bluuebunny/crossref_metadata_2025_split huggingface dataset:
ICAM-5 affects spine maturation by regulation of NMDA receptor binding to α-actinin
Authors: Ning Lin , Paetau Sonja , Nyman-Huttunen Henrietta , Tian Li , Gahmberg Carl G. 
Published: January 2015

ABSTRACTICAM-5 is a negative regulator of dendritic spine maturation and facilitates the formation of filopodia. Its absence results in improved memory functions, but the mechanisms have remained poorly understood. Activation of NMDA receptors induces ICAM-5 ectodomain cleavage through a matrix metalloproteinase (MMP)-dependent pathway, which promotes spine maturation and synapse formation. Here, we report a novel, ICAM-5-dependent mechanism underlying spine maturation by regulating the dynamics and synaptic distribution of α-actinin. We found that GluN1 and ICAM-5 partially compete for the binding to α-actinin; deletion of the cytoplasmic tail of ICAM-5 or ablation of the gene resulted in increased association of GluN1 with α-actinin, whereas internalization of ICAM-5 peptide perturbed the GluN1/α-actinin interaction. NMDA treatment decreased α-actinin binding to ICAM-5, and increased the binding to GluN1. Proper synaptic distribution of α-actinin requires the ICAM-5 cytoplasmic domain, without which α-actinin tended to accumulate in filopodia, leading to F-actin reorganization. The results indicate that ICAM-5 retards spine maturation by preventing reorganization of the actin cytoskeleton, but NMDA receptor activation is sufficient to relieve the brake and promote the maturation of spines.
The CXCR3-CXCL11 signaling axis mediates macrophage recruitment and dissemination of mycobacterial infection
Authors: Torraca Vincenzo , Cui Chao , Boland Ralf , Bebelman Jan-Paul , van der Sar Astrid M. , Smit Martine J. , Siderius Marco , Spaink Herman P. , Meijer Annemarie H. 
Published: January 2015

Abstract
The recruitment of leukocytes to infectious foci depends strongly on the local release of chemoattractant mediators. The human CXC chemokine receptor 3 (CXCR3) is an important node in the chemokine signaling network and is expressed by multiple leukocyte lineages, including T cells and macrophages. The ligands of this receptor originate from an ancestral CXCL11 gene in early vertebrates. Here, we used the optically accessible zebrafish embryo model to explore the function of the CXCR3-CXCL11 axis in macrophage recruitment and show that disruption of this axis increases the resistance to mycobacterial infection. In a mutant of the zebrafish ortholog of CXCR3 (cxcr3.2), macrophage chemotaxis to bacterial infections was attenuated, although migration to infection-independent stimuli was unaffected. Additionally, attenuation of macrophage recruitment to infection could be mimicked by treatment with NBI74330, a high-affinity antagonist of CXCR3. We identified two infection-inducible CXCL11-like chemokines as the functional ligands of Cxcr3.2, showing that the recombinant proteins exerted a Cxcr3.2-dependent chemoattraction when locally administrated in vivo. During infection of zebrafish embryos with Mycobacterium marinum, a well-established model for tuberculosis, we found that Cxcr3.2 deficiency limited the macrophage-mediated dissemination of mycobacteria. Furthermore, the loss of Cxcr3.2 function attenuated the formation of granulomatous lesions, the typical histopathological features of tuberculosis, and led to a reduction in the total bacterial burden. Prevention of mycobacterial dissemination by targeting the CXCR3 pathway, therefore, might represent a host-directed therapeutic strategy for treatment of tuberculosis. The demonstration of a conserved CXCR3-CXCL11 signaling axis in zebrafish extends the translational applicability of this model for studying diseases involving the innate immune system.
Erection pattern and section-wise wettability of honeybee glossal hairs in nectar feeding
Authors: Wu Jianing , Zhu Rengao , Yan Shaoze , Yang Yunqiang 
Published: January 2015

ABSTRACT
The honeybee's tongue (glossa) is covered with bushy hairs and resembles a mop or a brush. We examined the dimensions of glossal hairs of the Italian honeybee (Apis mellifera ligustica) and found that the average length of hairs increased from the proximal segment to the distal end. The glossal dynamic surface of a honeybee in drinking cycles was captured by a specially designed high-speed camera system, and we discovered that the glossal hairs erected rhythmically when drinking nectar; specifically, hairs on the proximal segment erected earlier than those on the distal end of a honeybee's tongue, which was identified as the phenomenon of asynchronous hair erection. Moreover, by measuring the wettability of the tongue, we found that the flabellum was the most hydrophilic and the root of the tongue was hardest to be wetted. According to our observations, we suggest that the honeybee has an optimal hair-erection pattern that could balance nectar intake and viscous drag. These results will be helpful to understand the liquid-feeding mechanism of honeybees, especially the role of erectable glossal hairs.
Vasotocin and isotocin regulate aquaporin 1 function in the sea bream
Authors: Martos-Sitcha Juan Antonio , Campinho Marco Antonio , Mancera Juan Miguel , Martínez-Rodríguez Gonzalo , Fuentes Juan 
Published: January 2015

ABSTRACT
Aquaporins (AQPs) are specific transmembrane water channels with an important function in water homeostasis. In terrestrial vertebrates, AQP2 function is regulated by vasopressin (AVP) to accomplish key functions in osmoregulation. The endocrine control of aquaporin function in teleosts remains little studied. Therefore, in this study we investigated the regulatory role of vasotocin (AVTR) and isotocin (ITR) receptors in Aqp1 paralog gene function in the teleost gilthead sea bream (Sparus aurata). The complete coding regions of Aqp1a, Aqp1b, AVTR V1a2-type, AVTR V2-type and ITR from sea bream were isolated. A Xenopus oocyte-swelling assay was used to functionally characterize AQP1 function and regulation by AVT and IT through their cognate receptors. Microinjection of oocytes with Aqp1b mRNA revealed regulation of water transport via PKA (IBMX+forskolin sensitive), whereas Aqp1a mRNA injection had the same effect via PKC signaling (PDBU sensitive). In the absence of expressed receptors, AVT and IT (10−8 mol l−1) were unable to modify AQP1 function. AVT regulated AQP1a and AQP1b function only when the AVTR V2-type was co-expressed. IT regulated AQP1a function, but not AQP1b, only when ITR was present. Considering that Aqp1a and Aqp1b gene expression in the sea bream intestine is highly salinity dependent in vivo, our results in ovo demonstrate a regulatory role for AVT and IT in AQP1 function in the sea bream in the processing of intestinal fluid to achieve osmoregulation.
Mechanical challenges to freshwater residency in sharks and rays
Authors: Gleiss Adrian C. , Potvin Jean , Keleher James J. , Whitty Jeff M. , Morgan David L. , Goldbogen Jeremy A. 
Published: January 2015

ABSTRACT
Major transitions between marine and freshwater habitats are relatively infrequent, primarily as a result of major physiological and ecological challenges. Few species of cartilaginous fish have evolved to occupy freshwater habitats. Current thought suggests that the metabolic physiology of sharks has remained a barrier to the diversification of this taxon in freshwater ecosystems. Here, we demonstrate that the physical properties of water provide an additional constraint for this species-rich group to occupy freshwater systems. Using hydromechanical modeling, we show that occurrence in fresh water results in a two- to three-fold increase in negative buoyancy for sharks and rays. This carries the energetic cost of lift production and results in increased buoyancy-dependent mechanical power requirements for swimming and increased optimal swim speeds. The primary source of buoyancy, the lipid-rich liver, offers only limited compensation for increased negative buoyancy as a result of decreasing water density; maintaining the same submerged weight would involve increasing the liver volume by very large amounts: 3- to 4-fold in scenarios where liver density is also reduced to currently observed minimal levels and 8-fold without any changes in liver density. The first data on body density from two species of elasmobranch occurring in freshwater (the bull shark Carcharhinus leucas, Müller and Henle 1839, and the largetooth sawfish Pristis pristis, Linnaeus 1758) support this hypothesis, showing similar liver sizes as marine forms but lower liver densities, but the greatest negative buoyancies of any elasmobranch studied to date. Our data suggest that the mechanical challenges associated with buoyancy control may have hampered the invasion of freshwater habitats in elasmobranchs, highlighting an additional key factor that may govern the predisposition of marine organisms to successfully establish in freshwater habitats.
Association between obesity and depression in patients with diabetes mellitus type 2; a study protocol
Authors: De la Cruz-Cano Eduardo , Tovilla-Zarate Carlos Alfonso , Reyes-Ramos Emilio , Gonzalez-Castro Thelma Beatriz , Juarez-Castro Isela , López-Narváez Maria Lilia , Fresan Ana 
Published: January 2015

Background: Diabetes mellitus and depression are highly prevalent conditions throughout the world and have significant impact on health outcomes. It has been estimated that diabetes mellitus type 2 affects about 246 million people in the world; nevertheless, incidence varies among countries. There is evidence that depression is associated with a poor metabolic control in patients with type 2 diabetes mellitus that present other health problems (such as hypertension and obesity). The aim of this study protocol is to determine if obesity increases the risk for depression in patient with diabetes type 2.Methods: The analysis will be reported following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA).The studies suitable for inclusion will be assessed by the Newcastle-Ottawa Scale (NOS) to determine their methodological quality. To identify the studies of interest, we will search on PubMed and EBSCO databases. We will use the following keyword combinations: "Diabetes Mellitus type 2 AND obesity AND depression", "depression AND Diabetes Mellitus type 2", "Diabetes Mellitus type 2 AND body mass index cross sectional study", "depression AND obesity cross-sectional study". Causes for exclusion will be publications that studied patients diagnosed with diabetes mellitus type 1; articles that focused on the treatment and complications of diabetes mellitus type 2; publications that have studied other clinical or psychiatric conditions (for instance, seizure disorder or history of schizophrenia, bipolar disorder, psychotic symptoms or dementia).Conclusion: The results of this study will form the basis for a better understanding of the association between obesity and depression in patients with diabetes mellitus type 2, and will allow development of prediction tools and better interventions. It is evident that several modifiable and non-modifiable risk factors play an important role in the pathogenesis of diabetes among population. Currently, evidence for the deleterious effects of diabetes mellitus type 2 are based on cross-sectional or other observational designs. Therefore, this study will have important implications for future research and public health guidance.
Materials Specification Needs for Future Development of Ultra High Performance Concrete
Authors: Allena Srinivas , Newtson Craig M. 
Published: January 2015

Abstract
Over the next several years, new mixtures for ultra high performance concrete (UHPC) products are expected to move from prepackaged mixtures toward the use of locally available materials to make UHPC economically viable for future projects. Because a wider variety of materials will be used in UHPC, it will be necessary to develop or modify material specifications to ensure high quality UHPC mixtures are produced that provide strength and durability properties consistent with designers’ expectations. New or modified specifications will be needed for chemical requirements on the cement and supplementary cementitious materials considered for use in UHPC as well as to guide producers in the selection of aggregates for use in UHPC. This paper discusses these material issues along with issues related to admixture and fiber selection that are important to the future development of UHPC. Suggestions for development or modification of specifications that will ensure selection of high quality materials and potential performance based specifications that may evolve in the future are also provided.
THERAPY OF ENDOCRINE DISEASE: Response and toxicity of small-molecule tyrosine kinase inhibitors in patients with thyroid carcinoma: a systematic review and meta-analysis
Authors: Klein Hesselink E N , Steenvoorden D , Kapiteijn E , Corssmit E P , van der Horst-Schrivers A N A , Lefrandt J D , Links T P , Dekkers O M 
Published: January 2015

ContextMany tyrosine kinase inhibitors (TKIs) have been studied in patients with thyroid carcinoma (TC). However, the effect and toxicity of various TKIs in differentiated TC (DTC) and medullary TC (MTC) patients have not been directly compared. The aim of the present systematic review and meta-analysis was to systematically summarize response and toxicity of TKIs in TC patients.MethodsAll major databases were systematically searched for publications on TKIs in TC. Primary endpoint was objective response; secondary endpoints were clinical benefit, percentage TKI dose reduction/discontinuation, hand–foot syndrome, diarrhea, and nausea/vomiting. Meta-analysis was performed using an exact likelihood approach and a logistic regression. Pooled percentages and 95% CIs were reported.ResultsIn total, 22 publications were included. For DTC patients, gefitinib induced no objective responses. Pooled percentage was highest for pazopanib, 49 (95% CI 33–64)%, and was 17 (95% CI 12–24)% for sorafenib. For MTC, gefitinib and imatinib induced no objective responses, whereas sunitinib induced objective response in 43 (95% CI 14–77)%. For vandetanib and cabozantinib, these numbers were 40 (95% CI 34–46)% and 27 (95% CI 22–32)% respectively. Clinical benefit was found in 53 (95% CI 48–59)% of DTC patients on sorafenib, and in 84 (95% CI 79–88)% and 55 (95% CI 49–61)% of MTC patients on vandetanib and cabozantinib respectively. All TKIs were associated with considerable toxicity.ConclusionThe currently studied TKIs show a modest response, while side effects are not negligible. Therefore, we suggest to solely consider TKIs in TC patients with rapid progressive disease, for whom the benefits of treatment outweigh toxicity.
Thyroid incidentalomas in patients with multiple endocrine neoplasia type 1
Authors: Lodewijk Lutske , Bongers Pim J , Kist Jakob W , Conemans Elfi B , de Laat Joanne M , Pieterman Carla R C , van der Horst-Schrivers Anouk N A , Jorna Ciska , Hermus Ad R , Dekkers Olaf M , de Herder Wouter W , Drent Madeleine L , Bisschop Peter H , Havekes Bas , Rinkes Inne H M Borel , Vriens Menno R , Valk Gerlof D 
Published: January 2015

ObjectiveCurrently, little is known about the prevalence of thyroid tumors in multiple endocrine neoplasia type 1 (MEN1) patients and it is unclear whether tumorigenesis of these thyroid tumors is MEN1-related. The aim of the study was to assess the prevalence of thyroid incidentalomas in MEN1 patients compared with nonMEN1 patients and to verify whether thyroid tumorigenesis is MEN1-related.DesignA cross-sectional study.MethodsThe study included two groups: patients with MEN1 and a matched non-MEN1 control group without known thyroid disease, who underwent an ultrasound of the neck for the localization of parathyroid adenoma. Ninety-five MEN1 patients underwent ultrasound of the neck and were matched on gender and age with non-MEN1 patients. The prevalence of thyroid incidentalomas described in the ultrasound report was scored. Multinodular goiters, solitary nodes, and cysts were scored as incidentalomas. Presence of nuclear menin expression was evaluated by menin immunostaining of the thyroid tumors.ResultsIn the MEN1 group, 43 (45%) patients had a thyroid incidentaloma compared with 48 (51%) in the non-MEN1 group, of which 14 (15%) and 16 (17%), respectively, were solitary nodes. Menin was expressed in the nuclei of all evaluated thyroid tumors.ConclusionsMEN1 patients do not have a higher prevalence of thyroid incidentalomas compared with primary hyperparathyroidism patients without the diagnosis of MEN1. Menin was expressed in the thyroid tumors of MEN1 patients.
Sex-Ratio Meiotic Drive and Y-Linked Resistance in Drosophila affinis
Authors: Unckless Robert L , Larracuente Amanda M , Clark Andrew G 
Published: January 2015

Abstract
Genetic elements that cheat Mendelian segregation by biasing transmission in their favor gain a significant fitness benefit. Several examples of sex-ratio meiotic drive, where one sex chromosome biases its own transmission at the cost of the opposite sex chromosome, exist in animals and plants. While the distorting sex chromosome gains a significant advantage by biasing sex ratio, the autosomes, and especially the opposite sex chromosome, experience strong selection to resist this transmission bias. In most well-studied sex-ratio meiotic drive systems, autosomal and/or Y-linked resistance has been identified. We specifically surveyed for Y-linked resistance to sex-ratio meiotic drive in Drosophila affinis by scoring the sex ratio of offspring sired by males with a driving X and one of several Y chromosomes. Two distinct types of resistance were identified: a restoration to 50/50 sex ratios and a complete reversal of sex ratio to all sons. We confirmed that fathers siring all sons lacked a Y chromosome, consistent with previously published work. Considerable variation in Y-chromosome morphology exists in D. affinis, but we showed that morphology does not appear to be associated with resistance to sex-ratio meiotic drive. We then used two X chromosomes (driving and standard) and three Y chromosomes (susceptible, resistant, and lacking) to examine fertility effects of all possible combinations. We find that both the driving X and resistant and lacking Y have significant fertility defects manifested in microscopic examination of testes and a 48-hr sperm depletion assay. Maintenance of variation in this sex-ratio meiotic drive system, including both the X-linked distorter and the Y-resistant effects, appear to be mediated by a complex interaction between fertility fitness and transmission dynamics.
Tissue distribution of indium after repeated intratracheal instillations of indium‐tin oxide into the lungs of hamsters
Authors: Tanaka Akiyo , Hirata Miyuki , Matsumura Nagisa , Kiyohara Yutaka 
Published: January 2015

Tissue distribution of indium after repeated intratracheal instillations of indium‐tin oxide into the lungs of hamsters: Akiyo Tanaka, et al. Department of Environmental Medicine, Graduate School of Medical Sciences, Kyushu UniversityObjectiveThe aim of this study was to analyze the tissue distribution of indium after intratracheally instilling indium‐tin oxide (ITO) into the lungs of hamsters.MethodsMale Syrian hamsters received an intratracheal dose of 3 mg/kg or 6 mg/kg of ITO particles containing 2.2 mg/kg or 4.5 mg/kg of indium, twice weekly for 8 weeks. In parallel, control hamsters received only an intratracheal dose of distilled water. A subset of hamsters was euthanized periodically throughout the study from 8 up to 78 weeks after the final instillation. The distribution of indium in the lungs, liver, kidneys and spleen, as well as pathological changes in the liver, kidneys, and spleen, was determined.ResultsThe contents of indium in the lungs in the two ITO groups gradually decreased over the 78‐week observation period, with elimination half‐lives of approximately 142 weeks for the 3 mg/kg ITO group and 124 weeks for the 6 mg/kg ITO. The indium concentrations in the liver, kidneys, and spleen gradually increased throughout the observation period. Although foci of the lesions were observed histopathologically in the extrapulmonary organs among the two ITO groups, the control group showed similar lesions.ConclusionsThe results clearly demonstrate that the clearance of indium from the body is extremely slow after intratracheal instillation in hamsters.
Gifted and Nongifted Students’ Perfectionism, School Motivation, Learning Styles and Academic Achievement
Authors: Altun Fatma , Yazici Hikmet 
Published: January 2015

The main aim of this study was to investigate whether perfectionism, school motivation, learning styles and academic achievement predictors could distinguish gifted students from nongifted students. The sample of the study consists of 386 (Female=164, Male=222) gifted and 410 (Female=209, Male=201) nongifted upper primary students. Information Gathering Form, Positive-Negative Perfectionism, School Motivation and Learning Styles Scales were used as data gathering tools. Independent t- test, Pearson’s coefficient and discriminant analysis were used to analyse the data. The classification results show that the model correctly predicts 98.4% of gifted students and 81% of non-gifted students. This was also discussed with in the light of the current literature. Key words: Academic Achievement; Gifted; Learning Style; Perfectionism; School Motivation.
Using ICT to Support Alumni Data Collection in Higher Education / Upotreba IKT s ciljem pružanja potpore prikupljanju podataka o bivšim studentima u visokom obrazovanju
Authors: Mijić Danijel , Janković Dragan 
Published: January 2015

AbstractGetting feedback from external stakeholders, especially alumni and employers, is a challenging task for higher education institutions. It is important for many reasons, such as providing information to policy makers, assessing quality of academic programmes, adapting academic programmes to labour market needs, and marketing purposes. Alumni and employer surveys are usually used as methods for collecting data from stakeholders. Research suggests that major problems in data collection are related to efficiency and effectiveness of data collection process. This paper presents a different method of data collection supported by ICT. A specially designed web portal equipped with advanced alumni tracking and communication features was developed and used for alumni data collection. The web portal was evaluated after a period of real operation by analyzing collected data and conducting an online survey. The evaluation results showed that the web portal could be used as effective, efficient, and useful tool for alumni data collection and communication between alumni and higher education institutions.Key words: academic quality; employment rate; feedback; graduate tracking; web portal---SažetakDobiti povratne informacije iz vanjskih izvora, osobito od diplomiranih studenata i poslodavaca, predstavlja izazov za visokoškolske institucije. Povratne su informacije korisne zbog nekoliko razloga, kao što su informiranje kreatora politike, vrednovanje kvalitete studijskih programa, prilagođavanje studijskih programa potrebama tržišta rada, marketing. Obično se bivši studenti i poslodavci anketiraju radi prikupljanja podataka. Iz istraživanja proizlazi da se glavni problemi u vezi s prikupljanjem podataka odnose na učinkovitost i djelotvornost procesa prikupljanja. Ovaj rad predstavlja drugačiju metodu prikupljanja podataka uz pomoć IKT jer je u njemu korišten posebno programiran web portal s mogućnostima naprednog praćenja bivših studenata i komunikacije. Web portal je vrednovan nakon određenog razdoblja primjene tako što su analizirani prikupljeni podaci i provedena internetska anketa. Rezultati vrednovanja pokazali su da se web portal može koristiti kao učinkovit i koristan alat za prikupljanje podataka o bivšim studentima i komunikaciju između njih i visokoškolskih institucija. Ključne riječi: kvaliteta nastave; stopa zapošljavanja; povratna informacija; prikupljanje podataka o bivšim studentima; web-portal.