bluuebunny/crossref_metadata_2025_split huggingface dataset:
Review of the history, status and prospects of the black tiger shrimp (Penaeus monodon) hatchery sector in Bangladesh
Authors: Debnath Partho , Khan Shamsul H. , Karim Manjurul , Belton Ben , Mohan Chadag Vishnumurthy , Phillips Michael 
Published: March 2015

AbstractCulture of shrimp, mainly Penaeus monodon, makes an important contribution to Bangladesh's aquaculture industry. Shrimp hatcheries are the foundation on which the shrimp industry is built. Hatchery performance and management therefore has extremely important implications for the long‐term viability of the entire shrimp sector, especially given its vulnerability to diseases such as white spot syndrome virus (WSSV). This article provides a review of the history of shrimp hatchery development in Bangladesh and an overview of the current status of the shrimp hatchery sector and the challenges it faces. This article also provides a unique historical analysis of shrimp broodstock performance and rates of WSSV infection using data collected from hatcheries between 2007 and 2013. This reveals sharp declines in broodstock quality and performance over time, and high rates of WSSV infection, with potential major implications for the future sustainability of the sector.
Mozambique Metical Exchange Rate Dynamics: Evidence of Fractional Co‐Integration in the USA and South African Rates
Authors: Barros Carlos P. , Gil‐Alana Luis Alberiko , Faria João 
Published: March 2015

AbstractThis paper studies the exchange rate dynamics of the Mozambique metical with respect to the US dollar and the South African rand. However, instead of using standard I(0)/I(1) techniques, we use long memory and fractionally integrated and co‐integrated models. Our results indicate that the two exchange rates are highly persistent, with orders of integration equal to or above 1. They also seem to be co‐integrated, with an order of integration close to albeit above 0 but with an AR coefficient very close to 1. Thus, although the two series seem to be fractionally co‐integrated, shocks in the long‐run relationship between the two variables are persistent and take a long time to disappear.
Perinatal management of neonatal alloimmune thrombocytopenia associated with anti‐group A antibody
Authors: Ueda H. , Sugiura T. , Katano K. , Matsuhashi M. , Kato S. , Ito K. , Nagasaki R. , Kato T. , Tsuno N. H. , Saitoh S. 
Published: March 2015

SUMMARYObjectiveTo prevent neonatal alloimmune thrombocytopenia due to anti‐group A antibody perinatal management was performed.BackgroundWe previously reported a case of severe intracranial haemorrhage associated with neonatal alloimmune thrombocytopenia due to anti‐group A isoantibody.Material/MethodsA 40‐year‐old Japanese woman, gravida 4 para 1, was pregnant with her second baby. The previous sibling developed severe thrombocytopenia and died 10 days after birth due to intracranial haemorrhage. He was diagnosed with neonatal alloimmune thrombocytopenia; the causative antibody was found to be the anti‐group A antibody. Prednisone was started at 7 weeks' gestational age. Intravenous immunoglobulin 1 g kg−1 week−1 was started at 29 weeks' gestational age and continued to delivery. Serological studies and genotyping were performed.ResultsThe second boy was delivered at 33 weeks' gestational age by caesarean section. He was discharged without intracranial haemorrhage or thrombocytopenia. The anti‐group A antibody titre in the maternal serum was 2048–4096 (normal range: 4–64). The anti‐group A antibody titre in the newborn's serum was 4. Cross‐matching between the maternal serum and the paternal platelets was positive.ConclusionOwing to the history of neonatal alloimmune thrombocytopenia causing intracranial haemorrhage and death of the previous sibling, strict follow‐up of the subsequent pregnancy was conducted.
Ethylene mediates brassinosteroid‐induced stomatal closure via Gα protein‐activated hydrogen peroxide and nitric oxide production in Arabidopsis
Authors: Shi Chenyu , Qi Cheng , Ren Hongyan , Huang Aixia , Hei Shumei , She Xiaoping 
Published: March 2015

SummaryBrassinosteroids (BRs) are essential for plant growth and development; however, whether and how they promote stomatal closure is not fully clear. In this study, we report that 24‐epibrassinolide (EBR), a bioactive BR, induces stomatal closure in Arabidopsis (Arabidopsis thaliana) by triggering a signal transduction pathway including ethylene synthesis, the activation of Gα protein, and hydrogen peroxide (H2O2) and nitric oxide (NO) production. EBR initiated a marked rise in ethylene, H2O2 and NO levels, necessary for stomatal closure in the wild type. These effects were abolished in mutant bri1‐301, and EBR failed to close the stomata of gpa1 mutants. Next, we found that both ethylene and Gα mediate the inductive effects of EBR on H2O2 and NO production. EBR‐triggered H2O2 and NO accumulation were canceled in the etr1 and gpa1 mutants, but were strengthened in the eto1‐1 mutant and the cGα line (constitutively overexpressing the G protein α‐subunit AtGPA1). Exogenously applied H2O2 or sodium nitroprusside (SNP) rescued the defects of etr1‐3 and gpa1 or etr1 and gpa1 mutants in EBR‐induced stomatal closure, whereas the stomata of eto1‐1/AtrbohF and cGα/AtrbohF or eto1‐1/nia1‐2 and cGα/nia1‐2 constructs had an analogous response to H2O2 or SNP as those of AtrbohF or Nia1‐2 mutants. Moreover, we provided evidence that Gα plays an important role in the responses of guard cells to ethylene. Gα activator CTX largely restored the lesion of the etr1‐3 mutant, but ethylene precursor ACC failed to rescue the defects of gpa1 mutants in EBR‐induced stomatal closure. Lastly, we demonstrated that Gα‐activated H2O2 production is required for NO synthesis. EBR failed to induce NO synthesis in mutant AtrbohF, but it led to H2O2 production in mutant Nia1‐2. Exogenously applied SNP rescued the defect of AtrbohF in EBR‐induced stomatal closure, but H2O2 did not reverse the lesion of EBR‐induced stomatal closure in Nia1‐2. Together, our results strongly suggest a signaling pathway in which EBR induces ethylene synthesis, thereby activating Gα, and then promotes AtrbohF‐dependent H2O2 production and subsequent Nia1‐catalyzed NO accumulation, and finally closes stomata.
PRC1 is taking the lead in PcG repression
Authors: Merini Wiam , Calonje Myriam 
Published: March 2015

SummaryPolycomb group (PcG) proteins constitute a major epigenetic mechanism for gene repression throughout the plant life. For a long time, the PcG mechanism has been proposed to follow a hierarchical recruitment of PcG repressive complexes (PRCs) to target genes in which the binding of PRC2 and the incorporation of H3 lysine 27 trimethyl marks led to recruitment of PRC1, which in turn mediated H2A monoubiquitination. However, recent studies have turned this model upside‐down by showing that PRC1 activity can be required for PRC2 recruitment and H3K27me3 marking. Here, we review the current knowledge on plant PRC1 composition and mechanisms of repression, as well as its role during plant development.
Tolerance of platelet concentrates treated with UVC‐light only for pathogen reduction – a phase I clinical trial
Authors: Thiele T. , Pohler P. , Kohlmann T. , Sümnig A. , Aurich K. , Selleng K. , Westphal A. , Bakchoul T. , Petersmann A. , Müller T. H. , Greinacher A. , Seltsam A. 
Published: March 2015

BackgroundThe THERAFLEX UV‐Platelets pathogen reduction system for platelet concentrates (PCs) operates with ultraviolet C light (UVC; 254 nm) only without addition of photosensitizers. This phase I study evaluated safety and tolerability of autologous UVC‐irradiated PCs in healthy volunteers.MethodsEleven volunteers underwent two single (series 1 and 2) and one double apheresis (series 3). PCs were treated with UVC, stored for 48 h and retransfused in a dose‐escalation scheme: 12·5, 25% and 50% of a PC (series 1); one complete PC (series 2); two PCs (series 3). Platelet counts, fibrinogen, activated partial thromboplastin time, prothrombin time, D‐dimer, standard haematology, temperature, heart rate, blood pressure and clinical chemistry parameters were measured. One‐ and 24‐h corrected count increments were determined in series 2 and 3. Platelet‐specific antibodies were assessed before and at the end of the study.ResultsNeither adverse reactions related to transfusions nor antibodies against UVC‐treated platelets were observed. Corrected count increments did not differ between series 2 and 3.ConclusionsRepeated transfusions of autologous UVC‐treated PCs were well tolerated and did not induce antibody responses in all volunteers studied. EudraCT No. 2010‐023404‐26.
Draft Genome Sequences of Acinetobacter harbinensis Strain HITLi 7
T
, Isolated from River Water
Authors: Zhang Duoying , Li Weiguang , Qin Wen , Huang Xiaofei , Gong Xujin 
Published: March 2015

ABSTRACT

Acinetobacter harbinensis
HITLi strain 7
T
, isolated from river water, has the ability to remove ammonium and organic chemicals at 2°C. The genome sequences might be useful for investigating the low-temperature adaptability and nitrogen or organic chemical metabolism.
Whole-Genome Sequences of Chlamydia psittaci Strain HJ, Isolated from Meat Pigeons with Severe Respiratory Distress and High Mortality
Authors: Zhang Qiang , Wu Zongxue , Sun Ruixue , Chu Jun , Han Er , Zhang Yinxin , Ling Yong , Gong Yanping , Li Dongfang , Wu Honglong , He Cheng , Ni Peixiang 
Published: March 2015

ABSTRACT

The obligate intracellular Gram-negative bacterium
Chlamydia psittaci
causes systemic disease in psittacine birds, domestic poultry, and wild fowl. Importantly,
C. psittaci
may cause pneumonia, encephalitis, endocarditis, and even death in humans. The potential of pigeons as a source of human psittacosis is supported worldwide by relatively high seroconversion rates in the birds. This study reports the whole-genome sequencing of
C. psittaci
strain HJ, isolated from meat pigeons suffering from severe pneumonia and high mortality in 2013 in Hebei, China.
Whole-Genome Analysis of Quorum-Sensing Burkholderia sp. Strain A9
Authors: Chan Kok-Gan , Chen Jian Woon , Tee Kok Keng , Chang Chien-Yi , Yin Wai-Fong , Chan Xin-Yue 
Published: March 2015

ABSTRACT

Burkholderia spp. rely on
N
-acyl homoserine lactone as quorum-sensing signal molecules which coordinate their phenotype at the population level. In this work, we present the whole genome of Burkholderia sp. strain A9, which enables the discovery of its
N
-acyl homoserine lactone synthase gene.
Draft Genome Sequence of “Candidatus Methylacidiphilum kamchatkense” Strain Kam1, a Thermoacidophilic Methanotrophic Verrucomicrobium
Authors: Erikstad Helge-André , Birkeland Nils-Kåre 
Published: March 2015

ABSTRACT

“Candidatus Methylacidiphilum kamchatkense” strain Kam1 is an aerobic methane-oxidizing thermoacidophilic bacterium belonging to the
Verrucomicrobia
phylum. It was recovered from an acidic geothermal site in Uzon Caldera, Kamchatka, Russian Federation. Its genome possesses three complete
pmoCAB
gene clusters encoding particulate methane monooxygenase enzymes and a complete Calvin-Benson-Bassham cycle for carbon assimilation.
Genome Sequences of Serotype A6 Mannheimia haemolytica Isolates D174 and D38 Recovered from Bovine Pneumonia
Authors: Hauglund Melissa J. , Tatum Fred M. , Bayles Darrell O. , Maheswaran Samuel K. , Briggs Robert E. 
Published: March 2015

ABSTRACTHere, we report two genomes, one complete and one draft, from virulent bovine strains ofMannheimia haemolyticaserotype A6 recovered prior to the field usage of modern antimicrobial drugs.
Draft Genome Sequence of Methyloferula stellata AR4, an Obligate Methanotroph Possessing Only a Soluble Methane Monooxygenase
Authors: Dedysh Svetlana N. , Naumoff Daniil G. , Vorobev Alexey V. , Kyrpides Nikos , Woyke Tanja , Shapiro Nicole , Crombie Andrew T. , Murrell J. Colin , Kalyuzhnaya Marina G. , Smirnova Angela V. , Dunfield Peter F. 
Published: March 2015

ABSTRACT

Methyloferula stellata
AR4 is an aerobic acidophilic methanotroph, which, in contrast to most known methanotrophs but similar to Methylocella spp., possesses only a soluble methane monooxygenase. However, it differs from Methylocella spp. by its inability to grow on multicarbon substrates. Here, we report the draft genome sequence of this bacterium.
Out-of-hours palliative care: what are the educational needs and preferences of general practitioners?
Authors: Magee Claire , Koffman Jonathan 
Published: March 2015

ObjectivesOffering genuine choice to patients over place of care and death requires the provision of high-quality palliative care 24/7. This study examines the confidence of out-of-hours general practitioners (GPs) in symptom control and end of life prescribing, and identifies their educational needs and preferences in order to inform recommendations for future education.MethodsA self-completion postal survey was designed and sent to 1005 GPs working for an independent provider of out-of-hours services across England. Quantitative data were analysed using descriptive statistics and non-parametric tests.Results203 (20.3%) GPs completed the survey questionnaire; 13.3% (n=27) worked exclusively out-of-hours. Confidence in assessing palliative care emergencies (42.8%, n=87: ‘not so confident’ or ‘not at all confident’), managing symptoms in non-cancer patients (39.4%, n=80) and prescribing a new syringe driver (39.0%, n=79) was lowest. Lower confidence was associated with infrequent exposure to palliative patients (p<0.05) and lack of training in palliative care (p<0.05); 12.8% (n=26) had never received formal training. Educational preferences were closely associated with confidence (p<0.0005); the topics above were most requested. E-learning was the preferred method (67.5%, n=137). 82.1% (n=165) believed training focused on out-of-hours work would be beneficial.ConclusionsWe identify that confidence in key palliative care competences is severely lacking. Educational strategies to address this concern must be targeted at GPs preferences for content and mode of delivery. Regular e-learning is favoured, but should be blended with other approaches that promote engagement including out-of-hours themed workshops and case discussion. Specialist palliative care services should engage with out-of-hours providers to support education.
Assessing the internal consistency and temporal stability of advance directives generated by an interactive, online computer program
Authors: Schubart Jane R , Camacho Fabian , Green Michael J , Rush Kimberly A , Levi Benjamin H 
Published: March 2015

ObjectiveEvaluate the internal consistency and temporal stability of advance directives (ADs) generated by an interactive, online computer program.Methods33 participants completed the program at three visits, 2 weeks apart. Agreement rates were calculated for theGeneral Wishescomponent of the AD. The test–retest method was used to examine the temporal stability of theSpecific Wish for Treatmentcomponent which contains five clinical scenarios.ResultsGeneral Wishesremained stable with 94% selecting the identical response at each visit. For theSpecific Wish for Treatmentscale,significant variations in test–retest correlations existed (ie, ρ=0.32–0.78, between time points 1 and 2); however within scenario, correlations did not significantly vary between time points. Temporal stability was lower in theSpecific Wish for Treatmentscale compared withGeneral Wishes (average ρ=0.59, between time points 1 and 2; and ρ=0.75, between time points 2 and 3).ConclusionsADs generated by an online decision aid demonstrate good temporal stability, with highest stability forGeneral Wishesand moderate stability forSpecific Wish for Treatmentregarding medical treatments in specific clinical scenarios. Internal consistency for wish for treatment across all time points and scenarios was high (Cronbach α>0.90).
Lung Tumor Suppressor GPRC5A Binds EGFR and Restrains Its Effector Signaling
Authors: Zhong Shuangshuang , Yin Huijing , Liao Yueling , Yao Feng , Li Qi , Zhang Jie , Jiao Huike , Zhao Yongxu , Xu Dongliang , Liu Shuli , Song Hongyong , Gao Yong , Liu Jingyi , Ma Lina , Pang Zhi , Yang Ruixu , Ding Chengyi , Sun Beibei , Lin Xiaofeng , Ye Xiaofeng , Guo Wenzheng , Han Baohui , Zhou Binhua P. , Chin Y. Eugene , Deng Jiong 
Published: March 2015

Abstract
GPRC5A is a G-protein–coupled receptor expressed in lung tissue but repressed in most human lung cancers. Studies in Gprc5a−/− mice have established its role as a tumor-suppressor function in this setting, but the basis for its role has been obscure. Here, we report that GPRC5A functions as a negative modulator of EGFR signaling. Mouse tracheal epithelial cells (MTEC) from Gprc5a−/− mice exhibited a relative increase in EGFR and downstream STAT3 signaling, whereas GPRC5A expression inhibited EGFR and STAT3 signaling. GPRC5A physically interacted with EGFR through its transmembrane domain, which was required for its EGFR inhibitory activity. Gprc5a−/− MTEC were much more susceptible to EGFR inhibitors than wild-type MTEC, suggesting their dependence on EGFR signaling for proliferation and survival. Dysregulated EGFR and STAT3 were identified in the normal epithelia of small and terminal bronchioles as well as tumors of Gprc5a−/− mouse lungs. Moreover, in these lungs EGFR inhibitor treatment inhibited EGFR and STAT3 activation along with cell proliferation. Finally, overexpression of ectopic GPRC5A in human non–small cell lung carcinoma cells inhibited both EGF-induced and constitutively activated EGFR signaling. Taken together, our results show how GPRC5A deficiency leads to dysregulated EGFR and STAT3 signaling and lung tumorigenesis. Cancer Res; 75(9); 1801–14. ©2015 AACR.
Molecular Portraits of Epithelial, Mesenchymal, and Hybrid States in Lung Adenocarcinoma and Their Relevance to Survival
Authors: Schliekelman Mark J. , Taguchi Ayumu , Zhu Jun , Dai Xudong , Rodriguez Jaime , Celiktas Muge , Zhang Qing , Chin Alice , Wong Chee-Hong , Wang Hong , McFerrin Lisa , Selamat Suhaida A. , Yang Chenchen , Kroh Evan M. , Garg Kavita S. , Behrens Carmen , Gazdar Adi F. , Laird-Offringa Ite A. , Tewari Muneesh , Wistuba Ignacio I. , Thiery Jean P. , Hanash Samir M. 
Published: March 2015

Abstract
Epithelial-to-mesenchymal transition (EMT) is a key process associated with tumor progression and metastasis. To define molecular features associated with EMT states, we undertook an integrative approach combining mRNA, miRNA, DNA methylation, and proteomic profiles of 38 cell populations representative of the genomic heterogeneity in lung adenocarcinoma. The resulting data were integrated with functional profiles consisting of cell invasiveness, adhesion, and motility. A subset of cell lines that were readily defined as epithelial or mesenchymal based on their morphology and E-cadherin and vimentin expression elicited distinctive molecular signatures. Other cell populations displayed intermediate/hybrid states of EMT, with mixed epithelial and mesenchymal characteristics. A dominant proteomic feature of aggressive hybrid cell lines was upregulation of cytoskeletal and actin-binding proteins, a signature shared with mesenchymal cell lines. Cytoskeletal reorganization preceded loss of E-cadherin in epithelial cells in which EMT was induced by TGFβ. A set of transcripts corresponding to the mesenchymal protein signature enriched in cytoskeletal proteins was found to be predictive of survival in independent datasets of lung adenocarcinomas. Our findings point to an association between cytoskeletal and actin-binding proteins, a mesenchymal or hybrid EMT phenotype and invasive properties of lung adenocarcinomas. Cancer Res; 75(9); 1789–800. ©2015 AACR.
Expression of Hedgehog Pathway Mediator GLI Represents a Negative Prognostic Marker in Human Acute Myeloid Leukemia and Its Inhibition Exerts Antileukemic Effects
Authors: Wellbrock Jasmin , Latuske Emily , Köhler Julian , Wagner Katharina , Stamm Hauke , Vettorazzi Eik , Vohwinkel Gabi , Klokow Marianne , Uibeleisen Roswitha , Ehm Patrick , Riecken Kristoffer , Loges Sonja , Thol Felicitas , Schubert Claudia , Amling Michael , Jücker Manfred , Bokemeyer Carsten , Heuser Michael , Krauter Jürgen , Fiedler Walter 
Published: March 2015

Abstract
Purpose: The Hedgehog pathway plays an important role in stem-cell biology and malignant transformation. Therefore, we investigated the expression and prognostic impact of Hedgehog pathway members in acute myeloid leukemia (AML).
Experimental Design: Pretreatment samples from 104 newly diagnosed AML patients (AMLSG 07-04 trial) were analyzed by qPCR, and expression of Hedgehog family members was correlated with clinical outcome. Inhibition of GLI by GANT61 or shRNA was investigated in AML cells in vitro and in vivo.
Results: Expression of receptors Smoothened and Patched-1 and their downstream mediators, GLI1, GLI2, and GLI3, was found in AML patients in contrast to Hedgehog ligands. GLI2 expression had a significant negative influence on event-free survival (EFS), relapse-free survival (RFS), and overall survival (OS; P = 0.037, 0.026, and 0.013, respectively) and was correlated with FLT3 mutational status (P &lt; 0.001). Analysis of a second, independent patient cohort confirmed the negative impact of GLI2 on EFS and OS (P = 0.007 and 0.003, respectively; n = 290). Within this cohort, GLI1 had a negative prognostic impact (P &lt; 0.001 for both EFS and OS). Although AML cells did not express Hedgehog ligands by qPCR, AML patients had significantly increased Desert Hedgehog (DHH) plasma levels compared with healthy subjects (P = 0.002), in whom DHH was presumably provided by bone marrow niche cells. Moreover, the GLI inhibitor GANT61 or knockdown of GLI1/2 by shRNA caused antileukemic effects, including induction of apoptosis, reduced proliferation, and colony formation in AML cells, and a survival benefit in mice.
Conclusions: GLI expression is a negative prognostic factor and might represent a novel druggable target in AML. Clin Cancer Res; 21(10); 2388–98. ©2015 AACR.
Mad Maria, Intermedialidades e Memória dos Sentidos: a presentificação do passado em minisséries históricas
Authors: Wajnman Solange 
Published: March 2015

<p>O artigo investiga as maneiras pelas quais os objetos em <em>Mad Maria</em> - minissérie veiculada pela Rede Globo - são construídos de modo a criar uma memória sensorial. Identifica na linguagem audiovisual dos objetos de cenário e figurino o emprego de técnicas de presentificação do passado que atualizam aspectos da memória nacional e possibilitam uma experiência no âmbito da recepção. A partir da teoria da materialidade e da intermedialidade buscamos captar as práticas híbridas da cultura digital</p>