bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesoscale air transport at a midlatitude squall line in Europe – a numerical analysis
Authors: Uebel M. , Bott A. 
Published: June 2015

This study examines a fast‐propagating squall line over Western and Central Europe. A high‐resolution regional weather prediction model is used to analyze the three‐dimensional wind field and the corresponding air mass transport induced by the mesoscale convective system. The squall line can be divided into a bow‐shaped strong part with a continuous line of heavy convective precipitation, and a weaker part exhibiting several isolated multi‐cell storms. In the strong part, a deep and intense rear inflow jet occurs with relative wind speeds of 12–18 m s−1. This part is also characterized by moderate to strong wind shear, in contrast to the weaker part of the squall line where a shallow cold pool surges under the potentially unstable presquall air.To investigate the air mass transport along the squall line, in the model simulations passive fluid tracers are initialized in several vertical layers with different thermodynamic conditions. At the strong part of the squall line a very efficient vertical tracer transport occurs. Potentially warm low‐level air is lifted from the lowermost 2 km to the upper troposphere where an organized horizontal outflow is observed. The potentially coldest presquall air descends just in front of the updraught region and partly penetrates the convective precipitation area. In the weaker part of the squall line, the potential instability is not significantly reduced because the vertical air mass transport is less effective there. Additionally, passive tracers are used to analyze the composition of the cold pool which turns out to be a very heterogeneous mixture of descending post‐frontal cold air and presquall air originating at different heights.
How humans evolved according to grade 12 students in Singapore
Authors: Seoh Kah Huat Robin , Subramaniam R. , Hoh Yin Kiong 
Published: June 2015

AbstractTree thinking, the understanding of the evolutionary relationships between organisms depicted in different types of tree diagrams, is an integral part of understanding evolution. Novice learners often read tree diagrams differently from specialists, resulting in diverse interpretations of the relationships depicted. The aim of this study is to understand how learners construct the picture of human phylogeny, focusing particularly on their views of the last common ancestor of two sister species, humans, and chimpanzees. A theoretical framework based primarily on the schema theory is used to interpret these views. The primary study gathered responses to an open‐ended question about human ancestry at various time points in our evolutionary history. The data were coded into categories consisting of lineage, trait description, trait gain, trait loss, and others. The lineage responses were further categorized and examined in the secondary study, with the provision of a simplified phylogenetic tree and the main lineage responses obtained in the primary study. Thirteen students were also selected for interviews. Diverse interpretations, including those that consider humans as direct descendants of extant apes (transmutation) or products of hybridization between extant apes, were found to be affected by respondents' alternative schemata related to the last common ancestor and deep time. We also discuss the potential influence of religious and cultural worldviews on some of these interpretations and present nine alternative models of how learners visualize the last common ancestor that humans shared with the chimpanzee. Findings suggest that complex interactions between different sets of schemata (consilience) may result in diverse interpretations of simple phylogenetic trees. Implications for the teaching of tree thinking are provided. © 2015 Wiley Periodicals, Inc. J Res Sci Teach 53: 291–323, 2016.
Efficient production of indigoidine in Escherichia coli
Authors: Xu Fuchao , Gage David , Zhan Jixun 
Published: June 2015

Abstract
Indigoidine is a bacterial natural product with antioxidant and antimicrobial activities. Its bright blue color resembles the industrial dye indigo, thus representing a new natural blue dye that may find uses in industry. In our previous study, an indigoidine synthetase Sc-IndC and an associated helper protein Sc-IndB were identified from Streptomyces chromofuscus ATCC 49982 and successfully expressed in Escherichia coli BAP1 to produce the blue pigment at 3.93 g/l. To further improve the production of indigoidine, in this work, the direct biosynthetic precursor l-glutamine was fed into the fermentation broth of the engineered E. coli strain harboring Sc-IndC and Sc-IndB. The highest titer of indigoidine reached 8.81 ± 0.21 g/l at 1.46 g/l l-glutamine. Given the relatively high price of l-glutamine, a metabolic engineering technique was used to directly enhance the in situ supply of this precursor. A glutamine synthetase gene (glnA) was amplified from E. coli and co-expressed with Sc-indC and Sc-indB in E. coli BAP1, leading to the production of indigoidine at 5.75 ± 0.09 g/l. Because a nitrogen source is required for amino acid biosynthesis, we then tested the effect of different nitrogen-containing salts on the supply of l-glutamine and subsequent indigoidine production. Among the four tested salts including (NH4)2SO4, NH4Cl, (NH4)2HPO4 and KNO3, (NH4)2HPO4 showed the best effect on improving the titer of indigoidine. Different concentrations of (NH4)2HPO4 were added to the fermentation broths of E. coli BAP1/Sc-IndC+Sc-IndB+GlnA, and the titer reached the highest (7.08 ± 0.11 g/l) at 2.5 mM (NH4)2HPO4. This work provides two efficient methods for the production of this promising blue pigment in E. coli.
Alternative nighttime nutrition regimens in glycogen storage disease type I: a controlled crossover study
Authors: Hochuli Michel , Christ Emanuel , Meienberg Fabian , Lehmann Roger , Krützfeldt Jan , Baumgartner Matthias R. 
Published: June 2015

AbstractBackgroundTraditional approaches for nighttime glycemic control in glycogen storage disease type I (GSDI) include continuous tube feeding, or ingestion of uncooked corn starch (CS) at bedtime. A modified corn starch (MCS) has been shown to prolong euglycemia in some patients. The aim of this study was to evaluate whether stable nighttime glucose control can be achieved with other types of slowly digested carbohydrates in adult GSDI patients.MethodsIn this cross‐over study, nocturnal glucose control and fasting times were assessed with three different nocturnal nutrition regimens in five patients, using continuous glucose monitoring (CGMS) in an outpatient everyday life setting. For each patient, continuous glucose profiles were measured after ingestion of (1) CS, (2) MCS or (3) a pasta meal at bedtime, during 5 to 6 consecutive nights for each regimen.ResultsStable nocturnal glucose control was achieved for all patients with a pasta meal, with a mean duration of glycemia >3.5 mmol/l of 7.6 h (range 5.7‐10.8), and >4 mmol/l of 7 h (5.2‐9.2), similar to CS and MCS. Fasting glucose before breakfast on workdays (after 7.1 ± 0.8 h) was not significantly different between the three interventions (CS 4.1 ± 0.5 mmol/l, MCS 4.6 ± 0.7 mmol/l, pasta 4.3 ± 0.9 mmol/l). During prolonged fasting on weekends, longer duration of normoglycemia was achieved with CS or MCS than with pasta.ConclusionConsumption of cooked pasta is a suitable and more palatable alternative to uncooked corn starch to achieve nighttime glucose control in adult patients with GSDI.
Effects of ankle–foot braces on medial gastrocnemius morphometrics and gait in children with cerebral palsy
Authors: Hösl Matthias , Böhm Harald , Arampatzis Adamantios , Döderlein Leonhard 
Published: June 2015

Purpose In children with cerebral palsy (CP), braces are used to counteract progressive joint and muscle contracture and improve function. We examined the effects of positional ankle–foot braces on contracture of the medial gastrocnemius (MG) and gait in children with CP while referencing to typically developing children. Methods Seventeen independently ambulant children with CP and calf muscle contracture (age 10.4 ± 3.0y) and 17 untreated typically developing peers (age 9.5 ± 2.6y) participated. Children with CP were analysed before and 16 ± 4 weeks after ankle–foot bracing. MG muscle belly length and thickness, tendon and fascicle length, as well as their extensibility were captured by 2D ultrasound and 3D motion capturing during passive, manually applied stretches. In addition, 3D gait analysis was conducted. Results Prior to bracing, the MG muscle–tendon unit in children with CP was 22 % less extensible. At matched amounts of muscle–tendon unit stretch, the muscle belly and fascicles in CP were 7 % and 14 % shorter while the tendon was 11 % longer. Spastic fascicles displayed 32 % less extensibility than controls. Brace wear increased passive dorsiflexion primarily with the knees flexed. During gait, children walked faster and foot lift in swing improved. MG muscle belly and tendon length showed little change, but fascicles further shortened (−11 %) and muscle thickness (−8 %) decreased. Conclusions Use of ankle–foot braces improves function but may lead to a loss of sarcomeres in series, which could explain the shortened fascicles. To potentially induce gastrocnemius muscle growth, braces may also need to extend the knee or complementary training may be necessary to offset the immobilizing effects of braces.
Two Sources of Error in Data on Migration From Mexico to the United States in Mexican Household-Based Surveys
Authors: Hamilton Erin R. , Savinar Robin 
Published: June 2015

AbstractWe examine the nature and degree of two sources of error in data on migration from Mexico to the United States in Mexican household-based surveys: (1) sampling error that results when whole households migrate and no one is left behind to report their migration; and (2) reporting errors that result when migrants are not identified by survey respondents. Using data from the first two waves of the Mexican Family Life Survey, which tracked Mexican migrants to the United States from 2002 to 2005, we find that one-half of migrants from Mexico to the United States are not counted as a result of these two sources of error. Misreporting is the larger source of error, accounting for more than one-third of all migrants. Those who are not counted, especially whole-household migrants, are a unique group. Their omission results in an underestimate of female migrants, child migrants, and migrants from the Mexican border region, and an overestimate of migrants from the periphery region.
Decrease with aging of the microcirculatory function of the lumbar vertebral marrow preceding the loss of bone material density and the onset of intervertebral discal degeneration: A study about the potential cause
Authors: Ou‐yang Lin , Lu Guang‐ming 
Published: June 2015

AbstractObjectiveUsing a dynamic computed tomographic perfusion (CTP) imaging method to explore the age‐related distribution of the microcirculation perfusion function in the vertebral marrow, the bone material density (BMD), and the intervertebral discal degeneration (IDD). Further, to discuss a possible causation relationship between them.MethodsOne hundred and eighty‐six people were randomly enrolled by stratified sampling and grouped by age: ≤15, 16–25, 26–35, 36–45, 46–55, 56–65, 66–75, and ≥76 years old. The average CTP and BMD of the third and fourth lumbar vertebrae marrow were measured and the IDD incidence of the third‐fourth vertebrae was assessed. The temporal–spatial distribution patterns of the age‐related changes of the CTP, BMD, and IDD were described, and the correlations between them were calculated.ResultsThe microcirculatory perfusion function of the vertebral marrow develops to maturity by 25 years and is maintained until age 35, then declines with aging. The BMD grew to a peak from 26 to 45 years old, then decreased yearly. The IDD showed a sudden increase after 45 years of age. The CTP [BF (r = 0.806, P = 0.000), BV (r = 0.685, P = 0.005) and PMB (r = 0.619, P = 0.001)] showed strong positive correlations and CTP [TTP (r = −0.211, P = 0.322) and MTT (r = −0.598, P = 0.002)] showed negative correlations with BMD. The CTP [BF (r = −0.815, P = 0.000), BV (r = −0.753, P = 0.000) and PMB (r = −0.690, P = 0.000)] had strong negative correlations, and CTP [TTP (r = 0.323, P = 0.126) and MTT (r = 0.628, P = 0.001)] had positive correlations with the incidence of IDD.ConclusionThe decrease with aging of the microcirculatory perfusion in the lumbar vertebral marrow preceded, and is a potential causative factor for the loss of BMD and the onset of IDD.
DHA protects against experimental colitis in IL-10-deficient mice associated with the modulation of intestinal epithelial barrier function
Authors: Zhao Jie , Shi Peiliang , Sun Ye , Sun Jing , Dong Jian-Ning , Wang Hong-Gang , Zuo Lu-Gen , Gong Jian-Feng , Li Yi , Gu Li-Li , Li Ning , Li Jie-Shou , Zhu Wei-Ming 
Published: June 2015

A defect in the intestinal barrier is one of the characteristics of Crohn's disease (CD). The tight junction (TJ) changes and death of epithelial cells caused by intestinal inflammation play an important role in the development of CD. DHA, a long-chain PUFA, has been shown to be helpful in treating inflammatory bowel disease in experimental models by inhibiting the NF-κB pathway. The present study aimed at investigating the specific effect of DHA on the intestinal barrier function in IL-10-deficient mice. IL-10-deficient mice (IL-10− / −) at 16 weeks of age with established colitis were treated with DHA (i.g. 35·5 mg/kg per d) for 2 weeks. The severity of their colitis, levels of pro-inflammatory cytokines, epithelial gene expression, the distributions of TJ proteins (occludin and zona occludens (ZO)-1), and epithelial apoptosis in the proximal colon were measured at the end of the experiment. DHA treatment attenuated the established colitis and was associated with reduced infiltration of inflammatory cells in the colonic mucosa, lower mean histological scores and decreased levels of pro-inflammatory cytokines (IL-17, TNF-α and interferon-γ). Moreover, enhanced barrier function was observed in the DHA-treated mice that resulted from attenuated colonic permeability, rescued expression and corrected distributions of occludin and ZO-1. The results of the present study indicate that DHA therapy may ameliorate experimental colitis in IL-10− / − mice by improving the intestinal epithelial barrier function.
The Nordic diet and cognition – The DR's EXTRA Study
Authors: Männikkö Reija , Komulainen Pirjo , Schwab Ursula , Heikkilä Harri M. , Savonen Kai , Hassinen Maija , Hänninen Tuomo , Kivipelto Miia , Rauramaa Rainer 
Published: June 2015

The rapid increase in the prevalence of dementia associated with ageing populations has stimulated interest in identifying modifiable lifestyle factors that could prevent cognitive impairment. One such potential preventive lifestyle factor is the Nordic diet that has been shown to reduce the risk of CVD; however, its effect on cognition has not been studied. The aim of the present study was to estimate the cross-sectional and longitudinal associations of the baseline Nordic diet with cognitive function at baseline and after a 4-year follow-up in a population-based random sample (n1140 women and men, age 57–78 years) as secondary analyses of the Finnish Dose-Responses to Exercise Training study. The Nordic diet score was created based on reported dietary components in 4-d food records. Cognition was assessed by the Consortium to Establish a Registry for Alzheimer's Disease (CERAD) neuropsychological battery and the Mini-mental State Examination (MMSE). The baseline Nordic diet score had been positively associated with Verbal Fluency (β 0·08 (95 % CI 0·00, 0·16),P= 0·039) and Word List Learning (β 0·06 (95 % CI 0·01, 0·10),P= 0·022) at 4 years but not with the Consortium to Establish a Registry for Alzheimer's Disease total score (CERAD-TS) or MMSE at 4 years, after adjustment for baseline cognitive scores, demographic factors and health-related factors. After excluding individuals with impaired cognition at baseline, the baseline Nordic diet score had also been positively associated with the CERAD-TS (β 0·10 (95 % CI 0·00, 0·20),P= 0·042) and MMSE (β 0·03 (95 % CI 0·00, 0·06),P= 0·039) at 4 years. These associations disappeared after further adjustment for energy intake. In conclusion, the Nordic diet might have a positive association with cognition in individuals with normal cognition.
Interactions between pupae of the pine processionary moth (Thaumetopoea pityocampa) and parasitoids in aPinusforest
Authors: Bonsignore C.P. , Manti F. , Castiglione E. 
Published: June 2015

AbstractParasitoids are significant enemies of many economically important insects and there is some evidence to suggest that their actions have a role in terminating the outbreaks of forest Lepidoptera populations. In this study, we examined the impact of parasitoids on the pupae of the pine processionary moth, and highlighted the presence of several parasitoid species for this developmental stage. A higher rate of parasitism was found when the pupal density in the soil was reduced, but the rate of parasitism was not influenced by pupal morphological traits or by the presence or absence of a cocoon around a pupa. Of the external factors examined, a delay in the time of descent of larvae from the trees had a positive effect on the level of parasitism. Observational data indicated that dipteran and hymenopteran were the most abundant parasitoids to emerge from moth pupae. Our study highlights the complexity of the parasitoid–host dynamics, and stresses the importance of carefully determining environmental effects on host–parasitoid relations.
Licence to Kill: The Murderous Outrages Act and the rule of law in colonial India, 1867–1925
Authors: CONDOS MARK 
Published: June 2015

AbstractIn 1867, the Government of India passed one of the most brutal-minded and draconian laws ever created in colonial India. Known as the ‘Murderous Outrages Act’, this law gave colonial officials along the North-West Frontier wide powers to transgress India's legal codes in order to summarily execute and dispose of individuals identified as ‘fanatics’. Arguments for the creation and preservation of this law invariably centred around claims about the purportedly ‘exceptional’ character of frontier governance, particularly the idea that this was a region that existed in a perpetual state of war and crisis. Far from being peripheral in its impact, this article explores how this law both drew upon and enabled a wider legal culture that pervaded India in the wake of 1857. It argues that this law was a signal example of British attempts to mask the brute power of executive authority through legalistic terms, and was also evocative of a distinctly ‘warlike’ logic of colonial legality.
Socialising place attachment: place, social memory and embodied affordances
Authors: DEGNEN CATHRINE 
Published: June 2015

ABSTRACTThe significance of place attachment for later life has been convincingly demonstrated. Scholars have offered useful models that help account for the depth of feeling bound up in place attachment in later life, how this attachment is achieved, and its relevance for belonging and identity. To date, however, this focus has largely been on the individual level of experience. This article draws on sociological and anthropological perspectives to consider how place attachment is forged and experienced in dynamic interaction with other entities and other processes: how place attachment is also a collective, relational and embodied process, caught up and experienced via social memory practices and sensorial, bodily knowledge. This resonates with and contributes to the ‘relational turn’ which has attracted burgeoning interest in the larger home disciplines of sociology, human geography and anthropology, and reciprocally helps them extend and build their interaction with critical ageing studies. In making this argument, I draw on two periods of anthropological, ethnographic participant-observation that I conducted in a semi-rural village in the former coalfields in South Yorkshire, England.
Transcatheter device closure of perimembranous ventricular septal defect in children treated with prophylactic oral steroids: acute and mid-term results of a single-centre, prospective, observational study
Authors: Thakkar Bhavesh , Patel Nehal , Bohora Shomu , Bhalodiya Dharmin , Singh Tarandeep , Madan Tarun , Shah Saurin , Poptani Vishal , Shukla Anand 
Published: June 2015

AbstractBackground and ObjectiveAlthough transcatheter closure of perimembranous ventricular septal defect is emerging as an accepted, viable alternative, conduction disturbances still remain a major concern. Although steroid treatment has shown encouraging results with complete recovery, efficacy of prophylactic use of steroids is still speculative. We aim to study the mid-term outcome of perimembranous ventricular septal defect closure in children who received prophylactic oral steroids.Materials and methodsA prospective study was designed and antegrade device closure was attempted in eligible children who met the following inclusion criteria: age 3–18 years and weight >10 kg, defect diameter ⩽12 mm, and symptomatic, haemodynamic changes or history of infective endocarditis. Prophylactic steroid protocol consisted of 2 weeks oral prednisolone (1 mg/kg/day) initiated immediately after the procedure, and in the event of bradyarrhythmia it was escalated to 2 mg/kg. Patients were regularly followed-up at 1, 6, and 12 months and then annually. Patients with post-procedure heart block underwent Holter monitoring after a minimum of 1 year interval.ResultsBetween May, 2007 and August, 2012, successful device closure was accomplished in 290/297 patients. Mean age and weight were 9±3.12 years and 21±8.27 kg, respectively. The defect measured 5±1.38 mm on echocardiography. Mean fluoroscopy time was 12.98±8.64 minutes. Eight patients with major complications included one each with device embolisation, haemolysis, severe aortic regurgitation, and five with bradyarrhythmias, including complete atrioventricular block in three, Mobitz II in one, and bifascicular block in one. Patients with complete atrioventricular block responded to high-dose steroid and temporary pacemaker. Minor complications included post-procedure heart block (n=22) and blood loss (n=2). At 18.23±13.15 months follow-up, 8/27 (five major, 22 minor) with arrhythmia had persistent post-procedure heart block of no clinical consequences.ConclusionIn our patient population, transcatheter device closure of the perimembranous ventricular septal defect with prophylactic oral steroid resulted in excellent closure rate and acceptably low incidence of conduction disturbances at mid-term follow-up.
Deep-water observation of scalloped hammerhead Sphyrna lewini in the western Indian Ocean off Tanzania
Authors: Moore Alec B.M. , Gates Andrew R. 
Published: June 2015

A scalloped hammerheadSphyrna lewiniwas observed opportunistically from a remotely operated vehicle 1 m off the seabed at 1042 m depth, during hydrocarbon exploration activities in the Ruvuma Basin off Tanzania. The observation, which occurred during night hours, is the deepest accurately recorded for this species and the first deep-water record for the Indian Ocean. The record adds support for the occurrence in deep water during night hours being a widespread and possibly common behaviour in this species, and further expands a small but growing literature that meso- and bathypelagic environments may be of greater importance to elasmobranchs previously considered to be primarily epipelagic.