bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unraveling the cytotoxic potential of Temozolomide loaded into PLGA nanoparticles
Authors: Jain Darshana S , Athawale Rajani B , Bajaj Amrita N , Shrikhande Shruti S , Goel Peeyush N , Nikam Yuvraj , Gude Rajiv P 
Published: January 2014

Abstract

Background
Nanotechnology has received great attention since a decade for the treatment of different varieties of cancer. However, there is a limited data available on the cytotoxic potential of Temozolomide (TMZ) formulations. In the current research work, an attempt has been made to understand the anti-metastatic effect of the drug after loading into PLGA nanoparticles against C6 glioma cells.
Nanoparticles were prepared using solvent diffusion method and were characterized for size and morphology. Diffusion of the drug from the nanoparticles was studied by dialysis method. The designed nanoparticles were also assessed for cellular uptake using confocal microscopy and flow cytometry.

Results
PLGA nanoparticles caused a sustained release of the drug and showed a higher cellular uptake. The drug formulations also affected the cellular proliferation and motility.

Conclusion
PLGA coated nanoparticles prolong the activity of the loaded drug while retaining the anti-metastatic activity.
Characterisation of interleukin‐10 expression on different vascular structures in allergic nasal mucosa
Authors: Muller Barbara , van Egmond Danielle , de Groot Esther JJ , Fokkens Wytske J , van Drunen Cornelis M 
Published: January 2014

AbstractBackgroundInterleukin‐10 (IL‐10) is a negative regulator of immune responses and was previously shown to be expressed by human nasal endothelial cells, while the adhesion molecule MECA‐79 plays a role in trans‐endothelial migration of immune competent cells. In this study we investigate the relationship between endothelial IL‐10 and MECA‐79 expression to address the question whether immune competent cells could be affected at the mucosal entry site.MethodsNasal turbinate biopsies were taken from house dust mite allergic patients, before and after nasal allergen provocation. Subsequent slides of biopsies were stained for IL10, MECA‐79, CD34, and IL10‐Receptor. Capillaries, arteries/veins, and sinusoids were evaluated separately.Results90% of sinusoids are IL‐10 positive and all sinusoids are negative for MECA‐79, while 4.8% of capillaries are positive for IL‐10, and 2.2% are positive for MECA‐79. Although about 47% of arteries/veins are positive for IL‐10 and 57.1% are positive for MECA‐79, only about 20% are positive for both markers. Furthermore, we showed that the myo‐fibroblasts surrounding all sinusoids stain positive for IL10R.ConclusionsIL10 expression on vascular structures is not related to MECA expression for sinusoids and capillaries and only partly related on arteries/veins, however sinusoidal endothelial IL10 expression is always seen in combination with IL‐10R expression of sinusoidal myo‐fibroblasts.
Ontogeny of the digestive system of the Octopus bimaculatus paralarvae (Verril, 1883)
Authors: López-Peraza Diana Judith , Hernández-Rodríguez Mónica , Barón-Sevilla Benjamín 
Published: January 2014

AbstractThe high mortalities registered in the larval stage during octopus culturing are mainly due to nutritional deficiencies of the food provided. To understand the cause of this problem, we studied the ontogenetic development of the digestive system ofOctopus bimaculatusparalarvae. An egg batch was obtained from a gravid female collected in the Bay of Los Angeles, Baja California, Mexico, and it was incubated in the laboratory during the summer of 2011. We observed that the formation of the digestive system began at 33 days post-laying (DPL). The newly hatched paralarvae had already formed the organs involved in food ingestion and digestion, although it was not possible to know accurately their degree of maturity. The present research constitutes the first description at the histological level of the ontogenic development of the digestive system of theO. bimaculatusparalarvae. This serves as a basis for future studies of the digestive physiology of this species.
Characterization of T cell phenotype and function in a double transgenic (collagen-specific TCR/HLA-DR1) humanized model of arthritis
Authors: Tang Bo , Kim Seunghyun , Hammond Sarah , Cullins David L , Brand David D , Rosloniec Edward F , Stuart John M , Postlethwaite Arnold E , Kang Andrew H , Myers Linda K 
Published: January 2014

Abstract
Introduction
T cells orchestrate joint inflammation in rheumatoid arthritis (RA), yet they are difficult to study due to the small numbers of antigen-specific cells. The goal of this study was to characterize a new humanized model of autoimmune arthritis and to describe the phenotypic and functional changes that occur in autoimmune T cells following the induction of pathological events.

Methods
We developed a double transgenic mouse containing both the HLA-DR1 transgene and an HLA-DR1-restricted collagen-specific TCR in order to obtain large numbers of antigen-specific T cells that can be used for immunologic studies.

Results
In vitro, CII-specific T cells from this mouse proliferated vigorously in response to the CII immunodominant peptide A2 and the cells altered their phenotype to become predominately CD62Llow and CD44high “activated” T cells. The response was accompanied by the production of Th1, Th2, and Th17-type cytokines. Following immunization with bovine CII/CFA, these mice develop an accelerated arthritis compared to single transgenic HLA-DR1 mice. On the other hand, when the mice were treated orally with the analog peptide A12, (a suppressive analog of collagen we have previously described), arthritis was significantly suppressed, despite the fact that >90% of the CD4+ T cells express the TCR Tg. In GALT tissues taken from the A12-treated mice, IL-2, IFN-γ, and IL-17 production to the autoimmune collagen determinant dropped while high levels of IL-10 and IL-4 were produced.

Conclusions
We have developed a humanized model of autoimmune arthritis that will be useful for the study of T cell directed therapies as well as T cell mediated mechanisms of autoimmune diseases.
The effect of continuous versus intermittent renal replacement therapy on the outcome of critically ill patients with acute renal failure (CONVINT): a prospective randomized controlled trial
Authors: Schefold Joerg C , Haehling Stephan von , Pschowski Rene , Bender Thorsten Onno , Berkmann Cathrin , Briegel Sophie , Hasper Dietrich , Jörres Achim 
Published: January 2014

Abstract
Introduction
Acute renal failure (ARF) requiring renal replacement therapy (RRT) occurs frequently in ICU patients and significantly affects mortality rates. Previously, few large clinical trials investigated the impact of RRT modalities on patient outcomes. Here we investigated the effect of two major RRT strategies (intermittent hemodialysis (IHD) and continuous veno-venous hemofiltration (CVVH)) on mortality and renal-related outcome measures.

Methods
This single-center prospective randomized controlled trial (“CONVINT”) included 252 critically ill patients (159 male; mean age, 61.5 ± 13.9 years; Acute Physiology and Chronic Health Evaluation (APACHE) II score, 28.6 ± 8.8) with dialysis-dependent ARF treated in the ICUs of a tertiary care academic center. Patients were randomized to receive either daily IHD or CVVH. The primary outcome measure was survival at 14 days after the end of RRT. Secondary outcome measures included 30-day-, intensive care unit-, and intrahospital mortality, as well as course of disease severity/biomarkers and need for organ-support therapy.

Results
At baseline, no differences in disease severity, distributions of age and gender, or suspected reasons for acute renal failure were observed. Survival rates at 14 days after RRT were 39.5% (IHD) versus 43.9% (CVVH) (odds ratio (OR), 0.84; 95% confidence interval (CI), 0.49 to 1.41; P = 0.50). 14-day-, 30-day, and all-cause intrahospital mortality rates were not different between the two groups (all P > 0.5). No differences were observed in days on RRT, vasopressor days, days on ventilator, or ICU-/intrahospital length of stay.

Conclusions
In a monocentric RCT, we observed no statistically significant differences between the investigated treatment modalities regarding mortality, renal-related outcome measures, or survival at 14 days after RRT. Our findings add to mounting data demonstrating that intermittent and continuous RRTs may be considered equivalent approaches for critically ill patients with dialysis-dependent acute renal failure.

Trial registration
NCT01228123, clinicaltrials.gov
Human umbilical cord tissue-derived mesenchymal stromal cells attenuate remodeling after myocardial infarction by proangiogenic, antiapoptotic, and endogenous cell-activation mechanisms
Authors: Nascimento Diana Santos , Mosqueira Diogo , Sousa Luís Moura , Teixeira Mariana , Filipe Mariana , Resende Tatiana Pinho , Araújo Ana Francisca , Valente Mariana , Almeida Joana , Martins José Paulo , Santos Jorge Miguel , Bárcia Rita Nogueira , Cruz Pedro , Cruz Helder , Pinto-do-Ó Perpétua 
Published: January 2014

Abstract
Introduction
Among the plethora of cells under investigation to restore a functional myocardium, mesenchymal stromal cells (MSCs) have been granted considerable interest. However, whereas the beneficial effects of bone marrow MSCs (BM-MSCs) in the context of the diseased heart are widely reported, data are still scarce on MSCs from the umbilical cord matrix (UCM-MSCs). Herein we report on the effect of UCM-MSC transplantation to the infarcted murine heart, seconded by the dissection of the molecular mechanisms at play.

Methods
Human umbilical cord tissue-derived MSCs (UCX®), obtained by using a proprietary technology developed by ECBio, were delivered via intramyocardial injection to C57BL/6 females subjected to permanent ligation of the left descending coronary artery. Moreover, medium produced by cultured UCX® preconditioned under normoxia (CM) or hypoxia (CMH) was collected for subsequent in vitro assays.

Results
Evaluation of the effects upon intramyocardial transplantation shows that UCX® preserved cardiac function and attenuated cardiac remodeling subsequent to myocardial infarction (MI). UCX® further led to increased capillary density and decreased apoptosis in the injured tissue. In vitro, UCX®-conditioned medium displayed (a) proangiogenic activity by promoting the formation of capillary-like structures by human umbilical vein endothelial cells (HUVECs), and (b) antiapoptotic activity in HL-1 cardiomyocytes subjected to hypoxia. Moreover, in adult murine cardiac Sca-1+ progenitor cells (CPCs), conditioned medium enhanced mitogenic activity while activating a gene program characteristic of cardiomyogenic differentiation.

Conclusions
UCX® preserve cardiac function after intramyocardial transplantation in a MI murine model. The cardioprotective effects of UCX® were attributed to paracrine mechanisms that appear to enhance angiogenesis, limit the extent of the apoptosis, augment proliferation, and activate a pool of resident CPCs. Overall, these results suggest that UCX® should be considered an alternative cell source when designing new therapeutic approaches to treat MI.
Cable arrangement to reduce electromagnetic coupling effects in spectral-induced polarization studies
Authors: Schmutz Myriam , Ghorbani Ahmad , Vaudelet Pierre , Blondel Amélie 
Published: January 2014

Spectral-induced polarization (SIP) is widely used for environmental and engineering geophysical prospecting and hydrogeophysics, but one major limitation concerns the electromagnetic (EM) coupling effect. The phase angles related to EM coupling may increase even at frequencies as low as 1 Hz, depending on the ground resistivity, the array type, and the geometry. Most efforts to understand and quantify the EM coupling problem (e.g., theory and computer codes) have been developed for dipole-dipole arrays. However, we used a Schlumberger array to acquire SIP data. We found that with this array, the use of an appropriate cable arrangement during data acquisition can reduce EM coupling effects in the same proportion as for the use of a dipole-dipole array, which is the pure response of the studied earth. To measure the influence of the cable layout, four cable configurations with the same electrode spacing were compared for modeling and experimental data. We discovered that the classical DC inline array was the worst one. As soon as the cables were arranged in another shape (triangle or rectangle), the coupling effect decreased significantly. The best configuration we checked was the rectangular one with an acquisition unit located at a lateral offset of 100 m from the electrode line, even if there was still some difference between the modeled and measured data.
Glabolizatsiya economics and management network in the interaction of national economic systems
Authors: Галайда  , Galayda V. 
Published: January 2014

The article deals with the processes of globalization of the economy and provides the mechanisms of its regulation. It reveals the important components of the scientific school of interaction between government, business and society professor Ukolov VF and Professor IK Bystriakov and state the basic foundation using
the results of its work in practice.
Network communication in knowledge management directions and perspectives of use
Authors: Смирнова  , Smirnova Valyentina 
Published: January 2014

The new emerging communicational possibilities of global network in knowledge management are
considered. The communicative competence is defined as a dynamic capability of organization that
provides its efficiency and competitiveness. The external structure of intellectual capital of modern company
is examined from a new perspective, whose important part is becoming Internet users. Their active role in
the ideas generation, knowledge sharing, innovation development, and training is shown. The methods
and forms of networking such as communities, forums, blogs, crowdsourcing, and social networking which
foreign and Russian companies implement in dealing with users in knowledge management are studied. The
trends and prospects of company network communication development for increasing its intellectual capital
and achieving sustainable com-petitive advantage are defined.
Electronic Textbook: Pros and Contras
Authors: Виноградова  , Vinogradova Natalya 
Published: January 2014

The paper deals with argumentation on a
challenging problem of introducing electronic edition of
textbook for the primary school. Different opinions are
presented. Teachers, educational supervisors, pediatric
psychologists and schoolchildren’s parents articulate their
concerns on reasonability of replacing paper textbook with
its electronic version at the primary school, articulate their
different opinions on functions of electronic training
literature in the educational process, on requirements to
electronic textbooks and their compliance with sanitary
norms and regulations as well as on pros and contras of
electronic textbooks usage.
General Integrated Representations оf Technogenic Accidents Danger Indicators
Authors: Киреев  , Kireev V. , Гуменюк  , Gumenyuk V. , Карнюшкин  , Karnyushkin A. , Кармишин  , Karmishin A. , Резничек  , Reznichek V. 
Published: January 2014

The questions of justification of total and integral spatial-temporal hazard indices related to industrial disasters are
considered from the perspective of qualimetry. The problem definition about casual time of destruction effects occurrence
on all square of disaster striking action is formulated. General integral solution of formulated task is given. It is shown,
that general integral expressions related to total and integral spatial-temporal hazard indices of industrial disaster follow
from this solution. Particular cases of disaster’s spatial-temporal indices are considered. For the first time the disaster’s
integral temporal indices such as reduced time of injury effect occurrence and reduced time of retention of injury effect are
proved. Practically relevant tasks, which have to be solved with the use of proved hazard indices of industrial disasters, are
formulated so that the hazard can be reduced to the allowed (psychologically acceptable) level.
In Memoriam: Professor Zbigniew Witold ENGEL 1933 – 2013
Authors: Adamczyk Jan , Batko Wojciech 
Published: January 2014

Abstract
When we look at the history of AGH – University of Science and Technology, recognized as one of the best universities in the country, we find that for that success many people worked for several dozen years. However, the subjects, the methodology and directions of their work were shaped by units – outstanding personalities of science. Just to mention, among others Professors Stanisław Zuber, Władysław Takliński, Witold Budryk, Maksymilian Tytus Huber and Władysław Bogusz. These people have shaped our institution, marked its new path of development and permanent place in the history of Polish and world science. To belong to such a group is a great ennoblement, for which one works out for his entire life. Undoubtedly, such personality was also Professor Zbigniew Witold Engel, to whom we said goodbye on November, 6th, 2013, in the St. Clement’s church in Wieliczka. Professor was born on April 1, 1933, in Zawady near Zhovkva in the province of Lviv. After graduating from Jan Matejko Middle and High School in Wieliczka in 1950 earned a matriculation certificate. Then he began his studies at the Department of Communications of Polytechnic Departments of Academy of Mining and Metallurgy and involved with our university for the next 63 years. October 1, 1952 the Department of Mechanics of the University of Mining and Metallurgy was created, and soon after began professor’s adventure that was the work of science, education and organization. The history and fate of our university are inextricably linked with the person of professor who practically since the very beginning of the Faculty supported it in all areas of its activities, and has always actively participated in academic life.
Speech Emotion Recognition under White Noise
Authors: Huang Chengwei , Chen Guoming , Yu Hua , Bao Yongqiang , Zhao Li 
Published: January 2014

Abstract
Speaker‘s emotional states are recognized from speech signal with Additive white Gaussian noise (AWGN). The influence of white noise on a typical emotion recogniztion system is studied. The emotion classifier is implemented with Gaussian mixture model (GMM). A Chinese speech emotion database is used for training and testing, which includes nine emotion classes (e.g. happiness, sadness, anger, surprise, fear, anxiety, hesitation, confidence and neutral state). Two speech enhancement algorithms are introduced for improved emotion classification. In the experiments, the Gaussian mixture model is trained on the clean speech data, while tested under AWGN with various signal to noise ratios (SNRs). The emotion class model and the dimension space model are both adopted for the evaluation of the emotion recognition system. Regarding the emotion class model, the nine emotion classes are classified. Considering the dimension space model, the arousal dimension and the valence dimension are classified into positive regions or negative regions. The experimental results show that the speech enhancement algorithms constantly improve the performance of our emotion recognition system under various SNRs, and the positive emotions are more likely to be miss-classified as negative emotions under white noise environment.
Controllability-Oriented Placement of Actuators for Active Noise-Vibration Control of Rectangular Plates Using a Memetic Algorithm
Authors: Wrona Stanisław , Pawełczyk Marek 
Published: January 2014

Abstract
For successful active control with a vibrating plate it is essential to appropriately place actuators. One of the most important criteria is to make the system controllable, so any control objectives can be achieved. In this paper the controllability-oriented placement of actuators is undertaken. First, a theoretical model of a fully clamped rectangular plate is obtained. Optimization criterion based on maximization of controllability of the system is developed. The memetic algorithm is used to find the optimal solution. Obtained results are compared with those obtained by the evolutionary algorithm. The configuration is also validated experimentally.
Technical Note. Latest Developments in International Standardization of Whole-Body and Hand-Arm Vibration
Authors: Liedtke Martin , Rissler Jörg , Kaulbars Uwe 
Published: January 2014

Abstract
Latest developments in international standardization of whole-body and hand-arm vibration are pre- sented. In addition, two German projects are presented that might have impact on international work programs in the next years.
Career Planning as a Building Block for Personal Excellence
Authors: Mikačić Marija Turnšek , Ovsenik Marija 
Published: January 2014

Abstract
The narrow field of research here is the development of a model for the building of personal excellence using a career plan that is empirically tested and confirmed, both qualitatively and quantitatively. The purpose of this study relates to the writing of a career plan, for the determination of the factors that influence the feeling of personal excellence of participants in career planning education, in relation to other participants who are not. The qualitative analysis consists of basic experience data that was collected during the education process, through 20 essays, and the paradigmatic model, with the final theory defined, and based on this, a questionnaire was created. The quantitative analysis involved a total of 547 participants. For the statistical analyses of the data, bivariate analysis was used to assess the linear connection of individual pairs of variables. The search for differences between the two groups used t-tests for independent samples. Factor analysis was used to determine whether the relations among the examined variables can be explained with a smaller number of indirectly examined variables. The final model consists of three elements: (1) relation with career; (2) self-esteem; and (3) perception of personal excellence. Management can use the results of this study for management decisions. The increased success of every individual organisation benefits the whole of society. This study represents an original contribution and offers a new approach that is based on the paradigm of the understanding of a career and the importance of its planning as a motive for excellence.
Relation between Entrepreneurial Curiosity and Entrepreneurial Self-efficacy: a Multi-Country Empirical Validation
Authors: Jeraj Mitja , Marič Miha 
Published: January 2014

Abstract
The main purpose of this paper is to present empirical analysis of the relation between entrepreneurial curiosity and entrepreneurial self-efficacy. A detailed literature review in a broad field of entrepreneurship, narrow field of entrepreneurship psychology, and organizational sciences revealed, at one hand different connections between determinants influencing entrepreneurs, and latest scientific research trends on the other hand. Although the significance of curiosity in motivating and learning has received expressive scholarly support, like also entrepreneurial self-efficacy as one of the most studied personal attributes among entrepreneurs, no study to our knowledge existed in relation to entrepreneurial curiosity connected with entrepreneurial self-efficacy. An online multi-country survey was conducted in Slovenia and USA among entrepreneurs and results of structural equation modelling showed that entrepreneurial curiosity and entrepreneurial self-efficacy are related. Entrepreneurial curiosity has a positive impact on entrepreneurial self-efficacy of running entrepreneurial tasks. The findings of this research have both theoretical and practical implications.