bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting c-MYC in Platinum-Resistant Ovarian Cancer
Authors: Reyes-González Jeyshka M. , Armaiz-Peña Guillermo N. , Mangala Lingegowda S. , Valiyeva Fatma , Ivan Cristina , Pradeep Sunila , Echevarría-Vargas Ileabett M. , Rivera-Reyes Adrian , Sood Anil K. , Vivas-Mejía Pablo E. 
Published: July 2015

Abstract
The purpose of this study was to investigate the molecular and therapeutic effects of siRNA-mediated c-MYC silencing in cisplatin-resistant ovarian cancer. Statistical analysis of patient's data extracted from The Cancer Genome Atlas (TCGA) portal showed that the disease-free (DFS) and the overall (OS) survival were decreased in ovarian cancer patients with high c-MYC mRNA levels. Furthermore, analysis of a panel of ovarian cancer cell lines showed that c-MYC protein levels were higher in cisplatin-resistant cells when compared with their cisplatin-sensitive counterparts. In vitro cell viability, growth, cell-cycle progression, and apoptosis, as well as in vivo therapeutic effectiveness in murine xenograft models, were also assessed following siRNA-mediated c-MYC silencing in cisplatin-resistant ovarian cancer cells. Significant inhibition of cell growth and viability, cell-cycle arrest, and activation of apoptosis were observed upon siRNA-mediated c-MYC depletion. In addition, single weekly doses of c-MYC–siRNA incorporated into 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG-2000)-based nanoliposomes resulted in significant reduction in tumor growth. These findings identify c-MYC as a potential therapeutic target for ovarian cancers expressing high levels of this oncoprotein. Mol Cancer Ther; 14(10); 2260–9. ©2015 AACR.
Cytokeratin-20 and Survivin-Expressing Circulating Tumor Cells Predict Survival in Metastatic Colorectal Cancer Patients by a Combined Immunomagnetic qRT-PCR Approach
Authors: Ning Yan , Hanna Diana L. , Zhang Wu , Mendez Angela , Yang Dongyun , El-Khoueiry Rita , Matsusaka Satoshi , Sunakawa Yu , Stremitzer Stefan , Parekh Anish , Okazaki Satoshi , Berger Martin D. , Barzi Afsaneh , Lenz Heinz-Josef 
Published: July 2015

Abstract
Circulating tumor cells (CTC) express epithelial and stem cell–like genes, though current approved detection methods mainly use epithelial markers. We optimized a CTC isolation method that could capture their molecular heterogeneity and predict overall survival (OS) in metastatic colorectal cancer (mCRC) patients receiving various chemotherapy regimens. We combined immunomagnetic enrichment of CD45-negative, EpCAM-positive circulating cancer cells with qRT-PCR amplification of CK20 and survivin expression in 88 mCRC patients and 20 healthy controls. We then evaluated the prognostic value of baseline CTC CK20 and survivin expression in mCRC patients. The presence of elevated CTC CK20 or survivin expression distinguished mCRC patients from controls with sufficient sensitivity (79.6%) and specificity (85%). In univariate analysis, patients with high CTC-CK20 expression (9 vs. 33.2+ months, log-rank P &lt; 0.001) or high CTC-survivin expression (10 vs. 33.2+ months, log-rank P = 0.032) had a significantly worse median OS than those with low expression of either marker. In multivariable analysis, the high CTC-CK20 group had significantly shortened OS (HR, 3.11; adjusted P = 0.01), and there was a trend toward inferior OS in the high CTC-survivin group (HR, 1.76; adjusted P = 0.099). Patients with either high CTC CK20 or survivin expression had inferior OS compared with those with low expression of both markers (HR, 4.39; 95% confidence interval, 1.56–12.35; adjusted P = 0.005). Colorectal cancer CTCs can be reliably isolated using epithelial and stem cell markers. CTC CK20 and survivin expression may effectively predict OS in mCRC patients receiving chemotherapy. Mol Cancer Ther; 14(10); 2401–8. ©2015 AACR.
A Novel Approach to Diagnosing Southern Hemisphere Planetary Wave Activity and Its Influence on Regional Climate Variability
Authors: Irving Damien , Simmonds Ian 
Published: July 2015

Abstract
Southern Hemisphere mid- to upper-tropospheric planetary wave activity is characterized by the superposition of two zonally oriented, quasi-stationary waveforms: zonal wavenumber 1 (ZW1) and zonal wavenumber 3 (ZW3). Previous studies have tended to consider these waveforms in isolation and with the exception of those studies relating to sea ice, little is known about their impact on regional climate variability. A novel approach is taken to quantifying the combined influence of ZW1 and ZW3, using the strength of the hemispheric meridional flow as a proxy for zonal wave activity. The methodology adapts the wave envelope construct routinely used in the identification of synoptic-scale Rossby wave packets and improves on existing approaches by allowing for variations in both wave phase and amplitude. While ZW1 and ZW3 are both prominent features of the climatological circulation, the defining feature of highly meridional hemispheric states is an enhancement of the ZW3 component. Composites of the mean surface conditions during these highly meridional, ZW3-like anomalous states (i.e., months of strong planetary wave activity) reveal large sea ice anomalies over the Amundsen and Bellingshausen Seas during autumn and along much of the East Antarctic coastline throughout the year. Large precipitation anomalies in regions of significant topography (e.g., New Zealand, Patagonia, and coastal Antarctica) and anomalously warm temperatures over much of the Antarctic continent were also associated with strong planetary wave activity. The latter has potentially important implications for the interpretation of recent warming over West Antarctica and the Antarctic Peninsula.
Is MRI Necessary in the Evaluation of Pediatric Central Sleep Apnea?
Authors: Woughter Meghan , Perkins Amy M. , Baldassari Cristina M. 
Published: July 2015

Objectives(1) To determine the prevalence of central nervous system (CNS) pathology identified on head magnetic resonance imaging (MRI) scans in children with central sleep apnea (CSA); (2) to assess the yield of MRI in evaluation of CSA; and (3) to identify factors that predict CNS pathology in children with CSA.Study DesignCase series with chart review.SettingTertiary children’s hospital.Subjects and MethodsA chart review was conducted over 12 years. Patients 6 months to 18 years of age who underwent head MRI for evaluation of CSA were included. CSA was diagnosed on polysomnogram as central apnea index >1.ResultsForty children were included in the CSA group. Twenty‐two patients were male, and the mean age was 60 ± 41.5 months. The mean central apnea index was 3.8 ± 1.9, while the mean obstructive apnea hypopnea index was 3.4 (interquartile range, 0.7‐3.8). Eighteen percent (7 of 40) of children with CSA had evidence of CNS pathology on MRI, with the most common finding (n = 3) being arachnoid cyst. Children with CSA who had gastroesophageal reflux disease or abnormal neurologic examinations were more likely to have CNS pathology. Other factors, such as prematurity, did not improve the yield of MRI in children with CSA.ConclusionsWhile routine evaluation of children with elevated central apnea index by MRI is not indicated, providers should consider neuroimaging in children with CSA and abnormal neurologic examination findings or gastroesophageal reflux disease. Further research is necessary to identify other tests with improved diagnostic yield for evaluation of pediatric CSA.
Risk factors for endovenous heat-induced thrombosis after endovenous radiofrequency ablation performed in Thailand
Authors: Sermsathanasawadi Nuttawut , Voravitvet Tsz Yin , Chinsakchai Khamin , Wongwanit Chumpol , Ruangsetakit Chanean , Mutirangura Pramook 
Published: August 2015

Objective We aimed to determine the incidence of and associated risk factors for endovenous heat-induced thrombosis (EHIT) after endovenous radiofrequency ablation (RFA). Methods We retrospectively reviewed the medical records of 82 patients with 97 great saphenous veins undergoing RFA from 2012 to 2014. Results The incidence of EHIT was 10.3%. Class 1, 2, and 3 EHIT was found in 50%, 30%, and 20% of legs, respectively. No class 4 EHIT, deep vein thrombosis, or pulmonary emboli occurred. Univariate analysis revealed that the associated risk factors for EHIT were a vein diameter of >10 mm, operative time of >40 min, and Caprini score of >6. Multivariate analysis revealed that the independent risk factors associated with EHIT were a vein diameter of >10 mm and operative time of >40 min. Conclusions A vein diameter of >10 mm and operative time of >40 min might be predictive factors for EHIT following RFA.
Coverage and development of specialist palliative care services across the World Health Organization European Region (2005–2012): Results from a European Association for Palliative Care Task Force survey of 53 Countries
Authors: Centeno Carlos , Lynch Thomas , Garralda Eduardo , Carrasco José Miguel , Guillen-Grima Francisco , Clark David 
Published: August 2015

Background: The evolution of the provision of palliative care specialised services is important for planning and evaluation. Aim: To examine the development between 2005 and 2012 of three specialised palliative care services across the World Health Organization European Region – home care teams, hospital support teams and inpatient palliative care services. Design and setting: Data were extracted and analysed from two editions of the European Association for Palliative Care Atlas of Palliative Care in Europe. Significant development of each type of services was demonstrated by adjusted residual analysis, ratio of services per population and 2012 coverage (relationship between provision of available services and demand services estimated to meet the palliative care needs of a population). For the measurement of palliative care coverage, we used European Association for Palliative Care White Paper recommendations: one home care team per 100,000 inhabitants, one hospital support team per 200,000 inhabitants and one inpatient palliative care service per 200,000 inhabitants. To estimate evolution at the supranational level, mean comparison between years and European sub-regions is presented. Results: Of 53 countries, 46 (87%) provided data. Europe has developed significant home care team, inpatient palliative care service and hospital support team in 2005–2012. The improvement was statistically significant for Western European countries, but not for Central and Eastern countries. Significant development in at least a type of services was in 21 of 46 (46%) countries. The estimations of 2012 coverage for inpatient palliative care service, home care team and hospital support team are 62%, 52% and 31% for Western European and 20%, 14% and 3% for Central and Eastern, respectively. Conclusion: Although there has been a positive development in overall palliative care coverage in Europe between 2005 and 2012, the services available in most countries are still insufficient to meet the palliative care needs of the population.
Clinical presentation and CT/MRI findings of alveolar soft part sarcoma: a retrospective single-center analysis of 14 cases
Authors: Tian Li , Cui Chun-Yan , Lu Su-Ying , Cai Pei-Qiang , Xi Shao-Yan , Fan Wei 
Published: August 2015

BackgroundAlveolar soft part sarcoma (ASPS) is a relatively rare malignant tumor and early diagnosis and appropriate treatment for ASPS are essential for a good prognosis.PurposeTo retrospectively review the clinical presentation and computed tomography (CT) and magnetic resonance imaging (MRI) findings of ASPS so as to improve the accuracy of imaging diagnosis.Material and MethodsFourteen patients with pathologically proven ASPS were enrolled. Their clinical and imaging findings were retrospectively reviewed.ResultsThe median age of the patients was 29 years (range, 13–37 years). Most tumors were located in the soft tissues of the trunk and lower limbs. The median maximal diameter of the masses was 91 mm. Thirteen masses presented with ovoid or irregular shapes. Eleven masses had less clear boundaries. Compared with the adjacent muscles, the masses were isodense or hypodense on CT, hypo-, iso-, or hyperintense on T1-weighted images, and heterogeneous hyperintense on T2-weighted images. Intense enhancement was seen after contrast agent administration, with prominent intra- or peri-tumoral feeders on CT or flow voids on MRI. By the end of the last follow-up, 13 patients had distant metastasis and three patients had local recurrence.ConclusionASPS should be included in the differential diagnosis when a bulky, heterogeneous soft tissue mass in the trunk and the lower limbs with intense enhancement after contrast administration and prominent intra- or peri-tumoral feeders on CT or flow voids on MRI is seen, particularly in young patients.
Pilot study of sphenopalatine injection of onabotulinumtoxinA for the treatment of intractable chronic cluster headache
Authors: Bratbak Daniel Fossum , Nordgård Ståle , Stovner Lars Jacob , Linde Mattias , Folvik Mari , Bugten Vegard , Tronvik Erling 
Published: August 2015

Objective The main object of this pilot study was to investigate the safety of administering onabotulinumtoxinA (BTA) towards the sphenopalatine ganglion (SPG) in intractable chronic cluster headache. Efficacy data were also collected to provide indication on whether future placebo-controlled studies should be performed. Method In a prospective, open-label, uncontrolled study, we performed a single injection of 25 IU ( n = 5) or 50 IU BTA ( n = 5) towards the SPG in 10 patients with intractable chronic cluster headache with a follow-up of 24 weeks. The primary outcome was adverse events (AEs) and the main efficacy outcome was attack frequency in weeks 3 and 4 post-treatment. Results A total of 11 AEs were registered. There was one severe adverse event (SAE): posterior epistaxis. The number of cluster headache attacks (main efficacy outcome) was statistically significantly reduced in the intention-to-treat analysis from 18 ± 12 per week in baseline to 11 ± 14 ( p = 0.038) in weeks 3 and 4, and five out of 10 patients had at least 50% reduction of attack frequency compared to baseline. The cluster attack frequency was significantly reduced for five out of six months post-treatment. Conclusion Randomised, placebo-controlled studies are warranted to establish the potential of this possible novel treatment of cluster headache.
Visual Priming and Visual Hallucinations in Parkinson’s Disease. Evidence for Normal Top-Down Processes
Authors: Straughan Sarah , Collerton Daniel , Bruce Vicki 
Published: August 2015

Background: Visual hallucinations (VH) are common in Parkinson’s disease (PD) and are associated with increased morbidity and mortality. Current explanations for VH in PD suggest combined impairments in top-down attentional and bottom-up perceptual processes, which allow the passive “release” of stored images. Alternative models in other disorders have suggested that top-down factors may actively encourage hallucinations. In order to explore the interaction between top-down and bottom-up visual processing in PDVH, we developed novel experimental priming tasks in which top-down verbal cues were used to prime the bottom-up recognition of partial or ambiguous pictures. Method: Two groups of PD participants with (PD + VH, n = 16), and without VH (PD - VH, n = 20) were compared to a group of healthy older adults (NC, n = 20) on 3 novel measures of visual priming. Results: All tasks showed significant priming effects. The PD + VH group was more impaired at accurately identifying silhouette and fragmented images compared to the PD - VH group. There were no differences in priming between the 2 PD groups. Conclusions: The study showed that VH in PD are not associated with relatively greater top-down activation, and that the interaction between top-down and bottom-up processes is intact.
Increased Osseous 99mTc-DPD Uptake in End-Stage Ankle Osteoarthritis
Authors: Paul Jochen , Barg Alexej , Kretzschmar Martin , Pagenstert Geert , Studler Ueli , Hügle Thomas , Wegner Nicholas J. , Valderrabano Victor , Geurts Jeroen 
Published: August 2015

Background: We analyzed the histopathologic findings in end-stage osteoarthritic ankle joint tissue that display increased uptake of bone-seeking radiotracer in single-photon emission computed tomography–computed tomography (SPECT-CT) imaging. Methods: Six consecutive patients with end-stage osteoarthritis undergoing total ankle replacement received preoperative SPECT-CT imaging using 99mTechnetium dicarboxypropane diphosphonate (99mTc-DPD). Using imaging data for stratification, osteochondral tissue sections were prepared from SPECT-positive (+) and -negative (–) areas of tibial and talar resection specimens. Histomorphometric analyses of osteoblast numbers, collagen deposition, and cartilage degeneration were performed on hematoxylin and eosin, van Gieson’s and Safranin- O stained tissue sections. Osteoclast activity was visualized using tartrate-resistant acid phosphatase (TRAP) staining. Results: Increased 99mTc-DPD uptake was observed exclusively subjacent to the subchondral bone plate of tibial and talar joint compartments. SPECT(–) tissues displayed typical fatty marrow morphology containing mainly collagen-positive blood vessels and few marrow and bone-lining cells. SPECT(+) tissues were characterized by increased numbers of active bone-lining osteoblasts depositing collagen fibers. Collagen area fraction of subchondral bone marrow was significantly increased in SPECT(+) (0.52 ± 0.21) compared with SPECT(–) (0.29 ± 0.13) tissues ( P = .30). Multinucleated TRAP(+) osteoclasts were absent from bone formation sites, but associated with vascular structures invading articular cartilage through the subchondral bone plate. Increased 99mTc-DPD uptake was specifically and strongly correlated with increased osteoblast numbers ( P = .011), and with collagen area fraction ( P = .030) but not with Mankin score ( P = .202), or with osteoclast number ( P = .576). Conclusion: Subchondral bone tissues in SPECT(+) areas of end-stage ankle osteoarthritis were histologically characterized by increased osteoblast-mediated bone formation in the absence of functional osteoclasts, and increased cellularity and collagen deposition in marrow tissues. Clinical Significance: Our findings suggest a pathologic bone-remodeling process in end-stage ankle OA areas with increased 99mTc-DPD uptake. Level of Evidence: Level IV, case series.
Randomized controlled equivalence trial comparing videoconference and in person delivery of cognitive processing therapy for PTSD
Authors: Maieritsch Kelly P , Smith Tracey L , Hessinger Jonathan D , Ahearn Eileen P , Eickhoff Jens C , Zhao Qianqian 
Published: August 2015

Introduction In an effort to improve access to and utilization of health care, the Veterans Health Administration (VHA) continues to investigate the effectiveness of video-teleconferencing (VTC) technologies for service delivery. While previous research focused on the efficacy of VTC treatment for post-traumatic stress disorder (PTSD) in Vietnam era veterans, few studies have evaluated the efficacy of this modality and treatment for the Iraq/Afghanistan era veterans. The aim of this randomized clinical trial was to evaluate equivalence between in person and VTC psychotherapy for PTSD in this newer cohort. Methods Veterans of the Iraq/Afghanistan conflict from two VHA hospitals in the United States were recruited and randomized to receive cognitive processing therapy (CPT) for PTSD either in person (IP) or over VTC. Clinician-administered and self-report measures were collected before, during, and after treatment. Results A trend was observed which suggested that CPT over VTC may be equivalent to the treatment delivered in person, as suggested by previous studies. Regardless of treatment, veterans who received the intervention in both conditions reported significant decreases on post-treatment measures. Discussion This study highlighted research and clinical challenges in providing services to the newest veteran generation in general as well as unique challenges with VTC. One complicating factor to the statistical power of this study was a treatment dropout rate twice the original estimate. Factors that could have influenced this high dropout rate are explored.
Microcatheter entrapment retrieval from Onyx embolization in brain arteriovenous malformations: A technical note
Authors: Vu Phat D , Grigorian Arthur A 
Published: August 2015

Objective Many techniques have been use for retrieval of an entrapped microcatheter during Onyx (eV3 Neurovascular) embolization of brain arteriovenous malformations (BAVMs). We report our technique that we term “pull-push-pull” that can be utilized as first management in retrieving the microcatheter. Method We analyzed a total of 37 patients that underwent BAVM embolization with either Onyx 18 or 34 at our institution. Standard embolization techniques were utilized with the use of Marathon (eV3 Neurovascular) microcatheter. When difficulty in retrieving the microcatheter arose, we used the “pull-push-pull” technique. The technique comprises the eV3 protocol of retraction. In addition, the microcatheter is stretched causing the Onyx cast to stretch in its inner core, creating a more thorough cohesive property amongst the Onyx mixture. Then the microcatheter is pushed back and to its point of embolization origin. Afterwards, retraction of the microcatheter is enabled as it can be easily dislodged from the cast. Multiple attempts can be repeated as needed. Result and discussion We had three patients that had difficulty with removal of microcatheter (8.1%). Utilization of the “pull-push-pull” technique was used on two of those patients. No neurological complication was observed with our technique. We believe the cohesive property of Onyx solution helps in the retrieval of the catheter by our method and technique. Conclusion We believe the “pull-push-pull” can be utilized and be an additional technique before attempting other catheter retrieval techniques in Onyx BAVM embolization.
Platelets induce apoptosis via membrane-bound FasL
Authors: Schleicher Rebecca I. , Reichenbach Frank , Kraft Peter , Kumar Anil , Lescan Mario , Todt Franziska , Göbel Kerstin , Hilgendorf Ingo , Geisler Tobias , Bauer Axel , Olbrich Marcus , Schaller Martin , Wesselborg Sebastian , O’Reilly Lorraine , Meuth Sven G. , Schulze-Osthoff Klaus , Gawaz Meinrad , Li Xuri , Kleinschnitz Christoph , Edlich Frank , Langer Harald F. 
Published: August 2015

Key PointsPLTs contribute to apoptosis in vivo and express the death receptor ligand FasL upon activation. Membrane-bound FasL mediates PLT-induced apoptosis, whereas Bax/Bak signaling is not required but reinforces PLT-induced apoptosis.
Topical resiquimod can induce disease regression and enhance T-cell effector functions in cutaneous T-cell lymphoma
Authors: Rook Alain H. , Gelfand Joel M. , Wysocka Maria , Troxel Andrea B. , Benoit Bernice , Surber Christian , Elenitsas Rosalie , Buchanan Marie A. , Leahy Deborah S. , Watanabe Rei , Kirsch Ilan R. , Kim Ellen J. , Clark Rachael A. 
Published: July 2015

Key PointsTopical resiquimod is a safe, effective therapy for early-stage CTCL that can clear both treated and untreated skin lesions. Responding patients had T-cell recruitment into skin, enhanced T-cell effector functions, and eradication of the malignant T-cell clones.
Interpretation for practice guidelines for prevention, diagnosis, and treatment of ventilator-associated pneumonia in burn patients by american burn association
Authors: Luo Jie , Guo Guang-hua 
Published: July 2015

Abstract
“American Burn Association Practice Guidelines for Prevention, Diagnosis, and Treatment of Ventilator-Associated Pneumonia in Burn Patients” was published to provide recommendation for the prevention, diagnosis, and treatment of ventilator-associated pneumonia in burn patients. This article makes interpretations and conclusions for prevention, diagnosis and treatment from this guideline in the combination of domestic burn patients.