bluuebunny/crossref_metadata_2025_split huggingface dataset:
RRP41L, a Putative Core Subunit of the Exosome, Plays an Important Role in Seed Germination and Early Seedling Growth in Arabidopsis
Authors: Yang Min , Zhang Bangyue , Jia Jianheng , Yan Chunxia , Habaike Ayijiang , Han Yuzhen 
Published: November 2012

AbstractIn prokaryotic and eukaryotic cells, the 3′-5′-exonucleolytic decay and processing of RNAs are essential for RNA metabolism. However, the understanding of the mechanism of 3′-5′-exonucleolytic decay in plants is very limited. Here, we report the characterization of an Arabidopsis (Arabidopsis thaliana) transfer DNA insertional mutant that shows severe growth defects in early seedling growth, including delayed germination and cotyledon expansion, thinner yellow/pale-green leaves, and a slower growth rate. High-efficiency thermal asymmetric interlaced polymerase chain reaction analysis showed that the insertional locus was in the sixth exon of AT4G27490, encoding a predicted 3′-5′-exonuclease, that contained a conserved RNase phosphorolytic domain with high similarity to RRP41, designated RRP41L. Interestingly, we detected highly accumulated messenger RNAs (mRNAs) that encode seed storage protein and abscisic acid (ABA) biosynthesis and signaling pathway-related protein during the early growth stage in rrp41l mutants. The mRNA decay kinetics analysis for seed storage proteins, 9-cis-epoxycarotenoid dioxygenases, and ABA INSENSITIVEs revealed that RRP41L catalyzed the decay of these mRNAs in the cytoplasm. Consistent with these results, the rrp41l mutant was more sensitive to ABA in germination and root growth than wild-type plants, whereas overexpression lines of RRP41L were more resistant to ABA in germination and root growth than wild-type plants. RRP41L was localized to both the cytoplasm and nucleus, and RRP41L was preferentially expressed in seedlings. Altogether, our results showed that RRP41L plays an important role in seed germination and early seedling growth by mediating specific cytoplasmic mRNA decay in Arabidopsis.
The Bemisia tabaci species complex: Additions from different parts of the world
Authors: Firdaus Syarifin , Vosman Ben , Hidayati Nurul , Jaya Supena Ence Darmo , G.F. Visser Richard , van Heusden Adriaan Willem 
Published: November 2012

AbstractBemisia tabaci is one of the most threatening pests in many crops. We sequenced part of the mitochondrial cytochrome oxidase I gene from fifty whitefly populations collected in Indonesia, Thailand, India and China. Nineteen unique sequences (haplotypes) of the cytochrome oxidase I were identified in these populations. They were combined with sequences available in databases, resulting in a total of 407 haplotypes and analyzed together with nine outgroup accessions. A phylogenetic tree was calculated using the maximum likelihood method. The tree showed that all groups that were found in previous studies were also present in our study. Additionally, seven new groups were identified based on the new haplotypes. Most B. tabaci haplotypes grouped based on their geographical origin. Two groups were found to have a worldwide distribution. Our results indicate that our knowledge on the species complex around B. tabaci is still far from complete.
Protection from Procedural Myocardial Injury by Omega‐3 Polyunsaturated Fatty Acids (PUFAs): Is Related with Lower Levels of Creatine Kinase‐MB (CK‐MB) and Troponin I?
Authors: Foroughinia Farzaneh , Salamzadeh Jamshid , Namazi Mohammad H. 
Published: November 2012

SummaryObjectiveThis study sought to investigate the effect of omega‐3 polyunsaturated fatty acids (PUFAs) on cardiac biomarkers, CK‐MB, and troponin I in patients undergoing PCI.BackgroundRestenosis remains as a major long‐term complication following percutaneous coronary intervention (PCI). It appears that there is strong relationship between post‐PCI creatine kinase‐MB (CK‐MB) and troponin I elevation and cardiovascular events after PCI.MethodsIn this randomized clinical trial, a total of 90 patients planned to undergo PCI were randomly assigned into two groups: Group A–receiving omega‐3 PUFAs (3 g, 12 h before PCI) plus standard treatment (n = 43) and Group B–control group, receiving only standard therapy (n = 47). Standard treatment included aspirin 325 mg and clopidogrel 600 mg loading dose. The plasma CK‐MB level was measured before the procedure (baseline), at 8 and 24 h after PCI. The plasma troponin I was measured at baseline and 24 h after PCI.ResultsIn comparison with control, omega‐3 PUFAs could significantly reduce the level of CK‐MB in 8 (P = 0.001) and 24 h (P = 0.012) after its prescription in the omega‐3 PUFAs group. Omega‐3 PUFAs could not significantly decrease troponin I.ConclusionOur results revealed that omega‐3 PUFAs can be considered as a safe adjunctive medication to the standard regimen before PCI for the aim of decreasing cardiovascular event after PCI.
Archie Brain: celebrating 30 years of development in laryngeal mask airways
Authors: van Zundert T. C. R. V. , Brimacombe J. R. , Ferson D. Z. , Bacon D. R. , Wilkinson D. J. 
Published: November 2012

SummaryThe practice of anaesthesia was revolutionised by the ideas of Archie Brain. The routine use of a facemask to manage the airway was not a hands‐free technique, despite the development of various harnesses, and made adequate record‐keeping difficult. The tracheal tube was associated with some morbidity, which some felt was unsuitable for day surgery. Brain developed an airway management device that was less stressful to the patient than tracheal intubation, and was, however, as safe as using a facemask and airway. Brain also hoped his device would function for cases where mask ventilation was particularly difficult and thus give anaesthetists a safer alternative to a complex intubation, especially in emergency scenarios.
Comparative ultrastructure of the spermatozoa of the Majoidea (Crustacea, Decapoda, Brachyura) with new data on six species in five genera
Authors: Tudge Christopher C. , Scheltinga  David M. , Jamieson  Barrie G. M. , Guinot  Danièle , Richer de Forges Bertrand 
Published: November 2012

AbstractComparative ultrastructure of majoid spermatozoa belonging to 23 species, in 19 genera and five families, is considered, with new data on Schizophrys aspera; S. rufescens (Majidae, Majinae); Camposcia retusa (Inachidae); Pyromaia tuberculata (Inachoididae); and Huenia heraldica and Menaethius monoceros (Epialtidae, Epialtinae). The oregoniid Chionoecetes opilio, and inachids Cyrtomaia furici, Platymaia rebierei, Macropodia longirostris and Inachus phalangium, possibly with Camposcia retusa, but not Podochela riisei, appear to form a group. Within the inachids, Macropodia and Inachus are especially close. A domed central acrosome zone, seen in most inachid sperm, in majines (both Schizophrys species), in pisines (Oxypleurodon orbiculatus and O. stuckiae) and epialtines (Huenia heraldica and Menaethius monoceros), appears to be an autapomorphy of these majoids. A peripheral acrosome zone is seen in the inachid Grypacheus hyalinus, two inachoidids (P. tuberculata and Stenorhynchus seticornis) and the majid Maja squinado. Pyromaia tuberculata differs from other inachoidids in having a slightly dome‐shaped operculum. The mithracine Macrocoeloma trispinosum (Majidae) sperm more closely resembles Inachoididae, than Inachidae. Spermatologically, the family Majidae and the subfamily Majinae are not homogeneous. Spermatozoal ultrastructure does not support a majoid–hymenosomatid relationship and is equivocal with regard to the placement of Cryptochiridae in either the Thoracotremata or Heterotremata, the prominent operculum strongly differentiates cryptochirids from Majoidea.
3D numerical modelling of graben interaction and linkage: a case study of the Canyonlands grabens, Utah
Authors: Allken Vaneeda , Huismans Ritske S. , Fossen Haakon , Thieulot Cedric 
Published: November 2012

AbstractGraben systems in extensional settings tend to be segmented with evidence of segment interaction. To gain a better understanding of the evolution of structures formed during graben growth and interaction, we here study the Grabens area of Canyonlands National Park, Utah, where a wide range of such structures is well exposed. With the aid of 3D numerical models, we attempt to reproduce structures observed in that region and to understand controls on the structural style of graben interaction by varying the spacing between pre‐existing structures. The sensitivity of the system to the thickness of the salt layer is also tested. Four distinct types of structures are observed when the spacing between inherited weak zones is varied: (1) grabens connecting in a relay zone divided by a narrow central horst; (2) graben segments interacting via a secondary stepover graben; (3) grabens propagating alongside each other with limited segment interaction; and (4) an abandoned graben segment in a system of multiple competing grabens. The presence of a basal salt layer (Paradox Member) promotes efficient graben propagation. A comparison between the observed structures and the numerical model results indicates that the detachment salt layer is relatively thin in the study area.
Benefits of progestin contraception in non‐allergic angioedema
Authors: Saule C. , Boccon‐Gibod I. , Fain O. , Kanny G. , Plu‐Bureau G. , Martin L. , Launay D. , Bouillet L. , Gompel A. 
Published: November 2012

SummaryBackgroundHereditary angioedema attacks can be induced or worsened by oral contraceptive containing oestrogens.ObjectivesThe purpose of this study was to assess the impact of progestin contraceptives on angioedema attacks.MethodsWe conducted a French retrospective, multi‐centre study of progestin contraception in women with non‐allergic angioedema, including hereditary angioedema type I, II and III and idiopathic angioedema. Patients were classified into four groups according to frequency of attacks. We evaluated the effects of progestin on the mean number of attacks and compared the number of patients in each group before and under progestin contraception. The influence of hormonal factors on the course of angioedema was also assessed.ResultsFifty‐five women were included: mean age was 32.1 years (16–52) and mean follow‐up 32.4 months (SD:29). Fourteen women were classified as type I (25.4%), two as type II (3.6%) and 19 as type III (34%) and 20 were idiopathic (36%). Seventeen patients were taking a low dose progestin‐only pill (POP), 24 antigonadotropic progestins (AGP) and 14 both successively. Total or partial improvement was observed in 81.8% (45/55) of the patients and more frequently in those on an AGP agent (34 patients, 89.5%) than on POP (19 patients, 61.3%) (P = 0.013).Conclusions & Clinical RelevanceThis is the first study evaluating the interest of antigonadotropic progestin contraception in a series of women with non‐allergic angioedema. Progestins, especially antigonadotropic progestins, appear to convey a marked benefit in most cases. Antigonadotropic progestins could thus be recommended as adjuvant treatment in childbearing women with non‐allergic angioedema.
Bifid mandibular canals and the factors associated with their presence: a medical computed tomography evaluation in a Taiwanese population
Authors: Fu Earl , Peng Michelle , Chiang Cheng‐Yang , Tu Hsiao‐Pei , Lin Yaoh‐Shiang , Shen E‐Chin 
Published: November 2012

AbstractObjectivesTo examine distribution of bifid mandibular canals in a Taiwanese population and to evaluate factors contributing to the phenomenon.Material and methodsComputed tomographic images from 173 subjects (97 females and 76 males) were obtained using a 64‐slice multidetector computerized tomography system, and the presence of bifid mandibular canals, as well as their widths and lengths, was examined. Association of length of bifid canals with possible contributing factors, including gender, age, and side of presentation, as well as size of cross‐sectional bony area of mandible along the long axis of mandibular canal, was evaluated.ResultsBifid mandibular canals, with mean values of 10.1 and 0.9 mm in length and width, were found in 53 (30.6%) of 173 patients and 64 (18.5%) of 346 hemi‐mandibles. Bifid canals appeared more frequently and tend to penetrate mandible with greater lengths in males if compared with those in females. When males were compared with females and when mandibles with bifid canals were compared with ones without, the former tend to present with larger bony area at corresponding levels of cross‐sectional plane than the later, respectively. By regression analysis, significant association was found between length of bifid canals and gender, side of hemi‐mandible, and bony area at mid‐zone of mandibular canal.ConclusionsBifid canals were observed in 30.6% of subjects and 18.5% of hemi‐mandibles. Significant association between length of bifid canals and gender, side of hemi‐mandible, and cross‐sectional bony area of mandible was observed.
Early extensive viremia, but not rs8099917 genotype, is the only predictor for cholestatic hepatitis C after living‐donor liver transplantation
Authors: Ikegami Toru , Shirabe Ken , Fukuhara Takasuke , Furusyo Norihiro , Kotoh Kazuhiro , Kato Masaki , Shimoda Shinji , Aishima Shinichi , Soejima Yuji , Yoshizumi Tomoharu , Maehara Yoshihiko 
Published: November 2012

AimCholestatic hepatitis C is one of the most serious but still unaddressed disorders after liver transplantation.MethodsIn this study, we analyzed 49 patients who underwent living‐donor liver transplantation (LDLT) to treat hepatitis C virus (HCV) infection.ResultsFive patients developed cholestatic hepatitis C, with total bilirubin of 15.2 ± 3.1 mg/dL at diagnosis 6.2 ± 1.0 weeks after LDLT. Univariate analysis showed that larger graft to standard liver volume ratio, higher HCV RNA titer at 2 weeks, earlier peak HCV RNA titer and cytomegalovirus infection were the significant risk factors. The development of cholestatic hepatitis C was not significantly associated with interleukin‐28B genotype (rs8099917); four out of five affected patients had the T/T genotype. Multivariate analysis showed that higher HCV RNA titer at 2 weeks was the only significant factor (P = 0.026) for the development of cholestatic hepatitis C. Receiver–operator curve analysis showed that that HCV RNA titer of more than 7.2 log10IU/mL was the optimal cut‐off for characterizing cholestatic hepatitis C. All of the patients were serum HCV RNA negative after treatment with pegylated interferon and ribavirin and all the patients are alive.ConclusionEarly extensive viremia, but not the rs8099917 genotype, was the only predictor for cholestatic hepatitis C after LDLT.
Expression of c‐Jun and Sox‐2 in human schwannomas and traumatic neuromas
Authors: Shivane Aditya , Parkinson David B , Ammoun Sylwia , Hanemann Clemens O 
Published: November 2012

AimsSchwann cells myelinate axons of the peripheral nervous system. This process of myelination is regulated by various transcription factors. c‐Jun and Sox‐2 are negative regulators of myelination and control Schwann cell differentiation and plasticity. Schwannoma cells within tumours no longer express myelin markers, and show increased proliferation and decreased apoptosis. We have shown previously that several signalling pathways are activated in schwannoma cells in situ, in particular the c‐Jun N‐terminal kinase (JNK) pathway. Both in vitro and in vivo we have demonstrated that c‐Jun and Sox‐2 are co‐regulated in Schwann cells and evidence shows that both these proteins regulate myelination negatively. In this study, we aimed to characterize the expression of c‐Jun and Sox‐2 in schwannoma and traumatic neuroma.Methods and resultsImmunohistochemistry using antibodies to c‐Jun and Sox‐2 was applied to six schwannomas, and the results were compared with those seen in traumatic neuroma and normal nerve. Increased expression of c‐Jun and Sox‐2 was seen in schwannoma.ConclusionsWe have demonstrated increased expression of c‐Jun and Sox‐2 in schwannoma compared to traumatic neuroma. There was no expression of c‐Jun and Sox‐2 in a histologically normal peripheral nerve.
Suitability of 10 Hz vertical geophones for seismic noise array measurements based on frequency‐wavenumber and extended spatial autocorrelation analyses
Authors: Rosa‐Cintas S. , Galiana‐Merino J.J. , Rosa‐Herranz J. , Molina S. , Giner‐Caturla J. 
Published: November 2012

ABSTRACTMicrozonation studies using ambient noise measurements constitute a promising way for seismic hazard evaluation in urban areas. Among the existing techniques, seismic noise array measurements have become a valuable tool for estimating Vs profiles and thus, the characteristics of a soil structure. Although methods based on analysis of seismic noise are simpler, cheaper and faster than borehole drilling and down‐hole or cross‐hole logs to derive shear‐wave velocity profiles, array deployment requires the use of several stations (broadband or short‐period sensors) that are not always available. In this paper, we have compared the results obtained by 10 Hz‐vertical‐geophone arrays with the results provided by 1 Hz‐sensor arrays. Two sites in the Bajo Segura Basin (SE Spain), with different soil characteristics, were chosen for array deployment. The comparison is carried out in terms of dispersion curves by using frequency‐wavenumber (f‐k) and extended spatial autocorrelation (ESAC) techniques. Both analyses show a good agreement using either 1 Hz sensors or 10 Hz geophones; moreover, they demonstrate that it is possible to extend the analysis in a frequency range much below the natural frequency of the geophones. The results of our study confirm the suitability of standard seismic refraction/reflection equipment also for ambient noise array measurements, which constitutes a cheaper and faster way for investigating soil characteristics.
Absorption related velocity dispersion below a possible gas hydrate geobody
Authors: Jones Ian F. 
Published: November 2012

ABSTRACTVelocity dispersion is not usually a problem in surface seismic data processing, as the seismic bandwidth is relatively narrow and thus for most Q values, dispersive effects are not noticeable. However, for highly absorptive bodies, such as the overpressured free gas accumulations associated with some gas hydrates or high‐porosity normally pressured gas sands, dispersive effects may be seen. In this work I analyse one such data set from the offshore north‐east coast of India. I demonstrate that the effect is measurable and that compensating for it in either data processing or migration can improve the wavelet character, as well as delivering an estimate of the effective Q values in the associated geobody. I also raise the question as to whether velocities derived using low‐frequency waveform inversion over such dispersive geobodies are wholly appropriate for migration of full seismic‐bandwidth data.
High hydrostatic pressure (HHP) inactivation of foodborne pathogens in low‐acid juices
Authors: Pilavtepe‐Çelik Mutlu 
Published: November 2012

SummaryConsumption of unpasteurised fruit/vegetable juices has increased in recent years due to their freshness, low calorie contribution and good nutritional quality. However, unpasteurised fresh juices with low acidity (pH > 4.6) and high water activity (aw > 0.85) can support the growth of pathogens. Hence, pasteurisation is a necessary process in the production of low‐acid juices. Consumer demand has required minimally processed high‐quality foods that are free from additives, that are fresh tasting and microbiologically safe, and with an extended shelf life. High hydrostatic pressure (HHP) treatment is considered to be an alternative to thermal pasteurisation for fruit and vegetable juices. HHP treatment could preserve nutritional value and the sensory properties of fruits and vegetables due to its limited effect on the covalent bonds of low‐molecular‐mass compounds such as colour, flavour compounds and vitamins. However, inactivation of important foodborne pathogens in low‐acid foods by HHP is most urgent and critical. More research should be performed in order to satisfy consumer demands for fresh‐tasting products while retaining safety.
Functional properties of soy sauce and metabolism genes of strains for fermentation
Authors: Zhao Guozhong , Yao Yunping , Wang Xiaohua , Hou Lihua , Wang Chunling , Cao Xiaohong 
Published: November 2012

SummarySoy sauce is considered the most important food condiment in China for its distinct fragrance and salty taste. Highly valued for its unique tastes and flavours, soy sauce exhibits many beneficial functions, such as 4‐hydroxy‐2(or 5)‐ethyl‐5(or 2)‐methyl‐3(2H)‐furanone and 4‐ethylguaiacol. Soy sauce has been widely used in Asian countries for cooking. With the rapidly growing popularity of these unique tastes and flavours, it is important to have a comprehensive reference of its functional benefits. Meanwhile, the genes encoding the major enzymes of metabolism exhibit specialised roles. This review provides a valuable source for the abundant metabolism genes of strains and current knowledge on antioxidant activity, antihypertensive effect, anticarcinogenic activity, fortification and their relationships with soy sauce tastes and flavours.
Odour‐active compounds in pineapple (Ananas comosus [L.] Merril cv. Red Spanish)
Authors: Pino Jorge A. 
Published: November 2012

SummaryApplication of solid‐phase microextraction, simultaneous distillation–extraction and liquid–liquid extraction combined with GC‐FID, GC‐MS, aroma extract dilution analysis, and odour activity value was used to analyse volatile compounds from pineapple (Ananas comosus [L.] Merril cv. Red Spanish) and to estimate the most odour‐active compounds. The analyses led to the identification of ninety‐four compounds, seventy‐two of them were positively identified. Twenty odorants were considered as odour‐active compounds, from which ethyl 2‐methylbutanoate, 2,5‐dimethyl‐4‐hydroxy‐3(2H)‐furanone, 1‐(E,Z,Z)‐3,5,8‐undecatetraene, ethyl 3‐(methylthio)propanoate, 1‐(E,Z)‐3,5‐undecatriene, ethyl hexanoate and methyl hexanoate were the most odour contributors and contribute to the typical pineapple aroma, while the others are responsible for fruity and sweet odour notes.
Gene therapy for lower urinary tract dysfunction
Authors: Yoshimura Naoki , Miyazato Minoru , Sasaki Katsumi , Yokoyama Hitoshi , Oguchi Tomohiko , Chancellor Michael B , Funahashi Yasuhito 
Published: November 2012

AbstractLower urinary tract dysfunction is caused by functional and pathophysiological alterations of the peripheral organs, including the urothelium and detrusor smooth muscle, as well as peripheral and central nervous systems. Recent research in this field has increased our understanding of the mechanisms of lower urinary tract dysfunction, and new drugs have been developed, leading to increased treatment options and changing medical care for lower urinary tract symptoms. Nevertheless, clinicians still often experience refractory and treatment‐resistant cases against conventional therapeutic modalities. For such cases, gene therapy targeting for the lower urinary tract and its afferent pathway is anticipated to offer a new therapeutic approach. Therefore, in this article, we review the possibility and current status of gene therapy for lower urinary tract dysfunction.