bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does Hyphal‐Tip Ensure the Same Allelic Composition at SSR Loci as Monosporic Isolates of Sclerotinia sclerotiorum?
Authors: Lehner Miller S. , Paula Júnior Trazilbo J. , Mizubuti Eduardo S. G. 
Published: July 2015

AbstractThe proper characterization of individual is a basic stage in population genetic studies. In Sclerotinia sclerotiorum, genetic uniformity of an individual can be obtained by isolation of single ascospore; however, hyphal‐tip isolates are commonly used in genetic studies. The aim of this study was to assess whether hyphal‐tip isolates of S. sclerotiorum can be used as surrogate of monoascosporic (monosporic) isolates. Twenty‐eight isolates of S. sclerotiorum were collected from common bean plants with white mold symptoms and were purified by hyphal‐tip or single ascospore. The correspondence between hyphal‐tip and monosporic isolates was assessed through the allelic composition at 10 microsatellite (SSR) loci of the isolates obtained by both methods. For the SSR loci comprised of dinucleotide repeats in 92% of the cases, the difference (di) between the amplicon size values for hyphal‐tip and monosporic isolates was no more than one base pair. For the loci comprised of tetra or pentanucleotide repeats in 89% of the cases, di was no more than one base pair. The same allelic profile was found for hyphal‐tip or single ascospore isolates of S. sclerotiorum. When monosporic isolates cannot be easily obtained, hyphal‐tip can safeguard the genotypic identity of S. sclerotiorum isolates.
Pleasant Events, Hopelessness, and Quality of Life in Rural Older Adults
Authors: Scogin Forrest , Morthland Martin , DiNapoli Elizabeth A. , LaRocca Michael , Chaplin William 
Published: July 2015

AbstractPurposeRural older adults are susceptible to depression and reduced quality of life. This study explored contrasting explanations (behavioral vs cognitive route) for the relation of emotional distress with quality of life.MethodsThis retrospective study included rural older adults (N = 134) with reduced quality of life and increased psychological symptoms. Multiple mediation analysis was conducted to test the indirect effect of engagement in pleasant events and hopelessness on the emotional distress and quality of life relation.FindingsBoth engagement in pleasant events and hopelessness were found to partially mediate the relation between emotional distress and quality of life.ConclusionsTargeting both hopelessness and engagement in pleasant events may be helpful in improving the quality of life of vulnerable, rural older adults.
Heinrich Schenker and the Radio
Authors: HEWLETT KIRSTIE 
Published: July 2015

ABSTRACTHeinrich Schenker had a radio installed in his home on 19 October 1924, less than three weeks after the inception of broadcasting in Austria. Almost overnight, it became his main connection to cultural life in Vienna: from the day his receiver was installed until his death in January 1935, he documented references to over 1,000 broadcasts of concerts, plays and talks in his diary, touching on anything from orchestral concerts, opera and jazz bands to plays and talks. In contrast to the portrayal – and self‐portrayal – of Schenker as a misanthrope, utterly disillusioned by the culture of his time, his extensive documentation of his listening habits offers a rare glimpse into the true breadth of his cultural interests in private. This article traces the role that radio assumed in his life, following its transition from a resource that radically transformed his access to the arts to a source of diversion in his final years.
Gene duplication and divergence affecting drug content in Cannabis sativa
Authors: Weiblen George D. , Wenger Jonathan P. , Craft Kathleen J. , ElSohly Mahmoud A. , Mehmedic Zlatko , Treiber Erin L. , Marks M. David 
Published: July 2015

Summary

Cannabis sativa is an economically important source of durable fibers, nutritious seeds, and psychoactive drugs but few economic plants are so poorly understood genetically.

Marijuana and hemp were crossed to evaluate competing models of cannabinoid inheritance and to explain the predominance of tetrahydrocannabinolic acid (THCA) in marijuana compared with cannabidiolic acid (CBDA) in hemp. Individuals in the resulting F2 population were assessed for differential expression of cannabinoid synthase genes and were used in linkage mapping. Genetic markers associated with divergent cannabinoid phenotypes were identified.

Although phenotypic segregation and a major quantitative trait locus (QTL) for the THCA/CBDA ratio were consistent with a simple model of codominant alleles at a single locus, the diversity of THCA and CBDA synthase sequences observed in the mapping population, the position of enzyme coding loci on the map, and patterns of expression suggest multiple linked loci. Phylogenetic analysis further suggests a history of duplication and divergence affecting drug content.

Marijuana is distinguished from hemp by a nonfunctional CBDA synthase that appears to have been positively selected to enhance psychoactivity. An unlinked QTL for cannabinoid quantity may also have played a role in the recent escalation of drug potency.
Cyanotic congenital heart disease (CCHD): focus on hypoxemia, secondary erythrocytosis, and coagulation alterations
Authors: Zabala Luis M. , Guzzetta Nina A. 
Published: July 2015

SummaryChildren with cyanotic congenital heart disease (CCHD) have complex alterations in their whole blood composition and coagulation profile due to long‐standing hypoxemia. Secondary erythrocytosis is an associated physiological response intended to increase circulating red blood cells and oxygen carrying capacity. However, this response is frequently offset by an increase in whole blood viscosity that paradoxically reduces blood flow and tissue perfusion. In addition, the accompanying reduction in plasma volume leads to significant deficiencies in multiple coagulation proteins including platelets, fibrinogen and other clotting factors. On the one hand, these patients may suffer from severe hyperviscosity and subclinical ‘sludging’ in the peripheral vasculature with an increased risk of thrombosis. On the other hand, they are at an increased risk for postoperative hemorrhage due to a complex derangement in their hemostatic profile. Anesthesiologists caring for children with CCHD and secondary erythrocytosis need to understand the pathophysiology of these alterations and be aware of available strategies that lessen the risk of bleeding and/or thrombosis. The aim of this review is to provide an updated analysis of the systemic effects of long‐standing hypoxemia in children with primary congenital heart disease with a specific focus on secondary erythrocytosis and hemostasis.
Comparison of selective head cooling versus whole‐body cooling
Authors: Celik Yalcın , Atıcı Aytug , Gulası Selvi , Okuyaz Cetin , Makharoblıdze Khatuna , Sungur Mehmet Ali 
Published: July 2015

AbstractBackgroundThis study compared selective head cooling (SHC) and whole‐body cooling (WBC) in newborns with hypoxic–ischemic encephalopathy (HIE).MethodsWe conducted a prospective randomized small‐scale pilot study in newborns with HIE, born after >35 weeks of gestation. The patients were randomly assigned to receive SHC or WBC.ResultsThe SHC group consisted of 17 patients, and the WBC group, 12 patients. There was no significant difference in adverse effects related to cooling therapy between the two groups. During the 12 month study period, seven patients in the SHC group and four in the WBC group died, but the difference was not significant (P = 0.667). Among the patients alive at 12 months after treatment, six in the SHC group and four in the WBC group had severe disabilities; the difference was not significant (P = 0.671). When the composite outcome of death or severe disability was evaluated, the difference between the SHC group (77%, n = 13) and the WBC group (67%, n = 8) was not significant (P = 0.562). Moreover, the number of survivors without disability at 12 months after treatment did not differ significantly between the SHC group (n = 3) and the WBC group (n = 4; P = 0.614).ConclusionsThere were no significant differences in adverse effects, 12 month neuromotor development, or mortality rate between SHC and WBC in newborns with HIE, born after >35 weeks of gestation.
Efficacy of new leukocyte parameters versus serum C‐reactive protein, procalcitonin, and interleukin‐6 in the diagnosis of neonatal sepsis
Authors: Çelik H. Tolga , Portakal Oytun , Yiğit Şule , Hasçelik Gülşen , Korkmaz Ayşe , Yurdakök Murat 
Published: July 2015

AbstractBackgroundThe aim of this study was to investigate and compare the efficacy of the new leukocyte parameters mean neutrophil and monocyte volume (MNV, MMV), conductivity (MNC, MMC), scattering (MNS, MMS) and volume distribution width (NDW, MDW) with serum C‐reactive protein (CRP), procalcitonin (PC) and interleukin (IL)‐6 in the diagnosis of neonatal sepsis.MethodsA total of 227 newborns (132 boys, 95 girls) were analyzed. There were 116 infants in the sepsis group (proven sepsis, n = 40; clinical sepsis, n = 76) and 111 in the control group. Venous blood samples were collected from infants at the time of diagnosis and complete blood count, peripheral blood smear, blood cultures, CRP, PC, IL‐6 and MNV, MMV, MNC, MMC, MNS, MMS, NDW, and MDW were analyzed.ResultsMNV, NDW, MMV and, MDW were higher in infants with sepsis than in controls (P < 0.05 for all). MNS was lower in the patients with sepsis (P = 0.002). There was no significant difference between the sepsis and control groups in terms of MNC, MMC and MMS.ConclusionAlthough the predictive value of leukocyte parameters including neutrophil and monocyte volume, conductivity, scattering and volume distribution width in the diagnosis of neonatal sepsis was lower than that of CRP, PC and IL‐6, some of these new parameters may be useful in the differential diagnosis of newborn sepsis, along with the other screening tools. In particular, MNV seems to be the most useful parameter with the highest specificity; also, the importance of PC in the diagnosis of early onset sepsis was confirmed.
Dynamics of hydroxyoctadecadienoic acid in epilepsy patients treated with valproic acid
Authors: Azumagawa Kohji , Mino Makoto , Ishida Noriko , Shichiri Mototada , Shigeri Yasushi , Yoshida Yasukazu , Tamai Hiroshi 
Published: July 2015

AbstractBackgroundThe effects of valproic acid (VPA) on oxidative stress are controversial due to differences in experimental conditions. Recently, total hydroxyoctadecadienoic acid (tHODE), derived from linoleic acid, was proposed as a potent biomarker to evaluate oxidative stress.MethodsThe subjects consisted of 10 new‐onset epilepsy patients treated with VPA. We measured plasma tHODE consecutively for 1 year to evaluate the degree of oxidative stress and then compared plasma tHODE at the beginning and the end of experiments with the peak level. Ten age‐matched healthy subjects were recruited as a control group and their plasma tHODE was compared to the initial plasma tHODE of the epilepsy group. Measurements were done using liquid chromatography–tandem mass spectrometry.ResultsMean initial plasma tHODE in the epilepsy group was 165.2 ± 76.8 nmol/L, which was not significantly different from that of the control group (199.3 ± 62.5 nmol/L). In five epilepsy patients, plasma tHODE increased above the pathological level in 6 months, but returned to normal within 1 year. In the whole group, the difference plasma tHODE between peak, at the beginning and 1 year later, was significant.ConclusionOxidative stress generated by VPA increased temporarily, but decreased to normal after 1 year. it is reasonable to be concerned about the effects of oxidative stress, especially at the start of VPA treatment.
Detection of human cytomegalovirus (CMV) DNA in feces has limited value in predicting CMV enteritis in patients with intestinal graft‐versus‐host disease after allogeneic stem cell transplantation
Authors: Sun Y.‐Q. , Xu L.‐P. , Han T.‐T. , Zhang X.‐H. , Wang Y. , Han W. , Wang F.‐R. , Wang J.‐Z. , Chen H. , Chen Y.‐H. , Yan C.‐H. , Chen Y. , Liu K.‐Y. , Huang X.‐J. 
Published: July 2015

AbstractBackgroundCytomegalovirus (CMV) enteritis after allogeneic hematopoietic stem cell transplantation (allo‐HSCT) is difficult to diagnose. We aimed to evaluate the sensitivity and specificity of the detection of CMV DNA in feces for predicting CMV enteritis.MethodsHSCT patients with intestinal graft‐versus‐host disease (GVHD) were enrolled if they met the following criteria: (i) underwent a colonoscopy and (ii) peripheral blood and feces specimens were available for CMV DNA detection within 24 h of colonoscopy. The colonoscopy histology was used as the gold standard for diagnosing CMV enteritis.ResultsFifty‐six patients underwent 58 colonoscopy examinations, and 7 were diagnosed as having CMV enteritis. Within 24 h of colonoscopy, 9 patients had detectable CMV in the feces and 19 patients had detectable CMV in the plasma, respectively. In the 7 patients with CMV enteritis, only 2 had detectable CMV in the stool, resulting in a sensitivity of 28.6%. In the 51 patients without CMV enteritis, 44 had no detectable CMV in the stool, with a specificity of 86.3%.ConclusionWe concluded that CMV detection in the feces was not a good predictor of CMV enteritis in patients with intestinal GVHD after allo‐HSCT.
Deletion of a C–terminal intrinsically disordered region of WRINKLED1 affects its stability and enhances oil accumulation in Arabidopsis
Authors: Ma Wei , Kong Que , Grix Michael , Mantyla Jenny J. , Yang Yang , Benning Christoph , Ohlrogge John B. 
Published: July 2015

SummaryWRINKLED1 (WRI1) is a key transcription factor governing plant oil biosynthesis. We characterized three intrinsically disordered regions (IDRs) in Arabidopsis WRI1, and found that one C–terminal IDR of AtWRI1 (IDR3) affects the stability of AtWRI1. Analysis by bimolecular fluorescence complementation and yeast‐two‐hybrid assays indicated that the IDR3 domain does not determine WRI1 stability by interacting with BTB/POZ‐MATH proteins connecting AtWRI1 with CULLIN3‐based E3 ligases. Analysis of the WRI1 sequence revealed that a putative PEST motif (proteolytic signal) is located at the C–terminal region of AtWRI1IDR3. We also show that a 91 amino acid domain at the C–terminus of AtWRI1 without the PEST motif is sufficient for transactivation. We found that removal of the PEST motif or mutations in putative phosphorylation sites increased the stability of AtWRI1, and led to increased oil biosynthesis when these constructs were transiently expressed in tobacco leaves. Oil content was also increased in the seeds of stable transgenic wri1–1 plants expressing AtWRI1 with mutations in the IDR3‐PEST motif. Taken together, our data suggest that intrinsic disorder of AtWRI1IDR3 may facilitate exposure of the PEST motif to protein kinases. Thus, phosphorylation of the PEST motif in the AtWRI1IDR3 domain may affect AtWRI1‐mediated plant oil biosynthesis. The results obtained here suggest a means to increase accumulation of oils in plant tissues through WRI1 engineering.
Rice Exo70 interacts with a fungal effector, AVR‐Pii, and is required for AVR‐Pii‐triggered immunity
Authors: Fujisaki Koki , Abe Yoshiko , Ito Akiko , Saitoh Hiromasa , Yoshida Kentaro , Kanzaki Hiroyuki , Kanzaki Eiko , Utsushi Hiroe , Yamashita Tetsuro , Kamoun Sophien , Terauchi Ryohei 
Published: July 2015

SummaryVesicle trafficking including the exocytosis pathway is intimately associated with host immunity against pathogens. However, we still have insufficient knowledge about how it contributes to immunity, and how pathogen factors affect it. In this study, we explore host factors that interact with the Magnaporthe oryzae effector AVR‐Pii. Gel filtration chromatography and co‐immunoprecipitation assays identified a 150 kDa complex of proteins in the soluble fraction comprising AVR‐Pii and OsExo70‐F2 and OsExo70‐F3, two rice Exo70 proteins presumably involved in exocytosis. Simultaneous knockdown of OsExo70‐F2 and F3 totally abrogated Pii immune receptor‐dependent resistance, but had no effect on Pia‐ and Pik‐dependent resistance. Knockdown levels of OsExo70‐F3 but not OsExo70‐F2 correlated with reduction of Pii function, suggesting that OsExo70‐F3 is specifically involved in Pii‐dependent resistance. Under our current experimental conditions, over‐expression of AVR‐Pii or knockdown of OsExo70‐F2 and ‐F3 genes in rice did not affect the virulence of compatible isolates of M. oryzae. AVR‐Pii interaction with OsExo70‐F3 appears to play a crucial role in immunity triggered by Pii, suggesting a role for OsExo70 as a decoy or helper in Pii/AVR‐Pii interactions.
Complete Genome Sequence of
Citrobacter
Phage CVT22 Isolated from the Gut of the Formosan Subterranean Termite,
Coptotermes formosanus
Shiraki
Authors: Tikhe Chinmay Vijay , Martin Thomas M. , Gissendanner Chris R. , Husseneder Claudia 
Published: July 2015

ABSTRACT

The complete genome of bacteriophage CVT22 infecting
Citrobacter
sp. strain TM1552 is reported here. Both the bacteriophage and
Citrobacter
sp. TM1552 were isolated from the gut of the Formosan subterranean termite,
Coptotermes formosanus
. This is the first report of a genome sequence of a bacteriophage isolated from the termite gut.
Complete Genome Sequence of
Endomicrobium proavitum
, a Free-Living Relative of the Intracellular Symbionts of Termite Gut Flagellates (Phylum
Elusimicrobia
)
Authors: Zheng Hao , Brune Andreas 
Published: July 2015

ABSTRACT

We sequenced the complete genome of
Endomicrobium proavitum
strain Rsa215, the first isolate of the class
Endomicrobia
(phylum
Elusimicrobia
). It is the closest free-living relative of the endosymbionts of termite gut flagellates and thereby provides an excellent model for studying the evolutionary processes during the establishment of an intracellular symbiosis.
Genome Sequence of
Rhodococcus
sp. Strain RD6.2 DSM 46800, a Methanesulfonate-Degrading Strain
Authors: Henriques Ana C. , De Marco Paolo 
Published: July 2015

ABSTRACT

The complete genome sequence of a methanesulfonate-degrading strain,
Rhodococcus
sp. strain RD6.2 DSM 46800, which was isolated from a brackish marsh sediment sample, is described here. This is the first reported genome of a nonproteobacterial strain using methanesulfonate (MSA) as a sole source of carbon and energy, which does not possess the conventional MSA-monooxygenase (MSAMO).
Complete Genome Sequence of “
Candidatus
Liberibacter africanus,” a Bacterium Associated with Citrus Huanglongbing
Authors: Lin Hong , Pietersen Gerhard , Han Cliff , Read David Alan , Lou Binghai , Gupta Goutam , Civerolo Edwin L. 
Published: July 2015

ABSTRACT

We report here the complete genome sequence of “
Candidatus
Liberibacter africanus” strain PTSAPSY. The 1,192,232-bp genome with 34.5% G+C content comprises 1,017 open reading frames, 44 tRNAs, and three complete rRNAs in a circular chromosome.
Draft Genome Sequence of a Taxonomically Unique
Neisseria
Strain Isolated from a Greater White-Fronted Goose (
Anser albifrons
) Egg on the North Slope of Alaska
Authors: Hansen Cristina M. , Choi Sang Chul , Parker Jayme , Hueffer Karsten , Chen Jack 
Published: July 2015

ABSTRACT

We report here the draft genome sequence of a unique
Neisseria
strain that was isolated from a greater white-fronted goose (
Anser albifrons
) egg. The sequencing was performed with an Illumina MiSeq system, and the sequence consists of 275 contigs. The total genome is 2,397,978 bp long and has a G+C content of 46.4%.
Genome Sequence of Paenibacillus wulumuqiensis sp. nov., a Bioflocculant-Producing Species
Authors: Wu Qian , Zhu Liying , Jiang Ling , Xu Xian , Xu Qing , Zhang Zhidong , Huang He 
Published: July 2015

ABSTRACT

Paenibacillus wulumuqiensis
sp. nov. is a novel strain that can produce bioflocculants. Here, we report 5.37-Mb assembly of its genome sequence and other useful information, including the coding sequences (CDSs) responsible for the biosynthesis of polysaccharide-based bioflocculants, cold-shock protein, and vitamin production.
Complete Genome Sequence of Noninvasive Streptococcus pyogenes M/
emm28
Strain STAB10015, Isolated from a Child with Perianal Dermatitis in French Brittany
Authors: de Andrade Barboza Suzane , Meygret Alexandra , Vincent Pascal , Moullec Séverine , Soriano Nicolas , Lagente Vincent , Minet Jacques , Kayal Samer , Faili Ahmad 
Published: July 2015

ABSTRACT

We report here the complete genome sequence of a noninvasive strain of
Streptococcus pyogenes
M/
emm28
, isolated from perianal dermatitis in a child. The genome is composed of 1,950,454 bp, with a G+C content of 38.2%, and it has 1,925 identified coding sequences and harbors two intact prophages and a new integrating conjugative element (ICE).
Draft Genome Sequence of Pseudomonas frederiksbergensis SI8, a Psychrotrophic Aromatic-Degrading Bacterium
Authors: Ruiz Oscar N. , Brown Lisa M. , Striebich Richard C. , Mueller Susan S. , Gunasekera Thusitha S. 
Published: July 2015

ABSTRACT

Pseudomonas frederiksbergensis
strain SI8 is a psychrotrophic bacterium capable of efficient aerobic degradation of aromatic hydrocarbons. The draft genome of
P. frederiksbergensis
SI8 is 6.57 Mb in size, with 5,904 coding sequences and 60.5% G+C content. The isopropylbenzene (cumene) degradation pathway is predicted to be present in
P. frederiksbergensis
SI8.
Assessing the effect of Michigan's smoke-free law on air quality inside restaurants and casinos: a before-and-after observational study
Authors: Shamo Farid , Wilson Teri , Kiley Janet , Repace James 
Published: July 2015

ObjectivesTo assess the effect of Michigan's smoke-free air (SFA) law on the air quality inside selected restaurants and casinos. The hypothesis of the study: if the SFA law is effectively implemented in restaurants and casinos, there will be a significant reduction in the particulate matter PM2.5measured in the same establishments after the law is implemented.SettingPrelaw and postlaw design study.Participants78 restaurants in 14 Michigan cities from six major regions of the state, and three Detroit casinos.MethodsWe monitored the real-time PM2.5in 78 restaurants and three Detroit casinos before the SFA law, and again monitored the same restaurants and casinos after implementation of the law, which was enacted on 1 May 2010.Primary and secondary outcome measuresConcentration measurements of secondhand smoke (SHS) fine particles (PM2.5) were compared in each restaurant in the prelaw period to measurements of PM2.5in the same restaurants during the postlaw period. A second comparison was made for PM2.5levels in three Detroit casinos prelaw and postlaw; these casinos were exempted from the SFA law.ResultsPrelaw data indicated that 85% of the restaurants had poor to hazardous air quality, with the average venue having ‘unhealthy’ air according to Michigan's Air Quality Index for PM2.5. Postlaw, air quality in 93% of the restaurants improved to ‘good’. The differences were statistically significant (p<0.0001). By comparison, the three casinos measured had ‘unhealthy’ air both before and after the law.ConclusionsThe significant air quality improvement in the Michigan restaurants after implementation of the SFA law indicates that the law was very effective in reducing exposure to SHS. Since the Detroit casinos were exempted from the law, the air quality was unchanged, and remained unhealthy in both prelaw and postlaw periods.