bluuebunny/crossref_metadata_2025_split huggingface dataset:
Luminescent Ruthenium(II) Complex Bearing Bipyridine and N-Heterocyclic Carbene-based C∧N∧C Pincer Ligand for Live-Cell Imaging of Endocytosis
Authors: Tsui Wai-Kuen , Chung Lai-Hon , Wong Matthew Man-Kin , Tsang Wai-Him , Lo Hoi-Shing , Liu Yaxiang , Leung Chung-Hang , Ma Dik-Lung , Chiu Sung-Kay , Wong Chun-Yuen 
Published: March 2015

AbstractLuminescent ruthenium(II)-cyanide complex with N-heterocyclic carbene pincer ligand C∧N∧C = 2,6-bis(1-butylimidazol-2-ylidene)pyridine and 2,2′-bipyridine (bpy) shows minimal cytotoxicity to both human breast carcinoma cell (MCF-7) and human retinal pigmented epithelium cell (RPE) in a wide range of concentration (0.1–500 μM) and can be used for the luminescent imaging of endocytosis of the complex in these cells.
MitoCeption as a new tool to assess the effects of mesenchymal stem/stromal cell mitochondria on cancer cell metabolism and function
Authors: Caicedo Andrés , Fritz Vanessa , Brondello Jean-Marc , Ayala Mickaël , Dennemont Indira , Abdellaoui Naoill , de Fraipont Florence , Moisan Anaïck , Prouteau Claire Angebault , Boukhaddaoui Hassan , Jorgensen Christian , Vignais Marie-Luce 
Published: March 2015

AbstractMitochondrial activity is central to tissue homeostasis. Mitochondria dysfunction constitutes a hallmark of many genetic diseases and plays a key role in tumor progression. The essential role of mitochondria, added to their recently documented capacity to transfer from cell to cell, obviously contributes to their current interest. However, determining the proper role of mitochondria in defined biological contexts was hampered by the lack of suitable experimental tools. We designed a protocol (MitoCeption) to directly and quantitatively transfer mitochondria, isolated from cell type A, to recipient cell type B. We validated and quantified the effective mitochondria transfer by imaging, fluorescence-activated cell sorting (FACS) and mitochondrial DNA analysis. We show that the transfer of minute amounts of mesenchymal stem/stromal cell (MSC) mitochondria to cancer cells, a process otherwise occurring naturally in coculture, results in cancer cell enhanced oxidative phosphorylation (OXPHOS) activity and favors cancer cell proliferation and invasion. The MitoCeption technique, which can be applied to different cell systems, will therefore be a method of choice to analyze the metabolic modifications induced by exogenous mitochondria in host cells.
Correcting positional correlations in Affymetrix® Genome Chips
Authors: Homouz Dirar , Chen Gang , Kudlicki Andrzej S. 
Published: March 2015

AbstractWe report and model a previously undescribed systematic error causing spurious excess correlations that depend on the distance between probes on Affymetrix® microarrays. The phenomenon affects pairs of features with large chip separations, up to over 100 probes apart. The effect may have a significant impact on analysis of correlations in large collections of expression data, where the systematic experimental errors are repeated in many data sets. Examples of such studies include analysis of functions and interactions in groups of genes, as well as global properties of genomes. We find that the average correlations between probes on Affymetrix microarrays are larger for smaller chip distances, which points out to a previously undescribed positional artifact. The magnitude of the artifact depends on the design of the chip and we find it to be especially high for the yeast S98 microarray, where spurious excess correlations reach 0.1 at a distance of 50 probes. We have designed an algorithm to correct this bias and provide new data sets with the corrected expression values. This algorithm was successfully implemented to remove the positional artifact from the S98 chip data while preserving the integrity of the data.
Transport of organic anions and cations in murine embryonic kidney development and in serially-reaggregated engineered kidneys
Authors: Lawrence Melanie L. , Chang C-Hong , Davies Jamie A. 
Published: March 2015

AbstractRecent advances in renal tissue engineering have shown that dissociated, early renogenic tissue from the developing embryo can self-assemble into morphologically accurate kidney-like organs arranged around a central collecting duct tree. In order for such self-assembled kidneys to be useful therapeutically or as models for drug screening, it is necessary to demonstrate that they are functional. One of the main functional characteristics of mature kidneys is transport of organic anions and cations into and out of the proximal tubule. Here, we show that the transport function of embryonic kidneys allowed to develop in culture follows a developmental time-course that is comparable to embryonic kidney development in vivo. We also demonstrate that serially-reaggregated engineered kidneys can transport organic anions and cations through specific uptake and efflux channels. These results support the physiological relevance of kidneys grown in culture, a commonly used model for kidney development and research and suggest that serially-reaggregated kidneys self-assembled from separated cells have some functional characteristics of intact kidneys.
Apoptotic effect of novel Schiff Based CdCl2(C14H21N3O2) complex is mediated via activation of the mitochondrial pathway in colon cancer cells
Authors: Hajrezaie Maryam , Paydar Mohammadjavad , Looi Chung Yeng , Moghadamtousi Soheil Zorofchian , Hassandarvish Pouya , Salga Muhammad Saleh , Karimian Hamed , Shams Keivan , Zahedifard Maryam , Majid Nazia Abdul , Ali Hapipah Mohd , Abdulla Mahmood Ameen 
Published: March 2015

AbstractThe development of metal-based agents has had a tremendous role in the present progress in cancer chemotherapy. One well-known example of metal-based agents is Schiff based metal complexes, which hold great promise for cancer therapy. Based on the potential of Schiff based complexes for the induction of apoptosis, this study aimed to examine the cytotoxic and apoptotic activity of a CdCl2(C14H21N3O2) complex on HT-29 cells. The complex exerted a potent suppressive effect on HT-29 cells with an IC50value of 2.57 ± 0.39 after 72 h of treatment. The collapse of the mitochondrial membrane potential and the elevated release of cytochromecfrom the mitochondria to the cytosol indicate the involvement of the intrinsic pathway in the induction of apoptosis. The role of the mitochondria-dependent apoptotic pathway was further proved by the significant activation of the initiator caspase-9 and the executioner caspases-3 and -7. In addition, the activation of caspase-8, which is associated with the suppression of NF-κB translocation to the nucleus, also revealed the involvement of the extrinsic pathway in the induced apoptosis. The results suggest that the CdCl2(C14H21N3O2) complex is able to induce the apoptosis of colon cancer cells and is a potential candidate for future cancer studies.
Distributed ultrafast fibre laser
Authors: Liu Xueming , Cui Yudong , Han Dongdong , Yao Xiankun , Sun Zhipei 
Published: March 2015

AbstractA traditional ultrafast fibre laser has a constant cavity length that is independent of the pulse wavelength. The investigation of distributed ultrafast (DUF) lasers is conceptually and technically challenging and of great interest because the laser cavity length and fundamental cavity frequency are changeable based on the wavelength. Here, we propose and demonstrate a DUF fibre laser based on a linearly chirped fibre Bragg grating, where the total cavity length is linearly changeable as a function of the pulse wavelength. The spectral sidebands in DUF lasers are enhanced greatly, including the continuous-wave (CW) and pulse components. We observe that all sidebands of the pulse experience the same round-trip time although they have different round-trip distances and refractive indices. The pulse-shaping of the DUF laser is dominated by the dissipative processes in addition to the phase modulations, which makes our ultrafast laser simple and stable. This laser provides a simple, stable, low-cost, ultrafast-pulsed source with controllable and changeable cavity frequency.
Tissue- and time-dependent transcription in Ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host
Authors: Kotsyfakis Michalis , Schwarz Alexandra , Erhart Jan , Ribeiro José M. C. 
Published: March 2015

AbstractIxodes ricinus is a tick that transmits the pathogens of Lyme and several arboviral diseases. Pathogens invade the tick midgut, disseminate through the hemolymph and are transmitted to the vertebrate host via the salivary glands; subverting these processes could be used to interrupt pathogen transfer. Here, we use massive de novo sequencing to characterize the transcriptional dynamics of the salivary and midgut tissues of nymphal and adult I. ricinus at various time points after attachment on the vertebrate host. Members of a number of gene families show stage- and time-specific expression. We hypothesize that gene expression switching may be under epigenetic control and, in support of this, identify 34 candidate proteins that modify histones. I. ricinus-secreted proteins are encoded by genes that have a non-synonymous to synonymous mutation rate even greater than immune-related genes. Midgut transcriptome (mialome) analysis reveals several enzymes associated with protein, carbohydrate and lipid digestion, transporters and channels that might be associated with nutrient uptake and immune-related transcripts including antimicrobial peptides. This publicly available dataset supports the identification of protein and gene targets for biochemical and physiological studies that exploit the transmission lifecycle of this disease vector for preventative and therapeutic purposes.
Optimizing Strontium Ruthenate Thin Films for Near-Infrared Plasmonic Applications
Authors: Braic Laurentiu , Vasilantonakis Nikolaos , Zou Bin , Maier Stefan A. , Alford Neil McN. , Zayats Anatoly V. , Petrov Peter K. 
Published: March 2015

AbstractSeveral new plasmonic materials have recently been introduced in order to achieve better temperature stability than conventional plasmonic metals and control field localization with a choice of plasma frequencies in a wide spectral range. Here, epitaxial SrRuO3 thin films with low surface roughness fabricated by pulsed laser deposition are studied. The influence of the oxygen deposition pressure (20–300 mTorr) on the charge carrier dynamics and optical constants of the thin films in the near-infrared spectral range is elucidated. It is demonstrated that SrRuO3 thin films exhibit plasmonic behavior of the thin films in the near-infrared spectral range with the plasma frequency in 3.16–3.86 eV range and epsilon-near-zero wavelength in 1.11–1.47 μm range that could be controlled by the deposition conditions. The possible applications of these films range from the heat-generating nanostructures in the near-infrared spectral range, to metamaterial-based ideal absorbers and epsilon-near-zero components, where the interplay between real and imaginary parts of the permittivity in a given spectral range is needed for optimizing the spectral performance.
Competing Speech Perception in Middle Age
Authors: Helfer Karen S. 
Published: March 2015

Purpose
This research forum article summarizes research from our laboratory that assessed middle-aged adults' ability to understand speech in the presence of competing talkers.

Method
The performance of middle-aged adults on laboratory-based speech understanding tasks was compared to that of younger and older adults.

Results
Decline in the ability to understand speech in complex listening environments can be demonstrated in midlife. The specific auditory and cognitive contributors to these problems have yet to be established.

Conclusion
There is evidence that the ability to understand a target speech message in the presence of competing speech messages changes relatively early in the aging process. The nature and impact of these changes warrant further investigation.
Listening Comprehension in Middle-Aged Adults
Authors: Sommers Mitchell S. 
Published: March 2015

Purpose
The purpose of this summary is to examine changes in listening comprehension across the adult lifespan and to identify factors associated with individual differences in listening comprehension.

Method
In this article, the author reports on both cross-sectional and longitudinal changes in listening comprehension.

Conclusions
Despite significant declines in both sensory and cognitive abilities, listening comprehension remains relatively unchanged in middle-aged listeners (between the ages of 40 and 60 years) compared with young listeners. These results are discussed with respect to possible compensatory factors that maintain listening comprehension despite impaired hearing and reduced cognitive capacities.
Research Forum on Changes in Sensory Perception in Middle-Aged Adults: A Summary of a Special Session at Hearing Across the Lifespan (HEAL) 2014
Authors: Humes Larry E. 
Published: March 2015

Purpose
The purpose of this article is to introduce the special research forum on sensory-processing changes in middle-aged adults.

Method
This is a brief written introduction to the special session, which included five presentations, each emphasizing a slightly different aspect of sensory perception.

Conclusion
The effects of aging on sensory processing, including auditory processing and speech perception, are not confined to older adults but begin in middle age in many cases.
Age-Related Changes in Cognitive and Sensory Processing: Focus on Middle-Aged Adults
Authors: Humes Larry E. 
Published: March 2015

Purpose
The purpose of this article was to examine the effects of age on (a) various psychophysical measures of threshold sensitivity and temporal processing in hearing, vision, and touch and (b) measures of cognitive processing as assessed by the Wechsler Adult Intelligence Scale–Third Edition (Wechsler, 1997).

Method
Age group differences and correlations with age were examined, as were associations among age, sensory processing, and cognition.

Results
The group analyses showed significant differences on most sensory and cognitive measures such that middle-aged adults performed significantly worse than young adults and significantly better than older adults. Correlations of performance with age were also significant when analyses were restricted to just the young and middle-aged adults. Last, sensory processing, but not age, was significantly correlated with cognitive processing when analyses were restricted to just the young and middle-aged adults.

Conclusion
Middle-aged adults experienced declines in both sensory and cognitive processing. The declines in both the cognitive and sensory domains were such that, for most measures in each domain, the performance of middle-aged adults fell somewhere between that of young and older adults.
Psychometric Evaluation of Lexical Diversity Indices: Assessing Length Effects
Authors: Fergadiotis Gerasimos , Wright Heather Harris , Green Samuel B. 
Published: March 2015

Purpose

Several novel techniques have been developed recently to assess the breadth of a speaker's vocabulary exhibited in a language sample. The specific aim of this study was to increase our understanding of the validity of the scores generated by different lexical diversity (LD) estimation techniques. Four techniques were explored:
D
, Maas, measure of textual lexical diversity, and moving-average type–token ratio.

Method
Four LD indices were estimated for language samples on 4 discourse tasks (procedures, eventcasts, story retell, and recounts) from 442 adults who are neurologically intact. The resulting data were analyzed using structural equation modeling.

Results
The scores for measure of textual lexical diversity and moving-average type–token ratio were stronger indicators of the LD of the language samples. The results for the other 2 techniques were consistent with the presence of method factors representing construct-irrelevant sources.

Conclusion
These findings offer a deeper understanding of the relative validity of the 4 estimation techniques and should assist clinicians and researchers in the selection of LD measures of language samples that minimize construct-irrelevant sources.
The daidzein- and estradiol- induced anorectic action in CCK or leptin receptor deficiency rats
Authors: Fujitani Mina , Mizushige Takafumi , Bhattarai Keshab , Iwahara Asami , Aida Ryojiro , Kishida Taro 
Published: March 2015

AbstractWe investigated the effect of daidzein feeding and estradiol treatment on food intake in cholecystokinin-1 receptor (CCK1R) deficiency, leptin receptor (ObRb) deficiency rats and their wild-type rats. These rats underwent an ovariectomy or a sham operation. For the 5 week experiment, each rat was divided in three groups: control, daidzein (150 mg/kg diet), and estradiol (4.2 μg/rat/day) groups. In both CCK1R+ and CCK1R− rats, daidzein feeding and estradiol treatment significantly decreased food intake. Daidzein feeding significantly reduced food intake in ovariectomized ObRb− rats, although not in ObRb+ rats. Estradiol treatment significantly lowered food intake in ovariectomized ObRb+ and ObRb− rats. In the ovariectomized rats, estradiol treatment significantly increases uterine weight, while daidzein feeding did not change it, suggesting that daidzein might have no or weak estrogenic effect in our experiment. These results suggest that CCK1R and ObRb signalings were not essential for the daidzein- and estradiol-induced anorectic action.
On the boundedness of certain bilinear oscillatory integral operators
Authors: Rodríguez-López Salvador , Rule David , Staubach Wolfgang 
Published: March 2015

We prove the global

L
2

×

L
2

→

L
1

L^2 \times L^2 \to L^1

boundedness of bilinear oscillatory integral operators with amplitudes satisfying a Hörmander-type condition and phases satisfying appropriate growth as well as the strong non-degeneracy conditions. This is an extension of the corresponding result of R. Coifman and Y. Meyer for bilinear pseudodifferential operators, to the case of oscillatory integral operators.
MUGBAS: a species free gene-based programme suite for post-GWAS analysis
Authors: Capomaccio S. , Milanesi M. , Bomba L. , Vajana E. , Ajmone-Marsan P. 
Published: March 2015

Abstract
Genome Wide Association Studies between molecular markers and phenotypes are now routinely run in model and non-model species. However, tools to estimate the probability of association of functional units (e.g. genes) containing multiple markers are not developed for species other than humans. Here we introduce MUGBAS (MUlti species Gene-Based Association Suite), software that estimates the P-value of a gene using information on annotation, single marker GWA results and genotype. The software is species and annotation independent, fast, highly parallelized and ready for high-density marker studies.
Availability and implementation: https://bitbucket.org/capemaster/mugbas
Contact: capemaster@gmail.com
Acinetobacter Infections and Outcomes at an Academic Medical Center: A Disease of Long-Term Care
Authors: Townsend Jennifer , Park An Na , Gander Rita , Orr Kathleen , Arocha Doramarie , Zhang Song , Greenberg David E. 
Published: March 2015

Abstract
Background.  Our study aims to describe the epidemiology, microbial resistance patterns, and clinical outcomes of Acinetobacter infections at an academic university hospital. This retrospective study analyzed all inpatient clinical isolates of Acinetobacter collected at an academic medical center over 4 years. The data were obtained from an Academic tertiary referral center between January 2008 and December 2011. All consecutive inpatients during the study period who had a clinical culture positive for Acinetobacter were included in the study. Patients without medical records available for review or less than 18 years of age were excluded.
Methods.  Records were reviewed to determine source of isolation, risk factors for acquisition, drug resistance patterns, and clinical outcomes. Repetitive sequence-based polymerase chain reaction of selected banked isolates was used to determine patterns of clonal spread in and among institutions during periods of higher infection rates.
Results.  Four hundred eighty-seven clinical isolates of Acinetobacter were found in 212 patients (in 252 admissions). Patients with Acinetobacter infections were frequently admitted from healthcare facilities (HCFs) (59%). One hundred eighty-three of 248 (76%) initial isolates tested were resistant to meropenem. One hundred ninety-eight of 249 (79.5%) initial isolates were multidrug resistant (MDR). Factors associated with mortality included bacteremia (odds ratio [OR] = 1.93, P = .024), concomitant steroid use (OR = 2.87, P &lt; .001), admission from a HCF (OR = 6.34, P = .004), and chronic obstructive pulmonary disease (OR = 3.17, P &lt; .001).
Conclusions.  Acinetobacter isolates at our institution are frequently MDR and are more common among those who reside in HCFs. Our findings underline the need for new strategies to prevent and treat this pathogen, including stewardship efforts in long-term care settings.
Lensing time delays as a substructure constraint: a case study with the cluster SDSS J1004+4112
Authors: Mohammed Irshad , Saha Prasenjit , Liesenborgs Jori 
Published: March 2015

Abstract
Gravitational lensing time delays are well known to depend on cosmological parameters, but they also depend on the details of the mass distribution of the lens. It is usual to model the mass distribution and use time-delay observations to infer cosmological parameters, but it is naturally also possible to take the cosmological parameters as given and use time delays as constraints on the mass distribution. In this paper we develop a method of isolating what exactly those constraints are, using a principal-components analysis of ensembles of free-form mass models. We find that time delays provide tighter constraints on the distribution of matter in the very highly dense regions of the lensing clusters. We apply it to the cluster lens SDSS J1004+4112, whose rich lensing data include two time delays. We find, assuming a concordance cosmology, that the time delays constrain the central region of the cluster to be rounder and less lopsided than would be allowed by lensed images alone. This detailed information about the distribution of the matter is very useful for studying the dense regions of the galaxy clusters which are very difficult to study by direct measurements. A further time-delay measurement, which is expected, will make this system even more interesting.