bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transepithelial corneal collagen cross‐linking by iontophoresis of riboflavin
Authors: Bikbova Guzel , Bikbov Mukharram 
Published: July 2013

Abstract.Purpose:  To evaluate the effectiveness of transepithelial cornea impregnation with riboflavin 0.1% by iontophoresis for collagen cross‐linking.Material and methods: Transepithelial collagen cross‐linking by iontophoresis of riboflavin was performed in a series of 22 eyes of 19 patients with progressive keratoconus I–II of Amsler classification. The riboflavin solution was administered by iontophoresis for 10 min in total, after which standard surface UVA irradiation (370 nm, 3 mW/cm2) was performed at a 5‐cm distance for 30 min.Results: The riboflavin/UVA treatment resulted in a decrease in the average keratometry level from 46.47 ± 1.03 to 44.12 ± 1.12 D 1 year after the procedure. Corneal astigmatism decreased from 3.44 ± 0.48 to 2.95 ± 0.23 D. Uncorrected distance visual acuity improved from 0.61 ± 0.44 up to 0.48 ± 0.41 (LogMAR). Preoperative and postoperative endothelial cell density remained unchanged at 2765 ± 21.15 cells/mm2.Conclusion: Transepithelial collagen cross‐linking by iontophoresis might become an effective method for riboflavin impregnation of the corneal stroma reducing the duration of the procedure and being more comfortable for the patients. Further long‐term studies are necessary to complete the evaluation of the efficacy and risk spectrum of the modified cross‐linking technique.
Effect of Danofloxacin on Reactive Oxygen Species Production, Lipid Peroxidation and Antioxidant Enzyme Activities in Kidney Tubular Epithelial Cell Line, LLC‐PK1
Authors: Yu Chun‐Hong , Liu Zhao‐Ying , Sun Lei‐Sheng , Li Yu‐Juan , Zhang Da‐Sheng , Pan Ren‐Tao , Sun Zhi‐Liang 
Published: July 2013

AbstractThe purpose of this study was to investigate the possibility that oxidative stress was involved in danofloxacin‐induced toxicity in renal tubular cells epithelial cell line (LLC‐PK1). Confluent LLC‐PK1 cells were incubated with various concentrations of danofloxacin. The extent of oxidative damage was assessed by measuring the reactive oxygen species (ROS) level, lipid peroxidation, cell apoptosis and antioxidative enzyme activities. Danofloxacin induced a concentration‐dependent increase in the ROS production, not even cytotoxic conditions. Similarly, danofloxacin caused an about 4 times increase in the level of thiobarbituric acid reactive substances at the concentration of 400 μM for 24 hr, but it did not induce cytotoxicity and apoptosis. Antioxidant enzymes activities, such as superoxide dismutase (SOD) and catalase (CAT), were increased after treatment with 100, 200 and 400 μM of danofloxacin for 24 hr. The activity of glutathione peroxidase (GPX) was significantly decreased in a concentration‐dependent manner. In addition, ROS production, lipid peroxidation and GPX decline were inhibited by additional glutathione and N‐acetyl cysteine. These data suggested that danofloxacin could not induce oxidative stress in LLC‐PK1 cells at the concentration (≤400 μM) for 24 hr. The increase levels of ROS and lipid peroxidation could be partly abated by the increase activities of SOD and CAT.
Efficacy and safety of pegylated‐interferon α‐2a in myelofibrosis: a study by the FIM and GEM French cooperative groups
Authors: Ianotto Jean‐Christophe , Boyer‐Perrard Françoise , Gyan Emmanuel , Laribi Kamel , Cony‐Makhoul Pascale , Demory Jean‐Loup , De Renzis Benoit , Dosquet Christine , Rey Jerome , Roy Lydia , Dupriez Brigitte , Knoops Laurent , Legros Laurence , Malou Mohamed , Hutin Pascal , Ranta Dana , Schoenwald Michele , Andreoli Annalisa , Abgrall Jean‐François , Kiladjian Jean‐Jacques 
Published: July 2013

SummaryMyeloproliferative neoplasm‐related myelofibrosis is associated with cytopenic or proliferative phases, splenomegaly and constitutional symptoms. Few effective treatments are available and small series suggested that interferon could be an option for myelofibrosis therapy. We performed a retrospective study of pegylated‐interferon α‐2a (Peg‐IFNα‐2a) therapy in myelofibrosis. Sixty‐two patients treated with Peg‐IFNα‐2a at 17 French and Belgian centres were included. Responses were determined based on the criteria established by the International Working Group for Myelofibrosis Research and Treatment. Mean follow‐up was 26 months. Sixteen of 25 anaemic patients (64%) (eight concomitantly receiving recombinant erythropoietin) achieved a complete response and transfusion‐independence was obtained in 5/13 patients (38·5%). Constitutional symptoms resolved in 82% of patients. All five leucopenic patients normalized their leucocyte counts, whereas a normal platelet count was obtained in 5/8 thrombocytopenic patients. Splenomegaly was reduced in 46·5% of patients, and complete resolution of thrombocytosis and leucocytosis were observed in 82·8% and 68·8% of patients, respectively. Side effects (mostly haematological) were mainly of grade 1–2. The only factor independently associated with treatment failure was a spleen enlargement of more than 6 cm below the costal margin. In conclusion, Peg‐IFNα‐2a induced high response rates with acceptable toxicity in a large proportion of patients with primary and secondary myelofibrosis, especially in early phases.
Catheter‐related thrombosis in cancer patients
Authors: Murray Jim , Precious Elizabeth , Alikhan Raza 
Published: July 2013

SummaryCentral venous catheters are commonly used in haematology departments for the administration of chemotherapy, blood products and parenteral nutrition in patients with haematological malignancy. Thrombosis is a recognized complication of such devices especially in oncology patients. Catheter‐related thrombi (CRT) may lead to pulmonary embolism and infection, as well as catheter failure and potential delays in treatment. The vast majority of CRT are asymptomatic, thus a high index of suspicion is required in making the diagnosis. Doppler ultrasound or venography may be employed to identify CRT. Once confirmed, the initiation of treatment is a balance between the risks of anticoagulation against potential complications of CRT. A number of risk factors for CRT are discussed along with an overview of catheter types, their appropriate choice and intravascular positioning.
Clinical outcome and predictors of survival in patients with pneumocystis jirovecii pneumonia – results of a tertiary referral centre
Authors: Roembke Felicitas , Heinzow Hauke Sebastian , Gosseling Thomas , Heinecke Achim , Domagk Dirk , Domschke Wolfram , Meister Tobias 
Published: July 2013

AbstractBackground and AimsPneumocystis jirovecii pneumonia also known as pneumocystis pneumonia (PCP) is an opportunistic respiratory infection in human immunodeficiency virus (HIV) patients that may also develop in non‐HIV immunocompromised persons. The aim of our study was to evaluate mortality predictors of PCP patients in a tertiary referral centre.MethodsFifty‐one patients with symptomatic PCP were enrolled in the study. The patients had either HIV infection (n = 21) or other immunosuppressive conditions (n = 30). Baseline characteristics (e.g. age, sex and underlying disease) were retrieved. Kaplan–Meier analysis was employed to calculate survival. Comparisons were made by log‐rank test. A multivariate analysis of factors influencing survival was carried out using the Cox regression model. Chi‐squared test and Wilcoxon–Mann–Whitney test was applied as appropriate.ResultsThe median survival time for the HIV group was >120 months compared with 3 months for the non‐HIV group (P = 0.009). Three‐month survival probability was also significantly greater in the HIV group compared with the non‐HIV group (90% vs 41%, P = 0.002). In univariate log‐rank test, intensive care unit (ICU) necessity, HIV negativity, age >50 years, haemoglobin <10g/dl, C‐reactive protein >5 mg/dL and multiple comorbidities were significant negative predictors of survival. In the Cox regression model, ICU and HIV statuses turned out to be independent prognostic factors of survival.ConclusionPCP is a serious problem in non‐HIV immunocompromised patients in whom survival outcomes are worse than those in HIV patients.
Providing Access to Engagement in Learning: The Potential of Universal Design for Learning in Museum Design
Authors: Rappolt‐Schlichtmann Gabrielle , Daley Samantha G. 
Published: July 2013

AbstractFollowing passage of the Americans with Disabilities Act of 1990 (ADA), many museums improved the accessibility of their facilities. Even so, individuals with disabilities still lag behind in participation and engagement in museum experiences. Universal Design for Learning (UDL) provides an alternate model for the design of museum programs and exhibit spaces, one that is more aligned to progressive concepts of disability, providing not only physical access but also access to engagement in learning. In this article we argue that UDL has the potential to substantially improve the design of informal learning environments. Through two illustrative examples, we describe how the UDL design guidelines can be used to improve the probability that engagement will occur as individuals interact with exhibits, programs, and people in museums.
Improving the Museum Experiences of Children with Autism Spectrum Disorders and Their Families: An Exploratory Examination of Their Motivations and Needs and Using Web‐based Resources to Meet Them
Authors: Langa Lesley A. , Monaco Pino , Subramaniam Mega , Jaeger Paul T. , Shanahan Katie , Ziebarth Beth 
Published: July 2013

AbstractThrough a partnership with a local school, the Smithsonian Institution and the Information Policy and Access Center at the University of Maryland conducted an exploratory study to examine the motivations and needs of families visiting museums with children with Autism Spectrum Disorders (ASDs). This study represents one of the first undertakings to study visitors with ASDs, especially children, through a primarily qualitative data collection. Interest‐driven enjoyment emerged as a primary motivation, though to relax and to socialize outside of the family boundaries were not ranked as important motives for visiting museums. Children, who were directly interviewed, gave positive assessments of their museum experiences, while parents commented that challenges, both museum‐ and family‐related (crowds, loud noise, not feeling welcome, and a child's unpredictable behavior) surfaced in public settings like museums. Parents desired a “typical family outing” with their ASDs child, stating that manageable and safe environments helped families experience a museum.
Play For All at Chicago Children's Museum: A History and Overview
Authors: Golden Tamara , Walsh Lynn 
Published: July 2013

AbstractIn 2004, Chicago Children's Museum founded the Play For All initiative, which creates a community where play and learning connect for visitors of all abilities. The Play For All team discovered that families with children with disabilities were looking for an opportunity to explore and enjoy the museum without limitations and the fear of being judged. CCM also learned that being ADA compliant was not enough; being accessible and inclusive meant going above and beyond the ADA. Children's museums are a rare treat where almost everything should be interactive and ready to be played with. The Play For All team has made significant changes to the exhibits, staff training, and programming at Chicago Children's Museum by working with local families with disabilities and partner organizations. A culture shift has taken place within CCM. Play For All is now a top priority when decisions are being made.
Using occupancy and species distribution models to assess the conservation status and habitat use of the goldline darter (Percina aurolineata) in Georgia, USA
Authors: Albanese Brett , Litts Thomas , Camp Mieko , Weiler Deborah A. 
Published: July 2013

AbstractThe goldline darter (Percina aurolineata) is threatened by recent increases in urbanisation in the Coosawattee River watershed, but no studies have addressed their current status. Similarly, limited data on habitat use make it difficult to understand factors that may impact this species. We compared occurrence data before (1996–2000) and after (2009–2011) a period of rapid development and population growth within the watershed. Single‐season occupancy models were built to account for imperfect species detection and to identify habitat covariates. MaxEnt was used to identify important stream reaches for conservation and to understand landscape scale factors correlated with the distribution of goldline darters. Our results indicate a high proportion of sites occupied upstream of Carters Lake during the historic and recent time periods, with no evidence of decline. However, occurrences of goldline darters for sites in Talking Rock Creek and the lower Coosawattee River were sparse in the historic period and absent during the recent period. The probability of detecting goldline darters is low and was positively associated with the occurrence of small substrate. Species distribution models were strongly influenced by watershed area and elevation and indicated a high probability of suitable habitat within the Coosawattee River and large tributaries upstream of Carters Lake. While goldline darter occupancy is currently stable upstream of Carters Lake, continued urbanisation is a threat to long‐term persistence. We recommend additional monitoring and describe a protocol that allows for precise estimates of species occupancy while minimising the risk of sampling‐related mortality.
Niche conservatism constrains Australian honeyeater assemblages in stressful environments
Authors: Miller E. T. , Zanne A. E. , Ricklefs R. E. 
Published: July 2013

AbstractThe hypothesis of phylogenetic niche conservatism proposes that most extant members of a clade remain in ancestral environments because expansion into new ecological space imposes a selectional load on a population. A prediction that follows is that local assemblages contain increasingly phylogenetically clustered subsets of species with increasing difference from the ancestral environment of a clade. We test this in Australian Meliphagidae, a continental radiation of birds that originated in wet, subtropical environments, but subsequently spread to drier environments as Australia became more arid during the late Cenozoic. We find local assemblages are increasingly phylogenetically clustered along a gradient of decreasing precipitation. The pattern is less clear along a temperature gradient. We develop a novel phyloclimatespace to visualise the expansion of some lineages into drier habitats. Although few species extend into arid regions, those that do occupy larger ranges and thus local species richness does not decline predictably with precipitation.
The ‘Dr Jekyll and Mr Hyde fungus’: noble rot versus gray mold symptoms of Botrytis cinerea on grapes
Authors: Fournier Elisabeth , Gladieux Pierre , Giraud Tatiana 
Published: July 2013

AbstractMany cryptic species have recently been discovered in fungi, especially in fungal plant pathogens. Cryptic fungal species co‐occurring in sympatry may occupy slightly different ecological niches, for example infecting the same crop plant but specialized on different organs or having different phenologies. Identifying cryptic species in fungal pathogens of crops and determining their ecological specialization are therefore crucial for disease management. Here, we addressed this question in the ascomycete Botrytis cinerea, the agent of gray mold on a wide range of plants. On grape, B. cinerea causes severe damage but is also responsible for noble rot used for processing sweet wines. We used microsatellite genotyping and clustering methods to elucidate whether isolates sampled on gray mold versus noble rot symptoms in three French regions belong to genetically differentiated populations. The inferred population structure matched geography rather than the type of symptom. Noble rot symptoms therefore do not seem to be caused by a specific B. cinerea population but instead seem to depend essentially on microclimatic conditions, which has applied consequences for the production of sweet wines.
Transport and survival of Japanese sardine (Sardinops melanostictus) eggs and larvae via particle‐tracking experiments
Authors: Nishikawa Haruka , Yasuda Ichiro , Itoh Sachihiko , Komatsu Kosei , Sasaki Hideharu , Sasai Yoshikazu , Oozeki Yoshioki 
Published: July 2013

AbstractParticle‐tracking experiments were performed to infer the distribution of larvae of the Japanese sardine (Sardinops melanostictus) and to detect effects of transport environment on sardine recruitment, using the output of a high‐resolution ocean general circulation model and observed data of sardine spawning grounds during 1978–2004. By the 60th day following spawning, approximately 50% of the larvae had been transported to the Kuroshio Extension (KE). Whereas the spawning period and grounds changed markedly in relation to the stock level, the proportion of larvae transported to the KE remained relatively constant and no significant correlations were found between sardine recruitment and the transport proportion. Instead, the recruitment was found to be correlated with physical parameters including the mixed layer depth and the sea surface temperature along several major transport trajectories of sardine larvae. The correlations were most significant for the trajectories in the region 0.5° south to 1° north of the Kuroshio axis (defined as the location of velocity maxima at each longitude) and for larvae spawned in February and March during the high stock period (1978–94), and for larvae spawned in March and April during the low stock period (1995–2004).
Role of compressive tectonics on gas charging into the Ordovician sandstone reservoirs in the Sbaa Basin, Algeria: constrained by fluid inclusions and mineralogical data
Authors: Wazir I. , Pagel M. , Tournier F. , Portier E. , Renac C. 
Published: July 2013

AbstractStructure‐ and tectonic‐related gas migration into Ordovician sandstone reservoirs and its impact on diagenesis history were reconstructed in two gas fields in the Sbaa Basin, in SW Algeria. This was accomplished by petrographical observations, fluid inclusion microthermometry and stable isotope geochemistry on quartz, dickite and carbonate cements and veins. Two successive phases of quartz cementation (CQ1 and CQ2) occurred in the reservoirs. Two phase aqueous inclusions show an increase in temperatures and salinities from the first CQ1 diagenetic phase toward CQ2 in both fields. Microthermometric data on gas inclusions in quartz veins reveal the presence of an average of 92 ± 5 mole% of CH4 considering a CH4‐CO2 system, which is similar to the present‐day gas composition in the reservoirs. The presence of primary methane inclusions in early quartz overgrowths and in quartz and calcite veins suggests that hydrocarbon migration into the reservoir occurred synchronically with early quartz cementation in the sandstones located near the contact with the Silurian gas source rock at 100–140°C during the Late Carboniferous period and the late Hercynian episode fracturing at temperatures between 117 and 185°C, which increased in the NW‐direction of the basin. During the fracture filling, three main types of fluids were identified with different salinities and formation temperatures. A supplementary phase of higher fluid temperature (up to 226°C) recorded in late quartz, and calcite veins is related to a Jurassic thermal event. The occurrence of dickite cements close to the Silurian base near the main fault areas in both fields is mainly correlated with the sandstones where the early gas was charged. It implies that dickite precipitation is related to acidic influx. Late carbonate cements and veins (calcite – siderite – ankerite and strontianite) occurred at the same depths resulting from the same groundwater precipitation. The absence of methane inclusions in calcite cements result from methane flushing by saline waters.
Acid saline fluid inclusions: examples from modern and Permian extreme lake systems
Authors: Benison K. C. 
Published: July 2013

AbstractSome of the Earth's most extreme aqueous chemistries are found at acid saline lakes and associated shallow groundwaters. Modern lakes and groundwaters in Western Australia have pHs as low as 1.5, total dissolved solids as high as 32%, unusually high concentrations of Al, Si, Fe, As, and Sb, and many other chemical characteristics atypical of natural waters. Analogous Permian red bed and evaporite lake deposits in the US midcontinent also had extreme water compositions, including negative pH. Fluid inclusions in chemical sediments from modern and Permian acid saline lakes record specific physical, chemical, and biological conditions. Primary inclusions in this bedded halite and gypsum are trapped shallow lake water, air bubbles, crystals of other minerals, and microorganisms and organic compounds. Isolated fluid inclusions in early diagenetic phases of halite are remnants of shallow groundwaters and can be analyzed to determine chemical compositions of past groundwaters. A variety of traditional methods, including petrography, freezing–melting microthermometry and laser Raman spectroscopy, and innovative variations, such as homogenization of artificially nucleated vapor bubbles and UV‐vis petrography, produce data detailing environmental conditions. Other traditionally used methods, such as laser ablation ICP‐MS, are not effective due to the inclusions' high and complex salinity, extremely low pH and/or low pressure. Making synthetic solutions that match the complexity of natural acid saline inclusions is another challenge. Fluid inclusions in acid‐precipitated halite have proven to be excellent repositories for past surface chemical, physical, and biological conditions. Although the methods used and data obtained from acid‐precipitated halite and gypsum are not exactly the same as for neutral‐alkaline evaporites, acid inclusions provide high‐resolution environmental data for modern and ancient extreme environments. These extreme fluids may also have similarities to other past extreme environments, to ore mineralizing fluids, and to past liquids on Mars.
STEAP1 protein overexpression is an independent marker for biochemical recurrence in prostate carcinoma
Authors: Ihlaseh‐Catalano Shadia M , Drigo Sandra A , de Jesus Carlos M N , Domingues Maria Aparecida C , Trindade Filho José Carlos S , de Camargo João Lauro V , Rogatto Silvia R 
Published: July 2013

AimsTo investigate the prognostic value of expression levels of the genes STEAP1 and STEAP2, and of STEAP1 protein, in prostate carcinomas (PCa).Methods and resultsSTEAP1 and STEAP2 transcript levels were evaluated by RT‐qPCR in samples from 35 PCa, 24 adjacent non‐neoplastic prostate (AdjP) tissues, five cases of benign prostatic hyperplasia (BPH), and two histologically normal prostates (N). STEAP1 expression was assessed by immunohistochemistry in samples from 198 PCa, 76 AdjP, 22 BPH, and two N. The findings were compared with clinical and pathological parameters and patient outcome. STEAP1 and STEAP2 transcript analysis showed no differences between the groups tested. Although not significant, higher STEAP1 mRNA levels were detected in tumours with high Gleason scores and in patients who presented with biochemical recurrence (BCR). STEAP1 overexpression was detected in PCa, and was significantly associated with high‐grade Gleason scores, seminal vesicle invasion, BCR, and worse outcome (metastasis or PCa‐specific death). STEAP1 overexpression was significantly associated with shorter BCR‐free survival. Multivariate analysis revealed that STEAP1 is an independent marker for BCR.ConclusionsThese findings provide evidence that STEAP1 is a biomarker of worse prognosis in PCa patients.