bluuebunny/crossref_metadata_2025_split huggingface dataset:
Point-of-care ultrasound training in nephrology: a position statement by the International Alliance for POCUS in Nephrology
Authors: Koratala Abhilash , Argaiz Eduardo R , Romero-González Gregorio , Reisinger Nathaniel , Anwar Siddiq , Beaubien-Souligny William , Bhasin-Chhabra Bhavna , Diniz Hugo , Vaca Gallardo Marco , Graterol Torres Fredzzia , Husain-Syed Faeq , Hanko Jennifer , Jaberi Aala , Kazory Amir , Raina Rupesh , Ronco Claudio , Salgado Octavio J , Sethi Sidharth Kumar , Villavicencio-Cerón Vanessa , Yadla Manjusha , Bastos Marcus Gomes 
Published: August 2024

ABSTRACT
Point-of-care ultrasonography (POCUS) has rapidly evolved from a niche technology to an indispensable tool across medical specialties, including nephrology. This evolution is driven by advancements in technology and the visionary efforts of clinicians in emergency medicine and beyond. Recognizing its potential, medical schools are increasingly integrating POCUS into training curricula, emphasizing its role in enhancing diagnostic accuracy and patient care. Despite these advancements, barriers such as limited faculty expertise and ‘lack of’ standardized guidelines hinder widespread adoption and regulation. The International Alliance for POCUS in Nephrology (IAPN), through this position statement, aims to guide nephrologists in harnessing the diagnostic power of POCUS responsibly and effectively. By outlining core competencies, recommending training modalities and advocating for robust quality assurance measures, we envision a future where POCUS enhances nephrology practice globally, ensuring optimal patient outcomes through informed, evidence-based decision-making. International collaboration and education are essential to overcome current challenges and realize the full potential of POCUS in nephrology and beyond.
Spiny dogfish, Squalus suckleyi, shows a good tolerance for hypoxia but need long recovery times
Authors: De Boeck Gudrun , Lardon Isabelle , Eyckmans Marleen , Vu Trung Nghia , Laukens Kris , Dommisse Roger , Wood Chris M 
Published: August 2024

Abstract
Pacific spiny dogfish, Squalus suckleyi, move to shallow coastal waters during critical reproductive life stages and are thus at risk of encountering hypoxic events which occur more frequently in these areas. For effective conservation management, we need to fully understand the consequences of hypoxia on marine key species such as elasmobranchs. Because of their benthic life style, we hypothesized that S. suckleyi are hypoxia tolerant and able to efficiently regulate oxygen consumption, and that anaerobic metabolism is supported by a broad range of metabolites including ketones, fatty acids and amino acids. Therefore, we studied oxygen consumption rates, ventilation frequency and amplitude, blood gasses, acid–base regulation, and changes in plasma and tissue metabolites during progressive hypoxia. Our results show that critical oxygen levels (Pcrit) where oxyregulation is lost were indeed low (18.1% air saturation or 28.5 Torr at 13°C). However, many dogfish behaved as oxyconformers rather than oxyregulators. Arterial blood PO2 levels mostly decreased linearly with decreasing environmental PO2. Blood gases and acid–base status were dependent on open versus closed respirometry but in both set-ups ventilation frequency increased. Hypoxia below Pcrit resulted in an up-regulation of anaerobic glycolysis, as evidenced by increased lactate levels in all tissues except brain. Elasmobranchs typically rely on ketone bodies as oxidative substrates, and decreased concentrations of acetoacetate and β-hydroxybutyrate were observed in white muscle of hypoxic and/or recovering fish. Furthermore, reductions in isoleucine, glutamate, glutamine and other amino acids were observed. After 6 hours of normoxic recovery, changes persisted and only lactate returned to normal in most tissues. This emphasizes the importance of using suitable bioindicators adjusted to preferred metabolic pathways of the target species in conservation physiology. We conclude that Pacific spiny dogfish can tolerate severe transient hypoxic events, but recovery is slow and negative impacts can be expected when hypoxia persists.
Presentation and outcomes of women and men undergoing surgery for degenerative mitral regurgitation
Authors: van Kampen Antonia , Butte Sophie , Paneitz Dane C , Nagata Yasufumi , Langer Nathaniel B , Borger Michael A , D’Alessandro David A , Sundt Thoralf M , Melnitchouk Serguei 
Published: August 2024

Abstract

OBJECTIVES
Degenerative mitral regurgitation is associated with heart failure, arrhythmia and mortality. The impact of sex on timing of surgical referral and outcomes has not been reported comprehensively. We examined preoperative status and surgical outcomes of male versus female degenerative mitral valve regurgitation patients undergoing surgery.

METHODS
We reviewed our institutional database for all patients undergoing surgery for degenerative mitral regurgitation between 2013 and 2021. Preoperative clinical and echocardiographic variables, surgical characteristics and outcomes were compared, and left atrial strain in available images.

RESULTS
Of 963 patients, 314 (32.6%) were female. Women were older (67 vs 64 years, P = 0.031) and more often had bileaflet prolapse (19.4% vs 13.8%, P = 0.028), mitral annular calcification (12.1% vs 5.4%, P &lt; 0.001) and tricuspid regurgitation (TR; 31.8% vs 22.5%, P = 0.001). Indexed left ventricular end-diastolic and end-systolic diameters were higher in women, with 29.4 vs 26.7 mm/m2 (P &lt; 0.001) and 18.2 vs 17 mm/m2 (P &lt; 0.001), respectively, and left atrial conduit strain lower (17.6% vs, 21.2%, P = 0.001). Predicted risk of mortality was 0.73% vs 0.54% in men (P = 0.023). Women required mechanical circulatory support more frequently (1.3% vs 0%, P = 0.011), had longer intensive care unit stay (29 vs 26 h, P &lt; 0.001), mechanical ventilation (5.4 vs 5 h, P = 0.036), and overall hospitalization (7 vs 6 days, P &lt; 0.001). There was no difference in long-term reoperation-free survival (P = 0.35).

CONCLUSIONS
Women undergoing mitral valve repair are older and show indicators of more advanced disease with long-standing left ventricular impairment. Guidelines may need to be adjusted and address this disparity, to improve postoperative recovery times and outcomes.
Antibacterial mode of action of garviecin LG34 against Gram-negative bacterium Salmonella typhimurium
Authors: Gao Yurong , Li Dapeng 
Published: August 2024

Abstract
Garviecin LG34 produced by Lactococcus garvieae LG34 exhibits wide-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria. This work aimed at clarifying the antibacterial mode of action of garviecin LG34 against Gram-negative bacterium Salmonella typhimurium. To determine the concentration for the bacteriocin antimicrobial mode experiments, the minimum inhibitory concentration of garviecin LG34 against S. typhimurium CICC21484 was determined as 0.25 mg/ml. Garviecin LG34 decreased the viable count of S. typhimurium CICC21484 and its antibacterial activity was the dose and time dependant. Garviecin LG34 led to the dissipation of transmembrane potential, the rise in the extracellular conductivity, UV-absorbing material at 260 nm, and LDH level of S. typhimurium CICC21484. Scanning electron micrographs results shown that garviecin LG34 cause dramatic deformation and fragmentation including the flagellum shedding, pores formation in surface, and even completely breakage of S. typhimurium cell. Moreover, garviecin LG34 decreased the intracellular ATP level. The results of this study demonstrated that garviecin LG34 can destroy cell structure, increase membrane permeability of S. typhimurium, thereby might be used as biopreservative for treating food borne and salmonellosis resulting from Gram-negative bacterium S. typhimurium.
Redefining the Successful Aging of Veterans: A Scoping Review
Authors: Burns Kate Hutton , Neves Barbara Barbosa , Warren Narelle 
Published: August 2024

Abstract

Background and Objectives
The aging experiences of military veterans provide critical insights into what successful aging is and means for later life contexts constrained by distinct health and social needs. Can veterans “successfully” age when they are exposed to so many stressors with serious health and social consequences for later life? Veterans can offer valuable lessons for developing comprehensive approaches to refining successful aging, ensuring inclusivity of different older populations. Building on Rowe and Kahn’s idea of successful aging, we utilize the complementary concept of “active aging” to explore if there are unique factors, characteristics, and interventions that support active aging in veterans, compared with nonveteran populations.

Research Design and Methods
A rapid review and evidence synthesis was conducted across 9 databases in medicine, psychology, anthropology, sociology, and public health to search for peer-reviewed articles and research reports.

Results
Findings suggest that programs linking health and social dimensions can support the active aging of veterans, namely interventions promoting active physical and cognitive lifestyle as well as social connectedness and engagement. Such programs and interventions help prevent and combat mental and physical health decline and increase quality of life and well-being.

Discussion and Implications
Findings have implications for veteran and nonveteran populations more broadly, as people can actively age even when they have unique health and social needs.
Starting conversations about mental health and wellbeing in Australian culturally and linguistically diverse communities
Authors: Morse Alyssa R , Smith Dianna G , Clifford Rosemary , Shrimpton Brad , Banfield Michelle 
Published: August 2024

Abstract
Australia is a multicultural nation with nearly 30% of the population born overseas. Migrants’ mental health can be impacted by discrimination, racism and experiences relating to asylum and immigration. These can be compounded by low help-seeking caused by stigmatized beliefs and poor mental health literacy. My Mind, My Voice (MMMV) is a co-designed program aiming to promote awareness of mental health and wellbeing for people with a culturally and linguistically diverse (CALD) background. This research project explored the perceived impacts and value of MMMV and processes leading to those impacts. A mixture of internal quantitative and qualitative evaluation surveys (n = 32) and researcher-conducted semi-structured interviews (n = 9) were conducted with CALD organization and community members who attended training workshops, presented MMMV events or attended an event. Data were analysed using a reflexive thematic analysis approach. Five themes were developed: culturally relevant and respectful, cross-cultural connections, the importance of language, increasing confidence and literacy and the potential to change attitudes. Being involved with a co-produced program that was culturally relevant and respectful was a positive experience that enhanced people’s confidence and literacy. Feeling respected, valued and validated helped participants feel empowered to develop and deliver mental health and wellbeing education in their community. Open, honest conversations are an important way to break down stigma and start conversations about mental health and wellbeing in CALD communities. Evaluation outcomes demonstrate the success of MMMV’s collaborative approach, which can inform the development and evaluation of CALD mental health promotion interventions.
Δ9-Tetrahydrocannabinol and 11-hydroxy-Δ9-tetrahydrocannabinol as markers of cannabis use in urinary drug testing
Authors: Vikingsson Svante , Winecker Ruth E , Cone Edward J , Kuntz David J , Hayes Eugene D , Flegel Ronald R , Davis Lisa S 
Published: August 2024

Abstract
With some exceptions, California Assembly Bill 2188 will preclude the use of ∆9-tetrahydrocannabinol-9-carboxylic acid (Δ9-THC-COOH) as a marker of cannabis use in urinary workplace drug testing. The bill allows for the use of psychoactive cannabis markers, which include Δ9-tetrahydrocannabinol (Δ9-THC) and the metabolite 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-Δ9-THC). Both analytes are present in urine mainly as conjugated metabolites and will require hydrolysis prior to analysis, but very little is known about expected concentrations in urine. The aim of this study was to report the concentrations from two large datasets comprising 1411 workplace drug-testing urine specimens that tested positive by immunoassay (50 ng/mL cutoff) and discuss strategies for using 11-OH-Δ9-THC and/or Δ9-THC to detect cannabis use. Median 11-OH-Δ9-THC and Δ9-THC concentrations were 28%–35% and 1.1%–1.6% of those of Δ9-THC-COOH, respectively, and correlations between the analytes were observed. To avoid the risk of positives from passive exposure, laboratories could use a cutoff with equivalent sensitivity to cannabis exposure. A 5 ng/mL cutoff for 11-OH-Δ9-THC showed 92% agreement with a 15 ng/mL cutoff for Δ9-THC-COOH, with only 0.9% of specimens being positive only for 11-OH-Δ9-THC. It was not possible to propose an estimated cutoff for Δ9-THC, due to the constraints of the limit of detection used in this study.
Mating frequency estimation and its importance for colony abundance analyses in eusocial pollinators: a case study of Bombus impatiens (Hymenoptera: Apidae)
Authors: Bird Sydney A , Pope Nathaniel S , McGrady Carley M , Fleischer Shelby J , López-Uribe Margarita M 
Published: August 2024

Abstract
The genus Bombus (bumble bees) includes approximately 265 species, many of which are in decline in North America and Europe. To estimate colony abundance of bumble bees in natural and agricultural habitats, sibship relationships are often reconstructed from genetic data with the assumption that colonies have 1 monandrous queen. However, some species such as the North American common eastern bumble bee (Bombus impatiens Cresson) can display low levels of polyandry, which may bias estimates of colony abundance based on monandrous sibship reconstructions. To accurately quantify rates of polyandry in wild and commercially mated queens of this species, we empirically estimated mating frequencies using a novel statistical model and genotypes from 730 bees. To genotype individuals, we used a highly polymorphic set of microsatellites on colonies established from 20 wild-caught gynes and 10 commercial colonies. We found multiple fathers in 3 of the wild colonies and 3 of the commercial colonies. This resulted in average effective mating frequencies of 1.075 ± 0.18 and 1.154 ± 0.25 for wild and commercial colonies, respectively. These findings agree with previous reports of low rates of polyandry for B. impatiens. Using a large empirical dataset, we demonstrate that assuming monandry for colony abundance estimation in species that violate this assumption results in an overestimation of the number of colonies. Our results emphasize the importance of studying mating frequencies in social species of conservation concern and economic importance for the accuracy of colony abundance estimation and for understanding their ecology and sociobiology.
Multiband polarimetric imaging of HD 34700 with SCExAO/CHARIS
Authors: Chen Minghan , Lawson Kellen , Brandt Timothy D , Lewis Briley L , Uyama Taichi , Millar-Blanchaer Max , Tazaki Ryo , Currie Thayne 
Published: August 2024

ABSTRACT
We present Subaru/SCExAO + CHARIS broad-band (JHK) integral field spectroscopy of HD 34700 A in polarized light. CHARIS has the unique ability to obtain polarized integral field images at 22 wavelength channels in broad-band, as the incoming light is first split into different polarization states before passing though the lenslet array. We recover the transition disc around HD 34700 A in multiband polarized light in our data. We combine our polarized intensity data with previous total intensity data to examine the scattering profiles, scattering phase functions and polarized fraction of the disc at multiple wavelengths. We also carry out three-dimensional Monte Carlo radiative transfer simulations of the disc using mcfost, and make qualitative comparisons between our models and data to constrain dust grain properties. We find that in addition to micron-sized dust grains, a population of submicron grains is needed to match the surface brightness in polarized light and polarized fraction. This could indicate the existence of a population of small grains in the disc, or it could be caused by Mie theory simulations using additional small grains to compensate for submicron structures of real dust aggregates. We find models that match the polarized fraction of the data but the models do not apply strong constraints on the dust grain type or compositions. We find no models that can match all observed properties of the disc. More detailed modelling using realistic dust aggregates with irregular surfaces and complex structures is required to further constrain the dust properties.
Inequality Bands: Seventy-five years of measuring income inequality in Latin America
Authors: Alvaredo Facundo , Bourguignon François , Ferreira Francisco , Lustig Nora 
Published: August 2024

Abstract
Drawing on a comprehensive compilation of quantile shares and inequality measures for 34 countries, including over 5600 estimated Gini coefficients, we review the measurement of income inequality in Latin America and the Caribbean over the last seven decades. We find that there is quite a bit of uncertainty regarding inequality levels for the same country/year combinations. Differences in inequality levels estimated from household surveys alone are present but they derive from differences in the construction of the welfare indicator, the unit of analysis, or the treatment of the data. With harmonized household surveys, the discrepancies are quite small. The range, however, expands significantly when –to correct for undercoverage and underreporting especially at the top of the distribution– inequality estimates come from some combination of surveys and administrative tax data. The range increases even further when survey-based income aggregates are scaled to achieve consistency not only with tax registries but with National Accounts. Since no single method to correct for underreporting at the top is fully convincing at present, we are left with (often wide) ranges, or bands, of inequality as our best summaries of inequality levels. Reassuringly, however, the dynamic patterns are generally robust across the bands. Although the evidence roughly until the 1970s is too fragmentary and difficult to compare, clearer patterns emerge for the last fifty years. The main feature is a broad inverted U curve, with inequality rising in most countries prior to and often during the 1990s, and falling during the early 21st century, at least until around 2015, when trends appear to diverge across countries. This pattern is broadly robust but features considerable variation in timing and magnitude depending on the country.
Ribosome stalling during c-myc translation presents actionable cancer cell vulnerability
Authors: Khaket Tejinder Pal , Rimal Suman , Wang Xingjun , Bhurtel Sunil , Wu Yen-Chi , Lu Bingwei 
Published: August 2024

Abstract
Myc is a major driver of tumor initiation, progression, and maintenance. Up-regulation of Myc protein level rather than acquisition of neomorphic properties appears to underlie most Myc-driven cancers. Cellular mechanisms governing Myc expression remain incompletely defined. In this study, we show that ribosome-associated quality control (RQC) plays a critical role in maintaining Myc protein level. Ribosomes stall during the synthesis of the N-terminal portion of cMyc, generating aberrant cMyc species and necessitating deployment of the early RQC factor ZNF598 to handle translational stress and restore cMyc translation. ZNF598 expression is up-regulated in human glioblastoma (GBM), and its expression positively correlates with that of cMyc. ZNF598 knockdown inhibits human GBM neurosphere formation in cell culture and Myc-dependent tumor growth in vivo in Drosophila. Intriguingly, the SARS-COV-2-encoded translational regulator Nsp1 impinges on ZNF598 to restrain cMyc translation and consequently cMyc-dependent cancer growth. Remarkably, Nsp1 exhibits synthetic toxicity with the translation and RQC-related factor ATP-binding cassette subfamily E member 1, which, despite its normally positive correlation with cMyc in cancer cells, is co-opted by Nsp1 to down-regulate cMyc and inhibit tumor growth. Ribosome stalling during c-myc translation thus offers actionable cancer cell vulnerability.
The Third Family Quark Mass Hierarchy and FCNC in the Universal Seesaw Model
Authors: Morozumi Takuya , Panuluh Albertus Hariwangsa 
Published: August 2024

Abstract
We present the study of the quark sector of the universal seesaw model with $\mathrm{SU(2)_L \times SU(2)_R \times U(1)_{Y^{\prime }}}$ gauge symmetry in the massless case of the two lightest quark families. This model aims to explain the mass hierarchy of the third family quark by introducing a vector-like quark (VLQ) partner for each quark. In this model, we introduce $\mathrm{SU(2)_L}$ and $\mathrm{SU(2)_R}$ Higgs doublets. We derive explicitly the Lagrangian for the quark sector, Higgs sector, and kinetic terms of the gauge fields, starting from the Lagrangian, which is invariant under $\mathrm{SU(2)_L \times SU(2)_R \times U(1)_{Y^{\prime }}}$ gauge symmetry. At each stage of the symmetry breaking, we present the Lagrangian with the remaining gauge symmetry. Additionally, we investigate the flavor-changing neutral currents (FCNCs) of the Higgs (h) and Z-bosons in the interaction with the top, heavy top, bottom, and heavy bottom quarks.
The role of collagen and crystallinity in the physicochemical properties of naturally derived bone grafts
Authors: Øvrebø Øystein , Orlando Luca , Rubenis Kristaps , Ciriello Luca , Ma Qianli , Giorgi Zoe , Tognoni Stefano , Loca Dagnija , Villa Tomaso , Nogueira Liebert P , Rossi Filippo , Haugen Håvard J , Perale Giuseppe 
Published: August 2024

Abstract
Xenografts are commonly used for bone regeneration in dental and orthopaedic domains to repair bone voids and other defects. The first-generation xenografts were made through sintering, which deproteinizes them and alters their crystallinity, while later xenografts are produced using cold-temperature chemical treatments to maintain the structural collagen phase. However, the impact of collagen and the crystalline phase on physicochemical properties have not been elucidated. We hypothesized that understanding these factors could explain why the latter provides improved bone regeneration clinically. In this study, we compared two types of xenografts, one prepared through a low-temperature chemical process (Treated) and another subsequently sintered at 1100°C (Sintered) using advanced microscopy, spectroscopy, X-ray analysis and compressive testing. Our investigation showed that the Treated bone graft was free of residual blood, lipids or cell debris, mitigating the risk of pathogen transmission. Meanwhile, the sintering process removed collagen and the carbonate phase of the Sintered graft, leaving only calcium phosphate and increased mineral crystallinity. Microcomputed tomography revealed that the Treated graft exhibited an increased high porosity (81%) and pore size compared to untreated bone, whereas the Sintered graft exhibited shrinkage, which reduced the porosity (72%), pore size and strut size. Additionally, scanning electron microscopy displayed crack formation around the pores of the Sintered graft. The Treated graft displayed median mechanical properties comparable to native cancellous bone and clinically available solutions, with an apparent modulus of 166 MPa, yield stress of 5.5 MPa and yield strain of 4.9%. In contrast, the Sintered graft exhibited a lower median apparent modulus of 57 MPa. It failed in a brittle manner at a median stress of 1.7 MPa and strain level of 2.9%, demonstrating the structural importance of the collagen phase. This indicates why bone grafts prepared through cold-temperature processes are clinically favourable.