bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spillover Effects in Health Service Use: Evidence From Mental Health Care Using First‐Year College Housing Assignments
Authors: Golberstein Ezra , Eisenberg Daniel , Downs Marilyn F. 
Published: November 2014

AbstractSpillover effects in health service use may represent an important externality of individual treatment decisions and are important for understanding the consequences of interventions to improve access to health care. This study is the first to our knowledge to examine causal spillover effects for mental health service use. We exploit the natural experiment of first‐year student housing assignments at two universities using survey data that we collected. When the peer group is defined at the roommate level, we do not find any spillover effects on service use. When the peer group is defined at the hall level, we find positive spillover effects—peers' service use increases one's own service use—and this effect is driven by individuals with prior experience with mental health services. We also find some evidence that the mechanism behind this effect is improved beliefs about treatment effectiveness. Copyright © 2014 John Wiley & Sons, Ltd.
Dye distance mapping using waveguide evanescent field fluorescence microscopy and its application to cell biology
Authors: Fleissner Frederik , Morawitz Michael , Dixon S. Jeffrey , Langbein Uwe , Mittler Silvia 
Published: November 2014

Previous studies have measured the distance between cells and the substratum at sites of adhesion via the emission of a fluorescent dye and waveguide methods. Here, we demonstrate a novel approach to measure the position of fluorescent dyes above a waveguide surface in the 10–200 nm distance range throughout an entire area, yielding a 2D dye distance map or a 3D contour plot. The dye is located in a multilayered Langmuir Blodgett (LB) film or in the plasma membrane of a cell. Waveguide evanescent field fluorescence (WEFF) images obtained using two different waveguide modes are employed allowing, with a simple mathematical approach, the calculation of dye distance maps. Ultra‐thin steps made using LB technology, adhesion distances and the bending of the plasma membrane between focal adhesions of osteoblastic cells are shown as examples. The errors are discussed.
False color representation of a dye distance map with four osteoblasts. The inset represents an overexposed WEFF image of the same field of view.magnified imageFalse color representation of a dye distance map with four osteoblasts. The inset represents an overexposed WEFF image of the same field of view.
Detection and controlled depletion of cancer cells using photothermal phase microscopy
Authors: Turko Nir Abraham , Barnea Itay , Blum Omry , Korenstein Rafi , Shaked Natan Tzvi 
Published: November 2014

We present a dual‐modality technique based on wide‐field photothermal (PT) interferometric phase imaging and simultaneous PT ablation to selectively deplete specific cell populations labelled by plasmonic nanoparticles. This combined technique utilizes the plasmonic reaction of gold nanoparticles under optical excitation to produce PT imaging contrast by inducing local phase changes when the excitation power is weak, or ablation of selected cells when increasing the excitation power. Controlling the entire process is carried out by dynamic quantitative phase imaging of all cells (labelled and unlabelled). We demonstrate our ability to detect and specifically ablate in vitro cancer cells over‐expressing epidermal growth factor receptors (EGFRs), labelled with plasmonic nanoparticles, in the presence of either EGFR under‐expressing cancer cells or white blood cells. The latter demonstration establishes an initial model for depletion of circulating tumour cells in blood. The proposed system is able to image in wide field the label‐free quantitative phase profile together with the PT phase profile of the sample, and provides the ability of both detection and selective cell ablation in a controlled environment.
Quantitative phase imaging with molecular specificity and specific cell depletion. (a) Label‐free quantitative phase profiles of mixed population of EGFR+/EGFR– cancer cells. (b) When weak modulated PT excitation is applied, selective phase contrast is generated in the modulation frequency only for the EGFR+ cancer cells labelled with plasmonic nanoparticles. (c) When stronger modulated PT excitation is applied, selective ablation of the EGFR+ cancer cells labelled with plasmonic nanoparticles occurs. White scalebars represent 10 µm upon sample.magnified imageQuantitative phase imaging with molecular specificity and specific cell depletion. (a) Label‐free quantitative phase profiles of mixed population of EGFR+/EGFR– cancer cells. (b) When weak modulated PT excitation is applied, selective phase contrast is generated in the modulation frequency only for the EGFR+ cancer cells labelled with plasmonic nanoparticles. (c) When stronger modulated PT excitation is applied, selective ablation of the EGFR+ cancer cells labelled with plasmonic nanoparticles occurs. White scalebars represent 10 µm upon sample.
Multifunctional spider silk polymers for gene delivery to human mesenchymal stem cells
Authors: Tokareva Olena S. , Glettig Dean L. , Abbott Rosalyn D. , Kaplan David L. 
Published: November 2014

AbstractNon‐viral gene delivery systems are important transport vehicles that can be safe and effective alternatives to currently available viral systems. A new family of multifunctional spider silk‐based gene carriers was bioengineered and found capable of targeting human mesenchymal stem cells (hMSCs). These carriers successfully delivered DNA to the nucleus of these mammalian cells. The presence of specific functional sequences in the recombinant proteins, such as a nuclear localization sequence (NLS) of the large tumor (T) antigen of the Simian virus 40 (SV40), an hMSC high affinity binding peptide (HAB), and a translocation motif (TLM) of the hepatitis‐B virus surface protein (PreS2), and their roles in mitigation and enhancement of gene transfection efficiency towards hMSCs were characterized. The results demonstrate that these bioengineered spider silk proteins serve as effective carriers, without the well‐known complications associated with viral delivery systems. © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 103B: 1390–1401, 2015.
TSH Elevations as the First Laboratory Evidence for Pseudohypoparathyroidism Type Ib (PHP-Ib)
Authors: Molinaro Angelo , Tiosano Dov , Takatani Rieko , Chrysis Dionisios , Russell William , Koscielniak Nikolas , Kottler Marie-Laure , Agretti Patrizia , De Marco Giuseppina , Ahtiainen Petteri , Christov Marta , Mäkitie Outi , Tonacchera Massimo , Jüppner Harald 
Published: November 2014

ABSTRACT

Hypocalcemia and hyperphosphatemia because of resistance toward parathyroid hormone (PTH) in the proximal renal tubules are the most prominent abnormalities in patients affected by pseudohypoparathyroidism type Ib (PHP-Ib). In this rare disorder, which is caused by GNAS methylation changes, resistance can occur toward other hormones, such as thyroid-stimulating hormone (TSH), that mediate their actions through G protein-coupled receptors. However, these additional laboratory abnormalities are usually not recognized until PTH-resistant hypocalcemia becomes clinically apparent. We now describe four pediatric patients, first diagnosed with subclinical or overt hypothyroidism between the ages of 0.2 and 15 years, who developed overt PTH-resistance 3 to 20 years later. Although anti-thyroperoxidase (anti-TPO) antibodies provided a plausible explanation for hypothyroidism in one of these patients, this and two other patients revealed broad epigenetic GNAS abnormalities, which included loss of methylation (LOM) at exons AS, XL, and A/B, and gain of methylation at exon NESP55; ie, findings consistent with PHP-Ib. LOM at GNAS exon A/B alone led in the fourth patient to the identification of a maternally inherited 3-kb STX16 deletion, a well-established cause of autosomal dominant PHP-Ib. Although GNAS methylation changes were not detected in additional pediatric and adult patients with subclinical hypothyroidism (23 pediatric and 39 adult cases), hypothyroidism can obviously be the initial finding in PHP-Ib patients. One should therefore consider measuring PTH, along with calcium and phosphate, in patients with unexplained hypothyroidism for extended periods of time to avoid hypocalcemia and associated clinical complications. © 2014 American Society for Bone and Mineral Research © 2014 American Society for Bone and Mineral Research.
ERK1 and ERK2 Regulate Chondrocyte Terminal Differentiation During Endochondral Bone Formation
Authors: Chen Zhijun , Yue Susan X , Zhou Guang , Greenfield Edward M , Murakami Shunichi 
Published: November 2014

ABSTRACT

Chondrocytes in the epiphyseal cartilage undergo terminal differentiation prior to their removal through apoptosis. To examine the role of ERK1 and ERK2 in chondrocyte terminal differentiation, we generated Osterix (Osx)-Cre; ERK1–/–; ERK2flox/flox mice (conditional knockout Osx [cKOosx]), in which ERK1 and ERK2 were deleted in hypertrophic chondrocytes. These cKOosx mice were grossly normal in size at birth, but by 3 weeks of age exhibited shorter long bones. Histological analysis in these mice revealed that the zone of hypertrophic chondrocytes in the growth plate was markedly expanded. In situ hybridization and quantitative real-time PCR analyses demonstrated that Matrix metalloproteinase-13 (Mmp13) and Osteopontin expression was significantly decreased, indicating impaired chondrocyte terminal differentiation. Moreover, Egr1 and Egr2, transcription factors whose expression is restricted to the last layers of hypertrophic chondrocytes in wild-type mice, were also strongly downregulated in these cKOosx mice. In transient transfection experiments in the RCS rat chondrosarcoma cell line, the expression of Egr1, Egr2, or a constitutively active mutant of MEK1 increased the activity of an Osteopontin promoter, whereas the MEK1-induced activation of the Osteopontin promoter was inhibited by the coexpression of Nab2, an Egr1 and Egr2 co-repressor. These results suggest that MEK1-ERK signaling activates the Osteopontin promoter in part through Egr1 and Egr2. Finally, our histological analysis of cKOosx mice demonstrated enchondroma-like lesions in the bone marrow that are reminiscent of human metachondromatosis, a skeletal disorder caused by mutations in PTPN11. Our observations suggest that the development of enchondromas in metachondromatosis may be caused by reduced extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK MAPK) signaling. © 2014 American Society for Bone and Mineral Research.
Regulation of Mitochondrial Morphology by Positive Feedback Interaction Between PKCδ and Drp1 in Vascular Smooth Muscle Cell
Authors: Lim Soyeon , Lee Se‐Yeon , Seo Hyang‐Hee , Ham Onju , Lee Changyeon , Park Jun‐Hee , Lee Jiyun , Seung Minji , Yun Ina , Han Sun M. , Lee Seahyoung , Choi Eunhyun , Hwang Ki‐Chul 
Published: November 2014

ABSTRACTDynamin‐related protein‐1 (Drp1) plays a critical role in mitochondrial fission which allows cell proliferation and Mdivi‐1, a specific small molecule Drp1 inhibitor, is revealed to attenuate proliferation. However, few molecular mechanisms‐related to Drp1 under stimulus for restenosis or atherosclerosis have been investigated in vascular smooth muscle cells (vSMCs). Therefore, we hypothesized that Drp1 inhibition can prevent vascular restenosis and investigated its regulatory mechanism. Angiotensin II (Ang II) or hydrogen peroxide (H2O2)‐induced proliferation and migration in SMCs were attenuated by down‐regulation of Drp1 Ser 616 phosphorylation, which was demonstrated by in vitro assays for migration and proliferation. Excessive amounts of ROS production and changes in mitochondrial membrane potential were prevented by Drp1 inhibition under Ang II and H2O2. Under the Ang II stimulation, activated Drp1 interacted with PKCδ and then activated MEK1/2‐ERK1/2 signaling cascade and MMP2, but not MMP9. Furthermore, in ex vivo aortic ring assay, inhibition of the Drp1 had significant anti‐proliferative and ‐migration effects for vSMCs. A formation of vascular neointima in response to a rat carotid artery balloon injury was prevented by Drp1 inhibition, which shows a beneficial effect of Drp1 regulation in the pathologic vascular condition. Drp1‐mediated SMC proliferation and migration can be prevented by mitochondrial division inhibitor (Mdivi‐1) in in vitro, ex vivo and in vivo, and these results suggest the possibility that Drp1 can be a new therapeutic target for restenosis or atherosclerosis. J. Cell. Biochem. 116: 648–660, 2015. © 2014 Wiley Periodicals, Inc.
Multiple goals: A review and derivation of general principles
Authors: Unsworth Kerrie , Yeo Gillian , Beck James 
Published: November 2014

SummaryA great deal of literature has examined the factors involved in single‐goal pursuit. However, there is a burgeoning realization that employees hold multiple goals at any one point in time and that findings from the single‐goal literature do not necessarily apply to multiple‐goal situations. Research is now being conducted on multiple goals, but it is being conducted across a broad range of disciplines, examining different levels of the goal hierarchy. Consequently, researchers are using the same label to refer to distinct concepts (the “jangle” fallacy) or different labels to refer to similar concepts (the “jingle” fallacy), and some aspects of the multiple‐goal space are yet to be examined. We derive seven general principles of the multiple‐goal process from a broad review of the literature. In doing so, we provide a common architecture and an overarching perspective of the theory for ongoing research as well as highlighting a number of areas for future research. Copyright © 2014 John Wiley & Sons, Ltd.
Putting your best “face” forward: The role of emotion‐based well‐being in organizational research
Authors: Wright Thomas A. 
Published: November 2014

SummaryOrganizational researchers have long been interested in the role played by employee “happiness” in workplace life. What remains less clear is what exactly constitutes happiness. The present purpose is not to invent a new definition of happiness. Rather the goal is to present the extant body of knowledge about “happiness” in a more manageable and consistent manner to assist future research endeavors. To that end, an overview of the literature is provided, which summarizes the differing approaches to the study of the “happiness” and “well‐being” constructs into a more coherent framework. More specifically, the four faces of happiness taxonomy is used to highlight the conceptual diversity of happiness. These four faces include objective indicators, eudaimonic well‐being, facets of satisfaction, and emotion‐based conceptualizations of well‐being. The present research highlights the importance of emotion‐based measures of well‐being (Category 4) in the prediction of such important organizational variables as job performance and employee retention. Several avenues for future research endeavors on workplace happiness and well‐being are suggested, including the possibility of additional faces of happiness. Copyright © 2014 John Wiley & Sons, Ltd.
Future time perspective, regulatory focus, and selection, optimization, and compensation: Testing a longitudinal model
Authors: Baltes Boris B. , Wynne Kevin , Sirabian Mgrdich , Krenn Daniel , de Lange Annet 
Published: November 2014

SummaryThis study examines the behavioral processes through which future time perspective (FTP) and regulatory focus may influence coping behaviors in older workers. A three‐wave longitudinal study was conducted to test a novel model, positing that FTP affects regulatory focus, which then influences the coping strategies of selection, optimization, and compensation. A sample of participants from the Netherlands was invited to participate and complete online questionnaires. Results demonstrated strong support that FTP was found to influence regulatory focus, which then influenced the use of selection, optimization, and compensation behaviors. Copyright © 2014 John Wiley & Sons, Ltd.
Understanding the physical environment of work and employee behavior: An affective events perspective
Authors: Ashkanasy Neal M. , Ayoko Oluremi B. , Jehn Karen A. 
Published: November 2014

SummaryDespite the fact that so many employees in the modern industrialized world work in office settings, organizational behavior researchers to date have been slow to recognize how important it is to study the effects of the physical work environment on office workers. Consequently, we have yet to form a clear understanding of the connection between the workplace physical environment and office workers' behaviors and outcomes. In this essay, therefore, we seek to unpack some of the broad issues, including the effects of major characteristics of the physical environment of work—such as personal space and spatial density, personalization and identity, territoriality, conflict, and emotions—that can help to advance our knowledge in this field. We focus on open‐plan office settings and suggest that this particular physical environment constitutes a source of “affective events” that, in turn, shape office workers' behaviors and attitudes. Copyright © 2014 John Wiley & Sons, Ltd.
Distributed MPC for resource‐constrained control systems
Authors: Scherer Helton , Camponogara Eduardo , Normey‐Rico Júlio , Álvarez José Domingo , Guzmán José Luis 
Published: November 2014

SUMMARYThis article presents a distributed model predictive control methodology to manage energy resources for a set of consumer subsystems. The objective of the controller is to optimally distribute the allowable energy to the subsystems. The proposed methodology yields a distributed solution that converges to the optimum that would be obtained by a centralized controller. This optimal performance is achieved by expressing the problem in terms of slack variables and the global coupling constraint as a set of local subsystem constraints, thereby favoring the application of distributed model predictive control. Hardware‐in‐the‐loop experiments with an air‐conditioning thermal solar plant are performed to show the good performance of the proposed distributed controller. Copyright © 2014 John Wiley & Sons, Ltd.
The Insensitive Energetic Material Trifurazano‐oxacycloheptatriene (TFO): Synthesis and Detonation Properties
Authors: Wang Xijie , Xu Kangzhen , Sun Qian , Wang Bozhou , Zhou Cheng , Zhao Fengli 
Published: November 2014

AbstractThe high‐energy insensitive compound trifurazano‐oxacycloheptatriene (TFO) was first by synthesized through special etherification. The reaction mechanism and reaction conditions were discussed. TFO has a low melting point (78.6 °C) and good compatibility. TFO is insensitive to impact and friction and has similar detonation velocity (7.7 km s−1) and detonation pressure (35.6 GPa) to RDX.
Statistical Analysis of an Inter‐Laboratory Comparison of Small‐Scale Safety and Thermal Testing of RDX
Authors: Brown Geoffrey W. , Sandstrom Mary M. , Preston Daniel N. , Pollard Colin J. , Warner Kirstin F. , Sorensen Daniel N. , Remmers Daniel L. , Phillips Jason J. , Shelley Timothy J. , Reyes Jose A. , Hsu Peter C. , Reynolds John G. 
Published: November 2014

AbstractThe Integrated Data Collection Analysis (IDCA) program has conducted a proficiency test for small‐scale safety and thermal (SSST) testing of homemade explosives (HMEs). Described here are statistical analyses of the results from this test for impact, friction, electrostatic discharge, and differential scanning calorimetry analysis of the RDX Class 5 Type II standard. The material was tested as a well‐characterized standard several times during the proficiency test to assess differences among participants and the range of results that may arise for well‐behaved explosive materials. The analyses show there are detectable differences among the results from IDCA participants. While these differences are statistically significant, most of them can be justified for comparison purposes to assess potential variability when laboratories attempt to measure identical samples using methods assumed to be nominally the same. The results presented in this report include the average sensitivity results from the IDCA participants and the ranges of values obtained. The ranges represent variation about the mean values of the tests of between 26 % and 42 %. The magnitude of this variation is attributed to differences in operator, method, and environment as well as the use of different instruments that are also of varying age. The results appear to be a good representation of results generated by the broader safety testing community based on the range of methods, instruments, and environments included in the IDCA proficiency test.
An Energetic N‐Oxide and N‐Amino Heterocycle and its Transformation to 1,2,3,4‐Tetrazine‐1‐oxide
Authors: Piercey Davin G. , Chavez David E. , Heimsch Stefanie , Kirst Christin , Klapötke Thomas M. , Stierstorfer Jörg 
Published: November 2014

AbstractThis study reports the preparation of 1‐amino‐1,2,3‐triazole‐3‐oxide (DPX2) and its transformation to 1,2,3,4‐tetrazine‐1‐oxide. DPX‐2 provides insight into a novel N‐oxide/N‐amino high‐nitrogen system, being the first energetic material in this class. The ability of this material to undergo a nitrene insertion forming 1,2,3,4‐tetrazine‐1‐oxide was also studied, and evidence for this material, the first non‐benzoannulated 1,2,3,4‐tetrazine‐1‐oxide, is presented. The existence of both of these materials opens new strategies in energetic materials design. DPX2 was characterized chemically (Infrared, Raman, NMR, X‐ray) and as a high explosive in terms of energetic performances (detonation velocity, pressure, etc.) and sensitivities (impact, friction, electrostatic). DPX‐2 was found to possess good thermal stability and moderate sensitivities, indicating the viability of N‐amino N‐oxides as a strategy for the preparation of new energetic materials.
Intrinsic flexibility of NLRP pyrin domains is a key factor in their conformational dynamics, fold stability, and dimerization
Authors: Huber Roland G. , Eibl Clarissa , Fuchs Julian E. 
Published: November 2014

AbstractNucleotide‐binding domain leucine‐rich repeat‐containing receptors (NLRs) are key proteins in the innate immune system. The 14 members of the NLRP family of NLRs contain an N‐terminal pyrin domain which is central for complex formation and signal transduction. Recently, X‐ray structures of NLRP14 revealed an unexpected rearrangement of the α5/6 stem‐helix of the pyrin domain allowing a novel symmetric dimerization mode. We characterize the conformational transitions underlying NLRP oligomerization using molecular dynamics simulations. We describe conformational stability of native NLRP14 and mutants in their monomeric and dimeric states and compare them to NLRP4, a representative of a native pyrin domain fold. Thereby, we characterize the interplay of conformational dynamics, fold stability, and dimerization in NLRP pyrin domains. We show that intrinsic flexibility of NLRP pyrin domains is a key factor influencing their behavior in physiological conditions. Additionally, we provide further evidence for the crucial importance of a charge relay system within NLRPs that critically influences their conformational ensemble in solution.