bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gejala Menopause pada Wanita Premenopause
Authors: Fruitasari MK. Fitriani 
Published: August 2024

Latar belakang: Menopause bagi seorang wanita merupakan satu fase yang akan dicapai oleh setiap wanita pada usia 40 sampai 50 tahun. Dikatakan sudah menopause bila siklus haidnya telah berhenti sekurang-kurangnya 12 bulan. Sebelum masa menopause, ada masa persiapan menuju menopause yang disebut masa pre-menopause. Penelitian ini bertujuan menggambarkan gejala menopause pada wanita premenopause.
Metode: Desain penelitian deskriptif kuantitatif dengan jumlah sampel 96 (20% wanita premenopause) dari 482 total populasi wanita yang dipilih sesuai dengan kriteria inklusi dan eksklusi.
Hasil: Hasil penelitian ini menggambarkan bahwa gejala hot flush dirasakan oleh 10,5% wanita, gejala keringat malam dirasakan 36,5% wanita. Gejala kelelahan dan gangguan tidur dirasakan oleh 50% dan 52,1% wanita dalam skala ringan. Gejala penurunan daya ingat dirasakan oleh 46,9% wanita. Gejala rasa cemas skala ringan dirasakan 16,7% wanita. Gejala kesepian dirasakan 8,3% wanita skala ringan. Gejala depresi belum dirasakan oleh wanita.
Kesimpulan: Wanita premenenopause sudah mulai merasakan gejala menopause dalam skala ringan, seperti semburan panas (hot flash), keringat malam, kelelahan, Gangguan tidur, penurunan daya ingat, kecemasan, menyendiri dan depresi. Bahkan ada beberapa wanita premenopause tersebut yang sudah merasakan gejala menopause dalam skala menengah.
Profil Senyawa Alkaloid Dengan Metode Spektroskopi Inframerah (FTIR) Dan Penetapan Kadar Total Alkaloid Dari Ekstrak Daun Jarak Pagar (Jatropha curcas .L)
Authors: Gamah Gamah , Nastiti Kunti , Aryzki Saftia 
Published: August 2024

Latar belakang: Daun jarak pagar (Jatropha Curcas L) salah satu tanaman yang memiliki potensi khasiat obat. Secara empiris daun jarak pagar (Jatropha Curcas L) digunakan oleh Masyarakat Kalimantan Selatan untuk memperlancar proses persalinan. Salah satu kandungan senyawa yang memiliki khasiat farmakologi pada daun jarak pagar yaitu alkaloid.
Tujuan: Mengetahui senyawa alkaloid menggunakan spektroskopi inframerah (FTIR) dan menenutakan kadar alkaloid total ekstrak daun jarak (Jatropha Curcas L).
Metode: Penelitian ini menggunakan analisis deskriptif kualitatif untuk mengidentifikasi gugus fungsi alkaloid dari hasil FTIR dan analisis kuantitaitf untuk menentukan kadar alkaloid total.
Hasil: Hasil uji pendahuluan menggunakan kromatografi lapis tipis pada daun jarak pagar (Jatropha Curcas L) positif mengandung senyawa alkaloid. Hasil FTIR menunjukan bahwa terdapat kandungan senyawa alkaloid dengan adanya gugus fungsi yang diperoleh C-H, C=O, C-O, N-H dan C-H aromatis yang merupakan karakteristik senyawa alkaloid dan kadar alkaloid total 5,645% (g/5g).
Kesimpulan: Ekstrak daun jarak pagar ( Jatropha Curcas L) Menggunakan analisis FTIR menunjukan kandungan senyawa alkaloid dan kadar alkaloid total diperoleh sebesar 5,645% g/5g).
Identifying the plasma metabolome responsible for mediating immune cell action in severe COVID-19: a Mendelian randomization investigation
Authors: Zhang Yixia , Hua Jie , Chen Liang 
Published: August 2024

IntroductionThe immune response regulates the severity of COVID-19 (sCOVID-19). This study examined the cause-and-effect relationship between immune cell traits (ICTs) and the risk of severe COVID-19. Additionally, we discovered the potential role of plasma metabolome in modulating this risk.MethodsEmploying data from a genome-wide association study (GWAS), we conducted a two-sample Mendelian randomization (MR) assessment of 731 genetic ICTs and sCOVID-19 (5,101 cases, 1,383,241 controls) incidence. The MR analysis was utilized to further quantitate the degree of plasma metabolome-mediated regulation of immune traits in sCOVID-19.ResultsThe inverse variance weighted method recognized 2 plasma metabolites (PMs) responsible for casual associations between immune cells and sCOVID-19 risk. These included Tridecenedioate (C13:1-DC) which regulated the association between CD27 on IgD- CD38br (OR 0.804, 95% CI 0.699–0.925, p = 0.002) and sCOVID-19 risk (mediated proportion: 18.7%); arginine to citrulline ratio which controlled the relationship of CD39 on monocyte (OR 1.053, 95% CI 1.013–1.094, p = 0.009) with sCOVID-19 risk (mediated proportion: -7.11%). No strong evidence that genetically predicted sCOVID-19 influenced the aforementioned immune traits.ConclusionIn this study, we have successfully identified a cause-and-effect relationship between certain ICTs, PMs, and the likelihood of contracting severe COVID-19. Our findings can potentially improve the accuracy of COVID-19 prognostic evaluation and provide valuable insights into the underlying mechanisms of the disease.
In situ occurrence and conservation of Beta vulgaris subsp. maritima ((L.) Arcangeli) in Italy
Authors: Zucchini Cecilia , Raggi Lorenzo , Grassi Anna , Spataro Giorgia , Gigante Daniela , Negri Valeria 
Published: August 2024

IntroductionCrop Wild Relatives (CWR) have great socioeconomic importance for humans harbouring a broad spectrum of diversity and being important elements of different habitats. Beta vulgaris subsp. maritima ((L.) Arcangeli), also known as sea beet, is an important CWR of cultivated beets (GP-1). The high adaptability of this taxon to different environmental conditions, as well as its tolerance/resistance to different biotic and abiotic stresses, makes it a vital source for sugar beet improvement. Aim of this work was to analyse the in situ and ex situ status of sea beet population conservation in Italy, so as to guide protection activities and new collecting missions.MethodsGeographical distribution data of populations were that occur in the wild and conserved in genebanks were assembled from different databases and submitted to data quality control. Distribution, habitat characterization, land cover and use of the involved sites were then evaluated to provide insight into the current condition of areas hosting this CWR diversity. The presence of populations within vs. outside Italian protected areas was also evaluated. A density analysis of the records was performed and the adequateness of sea beet ex situ conservation, in terms of number of conserved accessions, was finally estimated.ResultsA collection of 138 B. vulgaris subsp. maritima high quality georeferenced records were obtained, mainly distributed into Mediterranean Biogeographic Region. About 22% of the considered populations occurs in protected sites of the Natura 2000 Network, while about 15% in areas recorded in the Official list of protected natural areas (EUAP). Occurrences within protected areas are founded primarily in natural environments, whereas those outside are mainly located in urban and cultivated areas. The comparison of distribution and density analysis results revealed the presence of several gaps between sites hosting in situ populations and sites where ex situ conserved accessions were originally collected.DiscussionHere presented data indicates that the protection status for sea beet in Italy can be considered only partially adequate; more proactive protection measures should be foreseen to increase the role of protected areas in safeguarding in situ conservation. “Out of reserve management” for populations outside protected areas should also be developed, as well as new collecting missions carried out.
Marker-trait association analysis for easy fruit destemming and mechanical harvestability traits in New Mexican chile pepper (Capsicum annuum L.)
Authors: Khokhar Ehtisham S. , Lozada Dennis Nicuh , Ali Mohsin , Khan Muhammad Ibrar , Nourbakhsh Seyed Shahabeddin , Walker Stephanie 
Published: August 2024

IntroductionChile pepper (Capsicum annuum L.) mechanization is a promising alternative to traditional hand harvesting due to the costs associated with manual harvest, as well as the increasing unavailability of skilled manual chile harvesters. This study aimed to identify single nucleotide polymorphisms (SNPs) associated with mechanical harvestability (MH) and yield-related traits using multi-locus genome-wide association mapping approaches in a C. annuum association mapping population.MethodsA C. annuum association mapping panel for mechanical harvest was manually direct seeded in an augmented block design in two locations. After filtration, imputation, and quality control 27,291 single nucleotide polymorphism (SNP) markers were used for association analyses. Six multi-locus GWAS models were implemented to identify marker trait association.Results and DiscussionMulti-locus GWAS models identified 12 major SNP markers (R2 &gt; 10) across nine chromosomes associated with plant architecture, easy destemming traits, and yield parameters. The presence of a major QTL in chromosome P2, dstem2.1, identified recently to be associated with destemming force, was confirmed. Mature green and mature red yield shared three SNP markers mapped on chromosome P3, P5, and P6 explaining 11.94% to 25.15% of the phenotypic variation. Candidate gene analysis for the significant loci identified 19 candidate genes regulating different phytohormone biosynthesis/signaling, metabolic processes, transcription, methylation, DNA repair/replication, and RNA splicing, with potential roles in controlling plant architecture and morphology. The diverse positions of the associated SNPs suggest the complex nature of these quantitative traits, involvement of multiple genetic factors, and novel significant marker-trait associations. Results from this study will be relevant for genetic improvement of mechanical harvestability traits in New Mexican chile pepper using molecular markerassisted breeding and selection.
Advancing stroke diagnosis and management through nuclear medicine: a systematic review of clinical trials
Authors: Azhari Hala F. 
Published: August 2024

IntroductionDespite advancements in stroke care, challenges persist in timely triage and treatment initiation to prevent the burden of stroke-related disabilities. Although nuclear medicine has shown promise, no imaging technique has yet provided a sufficiently rapid, precise, and cost-effective approach to routine stroke management. This study aims to review the clinical application of nuclear medicine in stroke diagnosis and treatment.MethodsA systematic search of the Cochrane, EU Clinical Trials Register, ISRCTN, the International Stroke Trial, and the ClinicalTrials.gov database was conducted to find all registered trials reporting nuclear medicine’s clinical applications in stroke up to June 07, 2024.ResultsAmong the 220 screened trials, 51 (36 interventional; 15 observational) met the eligibility criteria. Participants were older than 18 years old, with only six studies including pediatric under 17 years old, with a total of 11,262 stroke (9,232 ischemic; 2,030 haemorrhagic) participants. The bias risk varied across trials but remained mostly low to moderate.DiscussionThe review highlighted nuclear medicine’s significant contributions to stroke diagnosis and management, notably through mobile stroke units, pre-hospital acute stroke magnetic resonance image (MRI) based biomarkers, and MRI-based stroke mechanisms for 4D flow nuclear imaging. These advancements have generally reduced treatment delays and enhance clinical outcomes post-stroke. Specifically, radiopharmaceutical radiotracers can effectively discriminate between strokes and mimics, particularly in high-risk patients. Integrating novel positron emission tomography (PET) radiotracer 18F glycoprotein 1 and radionuclide angiography may improve sensitivity and specificity in thrombi detection for decisions regarding stenting or carotid endarterectomy, and the single-photon emission computed tomography and PET integration with ferumoxytol radiotracer-enhanced MRI enables functional imaging for evaluating cerebral perfusion, metabolic activity, and neuroinflammatory markers post-stroke. Overall, the integration of nuclear medicine into multimodal imaging equipment like computed-tomography PET and MRI-PET offers a more comprehensive picture of the brain. Nevertheless, further research is needed on novel stroke imaging techniques and standardization across stroke centers for optimal performance.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024541680, identifier PROSPERO CRD(42024541680).
Development of a comprehensive risk prediction model for arterial stiffness assessment in individuals with obesity
Authors: Pescari Denisa , Borlea Andreea , Mihuta Simina , Stoian Dana 
Published: August 2024

IntroductionObesity in adults is a known risk factor for cardiovascular events and is associated with a decline in arterial elasticity. This study aims to evaluate the utility of pulse wave analysis (PWA) parameters in routine clinical practice for the primary prevention of cardiovascular events by developing a prediction model for arterial stiffness among obese and overweight individuals.MethodsThe study enrolled 84 adult patients, aged 18 to 85 years, with varying degrees of weight status, including optimal weight, overweight, and obesity. The lifestyle habits, the personal and family history of cardiometabolic diseases, as well the clinical evaluation that included BMI (body mass index), WHR (waist-to-hip ratio), WC (waist circumferance) were performed. PWA evaluation was conducted using the Mobil-O-Graph device, assessing the following parameters: pulse wave velocity (PWV), augmentation index (AIx), heart rate (HR), central pulse pressure (cPP), peripheral and central blood pressure (SBP, DBP, cSBP, cDBP). Body composition analysis was performed using the TANITA BC-418 body analyzer. Laboratory results from the past 3 months were also collected during initial nutritional consultations for each patient.ResultsFamily history of cardiovascular events showed positive correlations with all PWA parameters, while diabetes history only with PWV and family history of obesity with PWV, DBP, and cSBP. Insufficient sleep duration showed positive associations with all arterial stiffness parameters except cDBP. Smoking status correlated with significantly elevated PWV and Aix values, while insufficient physical activity was associated solely with PWV. Positive correlations were showed between current weight and PWV, while WC demonstrated positive associations with PWV, SBP, and cSBP. Body composition analysis revealed significant associations between trunk adipose tissue mass (%) and PWV, SBP, and cSBP. Hydration status (%) emerged as an independent predictor for PWV, exhibiting an inverse relationship. HOMA-IR (Homeostatic Model Assessment for Insulin Resistance) showed a strong positive correlation with PWV. Negative associations were observed with HDL-c and vitamin D. Threshold values for age, cDBP and Cardiac Index providing positive diagnostic for vascular impairment.ConclusionThe assessment of arterial stiffness can be considered a reliable approach to prevent obesity-related cardiovascular events and facilitate the comprehensive management of such pathologies.
Cerebrospinal fluid characteristics of patients presenting for evaluation of pediatric acute-neuropsychiatric syndrome
Authors: Pooni Rajdeep , Zheng Wynne , Ma Meiqian , Silverman Melissa , Xie Yuhuan , Farhadian Bahare , Thienemann Margo , Mellins Elizabeth , Frankovich Jennifer 
Published: August 2024

ObjectivesThis study characterizes cerebral spinal fluid (CSF) indices including total protein, the albumin quotient, IgG index and oligoclonal bands in patients followed at a single center for pediatric acute-neuropsychiatric syndrome (PANS) and other psychiatric/behavioral deteriorations.MethodsIn a retrospective chart review of 471 consecutive subjects evaluated for PANS at a single center, navigational keyword search of the electronic medical record was used to identify patients who underwent lumbar puncture (LP) as part of the evaluation of a severe or atypical psychiatric deterioration. Psychiatric symptom data was ascertained from parent questionnaires and clinical psychiatric evaluations. Inclusion criteria required that subjects presented with psychiatric deterioration at the time of first clinical visit and had a lumbar puncture completed as part of their evaluation. Subjects were categorized into three subgroups based on diagnosis: PANS (acute-onset of severe obsessive compulsive disorder (OCD) and/or eating restriction plus two other neuropsychiatric symptoms), autoimmune encephalitis (AE), and “other neuropsychiatric deterioration” (subacute onset of severe OCD, eating restriction, behavioral regression, psychosis, etc; not meeting criteria for PANS or AE).Results71/471 (15.0 %) of patients underwent LP. At least one CSF abnormality was seen in 29% of patients with PANS, 45% of patients with “other neuropsychiatric deterioration”, and 40% of patients who met criteria for autoimmune encephalitis. The most common findings included elevated CSF protein and/or albumin quotient. Elevated IgG index and IgG oligoclonal bands were rare in all three groups.ConclusionElevation of CSF protein and albumin quotient were found in pediatric patients undergoing LP for evaluation of severe psychiatric deteriorations (PANS, AE, and other neuropsychiatric deteriorations). Further studies are warranted to investigate blood brain barrier integrity at the onset of the neuropsychiatric deterioration and explore inflammatory mechanisms.
Prenatal opioid exposure significantly impacts placental protein kinase C (PKC) and drug transporters, leading to drug resistance and neonatal opioid withdrawal syndrome
Authors: Radhakrishna Uppala , Radhakrishnan Rupa , Uppala Lavanya V. , Muvvala Srinivas B. , Prajapati Jignesh , Rawal Rakesh M. , Bahado-Singh Ray O. , Sadhasivam Senthilkumar 
Published: August 2024

BackgroundNeonatal Opioid Withdrawal Syndrome (NOWS) is a consequence of in-utero exposure to prenatal maternal opioids, resulting in the manifestation of symptoms like irritability, feeding problems, tremors, and withdrawal signs. Opioid use disorder (OUD) during pregnancy can profoundly impact both mother and fetus, disrupting fetal brain neurotransmission and potentially leading to long-term neurological, behavioral, and vision issues, and increased infant mortality. Drug resistance complicates OUD and NOWS treatment, with protein kinase regulation of drug transporters not fully understood.MethodsDNA methylation levels of ATP-binding cassette (ABC) and solute carrier (SLC) drug transporters, along with protein kinase C (PKC) genes, were assessed in 96 placental samples using the Illumina Infinium MethylationEPIC array (850K). Samples were collected from three distinct groups: 32 mothers with infants prenatally exposed to opioids who needed pharmacological intervention for NOWS, 32 mothers with prenatally opioid-exposed infants who did not necessitate NOWS treatment, and 32 mothers who were not exposed to opioids during pregnancy.ResultsWe identified 69 significantly differentially methylated SLCs, with 24 hypermethylated and 34 hypomethylated, and 11 exhibiting both types of methylation changes including SLC13A3, SLC15A2, SLC16A11, SLC16A3, SLC19A2, and SLC26A1. We identified methylation changes in 11 ABC drug transporters (ABCA1, ABCA12, ABCA2, ABCB10, ABCB5, ABCC12, ABCC2, ABCC9, ABCE1, ABCC7, ABCB3): 3 showed hypermethylation, 3 hypomethylation, and 5 exhibited both. Additionally, 7 PKC family genes (PRKCQ, PRKAA1, PRKCA, PRKCB, PRKCH, PRKCI, and PRKCZ) showed methylation changes. These genes are associated with 13 pathways involved in NOWS, including ABC transporters, bile secretion, pancreatic secretion, insulin resistance, glutamatergic synapse, and gastric acid secretion.ConclusionWe report epigenetic changes in PKC-related regulation of drug transporters, which could improve our understanding of clinical outcomes like drug resistance, pharmacokinetics, drug-drug interactions, and drug toxicity, leading to maternal relapse and severe NOWS. Novel drugs targeting PKC pathways and transporters may improve treatment outcomes for OUD in pregnancy and NOWS.
Bioelectrical impedance phase angle and the mortality in critically ill children
Authors: Yang Jiongxian , Zhang Jie , Liu Jun , Liu Gang , Qian Suyun 
Published: August 2024

BackgroundPhase angle (PhA) is a variable obtained from bioelectrical impedance analysis (BIA). It is highly sensitive and specific and is commonly used in clinical nutrition assessment. Recently, PhA has shown promise in predicting clinical outcomes, especially as a new indicator of mortality, but its use in pediatric research is limited. This study aims to investigate the association between PhA measured at admission using BIA and PICU length of stay (LOS) and 60-day mortality in critically ill children and adolescents.MethodsA consecutive series of pediatric patients in the PICU underwent BIA measurements within 72 h of admission. All patients met the inclusion and exclusion criteria. Patient demographics, anthropometric measurements, pediatric index of mortality 2 score (PIM-2), and laboratory exams were recorded. Kaplan–Meier (K–M) survival curves were constructed based on the critical PhA value to assess differences in survival status within the 60-day window. Multivariate cox regression model was employed to illustrate the relationship between PhA and 60-day mortality rates. The Youden’s index method was used to identify the critical cut-off value for PhA in relation to mortality rates. ROC curves provided the area under the curve (AUC) and a 95% confidence interval (CI).ResultsA total of 205 pediatric patients (118 boys) were included, with a mean age of 9.2 years (±6.0). Survival curves indicated a cutoff value of 3.1°, with higher survival in patients with PhA ≥3.1° compared to those with PhA &lt;3.1° (F = 10.51, p &lt; 0.0001). The area under the ROC curve was 0.70, with a sensitivity of 0.65 and specificity of 0.72. Total hospital LOS was longer in the PhA &lt;3.1° group compared to the PhA ≥3.1° group (p = 0.000). The PhA &lt;3.1° group had a longer PICU LOS (adjusted for age and sex, HR 1.871, p = 0.000, log-rank test, p = 0.000). PhA and PIM-2 were two independently significant correlated variables (p &lt; 0.05) for the 60-day mortality rate in this study.ConclusionLow PhA in patients is associated with longer PICU LOS and an increased risk of PICU patient mortality. PhA not only serves as an indicator for monitoring pediatric nutrition but also as a prognostic indicator for PICU patients.