bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein predictive signatures for lymph node metastasis of gastric cancer
Authors: Li Wei , Ye Fei , Wang Daguang , Sun Xuan , Tong Weihua , Lian Guodong , Jiang Jing , Suo Jian , Zhang David Y. 
Published: September 2012

AbstractLymph node status remains one of most crucial indicators of gastric cancer prognosis and treatment planning. Current imaging methods have limited accuracy in predicting lymph node metastasis. We sought to identify protein markers in primary gastric cancer and to define a risk model to predict lymph node metastasis. The Protein Pathway Array (PPA) (initial selection) and Western blot (confirmation) were used to assess the protein expression in a total of 190 freshly frozen gastric cancer samples. The protein expression levels were compared between samples with lymph node metastasis (n = 73) and those without lymph node metastasis (n = 57) using PPA. There were 27 proteins differentially expressed between lymph node positive samples and lymph node negative samples. Five proteins (Factor XIII B, TFIIH p89, ADAM8, COX‐2 and CUL‐1) were identified as independent predictors of lymph node metastasis. Together with vascular/lymphatic invasion status, a risk score model was established to determine the risk of lymph node metastasis for each individual gastric cancer patient. The ability of this model to predict lymph node metastasis was further confirmed in a second cohort of gastric cancer patients (33 with and 27 without lymph node metastasis) using Western blot. This study indicated that some proteins differentially expressed in gastric cancer can be selected as clinically useful biomarkers. The risk score model is useful for determining patients' risk of lymph node metastasis and prognosis.
Early Postpartum Parental Preoccupation and Positive Parenting Thoughts: Relationship with Parent–Infant Interaction
Authors: Kim Pilyoung , Mayes Linda , Feldman Ruth , Leckman James F. , Swain James E. 
Published: September 2012

AbstractParenting behaviors and parent–infant emotional bonding during the early postpartum months play a critical role in infant development. However, the nature and progression of parental thoughts and their relationship with interactive behaviors have received less research. The current study investigated the trajectory of parental thoughts and behaviors among primiparous mothers (n = 18) and fathers (n = 15) and multiparous mothers (n = 13) and fathers (n = 13), which were measured at the first and third postpartum month. At the third postpartum month, the relationship between parental thoughts and parental interactive behaviors also was tested. Mothers and fathers showed high levels of preoccupations and caregiving thoughts during the first postpartum month that significantly declined by the third postpartum month. In contrast, positive thoughts about parenting and the infant increased over the same time interval. Mothers presented higher levels of preoccupations and positive thoughts than did fathers, and first‐time parents reported more intense preoccupations than did experienced parents. Although maternal sensitivity was inversely related to maternal anxious thoughts, paternal sensitivity was predicted by higher levels of anxious as well as caregiving and positive thoughts.
Surface modification of Thai silk fibroin scaffolds with gelatin and chitooligosaccharide for enhanced osteogenic differentiation of bone marrow‐derived mesenchymal stem cells
Authors: Wongputtaraksa Tanom , Ratanavaraporn Juthamas , Pichyangkura Rath , Damrongsakkul Siriporn 
Published: September 2012

AbstractIn this study, the surface modification of silk fibroin (SF) scaffolds with gelatin/chitooligosaccharide (G/COS) blends using the reaction of glutaraldehyde (GA) was established. The effects of G/COS mixing ratio (100/0, 90/10, 80/20, and 70/30) and GA crosslinking concentration (0.05, 0.10, 0.15, and 0.20 vol %) on the properties of scaffolds were investigated. At 0.10–0.20 vol % GA, all G/COS blends could be successfully conjugated on the SF scaffolds, as confirmed by the percentage of weight increased and the presence of functional groups indicating SF, G, and COS from FTIR spectra. Pore size of SF scaffolds was around 570 μm with 92% porosity, however, the G/COS‐conjugated SF scaffolds showed thickened pore's wall, smaller pore size (∼184–275 μm) and less porosity (∼81%), but increased density. This modified structure subsequently improved the compressive modulus of the G/COS‐conjugated SF scaffolds. In terms of biological properties, the gelatin‐conjugated SF scaffolds promoted the attachment and proliferation of bone marrow‐derived mesenchymal stem cells (MSC) rather than the other scaffolds. However, the G/COS‐conjugated SF scaffolds, particularly at the ratio of 70/30, promoted the osteogenic differentiation of MSC comparing to the SF scaffold, as confirmed by the production of alkaline phosphatase (ALP) activity and calcium (Ca), and the deposition of calcium phosphate (CaP). It was concluded that the G/COS‐conjugated SF scaffolds showed great mechanical properties due to the β‐structure of silk fibroin, as well as the enhanced biological properties due to the G/COS blends. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Association between survival in patients with primary invasive breast cancer and computer aided MRI
Authors: Dietzel Matthias , Zoubi Ramy , Vag Tibor , Gajda Mieczyslaw , Runnebaum Ingo B. , Kaiser Werner A. , Baltzer Pascal A. 
Published: September 2012

AbstractPurpose:To identify the potential of semi‐quantitative enhancement‐analysis in breast MRI to predict disease‐related death in primary breast cancer patients.Materials and Methods:The present study was planned and conducted according to international recommendations. All patients referred for pretherapeutic staging of primary breast cancer during 24 consecutive months were included into the study collective. They were followed‐up by our multidisciplinary breast center. For semi‐quantitative MRI‐analysis dedicated CAD‐software (computer assisted diagnosis) was used. Association between enhancement parameters and disease‐related survival was investigated using Cox proportional‐hazards ‐regression (CR).Results:A total of 115 patients were eligible for CR analysis. Median follow‐up time was 52 months. In 15 patients, disease‐related death occurred. CR analysis identified four enhancement parameters as independent and significant (P < 0.001) predictors of the endpoint. Coefficients were “Initial enhancement” (B = 0.0166), “Time to peak‐enhancement” (B = 1.0573), “Tumor volume” (B = 0.0175), and proportion of “tumor volume” showing “slow initial enhancement” followed by a “persistent” curve‐type (B = −0.0586).Conclusion:This study demonstrates the significant relationship between semi‐quantitative enhancement analysis in breast MRI and disease‐related death of breast cancer patients. As results were extracted from a routine staging examination, MRI noninvasively provides not only diagnostic information but also outcome data at one step. Future studies should address the impact of these findings on patient management and therapeutic approach. J. Magn. Reson. Imaging 2013;37:146–155. © 2012 Wiley Periodicals, Inc.
Automatic intra‐subject registration‐based segmentation of abdominal fat from water–fat MRI
Authors: Joshi Anand A. , Hu Houchun H. , Leahy Richard M. , Goran Michael I. , Nayak Krishna S. 
Published: September 2012

AbstractPurpose:To develop an automatic registration‐based segmentation algorithm for measuring abdominal adipose tissue depot volumes and organ fat fraction content from three‐dimensional (3D) water–fat MRI data, and to evaluate its performance against manual segmentation.Materials and Methods:Data were obtained from 11 subjects at two time points with intermediate repositioning, and from four subjects before and after a meal with repositioning. Imaging was performed on a 3 Tesla MRI, using the IDEAL chemical‐shift water–fat pulse sequence. Adipose tissue (subcutaneous—SAT, visceral—VAT) and organs (liver, pancreas) were manually segmented twice for each scan by a single trained observer. Automated segmentations of each subject's second scan were generated using a nonrigid volume registration algorithm for water–fat MRI images that used a b‐spline basis for deformation and minimized image dissimilarity after the deformation. Manual and automated segmentations were compared using Dice coefficients and linear regression of SAT and VAT volumes, organ volumes, and hepatic and pancreatic fat fractions (HFF, PFF).Results:Manual segmentations from the 11 repositioned subjects exhibited strong repeatability and set performance benchmarks. The average Dice coefficients were 0.9747 (SAT), 0.9424 (VAT), 0.9404 (liver), and 0.8205 (pancreas); the linear correlation coefficients were 0.9994 (SAT volume), 0.9974 (VAT volume), 0.9885 (liver volume), 0.9782 (pancreas volume), 0.9996 (HFF), and 0.9660 (PFF). When comparing manual and automated segmentations, the average Dice coefficients were 0.9043 (SAT volume), 0.8235 (VAT), 0.8942 (liver), and 0.7168 (pancreas); the linear correlation coefficients were 0.9493 (SAT volume), 0.9982 (VAT volume), 0.9326 (liver volume), 0.8876 (pancreas volume), 0.9972 (HFF), and 0.8617 (PFF). In the four pre‐ and post‐prandial subjects, the Dice coefficients were 0.9024 (SAT), 0.7781 (VAT), 0.8799 (liver), and 0.5179 (pancreas); the linear correlation coefficients were 0.9889, 0.9902 (SAT, and VAT volume), 0.9523 (liver volume), 0.8760 (pancreas volume), 0.9991 (HFF), and 0.6338 (PFF).Conclusion:Automated intra‐subject registration‐based segmentation is potentially suitable for the quantification of abdominal and organ fat and achieves comparable quantitative endpoints with respect to manual segmentation. J. Magn. Reson. Imaging 2013;37:423–430. © 2012 Wiley Periodicals, Inc.
Auto‐Threshold quantification of late gadolinium enhancement in patients with acute heart disease
Authors: Vermes Emmanuelle , Childs Helene , Carbone Iacopo , Barckow Philipp , Friedrich Matthias G. 
Published: September 2012

AbstractPurpose:To assess the Otsu‐Auto‐Threshold (OAT) for accuracy and reproducibility for sizing irreversible injury in late gadolinium enhancement (LGE) images of patients with acute heart disease. The OAT method automatically identifies high signal intensity areas using a cutoff derived from the signal intensity histogram and therefore is user‐independent.Materials and Methods:LGE was performed in 28 patients with acute myocardial infarction (MI) and 30 patients with acute myocarditis. LGE mass was compared between OAT and thresholds using 2 standard deviations (SD), 3SD, and 5SD above remote myocardium, and full‐width‐at‐half‐maximum (FWHM). A separate, blinded visual assessment served as the standard of truth.Results:In patients with acute MI, OAT and 5SD did not differ (26.1 ± 11.4 g vs. 25.4 ± 11.1 g, P = 0.088), but thresholds of 2SD and 3SD overestimated LGE mass by 37% and 20%, respectively, and FWHM underestimated by 15%. In acute myocarditis, OAT was not different from a visual quantification, but thresholds of 2SD and 3SD overestimated LGE mass by 46% and 19%, respectively, and thresholds of 5SD and FWHM underestimated LGE mass by 17% and 26%, respectively. OAT and FWHM showed the best intraobserver and interobserver reproducibility.Conclusion:Automatic thresholding using OAT may serve as an accurate and reproducible method to quantify irreversible myocardial injury in acute heart disease. J. Magn. Reson. Imaging 2013;37:382–390. © 2012 Wiley Periodicals, Inc.
Functional MRI of the thoracic spinal cord during vibration sensation
Authors: Kornelsen Jennifer , Smith Stephen D. , McIver Theresa A. , Sboto‐Frankenstein Uta , Latta Peter , Tomanek Boguslaw 
Published: September 2012

AbstractPurpose:To demonstrate that it is possible to acquire accurate functional magnetic resonance images from thoracic spinal cord neurons.Materials and Methods:The lower thoracic spinal dermatomes (T7–T11) on the right side of the body were mechanically stimulated by vibration for 15 participants. Neuronal responses to vibration sensation were measured in the thoracic spinal cord using a HASTE sequence on a 3 Tesla MRI system.Results:Signal increases were observed in the corresponding lower thoracic spinal cord segments ipsilateral to the side of stimulation in the dorsal aspect of the spinal cord.Conclusion:This is the first study to provide proof of principle that functional imaging of the entire thoracic spinal cord is possible, by detecting neuronal activity in the thoracic spinal cord during sensory stimulation using spinal fMRI. J. Magn. Reson. Imaging 2013;37:981–985. © 2012 Wiley Periodicals, Inc.
Contrast‐enhanced specific absorption rate‐efficient 3D cardiac cine with respiratory‐triggered radiofrequency gating
Authors: Henningsson Markus , Chan Raymond H. , Goddu Beth , Goepfert Lois A. , Razavi Reza , Botnar Rene M. , Schaeffter Tobias , Nezafat Reza 
Published: September 2012

AbstractPurpose:To investigate the use of radiofrequency (RF) gating in conjunction with a paramagnetic contrast agent to reduce the specific absorption rate (SAR) and increase the blood‐myocardium contrast in balanced steady‐state free precession (bSSFP) 3D cardiac cine.Materials and Methods:RF gating was implemented by synchronizing the RF‐excitation with an external respiratory sensor (bellows), which could additionally be used for respiratory gating. For reference, respiratory‐gated 3D cine images were acquired without RF gating. Free‐breathing 3D cine images were acquired in eight healthy subjects before and after contrast injection (Gd‐BOPTA) and compared to breath‐hold 2D cine.Results:RF‐gated 3D cine reduced the SAR by nearly 40% without introducing significant artifacts while providing left ventricle (LV) measurements similar to those obtained with 2D cine. The contrast‐to‐noise ratio (CNR) was significantly higher for 3D cine compared to 2D cine, both before and after contrast injection; however, no statistically significant CNR increase was observed for the postcontrast 3D cine compared to the precontrast acquisitions.Conclusion:Respiratory‐triggered RF gating significantly reduces SAR in 3D cine acquisitions, which may enable a more widespread clinical use of 3D cine. Furthermore, CNR of 3D bSSFP cine is higher than of 2D and administration of Gd‐BOPTA does not improve the CNR of 3D cine. J. Magn. Reson. Imaging 2013;37:986–992. © 2012 Wiley Periodicals, Inc.
Synthesis of prebiotic carbohydrates derived from cheese whey permeate by a combined process of isomerisation and transgalactosylation
Authors: Corzo‐Martínez Marta , Copoví Paula , Olano Agustín , Moreno F Javier , Montilla Antonia 
Published: September 2012

AbstractBackgroundLactose from cheese whey permeate (WP) was efficiently isomerised to lactulose using egg shell, a food‐grade catalyst, and the subsequent transgalactosylation reaction of this mixture with β‐galactosidase from Bacillus circulans gave rise to a wide array of prebiotic carbohydrates derived from lactose and lactulose.ResultsLactulose obtained by efficient isomerisation of WP (16.1% by weight with respect to the initial amount of lactose) showed great resistance to the hydrolytic action of β‐galactosidase from B. circulans, which preferentially hydrolysed lactose, acting as a galactosyl donor and acceptor. Lactulose had capacity as an acceptor, leading to the formation of lactulose‐derived oligosaccharides. The enzymatic synthesis was optimised by studying reaction conditions such as pH, temperature, time, enzyme concentration and carbohydrate concentration. The maximum formation of galactooligosaccharides with degrees of polymerisation from 2 to 4 was achieved after 5 h of reaction at pH 6.5 and 50 °C with 300 g kg−1 carbohydrates and 3 U mL−1 β‐galactosidase.ConclusionThese findings indicate that the transgalactosylation of isomerised WP with β‐galactosidase from B. circulans could be a new and efficient method to obtain a mixture with 50% of potentially prebiotic carbohydrates composed of lactulose, and galactooligosaccharides derived from lactose and lactulose.
Magnetically modified spent grain as a low‐cost, biocompatible and smart carrier for enzyme immobilisation
Authors: Pospiskova Kristyna , Safarik Ivo 
Published: September 2012

AbstractBackgroundFood and feed technology and biotechnology benefit from the use of immobilised enzymes. New low‐cost enzyme carriers exhibiting high biocompatibility and response to external magnetic field can substantially improve the application potential of immobilised enzyme systems.ResultsFerrofluid‐modified spent grain was used as a low‐cost, biocompatible and magnetically responsive carrier for the immobilisation of Candida rugosa lipase. Several immobilisation procedures were tested using both native and poly(ethyleneimine)‐modified magnetic spent grain. Activity of immobilised lipase per unit mass of carrier, operational stability, time stability and Michaelis constant were compared. In general, magnetic spent grain modified with poly(ethyleneimine) bound a smaller amount of active lipase than unmodified magnetic spent grain, but the operational and storage stabilities of enzyme immobilised on poly(ethyleneimine)‐modified carrier were very high.ConclusionFerrofluid‐modified spent grain can be a promising low‐cost magnetic carrier for enzyme immobilisation, applicable e.g. in food and feed technology and biotechnology.© 2012 Society of Chemical Industry
Effects of vacuum packing on eggshell microbial activity and egg quality in table eggs under different storage temperatures
Authors: Aygun Ali , Sert Durmus 
Published: September 2012

AbstractBackroundThe aim of this study was to establish the effects of vacuum packing on eggshell microbial activity and egg quality traits in table eggs during 42 days of storage at 5 and 22 °C. Treatments were no vacuum packing (control) and vacuum packing (VP). Egg quality traits measured included egg weight loss, specific gravity, shell strength, albumen height, Haugh unit, yolk index, albumen pH, yolk pH, albumen colour and yolk colour.ResultsVP eggs maintained higher specific gravity, albumen height, Haugh unit and yolk index and lower egg weight loss, albumen pH and yolk pH compared with control eggs after 42 days at 22 °C. VP eggs had lower levels of total aerobic mesophilic bacteria, Salmonella spp., Staphylococcus spp. and moulds/yeasts than control eggs over the storage period at both 5 and 22 °C. However, VP eggs had a higher level of coliforms than control eggs after 42 days at 5 °C.ConclusionThe results indicated that vacuum packing extended the egg shelf life to at least 42 days compared with control eggs at 5 and 22 °C.
Synthesis of Novel Bis(β‐enaminoketonato)titanium Catalyst with High Activity and Excellent Ability to Copolymerize Olefins
Authors: Yang Guofan , Hong Miao , Li Yuesheng , Yu Shijun 
Published: September 2012

AbstractA new titanium complex [PhN=C(Ph)CHC(CF3)O]2TiCl2 (2a), with an asymmetric β‐enaminoketonato [N,O] ligand, has been synthesized and fully characterized. With modified methylaluminoxane as a cocatalyst, the behaviors of ethylene polymerization, and ethylene/α‐olefin and ethylene/norbornene copolymerization have been explored in detail. Relative to its regioisomer 2b ([PhN=C(CF3)CHC(Ph)O]2TiCl2), the present catalyst exhibits significant predominance. The catalytic activity in 2a‐catalyzed homo‐ and copolymerization are much higher than those in 2b‐catalyzed (co)polymerization. Furthermore, compared with 2b, catalyst 2a possesses an obviously stronger ability towards copolymerization, due to a wide coordination space caused by exchanging the position of the phenyl group and trifluoromethyl group on the framework.
Providing care to meet complex needs: A cross‐sectorial approach
Authors: Cripps Sue , Spencer Margaret , Clark Andrew 
Published: September 2012

AbstractThis is a case study of a young man with a mild intellectual disability and schizophrenia, who as a result of engaging in high‐risk behaviour acquired HIV and subsequently developed AIDS dementia. This resulted in a crisis of care that could not be resolved by traditional diagnostic based clinical services. A care package was developed by the authors that focused on the young man's needs, while embracing the principles of harm minimisation and the least restrictive environment. The authors argue that it is only through the development of a cross‐sectorial approach based on the needs of the individual that the needs of people with complex care issues can be met.
Parental informed consent in pediatric cancer trials: A population‐based survey in Germany
Authors: Petersen Imme , Spix Claudia , Kaatsch Peter , Graf Norbert , Janka Gritta , Kollek Regine 
Published: September 2012

AbstractBackgroundEnsuring adequate parental consent is a key issue of ethical practice in pediatric oncology. In Germany, however, knowledge about parental comprehension and satisfaction with the informed consent procedure is limited, and representative data on parents' perspectives are still missing. Based on data collected by means of a population‐based survey, we evaluated the parental recall of the informed consent process for pediatric clinical trials, and how they rated the consent process retrospectively.ProcedureA standardized survey was carried out among 1,465 parents whose children were first diagnosed in 2005 with a disease defined by ICCC‐3 in the German Childhood Cancer Registry (response: 55.1%). The survey's primary objective was to assess how well parents were able to recall of the informed consent process. To evaluate the results, we set up a second survey among 581 parents who had given consent recently for their child's participation in a clinical trial (response: 53.5%).ResultsApproximately 81% of the parents in the population‐based survey correctly remembered whether or not their child had been enrolled in a clinical trial or treated off‐trial. The ability to recall accurately is significantly lower if the parents have a migration background or if their child was not a trial participant. However, parents who recalled the child's trial participation status incorrectly felt as adequately informed as parents who recalled it correctly.ConclusionsOur results identified weak points and vulnerable subgroups in the parental consent process in pediatric oncology in Germany. Pediatr Blood Cancer 2013; 60: 446–450. © 2012 Wiley Periodicals, Inc.
Dicarboxylic acid promoted immortal copolymerization for controllable synthesis of low‐molecular weight oligo(carbonate‐ether) diols with tunable carbonate unit content
Authors: Gao Yonggang , Gu Lin , Qin Yusheng , Wang Xianhong , Wang Fosong 
Published: September 2012

AbstractLow‐molecular weight oligo(carbonate‐ether) diols are important raw materials for polyurethane formation, which with tunable carbonate unit content (CU) may endow new thermal and mechanical performances to polyurethane. Herein, facile synthesis of oligo(carbonate‐ether) diols with number average molecular weight (Mn) below 2000 g mol−1 and CU tunable between 40% and 75% are realized in high activity by immortal copolymerization of CO2/propylene oxide (PO) using zinc‐cobalt double metal cyanide complex (Zn‐Co‐DMCC) in the presence of sebacic acid (SA). Mn of the oligomer is in good linear relationship to the mole ratio of PO and SA (PO/SA) and hence can be precisely controlled by adjusting PO/SA. Besides, the molecular weight distribution is quite narrow due to the rapid reversible chain transfer in the immortal copolymerization. High pressure and low temperature are favorable for raising CU. In all the reactions, the weight fraction of propylene carbonate (WPC) can even be controlled as low as 2.0 wt %, and the catalytic activity of Zn‐Co‐DMCC is above 1.0 kgg−1 cat. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012