bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring molecular changes in the management of myelodysplastic syndromes
Authors: Duployez Nicolas , Preudhomme Claude 
Published: June 2024

SummaryThe ongoing or anticipated therapeutic advances as well as previous experience in other malignancies, including acute myeloid leukaemia, have made molecular monitoring a potential interesting tool for predicting outcomes and demonstrating treatment efficacy in patients with myelodysplastic syndromes (MDS). The important genetic heterogeneity in MDS has made challenging the establishment of recommendations. In this context, high‐throughput/next‐generation sequencing (NGS) has emerged as an attractive tool, especially in patients with high‐risk diseases. However, its implementation in clinical practice still suffers from a lack of standardization in terms of sensitivity, bioinformatics and result interpretation. Data from literature, mostly gleaned from retrospective cohorts, show NGS monitoring when used appropriately could help clinicians to guide therapy, detect early relapse and predict disease evolution. Translating these observations into personalized patient management requires a prospective evaluation in clinical research and remains a major challenge for the next years.
Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure
Authors: Suzuki Makoto , Yasue Naoko , Ueno Naoto 
Published: June 2024

AbstractDuring the formation of the neural tube, the primordium of the vertebrate central nervous system, the actomyosin activity of cells in different regions drives neural plate bending. However, how the stiffness of the neural plate and surrounding tissues is regulated and mechanically influences neural plate bending has not been elucidated. Here, we used atomic force microscopy to reveal the relationship between the stiffness of the neural plate and the mesoderm during Xenopus neural tube formation. Measurements with intact embryos revealed that the stiffness of the neural plate was consistently higher compared with the non‐neural ectoderm and that it increased in an actomyosin activity‐dependent manner during neural plate bending. Interestingly, measurements of isolated tissue explants also revealed that the relationship between the stiffness of the apical and basal sides of the neural plate was reversed during bending and that the stiffness of the mesoderm was lower than that of the basal side of the neural plate. The experimental elevation of mesoderm stiffness delayed neural plate bending, suggesting that low mesoderm stiffness mechanically supports neural tube closure. This study provides an example of mechanical interactions between tissues during large‐scale morphogenetic movements.
Vascular endothelial growth factor accelerates healing of foot ulcers in diabetic rats via promoting M2 macrophage polarization
Authors: Liu Fei , Xu Xianrui , Sun Tao 
Published: June 2024

AbstractAimThe objective was to investigate the specific role and the regulatory mechanism of vascular endothelial growth factor (VEGF) during wound healing in diabetic foot ulcer (DFU).MethodsStreptozotocin‐induced diabetic rats were used to establish a DFU animal model. VEGF and Axitinib (a specific inhibitor of VEGFR) were used for treatment in vivo. The wounds at different time points were imaged and histological analysis of the wounds were performed by haematoxylin and eosin (H&E) staining and Masson's trichrome staining. Immunohistochemical staining was conducted to examine CD31 and eNOS expression in the wounds. Immunofluorescence assay and quantitative real‐time PCR were performed to examine macrophage markers. In addition, THP‐1 was differentiated to macrophages, and then treated with interleukin (IL)‐4 to induce M2 macrophages, followed by VEGF treatment. The conditional medium (CM) from VEGF‐mediated macrophages were collected to culture human dermal fibroblasts (HDFs). Cell viability and migration were measured by Cell Counting Kit (CCK)‐8, wound‐healing and Transwell assays, respectively.ResultsVEGF treatment remarkably accelerated wound healing of DFU rats. VEGF promoted collagen deposition and elevated CD31 and eNOS expression, confirming the pro‐angiogenesis of VEGF around diabetic wound in rats. Meanwhile, VEGF restricted pro‐inflammatory cytokines and increased F4/80 and CD206 expression, highlighting the activated macrophages and enhanced M2 macrophages following VEGF treatment in diabetic wounds of DFU rats. However, Axitinib exerted an opposite function to VEGF in DFU rats. Moreover, VEGF directly promoted macrophage polarization toward M2 phenotype in vitro, and the CM from VEGF‐mediated M2 macrophages markedly promoted HDFs proliferation, migration and collagen deposition.ConclusionVEGF might accelerate the wound healing of DFU through promoting M2 macrophage polarization and fibroblast migration.
A novel framework to assess haematology and oncology registration trials: The THEOREMM project
Authors: Olivier Timothée , Haslam Alyson , Burke Patricia , Boutron Isabelle , Naudet Florian , Ioannidis John P. A. , Prasad Vinay 
Published: June 2024

AbstractBackgroundMethodological limitations affect a significant number of oncology and haematology trials, raising concerns about the applicability of their results. For example, a suboptimal control arm or limited access to best care upon progression may skew the trial results toward a benefit in the experimental arm. Beyond the fact that such limitations do not prevent drugs reaching the market, other assessment tools, such as those developed by professional societies—ESMO‐MCBS and ASCO Value Framework—do not integrate these important shortcomings.MethodsWe propose creating a novel framework with the scope of assessing registration cancer clinical trials in haematology and oncology (randomized or single arm)—that is trials leading to a marketing authorization. The main steps of the methods are (1) assembling a scientific board; (2) defining the scope, goal and methods through pre‐specified, pre‐registered and protocolized methodology; (3) preregistration of the protocol; (4) conducting a scoping review of limitations and biases affecting oncology trials and assessing existing scores or methods; (5) developing a list of features to be included and assessed within the framework; (6) assessing each feature through a questionnaire sent to highly cited haematologists and oncologists involved in clinical trials; and (7) finalizing the first version of framework.ResultsNot applicable.ConclusionsOur proposal emerged in response to the lack of consideration for key limitations in current trial assessments. The goal is to create a framework specifically designed to assess single trials leading to marketing authorization in the field of oncology and haematogy.
An integrated approach for detecting and classifying pores and surface topology for fatigue assessment 316L manufactured by powder bed fusion of metals using a laser beam using μCT and machine learning algorithms
Authors: Diller Johannes , Siebert Ludwig , Winkler Michael , Siebert Dorina , Blankenhagen Jakob , Wenzler David , Radlbeck Christina , Mensinger Martin 
Published: June 2024

AbstractThis research aims to detect and analyze critical internal and surface defects in metal components manufactured by powder bed fusion of metals using a laser beam (PBF‐LB/M). The aim is to assess their impact on the fatigue behavior. Therefore, a combination of methods, including image processing of micro‐computed tomography (
CT) scans, fatigue testing, and machine learning, was applied. A workflow was established to contribute to the nondestructive assessment of component quality and mechanical properties. Additionally, this study illustrates the application of machine learning to address a classification problem, specifically the categorization of pores into gas pores and lack of fusion pores. Although it was shown that internal defects exhibited a reduced impact on fatigue behavior compared with surface defects, it was noted that surface defects exert a higher influence on fatigue behavior. A machine learning algorithm was developed to predict the fatigue life using surface defect features as input parameters.
Self‐directed use of machine translation among language learners: Does it lead to disruptive L2 avoidance?
Authors: Murtisari Elisabet Titik , Kristianto Andreas Kukuh , Bonar Gary 
Published: June 2024

AbstractRapid improvements in the capabilities of machine translation (MT) raise questions about possible increases in overreliance on MT among lower‐proficiency or novice level language learners. This study investigated how such learners described their use of online MT for independent reading and writing tasks, and whether this included descriptions of second language (L2) avoidance behavior. We also explored learners' reasons for using MT and the perceived effects on their language learning. Findings from in‐depth interviews with eight second‐year tertiary language learners suggest that using MT could exceed desirable use among such learners in relation to the language learning objectives, resulting in language avoidance. Although MT helped them in completing language tasks, its effects were perceived to be detrimental toward their abilities to express themselves in the L2. As such, the use of MT may lead to purely superficial language learning in formal language programs. These findings suggest language educators need to consider instructional scaffolding in language programs for such learners and guidelines to assist their autonomous use of the tool.
Transpositions as a hopeful methodology for organizational studies
Authors: Martin Lydia A. , Sayers Janet G. , Carroll Brigid 
Published: June 2024

AbstractIn this paper, we introduce Braidotti’s notion of transpositions as a methodology and strategy for ‘hopeful disruptions’ in the context of organizational inquiry. Transpositions consist of six interwoven practices―(1) embracing alternative ways of seeing and understanding the world, (2) assessing and critiquing imaginary forms, (3) preparing to make the ‘creative leap’, (4) accounting for locations and positioning, (5) storying the ‘in‐between’ space, and (6) developing new frames of resonance for existing cultural formations, such as heroic leadership. Underpinned by feminist posthumanist thinking, transposition practices produce ‘generative cracks’ in hegemonic systems and in dominant social imaginaries, as well as bringing forth affirmative alternatives for thought and practice. As a feminist approach, it is also concerned with engaging gender differently and strategically to chart paths out of restrictive categories and reductive, individualist notions of being. Playing with the inherently subversive nature of this approach and tapping into our desire to disrupt the masculine ‘common sense’ of much social science research, we draw on the insights and writings of feminist speculative fiction authors to elaborate on the six practices and their implications for researchers. We further demonstrate the potential for transpositions in organizational studies through a discussion of radical empiricist approaches to inquiry and collaborative research projects.
Host plants and landscape predict use of roadside habitat by breeding monarchs
Authors: Cariveau Alison J. Banks , Haynes Grace A. , Perish Patrick , Tuerk Karen , Nootenboom Chris , Mitchell Timothy , Lonsdorf Eric , Oberhauser Karen , Snell‐Rood Emilie C. 
Published: June 2024

Abstract
Vegetation along roadside edges, while posing some risks, can also provide breeding habitat for monarch butterflies.
We investigated how landscape and site‐specific factors affect the quality of roadsides as monarch breeding habitat. We surveyed more than 250 sites along roadsides throughout Minnesota for milkweeds, floral resources and monarch eggs and larvae.
We found that milkweed distribution was affected by adjacent land use and geography, with milkweeds more likely to be present in roadsides adjacent to crop and grassland and the south and west of the state.
The presence and density of monarch eggs and larvae were primarily driven by milkweed density, with the likelihood of monarch presence at each site increasing with milkweed density. The density of monarchs per plant increased in sites with lower densities of milkweed, suggesting that monarch eggs are concentrated more densely on fewer plants where milkweed is scarce and spread more thinly across plants where milkweed is common. Monarch density per milkweed increased as sites were farther from core grasslands, to the south, and on narrower roadsides, also suggesting use may be higher in areas with a scarcity of habitat. The density of monarch eggs and larvae was higher in mowed than unmowed milkweed.
Our study suggests that roadside habitats effectively provide breeding locations for monarchs, regardless of adjacent land use type and even when distant to high‐quality natural areas. Our findings assist conservation practitioners in planning and managing roadside habitats for monarchs and other pollinators.
Successfully initiating an escalation of care in acute ward settings—A qualitative observational study
Authors: Ede J. , Kent B. , Watkinson P. , Endacott R. 
Published: June 2024

AbstractAimsTo address knowledge gaps by (i) developing a theoretical understanding of escalation and (ii) identifying escalation success factors.DesignNon‐participant observations were used to examine deteriorating patient escalation events.MethodsEscalation event data were collected by a researcher who shadowed clinical staff, between February 16th 2021 and March 17th 2022 from two National Health Service Trusts. Events were analysed using Framework Analysis. Escalation tasks were mapped using a Hierarchical Task Analysis diagram and data presented as percentages, frequency and 95% CI.ResultsA total of 38 observation sessions were conducted, totaling 105 h, during which 151 escalation events were captured. Half of these were not early warning score‐initiated and resulted from bleeding, infection, or chest pain. Four communication phenotypes were observed in the escalation events. The most common was Outcome Focused Escalation, where the referrer expected specific outcomes like blood cultures or antibiotic prescriptions. Informative Escalations were often used when a triggering patient's condition was of low clinical concern and ranked as the second most frequent escalation communication type. General Concern Escalations occurred when the referrer did not have predetermined expectations. Spontaneous Interaction Escalations were the least frequently observed, occurring opportunistically in communal workspaces.ConclusionHalf of the events were non‐triggering escalations and understanding these can inform the design of systems to support staff better to undertake them. Escalation is not homogenous and differing escalation communication phenotypes exist. Informative Escalations represent an organizational requirement to report triggering warning scores and a targeted reduction of these may be organizationally advantageous. Increasing the frequency of Spontaneous Escalations, through hospital designs, may also be beneficial.Impact StatementOur work highlights that a significant proportion of escalation workload occurs without a triggering early warning score and there is scope to better support these with designed systems. Further examination of reducing Informative and increasing Spontaneous Escalations is also warranted.Patient and Public ContributionExtensive PPIE was completed throughout the lifecycle of this study. PPIE members validated the research questions and overarching aims of the overall study. PPIE members contributed to the design of the study reviewed documents and the final data generated.
Fecal microbiota transplantation ameliorates high‐fat diet‐induced memory impairment in mice
Authors: Pereira Louise Tavares Garcia , Vilela Wembley Rodrigues , Bellozi Paula Maria Quaglio , Engel Daiane Fátima , de Paula Gabriela Cristina , de Andrade Rafael Rocha , Mortari Márcia Renata , de Melo Teixeira Marcus , Coleine Claudia , Figueiredo Cláudia Pinto , de Bem Andreza Fabro , Amato Angélica Amorim 
Published: June 2024

AbstractGut dysbiosis is linked to metabolic and neurodegenerative diseases and comprises a plausible link between high‐fat diet (HFD) and brain dysfunction. Here we show that gut microbiota modulation by either antibiotic treatment for 5 weeks or a brief 3‐day fecal microbiota transplantation (FMT) regimen from low‐fat (control) diet‐fed mice decreased weight gain, adipose tissue hypertrophy, and glucose intolerance induced by HFD in C57BL/6 male mice. Notably, gut microbiota modulation by FMT completely reversed impaired recognition memory induced by HFD, whereas modulation by antibiotics had less pronounced effect. Improvement in recognition memory by FMT was accompanied by decreased HFD‐induced astrogliosis in the hippocampal cornu ammonis region. Gut microbiome composition analysis indicated that HFD diminished microbiota diversity compared to control diet, whereas FMT partially restored the phyla diversity. Our findings reinforce the role of the gut microbiota on HFD‐induced cognitive impairment and suggest that modulating the gut microbiota may be an effective strategy to prevent metabolic and cognitive dysfunction associated with unfavorable dietary patterns.image
Cdk5 inhibition in the SOD1G93A transgenic mouse model of amyotrophic lateral sclerosis suppresses neurodegeneration and extends survival
Authors: Kim Ahwon , Lee Do‐Yeon , Sung Jung‐Joon 
Published: June 2024

AbstractDeregulated cyclin‐dependent kinase 5 (Cdk5) activity closely correlates with hyperphosphorylated tau, a common pathology found in neurodegenerative diseases. Previous postmortem studies had revealed increased Cdk5 immunoreactivity in amyotrophic lateral sclerosis (ALS); hence, we investigated the effects of Cdk5 inhibition on ALS model mice and neurons in this study. For the in vitro study, motor neuron cell lines with wild‐type superoxide dismutase 1 (SOD1) or SOD1G93A and primary neuronal cultures from SOD1G93A transgenic (TG) mice or non‐TG mice were compared for the expression of proteins involved in tau pathology, neuroinflammation, apoptosis, and neuritic outgrowth by applying Cdk5‐small interfering RNA or Cdk5‐short hairpin RNA (shRNA). For the in vivo study, SOD1G93A mice and non‐TG mice were intrathecally injected with adeno‐associated virus 9 (AAV9)‐scramble (SCR)‐shRNA or AAV9‐Cdk5‐shRNA at the age of 5 weeks. Weight and motor function were measured three times per week from 60 days of age, longevity was evaluated, and the tissues were collected from 90‐day‐old or 120‐day‐old mice. Neurons with SOD1G93A showed increased phosphorylated tau, attenuated neuritic growth, mislocalization of SOD1, and enhanced neuroinflammation and apoptosis, all of which were reversed by Cdk5 inhibition. Weights did not show significant differences among non‐TG and SOD1G93A mice with or without Cdk5 silencing. SOD1G93A mice treated with AAV9‐Cdk5‐shRNA showed significantly delayed disease onset, delayed rotarod failure, and prolonged survival compared with those treated with AAV9‐SCR‐shRNA. The brain and spinal cord of SOD1G93A mice intrathecally injected with AAV9‐Cdk5‐shRNA exhibited suppressed tau pathology, neuroinflammation, apoptosis, and an increased number of motor neurons compared to those of SOD1G93A mice injected with AAV9‐SCR‐shRNA. Cdk5 inhibition could be an important mechanism in the development of a new therapeutic strategy for ALS.image
The dual‐path mechanism underlying the influence of customers' negative feedback on the job crafting
Authors: Liu Bo , Yun Jiang , Zhao Jinjin 
Published: June 2024

AbstractAlthough previous studies have generally explored the important role played by customers' negative feedback in shaping employees' behaviour, these studies have only briefly discussed the one‐sided effects of such feedback on employees' cognition or emotions. In addition, previous studies have not discussed in depth whether customers' negative feedback has both positive and negative effects. Based on the theoretical background of the cognitive–affective processing system, we constructed a dual‐path model of the impact of customers' negative feedback on job crafting via both cognitive and affective paths and investigated the moderating effect of the error management climate in this context. A three‐stage survey design was used to examine these assumptions by referencing data collected from 382 frontline employees working in various tourism and hospitality companies. In line with our expectations, we found that customers' negative feedback can have a double‐edged sword effect via these two paths and that the error management climate moderates this dual‐path mechanism. We conclude this research by discussing the theoretical and practical implications of our findings.
Respiratory reference values for bottlenose dolphins (Tursiops spp.) and its importance for conservation
Authors: Cauture Fabien , Sweeney Jay , Stone Rae , Fahlman Andreas 
Published: June 2024

AbstractRespiratory disease is one of the main causes for morbidity and mortality in cetaceans, which highlights the importance of understanding normal lung function and how it may impede homeostasis, and diving capacity. In addition, the use of breathing frequency as a proxy for metabolic rate requires a better estimate of the normal range of tidal volume, respiratory flow, and breath durations. In the current study, we use data on clinically healthy bottlenose dolphins (Tursiops spp.) collected over a decade to define how lung function varies with body mass, age, and sex while in water or during voluntary beaching. The data show that tidal volume consistently varies with body mass both during spontaneous and forced breaths both in water and while beached. Both peak expiratory and inspiratory flow varies with body mass, but also in some circumstances with sex and age. Total and inspiratory breath durations only varied with body mass during forced breaths on land. Expired tidal volume varied with both body mass and either total or expiratory breath duration. These data provide baseline for respiratory function in healthy bottlenose dolphins and suggest that either total or expiratory breath duration provide a useful proxy for tidal volume.
Algorithms or biomarkers in patients with lower DGBI?
Authors: Camilleri Michael , Yang David Yi 
Published: June 2024

AbstractBackgroundSeveral organizations have proposed guidelines or clinical decision tools for the management of patients with disorders of gut‐brain interactions (DGBI) affecting the lower digestive tract including irritable bowel syndrome and chronic idiopathic constipation. Such algorithms are based on sequential therapeutic trials and modifying the treatment strategy based on efficacy and adverse events.PurposeThe aims of this review are to evaluate the evidence for efficacy of second‐ and third‐line pharmacotherapies and to assess the evidence for the alternative option to manage subgroups of patients with symptoms suggestive of lower DGBI based on diagnostic tests or documented dysfunctions. The preeminent tests to identify such subgroups that present with symptoms that overlap with lower DGBI are detailed: digital rectal examination as well as anorectal manometry and balloon expulsion for evacuation disorders, detailed measurements of colonic transit, and diagnosis of bile acid diarrhea or carbohydrate malabsorption based on biochemical measurements. The review also addresses the cost implications of screening to exclude alternative diagnoses and the costs of therapy associated with the therapeutic options following an algorithmic approach to treatment from the perspective of society, insurer, or patient. Finally, the costs of the diagnostic tests to identify actionable biomarkers and the evidence of efficacy of individualized therapy based on formal diagnosis or documentation of abnormal functions are detailed in the review.