bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thieno–Pyrrole‐Fused BODIPY Intermediate as a Platform to Multifunctional NIR Agents
Authors: Awuah Samuel G. , Das Sushanta K. , D'Souza Francis , You Youngjae 
Published: August 2013

AbstractWe report the synthesis, photophysical and electrochemical properties, and in vivo fluorescence imaging of a series of new thieno–pyrrole‐fused near‐infrared (NIR) BODIPY agents by using a versatile intermediate as a building block. The versatile thieno–pyrrole‐fused BODIPY intermediate was rationally designed to bear bromo‐substituents and absorb in the mid‐red region (635 nm) to act as an organic electrophile for the development of NIR multifunctional agents. The use of subsequent palladium‐catalyzed and nucleophilic substitution reactions afforded highly conjugated NIR BODIPYs. The novel BODIPYs exhibit long‐wavelength absorptions in the NIR region (650–840 nm). The agents produce sharp fluorescence bands, and most of them display respectable quantum yields of fluorescence (0.05–0.87) useful for biomedical imaging, as demonstrated by in vivo imaging with SBDPiR740. Interestingly, a number of agents in the series that are non‐halogenated were reactive to O2 at the triplet photo‐excited state coupled with a favorable redox potential and decent fluorescence, and hence could be potential candidates for use as photosensitizers in fluorescence‐guided photodynamic therapy. Furthermore, the synthetic approach allows further functionalization of the highly conjugated NIR BODIPYs to tune the excited states (PET, ICT) and to conjugate targeting moieties for enhanced biological applications.
Phenotypic modifications in ovarian cancer stem cells following Paclitaxel treatment
Authors: Craveiro Vinicius , Yang‐Hartwich Yang , Holmberg Jennie C. , Sumi Natalia J. , Pizzonia John , Griffin Brian , Gill Sabrina K. , Silasi Dan‐Arin , Azodi Masoud , Rutherford Thomas , Alvero Ayesha B. , Mor Gil 
Published: August 2013

AbstractEpithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy. Despite initial responsiveness, 80% of EOC patients recur and present with chemoresistant and a more aggressive disease. This suggests an underlying biology that results in a modified recurrent disease, which is distinct from the primary tumor. Unfortunately, the management of recurrent EOC is similar to primary disease and does not parallel the molecular changes that may have occurred during the process of rebuilding the tumor. We describe the characterization of unique in vitro and in vivo ovarian cancer models to study the process of recurrence. The in vitro model consists of GFP+/CD44+/MyD88+ EOC stem cells and mCherry+/CD44−/MyD88− EOC cells. The in vivo model consists of mCherry+/CD44+/MyD88+ EOC cells injected intraperitoneally. Animals received four doses of Paclitaxel and response to treatment was monitored by in vivo imaging. Phenotype of primary and recurrent disease was characterized by quantitative polymerase chain reaction (qPCR) and Western blot analysis. Using the in vivo and in vitro models, we confirmed that chemotherapy enriched for CD44+/MyD88+ EOC stem cells. However, we observed that the surviving CD44+/MyD88+ EOC stem cells acquire a more aggressive phenotype characterized by chemoresistance and migratory potential. Our results highlight the mechanisms that may explain the phenotypic heterogeneity of recurrent EOC and emphasize the significant plasticity of ovarian cancer stem cells. The significance of our findings is the possibility of developing new venues to target the surviving CD44+/MyD88+ EOC stem cells as part of maintenance therapy and therefore preventing recurrence and metastasis, which are the main causes of mortality in patients with ovarian cancer.
Borderline trend towards long‐term mortality benefit from drug eluting stents implantation in ST‐elevation myocardial infarction patients in Poland—data from NRDES registry
Authors: Siudak Zbigniew , Dziewierz Artur , Rakowski Tomasz , Żmudka Krzysztof , Legutko Jacek , Bartuś Stanisław , Dragan Jacek , Zasada Wojciech , Tokarek Tomasz , Kułaga Tomasz , Partyka Łukasz , Dudek Dariusz 
Published: August 2013

ObjectivesTo analyze long‐term outcome of ST‐Elevation Myocardial Infarction (STEMI) and non‐STEMI (NSTEMI) patients treated with Drug Eluting Stents (DES) stents with regard to mortality and stent thrombosis rates based on the national Polish PCI registry database.BackgroundOnly a few studies suggested potential trend towards lower mortality in STEMI patients treated with DES. Current European Society of Cardiology STEMI guidelines recommend DES use only with one restriction to suspected patient poor compliance or contraindication to dual antiplatelet therapy.MethodsThirteen high‐volume interventional cardiology centers in Poland enrolled patients in National Registry of Drug Eluting Stents (NRDES) Registry from October 2010 till October 2011.ResultsThere were 2686 patients enrolled in NRDES Registry. Eighty five patients (3%) had both DES and BMS implanted at index PCI procedure and were excluded from further analysis. A subpopulation of STEMI (1709; 66%) and NSTEMI (892; 34%) patients was selected for BMS vs DES comparison. A significant difference in favor of DES group for 1‐year mortality was found in STEMI subgroup (P < 0.0001—unadjusted and P = 0.0497 after propensity score adjustment). No such differences were noticed for NSTEMI subgroup or stent thrombosis comparisons.ConclusionsA strong selection bias for DES stents was observed with regard to demographic and angiographic characteristics in both STEMI and NSTEMI. DES implantation was associated with similar ischemic outcome to BMS at 1‐year follow‐up. STEMI patients with DES presented a trend towards lower long‐term mortality at 1 year in comparison to BMS. © 2013 Wiley Periodicals, Inc.
Long‐term clinical outcomes following drug‐eluting stent implantation for unprotected distal trifurcation left main disease: The Milan‐New Tokyo (MITO) registry
Authors: Ielasi Alfonso , Takagi Kensuke , Latib Azeem , Basavarajaiah Sandeep , Figini Filippo , Carlino Mauro , Montorfano Matteo , Chieffo Alaide , Nakamura Sunao , Colombo Antonio 
Published: August 2013

BackgroundUnprotected distal left main trifurcation (ULMT) lesion represents a challenge for interventional cardiologists with the potential for peri‐procedural complications and adverse events at follow‐up especially when the main branch and the side branches are concomitantly diseased.MethodsA retrospective cohort analysis was performed on consecutive patients with ULMT stenosis who electively underwent percutaneous coronary intervention (PCI) with drug‐eluting stent (DES) implantation in order to assess the technical feasibility and long‐term outcomes according to the disease distribution in the trifurcation branches (true vs. non‐true ULMT). Primary endpoint of the study was a composite of major adverse cardiovascular events (MACE) defined as cardiac‐death, myocardial infarction (MI), and target lesion revascularization (TLR) during follow‐up.ResultsEighty‐four patients underwent PCI with DES for ULMT disease during the study period (40 true trifurcation and 44 non‐true trifurcation). Angiographic and procedural success were obtained in 94% and 92.8% of cases. At 3‐years follow‐up, the occurrence of MACE was significantly higher in patients with true ULMT than in those with non‐true ULMT (HR 2.801 [confidence interval; CI 1.164–7.896], P = 0.025) due to a higher TLR rate (HR 3.032 [CI 1.164–7.896], P = 0.023). No episodes of late and very late definite/probable stent thrombosis (ST) occurred. On multivariable analysis, a true‐ULMT lesion was the only independent predictor of MACE (HR 2.344 [C.I. 1.006–5.461], P = 0.049).ConclusionsPCI with DES for ULMT stenosis is feasible with a high procedural success rate and no definite/probable ST reported at follow‐up. A true trifurcation lesion is associated with enhanced risk of MACE mainly driven by TLR. © 2013 Wiley Periodicals, Inc.
Paravalvular leak closure after transcatheter aortic valve replacement with a self‐expanding prosthesis
Authors: Gafoor Sameer , Franke Jennifer , Piayda Kerstin , Lam Simon , Bertog Stefan , Vaskelyte Laura , Hofmann Ilona , Sievert Horst 
Published: August 2013

IntroductionParavalvular leak (PVL) after percutaneous transcatheter aortic valve replacement (TAVR) is associated with significant morbidity and mortality. Percutaneous PVL closure has been reported for balloon‐expandable valves but not self‐expandable valves.MethodsWe conducted a review of cases at our center where patients who received TAVR with self‐expandable valves and went on to develop severe PVL underwent percutaneous closure. Baseline demographic, TAVR procedural, PVL procedural, and follow‐up data were collected.ResultsA total of five patients with severe PVL after TAVR with a self‐expanding valve underwent percutaneous PVL closure. Four of five patients had a trial of balloon postdilatation after valve deployment and had significant persistent PVL. In all five patients, PVL went from moderate‐severe to mild‐moderate PVL. There were no adverse events.ConclusionPercutaneous PVL closure for severe PVL self‐expanding valve for TAVR is a safe and efficacious procedure. Procedural technique involves transesophageal guidance, a high approach through the valve struts, deployment of an appropriate size device, and careful monitoring. This method may be part of the algorithm for severe PVL after TAVR. © 2013 Wiley Periodicals, Inc.
Photocatalytic Hydrogen Production with Copper Photosensitizer–Titanium Dioxide Composites
Authors: Karnahl Michael , Mejía Esteban , Rockstroh Nils , Tschierlei Stefanie , Luo Shu‐Ping , Grabow Kathleen , Kruth Angela , Brüser Volker , Junge Henrik , Lochbrunner Stefan , Beller Matthias 
Published: August 2013

AbstractA series of heteroleptic copper photosensitizers [($\widehat{PP}$)Cu($\widehat{NN}$(SO3Na)2)]+ containing sulfonate anchor groups is described. In the presence of titanium dioxide they form composites, which are active photosensitizers in the light driven reduction of protons. Further stabilization of these systems is achieved by encapsulation within a plasma‐polymerized allylamine (PPAAm) layer. The resulting PPAAm‐CuPS‐TiO2‐composites exhibit a strong absorption in the visible region of the light. Photocatalytic hydrogen production is performed by using only non‐noble metals. In the presence of an iron reduction catalyst a maximum turnover number of 2452 is obtained.
Controlled Heterocyclization/Cross‐Coupling Domino Reaction of β,γ‐Allendiols and α‐Allenic Esters: Method and Mechanistic Insight for the Preparation of Functionalized Buta‐1,3‐dienyl Dihydropyrans
Authors: Alcaide Benito , Almendros Pedro , Martínez del Campo Teresa , Quirós M. Teresa , Soriano Elena , Marco‐Contelles José L. 
Published: August 2013

AbstractStarting from β,γ‐allendiols and α‐allenic acetates, a chemo‐ and regiocontrolled palladium‐catalyzed methodology has provided access to enantiopure 3,6‐dihydropyrans that bear a buta‐1,3‐dienyl moiety. Thus, it has been demonstrated for the first time that the preparation of six‐membered heterocycles through cross‐coupling reactions of two different allenes can be accomplished. These heterocyclization/cross‐coupling reactions have been developed experimentally and their mechanisms have additionally been investigated by a computational study.
Estimating the Adoption of Transcatheter Aortic Valve Replacement By US Interventional Cardiologists and Clinical Trialists
Authors: Stolker Joshua M. , Patel Akshar Y. , Lim Michael J. , Hauptman Paul J. 
Published: August 2013

AbstractBackgroundDespite extensive attention dedicated to transcatheter aortic valve replacement (TAVR) in both the medical literature and lay press, little is known about the anticipated utilization of TAVR by the US cardiology community.HypothesisTAVR use is likely to outstrip its initial clinical indications.MethodsFour days after approval of the first TAVR device in November 2011 by the US Food and Drug Administration, we emailed an online questionnaire to 201 authors of major TAVR clinical trials (trialists) and 461 recent members of an interventional cardiology professional society (clinicians). Responses were compared using χ2, t tests, and analysis of variance.ResultsOf 205 surveys received (response rate 31%; 114 clinicians, 91 trialists), the majority of respondents were interventionalists (86%) working in academic practices (72%). Although most physicians anticipated referring <25% of their patients with severe aortic stenosis for TAVR, 68% believed that TAVR is equally efficacious as open‐heart surgery, and 11% believed that moderate‐surgical risk patients should also be considered for TAVR. More clinicians (98%) than trialists (81%) expected to routinely refer patients for TAVR (P < 0.001). Furthermore, the clinicians were anticipating less operator training and lower annual volume requirements for performing TAVR, when compared with the trialists (P ≤ 0.001).ConclusionsOur findings suggest optimism for TAVR acceptance in the United States., with more conservative expectations regarding training, procedural volume requirements, and anticipated referral patterns among TAVR trialists than clinical interventionalists.
Structural Elaboration of a Natural Product: Identification of 3,3′‐Diindolylmethane Aminophosphonate and Urea Derivatives as Potent Anticancer Agents
Authors: Kandekar Somnath , Preet Ranjan , Kashyap Maneesh , M. U. Renu Prasad , Mohapatra Purusottam , Das Dipon , Satapathy Shakti Ranjan , Siddharth Sumit , Jain Vaibhav , Choudhuri Maitrayee , Kundu Chanakya N. , Guchhait Sankar K. , Bharatam Prasad V. 
Published: August 2013

AbstractAn approach involving rational structural elaboration of the biologically active natural product diindolylmethane (DIM) with the incorporation of aminophosphonate and urea moieties toward the discovery of potent anticancer agents was considered. A four‐step approach for the synthesis of DIM aminophosphonate and urea derivatives was established. These novel compounds showed potent anticancer activities in two representative kidney and colon cancer cell lines, low toxicity to normal cells, higher potency than the parent natural product DIM and etoposide, and potent inhibition of cancer cell migration. Biophysical and immunological studies, including DAPI nuclear staining, western blot analysis with apoptotic protein markers, flow cytometry, immunocytochemistry, and comet assays of the two most potent compounds revealed good efficacies in apoptosis and DNA damage. It was found that down‐regulation of nuclear factor κB (NF‐κB p65) could be an important mode of action in apoptosis, and the two most potent derivatives were found to be more potent than parent compound DIM in the down‐regulation of NF‐κB. Our results show the importance of structural elaboration of DIM by rational incorporation of aminophosphonate and urea moieties to produce potent anticancer agents; they also suggest that this approach using other structurally simple bioactive natural products as scaffolds holds promise for future drug discovery and development.
Precipitation and characterization of hollow calcite nanoparticles
Authors: Kontrec Jasminka , Ukrainczyk Marko , Džakula Branka Njegić , Kralj Damir 
Published: August 2013

AbstractA method for preparation of significant amount of hollow rhombohedral calcite nanoparticles, based on carbonation of calcium hydroxide suspension, is described. The mineralogical and morphological analyses of the precipitate confirmed the existence of exclusively stable polymorphic modification, calcite, with the mean particle size of about 100 nm and the diameter of the holes observed at the surfaces that are about 50 nm. The analysis of carbonation kinetics pointed out to a complex mechanism of hollow particles formation at high initial supersaturation, that assumed nucleation of amorphous precursor calcium carbonate phase and its solution mediated transformation into nanosized crystalline calcite. The holes obtained at the calcite surfaces are most probably the imprints remained after the dissolution of amorphous calcium carbonate particles.
Tissue specific oxidative stress profile in relation to glycaemic regulation in mice
Authors: Modak Manisha A. , Parab P. B. , Ghaskadbi Saroj S. 
Published: August 2013

AbstractBackgroundDiabetes mellitus is a metabolic disorder characterized by hyperglycaemia resulting from uncontrolled glucose regulation. Reactive oxygen species are recognized as one link between hyperglycaemia and diabetic complications. Studies have shown that diabetes mellitus is associated with decreases in antioxidant potential and increased formation of free radicals leading to oxidative stress. The present study was undertaken because an unequivocal demonstration that control of hyperglycaemia can reduce oxidative stress is still lacking.MethodsIn the present study, we investigated oxidative stress profile of normal, streptozotocin‐induced diabetic, insulin‐treated and untreated diabetic animals. On the one hand, oxidative damage caused to lipids, proteins and DNA was measured. On other hand, antioxidant defense was measured in terms of specific activities of antioxidant enzymes (AOEs) and antioxidant molecules.ResultsIt was observed that the damage to lipids, proteins and DNA caused by free radicals increased in diabetic animals compared with that in controls. In diabetic animals not treated with insulin, damage to all biological molecules increased further significantly (p ≤ 0.005). Changes in AOEs from different tissues were complex depicting a varied AOE level in different tissues. Insulin treatment significantly improved the oxidative stress profile in all tissues studies.ConclusionsThe control of hyperglycaemia improves oxidative stress profile, that is, the ability of cells to cope up with oxidative stress. Copyright © 2013 John Wiley & Sons, Ltd.
Installation of a Chiral Side Chain to a 2‐Alkylidene‐1‐cycloalkan‐1‐ol Unit by Using Allylic Substitution
Authors: Feng Chao , Kobayashi Yuichi 
Published: August 2013

AbstractThe allylic substitution of optically active exocyclic allylic esters of cyclopentane and cyclohexane with ArMgBr‐based copper reagents (Ar = aryl) was examined. ArMgBr/Cu(acac)2 in a 2:1 ratio was an adequate reagent to produce the anti‐SN2′ products efficiently in terms of regioselectivity (95–99 %), chirality transfer (91–99 %), and yield (71–91 %). The Ar groups that were successfully installed include Ph and p‐tolyl, those with electron‐donating (i.e., p‐MeOC6H4) and electron‐withdrawing groups (i.e., p‐FC6H4), and those with sterically demanding groups (i.e., o‐tolyl, o‐MeOC6H4). In an examination of an alkyl reagent, BuMgBr/CuBr·Me2S in a 2:1 ratio in the presence of ZnI2 afforded the product with high regioselectivity (99 %) and in good yield (91 %).
Detection ofListeria Monocytogenesby Electrochemical Impedance Spectroscopy
Authors: Radhakrishnan Rajeswaran , Jahne Michael , Rogers Shane , Suni Ian I. 
Published: August 2013

AbstractListeria monocytogenesis detected by electrochemical impedance spectroscopy using a mouse monoclonal antibody immobilized onto an Au electrode. This yields sensitivities of 0.825 kΩ cm2/(CFU/mL) and 1.129 kΩ cm2/(CFU/mL) and detection limits of 5 CFU/mL and 4 CFU/mL for ideal solutions and filtered tomato extract, respectively. Control experiments with an Au electrode onto which a mouse monoclonal antibody to GAPDH is immobilized demonstrate that non‐specific adsorption is insignificant for the system and methodology studied here. Control experiments withSalmonella entericademonstrate no cross‐reactivity to this food pathogen. Potential technological hurdles to development of a multiplexed impedance biosensor for food pathogens are also discussed.
Sensitive Voltammetric Determination of Baicalein at Thermally Reduced Graphene Oxide Modified Glassy Carbon Electrode
Authors: Zhang Dongdong , Zhang Yanmin , He Langchong 
Published: August 2013

AbstractThis work presents a sensitive voltammetric method for determination of the flavonoid baicalein by using a thermally reduced graphene oxide (TRGO) modified glassy carbon electrode (GCE) in 100 mM KCl‐10 mM sodium phosphate buffer solution (pH 7.40). The surface morphology and structure of TRGO investigated by atomic force microscopy, FT‐IR spectroscopy and Raman spectroscopy reveal that the TRGO prepared maintained as single or bilayer sheets and with significant edge‐plane‐like defect sites. The TRGO/GCE modified electrode shows more favorable electron transfer kinetics for potassium ferricyanide and potassium ferrocyanide probe molecules, which are important electroactive compounds, compared with bare GCE and GO/GCE electrodes. The electrochemical behaviors of baicalein at the TRGO/GCE were investigated by cyclic voltammetry, suggesting that the TRGO/GCE exhibits excellent electrocatalytic activity to baicalein. Under physiological conditions, the modified electrode showed linear voltammetric response from 10 nM to 10 µM for baicalein, with a detection limit of 6.0 nM. This work demonstrates that the graphene‐modified electrode is a promising tool for electrochemical determination of flavonoid drugs.
A Novel Approach to Monitoring of the Diffusion Junction Potential in Speciation Studies by Polarography Under very Acidic Conditions. Part I: The Reversible Cd(II)‐Picolinic Acid System
Authors: Billing Caren , Cukrowski Ignacy , Jordan Blake 
Published: August 2013

AbstractA new polarographic procedure has been developed for the study of metal‐ligand equilibria at pH<2 by applying an in situ monitoring of the diffusion junction potential by introducing a noncomplexing witness metal ion, Tl(I). As a case study, this procedure was applied to the Cd(II)‐picolinic acid (pyridine‐2‐carboxylic acid) system; DCTAST polarography was employed at 25 °C and ionic strength of 0.25–0.5 M (H,Na)NO3. Log β values of 4.26±0.03, 7.86±0.11 and 10.47±0.12 were obtained for the ML, ML2 and ML3 complexes, respectively, and compare well to literature values. The first log β (6.27±0.07) value for the MLH species was successfully determined, for which a crystal structure has been reported recently.
A Novel Electrochemical Method for the Analysis of Hydrogen Peroxide with the Use of a Glassy Carbon Electrode Modified by a Prussian Blue/Copper‐Gold Bimetallic Nanoparticles Hybrid Film
Authors: Ni Yongnian , Song Haiyan , Kokot Serge 
Published: August 2013

AbstractA novel Prussian blue/copper‐gold bimetallic nanoparticles hybrid film modified electrode was prepared by electrochemical deposition on a glassy carbon electrode (PB/Cu‐AuNPs/GCE). Morphology and electrochemistry of this electrode were studied by UV‐vis spectroscopy, scanning electron microscopy, X‐ray diffraction, cyclic voltammetry and electrochemical impedance spectroscopy. The sensor showed significantly better electrocatalytic activity for the reduction of hydrogen peroxide in comparison with the single PB/GCE and PB/AuNPs/GCE. This was attributed to the synergistic effect of PB and Cu‐Au bimetallic nanoparticles. Also, the sensor demonstrated an overall high level of performance for the analysis of H2O2 in the concentration range from 0.002 to 0.84 mM.