bluuebunny/crossref_metadata_2025_split huggingface dataset:
What Wyatt really did to Aretino's Sette Salmi
Authors: Rossiter William T. 
Published: July 2015

AbstractThis article interrogates the critical tradition which claims that Wyatt's Penitential Psalms speak with the authentic personal voice of early English Protestantism. The title references C. S. Lewis's famous essay, ‘What Chaucer Really Did to Il Filostrato', in which Chaucer is seen to ‘medievalize’ Boccaccio's ‘Renaissance’ poem, in order to question the assumption that Wyatt ‘Protestantizes' his primary Italian (Catholic) source, Pietro Aretino's I sette salmi de la penitentia di David (1534). This critical tradition, it is argued, is itself predicated upon the same periodization underpinning Lewis's essay, whereby the erroneous binary opposition Catholic/Protestant becomes metonymic of medieval/Renaissance. Applying this clear‐cut confessional division to the late 1530s, when Wyatt wrote (or at the very least began) his sequence, is an anachronism. What this article argues, rather, is that Wyatt's reading and rewriting of Aretino's paraphrase and Antonio Brucioli's commentary on the Penitential Psalms — which also, I argue, influenced Aretino — helps him to reform certain of the equivocations and contradictions found in the Ten Articles (1536) and the Bishops' Book (1537) into a more consistent poetics of grace; notwithstanding his diverse other sources.
Psychological effects of (non)employment: A cross‐national comparison of the United States and Japan
Authors: Gnambs Timo , Stiglbauer Barbara , Selenko Eva 
Published: July 2015

The involuntary loss of employment has been shown to deteriorate subjective well‐being. Adopting a cross‐cultural perspective on Jahoda's () deprivation model this study examines several latent and manifest benefits of work that were expected to mediate the effects of employment status on well‐being. It was hypothesized that in more collectivistic societies the decline in subjective well‐being would be a consequence of a diminished sense of collective purpose for the non‐employed, whereas in individualistic societies the crucial factors would be a loss of social status and financial benefits. The findings from two representative national surveys conducted in the United States (N = 1,093) and Japan (N = 647) provided partial support for these hypotheses. Cultural differences moderated the effects of employment status on the benefits of work. As a consequence, different processes mediated the decline in well‐being for the non‐employed in the two countries. These results are embedded within the wider discourse on culture and its effect on unemployment.
Stigma, Attitudes, and Help‐Seeking Intentions for Psychological Problems in Relation to Regional Suicide Rates
Authors: Reynders Alexandre , Kerkhof Ad J. F. M. , Molenberghs Geert , Van Audenhove Chantal 
Published: July 2015

In this ecological study, we investigated whether help‐seeking related to stigma, intentions, and attitudes toward suicide are associated with the suicide rates of 20 regions within the Netherlands and Belgium. Significant associations were found between regional suicide rates and the intention to seek informal help (β = −1.47, p = .001), self‐stigma (β = 1.33, p = .038), and shame (β = .71, p = .030). The association between self‐stigma and suicide rate was mediated by intentions to seek informal help. These results suggest that to promote suicide prevention at the level of the regional population, stigma, shame, and intentions to seek help should be targeted in the public domain.
Allogenic benefit in stem cell therapy: cardiac repair and regeneration
Authors: Al‐Daccak R. , Charron D. 
Published: July 2015

AbstractStem cell (SC)‐based therapies are a developing mean to repair, restore, maintain, or enhance organ functioning through life span. They are in particular a fast track to restore function in failing heart. Various types of SCs have been used in experimental and clinical studies showing the potential of these cells to revolutionize the treatment of heart diseases. Autologous cells have been privileged to overpass immunological barriers. The field has progressed tremendously and the hurdles, which have been largely overlooked in the excitement over the expected benefit the immunogenicity, have been revealed. Also, manufacturing of patient‐specific clinical grade SC product, whether adult stem or reprogrammed induced pluripotent SCs, and the availability of these cells in sufficient amounts and status when needed is questionable. In contrast, adult SCs derived from healthy donors, thus allogeneic, have the advantage to be immediately available as an ‘off‐the‐shelf’ therapeutic product. The challenge is to overcome the immunological barriers to their transplantation. Recent research provided new insights into the mode of action and immune behavior of SCs in autologous as well as allogeneic settings. Lessons are learned and immune paradigms are changing: allogenicity, if balanced could be part of the dynamic and durable mechanisms that are critical to sustain cardiac regeneration and repair. We discuss the hurdles, lessons, and advances accomplished in the field through the progressive journey of cardiac‐derived stem/progenitor cells toward allogeneic cardiac regenerative/reparative therapy.
What do the stats tell us? Engaging elementary children in probabilistic reasoning based on data analysis
Authors: Hourigan Mairéad , Leavy Aisling 
Published: July 2015

SummaryAs part of Japanese Lesson study research focusing on ‘comparing and describing likelihoods’, fifth grade elementary students used real‐world data in decision‐making. Sporting statistics facilitated opportunities for informal inference, where data were used to make and justify predictions.
Heterogeneous Firms, Globalisation and the Distance Puzzle
Authors: Larch Mario , Norbäck Pehr‐Johan , Sirries Steffen , Urban Dieter M. 
Published: July 2015

AbstractDespite the strong pace of globalisation, the distance effect on trade is persistent or even growing over time (Disdier and Head, 2008). To solve this distance puzzle, we use the recently developed gravity equation estimator from Helpman et al. (2008) (HMR henceforth). Using three different data sets, we find that the distance coefficient increases over time when ordinary least squares (OLS) is used, while the non‐linear estimation of HMR leads to a decline in the distance coefficient over time. The distance puzzle, thus, arises from a growing bias of OLS estimates. The latter is explained by an increase in the importance of the bias from omitting the number of heterogeneous exporting firms relative to the bias from omitting zero trade flows. Furthermore, we show that including zero trade flows cannot solve the distance puzzle when using HMR. The HMR estimates are strongly correlated with the time pattern in freight costs reported by Hummels (2007).
Differential regulation of pericyte function by the CXC receptor 3
Authors: Bodnar Richard J. , Wells Alan 
Published: July 2015

ABSTRACTPericytes are mural cell that have been found to play important roles in promoting blood vessel development and regulating blood flow. The signals that attract pericytes to maturing vessels during the resolution phase of wound healing are unknown. In this study, we examine the role of the chemokine receptor CXC receptor 3 (CXCR3) ligands, as they are produced by maturing endothelial cells. Pericytes isolated from muscle and retina were found to by and large only express the B‐isoform of CXCR3 (CXCR3B), with expression being independent of the mitotic state of the cells. Pericyte stimulation with the CXCR3 ligands Mig (CXCL9), IP‐9/I‐TAC (CXCL11), or IP‐10 (CXCL10) resulted in the activation of ERK but not AKT. Treatment with Mig or IP‐9, but not IP‐10, enhanced p38MAPK phosphorylation. Interestingly, while cyclic adenosine monophosphate is generated downstream of CXCR3B in other cells, protein kinase A activation was not observed in these pericytes when treated with these three CXCR3 ligands. The increase in ERK activity resulted in a slight increase in cell transmigration, with the inhibition of ERK leading to a decrease in CXCR3B mediated migration and inhibition of p38MAPK reducing transmigration through small pores. These ligands did not affect proliferation. These data are the first to characterize CXCR3B as the predominant isoform expressed on pericytes, and was found on these diverse cells isolated from both muscle and eye. We also show that CXCR3B signaling stimulates transmigration of barrier pores in pericytes as opposed to its inhibitory affects on endothelial cells and fibroblasts. These findings characterize a novel role for the CXCR3B in regulating cellular function. Taken together these data show a role for CXCR3B in regulating pericyte function.
“Are Your Clients Having Fun?” The Implications of Respondents’ Preferences for the Delivery of Group Exercise Programs for Falls Prevention
Authors: McPhate Lucy , Simek Emily M. , Haines Terry P. , Hill Keith D. , Finch Caroline F. , Day Lesley 
Published: July 2015

Background:Group exercise has been shown to be effective in preventing falls; however, adherence to these interventions is often poor. Older adults’ preferences for how these programs can be delivered are unknown.Objective:To identify older people’s preferences for how group exercise programs for falls prevention can be delivered.Design:A two-wave, cross-sectional, state-wide telephone survey was undertaken. Respondents were community-dwelling men and women aged 70+ in Victoria, Australia.Methods:Open-ended questions were asked to elicit information regarding respondent preferences of the program, which were analyzed using a framework approach.Results:Ninetyseven respondents completed the follow-up survey. The results indicate that older adults most frequently report the short-term advantages and disadvantages when describing their preferences for group exercise, such as enjoyment, social interaction, and leader qualities. Longer-term advantages such as falls prevention were described less frequently.Conclusions:This study indicates the importance of interpersonal skills, and that the opportunity for social interaction should not be overlooked as a positive feature of a group exercise program.
Draft Genome Sequence of Staphylococcus sciuri subsp.
sciuri
Strain Z8, Isolated from Human Skin
Authors: Hu Xinjun , Zheng Beiwen , Jiang Haiyin , Kang Yi , Cao Qing , Ning Huibin , Shang Jia 
Published: July 2015

ABSTRACT

Staphylococcus sciuri
subsp.
sciuri
strain Z8 was isolated from a skin wound infection of a patient with infective endocarditis. To the best of our knowledge, the genome sequence of the species
S. sciuri
has not been previously studied. The complete genome sequence of strain Z8 includes a genome of 2,620,868 bp (32.43% GC content) without any plasmids.
Complete Genome Sequence of a Field Isolate of Classical Swine Fever Virus Belonging to Subgenotype 2.1b from Hunan Province, China
Authors: Shao Weixing , Liu Shuang , Wu Faxing , Zhang Zhi , Dong Yaqin , Li Xiaocheng 
Published: July 2015

ABSTRACT
We report the complete genome sequence of a field isolate of classical swine fever virus (CSFV), Hunan 23/2013, belonging to the predominant subgenotype 2.1b. This strain was originally isolated from diseased pigs in Hunan Province, China. This report will help in understanding the molecular diversity of CSFV stains circulating in China and in selecting and developing a suitable vaccine candidate for CSF control.
Draft Genome Sequence of the Ale-Fermenting Saccharomyces cerevisiae Strain GSY2239
Authors: U’Ren Jana M. , Wisecaver Jennifer H. , Paek Andrew L. , Dunn Barbara L. , Hurwitz Bonnie L. 
Published: July 2015

ABSTRACT

Saccharomyces cerevisiae
strain GSY2239 is derived from an industrial yeast strain used to ferment ale-style beer. We present here the 11.5-Mb draft genome sequence for this organism.
Draft Genome Sequence of Thermophilic
Exiguobacterium
sp. Strain JLM-2, Isolated from Deep-Sea Ferromanganese Nodules
Authors: Zhang De-Chao , Liu Yan-Xia , Huo Ying-Yi , Xu Xue-Wei , Li Xin-Zheng 
Published: July 2015

ABSTRACT

Exiguobacterium
sp. strain JLM-2 is a thermophilic bacterium isolated from deep-sea ferromanganese (FeMn) nodules. The estimated genome of this strain is 2.9 Mb, with a G+C content of 48.32%. It has a novel circular 15,570-bp plasmid. The draft genome sequence may provide useful information about Mn-microbe interactions and the genetic basis for tolerance to environment stresses.
Draft Genome Sequence of Broad-Spectrum Antifungal Bacterium Burkholderia gladioli Strain NGJ1, Isolated from Healthy Rice Seeds
Authors: Jha Gopaljee , Tyagi Isha , Kumar Rajeev , Ghosh Srayan 
Published: July 2015

ABSTRACT

We report here the draft genome sequence of
Burkholderia gladioli
strain NGJ1. The strain was isolated from healthy rice seeds and exhibits broad-spectrum antifungal activity against several agriculturally important pathogens, including
Rhizoctonia solani
,
Magnaporthe oryzae
,
Venturia inaequalis
, and
Fusarium oxysporum
.
Complete Genome Sequence of an
Edwardsiella piscicida
-Like Species Isolated from Diseased Grouper in Israel
Authors: Reichley Stephen R. , Waldbieser Geoffrey C. , Ucko Michal , Colorni Angelo , Dubytska Lidiya , Thune Ronald L. , Lawrence Mark L. , Griffin Matt J. 
Published: July 2015

ABSTRACT

The
Edwardsiella piscicida
-like sp. is a Gram-negative facultative anaerobe that causes disease in some fish species. We report here the complete genome sequence of a virulent isolate from a diseased white grouper (
Epinephelus aeneus
) raised on the Red Sea in Israel, which contains a chromosome of 3,934,167 bp and no plasmids.
A Single Nucleotide Polymorphism at the TaqMan Probe-Binding Site Impedes Real-Time Reverse Transcription-PCR-Based Detection of Norovirus GII.4 Sydney
Authors: Zhuo Ran , Hasing Maria Eloisa ,   Team of Molecular Diagnostics, Pang Xiaoli 
Published: July 2015

ABSTRACT
A single nucleotide polymorphism (SNP) in the TaqMan probe-binding site resulted in decreased sensitivity of real-time reverse transcription-PCR (RT-rtPCR) for detection of norovirus genogroup II genotype 4 (GII.4) Sydney. A new degenerate probe was designed that improved the sensitivity of the detection while not interfering with the detection of other GII and GI strains.
Systematic Evaluation of Different Nucleic Acid Amplification Assays for Cytomegalovirus Detection: Feasibility of Blood Donor Screening
Authors: Vollmer T. , Knabbe C. , Dreier J. 
Published: July 2015

ABSTRACTAcute primary cytomegalovirus (CMV) infections, which commonly occur asymptomatically among blood donors, represent a significant risk for serious morbidity in immunocompromised patients (a major group of transfusion recipients). We implemented a routine CMV pool screening procedure for plasma for the identification of CMV DNA-positive donors, and we evaluated the sensitivities and performance of different CMV DNA amplification systems. Minipools (MPs) of samples from 18,405 individual donors (54,451 donations) were screened for CMV DNA using the RealStar CMV PCR assay (Altona Diagnostic Technologies), with a minimum detection limit of 11.14 IU/ml. DNA was extracted with a high-volume protocol (4.8 ml, Chemagic Viral 5K kit; PerkinElmer) for blood donor pool screening (MP-nucleic acid testing [NAT]) and with the Nuclisens easyMAG system (0.5 ml; bioMérieux) for individual donation (ID)-NAT. In total, six CMV DNA-positive donors (0.03%) were identified by routine CMV screening, with DNA concentrations ranging from 4.35 × 102to 4.30 × 103IU/ml. Five donors already showed seroconversion and detectable IgA, IgM, and/or IgG antibody titers (IgA+/IgM+/IgG−or IgA+/IgM+/IgG+), and one donor showed no CMV-specific antibodies. Comparison of three commercial assays, i.e., the RealStar CMV PCR kit, the Sentosa SA CMV quantitative PCR kit (Vela Diagnostics), and the CMV R-gene PCR kit (bioMérieux), for MP-NAT and ID-NAT showed comparably good analytical sensitivities, ranging from 10.23 to 11.14 IU/ml (MP-NAT) or from 37.66 to 57.94 IU/ml (ID-NAT). The clinical relevance of transfusion-associated CMV infections requires further investigation, and the evaluated methods present powerful basic tools providing sensitive possibilities for viral testing. The application of CMV MP-NAT facilitated the identification of one donor with a window-phase donation during acute primary CMV infection.
Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
Authors: Brown Rebecca J. , Holden Matthew T. G. , Spiller O. Brad , Chalker Victoria J. 
Published: July 2015

ABSTRACTMycoplasma pneumoniaeis a major human respiratory pathogen causing both upper and lower respiratory disease in humans of all ages, and it can also result in other serious extrapulmonary sequelae. A multilocus sequence typing (MLST) scheme forM. pneumoniaewas developed based on the sequences of eight housekeeping genes (ppa,pgm,gyrB,gmk,glyA,atpA,arcC, andadk) and applied to 55M. pneumoniaeclinical isolates and the two type strains M129 and FH. A total of 12 sequence types (STs) resulted for 57M. pneumoniaeisolates tested, with a discriminatory index of 0.21 STs per isolate. The MLST loci used in this scheme were shown to be stable in 10 strains following 10 sequential subculture passages. Phylogenetic analysis of concatenated sequences of the eight loci indicated two distinct genetic clusters that were directly linked to multilocus variable-number tandem repeat analysis (MLVA) type. Genetic MLST clustering was confirmed by genomic sequence analysis, indicating that the MLST scheme developed in this study is representative of the genome. Furthermore, this MLST scheme was shown to be more discriminatory than both MLVA and P1 typing for theM. pneumoniaeisolates examined, providing a method for further and more detailed analysis of observed epidemic peaks ofM. pneumoniaeinfection. This scheme is supported by a public Web-based database (http://pubmlst.org/mpneumoniae).
Limited Variation in BK Virus T-Cell Epitopes Revealed by Next-Generation Sequencing
Authors: Sahoo Malaya K. , Tan Susanna K. , Chen Sharon F. , Kapusinszky Beatrix , Concepcion Katherine R. , Kjelson Lynn , Mallempati Kalyan , Farina Heidi M. , Fernández-Viña Marcelo , Tyan Dolly , Grimm Paul C. , Anderson Matthew W. , Concepcion Waldo , Pinsky Benjamin A. 
Published: July 2015

ABSTRACT
BK virus (BKV) infection causing end-organ disease remains a formidable challenge to the hematopoietic cell transplant (HCT) and kidney transplant fields. As BKV-specific treatments are limited, immunologic-based therapies may be a promising and novel therapeutic option for transplant recipients with persistent BKV infection. Here, we describe a whole-genome, deep-sequencing methodology and bioinformatics pipeline that identify BKV variants across the genome and at BKV-specific HLA-A2-, HLA-B0702-, and HLA-B08-restricted CD8 T-cell epitopes. BKV whole genomes were amplified using long-range PCR with four inverse primer sets, and fragmentation libraries were sequenced on the Ion Torrent Personal Genome Machine (PGM). An error model and variant-calling algorithm were developed to accurately identify rare variants. A total of 65 samples from 18 pediatric HCT and kidney recipients with quantifiable BKV DNAemia underwent whole-genome sequencing. Limited genetic variation was observed. The median number of amino acid variants identified per sample was 8 (range, 2 to 37; interquartile range, 10), with the majority of variants (77%) detected at a frequency of <5%. When normalized for length, there was no statistical difference in the median number of variants across all genes. Similarly, the predominant virus population within samples harbored T-cell epitopes similar to the reference BKV strain that was matched for the BKV genotype. Despite the conservation of epitopes, low-level variants in T-cell epitopes were detected in 77.7% (14/18) of patients. Understanding epitope variation across the whole genome provides insight into the virus-immune interface and may help guide the development of protocols for novel immunologic-based therapies.
Redundant Function of Plasmacytoid and Conventional Dendritic Cells Is Required To Survive a Natural Virus Infection
Authors: Kaminsky Lauren W. , Sei Janet J. , Parekh Nikhil J. , Davies Michael L. , Reider Irene E. , Krouse Tracy E. , Norbury Christopher C. 
Published: July 2015

ABSTRACTViruses that spread systemically from a peripheral site of infection cause morbidity and mortality in the human population. Innate myeloid cells, including monocytes, macrophages, monocyte-derived dendritic cells (mo-DC), and dendritic cells (DC), respond early during viral infection to control viral replication, reducing virus spread from the peripheral site. Ectromelia virus (ECTV), an orthopoxvirus that naturally infects the mouse, spreads systemically from the peripheral site of infection and results in death of susceptible mice. While phagocytic cells have a requisite role in the response to ECTV, the requirement for individual myeloid cell populations during acute immune responses to peripheral viral infection is unclear. In this study, a variety of myeloid-specific depletion methods were used to dissect the roles of individual myeloid cell subsets in the survival of ECTV infection. We showed that DC are the primary producers of type I interferons (T1-IFN), requisite cytokines for survival, following ECTV infection. DC, but not macrophages, monocytes, or granulocytes, were required for control of the virus and survival of mice following ECTV infection. Depletion of either plasmacytoid DC (pDC) alone or the lymphoid-resident DC subset (CD8α+DC) alone did not confer lethal susceptibility to ECTV. However, the function of at least one of the pDC or CD8α+DC subsets is required for survival of ECTV infection, as mice depleted of both populations were susceptible to ECTV challenge. The presence of at least one of these DC subsets is sufficient for cytokine production that reduces ECTV replication and virus spread, facilitating survival following infection.IMPORTANCEPrior to the eradication of variola virus, the orthopoxvirus that causes smallpox, one-third of infected people succumbed to the disease. Following successful eradication of smallpox, vaccination rates with the smallpox vaccine have significantly dropped. There is now an increasing incidence of zoonotic orthopoxvirus infections for which there are no effective treatments. Moreover, the safety of the smallpox vaccine is of great concern, as complications may arise, resulting in morbidity. Like many viruses that cause significant human diseases, orthopoxviruses spread from a peripheral site of infection to become systemic. This study elucidates the early requirement for innate immune cells in controlling a peripheral infection with ECTV, the causative agent of mousepox. We report that there is redundancy in the function of two innate immune cell subsets in controlling virus spread early during infection. The viral control mediated by these cell subsets presents a potential target for therapies and rational vaccine design.