bluuebunny/crossref_metadata_2025_split huggingface dataset:
Locked by Design: A Conformationally Constrained Transglutaminase Tag Enables Efficient Site‐Specific Conjugation
Authors: Siegmund Vanessa , Schmelz Stefan , Dickgiesser Stephan , Beck Jan , Ebenig Aileen , Fittler Heiko , Frauendorf Holm , Piater Birgit , Betz Ulrich A. K. , Avrutina Olga , Scrima Andrea , Fuchsbauer Hans‐Lothar , Kolmar Harald 
Published: September 2015

AbstractBased on the crystal structure of a natural protein substrate for microbial transglutaminase, an enzyme that catalyzes protein crosslinking, a recognition motif for site‐specific conjugation was rationally designed. Conformationally locked by an intramolecular disulfide bond, this structural mimic of a native conjugation site ensured efficient conjugation of a reporter cargo to the therapeutic monoclonal antibody cetuximab without erosion of its binding properties.
Electronic Olfactory Sensor Based on A. mellifera Odorant‐Binding Protein 14 on a Reduced Graphene Oxide Field‐Effect Transistor
Authors: Larisika Melanie , Kotlowski Caroline , Steininger Christoph , Mastrogiacomo Rosa , Pelosi Paolo , Schütz Stefan , Peteu Serban F. , Kleber Christoph , Reiner‐Rozman Ciril , Nowak Christoph , Knoll Wolfgang 
Published: September 2015

AbstractAn olfactory biosensor based on a reduced graphene oxide (rGO) field‐effect transistor (FET), functionalized by the odorant‐binding protein 14 (OBP14) from the honey bee (Apis mellifera) has been designed for the in situ and real‐time monitoring of a broad spectrum of odorants in aqueous solutions known to be attractants for bees. The electrical measurements of the binding of all tested odorants are shown to follow the Langmuir model for ligand–receptor interactions. The results demonstrate that OBP14 is able to bind odorants even after immobilization on rGO and can discriminate between ligands binding within a range of dissociation constants from Kd=4 μM to Kd=3.3 mM. The strongest ligands, such as homovanillic acid, eugenol, and methyl vanillate all contain a hydroxy group which is apparently important for the strong interaction with the protein.
Designing Light‐Activated Charge‐Separating Proteins with a Naphthoquinone Amino Acid
Authors: Lichtenstein Bruce R. , Bialas Chris , Cerda José F. , Fry Bryan A. , Dutton P. Leslie , Moser Christopher C. 
Published: September 2015

AbstractThe first principles design of manmade redox‐protein maquettes is used to clarify the physical/chemical engineering supporting the mechanisms of natural enzymes with a view to recapitulate and surpass natural performance. Herein, we use intein‐based protein semisynthesis to pair a synthetic naphthoquinone amino acid (Naq) with histidine‐ligated photoactive metal–tetrapyrrole cofactors, creating a 100 μs photochemical charge separation unit akin to photosynthetic reaction centers. By using propargyl groups to protect the redox‐active para‐quinone during synthesis and assembly while permitting selective activation, we gain the ability to employ the quinone amino acid redox cofactor with the full set of natural amino acids in protein design. Direct anchoring of quinone to the protein backbone permits secure and adaptable control of intraprotein electron‐tunneling distances and rates.
Detection of a New Piperideine Alkaloid in the Pygidial Glands of Some Stenus Beetles
Authors: Wittmann Isabel , Schierling Andreas , Dettner Konrad , Göhl Matthias , Schmidt Jürgen , Seifert Karlheinz 
Published: September 2015

AbstractRove beetles of the genus Stenus produce and store bioactive alkaloids like stenusine (3), 3‐(2‐methylbut‐1‐enyl)pyridine (4), and cicindeloine (5) in their pygidial glands to protect themselves from predation and microorganismic infestation.The biosynthesis of stenusine (3), 3‐(2‐methylbut‐1‐enyl)pyridine (4), and cicindeloine (5) was previously investigated in Stenus bimaculatus, Stenus similis, and Stenus solutus, respectively. The piperideine alkaloid cicindeloine (5) occurs also as a major compound in the pygidial gland secretion of Stenus cicindeloides. The three metabolites follow the same biosynthetic pathway, where the N‐heterocyclic ring is derived from L‐lysine and the side chain from L‐isoleucine. The different alkaloids are finally obtained by few modifications of shared precursor molecules, such as 2,3,4,5‐tetrahydro‐5‐(2‐methylbutylidene)pyridine (1). This piperideine alkaloid was synthesized and detected by GC/MS and GC at a chiral phase in the pygidial glands of Stenus similis, Stenus tarsalis, and Stenus cicindeloides.
Mitsunobu Alkylation of Cancerostatic 5‐Fluorouridine with (2E)‐10‐Hydroxydec‐2‐enoic Acid, a Fatty Acid from Royal Jelly with Multiple Biological Activities
Authors: Ottenhaus Vanessa , Rosemeyer Helmut 
Published: September 2015

Abstract5‐Fluorouridine (1) – a nucleoside antimetabolite with strong cancerostatic properties – was protected i) at the 2′‐ and 3′‐OH groups with a heptan‐4‐ylidene residue and ii) at the 5′‐OH group with a (4‐methoxyphenyl)(diphenyl)methyl residue. This fully protected compound, 3, was submitted to a Mitsunobu reaction with the N‐hydroxysuccinimide (NHS) ester, 5, of (2E)‐10‐hydroxydec‐2‐enoic acid (4) which gave nucleolipid 6. The latter was detritylated with Cl2CHCOOH to yield the co‐drug 7 as NHS ester.
Stereospecific Synthesis of α‐ and β‐Hydroxyalkyl P‐Stereogenic Phosphine–Boranes and Functionalized Derivatives: Evidence of the PO Activation in the BH3‐Mediated Reduction
Authors: Lemouzy Sébastien , Nguyen Duc Hanh , Camy Valentine , Jean Marion , Gatineau David , Giordano Laurent , Naubron Jean‐Valère , Vanthuyne Nicolas , Hérault Damien , Buono Gérard 
Published: September 2015

AbstractAccess to hydroxy‐functionalized P‐chiral phosphine–boranes has become an important field in the synthesis of P‐stereogenic compounds used as ligands in asymmetric catalysis. A family of optically pure α and β‐hydroxyalkyl tertiary phosphine–boranes has been prepared by using a three‐step procedure from readily accessible enantiopure adamantylphosphinate, obtained by semi‐preparative HPLC on multigram scale. Firstly, a two‐step one‐pot transformation affords the enantiopure hydroxyalkyl tertiary phosphine oxides in good yields and enantioselectivities. The third step, BH3‐mediated reduction, allows the formation of the desired phosphine–boranes with excellent stereospecifity. The mechanistic study of this reduction provides new evidence to elucidate the crucial role of the pendant hydroxy group and the subsequent activation of the PO bond by the boron atom.
Lithium Bromide/Water as Additives in Dearomatizing Samarium‐Ketyl (Hetero)Arene Cyclizations
Authors: Rao Chintada Nageswara , Bentz Christoph , Reissig Hans‐Ulrich 
Published: September 2015

AbstractNew conditions for dearomatizing samarium‐ketyl (hetero)arene cyclizations are reported. In many examples of these samarium diiodide‐mediated reactions, lithium bromide and water can be used as additives instead of the carcinogenic and mutagenic hexamethylphosphoramide (HMPA). The best results were obtained for the cyclizations of N‐acylated indole derivatives delivering the expected indolines in good yields and excellent diastereoselectivities. A new type of cyclization delivering indolyl‐substituted allene derivatives is also described. The scope and limitations of the lithium bromide/water system are discussed.
Ruthenium‐Immobilized Periodic Mesoporous Organosilica: Synthesis, Characterization, and Catalytic Application for Selective Oxidation of Alkanes
Authors: Ishito Nobuhiro , Kobayashi Hirokazu , Nakajima Kiyotaka , Maegawa Yoshifumi , Inagaki Shinji , Hara Kenji , Fukuoka Atsushi 
Published: September 2015

AbstractInvited for the cover of this issue is the group of Kenji Hara and Atsushi Fukuoka at Hokkaido University (Japan). The image depicts the catalytic oxidation of alkanes over isolated Ru complexes formed on periodic mesoporous organosilica. Read the full text of the article at 10.1002/chem.201502638.
CFD simulation and experimental study on vapour splitter in packed Divided Wall Column
Authors: Sun Jiao , Ge Huaqiang , Chen Wenyi , Chen Nan , Chen Xiangwu 
Published: September 2015

AbstractA Divided Wall Column (DWC) with an optimal vapour split offers much greater potential for energy savings compared to conventional column sequences. To realize the adjustment of the vapour split as well as uniform vapour distribution, a vapour splitter which had been applied for an invention patent was put forward. The effects on performance of adjusting the vapour split and gas nonuniformities under different factors were investigated using numerical simulation and experimental research. The simulation study was carried out using the Computational Fluid Dynamics (CFD) software STAR‐CCM+. The simulation results were compared with experimental data, and they showed close agreement with each other. All the results revealed that the device can flexibly adjust the vapour split and achieve a uniform vapour distribution.
A Fluorescence‐Based Screening Protocol for the Identification of Water Oxidation Catalysts
Authors: Detz Remko J. , Abiri Zohar , Kluwer Alexander M. , Reek Joost N. H. 
Published: September 2015

AbstractInvited for this month’s cover is the group of Joost Reek at the University of Amsterdam. The image shows a screening approach for the identification of molecular catalysts for chemical oxidation of water, which is based on oxygen‐sensitive fluorescence‐quenching using an OxoDish. The Full Paper itself is available at 10.1002/cssc.201500558.
Investigations of the Thermal Responsiveness of 1,4,2‐Oxathiazoles
Authors: Hewitt Russell J. , Ong Michelle Jui Hsien , Lim Yi Wee , Burkett Brendan A. 
Published: September 2015

AbstractThe first systematic study of the thermal rearrangement/fragmentation of 5,5‐disubstituted 1,4,2‐oxathiazoles into isothiocyanates is reported. Structure–activity relationships reveal that the choice of substituent at the 5‐position of the 1,4,2‐oxathiazoles is the predominant factor to influence the ease of fragmentation.
Two New Iridoid Esters from the Root and Rhizome of Valeriana jatamansiJones
Authors: Yang Bo , Zhang Jian‐Fen , Song Hui‐Zhu , Gu Man‐Cang , Zhao Hua‐Jun , Xiong Yao‐Kang 
Published: September 2015

AbstractTwo new iridoid esters, named valerjatadoids A and B (1 and 2, resp.), together with three known iridoid esters, jatamanin O (3), jatamanvaltrate P (4), and jatamanvaltrate Q (5), have been isolated from the root and rhizome of Valeriana jatamansi Jones. The structures of the two new compounds were elucidated by spectroscopic analyses, including 2D‐NMR techniques.
Reaction between Furan‐ or Thiophene‐2‐carbonyl Chloride, Isocyanides, and Dialkyl Acetylenedicarboxy­lates: Multicomponent Synthesis of 2,2′‐Bifurans and 2‐(Thiophen‐2‐yl)furans
Authors: Sayahi Mohammad Hosein , Adib Mehdi , Hamooleh Zeinab , Zhu Long‐Guan , Amanlou Massoud 
Published: September 2015

AbstractAn efficient multi‐component synthesis of highly functionalized 2,2′‐bifurans and 2‐(thiophen‐2‐yl)furans is described. A mixture of furan‐ or thiophene‐2‐carbonyl chloride, an isocyanide, and a dialkyl acetylenedicarboxylate undergoes a smooth addition reaction in dry CH2Cl2 at ambient temperature to produce 2‐amino‐5‐(4‐chlorofuran‐2‐yl)furan‐3,4‐dicarboxylates and 2‐amino‐5‐(4‐chlorothiophen‐2‐yl)furan‐3,4‐dicarboxylates. A single‐crystal X‐ray‐analysis of a derivative conclusively confirms the structure of these 2,2′‐bifurans and 2‐(thiophen‐2‐yl)furans. A novel electrophilic aromatic substitution reaction can justify the formation of the Cl‐substituted furan or thiophene rings.
Concise Synthesis of 2‐Arylpropanoic Acids and Study of Unprecedented Reduction of 3‐Hydroxy‐2‐arylpropenoic Acid Ethyl Ester to 2‐Arylpropenoic Acid Ethyl Ester by BH3⋅THF
Authors: Shahid Islam M. , Ahmad Syarhabil , Attu Mary Rose , Foerstering F. Holger , Mahmun Hossain M. 
Published: September 2015

AbstractWe have developed a concise method of synthesizing racemic arylpropanoic acids, which have been widely used as nonsteroidal anti‐inflammatory drugs (NSAIDs). The synthesis involves only four steps from commercially available benzaldehyde. The synthesis incorporates an unprecedented reduction reaction, conversion of 3‐hydroxy‐2‐arylpropenoic acid ethyl ester to 2‐arylpropenoic acid ethyl ester by BH3⋅THF. The reduction reaction has been investigated and optimized.
New Monoterpenoids from Fruits of Gardenia jasminoides var. radicans
Authors: Yu Shao‐Fu , Huang Xiao‐Jun , Fu Shu‐Na , Wu Chun , Ye Wen‐Cai , Zhou Guang‐Xiong 
Published: September 2015

AbstractOne new lactone, cyclopentanepyrone A (1), and two new monoterpenoids, gardeterpenone A (2) and jasminoside V (3), were isolated from the fruits of Gardenia jasminoides var. radicans, along with four known monoterpenoids, 4–7, which were isolated from this plant for the first time. The structures of the isolates were elucidated by extensive spectroscopic studies, including UV, IR, 1D‐ and 2D‐NMR, ESI‐MS, HR‐ESI‐MS, and CD experiments.
Atom‐Economical Synthesis of Complex Heterocycles with NO Moiety
Authors: Merey Gökçe , Sezen Gökhan 
Published: September 2015

AbstractA concise and facile method for the synthesis of heterocyclic compounds with NO tether was introduced. The two important steps of the synthesis are a Mitsunobu reaction and CH activation. The Mitsunobu protocol allows to form the NO moiety in the molecule, while subsequent CH activation leads to heterocycles.
Effects of pre‐Sowing Irrigation on Crop Water Consumption, Grain Yield and Water Productivity of Winter Wheat in the North China Plain
Authors: Gao Yang , Shen Xiaojun , Li Xinqiang , Meng Zhaojiang , Sun Jingsheng , Duan Aiwang 
Published: September 2015

AbstractIn three growing seasons from 2009 to 2012, we investigated the effects of different combinations of pre‐sowing irrigation and supplemental irrigation on crop water consumption, grain yield, and water productivity (WP) of winter wheat. One conventional irrigation schedule (irrigation applied at the overwintering and jointing stages) can stabilize wheat yield and WP with low or sufficient rainfall, but another conventional irrigation schedule (irrigation applied at the reviving and filling stages) decreased wheat yield and WP significantly with continuous droughts throughout winter and spring. The winter wheat with the pre‐sowing irrigation and supplemental irrigation applied at the recovery or elongation stage had a grain yield and WP in the seasons with less rainfall during the early growing stage that was higher than 7500 kg ha‐1 and 1.85 kg m‐3, respectively. In contrast, the irrigation schedule with the pre‐sowing irrigation and irrigation applied at the grain‐filling stage decreased wheat yield and WP significantly in the seasons with continuous droughts throughout winter and spring. These results indicated that combined with pre‐sowing irrigation, supplemental irrigation should be applied at the recovery stage and not later than the jointing stage; otherwise, the grain yield and WP of winter wheat will be decreased. Copyright © 2015 John Wiley & Sons, Ltd.
PLZF‐Induced Upregulation of CXCR4 Promotes Dairy Goat Male Germline Stem Cell Proliferation by Targeting Mir146a
Authors: Mu Hailong , Li Na , Wu Jiang , Zheng Liming , Zhai Yuanxin , Li Bo , Song Wencong , Wang Jinglu , Zhu Haijing , Li Guangpeng , Hua Jinlian 
Published: September 2015

ABSTRACTPrevious studies have shown that promyelocytic leukemia zinc finger (PLZF), chemokine (C‐X‐C motif) receptor 4 (CXCR4) and mir146a were associated with the self‐renewal of mouse spermatogonial stem cells (SSCs); however, there is little information on their effects on the fate of livestock SSCs. Here, we have identified a regulatory pathway in dairy goat mGSCs, involving PLZF, mir146a and the SDF‐1 receptor CXCR4. PLZF overexpression downregulated mir146a and simultaneously upregulated the expression of CXCR4 protein, whereas PLZF knockdown (siPLZF) induced the specifically opposite effects. The in vitro assays demonstrated that PLZF specifically interacts with and suppresses the mir146a promoter, and mir146a targets CXCR4 to impede its translation. The levels of ERK1/2 phosphorylation in the mGSCs overexpressed CXCR4 and PLZF were upregulated, respectively, whereas mir146a expression was decreased and CXCR4 protein was increased. Mir146a overexpression and siPLZF impaired mGSC proliferation and differentiation, however, Mir146a knockdown induced the opposite effects. The effects of PLZF and mir146a were mediated regulation by mir146a and CXCR4, respectively. Overexpression of CXCR4 or addition of CXCL12 in cultures of dairy goat mGSCs resulted in the upregulation of their signaling, and the phosphorylation of ERK1/2 was increased. Collectively, these findings indicate that PLZF is an important transcription factor in the regulation of the expression of CXCR4 to promote dairy goat mGSC proliferation by targeting mir146a. J. Cell. Biochem. 117: 844–852, 2016. © 2015 Wiley Periodicals, Inc.
Noninvasive measurement of intracranial pressure via the pulsatility index on transcranial doppler sonography: Is improvement possible?
Authors: Morgalla Matthias H. , Magunia Harry 
Published: September 2015

ABSTRACTPurposeWe hypothesized that using hemodynamic variables could improve the prediction of intracranial pressure (ICP) from the middle cerebral artery pulsatility index (PI) measured with transcranial Doppler sonography.MethodsIn this prospective study, 39 patients with traumatic brain injury were routinely examined with transcranial Doppler sonography, and the middle cerebral artery PI was calculated. A multivariate model including hematocrit, mean arterial blood pressure, heart rate, and arterial CO2 pressure (PaCO2) was evaluated.ResultsThirty‐nine comatose patients (16 women and 23 men; age range 18–73 years; median 44 years) were included, and 234 data pairs (consisting of ICP and corresponding PI values) were analyzed. ICP ranged from −3 mmHg to +52 mmHg, and PI from 0.6 to 2.85. We found a significant but weak correlation between PI and the square root of ICP (R2 between 0.29 and 0.34, p < 0.0001). A slightly stronger correlation was detected when hemodynamic variables were incorporated (R2 between 0.37 and 0.43). Of these variables, mean arterial blood pressure had the most significant influence.ConclusionsIn this study, PI was not a sufficiently strong predictor of ICP to be used in clinical practice. Its reliability did not improve even when hemodynamic variables were considered. Therefore, we recommend abandoning the use of PI for the noninvasive measurement of ICP in clinical practice. © 2015 Wiley Periodicals, Inc. J Clin Ultrasound 44:40–45, 2016
Improvement of alignment quality for FLC with mixing liquid crystal polymer
Authors: Yao Lishuang , Tang Xianzhu , Peng Zenghui , Xuan Li 
Published: September 2015

AbstractFerroelectric liquid crystal (FLC) with I‐N*‐C* phase sequence is most attractive due to its continuous grey level and fast response; however, the alignment problem of two‐domains defect restricted its application. In this work, one kind of one terminal polymerizable nematic liquid crystal (NLC) mesogen was mixed to FLC to improve its alignment quality. Experimental results showed that mono‐domain uniform alignment of FLC can be obtained with mixing NLC polymer. With optimized concentration, the FLC devices could offer half‐V‐shaped electric‐optical characteristic of driving contrast of 182:1 and fast response of 300 µs even after polymerization. This work can provide one simple and effective method for fabricating stable I‐N*‐C* FLC devices.
Effects of overflights on incubating Wilson's plover behavior and heart rate
Authors: Derose‐Wilson Audrey , Fraser James D. , Karpanty Sarah M. , Hillman Matthew D. 
Published: September 2015

ABSTRACTIncreasing attention is paid to the effects of human activities, including the use of aircraft, on wildlife. However, responses to visual and auditory stimuli associated with aircraft are highly species‐ and context‐dependent and results of existing studies should not be generalized across species, or even across life stages of the same species. We used digital audio recorders, auto‐triggered cameras, and heart rate monitors to study the responses of nesting Wilson's plovers (Charadrius wilsonia) to different types of planes (civilian and military fixed‐wing aircraft) and helicopters (civilian and military rotary‐wing aircraft) at Cape Lookout National Seashore, North Carolina. We compared vigilance behavior, incubation rate, and heart rate before, during, and after overflights. Wilson's plovers were alert and scanned more during military rotary‐wing overflights, and scanned more during both military and civilian fixed‐wing overflights than during periods without overflights. Heart rates and incubation rates did not change during any type of overflights. Although we found no direct link between increased vigilance rates and decreased reproductive success, a substantial increase in aircraft traffic at our study site, especially rotary‐wing overflights, would merit further investigation. © 2015 The Wildlife Society.
Strong deshielding in aromatic isoxazolines
Authors: Ungvarská Maľučká Lucia , Vilková Mária , Kožíšek Jozef , Imrich Ján 
Published: September 2015

AbstractVery strong proton deshielding was found in di/tri‐aromatic isoxazoline regioisomers prepared from acridin‐4‐yl dipolarophiles and stable benzonitrile oxides (BNO). Three alkenes, (acridin‐4‐yl)‐CHCH‐R (R = COOCH3, Ph, and CONH2), reacted with three BNO dipoles (2,4,6‐trimethoxy, 2,4,6‐trimethyl, 2,6‐dichloro) to give pairs of target isoxazolines with acridine bound to C‐4 or C‐5 carbon of the isoxazoline (denoted as 4‐Acr or 5‐Acr). Regioselectivity was dependent on both the dipolarophile and dipole character. The ester and amide dipolarophile displayed variable regioselectivity in cycloadditions whereas the styrene one afforded prevailing 4‐Acr regioisomers. 2,4,6‐Trimethoxy‐BNO was most prone to form 5‐Acr isoxazolines while mesitonitrile oxide gave major 4‐Acr isoxazolines. Basic hydrolysis of the amide cycloadduct led to an unexpected isoxazolone product. The structure of the target compounds was studied by NMR, MS, and X‐ray crystallography. Copyright © 2015 John Wiley & Sons, Ltd.
New DVB‐S2X constellations for improved performance on the satellite channel
Authors: Eroz Mustafa , Lee Lin‐Nan , Loghin Nabil , De Bie Ulrik , Simoens Frederik , Delaruelle Daniel 
Published: September 2015

SummaryDigital Video Broadcasting via satellite second generation has experienced worldwide adoption because of its revolutionary and yet practical physical layer technology and its flexibility. Recently, the standard has been updated with several new features without changing its fundamental structure. This paper provides a high‐level discussion on several of the most important additions to the new standard with particular emphasis on some of the new signal constellations. Copyright © 2015 John Wiley & Sons, Ltd.