bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neglected synovial osteochondromatosis of the elbow: a rare case
Authors: Giannetti Silvio , Santucci Attilio , Patricola Alessandro , Stancati Andrea , Di Sanzo Vincenzo 
Published: October 2013

AbstractBackgroundSynovial osteochondromatosis is a benign metaplastic proliferative disorder of the synovium characterised by the formation of multiple cartilaginous nodules in the synovium, many of which detach and become loose bodies. The disease is characteristically monoarticular, most commonly involving the knee. A site in the elbow was first reported in 1918 by Henderson, but any joint may be involved. Very few cases of synovial osteochondromatosis of the elbow have been reported in the literature. The presenting symptoms are usually diffuse discomfort in the affected joint and decreased range of motion with an accompanying gritty or locking sensation. The treatment of choice is excision of the synovium and removal of the loose bodies.Case presentationWe report a rare neglected case covering a 32-year period of a locally aggressive synovial osteochondromatosis of the elbow in a 47-year-old man. Clinical examination revealed a significant increase in size of the left elbow compared to the contralateral one. The simple radiographs and the computed tomography showed multiple rounded, calcified bodies widespread throughout the elbow joint. At surgery we removed and counted a total of 312 loose bodies, varying in size from a few millimeters to 3 cm. The evaluation at 6 months postoperatively showed marked reduction in the volume of the elbow, improvement of extension and flexion and an increase of the Mayo elbow performance score from 50 points before surgery to 80 points at 6 months postoperative.ConclusionSynovial osteochondromatosis is an uncommon condition characterized by the formation of multiple nodules of hyaline cartilage within the sub-synovial connective tissue. The differential diagnosis includes chronic articular infection, osteoarthritis, pigmented villonodular synovitis, mono-articular inflammatory arthritis and periarticular neoplasms like synovial sarcoma. The treatment of choice is excision of the synovium and removal of the loose bodies. The prognosis is good, but recurrences may occur if the removal is incomplete.
IGF-I increases markers of osteoblastic activity and reduces bone resorption via osteoprotegerin and RANK-ligand
Authors: Guerra-Menéndez Lucia , Sádaba Maria C , Puche Juan E , Lavandera Jose L , de Castro Luis F , de Gortázar Arancha R , Castilla-Cortázar Inma 
Published: October 2013

AbstractBackgroundBone is one of the major target tissues for Insulin-like Growth Factor I (IGF-I). Low doses of IGF-I were able to improve liver-associated osteopenia. In the present work, a model of partial IGF-I deficiency was used in order to provide insight into the mechanisms of the beneficial actions of IGF-I replacement therapy in bone.MethodsSeveral proteins involved in osteoblastic/osteocyte and osteoclastic differentiation and activity were studied in the three experimental groups: control (CO) group (wild type mice,Igf+/+, n = 10), heterozygousIgf+/-group with partial IGF-I deficiency (Hz, n = 10), and heterozygousIgf+/-mice treated with IGF-I for 10 days (Hz + IGF-I, n = 10).ResultsData in this paper confirm that the simple partial IGF-I deficiency is responsible for osteopenia, determined by densitometry and histopathology. These findings are associated with a reduced gene expression of osteoprotegerin, sclerostin, calcitonin receptor (CTR), insulin-like growth factor binding protein 5 and RUNX2. IGF-I replacement therapy normalized CTR gene expression and reduced markers of osteoclastic activity.ConclusionsLow doses of IGF-I constituted a real replacement therapy that normalized IGF-I serum levels improving the expression of most of these proteins closely involved in bone-forming, and reducing bone resorption by mechanisms related to osteoprotegerin, RANKL and PTH receptor.
Compressibility of porous TiO2 nanoparticle coating on paperboard
Authors: Stepien Milena , Saarinen Jarkko J , Teisala Hannu , Tuominen Mikko , Haapanen Janne , Mäkelä Jyrki M , Kuusipalo Jurkka , Toivakka Martti 
Published: October 2013

Abstract
Compressibility of liquid flame spray-deposited porous TiO2 nanoparticle coating was studied on paperboard samples using a traditional calendering technique in which the paperboard is compressed between a metal and polymer roll. Surface superhydrophobicity is lost due to a smoothening effect when the number of successive calendering cycles is increased. Field emission scanning electron microscope surface and cross‒sectional images support the atomic force microscope roughness analysis that shows a significant compressibility of the deposited TiO2 nanoparticle coating with decrease in the surface roughness and nanoscale porosity under external pressure.

PACS

61.46.-w; 68.08.Bc; 81.07.-b
Gq Rather than G11 Preferentially Mediates Nociceptor Sensitization
Authors: Wirotanseng Laura Nikita , Kuner Rohini , Tappe-Theodor Anke 
Published: October 2013

Background The Gq/11-protein signaling mechanism is essential throughout the nervous system, but little is known about the contribution of the individual G-protein GPCR signaling branches towards nociceptor activation and their specific role on nociceptor sensitization. We aimed to unravel the contribution of the Gq/11-signaling pathway towards nociceptor activation via a variety of classical inflammatory mediators signalling via different G-protein GPCRs and investigated the specific contribution of the individual Gq and G11, G-Proteins in nociceptors. Findings Using different transgenic mouse lines, lacking Gαq, Gα11, or both α-subunit of the G-proteins in primary nociceptive neurons, we analyzed the mechanical- and heat-sensitivity upon application of different GPCR-agonists that are known to play an important role under inflammatory conditions (e.g. ATP, Glutamate, Serotonin etc.). We found that the Gq/11-GPCR signaling branch constitutes a primary role in the manifestation of mechanical allodynia and a minor role in the development of thermal hyperalgesia. Moreover, with respect to the mediators used here, the Gq-protein is the principle G-protein among the Gq/11-protein family in nociceptive neurons leading to nociceptor sensitization. Conclusions Our results demonstrate that the Gq/11 signaling branch plays a primary role in nociceptor sensitization upon stimulation with classical GPCR ligands, contributing primarily towards the development of mechanically allodynia. Moreover, the deletion of the individual G-proteins led to the finding that the Gq-protein dominates the signalling machinery of the Gq/11 family of G-proteins in nociceptive neurons.
A comparative study on the traditional Indian Shodhana and Chinese processing methods for aconite roots by characterization and determination of the major components
Authors: Jaiswal Yogini , Liang Zhitao , Yong Peng , Chen Hubiao , Zhao Zhongzhen 
Published: October 2013

Abstract

Background
Aconitum is an indispensable entity of the traditional medicine therapy in Ayurveda and Traditional Chinese medicine (TCM), in spite of its known fatal toxicity characteristics. The prolonged use of this drug, irrespective of its known lethal effects, is governed by the practice of effective detoxification processes that have been used for decades. However, the processing methods of Ayurveda and TCM are different, and no comparative study has been carried out to evaluate their differences.
The objective of the present study was to carry out comparative chemical profiling of the roots of Aconitum heterophyllum Wall, A. carmichaelii Debx., and A. kusnezoffii Reichb. after application of two detoxification methods used in Ayurveda and one method used in TCM .

Results
Analysis of the processed samples was carried out by ultra-high performance liquid chromatography combined with quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS). The results obtained in the study demonstrate that all three processing methods used in Ayurveda and TCM effectively extract the diester diterpenoid alkaloids and led to their conversion into monoester diterpenoid alkaloids. The efficiency of the processes in reduction of toxic alkaloid contents can be stated as: Processing with water > Shodhana with cow milk > Shodhana with cow urine. The analysis method was validated as per ICH-Q2R1 guidelines and all the parameters were found to comply with the recommendations stated in the guidelines.

Conclusions
There have been no reports till date, to compare the processing methods used in Ayurveda with the methods used in TCM for detoxification of aconite roots. Our study demonstrates that, these methods used in both the traditional systems of medicine, efficiently detoxify the aconite roots. Amongst the three selected procedures, the TCM method of decoction with water is the most efficient. Through experimental evidences, we prove the conversion of toxic diester diterpenoid alkaloids to relatively safer monoester diterpenoid alkaloids. Thus, this study demonstrates that comparative study on the traditional experiences accumulated in different medical systems is useful for expanding their respective applications.
Immunopurification of adenomatous polyposis coli (APC) proteins
Authors: Elliott Kerryn L , Catimel Bruno , Church Nicole L , Coates Janine L , Burgess Antony W , Layton Meredith J , Faux Maree C 
Published: October 2013

AbstractBackgroundTheadenomatous polyposis coli(APC) tumour suppressor gene encodes a 2843 residue (310 kDa) protein. APC is a multifunctional protein involved in the regulation of β-catenin/Wnt signalling, cytoskeletal dynamics and cell adhesion.APCmutations occur in most colorectal cancers and typically result in truncation of the C-terminal half of the protein.ResultsIn order to investigate the biophysical properties of APC, we have generated a set of monoclonal antibodies which enable purification of recombinant forms of APC. Here we describe the characterisation of these anti-APC monoclonal antibodies (APC-NT) that specifically recognise endogenous APC both in solution and in fixed cells. Full-length APC(1–2843) and cancer-associated, truncated APC proteins, APC(1–1638) and APC(1–1311) were produced in Sf9 insect cells.ConclusionsRecombinant APC proteins were purified using a two-step affinity approach using our APC-NT antibodies. The purification of APC proteins provides the basis for detailed structure/function analyses of full-length, cancer-truncated and endogenous forms of the protein.
Comparative proteomic analysis reveals mechanistic insights into Pseudomonas putida F1 growth on benzoate and citrate
Authors: Mandalakis Manolis , Panikov Nicolai , Dai Shujia , Ray Somak , Karger Barry L 
Published: October 2013

Abstract

Pseudomonas species are capable to proliferate under diverse environmental conditions and thus have a significant bioremediation potential. To enhance our understanding of their metabolic versatility, this study explores the changes in the proteome and physiology of Pseudomonas putida F1 resulting from its growth on benzoate, a moderate toxic compound that can be catabolized, and citrate, a carbon source that is assimilated through central metabolic pathways. A series of repetitive batch cultivations were performed to ensure a complete adaptation of the bacteria to each of these contrasting carbon sources. After several growth cycles, cell growth stabilized at the maximum level and exhibited a reproducible growth profile. The specific growth rates measured for benzoate (1.01 ± 0.11 h-1) and citrate (1.11 ± 0.12 h-1) were similar, while a higher yield was observed for benzoate (0.6 and 0.3 g cell mass per g of benzoate and citrate, respectively), reflecting the different degrees of carbon reduction in the two substrates. Comparative proteomic analysis revealed an enrichment of several oxygenases/dehydrogenases in benzoate-grown cells, indicative of the higher carbon reduction of benzoate. Moreover, the upregulation of all 14 proteins implicated in benzoate degradation via the catechol ortho-cleavage pathway was observed, while several stress-response proteins were increased to aid cells to cope with benzoate toxicity. Unexpectedly, citrate posed more challenges than benzoate in the maintenance of pH homeostasis, as indicated by the enhancement of the Na+/H+ antiporter and carbonic anhydrase. The study provides important mechanistic insights into Pseudomonas adaptation to varying carbon sources that are of great relevance to bioremediation efforts.
On seismic deghosting by spatial deconvolution
Authors: Amundsen Lasse , Zhou Hongbo , Reitan Arne , Weglein Arthur B. 
Published: October 2013

Receiver-side deghosting can be derived and implemented in the frequency domain as spatial deterministic deconvolution of marine pressure recordings. The deghosting/deconvolution operator is found analytically as the inverse Fourier transform of the wavenumber-domain wave equation deghosting function. For a sea surface reflection coefficient of [Formula: see text], the wavenumber-domain deghosting function has well-known poles at fundamental frequencies equal to an integer multiple of a function of the receiver depth and the plane-wave dip angle relative to the depth axis. The first singularity is always at 0 Hz. The spatial deghosting operator has singularities at fundamental frequencies equal to an integer multiple of a function of the receiver depth, independent of its lateral coordinates. The first singularity is again at 0 Hz. In addition, the deghosting operator that is applied to 3D data has singularity when its lateral coordinate is zero. A simple numerical example demonstrates the method.
Addressee’s Pursuasive Tactics in PR-Texts
Authors: Троицкая  , Troitskaya Tatyana 
Published: October 2013

The paper dwells upon the pragmatics of the political PR-text in the
communicative environment of the Internet, the main advantages of which
are multimedia and hypertextuality. Skillful constructing of the social reality
in PR-discourse directs addressee’s perception in the proper way. The
author analyses discursive strategies of presentation and politicians’ selfpresentation,
his opponents’ discredit on the basis of German pre-elective
PR-texts; singles out and describes addressee’s persuasive tactics and
their linguistic realization.
News coverage of the 2010 Iraqi general elections
Authors: Al-Rawi Ahmed K. , Gunter Barrie 
Published: October 2013

Abstract
This study investigates the tone of election news coverage in the evening newscasts of four Iraqi satellite TV channels: Iraqia (government-run), Furat (Shiite), Hurria (Kurdish) and Baghdad (Sunni) for two weeks leading up to the general election. A total sample of 56 evening newscasts containing 857 news stories that were related to the election was analysed. Although all channels claimed to be addressing all Iraqis and to be following the principles of professional journalism, the evidence presented here argues that all four channels disregarded professional news values. Even the state-run al-Iraqia channel, that is supposed to be independent, showed preferences for some topics over others.
The ‘ancient’ body of modern Yoga: The influence of Ramanuja on Iyengar Yoga and the use of Yoga in actor training
Authors: Kapsali Maria 
Published: October 2013

Abstract
Yoga is one of the most popular leisure activities that flourish in contemporary western societies and also one of the most common non-theatrical disciplines used in performer training. This article focuses on the pedagogy that underlies the practice of yoga and poses and outlines two different ways of practising: the active and passive. It draws links between the pedagogy of modern yoga and the conceptualization of the human body in the pre-modern religious doctrine of Ramanuja. Based on this account, it presents the ways in which yoga can inform the actor’s work with different dramaturgies. In this manner, this article aims to examine modern yoga from a perspective that has been neglected so far and also proposes additional ways in which yoga may be used in actor training.
Quantification of linezolid in serum by LC-MS/MS using semi-automated sample preparation and isotope dilution internal standardization
Authors: Zander Johannes , Maier Barbara , Zoller Michael , Teupser Daniel , Vogeser Michael 
Published: October 2013

Abstract

Background: Linezolid serum concentrations have been shown to be highly variable in critically ill patients with often sub-therapeutic drug levels regarding minimal inhibitory concentrations for relevant pathogens. Consequently, therapeutic drug monitoring of linezolid must be considered, requiring a reliable and convenient analytical method. We therefore developed and validated an LC-MS/MS method applying isotope dilution internal standardization and on-line solid phase extraction for serum linezolid quantification.

Methods: Sample preparation was based on protein precipitation and on-line solid phase extraction with two-dimensional liquid chromatography and column switching. Three-fold deuterated linezolid was used as the internal standard. The method was validated involving two separate LC-MS/MS systems covering the concentration range of 0.13–32 mg/L. The run time was 4 min.

Results: Validation revealed good analytical performance, with inaccuracy <6% and imprecision of <7.3% (CV) for six quality control samples (0.38–16.0 mg/L). The method was found to be robust during the validation process and during a pharmacokinetic study so far involving 600 samples. Comparative measurements on two LC-MS/MS systems revealed close agreement.

Conclusions: This LC-MS/MS assay described herein is a convenient, robust and reliable method for linezolid quantification in serum which can be routinely applied using different LC-MS/MS systems. The method can be used for clinical studies and subsequent TDM of linezolid.
Microstructural Investigation of W-Cu Composite and 1Cr18Ni9 Stainless Steel Brazed Joint with Ag-Cu Filler Metal
Authors: Xia C. Z. , Liang Q. H. , Zou J. S. , Xu X. P. 
Published: October 2013

AbstractAbstract: Tungsten-copper (W-Cu) composite and 1Cr18Ni9 stainless steel were brazed with Ag-28wt%Cu brazing filler metal in a vacuum furnace, via controlling brazing temperature 855–865 °C and holding time 30 min. Microstructure, microhardness, four-point bend strength, element distribution and phase constituents near interface of the brazed joint, were investigated by a series of standard technique. Results indicated that Ag-Cu eutectic phase near W-Cu composite side and the bulk phase near 1Cr18Ni9 steel side were formed in the brazing seam region. Microhardness of the eutectic phase was low, while that of the bulk copper-rich phase was high. Four-point bend strength was as high as 576 MPa, and the fracture occurred at interface near 1Cr18Ni9 steel side. On the brazed interface, Cu and a little W element were transited from W-Cu composite into the brazing seam region, as same as Fe from 1Cr18Ni9 steel. Ag contained in the brazing filler metal was diffused into the W-Cu composite. Element concentration of Ag was high at interface of both two base metals, which was helpful to promote the metallurgical reaction. Phase constituents of the brazed joint were Cu(Ag, Fe), Ag(Cu) and a little of Ag0.7Fe0.3 and FeCu4, formed in the metallurgy of Fe from 1Cr18Ni9 steel and the Ag-Cu brazing filler metal.
Drying Kinetics, Biochemical and Functional Properties of Products in Convective Drying ofAnchovy (Engraulis anchoita) Fillets
Authors: de Moraes Kelly , de Almeida Pinto Luiz Antonio 
Published: October 2013

Abstract
The aim of this work was to study the convective drying of anchovy (Engraulis anchoita) fillets and to evaluate the final product characteristics through its biochemical and functional properties. The drying temperatures were of 50, 60 and 70°C, and the fillet samples were dried with the skins down (with air flow one or the two sides) and skins up (with air flow one side). The drying experimental data were analyzed by Henderson–Pabis model, which showed a good fit (R
2 > 0.99 and REQM < 0.05). The moisture effective diffusivity values ranged from 4.1 × 10–10 to 8.6 × 10–10 m2 s−1 with the skin down and 2.2 × 10–10 to 5.5 × 10–10 m2 s−1 with the skin up, and the activation energy values were 32.2 and 38.4 kJ mol−1, respectively. The product characteristics were significantly affected (p < 0.05) by drying operation conditions. The lower change was in drying at 60°C with air flow for two sides of the samples and skin up. In this condition, the product showed solubility 22.3%; in vitro digestibility 87.4%; contents of available lysine and methionine 7.21 and 2.64 g 100 g−1, respectively; TBA value 1.16 mgMDA kg−1; specific antioxidant activity was 1.91 mMDPPH g−1 min−1, and variation total color was 10.72.
Optical microfibers and nanofibers
Authors: Wu Xiaoqin , Tong Limin 
Published: October 2013

AbstractAs a combination of fiber optics and nanotechnology, optical microfibers and nanofibers (MNFs) have been emerging as a novel platform for exploring fiber-optic technology on the micro/nanoscale. Typically, MNFs taper drawn from glass optical fibers or bulk glasses show excellent surface smoothness, high homogeneity in diameter and integrity, which bestows these tiny optical fibers with low waveguiding losses and outstanding mechanical properties. Benefitting from their wavelength- or sub-wavelength-scale transverse dimensions, waveguiding MNFs exhibit a number of interesting properties, including tight optical confinement, strong evanescent fields, evident surface field enhancement and large and abnormal waveguide dispersion, which makes them ideal nanowaveguides for coherently manipulating light, and connecting fiber optics with near-field optics, nonlinear optics, plasmonics, quantum optics and optomechanics on the wavelength- or sub-wavelength scale. Based on optical MNFs, a variety of technological applications, ranging from passive micro-couplers and resonators, to active devices such as lasers and optical sensors, have been reported in recent years. This review is intended to provide an up-to-date introduction to the fabrication, characterization and applications of optical MNFs, with emphasis on recent progress in our research group. Starting from a brief introduction of fabrication techniques for physical drawing glass MNFs in Section 2, we summarize MNF optics including waveguiding modes, evanescent coupling, and bending loss of MNFs in Section 3. In Section 4, starting from a “MNF tree” that summarizes the applications of MNFs into 5 categories (waveguide & near field optics, nonlinear optics, plasmonics, quantum & atom optics, optomechanics), we go to details of typical technological applications of MNFs, including optical couplers, interferometers, gratings, resonators, lasers and sensors. Finally in Section 5 we present a brief summary of optical MNFs regarding their current challenges and future opportunities.