bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the model‐based optimization of secreting mammalian cell (GS‐NS0) cultures
Authors: Kiparissides A. , Pistikopoulos E. N. , Mantalaris A. 
Published: September 2014

ABSTRACTThe global bio‐manufacturing industry requires improved process efficiency to satisfy the increasing demands for biochemicals, biofuels, and biologics. The use of model‐based techniques can facilitate the reduction of unnecessary experimentation and reduce labor and operating costs by identifying the most informative experiments and providing strategies to optimize the bioprocess at hand. Herein, we investigate the potential of a research methodology that combines model development, parameter estimation, global sensitivity analysis, and selection of optimal feeding policies via dynamic optimization methods to improve the efficiency of an industrially relevant bioprocess. Data from a set of batch experiments was used to estimate values for the parameters of an unstructured model describing monoclonal antibody (mAb) production in GS‐NS0 cell cultures. Global Sensitivity Analysis (GSA) highlighted parameters with a strong effect on the model output and data from a fed‐batch experiment were used to refine their estimated values. Model‐based optimization was used to identify a feeding regime that maximized final mAb titer. An independent fed‐batch experiment was conducted to validate both the results of the optimization and the predictive capabilities of the developed model. The successful integration of wet‐lab experimentation and mathematical model development, analysis, and optimization represents a unique, novel, and interdisciplinary approach that addresses the complicated research and industrial problem of model‐based optimization of cell based processes. Biotechnol. Bioeng. 2015;112: 536–548. © 2014 Wiley Periodicals, Inc.
The persuasive effectiveness of mini‐films: Narrative transportation and fantasy proneness
Authors: Chen Tsai 
Published: September 2014

AbstractLittle empirical investigation has been made on mini‐films, an innovative online marketing technique of branded entertainment. This study intends to gain a deeper understanding of the advertising effectiveness of mini‐films. Employing a narrative persuasion research approach, an online survey with hyperlinks to a variety of real mini‐films was conducted to test the aggregate persuasive effectiveness of this marketing innovation. The results demonstrated that narrative transportation has a major influence on the attitude toward the mini‐film; the mini‐film attitude fully mediates the effect of narrative transportation to brand attitude, and subsequently to purchase intention. Furthermore, conceptualized as a normal consumer characteristic, the influence of fantasy proneness on the persuasive mechanism of this online advertising genre has also been under study. Empirical evidence showed that fantasy proneness has a positive influence on narrative transportation. This study has made a contribution to academics and practitioners alike for future research into branded entertainment. Copyright © 2014 John Wiley & Sons, Ltd.
Catalysis via Sol–Gel Acid Silicas: An Important Chemical Technology for 2nd Generation Biorefineries
Authors: Ciriminna Rosaria , Demma Carà Piera , Lopez‐Sanchez Jose A. , Pagliaro Mario 
Published: September 2014

AbstractHeterogeneously catalyzed processes using mesoporous acid silicas selectively afford many valued products from biomass components. The versatile nanochemistry of the sol–gel process allows to shape materials with catalytically attractive morphologies, as well as to further functionalize the inner mesoporosity with other active species affording nanocomposites suitable for consecutive reactions in one‐pot. Using selected examples from recent chemical research, we identify an approach that will likely produce catalysts of enhanced performance, suitable for practical application.
Homogeneous Photocatalytic Reduction of CO2 to CO Using Iron(0) Porphyrin Catalysts: Mechanism and Intrinsic Limitations
Authors: Bonin Julien , Chaussemier Marie , Robert Marc , Routier Mathilde 
Published: September 2014

AbstractA photochemical catalytic reduction of CO2 was performed in an organic solvent with iron(0) porphyrins as homogeneous molecular catalysts under visible light irradiation. With modified tetraphenylporphyrins consisting of internal phenolic groups, the photochemical process led to the production of CO, with H2 as a minor product. High catalytic selectivity for CO formation and turnover numbers up to 30 were obtained. Degradation of the catalyst occurred at longer irradiation times, along with decreased selectivity. Furthermore, addition of a weak acid, which increased the reduction efficiency under electrochemical conditions, led to rapid deactivation of the catalyst. With the unmodified tetraphenylporphyrin as catalyst, we observed lower performance and higher proportion of H2, which highlighted differences in the reduction pathways followed. A combination of a spectroscopic study and product analysis performed under various conditions led to detailed reduction mechanisms and helped pave the way for designing durable photocatalytic systems.
Combined Experimental and Theoretical Study on the Reactivity of Compounds I and II in Horseradish Peroxidase Biomimetics
Authors: Ji Li , Franke Alicja , Brindell Małgorzata , Oszajca Maria , Zahl Achim , van Eldik Rudi 
Published: September 2014

AbstractFor the exploration of the intrinsic reactivity of two key active species in the catalytic cycle of horseradish peroxidase (HRP), Compound I (HRP‐I) and Compound II (HRP‐II), we generated in situ [FeIVO(TMP+.)(2‐MeIm)]+ and [FeIVO(TMP)(2‐MeIm)]0 (TMP=5,10,15,20‐tetramesitylporphyrin; 2‐MeIm=2‐methylimidazole) as biomimetics for HRP‐I and HRP‐II, respectively. Their catalytic activities in epoxidation, hydrogen abstraction, and heteroatom oxidation reactions were studied in acetonitrile at −15 °C by utilizing rapid‐scan UV/Vis spectroscopy. Comparison of the second‐order rate constants measured for the direct reactions of the HRP‐I and HRP‐II mimics with the selected substrates clearly confirmed the outstanding oxidizing capability of the HRP‐I mimic, which is significantly higher than that of HRP‐II. The experimental study was supported by computational modeling (DFT calculations) of the oxidation mechanism of the selected substrates with the involvement of quartet and doublet HRP‐I mimics (2,4Cpd I) and the closed‐shell triplet spin HRP‐II model (3Cpd II) as oxidizing species. The significantly lower activation barriers calculated for the oxidation systems involving 2,4Cpd I than those found for 3Cpd II are in line with the much higher oxidizing efficiency of the HRP‐I mimic proven in the experimental part of the study. In addition, the DFT calculations show that all three reaction types catalyzed by HRP‐I occur on the doublet spin surface in an effectively concerted manner, whereas these reactions may proceed in a stepwise mechanism with the HRP‐II mimic as oxidant. However, the high desaturation or oxygen rebound barriers during CH bond activation processes by the HRP‐II mimic predict a sufficient lifetime for the substrate radical formed through hydrogen abstraction. Thus, the theoretical calculations suggest that the dissociation of the substrate radical may be a more favorable pathway than desaturation or oxygen rebound processes. Importantly, depending on the electronic nature of the oxidizing species, that is, 2,4Cpd I or 3Cpd II, an interesting region‐selective conversion phenomenon between sulfoxidation and H‐atom abstraction was revealed in the course of the oxidation reaction of dimethylsulfide. The combined experimental and theoretical study on the elucidation of the intrinsic reactivity patterns of the HRP‐I and HRP‐II mimics provides a valuable tool for evaluating the particular role of the HRP active species in biological systems.
Rational Design of Heat‐Set and Specific‐Ion‐Responsive Supramolecular Hydrogels Based on the Hofmeister Effect
Authors: Nebot Vicent J. , Ojeda‐Flores Juan J. , Smets Johan , Fernández‐Prieto Susana , Escuder Beatriu , Miravet Juan F. 
Published: September 2014

AbstractSmart supramolecular hydrogels have been prepared from a bolaamphiphilic L‐valine derivative in aqueous solutions of different salts. The hydrogels respond selectively to different ions and are either reinforced or weakened. In one case, in contrast to conventional systems, the hydrogels are formed upon heating of the system. The use of the hydrogels in the controlled release of an entrapped dye is described as a proof of the potential applications of these systems. The responsive hydrogels were rationally designed by taking into account the noticeable effect of different ions from the Hofmeister series in the solubility of the hydrogelator, which was assessed by using NMR experiments. On the one hand, kosmotropic anions such as sulfate produce a remarkable solubility decrease in the gelator, which is associated with gel reinforcement, as measured by rheological experiments. On the other hand, chaotropic species such as perchlorate weaken the gel. A dramatic effect was observed in the presence of guanidinium chloride, which boosted the solubility of the gelator, in accordance with its chaotropic behaviour reported in protein science. In this case, a direct interaction of the guanidinium species with the carbonyl groups of the hydrogelator is detected by 13C NMR spectroscopy. The weakening of this interaction upon a temperature increase allows for the preparation of heat‐set hydrogelating systems.
Discovery of Methyl 4′‐Methyl‐5‐(7‐nitrobenzo[c][1,2,5]oxadiazol‐4‐yl)‐[1,1′‐biphenyl]‐3‐carboxylate, an Improved Small‐Molecule Inhibitor of c‐Myc–Max Dimerization
Authors: Chauhan Jay , Wang Huabo , Yap Jeremy L. , Sabato Philip E. , Hu Angela , Prochownik Edward V. , Fletcher Steven 
Published: September 2014

Abstractc‐Myc is a basic helix‐loop‐helix‐leucine zipper (bHLH‐ZIP) transcription factor that is responsible for the transcription of a wide range of target genes involved in many cancer‐related cellular processes. Over‐expression of c‐Myc has been observed in, and directly contributes to, a variety of human cancers including those of the hematopoietic system, lung, prostate and colon. To become transcriptionally active, c‐Myc must first dimerize with Myc‐associated factor X (Max) via its own bHLH‐ZIP domain. A proven strategy towards the inhibition of c‐Myc oncogenic activity is to interfere with the structural integrity of the c‐Myc–Max heterodimer. The small molecule 10074‐G5 is an inhibitor of c‐Myc–Max dimerization (IC50=146 μM) that operates by binding and stabilizing c‐Myc in its monomeric form. We have identified a congener of 10074‐G5, termed 3jc48‐3 (methyl 4′‐methyl‐5‐(7‐nitrobenzo[c][1,2,5]oxadiazol‐4‐yl)‐[1,1′‐biphenyl]‐3‐carboxylate), that is about five times as potent (IC50=34 μM) at inhibiting c‐Myc–Max dimerization as the parent compound. 3jc48‐3 exhibited an approximate twofold selectivity for c‐Myc–Max heterodimers over Max–Max homodimers, suggesting that its mode of action is through binding c‐Myc. 3jc48‐3 inhibited the proliferation of c‐Myc‐over‐expressing HL60 and Daudi cells with single‐digit micromolar IC50 values by causing growth arrest at the G0/G1 phase. Co‐immunoprecipitation studies indicated that 3jc48‐3 inhibits c‐Myc–Max dimerization in cells, which was further substantiated by the specific silencing of a c‐Myc‐driven luciferase reporter gene. Finally, 3jc48‐3′s intracellular half‐life was >17 h. Collectively, these data demonstrate 3jc48‐3 to be one of the most potent, cellularly active and stable c‐Myc inhibitors reported to date.
Arylsulfonyl Groups: The Best Cyclization Auxiliaries for the Preparation of ATRC γ‐Lactams can be Acidolytically Removed
Authors: Clark Andrew J. , Cornia Andrea , Felluga Fulvia , Gennaro Armando , Ghelfi Franco , Isse Abdirisak A. , Menziani Maria Cristina , Muniz‐Miranda Francesco , Roncaglia Fabrizio , Spinelli Domenico 
Published: September 2014

AbstractThe N‐arylsulfonyl group, which is the best and most useful cyclization auxiliary for the transition‐metal‐catalyzed atom transfer radical cyclization (ATRC) of N‐allyl‐α‐polychloroamides, can be effectively removed from the target γ‐lactams by using H2SO4–HOAc, without impairing the halogen functions. The reaction involves H+ attack on the aromatic moiety, and is strongly responsive to the electronic properties of the substituent bound to the aromatic ring: electron‐donating groups, such as methyl or methoxy are, in fact, required for efficient “deprotection”. The N‐p‐nitrophenylsulfonyl cyclization auxiliary, in contrast to all the other sulfonyl groups tested, proved to be unsuitable for the ATRC, owing to a side reductive transposition that halts the redox cycle.
Use of grape pomaces to produce biomass of a Komagataella pastoris strain expressing a bovine chymosin activity
Authors: Kingston Diego , Novelli Guido F. , Cerrutti Patricia , Recupero Matias N. , Blasco Martin , Galvagno Miguel A. 
Published: September 2014

AbstractThe use of agroindustrial wastes not only decreases bioprocesses and disposal costs but also contributes to the upgrading of the residues. An active recombinant methanol‐inducible bovine chymosin has been expressed in our laboratory in the yeast Komagataella pastoris, and grape pomace extracts (GRE) were proposed as a convenient C‐energy source for the biomass production of the genetically engineered strain. Carbon and nitrogen sources, growth factors, and initial pH conditions were selected by classical methodology; thereafter, growth conditions optimization was performed using statistical designed experiments (DoEs). In the presence of (in g·L−1) 67.0 monosaccharides (glucose and fructose) from GRE, 5.0 (NH4)2SO4, and 10.0 sugar cane molasses (CMz), a yield of 20.0 g·L−1 cell dry weight (CDW) was obtained aerobically after 60 h incubation at 28°C and pH 4.0. Applying a fed‐batch strategy with methanol:sorbitol as the enzyme inducers, a chymosin production of 8.53 International Milk Clotting Units (IMCU) per mg protein was obtained in the supernatant. The results presented show that through a statistical design, a simple, cheap, and easy to prepare culture medium could be developed using two agroindustrial derivatives (GRE and CMz) to obtain a higher value added product.
Management of the compromised airway and role of tracheotomy in anaplastic thyroid carcinoma
Authors: Mani Navin , McNamara Katherine , Lowe Natalie , Loughran Sean , Yap Beng K. 
Published: September 2014

ABSTRACTBackgroundAnaplastic thyroid carcinoma (ATC) is an uncommon thyroid malignancy with a poor prognosis. American Thyroid Association (ATA) guidelines acknowledge the complexity of airway management in these patients. We studied our local experience with the aim of providing guidance in airway management in ATC.MethodsPatients with histologically confirmed ATC from January 2004 to December 2011 were identified from our institutional database. The data were retrospectively analyzed using hospital case notes.ResultsTwenty‐six patients were identified with ATC, 25 of who died from the disease. Five of 26 patients (19%) had stridor at presentation. A further 6 of 26 patients (23%) developed stridor during or soon after radiotherapy. Nine patients (36%) died of airway obstruction.ConclusionTracheotomy can facilitate completion of palliative treatment in those patients with ATC and stridor. Given the short life expectancy of these patients, a balanced decision must be made regarding the role and timing of tracheotomy. © 2015 Wiley Periodicals, Inc. Head Neck 38: 85–88, 2016
Bi‐channel Surface Acoustic Wave Gas Sensor for Carbon Disulfide and Methanol Vapors in Polymer Plants
Authors: Chou Yu‐Chen , Shih Jeng‐Shong 
Published: September 2014

AbstractA C60‐polyphenylacetylene (C60‐PPA) and polyvinylpyrrolidone (PVP) coated two‐channel surface acoustic wave (SAW) crystal gas sensor with a homemade computer interface for data acquisition and data processing was developed and employed to detect carbon disulfide (CS2) and methanol (CH3OH) vapors in polymer plants. The frequency of surface acoustic wave oscillator decreases due to the adsorption of gas molecules on the coated materials of the SAW sensor. Six coating materials (C60‐PPA, nafion, PPA, crytand [2,2], polyethene glycol and PVP) were used to adsorb and detect carbon disulfide and methanol gases. Adsorption of all the six coating materials to CS2 and CH3OH was found to be physical adsorption. The C60‐PPA coated SAW detector exhibited more sensitive to CS2 than the other coating materials. In contrast, the PVP coated SAW detector was more sensitive to CH3OH than the other coating materials. With the two‐channel SAW sensor, the C60‐PPA coated SAW showed a good detection limit of 0.4 ppm and good reproducibility with RSD of 3.37 % (n=10) for CS2. Similarly, the PVP coated SAW also showed a good detection limit of 0.05 ppm and good reproducibility, with RSD of 0.86 % (n=10) for CH3OH. The interference effect of other organic molecules on the SAW detection system was negligible, except for the irreversible adsorption of C60‐PPA to propylamine. The frequency signals from the two‐channel SAW sensor array C60‐PPA and PVP coatings were processed by a back‐propagation artificial neural network (BPN) and multiple regression analysis (MRA). Thus a two‐channel SAW sensor array with BPN and MRA has been successfully applied for the qualitative and quantitative analyses of CS2 and CH3OH in mixtures.
ZnO: An Ecofriendly, Green Nano‐catalyst for the Synthesis of Pyrazole Derivatives under Aqueous Media
Authors: Girish Yarabally R. , Kumar Kothanahally S. Sharath , Manasa Heggadihalli S. , Shashikanth Sheena 
Published: September 2014

AbstractAn efficient nano ZnO catalyzed green protocol for the synthesis of pyrazol derivatives by condensation of different substituted phenyl hydrazines/semicarbazide/thiosemicarbazide with 1,3‐diketone/ketoester at ambient temperature has been achieved. ZnO nanocatalyst was prepared by low temperature solution combustion method. From the Scherrer method the crystallite size of ZnO was estimated and found to be in the range of 30–50 nm. The main advantage of this protocol is an excellent yield, short reaction time and easy work up procedure. The catalyst was found to be reusable up to five catalytic cycles without any appreciable loss in activity of the catalyst.
Liver transplantation for intrahepatic cholangiocarcinoma
Authors: Hashimoto Koji , Miller Charles M. 
Published: September 2014

AbstractThe indication of liver transplantation for intrahepatic cholangiocarcinoma (ICC) is highly controversial. Initially, liver transplantation was embraced as a promising treatment for ICC, providing both a wider surgical margin and a potential cure for the underlying liver disease. However, the majority of transplant centers have abandoned liver transplantation for ICC due to poor long‐term survival and high recurrence rates. Interestingly, these decisions were based on studies with highly inconsistent outcomes due to a limited number of patients, various patient selection criteria, and the use of nonstandardized adjunctive therapy protocols. Indeed, recent studies have revealed that ICC patients with small solitary tumors have excellent long‐term survival after liver transplantation. Moreover, as seen in early‐stage hilar cholangiocarcinoma, neoadjuvant and adjuvant therapy hold promise for improved long‐term survival in patients with locally advanced ICC. As we work to expand treatment options for ICC, further evidence of success in this area is needed in order to justify the use of limited organ resources to treat ICC. Continued efforts to improve diagnosis of ICC, hone patient selection criteria, and implement standardized treatment protocols could provide certain patients with ICC access to potentially life‐saving liver transplantation.
Effect of rosemary (Rosmarinus officinalis) extracts on the oxidative stability and sensory acceptability of soybean oil
Authors: Dias Liara S , Menis Michele E C , Jorge Neuza 
Published: September 2014

AbstractBACKGROUNDPlant extracts have been used as an alternative to the use of synthetic antioxidants in order to preserve oils from oxidative degradation. Additionally, these extracts add special flavors and aromas to the food. Thus, the objective of this study was to evaluate the effect of hydroethanolic extracts of fresh and freeze‐dried rosemary in the oxidative stability of soybean oil under accelerated storage in an oven.RESULTSThe application of the extracts in the oil showed that that freeze‐dried extract was better in reducing the formation of oxidation products, showing 8.6 meq kg−1 of peroxides after 20 days of storage. On the other hand, the mixture of the natural extract with t‐butylhydroquinone conferred better oxidative stability index until the 20th day, 9.7 h. Both extracts prevented the loss of tocopherol, not differing between each other (P > 0.05), and present approximately 505 mg kg−1 of residual tocopherols. The sensory evaluation revealed that consumers accepted equally the oils added and not added of the rosemary extracts.CONCLUSIONThe extracts are therefore potential sources of natural antioxidants and they would be well accepted by consumers if applied by the food industry to replace synthetic antioxidants. © 2014 Society of Chemical Industry
Transoral palate‐sparing nasopharyngectomy with the Flex®System: Preclinical study
Authors: Richmon Jeremy D. 
Published: September 2014

Objectives/HypothesisThe nasopharynx remains one of the most challenging areas to surgically access given its deep location, narrow confines, and surrounding critical neurovascular anatomy. Various open and endoscopic approaches have been developed, all of which have been limited to some degree in their ability to bimanually instrument and visualize in this area.Study DesignCadaveric dissection.MethodsStudy using one fresh human cadaver. A novel, highly articulated endoscope with flexible instrumentation, the Flex System, was placed in the oral cavity and advanced around the soft palate into the nasopharynx to perform a nasopharyngectomy.ResultsA complete nasopharyngectomy was accomplished with the Flex System without palatal incisions or extended‐access approaches.ConclusionsThe Flex System is a novel computer‐assisted flexible endoscope with a highly articulated scope and flexible instruments that was successfully used to perform a nasopharyngectomy while avoiding any additional incisions. This system holds promise in minimally invasive skull base surgery.Level of EvidenceNA Laryngoscope, 125:318–322, 2015