bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biosynthesis of cupric oxide nanoparticles: its antiviral activities against TMV by directly destroying virion and inducing plant resistance
Authors: Liu Shuaikang , Tian Weiqiang , Liu Zhongwei , Wei Xuefeng , Yuan Kai , Du Wei , Chen Siang , Chen Shuhan , Zhou Dong , Cai Lin 
Published: June 2024

AbstractTobacco mosaic virus (TMV) is widely recognized as one of the most important plant viruses, causing significant agricultural losses in terms of both quality and yield worldwide each year. This study demonstrated the biosynthesis of copper oxide nanoparticles (CuONPs) using orange peel extract for effective control of TMV infection both in vitro and in vivo. After treatment with CuONPs (100 mg/L) for 2 h, TMV particles exhibited evident fragmentation in vitro, reducing infectivity on tobacco plants. Similarly, the application of CuONPs on Nicotiana benthamiana (N. benthamiana) positively impeded viral replication and accumulation in vivo. Interestingly, the expression of systemic resistance-related genes (PR1, PR2, ERF1, and JAZ3) in the host plant was up-regulated by CuONPs treatment, supporting that CuONPs activated plant immunity to inhibit TMV. Importantly, the application of CuONPs (100 mg/L) did not exhibit any toxic effects on tobacco and, instead, resulted in the promotion of chlorophyll content, as well as an increase in the fresh weight and dry weight of the plant when compared to the control treatment. Overall, we proposed that the appropriate concentration of CuONPs (100 mg/L) can directly break viral particles by passivating, boost plant immunity by stimulating systemic acquired resistance (SAR), and provide nutritional supplements to promote plant growth.
Gut microbiota and immune profiling of microbiota-humanised versus wildtype mouse models of hepatointestinal schistosomiasis
Authors: Stark K. A. , Rinaldi G. , Costain A. , Clare S. , Tolley C. , Almeida A. , McCarthy C. , Harcourt K. , Brandt C. , Lawley T. D. , Berriman M. , MacDonald A. S. , Forde-Thomas J. E. , Hulme B. J. , Hoffmann K. F. , Cantacessi C. , Cortés A. 
Published: June 2024

AbstractMounting evidence of the occurrence of direct and indirect interactions between the human blood fluke, Schistosoma mansoni, and the gut microbiota of rodent models raises questions on the potential role(s) of the latter in the pathophysiology of hepatointestinal schistosomiasis. However, substantial differences in both the composition and function between the gut microbiota of laboratory rodents and that of humans hinders an in-depth understanding of the significance of such interactions for human schistosomiasis. Taking advantage of the availability of a human microbiota-associated mouse model (HMA), we have previously highlighted differences in infection-associated changes in gut microbiota composition between HMA and wildtype (WT) mice. To further explore the dynamics of schistosome-microbiota relationships in HMA mice, in this study we (i) characterize qualitative and quantitative changes in gut microbiota composition of a distinct line of HMA mice (D2 HMA) infected with S. mansoni prior to and following the onset of parasite egg production; (ii) profile local and systemic immune responses against the parasite in HMA as well as WT mice and (iii) assess levels of faecal inflammatory markers and occult blood as indirect measures of gut tissue damage. We show that patent S. mansoni infection is associated with reduced bacterial alpha diversity in the gut of D2 HMA mice, alongside expansion of hydrogen sulphide-producing bacteria. Similar systemic humoral responses against S. mansoni in WT and D2 HMA mice, as well as levels of faecal lipocalin and markers of alternatively activated macrophages, suggest that these are independent of baseline gut microbiota composition. Qualitative comparative analyses between faecal microbial profiles of S. mansoni-infected WT and distinct lines of HMA mice reveal that, while infection-induced alterations of the gut microbiota composition are highly dependent on the baseline flora, bile acid composition and metabolism may represent key elements of schistosome-microbiota interactions through the gut-liver axis.
Synergizing MBR and MCDI systems as a sustainable solution for decentralized wastewater reclamation and reuse
Authors: Yu Wang-Sheng , Liu Huei-Cih , Lin Hsin-Chieh , Lee Mengshan , Hou Chia-Hung 
Published: June 2024

AbstractDecentralized wastewater reclamation and reuse systems have drawn much attention due to their capability for reducing the energy demand for water conveyance and reclaiming wastewater for local re-use. While membrane bioreactor (MBR) stands as a mature technology offering comprehensive solid and liquid separation, membrane capacitive deionization (MCDI) presents a promising avenue for ion separation. Unfortunately, MCDI has seldom been incorporated into decentralized wastewater reclamation and reuse systems. This study aims to exemplify the design and the operation of the synergistic integration of MBR and MCDI system with a practical capacity of 1 m3 d− 1, showcasing its efficacy in reclaiming and reusing water at regional level. The integrated system demonstrated significant high removal of total organic carbon (from 97 to 2 mg L− 1) and chemical oxygen demand (COD, from 218 to < 3 mg L− 1). Meantime, nearly complete transformation (approximately 91%) of NH3 to NO3− within the MBR effluent was observed with a hydraulic retention time of 4.3–4.8 h and a food-to-microorganism of 0.15–0.20 kg COD kg− 1 MLSS d− 1 which can be further removed through the MCDI system (> 92% TN removal). A significant milestone of MCDI unit was reached with the remarkable removal efficiency of total ions (93%) and water recovery (80%) using a stop-flow regeneration approach coupled with an optimized voltage of 2.0 V. The MCDI unit not only proved its high stability but also featured low energy consumption (0.44 kWh m− 3). Overall, synergizing MBR and MCDI systems emerges as a sustainable and effective solution for decentralized wastewater reclamation and reuse, contributing to a more environmentally friendly and resource-efficient water management paradigm.
Impact of biopesticides on the probing and feeding behavior of
Aphis gossypii
Authors: Hamouche Zeinab , Zippari Caterina , Boucherf Abderrahmane , Cavallo Giuseppe , Djelouah Khaled , Tamburini Giovanni , Verrastro Vincenzo , Biondi Antonio , Cornara Daniele 
Published: June 2024

Abstract

Background
Pesticides-induced mortality has traditionally been the toxicological endpoint on which a chemical is selected for pest management strategies. However, the exposure to a pesticide might also cause behavioral alterations that can dramatically affect the dynamics of pest-plant interaction. Understanding these non-lethal effects is crucial for developing comprehensive and sustainable pest control measures.

Methods

Here, using the Electrical Penetration Graph (EPG) technique, we evaluated whether biopesticides routinely used in organic or conventional pest management might alter the probing and feeding behavior of the cotton aphid
Aphis gossypii
on treated plants. The post exposure persistence of feeding alterations when moving the insect onto clean plants was also assessed. The tested biopesticides were chosen for being aphicides or because used in those crops hosts of
A. gossypii
. Generalised linear mixed models were fitted to analyse the effects of biopesticides on the probing and feeding behavior of aphids, using untreated control data as a model baseline.

Results

Residuals of
Bacillus thuringiensis
,
Beauveria bassiana
, and sulfur significantly affected the dynamics of the stylets intercellular route compared to the control (namely, shorter pathways, fewer and shorter brief intracellular stylets probes, higher and longer derailed stylets events). Mineral oil, orange essential oil and pyrethrin delayed or impaired the onset of phloem sap ingestion. On the other hand, copper and potassium salts promoted feeding. Pyrethrin was the only biopesticides inducing persistent behavioral alterations, with insects displaying a higher frequency of occurrence of xylem ingestion events when moved to untreated plants after exposure.

Conclusions
Overall, this study demonstrates that biopesticides modulate the aphid-plant interactions by impairing or facilitating the exploitation of the host plant, also affecting the patterns conducive to plant pathogens transmission. This study also highlights the importance of considering in toxicological studies the impact of all the compounds the pest could be exposed to within the agroecosystem, including those not designed for that specific pest.
Overexpression of EspL inhibits autophagy and antigen presentation to promote the intracellular survival of Mycobacterium tuberculosis avirulent strains
Authors: Cai Luxia , Lei Yingying , Xie Tianyi , Liu Yiling , Fan Yutong , Yang Bing , Dong Shuang , Cao Gang , Chen Xi 
Published: June 2024

AbstractMycobacterium tuberculosis (Mtb) employs multiple mechanisms, such as phagocytosis and autophagy, to evade innate immune clearance and establish infection. In the present study, we identified the ESX-1 secretion-associated protein EspL, which promotes Mtb survival by inhibiting phagosome maturation and autophagy initiation. EspL knockout decreased Mtb intracellular survival, while EspL overexpression increased bacterial survival by interfering with phagocytosis and autophagy. EspL interacts with ULK1 and promotes its phosphorylation at Ser757, leading to the inhibition of autophagy initiation. Additionally, overexpression of EspL reduced antigen presentation and T-cell responses both in vitro and in vivo. Our findings revealed that EspL interferes with autophagy and antigen presentation by suppressing ULK1 activation. These insights provide a novel understanding of Mtb pathogenicity.
Bynum, Gender, and the Western Christian Middle Ages
Authors: Harrison Anna 
Published: June 2024

Abstract
As a contribution to the Common Knowledge symposium “Caroline Walker Bynum across the Disciplines,” this article argues that Bynum's work on gender has overturned bedrock interpretations of the religious significance of the widespread ascetic practices of the Western Christian Middle Ages. Bynum's claim has been that medieval asceticism is best understood not as an upshot of dualism — of the soul and body understood as in opposition — but as “an effort to plumb and realize all the possibilities of the flesh.” Drawing on underused religious sources, analyzing in their larger social and cultural context the images that medieval women and men used, and employing a comparative analysis of female and male spiritualities, she has demonstrated that each had its own different emphases. In this effort, she has pioneered what is sometimes called a “history of masculinities.” Focusing on women's own experience and ferreting out basic assumptions of their religious thought and practice, she has argued for women's profound contributions to their contemporary theological culture by drawing on the dominant culture's hierarchical gender binaries in order to undermine them and accentuate their shared (bodily) humanity with Christ. Bynum has shown successfully that medieval women had confidence in their own ability to love and imitate Christ and thus to participate in the salvation of the world.
Caroline Bynum and Medieval Art History in America
Authors: Jung Jacqueline E. 
Published: June 2024

Abstract
As a contribution to the Common Knowledge symposium “Caroline Walker Bynum across the Disciplines,” this essay stresses Bynum's commitment to the methods and questions of history but also the unparalleled impact of her work on adjacent fields, including and perhaps even especially art history. Furthermore, her body of scholarship registers a consistent engagement with art historians. Weaving together personal memoir and historiography, this article sketches the manifold ways in which Bynum's publications have responded to and shaped the contours of medieval art history in America since the early 1990s — particularly with respect to questions of gender, body, identity, violence, and materiality — and the formative role that her mentorship and pedagogy have played in the author's development as an art historian.
Full-Endoscopic Sacroiliac Joint Denervation for Painful Sacroiliac Joint Dysfunction: A Prospective 2-Year Clinical Outcomes and Predictors for Improved Outcomes
Authors: Hasan Saqib , Halalmeh Dia Radi , Ansari Yusuf-Zain , Herrera Amy , Hofstetter Christoph P. 
Published: June 2024

BACKGROUND AND OBJECTIVES:
Full-endoscopic sacroiliac joint denervation (FE-SJD) is a novel technique for the management of pain secondary to sacroiliac joint dysfunction. The aim of this study was to assess the long-term efficacy, safety, clinical outcomes, and outcome predictors of uniportal full-endoscopic sacroiliac joint denervation.

METHODS:
From 2019 to 2021, a total of 47 consecutive patients with pain secondary to sacroiliac joint dysfunction underwent uniportal FE-SJD through posterior approach by a single fellowship-trained spine surgeon. A retrospective analysis of perioperative parameters, complications, and clinical outcomes were obtained prospectively.

RESULTS:
The patient cohort had a mean age of 59.4 ± 14.0 years, with 63.8% females. Symptom duration averaged 62.1 ± 53.7 months. The mean operative time was 57.1 ± 16.8 minutes. All patients were discharged on the same day of surgery. Significant improvement was noted in preoperative visual analog score (back) and Oswestry Disability Index scores at 3, 6, 12 months, and 2 years (P < .001). Thirty-four patients (72.3%) returned to normal functioning with an average of 82% pain relief and a satisfaction rate of 78.7% at a mean follow-up of 18.2 ± 13.1 months. There were no intraoperative complications. One patient had postoperative right L5 dysesthesia. Seven patients (14.9%) underwent contralateral FE-SJD due to satisfaction with the index procedure but residual pain on the contralateral side. Concomitant lumbar issues correlated with less functional improvement at 2 years (P = .009).

CONCLUSION:
The long-term clinical results of FE-SJD are favorable. Endoscopic denervation of the dorsal rami branches supplying the sacroiliac joint represents a safe, effective, and durable option to address pain secondary to sacroiliac joint dysfunction. A significant factor that influences outcomes is the presence of concomitant lumbar pathology. Further research is needed to compare this technique with current available treatment options.
The Harawayan Bee Hotel: a tool to catalyse emancipatory change within and beyond the Education for Sustainable Development agenda in pre-service teacher training
Authors: Bernardini Marco , Reed Johnson Jo Anna 
Published: June 2024

The notion of the Anthropocene has become a popular (and contested) term to describe the times we live in; among other things, it alerts us to the damage mainstream Western-centred anthropocentrism has wreaked on nature: in so doing, the Anthropocene signals that for life as we know it to continue, a more sustainable relationship with nature must be urgently implemented.
The article will discuss a project that emerged as part of a teacher education programme in the UK where selected insights elaborated by Donna Haraway have been used to inform a Bee Hotel project. The resulting ‘Harawayan’ Bee Hotel (HBH) was used as a catalyst to help trainee teachers to both blend climate education into the standard curriculum to be delivered during their placements and, importantly, to introduce them to a new conceptualisation of nature. Specifically, trainee teachers were presented with, and encouraged to integrate into their teaching practices, a vision of nature that recognises and respects its uniqueness, agency and worth, and that accepts that some level of ecological instrumentalisation and destruction is necessary for human life.
The article will argue that the HBH acts as a microcosm where it is possible to forge and practice, for both present and future generations, an ethics that encourages the establishment of a respectful relationship with nature, facilitating the meeting of SDGs and offering the thinking tools to go beyond them.
Differences in the consolidation by spontaneous and evoked ripples in the presence of active dendrites
Authors: Jauch Jannik , Becker Moritz , Tetzlaff Christian , Fauth Michael Jan 
Published: June 2024

Ripples are a typical form of neural activity in hippocampal neural networks associated with the replay of episodic memories during sleep as well as sleep-related plasticity and memory consolidation. The emergence of ripples has been observed both dependent as well as independent of input from other brain areas and often coincides with dendritic spikes. Yet, it is unclear how input-evoked and spontaneous ripples as well as dendritic excitability affect plasticity and consolidation.
Here, we use mathematical modeling to compare these cases. We find that consolidation as well as the emergence of spontaneous ripples depends on a reliable propagation of activity in feed-forward structures which constitute memory representations. This propagation is facilitated by excitable dendrites, which entail that a few strong synapses are sufficient to trigger neuronal firing. In this situation, stimulation-evoked ripples lead to the potentiation of weak synapses within the feed-forward structure and, thus, to a consolidation of a more general sequence memory. However, spontaneous ripples that occur without stimulation, only consolidate a sparse backbone of the existing strong feed-forward structure.
Based on this, we test a recently hypothesized scenario in which the excitability of dendrites is transiently enhanced after learning, and show that such a transient increase can strengthen, restructure and consolidate even weak hippocampal memories, which would be forgotten otherwise. Hence, a transient increase in dendritic excitability would indeed provide a mechanism for stabilizing memories.
Addressing a missed opportunity for contraceptive use during the extended postpartum period by integrating it with infant immunization services in Sidama Region, Ethiopia: A quasi-experimental study
Authors: Muluneh Abebaw Abeje , Gedefaw Abel , Kelaye Temesgen , Sisay Friehiwot , Worku Misganaw , Astatkie Ayalew , Shiferaw Solomon 
Published: June 2024

Globally, unmet need for postpartum family planning is high. However, immunization services are among the most widely utilized health services. Establishing systematic screening, counseling, and referral systems from different contact points, particularly from EPI units may improve postpartum family planning uptake. Hence, this study aimed to assess the effect of counseling for family planning at EPI units on contraceptive uptake during the extended post-partum period. A before-and-after type of quasi-experimental study was conducted in 8 purposively selected primary health care units in Sidama region, Ethiopia. All mothers visiting the selected health facilities for infant immunization services from February 06 to August 30, 2020, were screened, counseled, and referred for family planning. A structured interviewer-administered questionnaire was used to collect data from 1421 randomly selected mothers (717 for pre-intervention and 704 post-intervention phases). EpiData version 3.1 and SPSS version 22 were used for data entry and analysis. The effect of the intervention was assessed using multivariable logistic regression analysis adjusting for the effects of potential confounders. P value < 0.05 was considered statistically significant. The contraceptive utilization rate before intervention was 72.7% with 95% CI (69.5, 75.9). It was 91.9%, 95% CI (89.8%, 93.9%) after the intervention. Utilization of contraceptive pills increased from 4.3% to 6.9%, injectables from 52.4% to 57.5%, implants from 12.8% to 22.9%, and IUCD from 3.2% to 5.0% after the intervention. After adjusting for the effect of possible confounding variables, screening, counseling, and referring mothers for family planning at infant immunization units significantly increases the contraceptive utilization rate among mothers presented for infant immunization services(AOR = 5.83, 95% CI: 4.02, 8.46). Screening, counseling, and referring mothers for family planning services at infant immunization units significantly increases postpartum contraceptive uptake. Integrating family planning messages with infant immunization services is recommended.
Trial registration: ClinicalTrials.gov Identifier: NCT04767139 (Registered on 23/02/2021).
Frequency of breaks, amount of muscular rest, and sustained muscle activity related to neck pain in a pooled dataset
Authors: Koch Markus , Forsman Mikael , Enquist Henrik , Baare Olsen Henrik , Søgaard Karen , Sjøgaard Gisela , Østensvik Tove , Nilsen Petter , Andersen Lars Louis , Due Jacobsen Markus , Brandt Mikkel , Westgaard Rolf , Mork Paul Jarle , Fan Xuelong , Wærsted Morten , Veiersted Kaj Bo 
Published: June 2024

Background
Neck pain remains a persistent challenge in modern society and is frequently encountered across a wide range of occupations, particularly those involving repetitive and monotonous tasks. It might be expected that patterns of trapezius muscle activity at work, characterized by few breaks and prolonged periods of sustained muscle activity, are linked to neck pain. However, previous cross-sectional studies have generally failed to establish a definitive association. While some longitudinal studies have suggested that extended periods of heightened muscle activity could be a risk factor for neck pain, these findings often relied on limited participant numbers or specific professional groups. This study aimed to investigate the relationship between trapezius muscle activity and neck pain by pooling data from seven Scandinavian research institutes encompassing a diverse range of occupational backgrounds.

Methods
Electromyographic (EMG) data for the upper trapezius muscle, collected during working hours, were coupled with questionnaire responses pertaining to neck pain, individual characteristics, and potential confounding variables for a total of 731 subjects. Additionally, longitudinal data from 258 subjects were available. The various EMG datasets were consolidated into a standardized format, and efforts were made to harmonize inquiries about neck pain. Regression analyses, adjusting for sex and height, were conducted to explore the associations between muscle activity variables and neck pain. An exposure index was devised to quantify the cumulative neck load experienced during working hours and to differentiate between various occupational categories.

Results
The cross-sectional data displayed a distinct pattern characterized by positive associations for brief periods of sustained muscle activity (SUMA) and negative associations for prolonged SUMA-periods and neck pain. The longitudinal data exhibited a contrasting trend, although it was not as pronounced as the cross-sectional findings. When employing the exposure index, notable differences in cumulative muscle load emerged among occupational groups, and positive associations with longitudinal neck pain were identified.

Discussion
The results suggest that individuals with neck pain experience higher cumulative workloads and extended periods of muscle activity over the long term. In the short term, they appear to compensate by taking frequent short breaks, resulting in a lower cumulative workload. Regardless of their occupation, it is crucial to distribute work breaks throughout the workday to ensure that the cumulative load remains manageable.