bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abordaje actualizado del pénfigo foliáceo
Authors: Tobón Arango María Alejandra , Sánchez Pupo María Lucía , Eraso Guerrero Javier Camilo , Paz Chamorro Angela Daniela , Vanegas Martínez Tatiana Paola , Revelo Cháves Hugo Armando 
Published: August 2024

El pénfigo hace referencia a un grupo de enfermedades autoinmunitarias, ampollosas y crónicas de la piel cuya prevalencia descrita es de 0,5 a 3,2 casos por 100.000 habitantes. Dentro de las manifestaciones clínicas que lo destacan se resaltan lesiones localizadas o generalizadas consistentes en ampollas flácidas, frágiles poco aparentes sobre una base eritematosa, la piel parece cubierta por escamas y costras; no afecta las mucosas. El diagnóstico se realiza mediante la evaluaciones clínicas, histológica, detección de autoanticuerpos circulantes dirigidos a antígenos específicos y serológicas, una vez se llega con el diagnóstico el siguiente paso consiste en instaurar un adecuado tratamiento, en caso de esta patología , el objetivo del manejo es suprimir el sistema inmunológico para evitar la formación de nuevas lesiones y curar las ampollas existentes en la piel y/o mucosas, al tiempo que se minimizan los efectos secundarios graves del tratamiento, para cumplir con esto, los corticoides son la primera línea sin embargo, la selección del tratamiento debe ajustarse a la gravedad de la enfermedad, la respuesta a la terapia y las características individuales del paciente. Por ello el presente artículo de revisión, se hablará del abordaje actualizado y alternativas terapéuticas nuevas utilizadas en el pénfigo foliáceo.
Revised status of the genus Tortrilema Volynkin with descriptions of a new genus and a new species (Lepidoptera: Erebidae: Arctiinae: Lithosiini)
Authors: Volynkin Anton V. 
Published: August 2024

The genus-group name Tortrilema Volynkin, 2021 is upgraded to species level and restricted to the ‘postmaculosa Matsumura’ species-group sensu Volynkin (2021) while the ‘labahe Volynkin & Saldaitis species-group’ sensu Volynkin (2021) is erected to the new genus Tortribitecta gen. n. A new species of the genus Tortrilema, Tortrilema fangae sp. n. is described from south-eastern mainland China (Fujian-Jiangxi border). Four new combinations are introduced: Tortrilema postmaculosa (Matsumura, 1927), comb. n., Tortrilema lienquan (Volynkin & Saldaitis, 2021), comb. n., Tortribitecta nubes (Volynkin, 2021), comb. n., and Tortribitecta labahe (Volynkin & Saldaitis, 2021), comb. n. Adults as well as male and female genitalia of both genera along with the related Parabitecta Hering in Draeseke, 1926 are illustrated.
Two new species of the genus Parabitecta Hering in Draeseke from Southwestern China (Lepidoptera: Arctiinae: Lithosiini)
Authors: Volynkin Anton V. , Černý Karel 
Published: August 2024

The footman moth genus Parabitecta Hering in Draeseke, 1926 is re-characterised and compared to the genera Tortrilema Volynkin, 2021 and Tortribitecta Volynkin, 2024. Two new species are described from Southwestern China (Yunnan Province): Parabitecta yanmena sp. n. and Parabitecta baoshana sp. n. Parabitecta secunda Volynkin & Saldaitis, 2020 is reported from Myanmar for the first time. Adults as well as male and female genitalia are illustrated.
eDNAqua-Plan—Standardisation Overview for eDNA Sequencing of Aquatic Organisms and the Downstream Data Ecosystem
Authors: Pauperio Joana , Woollard Peter , Boulanger Emilie , Cochrane Guy , Costa Filipe , Exter Katrina , Grabowski Michal , Kavakiotis Ioannis , Krawczyk Dawid , Laamanen Tiina , Martins Filipa MS , Moutinho Jorge , Papakostas Spiros , Picazo-Moz Antonio , Probert Ian , Rimet Frédéric , Seweryn Michał , Suominen Saara , Pade Nicolas , Consortium eDNAqua-Plan 
Published: August 2024

The eDNAqua-Plan project stands as a beacon of innovation in the biomonitoring of marine and freshwater ecosystems, propelled by the urgent need to integrate DNA-based approaches in aquatic bioassessment and monitoring frameworks. The broad utilisation of cutting-edge environmental DNA (eDNA) and DNA barcoding methodologies is dependent on complete, reliable, and accessible reference DNA sequence data (Rimet et al. 2021). Complete and interoperable metadata is crucial to allow a broad reuse of (e)DNA data and analysis outputs, and for a broader uptake of results by end users.
The eDNAqua-Plan project aims to address key limitations to the routine implementation of eDNA-based monitoring methods in Europe by developing plans for federated DNA barcode reference libraries and eDNA data repositories to support DNA-based environmental monitoring. This will ensure a sustainable and reliable infrastructure to underpin its broad use, thereby paving the way for more effective conservation and management strategies. The project is working towards creating a comprehensive overview of standardisation efforts and data workflows, through collaborations with other projects, initiatives and infrastructures for aquatic monitoring across the European Union (EU) and associated countries. We are analysing existing archives (e.g., International Nucleotide Sequence Database Collaboration (INSDC), Barcode of Life Data System (BOLD), Global Biodiversity Information Facility (GBIF), Ocean Biodiversity Information System (OBIS)), portals, and papers to determine current and best practices through the use of questionnaires, manual evaluation of repositories, and machine learning methods (LLMs). This includes an overview of the usage of existing metadata and data standards (e.g., Minimum Information about any (X) Sequence Specifications from the Genomics Standards Consortium (GSC), Darwin Core standard). The results are being integrated by a team of experts in marine and freshwater biomonitoring.
With a diverse consortium comprising 18 partner institutions from 11 countries and one international institute, eDNAqua-Plan brings together experts in marine and freshwater monitoring, eDNA analysis, and data science. The collective effort by this consortium will lay the groundwork for the creation of a digital ecosystem of eDNA repositories and an integrated reference library of marine and freshwater species, adhering to FAIR (Findable, Accessible, Interoperable, and Reusable) principles.
Green synthesis of silver nanoparticles by cyanobacterial extracts: an approach guarantees potential bioactivity and proper cereal seed germination
Authors: Aly Bahieeldin E. , Mona Badawi H. , Higazy Aziz M. 
Published: August 2024

Background
The last few decades witnessed the adoption of green nanotechnology as an environmentally friendly and cost-effective strategy with several biomedical, industrial and agricultural applications. Here, cyanobacteria have been suggested as model microorganisms for bio-nanoparticles production.

Objective
In the present study, the extracts of a number of cyanobacterial isolates representing different genera and isolated from various aquatic environments of Egypt were explored as a novel source of bioactive silver-based nano-materials.

Materials and methods
The synthesized nanoparticles were characterized by UV-visible spectroscopy (UV-Vis), transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). Results revealed the presence of secondary metabolites in the aqueous extracts necessary for the nano-material biosynthesis. The biological potentials of the produced crude extract-based silver nanoparticles (AgNPs) assessed as antimicrobials indicated high activities against several bacterial and fungal pathogens. These bioactive nanoproducts exhibited antioxidant effects as well.

Results and conclusion
When extract-based AgNPs were tested for seed germination and seedling development of barley (cvs. Giza-123, Giza-2000) and wheat (cvs. Benisweif-7, Misr-3), relative increases in the germination percentages, germination rate index (GRI%), germination velocity coefficient (GVC%) were scored together with somewhat reductions in the mean germination times (MGT). All in all, the findings of this work emphasis that such silver nanoparticles possess antimicrobial and antioxidant activities besides supporting seed germination and seedling development, hence they are highly recommended as an alternative to high-risk chemically synthetic agrochemicals with no expected phytotoxicity.
Immunostimulatory and anti-inflammatory properties of Silene succulenta Forssk
Authors: El Gendy Mohamed A.M. , Motawe Hemaia M. , Maamoun Maii A.I. , El-Akad Radwa H. , Afifi Ahmed H. 
Published: August 2024

Background and objectives
Immunomodulation using medicinal plants is a useful alternative to classical conventional chemotherapy for several diseases, including cancer and autoimmune disorders. Therefore, the aim of this study was to investigate the immunomodulatory properties of Silene succulenta Forssk. extract that is a common herb growing in Egypt.

Materials and methods

S. succulenta was collected from Mediterranean coast region of Egypt, dried in shade, ground, and extracted using methanol. The effect of the plant extract on immune cells was tested using murine splenocytes and human peripheral blood mononuclear cells (PBMCs) proliferation colorimetric assays. A bioassay-directed fractionation was applied to identify the most active fraction and gas chromatography–mass spectrometry (GC–MS) was used to analyze it. The effect of the plant extract and its active fraction on human interleukin-2 (IL-2) was determined using enzyme-linked immunosorbent assay. Furthermore, the effect on macrophage nitric oxide production ability, macrophage viability, and HL-60-differentiated cells to neutrophil ROS production was tested.

Results and conclusion
Our results demonstrated that S. succulenta extract induced murine splenocytes and PBMCs proliferation in a concentration-dependent manner. Through bioassay-directed fractionation, we identified hexane subfraction as the active fraction of the extract and identified 18 compounds using GC–MS with major components of palmitic acid (33.19%) and phthalic acid, di(6-methylhept-2-yl) ester (20.22%). The extract and its active fraction induced human IL-2, whereas they inhibited macrophage nitrite-production ability, and neutrophil reactive oxygen species production level. We concluded that S. succulenta possesses several immunostimulatory and anti-inflammatory properties that can be utilized to treat several diseases.
Effect of licorice root and cabbage leaf extracts as a natural fertilizer on growth and productivity of Cynara cardunculus L
Authors: El-Gohary Ahmed E. , Wahba Hend El-Sayed , Hendawy Saber Fayez , Hussein Mohamed Salah 
Published: August 2024

Background
There is an ongoing need to find safe natural sources of plant nutrients. Licorice root and cabbage leaf extracts are being used as sources that can be used for growth and yield of crops to substitute inorganic fertilizers.

Objective
To study the effect of extracts of cabbage leaves (waste) and licorice roots on Cynara cardunculus L.

Materials and methods
This experiment was carried out during two seasons (2017/2018 and 2018/2019) at Aladlya Field, Sekem, Sharkia Governorate, Egypt, to study the influence of some plant extracts, that is cabbage leaves’ extract at 0, 1, 2, and 3 g/l as well as licorice root’s extract at 0, 5, 10, and 15 g/l, on growth, yield, and chemical constituents (NPK, total phenolic content, and phenolic compounds) of C. cardunculus L. plants.

Results and conclusion
Both licorice root and cabbage leaf extracts had positive effects compared with control. However, licorice root extract had more effect on C. cardunculus L. plants compared with cabbage leaves’ extract. The main phenolic compounds were apigeni-7-glucoside (50.9272–161.8283 μg/g), rutin (79.8306–152.3828 μg/g), chlorogenic (4.5107–25.7202 μg/g).
Influence of date palm pollen grain extract on rabbit buck semen characteristics throughout chilled storage period of 72 h
Authors: El-Seadawy Islam El-Sayed , Kotp Mohamed S. , Hozyen Heba F. , El-Nattat Walid S. , El-Tohamy Magda M. 
Published: August 2024

Background
The demand for using stored semen in artificial insemination programs of livestock animals is increasing. Therefore, developing and improving methods for semen preservation would provide adequate fertility rates that maintain the high production rates for rabbit industry. Several studies on preservation protocols and extender composition have been carried out.

Objective
The current study was designed to examine the effect of various concentrations of date palm pollen grain extract (DPPE) on postchilling quality of rabbit semen.

Materials and methods
Total phenolic and flavonoid substances and antioxidant activity were assessed in DPPE. High-performance liquid chromatography was used for identification and separation of goal metabolites. Semen was gathered from 10 male rabbits, grouped, and then split into five fractions (500 μl each). The first fraction represented as control, whereas DPPE was supplemented at concentrations of 1.6, 2.0, 2.4, and 2.8 mg/5 ml tris-citric extender. Extended semen specimens were cooled at 4°C for 72 h. Motile, life, abnormal, membrane, and acrosome integrity percentages of sperms were appraised in chilled semen all over the refrigeration period.

Results and conclusion
Total phenolic and total flavonoids contents in the DPPE were 4.15 mg GAE/g extract and 0.74 mg CE/g extract, respectively. The DPPE specimen showed various antiradical activity gauged toward 2,2-azino-bis/3-ethil-benothiazoline-6-sulfonic acid (12.37 mM TE/g) and 2,2-Diphenyl-1-picryl-hydrazyl (4.06 mM TE/g). However, the reducing capacity assessed by ferric reducing activity power method was 9.19 mM TE/g/g. The most effective compounds in the DPPE were pyrogallol (4150.92 μg/g extract), ferulic acid (2935.50 μg/g extract), and rutin (2163.99 μg/g extract). The enrichment of semen extenders with 2.4 mg DPPE/5 ml tris-citric extender had preserved the sperm forward motility, sperm livability, sperm acrosome integrities, and sperm membrane integrities in an upright state during cooling till 72 h related to control treating. No adverse effects were recorded on sperm abnormalities. It could be concluded that the enriching of rabbit bucks’ semen tris-basic extender by 2.4 mg DPPE/5 ml tris-extender (the perfect and harmless concentration) sustained the sperm features in decent conditions all over a cooling period of 72 h.
Effect of algae and yeast on the production of essential oil and some active constituents in rosemary
Authors: Fouad Rasha , Fouad Hend , Aziz Eman E. , Nofal Osama A. , Rezk Abdelhalim I. , El-Nasharty Adel B. , Omer Elsayed A. 
Published: August 2024

Background
Rosemary is an important medicinal plant and one of the main aromatic spices in the world. Nowadays, it is very important to use natural substances such as algae and yeast in the green agriculture to increase quantity and quality of crops, in addition to preserving environment from the harms of using chemicals in the agriculture.

Objective
The study aimed to investigate growth, yield, and active constituents of rosemary under foliar spraying of different concentrations of both algae and yeast extracts.

Materials and methods
The experiment was performed during the two successive seasons 2019 and 2020 in completely randomized blocks design and consisted of seven treatments: two biostimulants with three levels of each factor, in addition to the control (tap water). Algae extract was sprayed with concentrations of 0.5, 1, and 2 g/l, whereas yeast concentrations were 5, 10, and 20 g/l. The growth parameters, total phenolics, antioxidant activity, essential oil percentage, yield, and its main constituents were studied.

Results and conclusion
The main components of essential oil were found to be endo-borneol followed by (+)-2-bornanone. The growth, yield, total phenolics, antioxidant activity, essential oil, and the main components of rosemary increased with all used concentrations of algae and yeast extracts compared with control. These increments reached their maximum with application of algae at 1 and 2 g/l and yeast extract at 10 and 20 g/l. In general, spraying yeast extract resulted in the highest average of growth, yield, and chemical constituents of rosemary, and the best parameters were obtained by spraying yeast at 20 g/l. It is recommended to spray rosemary with yeast extract at a dose of 20 g/l to obtain the best plant herbal yield, essential oil, total phenolic content, and antioxidant activity.
Exogenous utilization of jasmonic acid and methyl jasmonate stimulates growth and biochemical composition of lavender (Lavandula angustifolia) plant
Authors: El-Ziat Rasha A. , Soliman Dina M. , El-Sayed Iman M. 
Published: August 2024

Background
Lavender (Lavandula angustifolia) as an economic herb is used in traditional medicine, perfume, flavoring, and cosmetics. It is classified in the mint family (Lamiaceae) and commonly used as a landscape plant. Monoterpenes and sesquiterpenes are the most common chemical components found in essential oils. Because of these compounds, it is used for antifungal, antibacterial, and antioxidant activities. Secondary metabolite compounds, that is, jasmonic acid (JA) and methyl jasmonate (MeJA), significantly influence secondary metabolism regulation by stimulating the accumulation of phenols, flavonoids, and alkaloids.

Objective
The research aimed to estimate the effect of both JA and MeJA on growth parameters, chemical composition, particularly secondary metabolism, and the composition of volatile oils of L. angustifolia Mill plants.

Materials and methods
In the experiment, JA concentrations of 0, 1, 5, and 10 mM and MeJA concentrations of 0, 0.25, 0.50, and 1.0 mM were used as foliar spray applications on L. angustifolia. The investigation was performed as a randomized complete block design in three replicates. The parameters collected were different growth parameters, essential oil components using gas chromatography–mass spectrometry, the number of glands and secretory trichome diameter by scanning electron microscope, as well as chemical constituents.

Results and conclusion
The results showed that growth parameters were increased gradually with the increasing of JA and MeJA concentrations. The effects of JA and MeJA at high concentrations were more effective on all the growth parameters, biochemical components, and the number of glands and diameter of secretory trichomes than other concentrations and control plants in both seasons. JA application significantly increased chlorophyll a and b, carotenoids, and N and protein content of lavender plants. MeJA gave the highest values of total sugar, free amino acids, and phenols. Therefore, the results suggested that 10 mM of JA and 1.0 mM of MeJA significantly stimulate lavender plant growth and chemical compounds and volatile oil components, especially linalool and camphor, increasing its value and raising its quality in the perfume and cosmetics industries and various chemical industry applications.
Synthesis, characterization, anticancer activity, and molecular docking of novel maleimide–succinimide derivatives
Authors: Mutlaq Dakhil Z. , Al-Shawi Ali A.A. , Al-Asadi Rafid H. 
Published: August 2024

Background and objective
A wide range of maleimide heterobifunctional reagents are used for the preparation of targeted therapeutics. Succinimide derivatives are important compounds found in a variety of natural products that exhibit remarkable biological and pharmaceutical activity. The creation of new maleimide–succinimide derivatives will increase the importance and medicinal applications of these groups.

Materials and methods
The reaction of bismaleimide (1–2) with phenylhydrazide and 4-methylbenzohydrazide resulted in the formation of N’-[1-(4-[2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl] phenyl)-2,5-dioxopyrrolidin-3-yl] benzohydrazide (3), N’-[1-(4-[2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl] phenyl)-2,5-dioxopyrrolidin-3-yl]-4-methylbenzohydrazide (4), N’-[1-(4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-[1,1’-biphenyl]-4-yl)-2,5-dioxopyrrolidin-3-yl] benzohydrazide (5), and N’-[1-(4-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)-[1,1’- biphenyl]-4-yl)-2,5-dioxopyrrolidin-3-yl]-4-methylbenzohydrazide (6). The interaction of potential compounds with AKT1 and CDK2 proteins was performed using molecular docking to target the hydrogen bond and amino acid residues.

Results
The new compounds were characterized using Fourier-transform infrared spectroscopy,1H-NMR,13C-NMR spectroscopy, and mass spectrometry. The MTT assay was used to test cell viability against breast cancer cells (MCF-7). The cytotoxicity results revealed that compounds 3 and 5 were more toxic than compounds 4 and 6. Molecular docking of compounds that interacted with AKT1 and CDK2 showed affinity energy of −16.112 and −21.342 kcal/mol for compound 3, while −22.398 and −19.940 kcal/mol for compound 5. The root-mean-square deviation values for CDK2 and AKT1 were 2.27 and 1.61 for compound 3, respectively, and 1.93 and 1.90 for compound 5.

Conclusion
Toxicity and molecular docking studies revealed that compounds 3 and 5 could be developed as anticancer agents against breast cancer, indicating that further research is warranted.
Evaluation of inflammatory markers in egyptian obese and nonobese adolescents
Authors: El-Shaheed Azza Abd , Fahmy Reham F. , Mahfouz Nermine N. , Elabd Mona A. , Sibaii Hiba , El-Zayat Salwa Refat 
Published: August 2024

Background
Obesity causes an inflammation state that is related to co-morbidities such as hypertension and insulin resistance. Though, the underlying inflammatory events in obesity are still obscure particularly in pediatric age group.

Objective
To identify the initial stage of inflammation linked to this nutritional disorder.

Materials and methods
A case control observational study involved 45 cases of overweight/obese adolescents and 44 healthy peers of matching age and sex. Serum levels of Interleukin-10, Interleukin-18, and C − reactive protein were estimated. Leukocytes, Neutrophils, Lymphocytes counts were determined, and Neutrophil/ Lymphocyte Ratio was calculated.

Results and conclusion
C − reactive protein, Neutrophils, and Neutrophil/Lymphocyte Ratio were significantly higher in overweight/ obese adolescents (P = 0.000, P = 0.015, and P = 0.034, respectively). Interleukin-10 was non-significantly lower whereas IL-18 was non-significantly higher in overweight/ obese adolescents (P = 0.334, and P = 0.427, respectively). Obesity is accompanied by a low inflammatory state that exists since childhood. Therefore, timely interventions should be adopted to preclude the deleterious consequences in grown up adults.