bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prolongation of life by adoptive cell therapy with cascade primed immune cells in four patients with non-small cell lung cancer stages IIIB and IV and a pancoast tumor: a case series
Authors: Laumbacher Barbara , Gu Songhai , Wank Rudolf 
Published: December 2013

Abstract

Introduction
Despite newer treatment modalities, few patients with non-small cell lung cancer in stages IIIB and IV survive the median of one year. We present four patients with non-small cell lung cancer treated with an adjuvant therapy with cascade primed immune cells. The in vitro stimulated expression of cancer information on the patients’ monocytes matures and activates T lymphocytes to destroy cancer cells. The cascade primed immune cell therapy significantly improved the quality of life and the lifespan of all four patients; thus far, three patients survived 40, 55 and 120 months, respectively; and one patient died 39 months after diagnosis.

Case presentation
Patient 1, stage IV (T4N2M1): The adenocarcinoma of the 67-year-old German Caucasian man infiltrated into the mediastinal lymph nodes and iliosacral bones. Chemotherapy modalities were started immediately after diagnosis of cancer, and cascade primed immune cell therapy one year later. The patient survived 39 months.
Patient 2, stage IV (T3N3M1a): The 62-year-old German Caucasian woman presented with adenocarcinoma of the lower lobe with infiltrated lymph nodes of the mediastinum and malignant pleural effusion. Chemotherapy, radiation and the cascade primed immune cell therapy were administered together. The patient is still alive after 40 months.
Patient 3, stage IIIB (T4N1-2M0): The 75-year-old German Caucasian woman presented with an undifferentiated tumor and a separate tumor nodule in the ipsilateral lobe. The patient received only cascade primed immune cell therapy after tumor resection and has survived for the last 55 months.
Patient 4, pancoast tumor (IIIB, T3N3M0): The 77-year-old German Caucasian man presented with an undifferentiated tumor that infiltrated the lymph nodes, the clavicle, one rib and the plexus brachialis. In addition to chemotherapy and radiation, cascade primed immune cells were administered every weekday for one year. After four months, no living tumor cell was detected in the resected lung, the lymph nodes or the bone material. The patient is still alive after 120 months.

Conclusions
The novel adoptive cell therapy with cascade primed immune cells significantly increased the survival rate and maintained the quality of life for four patients with non-small cell lung cancer in stages IIIB and IV. Our findings indicate that tumor resection, chemotherapy and radiation appear to support the cascade primed immune cell therapy.
Effects of grazing intensity on soil labile organic carbon fractions in a desert steppe area in Inner Mongolia
Authors: Cao Jixin , Wang Xiaoping , Sun Xiangyang , Zhang Lin , Tian Yun 
Published: December 2013

Abstract
Grazing can cause changes in soil carbon (C) level. This study aimed to elucidate the response of soil labile organic carbon (SLOC) under four different grazing intensities: non grazing (NG), 0 sheep·ha-1; light grazing (LG), 0.91 sheep·ha-1; moderate grazing (MG), 1.82 sheep·ha-1, and heavy grazing (HG), 2.73 sheep·ha-1. Results showed that there was no significant difference in total soil organic carbon (TOC) and soil inorganic carbon (SIC) content from three soil depths (0-15 cm, 15-30 cm, and 30-45 cm) under different grazing intensities. However, the SLOC including particulate organic carbon (POC), light fraction organic carbon (LFOC), and readily oxidizable carbon (ROC) content at a depth of 0-15 cm decreased with the increasing grazing intensity among LG, MG and HG. The SLOC content at depths of 15-30 cm under the NG and LG were significantly higher than that under the MG and the HG. The TOC and SLOC content decreased with increasing depths of soil horizons, but SIC content increased. The variation trend of the density of different soil carbon fractions and the ratio of individual SLOC fractions to TOC were similar to that of the soil carbon content of corresponding fractions. These results indicated that MG and HG treatments caused C loss at 0-30 cm; and SLOC was more sensitive than TOC in response to different grazing intensities.
Effects of sodium polyacrylate on water retention and infiltration capacity of a sandy soil
Authors: Zhuang Wenhua , Li Longguo , Liu Chao 
Published: December 2013

Abstract
Based on the laboratory study, the effects of sodium polyacrylate (SP) was investigated at 5 rates of 0, 0.08, 0.2, 0.5, and 1%, on water retention, saturated hydraulic conductivity(Ks), infiltration characteristic and water distribution profiles of a sandy soil. The results showed that water retention and available water capacity effectively increased with increasing SP rate. The Ks and the rate of wetting front advance and infiltration under certain pond infiltration was significantly reduced by increasing SP rate, which effectively reduced water in a sandy soil leaking to a deeper layer under the plough layer. The effect of SP on water distribution was obviously to the up layer and very little to the following deeper layers. Considering both the effects on water retention and infiltration capacity, it is suggested that SP be used to the sandy soil at concentrations ranging from 0.2 to 0.5%.
Temporal variations of ecological security with soil and water loss stress in black soil region of northeast China: a case study on Baiquan County
Authors: Sun Liying , Liu Zhenju , Zheng Mingguo , Cai Qiangguo , Fang Haiyan 
Published: December 2013

Abstract
The deterioration of ecological situation with serious soil and water loss in black soil region of northeast China has attracted more attention due to its significant role on food security of China. To investigate the temporal characteristics of ecological status in typical black soil areas, Baiquan County is selected. Based on the model of Press-Status-Response (P-S-R), indicators are established and the ecological security situations with soil and water loss of Baiquan County are evaluated for the years of 1979, 1990, 2000 and 2005. The results show that the ecological insecurity indicator changes from 0.701 to 0.435 from 1979 to 2005, with a decrease of 37.9% for Baiquan County. And the contributions of physical and human factors to the temporal variations of the ecological security are discussed in detail. Moreover, several problems are recognized to be the potential threats to the ecological security in Baiquan county, including reduction of the effective thickness, excessive application of the fertilizer and low efficiency of the agricultural irrigation system. It is found that effective soil and water loss control actions have made great contribution to the improvement of the ecological security in Baiquan county. All these results and discussions are very helpful for the further investigation on the quantitative relationship between soil and water loss and ecological security in black soil region of northeast China.
MYOD mediates skeletal myogenic differentiation of human amniotic fluid stem cells and regeneration of muscle injury
Authors: Kim Ju Ang , Shon Yun Hee , Lim Jeong Ok , Yoo James J , Shin Hong-In , Park Eui Kyun 
Published: December 2013

Abstract
Introduction
Human amniotic fluid stem (hAFS) cells have been shown to differentiate into multiple lineages, including myoblasts. However, molecular mechanisms underlying the myogenic differentiation of hAFS cells and their regenerative potential for muscle injury remain to be elucidated.

Methods
In order to induce myogenic differentiation of hAFS cells, lentiviruses for MYOD were constructed and transduced into hAFS cells. Formation of myotube-like cells was analyzed by immunocytochemistry, and expression of molecular markers for myoblasts was analyzed by reverse transcription polymerase chain reaction and Western blotting. For in vivo muscle regeneration, MYOD transduced hAFS cells were injected into left tibialis anterior (TA) muscles injured with cardiotoxin, and muscle regeneration was analyzed using hematoxylin and eosin, immunocytochemistry and formation of neuro-muscular junction.

Results
MYOD expression in hAFS cells successfully induced differentiation into multinucleated myotube-like cells. Consistently, significant expression of myogenic marker genes, such as MYOG, DES, DMD and MYH, was induced by MYOD. Analysis of pre-myogenic factors showed that expression of PAX3, MEOX1 and EYA2 was significantly increased by MYOD. MYOD was phosphorylated and localized in the nucleus. These results suggest that in hAFS cells, MYOD is phosphorylated and localized in the nucleus, thus inducing expression of myogenic factors, resulting in myogenic differentiation of hAFS cells. To test regenerative potential of MYOD-transduced hAFS cells, we transplanted them into injured muscles of immunodeficient BALB/cSlc-nu mice. The results showed a substantial increase in the volume of TA muscle injected with MYOD-hAFS cells. In addition, TA muscle tissue injected with MYOD-hAFS cells has more numbers of neuro-muscular junctions compared to controls, indicating functional restoration of muscle injury by MYOD-hAFS cells.

Conclusions
Collectively, our data suggest that transduction of hAFS cells with MYOD lentiviruses induces skeletal myogenic differentiation in vitro and morphological and functional regeneration of injured muscle in vivo.
Yeast RAD2, a homolog of human XPG, plays a key role in the regulation of the cell cycle and actin dynamics
Authors: Kang Mi-Sun , Yu Sung-Lim , Kim Ho-Yeol , Gorospe Choco Michael , Choi Byung Hyune , Lee Sung Haeng , Lee Sung-Keun 
Published: December 2013

Summary
Mutations in the human XPG gene cause Cockayne syndrome (CS) and xeroderma pigmentosum (XP). Transcription defects have been suggested as the fundamental cause of CS; however, defining CS as a transcription syndrome is inconclusive. In particular, the function of XPG in transcription has not been clearly demonstrated. Here, we provide evidence for the involvement of RAD2, the Saccharomyces cerevisiae counterpart of XPG, in cell cycle regulation and efficient actin assembly following ultraviolet irradiation. RAD2 C-terminal deletion, which resembles the XPG mutation found in XPG/CS cells, caused cell growth arrest, the cell cycle stalling, a defective α-factor response, shortened lifespan, cell polarity defect, and misregulated actin-dynamics after DNA damage. Overexpression of the C-terminal 65 amino acids of Rad2p was sufficient to induce hyper-cell polarization. In addition, RAD2 genetically interacts with TPM1 during cell polarization. These results provide insights into the role of RAD2 in post-UV irradiation cell cycle regulation and actin assembly, which may be an underlying cause of XPG/CS.
Differential expression of the FMRF gene in adult and hatchling stellate ganglia of the squid Loligo pealei
Authors: Burbach J. Peter H. , Grant Philip , Hellemons Anita J. C. G. M. , Degiorgis Joseph A. , Li Ka Wan , Pant Harish C. 
Published: December 2013

Summary
The giant fiber system of the squid Loligo pealei mediates the escape response and is an important neurobiological model. Here, we identified an abundant transcript in the stellate ganglion (SG) that encodes a FMRFamide precursor, and characterized FMRFamide and FI/LRF-amide peptides. To determine whether FMRFamide plays a role in the adult and hatchling giant fiber system, we studied the expression of the Fmrf gene and FMRFamide peptides. In stage 29 embryos and stage 30 hatchlings, Ffmr transcripts and FMRFamide peptide were low to undetectable in the SG, in contrast to groups of neurons intensely expressing the Fmrf gene in several brain lobes, including those that innervate the SG. In the adult SG the Fmrf gene was highly expressed, but the FMRFamide peptide was in low abundance. Intense staining for FMRFamide in the adult SG was confined to microneurons and fibers in the neuropil and to small fibers surrounding giant axons in stellar nerves. This shows that the Fmrf gene in the SG is strongly regulated post-hatching, and suggests that the FMRFamide precursor is incompletely processed in the adult SG. The data suggest that the SG only employs the Fmrf gene post-hatching and restricts the biosynthesis of FMRFamide, demonstrating that this peptide is not a major transmitter of the giant fiber system. This contrasts with brain lobes that engage FMRFamide embryonically as a regulatory peptide in multiple neuronal systems, including the afferent fibers that innervate the SG. The biological significance of these mechanisms may be to generate diversity within Fmrf-expressing systems in cephalopods.
Introduction of a Disulfide Bond as a Key Element of Acyclic Bis-Thiourea-Type Anion Receptors
Authors: Tomita Kentaro , Ishioka Toshio , Harata Akira 
Published: December 2013

Abstract
This article shows that a difference in the building blocks of the molecular framework, C–C to S–S, results in a significant influence on its optical properties. Acyclic anion receptors, bis{2-[3-(substituted)thioureido]ethyl} disulfides were newly synthesized, and their optical properties in response to complex formation with an acetate ion were compared with those of a hexamethylene framework between two thioureido groups. Quantum chemical calculations suggested that receptors fold their molecular framework around disulfide because of the presence of the intramolecular hydrogen bonding between two N,N′-disubstituted thioureas. The complex of the receptor and the acetate ion with 1:1 stoichiometry consists of the coordination of two thioureido groups to the acetate via hydrogen bonds. This complex formation is accompanied by a dissociation of intramolecular hydrogen bonding and rotation of one side of the thioureido group. These circumstances of conformational changes in the molecular framework functioned by switching on and off the excimer fluorescence of the 1-pyrenylmethyl derivative.
Synthesis and Properties of a Through-space-conjugated Dimer
Authors: Morisaki Yasuhiro , Kawakami Naoya , Nakano Tatsuya , Chujo Yoshiki 
Published: December 2013

Abstract
We describe the synthesis and energy-transfer characteristics of a [2.2]paracyclophane-based through-space-conjugated dimer consisting of two stacked π-electron systems. Photoexcited energy transfer by the Förster mechanism from the donor to acceptor π-electron systems occurred efficiently (energy-transter efficiency: ΦET &gt; 0.999) with a rate constant (kET) of 1012 s−1.
Improved Selective Segmentation Model Using One Level-Set
Authors: Rada Lavdie , Chen Ke 
Published: December 2013

Variational segmentation models have proven to be extremely efficient for segmenting and tracking boundaries and in most cases all of the boundaries in an image. Such models are for global segmentation. For a large class of image segmentation tasks where only one object is required to be extracted automatically, global models cannot deliver the solution and we need selective segmentation techniques. In this paper, we propose a novel, variational and single level-set function for the selective segmentation task. The model is much faster to implement than the previously dual level set model by Rada-Chen [29] by having the same efficiency and reliability. In comparing with interactive image segmentation algorithm of Nguyen-Cai-Zhang-Zheng method [2], our model shows some improvement in some cases. Several new ideas are incorporated in this new work: i) the distance function is only needed optionally and its inclusion does not affect the result; ii) an adaptive parameter is introduced in the edge detection function; iii) a new area-based fitting term is added to enhance the model's reliability (different from the idea of minimizing the area of the object). We develop an additive operator splitting method for solving the resulting Euler-Lagrange equation. Test results show that the new model finds the desired local boundaries successfully in various challenging cases and indeed it is not much dependent on the prior information of markers or the distance function based on them. More importantly, the new model gives an overall improvement over the previous models and can be recommended for selective segmentation.
Ruined Archeological Heritage. Survey, 3D Modeling, Virtual Anastylosis
Authors: Agnello Fabrizio , Cannella Mirco 
Published: December 2013

The study focuses on the use of 3D surveying and digital representation tools for the purpose of documenting archaeological monuments whose restoration involves the disassembly and repositioning of blocks. The case study in question is a building at the west end of the San Biagio archaic sanctuary, located within the archaeological site of Agrigento. The monument is a rectangular building encompassing two pools, whose foundations are suffering from deep decay and there are large spaces between the walls which have become disconnected. Surveying and 3D modelling have been used to illustrate the existing state of the building. Plans, sections and orthophotos have been created to support the indexing of ashlars and to evaluate the structural decay. A 3D model of the most dilapidated part of the building, the north-eastern corner, has been constructed in order to document the direction of the ashlars and where they are positioned in relation to each other. Finally, a restoration model of this building been built.
Regulation of Cancerous inhibitor of PP2A (CIP2A) by small molecule inhibitor for c-Jun NH2-Terminal Kinases (JNKs), SP600125, in Human Fibrosarcoma (HT1080) cells
Authors: Khanna Anchit 
Published: December 2013

Background: Protein phosphatase 2A inhibition is one of the pre-requisites for human cell transformation. Previously, we have identified an endogenous inhibitor of PP2A, CIP2A (Cancerous Inhibitor of Protein Phosphatase 2A) in human fibrosarcoma cells (HT1080) using tandem affinity purification. CIP2A over expression has been demonstrated in almost every tumour type studied so far. However, our understanding on the mechanisms regulating CIP2A expression in human cancers, especially in sarcomas, is still emerging. Methods: Human fibrosarcoma (HT1080) cells were treated with small molecule inhibitors against the three major signalling pathways, namely p38, MEK and JNK pathways to identify the pathway regulating CIP2A expression in the sarcoma cells. This was followed by verification of the results using small interfering RNAs (siRNA) for the kinases. Results: In line with previous observations, small molecule inhibitor for MEK pathway (PD98059) decreased CIP2A mRNA and protein expression. Interestingly, small molecule inhibitor for the JNK pathway, SP600125 decreased mRNA and protein levels of CIP2A oncoprotein with negligible effect of SB203580 (p38 kinase) inhibitor on CIP2A expression in HT1080 cells. However, siRNAs specific to either JNK1 or JNK2 kinases did not result in decrease in CIP2A expression. Contrarily, two different CIP2A siRNAs, which were used as positive controls, decreased JNK2 expression in HT1080 cells. Conclusion: Although it is well established that SP600125 inhibits JNK kinases, it has also been shown to inhibit a spectra of other kinases. SP600125 inhibits CIP2A protein expression both in time and concentration dependent manner. However, depletion of both JNK1 and JNK2 kinases using specific siRNAs fails to decrease CIP2A protein expression levels, thereby indicating the need to verify the results obtained by treatment with small molecular inhibitors of kinases by independent approaches like two different target specific siRNAs. Finally, fortuitously we identify JNK2 as a CIP2A downstream target in HT1080 cells.
MANAGEMENT SCHEME OF OPERATIONAL RISKS IN A BASIS SYSTEM OF RISK MANAGEMENT
Authors: Соколов Дмитрий , Sokolov Dmitriy , Барчуков Алексей , Barchukov Alyeksyey 
Published: December 2013

A basis system of risk management described in the article
can be adapted for business activity special aspects of organization’s
territorial and structural business units for construction of private
systems of management. The authors offer Management scheme of
operational risks.
APPROACHES TO THE FORMATION OF BALANCED SCORECARD LOGISTICS ACTIVITY OF THE ENTERPRISE
Authors: Саплина Татьяна , Saplina Tatyana , Курлыкова Анна , Kurlykova Anna 
Published: December 2013

Review and critical analysis of approaches to the formation
of balanced scorecard of logistics activity have been presented in the
article. The algorithm of choice of balanced scorecard logistics activity
of the enterprise has been offered.
An action research project on the use of Facebook in an undergraduate visual communication study unit
Authors: Souleles Nicos 
Published: December 2013

Abstract
This article describes a technical action research project on how Facebook can be embedded in the structure of an undergraduate visual communication study unit. It identifies educational benefits as well as pitfalls, and how students perceive this Social Networking Site (SNS) as part of their teaching and learning process. Data was collected through an online questionnaire, content analysis of Facebook posts and a focus group. The limitations of this investigation relate to the generalizability of findings that are the result of action research. Students identified a range of educational benefits relating to peer-to-peer but also teacher-student support. Among the challenges were student attitudes and perceptions of what should or should not occur with the Facebook group when used for teaching and learning. This article provides a blueprint for the next cycle of the action research process, which can include specific refinements, but also needs to consider a number of challenges.
An instructional design for Building Information Modeling (BIM) and Revit in interior design curriculum
Authors: Asojo Abimbola O. 
Published: December 2013

Abstract
Current trends in integrated project design and delivery methods demand the development of new software competencies in interior design pedagogy. Building Information Modeling (BIM) has revolutionized visualization, preconstruction simulation, life cycle analysis, and enabled faster construction, thus promoting more sustainable integrated practices with a rich repertoire of building information available for the design team. This new tool presents opportunities for interior design educators to teach software and skills that are necessary to prepare students for integrated practice. This article discusses an instructional design approach of introducing BIM and Revit into the interior design curriculum at University of Minnesota. John Robert Anderson’s ACT-R (Adaptive Control of Thought-Rational) theory is utilized to guide students through the learning process. ACT-R theory focuses on three stages of skill acquisition: cognitive, associative and autonomous stages. These three stages present implications for teaching interior design students about BIM and Revit.
Revolt into style: Consumption and its (dis)contents in Valery Todorovsky’s film Stilyagi
Authors: Roberts Graham H. 
Published: December 2013

Abstract
A ‘rites of passage’ movie set in the USSR of the 1950s, Valery Todorovsky’s film Stilyagi (2008) tells the story of the transformation of the hero Mels from loyal member of the Komsomol to stilyaga. For many critics, Todorovsky’s stilyagi are the very embodiment of anti-conformism. And yet to read the film in this way is to overlook one of its key elements – consumption. For in order to acquire their clothes and other accessories, the stilyagi need money. Zooming in (literally) on the financial transactions that these young people engage in with such astonishing regularity, Todorovsky reminds us that without money, there can be no ‘style’, and consequently, no stilyagi. Seen in this light, the stilyagi are not at all subversive, but in fact highly conformist precursors of today’s post-soviet shoppers. It is this paradox at the heart of the stilyagi and their ‘revolt’ as presented by Todorovsky, which will be the subject of our article.