bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of polyamine levels on the degradation of short-lived and long-lived proteins in cultured L-132 human lung cells
Authors: CORELLA Dolores , GUILLÉN Marisa , HERNÁNDEZ José Miguel , HERNÁNDEZ-YAGO José 
Published: August 2015

Biogenic polyamines have important regulatory functions in various biological processes and it has also been suggested that they could modulate intracellular protein degradation. For an overall assessment of the role of polyamines in this process, we have investigated the effect that the decrease in intracellular polyamine levels caused by inhibitors of polyamine biosynthesis brings about on the degradation of the pools of short- and long-lived proteins in cultured L-132 human lung cells. Treatment of cells with 100 µM (2R,5R)-δ-methyl acetylenic putrescine (MAP), a potent enzyme-activated irreversible inhibitor of ornithine decarboxylase, or with 100 µM MAP plus 50 µM N-butyl 1,3-diaminopropane, a specific inhibitor of spermine synthase, caused a similar decrease (65–70% of control) in the total intracellular levels of polyamines, although they affected the concentrations of spermidine and spermine differently. The effect of the two treatments on protein degradation was essentially the same. In polyamine-depleted cells we observed an inhibition of degradation in long-lived proteins of 16% (P&lt; 0.05), with a significant increase in the half-life (t½) of this pool from 100.5 to 120.1 h. This was concomitant with an increase of 26% (P&lt; 0.05) in degradation in short-lived proteins, with a significant decrease in the t½ of this pool from 0.85 to 0.67 h. Recovery of polyamine levels by the addition of 50 µM spermidine to polyamine-depleted cells resulted in a restoration of the degradation rates in both pools of proteins. The way(s) by which polyamines could modulate proteolysis are discussed.
Transcription of the juvenile hormone esterase gene under the control of both an initiator and AT-rich motif
Authors: JONES Grace , MANCZAK Maria , SCHELLING Douglas , TURNER Helen , JONES Davy 
Published: August 2015

The binding of transcription factors to the core promoter of the juvenile hormone esterase gene was functionally characterized using both a cell-free in vitrotranscription functional assay and a cell transfection assay. A core JHE promoter (-61 to +28 bp relative to transcription start site) supported faithful transcription from the in vivo transcription start site. The nuclear extracts from the Sf9 insect cell line that provided transcription from that template also bound to that template as a probe in gel-mobility shift assays. Deletion or transversion of the initiator-binding motif (-1 to +4 bp) abolished detectable transcription either in vitro or in transfected cells. An AT-rich motif (ATATAT; -28 to -23 bp) serves another transcription factor-binding site. Mutation of the AT-rich motif to a canonical TATA-box preserved transcription, while either its deletion or complete transversion abolished or significantly reduced detectable transcriptional activity. These results indicate that, under these conditions, the functional operation of this core promoter approaches that of a composite promoter in which both the TATA- and initiator-binding protein complexes are necessary, even for basal transcription. On the other hand, these debilitating mutations to either the TATA box or initiator motif did not prevent the ability of the corresponding gel-shift competitive probes to compete with the wild-type promoter for binding by the transcription factors. Even a double transversion of both the AT-rich motif and the initiator-binding motif was able to competitively displace the protein complex that bound to the labelled wild-type probe. These data strongly indicate the presence of (an) additional core-promoter-associated transcription factor(s) (that is not the ‘downstream element ’) that contact(s) the AT-binding complex and/or initiator-binding factor with sufficient avidity to remove them from binding to the competing wild-type promoter sequence.
Two HNF-1 binding sites govern the glucose repression of the human sucrase-isomaltase promoter
Authors: RODOLOSSE Annie , CARRIERE Véronique , ROUSSET Monique , LACASA Michel 
Published: August 2015

We have previously shown, using the Caco-2 clone PF11, that glucose represses transcription of the human sucrase-isomaltase (SI) gene and that the -370/+30 fragment of the SI gene conferred glucose-regulated expression on a heterologous gene. Different fragments beginning at the already characterized SI footprint (SIF) 1 (-53/-37), SIFR (-153/-129) or SIF3 (-176/-156) elements [Wu, Chen, Forslund and Traber (1994) J. Biol. Chem. 269, 17080–17085] were tested, in comparison with the -370/+30 fragment, for their capacity to inhibit reporter gene expression under high-glucose (25 mM) conditions. Unlike SIF1 and SIFR, the addition of the HNF (hepatocyte nuclear factor)-1-binding element SIF3 to the promoter fragment was required for repression under high-glucose conditions. This effect was enhanced when the SI promoter was extended to position -370, indicating that the -370/-176 region contains elements that may co-operate with SIF3 to increase the metabolic control of the SI promoter. We have characterized an additional HNF-1-binding site near to and upstream from SIF3; SIF4. By mutagenesis of the three HNF-1-binding elements we show that the two distal HNF-1-recognition sites are the most important for the glucose regulation of the SI gene. Moreover, this glucose regulation was abolished in PF11 cells overexpressing vHNF-1C (variant HNF, an isoform of the HNF-1 family). We thus propose that the differential binding of HNF-1-family proteins to their DNA targets on the SI promoter constitutes the molecular mechanism that controls the glucose regulation of the SI gene transcription.
Cloning of an intracellular protein that binds selectively to mitogenic acidic fibroblast growth factor
Authors: KOLPAKOVA Elona , WIĘDŁOCHA Antoni , STENMARK Harald , KLINGENBERG Olav , FALNES Pål Ø. , OLSNES Sjur 
Published: August 2015

In addition to its extracellular action, there is evidence that acidic fibroblast growth factor (aFGF) acts inside cells. To identify intracellular proteins interacting with aFGF, we screened a HeLa cell library in the yeast two-hybrid system using pLex-aFGF as a bait. A clone binding to aFGF, but not to the non-mitogenic mutant aFGF-K132E, was isolated and characterized. The insert contained an open reading frame corresponding to a novel protein of 42 kDa. The protein, termed aFGF intracellular binding protein (FIBP), is mainly hydrophilic and does not contain an N-terminal signal sequence. In vitro-translated FIBP bound specifically to a fusion protein of maltose-binding protein and aFGF. FIBP became post-translationally associated with microsomes added to the cell-free protein synthesizing system, and the membrane-associated protein bound aFGF with high efficiency. Immunoblots and fluorescence microscopy demonstrated that the protein is present in nuclei and, to a lesser extent, associated with mitochondria and other cytoplasmic membranes. The possibility is discussed that FIBP may be involved in the mitogenic action of aFGF.
The nucleotide sequences described in this paper have been submitted to GeneBank database under accession numbers AF010187 (human FIBP) and AF010188 (simian FIBP).
Effects of competition on the sleep patterns of elite rugby union players
Authors: Shearer David A. , Jones Rhys M. , Kilduff Liam P. , Cook Christian J. 
Published: August 2015

AbstractNo published research has assessed sleep patterns of elite rugby union players following match‐play. The present study examined sleep patterns of professional rugby union players, prior and post‐match‐play, to assess the influence of competition. Twenty‐eight male rugby union players (24.4 ± 2.9 years, 103.9 ± 12.2 kg) competed in one of four competitive home matches. Player's sleep behaviours were monitored continuously using an Actiwatch® from two days before the match, until three days post‐match. Repeated measures of analysis of variance (ANOVA) showed significant differences across the time points measured for time to bed (F = 26.425, η2
= 0.495, p <. 001), get up time (F = 21.175, η2 = 0.440, p < .001), time spent in bed (F = 10.669, η2 = 0.283, p < .001), time asleep (F = 8.752, η2 = 0.245, p < .001) and percentage of time moving (F = 4.602, η2 = 0.146 p < .05). Most notable, post hocs revealed a significant increase for time in bed the night before the match (p < .01; 95% CI = 0 : 10–1 : 28 h; 9.7 ± 13.5%) compared with the reference night sleep. Furthermore, time asleep significantly decreased post‐match (p < .05; 95% CI = −0:03 to −1:59 h; −19.5 ± 19.8%) compared to two nights pre‐match. Across all time points, sleep latency and efficiency for most players were considered abnormal compared to that expected in normal populations. The results demonstrate that sleep that is deprived post‐match may have detrimental effects on the recovery process.
Recovery Rates of Human Fetal Skeletal Remains Using Varying Mesh Sizes
Authors: Pokines James T. , De La Paz Jade S. 
Published: August 2015

AbstractHuman fetal skeletal elements of different gestational ages were screened with multiple mesh sizes (6.4 mm [1/4 inch], 3.2 mm [1/8 inch], 2.0 mm, and 1.0 mm) to determine their recovery rates. All remains were previously macerated, and no significantly damaged elements were used. The 6.4 mm mesh allowed a large loss of elements (63.2% overall), including diagnostic elements, while no diagnostic elements were lost when the 1 mm mesh (0.2%) was used. When using the 3.2 mm mesh, 16.2% of the bones were lost, including some diagnostic elements (primarily tooth crowns), while 7.5% were lost using the 2.0 mm mesh. The authors recommend that the potential loss of information incurred when utilizing larger mesh sizes be taken into consideration when planning recovery methods where fetal remains may be encountered and that a minimum of 1.0 mm mesh be utilized in recovery contexts known to include fetal remains.
LeSSS: Learned Shared Semantic Spaces for Relating Multi‐Modal Representations of 3D Shapes
Authors: Herzog Robert , Mewes Daniel , Wand Michael , Guibas Leonidas , Seidel Hans‐Peter 
Published: August 2015

AbstractIn this paper, we propose a new method for structuring multi‐modal representations of shapes according to semantic relations. We learn a metric that links semantically similar objects represented in different modalities. First, 3D‐shapes are associated with textual labels by learning how textual attributes are related to the observed geometry. Correlations between similar labels are captured by simultaneously embedding labels and shape descriptors into a common latent space in which an inner product corresponds to similarity. The mapping is learned robustly by optimizing a rank‐based loss function under a sparseness prior for the spectrum of the matrix of all classifiers. Second, we extend this framework towards relating multi‐modal representations of the geometric objects. The key idea is that weak cues from shared human labels are sufficient to obtain a consistent embedding of related objects even though their representations are not directly comparable. We evaluate our method against common base‐line approaches, investigate the influence of different geometric descriptors, and demonstrate a prototypical multi‐modal browser that relates 3D‐objects with text, photographs, and 2D line sketches.
Adenosine monophosphate is not superior to histamine for bronchial provocation test for assessment of asthma control and symptoms
Authors: Wu Fan , Guan Wei‐Jie , Gao Yi , An Jia‐Ying , Xie Yan‐Qing , Liu Wen‐Ting , Yu Xin‐Xin , Zheng Jin‐Ping 
Published: August 2015

AbstractBackgroundAdenosine monophosphate (AMP) may reflect airway inflammation and hyperresponsiveness, but relationship between AMP and histamine (His, a conventional stimulus) bronchial provocation test (BPT) in asthma is not fully elucidated.ObjectivesTo compare both BPTs and determine their utility in reflecting changes of asthmatic symptoms.MethodsBPTs were performed in a cross‐over fashion, at 2–4 day intervals. Cumulative doses eliciting 20% FEV1fall (PD20FEV1), diagnostic performance and adverse events (AEs) were compared. Patients with PD20FEV1 lower than geometric mean were defined as responders, otherwise poor responders. Patients with uncontrolled and partly controlled asthma, who maintained their original inhaled corticosteroids therapy, underwent reassessment of airway responsiveness and asthmatic symptoms 3 and 6 months after.ResultsNineteen uncontrolled, 22 partly controlled and 19 controlled asthmatic patients and 24 healthy subjects were recruited. Lower PD20FEV1 geometric means were associated with poorer asthma control in His‐BPT (0.424 μmol vs 1.684 μmol vs 3.757 μmol), but not AMP‐BPT (11.810 μmol vs 7.781 μmol vs 10.220 μmol). Both BPTs yielded similar overall diagnostic performance in asthma (area under curve: 0.842 in AMP‐BPT vs 0.850 in His‐BPT). AEs, including wheezing and tachypnea, were similar and mild. Ten patients with uncontrolled and 10 partly controlled asthma were followed‐up. At months 3 and 6, we documented an increase in PD20FEV1‐AMP and PD20FEV1‐His, which did not correlate with reduction asthmatic symptom scores. This overall applied in responders and poor responders of AMP‐BPT and His‐BPT.ConclusionDespite higher screening capacity of well‐controlled asthma, AMP‐BPT confers similar diagnostic performance and safety with His‐BPT. AMP‐BPT might not preferentially reflect changes asthmatic symptoms.
Adaptive Tactical Pricing in Multi‐Agent Supply Chain Markets Using Economic Regimes
Authors: Hogenboom Alexander , Ketter Wolfgang , van Dalen Jan , Kaymak Uzay , Collins John , Gupta Alok 
Published: August 2015

ABSTRACTIn today's complex and dynamic supply chain markets, information systems are essential for effective supply chain management. Complex decision making processes on strategic, tactical, and operational levels require substantial timely support in order to contribute to organizations' agility. Consequently, there is a need for sophisticated dynamic product pricing mechanisms that can adapt quickly to changing market conditions and competitors' strategies. We propose a two‐layered machine learning approach to compute tactical pricing decisions in real time. The first layer estimates prevailing economic conditions—economic regimes—identifying and predicting current and future market conditions. In the second layer, we train a neural network for each regime to estimate price distributions in real time using available information. The neural networks compute offer acceptance probabilities from a tactical perspective to meet desired sales quotas. We validate our approach in the trading agent competition for supply chain management. When competing against the world's leading agents, the performance of our system significantly improves compared to using only economic regimes to predict prices. Profits increase significantly even though the prices and sales volume do not change significantly. Instead, tactical pricing results in a more efficient sales strategy by reducing both finished goods and components inventory costs.
A global, remote sensing‐based characterization of terrestrial habitat heterogeneity for biodiversity and ecosystem modelling
Authors: Tuanmu Mao‐Ning , Jetz Walter 
Published: August 2015

AbstractAimHabitat heterogeneity has long been recognized as a key landscape characteristic determining biodiversity patterns. However, a lack of standardized, large‐scale, high‐resolution and temporally updatable heterogeneity information based on direct observations has limited our understanding of this connection and its effective use for biodiversity conservation. To address this, we develop here remote sensing‐based metrics to characterize global habitat heterogeneity at 1‐km resolution and assess their value for biodiversity modelling.LocationGlobal.MethodsWe develop 14 heterogeneity metrics (available athttp://www.earthenv.org) based on the textural features of the enhanced vegetation index (EVI) imagery from the Moderate Resolution Imaging Spectroradiometer (MODIS), and closely examine a complementary core set of six of these metrics. We evaluate their ability to provide fine‐grain habitat heterogeneity by comparing the heterogeneity information captured by them with that measured by 30‐m Landsat‐based land‐cover data. Using spatial autoregressive models, we then compare their utility with that of more conventional metrics (derived from topography or categorical land‐cover data) for modelling the species richness of bird communities across the conterminousUnitedStates based onBreedingBirdSurvey data.ResultsThe newly derived metrics capture different aspects of habitat heterogeneity and provide fine‐grain information for locations deemed homogeneous by traditional land‐cover classifications at both continental and global extents. Most of them strongly exceed conventional heterogeneity variables in capturing the spatial variation in bird species richness, withHomogeneity emerging as the strongest predictor.Main conclusionsThis study develops and validates the performance of readily usable metrics of textural measures capturing fine‐grain habitat heterogeneity. The presented metrics outperform conventional measures in capturing detailed spatial variation in habitats and in predicting key biodiversity patterns. They provide a rigorous and comparable basis for understanding heterogeneity–diversity relationships, and offer a powerful tool for monitoring and understanding the responses of biodiversity and ecosystems to the changing environment.
Analysis of limb segments length and body proportion of southern Chinese children and adolescents
Authors: Zhu Min , Jiao Yan‐Hua , Xiong Feng , Xie Fang , Guo Shu‐Juan , Cun Yue‐Shuang 
Published: August 2015

AimThis cross‐sectional study was designed to characterize by age the ratios of limb segments length to height and extremities–trunk ratio and body proportions of southern Chinese children.MethodsData were collected from students (n = 4715) from five school, aged 6–17 years, in the city of Chongqing. Their standing height, sitting height, arm span, forearm length, upper arm length, leg length, lower leg length, and ratios of extremities to trunk length were determined.ResultsSitting height, forearm length, upper arm length, arm span, and lower leg length were highly correlated with standing height (r > 0.9; P < 0.05). The ratio of extremities to trunk increased till about 13 years of age for both genders.ConclusionsThe length of extremities and their ratio to sitting height reflect regular changes of growth in Chinese children as their age, and limb segments length is highly correlated with height.
Exposure assessment of epidermal growth factor to various tissues in mice after intravenous and subcutaneous administration
Authors: Lee Jong Bong , Shin Beomsoo , Lee Sang Ho , Lee Bong Yong , Kim Tae Hwan , Kim Min Gi , Yoo Sun Dong 
Published: August 2015

Abstract

Objectives
This study was conducted to examine the tissue distribution of human recombinant epidermal growth factor (EGF) after multiple intravenous and subcutaneous injections in mice.

Methods
Male BALB/c mice were divided into (1) EGF 1 mg/kg intravenous dose, (2) EGF 5 mg/kg intravenous dose, (3) drug-free intravenous control, (4) EGF 1 mg/kg subcutaneous dose, (5) EGF 5 mg/kg subcutaneous dose and (6) drug-free subcutaneous control groups. EGF and drug-free dosing solutions were injected by intravenous and subcutaneous injections once a day for 3 days. EGF concentrations in serum and tissues of kidney, liver, lung, small intestine and tongue were determined by ELISA.

Key findings
As the intravenous and subcutaneous doses were increased from 1 to 5 mg/kg, serum Cmax and area under the concentration–time curve (AUC) values were increased dose-proportionally. In lung, tongue and small intestine, increases in AUC were dose-proportional after intravenous injections, but greater than dose-proportional after subcutaneous injections. The fold-increases in Cmax and AUC values were lowest in liver and highest in kidney.

Conclusion
Based on Cmax and AUC data, the systemic exposure achieved by subcutaneous injections was comparable with that achieved by intravenous injections.
Distinct effects of novel naphtoquinone-based triazoles in human leukaemic cell lines
Authors: Coulidiati Tangbadioa H. , Dantas Bruna B. , Faheina-Martins Glaucia V. , Gonçalves Juan C. R. , do Nascimento Wilson S. , de Oliveira Ronaldo N. , Camara Celso A. , Oliveira Eduardo J. , Lara Aline , Gomes Eneas R. , Araújo Demetrius A. M. 
Published: August 2015

Abstract

Objectives
The aim of this study was to investigate the cytotoxic effect of new 1,4-naphthoquinone- 1,2,3-triazoles, named C2 to C8 triazole derivatives, towards human cancer cell lines.

Methods
The effect on cell viability was assessed by MTT and propidium iodide assays. The cytotoxic effect of C2 and C3 in K562 and HL-60 cells were analyzed by flow cytometry, DNA fragmentation and reactive oxygen species (ROS) production. Western blot and q-PCR procedures were also performed.

Key findings
C2 and C3 inhibited both K562 and HL-60 cells growth in a concentration-dependent manner. C2 presented the highest cytotoxic activity with an IC50 of approximately 14 μm and 41 μm for HL-60 and K562 cells, respectively, while being less toxic to normal peripheral blood monocyte cells. Both derivatives induced cellular changes in HL-60 cells, characteristic of apoptosis, such as mitochondrial membrane depolarization, phosphatidylserine externalization, increasing sub-G1 phase, DNA fragmentation, downregulating Bcl-2 protein and upregulating Bax protein. In K562 cells, C2 and C3 induced S-phase arrest of cell cycle, which was associated with upregulation of p21. The effect of these derivatives in HL-60 cells can be related to the ROS intracellular level.

Conclusion
Taken together our results showed that C2 and C3 triazole derivatives presented the best potential for drug design.
Heart-Rate Changes After an Ultraendurance Swim From Italy to Albania: A Case Report
Authors: Valenzano Anna , Moscatelli Fiorenzo , Triggiani Antonio Ivano , Capranica Laura , De Ioannon Giulia , Piacentini Maria Francesca , Mignardi Sergio , Messina Giovanni , Villani Stefano , Cibelli Giuseppe 
Published: August 2015

Purpose:To evaluate the effect of a solo ultraendurance open-water swim on autonomic and nonautonomic control of heart rate (HR).Methods:A male athlete (age 48 y, height 172 cm, body mass 68 kg, BMI 23 kg/m2) underwent HR-variability (HRV) and circulating catecholamine evaluations at different times before and after an ultraendurance swim crossing the Adriatic Sea from Italy to Albania. HRV was measured in 5-min segments and quantified by time and frequency domain. Circulating catecholamines were estimated by salivary alpha-amylase (sAA) assay.Results:The athlete completed 78.1 km in 23:44 h:min. After arrival, sAA levels had increased by 102.6%. Time- and frequency-domain HRV indexes decreased, as well (mean RR interval, −29,7%; standard deviation of normal mean RR interval, −63,1%; square root of mean squared successive differences between normal-to-normal RR intervals, −49.3%; total power, −74.3%; low frequency, −78.0%; high frequency, −76.4%), while HR increased by 41.8%. At 16-h recovery, sAA had returned to preevent values, while a stable tachycardia was accompanied by reduced HRV measures.Conclusion:To the authors’ knowledge, this is the first study reporting cardiac autonomic adjustments to an extreme and challenging ultraendurance open-water swim. The findings confirmed that the autonomic drives depend on exercise efforts. Since HRV changes did not mirror the catecholamine response 16 h postevent, the authors assume that the ultraendurance swim differently influenced cardiac function by both adaptive autonomic and nonautonomic patterns.