bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pilot investigations into the mechanistic basis for adverse effects of glucocorticoids in dysferlinopathy
Authors: Lloyd Erin M. , Crew Rachael C. , Haynes Vanessa R. , White Robert B. , Mark Peter J. , Jackaman Connie , Papadimitriou John M. , Pinniger Gavin J. , Murphy Robyn M. , Watt Matthew J. , Grounds Miranda D. 
Published: August 2024

Abstract
Background
Dysferlinopathies are a clinically heterogeneous group of muscular dystrophies caused by gene mutations resulting in deficiency of the membrane-associated protein dysferlin. They manifest post-growth and are characterised by muscle wasting (primarily in the limb and limb-gridle muscles), inflammation, and replacement of myofibres with adipose tissue. The precise pathomechanism for dysferlinopathy is currently unclear; as such there are no treatments currently available. Glucocorticoids (GCs) are widely used to reduce inflammation and treat muscular dystrophies, but when administered to patients with dysferlinopathy, they have unexpected adverse effects, with accelerated loss of muscle strength.

Methods
To investigate the mechanistic basis for the adverse effects of GCs in dysferlinopathy, the potent GC dexamethasone (Dex) was administered for 4–5 weeks (0.5–0.75 µg/mL in drinking water) to dysferlin-deficient BLA/J and normal wild-type (WT) male mice, sampled at 5 (Study 1) or 10 months (Study 2) of age. A wide range of analyses were conducted. Metabolism- and immune-related gene expression was assessed in psoas muscles at both ages and in quadriceps at 10 months of age. For the 10-month-old mice, quadriceps and psoas muscle histology was assessed. Additionally, we investigated the impact of Dex on the predominantly slow and fast-twitch soleus and extensor digitorum longus (EDL) muscles (respectively) in terms of contractile function, myofibre-type composition, and levels of proteins related to contractile function and metabolism, plus glycogen.

Results
At both ages, many complement-related genes were highly expressed in BLA/J muscles, and WT mice were generally more responsive to Dex than BLA/J. The effects of Dex on BLA/J mice included (i) increased expression of inflammasome-related genes in muscles (at 5 months) and (ii) exacerbated histopathology of quadriceps and psoas muscles at 10 months. A novel observation was pronounced staining for glycogen in many myofibres of the damaged quadriceps muscles, with large pale vacuolated myofibres, suggesting possible myofibre death by oncosis.

Conclusion
These pilot studies provide a new focus for further investigation into the adverse effects of GCs on dysferlinopathic muscles.
“I’m not an anxious person”: end-of-life care workers constructing positive psychological states
Authors: Edmonds David Matthew , Zayts-Spence Olga 
Published: August 2024

Abstract
Background
Mental health is an issue of social and economic importance. Sociocultural and scholarly attention has largely focused on the negative aspects of mental health. That is, on mental disorders and illness and how they adversely impact our lives. In contrast, this paper forms part of a recent alternative empirical perspective in discourse-based research, by focusing on the positive aspects of mental health. In this article, we investigate how end-of-life care workers construct their positive psychological states.

Methods
Our data are 38 audio-recorded and transcribed semi-structured interviews with end-of-life care workers from Hong Kong and the United Kingdom. We utilized thematic analysis to identify common categorial strands across the data and discourse analysis to identify the linguistic strategies that these interviewees used to talk about their mental health.

Results
Our thematic analysis generated a superordinate theme across the interviews—namely, that of end-of-life care workers talking about their positive psychological states. We identify three generic ways that end-of-life care workers talked about these psychological states; by “foregrounding the positive,” “reformulating the negative,” and “dismissing the negative.” Our analysis also explicates how interviewees connected social and organizational support to being a benefit to their psychological states.

Conclusions
Our work contributes to existing discourse-based and sociolinguistic research on mental health by turning their focus towards a consideration of its positive dimensions. We also identify recurrent linguistics strategies used by people to construct their mental health. Our analyses point to the importance of investigating mental health as a multidimensional concept that considers participants’ own reflections on their mental health.
Clipped Axillary Node as a Potential Surrogate for Overall Axillary Nodal Status in Inflammatory Breast Cancer Patients after Neoadjuvant Chemotherapy
Authors: Lohani Kush R. , Hoskin Tanya L. , Yasir Saba , Olson Carrie A. , Boughey Judy C. , Hieken Tina J. , Degnim Amy C. 
Published: August 2024

Abstract
Background
Axillary lymph node dissection is the current standard for management of the axilla in inflammatory breast cancer (IBC). The present study aims to determine whether the initially positive node identified by clip placement accurately represents the overall nodal status of axilla after neoadjuvant chemotherapy (NAC) in IBC.

Patients and Methods
A retrospective study was conducted on patients with IBC who underwent operation (2014–2023). For patients with IBC who had clip placement in a positive axillary node at diagnosis, operative notes, specimen radiographs, and pathology reports were reviewed to confirm final pathologic status of clipped nodes.

Results
In total, 92 patients with IBC (90 cN+) were identified (median age 54 years, 78% invasive ductal, 10% invasive lobular, and 12% mixed); 81 (90%) were biopsy-proven cN+, with a clip placed in the positive node for 62/81 (77%). All patients were treated with NAC and axillary surgery with median 19 (range 4–49) nodes removed. Among 28 (out of 56) patients with retrieved clipped nodes that were pathologically negative (ypN0), only 1 had an additional positive node with micrometastasis for a false negative rate of 4% (95% CI 1–19%). Conversely, 3/3 patients with isolated tumor cells (ITCs) only in the clipped node had additional axillary disease (ITCs in 1, macrometastasis in 2), and 20/23 (87%) of patients with pathologically positive clipped node (micrometastasis or greater) had additional positive nodes [19/20 (95%) with macrometastasis].

Conclusions
The clipped biopsy-positive axillary node in IBC accurately represented the post-NAC overall axillary nodal status. ITCs post-NAC should be considered positive as an indicator of additional nodes with metastasis.
Asset-Side Bank Runs and Liquidity Rationing: A Vicious Cycle
Authors: Huang Zongbo 
Published: August 2024

I analyze runs on credit lines in an infinite-horizon banking model, focusing on the strategic complementarity between bankers and credit line borrowers. Panic drawdowns by borrowers coupled with bank liquidity rationing can mutually reinforce each other, creating a vicious cycle. Using data from U.S. banks, I estimate the model and quantify the amplification effect arising from strategic complementarity. This amplification effect accounted for two-thirds of the contraction of bank credit during the 2008–2009 crisis. Moreover, I show that policies targeting borrowers have a crowding-in effect and can effectively contain credit contraction. This paper was accepted by Lukas Schmid, finance. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2023.00872 .
Taking advantage of reference-guided assembly in a slowly-evolving lineage: Application to Testudo graeca
Authors: Mira-Jover Andrea , Graciá Eva , Giménez Andrés , Fritz Uwe , Rodríguez-Caro Roberto Carlos , Bourgeois Yann 
Published: August 2024

Background
Obtaining de novo chromosome-level genome assemblies greatly enhances conservation and evolutionary biology studies. For many research teams, long-read sequencing technologies (that produce highly contiguous assemblies) remain unaffordable or unpractical. For the groups that display high synteny conservation, these limitations can be overcome by a reference-guided assembly using a close relative genome. Among chelonians, tortoises (Testudinidae) are considered one of the most endangered taxa, which calls for more genomic resources. Here we make the most of high synteny conservation in chelonians to produce the first chromosome-level genome assembly of the genus Testudo with one of the most iconic tortoise species in the Mediterranean basin: Testudo graeca.

Results
We used high-quality, paired-end Illumina sequences to build a reference-guided assembly with the chromosome-level reference of Gopherus evgoodei. We reconstructed a 2.29 Gb haploid genome with a scaffold N50 of 107.598 Mb and 5.37% gaps. We sequenced 25,998 protein-coding genes, and identified 41.2% of the assembly as repeats. Demographic history reconstruction based on the genome revealed two events (population decline and recovery) that were consistent with previously suggested phylogeographic patterns for the species. This outlines the value of such reference-guided assemblies for phylogeographic studies.

Conclusions
Our results highlight the value of using close relatives to produce de novo draft assemblies in species where such resources are unavailable. Our annotated genome of T. graeca paves the way to delve deeper into the species’ evolutionary history and provides a valuable resource to enhance direct conservation efforts on their threatened populations.
Lifestyle and mood correlates of cardiometabolic risk in people with serious mental illness on second-generation antipsychotic medications
Authors: Miedlich Susanne U. , Sahay Priya , Olivares Telva E. , Lamberti J. Steven , Morse Diane S. , Brazill Kevin P. , Chhabra Kavaljit H. , Bainbridge Lauren 
Published: August 2024

Introduction
Cardiovascular morbidity and mortality are high in people with serious mental illness (SMI). This problem is mediated, at least in part, by metabolic side effects of second-generation antipsychotics (SGAs) and by unhealthy lifestyle behaviors. We asked whether oral glucose tolerance testing (oGTT) or hemoglobin A1c (HbA1c) is superior in identifying people with SMI at high cardiometabolic risk and whether this risk is shaped by mood, cognition, or lifestyle habits.

Methods
We evaluated 40 patients with schizophrenia, schizoaffective, or bipolar disorder receiving SGAs by oGTT, HbA1c, comprehensive metabolic and lipid panels, and CRP. Mood was assessed using the Patient Health Questionnaire (PHQ-9), and cognition was assessed using the Saint Louis University Mental Status examination. Diet was assessed using the UK Diabetes and Diet Questionnaire (UKDDQ), and physical activity was assessed using daily step counts.

Results
Most patients had prediabetes (preDM) or diabetes mellitus (DM), 72.5% by oGTT, and 52.5% by HbA1c criteria. Pulse rates and insulin resistance indices (Homeostatic Model Assessment of Insulin Resistance, HOMA IR; Matsuda) were significantly different between patients classified as normal or with preDM/DM, using either oGTT or HbA1c criteria. Patients with preDM/DM by HbA1c but not oGTT criteria also had higher waist/hip ratios, triglyceride, and CRP levels (p<0.05). A strong negative correlation was found between average daily step counts and CRP levels (rho = -0.62, p<0.001). Higher UKDDQ scores, or unhealthier diet habits, were associated with higher fasting plasma glucose (rho = 0.28, p = 0.08), triglyceride levels (rho = 0.31, p = 0.05), and insulin resistance (HOMA IR: rho = 0.31, p = 0.06). Higher PHQ-9 scores correlated with lower 2h-oGTT glucose levels (rho = -0.37, p<0.05).

Conclusions
OGTT screening is superior to HbA1c screening in detecting preDM and DM early. Patients identified with preDM/DM by oGTT or HbA1c screening are insulin-resistant and have higher pulse rates. Abdominal obesity, unfavorable lipid profiles, and higher CRP levels were noted in patients screened by HbA1c, but not by oGTT. Low physical activity, low depression scores, and unhealthy diet habits were associated with higher CRP and higher glucose and triglyceride levels, respectively. Future studies should assess the impact of specifically tailored individual lifestyle counseling and medical management interventions in this high-risk population.
The effect of final irrigation with different solutions on smear layer removal and dentin erosion: A scanning electron microscope study
Authors: AlBatati Mohammed , AbuMostafa Ammar 
Published: August 2024

Aim
This study aimed to compare the effectiveness of initial irrigation with sodium hypochlorite (NaOCl) and final irrigation with QMix, 40% citric acid, and 17% ethylenediaminetetraacetic acid (EDTA) on smear layer removal and dentin erosion.

Methodology
Forty extracted human mandibular premolar teeth were randomly divided into four groups (n = 10) according to the type of final irrigants used: 17% EDTA, QMix, citric acid, and control (normal saline). Canals were mechanically prepared using ProTaper Next instruments to an apical size of X3. Subsequently, the roots were sectioned in a buccolingual direction. Scanning electron microscopy (SEM) was used to assess the presence of the smear layer and the amount of dentin erosion in the coronal, middle, and apical thirds of the root canals.

Results
In regards to smear layer removal, there was a significant difference between the control group and the other tested groups. Moreover, it was significantly higher in the coronal and middle thirds than in the apical third. However, there were no significant differences between the groups of EDTA, QMix, and citric acid. Concerning dentin erosion, citric acid produced significantly more dentin erosion than the other tested groups.

Conclusion
Final irrigation with solutions had a higher ability to remove the smear layer in the coronal and middle thirds compared to the apical third. Of all the solutions tested, 40% citric acid had the most pronounced impact on dentin erosion, followed by 17% EDTA and QMix.
Comparing the effects of biguanides and dipeptidyl peptidase-4 inhibitors on cardio-cerebrovascular outcomes, nephropathy, retinopathy, neuropathy, and treatment costs in diabetic patients
Authors: Nakatani Eiji , Ohno Hiromitsu , Satoh Tatsunori , Funaki Daito , Ueki Chikara , Matsunaga Taku , Nagahama Takayoshi , Tonoike Toru , Yui Hiromichi , Miyakoshi Akinori , Tanaka Yoshihiro , Igarashi Ataru , Kumamaru Hiraku , Kuriyama Nagato , Sugawara Akira 
Published: August 2024

Background
Western guidelines often recommend biguanides as the first-line treatment for diabetes. However, dipeptidyl peptidase-4 (DPP-4) inhibitors, alongside biguanides, are increasingly used as the first-line therapy for type 2 diabetes (T2DM) in Japan. However, there have been few studies comparing the effectiveness of biguanides and DPP-4 inhibitors with respect to diabetes-related complications and cardio-cerebrovascular events over the long term, as well as the costs associated.

Objective
We aimed to compare the outcomes of patients with T2DM who initiate treatment with a biguanide versus a DPP-4 inhibitor and the long-term costs associated.

Methods
We performed a cohort study between 2012 and 2021 using a new-user design and the Shizuoka Kokuho database. Patients were included if they were diagnosed with T2DM. The primary outcome was the incidence of cardio-cerebrovascular events or mortality from the initial month of treatment; and the secondary outcomes were the incidences of related complications (nephropathy, renal failure, retinopathy, and peripheral neuropathy) and the daily cost of the drugs used. Individuals who had experienced prior events during the preceding year were excluded, and events within 6 months of the start of the study period were censored. Propensity score matching was performed to compare between two groups.

Results
The matched 1:5 cohort comprised 529 and 2,116 patients who were initially treated with a biguanide or a DPP-4 inhibitor, respectively. Although there were no significant differences in the incidence of cardio-cerebrovascular events or mortality and T2DM-related complications between the two groups (p = 0.139 and p = 0.595), daily biguanide administration was significantly cheaper (mean daily cost for biguanides, 61.1 JPY; for DPP-4 inhibitors, 122.7 JPY; p<0.001).

Conclusion
In patients with T2DM who initiate pharmacotherapy, there were no differences in the long-term incidences of cardio-cerebrovascular events or complications associated with biguanide or DPP-4 use, but the former was less costly.
Os processos dialógicos na concepção do projeto de investigação científica em línguas
Authors: Marra José 
Published: August 2024

A construção de qualquer texto implica a assunção do posicionamento do enunciador que orienta a constituição dos enunciados. Ainda assim, os enunciados realizam-se sob o empreendimento dialógico da língua como seu princípio constitutivo e de ação. O presente artigo objetiva analisar as relações dialógicas entre o conteúdo dos projetos de investigação dos estudantes da língua portuguesa e os respetivos quadros teóricos, considerando a teoria histórico-cultural como sua base epistemológica e as noções de discurso e de texto de Dominique Maingueneau e Mikhael Bakhtin, respetivamente. A metodologia utilizada foi de caracter interpretativo, baseada na qualidade dos nexos de sentido instituídos na relação do investigador com os dados (partes de textos extraídos para a análise). O artigo conclui que os processamentos dialógicos que os estudantes de línguas empreendem na construção do quadro teórico de suas pesquisas resultam maioritariamente de citações diretas às quais ficam subordinadas todas as estratégias argumentativas do texto.
A ciência e suas três histórias
Authors: Moisés Roberto Perides 
Published: August 2024

O processo de fragmentação da comunidade não é um fenômeno recente, produto das relações capitalistas, embora este seja seu primado. Um estudo detalhado e com um determinado viés, nos levará a um momento crucial da formação das comunidades, do humano e da ciência e de seu processo de fragmentação generalizada. A nosso ver, o esgotamento do capital tem oferecido oportunidades de refletirmos sobre projetos civilizatórios que se apresentam na atualidade, tornando evidentes elementos fundamentais que os constituíram. Neste percurso, propomos um debate inicial que pode tornar-se um importante viés na crítica do que temos entendido por Movimento Lógico-histórico. E, neste estado da arte, nem mesmo nossa certeza na compreensão que temos sobre número, numeral e algoritmo escaparão de uma revisão crítica.
Existence of solutions for nonlinear problems involving mixed fractional derivatives with p(x)-Laplacian operator
Authors: Sun Bingzhi 
Published: August 2024

Abstract
In this article, a functional boundary value problem involving mixed fractional derivatives with

p

(

x

)

p\left(x)

-Laplacian operator is investigated. Based on the fixed point theorems and Mawhin’s coincidence theory’s extension theory, some existence theorems are obtained in the case of non-resonance and the case of resonance. Some examples are supplied to verify our main results.
Asymptotic local lower deviations of strictly supercritical branching process in a random environment with geometric distributions of descendants
Authors: Denisov K. Yu. 
Published: August 2024

Abstract
We consider local probabilities of lower deviations for branching process Z

n
= X

n,1 + ⋯ + X

n,Z

n−1
in random environment
η
. We assume that
η
is a sequence of independent identically distributed variables and for fixed
η
the variables X

i,j
are independent and have geometric distributions. We suppose that steps ξ

i
of the associated random walk Sn
= ξ
1 + ⋯ + ξ

n
has positive mean and satisfies left-side Cramér condition: E exp(h
ξi
) < ∞ if h
− < h < 0 for some h
− < − 1. Under these assumptions we find the asymptotic of the local probabilities P(Zn
= ⌊exp(θn)⌋), n → ∞, for θ ∈ (max(m
−, 0); m(− 1)) and for θ in a neighbourhood of m(− 1), where m
− and m(− 1) are some constants.
Right hepatectomy in absence of the left portal vein using the porto-rex shunt procedure
Authors: Lang Sven A. , Bednarsch Jan , Schmitz Sophia M. , Helmedag Marius J. , Amygdalos Iakovos , Heise Daniel , Dewulf Maxime , Ulmer Tom F. , Neumann Ulf P. 
Published: August 2024

Abstract

Objectives
Exact knowledge of the portal vein (PV) anatomy is essential for any hepatobiliary procedure. Absence of the portal bifurcation with the complete blood flow to the left lobe coming from the right portal vein (RPV) is an extremely rare anatomical variation.

Case presentation
In this situation, a solitary metachronous colorectal liver metastasis with suspected infiltration of the RPV and the right bile duct was detected in a 51-year-old male patient. Neither percutaneous ablation nor stereotactic radiotherapy were considered indicated due to the close proximity to the central structures. Hence, a surgical two-step procedure was scheduled. First, a porto-rex shunt with an 8 mm PTFE graft to maintain the portal blood flow to the left lobe was performed. In addition, the RPV was ligated during the same procedure. After recovery, the procedure was completed with a right hepatectomy. The final pathological report confirmed invasion of the right bile duct and the RPV and resection margins were tumor-free.

Conclusion
This case shows that careful preoperative assessment of vascular anatomy is critical. The use of the porto-rex shunt allowed a potentially curative resection in an otherwise irresectable situation.
Acute hyper-hypoxia accelerates the development of depression in mice via the IL-6/PGC1α/MFN2 signaling pathway
Authors: Yu Jialu 
Published: August 2024

Abstract

Background
Neural cell damage is an important cause of exacerbation of depression symptoms caused by hypoxia, but the mechanism behind it is still unclear. The purpose of this study is to elucidate the role of peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α)/mitofusin-2 (MFN2) signaling axis in the development of depression in mice under hypoxia.

Methods
Male Institute of Cancer Research mice (age, 6 weeks) were assigned to the normal group, chronic unpredictable mild stress group (CUMS group), or CUMS + hyper-hypoxia group (CUMS + H group). Mice in the CUMS and CUMS + H groups were exposed to CUMS for 28 days. Additionally, mice in the CUMS + H group were exposed to acute hyper-hypoxia from Day 21 for 7 days. After a total of 28 days, behavioral experiments were conducted. All mice were anesthetized and sacrificed. Levels of brain tissue interleukin (IL)-6, reactive oxygen species (ROS), adenosine triphosphate (ATP), and serotonin (5-HT) were analyzed.

Results
As compared to the CUMS group, mice in the CUMS + H group had increased IL-6 and ROS levels, but lower open-field activity, preference for sucrose, hippocampal neuronal membrane potential, ATP, and 5-HT levels, as well as MFN2 and PGC1α levels.

Conclusions
Acute hyper-hypoxia plays an important role in the development of depression via the IL-6/PGC1α/MFN2 signaling pathway.
The crystal structure of (Z)-3′-(2-(1-(3,4-dimethyl-phenyl)-3-methyl-5-oxo-1,5-dihydro-4H-pyrazol-4-ylidene)hydrazinyl)-2′-hydroxy-[1,1′-biphenyl]-3-carboxylicacid ─ methanol (1/1), C26H26N4O5
Authors: Guo Xiao , Li Tiejun , Cao Xueli , Zhang Wenhao 
Published: August 2024

Abstract
C26H26N4O5, triclinic, P

1
‾

$\overline{1}$

(no. 2), a = 7.245(2) Å, b = 12.522(3) Å, c = 13.789(3) Å, α = 75.420(1)°, β = 79.628(1)°, γ = 83.226(1)°, V = 1,187.5(5) Å3, Z = 2, R

gt
(F) = 0.0467, wR

ref
(F
2) = 0.1399, T = 298 K.