bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering the tumour microenvironment of clear cell renal cell carcinoma: Prognostic insights from programmed death genes using machine learning
Authors: Tu Hongtao , Hu Qingwen , Ma Yuying , Huang Jinbang , Luo Honghao , Jiang Lai , Zhang Shengke , Jiang Chenglu , Lai Haotian , Liu Jie , Chen Jianyou , Guo Liwei , Yang Guanhu , Xu Ke , Chi Hao , Chen Haiqing 
Published: July 2024

AbstractClear cell renal cell carcinoma (ccRCC), a prevalent kidney cancer form characterised by its invasiveness and heterogeneity, presents challenges in late‐stage prognosis and treatment outcomes. Programmed cell death mechanisms, crucial in eliminating cancer cells, offer substantial insights into malignant tumour diagnosis, treatment and prognosis. This study aims to provide a model based on 15 types of Programmed Cell Death‐Related Genes (PCDRGs) for evaluating immune microenvironment and prognosis in ccRCC patients. ccRCC patients from the TCGA and arrayexpress cohorts were grouped based on PCDRGs. A combination model using Lasso and SuperPC was constructed to identify prognostic gene features. The arrayexpress cohort validated the model, confirming its robustness. Immune microenvironment analysis, facilitated by PCDRGs, employed various methods, including CIBERSORT. Drug sensitivity analysis guided clinical treatment decisions. Single‐cell data enabled Programmed Cell Death‐Related scoring, subsequent pseudo‐temporal and cell–cell communication analyses. A PCDRGs signature was established using TCGA‐KIRC data. External validation in the arrayexpress cohort underscored the model's superiority over traditional clinical features. Furthermore, our single‐cell analysis unveiled the roles of PCDRG‐based single‐cell subgroups in ccRCC, both in pseudo‐temporal progression and intercellular communication. Finally, we performed CCK‐8 assay and other experiments to investigate csf2. In conclusion, these findings reveal that csf2 inhibit the growth, infiltration and movement of cells associated with renal clear cell carcinoma. This study introduces a PCDRGs prognostic model benefiting ccRCC patients while shedding light on the pivotal role of programmed cell death genes in shaping the immune microenvironment of ccRCC patients.
Erythropoietic protoporphyrias: Pathogenesis, diagnosis and management
Authors: Minder Anna‐Elisabeth , Kluijver Louisa G. , Barman‐Aksözen Jasmin , Minder Elisabeth I. , Langendonk Janneke G. 
Published: July 2024

AbstractThe erythropoietic protoporphyrias consist of three ultra‐rare genetic disorders of the erythroid heme biosynthesis, including erythropoietic protoporphyria (EPP1), X‐linked protoporphyria (XLEPP) and CLPX‐protoporphyria (EPP2), which all lead to the accumulation of protoporphyrin IX (PPIX) in erythrocytes. Affected patients usually present from early childhood with episodes of severe phototoxic pain in the skin exposed to visible light. The quantification of PPIX in erythrocytes with a metal‐free PPIX ≥3 times the upper limit of normal confirms the diagnosis. Protoporphyria‐related complications include liver failure, gallstones, mild anaemia and vitamin D deficiency with reduced bone mineral density. The management is focused on preventing phototoxic reactions and treating the complications. Vitamin D should be supplemented, and DEXA scans in adults should be considered. In EPP1, even in cases of biochemically determined iron deficiency, supplementation of iron may stimulate PPIX production, resulting in an increase in photosensitivity and the risk of cholestatic liver disease. However, for patients with XLEPP, iron supplementation can reduce PPIX levels, phototoxicity and liver damage. Because of its rarity, there is little data on the management of EPP‐related liver disease. As a first measure, any hepatotoxins should be eliminated. Depending on the severity of the liver disease, phlebotomies, exchange transfusions and ultimately liver transplantation with subsequent haematopoietic stem cell transplantation (HSCT) are therapeutic options, whereby multidisciplinary management including porphyria experts is mandatory. Afamelanotide, an alpha‐melanocyte‐stimulating hormone analogue, is currently the only approved specific treatment that increases pain‐free sunlight exposure and quality of life.
Holmium laser enucleation of the prostate (HoLEP) in short‐circuit outpatient care: Is prostatic volume a limiting factor?
Authors: Maria Sánchez Rodríguez , Franco Marco , Freixa Sala Roger , Gasanz Serrano Carlos , Bernardello Ureta Mauro , Bultó Gonzalvo Ramón , Cervera Alcaide Jordi , Casanova García Carla , García Puche Mireia , Segura Alabart Maria , Areal Calama Juan José , Ágreda Castañeda Fernando 
Published: July 2024

AbstractIntroduction and ObjectivesOur goals were to study prostatic volume as a limiting factor after HoLEP surgery with short‐circuit outpatient care (4 h) and to define other factors that affect the success of the proposed circuit.Materials and MethodsAn observational analysis and review was performed using a prospective database. Preoperative, intraoperative, and postoperative variables were included for patients who were scheduled for short‐circuit outpatient care (SCOC) and who underwent HoLEP between 2020 and 2023. We defined SCOC as a postoperative hospital stay of 4 h. Subjects who required more than 4 h in hospital were categorized as conventional hospital admission (CHA). A descriptive populational study was conducted, expressing the mean using a 95% confidence interval and percentages for the continuous variables. In order to analyze them, we used the Student's t‐test for the continuous variables and the chi‐squared test for the categorical variables.ResultsSixty‐eight patients were included, 54 of which completed SCOC, which represented a success ratio of 79.5%. The mean age and prostatic volume of the whole cohort were 68.9 (±6.8) years and 79.5 (±29.1) mL, respectively. We found no significant differences in age, prostatic volume, antiplatelet drug use, indwelling bladder catheter, or applied energy among the subjects who completed SCOC and those who required CHA. No patient was presented with a complication of Grade 3 (or higher) in the modified Clavien–Dindo classification. At the six‐month follow‐up, no differences were observed in the uroflowmetry or International Prostate Symptoms Score variables.ConclusionsProstatic volume does not seem to be a limiting factor after undergoing HoLEP with short‐circuit outpatient care.
Cutaneous fusarium disease and leukaemias: A systematic review
Authors: Cortés‐López Paulina Nundehui , Guzmán‐Montijo Estefanía , Fuentes‐Venado Claudia Erika , Arenas Roberto , Bonifaz Alexandro , Pinto‐Almazán Rodolfo , Martínez‐Herrera Erick 
Published: July 2024

AbstractThe present study analyses the clinical characteristics of patients diagnosed with cutaneous fusarium through a systematic review of cases reported in literature. A total of 39 cases were included, of which 53% were men, 30% were women, and in 17% the sex was not specified. The age ranged from 5 to 85 years. Most cases were reported in Brazil, followed by Japan and United States of America. The most common agent was Fusarium solani, in 37.5% of the patients. Most of the affected individuals had acute myeloid leukaemia and some of the predisposing factors, which included induction chemotherapy, febrile neutropenia, and bone marrow transplantation. The clinical topography of the lesions was located in 27.5% and disseminated in 72.5%, with the most observed clinical feature outstanding the presence of papules and nodules with central necrosis in 47% of the cases. Longer survival was demonstrated in those treated with more than three antifungals. It is concluded that cutaneous fusarium is a complex and challenging clinical entity, infection in patients with leukaemias underscores the need for thorough care to decrease morbidity and mortality.
Evaluating the efficacy of few‐shot learning for GPT‐4Vision in neurodegenerative disease histopathology: A comparative analysis with convolutional neural network model
Authors: Ono Daisuke , Dickson Dennis W. , Koga Shunsuke 
Published: July 2024

AbstractAimsRecent advances in artificial intelligence, particularly with large language models like GPT‐4Vision (GPT‐4V)—a derivative feature of ChatGPT—have expanded the potential for medical image interpretation. This study evaluates the accuracy of GPT‐4V in image classification tasks of histopathological images and compares its performance with a traditional convolutional neural network (CNN).MethodsWe utilised 1520 images, including haematoxylin and eosin staining and tau immunohistochemistry, from patients with various neurodegenerative diseases, such as Alzheimer's disease (AD), progressive supranuclear palsy (PSP) and corticobasal degeneration (CBD). We assessed GPT‐4V's performance using multi‐step prompts to determine how textual context influences image interpretation. We also employed few‐shot learning to enhance improvements in GPT‐4V's diagnostic performance in classifying three specific tau lesions—astrocytic plaques, neuritic plaques and tufted astrocytes—and compared the outcomes with the CNN model YOLOv8.ResultsGPT‐4V accurately recognised staining techniques and tissue origin but struggled with specific lesion identification. The interpretation of images was notably influenced by the provided textual context, which sometimes led to diagnostic inaccuracies. For instance, when presented with images of the motor cortex, the diagnosis shifted inappropriately from AD to CBD or PSP. However, few‐shot learning markedly improved GPT‐4V's diagnostic capabilities, enhancing accuracy from 40% in zero‐shot learning to 90% with 20‐shot learning, matching the performance of YOLOv8, which required 100‐shot learning to achieve the same accuracy.ConclusionsAlthough GPT‐4V faces challenges in independently interpreting histopathological images, few‐shot learning significantly improves its performance. This approach is especially promising for neuropathology, where acquiring extensive labelled datasets is often challenging.
BRIC in flux: Understanding the influence of energy policy uncertainty on foreign direct investment flows
Authors: Tabash Mosab I. , Farooq Umar , Issa Suzan Sameer 
Published: July 2024

AbstractThis research investigates the intricate relationship between energy policy uncertainty (EGU) and foreign direct investment (FDI) within the BRIC economies over a comprehensive 27‐year period, spanning from 1996 to 2022. Employing advanced econometric techniques such as FMOLS and DOLS for regression analysis, the study unravels the nuanced impacts of EGU on both FDI inflows (IFD) and outflows (OFD). Drawing upon a comprehensive data set, the analysis reveals a significant negative correlation between EGU and IFD, indicating that heightened energy policy uncertainties deter foreign capital from entering BRICS nations. Contrarily, the study unveils a paradoxical positive relationship between EGU and OFD, suggesting that energy policy uncertainties stimulate the outflow of FDI, reflecting the adaptive strategies of multinational corporations navigating uncertainties. The study further explores the role of government effectiveness, labour force and financial sector development, shedding light on their positive influences on IFD and negative impacts on OFD. These findings underscore the importance of effective governance, a skilled labour force, and financial sector development in attracting and retaining foreign investments. This research contributes to the literature on energy policy, governance and FDI, offering policymakers and businesses nuanced insights into crafting strategies that enhance the attractiveness of BRIC nations for foreign investments. The study's findings have implications for shaping stable energy policies, improving governance effectiveness and fostering conditions conducive to sustained economic growth and development.
Heat waves induce milkweed resistance to a specialist herbivore via increased toxicity and reduced nutrient content
Authors: López‐Goldar Xosé , Mollema Alyssa , Sivak‐Schwennesen Caz , Havko Nathan , Howe Gregg , Agrawal Anurag A. , Wetzel William C. 
Published: July 2024

AbstractOver the last decade, a large effort has been made to understand how extreme climate events disrupt species interactions. Yet, it is unclear how these events affect plants and herbivores directly, via metabolic changes, and indirectly, via their subsequent altered interaction. We exposed common milkweed (Asclepias syriaca) and monarch caterpillars (Danaus plexippus) to control (26:14°C, day:night) or heat wave (HW) conditions (36:24°C, day:night) for 4 days and then moved each organism to a new control or HW partner to disentangle the direct and indirect effects of heat exposure on each organism. We found that the HW directly benefited plants in terms of growth and defence expression (increased latex exudation and total cardenolides) and insect her'bivores through faster larval development. Conversely, indirect HW effects caused both plant latex and total cardenolides to decrease after subsequent herbivory. Nonetheless, increasing trends of more toxic cardenolides and lower leaf nutritional quality after herbivory by HW caterpillars likely led to reduced plant damage compared to controls. Our findings reveal that indirect impacts of HWs may play a greater role in shaping plant‐herbivore interactions via changes in key physiological traits, providing valuable understanding of how ecological interactions may proceed in a changing world.
Rice CRYPTOCHROME‐INTERACTING BASIC HELIX‐LOOP‐HELIX 1‐LIKE interacts with OsCRY2 and promotes flowering by upregulating Early heading date 1
Authors: Lee Sang‐Ji , Kim Yunjeong , Kang Kiyoon , Yoon Hyeryung , Kang Jinku , Cho Sung‐Hwan , Paek Nam‐Chon 
Published: July 2024

AbstractFlowering time is a crucial adaptive response to seasonal variation in plants and is regulated by environmental cues such as photoperiod and temperature. In this study, we demonstrated the regulatory function of rice CRYPTOCHROME‐INTERACTING BASIC HELIX‐LOOP‐HELIX 1‐LIKE (OsCIBL1) in flowering time. Overexpression of OsCIB1L promoted flowering, whereas the oscib1l knockout mutation did not alter flowering time independent of photoperiodic conditions. Cryptochromes (CRYs) are blue light photoreceptors that enable plants to sense photoperiodic changes. OsCIBL1 interacted with OsCRY2, a member of the rice CRY family (OsCRY1a, OsCRY1b, and OsCRY2), and bound to the Early heading date 1 (Ehd1) promoter, activating the rice‐specific Ehd1‐Heading date 3a/RICE FLOWERING LOCUS T 1 pathway for flowering induction. Dual‐luciferase reporter assays showed that the OsCIBL1‐OsCRY2 complex required blue light to induce Ehd1 transcription. Natural alleles resulting from nonsynonymous single nucleotide polymorphisms in OsCIB1L and OsCRY2 may contribute to the adaptive expansion of rice cultivation areas. These results expand our understanding of the molecular mechanisms controlling rice flowering and highlight the importance of blue light‐responsive genes in the geographic distribution of rice.
Technology‐based parent school: A randomized controlled trial
Authors: Cirlak Ahu , Kilicarslan Ebru 
Published: July 2024

AbstractObjectiveThis study was conducted as a randomized controlled trial to evaluate the technology‐based parent school program.DesignThe study was conducted in the well‐child care outpatient clinic of a hospital in Turkey. Data were collected between October 31, 2022 and February 17, 2023. The parent school was prepared based on Meleis' transition theory.SampleForty‐eight parents participated in the study. Parents in the intervention group participated in a 10‐week parent school program (five website modules, five online interactive group trainings, and counseling).MeasuresData were collected before the program, after the program, and 1 month after the program.ResultsThere were increases in the mean scores of the “Parent Skill Assessment Form” and “Skills Assessment Form for the Toddler Development” items in the intervention group compared to the control group. The difference between the groups in the mean scores of the Self‐Efficacy for Parenting Task Index Toddler Scale (1–3 years) was not statistically significant. However, the difference between the times was statistically significant.ConclusionIt was concluded that the technology‐based parent school training program could guide parents with children aged 1–3 years on child‐rearing.Trial registrationIt was registered at ClinicalTrials.gov in May 2022 (NCT05370989).
Christianity and modern Japanese society: An historical overview
Authors: Tao Bo 
Published: July 2024

AbstractThis review article provides an overview of studies on Christianity in modern Japan, with a specific focus on works that address its social and political impact from a historical perspective. It first seeks to orient readers in the historical context within which the religion was transmitted to Japan, before delving into the notable works in the field. The scholarship indicates an evolution from an initial focus on missionary evangelism and narrowly defined church history to a more expansive outlook that encompasses the rich and complex lives of Japanese Christian leaders and their broader societal impact beyond church walls. Topics covered include Protestant missions, indigenous movements, women's education, social reform, and biographical studies. As a reflection of the author's area of interest, special attention will be paid to the case of the Christian social reformer Kagawa Toyohiko. The review will be limited to English‐language scholarship, although brief references will be made to Japanese‐language scholarship where appropriate.
Analyzing the effects of climate risk on discouraged borrowers: Deciphering the contradictory forces
Authors: Anastasiou Dimitris , Ballis Antonis , Kallandranis Christos , Lakhal Faten 
Published: July 2024

AbstractWe examine the impact of climate risk on discouraged borrowers among small and medium‐sized enterprises (SMEs) in the eurozone, using a unique European Central Bank dataset focusing on the demand side of credit markets. We argue that two opposing channels may exist in this relationship: Either climate risk has a negative effect stemming from increased demand for sustainable or climate‐resilient projects that enhance creditworthiness, or climate risk has a positive effect arising from heightened climate uncertainty and risk aversion, leading to credit self‐rationing among SMEs. Our findings reveal that heightened climate risk prompts SMEs to self‐ration credit, leading to higher probabilities of discouraged borrowers. Our research deepens the understanding of the impact of climate risk on credit‐related decisions, stressing the need for proactive measures to integrate climate risk assessments into regulatory frameworks and lending practices. The findings underscore the vulnerability of SMEs to climate risk, emphasizing emphasizing the importance of tailored support mechanisms for economic resilience.
Exploring electron backscatter diffraction analysis as a tool for understanding stromatolite: Quantitative description of Cretaceous lacustrine stromatolite reveals formative processes and high‐resolution climatic cycles
Authors: Simonet Roda Mar , Kim Daeyeong , Brasier Alexander T. , Griesshaber Erika , Lee Jeong‐Hyun 
Published: July 2024

AbstractLacustrine stromatolites serve as important archives for recording environmental changes, and the detailed examination of their microfabrics is essential for understanding their formative processes and the environmental changes embedded within them. This study explored the application of Electron Backscatter Diffraction combined with Energy‐Dispersive X‐ray Spectroscopy to investigate a well‐preserved middle Cretaceous lacustrine stromatolite from south‐eastern Korea, unveiling ultra‐high‐resolution sedimentary processes that are often challenging to observe using conventional methods. Two types of microsparitic layers and one type of crystalline layer are distinguished based on their texture, crystal morphology and elemental composition. Both microsparitic layers are micrometre‐thick and are characterized by poorly co‐oriented calcite grains, but differ in their composition. Type 1 is depleted in magnesium but enriched in detrital elements such as silicon and aluminium, likely originating from the trapping and binding of detrital sediments on microbial mats during rainy seasons. In contrast, type 2 is enriched in magnesium but devoid of detrital elements, interpreted to have formed by the precipitation of calcium carbonate during dry seasons. The crystalline layers comprise fan‐shaped calcite crystals (ca 500 μm in length) with radiating internal structures, with their c‐axes oriented perpendicular to the stromatolite layers. These structures resemble those observed in some modern freshwater stromatolites, which are interpreted as imprints of cyanobacterial fascicules. While the cyclic occurrence of types 1 and 2 microsparitic layers might imply annual seasonal cycles, the less frequent crystalline layers are interpreted as a result of environmental changes occurring every 27 to 30 years. This is the first study to apply Electron Backscatter Diffraction to stromatolites, showcasing its potential in unravelling both the formative and diagenetic processes of ancient stromatolites.
Do firms with environmental, social, and governance reputational risk take into account board gender diversity? An analysis on a global scale
Authors: Tran Mai‐Minh‐Anh , Nguyen Ngoc‐Yen‐Chi , Quyen Nguyen‐Khanh‐Ha , Tran Phuong‐Nhu , Phan Nguyen‐Minh‐Thu , Le Anh‐Tuan 
Published: July 2024

AbstractObjectiveThis article studies whether a firm's environmental, social, and governance (ESG) reputational risk influences board gender diversity. Besides, we are also interested in the moderating role of gender equality and country development level toward the relationship between reputational risk and board gender diversity.MethodUsing a comprehensive sample of firms from 52 countries between 2007 and 2019, we employ multiple regression with fixed effects. Our findings remain robust when using alternative measures of variables and addressing endogeneity concerns by employing a two‐stage systems generalized method of moments estimation and an instrumental variable approach.ResultsCompanies with high levels of ESG reputational risk tend to add more women directors to resolve these problems. Furthermore, the empirical results point out that the positive impact of ESG reputational risk on board gender diversity is more pronounced for countries with a higher degree of gender equality or developed countries.ConclusionsOverall, our study is the first international study to link ESG reputational risk via media channels to board gender diversity.