bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimal methods of vitamin D supplementation to prevent acute respiratory infections: a systematic review, dose–response and pairwise meta-analysis of randomized controlled trials
Authors: Wang Chih-Hung , Porta Lorenzo , Yang Ting-Kai , Wang Yu-Hsiang , Wu Tsung-Hung , Qian Frank , Han Yin-Yi , Sheng Wang-Huei , Chen Shyr-Chyr , Lee Chien-Chang , Chang Shan-Chwen 
Published: August 2024

Abstract
Background
Vitamin D supplementation may prevent acute respiratory infections (ARIs). This study aimed to identify the optimal methods of vitamin D supplementation.

Methods
PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, and the ClinicalTrials.gov registry were searched from database inception through July 13, 2023. Randomized-controlled trials (RCTs) were included. Data were pooled using random-effects model. The primary outcome was the proportion of participants with one or more ARIs.

Results
The analysis included 43 RCTs with 49320 participants. Forty RCTs were considered to be at low risk for bias. The main pairwise meta-analysis indicated there were no significant preventive effects of vitamin D supplementation against ARIs (risk ratio [RR]: 0.99, 95% confidence interval [CI]: 0.97 to 1.01, I2 = 49.6%). The subgroup dose–response meta-analysis indicated that the optimal vitamin D supplementation doses ranged between 400–1200 IU/day for both summer-sparing and winter-dominant subgroups. The subgroup pairwise meta-analysis also revealed significant preventive effects of vitamin D supplementation in subgroups of daily dosing (RR: 0.92, 95% CI: 0.85 to 0.99, I2 = 55.7%, number needed to treat [NNT]: 36), trials duration < 4 months (RR: 0.81, 95% CI: 0.67 to 0.97, I2 = 48.8%, NNT: 16), summer-sparing seasons (RR: 0.85, 95% CI: 0.74 to 0.98, I2 = 55.8%, NNT: 26), and winter-dominant seasons (RR: 0.79, 95% CI: 0.71 to 0.89, I2 = 9.7%, NNT: 10).

Conclusion
Vitamin D supplementation may slightly prevent ARIs when taken daily at doses between 400 and 1200 IU/d during spring, autumn, or winter, which should be further examined in future clinical trials.
Mass media exposure and sociodemographic factors associated with malaria vaccine awareness among women of childbearing age in Ghana
Authors: Mensah Emmanuel Angmorteh , Duah Henry Ofori , Olomofe Charles , Quinn Megan 
Published: August 2024

Abstract
Background
The development, approval and adoption of the malaria vaccine has provided effective supplemental protection against malaria for children in Ghana. However, heightened awareness of the new vaccine will play a critical role in its mass deployment and acceptance among potential recipients. This study therefore determined the sociodemographic characteristics associated with malaria vaccine awareness and ascertained the influence of traditional media exposure on awareness in Ghana.

Methods
The study used the Demographic and Health Survey 2019 Malaria Indicator Survey Data. After necessary recoding, chi-square and complex survey bivariate/multivariate logistic regression analyses were performed using STATA 18.0.

Results
Among the participants, 35.65% (95% CI: 33.83%–37.51%) had heard about the malaria vaccine. Positive predictors of awareness included higher education attainment (AOR = 1.92, 95% CI: 1.28–2.88), rural residency (AOR = 1.28, 95% CI: 1.05–1.56) and being in the northern part of the country (AOR 1.54, 95% CI: 1.14–2.07). Other positive predictors of awareness were Guan ethnicity (AOR = 1.75, 95% CI: 1.09–2.81), malaria health education (AOR = 1.73, 95% CI: 1.45–2.07) and radio set ownership (AOR = 1.39, 95% CI: 1.19–1.62). Television ownership (AOR = 0.84, 95% CI: 0.69–1.01) showed no significant relationship with awareness.

Conclusion
The general nationwide awareness has not yet reached a desirable level. This study suggests that, the awareness drive, preferably through radio campaigns, must target individuals with less than tertiary education and urban communities. Awareness campaigns on televisions should be evaluated and possibly redesigned for effectiveness.
Application of the wildland fire emissions inventory system to estimate fire emissions on forest lands of the United States
Authors: Smith James E. , Billmire Michael , French Nancy H.F. , Domke Grant M. 
Published: August 2024

Abstract
Background
Forests are significant terrestrial biomes for carbon storage, and annual carbon accumulation of forest biomass contributes offsets affecting net greenhouse gases in the atmosphere. The immediate loss of stored carbon through fire on forest lands reduces the annual offsets provided by forests. As such, the United States reporting includes annual estimates of direct fire emissions in conjunction with the overall forest stock and change estimates as a part of national greenhouse gas inventories within the United Nations Framework Convention on Climate Change. Forest fire emissions reported for the United States, such as the 129 Tg CO2 reported for 2022, are based on the Wildland Fire Emissions Inventory System (WFEIS). Current WFEIS estimates are included in the Inventory of U.S. Greenhouse Gas Emissions and Sinks: 1990–2022 published in 2024 by the United States Environmental Protection Agency. Here, we describe WFEIS the fire emissions inventory system we used to address current information needs, and an analysis to confirm compatibility of carbon mass between estimated forest fire emissions and carbon in forest stocks.

Results
The summaries of emissions from forests are consistent with previous reports that show rates and interannual variability in emissions and forest land area burned are generally greater in recent years relative to the 1990s. Both emissions and interannual variability are greater in the western United States. The years with the highest CO2 emissions from forest fires on the 48 conterminous states plus Alaska were 2004, 2005, and 2015. In some years, Alaska emissions exceed those of the 48 conterminous states, such as in 2022, for example. Comparison of forest fire emission to forest carbon stocks indicate there is unlikely any serious disconnect between inventory and fire emissions estimates.

Conclusions
The WFEIS system is a user-driven approach made available via a web browser. Model results are compatible with the scope and reporting needs of the annual national greenhouse gas inventories.
Safety and feasibility of intravenous administration of a single dose of allogenic-Muse cells to treat human cervical traumatic spinal cord injury: a clinical trial
Authors: Koda Masao , Imagama Shiro , Nakashima Hiroaki , Ito Sadayuki , Segi Naoki , Ouchida Jun , Suda Kota , Harmon Matsumoto Satoko , Komatsu Miki , Endo Toshiki , Suzuki Shinsuke , Inami Satoshi , Ueda Haruki , Miyagi Masayuki , Inoue Gen , Takaso Masashi , Nagata Keiji , Yamada Hiroshi , Kamei Naosuke , Nakamae Toshio , Suzuki Hidenori , Nishida Norihiro , Funaba Masahiro , Kumagai Gentaro , Furuya Takeo , Yamato Yu , Funayama Toru , Takahashi Hiroshi , Yamazaki Masashi 
Published: August 2024

Abstract
Introduction
Spinal cord injury (SCI) is a devastating injury and remains one of the largest medical and social burdens because of its intractable nature. According to the recent advances in stem cell biology, the possibility of spinal cord regeneration and functional restoration has been suggested by introducing appropriate stem cells. Multilineage-differentiating stress enduring (Muse) cells are a type of nontumorigenic endogenous reparative stem cell. The positive results of Muse cell transplantation for SCI was shown previously. As a first step for clinical application in human SCI, we conducted a clinical trial aiming to confirm the safety and feasibility of intravenously injected donor-Muse cells.

Methods
The study design of the current trial was a prospective, multicenter, nonrandomized, nonblinded, single-arm study. The clinical trial registration number was JRCT1080224764. Patients with a cervical SCI with a neurological level of injury C4 to C7 with the severity of modified Frankel classification B1 and B2 were included. A primary endpoint was set for safety and feasibility. Our protocol was approved by the PMDA, and the trial was funded by the Life Science Institute, Tokyo, Japan. The present clinical trial recruited 10 participants (8 males and 2 females) with an average age of 49.3 ± 21.2 years old. All 10 participants received a single dose of allogenic CL2020 (a total of 15 × 106 cells, 2.1–2.7 × 105 cells/kg of body weight), which is a Muse cell-based product produced from human mesenchymal stem cells, by an intravenous drip.

Results
There were two reported severe adverse events, both of which were determined to have no causal relationship with Muse cell treatment. The change in the ISNCSCI motor score, the activity of daily living and quality of life scores showed statistically significant improvements compared to those data at the time of CL2020 administration.

Conclusion
In the present trial, no safety concerns were identified, and Muse cell product transplantation demonstrated good tolerability. Future clinical trials with appropriate study designs incorporating a control arm will clarify the definitive efficacy of single-dose allogenic Muse cell treatment with intravenous administration to treat SCI.
Trial registration: jRCT, JRCT1080224764. Registered 03 July 2019, https://jrct.niph.go.jp/latest-detail/jRCT1080224764.
Human brain organoids containing microglia that have arisen innately adapt to a β-amyloid challenge better than those in which microglia are integrated by co-culture
Authors: Wenzel Tyler J. , Desjarlais Joseph D. , Mousseau Darrell D. 
Published: August 2024

Abstract
Background
Alzheimer disease (AD) is a heterogenous and multifactorial disease, and its pathology is partly driven by microglia and their activated phenotype. Brain organoids (BOs) are gaining prominence as a relevant model of the human brain for the study of AD; however, BOs are commonly devoid of microglia. To overcome this limitation, current protocols incorporate microglia through either (1) co-culture (BO co-culture), or (2) molecular manipulation at critical windows of BO development to have microglia arise innately (BO innate cultures). It is currently unclear whether the microglia incorporated into BOs by either of these two protocols differ in function.

Methods
At in vitro day 90, BO innate cultures and BO-co-cultures were challenged with the AD-related β-amyloid peptide (Aβ) for up to 72 h. After Aβ challenge, BOs were collected for immunoblotting. Immunoblots compared immunodensity and protein banding of Aβ and ionized calcium-binding adapter molecule 1 (IBA1, a marker of microglial activation) in BOs. The translational potential of these observations was supported using 56 human cortical samples from neurocognitively normal donors and patients with early-onset AD and late-onset AD. Statistical analyses were conducted using the Kruskal–Wallis test, a two-way ANOVA, or a simple linear regression, and where applicable, followed by Dunn’s or Sidak’s test.

Results
We show that BO co-cultures promote Aβ oligomerization as early as 24 h and this coincides with a significant increase in IBA1 levels. In contrast, the Aβs do not oligomerize in BO innate cultures and the IBA1 response was modest and only emerged after 48 h. In human cortical samples, we found IBA1 levels correlated with age at onset, age at death, and the putative diagnostic Aβ(1–42)/Aβ(1–40) ratio (particularly in their oligomeric forms) in a sex-dependent manner.

Conclusions
Our unique observations suggest that BOs with innate microglia model the response of a healthy brain to Aβ, and by extension the initial stages of Aβ challenge. It would be impossible to model these early stages of pathogenesis in BOs where microglia are already compromised, such as those with microglia incorporated by co-culture.
Do leaf lignin content or leaf mass-to-area bias the estimation of intrinsic water use efficiency from leaf bulk δ13C? A test with seedlings from five oak species
Authors: Ghouil Hana , Richard Béatrice , Montpied Pierre , Dreyer Erwin , Brendel Oliver 
Published: August 2024

Abstract
Key Message
Leaves of seedlings from five oak species (Quercus robur L.; Q. pubescens L.; Q. suber L.; Q. afares Pomel; Q. ilex L.) displayed large, mainly inter-specific, differences in leaf mass-to-area ratio (LMA) and lignin content, as well as in the 13C composition of bulk leaf biomass. The variation in leaf lignin content and LMA did not impact the offset between the 13C composition measured in bulk leaf material versus soluble sugars. This observation, as well as the similar correlations between intrinsic leaf water use efficiency and the 13C compositions of bulk material or soluble sugars extracted from leaves, confirms their reliable use as a proxy for the former even when there is a large variation in LMA or lignin among samples.

Context
Carbon isotope composition (δ13C) of bulk leaf biomass is frequently used as a proxy for intrinsic water use efficiency (iWUE) in large-scale intra- and inter-specific comparisons. However, post-photosynthetic 13C discrimination during the synthesis of lignin combined with differences in leaf mass-to-area ratio (LMA) may bias the relationship between δ13C of bulk leaf matter and iWUE and thus its use as a proxy of iWUE.

Aims
To quantify the impact of differences in lignin content and LMA on the relationship between δ13C of bulk leaf biomass and iWUE over a large gradient of lignin contents across five oak species (deciduous: Quercus robur, Q. pubescens, Q. afares and evergreen: Q. ilex and Q. suber).

Methods
We measured lignin content, LMA, and δ13C of bulk leaf biomass and of soluble sugars extracted from the leaves, as well as intrinsic water use efficiency (derived from leaf gas exchange) in seedlings of the five oak species grown under common conditions in a greenhouse and measured in a climate chamber.

Results
There was a large range (mainly across species) in lignin content (4 to 33%) and LMA (60–180 g m−2). δ13C of bulk leaf biomass and soluble sugars were tightly correlated, showing a significant mean offset of − 0.4‰. This offset was stable across species and not correlated to the lignin content of the leaves. A very loose correlation was found between the offset and LMA, mainly due to one species.

Conclusion
Our results are a demonstration that potential variations in leaf lignin content or LMA have no or only a little effect on the δ13C of bulk leaf biomass. They are unlikely to cause a bias when using bulk leaf δ13C as a proxy for variations in intrinsic water use efficiency among Mediterranean and temperate broad-leaf forest tree species.
Upper and lower solutions for an integral boundary problem with two different orders $\left ( p,q\right ) $-fractional difference
Authors: Mesmouli Mouataz Billah , Al-Askar Farah M. , Mohammed Wael W. 
Published: August 2024

AbstractIn this paper, a $\left ( p,q\right ) $

(
p
,
q
)

-fractional nonlinear difference equation of different orders is considered and discussed. With the help of $\left ( p,q\right ) $

(
p
,
q
)

-calculus for integrals and derivatives properties, we convert the main integral boundary value problem (IBVP) to an equivalent solution in the form of an integral equation, we use the upper–lower solution technique to prove the existence of positive solutions. We present an example of the IBVP to apply and demonstrate the results of our method.
Attitudes towards depression and its treatment among white, hispanic, and multiracial adults
Authors: Feliciano Leilani , Erdal Kristi , Sandal Gro Mjeldheim 
Published: August 2024

Abstract
Background
Depression is present in all societies and affects members of all racial and ethnic groups. However, attitudes about depression differ across groups and have been shown to impact help-seeking behaviors, preferences for treatments, and compliance with treatments.

Methods
Taking a cross-cultural approach, this project used a case vignette of depression to examine race/ethnic group differences in attitudes about depression and its treatment among young adults in the U.S.

Results
Data analyses revealed significant racial/ethnic group differences in attitudes as well as the treatments/strategies participants reported they would use. Gender x race/ethnicity interactions revealed that White and Multiracial/ethnic men were more likely to believe the vignette character should find a partner to help with symptoms, while White and Multiracial/ethnic women did not endorse those strategies. Hispanic men and women did not show a gender difference in that strategy, but gender differences were observed in other strategies. In a rare comparison, majority-minority Multiracial/ethnic participants (i.e., White selected as one of their races/ethnicities) rated identified helpers and treatments similarly to White participants and significantly higher than multiple-minority Multiracial participants (i.e., White not selected as one of their races/ethnicities).

Conclusions
Findings supported previous research that indicates different U.S. racial/ethnic group ideas of depression and its treatment are potentially linked with cultural values, and we suggest that investigating these more fine-grained group differences can help to inform treating professionals as well as public health messages.
German translation and cross-cultural adaptation of the Vestibular Schwannoma Quality of Life Index (VSQOL)
Authors: Rutenkröger Mareike , Wandke Svenja , Gempt Jens , Dührsen Lasse , Scheer Maximilian , Strauss Christian , Führes Hannah 
Published: August 2024

AbstractBackgroundVestibular schwannomas (VSs) are benign tumors of the vestibulocochlear nerve that often cause significant neurological and functional impairment. Patient-reported outcomes, including quality of life (QoL), are essential for understanding the overall impact of VS and its treatment. This study aimed to translate and culturally adapt the Vestibular Schwannoma Quality of Life (VSQOL) Index into German to expand its relevance to German-speaking populations.MethodsWe used a qualitative approach including translation and cognitive interviews with 10 patients who underwent VS surgery. The translation process followed the TRAPD protocol to ensure linguistic and conceptual accuracy. Cognitive interviews assessed the comprehensibility and relevance of the translated questionnaire.ResultsThe translation showed remarkable consistency between translators, with minor discrepancies resolved by consensus. Cognitive interviews provided valuable insights that led to refinements in item wording. Participants emphasized the importance of an additional item on physician referrals, reflecting differences in health care systems between the United States and Germany.ConclusionsThe German VSQOL provides a comprehensive tool for assessing QoL in patients with VS that integrates patient-centered dimensions. A Validation study is underway to establish its reliability and validity.
Video watching and hands-on experiments to learn science: what can each uniquely contribute?
Authors: Shao Faxian , Tang Li , Zhang Huan 
Published: August 2024

AbstractAn experimental group and control group pretest and posttest design was used to conduct qualitative coding and quantitative analysis on two classes in grade 5. The aim was to investigate whether science education videos can be used in place of simulation experiments. The results showed that (1) in terms of scientific knowledge, the difference between science videos and hands-on experiments was not significant; (2) in terms of modeling capability, the hands-on class had better results regarding the perception and application of materials; and (3) more precise experiments had better effects on health behavior, but there was no significant difference between imprecise experiments and science videos. In the absence of laboratory equipment, science videos could therefore be an alternative solution.
Giant right and left atrium: spectrum of rheumatic triple valve disease (double hit with double impact): a case report and review of the literature
Authors: Karuru Uma Devi , Tummala Sadanand Reddy , Gautam Paladugu Srinivas , Naveen T. , Kanjerla Kiran Kumar , Mysore Sai Kumar 
Published: August 2024

Abstract
Background
Rheumatic heart disease (RHD) continues to pose a significant burden on global health, particularly in socioeconomically disadvantaged populations. We present the case of a 38-year-old woman with severe multivalvular RHD and giant atria, highlighting the challenges and complexities of managing this condition.

Case presentation
The patient presented with progressively worsening dyspnoea, signs of right heart failure, and severe valvular abnormalities. Diagnostic evaluations revealed severe mixed mitral valve disease in the form of mitral stenosis and regurgitation, along with involvement of the aortic and tricuspid valves, leading to significant enlargement of both atria. Despite facing socioeconomic constraints and poor adherence to treatment, the patient underwent successful surgical intervention, resulting in remarkable symptomatic improvement.

Conclusions
Through this case, we emphasise the importance of early detection, comprehensive management strategies, and multidisciplinary care in addressing the complexities of RHD. Despite the challenges posed by socioeconomic disparities, positive outcomes can be achieved with timely diagnosis and appropriate intervention. This case underscores the need for targeted efforts to improve access to healthcare resources and reduce the global burden of RHD.
Swollen speech: a case study of tongue abscess detected via imaging technology
Authors: Rudrakshi Anusha , Ramdurg Praveenkumar , Naveen S. 
Published: August 2024

Abstract
Background
A tongue abscess is a rare but serious condition marked by a localized accumulation of pus within the tongue’s tissues. Despite the tongue’s frequent exposure to trauma, bites, and foreign objects, it typically resists infections. However, an acute lingual abscess can be life-threatening due to the risk of airway obstruction from tongue swelling. Common symptoms include increasing pain, fever, swelling, immobility, edema, and redness of the tongue. The case report aimed to highlight the unusual clinical presentation and location of a deep-seated abscess in the anterior third of the tongue, as well as the radiographic approach to diagnosing the lesion.

Case presentation
A 30-year-old female patient presented with sudden onset tongue pain and swelling persisting for 4–5 days. Clinical examination revealed a solitary diffuse swelling in the anterior two-thirds of the tongue, approximately 3 × 2 cm in size. Diagnostic imaging, including a plain radiograph, ultrasound (USG), and MRI, was conducted. The patient was hospitalized for incision and drainage of the abscess, followed by a 5-day course of intravenous antibiotics.

Conclusions
A tongue abscess, though rare, is a serious condition indicated by tongue swelling, pain, fever, dysphagia, and dyspnea. Diagnosis involves physical examinations and imaging studies like ultrasound or MRI. Immediate treatment includes maintaining airway patency, draining the abscess, and administering broad-spectrum antibiotics. Prompt recognition and treatment are essential to prevent severe complications such as airway obstruction and sepsis. Regular follow-up ensures complete resolution and prevents recurrence.