bluuebunny/crossref_metadata_2025_split huggingface dataset:
A series of quinazolin‐4(3H)‐one‐morpholine hybrids as anti‐lung‐cancer agents: Synthesis, molecular docking, molecular dynamics, ADME prediction and biological activity studies
Authors: Tokalı Feyzi Sinan , Şenol Halil , Ateşoğlu Şeyma , Akbaş Fahri 
Published: July 2024

AbstractIn this study, we synthesized 15 novel quinazoline‐morpholinobenzylideneamino hybrid compounds from methyl anthranilate and we assessed their cytotoxicity via in vitro assays against A549 and BEAS‐2B cell lines. Molecular docking studies were conducted to evaluate the protein‐ligand interactions and inhibition mechanisms on nine different molecular targets, while molecular dynamics (MD) simulations were carried out to assess the stability of the best docked ligand–protein complexes. Additionally, ADME prediction was carried out to determine physicochemical parameters and drug likeness. According to the cytotoxicity assays, compound 1 (IC50 = 2.83 μM) was found to be the most active inhibitor against A549 cells. While the selectivity index (SI) of compound 1 is 29, the SI of the reference drugs paclitaxel and sorafenib, used in this study, are 2.40 and 4.92, respectively. Among the hybrid compounds, 1 has the best docking scores against VEGFR1 (−11.744 kcal/mol), VEGFR2 (−12.407 kcal/mol) and EGFR (−10.359 kcal/mol). During MD simulations, compound 1 consistently exhibited strong hydrogen bond interactions with the active sites of VEGFR1 and 2, and these interactions were maintained for more than 90% of the simulation time. Additionally, the RMSD and RMSF values of the ligand–protein complexes exhibited high stability at their minimum levels around 1–2 Å. In conclusion, these findings suggest that compound 1 may be a potent and selective inhibitor candidate for lung cancer treatment and inhibition of VEGFR2, especially.
Mechanism of ferroptosis regulating ischemic stroke and pharmacologically inhibiting ferroptosis in treatment of ischemic stroke
Authors: Chai Zhaohui , Zheng Jiesheng , Shen Jian 
Published: July 2024

AbstractFerroptosis is a newly discovered form of programmed cell death that is non‐caspase‐dependent and is characterized by the production of lethal levels of iron‐dependent lipid reactive oxygen species (ROS). In recent years, ferroptosis has attracted great interest in the field of cerebral infarction because it differs morphologically, physiologically, and genetically from other forms of cell death such as necrosis, apoptosis, autophagy, and pyroptosis. In addition, ROS is considered to be an important prognostic factor for ischemic stroke, making it a promising target for stroke treatment. This paper summarizes the induction and defense mechanisms associated with ferroptosis, and explores potential treatment strategies for ischemic stroke in order to lay the groundwork for the development of new neuroprotective drugs.
Tracking species recovery status to improve U.S. endangered species act decisions
Authors: Davis Olivia N. , Molano‐Flores Brenda , Li Ya‐Wei , Allen Maximilian L. , Davis Mark A. , Mengelkoch Jean M. , Parkos Joseph J. , Porreca Anthony Paul , Fournier Auriel M. V. , Tiemann Jeremy , Bried Jason , Marcum Paul B. , Carroll‐Cunningham Connie J. , Janssen Eric D. , Ulaszek Eric F. , McIntyre Susan , Price Edward P. F. , Nieset Julie , Beveroth Tara , Di Giovanni Alexander , Askren Ryan J. , Malanchuk Luke J. , Duquette Jared F. , Dreslik Michael J. , McElrath Thomas C. , Stodola Kirk , Malcom Jacob , Carter Andrew , Evansen Megan , Gerber Leah R. 
Published: July 2024

AbstractCurrently 1677 species are listed under the U.S. Endangered Species Act (ESA), yet only a small percentage have been delisted due to recovery. In the fall of 2021, the U.S. Fish and Wildlife Service proposed delisting 23 species due to extinction. Tracking changes in species ‘recovery status over time is critical to understanding species’ statuses, informing adaptive management strategies, and assessing the performance of the ESA to prevent further species loss. In this paper, we describe four key obstacles in tracking species recovery status under the ESA. First, ESA 5‐year reviews lack a standardized format and clear documentation. Second, despite having been listed for decades, many species still suffer major data gaps in their biology and threats, rendering it difficult if not impossible to track progress towards recovery. Third, many species have continued declining after listing, yet given the above (1 & 2), understanding potential causes (proximate and/or ultimate) can be difficult. Fourth, many species currently have no path to clear recovery, which represents a potential failing of the process. We conclude with a discussion of potential policy responses that could be addressed to enhance the efficacy of the ESA.
Individual treatment effects of sodium‐glucose co‐transporter‐2 inhibitors on the risk of chronic kidney disease in patients with type 2 diabetes: A counterfactual prediction model based on real‐world data
Authors: Siriyotha Sukanya , Lukkunaprasit Thitiya , Looareesuwan Panu , Kunakorntham Patratorn , Anothaisintawee Thunyarat , Nimitphong Hataikarn , McKay Gareth J. , Attia John , Thakkinstian Ammarin 
Published: July 2024

AbstractAimTo estimate individual treatment effects (ITEs) of sodium‐glucose co‐transporter‐2 inhibitors (SGLT2is) on lowering the risk of developing chronic kidney disease (CKD) in patients with type 2 diabetes (T2D) and to identify those most probable to benefit from treatment.MethodsThis study followed a T2D cohort from Ramathibodi Hospital, Thailand, from 2015 to 2022. A counterfactual model was constructed to predict factual and counterfactual risks of CKD if patients did/did not receive SGLT2is. ITEs were estimated by subtracting the factual risk from the counterfactual risk of CKD.ResultsThere were 1619 and 15 879 patients included in the SGLT2i and non‐SGLT2i groups, respectively. The estimated ITEs varied from −1.19% to −17.51% with a median of −4.49%, that is, 50% of patients had a 4.49% or greater lower CKD risk if they received an SGLT2i. Patients who gained the greatest benefit from SGLT2is were more probable to be male, aged at least 60 years, with a history of diabetes duration of at least 3 months, hypertension, peripheral arterial disease, diabetic retinopathy and low high‐density lipoprotein cholesterol.ConclusionsOur prediction model provides individualized information that helps target T2D patients who may benefit more from SGLT2is. This could help clinical decision making and implementation of personalized medicine in clinical practice, especially in resource‐limited settings.
Melanoma and cannabinoids: A possible chance for cancer treatment
Authors: Galeano Mariarosaria , Vaccaro Federico , Irrera Natasha , Caradonna Emanuela , Borgia Francesco , Li Pomi Federica , Squadrito Francesco , Vaccaro Mario 
Published: July 2024

AbstractThe endocannabinoid system is composed by a complex and ubiquitous network of endogenous lipid ligands, enzymes for their synthesis and degradation, and receptors, which can also be stimulated by exogenous compounds, such as those derived from the Cannabis sativa. Cannabis and its bioactive compounds, including cannabinoids and non‐cannabinoids, have been extensively studied in different conditions. Recent data have shown that the endocannabinoid system is responsible for maintaining the homeostasis of various skin functions such as proliferation, differentiation and release of inflammatory mediators. Because of their role in regulating these key processes, cannabinoids have been studied for the treatment of skin cancers and melanoma; their anti‐tumour effects regulate skin cancer progression and are mainly related to the inhibition of tumour growth, proliferation, invasion and angiogenesis, through apoptosis and autophagy induction. This review aims at summarising the current field of research on the potential uses of cannabinoids in the melanoma field.
Comparative study of optimal compression pressure between conventional Barton's dressing and prefabricate garment in parotidectomy patients
Authors: Kertpholwattana Supanut , Pongsapich Warut , Ratanaprasert Narin , Ngamying Nuttapol , Thamaphat Kheamrutai 
Published: July 2024

AbstractExcessive compression after parotidectomy can lead to flap necrosis, while inadequate pressure can cause fluid accumulation. This study aimed to determine the optimal pressure and compression properties of different types of dressings. Initially, pressure measurements were taken for conventional Barton's dressing and a pre‐fabricated facial garment. In the subsequent phase, patients were randomly assigned to receive one of three types of pressure dressings: conforming bandage Barton's dressing, elastic bandage Barton's dressing or pre‐fabricated facial garment. The dressing types were randomly crossed over the following day. The mean pressure exerted by conventional Barton's dressing and the pre‐fabricated facial garment was 15.86 and 14.81 mmHg, respectively. There was no significant difference in the proportion of optimal pressure among the three types of pressure dressing (p‐values of 0.195, 0.555 and 0.089 at pre‐auricular, angle of mandible and post‐auricular sites, respectively). The pre‐auricular area demonstrated the highest proportion of optimal pressure, while suboptimal pressure was noted at the angle of the mandible and post‐auricular area. Dressing types had no effect on pressure stability (p = 0.37), and there was no significant difference in patient preference (p = 0.91). Conforming bandage Barton's dressing, elastic bandage Barton's dressing and pre‐fabricated facial garment exhibit comparable compressive properties, with no significant difference in patient preference and pressure stability.
Phase transition in Eu2Zr2–xO7–2x (x = 0–1) solid solutions: A combined structural and spectroscopic study
Authors: Kong Linggen , Wang Zhiyang , Karatchevtseva Inna , Zhang Yingjie 
Published: July 2024

AbstractZirconate pyrochlores have been extensively studied as candidate waste forms for the immobilization of actinide wastes due to their flexible crystal chemistry, excellent structural stability, and exceptional radiation tolerance. Herein, we report the synthesis of a series of compounds Eu2Zr2–xO7–2x (x = 0–1, step = 0.125) using a co‐precipitation route to form solid solutions between the two end members, Eu2Zr2O7 pyrochlore and Eu2ZrO5 defect fluorite. It is important to understand the pyrochlore to defect fluorite transformation as a function of Zr/Ln molar ratio. The structural investigation by x‐ray diffraction has shown the increasing cation disorder and corresponding structural transitions from the ordered pyrochlore to disordered pyrochlore, then to defect fluorite. While diffuse reflectance spectroscopy provides similar optical absorption features reflecting the presence of Eu(III) ion, Raman spectroscopy has revealed more details on the phase transition and short‐range structures. This work highlights the structural flexibility in zirconate materials as potential nuclear waste forms for the immobilization of actinide‐rich waste streams, especially suitable for the separated minor actinides.
Association between COVID‐19 vaccination and atrial arrhythmias in individuals with cardiac implantable electronic devices
Authors: Deshmukh Abhishek J. , Ahmad Rimsha , Cha Yong‐Mei , Mulpuru Siva K. , DeSimone Christopher V. , Killu Ammar M. , Mullane Steven , Harrell Camden , Kutyifa Valentina , Cheung Jim W. , Upadhyay Gaurav A. , Piccini Jonathan P. , Hayes David L. , Madhavan Malini 
Published: July 2024

AbstractIntroductionThe impact of mRNA‐based coronavirus disease‐2019 (COVID‐19) vaccines on atrial arrhythmias (AA) and ventricular arrhythmias incidence is unknown.MethodsBIOTRONIK Home Monitoring data and Medicare Claims data were utilized to identify individuals implanted with a cardiac implantable electronic device (CIED) between 2010 and 2020 who received one or more doses of COVID‐19 vaccine in 2021. The burden of AA (%) in the 3 months postvaccination was compared to those noted in the preceding 3 months using the Wilcoxon signed rank test. Sub‐analyses comparing the effects of the influenza vaccine against the COVID‐19 vaccine were also evaluated for individuals who received the influenza vaccine in 2020. A 1:1 propensity score match comparison between COVID‐19 vaccine and non‐vaccinated patients was also performed.ResultsFirst and second doses of the COVID‐19 vaccine were administered to 7757 and 6579 individuals with a CIED (age 76.2 ± 9.0 years, 49% males), respectively. While a small but statistically significant increase in the burden of AA was noted in the 3 months postvaccination compared to the preceding 3 months after the first dose of the COVID‐19 vaccine (0.43 ± 9.04%, p = .028) a similar rise in AA was found following the influenza vaccine and for matched patients who did not receive the COVID‐19 vaccine. No significant difference in device therapies was seen pre‐ and postvaccination.ConclusionsThough we report a small but significant increase in the number of CIED‐detected AAs following vaccination for COVID‐19 over a 3‐month window, we believe these results correlate more with time and the progressive nature of AF rather than the vaccine itself. While these data should not dissuade from the use of these vaccines, increased vigilance and prompt treatment of AF is required for high‐risk groups, specifically males over 70 years of age, following vaccination.
Puerarin inhibits Staphylococcus aureus‐induced endometritis through attenuating inflammation and ferroptosis via regulating the P2X7/NLRP3 signalling pathway
Authors: Yuan Chongshan , Liu Li , Zhao Ye , Wang Ke 
Published: July 2024

AbstractEndometritis is one of the important causes of infertility. Puerarin (PU) can inhibit oxidative stress and reduce inflammation; however, it is unclear whether PU has a protective effect on the endometritis. In our study, we used Staphylococcus aureus to induce mouse endometritis. The PU group (100 mg/kg PU) and the S. aureus + PU group received daily intraperitoneal injection of PU (25, 50 or 100 mg/kg PU). The results showed that S. aureus significantly increased the levels of MPO, TNF‐α, IL‐1β and IL‐6 in uterine tissue, and increased the expression of p‐p65 and p‐IκBα proteins in uterine tissue to induce endometritis in mice (p < 0.05). Furthermore, it has been found that S. aureus promotes the occurrence of ferroptosis by reducing GSH and ATP content, increasing MDA and iron content and reducing GPX4 and SLC7A11 protein expression levels (p < 0.05). S. aureus significantly increase the expression of NLRP3, ASC, caspase‐1 and P2X7 proteins in uterine tissue (p < 0.05). However, PU obviously reduced the inflammatory response and reversed the changes of ferroptosis and the expression of P2X7 receptor/NLRP3 pathway associated proteins of the uterus induced by S. aureus (p < 0.05). Taken together, these findings emphasize the protective effect of PU on endometritis by regulating the P2X7 receptor/NLRP3 signalling pathway and inhibiting ferroptosis.
Hypomethylation‐associated Sox11 upregulation promotes oncogenesis via the PI3K/AKT pathway in OLP‐associated OSCC
Authors: Liu Yi , Cao Peilin , Xiao Li , Tang Na , Fei Wei , Li Xue 
Published: July 2024

AbstractOral lichen planus (OLP) is a particularly prevalent oral disorder with the potential to progress to oral squamous cell carcinoma (OSCC). SRY‐box transcription factor 11 (Sox11) has been reported to serve as a prognostic marker for various cancers. However, the role and mechanism of Sox11 in OLP‐related OSCC are unknown. Our results indicated that Sox11 was highly expressed, and that Sox11 promoter methylation was significantly reduced in OLP‐associated OSCC tissues. High Sox11 expression and Sox11 promoter hypomethylation indicate a poor patient prognosis. According to in vivo and in vitro experiments, the knockdown of Sox11 inhibited proliferation, invasion, and migration while driving its apoptotic death in OSSC cells; Sox11 overexpression exerted the opposite effect as Sox11 knockdown. Mechanistically, knockdown of Sox11 inhibited PI3K/AKT and glycolysis pathway, and overexpression of Sox11 enhanced the PI3K/AKT and glycolysis pathways in OSCC cells. In addition, we demonstrated that Sox11 overexpression accelerated the progression of OSCC, at least in part by promoting PI3K/AKT pathway activation. In conclusion, our data indicated that the DNA hypomethylation‐associated upregulation of Sox11 could promote oncogenic transformation via the PI3K/AKT pathway in OLP‐associated OSCC. Therefore, Sox11 might be a reliable biomarker for predicting the progression of precancerous oral tissues.
Cover Image
Authors: Marruganti Crystal , Gaeta Carlo , Falciani Chiara , Cinotti Elisa , Rubegni Pietro , Alovisi Mario , Scotti Nicola , Baldi Andrea , Bellan Cristiana , Defraia Chiara , Fiorino Fabio , Valensin Silvia , Bellini Erika , De Rosa Antonella , D'Aiuto Francesco , Grandini Simone 
Published: July 2024

The cover image is based on the Original Article Are periodontitis and psoriasis associated? A pre‐clinical murine model by Crystal Marrugant et al., https://doi.org/10.1111/jcpe.13996.image
Impact of molecular and histopathological findings on FIGO 2009 stage I endometrial cancer: Transition to FIGO 2023 staging system
Authors: Öncü Hande Nur , Ersak Burak , Ege Gökçen , Öztürk Neslihan , Yeşil Baran , Köksal Oğuz Kaan , Duran Firdevs Şahin , Hanedan Candost , Soysal Çağanay , Korkmaz Vakkas 
Published: July 2024

AbstractAimThis study aims to investigate the impact of integrating molecular and histopathological findings into the revised International Federation of Gynecology and Obstetrics (FIGO) 2023 staging system on patients initially diagnosed with stage I endometrial cancer (EC) according to the FIGO 2009 criteria.MethodsA cohort of 197 EC patients, initially classified as stage I under FIGO 2009, underwent restaging based on the updated FIGO 2023 criteria. The patients' molecular and histopathological characteristics were documented, and their impact on upstaging was analyzed.ResultsMolecular profiling was conducted for 81.2% (160/197) of the patients, revealing that 55.3% (109/197) were classified as non‐specific molecular profile, 14.7% (29/197) as mismatch repair deficiency, 11.2% (22/197) as p53 abnormality (p53abn), and 18.8% (37/197) as unknown. Upstaging was identified in 26.9% (43/160) of the 160 patients with known molecular profiles. Among the upstaged patients, 51.2% experienced upstaging due to p53 abnormality, 20.9% due to substantial lymphovascular space invasion (LVSI), 20.9% due to aggressive histological types, and 6.9% due to high grade.ConclusionsThe introduction of the molecular profile into the revised FIGO 2023 staging system for stage I EC has led to notable changes in the staging of approximately one‐fifth of patients. While p53 abnormalities have emerged as the most influential factor contributing to the upstaging, LVSI and aggressive histological types also represent significant contributing factors.
Identification, characterization and classification of prokaryotic nucleoid‐associated proteins
Authors: Schwab Samuel , Dame Remus T. 
Published: July 2024

AbstractCommon throughout life is the need to compact and organize the genome. Possible mechanisms involved in this process include supercoiling, phase separation, charge neutralization, macromolecular crowding, and nucleoid‐associated proteins (NAPs). NAPs are special in that they can organize the genome at multiple length scales, and thus are often considered as the architects of the genome. NAPs shape the genome by either bending DNA, wrapping DNA, bridging DNA, or forming nucleoprotein filaments on the DNA. In this mini‐review, we discuss recent advancements of unique NAPs with differing architectural properties across the tree of life, including NAPs from bacteria, archaea, and viruses. To help the characterization of NAPs from the ever‐increasing number of metagenomes, we recommend a set of cheap and simple in vitro biochemical assays that give unambiguous insights into the architectural properties of NAPs. Finally, we highlight and showcase the usefulness of AlphaFold in the characterization of novel NAPs.
Testosterone in prevention and treatment of type 2 diabetes in men: Focus on recent randomized controlled trials
Authors: Grossmann Mathis , Wittert Gary A. 
Published: July 2024

AbstractIn epidemiological studies, lowered serum testosterone concentrations are common in men with obesity, prediabetes, and established type 2 diabetes (T2D). In men with prediabetes, lowered serum testosterone also predicts a future risk of T2D in men. Administration of testosterone consistently reduces fat mass and increases skeletal muscle mass—body compositional changes expected to be metabolically favorable. In men with established T2D, the effects of testosterone treatment on glycemic measures are inconsistent. Irrespective of baseline serum testosterone concentration in men with prediabetes or newly diagnosed early‐onset T2D, testosterone treatment prescribed in conjunction with a lifestyle program has been reported to reduce the risk of T2D by 40% after 2 years, suggesting that either a lifestyle program is required to facilitate the glycemic benefit of testosterone treatment and/or that testosterone treatment has more favorable effects on glycemia in men early in the evolution or onset of the disease. The durability of the benefit and longer‐term safety of testosterone treatment have not been established. Therefore, more studies are required before testosterone treatment can be recommended for the prevention and/or treatment of men with or at elevated risk of T2D who do not have hypogonadism due to an established disease of the hypothalamic‐pituitary‐testicular axis.
Attention‐deficit/hyperactivity disorder in adolescent and adult candidates for metabolic and bariatric surgery: A systematic review and meta‐analysis
Authors: Caci Hervé , Chierici Andrea , Iannelli Antonio 
Published: July 2024

SummaryAimsAttention‐deficit with hyperactivity disorder (ADHD) is associated with obesity and impacts the outcome of metabolic and bariatric surgery (MBS). This study aimed at calculating the prevalence in candidates for MBS, which is yet unclear.MethodsWe conducted a systematic review and meta‐analysis, searching three databases from their respective inception to December 2022 for studies reporting the prevalence of ADHD in adolescents and adults assessed before undergoing MBS. The protocol was registered in PROSPERO (CRD42022384914). We adhered to Meta‐analysis of Observational Studies in Epidemiology (MOOSE) and Preferred Reporting Items for Systematic Reviews and Meta‐Analyses (PRISMA) 2020 guidelines; the quality of studies was assessed with the JBI Critical Appraisal checklist. Random‐effect meta‐analyses were performed; confidence intervals were computed with a binomial exact method, and the pooled estimate was calculated after double arcsine transformation.FindingsFourteen studies (24,455 adults) and three studies (299 adolescents) were selected. The quality of studies was moderate to poor; meta‐analyses were performed on subgroups according to the case definition used. The prevalence is 8.94% and 9.90% in adults, and 28.73% in adolescents.ConclusionsADHD is three times more frequent in adults and six times more frequent in adolescents than in the general population. Recommendations are provided to improve the quality of future studies and obtain more reliable estimates of prevalence.