bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glucose-lactate metabolic cooperation in cancer: insights from a spatial mathematical model and implications for targeted therapy
Authors: McGillen Jessica B. , Kelly Catherine J. , Martíez-González Alicia , Martin Natasha K. , Gaffney Eamonn A. , Maini Philip K. , Pérez-García Vıctor M. 
Published: September 2014

AbstractA recent hypothesis has proposed a glucose-lactate metabolic symbiosis between adjacent hypoxic and oxygenated regions of a developing tumour, and proposed a treatment strategy to target this symbiosis. However,in vivoexperimental support remains inconclusive. Here we develop a minimal spatial mathematical model of glucose-lactate metabolism to examine, in principle, whether metabolic symbiosis is plausible in human tumours, and to assess the potential impact of inhibiting it. We find that symbiosis is a robust feature of our model system—although on the length scale at which oxygen supply is diffusion-limited, its occurrence requires very high cellular metabolic activity—and that necrosis in the tumour core is reduced in the presence of symbiosis. Upon simulating therapeutic inhibition of lactate uptake, we predict that targeted treatment increases the extent of tissue oxygenation without increasing core necrosis. The oxygenation effect is correlated strongly with the extent of wildtype hypoxia and only weakly with wildtype symbiotic behaviour, and therefore may be promising for radiosensitisation of hypoxic, lactate-consuming tumours even if they do not exhibit a spatially well-defined symbiosis. Finally, we conduct a set ofin vitroexperiments on the U87 glioblastoma cell line to facilitate preliminary speculation as to where highly malignant tumours might fall in our parameter space, and find that these experiments suggest a weakly symbiotic regime for U87 cells, which raises the new question of what relationship exists between symbiosis—if indeed it occursin vivo—and tumour malignancy.
Crystal structure of (pyridin-2-ylmethylidene)(triphenylmethyl)amine
Authors: Theppitak Chatphorn , Meesangkaew Manlika , Chanthee Songwuit , Sriprang Nimit , Chainok Kittipong 
Published: September 2014

The title Schiff base compound, C25H20N2, crystallizes with two independent molecules (AandB) in the asymmetric unit. In both molecules, the imine group is approximately coplanar with the pyridine ring, with N—C—C—N torsion angles of 170.1 (3) and −172.0 (3) Å. In the crystal,A and Bdimers are linked by pairs of C—H...π interactions and further C—H...π bonds link the dimers into a three-dimensional network.
Roles of trust on rapport and satisfaction in services
Authors: Kayeser Fatima Johra , Abdur Razzaque Mohammed 
Published: September 2014

Purpose
– The purpose of this paper is to report antecedent roles of competence, contractual and goodwill trust on rapport and satisfaction in banking services in a developing country context. The study also identifies the mediating influence of these three types of trust between rapport and customer satisfaction.

Design/methodology/approach
– Data collected from a sample of 212 bank customers were analysed using AMOS-based structural equation modelling.

Findings
– While all three types of trust have significant impact on satisfaction, only competence and goodwill trust positively influenced rapport. Results also demonstrate that competence and goodwill trust had complementary mediation impacts in the rapport-satisfaction context; however, mediation influence of contractual trust was not significant. Finally, influence of rapport was found to be positive towards satisfaction.

Practical implications
– Management should focus on building goodwill and competence trust with bank customers. Bank managers should hire and train employees who are friendly in terms of customer care and are able to develop rapport with customers to increase the latter's satisfaction.

Originality/value
– The paper tested the influence of competence, contractual and goodwill trust on rapport and satisfaction and verified the mediation impact of trust between rapport and satisfaction.
The effects of customer satisfaction, perceived value, corporate image and service quality on behavioral intentions in gaming establishments
Authors: Wu Hung-Che 
Published: September 2014

Purpose– The purpose of this paper is to identify the dimensions of service quality and examining the interrelationships among behavioral intentions, customer satisfaction, perceived value, corporate image and service quality in the gaming industry. A multi-level and hierarchical model is used as a framework to synthesize the effects of customer satisfaction, perceived value, corporate image and service quality on behavioral intentions of customers in the gaming industry.Design/methodology/approach– The data used in this study were based on a sample of 470 at a newly built casino in Macau. Data were analyzed using exploratory factor analysis and regression analysis.Findings– The findings support using a multi-level model consisting of three primary dimensions and ten sub-dimensions to conceptualize and measure perceived service quality. Perceived service quality significantly influences perceived value and corporate image, respectively. In addition, perceived value and corporate image are main determinants of customer satisfaction. Also, customer satisfaction and corporate image significantly affect behavioral intentions.Originality/value– This is the first study synthesizing behavioral intentions, customer satisfaction, perceived value, corporate image and perceived service quality in a Macau casino setting.
Interactive effects of C, N and P fertilization on soil microbial community structure and function in an Amazonian rain forest
Authors: Fanin Nicolas , Hättenschwiler Stephan , Schimann Heidy , Fromin Nathalie 
Published: September 2014

Summary

Resource control over abundance, structure and functional diversity of soil microbial communities is a key determinant of soil processes and related ecosystem functioning. Copiotrophic organisms tend to be found in environments which are rich in nutrients, particularly carbon, in contrast to oligotrophs, which survive in much lower carbon concentrations.

We hypothesized that microbial biomass, activity and community structure in nutrient‐poor soils of an Amazonian rain forest are limited by multiple elements in interaction. We tested this hypothesis with a fertilization experiment by adding C (as cellulose), N (as urea) and P (as phosphate) in all possible combinations to a total of 40 plots of an undisturbed tropical forest in French Guiana.

After 2 years of fertilization, we measured a 47% higher biomass, a 21% increase in substrate‐induced respiration rate and a 5‐fold higher rate of decomposition of cellulose paper discs of soil microbial communities that grew in P‐fertilized plots compared to plots without P fertilization. These responses were amplified with a simultaneous C fertilization suggesting P and C colimitation of soil micro‐organisms at our study site.

Moreover, P fertilization modified microbial community structure (PLFAs) to a more copiotrophic bacterial community indicated by a significant decrease in the Gram‐positive : Gram‐negative ratio. The Fungi : Bacteria ratio increased in N fertilized plots, suggesting that fungi are relatively more limited by N than bacteria. Changes in microbial community structure did not affect rates of general processes such as glucose mineralization and cellulose paper decomposition. In contrast, community level physiological profiles under P fertilization combined with either C or N fertilization or both differed strongly from all other treatments, indicating functionally different microbial communities.

While P appears to be the most critical from the three major elements we manipulated, the strongest effects were observed in combination with either supplementary C or N addition in support of multiple element control on soil microbial functioning and community structure.

We conclude that the soil microbial community in the studied tropical rain forest and the processes it drives is finely tuned by the relative availability in C, N and P. Any shifts in the relative abundance of these key elements may affect spatial and temporal heterogeneity in microbial community structure, their associated functions and the dynamics of C and nutrients in tropical ecosystems.
Cardiac Injury Is a Common Postmortem Finding in Thrombotic Thrombocytopenic Purpura Patients: Is Empiric Cardiac Monitoring and Protection Needed?
Authors: Nichols Larry , Berg Aaron , Rollins‐Raval Marian A , Raval Jay S 
Published: September 2014

AbstractThrombotic thrombocytopenic purpura (TTP) is a rare and potentially fatal disease. Early implementation of therapeutic plasma exchange (TPE) has decreased the mortality rate from >90% to <10%. However, fatalities still occur in these patients. The goal of this study was to characterize the causes of death and related postmortem findings in patients with TTP in the current era of emergent TPE to identify possible areas for improvement in the care of these patients. We analyzed clinical history, laboratory and histopathologic findings, and causes of death of patients with active TTP or TTP in clinical remission autopsied at our institution over 22 years. Of 18 patients, 15 had TTP judged to be a cause of death: it was an underlying cause of death in five cases, intermediate in three, and contributing in seven. The most common immediate causes of death were cardiac arrest and myocardial infarction. The most common TTP‐related findings at autopsy were thrombi/emboli in heart (9), lung (11), brain (3), kidney (7), followed by hemorrhages in heart (7), lung (8), brain (2), kidney (7), and infarcts in heart (5), lung (4), brain (6) and kidney (3). Analysis of the cases with TTP as a cause of death suggests that the mechanism of death is commonly cardiac in origin. Proactive measures to monitor and protect the heart may be beneficial in these patients.
Regional Citrate Anticoagulation for Continuous Renal Replacement Therapy in the Perioperative Care of Liver Transplant Recipients: A Single Center Experience
Authors: Sponholz Christoph , Settmacher Utz , Bauer Michael , Kortgen Andreas 
Published: September 2014

AbstractKidney injury with concomitant hemodialysis is a common finding in perioperative care of liver transplant patients. The aim of this study was to evaluate disturbances in acid‐base status, electrolyte balance and citrate accumulation during hemodialysis with regional citrate anticoagulation in perioperative care of liver transplant recipients. A retrospective, single center evaluation was conducted of patients with severe liver dysfunction receiving renal replacement therapy in the perioperative care of liver transplantation in a multidisciplinary ICU of a university hospital. Within 5 days of ICU stay, 89 patients undergoing liver transplantation received regional citrate anticoagulation for hemodialysis. During the study period pH (7.39 [7.33/7.43] vs. 7.44 [7.39/7.47], P‐value = 0.014), base excess values (−0.9 [−5.08/2.35] vs. 4.3 [1.93/8.21], P‐value = 0.001) and standard bicarbonate (23.6 [20/26.9] vs. 28.2 [26.2/32.2], P‐value = 0.001) significantly increased, whereas lactate levels (2.6 [1.60/4.45] vs. 1.25 [0.98/1.9], P‐value = 0.071) and Catot/Caion‐ratio decreased or remained below the upper reference. Hypocalcemia appeared mostly within 48 h after dialysis initiation. Although sodium levels increased during the observation, rates of hypernatremia were comparable between hemodialysis days 1 and 5. Hemodialysis using regional citrate anticoagulation remains a challenge in the perioperative care of liver transplant recipients. Major attention must be paid to acid‐base disturbances and citrate accumulation within 48 h after dialysis initiation. Nevertheless, regional citrate anticoagulation in liver dysfunction is a feasible and valuable tool, when limitations and pitfalls are adequately considered.
Randomized, Double‐Blind, Placebo‐Controlled Trial of Spironolactone for Hypokalemia in Continuous Ambulatory Peritoneal Dialysis Patients
Authors: Yongsiri Somchai , Thammakumpee Jiranuch , Prongnamchai Suriya , Tengpraettanakorn Pechngam , Chueansuwan Rachaneeporn , Tangjaturonrasme Siriporn , Dinchuthai Pakaphan 
Published: September 2014

AbstractThe incidence of hypokalemia in continuous ambulatory peritoneal dialysis (CAPD) patients is about 15–60%, leading to significant complications. There is no standard treatment other than potassium supplement in this setting. The aim of this study was to evaluate effect of spironolactone 25 mg/day in CAPD patients who have a history of hypokalemia. This is a randomized, double‐blind, placebo‐controlled, cross‐over study in CAPD patients who had a history of hypokalemia. Study intervention is 4 weeks of oral spironolactone 25 mg/day or placebo, cross‐over after a 2‐week wash‐out period. The primary outcome was the difference of serum potassium before and after 4 weeks of spironolactone treatment. Serum potassium was measured every 2 weeks, serum magnesium, urine and peritoneal fluid potassium measured before and after each treatment period. We enrolled 24 patients, and 20 completed the cross‐over study. Ten patients were anuric. The total doses of potassium supplement were the same during the study period. Serum potassium levels before and after study intervention were not significantly different in both groups (4.23 ± 0.64 vs. 3.90 ± 0.59 mEq/L for spironolactone P = 0.077 and 3.84 ± 0.62 vs. 3.91 ± 0.52 for placebo P = 0.551). Total 24‐h potassium, magnesium, sodium excretion, urine volume and ultrafiltration volume were not affected by spironolactone or placebo. There was one episode of hyperkalemia (5.6 mEq/L) during the spironolactone treatment period. Spironolactone 25 mg/day does not have a significant effect on serum potassium or urine and peritoneal excretion rate in CAPD patients who have a history of hypokalemia.
Fibroblast Growth Factor 23, but not Parathyroid Hormone, Is Associated With Urinary Phosphate Regulation in Patients on Peritoneal Dialysis
Authors: Yamada Shunsuke , Tsuruya Kazuhiko , Tokumoto Masanori , Yoshida Hisako , Hasegawa Shoko , Tanaka Shigeru , Eriguchi Masahiro , Nakano Toshiaki , Masutani Kosuke , Ooboshi Hiroaki , Kitazono Takanari 
Published: September 2014

AbstractFibroblast growth factor (FGF) 23 plays an important role in regulation of renal phosphate excretion in patients with chronic kidney disease. However, it remains undetermined whether FGF23 is closely linked to renal phosphate handling in patients with low glomerular filtration rate (GFR). The present cross‐sectional study included 52 outpatients undergoing peritoneal dialysis with urine volume ≥ 100 mL/day. The primary outcome was level of urinary phosphate excretion, and the secondary outcomes were tubular maximal reabsorption of phosphate normalized to GFR (TmP/GFR), an index of the renal threshold for phosphate excretion, and level of peritoneal phosphate excretion. Variates of interest were serum FGF23 and parathyroid hormone (PTH) levels. The median and interquartile range of serum FGF23 level, TmP/GFR, and total urinary and peritoneal phosphate excretion were 5610 (1493–11 430) ng/mL, 1.30 (0.44–1.86) mg/dL, 117 (40–234) mg/day, and 208 (156–250) mg/day, respectively. Multivariate linear regression analysis revealed that serum FGF23 level was significantly (P < 0.05) associated with TmP/GFR negatively and significantly (P < 0.05) associated with urinary phosphate excretion positively, even after adjusting for confounders. In contrast, none of the three outcome variates was associated with serum PTH level. Neither serum FGF23 nor PTH level was associated with peritoneal phosphate excretion. The present study indicates that FGF23, but not PTH, is involved in urinary phosphate regulation, even in patients on peritoneal dialysis with residual renal function.
Adherence to insulin treatment in diabetes: can it be improved?糖尿病患者使用胰岛素治疗的依从性：它能够得到改善吗？
Authors: Doggrell Sheila Anne , Chan Vincent 
Published: September 2014

AbstractInsulin is used in all subjects with Type 1 diabetes, and when Type 2 diabetes is not controlled by oral anti‐diabetic medicines, insulin is also used in Type 2 diabetes. However, despite this use, there is still increased mortality and morbidity in subjects with diabetes, compared to subjects without diabetes. One of the factors, which may be involved in this increased mortality and morbidity in subjects with diabetes, is nonadherence to insulin. Nonadherence rates to insulin are in the range of 20–38%, and many factors contribute to the nonadherence. The major aim of the review was to determine whether interventions to improve adherence to insulin do actually improve adherence to insulin. Most studies have shown that adherence to insulin was improved by changing from the vial‐and‐syringe approach to prefilled insulin pens, but not all studies have shown that this translated into better glycemic control and clinical outcomes. The results of studies using automatic telephone messages to improve adherence to insulin to date are inconclusive. There is limited and variable evidence that an intervention by a nurse/educator, which discusses adherence to medicines, does improve adherence to insulin. In contrast, there is little or no evidence that an extra intervention by a doctor or an intervention by a pharmacist, which discusses adherence to insulin, does actually improve the measured adherence to insulin. In conclusion, rather than assuming that an intervention by a health professional discussing adherence to insulin actually improves adherence to insulin, long‐term studies investigating this are required.
Prevalence and characteristics of non‐obese diabetes in Japanese men and women: the Yuport Medical Checkup Center Study 日本男性与女性非肥胖者的糖尿病患病率与特征：Yuport医学体检中心研究
Authors: Kashima Saori , Inoue Kazuo , Matsumoto Masatoshi , Akimoto Kimihiko 
Published: September 2014

AbstractBackgroundThis study examined the prevalence and characteristics of type 2 diabetes in non‐obese subjects to compare their cardiometabolic markers to those without diabetes.MethodsData were used from 17 098 men and 17 199 women from a voluntary health checkup program between 1998 and 2006 conducted in Japan. The prevalence of diabetes (fasting plasma glucose ≥ 7.0 mmol/L, hemoglobin A1c ≥ 6.5%, or known diabetes) was calculated in each subgroup of body mass index (group 1, <22; group 2, 22–25; group3, 25–27.5; and group 4, ≥27.5). The effect of diabetes and obesity on risk of an abnormal level of cardiometabolic marker was evaluated with a logistic regression model.ResultsIn men, the prevalence of diabetes was 9.5% in total, 6.4%, 9.4%, 11.3% and 16.2% in group1 through 4, respectively. In women, it accounted for 4.3% in total, 2.4%, 4.5%, 8.7% and 12.3% per group, respectively. Non‐obese diabetic subjects (in group 1 and 2) accounted for 60.8% and 62.0% of all the diabetic subjects in men and women, respectively. Non‐obese population accounted for 71.2% and 83.6% of all men and women, respectively. Levels of cardiometabolic markers were higher in diabetic subjects than in non‐diabetic subjects in each subgroup. Diabetes was associated with each abnormal level of cardiometabolic marker independent of obesity.ConclusionOver 60% of the diabetic subjects in this Japanese population were not obese. Non‐obese diabetes is not widely addressed and should be considered for increased public attention. The elevated levels of cardiometabolic markers may contribute to an increased risk of cardiovascular disease in non‐obese diabetes.
Methadone is a Non‐Competitive Antagonist at the α4β2 and α3* Nicotinic Acetylcholine Receptors and an Agonist at the α7 Nicotinic Acetylcholine Receptor
Authors: Talka Reeta , Salminen Outi , Tuominen Raimo K. 
Published: September 2014

AbstractNicotine–methadone interactions have been studied in human beings and in various experimental settings regarding addiction, reward and pain. Most methadone maintenance treatment patients are smokers, and methadone administration has been shown to increase cigarette smoking. Previous in vitro studies have shown that methadone is a non‐competitive antagonist at rat α3β4 nicotinic acetylcholine receptors (nAChR) and an agonist at human α7 nAChRs. In this study, we used cell lines expressing human α4β2, α7 and α3* nAChRs to compare the interactions of methadone at the various human nAChRs under the same experimental conditions. A [3H]epibatidine displacement assay was used to determine whether methadone binds to the nicotinic receptors, and 86Rb+ efflux and changes in intracellular calcium [Ca2+]i were used to assess changes in the functional activity of the receptors. Methadone displaced [3H]epibatidine from nicotinic agonist‐binding sites in SH‐EP1‐hα7 and SH‐SY5Y cells, but not in SH‐EP1‐hα4β2 cells. The Ki values for methadone were 6.3 μM in SH‐EP1‐hα7 cells and 19.4 μM and 1008 μM in SH‐SY5Y cells. Methadone increased [Ca2+]i in all cell lines in a concentration‐dependent manner, and in SH‐EP1‐hα7 cells, the effect was more pronounced than the effect of nicotine treatment. In SH‐EP1‐hα4β2 cells, the effect of methadone was negligible compared to that of nicotine. Methadone pre‐treatment abolished the nicotine‐induced response in [Ca2+]i in all cell lines expressing nAChRs. In SH‐EP1‐hα4β2 and SH‐SY5Y cells, methadone had no effect on the 86Rb+ efflux, but it antagonized the nicotine‐induced 86Rb+ ion efflux in a non‐competitive manner. These results suggest that methadone is an agonist at human α7 nAChRs and a non‐competitive antagonist at human α4β2 and α3* nAChRs. This study adds further support to the previous findings that opioids interact with nAChRs, which may underlie their frequent co‐administration in human beings and might be of interest to the field of drug discovery.
Meta‐analysis based variable selection for gene expression data
Authors: Li Quefeng , Wang Sijian , Huang Chiang‐Ching , Yu Menggang , Shao Jun 
Published: September 2014

SummaryRecent advance in biotechnology and its wide applications have led to the generation of many high‐dimensional gene expression data sets that can be used to address similar biological questions. Meta‐analysis plays an important role in summarizing and synthesizing scientific evidence from multiple studies. When the dimensions of datasets are high, it is desirable to incorporate variable selection into meta‐analysis to improve model interpretation and prediction. According to our knowledge, all existing methods conduct variable selection with meta‐analyzed data in an “all‐in‐or‐all‐out” fashion, that is, a gene is either selected in all of studies or not selected in any study. However, due to data heterogeneity commonly exist in meta‐analyzed data, including choices of biospecimens, study population, and measurement sensitivity, it is possible that a gene is important in some studies while unimportant in others. In this article, we propose a novel method called meta‐lasso for variable selection with high‐dimensional meta‐analyzed data. Through a hierarchical decomposition on regression coefficients, our method not only borrows strength across multiple data sets to boost the power to identify important genes, but also keeps the selection flexibility among data sets to take into account data heterogeneity. We show that our method possesses the gene selection consistency, that is, when sample size of each data set is large, with high probability, our method can identify all important genes and remove all unimportant genes. Simulation studies demonstrate a good performance of our method. We applied our meta‐lasso method to a meta‐analysis of five cardiovascular studies. The analysis results are clinically meaningful.