bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective mono-radioiodination and characterization of a Cell-Penetrating Peptide: L-Tyr-Maurocalcine
Authors: Ahmadi Mitra , Bacot Sandrine , Poillot Cathy , Desruet Marie-Dominique , Perret Pascale , Riou Laurent , Cestèle Sandrine , Couvet Morgane , Bourgoin Sandrine , Sève Michel , De Waard Michel , Ghezzi Catherine 
Published: June 2014

Abstract
Mono-and poly- iodinated peptides form frequently during radioiodination procedures. However, the formation of a single species
in its mono-iodinated form is essential for quantitative studies such as determination of tissue concentration or image
quantification. Therefore, the aim of the present study was to define the optimal experimental conditions in order to
exclusively obtain the mono-iodinated form of L-maurocalcine (L-MCa). L-MCa is an animal venom toxin which was shown to act as
a cell-penetrating peptide. In order to apply the current direct radioiodination technique using oxidative agents including
chloramine T, Iodo-Gen® or lactoperoxidase, an analogue of this peptide containing a tyrosine residue
(Tyr-L-MCa) was synthesized and was shown to fold/oxidize properly. The enzymatic approach using lactoperoxidase/H2O2
was found to be the best method for radioiodination of Tyr-L-MCa. MALDI-TOF mass spectrometry analyses were then used for
identification of the chromatographic eluting components of the reaction mixtures. We observed that the production of different
radioiodinated species depended upon the reaction conditions. Our results successfully described the experimental conditions
of peptide radioiodination allowing the exclusive production of the mono-iodinated form with high radiochemical purity and
without the need for a purification step. Mono-radioiodination of L-Tyr-MCa will be crucial for future quantitative studies,
investigating the mechanism of cell penetration and in vivo biodistribution.
Untersuchung optischer 3D Messsysteme zum Einsatz in der computerassistierten Chirurgie
Authors: Sahm Stephanie , Kerstein Thomas , Scarpin Dominik , Roth Hubert , Wahrburg Jürgen 
Published: June 2014

ZusammenfassungOptische 3D Messsysteme bilden wesentliche Komponenten, um in der computerassistierten Chirurgie die räumliche Lage chirurgischer Instrumente im Bezug auf den Patienten fortlaufend zu erfassen. In diesem Beitrag wird ein auf diese Anwendung zugeschnittener Versuchsaufbau vorgestellt, der die Untersuchung der Genauigkeit optischer, markerbasierter 3D Lokalisiersysteme ermöglicht. Zur berührungslosen Erfassung der Patientenanatomie wird außerdem ein robotergeführter Laserscanner beschrieben, bei dem durch Auswertung der Kamera-Rohdaten des Lokalisiersystems die Laserlinie detektiert wird.
Kompaktes photoakustisches Gasmesssystem mit
Potential zur weiteren Miniaturisierung
Authors: Huber Jochen , Wöllenstein Jürgen 
Published: June 2014

Zusammenfassung
Der photoakustische Effekt kann zur Detektion von IR-aktiven
Gasen genutzt werden. Es wird ein einfaches und kompaktes System
vorgestellt, welches nach theoretischen Vorbetrachtungen
entwickelt wurde. Das System besteht aus einem thermischen
Breitband-IR-Emitter und einer Messkammer mit einem MEMS-Mikrofon
als Detektor. Labormessungen mit den Gasen Kohlendioxid und Methan
zeigen die Eignung als selektives und kostengünstiges
Messsystem. Das Potential zur weiteren Miniaturisierung des
Messsystems wird aufgezeigt.
Development of a Microfluidic GHz Impedance Cytometer
Authors: Haandbæk Niels , Bürgel Sebastian C. , Heer Flavio , Hierlemann Andreas 
Published: June 2014

Abstract
This article presents a novel microfluidic impedance cytometer
enabling dielectric characterization of single cells at
frequencies up to 500 MHz. The dielectric properties of cells at
lower frequencies contain information about their size and
membrane capacitance. The increased frequency range of the
presented cytometer potentially allows for characterization of
intracellular components, such as vacuoles or the cell nuclei. We
demonstrate the overall capabilities of the cytometer through
discrimination of polystyrene beads from Chinese hamster ovary
(CHO) cells. The discrimination is based on the difference in
dielectric properties at frequencies up to 500 MHz.
Verb cognates in Haitian Creole
Authors: Glaude Herby , Zribi-Hertz Anne 
Published: June 2014

AbstractThis article bears on three “verb cognate” (VC) constructions attested in Haitian Creole, where the verb and a homonymous replica of the verb cooccur within the same clausal domain. In the first two constructions – VC1 and VC2 – the verb's cognate is crucially unstressed and supports further lexical material, while in the third construction – VC3, which subsumes the subcase known as
How autosegmental is phonology?
Authors: Hyman Larry M. 
Published: June 2014

AbstractIn this paper I raise the following questions: (i) How appropriate/effective are traditional autosegmental representations? (ii) What insights do autosegmental representations help us express? (iii) Where do autosegmental representations fall short? (iv) What does this all mean for phonology? Although there is still general acceptance of the “basic autosegmental insight”, i.e. that certain features are semi-autonomous from each other and their anchors (e.g. tones vs. tonebearing units), there has been decreasing interest in representational issues in some current approaches to phonology, as well as proposed frameworks which abandon some of the key properties of autosegmental phonology. In this paper I will be particularly interested in the intersection of the following properties assumed in traditional autosegmental phonology: (i) assimilation = spreading; (ii) no line-crossing; (iii) branching elements ≠ successive linking of identical elements. I will first review these three autosegmental assumptions – and the good things they do for us – and then present some unexpected tonal data which are potentially problematic for one or another of these assumptions. After identifying what makes the cited cases problematic and the choices we have in accounting for them, I will conclude with a discussion of the implications such facts have concerning phonology in general.
The Medium and the Message, or, what is »Deuteronomistic« about the Book of Jeremiah?
Authors: Leuchter Mark 
Published: June 2014

Scholars have correctly identified a particular linguistic style as »Deuteronomistic«, but advances in our understanding of scribal practice in antiquity call in question attempts to identify a text as »Deuteronomistic« on the basis of linguistic style alone. A consideration of conceptual agendas and rhetorical choices must accompany any such assignment. Bearing this in mind, the present study returns to the issue of what is »Deuteronomistic« about the book of Jeremiah, identifying conceptual continuity and rhetorical strategies that it shares with the late-monarchic book of Deuteronomy. The present study also considers why the medium of the prophetic book (that of Jeremiah) became such a preferred choice for the development of Deuteronomistic discourse in the post-monarchic period.La recherche a établi légitimement un style »deutéronomiste«, mais des progrès dans la compréhension de la pratique des scribes dans l’Antiquité rendent plus difficile la tentative d’identifier un style comme »deutéronomiste« sur la seule base de ses particularités linguistiques. Il faut également prendre en compte le répertoire conceptuel et les choix rhétoriques. C’est dans cette perspective que l’a. aborde la question: que signifie »deutéronomiste« par rapport au livre de Jérémie? Il identifie la continuité conceptuelle et les stratégies rhétoriques que ce livre a en commun avec le livre du Deutéronome, d’époque royale tardive. Pour finalement s’interroger: pourquoi le médium d’un livre prophétique (Jér.) s’avéra-t-il si approprié pour le développement ultérieur du discours deutéronomiste après la fin de l’époque royale?Die Forschung hat zu Recht einen bestimmten sprachlichen Stil als »deuteronomistisch« identifiziert; aber die Fortschritte in unserem Verständnis der antiken Schreibpraxis erschweren Versuche, einen Text allein aufgrund seines Sprachstils als »deuteronomistisch« zu identifizieren. Eine Berücksichtigung von Begriffskonzeptionen und rhetorischen Wahlmöglichkeiten muss solche Zuweisungen begleiten. Dies im Hinterkopf behaltend, beschäftigt sich der vorliegende Beitrag mit dem Thema, was »deuteronomistisch« in Bezug auf das Jeremiabuch bedeutet, indem er konzeptionelle Kontinuität und rhetorische Strategien identifiziert, die dieses Buch mit dem spät-königszeitlichen Deuteronomium teilt. Schließlich wird überlegt, warum das Medium eines Prophetenbuchs (Jer) für die Weiterentwicklung des deuteronomistischen Diskurses nach dem Ende der Königszeit so besonders geeignet erschien.
Single crystal X-ray and electron microprobe study of Al/Si-disordered anorthite with low content of albite
Authors: Echigo Takuya , Hoshino Mihoko , Kimata Mitsuyoshi , Shimizu Masahiro , Matsui Tomoaki , Nishida Norimasa 
Published: June 2014

Abstract
The crystal chemistry of anorthite with low content of albite (An92.0 Ab3.4), part of a rapidly cooled anorthite megacryst occurring in 1940 ejecta from Miyake-jima volcano, Japan, has been investigated using a single-crystal X-ray diffractometer and an electron microprobe analyzer with wavelength dispersive X-ray spectroscopy (EMPA-WDS). The structure was refined in space group P1̅ and cell parameters, a=8.182(6) Å, b=12.883(4) Å, c=7.092(4) Å, α=93.19(4)°, β=115.91°(4), γ=91.18°(4). The final weighted R-factor is 3.77% for 1549 reflections. Averaged T-O distances are 1.681 Å for T1(0), 1.674 Å for T1(m), 1.677 Å for T2(0) and 1.680 Å for T2(m), indicating Al occupancies of 0.501, 0.453, 0.472, and 0.496, respectively. These results suggest that the Al/Si-distribution in the tetrahedral framework is highly disordered (QOD=0.06), which results in bisection of the c-period in Al/Si ordered anorthites (c∼14 Å). Chemical composition of the refined crystal obtained by EMPA-WDS is (Ca0.93 Na0.03 Fe0.02□0.01)(Mg0.01 Al1.94 Si2.05)O8. The extra-framework site-populations consist of the following two: (1) A(000) site occupied by Ca (86%), Na (8%), Fe2+ (4%) and □(2%), and (2) A(zi0) site by Ca (100%). The Al/Si tetrahedral framework is hence pseudo-face-centered in symmetry (C1ʩ so that both extra-framework cations and their defects appear to reduce the symmetry of the overall structure to P1ʮ Although the Al/Si disordered anorthite can be interpreted as a metastable phase, the observed chemical non-stoichiometry may stabilize such a metastable structure by introducing minor -Si4+-O-Si4+- bonds into the tetrahedral framework and anti-phase-boundary.
A Perspective on the Proposed Amendments to the Drug Distribution Guideline
Authors: Bennardo Kevin 
Published: June 2014

This Commentary provides a perspective on the U.S. Sentencing Commission’s proposed amendment to the drug distribution guideline. The proposed amendment has the potential to substantially affect federal sentencing and incarceration because of the sheer volume of advisory sentencing ranges that are calculated through the drug distribution guideline.
Although the proposed almost-across-the-board offense level reduction is laudable, the Commission should go further in its amendment of the drug distribution guideline. First, the proposed amendment reduces most of the offense levels in the Drug Quantity Table by two levels, but it does not alter the offense levels for distributions of the smallest and largest drug quantities. The amendment would be more internally consistent if the Commission simply reduced the offense levels applicable to the entire Drug Quantity Table by two levels.
Second, the amendment continues to base the offense levels in the Drug Quantity Table on the mandatory minimums in the drug trafficking statute. The Commission should delink the Drug Quantity Table from the mandatory minimums. An increasing number of drug defendants escape the operation of otherwise-applicable mandatory minimum sentences, particularly in the wake of the Supreme Court’s decision in Alleyne v. United States and the Attorney General’s directive to federal prosecutors to structure indictments to avoid mandatory minimums for certain low-level, non-violent drug offenders. Fairness dictates that these offenders receive a advisory sentencing range that reflects the Commission’s research and expertise rather than one that is bound to inapplicable statutory mandatory minimums.
FluorescenceIn SituHybridization and Optical Mapping to Correct Scaffold Arrangement in the Tomato Genome
Authors: Shearer Lindsay A , Anderson Lorinda K , de Jong Hans , Smit Sandra , Goicoechea José Luis , Roe Bruce A , Hua Axin , Giovannoni James J , Stack Stephen M 
Published: June 2014

AbstractThe order and orientation (arrangement) of all 91 sequenced scaffolds in the 12 pseudomolecules of the recently published tomato (Solanum lycopersicum, 2n = 2x = 24) genome sequence were positioned based on marker order in a high-density linkage map. Here, we report the arrangement of these scaffolds determined by two independent physical methods, bacterial artificial chromosome–fluorescence in situ hybridization (BAC-FISH) and optical mapping. By localizing BACs at the ends of scaffolds to spreads of tomato synaptonemal complexes (pachytene chromosomes), we showed that 45 scaffolds, representing one-third of the tomato genome, were arranged differently than predicted by the linkage map. These scaffolds occur mostly in pericentric heterochromatin where 77% of the tomato genome is located and where linkage mapping is less accurate due to reduced crossing over. Although useful for only part of the genome, optical mapping results were in complete agreement with scaffold arrangement by FISH but often disagreed with scaffold arrangement based on the linkage map. The scaffold arrangement based on FISH and optical mapping changes the positions of hundreds of markers in the linkage map, especially in heterochromatin. These results suggest that similar errors exist in pseudomolecules from other large genomes that have been assembled using only linkage maps to predict scaffold arrangement, and these errors can be corrected using FISH and/or optical mapping. Of note, BAC-FISH also permits estimates of the sizes of gaps between scaffolds, and unanchored BACs are often visualized by FISH in gaps between scaffolds and thus represent starting points for filling these gaps.
Genome-wide Screens for Sensitivity to Ionizing Radiation Identify the Fission Yeast Nonhomologous End Joining Factor Xrc4
Authors: Li Jun , Yu Yang , Suo Fang , Sun Ling-Ling , Zhao Dan , Du Li-Lin 
Published: June 2014

AbstractNonhomologous end joining (NHEJ) is the main means for repairing DNA double-strand breaks (DSBs) in human cells. Molecular understanding of NHEJ has benefited from analyses in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe. In human cells, the DNA ligation reaction of the classical NHEJ pathway is carried out by a protein complex composed of DNA ligase IV (LigIV) and XRCC4. In S. cerevisiae, this reaction is catalyzed by a homologous complex composed of Dnl4 and Lif1. Intriguingly, no homolog of XRCC4 has been found in S. pombe, raising the possibility that such a factor may not always be required for classical NHEJ. Here, through screening the ionizing radiation (IR) sensitivity phenotype of a genome-wide fission yeast deletion collection in both the vegetative growth state and the spore state, we identify Xrc4, a highly divergent homolog of human XRCC4. Like other fission yeast NHEJ factors, Xrc4 is critically important for IR resistance of spores, in which no homologous recombination templates are available. Using both extrachromosomal and chromosomal DSB repair assays, we show that Xrc4 is essential for classical NHEJ. Exogenously expressed Xrc4 colocalizes with the LigIV homolog Lig4 at the chromatin region of the nucleus in a mutually dependent manner. Furthermore, like their human counterparts, Xrc4 and Lig4 interact with each other and this interaction requires the inter-BRCT linker and the second BRCT domain of Lig4. Our discovery of Xrc4 suggests that an XRCC4 family protein is universally required for classical NHEJ in eukaryotes.
Clinical Trial Enrollment Among Adolescents With Cancer: Supplement Overview
Authors: Tai Eric , Beaupin Lynda , Bleyer Archie 
Published: June 2014

BACKGROUND:
Survival rates for children with cancer have significantly increased over the past 35 years. However, adolescents with cancer aged 15 to 19 years have had less progress in survival prolongation compared with younger children, which may be due to lower clinical trial enrollment among adolescents with cancer. To help address this issue, the Centers for Disease Control and Prevention (CDC) convened a series of webinars to identify salient issues and measures to address this problem. This supplement is intended to raise awareness about the unique challenges of clinical trial enrollment among adolescents with cancer.

METHODS:
The CDC convened a workgroup of researchers and health care providers in the field of adolescent and young adult oncology and cancer survivorship to examine the barriers and challenges limiting the participation of adolescents in clinical trials and to define ways to improve on these concerns.

RESULTS:
The workgroup identified 3 distinct issues affecting clinical trial enrollment among adolescents with cancer: (1) many adolescents with cancer are not referred to institutions where clinical trials are offered, (2) there are limited numbers of clinical trials for adolescents with cancer, and (3) psychosocial barriers impede adolescents with cancer from enrolling in clinical trials.

CONCLUSIONS:
Adolescents with cancer have the smallest proportion and least number of patients enrolled in clinical trials in pediatric oncology. Successfully addressing this challenge requires improving referral to existing clinical trials, addressing regulatory barriers to clinical trial enrollment, increasing the number of clinical trials for adolescents, and addressing unique psychosocial barriers to clinical trial enrollment.
Treatment Setting, Clinical Trial Enrollment, and Subsequent Outcomes Among Adolescents With Cancer: A Literature Review
Authors: Tai Eric , Buchanan Natasha , Westervelt Lauren , Elimam Dena , Lawvere Silvana 
Published: June 2014

BACKGROUND:
There has been an overall improvement in survival rates for persons with cancer over the past 35 years. However, these gains are less prevalent among adolescents with cancer aged 15 to 19 years, which may be due to lower clinical trial enrollment among adolescents with cancer.

METHODS:
We conducted a literature review to assess current research regarding clinical trial enrollment and subsequent outcomes among adolescents with cancer. The search included English-language publications that reported original data from January 1985 to October 2011.

RESULTS:
The search identified 539 records. Of these 539 records, there were 30 relevant original research articles. Multiple studies reported that adolescents with cancer are enrolled in clinical trials at lower rates compared with younger children and older adults. Treatment setting, physician type, and institution type may all be factors in the low enrollment rate among adolescents. Few data focused solely on adolescents, with many studies combining adolescents with young adults. The number of available studies related to this topic was limited, with significant variability in study design, methods, and outcomes.

CONCLUSIONS:
This literature review suggests that adolescents with cancer are not treated at optimal settings and are enrolled in clinical trials at low rates. This may lead to inferior treatment and poor subsequent medical and psychosocial outcomes. The scarcity in data further validates the need for additional research focusing on this population.
Morphology-driven electrical and optical properties in graded hierarchical transparent conducting Al:ZnO
Authors: Gondoni P. , Mazzolini P. , Pillado Pérez A. M. , Russo V. , Li Bassi A. , Casari C. S. 
Published: June 2014

ABSTRACTGraded Al-doped ZnO layers, constituted by a mesoporous forest-like system evolving into a compact transparent conductor, were synthesized by Pulsed Laser Deposition with different morphologies to study the correlation with functional properties. Morphology was monitored by Scanning Electron Microscopy images and by measuring the resulting surface roughness. Its effects on electrical conductivity – especially carrier mobility, which significantly decreases with increasing roughness – allow to discuss the limitations in conduction mechanisms. Significant changes in light scattering capability due to variations in morphology are also investigated and discussed to study the correlation between morphology and functional properties.
Fatores associados à persistência à terapia hormonal em mulheres com câncer de mama
Authors: Brito Cláudia , Portela Margareth Crisóstomo , Vasconcellos Mauricio Teixeira Leite de 
Published: June 2014

OBJETIVO Analisar os fatores associados à persistência à hormonioterapia para câncer de mama visando à melhoria da qualidade do cuidado prestado. MÉTODOS Estudo longitudinal a partir de dados secundários. Foi analisada uma coorte de 5.861 mulheres com câncer de mama registradas em diferentes bancos de dados do Instituto Nacional de Câncer e do Sistema Único de Saúde. Todas as pacientes foram tratadas nesse hospital, que dispensa a medicação gratuitamente, e o período de seguimento foi de janeiro de 2004 a outubro de 2010. Variáveis sociodemográficas, comportamentais, clínicas, de estilos de vida e de aspectos do serviço de saúde integraram-se à análise para testar associação com a persistência ao tratamento hormonal, pelo método de Kaplan-Meier e Riscos Proporcionais de Cox. RESULTADOS A persistência geral à hormonioterapia foi de 79,0% ao final do primeiro ano e 31,0% em cinco anos de tratamento. O risco de descontinuidade à hormonioterapia mostrou-se maior entre mulheres com idade inferior a 35 anos, com estadiamento mais grave (III e IV), usuárias de álcool, que realizaram quimioterapia, e para cada hospitalização, cada exame e cada mês, entre o diagnóstico e o início do tratamento adicional. Na direção oposta, o risco de descontinuidade mostrou-se menor entre as mulheres com nível médio e superior de escolaridade, com companheiro, com história familiar de câncer, submetidas à cirurgia e que tiveram consultas com mastologista e com oncologista clínico. CONCLUSÕES Das mulheres com câncer de mama, 69,0% não persistiram ao término de cinco anos do tratamento hormonal, aumentando o risco de uma resposta clínica inadequada. Os resultados mostram aspectos do cuidado que podem conduzir a melhores respostas ao tratamento.