bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efek Antijamur Ekstrak Daun Selasih Liar (Ocimum gratissimum) pada Fusarium oxysporum f. sp. cepae dan Alternaria porri pada Bawang Merah The antifungal properties of clove basil (Ocimum gratissimum L.) extract on Fusarium oxysporum f. sp. Cepae and Alter
Authors: Nugroho Bambang , Mildaryani Warmanti 
Published: July 2024

The antifungal properties of clove basil (Ocimum gratissimum L.) extract on Fusarium oxysporum f. sp. Cepae and Alternaria porri were evaluated in vitro and in vivo. Thirty grams of the clove basil sterilized leaves were mashed in a sterile mortar, soaked for 24 hr in 100 mL of 96% ethanol as an extractant. The extract was filtered through sterile Whatman No. 1 filter paper and stored at 4°C for subsequent use. Three different concentrations (5, 10, and 15%) were applied using the poisoned food technique. A 4-mm diameter mycelial disc of each tested fungus was inoculated on each amended agar plate. Daily radial growth was recorded for 7 days. For the in vivo test, two different concentrations (15% and 20%) were used. No spray and mancozeb spraying were used as controls. Seed bulbs of shallots were planted in a 25 cm x 25 cm polybag and allowed to be naturally infected by Alternaria porri. The clove basil leaf extract and mancozeb were sprayed onto the plants once a week, starting when the first symptom of the purple blotch disease appeared. The results showed that clove basil leaf extract had good efficacy in inhibiting F. Oxysporum f. sp. Cepae and A. Porri in vitro, with a growth inhibition up to 55%. The extract can also reduce the disease intensity of purple blotch with an infection rate of 0.029, indicating that it has the potential to be developed as a botanical fungicide.

Keywords: antifungal effect, Alternaria porri, clove basil, Fusarium oxysporum f. sp. cepae, leaf extract
L’imagier : une approche pédagogique des controverses à l’université. Aspects politiques, économiques, scientifiques et moyens persuasifs des différents acteurs au sujet de la capture accidentelle des dauphins
Authors: Goldberg Michel , Gustave Marc 
Published: July 2024

Dans trois formations universitaires scientifiques, nous expérimentons l’étude d’une controverse sur les captures accidentelles de dauphins dans le Golfe de Gascogne. Dans notre dispositif (9-12h), les étudiants créent un imagier qui permet de comparer les représentations que proposent les différents acteurs : enjeux, alliances et oppositions, pouvoirs, arguments et moyens de persuasion. Les étudiants découvrent la controverse par des vidéos et des articles qui la contextualisent, tandis que le corpus d’étude qu’ils analysent est principalement constitué de brefs communiqués (500-1000 mots) produits par les acteurs au cours d’un moment discursif particulièrement significatif : une décision du Conseil d’État qui impose des restrictions aux activités de pêche. La réalisation de cet imagier met les étudiants en situation de découvrir les différents acteurs (politiques, industriels, associatifs, scientifiques, médiatiques), ainsi que les questionnements et les points de vue liés au contexte social, culturel et économique de la controverse. Les étudiants analysent également les moyens persuasifs mis en place par ces acteurs pour exercer leur influence.
<em style="mso-bidi-font-style: normal;">Later Plantagenet and the Wars of the Roses Consorts: Power, Influence, and Dynasty. </em>Edited by Aidan Norrie, Carolyn Harris, J.L. Laynesmith, Danna R. Messer, Elena Woodacre. Palgrave Macmillan, 2023. ISBN 978-3-030-94886-3 (ebook). xxiii + 292.&nbsp; $99.00
Authors: Beer Michelle 
Published: July 2024

Reivew of Aidan Norrie, Carolyn Harris, J.L. Laynesmith, Danna R. Messer, and Elena Woodacre, eds. Later Plantagenet and the Wars of the Roses Consorts: Power, Influence and Dynasty (Palgrave Macmillan, 2023).
The Possible Involvement of KATP Channels in Cholestatic Pruritus in Mice
Authors: Afrooghe Arya , Shayan Maryam , Haddadi Nazgol-Sadat , Rashki Asma , Solaimanian Shahabaddin , Zeini Maryam Shokrian , Dehpour Ahmad-Reza 
Published: July 2024

Abstract

Background
Cholestatic itch dramatically impairs the quality of life of affected patients; however, the therapeutic choices are limited and far from sufficient. ATP-sensitive potassium channels (KATP channels) contribute to transmitting signals, including itch. Taking together the modulatory effects of KATP channels on neural excitation and the neurogenic origin of cholestatic itch, we investigated the role of these channels in cholestatic pruritus in mice induced by bile duct ligation.
Results
We detected increased scratching responses in cholestatic mice regarding intradermal vehicle injection, representing cholestatic pruritus. KATP channel openers, diazoxide (10 mg/kg, intraperitoneal), and minoxidil (10 mg/kg, intraperitoneal) attenuated scratching responses in the bile duct ligated mice. On the other hand, KATP channel blocker glibenclamide (3 mg/kg intraperitoneal) intensified the scratching behavior. Moreover, pre-treatment with the sub-effective dose of glibenclamide (1 mg/kg, intraperitoneal) reversed the anti-pruritic effects of diazoxide (10 mg/kg, intraperitoneal) and minoxidil (10 mg/kg intraperitoneal). The open-field test revealed that the scratching behavior was not affected by locomotor activity. Our finding of Quantitative reverse transcription-polymerase chain reaction analysis also showed an increase in the expression of Kcnj11 in bile-duct ligated mice.
Conclusions
We conclude that ATP-sensitive potassium channels are possibly involved in cholestatic itch. Further studies are needed to elucidate the other associations between cholestasis and itch.
Mindfulness predicts reduced aberrant salience and improves stress management
Authors: Wießner Isabel , Souza Júlia Paula , Demarzo Marcelo , Tófoli Luís Fernando 
Published: July 2024

Abstract

Mindfulness can improve mental health and clinical conditions including psychosis, and modulate attentional processes including salience, an automatic mechanism to guide attention towards outstanding elements or ideas. Aberrant salience, the exaggerated assignment of salience, is associated with psychotic experiences, but the effects of mindfulness on aberrant salience are unexplored. To address this, we conducted a pre-post-intervention pilot study, with 21 participants undergoing an 8-week course of Mindfulness-Based Health Promotion (MBHP). Quantitative and qualitative measurements were conducted before (T0), immediately after (T1), and three months after the course (T2). Primary outcome was aberrant salience (Aberrant Salience Inventory, ASI). Secondary outcomes included quantitative scales on mindful attention (Mindful Attention Awareness Scale, MAAS) and decentering (Experiences Questionnaire, EQ), and qualitative reports on participation motivations, preferred practices, learnings, and stress management. Correlations included quantitative changes over time (T1-T0, T2-T0, and T2-T1). The results show that the ASI factor Heightened Cognition increased post-course (T1-T0), while ASI Heightened Emotionality (T2-T0) and total aberrant salience (T2-T1) decreased. MAAS and EQ scores increased (T1-T0, T2-T0). MAAS scores (T1-T0, T2-T0) correlated negatively with ASI Heightened Emotionality (T2-T0). Qualitative analyses indicated consistent stressors but improved stress management, applying the course contents and integrating mindfulness into everyday life, even three months afterward. In conclusion, along with improved well-being and stress management, mindfulness might modify pre-attentional and attentional processes such as reduced aberrant salience, besides enhanced mindful attention and decentering. Importantly, mindful attention might predict and promote aberrant salience reductions, warranting future research on treatment approaches and mechanisms of mindfulness in psychosis.
Comparative Investigation of the Stretch-flange Cracking Mechanism of Ultrahigh-strength Steels with Different Microstructures and Hole-Expansion Ratios
Authors: Matsuki Yuichi , Nakagawa Kinya , Shinmiya Toyohisa , Tamai Yoshikiyo 
Published: July 2024

Abstract

Ultrahigh-strength steel sheets (UHSSs) have been widely adopted for reducing the weight of auto mobile body structures in order to combine fuel efficiency with crashworthiness. One of the issues in the press forming of UHSSs is to prevent stretch-flange cracking on the sheared edges of blank sheets. Although countermeasures have been developed in terms of both materials and processes, the fundamental picture of stretch-flange cracking in diverse types of UHSSs was unclear. In this study, we investigated the mechanism and material factors of stretch-flange cracking in UHSSs with a tensile strength of 980 MPa grade, comparing dual phase(DP), transformation induced plasticity(TRIP) and full martensite(FM) microstructures. The material sheets were pierced by punching or machining, and subsequently, hole-expansion-tested and observed. Macroscopic observation in the tests revealed that the hole-expansion limit was determined by the earliness of crack propagation relative to the hole-expansion ratio. Scanning electron microscope(SEM) analysis of the expanded edge interior showed that void formation occurred exclusively around the crack tip area, thus contributing to ductile crack growth. Microscopy analysis of the expanded edge surfaces revealed the details of stretch-flange cracking. The analysis results suggested that the flange cracks more easily proceeded and cleaved in the order of TRIP, DP, and FM. It was concluded that the crack growth behavior and the hole-expansion limit were dominated by the microstructural resistance to crack growth generated from work-hardened layer.
Approach to systematic improvements of dependability within railway asset management
Authors: Granström Rikard , Söderholm Peter 
Published: July 2024

Abstract

This paper describes results from a research and development (R&D) project at Trafikverket (Swedish transport administration). The purpose of the study was to systemize dependability improvements of Trafikverket’s Control Command and Signalling (CCS) assets. A case study was conducted on level crossings that represent a critical part of the CCS system. The results of the study show that the systemic approach contributes to asset management as it contributes to short-term dependability and productivity improvements as well as to medium term specifications for system modifications and long-term specifications for next generation of level crossings. The approach is based on a combination of methodologies and tools described in dependability standards, e.g., Failure Modes, Effects & Criticality Analysis (FMECA). However, the approach also considers aspects from Design of Experiments (DoE) to support field tests aligned with other tasks in the railway infrastructure. Besides contributing to improvements, the approach complies with regulations and mandatory standards. Examples of these are Common Safety Method for Risk Evaluation and Assessment (CSM-RA, EU 402/2013) and EN 50126 - RAMS (Reliability, Availability, Maintainability & Safety) for railway applications. In addition, the approach complies with regulatory requirements related to enterprise risk management and internal control, i.e., effectiveness, productivity, compliance and documentation. The approach also supports asset management in accordance with the ISO 55000-series.
The Retrotransposon-Derived Capsid Genes PNMA1 and PNMA4 Maintain Reproductive Capacity
Authors: Berchowitz Luke , Wood Thomas , Henriques William , Cullen Harrison , Romero Mayra , Blengini Cecilia , Sarathy Shreya , Sorkin Julia , Bekele Hilina , Jin Chen , Kim Seungsoo , Chemiakine Alexei , Khondker Rishad , Isola Jose , Stout Michael B , Gennarino Vincenzo , Mogessie Binyam , Jain Devanshi , Schindler Karen , Suh Yousin , Wiedenheft Blake 
Published: July 2024

Abstract

The human genome contains 24 gag-like capsid genes derived from deactivated retrotransposons conserved among eutherians. Although some of their encoded proteins retain the ability to form capsids and even transfer cargo, their fitness benefit has remained elusive. Here we show that the gag-like genes PNMA1 and PNMA4 support reproductive capacity during aging. Analysis of donated human ovaries shows that expression of both genes declines normally with age, while several PNMA1 and PNMA4 variants identified in genome-wide association studies are causally associated with low testosterone, altered puberty onset, or obesity. Six-week-old mice lacking either Pnma1 or Pnma4 are indistinguishable from wild-type littermates, but by six months the mutant mice become prematurely subfertile, with precipitous drops in sex hormone levels, gonadal atrophy, and abdominal obesity; overall they produce markedly fewer offspring than controls. These findings expand our understanding of factors that maintain human reproductive health and lend insight into the domestication of retrotransposon-derived genes.
The impact of physiotherapy interventions on respiratory muscle function in patients with neuromuscular disease: A systematic review and meta-analysis
Authors: Ortiz-Garcia Nancy Yesenia , Rueda-Capristran Diego Eduardo , Herrera Domenica Alejandra , Reyes-Rivera Jonathan , Othón-Martínez Diana , Kumar Ajay , Alonso-Ramírez Angie Carolina , Mejia Frances Marie , Martinez-Illan Jonathan David , Cruz Camila Sanchez , Perez-Moreno Elda Janette , Calderón-Martínez Ernesto 
Published: July 2024

Abstract

Background
Neuromuscular diseases (NMDs) represent a diverse group of disorders characterized by progressive impairment of skeletal muscle strength and respiratory function. These disorders pose significant challenges to individuals’ daily activities and overall quality of life. Respiratory complications are common and contribute to high morbidity and mortality rates. Physiotherapy, especially respiratory muscle training, is often recommended as a key intervention to manage and possibly reverse some of these respiratory impairments.
Methods
A systematic review and meta-analysis were conducted to evaluate the effectiveness of physiotherapy interventions on respiratory muscle function in adults with NMDs. The databases searched included Ovid MEDLINE, EMBASE, Web of Science, and others. The outcomes assessed were lung volume and capacity, inspiratory and expiratory muscle strength, and the frequency of respiratory distress episodes.
Results
Of the 7669 identified articles, 25 met the inclusion criteria. Most studies showed a low to moderate risk of bias. The meta-analysis revealed nonsignificant increases in various respiratory parameters, including FVC%, MIP, and MEP. However, a systematic review indicated that more than two-thirds of the study population experienced improved respiratory muscle strength due to physiotherapy interventions. Notably, there was considerable heterogeneity among the studies, which impacted the overall statistical significance of the meta-analysis results.
Conclusion
This review highlights the potential benefits of physiotherapy, particularly RMT, in improving respiratory muscle strength in patients with NMDs. Despite the variability and lack of statistical significance in the meta-analysis, the systematic review suggested that physiotherapy can be a valuable intervention. Future research should aim to standardize interventions and outcome measures to provide more definitive evidence on the efficacy of physiotherapy for respiratory complications in NMD patients.
Optical signature of retinal tau fibrillation
Authors: Salajkova Zita , Barolo Lorenzo , Baiocco Paola , Ruzicka Barbara , Mura Francesco , Lorenzo Francesco Di , Boffi Alberto , Ricco Vincenzo , Ruocco Giancarlo , Leonetti Marco 
Published: July 2024

Abstract

Fibrillated Tau proteins are believed to be a signature of Alzheimer's Disease (AD) and may be potentially employed as a biosensor for AD early detection. Several studies revealed the presence of Tau accumulation and aggregation in the retina, similar to that observed in the brains of individuals with AD. These retinal changes can be non-invasively visualised through AD-related scores derived from reflectance measurements of the patient fundus. However, a direct link with the optical properties of fibrillated protein clusters is still lacking. Here, we present a new optical technique which measures the scattering optical properties of protein fibrils. Our experimental findings show that the scattering intensity of Tau has a wavelength dependence correlated to their size. The optical signal qualitatively replicates the spectral signature observed in human AD patient retinas. Our paper shows that the Tau protein spectral signature is compatible with the distinctive spectral signature of the AD, further confirming that retinal investigation is a promising tool.
The Abl1 tyrosine kinase is a key player in doxorubicin-induced cardiomyopathy and its p53/p73 cell death mediated signaling differs in atrial and ventricular cardiomyocytes
Authors: Borlak Jürgen , Ciribilli Yari , Bisio Alessandra , Selvaraj Saravanakumar , Inga Alberto , Oh Jung-Hwa , Spanel Reinhard 
Published: July 2024

Abstract

Background
Doxorubicin is an important anticancer drug, however, elicits dose-dependently cardiomyopathy. Given its mode of action, i.e. topoisomerase inhibition and DNA damage, we investigated genetic events associated with cardiomyopathy and searched for mechanism-based possibilities to alleviate cardiotoxicity. We treated rats at clinically relevant doses of doxorubicin. Histopathology and transmission electron microscopy (TEM) defined cardiac lesions, and transcriptomics unveiled cardiomyopathy-associated gene regulations. Genomic-footprints revealed critical components of Abl1-p53-signaling, and EMSA-assays evidenced Abl1 DNA-binding activity. Gene reporter assays confirmed Abl1 activity on p53-targets while immunohistochemistry/immunofluorescence microscopy demonstrated Abl1, p53&p73 signaling.
Results
Doxorubicin treatment caused dose-dependently toxic cardiomyopathy, and TEM evidenced damaged mitochondria and myofibrillar disarray. Surviving cardiomyocytes repressed Parkin-1 and Bnip3-mediated mitophagy, stimulated dynamin-1-like dependent mitochondrial fission and induced anti-apoptotic Bag1 signaling. Thus, we observed induced mitochondrial biogenesis. Transcriptomics discovered heterogeneity in cellular responses with minimal overlap between treatments, and the data are highly suggestive for distinct cardiomyocyte (sub)populations which differed in their resilience and reparative capacity. Genome-wide footprints revealed Abl1 and p53 enriched binding sites in doxorubicin-regulated genes, and we confirmed Abl1 DNA-binding activity in EMSA-assays. Extraordinarily, Abl1 signaling differed in the heart with highly significant regulations of Abl1, p53 and p73 in atrial cardiomyocytes. Conversely, in ventricular cardiomyocytes, Abl1 solely-modulated p53-signaling that was BAX transcription-independent. Gene reporter assays established Abl1 cofactor activity for the p53-reporter PG13-luc, and ectopic Abl1 expression stimulated p53-mediated apoptosis.
Conclusions
The tyrosine kinase Abl1 is of critical importance in doxorubicin induced cardiomyopathy, and we propose its inhibition as means to diminish risk of cardiotoxicity.
Study protocol for Optimising patient positioning for accelerated partial breast radiotherapy with the Integrated Magnetic Resonance Linear Accelerator -OPRAH MRL
Authors: Dean Jenna , Anderson Nigel , Halkett Georgia KB , Lye Jessica , Tacey Mark , Foroudi Farshad , Chao Michael , Wright Caroline 
Published: July 2024

Abstract

Background:
Accelerated partial breast irradiation (APBI) is an accepted treatment option for early breast cancer. Treatment delivered on the Magnetic Resonance integrated Linear Accelerator (MRL) provides the added assurance of improved soft tissue visibility, important in the delivery of APBI. This technique can be delivered in both the supine and prone positions, however current literature suggests that prone treatment on the MRL is infeasible due to physical limitations with bore size. This study aims to investigate the feasibility of positioning patients on a custom designed prone breast board compared with supine positioning on a personalised vacuum bag. Geometric distortion, the relative position of Organs at Risk (OAR) to the tumour bed and breathing motion (intrafraction motion) will be compared between the supine and prone positions. The study will also investigate the positional impact on dosimetry, patient experience, and position preference.
Methods:
Up to 30 patients will be recruited over a 12-month period for participation in this Human Research Ethics Committee approved exploratory cohort study. Patients will be scanned on the magnetic resonance imaging (MRI) Simulator in both the supine and prone positions as per current standard of care for APBI simulation. Supine and prone positioning comparisons will all be assessed on de-identified MRI image pairs, acquired using appropriate software. Patient experience will be explored through completion of a short, anonymous electronic survey.
Descriptive statistics will be used for reporting of results with categorical, parametric/non-parametric tests applied (data format dependent). Survey results will be interpreted by comparison of percentage frequencies across the Likert scales. Thematic content analysis will be used to interpret qualitative data from the open-ended survey questions.
Discussion:
The results of this study will be used to assess the feasibility of treating patients with APBI in the prone position on a custom designed board on the MRL. It may also be used to assist with identification of patients who would benefit from this position over supine without the need to perform both scans. Patient experience and technical considerations will be utilised to develop a tool to assist in this process.
Trial Registration:
Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN1262400067583. Registered 28th of May 2024. https://www.anzctr.org.au/ACTRN12624000679583.aspx
The Role of Piezo1 in Regulating Collagen Expression in Orbital Fibroblasts under High Pressure
Authors: Yan Wen , Liu Taixing , Tang Qingqing , Mu Guiying , Li Danwei , Luo Xin 
Published: July 2024

Abstract

Purpose.To explore the effect of Pizeo 1 on the expression of collagen Ⅰ\Ⅲ\Ⅴof orbital fibroblasts in pressure culture simulated the high orbital pressure of thyroid-associated ophthalmopathy (TAO).
Methods.The primary orbital fibroblasts of guinea pig were isolated and cultured by enzymatic digestion method, and the expression levels of Piezo1, α-SMA, and collagenⅠ\Ⅲ\Ⅴin the cells under different pressure (0, 1, 2, and 3Kpa) were checked by WB and PCR. And then the orbital fibroblasts were cultured under a constant pressure of 3KPa and treated with different concentrations (1, 5, and 10 μmol/L) of Piezo1 inhibitor GsMTx4, respectively. The expression levels of Piezo1, α-SMA, and collagenⅠ\Ⅲ\Ⅴ in the orbital fibroblasts were observed. CCK8 and Flow cytometry were used to observe the cell proliferation and apoptosis.
Results.With the increase of pressure, the expression levels of Piezo1 and collagenⅠ\Ⅲ\Ⅴ protein and mRNA in the orbital fibroblasts increased significantly (P< 0.05). Meanwhile, inhibition of Piezo1 by GsMTx4 significantly reduced the levels of OFs transdifferentiation and collagenⅠ\Ⅲ\Ⅴ under the high pressure (P< 0.05). Furthermore, the proliferation of orbital fibroblasts was enhanced with the increase of pressure. Under the co-culture of pressure and inhibitor GsMTx4, the low concentration group promoted the proliferation of orbital fibroblasts, and the high concentration group (10μM) promoted the proliferation of orbital fibroblasts at the early stage, but less than the 5μM group. And at the later stage, the proliferation of orbital fibroblasts was inhibited. Compression alone or compression combined with inhibitor did not affect the apoptosis of orbital fibroblasts.
Conclusion. Down-regulating the expression of piezo1 can inhibit the transdifferentiation of orbital fibroblasts and the synthesis and secretion of collagenⅠ\Ⅲ\Ⅴ, which provide a new idea for exploring the fibrosis mechanism in TAO.