bluuebunny/crossref_metadata_2025_split huggingface dataset:
CHEMOIMMUNOTHERAPY FOR DISSEMINATED MALIGNANT MELANOMA WITH DTIC, ACNU, VCR AND OK432
Authors: Chiba Masahito , Jimbow Kowichi , Kizukuri Kei , Homma Kouichi 
Published: April 2015

ABSTRACTThree out of fifteen cases of disseminated malignant melanoma with complete or partial responses to our combined chemoimmunotherapy, DTIC, ACNU, VCR and OK432, are reported. Case 1 responded completely with disappearance of skin and brain lesions. Case 2 responded partially to a lung metastasis. Case 3 responded totally with the complete disappearance of lung and brain metastatic lesions. None of these cases suffered any significant side effects during the trial.
INTRAEPIDERMAL FREE NERVE ENDINGS RELATING TO EPIDERMAL MELANOCYTES IN SPOTTED GUINEA PIGS
Authors: Mihara Motoyuki , Hashimoto Ken , Kumakiri Masanobu 
Published: April 2015

ABSTRACTIntraepidermal free nerve endings were studied in spotted (black and white) guinea pigs. Specimens were obtained from black, gray, and white areas. Twenty blocks were made from each area and sections were examined by electron microscopy. Intraepidermal free nerve endings were found in the interfollicular epidermis of all three areas. The distribution density for the black areas was about twice that for the white areas. The frequency in the gray areas was intermediate between that for the black areas and that for the white areas. Intraepidermal free nerve endings contacted the melanocytes directly in the interspaces between the basal lamina of the epidermis and the cytomembrane of the melanocyte or the dendrite of the melanocyte in the intercellular spaces between basal keratinocytes. In most cases, their axons were ensheathed by Schwann cells. In some cases, a part of the axon expanded like a balloon about 200–300 nm in diameter. The ballooning structure contained several vacuoles about 40–60 nm in diameter. These vacuoles seemed to have been secreted into the extracellular spaces. In such cases, an accumulation of dense materials was observed along the opposed membrane of the vacuole and the cytomembrane of the melanocyte. In other cases, the cytomembranes of axons and those of melanocytes seemed to form synapse‐like structures.
EXPERIMENTAL STUDY OF DIFFERENCES IN HUMAN SOLE SKIN BETWEEN ONE CYCLE METHOD AND TWO CYCLE METHOD AFTER SPRAY CRYOSURGERY
Authors: Ueda Keiichi , Komori Yasushi , Wakabayashi Shunji 
Published: April 2015

ABSTRACTUltrastructural investigations were performed on human sole skin after spray cryosurgery with liquid nitrogen, using a one cycle (single freeze and thaw) method and a two cycle (double freeze and thaw) method.In the one cycle method, the epidermal cells from the basal cell layer to the spinous cell layer showed characteristic changes, i.e., nuclear chromatin forming condensed masses, the appearance of remarkable perinuclear edematous regions, tonofibrils pushed out towards the periphery of the cells and narrow intercellular spaces with intact desmosomes.In the two cycle method, homogeneous, less dense areas were seen in the nuclei of epidermal cells from the basal cell layer to the spinous cell layer. Wide, less dense, edematous, perinuclear areas were seen in which organelles, i.e. mitochondria, endoplasmic reticulum, and vacuoles were located. Tonofibrils were pushed out to the periphery of the cells. Complete cryonecrosis was observed in cells from the basal layer to the spinous layer.Ultrastructural changes differed in degree between the one cycle freezing method and the two cycle freezing method after spray cryosurgery.It was concluded that the cryosurgical technique using the two cycle method was more effective in inducing complete cryonecrosis.
TWO FORMS OF ADENOSINE DEAMINASE IN PIG EPIDERMIS (II)
Authors: Koizumi Hiroko , Iizuka Hajime , Aoyagi Takashi , Miura Yusho 
Published: April 2015

ABSTRACTUsing pure pig skin epidermis, we investigated the biochemical nature of adenosine deaminase, Two different (large and small) forms of adenosine deaminase were separated by a gel filtration technique using Sephadex G‐150 column chromatography. The molecular weight of the large form was 300,000 to 350,000 and that of the small form was 30,000 to 40,000.The kinetics of the two enzymes were quite similar, in terms of Km values for adenosine, substrate specificity, pH optima, temperature sensitivity, isoelectric points, and inhibition by coformycin, a tight‐binding inhibitor of adenosine deaminase.
LIPODYSTROPHY CENTRIFUGALIS ABDOMINALIS INFANTILIS IN DIZYGOTIC TWINS
Authors: Mizoguchi Masako , Nanko Shinichiro 
Published: April 2015

ABSTRACTWe examined a set of three‐year‐old Japanese dizygotic twins with lipodystrophia centrifugalis abdominalis infantilis. One had depressed skin lesions on the left side of her neck and in her axillary area, while the other had a lesion on her right inguinal area.Although their MLC were identical, they differed from the MLC of an unrelated patient who suffered from the same disease. HLA‐BW 51 was found in all three patients.
AN UNUSUAL CASE OF CUTANEOUS PLASMACYTOSIS
Authors: Aso Miki , Shimao Shuhei 
Published: April 2015

ABSTRACTA seventy‐five year old man has had numerous papules over his entire body for about 50 years without any systemic involvement. Histological examination revealed that many plasma cells and a few lymphoid cells had infiltrated into the dermis. The plasma cells did not show any atypia. Laboratory examinations, including Bence‐Jones' protein, serum gammaglobulin, and immunoglobulins, demonstrated no abnormal findings. We consider this case not to be a true tumor but rather a benign reactive proliferation of plasma cells in the skin, although its etiology is unclear.
WORINGER‐KOLOPP DISEASE
Authors: Imai Seiji , Nitto Heita , Oka Kichiro 
Published: April 2015

AbstractA case of Woringer‐Kolopp disease was studied using electron microscopy. The patient was a 45‐year‐old Japanese woman with a plaque lesion of six years duration on the left upper thigh. The pathological features of the lesion included abnormal cells, mainly in the epidermis and compatible with those of Woringer‐Kolopp disease.Electron microscopic examination indicated that the abnormal cells within the epidermis and passing through the basal lamina had features indicating a histiocytic origin, including abundant and protruded cytoplasm and lysosomal dense bodies.
YELLOW MACULES APPEARING DURING CORTICOSTEROID TREATMENT OF SYSTEMIC LUPUS ERYTHEMATOSUS
Authors: Aso Miki , Mihara Motoyuki , Shimao Shuhei 
Published: April 2015

AbstractA 42‐year‐old woman who had been treated with corticosteroid for systemic lupus erythematosus had many yellow macules on her extremities and body. Light microscopic examination revealed a decrease or disappearance of collagen fibers and the formation of abnormal fibers. Ultrastructural study showed that these abnormal fibers were numerous cord‐like substances related to extracellular amorphous or filamentous materials and similar to the amorphous materials in the rough endoplasmic reticulum of fibroblasts.It was suggested that fibroblasts injured by corticosteroid produced an abnormal precursor of collagen fiber and that the synthesis of normal collagen fibers was inhibited in the yellow macules.
FRACTIONATION AND CHARACTERIZATION OF THE HUMAN EPIDERMAL STRATUM CORNEUM IN KERATINIZATION DISORDERS
Authors: Yoshiike Takashi , Negi Makoto , Manabe Motomu , Hattori Michihiro , Ogawa Hideoki 
Published: April 2015

AbstractStratum corneum obtained from persons with a variety of keratinization disorders was fractionated into keratin fibers, water soluble proteins and cell membranes. SDS gel electrophoresis was used to study the keratin fibrous and water soluble fractions, and amino‐acid analysis was employed for the membraneous fractions.Electrophoresis of the keratin fibrous fractions obtained from cases of epidermolytic hyperkeratosis and psoriasis vulgaris revealed a significant decrease in the 55,000 dalton subunit was observed. Similarly, a reduction of the 73,000 dalton subunit was observed in samples from a case of keratoma climacterium. Minor variations from the normal were recorded from cases of ichthyosis vulgaris, lamellar ichthyosis, and palmoplantar keratosis (which included a Vörner and punctate type, and one with corneal dystrophy).The water soluble fractions from all the cases of keratinization disorders studied exhibited different densities for the individual polypeptides after electrophoresis.The most impressive changes were revealed by amino‐acid analysis of the membraneous fractions. Half‐cystine and proline were uniformly reduced in cases of ichthyosis vulgaris, lamellar ichthyosis, punctate palmo‐plantar keratosis, palmoplantar keratosis with corneal dystrophy and keratoma climacterium, and the presence of an abnormal amino‐acid, ornithine, was characteristic of epidermolytic hyperkeratosis and psoriasis vulgaris.
SKIN TUMORS OF XERODERMA PIGMENTOSUM (I)
Authors: Kobayashi Misaki , Satoh Yoshiaki , Irimajiri Toshikatsu , Mitoh Yoshihiro , Kozuka Takehito , Sato Kenji , Ichihashi Masamitsu , Nakanishi Takafumi 
Published: April 2015

AbstractSixty‐eight xeroderma pigmentosum (XP) patients, including 41 of Group A and 27 of XP‐variant, were examined for skin malignancies such as squamous cell carcinoma (SCC), basal cell epithelioma (BCE), and malignant melanoma (MM).Approximately half of the patients developed skin malignancies on sunlight‐exposed areas. The most frequent skin malignancy in both groups was BCE. In this survey, two cases with MM were found for the first time in Japan, one in Group A and one in XP‐variant. The most important difference between Group A and XP‐variant was in the age of onset of skin malignancies. The average age at the first excision of skin malignancies was 9 in Group A, while that of XP‐variant was 32. In Group A, the youngest case was 3 years old, and most of the cases with skin malignancies were younger than 15. In XP‐variant, on the contrary, the youngest case was 15 and most cases were older than 20. XP‐variant patients were apt to have multiple lesions of BCE. The ratio of BCE to SCC was about 3:1 in Group A, and 8:1 in XP‐variant. The strong tendency toward malignancy was seen only on the skin in both groups.
HYALURONIC ACID SYNTHETASE ACTIVITY IN LICHEN MYXEDEMATOSUS SKIN
Authors: Yamamoto Yasuo , Arata Jirô , Tokuyama Midori , Take Makoto , Nohara Nozomi 
Published: April 2015

ABSTRACTHyaluronic acid synthetase (HAS) activity of lyophilized skin from a 49‐year‐old Japanese man with lichen myxedematosus was assayed. The HAS activity was very high in both diseased and normal appearing skin.
A CASE OF MALIGNANT FIBROUS HISTIOCYTOMA
Authors: Sato Akihiko , Kato Taizo 
Published: April 2015

ABSTRACTA case of malignant fibrous histiocytoma is reported. Due to the histological features of cellular pleomorphism and myxoid degeneration seen also in other soft tissue sarcomas (rhabdomyosarcoma, liposarcoma), differential diagnosis was difficult. However, electron microscopic studies led to a distinct diagnosis of malignant fibrous histiocytoma.
XANTHOGRANULOMA IN THE ADULT
Authors: Nakamura Shinichi , Izawa Reiko , Nakayama Keiji , Hoshi Kenji , Onda Shutaro 
Published: April 2015

ABSTRACTA forty‐three year old man has had numerous papules on his face and neck for about two years without any systemic involvement. Some papules disappeared gradually within eight months without any therapy. Papules were examined with the light microscope and the electron microscope and histological findings were very similar to those of juvenile xanthogranuloma (JXG)1).
ECHOGRAPHIC EVALUATION OF NODULAR LESIONS OF THE SKIN
Authors: Miyauchi Shunji , Tada Masanori , Miki Yoshiharu 
Published: April 2015

ABSTRACTEchographic differentiation between clinically similar, nodular lesions of the dermis and subcutaneous fat was done in an attempt to open a new field of ultrasonic histopathology.Sector B‐mode echography with a 10 MHz transducer was done on 87 patients with nodular lesions in the dermis and subcutaneous fat, and the echograms were compared with microscopic findings. These consisted of nevus pigmentosus in 10, neurofibroma in 10, steatocystoma multiplex in 4, epidermoid cyst in 30, cavernous hemangioma in 4, lipoma in 10, panniculitis in 14 and malignant lymphoma in 5 patients.Neurofibroma and steatocystoma multiplex were as echolucent as lipoma, while nevus pigmentosus and epidermoid cyst were less echolucent. Small internal echoes were observed within the echolucent mass in 15 of 30 lesions of epidermoid cysts. Cavernous hemangioma, panniculitis, and malignant lymphoma were partially echogenic as well as echolucent.
UNILATERAL STEROID ACNE ON THE PARALYZED SIDE OF THE FACE
Authors: Tagami Hachiro 
Published: April 2015

ABSTRACTTherapy‐resistant acne developed about one month after the initiation of oral steroid therapy for Bell's palsy, chiefly on the paralyzed side of the face of a middle‐aged woman who had once suffered from acne vulgaris. It was speculated that the lack of normal facial movement contributed to the development of acne.
MODIFICATION OF HUMAN EPIDERMAL PREKERATIN IN THE REGION OF GRANULAR AND TRANSIENT CELL LAYERS
Authors: Toku Seikichi , Aso Kazuo , Katagata Yohtaro 
Published: April 2015

ABSTRACTTo investigate the process of keratin formation, changes in polypeptide composition of epidermal keratin and of prekeratin were examined using serial slices of human skin. In most of the skin surface, prekeratin was composed of 70K, 58K, 56K, 49.5K and occasionally 55K polypeptides, and an additional 63K polypeptide was present in the skin of the palm and sole. The decrease in molecular weight of these prekeratins to that of keratins was observed in the region of the granular and transient cell layers, especially in that of palm and sole. This was partially confirmed by preliminary digestion experiments in vitro. Our present studies suggest that limited proteolysis of higher molecular weight prekeratin(s) is a step in keratin formation of the stratum corneum.
IMMUNOLOGICAL STUDIES OF AN EXTRACELLULAR KERATINASE
Authors: Takiuchi Iwao , Higuchi Dousei , Sei Yoshihiro , Koga Miho 
Published: April 2015

ABSTRACTThe extracellular keratinase from M. canis was purified using ion exchange chromatography and gel filtration. The purified enzyme showed a single protein band on sodium dodecyl sulfate‐polyacrilamide gel electrophoresis. A molecular weight of approximately 45,000 was determined by SDS‐electrophoresis.Anti‐body directed against the purified enzyme from M. canis was obtained from New Zealand White rabbits, and the immunological identity of the enzyme from M. canis, as well as those from M. gypseum, T. mentagrophytes and T. rubrum, were demonstrated by immuno‐double‐diffusion experiments.
DEMONSTRATION OF IMMUNOGLOBULIN G IN NORMAL HUMAN EPIDERMIS BY LIGHT AND ELECTRON MICROSCOPY USING PEROXIDASE LABELED ANTIBODY
Authors: Yamada Mariko 
Published: April 2015

ABSTRACTDistribution of immunoglobulins in normal human epidermis was revealed at both the light and electron microscopic levels using peroxidase labeled antibodies. Biopsy specimens were obtained from various body locations of 40 healthy persons from 2 to 82 years old under local anesthesia. There were three types of IgG staining patterns in normal human epidermis. Intraepidermal IgG was found in about 80% of the skin samples. IgG was localized in the cytosol of squamous cells by electron microscopy. There were no morphological differences between the IgG positive and IgG negative cells. There seemed to be a correlation between IgG staining and the IgG serum level. The results of the present study suggest that with highly sensitive immunohistochemical techniques, IgG can be detected not only in diseased skin but also in normal skin.
PITYRIASIS PIGMENTOSA
Authors: Piamphongsant Thada 
Published: April 2015

ABSTRACTThis paper proposes a new skin disease entity—pityriasis pigmentosa. Characteristic lesions of this disease are well defined, oval or round, pigmented patches covered with fine, adherent, wrinkled scales. The size of the lesions varies from 0.5–3 cm in diameter. Some lesions gyrate or coalesce into larger patches. The lesions are typically located on the waist and the extremities and are particularly common on the volar aspect of the upper arms and the posterior aspect of the upper thighs. Using Wood's light examination, a bright golden fluorescence is inevitably seen. Rapid identification can be made by use of the KOH/Parker Ink preparation. This reveals characteristic branched, segmented hyphae and clusters of budding, oval‐shaped yeast cells. Pityrosporum ovale produces hyphae when cultured in glycine‐ammonium phosphate buffered media. Pityrosporum orbiculare does not produce hyphae by this method. These clinical and laboratory findings distinguish a clinical entity differing from Tinea (Pityriasis) versicolor which is caused by Pityrosporum orbiculare.
THE BROAD SPECTRUM OF DOWLING DEGOS DISEASE, INCLUDING HABER'S SYNDROME—A HEREDITARY ABNORMAL REACTIVITY TO STIMULATION, INCREASING WITH AGE?—CASE REPORTS AND MANAGEMENT
Authors: Kikuchi Ichiro , Inoue Shouhei , Narita Hiromi , Tada Shigeru , Yoshinaga Aiko , Amano Fukiko 
Published: April 2015

ABSTRACTFour cases of Dowling Degos disease (DDD) in one family and five cases of Haber's syndrome (HS) in two families were compared. DDD and HS share many symptoms and findings in common; histopathological features of downgrowths of epithelial cells, papules of either brown or natural skin color, and various kinds of skin pigmentation. Some had pitted scars, cysts, pruritus, telangiectasia, red cheeks, xerosis, and photosensitivity. Another feature common to both conditions is the progressive course of the disease; patients and their children should be followed over their lifetimes. One case of DDD had basal cell epithelioma within the area of reticular pigmentation, but an 83‐year‐old patient with DDD indicated that general health is not involved in DDD.One differentiating point for HS is that black warts in HS do not have the histologic features of DDD; they show only seborrheic keratosis. In HS, microscopic examinations should be made of as many varied lesions as possible.HS appears to be a subtype of DDD, characterized by red facies, seborrheic keratosis, and earlier onset than DDD. Both DDD and HS appear to be conditions with a different reactivity of the melanocyte‐keratinocyte system to external stimulation, rather than a disease of nevoid origin.
A CASE OF TUBEROUS SCLEROSIS WITH SUBCUTANEOUS NODULES
Authors: Shishiba Takako , Shimozuma Michiro 
Published: April 2015

ABSTRACTTuberous sclerosis (TS) is a well‐known clinical disease, characterized by epilepsy, mental retardation, adenoma sebaceum of Pringle, and various congenital anomalies. There have been a few reports of subcutaneous nodules as a cutaneous manifestation of TS. A 9‐year‐old Japanese boy who was diagnosed as having typical TS had one subcutaneous nodule on the breast and three on the back. Histologic examination of the breast nodule showed proliferation of homogeneous collagenous bundles encapsulated by thin connective tissue.
NATURAL KILLER CYTOTOXICITY OF HUMAN LYMPHOCYTES IN VITRO
Authors: Kondo Shigeo , Sato Kumi , Aso Kazuo 
Published: April 2015

ABSTRACTBCG, PHA, PWM, PPD and LPS‐Su all enhanced the natural killer‐like cytotoxicity of the nylon wool‐treated human lymphocyte fraction against a human skin squamous cell line (HSC‐1) in vitro. The cytotoxicity was measured by 51Cr release from the target cells (HSC‐1) when they were mixed with lymphocytes preincubated with or without the stimulators for three days. The activity of BCG and PPD in enhancing the lymphocyte cytotoxicity was stable after sonication and heating, although it was slightly lowered after treatment of BCG with streptomycin.The K‐562 cell line, which has been supposed to be specifically sensitive to the natural killer activity, proved to be also sensitive to the cytotoxic effect of the nylon wool‐treated lymphocyte fraction enhanced by BCG and PHA as well as OK‐432.
LABORATORY STUDIES OF COLLAGEN WOUND DRESSING (CAS)
Authors: Takeda Ueto , Izawa Masanori , Koeda Takemi , Shibata Uichi 
Published: April 2015

ABSTRACTThe efficacy of collagen wound dressing (CAS), a new biological dressing, was studied on experimental skin excisions and burns in rabbits, by comparing it with lyophilized porcine skin (LPS) and gauze dressings.CAS is a sterilized unwoven cloth made from fine spun fibres of bovine dermal atelocollagen which have been fixed with glutaraldehyde.In the tests, CAS was better than LPS and gauze in healing 20/1,000 inch thick skin excisions, deep excisions, and burns. CAS adhered to the wound surface well, allowing the exudate to be absorbed by the gauze on toop and be taken away from the wound surface. CAS broke down continuously with time to form pseudocrust and new tissue to help heal the wound.From these results, it can be concluded that CAS has good efficacy for skin excisions and burns.
SUPERFICIAL HYPERKERATOTIC CANDIDIASIS ON THE PALMS AND SOLES
Authors: Watanabe Shin Ichi , Shimozuma Michiro , Takizawa Kiyohiro 
Published: April 2015

ABSTRACTThis case report described a 59‐year‐old man with superficial hyperkeratotic candidiasis on the palms and soles. This rare form of cutaneous candidiasis is regarded as a complication of chronic mucocutaneous candidiasis or other immunodeficient disorders. However, the patient had no underlying disease and his cellular immunity was normal. The main abnormal findings in laboratory studies were markedly high IgE values and low vitamin A values. It was assumed that these played some role in the pathogenesis of the disease. The patient was cured through the oral administration of ketoconazole. Nine cases of superficial hyperkeratotic candidiasis on the palms have been reported in Japan, but the case given here is the first reported instance of this disease on the soles.
SKIN LESIONS ASSOCIATED WITH SJÖGREN'S SYNDROME AND ANTICYTOPLASMIC ANTIBODIES IN SLE PATIENTS
Authors: Miyagawa Sachiko , Kitamura Wataru , Sakamoto Kuniki 
Published: April 2015

ABSTRACTAmong forty‐two systemic lupus erythematosus patients, three had antibodies against cytoplasmic antigens SSA and SSB. All three patients showed annular nonscarring erythema as a sign of their illness. The most predominant site of skin lesions was the face, but trunk and extremities were involved as well. All of the patients had clinically distinct Sjögren's syndrome following the onset of their skin lesions.These findings suggest that systemic lupus erythematosus patients with anticytoplasmic antibodies might often have accompanying clinical features of nonscarring erythema and Sjögren's syndrome.
ULTRASTRUCTURES OF THE EPIDERMIS IN TINEA CRURIS
Authors: Sato Akihiko 
Published: April 2015

ABSTRACTElectron microscopy was used to study the epidermis in cases of tinea cruris due to infection by Trichophyton rubrum. Keratinocytes in the epidermis showed various degenerative features ranging from focal cell damage to whole cell necrosis. Two types of alterations, the diffuse loss of electron density in the cytoplasm and the reticular appearance of the cells, are presumably ultrastructural counterparts of intracellular edema. Intercellular edema appeared simultaneously with intracellular edema. Cells infiltrating the epidermis consisted mainly of neutrophilic leucocytes. There were no signs of close apposition of Langerhans cells to mononuclear cells.From these findings it is thought that alterations of the epidermis in tinea cruris can be classified as non‐specific degeneration possibly induced by toxic causes.Some information regarding the fine structures of hyphae and arthrospores living in the horny layer as parasites was also obtained.