bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induksi dan Regenerasi Embrio Somatik Empat Jenis Ubi Kayu Menggunakan Beberapa Konsentrasi Pikloram
Authors: Rahman Nur Hamidar , Azizi Alfia Annur Aini , Hartati Sri , Saribanon Nonon 
Published: February 2025

Ubi kayu (Manihot esculenta Crantz) merupakan tanaman pokok penting di negara tropis. Di Indonesia, komoditas ini merupakan bahan pangan yang sangat popular dan banyak digunakan sebagai bahan baku untuk berbagai produk pangan olahan dan industri. Produksi kalus embriogenik yang dapat beregenerasi akan berguna dalam program pemuliaan bioteknologi untuk perbanyakan tanaman. Frekuensi tinggi pada pembentukan kalus embriogenik merupakan langkah kunci dalam sistem regenerasi ubi kayu khususnya untuk mendukung program pemuliaan modern. Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi pikloram terhadap pembentukan kalus dan regenerasi embrio somatik pada empat jenis ubi kayu. Leaf lobe yang berasal dari kultur in vitro digunakan sebagai eksplan untuk menginduksi kalus embriogenik pada media dasar MS dengan empat tingkat konsentrasi pikloram yaitu 3 mg/l, 5 mg/l, 10 mg/l, dan 12 mg/l. Evaluasi efisiensi produksi embrio somatik dilakukan berdasarkan persentase kalus embriogenik yang terbentuk dalam setiap perlakuan. Kalus embrio somatik selanjutnya dipindahkan ke media maturasi MSN ( 1 mg/ml MS+NAA +1 mg/l CuSO4.) Setelah tumbuh kotiledon pada media MSN, dipindahkan ke media MS yang mengandung 1 mg/l BAP. Pendewasaan tunas dilakukan pada media MS tanpa ZPT selama 5 -7 minggu. Aklimatisasi dilakukan pada media tanam yang terdiri dari tanah:kompos (1:1). Hasil penelitian menunjukkan bahwa perlakuan dengan media MS yang mengandung pikloram pada konsentrasi 3 mg/l, 5 mg/l, 10 mg/l, dan 12 mg/l dapat menginduksi pembentukan kalus dari eksplan leaf lobe ubi kayu Adira 4, Carvita 25, Manggu dan Menti menghasilkan persentase eksplan berkalus 100 %, sedangkan pada media kontrol (tanpa pikloram) tidak menghasilkan kalus. Pada semua konsentrasi pikloram yang diuji, keempat jenis ubi kayu dapat membentuk kalus embriogenik. Persentase pembentukan kalus embriogenik tertinggi yaitu sebesar 83,3% diperoleh pada media MS yang mengandung 5 mg/l pikloram untuk Carvita 25 dan Menti. Waktu tercepat untuk pembentukan kalus embriogenik adalah 12 hari dan paling lama adalah 51 hari. Formulasi media untuk regenerasi ubi kayu melalui jalur embrio somatik yang diperoleh dari penelitian ini akan bermanfaat untuk produksi bibit ubi kayu dan juga bisa digunakan untuk transformasi genetik untuk perbaikan kualitas ubi kayu.
Optimalisasi Limbah Sorgum Lokal Sumba Sebagai Pupuk Organik dan Pengaruhnya Terhadap Sifat Kimia Tanah
Authors: Jawang Uska Peku , Mbana Febyningsi Rambu Ladu , Yewa Darius Meta , Windi Arman Kambaru , Mbita Ones Pajaru , Liau Frans Meta Pangga 
Published: February 2025

Masalah yang muncul saat musim tanam di tingkat petani adalah terbatasnya ketersediaan pupuk, maka pemenuhan kebutuhan pupuk wajib dilakukan. Langkah konkret yang diambil yaitu dengan memanfaatkan potensi bahan organik lokal, guna membuat pupuk. Sorgum merupakan jenis serealia yang memiliki potensi yang dapat dikembangkan melalui limbah hasil produksi. Sumba Timur memiliki keanekaragaman sorgum yang dapat memberikan potensi bahan organik untuk bahan dalam pembuatan pupuk organik yang berkualitas. Penelitian ini merupakan penelitian eksperimen dengan dua tahapan yaitu mengomposkan limbah empat jenis sorgum lokal dan 2) pemberian perlakuan kompos limbah sorgum ke tanah. Kelima jenis kompos dianalisis untuk mengetahui kualitas pupuk berdasarkan SNI 19-7030-2004. Pupuk hasil kompos dari beberapa limbah sorgum di aplikasikan ke tanah untuk melihat pengaruh terhadap sifat kimia tanah dengan menggunakan rancangan acak lengkap. Rancangan perlakuan yaitu 6 perlakuan dan diulang sebanyak 4 kali. Jenis perlakuan yaitu S0: kontrol, S1: kompos Jarada Anahida, S2: kompos Jarabi Anahida, S3: kompos Jaraga Anahida, S4: kompos Jarala Anahida, dan S5: Jarahammu Anahida. Pada uji kualitas pupuk parameter ujinya adalah temperatur, warna, aroma, pH, kadar air, bahan organik, nitrogen, fosfor, kalium dan C/N rasio. Uji pengaruh kompos pada sifat kimia tanah parameter yang diuji yaitu pH, c-organik, nitrogen, fosfor, kalium dan kapasitas tukar kation. Berdasarkan hasil pengujian terhadap kelima jenis pupuk kompos lokal berbahan dasar limbah sorgum yang memenuhi standar SNI 19-7030-2004 yaitu kompos Jarabi Anahida. Keempat jenis kompos Jarada Anahida, Jaraga Anahida, Jarala Anahida, dan Jarahammu Anahida belum memenuhi SNI. Parameter uji yang mempengaruhi ketidaksesuaian dengan SNI pupuk kompos yaitu warna, pH dan bahan organik. Kompos berpengaruh terhadap sifat kimia tanah pada pH terjadinya penurunan pH sedangkan pada C-organik, N-total, P, K dan KTK mengalami peningkatan. Kompos yang berpengaruh terhadap sifat kimia tanah dengan nilai tertinggi yaitu pemberian kompos Jarahammu Anahida (S5). Rekomendasi perbaikan untuk dapat meningkatkan kualitas pupuk kompos yaitu dengan memperhatikan bahan organik yang digunakan, media dan lama mengomposkan.
Las personas sin hogar: una población ante los ojos de enfermería
Authors: Alonzo-Zamora María Fernanda , Ruiz-Vallejo Abigail de Jesús , Altamira-Camacho Ramiro 
Published: February 2025

Introducción. Las personas sin hogar, a menudo se ven envueltas en las inequidades e injusticias sociales, donde en su mayoría se les reconoce como un humano y no como una persona jurídica constituida conforme a la ley. Desarrollo. Cuando las personas sin hogar son excluidas existe una destrucción del “hogar” donde viven su espacialidad, donde desarrollan sus costumbres, su religión y su espiritualidad; por lo que, cualquier abuso a su persona, corporalidad, historicidad y espacialidad, es una despersonalización a su ser y a su cultura. Es necesario tener en cuenta las repercusiones y obstáculos que dificultan el desarrollo de los derechos humanos, en específico el derecho a la salud como campo de desarrollo de la enfermería. ya que se pueden crear líneas de acción para la salud o gestión de cuidados de enfermería que contribuyan a darle voz a las personas sin hogar y sus necesidades tan importantes. Conclusión. Es necesario el reconocimiento de estas situaciones para que enfermería, pueda visibilizar o voltear a “mirar” y como una profesión que puede dar voz a las necesidades de esta población, a los vulnerables, crear líneas de acción para su cuidado ya que responde a las necesidades de salud de las personas en todos los entornos y a lo largo de todo el ciclo vital, dándole un lugar a sus derechos, donde en especial las personas sin hogar son susceptibles a exclusión, desigualdad y estigmatización social. El propósito de este ensayo es dar a conocer el fenómeno del cuidado en las personas sin hogar, vinculado a la justicia social y a los nuevos paradigmas del cuidado desde las experiencias interpersonales, dando lugar al conocimiento de un fenómeno poco abordado por Enfermería.
Patrones de conocimiento de Carper y cuidados de enfermería en paciente con urolitiasis y obesidad
Authors: Chan Santiago Juan Manuel , García Campos María de Lourdes , Patiño López María Esther 
Published: February 2025

Introducción. Según Orem, cuando existe un déficit de autocuidado la agencia de enfermería debe desarrollar parcial o totalmente las actividades de autocuidado para lograr un estado óptimo salud, para ello se debe implementar una estructura sintáctica del conocimiento como los Patrones de Conocimiento de Carper (PCC). Objetivo. Evaluar los cuidados de enfermería de una paciente con desviación del estado de salud a causa de urolitiasis y obesidad con base en el proceso de enfermería, los PCC y la Teoría General del Déficit de Autocuidado (TGDAC). Método. Se utilizó la TGDAC, los PCC (empírico, estético y ético) y las taxonomías de la NANDA, NOC y NIC. Valoración. Femenina de 46 años, que acudió a urgencias por cólico renal y dolor lumbar; antecedentes de infección de vías urinarias y obesidad. Se valoraron sus factores condicionantes básicos de desarrollo, salud, modelo de vida, sistema sanitario, familia, factores socioculturales, recursos, entorno, y sus requisitos de autocuidado universales y del desarrollo. Diagnósticos. Diagnósticos de enfermería relacionado con el dolor, eliminación urinaria y obesidad. Planeación y ejecución. Las intervenciones se enfocaron en el manejo del dolor agudo, cuidados del catéter urinario, asesoramiento nutricional, alimentación y ayuda para disminuir el peso. Evaluación. Aumentaron las puntuaciones postintervención de los indicadores del dolor (8 a 21), eliminación urinaria (7 a 12) y estado nutricional (8 a 15). Conclusión. Con el sistema de enfermería total y parcialmente compensatorio, la paciente consiguió mejoras en la desviación del estado de salud; sin embargo, debe incorporar a su vida diaria actividades de autocuidado.
Direitos Humanos, Reflexões digitais e Tributação Justa
Authors: Macena de Araújo Jailton , Katarina de Medeiros Costa Hirdan 
Published: February 2025

Os Direitos Humanos são princípios universais que garantem a dignidade, liberdade, igualdade e justiça para todas as pessoas, independentemente de sua origem, raça, sexo, cor, idade ou quais outra característica cultural ou condição. Esses direitos abrangem dimensões amplas e interdependentes, como os direitos civis, políticos, econômicos, sociais e culturais. Entre os exemplos mais relevantes, destacam-se o direito à vida, à liberdade de expressão, ao trabalho digno, à educação de qualidade e à saúde universal e acessível. Fundamentados em tratados e convenções internacionais e protegidos pelas legislações nacionais, os direitos humanos são pilares universais para a promoção da paz, da justiça e do bem-estar social.

Paralelamente, vivemos em um contexto de transformação digital, em que as tecnologias digitais estão integradas a praticamente todos os aspectos da vida em sociedade. Essa revolução tecnológica redefine a forma como indivíduos, empresas e governos operam e interagem, trazendo desafios e oportunidades. Por um lado, a digitalização pode ampliar o acesso a direitos básicos, como educação e saúde, e promover maior transparência nas relações institucionais, ampliando assim a gama e o acesso aos direitos humanos. Por outro, exige um debate aprofundado sobre questões como privacidade,

exclusão digital e a necessidade de regulação ética para evitar desigualdades tecnológicas. Outro elemento central para a construção de uma sociedade justa é a tributação. Uma política tributária justa não apenas garante a redistribuição de riqueza, como também desempenha um papel essencial no estímulo ao desenvolvimento socioeconômico e na manutenção da infraestrutura e dos serviços essenciais como saúde, educação e segurança. Ao promover uma alocação equilibrada de recursos, a tributação contribui diretamente para a redução de desigualdades sociais e para a construção de uma economia sustentável e inclusiva.

Assim, a articulação entre Direitos Humanos, transformações digitais e políticas de tributação revela-se crucial para o enfrentamento dos desafios do mundo contemporâneo. Compreender as intersecções entre esses pilares é essencial para traçar caminhos que assegurem desenvolvimento social e econômico sem comprometer os valores fundamentais da dignidade humana.
Kegiatan sosial kemasyarakatan oleh Yayasan Buddha Tzu Chi di Perumahan Cinta Kasih Lubuk Buaya
Authors: Julita Rahma , Montessori Maria , Ananda Azwar , Isnarmi Isnarmi 
Published: February 2025

Tujuan penelitian ini adalah untuk mengetahui bentuk-bentuk kegiatan sosial kemasyarakatan yang dilakukan oleh Yayasan Buddha Tzu Chi di Perumahan Cinta Kasih Lubuk Buaya Kota Padang, faktor yang melatarbelakangi kegiatan sosial dilakukan, dan Hambatan yang dihadapi Yayasan Buddha Tzu Chi dalam mengelola kegiatan sosial di Perumahan Cinta Kasih. Penelitian ini menggunakan pendekatan kualitatif dengan metode deskriptif. Teknik penentuan objek dalam penelitian ini dengan teknik purposive sampling. Prosedur pengumpulan data yang digunakan adalah observasi, wawancara, dan dokumentasi. Teknik analisis data yaitu melalui cara reduksi data, penyajian data, dan pengambilan kesimpulan. Hasil penelitian ini menunjukkan bahwa bentuk-bentuk kegiatan sosial yang dilakukan di Perumahan Cinta Kasih adalah program celengan kasih, program daur ulang, dan program pembagian sembako dan hampers ketika hari besar. Faktor yang melatarbelakangi kegiatan sosial di Perumahan Cinta Kasih diantaranya permintaan Pemerintah Kota Padang kepada Yayasan Buddha Tzu Chi untuk melanjutkan pendirian Perumahan Cinta Kasih, adanya kesepakatan antara warga Perumahan Cinta Kasih dengan Yayasan sehingga kegiatan sudah biasa dilakukan. Hambatan yang dihadapi Yayasan Buddha Tzu Chi dalam melaksanakan kegiatan sosial di Perumahan Cinta Kasih diantaranya adalah rendahnya partisipasi warga dalam melaksanakan kegiatan sosial yang dilakukan, kurangnya kesadaran warga dalam menjaga kebersihan Lingkungan, dan rendahnya tingkat perekonomian masyarakat.
EXPERIMENTAL VALIDATION OF THE WAY FOR EVALUATING THE SPREAD OF ACUTE PERITONITIS
Authors: Grynchuk F. F. , Grynchuk F. V. 
Published: February 2025

Abstract Objective. To explore way for objectively assessing the spread of acute peritonitis in experimental settings. Materials and methods. A study was conducted on 50 albino non-pedigree rats, divided into two groups: 10 served as the control group, and 40 were part of the research group. Acute peritonitis was induced in the research group through an intraperitoneal injection of a 20 % autofecal mixture. Both groups underwent laparotomy at intervals of 6, 12, 24, and 48 hours following the induction of peritonitis. During these procedures, the width of the scattering zone of laser beams with wavelengths of 0.63 μm and 0.4 μm was measured on the parietal peritoneum. Subsequently, the peritoneum was collected for histological examination. Results. In the initial stages, no changes were observed in the histological structure of the peritoneum and adjacent tissues. However, serous inflammation of the peritoneum was detected 6 hours after modeling, which later progressed to purulent inflammation. At the same time, the scattering zone of a laser beam with a wavelength of λ=0.63 μm increased significantly and continued to do so consistently. A similar pattern was observed for a beam with a wavelength of λ=0.4 μm. By 48 hours, the measurements were similar to those at 24 hours. The ratio of the scattering zone widths for the two wavelengths increased significantly 6 hours after simulating peritonitis and remained stable thereafter. Conclusions. 1. The scattering zone width ratio of laser beams with wavelengths of λ=0.63 μm and λ=0.5 μm shows a statistically significant difference between intact and inflamed areas of the rats&#39; parietal peritoneum. 2. In the intact peritoneum, the ratio indicators are less than 2 units, whereas in the inflamed peritoneum, these parameters increase significantly.
MORPHOLOGICAL CHANGES OF THE COSTAL PART OF THE DIAPHRAGM ARE CAUSED BY PNEUMOPERITONEUM OF MODERATE PRESSURE IN THE EXPERIMENT
Authors: Kritsak M. 
Published: February 2025

Laparoscopy, one of the minimally invasive procedures, is a widely used procedure for the diagnosis and treatment of various types of clinical problems. Pneumoperitoneum during laparoscopy causes a displacement of the diaphragm by 1-3 cm. towards the pleural cavity and can cause problems in organs and tissues after surgery. Does the morphological structure of the diaphragm change?
Aim. To investigate the histological structure of the costal part of the diaphragm after intra-abdominal pressure created by carbon dioxide for different times.
Material and methods. The experimental study was performed on 40 animals, which were divided into 4 groups of 10 rats each as follows. Group I - an intact group, which was not modeled with any pathology, was used as an indicator of the norm of the morphological picture. Group II – created PP for 1 hour, Group III – created PP for 3 hours and Group IV – created PP for 5 hours. After which the required part of the diaphragm was selected.
Results. The obtained data on the morphology of the intact group indicated the signs characteristic of this group of muscles. In Group II, muscle fibers with clear transverse striation were detected, located compactly, variability in thickness was observed, and they took a wavy arrangement. In Group IIІ animals, transverse striation is visualized fragmentarily, the nuclei are located unevenly with dislocation to the center. Signs of venous congestion were observed. The connective tissue is loosened by edema, there are low-intensity cellular infiltrates in the composition of which lymphocytes and histiocytes predominated. When studying the materials of Group IV animals, a deepening of destructive changes was observed. Muscle fibers are compactly arranged, but the boundaries are not clear. Eosinophilic areas alternate with hypochromic, in places there is sarcoplasmic decay and myocytolysis. Venous and capillary engorgement, there is a distribution leukocytosis.
Conclusion. It was established that pneumoperitoneum at a level of 5 mm Hg. leads to the appearance of destructive changes in the costal part of the diaphragm and is comparable to the intact group. The severity of these changes depends on the duration of intra-abdominal pressure created in this experimental model.
FEATURES OF REMODELING OF STRUCTURAL COMPONENTS OF RAT KIDNEYS USING VARIOUS REHYDRATION METHODS AFTER SEVERE GENERAL DEHYDRATION
Authors: Herasymiuk I. , Vakhnovska Kh. 
Published: February 2025

When restoring water balance after dehydration, it is important to consider a number of factors, since the wrong correction can be harmful and have undesirable consequences.
The purpose of the study: to establish the features of the influence of various methods of rehydration after general dehydration on the structural reorganization of the kidneys of white rats. The experiments were conducted on white rats. General dehydration was simulated by feeding dry oats without access to water for 10 days. In 3 groups, water balance was restored with dosed drinking water consumption, unlimited water consumption and unlimited consumption of saline. The study was conducted after 1, 3, 6 and 10 days.
Morphometric studies of histological sections of the kidneys included determination of: the area of the glomerulus with the capsule, the area of the capillary loops of the glomerulus and the area of the urinary space. The use of different methods of rehydration after dehydration has a qualitatively identical, but quantitatively different effect on the recovery processes in the kidneys, which are based on different types of reactions of blood vessels. With unlimited consumption of drinking water, an increase in the tone of smooth muscle membranes with thickening of the walls of the arteries was observed after just one day. Moreover, if in the interlobular arteries this was a progression of the process that arose as a result of dehydration, then in the arcuate and interlobular arteries dehydration initially caused dilatation reactions, and they were replaced by reactions of the opposite direction. As a result, due to edema of the tubular epithelium surrounding the renal corpuscles, the latter decreased in size. The size of the capillary glomeruli also decreased. The urinary spaces continued to remain dilated. After 3 days of the recovery period, reversible changes were noted in the renal corpuscles and vessels, which continued in the next 6 and 10 days of observation. Similar, but quantitatively less pronounced were the changes with unlimited use of saline solution. The most effective qualitatively was the dosed use of water.
Thus, the use of different methods of restoring water balance after dehydration has a qualitatively identical, but quantitatively different effect on the structural reorganization of the kidneys. The most effective qualitatively and quantitatively was the dosed use of water.
CLINICAL EFFECTIVENESS OF PRP THERAPY IN THE COMPREHENSIVE TREATMENT OF TROPНICAL ULCERATIONS OF THE LOWER EXTREMITIES
Authors: Polyovyi V. , Pavlunishin R. , Plehutsa O. , Palyanitsa A. , Proskurniak M. , Fordzun O. , Volianiuk K. 
Published: February 2025

Treatment of trophic ulcers of the lower extremities of various origins remains one of the current scientific and practical problems of surgery today. In recent years, the use of autologous platelet-rich plasma as a factor for stimulating soft tissue regeneration has gained considerable popularity. The aim of this study was to evaluate the effectiveness of PRP therapy in the complex treatment of trophic ulcers of the lower extremities.
When analyzing the obtained data, a positive effect of the PRP therapy method on the early appearance of granulation tissue was revealed, and its smaller effect on the epithelialization process compared to the comparison group. In each specific case, the presence of concomitant vascular pathology and metabolic changes was taken into account, but the trend of the healing process indicated a clear positive effect of the PRP therapy method on wound cleansing and the appearance of granulations earlier in patients in the main group than in the comparison group. The dynamics had a linear, decreasing nature, which was clinically characterized by a decrease in inflammatory changes in the wound, already after the first 3 days of treatment using the specified method. In the comparative analysis, it should be noted that the largest changes, the difference in the indicators of patients in the main group occurred in the indicators &quot;Swelling&quot; and &quot;Exudation&quot;, a somewhat smaller dynamics of changes was observed in the indicator &quot;Hyperemia&quot;.
Conclusion. The use of the treatment method using autologous platelet-rich plasma allowed to reduce the patient&#39;s hospital stay by more than 10 %, accelerated the cleansing of the wound from necrotic tissues and the transition of the wound process to the second phase, and stimulated the early appearance of granulations several days earlier than in patients in the comparison group.