bluuebunny/crossref_metadata_2025_split huggingface dataset:
The analgesic effects of Yu-Xue-Bi tablet (YXB) on mice with inflammatory pain by regulating LXA4-FPR2-TRPA1 pathway
Authors: Liu Ying , Zhang Guoxin , Zhu Chunyan , Yao Xuemin , Wang Wenli , Shen Li , Wang Haiping , Lin Na 
Published: August 2024

Abstract
Background
Oxylipins including lipoxin A4 (LXA4) facilitate the resolution of inflammation and possess analgesic properties by inhibiting macrophage infiltration and transient receptor potential (TRP) protein expression. Yu-Xue-Bi Tablet (YXB) is a traditional Chinese patent medicine used to relieve inflammatory pain. Our previous research has shown that the analgesic effect of YXB is related to inhibiting peripheral inflammation and regulating macrophage infiltration, but the mechanism is not yet clear. The purpose of this study is to explore the mechanisms of YXB on mice models with Complete Freund’s Adjuvant (CFA)-induced inflammatory pain from the perspective at the resolution of inflammation.

Methods
Mechanical allodynia thresholds and heat hypersensitivity were measured using the Von Frey test and the hot plate test respectively. The open field test and the tail suspension test were employed to measure anxiety and depressive behaviors respectively. The expression of CD68+ and the proportion of F4/80+CD11b+ cells were measured by immunofluorescence staining and flow cytometry. The expression of transient receptor potential ankyrin 1(TRPA1) was measured by immunofluorescence staining and western blotting. Oxylipins omics analysis provided quantitative data on oxylipins in the paws, and enzyme linked immunosorbent assay (ELISA) was used to measure the levels of LXA4 there. Immunofluorescence staining was used to perform the expression of Leukotriene A4 hydroxylase (LTA4H) in the paws of mice. The impact of injecting the formyl peptide receptor 2(FPR2) antagonist WRW4 and the TRPA1 agonist AITC into the left paws was observed, focusing on the expression of mechanical allodynia thresholds, the expression of CD68+, TRPA1 in the paws, and Calcitonin gene-related peptide (CGRP) in the L5 spinal dorsal horn.

Results
YXB elevated mechanical allodynia thresholds, alleviated heat hypersensitivity and anxiety and depressive behaviors in CFA mice. It significantly reduced the number of CD68+ and proportion of F4/80+CD11b+ within the paws, thereby decreasing macrophage infiltration. Additionally, it diminished the expression of TRPA1 in the paws and TRPV1 in the DRG, leading to an inhibition of peripheral sensitization. Through quantitative analysis, it was found that YXB could modulate DHA-derived oxylipins and LXA4. ELISA results indicated that YXB elevated the levels of LXA4 and inhibited the expression of LAT4H in the paws. Furthermore, the pro-resolution and analgesic effects of YXB were hindered after administration of the FPR2 antagonist. Compared with the AITC group, YXB showed no significant improvement in anti-inflammatory and analgesic effects.

Conclusions
YXB can regulate the oxylipins of paws in CFA mice to promote the resolution of inflammation. The LXA4-FPR2-TRPA1 pathway is a key mechanism for the resolution of inflammation and analgesic effects.
Astrocytic activation increases blood flow in the adult olfactory bulb
Authors: Ogino Takashi , Agetsuma Masakazu , Sawada Masato , Inada Hiroyuki , Nabekura Junichi , Sawamoto Kazunobu 
Published: August 2024

AbstractActivation of astrocytes after sensory stimulation has been reported to be involved in increased blood flow in the central nervous system. In the present study, using a chemogenetic method to induce astrocyte activation in mice without sensory stimulation, we found that astrocytic activation led to increased blood flow in the olfactory bulb, suggesting that astrocyte activation is sufficient for increasing blood flow in the olfactory bulb. The technique established here will be useful for studying the mechanisms underlying sensory input-dependent blood flow increases.
Mother trees of common ash (Fraxinus excelsior) disperse different sets of mycobiome through their seed wings
Authors: Long Feng , Doonan James M. , Nielsen Lene R. , Kjær Erik D. , Kosawang Chatchai 
Published: August 2024

Abstract
Objective
The endophytic mycobiome is present in all studied plant compartments, including fruits and seeds. Here, we studied the mycobiome of seed wings as they are transferred with seeds in common ash and tested whether the mycobiome differs among trees. To achieve this, we used ITS1-based amplicon sequencing and two genotypes of F. excelsior as a model to compare the mycobiome of mother trees and their wings.

Results
We compared the mycobiome of 57 seed wings to the seed stalks (57) collected from two genotypes of F. excelsior using three ramets of each genotype. Alpha diversity indices (ACE, Fisher and Observed OTUs) suggested a higher richness of the mycobiome associated with the seed wing than the seed stalk within each genotype. However, there were neither significant differences in Shannon diversity between the mycobiomes from the two tissue types nor the two genotypes. PERMANOVA revealed significant differences in the mycobiome composition between tissue types (P < 0.001). It also showed a significant difference between seed wings (P = 0.04), but not between seed stalks of the two genotypes. Our results suggest that Fraxinus excelsior mother trees disperse different sets of mycobiomes with their seed wings, which may be important for germination and seedling establishment—especially in the light of ash dieback.
Can the diameter of nerve fibers be effectively utilized to enhance the accuracy of determining the length of the aganglionic segment compared to intraoperative biopsy in patients with Hirschsprung’s disease?
Authors: Talebi Ali , Kahdouei Mohammadsadegh Talebi , Memar Elmira Hajiesmaeil , Ashjaei Bahar , Raji Hojatollah , Adel Maryam Ghavami 
Published: August 2024

Abstract
Objective
The aim of this study is to investigate the accuracy of utilizing neural fiber trunk diameter in accurately diagnosing the length of the aganglionic segment in patients definitively diagnosed with Hirschsprung’s disease.

Results
In this study, 40 patients (19 males, 21 females; mean age 2.5 ± 2.2646 years) were assessed for Hirschsprung’s disease. Constipation was the main symptom (75%), followed by abdominal issues. All underwent contrast enema and rectal suction biopsy for diagnosis, followed by surgery (predominantly Soave and Swensen techniques). Majority (85%) had rectosigmoid involvement. Neural fiber diameter was measured, with 52.5% ≤40 μm and 47.5% >40 μm. Statistical analysis showed 40% sensitivity(CI:95%) and 47% specificity(CI:95%) with a cutoff of 40.5 μm. Cohen’s kappa index for aganglionic segment size was 0.7.
Polypoid heterotopic gastric mucosa: in terminal ileum causing extensive lower gastrointestinal bleeding without Meckel’s diverticulum: a case report
Authors: Aslanabadi Saeid , Badebarin Davoud , Ghavifekr Nazila Hasanzadeh , Ghasemi Babollah , Shoaran Maryam , Hesari Marjan 
Published: August 2024

Abstract
Background
Heterotopic gastric mucosa (HGM) can be located in various parts of the gastrointestinal tract. As a rare anomaly in the small intestine, it can become complicated by intussusception, obstruction, gastrointestinal bleeding, and even peritonitis, leading to death.

Case presentation
This case report focuses on a 12-year-old Middle Eastern boy who presented with hematochezia and abdominal pain for a couple of days. A tagged Red blood cell (RBC) scan and Technetium scan revealed gastrointestinal bleeding at the lower abdomen, highly suggestive of the diagnosis of Meckel’s diverticulum. Subsequently, exploratory laparotomy revealed contiguous and scattered mucosal lesions with multiple polyps of various sizes in the terminal ileum. Meckel’s diverticulum was absent, and the patient was treated with resection and primary anastomosis. The resected tissue revealed extensive ectopic gastric mucosa and polypoid tissues. The patient recovered uneventfully and was discharged four days after the surgery. The symptoms did not recur within six months after his surgery.

Conclusion
Our case demonstrated that despite the rarity of multiple polypoid gastric heterotopias in the terminal ileum, it should be considered as one of the differential diagnoses of gastrointestinal tract bleeding.
Exploring the genetic variability of the PRNP gene at codons 127, 142, 146, 154, 211, 222, and 240 in goats farmed in the Lombardy Region, Italy
Authors: Ferrari Carlotta , Punturiero Chiara , Milanesi Raffaella , Delledonne Andrea , Bagnato Alessandro , Strillacci Maria G. 
Published: August 2024

AbstractScrapie is a transmissible spongiform encephalopathy affecting sheep and goats. The prion protein-encoding gene (PRNP) plays a crucial role in determining susceptibility and resistance to scrapie. At the European level, surveillance of scrapie is essential to prevent the spread of the disease to livestock. According to the Regulation EU 2020/772 polymorphisms K222, D/S146 could function as resistance alleles in the genetic management of disease prevention. In Italy, a breeding plan for scrapie eradication has not been implemented for goats. However, surveillance plans based on the PRNP genotype have been developed as a preventive measure for scrapie. This research aimed to describe the polymorphisms at 7 positions within the PRNP gene in 956 goats of the Alpine, Saanen and mixed populations farmed in the Lombardy Region in Italy. PRNP polymorphisms were detected using single nucleotide polymorphism markers included in the Neogen GGP Goat 70 k chip. The K222 allele occurred in all populations, with frequencies ranging from 2.1 to 12.7%. No animals carried the S/D146 resistance allele. However, it has been demonstrated that polymorphisms in the other positions analysed could influence resistance or susceptibility to scrapie outbreaks in different ways. Ten potentially distinct haplotypes were found, and the most prevalent of the three populations was H2, which differed from the wild type (H1) in terms of mutation (S vs P) at codon 240. This study provided additional information on the genetic variability of the PRNP gene in these populations in the Lombardy region of Italy, contributing to the development of genetic control measures for disease prevention.
Building the fracture network model for the Okuaizu geothermal field based on microseismic data analysis
Authors: Darisma Dian , Mukuhira Yusuke , Okamoto Kyosuke , Aoyogi Naoki , Uchide Takahiko , Ishibashi Takuya , Asanuma Hiroshi , Ito Takatoshi 
Published: August 2024

AbstractUnderstanding flow behavior in a geothermal reservoir is important for managing sustainable geothermal energy extraction. Fluid flow in geothermal reservoirs generally occurs in complex existing fracture systems in which the reservoirs are situated in highly fractured rocks. To simulate a discrete fracture model, the location and orientation of the fracture were computed using statistical processes and observational data. In many cases, estimating the location and orientation of fractures from 1D borehole logging data is challenging. In this study, we used microseismic data to build the fracture network systems and extract the detailed positions and dimensions of the fractures. We used the microseismic data recorded at the Okuaizu Geothermal Field, Fukushima Prefecture, Japan, from 2019 to 2021. First, we located the hypocenters, removing the effect of uncertainty in the velocity structure of the geothermal fluids. We relocated and clustered the seismic events based on waveform similarity. We analyzed each cluster to define the fracture orientation using principal component analysis (PCA) and focal mechanism (FM) analysis. We used the P polarity with the S/P ratio as a constraint for a better fault-plane solution. With PCA, we can extract the fracture dimension of each cluster. Our cluster analysis showed that the clusters were not always planar fractures, and we interpreted them as fracture zones. Based on the consistency between PCA and FM, each cluster/fracture zone was classified into three conceptual models to characterize the fracture network system in this field. This model showed variations in the orientation of small fractures within the fracture zone. We characterized the spatial variation in fracture distribution and orientations in the reservoir and demonstrated the fracture network system of this field. The fracture zone near the injection well has a N–S strike, and the dip is above 80°; however, the fracture zone in the northeastern part of the injection well has a NW–SE strike with a dip between 60° and 80°. The fracture network system estimated in this study is crucial for robust reservoir modeling because our model is more realistic, observation-orientated, and includes local anomalies of reservoir properties.
Graphical Abstract
Radiomics nomogram based on CT radiomics features and clinical factors for prediction of Ki-67 expression and prognosis in clear cell renal cell carcinoma: a two-center study
Authors: Li Ben , Zhu Jie , Wang Yanmei , Xu Yuchao , Gao Zhaisong , Shi Hailei , Nie Pei , Zhang Ju , Zhuang Yuan , Wang Zhenguang , Yang Guangjie 
Published: August 2024

Abstract
Objectives
To develop and validate a radiomics nomogram combining radiomics features and clinical factors for preoperative evaluation of Ki-67 expression status and prognostic prediction in clear cell renal cell carcinoma (ccRCC).

Methods
Two medical centers of 185 ccRCC patients were included, and each of them formed a training group (n = 130) and a validation group (n = 55). The independent predictor of Ki-67 expression status was identified by univariate and multivariate regression, and radiomics features were extracted from the preoperative CT images. The maximum relevance minimum redundancy (mRMR) and the least absolute shrinkage and selection operator algorithm (LASSO) were used to identify the radiomics features that were most relevant for high Ki-67 expression. Subsequently, clinical model, radiomics signature (RS), and radiomics nomogram were established. The performance for prediction of Ki-67 expression status was validated using area under curve (AUC), calibration curve, Delong test, decision curve analysis (DCA). Prognostic prediction was assessed by survival curve and concordance index (C-index).

Results
Tumour size was the only independent predictor of Ki-67 expression status. Five radiomics features were finally identified to construct the RS (AUC: training group, 0.821; validation group, 0.799). The radiomics nomogram achieved a higher AUC (training group, 0.841; validation group, 0.814) and clinical net benefit. Besides, the radiomics nomogram provided a highest C-index (training group, 0.841; validation group, 0.820) in predicting prognosis for ccRCC patients.

Conclusions
The radiomics nomogram can accurately predict the Ki-67 expression status and exhibit a great capacity for prognostic prediction in patients with ccRCC and may provide value for tailoring personalized treatment strategies and facilitating comprehensive clinical monitoring for ccRCC patients.
Characteristics of patients with difficult-to-treat rheumatoid arthritis: a descriptive retrospective cohort study
Authors: Qi Wen , Robert Antoine , Singbo Narcisse , Ratelle Lucie , Fortin Paul R. , Bessette Louis , Brown Jacques P. , Michou Laëtitia 
Published: August 2024

Abstract
Background
In 2021, an EULAR task force published a definition of difficult-to-treat rheumatoid arthritis (D2T RA). Our current knowledge of D2T RA with the EULAR definition is based on European and Asian cohorts, and no North American cohort has yet to be published. The aim of this study was to compare D2T RA patients to non-D2T RA who are good responders to advanced therapy, and to describe their evolution in an university health center patient cohort.

Methods
This is a retrospective single centre study of the medical records of all adults with RA on at least one biologic or target synthetic DMARD (b/tsDMARD). D2T RA group was defined according to the EULAR definition of D2T RA. The non-D2T RA group was defined as a b/tsDMARD good responder who had low-disease activity or remission for at least one year on 1 or 2 b/tsDMARD mechanism of action. We compared the patients’ comorbidities, and history of b/tsDMARD use. Descriptive statistics and proportions were calculated. Kaplan-Meier analysis with log-rank test was used to estimate and compare median survival.

Results
Among the 417 patients, 101 (24%) were D2T RA and 316 (76%) were non-D2T RA. D2T RA group was slightly younger (63 ± 9 years versus 65 ± 12 years, p = 0.045), more likely to have concomitant non-inflammatory pain (28% versus 8%, p < 0.0001) and to discontinue at least one b/tsDMARD due to intolerance (39% versus 10%, p < 0.0001). In the D2T RA group, JAK inhibitors were associated with longer drug continuation when used as the third b/tsDMARD. Fewer patients were using corticosteroid at their most recent follow-up in this Canadian cohort compared to others (16% versus from 29 to 74%).

Conclusion
Concomitant non-inflammatory pain was more prevalent in D2T RA patients compared to b/tsDMARD good responder non-D2T RA patients. Steroid-sparing strategies is possible even in D2T RA patients. Future prospective research may compare JAK inhibitors with other mechanisms of action in D2T RA.
Volatilization of benzene on soil surface under different factors: evaluation and modeling
Authors: Wang Qian , Bian Jianmin , Ruan Dongmei 
Published: August 2024

AbstractThe volatilization of volatile organic compounds following a leakage event is a crucial mechanism that influences their migration and transformation in the soil. It is noteworthy that this process is intricately shaped by soil properties and environmental factors, exhibiting highly complex nonlinear relationships. However, there is currently no reliable mathematical model to predict the nonlinear relationship. To address this gap, the study conducted dynamic experiments considering various factors, including particle size, organic matter content, temperature, wind speed and moisture content. The volatilization rate ($$k$$
k
), an important parameter in volatilization kinetics reflecting the speed of volatilization, was calculated by first-order kinetic principle. Finally, an innovative approach was introduced using a Back Propagation Neural Network (BPNN) model for prediction. The findings indicate that wind speed exerts the most significant impact on the volatilization rate of benzene among the examined factors. The application of BPNN demonstrates the model's accuracy in simulating benzene volatilization rates under diverse conditions. The results of K-fold cross-validation alleviate concerns of potential over-prediction, affirming the reliability of the constructed model. This research introduces a novel methodology for predicting volatilization parameters in real-world scenarios.
From a grand hotel to an urban symbol: the Astor Hotel in old and new Tianjin
Authors: Yang Taoyu 
Published: August 2024

Abstract
This article examines the enduring iconicity, multifaceted functions, and changing meanings of the Astor Hotel in Tianjin—one of the first international hotels in modern China—from the mid-19th century to the 21st century. Originally built more than 150 years ago, the Astor Hotel still stands exactly where it did in the former British Concession in Tianjin. This hotel was not only a venue boasting Tianjin’s most expensive accommodations but also a crucial site where politics, technology, economics, social, and cultural changes intersected and developed in its treaty port incarnation. Moreover, the expansion of the Astor Hotel evolved along with the development of Tianjin from a hypercolonial city to a Chinese-run metropolis. Shortly after the Communist Revolution in China, its ownership was overtaken by the Tianjin Municipal Government. Following a sequence of major renovations and commercial relaunches in the 1990s, the Astor Hotel was restored and reconstructed as an emblem of Tianjin’s historical status as an international and cosmopolitan city in China. Both continuity and changes will be emphasised in the discussion of the Astor Hotel’s history, as well as its multiple functions and shifting symbolic significance.
Multi-resolution analysis enables fidelity-ensured deconvolution for fluorescence microscopy
Authors: Hou Yiwei , Wang Wenyi , Fu Yunzhe , Ge Xichuan , Li Meiqi , Xi Peng 
Published: August 2024

AbstractFluorescence microscopic imaging is essentially a convolution process distorted by random noise, limiting critical parameters such as imaging speed, duration, and resolution. Though algorithmic compensation has shown great potential to enhance these pivotal aspects, its fidelity remains questioned. Here we develop a physics-rooted computational resolution extension and denoising method with ensured fidelity. Our approach employs a multi-resolution analysis (MRA) framework to extract the two main characteristics of fluorescence images against noise: across-edge contrast, and along-edge continuity. By constraining the two features in a model-solution framework using framelet and curvelet, we develop MRA deconvolution algorithms, which improve the signal-to-noise ratio (SNR) up to 10 dB higher than spatial derivative based penalties, and can provide up to two-fold fidelity-ensured resolution improvement rather than the artifact-prone Richardson-Lucy inference. We demonstrate our methods can improve the performance of various diffraction-limited and super-resolution microscopies with ensured fidelity, enabling accomplishments of more challenging imaging tasks.
Induced polarization of clay-rich materials — Part 5: Influence of hyperalkalinity in bentonites
Authors: El Alam J. , Revil A. , Dick P. 
Published: August 2024

A total of 54 new core samples are built by mixing smectite-rich MX80-bentonite and Fontainebleau (silica) sand to study the effect of a hyperalkaline pore solution (including the presence of potassium) on the induced polarization properties of these mixtures. Two types of experiments are performed. In the first set of experiments, we perform induced polarization measurements 10 min after the preparation of the core samples (static experiments) for a broad variety of water contents and saturations. The second set of experiments is performed for over 1000 h to study the potential effect of illitization upon the induced polarization properties (time-lapse experiments). Indeed, the presence of potassium in the hyperalkaline pore solution favors the transformation of smectite into illite. Furthermore, we expect cation replacement (sodium replaced by calcium and potassium) in the smectite interfoliar pore network. For the static experiments, the relationships developed in the previous papers of this series regarding the dependence of the in-phase and quadrature conductivities on the water content and saturation remain valid. For the time-lapse experiments, we observe a decrease in the in-phase and quadrature conductivities over time. These trends can be fitted by a decaying exponential function. This behavior is explained by the kinetics of the illitization process and its effect on the cation exchange capacity of the material. Induced polarization appears therefore as a promising technique to monitor the effect of hyperalkaline solutions associated with the leaching of concrete and permeating a bentonite seal in deep geologic disposal facilities that could be potentially used for high-level and long-lived radioactive wastes.