bluuebunny/crossref_metadata_2025_split huggingface dataset:
L3MBTL2 maintains MYCN‐amplified neuroblastoma cell proliferation through silencing NRIP3 and BRME1 genes
Authors: Okada Ryu , Takenobu Hisanori , Satoh Shunpei , Sugino Ryuichi P. , Onuki Ritsuko , Haruta Masayuki , Mukae Kyosuke , Nakazawa Atsuko , Akter Jesmin , Ohira Miki , Kamijo Takehiko 
Published: August 2024

AbstractEpigenetic alterations critically affect tumor development. Polycomb‐group complexes constitute an evolutionarily conserved epigenetic machinery that regulates stem cell fate and development. They are implicated in tumorigenesis, primarily via histone modification. Polycomb repressive complex 1 (PRC1) complexes 1–6 (PRC1.1–6) mediate the ubiquitination of histone H2A on lysine 119 (H2AK119ub). Here, we studied the functional roles of a PRC1.6 molecule, L3MBTL2, in neuroblastoma (NB) cells. L3MBTL2‐knockout and knockdown revealed that L3MBTL2 depletion suppressed NB cell proliferation via cell‐cycle arrest and gamma‐H2A.X upregulation. L3MBTL2‐knockout profoundly suppressed xenograft tumor formation. Transcriptome analysis revealed suppressed cell‐cycle‐related and activated differentiation‐related pathways. Break repair meiotic recombinase recruitment factor 1 (BRME1) and nuclear receptor interacting protein 3 (NRIP3) were notably de‐repressed by L3MBTL2‐knockout. The deletion of L3MBTL2 reduced enrichment of H2AK119ub and PCGF6 at transcriptional start site proximal regions of the targets. Add‐back studies unveiled the importance of L3MBTL2‐BRME1 and ‐NRIP3 axes for NB cell proliferation. We further manifested the association of MYCN with de‐repression of NRIP3 in an L3MBTL2‐deficient context. Therefore, this study first revealed the significance of L3MBTL2‐mediated gene silencing in MYCN‐amplified NB cells.
Differences and similarities in patient‐reported outcomes among men and women with haemophilia
Authors: Kempton Christine L. , Guasch Sara A. , Buckner Tyler W. , Mattis Shanna , Fedewa Stacey A. 
Published: August 2024

AbstractIntroductionBoth men and women can be diagnosed with haemophilia and the experience with haemophilia may be different between men and women.AimThis study aimed to compare patient‐reported outcomes in men versus women with haemophilia.MethodsThis cross‐sectional study is a post‐hoc analysis of data collected as part of the Haemophilia‐related Distress Questionnaire validation study. Adults aged ≥18 years with haemophilia A or B were recruited from one of two haemophilia treatment centres between July 2017 and December 2019. Outcomes included quality of life, measures of mental and physical health, and overall health. Unadjusted and multivariable linear regression models were used to examine potential mediators of sex‐based differences in outcomes.ResultsOf the 139 study participants included (21 women, 118 men), the mean age was 36.9 years and most (89.2%) had haemophilia A. Approximately 85.7% and 26.3% of women and men had mild haemophilia, respectively. PHQ‐9 depression and PROMIS‐29 Profile anxiety and fatigue scores were significantly higher in women than men in unadjusted and adjusted analyses. There were no statistically significant differences in other outcomes.ConclusionsWomen with haemophilia are more likely to experience depression, anxiety, and fatigue than men with haemophilia. This study highlights the need for mental health services to be integrated into the care of women with haemophilia. Future research is needed to understand whether women with haemophilia are more or less likely to experience depression, anxiety, and fatigue than women without haemophilia as well as determine the impact of reduced mental health on clinical outcomes.
Public Engagement in Health Policy‐Making for Older Adults: A Systematic Search and Scoping Review
Authors: You Jeonghwa , Ganann Rebecca , Wilson Michael , Carusone Soo Chan , MacNeil Maggie , Whitmore Carly , Dafel Andrea , Dhamanaskar Roma , Ling Eugenia , Dingman Lance , Falbo A. Tina , Kirk Michael , Luyckx Joyce , Petrie Penelope , Weldon Donna , Boothe Katherine , Abelson Julia 
Published: August 2024

ABSTRACTIntroductionAs the world's population ages, there has been increasing attention to developing health policies to support older adults. Engaging older adults in policy‐making is one way to ensure that policy decisions align with their needs and priorities. However, ageist stereotypes often underestimate older adults' ability to participate in such initiatives. This scoping review aims to describe the characteristics and impacts of public engagement initiatives designed to help inform health policy‐making for older adults.MethodsA systematic search of peer‐reviewed and grey literature (English only) describing public engagement initiatives in health policy‐making for older adults was conducted using six electronic databases, Google and the Participedia website. No geographical, methodological or time restrictions were applied to the search. Eligibility criteria were purposefully broad to capture a wide array of relevant engagement initiatives. The outcomes of interest included participants, engagement methods and reported impacts.ResultsThis review included 38 papers. The majority of public engagement initiatives were funded or initiated by governments or government agencies as a formal activity to address policy issues, compared to initiatives without a clear link to a specific policy‐making process (e.g., research projects). While most initiatives engaged older adults as target participants, there was limited reporting on efforts to achieve participant diversity. Consultation‐type engagement activities were most prevalent, compared to deliberative and collaborative approaches. Impacts of public engagement were frequently reported without formal evaluations. Notably, a few articles reported negative impacts of such initiatives.ConclusionThis review describes how public engagement practices have been conducted to help inform health policy‐making for older adults and the documented impacts. The findings can assist policymakers, government staff, researchers and seniors' advocates in supporting the design and execution of public engagement initiatives in this policy sector.Patient or Public ContributionOlder adult partners from the McMaster University Collaborative for Health and Aging provided strategic advice throughout the key phases of this review, including developing a review protocol, data charting and synthesis and interpreting and presenting the review findings. This collaborative partnership was an essential aspect of this review, enhancing its relevance and meaningfulness for older adults.
Emerging evidence on the potential of PTR‐MS as rapid, direct and high‐sensitivity sensors to promote innovation in the fermented beverages sector
Authors: Corvino Antonia , Silcock Pat , Capozzi Vittorio , Biasioli Franco 
Published: August 2024

SummaryIn food technology, the term ‘fermentation’ encompasses a spectrum of microbial‐driven bioprocesses that preserve and improve the quality of raw materials, transforming them into new fermented products with minimal resource consumption. Furthermore, fermentation plays a crucial role in driving the eco‐friendly transformation of the agricultural industry. Within this domain, the fermented beverage sector stands out due to its consumer‐friendly appeal. To promote innovation in this field, there is a need for cost‐effective, versatile and sustainable techniques that can accelerate research and development activities. In the bioprocess sector, innovation management requires consideration of an array of variables, including diverse raw materials, various microorganisms and different fermentation parameters. Direct injection mass spectrometry (DIMS) technologies offer solutions for bioprocess monitoring, particularly in the analysis of volatile organic compounds (VOCs) and are constantly improving their performance in terms of sensitivity and specificity. Among the DIMS methods, proton transfer reaction‐mass spectrometry (PTR‐MS), when coupled with autosampling and customised data handling and analysis, has demonstrated its efficiency in studying VOCs associated with fermentation. This approach brings automation to data production and management, offers exceptional versatility akin to a sensor and aligns closely with the principles of green chemistry. In this perspective paper, after reviewing key aspects of modern fermentation practice, we showcase the application of PTR‐MS as a model to demonstrate its potential as sensor‐like approach to drive innovation within the fermented beverage sector. This approach enables swift, large‐scale assessments of multiple variables while providing comprehensive insights into the quality and safety of the final products.
Effects of freeze–thaw pre‐treatment with different freezing methods on the microwave freeze drying of carrots
Authors: Li Linlin , Chen Xinyi , Cao Weiwei , Liu Wenchao , Duan Xu , Ren Guangyue , Song Chunfang 
Published: August 2024

SummaryThree freeze–thaw (FT) pre‐treatments using different freezing methods that freezing at −80 °C (FT‐80), freezing at −25 °C (FT‐25) and vacuum freezing (VFT) were used for carrot slices, the effects and mechanisms of FT pre‐treatment on the drying characteristics and product quality of carrots during microwave freeze drying (MFD) were studied. The results showed that FT pre‐treatment under different freezing methods affected the water state of carrots, resulting in varying degrees of moisture loss and shortening drying time by 14.3% to 42.8%. VFT treatment caused the greatest water loss (35.33%) during FT and significantly increased the effective moisture diffusivity in the MFD process. FT treatments did not cause any significant degradation in the colour of the carrots. The VFT and FT‐80 treatments resulted in an elevated rehydration ratio, increased crispiness of MFD carrots and products with a porous microstructure and higher porosity. In contrast, the FT‐25 treatment led to a decrease in porosity due to destructive damage to the carrot cell structure. However, this damage favoured the extractability of carotenoids, resulting in a higher carotenoid content than the other treatments. This study provides a theoretical foundation for the practical implementation of FT pre‐treatment using various freezing methods on fresh foods prior to drying. It is recommended to employ VFT pre‐treatment before MFD due to its efficiency and effectiveness.
Marijuana Legalization and Truck Safety
Authors: Balthrop Andrew , Phares Jonathan , Gordon Ron , Scott Alex 
Published: August 2024

ABSTRACTMany states have legalized medical and recreational marijuana use in the past decade, which has potential consequences for roadway safety. Using a state‐level panel of heavy truck crash statistics from 2005 to 2021 and a difference‐in‐difference estimation strategy, we test whether legalization has affected crash rates. Our results show that legalization does not increase crash rates on average; however, responses are heterogeneous across states, with Colorado, New Jersey, and Washington (state) showing crash reductions, while Connecticut and Virginia saw crashes increase. These results suggest that marijuana legalization does not have a straightforward relationship with heavy truck accidents, warranting continued research and policy scrutiny.
Ventricular tachycardia ablation with pentaspline pulsed field technology in two patients with ischemic cardiomyopathy
Authors: Fassini Gaetano , Zito Elio , Bianchini Lorenzo , Tundo Fabrizio , Tondo Claudio , Schiavone Marco 
Published: August 2024

AbstractIntroductionDue to its unique features, pulsed field ablation (PFA) could potentially overcome some limitations of current radiofrequency (RF) ventricular tachycardia (VT) ablation. However, data on the use of PFA in this setting are currently scarce.MethodsTwo patients with ischemic cardiomyopathy and previously failed RF VT ablations were treated with PFA.ResultsA total of 18 bipolar applications (case1) and seven bipolar applications (case2) were delivered to the infero‐lateral and infero‐septal areas (case1) and to the apical lateral left ventricular (LV) wall (case2), placing the catheter adjacent to the LV wall in the flower configuration. A rapid cessation of VT and restoration of sinus rhythm were observed during PFA delivery in both cases. Further applications were delivered to achieve complete elimination of late potentials. In case 1, during the in‐hospital stay, ECG monitoring did not show VT recurrences. Six‐month follow‐up was uneventful, with no VT recurrences at ICD interrogation. In case 2, due to postdischarge VT recurrences, a second RF procedure was scheduled 1 month later. The voltage map performed in sinus rhythm showed a low‐voltage zone located at the anterolateral wall, near the previous ablation site. Numerous late potentials were recorded. At the 6‐month follow‐up, no further VT recurrences were documented after RF redo ablation.ConclusionWhile the speed of application and potential transmural effect can facilitate the ablation of large diseased endocardial areas, early loss of contact due to difficult pentaspline catheter manipulation in the LV could lead to insufficient contact force and, consequently, inadequate energy penetration.
Blood pressure behavior during exercise in patients with diastolic dysfunction and a hypertensive response to exercise
Authors: Würzburger Laura , Stouwe Jan Gerrit van der , Ghidoni Céline , Wiech Patrick , Moser Georg , Petrasch Gloria , Schweiger Victor , Bohm Philipp , Rossi Valentina A. , Templin Christian , Caselli Stefano , Schmied Christian M. , Niederseer David 
Published: August 2024

AbstractA hypertensive response to exercise is a precursor leading to hypertension, which is a major risk factor for the development of heart failure and diastolic dysfunction. Herein, we aimed to assess blood pressure (BP) in patients with a hypertensive response to exercise and different degrees of diastolic dysfunction. Between January 2009 and December 2014, 373 patients with a hypertensive response to exercise (HRE) and echocardiographic data assessing diastolic function were enrolled at the University Hospital of Zurich. ANCOVA was used to assess the changes in BP response during exercise testing in individuals with different degrees of diastolic dysfunction. Normalization of systolic BP was blunted in patients with grade II and III diastolic dysfunction after 3 min of recovery in univariable [β (95%) ‐ 9.2 (‐13.8 to ‐ 4.8) p < .001, ‐16.0 (‐23.0 to 9.0) p < .001, respectively] and adjusted models. In fully adjusted models, when taking maximal effort into account, there were no differences with regard to systolic BP during exercise. Patients without diastolic dysfunction achieved higher heart rates (HRs) [both in absolute terms (p < .001) and as a percentage of the calculated maximum (p = .003)] and greater wattage (p < .001) at maximum exertion. The findings of this cross‐sectional study suggest that exercise capacity is compromised in patients with diastolic dysfunction. A hypertensive response to exercise and the finding of a blunted BP recovery may help identify patients at risk of developing heart failure.
Time‐varying copula‐based compound flood risk assessment of extreme rainfall and high water level under a non‐stationary environment
Authors: Song Mingming , Zhang Jianyun , Liu Yanli , Liu Cuishan , Bao Zhenxin , Jin Junliang , He Ruimin , Bian Guodong , Wang Guoqing 
Published: August 2024

AbstractQuantifying flood risk depends on accurate probability estimation, which is challenging due to non‐stationarity and the combined effects of multiple factors in a changing environment. The threat of compound flood risks may spread from coastal areas to inland basins, which have received less attention. In this study, a framework based on time‐varying copulas was introduced for the treatment of compound flood risk and bivariate design in non‐stationary environments. Archimedean copulas were developed to diagnose the non‐stationary trends of flood risk. Return periods, average annual reliabilities, and bivariate designs were estimated. Model uncertainty was analyzed by comparing the results for stationary and non‐stationary conditions. The case study investigated the extreme rainfall and water level series from the Qinhuai River Basin and the Yangtze River in China. The results showed that marginal distributions and correlations are non‐stationary in all bivariate combinations. Ignoring composite effects may lead to inappropriate quantification of flood risk. Excluding non‐stationarity may lead to risk over or underestimation. It showed the limitations of the 1‐day scale and quantified the uncertainty of non‐stationary models. This study provided a flood risk assessment framework in a changing environment and a risk‐based design technique, which is essential for climate change adaptation and water management.
One year of COVID‐19: A longitudinal study of individual and relational factors of psychological adjustment of individuals living in a romantic relationship in Portugal during the COVID‐19 pandemic
Authors: Relvas Ana Paula , Portugal Alda , Lacomba‐Trejo Laura , Major Sofia , Sotero Luciana , Agostinho Rafaela , Moreira Marta , Randall Ashley K. 
Published: August 2024

AbstractThis longitudinal study explored the impact of COVID‐19 on individuals in romantic relationships. The sample spans three waves: first confinement (n = 52), deconfinement (n = 49), and second confinement (n = 26). The study tested sociodemographic factors, psychological adjustment (anxiety, depression, stress, well‐being), COVID‐19 threat perception, dyadic coping, and relationship quality. Results from repeated measures ANOVA and fuzzy set qualitative comparative analysis (fsQCA) models revealed a decline in anxiety, depression, and stress symptoms, coupled with improved relationship quality over time. Well‐being and dyadic coping remained stable, while COVID‐19 threat perception increased. QCA models emphasized the predictive power of initial mental states (anxiety, depression, stress, and well‐being at W1) on subsequent adjustment. Notably, shorter relationship duration, healthcare worker status, and not having children, when combined with prior mental states, explained increased symptoms and diminished well‐being. The study underscores the significance of addressing these factors in individuals navigating romantic relationships during the pandemic.
Evaluating the combination of in‐person and electronic social networking services for family caregivers of stroke survivors: A quasi‐experimental analysis
Authors: Kuo Wen‐Yu , Chen Chen‐Yin , Wang Jeng , Wang Chin‐Man , Chen Min‐Chi , Chang Ting‐Yu 
Published: August 2024

AbstractIntroductionThe effectiveness of health interventions delivered via a combination of in‐person and electronic social networking services for caregivers of stroke survivors remains uncertain. This study evaluates the feasibility of implementing educational and peer support programs for these caregivers through such platforms.DesignQuasi‐experimental design.MethodsThis study included 105 caregiver–survivor dyads, with 54 dyads allocated to the intervention group and the remaining 51 to the control group. The LINE intervention comprised a combination of in‐person and electronic social networking services including stroke and rehabilitation education, problem‐solving skills training, long‐term care information support, and 24‐h peer and professional support for caregivers. The outcomes were assessed at baseline, after 1 month, and after 3 months, and encompassed caregivers' care burden, depressive symptoms, perceived social support, and quality of life, as well as the rehabilitation adherence and depressive symptoms of stroke survivors. Generalized estimating equations were used to examine group differences. The data were collected between August 2021 and October 2022.ResultsThe average age of the caregivers was 48.3 years. Caregivers in the intervention group reported reduced care burdens and enhanced perceptions of social support and quality of life as compared to those in the control group. Additionally, stroke survivors in the intervention group were less likely to exhibit high‐risk depressive symptoms.ConclusionDelivering a stroke caregiver support intervention via in‐person and electronic social networking services, such as LINE, effectively reduced the care burden for caregivers of stroke survivors. Additionally, it enhanced caregivers' perceived social support and quality of life.Clinical RelevanceThis study demonstrated that caregiver education and peer support programs administered through a combination of in‐person and electronic social networking services can serve as an effective support system for the psychosocial health of stroke caregivers. These findings support the integration of such interventions into standard clinical practice by healthcare providers or governmental bodies.
Artificial intelligence in cosmetic dermatology
Authors: Kania Barbara , Montecinos Karen , Goldberg David J. 
Published: August 2024

AbstractBackgroundCosmetic dermatology is a growing field as more patients are seeking treatments for esthetic concerns. Traditionally, practitioners and patients utilize their own perceptions, current beauty standards, and manual observation to determine their satisfaction with cosmetic interventions. Artificial intelligence (AI) can be introduced into cosmetic dermatology to provide objective data‐driven recommendations to both dermatologists and patients.ObjectiveThe purpose of this paper is to compose a unified review that illustrates the various facets of artificial intelligence and formulate a hypothesis regarding the new implications of artificial intelligence in cosmetic dermatology specifically.MethodsA comprehensive search on PubMed was conducted to identify the available information related to AI in cosmetic dermatology. The search was conducted using a combination of keywords including “cosmetic dermatology” and “artificial intelligence.”ResultsThe current literature indicates that AI models offer personalized, efficient, and result‐driven outputs that can enhance cosmetic outcomes, patient satisfaction, and overall experience.ConclusionArtificial intelligence integration in cosmetic dermatology shows a promising future, offering the ability to analyze vast data sets and deliver a tailored patient experience. By incorporating AI into cosmetic dermatology, there is an opportunity to balance evidence‐based decision‐making with the artistic human touch of cosmetic dermatologists.