bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmentally Friendly Determination of Quality Parameters of Biodiesel/Diesel Blends Using Fourier Transform Infrared Spectra
Authors: Ruschel Carla Felippi Chiella , Huang Chun Te , Samios Dimitrios , Ferrão Marco Flôres , Yamamoto Carlos Itsuo , Plocharski Rubia Carla Barato 
Published: February 2015

AbstractMultivariate calibration models based on data from mid‐infrared spectroscopy of biodiesel/diesel blends were obtained. The blends were prepared from diesel oil and esters of soybean oil, waste cooking oil, and hydrogenated vegetable oil in proportions ranging from 0 to 100 % biodiesel. The results showed that the multivariate regression models with interval partial least squares (iPLS), backward interval partial least squares (biPLS), and synergy interval partial least squares (siPLS) were able to determine the fractions of the infrared spectrum that contain the relevant information for estimating the values of physicochemical properties, flash point, specific gravity, and cetane number, which are used in quality control of the blends. In the best models, the values of determination coefficients were greater than 0.9500, proving their efficiency as an alternative to traditional analytical methods.
Determination of Iron and Copper in Edible Oils by Flame Atomic Absorption Spectrometry After Liquid–Liquid Extraction
Authors: Tokay Feyzullah , Bağdat Sema 
Published: February 2015

AbstractIn the present work, a novel and rapid extraction method for Fe(III) and Cu(II) from liquid edible oils is described. N,N′‐Bis(4‐methoxysalicylidene)‐1,2‐diamino ethane (MSE) was utilized for the extraction of metal ions and the determination was achieved by flame atomic absorption spectrometry (FAAS). In order to optimize the extraction conditions, a central composite design (CCD) technique was employed. The optimum conditions, the ratio of MSE solution volume to oil mass, stirring time and temperature, were determined as 1.2 and 1.1 mL g−1; 24.0 and 31.8 min and 25.3 and 33.2 °C for Fe(III) and Cu(II) extraction, respectively. The feasibility of the improved method was tested with oil‐based metal standards affording 98.6 % recovery for both metals and 67.3 ng g−1 for Fe(III) and 15.3 ng g−1 for Cu(II) as limit of detection (LOD).
An All‐Natural Adhesive for Bonding Wood
Authors: Jang Yonghwan , Li Kaichang 
Published: February 2015

AbstractA novel adhesive that is solely based on natural materials of defatted soy flour (SF) and magnesium oxide (MgO) has been investigated for preparation of five‐ply plywood panels. The resulting plywood panels met the industrial water‐resistant requirement for interior plywood. In this study, mechanisms by which an aqueous mixture of SF and MgO served as a strong and water‐resistant adhesive for bonding wood were investigated. SF was first fractionated into soy protein isolates (SPI), a water‐soluble fraction, and insoluble carbohydrates (ICs) that were mixed with MgO, respectively, for preparation of maple laminates. The water resistance of the resulting maple laminates was evaluated by a three‐cycle water‐soaking‐and‐drying (WSAD) test and a two‐cycle boiling‐water test (BWT). The mixture of MgO and the soluble fraction was not able to bond maple veneers together. The shear strengths of the resulting maple laminates before and after WSAD and BWT all had the following order: MgO–SPI > MgO–SF > SF only > MgO–IC. The water solubility of SF in the heat‐cured SF–MgO mixture was much lower than that of the heat‐cured SF. We believe that the low water solubility of SF–MgO and close interactions between MgO and soy proteins instead of soy carbohydrates were responsible for the superior strengths and high water resistance of the soy‐MgO adhesive.
Incidence of eclampsia with HELLP syndrome and associated mortality in Latin America
Authors: Vigil‐De Gracia Paulino , Rojas‐Suarez José , Ramos Edwin , Reyes Osvaldo , Collantes Jorge , Quintero Arelys , Huertas Erasmo , Calle Andrés , Turcios Eduardo , Chon Vicente Y. 
Published: February 2015

AbstractObjectiveTo describe the maternal outcome among women with eclampsia with and without HELLP syndrome (hemolysis, elevated liver enzymes, and low platelet count).MethodsA cross‐sectional study of women with eclampsia was undertaken in 14 maternity units in Latin America between January 1 and December 31, 2012. Outcomes were compared between women with and without concomitant HELLP syndrome. Logistic regression analysis was performed to identify independent risk factors of maternal mortality.ResultsThere were 196 eclampsia cases among 115 038 deliveries; 142 (72.4%) women had eclampsia alone and 54 (27.6%) women had concomitant HELLP syndrome. Severe systolic hypertension (≥ 160 mm Hg), severe diastolic hypertension (≥ 110 mm Hg), and hypertensive encephalopathy were significantly more common among women with HELLP than among those with eclampsia alone (P = 0.01 for all). There were 8 (4.1%) maternal deaths, all in the group with HELLP syndrome, and 18 (9.1%) perinatal deaths. In a multivariate regression model, maternal mortality was significantly associated with low platelet count and severe systolic hypertension (P < 0.05).ConclusionEclampsia with HELLP syndrome is a dangerous complication associated with pregnancy. Low platelet count secondary to HELLP syndrome and severe systolic hypertension were independently associated with maternal mortality from eclampsia.
NxTrim: optimized trimming of Illumina mate pair reads
Authors: O’Connell Jared , Schulz-Trieglaff Ole , Carlson Emma , Hims Matthew M. , Gormley Niall A. , Cox Anthony J. 
Published: February 2015

Abstract
Motivation: Mate pair protocols add to the utility of paired-end sequencing by boosting the genomic distance spanned by each pair of reads, potentially allowing larger repeats to be bridged and resolved. The Illumina Nextera Mate Pair (NMP) protocol uses a circularization-based strategy that leaves behind 38-bp adapter sequences, which must be computationally removed from the data. While ‘adapter trimming’ is a well-studied area of bioinformatics, existing tools do not fully exploit the particular properties of NMP data and discard more data than is necessary.
Results: We present NxTrim, a tool that strives to discard as little sequence as possible from NMP reads. NxTrim makes full use of the sequence on both sides of the adapter site to build ‘virtual libraries’ of mate pairs, paired-end reads and single-ended reads. For bacterial data, we show that aggregating these datasets allows a single NMP library to yield an assembly whose quality compares favourably to that obtained from regular paired-end reads.
Availability and implementation: The source code is available at https://github.com/sequencing/NxTrim
Contact: acox@illumina.com
Supplementary information: Supplementary data are available at Bioinformatics online.
Mechanical Ventilation Induces Neutrophil Extracellular Trap Formation
Authors: Yildiz Christopher , Palaniyar Nades , Otulakowski Gail , Khan Meraj A. , Post Martin , Kuebler Wolfgang M. , Tanswell Keith , Belcastro Rosetta , Masood Azhar , Engelberts Doreen , Kavanagh Brian P. 
Published: February 2015

AbstractAbstract
By creating lung injury in mice using intratracheal lipopolysaccharide and mechanical ventilation, the authors documented that neutrophil extracellular traps were formed when lipopolysaccharide was present during high tidal ventilation and could be treated with deoxyribonuclease I. The treatment cleared the neutrophil extracellular traps and improved lung mechanics, but other measures of lung injury (including increased neutrophil count, increased protein content in lavage fluid, and low oxygenation) persisted, documenting a limited role for the neutrophil extracellular traps in this form of lung injury.
Supplemental Digital Content is available in the text.

Background:
Mechanical ventilation can injure the lung and induce a proinflammatory state; such ventilator-induced lung injury (VILI) is associated with neutrophil influx. Neutrophils release DNA and granular proteins as cytotoxic neutrophil extracellular traps (NETs). The authors hypothesized that NETs were produced in a VILI model and may contribute to injury.

Methods:
In a two-hit lipopolysaccharide/VILI mouse model with and without intratracheal deoxyribonuclease (DNase) treatment or blockade of known inducers of NET formation (NETosis), the authors assessed compliance, bronchoalveolar lavage fluid protein, markers of NETs (citrullinated histone-3 and DNA), and markers of inflammation.

Results:
Although lipopolysaccharide recruited neutrophils to airways, the addition of high tidal mechanical ventilation was required for significant induction of NETs markers (e.g., bronchoalveolar lavage fluid DNA: 0.4 ± 0.07 µg/ml [mean ± SEM], P < 0.05 vs. all others, n = 10 per group). High tidal volume mechanical ventilation increased airway high-mobility group box 1 protein (0.91 ± 0.138 vs. 0.60 ± 0.095) and interleukin-1β in lipopolysaccharide-treated mice (22.4 ± 0.87 vs. 17.0 ± 0.50 pg/ml, P < 0.001) and tended to increase monocyte chemoattractant protein-1 and interleukin-6. Intratracheal DNase treatment reduced NET markers (bronchoalveolar lavage fluid DNA: 0.23 ± 0.038 vs. 0.88 ± 0.135 µg/ml, P < 0.001; citrullinated histone-3: 443 ± 170 vs. 1,824 ± 403, P < 0.01, n = 8 to 10) and attenuated the loss of static compliance (0.9 ± 0.14 vs. 1.58 ± 0.17 ml/mmHg, P < 0.01, n = 19 to 20) without significantly impacting other measures of injury. Blockade of high-mobility group box 1 (with glycyrrhizin) or interleukin-1β (with anakinra) did not prevent NETosis or protect against injury.

Conclusions:
NETosis was induced in VILI, and DNase treatment eliminated NETs. In contrast to experimental transfusion-related acute lung injury, NETs do not play a major pathogenic role in the current model of VILI.
Formation and Dissociation of the BSS1 Protein Complex Regulates Plant Development via Brassinosteroid Signaling
Authors: Shimada Setsuko , Komatsu Tomoyuki , Yamagami Ayumi , Nakazawa Miki , Matsui Minami , Kawaide Hiroshi , Natsume Masahiro , Osada Hiroyuki , Asami Tadao , Nakano Takeshi 
Published: February 2015

Abstract
Brassinosteroids (BRs) play important roles in plant development and the response to environmental cues. BIL1/BZR1 is a master transcription factor in BR signaling, but the mechanisms that lead to the finely tuned targeting of BIL1/BZR1 by BRs are unknown. Here, we identified BRZ-SENSITIVE-SHORT HYPOCOTYL1 (BSS1) as a negative regulator of BR signaling in a chemical-biological analysis involving brassinazole (Brz), a specific BR biosynthesis inhibitor. The bss1-1D mutant, which overexpresses BSS1, exhibited a Brz-hypersensitive phenotype in hypocotyl elongation. BSS1 encodes a BTB-POZ domain protein with ankyrin repeats, known as BLADE ON PETIOLE1 (BOP1), which is an important regulator of leaf morphogenesis. The bss1-1D mutant exhibited an increased accumulation of phosphorylated BIL1/BZR1 and a negative regulation of BR-responsive genes. The number of fluorescent BSS1/BOP1-GFP puncta increased in response to Brz treatment, and the puncta were diffused by BR treatment in the root and hypocotyl. We show that BSS1/BOP1 directly interacts with BIL1/BZR1 or BES1. The large protein complex formed between BSS1/BOP1 and BIL1/BZR1 was only detected in the cytosol. The nuclear BIL1/BZR1 increased in the BSS1/BOP1-deficient background and decreased in the BSS1/BOP1-overexpressing background. Our study suggests that the BSS1/BOP1 protein complex inhibits the transport of BIL1/BZR1 to the nucleus from the cytosol and negatively regulates BR signaling.
Successful selection of an infection‐protective anti‐Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models
Authors: Ohsawa Hiroyoshi , Baba Tadashi , Enami Jumpei , Hiramatsu Keiichi 
Published: February 2015

ABSTRACTRecent clinical trials to develop anti‐methicillin‐resistant Staphylococcus aureus (MRSA) therapeutic antibodies have met unsuccessful sequels. To develop more effective antibodies against MRSA infection, a panel of mAbs against S. aureus cell wall was generated and then screened for the most protective mAb in mouse infection models. Twenty‐two anti‐S. aureus IgG mAbs were obtained from mice that had been immunized with alkali‐processed, deacetylated cell walls of S. aureus. One of these mAbs, ZBIA5H, exhibited life‐saving effects in mouse models of sepsis caused by community‐acquired MRSA strain MW2 and vancomycin‐resistant S. aureus strain VRS1. It also had a curative effect in a MW2‐caused pneumonia model. Curiously, the target of ZBIA5H was considered to be a conformational epitope of either the 1,4‐β‐linkage between N‐acetylmuramic acid and N‐acetyl‐D‐glucosamine or the peptidoglycan per se. Reactivity of ZBIA5H to S. aureus whole cells or purified peptidoglycan was weaker than that of most of the other mAbs generated in this study. However, the latter mAbs did not have the protective activities against S. aureus that ZBIA5H did. These data indicate that the epitopes that trigger production of high‐yield and/or high‐affinity antibodies may not be the most suitable epitopes for developing anti‐infective antibodies. ZBIA5H or its humanized form may find a future clinical application, and its target epitope may be used for the production of vaccines against S. aureus infection.
Replication of IMR‐32‐adapted JC virus clones in human embryonic kidney cells
Authors: Nukuzuma Souichi , Sugiura Shigeki , Nakamichi Kazuo , Kameoka Masanori , Nukuzuma Chiyoko , Tasaki Takafumi , Takegami Tsutomu 
Published: February 2015

ABSTRACTIt has been difficult to study JCV replication because of its restricted host range. In this study, JCV replication was examined using different clones in 293 cells. RT‐PCR assay revealed that large T antigen expression in cells transfected with IMR‐32‐adapted JCVs was significantly greater than in those transfected with Mad‐1 or CY. DNA replication assay and viral load verified that the IMR‐32‐adapted JCVs were replication‐competent in 293 cells, but not Mad‐1 or CY JCVs. These results suggest that a 293 culture system with IMR‐32‐adapted JCVs may be a useful tool for assessing replication of JCV in vitro.
Accounting and Preserving the American Way of Life
Authors: Walker Stephen P. 
Published: February 2015

AbstractResponding to calls to instate the visual in accounting history research, this study utilizes photographic images to reveal the role of accounting in the attempt to preserve an ideal (ruralism) and an institution (the family farm) in theUnited States. These elemental features of the American way of life were threatened during the interwar depression, resulting in governmental programs to secure their restoration. The analysis of official imagery reveals how the accounting prescriptions attending state intervention in agriculture were deemed conducive to the fortification of the economic, social and political foundations of agrarian living. Documentary photographs propagated the notion that accounting facilitated companionate marriage and the inclusive family. They also suggested that accounting was a focus for encouraging the communitarian endeavor, democratic participation and receptivity to state interventionism considered necessary to preserve the rural mode of living.
Spangled perch (Leiopotherapon unicolor) in the southern Murray‐Darling Basin: Flood dispersal and short‐term persistence outside its core range
Authors: Ellis Iain , Whiterod Nick , Linklater Danielle , Bogenhuber Deborah , Brown Paul , Gilligan Dean 
Published: February 2015

AbstractThe spangled perch Leiopotherapon unicolor is considered a rare vagrant in the southern Murray‐Darling Basin, Australia, due to its intolerance of the relatively cool water temperatures that prevail during winter months. This study details 1342 records of the species from 68 locations between 2010 and 2014 outside its accepted ‘core adult range’ following widespread flooding during 2010 and 2011. Although records of the species declined over 2013, L. unicolor remained resident in the southern Murray‐Darling Basin as of April 2014. The species persisted in several locations for three consecutive winters with recruitment documented at two sites. This study represents the first identification of the dispersal of large numbers of L. unicolor into the southern Murray‐Darling Basin, persistence beyond a single winter, and recruitment by the species in habitats south of its recognized ‘core adult range’. Targeted research would determine the potential for predicted environmental changes (artificially warmer drainage wetlands, climate change and greater floodplain connectivity) to facilitate longer term persistence and range expansion by the species in the southern Murray‐Darling Basin.
Interleukin‐33 exacerbates allergic bronchoconstriction in the mice via activation of mast cells
Authors: Sjöberg L. C. , Gregory J. A. , Dahlén S.‐E. , Nilsson G. P. , Adner M. 
Published: February 2015

AbstractBackgroundInterleukin‐33 (IL‐33) is implicated as an epithelium‐derived danger signal promoting Th2‐dependent responses in asthma. We hypothesized that IL‐33 might also have direct effects on mast cell‐driven allergic airway obstruction.MethodsThe effects of IL‐33 on allergic responses in the airways of sensiti‐zed mice were assessed both in vivo and ex vivo, as well as on cultured mast cells in vitro.ResultsIn vivo, the allergen‐induced increase in resistance in the conducting airways was enhanced in mice pretreated with IL‐33. Also, in the isolated airways, the allergen‐induced contractions were increased in preparations from animals subjected to intranasal IL‐33 pretreatment. These effects in vivo and ex vivo were blocked by the 5‐HT2A receptor antagonist ketanserin and absent in mice without mast cells. Likewise, the IL‐33‐induced enhancement of the allergen response was absent in isolated airways from mice lacking the IL‐33 receptor. Moreover, exposure to IL‐33 increased secretion of serotonin from allergen‐challenged isolated airways. In cultured mast cells, IL‐33 enhanced the expression of tryptophan hydroxylase 1, serotonin synthesis, and storage, as well as the secretion of serotonin following IgE receptor cross‐linking.ConclusionThese results demonstrate that IL‐33 exacerbates allergic bronchoconstriction by increasing synthesis, storage, and secretion of serotonin from the mast cell. This mechanism has implications for the development of airway obstruction in asthma.
Impact of metformin on anti‐Müllerian hormone in women with polycystic ovary syndrome: a secondary analysis of a randomized controlled trial
Authors: Madsen Helen N. , Lauszus Finn F. , Trolle Birgitta , Ingerslev Hans J. , Tørring Niels 
Published: February 2015

AbstractConclusions on the effect of metformin on circulating anti‐Müllerian hormone (AMH) levels in women with polycystic ovary syndrome (PCOS) are ambiguous. We performed a secondary analysis of a randomized, double‐blind, placebo‐controlled cross‐over trial. Fifty‐six women with hyperandrogenemic PCOS were included. Each woman served as her own control receiving a daily dose of either 1700 mg metformin or placebo for 6 months. After a 3‐month wash‐out period they received the opposite treatment. The decrease in AMH from a median of 49.5 to 46.9 pmol/L after 6 months on metformin was overall not significant (p = 0.81), nor were changes in obese women (from 49.5 to 38.2 pmol/L; p = 0.53). Comparing individual metformin/placebo AMH values, a small absolute decrease of 9.3 pmol/L (p = 0.03) was observed in obese women after 6 months relative to baseline, suggesting a trend towards decreasing values after metformin treatment, mainly in obese women.
Severe and fatal obstetric injury claims in relation to labor unit volume
Authors: Milland Maria , Mikkelsen Kim L. , Christoffersen Jens K. , Hedegaard Morten 
Published: February 2015

AbstractObjectiveTo assess possible association between the incidence of approved claims for severe and fatal obstetric injuries and delivery volume in Denmark.Design and settingA nationwide panel study of labor units.PopulationClaimants seeking financial compensation due to injuries occurring in labor units in 1995–2012.MethodsExposure information regarding the annual number of deliveries per labor unit was retrieved from the Danish National Birth Register. Outcome information was retrieved from the Danish Patient Compensation Association. Exposure was categorized in delivery volume quintiles as annual volume per labor unit: (10–1377), (1378–2016), (2017–2801), (2802–3861), (3862–6659).Main outcome measuresFive primary measures of outcome were used. Incidence rate ratios of (A) Submitted claims, (B) Approved claims, (C) Approved severe injury claims (120% degree of disability), (D) Approved fatal injury claims, and (C+D) Combined.Results1 151 734 deliveries in 51 labor units and 1872 submitted claims were included. The incidence rate ratios of approved claims overall, of approved fatal injury claims, and of approved severe and fatal injuries combined increased significantly with decreasing annual delivery volume. Face value incidence rate ratios of approved severe injuries increased with decreasing labor unit volume, but the association did not reach statistical significance.ConclusionHigh volume labor units appear associated with fewer approved and fewer fatal injury claims compared with units with less volume. The findings support the development towards consolidation of units in Denmark. A suggested option would be to tailor obstetric patient safety initiatives according to the delivery volume of individual labor units.
Response of fibroblast growth factor 21 to meal intake and insulin infusion in patients on maintenance haemodialysis
Authors: Reinhard Mark , Frystyk Jan , Jespersen Bente , Randers Else , Bibby Bo Martin , Ivarsen Per 
Published: February 2015

SummaryObjectiveTo investigate the response of serum fibroblast growth factor 21 (FGF21) to a meal and to insulin infusion in haemodialysis (HD) patients.Design and PatientsMeal study: in a crossover design, 12 nondiabetic HD patients were randomly assigned to: (1) a non‐HD day with one meal served, (2) a HD day with one meal served during HD and (3) a HD day with two meals served during and after HD, respectively. Twelve healthy controls participated in an experiment identical to the non‐HD day. Insulin infusion study: in a crossover design, 11 nondiabetic HD patients were randomly assigned to receive a 4‐h HD session with either: (1) no infusion, (2) glucose infusion or (3) glucose–insulin infusion. A meal was served 2 h before HD start.ResultsMeal study: serum FGF21 was 23‐fold higher in HD patients than controls (P < 0·001). Postprandial FGF21 decreased on all four study days (P < 0·006), but the relative reductions from baseline were significantly greater in controls (P < 0·008). Postprandial changes in FGF21 were inversely related with triglycerides (P = 0·042) and positively related with insulin‐like growth factor binding protein‐1 (IGFBP‐1) (P < 0·001). Serum FGF21 was only associated with changes in adiponectin (P = 0·001) and free fatty acids (P = 0·04) in the healthy controls.Insulin infusion study: as compared with no infusion, glucose and glucose–insulin infusion prevented the postprandial decrease in FGF21 and resulted in higher FGF21 concentrations by up to 25% (P = 0·003).ConclusionsSerum FGF21 was highly elevated in HD patients but the response of serum FGF21 to meal intake and insulin infusion seemed to be intact. Our results indicate that FGF21 may play an important role in short‐term metabolic homoeostasis.