bluuebunny/crossref_metadata_2025_split huggingface dataset:
Draft Genome Sequence of an Extensively Drug-Resistant Acinetobacter baumannii Indigo-Pigmented Strain
Authors: Traglia German , Vilacoba Elisabet , Almuzara Marisa , Diana Leticia , Iriarte Andres , Centrón Daniela , Ramírez María Soledad 
Published: November 2014

ABSTRACT

Last year in 2013, we reported an outbreak due to indigo-pigmented
Acinetobacter baumannii
strains in a hospital from Buenos Aires, Argentina. Here, we present the draft genome sequence of one of the strains (
A. baumannii
A33405) involved in the outbreak. This isolate was categorized as extensively drug-resistant (XDR) and harbors different genetic elements associated with horizontal genetic transfer and multiple antibiotic resistances.
Complete Nucleotide Sequence of a Citrobacter freundii Plasmid Carrying KPC-2 in a Unique Genetic Environment
Authors: Yao Yancheng , Imirzalioglu Can , Hain Torsten , Kaase Martin , Gatermann Soeren , Exner Martin , Mielke Martin , Hauri Anja , Dragneva Yolanta , Bill Rita , Wendt Constanze , Wirtz Angela , Domann Eugen , Chakraborty Trinad 
Published: November 2014

ABSTRACT

The complete and annotated nucleotide sequence of a 54,036-bp plasmid harboring a
bla
KPC-2
gene that is clonally present in
Citrobacter
isolates from different species is presented. The plasmid belongs to incompatibility group N (IncN) and harbors the class A carbapenemase KPC-2 in a unique genetic environment.
Genome Sequence of the Anterograde-Spread-Defective Herpes Simplex Virus 1 Strain MacIntyre
Authors: Szpara Moriah L. , Tafuri Yolanda R. , Parsons Lance , Shreve Jacob T. , Engel Esteban A. , Enquist L. W. 
Published: November 2014

ABSTRACTWe used paired-end Illumina deep sequencing andde novoassembly to determine the genome sequence of herpes simplex virus 1 (HSV-1) strain MacIntyre (aka McIntyre). The MacIntyre strain originated from the brain of a patient with lethal HSV encephalitis and has a unique limitation in its neuronal spread, moving solely in the retrograde direction.
Metagenome Sequencing of the Microbial Community of a Solar Saltern Crystallizer Pond at Cáhuil Lagoon, Chile
Authors: Plominsky Alvaro M. , Delherbe Nathalie , Ugalde Juan A. , Allen Eric E. , Blanchet Marine , Ikeda Priscila , Santibañez Francisco , Hanselmann Kurt , Ulloa Osvaldo , De la Iglesia Rodrigo , von Dassow Peter , Astorga Marcia , Gálvez María Jesús , González María Lorena , Henríquez-Castillo Carlos , Vaulot Daniel , Lopes do Santos Adriana , van den Engh Gerrit , Gimpel Carla , Bertoglio Florencia , Delgado Yolaine , Docmac Felipe , Elizondo-Patrone Claudia , Narváez Silvia , Sorroche Fernando , Rojas-Herrera Marcelo , Trefault Nicole 
Published: November 2014

ABSTRACT
Cáhuil Lagoon in central Chile harbors distinct microbial communities in various solar salterns that are arranged as interconnected ponds with increasing salt concentrations. Here, we report the metagenome of the 3.0- to 0.2-µm fraction of the microbial community present in a crystallizer pond with 34% salinity.
Sinking skin flap syndrome in glioblastoma
Authors: Kamiya-Matsuoka Carlos , Shroff Sheetal , Tatsui Claudio E , Tremont-Lukats Ivo W , Gilbert Mark R 
Published: November 2014

Sinking skin flap syndrome (SSFS) is a rare neurological complication in patients with traumatic haemorrhage, stroke or cerebral oedema who undergo decompressive craniectomy to relieve increased intracranial pressure. Hallmark of SSFS is the sinking of the scalp to a plane lower than the edges of the skull defect in the setting of neurological deterioration. Our objective is to report that SSFS can present after small craniotomy without cerebral cortex compression and to share our diagnostic/therapeutic approach. A 62-year-old woman with a glioblastoma developed SSFS after a small craniectomy and tumour resection without cerebral cortex compression but a decrease in the surgical cavity volume. Brain MRI showed decreased size of the surgical cavity. Interestingly, the patient also developed posterior reversible encephalopathy syndrome (PRES).This case highlights an atypical presentation of SSFS and the possible association with PRES. It also illustrates how an early cranioplasty can successfully reverse SSFS.
Plantaris excision in the treatment of non-insertional Achilles tendinopathy in elite athletes
Authors: Calder James D F , Freeman Richard , Pollock Noel 
Published: November 2014

BackgroundAchilles tendinopathy is a serious and frequently occurring problem, especially in elite athletes. Recent research has suggested a role for the plantaris tendon in non-insertional Achilles tendinopathy.AimTo assess whether excising the plantaris tendon improved the symptoms of Achilles tendinopathy in elite athletes.MethodsThis prospective consecutive case series study investigated 32 elite athletes who underwent plantaris tendon excision using a mini-incision technique to treat medially located pain associated with non-insertional Achilles tendinopathy. Preoperative and postoperative visual analogue scores (VAS) for pain and the foot and ankle outcome score (FAOS) as well as time to return to sport and satisfaction scores were assessed.ResultsAt a mean follow-up of 22.4 months (12–48), 29/32 (90%) of athletes were satisfied with the results. Thirty of the 32 athletes (94%) returned to sport at a mean of 10.3 weeks (5–27). The mean VAS score improved from 5.8 to 0.8 (p<0.01) and the mean FAOS improved in all domains (p<0.01). Few complications were seen, four athletes experienced short-term stiffness and one had a superficial wound infection.ConclusionsThe plantaris tendon may be responsible for symptoms in some athletes with non-insertional Achilles tendinopathy. Excision carries a low risk of complications and may provide significant improvement in symptoms enabling an early return to elite-level sports.
Do weight perceptions among obese adults in Great Britain match clinical definitions? Analysis of cross-sectional surveys from 2007 and 2012
Authors: Johnson Fiona , Beeken Rebecca J , Croker Helen , Wardle Jane 
Published: November 2014

ObjectivesTo assess the proportion of the adult obese population in Great Britain who would describe their weight using the terms ‘obese’ and ‘very overweight’ in 2007 and 2012, and identify factors associated with more accurate weight perceptions.DesignAnalysis of weight perception data from two population-based surveys.SettingPopulation surveys conducted in Great Britain.ParticipantsSurvey respondents (N=657) whose self-reported weight and height placed them in the obese category: body mass index (BMI) ≥30.Primary outcome measureSelf-identification using the terms ‘obese’ and ‘very overweight’.ResultsThe proportion of obese adults selecting the term ‘obese’ to describe their body size was very low in both women (13% in 2007 and 11% in 2012) and men (4% in 2007 and 7% in 2012) and did not change significantly. Recognition of a substantial degree of overweight (as indexed by endorsement of either of the terms ‘obese’ or ‘very overweight’) declined substantially in women, from 50% in 2007 to 34% in 2012. It was not significantly changed in men (27% in 2007 and 23% in 2012). Having a higher BMI, and being able to identify the BMI threshold for obesity were associated with self-identifying as obese or very overweight.ConclusionsThe majority of the adult obese population of Great Britain do not identify themselves as either ‘obese’ or even ‘very overweight’. Public health initiatives to tackle obesity are likely to be hampered by this lack of recognition of weight status. It is important to understand whether moves to increase personal awareness of weight status in the obese population can facilitate beneficial behaviour change, and what role health professionals can play in increasing awareness of weight status in obese patients.
PC6 acupoint stimulation for the prevention of postcardiac surgery nausea and vomiting: a protocol for a two-group, parallel, superiority randomised clinical trial
Authors: Cooke Marie , Rickard Claire , Rapchuk Ivan , Shekar Kiran , Marshall Andrea P , Comans Tracy , Doi Suhail , McDonald John , Spooner Amy 
Published: November 2014

IntroductionPostoperative nausea and vomiting (PONV) are frequent but unwanted complications for patients following anaesthesia and cardiac surgery, affecting at least a third of patients, despite pharmacological treatment. The primary aim of the proposed research is to test the efficacy of PC6 acupoint stimulation versus placebo for reducing PONV in cardiac surgery patients. In conjunction with this we aim to develop an understanding of intervention fidelity and factors that support, or impede, the use of PC6 acupoint stimulation, a knowledge translation approach.Methods and analysis712 postcardiac surgery participants will be recruited to take part in a two-group, parallel, superiority, randomised controlled trial. Participants will be randomised to receive a wrist band on each wrist providing acupressure to PC six using acupoint stimulation or a placebo. Randomisation will be computer generated, use randomly varied block sizes, and be concealed prior to the enrolment of each patient. The wristbands will remain in place for 36 h. PONV will be evaluated by the assessment of both nausea and vomiting, use of rescue antiemetics, quality of recovery and cost. Patient satisfaction with PONV care will be measured and clinical staff interviewed about the clinical use, feasibility, acceptability and challenges of using acupressure wristbands for PONV.Ethics and disseminationEthics approval will be sought from appropriate Human Research Ethics Committee/s before start of the study. A systematic review of the use of wrist acupressure for PC6 acupoint stimulation reported minor side effects only. Study progress will be reviewed by a Data Safety Monitoring Committee (DSMC) for nausea and vomiting outcomes at n=350. Dissemination of results will include conference presentations at national and international scientific meetings and publications in peer-reviewed journals. Study participants will receive a one-page lay-summary of results.Trial registration numberAustralian New Zealand Clinical Trials Registry—ACTRN12614000589684.
Governmental designation of spine specialty hospitals, their characteristics, performance and designation effects: a longitudinal study in Korea
Authors: Kim Sun Jung , Yoo Ji Won , Lee Sang Gyu , Kim Tae Hyun , Han Kyu-Tae , Park Eun-Cheol 
Published: November 2014

ObjectivesThis study compares the characteristics and performance of spine specialty hospitals versus other types of hospitals for inpatients with spinal diseases in South Korea. We also assessed the effect of the government's specialty hospital designation on hospital operating efficiency.SettingWe used data of 823 hospitals including 17 spine specialty hospitals in Korea.ParticipantsAll spine disease-related inpatient claims nationwide (N=645 449) during 2010–2012.InterventionsNo interventions were made.Outcome measuresUsing a multilevel generalised estimating equation and multilevel modelling, this study compared inpatient charges, length of stay (LOS), readmission within 30 days of discharge and in-hospital death within 30 days of admission in spine specialty versus other types of hospitals.ResultsSpine specialty hospitals had higher inpatient charges per day (27.4%) and a shorter LOS (23.5%), but per case charges were similar after adjusting for patient-level and hospital-level confounders. After government designation, spine specialty hospitals had 8.8% lower per case charges, which was derived by reduced per day charge (7.6%) and shorter LOS (1.0%). Rates of readmission also were lower in spine specialty hospitals (OR=0.796). Patient-level and hospital-level factors both played important roles in determining outcome measures.ConclusionsSpine specialty hospitals had higher per day inpatient charges but a much shorter LOS than other types of hospitals due to their specialty volume and experience. In addition, their readmission rate was lower. Spine specialty hospitals also endeavoured to be more efficient after governmental ‘specialty’ designation.
PIK3CA Mutational Status in Circulating Tumor Cells Can Change During Disease Recurrence or Progression in Patients with Breast Cancer
Authors: Markou Athina , Farkona Sofia , Schiza Christina , Efstathiou Tonia , Kounelis Sophia , Malamos Nikos , Georgoulias Vassilis , Lianidou Evi 
Published: November 2014

Abstract
Purpose: Molecular characterization of circulating tumor cells (CTC) is crucial for the investigation of molecular-targeted therapies while PIK3CA somatic mutations play a crucial role in therapy response. We investigated the presence of PIK3CA mutations in CTC and whether this is associated with clinical outcome.
Experimental Design: We developed and validated an ultrasensitive methodology for the detection of PIK3CA mutations that is based on a combination of allele-specific, asymmetric rapid PCR and melting analysis. We analyzed PIK3CA hotspot mutations in: (i) a training group consisting of EpCAM-positive CTC fraction from 37 patients with clinically confirmed metastasis, and 26 healthy female volunteers and 15 primary breast tumor tissues and (ii) an independent group consisting of EpCAM-positive CTC fraction from 57 metastatic and 118 operable breast cancer patients and 76 corresponding primary tumors.
Results: The assay could detect 0.05% of mutated dsDNA in the presence of 99.95% wtDNA for both exons (9 and 20) and was highly specific (0/26 healthy donors). PIK3CA mutations were identified in EpCAM-positive CTC in 20 of 57(35.1%) and in 23 of 118 (19.5%) patients with metastatic and operable breast cancer, and in 45 of 76(59.2%) corresponding FFPEs. Our data indicate that PIK3CA mutational status in CTCs can change during disease progression and is associated with worse survival (P = 0.047).
Conclusions: PIK3CA hotspot mutations are present at a relatively high frequency in CTCs and their presence is associated with worse survival in patients with breast cancer with metastasis. Evaluation of PIK3CA mutational status in CTCs is a strategy with potential clinical application. Clin Cancer Res; 20(22); 5823–34. ©2014 AACR.
Immunotherapy for Primary Brain Tumors: No Longer a Matter of Privilege
Authors: Fecci Peter E. , Heimberger Amy B. , Sampson John H. 
Published: November 2014

AbstractImmunotherapy for cancer continues to gain both momentum and legitimacy as a rational mode of therapy and a vital treatment component in the emerging era of personalized medicine. Gliomas, and their most malignant form, glioblastoma, remain as a particularly devastating solid tumor for which standard treatment options proffer only modest efficacy and target specificity. Immunotherapy would seem a well-suited choice to address such deficiencies given both the modest inherent immunogenicity of gliomas and the strong desire for treatment specificity within the confines of the toxicity-averse normal brain. This review highlights the caveats and challenges to immunotherapy for primary brain tumors, as well as reviewing modalities that are currently used or are undergoing active investigation. Tumor immunosuppressive countermeasures, peculiarities of central nervous system immune access, and opportunities for rational treatment design are discussed.See all articles in this CCR Focus section, “Discoveries, Challenges, and Progress in Primary Brain Tumors.”Clin Cancer Res; 20(22); 5620–9. ©2014 AACR.
Induction of Dendritic Cell–Mediated T-Cell Activation by Modified but Not Native Low-Density Lipoprotein in Humans and Inhibition by Annexin A5
Authors: Liu Anquan , Ming Julia Yue , Fiskesund Roland , Ninio Ewa , Karabina Sonia-Athina , Bergmark Claes , Frostegård Anna G. , Frostegård Johan 
Published: November 2014

Objective—

Atherosclerosis is an inflammatory disease, where activated immunocompetent cells, including dendritic cells (DCs) and T cells are abundant in plaques. Low-density lipoprotein modified either by oxidation (oxLDL) or by human group X-secreted phospholipase A2 (LDLx) and heat shock proteins (HSP), especially HSP60 and 90, have been implicated in atherosclerosis. We previously reported that Annexin A5 inhibits inflammatory effects of phospholipids, decreases vascular inflammation and improves vascular function in apolipoprotein E
−/−
mice. Here, we focus on the LDLx effects on human DCs and T cells.

Approach and Results—
Human DCs were differentiated from peripheral blood monocytes, stimulated by oxLDL or LDLx. Naive autologous T cells were cocultured with pretreated DCs. oxLDL and LDLx, in contrast to LDL, induced DC-activation and T-cell proliferation. T cells exposed to LDLx-treated DCs produced interferon-γ, interleukin (IL)-17 but not IL-4 and IL-10. Annexin A5 abrogated LDLx effects on DCs and T cells and increased production of transforming growth factor-β and IL-10. Furthermore, IL-10 producing T cells suppressed primary T-cell activation via soluble IL-10, transforming growth factor-β, and cell–cell contact. Lentiviral-mediated shRNA knock-down HSP60 and 90 in DCs attenuated the effect of LDLx on DCs and subsequent T-cell proliferation. Experiments on DC and T cells derived from carotid atherosclerotic plaques gave similar results.

Conclusions—
Our data show that modified forms of LDL such as LDLx but not native LDL activate human T cells through DCs. HSP60 and 90 contribute to such T-cell activation. Annexin A5 promotes induction of regulatory T cells and is potentially interesting as a therapeutic agent.
Protein Tyrosine Phosphatase 1B Impairs Diabetic Wound Healing Through Vascular Endothelial Growth Factor Receptor 2 Dephosphorylation
Authors: Zhang Jing , Li Limin , Li Jing , Liu Yuan , Zhang Chen-Yu , Zhang Yujing , Zen Ke 
Published: November 2014

Objective—
Impaired wound healing is a major complication of diabetes mellitus. The mechanisms that govern wound healing, however, are complex and incompletely understood. In the present study, we determined the inhibitory role of protein tyrosine phosphatase 1B (PTP1B) in the process of diabetic wound healing.

Approach and Results—

First, by comparing the wound healing process in PTP1B knockout (PTP1B
−/−
) mice,
ob/ob
mice and their wild-type littermates in the presence or absence of streptozotocin treatment, we showed that the inhibition of mouse wound healing in streptozotocin-induced diabetic conditions is because of the upregulation and activation of PTP1B. Second, the impaired wound healing in
ob/ob
mice and streptozotocin-treated wild-type mice was rescued by a PTP1B inhibitor. Third, PTP1B, which is upregulated under hyperglycemic condition, inhibited the tube formation, proliferation, and migration of human microvascular endothelial cells induced by vascular endothelial growth factor, whereas this inhibition was largely abolished by the PTP1B inhibitor. Finally, mechanism study further indicated that PTP1B likely suppressed the proliferation, migration, and tube formation of vascular endothelial cells through dephosphorylation of vascular endothelial growth factor receptor 2.

Conclusions—
Our study demonstrated that PTP1B negatively modulated the diabetic wound healing process by dephosphorylating the endothelial cell vascular endothelial growth factor receptor 2 and that the specific inhibitor of PTP1B might serve as a potential novel therapeutic tool for diabetic wound healing.
Clinical, Radiological, and Flow-Related Risk Factors for Growth of Untreated, Unruptured Intracranial Aneurysms
Authors: Bor A. Stijntje E. , Tiel Groenestege Andreas T. , terBrugge Karel G. , Agid Ronit , Velthuis Birgitta K. , Rinkel Gabriel J.E. , Wermer Marieke J.H. 
Published: November 2014

Background and Purpose—
Unruptured intracranial aneurysms are frequently followed to monitor aneurysm growth. We studied the yield of follow-up imaging and analyzed risk factors for aneurysm growth.

Methods—
We included patients with untreated, unruptured intracranial aneurysms and ≥6 months of follow-up imaging from 2 large prospectively collected databases. We assessed the proportion of patients with aneurysm growth and performed univariable and multivariable Cox regression analyses to calculate hazard ratios with corresponding 95% confidence intervals (CI) for clinical and radiological risk factors for aneurysm growth. We repeated these analyses for the subset of small (<7 mm) aneurysms.

Results—
Fifty-seven (12%) of 468 aneurysms in 363 patients grew during a median follow-up of 2.1 years (total follow-up, 1372 patient-years). In multivariable analysis, hazard ratios for aneurysm growth were as follows: 1.1 (95% CI, 1.0–1.2) per each additional mm of initial aneurysm size; 2.7 (95% CI, 1.2–6.4) for dome > neck ratio; 2.1 (95% CI, 0.9–4.9) for location in the posterior circulation; and 2.0 (95% CI, 0.8–4.8) for multilobarity. In the subset of aneurysms <7 mm, 37 of 403 (9%) enlarged. In multivariable analysis, hazard ratios for aneurysm growth were 1.1 (95% CI, 0.8–1.5) per each additional mm of initial aneurysm size, 2.2 (95% CI, 1.0–4.8) for smoking, 2.9 (95% CI, 1.0–8.5) for multilobarity, 2.4 (95% CI, 1.0–5.8) for dome/neck ratio, and 2.0 (95% CI, 0.6–7.0) for location in the posterior circulation.

Conclusions—
Initial aneurysm size, dome/neck ratio, and multilobarity are risk factors for aneurysm growth. Cessation of smoking is pivotal because smoking is a modifiable risk factor for growth of small aneurysms.
Functional Outcome After Common Poststroke Complications Occurring in the First 90 Days
Authors: Rohweder Gitta , Ellekjær Hanne , Salvesen Øyvind , Naalsund Eirik , Indredavik Bent 
Published: November 2014

Background and Purpose—
The aim of this study was to explore the associations of common medical complications with functional outcome at 90 days post stroke.

Methods—
Patients with unselected acute stroke were included and observed for 16 predefined complications during the first week. Fifty percent (244 patients) were allocated to follow-up of 13 complications until 90 days and then assessed with the modified Rankin Scale 90. Common complications were defined as complications with frequencies of ≥5%. Ordinal logistic regression (worsened outcome), as well as binary logistic regression for severe dependency and death (modified Rankin Scale score>3) was performed.

Results—

Seven of the 13 complications occurred at a frequency ≥5%. Recurrent stroke and chest infection were found to have an odds ratio for worsened outcome of 7.45 (95% confidence interval, 2.83–20.96;
P
<0.0001) and 3.28 (95% confidence interval, 1.16–9.29;
P
=0.025), respectively. Infections other than chest infections and urinary tract infections had an odds ratio for worsened outcome of 1.59 (95% confidence interval, 1.12–2.24;
P
=0.009) and falls an odds ratio of 1.43 (95% confidence interval, 1.06 to 1.93;
P
=0.021). Myocardial infarction, urinary tract infections, and pain were not associated with a worsened outcome in terms of modified Rankin Scale 90.

Conclusions—
Recurrent stroke and chest infections were strongly associated with a worsened outcome. Other infections and falls were associated with less worsening. For myocardial infarction, urinary tract infections, and pain no association with functional outcome was found. Active strategies for prevention and early treatment of the first 2 complications seem advisable; patient monitoring as part of comprehensive stroke unit care should ensure timely identification and treatment of all complications.