bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mother–infant attachment and the intergenerational transmission of posttraumatic stress disorder
Authors: Bosquet Enlow Michelle , Egeland Byron , Carlson Elizabeth , Blood Emily , Wright Rosalind J. 
Published: September 2013

AbstractEvidence for the intergenerational transmission of posttraumatic stress disorder (PTSD) is documented in the literature, although the underlying mechanisms are poorly understood. Attachment theory provides a framework for elucidating the ways in which maternal PTSD may increase offspring PTSD vulnerability. The current study utilized two independent prospective data sets to test the hypotheses that (a) maternal PTSD increases the probability of developing an insecure mother–infant attachment relationship and (b) an insecure mother–infant attachment relationship increases the risk of developing PTSD following trauma exposure in later life. In the first study of urban, primarily low-income ethnic/racial minority mothers and infants (N= 45 dyads), elevated maternal PTSD symptoms at 6 months were associated with increased risk for an insecure, particularly disorganized, mother–infant attachment relationship at 13 months. In the second birth cohort of urban, low-income mothers and children (N= 96 dyads), insecure (avoidant or resistant) attachment in infancy was associated in a dose–response manner with increased lifetime risk for a diagnosis of PTSD by adolescence. A history of disorganized attachment in infancy predicted severity of PTSD symptoms, including reexperiencing, avoidance, hyperarousal, and total symptoms, at 17.5 years. In both studies, associations between attachment and PTSD were not attributable to numerous co-occurring risk factors. The findings suggest that promoting positive mother–child relationships in early development, particularly in populations at high risk for trauma exposure, may reduce the incidence of PTSD.
Exploring the basis for parents’ negative reactions to being informed that their child is overweight
Authors: Gillison Fiona , Beck Fay , Lewitt Joanna 
Published: September 2013

AbstractObjectiveIncreasing parental awareness of childhood obesity is an important part of tackling the issue. However, parents’ negative reactions to being informed that their children are overweight or obese can hinder their engagement with relevant services. The present study aimed to develop a deeper understanding of why parents react negatively, to help commissioners and service providers design services that are more acceptable to them.DesignOpen, qualitative responses to a survey were collected using a postal questionnaire. Responses were analysed using content analysis.SettingOne local authority in south-west England.SubjectsThe sample frame included all parents receiving letters informing them that their child was overweight (91st–98th centile) or very overweight (98th–100th centile) through the UK National Child Measurement Programme in 2012.ResultsForty-five of 313 eligible parents (14 %) responded to the survey, of whom forty-three rejected either to the judgement that their child was overweight and/or being provided with this feedback. Primary reasons for objection included: lack of trust in the measures used, lack of belief that being overweight is important for children's health (relative to a healthy lifestyle), and fear that discussing weight with children will trigger eating disorders. In addition, parents’ responses suggested that they considered receiving this feedback to be a criticism of their parenting skills.ConclusionsOverall, three areas for improving communication with parents were suggested: tailoring letters; providing information about the importance of weight independently of lifestyle; and addressing parents’ concerns about the risks of talking to children about their weight.
Climate Policy and the United States System of Divided Powers: Dealing with Carbon Leakage and Regulatory Linkage
Authors: Farber Daniel A. 
Published: September 2013

AbstractClimate change has pushed governmental authorities within the United States (US) into new routes of national and transnational policy-making. The normal route for national policy-making runs from Congress in setting policy, to the President in agency implementation, to judicial oversight and enforcement. When that route is blocked, however, federalism and the separation of powers provide some byways and detours that may still be used to make progress. State governments and the executive branch have moved into the breach left by congressional deadlock. In the absence of federal climate legislation or a formal treaty, however, constitutional challenges will predictably meet efforts to limit carbon leakage or to establish linkages between regulatory systems.These constitutional issues often involve corners of constitutional law such as foreign affairs, where doctrines are particularly murky. Solid arguments can be made in favour of state efforts to avoid leakage and create linkage, despite claims of discrimination against interstate commerce, extraterritoriality, and foreign affairs pre-emption. The Environmental Protection Agency has some statutory authority to deal with leakage, and the President seems to have authority to pursue linkage through executive agreement. Thus, both states and the executive branch should have room to deal with transboundary implications of climate policies. Although the deadlock in Congress regarding climate change may be unusually severe, these modes of response may also be important for other kinds of transnational activity by US state governments and the national executive.
Sulfide inhibition of and metabolism by cytochrome c oxidase
Authors: Nicholls Peter , Marshall Doug C. , Cooper Chris E. , Wilson Mike T. 
Published: September 2013

Hydrogen sulfide (H2S), a classic cytochrome c oxidase inhibitor, is also an in vitro oxidase substrate and an in vivo candidate hormonal (‘gasotransmitter’) species affecting sleep and hibernation. H2S, nitric oxide (NO) and carbon monoxide (CO) share some common features. All are low-molecular-mass physiological effectors and also oxidase inhibitors, capable of binding more than one enzyme site, and each is an oxidizable ‘substrate’. The oxidase oxidizes CO to CO2, NO to nitrite and sulfide to probable persulfide species. Mitochondrial cytochrome c oxidase in an aerobic steady state with ascorbate and cytochrome c is rapidly inhibited by sulfide in a biphasic manner. At least two successive inhibited species are involved, probably partially reduced. The oxidized enzyme, in the absence of turnover, occurs in at least two forms: the ‘pulsed’ and ‘resting’ states. The pulsed form reacts aerobically with sulfide to form two intermediates, ‘P’ and ‘F’, otherwise involved in the reaction of oxygen with reduced enzyme. Sulfide can directly reduce the oxygen-reactive a3CuB binuclear centre in the pulsed state. The resting enzyme does not undergo such a step, but only a very slow one-electron reduction of the electron-transferring haem a. In final reactivation phases, both the steady-state inhibition of catalysis and the accumulation of P and F states are reversed by slow sulfide oxidation. A model for this complex reaction pattern is presented.
ProxiMAX randomization: a new technology for non-degenerate saturation mutagenesis of contiguous codons
Authors: Ashraf Mohammed , Frigotto Laura , Smith Matthew E. , Patel Seema , Hughes Marcus D. , Poole Andrew J. , Hebaishi Husam R.M. , Ullman Christopher G. , Hine Anna V. 
Published: September 2013

Back in 2003, we published ‘MAX’ randomization, a process of non-degenerate saturation mutagenesis using exactly 20 codons (one for each amino acid) or else any required subset of those 20 codons. ‘MAX’ randomization saturates codons located in isolated positions within a protein, as might be required in enzyme engineering, or else on one face of an α-helix, as in zinc-finger engineering. Since that time, we have been asked for an equivalent process that can saturate multiple contiguous codons in a non-degenerate manner. We have now developed ‘ProxiMAX’ randomization, which does just that: generating DNA cassettes for saturation mutagenesis without degeneracy or bias. Offering an alternative to trinucleotide phosphoramidite chemistry, ProxiMAX randomization uses nothing more sophisticated than unmodified oligonucleotides and standard molecular biology reagents. Thus it requires no specialized chemistry, reagents or equipment, and simply relies on a process of saturation cycling comprising ligation, amplification and digestion for each cycle. The process can encode both unbiased representation of selected amino acids or else encode them in predefined ratios. Each saturated position can be defined independently of the others. We demonstrate accurate saturation of up to 11 contiguous codons. As such, ProxiMAX randomization is particularly relevant to antibody engineering.
Bacteremia and Mortality with Urinary Catheter–Associated Bacteriuria
Authors: Kizilbash Quratulain F. , Petersen Nancy J. , Chen Guoqing J. , Naik Aanand D. , Trautner Barbara W. 
Published: September 2013

Objective.Although catheter-associated urinary tract infection (CAUTI) and catheter-associated asymptomatic bacteriuria (CAABU) are clinically distinct conditions, most literature describing the risks of bacteriuria does not distinguish between them. We studied the relationship between catheter-associated bacteriuria and bacteremia from a urinary source in CAUTI relative to that in CAABU. Second, we investigated whether the presence or absence of urinary symptoms in catheterized patients with bacteriuria was associated with bacteremia from any source or mortality. Finally, we explored the effect of antimicrobial treatment of bacteriuria on subsequent bacteremia from any source and mortality.Design.We performed a retrospective cohort study with 30 days of follow-up after an initial positive urine culture. CAUTI and CAABU were defined by Infectious Diseases Society of America guidelines.Setting. A large tertiary care facility.Patients.All inpatients with a urinary catheter (external or indwelling) and a positive urine culture between October 2010 and June 2011.Results.We captured 444 episodes of catheter-associated bacteriuria in 308 patients; 128 (41.6%) patients had CAUTI, and 180 (58.4%) had CAABU. Three episodes of bacteriuria were followed by bacteremia from a urinary source (0.7%). CAUTI, rather than CAABU, was associated with bacteremia from any source, but neither CAUTI nor CAABU predicted subsequent mortality. Use of antimicrobial agents to treat bacteriuria was not associated with either bacteremia from any source or mortality.Conclusions.Bacteremia from a urinary source was infrequent, and there was no evidence of an association of mortality with symptomatic versus asymptomatic bacteriuria in this population. Antibiotic treatment of bacteriuria did not affect outcomes.
Gender Equality and Economic Growth: Is there a Win‐Win?
Authors: Kabeer Naila , Natali Luisa 
Published: September 2013

SummaryTo what extent does gender equality contribute to economic growth? And to what extent does the reverse relationship hold true? There are a growing number of studies exploring these relationships, generally using cross‐country regression analysis. They are characterised by varying degrees of methodological rigour to take account of the problems associated with econometric analysis at this highly aggregated level, including the problems of reverse causality. Bearing these problems in mind, a review of this literature suggests that the relationship between gender equality and economic growth is an asymmetrical one. The evidence that gender equality, particularly in education and employment, contributes to economic growth is far more consistent and robust than the relationship that economic growth contributes to gender equality in terms of health, wellbeing and rights. From a growth perspective, therefore, the promotion of certain dimensions of gender equality may appear to offer a win‐win solution but from a gender equity perspective, there is no guarantee that growth on its own will address critical dimensions of gender equality. Either growth strategies would need to be reformulated to be more inclusive in their impacts or redistributive measures would need to be put in place to ensure that men and women benefit more equally from growth.
Pengetahuan Pedagogi Isi Kandungan Guru Sains Sekolah Rendah dalam Mengajarkan Pemikiran Sains Keusahawanan: Satu Kajian Kes
Authors: Syukri Muhammad , Halim Lilia , Mohd. Meerah T. Subahan , Buang Nor Aishah 
Published: September 2013

This qualitative study aims to determine the science teachers’ pedagogical content knowledge (PCK) and its components applied by them in the teaching of entrepreneurial science thinking (EST) by using the EST module. The teaching of entrepreneurial science thinking is an effort to cultivate students’ thinking to apply their scientific knowledge in everyday situation in a creative and inovatif way, including with the orientation of entrepreneurship thinking. Two science teachers, who have educational background and experience in using the EST module to teach science in primary schools, are involved in this study. The qualitative data was analysed by using the six phases of Shulman‘s pedagogical reasoning model (understanding, transformation, instruction, assessment, reflection, and new understanding) in each thinking step in the EST module (observe, new ideas, innovation, creativity, and society). It was found that the PCK component which is always referred to by both teachers is knowledge of students, while the knowledge component of PCK which is seldom applied by the science teachers is knowledge of the contexts. Meanwhile, pedagogical knowledge and content knowledge are applied to all steps of the EST module, but not referred to in all the pedagogical phases of Shulman’s reasoning model. The findings of this study indicate that knowledge of the students is the most important component of teaching EST in primary schools. This study also supports the previous research stated that to enhance creativity and innovation among students, teachers must have knowledge about their students.
Choosing Mathematical Examples: Routine but Not an Easy Task
Authors: Sulaiman Faridah , Mohamed Mohini 
Published: September 2013

Choosing examples for the purpose of teaching mathematics is routine tasks done by every mathematics teachers. Examples are an important medium used by mathematics teachers as a communication device to discuss mathematical content with their students (Leindhart, 2001). Through examples, students build their understanding about mathematical ideas. In spite of the important role played by mathematical examples, the knowledge about mathematical exemplification is not formally taught to the teachers. It has to be built by the teachers through their teaching experience (Zaslavsky & Zodik, 2007). The purpose of this study is to capture this knowledge. We study six Excellent Mathematics Teachers teaching practices using pre-active notes, observation and interview in order to know the things that influence their choice of examples. Findings show that although choosing examples is a routine task, but it seems that it is not an easy one.
Realising Values Through a Probability Activity
Authors: Sivasubramaniam Pumadevi 
Published: September 2013

Values define outlook and attitudes of an individual. Hence, inculcating moral values is of utmost importance to develop the desired behaviour in an individual. Values are a vital part of the affective domain of education including mathematics education. This paper reports a study that aimed to determine the effectiveness of a probability activity in providing experience to students to help them realise the role of the values of ‘humility’, ‘determination’ and ‘perseverance’ in achieving their goals. The comparison of these intended values of the researcher and the students realised values which were based on their written reflections, illustrates the extent to which the activity served its purpose. The results of the study showed that the activity did serve its purpose for the majority of students and hence is suitable to provide experience to students to help them realise the significance of these values.
Infective Endocarditis in Children With Congenital Heart Disease
Authors: Rushani Dinela , Kaufman Jay S. , Ionescu-Ittu Raluca , Mackie Andrew S. , Pilote Louise , Therrien Judith , Marelli Ariane J. 
Published: September 2013

Background—
The American Heart Association guidelines for prevention of infective endocarditis (IE) in 2007 reduced the groups of congenital heart disease (CHD) patients for whom antibiotic prophylaxis was indicated. The evidence base in CHD patients is limited. We sought to determine the risk of IE in children with CHD.

Methods and Results—
We performed a population-based analysis to determine the cumulative incidence and predictors of IE in children (0–18 years) with CHD by the use of the Quebec CHD Database from 1988 to 2010. In 47 518 children with CHD followed for 458 109 patient-years, 185 cases of IE were observed. Cumulative incidence of IE was estimated in the subset of 34 279 children with CHD followed since birth, in whom the risk of IE up to 18 years of age was 6.1/1000 children (95% confidence interval, 5.0–7.5). In a nested case-control analysis, the following CHD lesions were at highest risk of IE in comparison with atrial septal defects (adjusted rate ratio, 95% confidence interval): cyanotic CHD (6.44, 3.95–10.50), endocardial cushion defects (5.47, 2.89–10.36), and left-sided lesions (1.88, 1.01–3.49). Cardiac surgery within 6 months (5.34, 2.49–11.43) and an age of <3 years (3.53, 2.51–4.96; reference, ages 6–18) also conferred an elevated risk of IE.

Conclusions—
In a large population-based cohort of children with CHD, we documented the cumulative incidence of IE and associated factors. These findings help identify groups of patients who are at the highest risk of developing IE.
A systematic review of yoga for state anxiety: Considerations for occupational therapy / Revue systématique sur l’efficacité du yoga pour traiter l’anxiété réactionnelle : Facteurs à considérer en ergothérapie
Authors: Chugh-Gupta Neha , Baldassarre Fulvia G. , Vrkljan Brenda H. 
Published: September 2013

Background. State anxiety can result from a variety of life situations. This type of anxiety can disrupt occupational engagement and performance, thereby affecting rehabilitation and recovery. Occupational therapists need to address the connection between mind-body-spirit and its relationship to performance and engagement in meaningful occupations. Yoga, when used as an adjunct to therapy, has the potential to address state anxiety. Purpose. The aim was to systematically review the evidence concerning the effectiveness of yoga as a treatment approach for state anxiety. Methods. Six electronic databases, the authors’ own files, and the references of included studies from 1990 to July 2011 were searched. Findings. A total of 25 unique studies represented by 26 publications made up the sample: two systematic reviews; 16 randomized controlled trials, and seven prospective, controlled, non-randomized studies. Evidence suggests yoga can be a viable therapeutic option for reducing state anxiety in certain situations. Implications. In making the determination to recommend yoga as an intervention, occupational therapists should consider the client’s circumstances and values as well as the type and intensity of the yoga program.
Paradoxical effect of obesity on hemorrhagic transformation after acute ischemic stroke
Authors: Kim Chi Kyung , Ryu Wi-Sun , Kim Beom Joon , Lee Seung-Hoon 
Published: September 2013

Abstract

Background
Among the patients with established coronary artery diseases, obese patients tend to have a more favorable prognosis, which is called as obesity paradox. Interestingly, mildly obese patients who underwent coronary revascularization had a lower risk of bleeding. In this context, we have investigated the association between obesity and hemorrhagic transformation (HTf) after acute ischemic stroke.

Methods
A total of 365 patients with first-ever acute ischemic stroke were included in this study. Demographic, clinical and radiological information was collected and HTf was evaluated through follow-up T2*-weighted gradient-recalled echo MRI performed usually within 1 week after occurrence of stroke. Body mass index was calculated, and obesity was defined using the World Health Organization Western Pacific Regional Office criteria.

Results
The HTf was identified in 59 patients (16.2%). As the severity of obesity increased, the occurrence of HTf decreased. Compared with the normal weight group and after controlling possible confounders including acute and previous treatment, stroke severity and subtype, the risk of HTf decreased significantly in the obese group (odds ratio, 0.39; 95% confidence interval, 0.17-0.87).

Conclusions
The better outcome for HTf seen in obese patients suggests the existence of a “bleeding-obesity paradox” in acute ischemic stroke.
Samsoeum, a traditional herbal medicine, elicits apoptotic and autophagic cell death by inhibiting Akt/mTOR and activating the JNK pathway in cancer cells
Authors: Kim Aeyung , Yim Nam-Hui , Ma Jin Yeul 
Published: September 2013

Abstract

Background
Samsoeum (SSE), a traditional herbal formula, has been widely used to treat cough, fever, congestion, and emesis for centuries. Recent studies have demonstrated that SSE retains potent pharmacological efficiency in anti-allergic and anti-inflammatory reactions. However, the anti-cancer activity of SSE and its underlying mechanisms have not been studied. Thus, the present study was designed to determine the effect of SSE on cell death and elucidate its detailed mechanism.

Methods
Following SSE treatment, cell growth and cell death were measured using an MTT assay and trypan blue exclusion assay, respectively. Cell cycle arrest and YO-PRO-1 uptake were assayed using flow cytometry, and LC3 redistribution was observed using confocal microscope. The mechanisms of anti-cancer effect of SSE were investigated through western blot analysis.

Results
We initially found that SSE caused dose- and time-dependent cell death in cancer cells but not in normal primary hepatocytes. In addition, during early SSE treatment (6–12 h), cells were arrested in G2/M phase concomitant with up-regulation of p21 and p27 and down-regulation of cyclin D1 and cyclin B1, followed by an increase in apoptotic YO-PRO-1 (+) cells. SSE also induced autophagy via up-regulation of Beclin-1 expression, conversion of microtubule-associated protein light chain 3 (LC3) I to LC3-II, and re-distribution of LC3, indicating autophagosome formation. Moreover, the level of B-cell lymphoma 2 (Bcl-2), which is critical for cross-talk between apoptosis and autophagy, was significantly reduced in SSE-treated cells. Phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) was increased, followed by suppression of the protein kinase B/mammalian target of rapamycin (Akt/mTOR) pathway, and phosphorylation of mitogen-activated protein kinases (MAPKs) in response to SSE treatment. In particular, among MAPKs inhibitors, only the c-Jun N-terminal kinase (JNK)-specific inhibitor SP600125 nearly blocked SSE-induced increases in Beclin-1, LC3-II, and Bax expression and decreases in Bcl-2 expression, indicating that JNK activation plays critical role in cell death caused by SSE.

Conclusions
These findings suggest that SSE efficiently induces cancer cell death via apoptosis as well as autophagy through modification of the Akt/mTOR and JNK signaling pathways. SSE may be as a potent traditional herbal medicine for treating malignancies.