bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endogenous Myeloperoxidase Is a Mediator of Joint Inflammation and Damage in Experimental Arthritis
Authors: Odobasic Dragana , Yang Yuan , Muljadi Ruth C. M. , O'Sullivan Kim M. , Kao Wenping , Smith Malcolm , Morand Eric F. , Holdsworth Stephen R. 
Published: December 2013

ObjectiveMyeloperoxidase (MPO) is implicated as a local mediator of tissue damage when released extracellularly in many chronic inflammatory diseases. The purpose of this study was to explore the role of endogenous MPO in experimental rheumatoid arthritis (RA).MethodsK/BxN serum–transfer arthritis was induced in C57BL/6 wild‐type (WT) and MPO knockout (MPO−/−) mice, and disease development was assessed. MPO activity was measured in joint tissues from mice with or without K/BxN arthritis. Collagen‐induced arthritis (CIA) was induced in WT and MPO−/− mice, and disease development and immune responses were examined. MPO expression was assessed in synovial biopsy samples from patients with active RA, and the effect of MPO on synovial fibroblasts was tested in vitro.ResultsMPO was up‐regulated in the joints of mice with K/BxN arthritis, and MPO deficiency attenuated the severity of the disease without affecting circulating cytokine levels. In CIA, MPO−/− mice had enhanced CD4+ T cell responses and reduced frequency of regulatory T cells in the lymph nodes and spleen, as well as augmented interleukin‐17A and diminished interferon‐γ secretion by collagen‐stimulated splenocytes, without an effect on circulating anticollagen antibody levels. Despite enhanced adaptive immunity in secondary lymphoid organs, CIA development was attenuated in MPO−/− mice. Intracellular and extracellular MPO was detected in the synovium of patients with active RA, and human MPO enhanced the proliferation and decreased the apoptosis of synovial fibroblasts in vitro.ConclusionMPO contributes to the development of arthritis despite suppressing adaptive immunity in secondary lymphoid organs. This suggests distinct effects of local MPO on arthritogenic effector responses.
Assessment of Osteoarthritis Candidate Genes in a Meta‐Analysis of Nine Genome‐Wide Association Studies
Authors: Rodriguez‐Fontenla Cristina , Calaza Manuel , Evangelou Evangelos , Valdes Ana M. , Arden Nigel , Blanco Francisco J. , Carr Andrew , Chapman Kay , Deloukas Panos , Doherty Michael , Esko Tõnu , Garcés Aletá Carlos M. , Gomez‐Reino Carnota Juan J. , Helgadottir Hafdis , Hofman Albert , Jonsdottir Ingileif , Kerkhof Hanneke J. M. , Kloppenburg Margreet , McCaskie Andrew , Ntzani Evangelia E. , Ollier William E. R. , Oreiro Natividad , Panoutsopoulou Kalliope , Ralston Stuart H. , Ramos Yolande F. , Riancho Jose A. , Rivadeneira Fernando , Slagboom P. Eline , Styrkarsdottir Unnur , Thorsteinsdottir Unnur , Thorleifsson Gudmar , Tsezou Aspasia , Uitterlinden André G. , Wallis Gillian A. , Wilkinson J. Mark , Zhai Guangju , Zhu Yanyan ,   the arcOGEN Consortium, Felson David T. , Ioannidis John P. A. , Loughlin John , Metspalu Andres , Meulenbelt Ingrid , Stefansson Kari , van Meurs Joyce B. , Zeggini Eleftheria , Spector Timothy D. , Gonzalez Antonio 
Published: December 2013

ObjectiveTo assess candidate genes for association with osteoarthritis (OA) and identify promising genetic factors and, secondarily, to assess the candidate gene approach in OA.MethodsA total of 199 candidate genes for association with OA were identified using Human Genome Epidemiology (HuGE) Navigator. All of their single‐nucleotide polymorphisms (SNPs) with an allele frequency of >5% were assessed by fixed‐effects meta‐analysis of 9 genome‐wide association studies (GWAS) that included 5,636 patients with knee OA and 16,972 control subjects and 4,349 patients with hip OA and 17,836 control subjects of European ancestry. An additional 5,921 individuals were genotyped for significantly associated SNPs in the meta‐analysis. After correction for the number of independent tests, P values less than 1.58 × 10−5 were considered significant.ResultsSNPs at only 2 of the 199 candidate genes (COL11A1 and VEGF) were associated with OA in the meta‐analysis. Two SNPs in COL11A1 showed association with hip OA in the combined analysis: rs4907986 (P = 1.29 × 10−5, odds ratio [OR] 1.12, 95% confidence interval [95% CI] 1.06−1.17) and rs1241164 (P = 1.47 × 10−5, OR 0.82, 95% CI 0.74−0.89). The sex‐stratified analysis also showed association of COL11A1 SNP rs4908291 in women (P = 1.29 × 10−5, OR 0.87, 95% CI 0.82−0.92); this SNP showed linkage disequilibrium with rs4907986. A single SNP of VEGF, rs833058, showed association with hip OA in men (P = 1.35 × 10−5, OR 0.85, 95% CI 0.79−0.91). After additional samples were genotyped, association at one of the COL11A1 signals was reinforced, whereas association at VEGF was slightly weakened.ConclusionTwo candidate genes, COL11A1 and VEGF, were significantly associated with OA in this focused meta‐analysis. The remaining candidate genes were not associated.
Long Noncoding RNA Related to Cartilage Injury Promotes Chondrocyte Extracellular Matrix Degradation in Osteoarthritis
Authors: Liu Qiang , Zhang Xin , Dai Linghui , Hu Xiaoqing , Zhu Jingxian , Li La , Zhou Chunyan , Ao Yingfang 
Published: December 2013

ObjectiveLong noncoding RNAs (lncRNAs) play crucial regulatory roles in diverse biologic processes, but knowledge of lncRNAs in osteoarthritis (OA) is limited. The aim of this study was to identify lncRNA expression in articular cartilage and to explore the function of cartilage injury–related lncRNAs (lncRNA‐CIR) in OA.MethodsTo identify lncRNAs specifically expressed in OA cartilage, we compared the expression of lncRNAs in OA cartilage with that in normal cartilage using microarray and quantitative polymerase chain reaction (qPCR) analyses. In OA cartilage, lncRNA‐CIR was specifically, differentially, and highly expressed. The function of lncRNA‐CIR was determined by silencing and overexpression in vitro. Extracellular matrix (ECM)–related molecules were detected by qPCR, Western blot, and immunofluorescence analyses.ResultsUp to 152 lncRNAs were found to be differentially expressed (>8‐fold) in OA and normal cartilage (82 lncRNAs more highly expressed and 70 less highly expressed in OA cartilage than in normal cartilage). A specific differentially expressed lncRNA‐CIR was selected according to the results of the higher expression in OA cartilage and OA chondrocytes. The expression of lncRNA‐CIR increased in chondrocytes with in vitro treatment with interleukin‐1β and tumor necrosis factor α. Silencing of lncRNA‐CIR by small interfering RNA promoted the formation of collagen and aggrecan and reduced the expression of matrix‐degrading enzymes, such as MMP13 and ADAMTS5. The expression of collagen and aggrecan was reduced, whereas the expression of matrix‐degrading enzymes was increased, after overexpression of lncRNA‐CIR.ConclusionThe results indicate that lncRNA‐CIR contributes to ECM degradation and plays a key role in the pathogenesis of OA. We propose that lncRNA‐CIR could be used as a potential target in OA therapy.
Halofuginone Ameliorates Autoimmune Arthritis in Mice by Regulating the Balance Between Th17 and Treg Cells and Inhibiting Osteoclastogenesis
Authors: Park Mi‐Kyung , Park Jin‐Sil , Park Eun‐Mi , Lim Mi‐Ae , Kim Sung‐Min , Lee Dong‐Gun , Baek Seung‐Ye , Yang Eun‐Ji , Woo Jung‐Won , Lee Jennifer , Kwok Seung‐Ki , Kim Ho‐Youn , Cho Mi‐La , Park Sung‐Hwan 
Published: December 2013

ObjectiveThe small molecule halofuginone has been shown to inhibit fibrosis, angiogenesis, and tumor progression. This study was undertaken to evaluate the effects of halofuginone in preventing autoimmune arthritis in mice.MethodsThe effects of halofuginone on joint diseases were assessed by clinical scoring and histologic analysis. Protein expression levels were confirmed by immunohistochemistry, enzyme‐linked immunosorbent assay, flow cytometry, and/or Western blotting. The expression levels of messenger RNA (mRNA) for various molecules were determined by real‐time polymerase chain reaction (PCR). Proliferation of osteoclast precursors was assessed by bromodeoxyuridine uptake. Osteoclast differentiation and activity were determined by quantifying tartrate‐resistant acid phosphatase (TRAP)–positive multinucleated cells and area of resorbed bone.ResultsTreatment with halofuginone suppressed the development of autoimmune arthritis and reciprocally regulated Th17 cells and FoxP3+ Treg cells. These effects of halofuginone on Th17 differentiation involved increased signaling of ERK and reduction of STAT‐3 and NF‐ATc1 expression. Furthermore, halofuginone induced the expression of indoleamine 2,3‐dioxygenase (IDO) in dendritic cells, leading to reduced production of Th17 cells. In addition, halofuginone prevented the formation and activity of osteoclasts through suppression of transcription factors, such as activator protein 1 and NF‐ATc1, and inhibited cell cycle arrest by the committed osteoclast precursors via expression of Ccnd1 encoding cyclin D1.ConclusionTaken together, our results suggest that halofuginone is a promising therapeutic agent for the treatment of Th17 cell–mediated inflammatory diseases and bone diseases.
The Dynamic Feedback Matrix Control for Multidimensional Chaotic Systems
Authors: Zhang Fangfang , Liu Shutang 
Published: December 2013

AbstractMultidimensional chaotic systems are difficult to control because of their nonlinearity and coupling relationship among state variables. We present the error feedback matrix for nonlinear systems, which contains coupling information of state variables and updates online according to the gradient descent method. The error feedback matrix is similar to the state feedback matrix for linear systems, however, the former is time‐varying and converges to a constant matrix. Using the coupling relationship, we give a simple controller only by a few elements of the matrix and present whole matrix control and partial matrix control. Finally, we apply these schemes to a three‐dimensionalHenon system and make mathematical analysis and simulation experiments, which verify the effectiveness of the proposed controllers.
Two‐step transpeptidation of the insulin precursor expressed in Pichia pastoris to insulin ester via trypsin‐catalyzed cleavage and coupling
Authors: Liu Haifeng , Zhou Xiangshan , Tian Shousheng , Hao Xianghui , You Jinhua , Zhang Yuanxing 
Published: December 2013

AbstractInsulin precursor fusion protein expressed in Pichia pastoris is a single‐chain protein with a spacer peptide (EEAEAEAEPK) localized at its N‐terminal. Currently, the one‐step transpeptidation reaction with low yield and high cost is generally employed to convert the insulin precursor fusion protein into human insulin ester. In this study, a two‐step transpeptidation reaction was proposed separating the cleavage step from the coupling step so that each reaction was performed under its optimal conditions. Using this method, the total efficiency doubled and the reaction time was shortened compared with the one‐step method. In addition, the amount of O‐t‐butyl‐l‐threonine t‐butyl ester and trypsin dosages were reduced by 50% and 75%, respectively. This two‐step transpeptidation strategy was simple and efficient and could be used for the pharmaceutical production of human insulin.
Endogenous microRNA clusters outperform chimeric sequence clusters in Chinese hamster ovary cells
Authors: Klanert Gerald , Jadhav Vaibhav , Chanoumidou Konstantina , Grillari Johannes , Borth Nicole , Hackl Matthias 
Published: December 2013

AbstractMicroRNAs (miRNAs) are small non‐coding RNAs (∼22 nucleotides) which regulate gene expression by silencing mRNA translation. MiRNAs are transcribed as long primary transcripts, which are enzymatically processed by Drosha/Dgcr8, in the nucleus, and by Dicer in the cytoplasm, into mature miRNAs. The importance of miRNAs for coordinated gene expression is commonly accepted. Consequentially, there is a growing interest in the application of miRNAs to improve phenotypes of mammalian cell factories such as Chinese hamster ovary (CHO) cells. Few studies have reported the targeted over‐expression of miRNAs in CHO cells using vector‐based systems. These approaches were hampered by limited sequence availability, and required the design of “chimeric” miRNA genes, consisting of the mature CHO miRNA sequence encompassed by murine flanking and loop sequences. Here we show that the substitution of chimeric sequences with CHO‐specific sequences for expression of miRNA clusters yields significantly higher expression levels of the mature miRNA in the case of miR–221/222 and miR–15b/16. Our data suggest that the Drosha/Dgcr8‐mediated excision from primary transcripts is reduced for chimeric miRNA sequences compared to the endogenous sequence. Overall, this study provides important guidelines for the targeted over‐expression of clustered miRNAs in CHO cells.See accompanying commentary by Baik and Lee DOI: 10.1002/biot.201300503
Splenic vein thrombosis and pancreatic fistula after minimally invasive distal pancreatectomy
Authors: Kang C M , Chung Y E , Jung M J , Hwang H K , Choi S H , Lee W J 
Published: December 2013

Abstract

Background
This study aimed to investigate the clinical relevance of splenic vein thrombosis (SVT) in the splenic vein remnant following minimally invasive distal pancreatosplenectomy (DPS).

Methods
Medical records of patients who underwent laparoscopic or robotic distal pancreatectomy (DP) with or without splenectomy between January 2006 and August 2012 were reviewed. Rates of SVT and clinically relevant postoperative pancreatic fistula (POPF) were compared in a group of patients undergoing DPS and a group having spleen-preserving DP.

Results
Seventy-nine patients had minimally invasive DP, of whom 38 (48 per cent) developed SVT in the splenic vein remnant. DPS was associated with POPF (P = 0·001) and SVT (P &lt; 0·001). SVT length was closely related to the amount of peripancreatic fluid collection (P = 0·025) and POPF (P = 0·045). In a comparison of splenic vessel-sacrificing, spleen-preserving DP and DPS, postoperative platelet count was significantly higher in the DPS group (P &lt; 0·001). In addition, grade of SVT (P = 0·092) and POPF (P = 0·065) tended to be associated with DPS, suggesting that SVT may be related to both splenectomy and POPF.

Conclusion
Minimally invasive DPS is associated with SVT and POPF. Preservation of the spleen should be considered when treating patients with benign and borderline malignant tumours of the distal pancreas.
Patient exposure to X‐rays during coronary angiography and percutaneous transluminal coronary intervention: Results of a multicenter national survey
Authors: Georges Jean‐Louis , Belle Loic , Ricard Cécile , Cattan Simon , Albert Franck , Hirsch Jean‐Lou , Monsegu Jacques , Dibie Alain , Khalife Khalife , Caussin Christophe , Maccia Carlo , Livarek Bernard , Hanssen Michel ,   on behalf of the RAY'ACT investigators
Published: December 2013

ObjectivesTo evaluate practices for the protection from radiation of patients during coronary angiography (CA) and percutaneous coronary intervention (PCI), and to update reference values for the main radiation dose parameters.BackgroundFew multicenter data from large populations exist on radiation doses to patients during CA and PCI.MethodsRAY'ACT is a multicenter, nationwide French survey, with retrospective analysis of radiation parameters routinely registered in professional software from 33,937 CAs and 27,826 PCIs performed at 44 centers from January 1, through December 31, 2010.ResultsKerma‐area product (KAP) was registered in 91.7% (44/48) of centers and in 91.5% of procedures for CA (median, 27.2 Gy·cm2, interquartile range [IQR], 15.5–45.2) and 91.1% for PCI (median, 56.8 Gy·cm2, IQR, 32.8–94.6). Fluoroscopy time was registered in 87.5% (42/48) of centers and in 83.1% of procedures (median, 3.7 min, IQR, 2.3–6.3 for CA; 10.3 min, 6.7–16.2 for PCI). Variability across centers was high. Old equipment and routine left ventriculography were more common and number of registered frames and frame rate were higher in centers delivering high doses. The radial route was associated with lower doses than the femoral route (median KAP 26.8 Gy·cm2 [15.1–44.25] vs. 28.1 [16.4–46.9] for CA, respectively; and 55.6 Gy·cm2 [32.2–92.1] vs. 59.4 [24.6–99.9] for PCI, respectively; P < 0.01).ConclusionsThis survey showed a very high rate of compliance with dose registration during CA and PCI in French nonacademic hospitals. Updated diagnostic reference values are established for the main dose parameters (KAP, 45 Gy·cm2 for CA, 95 Gy·cm2 for PCI). © 2013 Wiley Periodicals, Inc.
Balloon aortic valvuloplasty in high risk aortic stenosis patients with left ventricular ejection fractions <20%
Authors: Pedersen Wes R. , Goldenberg Irvin F. , Pedersen Christopher W. , Lesser Andrew , Harris Kevin M. , Lesser John R. , Garberich Ross F. , Schwartz Jonathan G. , Shank Emily , Schwartz Robert S. 
Published: December 2013

ObjectivesTo determine outcomes following balloon aortic valvuloplasty (BAV) in aortic stenosis (AS) patients with a left ventricular ejection fraction (LVEF) <20%.BackgroundSevere AS patients with a LVEF <20% are excluded from United States (U.S.) transcatheter aortic valve replacement (TAVR) trials and often surgical aortic valve replacement (AVR). The role for BAV to enhance LVEF is unclear.MethodsOur BAV database of 270 consecutive patients extending from 2005 through 2010 was queried for a preoperative LVEF <20%. Demographics, echocardiograms, procedural technique, and outcomes were analyzed. Pre‐ and postoperative echocardiograms were used to determine improvement in aortic valve area (AVA) and LVEF.ResultsSixteen patients were identified with a median age of 82 years. The composite Society of Thoracic Surgeons' (STS) mortality risk was 16.4%. The median preoperative AVA and LVEF were 0.60 cm2 and 16%, respectively, and postoperative AVA and LVEF were 0.77 cm2 and LVEF 19%, respectively. About 15 of the 16 patients had postoperative echocardiograms available for comparison. And 7 of these 15 (47%) demonstrated improvement in LVEF to ≥20% (median LVEF 25%). The absence of coronary disease and improvement in AVA of ≥0.2 cm2 was associated with postoperative LVEF of ≥20%. Procedural mortality was 0%. The 30‐day, 6‐month, and 1‐year survival was 69%, 56%, and 29%. STS's mortality risk score ≥15% was associated with short‐term mortality.ConclusionWith appropriate technique, BAV can be reasonably safe in patients with LVEF <20%. Roughly half of these patients demonstrated improvement in LVEF to ≥20%. © 2013 Wiley Periodicals, Inc.
Percutaneous edge‐to‐edge repair of the mitral valve in patients with degenerative versus functional mitral regurgitation
Authors: Braun Daniel , Lesevic Hasema , Orban Martin , Michalk Fabian , Barthel Petra , Hoppe Katharina , Sonne Carolin , Pache Jürgen , Mehilli Julinda , Kastrati Adnan , Hausleiter Jörg , Massberg Steffen 
Published: December 2013

ObjectivesTo prospectively assess the outcome of percutaneous edge‐to‐edge repair in patients with degenerative versus functional mitral regurgitation (MR).BackgroundThe optimal patient population eligible for percutaneous edge‐to‐edge repair has yet to be defined.MethodsWe analyzed 119 patients treated by percutaneous edge‐to‐edge repair for symptomatic MR, 72 patients with degenerative and 47 patients with functional MR. The primary endpoints were defined as procedural success (MR grade reduction ≥1 grade) as well as a composite endpoint defined as freedom from MR 3+ or 4+, mitral valve reintervention and death 12 months after clip implantation. In patients with successful clip placement we further analyzed MR grade, New York Heart Association (NYHA) functional class, distance in the 6 min walking test and left ventricular volumes 12 months after clip implantation.ResultsThe primary success rate of all intended clipping procedures was 83.3% for degenerative and 89.4% for functional MR (P = 0.42). Regarding the composite endpoint we observed an event free survival of 59.7% in patients treated for degenerative MR and 63.8% in patients treated for functional MR (P = 0.73). We observed a highly significant reduction in MR grade as well as improvement in NYHA functional status in both groups 12 months after clip implantation. However, there was a more pronounced MR grade reduction in patients treated for degenerative MR compared with patients treated for functional MR.ConclusionsPercutaneous edge‐to‐edge repair of the mitral valve is feasible and comparably effective in patients with degenerative and functional MR. © 2013 Wiley Periodicals, Inc.
Detailed Mathematical Analysis of Solute Transport in Armfield's Liquid Diffusion Apparatus
Authors: Ramírez Carlos A. 
Published: December 2013

AbstractA solute's diffusivity is a key property in the design and analysis of mass transfer systems. A simple method to determine such coefficients in aqueous media is Armfield's diffusion apparatus, in which reservoir (donor) and bath (receiver) compartments are separated by a honeycomb array of liquid‐filled cylindrical pores. The solute of interest, i.e., NaCl, diffuses from the reservoir and pores into the bath. The electrical conductivity of the bath solution, which is proportional to the NaCl molarity, is tracked as a function of time at constant temperature. A comprehensive mathematical model is presented which combines simultaneous solute transport in the three compartments, allowing estimation of the aqueous NaCl diffusivity from experimental data. The model improves existing theoretical analyses of such data and can be adapted to study other challenging systems.
A Magnetic Ionic Liquid Based on Tetrachloroferrate Exhibits Three‐Dimensional Magnetic Ordering: A Combined Experimental and Theoretical Study of the Magnetic Interaction Mechanism
Authors: García‐Saiz Abel , Migowski Pedro , Vallcorba Oriol , Junquera Javier , Blanco Jesús Angel , González Jesús Antonio , Fernández‐Díaz María Teresa , Rius Jordi , Dupont Jairton , Rodríguez Fernández Jesús , de Pedro Imanol 
Published: December 2013

AbstractA new magnetic ionic liquid (MIL) with 3D antiferromagnetic ordering has been synthetized and characterized. The information obtained from magnetic characterization was supplemented by analysis of DFT calculations and the magneto‐structural correlations. The result gives no evidence for direct iron‐iron interactions, corroborating that the 3D magnetic ordering in MILs takes place via super‐exchange coupling containing two diamagnetic atoms intermediaries.
The case for colocation of high performance computing workloads
Authors: Breslow Alex D. , Porter Leo , Tiwari Ananta , Laurenzano Michael , Carrington Laura , Tullsen Dean M. , Snavely Allan E. 
Published: December 2013

SummaryThe current state of practice in supercomputer resource allocation places jobs from different users on disjoint nodes both in terms of time and space. While this approach largely guarantees that jobs from different users do not degrade one another's performance, it does so at high cost to system throughput and energy efficiency. This focused study presents job striping, a technique that significantly increases performance over the current allocation mechanism by colocating pairs of jobs from different users on a shared set of nodes. To evaluate the potential of job striping in large‐scale environments, the experiments are run at the scale of 128 nodes on the state‐of‐the‐art Gordon supercomputer. Across all pairings of 1024 process network‐attached storage parallel benchmarks, job striping increases mean throughput by 26% and mean energy efficiency by 22%. On pairings of the real applications Gyrokinetic Toroidal Code (GTC), Large‐scale Atomic/Molecular Massively Parallel Simulator (LAMMPS), and MIMD Lattice Computation (MILC) at equal scale, job striping improves average throughput by 12% and mean energy efficiency by 11%. In addition, the study provides a simple set of heuristics for avoiding low performing application pairs. Copyright © 2013 John Wiley & Sons, Ltd.