bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of obesity on the survival of patients with early‐stage squamous cell carcinoma of the oral tongue
Authors: Iyengar Neil M. , Kochhar Amit , Morris Patrick G. , Morris Luc G. , Zhou Xi K. , Ghossein Ronald A. , Pino Alejandro , Fury Matthew G. , Pfister David G. , Patel Snehal G. , Boyle Jay O. , Hudis Clifford A. , Dannenberg Andrew J. 
Published: January 2014

BACKGROUNDAlthough obesity increases risk and negatively affects survival for many malignancies, the prognostic implications in squamous cell carcinoma (SCC) of the oral tongue, a disease often associated with prediagnosis weight loss, are unknown.METHODSPatients with T1‐T2 oral tongue SCC underwent curative‐intent resection in this single‐institution study. All patients underwent nutritional assessment prior to surgery. Body mass index (BMI) was calculated from measured height and weight and categorized as obese (≥ 30 kg/m2), overweight (25‐29.9 kg/m2), or normal (18.5‐24.9 kg/m2). Clinical outcomes, including disease‐specific survival, recurrence‐free survival, and overall survival, were compared by BMI group using Cox regression.RESULTSFrom 2000 to 2009, 155 patients (90 men, 65 women) of median age 57 years (range, 18‐86 years) were included. Baseline characteristics were similar by BMI group. Obesity was significantly associated with adverse disease‐specific survival compared with normal weight in univariable (hazard ratio [HR] = 2.65, 95% confidence interval [CI] = 1.07‐6.59; P = .04) and multivariable analyses (HR = 5.01; 95% CI = 1.69‐14.81; P = .004). A consistent association was seen between obesity and worse recurrence‐free survival (HR = 1.87; 95% CI = 0.90‐3.88) and between obesity and worse overall survival (HR = 2.03; 95% CI = 0.88‐4.65) though without reaching statistical significance (P = .09 and P = .10, respectively) in multivariable analyses.CONCLUSIONSIn this retrospective study, obesity was an adverse independent prognostic variable. This association may not have been previously appreciated due to confounding by multiple factors including prediagnosis weight loss. Cancer 2014;120:983–991. © 2013 American Cancer Society.
Two‐Color RESOLFT Nanoscopy with Green and Red Fluorescent Photochromic Proteins
Authors: Lavoie‐Cardinal Flavie , Jensen Nickels A. , Westphal Volker , Stiel Andre C. , Chmyrov Andriy , Bierwagen Jakob , Testa Ilaria , Jakobs Stefan , Hell Stefan W. 
Published: January 2014

AbstractUp to now, all demonstrations of reversible saturable optical fluorescence transitions (RESOLFT) superresolution microscopy of living cells have relied on the use of reversibly switchable fluorescent proteins (RSFP) emitting in the green spectral range. Here we show RESOLFT imaging with rsCherryRev1.4, a new red‐emitting RSFP enabling a spatial resolution up to four times higher than the diffraction barrier. By co‐expressing green and red RSFPs in living cells we demonstrate two‐color RESOLFT imaging both for single (“donut”) beam scanning and for parallelized versions of RESOLFT nanoscopy where an array of >23 000 “donut‐like” minima are scanned simultaneously.
Synthesis of C8‐Functionalized Magnetic Graphene with a Polydopamine Coating for the Enrichment of Low‐Abundance Peptides
Authors: Zhao Man , Deng Chunhui , Zhang Xiangmin 
Published: January 2014

AbstractFor the first time, C8‐functionalized magnetic graphene (magG) has been designed and synthesized with a polydopamine (PDA) coating. The magG prepared by a solvothermal reaction was encapsulated in a layer of PDA through the oxidative polymerization of dopamine in alkaline buffer, and C8 groups were grafted onto magG/PDA composites through a silanization method. The as‐prepared material integrates the merits of graphene, magnetic microspheres, PDA, and C8 chains; thus possessing an ultrahigh specific area, strong magnetic responsiveness, excellent solubility, and an extraordinary enrichment capability. The C8‐functionalized magnetic composites were employed in the enrichment and identification of low‐concentration standard peptides, peptides in standard protein digest solutions, and endogenous peptides in human urine. The enriched peptides were eluted and analyzed by MALDI‐TOF MS. The MS results indicated that the C8‐functionalized material exhibited the distinguished ability to enrich hydrophobic peptides mainly through hydrophobic–hydrophobic interactions. Moreover, thanks to the enrichment approach based on magG@PDA@C8, the limit of detection of the standard peptide decreased to as low as 50 pM. The experimental results demonstrate that the magG@PDA@C8 composite is a promising candidate for the enrichment of low‐abundance peptides in biological samples.
Water Oxidation by Amorphous Cobalt‐Based Oxides: Volume Activity and Proton Transfer to Electrolyte Bases
Authors: Klingan Katharina , Ringleb Franziska , Zaharieva Ivelina , Heidkamp Jonathan , Chernev Petko , Gonzalez‐Flores Diego , Risch Marcel , Fischer Anna , Dau Holger 
Published: January 2014

AbstractWater oxidation in the neutral pH regime catalyzed by amorphous transition‐metal oxides is of high interest in energy science. Crucial determinants of electrocatalytic activity were investigated for a cobalt‐based oxide film electrodeposited at various thicknesses on inert electrodes. For water oxidation at low current densities, the turnover frequency (TOF) per cobalt ion of the bulk material stayed fully constant for variation of the thickness of the oxide film by a factor of 100 (from about 15 nm to 1.5 μm). Thickness variation changed neither the nanostructure of the outer film surface nor the atomic structure of the oxide catalyst significantly. These findings imply catalytic activity of the bulk hydrated oxide material. Nonclassical dependence on pH was observed. For buffered electrolytes with pKa values of the buffer base ranging from 4.7 (acetate) to 10.3 (hydrogen carbonate), the catalytic activity reflected the protonation state of the buffer base in the electrolyte solution directly and not the intrinsic catalytic properties of the oxide itself. It is proposed that catalysis of water oxidation occurs within the bulk hydrated oxide film at the margins of cobalt oxide fragments of molecular dimensions. At high current densities, the availability of a proton‐accepting base at the catalyst–electrolyte interface controls the rate of water oxidation. The reported findings may be of general relevance for water oxidation catalyzed at moderate pH by amorphous transition‐metal oxides.
Enhanced Photoelectrochemical Water Splitting Efficiency of a Hematite–Ordered Sb:SnO2 Host–Guest System
Authors: Wang Lei , Palacios‐Padrós Anna , Kirchgeorg Robin , Tighineanu Alexei , Schmuki Patrik 
Published: January 2014

AbstractHost–guest systems such as hematite/SnO2 have attracted a great deal of interest as photoanodes for photoelectrochemical water splitting. In the present work we form an ordered porous tin oxide layer formed by self‐organizing anodization of Sn films on a FTO substrate. Subsequently the anodic tin oxide nanostructure is doped with antimony (ATO) by a simple impregnation and annealing treatment, and then decorated with hematite using anodic deposition. Photoelectrochemical water splitting experiments show that compared to conventional SnO2 nanostructures, using a Sb doped nanochannel SnO2 as a host leads to a drastic increase of the water splitting photocurrent response up to 1.5 mA cm−2 at 1.6 V (vs. RHE) in 1 M KOH under AM 1.5 (100 mW cm−2) conditions compared to 0.04 mA cm−2 for the non‐Sb doped SnO2 scaffold.
Relationship Between Blood Levels and the Anti‐Hyperalgesic Effect of Ketoprofen in the Rat
Authors: Aguilar‐Carrasco José Carlos , Rodríguez‐Silverio Juan , Jiménez‐Andrade Juan Miguel , Carrasco‐Portugal Miriam del Carmen , Flores‐Murrieta Francisco Javier 
Published: January 2014

Abstract

Preclinical Research

The relationship between blood levels of ketoprofen and its anti‐hyperalgesic effects was examined in rat using the carrageenan‐evoked thermal hyperalgesia model. Female adult Wistar rats were injected with carrageenan into the plantar surface of the right hind paw. Immediately after, rats were administered with ketoprofen po and hindpaw withdrawal latency measured and micro‐whole blood samples were obtained over six hours via a cannula inserted in the caudal artery. Ketoprofen levels were measured by HPLC. Ketoprofen concentration increased in a dose‐dependent manner and was reflected in dose‐dependent anti‐hyperalgesic effect. The pharmacokinetic and pharmacodynamic parameters expressed as mean ± s.e.m. following administration of 1, 3.2, and 10 mg/kg ketoprofen were: Cmax 1.27 ± 0.08, 3.44 ± 0.20 and 11.76 ± 0.81 μg/mL; AUClast 4.16 ± 0.17, 11.63 ± 0.65 and 28.15 ± 1.32 μg h/mL; and Emax observed (AUCE): 65.41 ± 7.79, 92.06 ± 6.46 and 98.42 ± 7.53%. A direct relationship between blood concentrations and the anti‐hyperalgesic effect of ketoprofen followed a maximum effect model equation. The results indicate that the anti‐hyperalgesic effect of ketoprofen in the carrageenan pain model can be predicted by the pharmacokinetic properties of ketoprofen.
Process Evaluation of the MOSAIC Trial, Part I: Therapist Experiences of Delivering Two Psychological Therapies for Treatment of Anorexia Nervosa
Authors: Waterman‐Collins Daniella , Renwick Beth , Lose Anna , Kenyon Martha , Serpell Lucy , Richards Lorna , Boughton Nicky , Treasure Janet , Schmidt Ulrike ,   on behalf of the MOSAIC trial group
Published: January 2014

AbstractObjectivesForming part of a process evaluation of a large randomised controlled trial (the Maudsley Outpatient Study of Treatments for Anorexia Nervosa and Related conditions, MOSAIC) comparing two outpatient therapies for Anorexia Nervosa (AN), the Maudsley Model for Treatment of Adults with Anorexia Nervosa (MANTRA) and Specialist Supportive Clinical Management (SSCM), this study adopted a qualitative approach to examine therapist experiences of treatment delivery.MethodTwenty MOSAIC therapists completed semi‐structured interviews. Interviews were recorded, transcribed and analysed thematically.ResultsThemes of positive aspects, challenges and therapeutic fit emerged. MANTRA was seen as structured and flexible but could feel demanding on therapist time and skill. The slow pace and narrower focus of SSCM gave patients space to talk, but the lack of psychological tools and nutritional emphasis could create frustration. Views on the therapeutic relationship and patient‐therapy fit differed across treatments.DiscussionFindings provide testable hypotheses about what works for whom, ideas for therapist training, treatment development and delivery. Copyright © 2014 John Wiley & Sons, Ltd and Eating Disorders Association.
The effects of cultural differences between the west and Saudi Arabia on emergency evacuation—clothing effects on walking speed
Authors: Almejmaj Majed , Meacham Brian , Skorinko Jeanine 
Published: January 2014

SummaryEvacuation analysis is an integral part of safety when dealing with the increasing number of complex building designs around the world. During such analysis, engineers use predetermined input values based on experiments for factors such as alarm reaction time and walking speed. However, much of the data currently in use were collected in Western countries, such as the UK and the USA, and were applied regardless of where the building will be constructed. This research explores the suitability of applying such data cross‐culturally, with a focus on Saudi Arabia. Saudi Arabia is of particular interest because of cultural differences in traditional clothing, personal space, fire training, and religious requirements, such as gender segregation. Because these factors could impact evacuation behavior and movement speeds, data were collected and analyzed on the effects of traditional Saudi clothing on movement speeds of different genders in Saudi Arabia. Outcomes of our analysis indicate that the length and type of clothing worn by women in Saudi Arabia have a significant effect on their walking speeds on flat surfaces and when ascending and descending stairways. This paper highlights the effects of cultural differences and presents a set of data that can be used when conducting an evacuation analysis within Saudi Arabia. Copyright © 2014 John Wiley & Sons, Ltd.
Interaction of Integrin β4 With S1P Receptors in S1P‐ and HGF‐Induced Endothelial Barrier Enhancement
Authors: Ni Xiuqin , Epshtein Yulia , h  , Chen Weiguo , Zhou Tingting , Xie Lishi , Garcia Joe G.N. , Jacobson Jeffrey R. 
Published: January 2014

ABSTRACTWe previously reported sphingosine 1‐phosphate (S1P) and hepatocyte growth factor (HGF) augment endothelial cell (EC) barrier function and attenuate murine acute lung inury (ALI). While the mechanisms underlying these effects are not fully understood, S1P and HGF both transactivate the S1P receptor, S1PR1 and integrin β4 (ITGB4) at membrane caveolin‐enriched microdomains (CEMs). In the current study, we investigated the roles of S1PR2 and S1PR3 in S1P/HGF‐mediated EC signaling and their associations with ITGB4. Our studies confirmed ITGB4 and S1PR2/3 are recruited to CEMs in human lung EC in response to either S1P (1 µM, 5 min) or HGF (25 ng/ml, 5 min). Co‐immunoprecipitation experiments identified an S1P/HGF‐mediated interaction of ITGB4 with both S1PR2 and S1PR3. We then employed an in situ proximity ligation assay (PLA) to confirm a direct ITGB4–S1PR3 association induced by S1P/HGF although a direct association was not detectable between S1PR2 and ITGB4. S1PR1 knockdown (siRNA), however, abrogated S1P/HGF‐induced ITGB4–S1PR2 associations while there was no effect on ITGB4–S1PR3 associations. Moreover, PLA confirmed a direct association between S1PR1 and S1PR2 induced by S1P and HGF. Finally, silencing of S1PR2 significantly attenuated S1P/HGF‐induced EC barrier enhancement as measured by transendothelial resistance while silencing of S1PR3 significantly augmented S1P/HGF‐induced barrier enhancement. These results confirm an important role for S1PR2 and S1PR3 in S1P/HGF‐mediated EC barrier responses that are associated with their complex formation with ITGB4. Our findings elucidate novel mechanisms of EC barrier regulation that may ultimately lead to new therapeutic targets for disorders characterized by increased vascular permeability including ALI. J. Cell. Biochem. 115: 1187–1195, 2014. © 2013 Wiley Periodicals, Inc.
MicroRNA‐190 Regulates Hypoxic Pulmonary Vasoconstriction by Targeting a Voltage‐Gated K+ Channel in Arterial Smooth Muscle Cells
Authors: Li Shan‐Shan , Ran Ya‐Juan , Zhang Dan‐Dan , Li Shu‐Zhen , Zhu Daling 
Published: January 2014

ABSTRACTPulmonary arterial hypertension (PAH) is associated with sustained vasoconstriction, profound structural remodeling of vasculatures and alterations in Ca2+ homeostasis in arterial smooth muscle cells (SMCs), while the underlying mechanisms are still elusive. By regulating the expression of proteins, microRNAs (miRNAs) are known to play an important role in cell fates including differentiation, apoptosis and proliferation, and may be involved in the development of PAH. Based on our previous study, hypoxia produced a significant increase of the miR‐190 level in the pulmonary artery (PA), here, we used synthetic miR‐190 to mimic the increase in hypoxic conditions and showed evidence for the effects of miR‐190 on pulmonary arterial vasoconstriction and Ca2+ influx in arterial SMCs. Synthetic miR‐190 remarkably enhanced the vasoconstriction responses to phenylephrine (PE) and KCl. The voltage‐gated K+ channel subfamily member, Kcnq5, mRNA was shown to be a target for miR‐190. Meanwhile, miR‐190 antisense oligos can partially reverse the effects of miR‐190 on PASMCs and PAs. Therefore, these results suggest that miR‐190 appears to be a positive regulator of Ca2+ influx, and plays an important role in hypoxic pulmonary vascular constriction. J. Cell. Biochem. 115: 1196–1205, 2014. © 2013 Wiley Periodicals, Inc.
Vinculin Regulates Assembly of Talin: β3 Integrin Complexes
Authors: Nanda Suman Yadav , Hoang Thuy , Patel Priya , Zhang Hao 
Published: January 2014

AbstractVinculin is a talin‐binding protein that promotes integrin‐mediated cell adhesion, but the mechanisms are not understood. Because talin is a direct activator of integrins, we asked whether and how vinculin regulates the formation of integrin: talin complexes. We report that VD1 (aa 1‐258) and its talin‐binding mutant, VD1A50I, bind directly and equally to several β integrin cytoplasmic tails (βCT). Results from competition assays show that VD1, but not VD1A50I, inhibits the interaction of talin (Tn) and talin rod (TnR), but not talin head (TnH) with β3CT. The inhibition observed could be the result of VD1 binding to one or more of the 11 vinculin binding sites (VBSs) in the TnR domain. Our studies demonstrate that VD1 binding to amino acids 482‐911, a VBS rich region, in TnR perturbs the interaction of rod with β3CT. The integrin activation assays done using CHOA5 cells show that activated vinculin enhances αIIbβ3 integrin activation and that the effect is dependent on talin. The TnR domain however shows no integrin activation unlike TnH that shows enhanced integrin activation. The overall results indicate that activated vinculin promotes talin‐mediated integrin activation by binding to accessible VBSs in TnR and thus displacing the TnR from the β3 subunit. The study presented, defines a novel direct interaction of VD1 with β3CT and provides an attractive explanation for vinculin's ability to potentiate integrin‐mediated cell adhesion through directly binding to both TnR and the integrin cytoplasmic tail. J. Cell. Biochem. 115: 1206–1216, 2014. © 2013 Wiley Periodicals, Inc.
Enzymatic and Regulatory Attributes of Trehalose‐6‐Phosphate Phosphatase from Candida utilis and its Role During Thermal Stress
Authors: Lahiri Sagar , Banerjee Shakri , Dutta Trina , Sengupta Shinjinee , Dey Sandip , Roy Rusha , Sengupta Devlina , Chattopadhyay Krishnananda , Ghosh Anil K. 
Published: January 2014

AbstractTrehalose‐6‐phosphate phosphatase (TPP) catalyzes the final step in the biosynthesis of the anti‐stress sugar trehalose. An 82 kDa TPP enzyme was isolated from Candida utilis with 61% yield and 43‐fold purification. The protein sequence, determined by N‐terminal sequencing and MALDI‐TOF analysis, showed significant homology with known TPP sequences from related organisms. The full length gene sequence of TPP of C. utilis was identified using rapid amplification of cDNA ends‐PCR reaction (RACE‐PCR). The gene was cloned and expressed in Escherichia coli BL21. Recombinant TPP enzyme was isolated using affinity chromatography. CD spectroscopy and steady‐state fluorescence revealed that the structural and conformational aspects were identical in both native and recombinant forms. The biochemical properties of the two forms were also similar. Km was determined to be ~0.8 mM. Optimum temperature and pH were found to be 30 °C and 8.5, respectively. Activity was dependent on the presence of divalent cations and inhibited by metal chelators. Methylation‐mediated regulation of TPP enzyme and its effect on the overall survival of the organism under stress were investigated. The results indicated that enhancement of TPP activity by methylation at the Cysteine residues increased resistance of Candida cells against thermal stress. This work involves extensive investigations toward understanding the physico‐chemical properties of the first TPP enzyme from any yeast strain. The mechanism by which methylation regulates its activity has also been studied. A correlation between regulation of trehalose synthesis and survivability of the organism under thermal stress was established. J. Cell. Physiol. 229: 1245–1255, 2014. © 2014 Wiley Periodicals, Inc.
Does MRI Breast “Density” (Degree of Background Enhancement) Correlate With Mammographic Breast Density?
Authors: Hansen Nienke L. , Kuhl Christiane K. , Barabasch Alexandra , Strobel Kevin , Schrading Simone 
Published: January 2014

PurposeTo investigate whether mammographic breast densities and the respective degree of MRI background enhancement would correlate. Mammographic breast density is coded to communicate how likely a cancer is obscured by parenchyma. Similarly, background enhancement in breast MRI could obscure enhancing cancer tissue.Materials and MethodsA total of 468 women underwent standard full‐field digital mammography and dynamic contrast‐enhanced breast MRI in our institution. Mammographic breast density was scored according to the American College of Radiology‐classification; background enhancement in MRI was scored on a 4‐point scale from absent to severe. Breast “density classes” were retrospectively compared by analyzing the differences between scores for mammography and MRI. Statistical correlation was calculated using the Spearman coefficient.ResultsScores matched in 19% of women (90/468) but differed in 81% (378/468). A deviation by 1 point was observed in 33% (157/468), by 2 points in 38% (179/468), by 3 in 9% (42/468). Scores for background enhancement were lower than mammographic scores in 371/468 (79.3%), equivalent in 90/468 (19.2%), and higher in 7/468 (1.5%).ConclusionMammographic breast density does not correlate with the degree of background enhancement in MRI. In the majority of women, scores for background enhancement in MRI will be lower than the respective mammographic density scores. J. Magn. Reson. Imaging 2014;40:483–489. © 2014 Wiley Periodicals, Inc.
Towards new response criteria in neuroendocrine tumors: Which changes in MRI parameters are associated with longer progression‐free survival after radioembolization of liver metastases?
Authors: Ceelen Felix , Theisen Daniel , de Albéniz Xabier García , Auernhammer Christoph J. , Haug Alexander R. , D'Anastasi Melvin , Paprottka Philipp M. , Rist Carsten , Reiser Maximilian F. , Sommer Wieland H. 
Published: January 2014

PurposeTo evaluate the association of therapy‐related changes in imaging parameters with progression‐free survival (PFS) of patients with unresectable liver metastases from neuroendocrine tumors (NETLMs).Materials and MethodsForty‐five radioembolized patients (median age: 62 years; range: 43–75) received a pre‐ and 3 months posttherapeutic magnetic resonance imaging (MRI) examination. The latter were evaluated for tumor size, arterial enhancement, and necrosis pattern. Influences of therapy‐related changes on PFS were analyzed. Statistical analysis included Student's t‐test, Wilcoxon test, Cox regression analysis, and Kaplan–Meier curves.ResultsThe median percentage decrease in sum of diameters was 9.7% (range: 43.9% decrease to 15.4% increase). Twenty‐one patients (47%) showed increased necrosis. Three parameters were associated with significantly longer PFS: a decrease of diameter (hazard ratio [HR]: 0.206; 95% confidence interval [CI]: 0.058–0.725; P = 0.0139), a decrease in tumor arterial enhancement (HR: 0.143; 95% CI: 0.029–0.696; P = 0.0160), and an increase in necrosis after 3 months (HR: 0.321; 95% CI: 0.104–0.990; P = 0.0480). Multivariate analysis revealed that changes in diameter and arterial enhancement have complementary information and are associated independently with long PFS.ConclusionA decrease both in sum of diameters and arterial enhancement of metastases, as well as an increase in necrosis, are associated with significantly longer PFS after radioembolization. J. Magn. Reson. Imaging 2015;41:361–368.© 2013 Wiley Periodicals, Inc.