bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression of MACC1 and its role in the proliferation and apoptosis of salivary adenoid cystic carcinoma
Authors: Li Haifeng , Liao Xiaoying , Liu Yeqing , Shen Zhuojian , Gan Xiangfeng , Li Haigang , Huang Zhiquan 
Published: January 2015

BackgroundThe objective of this study was to investigate the relationship between metastasis‐associated in colon cancer‐1 and patient clinical characteristics. We also examined the role of metastasis‐associated in colon cancer‐1 in the proliferation and apoptosis in adenoid cystic carcinoma.Material and MethodsMetastasis‐associated in colon cancer‐1 expression was analysed in 65 paraffin‐embedded tissue specimens of salivary adenoid cystic carcinoma and 25 adjacent non‐cancerous tissues by immunohistochemistry (IHC). We used RNA interference technology to silence metastasis‐associated in colon cancer‐1 expression in ACCM cells. Cell Counting Kit‐8 tests, transwell experiments and flow cytometry were used to test the proliferation, cisplatin resistance, migration, invasion and apoptosis of ACCM cells.ResultsMetastasis‐associated in colon cancer‐1 nuclear and cytoplasmic expression in salivary adenoid cystic carcinoma tissue was higher than in the adjacent normal salivary tissue. The expression level was closely associated with tumour histological grading, perineural invasion and surrounding tumour invasion. The downregulation of metastasis‐associated in colon cancer‐1 expression inhibited proliferation and induced apoptosis in ACCM cells. The knock‐down of metastasis‐associated in colon cancer‐1 expression had no effect on migration, invasion and chemoresistance.ConclusionsMetastasis‐associated in colon cancer‐1 may have an important role in tumour development in adenoid cystic carcinoma. Metastasis‐associated in colon cancer‐1 is a potential biomarker for adenoid cystic carcinoma.
Triarchic Model of Psychopathy: Origins, Operationalizations, and Observed Linkages with Personality and General Psychopathology
Authors: Patrick Christopher J. , Drislane Laura E. 
Published: January 2015

AbstractThe triarchic model (Patrick, Fowles, & Krueger, 2009) was formulated to reconcile contrasting conceptions of psychopathy reflected in historic writings and contemporary assessment instruments, and to address persisting unresolved issues in the field. The model conceives of psychopathy as encompassing three distinct but interrelated phenotypic dispositions—disinhibition, boldness, and meanness—with biological referents. These dispositional constructs can be viewed as building blocks for alternative conceptions of psychopathy, and various existing psychopathy measures are presumed to index these constructs to differing degrees. This article summarizes the bases of the triarchic model in the conceptual and empirical literatures on psychopathy, and it describes linkages between the constructs of the model and established structural frameworks for personality and psychological disorders. Alternative methods for indexing the constructs of the model are described, and evidence regarding their interrelations and criterion‐related validity is reviewed. Promising aspects of the model for ongoing research on psychopathy are discussed, along with current gaps in knowledge/methods and recommended avenues for future research.
Genetic Analysis of Resistance Gene Analogues from a Sugarcane Cultivar Resistant to Red Rot Disease
Authors: Hameed Usman , Pan Yong‐Bao , Iqbal Javed 
Published: January 2015

AbstractOne of the important approaches for disease control in sugarcane is to develop a disease‐resistant variety; this may be accomplished through identification of resistance genes in sugarcane. In this study, PCR primers targeting the conserved motifs of the nucleotide‐binding site (NBS) class and kinase class of the resistance gene analogues (RGAs) were used to amplify the RGAs from a red rot‐resistant sugarcane cultivar (Saccharum spp. hybrid) HSF 240. Upon subcloning and sequencing, fifteen putative RGAs were identified. These RGAs shared 63–98% identity to the reported disease‐resistant genes in the NCBI GenBank database. Deduced amino acid sequences also showed the presence of expected conserved domains characteristic of RGAs. Phylogenetic analysis indicated that these RGAs clustered with R genes from other plant species. The findings will be useful for studying disease‐resistant genes in sugarcane.
Network science and oral health research
Authors: Maupome Gerardo , McCranie Ann 
Published: January 2015

AbstractThe present overview of research methods describes a scientific enquiry paradigm that is well established in other disciplines, including health research, but that is fairly new to oral health research. Social networks analysis (SNA) or network science research is a set of relational methods purporting to identify and characterize the connections between members of a system or network, as well as the structure of the network. Persons and communities making up the members of networks have commonly been the focus of SNA studies but corporations or living organisms might just as well be organized in networks. SNA is grounded in both graphic imagery and computational models. SNA is based on the assumptions that features and structure of networks are amenable to characterization, that such information sheds light on the ways members of the network relate to each other (sharing information, diseases, norms, and so on), and that through these connections between members the overall network structure and characteristics are shaped. The overview resorts to examples specific to oral health themes and proposes a few general avenues for population‐based research.
GGF2 Is Neuroprotective in a Rat Model of Cavernous Nerve Injury-Induced Erectile Dysfunction
Authors: Burnett Arthur L. , Sezen Sena F. , Hoke Ahmet , Caggiano Anthony O. , Iaci Jennifer , Lagoda Gwen , Musicki Biljana , Bella Anthony J. 
Published: January 2015

Abstract

Introduction
Erectile dysfunction is a major complication of radical prostatectomy, commonly associated with penile neuropathy. In animal models of peripheral nerve injury, glial growth factor-2 (GGF2), a member of the neuregulin family of growth factors, has neuroprotective and neurorestorative properties, but this potential has not been established after cavernous nerve (CN) injury.

Aims
The effectiveness of GGF2 in preserving axonal integrity and recovering erectile function in a rat model of radical prostatectomy-associated CN injury.

Methods
Adult male Sprague-Dawley rats underwent bilateral CN crush injury (BCNI) or sham surgery. Rats were administered GGF2 (0.5, 5, or 15 mg/kg) or vehicle subcutaneously 24 hour pre and 24-hour post-BCNI, and once weekly for 5 weeks. Erectile function was assessed in response to electrical stimulation of the CN. CN survival was assessed by fluorogold retrograde axonal tracing in major pelvic ganglia (MPG). Unmyelinated axons in the CNs were quantitated by electron microscopy.

Main Outcome Measures
Erectile function recovery, CN survival, and unmyelinated CN axon preservation in response to GGF2 treatment following BCNI.

Results
Erectile function was decreased (P &lt; 0.05) after BCNI, and it was improved (P &lt; 0.05) by all doses of GGF2. The number of fluorogold-labeled cells in the MPG was reduced (P &lt; 0.05) by BCNI and was increased (P &lt; 0.05) by GGF2 (0.5 and 5 mg/kg). The percentage of denervated Schwann cells in the BCNI group was higher (P &lt; 0.05) than that in the sham-treated group and was decreased (P &lt; 0.05) in the GGF2-treated (5 mg/kg) BCNI group. In the BCNI + GGF2 (5 mg/kg) group, the unmyelinated fiber histogram demonstrated a rightward shift, indicating an increased number of unmyelinated axons per Schwann cell compared with the BCNI group.

Conclusions
GGF2 promotes erectile function recovery following CN injury in conjunction with preserving unmyelinated CN fibers. Our findings suggest the clinical opportunity to develop GGF2 as a neuroprotective therapy for radical prostatectomy.
Foodfish Production Strategies Using 13‐cm and 19‐cm Hybrid Catfish, ♀Ictalurus punctatus ×  ♂Ictalurus furcatus
Authors: Rees Patrick , Engle Carole , Renukdas Nilima 
Published: January 2015

AbstractMost hybrid catfish, ♀Ictalurus punctatus × ♂Ictalurus furcatus, producers stock 18‐ to 19‐cm fingerlings in single‐batch production. While stocking smaller fingerlings would be less expensive, the economic trade‐offs of using smaller fingerlings is unknown. Two sizes of hybrid catfish fingerlings (13‐cm and 19‐cm) were stocked in single‐size treatments at 9884 head/ha and a multi‐size treatment with each size stocked at 9884 head/ha (total 19,768 head/ha). In the 13‐cm and 19‐cm single‐size treatments, 87 and 98%, respectively, reached market size in one season. In the multi‐size treatment, 77% of all fish stocked reached market size, demonstrating that more than half of the 13‐cm fish reached market size in this treatment. Gross, net, and marketable yields were significantly greater in the multi‐size treatment when compared to the 13‐cm or 19‐cm single‐size treatments, but were not significantly different between the two single‐size treatments. The 19‐cm single‐size treatment resulted in greater break‐even prices and economic risk than the other two treatments. This study demonstrated that 13‐cm hybrid catfish fingerlings can be raised economically in both single‐ and multi‐size production systems in the southern USA.
Apparent Protein and Energy Digestibility and Amino Acid Availability of Commercial Meat and Bone Meal for Nile Tilapia, Oreochromis niloticus
Authors: Xavier Tadeu O. , Michelato Mariana , Vidal Luiz V. O. , Furuya Valéria R. B. , Furuya Wilson M. 
Published: January 2015

AbstractMeat and bone meal (MBM) is an excellent source of amino acids (AAs) for fish but its proximate composition varies according to its origin and processing. Apparent digestibility coefficients (ADC) of dry matter, crude protein, and gross energy, and apparent availability coefficients (AAC) of essential and nonessential AAs (EAA and NEAA, respectively) in MBM containing various levels of crude protein (MBM‐340, 370, 400, 430, and 460 g/kg) were determined for juvenile Nile tilapia (Oreochromis niloticus, 32.7 ± 4.5 g). A reference diet containing 351.9 g/kg crude protein and 4541.1 kcal/kg of gross energy and test diets containing a 70:30 mixture of the reference diet and MBM were used with 5 g/kg Cr2O3 as an external indicator. ADC of dry matter, gross energy, and crude protein in MBM samples were 42.9–76.2%, 71.6–89.1%, and 49.4–86.9%, respectively. MBM‐400, 430, and 460 showed higher dry matter, gross energy, and crude protein ADC than that by MBM‐340 and 370. All AAs in MBM‐460 showed AAC of EAA > 90%, while the value for MBM‐430 was >85%, except arginine (82.1%) and lysine (84.8%). High ADC of energy, crude protein, and AAC in MBM‐430 and 460 indicate good potential as quantitative AA sources. Besides the low AA quality of MBM‐340, 370, and 400, their utilization is limited by low digestible energy and high ash, which should be considered in the production of least‐cost, well‐balanced, and sustainable diets for tilapia.
Spore Culture of Red Seaweed, Gloiopeltis furcata
Authors: Chen Suwen , Chen Lixiong , Zhu Changbo , Guo Yongjian , Guo Yihui 
Published: January 2015

AbstractTwo experiments were conducted to study Gloiopeltis furcata spore culture with different substrates in the nonreproduction season. Using spores discharged by fertile G. furcata frozen for storage, two experiments were conducted during the nonreproduction season. In the first experiment, G. furcata spores were collected and incubated in aquariums using palm line, polythene line, vinylon line, and three types of plastic sheets (white, ripple, and transparent polymethyl methacrylate) as substrates. In the second experiment, G. furcata spores were collected and incubated in a plastic tank using plastic sheets of different materials (PP, PE, and transparent PVC) and plastic even nets of different mesh sizes (3, 2, and 0.5 cm) as substrates. At the end of the experiment (after 60 d), discs with raised thallus were found on different plastic sheets and white plastic even nets, but no discs were visible on any type of line or on the black plastic even net (3‐cm mesh). The white plastic even net was found to be a suitable substrate for G. furcata spore culture in farms and for sporeling culture in sea. Results presented in this study are expected to aid in the progress of the artificial seeding of Gloiopeltis.
The Efficacy of a Novel Microbial 6‐Phytase Expressed in Aspergillus oryzae on the Performance and Phosphorus Utilization of Cold‐ and Warm‐Water Fish: Rainbow Trout, Oncorhynchus mykiss, and Nile Tilapia, Oreochromis niloticus
Authors: Verlhac‐Trichet Viviane , Vielma Jouni , Dias Jorge , Rema Paulo , Santigosa Ester , Wahli Thomas , Vogel Kurt 
Published: January 2015

AbstractThe efficacy and tolerance of a novel microbial 6‐phytase were investigated in rainbow trout, Oncorhynchus mykiss, and Nile tilapia, Oreochromis niloticus. Reference diets were sufficient in available phosphorus (P). The test diet limiting in available P was supplemented with phytase at 500, 1000, or 2000 phytase units/kg feed. The enzyme was effective in increasing total P apparent digestibility coefficient in relation to increasing the dose of phytase in rainbow trout and Nile tilapia. Zinc apparent digestibility improved in relation to phytase supplementation in rainbow trout. P release due to phytase supplementation ranged from 0.06 to 0.18% P/kg feed in rainbow trout and from 0.13 to 0.26% P/kg feed in Nile tilapia. A 58‐d performance trial was conducted to evaluate tolerance of fish to phytase supplementation. Dietary treatments consisted of a basal diet without phytase or supplemented with 2000 and 200,000 phytase units/kg feed. Results indicate that this novel microbial 6‐phytase is well tolerated by fish. Significant improvements for growth as well as feed conversion ratio were observed when the phytase was fed at 2000 phytase units/kg feed. This phytase is proven efficient in releasing P from phytate and could be added when plants are used for fish meal replacement in diets for salmonid and omnivorous fish.
Isotopic Evaluation of the Nutritional Contribution of Poultry By‐product Meal and Fish Meal to the Growth of Pacific White Shrimp, Litopenaeus vannamei
Authors: Gamboa‐Delgado Julián , Castañeda‐Solís Juan de Dios , Nieto‐López Martha G. , Villarreal‐Cavazos David , Cruz‐Suárez Lucía Elizabeth 
Published: January 2015

AbstractThe nutritional contribution of the dietary nitrogen supplied by poultry by‐product meal (PBM) and fish meal (FM) to the somatic growth of Pacific white shrimp, Litopenaeus vannamei, was assessed by means of stable isotope analysis. Seven experimental diets were formulated with different proportions of PBM replacing FM. Practical diets were formulated to replace 0, 35, 50, 65, 80, 95, and 100% of FM with PBM, on a dietary nitrogen basis. At the end of the experiment, there were no significant differences in survival among dietary treatments (89 ± 5%); however, significant differences in final wet weights were observed. Diets having FM replacement levels of 35 and 50% with PBM promoted mean final weights (708–789 mg) similar to those observed in shrimps fed on diet containing 100% FM (874 mg). Shrimp final mean weight significantly decreased as a function of PBM inclusion (r = −0.98) owing to the use of only two dietary nitrogen sources and by possible nutritional restrictions as PBM levels increased. The relative proportions of dietary nitrogen supplied by PBM and FM were incorporated in muscle tissue at proportions that were statistically similar to those established in the dietary formulations.
Effects of Dietary Protein and Lipid Levels on Growth, Nutrient Utilization, and the Whole‐body Composition of Turbot, Scophthalmus maximus, Linnaeus 1758, at Different Growth Stages
Authors: Liu Xingwang , Mai Kangsen , Liufu Zhiguo , Ai Qinghui 
Published: January 2015

AbstractThree 9‐wk feeding trials were performed to assess the effects of dietary protein and lipid levels on the growth, feed utilization, and body composition of turbot, Scophthalmus maximus Linnaeus, at three different growth stages with initial average weight 4.5 ± 0.01, 59.1 ± 0.24, and 209.1 ± 0.21 g, respectively. Six practical test diets were formulated to contain three protein levels (45, 50, and 55%), each of which was supplemented with two lipid levels (12.0 and 16.0%), to produce a range of P : E ratios (from 87.4 to 110.0 mg protein/kcal). The results of three experiments suggested that growth performance generally improved with increasing dietary protein irrespective of dietary lipid. Fish fed diets with 55% protein (12 and 16% lipid, P : E ratio of 110.9 and 107.1 mg protein/kcal) had the highest specific growth rates (SGRs), feed efficiency ratios (FERs), and energy retention. At the same protein level, FER and protein efficiency ratio (PER) increased significantly with the increase of lipid except in large fish (initial average weight 209.1 ± 0.21 g). SGR of medium turbot (initial average weight 59.1 ± 0.24 g) was also improved by the lipid increase, indicating a protein‐sparing effect of lipid.
Dietary Protein Requirement of Juvenile Dianchi Golden‐line Barbell, Sinocyclocheilus grahami
Authors: Deng Junming , Zhang Xi , Han Xuemei , Tao Linli , Bi Baoliang , Kang Bin 
Published: January 2015

AbstractA 10‐wk feeding trial was conducted to estimate the dietary protein requirements of juvenile Dianchi golden‐line barbell, Sinocyclocheilus grahami (initial average weight 7.55 g). Five isocaloric diets were formulated to contain graded levels of protein (29, 34, 39, 44, and 49%). Each diet was fed to triplicate groups of fish in a recirculating rearing system maintained at 18–22 C. Feed intake of fish fed the diet with 39% protein was significantly higher than those fed the diet with 29, 34, and 49% protein (P < 0.05). Weight gain, specific growth rate (SGR), and protein gain significantly increased with increasing dietary protein levels up to 39% (P < 0.05), whereas no significant differences were observed among fish fed the diet with 39, 44, and 49% protein (P > 0.05). In contrast, feed conversion ratio was significantly decreased with increasing dietary protein levels up to 39%. Maximum protein retention and protein efficiency ratio were observed in fish fed the diet with 39% protein. The regression analysis based on SGR and protein gain showed that the dietary protein requirements of juvenile S. grahami were 38.57% or 41.09% (equivalent to ca. 32.94% or 35.42% estimated digestible protein) of diet with a calculated digestible energy of 3.6 kcal/g.
Long‐term Growth Study of Male and Female Green Sea Urchins, Strongylocentrotus droebachiensis, under Constant Light and Temperature Regime
Authors: Siikavuopio Sten I. , James Philip , Sæther Bjørn‐Steinar 
Published: January 2015

AbstractJuvenile green sea urchins, Strongylocentrotus droebachiensis, with an initial mean test diameter of 9.5 mm (±1.2 SD) were held in the laboratory, in individual compartments and at constant temperatures (8.5 C) (n = 90) for a period of 99 wk. The animals were supplied with flow‐through sea water, and fed the Nofima sea urchin manufactured feed ad libitum. Growth, survival, feed intake, feed conversion ratio (FCR), and gonad index (GI) of the sea urchins were monitored during this period. The mortality was 11% throughout the experimental period. The test diameter (TD) increased significantly with time, best described by a polynomial equation (TD = 0.8756 + 0.584Time − 0.002Time2, R2 = 0.995). Sex was included as dummy variable, but did not contribute significantly; hence, there were no differences in growth trajectories between males and females. The study showed there were no significant differences in body wet weight or FCR between males and females. However at the end of the experiment the female urchins had a significantly higher GI of 31.1% (±4.4), compared with the male urchins which had an average GI of 26.8% (±5.4). In conclusion, the present study showed that male and female green sea urchins have the same growth capacity and feed convention ratio but by the end of the experiment the gonad index was significantly higher in female than male urchins.
The Social Life of Health Insurance in Low‐ to Middle‐income Countries: An Anthropological Research Agenda
Authors: Dao Amy , Nichter Mark 
Published: January 2015

The following article identifies new areas for engaged medical anthropological research on health insurance in low‐ and middle‐income countries (LMICs). Based on a review of the literature and pilot research, we identify gaps in how insurance is understood, administered, used, and abused. We provide a historical overview of insurance as an emerging global health panacea and then offer brief assessments of three high‐profile attempts to provide universal health coverage. Considerable research on health insurance in LMICs has been quantitative and focused on a limited set of outcomes. To advance the field, we identify eight productive areas for future ethnographic research that will add depth to our understanding of the social life and impact of health insurance in LMICs. Anthropologists can provide unique insights into shifting health and financial practices that accompany insurance coverage, while documenting insurance programs as they evolve and respond to contingencies.
Relationship between invasion success and colony breeding structure in a subterranean termite
Authors: Perdereau E. , Bagnères A.‐G. , Vargo E.L. , Baudouin G. , Xu Y. , Labadie P. , Dupont S. , Dedeine F. 
Published: January 2015

AbstractFactors promoting the establishment and colonization success of introduced populations in new environments constitute an important issue in biological invasions. In this context, the respective role of pre‐adaptation and evolutionary changes during the invasion process is a key question that requires particular attention. This study compared the colony breeding structure (i.e. number and relatedness among reproductives within colonies) in native and introduced populations of the subterranean pest termite, Reticulitermes flavipes. We generated and analysed a data set of both microsatellite and mtDNA loci on termite samples collected in three introduced populations, one in France and two in Chile, and in the putative source population of French and Chilean infestations that has recently been identified in New Orleans, LA. We also provided a synthesis combining our results with those of previous studies to obtain a global picture of the variation in breeding structure in this species. Whereas most native US populations are mainly composed of colonies headed by monogamous pairs of primary reproductives, all introduced populations exhibit a particular colony breeding structure that is characterized by hundreds of inbreeding reproductives (neotenics) and by a propensity of colonies to fuse, a pattern shared uniquely with the population of New Orleans. These characteristics are comparable to those of many invasive ants and are discussed to play an important role during the invasion process. Our finding that the New Orleans population exhibits the same breeding structure as its related introduced populations suggests that this native population is pre‐adapted to invade new ranges.