bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduction of eEF2 kinase alleviates the learning and memory impairment caused by acrylamide
Authors: Wang Xiao-Li , Zhang Ru-Nan , Pan Yu-Lin , Li Zhi-Ming , Li Hong-Qiu , Lei Ya-Ting , Zhao Fang-Fang , Hao Xiao-Xiao , Ma Wei-Wei , Yu Cui-Ping , Yao Hong-Wei , Wang Xin-Yu , Lv Jun-Jie , Wu Yong-Hui , Wang Sheng-Yuan 
Published: August 2024

Abstract
Background
The impact of acrylamide (ACR) on learning and memory has garnered considerable attention. However, the targets and mechanisms are still unclear.

Results
Elongation factor 2 (eEF2) was significantly upregulated in the results of serum proteomics. Results from in vitro and in vivo experiments indicated a notable upregulation of Eukaryotic elongation factor 2 kinase (eEF2K), the sole kinase responsible for eEF2 phosphorylation, following exposure to ACR (P < 0.05). Subsequent in vitro experiments using eEF2K siRNA and in vivo experiments with eEF2K-knockout mice demonstrated significant improvements in abnormal indicators related to ACR-induced learning and memory deficits (P < 0.05). Proteomic analysis of the hippocampus revealed Lpcat1 as a crucial downstream protein regulated by eEF2K. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses indicated that eEF2K may play a role in the process of ACR-induced learning and memory impairment by affecting ether lipid metabolism.

Conclusions
In summary, eEF2K as a pivotal treatment target in the mechanisms underlying ACR-induced learning and memory impairment, and studies have shown that it provides robust evidence for potential clinical interventions targeting ACR-induced impairments.
The heterogeneous effects of COVID-19 lockdowns on crime across the world
Authors: Trajtenberg N. , Fossati S. , Diaz C. , Nivette A. E. , Aguilar R. , Ahven A. , Andrade L. , Amram S. , Ariel B. , Arosemena Burbano M. J. , Astolfi R. , Baier D. , Bark H.-M. , Beijers J. E. H. , Bergman M. , Borges D. , Breetzke G. , Cano I. , Concha Eastman I. A. , Curtis-Ham S. , Davenport R. , Droppelman C. , Fleitas D. , Gerell M. , Jang K.-H. , Kääriäinen J. , Lappi-Seppälä T. , Lim W.-S. , Loureiro Revilla R. , Mazerolle L. , Mendoza C. , Meško G. , Pereda N. , Peres M. F. , Poblete-Cazenave R. , Rojido E. , Rose S. , de Ribera O. Sanchez , Svensson R. , van der Lippe T. , Veldkamp J. A. M. , Vilalta Perdomo C. J. , Zahnow R. , Eisner M. P. 
Published: August 2024

AbstractThere is a vast literature evaluating the empirical association between stay-at-home policies and crime during the COVID-19 pandemic. However, these academic efforts have primarily focused on the effects within specific cities or regions rather than adopting a cross-national comparative approach. Moreover, this body of literature not only generally lacks causal estimates but also has overlooked possible heterogeneities across different levels of stringency in mobility restrictions. This paper exploits the spatial and temporal variation of government responses to the pandemic in 45 cities across five continents to identify the causal impact of strict lockdown policies on the number of offenses reported to local police. We find that cities that implemented strict lockdowns experienced larger declines in some crime types (robbery, burglary, vehicle theft) but not others (assault, theft, homicide). This decline in crime rates attributed to more stringent policy responses represents only a small proportion of the effects documented in the literature.
Improved accuracy of continuum surface flux models for metal additive manufacturing melt pool simulations
Authors: Much Nils , Schreter-Fleischhacker Magdalena , Munch Peter , Kronbichler Martin , Wall Wolfgang A. , Meier Christoph 
Published: August 2024

AbstractComputational modeling of the melt pool dynamics in laser-based powder bed fusion metal additive manufacturing (PBF-LB/M) promises to shed light on fundamental mechanisms of defect generation. These processes are accompanied by rapid evaporation so that the evaporation-induced recoil pressure and cooling arise as major driving forces for fluid dynamics and temperature evolution. The magnitude of these interface fluxes depends exponentially on the melt pool surface temperature, which, therefore, has to be predicted with high accuracy. The present work utilizes a diffuse interface finite element model based on a continuum surface flux (CSF) description of interface fluxes to study dimensionally reduced thermal two-phase problems representative for PBF-LB/M in a finite element framework. It is demonstrated that the extreme temperature gradients combined with the high ratios of material properties between metal and ambient gas lead to significant errors in the interface temperatures and fluxes when classical CSF approaches, along with typical interface thicknesses and discretizations, are applied. It is expected that this finding is also relevant for other types of diffuse interface PBF-LB/M melt pool models. A novel parameter-scaled CSF approach is proposed, which is constructed to yield a smoother temperature field in the diffuse interface region, significantly increasing the solution accuracy. The interface thickness required to predict the temperature field with a given level of accuracy is less restrictive by at least one order of magnitude for the proposed parameter-scaled approach compared to classical CSF, drastically reducing computational costs. Finally, we showcase the general applicability of the parameter-scaled CSF to a 3D simulation of stationary laser melting of PBF-LB/M considering the fully coupled thermo-hydrodynamic multi-phase problem, including phase change.
Contrasting responses of motile and non-motile Escherichia coli strains in resuscitation against stable ultrafine gold nanosystems
Authors: Thakur Anindita , Maroju Pranay Amruth , Ganesan Ramakrishnan , Ray Dutta Jayati 
Published: August 2024

AbstractGlobal public health confronts a pressing challenge in antimicrobial resistance (AMR), necessitating urgent intervention strategies due to the low success rate of new antibiotic development. Bacterial motility, beyond conventional antibiotic usage, significantly influences resistance evolution and ecological dynamics. Our recent study marks a breakthrough, revealing the unexplored ability of ultrafine gold nanosystems (UGNs) to inhibit bacterial resuscitation using a motile Escherichia coli (E.coli) K12 strain. We aim to deepen our comparative understanding of UGNs’ efficacy and resuscitation propensity against a non-motile E. coli K12 strain to assess the role of motility. Through UGN application, we identified heritable resistance in both strains, with motile strains exhibiting notably higher mutation rates. Resuscitation experiments unveiled faster recovery in motile strains, attributable to virulence factors, compared to non-motile strains. Additionally, our investigation into aggregation dynamics highlighted the role of protein-mediated aggregation in resistance development to nano-antimicrobials. Overall, the study reveals that the non-motile strains are more susceptible against UGNs, which shows promise in combating AMR.
Optimization of structural reinforcement assessment for architectural heritage digital twins based on LiDAR and multi-source remote sensing
Authors: Shi Yanru , Guo Ming , Zhao Jiawei , Liang Xuanshuo , Shang Xiaoke , Huang Ming , Guo Shuai , Zhao Youshan 
Published: August 2024

Abstract
This study investigates the geometric modelling of architectural heritage digital twins constructed based on multi-source point cloud data and its effectiveness in structural reinforcement assessment. Particular emphasis has been placed on the use of static stiffness rules to identify areas of structural weakness in the geometric models of digital twins and the need for their reinforcement, in order to prevent potential structural problems and to ensure the long-term preservation of the built heritage. Taking Yingxian wooden pagoda as a study case, based on the collection of multi-source point cloud data, the digital twin geometric model is constructed through fine modelling, decoupling of digital models, and geometric transformation. This enhances the true reflection of the column-architrave structure morphology, providing a more accurate model for structural stress analysis. Based on verifying the accuracy of the digital twin geometric model, the instability conditions are identified through static stiffness rules and the deformation values at multiple points are analyzed, enabling precise identification of weak areas in the column-architrave structure. Two types of reinforcement measures are designed and simulated for the structural weak areas identified through the geometric modelling, and the optimal reinforcement scheme is obtained after detailed analysis, according to which specific adjustments and optimization strategies are proposed to enhance the overall stability and durability of the structure. The results showed that the maximum deformation value of 4.65 mm existed in column M2W23, which required reinforcement. Aluminum reinforcement reduced the deformation to 3.5 mm (24.7% reduction), while CFRP fabric reinforcement was more effective, reducing the deformation to 2.8 mm (39.7% reduction), showing high stability. The research results demonstrate the potential application of digital twin technology in architectural heritage preservation and restoration, providing methodological and empirical guidance for heritage preservation research.
Aging time improves adhesive performance of handmade starch paste for restoration of ancient Chinese books and its mechanism of action
Authors: Wang Changwei , Yao Yuqi , Zhang Yue , Yao Xiao 
Published: August 2024

Abstract
In the restoration of ancient Chinese books, handmade starch paste serves as a paper adhesive, distinguished from traditional starch paste preparation methods. It involves special processes such as starch washing and aging, relying entirely on the artisanal expertise throughout the entire process. The study recreates the process of making handmade starch paste for the restoration of traditional ancient books and investigates the effects of aging time on the apparent viscosity, rheological properties, and adhesive performance of the paste. The results indicate that during aging, the pH of the starch paste decreases significantly, but it has a minimal impact on its apparent viscosity, rheological properties, and paper softness. However, it notably enhances the adhesive performance, with the optimal results observed after 3 days of aging. This is attributed to the decrease in residual protein content in the starch, as well as the significant improvement in swelling power and solubility of the starch. The results of infrared spectroscopy and XRD testing reveal that there are no significant changes in the molecular and crystalline structures of starch during the aging process. The acidic environment produced by starch fermentation promotes protein hydrolysis, emerging as the primary reason for the improved adhesive performance of the paste.
Firearm injury hospitalizations and handgun purchaser licensing laws: longitudinal evaluation of state-level purchaser licensure requirements on firearm violence, 2000–2016
Authors: Doucette Mitchell L. , Meyerson Nicholas S. , Crifasi Cassandra K. , Wagner Elizabeth , Webster Daniel W. 
Published: August 2024

Abstract
Background
Handgun purchaser licensing (HPL) laws mandate individuals to obtain a license from law enforcement before buying a firearm. Research indicates these laws effectively reduce various forms of fatal firearm violence, including homicides, suicides, and mass shootings. Our study sought to assess the impact of HPL laws on non-fatal firearm violence.

Methods
Utilizing the augmented synthetic control method (ASCM), we estimated the average treatment effect on the treated (ATT) resulting from a full repeal of an HPL law in Missouri (2007), a partial repeal in Michigan (2012), and an adoption on HPL law in Maryland (2013) on firearm injury hospitalizations. We utilized RAND's healthcare cost and utilization project-based dataset from 2000 to 2016 for our outcome variable. We conducted in-time placebo testing and leave-one-out donor pool testing as sensitivity analyses.

Results
Maryland’s adoption was associated with a statistically significant 32.3% reduction in firearm-related injury hospitalization (FIH) rates (ATT = − 0.497, standard error (SE) = 0.123); Missouri’s repeal was associated with a statistically significant 35.7% increase in FIH rates (ASCM = 0.456, SE = 0.155); and Michigan’s partial repeal showed no statistically significant associations with FIH rates (ATT = − 0.074, SE = 0.129). Sensitivity analyses confirm the robustness of the estimated HPL effects.

Discussion
HPL laws appear to be protective against hospitalizations for nonfatal firearm injuries. These findings align with prior research indicating that HPL laws are effective in reducing fatal firearm violence. States without such licensing systems ought to consider these robust policies as a means to address firearm violence.
Selective disruption of synaptic NMDA receptors of the hippocampal trisynaptic circuit in Aβ pathology
Authors: Alfaro-Ruiz Rocio , Martín-Belmonte Alejandro , Aguado Carolina , Moreno-Martínez Ana Esther , Fukazawa Yugo , Luján Rafael 
Published: August 2024

AbstractSynaptic dysfunction is an early feature in Alzheimer’s disease (AD) pathogenesis and a major morphological correlate of memory deficits. Given the main synaptic location of N-methyl-D-aspartate receptors (NMDARs), their dysregulation has been implicated in these pathological effects. Here, to detect possible alterations in the expression and synaptic localisation of the GluN1 subunit in the brain of amyloidogenic APP/PS1 mice, we employed histoblot and SDS-digested freeze-fracture replica labelling (SDS-FRL) techniques. Histoblots showed that GluN1 expression was significantly reduced in the hippocampus in a layer-dependent manner, in the cortex and the caudate putamen of APP/PS1 transgenic mice at 12 months of age but was unaltered at 1 and 6 months. Using quantitative SDS-FRL, we unravelled the molecular organisation of GluN1 in seven excitatory synapse populations at a high spatial resolution in the CA1 and CA3 fields and the DG of the hippocampus in 12-month-old APP/PS1 mice. In the CA1 field, the labelling density for GluN1 in the excitatory synapses established on spines and interneurons, was significantly reduced in APP/PS1 mice compared to age-matched wild-type mice in the stratum lacunosum-moleculare but unaltered in the stratum radiatum. In the CA3 field, synaptic GluN1 was reduced in mossy fibre-CA3 pyramidal cell synapses but unaltered in the A/C-CA3 pyramidal cell synapses. In the DG, the density of GluN1 in granule cell-perforant pathway synapses was reduced in APP/PS1 mice. Altogether, our findings provide evidence of specific alterations of synaptic GluN1 in the trisynaptic circuit of the hippocampus in Aβ pathology. This differential vulnerability in the disruption of NMDARs may be involved in the mechanisms causing abnormal network activity of the hippocampal circuit and cognitive impairment characteristic of APP/PS1 mice.
Effect of blood pressure control on the risk of proteinuria during bevacizumab treatment in patients with colorectal cancer: a single-center retrospective cohort study
Authors: Nihei Satoru , Asaka Junichi , Yaegashi Mizunori , Asahi Koichi , Kudo Kenzo 
Published: August 2024

Abstract
Purpose
Pre-existing hypertension is reportedly a major risk factor for bevacizumab-induced proteinuria. However, few studies have focused on the effects of blood pressure (BP) control on proteinuria during bevacizumab treatment. We report a retrospective study of the association between poor BP control and the risk of developing proteinuria in patients with colorectal cancer (CRC).

Methods
Data for CRC patients who received bevacizumab between April 2015 and March 2022 were retrospectively collected. Patients were categorized into two groups based on average systolic blood pressure (SBP) during treatment: normal SBP (< 140 mmHg) and high SBP (≥ 140 mmHg). To evaluate the association between average SBP and grade ≥ 2 proteinuria, we used a 3 month landmark analysis and a Cox regression model.

Results
Of the 279 patients analyzed, 109 had high SBP and 170 had normal SBP. The cumulative incidence of grade ≥ 2 and severe proteinuria was significantly higher in the high compared to the normal SBP group (p < 0.001 and p = 0.028, respectively). Landmark analysis indicated significant differences in proteinuria between patients with and without high average SBP during the first 3 months of treatment (p = 0.002 and p = 0.015, respectively). Multivariate analysis showed that average SBP ≥ 140 mmHg was a significant independent risk factor for proteinuria (p = 0.008).

Conclusion
Landmark analysis showed that BP status during the first 3 months of bevacizumab treatment influences the risk of subsequent proteinuria. Therefore, timely diagnosis and stricter BP control are recommended for at least the first 3 months to avoid severe proteinuria.
A case of BIA-ALCL in which postoperative chest wall recurrence was highly suspected: the third reported case of BIA-ALCL in Japan
Authors: Tajiri Wakako , Shimamoto Ryo , Koga Yutaka , Kawasaki Junji , Higuchi Makiko , Nakamura Yoshiaki , Koi Yumiko , Koga Chinami , Ijichi Hideki , Choi Ilseung , Suehiro Youko , Taguchi Kenichi , Tokunaga Eriko 
Published: August 2024

Abstract
Background
Breast implant-associated anaplastic large-cell lymphoma (BIA-ALCL) is a rare malignancy. Many cases of BIA-ALCL are identified based on the presence of late-onset effusion and/or masses. Importantly, the United States Food and Drug Administration noted that in all cases diagnosed in patients with textured implants, the patients either had a history of mixed implantation of smooth and textured devices or no clinical history was supplied for review. In Japan, the first case of BIA-ALCL was reported in 2019, and we encountered the third case in Japan in December 2021. There have been a total of five cases of BIA-ALCL previously reported at Japanese academic conferences (Japan Oncoplastic Breast Surgery Society. http://jopbs.umin.jp/medical/index.html), of which only the first case has been published. Unlike the first case, this patient had clinical features that were highly suggestive of the postoperative chest wall recurrence of breast cancer, with a mass and rash on the skin.

Case presentation
The patient was a 45-year-old woman who had undergone breast reconstruction after breast cancer surgery of the right breast 8 years previously. The patient presented with a mass and skin rash inside the inframammary area, and we suspected a damaged silicone breast implant (SBI) or chest wall recurrence. We examined the mass by a core needle biopsy and made a pathological diagnosis of BIA-ALCL. Imaging findings suggested internal thoracic lymph node swelling and lymphoma infiltration beyond the capsule but no metastatic lesions (cStage III). After en bloc resection of the SBI and lymphoma, adjuvant systemic therapy was performed.

Conclusion
We encountered the third case of BIA-ALCL in Japan. This was a case with clinically advanced stage of disease; however, the BIA-ALCL was found to be in remission.
Failed primary repair of blunt duodenal injury managed by tube duodenostomy, gastrojejunostomy and a feeding jejunostomy: a case report
Authors: Ntongwetape Ngwane , Weledji Elroy Patrick , Mokake Divine Martin Ngomba 
Published: August 2024

Abstract
Background
The worldwide increase in road traffic crashes and use of firearms has increased the incidence of duodenal injuries. Upper gastrointestinal radiological studies and computed tomography (CT) in resource settings may lead to the diagnosis of blunt duodenal injury. Exploratory laparotomy remains the ultimate diagnostic test if a high suspicion of duodenal injury continues in the face of absent or equivocal radiographic signs. Although the majority of duodenal injuries may be managed by simple repair, high-risk duodenal injuries are followed by a high incidence of suture line dehiscence and should be treated by duodenal diversion.

Case presentation
We report a case of a failed primary repair of a blunt injury to the second part of the duodenum (D2) in a 24-year-old African man. This was successfully managed by a tube duodenostomy, a bypass gastrojejunostomy and a feeding jejunostomy in a low resource setting.

Conclusions
Detailed knowledge of the available operative choices in duodenal injury and their correct application is important. When duodenal repair is needed, conservative repair techniques over complex reconstructions should be utilised. The technique of tube duodenostomy can be successfully applied to cases of large defects in the second part of the duodenum (D2), failed previous repair attempts and with defects caused by different aetiology. It may remain especially useful as a damage-control procedure in patients with multiple injuries, significant comorbidities and/or haemodynamic instability.
Can immunocrit be used as a monitoring tool for swine vaccination and infection studies?
Authors: Sagrera Mònica , Sibila Marina , Martínez-Boixaderas Núria , Llorens Anna Maria , Espigares David , Pastor Josep , Garza-Moreno Laura , Segalés Joaquim 
Published: August 2024

Abstract
Background
The immunocrit is a cost-effective and straightforward technique traditionally used to assess passive immunity transfer to newborn piglets. However, it has not been previously used for monitoring the effect of vaccination and/or infections. Therefore, this study aimed to evaluate the usefulness of the immunocrit technique as an immunological monitoring tool in a vaccination and challenge scenario, using porcine circovirus 2 (PCV-2) as pathogen model. The immunocrit ratio was monitored in PCV-2 vaccinated (V) and non-vaccinated (NV) 3-week-old piglets (study day 0, SD0) that were subsequently challenged with this virus at SD21 and followed up to SD42. Additional techniques (PCV-2 IgG ELISA, optical refractometry, and proteinogram) were performed to further characterize the results of the immunocrit analysis.

Results
Immunocrit, γ-globulin concentration and PCV-2 S/P values followed similar dynamics: descending after PCV-2 vaccination but ascending after an experimental PCV-2 inoculation. However, statistically significant differences between V and NV animals were only found with the PCV-2 ELISA. In this case, V animals had significantly higher (p < 0.05) S/P values (S/P ratio = 0.74) than NV (S/P ratio = 0.39) pigs only after challenge at SD42. On the other hand, serum total protein obtained by refractometer (STPr) were maintained from SD0 to SD21 and increased in both groups from SD21 to SD42. Correlations between techniques were low to moderate, being the most robust ones found between immunocrit and optical refractometry (ρ = 0.41) and immunocrit with γ-globulins (ρ = 0.39). In a subset of sera, the proteinogram technique was applied to the whole serum and the supernatant of the immunocrit, with the objective to characterize indirectly the immunocrit fraction. The latter one included all protein types detectable through the proteinogram, with percentages varying between 64.3% (γ-globulins) and 82% (β-globulins).

Conclusion
The immunocrit technique represented a fraction of the total serum proteins, with low to moderate correlation with all the complementary techniques measured in this study. Its determination at different time points did not allow monitoring the effect of vaccination and/or infection using PCV-2 as a pathogen model.