bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamics and Evolutionary Conservation of B Complex Protein Recruitment During Spliceosome Activation
Authors: Fu Xingyang , Hoskins Aaron A. 
Published: August 2024

ABSTRACTSpliceosome assembly and catalytic site formation (called activation) involve dozens of protein and snRNA binding and unbinding events. The B-complex specific proteins Prp38, Snu23, and Spp381 have critical roles in stabilizing the spliceosome during conformational changes essential for activation. While these proteins are conserved, different mechanisms have been proposed for their recruitment to spliceosomes. To visualize recruitment directly, we used Colocalization Single Molecule Spectroscopy (CoSMoS) to study the dynamics of Prp38, Snu23, and Spp381 during splicing in real time. These proteins bind to and release from spliceosomes simultaneously and are likely associated with one another. We designate the complex of Prp38, Snu23, and Spp381 as the B Complex Protein (BCP) subcomplex. Under splicing conditions, the BCP associates with pre-mRNA after tri-snRNP binding. BCP release predominantly occurs after U4 snRNP dissociation and after NineTeen Complex (NTC) association. Under low concentrations of ATP, the BCP pre-associates with the tri-snRNP resulting in their simultaneous binding to pre-mRNA. Together, our results reveal that the BCP recruitment pathway to the spliceosome is conserved betweenS. cerevisiaeand humans. Binding of the BCP to the tri-snRNP when ATP is limiting may result in formation of unproductive complexes that could be used to regulate splicing.KEY POINTSPrp38, Snu23, and Spp381 associate together to form the B Complex Proteins (BCP) ComplexDuring yeast spliceosome assembly, the BCP binds after the tri-snRNP and leaves after NTC arrivalAt low ATP, the BCP pre-associates with the tri-snRNP in complexes that are likely unproductive
Recent climate change strongly impacted the population dynamic of a North American insect pest species
Authors: Boulanger Yan , Desaint Adèle , Martel Véronique , Marchand Maryse , Massoda Tonye Salomon , Saint-Amant Rémi , Régnière Jacques 
Published: August 2024

AbstractClimate change is redefining the dynamics of forest ecosystems globally, particularly through its impact on forest pest populations such as the spruce budworm (SBW,Choristoneura fumiferana[Clem.]), a major defoliator in North American boreal forests. This study investigates the shifts in the population dynamics of spruce budworm across its range in response to recent climate change. We used a process-based, temperature-dependent ecophysiological model combined with the ERA5 reanalysis to assess changes in SBW phenology, reproduction rate, winter survival and population growth rates from 1950 to 2022 across North America. Our findings demonstrate a pronounced northward expansion of suitable climate conditions for SBW, accompanied by earlier phenological events and increased reproduction rates in northern regions. Conversely, the southern parts of its range are experiencing increased winter mortality due to warmer temperatures. This study highlights the significant impact of elevated temperatures, particularly during critical developmental windows such as spring and summer, which are pivotal for spruce budworm survival and reproduction. Additionally, our results reveal that the observed shifts in pest dynamics are more strongly driven by climate change than by changes in landscape composition and structure. We estimated that suitable growth rates have shifted northward by over 68 km on average, but this shift reached more than 200 km in the easternmost portions of its range. Climate-induced shift in suitable conditions for SBW underscores the need for adaptive forest management strategies that consider the rapid ecological changes and the potential for increased forest vulnerability due to climatic and biotic stressors. This study provides vital insights that can inform adaptive management ensuring the sustainability of forest ecosystems in the face of ongoing climate change.
Magnetoencephalography dimensionality reduction informed by dynamic brain states
Authors: Cathignol Annie E , Kusch Lionel , Angiolelli Marianna , Troisi Lopez Emahnuel , Polverino Arianna , Romano Antonella , Sorrentino Giuseppe , Jirsa Viktor , Rabuffo Giovanni , Sorrentino Pierpaolo 
Published: August 2024

AbstractComplex spontaneous brain dynamics mirror the large number of interactions taking place among regions, supporting higher functions. Such complexity is manifested in the inter-regional dependencies among signals derived from different brain areas, as observed utilising neuroimaging techniques, like magnetoencephalography. The dynamics of this data produce numerous subsets of active regions at any moment as they evolve. Notably, converging evidence shows that these states can be understood in terms of transient coordinated events that spread across the brain over multiple spatial and temporal scales. Those can be used as a proxy of the “effectiveness” of the dynamics, as they become stereotyped or disorganised in neurological diseases. However, given the high dimensional nature of the data, representing them has been challenging thus far. Dimensionality reduction techniques are typically deployed to describe complex interdependencies and improve their interpretability. However, many dimensionality reduction techniques lose information about the sequence of configurations that took place. Here, we leverage a newly described algorithm, PHATE (Potential of Heat-diffusion for Affinity-based Transition Embedding), specifically designed to preserve the dynamics of the system in the low-dimensional embedding space. We analysed source-reconstructed resting-state magnetoencephalography from 18 healthy subjects to represent the dynamics of the configuration in low-dimensional space. After reduction with PHATE, unsupervised clustering via K-means is applied to identify distinct clusters. The topography of the states is described, and the dynamics are represented as a transition matrix. All the results have been checked against null models, providing a parsimonious account of the large-scale, fast, aperiodic dynamics during resting-state.
Spatiotemporally Programmed Release of Aptamer Tethered Dual Angiogenic Growth Factors
Authors: Rana Deepti , Rouwkema Jeroen 
Published: August 2024

AbstractIn tissue extracellular matrix (ECM), multiple growth factors (GFs) are sequestered through affinity interactions and released as needed by proteases, establishing spatial morphogen gradients in a time-controlled manner to guide cell behavior. Inspired by these ECM characteristics, we developed an “intelligent” biomaterial platform that spatially controls the combined bioavailability of multiple angiogenic GFs, specifically vascular endothelial growth factor (VEGF) and platelet-derived growth factor (PDGF-BB). Utilizing aptamer affinity interactions and complementary sequences within a GelMA matrix, our platform achieves on-demand, triggered release of individual GFs which can be “programmed” in temporally-controlled, repeatable cycles. The platform features stable incorporation of dual aptamers specific for both GFs, functional aptamer-CS molecular recognition in a 3D microenvironment with long-term stability of at least 15 days at physiological temperature, and spatially localized sequestration of individual GFs. Additionally, the system allows differential amounts of GFs to be released from the same hydrogels at different time-points, mimicking dynamic GF presentation in a 3D matrix similar to the native ECM. This flexible control over individual GF release kinetics opens new possibilities for dynamic GF presentation, with adjustable release profiles to meet the spatiotemporal needs of growing engineered tissue.Graphical Abstract
Turning a Kv channel into hot and cold receptor by perturbing its electromechanical coupling
Authors: Pinto-Anwandter Bernardo I , Bassetto Carlos A Z , Latorre Ramon , Bezanilla Francisco 
Published: August 2024

AbstractVoltage-dependent potassium channels (Kv) are extremely sensitive to membrane voltage and play a crucial role in membrane repolarization during action potentials. Kv channels undergo voltage-dependent transitions between closed states before opening. Despite all we have learned using electrophysiological methods and structural studies, we still lack a detailed picture of the energetics of the activation process. We show here that even a single mutation can drastically modify the temperature response of theShakerKv channel. Using rapid cell membrane temperature steps (Tsteps), we explored the effects of temperature on the ILT mutant (V369I, I372L, and S376T) and the I384N mutant. The ILT mutant produces a significant separation between the transitions of the voltage sensor domain (VSD) activation and the I384N uncouples its movement from the opening of the domain (PD). ILT and I384N respond to temperature in drastically different ways. In ILT, temperature facilitates the opening of the channel akin to a “hot” receptor, reflecting the temperature dependence of the voltage sensor’s last transition and facilitating VSD to PD coupling (electromechanical coupling). In I384N, temperature stabilizes the channel closed configuration analogous to a “cold” receptor. Since I384N drastically uncouples the VSD from the pore opening, we reveal the intrinsic temperature dependence of the PD itself. Here, we propose that the electromechanical coupling has either a “loose” or “tight” conformation. In the loose conformation, the movement of the VSD is necessary but not sufficient to efficiently propagate the electromechanical energy to the S6 gate. In the tight conformation the VSD activation is more effectively translated into the opening of the PD. This conformational switch can be tuned by temperature and modifications of the S4 and S4-S5 linker. Our results show that we can modulate the temperature dependence of Kv channels by affecting its electromechanical coupling.
Adaptive immunity to retroelements promotes barrier integrity
Authors: Wells Alexandria C. , Lima-Junior Djalma Souza , Link Verena M. , Smelkinson Margery , Krishnamurthy Siddharth R. , Chi Liang , Segrist Elisha , Rivera Claudia A. , Teijeiro Ana , Bouladoux Nicolas , Belkaid Yasmine 
Published: August 2024

AbstractMaintenance of tissue integrity is a requirement of host survival. This mandate is of prime importance at barrier sites that are constitutively exposed to the environment. Here, we show that exposure of the skin to non-inflammatory xenobiotics promotes tissue repair; more specifically, mild detergent exposure promotes the reactivation of defined retroelements leading to the induction of retroelement-specific CD8+T cells. These T cell responses are Langerhans cell dependent and establish tissue residency within the skin. Upon injury, retroelement-specific CD8+T cells significantly accelerate wound repair via IL-17A. Collectively, this work demonstrates that tonic environmental exposures and associated adaptive responses to retroelements can be coopted to preemptively set the tissue for maximal resilience to injury.
The harmonized information-technology and organizational performance model (HI-TOP)
Authors: Enstroem Rickard , Singh Kang Parminder , Bhawna Bhawna 
Published: August 2024

Purpose
This study introduces the Harmonized Information-Technology and Organizational Performance Model (HI-TOP), which addresses the need for a holistic framework that integrates technology and human dynamics within organizational settings. This approach aims to enhance organizational productivity and employee well-being by aligning technological advancements with human factors in the context of digital transformation.

Design/methodology/approach
Employing a two-phased methodology, the HI-TOP model is developed through a literature review and text mining of industry reports. This approach identifies and integrates critical themes related to ICT integration challenges and opportunities within organizations.

Findings
This research indicates that successful ICT integration requires balancing technological advancements with human-centric considerations, including addressing technostress and promoting skills development. The HI-TOP model’s four components – Workforce Empowerment and Resource Strategy (WERS), Technology-Enhanced Information Architecture (TEIA), Organizational Information Processing Strategy (OIPS) and Knowledge Sharing Platform (KSP) – demonstrate operational and strategic synergy required to achieve enhanced organizational performance and adaptability.

Originality/value
The HI-TOP model contributes to the body of knowledge by providing a structured framework for understanding the interplay between technology and organizational dynamics, with an emphasis on employee well-being and overall organizational performance. Its originality lies in the integrative approach to model development, combining theory with empirical insights from industry data, thus offering actionable guidance for organizations navigating the complexities of digital transformation.
Arsenic adsorption from aqueous solution using graphene oxide-sodium silicate nanocomposite modified by silver nanoparticles: process modeling by response surface methodology (RSM)
Authors: Hassanzadeh Ali , Ghorbani Kalhor Ebrahim , Farhadi Khalil , Abolhasani Jafar 
Published: August 2024

Purpose
This study aims to investigate the efficacy of Ag@GO/Na2SiO3 nanocomposite in eliminating As from aqueous solutions. Employing response surface methodology, the research systematically examines the adsorption process.

Design/methodology/approach
Various experimental parameters including sample pH, contact time, As concentration and adsorbent dosage are optimized to enhance the As removal process.

Findings
Under optimized conditions, the initial As concentration, contact time, pH and adsorbent dosage are determined to be 32 ppm, 50 mins, 6.5 and 0.4 grams, respectively. While the projected removal of As stands at 97.6% under these conditions, practical application achieves a 93% removal rate. Pareto analysis identifies the order of significance among factors as follows: adsorbent dosage > contact time > pH > As concentration.

Practical implications
This study highlights the potential Ag@GO/Na2SiO3 as a promising adsorbent for efficiently removing industrial As from aqueous solutions, and it is likely to have a good sufficiency in the filtration of water and wastewater treatment plans to remove some chemical pollution, including paints and heavy metals.

Originality/value
The simplicity of the nanocomposite preparation method without the need for advanced equipment and the cheapness of the raw materials and its potential ability to remove As are the prominent advantages of this research.
Could oxygen gradient ektacytometry help to detect sickle cell trait carriers at risk for kidney disorders or exercise‐related complications?
Authors: Connes Philippe , Nader Elie 
Published: August 2024

The study of Ellsworth et al. (Br J Haematol, 2024) demonstrated the usefulness of oxygen gradient ektacytometry technique to better identify the physiological parameters that could increase the risk of sickling of red blood cells (RBCs) from sickle cell trait (SCT) carriers. Oxygen gradient ektacytometry combined with pH and osmolality modulations could help in identifying SCT carriers at risk for kidney disorders or exercise‐related complications. Other factors than the percentages of haemoglobin S are probably involved in the propensity of RBCs from SCT carriers to sickle during deoxygenation.Commentary on: Ellsworth et al. Hypertonicity and/or acidosis induce marked rheological changes under hypoxic conditions in sickle trait red blood cells. Br J Haematol 2024 (Online ahead of print). doi: 10.1111/bjh.19669.
Cenozoic Ampelopsis and Nekemias leaves (Vitaceae, Ampelopsideae) from Eurasia: Paleobiogeographic and paleoclimatic implications
Authors: Tosal Aixa , Vicente Alba , Denk Thomas 
Published: August 2024

AbstractWe describe a new species of Ampelopsideae (Vitaceae), Nekemias mucronata sp. nov., from the Rupelian of Cervera (Spain) and revise another fossil species, Ampelopsis hibschii, originally described from Germany. Comparison with extant Ampelopsideae suggests that the North American species Nekemias arborea is most similar to Nekemias mucronata while the East Mediterranean Ampelopsis orientalis is the closest living relative of A. hibschii. Our review of fossil data indicates that, during the Eocene, four species of Ampelopsideae occurred in Eurasia, that is, N. mucronata in the Czech Republic, A. hibschii in Kazakhstan, and two fossil species in the Far East (Ampelopsis cercidifolia and Ampelopsis protoheterophylla). In the Oligocene, a new species, Ampelopsis schischkinii, appeared in Kazakhstan; meanwhile, N. mucronata spread eastwards and southwards, and A. hibschii mainly grew in Central Europe. In the late Oligocene, N. mucronata became a relict in the Iberian Peninsula and Nekemias might have persisted in Western Eurasia until the latest Miocene (“Ampelopsis” abkhasica). The last occurrence of A. hibschii was in the Middle Miocene in Bulgaria, probably a refuge of humid temperate taxa, along with Ampelopsis aff. cordata. Carpological remains suggest that this lineage persisted in Europe at least until the Pleistocene. Our data confirm previous notions of the North Atlantic and Bering land bridges being important dispersal routes for Ampelopsideae. However, such dispersion probably occurred during the Paleogene rather than the Neogene, as previously suggested. A single species of Ampelopsideae, A. orientalis, has survived in Western Eurasia, which appears to have been linked to a biome shift.
Street‐level bureaucrats' perceptions of “the job”: Deviation from professional particularities and micro creation of public value
Authors: Lavee Einat , Cohen Galia 
Published: August 2024

AbstractThe implementation work of street‐level bureaucrats (SLBs) is becoming more complex due to concurrent imperatives. While previous research has focused on SLBs' discretion decisions and coping strategies, this study adds to existing literature by suggesting that their perceptions of “the job” are changing. Drawing on in‐depth interviews with 87 US police officers, we found that SLBs are influenced by the great emphasis on public value, understanding their job in terms of assuming general responsibility for citizens, as public representatives. They adopt various strategies to respond to citizens' needs, providing a vast range of services that deviate from their traditional job. We conclude that SLBs work constantly, formally and informally, to effectively address what is good for the public, in ways that go beyond their professional particularities. We argue that such a broader perspective requires public administration scholars to consider more critically the expanded job perceptions, manifested in expanded professional responsibility.
Patient‐reported outcome measures and health economics in regenerative periodontal therapy: A systematic review and meta‐analysis
Authors: Chen Zhaozhao , Lin Cho‐Ying , Wang Hom‐Lay 
Published: August 2024

AbstractUnderstanding patient responses to periodontal regeneration is crucial. This systematic review and meta‐analysis addressed two key questions: (a) the impact of periodontal regeneration on patient‐reported outcome measures (PROMs) for intrabony and furcation involvement and (b) the cost‐effectiveness of periodontal regeneration for treating periodontal defects. Twenty‐four studies were included, with 20 randomized clinical trials (RCTs) reporting patient‐reported outcomes and five (three RCTs and two economic model‐based studies) reporting cost‐effectiveness outcomes. Results favored regeneration therapy over conventional flap surgery for intrabony defects, showing improvements in qualitative (i.e., amount of regenerated attachment apparatus) and quantitative parameters (i.e., probing and radiographic parameters). In terms of PROMs, regenerative treatments involving barrier membranes resulted in longer chair times and higher rates of complications (such as membrane exposure or edema) compared to flap with biologic agents or access flap alone. Despite this, oral health‐related quality of life improved after both regenerative and extraction procedures. Economically, regeneration remained favorable compared to extraction and replacement or open flap debridement alone for periodontal defects. Single‐flap variants in open flap debridement yielded similar outcomes to regenerative treatment, offering a potentially cost‐effective option. Nevertheless, further discussion on the benefits of less‐invasive flap designs is needed due to the lack of histological evaluation.
Investigation of the Responses of Drilling Parameters at Various Bit Rock Interactions and the Inter-Relation to Drilling Performance
Authors: Abugharara Abdelsalam N. A. , Alwaar Abourawi , Futheiz Ibrahim , Butt Stephen D. 
Published: August 2024

Abstract
Accurately understanding the recorded signals of the outcome drilling parameters, precisely linking them to the applied parameters under the determined surface and downhole conditions, correctly interpreting them for better visualization of the downhole drilling conditions to optimize the applied drilling parameters assist in achieving the best drilling performance, minimizing the costs, and significantly reducing the Non-Productive Time (NPT). The aim of this research is to analyze the responses of the drilling parameters’ signals under various status of Bit Rock Interactions (BRI) through applying an innovative laboratory technique and methodology. The aim also includes making correlations of the input and the output parameters for better understanding the parameters’ interrelationships under various status of the bit rock interactions generated by a continuance drilling through precored multi-diameter holes. The research methodology involved a comprehensive laboratory drilling tests using a Large-scale laboratory Drilling Simulator (LDS) at the drilling Technology Laboratory (DTL) of Memorial University of Newfoundland (MUN). Two rock types were used for the drilling experiments, which include granite and sandstone with high and medium strength, respectively. The whole process involved two stages. Stage one involved a pre-coring process using different diameters of impregnated coring bits. The purpose of this stage was preparing a multi-diameter hole to be tested through drilling using a 5-cutter Polycrystalline Diamond Compact (PDC) bit during stage two, where the continuance drilling occurs under a constant applied parameter of Weight On Bit (WOB) and a Revolution Per Minute (RPM). Core bits were perfectly aligned by utilizing downhole bushings with bearings for centralization and friction elimination. The applied parameters that were kept constant included WOB, RPM, formation type, and water Flow Rate (FR). The process of data analysis was conducted on the outcome drilling parameters that included torque and signal amplitude of WOB with respect to a corresponding bit rock interaction. Results showed very useful information between the responsive torque and the applied WOB signal amplitude at a constant applied WOB and other input drilling parameters. This research found novel results that show the decrease in the responsive torque when the area of contact and the bit-rock interaction increase at a constant WOB. Typically, the torque increases when the area of contact increases as a sign of more bit rock interaction involved. Research methodology, experimental procedure, data analysis and results are reported.