bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution of PDZD8 to Stabilization of the Human Immunodeficiency Virus Type 1 Capsid
Authors: Guth Charles Alexander , Sodroski Joseph 
Published: February 2014

ABSTRACT

Following human immunodeficiency virus type 1 (HIV-1) entry into the host cell, the viral capsid gradually disassembles in a process called uncoating. A proper rate of uncoating is important for reverse transcription of the HIV-1 genome. Host restriction factors such as TRIM5α and TRIMCyp bind retroviral capsids and cause premature disassembly, leading to blocks in reverse transcription. Other host factors, such as cyclophilin A, stabilize the HIV-1 capsid and are required for efficient infection in some cell types. Here, we show that a heat-labile factor greater than 100 kDa in the cytoplasm of cells from multiple vertebrate species slows the spontaneous disassembly of HIV-1 capsid-nucleocapsid (CA-NC) complexes
in vitro
. We identified the PDZ domain-containing protein 8 (PDZD8) as a critical component of the capsid-stabilizing activity in the cytoplasmic extracts. PDZD8 has been previously reported to bind the HIV-1 Gag polyprotein and to make a positive contribution to the efficiency of HIV-1 infection (M. S. Henning, S. G. Morham, S. P. Goff, and M. H. Naghavi, J. Virol.
84:
8990–8995, 2010, doi:10.1128/JVI.00843–10). PDZD8 knockdown accelerated the disassembly of HIV-1 capsids in infected cells, resulting in decreased reverse transcription. The PDZD8 coiled-coil domain is sufficient for HIV-1 capsid binding, but other parts of the protein, including the PDZ domain, are apparently required for stabilizing the capsid and supporting HIV-1 infection. In summary, PDZD8 interacts with and stabilizes the HIV-1 capsid and thus represents a potentially targetable host cofactor for HIV-1 infection.

IMPORTANCE
After human immunodeficiency virus type 1 (HIV-1) gains access to the interior of the target cell, host cell factors can influence virus infection in either a positive or negative way. HIV-1 depends upon certain host cell factors to assist processes that are required for virus replication. One example of such a host factor is PDZD8. This work shows that PDZD8 helps to stabilize the HIV-1 capsid, a huge complex of the viral RNA, enzymes, and protein. When PDZD8 is prevented from interacting with the HIV-1 capsid, the capsid becomes unstable and HIV-1 infection is inhibited. These results show that PDZD8 regulates the uncoating of the HIV-1 capsid. Interfering with the interaction of PDZD8 and capsid could prove to be a useful strategy for intervening in HIV-1 infection and transmission.
Posterior shoulder dislocation while lifting weights: a missed diagnosis
Authors: Cuffolo Giulio , Coomber Ross , Burtt Simon , Gray Jim 
Published: February 2014

SummaryWe present a case of a 24-year-old man who suffered acute shoulder pain and subsequent inability to move his arm while lifting weights in the bench-press position. He attended A&E where he was examined and X-rays were performed. He was diagnosed with presumed pectoralis major tendon rupture and was discharged to fracture clinic the following day with analgesia. On review in clinic he was found to have a posterior shoulder dislocation and was taken to theatre for relocation under anaesthesia. This case report examines the mechanism, investigations and management of posterior shoulder dislocation.
Widespread skin rash following travel to South-East Asia
Authors: Creamer Andrew 
Published: February 2014

SummaryA 21-year-old man presented with a widespread, intensely pruritic skin rash, which developed acutely 5 days after returning from a backpacking holiday in Thailand. On initial presentation to his general practitioner, an erythematous, papular rash was noted in the upper right back, with smaller clusters of lesions on the buttocks and right arm. Based on the anatomical distribution of the lesions, a preliminary diagnosis of shingles was made. Over the following 2 weeks, the lesions failed to improve, and new snake-like lesions appeared. A dermatologist made the diagnosis of cutaneous larva migrans. The skin lesions rapidly resolved following oral albendazole therapy.
Safety and performance of the Penumbra Liberty stent system in a rabbit aneurysm model
Authors: Chavan Rajendra , Pons Stephen , Gupta Vikas , Hui Delilah , Bose Arani 
Published: February 2014

BackgroundEndovascular treatment of wide necked and large/giant intracranial aneurysms is challenging. The goal of this study was to evaluate the Liberty stent system in the rabbit elastase aneurysm model for 30, 90, and 180 days.MethodsThe Altes elastase model was used to create aneurysms in the right common carotid artery in New Zealand White rabbits. Safety was assessed by angiographic and histopathological data from treated wide necked aneurysms, treated subclavian arteries, and related vasculature immediately post-treatment and at scheduled end points.ResultsIn this study, stenting of wide necked aneurysms demonstrated excellent results in all 35 animals. The Liberty could be tracked to the final position across an aneurysm neck precisely, without changing microcatheter position. After device deployment, no significant changes in vessel contour and no stent migration were observed. Coil mass was well supported in all aneurysms and high packing densities were achieved. In addition, follow-up angiograms showed stable coil masses and persistent occlusion in all animals, without any recanalization of the neck or aneurysmal sac. Furthermore, there was no evidence of coil compaction or protrusion of coil loop through the stent struts. None of the immediate post-coiling angiograms revealed evidence of vasospasm, dissection, or in-stent thrombosis.DiscussionThis animal study demonstrated promising results with the novel Liberty stent system. The Liberty showed consistent precise positioning and accurate deployment. The stent revealed good compatibility with embolic coiling procedures, while morbidity and mortality were negligible. In addition, persistent occlusion of aneurysms without recanalization or in-stent stenosis was observed at the 180 day follow-up.
Computation of the effective slip of rough hydrophobic surfaces via homogenization
Authors: Bonnivard Matthieu , Dalibard Anne-Laure , Gérard-Varet David 
Published: February 2014

We present a quantitative analysis of the effect of rough hydrophobic surfaces on viscous Newtonian flows. We use a model introduced by Ybert and coauthors in [Achieving large with superhydrophobic surfaces: Scaling laws for generic geometries, Phys. Fluids 19 (2007) 123601], in which the rough surface is replaced by a flat plane with alternating small areas of slip and no-slip. We investigate the averaged slip generated at the boundary, depending on the ratio between these areas. This problem reduces to the homogenization of a nonlocal system, involving the Dirichlet to Neumann map of the Stokes operator, in a domain with small holes. Pondering on the works of Allaire [Homogenization of the Navier–Stokes equations in open sets perforated with tiny holes. I. Abstract framework, a volume distribution of holes, Arch. Rational Mech. Anal. 113 (1990) 209–259; Homogenization of the Navier–Stokes equations in open sets perforated with tiny holes. II. Noncritical sizes of the holes for a volume distribution and a surface distribution of holes, Arch. Rational Mech. Anal. 113 (1990) 261–298]. We compute accurate scaling laws of the averaged slip for various types of roughness (riblets, patches). Numerical computations complete and confirm the analysis.
Quantifying the impacts of climate change on groundwater in an unconfined aquifer that is strongly influenced by surface water
Authors: Scibek J. , Allen D. M. , Whitfield P. H. 
Published: February 2014

Abstract
A three-dimensional transient groundwater flow model, implemented in MODFLOW, is used to quantify the impacts of climate change on groundwater in an unconfined aquifer with demonstrated strong connection to surface water (Kettle and Granby Rivers). The Grand Forks aquifer is located in a semi-arid region of south-central British Columbia, Canada. Distributed recharge is modelled using HELP, driven by the LARS-WG stochastic weather generator, and stage–discharge curves for rivers are modelled using BRANCH and calibrated to historical data. For recharge modelling, three year-long climate scenarios were run, each representing one typical year in the present, and future (2020s and 2050s), by perturbing the historical weather according to the downscaled CGCM1 global climate model results. By the 2050s the largest increase in recharge relative to present occurs in late spring, by a factor of three or more, a 50% increase in summer months in most areas of the aquifer, a 10–25% increase in autumn, and a reduction in recharge in winter. CGCM1 downscaling was also used to predict basin-scale runoff for the Kettle River. Future climate scenarios suggest a shift in the hydrograph peak to an earlier date, although the peak flow remains the same, and baseflow level is lower and of longer duration. Groundwater levels near the river floodplain are predicted to be higher earlier in the year due to an earlier onset of peak flow, but considerably lower during the summer months. Away from rivers, groundwater levels increase slightly due to the predicted increase in recharge.
Historical damage and earthquake environmental effects related to shallow intraplate seismicity of central western Argentina
Authors: Moreiras Stella Maris , Páez María Solange 
Published: February 2014

Abstract

Central western Argentina is identified as the most hazardous seismic zone in the country. Historical earthquakes with magnitudes greater than M
s
>6.0 frequently occur in this territory and are associated with the subduction of the Nazca plate. However, seismic hazards have not been fully assessed in this region. No secondary seismic effects of a potential earthquake with destructive consequences have been considered, nor has the existence of shallow Quaternary blind faults been identified by seismic surveys. Neotectonic studies performed up to the present describe only those Quaternary faults with some surficial expression. Lacking proper hazard assessment limits strategies to reduce the economic impact of drastic seismic events. This chapter is focused on the impact of major destructive earthquakes that have occurred in central western Argentina in order to understand the incidence of these phenomena in the past and to consider the vulnerability of the region in light of its increased urbanization and changing agricultural practices.
Inhibition of Tumor Growth and Angiogenesis by SP-2, an Anti–Lectin, Galactoside-Binding Soluble 3 Binding Protein (LGALS3BP) Antibody
Authors: Traini Sara , Piccolo Enza , Tinari Nicola , Rossi Cosmo , La Sorda Rossana , Spinella Francesca , Bagnato Anna , Lattanzio Rossano , D'Egidio Maurizia , Di Risio Annalisa , Tomao Federica , Grassadonia Antonino , Piantelli Mauro , Natoli Clara , Iacobelli Stefano 
Published: February 2014

Abstract
Accumulating evidence indicates that serum and tissue levels of lectin, galactoside-binding soluble 3 binding protein (LGALS3BP), a secreted glycoprotein, are elevated in human cancers. Recently, we have identified LGALS3BP as a factor capable of stimulating angiogenesis of microvascular endothelial cells in vitro as well as in vivo. However, the potential therapeutic implications of LGALS3BP function blockade have not been explored yet. Here, we tested the ability of an anti-LGALS3BP mouse monoclonal antibody, SP-2, to antagonize LGALS3BP-induced angiogenesis and tumor growth. The antibody was found to inhibit endothelial cell tubulogenesis induced by either conditioned medium of breast cancer and melanoma cells or human recombinant LGALS3BP. In addition, SP-2 inhibited phosphorylation of FAK and its recruitment to membrane sites as well as AKT and ERK phosphorylation promoted by LGALS3BP. When used in vivo, the antibody restrained LGALS3BP-stimulated angiogenesis and growth of tumor xenografts. Furthermore, the combination of SP-2 and low-dose bevacizumab was more effective than either agent alone. Taken together, these results lead to consideration of SP-2 as a promising candidate for LGALS3BP-targeted therapy. Mol Cancer Ther; 13(4); 916–25. ©2014 AACR.
Monensin Inhibits Canonical Wnt Signaling in Human Colorectal Cancer Cells and Suppresses Tumor Growth in Multiple Intestinal Neoplasia Mice
Authors: Tumova Lucie , Pombinho Antonio R. , Vojtechova Martina , Stancikova Jitka , Gradl Dietmar , Krausova Michaela , Sloncova Eva , Horazna Monika , Kriz Vitezslav , Machonova Olga , Jindrich Jindrich , Zdrahal Zbynek , Bartunek Petr , Korinek Vladimir 
Published: February 2014

Abstract
The Wnt signaling pathway is required during embryonic development and for the maintenance of homeostasis in adult tissues. However, aberrant activation of the pathway is implicated in a number of human disorders, including cancer of the gastrointestinal tract, breast, liver, melanoma, and hematologic malignancies. In this study, we identified monensin, a polyether ionophore antibiotic, as a potent inhibitor of Wnt signaling. The inhibitory effect of monensin on the Wnt/β-catenin signaling cascade was observed in mammalian cells stimulated with Wnt ligands, glycogen synthase kinase-3 inhibitors, and in cells transfected with β-catenin expression constructs. Furthermore, monensin suppressed the Wnt-dependent tail fin regeneration in zebrafish and Wnt- or β-catenin–induced formation of secondary body axis in Xenopus embryos. In Wnt3a-activated HEK293 cells, monensin blocked the phoshorylation of Wnt coreceptor low-density lipoprotein receptor related protein 6 and promoted its degradation. In human colorectal carcinoma cells displaying deregulated Wnt signaling, monensin reduced the intracellular levels of β-catenin. The reduction attenuated the expression of Wnt signaling target genes such as cyclin D1 and SP5 and decreased the cell proliferation rate. In multiple intestinal neoplasia (Min) mice, daily administration of monensin suppressed progression of the intestinal tumors without any sign of toxicity on normal mucosa. Our data suggest monensin as a prospective anticancer drug for therapy of neoplasia with deregulated Wnt signaling. Mol Cancer Ther; 13(4); 812–22. ©2014 AACR.
Implications of glycoprotein VI for theranostics
Authors: Vogel Sebastian , Pfannenberg Christina , Pichler Bernd , Langer Harald , Bigalke Boris , Gawaz Meinrad 
Published: February 2014

SummaryGlycoprotein VI (GPVI), a membrane glycoprotein solely expressed in platelets and megakaryocytes, plays a critical role in thrombus formation due to collagen/GPVI-mediated platelet activation and adhesion. Recent studies have shown that surface expression of GPVI on circulating platelets is enhanced in acute cardiovascular diseases such as myocardial infarction and ischaemic stroke. Increased GPVI levels are associated with poor clinical outcome and are an early indicator for imminent myocardial infarction in patients with chest pain. The soluble form of the dimeric GPVI fusion protein (sGPVI-Fc) binds with high affinity to collagen and atherosclerotic plaque tissue. Non-invasive imaging studies with radiolabelled sGPVI-Fc show specific binding activity to vascular lesions in vivo. Further, sGPVI-Fc has been developed as a new therapeutic platelet-based strategy for lesion-directed antithrombotic therapy. This review summarises the potential of GPVI for diagnostic and therapeutic options based on novel non-invasive molecular imaging modalities to ameliorate care of patients with cardiovascular diseases.
New oral anticoagulants for stroke prevention in atrial fibrillation: impact of study design, double counting and unexpected findings on interpretation of study results and conclusions
Authors: Paikin Jeremy S. , Hirsh Jack , Lauw Mandy N. , Eikelboom John W. , Ginsberg Jeffrey S. , Chan Noel C. 
Published: February 2014

SummaryFour recently introduced new oral anticoagulants (dabigatran, rivaroxaban, apixaban and edoxaban) have been shown to be at least as efficacious and safe as warfarin for stroke prevention in patients with atrial fibrillation in their respective trials. The first three have been approved, while edoxaban is awaiting regulatory approval. Several guidelines have endorsed the approved new oral anticoagulants over warfarin because of their favourable risk-benefit ratio, low propensity for food and drug interactions, and lack of requirement for routine coagulation monitoring. In this invited review, we summarise the results of the four studies and discuss widely held conclusions. We take a step further and discuss how differences in study design, analysis plan, and unexpected events affect the interpretation of the study results. Finally, we take our re-interpretation of study results and discuss how they might impact clinical practice and anticoagulant choice for patients.
Circulating microparticles: challenges and perspectives of flow cytometric assessment
Authors: Shantsila Eduard , Montoro-García Silvia , Gallego Pilar , Lip Gregory Y. H. 
Published: February 2014

SummaryCirculating blood microparticles are likely to play a significant role as messengers of biological information. Their accurate quantification and characterisation is challenging and needs to be carefully designed with preferable usage of fresh minimally-processed blood samples. Utilisation of flow cytometers specifically designed for analysis of small-size particles is likely to provide considerable methodological advantages and should be the preferable option. This viewpoint manuscript provides a critical summary of the key methodological aspects of microparticle analysis.Note: The review process for this viewpoint article was fully handled by Christian Weber, Editor in Chief.
The Annual Rhythm of Activity of the Lateral Meristems (Cambium and Phellogen) in Pinus Halepensis Mill. and Pinus Pinea L.
Authors: Liphschitz Nili , Lev-Yadun S. , Rosen E. , Waisel Y. 
Published: February 2014

The annual rhythms of cambial and phellogen activity in Pinus halepensis and P pinea were investigated. Under natural conditions the cambium of P halepensis begins its activity in autumn, enters a quiescent period during midwinter, resurnes activity towards spring and enters a second rest period in summer. The ring border is formed during summer. Irrigated plants growing outdoors were active almost all the year round.The cambium of P pinea is active between April and November and enters a true winter dormancy.The duration of xylem production exceeded that of the phloem. More xylem than phloem cells were formed. The phellogen was active during a short period only.Pinus halepensis seems to follow the Mediterranean climate patterns whereas P pinea follows the pattern of a colder climate.
Vestures and Thickenings of the Vessel Wall in Some Species of Prosopis (Leguminosae)
Authors: Agueda Castro María 
Published: February 2014

In the present paper vestured pits, vestures on the thickenings of the vessel walls, and the warty layer in the wood of some speeies of Prosopis (Leguminosae - Mimosoideae) are reported.The occurrenee of fibres with a G-layer (gelatinous fibres) is also documented.
Anatomy and Properties of Japanese Hardwoods I. Variation of Fibre Dimensions and Tissue Proportions and their Relation to Basic Density
Authors: Fujiwara Shinji , Sameshima Kazuhiko , Kuroda Kenichi , Takamura Norio 
Published: February 2014

Variations of tissue proportions and fibre dimensions (fibre length, fibre cross sectional area, fibre wall thickness, fibre wall area, percentage of fibre wall area) of 50 Japanese hardwoods are compared. The fibre cross sectional area showed large variations as compared to fibre length. There is no significant correlation between fibre cross sectional area and fibre wall thickness.The multiple regression analysis showed that fibre wall thickness, percentage of fibre wall material and ray volume are the parameters most closely correlated with basic density.
Ultrastructure of Vestures on the Vessel Wall in Some Species of Prosopis (Leguminosae-Mimosoideae)
Authors: Castro María Agueda 
Published: February 2014

Ultrastructural characteristics of the vessel wall of fourteen Argentinian species of Prosopis L. were studied with SEM and TEM.Branched and 'wart-like' vestures are present on the inner surface of the vessel. TEM observations permitted to define them as outgrowths of the secondary wall covered by an electron dense layer, probably composed of polysaccharides.
Posterior Tibial Slope Influences Static Anterior Tibial Translation in Anterior Cruciate Ligament Reconstruction
Authors: Li Yue , Hong Lei , Feng Hua , Wang Qianqian , Zhang Jin , Song Guanyang , Chen Xingzuo , Zhuo Hongwu 
Published: February 2014

Background: Posterior tibial slope (PTS) has recently been identified as a risk factor for anterior cruciate ligament (ACL) injuries because of an associated increase in anterior tibial translation (ATT) and ACL loading. However, few studies concerning the correlation between PTS and postoperative ATT have been published. Purpose: To analyze the relationship between PTS and postoperative ATT in ACL reconstruction (ACLR). Study Design: Case control study; Level of evidence, 3. Methods: Included in this retrospective study were 40 consecutive patients who underwent ACLR (28 male, 12 female; median age, 22 years; range, 14-44 years) from October 2010 to June 2011. The patients were divided into 3 groups based on medial and lateral PTS values as measured on MRI. Demographic data and results of the manual maximum side-to-side difference with a KT-1000 arthrometer at 30° of knee flexion before ACLR and at final follow-up were collected; results were divided into ATT ≤2 mm, 2 mm < ATT < 5 mm, and ATT ≥5 mm. First, the distribution of ATT in the 3 groups was compared, and then correlation analysis and logistic regression were conducted to determine the correlation between PTS and ATT. Finally, the thresholds of medial and lateral PTS were calculated. Results: Results of the ATT measurements were collected at a mean of 27.5 months (range, 24.0-37.0 months) after ACLR. The group with a PTS ≥5° had significantly more cases of ATT ≥5 mm than the group with a PTS <3° (medial PTS: P = .005; lateral PTS: P = .016). There were statistically significant correlations with ATT for both medial ( r = 0.43, P = .005) and lateral ( r = 0.36, P = .02) PTS. Medial or lateral PTS resulted in the increased probability of ATT ≥5 mm, with an odds ratio of 1.76 ( P = .011) and 1.68 ( P = .008), respectively. The threshold of an increased risk of ATT ≥5 mm was a medial PTS >5.6° ( P = .003) or a lateral PTS >3.8° ( P = .002). Conclusion: There was a significant correlation between PTS and postoperative anterior knee static stability in this study. Patients with a steeper medial or lateral PTS showed a higher risk of ATT ≥5 mm at thresholds of 5.6° and 3.8°, respectively.