bluuebunny/crossref_metadata_2025_split huggingface dataset:
TORC1 controls G1–S cell cycle transition in yeast via Mpk1 and the greatwall kinase pathway
Authors: Moreno-Torres Marta , Jaquenoud Malika , De Virgilio Claudio 
Published: September 2015

AbstractThe target of rapamycin complex 1 (TORC1) pathway couples nutrient, energy and hormonal signals with eukaryotic cell growth and division. In yeast, TORC1 coordinates growth with G1–S cell cycle progression, also coined as START, by favouring the expression of G1 cyclins that activate cyclin-dependent protein kinases (CDKs) and by destabilizing the CDK inhibitor Sic1. Following TORC1 downregulation by rapamycin treatment or nutrient limitation, clearance of G1 cyclins and C-terminal phosphorylation of Sic1 by unknown protein kinases are both required for Sic1 to escape ubiquitin-dependent proteolysis prompted by its flagging via the SCFCdc4 (Skp1/Cul1/F-box protein) ubiquitin ligase complex. Here we show that the stabilizing phosphorylation event within the C-terminus of Sic1 requires stimulation of the mitogen-activated protein kinase, Mpk1, and inhibition of the Cdc55 protein phosphatase 2A (PP2ACdc55) by greatwall kinase-activated endosulfines. Thus, Mpk1 and the greatwall kinase pathway serve TORC1 to coordinate the phosphorylation status of Sic1 and consequently START with nutrient availability.
Transgranular liquation cracking of grains in the semi-solid state
Authors: Karagadde S. , Lee P. D. , Cai B. , Fife J. L. , Azeem M. A. , Kareh K. M. , Puncreobutr C. , Tsivoulas D. , Connolley T. , Atwood R. C. 
Published: September 2015

AbstractGrain refinement via semi-solid deformation is desired to obtain superior mechanical properties of cast components. Using quantitative in situ synchrotron X-ray tomographic microscopy, we show an additional mechanism for the reduction of grain size, via liquation assisted transgranular cracking of semi-solid globular microstructures. Here we perform localized indentation of Al-15wt.%Cu globular microstructures, with an average grain size of ∼480 μm, at 555 °C (74% solid fraction). Although transgranular fracture has been observed in brittle materials, our results show transgranular fracture can also occur in metallic alloys in semi-solid state. This transgranular liquation cracking (TLC) occurs at very low contact stresses (between 1.1 and 38 MPa). With increasing strain, TLC continues to refine the size of the microstructure until the grain distribution reaches log-normal packing. The results demonstrate that this refinement, previously attributed to fragmentation of secondary arms by melt-shearing, is also controlled by an additional TLC mechanism.
The Janus face of Darwinian competition
Authors: Hintze Arend , Phillips Nathaniel , Hertwig Ralph 
Published: September 2015

AbstractWithout competition, organisms would not evolve any meaningful physical or cognitive abilities. Competition can thus be understood as the driving force behind Darwinian evolution. But does this imply that more competitive environments necessarily evolve organisms with more sophisticated cognitive abilities than do less competitive environments? Or is there a tipping point at which competition does more harm than good? We examine the evolution of decision strategies among virtual agents performing a repetitive sampling task in three distinct environments. The environments differ in the degree to which the actions of a competitor can affect the fitness of the sampling agent and in the variance of the sample. Under weak competition, agents evolve decision strategies that sample often and make accurate decisions, which not only improve their own fitness, but are good for the entire population. Under extreme competition, however, the dark side of the Janus face of Darwinian competition emerges: Agents are forced to sacrifice accuracy for speed and are prevented from sampling as often as higher variance in the environment would require. Modest competition is therefore a good driver for the evolution of cognitive abilities and of the population as a whole, whereas too much competition is devastating.
Graphene-Wrapped Anatase TiO2 Nanofibers as High-Rate and Long-Cycle-Life Anode Material for Sodium Ion Batteries
Authors: Yeo Yeolmae , Jung Ji-Won , Park Kyusung , Kim Il-Doo 
Published: September 2015

AbstractAnatase TiO2 has been suggested as a potential sodium anode material, but the low electrical conductivity of TiO2 often limits the rate capability, resulting in poor electrochemical properties. To address this limitation, we propose graphene-wrapped anatase TiO2 nanofibers (rGO@TiO2 NFs) through an effective wrapping of reduced graphene oxide (rGO) sheets on electrospun TiO2 NFs. To provide strong electrostatic interaction between the graphene oxide (GO) sheets and the TiO2 NFs, poly(allylamine hydrochloride) (PAH) was used to induce a positively charged TiO2 surface by the immobilization of the -NH3+ group and to promote bonding with the negatively charged carboxylic acid (-COO−) and hydroxyl (-O−) groups on the GO. A sodium anode electrode using rGO@TiO2 NFs exhibited a significantly improved initial capacity of 217 mAh g−1, high capacity retention (85% after 200 cycles at 0.2C) and a high average Coulombic efficiency (99.7% from the second cycle to the 200th cycle), even at a 5C rate, compared to those of pristine TiO2 NFs. The improved electrochemical performances stem from highly conductive properties of the reduced GO which is effectively anchored to the TiO2 NFs.
Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system
Authors: Wang Xiaolong , Yu Honghao , Lei Anmin , Zhou Jiankui , Zeng Wenxian , Zhu Haijing , Dong Zhiming , Niu Yiyuan , Shi Bingbo , Cai Bei , Liu Jinwang , Huang Shuai , Yan Hailong , Zhao Xiaoe , Zhou Guangxian , He Xiaoling , Chen Xiaoxu , Yang Yuxin , Jiang Yu , Shi Lei , Tian Xiue , Wang Yongjun , Ma Baohua , Huang Xingxu , Qu Lei , Chen Yulin 
Published: September 2015

AbstractRecent advances in the study of the CRISPR/Cas9 system have provided a precise and versatile approach for genome editing in various species. However, the applicability and efficiency of this method in large animal models, such as the goat, have not been extensively studied. Here, by co-injection of one-cell stage embryos with Cas9 mRNA and sgRNAs targeting two functional genes (MSTN and FGF5), we successfully produced gene-modified goats with either one or both genes disrupted. The targeting efficiency of MSTN and FGF5 in cultured primary fibroblasts was as high as 60%, while the efficiency of disrupting MSTN and FGF5 in 98 tested animals was 15% and 21% respectively and 10% for double gene modifications. The on- and off-target mutations of the target genes in fibroblasts, as well as in somatic tissues and testis of founder and dead animals, were carefully analyzed. The results showed that simultaneous editing of several sites was achieved in large animals, demonstrating that the CRISPR/Cas9 system has the potential to become a robust and efficient gene engineering tool in farm animals and therefore will be critically important and applicable for breeding.
Hybridization between alien species Rumex obtusifolius and closely related native vulnerable species R. longifolius in a mountain tourist destination
Authors: Takahashi Koichi , Hanyu Masaaki 
Published: September 2015

AbstractAlien species expand their distribution by transportation network development. Hybridization between alien species Rumex obtusifolius and closely related native vulnerable species R. longifolius was examined in a mountain tourist destination in central Japan. The three taxa were morphologically identified in the field. Stem height and leaf area were greater in R. longifolius than R. obtusifolius; hybrids were intermediate between the two Rumex species. R. longifolius and the hybrids grew mainly in wet land and the river tributary; R. obtusifolius grew mainly at the roadside and in meadows. Hybrid germination rates of pollen and seeds were much lower than for the two Rumex species. Clustering analysis showed the three taxa each formed a cluster. Most hybrids were F1 generation; the possibility was low of introgression into the two Rumex species by backcross. This study clarified that (1) hybridization occurred between R. obtusifolius and R. longifolius because they occurred together in a small area, but grew in different water habitat conditions and (2) hybridization was mostly F1 generation because hybrid pollen and seed fertility was low. However, we need caution about introgression into R. longifolius by R. obtusifolius in this area because of the slight possibility of F2 generation and backcrosses.
Control of optical spin Hall shift in phase-discontinuity metasurface by weak value measurement post-selection
Authors: Lee Y.U. , Wu J.W. 
Published: September 2015

AbstractSpin Hall effect of light is a spin-dependent transverse shift of optical beam propagating along a curved trajectory, where the refractive index gradient plays a role of the electric field in spin Hall effect of solid-state systems. In order to observe optical spin Hall shift in a refraction taking place at air-glass interface, an amplification technique was necessary such as quantum weak measurement. In phase-discontinuity metasurface (PMS) a rapid phase-change along metasurface takes place over subwavelength distance, which leads to a large refractive index gradient for refraction beam enabling a direct detection of optical spin Hall shift without amplification. Here, we identify that the relative optical spin Hall shift depends on incidence angle at PMS and demonstrate a control of optical spin Hall shift by constructing weak value measurement with a variable phase retardance in the post-selection. Capability of optical spin Hall shift control permits a tunable precision metrology applicable to nanoscale photonics such as angular momentum transfer and sensing.
Occurrence of chiral organochlorine compounds in the environmental matrices from King George Island and Ardley Island, west Antarctica
Authors: Wang Pu , Zhang Qinghua , Li Yingming , Zhu Chaofei , Chen Zhaojing , Zheng Shucheng , Sun Huizhong , Liang Yong , Jiang Guibin 
Published: September 2015

AbstractChiral organochlorine compounds (OCs) were measured in various environmental matrices (air, soil and vegetation) from west Antarctica using high resolution gas chromatography coupled with high resolution mass spectrometry (HRGC/HRMS). They were generally detected at a global background level compared with the previous studies. α-HCH and PCB-183 was observed in all the matrices except PCB-183 in two soil samples, while PCB-95, -136, -149, -174, -176 and o,p’-DDT were detected in most air but only a few solid matrices. Enantiomeric fractions (EFs) indicated that nonracemic residues of chiral OCs occurred in all the matrices and a wide variation of the EF values was observed in the vegetation. There was significant discrepancy between the EF values of PCB-183 and the racemic values, indicating that stereoselective depletion of PCB-183 was probably associated with the water-air exchange. The EFs values of α-HCH were generally lower than the racemic values but no statistical difference was obtained in all the matrices except lichen, supporting the assumption that water-air exchange may make influence on long-range transport of α-HCH.
Importance of liquid fragility for energy applications of ionic liquids
Authors: Sippel P. , Lunkenheimer P. , Krohns S. , Thoms E. , Loidl A. 
Published: September 2015

AbstractIonic liquids (ILs) are salts that are liquid close to room temperature. Their possible applications are numerous, e.g., as solvents for green chemistry, in various electrochemical devices and even for such “exotic” purposes as spinning-liquid mirrors for lunar telescopes. Here we concentrate on their use for new advancements in energy-storage and -conversion devices: Batteries, supercapacitors or fuel cells using ILs as electrolytes could be important building blocks for the sustainable energy supply of tomorrow. Interestingly, ILs show glassy freezing and the universal, but until now only poorly understood dynamic properties of glassy matter, dominate many of their physical properties. We show that the conductivity of ILs, an essential figure of merit for any electrochemical application, depends in a systematic way not only on their glass temperature but also on the so-called fragility, characterizing the non-canonical super-Arrhenius temperature dependence of their ionic mobility.
High-throughput sequencing and morphology perform equally well for benthic monitoring of marine ecosystems
Authors: Lejzerowicz Franck , Esling Philippe , Pillet Loïc , Wilding Thomas A. , Black Kenneth D. , Pawlowski Jan 
Published: September 2015

AbstractEnvironmental diversity surveys are crucial for the bioassessment of anthropogenic impacts on marine ecosystems. Traditional benthic monitoring relying on morphotaxonomic inventories of macrofaunal communities is expensive, time-consuming and expertise-demanding. High-throughput sequencing of environmental DNA barcodes (metabarcoding) offers an alternative to describe biological communities. However, whether the metabarcoding approach meets the quality standards of benthic monitoring remains to be tested. Here, we compared morphological and eDNA/RNA-based inventories of metazoans from samples collected at 10 stations around a fish farm in Scotland, including near-cage and distant zones. For each of 5 replicate samples per station, we sequenced the V4 region of the 18S rRNA gene using the Illumina technology. After filtering, we obtained 841,766 metazoan sequences clustered in 163 Operational Taxonomic Units (OTUs). We assigned the OTUs by combining local BLAST searches with phylogenetic analyses. We calculated two commonly used indices: the Infaunal Trophic Index and the AZTI Marine Biotic Index. We found that the molecular data faithfully reflect the morphology-based indices and provides an equivalent assessment of the impact associated with fish farms activities. We advocate that future benthic monitoring should integrate metabarcoding as a rapid and accurate tool for the evaluation of the quality of marine benthic ecosystems.
Ability paradox of cascading model based on betweenness
Authors: Wang Jianwei , Xu Bo , Wu Yuedan 
Published: September 2015

AbstractMust Investing more resources to protect every node in a network improve the robustness of the whole network subject to target attacks? To answer this question, we investigate the cascading dynamics in some typical networks. In real networks, the load on a node is generally correlated with the betweenness. Considering the weight of a node, we give a new method to define the initial load on a node by the revised betweenness. Then we present a simple cascading model. We investigate the cascading dynamics by disabling a single key node with the highest load. We find that in BA scale-free networks, the bigger the capacity of every node, the stronger the robustness of the whole network. However, in WS networks and some random networks, when we increase the capacity of every node, instead, the robustness of the whole network is weaker. In US power grid and the China power grid, we also observe this counterintuitive phenomenon. We give a reasonable explanation by a simple illusion. By the analysis, we think that resurrections of some nodes in a ring network structure after removing a node may be the reason of this phenomenon.
EGFR Exon 18 Mutations in East Asian Patients with Lung Adenocarcinomas: A Comprehensive Investigation of Prevalence, Clinicopathologic Characteristics and Prognosis
Authors: Cheng Chao , Wang Rui , Li Yuan , Pan Yunjian , Zhang Yang , Li Hang , Zheng Difan , Zheng Shanbo , Shen Xuxia , Sun Yihua , Chen Haiquan 
Published: September 2015

AbstractOur aim was to investigate the clinical and pathologic characteristics of the epidermal growth factor receptor (EGFR) exon 18 mutations in East Asian lung adenocarcinomas patients. A total of 1,201 lung adenocarcinomas were analyzed for mutation in EGFR. Clinical and pathologic characteristics of patients with EGFR exon 18 mutations were compared with those who harbored classic activating mutations (exon 19 deletions and the L858R point mutation). The mutations in EGFR exon 18 were observed in 2.8% of 1,201 lung adenocarcinomas and 4.6% of patients with EGFR mutations. Patients with a single EGFR exon of 18 mutations had a worse overall survival than those harboring the complex EGFR exon of 18 mutations (p = 0.002) or those with classic activating mutations (p = 0.014). Four of five patients with EGFR exon 18 mutations showed objective response to the EGFR-TKI therapies after disease recurrence. Our results demonstrated that single EGFR exon 18 mutations may be an indicator of poor prognosis compared with complex EGFR exon 18 mutations or classic mutations. Furthermore, the results of the current study will be helpful for decision-making in the treatment of patients with EGFR exon 18 mutations.
Parallel molecular routes to cold adaptation in eight genera of New Zealand stick insects
Authors: Dennis Alice B. , Dunning Luke T. , Sinclair Brent J. , Buckley Thomas R. 
Published: September 2015

AbstractThe acquisition of physiological strategies to tolerate novel thermal conditions allows organisms to exploit new environments. As a result, thermal tolerance is a key determinant of the global distribution of biodiversity, yet the constraints on its evolution are not well understood. Here we investigate parallel evolution of cold tolerance in New Zealand stick insects, an endemic radiation containing three montane-occurring species. Using a phylogeny constructed from 274 orthologous genes, we show that stick insects have independently colonized montane environments at least twice. We compare supercooling point and survival of internal ice formation among ten species from eight genera and identify both freeze tolerance and freeze avoidance in separate montane lineages. Freeze tolerance is also verified in both lowland and montane populations of a single, geographically widespread, species. Transcriptome sequencing following cold shock identifies a set of structural cuticular genes that are both differentially regulated and under positive sequence selection in each species. However, while cuticular proteins in general are associated with cold shock across the phylogeny, the specific genes at play differ among species. Thus, while processes related to cuticular structure are consistently associated with adaptation for cold, this may not be the consequence of shared ancestral genetic constraints.
Complex Quantum Network Manifolds in Dimension d > 2 are Scale-Free
Authors: Bianconi Ginestra , Rahmede Christoph 
Published: September 2015

AbstractIn quantum gravity, several approaches have been proposed until now for the quantum description of discrete geometries. These theoretical frameworks include loop quantum gravity, causal dynamical triangulations, causal sets, quantum graphity and energetic spin networks. Most of these approaches describe discrete spaces as homogeneous network manifolds. Here we define Complex Quantum Network Manifolds (CQNM) describing the evolution of quantum network states and constructed from growing simplicial complexes of dimension "Equation missing". We show that in d = 2 CQNM are homogeneous networks while for d > 2 they are scale-free i.e. they are characterized by large inhomogeneities of degrees like most complex networks. From the self-organized evolution of CQNM quantum statistics emerge spontaneously. Here we define the generalized degrees associated with the "Equation missing"-faces of the "Equation missing"-dimensional CQNMs and we show that the statistics of these generalized degrees can either follow Fermi-Dirac, Boltzmann or Bose-Einstein distributions depending on the dimension of the "Equation missing"-faces.
Rhein antagonizes P2X7 receptor in rat peritoneal macrophages
Authors: Hu Fen , Xing Fulin , Zhu Ge , Xu Guangxue , Li Cunbo , Qu Junle , Lee Imshik , Pan Leiting 
Published: September 2015

AbstractP2X7 receptor plays important roles in inflammation and immunity and thereby it serves as a potential therapeutic target for inflammatory diseases. Rhein, an anthraquinone derivative, exhibits significant anti-inflammatory and immunosuppressive activities in therapy. However, the underlying mechanisms are largely unclear. Here, we aimed to investigate the effects of rhein on P2X7 receptor-mediated responses in vitro. In HEK293 cells expressing rat P2X7 receptor, we first found that rhein concentration-dependently blocked ATP-induced cytosolic calcium concentration ([Ca2+]c) elevation and pore formation of the plasma membrane, two hallmarks of the P2X7 receptor activation. These two inhibitory effects of rhein were also observed in rat peritoneal macrophages. Furthermore, rhein counteracted macrophage phagocytosis attenuation and suppressed reactive oxygen species (ROS) production triggered by ATP/BzATP. Meanwhile, rhein reduced ATP/BzATP-induced IL-1β release in lipopolysaccharide-activated macrophages. Prolonged application of ATP caused macrophage apoptosis, while the presence of rhein suppressed this cell cytotoxicity. Such ATP/BzATP-induced cellular reactions were also inhibited by a well-known rat P2X7 receptor antagonist, brilliant blue G, in a similar way to rhein. Together, our results demonstrate that rhein inhibit ATP/BzATP-induced [Ca2+]c increase, pore formation, ROS production, phagocytosis attenuation, IL-1β release and cell apoptosis by antagonizing the P2X7 receptor in rat peritoneal macrophages.
Loss of G2 subunit of vacuolar-type proton transporting ATPase leads to G1 subunit upregulation in the brain
Authors: Kawamura Nobuyuki , Sun-Wada Ge-Hong , Wada Yoh 
Published: September 2015

AbstractVacuolar-type ATPase (V-ATPase) is a primary proton pump with versatile functions in various tissues. In nerve cells, V-ATPase is required for accumulation of neurotransmitters into secretory vesicles and subsequent release at the synapse. Neurons express a specific isoform (G2) of the G subunit of V-ATPase constituting the catalytic sector of the enzyme complex. Using gene targeting, we generated a mouse lacking functional G2 (G2 null), which showed no apparent disorders in architecture and behavior. In the G2-null mouse brain, a G1 subunit isoform, which is ubiquitously expressed in neuronal and non-neuronal tissues, accumulated more abundantly than in wild-type animals. This G1 upregulation was not accompanied by an increase in mRNA. These results indicate that loss of function of neuron-specific G2 isoform was compensated by an increase in levels of the G1 isoform without apparent upregulation of the G1 mRNA.
Mortality from contact-related epidemics among indigenous populations in Greater Amazonia
Authors: Walker Robert S. , Sattenspiel Lisa , Hill Kim R. 
Published: September 2015

AbstractEuropean expansion and contact with indigenous populations led to catastrophic depopulation primarily through the introduction of novel infectious diseases to which native peoples had limited exposure and immunity. In the Amazon Basin such contacts continue to occur with more than 50 isolated indigenous societies likely to make further contacts with the outside world in the near future. Ethnohistorical accounts are useful for quantifying trends in the severity and frequency of epidemics through time and may provide insight into the likely demographic consequences of future contacts. Here we compile information for 117 epidemics that affected 59 different indigenous societies in Greater Amazonia and caused over 11,000 deaths between 1875 and 2008, mostly (75%) from measles, influenza and malaria. Results show that mortality rates from epidemics decline exponentially through time and, independently, with time since peaceful contact. The frequency of documented epidemics also decreases with time since contact. While previous work on virgin soil epidemics generally emphasizes the calamity of contacts, we focus instead on improvements through time. The prospects for better survivorship during future contacts are good provided modern health care procedures are implemented immediately.