bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of Modification of the 3M™ Molecular Detection Assay (MDA) Salmonella Method (2013.09) for the Detection of Salmonella in Selected Foods: Collaborative Study
Authors: Bird Patrick , Fisher Kiel , Boyle Megan , Huffman Travis , Benzinger M Joseph , Bedinghaus Paige , Flannery Jonathon , Crowley Erin , Agin James , Goins David , Benesh DeAnn , David John ,   Collaborators:, Awad D , Bandu M , Blanchard K , Bosco D , Brooks R , Clark D , Dammann H , Dyszel J , Gill V , Greenwell M , Gwinn C , Horan M , Jurgens J , Kelly M , Lewis D , Luce S , Marchent J , McMahon W , Mello I , Montez S , Moosekian S , Morey A , Newman K , Oltman M , Ontiberos M , Rajkowski K , Ruebl J , Stawick B , Thompson L , Vross M 
Published: September 2014

Abstract
The 3M™ Molecular Detection Assay (MDA) Salmonella utilizes isothermal amplification of nucleic acid sequences with high specificity, efficiency, rapidity and bioluminescence to detect amplification of Salmonella spp. in food, food-related, and environmental samples after enrichment. A method modification and matrix extension study of the previously approved AOAC Official MethodSM 2013.09 was conducted, and approval of the modification was received on March 20, 2014. Using an unpaired study design in a multilaboratory collaborative study, the 3M MDA Salmonella method was compared to the U.S. Department of Agriculture/Food Safety and Inspection Service (USDA/FSIS) Microbiology Laboratory Guidebook (MLG) 4.05 (2011), Isolation and Identification of Salmonella from Meat, Poultry, Pasteurized Egg, and Catfish Products for raw ground beef and the U.S. Food and Drug Administration (FDA)/Bacteriological Analytical Manual (BAM) Chapter 5, Salmonella reference method for wet dog food following the current AOAC guidelines. A total of 20 laboratories participated. For the 3M MDA Salmonella method, raw ground beef was analyzed using 25 g test portions, and wet dog food was analyzed using 375 g test portions. For the reference methods, 25 g test portions of each matrix were analyzed. Each matrix was artificially contaminated with Salmonella at three inoculation levels: an uninoculated control level (0 CFU/test portion), a low inoculum level (0.2–2 CFU/test portion), and a high inoculum level (2–5 CFU/test portion). In this study, 1512 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD). For the low-level raw ground beef test portions, the following dLPOD (difference between the LPODs of the reference and candidate method) values with 95% confidence intervals were obtained: –0.01 (–0.14, +0.12). For the low-level wet dog food test portions, the following dLPOD with 95% confidence intervals were obtained: –0.04 (–0.16, +0.09). No significant differences were observed in the number of positive samples detected by the 3M MDA Salmonella method versus either the USDA/FSIS-MLG or FDA/BAM methods.
Determination of Flavanols and Procyanidins (DP 1–10) in Cocoa-Based Ingredients and Products by UHPLC: First Action 2013.03
Authors: Machonis Philip R , Jones Matthew A , Kwik-Uribe Catherine , Dowell Dawn 
Published: September 2014

Abstract
Single-laboratory validation data were reviewed by the Expert Review Panel (ERP) of the Stakeholder Panel on Strategic Food Analytical Methods at the AOAC Mid-Year Meeting, March 12–14, 2013, in Rockville, MD. The ERP determined that the data presented met established standard method performance requirements and adopted a method for determination of flavanols and procyanidins (DP 1–10) in cocoa-based ingredients and products by ultra-HPLC as AOAC Official First Action Method 2013.03 on March 14, 2013. The flavanols and procyanidins (DP 1–10) are eluted using a binary gradient (solvents A and B) consisting of 98 + 2 (v/v) acetonitrile–glacial acetic acid (A) and 95 + 3 + 2 (v/v/v) methanol–water–glacial acetic acid (B). The mobile phase is applied to a diol stationary phase. Detection occurs using fluorescence detection. Recovery of flavanols and procyanidins (DP 1–10) from both high- and low-fat matrixes was 98.4–99.8%. Precision was determined for seven different sample types (cocoa extract, cocoa nib, natural cocoa powder, cocoa liquor, alkalized cocoa powder, dark chocolate, and milk chocolate).
Phytochemical Fingerprints of Lime Honey Collected in Serbia
Authors: Gašić Uroš , Šikoparija Branko , Tosti Tomislav , Trifković Jelena , Milojković-Opsenica Dušanka , Natić Maja , Tešić Živoslav 
Published: September 2014

Abstract
Composition of phenolic compounds and the sugar content were determined as the basis for characterization of lime honey from Serbia. Particular attention was given to differences in phytochemical profiles of ripe and unripe lime honey and lime tree nectar. Melissopalynological analysis confirmed domination of Tilia nectar in all analyzed samples. Phenolic acids, abscisic acid, flavonoids, and flavonoid glycosides were determined by means of ultra-HPLC coupled with a hybrid mass spectrometer (UHPLC–OrbiTrap). Sugar content was determined using high-performance anion-exchange chromatography with amperometric detection. Similar phenolic compounds characterized unripe and ripe honeys, while the lime tree nectar profile showed notable differences. Compared to lime tree nectar, a high amount of chrysin, pinocembrin, and galangin were detected in both ripe and unripe lime honey. Fructose and glucose were the major constituents of all investigated samples, and amounts were within the limits established by European Union legislation. Sucrose content in the nectar sample was up to two-fold higher when compared to all honey samples. Isomaltose and gentiobiose with turanose content were different in analyzed production stages of lime honey.
Optimization of Extraction Based on the Thin-Layer Chromatographic Fingerprints of Common Thyme
Authors: Orłowska Marta , Stanimirova Ivana , Staszek Dorota , Sajewicz Mieczysław , Kowalska Teresa , Waksmundzka-Hajnos Monika 
Published: September 2014

Abstract
The main goal of this study was to establish the optimum accelerated solvent extraction (ASE) conditions for the isolation of the total phenolics contained in common thyme (Thymus vulgaris L.) based on the results of TLC. Additionally, two different extraction methods, i.e., Soxhlet extraction and ASE, were compared in terms of their relative performance. Binary methanol–water mixtures in different volume proportions were used as the extraction solvents. Thirteen experiments utilizing different methanol concentrations and different extraction temperatures were designed using response surface methodology. Additionally, the temperature dependence of rosmarinic and caffeic acid extraction yields was analyzed by applying HPLC. Finally, the optimum working conditions established within the framework of the assumed central composite experimental design were determined to be 27% methanol at 130°C. It was found that the extraction carried out by ASE provided higher total phenolic yields than that done in the Soxhlet apparatus. The final aim of this study was a comparison of the six different Thymus species in terms of their total phenolic content and their TLC fingerprints. It was found that the phenolic content of four species, T. pulegioides, T. kosteleckyanus, T. citriodorus, and T. marschallianus, differs from the phenolic content of T. vulgaris and T. serpyllum.
Serum Concentrations of Prostate-Specific Antigen Measured Using Immune Extraction, Trypsin Digestion, and Tandem Mass Spectrometry Quantification of LSEPAELTDAVK Peptide
Authors: Klee Eric W. , Bondar Olga P. , Goodmanson Marcia K. , Trushin Sergey A. , Bergstralh Eric J. , Singh Ravinder J. , Anderson N. Leigh , Klee George G. 
Published: September 2014

Context.—Prostate-specific antigen (PSA) is a 34-kDa glycoprotein with chymotrypsin-like enzyme activity that circulates both in free forms and complexed to various enzyme inhibitors including antichymotrypsin and α2-macroglobulin. Prostate-specific antigen bound to α2-macroglobulin is not detected by commercial PSA immunoassays.
Objective.—To develop a mass spectrometry assay that detects the same forms of PSA as the immunoassays, which could serve as a reference for harmonizing PSA immunoassays.
Design.—Prostate-specific antigen was immune extracted from serum, trypsin was digested, and the LSEPAELTDAVK peptide was quantitated on an API 5000 spectrometer. Calibrators were made by adding 10% free and 90% antichymotrypsin-bound PSA to female sera. The assay was standardized to the World Health Organization 96/670 reference standard. Validation of clinical utility and comparisons with 2 immunoassays (Roche cobas and Beckman Access) were performed using frozen sera aliquots from 100 men undergoing prostate biopsy (50 negative, 50 with cancer) and 5 serial samples collected over time from 5 men with advanced prostate cancer.
Results.—The antibody extraction efficiency was greater than 99%. The assay has an analytic range from 1.2 to 76 ng/mL, with precision ranging from 8.6% at 1.5 ng/mL to 5.4% at 27 ng/mL. The mass spectrometry assay correlated well with 2 immunoassays. All 3 assays showed statistically equivalent separation of prostate cancer from benign disease using receiver operating characteristic curve analysis.
Conclusions.—This mass spectrometry assay can reliably measure PSA concentrations in human serum and could serve as a reference standard for harmonizing PSA immunoassays.
Proximal Tubulopathies Associated With Monoclonal Light Chains: The Spectrum of Clinicopathologic Manifestations and Molecular Pathogenesis
Authors: Herrera Guillermo A. 
Published: September 2014

Context.—Lesions associated with monoclonal light and heavy chains display a variety of glomerular, tubular interstitial, and vascular manifestations. While some of the entities are well recognized, including light and heavy chain deposition diseases, AL (light chain) and AH (heavy chain) amyloidosis, and light chain (“myeloma”) cast nephropathy, other lesions centered on proximal tubules are much less accurately identified, properly diagnosed, and adequately understood in terms of pathogenesis and molecular mechanisms involved. These proximal tubule–centered lesions are typically associated with monoclonal light chains and have not been reported in patients with circulating monoclonal heavy chains.Objective.—To determine the incidence of proximal tubulopathies in a series of patients with monoclonal light chain–related renal lesions and characterize them with an emphasis on clinical correlations and elucidation of molecular mechanisms involved in their pathogenesis.Design.—A study of 5410 renal biopsies with careful evaluation of light microscopic, immunofluorescence, and electron microscopic findings was conducted to identify these monoclonal light/heavy chain–related lesions. In selected cases, ultrastructural immunolabeling was performed to better illustrate and understand molecular mechanisms involved or to resolve specific diagnostic difficulties.Results.—In all, 2.5% of the biopsies were diagnosed as demonstrating renal pathology associated with monoclonal light or heavy chains. Of these, approximately 46% were classified as proximal tubule–centered lesions, also referred to as monoclonal light chain–associated proximal tubulopathies. These proximal tubulopathies were divided into 4 groups defined by characteristic immunomorphologic manifestations associated with specific clinical settings.Conclusions.—These are important lesions whose recognition in the different clinical settings is extremely important for patients' clinical management, therapeutic purposes, and prognosis. These entities have been segregated into 4 distinct variants, conceptualized morphologically and clinically. Specific mechanisms involved in their pathogenesis are proposed.
Controversies and Considerations in the Diagnosis of Primary Cutaneous CD4+ Small/Medium T-Cell Lymphoma
Authors: Lan Thanh T. Ha , Brown Noah A. , Hristov Alexandra C. 
Published: September 2014

Context.—Primary cutaneous CD4+ small/medium T-cell lymphoma is a provisional and controversial entity with a broad differential diagnosis. Despite being an uncommon lymphoma, it is a frequent diagnostic consideration in cutaneous biopsies with a dense lymphoid infiltrate because it shows overlapping features with reactive lymphoid hyperplasia (pseudolymphoma) and a variety of other primary cutaneous and systemic lymphomas. However, proper classification of this process is important for determining patient prognosis and treatment options.
Objective.—To review the clinical, morphologic, immunophenotypic, and genetic features of primary cutaneous CD4+ small/medium T-cell lymphoma and contrast those features with entities in the differential diagnosis.
Data Sources.—Applicable literature will be reviewed with emphasis on current controversies and distinguishing characteristics.
Conclusions.—Although many consider primary cutaneous CD4+ small/medium T-cell lymphoma to be indistinguishable from reactive lymphoid hyperplasia/pseudolymphoma, it can be differentiated from other primary cutaneous and systemic lymphomas. Patients with solitary lesions of primary cutaneous CD4+ small/medium T-cell lymphoma generally have an excellent prognosis. Nevertheless, a subset of patients who have been reported to meet criteria for this lymphoma have followed a more-aggressive course; however, those patients show some differing clinical, morphologic, and immunophenotypic features.
Unveiling the Structure in Mental Disorder Presentations
Authors: Spiller Tobias R. , Duek Or , Helmer Markus , Murray John D. , Fielstein Elliot , Pietrzak Robert H. , von Känel Roland , Harpaz-Rotem Ilan 
Published: August 2024

ImportanceDSM criteria are polythetic, allowing for heterogeneity of symptoms among individuals with the same disorder. In empirical research, most combinations were not found or only rarely found, prompting criticism of this heterogeneity.ObjectiveTo elaborate how symptom-based definitions and assessments contribute to a distinct probability pattern for the occurrence of symptom combinations.Design, Setting, and ParticipantsThis cross-sectional study involved a theoretical argument, simulation, and secondary data analysis of 4 preexisting datasets, each consisting of symptoms from 1 of the following syndromes: posttraumatic stress disorder, depression, schizophrenia, and anxiety. Data were obtained from various sources, including the National Institute of Mental Health Data Archive and Department of Veteran Affairs. A total of 155 474 participants were included (individual studies were 3930 to 63 742 individuals in size). Data were analyzed between July 2021 and January 2024.ExposureFor each participant, the presence or absence of each assessed symptom and their combination was determined. The number of all combinations and their individual frequencies were assessed.Main Outcome and MeasureProbability or frequency of unique symptom combinations and their distribution.ResultsAmong the 155 474 participants, the mean (SD) age was 47.5 (14.8) years; 33 933 (21.8%) self-identified as female and 121 541 (78.2%) as male. Because of the interrelation between symptoms, some symptom combinations were significantly more likely than others. The distribution of the combinations’ probability was heavily skewed with most combinations having a very low probability. Across all 4 empirical samples, the 1% most common combinations were prevalent in a total of 33.1% to 78.6% of the corresponding sample. At the same time, many combinations (ranging from 41.7% to 99.8%) were reported by less than 1% of the sample.Conclusions and RelevanceThis study found that within-disorder symptom heterogeneity followed a specific pattern consisting of few prevalent, prototypical combinations and numerous combinations with a very low probability of occurrence. Future discussions about the revision of diagnostic criteria should take this specific pattern into account by focusing not only on the absolute number of symptom combinations but also on their individual and cumulative probabilities. Findings from clinical populations using common diagnostic criteria may have limited generalizability to the large group of individuals with a low-probability symptom combination.
Balancing ecology and practicality to rank waterbodies for preventative invasive species management
Authors: Roberts Caleb P. , Grant William E. , Horton Matthew L. , LaBrie Lindsey A. P. , Peterson Miranda R. , Rogosch Jane S. , Wang Hsiao‐Hsuan 
Published: August 2024

Abstract

‘Early detection and rapid response’ (EDRR) is the most successful framework for preventative invasive species management, but prioritizing localized EDRR actions with limited resources is challenging. An approach that ranks individual locations, such as waterbodies, for EDRR by combining an invasive species' establishment risk with the practicality of managing it could help set reasonable priorities.

Here, we worked with regional practitioners in Arkansas, USA, and the broader Southeastern USA to co‐produce a workflow for preventative aquatic invasive species management that (1) estimates establishment risk under current and future climates with a species distribution model, (2) scores waterbodies according to difficulty of eradicating an aquatic invasive species if it were introduced and (3) combines establishment risk and eradication difficulty scores to rank waterbodies according to preventative management priority. As our focal species, we used giant salvinia (Salvinia molesta), a floating aquatic fern ranked among the worst weeds in the world due to its negative socio‐ecological impacts and difficulty to eradicate once established.

Current establishment risk is low for much of our study area, but under future climate scenarios (RCP 8.5), areas with >60% giant salvinia establishment risk increased from 546 km2 to 30,219 km2 between 2023 and 2040 in Arkansas.

We found giant salvinia establishment risk and eradication difficulty are independent of each other (r = 0.28), and it follows that, alone, early detection tools such as species distribution models are insufficient for managers to prioritize sites for EDRR.

Practical implication: We envision our approach fitting into a potential EDRR workflow that cascades from broad‐ to local‐scale. To illustrate, (1) horizon scanning and/or climate matching generates lists of high‐risk invasive species; (2) species lists are narrowed according to eradication feasibility scores; (3) for all remaining species, all waterbodies across a geography of interest receive prioritization rankings based on establishment risk and eradication difficulty scores. Given that climate change makes predicting invasive species' distributions a moving target, combining co‐produced eradication difficulty scoring with species distribution modelling will balance rigour with practicality when prioritizing locations for EDRR.
Enhanced Direct Air Carbon Capture on NaX Zeolite by Electric‐Field Enhanced Physical Adsorption and In Situ CO2 Synergistic Effects of Cold Plasma
Authors: Shen Minghai , Kong Fulin , Guo Wei , Zuo Zhongqi , Guo Chan , Tong Lige , Yin Shaowu , Wang Li , Kawi Sibudjing , Chu Paul K. , Ding Yulong 
Published: August 2024

AbstractDirect air carbon capture (DAC) is vital to achieving negative CO2 emissions, with physical adsorption offering a cost‐effective and energy‐efficient solution. an advanced zeolite modification technique is presented using cold plasma, which enhances the CO2 adsorption efficiency of NaX zeolite by 11.5% after just 60 min. This method utilizes the electric field to reorganize cation distribution and pore structure and significantly improve the adsorption capacity, efficiency, and selectivity. Compared to traditional methods, this technique is simpler and more effective, as demonstrated through first‐principles calculations, thermodynamics, kinetics, and adsorption equilibrium studies.
Compatibilizer Effects of Strategically Designed Donor–Acceptor Block Copolymers to Enhance the Performance, Stability, and Mechanical Durability of Inverted Organic Solar Cells
Authors: Tseng Yu‐Cheng , Fan Qunping , Tsai Chu‐Yen , Chang Jia‐Fu , Yu Ming‐Hsuan , Tseng Hsu‐Zi , Yip Hin‐Lap , Lin Francis R. , Jen Alex K.‐Y. , Chueh Chu‐Chen 
Published: August 2024

AbstractA strategically designed donor–acceptor (D‐A) block copolymer (PM6‐b‐PYIT) is introduced, as a compatibilizer to enhance the performance and stability of inverted organic solar cells (OSCs) consisting of a bulk heterojunction (BHJ) of PM6 and L8‐BO. The PM6‐b‐PYIT compatibilizer not only significantly boosts the power conversion efficiency from 16.32% to 18.02%, but also further modulates the molecular arrangement and improves the compatibility between donor and acceptor materials. This stems from the structural similarity between the copolymer and the host materials, which facilitates ordered molecular stacking and superior charge‐transporting properties, thereby improving the dielectric constant and built‐in voltage and mitigating excessive charge recombination. More importantly, the role of the compatibilizer in stabilizing the BHJ morphology under long‐term aging conditions is highlighted, ascribed to the improved miscibility between different components in the BHJ composite. This in turn renders the photoactive layer more mechanically durable, making it suitable for stretchable applications.
Mixed‐Ligand Chiral Quasi‐2D Perovskites for Standard and Deep Blue CP‐LEDs
Authors: Yan Xinyao , Cao Rui , Zhang Ruxi , Gao Haotian , Xiao Yin 
Published: August 2024

AbstractThis study presents the development of mixed‐ligand quasi‐2D perovskites ((2‐PEA)x(R/S‐2‐BA)2MA1Cs1Pb3Br10+x, x = 0.8, 1.6, 2.4) for circularly polarized light‐emitting diodes (CP‐LEDs). By utilizing the strong confinement effect of 2‐phenylethylammonium (2‐PEA) and the chirality of R/S‐2‐butylammonium (R/S‐2‐BA), room‐temperature circularly polarized luminescence (CPL) across the green to deep blue spectrum is achieved. The green CPL attributes to the transfer of spin‐polarized excitons while the blue CPL originates from direct excitation of luminescent phases with intrinsic chirality. Finally, green and blue CP‐LEDs under ambient are realized. Notably, standard and deep blue CP‐EL is achieved based on 0.8PEA/(R/S‐2‐BA)‐ and 1.2PEA/(R/S‐2‐BA)‐films with impressive electroluminescent asymmetry factors (0.122 and 0.175), highlighting the potential of chiral perovskites in CP‐LEDs and opening avenues for the development of room‐temperature full‐color CP‐EL display.
Infrared‐Scattering by Programmable Peptide‐Melanin Microparticles
Authors: Lee Di Sheng , Yuan Weize , Voigt Christopher A. 
Published: August 2024

AbstractNatural melanin polymers, biosynthesized from free tyrosine, assemble into microstructures that interact with light, producing a wide range of photonic properties. These structures are formed by mechanisms that are difficult to control to create new optical materials. Here, simple pentapeptides YXXXY is presented, where the XXX controls self‐assembly into microstructures, after which the Y's are polymerized by tyrosinase into melanin. It sought to computationally predict peptides that will assemble into two‐layer films that exhibit structural color and infrared (IR) scattering. Three peptides (YPIIY, YLPIY, YVPAY) are synthesized and found to form a two‐layer film exhibiting structural color. YLPIY‐melanin also forms a stable coating with scattering peaks in the near‐ and mid‐IR. This coating reduces the signature of a hotplate (37 °C) as seen by a thermal camera. This synthetic peptidic material is the first to exhibit IR‐scattering, opening the possibility of incorporating this function into bio‐compatible fibers, textiles, and plastics.