bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measurement of Eyeball Protrusion and Its Application in Facial Reconstruction
Authors: Wilkinson CM , Mautner SA 
Published: August 2015

Abstract
The estimation of eyeball protrusion from the bony orbit has traditionally relied upon Wolff's theory (1976) that the cornea will be tangental to a line taken from the superior to the inferior orbital margin. This study tested this theory by taking measurements from MRI cranial images of 78 eyes of white adult subjects. Orbital depth had a significant negative linear correlation with eyeball protrusion, and protrusion of the eyeball was determined by the following standard: eyeball protrusion = 18.3 — (0.4 x orbit depth). Current eyeball position determination was shown to be as much as 3.9 mm too deep in the socket, and a practical application of this result to facial reconstruction is discussed.
Characterization of a Novel Dimorphism in the 5‵ Flanking Region of the Short Tandem Repeat (STR) Locus, c-fes/fps (FES)
Authors: Bieschke ET , Wallace MM , De Forest PR , Shaler RC , Prinz M 
Published: August 2015

Abstract
The FES short tandem repeat (STR) locus contains seven to 14 repeats of the tetranucleotide sequence ATTT. A novel 10 base pair dimorphism in the 5' flanking region of the FES locus was characterized in four broad populations: African-American, Hispanic, Caucasian, and Asian. The absence of the 10 base pair sequence, or (−) allele, was closely linked to FES STR alleles with 10 or fewer repeats. The presence of the 10 base pair sequence, or (+) allele, was closely linked to FES STR alleles with 12 or more repeats. The (−) and (+) alleles occurred equally often in FES STR allele11. The nucleotide sequence (5'-GGCTGTTTTG-3') of the (+) allele, located 179 base pairs upstream of the FES STR, was determined to be consistent within and among the four populations. Statistical and sequence analysis confirmed the linkage between the two polymorphic sites. The results indicate that the exclusion rate of the FES locus is increased, above that for the STR alone, when both polymorphic characteristics are considered.
GC-MS of Ignitable Liquids Using Solvent-Desorbed SPME for Automated Analysis
Authors: Harris AC , Wheeler JF 
Published: August 2015

Abstract
Solid-phase microextraction (SPME) is well documented with respect to its convenience and applicability to sampling volatiles. Nonetheless, fire debris analysts have yet to widely adopt SPME as a viable extraction technique, although several fire debris studies have demonstrated the utility of SPME coupled with gas chromatography-mass spectrometry (GC-MS) to identify ignitable liquids. This work considers the expansion of SPME sampling from the customary thermal desorption mode to solvent-based analyte desorption for the analysis of ignitable residues. SPME extraction fibers are desorbed in 30μL of nonaqueous solvent to yield a solution amenable to conventional GC-MS analysis with standard autosampler apparatus. This approach retains the advantages of convenience and sampling time associated with thermal desorption while simultaneously improving the flexibility and throughput of the method. Based on sampling results for three ignitable liquids (gasoline, kerosene, and diesel fuel) in direct comparisons with the widely used activated charcoal strip (ACS) method this methodology appears to be a viable alternative to the routinely used ACS method.
Development and Validation of a Multiplexed Y-Chromosome STR Genotyping System, Y-PLEX™6, for Forensic Casework
Authors: Sinha SK , Budowle B , Arcot SS , Richey SL , Chakraborty R , Jones MD , Wojtkiewicz PW , Schoenbauer DA , Gross AM , Sinha SK , Shewale JG 
Published: August 2015

Abstract
A Y-chromosome multiplex polymerase chain reaction (PCR) amplification kit, known as Y-PLEX™6, has been developed for use in human identification. The Y-PLEX™6 kit enables simultaneous amplification of six polymorphic short tandem repeat (STR) loci located on the non-recombinant region of the human Y-chromosome. These loci are: DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385. Our studies show that as little as 0.2 ng of template DNA can be used for analysis. The specificity of the amplification reaction enabled analysis of male DNA in a male:female DNA mixture at a ratio of 1:125. Among the six Y-STR loci, the maximum mean stutter percentage was 11.9 for allele at DYS389II locus. Attempts at amplification of DNA from various animal sources revealed that the Y-PLEX™6 primers are human specific. Details of the development of the kit, generation and description of the allelic ladders, and validation of the multiplex PCR are presented. In addition, Y-STR allele and haplotype frequencies in three populations have been investigated. The data indicate that results obtained using the Y-PLEX™6 kit are robust, sensitive, and reliable and can be used in human forensic and male lineage identification cases. Erratum to this paper appears in 48(3).
Iatrogenic Catheter-Related Cardiac Tamponade: A Case Report of Fatal Hydropericardium Following Subcutaneous Implantation of a Chemotherapeutic Injection Port
Authors: Shields LBE , Hunsaker DM , Hunsaker JC 
Published: August 2015

Abstract
The need to obtain dependable access to the vascular system constitutes a significant component in the treatment and management of critically ill patients. Intravenous chemotherapy administered to cancer patients over an extended period of time often results in loss of peripheral vascular access due to vein sclerosis, “exhaustion” or tissue necrosis. Medical investigators have designed and steadily upgraded a variety of devices constructed to improve venous access for long-term utilization. As with the introduction of any foreign object into the body, each of these devices has complications which may be life threatening and occasionally fatal. We present an unusual case of iatrogenic acute hydropericardium and cardiac tamponade caused by the percutaneous infusion of chemotherapeutic fluid via a right subclavian central venous implant system (Porta-Cath®). Failure to implant and monitor the device with a radiograph following placement according to manufacturer's guidelines and accepted standards of medical practice were causally related to an unusual complication, namely, perforation of the right cardiac ventricle by the catheter tip, resulting in sudden and unexpected cardiac death.
Twenty-Seven Years of Forensic Anthropology Casework in New Mexico
Authors: Komar D 
Published: August 2015

Abstract
A review of anthropological consult cases for the New Mexico Office of the Medical Investigator was conducted for the years 1974 through 2000. A total of 596 cases are summarized and information is presented on the sex and age of the individuals, season of recovery, depositional environment, body covering, time since death, perimortem trauma, postmortem animal activity, and skeletal element recovery. Results reveal a higher percentage of male victims (76%). No variation is seen in the seasonal distribution of cases. In cases with known time since death, 35% were recovered within one week while 30% had a postmortem interval exceeding one year. Depositional environments include surface (45%), burial (13%), and airplane crashes (9.5%). In 42% of the cases, no evidence of perimortem trauma was observed. Postmortem animal activity was noted in 46% of cases. Data presented in this study may prove useful in supporting expert witness testimony and generating future research models.
A Classifier for the SNP-Based Inference of Ancestry
Authors: Frudakis T , Venkateswarlu K , Thomas MJ , Gaskin Z , Ginjupalli S , Gunturi S , Ponnuswamy V , Natarajan S , Nachimuthu PK 
Published: August 2015

Abstract
Ancestral inference from DNA could serve as an important adjunct for both standard and future human identity testing procedures. However, current STR methods for the inference of ancestral affiliation have inherent statistical and technical limitations. In an effort to identify biallelic markers that can be used to infer ancestral affiliation from DNA, we screened 211 SNPs in the human pigmentation and xenobiotic metabolism genes. Allele frequencies of 56 SNPs (most from pigmentation genes) were dramatically different between groups of unrelated individuals of Asian, African, and European descent, and both observed and simulated log likelihood ratios revealed that the markers were of exceptional value for ancestral inference. Log likelihood ratios of the multilocus estimates of biological ancestry (EAE/EBA) ranged from 7 to 10, which are on par with the best of the STR batteries yet described. A linear classification method was developed for incorporating these SNPs into a classifier model that was 99, 98, and 100% accurate for identifying individuals of European, African, and Asian descent, respectively. The methods and markers we describe are therefore an important first step for the development of a practical multiplex test for the inference of ancestry in a forensics setting.
Glass-Containing Gunshot Residue Particles: A New Type of Highly Characteristic Particle?
Authors: Collins P , Coumbaros J , Horsley G , Lynch B , Kirkbride KP , Skinner W , Klass G 
Published: August 2015

Abstract
In 0.22 caliber rimfire ammunition, the primer often contains lead or lead and barium compounds. As residues from these primers do not contain lead, barium, and antimony, they cannot be uniquely classified as gunshot residue (GSR) under ASTM designation E 1588-95. In many types of 0.22 caliber rimfire ammunition, the cartridge contains a primer sensitized with glass. In this paper we describe a previously unreported type of GSR particle consisting of glass fused with other primer components. As there appear to be few potential environmental or occupational sources of particles composed of lead and barium compounds fused to glass, particularly borosilicate glass, these particles may have high evidential value. Scanning electron microscopy with energy dispersive X-ray detection (SEM-EDX) and time-of-flight secondary ion mass spectrometry (TOF-SIMS) were evaluated for the characterization of glass-containing GSR particles. The occurrence of glass-containing GSR particles was established in the residue from various brands of 0.22 caliber ammunition, and several sub-types were identified.
Volitional Impairment and the Sexually Violent Predator
Authors: Grinage BD 
Published: August 2015

Abstract
This article discusses psychiatry's limited conceptualization of volitional capacity and its application to sexually violent predator laws by exploring two legal opinions critical to predator case law (Kansas v. Hendricks and Kansas v. Crane). The author reviewed pertinent psychiatric literature on impaired volition to identify potential contributions and limitations that psychiatry may offer the legal field. Assessment of the ego dystonic nature of impaired self-regulation, utilization of recent advances in self-assessment and laboratory evaluation of impulsive behavior, and dimensional categorization of a volitional capacity construct are recommended as an approach to the assessment and understanding of an inability to control concept. This paper concludes that elements of volition may be psychiatrically evaluated in a way that contributes to the Court's understanding of that capacity. However, further study is needed to operationally define volitional capacity and address issues of assessment validity and reliability.
Osteoporosis Associated with the Treatment of Paraphilias: A Clinical Review of Seven Case Reports
Authors: Grasswick LJ , Bradford JMW 
Published: August 2015

Abstract
Osteoporosis and long-term androgen suppression in the treatment of paraphilias has been documented with surgical castration and the use of gonadotropin-releasing hormone agonists. The literature has suggested that the use of cyproterone acetate (CPA) may be protective against osteoporosis, although there are case reports of osteoporosis in men treated with CPA. This pilot study represents a case series of seven patients diagnosed with severe paraphilias and treated with CPA, leuprolide, or surgical castration. Two of the four patients treated with CPA developed significant osteoporosis, while the other two had normal bone density studies. The remaining three patients, one treated with leuprolide and two with surgical castration, had osteopenia. Based upon the current literature, the finding of significant osteoporosis in two of four patients treated with CPA, but not those treated with leuprolide or castration, is surprising. Monitoring of all patients treated with long-term androgen suppression for osteoporosis is suggested.
Inhibition of Bleach-Induced Luminol Chemiluminescence
Authors: Kent EJM , Elliot DA , Miskelly GM 
Published: August 2015

Abstract
The luminol chemiluminescence presumptive test for blood is based on the mild peroxidase activity of hemoglobin in basic peroxide solution. However, this test is subject to interference by strong oxidants, certain transition metal ions, and true peroxidases. This paper reports methods for reducing the interference caused by hypochlorite-containing bleaches. Amines such as 1,2-diaminoethane react rapidly with hypochlorite without interfering significantly with the hemoglobin-catalyzed oxidation. Thus, addition of 0.1 mol/L 1,2-diaminoethane to a standard luminol-peroxide spray lead to almost complete inhibition of hypochlorite-induced chemiluminescence while satisfactory chemiluminescence was still observed from bloodstains. If time allows, an alternative method for reducing interference from hypochlorite bleach is to wait several days until the bloodstains have dried thoroughly, by which time the hypochlorite will have decomposed.
The Fingernails of Mary Sullivan: Developing Reliable Methods for Selectively Isolating Endogenous and Exogenous DNA from Evidence
Authors: Cline RE , Laurent NM , Foran DR 
Published: August 2015

Abstract
The fingernails of Mary Sullivan, the last victim of the Boston Strangler, were examined to determine if any genetic information about the murderer could be obtained. The nails were extremely friable necessitating the development of new techniques for isolating and purifying DNA. DNA yields from nails were optimized by using a NaOH-based preparation technique, which was simple, efficient, and minimized handling. Methods for selectively and thoroughly removing exogenous material on nails were also developed through use of a species-specific PCR assay, wherein mitochondrial DNA from the nail could easily be differentiated from DNA of contaminating cells.
DNA Profiling of Azoospermic Semen Samples from Vasectomized Males by Using Y-PLEX™6 Amplification Kit
Authors: Shewale JG , Sikka SC , Schneida E , Sinha SD 
Published: August 2015

Abstract
Post-vasectomized azoospermic semen samples (N = 6) were analyzed for short tandem repeats (STR) on the Y-chromosome by using Y-PLEX™6 and the 310 Genetic Analyzer. We have observed a wide variation in the yield of extracted DNA from 12.5-1000 ng. This variation was attributed to the number of epithelial and/or white blood cells that are present in these azoospermic samples. DNA profiles of these vasectomized males were obtained for all six Y-STR loci, namely DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385 amplified by using the Y-PLEX™6.
Validation of Cytochrome b Sequence Analysis as a Method of Species Identification
Authors: Branicki W , Kupiec T , Pawlowski R 
Published: August 2015

Abstract
One of the stages of dealing with biological material submitted to forensic laboratories is species identification. The aim of the present work was to validate and assess the possibility of applying sequence analysis of the region coding cytochrome b as a method of species identification in the field of forensic science. DNA originating from individuals from major phyla of vertebrates was isolated by the organic method from various specimens. Extracted DNA was subjected to PCR and direct cycle sequencing using a universal pair of primers. The validation process, performed according to TWGDAM recommendations, revealed that the technique is a very sensitive and reliable method of species identification allowing analysis of tiny amounts of material and also degraded material, and can be useful in the field of forensic genetics. The case example presented here, concerning the determination of species origin of biological evidence collected from fatal road accident, confirms that analysis can be carried out even when there is no reference sample, and the sequences obtained can be assessed through analysis of their similarity to sequences for cytochrome b present in DNA databases.
Mechanisms of Unexpected Death in Tuberous Sclerosis
Authors: Byard RW , Blumbergs PC , James RA 
Published: August 2015

Abstract
Tuberous sclerosis complex is a protean autosomal dominant disorder characterized by multifocal tissue lesions arising from defects in cellular migration, proliferation, and differentiation. It has an association with sudden death. In the current study, review of all cases of sudden death due to tuberous sclerosis was undertaken at the Forensic Science Centre in Adelaide, Australia from 1991 to 2001, in addition to an analysis of cases from the literature. There were two local cases where unexpected death had occurred in individuals with known tuberous sclerosis, involving a 31-year-old male (epilepsy), and a 24-year-old female (massive hemorrhage into a renal angiomyolipoma). Fatal mechanisms in cases of tuberous sclerosis may be associated with underlying cardiovascular, renal and cerebral abnormalities. Sudden death may be due to cardiac arrhythmia, epilepsy, and intra-tumoral hemorrhage with additional complications including cardiac outflow obstruction, obstructive hydrocephalus, aneurysm rupture, and spontaneous pneumothorax. An awareness of the highly variable tissue manifestations of tuberous sclerosis and the mechanisms that may be responsible for death is necessary to establish correctly the diagnosis in occult cases (possibly with molecular confirmation), and to chart accurately organ changes in individuals with established disease.
Reexamination of a Measurement for Sexual Determination Using the Supero-Inferior Femoral Neck Diameter in a Modern European Population
Authors: Alunni-Perret V , Staccini P , Quatrehomme G 
Published: August 2015

Abstract
The present study reexamines the accuracy of the supero-inferior femoral neck diameter for the determination of sex using a modern sample of French individuals. In 1998, Seidemann et al. used this univariate method for sex determination with the Hamann-Todd collection. Stojanowski and Seidemann in 1999 tested previous results on a modern sample taken from the University of New Mexico and concluded that the Caucasian male samples exhibited no significant differences between individuals born before and after 1900, but the Caucasian female subgroup did exhibit differences with an increase of the SID in the modern sample. The current study compares the previous results of the supero-inferior femoral neck diameter with a modern sample of elderly French individuals born after 1910. Both sides of the femur were measured. No statistical difference was found between the right and left side ( p = 0.31). The results showed a significant difference between the pre-1900 and the modern sample, with an increase in femoral neck diameter in modern populations. The comparison of the SID values between the two modern samples (Mexico and Nice) showed no significant differences in the femoral neck diameter in the two male subgroups ( p = 0.05), but the measurements of the SID in the female subgroup did exhibit significant differences with an increase of the neck femoral diameter ( p &lt; 0.01) in the modern French population. These results demonstrate an increase in the neck femoral morphology in the elderly European French females samples.
Characterization of Pyrotechnic Reaction Residue Particles by SEM/EDS
Authors: Kosanke KL , Dujay RC , Kosanke B 
Published: August 2015

Abstract
Today the method commonly used for detecting gunshot residue is through the combined use of scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). In recent years, this same methodology began to find use in detecting and characterizing pyrotechnic reaction residue (PRR) particles whether produced by explosion or burning. This is accomplished by collecting particulate samples from a surface in the immediate area of the pyrotechnic reaction. Suspect PRR particles are identified by their morphology (typically 1 to 20 µm spheroidal particles) using an SEM; then they are analyzed for the elements they contain using X-ray EDS. This can help to identify the general type of pyrotechnic composition involved.
Further Studies on Spot Tests and Microcrystal Tests for Identification of Cocaine
Authors: Swiatko J , De Forest PR , Zedeck MS 
Published: August 2015

Abstract
The presence of cocaine in illicit drug samples is still being determined in some laboratories using spot tests and microcrystal tests. Seventeen chemical species were tested using three different spot tests (Wagner, Marquis, and cobalt thiocyanate followed by stannous chloride reactions) and two microcrystal tests (gold chloride and platinic chloride) to determine whether the results could be differentiated from the results of these tests on cocaine. The data obtained indicated that nine of the 17 compounds gave results similar to those from cocaine using the three spot tests, but that the results from microcrystal testing allowed for differentiation of all nine compounds from cocaine.
Lessons from Srebrenica: The Contributions and Limitations of Physical Anthropology in Identifying Victims of War Crimes
Authors: Komar D 
Published: August 2015

Abstract
In July 1995, the town of Srebrenica fell to Bosnian-Serb forces, leaving more than 7000 Muslim men missing and presumed dead. Anthropologists participating in the identification process were faced with a unique problem: the victims appeared identical. All were adult males of a single ethnic group. Decomposition as well as the absence of antemortem (AM) medical and dental records confounded identification. As of December 1999, only 63 men had been positively identified using DNA, personal effects, and identification papers. Are current anthropological methods of sex, age, and stature estimation and AM trauma assessment sufficiently accurate to differentiate the remaining victims and aid in their identification? Comparisons of relative-reported AM information and postmortem examination records for 59 of the 63 identified individuals indicated that while all individuals were sexed correctly, only 42.4% were accurately aged and 29.4% had a stature estimate that included their reported height.
Discriminant Function Sexing of Fragmentary and Complete Femora: Standards for Contemporary Croatia
Authors: Šlaus M , Strinović D , Škavić J , Petrovečki V 
Published: August 2015

Abstract
Determining sex is one of the first and most important steps in identifying decomposed corpses or skeletal remains. Previous studies have demonstrated that populations differ from each other in size and proportion and that these differences can affect metric assessment of sex. This paper establishes standards for determining sex from fragmentary and complete femurs in a modern Croatian population. The sample is composed of 195 femora (104 male and 91 female) from positively identified victims of the 1991 War in Croatia. Six discriminant functions were generated, one using seven variables, three using two variables, and two employing one variable. Results show that complete femora can be sexed with 94.4% accuracy. The same overall accuracy, with slight differences in male/female accuracy, was achieved using a combination of two variables defining the epiphyses, and with the variable maximum diameter of the femoral head.