bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of enhanced bolus flavors on oropharyngeal swallow in patients treated for head and neck cancer
Authors: Roa Pauloski Barbara , Logemann Jerilyn A. , Rademaker Alfred W. , Lundy Donna , Sullivan Paula A. , Newman Lisa A. , Lazarus Cathy , Bacon Mary 
Published: August 2012

AbstractBackgroundTreatment for head and neck cancer can reduce peripheral sensory input and impair oropharyngeal swallow. This study examined the effect of enhanced bolus flavor on liquid swallows in these patients.MethodsFifty‐one patients treated for head and neck cancer with chemoradiation or surgery and 64 healthy adult control subjects served as subjects. All were randomized to receive sour, sweet, or salty bolus flavor. Patients were evaluated at 7–10 days, 1 month, and 3 months after completion of tumor treatment. Control subjects received 1 assessment.ResultsAll bolus flavors affected oropharyngeal swallow; sour flavor significantly shortened pharyngeal transit time across all evaluations.ConclusionsSour flavor influenced the swallow of patients treated for head and neck cancer, as well as that of control subjects in a manner similar to those with neurologic impairment observed in an earlier study. Sour flavor may improve the speed of pharyngeal transit regardless of whether a patient has suffered peripheral or central sensory damage. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Rehabilitation of a parotidectomy patient—A systematic approach
Authors: Militsakh Oleg N. , Sanderson Jeremy A. , Lin Derrick , Wax Mark K. 
Published: August 2012

AbstractParotidectomy is a common surgical procedure. Resultant contour defect, Frey's syndrome, and facial nerve rehabilitation deserve special consideration. Microsurgical techniques provide unparallel advantage for reconstruction of large‐volume defects. Same‐stage reconstruction of the defect is advocated and often beneficial to the patient. The importance of full communication between the extirpative and reconstructive surgeon cannot be underscored. Often, institutional and personal biases must be overcome to provide best quality care for the patient. This article provides a comprehensive review of the medical literature on the subject and contrives a systematic approach to the use of various reconstructive techniques. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Abnormal anatomy of the superficial branch of the radial nerve
Authors: Lindau Robert H. , Wax Mark K. 
Published: August 2012

AbstractBackgroundThe superficial branch of the radial nerve is frequently encountered when performing the harvest for a radial forearm free tissue transfer.Methods and ResultsWe present an abnormal course of this nerve which has not been described in the literature. During a harvest of a radial forearm free flap, we encountered a nerve that was not deep to the brachioradialis muscle in the proximal arm; rather, the superficial branch of the radial nerve was found to be superficial to this muscle through its entire course. An in‐depth literature review was performed, and there was no documented abnormality identified resembling the abnormality we encountered.ConclusionKnowledge of the normal and abnormal anatomy is essential for the microvascular surgeon to assure the safety of this nerve. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Low‐grade and high‐grade mammary carcinomas in WAP‐T transgenic mice are independent entities distinguished by Met expression
Authors: Otto Benjamin , Gruner Katharina , Heinlein Christina , Wegwitz Florian , Nollau Peter , Ylstra Bauke , Pantel Klaus , Schumacher Udo , Baumbusch Lars O. , Martin‐Subero José Ignacio , Siebert Reiner , Wagener Christoph , Streichert Thomas , Deppert Wolfgang , Tolstonog Genrich V. 
Published: August 2012

AbstractMammary carcinomas developing in SV40 transgenic WAP‐T mice arise in two distinct histological phenotypes: as differentiated low‐grade and undifferentiated high‐grade tumors. We integrated different types of information such as histological grading, analysis of aCGH‐based gene copy number and gene expression profiling to provide a comprehensive molecular description of mammary tumors in WAP‐T mice. Applying a novel procedure for the correlation of gene copy number with gene expression on a global scale, we observed in tumor samples a global coherence between genotype and transcription. This coherence can be interpreted as a matched transcriptional regulation inherited from the cells of tumor origin and determined by the activity of cancer driver genes. Despite common recurrent genomic aberrations, e.g. gain of chr. 15 in most WAP‐T tumors, loss of chr. 19 frequently occurs only in low‐grade tumors. These tumors show features of “basal‐like” epithelial differentiation, particularly expression of keratin 14. The high‐grade tumors are clearly separated from the low‐grade tumors by strong expression of the Met gene and by coexpression of epithelial (e.g. keratin 18) and mesenchymal (e.g. vimentin) markers. In high‐grade tumors, the expression of the nonmutated Met protein is associated with Met‐locus amplification and Met activity. The role of Met as a cancer driver gene is supported by the contribution of active Met signaling to motility and growth of mammary tumor‐derived cells. Finally, we discuss the independent origin of low‐ and high‐grade tumors from distinct cells of tumor origin, possibly luminal progenitors, distinguished by Met gene expression and Met signaling.
NY‐ESO‐1 antigen‐reactive T cell receptors exhibit diverse therapeutic capability
Authors: Sommermeyer Daniel , Conrad Heinke , Krönig Holger , Gelfort Haike , Bernhard Helga , Uckert Wolfgang 
Published: August 2012

AbstractThe cancer‐testis antigen NY‐ESO‐1 has been used as a target for different immunotherapies like vaccinations and adoptive transfer of antigen‐specific cytotoxic T cells, as it is expressed in various tumor types and has limited expression in normal cells. The in vitro generation of T cells with defined antigen specificity by T cell receptor (TCR) gene transfer is an established method to create cells for immunotherapy. However, an extensive characterization of TCR which are candidates for treatment of patients is crucial for successful therapies. The TCR has to be efficiently expressed, their affinity to the desired antigen should be high enough to recognize low amounts of endogenously processed peptides on tumor cells, and the TCR should not be cross‐reactive to other antigens. We characterized three NY‐ESO‐1 antigen‐reactive cytotoxic T lymphocyte clones which were generated by different approaches of T cell priming (autologous, allogeneic), and transferred their TCR into donor T cells for more extensive evaluations. Although one TCR most efficiently bound MHC‐multimers loaded with NY‐ESO‐1 peptide, T cells expressing this transgenic TCR were not able to recognize endogenously processed antigen. A second TCR recognized HLA‐A2 independent of the bound peptide beside its much stronger recognition of NY‐ESO‐1 bound to HLA‐A2. A third TCR displayed an intermediate but peptide‐specific performance in all functional assays and, therefore, is the most promising candidate TCR for further clinical development. Our data indicate that multiple parameters of TCR gene‐modified T cells have to be evaluated to identify an optimal TCR candidate for adoptive therapy.
Prevalence of high‐risk human papillomavirus and cervical intraepithelial neoplasias in a previously unscreened population—A pooled analysis from three studies
Authors: Basu Partha , Mittal Srabani , Bhaumik Suchismita , Mandal Shyam Sunder , Samaddar Anusree , Ray Chinmayi , Siddiqi Maqsood , Biswas Jaydip , Sankaranarayanan Rengaswamy 
Published: August 2012

AbstractPopulation prevalence of human papillomavirus (HPV) and cervical intraepithelial neoplasias (CIN) is an important indicator to judge the disease burden in the community, to monitor the performance of cervical cancer screening program and to assess the impact of HPV vaccination program. India being a country without any cervical cancer screening program has no published data on the population prevalence of CIN and only a few large community‐based studies to report the high‐risk HPV prevalence. The objective of our study was to study HPV and CIN prevalence in a previously unscreened population. We pooled together the results of three research studies originally designed to assess the performance of visual inspection after acetic acid application and Hybrid Capture 2 (HC 2). Nearly 60% of the screened women had colposcopy irrespective of their screening test results. The diagnosis and grading of cervical neoplasias were based on histology. The age standardized prevalence of HPV by HC 2 test was 6.0%. Age‐adjusted prevalence of CIN1 and CIN2 was 2.3% and 0.5%, respectively. The age‐adjusted prevalence of CIN3 was 0.4% and that of invasive cancer was 0.2%. The prevalence of high‐risk HPV was relatively low in the population we studied, which is reflected in the low prevalence of high‐grade CIN. The prevalence of CIN3 remained constant across age groups due to absence of screening.
Automatic quantification of subcutaneous and visceral adipose tissue from whole‐body magnetic resonance images suitable for large cohort studies
Authors: Wald Diana , Teucher Birgit , Dinkel Julien , Kaaks Rudolf , Delorme Stefan , Boeing Heiner , Seidensaal Katharina , Meinzer Hans‐Peter , Heimann Tobias 
Published: August 2012

AbstractPurpose:To develop an automated method with which to distinguish metabolically different adipose tissues in a large number of subjects using whole‐body magnetic resonance imaging (MRI) datasets for improving the understanding of chronic disease risk predictions associated with distinct adipose tissue compartments.Materials and Methods:In all, 314 participants were scanned using a 1.5T MRI‐scanner with a 2‐point Dixon whole‐body sequence. Image segmentation was automated using standard image processing techniques and knowledge‐based methods. Abdominal adipose tissue was separated into subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) by statistical shape models. Bone marrow was removed to provide a more accurate measurement of adipose tissue. To assess segmentation accuracy, ground‐truth segmentations in 52 images were performed manually by one operator. Due to the high effort of manual delineation, manual segmentation was limited to seven slices per volume.Results:Volumetric differences were 3.30 ± 2.97% and 6.22 ± 5.28% for SAT and VAT, respectively. The systematic error shows an overestimation of 4.22 ± 7.01% for VAT and 0.37 ± 4.45% for SAT. Coefficients‐of‐variation from repeated measurements were: 3.50 ± 2.93% for VAT and 0.35 ± 0.26% for SAT. The approach of removing bone marrow worked well in most body regions. Only occasionally the method failed for knees and/or shinbone, which resulted in an overestimation of SAT by 3.14 ± 1.45%.Conclusion:We developed a fully automatic process to assess SAT and VAT in whole‐body MRI data. The method can support epidemiological studies investigating the relationship between excess body fat and chronic diseases. J. Magn. Reson. Imaging 2012; 36:1421–1434. © 2012 Wiley Periodicals, Inc.
Breast imaging: is the sonographic descriptor of orientation valid for magnetic resonance imaging?
Authors: Oliveira Tatiane M.G. , Brasileiro Sant'Anna Tatiana Kelly , Mauad Fernando M. , Elias Jorge , Muglia Valdair F. 
Published: August 2012

AbstractPurpose:To evaluate if the Breast Imaging Reporting and Data System (BI‐RADS) ultrasound descriptor of orientation can be used in magnetic resonance imaging (MRI).Materials and Methods:We conducted a retrospective study to evaluate breast mass lesions identified by MRI from 2008 to 2010 who had ultrasound (US) and histopathologic confirmation. Lesions were measured in the craniocaudal (CC), anteroposterior (AP), and transverse (T) axes and classified as having a nonparallel orientation, longest axis perpendicular to Cooper's ligaments, or in a parallel orientation when the longest axis is parallel to Cooper's ligaments. The MR image data were correlated with the US orientation according to BI‐RADS and histopathological diagnosis.Results:We evaluated 71 lesions in 64 patients. On MRI, 27 lesions (38.0%) were nonparallel (8 benign and 19 malignant), and 44 lesions (62.0%) were parallel (33 benign and 11 malignant). There was significant agreement between the lesion orientation on US and MRI (kappa value = 0.901). The positive predictive values (PPV) for parallel orientation malignancy on MR and US imaging were 70.4% and 73.1%, respectively.Conclusion:A descriptor of orientation for breast lesions can be used on MRI with PPV for malignant lesions similar to US. J. Magn. Reson. Imaging 2012; 36:1383–1388. © 2012 Wiley Periodicals, Inc.
α2β1 integrin and RhoA mediates electric field‐induced ligament fibroblast migration directionality
Authors: Tsai Cheng‐Hsien , Lin Bo‐Jiang , Chao Pen‐Hsiu Grace 
Published: August 2012

AbstractGuided cell migration is important in tissue development, repair, and engineering. We have previously demonstrated that applied electric fields (EFs) enhanced and directed ligament fibroblast migration and collagen production, depending on EF parameters. Electrical stimulation is widely used for the treatment of pain and to promote wound healing. In orthopaedic practices, applied EFs promote bone healing and ligament repair in vivo. In the current study, stimulation waveforms used in physical therapy for promoting tissue repair were adapted to examine their effects on ACL fibroblast migration. Using different waveform and field strengths, we discovered a decoupling of cell motility and directionality, which suggests disparate mechanisms. Integrin, a major extracellular matrix receptor, polarized in response to applied EFs and controlled cell directionality and signaling. Furthermore, we demonstrated that RhoA is a mediator between integrin aggregation and directed cell migration. Polarization is essential in directed cell migration and our study establishes an outside‐in signaling mechanism for EF‐induced cell directionality. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 322–327, 2013
Effect of growing location, malaxation duration and citric acid treatment on the quality of olive oil
Authors: Chih HuiJun , James Anthony P , Jayasena Vijay , Dhaliwal Satvinder S 
Published: August 2012

AbstractBACKGROUND: The total phenolic compounds of olive oil exert antiradical activity at cellular level and can prevent cardiovascular disease, metabolic syndrome and cancer. Increased awareness of its health benefits has increased the consumption of olive oil around the world. An alternative processing technique effective in increasing the amount of oil extracted while maintaining the oil quality is needed to meet the rising global demand for olive oil.RESULTS: Addition of 0.3 g mL−1 citric acid at 1:1000 (v/w) to olive paste followed by a 30 min malaxation period significantly increased the oil recovery, concentration of total phenolic compounds and antiradical activity by 46.23, 120.27 and 31.48% respectively. While there was no significant effect on the acidity, the peroxide value was significantly reduced by 63.85%. The organoleptic characteristics of the olive oil extracted with citric acid were also comparable to those of the control.CONCLUSION: Addition of 0.3 g mL−1 citric acid (i.e. 30% w/v) at 1:1000 (v/w) to olive paste followed by a 30 min malaxation period in a Blixer® 4.0 blender is the most promising extraction technique to improve the oil recovery, concentration of total phenolic compounds and antiradical activity of the extracted olive oil without compromising other quality parameters. © 2012 Society of Chemical Industry
Angiotensin‐converting enzyme (ACE)‐inhibitory activity of boza, a traditional fermented beverage
Authors: Kancabaş Aslı , Karakaya Sibel 
Published: August 2012

AbstractBackgroundIn this study the angiotensin‐converting enzyme (ACE)‐inhibitory activity of boza and protein fractions of boza separated according to molecular weight was determined. In addition, the effect of in vitro digestion on ACE‐inhibitory activity was investigated.ResultsThe protein content, ACE‐inhibitory activity and IC50 value of boza were 1.0896 ± 0.08%, 76.76 ± 14.93% and 7.2 ± 0.28 µg protein mL−1 respectively. The protein hydrolysate was separated into three fractions according to molecular weight (MW), i.e. MW < 5000 Da, 5000 < MW < 10 000 Da and 10 000 < MW < 20 000 Da. The lowest IC50 value (0.268 ± 0.07 µg protein mL−1) was found for the fraction with 5000 < MW < 10 000 Da (P < 0.05). After in vitro digestion the ACE‐inhibitory activities of stomach and intestine dialysates were almost the same (P > 0.05). The IC50 value of stomach digest was determined as 2.06 ± 0.32 (µg protein mL−1). However, the IC50 value of intestine digest could not be determined, because all dialysates with different protein concentrations displayed ACE‐inhibitory activity greater than 50%.ConclusionThe findings indicate that boza, protein hydrolysate, fractionated hydrolysates and dialysates obtained after in vitro digestion contain bioactive compounds with different ACE‐inhibitory activities. Based on these results, boza can be considered as a good source of ACE‐inhibitory peptides.
Spatial frequency domain imaging of port wine stain biochemical composition in response to laser therapy: A pilot study
Authors: Mazhar Amaan , Sharif Seyed A. , Cuccia J. David , Nelson J. Stuart , Kelly Kristen M. , Durkin Anthony J. 
Published: August 2012

AbstractBackground and ObjectiveObjective methods to assess port wine stain (PWS) response to laser treatment have been the subject of various research efforts for several years. Herein, we present a pilot study using a newly developed, light emitting diode (LED) based spatial frequency domain imaging (SFDI) device to record quantitatively biochemical compositional changes in PWS after laser therapy.Study Design/Patients and MethodsA SFDI system was used to image before, and after, five PWS treatment sessions [n = 4 subjects (one subject was imaged before and after two consecutive laser treatments)]. SFDI derived wide‐field optical properties (absorption and scattering) and tissue chromophore concentrations including oxy‐hemoglobin (ctO2Hb), deoxy‐hemoglobin (ctHHb), total hemoglobin (ctTHb), and tissue oxygen saturation (stO2) are presented for skin imaged prior to and immediately after laser treatment. The SFDI derived images were analyzed by comparing the above measurements in PWS to those of normal skin and tracking changes immediately after laser exposure.ResultsElevated oxy‐hemoglobin (>20%) and tissue oxygen saturation (>5%) were measured in all PWS lesions and compared to values for normal skin prior to treatment. Laser treatment resulted in an increase in deoxy‐hemoglobin (>100%), decrease in tissue oxygen saturation (>10%), and reduced scattering (>15%) in all PWS lesions. One subject was followed before and after two consecutive laser treatments and the overall improvement in PWS lesion blanching was quantitatively assessed by measuring a 45% decrease in dermal blood volume.ConclusionSFDI is a rapid non‐contact wide‐field optical technique that shows potential as an imaging device that can be used to quantify biochemical compositional changes in PWS after laser therapy. Future work will investigate the potential of SFDI to provide intra‐operative guidance for laser therapy of PWS lesions on an individual patient basis. Lasers Surg. Med. 44: 611–621, 2012. © 2012 Wiley Periodicals, Inc.
The topography of DNA methylation in the non‐neoplastic colonic mucosa surrounding colorectal cancers
Authors: Subramaniam Manish Mani , Loh Marie , Chan Jason Yongsheng , Liem Natalia , Lim Pei Li , Peng Yong Wei , Lim Xn Yii , Yeoh Khay Guan , Iacopetta Barry , Soong Richie , Salto‐Tellez Manuel 
Published: August 2012

AbstractThe degree of gene hypermethylation in non‐neoplastic colonic mucosa (NNCM) is a potentially important event in the development of colorectal cancer (CRC), particularly for the subgroup with a CpG island methylator phenotype (CIMP). In this study, we aimed to use an unbiased and high‐throughput approach to evaluate the topography of DNA methylation in the non‐neoplastic colonic mucosa (NNCM) surrounding colorectal cancer (CRC). A total of 61 tissue samples comprising 53 NNCM and 8 tumor samples were obtained from hemicolectomy specimens of two CRC patients (Cases 1 and 2). NNCM was stripped from the underlying colonic wall and samples taken at varying distances from the tumor. The level of DNA methylation in NNCM and tumor tissues was assessed at 1,505 CpG sites in 807 cancer‐related genes using Illumina GoldenGate® methylation arrays. Case 1 tumor showed significantly higher levels of methylation compared to surrounding NNCM samples (P < 0.001). The average level of methylation in NNCM decreased with increasing distance from the tumor (r = −0.418; P = 0.017), however this was not continuous and “patches” with higher levels of methylation were observed. Case 2 tumor was less methylated than Case 1 tumor (average β‐value 0.181 vs. 0.415) and no significant difference in the level of methylation was observed in comparison to the surrounding NNCM. No evidence was found for a diminishing gradient of methylation in the NNCM surrounding CRC with a high level of methylation. Further work is required to determine whether CIMP+ CRC develop from within “patches” of NCCM that display high levels of methylation. © 2012 Wiley Periodicals, Inc.
Identification of a liver cirrhosis signature in plasma for predicting hepatocellular carcinoma risk in a population‐based cohort of hepatitis B carriers
Authors: Liu Chia‐Chi , Wang Ya‐Hui , Chuang Eric Y , Tsai Mong‐Hsun , Chuang Ya‐Hui , Lin Chih‐Lin , Liu Chun‐Jen , Hsiao Bo‐Yu , Lin Shi‐Ming , Liu Li‐Yu , Yu Ming‐Whei 
Published: August 2012

AbstractLiver cirrhosis is a critical state in the natural course of hepatocellular carcinoma (HCC). We sought to investigate the potential of in‐depth proteomics to reveal plasma protein signatures that reflect common networks/pathways of liver cirrhosis, and to determine whether the cirrhosis‐related signature in plasma is linked to the development of HCC among hepatitis B virus (HBV) carriers. We first compared plasma protein profiles using a 174‐antibody microarray system between three groups of HBV carriers with different Child's grades of cirrhosis, which revealed a panel of 45 differentially expressed proteins with a high accuracy for discriminating Child's B/C. Ingenuity Pathway Analysis identified two main up‐regulated networks connecting the 45 proteins that were most enriched for genes in the pathway of hepatic stellate cell activation. A parsimonious subset of 11 pathway‐based proteins was then selected for quantification to correlate with HCC risk among 49 HCC cases and 50 controls in a nested case–control study within a 16‐yr follow‐up cohort of HBV carriers. A high risk score derived from a principal component analysis, which was used to extract the cluster structure of the 11 proteins, was associated with HCC (odds ratio = 4.83, 95% confidence interval: 1.26–18.56) even after adjustment for viral and clinical variables, implying the involvement of a pattern of coordinated proteins. Stepwise logistic regression on the 11 proteins revealed ICAM‐2 as an independent predictor for HCC. These findings may give further insight into the pathobiology of hepatocarcinogenesis, allow testing of the cirrhosis‐related plasma protein signature as a potential predictive biomarker for HCC. Copyright © 2012 Wiley Periodicals, Inc. © 2013 Wiley Periodicals, Inc.