bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term effects of maternal separation on the responsiveness of the circadian system to melatonin in the diurnal nonhuman primate (Macaca mulatta)
Authors: Rawashdeh Oliver , Dubocovich Margarita L. 
Published: January 2014

AbstractDepression is often linked to early‐life adversity and circadian disturbances. Here, we assessed the long‐term impact of early‐life adversity, particularly preweaning mother–infant separation, on the circadian system's responsiveness to a time giver or synchronizer (Zeitgeber). Mother‐reared (MR) and peer‐reared (PR) rhesus monkeys were subjected to chronic jet‐lag, a forced desynchrony protocol of 22 hr T‐cycles [11:11 hr light:dark (LD) cycles] to destabilize the central circadian organization. MR and PR monkeys subjected to the T‐cycles showed split locomotor activity rhythms with periods of ~22 hr (entrained) and ~24 hr (free‐running), simultaneously. Continuous melatonin treatment in the drinking water (20 μg/mL) gradually increased the amplitude of the entrained rhythm at the expense of the free‐running rhythm, reaching complete entrainment by 1 wk. Upon release into constant dim light, a rearing effect on anticipation for both the predicted light onset and food presentation was observed. In MR monkeys, melatonin did not affect the amplitude of anticipatory behavior. Interestingly, however, PR macaques showed light onset and food anticipatory activities in response to melatonin treatment. These results demonstrate for the first time a rearing‐dependent effect of maternal separation in macaques, imprinting long‐term plastic changes on the circadian system well into late adulthood. These effects could be counteracted by the synchronizer molecule melatonin. We conclude that the melatonergic system is targeted by early‐life adversity of maternal separation and that melatonin supplementation ameliorates the negative impact of stress on the circadian system.
Gender-Specificity of Solitary and Dyadic Sexual Desire among Gynephilic and Androphilic Women and Men
Authors: Dawson Samantha J. , Chivers Meredith L. 
Published: January 2014

Abstract

Introduction
Incentive motivation theory proposes that sexual desire emerges from sexual arousal, and is triggered by sexually competent stimuli. Research demonstrates gender and sexual orientation differences in the features that contribute to the competency of sexual stimuli. Men's and gynephilic women's genital arousal tends to be gender-specific with preferred gender eliciting significantly greater genital arousal than nonpreferred gender. In contrast, stimuli depicting preferred and nonpreferred gender elicit similar degrees of genital arousal among androphilic women, termed a gender-nonspecific pattern. Given these differences in the features that elicit a sexual response, and that sexual desire is proposed to emerge from sexual arousal, the question remains as to whether sexual desire would emerge only through exposure to preferred stimuli or whether patterns of responsive desire would parallel those observed for genital arousal.

Aim
The study aims to examine patterns of dyadic and solitary sexual desire in response to stimuli differing in incentive value.

Methods
Thirty androphilic women, 21 gynephilic women, 21 gynephilic men, and 16 androphilic men participated in a sexual psychophysiological session. Participants viewed sexual stimuli that varied the gender of the actors and the intensity of sexual activities depicted.

Main Outcome Measures
Participants reported their degree of desire for sex with a partner (dyadic desire) and desire to masturbate (solitary desire), before and after each film.

Results
Men and gynephilic women exhibited gender-specific patterns of sexual desire. Androphilic women's dyadic desire showed significantly less differentiation between genders, and their solitary desire did not differentiate at all. No gender difference was observed for either type of desire. All groups reported greater desire as stimulus intensity increased.

Conclusions
Gender-nonspecific sexual response is not limited to the sexual arousal patterns of androphilic women, but extends to include responsive sexual desire. Men and gynephilic women, however, show gender-specific responsive sexual desire that parallels their sexual arousal patterns.
Loss of intrahepatic HBsAg expression predicts sustained response to peginterferon and is reflected by pronounced serum HBsAg decline
Authors: Arends P. , Rijckborst V. , Zondervan P. E. , Buster E. , Cakaloglu Y. , Ferenci P. , Tabak F. , Akarca U. S. , Simon K. , Sonneveld M. J. , Hansen B. E. , Janssen H. L. A. 
Published: January 2014

SummaryThere is a lack of knowledge regarding the effect of peginterferon (PEG‐IFN) on the expression of intrahepatic hepatitis B core and surface antigen (HBcAg and HBsAg) in chronic hepatitis B (CHB) and its relation with response to therapy. Fifty‐two HBeAg‐positive and 67 HBeAg‐negative CHB patients with paired liver biopsies taken at baseline and after 1 year of PEG‐IFN therapy were studied. After PEG‐IFN therapy, HBeAg‐negative patients showed a significant reduction in both intrahepatic HBcAg (P = 0.04) and HBsAg expression (P < 0.001). In contrast, a reduction in intrahepatic HBcAg expression was not observed in HBeAg‐positive patients, while a trend in reduction of intrahepatic HBsAg staining was found (P = 0.09). Post‐treatment, 7 (13%) HBeAg‐positive and 9 (14%) HBeAg‐negative patients had no expression of intrahepatic HBsAg. Patients without any intrahepatic HBsAg expression post‐treatment were more likely to achieve a combined response (HBeAg loss with hepatitis B virus (HBV) DNA <2000 IU/mL for HBeAg ‐positive and HBV DNA <2000 IU/mL and normal alanine aminotransferase for HBeAg‐negative CHB): 71% vs 5% for HBeAg‐positive (P < 0.001) and 60% vs 16% for HBeAg‐negative patients (P = 0.004), respectively. Moreover, a more profound decline of serum HBsAg was observed in patients with absence of intrahepatic HBsAg staining (3.1 vs 0.4 log IU/mL, P < 0.001 and 1.7 vs 0.4 log IU/mL, P = 0.005 for HBeAg‐positive and HBeAg‐negative CHB, respectively). In conclusion, PEG‐IFN reduces expression of intrahepatic HBsAg. Loss of HBsAg as assessed by immunohistochemistry from the liver predicts a sustained response and is reflected in a pronounced serum HBsAg decline.
Building the bacterial orisome: high‐affinity DnaA recognition plays a role in setting the conformation of oriC DNA
Authors: Kaur Gulpreet , Vora Mansi P. , Czerwonka Christopher A. , Rozgaja Tania A. , Grimwade Julia E. , Leonard Alan C. 
Published: January 2014

SummaryDuring assembly of the E. coli pre‐replicative complex (pre‐RC), initiator DnaA oligomers are nucleated from three widely separated high‐affinity DnaA recognition sites in oriC. Oligomer assembly is then guided by low‐affinity DnaA recognition sites, but is also regulated by a switch‐like conformational change in oriC mediated by sequential binding of two DNA bending proteins, Fis and IHF, serving as inhibitor and activator respectively. Although their recognition sites are separated by up to 90 bp, Fis represses IHF binding and weak DnaA interactions until accumulating DnaA displaces Fis from oriC. It remains unclear whether high‐affinity DnaA binding plays any role in Fis repression at a distance and it is also not known whether all high‐affinity DnaA recognition sites play an equivalent role in oligomer formation. To examine these issues, we developed origin‐selective recombineering methods to mutate E. coli chromosomal oriC. We found that, although oligomers were assembled in the absence of any individual high‐affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential. We propose a model in which interaction of DnaA molecules at high‐affinity sites regulates oriC DNA conformation.
A transcriptomics‐based kinetic model for ethylene biosynthesis in tomato (Solanum lycopersicum) fruit: development, validation and exploration of novel regulatory mechanisms
Authors: Van de Poel Bram , Bulens Inge , Hertog Maarten L. A. T. M. , Nicolai Bart M. , Geeraerd Annemie H. 
Published: January 2014

Summary

The gaseous plant hormone ethylene is involved in many physiological processes including climacteric fruit ripening, in which it is a key determinant of fruit quality. A detailed model that describes ethylene biochemistry dynamics is missing. Often, kinetic modeling is used to describe metabolic networks or signaling cascades, mostly ignoring the link with transcriptomic data.

We have constructed an elegant kinetic model that describes the transfer of genetic information into abundance and metabolic activity of proteins for the entire ethylene biosynthesis pathway during fruit development and ripening of tomato (Solanum lycopersicum).

Our model was calibrated against a vast amount of transcriptomic, proteomic and metabolic data and showed good descriptive qualities. Subsequently it was validated successfully against several ripening mutants previously described in the literature. The model was used as a predictive tool to evaluate novel and existing hypotheses regarding the regulation of ethylene biosynthesis.

This bottom‐up kinetic network model was used to indicate that a side‐branch of the ethylene pathway, the formation of the dead‐end product 1‐(malonylamino)‐1‐aminocyclopropane‐1‐carboxylic acid (MACC), might have a strong effect on eventual ethylene production. Furthermore, our in silico analyses indicated potential (post‐) translational regulation of the ethylene‐forming enzyme ACC oxidase.
Drought enhances folivory by shifting foliar metabolomes in Quercus ilex trees
Authors: Rivas‐Ubach Albert , Gargallo‐Garriga Albert , Sardans Jordi , Oravec Michal , Mateu‐Castell Laia , Pérez‐Trujillo Míriam , Parella Teodor , Ogaya Romà , Urban Otmar , Peñuelas Josep 
Published: January 2014

Summary

At the molecular level, folivory activity on plants has mainly been related to the foliar concentrations of nitrogen (N) and/or particular metabolites.

We studied the responses of different nutrients and the whole metabolome of Quercus ilex to seasonal changes and to moderate field experimental conditions of drought, and how this drought may affect folivory activity, using stoichiometric and metabolomic techniques.

Foliar potassium (K) concentrations increased in summer and consequently led to higher foliar K : phosphorus (P) and lower carbon (C) : K and N : K ratios. Foliar N : P ratios were not lowest in spring as expected by the growth rate hypothesis. Trees exposed to moderate drought presented higher concentrations of total sugars and phenolics and these trees also experienced more severe folivory attack.

The foliar increases in K, sugars and antioxidant concentrations in summer, the driest Mediterranean season, indicated enhanced osmoprotection under natural drought conditions. Trees under moderate drought also presented higher concentrations of sugars and phenolics; a plant response to avoid water loss. These shifts in metabolism produced an indirect relationship between increased drought and folivory activity.
Carbon and nitrogen gain during the growth of orchid seedlings in nature
Authors: Stöckel Marcus , Těšitelová Tamara , Jersáková Jana , Bidartondo Martin I. , Gebauer Gerhard 
Published: January 2014

Summary

For germination and establishment, orchids depend on carbon (C) and nutrients supplied by mycorrhizal fungi. As adults, the majority of orchids then appear to become autotrophic. To compare the proportional C and nitrogen (N) gain from fungi in mycoheterotrophic seedlings and in adults, here we examined in the field C and N stable isotope compositions in seedlings and adults of orchids associated with ectomycorrhizal and saprotrophic fungi.

Using a new highly sensitive approach, we measured the isotope compositions of seedlings and adults of four orchid species belonging to different functional groups: fully and partially mycoheterotrophic orchids associated with narrow or broad sets of ectomycorrhizal fungi, and two adult putatively autotrophic orchids associated exclusively with saprotrophic fungi.

Seedlings of orchids associated with ectomycorrhizal fungi were enriched in 13C and 15N similarly to fully mycoheterotrophic adults. Seedlings of saprotroph‐associated orchids were also enriched in 13C and 15N, but unexpectedly their enrichment was significantly lower, making them hardly distinguishable from their respective adult stages and neighbouring autotrophic plants.

We conclude that partial mycoheterotrophy among saprotroph‐associated orchids cannot be identified unequivocally based on C and N isotope compositions alone. Thus, partial mycoheterotrophy may be much more widely distributed among orchids than hitherto assumed.
Simultaneous profiling of seed‐associated bacteria and fungi reveals antagonistic interactions between microorganisms within a shared epiphytic microbiome on Triticum and Brassica seeds
Authors: Links Matthew G. , Demeke Tigst , Gräfenhan Tom , Hill Janet E. , Hemmingsen Sean M. , Dumonceaux Tim J. 
Published: January 2014

Summary

In order to address the hypothesis that seeds from ecologically and geographically diverse plants harbor characteristic epiphytic microbiota, we characterized the bacterial and fungal microbiota associated with Triticum and Brassica seed surfaces.

The total microbial complement was determined by amplification and sequencing of a fragment of chaperonin 60 (cpn60). Specific microorganisms were quantified by qPCR. Bacteria and fungi corresponding to operational taxonomic units (OTU) that were identified in the sequencing study were isolated and their interactions examined.

A total of 5477 OTU were observed from seed washes. Neither total epiphytic bacterial load nor community richness/evenness was significantly different between the seed types; 578 OTU were shared among all samples at a variety of abundances. Hierarchical clustering revealed that 203 were significantly different in abundance on Triticum seeds compared with Brassica. Microorganisms isolated from seeds showed 99–100% identity between the cpn60 sequences of the isolates and the OTU sequences from this shared microbiome. Bacterial strains identified as Pantoea agglomerans had antagonistic properties toward one of the fungal isolates (Alternaria sp.), providing a possible explanation for their reciprocal abundances on both Triticum and Brassica seeds.

cpn60 enabled the simultaneous profiling of bacterial and fungal microbiota and revealed a core seed‐associated microbiota shared between diverse plant genera.
A eucrustacean from the Cambrian ‘Orsten’ of Sweden with epipods and a maxillary excretory opening
Authors: Waloszek Dieter , Maas Andreas , Olesen Jørgen , Haug Carolin , Haug Joachim T. 
Published: January 2014

AbstractThe Cambrian species Paulinecaris siveterae n. gen. n. sp., known from two trunk fragments, represents the first record of epipods (serving as gills and osmoregulatory structures) in a crustacean from the Swedish ‘Orsten’. Moreover, it is the first report of the maxillary excretory opening of a crustacean based on Cambrian material of ‘Orsten’‐type preservation. One specimen comprises the maxillary segment with an appendage and several thoracic segments with parts of their limbs; a second specimen is a fragment possibly of a more posterior part of the trunk. As in other known small eucrustaceans, the tergites of the new species lack prominent tergopleurae, so that the limbs insert directly ventral to the tergal margins. Limb preservation includes the maxilla and several thoracopods, all possessing a prominent, fleshy basipod with six setose endites along their median rim distally to the proximal endite. The presence of long and prominent limbs of P. siveterae suggests that it had good swimming ability, while the slight C‐like curvature of their basal limb part, basipod, indicates involvement of the limbs also in so‐called ‘sucking chambers’ for suspension feeding coupled with locomotion. The estimated total length of P. siveterae, 2–3 mm, is comparable to that of extant cephalocarids, but its appendages are twice as long and wide. The limbs of P. siveterae also differ in size and armature from extant eucrustaceans as well as early representatives of this group known from the ‘Orsten’ assemblages. The general morphology of the limbs, for example in having a fleshy and C‐shaped basipod with several setae‐bearing endites medially, identifies P. siveterae as an entomostracan eucrustacean, but a lack of further details precludes its affinity with any of the in‐group taxa. Three epipods on the outer edge of the basipod, as in P. siveterae, are also known from the Cambrian eucrustacean Yicaris dianensis from China and early ontogenetic stages of extant fairy shrimps (Anostraca); their adult stages have two epipods. This hints at an original number of three epipods in the ground pattern of Entomostraca, but some uncertainty remains with regard to the eucrustacean ground pattern because Malacostraca possess a maximum number of two.
The Effect of Loading Palladium on Zinc Oxide on the Photocatalytic Degradation of Methyl tert‐Butyl Ether (MTBE) in Water
Authors: Seddigi Zaki S. , Ahmed Saleh A. , Ansari Shahid P. , Yarkandi Naeema H. , Danish Ekram , Alkibash Abdullah Abu , Oteef Mohammed D. Y. , Ahmed Shakeel 
Published: January 2014

AbstractA series of heterogeneous catalysts was prepared by doping zinc oxide with different palladium loadings in the range of 0.5%–1.5%. The prepared catalysts were characterized by SEM, TEM and XRD. These catalysts were applied to study the degradation of Methyl tert‐Butyl Ether (MTBE). An amount of 100 mg of each of these catalysts was added to an aqueous solution of 100 ppm of MTBE. The resulting mixtures were irradiated with UV light for a period of 5 h. A 99.7% removal of MTBE was achieved in the case of the zinc oxide photocatalyst particles doped with 1% Pd. The photoreaction was found to be a first‐order one.
Gold Mineralization of the Gatsuurt Deposit in the North Khentei Gold Belt, Central Northern Mongolia
Authors: Khishgee Chinbat , Akasaka Masahide , Ohira Hiroto , Sereenen Jargalan 
Published: January 2014

AbstractMineral assemblages, chemical compositions of ore minerals, wall rock alteration and fluid inclusions of the Gatsuurt gold deposit in the North Khentei gold belt of Mongolia were investigated to characterize the gold mineralization, and to clarify the genetic processes of the ore minerals. The gold mineralization of the deposit occurs in separate Central and Main zones, and is characterized by three ore types: (i) low‐grade disseminated and stockwork ores; (ii) moderate‐grade quartz vein ores; and (iii) high‐grade silicified ores, with average Au contents of approximately 1, 3 and 5 g t−1 Au, respectively. The Au‐rich quartz vein and silicified ore mineralization is surrounded by, or is included within, the disseminated and stockwork Au‐mineralization region. The main ore minerals are pyrite (pyrite‐I and pyrite‐II) and arsenopyrite (arsenopyrite‐I and arsenopyrite‐II). Moderate amounts of galena, tetrahedrite‐tennantite, sphalerite and chalcopyrite, and minor jamesonite, bournonite, boulangerite, geocronite, scheelite, geerite, native gold and zircon are associated. Abundances and grain sizes of the ore minerals are variable in ores with different host rocks. Small grains of native gold occur as fillings or at grain boundaries of pyrite, arsenopyrite, sphalerite, galena and tetrahedrite in the disseminated and stockwork ores and silicified ores, whereas visible native gold of variable size occurs in the quartz vein ores. The ore mineralization is associated with sericitic and siliceous alteration. The disseminated and stockwork mineralization is composed of four distinct stages characterized by crystallization of (i) pyrite‐I + arsenopyrite‐I, (ii) pyrite‐II + arsenopyrite‐II, (iii) galena + tetrahedrite + sphalerite + chalcopyrite + jamesonite + bournonite + scheelite, and iv) boulangerite + native gold, respectively. In the quartz vein ores, four crystallization stages are also recognized: (i) pyrite‐I, (ii) pyrite‐II + arsenopyrite + galena + Ag‐rich tetrahedrite‐tennantite + sphalerite + chalcopyrite + bournonite, (iii) geocronite + geerite + native gold, and (iv) native gold. Two mineralization stages in the silicified ores are characterized by (i) pyrite + arsenopyrite + tetrahedrite + chalcopyrite, and (ii) galena + sphalerite + native gold. Quartz in the disseminated and stockwork ores of the Main zone contains CO2‐rich, halite‐bearing aqueous fluid inclusions with homogenization temperatures ranging from 194 to 327°C, whereas quartz in the disseminated and stockwork ores of the Central zone contains CO2‐rich and aqueous fluid inclusions with homogenization temperatures ranging from 254 to 355°C. The textures of the ores, the mineral assemblages present, the mineralization sequences and the fluid inclusion data are consistent with orogenic classification for the Gatsuurt deposit.
Geochemical and statistical characterization of the phosphate facies of the Farim‐Saliquinhé phosphate mineralization (Guinea‐Bissau)
Authors: Charifo Gilberto , Ferreira António M. , Almeida José A. , Prian Jean‐Pierre 
Published: January 2014

AbstractThe Eocene Farim‐Saliquinhé phosphate mineralization (FSPM) is a buried sedimentary deposit located in the northern part of Guinea‐Bissau, discovered and spatially constrained during the 1980s by the French Geological Survey (BRGM). In the present work some of the data collected at that time are reworked in the framework of the development of a 3D geological and geochemical model of this mineralization. We show the usefulness of two classical multivariate statistical methods – principal component analysis (PCA) and cluster analysis (CA) – applied to eight geochemical variables (P2O5, SiO2, Al2O3, Fe2O3, CaO, MgO, F and CO2) measured in 247 samples from phosphate facies, for differentiation of samples taken from the different phosphate facies recognized in the FSPM, namely the main ore FPA (30 to 35% high grade phosphate), the calcareous low grade phosphate FPB (10 to 20% P2O5) (both Lutetian), and a mineralized Upper Eocene to Lower Oligocene cover (mainly including the FPO level and a silico‐aluminous phosphate bed), overlying the FPA, which can also be considered a third set of phosphate facies associated with the FSPM.
Regional Trade Agreements and the Pattern of Trade: A Networks Approach
Authors: Reyes Javier , Wooster Rossitza , Shirrell Stuart 
Published: January 2014

AbstractThis paper uses a complex network approach for the analysis trade effects from regional trade agreements on world trade flows. We use bilateral trade data to compute the network community structure for every year between 1970 and 2000. We compare this to null community structures that emerge from various models based on regional and geographical classifications, the implementation of RTA's and gravity models of trade. Our results show that RTA formation appears to have a cyclical pattern on the world trade network community structure. We document periods where bilateral trade flows and the structure of the world trade network are consistent with those predicted by formation of RTAs. These cycles occur in 1980–86 and 1990–96. At the same time, we document periods in which the pattern in the world trade network is not explained by RTA formation. Two periods, 1986–90 and 1997–2000, show a pattern of bilateral trade flows that moves away from the prediction that results from assuming RTA formation as the driving force in the determination of the world trade network structure. Factors contributing to the latter parts of the cycle during our sample period include formation of regional trade networks in East Asia that account for a significant portion of world trade but are not formalised by RTAs in force.