bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of enamel matrix derivative on the proliferation and osteogenic differentiation of human gingival mesenchymal stem cells
Authors: Wu Shu-Man , Chiu Hsien-Chung , Chin Yu-Tang , Lin Heng-Yi , Chiang Cheng-Yang , Tu Hsiao-Pei , Fu Martin MJ , Fu Earl 
Published: April 2014

Abstract
Introduction
Gingiva-derived mesenchymal stem cells (GMSCs) have recently been harvested and applied for rebuilding lost periodontal tissue. Enamel matrix derivative (EMD) has been used for periodontal regeneration and the formation of new cementum with inserting collagen fibers; however, alveolar bone formation is minimal. Recently, EMD has been shown to enhance the proliferation and mineralization of human bone marrow mesenchymal stem cells. Because the gingival flap is the major component to cover the surgical wound, the effects of EMD on the proliferation and mineralization of GMSCs were evaluated in the present study.

Methods
After single cell suspension, the GMSCs were isolated from the connective tissues of human gingiva. The colony forming unit assay of the isolated GMSCs was measured. The expression of stem cell markers was examined by flow cytometry. The cellular telomerase activity was identified by polymerase chain reaction (PCR). The osteogenic, adipogenic and neural differentiations of the GMSCs were further examined. The cell proliferation was determined by MTS assay, while the expression of mRNA and protein for mineralization (including core binding factor alpha, cbfα-1; alkaline phosphatase, ALP; and osteocalcin, OC; ameloblastin, AMBN) were analyzed by real time-PCR, enzyme activity and confocal laser scanning microscopy.

Results
The cell colonies could be easily identified and the colony forming rates and the telomerase activities increased after passaging. The GMSCs expressed high levels of surface markers for CD73, CD90, and CD105, but showed low expression of STRO-1. Osteogenic, adipogenic and neural differentiations were successfully induced. The proliferation of GMSCs was increased after EMD treatment. ALP mRNA was significantly augmented by treating with EMD for 3 hours, whereas AMBN mRNA was significantly increased at 6 hours after EMD treatment. The gene expression of OC was enhanced at the dose of 100 μg/ml EMD at day 3. Increased protein expression for cbfα-1 at day 3, for ALP at day 5 and 7, and for OC at week 4 after the EMD treatments were observed.

Conclusions
Human GMSCs could be successfully isolated and identified. EMD treatments not only induced the proliferation of GMSCs but also enhanced their osteogenic differentiation after induction.
Reduced consumption of protein-rich foods follows immune challenge in a polyphagous caterpillar
Authors: Mason Peri A. , Smilanich Angela M. , Singer Michael S. 
Published: April 2014

AbstractAdvances in ecological immunity have illustrated that, like vertebrates, insects exhibit adaptive immunity, including induced changes in feeding behavior that aid the immune system. In particular, recent studies have pointed to the importance of protein intake in mounting an immune response. In this study, we tested the hypothesis that the polyphagous caterpillar, Grammia incorrupta (Hy. Edwards, Erebidae), would adaptively change its feeding behavior in response to immune challenge, predicting that caterpillars would increase their intake of dietary protein. We further predicted that this response would enhance the melanization response, a component of the immune system that acts against parasitoids. We challenged the immune system using either tachinid fly parasitoids or a bead injection technique that has been used in studies to simulate parasitism, and measured feeding before and after immune challenge on diets varying in their macronutrient content. To evaluate the effects of diet on melanization, we quantified melanization of beads following feeding assays. Contrary to our prediction, we found that parasitized or injected caterpillars given a choice between high and low protein foods reduced their intake of the high protein food. Furthermore, in a no-choice experiment, caterpillars offered food with a protein concentration that is optimal for growth reduced feeding following immune challenge, whereas those offered a low protein food did not. Although variation in protein intake did not change caterpillars' melanization response, increased carbohydrate intake did increase melanization, suggesting a prophylactic role for carbohydrates. We discuss alternative mechanisms by which variation in protein intake could negatively or positively affect parasitized caterpillars, including nutritional interactions with the caterpillar's self-medication response.
Energy metabolism and regeneration impaired by seawater acidification in the infaunal brittlestar,Amphiura filiformis
Authors: Hu Marian Y. , Casties Isabel , Stumpp Meike , Ortega-Martinez Olga , Dupont Sam T. 
Published: April 2014

AbstractSeawater acidification due to anthropogenic release of CO2 as well as the potential leakage of pure CO2 from sub-seabed carbon capture storage sites (CCS) may impose a serious threat to marine organisms. Although infaunal organisms can be expected to be particularly impacted by decreases in seawater pH, due to naturally acidified conditions in benthic habitats, information regarding physiological and behavioral responses is still scarce. Determination of pO2 and pCO2 gradients within the burrows of the brittlestar Amphiura filiformis during environmental hypercapnia demonstrated that besides hypoxic conditions, increases of environmental pCO2 are additive to the already high pCO2 (up to 0.08 kPa) within the burrows. In response to up to 4 weeks exposure to pH 7.3 (0.3 kPa pCO2) and pH 7.0 (0.6 kPa pCO2), metabolic rates of A.filiformis were significantly reduced in pH 7.0 treatments accompanied by increased ammonium excretion rates. Gene expression analyses demonstrated significant reductions of acid-base (NBCe and AQP9) and metabolic (G6PDH, LDH) genes. Determination of extracellular acid-base status indicated an uncompensated acidosis in CO2 treated animals, which could explain depressed metabolic rates. Metabolic depression is associated with a retraction of filter feeding arms into sediment burrows. Regeneration of lost arm tissues following traumatic amputation is associated with significant increases in metabolic rate, and hypercapnic conditions (pH 7.0, 0.6 KPa) dramatically reduce the metabolic scope for regeneration reflected in 80% reductions in regeneration rate. Thus, the present work demonstrates that elevated seawater pCO2 significantly affects the environment and the physiology of infaunal organisms like A. filiformis.
Diurnal dung beetles use the intensity gradient and the polarization pattern of the sky for orientation
Authors: el Jundi Basil , Smolka Jochen , Baird Emily , Byrne Marcus J. , Dacke Marie 
Published: April 2014

Abstract
To escape competition at the dung pile, a ball-rolling dung beetle forms a piece of dung into a ball and rolls it away. To ensure an their efficient escape from the dung pile, the beetles rely on a celestial compass to move along a straight paths. Here, we analyzed the reliability of different skylight cues for this compass and found that dung beetles rely not only on the sun, but also on the skylight polarization pattern. Moreover, we show the first evidence of an insect using the celestial light intensity gradient for orientation. Using a polarizer, we manipulated skylight so that the polarization pattern appeared to turn by 90°. The beetles then changed their bearing close to the expected 90°. This behavior was abolished if the sun was visible to the beetle, suggesting that polarized light is hierarchically subordinate to the sun. If the sky was depolarized and the sun was invisible, the beetles could still move along straight paths. We therefore analyzed the use of the celestial intensity gradient for orientation. Artificially rotating the intensity pattern by 180° caused beetles to orient in the opposite direction. The intensity cue was also found to be subordinate to the sun, and could play a role in disambiguating the polarization signal, especially at low sun elevations.
Defensive slime formation in Pacific hagfish requires Ca2+ and aquaporin mediated swelling of released mucin vesicles
Authors: Herr Julia E. , Clifford Alex , Goss Greg G. , Fudge Douglas S. 
Published: April 2014

AbstractHagfishes defend themselves from fish predators via the rapid deployment of a fibrous slime that adheres to and clogs gills. The slime transforms from a thick glandular exudate to a fully hydrated product in a fraction of a second through a process that involves the swelling and rupture of numerous mucin vesicles. Here we demonstrate that the vesicle membrane plays an important role in regulating the swelling of mucin granules, and provide evidence that the membrane contains proteins that facilitate the movement of ions and water molecules. By exposing isolated mucin vesicles to varying combinations of inorganic ions, organic compounds, and membrane channel inhibitors, we found that the majority of hagfish mucin vesicles require Ca2+ to rupture. We also show that Ca2+-dependent rupture can be pharmacologically inhibited, which suggests a role for Ca2+-activated membrane transporters. We demonstrate that the aquaporin inhibitor mercuric chloride reduces the rate of vesicle swelling by an order of magnitude, which suggests that aquaporins facilitate the influx of water during vesicle deployment. Molecular evidence of two aquaporin homologs expressed in the slime glands further supports this idea. We propose a model of hagfish slime mucin vesicle rupture that involves Ca2+-activated transporters and aquaporins, and suggest that the presence of these proteins is an adaptation for increasing the speed of vesicle rupture and consequently the speed of the sliming response of hagfishes.
Rapid swimming and escape movements in the aquatic larvae and pupae of the phantom midgeChaoborus(Diptera, Chaoboridae)
Authors: Burrows Malcolm , Dorosenko Marina 
Published: April 2014

AbstractRapid locomotion in the aquatic larvae and pupae of the phantom midge Chaoborus was analysed. A 10 mm long larva moved sporadically by rapidly curling into a tight circle and then unfurling. The most common movement (70% of all movements) was a body rotation of 332 ± 22 degrees that lasted 63 ± 19 ms and reached a peak velocity of 0.07 ± 0.02 m s-1. If the head unfurled earlier in the cycle, the rotation was smaller and the larva dived downwards. A distinct category of single rotations of about 180 degrees (8%) resulted in a larva finishing with its head pointing in the opposite direction. A sequence of rotational movements (22%) resulted in more extensive displacements. The area of the tail fan was reduced by folding during part of a cycle. It was made of a row of 26 radiating filaments with interlacing hairs between adjacent filaments and resilin at their ventral midline articulations with the body. The fan sprang back passively to its splayed position after being forcibly folded. Reducing the area of the fan by 80% decreased angular rotation and impaired stability so that 33% of movements ended with the body upside down. A 6 mm long pupa also moved by curling and unfurling motions of the head and tail that lasted 215 ± 19 ms and generated slower velocities of 0.03 ± 0.01 m s-1. The pupal tail fan was membranous, oriented differently, had resilin at its articulations and its area could be changed.
Design and Fabrication of a Silicone Rubber-based Mediatorless Bioelectrode for Oxygen Reduction
Authors: Chen Meng Ying , Chen Xiao Dong , Wu Xue E 
Published: April 2014

Abstract
In this work, a mediatorless bioelectrode has been constructed for the first time on a flexible silicone rubber sheet coated with homogeneous chitosan–carbon nanotubes composites. Bilirubin oxidase was employed as a model biocatalyst to reduce O2 to H2O. These novel fabricated electrodes showed good electrochemical properties and exhibited comparatively high operational stability even after 5 days of continuous operation. The results obtained here demonstrated the potential of silicone rubber-based materials as a promising platform for flexible bioelectronics in medical devices.
Effect of small amount of alumina on structure, wear and mechanical properties of 3Y-TZP ceramics
Authors: Danilenko Igor , Prokhorenko Serhii , Konstantinova Tetyana , Ahkozov Leonid , Burkhovetski Valerii , Glazunova Valentina 
Published: April 2014

The use of ceramic instead of metallic parts in devices that operate in aggressive conditions increases the service life of machines and equipment for chemical, metallurgical and other industries. The wear resistant zirconia/alumina composites were sintered from nanopowders obtained by co-precipitation technique. In the case of addition of 1wt% of alumina in zirconia ceramics the wear resistance increased by approximately 30%.The formation of complex multilevel composite structures, such as Al3+ ion segregation on zirconia grain boundaries and intracrystalline alumina inclusions in zirconia grains, increased the fracture toughness values of composites obtained from co-precipitated nanopowders and consequently decreased the volume loss of ceramic material.In this study, we investigated the effect of nanopowders synthesis methods and alumina concentration on composite structure, fracture toughness and tribological behavior of 3Y-TZP/alumina ceramic composites and searched correlation between structures and mechanical properties.
Emergent Life Cycle: The Tension Between Knowledge Change and Knowledge Retention in Open Online Coproduction Communities
Authors: Kane Gerald C. , Johnson Jeremiah , Majchrzak Ann 
Published: April 2014

Online coproduction communities often face a challenge of whether to change or retain the knowledge they have created. Disparate and often conflicting theoretical models have been used to explain how these communities respond to this tension. We conducted a case study of how one online coproduction community—the nine-year history of the Wikipedia article on autism—handles this tension. We find that the nature of the change–retain tension and the community’s response to it fluctuates considerably over the life of the community. These changes bear striking similarities to processes associated with traditional software development life cycles, despite the absence of traditional control mechanisms. What initially appear to be conflicts in the extant literature actually describe different roles and production focus at the different stages of development. Disruptive events signal the need for the community to shift production focus, which often involves members joining and leaving the production process, rather than adopting new roles.This paper was accepted by Sandra Slaughter, information systems.
Integration of Online and Offline Channels in Retail: The Impact of Sharing Reliable Inventory Availability Information
Authors: Gallino Santiago , Moreno Antonio 
Published: April 2014

Using a proprietary data set, we analyze the impact of the implementation of a “buy-online, pick-up-in-store” (BOPS) project. The implementation of this project is associated with a reduction in online sales and an increase in store sales and traffic. These results can be explained by two simultaneous phenomena: (1) additional store sales from customers who use the BOPS functionality and buy additional products in the stores (cross-selling effect) and (2) the shift of some customers from the online to the brick-and-mortar channel and the conversion of noncustomers into store customers (channel-shift effect). We explain these channel-shift patterns as an increase in “research online, purchase offline” behavior enabled by BOPS implementation, and we validate this explanation with evidence from the change of cart abandonment and conversion rates of the brick-and-mortar and online channels. We interpret these results in light of recent operations management literature that analyzes the impact of sharing inventory availability information. Our analysis illustrates the limitations of drawing conclusions about complex interventions using single-channel data. This paper was accepted by Alok Gupta, special issue on business analytics.
Path to Purchase: A Mutually Exciting Point Process Model for Online Advertising and Conversion
Authors: Xu Lizhen , Duan Jason A. , Whinston Andrew 
Published: April 2014

This paper studies the effects of various types of online advertisements on purchase conversion by capturing the dynamic interactions among advertisement clicks themselves. It is motivated by the observation that certain advertisement clicks may not result in immediate purchases, but they stimulate subsequent clicks on other advertisements, which then lead to purchases. We develop a novel model based on mutually exciting point processes, which consider advertisement clicks and purchases as dependent random events in continuous time. We incorporate individual random effects to account for consumer heterogeneity and cast the model in the Bayesian hierarchical framework. We construct conversion probability to properly evaluate the conversion effects of online advertisements. We develop simulation algorithms for mutually exciting point processes to compute the conversion probability and for out-of-sample prediction. Model comparison results show the proposed model outperforms the benchmark models that ignore exciting effects among advertisement clicks. Using a proprietary data set, we find that display advertisements have relatively low direct effect on purchase conversion, but they are more likely to stimulate subsequent visits through other advertisement formats. We show that the commonly used measure of conversion rate is biased in favor of search advertisements and underestimates the conversion effect of display advertisements the most. Our model also furnishes a useful tool to predict future purchases and advertisement clicks for the purpose of targeted marketing and customer relationship management.This paper was accepted by Eric Bradlow, special issue on business analytics.
Hazardous Drug Residue on Exterior Vial Surfaces: Evaluation of a Commercial Manufacturing Process
Authors: Power Luci A. , Sessink Paul J. M. , Gesy Kathy , Charbonneau Flay 
Published: April 2014

Purpose Hazardous drug residue on the exterior surface of drug vials poses a potential risk for exposure of health care workers involved in handling these products. The purpose of this article is to heighten the awareness of this serious issue and to evaluate a commercial manufacturing process for removing and containing hazardous drug (HD) residue on exterior vial surfaces. Additionally, findings from this study are interpreted, incorporated into the current body of evidence, and discussed by experts in this field. Methods This study includes separate evaluations for the presence or absence of surface drug contamination on the vials of 3 HD products: 5-fluorouracil, cisplatin, and methotrexate. The drug products were packaged in vials using a patented prewashing/decontamination method, application of a polyvinylchloride (PVC) base, and use of clear glass vials. An additional step of encasing the vial in a shrink-wrapped sheath was used for 5-fluorouracil and cisplatin. Results Of all 5-fluorouracil (110 vials), methotrexate (60 vials), and cisplatin (60 vials) tested, only 2 had detectable amounts of surface residue. One 5-fluorouracil vial was found to have approximately 4 mg of 5-fluorouracil on the surface of the vial. The second contaminated vial was cisplatin, which was discovered to have 131 ng of platinum, equal to 200 ng of cisplatin or 0.2 μL of cisplatin solution, on the vial sheath. Conclusion Using validated extraction and analytic methods, all but 2 of the 230 tested vials were found to be free of surface drug contamination. Pharmacy leaders need to take an active role in promoting the need for clean HD vials. Manufacturers should be required to provide their clients with data derived from externally validated analytic studies, reporting the level of HD contamination on the exterior of their vial products.
Edc3 Function in Yeast and Mammals Is Modulated by Interaction with NAD-Related Compounds
Authors: Walters Robert W , Shumilin Igor A , Yoon Je-Hyun , Minor Wladek , Parker Roy 
Published: April 2014

Abstract
The control of mRNA translation and degradation is mediated in part by a set of proteins that can inhibit translation and promote decapping, as well as function in the assembly of cytoplasmic mRNP granules referred to as processing bodies (P-bodies). The conserved enhancer of mRNA decapping 3 (Edc3) protein functions to promote both decapping and P-body assembly. Crystal structures of the YjeF_N domain in hEdc3 identified a putative binding site for a small molecule. Structure modeling of the human Edc3 Yjef_N along with other Yjef_N-containing proteins suggests that this molecule is related to NAD(H). We now show human Edc3 directly binds NADH. We also show that human and yeast Edc3 chemically modify NAD in vitro. Mutations that are predicted to disrupt the binding and/or hydrolysis of an NAD-related molecule by yeast and human Edc3 affect the control of mRNA degradation and/or P-body composition in vivo. This suggests that the interaction of Edc3 with an NAD-related molecule affects its function in the regulation of mRNA translation and degradation and provides a possible mechanism to couple the energetics of the cell to posttranscriptional control. Moreover, this provides a unique example of and lends strength to the postulated connection of metabolites, enzymes, and RNA.
DAIRRy-BLUP: A High-Performance Computing Approach to Genomic Prediction
Authors: Coninck Arne De , Fostier Jan , Maenhout Steven , De Baets Bernard 
Published: April 2014

Abstract
In genomic prediction, common analysis methods rely on a linear mixed-model framework to estimate SNP marker effects and breeding values of animals or plants. Ridge regression–best linear unbiased prediction (RR-BLUP) is based on the assumptions that SNP marker effects are normally distributed, are uncorrelated, and have equal variances. We propose DAIRRy-BLUP, a parallel, Distributed-memory RR-BLUP implementation, based on single-trait observations (y), that uses the Average Information algorithm for restricted maximum-likelihood estimation of the variance components. The goal of DAIRRy-BLUP is to enable the analysis of large-scale data sets to provide more accurate estimates of marker effects and breeding values. A distributed-memory framework is required since the dimensionality of the problem, determined by the number of SNP markers, can become too large to be analyzed by a single computing node. Initial results show that DAIRRy-BLUP enables the analysis of very large-scale data sets (up to 1,000,000 individuals and 360,000 SNPs) and indicate that increasing the number of phenotypic and genotypic records has a more significant effect on the prediction accuracy than increasing the density of SNP arrays.