bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental study of fat grafting under negative pressure for wounds with exposed bone
Authors: Kao H-K , Hsu H-H , Chuang W-Y , Chang K-P , Chen B , Guo L 
Published: May 2015

Abstract

Background
The combination of fat grafting and negative pressure (VAC) therapy represents a synergistic interaction of all essential components for wound healing. The aim of this experimental study was to determine whether it could promote healing of wounds with exposed bone.

Methods
Full-thickness wounds with denuded bone in Sprague–Dawley rats were treated with either polyurethane foam dressing, fat grafting alone, polyurethane foam dressing with VAC, or polyurethane foam dressing with VAC combined with a single, or two administrations of fat graft. Wound healing kinetics, tissue growth, cell proliferation (Ki-67) and angiogenesis (platelet endothelial cell adhesion molecule 1 and α-smooth muscle actin) were investigated. Messenger RNA levels related to angiogenesis (vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (b-FGF)), profibrosis (platelet-derived growth factor A and transforming growth factor β), adipocyte expression (fatty acid-binding protein (FABP) 4 and peroxisome proliferator activated receptor γ), and extracellular matrix remodelling (collagen I) were measured in wound tissues.

Results
Wounds treated by VAC combined with fat grafting were characterized by cell proliferation, neoangiogenesis and maturation of functional blood vessels; they showed accelerated granulation tissue growth over the denuded bone compared with VAC- or foam dressing-treated wounds. Fat grafting alone over denuded bone resulted in complete necrosis. Expression of angiogenesis markers (VEGF and b-FGF) and adipocyte expression factors (FABP-4) was upregulated in wounds treated with VAC combined with fat grafting.

Conclusion
Fat grafting with VAC therapy may represent a simple but effective clinical solution for a number of complex tissue defects, and warrants testing in clinical models. Surgical relevanceThe combination of fat grafting and vacuum therapy represents a synergistic interaction of regenerative cells, hospitable wound matrix and stimulating micromechanical forces. It could accelerate complex wound healing through cell proliferation, neoangiogenesis and maturation of functional blood vessels. The efficacy of a multimodal wound healing approach is established in this experimental model; it could easily be translated into clinical trials of treatment for difficult wounds.
The Active Sites of a Rod‐Shaped Hollandite DeNOx Catalyst
Authors: Hu Pingping , Schuster Manfred Erwin , Huang Zhiwei , Xu Fei , Jin Shifeng , Chen Yaxin , Hua Weiming , Su Dang Sheng , Tang Xingfu 
Published: May 2015

AbstractThe identification of catalytically active sites (CASs) in heterogeneous catalysis is of vital importance to design and develop improved catalysts, but remains a great challenge. The CASs have been identified in the low‐temperature selective catalytic reduction of nitrogen oxides by ammonia (SCR) over a hollandite manganese oxide (HMO) catalyst with a rod‐shaped morphology and one‐dimensional tunnels. Electron microscopy and synchrotron X‐ray diffraction determine the surface and crystal structures of the one‐dimensional HMO rods closed by {100} side facets and {001} top facets. A combination of X‐ray absorption spectra, molecular probes with potassium and nitric oxide, and catalytic tests reveals that the CASs are located on the {100} side facets of the HMO rods rather than on the top facets or in the tunnels, and hence semi‐tunnel structural motifs on the {100} facets are evidenced to be the CASs of the SCR reaction. This work paves the way to further investigate the intrinsic mechanisms of SCR reactions.
Chirality Transfer and Modulation in LB Films Derived From the Diacetylene/Melamine Hydrogen‐Bonded Complex
Authors: Zhu Yu , Xu Yangyang , Zou Gang , Zhang Qijin 
Published: May 2015

AbstractIntroduction of hydrogen‐bonding interaction into π‐conjugated systems is a promising strategy, since the highly selective and directional hydrogen‐bonding can increase the binding strength, provide enhanced stability to the assemblies, and position the π‐conjugated molecules in a desired arrangement. The helical packing of the rigid melamine cores seems to play a dominating role in the subsequent formation of the peripheral helical PDA backbone. The polymerized Langmuir–Blodgett (LB) films exhibited reversible colorimetric and chiroptical changes during repeated heating–cooling cycles, which should be ascribed to the strong hydrogen‐bonding interaction between the carboxylic acid and the melamine core. Further, the closely helical packing of the melamine cores could be destroyed upon exposure to HCl or NH3 gas, whereas the peripheral helical polyaniline and polydiacetylene (PDA) backbone exhibited excellent stability. Although similar absorption changes could be observed for the films upon exposure to HCl or NH3 gas, their distinct circular dichroism (CD) responses enabled us to distinguish the above two stimuli. Chirality 27:492‐499, 2015. © 2015 Wiley Periodicals, Inc.
Novel Furin Inhibitors with Potent Anti‐infectious Activity
Authors: Hardes Kornelia , Becker Gero L. , Lu Yinghui , Dahms Sven O. , Köhler Susanne , Beyer Wolfgang , Sandvig Kirsten , Yamamoto Hiroyuki , Lindberg Iris , Walz Lisa , von Messling Veronika , Than Manuel E. , Garten Wolfgang , Steinmetzer Torsten 
Published: May 2015

AbstractNew peptidomimetic furin inhibitors with unnatural amino acid residues in the P3 position were synthesized. The most potent compound 4‐guanidinomethyl‐phenylacteyl‐Arg‐Tle‐Arg‐4‐amidinobenzylamide (MI‐1148) inhibits furin with a Ki value of 5.5 pM. The derivatives also strongly inhibit PC1/3, whereas PC2 is less affected. Selected inhibitors were tested in cell culture for antibacterial and antiviral activity against infectious agents known to be dependent on furin activity. A significant protective effect against anthrax and diphtheria toxin was observed in the presence of the furin inhibitors. Furthermore, the spread of the highly pathogenic H5N1 and H7N1 avian influenza viruses and propagation of canine distemper virus was strongly inhibited. Inhibitor MI‐1148 was crystallized in complex with human furin. Its N‐terminal guanidinomethyl group in the para position of the P5 phenyl ring occupies the same position as that found previously for a structurally related inhibitor containing this substitution in the meta position, thereby maintaining all of the important P5 interactions. Our results confirm that the inhibition of furin is a promising strategy for a short‐term treatment of acute infectious diseases.
Day Hospital Treatment for Anorexia Nervosa: A 12‐Month Follow‐up Study
Authors: Abbate‐Daga Giovanni , Marzola Enrica , De‐Bacco Carlotta , Buzzichelli Sara , Brustolin Annalisa , Campisi Stefania , Amianto Federico , Migliaretti Giuseppe , Fassino Secondo 
Published: May 2015

AbstractDay hospitals (DHs) represent a treatment option for anorexia nervosa (AN), a mental disorder that is difficult to treat and has no evidence‐based treatments available. We aimed to determine the effectiveness of a DH treatment that was specifically focused on the emotions of severe AN patients. Body mass index and eating psychopathology were the primary outcome measures.Fifty‐six adult patients with AN were assessed upon admission, at the end of treatment (EOT) and at a 12‐month follow‐up evaluation (T18) using Eating Disorders Inventory‐2, Beck Depression Inventory, Hamilton Rating Scale for Anxiety and Brief Social Phobia Scale. All participants received a multidisciplinary treatment programme that focused on psychodynamic psychotherapy.Seventy‐eight per cent of participants reported positive outcomes at EOT and 68% at T18. Moreover, 82.1% and 65.4% of long‐standing patients showed positive outcomes at EOT and T18, respectively. All measures of psychopathology were significantly improved at EOT and were maintained at follow‐up.Our DH was effective at treating severe AN patients; however, further investigations of the processes of change are warranted. Copyright © 2015 John Wiley & Sons, Ltd and Eating Disorders Association.
CO2 mineralization in volcanogenic sandstones: geochemical characterization of the Etchegoin formation, San Joaquin Basin
Authors: Zhang Shuo , DePaolo Donald J. , Voltolini Marco , Kneafsey Tim 
Published: May 2015

AbstractVolcanogenic sandstones are typically rich in volcanic rock fragments that can provide reactive minerals for CO2 mineralization in a scenario of CO2 sequestration. To quantitatively evaluate the extent and time scale of CO2 mineralization in potential reservoir formations, we characterized example high‐porosity volcanic sand from the San Joaquin Valley of southern Central California. Samples were collected from the volcanic‐rich members of the Etchegoin Formation in the San Joaquin Basin near Coalinga, California. Thin sections made from these samples were examined under petrographic microscope to identify mineral compositions, and then scanning electron microscopy (SEM) and energy dispersive x‐ray spectroscopy (EDX) were used to gather quantitative information on mineral abundances, distribution, and reactive surface areas. Porosity and permeability were also measured using core plugs made from outcrop samples. Results show that the Etchegoin volcanic sandstone has a high percentage (10–15%) of reactive minerals (pyroxenes, Fe‐Ti spinel and clays), and high reactive surface areas at about 1 m2/kg. Reactive transport modeling is conducted and shows that these reactive minerals could mineralize 92% of injected and capillary‐trapped CO2 within 1000 years of injection. Possible effects of heterogeneity on CO2 injection and mineralization are also studied using the reactive transport code TOUGHREACT. Vertical heterogeneity of mineralogy and hydrology increases both CO2 injectivity and mineralization. Available volcanogenic sandstones worldwide like the Etchegoin sandstone are summarized from the literature, with formations from the western USA that have CO2 sequestration potential emphasized.© 2015 Society of Chemical Industry and John Wiley & Sons, Ltd
Spatiotemporal variation and driving forces of reference evapotranspiration in Jing River Basin, northwest China
Authors: Xu Lihong , Shi Zhongjie , Wang Yanghui , Zhang Shulan , Chu Xinzheng , Yu Pengtao , Xiong Wei , Zuo Haijun , Wang Yunni 
Published: May 2015

AbstractEvapotranspiration is an important component of the hydrological cycle, which integrates atmospheric demands and surface conditions. Research on spatial and temporal variations of reference evapotranspiration (ETo) enables understanding of climate change and its effects on hydrological processes and water resources. In this study, ETo was estimated by the FAO‐56 Penman–Monteith method in the Jing River Basin in China, based on daily data from 37 meteorological stations from 1960 to 2005. ETo trends were detected by the Mann–Kendall test in annual, seasonal, and monthly timescales. Sensitivity coefficients were used to examine the contribution of important meteorological variables to ETo. The influence of agricultural activities, especially irrigation on ETo was also analyzed. We found that ETo showed a decreasing trend in most of the basin in all seasons, except for autumn, which showed an increasing trend. Mean maximum temperature was generally the most sensitive parameter for ETo, followed by relative humidity, solar radiation, mean minimum temperature, and wind speed. Wind speed was the most dominant factor for the declining trend in ETo. The more significant decrease in ETo for agricultural and irrigation stations was mainly because of the more significant decrease in wind speed and sunshine hours, a mitigation in climate warming, and more significant increase in relative humidity compared with natural stations and non‐irrigation stations. Changes in ETo and the sensitivity coefficient of meteorological variables in relation to ETo were also affected by topography. Better understanding of ETo response to climate change will enable efficient use of agricultural production and water resources, which could improve the ecological environment in Jing River Basin. Copyright © 2015 John Wiley & Sons, Ltd.
Effect of ultrasound treatment on the wet heating Maillard reaction between mung bean [Vigna radiate (L.)] protein isolates and glucose and on structural and physico‐chemical properties of conjugates
Authors: Wang Zhongjiang , Han Feifei , Sui Xiaonan , Qi Baokun , Yang Yong , Zhang Hui , Wang Rui , Li Yang , Jiang Lianzhou 
Published: May 2015

AbstractBACKGROUNDThe objective of this study was to determine the effect of ultrasound treatment on the wet heating Maillard reaction between mung bean protein isolates (MBPIs) and glucose, and on structural and physico‐chemical properties of the conjugates.RESULTSThe degree of glycosylation of MBPI–glucose conjugates treated by ultrasound treatment and wet heating (MBPI‐GUH) was higher than that of MBPI–glucose conjugates only treated by wet heating (MBPI‐GH). Solubility, emulsification activity, emulsification stability and surface hydrophobicity of MBPI‐GUH were higher than that of MBPI‐GH. Grafted MBPIs had a lower content of α‐helix and unordered coil, but a higher content of β‐sheet and β‐turn structure than MBPIs. No significant structural changes were observed in β‐turn and random coil structure of MBPI‐GUH, while α‐helix content increased with ultrasonic time, and decreased at 300 W ultrasonic power with the increase of β‐sheet. MBPI‐GUH had a less compact tertiary structure compared to MBPI‐GH and MBPI. Grafting MBPIs with glucose formed conjugates of higher molecular weight, while no significant changes were observed in electrophoresis profiles of MBPI‐GUH.CONCLUSIONUltrasound‐assisted wet heating Maillard reaction between MBPIs and glucose could be a promising way to improve functional properties of MBPIs. © 2015 Society of Chemical Industry
Kinetic studies of the reaction between pesticides and hydroxyl radical generated by laser flash photolysis
Authors: Gozzi Fábio , Oliveira Silvio C , Dantas Renato F , Silva Volnir O , Quina Frank H , Machulek Amilcar 
Published: May 2015

AbstractBACKGROUNDDue to contamination of the environment by pesticides and their mishandling, there is the need for treatment of contaminated sites and correct disposal of materials containing them. Thus, studies with advanced oxidation processes are expanding and can determine the rate constant of the hydroxyl radical with organic compounds of great importance in environmental contamination. In this context, the use of laser flash photolysis has been shown to be viable for the determination of these constants.RESULTSThe reaction rate constants of different pesticides with HO• in degassed acetonitrile have been determined. They were 1.6 × 109 M−1 s−1, 0.6 × 109 M−1 s−1, 1.2 × 109 M−1 s−1, 2.4 × 109 M−1 s−1 and 2.2 × 109 M−1 s−1 for the pesticides carbaryl, propoxur, fenoxycarb, ethoxysulfuron and chlorimuron‐ethyl, respectively. These values are about an order of magnitude smaller than the diffusion controlled rate and correlate with the relative rates of disappearance of the pesticides in the photo‐Fenton reaction in water.CONCLUSIONThe correlation of the relative rate constants determined by laser flash photolysis with the relative rates of photo‐Fenton degradation of the pesticides is compelling evidence for the participation of the hydroxyl radical in the degradation of these pesticides in the latter system. © 2015 Society of Chemical Industry
Patterns and treatment of neck metastases in patients with salivary gland cancers
Authors: Yoo Shin‐Hyuk , Roh Jong‐Lyel , Kim Seon‐Ok , Cho Kyung‐Ja , Choi Seung‐Ho , Nam Soon Yuhl , Kim Sang Yoon 
Published: May 2015

BackgroundManagement of the neck in patients with salivary gland cancer is controversial. This study aimed to identify clinicopathologic characteristics associated with regional metastases and regional recurrence in patients with salivary gland carcinoma.MethodsOf 363 study patients, 51 underwent therapeutic neck dissection (TND) and 312 underwent either elective neck dissection (END, n = 110) or no neck dissection (NoND, n = 202). Univariate and multivariate analyses were performed to identify clinicopathologic characteristics associated with regional metastases and recurrence.ResultsPathologic neck metastases were identified in 17/110 (15.5%) END patients and neck recurrence was identified in 5/202 (2.5%) NoND patients. Histologic grade (P < 0.001), tumor site (P = 0.008), and lymphovascular invasion (P < 0.001) were independent risk factors for neck metastases. Nodal metastases were more common in high‐grade tumors (P < 0.001), at levels I–III in END patients and levels I–V in TND patients. Multivariate analyses identified histologic grade (P = 0.020), clinical N2 classification (P = 0.007), and extranodal extension (P = 0.005) as independent factors for regional recurrence after treatment.ConclusionsPatients with high‐grade cancers should receive END involving levels I–III, whereas patients with node‐positive salivary cancers should receive comprehensive TND involving all levels (I–V). J. Surg. Oncol. 2015 111:1000–1006. © 2015 Wiley Periodicals, Inc.
Prognostic factors for low rectal cancer patients undergoing intersphincteric resection after neoadjuvant chemoradiation
Authors: Lee Soo Young , Jo Jeong Seon , Kim Hun Jin , Kim Chang Hyun , Kim Young Jin , Kim Hyeong Rok 
Published: May 2015

ObjectivesThis study aimed to investigate prognostic factors for low rectal cancer patients undergoing intersphincteric resection (ISR) following neoadjuvant chemoradiation (CRT).MethodsWe retrospectively reviewed 163 primary rectal cancer patients without distant metastasis who underwent ISR following neoadjuvant CRT between January 2006 and December 2011. Prognostic factors for disease‐free survival (DFS) and local recurrence‐free survival (LRFS) were evaluated.ResultsThe median follow‐up period was 53 months (range, 0–82 months). Kaplan–Meier survival analysis indicated different 3‐year DFS (stage 0, 96.2%; I, 84.8%; II, 72.9%; III, 38.0%) and 3‐year LRFS (stage 0, 100.0%; I, 92.4%; II, 91.1%; III, 70.9%) according to pathologic TNM stages, particularly stage III. Multivariable Cox regression analysis suggested that DFS was associated with ypT (3/4 vs. 0/1/2) and ypN (1/2 vs. 0) stages, whereas LRFS with ypN stage (1/2 vs. 0), tumor size (≥3.5 cm vs. <3.5 cm), and tumor height from the anal verge (≤2 cm vs. >2 cm).ConclusionsISR following CRT could be feasible in stage‐I and II low rectal cancer patients, but might be related to poor oncologic outcomes in those with stage‐III disease. The indication for ISR or abdominoperineal resection should be carefully evaluated in cases of low rectal cancer with suspicious lymph node metastasis after CRT. J. Surg. Oncol. 2015 111:1054–1058. © 2015 Wiley Periodicals, Inc.
Versatile pulse sequence device to conserve hyperpolarization for NMR and MRI studies
Authors: Ghosh Rajat K. , Kuzma Nicholas N. , Kadlecek Stephen J. , Rizi Rahim R. 
Published: May 2015

PurposeLevitt and co‐workers have described the M2S pulse sequence which transfers between longitudinal and singlet spin order. Building on this work, we describe the construction of a portable M2S pulse sequence generator to increase the relaxation time of polarized compounds. Additionally, we investigate the efficiency of spin order transfer under conditions where physical parameters of the system are not known precisely.Theory and MethodsA portable M2S generator is built. Longitudinally polarized N2O is converted to the singlet state by both adiabatic transfer and by the M2S sequence. Density matrix simulations are used to model the effects of mismatched chemical shift, flip angle, and scalar couplings.ResultsDensity matrix simulations suggest that to convert 95% of the longitudinal m = 1 triplet state population to the singlet order we must match the Larmor precession frequency to the excitation radiofrequency field by 10%, the scalar couplings must be determined to better than 0.6%, and the flip angle must be calibrated to better than 2%.ConclusionThe sequence is robust against many mismatched physical parameters of the species we are converting. Additionally, the instrument's portability allows for the conversion of hyperpolarized species near a polarizer. The lifetime is increased by ∼12‐fold. This is highly advantageous in systems where the hyperpolarized media relax rapidly. Magn Reson Med 75:1822–1830, 2016. © 2015 Wiley Periodicals, Inc.
Utilizing magnetization transfer imaging to investigate tissue remodeling in a murine model of autosomal dominant polycystic kidney disease
Authors: Kline Timothy L. , Irazabal Maria V. , Ebrahimi Behzad , Hopp Katharina , Udoji Kelly N. , Warner Joshua D. , Korfiatis Panagiotis , Mishra Prasanna K. , Macura Slobodan I. , Venkatesh Sudhakar K. , Lerman Lilach O. , Harris Peter C. , Torres Vicente E. , King Bernard F. , Erickson Bradley J. 
Published: May 2015

PurposeNoninvasive imaging techniques that quantify renal tissue composition are needed to more accurately ascertain prognosis and monitor disease progression in polycystic kidney disease (PKD). Given the success of magnetization transfer (MT) imaging to characterize various tissue remodeling pathologies, it was tested on a murine model of autosomal dominant PKD.MethodsC57Bl/6 Pkd1 R3277C mice at 9, 12, and 15 months were imaged with a 16.4T MR imaging system. Images were acquired without and with RF saturation in order to calculate MT ratio (MTR) maps. Following imaging, the mice were euthanized and kidney sections were analyzed for cystic and fibrotic indices, which were compared with statistical parameters of the MTR maps.ResultsThe MTR‐derived mean, median, 25th percentile, skewness, and kurtosis were all closely related to indices of renal pathology, including kidney weight/body weight, cystic index, and percent of remaining parenchyma. The correlation between MTR and histology‐derived cystic and fibrotic changes was R2 = 0.84 and R2 = 0.70, respectively.ConclusionMT imaging provides a new, noninvasive means of measuring tissue remodeling PKD changes and may be better suited for characterizing renal impairment compared with conventional MR techniques. Magn Reson Med 75:1466–1473, 2016. © 2015 The Authors. Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance.
Wideband arrhythmia‐Insensitive‐rapid (AIR) pulse sequence for cardiac T1 mapping without image artifacts induced by an implantable‐cardioverter‐defibrillator
Authors: Hong KyungPyo , Jeong Eun‐Kee , Wall T. Scott , Drakos Stavros G. , Kim Daniel 
Published: May 2015

PurposeTo develop and evaluate a wideband arrhythmia‐insensitive‐rapid (AIR) pulse sequence for cardiac T1 mapping without image artifacts induced by implantable‐cardioverter‐defibrillator (ICD).MethodsWe developed a wideband AIR pulse sequence by incorporating a saturation pulse with wide frequency bandwidth (8.9 kHz) to achieve uniform T1 weighting in the heart with ICD. We tested the performance of original and “wideband” AIR cardiac T1 mapping pulse sequences in phantom and human experiments at 1.5 Tesla.ResultsIn five phantoms representing native myocardium and blood and postcontrast blood/tissue T1 values, compared with the control T1 values measured with an inversion‐recovery pulse sequence without ICD, T1 values measured with original AIR with ICD were considerably lower (absolute percent error > 29%), whereas T1 values measured with wideband AIR with ICD were similar (absolute percent error < 5%). Similarly, in 11 human subjects, compared with the control T1 values measured with original AIR without ICD, T1 measured with original AIR with ICD was significantly lower (absolute percent error > 10.1%), whereas T1 measured with wideband AIR with ICD was similar (absolute percent error < 2.0%).ConclusionThis study demonstrates the feasibility of a wideband pulse sequence for cardiac T1 mapping without significant image artifacts induced by ICD. Magn Reson Med 74:336–345, 2015. © 2015 Wiley Periodicals, Inc.