bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epizootic Neoplasia of the Lateral Line System of Lake Trout (Salvelinus namaycush) in New York’s Finger Lakes
Authors: Spitsbergen J. M. , Frattini S. A. , Bowser P. R. , Getchell R. G. , Coffee L. L. , Wolfe M. J. , Fisher J. P. , Marinovic S. J. , Harr K. E. 
Published: March 2013

This article documents an epizootic of inflammation and neoplasia selectively affecting the lateral line system of lake trout ( Salvelinus namaycush) in 4 Finger Lakes in New York from 1985 to 1994. We studied more than 100 cases of this disease. Tumors occurred in 8% (5/64) of mature and 21% (3/14) of immature lake trout in the most severely affected lake. Lesions consisted of 1 or more neoplasm(s) in association with lymphocytic inflammation, multifocal erosions, and ulcerations of the epidermis along the lateral line. Lesions progressed from inflammatory to neoplastic, with 2-year-old lake trout showing locally extensive, intense lymphocytic infiltrates; 2- to 3-year-old fish having multiple, variably sized white masses up to 3 mm in diameter; and fish over 5 years old exhibiting 1 or more white, cerebriform masses greater than 1 cm in diameter. Histologic diagnoses of the tumors were predominantly spindle cell sarcomas or benign or malignant peripheral nerve sheath neoplasms, with fewer epitheliomas and carcinomas. Prevalence estimates did not vary significantly between sexes or season. The cause of this epizootic remains unclear. Tumor transmission trials, virus isolation procedures, and ultrastructural study of lesions failed to reveal evidence of a viral etiology. The Finger Lakes in which the disease occurred did not receive substantially more chemical pollution than unaffected lakes in the same chain during the epizootic, making an environmental carcinogen an unlikely primary cause of the epizootic. A hereditary component, however, may have contributed to this syndrome since only fish of the Seneca Lake strain were affected.
Antimutagenic and genoprotective effects of Saraca asoca bark extract
Authors: Nag Debarati , Ghosh Manosij , Mukherjee Anita 
Published: March 2013

Aim of the study: Traditionally herbal formulations have been used effectively for the management of disorders that are now being accepted worldwide. Saraca asoca bark extract (SAE) finds use in traditional herbal medicine. In the present study, SAE were studied for their antioxidant, antimutagenic, and antigenotoxic properties. Methods: SAE were evaluated for antimutagenic property in Salmonella strains (TA97a, TA98, TA100, and TA102), in the presence and absence of metabolic activation (S9). The SAE was also studied for antigenotoxic property against cyclophosphamide (CP) in Swiss albino male mice in vivo. The extract was analyzed using high-performance liquid chromatography (HPLC). Results: The study reveals antimutagenic property of the bark extract in Salmonella strains in the presence and absence of metabolic activation (S9). The study reports antigenotoxic property of the bark extract against CP in vivo. Thiobarbituric acid reactive species assay on the bark extract revealed antioxidant property. HPLC revealed the presence of two peaks corresponding to gallic acid and (−)-epicatechin, respectively. Conclusion: The study clearly reveals the antimutagenic and antigenotoxic properties of SAE.
Percutaneous Intraperitoneal Catheters in Neonates Following Open Heart Surgery
Authors: Averbuch Noa , Birk Einat , Frenkel Georgy , Gogia Omar , Shulman Orit Manor , Bruckheimer Elchanan , Nachum Elchanan , Amir Gabriel 
Published: March 2013

Objective: Ascitic fluid in the peritoneal cavity may severely impair respiratory and renal function following neonatal heart surgery. It has been our practice to liberally insert percutaneous peritoneal catheters (PPCs) in order to manage fluid balance and thereby improve ventilatory function. We herein report our experience with PPC. Methods: Retrospective analysis of charts of all surviving neonates that underwent PPC insertion from January 2007 through March 2010. Charts were reviewed for demographic and clinical variables from the preoperative, operative, and postoperative periods. Results: A total of 1268 patients underwent surgery, 292 (23%) were neonates. 17 (5.8%) patients required PPC. Mean age and weight were 16 days and 3.1 kg, respectively. Mean amount drained upon insertion was 55 ± 46 ml. Catheters were maintained for a mean of 5 days and drained an average of 201 ml on the first postinsertion day. Ventilatory settings did not change significantly prior to and postcatheter insertion (respiratory rate [29 ± 3.8 vs 28.7 ± 3.9; P = .93], inspiratory pressures [26.3 ± 3.6 vs 26.1 ± 3.3 cm H2O; P = .34], and fraction of inspired oxygen [0.66 ± 0.21 vs 0.63 ± 0.18; P = .53]). Carbon dioxide values decreased significantly (43.2 ± 9.7 vs 37 ± 4.9 mm Hg; P = .01), and PO2 values increased (78 ± 69 vs 104 ± 57 mm Hg; P = .05). Conclusions: The PPC insertion can be easily performed at the bedside with minimal complications. Fluid balance management is facilitated, and ventilation is improved. The PPC insertion is a valuable addition to the armamentarium of the physician treating neonates in the intensive care unit after complex congenital heart surgery.
Evaluating change in mobility in people with multiple sclerosis: relative responsiveness of four clinical measures
Authors: Freeman Jennifer , Walters Richard , Ingram Wendy , Slade Anita , Hobart Jeremy , Zajicek John 
Published: March 2013

Background and objectives: Determining responsiveness of measures across different samples and settings is important for selecting measures of mobility and understanding multiple sclerosis (MS) study results. Currently such information is limited. Methods: This exploratory study examined the relative responsiveness of four mobility measures (walking velocity, 6-minute walk distance, Rivermead Mobility Index and MS Walking Scale) in a community sample ( n = 70), after three annual assessments. Distribution based estimates and anchor-based methods (comparison against transition questions) determined responsiveness. A head-to-head comparison was made. Results: While variations in individuals occurred, the group mean change scores for all measures was small, suggesting that there was minimal deterioration in the total sample. Consistent with this, total sample Effect Size (ES) was negligible to small (ES −0.32 to +0.03) for all measures. Differentiation between sub-groups, defined by the participants’ perception of change in mobility over the past year (transition questions), showed that some instruments could detect clinically significant changes (small sample sizes limited this interpretation). Correlation analyses between change scores demonstrated that these measures captured related, but different information ( r < 0.364). Conclusions: The measures were broadly comparable in detecting mobility changes in this community sample. These correlations highlight that in selection of measures, one should also consider the discrete mobility dimension that the intervention intends to impact.
Susceptibility for Depression in Current and Retired Student Athletes
Authors: Weigand Sabrina , Cohen Jared , Merenstein Daniel 
Published: March 2013

Background: Depression, a disabling mental disorder, adversely affects work, sleeping and eating habits, and family. Research does not exist on depression among athletes who have recently graduated from college and retired from their sport after exhausting their collegiate eligibility. Hypothesis: Changes in lifestyle and loss of personal identity, which follow college athletics, would put former college athletes at an increased risk for depression. Methods: A survey was sent to former (n = 163) and current (n = 117) college athletes to correlate depression and retirement from athletics. Results: Depression levels were significantly higher ( P = 0.03) in current college athletes (16.77%, n = 27) compared with former, graduated college athletes (8.03%, n = 9). Conclusion: Completion of college sports may not increase levels of depression. There is a need for increased awareness, education, screening, and intervention for depression in college athletes. Clinical Relevance: This study suggests that student athletes’ depression levels should be monitored during their participation in college sports.
The six-hour rule for testis fixation in testicular torsion: is it history?
Authors: Hadway Paul , Reynard John M 
Published: March 2013

Objectives: To explore the historical and recent evidence behind the urological rule that operative management within six hours of pain onset in testicular torsion is necessary for organ salvage To use this information to guide those involved in related medico-legal cases Methods: Focused search of the PubMed database. Translation from German to English of the sentinel work of Hellner from 1933. Results: Hellner’s canine studies showed that complete ischaemia of the testis beyond six hours resulted in necrosis and then atrophy. He also showed that the degree of torsion was a key determinant of outcome with organ salvage possible at up to 24 hours when the twist was less than 3600. Recent clinical series support these findings and suggest a success rate of ≥98% when exploration is performed within six hours. Testicular salvage may be as good as 89% when surgery is delayed to 12 hours. Related medico-legal cases may consider whether the operative delay was causative in testicular loss, and where liability may lie for any such delay. Specific literature in this area is sparse. Conclusion: Hellner’s work explains the basis of the six hour rule and its application remains valid in today’s clinical practice. Surgical exploration is indicated out to 24 hours, however, given that testes can still be saved and that the degree of torsion cannot be gleaned pre-operatively. Medico-legally, we believe that an operative delay of up to 8 hours is probably safe when considering the causation of testicular loss, but more data are needed. Liability is more complex in that many factors can contribute to the delay and may be beyond the control of the doctor or hospital.
EZH2 overexpression in natural killer/T-cell lymphoma confers growth advantage independently of histone methyltransferase activity
Authors: Yan Junli , Ng Siok-Bian , Tay Jim Liang-Seah , Lin Baohong , Koh Tze Loong , Tan Joy , Selvarajan Viknesvaran , Liu Shaw-Cheng , Bi Chonglei , Wang Shi , Choo Shoa-Nian , Shimizu Norio , Huang Gaofeng , Yu Qiang , Chng Wee-Joo 
Published: March 2013

Key Points
This study has uncovered an oncogenic role of EZH2 independent of its methyltransferase activity in NKTL. This study suggests that targeting EZH2 may have therapeutic usefulness in NKTL.
Self-reported headache among the employees of a Swiss university hospital: prevalence, disability, current treatment, and economic impact
Authors: Sokolovic Emina , Riederer Franz , Szucs Thomas , Agosti Reto , Sándor Peter Stefan 
Published: March 2013

Abstract

Background
The objectives of this cross-sectional, observational study were to determine the prevalence of self-reported headache among the employees of the large Swiss university hospital, to measure the impact of headache using the MIDAS questionnaire, to assess current treatment and to estimate economic burden of headache considering indirect costs.

Methods
A self-administered questionnaire was distributed internally to 2000 randomly selected employees of the University Hospital Zurich.

Results
1210 employees (60.5%) responded. Of the 1192 (98.5%) employees who provided sufficiently complete information, 723 (61%) reported at least one headache type in the last three months. The prevalence of migraine, and tension-type headache was 20% and 50%, respectively. Regarding the occupational groups, there was a trend that healthcare staff, administration employees, and medical technicians suffered more from headaches than physicians, correcting for age and sex. The economic consequences of lost productivity were calculated to amount to approximately 14 million Swiss Francs (9.5 million EUR), representing 3.2% of the overall annual expenditure of the hospital for personnel.

Conclusion
Headache is highly prevalent among university hospital employees, with significant economic impact.
Genotype-phenotype matching analysis of 38 Lactococcus lactisstrains using random forest methods
Authors: Bayjanov Jumamurat R , Starrenburg Marjo JC , van der Sijde Marijke R , Siezen Roland J , van Hijum Sacha AFT 
Published: March 2013

Abstract

Background

Lactococcus lactis is used in dairy food fermentation and for the efficient production of industrially relevant enzymes. The genome content and different phenotypes have been determined for multiple L. lactis strains in order to understand intra-species genotype and phenotype diversity and annotate gene functions. In this study, we identified relations between gene presence and a collection of 207 phenotypes across 38 L. lactis strains of dairy and plant origin. Gene occurrence and phenotype data were used in an iterative gene selection procedure, based on the Random Forest algorithm, to identify genotype-phenotype relations.

Results
A total of 1388 gene-phenotype relations were found, of which some confirmed known gene-phenotype relations, such as the importance of arabinose utilization genes only for strains of plant origin. We also identified a gene cluster related to growth on melibiose, a plant disaccharide; this cluster is present only in melibiose-positive strains and can be used as a genetic marker in trait improvement. Additionally, several novel gene-phenotype relations were uncovered, for instance, genes related to arsenite resistance or arginine metabolism.

Conclusions
Our results indicate that genotype-phenotype matching by integrating large data sets provides the possibility to identify gene-phenotype relations, possibly improve gene function annotation and identified relations can be used for screening bacterial culture collections for desired phenotypes. In addition to all gene-phenotype relations, we also provide coherent phenotype data for 38 Lactococcus strains assessed in 207 different phenotyping experiments, which to our knowledge is the largest to date for the Lactococcus lactis species.
Type IV collagen stimulates pancreatic cancer cell proliferation, migration, and inhibits apoptosis through an autocrine loop
Authors: Öhlund Daniel , Franklin Oskar , Lundberg Erik , Lundin Christina , Sund Malin 
Published: March 2013

Abstract

Background
Pancreatic cancer shows a highly aggressive and infiltrative growth pattern and is characterized by an abundant tumor stroma known to interact with the cancer cells, and to influence tumor growth and drug resistance. Cancer cells actively take part in the production of extracellular matrix proteins, which then become deposited into the tumor stroma. Type IV collagen, an important component of the basement membrane, is highly expressed by pancreatic cancer cells both in vivo and in vitro. In this study, the cellular effects of type IV collagen produced by the cancer cells were characterized.

Methods
The expression of type IV collagen and its integrin receptors were examined in vivo in human pancreatic cancer tissue. The cellular effects of type IV collagen were studied in pancreatic cancer cell lines by reducing type IV collagen expression through RNA interference and by functional receptor blocking of integrins and their binding-sites on the type IV collagen molecule.

Results
We show that type IV collagen is expressed close to the cancer cells in vivo, forming basement membrane like structures on the cancer cell surface that colocalize with the integrin receptors. Furthermore, the interaction between type IV collagen produced by the cancer cell, and integrins on the surface of the cancer cells, are important for continuous cancer cell growth, maintenance of a migratory phenotype, and for avoiding apoptosis.

Conclusion
We show that type IV collagen provides essential cell survival signals to the pancreatic cancer cells through an autocrine loop.
53BP1 expression is a modifier of the prognostic value of lymph node ratio and CA 19–9 in pancreatic adenocarcinoma
Authors: Ausborn Natalie L , Wang Tong , Wentz Sabrina C , Washington Mary Kay , Merchant Nipun B , Zhao Zhiguo , Shyr Yu , Chakravarthy Anuradha Bapsi , Xia Fen 
Published: March 2013

AbstractBackground53BP1 binds to the tumor suppressor p53 and has a key role in DNA damage response and repair. Low 53BP1 expression has been associated with decreased survival in breast cancer and has been shown to interact with several prognostic factors in non-small cell lung cancer. The role of 53BP1 in pancreatic ductal adenocarcinoma (PDAC) has yet to be determined. We aimed to investigate whether 53BP1 levels interact with established prognostic factors in PDAC.Methods106 patients for whom there was tissue available at time of surgical resection for PDAC were included. A tissue microarray was constructed using surgical specimens, stained with antibodies to 53BP1, and scored for expression intensity. Univariate and multivariate statistical analyses were performed to investigate the association between 53BP1 and patient survival with known prognostic factors for survival.ResultsThe association of 53BP1 with several established prognostic factors was examined, including stage, tumor grade, surgical margin, peripancreatic extension, lymph node ratio (LNR), and CA 19–9. We found that 53BP1 modified the effects of known prognostic variables including LNR and CA 19–9 on survival outcomes. When 53BP1 intensity was low, increased LNR was associated with decreased OS (HR 4.84, 95% CI (2.26, 10.37),p<0.001) and high CA19-9 was associated with decreased OS (HR 1.72, 95% CI (1.18, 2.51),p=0.005). When 53BP1 intensity was high, LNR and CA19-9 were no longer associated with OS (p=0.958 andp=0.606, respectively).ConclusionsIn this study, 53BP1, a key player in DNA damage response and repair, was found to modify the prognostic value of two established prognostic factors, LNR and CA 19–9, suggesting 53BP1 may alter tumor behavior and ultimately impact how we interpret the value of other prognostic factors.
Epigenetic regulation of L1CAM in endometrial carcinoma: comparison to cancer–testis (CT-X) antigens
Authors: Schirmer Uwe , Fiegl Heidi , Pfeifer Marco , Zeimet Alain G , Müller-Holzner Elisabeth , Bode Peter K , Tischler Verena , Altevogt Peter 
Published: March 2013

Abstract

Background
L1CAM was originally identified as an adhesion molecule involved in neural development. In many human carcinomas L1CAM is over-expressed and is associated with a bad prognosis. We previously reported that L1CAM was absent in the vast majority of endometrioid endometrial carcinomas (ECs) (type 1) but was strongly expressed in the more aggressive serous and clear-cell ECs (termed type 2). The differential regulation of L1CAM in ECs is not well understood. Recent evidence suggests that it can be regulated by epigenetic mechanisms. Here we investigated the role of DNA-methylation of the L1CAM promoter for expression. We also studied the relationship to cancer testis (CT-X) antigens that co-localize with L1CAM on chromosome Xq28, a region that is often activated in human tumors.

Methods
We used EC cell lines and primary tumor tissues for our analysis. For expression analysis we employed RT-PCR and Western blotting. DNA-Methylation of the L1CAM promoter was determined after bisulfite conversation and DNA sequencing. Tumor tissues were examined by immunohistochemical (IHC) staining.

Results
We demonstrate that the treatment of L1CAM low/negative expressing EC cell lines with 5′-Azacytidine (5-AzaC) or knock-down of DNMT1 (DNA methyltransferase 1) as well as the HDAC (histone deacetylase) inhibitor Trichostatin A (TSA) up-regulated L1CAM at the mRNA and protein level. The L1CAM gene has two promoter regions with two distinct CpG islands. We observed that the expression of L1CAM correlated with hypermethylation in promoter 1 and 5-AzaC treatment affected the DNA-methylation pattern in this region. The CT-X antigens NY-ESO-1, MAGE-A3 and MAGE-A4 were also strongly up-regulated by 5-AzaC or knock-down of DNMT1 but did not respond to treatment with TSA. Primary EC tumor tissues showed a variable methylation pattern of the L1CAM promoter. No striking differences in promoter methylation were observed between tumor areas with L1CAM expression and those without expression.

Conclusions
L1CAM expression correlated with methylation of the L1CAM promoter in EC cell lines. In negative cell lines L1CAM expression is up-regulated by epigenetic mechanism. Although genes localized on Xq28 are often re-expressed by human tumors, L1CAM and CT-X antigens show distinct regulation in response to HADC inhibitors and 5-AzaC.
Development of a green binder system for paper products
Authors: Flory Ashley R , Vicuna Requesens Deborah , Devaiah Shivakumar P , Teoh Keat Thomas , Mansfield Shawn D , Hood Elizabeth E 
Published: March 2013

AbstractBackgroundIt is important for industries to find green chemistries for manufacturing their products that have utility, are cost-effective and that protect the environment. The paper industry is no exception. Renewable resources derived from plant components could be an excellent substitute for the chemicals that are currently used as paper binders. Air laid pressed paper products that are typically used in wet wipes must be bound together so they can resist mechanical tearing during storage and use. The binders must be strong but cost-effective. Although chemical binders are approved by the Environmental Protection Agency, the public is demanding products with lower carbon footprints and that are derived from renewable sources.ResultsIn this project, carbohydrates, proteins and phenolic compounds were applied to air laid, pressed paper products in order to identify potential renewable green binders that are as strong as the current commercial binders, while being organic and renewable. Each potential green binder was applied to several filter paper strips and tested for strength in the direction perpendicular to the cellulose fibril orientation. Out of the twenty binders surveyed, soy protein, gelatin, zein protein, pectin andSalixlignin provided comparable strength results to a currently employed chemical binder.ConclusionsThese organic and renewable binders can be purchased in large quantities at low cost, require minimal reaction time and do not form viscous solutions that would clog sprayers, characteristics that make them attractive to the non-woven paper industry. As with any new process, a large-scale trial must be conducted along with an economic analysis of the procedure. However, because multiple examples of “green” binders were found that showed strong cross-linking activity, a candidate for commercial application will likely be found.