bluuebunny/crossref_metadata_2025_split huggingface dataset:
Properties of Partially Purified Mouse Epidermal Transglutaminase
Authors: Nakayama Juichiro , Osaki Mitsuhiko , Nagae Shonosuke , Asahi Masakazu , Urabe Harukuni 
Published: April 2015

AbstractMouse epidermal transglutaminase was partially purified from the epidermis of newborn BALB/c mice. The epidermal homogenate was subjected to CM‐cellulose chromatography and then to gel filtration on Sephadex G‐150. Two separate peaks with enzyme activity were reproducibly observed in CM‐cellulose chromatography. Gel filtration on Sephadex G‐150 of the peak fractions in CM‐cellulose chromatography gave a single enzyme activity peak (molecular weight about 55,000). Native polyacrylamide gel electrophoresis at pH 4.5 showed two major bands with enzyme activity. The final enzyme preparation (Sephadex G‐150 fraction) had a pH optimum of 10.0 in glycine‐NaOH buffer. Calcium ion and SH‐protecting agent (dithiothreitol) were essential for enzyme activity. The enzyme was not inactivated by heating at 56°C for 45 min in the presence of calcium. Trypsin treatment of the enzyme enhanced the activity about threefold; this enhancement was blocked by trypsin inhibitor. The enzyme bound to lysine‐Sepharose 4B in the presence of casein in Tris‐acetate buffer, pH 6.0, at 37°C; 40% of the enzyme activity was eluted from this enzyme‐lysine‐Sepharose complex with 1 M NaCl.
A Case of Poroma Folliculare on the Forearm
Authors: Muramatsu Tsutomu , Shirai Toshihiko , Sakamoto Kuniki , Umeda Sadamu 
Published: April 2015

AbstractA follicular tumor on the right forearm of a 25‐year‐old woman was reported. Histopathological examination revealed a pear‐shaped tumor mass with trichilemmal keratinization, extending from the surface epidermis into the deep dermis. The tumor was composed of glycogen‐laden cells with slightly clear cytoplasm and contained a hair shaft in the middle lower portion. Numerous squamous eddies were also observed. The histological findings from this tumor corresponded to those of poroma folliculare and inverted follicular keratosis.
Flow Cytometric Sorting and Keratin Analysis of Human Epidermal Basal Cells
Authors: Staquet Marie‐Jeanne , Lawrence Jean‐Jacques , Albert Jacques , Thivolet Jean 
Published: April 2015

AbstractA prerequisite condition for the elucidation of the expression of keratin subunits in human basal cells is obtaining highly purified basal cell fractions. A method has therefore been developed for the isolation of the basal cell sub‐population from normal human epidermis at very high purity. Epidermal cell suspensions obtained by trypsinization at 4°C were first enriched in basal cells by selective attachment to collagen. The cells were then stained in suspension with KLI monoclonal antikeratin antibody which had been shown to be reactive with suprabasal cells. The suprabasal cells contaminating the suspensions were then eliminated by selective flow cytometry sorting and basal keratinocyte populations of 99.5% purity were obtained. Analysis of the keratin composition of these cells showed that the large majority of basal cells contained two keratins, one from each keratin subfamily: type I acidic, 50 Kd [keratin no 14 as defined by Moll et al. (1)] and type II basic, 58 Kd [no 5]. Nevertheless, a small subpopulation of basal cells (2%) was shown to express, in addition to the 50/58 Kd subunits, the 56.5/65–67 Kd keratins (nos 10, 1–2) which are regarded as markers for cells committed to terminal differentiation.
A Time Course Study of the Proliferation of Sebaceous Glands Induced by Topically Applied Tetradecane in Rabbit Pinna Skin: Autoradiography and Electron Microscopy
Authors: Motoyoshi Katsuhiro , Ito Masaaki , Sakamoto Fumiko , Sato Yoshio 
Published: April 2015

AbstractTopically applied tetradecane is known to produce marked hyperplasia of sebaceous glands in the inner side skin of male rabbit pinnas. An autoradiographic and ultrastructural study was performed on these sebaceous glands to determine the changes in the proliferation activity of the peripheral sebaceous cells and their morphological changes.In a microautoradiographic study using 3H‐thymidine, the labeling index of the peripheral cells decreased at an early stage and thereafter rapidly increased up to ten times the normal level. The ultrastructural study of the treated pinnas showed a remarkable increase in the number of undifferentiated sebaceous cells. They were oval to cuboid in shape and formed several cell layers. Among these cells, electron dense sebaceous cells existed; this morphological change was already evident at hour (hr) 24 and gradually became remarkable with the time course.From these findings, it is clear that topically applied tetradecane may cause temporal damage to the peripheral sebaceous cells in the early stages and then active proliferation of these cells, probably by a stimulation of their DNA synthesis, results in production of a large number of sebaceous cells.
Comparison of the Nuclear Contour Index (NCI) and T cell Subsets of Erythematous, Plaque and Tumor Lesions in Patients with Mycosis Fungoides (MF)
Authors: Hantanyapong Yaowalak , Iwahara Kunio , Zhuan Kung Guo , Yaguchi Hitoshi , Nakajima Sumino , Ogawa Hideoki , Sei Yoshihiro , Takiuchi Iwao 
Published: April 2015

AbstractUsing monoclonal T cell antibodies, we compared T cell subsets of skin infiltrates with the nuclear contour index (NCI) of pan T cells of skin infiltrates from erythematous, plaque and tumor lesions in the same patients with mycosis fungoides (MF). In all lesions, the majority of the skin‐infiltrate population were T cells; helper T cells were especially dominant in the plaque and tumor lesions. The NCI of the T cells in skin infiltrates increased in parallel with advancing stages of the lesions in the same patient. The NCI data for T cell and T cell subsets in different stages of lesions in the same patient were similar to data from different stages of lesions in different patients. Therefore, even in tumor stage patients, topical treatment should be selected in accordance with the cytological stage of their lesions.
Multiple Linear Eccrine Spiradenoma Associated with Multiple Trichoepithelioma
Authors: Ikeya Toshihiko 
Published: April 2015

AbstractA 37‐year‐old woman had multiple trichoepithelioma of the face which showed typical clinical and histological features, together with multiple papular lesions of 30 years' duration. The lesions were arranged in a linear fashion on her right breast and right upper arm. Microscopic examination of a biopsy specimen revealed typical eccrine spiradenomas.An ultrastructural study of the tumor on the breast revealed that it was composed of two main types of cells: one was a clear cell with low‐density cytoplasm and the other was a dark cell with high‐density cytoplasm. Some of the clear cells had secretory granule‐like structures and/or tono‐fibrils, while others had intracytoplasmic cavitations. Neither myoepithelial cells nor Langerhans cells were observed.
Treatment of Psoriasis Vulgaris with Oral 1α,25‐Dihydroxyvitamin D3
Authors: Morimoto Shigeto , Yoshikawa Kunihiko , Kozuka Takehito , Kitano Yukio , Imanaka Shunji , Fukuo Keisuke , Koh Eio , Hironaka Takashi , Zen Akira , Nabata Takashi , Onishi Toshio , Kumahara Yuichi 
Published: April 2015

AbstractPsoriasis is a common inflammatory skin disease, but its pathogenesis is unknown. This paper presents 2 cases of psoriasis vulgaris in which the skin lesions were improved for 3 months and more after the start of oral treatment with 1α,25‐dihydroxyvitamin D3 at a dose of 0.5 μg/day. The circulating levels of calcium, inorganic phosphate, 25‐hydroxyvitamin D, 1α,25‐dihydroxyvitamin D, parathyroid hormone and calcitonin were within normal ranges before and after the start of treatment. These findings suggest that 1α,25‐dihydroxyvitamin D3 has a beneficial effect on psoriatic skin lesions. It probably has a direct effect on enhancing epidermal cell differentiation.Abbreviations: Ca: calcium, hCT: calcitonin, 25‐OHD: 25‐hydroxyvitamin D, 1,25‐(OH)2D: 1α,25‐dihydroxyvitamin D, 1,25‐(OH)2D3: 1α,25‐dihydroxyvitamin D3, Pi: inorganic phosphate
Disseminated Atypical Granuloma Annulare
Authors: Husz Sándor , Szabó Éva , Hunyadi János , Kohán József , Mónus Zoltán , Simon Nicolaus 
Published: April 2015

AbstractGeneralized granuloma annulare commonly occurs in older adults. Atypical (perforating) forms are well known, but treatment can sometimes be complicated. A case of atypical generalized granuloma annulare successfully treated with PUVA is reported. The histology and immunohistology of the skin, histopathologic, immunohistopathologic and electronmicroscopic studies of the liver, immunoserology and laboratory investigations are detailed. The most important findings were as follows; diabetic glucose tolerance test, typical histology of the skin disease, steatosis and non‐specific reactive hepatitis, focal Kupffer cell proliferation, Kupffer cell autoantibodies in the liver and slightly enhanced liver functions. The hepatic lesions observed and latent diabetes mellitus are considered to be correlated with the generalized dermal symptoms.
Reactive Perforating Collagenosis
Authors: Bhatia Krishan Kumar , Kumar Satish , Narang Anju 
Published: April 2015

AbstractA case of reactive perforating collagenosis (RPC) is presented. The lesions appeared in a female at the age of 4 years. So far, the patient has had only two episodes of RPC and both were precipitated by cold weather. The lesions showed the Koebner phenomenon. Histological sections showed the changes of “transepidermal elimination of necrobiotic connective tissue”.
Solitary Angiokeratoma Seen in a Patient with Pretibial Epidermolysis Bullosa
Authors: Maeda Manabu 
Published: April 2015

AbstractSolitary angiokeratoma was observed on the right leg of a 50‐year‐old Japanese female. In her childhood, she had repeatedly developed vesicles on the legs, and a possible diagnosis of pretibial epidermolysis bullosa was made, based on additional findings. Clinically, the condition resembled malignant melanoma and basal cell epithelioma. Possibly repeated injuries predisposed her to the development of solitary angiokeratoma.
Localization of Angiotensin Converting Enzyme (ACE) in Granulomas of Sarcoidosis Cutaneous Lesions
Authors: Takemura Satoshi , Fujioka Akira , Yasui Yumiko , Baba Shunichi , Suzuki Hiroyuki 
Published: April 2015

AbstractUsing an immunofluorescent technique, the localization of angiotensin converting enzyme (ACE) was investigated in granuloma lesions from the skin of three sarcoidosis cases. Specific fluorescence was observed in epithelioid cells in the sarcoidosis granulomas examined. However, it was not found in any other granulomatous diseases observed as controls, which included granuloma annulare, foreign body granuloma and lupus miliaris disseminatus faciei. These results indicate that ACE is specifically localized in the epithelioid cells in cutaneous granuloma lesions of sarcoidosis; in one case, it was also observed in Langhans giant cells. Therefore, it is suggested that examination for ACE in cutaneous lesions using an immunofluorescent technique is very useful for the diagnosis of sarcoidosis in the skin. We suggest that epithelioid cells of granulomas in sarcoidosis cutaneous lesions may play an important role in the increase of serum ACE activity.
Rabbit Zosteriform Eruption Induced by Intra‐arterial Inoculation of Herpes Simplex Virus, Type 1
Authors: Matsubara Motoo , Kishimoto Saburo , Yasuno Hirokazu , Sotomatsu Shigetaro , Yoshioka Akira , Tanaka Atsuo , Ueda Keiichi , Ohashi Masami 
Published: April 2015

AbstractSeventeen rabbits were inoculated with 0.1 ml of herpes simplex virus (HSV), type 1, Miyama strain (105,75 TCID50/0.1 ml), via the auricular artery; twelve of them developed zosteriform eruptions (ZE) on the skin. Seven rabbits treated with control medium showed no eruptions. This phenomenon was significant (P<0.01).ZE was observed about 7 days (mean time: 7.4 days) after inoculation. Although HSV antigen was found in dorsal root ganglia, spleen and liver by an immunofluorescence method, it was not detectable in the skin lesion. IgG, M and C3 were also undetectable in the skin lesion. On the other hand, complement requiring neutralizing antibody (CRN‐Ab) against HSV was detected on the 6th day after inoculation. Conventional neutralizing antibody was found on the 9th day after inoculation, but interferon could not be detected during the experimental period. The pathogenesis of ZE was discussed.
Studies of Blood Histamine Levels during Topical Corticosteroid Therapy Compared with Those during Systemically Administered Corticosteroids
Authors: Tanaka Keiko 
Published: April 2015

AbstractBlood histamine levels during topical corticosteroid therapy have not been reported, although decreased blood histamine levels are known to occur after the systemic administration of corticosteroid. Blood histamine levels and serum 11‐hydroxycorticosteroids (11‐OHCS) after systemic corticosteroid were compared with those after topical corticosteroid. Both blood histamine and serum 11‐OHCS levels decreased in a parallel and dose‐dependent manner during systemic administration of 0.5‐1.0 mg/day of betamethasone. Blood histamine levels increased and serum 11‐OHCS levels decreased during systemic administration of 0.25 mg/day to the betamethasone and topical corticosteroid group. Changes in blood histamine levels in the topical corticosteroid group appeared between 0.25 and 0.5 mg/day of systemically administered betamethasone.Fifteen cases of dermatological inpatients suffering from wide‐spread eczema or exfoliative dermatitis were treated with the topical corticosteroids betamethasone 17‐valerate, budesonide and diflorasone diacetate. In each case, blood histamine levels were examined and an equipotent dose of oral administeration of betamethasone was calculated. Thirteen cases in whom blood histamine levels were measured fell into the dosage range from 0.1 to 0.5 mg/day.In each case, serum 11‐OHCS levels were also examined and an equipotent dose of oral administration of betamethasone was calculated by the same method. Fourteen cases in whom serum 11‐OHCS levels were measured compared to a dose of less than 1.0 mg/day of betamethasone. Blood histamine levels in rats treated with 20 or 50 mg/kg/day of prednisolone were decreased significantly, and peripheral total leukocytes, lymphocytes, neutrophils and basophils were also decreased significantly.
KID Syndrome: Congenital Ichthyosiform Dermatosis with Keratitis and Deafness
Authors: Muramatsu Tsutomu , Shirai Toshihiko , Sakamoto Kuniki 
Published: April 2015

AbstractA 2‐year‐old male with KID syndrome was presented. Family history was negative for similar skin disease. The patient showed generalized ichthyosiform erythroderma, vascularizing keratitis, and neurosensory deafness. Additional clinical features included nail dystrophy, hypotrichosis, anhidrosis, and recurrent cutaneous bacterial and fungal infections. Histologic examination disclosed a basket‐weave pattern of hyperkeratosis and irregular papillomatous configuration of the epidermis. Electron microscopic examination showed an excessive amount of glycogen in the cytoplasm of erector pili muscle cells. This is the first case reported in Japan.
Cutaneous Invasion of Mucinous Adenocarcinoma of the Appendix
Authors: Nishitani Keiko , Nishitani Hiromu , Shimoda Yuichiro 
Published: April 2015

AbstractWe treated a patient with mucinous adenocarcinoma of the appendix with unusual cutaneous manifestation. As this entity is rare, it must be kept in mind when one sees a longstanding fistula of the abdominal wall in the region of an appendectomy scar.
Immunocyte Derived Amyloidosis
Authors: Rajasingam A.S. , Rrishnaram E.M. , Razack Abdul , Zahra Asfa 
Published: April 2015

AbstractA case of immunocyte derived amyloidosis (primary systemic amyloidosis) is being reported because of its rarity. The absence of petechiae and pinch purpura is particularly intriguing.
A Case of Trichilemmal Cyst on the Leg
Authors: Nakamura Shinichi , Nakayama Keiji , Ito Fumiyuki , Hoshi Kenji 
Published: April 2015

AbstractA 46‐year‐old man had a dome‐shaped tumor on his left leg without any involvement. Clinically, we considered epidermal cyst; however, histological features of the skin biopsy matched those of trichilemmal cyst
Multiple Ulcers with Heavy Colonization of Candida albicans on the Napkin Area of an Elderly Individual
Authors: Dekio Satoshi , Kawasaki Yohji , Jidoi Joji 
Published: April 2015

AbstractAn elderly diapered male with multiple ulcers on his diaper area is described. Candida albicans had colonized heavily on the ulcers. This condition is an unusual type of candidiasis of the diaper area of an elderly diapered individual.
Congenital Self‐healing Reticulohistiocytosis (Hashimoto‐Pritzker): A Case Report with a Solitary Lesion
Authors: Ofuji Shigeo , Tachibana Sawako , Kanato Mie , Horiguchi Yuji 
Published: April 2015

AbstractA small, dark‐reddish nodule noted on the left shoulder of a newborn baby showed thick proliferation of atypical histiocytic cells in the dermis with inflammatory infiltrating cells. Electron microscopy revealed the presence of Birbeck's granules in about one‐fourth of the histiocytic cells. At 36 days of age, the nodule disappeared spontaneously; no other skin lesions were observed throughout the course. This case was considered to be a solitary variant of congenital self‐healing reticulohistiocytosis (Hashimoto and Pritzker).
Radioimaging of Malignant Melanoma Xenografts in Nude Mice Using 111In‐labeled Monoclonal Antibody against Human Malignant Melanoma
Authors: Kanamaru Tetsuzan , Takezaki Shin‐ichiro , Nishiyama Shigeo , Ishii Katsumi 
Published: April 2015

AbstractBy using 111In‐labeled monoclonal antibody (ZME‐018) against human malignant melanoma, we examined its usefulness in radioimmunodetection of human melanoma xenografts in nude mice. Two human malignant melanoma cell lines were used in this study, KHm‐1/4; and KHm‐3/ps. KHm‐1/4 cells express melanoma‐associated antigen which is reactive with ZME‐018, whereas KHm‐3/ps cells do not. ZME‐018 was conjugated with DTPA (diethylene triamine pentaacetic acid) first; then the conjugate was labeled with 111In. After these procedures, labeled ZME‐018 retained its binding activity against KHm‐1/4 cells in vitro.Nude mice, bearing melenoma xenografts started from injections into the right hind legs, were i.p. or i.v. injected with ∽300 μCi (60 μg) of labeled ZME‐018. Tumor images were taken with gamma camera at 24 hr intervals. Clear tumor images were obtained by 24 hrs after injection. The best imaging was obtained at 72 hrs with no background of internal organs. Specific localization was confirmed by the absence of imaging in the mice which received free 111In only and also by using control mice bearing antigen‐negative melanoma cells (KHm‐3/PS). An example of tissue distribution of the labeled antibody in terms of tumor to tissue ratio at day 3 is as follows: tumor/intestine; 20/1, tumor/liver; 10/1, tumor/blood; 8/1, tumor/muscle; 8/1, tumor/heart; 3/1, tumor/kidney; 2/1.This study demonstrates the future applicability of 111In‐labeled monoclonal anti‐melanoma antibody for radioimmunodetection of metastatic lesions in melanoma patients.
Heterogeneity in Basement Membranes of Skin—Electrostatic Properties of Basal Lamina‐associated Anionic Sites
Authors: Kazama Takashi 
Published: April 2015

AbstractIn order to evaluate the charge‐selective filter function of basement membranes (BMs), electrostatic properties of anionic sites on the BMs of dermo‐epidermal junction were compared with those of the nuclear (the thick capillary wall) and the attenuated (the thin capillary wall) portions of capillary and other small blood vessels by determining salt concentrations required to remove ruthenium red from these BMs of the rat skin previously perfused with the cationic dye. The concentration of NaCl which was required to remove ruthenium red was more than 1.4M for the attenuated portion of capillary and only 1.2M for the other BMs, indicating that the former is more highly negatively charged than the latter. It is suggested that the BM of the attenuated portion of the capillary expedites the charge‐selective exchange of macromolecules more effectively than the other BMs.
Properdin Factor B Allotypes in Japanese Patients with Psoriasis
Authors: Dewald George , Nakagawa Hidemi , Tokunaga Katsushi , Ishibashi Yasumasa , Omoto Keiichi 
Published: April 2015

AbstractThe association between psoriasis and several antigens of the HLA system is well established. Factor B of the alternative pathway of complement activation is one of the complement components known to be controlled by a structural gene within the HLA chromosomal region. We investigated the genetic polymorphism of factor B (BF) in 69 Japanese psoriatic patients. By comparing the results with the BF phenotypes of 487 control subjects, we found significantly decreased frequencies of both the BF*F allele (0.01<p<0.025) and the BF FS phenotype in the patients (p=0.01). In individuals carrying the BF F gene product, the relative risk for developing psoriasis decreased to 0.43 (p<0.01). The BF*S allele and the homozygous SS genotype were significantly increased among psoriatic patients. These data suggest that the BF locus is associated with the HLA‐linked psoriasis susceptibility and/or resistance.
False‐positive Thormahlen Test Induced by Latamoxef Sodium
Authors: Murakami Shinji , Shiraishi Satoshi , Miyauchi Shunji , Miki Yoshiharu 
Published: April 2015

AbstractIntravenous administration of latamoxef sodium (LMOX) induced a false‐positive Thormählen test. When LMOX‐containing urine was alkalinized, the LMOX appeared to dissociate into N‐methyltetrazolthiol (N‐MTT) and a remainder. After subsequent purification of the Thormählen‐positive fraction with methyl ethyl ketone and twice repeated preparative thin‐layer chromatographies (PTLC), a crystalline residue was obtained. It was found to be almost identical to N‐MTT in high performance liquid chromatography (HPLC) pattern and 1H‐NMR analysis.
Dosage de la Fibronectine Plasmatique dans les Épidermolyses Bulleuses Congénitales
Authors: Imaizumi Takashi , Nomura Kazuo , Hashimoto Isao , Katabira Yasuo 
Published: April 2015

RésuméComme il est possible que la fibronectine (FN) ait un rapport avec la formation ou la guérison des bulles dans des cas d'épidermolyse bulleuse congénitale (É.B.C.), nous avons effectué les dosages de FN plasmatique dans 10 cas d'É.B.C. (3 É.B. simples, 2 É.B. dystrophiques dominantes, 5 É.B. dystrophiques recessives).Les taux de FN plasmatique ne sont pas fonction de l'âge et du sexe, mais ils sont en corrélation avec la gravité de la maladie. Résultats selon les types d'épidermolyses; pour celles de type simple, les taux se classent en trois groupes, très bas, bas, normal et leur moyenne est basse aussi, pour celles de type dominant, les taux sont presque normaux, pour celles de type récessif, les taux se classent en deux groupes, normal et bas. Tous les cas, où l'on rencontre des taux de FN bas, sont a l'état évolutif, et l'abaissement de la FN plasmatique est en corrélation inverse avec la gravité (p<0,01).
A Case of Cutaneous Involvement by Sinus Histiocytosis with Massive Lymphadenopathy
Authors: Aso Miki , Hagari Yoshitaka , Shimao Shuhei , Kimura Ryoichi , Shimizu Yasuyuki 
Published: April 2015

AbstractA 44‐year‐old woman developed an erythematous indurative plaque and papules on the right scapular region. Histological and ultrastructural findings were consistant with sinus histiocytosis with massive lymphadenopathy. However, lymphadenopathy was not demonstrated clinically and the lesion was limited to the skin.Immunohistochemical study showed that histiocytes were positive for S 100 protein and weakly positive to negative for lysozyme. We consider that the histiocytes in this cutaneous lesion of sinus histiocytosis with massive lymphadenopathy are “T‐zone histiocytes” and related to an immunoreaction to unknown antigens.