bluuebunny/crossref_metadata_2025_split huggingface dataset:
The immunosuppressive signature of menstrual blood mesenchymal stem cells entails opposite effects on experimental arthritis and graft versus host diseases
Authors: Luz-Crawford Patricia , Torres Maria J. , Noël Daniele , Fernandez Ainoa , Toupet Karine , Alcayaga-Miranda Francisca , Tejedor Gautier , Jorgensen Christian , Illanes Sebastian E. , Figueroa Fernando E. , Djouad Farida , Khoury Maroun 
Published: November 2015

Abstract
Recently, a noninvasive and highly proliferative stem cell population from menstrual blood called MenSCs has been identified. Despite their use in clinical studies, their immunomodulatory properties have not yet been investigated. In this context, we studied the immunosuppressive properties of MenSCs in comparison with the well-characterized bone marrow derived-MSCs (BM-MSCs). Using an in vitro proliferation assays, we showed that MenSCs displayed a lower suppressive effect on peripheral blood mononuclear cells and in particular on the proinflammatory CD4+IFN-γ+ and CD8+IFNγ+ cells than BM-MSCs. Moreover, compared to BM-MSCs, MenSCs activated with IFN-γ and IL-1β produced lower amounts of immunosuppressive factors such as IDO, PDL-1, PGE2, and Activin A and exhibited a substantial lower expression level of IFN-γ receptor subunits. In the collagen induced arthritis model, while BM-MSCs administration resulted in a potent therapeutic effect associated with a significant decrease of proinflammatory T cell frequency in the lymph nodes, MenSCs injection did not. In contrast, in the xeno-GVHD model, only MenSCs administration significantly increased the survival of mice. This beneficial effect mediated by MenSCs was associated with a higher capacity to migrate into the intestine and liver and not to their anti-inflammatory capacities. All together our results demonstrate for the first time that the therapeutic potential of MSC in the experimental xeno-GVHD model is independent of their immunosuppressive properties. These findings should be taken into consideration for the development of safe and effective cell therapies.
A Photoluminescent Metal Coordination Complex Constructed from Hydrothermal in situ Generated Quinoline‐monoacyl­hydrazidate Ligand
Authors: Yang Qing‐Feng , Bai Hong‐Cun , Li Bing , Luo Min , Jin Juan , Yu Jie‐Hui , Xu Ji‐Qing 
Published: November 2015

AbstractThe quinoline‐monoacylhydrazidate‐coordinated transition‐metal compound Cd(QLH)2(H2O)2 (1) (QLH = 2,3‐quinoline dicarboxylhydrazidate) was obtained by the hydrothermal in situ acylation reaction between 2,3‐quinoline dicarboxylic acid and hydrazine hydrate. X‐ray single‐crystal diffraction analysis reveals that compound 1 features a mononuclear structure. Through the O–H···O and C–H···O hydrogen bonds between the neighboring molecules, the mononuclear units of 1 aggregate together into a 3D supramolecular network, which is stabilized by offset face‐to‐face π···π interactions. Compound 1 was characterized by IR spectroscopy, elemental analysis, TGA, and photoluminescence analysis, and it shows good photoluminescent property in the solid state at room temperature. In order to understand the emission mechanism, DFT calculations were carried out on the excited electronic states of compound 1.
Understanding identity integration: Theoretical, methodological, and applied issues
Authors: Syed Moin , McLean Kate C. 
Published: November 2015

ABSTRACTIdentity integration is one of the foundational theoretical concepts in Erikson's (1968) theory of lifespan development. However, the topic is understudied relative to its theoretical and practical importance. The extant research is limited in quantity and scope, and there is considerable heterogeneity in how identity integration is conceptualized and measured. Accordingly, the purpose of this paper is to 1) provide a conceptual discussion of different forms of identity integration 2) highlight the different methodological approaches represented in the literature, and 3) detail the implications of integration for psychological functioning. In particular, we provide a conceptual and methodological discussion of four forms of integration: two that are widely recognized, contextual integration and temporal integration, and two that have received less attention, ego integration and person‐society integration. We see this paper as filling a need in the literature for those interested in how complex identity processes are related to psychological functioning.
Early life trauma is associated with decreased peripheral levels of thyroid‐hormone T3 in adolescents
Authors: Machado T.D. , Salum G.A. , Bosa V.L. , Goldani M.Z. , Meaney M.J. , Agranonik M. , Manfro G.G. , Silveira P.P. 
Published: November 2015

AbstractAn adverse early life environment can induce changes on behavioral and metabolic responses later in life. Recent studies in rats showed that the quality of maternal care as measured by high levels of pup licking and grooming (LG) was associated with changes in the relationship between the precursor thyroid‐hormone T4 and the more active T3. Here we investigated if early exposure to childhood abuse is associated with thyroid‐hormone levels in human adolescents. Given the empirical evidence from animal models showing that good maternal care was associated with increased conversion of T4 to T3, we hypothesized that early adversity would be associated with a decreased peripheral conversion of T4 to T3. A sample of 80 adolescents (10–18 years) participated in this study. We used the Childhood Trauma Questionnaire to investigate early life stress. We calculate the body mass index (BMI) assessing weight and height and sexual maturation stage was determined by self‐assessment. Blood samples were collected to measure T3 and T4 levels. ANCOVA were used to evaluate the influence of the Physical Abuse domain of the Childhood Trauma Questionnaire as the early life stress variable in T3 and T4 separately, adjusted for potential confounders such as pubertal status, gender, socioeconomic status and BMI. Early life trauma was associated with reduced T3 levels in adolescents, when adjusted for potential confounders (p = 0.013), but not with peripheral T4 levels (p = 0.625). We extended findings from animal models showing that adverse early experience persistently impacts on the individual's responses to stress, which is marked by an abnormal metabolism of thyroid hormones. Further studies are needed to further investigate the nature of such associations.
Development of a circulating miRNA assay to monitor tumor burden: From mouse to man
Authors: Greystoke Alastair , Ayub Mahmood , Rothwell Dominic G. , Morris Dan , Burt Deborah , Hodgkinson Cassandra L. , Morrow Christopher J. , Smith Nigel , Aung Kyaw , Valle Juan , Carter Louise , Blackhall Fiona , Dive Caroline , Brady Ged 
Published: November 2015

Circulating miRNA stability suggests potential utility of miRNA based biomarkers to monitor tumor burden and/or progression, particularly in cancer types where serial biopsy is impractical. Assessment of miRNA specificity and sensitivity is challenging within the clinical setting. To address this, circulating miRNAs were examined in mice bearing human SCLC tumor xenografts and SCLC patient derived circulating tumor cell explant models (CDX). We identified 49 miRNAs using human TaqMan Low Density Arrays readily detectable in 10 μl tail vein plasma from mice carrying H526 SCLC xenografts that were low or undetectable in non‐tumor bearing controls. Circulating miR‐95 measured serially in mice bearing CDX was detected with tumor volumes as low as 10 mm3 and faithfully reported subsequent tumor growth.Having established assay sensitivity in mouse models, we identified 26 miRNAs that were elevated in a stage dependent manner in a pilot study of plasma from SCLC patients (n = 16) compared to healthy controls (n = 11) that were also elevated in the mouse models. We selected a smaller panel of 10 previously reported miRNAs (miRs 95, 141, 200a, 200b, 200c, 210, 335#, 375, 429) that were consistently elevated in SCLC, some of which are reported to be elevated in other cancer types. Using a multiplex qPCR assay, elevated levels of miRNAs across the panel were also observed in a further 66 patients with non‐small cell lung, colorectal or pancreatic cancers. The utility of this circulating miRNA panel as an early warning of tumor progression across several tumor types merits further evaluation in larger studies.
Emergency Department Catheter-Associated Urinary Tract Infection Prevention: Multisite Qualitative Study of Perceived Risks and Implemented Strategies
Authors: Carter Eileen J. , Pallin Daniel J. , Mandel Leslie , Sinnette Corine , Schuur Jeremiah D. 
Published: November 2015

BACKGROUNDExisting knowledge of emergency department (ED) catheter-associated urinary tract infection (CAUTI) prevention is limited. We aimed to describe the motivations, perceived risks for CAUTI acquisition, and strategies used to address CAUTI risk among EDs that had existing CAUTI prevention programs.METHODSIn this qualitative comparative case study, we enrolled early-adopting EDs, that is, those using criteria for urinary catheter placement and tracking the frequency of catheters placed in the ED. At 6 diverse facilities, we conducted 52 semistructured interviews and 9 focus groups with hospital and ED participants.RESULTSAll ED CAUTI programs originated from a hospitalwide focus on CAUTI prevention. Staff were motivated to address CAUTI because they believed program compliance improved patient care. ED CAUTI prevention was perceived to differ from CAUTI prevention in the inpatient setting. To identify areas of ED CAUTI prevention focus, programs examined ED workflow and identified 4 CAUTI risks: (1) inappropriate reasons for urinary catheter placement; (2) physicians’ limited involvement in placement decisions; (3) patterns of urinary catheter overuse; and (4) poor insertion technique. Programs redesigned workflow to address risks by (1) requiring staff to specify the medical reason for catheter at the point of order entry and placement; (2) making physicians responsible for determining catheter use; (3) using catheter alternatives to address patterns of overuse; and (4) modifying urinary catheter insertion practices to ensure proper placement.CONCLUSIONSEarly-adopting EDs redesigned workflow to minimize catheter use and ensure proper insertion technique. Assessment of ED workflow is necessary to identify and modify local practices that may increase CAUTI risk.Infect. Control Hosp. Epidemiol. 2016;37(2):156–162
JUSTICE, EQUALITY, AND NATURAL RIGHTS CLAIMS: A RECONSIDERATION OF AQUINAS'S CONCEPTION OF RIGHT
Authors: Porter Jean 
Published: November 2015

AbstractAccording to a widely held view, Aquinas does not have a notion of subjective natural rights, understood as moral powers inhering in individuals. This article argues that this way of reading Aquinas is wrong, or at best, seriously misleading. Aquinas does identify the right, the object of justice, with the relation established between parties to an equitable exchange or interaction, and in this sense he identifies right with an objective state of affairs. But this line of analysis does not commit him to any particular construal of what constitutes a just relation. In particular, it leaves open the possibility that in some situations, the right, understood as an objectively equitable relation, presupposes that someone's claim of a right, is duly acknowledged. Moreover, in many contexts Aquinas says that individuals can claim certain liberties and immunities on the basis of some natural right, in terms that make it clear that these claims lie within the discretion of the individual. His overall conception of natural law and natural right implies that individuals can legitimately make certain claims by right, claims that emerge within some contexts and not others. He does not have a theory of rights, but neither do the scholastic jurists of the time, and his appeals to what someone can claim by right are reminiscent of their views. If they can be said to have a notion of subjective natural rights, the same can be said of Aquinas himself.
Production improvement through phosphorus fertilization and legume introduction in grazed native pastures of Uruguay
Authors: DEL PINO A. , RODRÍGUEZ T. , ANDIÓN J. 
Published: November 2015

SUMMARYThe objective of the current study was to quantify the response of pasture to phosphorus (P) fertilizer application and legume introduction, by measuring herbage yield, nitrogen (N) and P content, and weight gain of calves in native pastures of Uruguay. Quantitative relationships between pasture characteristics and post-weaning daily live weight gain (DLWG) were also examined. The treatments studied were native grassland (NG) and improved pasture, oversown withLotus corniculatusL. andTrifolium repensL. with annual applications of either 13 and 26 kg P/ha. From 1996 to 2001 the treatments were evaluated each year with a new group of calves. Total herbage yields of the oversown pastures were not always higher than NG in the initial years, but legume production increased, although without significant differences between P rates on legume or total yield. This was also reflected in the N and P status of the swards. In the last 2 years legume proportion had declined to <0·1, but total herbage yield was significantly higher in the improved pastures. The average DLWG over the 6 years of measurements were 0·319, 0·478 and 0·586 kg/day for NG, P1 and P2, respectively, with average total live weight gain increased 1·8- and 2·5-fold by the legume introduction and annual addition of 13 and 26 kg P/ha, respectively. The study demonstrated that the evaluation of pasture response to P application should not be limited to assessing forage yield increase. Phosphorus availability in the herbage was a better predictor of animal performance than pasture yield. The study highlights that the benefits of oversowing and fertilizer inputs are short-lived, as withholding of fertilizer in the last 2 years resulted in a loss of sown legumes and decline in animal production.
In vitro trichomonacidal activity and preliminary in silico chemometric studies of 5-nitroindazolin-3-one and 3-alkoxy-5-nitroindazole derivatives
Authors: IBÁÑEZ-ESCRIBANO ALEXANDRA , NOGAL-RUIZ JUAN JOSÉ , GÓMEZ-BARRIO ALICIA , ARÁN VICENTE J. , ESCARIO JOSÉ ANTONIO 
Published: November 2015

SUMMARYA selection of 1,2-disubstituted 5-nitroindazolin-3-ones (1–19) and 3-alkoxy-5-nitroindazoles substituted at positions 1 (20–24) or 2 (25–39) from our in-house compound library were screened in vitro against the most common curable sexually transmitted pathogen, Trichomonas vaginalis. A total of 41% of the studied molecules (16/39) achieved a significant activity of more than 85% growth inhibition at the highest concentration assayed (100 µg mL−1). Among these compounds, 3-alkoxy-5-nitroindazole derivatives 23, 24, 25 and 27 inhibited parasite growth by more than 50% at 10 µg mL−1. In addition, the first two compounds (23, 24) still showed remarkable activity at the lowest dose tested (1 µg mL−1), inhibiting parasite growth by nearly 40%. Their specific activity towards the parasite was corroborated by the determination of their non-specific cytotoxicity against mammalian cells. The four mentioned compounds exhibited non-cytotoxic profiles at all of the concentrations assayed, showing a fair antiparasitic selectivity index (SI > 7·5). In silico studies were performed to predict pharmacokinetic properties, toxicity and drug-score using Molinspiration and OSIRIS computational tools. The current in vitro results supported by the virtual screening suggest 2-substituted and, especially, 1-substituted 3-alkoxy-5-nitroindazoles as promising starting scaffolds for further development of novel chemical compounds with the main aim of promoting highly selective trichomonacidal lead-like drugs with adequate pharmacokinetic and toxicological profiles.
No progressive brain changes during a 1-year follow-up of patients with first-episode psychosis
Authors: Haukvik U. K. , Hartberg C. B. , Nerland S. , Jørgensen K. N. , Lange E. H. , Simonsen C. , Nesvåg R. , Dale A. M. , Andreassen O. A. , Melle I. , Agartz I. 
Published: November 2015

BackgroundFirst-episode psychosis (FEP) patients show structural brain abnormalities. Whether the changes are progressive or not remain under debate, and the results from longitudinal magnetic resonance imaging (MRI) studies are mixed. We investigated if FEP patients showed a different pattern of regional brain structural change over a 1-year period compared with healthy controls, and if putative changes correlated with clinical characteristics and outcome.MethodMRIs of 79 FEP patients [SCID-I-verified diagnoses: schizophrenia, psychotic bipolar disorder, or other psychoses, mean age 27.6 (s.d. = 7.7) years, 66% male] and 82 healthy controls [age 29.3 (s.d. = 7.2) years, 66% male] were acquired from the same 1.5 T scanner at baseline and 1-year follow-up as part of the Thematically Organized Psychosis (TOP) study, Oslo, Norway. Scans were automatically processed with the longitudinal stream in FreeSurfer that creates an unbiased within-subject template image. General linear models were used to analyse longitudinal change in a wide range of subcortical volumes and detailed thickness and surface area estimates across the entire cortex, and associations with clinical characteristics.ResultsFEP patients and controls did not differ significantly in annual percentage change in cortical thickness or area in any cortical region, or in any of the subcortical structures after adjustment for multiple comparisons. Within the FEP group, duration of untreated psychosis, age at illness onset, antipsychotic medication use and remission at follow-up were not related to longitudinal brain change.ConclusionsWe found no significant longitudinal brain changes over a 1-year period in FEP patients. Our results do not support early progressive brain changes in psychotic disorders.
Computerized lexis-based instruction in EFL classrooms: Using multi-purpose LexisBOARD to teach L2 vocabulary
Authors: Mirzaei Azizullah , Rahimi Domakani Masoud , Rahimi Sedigheh 
Published: November 2015

AbstractLexis-based views of second or foreign language (L2) teaching place prime importance on the teaching of conventionalized multi-word lexical items, or unanalyzed chunks, as a useful mechanism for fostering learners’ creative production of forms and their subsequent development of L2 competence. This pretest/posttest quasi-experimental study probed the use of teacher-designed multi-purpose instructional lexis software, dubbed LexisBOARD, on L2 learners’ vocabulary achievement in an Iranian EFL (English as a foreign language) context. A cohort of 50 Iranian junior-high-school students participated in the main instructional phase of the study. Instruction on L2 lexical items (e.g., concordances, polywords, or formulaic sequences) was mainly given to the experimental group using LexisBOARD, which was designed to be user-friendly and attuned to learners’ communicative and curricular needs. LexisBOARD offered further practice or feedback affordances through engaging students in lexical exercises (with word partnerships and collocations) for each unit and several quizzes for self-assessment. The control group was only taught using their mainstream EFL textbooks focusing on grammatical rules, discrete vocabulary items with fixed meanings, and reading texts, without any use of corpus-based activities. The results of the groups’ vocabulary test scores indicate that the lexis group significantly outperformed the control group, pointing to the superiority of practicing and learning L2 vocabulary when lexical items are seen in larger, more holistic ways and, especially, when engaging and experimenting with lexis is scaffolded through computer affordances.
Experimental resource pulses influence social-network dynamics and the potential for information flow in tool-using crows
Authors: St Clair James J. H. , Burns Zackory T. , Bettaney Elaine M. , Morrissey Michael B. , Otis Brian , Ryder Thomas B. , Fleischer Robert C. , James Richard , Rutz Christian 
Published: November 2015

AbstractSocial-network dynamics have profound consequences for biological processes such as information flow, but are notoriously difficult to measure in the wild. We used novel transceiver technology to chart association patterns across 19 days in a wild population of the New Caledonian crow—a tool-using species that may socially learn, and culturally accumulate, tool-related information. To examine the causes and consequences of changing network topology, we manipulated the environmental availability of the crows’ preferred tool-extracted prey, and simulated, in silico, the diffusion of information across field-recorded time-ordered networks. Here we show that network structure responds quickly to environmental change and that novel information can potentially spread rapidly within multi-family communities, especially when tool-use opportunities are plentiful. At the same time, we report surprisingly limited social contact between neighbouring crow communities. Such scale dependence in information-flow dynamics is likely to influence the evolution and maintenance of material cultures.
Basal forebrain control of wakefulness and cortical rhythms
Authors: Anaclet Christelle , Pedersen Nigel P. , Ferrari Loris L. , Venner Anne , Bass Caroline E. , Arrigoni Elda , Fuller Patrick M. 
Published: November 2015

AbstractWakefulness, along with fast cortical rhythms and associated cognition, depend on the basal forebrain (BF). BF cholinergic cell loss in dementia and the sedative effect of anti-cholinergic drugs have long implicated these neurons as important for cognition and wakefulness. The BF also contains intermingled inhibitory GABAergic and excitatory glutamatergic cell groups whose exact neurobiological roles are unclear. Here we show that genetically targeted chemogenetic activation of BF cholinergic or glutamatergic neurons in behaving mice produced significant effects on state consolidation and/or the electroencephalogram but had no effect on total wake. Similar activation of BF GABAergic neurons produced sustained wakefulness and high-frequency cortical rhythms, whereas chemogenetic inhibition increased sleep. Our findings reveal a major contribution of BF GABAergic neurons to wakefulness and the fast cortical rhythms associated with cognition. These findings may be clinically applicable to manipulations aimed at increasing forebrain activation in dementia and the minimally conscious state.
The paracaspase MALT1 cleaves HOIL1 reducing linear ubiquitination by LUBAC to dampen lymphocyte NF-κB signalling
Authors: Klein Theo , Fung Shan-Yu , Renner Florian , Blank Michael A. , Dufour Antoine , Kang Sohyeong , Bolger-Munro Madison , Scurll Joshua M. , Priatel John J. , Schweigler Patrick , Melkko Samu , Gold Michael R. , Viner Rosa I. , Régnier Catherine H. , Turvey Stuart E. , Overall Christopher M. 
Published: November 2015

AbstractAntigen receptor signalling activates the canonical NF-κB pathway via the CARD11/BCL10/MALT1 (CBM) signalosome involving key, yet ill-defined roles for linear ubiquitination. The paracaspase MALT1 cleaves and removes negative checkpoint proteins, amplifying lymphocyte responses in NF-κB activation and in B-cell lymphoma subtypes. To identify new human MALT1 substrates, we compare B cells from the only known living MALT1mut/mut patient with healthy MALT1+/mut family members using 10-plex Tandem Mass Tag TAILS N-terminal peptide proteomics. We identify HOIL1 of the linear ubiquitin chain assembly complex as a novel MALT1 substrate. We show linear ubiquitination at B-cell receptor microclusters and signalosomes. Late in the NF-κB activation cycle HOIL1 cleavage transiently reduces linear ubiquitination, including of NEMO and RIP1, dampening NF-κB activation and preventing reactivation. By regulating linear ubiquitination, MALT1 is both a positive and negative pleiotropic regulator of the human canonical NF-κB pathway—first promoting activation via the CBM—then triggering HOIL1-dependent negative-feedback termination, preventing reactivation.