bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of oleic acid as co-substrate of glucose on “short” and “long-term” Crabtree effect in Saccharomyces cerevisiae
Authors: Marc Jillian , Feria-Gervasio David , Mouret Jean-Roch , Guillouet Stéphane E 
Published: September 2013

Abstract

Background
Optimization of industrial biomass directed processes requires the highest biomass yield as possible. Yet, some useful yeasts like Saccharomyces cerevisiae are subject to the Crabtree effect under glucose excess. This phenomenon can occur in large scale tank where heterogeneities in glucose concentrations exist. Therefore yeasts encounter local environments with glucose excess leading to ethanol production to the detriment of biomass formation. We previously demonstrated that oleic acid as a co-substrate in glucose-limited chemostat allowed to delay and modulate the “short-term” Crabtree effect in Saccharomyces cerevisiae. Here we further investigated the effect of oleic acid as a modulator of the Crabtree effect.

Results
The impact of oleic acid as co-substrate on the Crabtree effect was investigated in terms of i) strain specificity, ii) reversibility of the potential effect with aerobic glucose-excess batches and iii) durability and maximal capacities under high ethanol stress with glucose-excess fed-batches. First, the addition of oleic acid resulted in an increase of the critical dilution rate by 8% and the specific carbon uptake rate by 18%. Furthermore, a delay was observed for the onset of ethanol production when a batch was inoculated with cells previously grown in glucose-oleate chemostat. Finally, the culture of adapted cells in a glucose-oleate fed-batch led to a redirection of the carbon flux toward biomass production, with a 73% increase in the biomass yield.

Conclusions
This work demonstrated clearly that the perturbation by oleic acid as co-substrate resulted in a decrease in the “short-term” and “long-term” Crabtree effects. This impact was not strain dependent and reversible. Thus, industrial applications of this biochemical strategy could be envisaged to tackle heterogeneities issues in large scale tanks or to prepare starter yeasts for various applications.
Multivariate matching pursuit in optimal Gabor dictionaries: theory and software with interface for EEG/MEG via Svarog
Authors: Kuś Rafał , Różański Piotr Tadeusz , Durka Piotr Jerzy 
Published: September 2013

Abstract

Background
Matching pursuit algorithm (MP), especially with recent multivariate extensions, offers unique advantages in analysis of EEG and MEG.

Methods
We propose a novel construction of an optimal Gabor dictionary, based upon the metrics introduced in this paper. We implement this construction in a freely available software for MP decomposition of multivariate time series, with a user friendly interface via the Svarog package (Signal Viewer, Analyzer and Recorder On GPL, http://braintech.pl/svarog), and provide a hands-on introduction to its application to EEG. Finally, we describe numerical and mathematical optimizations used in this implementation.

Results
Optimal Gabor dictionaries, based on the metric introduced in this paper, for the first time allowed for a priori assessment of maximum one-step error of the MP algorithm. Variants of multivariate MP, implemented in the accompanying software, are organized according to the mathematical properties of the algorithms, relevant in the light of EEG/MEG analysis. Some of these variants have been successfully applied to both multichannel and multitrial EEG and MEG in previous studies, improving preprocessing for EEG/MEG inverse solutions and parameterization of evoked potentials in single trials; we mention also ongoing work and possible novel applications.

Conclusions
Mathematical results presented in this paper improve our understanding of the basics of the MP algorithm. Simple introduction of its properties and advantages, together with the accompanying stable and user-friendly Open Source software package, pave the way for a widespread and reproducible analysis of multivariate EEG and MEG time series and novel applications, while retaining a high degree of compatibility with the traditional, visual analysis of EEG.
Knockdown of the C. elegans Kinome identifies Kinases required for normal protein Homeostasis, Mitochondrial network structure, and Sarcomere structure in muscle
Authors: Lehmann Susann , Bass Joseph J , Szewczyk Nathaniel J 
Published: September 2013

Abstract

Background
Kinases are important signalling molecules for modulating cellular processes and major targets of drug discovery programs. However, functional information for roughly half the human kinome is lacking. We conducted three kinome wide, >90%, RNAi screens and epistasis testing of some identified kinases against known intramuscular signalling systems to increase the functional annotation of the C. elegans kinome and expand our understanding of kinome influence upon muscle protein degradation.

Results
96 kinases were identified as required for normal protein homeostasis, 74 for normal mitochondrial networks and 50 for normal sarcomere structure. Knockdown of kinases required only for normal protein homeostasis and/or mitochondrial structure was significantly less likely to produce a developmental or behavioural phenotype than knockdown of kinases required for normal sarcomere structure and/or other sub-cellular processes. Lastly, assessment of kinases for which knockdown produced muscle protein degradation against the known regulatory pathways in C. elegans muscle revealed that close to half of kinase knockdowns activated autophagy in a MAPK dependent fashion.

Conclusions
Roughly 40% of kinases studied, 159 of 397, are important in establishing or maintaining muscle cell health, with most required for both. For kinases where decreased expression triggers protein degradation, autophagy is most commonly activated. These results increase the annotation of the C. elegans kinome to roughly 75% and enable future kinome research. As 33% of kinases identified have orthologues expressed in human muscle, our results also enable testing of whether identified kinases function similarly in maintaining human muscle homeostasis.
Plasma microRNA-133a is a new marker for both acute myocardial infarction and underlying coronary artery stenosis
Authors: Wang Feng , Long Guangwen , Zhao Chunxia , Li Huaping , Chaugai Sandip , Wang Yan , Chen Chen , Wang Dao Wen 
Published: September 2013

Abstract

Background
Previous study demonstrated that miR-133a was released into blood from injured myocardium in cardiovascular diseases. However, the dynamic change of circulating miR-133a level in the early phase of acute myocardial infarction (AMI) and the correlation between miR-133a and severity of coronary stenosis in coronary heart disease (CHD) patients are not clear.

Methods and results
Three different cohorts (including 13 AMI patients, 176 angina pectoris patients and 127 control subjects) were enrolled to investigate the expression levels of circulating miR-133a in patients with myocardial ischemia and also the relationship between plasma miR-133a and severity of coronary stenosis. Plasma miR-133a levels of participants were examined by real-time quantitative PCR. Simultaneously, plasma cardiac troponin I (cTnI) concentrations were measured by ELISA assays. The results showed that circulating miR-133a level was significantly increased in AMI patients in time-dependent manner, and achieved a 72.1 fold peak at 21.6 ± 4.5 hours after the onset of AMI symptoms and exhibited a similar trend to plasma cTnI level. We also found that plasma miR-133a levels were higher in CHD patients than control group. Importantly, the levels of circulating miR-133a positively correlated with the severities of the coronary artery stenosis. Receiver operating characteristic (ROC) analysis revealed that circulating miR-133a had considerable diagnostic accuracy for CHD with an AUC of 0.918 (95% confidence interval 0.877-0.960).

Conclusions
Circulating miR-133a may be a new biomarker for AMI and as a potential diagnostic tool. And increased miR-133a level may be used to predict both the presence and severity of coronary lesions in CHD patients.
Acute and chronic nephrotoxicity of platinum nanoparticles in mice
Authors: Yamagishi Yoshiaki , Watari Akihiro , Hayata Yuya , Li Xiangru , Kondoh Masuo , Yoshioka Yasuo , Tsutsumi Yasuo , Yagi Kiyohito 
Published: September 2013

Abstract
Platinum nanoparticles are being utilized in various industrial applications, including in catalysis, cosmetics, and dietary supplements. Although reducing the size of the nanoparticles improves the physicochemical properties and provides useful performance characteristics, the safety of the material remains a major concern. The aim of the present study was to evaluate the biological effects of platinum particles less than 1 nm in size (snPt1). In mice administered with a single intravenous dose of snPt1, histological analysis revealed necrosis of tubular epithelial cells and urinary casts in the kidney, without obvious toxic effects in the lung, spleen, and heart. These mice exhibited dose-dependent elevation of blood urea nitrogen, an indicator of kidney damage. Direct application of snPt1 to in vitro cultures of renal cells induced significant cytotoxicity. In mice administered for 4 weeks with twice-weekly intraperitoneal snPt1, histological analysis of the kidney revealed urinary casts, tubular atrophy, and inflammatory cell accumulation. Notably, these toxic effects were not observed in mice injected with 8-nm platinum particles, either by single- or multiple-dose administration. Our findings suggest that exposure to platinum particles of less than 1 nm in size may induce nephrotoxicity and disrupt some kidney functions. However, this toxicity may be reduced by increasing the nanoparticle size.
Bernoulli numbers and certain convolution sums with divisor functions
Authors: Kim Daeyeoul , Kim Aeran , Yildiz Ikikardes Nazli 
Published: September 2013

Abstract
In this paper, we investigate the convolution sums

∑

(
a
+
b
+
c
)
x
=
n

a
,

∑

a
x
+
b
y
=
n

a
b
,

∑

a
x
+
b
y
+
c
z
=
n

a
b
c
,

∑

a
x
+
b
y
+
c
z
+
d
u
=
n

a
b
c
d
,

where

a
,
b
,
c
,
d
,
x
,
y
,
z
,
u
,
n
∈
N

. Many new equalities and inequalities involving convolution sums, Bernoulli numbers and divisor functions have also been given.

MSC:11A05, 33E99.
Supporting visual quality assessment with machine learning
Authors: Gastaldo Paolo , Zunino Rodolfo , Redi Judith 
Published: September 2013

Abstract
Objective metrics for visual quality assessment often base their reliability on the explicit modeling of the highly non-linear behavior of human perception; as a result, they may be complex and computationally expensive. Conversely, machine learning (ML) paradigms allow to tackle the quality assessment task from a different perspective, as the eventual goal is to mimic quality perception instead of designing an explicit model the human visual system. Several studies already proved the ability of ML-based approaches to address visual quality assessment; nevertheless, these paradigms are highly prone to overfitting, and their overall reliability may be questionable. In fact, a prerequisite for successfully using ML in modeling perceptual mechanisms is a profound understanding of the advantages and limitations that characterize learning machines. This paper illustrates and exemplifies the good practices to be followed.
Cervical necrosis after chemoradiation for cervical cancer: case series and literature review
Authors: Fawaz Ziad Simon , Barkati Maroie , Beauchemin Marie-Claude , Sauthier Philippe , Gauthier Philippe , Nguyen Thu Van 
Published: September 2013

Abstract

Background
The aim of this study was to assess the management of cervical necrosis (CN) following radiotherapy (RT) and the impact of smoking status. This rare complication mimics a neoplastic recurrence, and causes concern among attending physicians.

Methods
Between July 2008 and March 2013, 5 women on 285 with localized cervical cancer had a CN following RT. Patients were treated with concomitant chemoradiation. The medical records were reviewed to abstract demographic and clinical information until March 2013.

Results
1.75% (95% confidence interval: 0.23 to 3.28%) developed CN. All patients were smokers with a mean of 19.5 pack-years (range: 7.5-45 pack-years). All patients were treated with weekly Cisplatin chemotherapy and external beam radiation to the pelvis, 45 Gy in 25 fractions. Four patients received an extra boost with a median dose of 7.2 Gy (range: 5.4-10 Gy). All patients had intracavitary brachytherapy (range: 27.9 to 30 Gy). Clinical presentation was similar for all the cases: vaginal discharge associated with pain. Mean time for time post-radiation therapy to necrosis was 9.3 months (range: 2.2-20.5 months). Standard workup was done to exclude cancer recurrence: biopsies and radiologic imaging. Conservative treatment was performed with excellent results. Resolution of the necrosis was complete after a few months (range: 1 to 4 months). Median follow-up until March 2013 was 19 months. All the patients were alive with no clinical evidence of disease.

Conclusions
This study, the largest to date, shows that conservative management of CN after RT is effective, and should be attempted. This complication is more common in smokers, and counseling intervention should result in fewer complications of CN.
Identification of a Histidine Metal Ligand in the argE-Encoded N-Acetyl-L-Ornithine Deacetylase from Escherichia coli
Authors: McGregor Wade C , Gillner Danuta M , Swierczek Sabina I , Liu Dali , Holz Richard C 
Published: September 2013

Abstract
The H355A, H355K, H80A, and H80K mutant enzymes of the argE-encoded N-acetyl-L-ornithine deacetylase (ArgE) from Escherichia coli were prepared, however, only the H355A enzyme was found to be soluble. Kinetic analysis of the Co(II)-loaded H355A exhibited activity levels that were 380-fold less than Co(II)-loaded WT ArgE. Electronic absorption spectra of Co(II)-loaded H355A-ArgE indicate that the bound Co(II) ion resides in a distorted, five-coordinate environment and Isothermal Titration Calorimetry (ITC) data for Zn(II) binding to the H355A enzyme provided a dissociation constant (K
d) of 39 μM. A three-dimensional homology model of ArgE was generated using the X-ray crystal structure of the dapE-encoded N-succinyl-L,L-diaminopimelic acid desuccinylase (DapE) from Haemophilus influenzae confirming the assignment of H355 as well as H80 as active site ligands.
Die LaVaCro-Studie: Langzeitergebnisse der Varizenoperation mit Crossektomie und Stripping der V. saphena magna
Authors: Altenkämper H. , Bernheim C. , Broermann M. , Dresler C. , Frings N. , Hartmann M. , Heisterkamp T. , Jünger M. , Steffen H. P. , Stenger D. , Temboulatov M. , Mumme A. , Flessenkämper I. , Papapostolou G. 
Published: September 2013

ZusammenfassungHintergrund: Multizentrische Langzeitstudien mit hohen Fallzahlen nach Crossektomie und Stripping der Vena saphena magna (VSM) liegen nicht vor. Den in mehreren Studien und Registern erhobenen Daten zu den Rezidivraten der endovenösen Therapieverfahren stehen außerordentlich heterogene Daten der operativen Therapie gegenüber.Methode: In einer von der Arbeitsgemeinschaft für Venenoperationen (VOP-AG) der Deutschen Gesellschaft für Phlebologie (DGP) initiierten prospektiven Multizenterstudie wurden in 12 Zentren 841 Patienten (1 070 Extremitäten) mit einer Stammvarikose der VSM aufgenommen und entsprechend einem streng standardisierten Protokoll operiert. Primärer Endpunkt ist das hämodynamisch relevante saphenofemorale Rezidiv, definiert als eine Vene in der Crossenregion (Durchmesser >5 mm, Reflux >0,5 sec) mit einer Verbindung zur V. femoralis communis Sekundäre Endpunkte sind die Rate duplexsonografisch nachweisbarer pathologischer Crossenrefluxe (Durchmesser <5 mm, Reflux >0,5 sec.), perioperative Komplikationen und neu aufgetretene Varizen nach dem REVAS Klassifikation. Die klinischen und duplexsonografischen Kontrollen erfolgten 7 bis 14 Tage, 3 bis 4 Monate und ein Jahr postoperativ. Hiernach sind jährliche Kontrollen vorgesehen.Ergebnis: Die Rate klinischer Rezidive lag 3–4 Monate postoperativ bei 1,1 % und ein Jahr postoperativ bei 6,4 %. Die Rate duplexsonografischer Rezidive im Crossenbereich betrug nach 3–4 Monaten 0,53 % und nach einem Jahr 2,24 %. Davon waren lediglich zwei (0,29 %) ein Jahr postoperativ hämodynamisch relevant (Durchmesser >5 mm).Schlussfolgerung: Die unter standardisierten Bedingungen vorgenommene Crossektomie und Stripping-Operation weist im Verlauf bis zu einem Jahr eine geringe Rezidivrate auf.
E-survey on venous leg ulcer among Dutch dermatologists
Authors: de Roos K-P. , Vogels R. J. M. , Neumann H. A. M. , Reeder S. W. I. 
Published: September 2013

SummaryAim: To get insight into the frequency of venous leg ulceration in the Dutch dermato-logic practice, and into how this profession treats this disease.Design:Material and Methods: An e-survey was conducted. To all Dutch dermatologists and residents dermatology an email was sent with an online link to a questionnaire on venous leg ulcers.Results: The overall response was 30 %. 83.5 % of the doctors usually treat their patients according to the guideline. The dermatologic practice consists on average of 73 patients (range 0-500; SD 93) with leg ulceration, and yearly 54 new leg ulcer patients (range 0-300; SD 50) are seen. 65 % of the patients are women, 80 % is more than 45 years of age and 55 % is older than 65. Of all ulcers, 77 % has a venous aetiology, of which 59 % has a primary cause. Mean time to heal is 74 days (range 4-200; SD 39). Per year, dermatologists admit on average 7 patients (range 0-50; SD 11) because of leg ulceration. Eventually, 47 % of the admitted patients are treated by skin transplantation.Conclusions: This questionnaire gives a good insight in the epidemiology, and the diagnostic and therapeutic regimen for patients with venous leg ulcers in Dutch dermatologic practice.
Multilinear generalized Radon transforms and point configurations
Authors: Grafakos Loukas , Greenleaf Allan , Iosevich Alex , Palsson Eyvindur 
Published: September 2013

Abstract
We study multilinear generalized Radon transforms using a graph-theoretic paradigm that includes the widely studied linear case.
These provide a general mechanism to study Falconer-type problems involving (k+1)-point configurations in geometric measure theory, with k ≥ 2, including the distribution of simplices, volumes and angles determined by the points of
fractal subsets E ⊂ ℝ
d
, d ≥ 2.
If Tk
(E) denotes the set of noncongruent

(
k
+
1
)

${(k+1)}$

-point configurations determined by E, we show that if the Hausdorff dimension of E is greater than d - (d-1)/(2k), then the

k
+
1

2

${\binom{k+1}{2}}$

-dimensional Lebesgue measure of Tk
(E) is positive. This complements previous work on the Falconer conjecture ([Int. Math. Res. Not. IMRN 23 (2005), 1411–1425] and the references there), as well as work on finite point configurations [Recent Advances in Harmonic Analysis and Applications, Springer-Verlag, New York (2013), 93–103; Anal. PDE
5 (2012), no. 2, 397–409]. We also give applications to
Erdős-type problems in discrete geometry and a fractal regular value theorem, providing a multilinear framework for the results in [Adv. Math. 228 (2011), 2385–2402].
High impact toughness of polyamide 6/poly (vinylidene fluoride) blends induced by an ionic liquid
Authors: Li Chun , Na Bing , Lv Ruihua , Zhu Jie , Pan Huayan , Yu Zhiwei 
Published: September 2013

Abstract
This study reports the high impact toughness of polyamide 6 (PA6)/poly (vinylidene fluoride) (PVDF) blends achieved via adding an ionic liquid. The presence of the ionic liquid in the blends promotes the transformation of sea-island structure into co-continuous morphology, which to a large extent accounts for the improvement in toughness. In addition, it is believed that the enhanced mobility of the PA6 matrix by the ionic liquid also contributes to the high impact toughness.
INVESTIGATION ON MECHANICAL PROPERTIES OF A356 NANOCOMPOSITES FABRICATED BY ULTRASONIC ASSISTED CAVITATION
Authors: Donthamsetty Suneel , D Nageswara Rao. 
Published: September 2013

This paper presents mechanical properties of A356 matrix nanocomposites fabricated by reinforcing nano silicon carbide (SiC) particles at 0.1 to 0.5 weight percent with the aid of ultrasonic cavitation. SiC nanoparticles synthesized by bottom-up and top-down approach are used in this work as reinforcements and change in mechanical properties are observed. For comparision, A356 matrix composite reinforced with microparticles at 0.5 wt% by stir casting also prepared. Till now, SiC nanoparticles produced by bottom up approach were used as reinforcements in A356 alloy by so many researchers to improve mechanical properties. But in this work, nanoparticles produced by high energy ball milling (Top-down approach) are also used as reinforcements. It is observed that there is reduction in tensile properties for the composites prepared using nanoparticles via top down approach than that of bottom up approach. But significant increase compared to the composites prepared by reinforcing same amount of microparticles by stir casting. The reasons may be due to the non-uniformity in size of particles and contamination while milling nanoparticles. However, the change in properties is less and use of nanoparticles via top down approach is admissible when compared to the cost of nanoparticles synthesized from bottom up approach or chemical routes.DOI: http://dx.doi.org/10.3329/jme.v41i2.7507
Perfect Orderings on Finite Rank Bratteli Diagrams
Authors: Bezuglyi S. , Kwiatkowski J. , Yassawi R. 
Published: September 2013

AbstractGiven a Bratteli diagram B, we study the set 𝒪B of all possible orderings on B and its subset PB consisting of perfect orderings that produce Bratteli–Vershik topological dynamical systems (Vershik maps). We give necessary and sufficient conditions for the ordering ω to be perfect. On the other hand, a wide class of non-simple Bratteli diagrams that do not admit Vershik maps is explicitly described. In the case of finite rank Bratteli diagrams, we show that the existence of perfect orderings with a prescribed number of extreme paths constrains significantly the values of the entries of the incidence matrices and the structure of the diagram B. Our proofs are based on the new notions of skeletons and associated graphs, defined and studied in the paper. For a Bratteli diagram B of rank k, we endow the set 𝒪B with product measure μ and prove that there is some 1 ≤ j ≤ k such that μ-almost all orderings on B have j maximal and j minimal paths. If j is strictly greater than the number of minimal components that B has, then μ-almost all orderings are imperfect.