bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of testosterone on contractile properties of sexually dimorphic forelimb muscles in male bullfrogs (Rana catesbeiana, Shaw 1802)
Authors: Kampe Aaron R. , Peters Susan E. 
Published: July 2013

Summary
This study examined the effects of testosterone (T) on the contractile properties of two sexually dimorphic forelimb muscles and one non-dimorphic muscle in male bullfrogs (Rana catesbeiana, Shaw 1802). The dimorphic muscles in castrated males with testosterone replacement (T+) achieved higher forces and lower fatigability than did castrated males without replaced testosterone (T0 males), but the magnitude of the differences was low and many of the pair-wise comparisons of each muscle property were not statistically significant. However, when taken as a whole, the means of seven contractile properties varied in the directions expected of masculine values in T+ animals in the sexually dimorphic muscles. Moreover, these data, compared with previous data on male and female bullfrogs, show that values for T+ males are similar to normal males and are significantly different from females. The T0 males tended to be intermediate in character between T+ males and females, generally retaining masculine values. This suggests that the exposure of young males to T in their first breeding season produces a masculinizing effect on the sexually dimorphic muscles that is not reversed between breeding seasons when T levels are low. The relatively minor differences in contractile properties between T+ and T0 males may indicate that as circulating T levels rise during breeding season in normal males, contractile properties can be enhanced rapidly to maximal functional levels for breeding success.
Robust mitotic entry is ensured by a latching switch
Authors: Tuck Chloe , Zhang Tongli , Potapova Tamara , Malumbres Marcos , Novák Béla 
Published: July 2013

Summary
Cell cycle events are driven by Cyclin dependent kinases (CDKs) and by their counter-acting phosphatases. Activation of the Cdk1:Cyclin B complex during mitotic entry is controlled by the Wee1/Myt1 inhibitory kinases and by Cdc25 activatory phosphatase, which are themselves regulated by Cdk1:Cyclin B within two positive circuits. Impairing these two feedbacks with chemical inhibitors induces a transient entry into M phase referred to as mitotic collapse. The pathology of mitotic collapse reveals that the positive circuits play a significant role in maintaining the M phase state. To better understand the function of these feedback loops during G2/M transition, we propose a simple model for mitotic entry in mammalian cells including spatial control over Greatwall kinase phosphorylation. After parameter calibration, the model is able to recapture the complex and non-intuitive molecular dynamics reported by Potapova et al. (Potapova et al., 2011). Moreover, it predicts the temporal patterns of other mitotic regulators which have not yet been experimentally tested and suggests a general design principle of cell cycle control: latching switches buffer the cellular stresses which accompany cell cycle processes to ensure that the transitions are smooth and robust.
Nanometric Colloidal Sols of CeO2–ZrO2 Solid Solution as Catalyst Modifiers. I. Preparation and Structure
Authors: Tanimoto Kenji , Kato Hirokazu , Hidaka Miyuki , Hinokuma Satoshi , Ikeue Keita , Machida Masato 
Published: July 2013

Abstract
Stable nanometric colloidal sols consisting of fluorite-type metal oxides were prepared for use as chloride-free precursors for automotive catalysts. The aqueous solution containing metal carbonates and tetramethylammonium (TMA) ions was degassed and hydrothermally treated at 140–160 °C. Colloidal suspensions thus formed were purified by ultrafiltration to yield very stable and condensed sol solutions of CeO2 (CE), ZrO2 (ZR) and their solid solutions, CeO2–ZrO2 (CZ), which were stabilized by TMA. Each oxide sol contained 6–8 nm primary particles, but they formed aggregates larger than 40 nm. Local structural analysis by means of XAFS suggested that CZ sol exhibited two types of solid solution domains containing more or less Ce. The dried CZ sol exhibited higher thermal stability and oxygen storage capacity than CE and ZR sols.
Abnormally Large Thermal Vibration of Chloride Anions Incorporated in Layered Double Hydroxide Consisting of Mg and Al (Mg/Al = 2)
Authors: Sasai Ryo , Matsuoka Yoshiki , Sato Hiroaki , Moriyoshi Chikako , Kuroiwa Yoshihiro 
Published: July 2013

Abstract
Synchrotron-radiation X-ray diffraction enabled us to find the significantly large thermal vibration of chloride anions incorporated in a layered double hydroxide consisting of Mg and Al (Mg/Al = 2). The anomaly was ascribed to the hydrated water molecules in the interlayer.
Intrinsically Highly Hydrophobic Semi-alicyclic Fluorinated Polyimide Aerogel with Ultralow Dielectric Constants
Authors: Shen Dengxiong , Liu Jingang , Yang Haixia , Yang Shiyong 
Published: July 2013

Abstract
Polyimide aerogel with an intrinsically hydrophobic nature and a dielectric constant as low as 1.19 at a frequency of 2.75 GHz was synthesized by the polycondensation of an alicyclic dianhydride, an aromatic fluorinated diamine, and an amino-substituted polyhedral oligomeric silsesquioxane (POSS) end-capper, followed by a supercritical carbon dioxide drying procedure.
Synthesis, Characterization, and Evaluation of a Ferromagnetically Modified Natural Zeolite Composite for Removal Of Cs+ And Sr2+
Authors: Faghihian Hossein , Moayed Mohammad , Firooz Alireza , Iravani Mozhgan 
Published: July 2013

AbstractThe high fission yield and long half life of cesium and strontium make them the two most high-risk products from nuclear fission, so their separation from radioactive wastes is an important step in mitigating their harmful effects. Clinoptilolite, because of its thermal stability, high radiation resistance, and selectivity, was considered as the adsorbent for this purpose. In order to then separate the adsorbent-adsorbate complex from aqueous solution, the clinoptilolite was prepared as a magnetized composite with nanomagnetite. This magnetically modified zeolite enabled the efficient and quick separation of the adsorbent from solution using magnetic separation. The ability of this composite to remove Cs+ and Sr2+ from aqueous solutions was assessed and characterized using X-ray diffraction, X-ray fluorescence, Fourier-transform infrared spectroscopy, differential thermogravimetric analysis, and vibrating-sample magnetometry. Variables such as initial ion concentration, pH, contact time, and temperature in the sorption process were studied and optimized. The maximum adsorption capacities of the composite were 188.7 and 36.63 mg g-1 for Cs+ and Sr2+, respectively. Investigation of the kinetics revealed that the adsorption process onto the composite was quicker than in the case of the zeolite alone. The equilibrium data were analyzed using the Langmuir, Freundlich, and Dubinin-Radushkevich (D-R) isotherm models. The mean free energy of sorption (E) for both ions was in the range 8–16 kJ mol-1, confirming that an ion-exchange mechanism had occurred. Positive ΔH° and negative ΔG° values were indicative of the endothermic and spontaneous nature of the removal of Cs+ and Sr2+. The saturation magnetization of the composite was measured (17.46 Am2/kg), implying fast magnetic separation of the sample after adsorption. The results obtained revealed that the natural Iranian zeolite nanomagnetite composite was a good ion exchanger in the removal of Cs+ and Sr2+.
Temporal frames of reference
Authors: Evans Vyvyan 
Published: July 2013

AbstractBased on linguistic and behavioural evidence, representations for time appear to be structured in terms of space (e.g., Casasanto and Boroditsky 2008; Lakoff and Johnson 1980, 1999). This finding has led to a recent move to apply the theoretical construct of frames of reference (FoRs) from the domain of space to time, leading to sophisticated taxonomies for temporal frames of reference (e.g., Bender et al. 2010; Tenbrink 2011; Zinken 2010). The present paper argues that while space is important for modelling temporal reference, this is not the whole story. I argue that the experience types that in part underlie temporal representations are inherently temporal, rather than spatial in nature. They consist of a range of experience types, the hallmark of FoRs in the domain of time being transience (Galton 2011), a construct worked out in some detail. The present paper proposes three distinct types of temporal frames of reference (t-FoRs), anchored to three distinct types of transience. These proposals are argued to complement and enhance existing proposals for t-FoRs, rather than replacing them.
What is a domain? Dimensional structures versus meronomic relations
Authors: Gärdenfors Peter , Löhndorf Simone 
Published: July 2013

AbstractWithin cognitive linguistics, the notion of domain is central. In the literature, the notion of domain has been interpreted in an all-encompassing way, which has led to conceptual confusion. The article proposes to distinguish between a more psychologically oriented description of domains based on dimensional structures, on the one hand; and meronomic relations, on the other. It is shown how Langacker's notion of a configurational domain can be analyzed as higher-level dimensional structures. An added benefit of the distinction between dimensional domains and meronomic relations is that it generates a natural account of the difference between metaphors and metonymies.
Collostructional analysis and other ways of measuring lexicogrammatical attraction: Theoretical premises, practical problems and cognitive underpinnings
Authors: Schmid Hans-Jörg , Küchenhoff Helmut 
Published: July 2013

AbstractCollostructional analysis is a corpus-based quantitative method of measuring the mutual attraction of lexemes and constructions (cf. Stefanowitsch and Gries 2003) which has gained considerable popularity among corpus linguists and especially cognitive linguists with a statistical bent. For many less statistically minded linguists, it has proven rather difficult to evaluate the theoretical background assumptions and cognitive underpinnings of collostructional analysis and to compare them to alternative ways of modelling lexicogrammatical attraction phenomena. This paper aims to spell out these premises and foundations in terms comprehensible to a wider audience. It begins with a concise survey of how collostructional analysis works and then reports on a number of practical, theoretical and statistical issues of which both practitioners of the method and those who try to appreciate results of its application should be aware. With these issues in mind we then discuss alternative ways of calculating and interpreting lexicogrammatical attraction. The advantages and disadvantages of the different methods are discussed, also against the background of the results of studies that have tried to evaluate the measures by means of external evidence from psycholinguistic experiments. Finally, cognitive underpinnings of lexicogrammatical associations and implications for the different approaches are discussed. It is argued that at present we lack adequate knowledge about the ways in which discourse frequencies affect entrenchment. We conclude that the complexities of the relation between corpus frequencies and degrees of entrenchment are still rather poorly understood, and make suggestions for future work.
Alternative Monotonicity Assumptions for Improving Bounds on Natural Direct Effects
Authors: Chiba Yasutaka , Taguri Masataka 
Published: July 2013

AbstractEstimating the direct effect of a treatment on an outcome is often the focus of epidemiological and clinical research, when the treatment has more than one specified pathway to the defined outcome. Even if the total effect is unconfounded, the direct effect is not identified when unmeasured variables affect the intermediate and outcome variables. Therefore, bounds on direct effects have been presented via linear programming under two common definitions of direct effects: controlled and natural. Here, we propose bounds on natural direct effects without using linear programming, because such bounds on controlled direct effects have already been proposed. To derive narrow bounds, we introduce two monotonicity assumptions that are weaker than those in previous studies and another monotonicity assumption. Furthermore, we do not assume that an outcome variable is binary, whereas previous studies have made that assumption. An additional advantage of our bounds is that the bounding formulas are extremely simple. The proposed bounds are illustrated using a randomized trial for coronary heart disease.
Characterization of the myometrial transcriptome in women with an arrest of dilatation during labor
Authors: Chaemsaithong Piya , Madan Ichchha , Romero Roberto , Than Nandor Gabor , Tarca Adi L. , Draghici Sorin , Bhatti Gaurav , Yeo Lami , Mazor Moshe , Kim Chong Jai , Hassan Sonia S. , Chaiworapongsa Tinnakorn 
Published: July 2013

Abstract

Objective: The molecular basis of failure to progress in labor is poorly understood. This study was undertaken to characterize the myometrial transcriptome of patients with an arrest of dilatation (AODIL).

Study design: Human myometrium was prospectively collected from women in the following groups: (1) spontaneous term labor (TL; n=29) and (2) arrest of dilatation (AODIL; n=14). Gene expression was characterized using Illumina® HumanHT-12 microarrays. A moderated Student’s t-test and false discovery rate adjustment were used for analysis. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) of selected genes was performed in an independent sample set. Pathway analysis was performed on the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database using Pathway Analysis with Down-weighting of Overlapping Genes (PADOG). The MetaCore knowledge base was also searched for pathway analysis.

Results: (1) Forty-two differentially expressed genes were identified in women with an AODIL; (2) gene ontology analysis indicated enrichment of biological processes, which included regulation of angiogenesis, response to hypoxia, inflammatory response, and chemokine-mediated signaling pathway. Enriched molecular functions included transcription repressor activity, heat shock protein (Hsp) 90 binding, and nitric oxide synthase (NOS) activity; (3) MetaCore analysis identified immune response chemokine (C-C motif) ligand 2 (CCL2) signaling, muscle contraction regulation of endothelial nitric oxide synthase (eNOS) activity in endothelial cells, and triiodothyronine and thyroxine signaling as significantly overrepresented (false discovery rate <0.05); (4) qRT-PCR confirmed the overexpression of Nitric oxide synthase 3 (NOS3); hypoxic ischemic factor 1A (HIF1A); Chemokine (C-C motif) ligand 2 (CCL2); angiopoietin-like 4 (ANGPTL4); ADAM metallopeptidase with thrombospondin type 1, motif 9 (ADAMTS9); G protein-coupled receptor 4 (GPR4); metallothionein 1A (MT1A); MT2A; and selectin E (SELE) in an AODIL.

Conclusion: The myometrium of women with AODIL has a stereotypic transcriptome profile. This disorder has been associated with a pattern of gene expression involved in muscle contraction, an inflammatory response, and hypoxia. This is the first comprehensive and unbiased examination of the molecular basis of an AODIL.
snow white, a Zebrafish Model of Hermansky-Pudlak Syndrome Type 5
Authors: Daly Christina M S , Willer Jason , Gregg Ronald , Gross Jeffrey M 
Published: July 2013

Abstract
Hermansky-Pudlak Syndrome (HPS) is a set of genetically heterogeneous diseases caused by mutations in one of nine known HPS genes. HPS patients display oculocutaneous hypopigmentation and bleeding diathesis and, depending on the disease subtype, pulmonary fibrosis, congenital nystagmus, reduced visual acuity, and platelet aggregation deficiency. Mouse models for all known HPS subtypes have contributed greatly to our understanding of the disease, but many of the molecular and cellular mechanisms underlying HPS remain unknown. Here, we characterize ocular defects in the zebrafish (Danio rerio) mutant snow white (snw), which possesses a recessive, missense mutation in hps5 (hps5I76N). Melanosome biogenesis is disrupted in snw/hps5 mutants, resulting in hypopigmentation, a significant decrease in the number, size, and maturity of melanosomes, and the presence of ectopic multi-melanosome clusters throughout the mutant retina and choroid. snw/hps5I76N is the first Hps5 mutation identified within the N-terminal WD40 repeat protein–protein binding domain. Through in vitro coexpression assays, we demonstrate that Hps5I76N retains the ability to bind its protein complex partners, Hps3 and Hps6. Furthermore, while Hps5 and Hps6 stabilize each other’s expression, this stabilization is disrupted by Hps5I76N. The snw/hps5I76N mutant provides a valuable resource for structure–function analyses of Hps5 and enables further elucidation of the molecular and cellular mechanisms underlying HPS.
Traits of a good ecologist: What do ecologists think?
Authors: Reiners William A. , Reiners Derek S. , Lockwood Jeffrey A. 
Published: July 2013

Much is inferred, but little is actually known, about how ecologists view themselves and what they value as scientists. We investigated these attitudes as part of a survey of non‐student, U.S.‐addressed members of the Ecological Society of America. The part of the survey reported in this paper requested ratings of 15 possible traits of a good ecologist. These traits embodied values associated with religious principles, aesthetics, caring for nature, and epistemic competence (via professional proficiency). The survey was completed by 20% of the potential respondent pool of 6,083 ecologists. The resulting trait scores were analyzed with respect to age, gender, level of education, nature of employment, primary domain of inquiry, principle method of analysis, and source of greatest professional satisfaction.Respondents ranked traits associated with professional proficiency highest and religious principles lowest. Reactions to ethical and aesthetic traits were mixed. With increasing age, rankings for ethical and aesthetic traits increased while scores for some aspects of epistemic proficiency declined. Gender effects were few. Ecologists with BA/BS or MA/MS degrees scored aesthetic and pro‐social traits higher and epistemic proficiency traits lower than did PhD degree‐holding respondents. Ecologists employed by government, business, or non‐profits had uniquely different scoring spectra from one another and from the entire pool. Characterization of values held by ecologists classified by various domains of inquiry, primary methods of analysis, or sources of greatest professional satisfaction was complex. Particularly intriguing was that those who worked in the ecosystem domain, who used modeling and meta‐data analysis as methods, or who received the most professional satisfaction from these same activities, exhibited strong support for several epistemic proficiency‐related traits, while being relatively negative about traits in the aesthetic and nature‐caring categories. These results put some empirical substance and conceptual structures behind perceptions that distinct subcultures exist in U.S. ecology.These results are relevant in three ways. First, they provide a framework by which individual ecologists may become aware of the motivations that drive them, the values that shape their attitudes, and the source and meaning of their professional priorities. Second, these results challenge mentors to be mindful of how they guide young ecologists in the development of their own values and priorities. Third, these results inform the leadership of professional organizations of the range and variation of positions that their member hold and that are relevant to initiatives the leadership may which to promote.
The analysis of the flow of a micropolar fluid between two orthogonally moving porous disks with counter rotating directions
Authors: Sun Yina , Si Xinhui , Zheng Liancun , Shen Yanan , Zhang Xinxin 
Published: July 2013

Abstract
The present work investigates the unsteady, imcompressible flow of a micropolar fluid between two orthogonally moving porous coaxial disks. The lower and upper disks are rotating with the same angular speed in counter directions. The flows are driven by the contraction and the rotation of the disks. An extension of the Von Kármán type similarity transformation is proposed and is applied to reduce the governing partial differential equations (PDEs) to a set of non-linear coupled ordinary differential equations (ODEs) in dimensionless form. These differential equations with appropriate boundary conditions are responsible for the flow behavior between large but finite coaxial rotating disks. The analytical solutions are obtained by employing the homotopy analysis method. The effects of some various physical parameters like the expansion ratio, the rotational Reynolds number, the permeability Reynolds number, and micropolar parameters on the velocity fields are observed in graphs and discussed in detail.
The NTPase/helicase domain of hepatitis C virus nonstructural protein 3 inhibits protein kinase C independently of its NTPase activity
Authors: Hartjen Philip , Höchst Bastian , Heim Denise , Kammer Henning , Lucke Judith , Reinholz Michael , Baier Andrea , Smeets Ralf , Wege Henning , Borowski Peter , Wiesch Julian 
Published: July 2013

AbstractHelicase motif VI is a short arginine-rich motif within the NTPase/helicase domain of the non-structural protein 3 (NS3) of the hepatitis C virus (HCV). We previously demonstrated that it reduces the catalytic activity and intracellular shuttling of protein kinase C (PKC). Thus, NS3-mediated PKC inhibition may be involved in HCV-associated hepatocellular carcinoma (HCC). In this study, we expand on our earlier results, which were obtained in experiments with short fragments of NS3, to show for the first time that the catalytically active, longer C-terminal NTPase/helicase of NS3 acts as a potent PKC inhibitor in vitro. PKC inhibition assays with the NTPase-inactive mutant NS3h-D1316A revealed a mixed type kinetic inhibition pattern. A broad range of 11 PKC isotypes was tested and all of the PKC isotypes were inhibited with IC50-values in the low micromolar range. These findings were confirmed for the wild-type NTPase/helicase domain in a non-radiometric PKC inhibition assay with ATP regeneration to rule out any effect of ATP hydrolysis caused by its NTPase activity. PKCα was inhibited with a micromolar IC50 in this assay, which compares well with our result for NS3h-D1316A (IC50 = 0.7 μM). In summary, these results confirm that catalytically active NS3 NTPase/helicase can act in an analogous manner to shorter NS3 fragments as a pseudosubstrate inhibitor of PKC.