bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health literacy and ethnic disparities in health-related quality of life among rural women: results from a Chinese poor minority area
Authors: Wang Cuili , Li Hui , Li Lingui , Xu Dongjuan , Kane Robert L , Meng Qingyue 
Published: September 2013

Abstract

Background
We examined the relationship between health literacy (HL) and health-related quality of life (HRQoL) as well as relationship differentials by ethnicity among rural women from a Chinese poor minority area.

Methods
We conducted in-person interviews with 913 rural women aged 23 – 57 (57.5% Hui minorities/42.5% Han ethnicity) enrolled in the Ningxia Women Health Project, gathering data on EQ-5D, self-designed HL, socio-demographic characteristics, and chronic diseases. The extent of impairments in the five dimensions of the EQ-5D was used to measure HRQoL. Factor analysis yielded a single HL factor, which was used as a dichotomous variable in multivariate log-binomial regression models that examined the adjusted association of HL with HRQoL.

Results
Nearly half of the women had no formal education. The most prevalent impairments were pain/discomfort and anxiety/depression (42.42% and 32.09%, respectively). The Hui minorities had 1.65 times higher rates of low HL (defined as less than mean of the factor score for HL) and 1.22 and 1.25 times for pain/discomfort and anxiety/depression impairments, respectively. Low HL was associated with poor HRQoL, with a 23% increase in the prevalence of pain/discomfort impairments after adjusting for socio-demographics. This association was significant in the Hui group (PR=1.30, 95% CI=1.06-1.58) but not for the Han group (PR=0.99, 95% CI=0.76-1.30). HL-stratified analysis revealed modification for ethnic disparities in HRQoL; for pain/discomfort impairments, high HL-PR=0.88 (95% CI=0.71-1.08), low HL-PR=1.24 (95% CI = 1.01-1.52); for anxiety/depression impairments, high HL-PR=0.98 (95% CI=0.73-1.32), low HL-PR=1.44 (95% CI = 1.05-1.98).

Conclusions
Low HL is associated with poor HRQoL across the entire sample and the association may be modified by ethnicity. Similarly, ethnic disparities in HRQoL may be modified by HL, larger in low HL group. Health services should address HL in vulnerable minority women to improve their HRQoL.
Rate-dependent Ca2+ signalling underlying the force-frequency response in rat ventricular myocytes: a coupled electromechanical modeling study
Authors: Krishna Abhilash , Valderrábano Miguel , Palade Philip T , Clark John W 
Published: September 2013

Abstract

Background
Rate-dependent effects on the C
a
2+ sub-system in a rat ventricular myocyte are investigated. Here, we employ a deterministic mathematical model describing various C
a
2+ signalling pathways under voltage clamp (VC) conditions, to better understand the important role of calmodulin (CaM) in modulating the key control variables C
a
2+/calmodulin-dependent protein kinase-II (CaMKII), calcineurin (CaN), and cyclic adenosine monophosphate (cAMP) as they affect various intracellular targets. In particular, we study the frequency dependence of the peak force generated by the myofilaments, the force-frequency response (FFR).

Methods
Our cell model incorporates frequency-dependent CaM-mediated spatially heterogenous interaction of CaMKII and CaN with their principal targets (dihydropyridine (DHPR) and ryanodine (RyR) receptors and the SERCA pump). It also accounts for the rate-dependent effects of phospholamban (PLB) on the SERCA pump; the rate-dependent role of cAMP in up-regulation of the L-type C
a
2+ channel (I

C
a,L
); and the enhancement in SERCA pump activity via phosphorylation of PLB.

Results
Our model reproduces positive peak FFR observed in rat ventricular myocytes during voltage-clamp studies both in the presence/absence of cAMP mediated β-adrenergic stimulation. This study provides quantitative insight into the rate-dependence of C
a
2+-induced C
a
2+-release (CICR) by investigating the frequency-dependence of the trigger current (I

C
a,L
) and RyR-release. It also highlights the relative role of the sodium-calcium exchanger (NCX) and the SERCA pump at higher frequencies, as well as the rate-dependence of sarcoplasmic reticulum (SR) C
a
2+ content. A rigorous C
a
2+ balance imposed on our investigation of these C
a
2+ signalling pathways clarifies their individual roles. Here, we present a coupled electromechanical study emphasizing the rate-dependence of isometric force developed and also investigate the temperature-dependence of FFR.

Conclusions
Our model provides mechanistic biophysically based explanations for the rate-dependence of CICR, generating useful and testable hypotheses. Although rat ventricular myocytes exhibit a positive peak FFR in the presence/absence of beta-adrenergic stimulation, they show a characteristic increase in the positive slope in FFR due to the presence of Norepinephrine or Isoproterenol. Our study identifies cAMP-mediated stimulation, and rate-dependent CaMKII-mediated up-regulation of I

C
a,L
as the key mechanisms underlying the aforementioned positive FFR.
The PKR activator, PACT, becomes a PKR inhibitor during HIV-1 replication
Authors: Clerzius Guerline , Shaw Eileen , Daher Aïcha , Burugu Samantha , Gélinas Jean-François , Ear Thornin , Sinck Lucile , Routy Jean-Pierre , Mouland Andrew J , Patel Rekha C , Gatignol Anne 
Published: September 2013

Abstract

Background
HIV-1 translation is modulated by the activation of the interferon (IFN)-inducible Protein Kinase RNA-activated (PKR). PKR phosphorylates its downstream targets, including the alpha subunit of the eukaryotic translation Initiation Factor 2 (eIF2α), which decreases viral replication. The PKR Activator (PACT) is known to activate PKR after a cellular stress. In lymphocytic cell lines, HIV-1 activates PKR only transiently and not when cells replicate the virus at high levels. The regulation of this activation is due to a combination of viral and cellular factors that have been only partially identified.

Results
PKR is transiently induced and activated in peripheral blood mononuclear cells after HIV-1 infection. The addition of IFN reduces viral replication, and induces both the production and phosphorylation of PKR. In lymphocytic Jurkat cells infected by HIV-1, a multiprotein complex around PKR contains the double-stranded RNA binding proteins (dsRBPs), adenosine deaminase acting on RNA (ADAR)1 and PACT. In HEK 293T cells transfected with an HIV-1 molecular clone, PACT unexpectedly inhibited PKR and eIF2α phosphorylation and increased HIV-1 protein expression and virion production in the presence of either endogenous PKR alone or overexpressed PKR. The comparison between different dsRBPs showed that ADAR1, TAR RNA Binding Protein (TRBP) and PACT inhibit PKR and eIF2α phosphorylation in HIV-infected cells, whereas Staufen1 did not. Individual or a combination of short hairpin RNAs against PACT or ADAR1 decreased HIV-1 protein expression. In the astrocytic cell line U251MG, which weakly expresses TRBP, PACT mediated an increased HIV-1 protein expression and a decreased PKR phosphorylation. In these cells, a truncated PACT, which constitutively activates PKR in non-infected cells showed no activity on either PKR or HIV-1 protein expression. Finally, PACT and ADAR1 interact with each other in the absence of RNAs.

Conclusion
In contrast to its previously described activity, PACT contributes to PKR dephosphorylation during HIV-1 replication. This activity is in addition to its heterodimer formation with TRBP and could be due to its binding to ADAR1. HIV-1 has evolved to replicate in cells with high levels of TRBP, to induce the expression of ADAR1 and to change the function of PACT for PKR inhibition and increased replication.
Molecular and phylogenetic analysis of the porcine kobuvirus VP1 region using infected pigs from Sichuan Province, China
Authors: Chen Lei , Zhu Ling , Zhou Yuan-cheng , Xu Zhi-wen , Guo Wan-zhu , Yang Wen-yu 
Published: September 2013

Abstract

Background
Porcine kobuvirus (PKoV) is a member of the Kobuvirus genus within the Picornaviridae family. PKoV is distributed worldwide with high prevalence in clinically healthy pigs and those with diarrhea.

Methods
Fecal and intestinal samples (n = 163) from pig farms in Sichuan Province, China were obtained to determine the presence of PKoV using reverse transcription polymerase chain reaction assays. Specific primers were used for the amplification of the gene encoding the PKoV VP1 protein sequence. Sequence and phylogenetic analyses were conducted to clarify evolutionary relationships with other PKoV strains.

Results
Approximately 53% (87/163) of pigs tested positive for PKoV. PKoV was widespread in asymptomatic pigs and those with diarrhea. A high prevalence of PKoV was observed in pigs younger than 4 weeks and in pigs with diarrhea. Phylogenetic analysis of 36 PKoV VP1 protein sequences showed that Sichuan PKoV strains formed four distinct clusters. Two pigs with diarrhea were found to be co-infected with multiple PKoV strains. Sequence and phylogenetic analyses revealed diversity within the same host and between different hosts. Significant recombination breakpoints were observed between the CHN/SC/31-A1 and CHN/SC/31-A3 strains in the VP1 region, which were isolated from the same sample.

Conclusion
PKoV was endemic in Sichuan Province regardless of whether pigs were healthy or suffering from diarrhea. Based on our statistical analyses, we suggest that PKoV was the likely causative agent of high-mortality diarrhea in China from 2010. For the first time, we provide evidence for the co-existence of multiple PKoV strains in one pig, and possible recombination events in the VP1 region. Our findings provide further insights into the molecular properties of PKoV, along with its epidemiology.
Pathways to psychiatric care in urban north China: a general hospital based study
Authors: Zhang Weijun , Li Xuemei , Lin Yan , Zhang Xiulan , Qu Zhiyong , Wang Xiaohua , Xu Huiwen , Jiao Alvina , Guo Mengqi , Zhang Yurong , Li Yafang , Tian Donghua 
Published: September 2013

AbstractBackgroundPathway studies highlight the help-seeking behaviors of patients with physical and mental illnesses. A number of studies in this field have been completed in various parts of the world. The purpose of this study is to explore the characteristics of the help-seeking pathways of patients with mental illness from urban north China at Mental Health Professional (MHP).MethodsThe pathway diagrams, which accounted for more than five percent of patients, were documented for 441 subjects using the translated version of the World Health Organization (WHO) pathway encounter form. The patterns and durations of care-seeking were analyzed in different diagnostic groups. The χ2-test and the Mann-Whitney U test were employed, as needed.ResultsRespondents visited the MHP through a variety of pathways. Approximately three-quarters of the patients took an indirect pathway (74.8% vs 25.2%, χ2 = 108.8,p < 0.0001), and on average, each patient consulted 3.4 caregivers. The vast majority of patients first visited local tertiary general hospitals (56.4%vs4.1%, χ2 = 138.3,p < 0.0001) or local secondary general hospitals (24.8%vs4.1%, χ2 = 40.96,p < 0.0001). However, only 9.6% of patients were diagnosed with mental disorders for the patients who first visited non-psychiatric hospitals. Of the patients who first contacted with psychiatry hospital, 55.6% received a professional diagnosis and finally reached the MHP because of the poor treatment or high-cost medical care.ConclusionsThe majority of patients seek other pathways than to go to MHP directly and this may be due to stigma, and/or lack of knowledge. The study gives emphasis on the importance of improving skills and knowledge that will facilitate the recognition of psychiatric disorders in the community health centers, the general hospitals system and by private practitioners. The pathway described by this study may be helpful while preparing mental health programs in the future.
Comparative proteomics analysis of normal and memory-deficient Drosophila melanogaster heads
Authors: Lee Tian-Ren , Lee Hsiao-Yun , Huang Shun-Hong , Chan Hsin-Tzu , Lyu Ping-Chiang , Chan Hong-Lin 
Published: September 2013

Abstract

Background
Learning and memory are extremely complex and dynamic processes. Proteins that participate in memory formation are strictly regulated by various pathways and may require protein synthesis and/or post-translational modifications. To examine the formation of memory, Drosophila was genetically engineered with the mutated memory-related gene, Amn

X8
, which induces normal learning and memory behavior within the first 30 min of training. However, the process through which learning occurred could not be retained after the 30 min of training, indicating that these mutants possessed deficits in middle-term memory. A proteomics platform based on two-dimensional differential gel electrophoresis and matrix-assisted laser desorption/ionization time of flight mass spectrometry was employed to examine the head proteome alterations between the wild-type 2u strain and the memory-deficient mutant Amn

X8
strain.

Results
The results indicated that 30 differentially expressed head proteins that mainly function in metabolic pathways and cell structure/cytoskeleton proteins were involved in memory formation. A bioinformatics analysis demonstrated that mitochondrial proteins had critical roles in modulating this process.

Conclusions
This is the first study of a comparative head proteomics analysis of a memory mutant strain and a normal control fruit fly strain. The fundamental proteomics analysis provides potential candidates for further elucidation of the biological mechanism of the memory formation process in Drosophila.
Methotrexate treatment of FraX fibroblasts results in FMR1 transcription but not in detectable FMR1 protein levels
Authors: Brendel Cornelia , Mielke Benjamin , Hillebrand Merle , Gärtner Jutta , Huppke Peter 
Published: September 2013

Abstract

Background
Fragile X syndrome is caused by the loss of FMRP expression due to methylation of the FMR1 promoter. Treatment of fragile X syndrome patients’ lymphoblastoid cells with 5-azadeoxycytidine results in demethylation of the promoter and reactivation of the gene. The aim of the study was to analyze if methotrexate, an agent which also reduces DNA methylation but with less toxicity than 5-azadeoxycytidine, has therapeutic potential in fragile X syndrome.

Methods
Fibroblasts of fragile X syndrome patients were treated with methotrexate in concentrations ranging from 1 to 4 μg/ml for up to 14 days. FMR1 and FMRP expression were analyzed by quantitative PCR and western blotting.

Results

FMR1 mRNA was detected and levels correlated positively with methotrexate concentrations and time of treatment, but western blotting did not show detectable FMRP levels.

Conclusions
We show that it is possible to reactivate FMR1 transcription in fibroblasts of fragile X syndrome patients by treatment with methotrexate. However, we were not able to show FMRP expression, possibly due to the reduced translation efficacy caused by the triplet repeat extension. Unless FMR1 reactivation is more effective in vivo our results indicate that methotrexate has no role in the treatment of fragile X syndrome.
Osteoradionecrosis of the mandible: A case series at a single institution
Authors: Gevorgyan Artur , Wong Kevin , Poon Ian , Blanas Nick , Enepekides Danny J , Higgins Kevin M 
Published: September 2013

Background Osteoradionecrosis (ORN) defines exposed irradiated bone, which fails to heal over a period of 3–6 months without evidence of residual or recurrent tumor. In the previous decades, a staging and treatment protocol suggested by Marx, has dominated the approach to ORN. However, recently this paradigm is shifting. The purpose of this study was to evaluate our institutional experience in managing ORN through a retrospective review of case series from a large urban academic cancer centre. Methods A retrospective chart review was conducted to include all ORN cases from 2003 to 2009 diagnosed at the Department of Otolaryngology – Head and Neck Surgery and the Department of Dentistry. The staging of ORN was assessed as affected by tumor site, tumor stage, radiotherapy modality and dose, chemotherapy, dental work, and time to diagnosis. The effectiveness of hyperbaric oxygen therapy (HBO) and surgery in the management of ORN was evaluated. Results Fourteen cases of ORN were documented (incidence 0.84%). Primary subsites included tonsils, tongue, retromolar trigone, parotid gland, soft palate and buccal mucosa. There were 5 (35.7%) stage 1, 3 (21.4%) stage 2, and 6 (42.9%) stage 3 cases. ORN severity was not significantly associated with gender, smoking, alcohol use, tumor site, T stage, N stage, AJCC stage, or treatment modality (radiation alone, surgery with adjuvant radiation or adjuvant chemoradiation). Patients treated with intensity-modulated radiotherapy developed less severe ORN compared to those treated with conventional radiotherapy (p < 0.015). ORN stage did not correlate with radiation dose. In one patient only dental procedures were performed following radiation and could be implicated as the cause of ORN. HBO therapy failed to prevent ORN progression. Surgical treatment was required for most stage 2 (partial resections and free tissue transfers) and stage 3 patients (mandibulectomies and free tissue transfers, including two flaps in one patient). At an average follow up of 26 months, all patients were cancer-free, and there was no evidence of ORN in 84% of patients. Conclusions In early ORN, we advocate a conservative approach with local care, while reserving radical resections with robust reconstruction with vascularized free tissue for advanced stages.
Does production of oil palm, soybean, or jatropha change biodiversity and ecosystem functions in tropical forests
Authors: Savilaakso Sini , Laumonier Yves , Guariguata Manuel R , Nasi Robert 
Published: September 2013

Abstract
Background
Biofuels, or fuels derived from transformation of biological matter, are hailed by some as a promising source of renewable energy potentially reducing greenhouse gas emissions. A widespread adoption of biofuels will however present its own set of challenges and consequences. Direct or indirect land use change due to expansion of feedstock cultivation can cause deforestation and forest degradation leading to biodiversity losses and other environmental concerns like soil degradation and erosion, water pollution and scarcity, and the risk of crop species invading natural ecosystems.
Although biofuel production is currently not the main use of palm oil and soybean and hence, has so far contributed only little to the land-use change patterns, it has been predicted to grow. Therefore, it is important to know the potential consequences of the expansion of biofuel cultivation may have for biodiversity in order to provide policy guidance.

Methods/design
In this review, we will assess the current state of knowledge of the impact of three first generation biofuel crops - oil palm, soybean, and jatropha - on the biodiversity and ecosystem functions of the tropical forests. We will look at the additional comparison of impacts from industrial versus smallholder plantations, and will compare the mitigation potential of different standards related to biofuel production. We will consider both qualitative and quantitative primary studies as well as descriptive reports that compare land conversion for target crop production with other land uses or land cover types. Both before/after and site comparison studies will be included, and biodiversity indicators to be assessed are species richness, abundance, and plant and animal community composition. If there is enough data, quantitative meta-analysis will be performed. Otherwise results will be summarized narratively.
Investigation of the painting materials in Zhongshan Grottoes(Shaanxi, China)
Authors: Egel Ellen , Simon Stefan 
Published: September 2013

Abstract
This paper reports on the characterization of paint samples from polychrome sculptures in the main cave of the Zhongshan Grottoes, China. Optical Microscopy (OM), Environmental Scanning Electron Microscopy in combination with Energy Dispersive X-ray analysis (ESEM/EDX), Fourier Transform Infrared spectroscopy (FTIR) and Raman spectroscopy were carried out in order to study the stratigraphy of the sculptural polychromy and to determine the painting materials.
Minium Pb3O4 and mercury sulphide HgS, cinnabar or its synthetic form vermilion were found as red pigments. Two mixtures were used to produce a rose color: lead white Pb3 (CO3)2 (OH)2 with minium and hematite added to gypsum. Yellow was attributed to an ochre. The green paint layer has been identified as botallackite [Cu2(OH)3Cl], an isomer of atacamite and paratacamite. Copper oxalate was also found in this green paint layer and calcium oxalate were detected in a numerous of paint layers without restriction to any specific colors.
Pigments and their use as mixture or as overlapping different paint layers in Zhongshan Grottoes were identified on a selected number of samples. Over painted areas could be identified and two reasons could explained the blackening of the paintings: a loss of the fragilized colored paint layer, which make the underneath black paint layer visible and a darkening of the upper paint layer, due to the burning of the candles in the temple.
Furthermore, FTIR analysis performed on the samples give the indication of an oily binder.
Biochemical and kinetic characterisation of a novel xylooligosaccharide-upregulated GH43 β-d-xylosidase/α-l-arabinofuranosidase (BXA43) from the probiotic Bifidobacterium animalis subsp. lactis BB-12
Authors: Viborg Alexander Holm , Sørensen Kim Ib , Gilad Ofir , Steen-Jensen Daniel Bisgaard , Dilokpimol Adiphol , Jacobsen Susanne , Svensson Birte 
Published: September 2013

Abstract
The Bifidobacterium animalis subsp. lactis BB-12 gene BIF_00092, assigned to encode a β-d-xylosidase (BXA43) of glycoside hydrolase family 43 (GH43), was cloned with a C-terminal His-tag and expressed in Escherichia coli. BXA43 was purified to homogeneity from the cell lysate and found to be a dual-specificity exo-hydrolase active on para-nitrophenyl-β-d-xylopyranoside (p NPX), para-nitrophenyl-α-L-arabinofuranoside (p NPA), β-(1 → 4)-xylopyranosyl oligomers (XOS) of degree of polymerisation (DP) 2–4, and birchwood xylan. A phylogenetic tree of the 92 characterised GH43 enzymes displayed five distinct groups (I − V) showing specificity differences. BXA43 belonged to group IV and had an activity ratio for p NPA:p NPX of 1:25. BXA43 was stable below 40°C and at pH 4.0–8.0 and showed maximum activity at pH 5.5 and 50°C. K
m and k
cat for p NPX were 15.6 ± 4.2 mM and 60.6 ± 10.8 s-1, respectively, and substrate inhibition became apparent above 18 mM p NPX. Similar kinetic parameters and catalytic efficiency values were reported for β-d-xylosidase (XynB3) from Geobacillus stearothermophilus T‒6 also belonging to group IV. The activity of BXA43 for xylooligosaccharides increased with the size and was 2.3 and 5.6 fold higher, respectively for xylobiose and xylotetraose compared to p NPX. BXA43 showed clearly metal inhibition for Zn2+ and Ag+, which is different to its close homologues. Multiple sequence alignment and homology modelling indicated that Arg505Tyr506 present in BXA43 are probably important for binding to xylotetraose at subsite +3 and occur only in GH43 from the Bifidobacterium genus.