bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Diversity among Pentaploid Buffelgrass Accessions
Authors: Burson Byron L. , Renganayaki K. , Dowling Charlie D. , Hinze Lori L. , Jessup Russell W. 
Published: June 2015

ABSTRACTBuffelgrass [Pennisetum ciliare (L.) Link (syn. Cenchrus ciliaris L.)], an important pasture and range grass in the arid semi‐tropics and tropics, has excellent drought‐tolerance but lacks winter hardiness. It is a polymorphic, apomictic species and its most common chromosome number is 2n = 4x = 36. Eighty‐six pentaploid (2n = 5x = 45) accessions from South Africa were deposited into the USDA National Germplasm System (NPGS) in 1976 and these were more winter‐hardy than all tetraploid accessions. Prior to 1976, only 31 pentaploids were in the NPGS but they lacked cold tolerance. Since 1976, ten pentaploid accessions with unknown cold tolerance have been added to the NPGS and they were included. The genetic diversity among all these pentaploid accessions was investigated to determine if the winter‐hardy and non‐winter‐hardy genotypes were distinct. Amplified fragment length polymorphisms were used to determine the genetic diversity and phylogenetic relationships among the accessions. Ten primer combinations generated 862 polymorphic bands; the number of bands identified by each primer ranged from 74 to 117 (mean 93.4). Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis and principal coordinate analysis revealed two groups with one separating into two sub‐groups and one sub‐group separating into two subdivisions. Genetic similarity coefficients ranged from 0.39 to 0.98 (mean 0.74). These findings show considerable genetic diversity among the pentaploid accessions. Most cold‐tolerant and non‐cold‐tolerant genotypes separated by subgroups but not by group. Nine tetraploid accessions, included for reference, clustered into several subgroups. The pentaploid genotypes appear to have polyphyletic origins.
Reduced Nitrogen Mineralization and Litter Decomposition in Unfertilized Smooth Bromegrass Pastures
Authors: Wingeyer Ana B. , Guretzky John A. , Schacht Walter H. , Klopfenstein Terry J. 
Published: June 2015

ABSTRACTNitrogen fertilization has been shown to affect herbage accumulation, litter deposition, litter N return, and presence of weeds in smooth bromegrass (Bromus inermis Leyss.) pastures. The objective of this study was to evaluate the effect of pasture management strategies that varied amount and form of N input on soil N mineralization, litter decomposition, and litter N release in smooth bromegrass pasture. Management strategies included: (i) nitrogen‐fertilized pasture grazed with unsupplemented beef cattle (FERT); (ii) unfertilized pasture grazed with unsupplemented beef cattle (CONT); and (iii) unfertilized pasture grazed with dried distillers grains plus solubles (DDGS)‐supplemented beef cattle (SUPP). After 210 d of aerobic soil incubation, cumulative soil N mineralization was 155.1 ± 9.8, 170.5 ± 15.6, and 180.2 ± 13.8 mg N kg–1 soil for CONT, SUPP, and FERT pastures, respectively, which represented a supply of 254, 279, and 294 kg N ha–1 for the 0‐ to 15‐cm soil depth, respectively. Decomposition day models incorporating precipitation and temperature generally were the best predictors of litter decomposition. Regardless of the model used, there was no difference in litter decomposition among management strategies in 2010, while in 2011, both leaf and stem litter decomposed faster in FERT than CONT and SUPP pastures. After 8 yr of management, cessation of N fertilization in CONT and SUPP resulted in a soil environment that supported less litter decomposition than FERT.
Focusing on the “T” in LGBT: An Online Survey of Related Content in Texas Nursing Programs
Authors: Walsh David , Hendrickson Sherry Garrett 
Published: June 2015

Background:
As nurses, we advocate for the most vulnerable and underserved, who, within the lesbian, gay, bisexual, and transgender (LGBT) community, are transgender individuals. Yet, the existence of LGBT education in nursing schools has not been examined.

Method:
After approval by the university institutional review board, 113 nursing programs in Texas were surveyed between November 2013 and January 2014, with a 12-question, Web-based questionnaire. A Verisign certificate and 128-bit encryption program supported compliance with the Health Insurance Portability and Accountability Act of 1996.

Results:
Nineteen percent of the surveys were returned. Ten (47.62%) of 21 respondents addressed transgender or transsexual individuals. Fifteen (71.43%) of 21 answered a free-text question to estimate the number of hours spent addressing LGBT content, reporting an average of 1.6 hours.

Conclusion:

Our study suggests that, in Texas, nursing students may not be receiving sufficient content, nor do they understand transgender health needs or how to best deliver competent, compassionate care to this population. [
J Nurs Educ
. 2015;54(6):347–351.]
How does increasing horizontal resolution in a global climate model improve the simulation of aerosol‐cloud interactions?
Authors: Ma Po‐Lun , Rasch Philip J. , Wang Minghuai , Wang Hailong , Ghan Steven J. , Easter Richard C. , Gustafson William I. , Liu Xiaohong , Zhang Yuying , Ma Hsi‐Yen 
Published: June 2015

AbstractThe Community Atmosphere Model Version 5 is run at horizontal grid spacing of 2, 1, 0.5, and 0.25°, with the meteorology nudged toward the Year Of Tropical Convection analysis, and cloud simulators and the collocated A‐Train satellite observations are used to explore the resolution dependence of aerosol‐cloud interactions. The higher‐resolution model produces results that agree better with observations, showing an increase of susceptibility of cloud droplet size, indicating a stronger first aerosol indirect forcing (AIF), and a decrease of susceptibility of precipitation probability, suggesting a weaker second AIF. The resolution sensitivities of AIF are attributed to those of droplet nucleation and precipitation parameterizations. The annual average AIF in the Northern Hemisphere midlatitudes (where most anthropogenic emissions occur) in the 0.25° model is reduced by about 1 W m−2 (−30%) compared to the 2° model, leading to a 0.26 W m−2 reduction (−15%) in the global annual average AIF.
Research on the change of chemical composition in productive process of Re Du Ning injection by HPLC/Q‐TOF MS
Authors: Zhang Shan , Li Yan‐Jing , Zhang Chun‐Xiao , Huang Wen‐Zhe , Ding Gang , Wang Zhen‐Zhong , Bi Yu‐An , Xiao Wei 
Published: June 2015

AbstractA high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (HPLC/Q‐TOF MS) was developed for the analysis of chemical composition change in the production process of Re Du Ning injection, a Chinese medicine preparation with a combination of Lonicera japonica Thunb., Gardenia jasminoides Ellis and Artemisia annua L. A total of 90 compounds from raw materials–intermediates–Re Du Ning injection were detected; among them, 55 compounds were identified or tentatively characterized, and the characteristic ions of different types of compounds were described. Based on these studies, the different types of compounds in the various process routes were analyzed. A total of 28 compounds, including seven iridoid glycosides and six monoterpenes from G. jasminoides Ellis, five iridoid glycosides, nine phenolic acids and one unknown compound from L. japonica Thunb., were transferred to Re Du Ning injection, and two unknown compounds were generated in the production process of Re Du Ning injection. The results indicated that the Chinese Medicine Pharmaceutical process control is very important. This method could provide some reference for other Chinese medicine preparations. Copyright © 2015 John Wiley & Sons, Ltd.
Hydrological and sedimentological processes of flood layer formation in Lake Mondsee
Authors: Kämpf Lucas , Mueller Philip , Höllerer Hannes , Plessen Birgit , Naumann Rudolf , Thoss Heiko , Güntner Andreas , Merz Bruno , Brauer Achim 
Published: June 2015

AbstractDetrital layers in lake sediments are recorders of extreme flood events. However, their use for establishing time series of past floods is limited by lack in understanding processes of detrital layer formation. Therefore, we monitored hydro‐sedimentary dynamics in Lake Mondsee (Upper Austria) and its main tributary, Griesler Ache, over a 3‐year period from January 2011 to December 2013. Precipitation, discharge and turbidity were recorded continuously at the river outlet to the lake and compared to sediment fluxes trapped with 3 to 12 days resolution at two locations in the lake basin, in a distance of 0·9 (proximal) and 2·8 km (distal) to the Griesler Ache inflow. Within the 3‐year observation period, 26 river floods of different magnitude (10 to 110 m3 s−1) have been recorded resulting in variable sediment fluxes to the lake (4 to 760 g m−2 d−1) including the ‘century‐scale’ flood event in June 2013. The comparison of hydrological and sedimentological data revealed (i) a rapid sedimentation within 3 days after the peak runoff in the proximal and within 6 to 10 days in the distal lake basin; (ii) empirical flood thresholds for triggering sediment flux at the lake floor increasing from the proximal (20 m3 s−1) to the distal lake basin (30 m3 s−1) and (iii) various factors that control the detrital sediment transport in the lake. The amount of sediment transported to the lake is controlled by runoff and catchment sediment availability. The distribution of detrital sediment within the lake basin is mainly driven by mesopycnal interflows and closely linked to flood duration and the season in which a flood occurred. The combined hydro‐sedimentary monitoring revealed detailed insights into processes of flood layer formation in a meso‐scale peri‐Alpine lake and, thereby, improves the interpretation of the depositional record of flood layers.
Hydro‐morphodynamic evolution in a 90° movable bed discordant confluence with low discharge ratio
Authors: Guillén‐Ludeña Sebastián , Franca Mário J. , Cardoso António H. , Schleiss Anton J. 
Published: June 2015

AbstractConfluences with low discharge and momentum ratios, where narrow steep tributaries with high sediment load join a wide low‐gradient main channel that provides the main discharge, are often observed in high mountain regions such as in the upper‐Rhone river catchment in Switzerland. Few existing studies have examined the hydro‐morphodynamics of this type of river confluence while considering sediment discharge in both confluent channels. This paper presents the evolution of the bed morphology and hydrodynamics as observed in an experimental facility with a movable bed. For that purpose, one experiment was carried out in a laboratory confluence with low discharge and momentum ratios, where constant sediment rates were supplied to both flumes. During the experiment, bed topography and water surface elevations were systematically recorded. When the bed topography reached a steady state (so‐called equilibrium) and the outgoing sediment rate approximated the incoming rate, flow velocity was measured at 12 different points distributed throughout the confluence, and the grain size distribution of the bed surface was analyzed. Typical morphodynamic features of discordant confluences such as a bank‐attached bar and a flow deflection zone are identified in this study. Nevertheless, the presence of a marked scour hole in the discordant confluence and distinct flow regimes for the tributary and main channel, differ from results obtained in previous studies. Strong acceleration of the flow along the outer bank of the main channel is responsible for the scour hole. This erosion is facilitated by the sediment discharge into the confluence from the main channel which inhibits bed armoring in this region. The supercritical flow regime observed in the tributary is the hydrodynamic response to the imposed sediment rate in the tributary. Copyright © 2015 John Wiley & Sons, Ltd.
Intravenous Bisphosphonate Therapy of Young Children With Osteogenesis Imperfecta: Skeletal Findings During Follow Up Throughout the Growing Years
Authors: Palomo Telma , Fassier François , Ouellet Jean , Sato Atsuko , Montpetit Kathleen , Glorieux Francis H , Rauch Frank 
Published: June 2015

ABSTRACT

Cyclical intravenous bisphosphonate therapy is widely used to treat children with osteogenesis imperfecta (OI), but little is known about long-term treatment outcomes. We therefore reviewed 37 children with OI (OI type I, n = 1; OI type III, n = 14; and OI type IV, n = 22) who started intravenous bisphosphonate therapy before 5 years of age (median 2.2 years; range, 0.1 to 4.8 years), and who had a subsequent follow-up period of at least 10 years (median 14.8 years; range, 10.7 to 18.2 years), during which they had received intravenous bisphosphonate treatment (pamidronate or zoledronic acid) for at least 6 years. During the observation period, the mean lumbar spine areal bone mineral density Z-score increased from –6.6 (SD 3.1) to –3.0 (SD 1.8), and weight Z-score increased from –2.3 (SD 1.5) to –1.7 (SD 1.7) (p &lt; 0.001 and p = 0.008). At the time of the last assessment, patients with OI type IV had significantly higher height Z-scores than a control group of patients matched for age, gender, and OI type who had not received bisphosphonates. Patients had a median of six femur fractures (range, 0 to 18) and five tibia fractures (range, 0 to 17) during the follow-up period. At baseline, 35% of vertebra were affected by compression fractures, whereas only 6% of vertebra appeared compressed at the last evaluation (p &lt; 0.001), indicating vertebral reshaping during growth. Spinal fusion surgery was performed in 16 patients (43%). Among the 21 patients who did not have spinal fusion surgery, 13 had scoliosis with a curvature ranging from 10 to 56 degrees. In conclusion, long-term intravenous bisphosphonate therapy was associated with higher Z-scores for lumbar spine areal bone mineral density and vertebral reshaping, but long-bone fracture rates were still high and the majority of patients developed scoliosis. © 2015 American Society for Bone and Mineral Research.
Selenoprotein S Is Highly Expressed in the Blood Vessels and Prevents Vascular Smooth Muscle Cells From Apoptosis
Authors: Ye Yali , Fu Fen , Li Xiaoming , Yang Jie , Liu Hongmei 
Published: June 2015

ABSTRACTAtherosclerosis and related cardiovascular diseases (CVD) represent one of the greatest threats to human health worldwide. The protection of vascular smooth muscle cells (VSMCs) from apoptosis in the plaque has become an important therapeutic target for atherosclerotic plaque stabilization. A significant association of selenoprotein S (SelS) gene polymorphism with atherosclerotic CVD has been reported in epidemiologic studies, but the underlying mechanism remains unknown. In this paper, SelS expression in the thoracic aorta and its role in the protection of VSMCs from apoptosis have been studied. Western blot analysis showed that SelS was highly expressed in rat thoracic aorta. SelS gene silence by small interference RNA (siRNA) rendered VSMCs more sensitive to hydrogen peroxide‐ or tunicamycin‐ induced injury and apoptosis, as determined by MTT assay, Hoechst staining, and annexin V/propidium iodide staining. SelS silence aggravated hydrogen peroxide‐induced oxidative stress and phosphorylation of p38 MAPK and c‐Jun N‐terminal kinase (JNK) in VSMCs. Furthermore, SelS silence enhanced endoplasmic reticulum (ER) stress induced by hydrogen peroxide or tunicamycin, as showed by the increased protein levels of ER chaperone 78 kDa glucose‐regulated protein (GRP78), ER stress transducer phosphorylated protein kinase RNA like ER kinase (PERK), and the proapoptotic transcription factor C/EBP homologous protein (CHOP). In conclusion, the present study suggested that SelS highly expressed in the blood vessel might protect VSMCs from apoptosis by inhibiting oxidative stress and ER stress. Our finding provided mechanistic insights for the potential preventive role of SelS in atherosclerotic CVD. J. Cell. Biochem. 117: 106–117, 2016. © 2015 Wiley Periodicals, Inc.
Uterine leiomyoma confounding a noninvasive prenatal test result
Authors: Dharajiya Nilesh G. , Namba Akira , Horiuchi Isao , Miyai Shunsuke , Farkas Daniel H. , Almasri Eyad , Saldivar Juan‐Sebastian , Takagi Kenjiro , Kamei Yoshimasa 
Published: June 2015

What's already known about this topic?

The noninvasive prenatal testing from maternal blood can be confounded by maternal conditions that lead to structural variants/copy number variations in the genome.
What does this study add?

This study provides further evidence that the grossly ‘abnormal’ and ‘non‐reportable’ noninvasive prenatal testing result due to global genomic changes was attributable to a maternal uterine leiomyoma, in an otherwise normal fetus. The possibility of such adventitious findings is something about which physicians ordering noninvasive prenatal testing for their patients may want to offer pre‐test counseling.
© 2015 John Wiley & Sons, Ltd.
Dynamic Proteomic Profiling of Extra-Embryonic Endoderm Differentiation in Mouse Embryonic Stem Cells
Authors: Mulvey Claire M. , Schröter Christian , Gatto Laurent , Dikicioglu Duygu , Fidaner Isik Baris , Christoforou Andy , Deery Michael J. , Cho Lily T. Y. , Niakan Kathy K. , Martinez-Arias Alfonso , Lilley Kathryn S. 
Published: June 2015

Abstract
During mammalian preimplantation development, the cells of the blastocyst's inner cell mass differentiate into the epiblast and primitive endoderm lineages, which give rise to the fetus and extra-embryonic tissues, respectively. Extra-embryonic endoderm (XEN) differentiation can be modeled in vitro by induced expression of GATA transcription factors in mouse embryonic stem cells. Here, we use this GATA-inducible system to quantitatively monitor the dynamics of global proteomic changes during the early stages of this differentiation event and also investigate the fully differentiated phenotype, as represented by embryo-derived XEN cells. Using mass spectrometry-based quantitative proteomic profiling with multivariate data analysis tools, we reproducibly quantified 2,336 proteins across three biological replicates and have identified clusters of proteins characterized by distinct, dynamic temporal abundance profiles. We first used this approach to highlight novel marker candidates of the pluripotent state and XEN differentiation. Through functional annotation enrichment analysis, we have shown that the downregulation of chromatin-modifying enzymes, the reorganization of membrane trafficking machinery, and the breakdown of cell–cell adhesion are successive steps of the extra-embryonic differentiation process. Thus, applying a range of sophisticated clustering approaches to a time-resolved proteomic dataset has allowed the elucidation of complex biological processes which characterize stem cell differentiation and could establish a general paradigm for the investigation of these processes. Stem Cells 2015;33:2712—2725
A shortest path method for sequential change propagations in complex engineering design processes
Authors: Li Yuliang , Zhao Wei , Ma Yongsheng 
Published: June 2015

AbstractEngineering design changes constantly occur in complex engineering design processes. Designers need appropriate measures to handle the numerous design changes in order to realize consistent and completely validated product models so that successful product development is assured. In this paper, a time-based mathematic model is presented to characterize the sequential change propagation process, and then the shortest path algorithm is given to find the most timesaving routes for changes to propagate to other dependent design tasks. An analysis method is introduced to compute the sensitivities of change impacts on the affected design tasks, which indicates that the more time consumed by a change to take its effect, the more sensitive the change impacts on those downstream dependent tasks. A case study of change propagations in motorcycle engine design process was presented to demonstrate the proposed method.
Energy efficiency, robustness, and makespan optimality in job-shop scheduling problems
Authors: Salido Miguel A. , Escamilla Joan , Barber Federico , Giret Adriana , Tang Dunbing , Dai Min 
Published: June 2015

AbstractMany real-world problems are known as planning and scheduling problems, where resources must be allocated so as to optimize overall performance objectives. The traditional scheduling models consider performance indicators such as processing time, cost, and quality as optimization objectives. However, most of them do not take into account energy consumption and robustness. We focus our attention in a job-shop scheduling problem where machines can work at different speeds. It represents an extension of the classical job-shop scheduling problem, where each operation has to be executed by one machine and this machine can work at different speeds. The main goal of the paper is focused on the analysis of three important objectives (energy efficiency, robustness, and makespan) and the relationship among them. We present some analytical formulas to estimate the ratio/relationship between these parameters. It can be observed that there exists a clear relationship between robustness and energy efficiency and a clear trade-off between robustness/energy efficiency and makespan. It represents an advance in the state of the art of production scheduling, so obtaining energy-efficient solutions also supposes obtaining robust solutions, and vice versa.
Mobile technology in second language classrooms: Insights into its uses, pedagogical implications, and teacher beliefs
Authors: Van Praag Benjamin , Sanchez Hugo Santiago 
Published: June 2015

AbstractAdopting a multiple-case, multiple-method design, this study investigates mobile technology use in the practices of three experienced second language teachers. The findings, based on an analysis of the teachers’ rationales, stated beliefs and classroom actions, show that the teachers tend to prohibit or reluctantly tolerate mobile device usage, while they recognise some of its potential benefits to support their teaching and student learning. They also highlight the incentives and barriers which respectively facilitate and hinder the integration of mobile technology, including factors which are internal (e.g. beliefs) and external (e.g. contextual constraints) to the teachers. Implications for classroom practice and teacher education are drawn.
Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences
Authors: Shanmugam Raghuvaran , Fierer Jacob , Kaiser Steffen , Helm Mark , Jurkowski Tomasz P , Jeltsch Albert 
Published: June 2015

AbstractThe Dnmt2 RNA methyltransferase catalyses the methylation of C38 in the anticodon loop of tRNA-Asp, but the molecular role of this methylation is unknown. Here, we report that mouse aspartyl-tRNA synthetase shows a four to fivefold preference for C38-methylated tRNA-Asp. Consistently, a 30% reduced charging level of tRNA-Asp was observed in Dnmt2 knockout (KO) murine embryonic fibroblast cells. Gene expression analysis with fluorescent reporter proteins fused to an N-terminal poly-Asp sequence showed that protein synthesis of poly-Asp-tagged reporter proteins was reduced in Dnmt2 KO cells as well. The same effect was observed with endogenous proteins containing poly-Asp sequences, indicating that Dnmt2-mediated C38 methylation of tRNA-Asp regulates the translation of proteins containing poly-Asp sequences. Gene ontology searches for proteins containing poly-Asp sequences in the human proteome showed that a significant number of these proteins have roles in transcriptional regulation and gene expression. Hence, the Dnmt2-mediated methylation of tRNA-Asp exhibits a post-transcriptional regulatory role by controlling the synthesis of a group of target proteins containing poly-Asp sequences.