bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maternal and health care determinants of preconceptional use of folic acid supplementation in France: results from the 2010 National Perinatal Survey
Authors: Tort J , Lelong N , Prunet C , Khoshnood B , Blondel B 
Published: September 2013

ObjectivesTo estimate the national prevalence and analyse the factors associated with preconceptional folic acid supplementation, including maternal sociodemographic characteristics, region of residence, birth control use and chronic diseases requiring medical care before conception.DesignCross‐sectional population‐based study.SettingAll maternity units in France.PopulationA nationally representative sample of women giving birth in 2010 (n = 12,646).MethodsData came from mothers' interviews 2–3 days after delivery. Statistical analyses included multivariable logistic regressions.Main outcome measureFolic acid supplementation starting at least 1 month before conception.Results14.8% (95% confidence interval [95% CI] 14.2–15.4) of women used folic acid before pregnancy; this percentage varied from 10.4% to 18.7% across regions. Supplementation was more frequent in primiparae, French citizens, women with higher educational levels and those needing medical monitoring or treatment before conception. Women who stopped contraception to become pregnant (75% of our population) used folic acid more often (intrauterine device or implant: 19%, pill: 17%, other methods which did not need medical monitoring: 17%) than other women (7%). The adjusted odds ratios were 3.3 (95% CI 2.6–4.3) for intrauterine device and implant, 2.2 (95% CI 1.8–2.6) for pill and 1.9 (95% CI 1.5–2.4) for other methods, compared with women who did not use birth control.ConclusionThe absence of preconceptional folic acid supplementation for most women, even those needing consultations with healthcare professionals before pregnancy, shows that campaigns to promote folic acid supplementation should address not only women but also healthcare professionals involved in birth control and obstetric care before pregnancy.
Mandatory Disclosure, Voluntary Disclosure, and Stock Market Liquidity: Evidence from the EU Bank Stress Tests
Authors: BISCHOF JANNIS , DASKE HOLGER 
Published: September 2013

ABSTRACTWe use the EU stress tests and the Eurozone sovereign debt crisis to study the consequences of supervisory disclosure of banks’ sovereign risk exposures. We test the idea that a mandatory one‐time disclosure induces an increase in voluntary disclosures about sovereign risk in the following periods and, through the shift in the voluntary disclosure equilibrium, increases the liquidity of banks’ shares. First, we find that the timing and content of different mandatory disclosure events helps explain the levels of stress‐test banks’ voluntary disclosures about sovereign risk. Second, although the bid‐ask spreads of stress test participants generally increased after the mandatory stress test in 2011, our results suggest that the decrease in market liquidity is entirely attributable to those stress‐test participants that did not commit to voluntarily maintaining the disclosures of sovereign risk exposure.
FACE‐R—A 3D Database of 400 Living Individuals' Full Head CT‐ and Face Scans and Preliminary GMM Analysis for Craniofacial Reconstruction
Authors: Kustár Agnes , Forró Laszlo , Kalina Ildiko , Fazekas Ferenc , Honti Szabolcs , Makra Szabolcs , Friess Martin 
Published: September 2013

AbstractIn the past, improvements in craniofacial reconstructions (CFR) methodology languished due to the lack of adequate 3D databases that were sufficiently large and appropriate for 3‐dimensional shape statistics. In our study, we created the “FACE‐R” database from CT records and 3D surface scans of 400 clinical patients from Hungary, providing a significantly larger sample that was available before. The uniqueness of our database is linking of two data types that makes possible to investigate the bone and skin surface of the same individual, in upright position, thus eliminating many of the gravitational effects on the face during CT scanning. We performed a preliminary geometric morphometric (GMM) study using 3D data that produces a general idea of skull and face shape correlations. The vertical position of the tip of the (soft) nose for a skull and landmarks such as rhinion need to be taken into account. Likewise, the anterior nasal spine appears to exert some influence in this regard.
Sex differences in the influence of social context, salient social stimulation and amphetamine on ultrasonic vocalizations in prairie voles
Authors: MA Sean T. , RESENDEZ Shanna L. , ARAGONA Brandon J. 
Published: September 2013

AbstractPrairie voles (Microtus ochrogaster) are a socially monogamous rodent species and their cooperative behaviors require extensive communication between conspecifics. Rodents use ultrasonic vocalizations (USVs) to communicate and because a prairie vole breeder pair must engage in extensive cooperation for successful reproduction, auditory communication may be critical for this species. Therefore, we sought to characterize USVs in adult male and female prairie voles, and to determine how these calls are influenced by social context, salient social stimuli and the psychostimulant drug of abuse amphetamine (AMPH). Here, we characterize prairie vole USVs by showing the range of frequencies of prairie vole USVs, the proportion of various call types, how these call types compare between males and females, and how they are influenced by social stimulation and AMPH. AMPH caused a robust increase in the number of USVs in both males and females and there was a dramatic sex difference in the complexity of call structures of AMPH‐induced USVs, with males emitting more elaborate calls. Moreover, we show that novel (i.e. salient) social cues evoked differential increases in USVs across sex, with males showing a much more robust increase in USV production, both with respect to the frequency and complexity of USV production. Exposure to an estrous female in particular caused an extraordinary increase in USVs in male subjects. These data suggest that USVs may be a useful measure of social motivation in this species, including how social behaviors can be impacted by drugs of abuse.
Physicochemical Characterization and Sensory Analysis of Yeast‐leavened and Sourdough Soy Breads
Authors: Yezbick Gabrielle , Ahn‐Jarvis Jennifer , Schwartz Steven J. , Vodovotz Yael 
Published: September 2013

AbstractSourdough fermentation has been shown to have numerous beneficial effects on bread quality, and nutritionally enhance soy‐supplemented bread by altering isoflavone chemical forms. Given this, the objective of this study was to compare the loaf quality and shelf life of sourdough and yeast‐leavened soy breads by various physical, thermal, and sensorial methods, and to assess the effects of fermentation by various microorganisms on isoflavone profile in dough and breads using high‐performance liquid chromatography analysis. Sourdough fermentation yielded a less extensible dough compared to yeast‐leavened soy dough (P < 0.001), and resulted in a harder bread crumb (P < 0.05) and lighter crust color (P < 0.001), compared to yeast‐leavened soy bread (Y‐B). Sensory analysis revealed a significantly higher overall liking of Y‐B compared to sourdough soy bread (SD‐B) (P < 0.001). Segmentation analysis of the cohort suggests that overall liking and bread consumption frequency may be determinants of Y‐B or SD‐B preference. SD‐B and Y‐B exhibited similar shelf‐life properties. Despite significantly different enthalpies associated with the melting of amylose‐lipid complexes, thermal analysis of the 2 soy breads stored for 10 d (ambient conditions) demonstrated no significant difference in water distribution and starch retrogradation (P < 0.05). Lastly, SD‐B was determined to have 32% of total isoflavones occurring in the aglycone form compared to 17% in Y‐B. These findings warrant further investigation of sourdough fermentation as a processing technique for quality and nutritional enhancement of soy‐based baked goods.
Botryticidal Activity of Nanosized Silver‐Chitosan Composite and Its Application for the Control of Gray Mold in Strawberry
Authors: Moussa Shaaban H. , Tayel Ahmed A. , Alsohim Abdullah S. , Abdallah Reda R. 
Published: September 2013

AbstractBotrytis cinerea Pers, the gray mold fungus, is among the most dangerous plant pathogens that cause great losses in agricultural crops. The botryticidal activities of nanosized silver (nano Ag), fungal chitosan (CTS) irradiated fungal chitosan (IrCTS), and nano Ag‐IrCTS composite were investigated. All of the examined materials exhibited powerful antifungal activity against B. cinerea. The most effective compound was nano Ag‐IrCTS composite with a minimal inhibitory concentration of 125 μg/mL. The microscopic examination, of treated B. cinerea with the composite, revealed that an obvious alteration in mycelial shape was appeared accompanied and moderate lysis in fungal hyphae. With the prolongation of treatment, most of the fungal mycelia were lysed into small and elastic fragments. The consequence of strawberries coating, with antifungal composite based solution, was the disappearance of gray mold infection signs in 90% of the contaminated fruits after 7 d of storage, treated fruits had a fresh‐like appearance at the end of storage. Coating with nano Ag‐IrCTS solution could be highly recommended regarding its efficiency in prohibiting B. cinerea growth, preventing gray mold decay and enhancing the overall quality of coated strawberry fruits.
Phytochemical Analysis and Anti‐inflammatory Potential of Hyphaene thebaica L. Fruit
Authors: Farag Mohamed A. , Paré Paul W. 
Published: September 2013

AbstractMetabolite profiling and biological activity are reported from organic and aqueous extracts of the fruit from the desert palm Hyphaene thebaica. Phenolics and oxylipids profiles were determined using UPLC‐PDA‐TOF (ultra performance‐photodiode array‐time of flight) high‐resolution mass spectrometry in order to obtain the molecular formula and exact mass Under optimized conditions, 17 compounds were simultaneously identified and quantified including 2 cinnamic acid derivatives, 5 flavonoids, 6 fatty acids, 2 sphingolipids, a lignan, and a stilbene. Sugars composition in the fruit was characterized and quantified by 1H‐NMR (nuclear magnetic resonance) with sucrose detected as the major component in fruit at a level of 219 mg/g. Fruit organic extracts anti‐inflammatory potential was assessed in vitro by cyclooxygenase‐1 enzyme inhibition.
Chemical Treatments for Reducing the Yellow Discoloration of Channel Catfish (Ictalurus punctatus) Fillets
Authors: Li Yilin , Liu Shaoyang , Cline David , Chen Shengshun , Wang Yifen , Bell Leonard N. 
Published: September 2013

AbstractThe effect of chemical pretreatments on the color and carotenoid content of yellow discolored channel catfish fillets was studied. The color and carotenoid content of the fillets were analyzed by the L*a*b* color system and high‐performance liquid chromatography, respectively. Untreated fillets turned more yellow and darker after 12‐d storage. Sodium bicarbonate had a beneficial effect on reducing the degree of yellowness, but the fillets still turned darker after storage. Sodium bisulfite gave the best results with fillets becoming less yellow and brighter after storage. Ascorbic acid, butylated hydroxyanisole, citric acid, and sodium metabisulfite were not successful at reducing the discoloration. The sum of carotenoid contents of untreated fillets decreased significantly (P < 0.05) during storage as compared to the fresh fillets. However, the sum of the carotenoid contents of fillets treated by various chemicals was not significantly (P > 0.05) different from the fresh or untreated fillets. The appearance of catfish fillets may be improved during storage by pretreating with sodium bisulfite.
Phase II trial of paclitaxel‐carboplatin with intercalated gefitinib for untreated, epidermal growth factor receptor gene mutation status unknown non‐small cell lung cancer
Authors: Yang Jianliang , Shi Yuankai , Zhang Xiangru , Xu Jianping , Wang Bin , Hao Xuezhi , Li Junling , Yan Wang 
Published: September 2013

AbstractBackgroundThis study was conducted to evaluate the efficacy and safety of paclitaxel‐carboplatin combined with intercalated gefitinib in patients with advanced, untreated, nonsquamous non‐small cell lung cancer.MethodsA total of 29 patients were enrolled in the study. All patients were Chinese, with a histology type of adenocarcinoma, without a smoking history, and as a result of the limited tissue sample, an epidermal growth factor receptor (EGFR) mutation test could not be performed. All patients received chemotherapy of paclitaxel‐carboplatin every 21 days for four cycles, and gefitinib (250 mg/day) was administered on days eight to 17 of the chemotherapy cycle. If the patient responded to chemotherapy, maintenance therapy of 250mg of gefitinib could be administered daily.ResultsAll of the 29 patients received at least one cycle of chemotherapy and gefitinib, and 25 patients received four cycles of therapy. Eighteen patients selected maintenance therapy with gefitinib. The objective response rate was 74.1% (95% confidence interval, 53.7% to 88.9%). No complete response was achieved. The median progression‐free survival was 16 months, however, the median overall survival was not available by the conclusion of the study. The major adverse event was hematologic toxicity.ConclusionsThe regimen of paclitaxel‐carboplatin combined with intercalated gefitinib showed a high response rate and a favorable safety profile. Gefitinib maintenance therapy was proven to be beneficial. This study proposes a good pattern of chemotherapy combined with EGFR tyrosine kinase inhibitors.
Altered cingulate and insular cortex activation during risk‐taking in methamphetamine dependence: losses lose impact
Authors: Gowin Joshua L. , Stewart Jennifer L. , May April C. , Ball Tali M. , Wittmann Marc , Tapert Susan F. , Paulus Martin P. 
Published: September 2013

AbstractAimsTo determine if methamphetamine‐dependent (MD) individuals exhibit behavioral or neural processing differences in risk‐taking relative to healthy comparison participants (CTL).DesignThis was a cross‐sectional study comparing two groups' behavior on a risk‐taking task and neural processing as assessed using functional magnetic resonance imaging (fMRI).SettingsThe study was conducted in an in‐patient treatment center and a research fMRI facility in the United States.ParticipantsSixty‐eight recently abstinent MD individuals recruited from a treatment program and 40 CTL recruited from the community completed the study.MeasurementsThe study assessed risk‐taking behavior (overall and post‐loss) using the Risky Gains Task (RGT), sensation‐seeking, impulsivity and blood–oxygenation‐level‐dependent activation in the brain during the decision phase of the RGT.FindingsRelative to CTL, MD displayed decreased activation in the bilateral rostral anterior cingulate cortex (ACC) and greater activation in the left insula across risky and safe decisions (P < 0.05). Right mid‐insula activation among CTL did not vary between risky and safe decisions, but among MD it was higher during risky relative to safe decisions (P < 0.05). Among MD, lower activation in the right rostral ACC (r = −0.39, P < 0.01) and higher activation in the right mid‐insula (r = 0.35, P < 0.01) during risky decisions were linked to a higher likelihood of choosing a risky option following a loss.ConclusionsMethamphetamine‐dependent individuals show disrupted risk‐related processing in both anterior cingulate and insula, brain areas that have been implicated in cognitive control and interoceptive processing. Attenuated neural processing of risky options may lead to risk‐taking despite experiencing negative consequences.
Secretory Factors From Rat Adipose Tissue Explants Promote Adipogenesis and Angiogenesis
Authors: Li Jie , Qiao Xiangchen , Yu Mei , Li Feng , Wang Hang , Guo Weihua , Tian Weidong 
Published: September 2013

AbstractAdipogenic differentiation of adipose‐derived stem cells (ASCs) is known to be affected by many promoting and inhibiting factors, and correlated with surrounding cells and extracellular matrix. However, few studies have evaluated the effect of secreted biological molecules from adipose tissue explants on the growth of ASCs. In this study, ASCs were isolated and the secretory factors from adipose tissue explants (SFAE) were prepared from adipose tissue explants. The multilineage differentiation potential of ASCs was determined using different inductive media. The influence of SFAE on the colony formation and proliferation of ASCs was determined using colony‐forming efficiency assay and Cell Counting Kit‐8 assay, respectively. Real‐time polymerase chain reaction and Western blot were performed to analyze the beneficial effect of SFAE on the adipogenesis and angiogenesis of ASCs. Utilizing gelatin scaffold, the influence of SFAE on ASCs was investigated in vivo. Subsequently, cell/gelatin constructs were cultured in a subcutaneous pocket on a rat dorsum for 2 and 9 weeks, and the resultant samples were histologically evaluated. Results showed that ASCs derived from adipose explants can differentiate along multiple lineages in vitro. Utilizing SFAE, the proliferation and colony‐forming efficiency of ASCs were inhibited, while the expression of adipogenesis markers such as C/EBPβ, PPARγ2, and LPL, as well as angiogenesis factor VEGF‐A were promoted. Moreover, the beneficial effect of SFAE on adipogenesis was revealed in vivo. In conclusion, our results suggested that SFAE has beneficial influence on adipogenesis and angiogenesis both in vitro and in vivo.
Clinical Results and Pump Analysis of the Gyro Pump for Long‐Term Extracorporeal Life Support
Authors: Terasaki Takamitsu , Takano Tamaki , Michinaga Yuuki , Yokokawa Michihiro , Wada Yuko , Seto Tatsuichirou , Fukui Daisuke , Amano Jun 
Published: September 2013

AbstractRescuing patients in severe cardiac failure with extracorporeal support remains challenging. The Gyro pump is a centrifugal blood pump and was now used for cardiopulmonary bypass, although it was originally developed for long‐term cardiac assist. Little is known about clinical experiences using this pump. Here, we report on the clinical results of long‐term extracorporeal life support for over 4 days using the Gyro pump with Excelung, a hollow fiber oxygenator coated with silicone and heparin. Seven patients underwent extracorporeal life support with 15 pump and oxygenator combinations. Gyro and Excelung were used for venoarterial extracorporeal support in six patients and for right ventricular support in one patient. Patient characteristics, pump driving conditions, and blood chemistry were obtained retrospectively. All pumps were subsequently disassembled and examined macroscopically, with 6 of 15 pumps also examined by scanning electron microscopy (SEM). The patient mortality rate was 57.1%. Mean duration of support was 10.5 ± 7.2 days per pump and oxygenator combination. Lactate dehydrogenase and aspartate aminotransferase were generally maintained below 1000 and 100 IU/L, respectively, after the first 4 days of pump driving. Thrombi were found in two pumps, one used without anticoagulation and the other driven at a very slow rotational speed. SEM revealed no wear in the male bearings and very low wear and deformation (0.02 ± 0.03 mm) in the female bearings. The combination of Gyro and Excelung may be applicable for long‐term biventricular and right ventricular support, although proper anticoagulation should be administrated to avoid thrombus formation inside the pump.
The influence of individual, group, and relative self‐esteem on outcome for patients undergoing group cognitive‐behavioural therapy treatment
Authors: Parker Thomas J. , Page Andrew C. , Hooke Geoff R. 
Published: September 2013

ObjectivesDespite a strong association between individual self‐esteem and treatment outcome in group cognitive‐behavioural therapy (GCBT), no study has investigated how patient outcomes might be influenced by an individual's self‐esteem relative to other group members.DesignThe study comprised a retrospective examination of patients' data and used a multiple regression analysis to identify predictors of treatment outcome. Patients' pre‐treatment self‐esteem scores were assessed on a continuum and assigned to be low, medium, or high. Therapy groups were assigned to be either low, balanced or high self‐esteem groups based on averaged self‐esteem scores of participants.MethodsIn this study, 3,878 patients who had completed a 10‐day intensive cognitive behavioural group therapy programme at a private psychiatric facility were included in the study. The Rosenberg Self‐Esteem measure was chosen to assess self‐esteem. The three subscales of the Depression Anxiety Stress Scales were used as the outcome measures.ResultsPatient outcomes were influenced by pre‐treatment self‐esteem scores, such that higher initial self‐esteem was associated with better treatment outcomes. Low group self‐esteem was predictive of significantly better outcomes for depression, relative to higher self‐esteem groups. Additionally, the combined influence of high individual self‐esteem and low group self‐esteem was associated with significantly enhanced depression improvement.ConclusionsHigh self‐esteem patients perform better on outcome measures following completion of GCBT. Low self‐esteem groups show greater improvement in depression symptoms. Similar results for depression are achieved when patients with high self‐esteem complete treatment in low self‐esteem groups.Practitioner points
Patients with higher pre‐treatment self‐esteem perform better on outcome measures compared to individuals with lower self‐esteem.
Low self‐esteem groups perform better with respect to depression improvement. Similar results are found when high self‐esteem patients complete treatment in low self‐esteem groups.
The study was a retrospective examination of patients' data and not a manipulation study; therefore, cause and effect relationships were not able to be determined.
The generalizability of the finding to patients in other health settings remains to be determined.
Single‐cell PCR analysis of murine embryonic stem cells cultured on different substrates highlights heterogeneous expression of stem cell markers
Authors: Franzin Chiara , Piccoli Martina , Serena Elena , Bertin Enrica , Urbani Luca , Luni Camilla , Pasqualetto Valerie , Eaton Simon , Elvassore Nicola , De Coppi Paolo , Pozzobon Michela 
Published: September 2013

Background InformationIn the last few years, recent evidence has revealed that inside an apparently homogeneous cell population there indeed appears to be heterogeneity. This is particularly true for embryonic stem (ES) cells where markers of pluripotency are dynamically expressed within the single cells. In this work, we have designed and tested a new set of primers for multiplex PCR detection of pluripotency markers expression, and have applied it to perform a single‐cell analysis in murine ES cells cultured on three different substrates that could play an important role in controlling cell behaviour and fate: (i) mouse embryonic fibroblast (MEF) feeder layer, as the standard method for ES cells culture; (ii) Matrigel coating; (iii) micropatterned hydrogel.ResultsCompared with population analysis, using a single‐cell approach, we were able to evaluate not only the number of cells that maintained the expression of a specific gene but, most importantly, how many cells co‐expressed different markers. We found that micropatterned hydrogel seems to represent a good alternative to MEF, as the expression of stemness markers is better preserved than in Matrigel culture.ConclusionsThis single‐cell assay allows for the assessment of the stemness maintenance at a single‐cell level in terms of gene expression profile, and can be applied in stem cell research to characterise freshly isolated and cultured cells, or to standardise, for instance, the method of culture closely linked to the transcriptional activity and the differentiation potential.