bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dissociation between sublingual and gut microcirculation in the response to a fluid challenge in postoperative patients with abdominal sepsis
Authors: Edul Vanina Siham Kanoore , Ince Can , Navarro Noelia , Previgliano Luciana , Risso-Vazquez Alejandro , Rubatto Paolo Nahuel , Dubin Arnaldo 
Published: December 2014

Abstract

Background
This study was performed to compare intestinal and sublingual microcirculation and their response to a fluid challenge.

Methods
Twenty-two septic patients in the first postoperative day of an intestinal surgery, in which an ostomy had been constructed, were evaluated both before and 20 min after a challenge of 10 mL/kg of 6% hydroxyethylstarch 130/0.4. We measured systemic hemodynamics and sublingual and intestinal microcirculation. Correlations between variables were determined through the Pearson test.

Results
Fluid administration increased the cardiac index (2.6 ± 0.5 vs. 3.3 ± 1.0 L/min/m2, P < 0.01) and mean arterial blood pressure (68 ± 11 vs. 82 ± 12 mm Hg, P < 0.0001). The sublingual but not the intestinal red blood cell (RBC) velocity increased (912 ± 270 vs. 1,064 ± 200 μm/s, P < 0.002 and 679 ± 379 vs. 747 ± 419 μm/s, P = 0.12, respectively). The sublingual and intestinal perfused vascular density (PVD) did not change significantly (15.2 ± 2.9 vs. 16.1 ± 1.2 mm/mm2 and 12.3 ± 6.7 vs. 13.0 ± 6.7 mm/mm2). We found no correlation between the basal sublingual and intestinal RBC velocities or between their changes in response to the fluid challenge. The individual changes in sublingual RBC velocity correlated with those in cardiac index and basal RBC velocity. Individual changes in intestinal RBC velocity did not correlate with either the cardiac index modifications or the basal RBC velocity. The same pattern was observed with the sublingual and the intestinal PVDs. The sublingual RBC velocities and PVDs were similar between survivors and nonsurvivors. But the intestinal RBC velocities and PVDs were lower in nonsurvivors.

Conclusions
In this series of postoperative septic patients, we found a dissociation between sublingual and intestinal microcirculation. The improvement in the sublingual microcirculation after fluid challenge was dependent on the basal state and the increase in cardiac output. In contrast, the intestinal microcirculation behaved as an isolated territory.
Molecular cloning and characterization of a thioredoxin from Taiwanofungus camphorata
Authors: Chen Yu-Ting , Hong Pin-Feng , Wen Lisa , Lin Chi-Tsai 
Published: December 2014

Abstract

Background
Thioredoxin (Trx) is reduced by thioredoxin reductase. Trx is used in ribonucleoide reduction, assimilatory sulfate reduction, in modulation of protein sulfhydryl groups, and refolding proteins.

Results
A TcTrx (Tc: Taiwanofungus camphorata) cDNA (640 bp, GenBank AY838902.1) encoding a putative thioredoxin (Trx) of 135 amino acid residues with calculated molecular mass of 16.17 kDa was cloned from Taiwanofungus

c

amphorata. The deduced amino acid sequence containing a motif (Cys-Gly-Pro-Cys) that is highly conserved among the reported Trxs. A three dimensional structural model of the TcTrx has been created based on the known structure of Malassezia sympodialis Trx (MsTrx, PDB ID: 2j23). To characterize the TcTrx, the codon optimized coding region was subcloned into an expression vector and transformed into Saccharomyces cerevisiae. The recombinant His8-tagged TcTrx was expressed and purified by Ni affinity chromatography. The purified enzyme showed a band of approximately 32 kDa (expected dimeric form) on a 12% SDS-PAGE. The molecular mass determined by MALDI-TOF is 33.16 kDa which suggests that the purified enzyme is a dimeric enzyme. Furthermore, the enzyme exhibited TcTrx activity via insulin assay. The Michaelis constant (K

M
) value for insulin was 3.78 × 10−2 mM. The enzyme’s half-life of deactivation was 13 min at 45°C. The enzyme was most active at pH 7.

Conclusions
A three dimensional structural model of T. camphorata Trx based on its TcTrx cDNA sequence. The active form of the TcTrx has been successfully expressed in yeast. The enzyme possesses Trx activity and is capable of reduction of disulfide bonds during the formation of newly synthesized proteins.
Features of the spatial organization of services in Dagestan (based on hotel and restaurant businesses)
Authors: Гусенова Джамилия , Gusenova Dzhamiliya 
Published: December 2014

The article examines some of the features of service in the Republic of Dagestan. The paper provides a specific statistical evidence of uneven development of the service sector in the region, including a faster growth in the average monthly wage in some areas of the service sector. In particular, there is a relatively lower level of demand for jobs among service workers, workers in housing and utilities, trade and related activities (5.3% of total demand) whereas for those professions there is an above-average wage growth in comparison to the whole economy. Thus, the average monthly wage in the hotel and restaurant sector in January-May 2014 compared to the same period in 2013 was 134.1%, which is almost 23%&#62; higher than the average salary increase for the whole economy.
Particular attention is paid to the spatial organization of catering in Makhachkala. As a result of cartographic data placement of objects and their subsequent analysis identified are 6 specific territorial zones of the urban environment in which the great bulk of catering is concentrated. The author assumes the existence of a positive relation between the placement of enterprises and the location of the major higher educational institutions of the city, which indicates the orientation of catering on the student youth as the main consumer of goods and services. The methodological basis of the research is comprised of general scientific methods and particular studies of the phenomenon of «service area». Comparative analysis as a scientific method has allowed revealing the features of the organization of the service area, relative to the other spheres of economic activity. Among other methods actively is used content analysis of Internet resources, involving quantitative calculation of the control units (keywords).
Immediate changes in pressure pain threshold in the iliotibial band using a myofascial (foam) roller
Authors: Vaughan Brett , McLaughlin Patrick , Lepley Adam S 
Published: December 2014

Background Foam rolling is a self-release myofascial technique that can be applied to a variety of tissues. Previous studies have demonstrated physiological changes in range of motion and arterial stiffness with the application of a foam roller; however, no studies have investigated the change in pain levels. The present study investigated the effect on the pressure pain threshold (PPT) following the application of a foam roller for three minutes to the right iliotibial band (ITB) of asymptomatic participants. Methods Participants completed a questionnaire and had their height and mass measured. Three points on the ITB of the right leg were marked. The PPT was measured at each point using a pressure algometer. Measurements were taken pre-intervention, post-intervention and five minutes post-intervention. Participants completed a single three-minute bout on the foam roller. Findings Results demonstrated a statistically significant increase (p<0.05) in the PPT at the lower thigh immediately post-treatment; however, the difference was ameliorated five minutes later. Conclusions Foam rolling the ITB produces an immediate increase in the PPT of the lower thigh in asymptomatic participants.
An observational study on usual physiotherapy care in a stroke rehabilitation unit for patients with mobility deficits
Authors: Tole Genevieve , Williams Gavin , Clark Ross , Holland Anne E , Jackson Joanna 
Published: December 2014

Aim To document the range of physiotherapy interventions undertaken by stroke patients with mobility deficits during ‘usual care’ in inpatient rehabilitation. Methods Twenty consecutively admitted adults were approached to participate if they met the inclusion criteria: diagnosis of first stroke with leg weakness; functional ambulation category score ≥ able to mobilise ≥14 metres on level ground; and able to provide consent. All individual physiotherapy sessions for each participant were observed for one day. The duration of each therapy session was recorded, as well as the duration and type of specific physiotherapy interventions. Findings Twenty participants (12 males, 8 females; mean±SD age: 69±20 years) were observed. All participants attended at least one individual session of physiotherapy on the day this study was conducted. Physiotherapy sessions lasted 47.8±7.6 minutes, with an average of 17.9±6.9 minutes spent resting. The most frequently provided therapies were: gait training (10.8±4.7 minutes per session); strength training (8.5±7.0 minutes per session); and balance training (6.2±3.6 minutes per session). Conclusions In this sample of ambulant patients following stroke, inpatient physiotherapy rehabilitation focused on gait training, lower limb strengthening and balance training. Active therapy time was low and long rest periods with no prescribed activity were evident, which suggests therapy time is not optimally utilised in this population.
Developing Electronic Data Methods Infrastructure to Participate in Collaborative
Research Networks
Authors: Priest Elisa L. , Klekar Christopher , Cantu Gabriela , Berryman Candice , Garinger Gina , Hall Lauren , Kouznetsova Maria , Kudyakov Rustam , Masica Andrew 
Published: December 2014

Context: Collaborative networks support the goals of a learning health system by
sharing, aggregating, and analyzing data to facilitate identification of best practices
care across delivery organizations. This case study describes the infrastructure and
process developed by an integrated health delivery system to successfully prepare and
submit a complex data set to a large national collaborative network.Case Description: We
submitted four years of data for a diverse population of patients in specific clinical
areas: diabetes, chronic heart failure, sepsis, and hip, knee, and spine. The most
recent submission included 19 tables, more than 376,000 unique patients, and almost 5
million patient encounters. Data was extracted from multiple clinical and administrative
systems.Lessons Learned: We found that a structured process with documentation was key
to maintaining communication, timelines, and quality in a large-scale data submission to
a national collaborative network. The three key components of this process were the
experienced project team, documentation, and communication. We used a formal QA and
feedback process to track and review data. Overall, the data submission was resource
intensive and required an incremental approach to data quality.Conclusion: Participation
in collaborative networks can be time and resource intense, however it can serve as a
catalyst to increase the technical data available to the learning health
system.
Underscore Alchemy: Extending the Underscore for creative artists
Authors: McKenzie Vahri 
Published: December 2014

Abstract
Underscore Alchemy is a research project designed to investigate how artists from disciplines outside the performing arts might engage with Nancy Stark Smith’s collaborative improvisation model, the Underscore, in order to enhance their creative practices. Creative artists with limited prior experience of somatic practices were invited to participate in a series of research workshops based on Smith’s Underscore. These participants were offered somatic exercises based on everyday movements by facilitators trained in contact and improvisation, within an egalitarian workshop culture. This phenomenological study captured data generated through practice as well as qualitative data, analysis of which suggests that the Underscore can be successfully adapted for artists working in non-dance disciplines. This article looks at the role Smith’s Underscore played in Underscore Alchemy, entailing an investigation of how contact manifested through rhythmic and visual patterns, as well as how these creative artists from a variety of disciplines engaged with improvisation through attention to the experiential body.
touch + talk: Ecologies of questioning in contact and improvisation
Authors: Sarco-Thomas Malaika 
Published: December 2014

Abstract
This article explores the potential of the touch + talk score to act as a framework in which to articulate and open up dance practitioners’ questions underlying the practice and performance of contact and improvisation. Guattari’s model of three ecologies is used as a reference point from which to identify how a practice of questioning is significant to improvisational modalities in dance as well as wider practices of relating to social, mental and physical spheres of thought. I suggest that negotiating contact with another through physical and verbal questioning can be a route towards articulating our emerging understanding of current improvisation practices and makes visible the ethical practice of encountering another.
Risk and flow in contact improvisation: Pleasure, play and presence
Authors: Urmston Elsa , Hewison James 
Published: December 2014

Abstract
Grounded in Csikszentmihalyi’s flow theory, the focus of this study was to examine the role of flow as a framework for optimizing the practice of risk-taking in the teaching and learning of contact improvisation in higher education, and to better enable students to take the creative, physical and psychological risks associated with that practice. A range of qualitative methodologies were employed including observation, analysis, writing, drawing and focus group discussions. Findings of this study included: establishing shared learning environments, attending to language and tone of delivery, and ensuring the development of scaffolded tasks for trust, non-judgement, optimal experience and pleasure to flourish.
A pedagogy of vital contact
Authors: Smith Stephen J. 
Published: December 2014

Abstract
Children, youth, other persons, animals even are those with whom our lives are entwined, yet our connections are customarily superficial, fleeting and distant. Where they are sustained in various professional and pedagogical practices, the relations tend to be constrained by routines, rituals and rites. The primary motive of contact improvisation, however, is to connect to others beyond our habitual containments. As a movement form, contact improvisation brings postural, positional, gestural and expressive nuance to our actions, reactions and interactions. It is a somatic practice not only of relating sensitively to others, but also of cultivating a corporeal responsiveness to those who would otherwise fall outside our personal and professional commitments. We realize the possibilities of such connection when the spatial, and especially the temporal, dynamics of contact improvisation are transposed ‘somatophorically’ to discursive realms. A felt durational emphasis can be now heard as the agogic accent of multilingualism. And as an accent of speech, but also of song, music and motion, its significance spreads through all our relations with others. Particular attention is paid to the pedagogical formulation of this accent and to how improvisatory contact might be transposed to settings of evident educational concern. Relations between teachers and students, therapists and clients, social workers and youth, as well as parents and their children, can incorporate this accent of contact duration. At their pedagogical best, these relations can be corporeally improvisatory, multilingually responsive, and essentially and durationally tactful. More formal, institutionalized representations of pedagogy can thus be understood as derivative, and too often subversive, of a vital contact with others in the manifold, multilingual worlds we share.
Fatal Snake Bite in a Brown Bear (Ursus Arctos L.): A Case Report
Authors: Velev Romel , Tankoski Toni , Tankoska Maja 
Published: December 2014

Abstract
Poisoning from snake venom in animals is an emergency which requires immediate attention or otherwise, the delayed and inadequate treatment leads to untoward consequences and death. The present paper describes a case of venomous snakebite in a brown bear cub (Ursus arctos L.) and its therapeutic management. The brown bear cub of which was found alone on the slopes of a mountain in the southwest part of the country was presented to the peripheral veterinary practice in Ohrid with a history of dullness, disorientation and excessive swelling around the left forepaw. It was diagnosed for snakebite based on the history and physical examination. The hematological parameters showed reduced values of hemoglobin, packed cell volume and increased total leukocyte count. The biochemical values showed elevated levels of alanine aminotransferase and creatinine. After immobilization of the animal, the treatment was conducted with fluids, corticosteroid and broad spectrum antibiotic with careful monitoring. Despite the treatment which was initiated immediately, it was only partially effective, and the animal died one hour after the beginning of its course. Poisonous snakes are common in the mountainous part of Macedonia and, just like humans, wild bears especially their cubs are susceptible to the deadly venom of some species. The severity of the reaction to snake venom and prognosis in animals depends on a number of factors: on the type and species of snake, on how much venom was injected, on the location of the bite, on the age, health and body weight of the animal and crucially, the time interval between the snakebite and the application of the treatment.
Music-induced prolongation of heart rate corrected QT intervals from electrocardiogram recordings of healthy preterm pregnant women
Authors: Rhomberg Franziska , Moeslinger Thomas , Gottsauner-Wolf Michael 
Published: December 2014

Abstract

Aim:
Women do have longer QTc intervals compared to men. The aim of this study was to investigate as-yet undocumented effects of music on QTc intervals from electrocardiogram (ECG) recordings compared to various cardiovascular parameters of women in the prenatal phase.

Methods:
Forty-four healthy women in pregnancy were exposed to quiet surroundings (Q), self-selected slow music (S), and investigator-provided fast music (F) with different rhythm and frequency characteristics for 3 min each during their routine cardiotocography investigation. QTc intervals from ECG recordings were calculated according to the formulas of Bazett, Fridericia, and Sagie.

Results:
QTc durations (Bazett) increased from 0.396±0.02 (Q) to 0.401±0.02 s (S) and to 0.407±0.03 s (F). The increase in QTc (delta QTc) value for slow (S) and fast (F) music was found to be 5.3 (S) and 10.1 ms (F, milliseconds) when compared to quiescent (Q) conditions showing a highly significant tripled P-value of 0.002 (Q vs. F). Similar results were found using alternate frequency corrections (Fridericia, Sagie) – QTc (delta QTc): 8.1 (Fridericia) and 7.2 ms (Sagie), quiescent vs. F, with P<0.001. None of the tests for other cardiovascular parameters showed significant differences.

Conclusions:
As shown from our data, music with different rhythm and frequency characteristics may prolong QTc intervals in healthy preterm pregnant women and may add to other risk factors (e.g., preexisting QTc prolongation or application of QTc prolonging drugs). These data could prove to be relevant in the primary prevention recommendations for women at risk for arrhythmic patterns during pregnancy.
Influence of pressure on molecular packing and photochemical properties in three chalcone analogs
Authors: Bąkowicz Julia , Galica Tomasz , Turowska-Tyrk Ilona 
Published: December 2014

Abstract
The structures of 1-(4-methylphenyl)-3-(2-thienyl)prop-2-en-1-one, (I), 1-(5-bromo-2-hydroxyphenyl)-3-(2-furyl)prop-2-en-1-one, (II), and 1-(3-furyl)-3-[3-(trifluoromethyl)phenyl]prop-2-en-1-one, (III), were determined in ambient and high pressure conditions. The values of the intermolecular geometrical parameters describing possibilities of execution of [2+2] photodimerization were calculated and analysed. In the case of ambient pressure, for (I) they are close to the border valid for photoreactive compounds and for (II) and (III) they exclude the photochemical reaction in crystals. High pressure hardly changed the mutual orientation of adjacent molecules and largely the distance between them: in the case of (I), the distance was far below limits for compounds undergoing [2+2] photodimerization. The volume of free space was calculated for the studied compounds for different values of pressure and analysed in the context of possibilities of the [2+2] photodimerization.
Heart over mind: metabolic control of white adipose tissue and liver
Authors: Nakamura Michinari , Sadoshima Junichi 
Published: December 2014

Increasing evidence suggests that the heart controls the metabolism of peripheral organs. Olson and colleagues previously demonstrated that miR‐208a controls systemic energy homeostasis through the regulation of MED13 in cardiomyocytes (Grueter et al, ). In their follow‐up study in this issue of EMBO Molecular Medicine, white adipose tissue (WAT) and liver are identified as the physiological targets of cardiac MED13 signaling, most likely through cardiac‐derived circulating factors, which boost energy consumption by upregulating metabolic gene expression and increasing mitochondrial numbers (Baskin et al, ). In turn, increased energy expenditure in WAT and the liver confers leanness. These findings strengthen the evidence of metabolic crosstalk between the heart and peripheral tissues through cardiokines and also set the stage for the development of novel treatments for metabolic syndrome.
Vitamin D status and metabolic syndrome in the elderly: the Rotterdam Study
Authors: Vitezova A , Zillikens M C , van Herpt T T W , Sijbrands E J G , Hofman A , Uitterlinden A G , Franco O H , Kiefte-de Jong J C 
Published: December 2014

ObjectiveThe effects of vitamin D in the elderly are inconsistent. The aim of this study was to evaluate the association between vitamin D status and the metabolic syndrome (MetS) in the elderly, as well as between vitamin D status and the components of MetS (i.e. serum glucose, triglycerides (TG), HDL cholesterol (HDL-C), waist circumference (WC), and blood pressure (BP)).MethodsThe study was embedded in the Rotterdam Study, a population-based cohort of middle-aged and elderly adults. We analyzed data from 3240 people (median age 71.2 years) who did not have type 2 diabetes mellitus at baseline.ResultsWe found higher 25-hydroxyvitamin D (25(OH)D) concentrations associated with lower prevalence of MetS (odds ratio (OR); 95% CI: 0.61; 0.49, 0.77 for adequate levels (≥75 nmol/l) vs deficiency (<50 nmol/l). In addition, in analysis of the individual components, the ORs for adequate vs deficient vitamin D levels were: 0.66 (95% CI 0.53, 0.83) for elevated WC, 0.67 (95% CI 0.52, 0.86) for reduced HDL-C, 0.69 (95% CI 0.54, 0.88) for elevated TG, and 0.80 (95% CI 0.65, 0.99) for elevated fasting glucose. Vitamin D was not associated with elevated blood pressure, and ORs for adequacy vs deficiency were 0.82 (95% CI 0.65, 1.03).ConclusionHigher 25(OH)D concentrations in the elderly are associated with lower prevalence of MetS and, in particular, with more beneficial HDL-C, TG, WC, and serum glucose. Since the prevalence of vitamin D deficiency is common worldwide and its risk increases with age, if causality is proven, benefits of improving vitamin D status among the elderly may be great.