P-512 Combined use of genetic diagnosis and algorithms for embryo quality assessment using artificial intelligence improves selection of embryos leading to clinical pregnancy
Authors: Pabón D , Perez M  D , Torres I , Lozano B , Cortés S , Peinado I , Navarro I , Bernad M  J , Gálvez J , Alvarez J , Pérez C 
Published: July 2024

Abstract

Study question
Analyze the results of our genetic diagnosis program, evaluating the morphokinetics of the embryo using an automated algorithm, based on its morphokinetic development and quality.

Summary answer
Transfers with a higher KIDScore D5 v3.2 euploid embryo have a significantly higher chance of achieving pregnancy.

What is known already
Embryo selection using morphokinetic algorithms is a technique that allows us to select embryos with better quality and perform more transfers of a single embryo improving the clinical outcomes of treatments, this technique combined with the study of aneuploidies and genetic diseases help us to increase the chances of success in embryo implantation and in the results of live newborn at home. Currently, there are techniques available for their diagnoses that, despite the impact of the processes, allow us to select euploid embryos with greater potential for implantation.

Study design, size, duration
Retrospective study between 1/5/2022 and 31/12/2023 of patients undergoing preimplantation genetic testing for aneuploidy (PGTA) and preimplantation genetic testing for monogenic diseases (PGTM). A single euploid embryo was transferred. Transferred embryos are compared according to KIDScore D5 v3.2.

Participants/materials, setting, methods
A total of 171 cycles have been performed with PGT, 97 patients have had at least one euploid embryo or transferable mosaic. The single embryos transferred euploids are compared according to the KIDScore D5 v3.2 automated system that ranks each embryo from one to ten, based on their morphokinetic development and quality. Implantation rate, clinical gestation rate and miscarriage are analyzed.

Main results and the role of chance
A total of 84 single embryo transfers were performed after PGT with an implantation rate of 60.71% and a clinical gestation rate per transfer of 52.38%. According to the morphokinetic KIDScore D5 v3.2 of the transferred euploid embryo, patients with negative pregnancy had a mean KIDScore D5 v3.2 of 6.5±1.3 and for patients with positive gestation it was 7.5±1.4 (S). The mean maternal age of patients with negative and positive pregnancy was 32.8±3.5 and 34.8±3.9, respectively (NS). For the clinical gestation rate, the mean KIDScore D5 v3.2 was 7.48±1.6 (S). The miscarriage rate was 7.84%.

Limitations, reasons for caution
The main challenges encompass genetic and reproductive counseling and the most efficient approach to blastocyst biopsy.

Wider implications of the findings
Our data suggest that transfers with a higher KIDScore D5v3.2 euploid embryo have a significantly higher probability of achieving pregnancy. The evolutionary gestation rate is significantly higher when the morphokinetics of the embryo are better. Further studies with a larger number of cases are needed to better assess this trend.

Trial registration number
not applicable. non-clinical trials