bluuebunny/crossref_metadata_2025_split huggingface dataset:
METTL3 shapes m6A epitranscriptomic landscape for successful human placentation
Authors: Kumar Ram Parikshan , Kumar Rajnish , Ganguly Avishek , Ghosh Ananya , Ray Soma , Islam Md. Rashedul , Saha Abhik , Roy Namrata , Dasgupta Purbasa , Knowles Taylor , Niloy Asef Jawad , Marsh Courtney , Paul Soumen 
Published: July 2024

AbstractMethyltransferase-like 3 (METTL3), the catalytic enzyme of methyltransferase complex for m6A methylation of RNA, is essential for mammalian development. However, the importance of METTL3 in human placentation remains largely unexplored. Here, we show that a fine balance of METTL3 function in trophoblast cells is essential for successful human placentation. Both loss-of and gain-in METTL3 functions are associated with adverse human pregnancies. A subset of recurrent pregnancy losses and preterm pregnancies are often associated with loss of METTL3 expression in trophoblast progenitors. In contrast, METTL3 is induced in pregnancies associated with fetal growth restriction (FGR). Our loss of function analyses showed that METTL3 is essential for the maintenance of human TSC self-renewal and their differentiation to extravillous trophoblast cells (EVTs). In contrast, loss of METTL3 in human TSCs promotes syncytiotrophoblast (STB) development. Global analyses of RNA m6A modification and METTL3-RNA interaction in human TSCs showed that METTL3 regulates m6A modifications on the mRNA molecules of critical trophoblast regulators, includingGATA2, GATA3, TEAD1, TEAD4, WWTR1, YAP1, TFAP2CandASCL2, and loss of METTL3 leads to depletion of mRNA molecules of these critical regulators. Importantly, conditional deletion ofMettl3in trophoblast progenitors of an early post-implantation mouse embryo also leads to arrested self-renewal. Hence, our findings indicate that METLL3 is a conserved epitranscriptomic governor in trophoblast progenitors and ensures successful placentation by regulating their self-renewal and dictating their differentiation fate.
REAL-TIME VISUALIZATION OF SPLICEOSOME ASSEMBLY REVEALS BASIC PRINCIPLES OF SPLICE SITE SELECTION
Authors: Donovan Benjamin T , Wang Bixuan , Garcia Gloria R , Mount Stephen M , Larson Daniel R 
Published: July 2024

ABSTRACTThe spliceosome is a megadalton protein-RNA complex which removes introns from pre-mRNA, yet the dynamic early assembly steps have not been structurally resolved. Specifically, how the spliceosome selects the correct 3’ splice site (3’SS) amongst highly similar non-functional sites is not known. Here, we develop a kinetic model of splice site selection based on single-molecule U2AF heterodimer imagingin vitroandin vivo. The model successfully predicts alternative splicing patterns and indicates that 3’SS selection occurs while U2AF is in complex with the spliceosome, not during initial binding. This finding indicates the spliceosome operates in a ‘partial’ kinetic proofreading regime, catalyzed in part by the helicase DDX42, which increases selectivity to the underlying U2AF binding site while still allowing for efficient forward progression.ONE-SENTENCE SUMMARYWe apply a kinetic proofreading model to elucidate how transient U2AF binding leads to high fidelity splice site selection.
Experimental reproduction of viral replication and disease in dairy calves and lactating cows inoculated with highly pathogenic avian influenza H5N1 clade 2.3.4.4b
Authors: Baker Amy L. , Arruda Bailey , Palmer Mitchell V. , Boggiatto Paola , Sarlo Davila Kaitlyn , Buckley Alexandra , Ciacci Zanella Giovana , Snyder Celeste A. , Anderson Tavis K. , Hutter Carl , Nguyen Thao-Quyen , Markin Alexey , Lantz Kristina , Posey Erin A. , Torchetti Mia Kim , Robbe-Austerman Suelee , Magstadt Drew R. , Gorden Patrick J. 
Published: July 2024

AbstractHighly pathogenic avian influenza (HPAI) H5N1 of the hemagglutinin clade 2.3.4.4b was detected in the United States in late 2021 and continues to circulate in all four North American flyways to date. In addition to impacting poultry, these HPAI viruses caused mortality events in wild bird species and wild mammals. Transmission in multiple host species raises the concern for mammalian adaptation. On March 25, 2024, HPAI H5N1 clade 2.3.4.4b was confirmed in a dairy cow in Texas in response to a multi-state investigation into milk production losses. Over one hundred positive herds were rapidly identified in Texas and eleven other U.S. states. The case description included reduced feed intake and rumen motility in lactating cows, decreased milk production, and thick yellow milk. The diagnostic investigation revealed detections of viral RNA in milk and mammary tissue with alveolar epithelial degeneration and necrosis, and positive immunoreactivity of glandular epithelium by immunohistochemistry. A single transmission event, likely from avian species to dairy cattle, followed by limited local transmission preceded the onward lateral transmission of H5N1 clade 2.3.4.4b genotype B3.13. We sought to experimentally reproduce infection with genotype B3.13 in Holstein yearling heifers and lactating cows. The heifers were inoculated by an aerosol respiratory route and the cows by an intramammary route. Clinical disease was mild in the heifers, but infection was confirmed by virus detection, lesions, and seroconversion. Clinical disease in lactating cows included decreased rumen motility, changes to milk appearance, and production losses consistent with field reports of viral mastitis. Infection was confirmed by high levels of viral RNA detected in milk, virus isolation, lesions in mammary tissue, and seroconversion. This study provides the foundation to investigate additional routes of infection, transmission, and intervention strategies.
Scalable Antigen-Antibody Binding Affinity Landscape: A Case Study with ENHERTU
Authors: Li Wei 
Published: July 2024

ABSTRACTOptimization of binding affinities for antibody-drug conjugates (ADCs) is inextricably linked to their therapeutic efficacy and specificity, where the majority of ADCs are engineered to achieve equilibrium dissociation constants (Kdvalues) in the range of 10−9to 10−10M. Yet, there is a paucity of published data delineating the optimal binding affinity or its range that ensures improved therapeutic outcomes for ADCs. This study addresses this issue by integrating structural biophysics within a scalable in silico workflow to generate antigen-antibody binding affinity landscapes, with a focus on Trastuzumab, a monoclonal antibody employed in the treatment of HER2-positive breast cancer. By leveraging high-throughput computational techniques, including homology structural modeling and structural biophysics-based Kdcalculations, this research puts forward a set of high-accuracy structural and intermolecular binding affinity data for Her2-Trastuzumab-Pertuzumab (PDB entry 6OGE). Beyond the design of Her2-targeting ADCs with enhanced efficacy and specificity, this scalable antigen-antibody binding affinity landscape also offers a technically feasible workflow for the high-throughput generation of synthetic structural and biophysical data with reasonable accuracy. Overall, in combination with artificial intelligence (e.g., deep learning) algorithms, this synthetic data approach aims to catalyze a paradigm shift in the discovery and design of antibodies and ADCs with improved efficacy and specificity.SIGNIFICANCEWith Trastuzumab as an example, this study presents a scalable computational biophysical generation of antigen-antibody binding affinity landscapes, serving two purposes: design of Her2-targeting ADCs with enhanced efficacy and specificity and continued accumulation of synthetic structural biophysics data for the development of useful AI-based drug discovery and design model in future. This scalable approach is broadly applicable to databases such as Protein Data Bank.
Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems
Authors: Gillespie William , Zhang Yuwen , Ruiz Oscar E. , Cerda Juan , Ortiz-Guzman Joshua , Turner Williamson D. , Largoza Gabrielle , Sherman Michelle , Mosser Lili E. , Fujimoto Esther , Chien Chi-Bin , Kwan Kristen M. , Arenkiel Benjamin R. , Devine W. Patrick , Wythe Joshua D. 
Published: July 2024

ABSTRACTHere we present the Multisite Assembly of Gateway Induced Clones (MAGIC) system, which harnesses site-specific recombination-based cloning via Gateway technology for rapid, modular assembly of between 1 and 3 “Entry” vector components, all into a fourth, standard high copy “Destination” plasmid backbone. The MAGIC toolkit spans a range of in vitro and in vivo uses, from directing tunable gene expression, to driving simultaneous expression of microRNAs and fluorescent reporters, to enabling site-specific recombinase-dependent gene expression. All MAGIC system components are directly compatible with existing multisite gateway Tol2 systems currently used in zebrafish, as well as existing eukaryotic cell culture expression Destination plasmids, and available mammalian lentiviral and adenoviral Destination vectors, allowing rapid cross-species experimentation. Moreover, herein we describe novel vectors with flankingpiggyBactransposon elements for stable genomic integration in vitro or in vivo when used withpiggyBactransposase. Collectively, the MAGIC system facilitates transgenesis in cultured mammalian cells, electroporated mouse and chick embryos, as well as in injected zebrafish embryos, enabling the rapid generation of innovative DNA constructs for biological research due to a shared, common plasmid platform.
A comprehensive understanding of memorable experiences in food tourism
Authors: Akhoondnejad Arman 
Published: July 2024

Purpose
More specifically, the research identifies the determinants of positive and negative memorable experiences in different contexts of food tourism.

Design/methodology/approach
Qualitative data were collected from domestic and international tourists visiting New Zealand. Using an in-person, self-administered, electronic survey, 374 complete surveys were gathered in Christchurch City.

Findings
The findings reveal 25 factors contributing to positive memorable experiences, whereas the lack of seven of them leads to negative memorable experiences. Food quality is the most dominant determinant of both positive and negative memorable experiences.

Originality/value
Despite existing various types of food and drink experiences, the literature largely focused on the consumption part. The negative memorable experience has also been neglected. To expand the knowledge of the determinants of memorable food/drink experiences, this research focuses on all types of such experiences including positive and negative ones to provide a comprehensive understanding of memorable experiences within food tourism.
Innovative 3D-printed surfaces for efficient water harvesting from air
Authors: Koyun Furkan Turan , Sabur Sema , Başal Güldemet , Günerhan Hüseyin 
Published: July 2024

PurposeThe purpose of this study is to develop nature-inspired 3D surfaces for atmospheric water harvesting.Design/methodology/approachInitially, cylindrical-shaped protrusions were produced utilizing a 3D printer to obtain a surface with a high surface area. Subsequently, an electrospraying technique was employed to coat the tips of these hydrophobic protrusions with hydrophilic nano-scale particles and fibers, utilizing polyamide 6 (PA6) or PA6/chitosan (CH) blends. In the next stage of the study, the impact of protrusion shape was investigated by fabricating surfaces with cylindrical, conical and tree-shaped protrusions. Following the production of 3D surfaces, PA6 was electrosprayed onto the protrusions to achieve varied wettability patterns on the 3D surface. Finally, the water collection rates and capacities of the surfaces were evaluated.FindingsWater collection tests demonstrated that PA6-coated surfaces exhibited greater water collection capacity compared to untreated surfaces. Furthermore, the addition of CH enhanced the water collecting efficiency of the 3D surface. It was found that the shape of the protrusions significantly influenced water collection capacity. Particularly, cone-shaped protrusions exhibited the highest water collecting capability among the different shapes tested.Originality/valueIn this study, 3D printing and electrospraying techniques were combined to create 3D surfaces characterized by high surface area, along with hydrophilic and hydrophobic regions to produce superior surfaces for atmospheric water harvesting.
Decoding the puzzle: exploring the factors influencing homeownership in Togo
Authors: Yenlide Tchablemane , Okey Mawussé Komlagan Nézan 
Published: July 2024

Purpose
This study aims to analyze the factors influencing households housing tenure choices in Togo.

Design/methodology/approach
The authors applied a rigorous econometric approach, using Harmonized Household Living Conditions Survey (EHCVM) data from 2018 and 2021 to construct a longitudinal panel, and Unified Basic Welfare Indicators Questionnaire (QUIBB) data from 2006, 2011 and 2015 to construct a pseudo-panel.

Findings
The study reveals that a household’s life-cycle variables like age of the household head, marital status, household size and place of residence, have a significant influence on homeownership. In addition, households in the highest wealth quartiles and used heads of household are more likely to own their home.

Research limitations/implications
Housing policies focused on improving the financial sustainability of low-income households and reducing the transaction costs associated with property acquisition are essential to promoting homeownership.

Practical implications
As part of the implementation of the Government Roadmap 2020–2025, the government has committed to providing 20,000 affordable social housing units, aiming to significantly boost the supply of decent housing. However, the findings of this study highlight the need for targeted subsidy programs for low-income households, particularly for female-headed households and those living in urban areas. These subsidies could cover part of the cost of purchasing homes. For middle-income households, it is crucial to develop suitable financing mechanisms, such as low-interest mortgages and loan guarantees. Given demographic pressures and the high cost of public housing programs, promoting self-build remains essential. This support should be accompanied by the provision of low-cost building materials and technical training in innovative, sustainable construction methods. Additionally, improved access to employment and land regularization are essential prerequisites for the success of these initiatives.

Originality/value
Research on the determinants of tenure choice is relatively limited in sub-Saharan Africa due to the unavailability of housing survey data. This paper proposes a case study of Togo, whose housing market characteristics correspond to most sub-Saharan African countries. Furthermore, this study applied two methodological approaches commonly used in dynamic analyses, thereby enhancing the robustness of the findings.
Effects of transformational leadership on the learning organization and nurses' innovative behavior in Vietnamese public hospitals
Authors: Nguyen Phuoc 
Published: July 2024

PurposeThe purpose of this study is to explore the effects of transformational leadership on learning organization and nurses' innovative behavior. Furthermore, this study aims to explore the mediating role of learning organization in the relationship between transformational leadership and nurse innovative behavior in the healthcare context. As there is a paucity of research examining these relationships, this study bridges the research gaps in the literature.Design/methodology/approachA cross-sectional survey was used. Using convenience sampling, the data of 228 nurses from 5 public hospitals in Vietnam were collected via questionnaires. A structural equation model (SEM) was used to empirically test the hypotheses.FindingsThe findings show significant positive effects of transformational leadership on learning organization and nurses’ innovative behavior. Furthermore, a significant positive relationship between learning organization and nurses’ innovative behavior was also supported. Finally, the partial mediating role of learning organization is significant in this study.Originality/valueTo the best of the author's knowledge, this article is the first of its kind to identify the minimal literature available on the impact of transformational leadership on learning organization and nurses’ innovative behavior. This article also contributes to extending knowledge about the mediating role of learning organization in the healthcare context. Highlighting the importance of transformational leadership, the findings offer guidance for healthcare organizations that aim to strengthen nurses’ innovative behavior.
Beyond fear and safety: revenge travel and international tourists’ intentions in the post-crisis era
Authors: Salman Ahmad , Trupp Alexander , Stephenson Marcus L. , Chan Ling Foon 
Published: July 2024

Purpose
This study aims to investigate the evolving travel intentions of tourists in the aftermath of the relaxation of international mobility restrictions in 2022. It aims to understand how the concept of “revenge travel” – travelling with the intent to make up for lost time during crisis periods – impacts tourists' travel intentions in the post-crisis era.

Design/methodology/approach
Employing a quantitative approach, the study uses Structural Equation Modelling (SEM). Data were collected through a survey of 320 respondents in 2022. This methodology enables a comprehensive understanding of travel intentions, including motivations, perceptions of destination safety and the influence of revenge travel.

Findings
The findings reveal that revenge travel is a significant predictor of travel intentions post-crisis. The results indicate that tourists are less concerned with health and safety and are more driven by the desire to compensate for lost travel opportunities. This shift suggests that travel intentions in the post-crisis era are not predominantly fear-induced. The study also offers insights into how these intentions can inform the recovery and sustainable planning of the tourism industry.

Originality/value
This paper recognises the impact of revenge travel on travel intentions following the lifting of crisis-related mobility restrictions. It provides novel insights into tourists' post-crisis travel behaviour, extending the understanding of travel motivations in extraordinary circumstances. The findings are valuable for tourism practitioners and researchers, offering guidance for future tourism development and marketing strategies within a post-crisis context.
Visualization of global research trends and future research directions of financial performance in higher education sector using bibliometric analysis
Authors: Aghel Mohamed , Azam S.M.Ferdous , Aza Azlina Md Kassim 
Published: July 2024

PurposeThe purpose of this research is to undertake a bibliometric analysis of financial performance research in of higher education sector. The study examines papers over the last 2 decades and performed performance analysis, co-citation analysis, bibliographic coupling and scientific mapping.Design/methodology/approachThe study examines 616 documents retrieved from the Scopus database using bibliometric analysis, performance analysis and thematic clustering. The study looked at the scientific productivity of papers, prolific authors, most influencing papers, institutions and nations, keyword cooccurrence, thematic mapping, co-citations and authorship and country collaborations. VOS viewer was employed as a tool in the research to conduct the performance analysis and thematic clustering.FindingsThis study delves into the recent advancements in financial performance research within higher education, focusing particularly on the year 2023, characterized by a peak of productivity with 46 significant articles. Notable institutions contributing substantially to this discourse include the University of Sussex (UK), and Ratio Institute Stockholm (Sweden), each referenced 227 times. The United Kingdom has emerged as a leader in financial performance research, amassing 3,850 citations from 92 publications. Key journals driving this conversation include “Entrepreneurship: Theory and Practice” and “The British Journal of Political Science.” The most cited study examines the impact of business-university partnerships on innovation and financial outcomes.”Originality/valueThis is the first study that provides a performance analysis and scientific mapping of the financial performance literature in the higher education sector. In addition, this study is the initial one to do a thorough analysis and organized representation of financial performance in the higher education sector, providing an unparalleled understanding of a hitherto uninvestigated area of academic research.
Academic integrity violations in higher education: a systematic literature review from 2013–2023
Authors: Sozon Md , Sia Bee Chuan , Pok Wei Fong , Alkharabsheh Omar Hamdan Mohammad 
Published: July 2024

PurposeThis study aimed to review the different types of academic integrity violations reported in the literature between 2013 and 2023. Moreover, this study investigated the causes of these violations and recommends approaches and measures that can be implemented to minimise academic integrity violations in the era of artificial intelligence (AI).Design/methodology/approachWe reviewed 27 articles published between 2013 and 2023 from the Scopus database. We followed the reporting guidelines of the Preferred Reporting Items for Systematic Reviews and Meta-Analyses to select, filter, and report relevant studies that met the research objectives.FindingsThe study revealed that various individual, institutional, social, cultural, and technological factors contribute to academic integrity violations. To minimise this issue, higher education institutions should offer brief academic integrity tutorial courses to all first-year students to enhance their knowledge of academic integrity. Moreover, academic honour codes should be regularly reviewed and updated, and technological tools should be implemented to detect AI-generated third-party assignments submitted by students. Furthermore, higher education institutions should frequently conduct training and workshops to educate students on the consequences of violating academic integrity.Originality/valueThis study contributes to existing literature. It highlights the wider societal implications of academic integrity violations such as their influence on workforce readiness and ethical standards in professional environments.