bluuebunny/crossref_metadata_2025_split huggingface dataset:
Innovations in Practice: Dissemination and implementation of child–parent psychotherapy in rural public health agencies
Authors: Barnett Erin R. , Rosenberg Harriet J. , Rosenberg Stanley D. , Osofsky Joy D. , Wolford George L. 
Published: October 2013

BackgroundEarly trauma exposure can have long‐term negative health effects. Few young children receive evidence‐based trauma treatment. This article explores the feasibility of implementing Child–Parent Psychotherapy (CPP), an evidence‐based intervention, in rural public health agencies.MethodTwenty‐three clinicians across four agencies were trained. Training outcomes and implementation barriers and facilitators were assessed.ResultsOne hundred twelve client–caregiver dyads began the year‐long treatment; 50% are currently enrolled or have completed treatment. Barriers and facilitators to implementation were identified.ConclusionsCPP is feasible to implement in rural community mental health agencies. Important lessons were learned related to planning, implementation, and sustainability.
A New i, i + 3 Peptide Stapling System for α‐Helix Stabilization
Authors: Shim So Youn , Kim Young‐Woo , Verdine Gregory L. 
Published: October 2013

We have previously shown that the incorporation of an 8‐atom all‐hydrocarbon ‘staple’ at positions i and i + 3 of a synthetic peptide results in substantial stabilization of the α‐helical conformation. As part of our ongoing effort to explore the scope and utility of all‐hydrocarbon stapling systems, we have investigated and report herein the properties of a new i, i + 3 stapling system that employs a 6‐carbon cross‐link.
Gsp mutation in acromegaly and its influence on TRH‐induced paradoxical GH response
Authors: Goto Yuko , Kinoshita Manabu , Oshino Satoru , Arita Hideyuki , Kitamura Tetsuhiro , Otsuki Michio , Shimomura Iichiro , Yoshimine Toshiki , Saitoh Youichi 
Published: October 2013

SummaryObjectiveWe recently reported that paradoxical GH response to TRH administration reflects biological characteristics in patients with acromegaly. The aim of this study is to elucidate the relationship between gsp mutations and the paradoxical GH response to TRH.PatientsSixty‐seven patients with acromegaly were included for analysis. Paradoxical increase in serum GH level to TRH, GH suppression by octreotide and bromocriptine, radiological profiles and histopathological findings were analysed with respect to tumour gsp‐mutation status.ResultsTwenty‐six (38·8%) gsp mutations were detected, and the number of paradoxical GH responders to TRH, defined as an increase of 100% or more in GH after TRH, was 49 (73·1%). Among the paradoxical GH responders to TRH, 21 patients (42·9%) had a gsp mutation and 28 patients (57·1%) did not. The percentage of paradoxical GH responders to TRH in gsp‐positive and gsp‐negative patients was not significantly different (80·8% and 68·3%, respectively). The gsp‐positive group showed a significantly higher paradoxical increase in serum GH level by TRH administration (1830% vs 650% GH increase, P = 0·045) and greater GH suppression by octreotide (88·7% vs 75·4% GH decrease, P = 0·003) than the gsp‐negative group.ConclusionParadoxical GH response to TRH was observed regardless of gsp mutation, although the rate of increase was significantly higher in gsp‐positive patients. These results suggest that gsp mutation is not sufficient to cause the paradoxical GH response to TRH, while other unidentified factors have a strong influence on paradoxical GH response to TRH in patients with acromegaly.
A Framework for Developing Objective and Measurable Recovery Criteria for Threatened and Endangered Species
Authors: HIMES BOOR GINA K. 
Published: October 2013

AbstractFor species listed under the U.S. Endangered Species Act (ESA), the U.S. Fish and Wildlife Service and National Marine Fisheries Service are tasked with writing recovery plans that include “objective, measurable criteria” that define when a species is no longer at risk of extinction, but neither the act itself nor agency guidelines provide an explicit definition of objective, measurable criteria. Past reviews of recovery plans, including one published in 2012, show that many criteria lack quantitative metrics with clear biological rationale and are not meeting the measureable and objective mandate. I reviewed how objective, measureable criteria have been defined implicitly and explicitly in peer‐reviewed literature, the ESA, other U.S. statutes, and legal decisions. Based on a synthesis of these sources, I propose the following 6 standards be used as minimum requirements for objective, measurable criteria: contain a quantitative threshold with calculable units, stipulate a timeframe over which they must be met, explicitly define the spatial extent or population to which they apply, specify a sampling procedure that includes sample size, specify a statistical significance level, and include justification by providing scientific evidence that the criteria define a species whose extinction risk has been reduced to the desired level. To meet these 6 standards, I suggest that recovery plans be explicitly guided by and organized around a population viability modeling framework even if data or agency resources are too limited to complete a viability model. When data and resources are available, recovery criteria can be developed from the population viability model results, but when data and resources are insufficient for model implementation, extinction risk thresholds can be used as criteria. A recovery‐planning approach centered on viability modeling will also yield appropriately focused data‐acquisition and monitoring plans and will facilitate a seamless transition from recovery planning to delisting.Un Marco de Referencia para Desarrollar Criterios de Recuperación Objetivos y Medibles para Especies Amenazadas y en Peligro
Long‐Term Doppler Hemodynamics and Effective Orifice Areas of Edwards SAPIEN and Medtronic CoreValve Prostheses after TAVI
Authors: Spethmann Sebastian , Dreger Henryk , Baldenhofer Gerd , Pflug Eyleen , Sanad Wasiem , Stangl Verena , Baumann Gert , Grubitzsch Herko , Sander Michael , Stangl Karl , Laule Michael , Knebel Fabian 
Published: October 2013

BackgroundAlthough initial hemodynamics of percutaneously implanted aortic bioprostheses compare favorably to surgically implanted valves, the durability of the flow characteristics remains unknown. As biological prostheses are at potential risk for early degeneration, the aim of our study was to compare Doppler hemodynamics and effective orifice area (EOA) directly after and at least 1 year after valve implantation.MethodsIn this monocentric, prospective study, we determined peak velocity, peak and mean systolic gradients, and EOA by echocardiography in 75 patients (Edwards SAPIEN, n = 20; CoreValve, n = 55) 1 week (median 7 ± 25 days) and 1 year (median 378 ± 157 days, maximum 1034 days) after transcatheter aortic valve implantation (TAVI).ResultsAfter 12 months, Doppler performance of the aortic valve prostheses remained unchanged. The peak instantaneous velocity was 1.9 ± 0.4 m/s directly after TAVI versus 1.8 ± 0.5 m/s (P = ns) at follow‐up, with a mean gradient of 8.5 ± 3.7 mmHg and 8.1 ± 4.2 (P = ns), respectively. Interestingly, the degree of mitral regurgitation (MR) decreased significantly (P = 0.007) over time, and the severity of aortic regurgitation (AR) remained unchanged during follow‐up (P = ns).ConclusionFor at least 1 year after TAVI, the excellent Doppler hemodynamics and EOA are preserved in transcatheter aortic valve prostheses, and the severity of MR decreased significantly. In addition, we found no evidence of early valve deterioration or progression of AR.
Delayed orthodontic extrusion of a traumatically intruded immature upper permanent incisor – a case report
Authors: Umesan Uday Kumar , Chua Kui Lay , Kok Ei Chuen 
Published: October 2013

AbstractDental injuries are probably the most common orofacial injuries sustained during sports and play. Intrusion or intrusive luxations occur when the teeth are driven into the alveolus by an axially directed impact. This article describes the clinical case of an 8‐year‐old patient who suffered complete intrusive luxation of an immature permanent central incisor following an accident. An attempt at spontaneous re‐eruption failed to facilitate re‐emergence of the tooth beyond 2 mm. The recommended wait‐and‐see strategy in anticipation of spontaneous re‐eruption should cease to be an option when no further elongation can be observed. Orthodontic extrusion or surgical re‐positioning is a valid treatment alternative when the former fails. In this case, although the recommended time period for effecting orthodontic extrusion had elapsed, this procedure was undertaken successfully presumably owing to the application of controlled, biologically optimal forces. A review of the extruded tooth a year later revealed no clinical or radiographic signs of loss of vitality. This was confirmed radiographically by the completion of root development, an intact lamina dura and no evident signs of resorption.
Bumble bee pollen use and preference across spatial scales in human‐altered landscapes
Authors: JHA SHALENE , STEFANOVICH LEV , KREMEN CLAIRE 
Published: October 2013

While the discussion of native pollinator decline has grown dramatically worldwide, information on how native pollinators utilise floral resources in natural and human‐altered landscapes remains relatively limited. Specifically, little is known about the collection of pollen, an essential component of larval and adult bee food, and whether pollen collection patterns change across habitats, spatial scales, and/or native and non‐native floral resource distributions.
In this study, the pollen collection patterns and preferences of the yellow‐faced bumble bee, Bombus vosnesenskii, were examined across a gradient of natural and human‐altered sites.
Three hypotheses were investigated: (i) landscape‐scale (2 km) but not local‐scale (250 m) floral and habitat composition factors are most predictive of bee‐collected pollen composition; (ii) collection of native pollen is greater in sites with greater proportions of natural habitat at landscape scales; and (iii) bees exhibit greater ‘preference’ for native versus non‐native pollen.
Bombus vosnesenskii collected more species of pollen when landscape‐scale riparian forest cover was low but local‐ and landscape‐scale floral species richness was high. Also, B. vosnesenskii collected pollen from a wide range of plant families and did not exhibit a significant preference for native versus non‐native species.
Finally, preference analysis indicated that the only significant preference exhibited by B. vosnesenskii during the study period was for Heteromeles arbutifolia, a native shrub. Overall, results from this study reveal the importance of species‐rich floral patches (native and non‐native) for pollinator provisioning across natural and human‐altered landscapes.
Typology of historical sources and the reconstruction of long‐term historical changes of riverine fish: a case study of the Austrian Danube and northern Russian rivers
Authors: Haidvogl Gertrud , Lajus Dmitry , Pont Didier , Schmid Martin , Jungwirth Mathias , Lajus Julia 
Published: October 2013

AbstractHistorical data are widely used in river ecology to define reference conditions or to investigate the evolution of aquatic systems. Most studies rely on printed documents from the 19th century, thus missing pre‐industrial states and human impacts. This article discusses historical sources that can be used to reconstruct the development of riverine fish communities from the Late Middle Ages until the mid‐20th century. Based on the studies of the Austrian Danube and northern Russian rivers, we propose a classification scheme of printed and archival sources and describe their fish ecological contents. Five types of sources were identified using the origin of sources as the first criterion: (i) early scientific surveys, (ii) fishery sources, (iii) fish trading sources, (iv) fish consumption sources and (v) cultural representations of fish. Except for early scientific surveys, all these sources were produced within economic and administrative contexts. They did not aim to report about historical fish communities, but do contain information about commercial fish and their exploitation. All historical data need further analysis for a fish ecological interpretation. Three case studies from the investigated Austrian and Russian rivers demonstrate the use of different source types and underline the necessity for a combination of different sources and a methodology combining different disciplinary approaches. Using a large variety of historical sources to reconstruct the development of past fish ecological conditions can support future river management by going beyond the usual approach of static historical reference conditions.
Suitability of biochars (pyro‐ and hydrochars) for metal immobilization on former sewage‐field soils
Authors: Wagner A. , Kaupenjohann M. 
Published: October 2013

SummaryMineralization of organic material and decreasing pH are expected to increase bioavailability and leaching of metals in soils formerly contaminated by irrigation with wastewater. Biochar has recently been proposed as a sorbent for metals, although the effects described in the literature are still inconsistent. Therefore, we conducted pot experiments to study the effects of the application of two different biochars to a sewage‐field soil on growth and nutrition of oat (Avena sativa L.) as well as on the mobility of zinc (Zn), copper (Cu), cadmium (Cd) and lead (Pb) in the soil solution. Poplar‐derived hydrochar (obtained by hydrothermal carbonization) and maize‐derived pyrochar (obtained by pyrolysis) were mixed with the soil at concentrations of 0, 1, 2.5 and 5% (g 100 g−1). Soils were leached after 2, 3 and 5 weeks with deionized water. Hydrochar reduced biomass production and had almost no effect on metal concentrations in plants and leachates. The negative effect on biomass yield may be attributable to decreased nitrogen (N) availability. Pyrochar increased biomass production and reduced plant Zn and Cd concentrations. In contrast, metal concentrations in soil leachates generally increased. Comparison of ultra‐centrifuged and filtered samples, microprobe analysis of centrifugation residues and Visual Minteq calculations suggest colloidal transport of Zn precipitates, whereas Cu is transported in the dissolved fraction. We conclude that the tested hydrochar is not suitable for metal immobilization. In the case of pyrochar, further research on colloidal transport of metal precipitates is needed to assess its suitability for soil remediation, despite positive effects on plant growth and the reduced uptake of Zn and Cd.
Appliance‐induced osteopenia of dentoalveolar bone in the rat: effect of reduced bone strains on serum bone markers and the multifunctional hormone leptin
Authors: Vinoth Jayaseelan K. , Patel Kaval J. , Lih Wei‐Song , Seow Yian‐San , Cao Tong , Meikle Murray C. 
Published: October 2013

To understand, in greater detail, the molecular mechanisms regulating the complex relationship between mechanical strain and alveolar bone metabolism during orthodontic treatment, passive cross‐arch palatal springs were bonded to the maxillary molars of 6‐wk‐old rats, which were killed after 4 and 8 d. Outcome measures included serum assays for markers of bone formation and resorption and for the multifunctional hormone leptin, and histomorphometry of the inter‐radicular bone. The concentration of the bone‐formation marker alkaline phosphatase (ALP) was significantly reduced at both time points in the appliance group, accompanied by a 50% reduction in inter‐radicular bone volume; however, osteocalcin (bone Gla protein) levels remained unaffected. Bone collagen deoxypyridinoline (DPD) crosslinks increased 2.3‐fold at 4 d only, indicating a transient increase in bone resorption; in contrast, the level of the osteoclast‐specific marker, tartrate‐resistant acid phosphatase 5b (TRACP 5b), was unchanged. Leptin levels closely paralleled ALP reductions at both time points, suggesting an important role in the mechanostat negative‐feedback loop required to normalize bone mass. These data suggest that an orthodontic appliance, in addition to remodeling the periodontal ligament (PDL)–bone interface, may exert unexpected side‐effects on the tooth‐supporting alveolar bone, and highlights the importance of recognizing that bone strains can have negative, as well as positive, effects on bone mass.
Persistence to Unrewarding Feeding Locations by Honeybee Foragers (Apis mellifera): the Effects of Experience, Resource Profitability and Season
Authors: Al Toufailia Hasan 
Published: October 2013

AbstractHoneybee foragers that find a profitable food source quickly establish spatiotemporal memories, which allow them to return to this foraging site on subsequent days. The aim of this study was to investigate how the previous experience of honeybee foragers at a feeding location affects their persistence at that location once food is no longer available. We hypothesised that persistence would be greater to locations that were more rewarding (closer to the hive, higher concentration of sucrose solution), for which a bee had greater prior experience (0.5‐h vs. 2‐h training access), and at times of the year of lower nectar availability in the environment. We studied individually marked worker bees from four colonies trained to sucrose‐solution feeders. Our results support most of these predictions. Persistence, measured both in duration and number of visits, was greater to locations that previously offered sucrose solution of higher concentration (2 m vs. 1 m) or were closer to the hive (20 m vs. 450 m). Persistence was also greater in bees that had longer access at the feeder before the syrup was terminated (2 h vs. 0.5 h). However, contrary to our prediction, persistence was not higher in the season of the lowest nectar availability in the environment in the study year. In summary, honeybees show considerable persistence at foraging sites that ceased providing rewards. The decision to abandon a foraging site depends on the profitability the forager experienced when the foraging site was still rewarding.
Commentary on: Hairless and the polyamine putrescine form a negative regulatory loop in the epidermis
Authors: Ramot Yuval , Vardy Leah A. 
Published: October 2013

AbstractPolyamines are cationic amines essential for cellular proliferation. Recently, their role in hair follicle (HF) growth has started to be explored, but their exact function is still obscure. In the October issue of Experimental Dermatology, Luke et al. follow the observation that putrescine overproducing mice and hairless (HR) mutant mice show a similar clinical phenotype of hair loss and dermal cyst formation. They show that HR and putrescine form a negative regulatory feedback mechanism, which might regulate hair cycling and therefore control hair growth. This study clearly demonstrates that a strong connection exists between HR and polyamines although there are probably additional molecular pathways involved in the polyamine regulation of hair growth which remain to be discovered.
No human virus sequences detected by next‐generation sequencing in benign verrucous skin tumors occurring in BRAF‐inhibitor‐treated patients
Authors: Ganzenmueller Tina , Hage Elias , Yakushko Yuri , Kluba Jeanette , Woltemate Sabrina , Schacht Vivien , Schulz Thomas F. , Gutzmer Ralf 
Published: October 2013

AbstractPatients treated with BRAF inhibitors (e.g. vemurafenib), a novel targeted therapy for advanced melanoma harbouring certain BRAF mutations, develop numerous adverse cutaneous side effects, including skin tumors such as squamous cell carcinoma or non‐malignant verruciform keratinocyte proliferations, termed ‘BRAF‐inhibitor‐associated verrucous keratosis (BAVK) lesions’. These keratinocyte proliferations are believed to be caused by paradoxical hyperactivation of the MAPK pathway in cells with wild‐type BRAF, but mutated RAS. However, due to the clinical and histological verruca‐like appearance of these lesions, additional aetiologic cofactors, such as infectious agents (i.e. oncogenic viruses), might be suspected. Therefore, we performed 454 high‐throughput sequencing of BAVK lesions from vemurafenib‐treated patients on the transcript level to identify actively transcribed viral sequences of known [e.g. human papilloma viruses (HPV)] or even yet‐unknown viruses. Next‐generation sequencing did not identify transcripts of any human viruses out of 1 595 161 reads obtained from BAVK lesions of four patients. Nevertheless, all controls were recognized correctly, and the detection of sequences derived from the cutaneous microbiome (e.g. skin commensals and bacterial phages) confirmed the validity and sensitivity of the sequencing data. Our results are consistent with preliminary histological and immunohistochemical findings recently reported by others, who also failed to detect the expression of HPV proteins in BAVK. Although the patient number is limited and we cannot exclude the possibility of having missed a viral transcript of very low abundance, our study argues against a viral aetiology of BRAF‐inhibitor‐associated verruciform keratoses occurring under vemurafenib.
Simvastatin induces the osteogenic differentiation of human periodontal ligament stem cells
Authors: Zhao Bing‐jiao , Liu Yue‐hua 
Published: October 2013

AbstractPeriodontal ligament stem cells (PDLSCs) are considered as potential mesenchymal stem cell sources for future clinical applications in periodontal regeneration therapy. Simvastation, widely used for lowering serum cholesterol, is known to have a bone stimulatory effect. However, it is not clear whether simvastation affects the differentiation of PDLSCs. This study examined the effects of simvastatin on human PDLSCs in vitro and in vivo. Using the limiting dilution technique, human PDLSCs were isolated and expanded. PDLSCs were cultured with simvastatin (0.01–10 μm), and the proliferation was measured. The osteogenic differentiation was characterized by alkaline phosphatase (ALP) activity and Alizarin Red‐S staining for calcium deposition. The gene expression levels of osteogenic markers were evaluated by RT‐PCR. In addition, PDLSCs were transplanted into nude mice with ceramic bovine bone powders as carriers to observe the capacity of mineralized tissue formation in vivo. Simvastatin at concentrations <1 μm did not suppress the proliferation of PDLSCs. After the administration of 0.1 μm simvastatin, the expression of ALP, bone sialoprotein, and bone morphogenetic protein‐2 genes were significantly upregulated, and the ALP activity and mineralized nodule formation were significantly higher in the simvastatin‐treated cells than the control cells. In addition, the in vivo transplantation results showed that simvastatin treatment promoted the degree of mineralized tissue formation. Collectively, simvastatin has positive effects on osteogenic differentiation of human PDLSCs in vitro and in vivo. This suggests that simvastatin might be a useful osteogenic induction agent for periodontal bone regeneration.