bluuebunny/crossref_metadata_2025_split huggingface dataset:
Starting continuous improvement; creating a common understanding of stroke care delivery in a general hospital
Authors: Fjermeros Are , Berg Geir Vegard , Holtskog Halvor , Benders Jos 
Published: August 2024

Abstract
Background
Continuous improvement is based on fostering practitioners’ suggestions to modify their own work processes This improvement strategy is widely applied in healthcare but difficult to maintain. The cross-disciplinary nature of many care processes constitutes an extra impediment.

Methods
The study had an explorative design with a qualitative single-case approach. The case presents a project to improve the treatment of patients with thrombotic stroke. Data was obtained via hands on involvement, documents, observations, and interviews with participants in a cross-functional improvement group. A thematic analysis method was employed.

Results
Through learning how tasks were carried out in other disciplines, the participants developed a common understanding of why it took so long to provide treatment to stroke patients. These insights were used to implement practical changes, leading to immediate improvements in stroke care delivery. The results were fed back so that successes became visible. Participants’ understandings of the local context enabled them to convince peers of the rationale of changes, setting in motion a permanent improvement structure. The participants considered that mapping and then assessing the entire workflow across disciplines were relevant methods for improving the quality of patient care.

Conclusion
Starting an improvement project in a cross disciplinary environment requires deep engagement on the part of professionals. A quintessential prerequisite is therefore the realization that the quality of care depends on cross-disciplinary cooperation. A facilitated learning arena needs to (1) create insights into each other’s colleagues’ tasks and process interdependencies, (2) increase understanding of how the distribution of tasks among specialist units affects the quality of care, and (3) frequently report and provide feedback on results to keep the process going.
Enhanced anti-tumor effects by combination of tucatinib and radiation in HER2-overexpressing human cancer cell lines
Authors: Amrell Lukas , Bär Eric , Glasow Annegret , Kortmann Rolf-Dieter , Seidel Clemens , Patties Ina 
Published: August 2024

Abstract
Background
Tucatinib (TUC), a HER2-directed tyrosine kinase inhibitor, is the first targeted drug demonstrating intracranial efficacy and significantly prolonged survival in metastatic HER2-positive breast cancer (BC) patients with brain metastases. Current treatments for brain metastases often include radiotherapy, but little is known about the effects of combination treatment with TUC. Therefore, we examined the combined effects of irradiation and TUC in human HER2-overexpressing BC, non-small cell lung cancer (NSCLC), and colorectal cancer (CRC) cell lines. For the latter two, a standard therapy successfully targeting HER2 is yet to be established.

Methods
Nine HER2-overexpressing (BC: BT474, ZR7530, HCC1954; CRC: LS411N, DLD1, COLO201; NSCLC: DV90, NCI-H1781) and three control cell lines (BC: MCF7, HCC38; NSCLC: NCI-H2030) were examined. WST-1 assay (metabolic activity), BrdU ELISA (proliferation), γH2AX assay (DNA double-strand breaks (DSB), Annexin V assay (apoptosis), and clonogenic assay (clonogenicity) were performed after treatment with TUC and/or irradiation (IR). The relevance of the treatment sequence was analyzed exemplarily.

Results
In BC, combinatorial treatment with TUC and IR significantly decreased metabolic activity, cell proliferation, clonogenicity and enhanced apoptotis compared to IR alone, whereby cell line-specific differences occurred. In the PI3KCA-mutated HCC1954 cell line, addition of alpelisib (ALP) further decreased clonogenicity. TUC delayed the repair of IR-induced DNA damage but did not induce DSB itself. Investigation of treatment sequence indicated a benefit of IR before TUC versus IR after TUC. Also in CRC and NSCLC, the combination led to a stronger inhibition of metabolic activity, proliferation, and clonogenic survival (only in NSCLC) than IR alone, whereby about 10-fold higher concentrations of TUC had to be applied than in BC to induce significant changes.

Conclusion
Our data indicate that combination of TUC and IR could be more effective than single treatment strategies for BC. Thereby, treatment sequence seems to be an important factor. The lower sensitivity to TUC in NSCLC and particularly in CRC (compared to BC) implicates, that tumor promotion there might be less HER2-related. Combination with inhibitors of other driver mutations may aid in overcoming partial TUC resistance. These findings are of high relevance to improve long-time prognosis especially in brain-metastasized situations given the intracranial activity of TUC.
Knowledge, attitude, and perception about Marburg virus in healthcare workers of India
Authors: Mehta Vini , Negi Sapna , Mathur Ankita , Obulareddy Vishnu Teja , Shaik Riyaz Ahamed , Ahmed Mohammed Shakil , Miraj Mohammed 
Published: August 2024

Abstract
Background
The recent Marburg virus outbreaks in Equatorial Guinea and Tanzania in March 2023 have sparked fears about potential worldwide spread. Thus, assessing healthcare workers’ (HCW) knowledge of the disease is critical, particularly in India, where the healthcare system is underfunded and understaffed and already has an immense burden of infectious illnesses.

Methods
We carried out a cross-sectional web-based survey among 300 healthcare workers including doctors, nurses, and pharmacists. The Google survey form was circulated online via email. To recruit participants, a convenience sample technique was utilized. Descriptive and logistic regression was applied to analyse data.

Results
50.9% were aware of Marburg virus disease, and 60.5% had a favourable attitude towards Marburg virus disease (MARVD) control and prevention. Bleeding was cited as the most common clinical symptom of MARVD by 53.3% of participants. Regarding the mode of transmission, 51.6% were aware of how MARVD is spread. Almost all HCWs (99%) claimed they would accept an approved vaccine for themselves, however, the readiness to receive experimental treatments was less certain. The logistic regression model showed gender [female—OR: 2.8 (1.75 to 3.5), p = 0.001] and job roles [pharmacist—OR: 4.1 (2.9 to 7.2), P < 0.001] as predictors of poor KAP about MARVD.

Conclusion
Although there was a high degree of awareness about MARVD, there were notable knowledge gaps and differences in attitudes among healthcare professionals. This underlines the importance of customized training programs for each group to increase healthcare workers’ knowledge of MARVD.
An uncommon encounter: crossed fused renal ectopia with singular ureter: a case report
Authors: Dahal Prajwal , Dawadi Kapil , Tamang Ongden Yonjen , Parajuli Sabina , Dhakal Natasha 
Published: August 2024

Abstract
Background
Crossed fused renal ectopia (CFRE) is a common congenital anomaly where one kidney is positioned abnormally on the opposite side of the midline, often fused with the other kidney. However, single ureter draining crossed fused renal ectopia is a rare occurrence.

Case report
Here, we report a case of crossed fused renal ectopia with a single ureter in a 46-year-old Nepali male who presented with history of lithuria. Computed tomography revealed that the left kidney was situated on the right side and fused with the right kidney. The renal pelvises of both kidneys were fused, and a single ureter, located on the right side, was draining both kidneys into the bladder. The patient was advised to have regular follow-ups.

Conclusion
Crossed fused renal ectopia with a single ureter represents a rare renal anomaly. Asymptomatic patients can typically be managed conservatively. Regular follow-up is recommended to monitor renal function, calculus formation, infections, and malignant changes.
From follicle to blastocyst: microRNA-34c from follicular fluid-derived extracellular vesicles modulates blastocyst quality
Authors: Benedetti Camilla , Pavani Krishna Chaitanya , Gansemans Yannick , Azari-Dolatabad Nima , Pascottini Osvaldo Bogado , Peelman Luc , Six Rani , Fan Yuan , Guan Xuefeng , Deserranno Koen , Fernández-Montoro Andrea , Hamacher Joachim , Van Nieuwerburgh Filip , Fair Trudee , Hendrix An , Smits Katrien , Van Soom Ann 
Published: August 2024

Abstract
Background
Within the follicular fluid, extracellular vesicles (EVs) guide oocyte growth through their cargo microRNAs (miRNAs). Here, we investigated the role of EVs and their cargo miRNAs by linking the miRNAs found in EVs, derived from the fluid of an individual follicle, to the ability of its oocyte to become a blastocyst (competent) or not (non-competent).

Methods
Bovine antral follicles were dissected, categorized as small (2–4 mm) or large (5–8 mm) and the corresponding oocytes were subjected to individual maturation, fertilization and embryo culture to the blastocyst stage. Follicular fluid was pooled in 4 groups (4 replicates) based on follicle size and competence of the corresponding oocyte to produce a blastocyst. Follicular fluid-derived EVs were isolated, characterized, and subjected to miRNA-sequencing (Illumina Miseq) to assess differential expression (DE) in the 4 groups. Functional validation of the effect of miR-34c on embryo development was performed by supplementation of mimics and inhibitors during in vitro maturation (IVM).

Results
We identified 16 DE miRNAs linked to oocyte competence when follicular size was not considered. Within the large and small follicles, 46 DE miRNAs were driving blastocyst formation in each group. Comparison of EVs from competent small and large follicles revealed 90 DE miRNAs. Cell regulation, cell differentiation, cell cycle, and metabolic process regulation were the most enriched pathways targeted by the DE miRNAs from competent oocytes. We identified bta-miR-34c as the most abundant in follicular fluid containing competent oocytes. Supplementation of miR-34c mimic and inhibitor during IVM did not affect embryo development. However, blastocyst quality, as evidenced by higher cell numbers, was significantly improved following oocyte IVM in the presence of miR-34c mimics, while miR-34c inhibitors resulted in the opposite effect.

Conclusion
This study demonstrates the regulatory effect of miRNAs from follicular fluid-derived EVs on oocyte competence acquisition, providing a further basis for understanding the significance of miRNAs in oocyte maturation and embryonic development. Up-regulation of miR-34c in EVs from follicular fluid containing competent oocytes and the positive impact of miR-34c mimics added during IVM on the resulting blastocysts indicate its pivotal role in oocyte competence.
Astragalus polysaccharides-induced gut microbiota play a predominant role in enhancing of intestinal barrier function of broiler chickens
Authors: Yang Jiantao , Sun Yanpeng , Wang Qianggang , Yu Shanglin , Li Yanhe , Yao Bin , Yang Xiaojun 
Published: August 2024

Abstract
Background
The intestinal barrier is the first line of defense against intestinal invasion by pathogens and foreign antigens and is closely associated with the gut microbiota. Astragalus polysaccharides (APS) have a long history of use in traditional Chinese medicine owing to its protective properties against intestinal barrier function. The mechanism of APS-induced gut microbiota enhancing intestinal barrier function is urgently needed.

Results
Dietary polysaccharide deprivation induced intestinal barrier dysfunction, decreased growth performance, altered microbial composition (Faecalibacterium, Dorea, and Coprobacillus), and reduced isobutyrate concentration. The results showed that APS facilitates intestinal barrier function in broiler chickens, including a thicker mucus layer, reduced crypt depth, and the growth of tight junction proteins. We studied the landscape of APS-induced gut microbiota and found that APS selectively promoted the growth of Parabacteroides, a commensal bacterium that plays a predominant role in enhancing intestinal barrier function. An in vitro growth assay further verified that APS selectively increased the abundance of Parabacteroides distasonis and Bacteroides uniformis. Dietary APS supplementation increased the concentrations of isobutyrate and bile acid (mainly chenodeoxycholic acid and deoxycholate acid) and activated signaling pathways related to intestinal barrier function (such as protein processing in the endoplasmic reticulum, tight junctions, and adherens junction signaling pathways).

Conclusions
APS intervention restored the dietary polysaccharide-induced dysfunction of the intestinal barrier by selectively promoting the abundance of Parabacteroides distasonis, and increasing the concentrations of isobutyrate and bile acids (mainly CDCA and DCA). These findings suggest that APS-induced gut microbiota and metabolic niches are promising strategies for enhancing intestinal barrier function.

Graphical Abstract
The prospect of ‘interstitial practice’ in the in-between spaces of Caracas
Authors: García Alcaraz Teresa 
Published: August 2024

AbstractThis paper delves into interstitial practice, a phenomenon unfolding in the in-between spaces, with a specific focus on Caracas, Venezuela. It explores the complex relationship between space, art, and power, comprehensively analysing interstitial practice as a flexible resource shaped by diverse interests, thus empowering its political nature and impact on immediate surroundings. Through a multi-faced methodological approach, findings suggest that the institutionalisation of this practice near vulnerable areas becomes a focal point for political sources, emphasising the community sentiment to shape a collective urban identity. This research offers a foundational reference for understanding the dynamic interplay of territory, urban interventions, and power in urban contexts and provides profound insights into the transformative potential of in-between spaces in contemporary cities.
The effect of traditional processing craft on the hygroscopicity of palm leaf manuscripts
Authors: Yu Dehao , Li Xuanhua , Sun Si’ao , Guo Hong , Luo Hao , Zhu Jinmeng , Li Liusan , Wang Shan , Han Liuyang 
Published: August 2024

Abstract
Palm leaf manuscripts, which are crucial carriers of historical, religious, scientific, and artistic information in East and Southeast Asia, specifically encapsulate significant aspects of Buddhist culture and thus require comprehensive research and preservation efforts. The base material of palm leaf manuscripts is processed palm leaves, which are hygroscopic and profoundly affected by environmental humidity. Currently, there is a research gap regarding the impact of traditional processing crafts and natural aging on the hygroscopicity of palm leaf manuscripts. Utilizing dynamic water vapor sorption (DVS), the hygroscopic properties of palm leaves from various years were assessed before and after traditional processing in Yunnan Province, China. The results show that traditional processing slightly increases the equilibrium moisture content (EMC) in environments with 0 to 60% relative humidity (RH), but significantly lowers EMC in high humidity environments, with reductions up to 19.01%. Additionally, hysteresis doubled post-processing, indicating enhanced stability under fluctuating humidity conditions. Sorption models suggest that traditional processing increases the number of adsorption sites while reducing physical adsorption or capillary condensation. FT-IR (Fourier-transform infrared spectroscopy) analysis indicates that the relative contents of cellulose and hemicellulose were reduced by 39.90% and 3.97%, respectively. Degradation occurring in both the crystalline and amorphous regions of cellulose. After natural aging, the hygroscopicity of processed palm leaves improved across the entire humidity range of 0 to 95%, and there was a slight increase in hysteresis. This is due to the increase in both adsorption sites and physical adsorption capabilities. FT-IR results also indicate that the relative contents of cellulose and hemicellulose were decreased by 57.52% and 19.83% after nature aging, respectively. These findings confirm that traditional processing improves the writability and humidity resilience of the leaves, while natural aging enhances their overall hygroscopic properties. This research contributes to our understanding of how humidity damages palm leaf manuscripts. aids in determining optimal RH ranges for storage, and assesses the effectiveness of consolidation treatments in their long–term preservation.
Advancing cybersecurity: a comprehensive review of AI-driven detection techniques
Authors: Salem Aya H. , Azzam Safaa M. , Emam O. E. , Abohany Amr A. 
Published: August 2024

AbstractAs the number and cleverness of cyber-attacks keep increasing rapidly, it's more important than ever to have good ways to detect and prevent them. Recognizing cyber threats quickly and accurately is crucial because they can cause severe damage to individuals and businesses. This paper takes a close look at how we can use artificial intelligence (AI), including machine learning (ML) and deep learning (DL), alongside metaheuristic algorithms to detect cyber-attacks better. We've thoroughly examined over sixty recent studies to measure how effective these AI tools are at identifying and fighting a wide range of cyber threats. Our research includes a diverse array of cyberattacks such as malware attacks, network intrusions, spam, and others, showing that ML and DL methods, together with metaheuristic algorithms, significantly improve how well we can find and respond to cyber threats. We compare these AI methods to find out what they're good at and where they could improve, especially as we face new and changing cyber-attacks. This paper presents a straightforward framework for assessing AI Methods in cyber threat detection. Given the increasing complexity of cyber threats, enhancing AI methods and regularly ensuring strong protection is critical. We evaluate the effectiveness and the limitations of current ML and DL proposed models, in addition to the metaheuristic algorithms. Recognizing these limitations is vital for guiding future enhancements. We're pushing for smart and flexible solutions that can adapt to new challenges. The findings from our research suggest that the future of protecting against cyber-attacks will rely on continuously updating AI methods to stay ahead of hackers' latest tricks.
Interpolation-split: a data-centric deep learning approach with big interpolated data to boost airway segmentation performance
Authors: Cheung Wing Keung , Pakzad Ashkan , Mogulkoc Nesrin , Needleman Sarah Helen , Rangelov Bojidar , Gudmundsson Eyjolfur , Zhao An , Abbas Mariam , McLaverty Davina , Asimakopoulos Dimitrios , Chapman Robert , Savas Recep , Janes Sam M. , Hu Yipeng , Alexander Daniel C. , Hurst John R. , Jacob Joseph 
Published: August 2024

AbstractThe morphology and distribution of airway tree abnormalities enable diagnosis and disease characterisation across a variety of chronic respiratory conditions. In this regard, airway segmentation plays a critical role in the production of the outline of the entire airway tree to enable estimation of disease extent and severity. Furthermore, the segmentation of a complete airway tree is challenging as the intensity, scale/size and shape of airway segments and their walls change across generations. The existing classical techniques either provide an undersegmented or oversegmented airway tree, and manual intervention is required for optimal airway tree segmentation. The recent development of deep learning methods provides a fully automatic way of segmenting airway trees; however, these methods usually require high GPU memory usage and are difficult to implement in low computational resource environments. Therefore, in this study, we propose a data-centric deep learning technique with big interpolated data, Interpolation-Split, to boost the segmentation performance of the airway tree. The proposed technique utilises interpolation and image split to improve data usefulness and quality. Then, an ensemble learning strategy is implemented to aggregate the segmented airway segments at different scales. In terms of average segmentation performance (dice similarity coefficient, DSC), our method (A) achieves 90.55%, 89.52%, and 85.80%; (B) outperforms the baseline models by 2.89%, 3.86%, and 3.87% on average; and (C) produces maximum segmentation performance gain by 14.11%, 9.28%, and 12.70% for individual cases when (1) nnU-Net with instant normalisation and leaky ReLU; (2) nnU-Net with batch normalisation and ReLU; and (3) modified dilated U-Net are used respectively. Our proposed method outperformed the state-of-the-art airway segmentation approaches. Furthermore, our proposed technique has low RAM and GPU memory usage, and it is GPU memory-efficient and highly flexible, enabling it to be deployed on any 2D deep learning model.
Initiation of a novel text messaging system in total knee and hip arthroplasty
Authors: van der Merwe Johannes M. , Nickol Michaela E. 
Published: August 2024

Abstract
Background
The primary objective of this study was to investigate whether using a novel text messaging system improves patient overall satisfaction compared to standard care. Secondary objectives included assessing the impact of the text messaging system on decreasing narcotic usage, the number of emergency department visits, the range of flexion and extension, and number of telephone calls to the surgeon’s office.

Methods
We enrolled 217 patients to either receive informative text messages (text messaging group, n = 86) or no additional text messages (conventional group, n = 131). Patients self-reported results on a questionnaire at the 6-week follow-up regarding the primary and secondary objectives. The active range of motion of total knee arthroplasty patients was recorded by the surgeon or treating physiotherapist.

Results
There was no significant difference in overall satisfaction (P = 0.644), narcotic cessation (P = 0.185), range of motion (Flexion P = 0.521; Extension P = 0.515), and emergency department visits (P = 0.650) between the two groups. There was a statistically significant decrease in surgeon office calls favoring the text messaging group (P = 0.029). A subgroup analysis revealed that the statistical difference was mainly in the TKA group (P = 0.046).

Conclusions
A novel text messaging system may help reduce the work burden by decreasing telephone calls to the surgeon’s office. While satisfaction, narcotic usage, emergency department visits, and range of motion did not significantly differ, patients endorsed the system for friends/family.