bluuebunny/crossref_metadata_2025_split huggingface dataset:
Convergent differential regulation of SLIT‐ROBO axon guidance genes in the brains of vocal learners
Authors: Wang Rui , Chen Chun‐Chun , Hara Erina , Rivas Miriam V. , Roulhac Petra L. , Howard Jason T. , Chakraborty Mukta , Audet Jean‐Nicolas , Jarvis Erich D. 
Published: November 2014

ABSTRACTOnly a few distantly related mammals and birds have the trait of complex vocal learning, which is the ability to imitate novel sounds. This ability is critical for speech acquisition and production in humans, and is attributed to specialized forebrain vocal control circuits that have several unique connections relative to adjacent brain circuits. As a result, it has been hypothesized that there could exist convergent changes in genes involved in neural connectivity of vocal learning circuits. In support of this hypothesis, expanding on our related study (Pfenning et al. [2014] Science 346: 1256846), here we show that the forebrain part of this circuit that makes a relatively rare direct connection to brainstem vocal motor neurons in independent lineages of vocal learning birds (songbird, parrot, and hummingbird) has specialized regulation of axon guidance genes from the SLIT–ROBO molecular pathway. The SLIT1 ligand was differentially downregulated in the motor song output nucleus that makes the direct projection, whereas its receptor ROBO1 was developmentally upregulated during critical periods for vocal learning. Vocal nonlearning bird species and male mice, which have much more limited vocal plasticity and associated circuits, did not show comparable specialized regulation of SLIT–ROBO genes in their nonvocal motor cortical regions. These findings are consistent with SLIT and ROBO gene dysfunctions associated with autism, dyslexia, and speech sound language disorders and suggest that convergent evolution of vocal learning was associated with convergent changes in the SLIT–ROBO axon guidance pathway. J. Comp. Neurol. 523:892–906, 2015. © 2014 Wiley Periodicals, Inc.
Perceived Parental Bonding, Early Maladaptive Schemas and Outcome in Schema Therapy of Cluster C Personality Problems
Authors: Hoffart Lunding Synve , Hoffart Asle 
Published: November 2014

ObjectiveThe objective of this paper was to examine the relationships between perceived parental bonding, Early Maladaptive Schemas (Young et al., 2003), and outcome of schema therapy of Cluster C personality problems and whether the perceptions of parental bonding could be influenced by schema therapy.MethodThe sample consisted of 45 patients with panic disorder and/or agoraphobia and Diagnostic and Statistical Manual of Mental Disorders, fourth edition, Cluster C personality traits who participated in an 11‐week inpatient programme consisting of two phases; the first was a 5‐week panic/agoraphobia‐focused cognitive therapy, whereas the second phase was a personality‐focused schema therapy. The patients were assessed at pre‐treatment, mid‐treatment and post‐treatment and at 1‐year follow‐up.ResultsOpposite to our hypothesis, lower paternal care at pre‐treatment was related to more reduction in Cluster C personality traits from pre‐treatment to 1‐year follow‐up. Maternal protection was related to the schema domains of impaired autonomy and exaggerated standards. Overall schema severity and the schema emotional inhibition at pre‐treatment were associated with less change in Cluster C traits. Perceived maternal care was reduced from pre‐treatment to 1‐year follow‐up, and more reduction in maternal care was related to less reduction in Cluster C traits.DiscussionParental bonding failed to predict treatment outcome in the expected direction, but maternal protection was related to two of the schema domains. Overall schema severity and the particular schema emotional inhibition predicted outcome. Furthermore, perceived maternal care was reduced from before to after treatment. Future studies should examine these questions in larger samples of Cluster C patients receiving schema therapy of a longer duration. Copyright © 2014 John Wiley & Sons, Ltd.Key Practitioner Message

Most schemas within the impaired autonomy domain and the schema self‐sacrifice seem to be related to low perceived maternal protection.
Overall schema severity and the schema emotional inhibition predict a poorer outcome of schema therapy of Cluster C personality problems, and therapy should give priority to emotional inhibition when this schema is endorsed.
Therapists should be aware that schema therapy carries the risk to lead to a more negative view of mother's care during upbringing and this risk is accentuated with less benefit of therapy.
Synergistic Effects of Mixing Sulfone and Ionic Liquid as Safe Electrolytes for Lithium Sulfur Batteries
Authors: Liao Chen , Guo Bingkun , Sun Xiao‐Guang , Dai Sheng 
Published: November 2014

AbstractA strategy of mixing both an ionic liquid and sulfone is reported to give synergistic effects of reducing viscosity, increasing ionic conductivity, reducing polysulfide dissolution, and improving safety. The mixtures of ionic liquids and sulfones also show distinctly different physicochemical properties, including thermal properties and crystallization behavior. By using these electrolytes, lithium sulfur batteries assembled with lithium and mesoporous carbon composites show a reversible specific capacity of 1265 mAh g−1 (second cycle) by using 40 % 1.0 M lithium bis(trifluoromethylsulfonyl)imide (LiTFSI) in N‐methyl‐N‐propylpyrrolidinium bis(trifluoromethylsulfonyl)imide with 60 % 1.0 M LiTFSI in methylisopropylsulfone in the first cycle. This capacity is slightly lower than that obtained in pure 1.0 M LiTFSI as the sulfone electrolyte; however, it exhibits excellent cycling stability and remains as high as 655 mAh g−1 even after 50 cycles. This strategy provides a method to alleviate polysulfide dissolution and redox shuttle phenomena, at the same time, with improved ionic conductivity.
Ferrocene and Cobaltocene Derivatives for Non‐Aqueous Redox Flow Batteries
Authors: Hwang Byunghyun , Park Min‐Sik , Kim Ketack 
Published: November 2014

AbstractFerrocene and cobaltocene and their derivatives are studied as new redox materials for redox flow cells. Their high reaction rates and moderate solubility are attractive properties for their use as active materials. The cyclability experiments are carried out in a static cell; the results showed that these materials exhibit stable capacity retention and predictable discharge potentials, which agree with the potential values from the cyclic voltammograms. The diffusion coefficients of these materials are 2 to 7 times higher than those of other non‐aqueous materials such as vanadium acetylacetonate, iron tris(2,2′‐bipyridine) complexes, and an organic benzene derivative.
Strategic management of transthoracic needle aspirates for histological subtyping and EGFR testing in patients with peripheral lung cancer: An institutional experience
Authors: Son Choonhee , Kang Eun‐Ju , Roh Mee Sook 
Published: November 2014

BackgroundLung cancer therapy is personalized based on the histological subtype and molecular status. Totally, 70% of lung cancer patients present in advanced stages and are diagnosed on small biopsy or cytology specimens, hence an accurate but tissue‐sparing approach is necessary. This study aimed to demonstrate efficient utilization of cell block (CB) on transthoracic needle aspiration (TTNA) for lung cancer subtyping, and to investigate the usefulness of needle washing after TTNA for assessing EGFR molecular status.MethodsEach TTNA specimen from the 79 peripheral lung masses was divided into three parts; liquid‐based cytology (LBC), CB (with or without immunohistochemistry), and needle washing for analysis of EGFR mutation using peptide nucleic acid‐mediated real‐time PCR clamping.ResultsTotally 79 specimens were diagnosed as malignancy, 75 (94.9%), benign, 3 (3.8%), and inadequate specimen, 1 (1.3%). The combination of LBC and CB (92.0%) showed a higher diagnostic yield for definitive subtyping of lung cancer than LBC alone (72.0%). Of the 75 malignant cases, 17 (22.7%) showed an EGFR mutation in needle washing specimens. EGFR mutational status was compared in all paired needle washing and scraped CBs with a 100% concordance.ConclusionsWe hereby proposed a strategy to maximize biological information retrieval from a limited TTNA specimen in patients with peripheral lung cancer. This algorithm indicated CB preparation for accurate histological subtyping and waste needle washing for molecular testing. Diagn. Cytopathol. 2015;43:532–538. © 2014 Wiley Periodicals, Inc.
A novel Wnt5a‐Frizzled4 signaling pathway mediates activity‐independent dendrite morphogenesis via the distal PDZ motif of Frizzled 4
Authors: Bian Wen‐Jie , Miao Wan‐Ying , He Shun‐Ji , Wan Zong‐Fang , Luo Zhen‐Ge , Yu Xiang 
Published: November 2014

ABSTRACTThe morphology of the dendritic tree is critical to neuronal function and neural circuit wiring. Several Wnt family members have been demonstrated to play important roles in dendrite development. However, the Wnt receptors responsible for mediating this process remain largely elusive. Using primary hippocampal neuronal cultures as a model system, we report that Frizzled4 (Fzd4), a member of the Fzd family of Wnt receptors, specifically signals downstream of Wnt5a to promote dendrite branching and growth. Interestingly, the less conserved distal PDZ binding motif of Fzd4, and not its conserved proximal Dvl‐interacting PDZ motif, is required for mediating this effect. We further showed that Dvl signaled parallel to and independent of Fzd4 in promoting dendrite growth. Unlike most previously described pathways, Wnt5a/Fzd4 signaling promoted dendrite development in an activity‐independent and autocrine fashion. Together, these results provide the first identification of a Wnt receptor for regulating dendrite development in the mammalian system, and demonstrate a novel function of the distal PDZ motif of Fzd4 in dendrite morphogenesis, thereby expanding our knowledge of the complex roles of Wnt signaling in neural development. © 2014 Wiley Periodicals, Inc. Develop Neurobiol 75: 805℃822, 2015
Control of Autonomous Mobile Robot Using Map Matching with Optimized Search Range
Authors: Komiya Kouhei , Miyashita Shunsuke , Maruoka Yutaka , Uchimura YUTAKA 
Published: November 2014

SUMMARYAccurate estimation of self‐position is indispensable for autonomous mobile robots. Map‐matching‐based self‐positioning is a promising method; however, adequate matching area selection is an issue to be solved. In this paper, we propose an adjustable map matching method that remedies a defect of the conventional template matching technology. The proposed method was evaluated experimentally, and the results confirmed the advantages of the proposed method.
An Organocatalytic Process for the Hydrolytic Cleavage of Dithianes Mediated by Imidazolium Ions: No Harsh Agents Required
Authors: Myles Lauren , Gathergood Nicholas , Connon Stephen J. 
Published: November 2014

AbstractA new, organocatalytic approach to the hydrolytic deprotection of dithianes has been developed involving a low‐toxicity imidazolium‐ion‐based catalyst in an aqueous medium. A complimentary solvent‐free method without added water and involving a sacrificial aldehyde is also reported. The catalyst does not appear to operate by a specific‐acid catalysis mechanism.
Functionalization of the meso‐Phenyl Ring of Rhodamine Dyes Through SNAr with Sulfur Nucleophiles: Synthesis, Biophysical Characterizations, and Comprehensive NMR Analysis
Authors: Mitronova Gyuzel Yu. , Polyakova Svetlana , Wurm Christian A. , Kolmakov Kirill , Wolfram Thomas , Meineke Dirk N. H. , Belov Vladimir N. , John Michael , Hell Stefan W. 
Published: November 2014

AbstractAromatic nucleophilic substitution (SNAr) of fluorine in 9‐(3′‐carboxy‐4′,5′,6′,7′‐tetrafluorophenyl) groups of xanthene dyes constitutes a powerful tool in dye design. Thiols and amines regioselectively replace F‐6′. This approach enables additional hydrophilic residues or functional groups required for bioconjugation to be introduced. By using this methodology, a “bright” and photostable dye for two‐color superresolution microscopy was synthesized (with absorption and emission maxima at 604 and 627 nm, respectively). In the case of red‐emitting rhodamine dyes with 3′‐carboxy‐4′,5′,7′‐trifluorophenyl residues, two‐dimensional NMR techniques and a chemical transformation were used to prove the precise position of the additional substituent – a carboxylic acid group linked through the S‐atom at C‐6′. Furthermore, simple 1H NMR spectra reliably permit the position of the additional carboxy substituent in the 3′‐carboxyphenyl ring (at C‐5′ or C‐6′) to be established. Information on the exact position of this substituent is significant for the design of molecular probes and for the prediction of the properties of their bioconjugates.
Fabrication of an Electrochemical E. coli Biosensor in Biowells Using Bimetallic Nanoparticle‐Labelled Antibodies
Authors: Eksi Haslet , Güzel Remziye , Güven Burcu , Boyaci Ismail Hakki , Solak Ali Osman 
Published: November 2014

AbstractAn electrochemical biosensor was developed for the determination of Escherichia coli (E. coli) in water. For this purpose, silver‐gold core‐shell (Ag@Au) bioconjugates and anti‐E. coli modified PS‐microwells were designed in a sandwich‐type format in order to obtain higher sensitivity and selectivity. Ag@Au bimetallic nanoparticles were synthesized by co‐reduction method. The core‐shell formation was analyzed by using UV‐Vis spectroscopy and transmission electron microscopy. Biotin labeled anti‐E. coli antibodies were coupled with Ag@Au nanoparticles to form bioconjugates. The electrochemical immunosensor was prepared by immobilizing anti‐E. coli on polystyrene (PS)‐microwells via chemical bonding. These modified microwells were identified with X‐ray photoelectron spectroscopy and surface enhanced Raman spectroscopy. E. coli was sandwiched between Ag@Au bioconjugates and anti‐E. coli on PS‐microwells at different concentrations. The relationship between the E. coli concentration and stripping current of gold ions (Au3+) were investigated by square wave anodic stripping voltammetry at pencil graphite electrode. The proposed method can provide some advantages such as lower detection limit and shorter detection time. The electrochemical response for the immunosensor was linear with the concentration of the E. coli in the range of 101 and 105 cfu/mL with a limit of detection 3 cfu/mL. The procedure maintains good sensitivity and repeatability and also offers utility in the fields of environmental monitoring and clinical diagnosis.
Where Would the EUR/CHF Exchange Rate be Without the SNB's Minimum Exchange Rate Policy?
Authors: Hanke Michael , Poulsen Rolf , Weissensteiner Alex 
Published: November 2014

AbstractSince its announcement made on September 6, 2011, the Swiss National Bank (SNB) has been pursuing the goal of a minimum EUR/CHF exchange rate of 1.20, promising to intervene on currency markets to prevent the exchange rate from falling below this level. We use a compound option pricing approach to estimate the latent exchange rate that would prevail in the absence of the SNB's interventions, together with the market's confidence in the SNB's commitment to this policy. © 2014 Wiley Periodicals, Inc. Jrl Fut Mark 35:1103–1116, 2015
Monte Carlo Simulation of the Neurofilament Brush
Authors: Jeong SeongMin , Zhou Xin , Zhulina Ekaterina B. , Jho YongSeok 
Published: November 2014

AbstractGrand canonical Monte Carlo (MC) simulations are performed to analyse conformations of projection domains in a neurofilament (NF) brush composed of three types of tails (L, M, and H) at different concentrations of added salt. The NF is modelled as a rigid, uniformly charged cylinder (backbone), surrounded by tethered, flexible polyampholytic chains with charge distributions and stoichiometry similar to those in natural NFs. We calculate the equilibrium trajectory for each projection; that is, the average distance of the amino acid residue from the surface of the backbone as a function of its ranking number. These trajectories demonstrate the tendency for projections to fold back and adopt loop‐like conformations at moderate solution salinities. We also perform extended analysis of MC data to identify metastable states of the system, and demonstrate representative snapshots of tail conformations in major metastable states.
A preliminary Study on the Improvement of the Ecological Corridor in Paddy Fields
Authors: Chen Rong‐Song , Wang Kuo‐Liang , Wu Chia‐Ying 
Published: November 2014

AbstractThe ecological engineering of rivers has been emphasized in recent years; however, scant attention has been focused on the ecological environments of paddy fields and irrigation canals. In this study, a paddy field in Yuanli Township, Miaoli County, Taiwan, was used as a test field. We improved the ecological corridor, ensuring the continuity of the irrigation canal and paddy field, thereby enabling a constant flow of water in the longitudinal section of the paddy field corridor to facilitate smooth upstream migration of fish. Fish could also migrate upstream in the lateral section of the paddy field. Subsequently, loach were used to assess the effect of an enhanced rock pile in a pool area of the simplified paddy fishway, and fish were allowed to migrate up and down the paddy field and irrigation canal. The results indicated that the difference in water levels (i.e. a bottleneck for creating an ecological corridor) was enhanced by the simplified paddy‐field fishway and enhanced rock pile. In addition, removing bottlenecks along the ecological corridor enabled fish and shrimp to migrate in the longitudinal and lateral directions. Copyright © 2014 John Wiley & Sons, Ltd.
Simultaneously enhanced production and molecular weight of pullulan using a two‐stage agitation speed control strategy
Authors: Wang Dahui , Bian Juanjuan , Wei Gongyuan , Jiang Min , Dong Mingsheng 
Published: November 2014

AbstractBACKGROUNDPullulan with high molecular weight (Mw) is attractive for industrial application. Previous studies indicated that high yield and high Mw of pullulan cannot be simultaneously obtained under constant agitation speeds or constant dissolved oxygen levels during batch pullulan fermentation. In this study, a two‐stage agitation speed control strategy was developed to obtain pullulan with both high yield and high Mw.RESULTSBatch pullulan production at various constant agitation speeds ranging from 200 to 600 rpm was carried out in a 5 L stirred tank fermentor. Based on kinetic analysis of batch processes, activity assays of the enzymes associated with pullulan biosynthesis and biodegradation, and intracellular energy supply under different constant agitation speeds, a two‐stage control strategy that shifted the agitation speed from 500 to 400 rpm at 18 h proved to effectively achieve simultaneous high yield of high Mw pullulan.CONCLUSIONA maximum pullulan concentration of 27.4 g L−1 and final Mw of 3.09 × 106 Da were obtained using the two‐stage agitation speed control strategy, which were both greatly improved compared with those obtained at constant agitation speeds. © 2014 Society of Chemical Industry
A Calderón‐preconditioned single source combined field integral equation for analysis of bi‐isotropic object
Authors: Shi Yan , Tian Cheng‐Yi , Liang Chang‐Hong 
Published: November 2014

AbstractIn this paper, a Calderón‐preconditoned single source combined field integral equation (SSCFIE) has been developed to analyze electromagnetic scattering from bi‐isotropic objects. The field decomposition method is adopted to split the homogeneous bi‐isotropic media into two uncoupled isotropic media instead of direct calculation of complicated Green's function in bi‐isotropic material. Unlike dual source integral equation, the Calderón‐preconditioned single source electric and magnetic integral equations in the presence of bi‐isotropic media are constructed and combined to make the proposed algorithm free from dense mesh breakdown and spurious resonance. Numerical results show good performance of the proposed algorithm. Copyright © 2014 John Wiley & Sons, Ltd.
Detection of fraudulent addition of bovine whey in water buffalo ricotta cheese by isoelectric focusing
Authors: Fuselli Fabio , Deluca Anna , Montepeloso Emanuela A , Ibba Giulia , Tidona Flavio , Longo Lucia , Marianella Rosa M 
Published: November 2014

AbstractBACKGROUNDPrevention of food fraud in the dairy field is a difficult issue for researchers, industries and policy makers, both for commercial and health reasons. Currently, no analytical method allows detection of the addition of bovine whey to water buffalo ricotta, so this fraudulent practice cannot be prevented. The authors' aim was to develop such a method.RESULTSThe conditions for extraction and purification of denatured ricotta whey proteins, which are unfolded and coagulated by heating during the production process, were optimized. The optimal composition of the polyacrylamide gel (pH range, type and concentration of chemical separator) was first evaluated and then the best conditions to perform the separation by isoelectric focusing were established. The performance of the method (precision, selectivity, robustness, sensibility) was determined.CONCLUSIONSThe method was shown to be reliable and robust for detection of the presence of bovine whey added to water buffalo Ricotta at percentages above 5% (v/v). The results suggest that the differences observed between bovine and water buffalo electrophoretic profiles are due to bovine β‐lactoglobulin isoform A, which is never detected in water buffalo samples. © 2014 Society of Chemical Industry
Intrinsic disorder mediates hepatitis C virus core–host cell protein interactions
Authors: Dolan Patrick T. , Roth Andrew P. , Xue Bin , Sun Ren , Dunker A. Keith , Uversky Vladimir N. , LaCount Douglas J. 
Published: November 2014

AbstractViral proteins bind to numerous cellular and viral proteins throughout the infection cycle. However, the mechanisms by which viral proteins interact with such large numbers of factors remain unknown. Cellular proteins that interact with multiple, distinct partners often do so through short sequences known as molecular recognition features (MoRFs) embedded within intrinsically disordered regions (IDRs). In this study, we report the first evidence that MoRFs in viral proteins play a similar role in targeting the host cell. Using a combination of evolutionary modeling, protein–protein interaction analyses and forward genetic screening, we systematically investigated two computationally predicted MoRFs within the N‐terminal IDR of the hepatitis C virus (HCV) Core protein. Sequence analysis of the MoRFs showed their conservation across all HCV genotypes and the canine and equine Hepaciviruses. Phylogenetic modeling indicated that the Core MoRFs are under stronger purifying selection than the surrounding sequence, suggesting that these modules have a biological function. Using the yeast two‐hybrid assay, we identified three cellular binding partners for each HCV Core MoRF, including two previously characterized cellular targets of HCV Core (DDX3X and NPM1). Random and site‐directed mutagenesis demonstrated that the predicted MoRF regions were required for binding to the cellular proteins, but that different residues within each MoRF were critical for binding to different partners. This study demonstrated that viruses may use intrinsic disorder to target multiple cellular proteins with the same amino acid sequence and provides a framework for characterizing the binding partners of other disordered regions in viral and cellular proteomes.