bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hair Growth on Nude Mice Due to Cyclosporin A
Authors: Watanabe Shinichi , Mochizuki Akihiko , Wagatsuma Keiko , Kobayashi Miyuki , Kawa Yohko , Takahashi Hisashi 
Published: April 2015

AbstractOne of the most common dermatological side effects of cyclosporin A (CsA) is dose‐dependent hypertrichosis. Similar hair growth was noted in nude mice in an attempt to increase the acceptance of human xenografts with CsA in the T‐cell‐deficient congenitally athymic nude (nu/nu) mice. The aim of the present study was to further investigate the stimulation of hair growth on nude mice not only by oral administration of CsA but also by topical and subcutaneous administration of CsA. Young BALB/c female nude mice were treated for 3 or 4 weeks with topical, oral, or subcutaneous applications of CsA dissolved in olive oil at various doses. The hair of CsA‐treated mice appeared to grow from 7 days after the treatment, even at low doses. Induced hair growth was dose‐dependent and became clearly obvious 3 weeks after the treatment. The stimulation of hair growth was not restricted to the site of topical application. The distribution of the new hair depended on the natural pattern of hair growth in the mice. However, there was no hair growth in the control mice which were given only olive oil. Histological examination revealed that there were no differences in the structures of skin and hair between the control and the CsA‐treated mice. Furthermore, the number of hair follicles did not remarkably increase after CsA treatment. The hair growth in the CsA‐treated mice stopped after cessation of the treatment and returned to the level of the control mice on day 14 after the end of the treatment. Subsequent retreatment with CsA resulted in further regrowth of the hair. However, old nude mice (9 months old) revealed no hair growth at the end of a 4‐week treatment with CsA.
Keratin Expression in Merkel Cells of Fetal Rat Skin
Authors: Nindl Margit , Nakagawa Hidemi , Ishibashi Yasumasa 
Published: April 2015

AbstractThe cytokeratin expression of Merkel cells in fetal rat skin was studied by light‐ and electron microscopy. Employing a pre‐embedding staining method, 2 monoclonal anti‐keratin antibodies (RCK‐102, MA‐902) were shown to stain Merkel cells specifically. Neighbouring keratinocytes were unstained. The staining reaction seems to be based on the expression of 52.5 kD cytokeratin.
AgNOR (Nucleolar Organizer Regions) Staining in Malignant Melanoma
Authors: Nagatani Tetsuo , Iemoto Gaijiro , Miyakawa Kanata , Ichiyama Shinichi , Takahashi Yasuhide , Uchiyama Mitsuaki , Nakajima Hiroshi 
Published: April 2015

AbstractNucleolar organizer regions (NORs) are loops of ribosomal DNA seen in nuclei, which are demonstrable as black dots (AgNOR) in tissue sections by silver (Ag) colloid staining. The number of such AgNORs is correlated with cellular activity and is an indicator of the degree of malignancy. In this study, 76 melanocytic lesions were analyzed by AgNOR staining, and the clinical and histopathological characteristics of malignant melanoma and melanocytic nevi were considered. Although the AgNOR counts for melanocytic nevi were significantly different from those in malignant melanoma, an obvious overlap between them was detected. The number of AgNORs in melanocytic nevi per cell was usually 1 or 2. On the other hand, the number of AgNORs per malignant melanoma cell was variable. Morphologically, malignant melanoma cells often showed dispersal of AgNORs throughout the nucleus as well as multiple nucleoli containing clustered AgNORs, whereas melanocytic nevus cells tended to have a regular nucleolus with tightly clustered AgNORs. The correlation between AgNOR count and pathological staging was uncertain, but a slight correlation between AgNOR count and thickness of the primary lesion was obtained. However, the AgNOR count in malignant melanoma was not a prognostic factor for the disease. Therefore, the AgNOR method is difficult to use for differential diagnosis between benign pigmented lesions and malignant melanoma. Nonetheless, an AgNOR count of more than two per cell favors a diagnosis of malignant melanoma.
Characterization of an Immortalized Human Cell Line Derived from Neonatal Foreskin Diploid Fibroblasts
Authors: Chiang Lien‐Chai , Chiang Wen , Chang Shui‐Feng , Chen Hour‐Young , Yu Hsin‐Su 
Published: April 2015

AbstractA new human skin cell line, designated as CCFS‐1/KMC, immortalized from human neonatal foreskin diploid fibroblast cells, has been subcultured successfully in vitro for more than 500 passages. This anchorage‐dependent cell line possesses many common features of transformation such as morphological and cytoskeletal changes, hypotriploidy, infinite lifespan, increasing plating efficiency and saturation density, and decreasing serum requirement and population doubling time. Human papillomavirus (HPV) type 18 DNA was detected in the cell line before and after immortalization by the polymerase chain reaction (PCR) method. Tumorigenicity, however, was not demonstrated in vivo. The isoenzyme activity of the cell line shows activation of a placental form of alkaline phosphatase and a changing lactate dehydrogenase isoenzyme pattern that is different from transformation by carcinogens. Class I HLA and class II HLA antigens are constitutively expressed on this skin cell line. Here we report that these immortalized human fibroblasts derived from neonatal HPV‐18‐DNA‐contained diploid fibroblasts possess double minute chromosomes (DMs), a karyotypic aberration usually found in cancer cells.
The Effect of Human Anaphylatoxins and Neutrophils on Histamine Release from Isolated Human Skin Mast Cells
Authors: Kubota Yasuo 
Published: April 2015

AbstractThe concept of mast cell heterogeneity has been studied extensively. Recently developed techniques to enzymatically disperse skin mast cells from human skin have shown that skin mast cells are somehow different from those of other organs such as lung and intestine. In this report, we have isolated and partially purified human skin mast cells from human neonatal foreskins by collagenase and hyaluronidase digestion. These mast cells are morphologically intact by histological, immunohistochemical and electron microscopic criteria. These human skin mast cells secrete histamine significantly (max. net histamine release, 20~30%) in a dose‐related, temperature‐ and time‐dependent fashion following stimulation with purified human C5a and C3a (over the ranges of 5 × 10–8 M to 10–7 M and 3 × 10–7 M to 6 × 10–6 M, respectively).On the other hand, interactions between human skin mast cells and other leukocytes have long been suspected of playing a very important role in cutaneous inflammation. Recently, a human neutrophil‐derived histamine‐releasing activity termed HRA‐N was partially purified. HRA‐N has been shown to cause human and rat basophil leukemia cells to degranulate. This study was also undertaken to assess the ability of HRA‐N to directly induce histamine release from isolated human skin mast cells. HRA‐N causes dose‐ and time‐dependent histamine release as do human anaphylatoxins. These results suggest that HRA‐N may lead to a better comprehension of allergic and inflammatory reactions and their modulation in the skin.
Churg‐Strauss Syndrome: Report of a Case without Preexisting Asthma
Authors: Chen Ko‐Ron , Ohata Yoshiyuki , Sakurai Misa , Nakayama Hideo 
Published: April 2015

AbstractA 38‐year‐old male with no history of asthma, who initially presented with episcleritis and subsequently developed symptoms of systemic vasculitis with marked blood eosinophilia, was diagnosed as having Churg‐Strauss syndrome (CSS). Both the patient's skin lesions and pulmonary infiltrates revealed histologically proven vasculitis with predominantly eosinophilic infiltration.All the symptoms of systemic vasculitis except the persisting peripheral neuropathy dramatically improved after corticosteroid was systemically used.Since scattered non‐asthmatic cases of CSS have been reported recently, and three such patients have died of acute heart failure due to delayed diagnosis, absence of asthma should not be regarded as an absolute criterion for exclusion of the diagnosis of CSS. Earlier recognition of this disease is important, since earlier institution of steroid therapy can prevent the acute onset of fatal cardiac involvement during the course of this disease.
The Effects of Interferon‐β on Phorbol Ester or Calcium Ionophore‐induced Intercellular Adhesion Molecule‐I Expression in Epidermal Carcinoma Cells
Authors: Fujisawa Hiroshi , Naito Yukiko , Horiuchi Sanae , Baba Toru , Otsuka Fujio 
Published: April 2015

AbstractKeratinocyte intercellular adhesion molecule (ICAM)‐I expression is induced by interferon (IFN)‐γ. It has been previously reported that IFN‐β suppresses IFN‐γ‐induced ICAM‐I expression in A431 cells, a human squamous cell carcinoma cell line. In this study, the suppresion mechanisms were investigated at the post second messenger level. Both 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) and calcium ionophore (A23187) induce ICAM‐I expression in A431 cells. ICAM‐I expression induced by either was not suppressed with cotreatment with IFN‐β. Furthermore, IFN‐β did not inhibit the translocation of protein kinase C (PKC) by TPA. It appears that the pathways involved in ICAM‐I expression induced by activation of PKC or increased in intracellular Ca+ are not affected by IFN‐β.
Cutaneous Leiomyosarcoma
Authors: Ikari Yuko , Tokuhashi Itaru , Haramoto Izumi , Morita Makoto , Chiba Toshiko , Shimoda Nagayoshi , Mizoguchi Masako , Seki Kenjiro , Tadokoro Mamoru , Ohya Kazumitsu 
Published: April 2015

AbstractA child's head‐sized tumor on the upper back developed in a 43‐year‐old man. In spite of an extensive operation for the tumor, he died of multiple metastasis 15 months after the operation. Histologically, tumor cells proliferated throughout the dermal and subcutaneous regions, and a boxed‐in appearance was noted with silver staining. Because electron microscopic observations strongly suggested a smooth muscle origin, we diagnosed this case as cutaneous and subcutaneous leiomyosarcoma.
Eruptive Pruritic Papular Porokeratosis
Authors: Kanzaki Tamotsu , Miwa Norimasa , Kobayashi Taisuke , Ogawa Shigeru 
Published: April 2015

AbstractThree cases of an unusual variant of porokeratosis (Mibelli) were described. Patients with disseminated superficial porokeratosis for some years suddenly developed intensively pruritic erythematous papules. Skin biopsies revealed that these papules contained cornoid lamellae on their tops. Pruritic papules subsided in several months, leaving slightly hyperkeratotic brown annular lesions which were shown to contain typical cornoid lamellae histopathologically. This type of porokeratosis has not been reported in the literature.
Primary Systemic Amyloidosis: An Unique Case Complaining of Diffuse Eyelid Swelling and Conjunctival Involvement
Authors: Iijima Shigeruko 
Published: April 2015

AbstractA 52‐year‐old Japanese woman with the chief complaint of marked swelling of her upper eyelids and a mass over the bulbar conjunctiva is reported. She previously noticed frequent purpura after minimal trauma, which was resolved shortly after taking some ascorbic acid. Laboratory data showed Ig‐G κ type M‐protein in the serum and κ type Bence‐Jones protein in the urine by Immunoelectrophoresis. Systemic examination showed mild hepatosplenomegaly, 1st degree of AV block, and a mild increase in plasma cells in the bone marrow biopsy. Histologically, the whole dermis of the eyelid skin and conjunctiva was replaced by a large quantity of amorphous, eosinophilic substances, which were diffusely positive with Direct Fast Scarlet 4BS. Immunohistochemical staining was positive for anti‐amyloid P component antibody. Typical amyloid fibrils were proved by electron microscopy. She was finally diagnosed as primary systemic amyloidosis with diffuse swollen eyelids and conjunctival mass, symptoms which in primary systemic amyloidosis are very rare.
An Association of Acanthosis Nigricans and Crouzon Syndrome
Authors: Koizumi Hiroko , Tomoyori Takako , Sato Kazuko C. , Ohkawara Akira 
Published: April 2015

AbstractAn 11‐year‐old Japanese female having acanthosis nigricans associated with Crouzon syndrome is reported. Crouzon syndrome is a craniostenotic craniofacial malformation associated with premature closure of selective calvarial sutures, exophthalmos, maxillary hypoplasia, and a beak‐shaped nose. It is an autosomal dominant inherited disorder. Crouzon syndrome is one of the syndromes which may be associated with acanthosis nigricans. The association of acanthosis nigricans with Crouzon syndrome is assumed to be a rare abnormality, although the true frequency is uncertain. We have reviewed the reported cases of acanthosis nigricans associated with Crouzon syndrome and characteristics were discussed.
Clinicopathological and Immunohistochemical Study of Basal Cell Carcinoma with Reference to the Features of Basement Membrane
Authors: Kirihara Yoshinobu , Haratake Joji , Horie Akio 
Published: April 2015

AbstractOne hundred and thirty‐five cases of basal cell carcinoma (BCC) were investigated, focusing upon factors determining a postoperative prognosis. Out of the 135, nine tumors on the face recurred. All of these nine tumors were insufficiently extirpated at the initial operation, and showed micronodular or infiltrative patterns with stromal fibrosis. Dividing the degree of dermal invasion into four levels, all tumors with recurrence reached levels 3 and 4, the two deeper groups. The immunohistochemical study using anti‐laminin and anti‐type IV collagen antibodies showed various changes of staining pattern around tumor cell nests, such as attenuation, disruption, and thickening of basement membrane, in contrast with the normal thinly continuous staining around nontumorous control epidermis. The disruption of basement membranes was remarkable around the tumor cells showing a micronodular growth pattern, although the discontinuity of basement membrane was observed in all types of BCCs to a greater or lesser degree. Ultrastructural thickening, multiplication, or discontinuity of basement membranes were found in all 19 cases examined with a greater or lesser degree, although they were most frequently observed around the cell nests showing micronodular growth patterns. It was concluded that deep dermal and marginal invasions were the most ominous signs of recurrence of BCCs. Although the disruption of basement membranes might participate in the local aggressiveness of BCCs, especially in the tumor cells showing micronodular infiltrative growth, other factors may concern the recurrence of BCCs.
A Case of Neutrophilic Dermatosis (ND) Complicated by Cryofibrinogenemia (CFGN) and Myelodysplastic Syndrome (MDS)
Authors: Watanabe Ryoji , Iijima Masafumi , Otsuka Fujio 
Published: April 2015

AbstractA case of neutrophilic dermatosis (ND) complicated by cryofibrinogenemia (CFGN) and myelodysplastic syndrome (MDS) is reported. Although the patient presented pancytopenia, the skin lesions were compatible with those of ND from the clinical and histopathological findings.Further, immunofluorescence technique revealed cryofibrinogen (CFG) deposits on the walls of the blood vessels in the skin lesion, and a high titer of CFG components was disclosed in the patient's peripheral blood. In this case, CFGN may have been associated with MDS; hence CFG deposits probably played a role, at least in part, in the skin lesion formation. This is probably the first case of ND complicated by CFGN. And careful examination of an underlying disease is recommended in association with ND and/or CFGN.
A Case of Congenital Trichofolliculoma
Authors: Ishii Norihisa , Kawaguchi Hiroshi , Takahashi Kazuo , Nakajima Hiroshi 
Published: April 2015

AbstractA case of congenital trichofolliculoma on the right cheek of a 3‐month‐old boy is reported. Trichofolliculoma usually appears in middle age; it is unusual in childhood. This is the first reported case of congenital trichofolliculoma.
Epidermal Changes in Active Vitiligo
Authors: Hann Seung Kyung , Park Yoon‐Kee , Lee Kwang‐Gil , Choi Eung Ho , Im Sungbin 
Published: April 2015

AbstractLight and electron‐microscopic studies were performed on the vitiligo and adjacent, normal appearing skin from 97 patients with actively spreading vitiligo and 19 patients with stable vitiligo. The vitiliginous skin revealed complete loss of pigment and melanocytes. In addition to degenerative changes in melanocytes, vacuolar changes of basal cells, epidermal infiltration of lymphocytes, dermal infiltration of lymphocytes, and melanophages in the upper dermis were also seen in the normal appearing skin adjacent to vitiliginous skin. These epidermal and dermal changes are more prominent in the skin of actively spreading vitiligo than in stable vitiligo. These findings suggest that the adjacent, normal appearing skin of actively spreading vitiligo shows some characteristic histopathologic findings, especially in the epidermis, indicating that cellular immunity could be involved in the pathogenesis of vitiligo.
Angiosarcoma of the Thigh with Thrombosis in the Femoral Artery
Authors: Miyamoto Torn , Mihara Motoyuki , Iwasaki Kazumi , Mishima Erika , Shimao Shuhei , Miyamoto Hisashi 
Published: April 2015

AbstractWe report a case of angiosarcoma with some peculiar clinical features developing on the left thigh of a 63‐year‐old man. The early primary lesion was erythematous with necrotic areas. The initial biopsy specimen of the lesion indicated a benign angioproliferative process. However, the necrotic area enlarged rapidly, ulcerated with severe pain, and thus was widely excised. The excised specimen had a malignant histologic appearance, particularly in the fascia, and was diagnosed as angiosarcoma. Following local recurrence, the lesion was complicated by thrombosis of the femoral artery at the affected site. A similar cutaneous lesion subsequently arose on the right lower leg, and thrombosis developed in the right femoral artery. Both legs had to be amputated. There were no distant metastases.
Reticulate Acropigmentation of Kitamura: A Case Report
Authors: Erel Arzu , Ozarmagan Guzin 
Published: April 2015

AbstractA Turkish female patient from Eastern Anatolia is described with the clinical and histopathological features of reticulate acropigmentation of Kitamura (RAK). The brother of the patient, who has similar clinical features, refuses to give a biopsy specimen. No similar lesions are noted on the other members of the patient's family. We have found the case worthwhile to report, since case reports on RAK from ethnic groups other than Japanese are still rare at present.
Melanocytic Neoplasia of the Sole: Diagnosis and Therapeutic Approach
Authors: Stefano Zurrida , Cesare Bartoli , Claudio Clemente , Fausto Chiesa , Aldo Bono , Natale Cascinelli 
Published: April 2015

AbstractWe examined retrospectively a series of 184 cases of melanocytic neoplasia of the sole observed and treated as out‐patients from January 1977 to December 1987, comparing clinical and histological diagnoses. The original clinical diagnoses were divided into nevi, pigmented lesions of suspected malignancy, cutaneous melanomas and others. Of the 170 cases diagnosed clinically as nevus none was of melanoma. The risk that a pigmented skin lesion diagnosed as clinically benign is melanoma is so low as not to constitute a clinical problem. It is concluded that systematic removal of sole nevi is unjustified. If, however, there is the smallest doubt concerning a sole lesion, it should be removed and examined histologically.
Levels of Serum IgE, Serum Soluble‐FcεRII, and FcεRII(+) Peripheral Blood Lymphocytes in Atopic Dermatitis
Authors: Kawai Keiichi , Kawai Kyozo , Kamei Nobuaki , Kishimoto Saburo 
Published: April 2015

AbstractLevels of serum IgE, serum soluble‐FcεRII (S‐FcεRII), and FcεRII(+) peripheral blood lymphocytes (PBL) were examined in 73 patients with atopic dermatitis (AD) and 17 control subjects with no atopic disease, in order to investigate the correlation of these parameters with AD. AD patients showed increases in IgE, S‐FcεRII and FcεRII(+)PBL as compared with control subjects. In AD patients, levels of serum IgE and FcεRII(+)PBL increased as the extent of dermatitis became more severe, while levels of serum S‐FcεRII showed no correlation with the extent of dermatitis. In 8 of the 73 AD patients who showed an improvement in their symptoms with treatment with topical corticosteroids or antihistamine, IgE, FcεRII(+)PBL, and S‐FcεRII were measured before and after treatment. FcεRII(+)PBL correlated with disease activity; IgE and S‐FcεRII did not show any such correlation.Patients with elevated IgE levels (IgE >5,000 U/ml) showed low levels of S‐FcεRII. Severely affected cases with a history of respiratory atopy also showed decreased S‐FcεRII levels. It is believed that S‐FcεRII binds to IgE in serum and may neutralize or down‐regulate IgE mediated allergic reactions. A low level of S‐FcεRII may cause an elevation of IgE and an exacerbation of the disease.
Optimization of Murine Keratinocyte Culture for the Production of Graftable Epidermal Sheets
Authors: Rouabhia Mahmoud , Germain Lucie , Bélanger François , Guignard Rina , Auger François A. 
Published: April 2015

AbstractThe aim of the present study was to optimize murine epidermal cell cultures in order to obtain graftable sheets. Newborn (1–3 days old) Balb/c mice skin were used to optimize culture media and plating cell concentration, then epidermal sheet production, and grafting.Epidermal cells were plated at various concentrations in different culture media containing low (0.1 mM) or high (>1 mM) Ca2+ levels. After a 3 day culture at the 104 cells/cm2 plating cell concentration, the percentage of differentiated cells was more than 80% in the high Ca2+ culture medium and less than 50% with bulky cells in the low Ca2+ culture medium. Under these conditions confluence was not obtained. At the 105 cells/cm2 seeding inoculum, the percentage of confluence increased to 95–100% during the first 72 h of culture in both high and low Ca2+ culture media. Three‐day‐old culture showed stratified multilayer epidermal sheets in the high calcium medium, and monolayer epidermal sheets were present in the low calcium medium after seeding keratinocytes in fibronectin precoated flasks. Seven days after plating, post confluent cultures were composed of a high percentage of differentiated cells (90%) with an increase in shedding cells in the medium.Considering the above morphological observations, sheets obtained with 105 cells/cm2 in MCDB‐153 (A), DME‐HAM (B) or GMEM (C) media after 3 days in culture were grafted. Twenty days after grafting, histological analysis of biopsies showed an epidermal structure and organization comparable to normal murine epidermis without hair follicles. Epidermal transplants showed a complete basement membrane, hemidesmosomes, and tonofilament bundles. Sheets obtained after seven day culture in all media showed lower coverage of the wound bed.These studies point out the importance of the plating cell and Ca2+ concentrations, and the culture time for murine keratinocyte confluence and differentiation to obtain graftable epidermal sheets.