bluuebunny/crossref_metadata_2025_split huggingface dataset:
Type of Homogenization and Fat Loss during Continuous Infusion of Human Milk
Authors: García-Lara Nadia Raquel , Escuder-Vieco Diana , Alonso Díaz Clara , Vázquez Román Sara , De la Cruz-Bértolo Javier , Pallás-Alonso Carmen Rosa 
Published: August 2014

Background: Substantial fat loss may occur during continuous feeding of human milk (HM). A decrease of fat loss has been described following homogenization. Well-established methods of homogenization of HM for routine use in the neonatal intensive care unit (NICU) would be desirable. Objective: We compared the loss of fat based on the use of 3 different methods for homogenizing thawed HM during continuous feeding. Methods: Sixteen frozen donor HM samples were thawed, homogenized with ultrasound and separated into 3 aliquots (“baseline agitation,” “hourly agitation,” and “ultrasound”), and then frozen for 48 hours. Aliquots were thawed again and a baseline agitation was applied. Subsequently, aliquots baseline agitation and hourly agitation were drawn into a syringe, while ultrasound was applied to aliquot ultrasound before it was drawn into a syringe. The syringes were loaded into a pump (2 mL/h; 4 hours). At hourly intervals the hourly agitation infusion was stopped, the syringe was disconnected and gently shaken. During infusion, samples from the 3 groups were collected hourly for analysis of fat and caloric content. Results: The 3 groups of homogenization showed similar fat content at the beginning of the infusion. For fat, mean (SD) hourly changes of −0.03 (0.01), −0.09 (0.01), and −0.09 (0.01) g/dL were observed for the hourly agitation, baseline agitation, and ultrasound groups, respectively. The decrease was smaller for the hourly agitation group ( P < .001). Conclusion: When thawed HM is continuously infused, a smaller fat loss is observed when syringes are agitated hourly versus when ultrasound or a baseline homogenization is used.
A systematic review of causes of sudden and severe headache (Thunderclap Headache): should lists be evidence based?
Authors: Devenney Emma , Neale Hazel , Forbes Raeburn B 
Published: August 2014

AbstractBackgroundThere are many potential causes of sudden and severe headache (thunderclap headache), the most important of which is aneurysmal subarachnoid haemorrhage. Published academic reviews report a wide range of causes. We sought to create a definitive list of causes, other than aneurysmal subarachnoid haemorrhage, using a systematic review.MethodsSystematic Review of EMBASE and MEDLINE databases using pre-defined search criteria up to September 2009. We extracted data from any original research paper or case report describing a case of someone presenting with a sudden and severe headache, and summarized the published causes.ResultsOur search identified over 21,000 titles, of which 1224 articles were scrutinized in full. 213 articles described 2345 people with sudden and severe headache, and we identified 6 English language academic review articles. A total of 119 causes were identified, of which 46 (38%) were not mentioned in published academic review articles. Using capture-recapture analysis, we estimate that our search was 98% complete. There is only one population-based estimate of the incidence of sudden and severe headache at 43 cases per 100,000. In cohort studies, the most common causes identified were primary headaches or headaches of uncertain cause. Vasoconstriction syndromes are commonly mentioned in case reports or case series. The most common cause not mentioned in academic reviews was pneumocephalus. 70 non-English language articles were identified but these did not contain additional causes.ConclusionsThere are over 100 different published causes of sudden and severe headache, other than aneurysmal subarachnoid haemorrhage. We have now made a definitive list of causes for future reference which we intend to maintain. There is a need for an up to date population based description of cause of sudden and severe headache as the modern epidemiology of thunderclap headache may require updating in the light of research on cerebral vasoconstriction syndromes.
Lipoic acid plays a role in scleroderma: insights obtained from scleroderma dermal fibroblasts
Authors: Tsou Pei-Suen , Balogh Beatrix , Pinney Adam J , Zakhem George , Lozier Ann , Amin M Asif , Stinson William A , Schiopu Elena , Khanna Dinesh , Fox David A , Koch Alisa E 
Published: August 2014

Abstract
Introduction
Systemic sclerosis (SSc) is a connective tissue disease characterized by fibrosis of the skin and organs. Increase in oxidative stress and platelet-derived growth factor receptor (PDGFR) activation promote collagen I (Col I) production, leading to fibrosis in SSc. Lipoic acid (LA) and its active metabolite dihydrolipoic acid (DHLA) are naturally occurring thiols that act as cofactors and antioxidants, and are produced by lipoic acid synthetase (LIAS). The goal of this study was to examine whether LA and LIAS was deficient in SSc patients and determine the effect of DHLA on the phenotype of SSc dermal fibroblasts. N-acetylcysteine (NAC), a commonly used thiol antioxidant, was included as a comparison.

Methods
Dermal fibroblasts were isolated from healthy subjects and patients with diffuse cutaneous SSc. Matrix metalloproteinase (MMPs), tissue inhibitors of MMPs (TIMP), plasminogen activator inhibitor-1 (PAI-1) and LIAS were measured by ELISA. The expression of Col I was measured by immunofluorescence, hydroxyproline assay, and quantitative PCR. PDGFR phosphorylation and α-smooth muscle actin (α-SMA) was measured by Western blotting. Student’s t-tests were performed for statistical analysis and p-values of less than 0.05 with two-tailed analysis were considered statistically significant.

Results
The expression of LA and LIAS in SSc dermal fibroblasts was lower than normal fibroblasts, however LIAS was significantly higher in SSc plasma and appeared to be released from monocytes. DHLA lowered cellular oxidative stress, and decreased PDGFR phosphorylation, Col I, PAI-1, and α-SMA expression in SSc dermal fibroblasts. It also restored the activities of phosphatases that inactivated the PDGFR. SSc fibroblasts produced lower levels of MMP-1 and 3, and DHLA increased them. In contrast, TIMP-1 levels were higher in SSc but DHLA had minimal effect. Both DHLA and NAC increased MMP-1 activity when SSc cells were stimulated with PDGF. In general, DHLA showed better efficacy than NAC in most cases.

Conclusions
DHLA not only acts as an antioxidant but also an antifibrotic since it has the ability to reverse the profibrotic phenotype of SSc dermal fibroblasts. Our study suggests that thiol antioxidants, including NAC and LA/DHLA, could be beneficial for patients with SSc.
Deletion of glucose oxidase changes the pattern of organic acid production in Aspergillus carbonarius
Authors: Yang Lei , Lübeck Mette , Lübeck Peter S 
Published: August 2014

Abstract

Aspergillus carbonarius has potential as a cell factory for the production of different organic acids. At pH 5.5, A.carbonarius accumulates high amounts of gluconic acid when it grows on glucose based medium whereas at low pH, it produces citric acid. The conversion of glucose to gluconic acid is carried out by secretion of the enzyme, glucose oxidase. In this work, the gene encoding glucose oxidase was identified and deleted from A. carbonarius with the aim of changing the carbon flux towards other organic acids. The effect of genetic engineering was examined by testing glucose oxidase deficient (Δgox) mutants for the production of different organic acids in a defined production medium. The results obtained showed that the gluconic acid accumulation was completely inhibited and increased amounts of citric acid, oxalic acid and malic acid were observed in the Δgox mutants.
Toxicity of nanomaterials; an undermined issue
Authors: Mogharabi Mehdi , Abdollahi Mohammad , Faramarzi Mohammad Ali 
Published: August 2014

Abstract
Nanomaterials are employed in extensive variety of commercial products such as electronic components, cosmetics, food, sports equipment, biomedical applications, and medicine. With the increasing utilization of engineered nanomaterials, the potential exposure of human to nanoparticles is rapidly increasing. Nowadays when new nanomaterials with new applications are introduced, mostly good and positive effects are mentioned whereas possible hazards arising from nanosize of the compounds are undermined. Toxicology studies of nanomaterials demonstrate some adverse effects in some human organs such as central nerve system, immune system, and lung. There is lack of complete information about human toxicity and environmental waste of nanomaterials. We aimed to highlight current toxicological concerns of potentially useful nanomaterials which are now used in pharmaceutical and biomedical sciences.
Negative ions detection in air using nano field-effect-transistor (nanoFET)
Authors: Seo Yeong-Tai , Lee Kook-Nyung , Jang Kuk Jin , Lee Min-Ho , Lee HyungSu , Seong WooKyeong , Kim Yong-Kweon 
Published: August 2014

Abstract
We firstly demonstrated the detection of anions in air using a nano field-effect transistor (nanoFET) device. Negative ions in air charged the top surface of the silicon nanoFET channel affecting the fieldeffect and making a conductance change of the channel proportional to anion concentration around the nano channel sensing surface. The real-time detection of anions in air with the nanoFET was performed for various anion concentrations which were differentiated by the distance of the anion generator to the nanoFET sensor. The air anions detection characteristics of the nanoFET device were evaluated with sensitivity and conductance change rates analysis.
Repair-Related Activation of Hedgehog Signaling in Stromal Cells Promotes Intrahepatic Hypothyroidism
Authors: Bohinc Brittany N. , Michelotti Gregory , Xie Guanhua , Pang Herbert , Suzuki Ayako , Guy Cynthia D. , Piercy Dawn , Kruger Leandi , Swiderska-Syn Marzena , Machado Mariana , Pereira Thiago , Zavacki Ann Marie , Abdelmalek Manal , Diehl Anna Mae 
Published: August 2014

Abstract
Thyroid hormone (TH) is important for tissue repair because it regulates cellular differentiation. Intrahepatic TH activity is controlled by both serum TH levels and hepatic deiodinases. TH substrate (T4) is converted into active hormone (T3) by deiodinase 1 (D1) but into inactive hormone (rT3) by deiodinase 3 (D3). Although the relative expressions of D1 and D3 are known to change during liver injury, the cell types and signaling mechanisms involved are unclear. We evaluated the hypothesis that changes in hepatic deiodinases result from repair-related activation of the Hedgehog pathway in stromal cells. We localized deiodinase expression, assessed changes during injury, and determined how targeted manipulation of Hedgehog signaling in stromal cells impacted hepatic deiodinase expression, TH content, and TH action in rodents. Humans with chronic liver disease were also studied. In healthy liver, hepatocytes strongly expressed D1 and stromal cells weakly expressed D3. During injury, hepatocyte expression of D1 decreased, whereas stromal expression of D3 increased, particularly in myofibroblasts. Conditionally disrupting Hedgehog signaling in myofibroblasts normalized deiodinase expression. Repair-related changes in deiodinases were accompanied by reduced hepatic TH content and TH-regulated gene expression. In patients, this was reflected by increased serum rT3. Moreover, the decreases in the free T3 to rT3 and free T4 to rT3 ratios distinguished advanced from mild fibrosis, even in individuals with similar serum levels of TSH and free T4. In conclusion, the Hedgehog-dependent changes in liver stromal cells drive repair-related changes in hepatic deiodinase expression that promote intrahepatic hypothyroidism, thereby limiting exposure to T3, an important factor for cellular differentiation.
Re-thinking the Concept of Surplus: Embracing Co-creation Experiences in Economics
Authors: Chakrabarti Avik , Ramaswamy Venkat 
Published: August 2014

Abstract
Economics has traditionally split consumers from producers by assigning them fixed roles. In the Internetworked economy as it is becoming, this is no longer true as value is increasingly jointly created among individuals, as a function of their co-creation experiences. We construct a framework that reconnects economics with value creation that goes beyond artifacts as the central unit of analysis, toward platforms of engagements of which artifacts themselves are only a part. Engagement platforms are now both the means and ends of value creation, and economic theory must take into account both the potential value generated to individuals through “production” experiences of co-creating artifacts (through co-production engagement platforms), as well as co-creating
“consumption” experiences (through co-consumption engagement platforms). We show how starting with joint value creation re-shapes our thinking about the nature of “surplus,” expanding traditional economic thinking about supply and demand toward a more convergent space of markets shaped by co-creation experiences.
Physical Properties of Red Guava (Psidium guajava L.) Pulp as Affected by Soluble Solids Content and Temperature
Authors: Diniz Renata Silva , Coimbra Jane Sélia dos Reis , Martins Marcio Arêdes , de Oliveira dos Santos Michel , Diniz Mayra Darliane Martins Silva , de Souza Santos Emílio , Santánna Danielle Dias , da Rocha Roney Alves , de Oliveira Eduardo Basílio 
Published: August 2014

Abstract
Physical properties of fluid and semisolid foods, such as density and rheological behavior, must be carefully taken into account on designing unit operations for the processing of such kind of products. In this work, a rotational rheometer of concentric cylinders was used to evaluate the rheological behavior of red guava pulp (Psidium guajava L.), with different soluble solids content (5, 10, and 15°Brix), at four temperatures (10, 30, 50, and 70°C). Also density data were obtained using pycnometry. Models were fitted to the obtained experimental data, in order to mathematically represent the rheological parameters and the density as functions of temperature and soluble solids content. The rheological behavior of the red guava pulp was adequately described by the Ostwald-de-Waele model, with a pseudoplastic behavior. Models to describe the simultaneous effect of temperature and concentration on the density were also presented.
L’ ECL. 1 E L’ ECL. 10 DI VIRGILIO: CONSIDERAZIONI SU UN RAPPORTO COMPLESSO
Authors: Gagliardi Paola 
Published: August 2014

Abstract
A comparison between ecl. 1 and ecl. 10 shows not only the deep differences of the new Virgilian pastoral poetry from Theocritus’ bucolic, but confirms that even Virgil lacked confidence in the power of poetry to solace real sorrow, an idea maintained throughout the whole Bucolicon liber. Interesting analogies between the first and the last eclogue help furthermore to explain Virgil’s farewell to his bucolic production.
ON THE GEOMETRICAL TERM RADIUS IN ANCIENT LATIN
Authors: Bohlin Erik 
Published: August 2014

Abstract
According to major Latin dictionaries, the word radius is attested as a terminus technicus for the geometrical concept ‘radius’ in Cicero’s Timaeus 17. In this study, however, it is argued that there is good reason to believe that Cicero did not use the word in this sense, but in a metaphorical expression in which radius mainly carries the well-attested sense of ‘rod (for drawing diagrams, etc.)’: paribus radiis attingi literally = ‘to be touched by equal rods’, that is to say, ‘to be equidistant’. The first and only convincing attestation of radius in the sense of ‘radius (of a circle, sphere, etc.)’ in ancient Latin literature is found in Calcidius’ Commentary
on the Timaeus 59. It is suggested that Calcidius was influenced by Cicero’s translation and consequently adopted the word radius; however, its technical sense of ‘radius’ was coined, deliberately or not, by Calcidius himself. As there are no other convincing attestations of this sense of the word in ancient Latin, not even in geometrical texts, it is suggested that the word radius never became a commonly accepted terminus technicus for radius in antiquity.
Genetic Interactions BetweenPElements Involved in piRNA-Mediated Repression of Hybrid Dysgenesis inDrosophila melanogaster
Authors: Simmons Michael J , Meeks Marshall W , Jessen Erik , Becker Jordan R , Buschette Jared T , Thorp Michael W 
Published: August 2014

AbstractPrevious studies have shown that telomeric P elements inserted at the left end of the X chromosome are anchors of the P cytotype, the maternally inherited state that regulates P-element activity in the germ line of Drosophila melanogaster. This regulation is mediated by small RNAs that associate with the Piwi family of proteins (piRNAs). We extend the analysis of cytotype regulation by studying new combinations of telomeric and nontelomeric P elements (TPs and non-TPs). TPs interact with each other to enhance cytotype regulation. This synergism involves a strictly maternal effect, called presetting, which is apparently mediated by piRNAs transmitted through the egg. Presetting by a maternal TP can elicit regulation by an inactive paternally inherited TP, possibly by stimulating its production of primary piRNAs. When one TP has come from a stock heterozygous for a mutation in the aubergine, piwi, or Suppressor of variegation 205 genes, the synergism between two TPs is impaired. TPs also interact with non-TPs to enhance cytotype regulation, even though the non-TPs lack regulatory ability on their own. Non-TPs are not susceptible to presetting by a TP, nor is a TP susceptible to presetting by a non-TP. The synergism between TPs and non-TPs is stronger when the TP was inherited maternally. This synergism may be due to the accumulation of secondary piRNAs created by ping-pong cycling between primary piRNAs from the TPs and mRNAs from the non-TPs. Maternal transmission of P-element piRNAs plays an important role in the maintenance of strong cytotype regulation over generations.
Construction of a Microsatellites-Based Linkage Map for the White Grouper (Epinephelus aeneus)
Authors: Dor Lior , Shirak Andrey , Gorshkov Sergei , Band Mark R , Korol Abraham , Ronin Yefim , Curzon Arie , Hulata Gideon , Seroussi Eyal , Ron Micha 
Published: August 2014

Abstract
The white grouper (Epinephelus aeneus) is a promising candidate for domestication and aquaculture due to its fast growth, excellent taste, and high market price. A linkage map is an essential framework for mapping quantitative trait loci for economic traits and the study of genome evolution. DNA of a single individual was deep-sequenced, and microsatellite markers were identified in 177 of the largest scaffolds of the sequence assembly. The success rate of developing polymorphic homologous markers was 94.9% compared with 63.1% of heterologous markers from other grouper species. Of the 12 adult mature fish present in the broodstock tank, two males and two females were identified as parents of the assigned offspring by parenthood analysis using 34 heterologous markers. A single full-sib family of 48 individuals was established for the construction of first-generation linkage maps based on genotyping data of 222 microsatellites. The markers were assigned to 24 linkage groups in accordance to the 24 chromosomal pairs. The female and male maps consisting of 203 and 202 markers spanned 1053 and 886 cM, with an average intermarker distance of 5.8 and 5.0 cM, respectively. Mapping of markers to linkage groups ends was enriched by using markers originating from scaffolds harboring telomeric repeat-containing RNA. Comparative mapping showed high synteny relationships among the white grouper, kelp grouper (E. bruneus), orange-spotted grouper (E. coioides), and Nile tilapia (Oreochromis niloticus). Thus, it would be useful to integrate the markers that were developed for different groupers, depending on sharing of sequence data, into a comprehensive consensus map.
Distinct and Predictive Histone Lysine Acetylation Patterns at Promoters, Enhancers, and Gene Bodies
Authors: Rajagopal Nisha , Ernst Jason , Ray Pradipta , Wu Jie , Zhang Michael , Kellis Manolis , Ren Bing 
Published: August 2014

Abstract
In eukaryotic cells, histone lysines are frequently acetylated. However, unlike modifications such as methylations, histone acetylation modifications are often considered redundant. As such, the functional roles of distinct histone acetylations are largely unexplored. We previously developed an algorithm RFECS to discover the most informative modifications associated with the classification or prediction of mammalian enhancers. Here, we used this tool to identify the modifications most predictive of promoters, enhancers, and gene bodies. Unexpectedly, we found that histone acetylation alone performs well in distinguishing these unique genomic regions. Further, we found the association of characteristic acetylation patterns with genic regions and association of chromatin state with splicing. Taken together, our work underscores the diverse functional roles of histone acetylation in gene regulation and provides several testable hypotheses to dissect these roles.
Effects of Scaling and Root Planing on Clinical Response and Serum Levels of Adipocytokines in Patients With Obesity and Chronic Periodontitis
Authors: Dias Gonçalves Tiago Eduardo , Feres Magda , Zimmermann Glaucia Santos , Faveri Marcelo , Figueiredo Luciene Cristina , Braga Paloma Gralha , Duarte Poliana Mendes 
Published: August 2014

Background: Despite several investigations suggesting that obesity is a risk indicator for periodontitis, little is known about the effect of obesity on periodontal treatment response. The aim of this study is to evaluate the effects of scaling and root planing (SRP) on clinical parameters and circulating levels of leptin and adiponectin in patients with obesity with chronic periodontitis (CP).Methods: Twenty‐four patients with obesity and CP and 24 patients without obesity with CP were submitted to SRP. Clinical parameters were assessed at baseline and 3 and 6 months after therapy. Serum levels of leptin and adiponectin were evaluated at all time points, using enzyme‐linked immunosorbent assay.Results: SRP improved the clinical parameters of both groups at 3 and 6 months (P <0.05). Nonetheless, the patients without obesity presented a lower mean probing depth (PD) at 6 months after therapy and a greater reduction in PD from baseline to 6 months in the full‐mouth analysis (primary outcome variable) and in initially deep sites (P <0.05). Leptin serum levels were higher in patients with obesity than in patients without obesity at all time points (P <0.05). No changes in the serum levels of leptin and adiponectin were observed in groups with and without obesity after therapy (P >0.05).Conclusions: Patients with obesity and CP presented lower reductions in PD than patients without obesity with CP at 6 months after SRP. Furthermore, the treatment did not affect the circulating levels of leptin and adiponectin in any group.