bluuebunny/crossref_metadata_2025_split huggingface dataset:
Barriers to uptake and use of pre-exposure prophylaxis (PrEP) among communities most affected by HIV in the UK: findings from a qualitative study in Scotland
Authors: Young Ingrid , Flowers Paul , McDaid Lisa M 
Published: November 2014

ObjectivesTo explore the acceptability of pre-exposure prophylaxis (PrEP) among gay, bisexual and men who have sex with men (MSM) and migrant African communities in Scotland, UK.DesignConsecutive mixed qualitative methods consisting of focus groups (FGs) and in-depth interviews (IDIs) explored PrEP acceptability. Data were digitally recorded, transcribed and analysed thematically to identify anticipated and emerging themes.SettingParticipants were recruited through community sexual health and outreach support services, and from non-sexual health settings across Scotland.ParticipantsInclusion criteria included identification as either MSM and/or from migrant African communities; 18 years and older; living in Scotland at the time of participation. 7 FGs were conducted (n=33): 5 with MSM (n=22) and 2 mixed-sex groups with African participants (n=11, women=8), aged 18–75 years. 34 IDIs were conducted with MSM (n=20) and African participants (n=14, women=10), aged 19–60 years. The sample included participants who were HIV-positive and HIV-negative or untested (HIV-positive FG participants, n=22; HIV-positive IDI participants, n=17).ResultsUnderstandings of PrEP effectiveness and concerns about maintaining regular adherence were identified as barriers to potential PrEP uptake and use. Low perception of HIV risk due to existing risk management strategies meant few participants saw themselves as PrEP candidates. Participants identified risk of other sexually transmitted infections and pregnancy as a concern which PrEP did not address for either themselves or their sexual partners. PrEP emerged as a contentious issue because of the potentially negative implications it had for HIV prevention. Many participants viewed PrEP as problematic because they perceived thatotherswould stop using condoms if PrEP was to become available.ConclusionsPrEP implementation needs to identify appropriate communication methods in the context of diverse HIV literacy; address risk-reduction concerns and; demonstrate how PrEP can be part of a safe and comprehensive risk management strategy.
Monitoring sheep andCulicoidesmidges in Montana for evidence of Bunyamwera serogroup virus infection
Authors: Johnson Gregory D. , Bahnson Charlie S. , Ishii Patricia , Cochrane Zachary N. , Hokit D. Grant , Plummer Paul J. , Bartholomay Lyric C. , Blitvich Bradley J. 
Published: November 2014

IntroductionA serological and entomological investigation was performed to monitor for potential Bunyamwera (BUN) serogroup virus activity in Montana.ResultsTo facilitate the serological investigation, sera were collected from 104 sheep in 2013 and 2014 and assayed by plaque reduction neutralization test using all six BUN serogroup viruses known to occur in the United States: Cache Valley virus (CVV), Lokern virus (LOKV), Main Drain virus (MDV), Northway virus, Potosi virus and Tensaw virus. BUN serogroup virus‐specific antibodies were detected in 41 (39%) sheep. Of these, three were seropositive for MDV, one was seropositive for CVV, one was seropositive for LOKV and 36 had antibodies to an undetermined BUN serogroup virus. Additionally, 30,606 Culicoides sonorensis were collected in 2013 using Centers for Disease Control and Prevention (CDC) light traps and assayed for cytopathic virus by virus isolation in African Green Monkey kidney (Vero) cells. All midges were negative. Almost one‐third of the midges were further tested by reverse transcription‐polymerase chain reaction using BUN serogroup virus‐reactive primers and all were negative.ConclusionsWe provide evidence of BUN serogroup virus infection in sheep but not C. sonorensis in Montana in 2013‐2014. This study also provides the first evidence of CVV, MDV and LOKV activity in Montana.
Effects of Prostacyclin on Cerebral Blood Flow and Vasospasm After Subarachnoid Hemorrhage
Authors: Rasmussen Rune , Wetterslev Jørn , Stavngaard Trine , Juhler Marianne , Skjøth-Rasmussen Jane , Grände Per Olof , Olsen Niels Vidiendal 
Published: November 2014

Background and Purpose—
Delayed ischemic neurological deficits (DINDs) are a major contributing factor for poor outcome in patients with subarachnoid hemorrhage. In this trial, we investigated the therapeutic potential of prostacyclin, an endogen substance with known effect on vascular tone and blood flow regulation, on factors related to DIND.

Methods—
This trial is a single-center, randomized, blinded, clinical, pilot trial with 3 arms. Ninety patients were randomized to continuous infusion of prostacyclin 1 ng/kg per minute, prostacyclin 2 ng/kg per minute, or placebo. The intervention was initiated day 5 after subarachnoid hemorrhage and discontinued day 10. Primary outcome was the difference in change from baseline in global cerebral blood flow. Secondary outcome measures were occurrence of DIND, angiographic vasospasm, and clinical outcome at 3 months.

Results—
No statistically significant difference in change of global cerebral blood flow was found between the intervention groups. The observed incidence of DIND and angiographic vasospasm was markedly higher in the placebo group, although this difference was not statistically significant. No statistically significant differences in safety parameters or clinical outcome were found between the 3 groups.

Conclusions—
Administration of prostacyclin to patients with subarachnoid hemorrhage may be safe and feasible. Global cerebral blood flow after subarachnoid hemorrhage is not markedly affected by administration of prostacyclin in the tested dose range. It may be possible that the observed reduction in the point estimates of DIND and vasospasm in the prostacyclin groups represents an effect of prostacyclin as this trial was not powered to investigate the effect of prostacyclin on these outcomes.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT01447095.
On the Fullness of Salvation
Authors: Zorgdrager Heleen E. 
Published: November 2014

This essay addresses the need for a fuller, more integral and embodied understanding of salvation in Protestant, especially Reformed theology. Specifically, it makes a case for retrieving the early Eastern Christian notion of theosis/deification for contemporary Reformed theology. After reviewing classical formulations of theosis and other notions of salvation in the broader Christian tradition, it considers conventional Reformed objections to theosis. Then it explores new directions in Calvin research indicated by Carl Mosser, J. Todd Billings, and Julie Canlis, as well as the constructive theology of incarnation presented by Wendy Farley, with a view to determining their potential to assist in this retrieval. In the end, the author formulates some ‘grammar rules’ for articulating theosis in a Reformed-ecumenical, gender-sensitive discourse.
Effect of Diabetes Mellitus on Perioperative Complications and Hospital Outcomes After Ankle Arthrodesis and Total Ankle Arthroplasty
Authors: Schipper Oliver N. , Jiang Jimmy J. , Chen Lan , Koh Jason , Toolan Brian C. 
Published: November 2014

Background: The aim of this investigation was to analyze a nationally representative admissions database to evaluate the effect of diabetes mellitus on the rate of perioperative complications and hospitalization outcomes after ankle arthrodesis (AAD) and total ankle arthroplasty (TAA). Methods: Using the Nationwide Inpatient Sample database, 12 122 patients who underwent AAD and 2973 patients who underwent TAA were identified from 2002 to 2011 based on ICD-9 procedure codes. The perioperative complications and hospitalization outcomes were compared between diabetic and nondiabetic patients for each surgery during the index hospital stay. Results: The overall complication rate in the AAD group was 16.4% in diabetic patients and 7.0% in nondiabetic patients ( P < .001). Multivariate analysis demonstrated that diabetes mellitus was independently associated with an increased risk of myocardial infarction (relative risk [RR] = 3.2, P = .008), urinary tract infection (RR = 4.6, P < .001), blood transfusion (RR = 3.0, P < .001), irrigation and debridement (RR = 1.9, P = .001), and overall complication rate (RR = 2.7, P < .001). Diabetes was also independently associated with a statistically significant increase in length of hospital stay (difference = 0.35 days, P < .001), more frequent nonhome discharge (RR = 1.69, P < .001), and higher hospitalization charges (difference = $1908, P = .04). The overall complication rate in the TAA group was 7.8% in diabetic patients and 4.7% in nondiabetic patients. Multivariate analysis demonstrated that diabetes was independently associated with increased risk of blood transfusion (RR = 9.8, P = .03) and overall complication rate (RR = 4.1, P = .02). Diabetes was also independently associated with a statistically significant increase in length of stay (difference = 0.41 days, P < .001) and more frequent nonhome discharge (RR = 1.88, P < .001), but there was no significant difference in hospitalization charges ( P = .64). Conclusion: After both AAD and TAA, diabetes mellitus was independently associated with a significantly increased risk of perioperative complications, nonhome discharge, and length of hospital stay during the index hospitalization. Level of Evidence: Level III, comparative series.
Approximation methods for solutions of generalized multi-valued mixed quasi-variational inclusion systems
Authors: Lan Heng-you , Li Fang , Abdou Afrah AN , Cho YJ 
Published: November 2014

AbstractThe purpose of this paper is to introduce new approximation methods for solutions of generalized non-accretive multi-valued mixed quasi-variational inclusion systems involving(A,η)-accretive mappings inq-uniformly smooth Banach spaces and, by using the new resolvent operator technique associated with(A,η)-accretive mappings, Nadler’s fixed point theorem and Liu’s inequality, we prove some existence theorems of solutions for our systems by constructing the new Mann iterative algorithm. Further, we study the stability of the iterative sequence generated by the perturbed iterative algorithms. The results presented in this paper improve and generalize the corresponding results of recent works given by some authors.
Predictive models for kidney disease: improving global outcomes (KDIGO) defined acute kidney injury in UK cardiac surgery
Authors: Birnie Kate , Verheyden Veerle , Pagano Domenico , Bhabra Moninder , Tilling Kate , Sterne Jonathan A , Murphy Gavin J 
Published: November 2014

Abstract
Introduction
Acute kidney injury (AKI) risk prediction scores are an objective and transparent means to enable cohort enrichment in clinical trials or to risk stratify patients preoperatively. Existing scores are limited in that they have been designed to predict only severe, or non-consensus AKI definitions and not less severe stages of AKI, which also have prognostic significance. The aim of this study was to develop and validate novel risk scores that could identify all patients at risk of AKI.

Methods
Prospective routinely collected clinical data (n = 30,854) were obtained from 3 UK cardiac surgical centres (Bristol, Birmingham and Wolverhampton). AKI was defined as per the Kidney Disease: Improving Global Outcomes (KDIGO) Guidelines. The model was developed using the Bristol and Birmingham datasets, and externally validated using the Wolverhampton data. Model discrimination was estimated using the area under the ROC curve (AUC). Model calibration was assessed using the Hosmer–Lemeshow test and calibration plots. Diagnostic utility was also compared to existing scores.

Results
The risk prediction score for any stage AKI (AUC = 0.74 (95% confidence intervals (CI) 0.72, 0.76)) demonstrated better discrimination compared to the Euroscore and the Cleveland Clinic Score, and equivalent discrimination to the Mehta and Ng scores. The any stage AKI score demonstrated better calibration than the four comparison scores. A stage 3 AKI risk prediction score also demonstrated good discrimination (AUC = 0.78 (95% CI 0.75, 0.80)) as did the four comparison risk scores, but stage 3 AKI scores were less well calibrated.

Conclusions
This is the first risk score that accurately identifies patients at risk of any stage AKI. This score will be useful in the perioperative management of high risk patients as well as in clinical trial design.
The Harvest suite for rapid core-genome alignment and visualization of thousands of intraspecific microbial genomes
Authors: Treangen Todd J , Ondov Brian D , Koren Sergey , Phillippy Adam M 
Published: November 2014

AbstractWhole-genome sequences are now available for many microbial species and clades, however existing whole-genome alignment methods are limited in their ability to perform sequence comparisons of multiple sequences simultaneously. Here we present the Harvest suite of core-genome alignment and visualization tools for the rapid and simultaneous analysis of thousands of intraspecific microbial strains. Harvest includes Parsnp, a fast core-genome multi-aligner, and Gingr, a dynamic visual platform. Together they provide interactive core-genome alignments, variant calls, recombination detection, and phylogenetic trees. Using simulated and real data we demonstrate that our approach exhibits unrivaled speed while maintaining the accuracy of existing methods. The Harvest suite is open-source and freely available from: http://github.com/marbl/harvest.
Macro- and micronutrient disposition in an ex vivo model of extracorporeal membrane oxygenation
Authors: Estensen Kristine , Shekar Kiran , Robins Elissa , McDonald Charles , Barnett Adrian G , Fraser John F 
Published: November 2014

Abstract

Background
Extracorporeal membrane oxygenation (ECMO) circuits have been shown to sequester circulating blood compounds such as drugs based on their physicochemical properties. This study aimed to describe the disposition of macro- and micronutrients in simulated ECMO circuits.

Methods
Following baseline sampling, known quantities of macro- and micronutrients were injected post oxygenator into ex vivo ECMO circuits primed with the fresh human whole blood and maintained under standard physiologic conditions. Serial blood samples were then obtained at 1, 30 and 60 min and at 6, 12 and 24 h after the addition of nutrients, to measure the concentrations of study compounds using validated assays.

Results
Twenty-one samples were tested for thirty-one nutrient compounds. There were significant reductions (p < 0.05) in circuit concentrations of some amino acids [alanine (10%), arginine (95%), cysteine (14%), glutamine (25%) and isoleucine (7%)], vitamins [A (42%) and E (6%)] and glucose (42%) over 24 h. Significant increases in circuit concentrations (p < 0.05) were observed over time for many amino acids, zinc and vitamin C. There were no significant reductions in total proteins, triglycerides, total cholesterol, selenium, copper, manganese and vitamin D concentrations within the ECMO circuit over a 24-h period. No clear correlation could be established between physicochemical properties and circuit behaviour of tested nutrients.

Conclusions
Significant alterations in macro- and micronutrient concentrations were observed in this single-dose ex vivo circuit study. Most significantly, there is potential for circuit loss of essential amino acid isoleucine and lipid soluble vitamins (A and E) in the ECMO circuit, and the mechanisms for this need further exploration. While the reductions in glucose concentrations and an increase in other macro- and micronutrient concentrations probably reflect cellular metabolism and breakdown, the decrement in arginine and glutamine concentrations may be attributed to their enzymatic conversion to ornithine and glutamate, respectively. While the results are generally reassuring from a macronutrient perspective, prospective studies in clinical subjects are indicated to further evaluate the influence of ECMO circuit on micronutrient concentrations and clinical outcomes.
Purification of DP 6 to 8 chitooligosaccharides by nanofiltration from the prepared chitooligosaccharides syrup
Authors: Dong Huizhong , Wang Yaosong , Zhao Liming , Zhou Jiachun , Xia Quanming , Jiang Lihua , Fan Liqiang 
Published: November 2014

Abstract

Background
Chitooligosaccharides (COS) with degrees of polymerization (DP) 6 to 8 are degraded from chitosan, which possess excellent bioactivities. However, technologies that could purify them from hydrolysis mixtures in the narrow DP range (984 to 1,306 Da) are absent. The objective of this research is to purify DP 6 to 8 COS by nanofiltration on the basis of appropriate adjustments of the feed condition.

Methods
Syrup containing DP 6 to 8 COS at different concentrations (19.0 to 46.7 g/L) was prepared. A commercial membrane (QY-5-NF-1812) negatively charged was applied. Experiments were carried out in full recycle mode, so that the observed COS retentions were investigated at various transmembrane pressures (6.0 to 20.0 bar), temperatures (10°C to 50°C), and pHs (5.0 to 9.0). Then, the feasibility of separation of DP 6 to 8 COS was further studied by concentration ratio under optimum conditions.

Results
The results indicate that the purification of DP 6 to 8 COS by nanofiltration NF is feasible. It was found that the permeate flux was 95.0 L/(m2 h) at 10.0 bar, while it reached to 140.0 L/(m2 h) at 20.0 bar, and it increased with feed temperature, but the membrane pores were also swelled by heating and led to an irreversible wastage of target oligomers. Additionally, the retention behaviors of chitooligosaccharides are significantly influenced by pH.

Conclusions
Although glucosamine and dimer were permeatable at low pH, their retention ratios were remarkably varied from 0.458 to 0.864 when pH was 9.0. With the interaction of hydrogen bonds, structural curling and overlapping of chitooligosaccharides were formed. Consequently, the rejection of chitooligosaccharides at various pHs is variable. Spray-dried products were finally characterized by the matrix-assisted laser desorption/ionization time-of-flight mass spectrum. The spectrum identified the distributions of hexamer, heptamer, and octamer. Combined with high-performance liquid chromatography profiles, the purity and yield of DP 6 to 8 chitooligosaccharides were up to 82.2% and 73.9%, respectively.
Regulation of CXCR2 expression and function by a disintegrin and metalloprotease-17 (ADAM17)
Authors: Mishra Hemant K , Long Chunmei , Bahaie Nooshin S , Walcheck Bruce 
Published: November 2014

Abstract
The chemokine receptor CXCR2 is expressed at high levels on circulating neutrophils and is critical for directing their migration to sites of inflammation. CXCR2 surface levels are rapidly modulated by 2 mechanisms—cell internalization and recycling upon ligand binding—and by a metalloprotease activity following overt neutrophil activation by nonligand stimuli. The latter process has only been described in human neutrophils, and essentially, nothing is known about its functional relevance and the specific protease involved. We show that targeting ADAM17 in mouse and human neutrophils blocks CXCR2 down-regulation induced by nonligand stimuli but not by chemokine ligands. This was determined by use of a selective ADAM17 inhibitor, an ADAM17 function-blocking antibody, and ADAM17 gene-targeted mice. CXCR2 is known to undergo a marked down-regulation during various inflammatory disorders, and this is associated with impaired neutrophil recruitment. We show that blocking ADAM17 activity reduced CXCR2 down-regulation on circulating neutrophils and enhanced their recruitment during acute inflammation, which was reversed by a CXCR2 inhibitor. Taken together, our findings demonstrate that unlike CXCR2 internalization, ADAM17 induction down-regulates the receptor in an irreversible manner and may serve as a master switch in controlling CXCR2 function, but may also contribute to neutrophil dysfunction during excessive inflammation.
Selecting relevant nodes and structures in biological networks. BiNAT: a new plugin for Cytoscape
Authors: Cumbo Fabio , Felici Giovanni , Bertolazzi Paola 
Published: November 2014

Summary: In order to understand a network function, it’s necessary the understanding of its topology, since the topology is designed to better undertake the function, and the efficiency of network function is influenced by its topology. For this reason, topological analysis of complex networks has been an intensely researched area in the last decade.Results: Here we propose BiNAT, a Cytoscape [1] plugin able to perform network analysis, providing a full set of useful tools to discover the most significant nodes and structures in a network.Conclusions: The plugin has been approved on the official Cytoscape plugins repository and it is downloadable directly from this site: http://dmb.iasi.cnr.it/binat.php where a full guide is also available.
Marketplace or Reseller?
Authors: Hagiu Andrei , Wright Julian 
Published: November 2014

Intermediaries can choose between functioning as a marketplace (in which suppliers sell their products directly to buyers) or as a reseller (by purchasing products from suppliers and selling them to buyers). We model this as a decision between whether control rights over a noncontractible decision variable (the choice of some marketing activity) are better held by suppliers (the marketplace mode) or by the intermediary (the reseller mode). Whether the marketplace or the reseller mode is preferred depends on whether independent suppliers or the intermediary have more important information relevant to the optimal tailoring of marketing activities for each specific product. We show that this trade-off is shifted toward the reseller mode when marketing activities create spillovers across products and when network effects lead to unfavorable expectations about supplier participation. If the reseller has a variable cost advantage (respectively, disadvantage) relative to the marketplace, then the trade-off is shifted toward the marketplace for long-tail (respectively, short-tail) products. We thus provide a theory of which products an intermediary should offer in each mode. We also provide some empirical evidence that supports our main results. Data, as supplemental material, are available at http://dx.doi.org/10.1287/mnsc.2014.2042 . This paper was accepted by Bruno Cassiman, business strategy.
‘The apotheosis of man, the forgotten peacock’
Authors: Takis  
Published: November 2014

Abstract
The interdisciplinary practice-based research project, ‘The apotheosis of man, the forgotten peacock; from deconstruction to reconstruction to re-proposal of the male suit’, through a series of workshops and an interactive performance installation, aims to challenge the persisting conventional tradition of designing and wearing the male suit, proposing alternative perspectives by researcher – performance designer. The investigation focuses on the tools and means of processing the male suit in practice. It explores how to overcome conventions, generate artistic ideas, and how to apply this to something wearable. This visual essay demonstrates how the outcomes of three workshops fed on the creation of the experimental research driven suits. In all the workshops the participants were set the task to create a series of male garments by questioning and reinterpreting the notion of the masculinity and by using concepts and methods representative of deconstruction. Every participant designed and made a male garment by recycling a male suit jacket. The first presented workshop took place in May 2007, at the University of the Arts Bucharest, Romania and the participants were the second-year fashion design students. The second took place in September 2013 at the ‘World Stage Design Exhibition’, as part of the ‘Costume in Action series – Upcycling Costume: DeReconstructing Masculinity’. The participants were a mixture of student and professional international performance designers. The final two pages demonstrate the Plus Series suits, based on the addition of design elements to the suit.