bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transapical aortic valve replacement complicated by periaortic hematoma
Authors: Naji Darwish I. , Chhatriwalla Adnan , Cohen David J. , Main Michael L. 
Published: July 2015

Abstract
A 77-year-old woman with severe symptomatic aortic stenosis (aortic valve areaZ0.6 cm2 and mean gradientZ 53 mmHg) was deemed inoperable for surgical aortic valve replacement due to a porcelain aorta, and was scheduled for transcatheter aortic valve replacement via an apical approach. A 23 mm valve was selected based on a computerized axial tomographic angiography derived area of 360 mm2; valve oversizing was 15.4%. Baseline transesophageal echocardiography revealed severe protruding atheroma within the lumen of the ascending aorta. Immediately following deployment of the Sapien pericardial tissue valve (Edwards Life Sciences, Irvine, CA, USA), a large posterior periaortic hematoma was noted (1.3 cm in maximum width, and w4 cm in length) (see Fig. 1 and Videos 1, 2, 3, 4). Protamine was administered, and the hematoma was observed by transesophegeal echocardiography for w30 min with no change in size; function of the bioprosthetic valve was normal without regurgitation. In the immediate post-operative period, anti-platelet agents were withheld, and blood pressure was aggressively lowered. The remainder of her hospital course was uncomplicated, and a tranthoracic echocardiogram w1 month later demonstrated resolution of the hematoma. Periaortic hematoma and aortic root rupture are recognized potential major complications of transcatheter aortic valve replacement (1, 2). Severe left ventricular outflow tract calcification and annular over sizing are both risk factors for these complications. Careful transesophageal echocardiographic examination post valve deployment may lead to early recognition and treatment of this potentially fatal complication (3). In this case, presence of severe protruding atherosclerotic plaque pre-procedure likely predisposed to this complication.
Endogenous Small RNA Mediates Meiotic Silencing of a Novel DNA Transposon
Authors: Wang Yizhou , Smith Kristina M , Taylor John W , Freitag Michael , Stajich Jason E 
Published: July 2015

AbstractGenome defense likely evolved to curtail the spread of transposable elements and invading viruses. A combination of effective defense mechanisms has been shown to limit colonization of the Neurospora crassa genome by transposable elements. A novel DNA transposon named Sly1-1 was discovered in the genome of the most widely used laboratory “wild-type” strain FGSC 2489 (OR74A). Meiotic silencing by unpaired DNA, also simply called meiotic silencing, prevents the expression of regions of the genome that are unpaired during karyogamy. This mechanism is posttranscriptional and is proposed to involve the production of small RNA, so-called masiRNAs, by proteins homologous to those involved in RNA interference−silencing pathways in animals, fungi, and plants. Here, we demonstrate production of small RNAs when Sly1-1 was unpaired in a cross between two wild-type strains. These small RNAs are dependent on SAD-1, an RNA-dependent RNA polymerase necessary for meiotic silencing. We present the first case of endogenously produced masiRNA from a novel N. crassa DNA transposable element.
On the Mechanism of Gene Silencing in Saccharomyces cerevisiae
Authors: Steakley David Lee , Rine Jasper 
Published: July 2015

Abstract
Multiple mechanisms have been proposed for gene silencing in Saccharomyces cerevisiae, ranging from steric occlusion of DNA binding proteins from their recognition sequences in silenced chromatin to a specific block in the formation of the preinitiation complex to a block in transcriptional elongation. This study provided strong support for the steric occlusion mechanism by the discovery that RNA polymerase of bacteriophage T7 could be substantially blocked from transcribing from its cognate promoter when embedded in silenced chromatin. Moreover, unlike previous suggestions, we found no evidence for stalled RNA polymerase II within silenced chromatin. The effectiveness of the Sir protein–based silencing mechanism to block transcription activated by Gal4 at promoters in the domain of silenced chromatin was marginal, yet it improved when tested against mutant forms of the Gal4 protein, highlighting a role for specific activators in their sensitivity to gene silencing.
Gene Model Annotations forDrosophila melanogaster: The Rule-Benders
Authors: Crosby Madeline A , Gramates L Sian , dos Santos Gilberto , Matthews Beverley B , St. Pierre Susan E , Zhou Pinglei , Schroeder Andrew J , Falls Kathleen , Emmert David B , Russo Susan M , Gelbart William M ,   the FlyBase Consortium
Published: July 2015

AbstractIn the context of the FlyBase annotated gene models in Drosophila melanogaster, we describe the many exceptional cases we have curated from the literature or identified in the course of FlyBase analysis. These range from atypical but common examples such as dicistronic and polycistronic transcripts, noncanonical splices, trans-spliced transcripts, noncanonical translation starts, and stop-codon readthroughs, to single exceptional cases such as ribosomal frameshifting and HAC1-type intron processing. In FlyBase, exceptional genes and transcripts are flagged with Sequence Ontology terms and/or standardized comments. Because some of the rule-benders create problems for handlers of high-throughput data, we discuss plans for flagging these cases in bulk data downloads.
A Forward Genetic Screen for Suppressors of Somatic P Granules inCaenorhabditis elegans
Authors: Kelly Ashley L , Senter-Zapata Michael J , Campbell Anne C , Lust Hannah E , Theriault Monique E , Andralojc Karolina M , Updike Dustin L 
Published: July 2015

AbstractIn Caenorhabditis elegans, germline expression programs are actively repressed in somatic tissue by components of the synMuv (synthetic multi-vulva) B chromatin remodeling complex, which include homologs of tumor suppressors Retinoblastoma (Rb/LIN-35) and Malignant Brain Tumor (MBT/LIN-61). However, the full scope of pathways that suppress germline expression in the soma is unknown. To address this, we performed a mutagenesis and screened for somatic expression of GFP-tagged PGL-1, a core P-granule nucleating protein. Eight alleles were isolated from 4000 haploid genomes. Five of these alleles exhibit a synMuv phenotype, whereas the remaining three were identified as hypomorphic alleles of known synMuv B genes, lin-13 and dpl-1. These findings suggest that most suppressors of germline programs in the soma of C. elegans are either required for viability or function through synMuv B chromatin regulation.
Comparative Transcriptome Analysis of the Cosmopolitan Marine Fungus Corollospora maritima Under Two Physiological Conditions
Authors: Velez Patricia , Alejandri-Ramírez Naholi D , González María C , Estrada Karel J , Sanchez-Flores Alejandro , Dinkova Tzvetanka D 
Published: July 2015

Abstract
Marine sandy beaches represent dynamic environments often subject to harsh conditions and climate fluctuations, where natural and anthropogenic inputs of freshwater from fluvial and pluvial sources alter salinity, which has been recognized as a key variable affecting the distribution of aquatic organisms and influencing critical physiological processes. The marine arenicolous fungus Corollospora maritima is a worldwide-distributed saprobe that has been reported to present tolerance to freshwater. Here, we present a transcriptome analysis that will provide the first insight of the genomic content for this fungus and a gene expression comparison between two different salinity conditions. We also identified genes that are candidates for being differentially expressed in response to environmental variations on salinity during the fungal growth. The de novo reconstruction of C. maritima transcriptome Illumina sequencing provided a total of 14,530 transcripts (16 megabases). The comparison between the two growth conditions rendered 103 genes specifically overexpressed in seawater, and 132 genes specifically up-regulated under freshwater. Using fungal isolates collected from different beaches, the specific environmental regulation of particular transcript differential expression was confirmed by RT-qPCR. To our knowledge, this is the first analysis that explores the marine fungus C. maritima molecular responses to overcome freshwater stress, and these data could shed light to understand the fungal adaptation and plasticity mechanisms to the marine habitat.
Influence of Mo content on the phase evolution and corrosion behavior of model Fe–9Cr–xMo (x = 5, 7, and 9 wt%) alloys
Authors: Gomes da Silva Marcelo J. , Herculano Luis F.G. , Urcezino Amanda S.C. , Araújo Walney S. , de Abreu Hamilton F.G. , de Lima-Neto Pedro 
Published: July 2015

Abstract
A size-controlled synthesis and characterization of mixed monolayer protected silver-S-(CH2)11-NHCO-coumarin nanoparticles and their Raman activities
Authors: Mlambo Mbuso , Mdluli Phumlane S. , Shumbula Poslet , Skepu Amanda , Tshikhudo Robert , Moloto Nosipho 
Published: July 2015

Abstract
Characterization of ion-induced radiation effects in nuclear materials using synchrotron x-ray techniques
Authors: Lang Maik , Tracy Cameron L. , Palomares Raul I. , Zhang Fuxiang , Severin Daniel , Bender Markus , Trautmann Christina , Park Changyong , Prakapenka Vitali B. , Skuratov Vladimir A. , Ewing Rodney C. 
Published: July 2015

Abstract
Optical and cytotoxicity properties of water soluble type II CdTe/CdSe nanoparticles synthesised via a green method
Authors: Ncapayi Vuyelwa , Oluwafemi Samuel O. , Songca Sandile P. , Kodama Tetsuya 
Published: July 2015

ABSTRACTWe herein report the optical and cytotoxicity properties of highly luminescent water soluble mercaptopropanoic acid (MPA) capped CdTe/CdSe core shell nanoparticles (NPs). The synthesis of the CdTe/CdSe NPs was carried out via a simple, one pot and economical route, involving the use of greener materials under ambient environment in the absence of an inert atmosphere. The temporal evolution of the size and optical properties of the nanomaterials was investigated by varying the reaction time and stability of the as-synthesised material at pH 12. The as-synthesised nanomaterials were characterised using UV-vis absorption and photoluminescence (PL) spectroscopy. The nanoparticles obtained were of high quality with high absorption and emission features. Addition of Se precursor to produce CdSe layer on the CdTe NPs core surface resulted in significant red shirt of both the absorption and emission maxima. The stability study showed that the emission maximum peak positions and FWHM remain the same with increase in emission intensity for all the NPs during the aging period. The cytotoxicity assay showed very high cell viability for the CdTe/CdSe NPs produced at 7 h compared with those produced at 30 mins as the concentration increased from 0.1 to 60 ug/ml. The lower cytotoxicity at the higher reaction time was attributed to the higher stability of the material and hence lower release of Cd2+.
Breast cancer detection using charge sensors coupled to DNA monolayer
Authors: Batistuti Marina R. , Mulato Marcelo , Bueno Paulo R. 
Published: July 2015

ABSTRACTWe report the development of a label-free biosensors based on DNA hybridization, using cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This study uses DNA sequences based on microRNA related with breast cancer. The biosensor was fabricated by immobilizing a self-assembled monolayer of single-stranded 23-mer oligonucleotide (ssDNA) via a thiol linker on gold work electrodes. Residual binding places were filled with 6 -mercaptohexanol (MCH). The electrode was electrochemicaly characterized in the presence of a redox system ferri/ferrocyanide. Different concentrations of complementary DNA sequence for hybridization were incubated; an increase of charge transfer resistance (Rct) was observed, used as sensor parameter and correlated with concentrations of complementary DNA sequence. A debate was presented on the effect of the MgCl2 influence on ssDNA immobilization solution.
Der Tuberkulin-Hauttest: Literatur, Richtlinie und Umsetzung in der Praxis
Authors: Pucken V.-B. , Götz F. , Mansfeld R. , Moder S. , Sauter-Louis C. , Straubinger R. K. , Knubben-Schweizer G. 
Published: July 2015

Zusammenfassung
Gegenstand und Ziel: Aufgrund vermehrter Tuberkuloseausbrüche in Rinderbeständen in der Region Allgäu in den letzten Jahren wurde die flächendeckende Tuberkulinisierung in dieser Region wieder aufgenommen. Ziel dieser Studie war es, einen Überblick über die aktuellen Kenntnisse der Nutztierpraktiker bezüglich der Technik des Tuberkulinisierens zu erhalten sowie basierend auf der aktuellen Gesetzeslage einen Leitfaden zur Anwendung der Methode zu erstellen. Material und Methoden: Das Wissen und die Erfahrungen der Nutztierpraktiker zur aktuellen Tuberkulinisierung wurden mithilfe eines Fragebogens erfragt, gesammelt und ausgewertet. Die Antworten der Tierärzte wurden mit der aktuellen und den vorherigen Fassungen des Anhangs B der Verordnung (EG) Nr. 1226/2002 der Kommission verglichen. Ergebnisse: An der Befragung nahmen insgesamt 137 Tierärzte teil. Sieben Fragebögen waren nicht auswertbar, sodass die Antworten von 130 Tierärzten in die Auswertung eingingen. Vierundvierzig dieser 130 Teilnehmer tuberkulinisierten zum Zeitpunkt der Befragung. Von 44 ausgefüllten Fragebögen der tuberkulinisierenden Teilnehmer konnten 42 in die abschließende Auswertung einfließen. Ein Großteil der Nutztierpraktiker nimmt die Tuberkulinisierung so vor, wie sie in der Verordnung (EG) Nr. 1226/2002 der Kommission vom 8. Juli 2002 zur Änderung von Anhang B der Richtlinie 64/432/EWG des Rates gefordert ist. Bei der Kontrolle der Ergebnisse weichen jedoch viele Praktiker von den Vorgaben in der Verordnung (EG) Nr. 1226/2002 ab. Die Tierärzte, die am stärksten davon abweichen, tuberkulinisieren ent weder nur einzelne Tiere oder nicht im stark betroffenen Regierungsbezirk Schwaben. Schlussfolgerungen: In den besonders von der Tuberkulose betroffenen Gebieten wird die Methode des Tuberkulini sierens von den im Rahmen dieser Studie befragten Tierärzten nahezu so ausgeführt, wie es die Verordnung (EG) Nr. 1226/2002 fordert. Beim Ablesen der Ergebnisse sollte allerdings ein einheitlicheres und sorgsameres Vorgehen angestrebt werden. Der im Rahmen dieser Studie verfasste Leitfaden kann dabei helfen. Die Informationen aus der Literatursichtung zeigen zudem, dass es bisher kein standardisiertes Verfahren zur Tuberkulinisierung gibt.
Age determination in dogs using ocular light reflection, dental abrasion and tartar
Authors: Failing K. , Neiger R. , Gesierich K. 
Published: July 2015

SummaryObjective: The knowledge of an animal’s age is important for disease probability, prognoses, or epidemiological questions, but unfortunately, it is often unknown for dogs in animal shelters. A simple estimating procedure is preferable being quick and easy to perform, even for nonveterinarians. Material and methods: In 295 dogs the dimension of light reflection (diameter in millimetres), visible on the posterior lens capsule using a penlight, the grade of dental abrasion and dental tartar were documented photographically and the exact weight and age in days were obtained. These photographs were evaluated blinded. The dogs were divided randomly into two groups. The first group was used to establish a model for age determination using linear and logistic regression models considering the documented parameters, which was then validated with the data of the second group. Results: The size of ocular light reflection and age correlated significantly (r = 0.781; p < 0.001; sy,x = 2.45 years [SD of y for given x]). The linear regression model gave the final equation: Estimated age [months] = 13.954 + 33.400 × lens reflection [mm] + 8.406 × dental abrasion [grade] + 8.871 × tartar [grade] with a standard error of estimation of 2.26 years. Conclusion and clinical relevance: Age determination, even based on three parameters results in a large standard deviation making age estimation in dogs very crude.
Music, Violence and Music Therapy with Young People in Schools: A Position Paper
Authors: McFerran Katrina Skewes , Wölfl Andreas 
Published: July 2015

Music therapists have rarely involved themselves in the discourse linking music and violence. Instead, representatives of the profession have advocated for the positive outcomes that can result from the use of music by trained therapists working with people who have experienced violence or been violent. In this position paper, we will elaborate a much-needed position that first acknowledges the ways that music can promote violence, and then focuses on different ways to work with young people involved in violence. The position paper is structured around the examination of three types of music therapy programs and our purpose is to bring to light the beliefs that underpin each program, both the viewpoints about the cause of violence, and assumptions about how potentially negative relationships between young people and music can be made positive. We conclude by describing different methods for evaluating such programs that are congruent with the assumptions that underpin them. It is hoped that the positions we delineate will support music therapists and others to be more conscious of the relationship between music and violence when designing programs for young people.
Ten years on (some lessons from the transition)
Authors: Tošovský Josef 
Published: July 2015

The article offers essential reflections on ten years of experience with the transformation of the former centrally planned economies into free market economies. Although there were large differences among individual transition countries, all of them faced similar challenges early in the 1990s. The unique initial conditions of the Central and Eastern European countries called for an approach fundamentally different from reforms applied in Latin America or Asia. CEE countries had to rebuild, from scratch, a private sector, create a market-compatible institutional environment, and correct the serious inherited deformations of the real economy. In addition, at the same time they were confronted with the international liberalization of flows of goods and capital. The article aims to distinguish and analyse both objective and subjective factors affecting the process of economic transformation.
Transformation, integration and inequality: the case of the czech republic
Authors: Sojka Milan 
Published: July 2015

The paper concentrates on the criticism of the Czech neo-liberal transformation strategy resulting from its implementation. It brings about an analysis of major factors leading to adverse effects on the restructuring of former big state-owned enterprises, on the structure of Czech exports (due to its stress on the "traditional" industrial comparative advantages and especially cheap labor), and on the development of earnings distribution from the point of view of a "meritocratic ideal". This analysis of earnings distribution and its development during the 1990s shows an important role plaid by factors that have lead to creating suitable conditions for predatory and speculative patterns of behavior. This analysis serves as a basis for supporting an alternative modernization strategy of transformation of former Soviet-type economies and societies.
Clinical disease associated with epizootic hemorrhagic disease virus in cattle in Illinois
Authors: Garrett Edgar F. , Po Eleonora , Bichi Elena R. , Hexum Suzette K. , Melcher Robert , Hubner Andrew M. 
Published: July 2015

Abstract
Objective—To describe the clinical manifestation of epizootic hemorrhagic disease virus (EHDV) in cattle during an outbreak in northwestern Illinois in the fall of 2013.
Design—Outbreak survey.
Sample—18 EHDV-affected cattle herds.
Procedures—Herds with confirmed EHDV-infected cattle were selected for the survey on the basis of having a manager willing to participate. A survey was developed and administered to obtain information regarding the demographics of EHDV-affected herds and the clinical signs and outcomes for EHDV-infected cattle.
Results—The managers of 13 beef and 5 dairy herds participated in the survey. The herds contained approximately 1,400 cattle, of which 61 were infected with EHDV and 16 died. Most cattle clinically affected with EHDV were adults, although 1 herd had 6 calves with clinical signs, and EHDV was identified during postmortem testing of 1 of those calves. Clinical signs most commonly observed were oral ulcerations or erosions, anorexia, weight loss, and lameness that typically lasted > 7 days. Of the 18 herds, 17 had wooded areas and 14 had at least 1 pond or marsh on the property. Deer were observed on the property of all farms. The EHDV outbreak in cattle coincided with an EHDV outbreak in deer in the region.
Conclusions and Clinical Relevance—This large-scale outbreak of EHDV in US cattle was unusual because calves were affected. Because there is no way to effectively predict or prevent an EHDV outbreak in cattle, development of a vaccine for use in cattle during an outbreak would be beneficial. (J Am Vet Med Assoc 2015;247:190–195)
MHC class I-presented tumor antigen appraisable for T-cell responses against ovarian cancer
Authors: Wang Jing Yao , Zhang Nan , Yang Xiaojie , Gao Danli , Yin Lirong , Ji Shuying 
Published: July 2015

<p>The purpose of this study is to assess whether MHC class I-presented tumor antigen is appraisable for T-cell responses against ovarian cancer. In ovarian cancer cell, human leukocyte antigen A2 (HLA-A2) associated with peptides was used to promote the activation of naive T cells so as to activate antigen-specific T cells. 7 or 4 patients were observed grade 1 or 2 injection site reactions, respectively. 5, 2 or 1 patients were observed grade 1, 2 or 3 pain reactions, respectively. 4 or 1 patients were observed grade 1 or 2 induration reactions. Total number mean value of patients experiencing response to the particular peptide was 7.73, and total number mean value of peptides to which the patients responded was 7.45. MHC class I-presented tumor antigen is appraisable for T-cell responses against ovarian cancer in China.</p>
Synthesis and biological evaluation of new hydrazide-Schiff bases
Authors: Husain Asif , Varshney Munendra M. , Parcha Versha , Ahmad Aftab , Khan Shah Alam 
Published: July 2015

<div><p>A new series of N-({5-(substituted aryl)-furan-2-yl}-methylidene)-hydrazides were synthesized with a new class of Schiff bases derived from the reaction of substituted phenyl-1-ketohydrazide <strong>2</strong> or 2-(4-chloro-3-methylaryloxy) acetohydrazide <strong>3</strong> with different 5-(substituted aryl)-2-furfuraldehyde (<strong>1a-k</strong>) to yield substituted N-({5-(substituted aryl)-furan-2-yl}-methylidene)-hydrazides-Schiff bases <strong>(4a-f, 4g-k)</strong>. The title compounds were subjected to <em>in vitro</em> antibacterial screening against Gram positive bacterial strains-<em> S. aureus</em>, <em>B. cereus</em>, <em>E. faecalis</em> and <em>S. epidermidis</em>, and Gram negative bacteria strains- <em>E. coli</em>, <em>S. typhi</em>, <em>S. dysenteriae</em> and <em>K. pneumoniae</em>. The synthesized Schiff bases were also evaluated for their anthelmintic activity against two species of earthworms (<em>Pheritima posthuma</em> and <em>Perionyx excavates</em>). Some compounds have shown promising antibacterial and anthelmintic activities.</p></div>