bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends in Toxic Alcohol Exposures in the United States from 2000 to 2013: A Focus on the Use of Antidotes and Extracorporeal Treatments
Authors: Ghannoum Marc , Hoffman Robert S. , Mowry James B. , Lavergne Valery 
Published: April 2014

AbstractMorbidity and mortality from toxic alcohols like ethylene glycol and methanol remain prevalent worldwide. The introduction of fomepizole, a potent blocker of alcohol dehydrogenase, has modified current practice over the last 15 years. The aim of the study was to describe the characteristics of toxic alcohol poisoning reported to US poison centers, the trends in the incidence of antidote use and hemodialysis treatment, as well as the related mortality. A retrospective study of all electronic entries from the AAPCC National Poison Data System database, from the years 2000 to 2013 was reviewed. When considering all exposures, the great majority of patients had a benign outcome. Major effects (e.g., life threatening) occurred in 2.1% and 4.9% of methanol and ethylene glycol cases, respectively. Mortality rates were similar for both toxic alcohols, approximately 0.6%. When only considering ingestions reported to healthcare facilities, a major effect was reported in 9.5% and 20.5%, and the mortality rate was 2.9% and 2.4% for methanol and ethylene glycol exposures, respectively, and remained constant over time. The use of fomepizole increased statistically over the study period while that of ethanol decreased, until it became proportionally negligible by 2012‐2013. The use of hemodialysis significantly decreased in “Early” ethylene glycol exposures during the study period. Similar to other reports, it appears that the use of fomepizole has largely supplanted ethanol as the antidote of choice in toxic alcohol exposures and may decrease the requirements for hemodialysis in patients poisoned with ethylene glycol who have no acidosis and normal kidney function.
A simple method to classify diamicts by scanning electron microscope from surface microtextures
Authors: Molén Mats O. 
Published: April 2014

AbstractInterpretation of quartz sand grain surface microtextures with scanning electron microscopy has been riddled with inconsistencies, invalid assumptions and much subjectivity. Therefore, a novel classification for analysing grain surface microtextures is presented based on the origin of complete grain surfaces. This novel method has solved most of the earlier problems of interpretation of surface microtextures, and it is easy to use and to quickly find evident genetic interpretations of diamicts. The data are plotted graphically in ‘2‐History Diagrams’ or ‘3‐History Diagrams’ for quick visual inspection and statistical evaluation. Source rocks and Quaternary glacial deposits from Scandinavia and Southern Ontario, representing different ice‐substrate dynamics, are analysed to define surface microtextures from typical glacigenic grains, bedrock and fluvially transported grains. Typical glacially crushed grains display large‐scale fractures and abrasion. Shield bedrock grains display large or small‐scale fractures and solution/precipitation microtextures. Fluvially transported grains exhibit abrasion and solution/precipitation microtextures.
The influence of four different anticoagulants on dynamic light scattering of platelets
Authors: Raczat T. , Kraemer L. , Gall C. , Weiss D. R. , Eckstein R. , Ringwald J. 
Published: April 2014

For testing of dynamic light scattering of platelets with ThromboLUX (TLX) in platelet‐rich plasma (PRP) derived from venous whole blood (vWB), anticoagulation is needed. We compared TLX score in PRPs containing citrate, ethylene‐diamine‐tetraacetic‐acid (EDTA), citrate‐phosphate‐dextrose‐adenine (CPDA) or citrate‐theophylline‐adenosine‐dipyridamole. Initial and late TLX scores were measured after 30–120 min or four to six hours, respectively. Compared with citrate, mean differences in initial TLX score were only significant for CPDA. Also, mean differences between initial and late TLX scores were only significant for CPDA. TLX failed to detect EDTA‐induced platelet alterations. The clinical relevance of TLX needs further studies.
The impact of management on weeds and aquatic plant communities in Hungarian rice crops
Authors: Pinke G , Csiky J , Mesterházy A , Tari L , Pál RW , Botta‐Dukát Z , Czúcz B 
Published: April 2014

SummaryThis study assessed the cultural and weed management factors influencing the weed communities of Hungarian rice fields. Hungary is situated at the northern limit of rice production with a history of about 300 years of rice culture. We surveyed the weed flora and 25 background variables in 100 active rice fields. Using a minimal adequate model containing 11 terms, 48.5% of the total variation in weed species data could be explained. The net effects of nine variables on species composition were significant. Crop cover was found to be the most important explanatory variable, which was followed by the herbicides penoxsulam and azimsulfuron, tillage depth, phosphorous and potassium fertilisers, years after last rotation, water depth in May, sowing type, pendimethalin and water conductivity. Filamentous algae, as the most abundant group of weeds, were positively associated with deep tillage, deep water and surface sowing. Echinochloa crus‐galli, one of the most troublesome grass weeds, was associated with low rice cover, shallow water and later years after crop rotation, while weedy rice favoured high crop cover, deep water and soil sowing. These findings can be used to design improved weed management strategies. The occurrence of red list species and charophytes in diverse micro‐mosaic patterns deserves attention from a conservation perspective, as well. The maintenance of these unique charophyte communities can be facilitated by shallow tillage without soil inversion.
Heat Transfer Enhancement Characteristics of Al2O3/Water and CuO/Water Nanofluids in a Tube in Tube Condenser Fitted With an Air Conditioning System—An Experimental Comparison
Authors: Chandraprabu V. , Sankaranarayanan G. , Iniyan S. , Suresh S. 
Published: April 2014

AbstractIn this study, heat transfer performance of nanofluids (Al2O3/water and CuO/water nanofluid) is experienced by using the condensing unit of an air conditioner. Nanoparticles at 30 nm are suspended at various volume concentrations (1%, 2%, 3%, and 4%) in the base fluid are produced for this current work. The nanofluids, considered as a cooling fluid, flow in the outer side of the tube of condenser, and general working condition of the air conditioner is applied for the investigation. Experimental results highlight the enhancement of heat transfer rate because of the existence of nanoparticles in the fluid. Two nanofluids show better heat transfer rate than does the base fluid. The Nusselt numbers for CuO/water and Al2O3/water nanofluids are enhanced up to 39.48% and 33.86%, respectively. The findings show that CuO/water nanofluids exhibit better heat transfer rate than Al2O3/water nanofluids.
Noncanonical MicroRNA (miRNA) Biogenesis Gives Rise to Retroviral Mimics of Lymphoproliferative and Immunosuppressive Host miRNAs
Authors: Kincaid Rodney P. , Chen Yating , Cox Jennifer E. , Rethwilm Axel , Sullivan Christopher S. 
Published: April 2014

ABSTRACT
MicroRNAs (miRNAs) play regulatory roles in diverse processes in both eukaryotic hosts and their viruses, yet fundamental questions remain about which viruses code for miRNAs and the functions that they serve. Simian foamy viruses (SFVs) of Old World monkeys and apes can zoonotically infect humans and, by ill-defined mechanisms, take up lifelong infections in their hosts. Here, we report that SFVs encode multiple miRNAs via a noncanonical mode of biogenesis. The primary SFV miRNA transcripts (pri-miRNAs) are transcribed by RNA polymerase III (RNAP III) and take multiple forms, including some that are cleaved by Drosha. However, these miRNAs are generated in a context-dependent fashion, as longer RNAP II transcripts spanning this region are resistant to Drosha cleavage. This suggests that the virus may avoid any fitness penalty that could be associated with viral genome/transcript cleavage. Two SFV miRNAs share sequence similarity and functionality with notable host miRNAs, the lymphoproliferative miRNA miR-155 and the innate immunity suppressor miR-132. These results have important implications regarding foamy virus biology, viral miRNAs, and the development of retroviral-based vectors.

IMPORTANCE
Fundamental questions remain about which viruses encode miRNAs and their associated functions. Currently, few natural viruses with RNA genomes have been reported to encode miRNAs. Simian foamy viruses are retroviruses that are prevalent in nonhuman host populations, and some can zoonotically infect humans who hunt primates or work as animal caretakers. We identify a cluster of miRNAs encoded by SFV. Characterization of these miRNAs reveals evolutionarily conserved, unconventional mechanisms to generate small RNAs. Several SFV miRNAs share sequence similarity and functionality with host miRNAs, including the oncogenic miRNA miR-155 and innate immunity suppressor miR-132. Strikingly, unrelated herpesviruses also tap into one or both of these same regulatory pathways, implying relevance to a broad range of viruses. These findings provide new insights with respect to foamy virus biology and vectorology.
Antibody-Mediated Inhibition of Ricin Toxin Retrograde Transport
Authors: Yermakova Anastasiya , Klokk Tove Irene , Cole Richard , Sandvig Kirsten , Mantis Nicholas J. 
Published: April 2014

ABSTRACTRicin is a member of the ubiquitous family of plant and bacterial AB toxins that gain entry into the cytosol of host cells through receptor-mediated endocytosis and retrograde traffic through thetrans-Golgi network (TGN) and endoplasmic reticulum (ER). While a few ricin toxin-specific neutralizing monoclonal antibodies (MAbs) have been identified, the mechanisms by which these antibodies prevent toxin-induced cell death are largely unknown. Using immunofluorescence confocal microscopy and a TGN-specific sulfation assay, we demonstrate that 24B11, a MAb against ricin’s binding subunit (RTB), associates with ricin in solution or when prebound to cell surfaces and then markedly enhances toxin uptake into host cells. Following endocytosis, however, toxin-antibody complexes failed to reach the TGN; instead, they were shunted to Rab7-positive late endosomes and LAMP-1-positive lysosomes. Monovalent 24B11 Fab fragments also interfered with toxin retrograde transport, indicating that neither cross-linking of membrane glycoproteins/glycolipids nor the recently identified intracellular Fc receptor is required to derail ricin en route to the TGN. Identification of the mechanism(s) by which antibodies like 24B11 neutralize ricin will advance our fundamental understanding of protein trafficking in mammalian cells and may lead to the discovery of new classes of toxin inhibitors and therapeutics for biodefense and emerging infectious diseases.IMPORTANCERicin is the prototypic member of the AB family of medically important plant and bacterial toxins that includes cholera and Shiga toxins. Ricin is also a category B biothreat agent. Despite ongoing efforts to develop vaccines and antibody-based therapeutics against ricin, very little is known about the mechanisms by which antibodies neutralize this toxin. In general, it is thought that antibodies simply prevent toxins from attaching to cell surface receptors or promote their clearance through Fc receptor (FcR)-mediated uptake. In this report, however, we describe a neutralizing monoclonal antibody (MAb) against ricin’s binding subunit (RTB) that not only associates with ricin after the toxin has bound to the cell’s surface but actually enhances toxin uptake into host cells. Following endocytosis, the antibody-toxin complexes are then routed for degradation. The results of this study are important because they reveal a previously unappreciated role for B-subunit-specific antibodies in intracellular neutralization of ricin toxin.
Novel Determinants of Antibiotic Resistance: Identification of Mutated Loci in Highly Methicillin-Resistant Subpopulations of Methicillin-Resistant Staphylococcus aureus
Authors: Dordel Janina , Kim Choonkeun , Chung Marilyn , Pardos de la Gándara María , Holden Matthew T. J. , Parkhill Julian , de Lencastre Hermínia , Bentley Stephen D. , Tomasz Alexander 
Published: April 2014

ABSTRACT

We identified mutated genes in highly resistant subpopulations of methicillin-resistant
Staphylococcus aureus
(MRSA) that are most likely responsible for the historic failure of the β-lactam family of antibiotics as therapeutic agents against these important pathogens. Such subpopulations are produced during growth of most clinical MRSA strains, including the four historically early MRSA isolates studied here. Chromosomal DNA was prepared from the highly resistant cells along with DNA from the majority of cells (poorly resistant cells) followed by full genome sequencing. In the highly resistant cells, mutations were identified in 3 intergenic sequences and 27 genes representing a wide range of functional categories. A common feature of these mutations appears to be their capacity to induce high-level β-lactam resistance and increased amounts of the resistance protein PBP2A in the bacteria. The observations fit a recently described model in which the ultimate controlling factor of the phenotypic expression of β-lactam resistance in MRSA is a RelA-mediated stringent response.

IMPORTANCE
It has been well established that the level of antibiotic resistance (i.e., minimum concentration of a β-lactam antibiotic needed to inhibit growth) of a methicillin-resistant
Staphylococcus aureus
(MRSA) strain depends on the transcription and translation of the resistance protein PBP2A. Here we describe mutated loci in an additional novel set of genetic determinants that appear to be essential for the unusually high resistance levels typical of subpopulations of staphylococci that are produced with unique low frequency in most MRSA clinical isolates. We propose that mutations in these determinants can trigger induction of the stringent stress response which was recently shown to cause increased transcription/translation of the resistance protein PBP2A in parallel with the increased level of resistance.
Non-steroidal anti-inflammatory drugs and the risk of atrial fibrillation: a population-based follow-up study
Authors: Krijthe Bouwe P , Heeringa Jan , Hofman Albert , Franco Oscar H , Stricker Bruno H 
Published: April 2014

ObjectiveTo investigate the association of non-steroidal anti-inflammatory drugs (NSAIDs) and the risk of atrial fibrillation in a prospective community-based follow-up study of elderly individuals with uniform case assessment and data on potential confounders.DesignData came from the population-based follow-up study, the Rotterdam Study.ParticipantsThe study comprised 8423 participants without atrial fibrillation at baseline.Main outcome measuresAtrial fibrillation was ascertained from ECG assessments as well as medical records. Use of NSAIDs was obtained from automated prescription records by linkage with participating pharmacies. We used Cox proportional hazards models to study the association between NSAID drug use and atrial fibrillation. Use of NSAIDs was included in the model as a time-varying variable.ResultsAt baseline, the mean age of the study population was 68.5 years (SD: 8.7) and 58% were women. During a mean follow-up of 12.9 years, 857 participants developed atrial fibrillation. Current use of NSAIDs was associated with increased risk compared with never-use (HR 1.76, 95% CI 1.07 to 2.88). Also, recent use (within 30 days after discontinuation of NSAIDs) was associated with an increased risk of atrial fibrillation compared with never-use (HR 1.84, 95% CI 1.34 to 2.51) adjusted for age, sex and several potential confounders.ConclusionsIn this study, use of NSAIDs was associated with an increased risk of atrial fibrillation. Further studies are needed to investigate the underlying mechanisms behind this association.
Psychiatric disorders among men voluntarily in treatment for violent behaviour: a cross-sectional study
Authors: Askeland Ingunn Rangul , Heir Trond 
Published: April 2014

ObjectivesAlthough violent behaviour and psychopathology often co-occur, there has been little research on psychiatric disorders among men in treatment for intimate partner violence (IPV). This study aimed to examine the prevalence of a broad spectrum of psychiatric disorders among men voluntarily attending treatment for IPV.Setting5 clinics for IPV treatment, located in the east, south and west of Norway, participated in the study. In a cross-sectional design, men attending therapy for violence against a partner went through a face-to-face structured diagnostic interview, the Mini International Neuropsychiatric Interview.Participants222 men contacted the clinic during the inclusion period; 12 men did not attend and 13 men were referred to outpatient clinics. Of the 197 men who were offered therapy, 13 did not provide consent to participate in the study, 2 were excluded and 3 men missed the interview.ResultsA total of 179 men participated in the study. The majority were ethnic Norwegians (88%). A total of 70.9% of the men fulfilled the diagnostic criteria for at least one ongoing psychiatric disorder. Three categories of disorders stood out with approximately equal prevalences: depressive disorders (40.6%), anxiety disorders (38.5%) including post-traumatic stress disorder (18.4%) and alcohol/substance abuse (40.2%). Antisocial personality disorder was present in approximately 2/10 participants. Comorbidity was high, with nearly half of the men (48.0%) assigned two or more diagnoses.ConclusionsMen voluntarily admitted to treatment for IPV harbour a wide spectrum of psychiatric disorders. Our findings suggest a need for screening procedures for psychiatric disorders as well as adoption of treatment interventions according to different types of psychopathologies and therapeutic needs. Limitations include caution in terms of generalisation to other populations not voluntarily admitted to treatment for IPV, and risk of ignoring symptoms not covered by a clinical structured interview.
Exposure to combined oral contraceptives and risk of venous thromboembolism: a protocol for nested case–control studies using the QResearch and the CPRD databases
Authors: Vinogradova Yana , Coupland Carol , Hippisley-Cox Julia 
Published: April 2014

IntroductionMany studies have found an increased risk of venous thromboembolism (VTE) associated with the use of combined hormonal contraceptives, but various methodologies have been used in the study design relating to definition of VTE event and the selection of appropriate cases for analysis. This study will focus on common oral hormonal contraceptives, including compositions with cyproterone because of their contraceptive effect and will perform a number of sensitivity analyses to compare findings with previous studies.Methods and analysis2 nested case–control studies will be based on the general population using records from UK general practices within the QResearch and Clinical Practice Research Datalink databases. Cases will be female patients aged 15–49 with primary VTE diagnosed between 2001 and 2013. Each case will be matched by age, year of birth and practice to five female controls, who are alive and registered with the practice at the time of diagnosis of the case (index date). Exposure to different hormonal contraceptives will be defined as at least one prescription for that contraceptive in the year before the index date. The effects of duration and the length of any gap since last use will also be investigated. Conditional logistic regression will be applied to calculate ORs adjusted for smoking, ethnicity, comorbidities and use of other medications. Possible indications for prescribing hormonal contraceptives, such as menstrual disorders, acne or hirsutism will be included in the analyses as confounding factors. A number of sensitivity analyses will be carried out.Ethics and disseminationThe initial protocol has been reviewed and approved by ISAC (Independent Scientific Advisory Committee) for Medicine and Healthcare Products Regulatory Agency Database Research. The project has also been reviewed by QResearch and meets the requirements of the Trent Research Ethics Committee. The results will be published in a peer-reviewed journal.
Diagnostic quality and accuracy of low dose 3D-DSA protocols in the evaluation of intracranial aneurysms
Authors: Pearl Monica S , Torok Collin , Katz Zinovy , Messina Steven A , Blasco Jordi , Tamargo Rafael J , Huang Judy , Leigh Richard , Zeiler Steven , Radvany Martin , Ehtiati Tina , Gailloud Philippe 
Published: April 2014

Background3D-DSA is the ‘gold standard’ imaging technique for the diagnosis and characterization of intracranial aneurysms.ObjectiveTo compare the image quality and accuracy of low dose 3D-DSA protocols in patients with unruptured intracranial aneurysms.Materials and methodsThe standard manufacturer 5 s 0.36 μGy/f protocol and one of three low dose 3D-DSA protocols (5 s 0.10 μGy/f, 5 s 0.17 μGy/f, 5 s 0.24 μGy/f) were performed in 12 patients with unruptured intracranial aneurysms. Three interventional neuroradiologists, two neurosurgeons, and two neurologists rated the image quality of all 3D reconstructions as good, acceptable, or poor. Three interventional neuroradiologists measured two dimensions of each aneurysm for all protocols. The radiation dose metric Ka,r (reference point air kerma, in mGy) was recorded for each 3D-DSA protocol.ResultsThe standard 5 s 0.36 μGy/f protocol earned the highest average subjective rating of 2.76, followed by the 5 s 0.24 μGy/f (2.72), and 5 s 0.17 μGy/f (2.59) protocols. The ranges of differences in aneurysm measurements between the 5 s 0.24 μGy/f protocol and the standard were <0.5 mm. The median Ka,r metrics for each protocol were as follows: 5 s 0.36 μGy/f (89.0 mGy), 5 s 0.24 μGy/f (57.7 mGy), 5 s 0.17 μGy/f (45.9 mGy), and 5 s 0.10 μGy/f (27.6 mGy).ConclusionsLow dose 3D-DSA protocols with preserved image quality are achievable, and can help reduce exposure of patients and operators to unnecessary radiation. The 5 s 0.24 μGy/f protocol generates one-third smaller radiation dose than the standard 5 s 0.36 μGy/f protocol without compromising diagnostic image quality or accuracy.
N-butyl cyanoacrylate embolization using a detachable tip microcatheter: initial experience
Authors: Paramasivam Srinivasan , Altschul David , Ortega-Gutiarrez Santiago , Fifi Johanna , Berenstein Alejandro 
Published: April 2014

IntroductionEndovascular embolization of intracranial vascular malformations with N-butyl cyanoacrylate (nBCA) using a detachable tip microcatheter allows prolonged injection and decreases the risk of catheter retention.MethodsBetween March and December 2013, the Apollo 1.5 cm detachable tip microcatheter was used in five patients after being approved by both the Food and Drug Administration and the institutional review board as a compassionate use device. Nine pedicles were embolized and the follow-up ranged from 1 to 3 months.ResultsFive of the nine catheter tips detached. The length of reflux was not directly associated with the detachment of the distal tip. There were no cases of premature microcatheter detachment during navigation, manipulation with multiple microguidewire reintroduction and guidance. There was no leak of embolic agent at the detachment zone. Follow-up showed the detached tip to be stable without migration.ConclusionsA detachable tip microcatheter offers an advance in the safety and effectiveness of nBCA embolization. Catheter retrieval becomes more controlled and less traumatic. Our initial experience is encouraging, and more experience is needed to categorically ascertain its safety and efficacy.