bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatoma-derived growth factor and nucleolin exist in the same ribonucleoprotein complex
Authors: Bremer Stephanie , Klein Katharina , Sedlmaier Angela , Abouzied Mekky , Gieselmann Volkmar , Franken Sebastian 
Published: January 2013

Abstract

Background
Hepatoma-derived growth factor (HDGF) is a protein which is highly expressed in a variety of tumours. HDGF has mitogenic, angiogenic, neurotrophic and antiapoptotic activity but the molecular mechanisms by which it exerts these activities are largely unknown nor has its biological function in tumours been elucidated. Mass spectrometry was performed to analyse the HDGFStrep-tag interactome. By Pull–down-experiments using different protein and nucleic acid constructs the interaction of HDGF and nucleolin was investigated further.

Results
A number of HDGFStrep-tag copurifying proteins were identified which interact with RNA or are involved in the cellular DNA repair machinery. The most abundant protein, however, copurifying with HDGF in this approach was nucleolin. Therefore we focus on the characterization of the interaction of HDGF and nucleolin in this study. We show that expression of a cytosolic variant of HDGF causes a redistribution of nucleolin into the cytoplasm. Furthermore, formation of HDGF/nucleolin complexes depends on bcl-2 mRNA. Overexpression of full length bcl-2 mRNA increases the number of HDGF/nucleolin complexes whereas expression of only the bcl-2 coding sequence abolishes interaction completely. Further examination reveals that the coding sequence of bcl-2 mRNA together with either the 5′ or 3′ UTR is sufficient for formation of HDGF/nucleolin complexes. When bcl-2 coding sequence within the full length cDNA is replaced by a sequence coding for secretory alkaline phosphatase complex formation is not enhanced.

Conclusion
The results provide evidence for the existence of HDGF and nucleolin containing nucleoprotein complexes which formation depends on the presence of specific mRNAs. The nature of these RNAs and other components of the complexes should be investigated in future.
Association of cardiovascular response to an acute resistance training session with the ACE gene polymorphism in sedentary women: a randomized trial
Authors: de Souza Jéssica Cardoso , Tibana Ramires Alsamir , de Sousa Nuno Manuel Frade , de Souza Vinícius Carolino , Karnikowski Margô G O , Prestes Jonato , Campbell Carmen Silvia Grubert 
Published: January 2013

Abstract

Background
The aim of the present study was to verify the effects of an acute resistance training (RT) session and insertion/deletion (I/D) polymorphism of the angiotensin-converting enzyme (ACE) on systolic (SBP), diastolic (DBP) and mean blood pressure (MBP), and heart rate (HR).

Methods
The sample consisted of 27 sedentary women (33.3 ± 8.2 yrs; 69.1 ± 13.8 kg; 1.57 ± 0.05 m; 27.6 ± 5.1 kg/m2) divided into two groups according to their polymorphism I/D (DD = 9; II + ID = 18). Volunteers underwent two experimental sessions: RT – an acute session performed with three sets at 60% of one-repetition maximum (1RM) interspersed with 1 minute rest interval between exercises and sets, and a control session (CON) in which they remained seated for 30 minutes in the laboratory. SBP, DBP, MBP and HR were measured before exercise and during one hour every 10 minutes after sessions, in the seated position. A two-way ANOVA for repeated measures with Tukey’s post hoc test was used for the intra and inter-group comparisons.

Results
There were no statistically significant differences on SBP, DBP and MBP after the experimental protocols, and no effect of ACE polymorphism (P > 0.05). However, comparing CON versus exercise effect size values (ES), homozygotic carriers of the allele D presented a drop in SBP which was considered moderate, while in allele I carriers it was small, 30 minutes after exercise. In MBP, homozygotic D carriers exhibited a large ES 20 minutes post-exercise. HR was higher at 10, 20 and 30 minutes after exercise as compared to pre-exercise only for carriers of the I allele (P < 0.05).

Conclusions
Therefore, an acute RT session reduces clinical BP. In addition to this; it seems that ACE polymorphism had some influence on cardiovascular response to exercise.

Trial Registration
RBR-6GDYVZ
Impact of plain packaging of tobacco products on smoking in adults and children: an elicitation of international experts’ estimates
Authors: Pechey Rachel , Spiegelhalter David , Marteau Theresa M 
Published: January 2013

Abstract

Background
Governments sometimes face important decisions in the absence of direct evidence. In these cases, expert elicitation methods can be used to quantify uncertainty. We report the results of an expert elicitation study regarding the likely impact on smoking rates in adults and children of plain packaging of tobacco products.

Methods
Thirty-three tobacco control experts were recruited from the UK (n = 14), Australasia (n = 12) and North America (n = 7). Experts’ estimates were individually elicited via telephone interviews, and then linearly pooled. Elicited estimates consisted of (1) the most likely, (2) the highest possible, and (3) the lowest possible value for the percentage of (a) adult smokers and (b) children trying smoking, two years after the introduction of plain packaging (all other things being constant) in a target country in the expert’s region of residence.

Results
The median estimate for the impact on adult smoking prevalence was a 1 percentage point decline (99% range 2.25 to 0), and for the percentage of children trying smoking was a 3 percentage point decline (99% range 6.1 to 0), the latter estimated impact being larger than the former (P < 0.001, sign test). There were no differences in either estimate by region (I2: Adults: 0; Children: 0) but there was considerable variability between experts’ estimates within regions (I2: Adults: 0.91; Children: 0.89).

Conclusions
In the absence of direct evidence for the impact of introducing plain packaging on smoking rates in adults and children, this study shows that tobacco control experts felt the most likely outcomes would be a reduction in smoking prevalence in adults, and a greater reduction in the numbers of children trying smoking, although there was substantial variability in the estimated size of these impacts. No experts judged an increase in smoking as a likely outcome.
Hydrogen-driven asymmetric reduction of hydroxyacetone to (R)-1,2-propanediol by Ralstonia eutropha transformant expressing alcohol dehydrogenase from Kluyveromyces lactis
Authors: Oda Takahiro , Oda Koji , Yamamoto Hiroaki , Matsuyama Akinobu , Ishii Masaharu , Igarashi Yasuo , Nishihara Hirofumi 
Published: January 2013

Abstract

Background
Conversion of industrial processes to more nature-friendly modes is a crucial subject for achieving sustainable development. Utilization of hydrogen-oxidation reactions by hydrogenase as a driving force of bioprocess reaction can be an environmentally ideal method because the reaction creates no pollutants. We expressed NAD-dependent alcohol dehydrogenase from Kluyveromyces lactis in a hydrogen-oxidizing bacterium: Ralstonia eutropha. This is the first report of hydrogen-driven in vivo coupling reaction of the alcohol dehydrogenase and indigenous soluble NAD-reducing hydrogenase. Asymmetric reduction of hydroxyacetone to (R)-1,2-propanediol, which is a commercial building block for antibacterial agents, was performed using the transformant as the microbial cell catalyst.

Results
The two enzymes coupled in vitro in vials without a marked decrease of reactivity during the 20 hr reaction because of the hydrogenase reaction, which generates no by-product that affects enzymes. Alcohol dehydrogenase was expressed functionally in R. eutropha in an activity level equivalent to that of indigenous NAD-reducing hydrogenase under the hydrogenase promoter. The hydrogen-driven in vivo coupling reaction proceeded only by the transformant cell without exogenous addition of a cofactor. The decrease of reaction velocity at higher concentration of hydroxyacetone was markedly reduced by application of an in vivo coupling system. Production of (R)-1,2-propanediol (99.8% e.e.) reached 67.7 g/l in 76 hr with almost a constant rate using a jar fermenter. The reaction velocity under 10% PH2 was almost equivalent to that under 100% hydrogen, indicating the availability of crude hydrogen gas from various sources. The in vivo coupling system enabled cell-recycling as catalysts.

Conclusions
Asymmetric reduction of hydroxyacetone by a coupling reaction of the two enzymes continued in both in vitro and in vivo systems in the presence of hydrogen. The in vivo reaction system using R. eutropha transformant expressing heterologous alcohol dehydrogenase showed advantages for practical usage relative to the in vitro coupling system. The results suggest a hopeful perspective of the hydrogen-driven bioprocess as an environmentally outstanding method to achieve industrial green innovation. Hydrogen-oxidizing bacteria can be useful hosts for the development of hydrogen-driven microbial cell factories.
Interactions of miR-323/miR-326/miR-329 and miR-130a/miR-155/miR-210 as prognostic indicators for clinical outcome of glioblastoma patients
Authors: Qiu Shuwei , Lin Sheng , Hu Dan , Feng Yimin , Tan Yang , Peng Ying 
Published: January 2013

Abstract

Background
Glioblastoma multiforme (GBM) is the most common and aggressive brain tumor with poor clinical outcome. Identification and development of new markers could be beneficial for the diagnosis and prognosis of GBM patients. Deregulation of microRNAs (miRNAs or miRs) is involved in GBM. Therefore, we attempted to identify and develop specific miRNAs as prognostic and predictive markers for GBM patient survival.

Methods
Expression profiles of miRNAs and genes and the corresponding clinical information of 480 GBM samples from The Cancer Genome Atlas (TCGA) dataset were downloaded and interested miRNAs were identified. Patients’ overall survival (OS) and progression-free survival (PFS) associated with interested miRNAs and miRNA-interactions were performed by Kaplan-Meier survival analysis. The impacts of miRNA expressions and miRNA-interactions on survival were evaluated by Cox proportional hazard regression model. Biological processes and network of putative and validated targets of miRNAs were analyzed by bioinformatics.

Results
In this study, 6 interested miRNAs were identified. Survival analysis showed that high levels of miR-326/miR-130a and low levels of miR-323/miR-329/miR-155/miR-210 were significantly associated with long OS of GBM patients, and also showed that high miR-326/miR-130a and low miR-155/miR-210 were related with extended PFS. Moreover, miRNA-323 and miRNA-329 were found to be increased in patients with no-recurrence or long time to progression (TTP). More notably, our analysis revealed miRNA-interactions were more specific and accurate to discriminate and predict OS and PFS. This interaction stratified OS and PFS related with different miRNA levels more detailed, and could obtain longer span of mean survival in comparison to that of one single miRNA. Moreover, miR-326, miR-130a, miR-155, miR-210 and 4 miRNA-interactions were confirmed for the first time as independent predictors for survival by Cox regression model together with clinicopathological factors: Age, Gender and Recurrence. Plus, the availability and rationality of the miRNA-interaction as predictors for survival were further supported by analysis of network, biological processes, KEGG pathway and correlation analysis with gene markers.

Conclusions
Our results demonstrates that miR-326, miR-130a, miR-155, miR-210 and the 4 miRNA-interactions could serve as prognostic and predictive markers for survival of GBM patients, suggesting a potential application in improvement of prognostic tools and treatments.
Postnatally-transmitted HIV-1 Envelope variants have similar neutralization-sensitivity and function to that of nontransmitted breast milk variants
Authors: Fouda Genevieve G , Mahlokozera Tatenda , Salazar-Gonzalez Jesus F , Salazar Maria G , Learn Gerald , Kumar Surender B , Dennison S Moses , Russell Elizabeth , Rizzolo Katherine , Jaeger Frederick , Cai Fangping , Vandergrift Nathan A , Gao Feng , Hahn Beatrice , Shaw George M , Ochsenbauer Christina , Swanstrom Ronald , Meshnick Steve , Mwapasa Victor , Kalilani Linda , Fiscus Susan , Montefiori David , Haynes Barton , Kwiek Jesse , Alam S Munir , Permar Sallie R 
Published: January 2013

Abstract

Background
Breastfeeding is a leading cause of infant HIV-1 infection in the developing world, yet only a minority of infants exposed to HIV-1 via breastfeeding become infected. As a genetic bottleneck severely restricts the number of postnatally-transmitted variants, genetic or phenotypic properties of the virus Envelope (Env) could be important for the establishment of infant infection. We examined the efficiency of virologic functions required for initiation of infection in the gastrointestinal tract and the neutralization sensitivity of HIV-1 Env variants isolated from milk of three postnatally-transmitting mothers (n=13 viruses), five clinically-matched nontransmitting mothers (n=16 viruses), and seven postnatally-infected infants (n = 7 postnatally-transmitted/founder (T/F) viruses).

Results
There was no difference in the efficiency of epithelial cell interactions between Env virus variants from the breast milk of transmitting and nontransmitting mothers. Moreover, there was similar efficiency of DC-mediated trans-infection, CCR5-usage, target cell fusion, and infectivity between HIV-1 Env-pseudoviruses from nontransmitting mothers and postnatal T/F viruses. Milk Env-pseudoviruses were generally sensitive to neutralization by autologous maternal plasma and resistant to breast milk neutralization. Infant T/F Env-pseudoviruses were equally sensitive to neutralization by broadly-neutralizing monoclonal and polyclonal antibodies as compared to nontransmitted breast milk Env variants.

Conclusion
Postnatally-T/F Env variants do not appear to possess a superior ability to interact with and cross a mucosal barrier or an exceptional resistance to neutralization that define their capability to initiate infection across the infant gastrointestinal tract in the setting of preexisting maternal antibodies.
No association between XMRV or related gammaretroviruses in Australian prostate cancer patients
Authors: Rezaei Simin D , Hearps Anna C , Mills John , Pedersen John , Tachedjian Gilda 
Published: January 2013

Abstract

Background
Xenotropic murine leukemia virus-related virus (XMRV) is a gammaretrovirus reported to be associated with prostate cancer (PC) and chronic fatigue syndrome (CFS). While the association of XMRV with CFS and PC has recently been discredited, no studies have been performed in Australian patients to investigate the association between PC and XMRV or related murine leukemia virus (MLV) in matched PC and normal tissue.

Methods
Genomic DNA (gDNA) was purified from matched normal and cancer formalin-fixed paraffin-embedded (FFPE) prostate tissue from 35 Australian PC patients with Gleason scores ranging from 7 – 10. The presence of the ribonuclease L (RNase L) polymorphism R462Q was determined by allele specific PCR. Samples were screened for XMRV and related murine leukemia virus (MLV) variants by qPCR. Contaminating mouse DNA was detected using qPCR targeting mouse intracisternal A particle long terminal repeat DNA.

Results
gDNA was successfully purified from 94% (66/70) of normal and cancer FFPE prostate tissues. RNase L typing revealed 8% were homozygous (QQ), 60% were heterozygous (RQ) and 32% were wild-type (RR) for the RNase L mutation. None of the 66 samples tested were positive for XMRV or related MLV sequences using broad MLV or XMRV specific primers with detection sensitivities of 1 viral copy of MLV/XMRV and XMRV DNA, respectively.

Conclusions
Using highly sensitive qPCR we found no evidence of XMRV or related gammaretroviruses in prostate tissues from 35 Australian PC patients. Our findings are consistent with other studies demonstrating that XMRV is a laboratory contaminant that has no role in the aetiology of PC.
Bovine papillomavirus E5 and E7 oncoproteins in naturally occurring tumors: are two better than one?
Authors: Corteggio Annunziata , Altamura Gennaro , Roperto Franco , Borzacchiello Giuseppe 
Published: January 2013

Abstract
Bovine papillomaviruses (BPVs) are oncogenic DNA viruses, which mainly induce benign lesions of cutaneous and/or mucosal epithelia in cattle. Thirteen (BPV 1–13) different viral genotypes have been characterized so far. BPVs are usually species-specific but BPV 1/2 may also infect equids as well as buffaloes and bison and cause tumors in these species. BPV-induced benign lesions usually regress, however occasionally they develop into cancer particularly in the presence of environmental carcinogenic co-factors. The major transforming protein of BPV is E5, a very short hydrophobic, transmembrane protein with many oncogenic activities. E5 contributes to cell transformation through the activation of the cellular β receptor for the platelet-derived growth factor (PDGFβ-r), it also decreases cell surface expression of major histocompatibility complex class I (MHCI) causing viral escape from immunosurveillance, and plays a role in the inhibition of the intracellular communication by means of aberrant connexin expression. E7 is considered as a weak transforming gene, it synergies with E5 in cell transformation during cancer development. E7 expression correlates in vivo with the over-expression of β1-integrin, which plays a role in the regulation of keratinocytes proliferation and differentiation. Additionally, E7 is involved in cell-mediated immune responses leading to tumour rejection, in anoikis process by direct binding to p600, and in invasion process by upregulation of Matrix metalloproteinase1 (MMP-1) expression. Studies on the role of BPV E5 and E7 oncoproteins in naturally occurring tumours are of scientific value, as they may shed new light on the biological role of these two oncogenes in cell transformation.
A Theory of Optimal Quality Reports with Inertia
Authors: Suh Jeongmeen 
Published: January 2013

Abstract
This article investigates optimal quality report design problems in a dynamic context when the service providers have moral hazard problem. I use a two-period repeated contest model between two firms in an attempt to attain a relatively better rating since a better rating leads to a bigger market share. The quality report agency calculates a firm's current rating with the inspected quality of the firm's service and carried-over advantage from the firm's past rating. The size of this advantage is the agency's control variable and models the degree of inertia in the scoring rule. The result shows that how much inertia a scoring rule is required to have, depends on several market characteristics such as the uncertainty in quality production, the consumers switching costs, and the quality investment persistency.
Determining Public Provision of Education Services in a Sequential Education Process
Authors: Romero J. Gabriel 
Published: January 2013

Abstract
This paper applies a political economy approach to the study of the endogenous determination of the size and composition of a public education budget. In this model, there are two education stages: the first is compulsory, while advanced education is optional. Parents decide on the education policy by a majority vote, children attend schools and then decide whether to get advanced education. This paper shows that even in a simple scenario where only public education is available, children's college-attendance decisions may lead the single-crossing condition to fail, which indicates that a majority voting equilibrium may not exist. In a scenario where individuals can opt for private education services, the model provides necessary and sufficient conditions for an equilibrium of the "ends-against-the-middle" type, where the poor are decisive. An implication of this result is that the opting-out feature of education may improve the welfare of the poor.
Testing for Structural Change in Heterogeneous Panels with an Application to the Euro's Trade Effect
Authors: Pauwels Laurent L. , Chan Felix , Mancini Griffoli Tommaso 
Published: January 2013

AbstractThis paper presents a structural change test for panel data models in which the break (or the change) affects some, but not all, cross-section units in the panel. The test is robust to non-normal, heteroskedastic and autocorrelated errors, as well as end-of-sample structural change. The test amounts to computing and comparing pre- and post-break sample statistics as Chow (1960) type F statistics averaged over cross-section units. The cases of known and unknown break date are both considered. Under mild assumptions, the test has a limiting standard normal distribution as the number of cross-sections tends to infinity. Monte Carlo experiments show that the test has good size and power under a wide range of circumstances, including when the break date is unknown and differs across individual units, and when errors exhibit cross-section dependence. Finally, the test is illustrated by seeking a break in the dynamics of trade among euro area countries following the introduction of the euro.
Plateau's problem for integral currents in locally non-compact metric spaces
Authors: Wenger Stefan 
Published: January 2013

Abstract.
The purpose of this article is to prove existence of mass minimizing integral currents with prescribed possibly non-compact boundary in all dual Banach spaces and furthermore in certain spaces without linear structure, such as injective metric spaces and Hadamard spaces. We furthermore prove a weak*-compactness theorem for integral currents in dual spaces of separable Banach spaces. Our theorems generalize results of Ambrosio–Kirchheim, Lang, the author, and recent results of Ambrosio–Schmidt.
Desensitization of axially asymmetric optical systems
Authors: Yabe Akira 
Published: January 2013

Abstract
Axially asymmetric optical systems treated in this paper contain both freeform surfaces and spheres. In these systems, freeform surfaces need to be allocated at the most effective position. The problem can be elegantly solved with the method of traveling freeform surfaces. The purpose of this paper is to show that the sensitivity control can be included in this design process.
Extrinsic aberrations in optical imaging systems
Authors: Sasián José 
Published: January 2013

Abstract
This paper discusses extrinsic aberrations in optical systems and provides formulas for their computation. Extrinsic aberrations result when two optical systems are combined, and there is a mismatch between the exit pupil of the first system and the entrance pupil of the second system. Some significant aberration corrections can take place as a result of extrinsic aberrations.
Sammlung Feibel: Geschichte der Kindersoftware
Authors: Feibel Thomas 
Published: January 2013

Zusammenfassung:
Der Autor und Medienexperte Thomas Feibel erzählt von den Anfängen der Kindersoftware und wie es zu der größten Kindersoftwaresammlung Deutschlands kam, die heute mit der Game Lounge der Öffentlichkeit mit über 16 000 Titeln zur Verfügung steht.
Sudden death from cardiac tamponade in an extremely low birth weight neonate with an umbilical venous catheter in situ
Authors: Gates Emma , Shaw Ben , Kokai George 
Published: January 2013

Abstract

Background: Pericardial effusion and cardiac tamponade are known to be rare but potentially fatal complications of umbilical venous catheterisation, even when the line tip is appropriately placed and is proximal to the right atrium.

Highlight of case: We report a fatal case of cardiac tamponade due to the presence of pericardial total parenteral nutrition associated with bilateral pleural effusions in an extremely low birth weight neonate with an umbilical venous catheter.

Conclusion: It is important to promptly identify and treat this rare but devastating complication of what is often considered “routine” care. Timely pericardiocentesis should be considered in a preterm baby who deteriorates suddenly and has a central venous catheter in situ.