bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analyses of tropistic responses using metabolomics
Authors: Millar Katherine D. L. , Kiss John Z. 
Published: November 2012

• Premise of the study: Characterization of phototropism and gravitropism has been through gene expression studies, assessment of curvature response, and protein expression experiments. To our knowledge, the current study is the first to determine how the metabolome, the complete set of small‐molecule metabolites within a plant, is impacted during these tropisms.• Methods: We have determined the metabolic profile of plants during gravitropism and phototropism. Seedlings of Arabidopsis thaliana wild type (WT) and phyB mutant were exposed to unidirectional light (red or blue) or reoriented to induce a tropistic response, and small‐molecule metabolites were assayed and quantified. A subset of the WT was analyzed using microarray experiments to obtain gene profiling data.• Key results: Analyses of the metabolomic data using principal component analysis showed a common profile in the WT during the different tropistic curvatures, but phyB mutants produced a distinctive profile for each tropism. Interestingly, the gravity treatment elicited the greatest changes in gene expression of the WT, followed by blue light, then by red light treatments. For all tropisms, we identified genes that were downregulated by a large magnitude in carbohydrate metabolism and secondary metabolism. These included ATCSLA15, CELLULOSE SYNTHASE‐LIKE, and ATCHS/SHS/TT4, CHALCONE SYNTHASE. In addition, genes involved in amino acid biosynthesis were strongly upregulated, and these included THA1 (THREONINE ALDOLASE 1) and ASN1 (DARK INDUCIBLE asparagine synthase).• Conclusions: We have established the first metabolic profile of tropisms in conjunction with transcriptomic analyses. This approach has been useful in characterizing the similarities and differences in the molecular mechanisms involved with phototropism and gravitropism.
Le traitement des phases paranoïdes dans les groupes de thérapie psychocorporelle en institution
Authors: Gaudriault Pierre , Pistre Florence 
Published: November 2012

Les groupes de thérapie psychocorporelle en institution soumettent les patients qui y participent à une triple régression. La première tient à l’abord corporel, la seconde est classiquement associée à la situation de groupe, la troisième est liée à la psychopathologie des patients admis dans ce genre de groupe. Ces régressions intenses peuvent avoir comme conséquence l’émergence d’angoisses paranoïdes massives. Après avoir décrit le dispositif tripartite utilisé (phase active et relationnelle, phase allongée et phase de verbalisation), nous allons centrer notre réflexion sur ces angoisses paranoïdes. Celles-ci sont réactivées par la triple régression et constituent un risque d’échec du traitement pour les patients qui n’entrent pas dans le cadre proposé. Le traitement de ces angoisses s’effectue pour les « survivants » en fonction des paramètres suivants : l’analyse du contre-transfert des thérapeutes, la solidité du groupe et l’accès au corps imaginaire subjectif chez les participants. À travers diverses vignettes cliniques, nous allons montrer que l’émergence de ces angoisses serait inhérente à ces dispositifs psychocorporels. Elles peuvent être à l’origine de crises difficiles mais salutaires quand elles sont surmontées.
Ontology: A Support Structure for a V-Labs Network: Euronet-Lab
Authors: Correia Raul Cordeiro 
Published: November 2012

Our propose is to build a network of virtual laboratories, based in a Virtual Closet that will contain all the elements and parts that are needed to build the various experiences available in a v-labs network (that we call Euronet-Lab).
To build this complex network we need to find a system that supports effectively this structure. This probably will be a enormous database of v-labs and independent elements, where will be possible sometimes to â??recycleâ? some of the elements. This means â??re-useâ? the same element several times in many experiences. To do this is necessary to have a structure that allows us to have several instances of the same element.
Itâ??s important that in our structure and virtual environment we can create several â??imagesâ? of the same reality and this images can be used simultaneously in different circuits/experiments. This means that we can create several instances of the same element, to be used in different experiences and exercises.
Genomic DNA Isolation and Purification of Two Endemic Medicinal Plants (Pterocarpus Santalinus Linn.F & Pimpinella Tirupatiensis Bal&Subr) of Seshachalam Hills, Tirumala.
Authors: Vipranarayana S. , V.Prasad T.N.V.K. , Rajinikanth A. , Damodharam T. 
Published: November 2012

Ptercarpus santalinus is an important endemic medicinal plant with high medicinal values that interfere with DNA extraction procedures and qualitative, quantitative agaros gel electrophoresis. An effective and low-cost protocol for isolating genomic DNA from the roots of Pterocarpus santilinus and Pimpinella tirupatiensis was described in this paper. It involved a modified CTAB method with distilled water pretreating samples. The A260/A280 absorbance ratio of extracted DNA was found to be free from polysaccharide, poly-phenols and tannins contaminants ranged from 2.2 to 2.8 within the high level of purity.
Utility of the Patient Health Questionnaire‐9 to Assess Suicide Risk in Patients With Systemic Sclerosis
Authors: Razykov Ilya , Hudson Marie , Baron Murray , Thombs Brett D. ,   Canadian Scleroderma Research Group
Published: November 2012

AbstractObjectiveItem 9 of the Patient Health Questionnaire‐9 (PHQ‐9), which inquires about both passive thoughts of death and active ideas of self‐harm, has been used to assess suicide risk in arthritis. The objectives of this study were 1) to determine the proportion of systemic sclerosis (SSc; scleroderma) patients who responded “yes” to item 9 who endorsed active suicidal ideation in response to more direct questions during a structured clinical interview, and 2) to report whether the PHQ‐8, which does not include item 9 from the PHQ‐9, performs similarly to the PHQ‐9.MethodsPatients were recruited from the Canadian Scleroderma Research Group Registry. The PHQ‐9 and Composite International Diagnostic Interview (CIDI) depression module were administered during a phone interview. Scores on the PHQ‐8 were calculated by removing item 9 from the PHQ‐9. Item 9 responses were compared to suicidal ideation and intent in the last year based on the CIDI. Scores on the PHQ‐8 and PHQ‐9 were compared using Pearson's correlations.ResultsThere were 345 patients interviewed, of whom 31 (9.0%) endorsed item 9 of the PHQ‐9. Of those, based on the CIDI, 14 (45.2%) had passive thoughts of suicide or death. Only 1 patient (3.2%) had thoughts about suicide in some detail at any point in the last 12 months. The correlation between PHQ‐9 and PHQ‐8 scores was 0.998.ConclusionItem 9 appears to identify many patients who do not report active suicidal ideation. The PHQ‐8 may be a better option for assessment of depressive symptoms than the PHQ‐9 in patients with SSc.
Interstitial Lung Disease in Anti–Jo‐1 Patients With Antisynthetase Syndrome
Authors: Marie I. , Josse S. , Hatron P. Y. , Dominique S. , Hachulla E. , Janvresse A. , Cherin P. , Mouthon L. , Vittecoq O. , Menard J.‐F. , Jouen F. 
Published: November 2012

AbstractObjectiveTo assess the outcome of interstitial lung disease (ILD) in anti–Jo‐1 patients with antisynthetase syndrome, determine predictive variables of ILD deterioration in these patients, and compare features of anti–Jo‐1 patients with and without ILD.MethodsNinety‐one anti–Jo‐1 patients were identified by medical records search in 4 medical centers. All of these patients had undergone pulmonary function tests (PFTs) and high‐resolution computed tomography (HRCT) scans.ResultsSixty‐six patients (72.5%) had ILD. Patients could be divided into 3 groups according to their presenting lung manifestations: acute onset of lung disease (n = 12), progressive onset of lung signs (n = 35), and asymptomatic patients exhibiting abnormalities consistent with ILD on PFTs and HRCT scans (n = 19). Sixteen patients had resolution of ILD; 39 and 11 patients experienced improvement and deterioration of ILD, respectively. ILD led to decreased functional status, since 29.8% of patients exhibited a marked reduction of activities due to ILD and 13.6% had respiratory insufficiency requiring oxygen therapy; 5 of 6 patients died due to ILD complications. Predictive parameters of ILD deterioration were HRCT scan pattern of usual interstitial pneumonia, respiratory muscle involvement, and age ≥55 years. Furthermore, anti–Jo‐1 patients with ILD, compared with those without, more frequently exhibited mechanic's hands and lower creatine kinase levels.ConclusionOur findings confirm that ILD is a frequent complication in anti–Jo‐1 patients, resulting in high morbidity. We suggest that patients with predictive factors of ILD deterioration may require more aggressive therapy. Finally, anti–Jo‐1 patients with ILD, compared with those without, may exhibit a particular clinical phenotype.
Decline of Mean Initial Prednisone Dosage From 10.3 to 3.6 mg/day to Treat Rheumatoid Arthritis Between 1980 and 2004 in One Clinical Setting, With Long‐Term Effectiveness of Dosages Less Than 5 mg/day
Authors: Pincus Theodore , Sokka Tuulikki , Castrejón Isabel , Cutolo Maurizio 
Published: November 2012

AbstractObjectiveTo analyze prednisone treatment from 1980–2004 in 308 patients with rheumatoid arthritis (RA), including 75 monitored over 4–8 years and 73 monitored over >8 years, for initial dose, long‐term doses and effectiveness, and adverse events.MethodsA database of all patients of a single rheumatologist included medications and Multidimensional Health Assessment Questionnaire (MDHAQ) scores at each visit. Proportions of patients whose initial prednisone dosages were >5, 5, or <5 mg/day were computed in 5‐year periods: 1980–1984, 1985–1989, 1990–1994, 1995–1999, and 2000–2004. Mean changes in MDHAQ function, pain, and Routine Assessment of Patient Index Data 3 (RAPID3) scores were compared in patients treated with <5 versus ≥5 mg/day of prednisone; scores and adverse events were analyzed in quartiles by treatment duration of ≤1, 1.1–4, 4.1–8, and >8 years.ResultsIn the respective 5‐year periods, the mean initial prednisone dosages were 10.3, 6.5, 5.1, 4.1, and 3.6 mg/day, with >5 mg/day in 49%, 16%, 7%, 7%, and 3% of patients, 5 mg/day in 51%, 80%, 70%, 26%, and 10% of patients, and <5 mg/day in 0%, 4%, 23%, 67%, and 86% of patients. Most patients received early concomitant methotrexate after 1990, and prednisone <5 mg/day was maintained indefinitely. Patients treated with prednisone ≥5 mg/day had poorer clinical status as baseline and followup. MDHAQ scores improved similarly in patients treated with <5 or ≥5 mg/day. Primary adverse events were skin thinning and bruising. New hypertension, diabetes mellitus, and cataracts occurred in <10% of all patients, and <13% of those treated longer than 8 years.ConclusionThe data suggest that many patients with RA might be treated effectively with initial and long‐term prednisone <5 mg/day, although further research and observational data are needed to characterize more fully effectiveness and safety.
Development of Eco‐Friendly Cotton Fabric Reinforced Polypropylene Composites: Mechanical, Thermal, and Morphological Properties
Authors: Rukmini K. , Ramaraj B. , Shetty Suchetana K. , Taraiya Ajay , Bandyopadhyay Sumanda , Siddaramaiah  
Published: November 2012

ABSTRACTTo determine the possibility of using cotton fabric (CF) as reinforcement in the thermoplastic polymer matrix, woven CF‐reinforced polypropylene (PP) composites were fabricated by the compression molding of PP sheets with 20% and 30 wt% of CF. The fabrication process involved laying up multiple layers of woven CF between precut extruded sheets of PP and compression molding to achieve 3.5–4.0 mm CF‐reinforced PP composite laminate sheets. From the laminated sheets, test specimens were prepared and tested for tensile, flexural, and impact properties. It was found that the reinforcement of PP with 30% CF increased the tensile strength from 21.87 to 28.07 MPa, tensile modulus from 618 to 1867 MPa, flexural strength from 21.7 to 45.3 MPa, and flexural modulus from 813 to 1925 MPa. However, the tensile elongation at break decreased marginally from 26.72% to 20.99%. A multiaxial impact test indicates reduction in the total energy absorbed and the peak force of the fabric‐reinforced laminates in comparison with neat PP. Coefficient of thermal expansion results on CF‐reinforced PP laminate sheet indicate reduction in thermal expansion. Dynamic mechanical analysis of CF‐reinforced PP shows an increase in the storage modulus and loss modulus. Scanning electron microscopic images reveal fibers pull out and breakages due to lack of an interfacial interaction between the CF and the PP matrix. © 2012 Wiley Periodicals, Inc. Adv Polym Techn 2013, 32, 21327; View this article online at wileyonlinelibrary.com. DOI 10.1002/adv.21327
Valgus malalignment is a risk factor for lateral knee osteoarthritis incidence and progression: Findings from the multicenter osteoarthritis study and the osteoarthritis initiative
Authors: Felson David T. , Niu Jingbo , Gross K. Douglas , Englund Martin , Sharma Leena , Cooke T. Derek V. , Guermazi Ali , Roemer Frank W. , Segal Neil , Goggins Joyce M. , Lewis C. Elizabeth , Eaton Charles , Nevitt Michael C. 
Published: November 2012

AbstractObjectiveTo study the effect of valgus malalignment on knee osteoarthritis (OA) incidence and progression.MethodsWe measured the mechanical axis from long limb radiographs from the Multicenter Osteoarthritis Study (MOST) and the Osteoarthritis Initiative (OAI) to define limbs with valgus malalignment (mechanical axis of ≥1.1° valgus) and examined the effect of valgus alignment versus neutral alignment (neither varus nor valgus) on OA structural outcomes. Posteroanterior radiographs and knee magnetic resonance (MR) images were obtained at the time of the long limb radiograph and at followup examinations. Lateral progression was defined as an increase in joint space narrowing (on a semiquantitative scale) in knees with OA, and incidence was defined as new lateral narrowing in knees without radiographic OA. We defined lateral cartilage damage and progressive meniscal damage as increases in cartilage or meniscus scores at followup on the Whole‐Organ Magnetic Resonance Imaging Score scale (for the MOST) or the Boston Leeds Osteoarthritis Knee Score scale (for the OAI). We used logistic regression with adjustment for age, sex, body mass index, and Kellgren/Lawrence grade, as well as generalized estimating equations, to evaluate the effect of valgus alignment versus neutral alignment on disease outcomes. We calculated odds ratios (ORs) and 95% confidence intervals (95% CIs).ResultsWe studied 5,053 knees (881 valgus) of subjects in the MOST cohort and 5,953 knees (1,358 valgus) of subjects in the OAI cohort. In both studies, all strata of valgus malalignment, including 1.1° to 3° valgus, were associated with an increased risk of lateral disease progression. In knees without radiographic OA, valgus alignment >3° was associated with incidence (e.g., in the MOST, adjusted OR 2.5 [95% CI 1.0–5.9]). Valgus alignment >3° was also associated with cartilage damage on MR imaging in knees without OA (e.g., in the OAI, adjusted OR 5.9 [95% CI 1.1–30.3]).We found a strong relationship of valgus malalignment with progressive lateral meniscal damage.ConclusionValgus malalignment increases the risk of knee OA radiographic progression and incidence as well as the risk of lateral cartilage damage. It may cause these effects, in part, by increasing the risk of meniscal damage.
Am80, a retinoic acid receptor agonist, ameliorates murine vasculitis through the suppression of neutrophil migration and activation
Authors: Miyabe Chie , Miyabe Yoshishige , Miura Noriko N. , Takahashi Kei , Terashima Yuya , Toda Etsuko , Honda Fumiko , Morio Tomohiro , Yamagata Naoko , Ohno Naohito , Shudo Koichi , Suzuki Jun‐Ichi , Isobe Mitsuaki , Matsushima Kouji , Tsuboi Ryoji , Miyasaka Nobuyuki , Nanki Toshihiro 
Published: November 2012

AbstractObjectiveVasculitis is characterized by leukocyte infiltration in the vessel walls, with destructive damage to mural structures. Retinoids are compounds that bind to retinoic acid receptors and exert biologic activities similar to those of vitamin A, including modulatory effects on cell proliferation and differentiation. This study was undertaken to examine the therapeutic effects of a synthetic retinoid, Am80, in a murine model of vasculitis induced by Candida albicans water‐soluble fraction (CAWS).MethodsVasculitis was induced in BALB/c mice by intraperitoneal injection of CAWS. Neutrophils were depleted by injection of antineutrophil antibody–positive serum. Am80 was administered orally once daily. Vasculitis was evaluated histologically. Migration of labeled adoptively transferred cells was quantified. Chemotaxis was assessed by cell mobility analysis. Production of reactive oxygen species (ROS) and phosphorylation of MAPKs were measured by flow cytometry. Concentrations of elastase were measured by enzyme‐linked immunosorbent assay.ResultsAdministration of CAWS induced vasculitis in the coronary arteries and aortic root, with abundant neutrophil infiltration. Depletion of neutrophils reduced CAWS‐induced vasculitis. Treatment with Am80 led to a significant attenuation of the vasculitis score and inhibition of the migration of transferred neutrophils into the site of vasculitis. In vitro, Am80 suppressed fMLP‐induced chemotaxis of human peripheral blood neutrophils. ROS production and elastase release by stimulated neutrophils were reduced by AM80 treatment, and Am80 also inhibited phosphorylation of ERK‐1/2 and p38 in neutrophils stimulated with fMLP plus lipopolysaccharide.ConclusionAm80 significantly suppressed CAWS‐induced vasculitis. This effect was presumably exerted via inhibition of neutrophil migration and activation.
An In Situ Carbonization–Replication Method to Synthesize Mesostructured WO3/C Composite as Nonprecious‐Metal Anode Catalyst in PEMFC
Authors: Cui Xiangzhi , Hua Zile , Wei Chenyang , Shu Zhu , Zhang Liangxia , Chen Hangrong , Shi Jianlin 
Published: November 2012

AbstractA meostructured WO3/C composite with crystalline framework and high electric conductivity has been synthesized by a new in situ carbonization–replication route using the block copolymer (poly(ethylene glycol)‐block‐poly(propylene glycol)‐block‐poly(ethylene glycol)) present in situ in the pore channels of mesoporous silica template as carbon source. X‐ray diffraction, X‐ray photoelectron spectroscopy, transmission electron microscopy, thermogravimetry differential thermal analysis, and N2 adsorption techniques were adopted for the structural characterization. Cyclic voltammetry, chronoamperometry, and single‐cell test for hydrogen electrochemical oxidation were adopted to characterize the electrochemical activities of the mesoporous WO3/C composite. The carbon content and consequent electric conductivity of these high‐surface‐area (108–130 m2 g−1) mesostructured WO3/C composite materials can be tuned by variation of the duration of heat treatment, and the composites exhibited high and stable electrochemical catalytic activity. The single‐cell test results indicated that the mesostructured WO3/C composites showed clear electrochemical catalytic activity toward hydrogen oxidation at 25 °C, which makes them potential non‐precious‐metal anode catalysts in proton exchange membrane fuel cell.
Randomized clinical trial of self-gripping mesh versus sutured mesh for Lichtenstein hernia repair
Authors:   Danish Multicentre DANGRIP Study Group, Jorgensen L N , Sommer T , Assaadzadeh S , Strand L , Dorfelt A , Hensler M , Rosenberg J 
Published: November 2012

Abstract

Background
Many patients develop discomfort after open repair of a groin hernia. It was hypothesized that suture fixation of the mesh is a cause of these symptoms.

Methods
This patient- and assessor-blinded randomized multicentre clinical trial compared a self-gripping mesh (Parietene Progrip®) and sutured mesh for open primary repair of uncomplicated inguinal hernia by the Lichtenstein technique. Patients were assessed before surgery, on the day of operation, and at 1 and 12 months after surgery. The primary endpoint was moderate or severe symptoms after 12 months, including a combination of chronic pain, numbness and discomfort.

Results
The intention-to-treat population comprised 163 patients with self-gripping mesh and 171 with sutured mesh. The 12-month prevalence of moderate or severe symptoms was 17·4 and 20·2 per cent respectively (P = 0·573). There were no significant differences between the groups in postoperative complications (33·7 versus 40·4 per cent; P = 0·215), rate of recurrent hernia within 1 year (1·2 per cent in both groups) or quality of life.

Conclusion
The avoidance of suture fixation using a self-gripping mesh was not accompanied by a reduction in chronic symptoms after inguinal hernia repair. Registration number: NCT00815698 (http://www.clinicaltrials.gov).
Anatomic basis of radical cystectomy and orthotopic urinary diversion in female patients
Authors: Clifton Marisa M. , Tollefson Matthew K. 
Published: November 2012

AbstractLong considered oncologically hazardous or functionally damaging, radical cystectomy with orthotopic urinary diversion is the accepted standard of care for invasive bladder cancer. A number of anatomical and clinical observations have come together to make orthotopic urinary diversion possible for female patients. Not only have these observations led to the development of an oncologically safe, low‐pressure reservoir for urine, but also have improved the postoperative quality of life. Urethral sparing technique is safe for patients without bladder neck or trigonal tumors. Furthermore, improved understanding of the female urethral rhabdosphincter has decreased the likelihood of postoperative urinary incontinence. Finally, female sexual function may be preserved in patients who undergo preservation of the neurovascular tissue lateral to the vagina. These improvements have solidified orthotopic urinary diversion as the procedure of choice for selected female patients requiring radical cystectomy. Clin. Anat. 26:105–109, 2013. © 2012 Wiley Periodicals, Inc.
If I want you to like me, should I be like you or unlike you? The effect of prior positive interaction with the group on conformity and distinctiveness in consumer decision making
Authors: Papyrina Veronika 
Published: November 2012

ABSTRACTThe extant research points to conflicting results regarding social influence in consumer decision making. On the one hand, there is empirical evidence that suggests that people conform to other members of their groups. On the other hand, several studies demonstrated the opposite pattern, namely, that individuals seek distinctiveness from others in the group. The goal of the present research is to reconcile these contradictory findings. To this end, I propose that whether a person will conform to or seek distinctiveness from others in a particular consumption situation is contingent on the absence or presence of one's prior positive interaction with the group. I also suggest that this effect will occur in a public context, that is, when an individual's choice is visible to other group members. The results of experiment supported these propositions. Copyright © 2012 John Wiley & Sons, Ltd.