bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Influence of Workload and Work Motivation on Employee Performance at PT Tugu Reasuransi Indonesia
Authors: Ruth Elisabet Rosemary  , Edison Siregar  , Ganda T. Hutapea  
Published: August 2024

Workloads, work motivations and employee performances can influence each other. The scope of discussion in these studies is the employee of PT. Indonesian Reinsurance Monument. Purposes of these studies was to determining (1) the effects of workloads on the performances of PT. Tugu Reasuransi Indonesia, (2) influences of work motivations on performances of employee of PT. Tugu Reasuransi Indonesia, (3) the effects of workloads and work motivation simultaneously on performances of employees of PT. Indonesian Reinsurance Monument. Quantitative method in these studies used SPSS version 25. Population of these studies was all employee of PT. Tugu Reasuransi Indonesia, which has 110 Employees use saturate sample techniques, so that data processed is 86 people. Results of these studies are as follow: (1) effects of workloads on performances of PT. The Indonesian Reinsurance Monument obtained tcount (8.582) > ttable (1.988) with a significant levels of 0.00 <0.05. From these result stated that Ho was reject (Ha accept). That show that workload affect the performances of employee of PT. Tugu Reasuransi Indonesia, (2) influence of the work motivations on performances of employee of PT. The Indonesian Reinsurance Monument obtained tcount (5.743) > ttable (1.988) with a significant level of 0.00 <0.05. From these results it can be conclud that Ho is reject (Ha is accept). This shows thats work motivation affect the performances of employee of PT. Indonesian Reinsurance Monument. (3) effect of workloads and the work motivation simultaneously on performances of employee of PT. The Indonesian Reinsurance Monument obtained fcount (36.654) > ftable (3.11) with a significant level of 0.00 <0.05. From these result stated that Ho was reject (Ha accept). That indicates are workload and the work motivation simultaneously affect performances of employee of PT. Indonesian Reassurance Monument.
Keywords: workload, work motivation, and employee performance
PENGARUH KONFLIK INTERPERSONAL ANTAR KARYAWAN TERHADAP KINERJA KARYAWAN PADA PT KEMUNING MITRA PERSADA CABANG SURABAYA III
Authors: Mutiara Hati Br Sinuraya  , Netty Laura  , RutmanLumbantoruan  
Published: August 2024

The objective of this research is to investigate how interpersonal conflicts impact employee performance within PT Kemuning Mitra Persada Surabaya III Branch. The study utilized a survey approach, employing a questionnaire as the primary tool for data collection. The target population comprised all employees of PT Kemuning Mitra Persada Surabaya III Branch, with a sample size of 80 individuals chosen through proportional random sampling. The data gathering techniques encompassed observation, interviews, questionnaires, and documentation. The collected data underwent analysis, including descriptive analysis, assessment of classical assumptions, and simple regression analysis using SPSS 29 software. The outcomes of the simple regression analysis revealed an equation: Y = 16.019 - 0.555 X, demonstrating a significant negative correlation between interpersonal conflict and employee performance. Essentially, this implies that heightened levels of interpersonal conflict lead to decreased employee performance. The partial hypothesis testing affirmed that interpersonal conflict has an individual, adverse, and statistically significant impact on employee performance. Consequently, it is advisable for PT Kemuning Mitra Persada Surabaya III Branch to conduct regular evaluations of conflict levels within the organization and comprehend their implications for employee performance. Continuous monitoring of conflict situations enables timely identification of emerging issues, facilitating prompt and appropriate action.

keywords: interpersonal conflict, employee performance
Identifikasi Molekular Bakteri Endofit dari Akar Pauh (Mangifera sumatrana Miq) dan Aktivitas Antimikrobanya
Authors: Zaunit Muthia Miranda , Verawati Verawati , Irwandi Irwandi , Elfionita Yolla Sarni 
Published: August 2024

Bakteri endofit merupakan bakteri yang mampu menghasilkan senyawa-senyawa bioaktif yang sama dengan tanaman inangnya. Salah satunya tumbuhan yang potensial adalah pauh (Mangifera sumatrana Miq.). Tujuan dari penelitian ini yaitu untuk mengisolasi dan menguji aktivitas antimikroba dari bakteri endofit dari akar pauh terhadap Staphylococcus aureus, Escherichia coli, dan Candida albicans serta melakukan identifikasi secara molekuler menggunakan gen 16S rRNA terhadap bakteri endofit yang memiliki aktivitas antimikroba terkuat. Hasil penelitian ini didapatkan 4 isolat yaitu AL1, Al2, AD1, dan AD2 yang memiliki karakteristik morfologi berupa bentuk tidak beraturan dan bulat, permukaan timbul datar, tepian nya ada yang bergelombang, bergerigi, dan berserabut, serta warna koloni rata-rata putih susu. Hasil uji aktivitas antimikroba dengan metode Kirby-Bauer terhadap Staphylococcus aureus pada isolat AL1, AD1, dan AD2 maka didapatkan zona hambat sebagai berikut: 11,10 mm, 8,60 mm, dan 8,00 mm. Uji aktivitas terhadap Escherichia coli dan Candida albicans tidak memiliki aktivitas antimikroba. Identifikasi molekuler menunjukkan bahwa isolat AL memiliki aktivitas antimikroba terbesar dan memiliki kemiripan 99,91% dengan Lysinibacillus macrolides.
The duct of von Ebner’s glands is a source of Sox10+ taste bud progenitors and susceptible to pathogen infections
Authors: Yu Wenxin , Kastriti Maria Eleni , Ishan Mohamed , Choudhary Saurav Kumar , Rashid Md Mamunur , Kramer Naomi , Do Hy Gia Truong , Wang Zhonghou , Xu Ting , Schwabe Robert F. , Ye Kaixiong , Adameyko Igor , Liu Hong-Xiang 
Published: August 2024

IntroductionWe have recently demonstrated that Sox10-expressing (Sox10+) cells give rise to mainly type-III neuronal taste bud cells that are responsible for sour and salt taste. The two tissue compartments containing Sox10+ cells in the surrounding of taste buds include the connective tissue core of taste papillae and von Ebner’s glands (vEGs) that are connected to the trench of circumvallate and foliate papillae.MethodsIn this study, we performed single cell RNA-sequencing of the epithelium of Sox10-Cre/tdT mouse circumvallate/vEG complex and used inducible Cre mouse models to map the cell lineages of vEGs and/or connective tissue (including stromal and Schwann cells).ResultsTranscriptomic analysis indicated that Sox10 expression was enriched in the cell clusters of vEG ducts that contained abundant proliferating cells, while Sox10-Cre/tdT expression was enriched in type-III taste bud cells and vEG ductal cells. In vivo lineage mapping showed that the traced cells were distributed in circumvallate taste buds concurrently with those in the vEGs, but not in the connective tissue. Moreover, multiple genes encoding pathogen receptors were enriched in the vEG ducts hosting Sox10+ cells.DiscussionOur data supports that it is the vEGs, not connective tissue core, that serve as the niche of Sox10+ taste bud progenitors. If this is also true in humans, our data indicates that vEG duct is a source of Sox10+ taste bud progenitors and susceptible to pathogen infections.
Men with a history of commercial heterosexual contact play essential roles in the transmission of HIV-1 CRF55_01B from men who have sex with men to the general population in Guangxi, China
Authors: Luo Tong , Zhang Fei , Liang Huayue , Yu Dee , Cen Ping , Zhong Shanmei , Qin Cai , Yang Yuan , Jiang Jiaxiao , Liao Yanyan , Li Mu , Zhang Rongjing , Li Zeyu , Lin Zhifeng , Ye Li , Liang Hao , Liang Bingyu 
Published: August 2024

BackgroundThere is increasing focus on HIV-1 CRF55_01B in China. However, there is limited information regarding the dissemination of CRF55_01B across different regions and populations in Guangxi. This study was performed to elucidate the evolutionary history of the introduction and dissemination of CRF55_01B in Guangxi.MethodsMolecular network and phylogenetic analyses were used to investigate the transmission characteristics of CRF55_01B in China. The analyses particularly focused on the cross-provincial spatial and temporal transmission patterns between Guangdong Province and Guangxi, as well as the transmission dynamics among different regions and populations within Guangxi.ResultsIn total, 2226 partial pol sequences of CRF55_01B strains sampled from 2007 to 2022 were collected, including 1895 (85.09%) sequences from Guangdong, 199 (8.94%) sequences from Guangxi, and 172 (7.59%) sequences from other provinces of China. Most people living with HIV in Guangxi were infected with HIV-1 through heterosexuals (52.76%). Among these, 19.10% had a history of commercial heterosexual contact (CHC) and 15.58% had a history of non-marital non-commercial heterosexual contact (NMNCHC). Overall, 1418 sequences were identified in the molecular network. Notably, the sequences from Guangdong Province were most closely linked to those from Guangxi. Phylogenetic analysis showed that CRF55_01B was first introduced from Shenzhen City to Nanning City around 2007. Subsequently, CRF55_01B established local transmission within Guangxi, with Nanning City serving as the transmission center from 2008 to 2017. After 2017, the CRF55_01B strain spread to other regions of Guangxi. Men who have sex with men (MSM) and men with a history of CHC have played a significant role in the transmission of CRF55_01B among different populations in Guangxi.ConclusionsThis study provides evidence on the transmission trajectory of CRF55_01B among different regions and populations in Guangxi. Given the bridging role of men with a history of CHC in the dissemination of CRF55_01B from MSM to the general population, it is imperative to enhance surveillance among key populations to mitigate the secondary transmission of HIV-1.
ERA-CRISPR/Cas12a system: a rapid, highly sensitive and specific assay for Mycobacterium tuberculosis
Authors: Gan Tian , Yu Jianwei , Deng Zhongliang , He Jun 
Published: August 2024

IntroductionMycobacterium tuberculosis, the causative agent of human tuberculosis, poses a significant threat to global public health and imposes a considerable burden on the economy. However, existing laboratory diagnostic methods for M. tuberculosis are time-consuming and have limited sensitivity levels.MethodsThe CRISPR/Cas system, commonly known as the “gene scissors”, demonstrates remarkable specificity and efficient signal amplification capabilities. Enzymatic recombinase amplification (ERA) was utilized to rapidly amplify trace DNA fragments at a consistent temperature without relying on thermal cyclers. By integrating of CRISPR/Cas12a with ERA, we successfully developed an ERA-CRISPR/Cas12a detection system that enables rapid identification of M. tuberculosis.ResultsThe sensitivity of the ERA-CRISPR/Cas12a fluorescence and lateral flow systems was 9 copies/μL and 90 copies/μL, respectively. Simultaneously, the detection system exhibited no cross-reactivity with various of respiratory pathogens and non-tuberculosis mycobacteria, demonstrating a specificity of 100%. The positive concordance rate between the ERA-CRISPR/Cas12a fluorescence system and commercial qPCR was 100% in 60 clinical samples. Meanwhile, the lateral flow system showed a positive concordance rate of 93.8% when compared to commercial qPCR. Both methods demonstrated a negative concordance rate of 100%, and the test results can be obtained in 50 min at the earliest.DiscussionThe ERA-CRISPR/Cas12a system offers a rapid, sensitive, and specific method that presents a novel approach to laboratory diagnosis of M. tuberculosis.
Single-center external validation and reconstruction of multiple predictive models for skip lateral lymph node metastasis in papillary thyroid carcinoma
Authors: Li Qi , Huang Can , Zou Hongrui , Zhang Jiaping , Xin Jingwei 
Published: August 2024

ObjectiveThe unique metastatic pattern of skip lateral lymph node metastasis (SLLNM) in PTC patients may lead to missed diagnosis of lateral cervical metastatic lymph nodes. Therefore, many different SLLNM prediction models were constructed. In this study, partially eligible models (Hu 2020, Wang 2020, and Zhao 2023 nomograms) were selected for external validation, and then new variables were incorporated for model reconstruction to extend clinical applicability.Methods576 PTC patients from our center were selected to evaluate the performance of the three nomograms using the receiver operating characteristic curve (ROC), calibration curves, and decision curve analyses (DCA). Three new variables were added to calibrate the model, including assessment of LN status on ultrasound (US-SLLNM), the distance from the tumor to the capsule (Capsular distance), and the number of central lymph node dissections (CLND number). Univariate and multivariate logistic regression analyses were used to screen independent predictors to reconstruct the model, and 1000 Bootstrap internal validations were performed.ResultsSLLNM were present in 69/576 patients (12.0%). In external validation, the area under the ROC curves (AUCs) for Hu 2020, Wang 2020, and Zhao 2023 nomograms were 0.695 (95% CI:0.633-0.766), 0.792 (95% CI=0.73-0.845), and 0.769 (95% CI:0.713-0.824), respectively. The calibration curves for the three models were overall poorly fitted; DCA showed some net clinical benefit. Model differentiation and net clinical benefit improved by adding three new variables. Based on multivariate analysis, female, age, and maximum tumor diameter ≤ 10 mm, located at the upper pole, Capsular distance &lt; 0mm, US-SLLNM, CLND number ≤ 5 were identified as independent predictors of SLLNM and were used to construct the new model. After 1000 Bootstrap internal validations, the mean AUC of the model was 0.870 (95% CI:0.839-0.901), the calibration curve was close to the ideal curve, and the net clinical benefit was significant.ConclusionOverall, these nomograms were well differentiated and provided some net clinical benefit, but with varying degrees of underestimation or overestimation of the actual risk and high false-negative rates. New dynamic nomogram was constructed based on the addition of new variables and larger samples, showing better performance.
Association of remnant cholesterol with sarcopenia in Korean adults: a nationwide population-based study using data from the KNHANES
Authors: Jang Soo Yeon , Hwang Soon-Young , Jang Ahreum , Kim Kyeong Jin , Yu Ji Hee , Kim Nam Hoon , Yoo Hye Jin , Kim Nan Hee , Baik Sei Hyun , Choi Kyung Mook 
Published: August 2024

BackgroundMounting evidence indicates the importance of the interplay between skeletal muscles and lipid metabolism. Remnant cholesterol (remnant-C) is considered one of the principal residual risk factors for cardiovascular disease and metabolic disorders; however, there are limited studies on the impact of remnant-C on sarcopenia.MethodsData from the Korea National Health and Nutrition Examination Surveys (KNHANES) between 2008 and 2011 were used in this nationwide population-based study. In total, 17,408 participants were enrolled in this study. The subjects were categorized into four groups according to the quartile of remnant-C values. We conducted multivariable logistic regression analysis to evaluate the association between remnant-C and muscle mass measured using dual-energy X-ray absorptiometry.ResultsA total of 1,791 participants (10.3%) presented low muscle mass, and there was a sequential increase in the percentage of low muscle mass across remnant-C quartiles (Q1, 5.2%; Q2, 8.7%; Q3, 11.5%; Q4, 15.7%). In the full adjusted model, those in the highest remnant-C quartile group showed significantly increased odds ratio (OR) for low muscle mass compared with those in the lowest remnant-C group after adjusting for various confounding factors (OR = 1.33, 95% confidence interval (CI) = 1.06–1.68, P &lt;0.05). A wide range of subgroups and sensitivity analyses showed consistent results, supporting the robustness of our findings.ConclusionsIncreased remnant-C value was associated with a high risk of low muscle mass in the Korean population. Remnant-C may be a novel marker for the prediction and management of sarcopenia in aging societies.
Does the Belt and Road Initiative reduce smog pollution in key provinces along the route?
Authors: Jiang Jingao , Wu Maoguo 
Published: August 2024

This study investigated the impact of the Belt and Road Initiative (BRI) on reducing smog pollution in key provinces along the route. Utilizing data from 284 prefecture-level cities in China from 2007 to 2018, this study adopted a propensity score matching-difference-in-differences approach, and conducted a series of robustness checks. The results indicate that the BRI has overall reduced particulate matter with aerodynamic diameter less than 2.5 µm (PM2.5) levels in key provinces along the route, and robustness checks find consistent results. Dynamic effect tests reveal a significant reduction in the annual average PM2.5 levels in key provinces along the route after the BRI was implemented in 2014. Tests on underlying mechanism find that the BRI mainly reduced PM2.5 levels in key provinces along the route by promoting technological innovation and optimizing industrial structure. Furthermore, heterogeneity tests find that the BRI significantly reduced PM2.5 levels only in cities of key provinces along the Silk Road Economic Belt, with variations in significance due to regional differences, city administrative levels, and marketization. In addition, the BRI has enhanced green total factor productivity in key provinces along the route by reducing PM2.5 levels. This study enriches research on the economic consequences of the BRI in terms of environmental protection, and also provides empirical support for the construction of the green “Belt and Road.”
Evaluation of inflammatory biomarkers and their association with anti-SARS-CoV-2 antibody titers in healthcare workers vaccinated with BNT162B2
Authors: Leno-Duran Ester , Serrano-Conde Esther , Salas-Rodríguez Ana , Salcedo-Bellido Inmaculada , Barrios-Rodríguez Rocío , Fuentes Ana , Viñuela Laura , García Federico , Requena Pilar 
Published: August 2024

IntroductionVaccine-induced immunity against COVID-19 generates antibody and lymphocyte responses. However, variability in antibody titers has been observed after vaccination, and the determinants of a better response should be studied. The main objective of this investigation was to analyze the inflammatory biomarker response induced in healthcare workers vaccinated with BNT162b2, and its association with anti-Spike (a SARS-CoV-2 antigen) antibodies measured throughout a 1-year follow-up.MethodsAnti-spike antibodies and 92 biomarkers were analyzed in serum, along with socio-demographic and clinical variables collected by interview or exploration.ResultsIn our study, four biomarkers (ADA, IL-17C, CCL25 and CD8α) increased their expression after the first vaccine dose; and 8 others (uPA, IL-18R1, EN-RAGE, CASP-8, MCP-2, TNFβ, CD5 and CXCL10) decreased their expression. Age, body mass index (BMI), smoking, alcohol consumption, and prevalent diseases were associated with some of these biomarkers. Furthermore, higher baseline levels of T-cell surface glycoprotein CD6 and hepatocyte growth factor (HGF) were associated with lower mean antibody titers at follow-up, while levels of monocyte chemotactic protein 2 (MCP-2) had a positive association with antibody levels. Age and BMI were positively related to baseline levels of MCP-2 (β=0.02, 95%CI 0.00-0.04, p=0.036) and HGF (β=0.03, 95%CI 0.00-0.06, p=0.039), respectively.ConclusionOur findings indicate that primary BNT162b2 vaccination had a positive effect on the levels of several biomarkers related to T cell function, and a negative one on some others related to cancer or inflammatory processes. In addition, a higher level of MCP-2 and lower levels of HGF and CD6 were found to be associated with higher anti-Spike antibody titer following vaccination.
Relationship between serum vitamin C and serum uric acid in people with different BMIs: results from the NHANES 2017–2018 and Mendelian randomization study
Authors: Zhang Jiajie , Jiang Hejun , Fu Guanghui , Wu Zou , Yao Yukai , Sun Jie 
Published: August 2024

ObjectiveTo examine the association of overweight/obesity and serum vitamin C (serum VC) with serum uric acid (SUA) and to assess causality using Mendelian randomization (MR).Methods4,772 participants from the National Health and Nutrition Examination Survey (NHANES), 2017–2018 were included in this study. Multivariate linear regression, variance inflation factor and quantile regression were used to analyze the relationships between overweight/obesity and serum VC and SUA levels. Secondly, Mendelian randomization (MR) was utilized to mitigate bias and prevent reverse causality in the observational study. Genetic variants associated with obesity (N = 13,848), vitamin C levels (N = 64,979) and serum uric acid levels (N = 343,836) were sourced from the most extensive genome-wide association studies (GWAS). The primary analytical method employed was inverse variance weighted (IVW).ResultsBased on the observational study, BMI was positively associated with SUA (β = 0.06, 95% CI: 0.05 to 0.07, p &lt; 0.001) and serum VC was negatively associated with SUA (β = −0.14, 95% CI: −0.23 to −0.04, p = 0.005). In individuals with overweight/obesity (BMI &gt; =25), the negative effects of serum VC on SUA enhanced with increasing serum VC. High serum VC level (Q4 level, above 1.19 mg/dL) reduced SUA (β = −0.30, 95% CI: −0.47 to −0.14, p &lt; 0.001) in individuals with overweight/obesity compared to low serum VC level (Q1 level, below 0.54 mg/dL). IVW-MR analysis revealed a significant association between SUA levels and genetically elevated levels of VC (β = −0.03, 95% CI: −0.06 to −0.00, p = 0.029) and obesity (β = 0.06, 95% CI: 0.04 to 0.07, p &lt; 0.001).ConclusionCross-sectional observational analysis revealed that BMI exhibited a positive correlation with SUA levels and that serum VC was negatively correlated with SUA levels; moreover, moderate serum VC can reduce SUA, especially in individuals with overweight/obesity. There was evidence indicating a causal effect of VC and obesity on SUA. It highlights the importance of VC in the management of SUA levels, particularly in overweight/obese individuals. The findings might be helpful for the management of high SUA levels.
Pulse crops: nutrient density, affordability, and environmental impact
Authors: Drewnowski Adam , Conrad Zach 
Published: August 2024

IntroductionSustainable foods need to be nutrient-rich, affordable, environmentally friendly, and socially acceptable. Pulses, which include beans, lentils, chickpeas, and dried peas are a food group that can fit all those criteria.MethodsThese concepts were tested serially using nutrient profiling methods that focused on protein and were extended to include food prices, greenhouse gas emissions, and energy demand. The present sustainability analyses were based on the US Department of Agriculture (USDA) nutrient composition and food prices data. Environmental impact data came from life cycle assessments (LCA). First, the USDA Protein Foods Group was disaggregated into animal and plant proteins. Plant proteins were separated into pulses, soy products, and nuts and seeds.ResultsPulses were among the lowest-cost protein sources (per 100 g and per 100 kcal) and had the lowest greenhouse gas emissions GHGE and energy demand. Pulses were among the most sustainable foods when monetary and energy costs were expressed per 50 g of protein (equivalent to 100% DV). Pulses scored well on the Nutrient Rich Food (NRF9.3) nutrient profiling system and on the related Affordable Nutrition Index that assessed nutrient density per penny.DiscussionPulses are a source of low-cost plant-based protein and a variety of priority vitamins and minerals, have low carbon footprint and energy demand, and are a valued culinary ingredient across diverse regions and cultures. As dietary guidance turns to plant-based diets, pulses need to be integrated into the global sustainability framework.