bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of
Sporidiobolus salmonicolor
CBS 6832, a Red-Pigmented Basidiomycetous Yeast
Authors: Coelho Marco A. , Almeida João M. G. C. F. , Hittinger Chris Todd , Gonçalves Paula 
Published: May 2015

ABSTRACT

We report the genome sequencing and annotation of the basidiomycetous red-pigmented yeast
Sporidiobolus salmonicolor
strain CBS 6832. The current assembly contains 395 scaffolds, for a total size of about 20.5 Mb and a G+C content of ~61.3%. The genome annotation predicts 5,147 putative protein-coding genes.
Genome Sequence of
Lactococcus lactis
subsp.
cremoris
Mast36, a Strain Isolated from Bovine Mastitis
Authors: Plumed-Ferrer Carme , Gazzola Simona , Fontana Cecilia , Bassi Daniela , Cocconcelli Pier-Sandro , von Wright Atte 
Published: May 2015

ABSTRACT

The genome sequence of
Lactococcus lactis
subsp.
cremoris
Mast36, isolated from bovine mastitis, is reported here. This strain was shown to be able to grow in milk and still possess genes of vegetable origin. The genome also contains a cluster of genes associated with pathogenicity.
Genome Sequence of Mycoplasma meleagridis Type Strain 17529
Authors: Yacoub Elhem , Sirand-Pugnet Pascal , Blanchard Alain , Ben Abdelmoumen Mardassi Boutheina 
Published: May 2015

ABSTRACT

Mycoplasma meleagridis
is a prominent turkey bacterial pathogen associated with airsacculitis and reproductive disorders. Notwithstanding the economic losses caused by
M. meleagridis
, its genome has still not been sequenced. For a better understanding of its genetic background and pathogenicity mechanisms, we sequenced the genome of
M. meleagridis
type strain ATCC 25294.
Genome Assemblies of Three Soil-Associated
Devosia
species:
D. insulae
,
D. limi
, and
D. soli
Authors: Hassan Yousef I. , Lepp Dion , Zhou Ting 
Published: May 2015

ABSTRACT

Agricultural soils constitute highly diverse ecosystems with very rich bacterial populations. Recent studies employing next-generation sequencing techniques have begun to explore the dynamics of bacterial species of such soils and utilized metagenomics approaches to understand how the diversity in soil microorganisms is affected or modified by agricultural practices. Understanding any microorganism's environmental adaptability in the genomic era starts by fully appreciating their encoding genome. Here, we report the draft genome sequences of three
Devosia
species based on three type strains that originated from soil samples:
D. insulae
strain DS-56,
D. limi
strain DSM17137, and
D. soli
strain GH2-10.
Draft Genome Sequence of Aeromonas caviae 8LM, Isolated from Stool Culture of a Child with Diarrhea
Authors: Moriel Bárbara , Cruz Leonardo M. , Dallagassa Cibelle B. , Faoro Helisson , de Souza Emanuel M. , Pedrosa Fábio O. , Rego Fabiane G. M. , Picheth Geraldo , Fadel-Picheth Cyntia M. T. 
Published: May 2015

ABSTRACT

Aeromonas
spp. are Gram-negative rods ubiquitous in aquatic environments; however, some species are able to cause a variety of infections in humans. Here, we report the draft genome sequence of
Aeromonas caviae
8LM isolated from stool culture from a child with diarrhea in southern Brazil.
Complete Genome Sequence of Bacillus subtilis Strain ATCC 6051a, a Potential Host for High-Level Secretion of Industrial Enzymes
Authors: Jeong Haeyoung , Sim Young Mi , Park Seung-Hwan , Choi Soo-Keun 
Published: May 2015

ABSTRACT

Bacillus subtilis
ATCC 6051a (=KCTC 1028), which is less domesticated than strain 168, is widely used for the secretory expression of industrial enzymes. Herein, we present the complete genome sequence of the
Bacillus subtilis
strain ATCC 6051a.
Mood instability is a common feature of mental health disorders and is associated with poor clinical outcomes
Authors: Patel Rashmi , Lloyd Theodore , Jackson Richard , Ball Michael , Shetty Hitesh , Broadbent Matthew , Geddes John R , Stewart Robert , McGuire Philip , Taylor Matthew 
Published: May 2015

ObjectivesMood instability is a clinically important phenomenon but has received relatively little research attention. The objective of this study was to assess the impact of mood instability on clinical outcomes in a large sample of people receiving secondary mental healthcare.DesignObservational study using an anonymised electronic health record case register.SettingSouth London and Maudsley NHS Trust (SLaM), a large provider of inpatient and community mental healthcare in the UK.Participants27 704 adults presenting to SLaM between April 2006 and March 2013 with a psychotic, affective or personality disorder.ExposureThe presence of mood instability within 1 month of presentation, identified using natural language processing (NLP).Main outcome measuresThe number of days spent in hospital, frequency of hospital admission, compulsory hospital admission and prescription of antipsychotics or non-antipsychotic mood stabilisers over a 5-year follow-up period.ResultsMood instability was documented in 12.1% of people presenting to mental healthcare services. It was most frequently documented in people with bipolar disorder (22.6%), but was common in people with personality disorder (17.8%) and schizophrenia (15.5%). It was associated with a greater number of days spent in hospital (β coefficient 18.5, 95% CI 12.1 to 24.8), greater frequency of hospitalisation (incidence rate ratio 1.95, 1.75 to 2.17), greater likelihood of compulsory admission (OR 2.73, 2.34 to 3.19) and an increased likelihood of prescription of antipsychotics (2.03, 1.75 to 2.35) or non-antipsychotic mood stabilisers (2.07, 1.77 to 2.41).ConclusionsMood instability occurs in a wide range of mental disorders and is not limited to affective disorders. It is generally associated with relatively poor clinical outcomes. These findings suggest that clinicians should screen for mood instability across all common mental health disorders. The data also suggest that targeted interventions for mood instability may be useful in patients who do not have a formal affective disorder.
Relationship between low Ankle-Brachial Index and rapid renal function decline in patients with atrial fibrillation: a prospective multicentre cohort study
Authors: Violi Francesco , Pastori Daniele , Perticone Francesco , Hiatt William R , Sciacqua Angela , Basili Stefania , Proietti Marco , Corazza Gino R , Lip Gregory Y H , Pignatelli Pasquale ,   in collaboration with the ARAPACIS (Atrial Fibrillation Registry for Ankle-Brachial Index Prevalence Assessment-Collaborative Italian Study) STUDY group
Published: May 2015

ObjectiveTo investigate the relationship between Ankle-Brachial Index (ABI) and renal function progression in patients with atrial fibrillation (AF).DesignObservational prospective multicentre cohort study.SettingAtherothrombosis Center of I Clinica Medica of ‘Sapienza’ University of Rome; Department of Medical and Surgical Sciences of University Magna Græcia of Catanzaro; Atrial Fibrillation Registry for Ankle-Brachial Index Prevalence Assessment-Collaborative Italian Study.Participants897 AF patients on treatment with vitamin K antagonists.Main outcome measuresThe relationship between basal ABI and renal function progression, assessed by the estimated Glomerular Filtration Rate (eGFR) calculated with the CKD-EPI formula at baseline and after 2 years of follow-up. The rapid decline in eGFR, defined as a decline in eGFR >5 mL/min/1.73 m2/year, and incident eGFR<60 mL/min/1.73 m2were primary and secondary end points, respectively.ResultsMean age was 71.8±9.0 years and 41.8% were women. Low ABI (ie, ≤0.90) was present in 194 (21.6%) patients. Baseline median eGFR was 72.7 mL/min/1.73 m2, and 28.7% patients had an eGFR<60 mL/min/1.73 m2. Annual decline of eGFR was −2.0 (IQR −7.4/−0.4) mL/min/1.73 m2/year, and 32.4% patients had a rapid decline in eGFR. Multivariable logistic regression analysis showed that ABI ≤0.90 (OR 1.516 (95% CI 1.075 to 2.139), p=0.018) and arterial hypertension (OR 1.830 95% CI 1.113 to 3.009, p=0.017) predicted a rapid eGFR decline, with an inverse association for angiotensin converting enzyme (ACE) inhibitors/angiotensin receptor blockers (OR 0.662 95% CI 0.464 to 0.944, p=0.023). Among the 639 patients with AF with eGFR >60 mL/min/1.73 m2, 153 (23.9%) had a reduction of the eGFR <60 mL/min/1.73 m2. ABI ≤0.90 was also an independent predictor for incident eGFR<60 mL/min/1.73 m2(HR 1.851, 95% CI 1.205 to 2.845, p=0.005).ConclusionsIn patients with AF, an ABI ≤0.90 is independently associated with a rapid decline in renal function and incident eGFR<60 mL/min/1.73 m2. ABI measurement may help identify patients with AF at risk of renal function deterioration.Trial registration numberNCT01161251.
High value cost conscious care for diabetic patients at a community hospital
Authors: Karu Eric , Shahnazarian Vahe , Mehta Parag 
Published: May 2015

AbstractAt New York Methodist Hospital (Brooklyn, NY), the pattern of ordering glucose testing was studied by a multidisciplinary committee because the medicine residents were placing inpatient chemstrip orders at their own discretion. It was found that chemstrip orders were being placed at inappropriate frequencies, and occasionally on inappropriate patients.The staff and residents were educated on daily rounds in order to achieve the goal of reducing unwarranted fingersticks, consequently increasing patient satisfaction and reducing wasted time, resources, and costs. From April 2014 through March 2015 there were 274,889 fingersticks in the inpatient setting and following the intervention the number of fingersticks had decreased to 238,187, representing a significant decrease.
The Role of TLR4 in Chemotherapy-Driven Metastasis
Authors: Ran Sophia 
Published: May 2015

AbstractTumor resistance to cytotoxic drugs is one of the main obstacles to successful cancer therapy. Emerging evidence suggests that chemoresistance is promoted by substances released from dead and damaged cells that activate the host repair program orchestrated by Toll-like receptor-4 (TLR4). TLR4 is often overexpressed in malignant and tumor-infiltrating immune cells. In addition to endogenous ligands released by therapy-induced tumor destruction, TLR4 is directly activated by paclitaxel, one of the most commonly used chemotherapeutic drugs against various human cancers. TLR4 activation promotes local and systemic inflammation, leading to induction of multiple circuits that create a regenerative environment favoring local recurrence and metastasis. Of particular importance is TLR4-mediated recruitment of endothelial progenitors derived from immature myeloid cells. These cells play a major role in rebuilding tumor-associated lymphatic and blood vessels, thereby promoting lymphatic and hematogenous metastasis. The latter is further enhanced by the premetastatic niche generated by mobilization of myeloid provascular cells to distant organs. This review summarizes the recent evidence demonstrating that paclitaxel and other clinically used anticancer drugs actively induce metastasis even while shrinking the primary tumor. Better understanding of the mechanisms underlying TLR4-dependent chemotherapy-driven metastasis might be the key to overcoming challenges of cancer eradication. Cancer Res; 75(12); 2405–10. ©2015 AACR.
Intratumoral CD14+ Cells and Circulating CD14+HLA-DRlo/neg Monocytes Correlate with Decreased Survival in Patients with Clear Cell Renal Cell Carcinoma
Authors: Gustafson Michael P. , Lin Yi , Bleeker Jonathan S. , Warad Deepti , Tollefson Matthew K. , Crispen Paul L. , Bulur Peggy A. , Harrington Susan M. , Laborde Rebecca R. , Gastineau Dennis A. , Leibovich Bradley C. , Cheville John C. , Kwon Eugene D. , Dietz Allan B. 
Published: May 2015

Abstract
Purpose: Immunotherapeutic strategies to treat patients with renal cell carcinoma (RCC) offer new opportunities for disease management. Further improvements to immunotherapy will require additional understanding of the host response to RCC development.
Experimental Design: Using a novel approach to understanding the immune status of cancer patients, we previously showed that patients with a certain immune profile had decreased overall survival. Here, we examine in more detail the phenotypic changes in peripheral blood and the potential consequences of these changes in RCC patients.
Results: We found that CD14+HLA-DRlo/neg monocytes were the most predominant phenotypic change in peripheral blood of RCC patients, elevated nearly 5-fold above the average levels measured in healthy volunteers. Intratumoral and peritumoral presence of CD14 cells was an independent prognostic factor for decreased survival in a cohort of 375 RCC patients. The amount of peripheral blood CD14+HLA-DRlo/neg monocytes was found to correlate with the intensity of CD14 staining in tumors, suggesting that the measurement of these cells in blood may be a suitable surrogate for monitoring patient prognosis. The interaction of monocytes and tumor cells triggers changes in both cell types with a loss of HLA-DR expression in monocytes, increases of monocyte survival factors such as GM-CSF in tumors, and increased production of angiogenic factors, including FGF2.
Conclusions: Our results suggest a model of mutually beneficial interactions between tumor cells and monocytes that adversely affect patient outcome. Clin Cancer Res; 21(18); 4224–33. ©2015 AACR.
A Phase I Trial of the Anti-KIR Antibody IPH2101 and Lenalidomide in Patients with Relapsed/Refractory Multiple Myeloma
Authors: Benson Don M. , Cohen Adam D. , Jagannath Sundar , Munshi Nikhil C. , Spitzer Gary , Hofmeister Craig C. , Efebera Yvonne A. , Andre Pascale , Zerbib Robert , Caligiuri Michael A. 
Published: May 2015

Abstract
Purpose: Natural killer (NK) cells may play an important role in the immune response to multiple myeloma; however, multiple myeloma cells express killer immunoglobulin-like receptor (KIR) ligands to prevent NK cell cytotoxicity. Lenalidomide can expand and activate NK cells in parallel with its direct effects against multiple myeloma; however, dexamethasone may impair these favorable immunomodulatory properties. IPH2101, a first-in-class antiinhibitory KIR antibody, has acceptable safety and tolerability in multiple myeloma as a single agent. The present work sought to characterize lenalidomide and IPH2101 as a novel, steroid-sparing, dual immune therapy for multiple myeloma.
Experimental Design: A phase I trial enrolled 15 patients in three cohorts. Lenalidomide was administered per os at 10 mg on cohort 1 and 25 mg on cohorts 2 and 3 days 1 to 21 on a 28-day cycle with IPH2101 given intravenously on day 1 of each cycle at 0.2 mg/kg in cohort 1, 1 mg/kg in cohort 2, and 2 mg/kg in cohort 3. No corticosteroids were utilized. The primary endpoint was safety, and secondary endpoints included clinical activity, pharmacokinetics (PK), and pharmacodynamics (PD).
Results: The biologic endpoint of full KIR occupancy was achieved across the IPH2101 dosing interval. PD and PK of IPH2101 with lenalidomide were similar to data from a prior single-agent IPH2101 trial. Five serious adverse events (SAE) were reported. Five objective responses occurred. No autoimmunity was seen.
Conclusions: These findings suggest that lenalidomide in combination with antiinhibitory KIR therapy warrants further investigation in multiple myeloma as a steroid-sparing, dual immune therapy. This trial was registered at www.clinicaltrials.gov (reference: NCT01217203). Clin Cancer Res; 21(18); 4055–61. ©2015 AACR.
PTK7
+
Mononuclear Cells Express VEGFR2 and Contribute to Vascular Stabilization by Upregulating Angiopoietin-1
Authors: Chauhan Sunil K. , Lee Hyung Keun , Lee Hyun Soo , Park Eun Young , Jeong Eunae , Dana Reza 
Published: May 2015

Objective—
In angiogenesis, circulating mononuclear cells are recruited to vascular lesions; however, the underlying mechanisms are poorly understood.

Approach and Results—

Here, we characterize the functional role of protein tyrosine kinase 7 (PTK7)–expressing CD11b
+
mononuclear cells in vitro and in vivo using a mouse model of angiogenesis. Although the frequencies of PTK7
+
CD11b
+
cells in the bone marrow remained similar after vascular endothelial growth factor-A–induced neovascularization, we observed an 11-fold increase in the cornea. Importantly, vascular endothelial growth factor-A–induced chemotaxis of PTK7
+
cells was mediated by vascular endothelial growth factor receptor 2. In a coculture with endothelial cells, PTK7
+
CD11b
+
cells stabilized the vascular network for 2 weeks by expressing high levels of angiopoietin-1. The enhanced vascular stability was abolished by knockdown of angiopoietin-1 in PTK7
+
CD11b
+
cells and could be restored by angiopoietin-1 treatment.

Conclusions—
We conclude that PTK7 expression in perivascular mononuclear cells induces vascular endothelial growth factor receptor 2 and angiopoietin-1 expression and thus contributes to vascular stabilization in angiogenesis.
Spontaneous Aortic Regurgitation and Valvular Cardiomyopathy in Mice
Authors: Hajj Georges P. , Chu Yi , Lund Donald D. , Magida Jason A. , Funk Nathan D. , Brooks Robert M. , Baumbach Gary L. , Zimmerman Kathy A. , Davis Melissa K. , El Accaoui Ramzi N. , Hameed Tariq , Doshi Hardik , Chen BiYi , Leinwand Leslie A. , Song Long-Sheng , Heistad Donald D. , Weiss Robert M. 
Published: May 2015

Objective—
We studied the mechanistic links between fibrocalcific changes in the aortic valve and aortic valve function in mice homozygous for a hypomorphic epidermal growth factor receptor mutation (Wave mice). We also studied myocardial responses to aortic valve dysfunction in Wave mice.

Approach and Results—
At 1.5 months of age, before development of valve fibrosis and calcification, aortic regurgitation, but not aortic stenosis, was common in Wave mice. Aortic valve fibrosis, profibrotic signaling, calcification, osteogenic markers, lipid deposition, and apoptosis increased dramatically by 6 and 12 months of age in Wave mice. Aortic regurgitation remained prevalent, however, and aortic stenosis was rare, at all ages. Proteoglycan content was abnormally increased in aortic valves of Wave mice at all ages. Treatment with pioglitazone prevented abnormal valve calcification, but did not protect valve function. There was significant left ventricular volume overload, hypertrophy, and fetal gene expression, at all ages in Wave mice with aortic regurgitation. Left ventricular systolic function was normal until 6 months of age in Wave mice, but became impaired by 12 months of age. Myocardial transverse tubules were normal in the presence of left ventricular hypertrophy at 1.5 and 3 months of age, but became disrupted by 12 months of age.

Conclusions—
We present the first comprehensive phenotypic and molecular characterization of spontaneous aortic regurgitation and volume-overload cardiomyopathy in an experimental model. In Wave mice, fibrocalcific changes are not linked to valve dysfunction and are epiphenomena arising from structurally incompetent myxomatous valves.
Racial Differences in Circulating Natriuretic Peptide Levels: The Atherosclerosis Risk in Communities Study
Authors: Gupta Deepak K. , Claggett Brian , Wells Quinn , Cheng Susan , Li Man , Maruthur Nisa , Selvin Elizabeth , Coresh Josef , Konety Suma , Butler Kenneth R. , Mosley Thomas , Boerwinkle Eric , Hoogeveen Ron , Ballantyne Christie M. , Solomon Scott D. 
Published: May 2015

Background

Natriuretic peptides promote natriuresis, diuresis, and vasodilation. Experimental deficiency of natriuretic peptides leads to hypertension (
HTN
) and cardiac hypertrophy, conditions more common among African Americans. Hospital‐based studies suggest that African Americans may have reduced circulating natriuretic peptides, as compared to Caucasians, but definitive data from community‐based cohorts are lacking.

Methods and Results

We examined plasma N‐terminal pro B‐type natriuretic peptide (
NT
pro
BNP
) levels according to race in 9137 Atherosclerosis Risk in Communities (
ARIC
) Study participants (22% African American) without prevalent cardiovascular disease at visit 4 (1996–1998). Multivariable linear and logistic regression analyses were performed adjusting for clinical covariates. Among African Americans, percent European ancestry was determined from genetic ancestry informative markers and then examined in relation to
NT
pro
BNP
levels in multivariable linear regression analysis.
NT
pro
BNP
levels were significantly lower in African Americans (median, 43 pg/
mL
; interquartile range [
IQR
], 18, 88) than Caucasians (median, 68 pg/
mL
;
IQR
, 36, 124;
P
<0.0001). In multivariable models, adjusted log
NT
pro
BNP
levels were 40% lower (95% confidence interval [
CI
], −43, −36) in African Americans, compared to Caucasians, which was consistent across subgroups of age, gender,
HTN
, diabetes, insulin resistance, and obesity. African‐American race was also significantly associated with having nondetectable
NT
pro
BNP
(adjusted
OR
, 5.74; 95%
CI
, 4.22, 7.80). In multivariable analyses in African Americans, a 10% increase in genetic European ancestry was associated with a 7% (95%
CI
, 1, 13) increase in adjusted log
NT
proBNP.

Conclusions

African Americans have lower levels of plasma
NT
pro
BNP
than Caucasians, which may be partially owing to genetic variation. Low natriuretic peptide levels in African Americans may contribute to the greater risk for
HTN
and its sequalae in this population.
Infratentorial Microbleeds
Authors: Arkink Enrico B. , Terwindt Gisela M. , de Craen Anton J.M. , Konishi Junya , van der Grond Jeroen , van Buchem Mark A. , Ferrari Michel D. , Kruit Mark C. 
Published: May 2015

Background and Purpose—
Migraine is a risk factor for clinical stroke and for subclinical white matter hyperintensities and infratentorial infarcts. These subclinical lesions are linked to small-vessel pathology. Cerebral microbleeds (CMBs) are another biomarker of small-vessel disease but have not yet been studied in migraine.

Methods—
Identification of CMBs in 63 migraineurs (25 with aura/35 without aura/3 unknown aura status) and 359 controls (aged, 73–85 years) from the Prospective Study of Pravastatin in the Elderly at Risk (PROSPER) magnetic resonance imaging study. We assessed the modifying role of migraine in the co-occurrence of CMBs, infarcts, and white matter hyperintensity-load.

Results—
Infratentorial microbleeds were more prevalent in migraine without aura patients than controls (14% versus 4%). Prevalence of other CMBs, infarcts, and white matter hyperintensities did not differ between groups. Migraineurs with CMBs had more often infarcts than controls with CMBs (65% versus 43%). In comparison with controls with infarcts, migraineurs with infarcts had more commonly CMBs (55% versus 30%).

Conclusions—
Migraine, notably without aura, is associated with infratentorial CMBs at older age. CMBs and infarcts co-occur more often in migraine than in controls. This supports the hypothesis of small-vessel involvement in migraine pathophysiology.
Faiths from the Archipelago
Authors: Majeri Mangunjaya Fachruddin , Tobing Imran S.L. , Binawan Andang , Pua Evangeline , Nurbawa Made 
Published: May 2015

This paper explores the responses of faith-based movements in Indonesia to globalized environmental issues, including climate change. As a plural nation with six official religions, the leaders of the major recognized religions in Indonesia (i.e., Islam, Protestant and Roman Catholic Christianity, Hinduism, Buddhism and Confusianism) all offered statements in 2007 at COP 13, the international forum in response to the environmental challange and climate change in Bali. Each called for religious adherents to act in order to contribute to halting the problem of climate change. Faith is seen to be a key factor in motivating people to change their behavior towards the environment. The slogan, “Think globally, act locally,” is at the heart of this commitment, in hopes that environmental challenges including climate change, become a common issue among all the religions in Indonesia, and so that Indonesian responses may be collaborative and cooperative. This paper will document and analyze the development of such faith-based action with respect to environmental challenges in Indonesia, in light of global conditions.