bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gut microbiota dysbiosis links chronic apical periodontitis to liver fibrosis in nonalcoholic fatty liver disease: Insights from a mouse model
Authors: Gan Guowu , Luo Yufang , Zeng Yu , Lin Shihan , Lu Beibei , Zhang Ren , Chen Shuai , Lei Huaxiang , Cai Zhiyu , Huang Xiaojing 
Published: July 2024

AbstractAimIn this study, we investigated the systemic implications of chronic apical periodontitis (CAP). CAP may contribute to the nonalcoholic fatty liver disease (NAFLD) progression through the gut microbiota and its metabolites, which are related to the degree of fibrosis.MethodologySixteen 7‐week‐old male apolipoprotein E knockout (apoE−/−) mice were randomly divided into two groups: the CAP and Con groups. A CAP model was established by sealing the first‐ and second‐maxillary molars with bacterium‐containing cotton balls. Apical lesions were evaluated by micro‐CT. Histological evaluations of NAFLD were performed using second harmonic generation/two‐photon excitation fluorescence (SHG/TPEF) assays. Additionally, we comprehensively analyzed the gut microbiota using 16S rRNA gene sequencing and explored metabolic profiles by liquid chromatography–mass spectrometry (LC–MS). Immunofluorescence analysis was used to examine the impact of CAP on tight junction proteins and mucin expression. Transcriptome assays have elucidated gene expression alterations in liver tissues.ResultsMicro‐CT scans revealed an evident periapical bone loss in the CAP group, and the total collagen percentage was increased (Con, 0.0361 ± 0.00510%, CAP, 0.0589 ± 0.00731%, p < .05). 16S rRNA sequencing revealed reduced diversity and distinct taxonomic enrichment in the CAP group. Metabolomic assessments revealed that differentially enriched metabolites, including D‐galactosamine, were enriched and that 16‐hydroxyhexadecanoic acid and 3‐methylindole were depleted in the CAP group. Immunofluorescence analyses revealed disruptions in tight junction proteins and mucin production, indicating intestinal barrier integrity disruption. Liver transcriptome analysis revealed upregulation of Lpin‐1 expression in the CAP group.ConclusionThis study provides comprehensive evidence of the systemic effects of CAP on liver fibrosis in NAFLD patients by elucidating alterations in the gut microbiota composition and metabolism.
Urinary tract infections in patients with urinary catheterization receiving home health service: A prevalence study
Authors: Köse Mert , Çiftçi Bahar 
Published: July 2024

AbstractThis study aimed to determine the prevalence of urinary tract infections in patients with urinary catheterization receiving home health services and the affecting factors. The population of this descriptive study consisted of patients who had lived in the central districts of Erzurum between February and March 2022, were actively registered to home health services, had a urinary catheter and met the research criteria. The period prevalence method (3 months) was used in the study, and 121 patients constituted the study sample. The study data were collected using a Sociodemographic and Information Form on Urinary Catheter. Before the home visit, patients' relatives were asked to clamp the urinary catheter. After completing the data collection forms, a sufficient amount of urine was taken from the attached urinary catheter, put into the urine and culture cup/tube, and labelled with a barcode. The samples were sent to the laboratory within 15 min at the latest. The medical specialist evaluated the results, and the necessary pharmacological treatment was delivered to the patient. Of the patients with indwelling urinary catheters who received home health services, 94.2% had a urinary tract infection. Moreover, it was determined that there was a statistically significant correlation between the presence of urinary tract infection in patients and the variables of constipation, frequency of perineal cleaning, and the use of toilet paper. It was concluded that the prevalence of urinary tract infections is very high in patients with urinary catheterization receiving home health services.
Recent advances in understanding of the mechanisms of RNA interference in insects
Authors: Koo Jinmo , Palli Subba Reddy 
Published: July 2024

AbstractWe highlight the recent 5 years of research that contributed to our understanding of the mechanisms of RNA interference (RNAi) in insects. Since its first discovery, RNAi has contributed enormously as a reverse genetic tool for functional genomic studies. RNAi is also being used in therapeutics, as well as agricultural crop and livestock production and protection. Yet, for the wider application of RNAi, improvement of its potency and delivery technologies is needed. A mechanistic understanding of every step of RNAi, from cellular uptake of RNAi trigger molecules to targeted mRNA degradation, is key for developing an efficient strategy to improve RNAi technology. Insects provide an excellent model for studying the mechanism of RNAi due to species‐specific variations in RNAi efficiency. This allows us to perform comparative studies in insect species with different RNAi sensitivity. Understanding the mechanisms of RNAi in different insects can lead to the development of better strategies to improve RNAi and its application to manage agriculturally and medically important insects.
Genome‐wide association and polygenic risk score estimation of type 2 diabetes mellitus in Kinh Vietnamese—A pilot study
Authors: Mai Thao Phuong , Luong Bac An , Ma Phat Tung , Tran Thang Viet , Dinh Ngo Tat Thang , Hoang Chi Khanh , Van Tran Luong , Le Bao Hoang , Vu Hoang Anh , Le Linh Hoang Gia , Le Khuong Thai , Truong Steven , Tran Nam Quang , Do Minh Duc 
Published: July 2024

AbstractA genome‐wide association study (GWAS) is a powerful tool in investigating genetic contribution, which is a crucial factor in the development of complex multifactorial diseases, such as type 2 diabetes mellitus. Type 2 diabetes mellitus is a major healthcare burden in the Western Pacific region; however, there is limited availability of genetic‐associated data for type 2 diabetes in Southeast Asia, especially among the Kinh Vietnamese population. This lack of information exacerbates global healthcare disparities. In this study, 997 Kinh Vietnamese individuals (503 with type 2 diabetes and 494 controls) were prospectively recruited and their clinical and paraclinical information was recorded. DNA samples were collected and whole genome genotyping was performed. Standard quality control and genetic imputation using the 1000 Genomes database were executed. A polygenic risk score for type 2 diabetes was generated in different models using East Asian, European, and mix ancestry GWAS summary statistics as training datasets. After quality control and genetic imputation, 107 polymorphisms reached suggestive statistical significance for GWAS (≤5 × 10−6) and rs11079784 was one of the potential markers strongly associated with type 2 diabetes in the studied population. The best polygenic risk score model predicting type 2 diabetes mellitus had AUC = 0.70 (95% confidence interval = 0.62–0.77) based on a mix of ancestral GWAS summary statistics. These data show promising results for genetic association with a polygenic risk score estimation in the Kinh Vietnamese population; the results also highlight the essential role of population diversity in a GWAS of type 2 diabetes mellitus.
Hematology and serum biochemistry of free‐ranging mantled howler monkeys (Alouatta palliata) at La Pacifica, Costa Rica
Authors: Larsen R. Scott , Moresco Anneke , Meneses Ana , Glander Kenneth E. 
Published: July 2024

AbstractBackgroundHematologic and blood biochemical values are key tools for assessing primate health. A long‐term behavioral study of howler monkeys at a single site (La Pacífica, Guanacaste, Costa Rica), afforded the opportunity to develop baseline values for a large group of animals, evaluating differences between adult males and females and comparing to a report in the same population two decades later.MethodsIn 1998, 64 free‐ranging mantled howler monkeys were anesthetized and sampled for hematologic and biochemical analysis.ResultsBlood analysis is reported for 29 adult females, 9 juvenile females, 19 adult males and 3 juvenile males. Four adults were excluded due to external injury or disease. There were few significant differences between adult females, juvenile females, and adult males.ConclusionsBaseline blood parameters are useful for determining normal values for howler monkey populations. The values for total protein, blood urea nitrogen, glucose, liver enzymes and potassium differed from a later study in 2019 may indicate changes that are influencing howler monkey health.
Turning down employee voice with humour: A mixed blessing for employee voice resilience?
Authors: Hamstra Melvyn R. W. , Guzman Felipe A. , Qian Si , Schreurs Bert , Jawahar I. M. (Jim) 
Published: July 2024

AbstractGiven that not all suggestions can be implemented, understanding how supervisors can turn down employee voiced suggestions while not discouraging employees voicing in the future is critical for theoretical and practical reasons. Supervisors may use humour when not endorsing employees' suggestions as they attempt to ease tension by injecting something lighthearted, but doing so, we argue, is not uniformly beneficial. Hence, we conducted a preregistered study that tests how supervisors' use of humour when turning down an employee's voiced suggestion affects voice resilience. Utilizing signaling theory, we theorize supervisors' use of humour when turning down voice strengthens voice safety but weakens voice impact perceptions. Indirectly, humour therefore may constitute a mixed blessing for voice resilience (voice behaviour after voice non‐endorsement). Additionally, we hypothesized that the positive link between humour and voice safety and the negative link between humour and voice impact are moderated by supervisor–employee relationship quality (leader–member exchange (LMX)). We tested these predictions in a time‐lagged study of 343 employees whose voice was recently turned down. Humour indeed increased voice resilience via voice safety; against expectations, humour positively related to voice impact (via it, resilience). LMX is significantly moderated. However, unexpectedly, humour helped voice safety, impact and the resilience of low LMX employees.
Current understanding and new insights in the treatment of IgA nephropathy
Authors: Zhang Yuemiao , Zhang Hong 
Published: July 2024

AbstractIgA nephropathy (IgAN) is the most common primary glomerulonephritis worldwide, and almost all patients are at risk of progression to end‐stage kidney disease within their lifetime. The mechanisms responsible for the presentation and development of IgAN are required for the development of highly targeted therapies for this disease. In this review, we first demonstrate the current treatment strategy of IgAN recommended by the 2021 KDIGO guideline. Then, we update the new insights into disease pathogenesis based on the well acknowledged ‘multiple‐hit hypothesis’ and provide the potential therapeutic targets involved in the upstream production of pathogenic IgA1 and the downstream complement activation. Finally, the recent large randomized controlled trials focusing on these novel targets have been summarized, among which Nefecon and Sparsentan have received approval and Telitacicept have been used off‐label for IgAN. In the future, emerging treatment approaches for IgAN is likely to evolve, which will signify a shift in the management of the IgAN from traditional immunosuppressive approaches to an era of targeted treatment based on the understanding of the pathogenic mechanisms.
Comparative analyses of the information content of letters, characters, and inter‐word spaces across writing systems
Authors: Huang Linjieqiong , Reichle Erik D. , Li Xingshan 
Published: July 2024

AbstractOne difference among writing systems is how orthographic cues are used to demarcate words; although most alphabetic scripts use inter‐word spaces, some Asian scripts do not explicitly mark word boundaries (e.g., Chinese). It is unclear whether these differences are arbitrary or whether they are designed to maximize reading efficiency. Here, we show that spaces inserted between words in non‐demarcated scripts provide less information about word boundaries than spaces in demarcated scripts. Furthermore, despite the fact that less information is contained by inter‐word spaces than characters/letters of the same size, the information content of inter‐word spaces in demarcated scripts is closer to that of characters/letters compared to the information content of inter‐word spaces that are inserted in non‐demarcated scripts. These results suggest that the conventions used to demarcate word boundaries are sufficient to support efficient reading. Our findings provide new insights into the universals and variation across writing systems and shed light on the mental processes that support skilled reading.
Chinese Import Competition and Prices: Evidence from India*
Authors: Chakraborty Pavel , Henry Michael , Singh Rahul 
Published: July 2024

How do output prices respond to trade shocks? Using detailed firm‐product level data on sales and quantity between 1996 and 2007, we study the causal effect of Chinese import competition on prices for Indian manufacturing firms. We find that Chinese import competition induces a significant decline in firm‐product prices. A 1 percentage point increase in the Chinese import penetration ratio reduces firm‐product prices by 3.5%. Further, this decline in prices is driven by a decline in markup, conditional on costs, as opposed to the pass through of cost savings to prices – providing evidence for pro‐competitive effect. This decline in prices and markup is less pronounced for firms owned by Business Groups compared to stand‐alone, privately owned firms. We also document a large decrease in marginal costs and an increase in markup with no significant effect on prices for firms on account of increased access to imported Chinese inputs.
Pyramiding of triple Clubroot resistance loci conferred superior resistance without negative effects on agronomic traits in Brassica napus
Authors: Baloch Amanullah , Shah Nadil , Idrees Fahad , Zhou Xueqing , Gan Longcai , Atem Jalal Eldeen Chol , Zhou Yuanwei , Piao Zhongyun , Chen Peng , Zhan Zongxiang , Zhang Chunyu 
Published: July 2024

AbstractClubroot disease caused by Plasmodiophora brassicae is becoming a serious threat to rapeseed (Brassica napus) production worldwide. Breeding resistant varieties using CR (clubroot resistance) loci is the most promising solution. Using marker‐assisted selection and speed‐breeding technologies, we generated Brassica napus materials in homozygous or heterozygous states using CRA3.7, CRA08.1, and CRA3.2 loci in the elite parental line of the Zhongshuang11 background. We developed three elite lines with two CR loci in different combinations and one line with three CR loci at the homozygous state. In our study, we used six different clubroot strains (Xinmin, Lincang, Yuxi, Chengdu, Chongqing, and Jixi) which are categorized into three groups based on our screening results. The newly pyramided lines with two or more CR loci displayed better disease resistance than the parental lines carrying single CR loci. There is an obvious gene dosage effect between CR loci and disease resistance levels. For example, pyramided lines with triple CR loci in the homozygous state showed superior resistance for all pathogens tested. Moreover, CR loci in the homozygous state are better on disease resistance than the heterozygous state. More importantly, no negative effect was observed on agronomic traits for the presence of multiple CR loci in the same background. Overall, these data suggest that the pyramiding of triple clubroot resistance loci conferred superior resistance with no negative effects on agronomic traits in Brassica napus.
Seasonal plasticity of stem embolism resistance and its potential driving factors in six temperate woody species
Authors: Ma Bo‐Long , Liao Su‐Hui , Lv Qing‐Zi , Huang Xin , Jiang Zai‐Min , Cai Jing 
Published: July 2024

AbstractThe seasonal plasticity of resistance to xylem embolism has been demonstrated in leaves of some tree species, but is controversial in stems. In this study, we investigated the seasonality of stem xylem resistance to embolism in six temperate woody species (four deciduous and two evergreen tree species) that were grown at the same site. The xylem conduit anatomy, the concentrations, and ratios of the main cation in the xylem sap, as well as the content of nonstructural carbohydrates (including soluble sugars and starch) were measured in each species under each season to reveal the potential mechanisms of seasonal change in embolism resistance. The stem of all species showed increasing resistance to embolism as seasons progressed, with more vulnerable xylem in spring, but no significant adjustment in the other three seasons. The seasonal plasticity of stem embolism resistance was greater in deciduous species than in evergreen. On a seasonal scale, conduit diameter and conduit implosion resistance, the ratios of K+/Ca2+ and K+/Na+, and starch content were generally not correlated with embolism resistance, suggesting that these are probably not the main drivers of seasonal plasticity of stem embolism resistance. The seasonality of embolism resistance provides critical information for better understanding plant hydraulics in response to seasonal environments, especially under climate change.
Lancaster correlation: A new dependence measure linked to maximum correlation
Authors: Holzmann Hajo , Klar Bernhard 
Published: July 2024

AbstractWe suggest novel correlation coefficients which equal the maximum correlation for a class of bivariate Lancaster distributions while being only slightly smaller than maximum correlation for a variety of further bivariate distributions. In contrast to maximum correlation, however, our correlation coefficients allow for rank and moment‐based estimators which are simple to compute and have tractable asymptotic distributions. Confidence intervals resulting from these asymptotic approximations and the covariance bootstrap show good finite‐sample coverage. In a simulation, the power of asymptotic as well as permutation tests for independence based on our correlation measures compares favorably with competing methods based on distance correlation or rank coefficients for functional dependence, among others. Moreover, for the bivariate normal distribution, our correlation coefficients equal the absolute value of the Pearson correlation, an attractive feature for practitioners which is not shared by various competitors. We illustrate the practical usefulness of our methods in applications to two real data sets.
Pre‐service middle school teachers' specialized content knowledge on sampling variability
Authors: Abu‐Ghalyoun Omar , Al‐Abed Adnan 
Published: July 2024

AbstractThis study investigates a range of non‐normative ideas that pre‐service teachers (PSTs) employ in reasoning about sampling variability. This issue was studied in the context of a content course on statistics and probability for pre‐service middle grade teachers at a Midwestern American university. Analysis of seven PSTs' video and screen records of task‐based interviews has articulated fundamental facets of sampling variability that have not yet been fully explicated in the literature, especially with middle grade PSTs. With the content expectation of sampling variability for middle grade students as suggested by policy reports in the United States of America, this study is particularly fertile ground for designing curricula that can support middle grade PSTs' development of critical specialized content knowledge on sampling variability.
Rove beetles occurred widely along the Pacific coasts of the Northern Hemisphere: Phylogeny and biogeography of the marine littoral Psammostiba (Coleoptera, Staphylinidae)
Authors: Song Jeong‐Hun , Jung Jong‐Keun , Ahn Kee‐Jeong 
Published: July 2024

AbstractThe North Pacific distribution of coastal aleocharines has been explained as the result of either dispersal or vicariance. The rove beetle genus Psammostiba is a marine littoral group that occurs on the Pacific coasts of the Northern Hemisphere. We performed phylogenetic analysis of Psammostiba using molecular characters (4685 bp) to investigate their biogeographic patterns. The data were analysed using parsimony, Bayesian and maximum likelihood methods. Model‐based analyses showed the same pattern of Psammostiba species relationships, but parsimony analysis yielded different species relationships for the unresolved clade of Psammostiba. According to the reconstruction of the ancestral areas, both vicariance (two events) and dispersal (one event) have played roles in shaping its current distribution. Biogeographical analyses suggest that the common ancestor of Psammostiba occurred widely along the Pacific coasts of the Northern Hemisphere and underwent vicariance events.
Optimal Implant Positioning Following Total Knee Arthroplasty Using Predictive Dynamic Simulation
Authors: Danaei Behzad , McPhee John 
Published: July 2024

Abstract
In this paper, a novel method is proposed for the determination of the optimal subject-specific placement of knee implants based on predictive dynamic simulations of human movement following total knee arthroplasty (TKA). Two knee implant models are introduced. The first model is a comprehensive 12-degree-of-freedom (DoF) representation that incorporates volumetric contact between femoral and tibial implants, as well as patellofemoral contact. The second model employs a single-degree-of-freedom equivalent kinematic (SEK) approach for the knee joint. A cosimulation framework is proposed to leverage both knee models in our simulations. The knee model is calibrated and validated using patient-specific data, including knee kinematics and ground reaction forces. Additionally, quantitative indices are introduced to evaluate the optimality of implant positioning based on three criteria: balancing medial and lateral load distributions, ligament balancing, and varus/valgus alignment. The knee implant placement is optimized by minimizing the deviation of the indices from their user-defined desired values during predicted sit-to-stand motion. The method presented in this paper has the potential to enhance the results of knee arthroplasty and serve as a valuable instrument for surgeons when planning and performing this procedure.