bluuebunny/crossref_metadata_2025_split huggingface dataset:
One Step Deposition of Sol‐Gel Carbon Nanotubes Biocomposite for Reagentless Electrochemical Devices
Authors: Urbanová Veronika , Etienne Mathieu , Walcarius Alain 
Published: November 2012

AbstractA simple way to immobilization of vitamin K3 by physical adsorption onto the surface of single‐walled carbon nanotubes (CNT‐VK3) and the implementation of these functionalized carbon nanotubes into a reagentless electrochemical devices based on biodoped silica sol‐gel film are described. A sol‐gel composite thin film containing glucose dehydrogenase, diaphorase, cofactor and CNT‐VK3 was either drop‐coated or electrodeposited onto a glassy carbon electrode surface. Sol‐gel/CNT‐VK3/cofactor/glucose dehydrogenase/diaphorase composite modified electrodes showed effective catalysis towards glucose oxidation in both cases.
Carbon Nanotube Based Sensor for Simultaneous Determination of Acetaminophen and Ascorbic Acid Exploiting Multiple Response Optimization and Measures in the Presence of Surfactant
Authors: Duarte Eduardo Henrique , Kubota Lauro Tatsuo , Tarley César Ricardo Teixeira 
Published: November 2012

AbstractA simple procedure for the simultaneous determination of acetaminophen (AC) and ascorbic acid (AA) by differential pulse voltammetry (DPV) using a carbon nanotube paste electrode exploiting measures in cetylpyridinium bromide (CPB) medium is described. Under the best instrumental parameters of DPV, optimized by means of factorial design, the calibration plots in the range 100.0–700.0 µmol L−1 (r=0.993) and 39.4–146.3 µmol L−1 (r=0.995) with limits of detection of 7.1 and 2.1 µmol L−1, were achieved for AA and AC, respectively. The developed method was successfully applied for the AC and AA determination in pharmaceutical formulations, whose accuracy was attested by comparison with HPLC method.
Evaluation of a standardized humor group in a clinical setting: a feasibility study for older patients with depression
Authors: Konradt Brigitte , Hirsch Rolf D. , Jonitz Markus F. , Junglas Karin 
Published: November 2012

BackgroundPositive effects of humor on older patients with depressive symptoms have been repeatedly reported. Empirical evidence however is rare. We investigated the efficacy of a standardized humor therapy group in a clinical context especially for older depressed patients.Patients and methodsAn experimental group with treatment (49 patients) was compared with a control group with no treatment (50 patients) in a semi‐randomized design. Included were patients with major depression according to ICD‐10. A set of questionnaires (Geriatric Depression Scale, Short Form Health Survey, State‐Trait‐Cheerfulness Inventory, and Satisfaction with Life Scale) was administered pretreatment and posttreatment.ResultsBoth groups showed improvement for depression, suicidal tendency, state cheerfulness, and state bad mood. Only participants of the humor group showed changes of state seriousness and satisfaction with life (p < 0.05). Further trends could be demonstrated for higher changes in state cheerfulness and resilience for the humor group.ConclusionOur results indicate an additional benefit of this specific therapeutic intervention for older depressed patients. Copyright © 2012 John Wiley & Sons, Ltd.
Silica Boron Sulfuric Acid Nanoparticles: As an Efficient and Reusable Catalyst for the Large‐Scale Synthesis of α‐Amino Nitriles Using the Strecker Reaction
Authors: Khalafi‐Nezhad Ali , Foroughi Habib Ollah , Panahi Farhad 
Published: November 2012

ABSTRACTA highly efficient and green procedure for the large‐scale synthesis of α‐amino nitriles using three‐component condensation of carbonyl compounds, amines, and trimethylsilylcyanide has been developed. Silica boron sulfuric acid nanoparticles (SBSANs) are found as an efficient heterogeneous catalyst for the promotion of this process at room temperature under solvent‐free conditions. This protocol offers an effective and scale‐up procedure for the synthesis of various α‐amino nitriles using a wide range of amines and carbonyl compounds in relatively short reaction time with the excellent isolated yields. In addition, the SBSAN catalyst is easily separated from the reaction mixture by simple filtration and can be reused several times. © 2012 Wiley Periodicals, Inc. Heteroatom Chem 24:1–8, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.21052
Numerical simulation of mixed convection in a porous medium filled with water/Al2 O3 nanofluid
Authors: Mittal Nitesh , Manoj Vijay , Kumar D. Santhosh , Satheesh A. 
Published: November 2012

AbstractThe present work encloses the application of a Brinkman–extended Darcy model in a problem concerning mixed convection ina lid–driven porous cavity using nanofluids. The transport equations are solved numerically by the finite volume method on a co–located grid arrangement using the Quadratic Upstream Interpolation for Convective Kinematics (QUICK) scheme. The effects of governing parameters, namely, Grashof number (Gr), Darcy number (Da), and solid volume fraction $(\chi )$, on the streamlines and the isotherms are studied. The present results are validated by favorable comparisons with previously published results and are in good agreement with them. The present numerical results show that the addition of nanoparticles to a base fluid has produced an augmentation of the heat transfer coefficient and it is found to increase significantly with an increase of the particle volume concentration. It is observed from the results that at the higher value of the Grashof number (Gr = 104 ), the average Nusselt number increases with an increase in the Darcy number for a constant solid volume fraction. The detailed results are reported by means of streamlines, isotherms, and Nusselt numbers. © 2012 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.21029
PITX2: A promising predictive biomarker of patients' prognosis and chemoradioresistance in esophageal squamous cell carcinoma
Authors: Zhang Jia‐Xing , Tong Zhu‐Ting , Yang Lin , Wang Fan , Chai Hui‐Ping , Zhang Fan , Xie Ming‐Ran , Zhang An‐Li , Wu Li‐Ming , Hong Hao , Yin Lv , Wang Hao , Wang Hong‐Yan , Zhao Yuan 
Published: November 2012

AbstractThe paired‐like homeodomain transcription factor 2 (PITX2), a downstream effector of wnt/β‐catenin signaling, is well known to play critical role during normal embryonic development. However, the possible involvement of PITX2 in human tumorigenesis remains unclear. In this study, we extend its function in human esophageal squamous cell carcinoma (ESCC). The real‐time PCR, Western blotting and immunohistochemistry (IHC) methods were applied to examine expression pattern of PITX2 in two different cohorts of ESCC cases treated with definitive chemoradiotherapy (CRT). Receiver operating characteristic (ROC) curve analysis was used to determine the cutoff point for PITX2 high expression in the training cohort. The ROC‐derived cutoff point was then subjected to analyze the association of PITX2 expression with patients' survival and clinical characteristics in training and validation cohort, respectively. The expression level of PITX2 was significantly higher in ESCCs than that in normal esophageal mucosa. There was a positive correlation between PITX2 expression and clinical aggressiveness of ESCC. Importantly, high expression of PITX2 was observed more frequently in CRT resistant group than that in CRT effective group (p < 0.05). Furthermore, high expression of PITX2 was associated with poor disease‐specific survival (p < 0.05) in ESCC. Then, the MTS, clonogenic survival fraction and cell apoptosis experiments showed that knockdown of PITX2 substantially increased ESCC cells sensitivity to ionizing radiation (IR) or cisplatin in vitro. Thus, the expression of PITX2, as detected by IHC, may be a useful tool for predicting CRT resistance and serves as an independent molecular marker for poor prognosis of ESCC patients treated with definite CRT.
Clinical evidence of parietal cortex dysfunction and correlation with extent of allodynia in CRPS type 1
Authors: Cohen H. , McCabe C. , Harris N. , Hall J. , Lewis J. , Blake D.R. 
Published: November 2012

AbstractBackgroundUnusual symptoms such as digit misidentification and neglect‐like phenomena have been reported in complex regional pain syndrome (CRPS), which we hypothesized could be explained by parietal lobe dysfunction.MethodsTwenty‐two patients with chronic CRPS attending an in‐patient rehabilitation programme underwent standard neurological examination followed by clinical assessment of parietal lobe function and detailed sensory testing.ResultsFifteen (68%) patients had evidence of parietal lobe dysfunction. Six (27%) subjects failed six or more test categories and demonstrated new clinical signs consistent with their parietal testing impairments, which were impacting significantly on activities of daily living. A higher incidence was noted in subjects with >1 limb involvement, CRPS affecting the dominant side and in left‐handed subjects.Eighteen patients (82%) had mechanical allodynia covering 3–57.5% of the body surface area. Allochiria (unilateral tactile stimulation perceived only in the analogous location on the opposite limb), sensory extinction (concurrent bilateral tactile stimulation perceived only in one limb), referred sensations (unilateral tactile stimulation perceived concurrently in another discrete body area) and dysynchiria (unilateral non‐noxious tactile stimulation perceived bilaterally as noxious) were present in some patients. Greater extent of body surface allodynia was correlated with worse parietal function (Spearman's rho = −0.674, p = 0.001).ConclusionIn patients with chronic CRPS, detailed clinical examination may reveal parietal dysfunction, with severity relating to the extent of allodynia.
Experimental myalgia induced by repeated infusion of acidic saline into the human masseter muscle does not cause the release of algesic substances
Authors: Ernberg M. , Castrillon E.E. , Ghafouri B. , Larsson B. , Gerdle B. , List T. , Svensson P. 
Published: November 2012

AbstractBackgroundAnimal studies have shown that two repeated intramuscular injections of acidic saline induce mechanical allodynia that lasts for 4 weeks with spread to the contralateral side. In this study, we tested the hypothesis that two repeated intramuscular infusions of acidic saline into the human masseter muscle is associated with pain, mechanical allodynia and release of algesic substances. Eighteen healthy volunteers participated. On day 1, 2.5 mL of acidic saline (pH 3.3) was infused into one of the masseter muscles and isotonic saline (pH 6.0) into the other (randomized and single‐blind). Two days later, intramuscular microdialysis was performed to sample serotonin, glutamate, pyruvate, lactate and glucose, during which the saline infusions were repeated. Pain and pressure pain thresholds (PPTs) were recorded before and after infusions on both days.ResultsPain intensity induced by the infusions was higher after acidic than that after isotonic saline (p < 0.05). PPTs were decreased on both sides after microdialysis compared with baseline day 1 (p's < 0.05), but there were no differences in PPTs between sides at any time point. The levels of serotonin, glutamate, pyruvate, lactate or glucose did not change significantly during microdialysis.ConclusionInfusion of acidic saline caused low levels of muscle pain, but no mechanical allodynia and no increased release of algesic substances. The value of this model appears modest, but future studies could be performed with larger sample size and higher flow rate before definite conclusions about the validity of the model for craniofacial myalgia can be drawn.
Time‐dependent release of growth factors from implant surfaces treated with plasma rich in growth factors
Authors: Sánchez‐Ilárduya María Belén , Trouche Elodie , Tejero Ricardo , Orive Gorka , Reviakine Ilya , Anitua Eduardo 
Published: November 2012

AbstractPlasma rich in growth factors (PRGFs) technology is an autologous platelet‐rich plasma approach that provides a pool of growth factors and cytokines that have been shown to increase tissue regeneration and accelerate dental implant osseointegration. In this framework, the spatiotemporal release of growth factors and the establishment of a provisional fibrin matrix are likely to be key aspects governing the stimulation of the early phases of tissue regeneration around implants. We investigated the kinetics of growth factor release at implant surfaces functionalized either with PRGFs or platelet‐poor plasma and correlated the results obtained with the morphology of the resulting interfaces. Our main finding is that activation and clot formation favors longer residence times of the growth factors at the interfaces studied, probably due to their retention in the adsorbed fibrin matrix. The concentration of the platelet‐derived growth factors above the interfaces becomes negligible after 2–4 days and is significantly higher in the case of activated interfaces than in the case of nonactivated ones, whereas that of the plasmatic hepatocyte growth factor is independent of platelet concentration and activation, and remains significant for up to 9 days. Platelet‐rich plasma preparations should be activated to permit growth factor release and thereby facilitate implant surface osseointegration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013.
Measuring Congruence of Affective Images of Destinations and Their Slogans
Authors: Lehto Xinran Y. , Lee Gwangjin , Ismail Joseph 
Published: November 2012

ABSTRACTThis study examines whether consumers' emotive responses to a destination slogan are consistent with their reactions to the destination that the slogan is created for and whether affective image congruence between the two contributes to destination brand awareness. Findings conclude that destinations and their slogans may not evoke similar emotions and that affective similarity between a destination and its slogan significantly improves consumers' ability to correctly identify a destination from its slogan. This study proposes and tests a practical tool to aid destination marketers in their effort to evaluate the effectiveness of their brand slogans. Copyright © 2012 John Wiley & Sons, Ltd.
Sonographic evaluation of vascular pulmonary reactivity following oxygen administration in fetuses with normal lung development
Authors: DeKoninck Philip , Lewi Paul , Done Elisa , Richter Jute , Gucciardo Léonardo , Mieghem Tim Van , Deprest Jan A. 
Published: November 2012

ABSTRACTObjectiveThis study aimed to establish nomograms for sonographic assessment of fetal pulmonary vascular reactivity following maternal hyperoxygenation.Study designSixty‐two healthy fetuses were assessed at four weekly intervals from 26 weeks onwards. Pulmonary reactivity was evaluated using Doppler ultrasound in the main pulmonary artery and in the first branch of this main pulmonary artery. The difference in pulsatility index (∆PI) during maternal inhalation of a mixture of room air and oxygen (9 L/min) for at least 10 min was expressed as a percentage. Nomograms were constructed, and Kaplan–Meier curves were used to express the occurrence of a reactive test (∆PI ≥ 20%) with advancing gestation.ResultsIn the first branch, linear regression analysis revealed a significant correlation of ∆PI (%) with gestational age (r2 = 0.04, p = 0.0057). Large inter‐individual and intra‐individual variability was noted. The ∆PI (%) in the main pulmonary artery remained constant throughout gestation (6.62 ± 17.83%).ConclusionVascular reactivity in the pulmonary circulation increases in the first branch of the pulmonary artery. Large individual variability is limiting its use as a management tool. © 2012 John Wiley & Sons, Ltd.
Development and Performance of the W/Sb2O3/KIO4/Lubricant Pyrotechnic Delay in the US Army Hand‐Held Signal
Authors: Poret Jay C. , Shaw Anthony P. , Csernica Christopher M. , Oyler Karl D. , Estes Deven P. 
Published: November 2012

AbstractGassy pyrotechnic delays composed of tungsten, antimony(III) oxide, potassium periodate, and various lubricants have been developed for use in the US Army hand‐held signal. The new compositions were developed to replace the current formulation, which contains potassium perchlorate and barium chromate, chemicals that are facing increasing scrutiny due to environmental regulation. The hand‐held signal delay housing was used to demonstrate the burning rate tunability of the new compositions. The addition of 1–5 % of waxy lubricants (stearic acid or calcium stearate) was found to have a profound effect on burning rate. The effect of tungsten content and the Sb2O3/KIO4 ratio on burning rate was also probed. A wide range of inverse burning rates (2 to 15 s cm−1) were demonstrated, which encompasses the 7 to 8.5 s cm−1 range required by the hand‐held signal. The W/KIO4 reaction produces I2, which was observed by visible spectroscopy in the vapor above a sample of combustion residue.
IKE vulnerability discovery based on fuzzing
Authors: Yang Huan , Zhang Yuqing , Hu Yu‐pu , Liu Qi‐xu 
Published: November 2012

ABSTRACTInternet Key Exchange (IKE) protocol is widely applied on the Internet to protect confidentiality of the Internet communication. However, there are many high‐risk security vulnerabilities in various IKE implementations. Traditional fuzzing approaches with the aim of discovering vulnerabilities have some blind spots, such as time‐consuming, low efficiency, and low degree of automation. This paper introduces a new vulnerability discovering approach based on fuzzing and applies the approach to the IKE protocol. Through summarizing the most comprehensive vulnerable points of IKE protocol and proposing a two‐stage test cases library, an IKE protocol vulnerability discovering tool called IKEProFuzzer is designed and implemented. It is a network protocol fuzzing framework with extensibility and automated Monitor/Debugger designed by ourselves. In the experiments, IKEProFuzzer has discovered 14 vulnerabilities, including nine released vulnerabilities and five unreleased ones, which affect many kinds of routers and applications. The evaluation results prove the feasibility, efficiency, and extensibility of the approach compared with the existing approaches. Copyright © 2012 John Wiley & Sons, Ltd.
Brief Report: Self-Organizing Neuroepithelium from Human Pluripotent Stem Cells Facilitates Derivation of Photoreceptors
Authors: Boucherie Cédric , Mukherjee Sayandip , Henckaerts Els , Thrasher Adrian J. , Sowden Jane C. , Ali Robin R. 
Published: November 2012

Abstract
Retinitis pigmentosa, other inherited retinal diseases, and age-related macular degeneration lead to untreatable blindness because of the loss of photoreceptors. We have recently shown that transplantation of mouse photoreceptors can result in improved vision. It is therefore timely to develop protocols for efficient derivation of photoreceptors from human pluripotent stem (hPS) cells. Current methods for photoreceptor derivation from hPS cells require long periods of culture and are rather inefficient. Here, we report that formation of a transient self-organized neuroepithelium from human embryonic stem cells cultured together with extracellular matrix is sufficient to induce a rapid conversion into retinal progenitors in 5 days. These retinal progenitors have the ability to differentiate very efficiently into Crx+ photoreceptor precursors after only 10 days and subsequently acquire rod photoreceptor identity within 4 weeks. Directed differentiation into photoreceptors using this protocol is also possible with human-induced pluripotent stem (hiPS) cells, facilitating the use of patient-specific hiPS cell lines for regenerative medicine and disease modeling.
Gata4 Blocks Somatic Cell Reprogramming By Directly Repressing Nanog
Authors: Serrano Felipe , Calatayud Carles F. , Blazquez Marina , Torres Josema , Castell Jose V. , Bort Roque 
Published: November 2012

Abstract
Somatic cells can be reprogrammed to induced pluripotent stem (iPS) cells by ectopic expression of the four factors Oct4, Klf4, Sox2, and Myc. Here, we investigated the role of Gata4 in the reprogramming process and present evidence for a negative role of this family of transcription factors in the induction of pluripotency. Coexpression of Gata4 with Oct4, Klf4, and Sox2 with or without Myc in mouse embryonic fibroblasts greatly impaired reprogramming and endogenous Nanog expression. The lack of Nanog upregulation was associated with a blockade in the transition from the initiation phase of reprogramming to the full pluripotent state characteristic of iPS cells. Addition of Nanog to the reprogramming cocktail blocked the deleterious effects observed with Gata4 expression. Downregulation of endogenous Gata4 by short hairpin RNAs during reprogramming both accelerated and increased the efficiency of the process and augmented the mRNA levels of endogenous Nanog. Using comparative genomics, we identified a consensus binding site for Gata factors in an evolutionary conserved region located 9 kb upstream of the Nanog gene. Using chromatin immunoprecipitation, gel retardation, and luciferase assays, we found that Gata4 bound to this region and inhibited Nanog transcription in mouse embryonic stem cells. Overall, our results describe for first time the negative effect of Gata4 in the reprogramming of somatic cells and highlight the role of Gata factors in the transcriptional networks that control cell lineage choices in the early embryo.
Effective Elimination of Cancer Stem Cells By a Novel Drug Combination Strategy
Authors: Yuan Shuqiang , Wang Feng , Chen Gang , Zhang Hui , Feng Li , Wang Lei , Colman Howard , Keating Michael J. , Li Xiaonan , Xu Rui-Hua , Wang Jianping , Huang Peng 
Published: November 2012

AbstractDevelopment of effective therapeutic strategies to eliminate cancer stem cells, which play a major role in drug resistance and disease recurrence, is critical to improve cancer treatment outcomes. Our study showed that glioblastoma stem cells (GSCs) exhibited low mitochondrial respiration and high glycolytic activity. These GSCs were highly resistant to standard drugs such as carmustine and temozolomide (TMZ), but showed high sensitivity to a glycolytic inhibitor 3-bromo-2-oxopropionate-1-propyl ester (3-BrOP), especially under hypoxic conditions. We further showed that combination of 3-BrOP with carmustine but not with TMZ achieved a striking synergistic effect and effectively killed GSCs through a rapid depletion of cellular ATP and inhibition of carmustine-induced DNA repair. This drug combination significantly impaired the sphere-forming ability of GSCs in vitro and tumor formation in vivo, leading to increase in the overall survival of mice bearing orthotopic inoculation of GSCs. Further mechanistic study showed that 3-BrOP and carmustine inhibited glyceraldehyde-3-phosphate dehydrogenase and caused a severe energy crisis in GSCs. Our study suggests that GSCs are highly glycolytic and that certain drug combination strategies can be used to effectively overcome their drug resistance based on their metabolic properties.
Characteristics of the compact plasma device AIT‐PID with multicusp magnetic confinement
Authors: Takamura Shuichi 
Published: November 2012

AbstractA unique plasma‐generation device called the “Aichi Institute of Technology‐Plasma Irradiation Device” has been developed for plasma–wall interaction (PWI) studies toward realization of a fusion reactor. It has power‐saving characteristics as well as compactness due to the employment of a multicusp magnetic field configuration instead of a strong axial magnetic field. Helium as well as argon plasmas are generated, showing an outstanding property of production of the hot‐electron component which can be controlled by changing the working gas pressure. Utilizing such outstanding properties, some typical studies on PWI are introduced. An azimuthally asymmetric confinement depending on the direction of magnetic field lines, particularly for energetic electrons, has been found. Some discussions on the physical mechanisms are given. IEEJ Trans 2011 DOI: 10.1002/tee.21801