bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment for acceleration and transformation of chronic lymphocytic leukemia/small lymphocytic lymphoma using histologic and immunohistochemical features: a case series
Authors: Moore Margaret E. , Aguilera Nadine S. , Obiorah Ifeyinwa , Williams Eli , Courville Elizabeth 
Published: July 2024

AbstractMorphologic features of aggressive/ “accelerated” chronic lymphocytic leukemia/small lymphocytic lymphoma (aCLL/SLL) have been described. Richter transformation (RT) also occurs in a subset of CLL/SLL cases. This case series examined inter-observer variability when assessing for aCLL/SLL and RT, with attention to how immunohistochemical (IHC) markers may assist in this evaluation. Twelve cases of CLL/SLL with available FFPE tissue were identified. H&E staining and IHC (CD3, CD20, CD5, CD23, LEF1, LAG3, C-MYC, PD-1, MUM1, Cyclin D1, BCL-6, p53, and Ki-67) were performed. Three hematopathologists reviewed each case. The pathologists provided a final interpretation of (1) CLL/SLL, (2) CLL/SLL with expanded and/or confluent proliferation centers or increased Ki-67 (aCLL/SLL), or (3) large cell transformation/DLBCL. The pathologists lacked consensus in the diagnosis in 6/12 cases (50%). The reviewers disagreed on the presence of expanded/confluent proliferation centers in 8/12 cases (67%). With the exception of Ki-67, no IHC marker showed a difference in the staining profile in aCLL/SLL or RT compared to low-grade cases. This series showed inter-observer variability in the evaluation for aCLL/SLL and RT. A study that serially examines genetic alterations in FFPE tissue and correlates the features with histology and IHC, at diagnosis and throughout the disease course, may help refine indicators of aggressive disease.
A retrospective study of the efficacy of sulbactam in the treatment of patients with extensively drug-resistant Acinetobacter baumannii infections
Authors: Yu Jiaxin , Zhang Baoshuang , Yang Yang , Dou Wei , Li Yuliu , Yang Anji , Ruan Xiao , Zuo Wei , Zhang Bo 
Published: July 2024

Abstract
Purpose
Sulbactam (SBT) is one of the most significant treatments for patients with extensively drug-resistant Acinetobacter baumannii (XDR-AB). However, the efficacy and safety of SBT and its high dose regimen has not been well documented. This retrospective study aimed to assess the efficacy and safety of SBT-based treatment, particularly at high-dose (≥ 6 g/day), for XDR-AB infection.

Method
A total of 52 XDR-AB infected patients treated with intravenous SBT at Peking Union Medical College Hospital were included. The primary outcome was 28-day all-cause mortality, while the secondary outcome was 14-day clinical response and the time of response. The formulation of SBT in our study is 0.5 g per vial.

Results
Among the patients, the 28-day all-cause mortality rate was 36.5% (19/52), and the favorable 14-day clinical response rate was 59.6% (31/52). The 28-day mortality was independently associated coinfection with gram-positive bacteria (GPB) and a shorter duration of therapy. Patients with intracranial infection might have a longer survival time. A favorable 14-day clinical response was associated with the dose of SBT, and a longer treatment duration. However, the higher creatinine clearance (CrCl) associated with a worse clincal response. In addition, a higher SBT dosage was significantly correlated with a shorter time to clinical response. No adverse effects related were reported.

Conclusion
The single-agent formulation of SBT emerges as a promising alternative for the treatment of XDR-AB infection, such as intracranial infection, particularly at high doses (≥ 6 g/day). Besides, longer duration of treatment correlates with higher survival rate and better favorable clinical response. Higher CrCl negatively correlates with favorable clinical response.
From perturbative methods to machine learning techniques in space science
Authors: Celletti Alessandra 
Published: July 2024

AbstractPerturbation theory is a very useful tool to investigate the dynamics of models in space science. We start by presenting some results obtained implementing classical perturbation theory to investigate the motion of space debris, which are objects that populate the sky around the Earth after a satellite break-up event. When dealing with two or more break-up events, a clusterization of the fragments can be computed using machine learning techniques. We also present the celebrated KAM theory for symplectic and conformally symplectic systems. We recall several computer-assisted results in Celestial Mechanics in conservative and dissipative settings. Finally, we consider the spin-orbit problem and we show how machine learning methods can be conveniently used to classify regular and chaotic motions.
Introducing the Research Design of Phenomenography
Authors: Sims Danica Anne 
Published: July 2024

AbstractThis article introduces the lesser known qualitative research design of phenomenography to medical science and health professions education researchers. Phenomenography, as distinct from phenomenology, seeks to describe and organise the different ways people experience and understand a phenomenon. Here, the origins and philosophical underpinnings of phenomenography are briefly shared, and an outline of how a phenomenographic research study may be undertaken is presented. This includes data collection tools, analytic method, and examples from the field. Overall, phenomenography is valuable for better understanding the varied experiences of students, educators, practitioners, and patients, with implications for pedagogy, practice, and related outcomes.
Shadows in the Spotlight: Navigating Organizational Transparency in Digital Contexts
Authors: Albu Oana Brindusa , Thøger Christensen Lars 
Published: July 2024

Abstract
This article examines the potential implications of pursuing organizational transparency ideals in contemporary datafied societies, i.e., societies where multiple aspects of social life take the shape of digital data. The article develops a communication perspective of transparency which is valuable because it not only considers transparency as a matter of providing and accessing information about already existing practices but conceptualizes transparency as a performative practice with the potential to shape subjects, relations, and forms of organizing. The article identifies common trends in extant research on transparency, discusses different ways of thinking about transparency, and examines their implications for organizational settings. Taken together, the article problematizes predominant tendencies that theorize transparency simply from an informational standpoint, (i.e., as information disclosure) and identifies key challenges specific to digitally driven transparency. The article ends by discussing some implications of digitally driven transparency practices and sketches future research directions for organizations that face transparency challenges in the current social contexts where data is abundant.
Subchronic administration of scopolamine reverses UCMS-induced behavior in mice via eEF2 protein dephosphorylation
Authors: Babii Yana , Pałucha-Poniewiera Agnieszka , Rafało-Ulińska Anna , Brański Piotr , Pilc Andrzej 
Published: July 2024

Abstract
Background
The cholinergic system has been increasingly linked to the pathophysiology of mood disorders such as depression, with the potential involvement of nicotinic and/or muscarinic receptors. Conventional antidepressants usually require weeks of daily dosing to achieve a full antidepressant response. In contrast, clinical studies have shown that scopolamine, a nonselective muscarinic acetylcholine receptor antagonist, can induce potent and rapid antidepressant effects, requiring only a few days of treatment. This study aimed to examine the suitability of the unpredictable chronic mild stress (UCMS) model of depression to reproduce the above scopolamine antidepressant activity patterns.

Methods
Rapid and sustained antidepressant-like effects were assessed by using the splash test, sucrose preference test (SPT), tail suspension test (TST), and forced swimming test (FST) in animals undergoing the UCMS procedure and stress-naïve C57BL/6J mice. Western Blotting was used to measure tropomyosin receptor kinase B (TrkB), mammalian target of rapamycin (mTOR), eukaryotic elongation factor (eEF2) and postsynaptic density protein 95 (PSD95) levels.

Results
Scopolamine induced antidepressant-like effects in a dose-dependent manner only after subchronic, but not single, administration in the UCMS model of depression in C57BL/6J mice without affecting locomotor activity. Specifically, scopolamine administered at a dose of 0.3 mg/kg for four consecutive days significantly reversed the UCMS-induced depressive-like behavior, such as apathy, anhedonia, and behavioral despair, while scopolamine, given at the same dose but only once, did not relieve the above symptoms. Scopolamine treatment was accompanied by eEF2 protein dephosphorylation and its subsequent reactivation in the prefrontal cortex (PFC).

Conclusion
Subchronic administration of scopolamine is needed to ameliorate UCMS-induced depressive-like behavior. The suggested mechanism of scopolamine action covers eEF2 protein activity in the PFC.
Lagrangian Decomposition of the Atlantic Ocean Heat Transport at 26.5°N
Authors: Tooth Oliver J. , Foukal Nicholas P. , Johns William E. , Johnson Helen L. , Wilson Chris 
Published: July 2024

AbstractThe Atlantic Meridional Overturning Circulation (AMOC) plays a critical role in the global climate system through the redistribution of heat, freshwater and carbon. At 26.5°N, the meridional heat transport has traditionally been partitioned geometrically into vertical and horizontal circulation cells; however, attributing these components to the AMOC and Subtropical Gyre (STG) flow structures remains widely debated. Using water parcel trajectories evaluated within an eddy‐rich ocean hindcast, we present the first Lagrangian decomposition of the meridional heat transport at 26.5°N. We find that water parcels recirculating within the STG account for 37% (0.36 PW) of the total heat transport across 26.5°N, more than twice that of the classical horizontal gyre component (15%). Our findings indicate that STG heat transport cannot be meaningfully distinguished from that of the basin‐scale overturning since water parcels cooled within the gyre subsequently feed the northward, subsurface limb of the AMOC.
Spatial Source Contribution and Interannual Variation in Deposition of Dust Aerosols Over the Chinese Loess Plateau
Authors: Haugvaldstad Ove W. , Tang Hui , Kaakinen Anu , Bohm Katja , Groot Zwaaftink Christine D. , Grythe Henrik , Stevens Thomas , Zhang Zhongshi , Stordal Frode 
Published: July 2024

AbstractThe Chinese Loess Plateau (CLP) in northern China is home to one of the most prominent loess records in the world, reflecting past eolian dust activity in East Asia. However, their interpretation is hampered by ambiguity in the origin of loess‐forming dust and an incomplete understanding of the circulation forcing dust accumulation. In this study, we used a novel modeling approach combining a dust emission model FLEXDUST with simulated back trajectories from FLEXPART to trace the dust back to where it was emitted. Over 21 years (1999–2019), we modeled back trajectories for fine (∼2 μm) and super‐coarse (∼20 μm) dust particles at six CLP sites during the peak dust storm season from March to May. FLEXPART source‐receptor relationships are combined with the dust emission inventory from FLEXDUST to create site‐dependent high‐resolution maps of the source contribution of deposited dust. The nearby dust emission areas were found to be the main source of dust to the CLP. Dust deposition across the CLP was found to predominantly occur via wet removal, with also some super‐coarse dust from distant emission regions being wet deposited following high‐level tropospheric transport. The high topography located on the downwind side of the emission area plays an essential role in forcing the emitted super‐coarse dust upward. On an interannual scale, the phase of the Arctic Oscillation in the preceding winter was found to have a strong association with the spring deposition rate on the CLP, while the strength of the East Asian Winter Monsoon was less influential.
Stable Water Isotope Signals and Their Relation to Stratiform and Convective Precipitation in the Tropical Andes
Authors: Landshuter Nadja , Aemisegger Franziska , Mölg Thomas 
Published: July 2024

AbstractStratiform and convective precipitation are known to be associated with distinct isotopic fingerprints in the tropics. Such rain type specific isotope signals are of key importance for climate reconstructions derived from climate proxies (e.g., stable isotopes in tree rings). Recently, the relation between rain type and isotope signal in present‐day climate has been intensively discussed. While some studies point out the importance of deep convection, other studies emphasize the role of stratiform precipitation for strongly depleted isotope signals in precipitation. Uncertainties arise from observational studies due to data scarcity while modeling approaches with global climate models cannot explicitly resolve convective processes and rely on parameterizations. High‐resolution climate models are particularly important for studies over complex topography and for the simulation of convective cloud formation and organization. Therefore, we applied the isotope‐enabled version of the high‐resolution climate model from the Consortium for Small‐Scale Modeling (COSMOiso) over the Andes of tropical south Ecuador, South America, to investigate the influence of stratiform and convective rain on the stable oxygen isotope signal of precipitation (δ18OP). Our results highlight the importance of deep convection for depleting the isotopic signal of precipitation and increasing its deuterium excess. Due to the opposing effect of shallow and deep convection on the δ18OP signal, the use of a stratiform fraction might be misleading. We therefore propose to use a shallow and deep convective fraction to analyze the effect of rain types on δ18OP.
A Semi‐Mechanistic Model for Partitioning Evapotranspiration Reveals Transpiration Dominates the Water Flux in Drylands
Authors: Reich E. G. , Samuels‐Crow K. , Bradford J. B. , Litvak M. , Schlaepfer D. R. , Ogle K. 
Published: July 2024

AbstractPopular evapotranspiration (ET) partitioning methods make assumptions that might not be well‐suited to dryland ecosystems, such as high sensitivity of plant water‐use efficiency (WUE) to vapor pressure deficit (VPD). Our objectives were to (a) create an ET partitioning model that can produce fine‐scale estimates of transpiration (T) in drylands, and (b) use this approach to evaluate how climate controls T and WUE across ecosystem types and timescales along a dryland aridity gradient. We developed a novel, semi‐mechanistic ET partitioning method using a Bayesian approach that constrains abiotic evaporation using process‐based models, and loosely constrains time‐varying WUE within an autoregressive framework. We used this method to estimate daily T and weekly WUE across seven dryland ecosystem types and found that T dominates ET across the aridity gradient. Then, we applied cross‐wavelet coherence analysis to evaluate the temporal coherence between focal response variables (WUE and T/ET) and environmental variables. At yearly scales, we found that WUE at less arid, higher elevation sites was primarily limited by atmospheric moisture demand, and WUE at more arid, lower elevation sites was primarily limited by moisture supply. At sub‐yearly timescales, WUE and VPD were sporadically correlated. Hence, ecosystem‐scale dryland WUE is not always sensitive to changes in VPD at short timescales, despite this being a common assumption in many ET partitioning models. This new ET partitioning method can be used in dryland ecosystems to better understand how climate influences physically and biologically driven water fluxes.
Solar‐Induced Chlorophyll Fluorescence as a Potential Proxy for Gross Primary Production and Methane Emission in a Cool‐Temperate Bog in Northern Japan
Authors: Buareal Kanokrat , Kato Tomomichi , Morozumi Tomoki , Nakashima Naohisa , Tanatarakeree Kitpanuwat , Ueyama Masahito , Hirano Takashi 
Published: July 2024

AbstractWetlands play an essential role in the global greenhouse gas budget via carbon dioxide sequestration as well as methane emission. In recent decades, solar‐induced chlorophyll fluorescence (SIF) has been recognized as a remotely sensed proxy of gross primary productivity (GPP), which generates substrates for methane production. To examine the suitability of SIF for estimation of these two fluxes, we conducted ground tower‐based SIF observation with an ultrafine‐resolution spectroradiometer in conjunction with eddy covariance measurement in a cool‐temperate bog. The daily SIF retrieved in the red (687 nm) and far‐red (760 nm) bands (SIFred and SIFfar‐red, respectively) increased nonlinearly with GPP and linearly with absorbed photosynthetically active radiation (APAR). The relatively weak correlation between apparent SIF yield (ΦSIF = SIF/APAR) and light use efficiency implied that both APAR and plant physiology constrained the SIF emission in this wetland. The SIFred/SIFfar‐red ratio showed a significant negative relationship with vegetation greenness indices, and the similar seasonal variation in SIFred and SIFfar‐red indicated that the SIFred reabsorption effect only weakly influenced the SIFred–GPP relationship. Episodic temporal reduction in the water table did not distinctly influence SIF and ΦSIF. Estimation of the methane emission rate was subtly improved by incorporating SIF, which was substituted for GPP as the methanogenesis substrate, in a multivariable regression analysis together with two environmental factors: soil temperature and water table depth. This study illustrates the potential of both SIFred and SIFfar‐red to monitor GPP and to predict methane emission in wetlands.
Seasonal and Fortnight Variations in Internal Solitary Waves in the Indonesian Seas From the SWOT Measurements
Authors: Qiu Bo , Chen Shuiming , Wang Jinbo , Fu Lee‐Lueng 
Published: July 2024

AbstractUsing the wide‐swath sea surface height (SSH) data from the 1‐day repeat calibration/validation phase of the Surface Water and Ocean Topography (SWOT) mission, we explored fine‐scale evolution of nonlinear internal solitary waves (ISWs) in the Banda/Molucca Seas in the Indonesian Archipelagos. Generated in the Ombai Strait and Lifamatola Passage through semidiurnal tide‐topography interaction, respectively, ISWs in the Banda/Molucca Seas have a SSH amplitude of 10–20 cm and exhibit multiple wave packets with the leading wave crest followed by a series of rank‐ordered secondary wave crests. Due to nonlinearity, the ISWs are observed to propagate northward at a speed faster than the regional mode‐1 internal gravity waves and their amplitudes are modulated fortnightly following the regional spring‐neap tidal cycle. On longer timescale, the observed ISW amplitudes are controlled by seasonal upper ocean stratification changes with a weaker stratification favoring a larger amplitude. By converting the SWOT‐measured SSH data to the interior ocean pressure signals, we quantified the depth‐integrated energy flux associated with the northward‐propagating ISWs to be 5 and 2 kW m−1 in the central Banda and Molucca Seas, respectively. Comparisons with past studies indicate that the ISWs contribute to close to 100% and 40% of the tidally‐induced, northward energy fluxes, respectively, across the Banda and Molucca Seas.
Detection of congenital heart disease by neonatologist performed cardiac ultrasound in preterm infants
Authors: MacDonald Bradley , Yim Deane , Ramsay James , Gill Andrew 
Published: July 2024

Abstract
Objective
We aimed to assess the frequency of de novo congenital heart disease (CHD) detection via neonatologist-performed cardiac ultrasounds (NPCU) in premature infants born at <30 weeks of gestation.

Study design
In this cross-sectional study (2004–2023) clinicians completing NPCU flagged de novo suspected CHD. All flagged NPCUs were cross-checked with cardiologists to confirm CHD diagnosis.

Results
There were 2088 out of 3739 infants (56%) with at least one NPCU; 294 (14%) with cardiology referral. CHD diagnosis was confirmed in 109 of the 2088 (5.2%) infants. All major and critical CHD on NPCU imaging were suspected during NPCU and had prompt referral to the cardiology department.

Conclusion
De novo presentation of significant CHD continues to occur in the preterm population, emphasizing the need for recognizing CHD during NPCU. Optimizing NPCU training may benefit patients with early cardiology referral and review.
In vivo CRISPR/Cas9-mediated screen reveals a critical function of TFDP1 and E2F4 transcription factors in hematopoiesis
Authors: Tran Ngoc Tung , Graf Robin , Acevedo-Ochoa Ernesto , Trombke Janine , Weber Timm , Sommermann Thomas , Salomon Claudia , Kühn Ralf , Rajewsky Klaus , Chu Van Trung 
Published: July 2024

AbstractHematopoiesis is a continuous process of blood cell production driven by hematopoietic stem and progenitor cells (HSPCs) in the bone marrow. Proliferation and differentiation of HSPCs are regulated by complex transcriptional networks. In order to identify transcription factors with key roles in HSPC-mediated hematopoietic reconstitution, we developed an efficient and robust CRISPR/Cas9-based in vivo genetic screen. Using this experimental system, we identified the TFDP1 transcription factor to be essential for HSPC proliferation and post-transplant hematopoiesis. We further discovered that E2F4, an E2F transcription factor, serves as a binding partner of TFDP1 and is required for HSPC proliferation. Deletion of TFDP1 caused downregulation of genes associated with the cell cycle, with around 50% of these genes being identified as direct targets of TFDP1 and E2F4. Thus, our study expands the transcriptional network governing hematopoietic development through an in vivo CRISPR/Cas9-based genetic screen and identifies TFDP1/E2F4 as positive regulators of cell cycle genes in HSPCs.
Adherence to Mediterranean dietary pattern and the risk of gestational diabetes mellitus: a systematic review and meta-analysis of observational studies
Authors: Jafari Nasab Saeede , Ghanavati Matin , C. T.Clark Cain , Nasirian Maryam 
Published: July 2024

Abstract
Background and aim
Gestational diabetes mellitus (GDM) is one of the most prevalent disorders occurring during pregnancy, which confers significant risk of short and long-term adverse outcomes in both mothers and offspring. Recently, more attention has been paid to the association of pre-pregnancy and early pregnancy healthy dietary patterns, such as Mediterranean dietary pattern with GDM. However, there is a lack of systematic review and meta-analysis summarizing findings in this regard. Hence, we sought to assess the association of MedDiet and GDM in observational studies by performing a systematic review and meta-analysis.

Methods
A comprehensive systematic literature search of observational studies was conducted via PubMed, Scopus, and Google Scholar, up to August 2023. Studies were included in our review if they evaluated the association of MedDiet and GDM, following an observational study design.

Results
Ten studies were included in this study. Combining effect sizes, we found that adherence to MedDiet was inversely associated with GDM risk (OR = 0.64; CI: 0.52–0.78); implying that higher adherence to the MedDiet could reduce the risk of GDM by about 36%. Stratification by the geographic area, Mediterranean countries, time of dietary assessment and study design, showed a consistent significant association between MedDiet and GDM.

Conclusion
We conclude that adhering to diets resembling MedDiet, before or in early pregnancy, could be associated with lower risks or odds of GDM.