bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abdominal Cardiac Devices in Pediatric and Congenital Heart Disease
Authors: LaPage Martin J. , Dechert Brynn E. , Hirsch-Romano Jennifer C. , Bradley David J. 
Published: April 2015

Background: Abdominal cardiac implantable electronic device maintenance procedures are traditionally performed by cardiac surgeons. The University of Michigan Congenital Heart Center transitioned from this practice model to one in which electrophysiologists perform the majority of these procedures. This study presents the outcomes of these procedures during this transition. Methods: Retrospective cohort study of all patients undergoing abdominal device maintenance procedure, inclusive of generator change, removal, or placement with preexisting leads from January 2005 to July 2013. Procedures involving epicardial lead placement were excluded. The primary outcome was major complications defined as any intraoperative complication, the requirement of an additional operative intervention, or hospitalization for device-related infection. Results: There were 113 procedures on 93 patients. Of these, 84 (74%) procedures were on patients with congenital heart disease. Cardiac surgeons performed 54 (48%) procedures and electrophysiologists performed 59 (52%). Mean age was 16 ± 11 years. The groups were similar regarding age and proportion with congenital heart disease (CHD). Major complications occurred in 3 (5.5%) cardiac surgeon procedures and 2 (3.4%) electrophysiologist procedures. There is no difference in the risk of major complications between groups ( P = .59). Conclusion: This 8.5-year period encompassed a practice model transition from cardiac surgeon-performed abdominal device procedures to primarily electrophysiologist-performed abdominal device procedures. There was no difference in the risk of complications between services. This suggests that electrophysiologist-performed abdominal cardiac device maintenance procedures are a viable practice model, provided there is support and collaboration from the cardiac surgery service.
Faster T-cell development following gene therapy compared with haploidentical HSCT in the treatment of SCID-X1
Authors: Touzot Fabien , Moshous Despina , Creidy Rita , Neven Bénédicte , Frange Pierre , Cros Guilhem , Caccavelli Laure , Blondeau Johanna , Magnani Alessandra , Luby Jean-Marc , Ternaux Brigitte , Picard Capucine , Blanche Stéphane , Fischer Alain , Hacein-Bey-Abina Salima , Cavazzana Marina 
Published: April 2015

Key Points
SCID-X1 patients treated with gene therapy show faster T-cell development compared with patients treated with haploidentical HSCT.
Association of HLA-B*41:02 with Henoch-Schönlein Purpura (IgA Vasculitis) in Spanish individuals irrespective of the HLA-DRB1 status
Authors: López-Mejías Raquel , Genre Fernanda , Pérez Belén Sevilla , Castañeda Santos , Ortego-Centeno Norberto , Llorca Javier , Ubilla Begoña , Remuzgo-Martínez Sara , Mijares Verónica , Pina Trinitario , Calvo-Río Vanesa , Márquez Ana , Miranda-Filloy José A , Parejo Antonio Navas , Conde-Jaldón Marta , Ortiz-Fernández Lourdes , Argila Diego , Aragües Maximiliano , Rubio Esteban , León Luque Manuel , Blanco-Madrigal Juan María , Galíndez-Aguirregoikoa Eva , Escribano Francisca González , Ocejo-Vinyals J Gonzalo , Martín Javier , Blanco Ricardo , González-Gay Miguel A 
Published: April 2015

Abstract
Introduction
A study was conducted to determine whether the human leukocyte antigen (HLA) B alleles are implicated in the susceptibility to Henoch-Schönlein purpura (HSP) in the largest series of Caucasian HSP patients ever assessed for genetic studies.

Methods
The study population was composed of 349 Spanish patients diagnosed with HSP fulfilling the American College of Rheumatology and the Michel et al. classification criteria, and 335 sex and ethnically matched controls. HLA-B phenotypes were determined by sequencing-based typing (SBT) and analyzed by chi-square or Fisher exact test.

Results
A statistically significant increase of HLA-B*41:02 allele in HSP patients when compared with controls was found (8.3% versus 1.5% respectively; P = 0.0001; OR (odds ratio) =5.76 [2.15-19.3]). These results remained statistically significant after adjusting for Bonferroni correction (P = 0.0028). An internal validation also confirmed the susceptibility effect on HSP associated with HLA-B*41:02 (OR = 5.70 [1.98-16.44]). Since a former study described an association between HLA-DRB1*01:03 and HSP susceptibility, we also evaluated the implication of HLA-B*41:02 independently of HLA-DRB1*01:03. Interestingly, the association remained statistically significant (P = 0.0004, OR = 4.97 [1.8-16.9]). No HLA-B association with specific HSP clinical features was found.

Conclusions
Our study indicates that HLA-B*41:02 is associated with the susceptibility to HSP in Spanish patients irrespective of HLA-DRB1 status.
Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation
Authors: Paula Ana CC , Martins Thaís MM , Zonari Alessandra , Frade Soraia PPJ , Angelo Patrícia C , Gomes Dawidson A , Goes Alfredo M 
Published: April 2015

Abstract
Introduction
Human adipose tissue-derived stem cells (hASCs) are attractive cells for therapeutic applications and are currently being evaluated in multiple clinical trials. Prior to their clinical application, hASCs must be expanded ex vivo to obtain the required number of cells for transplantation. Fetal bovine serum is the supplement most widely used for cell culture, but it has disadvantages and it is not safe for cell therapy due to the risks of pathogen transmission and immune reaction. Furthermore, the cell expansion poses a risk of accumulating genetic abnormalities that could lead to malignant cell transformation. In this study, our aim was to evaluate the proliferation pattern as well as the resistance to spontaneous transformation of hASCs during expansion in a xeno-free culture condition.

Methods
hASCs were expanded in Dulbecco’s modified Eagle’s medium supplemented with pooled allogeneic human serum or fetal bovine serum to enable a side-by-side comparison. Cell viability and differentiation capacity toward the mesenchymal lineages were assessed, along with immunophenotype. Ki-67 expression and the proliferation kinetics were investigated. The expression of the transcription factors c-FOS and c-MYC was examined with Western blot, and MYC, CDKN2A, ERBB2 and TERT gene expression was assessed with quantitative PCR. Senescence was evaluated by β-gal staining. Karyotype analysis was performed and tumorigenesis assay in vivo was also evaluated.

Results
The hASCs expanded in medium with pooled allogeneic human serum did not show remarkable differences in morphology, viability, differentiation capacity or immunophenotype. The main difference observed was a significantly higher proliferative effect on hASCs cultured in pooled allogeneic human serum. There was no significant difference in C-FOS expression; however, C-MYC protein expression was enhanced in pooled allogeneic human serum cultures compared to fetal bovine serum cultures. No difference was observed in MYC and TERT mRNA levels. Moreover, the hASCs presented normal karyotype undergoing senescence, and did not form in vivo tumors, eliminating the possibility that spontaneous immortalization of hASCs had occurred with pooled allogeneic human serum.

Conclusions
This complete characterization of hASCs cultivated in pooled allogeneic human serum, a suitable xeno-free approach, shows that pooled allogeneic human serum provides a high proliferation rate, which can be attributed for the first time to C-MYC protein expression, and showed cell stability for safe clinical applications in compliance with good manufacturing practice.
Effect of Cooking Temperature on Mineral Content and Anti-nutritional Factors of Yam and Taro Grown in Southern Ethiopia
Authors: Ayele Esayas , Urga Kelbessa , Chandravanshi Bhagwan Singh 
Published: April 2015

Abstract
This study aimed to determine the mineral composition and anti-nutritional factors, oxalate and phytate, of yam and taro grown in southern Ethiopia. In addition, the effect of cooking at different temperatures on the mineral compositions and anti-nutritional factors together with respective molar ratios Ca:oxalate, Zn:phytate, Ca:phytate and (Ca × phytate):Zn was determined. The mineral compositions were found to be: 21.8–15.7, Ca; 11.4–12.1, Na; 7.02–7.24, Mg; 6.47–14.14, Zn; 2.04–4.3, Fe; 0.26–0.88, Mn; 0.50–0.60, Cu; 0.57–0.82, Co; and 0.20–2.15, Ni in mg/100 g in the raw yam samples and 41–53, Ca; 6.21–7.21, Na; 7.32–7.58, Mg; 42–50, Zn; 1.93–3.25, Fe; 1.71–5.88, Mn; 0.46–0.51, Cu; 0.65–0.95, Co; and 0.18–0.68 Ni in mg/100 g of raw taro samples. Mineral content decreased by cooking except Fe and Ca in one variety of taro where they show a bit increment. The anti-nutritional factor to mineral ratio tends to imply that the relative bioavailability of the minerals after cooking was found to be increased except for that of Fe. These tubers may present health hazard potential, which in turn demands proper processing before consumption to eliminate the toxic effects of anti-nutritional factors.
Children’s Insurance Coverage and Crowd-Out Through the Recession: Lessons From Ohio
Authors: Muhlestein David , Seiber Eric 
Published: April 2015

Objectives. We estimated changes in children’s insurance status (publicly insured, privately insured, or uninsured) and crowd-out rates during the 2007 to 2009 US recession in Ohio. Methods. We conducted an estimate of insurance coverage from statewide, randomized telephone surveys in 2004, 2008, 2010, and 2012. We estimated crowd-out by using regression discontinuity. Results. From 2004 to 2012, private insurance rates dropped from 67% to 55% and public rates grew from 28% to 40%, with no change in the uninsured rate for children. Despite a 12.0% decline in private coverage and a corresponding 12.6% increase in public coverage, we found no evidence that crowd-out increased during this period. Conclusions. Children, particularly those with household incomes lower than 400% of the federal poverty level, were enrolled increasingly in public insurance rather than private coverage. Near the Medicaid eligibility threshold, this is not from an increase in crowd-out. An alternative explanation for the increase in public coverage would be the decline in incomes for households with children.
Effects of Crosslinking Chemistry on Proppant Suspension in Guar Networks
Authors: Hu Y. Thomas , Kishore Tejaswini , Maxey Jason , Loveless David 
Published: April 2015

Abstract
Dynamic proppant suspension behavior of carboxymethyl hydroxypropyl guar (CMHPG) crosslinked with labile (reversible) and inert (irreversible) crosslinkers are quantified and compared in this work. In reversibly crosslinked CMHPG by borate, particle settling slows with increased imposed orthogonal shear rate and proppants remain suspended for long periods above a critical shear rate. On the contrary, in irreversible zirconium crosslinked CMHPG, particle settling is negligible at low shear rates, dramatically accelerating above a critical shear rate. Local flow field data was obtained to provide insight into the dramatically different particle settling behavior. It was observed that the slowing of particle settling with increased shear rate in borate crosslinked CMHPG originates from shear-induced gelation, which leads to significant increases to both viscosity and elastic stresses. For zirconium crosslinked CMHPG sheared at low shear rates, the local velocity profile is flat, indicating that the network remains intact with negligible flow in the bulk. Above the critical shear rate, the velocity profile becomes sloped, indicating breakup of the network. Therefore, reversibly crosslinked polymers suspend proppant better at high shear rates with an upper critical shear rate for proppant suspension, whereas irreversibly crosslinked polymers suspend proppant better at low shear rates with a lower critical shear rate for proppant suspension. These findings provide valuable guidance in terms of optimizing fracture fluid design to help maximize proppant transport into fractures.
Measuring Change in Somatosensation Across the Lifespan
Authors: Dunn Winnie , Griffith James W. , Sabata Dory , Morrison Mary T. , MacDermid Joy C. , Darragh Amy , Schaaf Roseann , Dudgeon Brian , Connor Lisa Tabor , Carey Leeanne , Tanquary Jennifer 
Published: April 2015

Abstract
OBJECTIVE. The study aim was to determine natural variability in somatosensation across age groups using brief measures. We validated measures in a community-dwelling population as part of the National Institutes of Health (NIH) Toolbox for Assessment of Neurological and Behavioral Function (NIH Toolbox; http://www.nihtoolbox.org).
METHOD. Participants included community-dwelling children and adults (N = 367, ages 3–85 yr) across seven sites. We tested haptic recognition, touch detection–discrimination, and proprioception using brief affordable measures as required by the NIH Toolbox.
RESULTS. Accuracy improved from young children to young adults; from young to older adults, the pattern reversed slightly. We found significant differences between adults and older adults. One proprioception test (kinesthesia; p =.003) showed gender differences (females more accurate). We provide expected score ranges for age groups as a basis for understanding age-related expectations for somatosensory perception.
CONCLUSION. The age-related patterns of somatosensory perception from this study refine decision making about performance.
Dédales, carrefours et coïncidences : Paris et la mémoire dans Pont de l’Alma de Julián Ríos
Authors: Shine Laura 
Published: April 2015

La ville peut-elle préserver la mémoire de ceux qui ne sont plus ? Voilà l’une des questions que pose Julián Ríos dans son roman Pont de l’Alma (2010). L’auteur espagnol tisse autour de la princesse Diana un récit de culte et de souvenir, faisant de sa mort dans la Ville Lumière le noeud de son oeuvre complexe. Promenant le lecteur dans un Paris fragmenté et peuplé de revenants, il propose de réexaminer la ville et de remotiver les signes qu’elle offre en les considérant comme des formes d’existence post-mortem. Ce faisant, il donne « sens » au paysage urbain, conjuguant les trois acceptions du mot : sensation, direction et signification.
L’apologue synoptique du « Fort ligoté » (Mt 12,29 et par.) dans la théologie d’Irénée et la première littérature chrétienne
Authors: Bastit Agnès 
Published: April 2015

La présente contribution s’intéresse au micro-récit évangélique transmis par les Synoptiques (Mt 12,29 et par.) et l’Évangile selon Thomas, qui met en scène un conflit pour la soustraction de « biens » à un « Fort » qui les détient (injustement, selon la suggestion implicite du contexte). L’étude s’adonne dans un premier temps à une analyse des diverses formes textuelles sous lesquelles cet apologue a initialement circulé, puis à une enquête sur les diverses lectures et utilisations théologiques du thème dans l’oeuvre d’Irénée à la fin du iie siècle et, au-delà, de quelques-uns de ses contemporains ou continuateurs. Du point de vue du panorama de la présence du verset et de l’histoire de son interprétation dans l’Église ancienne, la recherche a tendu à être exhaustive en ce qui concerne les trois premiers siècles, mais reste indicative pour les quatrième et cinquième siècles (avec une ouverture sur le Moyen Âge pour l’Occident médiéval). La diffusion du récit dans l’Église ancienne est liée à sa fonction kérygmatique : l’économie de la rédemption de l’homme, injustement détenu par le diable, s’y trouve représentée par une action simple et efficace, reliant la vivification ultime de l’homme à sa formation puis captivité initiales, à travers le moment décisif de la libération de l’homme.
Amphipathic DNA Origami Nanoparticles to Scaffold and Deform Lipid Membrane Vesicles
Authors: Czogalla Aleksander , Kauert Dominik J. , Franquelim Henri G. , Uzunova Veselina , Zhang Yixin , Seidel Ralf , Schwille Petra 
Published: April 2015

AbstractWe report a synthetic biology‐inspired approach for the engineering of amphipathic DNA origami structures as membrane‐scaffolding tools. The structures have a flat membrane‐binding interface decorated with cholesterol‐derived anchors. Sticky oligonucleotide overhangs on their side facets enable lateral interactions leading to the formation of ordered arrays on the membrane. Such a tight and regular arrangement makes our DNA origami capable of deforming free‐standing lipid membranes, mimicking the biological activity of coat‐forming proteins, for example, from the I‐/F‐BAR family.
Thermal conductivity and stability of a three‐phase blend of carbon nanotubes, conductive polymer, and silver nanoparticles incorporated into polycarbonate nanocomposites
Authors: Patole Archana , Ventura Isaac Aguilar , Lubineau Gilles 
Published: April 2015

ABSTRACTMetallic and non‐metallic nanofillers can be used together in the design of polycarbonate (PC) nanocomposites with improved electrical properties. Here, the preparation of three‐phase blend (carbon nanotubes (CNT), silver nanoparticles, and conductive polymer) in a two‐step process before incorporation in the PC is reported. First, ethylene diamine functionalized multiwall carbon nanotubes (MWCNT‐EDA) were decorated with Ag nanoparticles. Next, the Ag‐decorated CNTs were coated with poly(3,4‐ethylenedioxythiophene) polystyrene sulfonate (PEDOT : PSS). Due to the high thermal conductivity instrinsic to both metallic and non‐metallic phases, it is expected that the thermal properties of the resulting nanocomposite would largely differ from those of pristine PC. We thus investigated in detail how this hybrid conductive blend affected properties such as the glass transition temperature, the thermal stability, and the thermal conductivity of the nanocomposite. It was found that this strategy results in improved thermal conductivity and thermal stability of the material. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015, 132, 42281.
Similarity measure for type‐2 fuzzy sets with an application to students' evaluation
Authors: Singh Pushpinder 
Published: April 2015

AbstractIn this paper, we propose a new similarity measure for type‐2 fuzzy sets. Validation has been investigated through the consistency of the proposed similarity measure. For practical use, a new method is proposed for the students’ answer book evaluation using the proposed similarity measure. This method is illustrated by the numerical example. © 2015 Wiley Periodicals, Inc. Comput. Appl. Eng. Educ. Comput Appl Eng Educ 23:694–702, 2015; View this article online atwileyonlinelibrary.com/journal/cae; DOI10.1002/cae.21642
Secondary Metabolites of Plants from the Genus Chloranthus: Chemistry and Biological Activities
Authors: Wang An‐Ran , Song Hong‐Chuan , An Hong‐Mei , Huang Qian , Luo Xie , Dong Jin‐Yan 
Published: April 2015

AbstractChloranthus, a genus of the family Chloranthaceae, which is mainly distributed in eastern and southern Asia, has been used in Chinese folk medicine due to its antitumor, antifungal, and anti‐inflammatory activities. This review compiles the research on isolation, structure elucidation, structural diversity, and bioactivities of Chloranthus secondary metabolites reported between 2007 and 2013. The metabolites listed encompass 82 sesquiterpenoids, 50 dimeric sesquiterpenoids, 15 diterpenoids, one coumarin, and five other compounds. Among them, dimeric sesquiterpenoids, the characteristic components of plants from the genus Chloranthus, have attracted considerable attention due to their complex structures and significant biological features, e.g., antitumor, antibacterial, antifungal, anti‐inflammatory, and hepatoprotective activities, and potent and selective inhibition of the delayed rectifier (IK) K+ current and tyrosinase.
Altered Minor‐Groove Hydrogen Bonds in DNA Block Transcription Elongation by T7 RNA Polymerase
Authors: Tanasova Marina , Goeldi Silvan , Meyer Fabian , Hanawalt Philip C. , Spivak Graciela , Sturla Shana J. 
Published: April 2015

AbstractDNA transcription depends upon the highly efficient and selective function of RNA polymerases (RNAPs). Modifications in the template DNA can impact the progression of RNA synthesis, and a number of DNA adducts, as well as abasic sites, arrest or stall transcription. Nonetheless, data are needed to understand why certain modifications to the structure of DNA bases stall RNA polymerases while others are efficiently bypassed. In this study, we evaluate the impact that alterations in dNTP/rNTP base‐pair geometry have on transcription. T7 RNA polymerase was used to study transcription over modified purines and pyrimidines with altered H‐bonding capacities. The results suggest that introducing wobble base‐pairs into the DNA:RNA heteroduplex interferes with transcriptional elongation and stalls RNA polymerase. However, transcriptional stalling is not observed if mismatched base‐pairs do not H‐bond. Together, these studies show that RNAP is able to discriminate mismatches resulting in wobble base‐pairs, and suggest that, in cases of modifications with minor steric impact, DNA:RNA heteroduplex geometry could serve as a controlling factor for initiating transcription‐coupled DNA repair.
Cross‐Coupling of Aryl‐Bromide and Porphyrin‐Bromide on an Au(111) Surface
Authors: Kuang Guowen , Zhang Qiushi , Li Deng Yuan , Shang Xue Song , Lin Tao , Liu Pei Nian , Lin Nian 
Published: April 2015

AbstractCross‐coupling is of great importance in organic synthesis. Here it is demonstrated that cross‐coupling of aryl‐bromide and porphyrin‐bromide takes place on a Au(111) surface in vacuo. The products are oligomers consisting of porphyrin moieties linked by p‐phenylene at porphyrin’s meso‐positions. The ratio of the cross‐coupled versus homocoupled bonds can be regulated by the reactant concentrations. Kinetic Monte Carlo simulations were applied to determine the activation barrier. It is expected that this reaction can be employed in other aryl‐bromide precursors for designing alternating co‐polymers incorporating porphyrin and other functional moieties.
Collective Synthesis of Natural Products Sharing the Dihydro‐γ‐Ionone Core
Authors: Castillo Alexis , Silva Lucia , Briones David , Quílez del Moral José F. , Barrero Alejandro F. 
Published: April 2015

AbstractWe decided to follow the so‐called “collective total synthesis” approach to synthesize several structurally different molecules from a common intermediate possessing appropriate stereochemistry and functionalities. As the common precursor for the efficient preparation of these bioactive molecules, we chose (+)‐3,4‐dihydro‐γ‐ionone (1), a natural compound present in minor quantities in ambergris and in the plant Bellardia trixago. Thus, we report herein the enantioselective synthesis of siccanochromene F (2), metachromins U (3) and V (4), and bicyclic squalene derivative 5 as well as the formal syntheses of ambrein (6), phenazinomycin (7), and (–)‐siccanin (8).
Precursor Modified Electrodes: Electrochemical Detection of Captopril
Authors: Lee Patricia T. , Compton Richard G. 
Published: April 2015

AbstractA fast and facile method for captopril determination is reported using an electrode modified to contain the precursor of a species which can selectively and suitably detect the target. Cyclotriveratrylene (CTV) is first incorporated into a carbon paste electrode then the precursor is converted into cyclotricatechylene (CTC) using voltammetric methods at the point in time when the captopril detection is sought. The resulting cyclotricatechylene (CTC) was used to mediate the electrochemical detection of the thiol containing drug, captopril, via electrocatalytic oxidation. Optimization towards the detection method was investigated by changing experimental conditions including pH and mediator loading. Optimal analytical parameters were determined in acidic conditions which gave a real measurable limit of detection of 1 µM for captopril detection.