bluuebunny/crossref_metadata_2025_split huggingface dataset:
VEGF-A/NRP1 stimulates GIPC1 and Syx complex formation to promote RhoA activation and proliferation in skin cancer cells
Authors: Yoshida Ayumi , Shimizu Akio , Asano Hirotsugu , Kadonosono Tetsuya , Kondoh Shinae Kizaka , Geretti Elena , Mammoto Akiko , Klagsbrun Michael , Seo Misuzu Kurokawa 
Published: July 2015

ABSTRACTNeuropilin-1 (NRP1) has been identified as a VEGF-A receptor. DJM-1, a human skin cancer cell line, expresses endogenous VEGF-A and NRP1. In the present study, the RNA interference of VEGF-A or NRP1 suppressed DJM-1 cell proliferation. Furthermore, the overexpression of the NRP1 wild type restored shNRP1-treated DJM-1 cell proliferation, whereas NRP1 cytoplasmic deletion mutants did not. A co-immunoprecipitation analysis revealed that VEGF-A induced interactions between NRP1 and GIPC1, a scaffold protein, and complex formation between GIPC1 and Syx, a RhoGEF. The knockdown of GIPC1 or Syx reduced active RhoA and DJM-1 cell proliferation without affecting the MAPK or Akt pathway. C3 exoenzyme or Y27632 inhibited the VEGF-A-induced proliferation of DJM-1 cells. Conversely, the overexpression of the constitutively active form of RhoA restored the proliferation of siVEGF-A-treated DJM-1 cells. Furthermore, the inhibition of VEGF-A/NRP1 signaling upregulated p27, a CDK inhibitor. A cell-penetrating oligopeptide that targeted GIPC1/Syx complex formation inhibited the VEGF-A-induced activation of RhoA and suppressed DJM-1 cell proliferation. In conclusion, this new signaling pathway of VEGF-A/NRP1 induced cancer cell proliferation by forming a GIPC1/Syx complex that activated RhoA to degrade the p27 protein.
The serum concentration of magnesium as a potential state marker in patients with diagnosis of bipolar disorder
Authors: Siwek Marcin , Styczeń Krzysztof , Sowa-Kućma Magdalena , Dudek Dominika , Reczyński Witold , Szewczyk Bernadeta , Misztak Paulina , Opoka Włodzimierz , Topór-Mądry Roman , Nowak Gabriel 
Published: July 2015

CelPojedyncze doniesienia naukowe wskazują na zmiany stężenia magnezu we krwi u osób z chorobą afektywną dwubiegunową (ChAD), jednak dotychczas przeprowadzono bardzo niewiele badań na temat tego zagadnienia. Celem przedstawionego badania było przeprowadzenie oceny stężenia magnezu w surowicy u chorych na ChAD w poszczególnych fazach choroby i w porównaniu do zdrowych ochotnikówMetodaDo badania zakwalifikowano 129 pacjentów z rozpoznaniem ChAD (typ I i II), w tym 58 osób w aktualnym epizodzie depresji, 23 osoby w aktualnym stanie maniakalnym i 48 pacjentów znajdujących się w stanie remisji. Grupa kontrolna liczyła 50 osób. Pomiaru stężenia magnezu dokonano metodą płomieniowej atomowej spektrometrii absorpcyjnej (FAAS)WynikiU pacjentów będących w epizodzie depresyjnym oraz maniakalnym/hipomaniakalnym stężenie magnezu w surowicy było istotnie statystycznie większe w porównaniu z grupą zdrowych ochotników, oraz dodatnio skorelowane z czasem trwania epizodu manii/hipomanii i liczbą zaostrzeń choroby w ostatnim roku. Stężenie magnezu u pacjentów znajdujących się w fazie remisji nie różniło się istotnie od stężenia uzyskanego w grupie kontrolnejWnioskiWyniki badania mogą sugerować rolę stężenia magnezu jako markera stanu, odzwierciedlającego zmiany patofizjologiczne towarzyszące ostrym epizodom CHAD
Inside the Yellow Box: Chang Tsong-Zung (Johnson Chang) in conversation with Lynne Howarth-Gladston and Paul Gladston
Authors: Tsong-Zung Chang , Howarth-Gladston Lynne , Gladston Paul 
Published: July 2015

Abstract
During the early 2000s, Hong Kong-based curator and critic Chang Tsong-Zung (Johnson Chang) developed the concept of the 'Yellow Box' – initially in collaboration with Gao Shiming and Qiu Zhijie – as a critical alternative to the now internationally dominant modes of museum and gallery display known as the White Cube and Black Box. The intention of the Yellow Box is to provide viewing conditions conducive to the showing of contemporary works of art produced using modes of visual expression prevalent traditionally within Chinese cultural contexts, such as those associated with shan shui ink and brush landscape painting. In this conversation Chang reflects critically on implications of the Yellow Box, including its relationship to public space and cultural politics.
Nationalist patriarchy and women painters in Taiwan under the Guomindang
Authors: Ju Jane C 
Published: July 2015

Abstract
The distinctions of works by women artists in Taiwan in recent years are usually linked with influences of feminism and the lifting of martial law in 1987. In truth, women artists in Taiwan have been active since the beginning of the twentieth century and many more became influential after World War II during the period when Madame Chiang Kai-shek and her husband�s Guomindang government directed cultural policies. The efforts of these early artists have been neglected in art historical studies because their works were mostly traditional and not innovative. This article will re-evaluate the meaning of these artists in Taiwan art history from alternate perspectives of modernity, namely, the importance of traditionalism in modern art and the role of patriarchal nationalism in defining a national art form.
Walking at the extreme edge of rationality: Yu Haibo in Conversation with Yu Tianqi Kiki
Authors: Yu Haibo , Yu Tianqi Kiki 
Published: July 2015

Abstract
Yu Haibo is one of the first photographers in the People�s Republic of China to be directly influenced by Surrealist thinking and practice after the ending of the Cultural Revolution. In this conversation with his daughter Yu Tianqi Kiki, Yu reflects on his development as a photographer and the relationship of his work to the western philosophical tradition and the shifting socio-economic and political context in the PRC since the early 1980s.
Lowered cutoff of lymph node fine-needle aspiration thyroglobulin in thyroid cancer patients with serum anti-thyroglobulin antibody
Authors: Jo Kwanhoon , Kim Min-Hee , Lim Yejee , Jung So-Lyung , Bae Ja-Seong , Jung Chan-Kwon , Kang Moo-Il , Cha Bong-Yun , Lim Dong-Jun 
Published: July 2015

ObjectiveFine needle aspiration cytology (FNAC) and measurement of thyroglobulin (Tg) in needle washout (FNA-Tg) are recommended for the diagnosis of metastatic or recurrent lymph nodes (LNs) in differentiated thyroid cancer (DTC). However, the effect of serum Tg antibody (TgAb) on FNA-Tg levels still remains unclear in the preoperative setting. We analyze the interference of serum TgAb on FNA-Tg levels as proof of concept in the diagnostic advantage of serum TgAb combined with FNA-Tg.Subjects and methodsA total of 370 suspicious cervical LNs from 273 patients with DTC were included. The primary tumor was confirmed as DTC on preoperative pathology in all patients. We performed FNA-Tg measurement and FNAC on suspicious LNs and evaluated the diagnostic performance of FNAC and FNA-Tg according to TgAb status. Final diagnoses were confirmed by histological examination of excised specimens or by follow-up ultrasonography for at least 6 months.ResultsData from 273 subjects with suspicious 370 LNs were evaluated. Fifty-five LNs (14.9%) were from TgAb+ positive serum TgAb (TgAb+) patients. Serum Tg and FNA-Tg levels were significantly lower in patients with TgAb+ than in those with TgAb-negative (TgAb−). Final pathology confirmed 109 LNs (29.5%) asmalignant. Diagnostic performance of FNA-Tg at the same cutoff level was lower in the TgAb+ than TgAb− group. FNA-Tg cutoff levels determined by ROC curve were lower in the TgAb+ group.ConclusionThe results suggested that the cutoff value of FNA-Tg should be lowered in suspicious LN before thyroidectomy in thyroid cancer patients with TgAb.
Beyond 2/3 and 1/3: The Complex Signatures of Sex-Biased Admixture on the X Chromosome
Authors: Goldberg Amy , Rosenberg Noah A 
Published: July 2015

Abstract
Sex-biased demography, in which parameters governing migration and population size differ between females and males, has been studied through comparisons of X chromosomes, which are inherited sex-specifically, and autosomes, which are not. A common form of sex bias in humans is sex-biased admixture, in which at least one of the source populations differs in its proportions of females and males contributing to an admixed population. Studies of sex-biased admixture often examine the mean ancestry for markers on the X chromosome in relation to the autosomes. A simple framework noting that in a population with equally many females and males, two-thirds of X chromosomes appear in females, suggests that the mean X-chromosomal admixture fraction is a linear combination of female and male admixture parameters, with coefficients 2/3 and 1/3, respectively. Extending a mechanistic admixture model to accommodate the X chromosome, we demonstrate that this prediction is not generally true in admixture models, although it holds in the limit for an admixture process occurring as a single event. For a model with constant ongoing admixture, we determine the mean X-chromosomal admixture, comparing admixture on female and male X chromosomes to corresponding autosomal values. Surprisingly, in reanalyzing African-American genetic data to estimate sex-specific contributions from African and European sources, we find that the range of contributions compatible with the excess African ancestry on the X chromosome compared to autosomes has a wide spread, permitting scenarios either without male-biased contributions from Europe or without female-biased contributions from Africa.
Comparison of different methods of antioxidant activity evaluation of green and roast C. Arabica and C. Robusta coffee beans
Authors: Pokorná J. , Venskutonis P. R. , Kraujalyte V. , Kraujalis P. , Dvořák P. , Tremlová B. , Kopřiva V. , Ošťádalová M. 
Published: July 2015

Coffee beans contain a large amount of antioxidants, which are subjected to various changes during roasting. In this study, antioxidant potential of raw and roasted to different degree (light, medium, dark) C. arabica and C. robusta coffee beans was evaluated by the four antioxidant assay methods, TPC, FRAP, TEAC, and DPPH˙.
The obtained results revealed significant differences between the coffee types, roasting degree, and antioxidant activity assessment methods. FRAP and TPC appeared to be the most appropriate methods for revealing the differences in antioxidant potential of different coffee types and the effects of roasting. The results obtained by these methods were in good correlation. ABTS and DPPH? methods are not enough sensitive for the determination of roasting degrees.
In general, based on statistical data evaluation, antioxidant activity is more dependent on the coffee type than on the degree of roasting, however, the selection of analytical method may also be significant.
Rancière: pedagogy and politics
Authors: Harris David Ernest 
Published: July 2015

<p>Rancière’s work on education is becoming widely known, but it must be understood in its context to avoid any misleadingly conventional readings and to grasp its general importance. The work on industrial history is obviously connected, but so are the more technical academic criticisms of Althusser, Bourdieu and Marx. These add considerably to conventional discussion by identifying a crucial contradiction between emancipatory goals and necessary hierarchies based on expertise.</p><p> </p><p>Rancière’s work on aesthetics as a democratic arena has inspired some recent educational experiments in participation. His historical research can also be seen as providing support for current educational struggles against neoliberalism.</p><p> </p><p>Rancière’s methods are assessed critically in turn, and the connections with Foucault can be seen to both unify the work overall and raise difficulties of its own.</p>
On the Properties of the Priority Deriving Procedure in the Pairwise Comparisons Method
Authors: Kułakowski Konrad 
Published: July 2015

The pairwise comparisons method can be used when the relative order of preferences among different concepts (alternatives) needs to be determined. There are several popular implementations of this method, including the Eigenvector Method, the Least Squares Method, the Chi Squares Method and others. Each of the above methods comes with one or more inconsistency indices that help to decide whether the consistency of input guarantees obtaining a reliable output, thus taking the optimal decision. This article explores the relationship between inconsistency of input and error of output. An error describes to what extent the obtained results correspond to the single expert’s assessments. On the basis of the inconsistency and the error, two properties of the weight deriving procedure are formulated. These properties are proven for eigenvector method and Koczkodaj’s inconsistency index. Several estimates using Koczkodaj’s inconsistency index for a principal eigenvalue, Saaty’s inconsistency index and the Condition of Order Preservation are also provided.
Au-ACRAMTU-PEt3 Alters Redox Balance To Inhibit T Cell Proliferation and Function
Authors: Langston P. Kent , Yang Mu , Bierbach Ulrich , Parsonage Derek , Poole Leslie B. , Price Madeline J. , Grayson Jason M. 
Published: July 2015

Abstract
Although T cells play a critical role in protection from viruses, bacteria, and tumors, they also cause autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis, and multiple sclerosis. Unwanted T cell responses during organ transplant, graft-versus-host disease, and allergies are also major clinical problems. Although drugs are available to suppress unwanted immune responses, they have limited efficacy with serious side effects. Thus, new therapeutics limiting T cell activation, proliferation, and function can make an immediate clinical impact. To identify new suppressors of lymphocyte activation, proliferation, and function, we examined the immunosuppressive activity of gold(I) analogs of platinum-acridine antitumor agents. We found that the gold complex Au-ACRAMTU-PEt3 is a potent suppressor of murine and human T cell activation. Preincubation with Au-ACRAMTU-PEt3 suppresses the proliferation of CD4+ and CD8+ T cells at a similar concentration as pharmaceutical grade cyclosporine A. Au-ACRAMTU-PEt3 pretreatment decreases the production of IFN-γ, TNF-α, IL-2, and IL-17 by human and murine CD4+ and CD8+ T cells. When mice were treated with Au-ACRAMTU-PEt3 during viral infection, the expansion of virus-specific CD8+ T cells was decreased 10-fold and viral load was elevated. Taken together, these results demonstrate that Au-ACRAMTU-PEt3 has potent immunosuppressive activity that could be used to suppress immune responses during transplantation and autoimmunity.
Nucleotide-Induced Membrane-Proximal Proteolysis Controls the Substrate Specificity of T Cell Ecto–ADP-Ribosyltransferase ARTC2.2
Authors: Menzel Stephan , Rissiek Björn , Bannas Peter , Jakoby Thomas , Miksiewicz Maria , Schwarz Nicole , Nissen Marion , Haag Friedrich , Tholey Andreas , Koch-Nolte Friedrich 
Published: July 2015

Abstract
ARTC2.2 is a toxin-related, GPI-anchored ADP-ribosyltransferase expressed by murine T cells. In response to NAD+ released from damaged cells during inflammation, ARTC2.2 ADP-ribosylates and thereby gates the P2X7 ion channel. This induces ectodomain shedding of metalloprotease-sensitive cell surface proteins. In this study, we show that ARTC2.2 itself is a target for P2X7-triggered ectodomain shedding. We identify the metalloprotease cleavage site 3 aa upstream of the predicted GPI anchor attachment site of ARTC2.2. Intravenous injection of NAD+ increased the level of enzymatically active ARTC2.2 in serum, indicating that this mechanism is operative also under inflammatory conditions in vivo. Radio–ADP-ribosylation assays reveal that shedding refocuses the target specificity of ARTC2.2 from membrane proteins to secretory proteins. Our results uncover nucleotide-induced membrane-proximal proteolysis as a regulatory mechanism to control the substrate specificity of ARTC2.2.
The T Cell Repertoire–Diversifying Enzyme TSSP Contributes to Thymic Selection of Diabetogenic CD4 T Cell Specificities Reactive to ChgA and IAPP Autoantigens
Authors: Viret Christophe , Mahiddine Karim , Baker Rocky Lee , Haskins Kathryn , Guerder Sylvie 
Published: July 2015

Abstract
Multiple studies highlighted the overtly self-reactive T cell repertoire in the diabetes-prone NOD mouse. This autoreactivity has primarily been linked to defects in apoptosis induction during central tolerance. Previous studies suggested that thymus-specific serine protease (TSSP), a putative serine protease expressed by cortical thymic epithelial cells and thymic dendritic cells, may edit the repertoire of self-peptides presented by MHC class II molecules and shapes the self-reactive CD4 T cell repertoire. To gain further insight into the role of TSSP in the selection of self-reactive CD4 T cells by endogenous self-Ags, we examined the development of thymocytes expressing distinct diabetogenic TCRs sharing common specificity in a thymic environment lacking TSSP. Using mixed bone marrow chimeras, we evaluated the effect of TSSP deficiency confined to different thymic stromal cells on the differentiation of thymocytes expressing the chromogranin A–reactive BDC-2.5 and BDC-10.1 TCRs or the islet amyloid polypeptide–reactive TCR BDC-6.9 and BDC-5.2.9. We found that TSSP deficiency resulted in deficient positive selection and induced deletion of the BDC-6.9 and BDC-10.1 TCRs, but it did not affect the differentiation of the BDC-2.5 and BDC-5.2.9 TCRs. Hence, TSSP has a subtle role in the generation of self-peptide ligands directing diabetogenic CD4 T cell development. These results provide additional evidence for TSSP activity as a novel mechanism promoting autoreactive CD4 T cell development/accumulation in the NOD mouse.
Reshaping of Human Macrophage Polarization through Modulation of Glucose Catabolic Pathways
Authors: Izquierdo Elena , Cuevas Víctor Delgado , Fernández-Arroyo Salvador , Riera-Borrull Marta , Orta-Zavalza Emmanuel , Joven Jorge , Rial Eduardo , Corbi Angel L. , Escribese María M. 
Published: July 2015

Abstract
Macrophages integrate information from the tissue microenvironment and adjust their effector functions according to the prevalent extracellular stimuli. Therefore, macrophages can acquire a variety of activation (polarization) states, and this functional plasticity allows the adequate initiation, regulation, and resolution of inflammatory responses. Modulation of the glucose metabolism contributes to the macrophage adaptation to the surrounding cytokine milieu, as exemplified by the distinct glucose catabolism of macrophages exposed to LPS/IFN-γ or IL-4. To dissect the acquisition of macrophage effector functions in the absence of activating cytokines, we assessed the bioenergetic profile of macrophages generated in the presence of GM-CSF (GM-MØ) or M-CSF (M-MØ), which do not release pro- or anti-inflammatory cytokines unless subjected to additional activating stimuli. Compared to M-MØ, GM-MØ displayed higher oxygen consumption rate and aerobic glycolysis (extracellular acidification rate [ECAR]), as well as higher expression of genes encoding glycolytic enzymes. However, M-MØ exhibited a significantly higher oxygen consumption rate/ECAR ratio. Surprisingly, whereas aerobic glycolysis positively regulated IL1B, TNF, and INHBA mRNA expression in both macrophage subtypes, mitochondrial respiration negatively affected IL6, IL1B, TNF, and CXCL10 mRNA expression in M-MØ. The physiological significance of these results became evident under low oxygen tensions, as hypoxia enhanced ECAR in M-MØ via HIF-1α and HIF-2α, increased expression of glycolytic enzymes and GM-MØ–specific genes, and diminished expression of M-MØ–associated genes. Therefore, our data indicate that GM-MØ and M-MØ display distinct bioenergetic profiles, and that hypoxia triggers a transcriptomic switch in macrophages by promoting a HIF-1α/HIF-2α-dependent increase in ECAR.
Genetic Interaction between Lyn, Ets1, and Btk in the Control of Antibody Levels
Authors: Mayeux Jessica , Skaug Brian , Luo Wei , Russell Lisa M. , John Shinu , Saelee Prontip , Abbasi Hansaa , Li Quan-Zhen , Garrett-Sinha Lee Ann , Satterthwaite Anne B. 
Published: July 2015

AbstractTight control of B cell differentiation into plasma cells (PCs) is critical for proper immune responses and the prevention of autoimmunity. The Ets1 transcription factor acts in B cells to prevent PC differentiation. Ets1−/− mice accumulate PCs and produce autoantibodies. Ets1 expression is downregulated upon B cell activation through the BCR and TLRs and is maintained by the inhibitory signaling pathway mediated by Lyn, CD22 and SiglecG, and SHP-1. In the absence of these inhibitory components, Ets1 levels are reduced in B cells in a Btk-dependent manner. This leads to increased PCs, autoantibodies, and an autoimmune phenotype similar to that of Ets1−/− mice. Defects in inhibitory signaling molecules, including Lyn and Ets1, are associated with human lupus, although the effects are more subtle than the complete deficiency that occurs in knockout mice. In this study, we explore the effect of partial disruption of the Lyn/Ets1 pathway on B cell tolerance and find that Lyn+/−Ets1+/− mice demonstrate greater and earlier production of IgM, but not IgG, autoantibodies compared with Lyn+/− or Ets1+/− mice. We also show that Btk-dependent downregulation of Ets1 is important for normal PC homeostasis when inhibitory signaling is intact. Ets1 deficiency restores the decrease in steady state PCs and Ab levels observed in Btk−/− mice. Thus, depending on the balance of activating and inhibitory signals to Ets1, there is a continuum of effects on autoantibody production and PC maintenance. This ranges from full-blown autoimmunity with complete loss of Ets1-maintaining signals to reduced PC and Ab levels with impaired Ets1 downregulation.
The TNF Family Molecules LIGHT and Lymphotoxin αβ Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells
Authors: da Silva Antunes Ricardo , Madge Lisa , Soroosh Pejman , Tocker Joel , Croft Michael 
Published: July 2015

Abstract
Lung epithelial cells are considered important sources of inflammatory molecules and extracellular matrix proteins that contribute to diseases such as asthma. Understanding the factors that stimulate epithelial cells may lead to new insights into controlling lung inflammation. This study sought to investigate the responsiveness of human lung epithelial cells to the TNF family molecules LIGHT and lymphotoxin αβ (LTαβ). Bronchial and alveolar epithelial cell lines, and primary human bronchial epithelial cells, were stimulated with LIGHT and LTαβ, and expression of inflammatory cytokines and chemokines and markers of epithelial-mesenchymal transition and fibrosis/remodeling was measured. LTβ receptor, the receptor shared by LIGHT and LTαβ, was constitutively expressed on all epithelial cells. Correspondingly, LIGHT and LTαβ strongly induced a limited but highly distinct set of inflammatory genes in all epithelial cells tested, namely the adhesion molecules ICAM-1 and VCAM-1; the chemokines CCL5, CCL20, CXCL1, CXCL3, CXCL5, and CXCL11; the cytokines IL-6, activin A and GM-CSF; and metalloproteinases matrix metalloproteinase-9 and a disintegrin and metalloproteinase domain-8. Importantly, induction of the majority of these inflammatory molecules was insensitive to the suppressive effects of the corticosteroid budesonide. LIGHT and LTαβ also moderately downregulated E-cadherin, a protein associated with maintaining epithelial integrity, but did not significantly drive production of extracellular matrix proteins or α-smooth muscle actin. Thus, LIGHT and LTαβ induce a distinct steroid-resistant inflammatory signature in airway epithelial cells via constitutively expressed LTβ receptor. These findings support our prior murine studies that suggested the receptors for LIGHT and LTαβ contribute to development of lung inflammation characteristic of asthma and idiopathic pulmonary fibrosis.