bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Topically Applied Spironolactone on Androgen Stimulated Sebaceous Glands in the Hamster Pinna
Authors: Seki Taisuke , Toyomoto Takashi , Morohashi Masaaki 
Published: April 2015

AbstractThe effects of spironolactone (5% SYC‐201G, a preparation developed for clinical use in acne vulgaris by Searle Yakuhin K.K.), which is known to have antiandrogenic effects by competitively inhibiting dihydrotestosterone at androgen receptor sites, was topically applied to the androgen stimulated sebaceous glands of adult female golden hamsters.Androgen stimulation, induced by intramuscular injection of testosterone propionate (TP) every other day over a two week period, resulted in a 2.5 to 2.7 time increase in the size of the sebaceous glands of the hamster pinna.Once‐daily treatment with 5% SYC‐201G or matching placebo was applied to androgen‐stimulated hamsters on one pinna only during the same period as TP injection. Comparison between the treated and untreated sides revealed a significant suppression in the sebaceous gland size (p<0.05) by 5% SYC‐201G; no such effect was observed with placebo. The difference in the suppression rate of the sebaceous gland size between 5% SYC‐201G (23%) and matching placebo (–4.7%) was significant (p<0.01).
Cutaneous Eruption in a Patient with Cardiac Myxoma
Authors: Abraham Zeev , Rozenbaum Michael , Rosner Itzhak , Odeh Majed , Oliven Arie 
Published: April 2015

AbstractAtrial myxoma was diagnosed in a young woman who had cutaneous and constitutional manifestations clinically suggestive of cutaneous vasculitis without cardiac signs or symptoms. Scrupulous physical and laboratory examinations excluded additional medical disorders. The patient was totally cured by surgical removal of the tumor. Various aspects of the association of cardiac myxoma with dermatological findings are discussed, emphasizing the importance of echocardiography in the diagnostic workup of various skin abnormalities, including unexplained vasculitis, even in the absence of cardiac findings.
Disseminated Eruptive Nevocellular Nevi
Authors: Bong Ha Wook , Lee Seok June , Lee Kwang Hoon , Chung Kee‐Yang 
Published: April 2015

AbstractA 13‐year‐old boy suddenly developed about 2,000 dark brown to black colored papules on his face and neck and about 500 lesions on his trunk and upper extremities during a six month period. Histopathologic features were compatible with junctional nevus. The results of alpha melanocyte stimulating hormone (MSH), proliferating cell nuclear antigen (PCNA), and epidermal growth factor receptor (EGF/R) studies are presented. To the best of our knowledge, this report represents an outbreak of the highest number of nevocellular nevi in a short period without any malignant nature or evident triggering factor.
Production and Secretion of Platelet‐Derived Growth Factor AB by Cultured Human Keratinocytes: Regulatory Effects of Phorbol 12‐Myristate 13‐Acetate, Etretinate, 1,25‐Dihydroxyvitamin D3, and Several Cytokines
Authors: Zhang Jian‐Zhong , Maruyama Kohji , Ono Ichiro , Kaneko Fumio 
Published: April 2015

AbstractPlatelet‐derived growth factor (PDGF) is a potent mitogen for several mesenchymal cells and plays an important role in wound repair. Three PDGF isoforms, PDGF‐AA, PDGF‐BB, and PDGF‐AB, have been found to be generated in various tissues. PDGF‐AB production by normal human keratinocytes (NHKs), by human squamous cell carcinoma cell line (HSC‐1) cells, and by human dermal fibroblasts (HDFs) was studied in the presence of agents which influence cell growth. Both NHKs and HSC‐1 cells spontaneously produced and secreted PDGF‐AB. NHKs grown in keratinocyte growth medium produced more PDGF‐AB than did those grown in keratinocyte basic medium. Phorbol 12‐myristate 13‐acetate inhibited PDGF‐AB production in NHKs but promoted its production in HSC‐1 cells. 1,25‐dihydroxyvitamin D3 up‐regulated PDGF‐AB production, whereas etretinate did not. High levels of calcium in the culture medium induced little change in cellular PDGF‐AB levels. Prostaglandin E1 slightly inhibited PDGF‐AB production, transforming growth factor β1 promoted PDGF‐AB production and interferon‐γ, interleukin‐1α, and tumor necrosis factor‐α failed to exert any influence at all. Cultured HDFs did not produce any detectable PDGF‐AB. These results suggest that keratinocytes are a major source of cutaneous PDGF and that this factor may therefore play an important role in wound repair and in certain proliferative skin diseases.
Clinical and Histopathologic Analysis of the Relationship between Lichen Planus and Chronic Hepatitis C
Authors: Tanei Ryoji , Watanabe Kiyoharu , Nishiyama Shigeo 
Published: April 2015

AbstractA prospective clinical investigation of 45 patients with lichen planus (LP) demonstrated a significant association between LP and chronic hepatitis C. Anti‐hepatitis C virus (HCV) antibodies were found in 17 (37.8%) of the 45 LP patients. This was significantly higher than in the controls. This higher prevalence of anti‐HCV antibodies was found equally in both male and female patients in the three types of LP; cutaneous only type, mucous only type, and both cutaneous and mucous type. Most of the patients with positive anti‐HCV antibodies had abnormal values of transaminase enzymes and/or a past history of chronic hepatitis. Histological and immunohistological investigations of three cases with LP and chronic hepatitis C demonstrated some morphologic similarities between these two diseases. Histopathologic findings of both LP and chronic hepatitis C were based on a T lymphocytic infiltrate with keratinocyte or hepatocyte damage. The degrees of infiltrating cells positive to UCHL‐1, MX‐panB, Leu‐7, and human leukocyte antigen (HLA)‐DR antibodies in the chronic hepatitis C lesions seemed to be similar to those in the LP lesions. These results may support a possible relationship between LP and chronic hepatitis C and the hypothesis that LP may be associated with chronic liver diseases as a result of a cytotoxic attack on the hepatocytes.
Skin Manifestations Associated with Rheumatoid Arthritis
Authors: Yamamoto Toshiyuki , Ohkubo Hiroko , Nishioka Kiyoshi 
Published: April 2015

AbstractWe examined cutaneous manifestations of rheumatoid arthritis (RA) of 142 Japanese patients who visited both the Departments of Dermatology and Rheumatology of our hospital. We classified cutaneous lesions into specific and/or characteristic or nonspecific ones. Nonspecific lesions predominated in our series. Among the specific skin manifestations, which comprised 10% of the total, rheumatoid nodules, rheumatoid papules, rheumatoid neutrophilic dermatitis, and severe vasculitic ulcers correlated with high titers of rheumatoid factors and progression of RA, while purpura and livedo did not. Nonspecific skin manifestations failed to correspond with the level of rheumatoid factors. Among the nonspecific lesions, asteatotic eczema, candida interdigitalis, and tinea unguium were commonly detected.
Extensive Aberrant Mongolian Spot
Authors: Park Kyung Deuk , Choi Gwang Seong , Lee Kwang Hoon 
Published: April 2015

AbstractA 9‐month‐old male infant had generalized diffuse blue‐gray pigmentation over most of his body, sparing the scalp, face, neck, palms, soles, periumbilical area, genital area, and nipples. Within the lesion, there were several conspicuous macules of considerably darker hue. Histologic examination revealed numerous dermal melanocytes. By 16 months of age, the child's blue‐gray pigmentation had decreased substantially.
Destructive Encephalopathy in Incontinentia Pigmenti: A Case Report
Authors: Yang Jen‐Hang , Ma Shao‐Yin , Tsai Chang‐Hai 
Published: April 2015

AbstractWe report a 2‐day‐old neonate with incontinentia pigmenti combined with destructive encephalopathy. Generalized seizures developed soon after birth; characteristic papulovesicles and hyperpigmented linear streaks appeared several days later. The brain CT scan showed multiple low density areas with cortical atrophy and ventricular enlargement. Histopathological examination favored the diagnosis of incontinentia pigmenti. Although a series of microbiological examinations failed to identify any infectious agent, the patient was still treated vigorously with anticonvulsants, systemic antibiotics, and acyclovir from the beginning of hospitalization. However, the seizures persisted, and the patient died 26 days after birth. Our report suggests that incontinentia pigmenti with encephalopathy may have a fatal prognosis.
Nasal Myiasis in Leprosy Leading to Unusual Tissue Destruction
Authors: Thami Gurvinder Pal , Baruah Manik Chandra , Sharmce Subhash Chondee , Behera Niranjan Kumar 
Published: April 2015

AbstractNasal mucosal involvement is a constant feature in lepromatous leprosy (1). Granulomatous infiltration of nasal mucosa and consequent sensory loss may result in atrophic rhinitis and or painless ulceration, which is usually asymptomatic except for later complications of epistaxis, septal perforation, inflammation, and/or myiasis in long neglected patients. An unusual destruction of nasal architecture mimicking cancrum oris caused by myiasis in lepromatous leprosy is described for its rarity.
Topical 5‐Fluorouracil Ointment for Senile Comedones: A Case Report
Authors: Yoshinaga Keiichiro , Kanzaki Tamotsu 
Published: April 2015

AbstractThe usefulness of topical vitamin A acid for senile comedones was reported by Kligman et al. in 1971 (1). It is, however, not available in Japan. We treated senile comedones with topical 5‐fluorouracil ointment and found that it was very effective.
Immunoglobulin Gene Analysis of Cutaneous Pseudolymphoma by Polymerase Chain Reaction
Authors: Nakayama Fumiaki , Kurosu Katsushi , Yumoto Norio , Mikata Atsuo 
Published: April 2015

AbstractPolymerase chain reaction (PCR) was used to amplify the V‐D‐J region of the immunoglobulin heavy chain gene from DNA extracted from the formalin fixed, paraffin embedded skin of 3 cases of pseudolymphoma. The products were electrophoresed and observed under ultraviolet light after ethidium bromide staining. Specimens of two cases showed smears of polyclonal amplified DNA. The specimen of one case (Case 3), however, showed a single band with a smear. The presence of monoclonality in B lymphocyte populations may suggest the possibility of low grade malignancy of pseudolymphoma or transformation to malignant lymphoma in the future.
Immunoglobulins Specific to Mosquito Salivary Gland Proteins in the Sera of Persons with Common or Hypersensitive Reactions to Mosquito Bites
Authors: Shan En‐zhi , Taniguchi Yoshiki , Shimizu Masayuki , Ando Katsuhiko , Chinzei Yasuo , Suto Chiharu , Ohtaki Tetsuya , Ohtaki Noriko 
Published: April 2015

AbstractUsing the immunoblot technique, we analyzed the quality and quantity of IgG, IgG4, and IgE specific to mosquito salivary gland (hereafter abbreviate as SG) components of Aedes albopictus in the sera of volunteers with common reactions and of 3 patients with severe reactions.In the volunteers with delayed reactions only or with both delayed and immediate reactions, IgG against SG components of A. albopictus formed several faint or moderately stained bands. Those with immediate reactions showed several intense bands and many other weak bands. In volunteers, who had been bitten by Aedes sp. frequently but had no skin reaction, and in severe cases, many intense IgG bands were observed. IgG4 bound to SG components were found in the sera of the common reaction group at the levels of 24 and 48 kD, but, in one severe case, no bands were observed, although the total IgG was very high. IgE levels specific to SG components were much higher in severe cases than in the volunteers.These results indicate that high titers of specific IgG and IgE and lack of IgG4 for particular components of SG may lead to severe allergic reactions in severe cases. Immunoblotting analysis of the antibodies also verified the possibility of developing in vitro tests to identify causative species of the mosquito for severe cases.
Generalized Lichen Nitidus Appearing Subsequent to Lichen Planus
Authors: Kawakami Tamihiro , Soma Yoshinao 
Published: April 2015

AbstractA 27‐year‐old man was seen with multiple, small, shiny papules on his shoulders, upper arms, and trunk, and hyperpigmented violaceous plaques on his feet. The former was diagnosed as generalized lichen nitidus and the latter, as lichen planus. It is not likely that the coexistence of the two diseases in this patient is a fortuitous one, since generalized lichen nitidus is a very rare condition. The association of lichen nitidus and lichen planus suggests that lichen nitidus is closely related to lichen planus and that the two diseases may be different manifestations of essentially the same pathogenetic process.
Correlation of Lesional Skin Corneometry Values with Serum E‐Selectin Levels and Disease Severity in Patients Affected by Plaque‐type Psoriasis: Recovery after Effective Therapy
Authors: Carducci Massimo , Mussi Anna , Bonifati Claudio , Tomaselli Roberta , Onorati Maria Teresa , Trento Elisabetta , Ameglio Franco 
Published: April 2015

AbstractTen subjects with plaque‐type psoriasis (5 females, 5 males, median age 47, range 20–71 years, median psoriasis area and severity index [PASI] score=15.5, range 8.1–23.0) were observed before (week 0) and at the second and sixth week after starting treatment (PASI=10.5, range 7.8–14.6; PASI=8.5, range 1.6–11.6, respectively).At each of these times, the patients were evaluated for both lesional and unlesional skin corneometry and serum E‐selectin values, previously shown to be increased in psoriatic subjects.At time 0, both the corneometry and the E‐selectin values were significantly correlated with the PASI, infiltration and desquamation scores. As expected, the corneometry was statistically higher in the unlesional than in the lesional areas (p<0.001).After therapy, the lesion improvement was related both to the PASI scores and E‐selectin level decreases (from median levels of 15.5 to 8.5 and from 18 to 13.2 ng/ml, respectively, p<0.05) as well as to the corneometric level increases (from a median value of 34.5 to 42, p<0.05). Considering the data obtained at all of these times, significant correlations were found between the PASI scores, lesional skin corneometry and serum E‐selectin levels.In conclusion, lesional corneometry seems to represent an objective alternative method for reliably monitoring psoriatic patients.
Human Type VI Collagen: Purification from Human Subcutaneous Fat Tissue and an Immunohistochemical Study of Morphea and Systemic Sclerosis
Authors: Takasaki Shuji , Fujiwara Sakuhei , Shinkai Hiroshi , Ooshima Akira 
Published: April 2015

AbstractCollagen was isolated from human placenta by pepsin digestion and salt precipitation. This collagen was similar in its electrophoretic mobility and immunological reactivity with monoclonal antibody to form B of type VI collagen in the literature (Trueb B, Schreier T, Bruckner P and Winterhalter K. 1987. Eur. J. Biochem. 166: 699–703). We prepared polyclonal rabbit antiserum against α2 chain of type VI collagen and performed an immunohistochemical study using this polyclonal antibody. It reacted in fat tissue and around vessels and peripheral nerves in normal human skin. To confirm the presence of type VI collagen in fat tissue, we isolated collagen from human subcutaneous tissue. This collagen showed a similar pattern in polyacrylamide gel electrophoresis with that from human placenta and cross‐reacted with monoclonal or polyclonal antibody against type VI collagen. By immunohistochemical staining, abundant type VI collagen was observed in the septum of subcutaneous fat tissue in morphea or systemic sclerosis. In the mild hyalinizing areas or after treatment with 6M urea or hyaluronidase in highly hyalinized areas, the staining of type VI collagen increased. These data suggest that the amount of type VI collagen in subcutaneous tissue is involved in the early phases of these fibrosing disorders and that type VI collagen accumulates even more in hyalinizing tissue in late phases of these diseases.
“Malignant Spitz Nevus” in a 2‐Year‐Old Japanese Child
Authors: Nitta Yukiko , Ohkochi Yasuyuki , Hara Kazuo 
Published: April 2015

AbstractSmith et al. (1989) have reported a variant of Spitz's nevus with histological atypical features. Despite local lymph node metastases, further metastases were not observed. They proposed the name “malignant Spitz nevus” for this variant.A 2‐year‐old Japanese girl had a large nodule (27 × 17 mm) surrounded by an indurated erythema over the Achilles tendon. Histologically, it proved to be a melanocytic lesion resembling spindle cell and epithelioid cell nevus (Spitz's nevus) with unusual features; the tumor extended deep into the subcutis, and the mitotic figures deep into the tumor, together with prominent lymphatic vessel invasion by melanocytes. Thus the tumor was aptly termed “malignant Spitz nevus”. Flow cytometric analysis of the DNA content revealed a diploid pattern. The child is well 5 years after a wide resection of the tumor. The diploid pattern of the DNA content as well as the good prognosis could support the idea that “malignant Spitz nevus” fits within the spectrum of Spitz's nevus.
Successful Treatment of Severe Arthralgia Associated with Palmoplantar Pustulosis with Low‐Dose Oral Cyclosporine A
Authors: Yamamoto Toshiyuki , Kimura Kyouko , Katayama Ichiro , Nishioka Kiyoshi 
Published: April 2015

AbstractTwo patients with severe arthralgia associated with palmoplantar pustulosis (PPP) were treated with oral cyclosporine A (CsA). Clinical efficacy was assessed on a 0–4 point scale for erythema, desquamation, infiltration, and pustulation, and on a 0–3 point pain scale. Skin lesions and arthralgia improved within twelve weeks with low dose CsA ranging from 2.1 to 2.2 mg/kg/day. High levels of plasma interleukin‐6 (IL‐6) were reduced to the normal range.
Androgen Status in Adolescent Women with Acne Vulgaris
Authors: Aizawa Hiroshi , Nakada Yoshiko , Niimura Michihito 
Published: April 2015

AbstractAndrogens are essential for the development of acne. The object of this study was to elucidate the androgen status of women with adolescent (Tanner's stage IV–V) acne alone and compare them to age‐matched normal controls. We measured serum levels of total testosterone (T), free testosterone (FT), dihydrotestosterone (DHT), and dehydroepiandrosterone sulfate (DHEA‐S) in 15 women with adolescent acne and 13 age‐matched healthy controls. No significant differences were found between the mean levels of T, FT or DHT levels in patients and controls. However, the mean levels of DHEA‐S in the patient population (1886 ± 829 ng/ml) were significantly (p<0.05) higher than normal controls (1287 ± 620 ng/ml). There was also no correlation between androgen levels and acne severity. Thus it is unlikely that serum androgens play a principal role in women with adolescent acne.
Differential Cell‐ and Immuno‐Biological Properties of Murine B16‐F1 and F10 Melanomas: Oncogene c‐fos Expression, Sensitivity to LAK Cells and/or IL‐2, and Components of Gangliosides
Authors: Nakayama Juichiro , Urabe Kazunori , Tsuchida Tetsuya , Urabe Atsumichi , Terao Hiroshi , Taniguchi Shun'ichiro , Hori Yoshiaki 
Published: April 2015

AbstractDifferential cell‐ and immuno‐biological properties of two murine melanoma B16 variants, B16‐F1 and F10, were investigated. Studies focused on the expression of proto‐oncogene c‐fos, sensitivities to LAK cells and/or IL‐2, and modulation of the expression of ganglioside components after treatment with IL‐2. Proto‐oncogene c‐fos was found to be highly expressed in F10 lines by an in situ hybridization technique and also in F10 lung metastatic nests by immunofluorescent staining with anti‐c‐fos antibody. F1 melanomas were more sensitive to local injection of IL‐2. F10 melanomas hardly responded to IL‐2 treatment, but successive injections of a combination of LAK cells and IL‐2 did cause prolongation of survival rates, even of F10 melanoma‐burdened mice. A major component of gangliosides of both F1 and F10 melanomas was GM3. Production of GM3 in F10 melanomas treated with IL‐2 for 4 days increased, and, if the treatment was continued for 7 days, minor components of gangliosides, such as GM2, GM1, and GD1a, appeared only in F1 melanomas, while the increase of production of GM3 disappeared in both melanomas. These experimental results may provide clues for additional mechanisms which allow these two murine melanoma variants to show different implantation and metastasis rates.
Serial Cultivation of Human Nail Matrix Cells under Serum‐free Conditions
Authors: Nagae Hiroaki , Nakanishi Hideki , Urano Yoshio , Arase Seiji 
Published: April 2015

AbstractWe have established serial cultures of human nail matrix cells (NMCs) under serum‐free conditions. We cultured NMCs using two different methods depending upon the volume of nail matrix obtained. When a sufficient amount of nail matrix was obtained, they were minced and treated with 0.25% trypsin and 0.03% EDTA. The NMCs were transferred directly as a dispersed cell culture into KGM medium. Because a sufficient amount of matrix was rarely obtained, we developed a method by which NMCs were cultured primarily as implanted small matrices in Eagle's MEM (high Ca# medium) supplemented with 15% fetal bovine serum for the first 4 to 5 days; during this time, the NMCs expanded from the matrices and formed colonies around them. NMCs then were cultured with KGM. In both methods, KGM medium supported the growth of NMCs without a biological feeder layer. These cells could be cultivated serially for at least seven passages. Half of the cells were positively stained with a monoclonal antibody against hair (hard) keratin which is expressed in nail matrix in vivo, indicating that the cells originated from the nail matrix. These methods will now permit investigations of nail matrix cells that previously were unfeasible because of the relative lack of cells and difficulties with propagation.
Successful Treatment of Dark‐colored Epidermal Nevus with Ruby Laser
Authors: Baba Takako , Narumi Hiromi , Hanada Katsumi , Hashimoto Isao 
Published: April 2015

AbstractThe pulsed ruby laser has a selective thermolytic effect. Recently, it has been available for the treatment of superficial pigmented disorders. We studied 5 cases of epidermal nevus treated with the pulsed ruby laser. In comparison with the usual methods including electrocautery, cryotherapy and skin abrasion, ruby laser therapy is an excellent tool due to technological ease and rapid improvement. Depigmentation after treatment in 2 cases was the only side effect of this therapy. Bose cases had a dark pigmentation of the skin. Despite of the risk of discoloration, the ruby laser is one of the most effective tools for therapy of pigmented epidermal nevus.
Immunofluorescence Study of Pemphigus from North India
Authors: Kumar Surendra , Thappa Devinder Mohan , Sehgal Shobha 
Published: April 2015

AbstractBoth serum studies by indirect immunofluorescence (IIF) tests and skin biopsy examination by direct immunofluorescence (DIF) were performed on 22 cases of pemphigus with clinically active lesions. Twenty cases had pemphigus vulgaris and two, pemphigus foliaceus. The majority of cases (70%) were between 21 and 60 years old; the mean age was 39.5 ± 12.7 years. There was no sex predilection. DIF showed the positive fluorescence of intercellular cement substance (ICS) of the epidermis in all 22 cases (100%). IgG was positive in 77.27%, followed sequentially by C3C (50%), IgA (45.45%), and IgM (36.36%). Six cases (27.27%) also showed granular immunoglobulin and/or complement deposits at the dermoepidermal junction. IIF detected serum antiepithelial antibodies in 18 cases (81.81%) using human esophagus as substrate. Most of these cases (88.88%) showed IgG type of antibodies; the other 11.11% exhibited IgA and IgM in low concentrations. 1+ titer positivity was observed in 15 cases. This study demonstrates the value of DIF for a definitive diagnosis of pemphigus. However, it is also important to appreciate that immunofluorescence is not a substitute for histopathology, but rather complementary to it.