bluuebunny/crossref_metadata_2025_split huggingface dataset:
Citación telemática y derecho a la defensa de las personas adultas mayores en el ordenamiento jurídico ecuatoriano
Authors: Wong-Vivas Nixon , Cadena-Ramírez Maria 
Published: July 2024

El presente artículo hace un estudio de derechos constitucionales como el derecho a defensa y el debido proceso frente a la citación telemática enfocada a los adultos mayores. La citación telemática se encuentra dada a la reforma del Código Orgánico General de Procesos, publicado mediante Ley s/n, R.O. 245-3S, 7-II-2023, que introduce esta nueva modalidad de citación, la misma que acarrea una serie de vulneraciones a los derechos establecidos en la Constitución de la República del Ecuador hacia los adultos mayores como grupo de atención prioritaria que se encuentren inmersos en un proceso judicial.
La citación telemática establece la construcción de un buzón electrónico ciudadano al cual se les notificará las demandas a las personas naturales. Dicho procedimiento atenta los derechos de los adultos mayores, vulnerando el debido proceso y el derecho a la defensa puesto que la carencia de conocimientos sobre el desarrollo de las nuevas tecnologías y la instauración de buzones electrónicos personales para recibir notificaciones legales, fragmenta la seguridad de permitirles a los adultos mayores ejercer una defensa adecuada por falta de conocimiento de uso de medios electrónicos.
Thromboaspiration of a left-sided bioprosthetic valve thrombosis by a mini-access: the Lausanne novel procedure
Authors: Gunga Ziyad , Rubimbura Vladimir , Oberson Denise , Monney Pierre , Bechtold Xavier , Ltaief Zied , Rancati Valentina , Eeckhout Eric , Kirsch Matthias 
Published: July 2024

Left-sided bioprosthesis valve thrombosis (LSBVT) is a challenging complication necessitating invasive interventions. In this study, we introduce a novel, minimally invasive approach. We used a cerebral embolic protection system and an Occlutech cannula connected to an extracorporeal circuit, providing safer thrombus aspiration compared to the AngioVac system. This technique offers a promising alternative for high-risk patients with LSBVT.
Causal associations between chronic heart failure and the cerebral cortex: results from Mendelian randomization study and integrated bioinformatics analysis
Authors: Peng Liqi , Cai Huzhi , Tang Yanping , Zhou Fang , Liu Yuemei , Xu Zelin , Chen Qingyang , Chen Xinyu 
Published: July 2024

BackgroundChronic heart failure (CHF) patients exhibit alterations in cerebral cortical structure and cognitive function. However, the mechanisms by which CHF affects cortical structure and functional regions remain unknown. This study aims to investigate potential causal relationship between CHF and cerebral cortical structure through Mendelian randomization (MR).MethodsThe research utilized genome-wide association studies (GWAS) to explore the causal association between CHF and cerebral cortical structure. The results were primarily analyzed using the inverse-variance weighted (IVW). The reliability of the data was verified through horizontal pleiotropy and heterogeneity analysis by MR-Egger intercept test and Cochran's Q-test, respectively. Replication analysis was conducted in the Integrative Epidemiology Unit (IEU) OpenGWAS project for further validation. In addition, we collected mediator genes that mediate causality to reveal potential mechanisms. Integrated bioinformatics analysis was conducted using the Open Target Genetics platform, the STRING database, and Cytoscape software.ResultsThe IVW results did not reveal any significant causal association between genetically predicted CHF and the overall structure of the cerebral cortex or the surface area (SA) of the 34 functional regions of the cerebral cortex (P &gt; 0.05). However, the results revealed that CHF increased the thickness (TH) of pars opercularis (IVW: β = 0.015, 95% CI: 0.005–0.025, P = 3.16E-03). Replication analysis supported the causal association between CHF and pars opercularis TH (IVW: β = 0.02, 95% CI: 0.010–0.033, P = 1.84E-04). We examined the degree centrality values of the top 10 mediator genes, namely CDKN1A, CELSR2, NME5, SURF4, PSMA5, TSC1, RPL7A, SURF6, PRDX3, and FTO.ConclusionGenetic evidence indicates a positive correlation between CHF and pars opercularis TH.
Temperature-dependent carbon isotope fractionation in coccolithophores
Authors: Torres-Romero Ismael , Clark Alexander J. , Wijker Reto S. , Jaggi Madalina , Zhang Hongrui , Stoll Heather M. 
Published: July 2024

Introduction: The stable carbon isotope ratio of long-chain alkenones produced by marine haptophyte phytoplankton has often been used to estimate past variations in atmospheric CO2 throughout the Cenozoic. However, previous experimental studies and surveys of alkenones from surface sediment and suspended particulate matter document additional environmental and physiological influences on carbon isotopic fractionation in alkenones.Methods: To clarify the non-CO2 effects on the alkenone carbon isotope fractionations, an important alkenone producer, Gephyrocapsa oceanica, was cultured in laboratory. To separate effects of different environment parameters, G. oceanica was grown in continuous cultures under a matrix of environmental conditions in order to explore the influence of temperature independently of CO2(aq). Through careful manipulation of the media carbon system, we can control the variation of the media CO2(aq) independently of temperature solubility. Carbon isotope fractionations from alkenones, coccolith, and particulate organic carbon were measured from this steady state system.Results and Discussion: We find εp in alkenones and particulate organic carbon inversely correlates with temperature, and temperature affects εp more strongly than CO2(aq). The magnitude of the temperature effect can be explained by higher growth rates at warmer temperatures with a similar growth rate dependence as observed in previous cultures in which growth rate was regulated by other factors. Where the past temperature influence on growth rate could be constrained using the UK’37 alkenone index in the same samples, our finding offers an approach to deconvolve an important physiological factor affecting ancient alkenones εp, and may therefore improve past pCO2 estimates.
Occurrence of 80 per and polyfluorinated alkyl substances (PFAS) in muscle and liver tissues of marine mammals of the St. Lawrence Estuary and Gulf, Quebec, Canada
Authors: Khalid Nejumal Kannankeril , Le Calvez Maud , Lemire Mélanie , Dinh Quoc Tuc , Fontaine Justine , Lair Stéphane , Sauvé Sébastien 
Published: July 2024

Limited data are available for the occurrence of more recent per and polyfluorinated alkyl substances (PFAS) in marine mammals, especially from the St. Lawrence Estuary and Gulf. This study investigates the occurrence of PFAS in liver and muscle tissues of various marine mammals, including the harbor seal, gray seal, harp seal, hooded seal, harbor porpoise, white-sided dolphin, white-beaked dolphin, and True’s beaked whale. Among the 80 target PFAS (including PFCAs, PFSAs, Cyclic PFSA, FASAs/FASAAs, FTCAs/FTUCAs, FTSAs, Ether-PFAS, diPAPs, and ESI + ECF precursors) perfluorooctanoic sulfonate (PFOS) dominates in all the marine mammal species and several other long-chain PFCAs, such as PFNA, PFDA, PFUnA, PFTrDA, and PFHxDA, were detected at 100% frequency in both muscle and liver samples. PFDoA and 7:3 fluorotelomer carboxylic acid (7:3 acid) also showed a 100% detection frequency for liver samples. Harp seal tissues displayed notably low PFAS concentrations, with average total PFAS concentrations of 7 ng/g (ww: wet weight) in muscle and 44 ng/g (ww) in the liver. In contrast, the white-sided dolphin exhibited the highest average concentrations, reaching 39 ng/g (ww) in muscle and 334 ng/g (ww) in liver samples. The Pearson correlation analysis reveals a strong correlation between the concentration of PFOS, perfluoroalkyl carboxylic acids (PFCAs), and electrochemical fluorination (ECF) precursors. Species at the top of the marine food chain (harbor porpoise, white sided dolphin, and white beaked dolphin) presented the highest concentrations of PFAS, particularly PFOS and long-chain PFCAs, highlighting the need for an increased regulation of these persistent molecules in order to protect marine mammal’s health.
Seed enhancements to improve direct-seeding outcomes for native grasses in mine restoration
Authors: Berto Bianca , Ritchie Alison L. , Erickson Todd E. 
Published: July 2024

Seed-based restoration in dryland environments is commonly limited by low and sporadic rainfall, extreme temperatures, and degraded soils. These challenges are exacerbated in dryland mine restoration sites where species are seeded onto mine waste substrates due to limited availability of topsoil. Native grasses are focal dryland and mine restoration species, though the ability to deliver seeds to targeted sites presents further constraints to seed-based restoration due to complex seed morphology and site attributes. Seed enhancement technologies (SETs) may help to mitigate environmental stressors and improving seed handling. In this study, SETs which promote seedling recruitment and/or address edaphic challenges while also improving seed morphology and handling were tested in four Australian native grasses (Cymbopogon ambiguus, C. obtectus, Eulalia aurea, and Eriachne obtusa). Flash flaming or acid digestion (depending on species), extruded seed pellets with or without the addition of topsoil, and flaming or acid digestion used in combination with extruded pellets (with or without topsoil) were evaluated on topsoil and mine waste substrates. Material treated with either flash flaming or acid digestion tended to produce similar maximum emergence (week 3), final emergence (week 14), and above-ground biomass (week 14) on both topsoil and mine waste when compared to the un-enhanced (‘control’) treatment. Extruded pellets enhanced emergence (maximum and final) on both soil types, though had no effect on biomass. Extruded pellets containing topsoil and/or when used in combination with flash flamed or acid digested florets, provided the most significant benefits to seedling emergence, particularly on mine waste soil. However, SETs were unable to mitigate the detrimental effects of mine waste substrates on seedling biomass. This study shows promise for improving seed-based restoration in post-mine and dryland environments using SETs, though also highlights potential limitations and the importance of understanding long-term (i.e., beyond emergence) plant recruitment outcomes in SET applications.
Single cell multi-omic analysis identifies key genes differentially expressed in innate lymphoid cells from COVID-19 patients
Authors: Kaushik Abhinav , Chang Iris , Han Xiaorui , He Ziyuan , Komlosi Zsolt I. , Ji Xuhuai , Cao Shu , Akdis Cezmi A. , Boyd Scott , Pulendran Bali , Maecker Holden T. , Davis Mark M. , Chinthrajah R. Sharon , DeKruyff Rosemarie H. , Nadeau Kari C. 
Published: July 2024

IntroductionInnate lymphoid cells (ILCs) are enriched at mucosal surfaces where they respond rapidly to environmental stimuli and contribute to both tissue inflammation and healing. MethodsTo gain insight into the role of ILCs in the pathology and recovery from COVID-19 infection, we employed a multi-omics approach consisting of Abseq and targeted mRNA sequencing to respectively probe the surface marker expression, transcriptional profile and heterogeneity of ILCs in peripheral blood of patients with COVID-19 compared with healthy controls. ResultsWe found that the frequency of ILC1 and ILC2 cells was significantly increased in COVID-19 patients. Moreover, all ILC subsets displayed a significantly higher frequency of CD69-expressing cells, indicating a heightened state of activation. ILC2s from COVID-19 patients had the highest number of significantly differentially expressed (DE) genes. The most notable genes DE in COVID-19 vs healthy participants included a) genes associated with responses to virus infections and b) genes that support ILC self-proliferation, activation and homeostasis. In addition, differential gene regulatory network analysis revealed ILC-specific regulons and their interactions driving the differential gene expression in each ILC. DiscussionOverall, this study provides mechanistic insights into the characteristics of ILC subsets activated during COVID-19 infection.
Precision therapy for ulcerative colitis: insights from mitochondrial dysfunction interacting with the immune microenvironment
Authors: Zhang Yi-fan , Fan Meng-ying , Bai Qi-rui , Zhao Rong , Song Shan , Wu Li , Lu Jun-hui , Liu Jing-wei , Wang Qi , Li Yuan , Chen Xing 
Published: July 2024

BackgroundAccumulating evidence reveals mitochondrial dysfunction exacerbates intestinal barrier dysfunction and inflammation. Despite the growing knowledge of mitochondrial dysfunction and ulcerative colitis (UC), the mechanism of mitochondrial dysfunction in UC remains to be fully explored.MethodsWe integrated 1137 UC colon mucosal samples from 12 multicenter cohorts worldwide to create a normalized compendium. Differentially expressed mitochondria-related genes (DE-MiRGs) in individuals with UC were identified using the “Limma” R package. Unsupervised consensus clustering was utilized to determine the intrinsic subtypes of UC driven by DE-MiRGs. Weighted gene co-expression network analysis was employed to investigate module genes related to UC. Four machine learning algorithms were utilized for screening DE-MiRGs in UC and construct MiRGs diagnostic models. The models were developed utilizing the over-sampled training cohort, followed by validation in both the internal test cohort and the external validation cohort. Immune cell infiltration was assessed using the Xcell and CIBERSORT algorithms, while potential biological mechanisms were explored through GSVA and GSEA algorithms. Hub genes were selected using the PPI network.ResultsThe study identified 108 DE-MiRGs in the colonic mucosa of patients with UC compared to healthy controls, showing significant enrichment in pathways associated with mitochondrial metabolism and inflammation. The MiRGs diagnostic models for UC were constructed based on 17 signature genes identified through various machine learning algorithms, demonstrated excellent predictive capabilities. Utilizing the identified DE-MiRGs from the normalized compendium, 941 patients with UC were stratified into three subtypes characterized by distinct cellular and molecular profiles. Specifically, the metabolic subtype demonstrated enrichment in epithelial cells, the immune-inflamed subtype displayed high enrichment in antigen-presenting cells and pathways related to pro-inflammatory activation, and the transitional subtype exhibited moderate activation across all signaling pathways. Importantly, the immune-inflamed subtype exhibited a stronger correlation with superior response to four biologics: infliximab, ustekinumab, vedolizumab, and golimumab compared to the metabolic subtype.ConclusionThis analysis unveils the interplay between mitochondrial dysfunction and the immune microenvironment in UC, thereby offering novel perspectives on the potential pathogenesis of UC and precision treatment of UC patients, and identifying new therapeutic targets.
Relationship between dietary protein, serum albumin, and mortality in asthmatic populations: a cohort study
Authors: Zhuang Rongjuan , Liao Jiaxin , Giri Mohan , Wen Jun , Guo Shuliang 
Published: July 2024

BackgroundCurrently, there is limited research on the correlation between protein levels in the body and asthma. We used data from the NHANES to explore the relationship of dietary protein, serum albumin, with mortality in individuals with asthma to better understand their impact on asthma.MethodThis investigation involved 3005 individuals with asthma from the NHANES dataset. Studying potential links between dietary protein, serum albumin, and mortality in asthmatic populations utilized the Cox proportional hazards models, trend test, restricted cubic splines (RCS), and Kaplan-Meier survival analysis. Furthermore, subgroup analyses were carried out to explore these connections within specific populations.ResultAfter considering all potential variables, multivariate Cox proportional hazard models proved that dietary protein intake did not have an independent connection with all-cause mortality, but serum albumin was inversely linked with all-cause mortality. Each unit rise in serum albumin (g/l) was linked to a 13% decrease in the likelihood of all-cause mortality. RCS confirmed a negative and linear connection of serum albumin with all-cause mortality. The Kaplan-Meier survival curve suggested that asthmatic adults with greater serum albumin levels had a decreased risk of mortality compared to those with lower levels.ConclusionThe investigation proved a negative linear connection of serum albumin with all-cause mortality in asthma patients. However, there was no independent link discovered between dietary protein intake with mortality. This indicates that serum albumin could be a significant factor in predicting long-term outcomes for asthma patients.
Unraveling the role of Major Vault Protein as a novel immune-related biomarker that promotes the proliferation and migration in pancreatic adenocarcinoma
Authors: Wu Xinyi , Hao Leiyu , Lin Jianghua , Guo Xinyu , Luo Yuping , Li Chun 
Published: July 2024

BackgroundPancreatic adenocarcinoma (PAAD) is a formidable challenge in oncology research, with a complex pathogenesis that requires to be explored. Major Vault Protein (MVP) is the principal structural component of the vault complex, and its expression level is remarkably upregulated in various cancers. Extensive investigations have been conducted to explore the role of MVP in specific cancer contexts, yet the potential molecular mechanisms and biological functions of MVP in PAAD still remain considerably elusive. This study aims to explore the role of MVP as a novel immune-related biomarker in the pathogenesis and clinical treatment of PAAD.MethodsGene expression data and clinical information were collected from TCGA, GTEx and GEO databases. Survival, prognostic and functional enrichment analysis were employed with R software. Immunological correlation analysis was performed using TIMER2.0, TIDE scores, TISIDB and TISCH. Epigenetic analysis was implemented by MethSurv, CPTAC, UALCAN, and cBioPortal. Drug analysis was conducted using Enrichr and CellMiner. Moreover, cellular experiments, like RNA interference, qRT-PCR, Western blot, cell cycle analysis, cell apoptosis analysis, colony formation assay, transwell assay, and wound healing assay, were performed for verifying the functional properties of MVP in the PAAD progression.ResultsWe demonstrated an abnormally upregulated expression of MVP in PAAD tissues, which notably correlated with an adverse prognosis in PAAD patients. Functional analysis suggested the conceivable involvement of MVP in immune modulation, and immunotherapy. Additionally, we identified genetic alterations, reduced promoter methylation, and heightened phosphorylation in MVP. We also clarified Suloctidil and Tetradioxin as the most notable potential drugs targeting MVP in PAAD. Moreover, our experimental observations consistently highlighted the significant impact of MVP deficiency on impeding PAAD cell proliferation, inhibiting cell migration, and accelerating cell apoptosis. Interestingly, a potential link between MVP and ERK or AKT pathways was displayed, which opens new avenues for further exploration of the molecular mechanisms of MVP-targeted therapies in PAAD.ConclusionsThis study systematically describes MVP as an immune-related biomarker with remarkable potential for predicting the prognosis, tumor progression and immunotherapeutic efficacy in PAAD.
Plasma proteins and psoriatic arthritis: a proteome-wide Mendelian randomization study
Authors: Zhao Heran , Zhou Yi , Wang Ziyan , Zhang Xuan , Chen Leilei , Hong Zhinan 
Published: July 2024

BackgroundPrevious epidemiological studies have identified a correlation between serum protein levels and Psoriatic Arthritis (PsA). However, the precise nature of this relationship remains uncertain. Therefore, our objective was to assess whether circulating levels of 2,923 plasma proteins are associated with the risk of PsA, utilizing the Mendelian randomization (MR) approach.MethodsTwo-sample MR analysis was performed to assess the causal impact of proteins on PsA risk. Exposure data for plasma proteins were sourced from a genome-wide association study (GWAS) conducted within the UK Biobank Pharma Proteomics Project, which encompassed 2,923 unique plasma proteins. The outcome data for PsA were sourced from the FinnGen study, a large-scale genomics initiative, comprising 3,537 cases and 262,844 controls. Additionally, colocalization analysis, Phenome-wide MR analysis, and candidate drug prediction were employed to identify potential causal circulating proteins and novel drug targets.ResultsWe thoroughly assessed the association between 1,837 plasma proteins and PsA risk, identifying seven proteins associated with PsA risk. An inverse association of Interleukin-10 (IL-10) with PsA risk was observed [odds ratio (OR)=0.45, 95% confidence interval (CI), 0.28 to 0.70, PFDR=0.072]. Additionally, Apolipoprotein F (APOF) has a positive effect on PsA risk (OR=2.08, 95% CI, 1.51 to 2.86, PFDR=0.005). Subsequently, we found strong evidence indicating that IL-10 and APOF were colocalized with PsA associations (PP.H4 = 0.834 for IL-10 and PP.H4 = 0.900 for APOF). Phenome-wide association analysis suggested that these two proteins may have dual effects on other clinical traits (PFDR&lt;0.1)ConclusionThis study identified 7 plasma proteins associated with PsA risk, particularly IL-10 and APOF, which offer new insights into its etiology. Further studies are needed to assess the utility and effectiveness of these candidate proteins.
Case report: A huge retroperitoneal solitary fibrous tumor closely related to the external iliac vessels misdiagnosed as an ovarian tumor
Authors: Zhang Danni , Wang Li , Zhang Lili , Yao Shuzhi , Wu Juntong , Han Song 
Published: July 2024

BackgroundSolitary fibrous tumor (SFT) is a rare soft tissue tumor originating from mesenchymal cells. Thus far, there have been no reported cases of SFT closely related to the iliac vessels.Case presentationAn elderly woman was found to have had a lower abdominal mass for more than 20 years. The enhanced computerized tomography (CT) showed a progressively enhanced hypervascular mass. The external iliac blood vessels were closely related to the mass, which was misdiagnosed as an ovarian tumor. During laparotomy, the external iliac vein was seen to penetrate the tumor, and the external iliac artery was seen to penetrate the tumor capsule. The retroperitoneal tumor was diagnosed during the operation. The surgical plan of complete tumor resection, severing of the external iliac arteries and veins, and blood vessel replacement was implemented. Pathological immunohistochemistry showed positive results for STAT6 and CD34, confirming the diagnosis of giant retroperitoneal SFT. The risk is classified as high and requires long-term follow-up. There has been no local recurrence or distant metastasis almost 1 year after surgery.ConclusionThe incidence of giant retroperitoneal SFT is rare, and the diagnosis can be confirmed through preoperative imaging examination and pathological examination. If the SFT capsule is intact, there is a chance of surgical resection. For SFTs that are penetrated by the iliac blood vessels, adequate preparation must be made before the surgery is performed. Removing the tumor and the iliac blood vessels at the corresponding site and then replacing it with artificial blood vessels is a feasible method with less risk of bleeding. In this case, imaging showed a progressively enhancing hypervascular mass in the lower abdomen, which was related to blood vessels. Preoperative biopsy and pathological testing can confirm the diagnosis. Neoadjuvant therapy or interventional therapy before surgery can shrink the tumor, making the surgical procedure relatively easy with less risk of bleeding.