bluuebunny/crossref_metadata_2025_split huggingface dataset:
Measuring deoxygenation effects on marine predators: A new animal‐attached archival tag recording in situ dissolved oxygen, temperature, fine‐scale movements and behaviour
Authors: da Costa Ivo , Sims David W. , Loureiro Bruno , Waller Matt J. , Womersley Freya C. , Loveridge Alexandra , Humphries Nicolas E. , Southall Emily J. , Vedor Marisa , Mucientes Gonzalo , Prendergast Sophie , Fontes Jorge , Afonso Pedro , Macena Bruno C. L. , Watanabe Yuuki Y. , Queiroz Nuno 
Published: July 2024

Abstract

Global climate‐driven ocean warming has decreased dissolved oxygen (DO) levels (ocean deoxygenation) leading to expansions of hypoxic zones, which will affect the movements, behaviour, physiology and distributions of marine animals. However, the precise responses of animals to low DO remains poorly understood because movements and activity levels are seldom recorded alongside instantaneous DO in situ.

We describe a new animal‐attached (dissolved oxygen measuring, DOME) archival tag with an optical oxygen sensor for recording DO, in addition to sensors for temperature and depth, a triaxial accelerometer for fine‐scale movements and activity, and a GPS for tag recovery. All sensors were integrated on a single electronic board.

Calibration tests demonstrated small mean difference between DOME tag and factory‐calibrated DO sensors (mean relative error of 5%). No temporal drift occurred over a test period three times longer than the maximum deployment time. Deployments on four blue sharks (Prionace glauca) in the central North Atlantic Ocean showed regular vertical oscillations from the surface to a maximum of 404 m. Profiles from diving sharks recorded DO concentrations ranging from 217 to 272 μmol L−1, temperatures between 13°C and 23°C, and identified an oxygen maximum at ~45 m depth, all of which were consistent with ship‐based measurements. Interestingly, the percentage of time sharks spent burst swimming was greater in the top 85 m compared to deeper depths, potentially because of higher prey availability in the surface layer.

The DOME tag described blue shark fine‐scale movements and activity levels in relation to accurately measured in situ DO and temperature, with the potential to offer new insights of animal performance in low oxygen environments. Development of a tag with physico‐chemical and movement sensors on a single electronic board is a first step towards satellite relay of these data over broader spatiotemporal scales (months over thousands of kilometres) to determine direct and indirect responses of marine animals to heatwave and deoxygenation events.
Longitudinal associations of retinal vessel morphology with intraocular pressure and blood pressure at follow‐up visit—Findings from a Danish eye and vision cohort, Project FOREVER
Authors: Freiberg Josefine , Welikala Roshan , Rovelt Jens , Barman Sarah A. , Owen Christopher G. , Rudnicka Alicja R. , Kolko Miriam ,   the FOREVER consortium
Published: July 2024

AbstractPurposeTo characterise the retinal vasculometry of a Danish eye and vision cohort and examine associations with systolic blood pressure (BP), diastolic BP, mean arterial BP, and intraocular pressure (IOP).DesignLongitudinal study.MethodsThe retinal vasculature of fundus images from the FOREVER (Finding Ophthalmic Risks and Evaluating the Value of Eye exams and their predictive Reliability) cohort was analysed using a fully automated image analysis program. Longitudinal associations of retinal vessel morphology at follow‐up visit with IOP (baseline and follow‐up) and BP (follow‐up) were examined using multilevel linear regression models adjusting for age, sex and retinal vasculometry at baseline as fixed effects and person as random effect. Width measurements were additionally adjusted for the spherical equivalent.ResultsA total of 2089 subjects (62% female) with a mean age of 61 (standard deviation 8) years and a mean follow‐up period of 4.1 years (SD 0.6 years) were included. The mean arteriolar diameter was approximately 20% thinner than the mean venular diameter, and venules were about 21%–23% less tortuous than arterioles. BP at follow‐up was associated with decreased arteriolar diameter from baseline to follow‐up. After adjusting for baseline IOP, IOP at follow‐up was associated with increased arteriolar tortuosity above baseline (0.59%, 95% CI 0.08–1.10, p‐value 0.024).ConclusionIn a Danish eye and vision cohort, variations in BP and alterations in IOP over time were associated with changes in the width and tortuosity of retinal vessels. Our findings contribute novel insights into retinal vascular alterations over time.
New surgery technique combining robotics and laparoscopy using the Hugo™ RAS system
Authors: Komatsu Hiroaki , Sawada Mayumi , Iida Yuki , Wada Ikumi , Azuma Yukihiro , Kudoh Akiko , Sato Shinya , Harada Tasuku , Taniguchi Fuminori 
Published: July 2024

AbstractIntroductionHybrid total laparoscopic hysterectomy combines conventional laparoscopic surgery and robot‐assisted devices: the camera and assistant forceps are operated by a robotic device, whereas the surgeon performs laparoscopic procedures, enabling surgery with a completely fixed field of view and significantly reducing errors in forceps grasping and needle misalignment. Here, we examined whether using two arms of the Hugo™ robot‐assisted surgery system, one for the camera and one for the assistant, would improve surgical accuracy compared with conventional total laparoscopic hysterectomy.Materials and Surgical TechniqueThe surgical system reduced surgeon errors in grasping the forceps during training and stabilized forceps operation. Compared with conventional laparoscopic surgery, the use of the surgical system did not result in different operative durations. The stable surgical procedure was considered a major advantage.DiscussionThis new technique involving new equipment can improve surgeon training and performance. In the future, we will develop new techniques to improve surgical performance.
Comparing grass biomass estimation methods for management decisions in a semi‐arid landscape
Authors: Riquelme Linda , Rumpff Libby , Duncan David H. , Vesk Peter A. 
Published: July 2024

AbstractAimsEnvironmental managers require reliable and cost‐efficient monitoring methods for effective decision‐making. Understanding forage availability is important for managing wild, vertebrate herbivore populations. We developed a process for exploring the accuracy and cost efficiency of various biomass estimation techniques for a case study where semi‐arid woodland restoration is threatened by kangaroo grazing, with the aim of determining which method was most fit for purpose in a given decision context.LocationWyperfeld National Park, southeastern Australia.MethodsGrass biomass was estimated using a variety of methods, then compared to clipped biomass using linear models. Biomass estimation methods were either field‐based (i.e., rising plate meter, multispectral radiometer) or satellite‐based (i.e., Landsat satellite imagery, AussieGRASS forage production model). Sampling occurred across open and wooded semi‐arid vegetation types. We compared methods based on accuracy, the ability of each method to accurately predict a ‘forage‐switch’ threshold, cost, and the suitability for the management context.ResultsFor this case study, the multispectral radiometer was the most precise, yet most expensive, biomass estimation method over a single survey. However, satellite imagery proved to be the most cost‐efficient and fit for purpose, as it was inexpensive and most accurately estimated biomass around a forage‐switch threshold, second only to the multispectral radiometer. Accuracy of all methods was improved by including tree cover in the regression models.ConclusionsWe demonstrate a process for exploring which biomass estimation tool might be preferred for a given decision context, highlighting accuracy, consideration of tolerance to uncertainty and risk, the spatial and temporal scale of information required, and budget constraints.
Late syn‐rift to early post‐rift basin fill dynamics of a mixed siliciclastic‐carbonate succession banked to a basement high, Hornsund, southwestern Spitsbergen, Arctic Norway
Authors: Dahlin Anders , Blinkenberg Kasper H. , Braathen Alvar , Olaussen Snorre , Senger Kim , Smyrak‐Sikora Aleksandra , Stemmerik Lars 
Published: July 2024

AbstractThe transition from syn‐rift to post‐rift sedimentation in rift basins is difficult to characterize in terms of stratigraphic architecture and dominating control on sedimentation, due to decreasing tectonic activity interplaying with regional subsidence, eustatic sea level changes, and differential compaction of underlying syn‐rift sediments. Our case study of the Late Palaeozoic Inner Hornsund Fault Zone targets late syn‐rift strata recorded in the (?Pennsylvanian – ?lower Permian) Treskelodden Formation in Hornsund, southern Spitsbergen, representing a mixed siliciclastic‐carbonate succession, with siliciclastics primarily sourced from the adjacent Sørkapp‐Hornsund High. We document local scale (<10 km) facies variability, sequence stratigraphy, and evolution of a succession deposited along a flank of the structural high during the late syn‐rift stage. We observe that during the transition towards rift termination (glacio‐)eustatic sea level changes and overall regional flooding became a more prominent forcing factor controlling sedimentation. Our dataset includes sedimentary logs, microfacies analysis, and high‐resolution digital outcrop models. We identify four progressively backstepping stratigraphic sequences, reflecting an evolution from (1) terrestrial siliciclastics through (2–3) nearshore mixed siliciclastic–carbonates, to (4) carbonate ramp deposits. On the small scale (<5 m) the internal sediment cyclicity of the succession was formed by autogenic processes, particularly the changing rate of sediment input from the southwestern source area (the uplifted Sørkapp‐Hornsund basement high). On the larger scale (10s of m), the importance of glacio‐eustatic sea‐level changes, driven by waxing and waning of ice caps in the southern hemisphere (Gondwana), increased as the rift‐related tectonics decreased. The interdisciplinary methods used in this study provide new knowledge of the Middle Pennsylvanian to Permian depositional evolution in southern Spitsbergen, besides a novel framework for comparison to adjacent basins in the region and similar basins elsewhere.
The bidirectional relationship between peer relationships and bullying: Evidence from cross‐lagged analyses among Chinese children
Authors: Li Qing , Chu Xiaowei , Yang Yuxin , Jia Yunzhen 
Published: July 2024

AbstractBackgroundIn the digital age, bullying manifests in two distinct forms: traditional bullying and cyberbullying. Children's peer relationships are important predictors of bullying, and bullying in turn predicts peer relationships. However, few researchers have noted the bidirectional relationship between peer relationships and bullying.MethodsThe present study used a two‐wave cross‐lagged longitudinal design to fill this gap. The potential sex differences were also examined in this relationship. The sample consisted of 527 Chinese children aged 8 to 12 years (M = 9.69, SD = .96; 53.5% female). Participants completed peer nominations for peer acceptance, peer rejection and social dominance, as well as self‐reports of traditional bullying and cyberbullying.ResultsResults showed that peer rejection at the first time point (T1) significantly and positively predicted traditional bullying perpetration, cyberbullying perpetration and cyberbullying victimization at the second time point (T2). Traditional bullying victimization at T1 significantly and negatively predicted peer acceptance and social dominance at T2. The results also revealed significant male and female differences. For instance, among boys, peer acceptance at T1 significantly and negatively predicted cyberbullying victimization at T2. In contrast, this relationship was not observed among girls. The present findings have important implications for understanding the cyclical relationship between peer relationships and bullying and providing practical guidance for improving peer relationships and reducing bullying.
Low‐intensity noise exposure takes an essential part in the mechanism of late‐onset hereditary hearing loss caused by Abcc1 mutation
Authors: Jiang Mengzhu , Zhang Shuai , Liu Jing , Pang Bo , Bai Yijiang , Chen Yongjia , Huang Huping , Ling Jie , Mei Lingyun 
Published: July 2024

AbstractWith the development of the social economy, we are exposed to increasing noise in our daily lives. Our previous work found an ABCC1(NM_004996.3:c.A1769G, NP_004987.2:p.N590S) variant which cosegregated with the patients in an autosomal dominant non‐syndromic hearing loss family. At present, the specific mechanism of deafness caused by ABCC1 mutation is still not clear. Using the knock‐in mouse model simulating human ABCC1 mutation, we found that the occurrence of family‐related phenotypes was likely attributed to the combination of the mouse genotype and low‐intensity noise. GSH and GSSG are important physiological substrates of ABCC1. The destruction of GSH‐GSSG balance in the cochleae of both Abcc1N591S/+ mice and Abcc1N591S/N591S mice during low‐intensity noise exposure may result in irreversible damage to the hair cells of the cochleae, consequently leading to hearing loss in mice. The findings offered a potential novel idea for the prevention and management of hereditary hearing loss within this family.
RNA m6A modification regulates cell fate transition between pluripotent stem cells and 2‐cell‐like cells
Authors: Su Zhongqu , Dong Yu , Sun Jiatong , Wu You , Wei Qingqing , Liang Yuwei , Lin Zhiyi , Li Yujun , Shen Lu , Xi Chenxiang , Wu Li , Xu Yiliang , Liu Yingdong , Yin Jiqing , Wang Hong , Shi Kerong , Le Rongrong , Gao Shaorong , Xu Xiaocui 
Published: July 2024

AbstractN6‐methyladenosine (m6A) exerts essential roles in early embryos, especially in the maternal‐to‐zygotic transition stage. However, the landscape and roles of RNA m6A modification during the transition between pluripotent stem cells and 2‐cell‐like (2C‐like) cells remain elusive. Here, we utilised ultralow‐input RNA m6A immunoprecipitation to depict the dynamic picture of transcriptome‐wide m6A modifications during 2C‐like transitions. We found that RNA m6A modification was preferentially enriched in zygotic genome activation (ZGA) transcripts and MERVL with high expression levels in 2C‐like cells. During the exit of the 2C‐like state, m6A facilitated the silencing of ZGA genes and MERVL. Notably, inhibition of m6A methyltransferase METTL3 and m6A reader protein IGF2BP2 is capable of significantly delaying 2C‐like state exit and expanding 2C‐like cells population. Together, our study reveals the critical roles of RNA m6A modification in the transition between 2C‐like and pluripotent states, facilitating the study of totipotency and cell fate decision in the future.
Integration of individual preclinical and clinical anti‐infective PKPD data to predict clinical study outcomes
Authors: Aranzana‐Climent Vincent , van Os Wisse , Nutman Amir , Lellouche Jonathan , Dishon‐Benattar Yael , Rakovitsky Nadya , Daikos George L. , Skiada Anna , Pavleas Ioannis , Durante‐Mangoni Emanuele , Theuretzbacher Ursula , Paul Mical , Carmeli Yehuda , Friberg Lena E. 
Published: July 2024

AbstractThe AIDA randomized clinical trial found no significant difference in clinical failure or survival between colistin monotherapy and colistin–meropenem combination therapy in carbapenem‐resistant Gram‐negative infections. The aim of this reverse translational study was to integrate all individual preclinical and clinical pharmacokinetic–pharmacodynamic (PKPD) data from the AIDA trial in a pharmacometric framework to explore whether individualized predictions of bacterial burden were associated with the trial outcomes. The compiled dataset included for each of the 207 patients was (i) information on the infecting Acinetobacter baumannii isolate (minimum inhibitory concentration, checkerboard assay data, and fitness in a murine model), (ii) colistin plasma concentrations and colistin and meropenem dosing history, and (iii) disease scores and demographics. The individual information was integrated into PKPD models, and the predicted change in bacterial count at 24 h for each patient, as well as patient characteristics, was correlated with clinical outcomes using logistic regression. The in vivo fitness was the most important factor for change in bacterial count. A model‐predicted growth at 24 h of ≥2‐log10 (164/207) correlated positively with clinical failure (adjusted odds ratio, aOR = 2.01). The aOR for one unit increase of other significant predictors were 1.24 for SOFA score, 1.19 for Charlson comorbidity index, and 1.01 for age. This study exemplifies how preclinical and clinical anti‐infective PKPD data can be integrated through pharmacodynamic modeling and identify patient‐ and pathogen‐specific factors related to clinical outcomes – an approach that may improve understanding of study outcomes.
Human primary cells can tell body time: Dedicated to Steven A. Brown
Authors: Katsioudi Georgia , Biancolin Andrew D. , Jiménez‐Sanchez Cecilia , Dibner Charna 
Published: July 2024

AbstractThe field of chronobiology has advanced significantly since ancient observations of natural rhythms. The intricate molecular architecture of circadian clocks, their hierarchical organization within the mammalian body, and their pivotal roles in organ physiology highlight the complexity and significance of these internal timekeeping mechanisms. In humans, circadian phenotypes exhibit considerable variability among individuals and throughout the individual's lifespan. A fundamental challenge in mechanistic studies of human chronobiology arises from the difficulty of conducting serial sampling from most organs. The concept of studying circadian clocks in vitro relies on the groundbreaking discovery by Ueli Schibler and colleagues that nearly every cell in the body harbours autonomous molecular oscillators. The advent of circadian bioluminescent reporters has provided a new perspective for this approach, enabling high‐resolution continuous measurements of cell‐autonomous clocks in cultured cells, following in vitro synchronization pulse. The work by Steven A. Brown has provided compelling evidence that clock characteristics assessed in primary mouse and human skin fibroblasts cultured in vitro represent a reliable estimation of internal clock properties in vivo. The in vitro approach for studying molecular human clocks in cultured explants and primary cells, pioneered by Steve Brown, represents an invaluable tool for assessing inter‐individual differences in circadian characteristics alongside comprehensive genetic, biochemical and functional analyses. In a broader context, this reliable and minimally invasive approach offers a unique perspective for unravelling the functional inputs and outputs of oscillators operative in nearly any human tissue in physiological contexts and across various pathologies.
Determinants of adherence to post‐stroke/transient ischemic attack secondary prevention medications: A cohort study
Authors: Hoarau Damien , Ramos Inès , Termoz Anne , Fernandez Violaine , Rambure Marie , Allemann Samuel S. , Derex Laurent , Haesebaert Julie , Schott Anne‐Marie , Viprey Marie 
Published: July 2024

AbstractBackgroundAdherence to post‐stroke secondary prevention medications mitigates recurrence risk. This study aimed to measure adherence to secondary prevention medications during 3 years post‐ischemic stroke/transient ischemic attack, using prescription and dispensing data, and identify factors associated with suboptimal adherence.MethodsThis multicenter, prospective, cohort study involved patients from the STROKE 69 cohort, which included all consecutive patients with suspected acute stroke admitted between November 2015 and December 2016 to any emergency department or stroke center in the Rhône area in France. Prescription data for antihypertensive agents, antidiabetic agents, lipid‐lowering drugs, and antithrombotics were collected. Dispensing data were provided by the French regional reimbursement database. Adherence was calculated using the continuous medication acquisition index. Associations between suboptimal adherence and potential influencing factors across the World Health Organization's five dimensions were explored through univariate and multivariate analyses.ResultsFrom 1512 eligible patients, 365 were included. Optimal adherence to overall treatment (≥90%) was observed in 61%, 62%, and 65% of patients in the first, second, and third years, respectively. Education level (high school diploma or higher: OR = 3.24, 95% CI [1.49; 7.36]) and depression (Hospital Anxiety and Depression Scale–Depression scores 8–10: OR = 1.90, 95% CI [1.05; 3.44]) were significantly associated with suboptimal adherence.ConclusionsOverall adherence to secondary prevention medications was fairly good. Having an initial diagnosis of transient ischemic attack, a high level of education, or depression was associated with increased odds of suboptimal adherence, while having a history of heart rhythm disorder was associated with lower odds.
Energy characteristics and damage constitutive model of pre‐cracked granite with or without filling materials
Authors: Tan Tao , Zhang Chunyang , Dai Bibo , Wang Yixian 
Published: July 2024

AbstractIn order to study the effects of pre‐crack inclination angle and filling material on granite, uniaxial compression tests were performed on pre‐cracked granite specimens without filling material and specimens filled with epoxy resin, gypsum, and organosilicon. Results indicate that the peak strength and apparent stiffness of pre‐cracked granite without filling materials increase with increasing pre‐crack inclination angle. The strain energy absorbed by the filling material improves the energy storage limit, inhibits the energy release effect when microcracks are connected to each other, and ultimately improves the compressive resistance and energy storage capacity. The strengthening effect of gypsum and epoxy resin is relatively better, which is more significant when the pre‐crack inclination angle equals 0°–60°. Finally, a damage constitutive model is established, which is in good agreement with the test and numerical simulation data. The results can provide reference for the research of grouting reinforcement of rock masses.