bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms of regulation of glycolipid metabolism by natural compounds in plants: effects on short-chain fatty acids
Authors: Li Jiarui , Zhao Jinyue , Tian Chuanxi , Dong Lishuo , Kang Zezheng , Wang Jingshuo , Zhao Shuang , Li Min , Tong Xiaolin 
Published: July 2024

Abstract
Background
Natural compounds can positively impact health, and various studies suggest that they regulate glucose‒lipid metabolism by influencing short-chain fatty acids (SCFAs). This metabolism is key to maintaining energy balance and normal physiological functions in the body. This review explores how SCFAs regulate glucose and lipid metabolism and the natural compounds that can modulate these processes through SCFAs. This provides a healthier approach to treating glucose and lipid metabolism disorders in the future.

Methods
This article reviews relevant literature on SCFAs and glycolipid metabolism from PubMed and the Web of Science Core Collection (WoSCC). It also highlights a range of natural compounds, including polysaccharides, anthocyanins, quercetins, resveratrols, carotenoids, and betaines, that can regulate glycolipid metabolism through modulation of the SCFA pathway.

Results
Natural compounds enrich SCFA-producing bacteria, inhibit harmful bacteria, and regulate operational taxonomic unit (OTU) abundance and the intestinal transport rate in the gut microbiota to affect SCFA content in the intestine. However, most studies have been conducted in animals, lack clinical trials, and involve fewer natural compounds that target SCFAs. More research is needed to support the conclusions and to develop healthier interventions.

Conclusions
SCFAs are crucial for human health and are produced mainly by the gut microbiota via dietary fiber fermentation. Eating foods rich in natural compounds, including fruits, vegetables, tea, and coarse fiber foods, can hinder harmful intestinal bacterial growth and promote beneficial bacterial proliferation, thus increasing SCFA levels and regulating glucose and lipid metabolism. By investigating how these compounds impact glycolipid metabolism via the SCFA pathway, novel insights and directions for treating glucolipid metabolism disorders can be provided.
Aprotinin in high-risk isolated coronary artery bypass graft patients: a 3-year propensity matched study
Authors: Makam Rishab , Balaji Ayush , Al Munaer Marwan , Bajaj Shantanu , Hussein Nabil , Loubani Mahmoud 
Published: July 2024

Abstract
Background
Aprotinin, a serine protease inhibitor, has been used variably in cardiac surgery amidst ongoing debates about its safety following several previous studies. This study assesses the outcomes of aprotinin in high-risk isolated Coronary Artery Bypass Graft (iCABG) patients.

Methods
The study retrospectively analysed a cohort of 1026 iCABG patients, including 51 patients who underwent aprotinin treatment. Logistic regression powered score matching was employed to compare aprotinin patients with a control group, in a propensity-matched cohort of 96 patients. The primary outcome measured was in-hospital death, with secondary outcomes including renal dysfunction, stroke, myocardial infarction, re-exploration for bleeding or tamponade, and postoperative stay durations.

Results
The aprotinin cohort had high-risk preoperative patients with significantly higher EUROSCORE II values, 7.5 (± 4.2), compared to 3.9 (± 2.5) in control group. However, aprotinin group showed no statistically significant increase (p-value: 0.44) in hospital mortality with OR 2.5 [95% CI 0.51, 12.3]. Major secondary outcome rates of renal replacement therapy and postoperative stroke compared to the control group were also statistically insignificant between the two groups.

Conclusion
This study suggests that aprotinin may be safely used in a select group of high-risk iCABG patients. The reintroduction of aprotinin under specific conditions reflects its potential benefits in managing bleeding in high-risk surgeries, but also underscores the complexity of its risk-benefit profile in such critical care settings. Nonetheless, it highlights the importance of carefully selecting patients and conducting additional research, including larger and more controlled studies to fully comprehend the potential risks and benefits of aprotinin.
The burden and its determinants of mental health distress among adolescents dwelling in Africa: a systematic review and meta-analysis
Authors: Tinsae Techilo , Shumet Shegaye , Takelle Girmaw Medfu , Rtbey Gidey , Melkam Mamaru , Andualem Fantahun , Nakie Girum , Segon Tesfaye , Koye Selam , Fentahun Setegn , Alemu Wondale Getinet , Tadesse Gebresilassie 
Published: July 2024

Abstract
Background
Adolescent mental health issues are emerging as a significant public health concern across many low- and middle-income countries, particularly in Africa. This study aims to evaluate the aggregated prevalence and contributing risk factors of mental health distress among adolescents in Africa.

Methodology
A comprehensive search of PubMed, PsycINFO, Web of Science, Google Scholar, and HINARI databases was conducted to identify relevant articles on the prevalence and risk factors associated with mental health distress among African adolescents, published up to December 2023. The quality of the selected studies was assessed using the Newcastle-Ottawa Quality Assessment Scale. Heterogeneity among the studies was evaluated using the I² statistical test. Potential publication bias was assessed through a funnel plot and Egger’s statistical test. This systematic review was registered with PROSPERO under reference number CRD42023494665.

Results
Eighteen studies encompassing data from 37,016 adolescents were included in the analysis. The overall prevalence of mental health distress among adolescents in Africa was found to be 27.34% (95% CI: 23.18–31.50). The occurrence of mental health distress is observed in older adolescents at a prevalence of 29.44% (95% CI: 23.26–35.66) and in younger adolescents at 24.73% (95% CI: 11.96–37.51). Significant risk factors identified included bullying victimization, with an odds ratio (POR) of 1.30 (95% CI: 1.16, 1.46), and experiencing hunger, with an odds ratio (POR) of 2.10 (95% CI: 1.13, 3.91).

Conclusion
The findings indicate a high prevalence of mental health distress among adolescents in Africa, highlighting the widespread impact on this demographic. These results underscore the urgent need for targeted interventions to prevent and address mental health distress among adolescents. Further research on a global scale is essential to develop effective prevention and treatment strategies tailored to this age group.
Effects of storage conditions on the stability of qPCR reagents: implications for environmental DNA detection
Authors: Lopez Mark Louie D. , Crichton Ellika M. , Allison Michael J. , Dema Anna H. , Bonderud Matthew T. , Helbing Caren C. 
Published: July 2024

Abstract
Objective
Environmental DNA (eDNA) detection is a transformative tool for ecological surveys which in many cases offers greater accuracy and cost-effectiveness for tracking low-density, cryptic species compared to conventional methods. For the use of targeted quantitative PCR (qPCR)-based eDNA detection, protocols typically require freshly prepared reagents for each sample, necessitating systematic evaluation of reagent stability within the functional context of eDNA standard curve preparation and environmental sample evaluation. Herein, we assessed the effects of long-term storage and freeze–thaw cycles on qPCR reagents for eDNA analysis across six assays.

Results
Results demonstrate qPCR plates (containing pre-made PCR mix, primer-probe, and DNA template) remain stable at 4 °C for three days before thermocycling without fidelity loss irrespective of qPCR assay used. Primer-probe mixes remain stable for five months of − 20 °C storage with monthly freeze-thaw cycles also irrespective of qPCR assay used. Synthetic DNA stocks maintain consistency in standard curves and sensitivity for three months under the same conditions. These findings enhance our comprehension of qPCR reagent stability, facilitating streamlined eDNA workflows by minimizing repetitive reagent preparations.
Proposal of a method to analyze children’s flood risk exposure and risk perception using GPS tracking data and questionnaire survey
Authors: Sakamoto Jun , Laudari Suresh , Fujioka Masaki , Hara Tadashi 
Published: July 2024

Abstract
Background
Climate change has made the relationship between disaster and sustainable development more critical. Especially in developing countries, disasters frequently devastate local communities and hinder progress. Children are at a higher risk during catastrophes; however, their knowledge of disaster risk is limited. Education for disaster risk reduction can bring the necessary changes for a safe society. School disaster prevention education can raise awareness through lectures and games, but interactive dialogues between students and teachers can be more effective. Also, GPS tracking can be a valuable tool for understanding people’s behavior during disasters.

Methodology
This study proposes a method to analyze the relationship between students’ daily behavior and their experiences of flood damage and preparedness for disaster risk. It is a simple method using questionnaires and GPS loggers and can be applied in mountainous areas in developing countries where equipment is inadequate. The proposed method will be used to understand the current state of disaster risk among children and identify disaster risk reduction issues. The study area lies in Paluntar Municipality, Gorkha district, Nepal. Forty-five students from two schools in the region participated in the study.

Results
The results showed that students who commute to school in areas inundated by heavy rainfall in recent years were not adequately prepared for disasters, even though they had experienced flood damage in the past and were significantly more concerned about future harm. Our field observations and interviews of teacher-student relationships showed that students were very attentive to their teachers’ instruction. Teachers understand the dangers of heavy rainfall and how to prepare for it, but students need to learn how to deal with it. In other words, the school staff’s disaster education to the students effectively reduces disasters. Early warning is needed when severe floods are expected during the rainy season.
Endogenous Estrogens and Brain Activation During Verbal Memory Encoding and Recognition in the Postmenopause
Authors: Schroeder Rachel A , Thurston Rebecca C , Wu Minjie , Aizenstein Howard J , Derby Carol A , Maki Pauline M 
Published: July 2024

Abstract

Context
Changes in verbal memory have been reliably reported across the menopause transition. To understand the role of endogenous estrogens in verbal memory performance, this study assessed the associations of endogenous estradiol and estrone with brain network connectivity during a verbal memory fMRI task.

Objective
Determine associations of endogenous estrogens with memory systems in the postmenopausal brain and evaluate clinical significance.

Methods
In the MsBrain cohort (n = 199, mean age 59.3 ± 3.9 years, 83.9% White), we examined the cross-sectional association of serum estradiol (E2) and estrone (E1), measured using liquid chromatography–tandem mass spectrometry (LC-MS/MS), during a functional magnetic resonance imaging (fMRI) task of word encoding and recognition. To characterize the clinical significance of those associations, we examined the magnitude of activation in relation to a neuropsychological measures of memory and affect.

Results
Endogenous E2 was positively associated with activation in temporal and frontal cortices during encoding and negatively associated with one prefrontal region during recognition (P &lt; .05). Activation in the left inferior frontal gyrus was associated with memory performance (β [SE] = 0.004 [0.002]; P &lt; .05), and anxiety (β [SE] = −0.100 [0.050]; P &lt; .05). The left middle frontal gyrus was associated with memory performance (β [SE] = 0.006 [0.002]; P &lt; .01), depression, and anxiety. The left superior temporal gyrus (STG) was associated with depression (β [SE] = −0.083 [0.036]; P &lt; .05) and anxiety (β [SE] = −0.134 [0.058]; P &lt; .05). E1 was positively associated with activation in a range of brain areas including bilateral STG and right superior frontal gyrus during encoding (P &lt; .05). Activation of the left insula and precentral gyrus were associated with symptoms of depression and anxiety. None related to memory.

Conclusion
The function of brain areas critical to memory performance varies with estrogen levels in the postmenopause, even though those levels are low. Higher levels of E2 may facilitate memory performance through enhanced function of temporal and frontal cortices during encoding of verbal material.
Randomised controlled trials in vascular access devices in emergency departments: a scoping review protocol
Authors: Xu Hui (Grace) , Duff Jed , Marsh Nicole 
Published: July 2024

Background: The significance of vascular access devices for patients in the emergency department (ED) is undeniable. When it comes to evaluating the effectiveness of interventions, randomised controlled trials (RCTs) stand out as the most reliable sources of evidence compared with other study designs. Aim: To explore and synthesise the findings from RCTs related to vascular access devices in the ED setting. Methods: A systematic search will be conducted in electronic medical databases including the Cochrane Central Register of Controlled Trials, Pubmed, CINAHL and Embase databases. All RCTs focusing on peripheral intravenous catheters, central venous catheters and intraosseous catheters, published in English and Chinese in peer-reviewed journals within the past decade, will be included. Conclusion: This scoping review will summarise the current state of evidence for vascular access devices in the ED setting. This will identify gaps in the literature and, in turn, assist clinicians and researchers in pinpointing areas for future exploration and provide a valuable guide for future research.
Tibial insert design significantly alters knee kinematics using a single cruciate-retaining total knee implant
Authors: Faschingbauer Martin , Hambrecht Jakob , Schwer Jonas , Martin John R. , Reichel Heiko , Seitz Andreas 
Published: July 2024

AimsPatient dissatisfaction is not uncommon following primary total knee arthroplasty. One proposed method to alleviate this is by improving knee kinematics. Therefore, we aimed to answer the following research question: are there significant differences in knee kinematics based on the design of the tibial insert (cruciate-retaining (CR), ultra-congruent (UC), or medial congruent (MC))?MethodsOverall, 15 cadaveric knee joints were examined with a CR implant with three different tibial inserts (CR, UC, and MC) using an established knee joint simulator. The effects on coronal alignment, medial and lateral femoral roll back, femorotibial rotation, bony rotations (femur, tibia, and patella), and patellofemoral length ratios were determined.ResultsNo statistically significant differences were found regarding coronal alignment (p = 0.087 to p = 0.832). The medial congruent insert demonstrated restricted femoral roll back (mean medial 37.57 mm; lateral 36.34 mm), while the CR insert demonstrated the greatest roll back (medial 42.21 mm; lateral 37.88 mm; p < 0.001, respectively). Femorotibial rotation was greatest with the CR insert with 2.45° (SD 4.75°), then the UC insert with 1.31° (SD 4.15°; p < 0.001), and lowest with the medial congruent insert with 0.8° (SD 4.24°; p < 0.001). The most pronounced patella shift, but lowest patellar rotation, was noted with the CR insert.ConclusionThe MC insert demonstrated the highest level of constraint of these inserts. Femoral roll back, femorotibial rotation, and single bony rotations were lowest with the MC insert. The patella showed less shifting with the MC insert, but there was significantly increased rotation. While the medial congruent insert was found to have highest constraint, it remains uncertain if this implant recreates native knee kinematics or if this will result in improved patient satisfaction.Cite this article: Bone Jt Open 2024;5(7):592–600.
“Sickness has no time”: Awareness and perceptions of health care workers on universal health coverage in Uganda
Authors: Ifeagwu Susan C. , Nakaboga Kikonyogo Ruth , Nakkazi Suzan , Beinomugisha Joshua , Ojiambo Wandera Stephen , Kiwanuka Suzanne N. , King Rachel , Van Bortel Tine , Brayne Carol , Parkes-Ratanshi Rosalind 
Published: July 2024

Introduction
Each person having access to needed health services, of sufficient quality, and without suffering financial hardship, defined as universal health coverage (UHC) by the World Health Organization, is critical to improve population health, particularly for vulnerable populations. UHC requires multisectoral collaboration and good governance, and this will require buy-in of key stakeholders; but their views are under-documented. The aim of this stakeholder analysis was to explore the awareness and perceptions of UHC by health care workers (HCWs) in Uganda.

Methods
A mixed-methods study was conducted based on primary data from HCWs including an online QualtricsXM survey of 274 HCWs (from a database of persons who had received training at an academic institution), 23 key informant semi-structured interviews, and one eight-person focus group discussion. Data was collected from February to April 2022. Microsoft Excel and R Programme were used for quantitative analyses and NVivo version 12 for qualitative analyses.

Results
HCWs attributed a high level of importance to UHC in Uganda. Participants discussed national communication and management practices, organisational roles, health financing and power dynamics, health care demand and the impact of and learnings from COVID-19. Four main themes–each with related sub-themes–emerged from the interview data providing insights into: (1) communication, (2) organisation, (3) power, and (4) trust.

Conclusion
There is a critical need for better communication of UHC targets by policymakers to improve understanding at a grassroots level. Results indicated that ensuring trust among the population through transparency in metrics and budgets, strong accountability measures, awareness of local cultural sensitivities, sensitisation of the UHC concept and community inclusion will be essential for a multisectoral roll out of UHC. Further provision of quality health services, a harmonisation of efforts, increased domestic health financing and investment of HCWs through fair remuneration will need to underpin the delivery of UHC.
Utility of a bone health clinic in bridging the osteoporosis care gap: Prescribing habit review at an academic institution
Authors: Riley Marisa , Crossman Derek , Kocis Paul , Hassenbein Susan , Fox Edward 
Published: July 2024

Objective
To analyze osteoporosis medication prescribing trends across specialties in the context of a Bone Health Clinic.

Introduction
Osteoporosis affects over 10 million adults in the US, taking a significant toll on patients and the healthcare system. Although screening methods and treatments are improving, the disease remains underdiagnosed and undertreated. This study aims to evaluate the prescribing trends of osteoporosis medication among department specialties to delineate the benefits of a bone health clinic.

Methods
Retrospective data collection identified and analyzed patients within the Penn State Health system prescribed one of the following osteoporosis medications: Bisphosphonate, denosumab, romosozumab, teriparatide, abaloparatide, or raloxifene. Date range: 4/18/2016 to 4/14/2021. Data collection identified the specialty origin of prescriptions for osteoporosis medications across various medical specialties (e.g., orthopaedics, family medicine, and internal medicine).

Results
10,736 prescription orders were issued to patients with an average age of 68 years. Non-Hispanic Caucasian patients received 88.6% of prescriptions, followed by Asian (3.4%) and African American (2.2%). Female patients accounted for 87.8% of all prescriptions. The Bone Health Clinic under two orthopaedic providers wrote 3,619 prescriptions, averaging 361.9 prescriptions per provider per year—marking the highest rate among specialties. The clinic prescriptions constituted 33.7% of all prescriptions across specialties. Orthopaedic surgery prescribed the most denosumab, romosozumab, teriparatide, and abaloparatide prescriptions, and had the highest number of male osteoporosis patients compared to other specialties (15.6%), consequently prescribing the most male prescriptions (578).

Conclusion
Establishing a bone health clinic dedicated to osteoporosis management leads to significantly higher prescription rates per provider, increased utilization of anabolic therapies compared to other specialties, and more male patients being treated—an often-neglected population in osteoporosis.
Efficacy of high-intensity interval training versus continuous training on serum myonectin and lipid outcomes in adults with metabolic syndrome: A post-hoc analysis of a clinical trial
Authors: Petro Jorge L. , Fragozo-Ramos María Carolina , Milán Andrés F. , Aristizabal Juan C. , Calderón Juan C. , Gallo-Villegas Jaime 
Published: July 2024

Background
Myonectin is a myokine with potential effects on the lipid metabolism; however, its regulation by exercise in humans remains unclear. We aimed to compare the efficacy of high-intensity interval training low-volume (HIIT) versus moderate-intensity continuous training (MICT) on serum myonectin, serum lipids, appendicular fat and lean mass, and intramuscular lipids in humans.

Methods
Secondary analysis of a controlled, randomized, clinical trial in adults of both sexes with metabolic syndrome, who underwent a supervised, three-times/week, 12-week treadmill program. HIIT (n = 29) consisted of six intervals with one-minute, high-intensity phases at 90% of peak oxygen consumption (VO2peak) for a total of 22 min. MICT (n = 31) trained at 60% of VO2peak for 36 min. Serum myonectin was measured using a human enzyme-linked immunosorbent assay. Lipid profile was determined by enzymatic methods and free fatty acids (FFA) were measured by gas chromatography. Fat and lean mass were assessed by dual-energy X-ray absorptiometry. Intramuscular lipids were measured through proton magnetic resonance spectroscopy.

Results
Subjects had a mean age of 50.8±6.0 years and body mass index of 30.6±4.0 kg/m2. Compared to MICT, HIIT was not superior at increasing serum myonectin (p = 0.661) or linoleic acid (p = 0.263), reducing palmitic (p = 0.286) or stearic acid (p = 0.350), or improving lipid profile (all p>0.05), appendicular fat mass index ―AFMI― (p = 0.713) or appendicular lean mass percentage ―ALM― (p = 0.810). Compared to baseline, only HIIT significantly increased myonectin (p = 0.042), with a large effect size, although both interventions reduced AFMI and increased ALM with a large effect size. Lipid profile, FFA and intramuscular lipids did not change in any intervention group (p>0.05).

Conclusions
Compared to MICT, HIIT low volume did not demonstrate superiority in improving serum lipids. The fact that both training types reduced AFMI without paralleled significant changes in serum myonectin suggests that this myokine may have a minor effect on short-middle-term exercise-induced fat mobilization.