bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genomic Resources Notes accepted 1 February 2015 – 31 March 2015
Authors:   Genomic Resources Development Consortium, Arthofer Wolfgang , Bertini Laura , Caruso Carla , Cicconardi Francesco , Delph Lynda F. , Fields Peter D. , Ikeda Minoru , Minegishi Yuki , Proietti Silvia , Ritthammer Heike , Schlick‐Steiner Birgit C. , Steiner Florian M. , Wachter Gregor A. , Wagner Herbert C. , Weingartner Laura A. 
Published: June 2015

AbstractThis article documents the public availability of (i) raw transcriptome sequence data, assembled contigs and BLAST hits of the Antarctic plant Colobanthus quitensis grown in two different climatic conditions, (ii) the draft genome sequence data (raw reads, assembled contigs and unassembled reads) and RAD‐tag read data of the marbled flounder Pseudopleuronectes yokohamae, (iii) transcriptome resources from four white campion (Silene latifolia) individuals from two morphologically divergent populations and (iv) nuclear DNA markers from 454 sequencing of reduced representation libraries (RRL) based on amplified fragment length polymorphism (AFLP) PCR products of four species of ants in the genus Tetramorium.
Multi‐slice computed tomography characteristics of solitary pulmonary ground‐glass nodules: Differences between malignant and benign
Authors: Hu Haiyang , Wang Qingguo , Tang Huamei , Xiong Liwen , Lin Qiang 
Published: June 2015

AbstractBackgroundGround‐glass nodules (GGNs), which are possible precursors of lung cancer, attract increasing attention. Many studies have attempted to identify the characteristic imaging features of GGNs for their qualitative diagnosis; however, the comprehension of GGNs remains controversial. We performed this study to identify imaging characteristics helpful to the differential diagnosis of solitary GGNs.MethodsWe retrospectively evaluated 112 solitary GGNs resected from 112 patients, pathologically examined after surgical resection. Imaging features of the GGNs, such as size, shape, a solid component, lobulation, spiculation, vascular convergence sign, pleural tag, and air cavity density, were assessed. Differences between malignant and benign nodules were analyzed using binary logistic regression analysis.ResultsOf the 112 GGNs, 82 were malignant and 30 were benign. A solid component, vascular convergence sign, and a larger diameter were risk factors for malignancy, with a sensitivity, specificity, and accuracy of 93.9%, 60.0%, and 84.8%, respectively. Lobulation, spiculation, air cavity densities, and pleural tags were also important indicators of malignancy, with positive predictive values of 93.5%, 83.3%, 91.7%, and 87.2%, respectively.ConclusionGGNs with a solid component, vascular convergence sign, and a larger diameter are highly suggestive of malignancy. The possibility of a neoplasm should also be considered in the case of GGNs that show lobulation, spiculation, air cavity densities, or pleural tags. To obtain a comprehensive and accurate analysis of the nodules, three‐dimensional reconstruction is highly recommended.
Beta‐globin gene evolution in the ruminants: evidence for an ancient origin of sheep haplotype B
Authors: Jiang Y. , Wang X. , Kijas J. W. , Dalrymple B. P. 
Published: June 2015

SummaryDomestic sheep (Ovis aries) can be divided into two groups with significantly different responses to hypoxic environments, determined by two allelic beta‐globin haplotypes. Haplotype A is very similar to the goat beta‐globin locus, whereas haplotype B has a deletion spanning four globin genes, including beta‐C globin, which encodes a globin with high oxygen affinity. We surveyed the beta‐globin locus using resequencing data from 70 domestic sheep from 42 worldwide breeds and three Ovis canadensis and two Ovis dalli individuals. Haplotype B has an allele frequency of 71.4% in O. aries and was homozygous (BB) in all five wild sheep. This shared ancestry indicates haplotype B is at least 2–3 million years old. Approximately 40 kb of the sequence flanking the ~37‐kb haplotype B deletion had unexpectedly low identity between haplotypes A and B. Phylogenetic analysis showed that the divergent region of sheep haplotype B is remarkably distinct from the beta‐globin loci in goat and cattle but still groups with the Ruminantia. We hypothesize that this divergent ~40‐kb region in haplotype B may be from an unknown ancestral ruminant and was maintained in the lineage to O. aries, but not other Bovidae, evolving independently of haplotype A. Alternatively, the ~40‐kb sequence in haplotype B was more recently acquired by an ancestor of sheep from an unknown non‐Bovidae ruminant, replacing part of haplotype A. Haplotype B has a lower nucleotide diversity than does haplotype A, suggesting a recent bottleneck, whereas the higher frequency of haplotype B suggests a subsequent spread through the global population of O. aries.
Relationship between wild greylag and European domestic geese based on mitochondrial DNA
Authors: Heikkinen M. E. , Ruokonen M. , Alexander M. , Aspi J. , Pyhäjärvi T. , Searle J. B. 
Published: June 2015

SummaryThe origins of the European domestic goose are uncertain. The available information comes from archaeological findings and historical literature, but genetic evidence has hitherto been scarce. The domestic goose in Europe is derived from the greylag goose (Anser anser), but it is not known where the initial domestication took place and which of the two subspecies of greylag goose was ancestral. We aimed to determine the amount and geographical distribution of genetic diversity in modern populations of greylag geese as well as in different breeds of the domestic goose to make inferences about goose domestication. We studied DNA sequence variation in the mitochondrial control region of greylag geese from multiple populations across Europe and western Asia as well as specimens of domestic geese representing 18 modern breeds and individuals not belonging to any recognised breed. Our results show notable differences in genetic diversity between different greylag goose populations and the presence of six mitochondrial haplogroups which show a degree of geographical partitioning. The genetic diversity of the domestic goose is low, with 84% of sampled individuals having one of two major closely related haplotypes, suggesting that modern European domestic geese may derive from a narrow genetic base. The site of domestication remains unresolved, but domestic geese in Turkey were unusually diverse, indicating the importance of further sampling in the vicinity of the eastern Mediterranean and the Near East. There appears to be past or ongoing hybridisation between greylags and domestic geese in particular areas, consistent with field observations.
Prevalence of Deafness‐Associated Connexin‐26 (GJB2) and Connexin‐30 (GJB6) Pathogenic Alleles in a Large Patient Cohort from Eastern Sicily
Authors: Amorini Maria , Romeo Petronilla , Bruno Rocco , Galletti Francesco , Di Bella Chiara , Longo Patrizia , Briuglia Silvana , Salpietro Carmelo , Rigoli Luciana 
Published: June 2015

SummaryMutations in the gene encoding the gap junction protein connexin 26 (GJB2) and connexin 30 (GJB6) have been shown to be a major contributor to prelingual, sensorineural, nonsyndromic deafness.The aim of this study was to characterize and establish the prevalence of GJB2 and GJB6 gene alterations in 196 patients affected by sensorineural, nonsyndromic hearing loss, from Eastern Sicily. We performed sequence analysis of GJB2 and identified sequence variants in 68 out of 196 patients (34.7%); (28 homozygous for c.35delG, 22 compound heterozygous and 11 with only one variant allele). We found 12 different allelic variants, the most prevalent being c.35delG, which was found on 89 chromosomes (65.5%), followed by other alleles with different frequencies (p.E47X, c.‐23+1G>A, p.L90P, p.R184W, p.M34T, c.167delT, p.R127H, p.M163V, p.V153I, p.W24X, and p.T8M).Importantly, for the first time we present the frequency and spectrum of GJB2 mutations in NSHL patients from Eastern Sicily. No alterations were found in the GJB6 gene, confirming that alterations in this gene are uncommon in our geographic area.Note that 65.3% and 23.5% of our patients, respectively were found to be negative or carriers by GJB2 molecular screening. This emphasizes the need to broaden the genetic analysis to other genes involved in hearing loss.
Common Regulatory Variants ofCYFIP1Contribute to Susceptibility for Autism Spectrum Disorder (ASD) and Classical Autism
Authors: Wang Jian , Tao Yu , Song Fan , Sun Yue , Ott Jurg , Saffen David 
Published: June 2015

SummaryBased on the analysis of mRNA expression and genotype data from the “Brain Cloud” database, we identified seven SNPs within or near the autism candidate geneCYFIP1that show nominally significant correlations between genotype andCYFIP1mRNA expression in human dorsolateral prefrontal cortex. Analysis of transmission disequilibrium test (TDT) odds ratios (ORs) for these SNPs in a large Autism Genome Project (AGP) trio‐based association study revealed the high‐expression alleles of four of these SNPs (rs8028440, rs2289823, rs7403800 and rs3751566) to be susceptibility alleles. Correlations between the regression coefficients for mRNA expression and log10‐transformed TDT ORs were statistically significant [P = 0.008 (ASD); P = 0.002 (classical autism)]. Similarly, statistically significant correlations were obtained between levels ofCYFIP1mRNA expression predicted using the regression equations obtained from multiple linear regression analysis and log10‐transformed TDT ORs for specific combinations of genotypes for both ASD (rs2289823 + rs3751566: P = 0.008) and classical autism (rs2289823 + rs3751566: P = 0.008; rs2289823 + rs3751566 + rs765763: P = 0.0006) diagnoses. Together, these results support the hypothesis that high expression ofCYFIP1mRNA increases susceptibility for both ASD and classical autism.
Unmethylated‐maspin DNA in maternal plasma is associated with severe preeclampsia
Authors: Qi Yan‐Hua , Teng Fei , Zhou Qi , Liu Yu‐Xin , Wu Jin‐Fang , Yu Shan‐Shan , Zhang Xin , Ma Miao‐Yan , Zhou Ni , Chen Li‐Juan 
Published: June 2015

AbstractIntroductionCell‐free fetal DNA in maternal plasma is associated with complications of pregnancy, including preeclampsia. Determination of levels is affected by fetal gender and genetic polymorphisms. Unmethylated maspin (u‐maspin) is present in the placenta, and is placental‐specific. The purpose of this study was to determine whether u‐maspin DNA in maternal blood could serve as a marker of preeclampsia by measuring levels in different trimesters of normal pregnancies and in those complicated by preeclampsia.Material and methodsThis case‐control study was set in a tertiary care hospital.The population consisted of 45 women with normal pregnancies (15 in the 1st trimester, 15 in the 2nd trimester, 15 in the 3rd trimester), 20 women with mild preeclampsia, 25 women with severe preeclampsia, and six women with gestational trophoblastic disease.Peripheral blood was collected and methylation‐specific PCR and fluorescence quantitative PCR were performed to measure the content of u‐maspin DNA in maternal blood.ResultsU‐maspin DNA was 5.5‐fold higher in women with severe preeclampsia than in those with a normal 3rd trimester pregnancy (p < 0.05). During normal pregnancy, u‐maspin DNA in maternal plasma tended to increase with advancing gestational age (p = 0.06). U‐maspin DNA was not detected in healthy non‐pregnant women or those with gestational trophoblastic disease.ConclusionU‐maspin DNA in maternal blood is associated with severe preeclampsia.
A surgical technique using the ovarian vein in non‐human primate models of potential living‐donor surgery of uterus transplantation
Authors: Kisu Iori , Banno Kouji , Mihara Makoto , Hara Hisako , Umene Kiyoko , Adachi Masataka , Nogami Yuya , Aoki Daisuke 
Published: June 2015

AbstractAimLiving donor surgery in organ transplantation should be performed in a minimally invasive manner under conditions that are as safe as possible. The objective of this study is to examine whether the procedure for using the ovarian vein makes donor surgery less invasive in a cynomolgus monkey model of potential living‐donor surgery of uterus transplantation.Material and methodsTwenty‐two female cynomolgus monkeys aged 6–9 years and with body weights of 3.55 ± 1.28 kg were used in the study. Vessels and tissues surrounding the uterus were dissected while preserving the uterine artery/vein. The deep uterine vein was used as a venous pedicle in four monkeys (Group 1), and the ovarian vein was used instead of the deep uterine vein in 18 monkeys (Group 2). With the uterine artery/vein and deep uterine vein (Group 1) or ovarian vein (Group 2) connected to the uterus, the vaginal canal was cut. The vessels were then clamped to produce a donor surgery model. Surgical time, intraoperative organ and vascular injury were examined in each animal.ResultsThe average surgical time from laparotomy to clamping of vessels was 230 ± 112 min in all 22 cynomolgus monkeys, and significantly longer in Group 1 (n = 4) than in Group 2 (n = 18) (393 ± 71 vs. 194 ± 84 min, p < 0.05). Surgical time in Group 2 showed a tendency to decrease in animals treated later in the study, with a significantly longer time in the first 10 monkeys compared with the last 8 (253 ± 65 vs. 120 ± 26 min, p < 0.05). All monkeys had no complications, including no injuries to other organs and no unanticipated vascular injury.ConclusionThe procedure using the ovarian vein was less invasive than that using the deep uterine vein in mimicking living‐donor surgery in a cynomolgus monkey model of uterus transplantation.
Preterm birth may be a larger risk factor for increased blood pressure than intrauterine growth restriction
Authors: Steen Emma , Bonamy Anna‐Karin , Norman Mikael , Hellström‐Westas Lena 
Published: June 2015

AbstractAimVery low birthweight (VLBW) and prematurity have been associated with an increased risk of high blood pressure (BP). We compared BP and salivary cortisol responses to a stressful situation between adolescents with a VLBW and controls.MethodsWe compared three groups aged 12–17 years: 30 born VLBW but appropriate for gestational age (AGA) at a mean of 27 weeks, 19 born VLBW but small for gestational age (SGA) at a mean of 31 weeks and 43 term‐born AGA controls. Three consecutive BP measurements were performed before a magnetic resonance imaging (MRI) examination. Salivary cortisol and perceived stress were assessed before and after the MRI.ResultsSystolic and diastolic BP decreased significantly for each repeated measurement in the VLBW‐SGA group and controls, but remained unchanged in the VLBW‐AGA group. The third systolic BP measurement was 9–12 mmHg higher in the VLBW‐AGA group than the other groups (p < 0.05). There were no differences in salivary cortisol between the groups, before and after the MRI or between the sexes.ConclusionDynamic BP responses differed between adolescents born VLBW‐AGA and the other groups, indicating that extremely preterm birth may be a larger risk factor for increased BP than intrauterine growth restriction.
Adverse Events Associated with Flumazenil Treatment for the Management of Suspected Benzodiazepine Intoxication – A Systematic Review with Meta‐Analyses of Randomised Trials
Authors: Penninga Elisabeth I , Graudal Niels , Ladekarl Morten Bækbo , Jürgens Gesche 
Published: June 2015

AbstractFlumazenil is used for the reversal of benzodiazepine overdose. Serious adverse events (SAEs) including seizures and cardiac arrhythmias have been reported in patients treated with flumazenil, and the clinical advantage of flumazenil treatment has been questioned. The objective was to assess the risk of (S)AEs associated with the use of flumazenil in patients with impaired consciousness due to known or suspected benzodiazepine overdose. Studies included in the meta‐analyses were identified by literature search in Medline, Cochrane Library and Embase using combinations of the words flumazenil, benzodiazepines, anti‐anxiety agents, poisoning, toxicity and overdose. Randomised clinical trials (RCTs) in verified or suspected benzodiazepine overdose patients comparing treatment with flumazenil versus placebo were included. Pre‐defined outcome measures were AEs, SAEs and mortality. Thirteen trials with a total of 994 randomised (990 evaluable) patients were included. AEs were significantly more common in the flumazenil group (138/498) compared with the placebo group (47/492) (risk ratio: 2.85; 95% confidence interval: 2.11–3.84; p < 0.00001). SAEs were also significantly more common in the flumazenil group compared with the placebo group (12/498 versus 2/492; risk ratio: 3.81; 95% CI: 1.28–11.39; p = 0.02). The most common AEs in the flumazenil group were agitation and gastrointestinal symptoms, whereas the most common SAEs were supraventricular arrhythmia and convulsions. No patients died during the blinded phase of the RCTs. The use of flumazenil in a population admitted at the emergency department with known or suspected benzodiazepine intoxication is associated with a significantly increased risk of (S)AEs compared with placebo. Flumazenil should not be used routinely, and the harms and benefits should be considered carefully in every patient.
Depression and prospection
Authors: Roepke Ann Marie , Seligman Martin E. P. 
Published: June 2015

ObjectivesProspection, the mental representation of possible futures, is usually adaptive. When it goes awry, however, it disrupts emotion and motivation. A negative view of the future is typically seen as one symptom of depression, but we suggest that such negative prospection is the core causal element of depression. Here, we describe the empirical evidence supporting this framework, and we explore the implications for clinical interventions.MethodsWe integrate several literatures: Using the database PsycInfo, we retrieved empirical studies with the keywords prospection, prediction, expectation, pessimism, mental simulation, future‐thinking, future‐directed thinking, foresight, and/or mental time travel, in conjunction with depression, depressed, or depressive.ResultsThree kinds of faulty prospection, taken together, could drive depression: Poor generation of possible futures, poor evaluation of possible futures, and negative beliefs about the future. Depressed mood and poor functioning, in turn, may maintain faulty prospection and feed a vicious cycle. Future‐oriented treatment strategies drawn from cognitive‐behavioural therapy help to fix poor prospection, and they deserve to be developed further.ConclusionsProspection‐based techniques may lead to transdiagnostic treatment strategies for depression and other disorders.Practitioner points

Faulty prospection may be the core process underlying depression.

Three general problems of prospection, taken together, could drive depression: Poor generation of possible futures, poor evaluation of possible futures, and negative beliefs about the future.

Faulty prospection can be helped using future‐oriented treatment strategies from cognitive‐behavioural therapy, and basic research on prospection points to additional future‐oriented clinical strategies for alleviating depression.

More research is needed to determine whether prospection drives depression, and whether future‐focused interventions are more effective than those focused on the past and present.
Bullying: The impact of teacher management and trait emotional intelligence
Authors: Casas José A. , Ortega‐Ruiz Rosario , Del Rey Rosario 
Published: June 2015

BackgroundThe bullying phenomenon has serious consequences for those that are involved. In order to find more effective ways to eradicate it from the schools, more research is needed. In this context, teacher management and emotional intelligence (EI) are shown to be relevant keys to consider.AimThe aim of this study was to analyse the ways in which teacher management and trait EI affect involvement in bullying aggression and victimization.SampleA total of 2,806 Spanish schoolchildren (51.8% girls; Age M = 15.44; SD = 1.79) participated in this transversal study.MethodsSelf‐report questionnaires were administrated; four of the dimensions of the Schoolwide Climate Scale: Bullying victimization; bullying aggression; positive teacher management; and negative teacher management. They also completed the Spanish version of the TMMS‐24 EI questionnaire. After encoding the data, six structural equation models were created to study the direct and joint effects of teacher management and trait EI on bullying aggression and victimization. The models were run for both the whole sample and split samples based on the education cycles and sex.ResultsResults showed that both positive and negative teacher management were closely linked to involvement in bullying aggression and victimization. EI was also found to be directly related to bullying involvement. Furthermore, results revealed that teacher management was directly related to trait EI. Education cycle differences were found, but no sex‐specific differences were apparent in the sample.ConclusionsConclusions of the study highlight the important role that teacher management plays with regard to bullying involvement and trait EI, and include a discussion of the need to include teacher management in bullying prevention programmes at schools.
Using virtual online simulations in Second Life® to engage undergraduate psychology students with employability issues
Authors: Ward Tony , Falconer Liz , Frutos‐Perez Manuel , Williams Bryn , Johns James , Harold Sinead 
Published: June 2015

AbstractThis study compares online simulation in Second Life® (Linden Labs, San Francisco, California, USA) with equivalent face‐to‐face activities for three scenarios. The intention was that the three sets of activities would increase participant awareness of how psychology is applied in relation to work‐based contexts. These were a Dragons' Den‐style activity to increase awareness of entrepreneurialism, a supermarket‐based activity based on consumer and work psychology and a counselling agency. After engaging in the activities, participants completed various measures, including a satisfaction questionnaire. In the supermarket scenario, Second Life® was rated significantly better in terms of student satisfaction and the extent to which awareness of the application of psychology in this context had increased. For the other scenarios, Second Life® and face‐to‐face activities were largely equivalent on the various measures. The exception was that in the online counselling scenario, participants did not indicate to a significant degree that they were now more aware of how psychology was applied in this setting. This might be linked to the perceived quality of interaction with the chatbot avatars. It is suggested that the overall superiority of the online supermarket scenario is because this complex problem‐based activity achieved greater immersion in the online version.A video abstract of this article can be viewed at https://youtu.be/_NrZiElz_UA.