bluuebunny/crossref_metadata_2025_split huggingface dataset:
A positive Link Between Male Testosterone and Spacing Behavior in Pair‐Bonded California Mice
Authors: Gleason Erin D. , Marler Catherine A. 
Published: September 2012

AbstractThe effects of testosterone (T) on male sexual behavior are well established, but its roles in several associated social behaviors such as pair‐bonding and paternal behavior are diverse. Recently, we reported that male T in response to pairing with a female predicts future paternal behavior in the monogamous and biparental California mouse (Peromyscus californicus). Here, we examined whether T in response to pairing is also associated with spacing behavior between mates under laboratory conditions and if these behavioral patterns are continued during pup care. Pairs of California mice were observed throughout their pair bond before and again after pups were born. We found that males with higher T post‐pairing remained closer to their mates in the weeks before pups were born, and pairs with higher proximity measures during the pair bond spent more time caring for pups together. Overall, these results suggest a similar mechanism for spacing behavior across distinct phases of the pair bond.
Harvest models and stock co‐occurrence: probabilistic methods for estimating bycatch
Authors: Drake D Andrew R , Mandrak Nicholas E 
Published: September 2012

AbstractA primary goal of ecosystem‐based fishery management is to reduce non‐target stock impacts, such as incidental harvest, during targeted fisheries. Quantifying incidental harvest has generally incorporated fishery‐dependent catch data, yet such data may be biased by gear non‐retention, observation difficulties, and non‐random harvest patterns that collectively lead to an impartial understanding of non‐target stock capture. To account for such issues and explicitly recognize the combined influence of ecological and harvest factors contributing to incidental capture within targeted fisheries, we demonstrate a probabilistic modelling framework that incorporates: (i) background rates of target and non‐target stock co‐occurrence as the primary ecological basis for incidental harvest; (ii) the probability of harvesting at localities exhibiting co‐occurrences; (iii) the probability of selecting for non‐target species with fishery gear; and, (iv) as a function of harvest effort, the overall probability of incidental capture for any non‐target stock contained in the species pool available for harvest. To illustrate application of the framework, simulation models were based on fishery‐independent data from a freshwater fishery in Ontario, Canada. Harvest simulations of empirical stock data indicated that greatest species‐specific capture values were over 4000 times more likely than for species with lowest values, indicating highly variable capture probabilities because of the combined influence of stock heterogeneity and harvest dynamics. Estimated bycatch–effort relationships will allow forecasting incidental harvest on the basis of effort to evaluate future shifts in fishing activity against specific ecosystem‐based fishery management objectives, such as reducing the overall probability of bycatch while maintaining target landings.
Oxidative capacities of cardiac and skeletal muscles of heart transplant recipients: mitochondrial effects of cyclosporin‐A and its vehicle Cremophor‐EL
Authors: N'Guessan Benoit Banga , Sanchez Hervé , Zoll Joffrey , Ribera Florence , Dufour Stéphane , Lampert Eliane , Kindo Michel , Geny Bernard , Ventura‐Clapier Renée , Mettauer Bertrand 
Published: September 2012

AbstractChronic immunosuppressive treatment was suspected to alter maximal muscle oxidative capacity (Vmax) of heart transplant recipients, leading to a limitation of their exercise tolerance. It remains undefined whether the mitochondrial respiratory chain (MRC) of right ventricle (RV) and vastus lateralis (VL) muscles were altered by immunosuppressants and/or their vehicles. Vmax was measured polarographically in saponin‐skinned fibres of RV and VL biopsies of patients and compared with Vmax of healthy VL and myocardium. Effects of increasing concentrations (1–10–100 μM) of Sandimmune®, its vehicle, Cyclosporine (CsA) in ethanol (EtOH), or EtOH alone were tested. The vehicle's effects on MRC complexes were investigated using specific substrates and inhibitors. Ten months after grafting, Vmax of RV and VL of immunosuppressed patients were similar to their Vmax at time of transplantation and to that of control tissues. In Vitro, Sandimmune® significantly decreased Vmax while CsA in EtOH or EtOH exerted small and similar effects. Effects of the vehicle were higher than (RV) or identical to (VL) those of Sandimmune®. The sites of action of the vehicle on MRC were located on complexes I and IV. While unchanged under chronic immunosuppressive therapy, Vmax of RV and VL muscles was depressed by acute exposure to intravenous Sandimmune® in vitro, an effect attributed to its vehicle by inhibition of complexes I and IV of the MRC. This work provides an in vitro proof of a toxic effect on the mitochondria respiratory chain of the vehicle used in the intravenous formulation of Sandimmune® but with no clinical consequences in chronically immunosuppressed patients.
Elucidation of molecular mechanism involved in neuroprotective effect ofCoenzymeQ10 in alcohol‐induced neuropathic pain
Authors: Kandhare Amit D. , Ghosh Pinaki , Ghule Arvindkumar E. , Bodhankar Subhash L. 
Published: September 2012

AbstractThe aim of the present investigation was to evaluate the effect ofCoenzymeQ10 and its combination with vitaminEin alcohol‐induced chronic neuropathic pain. MaleWistar rats were orally treated with alcohol (10 g/kg, 35% v/v, b.i.d.) for 10 weeks. CoenzymeQ10 (25, 50, and 100 mg/kg) and vitamin E (100 mg/kg) were coadministered orally for 1 h after ethanol administration for 10 weeks. Various nerve functions, biochemical, and molecular parameters were assessed. Chronic administration of ethanol for 10 weeks resulted significant development of neuropathic pain. Treatment withCoenzymeQ10 (50 and 100 mg/kg) for 10 weeks showed significant and dose dependently increased in level of nociceptive threshold, endogenous antioxidant, andNa,K‐ATPase enzyme. CoenzymeQ10 (50 and 100 mg/kg) significantly restored the levels of motor nerve conduction velocity and sensory nerve conduction velocity. It also showed significant decrease in levels of endogenous calcium, oxidative–nitrosative stress,TNF‐α,IL‐1β, andIL‐4 level. Alteration in protein expression of polymerase gamma (pol γ) was significantly restored theCoenzymeQ10 treatment. The important finding of the study is that,CoenzymeQ10 (100 mg/kg) and α‐tocopherol (100 mg/kg) combination‐treated rats showed more significant prevention of behavioral, biochemical, and molecular neurotoxic effect of alcohol administration thanCoenzymeQ10 or α‐tocopherol alone treated group. It is evident from the finding of present investigation that plethora of mechanism including inhibition of oxido‐nitrosative stress, release of pro‐inflammatory cytokine, modulation of endogenous biomarker, and protection of pol γ protein expression simultaneously orchestrate to exhibits neuroprotective effect ofCoenzymeQ10, vitamin E and their combination.
Phosphorylation on threonine 11 of β‐dystrobrevin alters its interaction with kinesin heavy chain
Authors: Fratini Federica , Macchia Gianfranco , Torreri Paola , Matteucci Andrea , Salzano Anna Maria , Crescenzi Marco , Macioce Pompeo , Petrucci Tamara C. , Ceccarini Marina 
Published: September 2012

Dystrobrevin family members (α and β) are cytoplasmic components of the dystrophin‐associated glycoprotein complex, a multimeric protein complex first isolated from skeletal muscle, which links the extracellular matrix to the actin cytoskeleton. Dystrobrevin shares high homology with the cysteine‐rich and C‐terminal domains of dystrophin and a common domain organization. The β‐dystrobrevin isoform is restricted to nonmuscle tissues, serves as a scaffold for signaling complexes, and may participate in intracellular transport through its interaction with kinesin heavy chain. We have previously characterized the molecular determinants affecting the β‐dystrobrevin–kinesin heavy chain interaction, among which is cAMP‐dependent protein kinase [protein kinase A (PKA)] phosphorylation of β‐dystrobrevin. In this study, we have identified β‐dystrobrevin residues phosphorylated in vitro by PKA with pull‐down assays, surface plasmon resonance measurements, and MS analysis. Among the identified phosphorylated residues, we demonstrated, by site‐directed mutagenesis, that Thr11 is the regulatory site for the β‐dystrobrevin–kinesin interaction. As dystrobrevin may function as a signaling scaffold for kinases/phosphatases, we also investigated whether β‐dystrobrevin is phosphorylated in vitro by kinases other than PKA. Thr11 was phosphorylated by protein kinase C, suggesting that this represents a key residue modified by the activation of different signaling pathways.Structured digital abstract

Kif5a binds to β‐DB by surface plasmon resonance (View interaction)

β‐DB physically interacts with Kinesin and syntrophin. by pull down (View interaction)
Evaluation of effectiveness of erbium:yttrium–aluminum–garnet laser on atrophic facial acne scars with 22‐MHz digital ultrasonography in a Turkish population
Authors: Engİn Burhan , Kutlubay Zekayi , Karakuş Özge , Yardimci Gürkan , Doğan Zafer , Tüzün Yalçın , Serdaroğlu Server 
Published: September 2012

ABSTRACTScar formation due to acne is a common problem among the young population and significantly affects their quality of life. The aim of this study was to evaluate the efficacy of erbium:yttrium–aluminum–garnet (Er:YAG) laser resurfacing for acne scars and to objectively demonstrate the altering of collagen density in the dermis by 22‐MHz digital ultrasonography. Twenty‐one patients, aged 19–55 years, with facial acne scars were treated with Er:YAG laser. The results of the laser resurfacing were evaluated for the degree of clinical improvement, alteration of the collagen density by 22‐MHz digital ultrasonography and any adverse effects at 3 months. At 3 months after the treatment, good (in 12 patients) and near total (in four patients) clinical improvement was noted in most of the patients compared to baseline. Overall treatment results were 76% (both near total and good) in 16 patients. By ultrasonographic evaluation, the average density of dermal collagen (total density/number of patients) of 21 patients was 32.714 (right cheek) and 32.142 (left cheek) before laser facial resurfacing. At the third month after treatment, the average density of dermal collagen of 21 patients was 36.380 (right cheek) and 38.809 (left cheek). In conclusion, Er:YAG laser skin resurfacing was found to be a safe and effective treatment modality for treatment of atrophic facial acne scars. As public demand grows for less invasive modalities to approach clinical diagnosis and evaluation, digital ultrasonography seems to provide an easy and confidential method for collagen density evaluation.
Dyschromatosis symmetrica hereditaria
Authors: Hayashi Masahiro , Suzuki Tamio 
Published: September 2012

ABSTRACTDyschromatosis symmetrica hereditaria (DSH) is a rare pigmentary genodermatosis, which is acquired by autosomal dominant inheritance with high penetrance. Most cases of this condition have been reported from East Asian countries, including Japan, China and Taiwan. Its symptoms are mixed hyper‐ and hypopigmented macules on the dorsal aspect of the hands and feet and freckle‐like macules on the face. The gene responsible for DSH has been identified as adenosine deaminase acting on RNA1 (ADAR1). The ADAR1 protein catalyzes the transformation of adenosine to inosine in dsRNA substrates (so‐called A‐to‐I editing) and is involved in various activities, such as viral inactivation, structural change of the protein and the resultant cell survival. However, its function in the skin and role in the development of DSH are still unknown. To date, more than 100 mutations of ADAR1 have been reported in patients with DSH, and the catalytic domain deaminase is believed to be crucial to the activities of this gene. Some complications of DSH have been reported and, intriguingly, several patients have been reported to develop neurological symptoms, such as dystonia and mental deterioration. Because ADAR1 plays various important roles in human tissue, we believe that a clarification of the pathogenesis of DSH will promote the understanding of the physiological functions of ADAR1, which will have significant scientific implications.
Two cases of livedo vasculopathy with non‐criteria antiphospholipid antibodies
Authors: Hasegawa Minoru , Fujimoto Manabu , Orito Hidemitsu , Matsushita Takashi , Hamaguchi Yasuhito , Takehara Kazuhiko 
Published: September 2012

ABSTRACTLivedo vasculopathy is characterized by reticular distribution of purpuric macules and papules of the lower legs, caused by intraluminal thrombosis of small vessels. Antiphospholipid antibodies are detected in a subset of these patients. We treated two cases (a 34‐year‐old man and a 46‐year‐old woman) with livedo vasculopathy. In both cases, thrombosis was seen only in the skin. The presence of immunoglobulin (Ig)G or IgM anticardiolipin antibody (Ab), IgG or IgM anti‐β2‐glycoprotein I Ab, or lupus anticoagulant are necessary for criteria‐based diagnosis of antiphospholipid syndrome. However, our patients were negative for these Ab, and instead had either IgG antiphosphatidylethanolamine Ab or IgA anticardiolipin Ab. These Ab are suggestive of antiphospholipid syndrome but are not considered “criteria” Ab. This report demonstrates the existence of antiphosphatidylethanolamine Ab or IgA anticardiolipin Ab in patients with livedo vasculopathy. However, the frequency and significance of these Ab in livedo vasculopathy should be confirmed in larger longitudinal studies.
Missed and inconsistent classification of current drinkers: results from the 2005 US National Alcohol Survey
Authors: Midanik Lorraine T. , Ye Yu , Greenfield Thomas K. , Kerr William 
Published: September 2012

AbstractAimsThis study compares current 12‐month drinkers who do not report drinking in the last 30 days with current drinkers who drank in the last 30 days and assesses possible misclassification errors from use of a 30‐day consumption measure.DesignData are from the 2005 US National Alcohol Survey (n = 6919), a national household probability survey.SettingTelephone interviews were used to measure alcohol use and alcohol‐related problems.ParticipantsThis study compared 1300 current drinkers who did not drink in the last 30 days with 2956 current drinkers who drank in the last 30 days.MeasurementsVolume was measured by quantity/frequency scales (12‐month and 30‐day) and a graduated frequency scale (12‐month). Both groups were compared by demographic, alcohol volume, days of five or more drinks, social consequences and dependence measures.FindingsResults indicate a significantly lower prevalence rate of current drinking for 30‐day measures—47.3% (45.8%, 48.8%) versus 67.3% (66.0%, 68.7%) with 12‐month measures. Further, 385 non‐30‐day drinkers reported 12‐month drinking frequencies of once a month or more often, suggesting possible inconsistent reporting of their alcohol use. When this group of ‘inconsistent’ respondents is compared with the 915 non‐30‐day current drinkers who reported less than monthly drinking, they reported significantly higher yearly volume, days of five or more drinks, mean social consequences and proportion reporting alcohol dependence.ConclusionsIn population surveys assessing alcohol use, asking about the previous 12 months rather than the past 30 days provides higher estimates of current use, including more days of heavy episodic use.
Vitamin C deficiency accelerates bone loss inducing an increase in PPAR‐γ expression in SMP30 knockout mice
Authors: Park Jin‐Kyu , Lee Eun‐Mi , Kim Ah‐Young , Lee Eun‐Joo , Min Chang‐Woo , Kang Kyung‐Ku , Lee Myeong‐Mi , Jeong Kyu‐Shik 
Published: September 2012

SummarySenescence marker protein (SMP) 30 knockout (KO) mice display symptoms of scurvy, including spontaneous bone fractures, and this was considered to be induced by a failure of collagen synthesis owing to vitamin C deficiency. However, low bone mineral density is also known to be associated with spontaneous bone fracture. Therefore, we investigated the effects of vitamin C deficiency on the balance between osteoblasts and osteoclasts in SMP30 KO mice as evidenced by histopathology. All mice were fed a vitamin C‐free diet, and only one group (KV) mice were given water containing 1.5 g/l of vitamin C, whereas wild‐type (WT) and KO mice were given normal drinking tap water without vitamin C for 16 weeks. After 16 weeks, all femur samples were removed for histopathological examination. The femurs of KO mice showed significantly reduced bone area and decreased number of osteoblasts compared with those of WT mice and KV mice. KO mice also exhibited the lowest level of alkaline phosphatase (ALP) expression in their femurs. However, KO mice showed the most elevated expression of the receptor activator of nuclear factor kappa‐B ligand (RANKL). Moreover, KO mice had the strongest peroxisome proliferator‐activated receptor (PPAR)‐γ expression level in their osteoblasts and the highest number of TUNEL‐positive bone marrow cells. Therefore, we concluded that vitamin C deficiency plays an important role in spontaneous bone fracture by inhibiting osteoblast differentiation and promoting transition of osteoblasts to adipocytes, and this could in turn be related to the increased PPAR‐γ expression.
MMP7 is a target of the tumour‐associated antigen EpCAM
Authors: Denzel Sabine , Mack Brigitte , Eggert Carola , Massoner Petra , Stöcklein Nikolas , Kemming Dirk , Harréus Ulrich , Gires Olivier 
Published: September 2012

SummaryEpithelial cell adhesion molecule (EpCAM) is a single‐transmembrane protein, which is involved in numerous cellular processes including cell adhesion, proliferation, maintenance of stemness of embryonic cells and progenitors, migration and invasion. Activation of signal transduction by EpCAM is warranted by regulated intramembrane proteolysis and nuclear translocation of the intracellular domain EpICD. Here, we describe matrix metalloproteinase 7 (MMP7) as a target gene of EpCAM signalling viaEpICD nuclear translocation. EpCAM and MMP7 expression pattern and levels positively correlated in vitro and in vivo, and were strongly elevated in primary carcinomas of the head and neck area. Hence, MMP7 is a novel target of EpCAM signalling.
Effect of cypermethrin, carbendazim and their combination on male albino rat serum
Authors: Veerappan Muthuviveganandavel , Hwang InHo , Pandurangan Muthuraman 
Published: September 2012

SummaryAlpha‐cypermethrin and carbendazim are synthetic; α‐cypermethrin belongs to a class of synthetic pyrethroids and carbendazim belongs to the class of carbamate fungicides. The current study was carried out to evaluate the low‐dose exposure of individual and mixed forms of cypermethrin and carbendazim. α‐cypermethrin was used at 0.06, 0.12, 0.30 and 0.60 mg/kg body weight (bw), carbendazim was at 0.48, 0.96, 2.4 and 4.8 mg/kg bw and combined doses (cypermethrin: 0.06, 0.12, 0.30 and 0.60 mg/kg.bwt + carbendazim: 0.48, 0.96, 2.4 and 4.8 mg/kg.bwt) for 12 h and 24 h. The biochemical parameters and serum markey enzymes were analysed. The biochemical parameters include serum total protein, glucose, cholesterol, urea, uric acid, calcium, phosphorous, albumin and creatinine and serum marker enzymes such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), γ‐glutamyl transpeptidase (GGT) and amylase were ascertained. Results indicated simultaneous changes in serum marker enzyme activity (ALT, AST, ALP, GGT and amylase) and biochemical markers (*P < 0.05, **P < 0.01 and ***P < 0.001). The experimental results indicate that even low‐dose use of the synthetic pyrethroid carbamate and their combined form results in consequential negative effects on cell function.
Keratins in colorectal epithelial function and disease
Authors: Majumdar Debabrata , Tiernan James P. , Lobo Alan J. , Evans Caroline A. , Corfe Bernard M. 
Published: September 2012

SummaryKeratins are the largest subgroup of intermediate filament proteins, which are an important constituent of the cellular cytoskeleton. The principally expressed keratins (K) of the intestinal epithelium are K8, K18 and K19. The specific keratin profile of a particular epithelium provides it with strength and integrity. In the colon, keratins have been shown to regulate electrolyte transport, likely by targeting ion transporters to their correct location in the colonocytes.Keratins are highly dynamic and are subject to post‐translational modifications including phosphorylation, acetylation and glycosylation. These affect the filament dynamics and hence solubility of keratins and may contribute to protection against degradation. Keratin null mice (K8−/−) develop colitis, and abnormal keratin mutations have been shown to be associated with inflammatory bowel disease (IBD). Abnormal expression of K7 and K20 has been noted in colitis‐associated dysplasia and cancers. In sporadic colorectal cancers (CRCs) may be useful in predicting tumour prognosis; a low K20 expression is noted in CRCs with high microsatellite instability; and keratins have been noted as dysregulated in peri‐adenomatous fields. Caspase‐cleaved fragment of K18 (M30) in the serum of patients with CRC has been used as a marker of cancer load and to assess response to therapy. These data suggest an emerging importance of keratins in maintaining normal function of the gastrointestinal epithelium as well as being a marker of various colorectal diseases. This review will primarily focus on the biology of these proteins, physiological functions and alterations in IBD and CRCs.