bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of treading by two breeds of dairy cow with different live weights on soil physical properties, poaching damage and herbage production on a poorly drained clay-loam soil
Authors: TUOHY P. , FENTON O. , HOLDEN N. M. , HUMPHREYS J. 
Published: October 2014

SUMMARYThere is little empirical evidence to indicate that dairy cow live weight affects the extent of soil damage at the hoof-soil interface during grazing on poorly drained permanent grassland. In the present study the impact of Holstein-Friesian (HF) dairy cows with a mean (±standard deviation) live weight of 570 (±61) kg were compared with Jersey × Holstein-Friesian (JX) with a mean live weight of 499 (±52) kg each at two stocking densities: mean 2·42 ± (0·062) and 2·66 (±0·079) cows/ha. Soil physical properties (bulk density, macroporosity, gravimetric water content, air-filled porosity, penetration resistance and shear strength), poaching damage (post-grazing soil surface deformation and hoof-print depth), herbage yield and milk production were measured throughout 2011 and 2012. Soil physical properties, post-grazing soil surface deformation and herbage production were not affected by dairy cow breed or by interactions between breed and stocking density. Hoof-print depth was higher in the HF treatments (39 v. 37 mm, s.e. 0·5 mm). Loading pressure imposed at the soil surface was the same for both breeds due to a direct correlation between live weight and hoof size. Poaching damage was greater at higher stocking density. Using the lighter JX cow offered little advantage in terms of lowering the negative impact of treading on soil physical properties or reducing poaching damage and no advantage in terms of herbage or milk production compared with the heavier HF cow.
Molecular characterization of putative parasitism genes in the plant-parasitic nematode Meloidogyne hispanica
Authors: Duarte A. , Maleita C. , Tiago I. , Curtis R. , Abrantes I. 
Published: October 2014

AbstractMeloidogyne hispanica (Mhi) is a difficult-to-control polyphagous root-knot nematode (RKN) species of emerging importance for economically valuable crops. Nematode secretions are likely to be the first signals perceived by the plant and are thought to be involved in various aspects of the plant–nematode interaction. The aims of this work were to identify and characterize M. hispanica parasitism genes: cathepsin L cysteine protease (cpl-1), calreticulin (crt-1), β-1,4-endoglucanase-1 (eng-1) and manganese superoxide dismutase (mnsod). As there are no genomic data available for M. hispanica, primers were designed from the conserved regions of the putative parasitism genes in M.incognita and M. hapla and used to amplify the genes in M. hispanica, which led to the successful amplification of these genes in M. hispanica. Partial gene sequences were also obtained for M. arenaria, M. hapla, M. hispanica, M. incognita and M. javanicacpl-1, crt-1, eng-1 and mnsod genes, and their phylogenetic relationship analysed. In order to determine whether these genes are differentially expressed during M. hispanica development, cDNA was amplified from mRNA isolated from eggs, second-stage juveniles (J2) and females. Amplification products were observed from cDNA of all developmental stages for the Mhi-cpl-1 and Mhi-crt-1 genes. However, the gene Mhi-crt-1 exhibited intense amplification bands in females, while the Mhi-eng-1 gene was equally amplified in eggs and J2 and the Mhi-mnsod gene was only expressed in eggs. In comparison to the other RKN species, the genes Mhi-eng-1 and Mhi-mnsod showed transcription in different nematode developmental stages.
Treatment of vertigo and postural instability using visual illusions
Authors: van Kerckhoven G , Mert A , De Ru J A 
Published: October 2014

AbstractBackground:Ototoxicity caused by medication can lead to debilitating symptoms such as dizziness, vertigo and postural instability. There is no current ‘gold standard’ treatment available.Case report:A 79-year-old male, with bilateral loss of vestibular function caused by gentamicin toxicity after surgery for prosthetic valve endocarditis, complained of dizziness, difficulty in walking and an increased risk of falling. Physical examination showed a positive head thrust test suggesting bilateral loss of vestibular function.Results:The patient underwent a specific motion-based virtual reality enhanced protocol for peripheral vestibular disease. He showed a great improvement, with a 50 per cent reduction in his Dizziness Handicap Inventory score.Conclusion:Computer-aided rehabilitation programmes might represent an important advance in gait and posture training.
Effects of livestock on guanacoLama guanicoedensity, movements and habitat selection in a forest–grassland mosaic in Tierra del Fuego, Chile
Authors: Moraga Claudio A. , Funes Martín C. , Pizarro J. Cristóbal , Briceño Cristóbal , Novaro Andrés J. 
Published: October 2014

AbstractLocally abundant ungulates often come into conflict with human activities. After a population collapse that reached its nadir in the 1970s, the guanacoLama guanicoepopulation in Tierra del Fuego, Chile, recovered and is now in conflict with sheep ranching and commercial logging. We studied the effects of livestock density and environmental factors on guanaco abundance and spatial ecology, using seasonal counts and radio-telemetry in a private protected area (Karukinka) and neighbouring ranches in a forest–grassland mosaic in Tierra del Fuego. Guanaco density was highest in low-elevation areas with more grassland cover and little snow accumulation in winter. In low-elevation areas, guanaco density decreased with increasing livestock density. Radio-tracked guanacos exhibited a partial migration pattern: two individuals migrated seasonally, selecting grasslands and avoiding forests mainly in summer, whereas six sedentary individuals used habitats according to their availability. Migratory guanacos spent the summer in Karukinka and winter on nearby ranches. High sheep densities and poor range condition on the ranches reduce key forage resources available to guanacos and may promote use of forests by guanacos, affecting forest regeneration and increasing conflict with logging. Current guanaco harvest by loggers may fail to reduce the impact of guanacos on logged-forest regeneration if guanaco spatial ecology and sheep management are not considered. Our results provide insight into the interactions among guanacos, forests and livestock ranching, and may be used to reduce conflicts and guide conservation in the Fuegian ecosystem.
Corticostriatothalamic reward prediction error signals and executive control in late-life depression
Authors: Dombrovski A. Y. , Szanto K. , Clark L. , Aizenstein H. J. , Chase H. W. , Reynolds C. F. , Siegle G. J. 
Published: October 2014

BackgroundAltered corticostriatothalamic encoding of reinforcement is a core feature of depression. Here we examine reinforcement learning in late-life depression in the theoretical framework of the vascular depression hypothesis. This hypothesis attributes the co-occurrence of late-life depression and poor executive control to prefrontal/cingulate disconnection by vascular lesions.MethodOur fMRI study compared 31 patients aged ⩾60 years with major depression to 16 controls. Using a computational model, we estimated neural and behavioral responses to reinforcement in an uncertain, changing environment (probabilistic reversal learning).ResultsPoor executive control and depression each explained distinct variance in corticostriatothalamic response to unexpected rewards. Depression, but not poor executive control, predicted disrupted functional connectivity between the striatum and prefrontal cortex. White-matter hyperintensities predicted diminished corticostriatothalamic responses to reinforcement, but did not mediate effects of depression or executive control. In two independent samples, poor executive control predicted a failure to persist with rewarded actions, an effect distinct from depressive oversensitivity to punishment. The findings were unchanged in a subsample of participants with vascular disease. Results were robust to effects of confounders including psychiatric comorbidities, physical illness, depressive severity, and psychotropic exposure.ConclusionsContrary to the predictions of the vascular depression hypothesis, altered encoding of rewards in late-life depression is dissociable from impaired contingency learning associated with poor executive control. Functional connectivity and behavioral analyses point to a disruption of ascending mesostriatocortical reward signals in late-life depression and a failure of cortical contingency encoding in elderly with poor executive control.
Generation of monoenergetic proton beams by a combined scheme with an overdense hydrocarbon target and an underdense plasma gas irradiated by ultra-intense laser pulse
Authors: Yao Weipeng , Li Baiwen , Cao Lihua , Zheng Fanglan , Huang Taiwu , Xiao Chengzhuo , Skoric Milos M. 
Published: October 2014

AbstractAn optimization scheme for the generation of monoenergetic proton beams by using an overdense hydrocarbon target, followed by an underdense plasma gas, irradiated by an ultra-intense laser pulse is presented. The scheme is based on a combination of a radiation pressure acceleration mechanism and a laser wakefield acceleration mechanism, and is verified by one-dimensional relativistic particle-in-cell (1D PIC) simulations. As compared to the pure hydrogen (H) target, protons in the hydrocarbon target can be pre-accelerated to higher energy and compressed in space due to the existence of the heavy carbon atoms, which provides a better injection process for the successive laser wakefield acceleration in the underdense plasma gas, resulting in the generation of a monoenergetic, tens-of-GeV proton beam. Additionally, for the first time, it is found that the use of the hydrocarbon target can reduce the requirement for laser intensity to generate proton beams with the same energy in this combined scheme, as compared to the use of the pure H target.
A caterpillar climbing robot with spine claws and compliant structural modules
Authors: Wang Wei , Wu Shilin 
Published: October 2014

SUMMARYThis paper proposes a modular caterpillar climbing robot using spines as the attaching tools. To improve the reliability of the spines' engagement and disengagement, this paper discusses the reasonable trajectory of the spine and designs a driving mechanism of the spine based on the compliant mechanism theory. Then some compliant modules are designed and realized to build the caterpillar climbing robot. A climbing gait is designed to avoid collisions between the spines and the wall, and allows the robot to climb on a stucco-like wall with a 72○ incline. The real tests reveal that the deformation of the compliant toes reduces the sliding forces between the spines and the wall, and improve the climbing action obviously.
Expression and cellular distribution of estrogen and progesterone receptors and the real-time proliferation of porcine cumulus cells
Authors: Kempisty Bartosz , Ziółkowska Agnieszka , Ciesiółka Sylwia , Piotrowska Hanna , Antosik Paweł , Bukowska Dorota , Brüssow Klaus P. , Nowicki Michał , Zabel Maciej 
Published: October 2014

SummaryAlthough the expression of estrogen and progesterone receptors within porcine ovary and cumulus–oocyte complexes (COCs) is well recognized, still little information is known regarding expression of the progesterone receptor (PGR), PGR membrane component 1 (PGRMC1) and of estrogen-related receptors (ERRγ and ERRβ/γ) in separated cumulus cells in relation to real-time proliferation. In this study, a model of oocytes-separated cumulus cells was used to analyze the cell proliferation index and the expression PGR, PGRMC1 and of ERRγ and ERRβ/γ during 96-h cultivation in vitro using real-time quantitative PCR (qRT-PCR) and confocal microscopic observation. We found that PGR protein expression was increased at 0 h, compared with PGR protein expression after 96 h of culture (P < 0.001). The expression of PGRMC1, ERRγ and ERRβ/γ was unchanged. After using qRT-PCR we did not found statistical differences in expression of PGR, PGRMC1, ERRγ and ERRβ/γ during 96 h of cumulus cells in vitro culture (IVC). We supposed that the differential expression of the PGR protein at 0 h and after 96 h is related to a time-dependent down-regulation, which may activate a negative feedback. The distribution of PGR, PGRMC1 proteins may be linked with the translocation of receptors to the cytoplasm after the membrane binding of respective agonists and intra-cytoplasmic signal transduction. Furthermore, cumulus cells analyzed at 0 h were characterized by decreased proliferation index, whereas those after 96 h of culture revealed a significant increase of proliferation index, which may be associated with differentiation/luteinization of these cells during real-time proliferation.
Restricted development of mouse triploid fetuses with disorganized expression of imprinted genes
Authors: Yamazaki Wataru , Takahashi Masashi , Kawahara Manabu 
Published: October 2014

SummaryEukaryotic species commonly contain a diploid complement of chromosomes. The diploid state appears to be advantageous for mammals because it enables sexual reproduction and facilitates genetic recombination. Nonetheless, the effects of DNA ploidy on mammalian ontogeny have yet to be understood. The present study shows phenotypic features and expression patterns of imprinted genes in tripronucleate diandric and digynic triploid (DAT and DGT) mouse fetuses on embryonic day 10.5 (E10.5). Measurement of crown–rump length revealed that the length of DGT fetuses (1.87 ± 0.13 mm; mean ± standard error of the mean) was much smaller than that of diploid fetuses (4.81 ± 0.05 mm). However, no significant difference was observed in the crown–rump length between diploid and DAT fetuses (3.86 ± 0.43 mm). In DGT fetuses, the expression level of paternally expressed genes,Igf2, Dlk1, Ndn, andPeg3, remained significantly reduced and that of maternally expressed genes,Igf2randGrb10, increased. Additionally, in DAT fetuses, theIgf2mRNA expression level was approximately twice that in diploid fetuses, as expected. These results provide the first demonstration that imprinted genes in mouse triploid fetuses show distinctive expression patterns independent of the number of parental-origin haploid sets. These data suggest that both DNA ploidy and asymmetrical functions of parental genomes separately influence mammalian ontogeny.
Chemical dissection of the cell cycle: probes for cell biology and anti-cancer drug development
Authors: Senese S , Lo Y C , Huang D , Zangle T A , Gholkar A A , Robert L , Homet B , Ribas A , Summers M K , Teitell M A , Damoiseaux R , Torres J Z 
Published: October 2014

AbstractCancer cell proliferation relies on the ability of cancer cells to grow, transition through the cell cycle, and divide. To identify novel chemical probes for dissecting the mechanisms governing cell cycle progression and cell division, and for developing new anti-cancer therapeutics, we developed and performed a novel cancer cell-based high-throughput chemical screen for cell cycle modulators. This approach identified novel G1, S, G2, and M-phase specific inhibitors with drug-like properties and diverse chemotypes likely targeting a broad array of processes. We further characterized the M-phase inhibitors and highlight the most potent M-phase inhibitor MI-181, which targets tubulin, inhibits tubulin polymerization, activates the spindle assembly checkpoint, arrests cells in mitosis, and triggers a fast apoptotic cell death. Importantly, MI-181 has broad anti-cancer activity, especially against BRAFV600E melanomas.
Numb-dependent integration of pre-TCR and p53 function in T-cell precursor development
Authors: Martin-Blanco N M , Checquolo S , Del Gaudio F , Palermo R , Franciosa G , Di Marcotullio L , Gulino A , Canelles M , Screpanti I 
Published: October 2014

AbstractNumb asymmetrically segregates at mitosis to control cell fate choices during development. Numb inheritance specifies progenitor over differentiated cell fates, and, paradoxically, also promotes neuronal differentiation, thus indicating that the role of Numb may change during development. Here we report that Numb nuclear localization is restricted to early thymocyte precursors, whereas timed appearance of pre-T-cell receptor (pre-TCR) and activation of protein kinase Cθ promote phosphorylation-dependent Numb nuclear exclusion. Notably, nuclear localization of Numb in early thymocyte precursors favors p53 nuclear stabilization, whereas pre-TCR-dependent Numb nuclear exclusion promotes the p53 downmodulation essential for further differentiation. Accordingly, the persistence of Numb in the nucleus impairs the differentiation and promotes precursor cell death. This study reveals a novel regulatory mechanism for Numb function based on its nucleus–cytosol shuttling, coupling the different roles of Numb with different stages of T-cell development.
MiR-29b controls fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling
Authors: Shin J , Shin Y , Oh S-M , Yang H , Yu W-J , Lee J-P , Huh S-O , Lee S H , Suh Y-H , Chung S , Kim H-S 
Published: October 2014

Abstractβ-Catenin has been widely implicated in the regulation of mammalian development and cellular homeostasis. However, the mechanisms by which Wnt/β-catenin signaling components regulate physiological events during brain development remain undetermined. Inactivation of glycogen synthase kinase (GSK)-3β leads to β-catenin accumulation in the nucleus, where it couples with T-cell factor (TCF), an association that is disrupted by ICAT (inhibitor of β-catenin and T cell factor). In this study, we sought to determine whether regulation of ICAT by members of the microRNA-29 family plays a role during neurogenesis and whether deregulation of ICAT results in defective neurogenesis due to impaired β-catenin-mediated signaling. We found that miR-29b, but not miR-29a or 29c, is significantly upregulated in three-dimensionally cultured neural stem cells (NSCs), whereas ICAT is reduced as aged. Treatment with a miR-29b reduced the reporter activity of a luciferase-ICAT 3′-UTR construct whereas a control (scrambled) miRNA oligonucleotide did not, indicating that miR-29b directly targets the 3′-UTR of ICAT. We also found that treatment with miR-29b diminished NSC self-renewal and proliferation, and controlled their fate, directing their differentiation along certain cell lineages. Furthermore, our in vivo results showed that inhibition of miR-29b by in utero electroporation induced a profound defect in corticogenesis during mouse development. Taken together, our results demonstrate that miR-29b plays a pivotal role in fetal mouse neurogenesis by regulating ICAT-mediated Wnt/β-catenin signaling.
Identification and characterization of the novel Col10a1 regulatory mechanism during chondrocyte hypertrophic differentiation
Authors: Gu J , Lu Y , Li F , Qiao L , Wang Q , Li N , Borgia J A , Deng Y , Lei G , Zheng Q 
Published: October 2014

AbstractThe majority of human skeleton develops through the endochondral pathway, in which cartilage-forming chondrocytes proliferate and enlarge into hypertrophic chondrocytes that eventually undergo apoptosis and are replaced by bone. Although at a terminal differentiation stage, hypertrophic chondrocytes have been implicated as the principal engine of bone growth. Abnormal chondrocyte hypertrophy has been seen in many skeletal dysplasia and osteoarthritis. Meanwhile, as a specific marker of hypertrophic chondrocytes, the type X collagen gene (COL10A1) is also critical for endochondral bone formation, as mutation and alteredCOL10A1expression are often accompanied by abnormal chondrocyte hypertrophy in many skeletal diseases. However, how the type X collagen gene is regulated during chondrocyte hypertrophy has not been fully elucidated. We have recently demonstrated that Runx2 interaction with a 150-bp mouseCol10a1cis-enhancer is required but not sufficient for its hypertrophic chondrocyte-specific reporter expression in transgenic mice, suggesting requirement of additionalCol10a1regulators. In this study, we reportin silicosequence analysis of this 150-bp enhancer and identification of its multiple binding factors, including AP1, MEF2, NFAT, Runx1 and TBX5. Using this enhancer as bait, we performed yeast one-hybrid assay and identified multiple candidateCol10a1-interacting genes, includingcyclooxygenase 1(Cox-1) andCox-2. We have also performed mass spectrometry analysis and detected EF1-alpha, Fus, GdF7 and Runx3 as components of the specific complex formed by the cis-enhancer and nuclear extracts from hypertrophic MCT (mouse chondrocytes immortalized with large T antigen) cells that expressCol10a1abundantly. Notably, some of the candidate genes are differentially expressed in hypertrophic MCT cells and have been associated with chondrocyte hypertrophy and Runx2, an indispensibleCol10a1regulator. Intriguingly, we detected high-level Cox-2 expression in hypertrophic MCT cells. Electrophoretic mobility shift assay and chromatin immunoprecipitation assays confirmed the interaction between Cox-2 andCol10a1cis-enhancer, supporting its role as a candidateCol10a1regulator. Together, our data support a Cox-2-containing, Runx2-centeredCol10a1regulatory mechanism, during chondrocyte hypertrophic differentiation.
Stability of the Medial Olivocochlear Reflex as Measured by Distortion Product Otoacoustic Emissions
Authors: Mishra Srikanta K. , Abdala Carolina 
Published: October 2014

Purpose
The purpose of this study was to assess the repeatability of a fine-resolution, distortion product otoacoustic emission (DPOAE)–based assay of the medial olivocochlear (MOC) reflex in normal-hearing adults.

Method
Data were collected during 36 test sessions from 4 normal-hearing adults to assess short-term stability and 5 normal-hearing adults to assess long-term stability. DPOAE level and phase measurements were recorded with and without contralateral acoustic stimulation. MOC reflex indices were computed by (a) noting contralateral acoustic stimulation-induced changes in DPOAE level (both absolute and normalized) at fine-structure peaks, (b) recording the effect as a vector difference, and (c) separating DPOAE components and considering a component-specific metric.

Results
Analyses indicated good repeatability of all indices of the MOC reflex in most frequency ranges. Short- and long-term repeatability were generally comparable. Indices normalized to a subject's own baseline fared best, showing strong short- and long-term stability across all frequency intervals.

Conclusions
These results suggest that fine-resolution DPOAE-based measures of the MOC reflex measured at strategic frequencies are stable, and natural variance from day-to-day or week-to-week durations is small enough to detect between-group differences and possibly to monitor intervention-related success. However, this is an empirical question that must be directly tested to confirm its utility.