bluuebunny/crossref_metadata_2025_split huggingface dataset:
Capturing the Experience
Authors: Gripsrud Birgitta Haga , Brassil Kelly J. , Summers Barbara , Søiland Håvard , Kronowitz Steven , Lode Kirsten 
Published: September 2015

Background:
Expressive writing has been shown to improve quality of life, fatigue, and posttraumatic stress among breast cancer patients across cultures. Understanding how and why the method may be beneficial to patients can increase awareness of the psychosocial impact of breast cancer and enhance interventional work within this population. Qualitative research on experiential aspects of interventions may inform the theoretical understanding and generate hypotheses for future studies.

Aim:
The aim of the study was to explore and describe the experience and feasibility of expressive writing among women with breast cancer following mastectomy and immediate or delayed reconstructive surgery.

Methods:
Seven participants enrolled to undertake 4 episodes of expressive writing at home, with semistructured interviews conducted afterward and analyzed using experiential thematic analysis.

Results:
Three themes emerged through analysis: writing as process, writing as therapeutic, and writing as a means to help others.

Conclusions:
Findings illuminate experiential variations in expressive writing and how storytelling encourages a release of cognitive and emotional strains, surrendering these to reside in the text. The method was said to process feelings and capture experiences tied to a new and overwhelming illness situation, as impressions became expressions through writing. Expressive writing, therefore, is a valuable tool for healthcare providers to introduce into the plan of care for patients with breast cancer and potentially other cancer patient groups.

Implications for Practice:
This study augments existing evidence to support the appropriateness of expressive writing as an intervention after a breast cancer diagnosis. Further studies should evaluate its feasibility at different time points in survivorship.
Back to School After Cancer Treatment
Authors: Choquette Anne , Rennick Janet E. , Lee Virginia 
Published: September 2015

Background:
Little is known about the influence of cancer on the adolescent’s developing self-identity and social relationships as he/she transitions back to school following cancer treatment.

Objective:
The purpose of this study was to explore the meaning of returning to school for adolescents who have completed cancer treatment.

Methods:
In this qualitative study, in-depth interviews using an interpretive descriptive approach were conducted with 11 adolescents (aged 13–17 years) who had completed treatment for cancer. The transcripts of the audiotaped interviews were analyzed thematically and reviewed by the study team.

Results:
Three main themes suggested that the return to school hallmarked the end of an illness episode and a welcome return to a sense of well-being: (a) being on the right track, (b) bridging two worlds, and (c) establishing a new life at school. Nearly all adolescents described being negatively impacted by the cancer experience. However, the ability to maintain friendships during the transition emerged as particularly salient to allow the adolescents to rise above the challenges and residual effects of cancer treatment.

Conclusions:
Returning to school following cancer treatment affects adolescents’ beliefs about themselves, their self-identity, and their social relationships. Understanding the meaning that adolescents ascribed to returning to school facilitated the development of practice recommendations to improve adjustment to school.

Implications for Practice:
Our study findings illuminate an important gap in the existing resources for adolescents in the posttreatment phase of cancer. Recommendations to promote healthy psychosocial development are proposed to better support adolescents during the reintegration to school.
Pilot Randomized Controlled Trial of Auricular Point Acupressure to Manage Symptom Clusters of Pain, Fatigue, and Disturbed Sleep in Breast Cancer Patients
Authors: Yeh Chao Hsing , Chien Lung-Chang , Lin Wei-Chun , Bovbjerg Dana Howard , van Londen GJ 
Published: September 2015

Background:
Current management for a symptom cluster of pain, fatigue, and disturbed sleep in breast cancer patients has limited effects.

Objective:
The purposes of this prospective, randomized controlled pilot study were to (1) assess the feasibility and tolerability of auricular point acupressure (APA) intervention to manage pain, fatigue, and sleep disturbance in breast cancer patients and (2) provide an initial appraisal of effect size as compared with a control intervention.

Methods:
Thirty-one participants were randomized into either an active APA group (n = 16) or a control APA group (n = 15), which included the sham APA treatment not related to the symptoms. All participants received the APA once a week for 4 weeks. Self-report measures were obtained at baseline, weekly during intervention, at end of intervention, and at a 1-month follow-up.

Results:
For the 4-week of APA treatment, the retention rate was 88% for the active APA group and 73% for the control APA group. After 4 weeks of APA, participants in the active APA treatment had reported a reduction of 71% in pain, 44% in fatigue, 31% in sleep disturbance, and 61% in interference with daily activities. The control APA group experienced some moderate reduction in these symptoms.

Conclusion:
Given that this was a pilot study with a small sample size, results must be interpreted with caution.

Implications for Practice:
Our results suggest that APA may provide an inexpensive and effective complementary approach for the management of symptom clusters for breast cancer patients, and further study is warranted.
WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
Authors: Wong Nathan , Liu Weijun , Wang Xiaowei 
Published: September 2015

AbstractThe CRISPR/Cas9 system has been rapidly adopted for genome editing. However, one major issue with this system is the lack of robust bioinformatics tools for design of single guide RNA (sgRNA), which determines the efficacy and specificity of genome editing. To address this pressing need, we analyze CRISPR RNA-seq data and identify many novel features that are characteristic of highly potent sgRNAs. These features are used to develop a bioinformatics tool for genome-wide design of sgRNAs with improved efficiency. These sgRNAs as well as the design tool are freely accessible via a web server, WU-CRISPR (http://crispr.wustl.edu).
ZNF191 Inhibits Hepatocellular Carcinoma Metastasis via DLG1-mediated YAP1 Inactivation
Authors: Wu Di , Li Haitao , Liu Yufeng , Saiyin Hexige , Wang Chenji , Wei Zhen , Zen Wenjiao , Liu Danyang , Chen Qi , Huang Haojie , Liu Guoyuan , Jiang Songmin , Yu Long 
Published: September 2015

AbstractSearching targets for hepatocellular carcinoma (HCC) treatment, we identified zinc finger protein 191 (ZNF191) as a suppressor against HCC metastasis. Over-expressing ZNF191 in HCC cells impaired cell motility, while ZNF191 depletion promoted HCC cell migrationin vitroand metastasisin vivothrough triggering yes-associated protein 1 (YAP1) signaling. Chromatin immunoprecipitation-sequencing (ChIP-seq) revealed that ZNF191 specifically bound to the promoter ofDiscs, Large homolog 1(DLG1), a cell polarity maintainer and a negative regulator of YAP1. Double-knockdown experiments showed that DLG1 was not only the mediator of ZNF191’s function to suppress migration but also a link between ZNF191 and YAP1 signaling. ZNF191 was down-regulated in metastatic HCCs, correlating positively with DLG1 levels and inversely with YAP1 activation. Our findings indicate ZNF191 functions as a metastasis suppressor via DLG1-mediated YAP1 signaling inactivation.
The potential of electricity transmission corridors in forested areas as bumble bee habitat
Authors: Hill Bruce , Bartomeus Ignasi 
Published: September 2015

AbstractDeclines in pollinator abundance and diversity are not only a conservation issue but also a threat to crop pollination. Maintained infrastructure corridors, such as those containing electricity transmission lines, are potentially important wild pollinator habitat. However, there is a lack of evidence comparing the abundance and diversity of wild pollinators in transmission corridors with other important pollinator habitats. We compared the diversity of a key pollinator group, bumble bees (Bombus spp.), between transmission corridors and the surrounding semi-natural and managed habitat types at ten sites across Sweden’s Uppland region. Our results show that transmission corridors have no impact on bumble bee diversity in the surrounding area. However, transmission corridors and other maintained habitats have a level of bumble bees abundance and diversity comparable to semi-natural grasslands and host species that are important for conservation and ecosystem service provision. Under the current management regime, transmission corridors already provide valuable bumble bee habitat, but given that host plant density is the main determinant of bumble bee abundance, these areas could potentially be enhanced by establishing and maintaining key host plants. We show that in northern temperate regions the maintenance of transmission corridors has the potential to contribute to bumble bee conservation and the ecosystem services they provide.
Simultaneous enhancement of multiple stability properties using two-parameter control methods inDrosophila melanogaster
Authors: Tung Sudipta , Mishra Abhishek , Dey Sutirth 
Published: September 2015

AbstractAlthough a large number of methods have been proposed to control the non-linear dynamics of unstable popuations, very few have been actually adopted for application. One reason for this gap is the fact that few control methods have been empirically verified using biological populations. To address this issue, we investigated the effects of two well-studied control methods (Both Limiter Control and Target-Oriented Control) on the dynamics of unstable populations ofDrosophila melanogaster. We show that both methods can significantly reduce population fluctuations, decrease extinction probability and increase effective population size simultaneously. This is in contrast with single parameter control methods that are not able to achieve multiple aspects of stability at the same time. We use the distribution of population sizes to derive biologically intuitive explanations for the mechanisms of how these two control methods attain stability. Finally, we show that non-Drosophila specific biologically realistic simulations are able to capture almost all the trends of our data. This shows that our results are likely generalizable over a wide range of taxa. The primary insight of our study is that control methods that incorporate both culling and restocking have better all-round performance in terms of stabilizing populations.
MTG2: An efficient algorithm for multivariate linear mixed model analysis based on genomic information
Authors: Lee S.H. , van der Werf J.H.J. 
Published: September 2015

AbstractWe have developed an algorithm for genetic analysis of complex traits using genome-wide SNPs in a linear mixed model framework. Compared to current standard REML software based on the mixed model equation, our method could be more than 1000 times faster. The advantage is largest when there is only a single genetic covariance structure. The method is particularly useful for multivariate analysis, including multitrait models and random regression models for studying reaction norms. We applied our proposed method to publicly available mice and human data and discuss advantages and limitations.Availability:MTG2 is available inhttps://sites.google.com/site/honglee0707/mtg2.Contact:hong.lee@une.edu.auSupplementary information:Supplementary data are available.
Laplacian eigenmaps and principal curves for high resolution pseudotemporal ordering of single-cell RNA-seq profiles
Authors: Campbell Kieran , Ponting Chris P , Webber Caleb 
Published: September 2015

AbstractAdvances in RNA-seq technologies provide unprecedented insight into the variability and heterogeneity of gene expression at the single-cell level. However, such data offers only a snapshot of the transcriptome, whereas it is often the progression of cells through dynamic biological processes that is of interest. As a result, one outstanding challenge is to infer such progressions by ordering gene expression from single cell data alone, known as the cell ordering problem. Here, we introduce a new method that constructs a low-dimensional non-linear embedding of the data using laplacian eigenmaps before assigning each cell a pseudotime using principal curves. We characterise why on a theoretical level our method is more robust to the high levels of noise typical of single-cell RNA-seq data before demonstrating its utility on two existing datasets of differentiating cells.
Antiarrhythmic activity in occlusion‐reperfusion model of 1‐(1H‐indol‐4‐yloxy)‐3‐{[2‐(2‐methoxyphenoxy)ethyl]amino} propan‐2‐ol and its enantiomers
Authors: Bednarski Marek , Otto Monika , Dudek Magdalena , Siwek Agata , Zygmunt Małgorzata , Knutelska Joanna , Nowiński Leszek , Groszek Grażyna , Sapa Jacek 
Published: September 2015

SummaryAcute myocardial infarction (AMI) is a leading cause of mortality and morbidity worldwide, especially in developed countries. The most serious problem after myocardial infarction is reperfusion injury that manifests as functional impairment, arrhythmia, and accelerated progression of cell death in certain critically injured myocytes. Subsequently the infarcted myocardium develops features of necrosis and reactive inflammation. To reduce lethal reperfusion injury in patient with AMI antioxidants, anti‐inflammatory agents, adenosine, opioids, metabolic modulators (glucose, insulin, and potassium, nicorandil and agents which reduce intracellular Ca2+ overload and inhibit Na+‐H+ exchange) are used. In this study a novel compound (compound 9) 1‐(1 h‐indol‐4‐yloxy)‐3‐{[2‐(2‐methoxyphenoxy) ethyl]amino}propan‐2‐ol and its enantiomers are examined in arrhythmia associated with coronary artery occlusion and reperfusion in a rat model. Antioxidant properties are also determined for test compounds using the malondialdehyde (MDA) lipid peroxidation and ferric reducing antioxidant power (FRAP) tests. In summary, the tested compounds, especially the S enantiomer has a strong antiarrhythmic activity in a model of occlusion and reperfusion of the left coronary artery which is probably related to their adrenolytic action. In contrast to carvedilol, none of the test compound reduced the lipid peroxidation but increased ferric reducing antioxidant power. In the antioxidant effect, there was no difference between the optical forms of compound 9.
Oxygen‐glucose deprivation preconditioning protects neurons against oxygen‐glucose deprivation/reperfusion induced injury via bone morphogenetic protein‐7 mediated ERK, p38 and Smad signalling pathways
Authors: Guan Junhong , Du Shaonan , Lv Tao , Qu Shengtao , Fu Qiang , Yuan Ye 
Published: September 2015

SummaryBone morphogenetic protein (BMP)‐7 mediated neuroprotective effect of cerebral ischemic preconditioning (IPC) has been studied in an ischemic animal model, but the underlying cellular mechanisms have not been clearly clarified. In this study, primary cortical neurons and the SH‐SY5Y cell line were used to investigate the role of BMP‐7 and its downstream signals in the neuroprotective effects of oxygen‐glucose deprivation preconditioning (OGDPC). Immunocytochemistry was used to detect the expression of neurofilament in neurons. MTT and lactate dehydrogenase activity assays were used to measure the cytotoxicity. Western blot was used to detect the protein expression of BMP‐7 and downstream signals. BMP inhibitor, mitogen‐activated protein kinase inhibitors, Smad inhibitor and siRNA of Smad 1 were used to investigate the role of corresponding signalling pathways in the OGDPC. Results showed that OGDPC‐induced overexpression of BMP‐7 in primary cortical neurons and SH‐SY5Y cells. Both of endogenous and exogenous BMP‐7 could replicate the neuroprotective effects seen in OGDPC pretreatment. In addition, extracellular regulated protein kinases, p38 and Smad signalling pathway were found to be involved in the neuroprotective effects mediated by OGDPC via BMP‐7. This study primarily reveals the cellular mechanisms of the neuroprotection mediated by OGDPC, and provides evidence for better understanding of this intrinsic factor against ischemia.
Tobacco denormalisation and representations of different tobacco users in Norway: a cross‐sectional study
Authors: Sæbø Gunnar 
Published: September 2015

AbstractThis study asks whether representations of different typical tobacco users vary in line with established stereotypes and by social group membership (especially tobacco user status). Social identity theory posits that representations differ due to varying identification with product users on the basis of personal use, while distinction theory suggests representations will be structured by proximity and distance to user groups. Using principal component analysis to identify representations and regression analysis to determine variances in representations, we find that four of five groups of typical tobacco users (snus users, pipe, cigarette, and cigar smokers) can be classified according to three similar representations: socially secure, culturally marginal and unappealing. Respondents who themselves use a certain tobacco product are more likely to consider a ‘typical’ user from their own group as more ‘socially secure’ and less ‘culturally marginal’ and ‘unappealing’ than non‐users. Former smokers tend to side with non‐smokers, which suggests their ‘smoker identity’ is on the wane. At the representational level, then, socio‐historical distinctions between different tobacco products seem to have diminished in favour of a largely dual classification, with users expressing positive and non‐users negative representations, of any typical user. This is a likely consequence of persistent tobacco denormalisation policies.
Clinical Effects of a Longer Duration of Polymyxin B‐Immobilized Fiber Column Direct Hemoperfusion Therapy for Severe Sepsis and Septic Shock
Authors: Yamashita Chizuru , Hara Yoshitaka , Kuriyama Naohide , Nakamura Tomoyuki , Nishida Osamu 
Published: September 2015

AbstractPolymyxin B‐immobilized fiber column direct hemoperfusion (PMX‐DHP) therapy is widely used for the treatment of severe sepsis and septic shock, and is generally performed for 2 h. Although previous studies demonstrated the efficacy of PMX‐DHP therapy, it currently remains unclear whether its optimal duration is 2 h. This retrospective study analyzed 37 patients with septic shock who showed a poor clinical response to 2 h of PMX‐DHP, and underwent a longer duration of this therapy. The mean duration of PMX‐DHP therapy was 15.8 ± 7.9 h, and none of the patients developed adverse events, which enabled the therapy to be performed safely. The pressure catecholamine index [CAIP = catecholamine index/mean arterial pressure; catecholamine index = dopamine + dobutamine + (adrenaline + noradrenaline) × 100 μg/kg per min], as an indicator of hemodynamics, improved significantly in the survival group in the period between the start and 24 h after the end of PMX‐DHP therapy (P < 0.01), and between 2 h after the start of and the end of this therapy (P < 0.05). In addition, the P/F ratio improved significantly in the group of surviving patients with acute respiratory distress syndrome (ARDS) in the period between the start and 24 h after the end of PMX‐DHP therapy (P < 0.01), and between 2 h after the start of and the end of this therapy (P < 0.01). These results suggest that a longer duration of PMX‐DHP therapy can be expected to improve the hemodynamics and pulmonary oxygenation capacity of patients with severe sepsis/septic shock. Strict prospective studies are needed in the future.
Granulocyte and Monocyte Adsorption Apheresis for Generalized Pustular Psoriasis: Therapeutic Outcomes in Three Refractory Patients
Authors: Fujisawa Tomomi , Suzuki Satoko , Mizutani Yoko , Doi Tomoaki , Yoshida Shozo , Ogura Shinji , Seishima Mariko 
Published: September 2015

AbstractGeneralized pustular psoriasis (GPP) is a type of neutrophilic dermatosis that is sometimes resistant to medications. In patients with neutrophilic skin diseases, granulocyte and monocyte adsorption apheresis (GMA) has been demonstrated to selectively and efficiently eliminate myeloid‐lineage leukocytes from the peripheral blood. We evaluated the efficacy and safety of repeated GMA therapy in three refractory GPP patients. Three GPP patients refractory to previous therapies received weekly GMA with five sessions per course, which was repeated when the symptoms reappeared. The efficacy was assessed by the disease severity scores 2 weeks after each course of GMA. The GPP severity scores of all three patients were reduced in all courses (N = 9); they were reduced by more than 3 points in six courses and by 2 points in three courses. After the first GMA course, the GPP severity scores were reduced by more than 3 points in all three patients. On average, the GPP severity scores were reduced by 4.67 and 3.67 points after the first course and repeated courses, respectively. The severity of edema and pustules were particularly improved in all patients and no adverse effects were observed. GMA showed efficacy for the treatment of refractory GPP patients as a non‐pharmacologic intervention without any associated adverse effects, and was particularly effective in the first course, but also effective in the subsequent courses.
Plasma Adiponectin Levels in Acute Liver Failure Patients Treated with Plasma Filtration with Dialysis and Plasma Exchange
Authors: Yamamoto Hiroshi , Nakae Hajime , Uji Yoshitaka , Maeda Kazuhisa , Tani Tohru , Eguchi Yutaka 
Published: September 2015

AbstractPlasma filtration with dialysis (PDF) is a blood purification therapy in which simple plasma exchange (PE) is performed using a selective membrane plasma separator while the dialysate flows outside of the hollow fibers. Improvement of hypoadiponectinemia is considered to be a useful therapeutic approach for ameliorating fatal conditions including cardio‐metabolic and infectious disease. We investigated the effects of PDF in comparison to PE in terms of plasma adiponectin (APN) changes in patients with acute liver failure. Seventeen patients with liver failure were studied; PDF was performed 55 times and PE 14 times. Plasma APN levels increased significantly after PDF, while decreasing significantly after PE. PDF appears to be among the most useful blood purification therapies in acute liver failure cases in terms of increasing APN levels.