bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of a new Non-Classified Chlamydia Species in Hens in Poland
Authors: Szymańska-Czerwińska Monika , Niemczuk Krzysztof , Sachse Konrad , Mitura Agata , Karpińska Teresa Agnieszka , Reichert Michał 
Published: March 2013

Abstract
The outbreak of chlamydiosis in one of the western provinces of Poland, was diagnosed accidentally as a concurrent infection in a commercial laying hen flock during an outbreak of fowl pox. For histological examination, skin and subcutaneous tissue samples from lesions on heads of the birds were collected. Swabs from throat and trachea have been examined by nested PCR, real-time PCR, and partial ompA sequencing. Detailed electron microscopy analysis revealed fowl pox intracytoplasmic inclusions, called Bollinger bodies, and the presence of other intracytoplasmic inclusions; specific for Chlamydia sp. Results of nested PCR confirmed the presence of Chlamydiaceae sp. in two tested samples. Surprisingly, one of the two Chlamydiaceae-positive cases turned out to be infected with a non-classified strain. Results of real-time PCR and sequencing confirmed the presence of a new Chlamydia species that has not been found in Poland to date. Partial sequencing and BLAST analysis of ompA gene sequence confirmed the highest homology to non-classified poultry strains of Chlamydia sp. that were previously detected in Germany and France. The zoonotic potential and the exact taxonomic status of this atypical strain have yet to be defined.
Quantitative Changes in Selected Lymphocyte Subpopulations after Administration of a Soluble Parasitic Antigen of Babesia Canis to Dogs
Authors: Adaszek Łukasz , Lisiecka Urszula , Carbonero Martinez Alfonso , Skrzypczak Maciej , Winiarczyk Stanisław 
Published: March 2013

Abstract
Changes in selected blood lymphocyte subpopulations in dogs administered with a soluble parasitic antigen (SPA) derived from a supernatant of 18S RNA-A and 18S RNA-B Babesia canis cell culture were investigated. The studies included 20 dogs divided into three groups: group I (n=8) - comprised of dogs receiving SPA twice, at 3 week intervals; group II (n=5) - nonvaccinated control dogs, and group III (n=7) - dogs vaccinated twice with a commercial B. canis vaccine. Cytometric analysis revealed that vaccination with SPA derived from B. canis culture had similar effects to the vaccination with a commercial vaccine. The vaccination lowered the percentage of T lymphocytes (CD3+), T helper cells (CD4+), cytotoxic/supressor T cells (CD8+), B lymphocytes (CD21+), and MHC II lymphocytes in the blood in comparison to non-vaccinated dogs. Statistical analysis of the results demonstrated that mean values of the tested parameters at each stage of the study were similar in groups I and III and significantly higher in group II. The lowered level of the lymphocyte subpopulations in groups I and III persisted during the whole period of the study. The results presented that SPA has immunosuppressive effect in the first period after being administrated.
Comparative Analysis of Protein Profiles of Malassezia Pachydermatis Strains Isolated from Healthy Dogs and Dogs with Otitis Externa
Authors: Nowakiewicz Aneta , Ziółkowska Grażyna 
Published: March 2013

Abstract
The study included 40 strains of Malassezia pachydermatis obtained in pure culture from external ear canal of dogs and the reference strain. Protein fractions were received by mechanical disruption of the fungal cells. Denaturing protein electrophoresis was performed according to Laemmli method. More than 90% of the all tested strains were characterised by the presence of the following protein fractions: 83.0; 77.0; 59.0; 55.0; 48.0; 38.0; 35.0; 28.0, and 27.0 kDa. In other regions of electrophoregrams, a relatively high differentiation was observed. The strains isolated from animals with otitis externa were characterised by the presence of the following protein fractions: 75.0; 61.0; 52.0; 36.0; 19.5; 16.0; 13.8, and 13.0 kDa. These fractions were absent in the commensal strains. The use of statistical analysis confirmed the obtained results and allowed to generate dendrogram grouping potentially pathogenic and commensal strains on two different branches. Such results may indicate significant differences between protein profiles of strains of M. pachydermatis isolated from healthy and diseased animals.
Serum Concentration of Free Amino Acids in Dogs Suffering From Perianal Tumours
Authors: Brodzki Adam , Brodzki Piotr , Szpetnar Maria , Tatara Marcin R. 
Published: March 2013

Abstract
The aim of the study was to determine serum free amino acid concentration in male dogs suffering from benign and malignant perianal tumours. Serum concentrations of cysteic acid, glutamic acid, glutamine, aspartic acid, glycine, γ-aminobutyric acid, tryptophan, methionine, arginine, taurine, threonine, tyrosine, alanine, valine, isoleucine, and leucine were significantly changed in dogs suffering from benign and/or malignant tumours when compared to the control group (P<0.05). Serum concentration of serine, phenylalanine, lysine and histidine was not influenced in dogs with neoplastic disease (P>0.05). The evaluation of serum free amino acid concentration, has shown that threonine, glutamic acid, alanine, valine, isoleucine, leucine, γ-aminobutyric acid, and glycine have the highest diagnostic and prognostic value in dogs suffering from benign and malignant perianal tumours. Highly diagnostic and prognostic value in relation to benign tumour growth was also found while evaluating branched chain and acidic groups of amino acids. Serine, phenylalanine, lysine, and histidine, as well as alkaline amino acids were proved not to have diagnostic and prognostic value in dogs with the tumours. Thus, the evaluation of free amino acid concentration may serve diagnostic purposes and help in tumour malignancy differentiation. The elaborated experimental model may be used in further studies on neoplastic disease development and monitoring of applied treatment efficiency.
Prevalence of Tumours in Domestic Animals in the Lower Silesia (Poland) in 2009–2011
Authors: Ciaputa Rafał , Kandefer-Gola Małgorzata , Nowak Marcin , Madej Janusz A. 
Published: March 2013

Abstract
The study aimed at the analysis of prevalence frequency and localisation of tumours in domestic animals. The research material comprised 4,212 tumours developed in dogs, cats, horses, and exotic animals, isolated during surgery, autopsy or biopsy, performed for the purpose of histopathological diagnosis. The most numerous group involved canine tumours, including 3,585 cases (85.1%), followed by tumours in cats (532 cases, 12.6%), ferrets (34 cases, 0.81%), rats (19 cases, 0.45%), horses (15 cases, 0.36%), and rabbits (14 cases, 0.33%). A significant increase in incidence of tumours was noted, as compared to studies performed in the same region of Poland in 1957-1995 and 2000-2004 or in 2005-2008. This was particularly evident in exotic animals, in which 80 cases (1.9%) were detected in 2009-2011 but only 20 cases (1.2%) in 2005-2008. The most frequent localisation of neoplasia involved the skin, subcutaneous tissue, and mammary gland.
Effect of the Addition of Magnesium Salt to a Feed Mixture on Intestinal Microflora, Health, and Production of Sows
Authors: Trawńska Beata , Polonis Antoni , Lechowski Jerzy , Tymczyna Leszek , Borowski Ryszard , Gizińska Katarzyna 
Published: March 2013

Abstract
The effect of feed mixture supplementation with MgCl2 on alimentary tract microflora, haematological markers, and selected parameters of reproductive performance of sows was investigated. The animals were divided into control group (K) and two experimental groups (E1 and E2). The sows from the experimental groups were receiving 1 g of MgCl2 6H20/100 kg b.w./day, which constituted 120 mg of pure magnesium, administered in a small portion of a feed mixture. The sows from group E1 were receiving MgCl2 every day for 30 d, whereas sows from group E2 for 60 d. A significant decrease in the count of E. coli, Providencia sp., and Proteus sp. was noted in faeces of the swine of both experimental groups. The study has also demonstrated a reduced number of lymphocytes and an increased number of granulocytes in blood of the sows receiving MgCl2. Feeding of pregnant sows with the addition of magnesium salt decreased the mortality rate of newborn piglets, increased the survival rate of piglets until 21 d of their life, and increased body weight values of litters originating from experimental sows, compared to the litters of control sows.
Polymorphisms of Growth Hormone Gene in a Native Chicken Population: Association with Egg Production
Authors: Makhsous Samaneh Gorji , Mirhoseini Seyed Ziaeddin , Zamiri Mohammad Javad , Niazi Ali 
Published: March 2013

Abstract
A total of 142 chicken blood samples were collected and a specific primer set was used to amplify a fragment of growth hormone locus using PCR. PCR products were digested with SacI and MspI restriction endonucleases. The amplified fragment digested with SacI enzyme revealed two “+” (wild type) and “-” (normal type) alleles with the frequency of 0.898 and 0.102, respectively. The amplified fragment digested with MspI enzyme revealed three A, B and C alleles with the frequency of 0.599, 0.102, and 0.299, respectively. Frequencies of +/+, +/- and -/- were 0.817, 0.162, and 0.021, respectively, and those of AA, AB, AC, BB, BC, and CC were 0.338, 0.113, 0.409, 0.007, 0.070, and 0.063, respectively, in the studied population. The results of 2 and likelihood ratio tests showed that this population was at Hardy-Weinberg equilibrium with respect to the marker locus. Marker-trait association analysis revealed statistically significant differences between “SacI-RFLP” genotypes for egg production and rate of laying eggs. The relationship between the molecular marker and these traits can be useful to improve the chicken breeding programmes.
Physiological Values of P-Wave Dispersion in Silesian Breed Horses and Polish Primitive Horses
Authors: Michlik Katarzyna , Noszczyk-Nowak Agnieszka , Pasławska Urszula , Janus Izabela , Nicpoń Józef 
Published: March 2013

Abstract
Electrocardiographic examinations were performed on 45 Silesian breed horses (group K, 3-16-year-old, 16 stallions, 29 mares) and on 25 Polish Primitive breed horses (group P, 1.5-19-year-old, two stallions, six geldings, 17 mares). Einthoven and chest leads were used. P-wave dispersion was electronically evaluated. There was no correlation between P-wave dispersion and other ECG parameters, age, sex, body mass, or month of pregnancy. The average value of P-wave dispersion for all horses was 30.77 ms (SD 3.92 ms), however in particular groups it was: in group P - 31.89 ms (SD 3.07 ms), and in group K - 30.15 ms (SD 4.27 ms). The maximum value of P-wave dispersion in the studied population was 40 ms, in groups P and K - 37,6 ms and 40 ms, respectively. The physiological value of P-wave dispersion for the group of horses was below 45 ms.
Influence of Aflatoxin B1 on Oxidoreductive Balance in Renal Tissue of Rats
Authors: Wójtowicz-Chomicz Katarzyna , Stadnik Adam , Huk-Wieliczuk Elżbieta , Czeczuk Anna , Borzęcki Andrzej 
Published: March 2013

Abstract
The toxic effect of various doses of aflatoxin B1 on renal function was studied. Experiments were conducted on randomly chosen Wistar rats. The animals were divided into four groups. Group I received 8% alcohol intragastrically. The other groups received aflatoxin B1 in various doses. The effect of the aflatoxin on renal cells was analysed by means of determination of oxidoreductive balance and development of free radicals. The activity of antioxidative enzymes in renal tissue has decreased with an increase in the dose of aflatoxin B1. Disturbance of oxidation balance in the kidneys confirm a toxic effect of aflatoxin B1 on these organs
Tricuspid Dysplasia in Dogs
Authors: Pasławska Urszula , Noszczyk-Nowak Agnieszka , Janiszewski Adrian , Nicpoń Józef 
Published: March 2013

Abstract
The aim of the study was to estimate prevalence of tricuspid dysplasia (TD) in dogs with respect to breed, age, sex, clinical signs, and echocardiographic findings and to compare this data with literature. TD was found in 15 dogs (6.5% of congenital cardiac disease) of 215 dogs with congenital heart defects. All dogs had right heart enlargement on thoracic radiographs, echocardiography, and electrocardiography. Doppler echocardiography revealed tricuspid valve regurgitation. Seven dogs presented no clinical symptoms to date. TD took the form of Ebstein anomaly in all Labrador Retrievers, one Boxer, and one German Shepherd dog. TD predominated in males (11 males vs. three females). The body weight of the affected dogs, with the exception of the Miniature Schnauzer, exceeded 20 kg. Two dogs (Boxer and Bull Terrier) had additional congenital cardiac lesions in the form of mitral valve dysplasia. The most affected breeds in the study were the Labrador Retriever and Boxer.
Gasless Laparoscopic Ovariohysterectomy and Comparison of Serum Levels of Interleukin-6 and C-Reactive Protein after Lohe and Traditional Laparoscopic Ovariohysterectomy in Bitches
Authors: Zhang Jian-Tao , Shao Jing-Tao , Liu Yun-Feng , Wang Hong-Bin 
Published: March 2013

Abstract
Ten mixed-breed female dogs were used in the study. Abdominal wall lifting was performed with a sterilised cotton strip. Four portal sites were used to complete gasless laparoscopic ovariohysterectomy (GLOHE) procedures. The proper and suspensor ligament, ovarian pedicle, and broad ligament of the uterus, uterine artery and vein were coagulated using bipolar electrocoagulation. After the uterine body was coagulated and cut, the end of the cervix was ligated with a loop suture. Blood samples were obtained before the surgery, immediately after the procedure, and on 1, 3, and 5 d postoperatively for the determination of interleukin-6 (IL-6) and C-reactive protein (CRP). No significant surgical complications occurred. After gasless laparoscopic ovariohysterectomy, a significantly higher serum IL-6 level was found immediately after the surgery and 1 d postoperatively when compared with the level observed after traditional laparoscopic ovariohysterectomy (LOHE). However, no significant differences were observed in CRP level between GLOHE and LOHE. GLOHE proved to be a safe and feasible procedure in bitches.
Syringomyelia and Chiari Malformation in Cavalier King Charles Spaniels – Magnetic Resonance Imaging, Pharmacological and Surgical Treatment
Authors: Pomianowski Andrzej , Adamiak Zbigniew 
Published: March 2013

Abstract
Nine dogs with Chiari malformation and syringomyelia, which were subjected to low-field magnetic resonance imaging (MRI) test, were described. The results of MRI examinations were presented. The outcomes of pharmacological therapy involving two dogs and surgical treatment of one dog were also described. The applied treatments produced positive short-term outcomes, and they eliminated the clinical symptoms of the disease.
First Order Perturbation Bounds of the Discrete-Time LMI-Based H∞ Quadratic Stability Problem for Descriptor Systems
Authors: Yonchev Andrey 
Published: March 2013

Abstract
In this paper we propose an approach to obtain first order perturbation bounds for the discrete-time Linear Matrix Inequalities (LMI) based H∞ quadratic stability problem for descriptor systems. Applying the considered approach we are able to compute tight first order perturbation bounds for the LMIs’ solutions to the H∞ quadratic stability problem for discrete-time descriptor systems. In the paper we present an approach to compute the estimates of the individual condition numbers of the considered LMIs. To illustrate the performance and applicability of the results obtained we present a numerical example
Algorithm for Multiple Model Adaptive Control Based on Input-Output Plant Model
Authors: Slavov Tsonyo 
Published: March 2013

Abstract
An algorithm for multiple model adaptive control of a time-variant plant in the presence of measurement noise is proposed. This algorithm controls the plant using a bank of PID controllers designed on the base of time invariant input/output models. The control signal is formed as weighting sum of the control signals of local PID controllers. The main contribution of the paper is the objective function minimized to determine the weighting coefficients. The proposed algorithm minimizes the sum of the square general error between the model bank output and the plant output. An equation for on-line determination of the weighting coefficients is obtained. They are determined by the current value of the general error covariance matrix. The main advantage of the algorithm is that the derived general error covariance matrix equation is the same as this in the recursive least square algorithm. Thus, most of the well known RLS modifications for the tracking timevariant parameters can be directly implemented. The algorithm performance is tested by simulation. Results with both SISO and MIMO time variant plants are obtained.
Analysis of a Vector Space Model, Latent Semantic Indexing and Formal Concept Analysis for Information Retrieval
Authors: Aswani Kumar Ch. , Radvansky M. , Annapurna J. 
Published: March 2013

Abstract
Latent Semantic Indexing (LSI), a variant of classical Vector Space Model (VSM), is an Information Retrieval (IR) model that attempts to capture the latent semantic relationship between the data items. Mathematical lattices, under the framework of Formal Concept Analysis (FCA), represent conceptual hierarchies in data and retrieve the information. However, both LSI and FCA use the data represented in the form of matrices. The objective of this paper is to systematically analyze VSM, LSI and FCA for the task of IR using standard and real life datasets.
Some Applications of Intuitionistic Fuzzy Sets for the Determination of a Sociometric Index of Acceptance
Authors: Dworniczak Piotr 
Published: March 2013

Abstract
A simple use of intuitionistic fuzzy sets to determine and process the information describing the level of acceptance of people in a social group (e.g., pupils in a school class) is presented in the paper. An algorithm of processing linguistic assessments is given that leads to aggregated opinions and conclusions in the form of linguistic evaluation.
Open Access to Resource Management through Web Services
Authors: Atanasov Ivaylo , Pencheva Evelina , Marinska Dora 
Published: March 2013

Abstract
The paper investigates the capabilities for open access to resource management in multimedia networks through Parlay X Web Services. Resource management allows the network operator to provide Quality of Service (QoS) to user sessions and to apply advanced charging. The study is based on the analysis of Policy and Charging Control (PCC) functions defined for evolved packet systems The PCC comprises flow-based charging including charging control and online credit control, gating control, and Quality of Service Control (QoS) control. The required functionality for open access to QoS management and advanced charging is identified. Parlay X Web Services are evaluated for the support of PCC and some enhancements are suggested. Implementation aspects are discussed and Parlay X interfaces are mapped on IMS control protocols. Use Cases of Parlay X Web services for PCC are presented.
Site effects on root characteristics and soil protection capability of two cover crops grown in South Bulgaria
Authors: Himmelbauer Margarita L. , Vateva Violeta , Lozanova Ljudmila , Loiskandl Willibald , Rousseva Svetla 
Published: March 2013

Abstract
Water erosion has been recognized as a major soil degradation process worldwide. This is of special relevance in the semi-arid areas of South Bulgaria with long periods of drought along with severe rainfall events. The main objective of this study was to evaluate the applicability of Bromus innermis L. and Lotus corniculatus L. for soil protection purposes under different site conditions. The site parameters considered were slope, fertilization and a range of soil physical parameters. The plant parameters were canopy cover, biomass, and root morphological characteristics. The experiment includes plots without and with eleven rates of NPK fertilization on gentle (6o) and steep slopes (12o). It was observed that the effect of fertilization on shoot and root growth was stronger on the gentle than on the steep slopes. The biomass accumulation was more sensitive to N than the PK fertilizer applications. The increase of the root density with increasing fertilization rates was more pronounced for the mass than for length or surface area. A significant effect on root diameter was found only for the variants with the highest N application. Treatments with the highest root mass density on both slopes showed the greatest potential for reducing erosion.
Simulation of the influence of rainfall redistribution in spruce and beech forest on the leaching of Al and SO4 2- from forest soils
Authors: Nikodem Antonín , Kodešová Radka , Bubeníčková Libuše 
Published: March 2013

Abstract
The aim of this study was to assess the impact of different vegetation on the distribution of rainfall (due to throughfall and stemflow), water regime, and Al and SO4 2- leaching from forest soils. The water flow and Al and SO4 2- transport were modeled using HYDRUS-1D. The study was performed at two elevation transects on the Paličník and Smědava Mountain in Jizera mountains. Podzols and Cambisols were prevailing soil units in this area. It was shown that the effect of the precipitation redistribution on water regime was considerable in the beech forest, while it was almost negligible in the spruce forest. Redistribution of precipitation under trees caused runoff (in one case), increased water discharge through the soil profile bottom, reduction of water storage in the soil, and thus reduction of root water uptake. Simulated Al leaching from the soil profile was determined mainly by the initial Al content in the soil profile bottom. Leaching of SO4 2- was mainly determined by its initial content in the soil and to a lesser extent by redistributed precipitation and SO4 2- deposition.
The canopy rainfall interception in actual and potential distribution of Qinghai spruce (Picea crassifolia) forest
Authors: Xu Zhonglin , Feng Zhaodong , Zhao Chuanyan , Zheng Jianghua , Yang Jianjun , Tian Fengxia , Peng Huanhua , Wang Chao , Peng Shouzhang , Sher Hassan 
Published: March 2013

Abstract
Interception is one of the most underestimated processes in hydrological cycle in arid and semiarid regions. In Qilian Mountains of northwestern arid and semiarid China, the Qinghai spruce (Picea crassifolia) forest plays an important role in the hydrological cycle of the inland Heihe River basin. The historical disturbance of Qinghai spruce forest has resulted in various ecological problems. In order to realize the sustainable development of Heihe River basin, the Chinese government implemented restoration practices for Qinghai spruce in the past three decades. In this study, we estimated the rainfall interception in the actual and potential distribution of Qinghai spruce forest. Some of the important findings include: (1) The interception ratio of rainfall events ranged from 11-51% with a mean value of 27.02%; (2) Totally, 147 Mt of rainfall is intercepted by canopy of actual Qinghai spruce forest, in the projected potential distribution of the forest, totally 407 Mt of rainfall will be intercepted.