthy controls were enrolled. Serum total antioxidant capacity (TAC), lipid hydroperoxide (LOOH) levels, and paraoxonase and arylesterase activities were measured spectrophotometrically. Results: The serum TAC levels and the paraoxonase and arylesterase activities were significantly lower in patients with acetaminophen intoxication compared with controls (all, p < 0.001), while the serum LOOH levels were significantly higher ( p < 0.001). Conclusions: Our results suggest that decreased PON1 activity seems to be associated with increased oxidative stress in patients with acetaminophen intoxication. Measuring serum PON1 activity may be useful in assessing the development of toxicity risk in acetaminophen toxicity. It would be useful to recommend vitamins with antioxidant effects such as vitamins C and E along with medical treatments.
Strengthening practical wisdom
Authors: Eriksen Kristin Ådnøy , Dahl Hellen , Karlsson Bengt , Arman Maria 
Published: February 2014

Background: Practical wisdom, understood as knowing how to be or act in any present situation with clients, is believed to be an essential part of the knowledge needed to be a professional mental health worker. Exploring processes of adapting, extending knowledge and refining tacit knowledge grounded in mental health workers’ experiences with being in practice may bring awareness of how mental health workers reflect, learn and practice professional ‘artistry’. Research question: The aim of the article was to explore mental health workers’ processes of development and learning as they appeared in focus groups intended to develop practical wisdom. The main research question was ‘How might the processes of development and learning contribute to developing practical wisdom in the individual as well as in the practice culture?’ Research design: The design was multi-stage focus groups, and the same participants met four times. A phenomenological hermeneutical method for researching lived experience guided the analysis. Participants and context: Eight experienced mental health workers representing four Norwegian municipalities participated. The research context was community-based mental health services. Ethical considerations: The study was reported to Norwegian Social Data Services, and procedures for informed consent were followed. Findings: Two examples of processes of re-evaluation of experience (Association, Integration, Validation, Appropriation and Outcomes and action) were explored. The health workers had developed knowledge in previous encounters with clients. In sharing practice experiences, this knowledge was expressed and developed, and also tested and validated against the aims of practice. Discussions led to adapted and extended knowledge, and as tacit knowledge was expressed it could be used actively. Discussion: Learning to reflect, being ready to be provoked and learning to endure indecisiveness may be foundational in developing practical wisdom. Openness is demanding, and changing habits of mind is difficult. Conclusion: Reflection on, and confrontation with, set practices are essential to building practice cultures in line with the aims of mental health services.
Suicide around public holidays
Authors: Barker Emma , O’Gorman John , De Leo Diego 
Published: February 2014

Objective: To examine the frequency of suicides on holidays and special days of the year, specifically in Queensland, Australia. Methods: We analysed data from the Queensland Suicide Register between 1990 and 2009. The days examined were: Easter, Christmas, New Year, Valentine’s Day and Anzac Day. We compared suicide cases on these days with the average counts for periods before and after the days. Results: There was a statistically significant increase in suicides on Christmas Eve and on New Year’s Day. Our results are discussed in light of trends reported in the literature. Conclusion: The beginning and end of the festive season are times when special attention is warranted for those with a heightened risk of suicide.
Developing performance indicators for homeless outreach psychiatric services
Authors: Holmes Alexander , Bradley Gail , Carslie Trevor , Lhuede Kate 
Published: February 2014

Objective: Performance indicators (PIs) aim to improve services by measuring key activities in a way that allows comparison over time, between services and against benchmarks. This paper describes the development and implementation of Homeless Psychiatric Service PIs and explores their potential benefits and limitations. Method: We collected descripton of quality service from key stakeholders. We identified eight key parameters, from which PIs were developed and tested over a 12-month period. Results: The use of the PIs led to increased awareness of the practice being measured. PIs were used to stimulate practice changes. They played a positive role in team dynamics and were useful in clarifying team aims and identity. The main challenge to their use was the burden of data collection and analysis. Conclusion: Homeless service PIs can assist in determining how well the programs are performing in activities that are relevant to clients and non clinical services for the homeless. With the movement of homeless clients away from inner urban areas, homeless performance measures may aid teams to develop the capacity to work effectively with homeless clients.
Electoral Career Movements and the Flow of Political Power in the American Federal System
Authors: Squire Peverill 
Published: February 2014

AbstractI offer a novel way of documenting the flow of power between the state and federal governments. Rather than look at programs or expenditures, I examine the behavior of elected officials. Assuming that ambitious politicians gravitate toward the locus of political power, I track the flow of elected officials in the American federal system. Specifically, I look at the career paths of more than 12,000 individuals who served in the U.S. Congress between 1789 and 2012. By analyzing the movement of elected officials between the state and federal levels, I confirm the prevailing story line on the evolution of American federalism while generating a measure that can be used to assess the relative distribution of power in the system across time.
Mixed messages
Authors: Rosenthal Meagen , Chen Christopher B. , Hall Kevin , Tsuyuki Ross T. 
Published: February 2014

Background: More than 5 years ago, the Blueprint for Pharmacy developed a plan for transitioning pharmacy practice toward more patient-centred care. Much of the strategy for change involves communicating the new vision. Objective: To evaluate the communication of the Vision for Pharmacy by the organizations and corporations that signed the Blueprint for Pharmacy’s Commitment to Act. Methods: The list of 88 signatories of the Commitment to Act was obtained from the Blueprint for Pharmacy document. The website of each of these signatories was searched for all references to the Blueprint for Pharmacy or Vision for Pharmacy. Each of the identified references was then analyzed using summative content analysis. Results: A total of 934 references were identified from the webpages of the 88 signatories. Of these references, 549 were merely links to the Blueprint for Pharmacy’s website, 350 of the references provided some detailed information about the Blueprint for Pharmacy and only 35 references provided any specific plans to transition pharmacy practice. Conclusion: Widespread proliferation of the Vision for Pharmacy has not been achieved. One possible explanation for this is that communication of the vision by the signatories has been incomplete. To ensure the success of future communications, change leaders must develop strategies that consider how individual pharmacists and pharmacies understand the message.
The type I BMP receptor Alk3 is required for the induction of hepatic hepcidin gene expression by interleukin-6
Authors: Mayeur Claire , Lohmeyer Lisa K. , Leyton Patricio , Kao Sonya M. , Pappas Alexandra E. , Kolodziej Starsha A. , Spagnolli Ester , Yu Binglan , Galdos Rita L. , Yu Paul B. , Peterson Randall T. , Bloch Donald B. , Bloch Kenneth D. , Steinbicker Andrea U. 
Published: February 2014

Key Points
Presence of the BMP type I receptor Alk3 is required for interleukin-6 to induce hepatic hepcidin gene expression. Alk3 contributes to the induction of hypoferremia by interleukin-6.