bluuebunny/crossref_metadata_2025_split huggingface dataset:
ANTIBODY TITER AND CLINICAL COURSE OF PEMPHIGUS
Authors: Imamura Sadao , Takigawa Masahiro , Higuchi Takako , Yamada Mizuho , Yoshinaga Hanako , Yanase Keiko , Yonezawa Ikuo , Harada Tetsuo , Ieki Akira , Takeda Toshio , Aoshima Toshiyuki , Soh Yoshiro , Doi Akira , Ogo Noriko , Aoshima Tadahiro 
Published: April 2015

ABSTRACTThe relationship between the titer of pemphigus antibody and the clinical course of the disease was investigated in 15 patients with various types of pemphigus.Although antibody titers generally ran parallel to fluctuations of the clinical course, the elevation of antibody titer appeared to follow the re‐appearance or exacerbation of the lesions on 10 occasions in 6 patients, while it appeared to precede the latter only on 2 occasions in 2 patients. In addition, the disappearance of the antibody was considerably delayed after disappearance of skin lesions in 2 cases. These findings suggest that the pemphigus antibody is the result of skin damage, rather than the cause of pemphigus itself.
TISSUE FIBRINOLYTIC ACTIVITY OF ALLERGIC CONTACT DERMATITIS
Authors: Shigemi Fumio , Kitamura Yukikazu , Ishimura Junya , Takeda Katsuyuki 
Published: April 2015

ABSTRACTOne of our co‐workers was sensitized with a 1% DNCB solution in acetone. Biopsies were obtained from skin lesions 1, 2, 4, 8, 12, 24, 30, 36 and 48 hours after a challenge with 0.1% DNCB solution. In the upper dermis, a rapid decrease of tissue fibrinolytic activity was found which disappeared after 30 hours. In the lower dermis, biphasic changes were observed. Tissue fibrinolytic activity increased one hour and 12–36 hours after challenge, whereas at 48 hours, when erythema was most intensive, it decreased in comparison to the normal activity.Erythema and histological changes of the epidermis and the dermis appeared after approximately 12 hours. As for lymphocytes, T‐cells were seen at first. Furthermore, B‐cells were noted after approximately 30 hours when marked infiltration began to appear.
ERYTHROPOIETIC PROTOPORPHYRIA
Authors: Ozasa Shozaburo , Yamamoto Shoso , Maeda Motomichi , Inada Shuichi , Yanai Shoko , Takagaki Kenji 
Published: April 2015

ABSTRACTThree cases of erythropoietic protoporphyria are reviewed. The three patients constitute members of one family. The protoporphyrin content of the red blood cells was high, but porphyrins and their precursors in the urine and faeces were not excessive. Other normal members of the family did not reveal high protoporphyrin content in the red blood cells. Clinical symptoms were itching, swelling, shallow depressed scars and waxy yellow discoloration on the face and brown pigmentation and thickness on the back of the hands after exposure to the sun. The microscopic findings from skin biopsy specimens of the lesions resembled changes of the lipoid proteinosis.
IMMUNOLOGICAL ASPECTS OF STAPHYLOCOCCAL SKIN INFECTION
Authors: Tsuda Shingo 
Published: April 2015

ABSTRACTPatients with staphylococcal infection of the skin were examined for immunological status. All cases examined in the present study had γ‐globulin levels within the normal range. The proliferative response of peripheral blood lymphocytes to staphylococcal antigen was significantly depressed in patients with furunculosis as compared with that in patients with common furuncle or in healthy controls. All patients with furunculosis showed higher titers of anti‐Staphylococcus aureus antibody than did those with furuncle. The dissociation between cellular and humoral immunity in furunculosis seems to be explained in terms of immune deviation.
NEVUS CELLS OBSERVED IN THE SEBACEOUS GLANDS
Authors: Shitara Atsuyuki , Sato Yoshio , Morohashi Masaaki 
Published: April 2015

ABSTRACTOf sebaceous gland‐containing specimens of melanocytic nevi, a total of 79 specimens from 70 cases of nevocellular nevus (consisting of 10 cases of compound nevus and 60 of intradermal nevus), four cases of juvenile melanoma and three of blue nevus were examined light‐microscopically in detail for the presence or absence of nevus cells or dermal melanocytes in the sebaceous glands. As a result, clusters of cells that appeared to be nevus cells were found in the sebaceous glands of specimens from a total of three cases, that is, two cases of nevocellular nevus i.e. compound nevus, and one of the cases of juvenile melanoma. Two cases were infantile and one case was the age of puberty. The origin of the nevus cells found in the sebaceous glands is discussed.
ERYTHROKERATODERMIA VARIABILIS
Authors: Maekawa Yoshihiro , Yasaka Shoko 
Published: April 2015

ABSTRACTThe case of a 10 year‐old Japanese boy with erythrokeratodermia variabilis is reported in addition to a review of the Japanese literature. The patient was first examined in our clinic on October 21, 1965, because of generalized hyperkeratotic lesions and erythematous lesions which had persisted since he was 3 months old. Hyperkeratotic and verrucous lesions were noted on his auricles and trunk, on which there were also sharply demarcated erythematous lesions of various sizes and shapes which were not elevated from the adjacent skin. Laboratory findings were within normal limits. Histopathological examination revealed a remarkable hyperkeratosis with a basket weave appearance, moderate acanthosis and a slightly thickened granular layer. Polyethylenglycol 400 and corticosteroid ointment were slightly beneficial to the hyperkeratotic lesions.
COMPOSITION AND MECHANISM OF DEPOSITION OF THE INTERCELLULAR SUBSTANCE IN PARAKERATOTIC PSORIATIC EPIDERMIS
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTIn order to elucidate both the mechanism of deposition and the composition of the substance that is observed mainly the intercellular spaces (ICS) and to a lesser extent in the cytoplasm of psoriatic epidermis, five cases of psoriasis vulgaris were examined using conventional histology and histochemistry, electron microscopy, and direct immunofluorescence technique with or without incubation in acid citrate buffer or digestion by fibrinolysin. The substance was found by electron microscopy to be rather dense and finely granular, and that in the parakeratotic horny layer was diastase‐resistant PAS positive, and showed positive immunofluorescence for immunoglobulins, complement, and fibrinogen. The fluorescence was not significantly altered by preincubation of the tissue sections in acid citrate buffer, but was significantly diminished by predigestion of tissue sections with fibrinolysin. From these findings, we concluded that the substance is likely to consist of and be derived from the components of plasma protein, and to play a role in the formation of the parakeratotic psoriatic horny layer. It also seems that the deposition of this substance is rather the result of the coagulation of plasma protein than that of antigen‐antibody reactions.
EFFECT OF ESTROGEN ON THE CONNECTIVE TISSUE OF SKIN IN RATS
Authors: Arata Jirò , Yamamoto Yasuo , Hagiyama Masaharu , Nohara Nozomi 
Published: April 2015

ABSTRACTThe influences of estrogen on body weight, skin weight, hydroxyproline and mucopolysaccharide content of skin of rats and 14C‐glucose or 14C‐glucosamine incorporation into mucopolysaccharide were studied. Estrogen repressed body weight gain both in males and females. Both wet and dry defatted skin weights were repressed by estrogen. Hydroxyproline and mucopolysaccharide content per unit weight of skin was not significantly altered by estrogen but the content calculated per unit area of skin was repressed by the hormone. No significant differential effect on the incorporation of 14C‐glucose or 14C‐glucosamine was observed.
A GRANULAR CELL TUMOR
Authors: Tamaki Kunihiko , Ishibashi Yasumasa , Kukita Atsushi 
Published: April 2015

ABSTRACTLight and electron microscopic studies of a case of granular cell tumor are reported. They showed these tumor cells should be considered to be of nervous origin. The electron microscopic study revealed a large number of membrane‐bound vacuoles within the tumor cells. It is suggested that some of them are derived from mitochondria.
CLINICAL SIGNIFICANCE OF DISCOID LESIONS IN SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) WITH PARTICULAR REFERENCE TO MALE PATIENTS WITH SLE
Authors: Hamaguchi Tsuguo , Hitaka Yoshiko , Takeuchi Ryuji , Uchida Ikuyo 
Published: April 2015

ABSTRACTA comparative study was made between an SLE patient group with a higher incidence of discoid lesions (65%) at our dermatologic clinic (Group A) and one with a lower incidence of discoid lesions (36.3%) in the report of the Epidemiology Committee of SLE from the Ministry of Health and Welfare of Japan (Group B). A higher incidence of neuropsychic symptoms was noticed in Group A than in Group B, a fact considered to reflect the lower incidence of severe renal deseases. Male patients with SLE showed discoid lesions in 100% of cases and a lower incidence of positive LE‐cell tests (33%); concomitantly, they showed frequent neuropsychic symptoms (50%) and a relatively high mortality.In the group of SLE patients with discoid lesions, a higher incidence of abnormal capillary nail fold features was noted than in the group without discoid lesions.
ELECTROPHORETIC CHARACTERIZATION OF TYROSINASE IN THE COATED VESICLES OF MALIGNANT MELANOMA
Authors: Mojamdar Manoj , Imokawa Genji , Mishima Yutaka 
Published: April 2015

ABSTRACTSince the activation and condensation of tyrosinase has been found to be carried out in the anastomosing, cysternal and tubular portion of GERL (4–6) rather than the Golgi apparatus, the tyrosinase transfer system from GERL to premelanosomes remains now to be elucidated in initial melanogenesis. Tyrosinase‐rich coated vesicles of Greene's malignant melanoma are isolated by differential ultracentrifugation and DEAE‐sephadex coloumn chromatography. Multiple forms of tyrosinase present in the isolated coated vesicles has been studied in comparison to those present in the soluble fraction. The soluble fraction contains two soluble forms in addition to the membrane bound form of tyrosinase. On ther hand, one soluble form and the membrane bound form of tyrosinase separated from the coated vesicle fraction. The significance of these findings and the possible role of coated vesicles in tyrosinase transport system is discussed.
GENERALIZED DISCOID LUPUS ERYTHEMATOSUS WITH BENIGN MONOCLONAL GAMMOPATHY
Authors: Ueki Hiroaki 
Published: April 2015

ABSTRACTThis paper presents the case of a patient with generalized discoid lupus erythematosus. During the past 25 years the patient has developed moderate systemic symptoms including leukopenia, anemia, elevated erythrocyte sedimentation rate and decreased serum complement. In the last 7 years, the serum γ‐globulin level has become conspicuously elevated, revealing a sharp monoclonal pattern. A M‐bow was detected in the IgG‐ and K‐chain precipitin arcs in immunoelectrophoresis. IgG type monoclonal gammopathy was confirmed, while the serum IgM level was decreased.No symptom suggesting the presence of multiple myeloma has ever been demonstrated by repeated bone X‐ray and the bone‐marrow examinations. The monoclonal gammopathy was well correlated with the intensity of cutaneous deteriolation and general symptoms.
ELECTROPHORETIC PROFILE OF TYROSINASE TREATED WITH NEURAMINIDASE IN VARIOUS SUBCELLULAR FRACTIONS FROM MOUSE MELANOMA*
Authors: Tomita Yasushi , Moro Shinran , Seiji Makoto 
Published: April 2015

ABSTRACTWhen various subcellular particles of Harding‐Passey mouse melanoma including rough and smooth surface membranes, the Golgi rich fraction and the melanosome fraction were subjected to polyacrylamide gel electrophoresis, the electrophoretic profiles of tyrosinase [EC 1. 14. 18. 1] in all these particles varied depending on the particles, but they became almost identical after the various cell particles were treated with neuraminidase [EC 3. 2. 1. 18]. This appears to suggest that tyrosinases which occur not only in melanosomes and the Golgi apparatus but also in rough and smooth surface membranes possess sugars containing sialic acid residues that can be removed by neuraminidase.
A CASE OF LICHEN AMYLOIDOSUS WITH RIEHL'S MELANOSIS‐LIKE LESION ON THE FACE
Authors: Masu Shin‐Ichi , Sato Akihiko , Seiji Makoto 
Published: April 2015

ABSTRACTUltrastructural studies were carried out on the patient who developed lichen amyloidosus and Riehl's melanosis‐like lesion on the legs and face, respectively. In the skin specimens from the face, there were many masses of aggregations of wavy or net‐like filaments which appeared to correspond to Civatte bodies as recognized under the light microscope at the dermo‐epidermal junction. Sometimes, an other kind of filament mass was present in the papilla. These were aggregations of straight and non‐branching filaments and were very similar to so‐called amyloid masses. These amyloid‐like masses, which seemed to be in a developmental stage, consisted of net‐like filaments as well as straight and non‐branching filaments. Therefore, the net‐like filament masses or Civatte bodies seemed to serve a particular role in the formation of these amyloid‐like filament masses. On the other hand, in the skin specimens from the leg, there were many amyloid masses in the papillae and few Civatte bodies in the dermo‐epidermal junction, but no features suggesting that Civatte bodies may serve a role in the formation of amyloid. From these observations, it may be concluded that Civatte bodies will contribute to the formation of amyloid under certain conditions like Riehl's melanosis; however, it is still uncertain whether Civatte bodies also contribute to the formation of amyloid filaments of lichen amyloidosus.
THE MORPHOLOGY AND TIME COURSE OF IMMUNE COMPLEXES INJECTED INTO THE SKIN OF RABBITS
Authors: Ueki Hiroaki , Kubo Miyoko , Nakaye Masako , Aoshima Tadahiro , Masuda Toshiki 
Published: April 2015

ABSTRACTThe morphology in in vitro prepared immune complexes (I.C.) using horseradish peroxidase as antigen and their behavior in the skin of rabbits were observed under the light microscope. Both in vitro and in vivo, solubilized I.C. were demonstrated as fine granular substances, while insoluble I.C. were shown to be lumpy or large granular aggregates. Solubilized I.C. injected into the skin disappeared quickly from the skin without being accompanied by the infiltration of polymorphonuclear leucocytes (PMN). Insoluble I.C. stayed in the skin longer and were phagocytosed by infiltrated mononuclear cells and PMN. Macroscopically, the former caused only slight skin edema, but the latter caused severe edema and erythema followed by induration, and also, occasionally, by necrosis.
DERMAL GLYCOSAMINOGLYCAN CONCENTRATION THROUGHOUT HAIR GROWTH CYCLE OF RATS
Authors: Maekawa Yoshihiro 
Published: April 2015

ABSTRACTThe dermal glycosaminoglycan (GAG) content of rats was measured quantitatively after plucking hair from the backs of the animals, which reinitiates their hair cycle. Mast cells present around the hair follicles were counted at each stage of the hair cycle and compared with the amount of GAG in the skin.GAG reached its peak on the 6th day of the anagen stage and decreased during the catagen stage. In the telogen stage, it was about the same as it had been in the catagen stage. The same pattern was noted for the number of mast cells. The 0.5 M fraction in Dowex‐1 column chromatography proved to be hyaluronic acid and its concentration throughout the hair growth cycle showed strong parallels to that of total dermal GAG.
CLINICAL SIGNIFICANCE AND CORRELATION OF ANTINUCLEAR ANTIBODIES AND ANTI‐DNA ANTIBODIES
Authors: Deng Jau‐Shyong , Chang Jeng‐Yue , Lü Yau‐Chin 
Published: April 2015

ABSTRACTThe clinical significance and correlation of antinuclear antibodies (ANA) and anti‐DNA antibodies was studied using 142 ANA positive sera from different patients having various diseases. High titers of ANA were found briefly in systemic lupus erythematosus and sometimes in scleroderma or mixed connective tissue disease. The peripheral pattern of ANA was seen exclusively in systemic lupus erythematosus and occasionally in mixed connective tissue disease. Anti‐DNA antibodies could be found in systemic lupus erythematosus, discoid lupus erythematosus, scleroderma, chronic active hepatitis, but a high titer of anti‐DNA (over 60 unit/ml) was present only in patients with systemic lupus erythematosus, especially those having lupus nephritis. There was little correlation between ANA and anti‐DNA antibodies.
SCANNING ELECTRON MICROSCOPIC STUDY OF THE HUMAN AXILLARY APOCRINE GLANDS
Authors: Inoue Taeko 
Published: April 2015

ABSTRACTThe human axillary apocrine glands were observed by scanning electron microscopy.

The axillary apocrine glands revealed three types of apocrine secretion: macroapocine, microapocrine and intermediate apocrine.In the macroapocrine type of secretion, a round projection, which lacked microvilli bulged up from the surface of the secretory cell. In the microapocrine type, the tips of microvilli which covered the large apocrine protrude were expanded and separated. In the intermediate apocrine type, several secretory protrubrances were formed at the luminal portion of the cell. Each protrubrance was composed of several swollen microvilli united.
Small round pores were observed on the luminal plasma membrane. They seemed to represent the state of eccrine secretion, but nothing definite was learned about their nature.
Some secretory cells had the luminal plasma membrane ruptured. They might not indicated a state of holocrine secretion, but were assumed to exhibit a deciduous phenomenon in the metabolism of secretory cells.
The cracked surfaces of secretory cells were observed to confirm the presence of nuclei and cell organelles.
A CASE OF EPITHELIOID SARCOMA
Authors: Akiyama Tomio , Honda Tetsuzo , Hori Makoto , Yoshida Hikotaro 
Published: April 2015

ABSTRACTA case of a 58‐year‐old man with epithelioid sarcoma is reported.Small nodular lesions, up to 10×10 mm in diameter, were present on the scalp, face and upper arm. On chest X‐ray examination, the nodular lesions were noticed in the right upper and middle lung field.Biopsy specimen of a scalp nodule and lung lesion showed granulomatous changes composed of irregular‐shaped eosinophilic cells with nuclear atypism, spindle‐shaped fibroblast‐like cells and a few multinucleated giant cells.Electron microscopic examination revealed that two types of cells, dark cells and light cells, were present in the dermal lesion. The cytoplasm of the dark cell contained a large amount of dilated rough‐surfaced endoplasmic reticulum and well‐developed protocollagen. This finding suggests that the dark cell tends to differentiate towards a fibroblast. Most of light cells had some underdeveloped rough‐surfaced endoplasmic reticulum but no filamentous elements. A few exceptional light cells have considerable rough‐surfaced endoplasmic reticulum. It therefore seems that the clear cell has a potencial for differentiation towards a histiocyte.These findings suggest that epithelioid sarcoma cells derive from undifferentiated mesenchymal cells.
A CASE OF CRYPTOCOCCAL SUBCUTANEOUS ABSCESS CAUSED BY PRIMARY OSSEOUS CRYPTOCOCCOSIS
Authors: Takizawa Kiyohiro , Nakagawa Hidemi , Ohhara Kuniaki 
Published: April 2015

ABSTRACTA patient with a cutaneous manifestation of primary osseous cryptococcosis is described. Statistically 35 cases of cutaneous cryptococcosis excluding this case and one case of primary osseous cryptococcosis without any skin manifestations were reported in Japan between 1955 and 1977. Of these 36 cases, metastatic osseous lesions were observed in only two cases. Each of them was disseminated cryptococcosis, however, the examination of osteolytic lesions was not shown. Therefore, to our knowledge, this is probably the first Japanese report of a case with a cutaneous lesion following to primary osseous cryptococcosis.
ORGAN CULTURE OF ADULT HUMAN SKIN
Authors: Yasuno Hirokazu , Maeda Motoaki , Sato Michiko , Nishimura Akifumi , Shimizu Chika , Miyamoto Yumiko , Sotomatsu Shigetaro 
Published: April 2015

ABSTRACTProliferation and differentiation of the epidermis in organ culture of adult human skin by the sponge matrix method were studied histologically and autoradiographically, and the following results were obtained:

On the first day of culture, mitotic figures were already observable in the epidermis. The outgrowth of epidermal cells at the margins of the explants started. On the second day, there was transformation to a zone that will be referred to as the newly formed stratum corneum in the upper epidermis.
On the third and fourth days, the increased growth of epidermal cells caused thickening of the epidermis. Simultaneously, Malpighian cells progressively differentiated into a cornified layer.
On and after the fifth day, the basospinous cell layer was reduced in thickness in most of explants. On the ninth and tenth days, DNA synthesis in the basal layer was still obvious, although the epidermis showed a thickness of only one or two cells overlaid with a large number of horny layers.
In the culture medium supplemented with corticosteroid, the epidermal growth was slightly depressed with lessened formation of stratum corneum in the early stages of culture as compared with the explants cultured in the basic medium. The reduction of the basospinous layer was scarcely notable after the fifth day. Even after 10–11 days, epidermal cells were well preserved and their stratified squamous architecture was less disorganized. It seemed that corticosteroid could prolong the survival of adult human skin in vitro.
These findings indicate that this culture technique could be used as a model for organ culture of adult human skin.
IMMUNE COMPLEX‐BEARING POLYMORPHONUCLEAR LEUKOCYTES IN PERIPHERAL BLOOD OF RABBITS WITH ACTIVE ARTHUS REACTIONS
Authors: Ueki Hiroaki , Nakaye Masako , Masuda Toshiki 
Published: April 2015

ABSTRACTImmune complex (I.C.)‐bearing polymorphonuclear leukocytes (PMN) were examined in the peripheral blood of rabbits in which active Arthus reactions had been induced by using horseradish peroxidase as an antigen. The I.C.‐bearing PMN's appeared in the circulation 1 or 2 hours after the induction of the Arthus reactions, decreased in number gradually and generally disappeared within 72 hours. Most of these I.Cs were formed in antibody‐excess. It may be speculated that I.C. is formed in the skin and transported into the systemic circulation or that free antigens escaping from the skin might bind directly with the corresponding antibody in the circulation and form I.Cs, which were subsequently phagocytosed by circulating PMN's. I.C.‐bearing PMN's in the peripheral blood may reflect in part immune deposits in the local tissues, which have occurred in the recent past (within 1 to 3 days). In conclusion, Arthus‐type reactions should be taken into account as an important source of circulating I.C. or I.C.‐bearing PMNs.
ECCRINE POROMA
Authors: Aoki Kiyoko , Baba Shunichi , Nohara Tadashi , Suzuki Hiroyuki 
Published: April 2015

ABSTRACTA case of eccrine poroma situated on the inner surface of the right heel of a Japanese patient was studied using histochemistry and electron microscopy.Histochemically, a strong positive reaction for Phosphorylase was observed in the lower part of the tumor. In contrast, glycogen granules were distributed in large numbers in the upper part. It is suggested that, although the tumor cells were morphologically similar, they differ from the point of view of glycogen metabolism.On electron microscopic examination, the characteristic features were exaggerated intercellular spaces. If this marked increase was a by‐product of the fixation process, it seems to depend upon the nature of the tumor cell itself.Clinical and pathological reports of eccrine poroma were reviewed to compare cases in Japan with those in other countries. The cases of eccrine poroma in both Japan and other countries showed similar characteristics. In particular, the cases studied resembled each other in age, sex and anatomical distribution, and also presented similar clinical features. Under light and electron microscopic examination, no particular differences could be detected.