bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-based discovery of pachysiphine synthases inTabernaemontana elegans
Authors: Lezin Enzo , Durand Mickael , Birer Williams Caroline , Lopez Vazquez Ana Luisa , Perrot Thomas , Gautron Nicolas , Pétrignet Julien , Cuello Clément , Jansen Hans J. , Magot Florent , Szwarc Sarah , Le Pogam Pierre , Beniddir Mehdi A. , Koudounas Konstantinos , Oudin Audrey , St-Pierre Benoit , Giglioli-Guivarc’h Nathalie , Sun Chao , Papon Nicolas , Krogh Jensen Michael , Dirks Ron P. , O’Connor Sarah E. , Besseau Sébastien , Courdavault Vincent 
Published: July 2024

AbstractPlant specialized metabolism represents an inexhaustible source of active molecules, some of which have been used in human health for decades. Among these, monoterpene indole alkaloids (MIAs) include a wide range of valuable compounds with anticancer, antihypertensive, or neuroactive properties. This is particularly the case for the pachysiphine derivatives which show interesting antitumor and anti-alzheimer activities but accumulate at very low levels in severalTabernaemontanaspecies. Unfortunately, genome data inTabernaemontanaceaeare lacking and knowledge on the biogenesis of pachysiphine-related MIAsin plantaremains scarce, limiting the prospects for biotechnological supply of many pachysiphine-derived biopharmaceuticals. Here, we report a raw version of the toad tree (Tabernaemontana elegans) genome sequence. These new genomic resources led to the identification and characterization of a couple of genes encoding cytochrome P450 with pachysiphine synthase activity. Our phylogenomic and docking analyses highlights the different evolutionary processes that have been recruited to epoxidize the pachysiphine precursor tabersonine at a specific position and in a dedicated orientation, thus enriching our understanding of the diversification and speciation of the MIA metabolism in plants. These gene discoveries also allowed us to engineer the synthesis of MIAs in yeast through the combinatorial association of metabolic enzymes resulting in the tailor-made synthesis of non-natural MIAs. Overall, this work represents a step forward for the future supply of pachysiphine-derived drugs by microbial cell factories.Significance StatementWhile pachysiphine is a monoterpene indole alkaloid of high interest and the precursor of an anti-Alzheimer compound, its biosynthesis involving the epoxidation of tabersonine remains uncharacterized. By sequencing and assembling the genome ofTabernaemontana elegans, we identified two P450s exhibiting a pachysiphine synthase activity that we modelized to explore the evolutionary scenario leading to the acquisition of this expoxidase activity; and used to engineer yeast cell factories for securing pachysiphine supply and producing new-to-nature alkaloids.
Discovery of chemical marker formaidong(roots ofOphiopogon japonicusandLiriope spicata): a feature-based molecular networking approach
Authors: Lei F.Y. , Saldanha L.L. , Weckerle C. , Bigler L. 
Published: July 2024

AbstractBackgroundDried tuberous roots ofOphiopogon japonicusandLiriope spicataare collectively used asmaidongmedicine in China for the same clinical efficacy-nourishyinand generate fluids, moisten lung and clear heart fire. Extensive cultivation of these species has necessitated the need for stringent quality control measures. To guide quality control efforts effectively, a comprehensive understanding of metabolomic profiles ofmaidongis essential.MethodsMetabolomic profiling was conducted using ultra-high performance liquid chromatography coupled to a timsTOF Pro hybrid quadrupole-time-of-flight mass spectrometer employing trapped ion mobility spectrometry. Data interpretation was enhanced through feature-based molecular networking (FBMN), uni- and multivariate data analysis (MVDA), andin silicoannotation.ResultsThe present study showcases a holistic overview of the metabolomic diversity and variation amongmaidongderived from different origins. Steroidal saponins and homoisoflavonoids were recognized as predominant chemical classes.Ophiopogon japonicuspredominantly exhibited a variety of homoisoflavonoids, whereasLiriope spicatawas characterized by a diversity of steroidal saponins. Characteristic metabolites amongmaidongderived from four origins were highlighted. Annotations of 58 metabolites revealed significant inter-species discrimination, with 6 and 36 metabolites critical for regional differentiation inLiriope spicataandOphiopogon japonicus, respectively.ConclusionThe current approach effectively discriminatedmaidongfrom different origins, and facilitated the selection of chemical markers for quality assessment. This approach supports the advancement of quality control strategies for botanical medicines, particularly those derived from multiple origins, ensuring a more rigorous chemical marker selection for botanical medicines.
The effect of norepinephrine on ovarian dysfunction by mediating ferroptosis in mice model
Authors: Hong Hanqing , Xiao Chengqi , Weng lichun , Wang Qian , Lai Dongmei 
Published: July 2024

AbstractStudies shows that stress is associated with ovarian dysfunction. Norepinephrine (NE), a classic stress hormone in the stress response, is less recognized for its role in ovarian function. A NE-treated mouse model is induced by intraperitoneal injection of NE for 4 weeks. Compared with the normal control, we find that NE-treated mice show disturbances in the estrous cycle, decreased levels of anti-Mullerian hormone (AMH) and estradiol (E2), and increased levels of follicle-stimulating hormone (FSH). Additionally, the number of primordial follicles, primary follicles, secondary follicles, and antral follicles decreased, while the number of atretic follicles increased in NE-treated mice, indicating NE-induced ovarian dysfunction. RNA sequencing further reveals that genes associated with ferroptosis are significantly enriched in NE-treated ovarian tissues. Concurrently, the content of reactive oxygen species (ROS), ferrous ion, and malondialdehyde (MDA) increased, while the expression level of glutathione peroxidase 4 (GPX4) decreased.To elucidate the mechanism of NE-induced ferroptosis in ovaries and the potential reversal by Coenzyme Q10 (CoQ10), an antioxidant, we conduct both in vitro and in vivo experiments. In vitro, we observe that the granulosa cell line KGN, when treated with NE, shows decreased cell viability, reduced expression of GPX4, elevated ferrous ion and ROS content, and increased MDA levels. However, these NE-induced changes are rescued by the addition of CoQ10. In the mouse model, we find that NE-treated mice supplemented with CoQ10 increased GPX4 levels and decreased the contents of iron, ROS, and MDA compared with the NE group. Moreover, the differential expression of genes associated with ferroptosis induced by NE is ameliorated by CoQ10 in NE-treated mice. Additionally, CoQ10 improved ovarian function, as evidenced by increased ovarian weight, more regular estrous cycles, and an increase in follicles at various stages of growth in NE-treated mice. In conclusion, NE induces ovarian dysfunction by triggering ferroptosis in ovarian tissues, and CoQ10 represents a promising approach for protecting reproductive function by inhibiting ferroptosis.
Unsupervised learning as a computational principle works in visual learning of natural scenes, but not of artificial stimuli
Authors: Watanabe Takeo , Sasaki Yuka , Ogawa Daiki , Shibata Kazuhisa 
Published: July 2024

AbstractThe question of whether we learn exposed visual features remains a subject of controversy. A prevalent computational model suggests that visual features frequently exposed to observers in natural environments are likely to be learned. However, this unsupervised learning model appears to be contradicted by the significant body of experimental results with human participants that indicates visual perceptual learning (VPL) of visible task-irrelevant features does not occur with frequent exposure. Here, we demonstrate a resolution to this controversy with a new finding: Exposure to a dominant global orientation as task-irrelevant leads to VPL of the orientation, particularly when the orientation is derived from natural scene images, whereas VPL did not occur with artificial images even with matched distributions of local orientations and spatial frequencies to natural scene images. Further investigation revealed that this disparity arises from the presence of higher-order statistics derived from natural scene images—global structures such as correlations between different local orientation and spatial frequency channels. Moreover, behavioral and neuroimaging results indicate that the dominant orientation from these higher-order statistics undergoes less attentional suppression than that from artificial images, which may facilitate VPL. Our results contribute to resolving the controversy by affirming the validity of unsupervised learning models for natural scenes but not for artificial stimuli. They challenge the assumption that VPL occurring in everyday life can be predicted by laws governing VPL for conventionally used artificial stimuli.
Silent white light: Reduction of the second-order intensity correlation coefficient
Authors: Hansmann Kai Niklas , Dommermuth Franziska , Elsäßer Wolfgang , Walser Reinhold 
Published: July 2024

We investigate the intrawaveguide statistics manipulation of broadband light by combining semiconductor quantum dot physics with quantum optics. By cooling a quantum dot superluminescent diode to the liquid nitrogen temperature of 77 K, Blazek [] have demonstrated a temperature-dependent reduction of the second-order intensity correlation coefficient from 2 for thermal amplified spontaneous emission light to g(2)(0,T=190K)≈1.33. Here, we model the broadband photon statistics assuming amplified spontaneous emission radiation in a pumped, saturable quantum dot gain medium. We demonstrate that, by an intensity increase due to the quantum dot occupation dynamics via the temperature-tuned quasi-Fermi levels, together with the saturation nonlinearity, a statistics manipulation from thermal Bose-Einstein statistics towards Poissonian statistics can be realized, thus producing “silent white light” with a reduced second-order correlation coefficient. Such intensity-noise-reduced broadband radiation is relevant for many applications such as optical coherence tomography, optical communication, or optical tweezers.

Published by the American Physical Society
2024
De-emulsifying barriers to the circular economy-a fertilizer industry perspective
Authors: Katiyar Abhinav , Gedam Vidyadhar V. 
Published: July 2024

PurposeThe fertilizer industry (FI) is well known for its high energy needs, reliance on limited natural resources, and negative environmental impacts (EIs). The consumption of 14.2 billion tons (BT) of materials and the extraction of 1,580 tons of resources per acre are solely attributed to the FI. Because of FI's resource and energy-intensive nature, it becomes crucial for FI to adopt a Circular Economy (CE) to improve efficiency, energy, and resource reuse. However, FI needs to strengthen its progress toward CE adoption. The proposed study comprehends and examines the barriers that inhibit the adoption of CE in FI.Design/methodology/approachA total of 15 barriers obstructing the CE in FI are identified and categorized into seven different categories. The barriers were identified by performing a comprehensive literature review and expert input. The study employs the DEMATEL approach to analyze the barriers and establish a causal relationship between them.FindingsThe study reveals that the most significant challenge to implementing CE in FI is governmental restrictions, which are followed by a lack of awareness and understanding and a need for a steady supply of bulk materials. The results comprehensively comprehend the pivotal factors that jeopardize the CE in FI and furnish a robust foundation for the methodology and tactics to surmount the barriers to CE adoption.Originality/valueThe literature review encompasses the barriers to the transition to CE and offers management and policy perspectives that help the FI's policy and decision-makers surmount these barriers with future research endeavors.
Exploring professional perspectives on integrating generative artificial intelligence into corporate learning and development: an organizational change perspective
Authors: Alhusban Mohammad Issa , Alshurafat Hashem , Khatatbeh Ibrahim N. 
Published: July 2024

Purpose
The primary aim of this study is to investigate the integration of generative artificial intelligence, specifically ChatGPT, into workplace L&D practices, exploring the associated advantages and challenges such integration from an organizational change perspective.

Design/methodology/approach
This study uses a qualitative approach, conducting semi-structured interviews with twelve learning and development (L&D) experts.

Findings
This study indicates that ChatGPT can positively impact L&D by streamlining processes and potentially enhancing employee performance, engagement and satisfaction. However, to mitigate employee resistance, organizations must clearly communicate the necessity and rationale behind the change, involve employees in the implementation process and address trust issues. Key challenges such as overreliance on ChatGPT, AI skill shortages and technology issues like privacy breaches and misinformation must be managed through strong governance frameworks, including policies, guidelines and regular audits.

Research limitations/implications
The study’s scope is confined to semi-structured interviews with L&D experts, potentially limiting its generalizability. Further research could explore the long-term effects and broader implications of ChatGPT integration in different organizational contexts.

Practical implications
By framing GenAI integration within the context of organizational change, this study offers insights into managing the transition effectively by providing guidance for managers on effectively integrating ChatGPT into L&D practices, emphasizing the importance of mitigating potential negative consequences while maximizing benefits.

Social implications
Integrating ChatGPT into organizational L&D has the potential to reshape how employees acquire new skills and knowledge, potentially influencing organizational culture and dynamics. However, careful consideration is required to ensure that the integration process aligns with ethical and social norms, minimizing adverse impacts.

Originality/value
This research contributes foundational insights into the integration of ChatGPT in corporate L&D by researching and understanding the opinions of corporate professionals. It serves as a starting point for organizations to identify challenges in adopting GenAI.
Causal relationship between project financing and overruns in major dam projects in Africa
Authors: Olatunji Oluwole Alfred , Rotimi James Olabode Bamidele , Rotimi Funmilayo Ebun , Silva Chathurani C.W. 
Published: July 2024

PurposeCost and schedule overruns are rife in dam projects. Normative evidence espouses overruns as though they are inimical to development and prosperity aspirations of stakeholders. This study examines the causal relationship between project financing and overruns.Design/methodology/approachCausative data were extracted from completion reports of 28 major dam projects in Africa. Each of the projects was financed jointly by up to 10 international development lenders. Relationships between causes of overruns and project outcomes were analysed.FindingsAnalyses elicit indicators of remarkable correlations between finance procedures and project outcomes. Lenders’ disposition to risk attenuation was the main debacles to project success. Interests had mounted, whilst release of fund was erratic and ill-timed. Finance objectives and mechanisms were grossly inadequate for projects’ intense bifurcations. Projects had slowed or stalled because lenders’ risks attenuation processes were purposed to favour lenders’ objectives, and not projects’ interests. In addition, findings also show project owners’ own funds and the number of lenders to a single project correlate with overruns.Practical implicationsFindings imply commercial complexities around major projects. They also show transactions are shaped by subtle (mis)trust behaviours in project finance procedures. Thus, scholarly solutions to project performance issues should consider behavioural issues of stakeholding parties more broadly, beyond contractors and project owners. Project finance ecosystems are vulnerable to major actors’ self-interests, opportunism and predatory conducts. Borrowers would manage this by developing and improving their capacity to build resilience and trust. Evidence shows intense borrower nations in Africa have limited capacity and acuity for these.Originality/valueThis study contextualises megaprojects in complexity rather than cost. Its additionality is in how finance steers absolute control of project environment away from project owners and how finance administration triggers risks and overrun.
Air pollution and firm performance: the role of innovation and happiness
Authors: Kalash Ismail 
Published: July 2024

Purpose
The detrimental effects of air pollution on the continuity of corporations attract more and more attention in the economic and financial studies. Prior literature investigates the impact of air pollution on corporate financial performance. This study aims to extend this research area by exploring the role of corporate innovation and happiness as factors that mitigate the adverse effects of air pollution and moderate the relationship between air pollution and financial performance.

Design/methodology/approach
This study uses two-step system generalized method of moments models to analyze the data of 200 firms listed on Istanbul Stock Exchange over the period 2009–2022.

Findings
The results show that firms located in regions with higher air pollution are more likely to invest in innovation. In addition, firms that are more exposed to air pollution and have investments in research and development (R&D) have less ability to improve their financial performance compared to firms that have no investments in R&D. In a similar vein, although R&D has positive effect on financial performance, this effect diminishes in the presence of higher air pollution. The results also show that happiness has no significant moderating effect on the relationship between air pollution and financial performance.

Practical implications
The findings of this study related to the role of corporate innovation in determining the effect of air pollution on financial performance indicate that the costs of investment in R&D weaken the firm’s ability to mitigate the adverse impact of air pollution on financial performance, which provides important signals to policymakers to concentrate more on supporting investment in corporate innovation by providing the necessary facilities for firms to improve their innovative performance and decrease the costs of investment in innovation.

Originality/value
To the author’s knowledge, this research is the first to explore the influence of happiness on the air pollution–financial performance relationship. In addition, this study differs from most prior ones by examining how responding to air pollution through investment in innovation can moderate the association between air pollution and financial performance.
Entrepreneurial and international? The role of entrepreneurial orientation in the Brazilian franchises’ internationalization process
Authors: Dermonde Matheus , Fischer Bruno Brandão , Moraes Gustavo Hermínio Salati Marcondes de 
Published: July 2024

PurposeWe investigate the relationship between Entrepreneurial Orientation (EO) and the internationalization pathways of Brazilian franchises. Our aim is to unravel the patterns of firm-level entrepreneurial characteristics vis-à-vis their corresponding processes of internationalization.Design/methodology/approachWe extracted and curated data from the directories of the Brazilian Franchising Association (ABF). Additionally, we scrutinized the International Intensity, International Complexity and EO degree of 27 Brazilian franchises engaged in international activities. Associations between these dimensions were assessed through fuzzy-set qualitative comparative analysis (fsQCA).FindingsOur findings suggest that franchisees can enhance their international activities by adopting various configurations of EO attributes. This discovery illuminates the intricacies of EO and its association with firms’ operations and performance. Accordingly, we empirically demonstrate that EO is not a monolithic element. Instead, it should be perceived as a multifaceted and dynamic construct.Originality/valueThis study aimed to examine the internationalization process of franchises through the EO lens, a perspective that has not been explored in the existing literature. This unique approach offers novel insights about the internationalization processes of this particular business model. Furthermore, our research delves into the intricate relationship between firm-level EO and the trajectories of firm-level internationalization.
How do recommendations influence patient satisfaction? Evidence from an online health community
Authors: Wang Hao , Liu Shan , Gao Baojun , Aziz Arslan 
Published: July 2024

PurposeThis study aims to explore whether seeking recommendations for doctors from offline word-of-mouth or online reviews influences patient satisfaction after treatment, and how the source of recommendation affects this effect.Design/methodology/approachUsing a unique dataset of more than three million reviews from a popular Chinese online health community, this study used the coarsened exact matching method and built fixed-effect models to conduct empirical analysis.FindingsThe results suggest that selecting doctors according to recommendations can improve patient satisfaction and mitigate their dissatisfaction when encountering service failures. However, online recommendations were found to be less effective than offline sources in improving patient satisfaction.Originality/valueThis study provides important insights into patient satisfaction and doctor-patient relationships by revealing the antecedents of satisfaction and the potential for improving this relationship. It also contributes to the understanding of how recommendations in the healthcare context can improve patient satisfaction and alleviate the negative impact of service failures.