bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analytic Evaluation of a Human ELISA Kit for Measurement of Inhibin B in Rat Samples
Authors: Coulson Michelle , Bickerton Sue , Betts Catherine J , Jacobsen Matt , Stewart Jane , Chapin Robert E. , Reagan William J. , Alvey James , Erdos Zoltan , Saldutti Louise Parks , Sonee Manisha , Bogdan Nancy , Singer Monica , Vinken Petra , Barlow Valerie , Czajkowski Karen , Kim James H. 
Published: January 2013

BACKGROUNDA cross‐laboratory analytic evaluation of a commercially available human inhibin B ELISA for measuring inhibin B in rat serum and plasma has been undertaken.METHODSDilution linearity, spiked recovery, intra‐ and inter‐assay precision, functional sensitivity, matrix effects, and frozen stability were assessed across five laboratories. Reference ranges were generated for male Sprague Dawley and Han Wistar rats.RESULTSAcceptable performance was defined as an overall assay coefficient of variation ≤ 20% with an intraday LLOQ ≤ 20 pg/ml. Intra‐ and inter‐assay precision and functional sensitivity (≤6.4 pg/ml) generally met these criteria, but with occasional evidence of greater variability, particularly at lower concentrations. Dilution linearity was acceptable with occasional low recovery. Acceptable recovery of kit calibrators from rat serum confirmed the absence of matrix effects. Matched serum and plasma samples gave comparable results. The signal increased on freezing, remained constant for ≥3 freeze‐thaw cycles and was generally stable for at least 8 weeks. Mean inhibin B ranged from 33.5 to 140.6 pg/ml in adult rats across laboratories, with some evidence for a decline from 6 to 9 weeks of age. Power calculations using preliminary reference range data indicated 10 animals/group would generally detect a 40% decrease in inhibin B at AstraZeneca, but laboratories with lower control values would require larger groups.CONCLUSIONSThe assay meets the analytical performance criteria; however, precision at the low end of the standard curve, biological variability, and low control values observed in some laboratories indicate that the utility of the assay may be limited in some laboratories.
Optimization and validation of spectrofluorimetric method for determination of cefadroxile and cefuroxime sodium in pharmaceutical formulations
Authors: Elbashir Abdalla A. , Ahmed Shazalia M. A. , Aboul‐Enein Hassan Y. 
Published: January 2013

ABSTRACTA simple, accurate, precise and validated spectrofluorimetric method is proposed for the determination of two cephalosporins, namely, cefadroxile (cefa) and cefuroxime sodium (cefu) in pharmaceutical formulations. The method is based on a reaction between cephalosporins with 1,2‐naphthoquinone‐4‐sulfonate in alkaline medium, to form fluorescent derivatives that are extracted with chloroform and subsequently measured at 610 and 605 nm after excitation at 470 and 460 nm for cefa and cefu respectively. The optimum experimental conditions have been studied. Beer's law is obeyed over the concentrations of 20–70 ng/mL and 15–40 ng/mL for cefa and cefu, respectively. The detection limits were 4.46 ng/mL and 3.02 ng/mL with a linear regression correlation coefficient of 0.9984 and 0.998, and recoveries ranging 97.50–109.96% and 95.73–98.89% for cefa and cefu, respectively. The effects of pH, temperature, reaction time, 1,2‐naphthoquinone‐4‐sulfonic concentration and extraction solvent on the determination of cefa and cefu, have been examined. The proposed method can be applied for the determination of cefa and cefu in pharmaceutical formulations in quality control laboratories. Copyright © 2013 John Wiley & Sons, Ltd.
Effect of liquid viscosity on hydrodynamics and bubble behaviour of an external‐loop airlift reactor
Authors: Wu Qian , Wang Xuekui , Wang Tiefeng , Han Mei , Sha Zuoliang , Wang Jinfu 
Published: January 2013

AbstractThe effect of liquid viscosity on hydrodynamics and bubble behaviour of an external‐loop airlift reactor within air–water system were studied by an electrical conductivity probe. Carboxyl methyl cellulose (CMC) was used to change liquid viscosity (1.00–51.25 cP). Gas holdup increased with superficial gas velocity increasing, as well as bubble Sauter diameter and bubble rise velocity. With liquid viscosity increasing, average gas holdup increased first then decreased, while bubble Sauter diameter was opposite, with critical viscosity µl ≈ 3.7 cP. The cross‐sectional average bubble Sauter diameter increased obviously after µl > 3.7 cP, and its size distribution was wider within the higher viscosity. Bubble rise velocity increased significantly and nearly unchanged after µl > 10.3 cP. Gas holdup first increased then remained unchanged with the axial height increasing. The radial profile of local gas holdup presented a central peak distribution, and the value at inlet was smaller than that at the higher position. Average gas holdup was correlated greatly with superficial gas velocity and liquid viscosity.
In vivo characterization of a bigenic fluorescent mouse model of Alzheimer's disease with neurodegeneration
Authors: Crowe Sarah E. , Ellis‐Davies Graham C.R. 
Published: January 2013

AbstractThe loss of cognitive function in Alzheimer's disease (AD) patients is strongly correlated with the loss of neurons in various regions of the brain. We have created a new fluorescent bigenic mouse model of AD by crossing “H‐line” yellow fluorescent protein (YFP) mice with the 5xFAD mouse model, which we call the 5XY mouse model. The 5xFAD mouse has been shown to have significant loss of L5 pyramidal neurons by 12 months of age. These neurons are transgenically labeled with YFP in the 5XY mouse, which enable longitudinal imaging of structural changes. In the 5XY mice, we observed an appearance of axonal dystrophies, with two distinct morphologies in the early stages of the disease progression. Simple swelling dystrophies are transient in nature and are not directly associated with amyloid plaques. Rosette dystrophies are more complex structures that remained stable throughout all imaging sessions, and always surrounded an amyloid plaque. Plaque growth was followed over 4 weeks, and significant growth was seen between weekly imaging sessions. In addition to axonal dystrophy appearance and plaque growth, we were able to follow spine stability in 4‐month old 5XY mice, which revealed no significant loss of spines. 5XY mice also showed a striking shrinkage of the neocortex at older ages (12–14 months). The 5XY mouse model may be a valuable tool for studying specific events in the degeneration of the neocortex, and may suggest new avenues for therapeutic intervention. J. Comp. Neurol. 521:2181–2194, 2013. © 2012 Wiley Periodicals, Inc.
Digital curation in the academic library job market
Authors: Kim Jeonghyun , Warga Edward , Moen William 
Published: January 2013

AbstractWith the increasingly important role librarians play in the fast‐paced and data‐intensive digital curation environment, there is a need to identify the qualifications and responsibilities expected by employers. An investigation of 110 recent job advertisements was conducted to identify competencies required of individuals working in the digital curation field. The job ads analysis serves as an important indicator of the emerging requirements for a qualified workforce in the field of digital curation in the academic library job market.
Two‐staged hindfoot reconstruction with vascularized fibula graft for calcaneal osteomyelitis caused by methicillin‐resistant Staphylococcus aureus: A case report
Authors: Endo Jun , Kuniyoshi Kazuki , Mochizuki Makondo , Shimoyama Katsuhito , Koyama Tadaaki , Aiba Atsuomi , Kadota Ryo , Sasaki Yasuhito 
Published: January 2013

AbstractHindfoot reconstruction after calcaneal osteomyelitis is a challenging procedure designed to restore the weight bearing function of the heel and to allow a functional reconstruction of the Achilles tendon. Some patients require subtalar arthrodesis after primary calcaneal osteosyntesis or hindfoot reconstruction due to the considerable pain associated with weight‐bearing caused by the irregular surface of the subtalar joint. To date, no reports have shown a case of hindfoot reconstruction with subtalar arthrodesis using a pedicled vascularized fibula graft. We report a case of a 24‐year‐old woman who presented with calcaneal methicillin‐resistant Staphylococcus aureus osteomyelitis after open comminuted fracture due to a fall. Radical debridement of bone and soft tissue was repeated six times in combination with negative pressure wound therapy, followed by hindfoot reconstruction with pedicled vascularized fibula and subtalar arthrodesis. Good functional restoration had been achieved by the final follow‐up 18 months after surgery. © 2013 Wiley Periodicals, Inc. Microsurgery, 2013.
Advantage of tapered and graded AlGaN electron blocking layer in InGaN‐based blue laser diodes
Authors: Yang Wei , Li Ding , He Juan , Hu Xiaodong 
Published: January 2013

AbstractWe investigate the advantage of tapered and graded AlGaN electron blocking layer (EBL) in InGaN‐based blue laser diodes (LDs) theoretically by self‐consistent Schrödinger‐Poisson method together with transfer matrix method. Such optimized EBLs was found to suppress electron leakage current as well as enhance hole injection compared to their conventional counterpart. More uniform carrier distribution and thus uniform local gain profile is obtained in the active region of LDs with optimized EBLs. Slightly enhancement of optical confinement factor is also obtained. As a result, LDs with new EBL structure demonstrate better device performance with decrease of threshold current density and increase of light output power (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Extended Defects in Semiconductors
Authors: Komninou Philomela 
Published: January 2013

AbstractThis Proceedings issue of physica status solidi contains papers presented as oral and poster contributions at the International Conference on Extended Defects in Semiconductors (EDS 2012). EDS 2012 belonged to the biennial Extended Defects in Semiconductors conference series that started in 1978 with a meeting in Hünfeld, Germany. Subsequent meetings took place in Poland, France, Great Britain, Germany, Russia, and Italy. EDS 2010 was held at the University of Sussex in Brighton, UK.EDS 2012 took place in Thessaloniki, Greece, on June 24‐29, 2012. It was organized by the Department of Physics of the Aristotle University of Thessaloniki.The aim of the conference was to target the overall vision of research activities of the scientific community, based on needs that stem from technological bottlenecks involving structural defects in present day and future polynanocrystalline and nanostructured semiconductor material systems and their role in the functionality of innovative nanodevices. Within this objective, invited talks and contributions provided a stimulating conference program covering the following topics:– Thin films and heterostructures– Low‐dimensional systems and related defects: semiconductor nanocrystals, quantum wells, quantum dots, nanowires– Interface structures and defect interactions– Doping/irradiation/implantation‐induced defects– Electronic structure of defects– Defect engineering, strain engineering– Mechanical properties and dislocation dynamics– Role of defects in opto‐electronic and magnetic properties– Degradation mechanisms in semiconductor devices– Atomistic and multiscale simulations, modeling approaches of defects, interfaces and nanostructures– Advanced characterization techniques and methods for the investigation of semiconductor materialsI would like to thank the members of the EDS 2012 International Advisory, Program and Organizing Committees, as well as the authors, referees, and guest editors, for their invaluable contributions towards the success of this conference and proceedings (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
3‐Methylglutaconic aciduria—lessons from 50 genes and 977 patients
Authors: Wortmann Saskia B. , Kluijtmans Leo A. J. , Rodenburg Richard J. , Sass Jörn Oliver , Nouws Jessica , van Kaauwen Edwin P. , Kleefstra Tjitske , Tranebjaerg Lisbeth , de Vries Maaike C. , Isohanni Pirjo , Walter Katharina , Alkuraya Fowzan S. , Smuts Izelle , Reinecke Carolus J. , van der Westhuizen Francois H. , Thorburn David , Smeitink Jan A. M. , Morava Eva , Wevers Ron A. 
Published: January 2013

AbstractElevated urinary excretion of 3‐methylglutaconic acid is considered rare in patients suspected of a metabolic disorder. In 3‐methylglutaconyl‐CoA hydratase deficiency (mutations in AUH), it derives from leucine degradation. In all other disorders with 3‐methylglutaconic aciduria the origin is unknown, yet mitochondrial dysfunction is thought to be the common denominator. We investigate the biochemical, clinical and genetic data of 388 patients referred to our centre under suspicion of a metabolic disorder showing 3‐methylglutaconic aciduria in routine metabolic screening. Furthermore, we investigate 591 patients with 50 different, genetically proven, mitochondrial disorders for the presence of 3‐methylglutaconic aciduria. Three percent of all urine samples of the patients referred showed 3‐methylglutaconic aciduria, often in correlation with disorders not reported earlier in association with 3‐methylglutaconic aciduria (e.g. organic acidurias, urea cycle disorders, haematological and neuromuscular disorders). In the patient cohort with genetically proven mitochondrial disorders 11 % presented 3‐methylglutaconic aciduria. It was more frequently seen in ATPase related disorders, with mitochondrial DNA depletion or deletion, but not in patients with single respiratory chain complex deficiencies. Besides, it was a consistent feature of patients with mutations in TAZ, SERAC1, OPA3, DNAJC19 and TMEM70 accounting for mitochondrial membrane related pathology. 3‐methylglutaconic aciduria is found quite frequently in patients suspected of a metabolic disorder, and mitochondrial dysfunction is indeed a common denominator. It is only a discriminative feature of patients with mutations in AUH, TAZ, SERAC1, OPA3, DNAJC19TMEM70. These conditions should therefore be referred to as inborn errors of metabolism with 3‐methylglutaconic aciduria as discriminative feature.
Corporate propping through related‐party transactions
Authors: Williams Maggie P. , Taylor Dennis W. 
Published: January 2013

PurposeThe purpose of this paper is to investigate the phenomenon in China of listed companies propping up their reported earnings through the use of abnormal related‐party sales. It is hypothesised that two factors associated with securities regulation of listed companies in China will distort the market for ownership control and consequently impact on the practice of propping. The first factor is the firm's risk of being classified as a “special treatment” firm and potentially being delisted. The second factor is the proportion of non‐tradable shares retained by a State‐based controlling shareholder from a government allocation.Design/methodology/approachThe hypotheses are modelled and tested using secondary data from 2010 annual reports and a financial database for companies sampled from the top 100 on the Shanghai and Shenzen Stock Exchanges.FindingsBoth hypotheses are supported. Abnormal sales (a proxy for propping) are found to be higher for firms whose ROE had fallen to a level that potentially put them under “special treatment” scrutiny, and also are higher for firms whose proportion of non‐tradeable shares had declined.Originality/valuePrior studies on propping have focused on companies faced with moderate financial shock being propped up by controlling shareholders so as to preserve their future opportunities to tunnel funds away from minority shareholders. Not previously investigated are the potential side effects of securities regulations on controlling shareholders' incentive for propping, namely, the identification that propping relates to the level of ROE needed to avoid “special treatment” status and the proportion of non‐tradable shares needed as a buffer in the market for corporate control.
Exploring the nature and effects of perception and image in hospitality and tourism
Authors: Ingram Hadyn , Grieve Deborah 
Published: January 2013

PurposeThe purpose of this paper is to provide a general exploratory review of perception and image in hospitality and tourism. The paper considers the nature of perception and image and how they can affect buying behaviour for hospitality and tourism products.Design/methodology/approachThe authors use the literature to explore the nature of perception and image and how these can be manifested. A model is elucidated which might help to explain purchase decision making for hospitality and tourism products and services.FindingsFindings supplement the authors' views about the importance of this issue for managing and marketing hospitality and tourism products more effectively. The model seeks to make explicit the stages in the process from image formation to purchase.Practical implicationsAs both authors are working practitioners, this paper offers a model and a practical approach which will form the basis for a practitioner panel, from which the model will be refined.Originality/valueThere has been little research in an area which seeks to integrate image formation with practical implications for hospitality and tourism organizations.
Research on complex product cost estimation based on the N‐GM (0, N) model
Authors: Hongzhuan Chen , Kaifeng Fan , Zhigeng Fang 
Published: January 2013

PurposeThe purpose of this paper is to propose a prediction model to predict the cost of complex products with lack of data. The cost estimating is one of the key elements of arguments around technological economy and investment decision‐making process of complex product.Design/methodology/approachA complex product has many characteristics, such as complex structure, large investment, high risk and it usually falls into small‐batch‐production category. Its cost estimation samples are small and cost data are very limited. Based on the characteristics of complex product and cost estimating, this paper introduces performance parameters sequence of associated known data, establishes an N‐GM (0, N) model of characteristic sequence with straddle missing data.FindingsOn the basis of the known key performance parameter sequence, N‐GM (0, N) model is used to predict the grey interval of overall cost vacancy data. Overall cost vacancy data is whitened by sorting reference sequence and realizing complex product overall cost estimation.Practical implicationsThe method introduced in the paper can be used to solve practical problems, especially cost prediction of complex products with poor data. The model is also applied on the overall cost and the key component cost estimation of similar but different complex products. Moreover, it provides potential theoretical support for the development of complex product industry in the future.Originality/valueIn this paper, the complex product, which now plays a strategic industrial role in China, is systematically studied by utilizing a new methodology based on grey systems, especially the cost evaluation of the complex product. The use of grey correlation analysis in screening control key item index of complex product cost, the overall cost sequence of the complex product as related sequence and sorting reference sequence, the paper predicts and whitens vacant key item index, obtaining the key item cost index of complex product.
Pitfalls in CT diagnosis of appendicitis: Pictorial essay
Authors: Shademan Ashkan , Tappouni Rafel FR 
Published: January 2013

SummaryDespite the high diagnostic accuracy of CT for appendicitis, numerous pitfalls exist that may result in a misdiagnosis. This pictorial review outlines the potential pitfalls in the CT diagnosis of appendicitis that includes atypical position of the appendix and coexisting pathologies. Various mimickers of appendicitis and clinical dilemmas will be highlighted. Upon completion, the reviewer should have an improved ability to recognise appendicitis mimickers and identify equivocal or atypical findings.
A RING‐type E3 ligase controls anther dehiscence by activating the jasmonate biosynthetic pathway gene DEFECTIVE IN ANTHER DEHISCENCE1 in Arabidopsis
Authors: Peng Yan‐Jhu , Shih Ching‐Fang , Yang Jun‐Yi , Tan Choon‐Meng , Hsu Wei‐Han , Huang Yu‐Pei , Liao Pei‐Chun , Yang Chang‐Hsien 
Published: January 2013

SummarySuppression of expression of DAF [DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1)‐Activating Factor], a gene that encodes a putative RING‐finger E3 ligase protein, causes non‐dehiscence of the anthers, alters pollen development and causes sterility in 35S:DAF RNAi/antisense Arabidopsis plants. This mutant phenotype correlates with the suppression of DAF but not with expression of the two most closely related genes, DAFL1/2. The expression of DAD1 was significantly reduced in 35S:DAF RNAi/antisense plants, and complementation with 35S:DAF did not rescue the dad1 mutant, indicating that DAF acts upstream of DAD1 in jasmonic acid biosynthesis. This assumption is supported by the finding that 35S:DAF RNAi/antisense plants showed a similar cellular basis for anther dehiscence to that found in dad1 mutants, and that external application of jasmonic acid rescued the anther non‐dehiscence and pollen defects in 35S:DAF antisense flowers. We further demonstrate that DAF is an E3 ubiquitin ligase and that its activity is abolished by C132S and H137Y mutations in its RING motif. Furthermore, ectopic expression of the dominant‐negative C132S or H137Y mutations causes similar indehiscence of anthers and reduction in DAD1 expression in transgenic Arabidopsis. This result not only confirms that DAF controls anther dehiscence by positively regulating the expression of DAD1 in the jasmonic acid biosynthesis pathway, but also supports the notion that DAF functions as an E3 ubiquitin ligase, and that the conserved RING‐finger region is required for its activity.
Routine bacterial screening of apheresis platelets on Day 4 using a rapid test: a 4‐year single‐center experience
Authors: Dunbar Nancy M. , Kreuter Justin D. , Marx‐Wood Cynthia R. , Dumont Larry J. , Szczepiorkowski Zbigniew M. 
Published: January 2013

BackgroundThe platelet (PLT) Pan Genera Detection test (PGD) is a rapid bacterial detection system used to screen PLTs for bacterial contamination. We report a single center 46‐month experience with secondary screening of apheresis PLTs by PGD testing.Study Design and MethodsExisting testing records of apheresis PLTs screened by PGD from July 2008 to April 2012 were reviewed. All PLT units were initially screened by routine postcollection culture methods. Secondary screening using PGD was performed for indated PLTs on PLT storage Day 4 and for outdated PLTs on Day 8.ResultsA total of 8535 apheresis PLTs were available in inventory during the study period. Of these, 5030 (58.9%) were dispensed and transfused before PGD testing and 3505 (41.1%) underwent PGD testing on Day 4. Twenty‐five units tested on Day 4 were PGD initial reactive (0.71%). All were confirmed to be false positive by repeat PGD testing in triplicate (n = 20) or by confirmatory culture (n = 5). An additional 364 units that were PGD nonreactive on Day 4 were approved for transfusion on Day 6 or Day 7 due to urgent clinical need. A total of 371 outdated units underwent repeat PGD testing before discard on Day 8; all were nonreactive.ConclusionSecondary PGD testing of culture‐screened apheresis PLTs results in low yield in a medium‐sized transfusion service. Use of PGD testing on Day 4 may allow for extension of the apheresis PLT shelf life to Day 7 for hospitals that face supply constraints.