bluuebunny/crossref_metadata_2025_split huggingface dataset:
Self-rated and observer-rated measures of well-being and distress in adolescence: an exploratory study
Authors: Vescovelli Francesca , Albieri Elisa , Ruini Chiara 
Published: September 2014

AbstractThe evaluation of eudaimonic well-being in adolescence is hampered by the lack of specific assessment tools. Moreover, with younger populations, the assessment of positive functioning may be biased by self-report data only, and may be more accurate by adding significant adults’ evaluations. The objective of this research was to measure adolescents’ well-being and prosocial behaviours using self-rated and observer-rated instruments, and their pattern of associations. The sample included 150 Italian high school adolescents. Observed-evaluation was performed by their school teachers using the Strengths and Difficulties Questionnaire. Adolescents completed Ryff’s Psychological Well-being Scales and Symptom Questionnaire. Pearson’ r correlations and Linear regression were performed. Self-rated dimensions of psychological well-being significantly correlated with all observer-rated dimensions, but Strengths and Difficulties Emotional symptom scale. Multiple linear regression showed that the self-rated dimensions Environmental Mastery and Personal Growth, and surprisingly not Positive Relations, are related to the observer-rated dimension Prosocial Behaviour. Adolescents with higher levels of well-being in specific dimensions tend to be perceived as less problematic by their teachers. However, some dimensions of positive functioning present discrepancies between self and observer-rated instruments. Thus, the conjunct use of self-reports and observer-rated tools for a more comprehensive assessment of students’ eudaimonic well-being is recommended.
The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastoma
Authors: van Roosmalen Ingrid A M , Reis Carlos R , Setroikromo Rita , Yuvaraj Saravanan , Joseph Justin V , Tepper Pieter G , Kruyt Frank A E , Quax Wim J 
Published: September 2014

Abstract
Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumour in humans and is highly resistant to current treatment modalities. We have explored the combined treatment of the endoplasmic reticulum (ER) stress-inducing agent 2,5-dimethyl-celecoxib (DMC) and TNF-related apoptosis-inducing ligand (TRAIL WT) or the DR5-specific TRAIL D269H/E195R variant as a potential new strategy to eradicate GBM cells using TRAIL-resistant and -sensitive GBM cells. GBM cell lines were investigated for their sensitivity to TRAIL, DMC and combination of both agents. Cell viability was measured by MTS assay and apoptosis was assessed by Annexin V/PI and acridine orange staining. Caspase activation and protein expression levels were analysed with Western blotting. Death Receptor (DR) cell surface expression levels were quantified by flow cytometry. DR5 expression was increased in U87 cells by ectopic expression using a retroviral plasmid and survivin expression was silenced using specific siRNAs. We demonstrate that A172 expresses mainly DR5 on the cell surface and that these cells show increased sensitivity for the DR5-specific rhTRAIL D269H/E195R variant. In contrast, U87 cells show low DR cell surface levels and is insensitive via both DR4 and DR5. We determined that DMC treatment displays a dose-dependent reduction in cell viability against a number of GBM cells, associated with ER stress induction, as shown by the up-regulation of glucose-regulated protein 78 (GRP78) and CCAAT/-enhancer-binding protein homologous protein (CHOP) in A172 and U87 cells. The dramatic decrease in cell viability is not accompanied by a correspondent increase in Annexin V/PI or caspase activation typically seen in apoptotic or/and necrotic cells within 24h of treatment. Although DMC did not affect DR5 expression in the GBM cells, it increased TRAIL-induced caspase-8 activation in both TRAIL-sensitive and -resistant cells, indicating that DMC potentiates initiator caspase activation in these cells. In A172 cells, sub-toxic concentrations of DMC greatly potentiated TRAIL-induced apoptosis. Furthermore, DMC strongly reduced survivin expression in A172 and U87 cells and silencing of this anti-apoptotic protein partially sensitized cells to TRAIL-induced apoptosis. Our findings corroborate that DMC is a promising agent against GBM, and uncovers a potential synergistic cooperation with TRAIL in this highly malignant cancer.
OsSUV3 transgenic rice maintains higher endogenous levels of plant hormones that mitigates adverse effects of salinity and sustains crop productivity
Authors: Sahoo Ranjan Kumar , Ansari Mohammad Wahid , Tuteja Renu , Tuteja Narendra 
Published: September 2014

Abstract

Background
The SUV3 (suppressor of Var 3) gene encodes a DNA and RNA helicase, which is localized in the mitochondria. Plant SUV3 has not yet been characterized in detail. However, the Arabidopsis ortholog of SUV3 (AT4G14790) has been shown to be involved in embryo sac development. Previously, we have reported that rice SUV3 functions as DNA and RNA helicase and provides salinity stress tolerance by maintaining photosynthesis and antioxidant machinery. Here, we report further analysis of the transgenic OsSUV3 rice plants under salt stress.

Findings
The transgenic OsSUV3 overexpressing rice T1 lines showed significantly higher endogenous content of plant hormones viz., gibberellic acid (GA3), zeatin (Z) and indole-3-acetic acid (IAA) in leaf, stem and root as compared to wild-type (WT), vector control (VC) and antisense (AS) plants under salt (200 mM NaCl) stress condition. A similar trend of endogenous plant hormones profile was also reflected in the T2 generation of OsSUV3 transgenic rice under defined parameters and stress condition.

Conclusions
In response to stress, OsSUV3 rice plants maintained plant hormone levels that regulate the expression of several stress-induced genes and reduce adverse effects of salt on plant growth and development and therefore sustains crop productivity.
VISPA: a computational pipeline for the identification and analysis of genomic vector integration sites
Authors: Calabria Andrea , Leo Simone , Benedicenti Fabrizio , Cesana Daniela , Spinozzi Giulio , Orsini Massimilano , Merella Stefania , Stupka Elia , Zanetti Gianluigi , Montini Eugenio 
Published: September 2014

AbstractThe analysis of the genomic distribution of viral vector genomic integration sites is a key step in hematopoietic stem cell-based gene therapy applications, allowing to assess both the safety and the efficacy of the treatment and to study the basic aspects of hematopoiesis and stem cell biology. Identifying vector integration sites requires ad-hoc bioinformatics tools with stringent requirements in terms of computational efficiency, flexibility, and usability. We developed VISPA (Vector Integration Site Parallel Analysis), a pipeline for automated integration site identification and annotation based on a distributed environment with a simple Galaxy web interface. VISPA was successfully used for the bioinformatics analysis of the follow-up of two lentiviral vector-based hematopoietic stem-cell gene therapy clinical trials. Our pipeline provides a reliable and efficient tool to assess the safety and efficacy of integrating vectors in clinical settings.
Considerations for animal models of blast-related traumatic brain injury and chronic traumatic encephalopathy
Authors: Goldstein Lee E , McKee Ann C , Stanton Patric K 
Published: September 2014

AbstractThe association of military blast exposure and brain injury was first appreciated in World War I as commotio cerebri, and later as shell shock. Similar injuries sustained in modern military conflicts are now classified as mild traumatic brain injury (TBI). Recent research has yielded new insights into the mechanisms by which blast exposure leads to acute brain injury and chronic sequelae, including postconcussive syndrome, post-traumatic stress disorder, post-traumatic headache, and chronic traumatic encephalopathy, a tau protein neurodegenerative disease. Impediments to delivery of effective medical care for individuals affected by blast-related TBI include: poor insight into the heterogeneity of neurological insults induced by blast exposure; limited understanding of the mechanisms by which blast exposure injures the brain and triggers sequelae; failure to appreciate interactive injuries that affect frontal lobe function, pituitary regulation, and neurovegetative homeostasis; unknown influence of genetic risk factors, prior trauma, and comorbidities; absence of validated diagnostic criteria and clinical nosology that differentiate clinical endophenotypes; and lack of empirical evidence to guide medical management and therapeutic intervention. While clinicopathological analysis can provide evidence of correlative association, experimental use of animal models remains the primary tool for establishing causal mechanisms of disease. However, the TBI field is confronted by a welter of animal models with varying clinical relevance, thereby impeding scientific coherence and hindering translational progress. Animal models of blast TBI will be far more translationally useful if experimental emphasis focuses on accurate reproduction of clinically relevant endpoints (output) rather than scaled replication of idealized blast shockwaves (input). The utility of an animal model is dependent on the degree to which the model recapitulates pathophysiological mechanisms, neuropathological features, and neurological sequelae observed in the corresponding human disorder. Understanding the purpose of an animal model and the criteria by which experimental results derived from the model are validated are critical components for useful animal modeling. Animal models that reliably demonstrate clinically relevant endpoints will expedite development of new treatments, diagnostics, preventive measures, and rehabilitative strategies for individuals affected by blast TBI and its aftermath.
Tibia bone properties at different time course of ovariectomized rats
Authors: Noor Zairin , Kania Nia , Setiawan Bambang 
Published: September 2014

Abstract
Background
The model of bilaterally ovariectomized rats mimics the accelerated bone loss observed in postmenopausal women due to estrogen deficiency. Although calcium is main mineral in bone, previous study in human showed there is hypermineralization and higher calcium level in hydroxyapatite crystal structure from osteoporosis patients. This study was aimed to investigate the effect of time course ovariectomized on tibia bone turn over markers, mineral elements, hydroxyapatite crystale, mesostructure, and histomorphometry.

Methods
A total of 30 Wistar female rats were randomly assigned into three groups (n = 10 each): control group, ovariectomy group follow up for one month and two month. All animals procedures was according to Animal Ethics Guidelines and approval by ethic committee of the Medical Faculty, Lambung Mangkurat University which obtained prior the study. Expression of osteocalcin (OC) and C-telopeptyde collagen type I (CTX) was analyzed by ELISA method. Tibia bone mineral element was measured using X-Ray Fluorescence. Hydroxyapatite crystale structure was analyzed using X-Ray Diffracttion. Mesostructure was determined using Scanning Electron Microscope. Histomorphometry was analyzed using BoneJ software analyzer. ANOVA test was used to analyze the different level of serum bone turnover markers and bone mineral elements.

Results
Serum OC and CTX were significantly decrease in one month and two month after ovariectomized groups compared to sham-operated group (P < 0.05). The levels Ca, P, Fe, Cu, Zn, Ni, Ca/P, and Cu/Zn were not significantly different in all groups (P > 0.05). The structure of hydroxyapatite crystal in one month and two month after ovariectomized groups were different compared with sham-operated control group. Mesostructure of tibia bone after one and two month ovariectomized procedure significantly different than that in sham-operated rats. The level of trabecular volume were lower significantly on OVX-1 and OVX-2 groups compared with sham group (P < 0.05). The trabecular thickness and spacing were increase significantly on OVX-1 and OVX-2 groups compared with sham group (P < 0.05). The trabecular number were significantly decrease OVX-1 and OVX-2 groups than that sham group (P < 0.05).

Conclusion
We found that two month after ovariectomized decrease serum osteocalcin but not change bone mineral elements in rats. Also, we found the difference of lattice parameter of hydroxyapatite crystale structure and trabecular properties which determined bone mesostructure.
Medication management following first-episode psychosis in young people: a developmental approach
Authors: Richards Jo 
Published: September 2014

SummaryYoung adults and adolescents who experience a first episode of psychosis are faced with important issues and choices concerning their future mental health. Individual and family psychological treatments and psychosocial supportive measures are now well recognised interventions for this group of individuals. This article considers the complexity surrounding medication management after the initial recovery from psychosis. The discussion focuses particularly on the medical treatments, developmental considerations and roles of psychiatrists in improving outcomes for this group of patients.Learning Objectives•Understand the potential benefits and risks of maintenance antipsychotics for young people recovering from first-episode psychosis.•Have an improved knowledge of psychological and developmental concepts that can underpin young people's decision-making regarding maintenance medication for mental illness.•Be able to develop a range of (age-appropriate) strategies to assist medication decision-making.
Application of Ionic Liquid-based Nanofluids Containing Gold Nanoparticles in an Electrochemical Sensor for Uric Acid
Authors: Ding Sufang , Lü Xue , Liu Jie , Xü Ranran 
Published: September 2014

Abstract
Under ultrasonic dispersing, an ionic liquid-based nanofluid was prepared by extraction–reduction method using 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIm][PF6]) as medium, poly(vinylpyrrolidone) as an extractant and a dispersant, and gold nanoparticles (AuNPs) as nanoparticles. A new electrochemical sensor for detecting uric acid (UA) was fabricated by immobilizing the ionic liquid-based nanofluids containing AuNPs on a glassy carbon electrode surface. This sensor exhibited good selectivity, reproducibility, and sensitivity for the direct determination of UA in real human urine samples.
Low-temperature Sintering of Yttria-stabilized Zirconia Using Bismuth–Vanadium Oxide as a Sintering Aid at 800 °C
Authors: Nagao Masahiro , Kobayashi Kazuyo , Hibino Takashi 
Published: September 2014

Abstract
The effect of the addition of copper-doped bismuth–vanadium oxide to yttria-stabilized zirconia (YSZ) on the sinterability and electrical conductivity, especially in reducing atmospheres, has been investigated. When Bi2Cu0.1V0.9O5.35 was used as a sintering aid, the sintering temperature could be reduced to 800 °C, whereas the conventional sintering process requires temperatures above 1300 °C. Although the conductivity of YSZ with 1.5 mol % Bi2Cu0.1V0.9O5.35 was slightly lower than that of pure YSZ sintered at 1300 °C, no PO2 dependence was observed under reducing conditions (e.g., PO2 = 10−5–10−24 atm), which indicates that the electrolyte is applicable to solid-oxide fuel cells.
The immunobiology of cobalt
Authors: Lawrence H. , Deehan D. , Holland J. , Kirby J. , Tyson-Capper A. 
Published: September 2014

Abnormal wear of cobalt-containing metal-on-metal joints is associated with inflammatory pseudotumours. Cobalt ions activate human toll-like receptor 4 (TLR4), which normally responds to bacterial lipopolysaccharide (LPS) in sepsis. Activation of TLR4 by LPS increases the expression of chemokines IL-8 and CXCL10, which recruit leukocytes and activated T-cells, respectively. This study was designed to determine whether cobalt induces a similar inflammatory response to LPS by promoting the expression of IL-8 and CXCL10. A human monocytic cell line, derived from acute monocytic leukaemia, was treated with cobalt ions and expression of IL-8 and CXCL10 measured at mRNA and protein levels. Cobalt-treated macrophages showed a 60-fold increase in IL-8 mRNA, and an eightfold increase in production of the mature chemokine (both p < 0.001); expression of the CXCL10 gene and protein was also significantly increased by cobalt (both p < 0.001). Experiments were also performed in the presence of CLI-095, a TLR4-specific antagonist which abrogated the cobalt-mediated increase in IL-8 and CXCL10 expression. These findings suggest that cobalt ions induce inflammation similar to that observed during sepsis by the simultaneous activation of two TLR4-mediated signalling pathways. These pathways result in increased production of IL-8 and CXCL10, and may be implicated in pseudotumour formation following metal-on-metal replacement. Cite this article: Bone Joint J 2014; 96-B:1172–7.
An anatomical study of the entry point in the greater trochanter for intramedullary nailing
Authors: Farhang K. , Desai R. , Wilber J. H. , Cooperman D. R. , Liu R. W. 
Published: September 2014

Malpositioning of the trochanteric entry point during the introduction of an intramedullary nail may cause iatrogenic fracture or malreduction. Although the optimal point of insertion in the coronal plane has been well described, positioning in the sagittal plane is poorly defined. The paired femora from 374 cadavers were placed both in the anatomical position and in internal rotation to neutralise femoral anteversion. A marker was placed at the apparent apex of the greater trochanter, and the lateral and anterior offsets from the axis of the femoral shaft were measured on anteroposterior and lateral photographs. Greater trochanteric morphology and trochanteric overhang were graded. The mean anterior offset of the apex of the trochanter relative to the axis of the femoral shaft was 5.1 mm (sd 4.0) and 4.6 mm (sd 4.2) for the anatomical and neutralised positions, respectively. The mean lateral offset of the apex was 7.1 mm (sd 4.6) and 6.4 mm (sd 4.6), respectively. Placement of the entry position at the apex of the greater trochanter in the anteroposterior view does not reliably centre an intramedullary nail in the sagittal plane. Based on our findings, the site of insertion should be about 5 mm posterior to the apex of the trochanter to allow for its anterior offset. Cite this article: Bone Joint J 2014;96-B:1274–81.
Industrial ecology a new path to sustainability: a review
Authors: Lyakurwa Felichesmi Selestine 
Published: September 2014

A precise understanding of the link between industrial ecology and sustainability is vitally important for sustainable environmental performances. In this study an intensive review of industrial ecology principles, its application areas and the extent to which industrial ecology has been applied was documented. It was observed that effective application of industrial ecology is critical for sustainability, since the industry is the main polluter of the environment. It was further infered that, there is inadequate application of industrial ecology by developing countries. Thus, I hypothesis that, there is great opportunity for new investment in this field, considering the absence of modern means for the liquid and solid waste management. For example improper incineration of wastes such as hospital wastes and electrical and electronic equipment was perceived to bring health problems in the near future. Thus, it is time for governments, both in developed and developing countries to increase the applicability of industrial ecology, for sustainable social, political, economic and environmental sustainability.
Podocytes: recent biomolecular developments
Authors: Armelloni Silvia , Corbelli Alessandro , Giardino Laura , Li Min , Ikehata Masami , Mattinzoli Deborah , Messa Piergiorgio , Pignatari Chiara , Watanabe Shojiro , Rastaldi Maria Pia 
Published: September 2014

AbstractPodocytes are postmitotic renal glomerular cells with multiple ramifications that extend from the cell body. Processes departing from a podocyte interdigitate with corresponding projections from neighboring cells and form an intricate web that enwraps the glomerular capillary completely. Podocyte processes are interconnected by the slit diaphragm, an adhesion junction mostly formed by Ig-like molecules, cadherins/protocadherins, ephrin/eph, and neurexin molecules organized in an assembly that resembles synaptic junctions. Podocyte failure is primarily or secondarily implicated in all forms of proteinuric glomerular diseases, as confirmed by the morphological changes of their elaborate cell architecture detectable by electron microscopy. Importantly, mutations of podocyte proteins are responsible for the most severe forms of congenital nephrotic syndrome. In the last 15 years, progressive technological advances have aided the study of podocyte biology and pathology, confirming the relevance of podocyte molecules and signaling pathways for the function of the glomerular filter. This review will examine the most important and newest discoveries in the field, which is rapidly evolving, hopefully leading to a detailed knowledge of this fascinating cell and to the development of specific therapeutic options for proteinuric diseases.
Developing Students’ Qualitative Muscles in an Introductory Methods Course
Authors: SmithBattle Lee 
Published: September 2014

AbstractThe exponential growth of qualitative research (QR) has coincided with methodological innovations, the proliferation of qualitative textbooks and journals, and the greater availability of qualitative methods courses. In spite of these advances, the pedagogy for teaching qualitative methods has received little attention. This paper provides a philosophical foundation for teaching QR with active learning strategies and shows how active learning is fully integrated into a one-semester course. The course initiates students into qualitative dispositions and skills as students develop study aims and procedures; enter the field to gather data; analyze the full set of student-generated data; and write results in a final report. Conducting a study in one semester is challenging but has proven feasible and disabuses students of the view that QR is simple, unscientific, or non-rigorous. Student reflections on course assignments are integrated into the paper. The strengths and limitations of this pedagogical approach are also described.
Confusion in <i>Epidendrum brenesii</i> Schltr ., and a new Costa Rican species: <i>Epidendrum sotoanum</i> (Orchidaceae)
Authors: Karremans Adam P. , Hágsater Eric 
Published: September 2014

Two species have been confused under the name Epidendrum brenesii Schltr., both were used in the original description and drawing of the type. The purple-flowered species, has been commonly known as E. brenesii, a specimen collected by Brenes close to the original locus typicus is therefore used to neotypify the species. The yellow-flowered species is described as Epidendrum sotoanum Karremans & Hágsater, in honor of the late Mexican botanist Miguel Ángel Soto Arenas.