bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Health Literacy on Drug Adherence in Patients with Heart Failure
Authors: Noureldin Marwa , Plake Kimberly S. , Morrow Daniel G. , Tu Wanzhu , Wu Jingwei , Murray Michael D. 
Published: September 2012

Study ObjectiveTo assess the effect of health literacy on drug adherence in the context of a pharmacist‐based intervention for patients with heart failure.DesignPost hoc analysis of a randomized controlled trial.SettingInner‐city ambulatory care practice affiliated with an academic medical center.PatientsThe original trial enrolled 314 patients with heart failure who were aged 50 years or older and were taking at least one cardiovascular drug for heart failure; 122 patients received the pharmacist intervention (patient education, therapeutic monitoring, and communication with primary care providers), and 192 patients received usual care (regular follow‐up with primary care providers). We analyzed the results of 281 patients who had available health literacy and adherence data.Measurements and Main ResultsDrug adherence was assessed over 9 months using electronic prescription container monitors on cardiovascular drugs. Health literacy was assessed using the Short Test of Functional Health Literacy in Adults (scores range from 0–36, with an adequate literacy score defined as ≥ 23). Taking adherence, defined as the percentage of prescribed drug doses taken by the patient compared with the number of doses prescribed by the physician, was assessed for each group. Patients were a mean ± SD of 63 ± 9 years old, 51% had less than 12 years of education, 29% had inadequate health literacy, and they received a mean ± SD of 11 ± 4 drugs. In the usual care group, taking adherence was greater among patients with adequate (69.4%) than those with inadequate (54.2%) health literacy (p=0.001). In the intervention group, the difference in taking adherence among patients with adequate (77.3%) and inadequate (65.3%) health literacy was not statistically significant (p=0.06). Among patients with inadequate health literacy, the intervention increased adherence (65%, 95% confidence interval [CI] 54–77%) by an order of magnitude similar to that of the baseline adherence of patients with adequate health literacy (69%, 95% CI 65–74%). Multivariable analysis supported the association between health literacy and adherence.ConclusionIn patients with heart failure, those with adequate health literacy have better adherence to cardiovascular drugs than those with inadequate health literacy. The pharmacist intervention improved adherence in patients with adequate and inadequate health literacy. Health literacy may be an important consideration in drug adherence interventions.
Enhanced osteoinductivity and osteoconductivity through hydroxyapatite coating of silk‐based tissue‐engineered ligament scaffold
Authors: He Pengfei , Sahoo Sambit , Ng Kian Siang , Chen Kelei , Toh Siew Lok , Goh James Cho Hong 
Published: September 2012

AbstractHybrid silk scaffolds combining knitted silk fibers and silk sponge have been recently developed for use as ligament‐alone grafts. Incorporating an osteoinductive phase into the ends of a ligament scaffold may potentially generate an integrated “bone–ligament–bone” graft and improve graft osteointegration with host bone. To explore the possible application of hydroxyapatite (HA) coating in the fabrication of osteoinductive ends of silk‐based scaffold, HA was coated on the hybrid silk scaffold and the effects to the bone‐related cells were evaluated. HA could be coated in a uniform and controlled manner on the silk sponge, using an alternate soaking technology, with the amount deposited being dependent on the number of soaking cycles. HA coating also progressively reduced the hydrophobicity of silk surface (decreasing water contact angle from 87° to 42–76°, after 1–3 soaking cycles), making the HA‐coated silk scaffold less favorable for initial cell attachments; but the attached cells showed viability and sustained proliferation on the HA‐coated scaffold. As demonstrated by real‐time polymerase chain reaction and alkaline phosphatase assay, the osteoinductivity of HA‐coated silk scaffolds resulted in the osteogenic differentiation of bone marrow mesenchymal stem cells, and the osteoconductivity of HA‐coated silk scaffolds supported osteoblasts growth and maintained the properties of mature osteoblasts. These properties of HA‐coating demonstrated its possible application in fabricating osteoinductive ends of the silk‐based ligament graft to potentially enhance graft‐to‐host bone integration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
The effect of rotative stress on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts
Authors: Qing Hong Zhang , Meng Tao Liu , Yi Zhou , Wei Liu , Ju Xiang Shen , Li Liu 
Published: September 2012

AbstractThis study was designed to explore the effects of rotative stress on carbonic anhydrase II (CAII), TNF receptor superfamily member 6 (FAS), FAS ligand (FASL), osteoclast‐associated receptor (OSCAR), and tartrate‐resistant acid phosphatase (TRAP) gene expression in osteoclasts. Osteoclasts were induced from RAW264.7 cells cultured in medium containing recombinant murine soluble receptor activator of NF‐Kβ ligand (sRANKL). The mRNA and protein expression of CAII, FAS, FASL, OSCAR, and TRAP genes in osteoclasts was detected by RT‐PCR and Western blot, respectively, after osteoclasts were loaded at various rotative stress strengths and times. No significant differences in mRNA and protein expression were observed between any of the control groups (P > 0.05). Importantly, rotative stress had a significant effect on the mRNA and protein expression of these genes (P < 0.05). We found a negative relationship between rotative stress strength and prolonged loading time and the expression of FAS/FASL genes in osteoclasts. In addition, there was a positive relationship between rotative stress strength and prolonged loading time and the expression of CAII, OSCAR, or TRAP genes in osteoclasts. Based on these results, rotative stress has a significant effect on CAII, FAS, FASL, OSCAR, and TRAP gene expression in osteoclasts. J. Cell. Biochem. 114: 388–397, 2013. © 2012 Wiley Periodicals, Inc.
Celastrol attenuates adipokine resistin‐associated matrix interaction and migration of vascular smooth muscle cells
Authors: Kang Sang‐Wook , Kim Min Soo , Kim Hyun‐Sung , Kim Yunho , Shin Daekeun , Park Jung Han Yoon , Kang Young‐Hee 
Published: September 2012

AbstractObesity instigates various health problems such as atherosclerosis, diabetes, and cancer. Resistin, an adipose tissue‐specific secretory adipokine, operates endocrine functions through increasing insulin resistance. Vascular smooth muscle cells (SMC) migrate into the subendothelial space and proliferate, thereby contributing to neointimal formation in atherosclerosis and restenosis. The aim of this study was to elucidate whether celastrol obtained from Tripterygium wilfordii Hook, inhibited human aortic SMC migration. Celastrol capable of antagonizing inflammatory responses attenuated the resistin secretion from THP‐1‐derived macrophages. The macrophage‐conditioned media promoted SMC proliferation and MMP‐2 production, which was dampened by 10–100 nM celastrol. Celastrol encumbered the SMC migration in response to 50 ng/ml resistin, concomitant with the inhibition of induction of connective tissue growth factor and collagen I/IV. In addition, celastrol disabled human aortic SMC exposed to resistin from migrating. The resistin‐induced shedding of integrin β2/β3 expression was demoted by celastrol, thereby contributing to the inhibition of collagen matrix‐SMC interaction. Next, resistin‐induced Toll‐like receptor‐4 (TLR‐4) expression was abrogated by celastrol, indicating that TLR‐4 was the resistin signaling receptor that was blocked by celastrol. Collectively, these results demonstrate that anti‐inflammatory celastrol blunted the macrophage secretion of the adipokine resistin, and suppressed the SMC migration by disturbing the interaction between SMC and intimal collagen matrix. Therefore, celastrol may inhibit atherogenic migration of vascular SMC upon resistin loading by intimal macrophages within atherosclerotic lesions. J. Cell. Biochem. 114: 398–408, 2013. © 2012 Wiley Periodicals, Inc.
The influence of RAMP1 overexpression on CGRP‐induced osteogenic differentiation in MG‐63 cells in vitro: An experimental study
Authors: Zhao Zhiliang , Fu Xiaobing , Zhang Gang , Li Yan , Wu Meng , Tan Yinghui 
Published: September 2012

AbstractThe aim of this study was to elucidate the influence of receptor activity modifying protein 1 (RAMP1) overexpression on the expression and distribution of calcitonin receptor‐like receptor (CRLR) in MG‐63 cells. Our research also focused on whether RAMP1 overexpression enhanced the promoting effect of exogenous CGRP on osteogenic differentiation in MG‐63 cells. We first constructed a eukaryotic expression vector containing human RAMP1 and stably transfected it into MG‐63 cells. Real‐time PCR and Western blotting were used to determine the expression levels of RAMP1 and CRLR mRNA and protein, respectively. Immunofluorescence analysis was employed to compare the distribution of CRLR in transfected cells. After treatment with CGRP, the extent of osteogenic differentiation was evaluated by simultaneous monitoring of alkaline phosphatase activity, the expression patterns of osteoblastic markers and mineralisation staining. We found that RAMP1 was more highly expressed in the transfected group compared with the control groups (P < 0.01). The CRLR expression was significantly higher than that in the control groups (P < 0.05). In addition, after 7 days of CGRP treatment to induce osteogenic differentiation, the expression of collagen I mRNA was markedly increased in the transfected group (P < 0.05). The transfected group exhibited more granular precipitation in the cytoplasm with alkaline phosphatase staining after 7 and 14 days of differentiation. When stained with Alizarin Red, cells overexpressing RAMP1 were darker and formed many mineralised nodules with clear boundaries and calcium deposition typical of mineralised bone matrix structures at 28 days post‐induction of differentiation. The CGRP‐induced ALP activity in the RAMP1 overexpression group was significantly higher 3, 6 and 9 days after induction than that in the two control groups (P < 0.05). RAMP1 overexpression promotes CRLR expression, localisation on the cell membrane and enhanced CGRP‐mediated differentiation of MG‐63 cells. This study contributes to a better understanding of the molecular mechanisms governing CGRP‐induced MG‐63 differentiation. J. Cell. Biochem. 114: 314–322, 2013. © 2012 Wiley Periodicals, Inc.
Carcinogenicity and hormone studies with the tissue‐selective estrogen receptor modulator bazadoxifene
Authors: Wright David J. , Earnhardt J. Nicole , Perry Richard , Bailey Steven , Komm Barry , Minck Daniel R. , Cukierski Mark A. 
Published: September 2012

AbstractBazedoxifene Acetate (BZA) is a selective estrogen receptor modulator (SERM) that is approved for the prevention and/or treatment of osteoporosis in postmenopausal women. To assess for carcinogenic potential, BZA was administered ad libitum in the diet to rats for 2 years. BZA caused an increase in benign ovarian tumors in female rats and decreased incidences of mammary tumors (females) and pituitary tumors (males and females). In addition, BZA provided a significant survival benefit at all dosages tested, which correlated with a significant reduction in pituitary and mammary gland tumors and decreased body weight gain (both genders). Additional studies were subsequently conducted in rats and monkeys to further explore the mechanisms likely responsible for the observed effects. Results from studies in hypophysectomized and chemically castrated female rats indicated that BZA did not directly stimulate formation of ovarian cysts, but an intact pituitary was required for cyst formation. Further, BZA increased estradiol concentrations in rats and monkeys. In monkeys, BZA increased concentrations of luteinizing hormone (LH) after onset of treatment and prohibited the preovulatory surge of LH until after cessation of treatment. These hormonal changes suggest that BZA inhibited both the positive and negative feedback effects of estrogen on gonadotropins and the resulting increase in LH caused formation and persistence of ovarian cysts, which eventually transformed into benign ovarian granulosa cell tumors in the rat carcinogenicity study. These results also suggest that the reductions in pituitary and mammary gland tumors were attributed to BZA‐related antagonism of endogenous estrogens at the estrogen receptors. J. Cell. Physiol. 228: 724–733, 2013. © 2012 Wiley Periodicals, Inc.
Regulation of intracellular pH in cancer cell lines under normoxia and hypoxia
Authors: Hulikova Alzbeta , Harris Adrian L. , Vaughan‐Jones Richard D. , Swietach Pawel 
Published: September 2012

AbstractAcid‐extrusion by active transport is important in metabolically active cancer cells, where it removes excess intracellular acid and sets the intracellular resting pH. Hypoxia is a major trigger of adaptive responses in cancer, but its effect on acid‐extrusion remains unclear. We studied pH‐regulation under normoxia and hypoxia in eight cancer cell‐lines (HCT116, RT112, MDA‐MB‐468, MCF10A, HT29, HT1080, MiaPaca2, HeLa) using the pH‐sensitive fluorophore, cSNARF‐1. Hypoxia responses were triggered by pre‐incubation in low O2 or with the 2‐oxoglutarate‐dependent dioxygenase inhibitor dimethyloxalylglycine (DMOG). By selective pharmacological inhibition or transport‐substrate removal, acid‐extrusion flux was dissected into components due to Na+/H+ exchange (NHE) and Na+‐dependent HCO transport. In half of the cell‐lines (HCT116, RT112, MDA‐MB‐468, MCF10A), acid‐extrusion on NHE was the dominant flux during an acid load, and in all of these, bar one (MDA‐MB‐468), NHE‐flux was reduced following hypoxic incubation. Further studies in HCT116 cells showed that <4‐h hypoxic incubation reduced NHE‐flux reversibly with a time‐constant of 1–2 h. This was not associated with a change in expression of NHE1, the principal NHE isoform. Following 48‐h hypoxia, inhibition of NHE‐flux persisted but became only slowly reversible and associated with reduced expression of the glycosylated form of NHE1. Acid‐extrusion by Na+‐dependent HCO transport was hypoxia‐insensitive and comparable in all cell lines. This constitutive and stable element of pH‐regulation was found to be important for setting and stabilizing resting pH at a mildly alkaline level (conducive for growth), irrespective of oxygenation status. In contrast, the more variable flux on NHE underlies cell‐specific differences in their dynamic response to larger acid loads. J. Cell. Physiol. 228: 743–752, 2013. © 2012 Wiley Periodicals, Inc.
The pattern and risk factors of recurrence of proximal gastric cancer after curative resection
Authors: Li Fangxuan , Zhang Rupeng , Liang Han , Liu Hui , Quan Jichuan 
Published: September 2012

AbstractPurposeTo explore the time and pattern of recurrence of proximal gastric cancer and estimate the risk factors and prognostic factors for it. Considering these risk factors, postoperative adjuvant therapies and follow‐up program can be individualized.MethodsThe data of 135 recurrence proximal gastric cancer patients were extracted and analyzed.ResultsIn 135 recurrence patients, the median time to recurrence was 14.0 months, 116 (85.9%) patients had recurrences within 2 years. Loco‐regional recurrence was the most prevalent pattern. Hematogenous metastasis was next prevalent pattern in which the liver was the most common organ. Peritoneal recurrence occurred in 32 patients. Five patients recurred in distant lymph nodes. The deeper invasion was associated with higher incidence of hematogenous metastases and peritoneal recurrence. The histological type, depth of invasion, and lymph node metastasis were independent risk factors for overall recurrence. While, negative lymph nodes counts were another independent risk factors for early recurrence. Patients with systemic recurrence and early recurrence patients had poorer prognosis.ConclusionTotal gastrectomy and adequate lymph nodes dissection were rational choice of proximal gastric cancer with deeper invasion. Pathologic predictors of invasion, histological type, lymph nodes metastasis and negative lymph node counts could guide individualized, risk‐oriented adjuvant treatment, and follow‐up plan. J. Surg. Oncol. 2013;107:130–135. © 2012 Wiley Periodicals, Inc.
On the fundamental structure of femtosecond laser‐induced nanogratings
Authors: Richter S. , Plech A. , Steinert M. , Heinrich M. , Döring S. , Zimmermann F. , Peschel U. , Kley E.Bernhard , Tünnermann A. , Nolte S. 
Published: September 2012

AbstractThe nanoscale structure of femtosecond laser‐induced modifications known as “nanogratings” has been the subject of speculation and intensive debate throughout the decade since their discovery. The aim of this work is to gain dependable information on the three‐dimensional (3D) substructure of nanogratings down to the nanometre scale. To this end, non‐destructive small angle X‐ray scattering (SAXS) was employed to determine the characteristic sizes associated with the smallest features over a wide range of inscription parameters. The characteristic size of these cavities is 30 × 200 × 300 nm3 and largely independent of the exposure parameters, whereas prolonged exposure to laser pulses leads to an increase in their total number. Subsequently, focused ion beam (FIB) milling was used to dissect an extended volume and for the first time directly observe the 3D structure of nanogratings with nanometre resolution. The experiments clearly show that hollow cavities are the primary constituents of nanogratings and that their sheet‐like arrangement gives rise to the well‐known periodicity.
Effect of Nanoclay on in situ Preparation of “All Acrylate” ABA Triblock Copolymers via ATRP and Their Morphology
Authors: Singha Nikhil K. , Kavitha Amalin , Haloi Dhruba J. , Mandal Prithwiraj , Janke Andreas , Jehnichen Dieter , Komber Hartmut , Voit Brigitte 
Published: September 2012

AbstractThe preparation and characterization of a series of poly(methyl methacrylate)‐block‐poly(butyl acrylate)‐block‐poly(methyl methacrylate) ABA triblock copolymers and their clay nanocomposites via in situ ATRP is reported. The molecular weights and the chemical structures of these nanocomposites are characterized by means of GPC, FTIR, and NMR analyses. DSC, AFM, and SAXS analyses prove a nanophase‐separated morphology in the block copolymer/clay nanocomposites. DMA analysis demonstrates an additional reinforcing effect of nanoclay in the block copolymer nanocomposites. AFM analysis indicates that the bulk polymer has a cylindrical morphology that is unaffected in the nanocomposite, as corroborated by SAXS analysis.
Abundance and short‐term temporal variability of fecal microbiota in healthy dogs
Authors: Garcia‐Mazcorro Jose F. , Dowd Scot E. , Poulsen Jeffrey , Steiner Jörg M. , Suchodolski Jan S. 
Published: September 2012

AbstractTemporal variations of intestinal microorganisms have been investigated in humans, but limited information is available for other animal species. The aim of the study was to evaluate the abundance and short‐term temporal variability of fecal microbiota in dogs. Two fecal samples were collected (15 days apart) from six healthy dogs. The microbiota was evaluated using fluorescence in situ hybridization (FISH) and 454‐pyrosequencing targeting the 16S rRNA and its gene. Pyrosequencing revealed 15 families comprising >80% of all microbiota, over time intraindividual coefficients of variation (%CV) ranged from 2% to 141% (median: 55%). In contrast, the interindividual %CV ranged from 62% to 230% (median: 145%). Relative proportions of Faecalibacterium (important for intestinal health) and Subdoligranulum were low (two dogs harbored 4–7% of Subdoligranulum, the remaining dogs had <1% of either genus). Conversely, FISH revealed that Faecalibacterium comprised a median of 5% of total counts (range: 0–8%, probe Fprau645). A novel FISH probe (Faecali 698) was tested that, compared with Fprau645, can detect in silico a similar percentage of Faecalibacterium but higher proportions of Subdoligranulum. This probe revealed a high percentage of Faecalibacterium–Subdoligranulum (median: 16% of total counts). Future studies should consider the observed variability and discrepancies in microbial abundance between FISH and 454‐pyrosequencing.
Oroxylin a prevents inflammation‐related tumor through down‐regulation of inflammatory gene expression by inhibiting NF‐κB signaling
Authors: Yao Jing , Hu Rong , Sun Jie , Lin Biqi , Zhao Li , Sha Yunying , Zhu Binbin , You Qi‐Dong , Yan Tianhua , Guo Qing‐Long 
Published: September 2012

AbstractIncreasing evidence suggests that inflammatory microenvironment plays a critical role at different stages of tumor development. However, the molecular mechanisms of the interaction between inflammation and proliferation of cancer cells remain poorly defined. Here we reported the inhibitory effects of oroxylin A on the inflammation‐stimulated proliferation of tumor cells and delineated the mechanism of its action. The results indicated that treatment with oroxylin A inhibited NF‐κB p65 nuclear translocation and phosphorylation of IκBα and IKKα/β in both human colon tumor HCT116 cells and human monocytes THP‐1 cells. In addition, in THP‐1 cells, oroxylin A significantly suppressed lipopolysaccharide (LPS)‐induced secretion of prototypical proinflammatory cytokine IL‐6 but not IL‐1β, and it was confirmed at the transcription level. Moreover, oroxylin A inhibited the proliferation of HCT116 cells stimulated by LPS‐induced THP‐1 cells in co‐culture microenvironment. In summary, oroxylin A modulated NF‐κB signaling pathway involved in inflammation‐induced cancer initiation and progression and therefore could be a potential cancer chemoprevention agent for inflammation‐related cancer. © 2012 Wiley Periodicals, Inc.
Dose‐dependent absorption of chlorogenic acids in the small intestine assessed by coffee consumption in ileostomists
Authors: Erk Thomas , Williamson Gary , Renouf Mathieu , Marmet Cynthia , Steiling Heike , Dionisi Fabiola , Barron Denis , Melcher Ralf , Richling Elke 
Published: September 2012

ScopeUntil now, the question of how the ingested doses of chlorogenic acids (CGA) from coffee influence their absorption and metabolism remains unresolved. To assess absorption in the small intestine, we performed a dose‐response study with a randomized, double‐blinded, crossover design with ileostomist subjects.Methods and resultsAfter a polyphenol‐free diet, the volunteers consumed, on three separate occasions, coffee with different total CGA contents (high 4525 μmol; medium 2219 μmol; low 1053 μmol). CGA concentrations in plasma, ileal effluent, and urine were subsequently determined by HPLC‐DAD‐ESI‐MS and ‐ESI‐MS/MS. The results show that the consumption of higher CGA concentrations leads to a faster ileal excretion. This corresponds to a renal excretion of 8.0 ± 4.9% (high), 12.1 ± 6.7% (medium), and 14.6 ± 6.8% (low) of total CGA and metabolites. Glucuronidation of CGA became slightly greater with increasing dose. After enzyme treatment, the area under the curve (AUC)0–8h for CGA metabolites in plasma was 4412 ± 751 nM × h0–8−1 (high), 2394 ± 637 nM × h0–8−1 (medium), 1782 ± 731 nM × h0–8−1 (low), respectively. Additionally, we were able to identify new metabolites of CGA in urine and ileal fluid.ConclusionWe conclude that the consumption of high CGA concentrations via coffee might influence the gastrointestinal transit time and consequently affect CGA absorption and metabolism.