bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of inflammation on connexin 43 in chronic Chagas disease cardiomyopathy
Authors: Barreto Breno Cardim , Neves Maria Vitória Gomes das , Cardoso Carine Machado Azevedo , Meira Cássio Santana , Daltro Pâmela Santana , Figueira Cláudio Pereira , Santos Girlaine Café , Silva Daniela Nascimento , Távora Fábio , Neto João David de Souza , Macambira Simone Garcia , Lampe Paul D. , Coutinho Keyla Cristiny da Silva , Kasai Brunswick Tais Hanae , Ribeiro dos Santos Ricardo , Campos de Carvalho Antônio Carlos , Soares Milena Botelho Pereira 
Published: July 2024

BackgroundCardiac arrhythmias are the main cause of sudden death due to Chronic Chagasic Cardiomyopathy (CCC). Here we investigated alterations in connexin 43 (Cx43) expression and phosphorylation in cardiomyocytes as well as associations with cardiac arrhythmias in CCC.MethodsC57Bl/6 mice infected with Trypanosoma cruzi underwent cardiac evaluations at 6 and 12 months after infection via treadmill testing and EKG. Histopathology, cytokine gene expression, and distribution of total Cx43 and its phosphorylated forms Cx43S368 and Cx43S325/328/330 were investigated. Human heart samples obtained from subjects with CCC were submitted to immunofluorescence analysis. In vitro simulation of a pro-inflammatory microenvironment (IL-1β, TNF, and IFN-γ) was performed in H9c2 cells and iPSC-derived cardiomyocytes to evaluate Cx43 distribution, action potential duration, and Lucifer Yellow dye transfer.ResultsMice chronically infected with T. cruzi exhibited impaired cardiac function associated with increased inflammation, fibrosis and upregulated IL-1β, TNF, and IFN-γ gene expression. Confocal microscopy revealed altered total Cx43, Cx43S368 and Cx43S325/328/330 localization and phosphorylation patterns in CCC, with dispersed staining outside the intercalated disc areas, i.e., in lateral membranes and the cytoplasm. Reduced co-localization of total Cx43 and N-cadherin was observed in the intercalated discs of CCC mouse hearts compared to controls. Similar results were obtained in human CCC heart samples, which showed Cx43 distribution outside the intercalated discs. Stimulation of human iPSC-derived cardiomyocytes or H9c2 cells with IL-1β, TNF, and IFN-γ induced alterations in Cx43 localization, reduced action potential duration and dye transfer between adjacent cells.ConclusionHeart inflammation in CCC affects the distribution and phosphorylation pattern of Cx43, which may contribute to the generation of conduction disturbances in Chagas disease.
Triangular fossa of the third cerebral ventricle – an original 3D model and morphometric study
Authors: Nedelcu Alin Horatiu , Lupu Vasile Valeriu , Lupu Ancuta , Tepordei Razvan Tudor , Ioniuc Ileana , Stan Cristinel Ionel , Vicoleanu Simona Alice Partene , Haliciu Ana Maria , Statescu Gabriel , Ursaru Manuela , Danielescu Ciprian , Tarniceriu Cristina Claudia 
Published: July 2024

IntroductionThe triangular recess (TR), also called triangular fossa or vulva cerebri, represents the anterior extension of the diencephalic ventricle, located between the anterior columns of the fornix and the anterior white commissure. Over time, this structure of the third cerebral ventricle generated many disputes. While some anatomists support its presence, others have opposite opinions, considering that it only becomes visible under certain conditions. The aim of the study is to demonstrate the tangible structure of the triangular recess. Secondly, the quantitative analysis allowed us to establish an anatomical morphometric standard, as well as the deviations from the standard.Materials and methodsOur study is both a quantitative and a qualitative evaluation of the triangular fossa. We dissected 100 non-neurological adult brains, which were fixed in 10% formaldehyde solution for 10 weeks. The samples are part of the collection of the Institute of Anatomy, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi. We highlighted the triangular fossa by performing dissections in two stages at the level of the roof of the III ventricle.ResultsThe qualitative analysis is a re-evaluation of the classical data concerning the anatomy of the fossa triangularis. We proposed an original 3D model of the triangular recess in which we described a superficial part called vestibule and a deep part called pars profunda. We measured the sides of the communication between the two proposed segments, as well as the communication with the III ventricle. By applying the Heron’s formula, we calculated the area of the two communications. Statistical evaluations have shown that these communications are higher than they are wide. In addition, there is a statistical difference between the surfaces of the two communications: 34.07 mm2 ± 7.01 vs. 271.43 mm2 ± 46.36 (p = 0.001).ConclusionThe outcome of our study is both qualitative and quantitative. Firstly, we demonstrated the existence of the triangular fossa and we conceived a spatial division of this structure. Secondly, the measurements carried out establish an anatomo-morphometric norm of the triangular recess, which is useful in assessing the degree of hydrocephalus during the third endoscopic ventriculoscopy.
Machine learning-based gait adaptation dysfunction identification using CMill-based gait data
Authors: Yang Hang , Liao Zhenyi , Zou Hailei , Li Kuncheng , Zhou Ye , Gao Zhenzhen , Mao Yajun , Song Caiping 
Published: July 2024

BackgroundCombining machine learning (ML) with gait analysis is widely applicable for diagnosing abnormal gait patterns.ObjectiveTo analyze gait adaptability characteristics in stroke patients, develop ML models to identify individuals with GAD, and select optimal diagnostic models and key classification features.MethodsThis study was investigated with 30 stroke patients (mean age 42.69 years, 60% male) and 50 healthy adults (mean age 41.34 years, 58% male). Gait adaptability was assessed using a CMill treadmill on gait adaptation tasks: target stepping, slalom walking, obstacle avoidance, and speed adaptation. The preliminary analysis of variables in both groups was conducted using t-tests and Pearson correlation. Features were extracted from demographics, gait kinematics, and gait adaptability datasets. ML models based on Support Vector Machine, Decision Tree, Multi-layer Perceptron, K-Nearest Neighbors, and AdaCost algorithm were trained to classify individuals with and without GAD. Model performance was evaluated using accuracy (ACC), sensitivity (SEN), F1-score and the area under the receiver operating characteristic (ROC) curve (AUC).ResultsThe stroke group showed a significantly decreased gait speed (p = 0.000) and step length (SL) (p = 0.000), while the asymmetry of SL (p = 0.000) and ST (p = 0.000) was higher compared to the healthy group. The gait adaptation tasks significantly decreased in slalom walking (p = 0.000), obstacle avoidance (p = 0.000), and speed adaptation (p = 0.000). Gait speed (p = 0.000) and obstacle avoidance (p = 0.000) were significantly correlated with global F-A score in stroke patients. The AdaCost demonstrated better classification performance with an ACC of 0.85, SEN of 0.80, F1-score of 0.77, and ROC-AUC of 0.75. Obstacle avoidance and gait speed were identified as critical features in this model.ConclusionStroke patients walk slower with shorter SL and more asymmetry of SL and ST. Their gait adaptability was decreased, particularly in obstacle avoidance and speed adaptation. The faster gait speed and better obstacle avoidance were correlated with better functional mobility. The AdaCost identifies individuals with GAD and facilitates clinical decision-making. This advances the future development of user-friendly interfaces and computer-aided diagnosis systems.
Establishment of the Norwegian hearing register for children
Authors: Mattsson Tone Stokkereit , Nilsen Ann Helen , Wennberg Siri 
Published: July 2024

IntroductionThe Norwegian Directorate of Health approved the Norwegian Hearing Register for Children in 2022. The main objective of the register is to improve the quality of treatment for children with permanent hearing loss, by measures, follow-ups and monitoring the quality and results of the health care system.MethodsInclusion criteria are children who do not pass universal newborn hearing screening and/or children with permanent hearing loss &lt;18 years of age. Hearing loss is defined as pure-tone audiometry threshold of (PTA4) &gt; 20 dB in at least one ear. Data are registered at the Ear, Nose and Throat departments at inclusion and at follow-ups at the age of 3, 6, 10, and 15 years. The register collects information about the child within a holistic perspective. The key elements of the register are (a) data concerning newborn hearing screening; (b) data concerning hearing, medical information, hearing amplification and intervention (c) patient reported outcome measures registered by caregivers using three questionnaires; Pediatric Quality of Life Inventory, Strengths and Difficulties Questionnaire and Parents’ Evaluation of Aural/Oral Performance of Children.ResultsThe register has established four quality indicators regarding newborn hearing screening and early intervention (a) the rate of false positive neonatal screens; (b) testing for congenital cytomegalovirus within 3 weeks of age for children who do not pass newborn hearing screening; (c) audiological evaluation to confirm the hearing status no later than 3 months of age and (d) initiated intervention within 3 months after confirmation of hearing status.DiscussionThe register will include the total population of hearing impaired children over long time periods. Thus, the register enables each hospital to monitor their quality indicator scores continuously and compare them with national levels in real time. This facilitates and accelerates identification of improvement areas in the hospitals and will be an important contributor for quality improvement in NHS, diagnostics and hearing intervention for children in Norway. In addition, data from the register will be a unique source for research, and study designs with a long follow-up time can be applied.
Maternal vitamin D status during pregnancy and infant’s gut microbiota: a prospective cohort study
Authors: Song Qiying , Li Yao , Zhou Tao , Xiao Meiqun , Xiao Bin , Wang Mengying , Zhu Yuanfang 
Published: July 2024

ObjectivesTo prospectively explore the association of maternal serum 25(OH)D levels with the infant’s gut microbiota in Chinese populations, and to evaluate its potential influence on the dynamic change patterns of offspring’s gut microbiota from 1 to 6 months old.MethodsEighty-seven mother-infant dyads (vitamin D insufficient group vs. normal group = 59 vs. 28) were included in this longitudinal study. Two fecal samples were collected for the included infant at home by the parents at 1 month of age (“M1 phase”) and 6 months of age (“M6 phase”). Gut microbiota were profiled by 16S rRNA gene sequencing. We performed mixed effects models on alpha diversity metrics, PERMANOVA tests on beta diversity distances, and linear discriminant analysis (LDA) to identify differently abundant taxa.ResultsWe observed significantly lower Pielou’s evenness and Shannon diversity in the vitamin D insufficient group in the M6 phase (p = 0.049 and 0.015, respectively), but not in the M1 phase (p &gt; 0.05), and the dynamic changes in alpha diversity from 1 to 6 months old were significantly different according to maternal vitamin D status (p &lt; 0.05). There were also significant differences in gut microbiota composition between the vitamin D insufficient group and normal group, both in the M1 and M6 phases (LDA score &gt; 2.0, p &lt; 0.05). Moreover, among the predicted metagenome functions, pathways related to amino acid biosynthesis, starch degradation, and purine nucleotides biosynthesis were enriched in the vitamin D insufficient group.ConclusionOur findings highlight that maternal vitamin D status plays a pivotal role in shaping the early-life gut microbiota of the next generation.
A comparison of raspberry freezing-damage during preservation in isochoric and isobaric conditions
Authors: Câmpean Ștefan Ioan , Beșchea George Andrei , Vuțoiu Beatrice Georgiana , Tăbăcaru Maria Bianca , Năstase Gabriel 
Published: July 2024

IntroductionFruits are perishable, thus it’s crucial to have an efficient preservation technique that can increase storage time while keeping physical quality and nutritional attributes in order to avoid wastage. The majority of methods for long-term storage require refrigeration.MethodsIn this investigation, we assess the viability of isochoric freezing as a different technique of raspberry (Rubus idaeus L.) preservation. Raspberries were subjected to different storage conditions: isochoric freezing at –4°C, conventional isobaric settings at +3°C (refrigerator), –21°C (freezer), and –4°C with a trehalose solution in a plastic bag. The study assessed changes in weight loss, visual appearance, color, hardness, °Brix values, and pH over a seven-day period.ResultsKey findings reveal that raspberries subjected to isochoric freezing below the freezing point of water experienced minimal weight loss after seven days. Visual appearance, color, hardness, °Brix values, and pH were comparable to fresh raspberries, indicating minimal alteration.DiscussionThese results suggest that isochoric freezing shows potential as a preservation method that maintains the physical and chemical properties of raspberries similar to fresh fruit. Implementing diverse preservation techniques tailored to raspberries may contribute to environmental sustainability by reducing food wastage and the associated environmental impact.
Gasdermin D could be lost in the brain parenchyma infarct core and a pyroptosis-autophagy inhibition effect of Jie-Du-Huo-Xue decoction after stroke
Authors: Zhou Chang , Qiu Shi-wei , Wang Feng-ming , Liu Yu-chen , Hu Wei , Yang Mei-lan , Liu Wang-hua , Li Hua 
Published: July 2024

BackgroundThe Chinese ethnic medicine Jie-Du-Huo-Xue Decoction (JDHXD) is used to alleviate neuroinflammation in cerebral ischemia (CI). Our previous studies have confirmed that JDHXD can inhibit microglial pyroptosis in CI. However, the pharmacological mechanism of JDHXD in alleviating neuroinflammation and pyroptosis needs to be further elucidated. New research points out that there is an interaction between autophagy and inflammasome NLRP3, and autophagy can help clear NLRP3. The NLRP3 is a key initiator of pyroptosis and autophagy. The effect of JDHXD promoting autophagy to clear NLRP3 to inhibit pyroptosis on cerebral ischemia-reperfusion inflammatory injury is currently unknown. We speculate that JDHXD can inhibit pyroptosis in CI by promoting autophagy to clear NLRP3.MethodsChemical characterization of JDHXD was performed using LC-MS. Model of middle cerebral artery occlusion/reperfusion (MCAO/R) was established in SD rats. Neurological deficits, neuron damage, and cerebral infarct volume were evaluated. Western Blot and immunofluorescence were used to detect neuronal pyroptosis and autophagy.Results30 possible substance metabolites in JDHXD medicated serum were analyzed by LC-MS (Composite Score &gt; 0.98). Furthermore, JDHXD protects rat neurological function and cerebral infarct size after CI. JDHXD inhibited the expression of pyroptosis and autophagy after CI. Our western blot and immunofluorescence results showed that JDHXD treatment can reduce the expression of autophagy-related factors ULK1, beclin1, and LC3-Ⅱ. The expression of NLRP3 protein was lower in the JDHXD group than in the I/R group. Compared with the I/R group, the expressions of pyroptosis-related factors caspase-1 P 10, GSDMD-NT, IL-18, and IL-1β decreased in the JDHXD group. Furthermore, we observed an unexpected result: immunofluorescence demonstrated that Gasdermin D (GSDMD) was significantly absent in the infarct core, and highly expressed in the peri-infarct and contralateral cerebral hemispheres. This finding challenges the prevailing view that GSDMD is elevated in the ischemic cerebral hemisphere.ConclusionJDHXD inhibited pyroptosis and autophagy after MCAO/R. JDHXD suppressed pyroptosis and autophagy by inhibiting NLRP3, thereby alleviating CI. In addition, we present a different observation from previous studies that the expression of GSDMD in the infarct core was lower than that in the peri-infarct and contralateral non-ischemic hemispheres on day 3 of CI.
Underwater temperature and pressure monitoring for deep-sea SCUBA divers using optical techniques
Authors: Mirza Jawad , Kanwal Firdos , Salaria Umair Ahmad , Ghafoor Salman , Aziz Imran , Atieh Ahmad , Almogren Ahmad , Haq Anwar Ul , Kanwal Benish 
Published: July 2024

The safety of SCUBA divers remains at high risk in deep-sea owing to multiple factors such as dangerous surrounding, rely upon technical equipment necessary for life support, decreased underwater navigation, and communication infrastructure. Gradual decrease and increase in water temperature and pressure corresponding to depth are among the most common problems that cause most of the fatalities in deep-sea diving. Therefore, different gadgets for accurate measurement of vital parameters, reliable navigation, and seamless communication are of prime importance. In this paper, we propose an all-optical technique for local and remote monitoring of underwater temperature and pressure for deep-sea SCUBA divers based on fiber Bragg grating (FBG) sensors and underwater optical communication-single mode fiber (UWOC-SMF) integrated transmission system. The proposed technique is implemented using two FBG temperature and pressure sensors fixed over diver’s suit and UWOC-SMF integrated transmission system for simultaneous local and remote monitoring of underwater temperature and pressure. Remote monitoring of underwater temperature and pressure is achieved at ship station through a remotely operated underwater vehicle (ROV) and UWOC-SMF integrated transmission system by means of shifts in the original Bragg wavelengths of sensors due to temperature and pressure variations. The performance of the sensors is analyzed for pressure and temperature in the range of 0 to 6.4 MPa (≈0 to 655 mH2O) and 40 to −2°C, respectively corresponding to different depths. The results show that the proposed technique can work well in the deep ocean over a range of pressures and temperatures of 0–7 MPa and 40 to −2°C while achieving a temperature sensitivity of 4.3 p.m./°C and a pressure sensitivity of 30.5 p.m./MPa. Clear spectra of reflected signals from FBG sensors at ship station are achieved after signal transmission over UWOC-SMF hybrid link.
Three novel QTLs for FHB resistance identified and mapped in spring wheat PI672538 by bulked segregant analysis of the recombinant inbred line
Authors: Huang Qianglan , Li Xin , Li Qing , Zhong Shengfu , Li Xiuying , Yang Jiezhi , Tan Feiquan , Ren Tianheng , Li Zhi , Suizhuang Yang 
Published: July 2024

IntroductionFusarium head blight (FHB) has a large influence on both the yield and quality of wheat grain worldwide. Host resistance is the most effective method for controlling FHB, but unfortunately, very few genetic resources on FHB resistance are available; therefore, identifying novel resistance genes or quantitative trait loci (QTLs) is valuable. MethodsHere, a recombinant inbred line (RIL) population containing 451 lines derived from the cross L661/PI672538 was sown in four different environments (2019CZa, 2019CZb, 2021QL and 2021WJ).ResultsFive QTLs, consisting of two previously reported QTLs (FhbL693a and FhbL693b) and three new QTLs (FhbL693c, FhbL693d and FhbL693e), were identified. Further investigation revealed that FhbL693b, FhbL693c and FhbL693d could be detected in all four environments, and FhbL693a and FhbL693e were detected only in 2019CZb and 2021WJ, respectively. Among the QTLs, the greatest contribution (10.5%) to the phenotypic variation effect (PVE) was FhbL693d in 2021WJ, while the smallest (1.2%) was FhbL693e and FhbL693a in 2019CZb. The selection of 5Dindel-4 for FhbL693d, 4Aindel-7 for FhbL693c and 3Bindel-24 for FhbL693b decreased the number of damaged spikelets by 2.1, and a new line resistant to FHB named H140-2 was developed by marker-assisted selection (MAS). DiscussionThese results could help to further improve FHB resistance in the future.
Emotional responses of Korean and Chinese women to Hangul phonemes to the gender of an artificial intelligence voice
Authors: Lee Min-Sun , Lee Gi-Eun , Lee San Ho , Lee Jang-Han 
Published: July 2024

IntroductionThis study aimed to explore the arousal and valence that people experience in response to Hangul phonemes based on the gender of an AI speaker through comparison with Korean and Chinese cultures.MethodsTo achieve this, 42 Hangul phonemes were used, in a combination of three Korean vowels and 14 Korean consonants, to explore cultural differences in arousal, valence, and the six foundational emotions based on the gender of an AI speaker. A total 136 Korean and Chinese women were recruited and randomly assigned to one of two conditions based on voice gender (man or woman).Results and discussionThis study revealed significant differences in arousal levels between Korean and Chinese women when exposed to male voices. Specifically, Chinese women exhibited clear differences in emotional perceptions of male and female voices in response to voiced consonants. These results confirm that arousal and valence may differ with articulation types and vowels due to cultural differences and that voice gender can affect perceived emotions. This principle can be used as evidence for sound symbolism and has practical implications for voice gender and branding in AI applications.
Biocontrol Strategy of Listeria monocytogenes in Ready-to-Eat Pork Cooked Ham Using Peptic Hydrolysates of Porcine Hemoglobin
Authors: Sanchez-Reinoso Zain , Todeschini Sarah , Thibodeau Jacinthe , Ben Said Laila , Fliss Ismail , Bazinet Laurent , Mikhaylin Sergey 
Published: July 2024

Listeria monocytogenes is a foodborne pathogen that represents a serious concern for ready-to-eat (RTE) meat products due to its persistence in production facilities. Among the different strategies for the control of this pathogen, the use of antimicrobial peptides derived from food by-products, such as slaughterhouse blood proteins, has emerged as a promising biocontrol strategy. This study evaluated for the first time the use of peptic hydrolysates of porcine hemoglobin as a biocontrol strategy of L. monocytogenes in RTE pork cooked ham. Pure porcine hemoglobin (Hb-P) and porcine cruor (P-Cru) were hydrolyzed using pepsin at different temperatures (37 °C for Hb-P and 23 °C for P-Cru) for 3 h. Then, the hydrolysates were characterized in terms of their degree of hydrolysis (DH), peptide population, color, and antimicrobial activity (in vitro and in situ) against three different serotypes of L. monocytogenes. Reducing the hydrolysis temperature of P-Cru by 14 °C resulted in a 2 percentage unit decrease in DH and some differences in the peptide composition. Nevertheless, the antimicrobial activity (in situ) was not significantly impacted, decreasing the viable count of L. monocytogenes by ~1-log and retarding their growth for 21 days at 4 °C. Although the color of the product was visibly altered, leading to more saturated reddish and yellowish tones and reduced brightness, the discoloration of the hydrolysates can be addressed. This biopreservation approach holds promise for other meat products and contributes to the circular economy concept of the meat industry by valorizing slaughterhouse blood and producing new antilisterial compounds.