bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antithrombin up-regulates AMP-activated protein kinase signalling during myocardial ischaemia/reperfusion injury
Authors: Ma Yina , Wang Jinli , Gao Junjie , Yang Hui , Wang Yanqing , Manithody Chandrashekhara , Li Ji , Rezaie Alireza R. 
Published: September 2014

SummaryAntithrombin (AT) is a protein of the serpin superfamily involved in regulation of the proteolytic activity of the serine proteases of the coagulation system. AT is known to exhibit anti-inflammatory and cardioprotective properties when it binds to heparan sulfate proteo - glycans (HSPGs) on vascular cells. AMP-activated protein kinase (AMPK) plays an important cardioprotective role during myocardial ischaemia and reperfusion (I/R). To determine whether the cardioprotective signaling function of AT is mediated through the AMPK pathway, we evaluated the cardioprotective activities of wild-type AT and its two derivatives, one having high affinity and the other no affinity for heparin, in an acute I/R injury model in C57BL/6J mice in which the left anterior descending coronary artery was occluded. The serpin derivatives were given 5 minutes before reperfusion. The results showed that AT-WT can activate AMPK in both in vivo and ex vivo conditions. Blocking AMPK activity abolished the cardioprotective function of AT against I/R injury. The AT derivative having high affinity for heparin was more effective in activating AMPK and in limiting infraction, but the derivative lacking affinity for heparin was inactive in eliciting AMPK-dependent cardioprotective activity. Activation of AMPK by AT inhibited the inflammatory c-Jun N-terminal protein kinase (JNK) pathway during I/R. Further studies revealed that the AMPK activity induced by AT also modulates cardiac substrate metabolism by increasing glucose oxidation but inhibiting fatty acid oxidation during I/R. These results suggest that AT binds to HSPGs on heart tissues to invoke a cardioprotective function by triggering cardiac AMPK activation, thereby attenuating JNK inflammatory signalling pathways and modulating substrate metabolism during I/R.
Loss of cysteine 584 impairs the storage and release, but not the synthesis of von Willebrand factor
Authors: Daidone Viviana , Barbon Giovanni , Pontara Elena , Cattini Grazia , Gallinaro Lisa , Zampese Enrico , Pizzo Paola , Casonato Alessandra 
Published: September 2014

SummaryCysteines play a key part in von Willebrand factor (VWF) dimerisation and polymerisation, and their loss may severely affect VWF structure and function. We report on three patients with type 3 von Willebrand disease carrying the new c.1751G>T missense mutation that induces the substitution of cysteine 584 by phenylalanine (C584F), and the deletion of seven nucleotides in exon 7 (c.729_735del), producing a premature stop codon at position 454 (E244Lfs*211). VWF was almost undetectable in the patients’ plasma and platelets, while a single, poorly represented, oligomer emerged on plasma VWF multimer analysis. No post-DDAVP increase in VWF and factor VIII was observed. Expressing human recombinant C584F-VWF in HEK293T cells showed that C584F-VWF was synthesised and multimerised but not secreted – apart from the first oligomer, which was slightly represented in the conditioned medium, with a pattern similar to the patients’ plasma VWF. The in vitro expression of the E244Lfs*211–VWF revealed a defective synthesis of the mutated VWF, with a behavior typical of loss of function mutations. Cellular trafficking, investigated in HEK293 cells, indicated a normal C584F-VWF content in the endoplasmic reticulum and Golgi apparatus, confirming the synthesis and multimerisation of C584F-VWF. No pseudo-Weibel Palade bodies were demonstrable, however, suggesting that C584F mutation impairs the storage of C584F-VWF. These findings point to cysteine 584 having a role in the release of VWF and its targeting to pseudo-Weibel Palade bodies in vitro, as well as in its storage and release by endothelial cells in vivo.
Cytoprotective-selective activated protein C therapy for ischaemic stroke
Authors: Zlokovic Berislav , Griffin John , Mosnier Laurent 
Published: September 2014

SummaryDespite years of research and efforts to translate stroke research to clinical therapy, ischaemic stroke remains a major cause of death, disability, and diminished quality of life. Primary and secondary preventive measures combined with improved quality of care have made significant progress. However, no novel drug for ischaemic stroke therapy has been approved in the past decade. Numerous studies have shown beneficial effects of activated protein C (APC) in rodent stroke models. In addition to its natural anticoagulant functions, APC conveys multiple direct cytoprotective effects on many different cell types that involve multiple receptors including protease activated receptor (PAR) 1, PAR3, and the endothelial protein C receptor (EPCR). Application of molecular engineered APC variants with altered selectivity profiles to rodent stroke models demonstrated that the beneficial effects of APC primarily require its cytoprotective activities but not its anticoagulant activities. Extensive basic, preclinical, and clinical research provided a compelling rationale based on strong evidence for translation of APC therapy that has led to the clinical development of the cytoprotectiveselective APC variant, 3K3A-APC, for ischaemic stroke. Recent identification of non-canonical PAR1 and PAR3 activation by APC that give rise to novel tethered-ligands capable of inducing biased cytoprotective signalling as opposed to the canonical signalling provides a mechanistic explanation for how APC-mediated PAR activation can selectively induce cytoprotective signalling pathways. Collectively, these paradigm-shifting discoveries provide detailed insights into the receptor targets and the molecular mechanisms for neuroprotection by cytoprotective-selective 3K3A-APC, which is currently a biologic drug in clinical trials for ischaemic stroke.
Records of chimaeroid fishes (Holocephali: Chimaeriformes) from the Pacific coast of Costa Rica, with the description of a new species of Chimera (Chimaeridae) from the eastern Pacific Ocean
Authors: ANGULO ARTURO , LÓPEZ MYRNA I. , BUSSING WILLIAM A. , MURASE ATSUNOBU 
Published: September 2014

A new species of Chimaera Linnaeus 1758 is described from three specimens collected from off the Pacific coasts of Costa Rica and Peru. Chimaera orientalis n. sp., the first species of the genus described from the eastern Pacific Ocean, is distinguished from its other congeners by a combination of coloration and morphology. Additionally, new records of occurrence for another four species of chimaeroid fishes (Harriotta raleighana (Goode & Bean 1895), Rhinochimaera africana Compagno, Stehmann & Ebert 1990, Hydrolagus colliei Lay & Bennett 1839, and H. macrophthalmus de Buen 1959) previously unknown for the continental shelf of the Pacific coast of Costa Rica and Central America are reported. A key to the eastern Pacific species of the order Chimaeriformes is also presented.
Extension of the TAMSAT Satellite-Based Rainfall Monitoring over Africa and from 1983 to Present
Authors: Tarnavsky Elena , Grimes David , Maidment Ross , Black Emily , Allan Richard P. , Stringer Marc , Chadwick Robin , Kayitakire Francois 
Published: September 2014

AbstractTropical Applications of Meteorology Using Satellite Data and Ground-Based Observations (TAMSAT) rainfall monitoring products have been extended to provide spatially contiguous rainfall estimates across Africa. This has been achieved through a new, climatology-based calibration, which varies in both space and time. As a result, cumulative estimates of rainfall are now issued at the end of each 10-day period (dekad) at 4-km spatial resolution with pan-African coverage. The utility of the products for decision making is improved by the routine provision of validation reports, for which the 10-day (dekadal) TAMSAT rainfall estimates are compared with independent gauge observations. This paper describes the methodology by which the TAMSAT method has been applied to generate the pan-African rainfall monitoring products. It is demonstrated through comparison with gauge measurements that the method provides skillful estimates, although with a systematic dry bias. This study illustrates TAMSAT’s value as a complementary method of estimating rainfall through examples of successful operational application.
Occurrence of Increased Arterial Stiffness in a Cohort of Adult Patients With Type 1 Diabetes Mellitus When Compared to Normoglycemic Controls
Authors: George Belinda , Bantwal Ganapathi , Ayyar Vageesh , Mathew Vivek 
Published: September 2014

Objectives: The prevalence of cardiovascular risk factors among patients with type 1 diabetes is underestimated. This study was conducted to assess the cardiovascular risk by comparing arterial stiffness in type 1 diabetes with that of controls. Research design and methods: Thirty-one type 1 diabetes patients and 31 age- and gender-matched controls were included. The brachial-ankle pulse wave velocity (ba-PWV) was measured using Periscope, a noninvasive device. Results: The ba-PWV was found to be significantly elevated among the patients with diabetes (1177.09 ± 180.1 cm/s) when compared with the controls (1057.99 ± 95.8 cm/s) ( P = .003). The ba-PWV showed positive correlation with age ( r = .43, P = .014), heart rate ( r = .49, P = .005), blood pressure ( r = .65, P < .001), and LDL ( r = .46, P = .008) among patients with diabetes. Linear regression analysis revealed that diabetes, age, systolic blood pressure (SBP), and BMI were independently associated with ba-PWV ( R2 = .589). On subdividing the diabetes cohort into quintiles for LDL and SBP, a substantial increase in ba-PWV was seen at LDL > 102 mg/dl (1187.8 cm/s to 1364.9 cm/s, P = .09) and SBP > 130 mmHg (1176.1 cm/s to 1369.4 cm/s, P = .03). Conclusions: Arterial stiffness as assessed by ba-PWV is significantly increased among patients with type 1 diabetes compared to healthy controls, independent of traditional cardiovascular risk factors. Early screening for arterial stiffness using a cheap and effective method among patients with SBP > 130 mmHg and LDL > 102 mg/dl may help identify those with heightened cardiovascular risk.
Splenic TFH expansion participates in B-cell differentiation and antiplatelet-antibody production during immune thrombocytopenia
Authors: Audia Sylvain , Rossato Marzia , Santegoets Kim , Spijkers Sanne , Wichers Catharina , Bekker Cornelis , Bloem Andries , Boon Louis , Flinsenberg Thijs , Compeer Ewoud , van den Broek Theo , Facy Olivier , Ortega-Deballon Pablo , Berthier Sabine , Leguy-Seguin Vanessa , Martin Laurent , Ciudad Marion , Samson Maxime , Trad Malika , Lorcerie Bernard , Janikashvili Nona , Saas Philippe , Bonnotte Bernard , Radstake Timothy R. D. J. 
Published: September 2014

Key Points
Human splenic TFH expansion during ITP participates in B-cell differentiation and antiplatelet-antibody production. IL-21 and CD40 are key TFH molecules that could be promising targets in the treatment of ITP.
Antibody Fc engineering improves frequency and promotes kinetic boosting of serial killing mediated by NK cells
Authors: Romain Gabrielle , Senyukov Vladimir , Rey-Villamizar Nicolas , Merouane Amine , Kelton William , Liadi Ivan , Mahendra Ankit , Charab Wissam , Georgiou George , Roysam Badrinath , Lee Dean A. , Varadarajan Navin 
Published: September 2014

Key Points
Fc-engineered mAb promotes NK cell ADCC via better activation, serial killing, and kinetic boosting at higher target cell densities. Enhanced target killing also increased frequency of NK cell apoptosis, but this effect is donor-dependent.
In vitro evidence of a tissue factor-independent mode of action of recombinant factor VIIa in hemophilia
Authors: Augustsson Cecilia , Persson Egon 
Published: September 2014

Key PointsThe negative impact on thrombin generation of zymogen FVII competing with rFVIIa for TF is counteracted by FVII (auto)activation. Correction of hemophilia A occurs in a rFVIIa concentration range where detectable effects of FVII competition are minimal or absent.
Clinical heterogeneity and predictors of outcome in primary autoimmune hemolytic anemia: a GIMEMA study of 308 patients
Authors: Barcellini Wilma , Fattizzo Bruno , Zaninoni Anna , Radice Tommaso , Nichele Ilaria , Di Bona Eros , Lunghi Monia , Tassinari Cristina , Alfinito Fiorella , Ferrari Antonella , Leporace Anna Paola , Niscola Pasquale , Carpenedo Monica , Boschetti Carla , Revelli Nicoletta , Villa Maria Antonietta , Consonni Dario , Scaramucci Laura , De Fabritiis Paolo , Tagariello Giuseppe , Gaidano Gianluca , Rodeghiero Francesco , Cortelezzi Agostino , Zanella Alberto 
Published: September 2014

Key Points
Mixed, atypical, and warm immunoglobulin G plus C AIHA (∼30% of cases) more frequently have a severe onset (Hb ≤6 g/dL) and require multiple therapy lines. Infections, particularly after splenectomy, acute renal failure, Evans syndrome, and multitreatment, were predictors of fatal outcome.
Efficient solar photocatalyst based on cobalt oxide/iron oxide composite nanofibers for the detoxification of organic pollutants
Authors: Asif Safi Asim Bin , Khan Sher Bahadar , Asiri Abdullah M 
Published: September 2014

Abstract
A Co3O4/Fe2O3 composite nanofiber-based solar photocatalyst has been prepared, and its catalytic performance was evaluated by degrading acridine orange (AO) and brilliant cresyl blue (BCB) beneath solar light. The morphological and physiochemical structure of the synthesized solar photocatalyst was characterized by X-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR). FESEM indicates that the Co3O4/Fe2O3 composite has fiber-like nanostructures with an average diameter of approximately 20 nm. These nanofibers are made of aggregated nanoparticles having approximately 8.0 nm of average diameter. The optical properties were examined by UV-visible spectrophotometry, and the band gap of the solar photocatalyst was found to be 2.12 eV. The as-grown solar photocatalyst exhibited high catalytic degradation in a short time by applying to degrade AO and BCB. The pH had an effect on the catalytic performance of the as-grown solar photocatalyst, and it was found that the synthesized solar photocatalyst is more efficient at high pH. The kinetics study of both AO and BCB degradation indicates that the as-grown nanocatalyst would be a talented and efficient solar photocatalyst for the removal of hazardous and toxic organic materials.
Novel somatic single nucleotide variants within the RNA binding protein hnRNP A1 in multiple sclerosis patients
Authors: Lee Sangmin , Levin Michael 
Published: September 2014

Some somatic single nucleotide variants (SNVs) are thought to be pathogenic, leading to neurological disease. We hypothesized that heterogeneous nuclear ribonuclear protein A1 (hnRNP A1), an autoantigen associated with multiple sclerosis (MS) would contain SNVs. MS patients develop antibodies to hnRNP A1293-304, an epitope within the M9 domain (AA268-305) of hnRNP A1. M9 is hnRNP A1’s nucleocytoplasmic transport domain, which binds transportin-1 (TPNO-1) and allows for hnRNP A1’s transport into and out of the nucleus. Genomic DNA sequencing of M9 revealed nine novel SNVs that resulted in an amino acid substitution in MS patients that were not present in controls. SNVs occurred within the TPNO-1 binding domain (hnRNP A1268-289) and the MS IgG epitope (hnRNP A1293-304), within M9. In contrast to the nuclear localization of wild type (WT) hnRNP A1, mutant hnRNP A1 mis-localized to the cytoplasm, co-localized with stress granules and caused cellular apoptosis. Whilst WT hnRNP A1 bound TPNO-1, mutant hnRNP A1 showed reduced TPNO-1 binding. These data suggest SNVs in hnRNP A1 might contribute to pathogenesis of MS.
A Liouville comparison principle for solutions of
quasilinear singular parabolic inequalities
Authors: Kurta Vasilii V. 
Published: September 2014

Abstract
We obtain a Liouville comparison principle for entire weak
solutions (u,v) of quasilinear singular parabolic second-order
partial differential inequalities of the form

u
t

-
A

(
u
)

-

|
u
|

q
-
1

u
≥

v
t

-
A

(
v
)

-

|
v
|

q
-
1

v

${ u_t -
A(u)-|u|^{q-1}u \ge v_t - A (v)-|v|^{q-1}v }$

on the set

𝕊
τ

=

(
τ
,
+
∞
)

×

ℝ

n

${{\mathbb {S}}_{\tau }= (\tau , +\infty )\times {\mathbb {R}}^n}$

, where q > 0, n ≥ 1, τ is a real number or

τ
=
-
∞

${\tau =-\infty }$

, and the
differential operator A satisfies the α-monotonicity
condition. Model examples of the operator A in our study are the
well-known p-Laplacian operator defined by the relation

Δ
p

(
w
)

=

div
x

(
|

∇
x

w

|

p
-
2

∇
x

w
)

${\Delta _p
(w)=\operatorname{div}_x(|\nabla _x w|^{p-2}\nabla _x w) }$

and its
well-known modification defined by

Δ
˜

p

(
w
)

=

∑

i
=
1

n

∂

∂

x
i

(
|

∂
w

∂

x
i

|

p
-
2

∂
w

∂

x
i

)

${ \widetilde{\Delta }_p (w) =
\sum _{i=1}^n\frac{\partial }{\partial x_i}( |\frac{\partial w}{\partial x_i}|^{p-2}\frac{\partial w}{\partial x_i})}$

.
Mechanical stability of superhydrophobic epoxy/silica coating for better water resistance of wood
Authors: Liu Feng , Gao Zhengxin , Zang Deli , Wang Chengyu , Li Jian 
Published: September 2014

Abstract
A three-step procedure has been developed for superhydrophobic coating on wood based on epoxy/silica materials in combination with hydrophobization. First, the epoxy resin is adhered to wood by immersing the samples into an epoxy resin acetone solution, then amino-functionalized silica particles are anchored by the epoxide groups, and finally, the created surface is modified by octadecyltrichlorosilane (OTS). The superhydrophobic surface not only is water repellent, as shown by the contact angle (CA) tests, but also decreases essentially the wood’s water absorption as determined by a 120-day water immersion test. The good mechanical stability of the coating was confirmed by a sand collision method.
Preparation of anionic clathrate-II K24-x
Ge136 by filling of Ge(cF136)
Authors: Böhme Bodo , Reibold Marianne , Auffermann Gudrun , Lichte Hannes , Baitinger Michael , Grin Yuri 
Published: September 2014

Abstract
Metastable Ge(cF136) with empty clathrate-II crystal structure was successfully used for the preparation of otherwise hardly accessible germanium clathrate phases. On reaction of Ge(cF136) with potassium vapour at 280 °C for 6 days, the new clathrate-II phase K24Ge136 was formed, in which the cages of the germanium framework are completely filled by potassium atoms. The crystal structure of K
x
Ge136 is discussed for different potassium contents (x=0, 8.6, 24).
Whole-Genome Analysis of Multienvironment or Multitrait QTL in MAGIC
Authors: Verbyla Arūnas P , Cavanagh Colin R , Verbyla Klara L 
Published: September 2014

Abstract
Multiparent Advanced Generation Inter-Cross (MAGIC) populations are now being utilized to more accurately identify the underlying genetic basis of quantitative traits through quantitative trait loci (QTL) analyses and subsequent gene discovery. The expanded genetic diversity present in such populations and the amplified number of recombination events mean that QTL can be identified at a higher resolution. Most QTL analyses are conducted separately for each trait within a single environment. Separate analysis does not take advantage of the underlying correlation structure found in multienvironment or multitrait data. By using this information in a joint analysis—be it multienvironment or multitrait — it is possible to gain a greater understanding of genotype- or QTL-by-environment interactions or of pleiotropic effects across traits. Furthermore, this can result in improvements in accuracy for a range of traits or in a specific target environment and can influence selection decisions. Data derived from MAGIC populations allow for founder probabilities of all founder alleles to be calculated for each individual within the population. This presents an additional layer of complexity and information that can be utilized to identify QTL. A whole-genome approach is proposed for multienvironment and multitrait QTL analysis in MAGIC. The whole-genome approach simultaneously incorporates all founder probabilities at each marker for all individuals in the analysis, rather than using a genome scan. A dimension reduction technique is implemented, which allows for high-dimensional genetic data. For each QTL identified, sizes of effects for each founder allele, the percentage of genetic variance explained, and a score to reflect the strength of the QTL are found. The approach was demonstrated to perform well in a small simulation study and for two experiments, using a wheat MAGIC population.
Usefulness of Multiparental Populations of Maize (Zea mays L.) for Genome-Based Prediction
Authors: Lehermeier Christina , Krämer Nicole , Bauer Eva , Bauland Cyril , Camisan Christian , Campo Laura , Flament Pascal , Melchinger Albrecht E , Menz Monica , Meyer Nina , Moreau Laurence , Moreno-González Jesús , Ouzunova Milena , Pausch Hubert , Ranc Nicolas , Schipprack Wolfgang , Schönleben Manfred , Walter Hildrun , Charcosset Alain , Schön Chris-Carolin 
Published: September 2014

Abstract
The efficiency of marker-assisted prediction of phenotypes has been studied intensively for different types of plant breeding populations. However, one remaining question is how to incorporate and counterbalance information from biparental and multiparental populations into model training for genome-wide prediction. To address this question, we evaluated testcross performance of 1652 doubled-haploid maize (Zea mays L.) lines that were genotyped with 56,110 single nucleotide polymorphism markers and phenotyped for five agronomic traits in four to six European environments. The lines are arranged in two diverse half-sib panels representing two major European heterotic germplasm pools. The data set contains 10 related biparental dent families and 11 related biparental flint families generated from crosses of maize lines important for European maize breeding. With this new data set we analyzed genome-based best linear unbiased prediction in different validation schemes and compositions of estimation and test sets. Further, we theoretically and empirically investigated marker linkage phases across multiparental populations. In general, predictive abilities similar to or higher than those within biparental families could be achieved by combining several half-sib families in the estimation set. For the majority of families, 375 half-sib lines in the estimation set were sufficient to reach the same predictive performance of biomass yield as an estimation set of 50 full-sib lines. In contrast, prediction across heterotic pools was not possible for most cases. Our findings are important for experimental design in genome-based prediction as they provide guidelines for the genetic structure and required sample size of data sets used for model training.