bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biofilm formation on titanium implants counteracted by grafting gallium and silver ions
Authors: Cochis Andrea , Azzimonti Barbara , Della Valle Cinzia , Chiesa Roberto , Arciola Carla Renata , Rimondini Lia 
Published: July 2014

AbstractBiofilm‐associated infections remain the leading cause of implant failure. Thanks to its established biocompatibility and biomechanical properties, titanium has become one of the most widely used materials for bone implants. Engineered surface modifications of titanium able to thwart biofilm formation while endowing a safe anchorage to eukaryotic cells are being progressively developed. Here surfaces of disks of commercial grade 2 titanium for bone implant were grafted with gallium and silver ions by anodic spark deposition. Scanning electron microscopy of the surface morphology and energy dispersive X‐ray spectroscopy were used for characterization. Gallium‐grafted titanium was evaluated in comparison with silver‐grafted titanium for both in vivo and in vitro antibiofilm properties and for in vitro compatibility with human primary gingival fibroblasts. Surface‐modified materials showed: (i) homogeneous porous morphology, with pores of micrometric size; (ii) absence of cytotoxic effects; (iii) ability to support in vitro the adhesion and spreading of gingival fibroblasts; and (iv) antibiofilm properties. Although both silver and gallium exhibited in vitro strong antibacterial properties, in vivo gallium was significantly more effective than silver in reducing number and viability of biofilm bacteria colonies. Gallium‐based treatments represent promising titanium antibiofilm coatings to develop new bone implantable devices for oral, maxillofacial, and orthopedic applications. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1176–1187, 2015.
Microporous calcium phosphate ceramics driving osteogenesis through surface architecture
Authors: Zhang Jingwei , Barbieri Davide , ten Hoopen Hetty , de Bruijn Joost D. , van Blitterswijk Clemens A. , Yuan Huipin 
Published: July 2014

AbstractThe presence of micropores in calcium phosphate (CaP) ceramics has shown its important role in initiating inductive bone formation in ectopic sites. To investigate how microporous CaP ceramics trigger osteoinduction, we optimized two biphasic CaP ceramics (i.e., BCP‐R and BCP‐S) to have the same chemical composition, equivalent surface area per volume, comparable protein adsorption, similar ion (i.e., calcium and phosphate) exchange and the same surface mineralization potential, but different surface architecture. In particular, BCP‐R had a surface roughness (Ra) of 325.4 ± 58.9 nm while for BCP‐S it was 231.6 ± 35.7 nm. Ceramic blocks with crossing or noncrossing channels of 250, 500, 1000, and 2000 µm were implanted in paraspinal muscle of dogs for 12 weeks. The percentage of bone volume in the channels was not affected by the type of pores (i.e., crossing vs. closed) or their size, but it was greatly influenced by the ceramic type (i.e., BCP‐R vs. BCP‐S). Significantly, more bone was formed in the channels of BCP‐R than in those of BCP‐S. Since the two CaP ceramics differed only in their surface architecture, the results hereby demonstrate that microporous CaP ceramics may induce ectopic osteogenesis through surface architecture. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1188–1199, 2015.
Thrombospondin-1 Regulates Bone Homeostasis Through Effects on Bone Matrix Integrity and Nitric Oxide Signaling in Osteoclasts
Authors: Amend Sarah R , Uluckan Ozge , Hurchla Michelle , Leib Daniel , Veis Novack Deborah , Silva Matthew , Frazier William , Weilbaecher Katherine N 
Published: July 2014

Abstract

Thrombospondin-1 (TSP1), an endogenous antiangiogenic, is a widely expressed secreted ligand with roles in migration, adhesion, and proliferation and is a target for new therapeutics. While TSP1 is present in the bone matrix and several TSP1 receptors play roles in bone biology, the role of TSP1 in bone remodeling has not been fully elucidated. Bone turnover is characterized by coordinated activity of bone-forming osteoblasts (OB) and bone-resorbing osteoclasts (OC). TSP1−/− mice had increased bone mass and increased cortical bone size and thickness compared to wild type (WT). However, despite increased size, TSP1−/− femurs showed less resistance to bending than expected, indicative of diminished bone quality and a bone material defect. Additionally, we found that TSP1 deficiency resulted in decreased OC activity in vivo and reduced OC differentiation. TSP1 was critical during early osteoclastogenesis, and TSP1 deficiency resulted in a substantial overexpression of inducible nitric oxide synthase (iNOS). Importantly, administration of a NOS inhibitor rescued the OC function defects of TSP1−/− mice in vivo. To investigate the role of bone-derived TSP1 in osteoclastogenesis, we found that WT pre-OCs had defective iNOS expression when cultured on TSP1−/− bone compared to WT bone, suggesting that TSP1 in bone plays a critical role in iNOS signaling during OC development. These data implicate a new role for TSP1 in bone homeostasis with roles in maintaining bone matrix integrity and regulating OC formation. It will be critical to monitor bone health of patients administered TSP1-pathway directed therapeutics in clinical use and under development. © 2014 American Society for Bone and Mineral Research.
Synthesis, Characterization and Photoluminescence of Lanthanide Metal‐organic Frameworks, Constructed from Triangular 4,4′,4″‐s‐triazine‐1,3,5‐triyl‐p‐aminobenzoate Ligands
Authors: Liao Ju‐Hsiou , Lai Chien‐Yuan , Yang Chih‐Liang 
Published: July 2014

AbstractEight isomorphous metal‐organic frameworks: [Ln2(TATAB)2(H2O)(DMA)6]·5H2O (Ln = Sm (1), Eu (2), Gd (3), Tb (4), Dy (5), Er (6), Tm (7), Yb (8)); TATAB = 4,4′,4″‐s‐triazine‐1,3,5‐triyl‐p‐aminobenzoate, DMA = N,N‐dimethylacetamide), were synthesized by the self‐assembly of lanthanide ions, TATAB, DMA and H2O. Single‐crystal X‐ray crystallography reveals they are three dimensional frameworks with 2‐fold interpenetration. Solid‐state photoluminescence studies indicate ligand‐to‐metal energy transfer is more efficient for compounds 2 and 4 which exhibit intense characteristic lanthanide emissions at room temperature.
An Efficient Method for Chemoselective Reduction of Nitro Compounds Using Bimetallic Fe‐Ni NPs/H3PW12O40.×H2O System
Authors: Rezazadeh Soodabeh , Akhlaghinia Batool , Goharshadi Elaheh K. , Sarvari Hossein 
Published: July 2014

AbstractThe selective reduction of nitro compounds by treatment with bimetallic Fe‐Ni nanoparticles (NPs) and tungestophosphoric acid hydrate (H3PW12O40.×H2O) in H2O is reported. The method has been applied to a broad range of nitro compounds with different sensitive functionalities, including halides, carbonyl, hydroxyl, aldehyde, methyl, acetyl, nitrile, and ester substituents with excellent yields. The reaction yielded single product in all cases with very high yield. The simple experimental procedure and easy purification make the protocol advantageous.
A novel real‐time method to estimate volumetric mass biodensity based on the combination of dielectric spectroscopy and soft‐sensors
Authors: Ehgartner Daniela , Sagmeister Patrick , Herwig Christoph , Wechselberger Patrick 
Published: July 2014

AbstractBACKGROUNDThe PAT initiative encourages designing, analyzing, and controlling manufacturing through timely measurements. Dielectric measurements and first principle soft‐sensors emerged as valuable real‐time bioprocess analytical PAT tools to improve process understanding and control capabilities.RESULTSThe estimation of a novel physiological variable, bio‐density is presented, using a combination of two PAT sensors, dielectric spectroscopy and a soft‐sensor based on first principle elemental balances. Within this contribution, calibrations for the estimation of biomass dry weight using dielectric measurements were found to change in the transition from batch‐ to fed‐batch and within the induction phase for two recombinant E. coli bioprocesses. This was explained by a change in cell size, as indicated by flow cytometric measurements, light microscopy and changes in the beta dispersion dielectric spectrum. Biomass dry weight was accurately determined using a first principle soft‐sensor. The combination of both signals allowed the real‐time estimation of a new physiological variable, interpreted as bio‐density. Applying this concept, batch/fed‐batch and induction phase physiological changes were successfully observed in real time. Furthermore, a correlation with the specific product titer was observed.CONCLUSIONThis contribution enhances the application spectrum of dielectric measurements and soft‐sensors for the detection of physiological changes and recombinant product estimation, aiming at more efficient design, monitoring and control of bioprocesses as demanded by the process analytical technology (PAT) initiative. © 2014 Society of Chemical Industry
Intravenous mesenchymal stem cell therapy after recurrent laryngeal nerve injury: A preliminary study
Authors: Lerner Michael Z. , Matsushita Takashi , Lankford Karen L. , Radtke Christine , Kocsis Jeffery D. , Young Nwanmegha O. 
Published: July 2014

Objectives/HypothesisIntravenous administration of mesenchymal stem cells (MSCs) has been recently shown to enhance functional recovery after stroke and spinal cord injury. The therapeutic properties of MSCs are attributed to their secretion of a variety of potent antiinflammatory and neurotrophic factors. We hypothesize that intravenous administration of MSCs after recurrent laryngeal nerve (RLN) injury in the rat may enhance functional recovery.Study DesignAnimal Research.MethodsTwelve 250‐gram Sprague‐Dawley rats underwent a controlled crush injury to the left RLN. After confirming postoperative vocal fold immobility, each rat was intravenously infused with either green fluorescent protein‐expressing MSCs or control media in a randomized and blinded fashion. Videolaryngoscopy was performed weekly. The laryngoscopy video recordings were reviewed and rated by a fellowship‐trained laryngologist who remained blinded to the intervention using a 0 to 3 scale.ResultsAt 1 week postinjury, the MSC‐infused group showed a trend for higher average functional recovery scores compared to the control group (2.2 vs 1.3), but it did not reach statistical significance (P value of 0.06). By 2 weeks, however, both groups exhibited complete return of function.ConclusionsThese pilot data indicate that with complete nerve transection by crush injury of the RLN in rat, there is complete recovery of vocal fold mobility at 2 weeks. At 1 week postinjury, animals receiving intravenous infusion of MSCs showed a trend for greater functional recovery, suggesting a potential beneficial effect of MSCs; however, this did not reach statistical significance. Therefore, no definite conclusions can be drawn from these data and further study is required.Level of EvidenceN/A. Laryngoscope, 124:2555–2560, 2014
Tamai zone I fingertip replantation: Is external bleeding obligatory for survival of artery anastomosis‐only replanted digits?
Authors: Chen Ko‐Kang , Hsieh Tung‐Ying , Chang Kao‐Ping 
Published: July 2014

BackgroundDistal fingertip replantation is associated with good functional and aesthetic results. Venous anastomosis is the most challenging procedure. For replantation with an artery anastomosis‐only procedure (no venous anastomosis), some protocols have been designed to relieve venous congestion involve anticoagulation and the creation of wounds for persistent bleeding. This report presents the authors' experience of fingertip survival after artery anastomosis‐only replantation with no persistent external bleeding.MethodsTwelve Tamai zone I fingertip total amputation patients who underwent artery anastomosis‐only replantations were recruited from February 2009 to June 2012. Nerve repair was performed if identified. The patients were not subjected to conventional external bleeding methods. Both the blood color on pinprick and fingertip temperature difference between the replanted and uninjured digits were used as indicators of deteriorated venous congestion.ResultsThe replanted digits of 11 patients survived. The only failed replant exhibited an average temperature difference of more than 6°C compared with the uninjured digits and consistently exhibited darker blood during the pinprick test. All other replants exhibited average temperature differences of less than 6°C.ConclusionsIn these Tamai zone I artery anastomosis‐only replantations, fingertips survived without the use of external bleeding method, indicating that external bleeding is probably not obligatory for survival of artery anastomosis‐only replanted digits distal to Tamai zone I. An increasing temperature difference between the replanted and uninjured digits and darker blood on pinprick may be used as indicators of deteriorating congestion signs. © 2014 Wiley Periodicals, Inc. Microsurgery 34:535–539, 2014.
Susceptibility mapping of air, bone, and calcium in the head
Authors: Buch Sagar , Liu Saifeng , Ye Yongquan , Cheng Yu‐Chung Norman , Neelavalli Jaladhar , Haacke E. Mark 
Published: July 2014

PurposeTo demonstrate the mapping of structures with high susceptibility values, such as the sinuses, bones and teeth, using short echo times.MethodsFour in vivo datasets were collected with a gradient‐echo sequence (TE1 = 2.5 ms, TE2 = 5 ms and TE3 = 7.5 ms). Complex division was performed to remove the phase offset term and generate the phase at TE = 2.5 ms. Susceptibility maps were generated from unwrapped phase images, using a geometry‐constrained iterative algorithm, by preserving phase information in the extracerebral tissues. The susceptibility results were improved by updating the missing phase information inside structures with no signal using the predicted phase at each iteration step. Simulated phase images of a three‐dimensional brain model and tooth phantom were used to validate the proposed method.ResultsImproved susceptibility maps were obtained once the phase information in the extracerebral tissue region was incorporated, for both the model and in vivo data. For in vivo data, the average susceptibilities of air (sphenoid sinus), bone and calcium (teeth) were found to be (in ppm): Δχ(sinus‐tissue) = +9.2 ± 1.3, Δχ(bone‐tissue) = −2.1 ± 0.6 and Δχ(teeth‐tissue) = −3.3 ± 1.2, respectively.ConclusionHigh susceptibility structures with little or no signal can be imaged using quantitative susceptibility mapping and can be used to improve background field removal. Magn Reson Med 73:2185–2194, 2015. © 2014 Wiley Periodicals, Inc.
Rapid Determination of Oligopeptides and Amino Acids in Soybean Protein Hydrolysates using High‐Resolution Mass Spectrometry
Authors: Li Xinxiu , Fan Peihong , Zang Meitong , Xing Jie 
Published: July 2014

ABSTRACTIntroductionSoybean protein hydrolysates (SPHs), especially oligopeptides, have shown a variety of functional properties, including immunomodulatory and anti‐oxidant effects. Soybean protein hydrolysate products have been used as functional ingredients in food, sports nutrition or clinical nutrition. However, the mixture is mostly undefined due to its complex nature, containing peptides and minor amino acids as well as small proteins.ObjectivesTo develop a specific and efficient method for the identification and structural characterisation of oligopeptides in SPHs, and to determine free amino acids in SPHs in the same analytical run, for evaluation of the chemical profile of SPH products.MethodsAccurate mass spectrometry (MS) datasets of SPH samples were recorded on a high‐performance liquid chromatography (HPLC) tandem high‐resolution (HR) MS system. Potential oligopeptides were tentatively characterised based on their elemental compositions and ring double bond equivalent (RDBE) values, as well as HRMS/MS data. The analytical method to determine amino acids was evaluated in terms of linearity, precision, apparent recovery and limits of detection and quantitation.ResultsIn total, 186 oligopeptides spanning the mass range of m/z 200–1500 and three major free amino acids could be determined in SPH samples in a single sample injection. Ninety‐nine oligopeptides were tentatively characterised. The sensitive and specific instrumental performances also permitted the determination of 19 amino acids with a limit of quantitation of ≤ 0.1 μg/mL.ConclusionThe HPLC–HRMS technique has proven to be an advantageous tool for the rapid characterisation of oligopeptides and determination of amino acids in soybean protein hydrolysates. Copyright © 2014 John Wiley & Sons, Ltd.
Feasibility and reproducibility of diffusion‐weighted magnetic resonance imaging of the fetal brain in twin–twin transfusion syndrome
Authors: Gomes Neto Orlando , Marins Marcos , Botelho Rafael D. , Nivoloni Rafaela C. , Saura Glauco E. , Vessoni Arias Amábile , Barini Ricardo , Peralta Cleisson F. A. 
Published: July 2014

ABSTRACTObjectiveThe aim of this study is to test the feasibility and reproducibility of diffusion‐weighted magnetic resonance imaging (DW‐MRI) evaluations of the fetal brains in cases of twin–twin transfusion syndrome (TTTS).MethodFrom May 2011 to June 2012, 24 patients with severe TTTS underwent MRI scans for evaluation of the fetal brains. Datasets were analyzed offline on axial DW images and apparent diffusion coefficient (ADC) maps by two radiologists. The subjective evaluation was described as the absence or presence of water diffusion restriction. The objective evaluation was performed by the placement of 20‐mm2 circular regions of interest on the DW image and ADC maps. Subjective interobserver agreement was assessed by the kappa correlation coefficient. Objective intraobserver and interobserver agreements were assessed by proportionate Bland–Altman tests.ResultsSeventy‐four DW‐MRI scans were performed. Sixty of them (81.1%) were considered to be of good quality. Agreement between the radiologists was 100% for the absence or presence of diffusion restriction of water. For both intraobserver and interobserver agreement of ADC measurements, proportionate Bland–Altman tests showed average percentage differences of less than 1.5% and 95% CI of less than 18% for all sites evaluated.ConclusionOur data demonstrate that DW‐MRI evaluation of the fetal brain in TTTS is feasible and reproducible. © 2014 John Wiley & Sons, Ltd.
Mode of action analysis for pesticide‐induced rodent liver tumours involving activation of the constitutive androstane receptor: relevance to human cancer risk
Authors: Lake Brian G , Price Roger J , Osimitz Thomas G 
Published: July 2014

AbstractA number of non‐genotoxic chemicals, including some pesticides, have been shown to increase the incidence of liver tumours in rats and/or mice. Frameworks for analysing the modes of action (MOAs) by which chemicals produce liver tumours in rodents and the relevance of such tumour data for human risk assessment have now been established. One common MOA for rodent liver tumour formation by non‐genotoxic chemicals involves activation of the constitutive androstane receptor (CAR). Key and associative events for a CAR‐activation MOA include receptor activation, liver hypertrophy, induction of cytochrome P450 enzyme activities, increased replicative DNA synthesis, altered hepatic foci and liver tumours. While some effects of rodent CAR activators can be observed in human liver, a major species difference is that, unlike rodents, CAR activators do not increase replicative DNA synthesis in human hepatocytes. The CAR‐activation MOA for rodent liver tumour formation is thus not plausible for humans, and hence such compounds do not pose a hepatocarcinogenic hazard for humans. © 2014 Society of Chemical Industry
An adaptive multichannel protocol for large‐scale machine‐to‐machine networks
Authors: Yen Chi‐Hsien , Hsu Chen‐Yu , Chou Chun‐Ting 
Published: July 2014

AbstractWith the emergence of Internet of Things (IoT), trillions of devices will soon be interconnected and provide new services. The success of IoT relies on the scalability of underlying machine‐to‐machine communication networks. In this paper, we propose a distributed and adaptive multichannel protocol to address the scalability issue. The proposed protocol is composed of (i) real‐time estimation of competing devices, (ii) adaptive channel access probability, and (iii) asynchronous resource reservation. The three features ensure that channel utilization is maximized even when the number of competing devices and their traffic fluctuate dramatically. Our numerical and simulation results show that the proposed protocol can achieve a channel utilization of up to 93%, especially when the number of competing machine is large. Copyright © 2014 John Wiley & Sons, Ltd.
Laparoskopisch-thorakotomische Ösophagusresektion mit intrathorakaler Ösophagogastrostomie als Hybridverfahren
Authors: Hoeppner J. , Marjanovic G. , Glatz T. , Kulemann B. , Hopt U.T. 
Published: July 2014

Zusammenfassung

Hintergrund
In den vergangenen Jahrzehnten wurden verschiedene Varianten der Ösophagusresektion und Rekonstruktion für die kurative Therapie des Ösophaguskarzinoms beschrieben und technisch weiterentwickelt. Insbesondere die minimal-invasiven Verfahren kommen in den letzten Jahren zunehmend zum Einsatz. Die Arbeit stellt die Technik der laparoskopisch-thorakotomischen En-bloc-Ösophagusresektion mit intrathorakaler Ösophagogastrostomie als Hybridverfahren vor (HMIÖ).

Patienten und Methoden
Am Universitätsklinikum Freiburg wurde von Mai 2013 bis April 2014 bei 23 Patienten mit Ösophaguskarzinom eine Ösophagusresektion durchgeführt. Hiervon erfolgte der Eingriff bei 13 Patienten als offene Ösophagusresektion (OÖ) und bei 10 Patienten in HMIÖ-Technik.

Ergebnisse
Die Operationstechnik der HMIÖ wird detailliert in den einzelnen Operationsschritten beschrieben. Es konnte bei der HMIÖ eine R0-Resektionsrate von 100 % erreicht werden sowie eine mit der OÖ vergleichbare Lymphknotenzahl bei der Lymphadenektomie (Median 29 vs. 27). Operationsdauer (Median 347 vs. 412 min), Intensivaufenthalt (Median 6 vs. 9 Tage) und Krankenhausaufenthaltsdauer (Median 13 vs. 17 Tage) stellten sich bei der HMIÖ kürzer dar als bei der OÖ. Die postoperative Gesamtmorbidität (40 % vs. 69 %) und insbesondere die postoperative pulmonale Morbidität (10 % vs. 46 %) waren bei der HMIÖ verringert. Anastomoseninsuffizienzen und postoperative Mortalität traten bei der HMIÖ im eigenen Kollektiv nicht auf.

Schlussfolgerung
Die Technik der HMIÖ vereint Vorteile minimal-invasiver Zugänge auf die postoperative pulmonale Morbidität mit der Sicherheit einer konventionellen Rekonstruktion und Anastomose. Weitere Vorteile ergeben sich durch eine verringerte Operationsdauer und Krankenhausverweildauer.