bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spindle Cell Hemangioendothelioma: Report of Three Cases
Authors: Terashi Hiroto , Itami Satoshi , Kurata Sotaro , Sonoda Tadashige , Takayasu Susumu , Yokoyama Shigeo 
Published: April 2015

AbstractThree cases of spindle cell hemangioendothelioma were studied. Two had multiple nodules and the third had a single, recurrent lesion in the dermis or subcutis of the distal extremities. The tumor initiated at the age of 3 months in one patient and at the ages of 11 or 12 years in the remaining two. Local recurrence after surgical excision was observed in all cases. Microscopically the tumors were composed of two components; cavernous blood spaces and a proliferation of spindle cells. Among these spindle cells, there were occasional epithelioid endothelial cells with intracytoplasmic lumina. Phleboliths were observed in two cases. Most of the endothelial cells lining the cavernous spaces were positive for factor VIII‐associated antigen in all cases; the intracytoplasmic lumina were also positive in two cases. The spindle cells were negative for that antigen in all cases. However, they were positive for desmin in one case. Electronmicroscopically, the spindle cells resembled primitive mesenchymal cells.
Increased Production of Transforming Growth Factor‐α in Psoriatic Epidermis
Authors: Higashiyama Mari , Matsumoto Kunio , Hashimoto Koji , Yoshikawa Kunihiko 
Published: April 2015

AbstractTo elucidate the involvement of transforming growth factor‐α (TGF‐α) in the pathogenesis of psoriasis, we measured TGF‐α levels in the extracts from six normal epidermis and four psoriatic involved epidermis samples by enzyme‐linked sorbent assay using monoclonal antibody specific for TGF‐α. The amount of TGF‐α in the extracts of normal epidermis was 1.45 ± 1.06 ng/g of wet tissue, while the amount in psoriatic involved epidermis was 6.71 ± 0.75 ng/g of wet tissue. The TGF‐α level in psoriatic involved epidermis was thus 4.62 fold higher than that of normal epidermis (P<0.001). TGF‐α binds to epidermal growth factor receptors and functions as an autocrine growth factor for epidermal keratinocytes. Therefore, the increased levels of TGF‐α may be involved in the induction or the maintenance of hyperproliferation of psoriatic epidermal keratinocytes.
A Case of Pemphigus Vulgaris in a Six‐year‐old Girl
Authors: Shoda Yukiko , Hashimoto Koji , Matsuoka Yukari , Yoshikawa Kunihiko 
Published: April 2015

AbstractA case of pemphigus vulgaris in a six‐year‐old Japanese girl is presented. She first developed vesicles and ulcerations in oral and laryngeal mucous membranes, showing a hoarse voice and fits of coughing with excessive slavering. She had skin blisters six months later. Biopsy of the skin lesion demonstrated the intraepidermal blister in a suprabasal location. Direct immunofluorescence (IF) of the skin lesion revealed deposits of IgG and C3. Indirect IF showed serum anti‐ICS antibody titer at 1:640. She was diagnosed as having pemphigus vulgaris. Treatment with oral prednisolone (1 mg/kg) proved effective. This is the first case of infantile pemphigus vulgaris in Japan.
Discordant Expression of Tuberous Sclerosis in Monozygotic Twins
Authors: Kondo Shigeo , Yamashina Ushio , Sato Noriaki , Aso Kazuo 
Published: April 2015

AbstractA set of 20‐year‐old female Japanese twins, most probably monozygotic, had clinical evidence of tuberous sclerosis (TS). They were discordant for symptoms. One of the twins exhibited facial red‐brown papules (adenoma sebaceum), a dorsal shagreen patch, intracerebral calcifications, angiomyolipoma in the right kidney, and hypopigmented macules; the other had only a few hypopigmented macules. Modification of TS gene expression by the effects of environmental (extrinsic) factors is suggested.
Expression of OKM5 Antigen on Keratinocytes in Some Dermatoses
Authors: He Chundi , Zhao Yuming , Chen Hong‐Duo 
Published: April 2015

AbstractEmploying an avidin‐biotin complex immunoperoxidase technique, it was found that there was OKM5 but no OKM1 antigen expression on the keratinocytes of the upper epidermis from all the studied specimens of seborrheic keratosis, verrucous nevus, Bowen's disease, BCC, SCC, MM, lichen planus, psoriasis vulgaris, condyloma acuminatum, and sporotrichosis as well as in two of eight cases of nevus pigmentosus. Our findings indicate that OKM5 antigen expression on epidermal keratinocytes is usually associated with dermal infiltration. It is assumed that human keratinocytes in cutaneous lesions, like the OKM1− OKM5+ monocyte/macrophage lineages, might play certain roles in immune responses and possibly function as antigen‐presenting cells.
A Case of Seminal Fluid Allergy Successfully Treated with Immunotherapy in a One‐day Rush Procedure
Authors: Boom Bart W. , van Toorenenbergen Albert W. , Nierop Geesje , van der Schroeff Jan‐Gerrit 
Published: April 2015

AbstractA 28‐year‐old woman was treated for seminal fluid allergy with immunotherapy using a one‐day ‘rush’ procedure. Apart from minor anaphylactic symptoms, no serious side effects were noted during or after hyposensitization or boostering. Five weeks after the start of the hyposensitization, she was free of symptoms after unprotected coitus.
Purpura Fulminans Secondary to Xanthomonas Maltophilia Sepsis in an Adult with Aplastic Anemia
Authors: Kato Naoko , Morioka Tokiyo 
Published: April 2015

AbstractPurpura fulminans is a rare disease characterized by purpura ecchymosis, hypotension, and fever associated with disseminated intravascular coagulation. It often begins as a benign infectious process and subsequently progresses to a severe, catastrophic outcome. It is recognized to originate from congenital or acquired protein C deficiency. We present an unusual case of an adult with Xanthomonas maltophilia sepsis that subsequently developed into purpura fulminans with involvement of the four extremities. We discuss the importance of the protein C system in coagulation homeostasis and its relationship to purpura fulminans.
Epidermolysis Bullosa Acquisita in Identical Twins
Authors: Murakami Shinji , Shiraishi Satoshi , Miki Yoshiharu 
Published: April 2015

AbstractIdentical twin sisters developed generalized erythema and bullae on skin and mucous membranes at 18 and 19 years of age. Atrophic scars and milia were formed later. Indirect immunofluorescence (IF) study of the separated skin by incubation in 1.0M NaCl showed anti‐basement membrane zone (BMZ) antibodies bound to the dermal side. The HLA‐DR typing demonstrated DR2/DW11. Pulse therapy resulted in marked clinical improvement. This is the first report of epidermolysis bullosa acquisita (EBA) in identical twins and suggests the possibility that the disease may have a genetic component.
A Case of Cutaneous Squamous Cell Carcinoma Associated with Sporadic Porphyria Cutanea Tarda Due to Liver Disorder after Schistosoma japonicum Infection
Authors: Ohtake Naoto , Takayama Osami , Uno Akihiko , Kubota Yasuo , Shimada Shinji , Tamaki Kunihiko 
Published: April 2015

AbstractA 56‐year‐old man suffered from cutaneous squamous cell carcinoma (cutaneous SCC) which occurred on a cutaneous lesion of sporadic porphyria cutanea tarda (sporadic PCT).His liver function decreased from the time he was infected with Schistosoma japonicum at the age of 10. He drank a little alcohol.Erythematous maculae with blisters or erosions occurred on sun‐exposed areas of his skin when he was 52. His urine continued to be red. After detailed examinations including liver biopsy, he was diagnosed as having sporadic PCT due to liver disorder after infection with Schistosoma japonicum.At the age of 56, a small red papule arose on his right earlobe at the site of a sporadic PCT lesion. The papule rapidly enlarged with ulceration; this completely destroyed his right earlobe, which was covered with odoriferous yellow‐white necrotic tissue. The tumor then extended to his right preauricular area with ulceration. A skin biopsy confirmed well‐differentiated cutaneous SCC.The association of cutaneous SCC with sporadic PCT has not been previously reported; we think that the association is significant Such an occurrence may have been induced by either direct or indirect effects of ultraviolet light or a scar formed by the sporadic PCT.
Drug Eruptions Following Treatment with Prodrugs: A Review of the Reported Cases in Japan from 1984 to 1989
Authors: Imayama Shuhei , Fukuda Hidezo , Hori Yoshiaki 
Published: April 2015

AbstractA review of 872 cases of drug eruption reported in the Japanese dermatological journals from 1984 to 1989 has revealed a growing subset related to treatment with prodrugs, in which the responsible agent may be the metabolite rather than the prodrug itself. This situation is the result of the presence in tissues and fluids of only the metabolite following absorption and degradation of the prodrug. Because of this condition, patients with drug eruptions after prodrug treatment may develop positive patch test and/or lymphocyte stimulation test reactions not only with the introduced prodrug but also with the metabolite produced in vivo and further with other prodrugs which degrade into the same metabolite.
Transcutaneous PO2 and PCO2 Measurements in Various Skin Lesions
Authors: Takiwaki Hirotsugu , Arase Seiji , Nakanishi Hideki , Takeda Katsuyuki 
Published: April 2015

AbstractLow tcPO2 levels were observed in various skin lesions, indicating that most pathological changes in the skin induce such a reduction. In contrast, the tcPCO2 changed less, with the exception of marked elevations in bullous or prenecrotic lesions. This procedure may have practical applications in clinical dermatology, particulary in quantitating the response to therapy and in predicting a necrotic change.
Immunohistochemistry of Cutaneous Graft‐versus‐Host Disease after Allogeneic Bone Marrow Transplantation
Authors: Girolomoni Giampiero , Pincelli Carlo , Zambruno Giovanna , Andreani Marco , Giardini Claudio , Lucarelli Guido , Giannetti Alberto 
Published: April 2015

AbstractGraft‐versus‐host disease (GVHD) is an immunologically mediated disease occurring most frequently after allogeneic bone marrow transplantation. The aim of this study was to evaluate the contribution of immunohistochemistry in the diagnosis of cutaneous GVHD. Patients transplanted for either leukemia or β‐thalassemia were included in the study. Skin lesions of acute and chronic GVHD were examined both by direct immunofluorescence to detect immunoglobulin deposits and by an avidin‐biotin‐peroxidase complex technique to evaluate the inflammatory cell infiltrate. Epidermal and dermal fluorescent bodies (IgG and IgM) were frequently found in both acute and chronic GVHD. Most of the infiltrating cells were CD3+ T lymphocytes, with CD8+ cells representing the major cell population invading the epidermis both in acute GVHD and in chronic lichenoid GVHD. A small proportion of the dermal cells were CD14+ macrophages; no B cells were detected. HLA‐DR, but not HLA‐DQ antigens, were variably expressed by keratinocytes in all cases of acute GVHD and in chronic lichenoid GVHD. KL‐1, a monoclonal antikeratin antibody specific for the 56.5 KD acidic polypeptide usually present in suprabasal keratinocytes, stained all epidermal layers, including the basal layer. Langerhans cells were dramatically reduced in number in the epidermis of both acute and chronic lichenoid GVHD. It is concluded that immunohistologic analysis may be supportive in the diagnosis of acute and early chronic lichenoid cutaneous GVHD.
Trichilemmal Tumor Arising in a Seborrheic Keratosis: Analysis of Cell Kinetics by BrdU Staining
Authors: Kitagawa Kayoko , Amagai Masayuki , Miyakawa Syun‐ichi , Hayakawa Kazuhito , Ueda Masakazu , Shimizu Nobuyoshi 
Published: April 2015

AbstractWe report a case of a 74‐year‐old male with a trichilemmal tumor arising in a seborrheic keratosis on the buttock and the results of a cell kinetic study of this tumor using a BrdU staining method. The incidence of trichilemmal tumor arising in a seborrheic keratosis seems to be extremely rare.The labeling index of this tumor was 12.0%; this was a level intermediate between normal epidermis and a variety of hyperproliferative skin diseases such as squamous cell carcinoma, Bowen's disease, and psoriasis vulgaris. DNA replicating cells were present in the germinative layers in normal epidermis and the benign hyperproliferative skin diseases, psoriasis vulgaris. In contrast, DNA replicating cells were found throughout the entire epidermis in premalignant and malignant tumors such as in Bowen's disease and squamous cell carcinoma. In this case, DNA replicating cells were localized mainly in the basal and parabasal cell layers, but also seen in the upper squamous layers. These findings suggest that this trichilemmal tumor had a malignant tendency, though it was slow‐growing and relatively benign in nature.
Granulomatous Tissue Reaction Following Cosmetic Eyebrow Tattooing
Authors: Ro Young Suck , Lee Chang Woo 
Published: April 2015

AbstractWe report a case of epithelioid granuloma which occurred at the site of a previous cosmetic eyebrow tattooing. A biopsy specimen showed the organized appearance of epithelioid cell granulomas containing little pigment as well as occasional giant cells, mainly of Langhans' type, thus showing the characteristic features of granulomatous hypersensitivity. With the use of energy dispersive X‐ray microanalysis and inductively coupled plasma spectrometry, the presence of copper, iron, cobalt, and chromium was demonstrated.
Interaction of Human Epidermal Langerhans Cells with HIV‐1 Viral Envelope Proteins (gp 120 and gp 160s) Involves a Receptor‐mediated Endocytosis Independent of the CD4 T4A Epitope
Authors: Dezutter‐Dambuyant Colette , Schmitt Didier A. , Dusserre Nathalie , Hanau Daniel , Kolbe H.V.J. , Kieny Marie‐Paule , Cazenave Jean‐Pierre , Schmitt Daniel , Pasquali Jean‐Louis , Olivier René , Gazzolo Louis , Thivolet Jean 
Published: April 2015

AbstractThe CD4 molecule is known to be the preferential receptor for the HIV‐1 envelope glycoprotein. Epidermal Langerhans cells are dendritic cells which express several surface antigens, among them CD4 antigens. To clarify the exact role of CD4 molecules in Langerhans cell infection induced by HIV‐1, we investigated the possible involvement of the interactions between HIV‐1 gp 120 or HIV‐1 gp 160s (soluble gp 160) and Langerhans cell surface. We also assessed the expression of CD4 molecules on Langerhans cell membranes dissociated by means of trypsin from their neighbouring keratinocytes. The cellular phenotype was monitored using flow cytometry and quantitative immunoelectron microscopy. We reported that human Langerhans cells can bind the viral envelope proteins (gp 120 or gp 160s), and that this binding does not depend on CD4 protein expression. This binding is not blocked by anti‐CD4 monoclonal antibodies. We show that a proportion of gp 120/gp 160s‐receptor complexes enters Langerhans cells by a process identified as a receptor‐mediated endocytosis. The amount of surface bound gp 120/gp 160s is not consistent with the amount of CD4 antigens present on Langerhans cell membranes. Gp 120/gp 160s binding sites on Langerhans cell suspensions appeared to be trypsin resistant, while CD4 antigens (at least the epitopes known to bind the HIV‐1) are trypsin sensitive. A burst of gp 120 receptor expression was detected on 1‐day cultured Langerhans cells while CD4 antigens disappeared. These findings lead to the most logical conclusion that binding of gp 120/gp 160s is due to the presence of a Langerhans cell surface molecule different from CD4 antigens.
Pigmented Macules on the Palms and/or Soles of Japanese Subjects: Influences of Clinical Severity and Duration of Illness
Authors: Narisawa Yutaka , Kohda Hiromu 
Published: April 2015

AbstractWe have suggested that pigmented macules on the palms and/or soles occur in response to various systemic diseases. In the present paper, we evaluated the influences of clinical severity and duration of illness in Japanese subjects. In males, cases who suffered from cerebral vascular accident (CVA) for long periods and/or severely tended to have numerous pigmented macules. In females, cases with CVA did not show any similar tendency. As the result of our prior and present studies, we conclude that underlying systemic disease itself can play a pivotal role in the development of pigmented macules on the palms and/or soles of Japanese subjects. We emphasize that such a pigmented macular eruption with characteristic clinical features is an important sign of internal disorders, including malignancy.
Pigmented Contact Dermatitis Due to Musk Moskene
Authors: Hayakawa Ritsuko , Hirose Osamu , Arima Yaeno 
Published: April 2015

AbstractMusk moskene is a soft, sweet fragrance resembling musk ambrette which introduces a very desirable creamy powder note for cosmetic fragrance. Because of its advantages, which include low cost, oil solubility, and less sensitive to sunlight, musk moskene has recently increased its share of the market.In this paper, we reported a case with pigmented contact dermatitis from musk moskene in cheek rouges. Patch test positive reactions to cheek rouges resulted in hyperpigmentation. Both the perfume used in the cheek rouges and musk moskene, which was a component of that perfume, showed strongly positive reactions. Residual hyperpigmentation was seen on the regions of the perfume and musk moskene patch testings.Hyperpigmentation on cheeks of the patient gradually diminished after discontinuing use of the causative cheek rouge.
Gangliosides Inhibit the Proliferation of Human T Cells Stimulated with Interleukin‐4 or Interleukin‐2
Authors: Morioka Naoko , Furue Masutaka , Tsuchida Tetsuya , Ishibashi Yasumasa 
Published: April 2015

AbstractT cell growth factors such as interleukin‐2 (IL‐2) and interleukin‐4 (IL‐4) act as potent comitogenic factors for purified human T cells in the presence of phorbol myristate acetate (PMA). We investigated the effects of gangliosides on the IL‐4‐driven or IL‐2‐driven proliferation of human T cells, using these comitogenic assays. Bovine brain gangliosides inhibited the proliferation of human T cells activated by PMA + IL‐4 or PMA + IL‐2. These inhibitory effects were dose and time‐dependent and were significant at concentrations higher than 50 μM. PMA + IL‐2 stimulation was more sensitive to the inhibitory effects of gangliosides (I50=77.2 μM) than PMA + IL‐4 stimulation (I50=105.9 μM). Differential inhibitory effects were also examined among the panel of various gangliosides. GD1b and GT1b showed the most potent inhibitory actions in each assay; the inhibitory effect of GD1a was somewhat less potent than GD1b or GT1b. GM2 and GD3 were only weakly inhibitory, and the inhibitory effect of GM3 was almost negligible. These findings suggest that gangliosides may play an immunomodulatory role by interfering with IL‐4 or IL‐2‐driven proliferation of human T cells.
Acneiform Eruption Induced by Lithium Carbonate
Authors: Kanzaki Tamotsu 
Published: April 2015

AbstractA 26‐year‐old female developed a severe acneiform eruption on her face, chest and back soon after she started taking lithium carbonate for psychosis. Histopathological examination revealed it to be folliculitis, rather than true acne. The eruption continued for six months but was resolved three months after discontinuing the drug. It has not reappeard in the following 3 years.
Immunolocalization of Desmoglein I (“Band 3” Polypeptide) on Acantholytic Cells in Pemphigus Vulgaris, Darier's Disease, and Hailey‐Hailey's Disease
Authors: Setoyama Mitsuru , Hashimoto Ken , Tashiro Masaaki 
Published: April 2015

AbstractAcantholysis is defined as loss of coherence between epithelial cells and is histologically shown in several bullous diseases.It was postulated that desmoglein I, one of the major transmembrane glycoproteins of the desmosome, may adhere to the attachment plaque inside the cell and contribute to desmoglea outside the cell.In this study we used a well characterized antibody against desmoglein I for immunofluorescence and immunoelectron microscopic techniques on 2 cases each of pemphigus vulgaris and Darier's disease and one case of Hailey‐Hailey's disease.In the normal epidermis desmosomes were demonstrated in dotted or rim‐like patterns along cell periphery on immunofluorescence study. In pemphigus vulgaris dotted or rim‐like patterns were still identified in many acantholytic cells, particularly in early phase of acantholysis. In Darier's disease and Hailey‐Hailey's disease, dotted or rim‐like patterns were already lost in early acantholysis and immunoreactive desmoglein I proteins were observed diffusely in the cytoplasm. Immunoelectron microscopy confirmed these immunofluorescence observations.It was suggested that in pemphigus vulgaris desmoglein I is unlikely to be the primary site of acantholysis because dotted or rim‐like patterns of immunoreactive desmoglein I are relatively preserved on lesional cells, whereas in genodermatoses such as Darier's disease and Hailey‐Hailey's disease primary abnormalities of desmosomes may be involved in their acantholysis.
Morphological Characterization of Hemangiomatous Tumors Derived from a Novel Murine Vascular Endothelial Cell Line (F‐2)
Authors: Tsujioka Kaoru , Toda Ken‐Ichi , Maruguchi Yukiya , Miyachi Yoshiki , Imamura Sadao 
Published: April 2015

AbstractHemangiomatous tumors were induced in Balb/c nude mice by inoculating F‐2 cells (5 times 106) from a novel tumorigenic murine endothelial cell line which had been established and maintained in our laboratories. These tumors were morphologically investigated in the course of development. Subcutaneous hemorrhage was observed at the inoculation site within 12 hours after the injection of F‐2 cells, followed by development of skin tumors of various sizes at the same sites. They were dome‐shaped, glossy surfaced, black, soft tumors. Mice finally died of massive blood loss due to internal and/or external hemorrhage (on day 10–77). Light microscopically, F‐2 cells formed aggregates immediately after inoculation and then branched into a network of channels and cysts containing erythrocytes. Thereafter, spongiform structures composed of various sizes of cysts appeared with subsequent formation of a single large blood‐filled cyst lined by one or two layers of thin cells. Under an electron microscope, F‐2 cells, possessing large amounts of cytoplasm, formed narrow spaces which were occasionally incomplete with ambiguous basal lamina at the early stages. However, later, in large cysts, they became attenuated, tightly connected, and produced complete lumen surrounded by a basal lamina. Immuno‐histological demonstration of H‐2 K, D antigen showed that tumors induced by F‐2 cells mainly consisted of the inoculated F‐2 cells. These results indicate that F‐2 presents a good experimental system for investigation of vascular endothelial cell tumorigenesis and differentiation.
Penetration of Lysophosphatidylcholine into the Dermis
Authors: Uchida Yoshikazu , Ogawa Tadatomo , Ohta Masao , Kondo Mitsuo , Takada Shigeo , Yamamura Masaichi 
Published: April 2015

AbstractThe ability of Lysophosphatidylcholine (LPC) to penetrate into the dermis and its degradation were investigated in vivo using hairless rats. Phosphatidylcholine (PC) was used as a control. Radioactively labelled LPC and PC were applied on the surface of skin, and the radioactivities of the epidermis and dermis were measured at 8, 24, and 48 hours. The recoveries of radioactive materials of LPC and PC within the area of epidermis and dermis at 8 hours were 0.21% and 0.25%; at 24 hours, 0.68% and 0.31%; and at 48 hours, 0.42% and 0.92%. No radioactivity was detected in serum. The radioactive substances which had penetrated were identified as LPC, PC, diglycerides, and free fatty acids. It was also found that topical application of LPC did not change the structure of skin as seen by microscopical examination.These findings indicate that a small amount of LPC can penetrate without the damaging skin structure and is enzymatically degraded into several lipids. Since LPC has bactericidal and antiviral activity, this substance could be an useful agent for dermatological use.