bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycogen synthase kinase 3β inhibition enhanced proliferation, migration and functional re-endothelialization of endothelial progenitor cells in hypercholesterolemia microenvironment
Authors: Cui Bin , Jin Jun , Ding Xiaohan , Deng Mengyang , Yu Shiyong , Song MingBao , Yu Yang , Zhao Xiaohui , Chen Jianfei , Huang Lan 
Published: June 2015

Hypercholesterolemia impairs the quantity and function of endothelial progenitor cell. We hypothesized that glycogen synthase kinase 3β activity is involved in regulating biological function of endothelial progenitor cells in hypercholesterolemia microenvironment. For study, endothelial progenitor cells derived from apolipoprotein E-deficient mice fed with high-fat diet were used. Glycogen synthase kinase 3β activity was interfered with glycogen synthase kinase 3β inhibitor lithium chloride or transduced with replication defective adenovirus vector expressing catalytically inactive glycogen synthase kinase 3β (GSK3β-KM). Functions of endothelial progenitor cells, proliferation, migration, secretion and network formation of endothelial progenitor cells were assessed in vitro. The expression of phospho-glycogen synthase kinase 3β, β-catenin and cyclinD1 in endothelial progenitor cells was detected by Western blot. The in vivo function re-endothelialization and vasodilation were also analyzed by artery injury model transplanted with glycogen synthase kinase 3β-inhibited endothelial progenitor cells. We demonstrated that while the proliferation, migration, network formation as well as VEGF and NO secretion were impaired in apolipoprotein E-deficient endothelial progenitor cells, glycogen synthase kinase 3β inhibition significantly improved all these functions. Apolipoprotein E-deficient endothelial progenitor cells showed decreased phospho-glycogen synthase kinase 3β, β-catenin and cyclinD1 expression, whereas these signals were enhanced by glycogen synthase kinase 3β inhibition and accompanied with β-catenin nuclear translocation. Our in vivo model showed that glycogen synthase kinase 3β inhibition remarkably increased re-endothelial and vasodilation. Taken together, our data suggest that inhibition of glycogen synthase kinase 3β is associated with endothelial progenitor cell biological functions both in vitro and in vivo. It might be an important interference target in hypercholesterolemia microenvironment.
One-year results of the ANCHOR trial of EndoAnchors for the prevention and treatment of aortic neck complications after endovascular aneurysm repair
Authors: Jordan William D , Mehta Manish , Ouriel Kenneth , Arko Frank R , Varnagy David , Joye James , Moore William M , de Vries Jean-Paul PM 
Published: June 2015

Objectives EndoAnchors have been used to address proximal aortic neck complications including type Ia endoleaks and endograft migration after endovascular aortic aneurysm repair (EVAR). Methods The study population included 100 patients with one-year follow-up in the ANCHOR study. A primary cohort ( N = 73) comprised patients who underwent EndoAnchor implantation at the time of an initial EVAR and a Revision cohort ( N = 27) included patients treated remote from EVAR. A hostile neck was defined for neck length <10 mm, neck diameter >28 mm, angulation >60°, conical configuration or significant mural thrombus or calcium. Results Baseline anatomy included neck length of 17 ± 14 mm, diameter of 27 ± 5 mm, and angulation of 35 ± 18°; 83% of patients had hostile necks. Over 18 ± 4 months of clinical follow-up, six patients (6%) underwent aneurysm-related reinterventions. There were no aneurysm ruptures. Over 13 ± 2 months of imaging follow-up, freedom from type Ia endoleak was 95% in the Primary Arm and 77% in the Revision Arm ( P = .006). Aneurysm sacs regressed > 5 mm within one year in 45% of the Primary cases and in 25% of the Revisions. Aneurysm expansion > 5 mm occurred in one revision patient. Conclusion Despite a high frequency of hostile neck anatomy, proximal neck complications were relatively infrequent after EndoAnchor use.
Monoclonal B-cell lymphocytosis and early-stage chronic lymphocytic leukemia: diagnosis, natural history, and risk stratification
Authors: Strati Paolo , Shanafelt Tait D. 
Published: June 2015

Abstract
Monoclonal B lymphocytosis (MBL) is defined as the presence of a clonal B-cell population in the peripheral blood with fewer than 5 × 109/L B-cells and no other signs of a lymphoproliferative disorder. The majority of cases of MBL have the immunophenotype of chronic lymphocytic leukemia (CLL). MBL can be categorized as either low count or high count based on whether the B-cell count is above or below 0.5 × 109/L. Low-count MBL can be detected in ∼5% of adults over the age of 40 years when assessed using standard-sensitivity flow cytometry assays. A number of biological and genetic characteristics distinguish low-count from high-count MBL. Whereas low-count MBL rarely progresses to CLL, high-count MBL progresses to CLL requiring therapy at a rate of 1% to 2% per year. High-count MBL is distinguished from Rai 0 CLL based on whether the B-cell count is above or below 5 × 109/L. Although individuals with both high-count MBL and CLL Rai stage 0 are at increased risk of infections and second cancers, the risk of progression requiring treatment and the potential to shorten life expectancy are greater for CLL. This review highlights challenging questions regarding the classification, risk stratification, management, and supportive care of patients with MBL and CLL.
Human intermittent hypoxia-induced respiratory plasticity is not caused by inflammation
Authors: Beaudin Andrew E. , Waltz Xavier , Pun Matiram , Wynne-Edwards Katherine E. , Ahmed Sofia B. , Anderson Todd J. , Hanly Patrick J. , Poulin Marc J. 
Published: June 2015

Ventilatory instability, reflected by enhanced acute hypoxic (AHVR) and hypercapnic (AHCVR) ventilatory responses is a fundamental component of obstructive sleep apnoea (OSA) pathogenesis. Intermittent hypoxia-induced inflammation is postulated to promote AHVR enhancement in OSA, although the role of inflammation in intermittent hypoxia-induced respiratory changes in humans has not been examined. Thus, this study assessed the role of inflammation in intermittent hypoxia-induced respiratory plasticity in healthy humans.In a double-blind, placebo-controlled, randomised crossover study design, 12 males were exposed to 6 h of intermittent hypoxia on three occasions. Prior to intermittent hypoxia exposures, participants ingested (for 4  days) either placebo or the nonsteroidal anti-inflammatory drugs indomethacin (nonselective cyclooxygenase (COX) inhibitor) and celecoxib (selective COX-2 inhibitor). Pre- and post-intermittent hypoxia resting ventilation, AHVR, AHCVR and serum concentration of the pro-inflammatory cytokine tumour necrosis factor (TNF)-α were assessed.Pre-intermittent hypoxia resting ventilation, AHVR, AHCVR and TNF-α concentrations were similar across all three conditions (p≥0.093). Intermittent hypoxia increased resting ventilation and the AHVR similarly across all conditions (p=0.827), while the AHCVR was increased (p=0.003) and TNF-α was decreased (p=0.006) with only selective COX-2 inhibition.These findings indicate that inflammation does not contribute to human intermittent hypoxia-induced respiratory plasticity. Moreover, selective COX-2 inhibition augmented the AHCVR following intermittent hypoxia exposure, suggesting that selective COX-2 inhibition could exacerbate OSA severity by increasing ventilatory instability.
Effects of fluid administration on renal perfusion in critically ill patients
Authors: Moussa Mouhamed Djahoum , Scolletta Sabino , Fagnoul David , Pasquier Pierre , Brasseur Alexandre , Taccone Fabio Silvio , Vincent Jean-Louis , De Backer Daniel 
Published: June 2015

Abstract
Introduction
Fluid administration is a first-line therapy for acute kidney injury associated with circulatory failure. Although aimed at increasing renal perfusion in these patients, this intervention may improve systemic hemodynamics without necessarily ameliorating intrarenal flow distribution or urine output. We used Doppler techniques to investigate the effects of fluid administration on intrarenal hemodynamics and the relationship between changes in renal hemodynamics and urine output. We hypothesized that, compared to systemic hemodynamic variables, changes in renal hemodynamics would better predict increase in urine output after fluid therapy.

Methods
We measured systemic hemodynamic variables and performed renal interlobar artery Doppler on both kidneys before and after volume expansion in 49 adult patients with acute circulatory failure. We measured systolic and diastolic velocities and computed the resistivity index (RI). We recorded urine output for 3 h before and after the fluid challenge.

Results
Fluid administration resulted in a small but consistent decrease in RI (from 0.73 ± 0.09 to 0.71 ± 0.09, p < 0.01). There was a concomitant increase in mean arterial pressure (from 75 ± 15 to 80 ± 14 mmHg, p < 0.01), pulse pressure (49 ± 19 to 55 ± 19 mmHg, p < 0.01) and urine output (55 ± 76 to 81 ± 87 ml/hour, p < 0.01). Changes in RI were negatively correlated with changes in urine output and mean arterial pressure but not in pulse pressure. The increase in urine output was predicted by changes in RI but not by changes in systemic hemodynamics.

Conclusions
Changes in renal hemodynamics during a fluid challenge can be observed by Doppler ultrasonography before urine output increases. Moreover, these changes are better predictors of an increase in urine output than are mean arterial pressure and pulse pressure.
Genetic risk variants associated with in situ breast cancer
Authors: Campa Daniele , Barrdahl Myrto , Gaudet Mia M. , Black Amanda , Chanock Stephen J. , Diver W. Ryan , Gapstur Susan M. , Haiman Christopher , Hankinson Susan , Hazra Aditi , Henderson Brian , Hoover Robert N. , Hunter David J. , Joshi Amit D. , Kraft Peter , Le Marchand Loic , Lindström Sara , Willett Walter , Travis Ruth C. , Amiano Pilar , Siddiq Afshan , Trichopoulos Dimitrios , Sund Malin , Tjønneland Anne , Weiderpass Elisabete , Peeters Petra H. , Panico Salvatore , Dossus Laure , Ziegler Regina G. , Canzian Federico , Kaaks Rudolf 
Published: June 2015

Abstract
Introduction
Breast cancer in situ (BCIS) diagnoses, a precursor lesion for invasive breast cancer, comprise about 20 % of all breast cancers (BC) in countries with screening programs. Family history of BC is considered one of the strongest risk factors for BCIS.

Methods
To evaluate the association of BC susceptibility loci with BCIS risk, we genotyped 39 single nucleotide polymorphisms (SNPs), associated with risk of invasive BC, in 1317 BCIS cases, 10,645 invasive BC cases, and 14,006 healthy controls in the National Cancer Institute’s Breast and Prostate Cancer Cohort Consortium (BPC3). Using unconditional logistic regression models adjusted for age and study, we estimated the association of SNPs with BCIS using two different comparison groups: healthy controls and invasive BC subjects to investigate whether BCIS and BC share a common genetic profile.

Results
We found that five SNPs (CDKN2BAS-rs1011970, FGFR2-rs3750817, FGFR2-rs2981582, TNRC9-rs3803662, 5p12-rs10941679) were significantly associated with BCIS risk (P value adjusted for multiple comparisons <0.0016). Comparing invasive BC and BCIS, the largest difference was for CDKN2BAS-rs1011970, which showed a positive association with BCIS (OR = 1.24, 95 % CI: 1.11–1.38, P = 1.27 x 10−4) and no association with invasive BC (OR = 1.03, 95 % CI: 0.99–1.07, P = 0.06), with a P value for case-case comparison of 0.006. Subgroup analyses investigating associations with ductal carcinoma in situ (DCIS) found similar associations, albeit less significant (OR = 1.25, 95 % CI: 1.09–1.42, P = 1.07 x 10−3). Additional risk analyses showed significant associations with invasive disease at the 0.05 level for 28 of the alleles and the OR estimates were consistent with those reported by other studies.

Conclusions
Our study adds to the knowledge that several of the known BC susceptibility loci are risk factors for both BCIS and invasive BC, with the possible exception of rs1011970, a putatively functional SNP situated in the CDKN2BAS gene that may be a specific BCIS susceptibility locus.
Cough hypersensitivity as a neuro‐immune interaction
Authors: Song Woo‐Jung , Chang Yoon‐Seok 
Published: June 2015

AbstractCough is an intrinsic protective reflex. However, chronic cough affects a considerable proportion of general population and has a major impact on quality of life. A recent paradigm shift to ‘cough hypersensitivity syndrome’ suggests that chronic cough arises from hypersensitivity of the airway sensory nerves. As cough reflex is determined by interaction of the nervous system with immune system, persistent dysregulation of one or both of these systems may lead to chronic cough hypersensitivity. Here we review the current evidence for the neuro‐immune interactions that underlie cough hypersensitivity and discuss future therapeutic strategies.
Cerebrospinal Fluid CRH Levels in Late Pregnancy Are Not Associated With New-Onset Postpartum Depressive Symptoms
Authors: Zaconeta Alberto Moreno , Amato Angélica Amorim , Barra Gustavo Barcelos , Casulari da Motta Lucília Domingues , de Souza Vinícius Carolino , Karnikowski Margô Gomes de Oliveira , Casulari Luiz Augusto 
Published: June 2015

Context:
CRH participates in the hypothalamic-pituitary-adrenal axis and in neural circuits involved in the pathophysiology of depression. During pregnancy, the placenta produces large amounts of CRH, and production ceases abruptly after delivery. The relationship between CRH in the cerebrospinal fluid (CSF) during pregnancy and peripartum mood disorders has not been investigated.

Objectives:
The objectives were to determine whether there are differences in CSF CRH concentrations of pregnant and nonpregnant women and whether CSF CRH concentrations in late pregnancy are associated with the presence of depressive symptoms during pregnancy and in the early postpartum period.

Design:
This was a prospective cohort study conducted from January to April, 2011.

Setting:
The study was conducted in one public and two private hospitals in Brasilia, Brazil.

Patients:
Patients included 107 healthy pregnant women who underwent elective cesarean delivery and 22 nonpregnant healthy women who underwent spinal anesthesia for elective surgical sterilization.

Intervention:
CRH in CSF was measured in pregnant and nonpregnant women by ELISA.

Main Outcome Measure:
The association between CSF CRH concentration at delivery and maternal depression assessed before cesarean section and postpartum (4 to 8 wk) with the Edinburgh Postnatal Depression Scale (EPDS), with a cutoff of ≥ 13.

Results:
CRH concentration in the CSF was significantly higher in pregnant (4.1 ± 0.51 log CRH) than in nonpregnant women (3.6 ± 0.26 log CRH) (P &lt; .001). Depressive symptoms starting after delivery occurred in 5.6% of women. CRH concentration in CSF was not different between women without depressive symptoms and women showing such symptoms during pregnancy or in the postpartum period.

Conclusion:
CRH concentration in the CSF was higher in pregnant women than in nonpregnant women. However, in this sample, CSF CRH in late pregnancy was not associated with new-onset depressive symptoms in the early postpartum period.
An Expedient Strategy towards an Advanced Pyrrolidine Intermediate for the Synthesis of Pyrrolizidine Alkaloids
Authors: Bera Smritilekha , Mondal Dhananjoy , Chatterjee Bhaskar 
Published: June 2015

Abstract
The asymmetric synthesis of a new pyrrolidine intermediate and a preceding amino triol derivative was accomplished using Crimmins’ modified Evans aldol reaction as the key step. The synthetically useful pyrrolidine intermediate is expected to serve as the immediate precursor of biologically important pyrrolizidine alkaloids (−)-isoretronecanol and (−)-cremastrine. In addition, the amino triol subunit acts as a useful intermediate for the synthesis of oxocane heterocycles.
Hydrothermal Synthesis of Fluorescent Carbon Dots towards Ion Response and Silk Screen Patterns
Authors: Huang Likai , Yang Shengyang , Chen Li , Chen Su 
Published: June 2015

Abstract
In this work, we report a one-step hydrothermal reaction to fabricate fluorescent carbon dots (CDs) using polyimide (PI) as precursor. The as-prepared CDs exhibit bright blue-green fluorescence and a favorable quantum yield of ca. 20%. Also, the fluorescent CDs show both pH dependence and an ion response towards metal ions, as well as a fluorescence shift after being passivated by various organic amines. In addition, the CDs can be used as fluorescent inks to achieve fluorescent patterns for promising applications in anticounterfeit and optoelectronic devices.
Designed Synthesis of Porphyrin-based Two-dimensional Covalent Organic Frameworks with Highly Ordered Structures
Authors: Chen Xiong , Gao Jia , Jiang Donglin 
Published: June 2015

Abstract
Porphyrins are representative functional π-systems; synthesizing their highly ordered structures is an established goal in chemistry. Here, we report the designed synthesis of a series of porphyrin-based covalent organic frameworks with highly ordered structures through polycondensation. The porphyrin-based frameworks exhibited high crystallinity, high porosity, and extended π-conjugation over the polyporphyrin sheets.
Bis(terpyridine)iron(II) Complex Wires with a Bithiophene Linker for Superior Long-range Electron Transport
Authors: Bajpayee Aayushi , Maeda Hiroaki , Katagiri Shunsuke , Sakamoto Ryota , Nishihara Hiroshi 
Published: June 2015

Abstract
Fabrication of bis(terpyridine)iron(II) complex wires by introduction of a bithiophene-linked bridging ligand (LBT) on the Au(111) surface is discussed. A comparative study revealed a very small energy gap between the HOMOs of Fe(tpy)2 and LBT indicating relatively strong electronic coupling and better electron-transport ability. By cyclic voltammetry and potential step chronoamperometric measurements, the small attenuation factor (βd = 0.010 ± 0.004 Å−1) was witnessed.
A GIS Based Integration Method for Geothermal Resources Exploration and Site Selection
Authors: Noorollahi Younes , Ghasempour Roghayeh , Jalilinasrabady Saeid 
Published: June 2015

A toolbox using a Geographic Information System (GIS) was developed and introduced as a decision-making tool to locate potential geothermal areas. The study aims to introduce step-by-step guidelines and develop a user friendly computer program that can determine promising geothermal areas. Six data layers characterizing the geothermal area are employed in the site selection process. ArcMap was used as a base program to develop the GIS Model for Geothermal Resource Exploration (GM-GRE), which consists of geoprocessing tools and a model builder. Criteria for defining promising areas based on each layer are defined using spatial relationship analysis of currently producing geothermal wells in Akita and Iwate prefectures in northern Japan as proven geothermal resources and evidence layers. Areas with geothermal potential were defined and prioritized using input data layers.
Interactive Interoperability Between Firefighters and Fire Protection Equipment
Authors: Horová Kamila , Entler Slavomír , Wald František 
Published: June 2015

In 2005 coal handling bridges in Opatovice power station, Czech Republic, were damaged by fire. Effects of the fire limited coal supply for 3 months. Following that fire the new fire protection equipment have been gradually installed in other Czech power plants.
In 2009 the complex automatic fire protection equipment of coal handling route was installed into operation in Tušimice power plant. However, after starting the operation it showed that activation of the extinguishing system on the inclined conveyor bridge threatened the health and life of fire-fighters conducting an intervention.
In this paper an interactive algorithm that ensures a flexible cooperation intervening fire-fighters and automatic extinguishing system without a risk of fire-fighters life is investigated. To confirm the applicability of the interoperability algorithm possible fire scenario is analysed in NIST code FDS. Development of gas temperatures in strong chimney flow gives also a view into part of mechanical response of structure.
Prevention of dehydration in hospital inpatients
Authors: Johnstone Philippa , Alexander Ratan , Hickey Nick 
Published: June 2015

Background: The adverse effects of dehydration in hospital inpatients can be severe, resulting in morbidity and even mortality. This study examined the ability of surgical patients in a district general hospital to undertake the seemingly minor but critically important task of taking on oral fluids using the conventional, hospital-supplied, water jug and glass. Methods: Surgical patients receiving oral fluids were interviewed on two occasions. A questionnaire assessed the patient's capacity to use the water jug and recorded his or her experience. The patient's ability to use jugs with varying volumes of water was then observed. Results: Some patients simply could not physically lift the conventional jug of water and often went thirsty; 42% of patients could not reach the jug of water at the time of interview; 31% of patients found the simple task of pouring a glass of water difficult, very difficult or impossible. Conclusion: Patients need their individual requirements to be assessed and their preferences addressed on admission to hospital, by means of a fluid-assessment chart. Alternative methods of hydration should also be considered. Education and training on the importance of hydration remain key. These small changes could improve patient hydration and independence, thus preventing avoidable harm.
Non-invasive fetal ABO genotyping in maternal plasma using real-time PCR
Authors: Song Wenqian , Zhou Shihang , Shao Linnan , Wang Ni , Pan Lingzi , Yu Weijian 
Published: June 2015

AbstractFetal-maternal ABO incompatibility is a frequent cause of hemolytic disease of the fetus and newborn (HDFN). The routine serological testing of maternal IgG antibody level to predict HDFN shows low reliability. Non-invasive fetal ABO genotyping could provide a new avenue for predicting ABO-HDFN in early pregnancy. The aim of our study is to investigate the feasibility of fetal ABO genotyping in maternal plasma with real-time PCR.Plasma samples were collected from a total of 73 blood group O pregnant women between 12 and 25 weeks of gestation, and then DNA was extracted from the maternal plasma containing cell-free fetal DNA (cffDNA). TaqMan-based real-time PCR was performed after methylation-sensitive restriction enzyme to detect hypermethylatedThe fetalWe have developed a rapid and reliable protocol for fetal ABO genotyping in maternal plasma using real-time PCR. This protocol is suitable for routine prenatal diagnose of HDFN and forensic analysis.