bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differences between Faster versus Slower Components of Convectively Coupled Equatorial Waves
Authors: Yasunaga Kazuaki , Mapes Brian 
Published: September 2013

Abstract
This paper describes an analysis of multiyear satellite datasets that subdivide two halves (faster and slower) of the space–time spectral signal peaks corresponding to convectively coupled equatorial waves such as Kelvin and inertia–gravity waves [n = 0 eastward inertia–gravity wave (EIGn0 wave), and n = 1 and n = 2 westward inertia–gravity waves (WIGn1 and WIGn2 waves, respectively)]. The faster (slower) component of an equatorial wave is defined as that which has a spectral signal peak in the regions with deeper (shallower) equivalent depths. The data obtained from the Tropical Rainfall Measuring Mission (TRMM) precipitation radar (TRMM-PR) are composited around space–time-filtered equatorial-belt data from the TRMM-3B42 rainfall product to separately estimate the convective and stratiform rainfall modulations.
Results indicate that the faster components of WIGn1 and WIGn2 waves modulate convective rain relatively more (and stratiform rain relatively less) than their slower counterparts. For Kelvin and EIGn0 waves, however, there is no significant difference in the rainfall modulation between their faster and slower components. A space–time cospectral analysis of the satellite-retrieved rainfall and moisture shows that in the spectral regions corresponding to WIGn1 and WIGn2 waves, precipitation is significantly correlated with low-level moisture but not with midlevel moisture. In contrast, significant coherence between rainfall and moisture at these levels is found in the spectral regions corresponding to the Kelvin and EIGn0 waves. These results may bear on different convection–wave coupling mechanisms for these “divergent” waves (stratiform instability versus moisture–stratiform instability).
Assimilation of Time-Averaged Pseudoproxies for Climate Reconstruction
Authors: Steiger Nathan J. , Hakim Gregory J. , Steig Eric J. , Battisti David S. , Roe Gerard H. 
Published: September 2013

Abstract
The efficacy of a novel ensemble data assimilation (DA) technique is examined in the climate field reconstruction (CFR) of surface temperature. A minimalistic, computationally inexpensive DA technique is employed that requires only a static ensemble of climatologically plausible states. Pseudoproxy experiments are performed with both general circulation model (GCM) and Twentieth Century Reanalysis (20CR) data by reconstructing surface temperature fields from a sparse network of noisy pseudoproxies. The DA approach is compared to a conventional CFR approach based on principal component analysis (PCA) for experiments on global domains. DA outperforms PCA in reconstructing global-mean temperature in all experiments and is more consistent across experiments, with a range of time series correlations of 0.69–0.94 compared to 0.19–0.87 for the PCA method. DA improvements are even more evident in spatial reconstruction skill, especially in sparsely sampled pseudoproxy regions and for 20CR experiments. It is hypothesized that DA improves spatial reconstructions because it relies on coherent, spatially local temperature patterns, which remain robust even when glacial states are used to reconstruct nonglacial states and vice versa. These local relationships, as utilized by DA, appear to be more robust than the orthogonal patterns of variability utilized by PCA. Comparing results for GCM and 20CR data indicates that pseudoproxy experiments that rely solely on GCM data may give a false impression of reconstruction skill.
Strong Westerly Wind Events in the Strait of Juan de Fuca
Authors: Mass Clifford F. , Warner Michael D. , Steed Rick 
Published: September 2013

Abstract
Strong westerly wind events occur regularly within and downstream of the Strait of Juan de Fuca of Washington State. During such strong gap wind periods, flow can accelerate to 30–50 kt (1 kt = 0.51 m s−1), with gusts sometimes reaching 80 kt or more. Strong winds in the strait and downstream of its eastern exit have caused substantial damage and loss of life. Strait of Juan de Fuca westerly wind events are often associated with sharp short-wave troughs coming out of the northwest, strong lower-tropospheric northwesterly geostrophic flow roughly paralleling the axis of the strait, and a large along-strait pressure gradient near the surface. Strong westerly flow in the strait also accompanies the passage of intense low pressure centers across northwest Washington State and southern British Columbia. The climatology of westerly wind events in the strait is presented, as well as their composite synoptic evolution. Using both mesoscale observations and high-resolution numerical simulations, it is shown that modern modeling systems can realistically simulate the mesoscale evolution of strait wind events. It is shown that strait gap wind events are associated with downward mixing of strong gap-parallel geostrophic winds in the lower troposphere and acceleration down a low-level pressure gradient produced by the passage of a synoptic trough and terrain-induced pressure perturbations.
Obstructive Sleep Apnea: Strategies for Minimizing Liability and Enhancing Patient Safety
Authors: Svider Peter F. , Pashkova Anna A. , Folbe Adam J. , Eloy Jean Daniel , Setzen Michael , Baredes Soly , Eloy Jean Anderson 
Published: September 2013

ObjectiveTo characterize malpractice litigation regarding obstructive sleep apnea (OSA) and educate physicians on frequently cited factors.Study Design and SettingAnalysis of the Westlaw legal databaseMethodsJury verdict and settlement reports were examined for outcome, awards, patient demographic factors, defendant specialty, and alleged causes of malpracticeResultsOut of 54 identified cases, 33 (61.1%) cases were resolved in favor of defendants, 12 (22.2%) via settlement, and 9 (16.7%) through jury award. Median settlement and jury awards did not significantly differ ($750,000 vs $550,000, P >. 50). Age and gender did not affect outcome. Otolaryngologists and anesthesiologists were the most frequently named defendants. Forty‐seven cases (87.1%) stemmed from OSA patients who underwent procedures with resultant perioperative adverse events. Common alleged factors included death (48.1%), permanent deficits (42.6%), intraoperative complications (35.2%), requiring additional surgery (25.9%), anoxic brain injury (24.1%), inadequate informed consent (24.1%), inappropriate medication administration (22.2%), and inadequate monitoring (20.4%).ConclusionLitigation related to OSA is frequently associated with perioperative complications more than nonoperative issues such as a failure to diagnose this disorder. Nonetheless, OSA is considerably underdiagnosed, and special attention should be paid to at‐risk patients, including close monitoring of their clinical status and the medications they receive. For patients with diagnosed or suspected OSA with planned operative intervention, whether for OSA or an unrelated issue, a comprehensive informed consent process detailing the factors outlined in this analysis is an effective strategy to increase communication and improve the physician‐patient relationship, minimize liability, and ultimately improve patient safety.
Differential regulation of cell functions by CSD peptide subdomains
Authors: Reese Charles , Dyer Shanice , Perry Beth , Bonner Michael , Oates James , Hofbauer Ann , Sessa William , Bernatchez Pascal , Visconti Richard P , Zhang Jing , Hatfield Corey M , Silver Richard M , Hoffman Stanley , Tourkina Elena 
Published: September 2013

Abstract

Background
In fibrotic lung diseases, expression of caveolin-1 is decreased in fibroblasts and monocytes. The effects of this deficiency are reversed by treating cells or animals with the caveolin-1 scaffolding domain peptide (CSD, amino acids 82–101 of caveolin-1) which compensates for the lack of caveolin-1. Here we compare the function of CSD subdomains (Cav-A, Cav-B, Cav-C, Cav-AB, and Cav-BC) and mutated versions of CSD (F92A and T90A/T91A/F92A).

Methods
Migration toward the chemokine CXCL12 and the associated expression of F-actin, CXCR4, and pSmad 2/3 were studied in monocytes from healthy donors and SSc patients. Fibrocyte differentiation was studied using PBMC from healthy donors and SSc patients. Collagen I secretion and signaling were studied in fibroblasts derived from the lung tissue of healthy subjects and SSc patients.

Results
Cav-BC and CSD at concentrations as low as 0.01 μM inhibited the hypermigration of SSc monocytes and TGFβ-activated Normal monocytes and the differentiation into fibrocytes of SSc and Normal monocytes. While CSD also inhibited the migration of poorly migrating Normal monocytes, Cav-A (and other subdomains to a lesser extent) promoted the migration of Normal monocytes while inhibiting the hypermigration of TGFβ-activated Normal monocytes. The effects of versions of CSD on migration may be mediated in part via their effects on CXCR4, F-actin, and pSmad 2/3 expression. Cav-BC was as effective as CSD in inhibiting fibroblast collagen I and ASMA expression and MEK/ERK signaling. Cav-C and Cav-AB also inhibited collagen I expression, but in many cases did not affect ASMA or MEK/ERK. Cav-A increased collagen I expression in scleroderma lung fibroblasts. Full effects on fibroblasts of versions of CSD required 5 μM peptide.

Conclusions
Cav-BC retains most of the anti-fibrotic functions of CSD; Cav-A exhibits certain pro-fibrotic functions. Results obtained with subdomains and mutated versions of CSD further suggest that the critical functional residues in CSD depend on the cell type and readout being studied. Monocytes may be more sensitive to versions of CSD than fibroblasts and endothelial cells because the baseline level of caveolin-1 in monocytes is much lower than in these other cell types.
Changes to mitochondrial ultrastructure in optic nerve vulnerable to secondary degeneration in vivo are limited by irradiation at 670 nm
Authors: Cummins Nadia , Bartlett Carole A , Archer Michael , Bartlett Elora , Hemmi Jan M , Harvey Alan R , Dunlop Sarah A , Fitzgerald Melinda 
Published: September 2013

Abstract

Background
Traumatic injury to the central nervous system results in damage to tissue beyond the primary injury, termed secondary degeneration. Key events thought to be associated with secondary degeneration involve aspects of mitochondrial function which may be modulated by red/near-infrared irradiation therapy (R/NIR-IT), but precisely how mitochondria are affected in vivo has not been investigated. Secondary degeneration was modelled by transecting the dorsal aspect of the optic nerve in adult rats and mitochondrial ultrastructure in intact ventral optic nerve vulnerable to secondary degeneration investigated with transmission electron microscopy.

Results
Despite reported increases in fission following central nervous system injury, we saw no change in mitochondrial densities in optic nerve vulnerable to secondary degeneration in vivo. However, in axons, frequency distributions of mitochondrial profile areas showed higher cumulative probabilities of smaller mitochondrial profiles at day 1 after injury. Glial mitochondrial profiles did not exhibit changes in area, but a more elliptical mitochondrial shape was observed at both day 1 and 7 following injury. Importantly, mitochondrial autophagic profiles were observed at days 1 and 7 in optic nerve vulnerable to secondary degeneration in vivo. Citrate synthase activity was used as an additional measure of mitochondrial mass in ventral optic nerve and was decreased at day 7, whereas mitochondrial aconitase activity increased at day 1 and day 28 after injury in optic nerve vulnerable to secondary degeneration. R/NIR-IT has been used to treat the injured central nervous system, with reported improvements in oxidative metabolism suggesting mitochondrial involvement, but ultrastructural information is lacking. Here we show that R/NIR-IT of injured animals resulted in distributions of mitochondrial areas and shape not significantly different from control and significantly reduced mitochondrial autophagic profiles. R/NIR-IT also resulted in decreased citrate synthase activity (day 7) and increased aconitase activity (day 1) in optic nerve vulnerable to secondary degeneration.

Conclusions
These findings suggest that mitochondrial structure and activity of enzymes of the citric acid cycle are dynamically altered during secondary degeneration in vivo and R/NIR-IT may protect mitochondrial structure.
Molecular cloning and characterisation of SlAGOfamily in tomato
Authors: Xian Zhiqiang , Yang Yingwu , Huang Wei , Tang Ning , Wang Xinyu , Li Zhengguo 
Published: September 2013

Abstract

Background
AGO (Argonaute) protein participates in plant developmental processes and virus defense as a core element of transcriptional regulator or/and post-transcriptional regulator in RNA induced silencing complex (RISC), which is guided by small RNAs to repress target genes expression. Previously, it was revealed that 15 putative AGO genes in tomato genome.

Results
In present study, out of 15 detected SlAGO genes, only SlAGO4C and SlAGO15 couldn’t be detected in roots, stems, leaves, buds, flowers and fruit of tomato by 30 cycles of PCR. SlAGO7 could be detected in early stage of fruit (-2 dpa, 0 dpa and 4 dpa), but it was significantly down-regulated in fruit collected on the 6 days post anthesis. Moreover, SlAGO5 could only be detected in reproductive tissues and SlAGO4D was specifically detected in fruit. According to blast result with miRNA database, three SlAGO genes harbored complementary sequences to miR168 (SlAGO1A and SlAGO1B) or miR403 (SlAGO2A). 5′ RACE (Rapid amplification of cDNA ends) mapping was used to detect the 3′ cleavage products of SlAGO mRNAs. In addition, subcellular localization of SlAGO proteins was detected. Our results showed that most SlAGO proteins localized to nucleus and cytoplasm. Importantly, nuclear membrane localization of AGO proteins was observed. Furthermore, mutated miR168 complementary site of SlAGO1A resulted in expanded localization of SlAGO1A, indicating that miR168 regulated localization of SlAGO1A.

Conclusions
Our results contribute to demonstration of potential roles of these newly isolated AGO family in tomato developmental processes and proved the conserved relationships between AGO genes and miRNAs in tomato, which might play important roles in tomato development and virus defense.
Comparison of 9-month angiographic outcomes of Resolute zotarolimus-eluting and everolimus-eluting stents in a real world setting of coronary intervention in Korea
Authors: Lee Joo Myung , Youn Tae-Jin , Park Jin Joo , Oh Il-Young , Yoon Chang-Hwan , Suh Jung-Won , Cho Young-Seok , Cho Goo-Yeong , Chae In-Ho , Choi Dong-Ju 
Published: September 2013

AbstractBackgroundClinical outcomes of new-generation drug-eluting stents (DES), Everolimus-eluting stent (EES) or Resolute zotarolimus-eluting stent (R-ZES), have been reported. However, angiographic follow-up data of new-generation DES are limited, especially in Asians. We investigated the angiographic and clinical outcomes of EES and R-ZES in a real-world setting of Korean patients.MethodsAngiographic and clinical outcomes of 679 patients (866 lesions) who had been treated with EES or R-ZES from Jun 2008 to May 2010 were evaluated. The primary analysis was to compare in-segment late loss at 9 months and the secondary analyses were to compare the clinical outcomes.ResultsIn-segment late loss at 9-month follow-up angiography was 0.23 ± 0.52 mm for EES and 0.29 ± 0.64 mm for R-ZES (p = 0.248). In addition, the rate of binary restenosis did not show between-group differences (5.8%vs.6.8% for EES and R-ZES, respectively, p = 0.716). During a median follow-up of 33 months, there were no significant differences in Kaplan-Meier estimates of target lesion failure (TLF) (7.5% vs. 7.9% for EES and R-ZES, respectively, p = 0.578) and patient-oriented composite outcomes (POCO including all-cause death, any myocardial infarction, and any revascularization, 22.8% vs. 20.1%, p = 0.888). The adjusted hazard ratios for TLF and POCO were 0.875 (95% CI 0.427 - 1.793; p = 0.715) and 1.029 (95% CI 0.642 - 1.650; p = 0.904), respectively, for EES over R-ZES in the propensity score matched group analysis.ConclusionsIn Korean patients undergoing new-generation DES implantation for coronary artery disease, EES and R-ZES showed similar angiographic outcomes at 9 months and comparable clinical outcomes during 2.8 years of median follow-up.
Molecular characterization of T cell receptor beta variable in the peripheral blood T cell repertoire in subjects with active tuberculosis or latent tuberculosis infection
Authors: Yang Jiezuan , He Jianqin , Huang Haijun , Ji Zhongkang , Wei Li , Ye Ping , Xu Kaijin , Li Lanjuan 
Published: September 2013

Abstract

Background
T cells are closely linked to the clinical manifestations of subjects with Mycobacterium tuberculosis (MTB) infection. T cell receptor beta variable (TCRBV) is a signal and indicative molecule on the membrane of T lymphocytes, reflecting the composition and specificity of T cells. The molecular profiles of TCRBV in peripheral blood mononuclear cells (PBMCs) and their subpopulations (CD4+ and CD8+ T cells) from subjects with active tuberculosis (TB) or latent TB infection (LTBI) have not been well described.

Methods
In 42 subjects with active TB or LTBI, PMBCs and their subsets were separated and sorted. The molecular profiles of the TCRBV complementarity determining region 3 (CDR3) in the three cell populations were investigated using our recently developed gene melting spectral pattern (GMSP) assay. The TCRBV members were then cloned and sequenced when their GMSP image profiles showed a single-peak.

Results
The average number of skewed TCRBV molecules in the CD4+ cell subset was significantly higher than that in PBMCs and CD8+ T cells. TCRBV12, BV13.1, BV13.2, and BV24 were expressed more prevalently than other TCRBV gene families in the three cell populations. In addition, relatively conserved amino acid motifs were identified in TCRBV5.1 and BV20 CDR3 in PBMCs and its subsets. The monoclonal TCRBV14 and BV23 expressed were different between active TB and LTBI subjects.

Conclusions
These results indicate that the T cell immune response is complex and multi-specific in active TB and LTBI subjects. Analysis of TCRBV expression in CD4+ T cells suggest that it could be useful in assessing the composition and status of circulating T cells. Furthermore, the expression of TCRBV14, BV23 and the sequencing of CDR3 amino acid motifs of TCRBV5.1, BV20 could be used in the differential diagnosis and treatment of subjects with active TB or LTBI.
Clinical utility of gray scale renal ultrasound in acute kidney injury
Authors: Podoll Amber , Walther Carl , Finkel Kevin 
Published: September 2013

Abstract

Background
Acute kidney injury occurs commonly in hospitalized patients and is associated with significant morbidity and mortality. Although renal ultrasound is often performed, its clinical utility in determining of the cause of acute kidney injury, particularly the detection of urinary tract obstruction, is not established.

Methods
Retrospective cohort study of all adult inpatients that underwent renal ultrasound for acute kidney injury over a 3-year period at a large university teaching hospital. The frequency of renal ultrasound abnormalities and clinical characteristics that predicted the finding of urinary tract obstruction was determined.

Results
Over the 3-year period, 1471 renal ultrasounds were performed of which 55% (810) were for evaluation of acute kidney injury. Renal ultrasound was normal in 62% (500 of 810) of patients. Hydronephrosis was detected in only 5% (42 of 810) of studies and in only 2.3% (19 of 810) of the cases was obstructive uropathy considered the cause of acute kidney injury. The majority of these patients (14 of 19) had a medical history suggestive of urinary tract obstruction. Less than 1% of patients (5 of 810) had urinary tract obstruction on ultrasound without a suggestive medical history. Most other ultrasound findings were incidental and did not establish an etiology for the acute kidney injury.

Conclusions
Renal ultrasound for evaluation of acute kidney injury is indicated if there is medical history suggestive of urinary tract obstruction. Otherwise, renal ultrasound is unlikely to yield useful results and should be used more selectively based on patients’ medical history.
Molecular analysis of a consanguineous Iranian polycystic kidney disease family identifies a PKD2mutation that aids diagnostics
Authors: Vazifehmand Reza , Rossetti Sandro , Saber Sassan , Khorshid Hamid Reza Khorram , Harris Peter C 
Published: September 2013

Abstract

Background
Polycystic kidney diseases (PKD) are a group of monogenic disorders that are inherited dominantly (autosomal dominant PKD; ADPKD) or recessively, including, autosomal recessive PKD (ARPKD). A number of recessive, syndromic disorders also involve PKD but have a range of pleiotropic phenotypes beyond the kidney, and are enriched in consanguineous families.

Case presentation
We describe here a consanguineous Iranian pedigree in which PKD was diagnosed in four generations, but also included cases with additional abnormalities, including mental retardation. We employed molecular screening to reveal the etiology of the PKD. Since the PKD seemed to be dominantly inherited, molecular diagnostics was performed by direct sequencing of the ADPKD genes, PKD1 and PKD2. Clinical and imaging data was collected on family members. The sequence analysis revealed a PKD2 single base-pair deletion, c.1142delG, and segregation was demonstrated in 16 PKD patients from different branches of the family. In keeping with other reports, the PKD2 phenotype in this family was overall mild, and characterized by conserved kidney function, although 12 cases had some evidence of renal insufficiency. Several younger mutation carriers had borderline or no clinical characteristics of ADPKD, while a patient that required a renal transplant at 14 y did not have the PKD2 mutation.

Conclusions
The molecular analysis of an Iranian family showed that the PKD was due to a PKD2 mutation. The identification of the causative mutation allowed an accurate diagnosis in a number of individuals with equivocal imaging data. Consequently, these patients could be followed appropriately as at-risk individuals. In addition, the PKD2 diagnosis ruled out a syndromic form of PKD as the cause of the additional phenotypes in the family.