bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dopamine‐Modified Cationic Conjugated Polymer as a New Platform for pH Sensing and Autophagy Imaging
Authors: Wen Quanshan , Liu Libing , Yang Qiong , Lv Fengting , Wang Shu 
Published: September 2012

AbstractA dopamine‐modified conjugated polymer PFPDA is synthesized and characterized. At low pH, dopamine exists in its hydroquinone form and lacks the ability to quench fluorescence. At high pH, the proportion of the quinone form of dopamine increases due to its autooxidation, and efficient intramolecular electron transfer from the polymer main chain to quinone occurs, resulting in the quenching of the fluorescence of PFPDA. Thus, PFPDA exhibits a fluorescence “turn‐on” response at low pH. PFPDA possesses excellent photostability and exhibits no cytotoxicity, which makes it a good fluorescent material for pH sensing and cell imaging. A light‐induced hydroxyl anion emitter, MGCB, is also used to change the pH of the solution and thus regulate the fluorescence of PFPDA via remote control under light irradiation. Because the cytoplasm becomes acidic when cell autophagy occurs, PFPDA can also be used for autophagy imaging of HeLa cells with good selectivity.
Small and Stable Phosphorylcholine Zwitterionic Quantum Dots for Weak Nonspecific Phagocytosis and Effective Tat Peptide Functionalization
Authors: Liu Xiangsheng , Zhu Huiguang , Jin Qiao , Zhou Wenbo , Colvin Vicki L. , Ji Jian 
Published: September 2012

AbstractFacile surface modification of quantum dots (QDs) to make them water‐soluble, small, stable, antibiofouling, and functional is crucial for their biological applications. This study demonstrates a simple ligand‐exchange reaction to convert hydrophobic CdSe/ZnS QDs into water‐soluble QDs using amphiphilic, zwitterionic 11‐mercaptoundecylphosphorylcholine (HS‐PC). The phosphorylcholine (PC)‐modified QDs (QD‐PC) possess several advantages, such as small hydrodynamic diameter, good resistance to pH variations and high salinity, excellent stabiliy in 100% human plasma, and low protein adsorption. Importantly, the PC modification endows the QDs with very low, nonspecific interaction with cells, and strongly minimizes nonspecific phagocytosis of QDs by macrophages. In addition, cell penetrating Tat peptide functionalized QDs can be easily produced by mixing Tat with HS‐PC with various ratios, which is proved to effectively enhance QD ability to enter cells and accumulate around perinuclear region. Compared to traditional mercaptoundecanoic acid (MUA) modification, PC modification not only makes the cell penetrating QDs more stable and brighter, but also provides the Tat‐ and PC‐conjugated QDs with much lower nonspecific phagocytic uptake than the Tat‐ and MUA‐conjugated ones. This research will provide insights into designing suitable ligands for surface modification of QDs and improving biofunctional QD performance in biological applications.
Prevalence of low back symptoms and its consequences in relation to occupational group
Authors: Widanarko Baiduri , Legg Stephen , Stevenson Mark , Devereux Jason , Jones Geoff 
Published: September 2012

AbstractBackgroundThe purpose of this study was to examine: (1) the prevalence of low back symptoms (LBS) and its consequences (reduced activities and absenteeism); (2) the association between occupational group and LBS; and (3) the association between LBS and its consequences.MethodsA self‐administered questionnaire was used to determine the prevalence of LBS in 1,294 Indonesian coal mining workers. A Cox proportional hazards model was developed to quantify the 12‐monthly hazard of LBS. Logistic regression models were developed to identify risk factors for reduced activity and absenteeism from the workplace.ResultsThe 12‐month period prevalence for LBS, reduced activities, and absenteeism were 75%, 16%, and 13%, respectively. The 12‐monthly hazard of LBS for blue‐collar workers was 1.85 (95% CI: 1.06–3.25) times that of white‐collar workers. LBS and smoking increased the risk of reduced activity and absenteeism.ConclusionsIndonesian coal mining workers have a high prevalence of LBS. The findings imply that efforts to reduce LBS and in the workplace should focus on blue‐collar workers. For smokers who report reduced activities and/or absenteeism, there should be a focus on rehabilitation and/or return‐to‐work programs. Am. J. Ind. Med. 56:576–589, 2013. © 2012 Wiley Periodicals, Inc.
Re‐assigned diagnosis of D4ST1‐deficient Ehlers‐Danlos syndrome (adducted thumb‐clubfoot syndrome) after initial diagnosis of Marden‐Walker syndrome
Authors: Winters Kevin A. , Jiang Zhijie , Xu Weihong , Li Shibo , Ammous Zineb , Jayakar Parul , Wierenga Klaas J. 
Published: September 2012

AbstractWe report on a 16‐year‐old female originally diagnosed with Marden‐Walker syndrome due to features such as facial dysmorphism, several musculoskeletal anomalies, and atrial septal defect in addition to hypoplasia of the inferior vermis with normal‐sized cerebellum and absence of the septum pellucidum. However, an SNP array performed at age 15 years detected a total of 142 Mb of long runs of homozygosity (ROH), and put the diagnosis in doubt. Using the Genomic Oligoarray and SNP array evaluation tool (http://www.ccs.miami.edu/ROH), CHST14 provided a “hit” as a gene mapping to the largest ROH region associated with a phenotype matching our patient's (if mutated). At that time, she was a cognitively intact, thin female with growth parameters below the 3rd percentile. Craniofacial features included microcephaly, midface hypoplasia, blepharophimosis, entropion, myopia, microretrognathia, and dental malocclusion. Musculoskeletal features included kyphoscoliosis, arachnodactyly, camptodactyly, and rocker‐bottom feet with interphalangeal contractures. Her skin displayed large ecchymoses and poorly healed atrophic scars. Sequencing of CHST14 revealed a complex homozygous frameshift mutation involving a 7‐bp deletion and an 11‐bp insertion predicted to produce a truncated protein. This mutation was not seen in 100 controls of various ethnicities. Thus, our patient represents not only a novel (homozygous) mutation in CHST14, but is also the first patient with dermatan 4‐O‐sulfotransferase 1‐deficient Ehlers‐Danlos syndrome (adducted thumb‐clubfoot syndrome) (DD‐EDS ATCS) documented in the Western Hemisphere. Furthermore, our patient's central nervous system anomalies have not before been described in DD‐EDS (ATCS). © 2012 Wiley Periodicals, Inc.
Polymorphic haplotypes of CRELD1 differentially predispose Down syndrome and euploids individuals to atrioventricular septal defect
Authors: Ghosh Priyanka , Bhaumik Pranami , Ghosh Sujoy , Ozbek Umut , Feingold Eleanor , Maslen Cheryl , Sarkar Biswanath , Pramanik Vishmadeb , Biswas Priyanka , Bandyopadhyay Biswajit , Dey Subrata Kumar 
Published: September 2012

AbstractTo explore the role of CRELD1 variants on congenital heart defects, we sequenced the entire reading frame of CRELD1 in the samples from Kolkata and adjoining areas. Nearly, 400 participants were included in the genetic association study and they were stratified as Down syndrome (DS) with atrioventricular septal defect (AVSD), DS without AVSD, euploid with AVSD, and euploid without AVSD. A significant association was found between AVSD and three polymorphisms, namely rs9878047 (c.1049‐129T > C), rs3774207 (c.1119C > T), and rs73118372 (c.1136T > C) among the Down syndrome and euploid individuals. The polymorphism rs73118372, involves a transition (c.1136T > C) that leads to change in amino acid methionine to threonine which alters protein secondary structure as confirmed by the bioinformatics software SOPMA. In addition, two haplotypes, C‐T‐C and C‐T‐T, in the order of loci rs9878047‐rs3774207‐rs73118372 were associated with incidence of AVSD among euploid and Down syndrome, with a slightly higher odds ratio in the later group. We hypothesize that these haplotypes increase the risk of AVSD, and the susceptibility is exacerbated in DS, possibly due to the trisomy 21 genetic background. Moreover, we report for the first time on an interaction between the mutant alleles of rs3774207 and rs73118372 which could disrupt the delicate balance between different CRELD1 isoforms. © 2012 Wiley Periodicals, Inc.
An association and haplotype analysis of porcine maternal infanticide: A model for human puerperal psychosis?
Authors: Quilter C.R. , Sargent C.A. , Bauer J. , Bagga M. R. , Reiter C. P. , Hutchinson E. L. , Southwood O. I. , Evans G. , Mileham A. , Griffin D.K. , Affara N.A. 
Published: September 2012

AbstractAn association analysis using the Illumina porcine SNP60 beadchip was performed to identify SNPs significantly associated with porcine maternal infanticide. We previously hypothesised that this was a good animal model for human puerperal psychosis, an extreme form of postnatal mood disorder. Animals were selected from carefully phenotyped unrelated infanticide and control groups (representing extremes of the phenotypic spectrum), from four different lines. Permutation and sliding window analyses and an analysis to see which haplotypes were in linkage disequilibrium (LD) were compared to identify concordant regions. Across all analyses, intervals on SSCs 1, 3, 4, 10, and 13 were constant, contained genes associated with psychiatric or neurological disorders and were significant in multiple lines. The strongest (near GWS) consistent candidate region across all analyses and all breeds was the one located on SSC3 with one peak at 23.4 Mb, syntenic to a candidate region for bipolar disorder and another at 31.9 Mb, syntenic to a candidate region for human puerperal psychosis (16p13). From the haplotype/LD analysis, two regions reached genome wide significance (GWS): the first on SSC4 (KHDRBS3 to FAM135B), which was significant (−logP 5.57) in one Duroc based breed and is syntenic to a region in humans associated with cognition and neurotism; the second on SSC15, which was significant (−log10P 5.68) in two breeds and contained PAX3, which is expressed in the brain. © 2012 Wiley Periodicals, Inc.
Accuracy of intraoperative frozen margins for sinonasal malignancies and its implications for endoscopic resection of sinonasal melanomas
Authors: Chiu Alexander G. , Ma Yue 
Published: September 2012

BackgroundThe main objective of endoscopic tumor surgery remains similar to open approaches, with the goal being total tumor resection with clear margins. Beyond cosmesis, endoscopes offer the advantage of limiting the size of the resection as well as aiding in the procurement of tissue margins in areas adjacent to critical structures or deep in the sinonasal cavity. Because of the close proximity of these tumors to critical structures and classic otolaryngology teaching with the goal sparing normal sinonasal mucosa, sinonasal tumor resection margins tend to be more conservative than those practiced for the same type of tumor in a different anatomic location. What is not uniformly agreed upon is the optimal margin of resection as well as the reliability of intraoperative frozen margins for the varied histologic subtypes seen in sinonasal malignancies.MethodsRetrospective review of malignant sinonasal tumors resected endoscopically by 1 surgeon at 2 institutions between 2006 and 2011.ResultsThirty‐one patients with mixed histologies were identified, with the most common being mucosal melanoma (25.8%) and squamous cell carcinoma (23.3%). The overall false‐negative rate for intraoperative frozen margins was 6.5%, with both false negatives associated with mucosal melanoma. The false‐negative margin rate for mucosal melanoma was 25%.ConclusionIntraoperative frozen margins for sinonasal tumors are reliable for most histologic subtypes, with the exception of those for sinonasal mucosal melanomas. This has implications for the size of margins needed for the resection of sinonasal melanomas as they may need to be larger than those for other tumors.
Preparation of polysulfone microspheres with a hollow core/porous shell structure and their application for oil spill cleanup
Authors: Li Xianhua , Guo Yongcun , Zhang Jun , Zhang Lei 
Published: September 2012

AbstractPolysulfone (PSF) microspheres with a hollow core/porous shell structure were prepared by a water‐in‐oil‐in‐water emulsion solvent evaporation method. The morphology of PSF could be controlled by variation of the surfactants, which included oleic acid, poly(vinyl pyrrolidone), and tween 80. The three kinds of prepared microspheres were developed as sorbents for the selective removal of oil from water. PSF microspheres with a hollow core/porous shell structure exhibited the best separation efficiency, which was 44.8 times higher than that of the pristine PSF powder. The oil‐absorbed microspheres combined with unsinkability, appropriate size, and highly hydrophobic and superoleophilic properties could be quickly distributed and collected in seconds and exhibited recyclability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Structural changes in the carbon black network in carbon‐black‐filled styrene‐butadiene rubber samples cured with a two‐step process
Authors: Kato Atsushi , Isono Yoshinobu 
Published: September 2012

AbstractThe volume resistivity of carbon black (CB)‐filled crosslinked styrene‐butadiene rubber (SBR) samples cured in two steps and having a fixed elongation condition declined slightly when the elongation ratio (α) was 3 or smaller, whereas the value increased markedly at α = 4. Three‐dimensional transmission electron microscopy observations revealed that the closest interparticle distance between the carbon black aggregates (CBag's) and the CBag number density decreased at α = 1−2 but increased at α = 4 and that the distance between the centers of gravity of the closest CBag and the volume of CBag increased at α = 1−3 but decreased at α = 4. Also, the fraction of crosslinked chains of the carbon black network (CBnet) was decreased with elongation, and the fraction of branched chains increased. The fraction of isolated chains in particular showed a more pronounced increase at α > 3 compared with α < 3. These results presumably imply that for the sample with α > 3, the coarsened CBag's were finely divided and also that the CBnet was broken by elongation. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Facile covalent surface functionalization of multiwalled carbon nanotubes with poly(2‐hydroxyethyl methacrylate) and interface related studies when incorporated into epoxy composites
Authors: Curtzwiler Greg , Plagge Andreas , Vorst Keith , Story John 
Published: September 2012

AbstractCarbon nanotubes (CNTs) have seen increased interest from manufacturers as a nanofiber filler for the enhancement of various physical and mechanical properties. A major drawback for widespread commercial use has been the cost associated with growing, functionalizing, and incorporating CNTs into commercially available polymeric matrices. Accordingly, the main objective of this study was to investigate the effects of adding commercially viable functionalized multiwalled carbon nanotubes (MWCNT) to a commercially available epoxy matrix. The mechanical behavior of the nanocomposites was investigated by mechanical testing in tensile mode and fractures were examined by scanning electron microscopy. The thermal behavior was investigated by differential scanning calorimetry and thermogravimetric analysis. Molecular composition was analyzed by attenuated total reflectance Fourier transform infrared spectroscopy. Mechanical testing of the epoxy/functionalized‐MWCNT indicated that the 0.15 wt % functionalized MWCNT composite possessed the highest engineering stress and toughness out of the systems evaluated without affecting the Young's modulus of the material. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Decreasing but still high: bycatch of seabirds in gillnet fisheries along the German Baltic coast
Authors: Bellebaum Jochen , Schirmeister Bernd , Sonntag Nicole , Garthe Stefan 
Published: September 2012

ABSTRACT

The magnitude and temporal trends of seabird bycatch in coastal set net fisheries in the eastern part of the German Baltic Sea were studied, based on (i) a survey of 4% of the total fishery in the period 2006–2009, and (ii) results from bycatch monitoring in a part of this region covering a period of 20 years. Bird carcasses were collected and information on fishing effort obtained from fishermen using interviews and on‐board observations on selected trips.
Bycatch of seabirds occurred with all types of fishing gear and métiers studied, with highest bycatch rates in coastal lagoons. The minimum estimate of total bycatch in nets set by 440 commercial fishermen was found to be 17 551 (range 14 905–20 533) birds annually between November and May. Bycatch in set nets and on longlines in summer was much lower.
Bycatch rates were found to depend either directly on bird abundance as shown for the long‐tailed duck (Clangula hyemalis) or on predictors of abundance such as water depth and location. Bycatch in the Pomeranian Bay has decreased over 20 years due to the severe decline of seaducks, particularly of long‐tailed ducks, which were most frequently bycaught. The estimated individual bycatch risk has also decreased in long‐tailed ducks but the current monthly losses of 0.81% may still indicate a potential threat for this species.
Bycatch monitoring based on carcass collections and interviews proved to be feasible over a 20‐year period although the results contain some underreporting. Based on the results specific measures are recommended to reduce bycatch risk in the German coastal fisheries using targeted effort reductions and replacement of set nets with alternative gear. Copyright © 2012 John Wiley & Sons, Ltd.
Blocking of p38 and transforming growth factor β receptor pathways impairs the ability of tolerogenic dendritic cells to suppress murine arthritis
Authors: Gárate David , Rojas‐Colonelli Nicole , Peña Corina , Salazar Lorena , Abello Paula , Pesce Bárbara , Aravena Octavio , García‐González Paulina , Ribeiro Carolina H. , Molina María C. , Catalán Diego , Aguillón Juan C. 
Published: September 2012

AbstractObjectiveDendritic cells (DCs) modulated with lipopolysaccharide (LPS) are able to reduce inflammation when therapeutically administered into mice with collagen‐induced arthritis (CIA). The aim of this study was to uncover the mechanisms that define the tolerogenic effect of short‐term LPS‐modulated DCs on CIA.MethodsBone marrow–derived DCs were stimulated for 4 hours with LPS and characterized for their expression of maturation markers and their cytokine secretion profiles. Stimulated cells were treated with SB203580 or SB431542 to inhibit the p38 or transforming growth factor β (TGFβ) receptor pathway, respectively, or were left unmodified and, on day 35 after CIA induction, were used to inoculate mice. Disease severity was evaluated clinically. CD4+ T cell populations were counted in the spleen and lymph nodes from inoculated or untreated mice with CIA. CD4+ splenic T cells were transferred from mice with CIA treated with LPS‐stimulated DCs or from untreated mice with CIA into other mice with CIA on day 35 of arthritis.ResultsTreatment with LPS‐stimulated DCs increased the numbers of interleukin‐10 (IL‐10)–secreting and TGFβ‐secreting CD4+ T cells, but decreased the numbers of Th17 cells. Adoptive transfer of CD4+ T cells from treated mice with CIA reproduced the inhibition of active CIA accomplished with LPS‐stimulated DCs. The therapeutic effect of LPS‐stimulated DCs and their influence on T cell populations were abolished when the p38 and the TGFβ receptor pathways were inhibited.ConclusionDCs modulated short‐term (4 hours) with LPS are able to confer a sustained cure in mice with established arthritis by re‐educating the CD4+ T cell populations. This effect is dependent on the p38 and the TGFβ receptor signaling pathways, which suggests the participation of IL‐10 and TGFβ in the recovery of tolerance.
Determination of prostaglandin profiles in lipopolysaccharide‐challenged guinea pig spleen
Authors: Yao X. , Dai Y. , Johnson A. , Hass M. A. , Feleder C. 
Published: September 2012

ABSTRACTWe previously reported that splenic extract from lipopolysaccharide (LPS)‐challenged guinea pigs inhibits the exaggerated febrile response of splenectomized guinea pigs, suggesting that the spleen generates an inhibitory factor. Earlier results indicate that the factor is a lipid. In an effort to identify this factor, lipid fractions, isolated from splenic extracts of control and LPS‐challenged guinea pigs, were analyzed with emphasis on identifying and quantifying prostanoids, which according to current knowledge are the likely bioactive factors. Prostaglandins have been extensively implicated in central and peripheral thermoregulation, and thus these lipids were targeted for characterization in the spleen. Analysis was done on the splenic extracts using solid‐phase extraction, analytical and preparative thin‐layer chromatography (TLC) and high‐performance liquid chromatography–mass spectrometry (HPLC‐MS/MS). Four prostaglandins (PGs, 6‐keto‐PGF1α, PGF2α, PGE2 and PGD2) were identified and quantified. Our data shows that these PG levels are doubled in LPS‐treated guinea pig spleen compared with the control group. The methods used in this investigation to characterize PG in the spleen offer significant advantages over immunoassays previously used to identify and quantify PG in the spleen and other biological tissues. These methods will be utilized in further research needed to definitively characterize the role of splenic‐derived PG in modulation of the febrile response induced by LPS. Copyright © 2012 John Wiley & Sons, Ltd.
Convergence in Environmental Reporting: Assessing the Carbon Disclosure Project
Authors: Matisoff Daniel C. , Noonan Douglas S. , O'Brien John J. 
Published: September 2012

ABSTRACTWe perform content analysis on Carbon Disclosure Project (CDP) responses from 2003 to 2010, focusing on the extent to which firms account for indirect emissions and have exhibited convergence in carbon reporting. We also examine standardization in reporting and the variation of reporting behavior across industry and country. We find that the CDP has produced a mixed record of improved transparency. In some areas, such as Scope 2 emissions, the CDP has demonstrated an increase in transparency in later years. However, the transparency and quality of direct emissions and Scope 3 emissions have not improved over time. Japanese and European Union firms have increased transparency, while American firms have decreased transparency. Energy‐intensive industries have either increased transparency or remained the same, while less energy‐intensive industries have become less transparent. We demonstrate some evidence of a learning effect among firms after participating in the CDP survey. Copyright © 2012 John Wiley & Sons, Ltd and ERP Environment.