bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reaction-based fluorescent sensor for investigating mobile Zn
2+
in mitochondria of healthy versus cancerous prostate cells
Authors: Chyan Wen , Zhang Daniel Y. , Lippard Stephen J. , Radford Robert J. 
Published: December 2013

Significance
Mobile zinc plays important roles in mammalian physiology. Understanding the action of mobile zinc requires tools to follow it within and between live cells. Zinc-selective fluorescent probes offer a facile means for detecting such mobile zinc, but small molecules constructed to perform this task typically have an unpredictable cellular distribution. Subtle changes in chemical structure can radically alter subcellular localization. To overcome this challenge, we installed a chemical unit to direct the sensor specifically to mitochondria and discovered that tumorigenic cells lose their ability to accumulate mobile zinc within these organelles. To carry out this work, we devised a reaction-based sensor that undergoes zinc-mediated chemistry, converting a nonfluorescent molecule into one that emits brightly and avoiding undesired sequestration in endo/lysosome.
A combinatorial approach to graphlet counting
Authors: Hočevar Tomaž , Demšar Janez 
Published: December 2013

Abstract
Motivation: Small-induced subgraphs called graphlets are emerging as a possible tool for exploration of global and local structure of networks and for analysis of roles of individual nodes. One of the obstacles to their wider use is the computational complexity of algorithms for their discovery and counting.
Results: We propose a new combinatorial method for counting graphlets and orbit signatures of network nodes. The algorithm builds a system of equations that connect counts of orbits from graphlets with up to five nodes, which allows to compute all orbit counts by enumerating just a single one. This reduces its practical time complexity in sparse graphs by an order of magnitude as compared with the existing pure enumeration-based algorithms.
Availability and implementation: Source code is available freely at http://www.biolab.si/supp/orca/orca.html.
Contact: tomaz.hocevar@fri.uni-lj.si
Supplementary information: Supplementary data are available at Bioinformatics online.
Genomics in Coccidioides: Insights into evolution, ecology, and pathogenesis
Authors: Whiston Emily , Taylor John W 
Published: December 2013

AbstractCoccidioides immitis and C. posadasii, the causative agents of the mammalian disease coccidioidomycosis, are dimorphic fungal pathogens distributed throughout desert-like environments in North and South America. Coccidioides spp. are members of the Onygenales, a diverse group of pathogenic and nonpathogenic fungi. Recently, full genomes have been published for Coccidioides and a number of other Onygenales species. Phylogenomic comparisons and additional studies in Coccidioides population genomics and gene expression have shed light on the ecology and pathogenesis of Coccidioides and the other medically important species in this clade. Observed patterns of gene family expansion/contraction and evidence of gene flow have provided insight to the evolution of Coccidioides and greatly broadened our understanding of the diversity and sources of genetic variation found in fungi. In the future, expansion of the number of sequenced isolates from all populations will allow deeper insight into the evolutionary processes that have shaped this unique human pathogen. In addition, deep sequencing of isolates from a single Coccidioides population and pairing of those data with phenotype information on growth and pathogenicity for genome-wide association analysis will allow researchers to find genes responsible for any phenotype, virulence included, that shows variation in the population.
Small RNAs reveal two target sites of the RNA-maturation factor Mbb1 in the chloroplast of Chlamydomonas
Authors: Loizeau Karen , Qu Yujiao , Depp Sébastien , Fiechter Vincent , Ruwe Hannes , Lefebvre-Legendre Linnka , Schmitz-Linneweber Christian , Goldschmidt-Clermont Michel 
Published: December 2013

Abstract
Many chloroplast transcripts are protected against exonucleolytic degradation by RNA-binding proteins. Such interactions can lead to the accumulation of short RNAs (sRNAs) that represent footprints of the protein partner. By mining existing data sets of Chlamydomonas reinhardtii small RNAs, we identify chloroplast sRNAs. Two of these correspond to the 5′-ends of the mature psbB and psbH messenger RNAs (mRNAs), which are both stabilized by the nucleus-encoded protein Mbb1, a member of the tetratricopeptide repeat family. Accordingly, we find that the two sRNAs are absent from the mbb1 mutant. Using chloroplast transformation and site-directed mutagenesis to survey the psbB 5′ UTR, we identify a cis-acting element that is essential for mRNA accumulation. This sequence is also found in the 5′ UTR of psbH, where it plays a role in RNA processing. The two sRNAs are centered on these cis-acting elements. Furthermore, RNA binding assays in vitro show that Mbb1 associates with the two elements specifically. Taken together, our data identify a conserved cis-acting element at the extremity of the psbH and psbB 5′ UTRs that plays a role in the processing and stability of the respective mRNAs through interactions with the tetratricopeptide repeat protein Mbb1 and leads to the accumulation of protected sRNAs.
The Agricultural Productivity Gap *
Authors: Gollin Douglas , Lagakos David , Waugh Michael E. 
Published: December 2013

AbstractAccording to national accounts data, value added per worker is much higher in the nonagricultural sector than in agriculture in the typical country, particularly in developing countries. Taken at face value, this “agricultural productivity gap” suggests that labor is greatly misallocated across sectors. In this article, we draw on new micro evidence to ask to what extent the gap is still present when better measures of sector labor inputs and value added are taken into consideration. We find that even after considering sector differences in hours worked and human capital per worker, as well as alternative measures of sector output constructed from household survey data, a puzzlingly large gap remains.
China's educational cooperation with Africa: toward new strategic partnerships
Authors: Niu Changsong 
Published: December 2013

Purpose– The purpose of this paper is to examine China-Africa educational cooperation under the framework of Forum on China-Africa Cooperation (FOCAC) while illustrating the modalities of Sino-Africa educational cooperation.Design/methodology/approach– Based on empirical and documentary analysis, the paper describes and explores the unique features of China-Africa educational cooperation.Findings– In the past decades, China's expanding engagement with Africa in the field of international development assistance has attracted great attention and given rise to mixed reactions and arguments at the international level. China's cooperation with Africa has a long and notable history, dating back to the 1950s. China's cooperation with other developing countries is known as South-South development cooperation, based on principles of equality, mutual benefit, solidarity and no conditionality. With a thorough and critical analysis of the decision-making mechanism and the practices of China's educational cooperation with Africa, this paper puts forward some important issues regarding the perspective of sustainability and effectiveness in Chinese cooperative arrangements.Research limitations/implications– The paper tries to explain the dynamic and the practices of China's education engagement with Africa and puts forward key challenges regarding its effectiveness toward new strategic partnerships. However, the answers to these questions require some innovative measures in the future practices of China-Africa educational cooperation.Originality/value– The China-Africa education partnership is not a stand-alone sector, which can be better understood in the context of the historical development of China-Africa cooperation and under the framework of the FOCAC.
Overlap between autism and schizophrenia: history and current status
Authors: Fitzgerald Michael 
Published: December 2013

Purpose– Considerable confusion surrounds the overlapping of autism and schizophrenia. This has significant implications for clinicians given that correct diagnosis is critical for treatment.Design/methodology/approach– This paper sets out to clarify the position by reviewing the history and current status of the relationship between autism and schizophrenia. A general review was conducted using a chronological approach that focused on phenomenology, aetiology, genetic mechanisms and treatment.Findings– Persons with autism are far more rigid, have difficulties set shifting and get far more upset and aggressive when their routines have changed. They have far more severe theory of mind and empathy deficits than those with schizophrenia.Research limitations/implications– Future diagnostic refinement by means of molecular genetic studies will alter the diagnostic categories. Further studies of the conditions of autism and schizophrenia are therefore necessary.Practical implications– Both conditions need treatment both clinically and practically.Originality/value– This paper elucidates the relationship between autism and schizophrenia from a historical and current perspective. It emerges that this confusion is likely to be resolved by molecular genetic studies that will alter the diagnostic categories.
Attenuating double jeopardy of negative country of origin effects and latecomer brand
Authors: Hamin Hamin , Baumann Chris , L. Tung Rosalie 
Published: December 2013

Purpose
– The purpose of this paper is to examine the role of ethnocentrism in attenuating the negative country of origin effect and latecomer brands. The literature has established the importance of the “country of origin” effect, and this study compares consumers in the Asian emerging markets to developed consumers' response to cars from China, India and Russia.

Design/methodology/approach
– Data on consumers' willingness to purchase cars from emerging markets such as China, India and Russia were collected from 3,201 respondents in those three emerging markets and in the three most important Western car markets, the USA, the UK and Germany. The study employed a choice-based conjoint analysis.

Findings
– The results of this study confirmed the hypothesised ethnocentrism in the emerging markets with a strong preference for their own latecomer brands (Great Wall, Tata and AvtoVAZ, respectively). Developed markets in contrast are more sceptical of the Chinese, Indian and Russian car brands, but there is nonetheless substantial potential, especially with consumers who have previously bought latecomer brands from Asia. Utility values per brand, price, brand-partnership, product features, warranties and also place of manufacturing/assembly have been calculated in the study.

Originality/value
– This paper should prove valuable to academic researchers in establishing strong consumer preferences in emerging markets for their own products, and in establishing the potential of latecomer brands in developed markets.
Integrated systems oriented student-centric learning environment
Authors: S. Desai Mayur , A. Johnson Ronald 
Published: December 2013

Purpose
– The purpose of this paper is to propose a framework that serves as a guide to develop a curriculum and instructional strategy that is systems oriented and student-centric.

Design/methodology/approach
– The framework is based on the theories in the field of education by prominent researchers. The framework is divided into four sub-systems, input, process, output, and feedback. While the input, process, and output sections discuss basic elements critical in students’ learning, the feedback section suggests how the learning environment can be controlled and corrected based on student learning outcomes.

Findings
– The paper presents a framework for the student to graduate transformation process in institutions of higher education based on fundamental premise that this process is an ecosystem consisting of several interlinked and interacting sub-systems. A key driver of the transformation process should be a feasible set of expected competencies that the global market place for talent expects of college graduates. These market-valued competencies can be used to define the success of the curriculum, course design, and instructional techniques and determine the sufficiency of the resource allocation by the institution of higher education to specific academic and supporting programs.

Originality/value
– The framework that is based on integration of well-grounded theories in the field of education. Thus it adds value to the development of curriculum that is needed by the education institutions to excel and compete in the global and dynamic academic environment.
Sustainability in the global hotel industry
Authors: Jones Peter , Hillier David , Comfort Daphne 
Published: December 2013

Purpose
– The purpose of this paper is to provide an exploratory review of how the global hotel industry publicly communicates its approach to sustainability.

Design/methodology/approach
– The paper begins with an outline of the growing importance of sustainability within the hospitality industry and a short discussion of the characteristics of sustainability. The paper draws its empirical material from the most recent information on sustainability posted on the world's leading hotel chains' corporate websites.

Findings
– The findings reveal that while there is considerable variation in the information the leading hotel chains provided publicly on their sustainability commitments and achievements, they embrace a wide range of environmental, social and economic issues. More critically, the authors argue that these commitments are driven more by the search for efficiency gains, that they are couched within existing business models centred on continuing growth, and that as such the global hotel industry is currently pursuing a “weak” rather than a “strong” model of sustainability.

Practical implications
– The paper suggests that although the leading global hotel companies are well placed to take a leading role in promoting sustainability, they may need to extend their sustainability reporting and to introduce external assurance procedures.

Originality/value
– The paper provides an accessible review of the sustainability agendas currently being pursued within the global hotel industry and as such it will interest academics, students and practitioners interested in both the hospitality industry and corporate sustainability strategies.
Managing organizational challenges in global projects
Authors: Aarseth Wenche , Rolstadås Asbjørn , Andersen Bjorn 
Published: December 2013

Purpose– The purpose of this paper is to complement the research that has been done in global projects so far and has two objectives: to study organizational challenges in global projects, compared with those of traditional projects; and to define and analyze the main organizational challenges the project team members and project managers meet when assigned to global projects.Design/methodology/approach– The research is based on a survey sent to 550 project managers and people working in a global environment, data from 246 respondents, and 30 interviews with senior project team members.Findings– The results show that the main organizational challenges are managing the external stakeholders in the global project; the local government in the country, local content demand, local authorities, local industry, and lack of support from the base organization and management. One of the conclusions is that companies need a relationship management approach to managing these challenges in global projects.Originality/value– Organizational challenges are an underestimated area in projects and when it comes to an in-depth understanding of organizational challenges in global projects only a very few studies have been published compared with other project management issues. This article contributes to existing research by presenting the organizational challenges in global projects and how they differ from traditional projects.
Greening the campus of a Brazilian university: cultural challenges
Authors: da Silveira Marques Pereira Giovana , Jabbour Charbel , V.W. Borges de Oliveira Sonia , Alves Teixeira Adriano 
Published: December 2013

Purpose
– This study examined the relationship between environmental management practices developed at a campus of a Brazilian university (University of Sao Paulo) and the greening of its organizational culture. The paper aims to discuss these issues.

Design/methodology/approach
– This article presents a theoretical background based on the concepts of environmental management, organizational culture and environmental management in higher education institutions. The main framework of this research is the model proposed by Harris and Crane.

Findings
– The studied university has an environmental management program that is sometimes constrained in the following ways: the university bureaucracy and hierarchy; the main performance indicators for lecturers and professors are based on scientific production and publication, giving them little time for complementary activities; and some units develop their own environmental management practices, but they are not disseminated as best practices for use by other units. Some academic units showcase the proactive actions of professors who incorporate environmental management into their daily activities. The general perception is that the phrase “environmental management” is almost synonymous with “solid waste management”.

Originality/value
– This research details the first Brazilian application of the Harris and Crane model. It contributes an original analysis of environmental management and “green” organizational culture of a Brazilian university, an organizational type that has seldom been studied to date.
A high gain and high linearity class-AB power amplifier for WCDMA applications
Authors: Ramiah Harikrishnan , Eswaran U. , Kanesan J. 
Published: December 2013

Purpose
– The purpose of this paper is to design and realize a high gain power amplifier (PA) with low output back-off power using the InGaP/GaAs HBT process for WCDMA applications from 1.85 to 1.91 GHz.

Design/methodology/approach
– A three stages cascaded PA is designed which observes a high power gain. A 100 mA of quiescent current helps the PA to operate efficiently. The final stage device dimension has been selected diligently in order to deliver a high output power. The inter-stage match between the driver and main stage has been designed to provide maximum power transfer. The output matching network is constructed to deliver a high linear output power which meets the WCDMA adjacent channel leakage ratio (ACLR) requirement of −33 dBc close to the 1 dB gain compression point.

Findings
– With the cascaded topology, a maximum 31.3 dB of gain is achieved at 1.9 GHz. S11 of less than −18 dB is achieved across the operating frequency band. The maximum output power is indicated to be 32.7 dBm. An ACLR of −33 dBc is achieved at maximum linear output power of 31 dBm.

Practical implications
– The designed PA is an excellent candidate to be employed in the WCDMA transmitter chain without the aid of additional driver amplifier and linearization circuits.

Originality/value
– In this work, a fully integrated GaAs HBT PA has been implemented which is capable to operate linearly close to its 1 dB gain compression point.
Leadership networks in destinations
Authors: Zehrer Anita , Raich Frieda , Siller Hubert , Tschiderer Franz 
Published: December 2013

Purpose– Co-operation activities play a central role for the development of a tourism destination, and require a co-operative approach among numerous different actors to manage and market a tourism destination. This paper aims to describe the characteristics of leadership networks in tourism destinations and their impact on destination development.Design/methodology/approach– The paper discusses network relationships of destination leaders, as in their collectivity, leaders shape and influence the destination's core services, strategic orientation and innovation capability. The research is based on the examination of relevant literature and a quantitative survey conducted in five selected tourism destinations in the Tirol, Austria.Findings– The article discusses leadership networks in community-structured destinations, providing insights into its organizational structure and dynamic behaviour. Moreover, the paper illustrates the network characteristics and its influence on the development of the tourism destination. Thus, implications for destination management can be derived.Originality/value– Discussions on leadership have usually been limited to firms and have not yet fully embraced the network and destination level. The originality of the paper is to provide insights in destination leadership and networking activities of leaders within destinations by means of a quantitative approach and thus adds to the growing body of literature on the functionalities of destination leadership networks, their structures and mechanisms.
Novel molecular determinants of viral susceptibility and resistance in the lipidome of Emiliania huxleyi
Authors: Fulton James M. , Fredricks Helen F. , Bidle Kay D. , Vardi Assaf , Kendrick B. Jacob , DiTullio Giacomo R. , Van Mooy Benjamin A. S. 
Published: December 2013

SummaryViruses play a key role in controlling the population dynamics of algae, including Emiliania huxleyi, a globally distributed haptophyte with calcite coccoliths that comprise ca. 50% of the sinking carbonate flux from the surface ocean. Emiliania huxleyi viruses (EhVs) routinely infect and terminate E. huxleyi blooms. EhVs are surrounded by a lipid envelope, which we found to be comprised largely of glycosphingolipids (GSLs) with lesser amounts of polar glycerolipids. Infection appears to involve membrane fusion between the virus and host, and we hypothesized that specific polar lipids may facilitate virus attachment. We identified three novel intact polar lipids in E. huxleyi strain CCMP 374 and EhV86, including a GSL with a monosaccharide sialic acid headgroup (sGSL); for all 11 E. huxleyi strains we tested, there was a direct relationship between sGSL content and sensitivity to infection by EhV1, EhV86 and EhV163. In mesocosms, the E. huxleyi population with greatest initial sGSL content had the highest rate of virus‐induced mortality. We propose potential physiological roles for sGSL that would be beneficial for growth but leave cells susceptible to infection, thus furthering the discussion of Red Queen‐based co‐evolution and the cost(s) of sensitivity and resistance in the dynamic E. huxleyi‐EhV system.
Psychopathy and the Cinema: Fact or Fiction?
Authors: Leistedt Samuel J. , Linkowski Paul 
Published: December 2013

AbstractThe authors investigated the relationship between cinema and psychopathy to describe and analyze the portrayal of fictional psychopathic characters in popular films and over cinematic history. From 400 films (1915–2010), 126 fictional psychopathic characters (21 female and 105 male) were selected based on the realism and clinical accuracy of their profiles. Movies were then analyzed by senior forensic psychiatrists and cinema critics. Secondary (71%) and manipulative (48%) subtypes were the most common in the female group, while secondary (51%) and prototypical (34%) were the most common in the male group. Corresponding to the increased understanding of clinical psychopathy by professional mental health providers over time, the clinical description of and epidemiological data on fictional psychopaths in popular films have become more realistic. Realistic fictional psychopaths remain in the minority but are very important for didactic purposes in Academic facilities, as “teaching Movies.”