bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microwave-Assisted Synthesis of Heterocyclic Scaffolds
Authors: Agarwal Alka , Kumar Sanjeev , Maurya Anand 
Published: July 2024

AbstractIn recent years, there has been a notable surge in the utilization of microwave energy, leading to the emergence of innovative and groundbreaking methods across various branches of chemistry, including organic synthesis, materials science, heterocyclic chemistry, and medicinal chemistry. This comprehensive literature review delves into the microwave-assisted organic synthesis of specific heterocycles, illuminating its effectiveness in producing diverse molecules with heightened efficiency and selectivity. The review highlights the significant role of microwave irradiation as a potent method for constructing a wide range of compounds. Particular emphasis is placed on the impact of the technique on synthesizing various hybrids such as 1,2,3-triazole hybrids, coumarin hybrids, imidazopyridine hybrids, phenanthridines hybrids, carbene hybrids, and oxazole hybrids. This article is valuable as it offers insights into current synthetic procedures and trends in developing innovative medications utilizing heterocyclic compounds.1	Introduction2	Synthesis of 1,2,3-Triazole Hybrids3	Coumarin Hybrids4	Imidazo Pyridine Hybrids5	Phenanthridine Hybrids6	Carbene Hybrids7	Oxazole Hybrids8	Conclusion
Self-reported hearing loss in urban Aboriginal and Torres Strait Islander adults: unmeasured, unknown and unmanaged
Authors: Pender Alice M. , Schluter Philip J. , Bainbridge Roxanne G. , Spurling Geoffrey K. , Wilson Wayne J. , Tyson Claudette ‘Sissy’ , Askew Deborah A. 
Published: June 2024

Background Effective management of hearing loss in adults is fundamental for communication, relationships, employment, and learning. This study examined the rates and management of self-reported hearing loss in urban Aboriginal and Torres Strait Islander adults. Methods A retrospective, observational study of Aboriginal and Torres Strait Islander people aged ≥15 years who had annual health checks at an urban Aboriginal and Torres Strait Islander primary healthcare clinic in Inala, Queensland, was conducted to determine self-reported hearing loss rates by age and ethnic groups stratified by sex. A medical record audit of patients who self-reported hearing loss from January to June 2021 was performed to identify current management approaches, and the proportion of patients that were appropriately managed. Results Of the 1735 patients (average age 40.7 years, range 15.0–88.5 years, 900 [52.0%] women) who completed 3090 health checks between July 2018 and September 2021, 18.8% self-reported hearing loss. Rates did not differ between men and women. However, significant effects were noted for age, with rates increasing from 10.7% for patients aged 15–24 years to 38.7% for those aged ≥65 years. An audit of 73 patient medical records revealed that 39.7% of patients with self-reported hearing loss were referred to Ear, Nose and Throat/audiology or received other management. A total of 17.8% of patients owned hearing aids. Conclusions Only 40% of Aboriginal and Torres Strait Islander adults who self-reported hearing loss were referred for management. Significant changes to clinical management and government-funded referral options for hearing services are required to improve the management of self-reported hearing loss in this population.
Evaluation of ethanol-gasoline blends in SI engines using experimental and ANN techniques
Authors: Hofny Mohamed S , Ghazaly Nouby M , N Shmroukh Ahmed , Abouelsoud Mostafa 
Published: July 2024

Abstract
Fuel combustion has become a major global concern, with much research focusing on the various emissions resulting from different types of fuels. Due to the harmful pollutant emissions from fossil fuels, the world has turned to renewable and alternative fuels to limit toxic emissions and greenhouse effects. Ethanol is a biofuel that, when used in spark ignition engines with gasoline can improve the octane number, combustion efficiency, and produce less emissions. The current research studies the effect of different ethanol blends E0, E5, E10, and E15 with gasoline 92 on engine performance parameters and emissions of a GX35 four-stroke engine at different engine speeds. The results along the speed range reveal that increasing ethanol amount leads to an average increase of 2.7%, 1%, and 1.1% in brake power (BP), brake thermal efficiency (BTE), and CO2 emissions, respectively. Meanwhile, it causes an average decrease of 28 °C, 3%, 15 ppm, and 0.18% in exhaust gas temperature (EGT), brake-specific fuel consumption (BSFC), HC, and CO emissions respectively. Moreover, the current study develops an Artificial Neural Networks (ANN) model for predicting the performance and emissions of spark ignition (SI) engines. Python programming language is used for ANN coding to train and validate the ANN model with E15. Regression plots were generated to visualize the correlation between the target and predicted data, indicating outstanding performance. The results confirmed the model’s reliability for BP, EGT, CO, CO2, and HC parameters with R2 values more than 0.99 and with acceptable performance for BSFC and BTE with R2 of 0.9339, and 0.9708, respectively. To ensure that the is no overfitting during the ANN study, we used different statistical methods, such as validation set, cross-validation, and learning curves.
Getting precise about gender and sex measurement: a primer for epidemiologists
Authors: Restar Arjee J , Lett Elle , Menezes Neia Prata , Molino Andrea R , Poteat Tonia C , Dean Lorraine T , Glick Jennifer L , Baker Kellan E , Cole S Wilson 
Published: June 2024

Abstract
Accurately measuring gender and sex is crucial in public health and epidemiology. Iteratively reexamining how variables—including gender and sex—are conceptualized and operationalized is necessary to achieve impactful research. Reexamining gender and sex advances epidemiology toward its goals of health promotion and disease elimination. While we cannot reduce the complexities of sex and gender to simply an issue of measurement, striving to capture these concepts and experiences accurately must be an ongoing dialogue and practice—to the benefit of the field and population health. We assert that epidemiology must counteract misconceptions and accurately measure gender and sex in epidemiology. We aim to summarize existing critiques and guiding principles in measuring gender and sex that can be applied in practice.
Prenatal exposure to benzodiazepine and z-hypnotics and fifth-grade scholastic skills—emulating target trials using data from the Norwegian Mother, Father and Child Cohort Study
Authors: Sundbakk Lene Maria , Wood Mollie , Gran Jon Michael , Nordeng Hedvig 
Published: June 2024

Abstract
Evidence is limited regarding the effect of prenatal benzodiazepine and z-hypnotic exposure and long-term neurodevelopment in childhood. The objective of this study was to investigate the effects of initiating benzodiazepine or z-hypnotic treatment in early, mid, and late pregnancy on fifth-grade numeracy and literacy scholastic skills in children by emulating 3 target trials. The trials are identical except for the timing of enrollment and the number of eligible individuals. Eligibility to the trials required a history of anxiety and/or depression prior to pregnancy. We used data from the Norwegian Mother, Father and Child Cohort Study, linked to the Medical Birth Registry of Norway, to emulate the trials. We adjusted for baseline covariates that were available at time 0 for each trial by inverse probability of treatment weighting using the propensity score. The findings of this study did not show any effect of mothers’ initiation of treatment with benzodiazepines or z-hypnotics in early, mid, or late pregnancy on the children’s fifth-grade test scores in numeracy and literacy. The study results provide reassurance for patients in need of benzodiazepines and z-hypnotics during pregnancy; however, these findings need to be interpreted with caution due to low study power in some of the analyses.
This article is part of a Special Collection on Pharmacoepidemiology.
A large study of metabolomics reveals common and distinct metabolic biomarkers for type 2 diabetes, coronary heart disease, and stroke
Authors: Lu Yanqiang , Li Guochen , Viallon Vivian , Ferrari Pietro , Freisling Heinz , Qiao Yanan , Shao Liping , Wu Luying , Ding Yi , Ke Chaofu 
Published: June 2024

Abstract
We aimed at examining the shared and unique associations of metabolites with multiple cardiometabolic diseases, including type 2 diabetes (T2D), coronary heart disease (CHD), and stroke. In this study, a total of 168 plasma metabolites were measured by high-throughput nuclear magnetic resonance spectroscopy among 98 162 participants free of T2D, CHD, and stroke at baseline. Cox proportional hazard models estimated hazard ratios for a 1-SD increase in metabolite concentration levels, and false discovery rate (at 10%) was used to correct for multiple comparisons. Over 12.1 years of follow-up on average, 3463 T2D, 6186 CHD, and 1892 stroke events were recorded. Most lipoprotein metabolites were associated with risks of T2D and CHD but not with the risk of stroke, with stronger associations for T2D than for CHD. Phospholipids within intermediate-density lipoprotein or large low-density lipoprotein particles showed positive associations with CHD and inverse associations with T2D. Metabolites indicating very small very low-density lipoprotein, histidine, creatinine, albumin, and glycoprotein acetyls were associated with risks of all 3 conditions. This large-scale metabolomics study revealed common and distinct metabolic biomarkers for T2D, CHD, and stroke, providing instrumental information to possibly implement precision medicine for preventing and treating these conditions.
Visual cues do not function in a multimodal signalling context for mate attraction in eastern gray treefrogs
Authors: Augusto-Alves Guilherme , Feagles Olivia S , Toledo Luís Felipe , Höbel Gerlinde 
Published: June 2024

Abstract
Anurans use different sensory modalities to communicate and interact socially, including acoustic, chemical, tactile, and visual signals. In a multimodal context, these sensory modes can transmit different information to the receiver or even reinforce the same message. In this study, we hypothesized that body colour traits and vocal sac movements of male eastern gray treefrogs [Dryophytes versicolor (= Hyla versicolor)] serve as secondary visual cues that increase their attractiveness while calling to attract mates. We tested this hypothesis using playback trials combining synthetic advertisement calls with a variety of visual cues, including differently coloured resin frog models, simulated vocal sacs, and live frogs. None of the visual stimuli tested impacted female choice, and there was no difference in response rate, response time, choice angle, or distance covered in the arena between multimodal and unimodal stimuli. However, females showed a strong preference for longer calls even if the less attractive calls were paired with a visual stimulus. The study provides comprehensive insights into the role of visual stimuli in the mate attraction of eastern gray treefrogs and suggests that call traits play a dominant role in the male-selection process of this species, contrary to what was observed for visual stimuli.
Ocular Syphilis in Patients With Nonreactive Rapid Plasma Reagin and Positive Treponemal Serologies: A Retrospective Observational Cohort Study
Authors: Mohareb Amir M , Barshak Miriam B , Papaliodis George N , Sobrin Lucia , Durand Marlene L 
Published: June 2024

Abstract

Background
Screening for syphilis increasingly relies on positive treponemal rather than nontreponemal tests (rapid plasma reagin [RPR]). We compared ocular syphilis in patients with nonreactive versus positive RPR.

Methods
We conducted a retrospective observational cohort study of ocular syphilis treated at 2 New England hospitals during 1996–2021 based on ophthalmologist-diagnosed eye findings and positive treponemal serology, regardless of RPR. We excluded patients with alternative diagnoses. We categorized RPR into nonreactive RPR, low-titer RPR (&lt;1:8), and high-titer RPR (≥1:8) and compared early and long-term response to therapy.

Results
Our sample included 115 patients with ocular syphilis (median follow-up, 2.5 years): 25 (22%) with nonreactive RPR, 21 (18%) low-titer RPR, and 69 (60%) high-titer RPR. Compared with nonreactive and low-titer RPR, people with high-titer RPR were younger (mean 47 years, P &lt; .001), more likely to be male (93%, P &lt; .001) and more likely to be living with human immunodeficiency virus (49%, P &lt; .001). People with nonreactive and low-titer RPR were less likely than those with high-titer RPR to have posterior uveitis/panuveitis (32% and 29% vs 75%, P &lt; .001) or abnormal cerebrospinal fluid (26% and 35% vs 75%, P &lt; .001), and more likely to present with chronic eye findings (20% and 29% vs 1%, P &lt; .001). In long-term follow-up, eye findings improved and did not recur in most patients (62% nonreactive, 68% low-titer, 96% high-titer RPR); improved but recurred in 29%, 11%, and 4%, respectively; and were stable in 10%, 21%, and 0%, respectively.

Conclusions
Patients with ocular syphilis and nonreactive RPR are similar to patients with low-titer RPR, and antibiotic therapy is beneficial in most.
It is not only about having good attitudes: factor exploration of the attitudes toward security recommendations
Authors: Toro-Jarrin Miguel A , Pazos Pilar , Padilla Miguel A 
Published: July 2024

Abstract
Numerous factors determine information security-related actions (IS-actions) in the workplace. Attitudes toward following security rules and recommendations and attitudes toward specific IS actions determine intentions associated with those actions. IS research has examined the role of the instrumental aspect of attitudes. However, authors argue that attitudes toward a behavioral object are a multidimensional construct. We examined the dimensionality of attitudes toward security recommendations, hypothesized its multidimensional nature, and developed a new scale [attitudes toward security recommendations (ASR scale)]. The results indicated the multidimensional nature of attitudes toward security recommendations supporting our hypothesis. The results revealed two dimensions corresponding to the perceived legitimacy and effectiveness of security recommendations and its perceived rigor. The new ASR scale showed good psychometric properties. This work contributes to the IS research at suggesting that attitudes are a multidimensional construct in the IS context. These findings imply that the employee’s evaluation of information security policy can be examined considering their instrumentality (security recommendations are important) and rigor (security recommendations are strict). Different effects of the dimensions of attitudes over IS-action suggest different interventions. Additionally, this study offers the ASR scale as a new instrument to capture employees’ evaluation of security recommendations.
Cardiac resynchronization therapy–defibrillator implantation with shock lead placement in the left bundle branch area: a case report
Authors: Yoshida Kenta , Yoshino Mitsuru , Kawabata Tokuma , Tasaka Hiroshi , Kadota Kazushige 
Published: July 2024

Abstract

Background
Cardiac resynchronization therapy (CRT) with biventricular pacing is a well-established therapy. Left bundle branch area pacing (LBBAP) is a safe technique providing physiological pacing, and LBBAP-optimized CRT (LOT-CRT) has been shown to provide better electrical resynchronization than traditional CRT. However, there are few reports on shock lead placement in the left bundle branch area (LBBA) during CRT–defibrillator (CRT-D) implantation.

Case summary
A 76-year-old woman with heart failure from dilated cardiomyopathy presented with left bundle branch block pattern (QRS duration, 160 ms). Left ventricular ejection fraction was 21%. Cardiac resynchronization therapy–defibrillator implantation was performed due to worsening symptoms. By reshaping the Agilis HisPro catheter and adding a septal curve, the shock lead was placed deep into the ventricular septum, narrowing QRS duration to 114 ms. Left ventricular activation time was 84 ms. A defibrillation threshold test confirmed successful treatment without adverse events. At 6-month follow-up, left ventricular ejection fraction improved from 21 to 63%, with the patient's condition improving from New York Heart Association class III to class I.

Discussion
It was reported that QRS narrowing in CRT was related to long-term mortality, and LOT-CRT further decreased QRS duration as compared with LBBP only or biventricular pacing and increased the response rate. Combining LBBAP with coronary sinus pacing can potentially achieve superior electrical resynchronization. Lack of a suitable tool for direct shock lead placement in LBBA necessitated additional LBBAP lead in conventional LOT-CRT. Our successful LOT-CRT-D procedure with minimal number of leads through Agilis HisPro catheter reshaping enabled direct LBBA shock lead placement.
Antibiotic resistance alters the ability of Pseudomonas aeruginosa to invade bacteria from the respiratory microbiome
Authors: Lindon Selina , Shah Sarah , Gifford Danna R , Lood Cédric , Gomis Font Maria A , Kaur Divjot , Oliver Antonio , MacLean R Craig , Wheatley Rachel M 
Published: June 2024

Abstract
The emergence and spread of antibiotic resistance in bacterial pathogens is a global health threat. One important unanswered question is how antibiotic resistance influences the ability of a pathogen to invade the host-associated microbiome. Here we investigate how antibiotic resistance impacts the ability of a bacterial pathogen to invade bacteria from the microbiome, using the opportunistic bacterial pathogen Pseudomonas aeruginosa and the respiratory microbiome as our model system. We measure the ability of P. aeruginosa spontaneous antibiotic-resistant mutants to invade pre-established cultures of commensal respiratory microbes in an assay that allows us to link specific resistance mutations with changes in invasion ability. While commensal respiratory microbes tend to provide some degree of resistance to P. aeruginosa invasion, antibiotic resistance is a double-edged sword that can either help or hinder the ability of P. aeruginosa to invade. The directionality of this help or hindrance depends on both P. aeruginosa genotype and respiratory microbe identity. Specific resistance mutations in genes involved in multidrug efflux pump regulation are shown to facilitate the invasion of P. aeruginosa into Staphylococcus lugdunensis, yet impair invasion into Rothia mucilaginosa and Staphylococcus epidermidis. Streptococcus species provide the strongest resistance to P. aeruginosa invasion, and this is maintained regardless of antibiotic resistance genotype. Our study demonstrates how the cost of mutations that provide enhanced antibiotic resistance in P. aeruginosa can crucially depend on community context. We suggest that attempts to manipulate the microbiome should focus on promoting the growth of commensals that can increase the fitness costs associated with antibiotic resistance and provide robust inhibition of both wildtype and antibiotic-resistant pathogen strains.
O-002 CRISPR-Cas, from a bacterial defense system to a novel genetic tool in humans
Authors: Staals R 
Published: July 2024

Abstract
In a span of merely a decade, the CRISPR-Cas technology has revolutionized the scientific, industrial, and medical sectors. Its ability to serve as a highly precise genome editing tool has gained a lot of attention over these years, leading to its recognition with the Nobel Prize in Chemistry in 2020. Amidst these achievements, it is easy to overlook the natural role of the CRISPR-Cas system, which functions as an adaptive immune system against viruses in bacteria. The ongoing exploration and characterization of various variants of these systems continue to pave the way for the development of innovative genetic tools and the enhancement of existing ones. In this presentation, I will start with an explanation of the natural role of CRISPR-Cas in bacteria. This will be followed by a brief overview of how these systems were repurposed into the aforementioned genome editing tools. Subsequently, I will delve into some of the latest advancements (and their bottlenecks) in the field, including a few unconventional applications, and discuss their potential short-term and long-term impacts on human reproduction.
O-039 Con - the faster the better
Authors: Kovacic B 
Published: July 2024

Abstract
Late o’clock: Are slow growing embryos clinically usable?
Con - the faster the better
Reproductive medicine and, within it, embryology, have experienced milestones in the understanding of pre-implantation embryonic development over the last quarter of a century. The development of blastocyst culture media for embryo culture even after embryonic genome activation and the possibility of continuous monitoring of embryonic morphokinetics throughout the pre-implantation period have been major contributors. The development of molecular genetic techniques has also played an important role. The introduction of new cryopreservation techniques has also improved the success rate of the clinical use of surplus embryos, including slow-growing embryos not otherwise selected for fresh cycle transfer. Two questions are most frequently encountered in clinical embryology today: which embryo in a cohort has the best chance of resulting in a live-born child, and how slow and morphologically suboptimal can an embryo be to still be considered clinically useful?
In particular, the latter question does not have an easy answer, as embryos demonstrate varying pace of development. A further problem arises if there are no optimal embryos available in the cohort and this answer has to be given on day 2, 3 or 5, as this is when the embryo transfer is scheduled to take place. Some studies suggest that longer timing of any stage of embryonic development from pronuclear fading onwards means that the embryo is late in development due to certain abnormalities and is likely to be energetically depleted by the activation of its repair mechanisms. Such embryos not only have a lower implantation potential compared to fast embryos, but also diverge in time from the optimal receptivity of the endometrium. Additional testing and/or storage of slow-growing embryos brings the question of their clinical usability to the time of frozen embryo replacement cycles and quiets the couple's expectation of a “successful” completion of the stimulated cycle. Whether embryos are to be selected for fresh ET or FER, the “the faster - the better” principle is likely to be applied. In daily clinical practice, decisions on the use of slow-growing and/or morphologically sub-optimal embryos and the timing of embryo transfer are made on an individual basis, taking into account various medical as well as non-medical (e.g. economic, logistical) factors. These may also include portraying a positive image of the centre's performance. However, patients expect infertility specialists to make realistic assessments not only of the viability of slow growing embryos, but also of the outcome of the transfer of them. The assessment must be based not only on medical and ethical but also on rational grounds.