bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced tolerance of transgenic potato plants expressing choline oxidase in chloroplasts against water stress
Authors: Cheng Yu-Jie , Deng Xi-Ping , Kwak Sang-Soo , Chen Wei , Eneji Anthony E 
Published: September 2013

Abstract

Background
Glycinebetaine, whose biosynthesis could be catalyzed by choline oxidase (COD), is an extremely efficient compatible solute for scavenging oxidative stress-inducing molecules and protecting the photosynthetic system in plants. To study the effects of the codA transgene for choline oxidase on the drought resistance and recovery, a transgenic potato cultivar (SC) bearing codA gene and a non-transgenic (NT) control cultivar were raised in pots under moderate and severe drought stress. The experiment was constituted by a two-day-pretreatment with 20% PEG and a four-day-water stress combined with two-day-recovery treatment.

Results
Under the four-day-water stress, plants were provided with normal water condition, 10% or 20% polyethylene glycol. The results of pretreatment showed an expression of codA gene in transgenic potato and an accumulation of glycine betaine (GB); leaf water potential was higher in SC than in NT. In the stress-recovery-treatment, SC showed stronger antioxidant ability, more efficient photosynthetic system, higher chlorophyll content, lower malondialdehyde content and better recovery from water deficit stress than NT.

Conclusion
Although this work concentrated on the short-term water stress and recover treatments on transgenic potato plants with the over-expression of CodA gene and its control line. The datas shows that the exogenous codA gene provided potato a stronger drought resistance and recovery ability.
Comparative testicular transcriptome of wild type and globozoospermic Dpy19l2knock out mice
Authors: Karaouzène Thomas , El Atifi Michèle , Issartel Jean-Paul , Grepillat Marianne , Coutton Charles , Martinez Delphine , Arnoult Christophe , Ray Pierre F 
Published: September 2013

Abstract

Background
Globozoospermia is a male infertility phenotype characterized by the presence in the ejaculate of near 100% acrosomeless round-headed spermatozoa with normal chromosomal content. Following intracytoplasmic sperm injection (ICSI) these spermatozoa give a poor fertilization rate and embryonic development. We showed previously that most patients have a 200 kb homozygous deletion, which includes DPY19L2 whole coding sequence. Furthermore we showed that the DPY19L2 protein is located in the inner nuclear membrane of spermatids during spermiogenesis and that it is necessary to anchor the acrosome to the nucleus thus performing a function similar to that realized by Sun proteins within the LINC-complex (Linker of Nucleoskeleton and Cytoskeleton). SUN1 was described to be necessary for gametogenesis and was shown to interact with the telomeres. It is therefore possible that Dpy19l2 could also interact, directly or indirectly, with the DNA and modulate gene expression during spermatogenesis.
In this study, we compared the transcriptome of testes from Dpy19l2 knock out and wild type mice in order to identify a potential deregulation of transcripts that could explain the poor fertilization potential of Dpy19l2 mutated spermatozoa.

Methods
RNA was extracted from testes from DPY19L2 knock out and wild type mice. The transcriptome was carried out using GeneChip® Mouse Exon 1.0 ST Arrays. The biological processes and molecular functions of the differentially regulated genes were analyzed with the PANTHER software.

Results
A total of 76 genes were deregulated, 70 were up-regulated and 6 (including Dpy19l2) were down-regulated. These genes were found to be involved in DNA/RNA binding, structural organization, transport and catalytic activity.

Conclusions
We describe that an important number of genes are differentially expressed in Dpy19l2 mice. This work could help improving our understanding of Dpy19l2 functions and lead to a better comprehension of the molecular mechanism involved in spermatogenesis.
The systemic iron-regulatory proteins hepcidin and ferroportin are reduced in the brain in Alzheimer’s disease
Authors: Raha Animesh Alexander , Vaishnav Radhika Anand , Friedland Robert Paul , Bomford Adrian , Raha-Chowdhury Ruma 
Published: September 2013

Abstract

Background
The pathological features of the common neurodegenerative conditions, Alzheimer’s disease (AD), Parkinson’s disease and multiple sclerosis are all known to be associated with iron dysregulation in regions of the brain where the specific pathology is most highly expressed. Iron accumulates in cortical plaques and neurofibrillary tangles in AD where it participates in redox cycling and causes oxidative damage to neurons. To understand these abnormalities in the distribution of iron the expression of proteins that maintain systemic iron balance was investigated in human AD brains and in the APP-transgenic (APP-tg) mouse.

Results
Protein levels of hepcidin, the iron-homeostatic peptide, and ferroportin, the iron exporter, were significantly reduced in hippocampal lysates from AD brains. By histochemistry, hepcidin and ferroportin were widely distributed in the normal human brain and co-localised in neurons and astrocytes suggesting a role in regulating iron release. In AD brains, hepcidin expression was reduced and restricted to the neuropil, blood vessels and damaged neurons. In the APP-tg mouse immunoreactivity for ferritin light-chain, the iron storage isoform, was initially distributed throughout the brain and as the disease progressed accumulated in the core of amyloid plaques. In human and mouse tissues, extensive AD pathology with amyloid plaques and severe vascular damage with loss of pericytes and endothelial disruption was seen. In AD brains, hepcidin and ferroportin were associated with haem-positive granular deposits in the region of damaged blood vessels.

Conclusion
Our results suggest that the reduction in ferroportin levels are likely associated with cerebral ischaemia, inflammation, the loss of neurons due to the well-characterised protein misfolding, senile plaque formation and possibly the ageing process itself. The reasons for the reduction in hepcidin levels are less clear but future investigation could examine circulating levels of the peptide in AD and a possible reduction in the passage of hepcidin across damaged vascular endothelium. Imbalance in the levels and distribution of ferritin light-chain further indicate a failure to utilize and release iron by damaged and degenerating neurons.
Combination of extracts from Aristolochia cymbifera with streptomycin as a potential antibacterial drug
Authors: Silva Willer F , Cecílio Samyra G , Magalhães Cintia LB , Ferreira Jaqueline MS , Tótola Antonio H , de Magalhaes Jose C 
Published: September 2013

Abstract
The appearance of new antibiotic-resistant bacteria is a societal problem that requires the development of new alternative treatments. Therefore, this work evaluated the antibacterial activity of ethanolic (EHI), dichloromethanic (EDI) and hexanic (EHE) extracts from Aristolochia cymbifera stems and the combination of these extracts with an antimicrobial drug to develop a new antibacterial therapy. The EDI, EHE and EHI extracts were obtained by maceration using three different solvents. The minimal inhibitory concentrations (MIC) of these extracts were determined using the microdilution test to determine the antibacterial potential of these extracts and their combination with streptomycin against Staphylococcus aureus, Bacillus cereus, Klebsiella pneumoniae and Shigella flexneri. The extract dose leading to the cytotoxicity of 50% of the cells (CC50) was evaluated using mammalian cells MA104 and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay. The extracts had a MIC under 500 mg/L and a CC50 lower than 50 mg/L. The antibiotic/extract proportion influenced the antibacterial activity of the mixtures, and the proportion that optimized the antibacterial activity of streptomycin was a mixture that contained 75 percent of extract. This composition included less than 6.5 mg/L of extract and 2.5 mg/L of streptomycin and has potential as a new antibacterial therapy.
The microbiological and clinical outcome of guide wire exchanged versus newly inserted antimicrobial surface treated central venous catheters
Authors: Parbat Nisha , Sherry Norelle , Bellomo Rinaldo , Schneider Antoine G , Glassford Neil J , Johnson Paul DR , Bailey Michael 
Published: September 2013

Abstract
Introduction
The management of suspected central venous catheter (CVC)-related sepsis by guide wire exchange (GWX) is not recommended. However, GWX for new antimicrobial surface treated (AST) triple lumen CVCs has never been studied. We aimed to compare the microbiological outcome of triple lumen AST CVCs inserted by GWX (GWX-CVCs) with newly inserted triple lumen AST CVCs (NI-CVCs).

Methods
We studied a cohort of 145 consecutive patients with GWX-CVCs and contemporaneous site-matched control cohort of 163 patients with NI-CVCs in a tertiary intensive care unit (ICU).

Results
GWX-CVC and NI-CVC patients were similar for mean age (58.7 vs. 62.2 years), gender (88 (60.7%) vs. 98 (60.5%) male) and illness severity on admission (mean Acute Physiology and Chronic Health Evaluation (APACHE) III: 71.3 vs. 72.2). However, GWX patients had longer median ICU lengths of stay (12.2 vs. 4.4 days; P < 0.001) and median hospital lengths of stay (30.7 vs. 18.0 days; P < 0.001). There was no significant difference with regard to the number of CVC tips with bacterial or fungal pathogen colonization among GWX-CVCs vs. NI-CVCs (5 (2.5%) vs. 6 (7.4%); P = 0.90). Catheter-associated blood stream infection (CA-BSI) occurred in 2 (1.4%) GWX patients compared with 3 (1.8%) NI-CVC patients (P = 0.75). There was no significant difference in hospital mortality (35 (24.1%) vs. 48 (29.4%); P = 0.29).

Conclusions
GWX-CVCs and NI-CVCs had similar rates of tip colonization at removal, CA-BSI and mortality. If the CVC removed by GWX is colonized, a new CVC must then be inserted at another site. In selected ICU patients at higher central vein puncture risk receiving AST CVCs GWX may be an acceptable initial approach to line insertion.
Is non-operative management safe and effective for all splenic blunt trauma? A systematic review
Authors: Cirocchi Roberto , Boselli Carlo , Corsi Alessia , Farinella Eriberto , Listorti Chiara , Trastulli Stefano , Renzi Claudio , Desiderio Jacopo , Santoro Alberto , Cagini Lucio , Parisi Amilcare , Redler Adriano , Noya Giuseppe , Fingerhut Abe 
Published: September 2013

Abstract
Introduction
The goal of non-operative management (NOM) for blunt splenic trauma (BST) is to preserve the spleen. The advantages of NOM for minor splenic trauma have been extensively reported, whereas its value for the more severe splenic injuries is still debated. The aim of this systematic review was to evaluate the available published evidence on NOM in patients with splenic trauma and to compare it with the operative management (OM) in terms of mortality, morbidity and duration of hospital stay.

Methods
For this systematic review we followed the "Preferred Reporting Items for Systematic Reviews and Meta-analyses" statement. A systematic search was performed on PubMed for studies published from January 2000 to December 2011, without language restrictions, which compared NOM vs. OM for splenic trauma injuries and which at least 10 patients with BST.

Results
We identified 21 non randomized studies: 1 Clinical Controlled Trial and 20 retrospective cohort studies analyzing a total of 16,940 patients with BST. NOM represents the gold standard treatment for minor splenic trauma and is associated with decreased mortality in severe splenic trauma (4.78% vs. 13.5% in NOM and OM, respectively), according to the literature. Of note, in BST treated operatively, concurrent injuries accounted for the higher mortality. In addition, it was not possible to determine post-treatment morbidity in major splenic trauma. The definition of hemodynamic stability varied greatly in the literature depending on the surgeon and the trauma team, representing a further bias. Moreover, data on the remaining analyzed outcomes (hospital stay, number of blood transfusions, abdominal abscesses, overwhelming post-splenectomy infection) were not reported in all included studies or were not comparable, precluding the possibility to perform a meaningful cumulative analysis and comparison.

Conclusions
NOM of BST, preserving the spleen, is the treatment of choice for the American Association for the Surgery of Trauma grades I and II. Conclusions are more difficult to outline for higher grades of splenic injury, because of the substantial heterogeneity of expertise among different hospitals, and potentially inappropriate comparison groups.
Effects of prehospital epinephrine during out-of-hospital cardiac arrest with initial non-shockable rhythm: an observational cohort study
Authors: Goto Yoshikazu , Maeda Tetsuo , Goto Yumiko Nakatsu 
Published: September 2013

Abstract
Introduction
Few clinical trials have provided evidence that epinephrine administration after out-of-hospital cardiac arrest (OHCA) improves long-term survival. Here we determined whether prehospital epinephrine administration would improve 1-month survival in OHCA patients.

Methods
We analyzed the data of 209,577 OHCA patients; the data were prospectively collected in a nationwide Utstein-style Japanese database between 2009 and 2010. Patients were divided into the initial shockable rhythm (n = 15,492) and initial non-shockable rhythm (n = 194,085) cohorts. The endpoints were prehospital return of spontaneous circulation (ROSC), 1-month survival, and 1-month favorable neurological outcomes (cerebral performance category scale, category 1 or 2) after OHCA. We defined epinephrine administration time as the time from the start of cardiopulmonary resuscitation (CPR) by emergency medical services personnel to the first epinephrine administration.

Results
In the initial shockable rhythm cohort, the ratios of prehospital ROSC, 1-month survival, and 1-month favorable neurological outcomes in the non-epinephrine group were significantly higher than those in the epinephrine group (27.7% vs. 22.8%, 27.0% vs. 15.4%, and 18.6% vs. 7.0%, respectively; all P < 0.001). However, in the initial non-shockable rhythm cohort, the ratios of prehospital ROSC and 1-month survival in the epinephrine group were significantly higher than those in the non-epinephrine group (18.7% vs. 3.0% and 3.9% vs. 2.2%, respectively; all P < 0.001) and there was no significant difference between the epinephrine and non-epinephrine groups for 1-month favorable neurological outcomes (P = 0.62). Prehospital epinephrine administration for OHCA patients with initial non-shockable rhythms was independently associated with prehospital ROSC (adjusted odds ratio [aOR], 8.83, 6.18, 4.32; 95% confidence interval [CI], 8.01-9.73, 5.82-6.56, 3.98-4.69; for epinephrine administration times ≤9 min, 10-19 min, and ≥20 min, respectively), with improved 1-month survival when epinephrine administration time was <20 min (aOR, 1.78, 1.29; 95% CI, 1.50-2.10, 1.17-1.43; for epinephrine administration times ≤9 min and 10-19 min, respectively), and with deteriorated 1-month favorable neurological outcomes (aOR, 0.63, 0.49; 95% CI, 0.48-0.80, 0.32-0.71; for epinephrine administration times 10-19 min and ≥20 min, respectively).

Conclusions
Prehospital epinephrine administration for OHCA patients with initial nonshockable rhythms was independently associated with achievement of prehospital ROSC and had association with improved 1-month survival when epinephrine administration time was <20 min.
Multifrequency full-waveform sonic logging in the screened interval of a large-diameter production well
Authors: Almalki Majed , Harris Brett , Dupuis J. Christian 
Published: September 2013

A set of field experiments using multiple transmitter center frequencies was completed to test the application potential of low-frequency full-waveform sonic logging in large-diameter production wells. Wireline logs were acquired in a simple open drillhole and a high-yield large diameter production well completed with wire-wound sand screens at an aquifer storage and recovery site in Perth, Western Australia. Phase-shift transform methods were applied to obtain phase-velocity dispersion images for frequencies of up to 4 kHz. A 3D representation of phase-velocity dispersion was developed to assist in the analysis of possible connections between low-frequency wave propagation modes and the distribution of hydraulic properties. For sandstone intervals in the test well, the highest hydraulic conductivity intervals were typically correlated with the lowest phase velocities. The main characteristics of dispersion images obtained from the sand-screened well were highly comparable with those obtained at the same depth level in a nearby simple drillhole open to the formation. The sand-screened well and the open-hole displayed an expected and substantial difference between dispersion in sand- and clay-dominated intervals. It appears that for clay-dominated formations, the rate of change of phase velocity can be associated to clay content. We demonstrated that with appropriate acquisition and processing, multifrequency full-waveform sonic logging applied in existing large-diameter sand-screened wells can produce valuable results. There are few wireline logging technologies that can be applied in this setting. The techniques that we used would be highly suitable for time-lapse applications in high-volume production wells or for reassessing formation properties behind existing historical production wells.
Solvent-dependent Photoisomerization Quantum Yield of 2-Phenylazo-1-alkyl-3-methylimidazolium Cations in Ionic Liquids under S1(n, π*) Excitation
Authors: Yoshida Tsuyoshi , Monji Tomoo , Kawamori Daiki , Akai Nobuyuki , Shibuya Kazuhiko , Kawai Akio 
Published: September 2013

Abstract
Photoisomerization quantum yields (ΦE–Z) of phenylazo-substituted imidazolium cations, which are counterparts of recently developed photochromic ionic liquids, were measured under S1(n, π*) excitation. The ΦE–Z values are lower in imidazolium-type ionic liquids than those in conventional solvents and in tert-alkylammonium-type ionic liquids. This observation was argued based on solute–solvent complex formation at imidazolium rings.
Stress coping mechanisms in patients with chronic dermatoses.
Authors: Korabel Hanna , Grabski Bartosz , Dudek Dominika , Jaworek Andrzej , Gierowski Józef , Kiejna Andrzej , Wojas-Pelc Anna 
Published: September 2013

Aim of the studyThe results of numerous studies of today confirm that persons suffering from psychosomatic disorders are not able to effectively cope with stress. The experience of stress is also frequently combined with the occurrence or aggravation of various skin diseases.
The goal of our study was to identify the predominant ways of coping with stress in the group of patients with chronic dermatoses.Subject or material and methodsThe group under study included patients receiving treatment in the Dermatology Clinic of Collegium Medicum, Jagiellonian University. They were either hospitalized patients or those who came for control examinations at the Outpatient Clinic. Evaluation of the forms of coping with stress was conducted with the help of the Endler and Parker Questionnaire – CISS.Resultsstress. They significantly more often apply the style of coping focused on avoiding (p-value= 0.0056). It also turned out that the patients in the dermatological groups manifested a constant tendency to get involved in vicarious activities (p-value=0.0247).DiscussionThe results of the presented study indicate that there is a statistically significant difference between the patients with dermatological disorders and those in the control group as regards their ways of coping with stress.ConclusionsThe results obtained in the discussed study may be a starting point for designing a complex support for the patients with skin diseases. The therapeutic technique that may prove helpful for this group of patients is the cognitive-behavioral therapy (CTB).
Television: Horror’s ‘original’ home
Authors: Schmidt Lisa 
Published: September 2013

Abstract
Unquestionably, horror has always existed on television. Nevertheless, a number of scholars and critics have argued that the horror genre belongs properly to film, that television is too much a product of the industrial conditions that both regulate and create it to achieve ‘true’ horror. Not only is this position no longer viable (if it ever was) but it is based upon an assumption about what the horror genre is or should be. If we consider modern horror in relation to the Gothic novel and its immediate counterparts the stage melodrama and the sensation novel/drama, it soon becomes clear that television has a special and perhaps unique affinity with the genre as cultural and literary product. Television can do what film cannot, through its historical and contemporary strengths in the deployment of melodrama, particularly in serialized form. Through seriality, television horror reveals the melodramatic nature of the genre. Moreover, it (re)creates that original horror in a way that speaks, perhaps, to some of horror’s less appreciated pleasures and less acknowledged audiences. This article examines this claim through a sketch of Gothic and melodramatic traditions, tracing these through sensation novels, early film melodrama and ultimately contemporary serialized genre television, particularly the CW series Supernatural and The Vampire Diaries. In both series, the Gothic elements are manifested in the show’s manipulation of any and all elements of fantastic lore alongside plentiful gore and intense family drama.
The truth is in their faces: MTV’s Fear and the rise of ‘Personal Affect’ in paranormal horror
Authors: Zolides Andrew 
Published: September 2013

Abstract
Looking to capture the popularity and excitement surrounding the first truly successful ‘found footage’ horror film The Blair Witch Project (1999), in 2000 MTV began airing Fear, a reality competition-style television series that found contestants completing dares while exploring a haunted location to win money. Continuing the early tradition of MTV pushing boundaries and inventing formulas, Fear utilized the aesthetics made popular in Blair Witch and combined them with the increasingly popular reality competition format. While this union of seemingly disparate concepts did not last long in its own right, Fear set the standard for future paranormal reality television shows in terms of affective programming. Fear had a unique relationship between producer, consumer and technology, as the contestants filmed the show themselves, as well as utilizing surveillance cameras. This results in a more personal, amateur presentation that is indicative of a larger cultural trend in the twenty-first century, with a convergence of audience and performer, consumer and producer. Most interesting for Fear is that these lines are blurred not by ideology, but affect.