bluuebunny/crossref_metadata_2025_split huggingface dataset:
ACTN3 R577X Polymorphism and Explosive Leg-Muscle Power in Elite Basketball Players
Authors: Garatachea Nuria , Verde Zoraida , Santos-Lozano Alejandro , Yvert Thomas , Rodriguez-Romo Gabriel , Sarasa Francisco J. , Hernández-Sánchez Sonsoles , Santiago Catalina , Lucia Alejandro 
Published: February 2014

Purpose:To determine the association of the ACTN3 R577X polymorphism with leg-muscle explosive power in Spanish (white) elite basketball players and controls.Participants:100 (60 men) elite basketball players (cases) and 283 nonathletic controls.Methods:The authors assessed power performance by means of the vertical-squat and countermovement-jump tests.Results:Genotype distributions did not differ between groups (cases: 37.0% [RR], 42.0% [RX], and 21.0% [XX]; controls: 31.8% [RR], 49.8% [RX], and 18.4% [XX]; P = .353). The authors did not observe any effect of the ACTN3 R577X polymorphism on study phenotypes in either group, including when they performed the analyses separately in men and women. They found no association between the ACTN3 R577X polymorphism and the likelihood of being an elite basketball player using the dominant or the recessive model, and the results remained unaltered when the analyses were adjusted for sex, weight, height, and age or when performed for men and women separately.Conclusions:Although the ACTN3 R577X is associated with explosive muscle performance and this phenotype is important in the sport of basketball (ie, during jumps), the authors found no association with leg explosive power in elite basket players or with the status of being this type of athlete.
Does Polarized Training Improve Performance in Recreational Runners?
Authors: Muñoz Iker , Seiler Stephen , Bautista Javier , España Javier , Larumbe Eneko , Esteve-Lanao Jonathan 
Published: February 2014

Purpose:To quantify the impact of training-intensity distribution on 10K performance in recreational athletes.Methods:30 endurance runners were randomly assigned to a training program emphasizing low-intensity, sub-ventilatory-threshold (VT), polarized endurance-training distribution (PET) or a moderately high-intensity (between-thresholds) endurance-training program (BThET). Before the study, the subjects performed a maximal exercise test to determine VT and respiratory-compensation threshold (RCT), which allowed training to be controlled based on heart rate during each training session over the 10-wk intervention period. Subjects performed a 10-km race on the same course before and after the intervention period. Training was quantified based on the cumulative time spent in 3 intensity zones: zone 1 (low intensity, <VT), zone 2 (moderate intensity, between VT and RCT), and zone 3 (high intensity, >RCT). The contribution of total training time in each zone was controlled to have more low-intensity training in PET (±77/3/20), whereas for BThET the distribution was higher in zone 2 and lower in zone 1 (±46/35/19).Results:Both groups significantly improved their 10K time (39min18s ± 4min54s vs 37min19s ± 4min42s, P < .0001 for PET; 39min24s ± 3min54s vs 38min0s ± 4min24s, P < .001 for BThET). Improvements were 5.0% vs 3.6%, ~41 s difference at post-training-intervention. This difference was not significant. However, a subset analysis comparing the 12 runners who actually performed the most PET (n = 6) and BThET (n = 16) distributions showed greater improvement in PET by 1.29 standardized Cohen effect-size units (90% CI 0.31–2.27, P = .038).Conclusions:Polarized training can stimulate greater training effects than between-thresholds training in recreational runners.
Effect of Wearing Compression Stockings on Recovery After Mild Exercise-Induced Muscle Damage
Authors: Bieuzen François , Brisswalter Jeanick , Easthope Christopher , Vercruyssen Fabrice , Bernard Thierry , Hausswirth Christophe 
Published: February 2014

Background:Compression garments are increasingly popular in long-distance running events where they are used to limit cumulative fatigue and symptoms associated with mild exercise-induced muscle damage (EIMD). However, the effective benefits remain unclear.Objective:This study examined the effect of wearing compression stockings (CS) on EIMD indicators. Compression was applied during or after simulated trail races performed at competition pace in experienced off-road runners.Methods:Eleven highly trained male runners participated in 3 simulated trail races (15.6 km: uphill section 6.6 km, average gradient 13%, and downhill section 9.0 km, average gradient –9%) in a randomized crossover trial. The effect of wearing CS while running or during recovery was tested and compared with a control condition (ie, run and recovery without CS; non- CS). Indicators of muscle function, muscle damage (creatine kinase; CK), inflammation (interleukin-6; IL-6), and perceived muscle soreness were recorded at baseline (1 h before warm-up) and 1, 24, and 48 h after the run.Results:Perceived muscle soreness was likely to be lower when participants wore CS during trail running compared with the control condition (1 h postrun, 82% chance; 24 h postrun, 80% chance). A likely or possibly beneficial effect of wearing CS during running was also found for isometric peak torque at 1 h postrun (70% chance) and 24 h postrun (60% chance) and throughout the recovery period on countermovement jump, compared with non-CS. Possible, trivial, or unclear differences were observed for CK and IL-6 between all conditions.Conclusion:Wearing CS during simulated trail races mainly affects perceived leg soreness and muscle function. These benefits are visible very shortly after the start of the recovery period.
A Comparison of Laboratory-Based Kayak Testing Protocols
Authors: Jones Maddison J. , Peeling Peter 
Published: February 2014

Purpose:To compare the differences in peak oxygen uptake (VO2peak) and lactate threshold (LT2) between the 7 × 4-min incremental step test (7-ST) and the maximal accumulated oxygen deficit (MAOD) test protocols in sprint kayak athletes.Methods:Nine highly trained kayak athletes performed the 2 laboratory test protocols. The 7-ST involved six 4-min submaximal incremental stages, each separated by a 1-min recovery, before a 4-min all-out effort. The MAOD test involved four 4-min submaximal incremental stages (also with each stage separated by a 1-min recovery), followed by 20-min recovery and a 4-min all-out effort.Results:No statistically significant differences in VO2peak were recorded between the 2 protocols (P > .05). However, distance covered, power output, stroke rate, and speed were almost certainly greater in the MAOD test (magnitude-based inference: 99–100% positive), while blood lactate (BLa), heart rate (HR), and rating of perceived exertion (RPE) were likely lower (magnitude-based inference: 78–92% negative). The derived measures of LT2 (excluding HR) were not different between the 2 protocols.Conclusion:The results of this study suggest that both the 7-ST and MAOD test protocols are comparable with regard to the measurement of VO2peak and LT2 in highly trained sprint kayak athletes. However, since differences in the measures of distance traveled, power, stroke rate, HR, BLa, and RPE were reported in the maximal stage of the these 2 test protocols, their interchangeable use in a laboratory setting is not ideal if the data output is to be compared and contrasted over time.
Draft Genome Sequence of Pseudomonas moraviensis R28-S
Authors: Hunter Samuel S. , Yano Hirokazu , Loftie-Eaton Wesley , Hughes Julie , De Gelder Leen , Stragier Pieter , De Vos Paul , Settles Matthew L. , Top Eva M. 
Published: February 2014

ABSTRACT

We report the draft genome sequence of
Pseudomonas moraviensis
R28-S, isolated from the municipal wastewater treatment plant of Moscow, ID. The strain carries a native mercury resistance plasmid, poorly maintains introduced IncP-1 antibiotic resistance plasmids, and has been useful for studying the evolution of plasmid host range and stability.
The Genome Sequence of Bifidobacterium moukalabense DSM 27321 Highlights the Close Phylogenetic Relatedness with the Bifidobacterium dentium Taxon
Authors: Lugli Gabriele Andrea , Duranti Sabrina , Milani Christian , Turroni Francesca , Viappiani Alice , Mangifesta Marta , van Sinderen Douwe , Ventura Marco 
Published: February 2014

ABSTRACT

Bifidobacterium moukalabense
DSM 27321 is the reference strain for a recently described new bifidobacterial species that has been isolated from a wild west lowland gorilla. Here, we report the whole-genome sequence of DSM 27321, which supports very close phylogenetic relatedness with members of the
Bifidobacterium adolescentis
phylogenetic group and, in particular, the
Bifidobacterium dentium
taxon.
Draft Genome Sequence of the Boron-Tolerant and Moderately Halotolerant Bacterium Gracilibacillus boraciitolerans JCM 21714
T
Authors: Ahmed Iftikhar , Oshima Kenshiro , Suda Wataru , Kitamura Keiko , Iida Toshiya , Ohmori Yoshihiro , Fujiwara Toru , Hattori Masahira , Ohkuma Moriya 
Published: February 2014

ABSTRACT

Gracilibacillus boraciitolerans
JCM 21714
T
has been characterized as a highly boron-tolerant and moderately halotolerant bacterium. Here, we report the draft genome sequence of this strain. The genome sequence facilitates an understanding of the biochemical functions of boron and provides a base to identify the gene(s) involved in the boron tolerance mechanism of the strain.
Draft Genome Sequence of Extended-Spectrum β-Lactamase-Producing Klebsiella pneumoniae Isolated from a Patient in Lebanon
Authors: Tokajian Sima , Eisen Jonathan A. , Jospin Guillaume , Farra Anna , Coil David A. 
Published: February 2014

ABSTRACT

We present the draft genome sequence of extended-spectrum β-lactamase (ESBL)-producing
Klebsiella pneumoniae
isolated from a stool sample collected from a patient admitted for a gastrointestinal procedure. The draft genome sequence consists of 86 contigs, including a combined 5,632,663 bases with 57% G+C content.
Draft Genome Sequences of Extended-Spectrum β-Lactamase-Producing Escherichia coli Strains Isolated from Patients in Lebanon
Authors: Tokajian Sima , Eisen Jonathan A. , Jospin Guillaume , Farra Anna , Coil David A. 
Published: February 2014

ABSTRACT

We present the draft genome sequences of nine extended-spectrum β-lactamase (ESBL)-producing
Escherichia coli
strains isolated from stool samples collected from patients admitted for gastrointestinal and urological procedures/surgeries. An average of 3,889,300 paired-end reads per sample were generated, which assembled in 77 to 157 contigs.
Draft Genome Sequence of Lactobacillus rhamnosus 2166
Authors: Karlyshev Andrey V. , Melnikov Vyacheslav G. , Kosarev Igor V. , Abramov Vyacheslav M. 
Published: February 2014

ABSTRACT

In this report, we present a draft sequence of the genome of
Lactobacillus rhamnosus
strain 2166, a potential novel probiotic. Genome annotation and read mapping onto a reference genome of
L. rhamnosus
strain GG allowed for the identification of the differences and similarities in the genomic contents and gene arrangements of these strains.
Laggan Field 3D geological modelling: case study
Authors: Kheidri L. H. , Cailly F. , Jones K. , De Senneville A. , Gray R. 
Published: February 2014

Abstract
Drilling of Laggan development wells commenced in summer 2013 and finalization of well locations required updated reservoir models, fully incorporating the 2009 baseline seismic. Consequently, geomodelling workflows have been developed based on the 3D seismic, petrophysical and production test data. The reservoir model building methodology for Laggan consists of the following steps: (1) construction of a classical structural framework using seismic interpreted fault sticks, top reservoir depth surface and the overall reservoir thickness map estimated from seismic data; (2) building of zones model integrating well data, seismic and sedimentological concepts; and (3) generation of a 3D facies and petrophysical model constrained by seismic and well data, including well test results to calibrate the permeability.
A reasonable match of the well test data was achieved without any major adjustments to the reservoir model. This methodology has led to the construction of a robust reservoir description, capturing the geological knowledge of the turbidite system and constrained by well and seismic data.
The new Laggan model is now considered ‘fit for purpose’ for well locations update, well type and well number confirmation and development of the Reservoir Management Plan.
Combined Anti-CD40 and Anti–IL-23 Monoclonal Antibody Therapy Effectively Suppresses Tumor Growth and Metastases
Authors: von Scheidt Bianca , Leung Patrick S.K. , Yong Michelle C.R. , Zhang Yu , Towne Jennifer E. , Smyth Mark J. , Teng Michele W.L. 
Published: February 2014

AbstractTumor-induced immunosuppression remains one of the major obstacles to many potentially effective cancer therapies and vaccines. Host interleukin (IL)-23 suppresses the immune response during tumor initiation, growth, and metastases, and neutralization of IL-23 causes IL-12–dependent antitumor effects. Here, we report that combining agonistic anti-CD40 monoclonal antibodies (mAb) to drive IL-12 production and anti–IL-23 mAbs to counter the tumor promoting effects of IL-23 has greater antitumor activity than either agent alone. This increased antitumor efficacy was observed in several experimental and spontaneous lung metastases models as well as in models of de novo carcinogenesis. The combination effects were dependent on host IL-12, perforin, IFN-γ, natural killer, and/or T cells and independent of host B cells and IFN-αβ sensitivity. Interestingly, in the experimental lung metastases tumor models, we observed that intracellular IL-23 production was specifically restricted to MHC-IIhiCD11c+CD11b+ cells. Furthermore, an increase in proportion of these IL-23–producing cells was detected only in tumor models where IL-23 neutralization was therapeutic. Overall, these data suggest the clinical potential of using anti-CD40 (push) and anti–IL-23 mAbs (pull) to tip the IL-12/23 balance in established tumors. Cancer Res; 74(9); 2412–21. ©2014 AACR.
A Systems Biology Approach Identifies Effective Tumor–Stroma Common Targets for Oral Squamous Cell Carcinoma
Authors: Meng Wenxia , Wu Yun , He Xin , Liu Chuanxia , Gao Qinghong , Ge Lin , Wu Lanyan , Liu Ying , Guo Yiqing , Li Xiaoyu , Liu Yurong , Chen Sixiu , Kong Xiangli , Liang Zhi , Zhou Hongmei 
Published: February 2014

Abstract
The complex interactions between cancer cells and their surrounding stromal microenvironment play important roles in tumor initiation and progression and represent viable targets for therapeutic intervention. Here, we propose a concept of common target perturbation (CTP). CTP acts simultaneously on the same target in both the tumor and its stroma that generates a bilateral disruption for potentially improved cancer therapy. To employ this concept, we designed a systems biology strategy by combining experiment and computation to identify potential common target. Through progressive cycles of identification, TGF-β receptor III (TβRIII) is found as an epithelial–mesenchymal common target in oral squamous cell carcinoma. Simultaneous perturbation of TβRIII in the oral cancerous epithelial cells and their adjacent carcinoma-associated fibroblasts effectively inhibits tumor growth in vivo, and shows superiority to the unilateral perturbation of TβRIII in either cell type alone. This study indicates the strong potential to identify therapeutic targets by considering cancer cells and their adjacent stroma simultaneously. The CTP concept combined with our common target discovery strategy provides a framework for future targeted cancer combinatorial therapies. Cancer Res; 74(8); 2306–15. ©2014 AACR.
Androgen Receptor Splice Variants Determine Taxane Sensitivity in Prostate Cancer
Authors: Thadani-Mulero Maria , Portella Luigi , Sun Shihua , Sung Matthew , Matov Alexandre , Vessella Robert L. , Corey Eva , Nanus David M. , Plymate Stephen R. , Giannakakou Paraskevi 
Published: February 2014

Abstract
Prostate cancer growth depends on androgen receptor signaling. Androgen ablation therapy induces expression of constitutively active androgen receptor splice variants that drive disease progression. Taxanes are a standard of care therapy in castration-resistant prostate cancer (CRPC); however, mechanisms underlying the clinical activity of taxanes are poorly understood. Recent work suggests that the microtubule network of prostate cells is critical for androgen receptor nuclear translocation and activity. In this study, we used a set of androgen receptor deletion mutants to identify the microtubule-binding domain of the androgen receptor, which encompasses the DNA binding domain plus hinge region. We report that two clinically relevant androgen receptor splice variants, ARv567 and ARv7, differentially associate with microtubules and dynein motor protein, thereby resulting in differential taxane sensitivity in vitro and in vivo. ARv7, which lacks the hinge region, did not co-sediment with microtubules or coprecipitate with dynein motor protein, unlike ARv567. Mechanistic investigations revealed that the nuclear accumulation and transcriptional activity of ARv7 was unaffected by taxane treatment. In contrast, the microtubule-interacting splice variant ARv567 was sensitive to taxane-induced microtubule stabilization. In ARv567-expressing LuCap86.2 tumor xenografts, docetaxel treatment was highly efficacious, whereas ARv7-expressing LuCap23.1 tumor xenografts displayed docetaxel resistance. Our results suggest that androgen receptor variants that accumulate in CRPC cells utilize distinct pathways of nuclear import that affect the antitumor efficacy of taxanes, suggesting a mechanistic rationale to customize treatments for patients with CRPC, which might improve outcomes. Cancer Res; 74(8); 2270–82. ©2014 AACR.
RNA Editome Imbalance in Hepatocellular Carcinoma
Authors: Qi Lihua , Chan Tim Hon Man , Tenen Daniel G. , Chen Leilei 
Published: February 2014

Abstract
Adenosine-to-inosine conversion (A-to-I editing), a posttranscriptional modification on RNA, contributes to extensive transcriptome diversity. A-to-I editing is a hydrolytic deamination process, catalyzed by adenosine deAminase acting on double-stranded RNA (ADAR) family of enzymes. ADARs are essential for normal mammalian development, and disturbance in RNA editing has been implicated in various pathologic disorders, including cancer. Thanks to next-generation sequencing, rich databases of transcriptome evolution for cancer development at the resolution of single nucleotide have been generated. Extensive bioinformatic analysis revealed a complex picture of RNA editing change during transformation. Cancer displayed global hypoediting of Alu-repetitive elements with gene-specific editing pattern. In particular, hepatocellular carcinoma editome is severely disrupted and characterized by hyper- and hypoediting of different genes, such as hyperedited AZIN1 (antizyme inhibitor 1) and FLNB (filamin B, β) and hypoedited COPA (coatomer protein complex, subunit α). In hepatocellular carcinoma, not only the recoding editing in exons, but also the editing in noncoding regions (e.g., Alu-repetitive elements and microRNA) displays such complex editing pattern with site-specific editing trend. In this review, we will discuss current research progress on the involvement of abnormal A-to-I editing in cancer development, more specifically on hepatocellular carcinoma. Cancer Res; 74(5); 1301–6. ©2014 AACR.
Deficiency of the MicroRNA-31–MicroRNA-720 Pathway in the Plasma and Endothelial Progenitor Cells From Patients With Coronary Artery Disease
Authors: Wang Hsei-Wei , Huang Tse-Shun , Lo Hung-Hao , Huang Po-Hsun , Lin Chih-Ching , Chang Shing-Jyh , Liao Ko-Hsun , Tsai Chin-Han , Chan Chia-Hao , Tsai Cheng-Fong , Cheng Yi-Chieh , Chiu Ya-Ling , Tsai Tsung-Neng , Cheng Cheng-Chung , Cheng Shu-Meng 
Published: February 2014

Objective—
Defects in angiogenesis/vasculogenesis or vessel repair are major complications of coronary artery disease (CAD). Endothelial progenitor cells (EPCs) play a fundamental role in postnatal vascular repair and CAD. The role of microRNAs in CAD pathogenesis and their potential as biomarkers remain to be elucidated.

Approach and Results—

MicroRNA-31 (miR-31) level in both the plasma and EPCs of patients with CAD is found lower. miR-31 regulates EPC activities by targeting FAT atypical cadherin 4 and thromboxane A2 receptor, which show increased expression in CAD EPCs. Overexpressing miR-31 in CAD EPCs rescued their angiogenic and vasculogenic abilities both in vitro and in vivo. When exploring approaches to restore endogenous miR-31, we found that far-infrared treatment enhanced the expression of not only miR-31, but also miR-720 in CAD EPCs. miR-720, which was also decreased in EPCs and the plasma of patients with CAD, stimulated EPC activity by targeting vasohibin 1. The miR720–vasohibin 1 pair was shown to be downstream of FAT atypical cadherin 4, but not of thromboxane A2 receptor. FAT atypical cadherin 4 inhibited miR-720 expression via repression of the planar cell polarity signaling gene four-jointed box 1 (
FJX1
), which was required for miR-720 expression through a hypoxia-inducible factor 1, α subunit–dependent mechanism. Restoring miR-720 level strengthened activity of CAD EPCs. The miR-31–miR-720 pathway is shown critical to EPC activation and that downregulation of this pathway contributes to CAD pathogenesis. Circulating levels of miR-31, miR-720, and vasohibin 1 have the potential to allow early diagnosis of CAD and to act as prognosis biomarkers for CAD and other EPC-related diseases.

Conclusions—
Manipulating the expression of the miR-31–miR-720 pathway in malfunction EPCs should help develop novel therapeutic modalities.
Contribution of Kv7.4/Kv7.5 Heteromers to Intrinsic and Calcitonin Gene-Related Peptide–Induced Cerebral Reactivity
Authors: Chadha Preet S. , Jepps Thomas A. , Carr Georgina , Stott Jennifer B. , Zhu Hei-Lei , Cole William C. , Greenwood Iain A. 
Published: February 2014

Objective—
Middle cerebral artery (MCA) diameter is regulated by inherent myogenic activity and the effect of potent vasodilators such as calcitonin gene-related peptide (CGRP). Previous studies showed that MCAs express KCNQ1, 4, and 5 potassium channel genes, and the expression products (Kv7 channels) participate in the myogenic control of MCA diameter. The present study investigated the contribution of Kv7.4 and Kv7.5 isoforms to myogenic and CGRP regulation of MCA diameter and determined whether they were affected in hypertensive animals.

Approach and Results—

Isometric tension recordings performed on MCA from normotensive rats produced CGRP vasodilations that were inhibited by the pan-Kv7 channel blocker linopirdine (
P
<0.01) and after transfection of arteries with siRNA against KCNQ4 (
P
<0.01) but not KCNQ5. However, isobaric myography revealed that myogenic constriction in response to increases in intravascular pressure (20–80 mm Hg) was affected by both KCNQ4 and KCNQ5 siRNA. Proximity ligation assay signals were equally abundant for Kv7.4/Kv7.4 or Kv7.4/Kv7.5 antibody combinations but minimal for Kv7.5/Kv7.5 antibodies or Kv7.4/7.1 combinations. In contrast to systemic arteries, Kv7 function and Kv7.4 abundance in MCA were not altered in hypertensive rats.

Conclusions—
This study reveals, for the first time to our knowledge, that in cerebral arteries, Kv7.4 and Kv7.5 proteins exist predominantly as a functional heterotetramer, which regulates intrinsic myogenicity and vasodilation attributed to CGRP. Surprisingly, unlike systemic arteries, Kv7 activity in MCAs is not affected by the development of hypertension, and CGRP-mediated vasodilation is well maintained. As such, cerebrovascular Kv7 channels could be amenable for therapeutic targeting in conditions such as cerebral vasospasm.