bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell Contact–Dependent Priming and Fc Interaction with CD32+ Immune Cells Contribute to the TGN1412-Triggered Cytokine Response
Authors: Bartholomaeus Patrick , Semmler Linda Y. , Bukur Thomas , Boisguerin Valesca , Römer Paula S. , Tabares Paula , Chuvpilo Sergey , Tyrsin Dmitry Y. , Matskevich Alexey , Hengel Hartmut , Castle John , Hünig Thomas , Kalinke Ulrich 
Published: January 2014

Abstract
Following inconspicuous preclinical testing, the superagonistic anti-CD28 mAb TGN1412 was applied to six study participants who all developed a devastating cytokine storm. We verified that TGN1412 treatment of fresh PBMCs induced only moderate responses, whereas restoration of tissue-like conditions by high-density preculture (HDC) allowed vigorous cytokine production. TGN1412 treatment of T cells isolated from HDC-PBMCs induced moderate cytokine responses, which upon additional anti-IgG crosslinking were significantly boosted. Moreover, coincubation of TGN1412-treated T cells with B cells expressing the intermediate affinity Fcγ receptor IIB (CD32B), or coincubation with CD32B+ transfectants, resulted in robust T cell activation. This was surprising because TGN1412 was expressed as an Ig of the subclass 4 (IgG4), which was shown before to exhibit only minor affinity to FcγRs. Transcriptome analysis of TGN1412-treated T cells revealed that similar gene signatures were induced irrespective of whether T cells derived from fresh or HDC-PBMCs were studied. Collectively, these data indicate that HDC-PBMCs and HDC-PBMC–derived T cells mount rapid TGN1412 responses, which are massively boosted by FcγR crosslinking, in particular by CD32-expressing B cells. These results qualify HDC-PBMCs as a valuable in vitro test system for the analysis of complex mAb functions.
Tabbed Tissue Expanders Improve Breast Symmetry Scores in Breast Reconstruction
Authors: Khavanin Nima , Gust Madeleine J , Grant David W , Nguyen Khang T , Kim John YS 
Published: January 2014

Background Achieving symmetry is a key goal in breast reconstruction. Anatomically shaped tabbed expanders are a new tool in the armamentarium of the breast reconstruction surgeon. Suture tabs allow for full control over the expander position and thus inframammary fold position, and, in theory, tabbed expanders mitigate many factors responsible for poor symmetry. The impact of a tabbed expander on breast symmetry, however, has not been formally reported. This study aims to evaluate breast symmetry following expander-implant reconstruction using tabbed and non-tabbed tissue expanders.
Methods A chart review was performed of 188 consecutive expander-implant reconstructions that met the inclusion criteria of adequate follow-up data and postoperative photographs. Demographic, oncologic, postoperative complication, and photographic data was obtained for each patient. The photographic data was scored using a 4-point scale assessing breast symmetry by three blinded, independent reviewers.
Results Of the 188 patients, 74 underwent reconstruction with tabbed expanders and 114 with non-tabbed expanders. The tabbed cohort had significantly higher symmetry scores than the non-tabbed cohort (2.82/4±0.86 vs. 2.55/4±0.92, P=0.034).
Conclusions The use of tabbed tissue expanders improves breast symmetry in tissue expander-implant-based breast reconstruction. Fixation of the expander to the chest wall allows for more precise control over its location and counteracts the day-to-day translational forces that may influence the shape and location of the expander pocket, mitigating many factors responsible for breast asymmetry.
Ozone photolysis: Strong isotopologue/isotopomer selectivity in the stratosphere
Authors: Ndengué Steve , Madronich Sasha , Gatti Fabien , Meyer Hans‐Dieter , Motapon Ousmanou , Jost Rémy 
Published: January 2014

AbstractWe calculated the photolysis rate coefficients of five ozone isotopologues/isotopomers as functions of altitude up to 80 km using recent ab initio absorption cross sections and an averaged actinic flux. Three of the five ozone isotopologues/isotopomers are symmetric, with a single isotopic dissociation channel, and two are asymmetric with two isotopic dissociation channels. The specific contributions of the Chappuis, Huggins, and Hartley bands to the photolysis rates and enrichments have been determined as a function of altitude. The Chappuis and Hartley bands have a dominant contribution to the photolysis rates, respectively at low and high altitudes, but these two bands are characterized by small fractionations. In contrast, the Huggins band has a minor contribution to the overall photolysis rate at any altitude, but it generates most of the strong fractionation which peaks around 35 km. The photolysis fractionations are “mass dependent” in contrast with those due to the ozone formation process which are “non–mass dependent.” The altitude dependences of our photolysis fractionations are in qualitative agreement with those of Liang et al. (2006) but differ quantitatively, most notably because the contribution of the Huggins band has been reevaluated. The branching ratio between the two electronic dissociation channels (either O(3P) or O(1D) products) has been used to calculate the isotopic enrichments of the reactive O(1D) induced by ozone photolysis. The isotopic photolysis rates can be included in a global kinetic model that includes ozone formation processes and collision with O2 and other oxygen‐containing species.
Characterizing the Effect of Cytochrome P450 (CYP) 2C8, CYP2C9, and CYP2D6 Genetic Polymorphisms on Stereoselective N‐demethylation of Fluoxetine
Authors: Wang Zhangting , Wang Shengjia , Huang Minmin , Hu Haihong , Yu Lushan , Zeng Su 
Published: January 2014

ABSTRACTFluoxetine (FLX) is one of the most widely prescribed selective serotonin reuptake inhibitors. Although FLX is used as racemate in the clinic, the clinical pharmacokinetics of FLX and its N‐demethylation metabolite norfluoxetine (NFLX) show obvious cytochrome P450 (CYP) polymorphism dependency and exhibit marked stereoselectivity. However, the kinetic profiles of CYP variants to FLX remain unclear. In the present study, some variants of human CYP2C8, CYP2C9, and CYP2D6 were first expressed in insect cells, and their catalytic roles with respect to FLX enantiomers were then investigated. CYP2C8.4 and CYP2C9.10 showed significantly lower activity and CYP2C8.3 showed significantly higher activity toward both R‐ and S‐FLX compared with the wildtype, while CYP2C9.3, CYP2C9.13, and CYP2C9.16 showed significantly lower activity only toward R‐FLX. Five CYP2C9 variants and CYP2D6.1 exhibited significantly stereoselective kinetic profiles prior to R‐FLX, and CYP2C8.3 showed a slight stereoselectivity. Interestingly, obvious substrate inhibition was observed in the CYP2C9 wildtype and its three variants only in the case of R‐FLX. Together, these findings suggest that CYP2C9 and CYP2D6 polymorphism may play an important role in the clearance of FLX and also in the stereoselective kinetic profiles of FLX enantiomers. Chirality 26:166–173, 2014. © 2014 Wiley Periodicals, Inc.
A flume experiment on wood storage and remobilization in braided river systems
Authors: Bertoldi Walter , Welber Matilde , Mao Luca , Zanella Sandra , Comiti Francesco 
Published: January 2014

ABSTRACTThis work investigates wood dynamics in braided streams through physical modelling in a mobile bed laboratory flume, with the specific objective to characterize wood storage and turnover as a function of wood input rate and of wood element type. Three parallel channels (1.7 m wide, 10 m long) filled with uniform sand were used to reproduce braided networks with constant water discharge and sediment feeding. Wood dowels with and without simplified root wads were regularly added at the upstream end of each flume at different input rates, with a 1:2:3 ratio between the three flumes. Temporal evolution of wood deposition patterns and remobilization rates were monitored by a series of vertical images that permitted the recognition of individual logs. Results show that wood tends to disperse in generally small accumulations (< 5 logs), with higher spatial density on top of sediment bars, and is frequently remobilized due to the intense morphological changes. The amount of wood stored in the channel depends on log input rate through a non‐linear relationship, and input rates exceeding approximately 100 logs/hour determine a sharp change in wood dynamics, with higher storage volume and augmented formation of large jams (> 10 elements) that are less prone to remobilization. Presence of root wads seems to play a minor role in wood deposition, but it reduces the average travel distance of logs. Turnover rates of logs were similar in the three flumes, independently of wood input rate and largely resembling the turnover rate of exposed bars. For the simulated conditions, significant effects of wood on bed morphology were not observed, suggesting that interactions with fine sediments and living vegetation are crucial to form large, stable wood jams able to bring about relevant morphological changes. Copyright © 2014 John Wiley & Sons, Ltd.
Twenty‐First Century Employment Relationships: The Case for an Altruistic Model
Authors: Clarkson Gail P. 
Published: January 2014

AbstractDifferent parties in any form of employment relationship (ER) will display variation in terms of their motivations and what they want to accomplish. Insights into how we might meet these potentially competing interests come from the discipline of sociobiology. In particular, this article focuses upon multilevel selection (MLS); the central tenet being that internally altruistic groups are likely to outperform their more selfish counterparts, which many contemporary theoretical biologists agree is a legitimate theory accounting for evolutionary change and success. On the assumption that the interests of all parties are more likely to be realized in the context of overall organizational success, the simple logic of MLS challenges the quid pro quo arrangements central to dominant theoretical perspectives of the ER. Drawing upon the growing evidence in support of MLS theory, and long‐accepted insights from social psychology, the case for altruism as a model of the ER fit for the twenty‐first century world of work is presented and consideration is given as to how altruistic behaviors can be encouraged in all parties. © 2014 Wiley Periodicals, Inc.
LIF/STAT3/SOCS3 Signaling Pathway in Murine Bone Marrow Stromal Cells Suppresses Osteoblast Differentiation
Authors: Matsushita Kenta , Itoh Shousaku , Ikeda Shun , Yamamoto Yumiko , Yamauchi Yukako , Hayashi Mikako 
Published: January 2014

ABSTRACTLeukemia inhibitory factor (LIF) is a pleiotropic cytokine that belongs to the interleukin‐6 family and is expressed by multiple tissue types. This study analyzed the effect of LIF on osteoblast differentiation using primary murine bone marrow stromal cells (BMSCs). Colony‐forming unit‐osteoblast formation by BMSCs was significantly suppressed by LIF treatment. To clarify the mechanism underlying the LIF suppressive effect on osteoblast differentiation, we analyzed the downstream signaling pathway of LIF. LIF/signal transducer and activator of transcription 3 (STAT3) signaling induces the expression of suppressor of cytokine signaling 3 (SOCS3). SOCS3 knockdown experiments have previously demonstrated that short‐hairpin SOCS3‐BMSCs reversed the LIF suppressive effect. Our results demonstrated that LIF suppresses osteoblast differentiation through the LIF/STAT3/SOCS3 signaling pathway. J. Cell. Biochem. 115: 1262–1268, 2014. © 2014 Wiley Periodicals, Inc.
miR‐20a Promotes Prostate Cancer Invasion and Migration Through Targeting ABL2
Authors: Qiang Xiao‐Fei , Zhang Zheng‐Wei , Liu Qian , Sun Nan , Pan Liang‐Liang , Shen Jing , Li Tong , Yun Chen , Li Hui , Shi Li‐Hua 
Published: January 2014

ABSTRACTThe aberrant expression of microRNAs (miRNAs) has been found in various types of cancer. The present study found miR‐20a was significantly up‐regulated in prostate cancer compared with normal prostate tissues. Patients with a higher miR‐20a expression had a Gleason score of 7–10 and shorter survival time. The transwell and wound healing assays revealed that blocking expression of miR‐20a by miR‐20a ASO suppresses the invasion and migration of PC‐3 and DU145 cells in vitro and also inhibits tumor growth in vivo. Furthermore, we identified miR‐20a directly targets the ABL family non‐receptor tyrosine kinases ABL2 and negatively regulates the phosphorylation of its downstream gene p190RhoGAP. Knockdown of ABL2 promoted cell invasion and migration and we identified miR‐20a‐induced cell invasion and migration can be rescued by ABL2. In conclusion, our findings show that miR‐20a significantly contributes to the progression of prostate cancer by targeting ABL2. J. Cell. Biochem. 115: 1269–1276, 2014. © 2014 Wiley Periodicals, Inc.
Treatment of metalworking fluids using a submerged anaerobic membrane bioreactor (SAMBR)
Authors: Teli Aronne , Vyrides Ioannis , Stuckey David C. 
Published: January 2014

AbstractBACKGROUNDThe role of biodegradation and physical–chemical mechanisms involved in the treatment of metal working fluids (MWFs) using a submerged anaerobic membrane bioreactor (SAMBR) was assessed in this research. Two SAMBRs were employed in series, with the second SAMBR being fed the permeate from the first bioreactor.RESULTSResults showed a low biodegradability of the MWF both in batch tests (bioconversion efficiency ≤ 3%), and operating the bioreactors continuously (bioconversion efficiency ∼0%). Among the physical–chemical removal mechanisms identified, it was found that bio‐adhesion was the most important in MWF removal (removal rate of 96.0 ± 1.64%), whereas membrane rejection was not significant (removal rate of 0.2 ± 0.18%). However, it is expected that membrane separation will play an important role in removal after bio‐adhesion has been saturated.CONCLUSIONThe inhibition of the hydrolytic/heteroacetogenic biomass was the most problematic aspect in anaerobic digestion, resulting in the need for bioaugmentation of specific microorganisms to degrade MWFs. In the case of non‐adaptation during long‐term operation, the bio‐adhesive propensity of MWFs could be used as a potential method for removing spent MWFs from wastewater streams. © 2014 Society of Chemical Industry
In vivo absolute quantification for mouse muscle metabolites using an inductively coupled synthetic signal injection method and newly developed 1H/31P dual tuned probe
Authors: Lee Donghoon , Marro Kenneth , Mathis Mark , Shankland Eric , Hayes Cecil 
Published: January 2014

PurposeTo obtain robust estimates of 31P metabolite content in mouse skeletal muscles using our recently developed MR absolute quantification method and a custom‐built 1H/31P dual tuned radiofrequency (RF) coil optimized for mouse leg.Materials and MethodsWe designed and fabricated a probe consisting of two dual tuned 1H/31P solenoid coils: one leg was inserted to each solenoid. The mouse leg volume coil was incorporated with injector coils for MR absolute quantification. The absolute quantification method uses a synthetic reference signal injection approach and solves several challenges in MR absolute quantification including changes of coil loading and receiver gains.ResultsThe 1H/31P dual tuned probe was composed of two separate solenoid coils, one for each leg, to increase coil filling factors and signal‐to‐noise ratio. Each solenoid was equipped with a second coil to allow injection of reference signals. 31P metabolite concentrations determined for normal mice were well within the expected range reported in the literature.ConclusionWe developed an RF probe and an absolute quantification approach adapted for mouse skeletal muscle. J. Magn. Reson. Imaging 2014;39:1039–1046. © 2014 Wiley Periodicals, Inc.
The Enigma of Čiurlionis's Illness and Its Relationship to His Creativity
Authors: Lerner Vladimir , Witztum Eliezer 
Published: January 2014

AbstractAssumption regarding the relationship between creativity and mental disturbances has attracted academic and public interest from antiquity. Research performed in recent years, support these associations and show a disproportionately high rate of mental illnesses, especially bipolar disorder, in creative individuals. In this article, we give another example of this link by describing the life and works of a Lithuanian composer, painter, and poet Mikalojus Konstantinas Chiurlionis (1875–1911) (Lithuanian transcription—Čiurlionis). This relationship is demonstrated graphically, which enables to see a link between his mood fluctuations and quantity of Chiurlionis' works. Chiurlionis lived in the complex period with its turbulent whirl of changing trends in art doubtlessly influenced his art. Chiurlionis had synesthesia and he was one of the first who tried to join music and painting in a synthesis of the arts. Significant components of his work are related to Symbolism. Chiurlionis lived very short life—only 36 years, but during his life, he created over 200 musical works, and painted about 300 canvases. His compositions were romantic and nationalist in spirit, sometimes inspired by Lithuanian folklore and his best work is thought to be his “musical paintings”. For many years, they were virtually unknown outside his motherland. While it was well‐known that Chiurlionis died in a sanatorium for mental ill, almost nothing was written about his mental illness. The purpose of this study was to demonstrate that the Chiurlionis' illness (bipolar affective disorder) and his creative output in music and paintings were connected.
CEOIdeational Facilitation Leadership and Team Creativity: The Mediating Role of Knowledge Sharing
Authors: Carmeli Abraham , Paulus Paul B. 
Published: January 2014

AbstractThe development of new ventures is often based on collective creative efforts. We conceptualize team creativity as a process of looking for and exploring new solutions and examine whether and howCEOleadership fosters creativity in top management teams (TMT). Data collected from senior executive teams indicate thatCEOideational facilitation leadership is positively related to team knowledge sharing, which in turn results in enhanced team exploratory behaviors. These findings bear important theoretical implications for ways in which leaders can enhance creativity in teams and augment the entrepreneurial direction of executive teams.
NUCB2/nesfatin‐1: A new adipokine expressed in human and murine chondrocytes with pro‐inflammatory properties, an in vitro study
Authors: Scotece Morena , Conde Javier , Abella Vanessa , López Veronica , Lago Francisca , Pino Jesús , Gómez‐Reino Juan J. , Gualillo Oreste 
Published: January 2014

ABSTRACTNesfatin‐1 is a recently discovered satiety‐inducing adipokine identified in hypothalamic regions that regulates energy balance. So far, no data exist on NUCB2/nesfatin‐1 localization in human and murine chondrocytes. Here, we therefore investigated NUCB2/nesfatin‐1 gene and protein expression in human and murine chondrocytes and the effect of nesfatin‐1 on pro‐inflammatory cytokines expression. Peptide localization was performed by laser confocal microscopy, NUCB2 mRNA expression was studied by RT‐PCR and protein secretion was measured by XMap technology and Western blot analysis. First, we demonstrated cytoplasmic localization of NUCB2/nesfatin‐1 peptide in both human and murine chondrocytes. We present evidence that both mRNA and protein expression of NUCB2 were increased during the differentiation of ATDC5 murine chondrocyte cell line. Furthermore, we demonstrated that nesfatin‐1 induces IL‐6 and MIP‐1α mRNA expression and protein secretion in ATDC‐5 cells challenged with IL‐1, and also increases COX‐2 mRNA expression in these cells. Finally, nesfatin‐1 provoked a clear induction of pro‐inflammatory agents, such as COX‐2, IL‐8, IL‐6, and MIP‐1α in human primary chondrocytes from OA patients. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:653–660, 2014.
Disruption of the anterior–posterior rotator cuff force balance alters joint function and leads to joint damage in a rat model
Authors: Reuther Katherine E. , Thomas Stephen J. , Tucker Jennica J. , Sarver Joseph J. , Gray Chancellor F. , Rooney Sarah I. , Glaser David L. , Soslowsky Louis J. 
Published: January 2014

ABSTRACTThe rotator cuff assists in shoulder movement and provides dynamic stability to the glenohumeral joint. Specifically, the anterior–posterior (AP) force balance, provided by the subscapularis anteriorly and the infraspinatus and teres minor posteriorly, is critical for joint stability and concentric rotation of the humeral head on the glenoid. However, limited understanding exists of the consequences associated with disruption of the AP force balance (due to tears of both the supraspinatus and infraspinatus tendons) on joint function and joint damage. We investigated the effect of disrupting the APforce balance on joint function and joint damage in an overuse rat model. Twenty‐eight rats underwent 4 weeks of overuse to produce a tendinopathic condition and were then randomized into two surgical groups: Detachment of the supraspinatus only or detachment of the supraspinatus and infraspinatus tendons. Rats were then gradually returned to their overuse protocol. Quantitative ambulatory measures including medial/lateral, propulsion, braking, and vertical forces were significantly different between groups. Additionally, cartilage and adjacent tendon properties were significantly altered. These results identify joint imbalance as a mechanical mechanism for joint damage and demonstrate the importance of preserving rotator cuff balance when treating active cuff tear patients. © 2014 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 32:638–644, 2014.
Psychometric Properties of the Child PTSD Symptom Scale in Latino Children
Authors: Gudiño Omar G. , Rindlaub Laura A. 
Published: January 2014

AbstractThe Child PTSD Symptom Scale (Foa, Johnson, Feeny, & Treadwell, ) is a self‐report measure of posttraumatic stress disorder symptoms (PTSD) in children and adolescents. Despite widespread use of this measure, no study to our knowledge has examined its psychometric properties in Latino children. This study examined the factor structure, internal consistency, and convergent validity of the measure utilizing a sample of 161 Latino students (M = 11.42 years, SD = 0.70) at high risk of exposure to community violence. Confirmatory factor analyses suggested that a 3‐factor model consistent with the Diagnostic and Statistical Manual of Mental Disorders (4th ed., text rev.; DSM‐IV‐TR; American Psychiatric Association, ) provided the best fit to the data. Internal consistency of the total scale and subscales was high when completed in English or Spanish. All Child PTSD Symptom Scale scores were positively correlated with violence exposure. As additional evidence of convergent validity, scores evidenced stronger correlations with internalizing symptoms than with externalizing symptoms. Results supported the use of the Child PTSD Symptom Scale as a measure of PTSD severity in Latino children, but additional research is needed to determine appropriate clinical cutoffs for Latino youths exposed to chronic levels of violence. Implications for clinical practice and future research are discussed.