bluuebunny/crossref_metadata_2025_split huggingface dataset:
Folic Acid Functionalized Nanoprobes for Fluorescence‐, Dark‐Field‐, and Dual‐Imaging‐Based Selective Detection of Cancer Cells and Tissue
Authors: Maity Amit Ranjan , Saha Arindam , Roy Arun , Jana Nikhil R. 
Published: January 2013

AbstractFolate‐functionalized quantum dots, gold/silver nanoparticles, and magnetic quantum dots have been synthesized and used as fluorescence, dark‐field, and dual‐imaging probes for the detection of cancer cells and tissues. A very efficient and generalized folate‐functionalization method has been developed for various amine‐terminated nanoparticles. The advantages of the presented approach are that it can be used to synthesize a wide range of folate‐functionalized nanoparticles and the number of folate molecules per nanoparticle can be controlled easily to tune their interaction with folate receptors present at the cell surface. These folate‐functionalized nanoparticles have been used as cellular and tissue‐imaging bioprobes. Results show that folate‐functionalized nanoparticles act as efficient and selective imaging probes in targeting cancer cells and tissues and labeled cells/tissues can be detected by using different imaging modalities.
Executive functioning and behavioral impulsivity of young women who binge eat
Authors: Kelly Nichole R. , Bulik Cynthia M. , Mazzeo Suzanne E. 
Published: January 2013

AbstractObjective:To examine the link between binge eating, executive functioning, and behavioral impulsivity.Method:Fifty women who reported engaging in weekly binge eating in the absence of regular compensatory behaviors and 66 women with no history of binge eating completed several self‐report questionnaires and a brief neuropsychological battery, including the Wisconsin Card Sorting Task and Conner's Continuous Performance Task.Results:Hierarchical regression analyses revealed that groups did not differ in executive functioning after controlling for depression, anxiety, body mass, general intelligence, and psychotropic medication use. Correlation analyses suggest that individuals who endorse more frequent binge eating might have greater difficulties thinking flexibly or shifting attention. Individuals who binge eats are also more likely to behave impulsively, but only for emotional reasons.Discussion:Although this study is unable to determine whether these cognitive and behavioral factors precede or follow binge‐eating episodes, outcomes have implications for treatment and prevention. © 2013 by Wiley Periodicals, Inc. (Int J Eat Disord 2013)
Simulating evolutionary responses of an introgressed insect resistance trait for ecological effect assessment of transgene flow: a model for supporting informed decision‐making in environmental risk assessment
Authors: Meier Matthias S. , Trtikova Miluse , Suter Matthias , Edwards Peter J. , Hilbeck Angelika 
Published: January 2013

AbstractPredicting outcomes of transgene flow from arable crops requires a system perspective that considers ecological and evolutionary processes within a landscape context. In Europe, the arable weed Raphanus raphanistrum is a potential hybridization partner of oilseed rape, and the two species are ecologically linked through the common herbivores Meligethes spp. Observations in Switzerland show that high densities of Meligethes beetles maintained by oilseed rape crops can lead to considerable damage on R. raphanistrum. We asked how increased insect resistance in R. raphanistrum – as might be acquired through introgression from transgenic oilseed rape – would affect seed production under natural herbivore pressure. In simulation experiments, plants protected against Meligethes beetles produced about twice as many seeds as unprotected plants. All stages in the development of reproductive structures from buds to pods were negatively affected by the herbivore, with the transition from buds to flowers being the most vulnerable. We conclude that resistance to Meligethes beetles could confer a considerable selective advantage upon R. raphanistrum in regions where oilseed rape is widely grown.
Co‐Assembly of LDH Nanosheets with Crown Ethers: Structural Transformation and Water‐Adsorption Behavior
Authors: Ma Shulan , Wang Juan , Du Li , Fan Cuihong , Sun Yahong , Sun Genban , Yang Xiaojing 
Published: January 2013

AbstractCo‐assembly of inorganic LDH nanosheets with an organic azacrown ether carboxylic acid derivative was studied by a flocculation method. The homogeneous structure seen in the wet state changed markedly after drying: a staging structure transformed into a homogeneous one after increased assembly times. The experimental observations, changeover from homogeneous to staging and unequal charges of organic and inorganic guests in gallery, might suggest the Daumas–Hérold model applicable in LDH system. The composites with staging could function as powerful water adsorption materials with facile reversibility.
Concise Total Syntheses of Dipiperidine Alkaloids Virgidivarine and Virgiboidine through Ru‐Mediated Ene‐Ene‐Yne Ring Rearrangement Metathesis (RRM)
Authors: Kress Steffen , Weckesser Jochen , Schulz Sabrina Renate , Blechert Siegfried 
Published: January 2013

AbstractHerein, we report on the first enantioselective synthesis of the sparteine‐type alkaloids virgidivarine (1) and virgiboidine (2). The stereoselective construction of the challenging dipiperidine core within 1 and 2 was realized by applying an intramolecular ene‐ene‐yne ring‐rearrangement metathesis (RRM) protocol. In combination with this sequence, an unprecedented tandem metathesis/oxidation reaction was established. Subsequent to the RRM event, the Ru‐alkylidene species was converted into the highly potent dihydroxylation catalyst RuO4 by the addition of NaIO4. This transformation rendered five sequential reactions that include an ene‐ene‐yne RRM/dihydroxylation/glycol cleavage in a one‐pot procedure. This approach provides practical and general access to dipiperidines and piperidino‐quinolizidines. Despite their wide use in organic and organometallic chemistry as well as their occurrence in biologically active natural products, methods are still lacking for the stereoselective synthesis of these motifs.
Evaluation of the saltation process of bed materials by video imaging under altered bed roughness
Authors: Bhattacharyya Anindita , Ojha Satya P. , Mazumder Bijoy S. 
Published: January 2013

ABSTRACTThe purpose of this paper is to examine the nature of particle saltation and movement over the beds of fixed roughness from flume experiments. A series of experiments are carried out to study the saltation of individual sand particles of different sizes over rough beds under different flow conditions. A 3‐D acoustic Doppler velocimeter is used to record the fluid velocity components; subsequently, under different flow conditions, the images of released sand particles are recorded using high‐speed video imaging technique. Systematic analysis is made with regard to the forces acting on the grains and the variation of their magnitudes along the saltation trajectories of the grains. Relations between the saltation parameters, flow intensity and bed roughness are developed. The distributions of the angle of orientations during a single saltation follows almost a Gaussian distribution. The shape of the Gaussian distribution depends on the particle size and bed roughness. Particle collisions with rough beds and the resulting coefficients of restitution are also discussed. A theoretical framework is developed to compute the mean particle velocity considering the spin in the energy balance equation. Results of the detailed analysis using the imaging technique are much better than in previously reported studies. Copyright © 2013 John Wiley & Sons, Ltd.
Increasing rainfall, decreasing winds, and historical changes in Santa Catarina dunefields, southern Brazil
Authors: da Silva Graziela Miot , Hesp Patrick A. 
Published: January 2013

ABSTRACTMorphological and vegetation changes on the Moçambique barrier dunefield system are examined for the period 1938–2002 from aerial photography, and a variety of factors are investigated as possible driving factors. Human factors include a decrease in grazing pressure and tree felling from the early 1960s onwards after 200 years of these activities, and fires. In the 1960s tree planting also took place. During the period 1963 to 1970 there was a marked decline in drift potential (DP – potential sand transport), and then a period of very low DPs (1970–1974). This period falls within the time interval when vegetation cover significantly increased by ~70% along the Moçambique barrier (from 1956 to 1978). During the 1960s to present, the rainfall increased. Analyses of other transgressive dunefields in Santa Catarina and Rio Grande do Sul states show similar trends so it is likely that climatic factors such as increasing rainfall and decreasing DPs are responsible for driving dunefield changes and vegetation colonization of the barriers. Copyright © 2013 John Wiley & Sons, Ltd.
Variation in the Composition and In Vitro Proinflammatory Effect of Urban Particulate Matter from Different Sites
Authors: Manzano‐León Natalia , Quintana Raúl , Sánchez Brisa , Serrano Jesús , Vega Elizabeth , Vázquez‐López Inés , Rojas‐Bracho Leonora , López‐Villegas Tania , O'Neill Marie S. , Vadillo‐Ortega Felipe , De Vizcaya‐Ruiz Andrea , Rosas Irma , Osornio‐Vargas Álvaro R. 
Published: January 2013

ABSTRACTSpatial variation in particulate matter–related health and toxicological outcomes is partly due to its composition. We studied spatial variability in particle composition and induced cellular responses in Mexico City to complement an ongoing epidemiologic study. We measured elements, endotoxins, and polycyclic aromatic hydrocarbons in two particle size fractions collected in five sites. We compared the in vitro proinflammatory response of J774A.1 and THP‐1 cells after exposure to particles, measuring subsequent TNFα and IL‐6 secretion. Particle composition varied by site and size. Particle constituents were subjected to principal component analysis, identifying three components: C1 (Si, Sr, Mg, Ca, Al, Fe, Mn, endotoxin), C2 (polycyclic aromatic hydrocarbons), and C3 (Zn, S, Sb, Ni, Cu, Pb). Induced TNFα levels were higher and more heterogeneous than IL‐6 levels. Cytokines produced by both cell lines only correlated with C1, suggesting that constituents associated with soil induced the inflammatory response and explain observed spatial differences. © 2013 Wiley Periodicals, Inc. J BiochemMol Toxicol 27:87‐97, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21471
Ti(Oi‐Pr)4 Mediated Olefination between Julia Reagent and Aldehydes under Mild Conditions: Facile Synthesis of Vinyl Sulfones
Authors: Yang Jinjin , Yin Wei , Liu Renhua , Chu Changhu 
Published: January 2013

AbstractA novel Ti(Oi‐Pr)4 mediated olifination between Julia reagent and aldehydes has been developed. By this method, a series of substituted vinyl sulfones are prepared under mild conditions, all products are exclusively E isomers. All the structures of these compounds were confirmed by 1H NMR, 13C NMR HRMS and IR.
Imaging features of benign adenomyoepithelioma of the breast
Authors: Park Young Mi , Park Ji Sung , Jung Hyun Seok , Yoon Hye Kyoung , Yang Wei Tse 
Published: January 2013

AbstractPurpose:The purpose of our study was to evaluate the imaging features of benign adenomyoepithelioma of the breast with a focus on sonographic (US) appearances.Methods:Ten benign adenomyoepitheliomas in 9 patients were included in this study. Mammographic and US findings were analyzed retrospectively by 2 radiologists according to the Breast Imaging–Reporting and Data System (BI‐RADS) lexicon. The BI‐RADS final assessment category was also recorded.Results:Of the 8 lesions for which mammography was available, 4 lesions presented with abnormal findings. Two lesions presented with an indistinct, irregular, isodense mass and a circumscribed, oval, isodense mass. The other 2 lesions presented with areas of focal asymmetry and asymmetry, respectively. On US, all of 10 lesions presented as a mass. The masses measured 0.5–1.2 cm (mean, 0.8 cm), were irregular (n = 8) or oval (n = 2) with microlobulated (n = 5), indistinct (n = 3), or angular (n = 2) margins. They were all hypoechoic, had non parallel orientation in 6 cases, and increased peripheral vascularity in 7 cases. The BI‐RADS final assessment category was 4B in 6, and 4A in 4.Conclusions:Mammographic findings of benign adenomyoepithelioma were nonspecific. An irregular, non parallel orientation, microlobulated or indistinct margin, hypoechogenicity, and increased peripheral vascularity were the most frequent US features. © 2013 Wiley Periodicals, Inc. J Clin Ultrasound, 2013
Purified collagen I oriented membrane for tendon repair: An ex vivo morphological study
Authors: Gigante Antonio , Busilacchi Alberto , Lonzi Barbara , Cecconi Stefano , Manzotti Sandra , Renghini Chiara , Giuliani Alessandra , Mattioli‐Belmonte Monica 
Published: January 2013

AbstractInjured tendons have limited repair ability after full‐thickness lesions. Tendon regeneration properties and adverse reactions were assessed ex vivo in an experimental animal model using a new collagen I membrane. The multilamellar membrane obtained from purified equine Achilles tendon is characterized by oriented collagen I fibers and has been shown to sustain cell growth and orientation in vitro. The central third of the patellar tendon (PT) of 10 New Zealand White rabbits was sectioned and grafted with the collagen membrane; the contralateral PT was cut longitudinally (sham‐operated controls). Animals were euthanized 1 or 6 months after surgery, and tendons were subjected to histological and Synchrotron Radiation‐based Computed Microtomography (SRµCT) examination and 3D structure analysis. Histological and SRµCT findings showed satisfactory graft integration with native tendon. Histological examination also showed ongoing angiogenesis. Adverse side‐effects (inflammation, rejection, calcification) were not observed. The multilamellar collagen I membrane can be considered as an effective tool for tendon defect repair and tendon augmentation. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 738–745, 2013
Resurfacing with chemically modified hyaluronic acid and lubricin for flexor tendon reconstruction
Authors: Zhao Chunfeng , Hashimoto Takahiro , Kirk Ramona L. , Thoreson Andrew R. , Jay Gregory D. , Moran Steven L. , An Kai‐Nan , Amadio Peter C. 
Published: January 2013

AbstractWe assessed surface coating with carbodiimide derivatized hyaluronic acid combined with lubricin (cd‐HA‐Lubricin) as a way to improve extrasynovial tendon surface quality and, consequently, the functional results in flexor tendon reconstruction, using a canine in vivo model. The second and fifth flexor digitorum profundus tendons from 14 dogs were reconstructed with autologs peroneus longus (PL) tendons 6 weeks after a failed primary repair. One digit was treated with cd‐HA‐Lubricin, and the other was treated with saline as the control. Six weeks following grafting, the digits and graft tendons were functionally and histologically evaluated. Adhesion score, normalized work of flexion, graft friction in zone II, and adhesion breaking strength at the proximal repair site in zone III were all lower in the cd‐HA‐Lubricin treated group compared to the control group. The strength at the distal tendon/bone interface was decreased in the cd‐HA‐Lubricin treated grafts compared to the control grafts. Histology showed inferior healing in the cd‐HA‐Lubricin group at both proximal and distal repair sites. However, cd‐HA‐Lubricin treatment did not result in any gap or rupture at either the proximal or distal repair sites. These results demonstrate that cd‐HA‐Lubricin can eliminate graft adhesions and improve digit function, but that treatment may have an adverse effect on tendon healing. © 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 31: 969–975, 2013
Shape optimization for the subsidence resistance of an interbody device using simulation‐based genetic algorithms and experimental validation
Authors: Hsu Ching‐Chi 
Published: January 2013

AbstractSubsidence of interbody devices into the vertebral body might result in serious clinical problems, especially when the devices are not well designed and analyzed. Recently, some novel designs were proposed to reduce the risk of subsidence, but those designs are based on the researcher's experience. The purpose of this study was to discover the interbody device design with excellent subsidence resistance by changing the device's shape. The three‐dimensional nonlinear finite element models, which consisted of the interbody device and vertebral body, were created first. Then, the simulation‐based genetic algorithm, which combined the finite element model and the searching algorithm, was developed by using ANSYS® Parametric Design Language. Finally, the numerical results were carefully validated with the use of biomechanical tests. The optimum shape design obtained in this study looks like a flower with many petals and it has excellent subsidence resistance when compared with the other designs provided by the past studies. The results of the present study could help surgeons to understand the subsidence resistance of interbody devices in terms of their shapes and has directly provided the design rationales to engineers. © 2013 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 31:1158–1163, 2013
The use of a novel MUC1 antibody to identify cancer stem cells and circulating MUC1 in mice and patients with pancreatic cancer
Authors: Curry Jennifer M. , Thompson Kyle J. , Rao Shanti G. , Besmer Dahlia M. , Murphy Andrea M. , Grdzelishvili Valery Z. , Ahrens William A. , McKillop Iain H. , Sindram David , Iannitti David A. , Martinie John B. , Mukherjee Pinku 
Published: January 2013

AbstractBackground and ObjectivesMUC1 is over‐expressed and aberrantly glycosylated in >60% of human pancreatic cancer (PC). Development of novel approaches for detection and/or targeting of MUC1 are critically needed and should be able to detect MUC1 on PC cells (including cancer stem cells) and in serum.MethodsThe sensitivity and specificity of the anti‐MUC1 antibody, TAB 004, was determined. CSCs were assessed for MUC1 expression using TAB 004‐FITC on in vitro PC cell lines, and on lineage− cells from in vivo tumors and human samples. Serum was assessed for shed MUC1 via the TAB 004 EIA.ResultsIn vitro and in vivo, TAB 004 detected MUC1 on >95% of CSCs. Approximately, 80% of CSCs in patients displayed MUC1 expression as detected by TAB 004. Shed MUC1 was detected serum in mice with HPAF‐II (MUC1high) but not BxPC3 tumors (MUC1low). The TAB 004 EIA was able to accurately detect stage progression in PC patients.ConclusionsThe TAB 004 antibody may be explored as a therapeutic targeting agent for CSCs in PC. The TAB 004 EIA detected circulating MUC1 in a stage‐dependent manner in patients with PC and thus may be explored as a PC stage diagnostic biomarker. J. Surg. Oncol. 2013;107:713–722. © 2013 Wiley Periodicals, Inc.
Combined pancreas and liver therapies: Resection and ablation in hepato‐pancreatico‐biliary malignancies
Authors: Edwards Jacob , Scoggins Charles , McMasters Kelly , Martin Robert 
Published: January 2013

AbstractBackgroundCombined pancreatic and liver resection for hepato‐pancreatico‐biliary disease is generally considered contraindicated since it is thought to provide little if any survival benefit with high risk of morbidity. Our goal was to review our experience with combined pancreatic and liver resections to better define characteristics that increase risk for perioperative complications after combined resections.MethodsA review of prospectively collected IRB approved hepato‐pancreatico‐biliary database was performed from October 2002 to April 2012.ResultsTwenty‐one cases were identified including all histologies. Perioperative outcomes were examined and the overall mean length of stay was 12.1 days (range: 6–26 days) and no perioperative mortalities (<90 days) were observed. With respect to morbidity 43% of patients experienced any grade of complication, 29% were Grade 3 with no Grade 4 or 5 complications noted. We found a statistically indicated association between BMI > 25 and risk of Grade 3 complications (P = 0.149). The median survival following operation was 11 months (range: 3–148 months).ConclusionCombined pancreas and liver resection for metastatic disease should only be considered in highly selected patients. Tumor histology as well as BMI > 25 (overweight and obese patients) should be considered in the decision making process in an effort to minimize surgical morbidity while potentially improving survival. J. Surg. Oncol. 2013;107:709–712. © 2013 Wiley Periodicals, Inc.
The ToxBank Data Warehouse: Supporting the Replacement of In Vivo Repeated Dose Systemic Toxicity Testing
Authors: Kohonen Pekka , Benfenati Emilio , Bower David , Ceder Rebecca , Crump Michael , Cross Kevin , Grafström Roland C. , Healy Lyn , Helma Christoph , Jeliazkova Nina , Jeliazkov Vedrin , Maggioni Silvia , Miller Scott , Myatt Glenn , Rautenberg Michael , Stacey Glyn , Willighagen Egon , Wiseman Jeff , Hardy Barry 
Published: January 2013

AbstractThe aim of the SEURAT‐1 (Safety Evaluation Ultimately Replacing Animal Testing‐1) research cluster, comprised of seven EU FP7 Health projects co‐financed by Cosmetics Europe, is to generate a proof‐of‐concept to show how the latest technologies, systems toxicology and toxicogenomics can be combined to deliver a test replacement for repeated dose systemic toxicity testing on animals. The SEURAT‐1 strategy is to adopt a mode‐of‐action framework to describe repeated dose toxicity, combining in vitro and in silico methods to derive predictions of in vivo toxicity responses. ToxBank is the cross‐cluster infrastructure project whose activities include the development of a data warehouse to provide a web‐accessible shared repository of research data and protocols, a physical compounds repository, reference or “gold compounds” for use across the cluster (available via wiki.toxbank.net), and a reference resource for biomaterials. Core technologies used in the data warehouse include the ISA‐Tab universal data exchange format, REpresentational State Transfer (REST) web services, the W3C Resource Description Framework (RDF) and the OpenTox standards. We describe the design of the data warehouse based on cluster requirements, the implementation based on open standards, and finally the underlying concepts and initial results of a data analysis utilizing public data related to the gold compounds.