bluuebunny/crossref_metadata_2025_split huggingface dataset:
First insights of integrating the Bonn Internship Curriculum for Point-of-Care Ultrasound (BI-POCUS): progress and educational aspects
Authors: Höhne Elena , Schäfer Valentin Sebastian , Petzinna Simon Michael , Wittek Agnes , Gotta Jennifer , Reschke Philipp , Recker Florian 
Published: August 2024

Abstract
Background
Point-of-care ultrasound (POCUS) is rapidly gaining prominence in various clinical settings. As its use becomes more widespread, there is a growing need for comprehensive ultrasound training in medical education to ensure that future healthcare professionals are proficient in this essential diagnostic tool.

Objective
This study is the first attempt by the University of Bonn to seamlessly integrate ultrasound courses and the use of ultrasound devices into the regular activities of final year medical students and to evaluate the usage of these devices.

Methods
A total of forty students in their practical year were provided with a hendheld ultrasound device for a period of four months. During this time, they were invited to take part in eight optional ultrasound courses in which they acquired images and those images were rated using a specially developed rating system. At the end of the tertial, students were able to take part in a voluntary survey on the use of the equipment.

Results
Participation in the optional ultrasound courses was well received, with the Introduction and FAST module drawing the largest number of participants (29). Among the ultrasound images acquired by students, those of the lungs obtaining the highest rating, with 18.82 (SD ± 4.30) points out of 23 points, while the aorta and vena cava images scored lowest, with an average of 16.62 (SD ± 1.55) points. The overall mean score for all images was 17.47 (SD ± 2.74). Only 21 students responded to the survey. Of the participating students, 67% used the device independently four times or fewer during the tertial.

Conclusion
The study aimed to enhance the BI-POCUS curriculum by improving students' ultrasound skills during their practical year. However, device usage was lower than expected, with most students using it only once a month or less. This raises concerns about the justification of the effort and resources. Future initiatives will focus on technical improvements, better login data provision, and closer monitoring of usage and progress, emphasizing the need for practical ultrasound training in medical education.
Migrant workers’ perception and awareness of health insurance coverage in Brunei Darussalam
Authors: Lai Alice , Mohammad Noorfaizah , Trivedi Ashish , Murang Zaidah , Tuah Nik 
Published: August 2024

Abstract
Background
Migrant workers are recognized as a vulnerable group of population in the context of accessibility to healthcare services as they are likely to experience multiple challenges and barriers. This study aimed to assess the awareness and perceived knowledge on health insurance coverage among documented migrant workers in Brunei Darussalam.

Methods
This cross-sectional, interviewer-administered study used a pre-designed questionnaire on migrant workers attending the Foreign Worker Health Screening Centre from June until September 2019. Data were analyzed for association between perceived insurance status on health-seeking behavior.

Results
The study obtained responses from 469 documented migrant workers (93.8%). 75.1% reported being aware of and having health insurance coverage; and of these, 57.1% were aware of the type of health insurance cover they had. 45.5% and 50.6% had poor knowledge whether their health insurance covered for hospitalization or outpatient expenses, respectively. No significant association was found between the migrant workers’ perceived status of insurance and not seeking medical care due to financial barriers (p > 0.05).

Conclusions
A high proportion of documented migrant workers in Brunei Darussalam reported knowledge of having health insurance; however, there was lack of awareness on its actual coverage. By including migrants’ health in a nation’s healthcare governance, the health rights of migrant workers can be addressed thus aiming to achieve universal health coverage for all individuals.
Protocol for a randomized controlled trial of an internet-based prevention intervention for young children at-risk for functional abdominal pain
Authors: Levy Rona L. , Murphy Tasha B. , van Tilburg Miranda A. L. , Kuklinski Margaret R. , Bailey Jennifer A. , Aalfs Homer , Badillo Isabel , Diakhate Hafsah , Palermo Tonya M. 
Published: August 2024

Abstract

Background
Chronic pain often clusters in families, where parents and their offspring both experience chronic pain conditions. Young children of parents with irritable bowel syndrome (IBS) represent an at-risk group for the development of abdominal pain, disability, and excess health care visits in later childhood. Parental solicitous responses to children’s expressions of discomfort and maternal modeling of their own illness behavior contribute to a greater focus on somatic sensations, leading to illness behaviors in children. This randomized controlled trial will test the effectiveness of an early preventive web-based psychosocial intervention (REACH)[TM] vs. an educational web-based safety comparison condition delivered to parents with IBS to alter parental responses and lead to improved child health and decreased health care costs.

Methods
Parents with IBS who have children ages 4–7 years are recruited via community-based approaches (e.g., social media advertisements, school electronic distribution, research networks) and health care providers. The target sample is 460 parents randomized to REACH, a web-based social learning and cognitive behavior therapy (SLCBT) intervention or an educational web-based safety comparison condition (EC). Participants will be assessed at baseline, 6-week (immediate post-intervention), 6-month, 12-month, and 18-month follow-up periods (months post-completion of intervention). The primary outcome is change in parental solicitous/protective behaviors. Secondary outcomes include parent risk and protective factors, child health and symptom outcomes, and health care utilization and cost savings.

Discussion
This study adapts a validated, parent-delivered intervention to treat chronic pain in children to a web-based application designed to prevent the development of chronic pain in very young, high-risk children. If successful, this strategy can both prevent adverse sequelae of this condition from developing as well as be widely accessible. Furthermore, the availability of a prevention model for parent training could result in significant short- and long-term health benefits across a broad spectrum of conditions.

Trial registration
ClinicalTrials.gov NCT05730491. Registered on February 15, 2023.
SOX2 interacts with hnRNPK to modulate alternative splicing in mouse embryonic stem cells
Authors: Huang Yanlan , Liu Yuxuan , Pu Mingyi , Zhang Yuli , Cao Qiang , Li Senru , Wei Yuanjie , Hou Linlin 
Published: August 2024

Abstract
Background
SOX2 is a determinant transcription factor that governs the balance between stemness and differentiation by influencing transcription and splicing programs. The role of SOX2 is intricately shaped by its interactions with specific partners. In the interactome of SOX2 in mouse embryonic stem cells (mESCs), there is a cohort of heterogeneous nuclear ribonucleoproteins (hnRNPs) that contributes to multiple facets of gene expression regulation. However, the cross-talk between hnRNPs and SOX2 in gene expression regulation remains unclear.

Results
Here we demonstrate the indispensable role of the co-existence of SOX2 and heterogeneous nuclear ribonucleoprotein K (hnRNPK) in the maintenance of pluripotency in mESCs. While hnRNPK directly interacts with the SOX2-HMG DNA-binding domain and induces the collapse of the transcriptional repressor 7SK small nuclear ribonucleoprotein (7SK snRNP), hnRNPK does not influence SOX2-mediated transcription, either by modulating the interaction between SOX2 and its target cis-regulatory elements or by facilitating transcription elongation as indicated by the RNA-seq analysis. Notably, hnRNPK enhances the interaction of SOX2 with target pre-mRNAs and collaborates with SOX2 in regulating the alternative splicing of a subset of pluripotency genes.

Conclusions
These data reveal that SOX2 and hnRNPK have a direct protein-protein interaction, and shed light on the molecular mechanisms by which hnRNPK collaborates with SOX2 in alternative splicing in mESCs.
The tripartite motif-containing 24 is a multifunctional player in human cancer
Authors: Yao Yuanbing , Zhou Sheng , Yan Yue , Fu Kai , Xiao Shuai 
Published: August 2024

AbstractTripartite motif-containing 24 (TRIM24), also known as transcriptional intermediary factor 1α (TIF1α), is the founding member of TIF1 family. Recent evidence indicates that aberrant expression of TRIM24, functions as an oncogene, is associated with poor prognosis across various cancer types. TRIM24 exhibits a multifaceted structure comprising an N-terminal TRIM region with a RING domain, B-box type 1 and type 2 domains, and a coiled-coil region, as well as a C-terminal plant-homeodomain (PHD)-bromodomain. The bromodomain serves as a ‘reader’ of epigenetic histone marks, regulating chromatin structure and gene expression by linking associated proteins to acetylated nucleosomal targets, thereby controlling transcription of genes. Notably, bromodomains have emerged as compelling targets for cancer therapeutic development. In addition, TRIM24 plays specialized roles as a signal transduction molecule, orchestrating various cellular signaling cascades in cancer cells. Herein, we review the recent advancements in understanding the functions of TRIM24, and demonstrate the research progress in utilizing TRIM24 as a target for cancer therapy.
Trajectory of brain-derived amyloid beta in Alzheimer’s disease: where is it coming from and where is it going?
Authors: Liu Ni , Haziyihan Anaer , Zhao Wei , Chen Yu , Chao Hongbo 
Published: August 2024

AbstractAlzheimer’s disease (AD) is a progressive neurological disorder that primarily impacts cognitive function. Currently there are no disease-modifying treatments to stop or slow its progression. Recent studies have found that several peripheral and systemic abnormalities are associated with AD, and our understanding of how these alterations contribute to AD is becoming more apparent. In this review, we focuse on amyloid‑beta (Aβ), a major hallmark of AD, summarizing recent findings on the source of brain-derived Aβ and discussing where and how the brain-derived Aβ is cleared in vivo. Based on these findings, we propose future strategies for AD prevention and treatment, from a novel perspective on Aβ metabolism.
Efficacy of NKG2D CAR-T cells with IL-15/IL-15Rα signaling for treating Epstein-Barr virus-associated lymphoproliferative disorder
Authors: Mai Qiusui , He Bailin , Deng Shikai , Zeng Qing , Xu Yanwen , Wang Cong , Pang Yunyi , Zhang Sheng , Li Jinfeng , Zeng Jinfeng , Huang Liqin , Fu Yongshui , Li Chengyao , Li Tingting , Xu Xiaojun , Zhang Ling 
Published: August 2024

AbstractEpstein-Barr virus (EBV) related post-transplant lymphoproliferative disorder (EBV-PTLD) is a life-threatening complication after hematopoietic stem cell transplantation (HSCT) or solid organ transplantation (SOT), for which no standard therapeutic means have been developed. Significant increase expression of natural killer group 2 member D ligands (NKG2DLs) was observed on B-lymphoblastoid cells of EBV-PTLD, indicating NKG2DLs as potential therapeutic targets for treatment of EBV-PTLD. In this study, the recombinant constructs of NKG2D CAR and IL-15/IL-15Rα-NKG2D CAR were generated with a retroviral vector and then transduced to human T cells to produce NKG2D CAR-T and IL-15/IL-15Rα-NKG2D CAR-T cells, respectively. B-lymphoblastoid cell lines (B-LCLs) and the xenografted mouse models were established to evaluate the efficacy of these CAR-T cells. IL-15/IL-15Rα-NKG2D CAR-T cells exhibited superior proliferation and antigen-specific cytotoxic effect compared to NKG2D CAR-T, as IL-15/IL-15Rα signaling promoted the expansion of less differentiated central memory T cells (TCM) and increased expression of CD107a and IFN-γ. Moreover, EBV DNA load was dramatically reduced, and 80% B-LCL cells were eliminated by IL-15/IL-15Rα-NKG2D CAR-T cells after co-culturing. In-vivo study confirmed that IL-15/IL-15Rα-NKG2D CAR-T cell therapy significantly enhanced antiviral efficacy in mice, as the serum load of EBV after IL-15/IL-15Rα-NKG2D CAR-T cell infusion was 1500 times lower than the untreated control (P < 0.001). The enhanced efficacy of IL-15/IL-15Rα-NKG2D CAR T cells was probably due to the IL-15/IL-15Rα signaling improved homing and persistence of NKG2D CAR-T cells in vivo, and increased the production of IFN-γ, Perforin, and Granulysin. In conclusion, NKG2D CAR-T cells co-expressing IL-15/IL-15Rα promoted the central memory CAR T cell proliferation and improved the homing and persistence of CAR T cells in vivo, resulting in enhanced anti-tumor and anti-viral effects in treating EBV-PTLD.
Epigenetic alterations of TP53INP1 by EHMT2 regulate the cell cycle in gastric cancer
Authors: Ryu Tae Young , Tae In Hwan , Han Tae-Su , Lee Jinkwon , Kim Kwangho , Kang Yunsang , Kim Solbi , Lee Hyo Jin , Jung Cho-Rok , Lim Jung Hwa , Kim Dae-Soo , Son Mi-Young , Cho Hyun-Soo 
Published: August 2024

Abstract
Background
Gastric cancer (GC) is a type of cancer with high incidence and mortality rates. Although various chemical interventions are being developed to treat gastric cancer, there is a constant demand for research into new GC treatment targets and modes of action (MOAs) because of the low effectiveness and side effects of current treatments.

Methods
Using the TCGA data portal, we identified EHMT2 overexpression in GC samples. Using RNA-seq and EHMT2-specific siRNA, we investigated the role of EHMT2 in GC cell proliferation and validated its function with two EHMT2-specific inhibitors. Through the application of 3D spheroid culture, patient-derived gastric cancer organoids (PDOs), and an in vivo model, we confirmed the role of EHMT2 in GC cell proliferation.

Results
In this study, we found that EHMT2, a histone 3 lysine 9 (H3K9) methyltransferase, is significantly overexpressed in GC patients compared with healthy individuals. Knockdown of EHMT2 with siRNA induced G1 cell cycle arrest and attenuated GC cell proliferation. Furthermore, we confirmed that TP53INP1 induction by EHMT2 knockdown induced cell cycle arrest and inhibited GC cell proliferation. Moreover, specific EHMT2 inhibitors, BIX01294 and UNC0638, induced cell cycle arrest in GC cell lines through TP53INP1 upregulation. The efficacy of EHMT2 inhibition was further confirmed in a 3D spheroid culture system, PDOs, and a xenograft model.

Conclusions
Our findings suggest that EHMT2 is an attractive therapeutic target for GC treatment.
Participatory intention and behavior in green cultural heritage conservation: an application of the extended theory of planned behavior
Authors: Xie Jing , Li Hongyu , Furuya Katsunori , Chen Jie , Luo Shixian 
Published: August 2024

Abstract
Cultural heritage is an important contributor to sustainable urban development, and its conservation is considered a global task. Previous research has primarily focused on the conservation of heritage buildings within architectural contexts and world heritage sites within geographical contexts. However, there is a notable gap in conservation of green cultural heritage (GCH) within a landscape-based framework. This study used the extended theory of planned behavior to explore the factors and pathways that influence the public's behavioral intentions toward GCH conservation. Based on 1075 questionnaires collected in Tokyo, Japan, the hypotheses of the newly constructed theoretical model were tested using partial least squares structural equation modeling. The results show that perceived behavioral control has the greatest positive influence on citizens' GCH conservation intentions and behaviors, while social norms do not influence people's behavioral intention to conserve. In the extended factors, the environmental awareness which including heritage awareness and cultural attachment, significantly influences public attitudes towards conservation. Additionally, environmental perception, including perceived usefulness and perceived quality, significantly affects the public's conservation intentions and behaviors, respectively. Our findings have implications for local governments and policymakers to enhance public participation in GCH, as well as some several new theoretical interests for further studies.
The development of a new oral health patient reported outcome measure: the New South Wales public dental services approach
Authors: Chen Rebecca , Ajwani Shilpi , Christian Bradley , Phelan Claire , Srinivas Ravi , Kenny Josephine , O’Connor Mark , Clarke Kara , Sohn Woosung , Yaacoub Albert 
Published: August 2024

Abstract
Background
Addressing Patient Reported Outcomes (PROs) is essential for patient-centred care, shared decision making and improved health outcomes. Value-based health care systems in New South Wales (NSW) have a growing focus on collecting and using PROs that matter most to patients to improve their healthcare outcomes. Developing oral health patient reported outcomes measures (OH-PROM) is a first step towards value-based oral health care. This paper describes the development process of an adult and child OH-PROM tool that can be piloted for NSW public dental patients.

Methods
An expert panel was assembled to undertake a systematic process of developing OH-PROMs for NSW Health. Key methodological considerations included: (1) forming an expert panel to specify the target population and context of implementation, (2) rapid literature review and environmental scan to identify existing validated OH-PROM tools for adults and children. (3) consensus gathering with the expert panel (4) consumer feedback, and (5) finalisation of the tool for electronic oral health record (eOHR) integration to establish a set of questions, that were relevant, context-appropriate, and important to oral healthcare outcomes for patients using public dental services.

Results
The panel considered a total of 59 questions from two child (15), and four adult (44) Oral Health Related Quality of Life (OHRQoL) questionnaires used to collect OH-PROMs. These questions were mapped to the four key dimensions of OHRQoL for OH-PROMs: Oral Function, Orofacial Pain, Orofacial Appearance, and Psychosocial Impact. The consensus resulted in seven questions that aligned with these four dimensions to form two new NSW OH-PROM tools: one for adults and one for children. The tools were tested with consumers for understandability and usefulness before being incorporated into the electronic oral health record system, in readiness for future pilot testing.

Conclusion
The process for developing new OH-PROMs for NSW public dental services took a pragmatic approach that combined literature appraisal, expert consensus, and consumer consultation. Future work will assess the implementation of the OH-PROM tool and test its validity for broader use as an outcome measure for value-based oral healthcare.
Potato: from functional genomics to genetic improvement
Authors: Qu Li , Huang Xueqing , Su Xin , Zhu Guoqing , Zheng Lingli , Lin Jing , Wang Jiawen , Xue Hongwei 
Published: August 2024

AbstractPotato is the most widely grown non-grain crop and ranks as the third most significant global food crop following rice and wheat. Despite its long history of cultivation over vast areas, slow breeding progress and environmental stress have led to a scarcity of high-yielding potato varieties. Enhancing the quality and yield of potato tubers remains the ultimate objective of potato breeding. However, conventional breeding has faced challenges due to tetrasomic inheritance, high genomic heterozygosity, and inbreeding depression. Recent advancements in molecular biology and functional genomic studies of potato have provided valuable insights into the regulatory network of physiological processes and facilitated trait improvement. In this review, we present a summary of identified factors and genes governing potato growth and development, along with progress in potato genomics and the adoption of new breeding technologies for improvement. Additionally, we explore the opportunities and challenges in potato improvement, offering insights into future avenues for potato research.
Experimental behavior of connection between composite columns and reinforced concrete flat slab
Authors: Yousry Mohamed , Mostafa Hatem , Youssef Ahmed 
Published: August 2024

AbstractThe combination of concrete-filled steel tube (CFST) columns and reinforced concrete flat slabs provides a suitable structural solution that can be used to replace traditional structures in high-rise buildings. The connection between the CFST column and the RC flat slab is an important factor for this construction system to operate efficiently. In this study, a new form for the connection between the CFST column and the RC flat slab has been proposed. This connection has more constructability, where the steel tube is cut at the level of the slab to prevent the separation between the slab and the concrete inside the steel tube as the slab reinforcement is allowed to continue in the connection zone. The joint zone is strengthened with rebar rings to compensate for the loss in confinement caused by cutting the steel tube at the slab level. In addition to using longitudinal rebar in the joint zone to bear part of the vertical load and to ensure bending rigidity. The behavior of the proposed connection was investigated by testing three sets of small-sized samples. In addition, an equation based on the outcomes of the second set of tests has been carried out to determine the joint bearing capacity for the vertical load.
Optimizing scientific workflow scheduling in cloud computing: a multi-level approach using whale optimization algorithm
Authors: Zhang Xiaowen 
Published: August 2024

AbstractCloud computing has evolved into an indispensable tool for facilitating scientific research due to its ability to efficiently distribute and process workloads in a virtual environment. Scientific tasks that involve complicated task dependencies and user-defined constraints related to quality of service (QoS) and time constraints require the efficient use of cloud resources. Planning these scientific workflow tasks represents an NP-complete problem, prompting researchers to explore various solutions, including conventional planners and evolutionary optimization algorithms. In this study, we present a novel, multistage algorithm specifically designed to schedule scientific workflows in cloud computing contexts. This approach addresses the challenges of efficiently mapping complex workflows onto distributed cloud resources while considering factors like resource heterogeneity, dynamic workloads, and stringent performance requirements. The algorithm uses the whale optimization algorithm (WOA) with a two-phase approach to shorten execution time, minimize financial costs, and effectively maintain load balancing.