bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioengineered trachea using autologous chondrocytes for regeneration of tracheal cartilage in a rabbit model
Authors: Nomoto Mika , Nomoto Yukio , Tada Yasuhiro , Tani Akiko , Otsuki Koshi , Suzuki Ryo , Nakamura Tatsuo , Omori Koichi 
Published: July 2013

Objectives/HypothesisIn this study, a bioengineered trachea composed of autologous chondrocytes was developed, and its effect on cartilaginous regeneration was evaluated by implantation into tracheal defects in rabbits.Study DesignProspective controlled trial in an animal model.MethodsThe tracheal prosthesis used in this study was composed of polypropylene (the frame) and collagen sponge (the scaffold). Chondrocytes were harvested from the costal cartilage of rabbits and seeded into the tracheal prosthesis. The bioengineered trachea, consisting of the tracheal prosthesis with chondrocytes, was implanted into surgically created tracheal defects of rabbits in the bioengineered group, and a tracheal prosthesis without chondrocytes was implanted in the control group.ResultsAfter implantation, the presence of regenerated cartilage was observed in the bioengineered trachea but not in the tracheal prosthesis without chondrocytes.ConclusionsIt was confirmed in this study that the implanted chondrocytes proliferated in an appropriate portion of the tracheal defect and that the partially resected tracheal cartilage was repaired with regenerated cartilaginous tissue into a ring‐shaped form as a whole. These results demonstrate the feasibility of cartilaginous regeneration using a bioengineered trachea with autologous chondrocytes Laryngoscope, 123:2195–2201, 2013Level of EvidenceNA
Usefulness of the anterolateral thigh flap with vascularized fascia lata for reconstruction of orbital floor and nasal surface after total maxillectomy
Authors: Joo Young‐Hoon , Cho Kwang‐Jae , Park Jun‐Ook , Kim Min‐Sik 
Published: July 2013

Objectives/HypothesisThe aim of this study was to describe a technique using the fascia lata (FL) component of the anterolateral thigh (ALT) flap to re‐create the orbital floor and lateral nasal wall after total maxillectomy.Study DesignRetrospective analysis of medical records.MethodsA total of 22 patients underwent maxillary reconstruction using a composite ALT‐FL flap following cancer resection. All patients underwent total maxillectomies via the Weber–Ferguson approach. The ALT flap was harvested with the deep fascia of the thigh with the aim of using it for lining of the orbital floor and lateral nasal cavity. The FL was sutured to the palatine bone inferiorly, nasal bone and zygomatic bone superiorly, and nasopharyngeal mucosa posteriorly to provide an orbital floor and make a neonasal cavity.ResultsThere was 100% free flap survival. Speech was normal in eight (36%) patients, near normal in 10 (46%), and intelligible in four (18%). Seventeen (77%) patients gained a good facial appearance, and five (23%) a fair appearance. Sixteen (73%) patients complained of mild nasal crust formation, and the rest (27%) developed moderate crust.ConclusionsMicrovascular reconstruction using a composite ALT‐FL flap provided a reliable fascial component for orbital floor and nasal surface reconstruction of total maxillectomy defects.Level of Evidence4. Laryngoscope, 123:2125–2130, 2013
Role of endothelin a receptor in colon cancer metastasis: In vitro and in vivo evidence
Authors: Nie Shaolin , Zhou Jumei , Bai Fei , Jiang Bonian , Chen Juying , Zhou Jianping 
Published: July 2013

AbstractThe endothelin (ET)‐1/endothelin A receptor (ETAR) axis is reportedly involved in tumor cell invasion, survival, and metastasis. However, the role of ETAR in colon cancer metastasis and the underlying mechanisms have not been defined. In the present study, we assessed the role of ETAR in colon cancer metastasis in vitro and in vivo. Overexpression and knockdown of ETAR were respectively performed in SW480 and SW620 human colon cancer cells. Overexpression of ETAR in SW480 cells significantly increased cell survival against cisplatin, cell invasion, and matrix metalloproteinase (MMP)‐2 expression, which was strengthened by exogenous ET‐1 and abolished by selective ETAR antagonist BQ123 and phosphatidylinositol 3‐kinase (PI3K) inhibitor LY294002. Knockdown of ETAR in SW620 cells markedly decreased cell survival against cisplatin, cell invasion, and MMP‐2 expression, which was strengthened by BQ123 and LY294002, and partially rescued by exogenous ET‐1. In a colon cancer liver metastasis mouse model, while ETAR overexpression promoted colon cancer liver metastases, ETAR knockdown markedly decreased liver metastases. In conclusion, our in vitro data demonstrate that ETAR mediates the promoting effects of ET‐1 on colon cancer cell survival, invasion and MMP‐2 expression by a PI3K‐mediated mechanism. Our in vivo data indicate that ETAR markedly promotes colon cancer liver metastasis. This study provides direct evidence for a critical role of ETAR in colon cancer metastasis, which suggests that ETAR antagonism could benefit patients with metastatic colon cancer. © 2013 Wiley Periodicals, Inc.
Hypoxia regulates the sperm associated antigen 4 (SPAG4) via HIF, which is expressed in renal clear cell carcinoma and promotes migration and invasion in vitro
Authors: Knaup Karl Xaver , Monti Juliana , Hackenbeck Thomas , Jobst‐Schwan Tilmann , Klanke Bernd , Schietke Ruth Elisabeth , Wacker Ingrid , Behrens Juergen , Amann Kerstin , Eckardt Kai‐Uwe , Warnecke Christina , Wiesener Michael Sean 
Published: July 2013

AbstractHypoxia leads to the upregulation of a variety of genes mediated largely via the hypoxia inducible transcription factor (HIF). Prominent HIF‐regulated target genes such as the vascular endothelial growth factor (VEGF), the glucose transporter 1 (Glut‐1), or erythropoietin (EPO) help to assure survival of cells and organisms in a low oxygenated environment. Here, we are the first to report the hypoxic regulation of the sperm associated antigen 4 (SPAG4). SPAG4 is a member of the cancer testis (CT) gene family and to date little is known about its physiological function or its involvement in tumor biology. A number of CT family candidate genes are therefore currently being investigated as potential cancer markers, due to their predominant testicular expression pattern. We analyzed RNA and protein expression by RNAse protection assay, immunoflurescent as well as immunohistological stainings. To evaluate the influence of SPAG4 on migration and invasion capabilities, siRNA knockdown as well as transient overexpression was performed prior to scratch or invasion assay analysis. The hypoxic regulation of SPAG4 is clearly mediated in a HIF‐1 and VHL dependent manner. We furthermore show upregulation of SPAG4 expression in human renal clear cell carcinoma (RCC) and co‐localization within the nucleolus in physiological human testis tissue. SPAG4 knockdown reduces the invasion capability of RCC cells in vitro and overexpression leads to enhancement of tumor cell migration. Together, SPAG4 could possibly play a role in the invasion capability and growth of renal tumors and could represent an interesting target for clinical intervention. © 2013 Wiley Periodicals, Inc.
Homogenous fat suppression for bilateral breast imaging using independent shims
Authors: Han Misung , Cunningham Charles H. , Pauly John M. , Daniel Bruce L. , Hargreaves Brian A. 
Published: July 2013

AbstractPurposeTo demonstrate the capability of incorporating independent shims into a dual‐band spectral‐spatial excitation and to compare fat suppression between standard global shims and independent shims for in vivo bilateral breast imaging at 1.5T.MethodsA dual‐band spectral‐spatial excitation pulse was designed by interleaving two flyback spectral‐spatial pulses, playing one during positive gradient lobes and the other during negative gradient lobes. Each slab was enabled to have an independent spatial offset, spectral offset, and slab‐phase modulation by modulating radiofrequency phase, and independent linear shims were incorporated by playing extra shim gradients. Phantom experiments were performed to demonstrate the functionality of the pulse, and in vivo experiments were performed for 10 healthy volunteers to compare fat suppression between standard shims and independent shims.ResultsThe phantom experiments confirmed that the dual‐band pulse can provide independent spectral and spatial offsets and linear shims to the two slabs. Independent shims provided qualitatively more homogeneous fat suppression than standard shims in seven out of 10 subjects, with equivalent fat suppression in two of the other three subjects.ConclusionIncorporating independent shims into the dual‐band spectral‐spatial excitation can provide homogeneous fat suppression in bilateral breast imaging. Magn Reson Med 71:1511–1517, 2014. © 2013 Wiley Periodicals, Inc.
Getrennt und geschützt mit flüssigen Salzen
Authors: Marquardt Dorothea , Janiak Christoph 
Published: July 2013

AbstractMetallnanopartikel tendieren in Lösung dazu, sich zusammenzuballen. Ionische Flüssigkeiten bewahren sie davor. In diesen lassen sich daher besonders gut Metallverbindungen zu Nanopartikeln zersetzen. Elegant und schnell geht das mit Mikrowellenstrahlung.
Universitäten im globalen wirtschaftlichen Wettbewerb
Authors: Cammann Karl 
Published: July 2013

AbstractDer Aufschwung der chemischen Industrie in Deutschland basierte zum großen Teil auf einer regen Patentierungsstrategie. Die Forschung und Lehre an Hochschulen sollte einem frühzeitigen Schutz des geistigen Eigentums mehr Beachtung schenken.
Über einen heimlichen Gewinner
Authors: Herold Sabine 
Published: July 2013

AbstractJeder kennt ihn und jeder nutzt ihn, aber kaum jemand weiß ihn richtig einzusetzen: den Klebstoff. Spezialist für ihn ist ein mittelständisches Unternehmen, ein Hidden Champion: Delo. Wie wurde es, was es ist, und welche Strategie sichert seine Zukunft?
Das Jahr 2012: Alles in allem gut
Authors: Bulmahn Maren 
Published: July 2013

AbstractDie deutsche chemische Industrie schloss das vergangene Jahr zu großen Teilen zufrieden ab. Die Prognosen für das laufende Jahr sind weder euphorisch, noch ängstlich. Mancher deutsche Chef beneidet US‐amerikanische Unternehmen wegen ihrer geringen Energiekosten; das Vorkrisenniveau hat die US‐Wirtschaft aber noch nicht wieder erreicht.
Ultrasound and early tape mobilization—A practical solution for treating postoperative voiding dysfunction
Authors: Rautenberg Oliver , Kociszewski Jacek , Welter JoEllen , Kuszka Andrzej , Eberhard Jakob , Viereck Volker 
Published: July 2013

AbstractAimsThis study assessed the effectiveness of ultrasound in determining tape distance to urethra and the impact of early tape mobilization on outcomes in women with postoperative voiding dysfunction resulting from a too tightly positioned tension‐free vaginal tape (TVT).MethodsA prospective observational study was conducted with women experiencing voiding dysfunction caused by too tightly positioned tapes. Ultrasound was used to identify the cause of the dysfunction and measure the distance between tape and longitudinal smooth muscle layer (LSM) of the urethra. If the tape was too close to the LSM (<3 mm) and the residual volume was >100 ml, it was mobilized under local/analgosedation shortly after the initial TVT procedure.ResultsSeventy‐one postoperative TVT mobilization procedures were conducted on 61 women, which was 4.1% (61/1501) of all suburethral tape procedures performed. Early tape mobilization restored normal micturition in 59 (96.7%) of the women at the time of discharge. Significant differences were found in residual volumes (P < 0.001) and tape‐LSM distances (P < 0.001) pre‐ and post‐mobilization. At 6‐month follow‐up visits, 58 (95.1%) women were cured of SUI, three were incontinent, and no additional voiding dysfunction occurred.ConclusionsImmediate postoperative ultrasound can reliably detect too tightly positioned tapes that can be promptly treated with tape mobilization, a short and safe procedure that does not compromise the outcome of the original procedure. Neurourol. Urodynam. 33:1147–1151, 2014. © 2013 Wiley Periodicals, Inc.
Longitudinal weight gain in women identified With polycystic ovary syndrome: Results of an observational study in young women
Authors: Teede Helena J. , Joham Anju E. , Paul Eldho , Moran Lisa J. , Loxton Deborah , Jolley Damien , Lombard Catherine 
Published: July 2013

ObjectivePolycystic ovary syndrome (PCOS) affects 6‐18% of women. The natural history of weight gain in women with PCOS has not been well described. Here we aimed to examine longitudinal weight gain in women with and without PCOS and to assess the association between obesity and PCOS prevalence.Design and MethodsThe observational study was set in the general community. Participants were women randomly selected from the national health insurance scheme (Medicare) database. Mailed survey data were collected by the Australian Longitudinal Study on Women's Health. Data from respondents to survey 4, aged 28‐33 years (2006, n = 9,145) were analyzed. The main outcome measures were PCOS prevalence and body mass index (BMI).ResultsSelf‐reported PCOS prevalence was 5.8% (95% CI: 5.3%‐6.4%). Women reporting PCOS had higher weight, mean BMI [2.5 kg/m2 (95% CI: 1.9‐3.1)], and greater 10‐year weight gain [2.6 kg (95% CI: 1.2‐4.0)]. BMI was the strongest correlate of PCOS status with every BMI increment increasing the risk of reporting PCOS by 9.2% (95% CI: 6%‐12%).ConclusionsThis community based observational study with longitudinal reporting of weight shows that weight, BMI, and 10‐year weight gain were higher in PCOS. We report the novel finding that obesity and greater weight gain are significantly associated with PCOS status. Considering the prevalence, major health and economic burden of PCOS, the increasing weight gain in young women, and established benefits of weight loss, these results have major public health implications.
Metastable β‐Bi2O3 Nanoparticles with Potential for Photocatalytic Water Purification Using Visible Light Irradiation
Authors: Schlesinger Maik , Weber Marcus , Schulze Steffen , Hietschold Michael , Mehring Michael 
Published: July 2013

AbstractPhotocatalytic studies under visible light irradiation using nanosized β‐Bi2O3 are reported. β‐Bi2O3 nanoparticles are prepared starting from the well‐defined bismuth oxido cluster [Bi38O45(OMc)24(DMSO)9]⋅2 DMSO⋅7 H2O (OMc=O2CC3H5) using a straightforward hydrolysis and annealing protocol. Powder X‐ray diffraction studies, transmission electron microscopy, diffuse reflectance UV/Vis spectroscopy and nitrogen adsorption measurements (using the Brunauer–Emmett–Teller (BET) theory) are used for the characterization of the as‐prepared β‐Bi2O3. By time‐dependent annealing, the crystallite size can be controlled between (17±2) nm and (45±5) nm with BET surface areas of 7 to 29 m2 g−1. The indirect band gap of the as‐prepared β‐Bi2O3 amounts to (2.15±0.05) eV. The decomposition rates for rhodamine B (RhB) solutions are in the range of 2.46×10−5 to 4.01×10−4 s−1 and depend on the crystallite size, amount of catalyst and concentration of RhB. Photocorrosion experiments have shown the formation of Bi2O2CO3 after several catalytic cycles. However, the catalyst can be recycled to phase‐pure β‐Bi2O3 nanoparticles by annealing for one hour under argon atmosphere at 380 °C. Furthermore, the photocatalytic activity of as‐prepared β‐Bi2O3 nanoparticles for the decomposition of phenol, 4‐chlorophenol, 2,4‐dichlorphenol, 4‐nitrophenol, triclosan and ethinyl estradiol is demonstrated.
MFe2O4 and MFe@Oxide Core–Shell Nanoparticles Anchored on N‐Doped Graphene Sheets for Synergistically Enhancing Lithium Storage Performance and Electrocatalytic Activity for Oxygen Reduction Reactions
Authors: Xiao Junwu , Xu Guiliang , Sun Shi‐Gang , Yang Shihe 
Published: July 2013

The intrinsically low electric conductivity and self‐aggregation of MFe2O4 during charge/discharge affect their lithium storage performance and electrocatalytic activity. To mitigate these problems, it is shown that N‐doped graphene sheets (NGS), as a highly conductive platform, finely disperse the MFe2O4 nanoparticles and rapidly shuttle electrons to and from the MFe2O4 nanoparticles. Moreover, by forming a metal@oxide core–shell nanostructure, fast electron transfer from the exterior oxide layer to NGS is achieved. Introducing NGS into MFe2O4 allows the composites to exhibit the comparable specific capacity (based on the total mass) to MFe2O4, although over 10 wt% of NGS contributes a low specific capacity of around 320–400 mAh g−1. More importantly, introducing NGS significantly increases the cycling stability performance: 97.5% (CoFe2O4/NGS) and ≈100% (NiFe2O4/NGS) of the specific capacities have been retained after 80 cycles, far higher than the capacity retentions of CoFe2O4 (35.3%) and NiFe2O4 (43.7%) tested under otherwise identical conditions. Also demonstrated are the excellent rate capabilities of the composites. For catalyzing the oxygen reduction reaction, the activity is significantly improved when the MFe2O4 nanoparticles are transformed into metal@oxide core–shell nanostructure, mainly because the core–shell nanostructure exhibits lower charge transfer resistance.
Variable clinical outcome of ABCA3 deficiency in two siblings
Authors: Thavagnanam Surendran , Cutz Ernest , Manson David , Nogee Lawrence M. , Dell Sharon D. 
Published: July 2013

AbstractThis case report describes an unusual outcome of ABCA3 deficiency with resolution of symptoms, normalization of chest imaging and lung function in a 9‐year‐old child whose sibling died of the same disease in infancy. Pediatr Pulmonol. 2013; 48:1035–1038. © 2013 Wiley Periodicals, Inc.
Clinical and epidemiologic profile of lower respiratory tract infections associated with human bocavirus
Authors: do Amaral de Leon Cristiano , Amantea Sérgio Luis , Pilger Diogo André , Cantarelli Vlademir 
Published: July 2013

AbstractBackgroundAcute viral bronchiolitis (AVB) remains the leading cause of lower respiratory tract infection (LRTI) in infants under 2 years of age. Advances in molecular methods for virus detection have led to the identification of new infectious agents implicated in the development of AVB, such as human bocavirus (HBoV).ObjectivesTo ascertain the frequency, seasonality, and clinical behavior of HBoV detection in a series of episodes of LRTI.Study DesignThe frequency of HBoV was assessed in children with LRTI episodes, aged 1–24 months, seen at the emergency department of Hospital da Criança Santo Antônio, Porto Alegre, Brazil, between May 2007 and July 2008. Virus‐specific polymerase chain reaction was used for detection.ResultsA total of 455 nasal secretion samples were collected from 433 patients over a 14‐month period. Of these, 60 were positive for HBoV (13.2%). Mean age was 7.9 months and 55% of patients were male. Just over half of patients were under 6 months of age (53.3%). Wheezing was the presenting respiratory complaint in 51.7%. Of the 60 patients, 80% were admitted to a pediatric ward. Diarrhea was present in nine patients (18%). Co‐detection was a frequent finding in our sample, occurring in 95% of cases. In our series, the distribution of HBoV was clearly seasonal and was influenced by temperature, relative humidity, and precipitation.ConclusionsWe conclude that HBoV detection in infants with AVB and recurrent wheezing of viral etiology in Brazil is similar to that reported in other countries. The clinical course of HBoV detection is no different from that of other respiratory viruses commonly found in this age range. Pediatr Pulmonol. 2013; 48:1112–1118. © 2013 Wiley Periodicals, Inc.
Ionic strength‐dependent conformations of a ubiquitin‐like small archaeal modifier protein (SAMP1) fromHaloferax volcanii
Authors: Ye Kaiqin , Liao Shanhui , Zhang Wen , Fan Kai , Zhang Xuecheng , Zhang Jiahai , Xu Chao , Tu Xiaoming 
Published: July 2013

AbstractEukaryotic ubiquitin and ubiquitin‐like systems play crucial roles in various cellular biological processes. In this work, we determined the solution structure of SAMP1 fromHaloferax volcaniiby NMR spectroscopy. Under low ionic conditions, SAMP1 presented two distinct conformations, one folded β‐grasp and the other disordered. Interestingly, SAMP1 underwent a conformational conversion from disorder to order with ion concentration increasing, indicating that the ordered conformation is the functional form of SAMP1 under the physiological condition ofH. volcanii. Furthermore, SAMP1 could interact with proteasome‐activating nucleotidase B, supposing a potential role of SAMP1 in the protein degradation pathway mediated by proteasome.
Crystal structure of the ubiquitin‐like small archaeal modifier protein 2 from Haloferax volcanii
Authors: Li Yunfeng , Maciejewski Mark W. , Martin Jonathan , Jin Kai , Zhang Yuhang , Maupin‐Furlow Julie A. , Hao Bing 
Published: July 2013

AbstractThe discovery of ubiquitin‐like small archaeal modifier protein 2 (SAMP2) that forms covalent polymeric chains in Haloferax volcanii has generated tremendous interest in the function and regulation of this protein. At present, it remains unclear whether the Hfx. volcanii modifier protein SAMP1 has such polyubiquitinating‐like activity. Although SAMP1 and SAMP2 use the same conjugation machinery to modify their target proteins, each can impart distinct functional consequences. To better understand the mechanism of SAMP2 conjugation, we have sought to characterize the biophysical and structural properties of the protein from Hfx. volcanii. SAMP2 is only partially structured under mesohalic solution conditions and adopts a well‐folded compact conformation in the presence of 2.5M of NaCl. Its 2.3‐Å‐resolution crystal structure reveals a characteristic α/β central core domain and a unique β‐hinge motif. This motif anchors an unusual C‐terminal extension comprising the diglycine tail as well as two lysine residues that can potentially serve to interlink SAMP2 moieties. Mutational alternation of the structural malleability of this β‐hinge motif essentially abolishes the conjugation activity of SAMP2 in vivo. In addition, NMR structural studies of the putative ubiquitin‐like protein HVO_2177 from Hfx. volcanii show that like SAMP1, HVO_2177 forms a classic β‐grasp fold in a salt‐independent manner. These results provide insights into the structure–function relationship of sampylating proteins of fundamental importance in post‐translational protein modification and environmental cues in Archaea.
The role of carbon dioxide as an orientation cue for western corn rootworm larvae within the maize root system: implications for an attract‐and‐kill approach
Authors: Schumann Mario , Patel Anant , Vemmer Marina , Vidal Stefan 
Published: July 2013

AbstractBACKGROUNDWestern corn rootworm larvae use CO2 to locate maize roots. However, the importance of CO2 as a specific orientation cue close to maize roots has not been investigated unequivocally. This study aimed at elucidating the effect of CO2‐emitting capsules in combination with a soil insecticide (Tefluthrin = attract and kill) within the root system. We hypothesized that the capsules would result in aggregation of the larvae at the soil insecticide, thus increasing its efficacy. A nondestructive observation device was used to study larval distribution and behaviour.RESULTSSpatial analysis of distance indices (SADIE) revealed aggregation of the larvae around the capsules in an attract‐and‐kill treatment after 4 h, which was not found with the conventional treatment without the capsules. However, larval mortality did not differ between treatments.CONCLUSIONCO2 is a weak attractant for western corn rootworm larvae within the root system. Consequently, an attract‐and‐kill strategy based on a CO2 product will not contribute to better control compared with conventional Tefluthrin applications. Host‐specific compounds, combined with a CO2 source, should be used to target more larvae, making attract and kill a feasible management option against this pest. © 2013 Society of Chemical Industry