bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of WNK3 Kinase Signaling Reduces Brain Damage and Accelerates Neurological Recovery After Stroke
Authors: Begum Gulnaz , Yuan Hui , Kahle Kristopher T. , Li Liaoliao , Wang Shaoxia , Shi Yejie , Shmukler Boris E. , Yang Sung-Sen , Lin Shih-Hua , Alper Seth L. , Sun Dandan 
Published: June 2015

Background and Purpose—

WNK kinases, including WNK3, and the associated downstream Ste20/SPS1-related proline-alanine–rich protein kinase (SPAK) and oxidative stress responsive 1 (OSR1) kinases, comprise an important signaling cascade that regulates the cation-chloride cotransporters. Ischemia-induced stimulation of the bumetanide-sensitive Na
+
-K
+
-Cl
−
cotransporter (NKCC1) plays an important role in the pathophysiology of experimental stroke, but the mechanism of its regulation in this context is unknown. Here, we investigated the WNK3-SPAK/OSR1 pathway as a regulator of NKCC1 stimulation and their collective role in ischemic brain damage.

Method—

Wild-type
WNK3
and
WNK3
knockout mice were subjected to ischemic stroke via transient middle cerebral artery occlusion. Infarct volume, brain edema, blood brain barrier damage, white matter demyelination, and neurological deficits were assessed. Total and phosphorylated forms of WNK3 and SPAK/OSR1 were assayed by immunoblotting and immunostaining. In vitro ischemia studies in cultured neurons and immature oligodendrocytes were conducted using the oxygen-glucose deprivation/reoxygenation method.

Results—

WNK3 knockout mice exhibited significantly decreased infarct volume and axonal demyelination, less cerebral edema, and accelerated neurobehavioral recovery compared with WNK3 wild-type mice subjected to middle cerebral artery occlusion. The neuroprotective phenotypes conferred by WNK3 knockout were associated with a decrease in stimulatory hyperphosphorylations of the SPAK/OSR1 catalytic T-loop and of NKCC1 stimulatory sites Thr
203
/Thr
207
/Thr
212
, as well as with decreased cell surface expression of NKCC1. Genetic inhibition of WNK3 or small interfering RNA knockdown of SPAK/OSR1 increased the tolerance of cultured primary neurons and oligodendrocytes to in vitro ischemia.

Conclusions—
These data identify a novel role for the WNK3-SPAK/OSR1-NKCC1 signaling pathway in ischemic neuroglial injury and suggest the WNK3-SPAK/OSR1 kinase pathway as a therapeutic target for neuroprotection after ischemic stroke.
31 May 2013 El Reno Tornadoes: Advantages of Rapid-Scan Phased-Array Radar Data from a Warning Forecaster’s Perspective*
Authors: Kuster Charles M. , Heinselman Pamela L. , Austin Marcus 
Published: June 2015

Abstract
On 31 May 2013, a supercell produced a tornado rated as 3 on the enhanced Fujita scale (EF3) near El Reno, Oklahoma, which was sampled by the S-band phased-array radar (PAR) at the National Weather Radar Testbed in Norman, Oklahoma. Collaboration with the forecaster who issued tornado warnings for the El Reno supercell during real-time operations focused the analysis on critical radar signatures frequently assessed during warning operations. The wealth of real-world experience provided by the forecaster, along with the quantitative analysis, highlighted differences between rapid-scan PAR data and the Weather Surveillance Radar-1988 Doppler located near Oklahoma City, Oklahoma (KTLX), within the context of forecast challenges faced on 31 May 2013. The comparison revealed that the 70-s PAR data proved most advantageous to the forecaster’s situational awareness in instances of rapid storm organization, sudden mesocyclone intensification, and abrupt, short-term changes in tornado motion. Situations where PAR data were most advantageous in the depiction of storm-scale processes included 1) rapid variations in mesocyclone intensity and associated changes in inflow magnitude; 2) imminent radar-indicated development of the short-lived (EF0) Calumet, Oklahoma, and long-lived (EF3) El Reno tornadoes; and 3) precise location and motion of the tornado circulation. As a result, it is surmised that rapid-scan volumetric radar data in cases like this would augment a forecaster’s ability to observe rapidly evolving storm features and deliver timely, life-saving information to the general public.
Effects of Remnant Tissue Preservation on Clinical and Arthroscopic Results After Anatomic Double-Bundle Anterior Cruciate Ligament Reconstruction
Authors: Kondo Eiji , Yasuda Kazunori , Onodera Jun , Kawaguchi Yasuyuki , Kitamura Nobuto 
Published: June 2015

Background: Clinical utility of remnant tissue preservation after single-bundle anterior cruciate ligament (ACL) reconstruction has not been established. In addition, no studies have evaluated the clinical utility of remnant preservation after anatomic double-bundle ACL reconstruction. Hypothesis: The study hypotheses were as follows: (1) Subjective and functional clinical results may be comparable between anatomic double-bundle reconstructions that preserve the remnant tissue and those that resect the remnant tissue, (2) postoperative knee stability and the second-look arthroscopic evaluation may be significantly more favorable with the remnant-preserving reconstruction, and (3) the degree of the initial graft coverage may significantly affect postoperative knee stability. Study Design: Cohort study; Level of evidence, 2. Methods: A total of 179 patients underwent anatomic double-bundle ACL reconstruction. Based on the Crain classification of ACL remnant tissue, 81 patients underwent the remnant-preserving procedure (group P) and the remaining 98 patients underwent the remnant-resecting procedure (group R). There were no differences between the 2 groups concerning all background factors, including preoperative knee instability and intraoperative tunnel positions. The patients were followed for 2 years or more. Results: The subjective and functional clinical results were comparable between the 2 reconstruction procedures. Side-to-side anterior laxity was significantly less ( P = .0277) in group P (0.9 mm) than in group R (1.5 mm). The pivot-shift test was negative in 89% of group P and 78% of group R patients; the result for group R was significantly lower ( P = .0460). In the arthroscopic observations, results for group P were significantly better than for group R concerning postoperative laceration and fibrous tissue coverage of the grafts ( P = .0479). Conclusion: Remnant preservation in anatomic double-bundle ACL reconstruction did not significantly improve subjective and functional results in the short-term evaluation, but it significantly improved postoperative knee stability. The degree of initial graft coverage significantly affected postoperative knee stability.
The role of AST-120 and protein-bound uremic toxins in irritable bowel syndrome: a therapeutic perspective
Authors: Mosińska Paula , Storr Martin , Fichna Jakub 
Published: June 2015

AST-120 (kremezin) exhibits its favourable effects in reducing the levels of renal toxins by selective adsorption of low molecular weight substances from the intestinal lumen. So far, a vast majority of studies were focused on the role of AST-120 in the treatment of chronic kidney diseases and cardiovascular disorders, and positive therapeutic effects of the agent have already been confirmed in clinical conditions. Up to the present, there are only a few studies regarding the role of AST-120 in irritable bowel syndrome (IBS). Compelling data suggest the ability of the compound to adsorb protein-bound uremic toxins and mast cell derived mediators and to modulate the farnesoid X receptor, which is a bile acid sensor indispensable for maintaining homeostasis in the intestine. In this review we focus on the actions of AST-120 on intestinal permeability, reduction of visceral sensitivity and alteration of gut motility. We also discuss whether AST-120 can mitigate common IBS symptoms, such as abdominal pain, bloating and malfunction of the colonic transit and thus improve the quality of life of patients with IBS.
A Low-Cost Inkjet-Printed Glucose Test Strip System for Resource-Poor Settings
Authors: Gainey Wilson Kayla , Ovington Patrick , Dean Delphine 
Published: June 2015

Background: The prevalence of diabetes is increasing in low-resource settings; however, accessing glucose monitoring is extremely difficult and expensive in these regions. Work is being done to address the multitude of issues surrounding diabetes care in low-resource settings, but an affordable glucose monitoring solution has yet to be presented. An inkjet-printed test strip solution is being proposed as a solution to this problem. Methods: The use of a standard inkjet printer is being proposed as a manufacturing method for low-cost glucose monitoring test strips. The printer cartridges are filled with enzyme and dye solutions that are printed onto filter paper. The result is a colorimetric strip that turns a blue/green color in the presence of blood glucose. Results: Using a light-based spectroscopic reading, the strips show a linear color change with an R2 = .99 using glucose standards and an R2 = .93 with bovine blood. Initial testing with bovine blood indicates that the strip accuracy is comparable to the International Organization for Standardization (ISO) standard 15197 for glucose testing in the 0-350 mg/dL range. However, further testing with human blood will be required to confirm this. A visible color gradient was observed with both the glucose standard and bovine blood experiment, which could be used as a visual indicator in cases where an electronic glucose meter was unavailable. Conclusions: These results indicate that an inkjet-printed filter paper test strip is a feasible method for monitoring blood glucose levels. The use of inkjet printers would allow for local manufacturing to increase supply in remote regions. This system has the potential to address the dire need for glucose monitoring in low-resource settings.
Sunitinib therapy for metastatic renal cell carcinoma: A urologist’s perspective
Authors: Darrad M , Wilson R 
Published: June 2015

Objective: Sunitinib is seldom initiated and managed by urologists, and patients with metastatic renal cell carcinoma are often referred to geographically distant centres. We present the outcomes of patients on sunitinib therapy in a single-centre urological department. To the best of our knowledge, we provide one of the only urology-led services for these patients in the United Kingdom. Method: Between August 2009 and January 2013, an unselected group of patients with metastatic renal cell carcinoma receiving sunitinib therapy were analysed. Twenty-eight patients (20 males, eight females) with an average age of 64.8 years were included in our study. Results: Patients were seen on average 10.8 days following MDT and were given fortnightly appointments to monitor progress. There were 176 cycles of sunitinib delivered. A total of 39.3% patients required at least one dose reduction. The commonest adverse events were diarrhoea (71%), mucositis (46%) and hand-foot syndrome (36%). Partial response and stable disease were observed in 27.1% and 45.8% of patients, respectively. Progression-free survival (PFS) and overall survival (OS) were 8.6 months and 15.2 months, respectively. Compared to the literature, there were lower rates of haematological adverse events in our study; however, PFS and OS, response rates and dose reductions were comparable. Conclusions: This study highlights that such patients can be safely and effectively managed in a local hospital setting with a dedicated urological team, allowing for better continuity of care.
Secondary findings and carrier test frequencies in a large multiethnic sample
Authors: Gambin Tomasz , Jhangiani Shalini N. , Below Jennifer E. , Campbell Ian M. , Wiszniewski Wojciech , Muzny Donna M. , Staples Jeffrey , Morrison Alanna C. , Bainbridge Matthew N. , Penney Samantha , McGuire Amy L. , Gibbs Richard A. , Lupski James R. , Boerwinkle Eric 
Published: June 2015

Abstract
Background
Besides its growing importance in clinical diagnostics and understanding the genetic basis of Mendelian and complex diseases, whole exome sequencing (WES) is a rich source of additional information of potential clinical utility for physicians, patients and their families. We analyzed the frequency and nature of single nucleotide variants (SNVs) considered secondary findings and recessive disease allele carrier status in the exomes of 8554 individuals from a large, randomly sampled cohort study and 2514 patients from a study of presumed Mendelian disease having undergone WES.

Methods
We used the same sequencing platform and data processing pipeline to analyze all samples and characterized the distributions of reported pathogenic (ClinVar, Human Gene Mutation Database (HGMD)) and predicted deleterious variants in the pre-specified American College of Medical Genetics and Genomics (ACMG) secondary findings and recessive disease genes in different ethnic groups.

Results
In the 56 ACMG secondary findings genes, the average number of predicted deleterious variants per individual was 0.74, and the mean number of ClinVar reported pathogenic variants was 0.06. We observed an average of 10 deleterious and 0.78 ClinVar reported pathogenic variants per individual in 1423 autosomal recessive disease genes. By repeatedly sampling pairs of exomes, 0.5 % of the randomly generated couples were at 25 % risk of having an affected offspring for an autosomal recessive disorder based on the ClinVar variants.

Conclusions
By investigating reported pathogenic and novel, predicted deleterious variants we estimated the lower and upper limits of the population fraction for which exome sequencing may reveal additional medically relevant information. We suggest that the observed wide range for the lower and upper limits of these frequency numbers will be gradually reduced due to improvement in classification databases and prediction algorithms.
Research roundup: June 2015
Authors: Greener Mark 
Published: June 2015

In brief ▪ Opioids, selective serotonin reuptake inhibitors, antipsychotics and, possibly, tricyclic antidepressants seem to increase the risk of falls and hip fractures among elderly people ▪ Measles, mumps and rubella vaccination does not seem to increase the likelihood of developing autism-spectrum disorders, even in children at high risk ▪ Statins may reduce mortality from lung cancer ▪ E-cigarettes do not seem to increase the likelihood of quitting smoking; a single vaping session impairs the cough reflex ▪ Aspirin may slow the progression of early emphysema
Retention, separation selectivity and system efficiency of selected basic psychotropic drugs on different RPLC columns
Authors: Petruczynik Anna , Wróblewski Karol , Dzioba Kamil , Waksmundzka-Hajnos Monika 
Published: June 2015

Abstract
Retention parameters of psychotropic drug
standards were determined on different columns,
i.e., Octadecyl silica, Phenyl, Phenyl-Hexyl, Polar
Reverse Phase, Pentafluorophenyl, and Cyanopropyl
using aqueous eluent systems containing methanol or
acetonitrile as organic modifiers, acetate buffer at pH 3.5
and addition of silanol blocker − diethylamine (DEA).

The retention, separation selectivity, and sequence of
elution were different when using eluents containing various
organic modifiers. The significant differences were observed
in retention parameters with a change of the used stationary
phase. The various properties of stationary phases resulted
in differences in analyte retention, peaks shape, systems
efficiency and separation selectivity. The best shape of
peaks were on Cyanopropyl (CN) column and the highest
efficiency for most investigated psychotropic drugs were
obtained on Phenyl-Hexyl and Polar RP columns.
Multidisciplinary management of giant genital tract venous malformations during pregnancy: case report and review of the literature
Authors: Hubinont Corinne , Roelants Fabienne , Boon Laurence , Minlangu Ana Bila , Hermans Cédric , Debiève Frédéric 
Published: June 2015

Abstract
Malformation affecting the genital tract is a rare condition. Enlargement of the mass and specific complications may occur following hemodynamic changes associated with pregnancy. A 25-year-old pregnant primigravida affected by an extended vulvar and thigh venous malformation with localised intravascular consumptive coagulopathy was referred to our hospital. Her antenatal care was uneventful until term. She underwent an elective caesarean section because the venous malformation location precluded vaginal birth. At 3 days postpartum, she suffered life-threatening bleeding in the abdominal cavity with disseminated intravascular coagulation requiring multiple blood and platelets transfusions as well as transarterial embolisation. The patient recovered and was discharged after 20 days. Specific counselling and management of pregnant patients with large vascular malformations is essential as it is associated with increased complications rate such as peripartal thrombosis and haemorrhages. These patients should be referred to tertiary centres and should be managed by a multidisciplinary team including an obstetrician, anaesthesiologist, haematologist, interventional radiologist and plastic surgeon.
The First Catholic Bible in Chinese: Gabriele Allegra and His Translation
Authors: De Gruttola Raissa 
Published: June 2015

Abstract
Christian missionaries play an important role in the history of the relationship between China and Europe. Their presence in China has been widely explored, but little attention has been paid to the role played by the Bible in their preaching.
From 13th to 19th century, although they did not translate the Bible, Catholic missionaries preached the Gospel orally or with catechisms. On the other hand, the Protestant missionaries had published many version of the Chinese Bible throughout the 19th century.
It was only in the 20th century that the Franciscan friar Gabriele Allegra decided to go to China as a missionary to translate the Holy Scriptures into Chinese. He arrived in China in 1931 and translated from 1935 to 1961. He also founded a biblical study centre to prepare expert scholars to collaborate in the Bible translation. Allegra and his colleagues completed the translation in 1961, and the first complete single-volume Catholic Bible in Chinese was published in 1968.
After presenting the historical background of Allegra’s activity, a textual analysis of some passages of his translation will be presented, emphasizing the meanings of the Chinese words he chose to use to translate particular elements of Christian terminology. This study will verify the closeness of the work by Allegra to the original Greek text and the validity of some particular translation choices.
Contemporary Afghanistan Outlook: Between Counterinsurgency and Search of the Social Contract
Authors: Bivainis Andrius 
Published: June 2015

Abstract
This article is based on reassessment of the contemporary results of counterinsurgency and nation-building in Afghanistan. Nation-building initiatives have been started in the country since the Bonn agreement in December 2001. This agreement brought into reality the current governing system of Afghanistan. Counterinsurgency in Afghanistan has been initiated in full mode since 2009 after a sound success on Iraqi frontier. However, each operational area is bringing its own specifics into play. The same was with Afghanistan. The newly established constitutional presidential republic has faced with inheritance of unresolved sensitive ethnical identity issues, confrontation between different groups for self-governing authority and security of essential resources. These preconditions have brought a diversified and even confrontational social environment into reality. Prolonged military operations in Afghanistan could show that diversified social environment and misevaluated cultural heritage has led to misleading assumptions that centralized presidential governing system could become an effective ruling model for post-Taliban country.
One of the key notions of this article is that historical lessons taught by long years of colonialist rule in Afghanistan has not been learned and misevaluation of diversified and confrontational local entities has brought another historical lesson of Afghan tribal resistance. More than that, diversified and confrontational entities of Afghanistan have not been a favorable subject for possible social contract. The term social contract was introduced as explanatory method of national political behavior and systemic structure by Jean Jacques Rousseau in 18th century Europe. Afghan society has become the subject to this model of political philosophy only as counterinsurgency campaign gained full capabilities around 2009. Reassessment of long term nation building efforts in this article is based on evaluation of Afghan social contract’s progress.
Flexible Reference Frames for Grasp Planning in Human Parietofrontal Cortex
Authors: Leoné Frank T. M. , Monaco Simona , Henriques Denise Y. P. , Toni Ivan , Medendorp W. Pieter 
Published: June 2015

AbstractReaching to a location in space is supported by a cortical network that operates in a variety of reference frames. Computational models and recent fMRI evidence suggest that this diversity originates from neuronal populations dynamically shifting between reference frames as a function of task demands and sensory modality. In this human fMRI study, we extend this framework to nonmanipulative grasping movements, an action that depends on multiple properties of a target, not only its spatial location. By presenting targets visually or somaesthetically, and by manipulating gaze direction, we investigate how information about a target is encoded in gaze- and body-centered reference frames in dorsomedial and dorsolateral grasping-related circuits. Data were analyzed using a novel multivariate approach that combines classification and cross-classification measures to explicitly aggregate evidence in favor of and against the presence of gaze- and body-centered reference frames. We used this approach to determine whether reference frames are differentially recruited depending on the availability of sensory information, and where in the cortical networks there is common coding across modalities. Only in the left anterior intraparietal sulcus (aIPS) was coding of the grasping target modality dependent: predominantly gaze-centered for visual targets and body-centered for somaesthetic targets. Left superior parieto-occipital cortex consistently coded targets for grasping in a gaze-centered reference frame. Left anterior precuneus and premotor areas operated in a modality-independent, body-centered frame. These findings reveal how dorsolateral grasping area aIPS could play a role in the transition between modality-independent gaze-centered spatial maps and body-centered motor areas.
Concomitant Transapical Transcatheter Valve Implantations: Edwards Sapien Valve for Severe Mitral Regurgitation in a Patient with Failing Mitral Bioprostheses and JenaValve for the Treatment of Pure Aortic Regurgitation
Authors: Aydin Unal , Gul Mehmet , Aslan Serkan , Akkaya Emre , Yildirim Aydin 
Published: June 2015

Transcatheter valve implantation is a novel interventional technique, which was developed as an alternative therapy for surgical aortic valve replacement in inoperable patients with severe aortic stenosis. Despite limited experience in using transcatheter valve implantation for mitral and aortic regurgitation, transapical transcatheter aortic valve implantation and valve-in-valve implantation for degenerated mitral valve bioprosthesis can be performed in high-risk patients who are not candidates for conventional replacement surgery. In this case, we present the simultaneous transcatheter valve implantation via transapical approach for both degenerated bioprosthetic mitral valve with severe regurgitation and pure severe aortic regurgitation.