bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hemoglobin Alc Testing is Associated with Improved Pancreas Utilization for Transplant
Authors: Torres-Quinones Maritza , Stern Jeffrey , Seals Nicholas , Giglio Gysel , Meza Kathryn , Carpini Kristin Delli , Icitovic Nikolina , Tajik Waheed , O'Hara David , Lerner Harvey , Goldstein Michael J. 
Published: March 2013

Context Aging, higher prevalence of diabetes, worsening obesity, and hyperglycemia among potential donors increase the likelihood that pancreata will be declined by transplant centers. Hemoglobin Alc testing, also known as glycated hemoglobin testing, identifies a donor's average blood glucose concentration for the preceding 2 to 3 months and is the standard test for identifying prolonged periods of hyperglycemia. Objective To compare pancreas utilization rates before and after implementation of hemoglobin Alc testing. Design A retrospective study of data from the New York Organ Donor Network was conducted. Potential donors were defined as standard criteria donors who had no history of diabetes and were not seropositive for hepatitis B or C. Criteria for “ideal” potential pancreas donors were based on age, body mass index, lipase level, and terminal creatinine level. Potential donors who did not meet the criteria for ideal donors were considered “expanded” potential pancreas donors. Pancreas utilization rate was defined as the number of pancreata transplanted divided by the number of potential pancreas donors. Results Of 779 standard criteria donors, 691 (89%) were potential pancreas donors: 251 ideal (36%) and 440 expanded (64%) donors. In 2005 and 2006, before hemoglobin Alc testing, pancreas utilization rates were 21% and 18%, respectively. In 2008, 2009, and 2010, after hemoglobin Alc testing was incorporated, utilization rates were 27%, 28%, and 32%, respectively. Utilization of ideal donors increased from 33% to 51% ( P = .003), and utilization of expanded donors increased from 11% to 17% ( P = .05). Pancreas utilization increased 51.0%, and pancreas discards decreased 50.8% with the implementation of hemoglobin Alc testing. Conclusion Hemoglobin Alc testing may increase utilization of ideal and expanded criteria pancreata.
Photoactivated Localization Microscopy (PALM) of Adhesion Complexes
Authors: Shroff Hari , White Helen , Betzig Eric 
Published: March 2013

AbstractKey to understanding a protein's biological function is the accurate determination of its spatial distribution inside a cell. Although fluorescent protein markers allow the targeting of specific proteins with molecular precision, much of this information is lost when the resultant fusion proteins are imaged with conventional, diffraction‐limited optics. In response, several imaging modalities that are capable of resolution below the diffraction limit (∼200 nm) have emerged. Here, both single‐ and dual‐color superresolution imaging of biological structures using photoactivated localization microscopy (PALM) are described. The examples discussed focus on adhesion complexes: dense, protein‐filled assemblies that form at the interface between cells and their substrata. A particular emphasis is placed on the instrumentation and photoactivatable fluorescent protein (PA‐FP) tags necessary to achieve PALM images at ∼20 nm resolution in 5 to 30 min in fixed cells. Curr. Protoc. Cell Biol. 58:4.21.1‐4.21.28. © 2013 by John Wiley & Sons, Inc.
Chromatin Immunoprecipitation of Adult Murine Cardiomyocytes
Authors: Bolli P. , Vardabasso C. , Bernstein E. , Chaudhry H.W. 
Published: March 2013

AbstractThis unit describes a streamlined two‐step protocol for the isolation of adult murine cardiomyocytes with subsequent Chromatin ImmunoPrecipitation (ChIP). Isolation and culturing of cardiomyocytes is a delicate process and the protocol presented here optimizes the combination of cardiomyocyte isolation with ChIP. ChIP is an invaluable method for analyzing molecular interactions occurring between a specific protein (or its post‐translationally modified form) and a region of genomic DNA. ChIP has become a widely used technique in the last decade since several groundbreaking studies have focused attention on epigenetics and have identified many epigenetic regulatory mechanisms. However, epigenetics within cardiovascular biology is a new area of focus for many investigators, and we have optimized a method for performing ChIP in adult murine cardiomyocytes, as we feel this will be an important aid to both the cardiovascular field and for the development of cell‐ and tissue‐specific ChIP. Curr. Protoc. Cell Biol. 58:17.14.1‐17.14.16. © 2013 by John Wiley & Sons, Inc.
A Near‐Infrared cis‐Configured Squaraine Co‐Sensitizer for High‐Efficiency Dye‐Sensitized Solar Cells
Authors: Qin Chuanjiang , Numata Youhei , Zhang Shufang , Islam Ashraful , Yang Xudong , Sodeyama Keitaro , Tateyama Yoshitaka , Han Liyuan 
Published: March 2013

AbstractA cis‐configured squaraine dye (HSQ1), synthesized by incorporation of a strongly electron‐withdrawing dicyanovinyl group into the central squaric acid moiety, is employed in dye‐sensitized solar cells (DSCs). In solution, HSQ1 displays an intense absorption in the near‐infrared region with a maximum at 686 nm and when the dye is adsorbed on a TiO2 surface, the absorption spectrum broadens in both the blue and the near‐infrared regions, which is favorable for efficient light harvesting over a broad wavelength range. A solar cell sensitized with HSQ1 shows a broader incident photon‐to‐current conversion efficiency (IPCE) spectrum (from 400 to 800 nm) and a higher IPCE in the long‐wavelength region (71% at 700 nm) than a cell sensitized with squaraine dye SQ1. Furthermore, a solar cell co‐sensitized with HSQ1 and N3 dye shows remarkably improved short‐circuit current density and open‐circuit voltage compared to those of a DSC based on N3 alone and fabricated under the same conditions. The energy‐conversion efficiency of the co‐sensitized DSC is 8.14%, which is the highest reported efficiency for a squaraine dye–based co‐sensitized DSC without using Al2O3 layer.
A mosaic 2q24.2 deletion narrows the critical region to a 0.4 Mb interval that includes TBR1, TANK, and PSMD14
Authors: Burrage Lindsay C. , Eble Tanya N. , Hixson Patricia M. , Roney Erin K. , Cheung Sau W. , Franco Luis M. 
Published: February 2013

AbstractInterstitial deletions involving 2q24 have been associated with a wide range of phenotypes including intellectual disability and short stature. To date, the smallest common region among reported cases of deletions in this region is approximately 2.65 Mb and contains 15 genes. In the present case report, we describe an 18‐year‐old male with mild intellectual disability, short stature, and mosaicism for a 0.422 Mb deletion on 2q24.2 that was diagnosed by comparative genomic hybridization and confirmed with fluorescent in situ hybridization (FISH). This deletion, which is present in approximately 61% of cells, includes three genes: TBR1, TANK, and PSMD14. The findings suggest that the critical region for intellectual disability and short stature in 2q24.2 can be narrowed to a 0.422 Mb segment. TBR1, a transcription factor involved in early cortical development, is a strong candidate for the intellectual disability phenotype seen in our patient and in patients with larger deletions in this region of the genome. © 2013 Wiley Periodicals, Inc.
Inversion upstream of FOXF1 in a case of lethal alveolar capillary dysplasia with misalignment of pulmonary veins
Authors: Parris Toshima , Nik Ali Moussavi , Kotecha Sailesh , Langston Claire , Helou Khalil , Platt Craig , Carlsson Peter 
Published: February 2013

AbstractAlveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a congenital malformation that leads to severe pulmonary hypertension and respiratory failure. It has been associated with deletion of, or mutation in, FOXF1 on 16q24.1, a gene encoding a forkhead transcription factor expressed in the mesenchyme of the developing lung. Here we report on the identification of a pericentric inversion on chromosome 16 (p11.2q24.1) in a case of lethal ACDMPV with atrioventricular septal defect and duodenal atresia. Array‐CGH indicated that the inversion is balanced, and FISH showed that the q‐arm breakpoint occurs 134 ± 10 kb upstream (5′; centromeric) of FOXF1. This is suggestive of cis‐regulatory elements located more than 130 kb 5′ of FOXF1, and analysis of genome‐wide data sets of chromatin modifications in two different cell types suggested that the FOXF1 regulatory domain covers more than 300 kb, and perhaps up to 433 kb, upstream of the gene, but only 3 kb downstream. The 588 kb gene‐free region between FOXF1 and the next gene in the centromeric direction, IRF8, is highly conserved between species and divided into two distinct regulatory domains by an insulator element. Another putative insulator occurs just downstream of FOXF1. Our results further strengthen the association between FOXF1 and a spectrum of malformations that include ACDMPV, atrioventricular septal defects, and gastrointestinal atresia. Furthermore, the presented analysis aids in defining the critical genomic region for this syndrome. © 2013 Wiley Periodicals, Inc.
Daughter dearest: Sex‐biased calcium in mother's milk among rhesus macaques
Authors: Hinde Katie , Foster Alison B. , Landis Lauren M. , Rendina Danielle , Oftedal Olav T. , Power Michael L. 
Published: March 2013

ABSTRACTMother's milk provides building blocks necessary for infant development and growth postnatally. Minerals in milk are particularly important for infant skeletal development and may reflect maternal characteristics that are associated with the capacity to synthesize milk and sex‐specific developmental priorities of the infant. Using a large sample of mother–infant dyads assigned to the outdoor breeding colony at the California National Primate Research Center (N=104), we investigated the relationship of milk calcium (Ca) and phosphorus (P) concentrations and the ratio ofCa/Pto maternal and infant characteristics and to other milk variables.CaandPare largely associated with casein micelles, and as expected, bothCaandPwere positively correlated with protein concentrations in milk. NeitherCanorPconcentrations were associated with maternal parity. Mothers rearing daughters tended to produce higher meanCaconcentration in milk, and consequently a higherCa/Pratio, than did mothers rearing sons, even though protein concentration was not elevated. These results suggest that theCa/Pratio in rhesus milk may have been under separate selective pressure from protein content to facilitate the accelerated rate of skeletal calcification that has been observed in femaleMacaca mulattainfants. Am J Phys Anthropol 151:144–150, 2013. © 2013 Wiley Periodicals, Inc.
Effect of the mass ratio of Fe3O4/PAA on separation properties and the extractive amount of Fe3O4 during the formation of Fe3O4‐PES ultrafiltration membranes
Authors: Huang Zheng‐Qing , Chen Ya‐Shu , Chen Zhi‐Wei , Nie Long‐Hui , Zhang Zhi , Xu Hong‐Tao , Zhou Kai‐Mei 
Published: March 2013

AbstractA series of Fe3O4‐PES ultrafiltration membranes with different mass ratios of Fe3O4 and PAA were prepared from suspensions, using the phase inversion process. The suspensions consisted of polyether sulfone (PES), dimethyl formamide, polyacrylic acid (PAA), and ferrosoferric oxide (Fe3O4). The separation properties of ultrafiltration membranes with different Fe3O4/PAA mass ratio were investigated by a cross‐flow experimental system. The Fe3O4/PAA mass ratio had little effect on the rejection of membranes to BSA. However, the pure water flux had a slight decline and then rised rapidly with the increase of Fe3O4/PAA mass ratio. An interesting phenomenon observed was that the Fe3O4 particles could diffuse into the nonsolvent bath during the formation of membrane, and the amount of Fe3O4 extracted into the nonsolvent bath nearly kept a constant mass ratio to PAA, even if the Fe3O4/PAA proportion was changed. The reasons of this interesting phenomenon were investigated. This result indicates that modified inorganic fillers may be used as the pore‐forming agent to prepare the porous membranes like the template leaching method. At the same time, this method does not use any strong acid or base. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Brief Report: Susceptibility to Childhood‐Onset Rheumatoid Arthritis: Investigation of a Weighted Genetic Risk Score That Integrates Cumulative Effects of Variants at Five Genetic Loci
Authors: Prahalad Sampath , Conneely Karen N. , Jiang Yunxuan , Sudman Marc , Wallace Carol A. , Brown Milton R. , Ponder Lori A. , Rohani‐Pichavant Mina , Zwick Michael E. , Cutler David J. , Angeles‐Han Sheila T. , Vogler Larry B. , Kennedy Christine , Rouster‐Stevens Kelly , Wise Carol A. , Punaro Marilynn , Reed Ann M. , Mellins Elizabeth D. , Bohnsack John F. , Glass David N. , Thompson Susan D. 
Published: March 2013

ObjectiveChildren with childhood‐onset rheumatoid arthritis (RA) include those with rheumatoid factor or anti–citrullinated protein antibody–positive juvenile idiopathic arthritis. To test the hypothesis that adult‐onset RA–associated variants are also associated with childhood‐onset RA, we investigated RA‐associated variants at 5 loci in a cohort of patients with childhood‐onset RA. We also assessed the cumulative association of these variants in susceptibility to childhood‐onset RA using a weighted genetic risk score (wGRS).MethodsA total of 155 children with childhood‐onset RA and 684 healthy controls were genotyped for 5 variants in the PTPN22, TRAF1/C5, STAT4, and TNFAIP3 loci. High‐resolution HLA–DRB1 genotypes were available for 149 cases and 373 controls. We tested each locus for association with childhood‐onset RA via logistic regression. We also computed a wGRS for each subject, with weights based on the natural log of the published odds ratios (ORs) for the alleles investigated, and used logistic regression to test the wGRS for association with childhood‐onset RA.ResultsChildhood‐onset RA was associated with TNFAIP3 rs10499194 (OR 0.60 [95% confidence interval 0.44–0.83]), PTPN22 rs2476601 (OR 1.61 [95% confidence interval 1.11–2.31]), and STAT4 rs7574865 (OR 1.41 [95% confidence interval 1.06–1.87]) variants. The wGRS was significantly different between cases and controls (P < 2 × 10−16). Individuals in the third to fifth quintiles of wGRS had a significantly increased disease risk compared to baseline (individuals in the first quintile). Higher wGRS was associated with increased risk of childhood‐onset RA, especially among males.ConclusionThe magnitude and direction of the association between TNFAIP3, STAT4, and PTPN22 variants and childhood‐onset RA are similar to those observed in RA, suggesting that adult‐onset RA and childhood‐onset RA share common genetic risk factors. Using a wGRS, we have demonstrated the cumulative association of RA‐associated variants with susceptibility to childhood‐onset RA.
Evaluating the influence of selection markers on obtaining selected pools and stable cell lines in human cells
Authors: Lanza Amanda M. , Kim Do Soon , Alper Hal S. 
Published: March 2013

AbstractSelection markers are common genetic elements used in recombinant cell line development. While several selection systems exist for use in mammalian cell lines, no previous study has comprehensively evaluated their performance in the isolation of recombinant populations and cell lines. Here we examine four antibiotics, hygromycin B, neomycin, puromycin, and Zeocin™, and their corresponding selector genes, using a green fluorescent protein (GFP) as a reporter in two model cell lines, HT1080 and HEK293. We identify Zeocin™ as the best selection agent for cell line development in human cells. In comparison to the other selection systems, Zeocin™ is able to identify populations with higher fluorescence levels, which in turn leads to the isolation of better clonal populations and less false positives. Furthermore, Zeocin™‐resistant populations exhibit better transgene stability in the absence of selection pressure compared to other selection agents. All isolated Zeocin™‐resistant clones, regardless of cell type, exhibited GFP expression. By comparison, only 79% of hygromycin B‐resistant, 47% of neomycin‐resistant, and 14% of puromycin‐resistant clones expressed GFP. Based on these results, we rank Zeocin™ > hygromycin B ∼ puromycin > neomycin for cell line development in human cells. Furthermore, this study demonstrates that selection marker choice does indeed impact cell line development.
Investigating proline puckering states in diproline segments in proteins
Authors: Saha Indranil , Shamala Narayanaswamy 
Published: February 2013

ABSTRACTIn the current study, the puckering states of the Proline ring occurring in diproline segments (LPro‐LPro) in proteins has been investigated with a segregation made on the basis of cis and trans states for the Pro‐Pro peptide bond and the conformational states for the diproline segment to investigate the effects of conformation of the diproline segment on the corresponding puckering state of the Proline ring in the segment if any. The value of the endocyclic ring torsional angles of the pyrrolidine ring has been used for calculating and visualizing various puckering states using a proposed new sign convention (+/−) nomenclature. The results have been compared to that obtained in a previous study on peptides from this group. In this study, quite interestingly, the Planar (G) conformation that was present in 14.3% of the cases in peptides, appears to be nearly a rare conformation in the case of proteins (1.9%). The present study indicates that the (Cγ‐exo/Cγ‐exo), (Cγ‐exo/Twisted Cγ‐exo‐Cβ‐endo) and (Twisted Cγ‐endo‐Cβ‐exo/Twisted Cγ‐endo‐Cβ‐exo) categories are the most preferred combinations. For Proline rings in proteins, the states Cγ‐exo, Twisted Cγ‐exo‐Cβ‐endo and Twisted Cγ‐endo‐Cβ‐exo are the most preferred states. Within diproline segments, the pyrrolidine ring conformations do not show a strong co‐relation to the backbone conformation in which they are observed. It is likely that five‐membered rings have a considerable plasticity of structure and are readily deformed to accommodate a variety of energetically preferred backbone conformations. © 2013 Wiley Periodicals, Inc. Biopolymers 99: 605–610, 2013.
ASIS&T annual meeting pre‐conference activities: The history of ASIS&T and information science and technology
Authors: Miller Karen 
Published: February 2013

AbstractEditor's SummaryA highlight of the 2012 ASIS&T Annual Meeting, the pre‐conference session on the History of ASIS&T and Information Science and Technology Worldwide drew presenters and attendees from around the globe. The day featured papers on four historical themes, starting with the institutional roots of ASIS&T and recognizing decades of research presented in the Annual Review of Information Science and Technology. The evolution of the field was apparent through a review of information revolutions prompted by the printing press, the post‐World War II information crisis and the Internet, as well as through presentations on digital curation, ongoing work on relevance, sense‐making theory and developments from Croatia to France. Discussion of the historical contexts of technology innovations and impacts considered photographic documentary techniques, binary computing and networking standards. The development of foundational ideas was explored through presentations on pioneering document indexing methods, the semantic challenge of term‐oriented retrieval, early European perceptions of classification systems and the French view of communication and information science. Efforts to deepen the historical understanding of information science and technology will continue through oral history interviews, funded research and awards for outstanding papers.
Street Boys and Girls: A Case Study in Mauritania
Authors: Ballet Jérôme , Bhukuth Augendra , Radja Katia 
Published: February 2013

The phenomenon of street children could have gender‐specific aspects. The cultural hypothesis emphasises the fact that the social roles assigned to boys and girls lead to gender‐based differences in the way that the process of migration onto the street is viewed. The validity of this hypothesis is tested using data on street children in Mauritania. Our study analyses the family background of the children. The results support this hypothesis. Copyright © 2013 John Wiley & Sons, Ltd.‘The phenomenon of street children could have gender‐specific aspects’Key Practitioner Message:Studies on street children do not usually provide comparison between boys and girls despite gender specificities being important.Differences in family structure have been correlated to child gender. For example, parental absence.No significant difference emerged in terms of physical violence between the two groups in the typology.
Behavioural and Emotional Profiles of Romanian Foster Children: A Brief Report
Authors: Rus Adrian V. , Ito‐Jäger Sachiyo , Parris Sheri R. , Cross David , Purvis Karyn , Drăghici Simona 
Published: February 2013

Key Practitioner Messages:Romanian teachers tend to report more problem behaviours than foster parents.Foster children scored better than norms on some behaviour scales (girls had fewer internalising problems; boys had fewer attention problems).Scores that were worse than norms may be linked to their previous institutionalisation.Scores that were better than norms may indicate that foster care in Romania may provide living conditions that are equal to or better than conditions for children living with biological families.
Luminescent Conjugated Oligothiophenes for Sensitive Fluorescent Assignment of Protein Inclusion Bodies
Authors: Klingstedt Therése , Blechschmidt Cristiane , Nogalska Anna , Prokop Stefan , Häggqvist Bo , Danielsson Olof , Engel W. King , Askanas Valerie , Heppner Frank L. , Nilsson K. Peter R. 
Published: March 2013

AbstractSmall hydrophobic ligands identifying intracellular protein deposits are of great interest, as protein inclusion bodies are the pathological hallmark of several degenerative diseases. Here we report that fluorescent amyloid ligands, termed luminescent conjugated oligothiophenes (LCOs), rapidly and with high sensitivity detect protein inclusion bodies in skeletal muscle tissue from patients with sporadic inclusion body myositis (s‐IBM). LCOs having a conjugated backbone of at least five thiophene units emitted strong fluorescence upon binding, and showed co‐localization with proteins reported to accumulate in s‐IBM protein inclusion bodies. Compared with conventional amyloid ligands, LCOs identified a larger fraction of immunopositive inclusion bodies. When the conjugated thiophene backbone was extended with terminal carboxyl groups, the LCO revealed striking spectral differences between distinct protein inclusion bodies. We conclude that 1) LCOs are sensitive, rapid and powerful tools for identifying protein inclusion bodies and 2) LCOs identify a wider range of protein inclusion bodies than conventional amyloid ligands.