bluuebunny/crossref_metadata_2025_split huggingface dataset:
3D Printed Nanosensors for Cancer Diagnosis: Advances and Future Perspective
Authors: Gupta Babita , Malviya Rishabha , Srivastava Saurabh , Ahmad Irfan , Rab Safia Obaidur , Singh Deependra Pratap 
Published: August 2024

:
Cancer is the leading cause of mortality worldwide, requiring continuous advancements in diagnosis
and treatment. Traditional methods often lack sensitivity and specificity, leading to the need for new methods.
3D printing has emerged as a transformative tool in cancer diagnosis, offering the potential for precise
and customizable nanosensors. These advancements are critical in cancer research, aiming to improve early detection
and monitoring of tumors. In current times, the usage of the 3D printing technique has been more prevalent
as a flexible medium for the production of accurate and adaptable nanosensors characterized by exceptional
sensitivity and specificity. The study aims to enhance early cancer diagnosis and prognosis by developing
advanced 3D-printed nanosensors using 3D printing technology. The research explores various 3D printing
techniques, design strategies, and functionalization strategies for cancer-specific biomarkers. The integration
of these nanosensors with detection modalities like fluorescence, electrochemical, and surface-enhanced Raman
spectroscopy is also evaluated. The study explores the use of inkjet printing, stereolithography, and fused
deposition modeling to create nanostructures with enhanced performance. It also discusses the design and functionalization
methods for targeting cancer indicators. The integration of 3D-printed nanosensors with multiple
detection modalities, including fluorescence, electrochemical, and surface-enhanced Raman spectroscopy, enables
rapid and reliable cancer diagnosis. The results show improved sensitivity and specificity for cancer biomarkers,
enabling early detection of tumor indicators and circulating cells. The study highlights the potential
of 3D-printed nanosensors to transform cancer diagnosis by enabling highly sensitive and specific detection of
tumor biomarkers. It signifies a pivotal step forward in cancer diagnostics, showcasing the capacity of 3D
printing technology to produce advanced nanosensors that can significantly improve early cancer detection
and patient outcomes.
Sonochemical Isomerization of [5,6]-open Adducts of C60 Fullerene with Camphor Derivatives into [6,6]-closed Ones
Authors: Kinzyabaeva Zemfira S. , Akhmetov Arslan R. 
Published: August 2024

:
For the first time, a sonochemical method has been developed for the structural
rearrangement of [5,6]-open mono- and poly adducts of fullerene C60 containing spirocyclic
fragments of camphor analogs into [6,6]-closed isomers. The isomerization reaction occurs
with quantitative yield under mild conditions: 1 hour at room temperature. A probable
mechanism of sonochemical isomerization has been proposed. Mono- and poly adducts of
C60 fullerene containing spirocyclic fragments of camphor analogues were obtained in the
reaction of metal complex catalysis by the Pd(acac)2–2PPh3–4Et3Al system with the participation
of fullerene and diazo compounds.
“Cyclophilin A” Enzymatic Effect on the Aggregation Behavior of 1N4R Tau Protein: An Overlooked Crucial Determinant that should be Re-considered in Alzheimer's Disease Pathogenesis
Authors: Ranjbar Samira , Mehrabi Masomeh , Akbari Vali , Pashaei Somayeh , Khodarahmi Reza 
Published: August 2024

Background:
Neurodegenerative disorders like Alzheimer's disease (AD) involve the
abnormal aggregation of tau protein, which forms toxic oligomers and amyloid deposits. The structure
of tau protein is influenced by the conformational states of distinct proline residues, which are
regulated by peptidyl-prolyl isomerases (PPIases). However, there has been no research on the impact
of human cyclophilin A (CypA) as a PPIase on (non-phosphorylated) tau protein aggregation.

Methods:
On the basis of these explanations, we used various spectroscopic techniques to explore
the effects of CypA on tau protein aggregation behavior.

Results:
We demonstrated the role of the isomerization activity of CypA in promoting the formation
of tau protein amyloid fibrils with well-defined and highly ordered cross-β structures. According to
the "cistauosis hypothesis," CypA's ability to enhance tau protein fibril formation in AD is attributed
to the isomerization of specific proline residues from the trans to cis configuration. To corroborate
this theory, we conducted refolding experiments using lysozyme as a model protein. The presence
of CypA increased lysozyme aggregation and impeded its refolding process. It is known that proper
refolding of lysozyme relies on the correct (trans) isomerization of two critical proline residues.

Conclusion:
Thus, our findings confirmed that CypA induces the trans-to-cis isomerization of specific
proline residues, ultimately leading to increased aggregation. Overall, this study highlights the
emerging role of isomerization in tau protein pathogenesis in AD.
Unveiling the Potential of Bergenia Phenolics: Vitexin's Role in Allosteric Modulation of PBP2a as a Strategy against MRSA Resistance
Authors: Verma Abhishek Kumar , Saxena Anshulika , Srivastava Sandeep Kumar 
Published: August 2024

Background:
For cell wall biosynthesis, drug-resistant S. aureus uses a special protein
called PBP2a, even when antibiotics are present and stop its natural processes from working. To
combat this, novel therapies are required to specifically target PBP2a with greater efficacy

Methods:
Using computational approaches, we screened nine phenolic compounds from other
Bergenia species, including Bergenia ciliata, Begenia ligulata, Bergenia purpurascens, and
Bergenia stracheyi, against the PBP2a allosteric site to explore the potential interaction between
phenolic compounds and a specific region of PBP2a known as the allosteric site.

Results:
Based on interaction patterns and estimated affinity, vitexin has been found to be the
most prominent phenolic compound. We performed MD simulations on vitexin and ceftazidime
as control molecules based on the docking results. The binding free energy estimates of vitexin
(-94.48 +/- 17.92 kJ/mol) using MM/PBSA were lower than those of the control (-67.61 +/- 12.29
kJ/mol), which suggests that vitexin may be able to inhibit PBP2a activity in MRSA.

Conclusion:
It has been intriguing to observe a correlation between the affinity of the lead vitexin
at the allosteric site and the modification of Tyr446, the active site gatekeeper residue in PBP2a.
Our findings have implied that lead vitexin can either directly or indirectly decrease PBP2a
activity by inducing allosteric site change in conventional medicine.
Notch Signaling in Central Nervous System: From Cellular Development to Multiple Sclerosis Disease
Authors: Askari Hamid , Rabiei Fatemeh , Yahyazadeh Masoomeh , Biagini Giuseppe , Ghasemi-Kasman Maryam 
Published: August 2024

Introduction/Objective:
Multiple sclerosis (MS), is characterized by autoimmune-driven
neuroinflammation, axonal degeneration, and demyelination. This study aimed to explore the therapeutic
potential of targeting Notch signaling within the central nervous system (CNS) in the context of
MS. Understanding the intricate roles of Notch signaling could pave the way for targeted interventions
to mitigate MS progression.

Methods:
A comprehensive literature review was conducted using databases such as PubMed, Web of
Science, and Scopus. Keywords such as "Notch signaling," "neuroglial interactions," and "MS" were
used. The selection criteria included relevance to neuroglial interactions, peer-reviewed publications,
and studies involving animal models of MS.

Results:
This review highlights the diverse functions of Notch signaling in CNS development, including
its regulation of neural stem cell differentiation into neurons, astrocytes, and oligodendrocytes. In
the context of MS, Notch signaling has emerged as a promising therapeutic target, exhibiting positive
impacts on neuroprotection and remyelination. However, its intricate nature within the CNS necessitates
precise modulation for therapeutic efficacy.

Conclusion:
This study provides a comprehensive overview of the potential therapeutic role of Notch
signaling in MS. The findings underscore the significance of Notch modulation for neuroprotection
and remyelination, emphasizing the need for precision in therapeutic interventions. Further research is
imperative to elucidate the specific underlying mechanisms involved, which will provide a foundation
for targeted therapeutic strategies for the management of MS and related neurodegenerative disorders.
The Quality of Yoghurt Fortification Tuna Fishbone (Thunnus sp.) Nanocalcium: Physicochemistry and Microstructure
Authors: Shodiq Berliana , Lailasari Umi , Prasetiyo Dimas Teguh , Pambudi Ridho , Nurdin Muhammad Alfan 
Published: August 2024

Calcium is very important mineral for human body. Fortification of natural calcium source into yoghurt can be a way to provide calcium requirement. Fortification nanocalcium into yoghurt also can increase bioavailability of calcium. This research aimed to determine the effect of tuna fishbone nanocalcium fortification on total acidity, pH, water holding capacity, viscosity, syneresis, firmness, cohesiveness, particle size and particle distribution of yoghurt. The treatment used were 100 ml yoghurt + 0% nanocalcium (P0), 100 ml yoghurt + 0.12% nanocalcium (P1), 100 ml yoghurt + 0.24% nanocalcium (P2), and 100 ml yoghurt + 0.48% nanocalcium (P3). This research carried out 4 treatments and 3 repetitions. The results showed that the fortification had a significant effect (p < 0.05) on yoghurt's total acidity, pH, water holding capacity. The result of viscosity, firmness and cohesiveness showed the highly significant effect (p < 0.01) in yoghurt and the rest variable result like particle distribution, particle size and syneresis of yoghurt fortification showed no significant effect (p > 0.05). The average result P0; P1; P2; P3 of total acidity () was 1.395±0.085; 1.343±0.0471; 1.272±0.0364; 1.242±0.0469, pH was 4.38 ± 0.04; 4.39 ± 0.01; 4.40 ± 0.03; 4.45 ± 0.01, water holding capacity (%) was 71.17 ±1.90; 71.77±1.96; 74.56 ± 2.2, viscosity (%) was 84.473 ± 0.537; 90.317 ± 0.585; 92.873 ± 1.175; 95.073 ± 0.616, syneresis (%) was 28.140±6.97; 28.790 ±2,19; 27.253 ±4.72; 27.890 ± 4.04, firmness was 42.443 ± 1.093; 45.627 ± 0.994; 47.370 ± 1.295; 54.290 ± 2.509, cohesiveness was 12.679±0.244; -13.180±0.340; -13.913±0.270; -14.492±0.169, particle size (µm) was 9.820±1.950; 8.797±0.120; 8.470±1.869; 8.303±2.442, and particle distribution was 3.027 ±0.01; 2.781 ±0.16; 2.714 ±0.313; and 2.578 ±0.22. The conclusion of this research was fortification of tuna fishbone nanocalcium had effect to decrease total acidity, syneresis, cohesiveness, particle size and particle distribution of yoghurt. Also, the fortification can increase pH, water holding capacity, viscosity and firmness of yoghurt.
Evaluating the Economic Impact of the PedAMINES App in Reducing Medication Errors in Pediatric Emergency Care: Cost-Effectiveness Analysis
Authors: Brunner Loïc , Siebert Johan N , Ehrler Frédéric , Manzano Sergio , Marti Joachim 
Published: August 2024

Background
The administration of drugs in pediatric emergency care is a time-consuming process and is associated with a higher occurrence of medication errors compared with adult care. This is attributed to the intricacies of administration, which involve calculating doses based on the child’s weight or age. To mitigate the occurrence of adverse drug events (ADEs), the PedAMINES (Pediatric Accurate Medication in Emergency Situations; Geneva University Hospitals) mobile app has been developed. This app offers a step-by-step guide for preparing and administering pediatric drugs during emergency interventions by automating the dose calculation process. Although previous simulation-based randomized controlled trials conducted in emergency care have demonstrated the efficacy of the PedAMINES app in reducing drug administration errors, there is a lack of evidence regarding its economic implications.

Objective
This study aims to evaluate the cost-effectiveness of implementing the PedAMINES app for 4 emergency drugs: epinephrine, norepinephrine, dopamine, and midazolam.

Methods
The economic evaluation was conducted by combining hospital data from 2019, previous trial outcomes, information extracted from existing literature, and PedAMINES maintenance costs. The cost per avoided medication error was calculated, along with the number of administrations needed to achieve a positive return on investment. Subsequently, Monte Carlo simulations were used to identify the key parameters contributing to result uncertainty.

Results
The study revealed the number of preventable errors per administration for the 4 examined drugs: 0.513 for epinephrine, 0.484 for norepinephrine, 0.500 for dopamine, and 0.671 for midazolam. The cost-effectiveness ratios per ADE prevented were computed as follows: US $4808 for epinephrine, US $9705 for norepinephrine, US $6957 for dopamine, and US $2074 for midazolam. Accounting for the economic impact of ADEs, the analysis estimated that 16 administrations of epinephrine, 17 of norepinephrine and dopamine, and 13 of midazolam would be required to attain a positive return on investment. This corresponds to roughly one-third of the annual administrations at a major university hospital in Switzerland. The primary factors influencing the uncertainty in the estimated cost per ADE include the cost of maintenance of the app, the likelihood of an ADE resulting from an administration error, and the frequency of underdosing in the trial’s control group.

Conclusions
A dedicated mobile app presents an economically viable solution to alleviate the health and economic burden of drug administration errors in in-hospital pediatric emergency care. The widespread adoption of this app is advocated to pool costs and extend the benefits on a national scale in Switzerland.
Perceptions Toward Using Artificial Intelligence and Technology for Asthma Attack Risk Prediction: Qualitative Exploration of Māori Views
Authors: Jayamini Widana Kankanamge Darsha , Mirza Farhaan , Bidois-Putt Marie-Claire , Naeem M Asif , Chan Amy Hai Yan 
Published: August 2024

Background
Asthma is a significant global health issue, impacting over 500,000 individuals in New Zealand and disproportionately affecting Māori communities in New Zealand, who experience worse asthma symptoms and attacks. Digital technologies, including artificial intelligence (AI) and machine learning (ML) models, are increasingly popular for asthma risk prediction. However, these AI models may underrepresent minority ethnic groups and introduce bias, potentially exacerbating disparities.

Objective
This study aimed to explore the views and perceptions that Māori have toward using AI and ML technologies for asthma self-management, identify key considerations for developing asthma attack risk prediction models, and ensure Māori are represented in ML models without worsening existing health inequities.

Methods
Semistructured interviews were conducted with 20 Māori participants with asthma, 3 male and 17 female, aged 18-76 years. All the interviews were conducted one-on-one, except for 1 interview, which was conducted with 2 participants. Altogether, 10 web-based interviews were conducted, while the rest were kanohi ki te kanohi (face-to-face). A thematic analysis was conducted to identify the themes. Further, sentiment analysis was carried out to identify the sentiments using a pretrained Bidirectional Encoder Representations from Transformers model.

Results
We identified four key themes: (1) concerns about AI use, (2) interest in using technology to support asthma, (3) desired characteristics of AI-based systems, and (4) experience with asthma management and opportunities for technology to improve care. AI was relatively unfamiliar to many participants, and some of them expressed concerns about whether AI technology could be trusted, kanohi ki te kanohi interaction, and inadequate knowledge of AI and technology. These concerns are exacerbated by the Māori experience of colonization. Most of the participants were interested in using technology to support their asthma management, and we gained insights into user preferences regarding computer-based health care applications. Participants discussed their experiences, highlighting problems with health care quality and limited access to resources. They also mentioned the factors that trigger their asthma control level.

Conclusions
The exploration revealed that there is a need for greater information about AI and technology for Māori communities and a need to address trust issues relating to the use of technology. Expectations in relation to computer-based applications for health purposes were expressed. The research outcomes will inform future investigations on AI and technology to enhance the health of people with asthma, in particular those designed for Indigenous populations in New Zealand.
Enhancing Quality of Life in Venous Ulcer Patient with Irsal-e-Alaq (Hirudotherapy): A Case Report
Authors: Siddiqui Saif Ali , Mohammad Sayyed Adnan , Shoaib Mohammad , Sultana Asia 
Published: August 2024

Background: In general practice, venous ulcers (stasis ulcers) are the most common and recurrent leg ulcers. Ignorance of it can have a severe detrimental effect on one's quality of life, cause gangrene, and even the limb amputation. Venous ulcers are the most dangerous and disabling side effect of chronic venous insufficiency. Leech therapy seems to be effective for the treatment of varicose ulcers due to its salivary secretions which it injects during bloodsucking. The saliva of leeches contains certain biochemical with vaso-dilating, anticoagulant, anesthetic, thrombolytic, antibiotic, analgesic, and anti-inflammatory properties. As a result, Irsal-e-Alaq (Hirudotherapy), one of the Unani regimens, can effectively control it. Objective: The purpose of this case report is to provide insight into the effects of Irsal-e-Alaq (Hirudotherapy) on venous ulcers. Intervention: A 60-year-old male has a complaint of ulceration in his left lower leg near the medial malleolus with swelling, pain, discharge, and darkening of the skin of the left lower limb. Four leeches were applied once every week for 2 months. Efficacy of leech therapy was assessed on every 15th day by pain /leg discomfort on 4 point scale ranging from 0-3, limb girth at calf, ankle & foot, pigmentation color on 4 point scale, pigmentation area, pain in ulcer on 4 point scale from 0-3, ulcer depth, PUSH Score. Hb% was assessed every 15th day to check anemia, colour flow Doppler was done to assess any change in vein anatomy. Result: In this case report, the ulcers were completely healed, and also showed remarkable improvement in other variables. Conclusion: Irsal-e-Alaq has been proven beneficial in treating venous ulcers and enhancing patient’s quality of life.
Keywords: Venous ulcer, Hirudotherapy, Unani Medicine, Pain, Pigmentation.
Phenotypic characterization and antibiotic resistance of enterobacteria strains isolated from samples of patients in the towns of Moundou and Sarh in Chad
Authors: Gamougam Kadidja , Djibrine Mayoré Atéba , Hissein Abdoulahhi Ousman , Tidjani Abdelsalam 
Published: August 2024

Today, enterobacteria constitute one of the most predominant causes of nosocomial and acquired infections in our communities. The bactericidal action of antibiotics on bacteria is used therapeutically, unfortunately the latter have started to develop resistances. Antibiotic resistance remains a major global public health issue, a serious problem in some of the world's poorest countries such as Chad.
The objective of this study is to identify the strains of enterobacteria coming from samples of patients admitted to the Moundou and Sarh health centers, and to report their resistance profiles. A total of 278 samples consisting of urine (133) and stools (145) were collected in the laboratories of the two provincial hospitals of Sarh and Moundou between September and December 2021. The samples were processed and analyzed according to standard microbiology methods. The study of the sensitivity of different strains of enterobacteria with 14 antibiotic disks was evaluated by the disk diffusion method in agar medium.
A total of 278 samples (urine, stool) including 111 strains of Enterobacteria, including 55 strains of E. coli, Enterobacter spp (n=17), klebsiella (n=11), Salmonella (n=9), Serratia ( n=7), Citrobacter (n=6) and Shigella (n=2) were isolated in the laboratory in the towns of Moundou and Sarh in Chad. The antibiogram carried out on all isolated strains of enterobacteria expressed a resistance ranging between 33.3% and 83.3% to 3rd generation cephalosporins, 30.3% and 50.0% to aminoglycosides. On the other hand, a strain of Salmonella is resistant to imipenem in Sarh.
This study shows a high level of resistance acquired to different families of antibiotics by our bacterial strains studied. This resistance highlights the need to adapt therapeutic regimens to local epidemiology.
Keywords : Characterization, Phenotype, Antibiotic resistance, Enterobacteria, Cities, Chad
The Influence of Students' Attribute on Earthquake Understanding in Klaten Regency
Authors: Dewi Ratih Puspita , Musiyam Muhammad , Widiyatmoko Wahyu , Wibowo Yunus Aris , Suharjo Suharjo , Miftakhunisa Yunita , Khoirunnisa Hakimah 
Published: August 2024

An earthquake is a type of natural disaster that cannot be predicted in terms of time or scale. Klaten Regency is one of the earthquake-prone areas of Indonesia. The occurrence of earthquakes in Klaten Regency has indeed surprised many parties. Implementing disaster mitigation measures is crucial in areas prone to disasters. Disaster mitigation education has been implemented in schools through intracurricular and extracurricular activities. This study aims to analyze (1) the student's understanding of earthquakes at the elementary, middle, and high school levels in Klaten Regency and (2) the impact of student attributes on an understanding of earthquakes. Students' Students comprise age and gender. This is a quantitative study with a correlational research approach. The study was 173 students from junior high school. Techniques for collecting data include questionnaires and documentation. Data analysis techniques employ descriptive statistics; furthermore, the tests used to answer the hypothesis are the Mann-Whitney test, kruskall Wallis, and Spearman rank. The Mann-Whitney test. According to the study results, (1) students' students' of earthquakes was 22% high, 75% moderate, and 3% low, and (2) The test results pointed out that student characteristics had no significant impact on students' student's knowledge.Keywords:Â Earthquake; Disaster; Students; Understanding of Disaster