bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of Eight Periodontal Microorganisms and Distribution of Porphyromonas gingivalis fimA Genotypes in Chinese Patients With Aggressive Periodontitis
Authors: Feng Xianghui , Zhang Li , Xu Li , Meng Huanxin , Lu Ruifang , Chen Zhibin , Shi Dong , Wang Xiane 
Published: May 2013

Background: The microbiologic feature of aggressive periodontitis (AgP) in Chinese patients has not yet been determined. This study aims to investigate the prevalence of eight periodontal microorganisms and the distribution of the Porphyromonas gingivalis fimA genotype in a cohort of Chinese patients with AgP.Methods: Saliva and pooled subgingival plaque samples were collected from 81 patients with AgP (25 with incisor–first molar type and 56 with generalized type [GAgP]) and 34 periodontally healthy controls. Eight periodontal microorganisms, including Aggregatibacter actinomycetemcomitans, P. gingivalis, Tannerella forsythia, Treponema denticola, Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, and Fusobacterium nucleatum were detected in these samples by the polymerase chain reaction (PCR). In addition, the distribution of fimA genotypes was assessed in P. gingivalis–positive individuals by PCR.Results: The prevalence of P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, F. nucleatum, and A. actinomycetemcomitans in patients with AgP was significantly higher than that in healthy controls. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low (30.4%) compared with other pathogens. Results of logistic regression analysis showed that younger patients were more likely to harbor A. actinomycetemcomitans (odds ratio = 2.85). Type II was the most prevalent fimA genotype of P. gingivalis in patients with AgP.Conclusions: P. gingivalis, T. forsythia, T. denticola, C. rectus, P. intermedia, and F. nucleatum were the predominant periodontal pathogens of patients with GAgP in China. Type II of fimA was the most prevalent genotype of P. gingivalis in patients with AgP. The prevalence of A. actinomycetemcomitans in patients with GAgP was relatively low.
The forgotten goiter: casuistic contribution and considerations for the choice of surgical approach
Authors: Avenia Nicola , Santoprete Stefano , Monacelli Massimo , Lucchini Roberta , Cirocchi Roberto , Sanguinetti Alessandro , Triola Roberta , Vannucci Jacopo , Corsi Alessia , Avenia Stefano , Puma Francesco 
Published: May 2013

Abstract
Aim. A residual mediastinal thyroid (“forgotten goiter”) is a well-known, though uncommon, complication of total thyroidectomy. Materials of study. The authors analyze their experience with three cases of goiter forgotten, observed in a series of 2946 thyroid resections in the period 2005–2010. In the study, a preoperative CT of the chest with three-dimensional reconstruction was always performed to examine the topographical relationships of the lesion. Excision was performed through cervicotomy, cervicosternotomic approach and cervicosternotomy, and posterolateral right thoracotomy. Results. There were no complications. Histological examination was suggestive of malignancy in one case (follicular carcinoma with pulmonary metastases). Discussion. The indication for surgery in cases of forgotten goiter is intrinsic to the diagnosis. Preoperative evaluation with accurate topographic imaging is required in all cases in order to understand the nature and location of mediastinal pathological tissue and to identify the most suitable access route. The cervicotomy is the ideal access for low surgical trauma and is easily extendable into a partial or complete sternotomy. A thoracotomy, on the other hand, which is usually reserved for the right side, must be planned preoperatively.
Thyroiditis de Quervain
Authors: Klett R. , Braun S. , Zimny M. , Schenke S. 
Published: May 2013

SummaryBackground: Subacute thyroiditis is a usually self-limiting disease of the thyroid. However, approximately 0.5–15% of the patients require permanent thyroxine substitution. Aim was to determine predictive factors for the necessity of long-term hormone-replacement (LTH). Patients, methods: We retrospectively reviewed the records of 72 patients with subacute thyroiditis. Morphological and serological parameters as well as type of therapy were tested as predictive factors of consecutive hypothyroidism. Results: Mean age was 49 ± 11 years, f/m-ratio was 4.5 : 1. Thyroid pain and signs of hyperthyroidism were leading symptoms. Initial subclinical or overt hyperthyroidism was found in 20% and 37%, respectively. Within six months after onset 15% and 1.3% of the patients developed subclinical or overt hypothyroidism, respectively. At latest follow-up 26% were classified as liable to LTH. At onset the thyroid was enlarged in 64%, and at latest follow-up in 8.3%, with a significant reduction of the thyroid volume after three months. At the endpoint the thyroid volume was less in patients in the LTH group compared with the non-LTH group (41.7% vs. 57.2% of sex-adjusted upper norm, p = 0.041). Characteristic ultrasonographic features occurred in 74% of the patients in both lobes. Serological and morphological parameters as well as type of therapy were not related with the need of LTH. Conclusions: In this study the proportion of patients who received LTH was 26%. At the endpoint these patients had a lower thyroid volume compared with euthyroid patients. No predictive factors for LTH were found.
Genetic diversity of androdioecious Osmanthus fragrans (Oleaceae) cultivars using microsatellite markers
Authors: Duan Yifan , Wang Xianrong , Xiang Qibai , Liang Lili , Li Xuexia , Liu Yulian , Li Meng 
Published: May 2013

• Premise of the study: For cultivar classification, identification, and genetic improvement, microsatellite markers were developed to analyze the genetic diversity of androdioecious Osmanthus fragrans cultivars.• Methods and Results: Fifteen microsatellite markers were developed from sequences downloaded from the National Center for Biotechnology Information, which included two with null alleles. These primers were screened on 62 typical androdioecious O. fragrans cultivars belonging to four groups (Asiaticus, Albus, Luteus, and Aurantiacus). The number of alleles ranged from two to six, with a mean of 3.7 per locus. The observed and expected heterozygosities ranged from 0.1000 to 0.9091 and from 0.1287 to 0.9167, respectively. Results from structure analyses indicated that Asiaticus and Albus were genetically mixed, and Luteus and Aurantiacus were partially genetically differentiated.• Conclusions: These markers will be useful for genetic study of androdioecious O. fragrans cultivars and facilitate cultivar classification, particularly for the cultivar groups Luteus and Aurantiacus.
Development of microsatellite markers for the neotropical vine Dalechampia scandens (Euphorbiaceae)
Authors: Falahati‐Anbaran Mohsen , Stenøien Hans K. , Pélabon Christophe , Bolstad Geir H. , Perez‐Barrales Rocio , Hansen Thomas F. , Armbruster W. Scott 
Published: May 2013

• Premise of the study: Microsatellite markers were developed to assess polymorphism and level of genetic diversity in four Mexican populations of the neotropical vine Dalechampia scandens (Euphorbiaceae).• Methods and Results: Thirty‐seven microsatellite markers representing bi‐, tri‐, tetra‐, and pentanucleotide microsatellite repeats were developed. In total, 166 alleles were identified across 54 individuals. The number of alleles varied from one to 11 with an average of 4.49 alleles per locus. All loci except one were highly polymorphic between populations, whereas considerably less variation was detected within populations for most loci. The average observed and expected heterozygosities across study populations ranged from 0 to 0.63 and 0 to 0.59, respectively, for individual loci, and a deviation from Hardy–Weinberg equilibrium was observed for most loci.• Conclusions: The developed markers may be useful for studying genetic structure, parentage analysis, mapping, phylogeography, and cross‐amplification in other closely related species of Dalechampia.
An improved clearing and mounting solution to replace chloral hydrate in microscopic applications
Authors: Villani Thomas S. , Koroch Adolfina R. , Simon James E. 
Published: May 2013

• Premise of the study: This study presents Visikol™, a new proprietary formulation that can be used as an efficient replacement for chloral hydrate as a clearing agent for microscopic examination. In the United States, chloral hydrate is regulated and therefore difficult to acquire.• Methods and Results: Fresh and dry samples of the following plants: ginger (Zingiber officinale), maté (Ilex paraguariensis), lime basil (Ocimum americanum), oregano (Origanum vulgare), and mouse‐ear cress (Arabidopsis thaliana), were cleared using Visikol or chloral hydrate solution and compared using a light microscope.• Conclusions: This new method can be used successfully to clear specimens, allowing identification of diagnostic characteristics for the identification of plant materials. Visikol is as effective as chloral hydrate in providing clarity and resolution of all tissues examined. Tissues become transparent, allowing observation of deeper layers of cells and making it effective in research, botanical and quality control, and for educational applications.
La mobilité dans la "ville durable" : la construction de l’évidence du Tramway par des dynamiques transactionnelles
Authors: Hamman Philippe 
Published: May 2013

L’article interroge le développement durable urbain en France à partir des politiques de déplacements, en retenant l’exemple du tramway dans deux grandes agglomérations : Montpellier et Strasbourg. La dimension transversale de ces projets urbains est analysée sous l’angle de la diversité de leurs acteurs et de leurs processus, participant de la fabrique de la ville, plutôt que rapportée uniquement à la question des transports. Cette approche relationnelle permet de penser ensemble la ville et l’environnement comme les deux pôles d’un même enjeu, l’opérationnalisation de la « ville durable » ; ses traductions concrètes passent par un ensemble de transactions sociales débouchant sur des compromis pratiques que les projets de tramways rendent particulièrement visibles.
Élaboration d’un protocole de suivi de la fréquentation au sein de la Réserve naturelle marine de la Réunion, France, Océan Indien
Authors: Lemahieu Anne , Pennober Gwenaëlle , David Gilbert , Lavigne Franck , Pothin Karine , Gérard Léa 
Published: May 2013

Créée en 2007, La Réserve Naturelle Marine de La Réunion (RNMR) vise à préserver un récif frangeant qui s’étend sur près d’une quarantaine de kilomètres de linéaire côtier sur la façade occidentale de l’île. Rendue fortement attractive par ses nombreux atouts balnéaires, cette côte a été en proie à une urbanisation croissante au cours des dernières décennies. De façon concomitante, l’accroissement de la fréquentation et l’apparition de nouvelles pratiques nautiques ont alimenté la source potentielle d’impacts sur le milieu, ainsi que les risques de conflits. En vue d’apporter un meilleur éclairage sur ces problématiques et parce que la mesure de l’efficacité d’une AMP ne peut désormais plus se cantonner aux seuls indicateurs biologiques du milieu, une vaste campagne nationale visant à développer un cadre méthodologique commun de mesure de la fréquentation maritime a été initiée (Agence des Aires Marines Protégées). C’est dans ce contexte et par le biais de cette étude que la RNMR se pourvoit dès 2010 d’un cadre méthodologique et opérationnel de suivi de la fréquentation afin de développer et d’intégrer à leurs plans de gestion des indicateurs socio-économiques qui serviront, entre autres, à mesurer l’efficacité de la gestion. Entre janvier et juillet 2010, pas moins de 10 catégories d’usages dont les activités de baignade, plage, sports de glisse et pêche ont fait l’objet d’un suivi depuis un Ultra-Léger-Motorisé et à l’aide d’un appareil photographique. 48 vols ont été répartis sur cette période selon une stratification temporelle pré-établie (Été/Hiver, Vacances/Périodes scolaires, semaine/week-end et matin/Après-midi). Les données ont été intégrées à une Base d’Information Géographique offrant un arsenal d’outils d’analyse spatiale et statistique. Les premiers résultats font état d’une « fréquentation différentielle » dans le temps et l’espace, dont les pics peuvent atteindre près de 10 000 usagers. La moitié des usagers reste concentrée sur une faible portion du linéaire côtier équivalente à 8 % de la longueur totale de la réserve et se répartit sur 4 principaux sites (Boucan-Canot, l’Ermitage, la Saline et Etang-Salé) qui s’avèrent être les plus pourvus en aménités (dépressions d’arrière-récif, sable corallien) et en aménagements (stationnements, bars, maîtres-nageurs sauveteurs). En outre, le protocole de suivi de la fréquentation de la RNMR présente de nombreuses potentialités en termes de gestion et de capitalisation scientifique, lesquelles sont présentées en discussion.
La consommation énergétique à Calcutta (Inde) : du confort thermique aux statuts sociaux
Authors: Pellegrino Margot 
Published: May 2013

Cet article analyse une pratique de consommation énergétique à forte retombée : l’utilisation croissante de l’air conditionné à l’intérieur des espaces publics et privés indiens. Pour répondre à la question « La climatisation est-elle nécessaire ? », nous avons étudié, à partir d’un travail de terrain à Calcutta, le lien entre mesure technique et perception subjective du confort thermique, et entre consommation énergétique et pratiques sociales, en s’appuyant sur une approche méthodologique à la fois sociale et ingénieurale. L’étude a permis de montrer comment une donnée physique objective, dans ce cas la température, renvoie à des perceptions subjectives variables. L’étude montre de la même façon que le rapport entre perception subjective (confort thermique) et pratique énergétique (utilisation des climatiseurs) n’est pas linéaire. Les résultats de cette étude permettent finalement de comprendre que les comportements énergétiques d’une partie de l’échantillon enquêté sont déterminés par une aspiration sociale, qui vise à démontrer, à travers la possession d’un objet (le climatiseur) et la modalité de son utilisation (indépendante d’une nécessité réelle et perçue), l’appartenance à un statut social précis et identifiable. Du coup, la définition du confort thermique elle-même ne peut pas se passer de l’analyse sociale : dans le cas étudié, elle ne dépend pas seulement d’une réponse physique subjective à une donnée environnementale, mais elle relève également des représentations propres à chacune des catégories sociales.
Inhaled Carbon Monoxide Accelerates Resolution of Inflammation via Unique Proresolving Mediator–Heme Oxygenase-1 Circuits
Authors: Chiang Nan , Shinohara Masakazu , Dalli Jesmond , Mirakaj Valbona , Kibi Megumi , Choi Augustine M. K. , Serhan Charles N. 
Published: May 2013

Abstract
Resolution of acute inflammation is an active event accompanied by biosynthesis of specialized proresolving mediators (SPM). We employed a systems approach to determine the impact of CO in resolution active programs during self-limited inflammation in mice. Compared with ambient air, inhaled CO gas (250 ppm) significantly limited PMN infiltration (∼44%, 6 h) into peritoneum and shortened resolution interval from 4 to 2 h. We profiled exudate lipid mediators (LM) via metabololipidomics, CO reduced leukotriene B4 (21 ± 11 versus 59 ± 24 pg/mouse, 6 h), and elevated SPM including resolvin (Rv) D1 (27 ± 4 versus 16 ± 5 pg/mouse) and maresin 1 (26 ± 9 versus 15 ± 3 pg/mouse). With human macrophages, SPM (10 pM–10 nM) elevated heme oxygenase (HO)-1 (∼50%, 8 h). CO also enhanced HO-1 expression and accumulation of RvD1 and RvD5, an action reversed by blockage of a key SPM biosynthesis enzyme 15-lipoxygenase type 1. Compared with normoxia, CO increased ∼30% phagocytosis of opsonized zymosan with human macrophage, which was further enhanced by SPM (∼100%). This CO increased phagocytosis was blocked by 15-lipoxygenase inhibition, and SPM stimulated phagocytosis was diminished by HO-1 inhibition. In murine peritonitis, both pre- and posttreatment with CO inhalation significantly increased macrophages carrying ingested apoptotic PMN in exudates and enhanced PMN apoptosis. Taken together, these results indicate that CO accelerates resolution of acute inflammation, shortens resolution intervals, enhances macrophage efferocytosis, and temporally regulates local levels of lipid mediator/SPM. Moreover, they provide proresolving mechanisms for HO-1/CO, which is part of the SPM-initiated resolution circuit.
Roles of Adipocytes and Fibroblasts in Activation of the Alternative Pathway of Complement in Inflammatory Arthritis in Mice
Authors: Arend William P. , Mehta Gaurav , Antonioli Alexandra H. , Takahashi Minoru , Takahashi Kazue , Stahl Gregory L. , Holers V. Michael , Banda Nirmal K. 
Published: May 2013

Abstract
The complement system is involved in mediation of joint damage in rheumatoid arthritis, with evidence suggesting activation of both the classical and alternative pathway (AP). The AP is both necessary and sufficient to mediate collagen Ab–induced arthritis, an experimental animal model of immune complex–induced joint disease. The AP in mice is dependent on MASP-1/3 cleavage of pro–factor D (pro-FD) into mature factor D (FD). The objectives of the current study were to determine the cells synthesizing MASP-1/3 and pro-FD in synovial tissue. Collagen Ab–induced arthritis was studied in wild-type C57BL/6 mice, and the localization of mRNA and protein for FD and MASP-1/3 in synovial adipose tissue (SAT) and fibroblast-like synoviocytes (FLS) was determined using various techniques, including laser capture microdissection. SAT was the sole source of mRNA for pro-FD. Cultured differentiated 3T3 adipocytes, a surrogate for SAT, produced pro-FD but no mature FD. FLS were the main source of MASP-1/3 mRNA and protein. Using cartilage microparticles (CMPs) coated with anti-collagen mAb and serum from MASP-1/3−/− mice as a source of factor B, pro-FD in 3T3 supernatants was cleaved into mature FD by MASP-1/3 in FLS supernatants. The mature FD was eluted from the CMP, and was not present in the supernatants from the incubation with CMP, indicating that cleavage of pro-FD into mature FD by MASP-1 occurred on the CMP. These results demonstrate that pathogenic activation of the AP can occur in the joint through immune complexes adherent to cartilage and the local production of necessary AP proteins by adipocytes and FLS.
Combined thrombin-collagen injection for the management of an iatrogenic pseudoanuerysm in the popliteal region
Authors: McCarthy Eoghan , O′Mahony Niamh , Ryan Mark , Guiney Michael 
Published: May 2013

AbstractPseudoaneurysm formation is a rare but recognized complication of total knee arthroplasty (TKR), with fewer than 20 cases described in the literature. Multiple management techniques have been described for such pseudoaneurysms. As thrombin injection is an established technique, we report a case of a post-TKR tibioperoneal pseudoaneurysm successfully occluded via percutaneous injection of a dual thrombin-collagen preparation.
Etiology and significance of incidentally detected focal colonic uptake on FDG PET/CT
Authors: Purandare Nilendu C , Gawade Sachin K , Puranik Ameya D , Agrawal Archi , Shah Sneha , Rangarajan Venkatesh 
Published: May 2013

Abstract
Background: Incidental colonic uptake of 18F-flurodeoxyglucose (FDG) is not an infrequent finding encountered during whole body positron emission tomography (PET) imaging. Almost all studies on this topic are in Western populations, which have a markedly different epidemiological profile for colorectal premalignant and malignant conditions as compared to that of the Indian subcontinent. Aim: The purpose of this study was to assess the etiology of incidentally detected focal FDG uptake in the colon by comparing it with colonoscopy and histopathology. Materials and Methods: Electronic medical records of patients who underwent FDG PET/computed tomography (CT) at our institution for a 2½-year period from January 2009 to July 2011 were reviewed. There were 32 out of 9000 (0.35%) patients whose PET/CT reports mentioned incidental focal colonic FDG uptake, of which 24 patients subsequently underwent colonoscopy. Lesions which appeared neoplastic on colonoscopy were confirmed with histopathology obtained after biopsy or surgery. Colonoscopy and pathology findings were considered as gold standard. Results: Among the 24 patients who underwent a colonoscopy, 3 patients had normal findings (12.5%). A positive colonoscopy was noted in 21 patients (87.5%) with the lesion coinciding with the location described in the PET/CT report. Adenomatous polyps were detected in 12 patients (37.5%), whereas in 8 patients (25%) malignant lesions were confirmed [adenocarcinoma n = 5, non-Hodgkin′s lymphoma (NHL) n = 2, malignant melanoma n = 1]. In one patient, colonic uptake was diagnosed as inflammatory. The mean standardized uptake value max (SUV max ) for the 12 premalignant lesions was 16.9 ± 9.6 (range 7.5-37.4) and the mean SUV max for the 8 malignant lesions was 12.9 ± 5.5 (range 6.7-21.6). The difference in SUV max between the premalignant adenomatous polyps and the malignant lesions was not statistically significant (P = 0.316). Conclusions: Our study shows that a significant proportion of patients (62.5%, 20/32) showing an incidental focal FDG uptake will harbor premalignant (adenomatous polyps) or malignant lesions, and further evaluation with colonoscopy and biopsy is warranted in such cases.
Case Series: Pericardial gossypibomas detected after cardiovascular surgery: Imaging findings
Authors: Erol Cengiz , Koplay Mustafa , Paksoy Yahya , Kanat Fikret 
Published: May 2013

AbstractWe present the chest radiograph, computed tomography (CT), and magnetic resonance imaging (MRI) findings of three pericardial gossypibomas, which are rarely reported and an exceptional complication of cardiovascular surgery. The diagnosis is usually possible with surgical history, high clinical suspicion, and awareness of variable imaging findings. Usage of sponges with radiopaque markers facilitates early detection by chest radiographs and CT. In case of radiolucent gossypibomas, specific MRI features help to differentiate this pathology from other masses and diffusion-weighted images can be used to distinguish it from an abscess.