bluuebunny/crossref_metadata_2025_split huggingface dataset:
Urticarial Vasculitis with Circulating Immune Complexes and Mixed Cryoglobulins: Studies of Pathogenesis
Authors: Yasue Takashi , Yasue Atsuko 
Published: April 2015

AbstractA 64‐year‐old woman with urticarial vasculitis associated with mixed IgM‐IgG‐C cryoglobulinemia and circulating immune complexes developed skin lesions, which were urticaria‐like during the stages when cryoglobulinemia and hypocomplementemia were found but mimicked erythema multiforme and anaphylactoid purpura when there were no such findings. Typical leukocytoclastic vasculitis was observed in lesions of the latter type.Granular deposits of immunoglobulins (IgG, IgM) and complement components (Clq, C3) were found in the dermal‐epidermal junction, in the dermal blood vessel walls, and in the dermal connective tissue of the involved and uninvolved skin. IgM deposits predominated during the cryoglobulin‐positive and IgG deposits during the cryoglobulin‐negative stage of the disease. Perivascular neutrophilic infiltration and edema were observable in the uninvolved skin adjacent to the lesion. Electron microscopy revealed microtubular structures in the vascular endothelium of the involved skin.Systemic low or moderate doses of corticosteroids (prednisolone, <20 mg/day) were not very effective but dapsone or colchicine elicited an excellent response.Apparently, Clq precipitin, mixed IgM‐IgG cryoglobulins and IgG‐rheumatoid factor complexes are all capable of inducing urticarial vasculitis; these substances have biological activities similar to those of immune complexes.
Sebaceous Nevus with Sweat‐gland Duct Proliferation around the Suture Material
Authors: Nishitani Keiko , Kiryu Hiromaro 
Published: April 2015

AbstractIn a Japanese girl with sebaceous nevus, ductal structure proliferation appeared around the suture material.An immunohistochemical study revealed that the ductal structure was of sweat‐gland origin. There has been no recurrence after 5 years.
Comparison of Therapeutic Efficacy of Topical PUVA, Oral Etretinate, and Combined PUVA and Etretinate for the Treatment of Psoriasis and Development of PUVA Lentigines and Antinuclear Antibodies
Authors: Takashima Akira , Sunohara Akiyo , Matsunami Eriko , Mizuno Nobuyuki 
Published: April 2015

AbstractSixty‐two and 38 psoriatic patients were treated with topical PUVA and combined etretinate and topical PUVA (Re‐PUVA), respectively. In both groups, 50% of the patients showed initial recovery after 6 weeks and over 90% after 14 weeks. Re‐PUVA was more effective than PUVA alone in obtaining complete clearance (p<0.05). To clear psoriasis in 50% of the patients, PUVA and Re‐PUVA required 63 and 26 weeks, respectively. Furthermore, the integrated clearance rates after 70 weeks were 50% in PUVA and 63% in Re‐PUVA. Each therapy showed a similar remission period; psoriasis recurred in 50% of the patients after 4 months. In addition, 17 patients were treated with oral etretinate, and Re‐PUVA was found to be more effective than etretinate monotherapy. Another aim was to determine whether etretinate would inhibit the development of PUVA side effects. Adding etretinate failed to inhibit the production of PUVA lentigines but clearly suppressed antinuclear antibody (ANA) expression. Six of 56 patients treated with PUVA alone developed ANA during the treatment. In marked contrast (p=0.05), ANA was detected in none of 34 patients treated with Re‐PUVA.
Erythrocyte Glutathione Peroxidase Activity and Plasma Vitamin E Status in Patients with Psoriasis
Authors: Jain Vijay K. , Bansal Ramesh K. , Aggarwal S.K. , Chaudhary S.D. , Saini A.S. 
Published: April 2015

AbstractErythrocyte selenium dependent glutathione peroxidase (GSH‐Px) activity and plasma vitamin E levels were measured in 20 psoriatics. Psoriatics had significantly lower GSH‐Px and plasma vitamin E levels than did controls. Psoriatics were divided into three grades by the rule of nine: Grade I—less than 33% skin involvement, Grade II—33‐66%, and Grade III—over 66% skin involvement. Grade III psoriatics showed much lower values of GSH‐Px and plasma vitamin E, showing a definite correlation with the percentage of surface area involved.
The Intra‐cellular Location of Disulfide‐bond‐rich Particles
Authors: Tezuka Tadashi , Takahashi Masae , Miyamoto Hiroyasu 
Published: April 2015

AbstractFour micron cryostat sections of three‐day‐old rat skin were fixed in 98% ethanol at −20°C for 4 minutes. These sections were preincubated in 10mM phosphate buffered saline, pH 7.4, containing 10mM 2‐mercaptoethanol at 37°C for 30 minutes and washed thoroughly in the same buffer. After all the free sulfhydryl groups were blocked with N‐ethylmaleimide and disulfide bonds were again reduced with 2‐mercaptoethanol, the section was reacted with 7‐(N‐dimethylamino‐4‐methyl‐3‐coumarinyl) maleimide. Many minute fluorescent particles were seen in the stratum granulosum of the epidermis, especially of the acrotrichium. Cryostat sections containing an acrotrichium were fixed again in 2.5% glutaraldehyde and embedded in Epon. Thin sections were examined under the electron microscope. The location of the disulfide bond‐rich particles around an acrotrichium was examined and compared with the location of these fluorescent particles. These disulfide‐bond‐rich particles were found to be low electron dense particles 0.3 to 0.7 μm in diameter located in or around the keratohyalin granules.
Giant Nevus Lipomatosus Superficialis Associated with Cavernous Hemangioma
Authors: Hann Seung Kyung , Yang Dong Soon , Lee Seung Hun 
Published: April 2015

AbstractWe report a case of NLS that was unusual. Many nodules coalesced to form a giant cerebriform mass and a large plaque confined to the back where comedo‐like plugs were present on the surface. Histopathologic examination of the cerebriform mass showed mature ectopic adipose tissue and cavernous hemangioma in the dermis.
Aminoisobutyric Acid Uptake in Normal and Transformed Human Epidermal Keratinocytes
Authors: Verrando Patrick , Ortonne Jean‐Paul 
Published: April 2015

Abstractα‐Aminoisobutyric acid transport has been demonstrated in cultured human epidermal keratinocytes as well as in the transformed state of these cells. The concentrative uptake is sodium‐dependent and may be ascribed to one Michaelis‐Menten component whose maximal velocity is 6.9 nmol/min × 106 cells, with an apparent affinity of 3.8 mM. These parameters may be modified, depending upon the nature of malignant transformation. In SVK14 SV40‐virus‐transformed cells, there is no change of affinity but the maximal velocity is 1.5 fold less than in normal cells. In the spontaneous epidermoid carcinoma‐derived A431 line, this phenomenon is inverted; the maximal velocity is unmodified but the system affinity is 2.2‐fold higher than in normal cells. The unresponsiveness of this transport system to insulin and epidermal growth factor (EGF) shows that it behaves differently from those of many other cell types.
Comparison of Serum Gastrin Levels during Topical and Systemic Administration of Corticosteroids
Authors: Tanaka Keiko , Itoh Tadao , Shimao Shuhei 
Published: April 2015

AbstractSerum gastrin levels did not change during topical corticosteroid therapy with an experimental dose of 20 to 60 g/day for seven consecutive days, and serum 11‐hydroxycorticosteroid levels were significantly suppressed during the treatment. On the other hand, after daily oral administration of 1.5 mg/day betamethasone for seven consecutive days in volunteers who had fasted overnight, serum gastrin levels were higher and 11‐hydroxycorticosteroid levels were significantly lower.Fasting serum gastrin levels were significantly increased in rats after intraperitoneal administration of prednisolone at dosages of 2, 10, 20, or 50 mg/kg/day, but they were not significantly altered after the administration of prednisolone at 0.5 mg/kg/day. Serum gastrin levels were not significantly altered in man or rats after the administration of a histamine H1 receptor antagonist. Therefore, serum gastrin levels are apparently not influenced by topical corticosteroid treatment although they are altered after systemic corticosteroid administration.
Combination of Photochemotherapy (PUVA) and Ultraviolet B (UVB) in the Treatment of Psoriasis Vulgaris
Authors: Park Yoon‐Kee , Kim Hyung Joo , Koh Young Jin 
Published: April 2015

AbstractNineteen patients with psoriasis vulgaris were treated with a combination of psoralen‐ultraviolet A (PUVA) and ultraviolet B (UVB) on the right side of their bodies and with PUVA therapy alone on the left side. Herein is an analysis of the results. There were no significant differences in the mean number of treatments, the mean UVA dose at clearing, or the mean cumulative UVA dose between the PUVA‐UVB side and the PUVA side. However, in 4 cases, the PUVA‐UVB side cleared more rapidly than the PUVA side. Interestingly, patients who received PUVA‐UVB on one side and PUVA on the other required fewer treatments, a lower ultraviolet (UV) dose at clearing, and a lower cumulative UV dose than did patients who were treated with only PUVA monotherapy or UVB monotherapy, following the same protocol.This combined method may be useful in the treatment of chronic psoriatic patients, because of rapid clearing and a marked reduction in the total cumulative UV radiation. However, further follow‐up studies are indicated due to the long‐term side effects of combined UV radiation.
Follicular Mucinosis: Response to Indomethacin
Authors: Kodama Hajime , Umemura Shigeo , Nohara Nozomi 
Published: April 2015

AbstractA patient with follicular mucinosis was treated topically and orally with indomethacin. He had generalized plaques and grouped follicular papules but no signs of cutaneous lymphoma. The skin lesions responded favorably to topical application. Oral administration produced a dramatic improvement. No recurrence has been seen for 5 years after stopping the treatment.
Eosinophil Chemotactic Activity of Collagen‐derived Fragments
Authors: Isoda Midori 
Published: April 2015

AbstractEosinophil and neutrophil chemotactic activities of some collagens and their collagenase‐digested fragments were measured by using a Boyden chamber method. Fragments released from bovine dermal collagen and type III collagen derived from calf skin showed chemotactic activity for guinea pig eosinophils in a dose‐dependent manner, but collagen itself did not. Fragments released from bovine dermal collagen also showed chemotactic activity for human neutrophils. Gel filtration study indicated that some sizes of this collagenase‐digested fragments had chemotactic activities for eosinophils.These results suggest that collagen‐derived fragments may play a role in tissue eosinophilia in some inflammatory lesions.
Trace Element Analyses in Pseudoxanthoma Elasticum
Authors: Kanzaki Tamotsu , Matsuno Takashi , Kamimura Kimio , Miyazawa Shichiro , Nishiyama Shigeo 
Published: April 2015

AbstractThe dermis and sera of 11 patients with pseudoxanthoma elasticum (PXE) were analyzed by several techniques which test for the presence and quantity of trace elements (Fe, Ni, Co, Cu, Mn, Cr, Zn, Pb, Cd, Al, S, K, Cl, Ca and P). The investigative methods included 1) electron microscopic point X‐ray microanalysis, 2) inductively coupled plasma atomic emission spectrophotometric analysis (ICP‐AES), 3) X‐ray diffraction analysis and/or 4) atomic absorption spectro‐photometric analysis. 1) Among 7 specimens of dermis showing histological degeneration of the elastic fibers, 2 showed prominent levels of A1. In early degeneration of the elastic fibers, Ca was present in only small amounts; however, in more fully developed lesions, large quantities of both Ca and P were found, while Fe, Co, Ma and Zn were detected in trace amounts. 2) ICP‐AES analysis confirmed large deposits of Al, Ca, and P in diseased dermis (Al: 32.1 ppm wet tissue); other elements were found in normal quantities. Al was not detected in the control dermis. 3) X‐ray diffraction analysis suggested the pattern of hydroxyapatite [Ca10(PO4)6(OH)2], which indicates that most of the Ca exists in a crystalline form. 4) Atomic absorption spectrophotometric analysis confirmed a large concentration of Al (11–21 μg/dl, mean=17.2; normal <10) in all test patient's sera. The combined analysis revealed an increased amount of Al in patient's sera as well as in the elastic fibers of the dermis at an early stage of this disease (PXE). These findings suggest a possible role for Al in the pathogenesis of PXE.
Malignant Melanoma in Malaysia
Authors: Ismail Rokiah , Meng Looi Lai 
Published: April 2015

AbstractThis paper reviews the malignant melanomas seen at the University Hospital, Kuala Lumpur, Malaysia, over a period of twelve years. During this period, there were twenty‐three cases of histologically proven malignant melanoma. Data from the case records was studied and analysed. Nineteen cases had primary cutaneous malignant melanoma; of these, eight had a history of recent onset, while the rest had pre‐existing moles.The largest number of cases (14) was seen at the surgical unit. In terms of ethnic distribution, the Chinese constituted the largest group (16 cases); three were Indians, one Malay, one Orang Asli (Malay Aborigine) and two Caucasians. All age groups above eighteen years were affected and there was a male to female ratio of 1.3:1.Biopsy specimens were blocked in paraffin. The stains used were hematoxylin and eosin and Masson‐Fontana. Of the cutaneous melanomas, nodular melanoma had the highest incidence of metastases at the time of biopsy. One notable feature was the poor lymphoplasmacytic response to these tumors. Prognosis was worse in nodular melanomas with an invasive level of 3 and beyond and tumor thickness of more than 4 mm.
A Keratotic Variant of Malignant Trichilemmoma
Authors: Tokura Yoshiki , Koide Masayo , Takigawa Masahiro , Yamada Mizuho 
Published: April 2015

AbstractAtypical changes were found in the lesional epidermal cells of a case of follicular tumor with massive horny material. Our case seemed to represent a keratotic variant of malignant trichilemmoma.
Sarcoid Reactions in a Patient with Congenital Syphilis
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Imai Toshiya , Ito Fumiyuki , Nishihara Kiyoshi 
Published: April 2015

AbstractA case of sarcoid reactions in a patient with congenital syphilis was reported. The eruptions appeared on the upper extremities and disappeared within 10 days after biopsy. Histological findings were compatible with those of subcutaneous sarcoidosis. The patient also had so‐called Hutchinson's teeth, rhagades of the lips, corneal scars and eighth nerve deafness.
Genital Paget's Disease: A Transmission‐, Scanning‐, and Immuno‐electron Microscopic Study
Authors: Miyamoto Toru , Mihara Motoyuki , Ishihara Masahiko , Hagari Yoshitaka , Shimao Shuhei 
Published: April 2015

AbstractA case of genital Paget's disease examined by a transmission‐, scanning‐, and immuno‐electron microscopy was reported. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) observations indicated that Paget cells had the characteristic features of glandular cells. In immunoelectron microscopy, carcinoembryonic antigen was found on the entire plasma membrane of Paget cells. Therefore, these observations may support the viewpoint that Paget cells are neoplasmic glandular cells.
Experimental Chronic Dermatophytosis in Human Skin Grafted to Nude Mouse: Inoculation of Trichophytons and Histopathological Evaluation
Authors: Kakutani Hiroyuki , Takahashi Shin‐ya 
Published: April 2015

AbstractTwo strains of T. rubrum and one strain of T. mentagrophytes were inoculated into human skin grafted onto BALB/c nude mice by the needle puncture method. Infection was established in 1 of the 10 animals inoculated with fluffy colony type T. rubrum, 2 of the 10 animals inoculated with powdery colony type T. rubrum, and 7 of the 10 animals inoculated with granular colony type T. mentagrophytes, suggesting that the skin grafts are infectible by anthropophilic and zoophilic strains of dermatophytes. T. rubrum infection continued for a maximum of 9 weeks and T. mentagrophytes infection for more than 11 weeks. In the animals inoculated with T. mentagrophytes, fungal elements were localized in the stratum corneum of the human skin grafts. In the acute stage, microabscesses consisting of neutrophils were observed under the stratum corneum in contact with fungal elements; in the chronic stage, epidermal thickening and infiltration, mainly consisting of histiocytes and a smaller number of lymphocytes, was noted in the upper and middle dermis. Ultrastructural findings from the parasites were similar to those of dermatophytosis in man. This experimental system should be useful as a model of chronic dermatophyte infection in the human skin.
Monocyte‐modulating Activities in the Sera of Patients with Granuloma Annulare
Authors: Baba Toru , Yamaguchi Koji , Hoshino Minoru , Uyeno Kenichi 
Published: April 2015

AbstractStudies were performed to determine whether the sera of the patients with granuloma annulare had monocyte modulating activities. As indicators of monocyte modulation, spreading and aggregation were used. As a control, similar experiments were performed on pooled normal human sera. Normal human monocytes were incubated with either test sera or control sera. The percentage of spread cells was estimated at 6 hr of incubation and that of aggregated cells was estimated at 24 hr of incubation. The percentage of spread cells and aggregated cells in the monocytes incubated with granuloma annulare sera was higher than that of the cells incubated with pooled normal human sera. Thus, granuloma annulare sera may have a greater ability to induce monocytes to spread and aggregate than do normal human sera.
Whole Mount Technique: An Improved Hamster Ear Model to Evaluate Pharmacologic Effects on Sebaceous Glands
Authors: Motoyoshi Katsuhiro 
Published: April 2015

AbstractAn improved and simplified hamster ear model (whole mount technique; WMT) is described. Excised ear skin specimens were incubated in 2N NaBr at 37°C for 2 hours, yielding a sheet of dermis with the sebaceous glands attached. After Sudan III staining, the areas of the sebaceous glands in the sheet were measured with an image analyzing system. The ratios of a mean area of androgen‐stimulated sebaceous glands/a mean area of non‐treated sebaceous glands (AS/NT) were 2.56 in females and 1.69 in males; these results closely resemble AS/NT ratios found in histological sagittal sections. Over 1.0% concentrations of topically applied cyproterone acetate were highly effective in suppressing the proliferation of sebaceous glands in androgen‐stimulated hamsters, as measured by WMT. Since no routinely processed histological sections are necessary and the exact size of a whole sebaceous gland can be obtained by WMT, this method seems to be useful for routine work to assess the efficacy of topically applied anti‐comedogenic or anti‐lipogenic drugs.
Study of the Skin of a New Hairless Rat Mutant
Authors: Hanada Katsumi , Chiyoya Shigehito , Suzuki Kenji , Hashimoto Isao , Hatayama Ichiro 
Published: April 2015

AbstractHistological and immunological examinations were performed on the skin of a newly established strain of hairless rat (Hirosaki hairless rat; HHR). The appearance of the mutant rat skin was marked by resembles to human skin with wrinkles and scarce vellus hair. The presence of scattered dilated follicular cysts in the mid‐dermis was the most predominant microscopic change. Electron microscopically, some of the basal cells contained many oval organelles in the cytoplasm, although their role was not clear. Langerhans cells were observed, but melanocytes were lacking in the epidermis. Immunofluorescence technique demonstrated Ia‐positive cells (presumably Langerhans cells) in epidermal sheets from the soles of the mutants, which also developed contact sensitivity to dinitrochlorobenzene (DNCB). HHRs may be a useful experimental model for studies of allergic as well as nonallergic dermatitis requiring gross observation and wrinkled skin.
Echographic Studies of Superficial Lymphadenopathies
Authors: Miyauchi Shunji , Murakami Shinji , Miki Yoshiharu 
Published: April 2015

AbstractSuperficial lymphadenopathies were examined by sector B‐mode echography using a 5 or 10 MHz transducer and compared with microscopic sections. A total of 54 lymph nodes in the inguinal, cervical or axillary regions was studied in 54 patients; the primary skin lesions were benign in 35 and malignant in 19 of these patients.Histologically verified and non‐verified, reactive lymphadenopathies showed echolucent nodes with central or medullary echoes; the attenuation of the sensitivity was under 10 decibels (dB). Histologically, the medullary echo corresponded to the medulla of the lymph node and the echolucent zone to the cortex. Tuberculous lymphadenitis, hyalinized, severe fibrosis from foreign body granuloma, and squamous cell carcinoma nests showed irregular, cortical echoes at 0 to 5 dB. Total replacement of the lymph node by the metastases of malignant melanoma or malignant lymphoma showed echolucent nodes without internal echoes, even at 0 dB.
Multiple Mixed Tumor (Pleomorphic Adenoma): A Clinicopathological Study
Authors: Nakamura Shin‐ichi , Nakayama Keiji , Ito Fumiyuki , Furuya Kiyohisa 
Published: April 2015

AbstractA case of a 72‐year‐old man with multiple mixed tumor (pleomorphic adenoma) with its clinicopathological study was reported. In this patient, the first tumor appeared in the right parotid gland area of his right maxillary region at the age of 20. After resection of the primary tumor, the tumor recurred at the same site; he was operated on 2 more times at the ages of 21 and 45. Additionally, other new tumors appeared on the skin of the right ear lobe at the age of 48 and on the right temporal region at the age of 51. The most recent tumor reappeared in his right parotid gland area at the age of 60.All the tumors were examined by light microscope and electron microscope and the diagnosis of multiple mixed tumor (pleomorphic adenoma) was established.
Acid Cholesterol Esterase Activity in the Foam Cells Isolated from Rabbit Experimental Xanthoma Tissues
Authors: Kodama Hajime , Nagao Yo , Arakawa Kenzo , Akiyama Hisanori , Akagi Osamu , Nohara Nozomi 
Published: April 2015

AbstractAcid cholesterol esterase (ACE) activity was assayed in the 10,000×g pellet from infiltrating cells isolated from experimental rabbit xanthoma tissues. Skin specimens were obtained from sites of intradermal dextran sulfate injections on a normolipemic rabbit (NLR) and a diet‐induced hypercholesterolemic rabbit (HCR). Most infiltrating cells were histiocytes or foam cells. Histiocytes from the NLR did not accumulate cholesteryl esters after the injections. Accumulation of cholesteryl esters in the xanthoma tissues of the HCR increased after repeating the injections at the same site. The ACE activity was greater in the cholesteryl ester‐accumulated foam cells than in histiocytes. The enzyme activity in the foam cells decreased during the developmental course of cholesteryl ester accumulation. These findings indicate that lysosomal ACE is activated and hydrolyzes the internalized cholesteryl esters of serum lipoprotein origin in the histiocytes which are transforming into foam cells. However, the enzyme activity decreases when too much of the cholesteryl esters has accumulated in the foam cells.