bluuebunny/crossref_metadata_2025_split huggingface dataset:
A high‐titer scalable Chinese hamster ovary transient expression platform for production of biotherapeutics
Authors: Gonzalez‐Rivera Juan C. , Galvan Alberto , Ryder Todd , Milman Monica , Agarwal Kitty , Kandari Lakshmi , Khetan Anurag 
Published: August 2024

AbstractTransient gene expression (TGE) in Chinese hamster ovary (CHO) cells offers a route to accelerate biologics development by delivering material weeks to months earlier than what is possible with conventional cell line development. However, low productivity, inconsistent product quality profiles, and scalability challenges have prevented its broader adoption. In this study, we develop a scalable CHO‐based TGE system achieving 1.9 g/L of monoclonal antibody in an unmodified host. We integrated continuous flow‐electroporation and alternate tangential flow (ATF) perfusion to enable an end‐to‐end closed system from N‐1 perfusion to fed‐batch 50‐L bioreactor production. Optimization of both the ATF operation for three‐in‐one application—cell growth, buffer exchange, and cell mass concentration—and the flow‐electroporation process, led to a platform for producing biotherapeutics using transiently transfected cells. We demonstrate scalability up to 50‐L bioreactor, maintaining a titer over 1 g/L. We also show comparable quality between both transiently and stably produced material, and consistency across batches. The results confirm that purity, charge variants and N‐glycan profiles are similar. Our study demonstrates the potential of CHO‐based TGE platforms to accelerate biologics process development timelines and contributes evidence supporting its feasibility for manufacturing early clinical material, aiming to strengthen endorsement for TGE's wider implementation.
Investigating the role of banks in promoting women‐owned micro and small businesses: Empirical evidence from an emerging economy
Authors: Khan Sadia Noor , Khan Tasneema , Jilhajj Khondokar 
Published: August 2024

AbstractWomen entrepreneurs of Bangladesh face strict terms and conditions, high interest rate, and collateral requirements while requesting access to bank finance. This paper investigates the role of banks in promoting micro and small businesses owned by women in Bangladesh in terms of access to bank finance and collateral requirement. Bangladeshi women entrepreneurs were chosen as there are surge in women entrepreneurs in the nation. The study used a close‐ended questionnaire to collect data based on stratified sampling and data health was ensured using the Harman's single factor indicating no presence of common method bias. The sample consists of 60 respondents of pilot study and 150 respondents surveyed by trained enumerators resulting a final sample size of 210. The data were collected based on primary sources from the entire Bangladesh represented by the eight divisions of the country namely Dhaka, Barishal, Chittagong, Khulna, Mymensingh, Rajshahi, Rangpur, and Sylhet. The data were analyzed based on the binary logistic regression model using the IBM SPSS Statistics. Empirical results indicate that women entrepreneurs can be granted bank finance only by the private commercial banks without pledging collateralized assets according to the rules of the central bank of Bangladesh provided they offer personal guarantee, social security and group guarantee and if they default on the loans, security will be used for loan repayment, and they will be deprived of further loan facilities. The findings of the study will help add value to the current literatures and the outcomes will be helpful to the concerned parties.
Identifying associations between sample characteristics, symptoms, and self‐efficacy differences in adult patients with rare tumors of the central nervous system who participated in a novel web‐based natural history study
Authors: Acquaye‐Mallory Alvina , Vera Elizabeth , Choi Anna , Wall Kathleen , Gilbert Mark R. , Armstrong Terri S. 
Published: August 2024

AbstractObjectiveHigh self‐efficacy is associated with improved self‐care and reduced symptoms in cancer patients but has not been fully interrogated in adults with central nervous system (CNS) cancers. We aimed to identify the relationship between self‐efficacy levels in managing emotions (SEMEM) and social interactions (SEMSI) by examining sample characteristics and symptom burden.MethodsSample characteristics and patient‐reported outcome (PRO) measures addressing self‐efficacy (PROMIS SEMEM & SEMSI) and symptom burden (MDASI BT or SP) were collected in a novel web‐based study of 158 adult patients diagnosed with rare CNS tumors.ResultsThe sample was predominantly female (73%), diagnosed with an ependymoma (66%), and had a median age of 45 (19–75). Low SEMEM was associated with a longer duration of symptoms before surgery (r = −0.26) and female gender (92%) among brain tumor (BT) participants and in spinal cord tumors (SCT), those with lower education (r = 0.29). Reporting low SEMSI was associated with being married (42%), lower education (r = 0.22), and a prolonged time with symptoms before surgery (r = 0.29) in those with BTs, with no associations identified in SCT. More severe mood‐related interference (including mood, enjoyment of life, and relationship with others) was associated with lower SEMEM among both locations (r = −0.61 brain, r = −0.28 spine) and SEMSI in BT participants (r = −0.54).ConclusionsLow self‐efficacy was linked to a prolonged time between symptom onset and initial surgery, education, gender, and marital status and was associated with higher mood‐related interference. Understanding characteristics associated with low self‐efficacy underscores a need for future studies to tailor interventions that enhance self‐efficacy.
Systemic administration of Resolvin D1 reduces cancer‐induced bone pain in mice: Lack of sex dependency in pain development and analgesia
Authors: Flippen Alyssa , Khasabova Iryna A. , Simone Donald A. , Khasabov Sergey G. 
Published: August 2024

AbstractAimsBone cancer produces severe pain that is treated with opioids, but serious side effects limit opioid utilization. There is therefore a need to develop effective and safe non‐opioid alternatives. The lipid mediator, Resolvin D1 (RvD1), could be a prospective candidate for cancer pain treatment. To assess RvD1 and other potential candidates, appropriate animal models that recapitulate clinical features must be used. Although several preclinical models of cancer pain have been developed, the influence of sex on the development of cancer pain and the effectiveness of RvD1 have not been studied.ResultsUsing a mouse model of fibrosarcoma growth in and around the calcaneus bone, we demonstrated that the mechanical hyperalgesia in the tumor‐bearing hind paw develops independently of sex, except that it developed a little sooner in female mice. A single intravenous injection of RvD1 (0.001–10 μg/kg) decreased hyperalgesia in both sexes with similar potency (ED50 = 0.0015 μg/kg) and efficacy. Repeated daily administration of 10 μg/kg RvD1 prolonged the analgesic effect and completely abolished hyperalgesia. This was also independent of sex.ConclusionIn this preclinical mouse model of bone cancer pain, the development of pain and the analgesic effectiveness of RvD1 are not influenced by sex.
Achalasia cardia: A case report in young female
Authors: Subedi Deepak , Parajuli Binod Raj , Bista Neha , Rauniyar Somee , Dhonju Kiran , Bhusal Santosh , Aryal Egesh , Adhikari Divas , Aryal Saurav , Karna Ayush 
Published: August 2024

Key Clinical MessageThis case emphasizes the need for early recognition and accurate diagnosis of achalasia in young adults to avoid exacerbation of the condition and misdiagnosis as GERD. Patient outcomes and quality of life are greatly enhanced by suitable diagnostic techniques, appropriate therapy, interdisciplinary care, and comprehensive patient education along with frequent follow‐ups.AbstractAchalasia results from the degeneration of inhibitory ganglion cells within the esophageal myenteric plexus and the lower esophageal sphincter (LES), leading to a loss of inhibitory neurons and resulting in the absence of peristalsis with failure of LES relaxation. Its origins are multifactorial, potentially involving infections, autoimmune responses, and genetics, with equal incidence in males and females. The hallmark symptoms include progressive dysphagia for solids and liquids, along with regurgitation, heartburn, and non‐cardiac chest pain. A 22‐year‐old female patient initially diagnosed with gastroesophageal reflux disease (GERD) received proton pump inhibitors and antacid gel for persistent dysphagia and regurgitation. Subsequent tests including barium esophagogram and manometry indicated Type II Achalasia Cardia. The patient showed clinical improvement with relief of dysphagia, regurgitation, and heartburn symptoms after pneumatic balloon dilatation (PBD). She was advised to follow up after 6 months with upper gastrointestinal (UGI) endoscopy and manometry in the outpatient clinic for regular endoscopic surveillance as there is a risk of transformation to esophageal carcinoma. Diagnosing achalasia in young adults poses challenges due to its diverse presentation and resemblance to other esophageal disorders like GERD. Diagnosis relies on clinical symptoms and imaging studies such as barium esophagogram revealing a bird's beak appearance and esophageal manometry showing absent peristalsis. UGI endoscopy is needed to rule out malignancy. Treatment options include non‐surgical approaches like medication and Botox injections, as well as surgical methods such as pneumatic balloon dilation, laparoscopic Heller myotomy, and per‐oral endoscopic myotomy (POEM). The treatment options depend upon the patient's condition at presentation and their individual choices. This case report emphasizes that it is crucial to consider achalasia as a potential differential diagnosis in young adults with dysphagia, especially if conventional treatments for acid peptic disorder do not alleviate symptoms. Prompt diagnosis and appropriate management can lead to significant clinical improvement and better patient outcomes.
A provably secure authenticated key agreement protocol for industrial sensor network system
Authors: Thakur Garima , Obaidat Mohammad S. , Sharma Piyush , Prajapat Sunil , Kumar Pankaj 
Published: August 2024

SummaryThe convergence of reliable and self‐organizing characteristics of Wireless Sensor Networks (WSNs) and the IoT has increased the utilization of WSN in different scenarios such as healthcare, industrial units, battlefield monitoring and so forth, yet has also led to significant security risks in their deployment. So, several researchers are developing efficient authentication frameworks with various security and privacy characteristics for WSNs. Subsequently, we review and examine a recently proposed robust key management protocol for an industrial sensor network system. However, their work is incompetent to proffer expedient security and is susceptible to several security attacks. We demonstrate their vulnerabilities against man‐in‐the‐middle attacks, privileged insider attacks, secret key leakage attacks, user, gateway, and sensor node impersonation attacks, and offline password‐guessing attacks. We further highlight the design flaw of no session key agreement in Itoo et al. Therefore to alleviate the existing security issues, we devise an improved key agreement and mutual authentication framework. Our protocol outperforms Itoo et al.'s drawbacks, as demonstrated by the comprehensive security proof performed using the real‐or‐random (ROR) model and the formal verification accomplished using the Automated Validation of Internet Security Protocols (AVISPA) tool.
Exploring the Effects of Sensorimotor Training and Relaxation Therapy on Postural Control, Balance, Sleep, and Stress in Sedentary Young Adults: Rationale, Design, and Methodology
Authors: Tanwar Tarushi , Aldabbas Mosab , Iram Iram , Veqar Zubia 
Published: August 2024

AbstractPostural control (PC) and sleep are critical in several aspects of health. Poor sleep negatively influences PC and balance, which is necessary for performing various tasks, from reaching to mobility. Moreover, sleep disturbances and consequent PC and balance deterioration are associated with job accidents, traffic accidents, falls, and injuries. Healthy adults who have inadequate sleep show a decline in optimal functioning, even in the absence of medical illnesses. This suggests that getting enough sleep, both in duration and quality, is essential to maintain optimal health. Moreover, inadequate sleep has also been observed to have a bidirectional relationship with stress levels. However, there is insufficient evidence regarding the impact of non‐pharmacological treatments to improve PC, sleep, and stress in the sedentary young adult (YA) population. This article describes the protocol for a study to investigate the effects of sensorimotor training and relaxation therapy on various static and dynamic PC tests, balance measures, and subjective and objective indices of sleep and stress among sedentary YAs with impaired sleep quality. The protocol is also designed to evaluate the effect of these therapies on fatigue, salivary cortisol levels, anxiety, and depression. Methods for assessing the sleep architecture, static and dynamic PC, balance, and stress are described along with the methods of scoring with the primary goal of providing a standardized set of assessment and scoring procedures according to the latest guidelines and gold‐standard techniques and measures that can be used reliably at different laboratories. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Postural control assessmentBasic Protocol 2: Balance assessmentBasic Protocol 3: Sleep architecture assessmentBasic Protocol 4: Salivary cortisol analysis
Enhanced analysis of equine plasma for the presence of recombinant human erythropoietin — Implementation of an improved workflow
Authors: Richards Stacey , Palmer David , Cawley Adam , Wainscott Martin , Keledjian John 
Published: August 2024

AbstractAn improved screening workflow and a robust capillary flow LC–MS confirmatory method for the detection of recombinant human erythropoietin (rHuEPO) has been implemented to increase the sensitivity of rHuEPO detection and to reduce the number of suspect samples committed to confirmatory testing. The influence of repeated dosing of epoetin‐β on the detection window of rHuEPO in equine plasma was assessed using the optimised method.Samples were initially assessed using an economical R&D Human EPO Duo‐Set ELISA Development System. Samples indicating a result greater than the batch baseline were analysed using the complementary R&D Human EPO Quantikine IVD ELISA kit. All samples recording an abnormal screening result were subjected to confirmatory analysis. Confirmation of rHuEPO in plasma (≥2.5 ml) in the range of 4–13 mIU/ml (n = 6) was achieved using immunoaffinity enrichment, tryptic digestion, and capillary flow LC–MS/MS.Four horses were administered a single dose of epoetin‐β (10,000 IU) via the subcutaneous and intravenous routes, on two occasions, seven days apart. The excretion profile was rapid with epoetin‐β detection times of 48 to 72 h following each administration, with no appreciable difference observed between the two routes of administration. This workflow has been shown as an effective anti‐doping strategy related to rHuEPO misuse and supports the use of out‐of‐competition testing of horses in the 2 to 3‐day period prior to race‐day.
Estimating changes in streamflow attributable to wildfire in multiple watersheds using a semi‐distributed watershed model
Authors: Wells Ryan , Mankin Kyle R. , Niemann Jeffrey D. , Kipka Holm , Green Timothy R. , Barnard David M. 
Published: August 2024

AbstractMore than half of water supply in the western United States is sourced from forested lands that are increasingly under wildfire risk. Studies have begun to isolate the effects of wildfire on streamflow, but they have typically used coarse temporal resolutions that cannot account for the numerous, interconnected watershed processes that control the responses to rainfall events. In this study, we employed a method to isolate fine‐scale (daily) effects of fire. Wildfire effects were estimated as the difference between measured post‐fire streamflow and unburned scenarios of post‐fire streamflow simulated by a hydrologic model calibrated to pre‐fire conditions. The method was applied to track hydrologic recovery after wildfires in six burned watersheds across the western United States: North Eagle Creek, NM (2012 Little Bear Fire), Lopez Creek, CA (1985 Las Pilitas Fire), City Creek, Devil Canyon Creek, East Twin Creek, and Plunge Creek, CA (2003 Old Fire). All six watersheds experienced prolonged increases of post‐fire streamflow, with the most consistent changes occurring during periods of low streamflow. Following 6 years of increased streamflow, Lopez Creek experienced 6 years of reduced streamflow before returning to the pre‐fire hydrologic regime. North Eagle Creek and the four watersheds affected by the Old Fire continued to have elevated streamflow 9 and 18 years post‐fire, respectively, without returning to the pre‐fire hydrologic regime.
Seismic response of column‐supported silos considering granular–structure interaction
Authors: Chen Jia , Ding Yonggang , Xu Qikeng , Liu Qiang , Cheng Xuansheng 
Published: August 2024

AbstractTo predict the seismic response of column‐supported silos (CSSs), the granular–structure interaction (GSI) analysis method is proposed with considering the combined effect of the friction between the particles–particles and the particles–silo wall. Using free‐body dynamic equilibrium equations, we reconstruct the mutual interactions between different grain portions and between the grains and the silo wall to develop the ideal calculation model of the CSS structure. Based on the analysis model, additional dynamic overpressure and the effective mass caused by the stored content interacting with the silo wall is obtained with different slenderness ratios and peak accelerations. The additional bending moment caused by the friction between the particles and silo wall is further quantified. To verify the reliability of the proposed method, we discuss some applicative examples by comparing the GSI method with other theories, Eurocode 8, and experimental results. Moreover, the along‐the‐height acceleration profiles of the silo wall and the ensiled content are analyzed according to the shaking‐table tests. The results show that the GSI method can match Janssen's theory well in the case of static pressure at slenderness ratios exceeding 1.0. The overpressure profiles along the height of the silo wall follow a nonlinear distribution, different from Eurocode 8. The bending moment obtained by predictive formulas agrees well with the experimental results for the CSS, indicating that the GSI method is reasonable. Some design and construction recommendations, including the maximum overpressure position, the reference range of the dynamic overpressure coefficient, and the reduction factors of the ensiled content mass, are proposed to facilitate the engineering applications of CSSs, considering different slenderness ratios.
Optimizing hybrid beamforming in millimeter‐wave massive multiple‐input multiple‐output systems: A gradient projection approach
Authors: Momen‐Tayefeh Mehrdad , Olfat Ali 
Published: August 2024

AbstractMillimeter waves (mmWave) present an enticing opportunity for wireless communication due to their substantial bandwidth. However, mitigating signal losses within this spectrum necessitates employing numerous antennas for transmission and reception. In practical scenarios, dedicating individual RF chains to each antenna is often unfeasible. In response to this limitation, our research investigates a hybrid beamforming approach, seeking to optimize spectral efficiency through an alternating optimization (AO) technique. Our goal is to develop an algorithm that can be easily integrated into diverse hybrid beamforming configurations. On the other hand the pursuit of optimizing spectral efficiency while adhering to the constraints imposed by phase shifters results in a non‐convex problem. To confront this challenge, we employ a gradient descent framework combined with projection methods. We introduce the gradient prediction method (GPM), which leads to a closed‐form solution for projection. Simulations underscore that this hybrid beamforming structure can achieve the performance of a fully digital beamforming method when the number of RF chains is twice the total number of data streams, regardless of the number of antennas involved. Furthermore, we will conduct a comparative performance analysis of our proposed algorithm against other established benchmark algorithms to ascertain its superiority.
Recent strategies to improve the electroactivity of metal–organic frameworks for advanced electrocatalysis
Authors: Wei Runzhi , Liu Xiaoyu , Ibragimov Aziz Bakhtiyarovich , Gao Junkuo 
Published: August 2024

AbstractMetal–organic frameworks (MOFs) have emerged as promising materials in the realm of electrocatalysis due to their high surface area, tunable porosity, and versatile chemical functionality. However, their practical application has been hampered by inherent limitations such as low electrical conductivity and a limited number of active metal sites. Researchers addressed these challenges through various strategies, including enhancing conductivity by incorporating conductive materials and metal nanoparticles, modifying the structure and composition of MOFs by replacing metal nodes and functionalizing linkers, and preparing MOFs catalysts through thermal processes such as decarburization and conversion into metal oxides, phosphides (MPs), and sulfides (MSs). This review provided a comprehensive summary of the strategies that were employed to enhance the electroactivity of MOFs for improved electrocatalytic performance in recent years. It also explored future directions and potential innovations in the design and synthesis of MOF‐based electrocatalysts, offering valuable insights for advancing their application in sustainable energy technologies.
Morphological characterization and distribution of antennal sensilla of Spodoptera frugiperda (Lepidoptera: Noctuidae) using scanning electron microscopy
Authors: Li Yong‐Ping , Zhou Hai‐Yan , Yang Yang , Ye Hui , Haack Robert A. , Cao Jun 
Published: August 2024

AbstractThe fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is a globally significant agricultural pest, causing severe damage to corn production in China. Chemical odor‐based trapping is a major approach for FAW control, making it essential to understand the FAW antennal sensillum types to enhance development of effective chemical odor attractants. In this study, we comprehensively examined the antennal sensilla types of FAW, identifying eight types and two subtypes, including Böhm's bristles, sensilla trichoidea, sensilla chaetica (I and II), sensilla coeloconica, sensilla styloconica, sensilla squamiformia (I and II), sensilla auricillica, and sensilla basiconica. Sensilla chaetica II, and sensilla squamiformia II are reported for the first time for FAW in this study. Detailed low‐voltage field emission scanning electron microscope (LVSEM) images and descriptions are provided for each sensillum type. This study provides the morphological information to aid in conducting antennal sensillum neurophysiological tests on FAW.Research Highlights
The types of sensilla of fall armyworm were examined, identifying eight types and two subtypes, including Böhm's bristles, sensilla trichoidea, sensilla chaetica (I and II), sensilla coeloconica, sensilla styloconica, sensilla squamiformia (I and II), sensilla auricillica, and sensilla basiconica.
Detailed low‐voltage field emission scanning electron microscope images and descriptions were provided for each sensillum type.