bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of AFLP and SSR markers linked with the male fertility restorer gene of CMS 06J45 in heading Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Authors: Xu Xiaoyong , Sun Xilu , Zhang Yu , Zhang Lugang , Fang Zhiyuan 
Published: June 2014

AbstractIn this study, AFLP and SSR techniques were combined with the bulk segregant analysis (BSA) method to map the restorer gene BrRfp using an F2‐segregating population comprising 258 individuals developed by crossing the polima (pol)‐like cytoplasmic male sterility (CMS) line 06J45 and the restorer line 01S325 of heading Chinese cabbage. A survey of 2048 AFLP primer pairs identified 21 polymorphic fragments, approximately half of which exhibited high similarity with the A09 chromosome sequence of Brassica rapa in the Brassica database (BRAD). Based on the genome sequence, three specific AFLP fragments linked with BrRfp were successfully converted into sequence‐characterized amplified region (SCAR) markers, named SC1233, SC2673 and SC2141. Subsequently, 178 pairs of SSR primers were redesigned for further screening, with five producing polymorphic amplification patterns. Linkage analysis showed that these markers were distributed along both sides of the BrRfp gene, with two markers, SSR03 and SSR2528, co‐segregating with the BrRfp locus in the F2 population. These results may be valuable for marker‐assisted selection and map‐based cloning in heading Chinese cabbage.
Using the Fisher–Shannon method to characterize continuous seismic signal during volcanic eruptions: application to 2011–2012 El Hierro (Canary Islands) eruption
Authors: Telesca Luciano , Lovallo Michele , Martì Molist Joan , López Moreno Carmen , Abella Meléndez Rafael 
Published: June 2014

AbstractWe investigated the dynamics of the continuous seismic signal recorded before and during the 2011–2012 El Hierro eruption (Canary Islands) using the innovative approach of the Fisher–Shannon method, a suitable statistical tool for detecting dynamic changes in complex systems. Our findings identify dynamic changes in the seismic signal that can be correlated with different stress states of the magmatic setting and the plumbing system in the volcano at El Hierro. The results contribute to the understanding of the fracturing pattern in the crust during a new intrusion and eruption of an overpressurized batch of magma.
SAGA/ADA Complex Subunit Ada2 Is Required for Cap1- but Not Mrr1-Mediated Upregulation of the Candida albicans Multidrug Efflux Pump
MDR1
Authors: Ramírez-Zavala Bernardo , Mogavero Selene , Schöller Eva , Sasse Christoph , Rogers P. David , Morschhäuser Joachim 
Published: June 2014

ABSTRACT

Overexpression of the multidrug efflux pump
MDR1
is one mechanism by which the pathogenic yeast
Candida albicans
develops resistance to the antifungal drug fluconazole. The constitutive upregulation of
MDR1
in fluconazole-resistant, clinical
C. albicans
isolates is caused by gain-of-function mutations in the zinc cluster transcription factor Mrr1. It has been suggested that Mrr1 activates
MDR1
transcription by recruiting Ada2, a subunit of the SAGA/ADA coactivator complex. However,
MDR1
expression is also regulated by the bZIP transcription factor Cap1, which mediates the oxidative stress response in
C. albicans
. Here, we show that a hyperactive Mrr1 containing a gain-of-function mutation promotes
MDR1
overexpression independently of Ada2. In contrast, a C-terminally truncated, hyperactive Cap1 caused
MDR1
overexpression in a wild-type strain but only weakly in mutants lacking
ADA2
. In the presence of benomyl or H
2
O
2
, compounds that induce
MDR1
expression in an Mrr1- and Cap1-dependent fashion,
MDR1
was upregulated with the same efficiency in wild-type and
ada2
Δ cells. These results indicate that Cap1, but not Mrr1, recruits Ada2 to the
MDR1
promoter to induce the expression of this multidrug efflux pump and that Ada2 is not required for
MDR1
overexpression in fluconazole-resistant
C. albicans
strains containing gain-of-function mutations in Mrr1.
Contribution of the Highly Conserved EaeH Surface Protein to Enterotoxigenic Escherichia coli Pathogenesis
Authors: Sheikh Alaullah , Luo Qingwei , Roy Koushik , Shabaan Salwa , Kumar Pardeep , Qadri Firdausi , Fleckenstein James M. 
Published: June 2014

ABSTRACT

Enterotoxigenic
Escherichia coli
(ETEC) strains are among the most common causes of diarrheal illness worldwide. These pathogens disproportionately afflict children in developing countries, where they cause substantial morbidity and are responsible for hundreds of thousands of deaths each year. Although these organisms are important targets for enteric vaccines, most development efforts to date have centered on a subset of plasmid-encoded fimbrial adhesins known as colonization factors and heat-labile toxin (LT). Emerging data suggest that ETEC undergoes considerable changes in its surface architecture, sequentially deploying a number of putative adhesins during its interactions with the host. We demonstrate here that one putative highly conserved, chromosomally encoded adhesin, EaeH, engages the surfaces of intestinal epithelial cells and contributes to bacterial adhesion, LT delivery, and colonization of the small intestine.
Selective Activity of the Histone Deacetylase Inhibitor AR-42 against Leukemia Stem Cells: A Novel Potential Strategy in Acute Myelogenous Leukemia
Authors: Guzman Monica L. , Yang Neng , Sharma Krishan K. , Balys Marlene , Corbett Cheryl A. , Jordan Craig T. , Becker Michael W. , Steidl Ulrich , Abdel-Wahab Omar , Levine Ross L. , Marcucci Guido , Roboz Gail J. , Hassane Duane C. 
Published: June 2014

AbstractMost patients with acute myelogenous leukemia (AML) relapse and die of their disease. Increasing evidence indicates that AML relapse is driven by the inability to eradicate leukemia stem cells (LSC). Thus, it is imperative to identify novel therapies that can ablate LSCs. Using an in silico gene expression–based screen for compounds evoking transcriptional effects similar to the previously described anti-LSC agent parthenolide, we identified AR-42 (OSU-HDAC42), a novel histone deacetylase inhibitor that is structurally similar to phenylbutyrate, but with improved activity at submicromolar concentrations. Here, we report that AR-42 induces NF-κB inhibition, disrupts the ability of Hsp90 to stabilize its oncogenic clients, and causes potent and specific cell death of LSCs but not normal hematopoietic stem and progenitor cells. Unlike parthenolide, the caspase-dependent apoptosis caused by AR-42 occurs without activation of Nrf-2–driven cytoprotective pathways. As AR-42 is already being tested in early clinical trials, we expect that our results can be extended to the clinic. Mol Cancer Ther; 13(8); 1979–90. ©2014 AACR.
Gammaglobulins in critical care
Authors: Carmona Fabio 
Published: June 2014

The use of intravenous immunoglobulin (IVIG) is relatively infrequent in patients admitted to intensive care units (ICUs). However, “off-label” IVIG prescriptions for different conditions are highly prevalent. The aim of this paper is to review the existing evidence for the use of IVIG in patients admitted to ICUs, emphasizing non-infectious diseases and complications: hypogammaglobulinemia of the critically ill,hemophagocytic lymphohistiocytosis (HLH), Guillain-Barré syndrome (GBS), Kawasaki disease (KD), chylothorax, acute myocarditis, toxic shock syndrome (TSS), Stevens-Johnson syndrome (SJS)/toxic epidermal necrolysis (TEN), and sepsis.In conclusion, in critically ill patients, IVIG use is of benefit in KD, GBS, and TSS. It may benefit patients with fulminant acute myocarditis. The benefit is not proven in patients with HLH, chylothorax, and SJS/TEN.
On Argentocypris sara gen. nov., sp. nov. (Ostracoda) from the Patagonian wetlands of Argentina
Authors: Díaz Analia R. , Martens Koen 
Published: June 2014

We describe Argentocypris sara, a new genus and species from a Patagonian lake in the province of Neuquén, Argentina. The genus and species clearly belong to the tribe Eucypridini, and as such have a rather isolated biogeographical position in South America. The genus can be distinguished from other Eucypridini based on both valve (large external Porenwarzen, anterior selvage and posterior inner list on RV) and soft part features (cylindrical shape of second distal segment of Mx1-palp, length ratio setae d1 = c 3 × d2 on T2, curved attachment of caudal ramus). A study of the literature reveals that several other nominal species can be transferred to this genus. Nevertheless, at least two of these species (A. virgata n. comb. and A. labyrinthica n. comb.) are based on juvenile fossil specimens only and their identity is uncertain. Two other species (A. fontana n. comb. and A. sarsi n. comb.) are based on adult morphology.
Clouds, Aerosols, and Precipitation in the Marine Boundary Layer: An Arm Mobile Facility Deployment
Authors: Wood Robert , Wyant Matthew , Bretherton Christopher S. , Rémillard Jasmine , Kollias Pavlos , Fletcher Jennifer , Stemmler Jayson , de Szoeke Simone , Yuter Sandra , Miller Matthew , Mechem David , Tselioudis George , Chiu J. Christine , Mann Julian A. L. , O’Connor Ewan J. , Hogan Robin J. , Dong Xiquan , Miller Mark , Ghate Virendra , Jefferson Anne , Min Qilong , Minnis Patrick , Palikonda Rabindra , Albrecht Bruce , Luke Ed , Hannay Cecile , Lin Yanluan 
Published: June 2014

Abstract
The Clouds, Aerosol, and Precipitation in the Marine Boundary Layer (CAP-MBL) deployment at Graciosa Island in the Azores generated a 21-month (April 2009–December 2010) comprehensive dataset documenting clouds, aerosols, and precipitation using the Atmospheric Radiation Measurement Program (ARM) Mobile Facility (AMF). The scientific aim of the deployment is to gain improved understanding of the interactions of clouds, aerosols, and precipitation in the marine boundary layer.
Graciosa Island straddles the boundary between the subtropics and midlatitudes in the northeast Atlantic Ocean and consequently experiences a great diversity of meteorological and cloudiness conditions. Low clouds are the dominant cloud type, with stratocumulus and cumulus occurring regularly. Approximately half of all clouds contained precipitation detectable as radar echoes below the cloud base. Radar and satellite observations show that clouds with tops from 1 to 11 km contribute more or less equally to surface-measured precipitation at Graciosa. A wide range of aerosol conditions was sampled during the deployment consistent with the diversity of sources as indicated by back-trajectory analysis. Preliminary findings suggest important two-way interactions between aerosols and clouds at Graciosa, with aerosols affecting light precipitation and cloud radiative properties while being controlled in part by precipitation scavenging.
The data from Graciosa are being compared with short-range forecasts made with a variety of models. A pilot analysis with two climate and two weather forecast models shows that they reproduce the observed time-varying vertical structure of lower-tropospheric cloud fairly well but the cloud-nucleating aerosol concentrations less well. The Graciosa site has been chosen to be a permanent fixed ARM site that became operational in October 2013.
Sudden neuropathological deaths: An autopsy study
Authors: Hugar Basappa Subhas , Shetty Harish , Girishchandra YP , Hosahally Jayanth S 
Published: June 2014

Background Sudden or unexpected death can occur from unnatural causes, such as violence or poisoning, as well as from natural causes. Second to cardiac causes, neuropathology is one of the main causes of sudden natural death. In spite of the increasing incidence of neuropathological deaths, few studies have been conducted in Asia – hence the reason for the present study. Methods A 10-year (January 2003 to December 2012) retrospective study was conducted at the MS medico-legal institute in a metropolitan city of southern India. All the cases of sudden natural death, where the cause of death was opined to be due to a neuropathology after meticulous medico-legal autopsy and chemical analysis, were included. Results and conclusions A total of 7520 reported deaths were investigated. These included 291 sudden natural deaths, amongst which the death was attributed to neuropathology in 43 cases (14.7%). Of these 43 cases, 69.7% were male and 30.3% were female. The majority of cases (25.5%) were in those aged ≥60 years. Causes of neuropathology were due to subarachnoid haemorrhage in 48.8% of cases, stroke in 30.2%, meningitis in 14% and tumours in 7%. There was history of hypertension in 46.5% of cases, diabetes in 32.5% of cases and cardiac disease in 18.6% of cases.
Cost‐benefit Analysis of Polysomnography versus Clinical Assessment Score‐15 (CAS‐15) for Treatment of Pediatric Sleep‐disordered Breathing
Authors: Konka Anita , Weedon Jeremy , Goldstein Nira A. 
Published: June 2014

ObjectiveTo determine the cost of medical care using the Clinical Assessment Score‐15 (CAS‐15) scale versus polysomnography (PSG) for children with sleep‐disordered breathing in terms of benefit.Study DesignCost‐benefit analysis.SettingHospital‐based pediatric otolaryngology practice.Subjects and MethodsNinety‐three patients from our original CAS‐15 study were included. Four clinical measures were used and payment data were obtained. Cost‐benefit analysis was performed for 2 clinical pathways. In pathway 1, all children had PSG; those with positive studies were referred for adenotonsillectomy. In pathway 2, children with CAS‐15 ≥ 32 were referred for adenotonsillectomy regardless of PSG. Paired t test compared intrasubject mean total cost (pathway 1 vs pathway 2). Further analyses computed a change score for the clinical measures (follow‐up minus baseline); these were divided by estimated treatment cost, producing 4 cost‐benefit ratios for each pathway. Paired t tests compared the mean of these ratios between the pathways.ResultsOf 65 PSG+ (15 CAS–), 54 underwent surgery; of 28 PSG– (17 CAS–), 7 underwent surgery. Model estimated costs demonstrate a mean cost benefit of $US1172 (SE = $214) for pathway 2 versus pathway 1 (P <. 001). CAS‐15 is also cost‐beneficial versus PSG in 3 of 4 clinical measures (Child Behavior Checklist total problem T score, P =. 008, mean OSA‐18 survey score, P <. 001, apnea hypopnea index, P <. 001).ConclusionsWe present evidence that a CAS‐15–based treatment decision criterion is superior to PSG in terms of monetary cost and in benefit per unit cost for 3 of 4 clinical measures evaluated.
A neutrophil intrinsic impairment affecting Rab27a and degranulation in cystic fibrosis is corrected by CFTR potentiator therapy
Authors: Pohl Kerstin , Hayes Elaine , Keenan Joanne , Henry Michael , Meleady Paula , Molloy Kevin , Jundi Bakr , Bergin David A. , McCarthy Cormac , McElvaney Oliver J. , White Michelle M. , Clynes Martin , Reeves Emer P. , McElvaney Noel G. 
Published: June 2014

Key PointsIn people with cystic fibrosis, defective CFTR function alters neutrophil cytosolic ion homeostasis leading to impaired degranulation. By normalizing CFTR function, ivacaftor treatment corrects neutrophil degranulation resulting in normalized killing of bacteria.
Spinal cord stimulation for complex regional pain syndrome type 1 with dystonia: a case report and discussion of the literature
Authors: Voet Caroline , le Polain de Waroux Bernard , Forget Patrice , Deumens Ronald , Masquelier Etienne 
Published: June 2014

Background: Complex Regional Pain Syndrome type 1 (CRPS-1) is a debilitating chronic pain disorder, the physiopathology of which can lead to dystonia associated with changes in the autonomic, central and peripheral nervous system. An interdisciplinary approach (pharmacological, interventional and psychological therapies in conjunction with a rehabilitation pathway) is central to progress towards pain reduction and restoration of function.Aim: This case report aims to stimulate reflection and development of mechanism-based therapeutic strategies concerning CRPS associated with dystonia.Case description: A 31 year old female CRPS-1 patient presented with dystonia of the right foot following ligamentoplasty for chronic ankle instability. She did not have a satisfactory response to the usual therapies. Multiple anesthetic blocks (popliteal, epidural and intrathecal) were not associated with significant anesthesia and analgesia. Mobilization of the foot by a physiotherapist was not possible. A multidisciplinary approach with psychological support, physiotherapy and spinal cord stimulation (SCS) brought pain relief, rehabilitation and improvement in the quality of life.Conclusion: The present case report demonstrates the occurrence of multilevel (peripheral and central) pathological modifications in the nervous system of a CRPS-1 patient with dystonia. This conclusion is based on the patient’s pain being resistant to anesthetic blocks at different levels and the favourable, at least initially, response to SCS. The importance of the bio-psycho-social model is also suggested, permitting behavioural change.
Congenital duplication of the urethra with urethral diverticulum: a case report
Authors: Shah Darshan H , Ganpule Arvind P , Sabnis Ravindra B , Desai Mahesh R 
Published: June 2014

Duplication of the urethra is a rare congenital anomaly. Urethral duplication with the presence of diverticulum is a rare combination and to the best of our knowledge has not been previously reported. We report a case of a 16 month old male child with duplication of the urethra and diverticulum arising from the ventral urethra. We also cover the intricacies and challenges in the management of such a case.The opening of the narrowed accessory dorsal urethra at the verumontanum was cauterized and gradually the dorsal urethra became atrophied. The ventral urethral diverticulum was excised. This case is unique due to:The unusual presentation of swelling over the dorsum of the penis, together with duplication of the urethra with diverticulum.The use of cauterization as a treatment modality. Cauterization of the ventral urethra with a Bugbee electrode and diverticulectomy was performed. A glidewire helped in identifying the small opening of the dorsal urethra at the level of the verumontanum. The case also highlights the importance of endoscopic management of this clinical entity.
What It's Like to Grow Older: The Aging Perceptions of People With an Intellectual Disability in Ireland
Authors: Burke Eilish , McCarron Mary , Carroll Rachael , McGlinchey Eimear , McCallion Philip 
Published: June 2014

AbstractThe Intellectual Disability Supplement to The Irish Longitudinal Study on Ageing is a national longitudinal study on the aging of people with an intellectual disability (ID) using a randomly selected sample of people with ID over the age of 40. In total, 367 people with an ID completed the aging perception self-report only section. Over 57% of people described their health as very good to excellent with no significant difference in health perceptions found for gender, level of ID, or living circumstance. Exploring people's perceptions utilizing PASW Text Analytics for Surveys 4.1 perceptions often supported negative views of the consequences of aging. These findings suggest challenging negative aging concepts is essential to promote positivity with associated improved health and wellbeing.
The ‘terrorism’ frame in ‘neo-Orientalism’: Western news and the Sunni–Shia Muslim sectarian relations after 9/11
Authors: Douai Aziz , Lauricella Sharon 
Published: June 2014

Abstract
Sunni–Shia relations have become a topic of significant media attention; this attention is largely due to the tendency of these groups to engage in sectarian strife as well as the Islamic Shia sect’s influential rise in world politics. The inter-Islamic sectarian relations are part of extensive reporting on upheaval events currently taking place in the Greater Middle East in the post 9/11 decade. This study analyses ten years of news coverage of the Sunni–Shia relations in the Canadian Globe and Mail and the US-based Washington Post. Results indicate that rather than contextualizing sectarian tensions between Sunni and Shia Islam, this media coverage overwhelmingly frames the tensions from the ‘war on terrorism’ perspective. This article argues that ‘neo-Orientalist’ discourses and propagandist perspectives have become routinized and more prevalent in the manner in which Western media represents Islam, privileging dominant discourses and ‘war on terrorism’ frames.
Modeling the Effects of the Quantity and Particle Size of Wheat Bran on Some Properties of Bread Dough using Response Surface Methodology
Authors: Majzoobi Mahsa , Pashangeh Safoora , Aminlari Ladan , Farahnaky Asgar 
Published: June 2014

Abstract
The aim of this study was to find empirical models for some properties of bread dough as a function of bran concentration and particle size using D-Optimal made by Design Expert Software. Wheat flour was replaced with 0, 5, 10, 15 and 20% (w/w, flour basis) wheat bran at particle sizes of 170, 280, 425 and 750 μm. Increasing bran concentration and particle size enhanced dough water absorption. Dough arrival and development times increased, while dough stability time, tolerance to mixing and volume rising decreased as the bran particle size and concentration increased. The dough became darker, more reddish and yellower with increasing bran concentration and particle size. Dough hardness increased while cohesiveness decreased with addition of bran concentration and particle size. In general, strong correlations between dough properties with bran quantity and particle size were established. Bran concentration had stronger effect on dough properties compared to its particle size.
Advances in understanding the transformation of engineered nanoparticles in the environment
Authors: Nick Setare Tahmasebi , Bolandi Ali , Samuels Tova A. , Obare Sherine O. 
Published: June 2014

AbstractEngineered nanoparticles (ENPs) are known to possess unique size and shape dependent chemical and physical properties. As a result of their properties, ENPs have been effective in several important applications including catalysis, sensor design, photonics, electronics, medicine, and the environmental remediation of toxic pollutants. Such properties and applications have led to an increase in the manufacture of ENPs and a rise in their presence in consumer products. The increase of ENPs in consumer products presents several opportunities and challenges, and necessitates a proactive study of their health and safety. This article highlights some recent work in which we have studied the effect of exposure of well-defined ENPs to pesticides and the effect of pH and dissolved organic matter. We also summarize our work and that of others who have studied the toxicity of ENPs with microorganisms. The results provide insights on the need for green manufacturing strategies of ENPs, their use and safe disposal practices.