bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic variants in IRF2, STAT6, and TSLP are associated with eczema herpeticum in a European cohort of patients with atopic dermatitis
Authors: Zeitvogel Jana , Klug Ilona , Traidl Stephan , Rösner Lennart , Mommert Susanne , Döhner Katinka , Werfel Thomas 
Published: July 2024

Abstract

Eczema herpeticum (EH) is a disseminated severe herpes simplex virus infection that occurs in a subset of patients with atopic dermatitis (AD). EH is a complex multifactorial disease caused by immunological changes, environmental influences, and genetic aberrations. The latter is becoming increasingly apparent, and several single nucleotide polymorphisms (SNP) have been associated with triggering EH, including genes related to interferon signaling, the epidermal barrier, and Th2-mediated immunity. So far, genetic studies have not considered the severity of AD, which may have led to associations related to AD severity rather than EH. To investigate genetic risk factors for EH in a European cohort, we analyzed several SNPs of the genes STAT6, IFNG, IFNGR1, IRF2, and TSLP in AD patients with (ADEH+) versus a carefully matched control group of AD patients consisting of 44 patients matched for age, sex, and severity of AD (SCORAD) without a history of eczema herpeticum (ADEH-) by pyrosequencing. We confirmed an association of rs2416259 (TSLP), rs167769 (STAT6), and rs11132242 (IRF2) with ADEH + in our European cohort. However, the risk alleles for rs167769 and rs11132242 were contrary to previous reports that did not take age, sex, and disease severity into account. We could not confirm an association for several loci (rs3024975 (STAT6); rs2069705, rs2069718, rs2069727, and rs2430561 (IFNG); rs3799488 and rs9376269 (IFNGR1); rs1342852 (IRF2)) previously described in other cohorts. Moreover, linkage disequilibrium (LD) analysis revealed gametic LD and epistatic effects between STAT6, IFNGR, and IFNG genes. Better knowledge of genetic factors predisposing to eczema herpeticum may allow the early identification of patients at increased risk and disease prevention. Our study provides important clues to possible key factors in the antiviral immunity in herpes simplex virus infection and thus to potential therapeutic interventions.
Single-cell analysis reveals immune cell abnormalities underlying the clinical heterogeneity of systemic sclerosis
Authors: Nishide Masayuki , Shimagami Hiroshi , Nishimura Kei , Matsushita Hiroaki , Metsugi Shoichi , Kato Yasuhiro , Kawasaki Takahiro , Tsujimoto Kohei , Edahiro Ryuya , Itotagawa Eri , Naito Maiko , Kawada Shoji , Nakatsubo Daisuke , Matsukawa Kazuki , Namba-Hamano Tomoko , Inoue Kazunori , Takahashi Atsushi , Mizui Masayuki , Omiya Ryusuke , Isaka Yoshitaka , Okada Yukinori , Hattori Kunihiro , Narazaki Masashi , Kumanogoh Atsushi 
Published: July 2024

Abstract

Autoimmune rheumatic diseases present with diverse clinical manifestations that often complicate management strategies. Systemic sclerosis (SSc) is a representative disease with multiple organ manifestations affecting patients worldwide, and exploring the variation of immune abnormalities in this disease is of great interest. However, previous studies have focused on diseased tissues, and it remains largely unknown how cellular diversity links to clinical heterogeneity. Here, we perform single-cell transcriptome and surface proteome analyses of peripheral blood mononuclear cells (PBMCs) from 21 SSc patients who are not receiving immunomodulatory therapy and show that different clinical manifestations are associated with distinct immune abnormalities. Enrichment of a specific CD14+ monocyte subset characterized by EGR1 expression is observed in patients with scleroderma renal crisis (SRC). Integrated analysis of PBMCs and kidney biopsy cells indicates that this monocyte subset directly differentiates into tissue-damaging macrophages under activation of NF-κB signaling. Clinically, EGR1 expression in monocytes is significantly upregulated at the onset of SRC and decreases after treatment, suggesting its potential as a biomarker for SRC. In patients with interstitial lung disease (ILD), a CD8+ T cell subset with type II interferon signature is highly enriched in both peripheral blood and lung tissue of patients with progressive disease, suggesting that chemokine-driven migration of these cells is involved in ILD progression. Thus, distinct immune cell profiles at the single cell level reveal different directions of immune dysregulation between organ manifestations and provide insights for tailored treatment strategies.
UVR Prediction Over Lagos, Ibadan and New Richmond for Public Health and Renewable Energy
Authors: OYEDOKUN Sherifdeen M. , OLATONA Gbadebo I. , ADISA Shuaib A. 
Published: July 2024

Abstract

Ultraviolet radiation (UVR) is a significant environmental factor influencing various biological and chemical processes, including photosynthesis in plants, vitamin D synthesis in humans, and microbial sterilization. However, excessive UVR exposure can lead to adverse effects such as skin cancer and DNA damage. This study applies the ARIMA (AutoRegressive Integrated Moving Average) model to predict UVR levels over Lagos and Ibadan in Nigeria, and New Richmond in the United States, utilizing a 21-year dataset spanning from 2000 to 2020. By analysing the autocorrelation function (ACF) and partial autocorrelation function (PACF) with a significance level of 0.25, the stationarity and appropriate parameters for the ARIMA model were identified. The model is then used to predict daily UVR values for the last day of each month from January 2021 to December 2022. Results indicate that the ARIMA model effectively captures the temporal patterns in UVR data, with validation metrics such as RMSE, MAE, and MAPE confirming its predictive accuracy. This predictive capability can inform public health advisories, agricultural practices, and environmental management, emphasizing the need for ongoing monitoring and prediction of UVR levels to mitigate potential health risks.
Meta-learning applied to a multivariate single-step fusion model for greenhouse gas emission forecasting in Brazil
Authors: Enamoto Liriam Michi , Santos Andre Rufino Arsenio , Weigang Li , Meneguette Rodolfo , Rocha Filho Geraldo P. 
Published: July 2024

ABSTRACT

Climate change, driven by greenhouse gas (GHG) emissions, causes extreme weather events, impacting ecosystems, biodiversity, population health, and the economy. Predicting GHG emissions is crucial for mitigating these impacts and planning sustainable policies. This research proposes a novel machine learning model for GHG emissions forecasting. Our model, Meta-Learning Applied to Multivariate Single-Step Fusion Model, utilizes historical GHG emissions from Brazil over the past 60 years to predict CO2 and CH4 emissions. Additionally, the model employs a unique combination of two techniques in time series forecasting: (i) in the Fusion Model, each substance is individually extracted and trained based on a specific decision task, then integrated into the same feature space; (ii) Meta-Learning allows the model to learn from past prediction tasks, leading to better generalization. Our model was compared with state-of-the-art time series models using the same dataset. The results show that our approach reduces the mean absolute percentage error by 49.06% with 95% confidence compared to the Transformer-based TST model, demonstrating its superior performance and low estimated CO2 emissions of 0.01 kg CO2eq. Furthermore, the model's flexibility allows it to be adapted for various environmental studies and general time series forecasting.
Shape Dependent Therapeutic Potential of Nanoparticulate System: Advance Approach for Drug Delivery
Authors: Verma Shristy , Malviya Rishabha , Srivastava Saurabh , Ahmad Irfan , Singh Bhupinder , Almontasheri Rasha , Uniyal Prerna 
Published: July 2024

:
Drug delivery systems rely heavily on nanoparticles because they provide a targeted and monitored
release of pharmaceuticals that maximize therapeutic efficacy and minimize side effects. To maximize drug internalization,
this review focuses on comprehending the interactions between biological systems and nanoparticles.
The way that nanoparticles behave during cellular uptake, distribution, and retention in the body is determined
by their shape. Different forms, such as mesoporous silica nanoparticles, micelles, and nanorods, each
have special properties that influence how well drugs are delivered to cells and internalized. To achieve the desired
particle morphology, shape-controlled nanoparticle synthesis strategies take into account variables like
pH, temperatures, and reaction time. Top-down techniques entail dissolving bulk materials to produce nanoparticles,
whereas bottom-up techniques enable nanostructures to self-assemble. Comprehending the interactions
at the bio-nano interface is essential to surmounting biological barriers and enhancing the therapeutic efficacy
of nanotechnology in drug delivery systems. In general, drug internalization and distribution are greatly influenced
by the shape of nanoparticles, which presents an opportunity for tailored and efficient treatment plans in
a range of medical applications.
A Novel Tryptanthrin Derivative D6 Induces Apoptosis and DNA Damage in Non-small-cell Lung Cancer Cells Through Regulating the EGFR Pathway
Authors: Long Haitao , Zhang Guanglong , Zhou Yue , Qin Liqing , Zhu Danxue , Chen Jiayi , Liu Bo , Tan Huayuan , Chen Danping , Li Zhurui , Li Chengpeng , Wang Zhenchao 
Published: July 2024

Background:
Non-small-cell lung cancer is a prevalent malignancy associated with significant morbidity
and mortality rates. Tryptanthrin and its derivatives have exhibited potent antitumor activity.

Objective:
This study aims to investigate the inhibitory effect of a novel synthesized tryptanthrin derivative D6 on
proliferation and the possible mechanism of human non-small cell lung cancer cell lines (A549) in vitro.

Methods:
In this study, MTT assay, cell migration, colony formation assay, cell cycle analysis, cell apoptosis, JC-
1 staining assay, reactive oxygen species analysis, proteomics, western blotting, high content screening and absorption
titrations analysis were performed.

Results:
We found that D6 inhibited both the proliferation and migration, induced cell cycle arrest in the G2/M
phase, increased levels of ROS, decreased mitochondrial membrane potential, and promoted apoptosis in A549
cells. Further mechanistic studies found that D6 reduced EGFR expression in A549 cells and inhibited the EGFR
pathway by decreasing phosphorylation levels of EGFR, Stat3, AKT and Erk1/2. Moreover, DNA damage induced
by D6 involved an increase in p53/MDM2 ratio and concentration-dependent accumulation of micronuclei.

Conclusion:
D6 demonstrated significant antitumor activity against A549 cells by inhibiting the EGFR signaling
pathway, inducing DNA damage, and subsequently leading to oxidative stress, apoptosis, and cell cycle arrest.
Our findings suggest that D6 exhibits potential as an NSCLC drug, owing to its attributes such as antiproliferative
activity and ability to induce apoptosis by attenuating the EGFR-mediated signaling pathway.
Cutting-Edge Developments and Patent Trends in Microspheres Drug
Delivery: A Comprehensive Overview
Authors: Sharma Shailesh , Pankaj  , Kumar Subhash , Sharma Neelam , Verma Surajpal 
Published: July 2024

Abstract:
Microspheres have emerged as innovative drug delivery platforms with significant potential
to improve the therapeutic efficacy of drugs with limited aqueous solubility and prolong their release.
This abstract provides an overview of recent developments in microsphere research, highlighting
key trends and innovative approaches. Recent studies have focused on various aspects of microspheres,
including formulation techniques, materials selection, and their applications in drug delivery.
Recent breakthroughs in polymer science have paved the way for the creation of innovative biodegradable
and biocompatible materials for microsphere fabrication, improving drug encapsulation
effectiveness and release dynamics. Notably, the integration of nanomaterials and functionalized
polymers has enabled precise control over drug release rates and enhanced targeting capabilities. The
utilization of microspheres for administering a diverse array of therapeutic substances, including anticancer
drugs, anti-inflammatory agents, and peptides, has gained significant attention. These microspheres
have demonstrated the potential to enhance drug stability, minimize dosing frequency and
enhance patient adherence.
Comparative Analysis and Characteristic Verification of the Quality Standard of Milk Thistle Extract
Authors: Zhao Xi , Lan Tao , Luo Shihui , Yu Congcong , Huang Jinli , Zhou Qi , He Ruikun 
Published: July 2024

Introduction::
Milk thistle extract is widely used in dietary supplements due to its strong
hepatoprotective effect. There are some differences in the quality characteristics and standard values
of milk thistle extract set by pharmacopoeias and standards in various countries and regions.

Method:
To promote the standardization of quality standards for milk thistle extract, this paper
summarized and compared the standard characteristics and related values settings in four different
standards. At the same time, actual samples were tested for the characteristics that appeared more
frequently in standard. We found that the ratio algorithm of silymarin set in the pharmacopoeia
standards was not reasonable and could blur the quality of related products from the test results, especially
without limiting the total amount of silymarin in the standard.

Results:
We suggested setting standard values for all six silymarin characteristics, establishing more
universal detection methods, and preparing relevant reference materials to match the detection methods.
Based on the comparison of standards and test data, the characteristics of the ethanol content,
ethyl acetate content, methanol insoluble substances, heavy metal content, and microbial characteristics
should draw more attention and should be considered in the future for setting newly formulated
standards.

Conclusion:
This paper lays the data foundation for developing higher quality standards for milk
thistle extract, which plays an important role in promoting the international trade of milk thistle extract.
Kelayakan Media Flashcard Sub Materi Manfaat Keanekaragaman Hayati Berbasis Tumbuhan Upacara Adat
Authors: Lorenza Fransiska Dena , Syamsiwna Syamsiwna , Fajri Hayatul 
Published: July 2024

Utilization of biodiversity includes the use of plants in the fields of clothing, shelter, food, cosmetics, medicine, economy, and culture. Activities that must be carried out by teachers and students on the Submatter of Biodiversity Utilization are analyzing observation data on various levels of biodiversity, threats and preservation. This study aims to determine the feasibility of flashcard media as an implementation of the ethnobotany results of the traditional ceremony of the Dayak Seberuang tribe of Kantuk Hulu Hamlet made for learning media on the Biodiversity Submaterial of Class X SMA. The research was conducted in 3 schools, namely Santun UNTAN High School, Taman Mulia High School and SMAN 2 Pontianak and at FKIP Tanjungpura University Pontianak. Flashcard media validation was carried out by 3 Biology teachers and 2 Biology Education Lecturers consisting of 5 aspects and 11 criteria. The validation results obtained a CVI value of 0.99. Flashcard media is declared valid or feasible to use as learning media.

Abstrak. Pemanfaatan keanekaragaman hayati termasuk pemanfaatan tumbuhan dalam bidang sandang, papan, pangan, kosmetik, obat-obatan, ekonomi, dan budaya. Kegiatan yang harus dilakukan oleh guru dan peserta didik pada Submateri Pemanfaatan Keanekaragaman Hayati adalah menganalisis data hasil observasi tentang berbagai tingkat keanekaragaman hayati, ancaman dan pelestariannya. Penelitian ini bertujuan untuk mengetahui kelayakan media flashcard sebagai implementasi dari hasil etnobotani upacara adat suku Dayak Seberuang Dusun Kantuk Hulu yang dibuat untuk media pembelajaran pada Submateri Keanekaragaman Hayati Kelas X SMA. Tempat penelitian dilakukan di 3 Sekolah yaitu di SMAS Santun UNTAN, SMAS Taman Mulia dan SMAN 2 Pontianak serta di FKIP Universitas Tanjungpura Pontianak. Validasi media flashcard dilakukan oleh 3 orang guru mata pelakaran Biologi dan 2 orang Dosen Pendidikan Biologi yang terdiri dari 5 aspek dan 11 kriteria. Hasil validasi mendapatkan nilai CVI 0,99. Media flashcard dinyatakan valid atau layak digunakan sebagai media pembelajaran.
Literature Review : Upaya Meningkatkan Literasi Visual Untuk Menstimulasi Kemampuan Berpikir Dalam Pembelajaran Biologi
Authors: Handayani Fitri , Fadilah Muhyiatul 
Published: July 2024

Visual literacy plays an important role in building a person's cognitive processes. Visual Literacy helps students understand concepts and improve memory and recall information. Understanding and interpreting visual messages is not easy, because they can have different meanings depending on the background of the person who sees them. The aim of this literature is to stimulate interest in the use of visual devices in the biology learning process and to develop students' visual literacy abilities. students must use visual, exploratory, critical, and reflective thinking skills. One way to train these thinking skills is to utilize the cognitive processes of the visuals used. Designing visual representations requires knowledge and skills. This relates to how the use of visual representations can support the process of developing thinking skills when learning biology.
abstrak. Literasi visual berperan penting dalam membangun proses kognitif seseorang. Literasi Visual mambantu siswa memahami konsep dan meningkatkan memori dan menngingat informasi. Memahami dan menafsirkan pesan visual tidaklah mudah, karena dapat memiliki arti yang berbeda-beda tergantung dari latar belakang orang yang melihatnya. Tujuan dari literatur ini adalah untuk merangsang minat penggunaan perangkat visual dalam proses pembelajaran biologi dan untuk mengembnagkan kemampuan literasi visual siswa. siswa harus menggunakan keterampilan berpikir visual, eksplorasi, kritis, dan reflektif. Salah satu cara untuk melatih keterampilan berpikir tersebut adalah dengan memanfaatkan proses kognitif dari visual yang dimanfaatkan. Merancang representasi visual membutuhkan pengetahuan dan keterampilan. Hal ini berkaitan tentang bagaimana penggunaan representasi visual dapat mendukung proses pengembangan keterampilan berpikir ketika pembelajaran biologi.
Manajemen Penetasan Telur Tetas Ayam Sentul (Studi Kasus di UPTD. Balai Pengembangan Perbibitan Ternak Unggas (BPPTU) Jatiwangi)
Authors: Suciati Bella Permata , Herlina Linda , Kuswaryan Sondi 
Published: July 2024

Indikator keberhasilan program pembibitan dalam manajemen penetasan ayam sentul di UPTD. Balai Pengembangan Perbibitan Ternak Unggas (BPPTU) Jatiwangi adalah fertilitas, daya tetas, dan salable chick. UPTD. BPPTU Jatiwangi merupakan balai yang bertanggung jawab atas pengembangan dan peningkatan bibit unggas lokal di bawah naungan Dinas Ketahanan Pangan dan Peternakan (DKPP) Provinsi Jawa Barat. Penelitian ini bertujuan untuk mengetahui fertilitas, daya tetas dan salable chick yang dihasilkan dari ayam sentul di BPPT Unggas Jatiwangi. Metode analisis yang digunakan adalah observasi dan data yang didapat dianalisa dengan statistika deskriptif. Penelitian dilakukan dengan studi kasus dengan diambil data primer dan sekunder. Telur tetas yang digunakan bobot 41-55 gr yang berasal dari UPTD. BPPTU Jatiwangi dengan perbandingan jantan dan betina 1:5. Parameter yang diamati adalah fertilitas, daya tetas, dan salable chick. Hasil penelitian menunjukan rataan fertilitas 81,62Â±1,34 % dengan koefisien variasi 1,64 %, daya tetas 74,64Â±4,94 % dengan koefisien variasi 6,61 % dan salable chick 96,4Â±1,65 % dengan koefisien variasi 1,71 %.
Hawa, Taman, dan Cinta: Metafora Religiositas pada Puisi-Puisi Sapardi Djoko Damono
Authors: Isnaini Heri , Lestari Riana Dwi 
Published: July 2024

AbstrakReligiositas menjadi tema yang sangat penting dalam puisi. Tema ini berkelindan mewujud bahasa figuratif dalam berbagai tataran, baik itu kata, frasa, kalimat, larik, bait, dan tipografi. Bahasa figuratif berjenis metafora menjadi bahasa yang paling banyak digunakan dalam puisi. Artikel ini bertujuan memaparkan penggunaan metafora religiositas pada puisi-puisi Sapardi Djoko Damono. Penggambaran metafora religiositas pada artikel ini diwujudkan dalam diksi “Hawa”, “taman”, dan “cinta”. Ketiganya merepresentasikan nilai-nilai religiositas pada tataran kepercayaan kepada Tuhan. Pembacaan metafora religiositas tersebut memanfaatkan penggunaan teori stilistika dan hermeneutika. Selain itu, pendekatan deskriptif analitik kualitatif diharapkan dapat mengejawantah dan mengelaborasi pemaknaan puisi secara lebih komprehensif. Dengan demikian, pembahasan ini diharapkan dapat memberikan pemaknaan atas nilai-nilai religiositas yang terdapat dalam puisi-puisi karya Sapardi Djoko Damono. Kata Kunci: hermeneutika, makna, metafora, puisi, religiositas AbstractReligiosity is a very important theme in poetry. This theme manifests figurative language in various levels, be it words, phrases, sentences, arrays, stanzas, and typography. Metaphorical figurative language became the most widely used language in poetry. This article aims to explain the use of religiosity metaphors in the poems of Sapardi Djoko Damono. The metaphorical depiction of religiosity in this article is embodied in the dictions "Eve", "garden", and "love". All three represent the values of religiosity on the level of belief in God. The reading of the metaphor of religiosity utilizes the use of the theories of stilistics and hermeneutics. In addition, descriptive approaches to qualitative analytics are expected to spell and elaborate the meaning of poetry more comprehensively. Thus, this discussion is expected to provide meaning to the values of religiosity contained in poems by Sapardi Djoko Damono. Keywords: hermeneutics, meaning, metaphor, poetry, religiosity
Analisis Penggunaan Dialek Bahasa Melayu Indragiri Hilir dalam Cerita Rakyat Lang Buana
Authors: Fauziah Fauziah , Yuliani Fitri , Vanditama Jery , Syaharani Latifah , Akmal Akmal 
Published: July 2024

AbstrakStudi ini berfokus pada analisis penggunaan dialek Bahasa Melayu Indragiri Hilir dalam cerita rakyat "Lang Buana". Dengan metode deskriptif kualitatif, penelitian ini mencoba untuk memahami dan menjelaskan fenomena kebahasaan dalam konteks sosial dan budaya masyarakat Indragiri Hilir. Hasil penelitian menunjukkan bahwa dialek Bahasa Melayu Indragiri Hilir berfungsi sebagai identitas dan media untuk melestarikan serta mengimplementasikan dialek dan budaya lokal kepada generasi muda. Berdasarkan penelitian yang telah dilakukan, Dialek Bahasa Melayu Indragiri Hilir tidak hanya berperan sebagai alat komunikasi, tetapi juga sebagai simbol identitas budaya dan sejarah. Penelitian ini berharap dapat memberikan kontribusi pada pelestarian dan pengembangan Bahasa Melayu dan dialek-dialeknya. Kata Kunci: dialek, bahasa Melayu, cerita rakyat AbstractThis study focuses on analyzing the use of the Indragiri Hilir Malay dialect in the folktale "Lang Buana". Using qualitative descriptive methods, this research tries to understand and explain linguistic phenomena in the social and cultural context of the Indragiri Hilir community. The research results show that the Indragiri Hilir Malay dialect functions as an identity and media to preserve and implement local dialects and culture to the younger generation. Based on research that has been conducted, the Indragiri Hilir Malay Dialect not only acts as a communication tool, but also as a symbol of cultural and historical identity. This research hopes to contribute to the preservation and development of the Malay language and its dialects. Keywords: Malay Dialects, Folklore