bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Role of the Environment in Human Life in the Novels Tambora 1815 and Medulla Sinculasis by Paox Iben Mudhaffar
Authors: Hendri  , Wahidatul Murtafi’ah  
Published: August 2024

The purpose of this research is to describe (1) the form of environmental role in human life, (2) the form of human attitude to the environment, (3) the form of local wisdom in its relation with environment in novel Medulla Sinculasis and Tambora 1815 by Paox Iben Mudhaffar. This research was qualitative descriptive. The source of data was the novels Medulla Sinculasis and Tambora 1915 by Paox Iben Mudhaffar. The collection of the data used document content analysis (read-note-analysis). Analysis of the data in this study is a qualitative descriptive analysis technique is done by categorizing, describing, and conclusion. In this research, the researchers chose the novels Medulla Sinculasis and Tambora 1815 as data sources considering that these novels were the most appropriate to study among Paox Iben's other works. The Tambora 1815 novel used is the first printed novel, March 2015 published by Bukudaku Addressra Publishing, Mataram with a book thickness of 301 pages, ISBN: 978-979-18863-1-4 and the Medulla Sinculasis novel used is the first printed novel, January 2011 published by IruS (Rumah Arus Institute) Mataram, Lombok with a book thickness of 324 pages, ISBN: 979-1698-045-94-7. The results of this research are as follows. First, the role of environment represented in the novels Medulla Sinculasis and Tambora 1815 consists of : (a) the environment as the source of food, (b) the environment as the source of medicines, (c) the environment as the source of building material, (d) the environment as living place, (e) the environment as the provider of payment tool (barter), (f) the environment as a means of livelihood, (g) the environment as the source of water, (h) the environment as the provider of things for means of transportation, and (i) the environment as tourist object.
Perancangan dan Implementasi Sistem Informasi Launchpad Cryptocurrency pada PT. Pintar Media Teknologi
Authors: Suwarno Suwarno , Valentino Elvin 
Published: August 2024

Teknologi blockchain telah menjadi fokus utama dalam pengembangan aplikasi web terdesentralisasi, membuka era baru dalam evolusi internet yang dikenal sebagai Web3. Di tengah perkembangan ini, perusahaan startup PT. Pintar Media Teknologi memperkenalkan aplikasi launchpad cryptocurrency yang mengandalkan teknologi blockchain untuk memberikan solusi terdesentralisasi dalam perdagangan aset crypto. Penulis mendapatkan kesempatan untuk melakukan penelitian pada PT. Pintar Media Teknologi untuk membangun aplikasi launchpad cryptocurrency. Metodologi pengembangan aplikasi blockchain yang diterapkan adalah metodologi Agile Scrum. Melalui analisis fitur-fitur yang dikembangkan, seperti perancangan Entity Relationship Diagram, desain basis data, hingga implementasi fitur-fitur utama seperti autentikasi menggunakan Web3, manajemen token, dan manajemen launchpad. Hasil penelitian ini memberikan wawasan tentang praktik pengembangan aplikasi blockchain dan kontribusi penulis pada penelitian ini adalah merancang dan mengimplementasi sistem informasi launchpad cryptocurrency sesuai dengan kebutuhan para stakeholders sehingga dapat mempercepat proses pengembangan produk.
Fungi-sensitized individuals have unique profiles where Alt a 1 dominates promoting response to grass, ragweed and cat allergens
Authors: Kalyniuk Viktoriia , Rodinkova Victoria , Yuriev Serhii , Mokin Vitalii , Losenko Arsen , Kryvopustova Mariia , Zabolotna Diana , Gogunska Inna 
Published: August 2024

IntroductionThe aim of our work was to determine comprehensively the sensitization profile of patients hypersensitive to fungal allergenic components in the Ukrainian population, identifying features of their co-sensitization to allergens of other groups and establishing potential relationships between causative allergens and their ability to provoke this hypersensitivity.MethodsA set of programs was developed using Python and R programming languages, implementing the K-means++ clustering method. Bayesian networks were constructed based on the created clusters, allowing for the assessment of the probabilistic interplay of allergen molecules in the sensitization process of patients.Results and discussionIt was found that patients sensitive to fungi are polysensitized, with 84.77% of them having unique allergological profiles, comprising from 2 to several dozen allergens from different groups. The immune response to Alt a 1 may act as the primary trigger for sensitization to other allergens and may contribute to a high probability of developing sensitivity to grasses (primarily to Phl p 2), ragweed extract, and the Amb a 1 pectate lyase, as well as to pectate lyase Cry j 1 and cat allergen Fel d 1. Individuals polysensitized to molecular components of fungi were often sensitive to such cross-reactive molecules as lipocalins Fel d 4 and Can f 6, as well. Sensitivity to Ambrosia extract which dominated in the development of sensitization to ragweed pollen indicating the importance of different allergenic components of this plant's pollen. This hypothesis, along with the assumption that Phl p 2 may be the main trigger for sensitivity to grasses in patients with Alternaria allergy, requires further clinical investigation.
Water filter: a rapid water environmental DNA collector in the field
Authors: Wu Ping , Feng Jie , Ju Mingxia , Wu Shenhao , Han Weichun , Wang Miao , Liao Junquan , Zhao Lifeng , Gao Yifan , Zheng Jiao , Luo Mingjie , Gong Huixian , Zeng Lidong , Lai Juan , Li Mingze , Yan Qin , Sun Lei , Liu Yongfeng 
Published: August 2024

Biological monitoring using environmental DNA (eDNA) technology has expanded from micro- to macro-organisms. In aquatic eDNA studies, large volumes of water need to be filtered rapidly in the field, which requires development of effective eDNA collection devices. In this study, we introduce a novel portable eDNA collection system containing a GM dual-channel water filter and a DNA extraction kit adapted to large filter membranes (ø 100 mm). The water filter is powered by a high-capacity lithium battery (9,000 mA), which operates two peristaltic pumps and maintains a continuous filtration rate of up to 1 L/min for 5 h in outdoor settings. For sample collection, the optimum conditions are still water and turbidity below 8 nephelometric turbidity units. This allows for the filtration of 10 L of water within 10 min by use of a 0.22-μm filter. Metagenomic and 12S metabarcoding sequencing showed that the DNA extraction quality and species annotation accuracy of our custom DNA extraction kit, which was tailored for this system, rivaled the performance of established kits. The GM water filter’s enrichment mode gave consistent results with vacuum filtration, which greatly reduced the filtration time for large water samples, while accurately reproducing species annotations. This innovation streamlines the eDNA collection and annotation process and offers substantial benefits for biodiversity monitoring and conservation efforts.
HMGB2 drives tumor progression and shapes the immunosuppressive microenvironment in hepatocellular carcinoma: insights from multi-omics analysis
Authors: Chen Yan-zhu , Meng Zhi-shang , Xiang Zuo-lin 
Published: August 2024

BackgroundHepatocellular carcinoma (HCC) poses a significant health burden globally, with high mortality rates despite various treatment options. Immunotherapy, particularly immune-checkpoint inhibitors (ICIs), has shown promise, but resistance and metastasis remain major challenges. Understanding the intricacies of the tumor microenvironment (TME) is imperative for optimizing HCC management strategies and enhancing patient prognosis.MethodsThis study employed a comprehensive approach integrating multi-omics approaches, including single-cell RNA sequencing (scRNA-seq), bulk RNA sequencing (Bulk RNA-seq), and validation in clinical samples using spatial transcriptomics (ST) and multiplex immunohistochemistry (mIHC). The analysis aimed to identify key factors influencing the immunosuppressive microenvironment associated with HCC metastasis and immunotherapy resistance.ResultsHMGB2 is significantly upregulated in HCCTrans, a transitional subgroup associated with aggressive metastasis. Furthermore, HMGB2 expression positively correlates with an immunosuppressive microenvironment, particularly evident in exhausted T cells. Notably, HMGB2 expression correlated positively with immunosuppressive markers and poor prognosis in HCC patients across multiple cohorts. ST combined with mIHC validated the spatial expression patterns of HMGB2 within the TME, providing additional evidence of its role in HCC progression and immune evasion.ConclusionHMGB2 emerges as a critical player of HCC progression, metastasis, and immunosuppression. Its elevated expression correlates with aggressive tumor behavior and poor patient outcomes, suggesting its potential as both a therapeutic target and a prognostic indicator in HCC management.
Protocol for a systematic review and individual participant data meta-analysis of optimizing oxygen therapy in critically ill patients
Authors: Yang Xiaobo , Ouyang Yaqi , Xu Jiqian , Shang You 
Published: August 2024

BackgroundOxygen therapy is a cornerstone treatment of critically ill patients in the intensive care unit (ICU). Whether lower oxygenation therapy brings superior survival outcomes to higher oxygenation therapy is unknown.MethodsWe will search electronic databases: PubMed, Embase, Web of Science, the Cochrane Central Register of Controlled Trials (CENTRAL), International Clinical Trials Registry Platform (ICTRP), and ClinicalTrials.gov from inception to 1 January 2024. Two authors will independently screen for all eligible clinical studies. Emails will be sent for individual participant data. The statistical analyses will be conducted using STATA 15.0 software.ResultsWe will evaluate the efficacy of lower oxygenation therapy compared with higher oxygenation therapy based on individual participant data.ConclusionThis study will offer clinical evidence for oxygen therapy in ICU patients.
Effect of green banana and pineapple fibre powder consumption on host gut microbiome
Authors: Chong Chun Wie , Liew Mei Shan , Ooi Weitze , Jamil Hassan , Lim Angie , Hooi Suet Li , Tay Clarisse S. C. , Tan Gwendoline 
Published: August 2024

PurposeTo determine whether green banana powder (GBP) and pineapple fibre powder (PFP) promote beneficial bacterial species, directly improve human gut health and modulate the gut microbiome and understand their utility as functional foods and dietary supplements.MethodsOver 14 days, 60 adults followed protocol requirements, completed food diaries and study questionnaires, avoided consuming supplements with prebiotics, probiotics or postbiotics, and ingested food containing 5 g of total daily fibre [placebo (10.75 g), GBP (10.75 g) or PFP (7.41 g)]. Participants’ medical and baseline wellness histories, as well as stool samples, were collected at baseline, day 7 and 14. Stool DNA was processed for sequencing.ResultsDietary fibre and resistant starches (RS) in GBP and PFP promoted temporal increases in beneficial bacteria. GBP significantly elevated 7 species (F. prausnitzii, B. longum, B. bifidum, B. adolescentis, B. pseudocatenulatum, B. obeum, and R. inulinivorans), while PFP enriched 6 species (B. ovatus, B. cellulosilyticus, B. bifidum, B. intestinalis, R. inulinivorans, and E. siraeum). These bacteria, found to be deficient in younger adults, were promoted by both powders. PFP benefitted both genders aged 16–23, while GBP benefitted overweight/obese individuals, including females. GBP and PFP fiber and RS improved bowel regularity and health as well as metabolism by promoting histidine, branched-chain amino acids, short-chain fatty acids, and biotin production. The additional fiber caused “low” bloatedness and reduced “fairly bad” sleep disruptions, without affecting sleep durations.ConclusionGBP and PFP supplementation increased beneficial bacteria and metabolites, improved host gut health, and present a valuable nutritional strategy for enhancing human health.Clinical trial registrationAMILI Institutional Review Board, Identifier 2023/0301.
Acetylcytidine modification of DDX41 and ZNF746 by N-acetyltransferase 10 contributes to chemoresistance of melanoma
Authors: Wang Li , Zeng Yuefen , Zhang Ying , Zhu Yun , Xu Shuangyan , Liang Zuohui 
Published: August 2024

BackgroundRapidly developed chemoresistance to dacarbazine (DTIC) is a major obstacle in the clinical management of melanoma; however, the roles and mechanisms of epi-transcriptomic RNA modification in this process have not been investigated.MethodDTIC-resistant (DR) melanoma cells were established for bulk RNA sequencing. The expressions of mRNAs were detected using qRT-PCR, and protein levels were determined using Western blotting and immunohistochemistry. Acetylated RNAs were detected by dot blotting and immunoprecipitation sequencing (acRIP-seq). A lung metastasis mouse model of melanoma was established to evaluate the anti-melanoma effects in vivo.ResultsWe identified that the expression of N-acetyltransferase 10 (NAT10), a catalytic enzyme for the N4-acetylcytidine (ac4C) modification of RNA, was significantly upregulated in the DR cells. Clinically, NAT10 expression was elevated in disease progression samples and predicted a poor outcome. Using ac4C RNA immunoprecipitation (ac4C-RIP), we found that the mRNAs of two C2H2 zinc finger transcriptional factors, DDX41 and ZNF746, were targets of NAT10-mediated ac4C modification. Gain- and loss-of-function experiments in NAT10, or in DDX41 and ZNF746, altered the chemosensitivity of melanoma accordingly, and the two target genes also negatively correlated with clinical outcomes. Finally, pharmacological inhibition of NAT10 with Remodelin sensitized melanoma cells to DTIC treatment in vitro and in a mouse xenograft model.ConclusionOur study elucidates the previously unrecognized role of NAT10-mediated ac4C modification in the chemoresistance of melanoma and provides a rationale for developing new strategies to overcome chemoresistance in melanoma patients.
Transcriptome analysis to explore the mechanism of downregulated TNIK influencing the effect of risperidone
Authors: Yuan Ruixue , Li Yaojing , Li Xiangyi , Fu Yingmei , Ning Ailing , Wang Dongxiang , Zhang Ran , Yu Shunying , Xu Qingqing 
Published: August 2024

BackgroundRisperidone is one of the most reliable and effective antipsychotics for schizophrenia treatment. However, the mechanism of action of risperidone is not yet fully understood. Traf2 and Nck-interacting protein kinase (TNIK), a schizophrenia susceptibility gene, is associated with risperidone treatment response. Our previous in vitro experiments confirmed that downregulated TNIK affected the effect of risperidone on downstream targets. However, the effect of downregulated TNIK on risperidone-induced molecular expression remains to be further explored.MethodsTranscriptome analysis was performed on U251 cells subjected to risperidone, TNIK siRNA, and no treatment, respectively. Compared to the no-treatment group, two groups of DEGs were screened out and then intersected with the schizophrenia-related genes to screen the cross-talk genes. Those DEGs were analyzed using GO and KEGG. STRING and Cytoscape were used to construct a protein-protein interaction (PPI) network for the cross-talk gene.ResultsThe results showed that the parathyroid hormone synthesis, secretion, and action were significantly enriched after risperidone treatment. Downregulated TNIK could have an impact on the collagen-containing extracellular matrix, signaling receptor activator activity, and PI3K-Akt signaling pathway. Interestingly, bone mineralization function and calcium signaling pathway were enriched in the cross-talk genes. Additionally, FGFR2, FGF1, and FGFR might be the potential targets for TNIK affecting the effects of risperidone.ConclusionThe study indicated that risperidone primarily influences functions and/or pathways associated with bone metabolism, potentially contributing to the adverse effect of osteoporosis. Our study may offer a novel perspective on investigating the mechanisms underlying the adverse effects of risperidone.
Comprehensive understanding of the adverse effects associated with temozolomide: a disproportionate analysis based on the FAERS database
Authors: Zhou Yusen , Jia Peng , Fang Yuting , Zhu Wei , Gong Yong , Fan Tianyu , Yin Jiangliu 
Published: August 2024

BackgroundTemozolomide, which is the standard drug for glioma treatment, has several Adverse events (AEs) in the treatment of gliomas and other tumors that are not yet fully understood. This is due to the pharmacological nature of the alkylating agent. A significant proportion of these effects have not been systematically documented or reported.MethodsWe selected data from the United States FDA Adverse Event Reporting System (FAERS) database from the first quarter of 2004 to the fourth quarter of 2023. Four algorithms were used for disproportionate analysis, with the objective of assessing the association between temozolomide and related adverse events.ResultsIn this study, 20,079,906 case reports were collected from the FAERS database, of which 15,152 adverse events related to temozolomide were reported. A total of 352 preferred terms (PTs) and 24 system organ classes (SOCs) that were significantly disproportionally related to the four algorithms were included. The SOCs included blood and lymphatic system disorders (χ2 = 18,220.09, n = 4,325); skin and subcutaneous tissue disorders (χ2 = 408.06, n = 1,347); investigations (χ2 = 639.44, n = 3,925); musculoskeletal and connective tissue disorders (χ2 = 1,317.29, n = 588); and psychiatric disorders (χ2 = 1,098.47, n = 877). PT levels were screened for adverse drug reaction signals consistent with drug inserts, such as anemia, thrombocytopenia, liver function abnormalities, nausea and vomiting, as well as rarely reported adverse drug reactions, such as aplastic anemia, myelodysplastic syndromes, electrolyte disorders, cerebral edema, and high-frequency mutations.ConclusionThe results of our investigation demonstrated both adverse effects that had been reported and a multitude of unreported adverse effects that were serious in nature and lacked a clear cause. These novel findings suggest that more attention should be given to the clinical conditions of patients after treatment to provide a more comprehensive perspective and understanding for further clarifying the safety of temozolomide.
Prevalence and risk factors for anxiety in patients with early- and middle-stage lung cancer: a cross-sectional study
Authors: Zhang Ruoqi , Zhang Peitong , Lin Yuejie , Guo Xiuwei , Wang Jing 
Published: August 2024

ObjectiveLung cancer is a leading cause of cancer-related morbidity and mortality worldwide, with patients frequently experiencing significant psychological distress, particularly anxiety. Despite the high prevalence of anxiety in patients with cancer, there is limited comprehensive research focusing on the specific factors influencing anxiety in patients with early- and middle-stage lung cancer within the context of Chinese medicine hospitals. Therefore, we aimed to investigate the epidemiology and factors influencing anxiety disorders in patients with early- and middle-stage primary bronchial lung cancer through a cross-sectional study.MethodsA total of 340 patients with early and middle-stage lung cancer admitted to the outpatient ward of the oncology department at Guang’anmen Hospital from June 2023 to December 2023 were included in this study. Survey data, including the patients’ general condition questionnaire, Generalized Anxiety Scale (GAD-7), Hospital Anxiety and Depression Scale (HADS), and Mental Toughness Scale (CD-RISC-10), were collected and recorded in a database using a two-person input format. Data analysis was performed using SPSS 27.0 software.ResultsOut of the 340 patients with early- and middle-stage lung cancer included in this study, 133 had anxiety, resulting in an overall anxiety detection rate of 39.12%. The chi-square test showed that statistically significant differences in religion, marital status, surgical treatment, tobacco use, and alcohol history between the anxious and non-anxious groups (p &lt; 0.05). Moreover, statistically significant differences were observed in per capita annual family income, pathological type, VAS score, targeted therapy, treatment stage, and mental toughness level (p &lt; 0.001). Other factors were not significantly correlated with anxiety onset. Multivariate logistic regression analysis showed that higher per capita family income and completed treatment independently acted as protective factors against anxiety onset in patients with early- and middle-stage lung cancer. Conversely, rare pathological types, increased pain severity, and lower levels of mental toughness were identified as independent risk factors for anxiety onset in these patients.ConclusionAnxiety was prevalent in patients with early- and middle-stage lung cancers. Rare pathological types, increased pain severity, and lower levels of mental toughness were independent risk factors for anxiety. Therefore, clinicians and psychologists should pay more attention to patients with rare types of tumors, actively manage their pain symptoms, and consider implementing mental resilience training to improve patients’ mental toughness.
Research on China’s adolescent mental health policies — analysis based on PMC index model
Authors: Yang Chengning 
Published: August 2024

IntroductionIn recent years, the suicide rate among adolescents in China has shown a continuous upward trend, and mental health issues such as depression and excessive anxiety have become increasingly prominent. Awareness and concerns around poor mental health in adolescents is rising among the general public and in academic circles, but there is little research on adolescent mental health policies in China.MethodsThis article reviews the national policies on adolescent mental health from 2000 to 2023, and analyzes and evaluates the selected policy texts through the PMC index model.ResultsThe study indicates growing national attention towards adolescent mental health policies in terms of both quantity and quality, with improved policy feasibility and synergy. However, shortcomings exist in the policy formulation process, including a lack of advocacy and supervision-oriented policies, a focus on short to medium-term effects, and inadequate comprehensive planning, hindering their swift implementation.DiscussionIn conclusion, facing the escalating crisis of adolescent mental health, the previous requirements of the education system are no longer sufficient. The government needs to further improve the top-down policy system, weave a safety net for mental health education and preventive intervention, and effectively promote the development of adolescent mental health.