bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hand eczema among healthcare professionals in the Netherlands: prevalence, absenteeism, and presenteeism
Authors: van der Meer Esther W. C. , Boot Cécile R. L. , van der Gulden Joost W. J. , Jungbauer Frank H. W. , Coenraads Pieter Jan , Anema Johannes R. 
Published: June 2013

AbstractBackgroundHealthcare professionals have a high risk of developing hand eczema. Hand eczema can interfere with their work.ObjectivesTo investigate the prevalence of self‐reported hand eczema among healthcare professionals in the Netherlands, and to investigate absenteeism and presenteeism resulting from hand eczema.MethodsA questionnaire‐based observational study was performed. Participants were recruited from hospitals and nursing homes in the Netherlands. The study population consisted of 1232 healthcare professionals. We used the NOSQ‐2002 to measure hand eczema and the PRODISQ for absenteeism and presenteeism.ResultsThe 1‐year prevalence of hand eczema among healthcare professionals was 12%. Among all participants, 47% reported symptoms related to hand eczema. Sick leave resulting from hand eczema was reported by 0.3% of healthcare professionals in general, and by 1.7% of healthcare professionals with hand eczema. In the group with hand eczema, 3.1% reported a large effect on presenteeism.ConclusionsThe 1‐year prevalence of hand eczema among healthcare professionals in the Netherlands is low, but the prevalence of symptoms related to hand eczema is quite high. Hand eczema seems to have little impact on work in terms of absenteeism and presenteeism.
Effective models for correlating spectral reflectance between spectrophotometers
Authors: Xu Yang , Luo M Ronnier , Sun Pei‐Li , Li Changjun 
Published: June 2013

This paper describes different models to achieve satisfactory interinstrument agreement between spectrophometers. The models investigated include the Berns and Petersen method, the modified Berns and Petersen method, the Reniff method, the Rich and Martin method and the R‐model developed by Chung et al. In the paper, the Reniff method and the R‐model were extended, and all the models were then evaluated using sample sets with different substrates. It was found that the extended Reniff method gave the best training performance and the worst testing performance. The Rich and Martin model, the three‐term Chung model and the Berns and Petersen models, all with wavelength‐dependent coefficients, gave better testing performance.
Executive function in children and adolescents with unilateral cerebral palsy
Authors: Bodimeade Harriet L , Whittingham Koa , Lloyd Owen , Boyd Roslyn N 
Published: June 2013

AimThe aim of this study was to compare executive function in children with left‐ and right‐sided unilateral cerebral palsy (CP) and typically developing children.MethodThere was a cross‐sectional cohort of 46 children with unilateralCP(24 right‐side, 22 left‐side; 25 males, 21 females; mean age 11y 1mo,SD2y 5mo) and 20 typically developing children (nine males, 11 females; mean age 10y 10mo,SD2y 4mo). Four cognitive domains of executive function were assessed: attentional control, cognitive flexibility, goal setting, and information processing. Subtests from the Delis–Kaplan Executive Function System, the Test of Everyday Attention for Children, the Rey–Osterrieth Complex figure, and the Wechsler Intelligence Scale for Children – Fourth Edition were utilized. Between‐group differences (right unilateralCP, left unilateralCP, and typically developing children) were examined using analyses of covariance.ResultsChildren withCPperformed significantly more poorly than typically developing children on all executive function measures (aggregate executive function:F(1,63)=31.16;p<0.001; η2=0.33). There were no significant differences between children with left and right unilateralCP, except in the case of inhibition/switching total errors, with children with left unilateralCPmaking fewer errors than children with right unilateralCP(F(1,39)=4.14;p=0.049; η2=0.1).InterpretationChildren and adolescents with unilateralCPexperience difficulties across multiple executive function domains compared with typically developing children, irrespective of the side of hemiplegia. This finding supports an early vulnerability model of early brain injury and has implications for intervention for children withCP.
Cichlids respond to conspecific sounds but females exhibit no phonotaxis without the presence of live males
Authors: Estramil Natalia , Bouton Niels , Verzijden Machteld N. , Hofker Kees , Riebel Katharina , Slabbekoorn Hans 
Published: June 2013

AbstractMany fish species are able to produce sounds, which are often associated with courtship. In an earlier study, we showed for the Lake Victoria cichlid Pundamilia nyererei that females prefer to associate with a male with sound over a male without sound. As a follow‐up to this earlier finding, we here investigated whether playback of conspecific sounds is sufficient to attract females in the absence of a conspecific male. However, we did not find a phonotactic response for conspecific sounds in the absence of live males, using the same playback procedure as in our previous study. An additional playback test showed that both males and females discriminated between conspecific sounds and bursts of white noise. This suggests that the sounds may be recognised but that they seem only effective as attractant in the presence of visual and/or olfactory cues. These findings underline the multimodal complexity of fish communication and courtship and call for a more integrated study of the different modalities in future studies.
Elemental fingerprinting in otoliths reveals natal homing of anadromous Baltic Sea pike (Esox lucius L.)
Authors: Engstedt Olof , Engkvist Roland , Larsson Per 
Published: June 2013

AbstractWe examined the element pattern in the otoliths of a migratory fish species that inhabit the coastal areas in the brackish of the Baltic Sea. The northern pike (Esox lucius) show migratory behaviour, spawning in streams and rivers and foraging in the sea. We examined spawning migration in four nearby streams in the south‐west part of the Baltic. Otolith analysis by microPIXE revealed unique elemental patterns (Sr, Zn, Br, Co and Mn) for the juveniles in each of the different streams. The strontium signal in the otolith of the juveniles was used as an indicator of freshwater origin and the time spent in the stream. Adult pike in their migrating spawning phase were caught in each of the streams. The elemental composition in otoliths in their freshwater phase (using juvenile pike in the streams as references) was determined. A principal component analysis showed that the elemental fingerprint during the freshwater phase several years back in time was similar for the adult fish and for juveniles inhabiting the stream today. The results indicated natal homing of the adults to a specific stream, a conclusion that was strengthened by the fact that marked fish returned to spawn over consecutive years. Anadromous pike in the Baltic Sea may thus be divided in subpopulations. The results of the study may have implications for fishery management, as pike in the Baltic Sea cannot be seen as homogenous population.
Increased neocortical expression of the P2X7 receptor after status epilepticus and anticonvulsant effect of P2X7 receptor antagonist A‐438079
Authors: Jimenez‐Pacheco Alba , Mesuret Guillaume , Sanz‐Rodriguez Amaya , Tanaka Katsuhiro , Mooney Claire , Conroy Ronan , Miras‐Portugal Maria Teresa , Diaz‐Hernandez Miguel , Henshall David C. , Engel Tobias 
Published: June 2013

SummaryPurposeATP is an essential transmitter/cotransmitter in neuron function and pathophysiology and has recently emerged as a potential contributor to prolonged seizures (status epilepticus) through the activation of the purinergic ionotropic P2X7 receptor (P2X7R). Increased P2X7R expression has been reported in the hippocampus, and P2X7R antagonists reduced seizure‐induced damage to this brain region. However, status epilepticus also produces damage to the neocortex. The present study was designed to characterize P2X7R in the neocortex and assess effects of P2X7R antagonists on cortical injury after status epilepticus.MethodsStatus epilepticus was induced in mice by intraamygdala microinjection of kainic acid. Specific P2X7R inhibitors were administered into the ventricle before seizure induction, and cortical electroencephalography and behavior was recorded to assess seizure severity. P2X7R expression was examined in neocortex up to 24 h after status epilepticus, in epileptic mice, and in resected neocortex from patients with pharmacoresistent temporal lobe epilepsy (TLE). In addition, the induction of P2X7R after status epilepticus was investigated using transgenic P2X7R reporter mice, which express enhanced green fluorescent protein under the control of the p2x7r promoter.Key FindingsStatus epilepticus resulted in increased P2X7R protein levels in the neocortex of mice. Neocortical P2X7 receptor levels were also elevated in mice that developed epilepsy after status epilepticus and in resected neocortex from patients with pharmacoresistent TLE. Immunohistochemistry determined that neurons were the major cell population transcribing the P2X7R in the neocortex within the first 8 h after status epilepticus, whereas in epileptic mice, P2X7R up‐regulation occurred in microglia as well as in neurons. Pretreatment of mice with the specific P2X7R inhibitor A‐438079 reduced electrographic and clinical seizure severity during status epilepticus and reduced seizure‐induced neuronal death in the neocortex.SignificanceOur findings identify neurons in the neocortex as an important site of P2X7R up‐regulation after status epilepticus and in epilepsy, and provide support for the possible use of P2X7R antagonists for the treatment of status epilepticus and prevention of seizure‐induced brain damage.
Mechanism of dual specificity kinase activity of DYRK1A
Authors: Walte Agnes , Rüben Katharina , Birner‐Gruenberger Ruth , Preisinger Christian , Bamberg‐Lemper Simone , Hilz Nikolaus , Bracher Franz , Becker Walter 
Published: June 2013

The function of many protein kinases is controlled by the phosphorylation of a critical tyrosine residue in the activation loop. Dual specificity tyrosine‐phosphorylation‐regulated kinases (DYRKs) autophosphorylate on this tyrosine residue but phosphorylate substrates on aliphatic amino acids. This study addresses the mechanism of dual specificity kinase activity in DYRK1A and related kinases. Tyrosine autophosphorylation of DYRK1A occurred rapidly during in vitro translation and did not depend on the non‐catalytic domains or other proteins. Expression in bacteria as well as in mammalian cells revealed that tyrosine kinase activity of DYRK1A is not restricted to the co‐translational autophosphorylation in the activation loop. Moreover, mature DYRK1A was still capable of tyrosine autophosphorylation. Point mutants of DYRK1A and DYRK2 lacking the activation loop tyrosine showed enhanced tyrosine kinase activity. A series of structurally diverse DYRK1A inhibitors was used to pharmacologically distinguish different conformational states of the catalytic domain that are hypothesized to account for the dual specificity kinase activity. All tested compounds inhibited substrate phosphorylation with higher potency than autophosphorylation but none of the tested inhibitors differentially inhibited threonine and tyrosine kinase activity. Finally, the related cyclin‐dependent kinase‐like kinases (CLKs), which lack the activation loop tyrosine, autophosphorylated on tyrosine both in vitro and in living cells. We propose a model of DYRK autoactivation in which tyrosine autophosphorylation in the activation loop stabilizes a conformation of the catalytic domain with enhanced serine/threonine kinase activity without disabling tyrosine phosphorylation. The mechanism of dual specificity kinase activity probably applies to related serine/threonine kinases that depend on tyrosine autophosphorylation for maturation.Structured digital abstract

CLK1 and CLK1 phosphorylate by protein kinase assay (View interaction)

HIPK2 and HIPK2 phosphorylate by protein kinase assay (View interaction)

DYRK1A phosphorylates SF3B1 by protein kinase assay (View interaction)

DYRK1A and DYRK1A phosphorylate by protein kinase assay (View interaction)
Sleep Disturbance and Affective Comorbidity Among Episodic Migraineurs
Authors: Walters A. Brooke , Hamer Joshua D. , Smitherman Todd A. 
Published: June 2013

BackgroundDisturbances in sleep are common among migraineurs, particularly those with frequent (ie, chronic) migraine. Examination of specific types of sleep disturbance and behaviors among episodic migraineurs, however, has not been sufficiently explored. Further, few studies have investigated whether sleep disturbance is attributable to comorbid affective symptomatology.ObjectivesThe present case‐control study sought to (1) assess sleep quality, daytime sleepiness, and sleep hygiene among a large sample of episodic migraineurs; (2) quantify relations between sleep disturbance and headache‐related variables; and (3) determine if these relations remain after accounting for comorbid depression and anxiety.MethodsTwo hundred ninety‐two undergraduate students (69.9% female, mean age = 19.19, standard deviation [SD] = 3.21 years) completed measures of sleep quality, daytime sleepiness, and sleep hygiene along with well‐validated measures of depression and anxiety symptomatology. Those screening positive for migraine were subsequently administered a structured diagnostic interview to verify diagnosis of migraine consistent with the International Classification of Headache Disorders, 2nd edition. Episodic migraineurs and non‐migraine controls were compared on the sleep disturbance variables, and among those with migraine, relations with headache frequency, severity, and disability were quantified with linear regression analyses.ResultsSeventy‐eight (26.7%) participants met International Classification of Headache Disorders, 2nd edition criteria for episodic migraine. Compared with participants without migraine, episodic migraineurs reported poorer sleep quality (mean = 8.90 [SD = 3.39] vs 6.63 [SD = 3.02], P < .0001), with 85.9% reporting clinically significant poor sleep quality (vs 62.0% of controls). Poor sleep quality was significantly associated with headache frequency and headache‐related disability, accounting for proportions of variance (14.8% in frequency and 18.2% in disability, both P ≤ .001) similar to those attributable to depression and anxiety. These relationships remained significant after controlling for these affective symptoms, in which sleep quality accounted for 5.3% and 5.8% of unique variance in frequency and disability, respectively (P < .05). By comparison, daytime sleepiness and poor sleep hygiene were not consistently associated with migraine or migraine‐related variables.ConclusionsConsistent with prior studies on chronic migraine, poor sleep quality is uniquely associated with episodic migraine, and this relationship is not solely attributable to comorbid psychiatric symptomatology. Sleep quality should be preferentially assessed (vs sleepiness and sleep hygiene) when subjective self‐report measures of insomnia are used in clinical headache settings. Future studies should supplement these findings by evaluating the efficacy of interventions that specifically target sleep quality and insomnia (eg, stimulus control, sleep restriction) among episodic migraineurs.
The Power of Legal Norms in the EU's External Border Control
Authors: Slominski Peter 
Published: June 2013

AbstractDespite an increased level of legalization of JHA, academic literature has paid little attention to the role of law in this field. It is the objective of this article to assess the EU's attempt to reconcile its current practices of extraterritorial border control coordinated by Frontex in the Mediterranean with international human rights law, notably the principle of non‐refoulement. By drawing on insights on both rationalist and constructivist accounts, we argue that international human rights principles such as non‐refoulement are usually broad enough for everyone to identify and agree with and to provide state actors sufficient leeway to interpret the rules according to their interest. However, thanks to the activities of numerous inter‐, supra‐, and transnational actors offering various and competing legal interpretations, EU member states feel compelled to react by triggering several rounds of rule‐specification that have the power to clarify pertinent law and strengthen fundamental rights standards.
Assessment of Future Climate Change Impact on Water Quality of Chungju Lake, South Korea, Using WASP Coupled with SWAT
Authors: Park Jong Y. , Park Geun A. , Kim Seong J. 
Published: June 2013

AbstractThis study is to evaluate the future potential impact of climate change on the water quality of Chungju Lake using the Water Quality Analysis Simulation Program (WASP). The lake has a storage capacity of 2.75 Gm3, maximum water surface of 65.7 km2, and forest‐dominant watershed of 6,642 km2. The impact on the lake from the watershed was evaluated by the Soil and Water Assessment Tool (SWAT). The WASP and SWAT were calibrated and validated using the monthly water temperatures from 1998 to 2003, lake water quality data (dissolved oxygen, total nitrogen [T‐N], total phosphorus [T‐P], and chlorophyll‐a [chl‐a]) and daily dam inflow, and monthly stream water quality (sediment, T‐N, and T‐P) data. For the future climate change scenario, the MIROC3.2 HiRes A1B was downscaled for 2020s, 2050s, and 2080s using the Change Factor statistical method. The 2080s temperature and precipitation showed an increase of +4.8°C and +34.4%, respectively, based on a 2000 baseline. For the 2080s watershed T‐N and T‐P loads of up to +87.3 and +19.6%, the 2080s lake T‐N and T‐P concentrations were projected to be 4.00 and 0.030 mg/l from 2.60 and 0.016 mg/l in 2000, respectively. The 2080s chl‐a concentration in the epilimnion and the maximum were 13.97 and 52.45 μg/l compared to 8.64 and 33.48 μg/l in 2000, respectively. The results show that the Chungju Lake will change from its mesotrophic state of 2000 to a eutrophic state by T‐P in the 2020s and by chl‐a in the 2080s. Editor's note: This paper is part of a featured series on Korean Hydrology. The series addresses the need for a new paradigm of river and watershed management for Korea due to climate and land use changes.
Evaluation of Operational National Weather Service Gridded Flash Flood Guidance over the Arkansas Red River Basin
Authors: Seo Dugwon , Lakhankar Tarendra , Mejia Juan , Cosgrove Brian , Khanbilvardi Reza 
Published: June 2013

AbstractNational Oceanic and Atmospheric Administration's National Weather Service (NWS) flash flood warnings are issued by Weather Forecast Offices and are underpinned by information from the Flash Flood Guidance (FFG) system operated by the River Forecast Centers (RFCs). This study focuses on the quantitative evaluation and limitations of the FFG system using reported flash flood cases in 2010 and 2011. The flash flood reports were obtained from the NWS Storm Event database for the Arkansas‐Red Basin RFC (ABRFC). The current FFG system at the ABRFC provides gridded flash flood guidance (GFFG) system using the NWS Hydrology Laboratory‐Research Distributed Hydrologic Model to translate the upper zone soil moisture to estimates of Soil Conservation Service Curve Numbers. Comparisons of the GFFG and real‐time Multisensor Precipitation Estimator‐derived Quantitative Precipitation Estimate for the same duration and location were used to analyze the success of the system. Typically, the six‐hour duration was characterized by higher probability of detection values than the three‐hour duration, which highlights the difficulty of hydrologic process estimation for shorter time scales. The current system does not take into account physical characteristics such as land use, including irrigated agricultural farm and urban areas, hence, overly dry soil moisture estimates over these areas can lower the success rate of the GFFG product.
Influence of titanium on in vitro fibroblast–Porphyromonas gingivalis interaction in peri‐implantitis
Authors: Irshad Muhammad , Scheres Nina , Crielaard Wim , Loos Bruno G. , Wismeijer Daniel , Laine Marja L. 
Published: June 2013

AbstractAimTitanium wear particles have been found in peri‐implant tissues, but their role in the pathogenesis of peri‐implantitis remains unclear. We aimed to determine the in vitro inflammatory responses of peri‐implant granulation tissue fibroblasts (PIGFs) to titanium particles alone and in the presence of viable Porphyromonas gingivalis.Materials & MethodsPeri‐implant granulation tissue fibroblasts were challenged either with TiO2 particles, P. gingivalis or a combination of TiO2 particles and P. gingivalis. Gene expression and protein production of pro‐inflammatory mediators by PIGFs were measured with PCR and ELISA, respectively.ResultsHigher doses of TiO2 were toxic to PIGFs and in sub‐toxic doses, TiO2 caused an increase in gene expression of tumour necrosis factor (TNF)‐A and increased protein production of TNF‐α, interleukin (IL)‐6 and IL‐8. A challenge with P. gingivalis alone induced gene expression of TNF‐A, IL‐1β, IL‐6 and IL‐8. A combined challenge with TiO2 and P. gingivalis caused a stronger increase in gene expression of TNF‐A and protein production of TNF‐α and MCP‐1 than P. gingivalis alone.ConclusionsTiO2 particles and P. gingivalis, individually, can induce pro‐inflammatory responses in PIGFs. Furthermore, TiO2 particles and viable P. gingivalis further enhance gene expression and production of TNF‐α by PIGFs. Therefore, Ti wear particles in the peri‐implant tissues in combination with P. gingivalis infection may contribute to the pathogenesis of peri‐implantitis by enhancing the inflammation in peri‐implant tissues.
Influence of periodontal disease, Porphyromonas gingivalis and cigarette smoking on systemic anti‐citrullinated peptide antibody titres
Authors: Lappin David F. , Apatzidou Danae , Quirke Anne‐Marie , Oliver‐Bell Jessica , Butcher John P. , Kinane Denis F. , Riggio Marcello P. , Venables Patrick , McInnes Iain B. , Culshaw Shauna 
Published: June 2013

AbstractBackgroundAnti‐citrullinated protein antibody (ACPA) responses may precede clinical onset of rheumatoid arthritis. Porphyromonas gingivalis peptidylarginine deiminase can citrullinate proteins possibly inducing autoimmunity in susceptible individuals.AimTo determine whether periodontitis, carriage of P. gingivalis, smoking and periodontal therapy influence ACPA titres.MethodsSerum and plaque samples were collected from 39 periodontitis patients before and after non‐surgical periodontal treatment, and from 36 healthy subjects. Carriage of P. gingivalis was determined by PCR of plaque DNA. ACPA was determined by anti‐cyclic citrullinated peptide (CCP) enzyme‐linked immunosorbent assay (ELISA). Anti‐P. gingivalis titres were determined by ELISA.ResultsUntreated periodontitis patients had higher anti‐CCP antibody titres than healthy controls [three patients (8%) greater than manufacturer suggested assay diagnostic threshold (5 Assay Units/AU) versus none (0%); mean ± SEM: 1.37 ± 0.23 versus 0.40 ± 0.10 AU, p < 0.0001]. Periodontitis patients who smoked demonstrated lower anti‐P. gingivalis (15956 ± 4385 versus 2512 ± 1290 Units/ml, p < 0.05), but similar anti‐CCP than non‐smoking periodontitis patients (smokers: 1.31 ± 0.35; non‐smokers: 1.41 ± 0.32 AU). Healthy smokers demonstrated elevated anti‐CCP titres (0.75 ± 0.19 AU), at levels between healthy non‐smokers (0.15 ± 0.05 AU) and non‐smoker periodontitis patients. Six months after periodontal treatment, there were significant reductions in anti‐CCP (non‐smokers p < 0.05) and anti‐P. gingivalis (all participants p < 0.01).ConclusionIn subjects with periodontitis, P. gingivalis infection may be responsible for inducing autoimmune responses that characterize rheumatoid arthritis.