bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating metabolic profiling of pancreatic juice with transcriptomic analysis of pancreatic cancer tissue identifies distinct clinical subgroups
Authors: Pulvirenti Alessandra , Barbagallo Marialuisa , Putignano Anna Rita , Pea Antonio , Polidori Rebecca , Upstill-Goddard Rosie , Cortese Nina , Kunderfranco Paolo , Brunelli Laura , De Simone Giulia , Pastorelli Roberta , Spaggiari Paola , Nappo Gennaro , Jamieson Nigel B. , Zerbi Alessandro , Chang David K. , Capretti Giovanni , Marchesi Federica 
Published: June 2024

IntroductionMetabolic reprogramming is a hallmark feature of pancreatic ductal adenocarcinoma (PDAC). A pancreatic juice (PJ) metabolic signature has been reported to be prognostic of oncological outcome for PDAC. Integration of PJ profiling with transcriptomic and spatial characterization of the tumor microenvironment would help in identifying PDACs with peculiar vulnerabilities.MethodsWe performed a transcriptomic analysis of 26 PDAC samples grouped into 3 metabolic clusters (M_CL) according to their PJ metabolic profile. We analyzed molecular subtypes and transcriptional differences. Validation was performed by multidimensional imaging on tumor slides.ResultsPancreatic juice metabolic profiling was associated with PDAC transcriptomic molecular subtypes (p=0.004). Tumors identified as M_CL1 exhibited a non-squamous molecular phenotype and demonstrated longer survival. Enrichment analysis revealed the upregulation of immune genes and pathways in M_CL1 samples compared to M_CL2, the group with worse prognosis, a difference confirmed by immunofluorescence on tissue slides. Enrichment analysis of 39 immune signatures by xCell confirmed decreased immune signatures in M_CL2 compared to M_CL1 and allowed a stratification of patients associated with longer survival.DiscussionPJ metabolic fingerprints reflect PDAC molecular subtypes and the immune microenvironment, confirming PJ as a promising source of biomarkers for personalized therapy.
Investigating the relationship between postoperative radiotherapy and intestinal flora in rectal cancer patients: a study on efficacy and radiation enteritis
Authors: Long Lin , Zhang Yexi , Zang Jianhua , Liu Peng , Liu Wei , Sun Cheng , Tian Dan , Li Ping , Tian Jin , Xiao Jun 
Published: June 2024

ObjectiveThis study aimed to investigate the impact of radiation therapy and radiation enteritis on intestinal flora, providing insights for treatment and prevention.MethodsFecal samples were collected from 16 patients undergoing pelvic radiotherapy at Qingdao Hiser Hospital Affiliated of Qingdao University (Qingdao Traditional Chinese Medicine Hospital). Samples were collected before and after radiotherapy (27–30Gy), and analyzed using DNA sequencing and biostatistical methods.ResultsPatients with radiation enteritis showed increased α-diversity and β-diversity of intestinal flora compared to those without radiation enteritis. Differences in flora composition were observed, with higher abundance of secondary pathways such as amino acid metabolism, carbohydrate metabolism, cofactors and vitamins metabolism, and lipid metabolism.ConclusionThe study revealed that patients developing radiation enteritis during pelvic radiation therapy had increased diversity and abundance of intestinal flora compared to those who did not develop radiation enteritis. Additionally, patients without radiation enteritis showed significantly higher diversity and abundance of intestinal flora post-radiation compared to pre-radiation.
Pharmacokinetic profile of phenytoin in dried blood spot with high-performance liquid chromatography—photodiode array
Authors: Harahap Yahdiana , Ng Limeylia , Sunarsih Sunarsih 
Published: June 2024

Phenytoin is a first-line antiepileptic drug with narrow therapeutic range and follows non-linear pharmacokinetics. Pharmacokinetics of phenytoin have been studied in plasma matrix before, however, there were several disadvantages. This study aimed to obtain partial validation data of the analytical method and the pharmacokinetic profile of phenytoin in Dried Blood Spot (DBS) of six healthy subjects. DBS has the advantage of only requiring small sample volumes and could be transported more efficiently. Phenytoin along with carbamazepine as the chosen internal standard was analyzed with a reversed-phase high performance-liquid chromatography system and a photodiode array detector at 205 nm. The results of partial validation, which evaluated the linearity, within-run accuracy, and precision, were within the criteria acceptance range. The pharmacokinetic profile showed that average AUC0-t was 83.81 ± 37.32 μg.h/mL and AUC0-∞ was 83.65 ± 38.89 μg.h/mL with an average ratio of 93%. Previous study quantifying phenytoin in the plasma matrix found the average AUC0-t was 39.41 ± 8.57 µg.h/mL and AUC0-∞ was 42.94 ± 9.55 µg.h/mL. Despite the difference between parameters of phenytoin analyzed in DBS and plasma matrices, the pharmacokinetic profiles obtained from both matrices were similar indicated by comparable concentration-time curves, thus, proving that DBS matrix can be used interchangeably with the plasma matrix as a more comfortable and effective alternative to phenytoin quantification in blood.
High-intensity interval training using electrical stimulation ameliorates muscle fatigue in chronic kidney disease-related cachexia by restoring mitochondrial respiratory dysfunction
Authors: Fusagawa Hiroyori , Sato Tatsuya , Yamada Takashi , Naito Azuma , Tokuda Nao , Yamauchi Nao , Ichise Nobutoshi , Ogawa Toshifumi , Karaushi Takuro , Teramoto Atsushi , Tohse Noritsugu 
Published: June 2024

BackgroundExercise, especially high-intensity interval training (HIIT), can increase mitochondrial respiratory capacity and enhance muscular endurance, but its systemic burden makes it difficult to safely and continuously prescribe for patients with chronic kidney disease (CKD)-related cachexia who are in poor general condition. In this study, we examined whether HIIT using electrical stimulation (ES), which does not require whole-body exercise, improves muscle endurance in the skeletal muscle of 5/6 nephrectomized rats, a widely used animal model for CKD-related cachexia.MethodsMale Wistar rats (10 weeks old) were randomly assigned to a group of sham-operated (Sham) rats and a group of 5/6 nephrectomy (Nx) rats. HIIT was performed on plantar flexor muscles in vivo with supramaximal ES every other day for 4 weeks to assess muscle endurance, myosin heavy-chain isoforms, and mitochondrial respiratory function in Nx rats. A single session was also performed to identify upstream signaling pathways altered by HIIT using ES.ResultsIn the non-trained plantar flexor muscles from Nx rats, the muscle endurance was significantly lower than that in plantar flexor muscles from Sham rats. The proportion of myosin heavy chain IIa/x, mitochondrial content, mitochondrial respiratory capacity, and formation of mitochondrial respiratory supercomplexes in the plantaris muscle were also significantly decreased in the non-trained plantar flexor muscles from Nx rats than compared to those in plantar flexor muscles from Sham rats. Treatment with HIIT using ES for Nx rats significantly improved these molecular and functional changes to the same degrees as those in Sham rats. Furthermore, a single session of HIIT with ES significantly increased the phosphorylation levels of AMP-activated protein kinase (AMPK) and p38 mitogen-activated protein kinase (MAPK), pathways that are essential for mitochondrial activation signaling by exercise, in the plantar muscles of both Nx and Sham rats.ConclusionThe findings suggest that HIIT using ES ameliorates muscle fatigue in Nx rats via restoration of mitochondrial respiratory dysfunction with activation of AMPK and p38 MAPK signaling. Our ES-based HIIT protocol can be performed without placing a burden on the whole body and be a promising intervention that is implemented even in conditions of reduced general performance status such as CKD-related cachexia.
Determination of genomic regions associated with early storage root formation and bulking in cassava
Authors: Abah Simon Peter , Mbe Joseph Okpani , Dzidzienyo Daniel Kwadjo , Njoku Damian , Onyeka Joseph , Danquah Eric Yirenkyi , Offei Samuel Kwane , Kulakow Peter , Egesi Chiedozie Ngozi 
Published: June 2024

Early cassava storage root formation and bulking is a medium of escape that farmers and processors tend to adopt in cases of abiotic and biotic stresses like drought, flood, and destruction by domestic animals. In this study, 220 cassava genotypes from the International Institute of Tropical Agriculture (IITA), National Root Crops Research Institute (NRCRI), International Center for Tropical Agriculture (CIAT), local farmers (from farmer’s field), and NextGen project were evaluated in three locations (Umudike, Benue, and Ikenne). The trials were laid out using a split plot in a randomized incomplete block design (alpha lattice) with two replications in 2 years. The storage roots for each plant genotype were sampled or harvested at 3, 6, 9, and 12 month after planting (MAP). All data collected were analyzed using the R-statistical package. The result showed moderate to high heritability among the traits, and there were significant differences (p&lt; 0.05) among the performances of the genotypes. The genome-wide association mapping using the BLINK model detected 45 single-nucleotide polymorphism (SNP) markers significantly associated with the four early storage root bulking and formation traits on Chromosomes 1, 2, 3, 4, 5, 6, 8, 9, 10, 13, 14, 17, and 18. A total of 199 putative candidate genes were found to be directly linked to early storage root bulking and formation. The functions of these candidate genes were further characterized to regulate i) phytohormone biosynthesis, ii) cellular growth and development, and iii) biosynthesis of secondary metabolites for accumulation of starch and defense. Genome-wide association study (GWAS) also revealed the presence of four pleiotropic SNPs, which control starch content, dry matter content, dry yield, and bulking and formation index. The information on the GWAS could be used to develop improved cassava cultivars by breeders. Five genotypes (W940006, NR090146, TMS982123, TMS13F1060P0014, and NR010161) were selected as the best early storage root bulking and formation genotypes across the plant age. These selected cultivars should be used as sources of early storage root bulking and formation in future breeding programs.
Mental health index of the elderly population in Medellín (Colombia)−2021: a factorial analysis
Authors: Ochoa-Nieto Sandra Patricia , Benjumea-Gómez Luisa María , Cardona-Arango Doris , Segura-Cardona Ángela , Segura-Cardona Alejandra , Robledo-Marín Carlos 
Published: June 2024

IntroductionThere are several risk factors associated with developing mental disorders among people over 60 years of age. Mental health encompasses multiple domains or capacities, which can comprise the psychological wellbeing of older people. Therefore, resilient coping, self-compassion, self-esteem, hopelessness, quality of life, and depression are considered the characteristics and adaptive mechanisms that bring together the main risk and protective factors for imbalance in mental wellbeing.ObjectiveThis study aimed to establish the demographic, social, and family factors, as well as the clinical characteristics and lifestyle habits that influence the mental health of the elderly people in the city of Medellín, in the year 2021, to build a mental health index.MethodologyThis study adopts a quantitative approach, employs retrospective temporality, and utilizes secondary sources. A cross-sectional survey was carried out with the SABAM Project (health and mental wellbeing of older adults from five cities in Colombia in 2021 by its Spanish acronym) database (secondary source), which is part of the research group “Public Health Observatory” of CES University (Medellín, Colombia). The database comprised 500 records of people aged over 60 years from the city of Medellín in 2021. While constructing the index, a principal component analysis was used, along with the Varimax method for factor analysis rotation.ResultsThe median age of the general population was 67 years (IQR 63–72); for men, the median age was 66 years (IQR 63–71) and for women, the median age was 67 years (IQR 63–72). An association was found between low socioeconomic levels, alcohol consumption, and the level of higher education (university—postgraduate) with low levels of the Mental Health Index in people over 60 years of age in Medellín during the year 2021.ConclusionA quantitative model was developed to predict either a positive or negative prognosis in the mental wellbeing of the population over 60 years of age in the city of Medellín. The model was also used for the development of a sociodemographic profile highlighting the impact on mental health among people over 60 years of age with limited economic resources.
The impact of parenting style on malevolent creativity based on Chinese university students: a latent profile analysis
Authors: Cui Xingnan , Zhang Xiang , Zhang Hua 
Published: June 2024

IntroductionThis study investigated the association between parenting styles and malevolent creativity.MethodsIt used latent profile analysis to compare the differences in malevolent creativity between different combinations of parenting styles with an online sample (N = 620).ResultsThe results of the study suggest that a three-profile solution best fits the data, and the three profiles were labelled positive open parenting, undifferentiated parenting and negative limited parenting. Subsequent analyses revealed that there were significant differences in malevolent creativity performance among the three parenting styles, with participants in the positive open parenting having more malevolent creativity. Those with undifferentiated parenting had the lowest scores.DiscussionThe findings provide theoretical guidance for parenting strategies. Future intervention studies on malevolent creativity should also consider the potential impact of parenting style to obtain better results.
Detection of cerebrospinal fluid biomarkers changes of Alzheimer’s disease using a cognitive stress test in persons with subjective cognitive decline and mild cognitive impairment
Authors: Valles-Salgado Maria , Gil-Moreno María José , Curiel Cid Rosie E. , Delgado-Álvarez Alfonso , Ortega-Madueño Isabel , Delgado-Alonso Cristina , Palacios-Sarmiento Marta , López-Carbonero Juan I. , Cárdenas María Cruz , Matías-Guiu Jorge , Díez-Cirarda María , Loewenstein David A. , Matias-Guiu Jordi A. 
Published: June 2024

IntroductionTimely and accurate diagnosis of the earliest manifestations of Alzheimer’s disease (AD) is critically important. Cognitive challenge tests such as the Loewenstein Acevedo Scales for Semantic Interference and Learning (LASSI-L) have shown favorable diagnostic properties in a number of previous investigations using amyloid or FDG PET. However, no studies have examined LASSI-L performance against cerebrospinal fluid biomarkers of AD, which can be affected before the distribution of fibrillar amyloid and other changes that can be observed in brain neuroimaging. Therefore, we aimed to evaluate the relationship between LASSI-L scores and CSF biomarkers and the capacity of the cognitive challenge test to detect the presence of amyloid and tau deposition in patients with subjective cognitive decline and amnestic mild cognitive impairment (MCI).MethodsOne hundred and seventy-nine patients consulting for memory loss without functional impairment were enrolled. Patients were examined using comprehensive neuropsychological assessment, the LASSI-L, and cerebrospinal fluid (CSF) biomarkers (Aβ1-42/Aβ1-40 and ptau181). Means comparisons, correlations, effect sizes, and ROC curves were calculated.ResultsLASSI-L scores were significantly associated with CSF biomarkers Aβ1-42/Aβ1-40 in patients diagnosed with MCI and subjective cognitive decline, especially those scores evaluating the capacity to recover from proactive semantic interference effects and delayed recall. A logistic regression model for the entire sample including LASSI-L and age showed an accuracy of 0.749 and an area under the curve of 0.785 to detect abnormal amyloid deposition.ConclusionOur study supports the biological validity of the LASSI-L and its semantic interference paradigm in the context of the early stages of AD. These findings emphasize the utility and the convenience of including sensitive cognitive challenge tests in the assessment of patients with suspicion of early stages of AD.
Differentiating the relationships between traditional and new media use and sleep quality during the COVID-19 pandemic: roles of psychological distress and age
Authors: Xu Tong , Zhu Dongmei , Yu Zhaoliang , Dang Junhua , Schiöth Helgi 
Published: June 2024

BackgroundNumerous studies have consistently demonstrated a decline in sleep quality during the COVID-19 pandemic. The primary objective of this study is to explore the impact of engaging with pertinent epidemic information through the media amid the COVID-19 crisis on individuals’ sleep quality and the underlying mechanisms through which this influence operates.MethodsAn online cross-sectional study design was employed. A total of 1,063 British adults (36.2% males; Mage = 38.85, SDage = 13.36, ranging from 18 to 77 years old) participated in the study and completed our questionnaires, which included media usage frequency during the pandemic, the 10-item Kessler Psychological Distress Scale (K10), the Insomnia Severity Index (ISI), and the Ten-item Personality Inventory (TIPI).ResultsPearson’s correlation analyses indicated that there was no significant correlation between COVID-19-related traditional media use (television, radio, newspaper) and psychological distress or sleep quality. However, exposure to information related to COVID-19 through new media use (Facebook, Tik Tok, Twitter) was correlated with greater psychological distress and poorer sleep quality. A moderated mediation analysis showed that psychological distress fully mediated the relationship between new media use and poor sleep, which was moderated by age, with the association between psychological distress and poor sleep quality being stronger among older adults.ConclusionExposure to information of COVID-19 via new (but not traditional) media use deteriorated sleep quality through greater psychological distress, and this relationship was stronger among older adults.
The role of psychological resilience and aggression in injury prevention among martial arts athletes
Authors: Patenteu Ionuț , Gawrych Roman , Bratu Mircea , Vasile Luciela , Makarowski Ryszard , Bitang Andrei , Nica Sarah Adriana 
Published: June 2024

IntroductionFor martial artists, the ability to manage reactions in the face of adversity and bounce back after a stressful event can have major impact on performance. The scope of the research is to investigate martial artists’ level of resilience and aggression (Go-ahead, Foul play, and Assertiveness factors), what is specific to athletes and who have suffered from moderate and/or severe injuries (in terms of resilience and three factors of aggression examined), and test the possibility that a psychological variable under investigation can predict athletes’ injury severity.Materials and methodsA total sample of 154 athletes from striking combat sports—SC (karate, taekwondo, kickboxing, and boxing), grappling combat sports—GC (judo and BJJ), and mixed martial artists (MMA) participated in the research. For assessing resilience, the Romanian adaptation of the Brief Resilience Scale (BRS) was used, and for aggression, the Romanian adaptation of Makarowski’s Sports Aggression Questionnaire was used. An injury report form was also created and applied to athletes.ResultsThe post-hoc tests (after running a single-factor multivariate analysis of variance) revealed significant differences for resilience and Foul (violent) play between the sports disciplines analyzed. A significant positive correlation was found between athletes’ injury severity and assertiveness in SC and between injury severity and resilience in GC. Through the t-test for independent samples, it was highlighted that the average value for Foul (violent) play is significantly higher in athletes who have suffered mild, moderate, and/or severe injuries compared with martial arts athletes who have suffered from only mild/minor injuries. A binomial logistic regression was also performed to verify to what extent Foul play predicts athletes’ injury severity.ConclusionA low level of Foul (violent) play is linked with a decreased likelihood of moderate and/or severe injuries in martial arts athletes. The study findings suggest that resilience, foul play, and assertiveness have an important role in injury prevention among martial artists.
Measuring mental wellbeing in clinical and non-clinical adolescents using the COMPAS-W Wellbeing Scale
Authors: Lam Janine R. , Park Haeme R. P. , Gatt Justine M. 
Published: June 2024

IntroductionAdolescence is a key period of vulnerability for poor mental health as the brain is still developing and may be more sensitive to the negative impacts of stress and adversity. Unfortunately, few measures comprehensively assess wellbeing in adolescents.MethodsThe 26-item COMPAS-W Wellbeing Scale for adults was validated in a sample of 1,078 adolescents aged 13–17 years old (51.67% male, 79.13% non-clinical vs 20.87% psychiatric or developmental clinical cases). The six COMPAS-W sub-scales and total scale were examined in this sample using second-order confirmatory factor analysis, and psychometric testing.ResultsThe 23-item COMPAS-W demonstrated the best fit for this sample according to goodness-of-fit indices (χ2 (220, 1078) = 1439.395, p &lt; 0.001, CFI = 0.893, TLI = 0.877, RMSEA = 0.070, SRMR = 0.095). Internal reliability for the confirmed 23-item COMPAS-W model was run for the total scale (α = 0.912) and sub-scales (Composure, α = 0.735; Own-worth, α = 0.601; Mastery, α = 0.757; Positivity, α = 0.721; Achievement, α = 0.827; and Satisfaction, α = 0.867). Test-retest reliability over 6 weeks was also good for the total scale at r = 0.845 and the sub-scales: Composure (r = 0.754), Own-worth (r = 0.743), Mastery (r = 0.715), Positivity (r = 0.750), Achievement (r = 0.750), and Satisfaction (r = 0.812). Compared with non-clinical participants’ wellbeing (M = 90.375, SE = 0.400), those with clinical diagnoses reported lower wellbeing, both for those with developmental diagnoses (M = 85.088, SE = 1.188), or psychiatric diagnoses (M = 78.189, SE = 1.758), or combined developmental and psychiatric diagnoses (M = 77.079, SE = 2.116). Yet, when wellbeing category scores were considered by diagnosis group, both non-clinical and clinical groups demonstrated incidence across all three categories of languishing, moderate and flourishing wellbeing, in support of the dual-continua model of mental health. On average, younger adolescents’ (13–14 years) wellbeing did not differ from older adolescents’ (15–17 years) wellbeing; however, for sex, males scored 1.731 points significantly higher in wellbeing compared with females (p = 0.028); and American participants scored 3.042 points significantly higher in wellbeing compared with Australian participants (p &lt; 0.001).DiscussionIn conclusion, the 23-item COMPAS-W is a reliable measure of wellbeing for adolescents, both for those with and without developmental and psychiatric diagnoses.