bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of the angiotensinogen gene polymorphism with atherosclerosis and its risk traits in the Saudi population
Authors: Al-Najai Mohammed , Muiya Paul , Tahir Asma I , Elhawari Samar , Gueco Daisy , Andres Editha , Mazhar Nejat , Altassan Nada , Alshahid Maie , Dzimiri Nduna 
Published: March 2013

Abstract

Background
Angiotensinogen (AGT) constitutes a central component of the renin-angiotensin system that controls the systemic blood pressure and several other cardiovascular functions and may play an important role in atherosclerosis pathways. In this study, we employed TaqMan genotyping assays to evaluate the role of 8 AGT variants in primary hypertension (HTN), type 2 diabetes mellitus (T2DM), and obesity as a possible trigger of coronary artery disease (CAD) in a population of 4615 angiographed native Saudi individuals.

Methods
Linkage analysis was done by using the Affymetrix Gene Chip array, sequencing by using the MegaBACE DNA analysis system and genotyping accomplished by TaqMan chemistry using the Applied Biosystem real-time Prism 7900HT Sequence Detection System.

Results
Six variants, rs2067853 GG [Odds ratio(95% Confidence Interval) = 1.44(1.17-1.78); p = 0.001], rs7079 [1.49(1.20-1.85); p < 0.0001], rs699 G [1.19(1.08-1.13); p < 0.0001], rs3789679 A [1.51(1.14-1.99); p = 0.004], rs2148582 GG [1.31(1.11-1.55); p = 0.002] and rs5051 TC + CC [1.32(1.13-1.60); p = 0.001] conferred risk for HTN (3521 cases versus 1094 controls). The rs2067853 (p = 0.042), rs699G (p = 0.007) and rs5051 (p = 0.051) also conferred risk for myocardial infarction (MI; 2982 vs 1633), while rs3789679 A (p < 0.0001) and GA + AA (p < 0.0001) as well as rs4762G (p = 0.019) were associated with obesity (1576 vs 2458). However, while these variants appeared to be also associated with CAD (2323 vs 2292), only the rs7079G (p = 0.035) retained its significant relationship. Interestingly, among the haplotypes constructed from these SNPs, the baseline 8-mer haplotype, GGTGGGGT (χ2 = 7.02; p = 0.0081) and another GGCGGAGT (χ2 = 5.10; p = 0.024), together with several of their derivatives were associated with HTN. T2DM was associated with two 8-mer haplotypes, GGTAGGAC (χ2 = 5.66; p = 0.017) and ATTGAGAC (χ2 = 5.93; p = 0.015), obesity with GGCGGAGT (χ2 = 9.49; p = 0.0021) and MI was linked to ATTGGGAC (χ2 = 6.68; p = 0.010) and GGTGGGAT (χ2 = 4.25; p = 0.039). Furthermore, several causative haplotypes were also shared among the risk traits as well as with CAD.

Conclusion
These results point to AGT as independently conferring risk for various cardiovascular traits, and possibly interacting with these traits in events leading to atherosclerosis.
Neonatal extravasation injury: prevention and management in Australia and New Zealand-a survey of current practice
Authors: Restieaux Matthew , Maw Andrew , Broadbent Roland , Jackson Pam , Barker David , Wheeler Ben 
Published: March 2013

Abstract

Background
Extravasation injury remains an important cause of iatrogenic injury in neonatal intensive care. This study aims to describe the current approach to extravasation injury (EI) prevention and management in Neonatal Intensive Care Units (NICUs) in Australia and New Zealand.

Methods
A literature review regarding extravasation injury in the newborn was carried out to inform questionnaire design. An internet-based survey was then conducted with the clinical directors of the 27 tertiary NICUs in Australia and New Zealand.

Results
The survey received a 96% response rate. Approximately two thirds of Australian and New Zealand NICUs have written protocols for prevention and management of extravasation injury. Considerable practice variation was seen for both prevention and treatment of EI. 92% of units had experienced cases of significant EI.

Conclusions
Australian and New Zealand tertiary neonatal units clearly recognise EI as an important cause of iatrogenic morbidity and mortality. Significant variation still exists among units with regards to guidelines for both prevention and management of EI. We recommend that neonatal staff should remain vigilant, ensuring that guidelines for the prevention and treatment of EI are available, and rigorously followed.
Aggressive natural killer-cell leukemia with jaundice and spontaneous splenic rupture: a case report and review of the literature
Authors: Gao Li-min , Liu Wei-ping , Yang Qun-pei , Li Hui-fang , Chen Jun-jie , Tang Yuan , Zou Yan , Liao Dian-Ying , Liu Yan-mei , Zhao Sha 
Published: March 2013

Abstract
Aggressive natural killer cell leukemia/lymphoma (ANKL) is a rare aggressive form of NK-cell neoplasm. We report an uncommon case of 36-year-old male who showed jaundice and spontaneous splenic rupture. The diagnosis was established by the biopsy of liver and spleen. The monomorphous medium-size neoplastic cells infiltrated into portal areas and sinus of liver as well as the cords and sinus of the spleen. Necrosis, mitotic figures and significant apoptosis could be seen easily. These neoplastic cells demonstrated a typical immunophenotype of CD3ε+, CD56+, CD16+, Granzyme B+, TIA-1+. T-cell receptor γ (TCR-γ) gene rearrangement analysis showed germline configuration and the result of in situ hybridization for Epstein-Barr virus-encoded RNA (EBER-ISH) was positive. The patient has undergone an aggressive clinical course and died of multi-organ function failure 14 days later after admission. To the best of our knowledge, this is the first case of ANKL with spontaneous splenic rupture, and we should pay more attention to recognize it.

Virtual Slides

The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/2048154883890867
SVAw - a web-based application tool for automated surrogate variable analysis of gene expression studies
Authors: Pirooznia Mehdi , Seifuddin Fayaz , Goes Fernando S , Leek Jeffrey T , Zandi Peter P 
Published: March 2013

Abstract

Background
Surrogate variable analysis (SVA) is a powerful method to identify, estimate, and utilize the components of gene expression heterogeneity due to unknown and/or unmeasured technical, genetic, environmental, or demographic factors. These sources of heterogeneity are common in gene expression studies, and failing to incorporate them into the analysis can obscure results. Using SVA increases the biological accuracy and reproducibility of gene expression studies by identifying these sources of heterogeneity and correctly accounting for them in the analysis.

Results
Here we have developed a web application called SVAw (Surrogate variable analysis Web app) that provides a user friendly interface for SVA analyses of genome-wide expression studies. The software has been developed based on open source bioconductor SVA package. In our software, we have extended the SVA program functionality in three aspects: (i) the SVAw performs a fully automated and user friendly analysis workflow; (ii) It calculates probe/gene Statistics for both pre and post SVA analysis and provides a table of results for the regression of gene expression on the primary variable of interest before and after correcting for surrogate variables; and (iii) it generates a comprehensive report file, including graphical comparison of the outcome for the user.

Conclusions
SVAw is a web server freely accessible solution for the surrogate variant analysis of high-throughput datasets and facilitates removing all unwanted and unknown sources of variation. It is freely available for use at http://psychiatry.igm.jhmi.edu/sva. The executable packages for both web and standalone application and the instruction for installation can be downloaded from our web site.
Malignancies after kidney transplantation: 3 clinical case reports
Authors: Dalinkevičienė Eglė , Kuzminskis Vytautas , Kairevičė Laura , Jančiauskienė Rasa , Milonas Daimantas , Jievaltas Mindaugas , Sribikienė Birutė , Bumblytė Inga-Arūnė 
Published: March 2013

Abstract
Post-transplant malignancies present an aggressive course and are a significant cause of morbidity and mortality. Tumours of viral ethiology have the greatest risk in renal transplant recipients. Oncogenic effect of immunosuppressive therapy is another major risk factor of post-transplant malignancy. We report cases of three different types of malignancies developed after kidney transplantation: non-Hodgkin’s lymphoma, Kaposi’s sarcoma and germ cell testicular cancer (nonseminoma).
Research and Development and Tax Incentives
Authors: Hodžić Sabina 
Published: March 2013

Abstract
In many countries, tax incentives are a popular means to achieve political, economic and social objectives. Their aim is to reach and accelerate certain activities of public interest. Furthermore, one of the objectives is to accelerate the development of a certain industry and influence the growth of research and investment in foreign capital. Innovation is the key element that helps a company achieve competitive advantage. Global competition is forced to offer unique products with added values on the market. Tax incentives for research and development are an important factor of innovation. This paper aims to present the importance of research and development, as well as the role of tax incentives. States should use their fiscal policy to stimulate investment in research and development through various forms of tax relief. The Republic of Croatia applies tax incentives for research and development, but to a significantly less extent than other European Union countries.
The Influence of Globalization in Promoting Entrepreneurship in Malaysia
Authors: Arokiasamy Anantha Raj A. 
Published: March 2013

Abstract
Entrepreneurship is increasingly becoming a very relevant instrument in promoting economic growth in a country. Thus the role of globalization in promoting entrepreneurship in Malaysia is analyzed in this context. In this regard, universities as teaching and research units and according to their potential and capabilities play an important role in development, entrepreneurship and job creation. What affect does globalization have on an economy? Globalization of education and entrepreneurial studies has allowed efficient access to the scientific environment and culture of various countries. Given the ever-changing world in which we live, this paper aims to explain the importance of investigating educational and research activities of universities converging towards globalization and entrepreneurship.
Supply Management: A Transaction Cost Economics Framework
Authors: Garfamy Reza Mohammady 
Published: March 2013

Abstract
Transaction Cost Economics (TCE) is an economic theory that provides an analytical framework for investigating the governance structure of contractual relations within a supply chain. The purpose of this paper is to examine existing research in an effort to understand the potential effects of transaction costs on the vertical coordination of a supply chain. The paper arrives at many insights into how supply chains are organized under different governance structures. These insights can certainly be shared via the development and introduction of related propositions. The conceptual typology of contractual relations developed herein can help researchers better understand the scope of both the problems and the opportunities associated with supply management. It will be of value, therefore, not only to researchers who desire to expand their research into this area, but also to those who have already investigated this topic in isolation or with limited scope.
On the Ethnographic Categorization of Biodiversity in the Danube Delta “Biosphere Reserve”
Authors: Constantin Marin 
Published: March 2013

Abstract
This paper is an interpretative approach of a conflicting state of affairs in the Danube Delta, Romania, where local communities of Russian and Ukrainian fishermen claim their “rights” of wetland management in the context of (and sometimes against) the government policies of “conserving biodiversity”. My general assumption is that divergences acting between fishermen and the authorities in the Danube Delta are essentially rooted in a kind of “generalized (but not insurmountable) incongruity” of two such occupational and institutional “worlds” and, moreover “worldviews”.
The Business Media for the Rural World – an Analysis on the Romanian Audiovisual
Authors: Radu Raluca Nicoleta 
Published: March 2013

Abstract
Starting from the findings a Romanian research group gathered during the project “Models of mass-media consumption in the Romanian rural world: traditional identities, European identities” of the Faculty of Journalism and Communication Studies, University of Bucharest, this article identifies some risk factors in the conceptual design of an audiovisual programme for the rural world. These factors are: (1) differences in the definitions of the public - in our case, the Romanian peasant - as they are held by the journalist, the programme editor, the sources and by the public itself; (2) a quasi-general lack of vision about the future of the rural world, based on a lack of information; and (3) deficiency in applying journalistic information gathering techniques and text writing rules. The conclusions are based on media product analysis of business journalism programmes broadcast by the Romanian public broadcasting services for rural audiences and on questionnaire-based and in-depth interviewing with potential members of the public.
Blood dendritic cells in cattle infected with bovine leukemia virus (BLV): isolation and phenotyping
Authors: Szczotka M. , Kuźmak J. , Kostro K. , Bednarek D. , Purzycka M. 
Published: March 2013

AbstractDendritic cells (DCs) are most potent antigen presenting cells (APCs) with unique ability to prime effective immune responses. They express higher levels of MHC class II and accesory molecules on their surface, than other professional APCs. The investigations were performed on DCs generated from blood with the use of microbeads magnetically labeled with mouse anti human CD14. Flow cytometry was applied for determination of DCs immunophenotype in healthy and naturally infected with BLV cattle. For immunophenotyping mouse monoclonal antibodies anti bovine: CD11a, CD11b, CD11c, MHC-I and MHC-II were used. Our results demonstrated that dendritic cells infected with BLV expressed very high percentage of determinants: CD11a, CD11b, CD11c, MHC-I and MHC-II class. Leukaemic DCs exhibited DCs morphology and had a phenotype of mature DCs. The expression of gp51 glycoprotein of BLV on leukaemic DCs was detected in flow cytometry investigations.
Effect of a two-week treatment with low dose of ortho-substituted polychlorinated biphenyls (PCB104 and PCB153) on VEGF-receptor system expression in the choroid plexus in adult ewes
Authors: Szczepkowska A. , Młynarczuk J. , Grochowalski A. , Dufourny L. , Thiéry J.-C. , Skipor J. 
Published: March 2013

AbstractOrtho-substituted polychlorinated biphenyl (PCB) congeners, which constitute a large part of PCB residues found in the environment and in animal tissues, are known to exert potent vascular effects and can activate endothelial cells in the periphery and in the brain. The choroid plexus (CP) is responsible for cerebrospinal fluid (CSF) production and its epithelial cell layer is responsible for structure and functions of the blood-CSF barrier. The aims of this study were: 1) to investigate if environmentally relevant doses of PCB153 and similar doses of PCB104 caused changes in the expression of vascular endothelial growth factor (VEGF) - receptor system, which maintains CP function, and 2) to determine the level of both congeners in blood plasma after their oral administration. Studies of both congeners were performed on ovariectomized ewes treatedper oswith low doses (0.1 mg/kg, three times a week for two weeks) of PCB153 (n=4) or PCB104 (n=4) and vehicle (control, n=4). The effects of PCB153 and PCB104 treatment on mRNA expression of two isoforms of VEGF (VEGF120 and VEGF164) and their receptors Flt-1 and KDR were determined using real-time PCR. Plasma concentration of PCBs was measured using high resolution chromatography/tandem mass spectrometry (HRGC/MS-MS). We observed that neither PCB153 nor PCB104 significantly altered the mRNA of the VEGF-receptor system in the CP. In PCB treated animals plasma concentration of PCB153 (1.425 ± 0.16 ng/g of dry mass, DM) was about 150 times higher than PCB104 (0.009 ± 0.007ng/g DM). In control animals the PCB153 level was 0.14 ± 0.031 ng/g DM, while the PCB104 level was below detection level. This indicates that increase in plasma PCB153 concentration to levels similar to those reported in humans and of PCB104 concentration to levels 100 times higher than those found in human plasma did not affect the VEGF-receptor system in the CP in adult ewes. The significantly lower increase of PCB104 than PCB153 concentration in blood after oral administration suggests different absorption of both congeners from the digestive tract.
Mismatch between transcriptomic and histopathologic picture of canine lymphomas
Authors: Ostrzeszewicz M. , Maciejewski H. , Sapierzyński R. , Micuń J. , Majewska A. , Lechowski R. , Motyl T. , Jank M. 
Published: March 2013

Abstract
Lymphoma is one of the most common malignant tumours occurring in dogs. Since there is a constant need for new, more comprehensive laboratory diagnostic tools which permit the precise determination of many tumour-related factors we decided to verify whether the use of microarray analysis could be helpful in classifying lymphomas. The study was performed on samples collected from 7 dogs in which multicentric lymphoma was recognized. Among this group we were able to identify one sub-cluster of transcriptionally similar tumours, which completely differed in terms of the histopathological examination. Among them there were one diffuse large B cell lymphoma, one diffuse macronucleolated medium-sized cell lymphoma and one pleomorphic mixed small and large T-cell lymphoma. The lymphomas belonging to the sub-cluster differed from other analysed tumours in the expression of more than 100 genes of which only 18 were described earlier in regard to lymphomas.
« L'invention » de l'immigré. La psychiatrie belge face à la migration maghrébine dans les années 1960 et 1970
Authors: Majerus Benoît , Richel Julie 
Published: March 2013

L’article croise histoire de l’immigration et histoire de la psychiatrie en s’intéressant à la façon dont une partie de la médecine a contribué à la fabrication de « l’immigré » en Belgique dans les années 1960-1970. À partir de dossiers médicaux, il montre que l’appréhension dominante s’est faite en termes culturalistes, le concept de culture servant de conceptcoupole qui permet d’articuler plusieurs différences difficilement appréhendables par les psychiatres. La culture peut servir pour analyser des rôles et des conflits interfamiliaux, expliquer les conflits entre pays d’émigration et pays d’immigration et exprimer l’incompréhension entre psychiatre et patient.
Coping Strategies for Child Bully-Victims
Authors: Buxton David , Potter Mona Patel , Bostic Jeff Q. 
Published: March 2013

CME Educational Objectives

1.
Distinguish what types of bullying are most common at different ages.

2.
Describe six questions to screen for bullying and its effects.

3.
Identify three interventions for students and parents to address bullying, victimization, bystanding, and system rules that allow bullying.

Bullying affects most children in some way, as victims, bystanders, or bullies, during childhood. Children exposed to bullying may present with physical as well as psychological symptoms, and experience negative effects lasting into adulthood. This article provides information regarding bullying screening and interventions with pediatric and adolescent patients. Taking into account a child’s developmental stage, age-appropriate tactics are provided to address bullying, victimization, bystanding, and system “rules” that tolerate or foster bullying.
A Comparison of the Niagara™ and Dolphin® Catheters for Continuous Renal Replacement Therapy
Authors: Kim Inbyung , Fealy Nigel , Baldwin Ian , Bellomo Rinaldo 
Published: March 2013

Purpose The choice of vascular access catheter may affect filter life during continuous renal replacement therapy (CRRT); specifically, a new surface-modified catheter has been reported to possibly prevent thrombosis and catheter malfunction. Design and Setting A sequential, controlled study in a tertiary ICU. Aims To compare circuit life when CRRT was performed with a Bard®Niagara™ catheter or the surface-modified GamCath™ Dolphin® Protect 1320 catheter. Patients and measurements We studied 50 patients with acute kidney injury requiring CRRT, all delivered with catheters in the femoral position. We obtained information on age, gender, disease severity score (APACHE II and APACHE III), filter life, total heparin dose, hemoglobin concentration, platelet count, INR, and aPTT during CRRT. Results We studied 341 circuits in 50 patients; 30 patients (140 circuits) used the Niagara and 20 patients (201 circuits) used the Dolphin catheter. Mean of circuit life in two groups was 14.9 hours and 13.1 hours, respectively (p=0.22). Patients using Niagara catheters had a more prolonged APTT (p<0.01) and lower platelet count (p=0.05), while heparin dose (p=0.22), and other anticoagulant treatment (p=0.73) were not significantly different. On Kaplan-Meier survival analysis, circuit life was not significant different between the two catheters (p=0.15). Conclusions The Niagara and Dolphin catheters appear to be broadly equivalent in terms of their impact on circuit life.
Growth-Promoting Effects of Quercetin on Peripheral Nerves in Rats
Authors: Wang Walter , Huang Chih-Yang , Tsai Fuu-Jen , Tsai Chin-Chuan , Yao Chun-Hsu , Chen Yueh-Sheng 
Published: March 2013

Objectives The present study evaluated in vitro and in vivo the effects of quercetin (QC), a major ingredient in various flavonoids, on peripheral nerve regeneration. Methods In the in vitro study, we found that QC at concentrations of 0.1, 1, and 10 μg/mL could significantly promote the survival and outgrowth of cultured Schwann cells as compared with the controls treated with culture medium only. In the in vivo study, we evaluated peripheral nerve regeneration across a 15-mm gap in the sciatic nerve of the rat, using a silicone rubber nerve chamber filled with the QC solution. In the control group, the chambers were filled with normal saline only. Results At the end of 8 weeks, morphometric data revealed that all 3 QC groups significantly increased the count and density of myelinated axons as compared with the controls. Electro-physiological measurements showed that the QC-treated group at 1 μg/mL had a significantly larger area of evoked muscle action potential (MAP) compared with the controls. In addition, the amplitude of the MAP in the QC-treated groups at 0.1 and 1 μg/mL was significantly larger than that in the controls. Conclusions All of these results indicate that QC treatment has nerve growth–promoting effects which may lead to a promising herbal medicine for the recovery of regenerating peripheral nerves.