bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of new phosphorylation sites of AMPA receptors in the rat hippocampus—A resource for neuroscience research
Authors: Ghafari Maryam , Keihan Falsafi Soheil , Höger Harald , Bennett Keiryn L. , Lubec Gert 
Published: February 2015

PurposeAMPA (alpha‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazole propionic acid) receptors (AMPARs) are glutamate‐gated ion channels that mediate the majority of fast excitatory synaptic transmissions in the mammalian brain. A series of phosphorylation sites have been predicted or identified and knowledge on phosphorylations is mandatory for understanding receptor biology and functions.Experimental designImmunoprecipitation from extracted hippocampal rat proteins was carried out using an antibody against the AMPAR GluA1 subunit, followed by identification of GluA1 and binding partners by MS. Bands from SDS‐PAGE were picked, peptides were generated by trypsin and chymotrypsin digestion and identified by MS/MS (LTQ Orbitrap Velos).ResultsUsing Mascot as a search engine, phosphorylation sites S506, S645, S720, S849, S863, S895, T858, Y228, Y419, and T734 were found on GluA1; S357, S513, S656, S727, T243, T420, T741, Y 143, Y301,Y426 on GluA2; S301, S516, S657, S732, T222, and T746 were observed on GluA3; and S514, S653 was phosphorylated on GluA4.Conclusions and clinical relevanceA series of additional protein modifications were observed and in particular, tyrosine and tryptophan nitrations on GluA1 were detected that may raise questions on additional regulation mechanisms for AMPARs in addition to phosphorylations. The findings are relevant for interpretation of previous work and design of future studies using AMPAR serving as a resource for neuroscience research and indeed, phosphorylations and PTMs per se would have to be respected when neuropathological and neurological disorders are being studied.
Position‐based adaptive quantization for target location estimation in wireless sensor networks using one‐bit data
Authors: Liu Guiyun , Liu Hua , Chen Hongbin , Zhou Chao , Shu Lei 
Published: February 2015

AbstractThe problem of target location estimation in a wireless sensor network is considered, where due to the bandwidth and power constraints, each sensor only transmits one‐bit information to its fusion center. To improve the performance of estimation, a position‐based adaptive quantization scheme for target location estimation in wireless sensor networks is proposed to make a good choice of quantizer' thresholds. By the proposed scheme, each sensor node dynamically adjusts its quantization threshold according to a kind of position‐based information sequences and then sends its one‐bit quantized version of the original observation to a fusion center. The signal intensity received at local sensors is modeled as an isotropic signal intensity attenuation model. The position‐based maximum likelihood estimator as well as its corresponding position‐based Cramér–Rao lower bound are derived. Numerical results show that the position‐based maximum likelihood estimator is more accurate than the classical fixed‐quantization maximum likelihood estimator and the position‐based Cramér–Rao lower bound is less than its fixed‐quantization Cramér‐Rao lower bound. Copyright © 2015 John Wiley & Sons, Ltd.
Exploring hypertension genome‐wide association studies findings and impact on pathophysiology, pathways, and pharmacogenetics
Authors: Cabrera Claudia P , Ng Fu Liang , Warren Helen R , Barnes Michael R , Munroe Patricia B , Caulfield Mark J 
Published: February 2015

Hypertension is a major risk factor for global mortality. Recent genome‐wide association studies (GWAS) have led to successful identification of many genetic loci influencing blood pressure, although these studies account for less than 5% of heritability. While genetic discovery efforts continue, it is timely to pause and reflect on what information has been gained to date from reported loci. Knowledge from GWAS findings inform our understanding of the pathways and pleiotropy underpinning hypertension and aid in the identification of potential druggable targets. By reviewing blood pressure loci we aim to determine how much potential the current observations have for future clinical utility. WIREs Syst Biol Med 2015, 7:73–90. doi: 10.1002/wsbm.1290This article is categorized under:

Translational, Genomic, and Systems Medicine > Translational Medicine
Synthesis and Photoluminescence Properties of the Red‐Emitting Phosphor Mg3(BN2)N Doped with Eu2+
Authors: Schölch Jessica , Dierkes Tobias , Enseling David , Ströbele Markus , Jüstel Thomas , Meyer H.‐Jürgen 
Published: February 2015

Abstract Mg3(BN2)N was prepared by solid state metathesis reactions and several europium (Eu2+) doped samples were prepared to discover novel red‐emitting photoluminescent (PL) materials. It turned out that the undoped and doped samples showed very broad deep‐red photoluminescence ranging from about 500 nm into the near infrared. Due to the similar spectra of the undoped and doped samples and unusually high FWHM values of about 5780 cm–1 we conclude that the luminescence process originates from defect sites. This was confirmed by decay measurements which show that the decay constants for all samples were in the range of several milliseconds.
Reactivity with Amines of Bis(cyanamide) and Bis(cyanoguanidine) Complexes of the Iron Triad
Authors: Albertin Gabriele , Antoniutti Stefano , Caia Alfonso , Castro Jesús 
Published: February 2015

Abstract Treatment of bis(cyanamide) [M(N≡CNEt2)2L4](BPh4)2 and bis(cyanoguanidine) [M{N≡CN(H)C(NH2)=NH}2L4](BPh4)2 complexes [M = Fe, Ru, Os; L = P(OEt)3] with an excess of amine RNH2 (R = nPr, iPr) affords mixed‐ligand complexes with cyanamide and amine [M(NH2R)(N≡CNEt2)L4](BPh4)2 (1a–5a) and [M(NH2R){N≡CN(H)C(NH2)=NH}L4](BPh4)2 (1b, 2b). The complexes were characterized by spectroscopy and X‐ray crystal structure determination of [M(NH2iPr)(N≡CNEt2){P(OEt)3}4](BPh4)2 [M = Ru (3a), Os (5a)].
Deficiencies in the Availability of Essential Musculoskeletal Surgical Services at 883 Health Facilities in 24 Low‐ and Lower‐Middle‐Income Countries
Authors: Spiegel D. A. , Nduaguba A. , Cherian M. N. , Monono M. , Kelley E. T. 
Published: February 2015

AbstractBackgroundThe sequelae of acute musculoskeletal conditions, especially injuries and infections, are responsible for significant disability in low‐ and middle‐income countries. This study characterizes the availability of selected musculoskeletal surgical services at different tiers of the health system in a convenience sample of 883 health facilities from 24 low‐ and lower‐middle‐income countries.MethodsSelected data points from the World Health Organization’s (WHO) tool of situational analysis of surgical availability were extracted from the WHO’s database in December, 2013. These included infrastructure, physical resources and supplies, interventions, and human resources. For a descriptive analysis, facilities were divided into two groups based on number of beds (<100, 100–300, and >300) and level of facility (primary referral, secondary/tertiary, and Private/NGO/Mission). Statistical comparison was made between public and Private/NGO/Mission facilities based on number of beds (≤100, 100–300, and >300) using a Chi‐Square analysis, with statistical significance at p < 0.05.FindingsSignificant deficiencies were noted in infrastructure, physical resources and supplies, and human resources for the provision of essential orthopedic surgical services at all tiers of the health system. Availability was significantly lower in public versus Private/NGO/Mission facilities for nearly all categories in facilities with ≤100 beds, and in a subset of measures in facilities with between 100 and 300 beds.InterpretationDeficiencies in the availability of orthopedic surgical services were observed at all levels of health facility and were most pronounced at facilities with ≤100 beds in the public sector. Strengthening the delivery of essential surgical services, including orthopedics, at the primary referral level must be prioritized if we are to reduce the burden of death and disability from a variety of emergent health conditions.FundingThere were no sources of funding.
Impact of Combination Criteria of Nodal Counts and Sizes on Preoperative MDCT in Advanced Gastric Cancer
Authors: Kawaguchi Tsutomu , Ichikawa Daisuke , Komatsu Shuhei , Kosuga Toshiyuki , Kubota Takeshi , Okamoto Kazuma , Shiozaki Atsushi , Fujiwara Hitoshi , Konishi Hirotaka , Morimura Ryo , Murayama Yasutoshi , Kuriu Yoshiaki , Ikoma Hisashi , Nakanishi Masayoshi , Sakakura Chouhei , Otsuji Eigo 
Published: February 2015

AbstractBackgroundThis study was designed to establish a new diagnostic criteria of preoperative nodal status with high specificity on multidetector row computed tomography (MDCT) to extract advanced gastric cancer (GC) patients with an unfavorable prognosis.MethodFrom total of 480 GC patients who underwent surgical resection, 58 consecutive patients with a preoperative diagnosis of nodal metastasis on MDCT were included in this retrospective study. Nodal status on MDCT was reassessed and a prognostic analysis was performed in the present study.ResultsAmong several clinical nodal diagnostic criteria on MDCT, a cut‐off value of the short‐axis diameter measuring ≥15 mm showed the highest specificity (100 %). The prognostic analysis revealed that patients with nodal counts on MDCT of ≥3 (p = 0.035) as well as those with a nodal diameter ≥15 mm (p < 0.001) had a significantly poor prognosis. When patients with nodal counts ≥3 and at least one node that was ≥15 mm in diameter on MDCT were defined as having extensive lymph node metastasis (ELM) that had a significantly poor prognosis (5‐year survival rates of 20 %, p < 0.001), and ELM was also an independent poor prognostic factor in advanced GC (p = 0.033, hazard ratio 4.038).ConclusionGC patients with nodal counts ≥3 and at least one node that was ≥15 mm in diameter on MDCT have extremely worse prognose with high diagnostic specificity. This novel surrogate indicator for preoperative imaging diagnosis may help personalized therapeutic strategies for GC patients with ELM.
Obstructive sleep apnoea syndrome does not negatively affect oral and dental health
Authors: Acar M , Türkcan İ , Özdaş T , Bal C , Cingi C 
Published: February 2015

AbstractObjective:Obstructive sleep apnoea syndrome can lead to unhealthy open-mouth breathing. We investigated the possible relationship between obstructive sleep apnoea syndrome and dental health. We also evaluated other clinical factors that may affect oral health.Methods:We measured sleep using polysomnography and determined the apnoea–hypopnoea index for a total of 291 patients. We also recorded the demographic data, duration of snoring complaints, educational status and income levels for our patient cohort; finally, we calculated the decayed, missing and filled teeth index.Results:Forty-one patients presented with primary snoring (control group) and 250 patients (study group) presented with mild, moderate and severe obstructive sleep apnoea syndrome. We found no correlation between obstructive sleep apnoea syndrome severity and the decayed, missing and filled teeth index (p = 0.057). We also found no correlation between the apnoea–hypopnoea and decayed, missing and filled teeth indexes. Age and the duration of snoring complaints were positively correlated with the decayed, missing and filled teeth index while educational status and income levels were negatively correlated (p < 0.001).Conclusion:Obstructive sleep apnoea syndrome does not negatively affect oral and dental health.
Long-term effects of intratympanic methylprednisolone perfusion treatment on intractable Ménière's disease
Authors: She W , Lv L , Du X , Li H , Dai Y , Lu L , Ma X , Chen F 
Published: February 2015

AbstractObjective:This study aimed to investigate the long-term efficacy of intratympanic methylprednisolone perfusion treatment for intractable Ménière's disease.Methods:A retrospective analysis of 17 intractable Ménière's disease patients treated with intratympanic methylprednisolone perfusion was performed. Treatment efficacy was evaluated according to the American Academy of Otolaryngology–Head and Neck Surgery criteria. Short and long-term control or improvement rates were calculated after 6 and 24 months, respectively.Results:Sixteen patients were followed for more than two years. Short- and long-term vertigo control rates were 94 per cent and 81 per cent, respectively; short- and long-term functional activity improvements were 94 per cent and 88 per cent, respectively. The pure tone average was 53 ± 14 dB before treatment, and 50 ± 16 dB at 6 months and 52 ± 20 dB at 24 months after intratympanic methylprednisolone perfusion. Tinnitus was controlled or improved in five patients over the two-year follow-up period.Conclusion:Intratympanic methylprednisolone perfusion can effectively control vertigo and improve functional activity in intractable Ménière's disease patients with good hearing preservation. It may therefore be a viable alternative treatment for intractable Ménière's disease.
Identifying challenges to enforcement in protected areas: empirical insights from 15 Colombian parks
Authors: Nolte Christoph 
Published: February 2015

AbstractProtected areas are intended to conserve biodiversity by restricting human activities within their boundaries. However, such restrictions are difficult to enforce fully in many tropical parks. Improving regulatory enforcement requires an understanding of prevailing challenges to detection and sanctioning activities. Drawing from empirical field research in 15 Colombian parks, I show that current enforcement efforts may be insufficient to deter most priority threats. For long-term infractions, such as agriculture, livestock grazing, and construction, sanctioning violators is challenging, whereas for furtive infractions, such as logging and hunting, it may be difficult to detect violators. Investment in staff, equipment and infrastructure may fail to increase enforcement capacity and yield positive conservation outcomes unless accompanied by resolution of land tenure, clarification of use rights, improved patrolling strategies and protection of park guards.
CONRAD AND THE COMIC TURN
Authors: Kerr Douglas 
Published: February 2015

Of his nineteen years as a sailor, from 1874 to 1894, Joseph Conrad actually worked on ships for ten years and eight months, of which just over eight years were spent at sea, including nine months as a passenger (Najder 161–62). During these nomadic years, London was the place to which he returned again and again to seek his next berth, staying in a series of sailors’ homes, lodgings, and boarding houses. How did he spend his time, a single man with no family and few friends, whose main occupation was waiting? He recalled, in the preface toThe Secret Agent, “solitary and nocturnal walks all over London in my early days” (7). Ford Madox Ford says that Conrad knew all the bars around Fenchurch Street (which links the financial centre of the City of London to Whitechapel and the East End) from his days of waiting for a ship. Returning to the area later in life, according to Ford's slightly improbable memory, he “became at once the city-man gentleman-adventurer with an eye for a skirt,” who “could tell you where every husky earringed fellow with a blue, white-spotted handkerchief under his arm was going to. . . .” (Joseph Conrad116, 117). The reality of these London sojourns was probably less romantic, most of the time. But there was one place where a sailor ashore, without much money, could always go for company and entertainment: the music-hall.
A preliminary study to develop an intervention to facilitate communication between couples in advanced cancer
Authors: Mowll Jane , Lobb Elizabeth A. , Lane Lisbeth , Lacey Judith , Chochinov Harvey M. , Kelly Brian , Agar Meera , Links Matthew , Kearsley John H. 
Published: February 2015

AbstractObjective:Psychosocial interventions directed to couples where one has advanced cancer can reduce distress, enhance communication, and provide an opportunity for relational growth. The present study aimed to develop an intervention to facilitate communication about living with advanced cancer using the Patient Dignity Inventory (PDI) as the focus of a clinical interview with couples toward the end of life.Method:Couples were recruited from oncology and palliative care services at a Sydney hospital. After the PDI was developed and manualized as an intervention for couples, the PDI–Couple Interview (PDI–CI) was delivered by a clinical psychologist and comprised the following: (1) the patient completed the PDI; (2) the patient's identified partner completed the PDI about how they thought the patient was feeling; and (3) the clinician reviewed the results with the couple, summarizing areas of concurrence and discordance and facilitating discussion.Results:Some 34 couples were referred, of which 12 consented, 9 of whom completed the clinical interview. Reported benefits included enabling couples to express their concerns together, identifying differences in understanding, and giving “permission to speak” with each other. The focus of the interview around the PDI provided a structure that was particularly acceptable for men. Most couples confirmed that they were “on the same page,” and where differences were identified, it provided a forum for discussion and a mutual understanding of the challenges in managing advanced cancer within a supportive context.Significance of Results:Participant couples' experiences of the PDI–CI provide valuable insight into the benefits of this intervention. This preliminary study indicates that the intervention is a relatively simple means of enhancing closer communication and connection between couples where one has advanced cancer and may be an important adjunct in helping prepare couples for the challenges inherent toward the end of life. Further investigation of feasibility with a larger sample is recommended.
Performance management: the clinician’s tale
Authors: Smith Peter C. 
Published: February 2015

AbstractThe success of performance management in health care depends crucially on the response of health care professionals to the various instruments of performance management (PM). Yet we know little about how those professionals think and work. They may ignore PM for three reasons: ignorance of the relevant performance information; lack of faith in the credibility of the information; or inability to take relevant action. Furthermore, there is a risk that PM comes into fundamental conflict with the idea of professional autonomy and personalization of care. In order to create more effective PM policies, we need to know much more about professional motivations and constraints.
A common genetic variation of melanoma inhibitory activity-2 labels a subtype of pancreatic adenocarcinoma with high endoplasmic reticulum stress levels
Authors: Kong Bo , Wu Weiwei , Valkovska Nataliya , Jäger Carsten , Hong Xin , Nitsche Ulrich , Friess Helmut , Esposito Irene , Erkan Mert , Kleeff Jörg , Michalski Christoph W. 
Published: February 2015

AbstractHNF1 homeobox A (HNF1A)-mediated gene expression constitutes an essential component of the secretory pathway in the exocrine pancreas. Melanoma inhibitory activity 2 (MIA2), a protein facilitating protein secretion, is an HNF1A target. Protein secretion is precisely coordinated by the endoplasmic reticulum (ER) stress/unfolded protein response (UPR) system. Here, we demonstrate that HNFA and MIA2 are expressed in a subset of human PDAC tissues and that HNF1A induced MIA2 in vitro. We identified a common germline variant of MIA2 (c.A617G: p.I141M) associated with a secretory defect of the MIA2 protein in PDAC cells. Patients carrying MIA2I141M survived longer after tumor resection but the survival benefit was restricted to those patients who received adjuvant chemotherapy. The MIA2I141M variant was associated with high expression of ER stress/UPR genes – in particular those of the ERN1/XBP arm – in human PDAC samples. Accordingly, PDAC cell lines expressing the MIA2I141M variant expressed high levels of ERN1 and were more sensitive to gemcitabine. These findings define an interaction between the common MIA2I141M variant and the ER stress/UPR system and specify a subgroup of PDAC patients who are more likely to benefit from adjuvant chemotherapy.
Contrasting responses of Central Asian rock glaciers to global warming
Authors: Sorg Annina , Kääb Andreas , Roesch Andrea , Bigler Christof , Stoffel Markus 
Published: February 2015

AbstractWhile the responses of Tien Shan glaciers – and glaciers elsewhere – to climatic changes are becoming increasingly well understood, this is less the case for permafrost in general and for rock glaciers in particular. We use a novel approach to describe the climate sensitivity of rock glaciers and to reconstruct periods of high and low rock glacier activity in the Tien Shan since 1895. Using more than 1500 growth anomalies from 280 trees growing on rock glacier bodies, repeat aerial photography from Soviet archives and high-resolution satellite imagery, we present here the world's longest record of rock glacier movements. We also demonstrate that the rock glaciers exhibit synchronous periods of activity at decadal timescales. Despite the complex energy-balance processes on rock glaciers, periods of enhanced activity coincide with warm summers and the annual mass balance of Tuyuksu glacier fluctuates asynchronously with rock glacier activity. At multi-decadal timescales, however, the investigated rock glaciers exhibit site-specific trends reflecting different stages of inactivation, seemingly in response to the strong increase in air temperature since the 1970s.
System-wide analysis of the transcriptional network of human myelomonocytic leukemia cells predicts attractor structure and phorbol-ester-induced differentiation and dedifferentiation transitions
Authors: Sakata Katsumi , Ohyanagi Hajime , Sato Shinji , Nobori Hiroya , Hayashi Akiko , Ishii Hideshi , Daub Carsten O. , Kawai Jun , Suzuki Harukazu , Saito Toshiyuki 
Published: February 2015

AbstractWe present a system-wide transcriptional network structure that controls cell types in the context of expression pattern transitions that correspond to cell type transitions. Co-expression based analyses uncovered a system-wide, ladder-like transcription factor cluster structure composed of nearly 1,600 transcription factors in a human transcriptional network. Computer simulations based on a transcriptional regulatory model deduced from the system-wide, ladder-like transcription factor cluster structure reproduced expression pattern transitions when human THP-1 myelomonocytic leukaemia cells cease proliferation and differentiate under phorbol myristate acetate stimulation. The behaviour of MYC, a reprogramming Yamanaka factor that was suggested to be essential for induced pluripotent stem cells during dedifferentiation, could be interpreted based on the transcriptional regulation predicted by the system-wide, ladder-like transcription factor cluster structure. This study introduces a novel system-wide structure to transcriptional networks that provides new insights into network topology.