bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of pollen morphology and viability of sweet and sour cherry genotypes by multivariate analysis
Authors: Rakonjac Vera , Nikolić Dragan , Čolić Slavica , Glišić Ivana , Đorđević Milena , Popovska Melpomena , Radičević Sanja 
Published: August 2024

AbstractThe aim of this study was to examine the dimensions and surface morphology of pollen grains of some sweet and sour cherry genotypes through scanning electron microscopy (SEM) as an additional alternative identification tool. In vitro pollen germination and pollen tube length as indicators of their viability were determined as well. Observations were carried on 10 sweet cherry (Prunus avium L.) and 7 sour cherry (Prunus cerasus L.) genotypes. All genotypes had prolate, tricolpate pollen grains, and striate exine ornamentation, with more parallel longitudinal ridges. Significant differences among genotypes within species and between species were found for most pollen grain characteristics and exine ornamentation. In both sweet and sour cherry the largest variability was recorded for colpus length (CV = 15.0% and 12.9%, respectively). For sweet cherry genotypes, in vitro pollen germination and pollen tube length ranged between 1.4% to 51.5% and 360.4 to 669.3 μm respectively, while for sour cherries they ranged from 15.5% to 37.0% and 96.3 to 960.2 μm, respectively. The results of the correlation analysis showed that in vitro pollen germination correlated positively with pollen length/pollen width (L/W) ratio (r = .640) and furrow width (r = .588), and negatively with the number of ridges (r = −.517), while pollen tube length was not significantly correlated with any of the studied characteristics. Principal component analysis (PCA) revealed that pollen length, pollen width, L/W ratio, colpus length, mesocolpium width, and ridge width are relevant tools to discriminate among the studied genotypes. The measurements made on pollen grains did not reveal individually big differences, but when all features were considered together, the pollen of each genotype exhibited a unique pattern. The distribution on the scatter plot showed considerable variation among sweet and sour cherry genotypes based on pollen morphological characteristics, which led to their distribution into two separate groups. This demonstrates the ability to distinguish cherry species based on pollen morphological characteristics determined by SEM. To improve discriminative ability for genotypes within species combination between pollen ultrastructural analysis, morphological and molecular markers is desirable, in subsequent work.Research Highlights
Significant differences in pollen characteristics between sweet and sour cherry.
Significance of pollen morphology in taxonomic differentiation.
Significance of SEM studies for the taxonomic identification.
Steatosis, inflammation, fibroprogression, and cirrhosis in remnant liver post‐liver donation
Authors: Jacob Jeby , Joseph Amal , Nair Harikumar R , Prasad Geevarghese Prajob , Kumar Vijosh V , Padmakumari Lekshmi Thattamuriyil 
Published: August 2024

AbstractBackground and AimThis is a cross‐sectional observational study conducted on living liver donors focusing on “long‐term remnant liver health” specifically looking at steatosis, inflammation, and fibrosis using multiparametric ultra sonological evaluation and noninvasive blood tests.MethodsMultiparametric ultrasound evaluation included assessment of shear wave elastography (fibrosis), sound speed plane wave ultrasound, attenuation plane wave ultrasound (steatosis), and viscosity plane wave ultrasound (inflammation). Blood test based APRI and FIB‐4 were calculated. Liver biopsy was performed if noninvasive evaluation pointed toward clinically relevant fibro progression (F4).ResultsOut of 36 donors, significant fibrosis (>F2) was found in 11 donors (30.5%), seven donors (19.4%) had severe fibrosis (>F3), and two donors had shear wave elastography values suggestive of cirrhosis(F4). Of these two, one donor was extensively evaluated and was found to have biopsy proven cirrhosis with endoscopic evidence of portal hypertension. The prevalence of fatty liver disease in our study group was 50%.ConclusionWe report the first liver donor cohort with fibroprogression and cirrhosis occurring in the remnant liver. Our donor cohort with a significant proportion having steatosis and fibroprogression underscores the importance of regular follow‐up of liver donors and evaluation of remnant liver.
Natural disease course of chronic visceral acid sphingomyelinase deficiency in adults: A first step toward treatment criteria
Authors: Eskes Eline C. B. , van Dussen Laura , Brands Marion M. M. G. , Vaz Frédéric M. , Aerts Johannes M. F. G. , van Kuilenburg André B. P. , Sjouke Barbara , Hollak Carla E. M. 
Published: August 2024

AbstractAcid sphingomyelinase deficiency (ASMD) is an ultra‐rare lysosomal storage disease with a broad spectrum of manifestations ranging from severe neuropathic forms to attenuated, chronic visceral forms. Manifestations of the chronic visceral subtype are variable and encompass different degrees of hepatosplenomegaly, pulmonary disease and dyslipidemia. The aim of this study was to provide insights into the natural course of adult patients with the chronic visceral subtype. Based on these insights, we proposed tentative criteria for initiation and follow‐up of enzyme replacement therapy (ERT). The data of 23 adult patients were collected in a prospective study. Clinical, genetic and demographic data, plasma measurements, abdominal imaging, pulmonary imaging, pulmonary function tests and quality of life questionnaires were collected. Stability of disease based on several clinical, biochemical and radiological markers (i.e., spleen volume, platelet levels, liver volume, alanine aminotransferase [ALT] levels, diffusion capacity of the lungs for carbon monoxide [DLCO] chitotriosidase activity and lysosphingomyelin [LSM]) was assessed. Cardiovascular risk was estimated based on sex, age, smoking, systolic blood pressure and lipid profile. Quality of life was evaluated with the 36‐Item Short Form Health Survey and the Health Assessment Questionnaire. Median follow‐up was 6.1 years (range 1.3–19.5 years). The most common manifestations were splenomegaly (100%), decreased high‐density lipoprotein cholesterol (HDL‐C) plasma levels (83%), (signs of) steatosis measured with transient elastography (82%), thrombocytopenia (64%), hepatomegaly (52%) and decreased diffusion capacity (45%). The majority of markers remained stable during follow‐up. Twelve patients showed progression of disease: four for spleen volume, two for liver volume, three for DLCO, seven for chitotriosidase activity and three for LSM. One patient showed progression of disease based on four markers, although this patient did not report any problems at the last visit. Cardiovascular risk was estimated and was increased in half of the patients older than 40 years. Patient‐reported quality of life did not differ from the general population, but differences in median 36‐Item Short Form Health Survey (SF‐36) scores of patients with severe pulmonary involvement and those of patients without pulmonary involvement were observed. Tentative criteria for initiation and effect of therapy were proposed. In conclusion, the chronic visceral subtype of ASMD showed a predominantly stable disease course in this cohort. We propose that ERT should be initiated on an individual basis and only in case of progression or symptomatic disease. Collection and analysis of real world data are necessary to refine start, stop and follow‐up criteria in the future.
“Having our say” revisited: Wellness of Black women counselors
Authors: Erby Adrienne N. , Jones Connie T. , DeCree Shekyra 
Published: August 2024

AbstractAs a subset of a larger mixed‐methods study of Black counselor wellness, a sequential explanatory research design was used to explore the relationship between the Strong Black Woman (SBW) schema and wellness in Black women counselors. Regression results indicated the SBW schema was a statistically significant predictor of wellness. Grounded in critical Black feminist theory, an interpretative phenomenological analysis of focus group data identified five themes of wellness and coping for Black women counselors.
Ethnic identity, negative media portrayal, and psychological well‐being in Arab‐American youth: Mediation analysis
Authors: Dari Tahani , Saleem Muniba , Nassar Sylvia , Abou‐Dahech Hala 
Published: August 2024

AbstractResearchers have increasingly identified a growing concern in the lack of attention given to factors intertwined with the mental health and well‐being of Arab‐American youth. To circumvent this problem, we applied a community‐based participatory research (CBPR) design to a sample of Arab‐American youth with the mean age of 13.72 years. We discuss the implications for professional counseling and other mental health interventions with Arab‐American youth, as well as provide directions for future research.
Associations Between Brain Metabolites Measured With MR Spectroscopy and Head Impacts in High School American Football Athletes
Authors: Liu Zexuan , Dudley Jonathan A. , Diekfuss Jed A. , Ahmed Nadine , Edmondson Alex D. , Cecil Kim M. , Yuan Weihong , Zuleger Taylor M. , Slutsky‐Ganesh Alexis B. , Barber Foss Kim D. , Myer Gregory D. , Fleischer Candace C. 
Published: August 2024

BackgroundWhile changes in brain metabolites after injury have been reported, relationships between metabolite changes and head impacts are less characterized.PurposeTo investigate alterations in neurochemistry in high school athletes as a function of head impacts, concussion, and the use of a jugular vein compression (JVC) collar.Study TypeProspective controlled trial.SubjectsA total of 284 male American football players, divided into JVC collar and noncollar groups; 215 included in final analysis (age = 15.9 ± 1.0 years; 114 in collar group).Field Strength/Sequence3 Tesla/T1‐weighted gradient echo, 1H point resolved spectroscopy, acquired between August and November 2018.AssessmentHead impacts were quantified using accelerometers. Concussion was diagnosed by medical professionals for each team. Pre‐ to postseason differences in total N‐acetylaspartate (tNAA), total choline (tCho), myo‐inositol (myoI), and glutamate + glutamine (Glx), in primary motor cortex (M1) and anterior cingulate cortex (ACC), relative to total creatine (tCr), were determined.Statistical TestsGroup‐wise comparisons were performed using Wilcoxon signed‐rank, Friedman's, and Mann–Whitney U tests. Relationships between ∆metabolite/tCr and mean g‐force were analyzed using linear regressions accounting for concussion and JVC collar. Significance was set at P ≤ 0.05.ResultsIn participants without concussion, a significant decrease in tCho/tCr (0.233 ± 1.40 × 10−3 to 0.227 ± 1.47 × 10−7) and increase in Glx/tCr (1.60 ± 8.75 × 10−3 to 1.63 ± 1.08 × 10−2) in ACC were observed pre‐ to postseason. The relationship between ∆tCho/tCr in M1 and ACC and mean g‐force from >80 g to >140 g differed significantly between participants with and without concussion (M1 β ranged from 3.9 × 10−3 to 2.1 × 10−3; ACC β ranged from 2.7 × 10−3 to 2.1 × 10−3). Posthoc analyses revealed increased tCho/tCr in M1 was positively associated with mean g‐force >100 g (β = 3.6 × 10−3) and >110 g (β = 2.9 × 10−3) in participants with concussion. Significant associations between in ACC and mean g‐force >110 g (β = −1.1 × 10−3) and >120 g (β = −1.1 × 10−3) were observed in the collar group only.Data ConclusionDiagnosed concussion and the use of a JVC collar result in distinct neurochemical trends after repeated head impacts.Level of Evidence2Technical EfficacyStage 3
Assessing the impact of temperature, pH, light and chemical oxidation on fucoxanthin colour changes, antioxidant activity and the resulting metabolites
Authors: Yusof Zuhaili , Lim Vuanghao , Khong Nicholas Mun Hoe , Choo Wee Sim , Foo Su Chern 
Published: August 2024

AbstractBACKGROUNDThe present study evaluated the effects of temperature, pH, light and chemical oxidation on fucoxanthin changes in terms of colour, antioxidant activity and metabolomic profile. Additionally, the correlation between antioxidant activity and identified metabolites was analysed.RESULTSIt was found that colour change was significantly reduced at elevated heat (100 °C, *∆E = 0.81 ± 0.05), reduced pH (pH 3, *∆E = 0.59 ± 0.04) and length of light exposure (*∆E = 3.16 ± 0.04). Antioxidant activity decreased under all treatments. Among the temperatures tested, fucoxanthin exhibited the highest activity at 60 °C, ranging from 0.92 to 3.04 mg Trolox equivalents (TE) g−1. Significant activity reductions (P < 0.05) were observed as a result of pH changes in the 2,2‐diphenyl‐1‐picrylhydrazyl and β‐carotene bleaching assays. Exposure to light 2: warm white lamp for 120 h significantly reduced antioxidant activity (0.01 to 1.70 mg TE g−1). Chemical oxidation also led to reduced activity, ranging from 0.18 to 0.29 mg TE g−1. Multivariate data analysis revealed distinct profiles for temperature, pH, light and chemical oxidation treatments. Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS)‐based metabolomics analysis identified 10 metabolites, and significant correlations (P < 0.05) indicate that these metabolites contributed to the samples' antioxidant activities.CONCLUSIONIn conclusion, fucoxanthin tolerates well at 60 °C, within pH range 3–9, and within 8 h of light exposure, as indicated by its consistent antioxidant activity and minimal colour change. Each treatment resulted in distinct metabolite concentrations, as shown by LC‐MS/MS‐based metabolomics analysis. Further research into these metabolites could advance the understanding of their roles and aid in optimising processing conditions to favour beneficial metabolites. © 2024 The Author(s). Journal of the Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
The revised Clinician‐Administered PTSD scale for DSM‐5 (CAPS‐5‐R): Initial psychometric evaluation in a trauma‐exposed community sample
Authors: Jackson Brianna N. , Weathers Frank W. , Jeffirs Stephanie M. , Preston Thomas J. , Brydon Cassidy M. 
Published: August 2024

AbstractThe Clinician‐Administered PTSD Scale for DSM‐5 (CAPS‐5) is a widely used, well‐validated structured interview for posttraumatic stress disorder (PTSD). It was recently revised to improve various aspects of administration and scoring. We conducted a psychometric evaluation of the revised version, known as the CAPS‐5‐R. Participants were 73 community residents with mixed trauma exposure (e.g., sexual assault, physical assault, transportation accident, the unnatural death of a loved one). CAPS‐5‐R PTSD diagnosis demonstrated good test–retest reliability, кs = .73–.79; excellent interrater reliability, кs = .86–.93; and good‐to‐excellent alternate forms reliability with the CAPS‐5, кs = .79–.93. In addition, the CAPS‐5‐R total PTSD severity score demonstrated excellent test–retest reliability, intraclass correlation coefficient (ICC) = .86; interrater reliability, ICC = .98; and alternate forms reliability with the CAPS‐5, r = .95. Further, the CAPS‐5‐R demonstrated good convergent validity with other measures of PTSD and good discriminant validity with measures of other constructs (e.g., depression, anxiety, alcohol problems, somatic concerns, mania). Given its strong psychometric performance in this study, as well as its improvements in administration and scoring, the CAPS‐5‐R appears to be a valuable update of the current CAPS‐5.
Surgical Outcomes in Patients with Endolymphatic Sac Tumors: A Single‐Center Experience of 29 Patients
Authors: Yang Yuanyuan , Li Feitian , Ma Mengye , Dai Chunfu 
Published: August 2024

ObjectiveTo analyze the clinical features and surgical outcomes of patients with endolymphatic sac tumors (ELSTs).Study DesignSingle institution retrospective cohort study.MethodsThe clinical data of 29 patients with 30 ELSTs who underwent surgery were retrospectively reviewed. Information on patient demographics, tumor size and grade, intraoperative blood loss volume, hearing and facial nerve outcomes, and follow‐up data was collected and analyzed.ResultsThe main symptoms were hearing loss in 26 ELSTs (86.7%) and tinnitus in 17 (56.7%). Twenty‐four (80%) ELSTs were in advanced stages (Grade III). The median tumor volume was 6.35 cm3. The median intraoperative blood loss volume was 300 mL. Facial nerve function was well preserved in 21 patients. Among all patients with Grade III tumors, 12 patients underwent tension‐free anterior facial nerve rerouting, and 11 patients (91.7%) maintained good facial nerve function postoperatively (HB I and HB II). Only one patient exhibited permanent vocal cord paralysis, and no patients experienced cerebrospinal fluid (CSF) leakage postoperatively. Gross total resection was achieved in 22 patients (73.3%), 5 patients (16.7%) experienced tumor recurrence, and 3 (10%) had residual tumors.ConclusionsMost ELSTs tend to be diagnosed in the advanced stage. Tension‐free anterior facial nerve rerouting could maximally preserve facial nerve function. The intraoperative blood loss volume was associated with tumor size and stage. Tumor recurrence tends to occur at the posterior edge of the petrosal bone, internal auditory canal, and surface of the posterior fossa. Given the relatively high recurrence rate of ELSTs, long‐term follow‐up is recommended.Level of Evidence4 Laryngoscope, 2024
Nanoliter‐Scale Light–Matter Interaction in a Fiber‐Tip Cavity Enables Sensitive Photothermal Gas Detection
Authors: Yan Yue , Xiao Xunzhou , Nie Qinxue , Wang Zhen , Chen Yifan , Wu Jiahao , Zhou Nansen , Zhou Renjie , Yang Sen , Ren Wei 
Published: August 2024

AbstractLaser spectroscopy offers a significant tool for revealing specific molecular details with the desired accuracy and sensitivity. However, it poses challenges to maintain high sensitivity when targeting a micro‐region. Here, a dual‐enhanced photothermal approach is presented using a high‐finesse fiber Fabry–Pérot (F–P) cavity, tailored for highly sensitive chemical sensing with nanoliter‐scale light–matter interaction. A spheric surface (diameter: 50 µm, radius of curvature: 910 µm) is created on the fiber tip using focused ion beam milling. By adding a high‐reflectivity dielectric coating to the spheric surface, a fiber F–P cavity is obtained with a length of 473 µm and a finesse exceeding 4000. The intra‐cavity pump light within the gas‐filled fiber cavity generates a strong photothermal effect upon gas absorption. This effect induces phase modulation, which is amplified and detected by coupling a probe laser to the fiber cavity‐based interferometer. A minimum detection limit of 10 parts‐per‐billion (ppb) of C2H2 at 1530.37 nm is demonstrated using only 1 mW of pump power, corresponding to a normalized noise equivalent absorption coefficient of 9.1×10−11 cm−1∙W∙Hz−1/2. This platform breaks the bottleneck of ultrasensitive gas detection with a very short light–matter interaction length, promising significant advancements in microscale chemical analysis through optical investigations.
Optimal extended warranty strategy considering channel perception and endogenous quality
Authors: Zhang Keang , Zhang Tao , Gao Shang 
Published: August 2024

AbstractExtended warranties (EWs) are common in online sales, available from various providers. Manufacturers are typically perceived as better repairers, making their EWs more valuable than retailers'. We explore four cases: no provider (Model N), manufacturer‐provided (Model M), retailer‐provided (Model R), and dual‐provider (Model B). Initially, we analyze exogenous quality, then consider endogenous quality with variable failure rates (Models VN, VM, VR, and VB). While product demand is consistent, EW demand varies across models. Dual‐channel EWs (Model B/VB) maintain manufacturer EW demand but reduce retailer EW demand. They increase profits under exogenous quality but may not under endogenous quality.
In utero morphological and functional properties of bovine trophoblastic vesicles
Authors: Kagawa Shinjiro , Hayashi Yoshihiro , Bai Hanako , Takahashi Masashi , Kawahara Manabu 
Published: August 2024

AbstractIn many mammals, including ruminants, pregnancy requires pregnancy recognition signaling molecules secreted by the conceptus; however, the mechanism underlying pregnancy establishment in cattle remains unknown. Trophoblastic vesicles (TVs) are artificially produced from the extraembryonic tissues of the elongating conceptus and may be useful tools for understanding conception. This study investigated the morphological and functional properties of TVs in comparison to those of intact conceptuses. TVs were prepared from the extraembryonic tissues of conceptuses collected 14 days after artificial insemination (AI), cryopreserved immediately after dissection, and cultured after thawing for subsequent transplantation into the uterus. The transferred TVs were collected 7 days after transplantation and compared with extraembryonic tissue samples collected from conceptuses at 21 days post‐AI. The recovered TVs were 40 times longer than those of their pre‐transplant counterparts. Microscopic evaluation revealed that their membrane structures consisted of trophoblast and hypoblast layers. The expression patterns of the cell differentiation markers, CDX2, SOX2, and GATA6, and interferon tau (IFNT) protein expression levels in the TVs were similar to those in control extraembryonic tissue samples. These findings suggest that TVs are capable of morphological elongation and maintain IFNT production in a similar way as original trophoblasts.
Some remarks on Lode angle and Lode parameter
Authors: Bruhns Otto T. 
Published: August 2024

AbstractIn the literature of plasticity, the variables Lode angle and Lode parameter are increasingly used as coordinates to describe the stress space when defining suitable yield or limit conditions ‐ often, however, without any or only alleged reference to the original work of Walter Lode. The present study will attempt to clarify this situation and provide an appraisal and classification of Lode's work.