bluuebunny/crossref_metadata_2025_split huggingface dataset:
Annexin A5 promotes macrophage activation and contributes to pulmonary fibrosis induced by silica particles
Authors: Luo C , Ji X , Fan J , Hou Z , Wang T , Wu B , Ni C 
Published: March 2015

Objective: To investigate the contributions and underlying molecular mechanisms of annexin A5 toward silica-induced pulmonary fibrosis. Methods: Male C57BL/6 mice were randomly divided into three groups and instilled intratracheally with silica, saline, or air. Mice were euthanized at 3, 7, 14, or 28 days following treatment. Annexin A5 levels in serum and lung tissues were detected by enzyme-linked immunosorbant assay (ELISA) assays or Western blots. The association of annexin A5 levels with silica-induced lung fibrosis was further investigated in the macrophage cell line, RAW264.7. Following exposure of these cells to silica at a concentration of 200 μg/ml for 6 or 12 h, the expression levels of transforming growth factor β1 (TGF-β1), interleukin 1α (IL-1α), Fas ligand (FasL), and their downstream targets were evaluated by Western blots. Furthermore, annexin A5 and FasL were knocked down by small interfering ribonucleic acid (siRNA) and TGF-β1 secretion into the cell culture medium was measured by ELISA assays or Western blots. Results: Mice treated with silica demonstrated lung fibrosis at 28 days following exposure, whereas, in controls, only mild and transient inflammation was evident at day 3 and day 7 postinstillation and was not present at day 14. Furthermore, silica-exposed mice exhibited significantly ( p < 0.05) elevated levels of annexin A5 in serum and lung tissues, relative to control groups. Consistent with these findings, silica exposure of RAW264.7 cells for 6 or 12 h, led to an annexin A5-dependent increase in the expression levels of TGF-β1, IL-1α, FasL, and their downstream target molecules. These silica-induced changes were reversed by siRNA-mediated knockdown of annexin A5, but downregulation of FasL led to increased annexin A5 expression and reduced levels of TGF-β1, IL-1α, and FasL downstream target molecules. Conclusions: These findings define a role of annexin A5 in promoting macrophage activation via Fas/FasL pathways in silica-induced lung fibrosis.
Functional Outcomes of Tibialis Posterior Tendoscopy With Comparison to Magnetic Resonance Imaging
Authors: Gianakos Arianna L. , Ross Keir A. , Hannon Charles P. , Duke Gavin L. , Prado Marcelo P. , Kennedy John G. 
Published: March 2015

Background: The purpose of the current study was to report functional outcomes of tendoscopy for treatment of tibialis posterior tendon pathology as well as compare its diagnostic capability with magnetic resonance imaging (MRI). Methods: Clinical records and MRI of 12 patients who underwent tendoscopy of the tibialis posterior tendon (TPT) were retrospectively reviewed. Mean follow-up was 31 months (range, 26-43 months). Preoperative MRI findings were compared with tendoscopic findings to assess the diagnostic agreement between each modality. Functional outcomes were assessed using the Foot and Ankle Outcome Score (FAOS) and Short Form-12 (SF-12) General Health Questionnaire pre- and postoperatively. Mean patient age was 43 years (range, 17-63 years). Mean duration of preoperative symptoms was 15.5 months (range, 3-36 months). Results: Pathologies addressed via tendoscopy included tenosynovitis, tendinosis, stenosis, tendon subluxation, and partial thickness tear (via mini-arthrotomy). Preoperative MRI findings were in agreement with tendoscopic findings in 8 of 12 cases (67%). Tendoscopy diagnosed and allowed access for treating pathology that was missed on MRI in the remaining four cases. The FAOS improved from a mean preoperative score of 58 (range, 36-78) to a mean postoperative score of 81 (range, 44-98) ( P < .01). The SF-12 score improved from a mean preoperative score of 34 (range, 13-51) to a mean postoperative score of 51 (range, 21-76) ( P = .01). Conclusions: Although MRI is considered an effective imaging technique for tendon pathology, tendoscopy may be a more sensitive diagnostic tool. Tendoscopy was an effective minimally invasive tool to diagnose and treat tibialis posterior tendon pathology resulting in functional improvements in the short-term for early stage TPT dysfunction. Further studies comparing tendoscopy with traditional open approaches are warranted. Level of Evidence: Level IV case series.
Cryosurgery in association with itraconazole for the treatment of feline sporotrichosis
Authors: de Souza Clarissa Pimentel , Lucas Ronaldo , Ramadinha Regina HR , Pires Tifanny BCP 
Published: March 2015

Objectives We evaluated the efficacy of cryosurgery in association with itraconazole for the treatment of feline sporotrichosis. We also compared the length of treatment protocol with others reported in the literature. Methods Cats naturally infected with fungi of the Sporothrix schenckii complex were evaluated. Diagnosis was confirmed by cytology and fungal culture. Prior to the cryosurgical procedure, every animal was receiving itraconazole 10 mg/kg/day PO, for different time periods. The same protocol was maintained until 4 weeks after complete healing of the lesions. Results Eleven of 13 cats were considered clinically cured. The treatment duration ranged from 14–64 weeks (median 32 weeks). Conclusions and relevance The combination of cryosurgery and itraconazole was effective in treating cases of feline sporotrichosis and decreased the treatment length compared with protocols using only medication.
N-3-(oxododecanoyl)-L-homoserine lactone promotes the induction of regulatory T-cells by preventing human dendritic cell maturation
Authors: Li Youqiang , Zhou Huayou , Zhang Yunyan , Chen Cha , Huang Bin , Qu Pinghua , Zeng Jianming , E Shunmei , Zhang Xuan , Liu Jianping 
Published: March 2015

N-3-(Oxododecanoyl)-L-homoserine lactone (C12) is a small bacterial signaling molecule secreted by Pseudomonas aeruginosa (PA), which activates mammalian cells through TLR4-independent mechanisms. C12 acts as an immunosuppressant and it has been shown to modulate murine bone marrow-derived dendritic cell-mediated T-helper 2 (Th2) cell polarizations in vitro. In the present study, we initially examined the impact of C12 on the maturation of human monocyte-derived dendritic cells (Mo-DCs) and the induction of regulatory T-cells (iTregs) in culture. Our findings demonstrate that C12-treated Mo-DCs failed to undergo lipopolysaccharide (LPS)-induced maturation. At the molecular level, C12 blocked the upregulation of surface molecules, including CD11c, HLA-DR, CD40, and CD80, and it switched to an interleukin (IL)-10high, IL-12p70low phenotype. Moreover, C12 selectively inhibited the capacity of Mo-DCs to stimulate the proliferation of allogeneic CD4+ T-cells. Otherwise, the C12-treated Mo-DCs promoted the generation of CD4+CD25+Foxp3+-induced regulatory T-cells (iTregs) and enhanced their IL-10 and transforming growth factor (TGF)-β production associated with reduced interferon (IFN)-γ and IL-12p70 production. These findings provide new insights towards understanding the persistence of chronic inflammation in PA infection.
Palmar arch anatomy: Ajmer Working Group classification
Authors: Gokhroo Rajendra , Bisht Devendra , Gupta Sajal , Kishor Kamal , Ranwa Bhanwar 
Published: March 2015

Introduction Forearm arteries are frequently used as workhorse site for cardiac catheterization, bypass grafting and haemodialysis access. There is paucity of data on palmar circulation in live human being and only cadaveric data are available till date. We, therefore, made an attempt to look at the various patterns of sufficient or insufficient palmar arch circulation and various anomalies of forearm arteries, to discuss their clinical implications. Methods and results We obtained the forearm and hand arteriograms of patients (n = 302) through radial (n = 200) and ulnar routes (n = 102). Modified Allen’s test was normal in all of our patients. On the basis of predetermined parameters angiograms were analysed and findings were divided into three groups. These three groups were further classified as type A, type B, type C superficial palmar arch. Conclusion We concluded that type A superficial palmar arch is most suitable for providing adequate collateral circulation in case of harvesting of forearm vessel, whereas type C superficial palmar arch appears to be highly susceptible for digital ischemia in case of radial or ulnar artery occlusion. Modified Allen’s test alone is not justifiable for documenting good collateral circulation and it should be supplemented by other tests to document good collateral circulation before proceeding to any radical procedure.
Translating What Works
Authors: Phillips Lawrence S. , Barb Diana , Yong Chun , Tomolo Anne M. , Jackson Sandra L. , Olson Darin E. , Rhee Mary K. , Duva Ingrid M. , He Qing , Long Qi 
Published: March 2015

Background: The most efficacious strategies to improve diabetes control include case management, health care team changes, patient education, and facilitated transmission of patient data to clinicians (“facilitated relay”), but these strategies have not been translated to permit general use in clinical practice. Methods: A web-based decision support program was developed to include these features, and assessed in patients who had A1c ≥7.0% despite using metformin with/without sulfonylureas or insulin. Staff entered patients’ glucose data, obtained management recommendations, reviewed the plan with a clinician, and discussed the new plan with patients. Results: 113 subjects were 96% male and 32% black, with average age 65.6 years and BMI 32.8. During prior primary care, A1c averaged 8.32 ± 0.16% (SEM). In all patients, baseline A1c was 8.18 ± 0.11%, and decreased to 7.54 ± 0.12%, 7.16 ± 0.13%, and 7.54 ± 0.16% at 3, 6, and 12 months, respectively, all P < .001. In 42 subjects who provided glucose data and made requested changes in medications, A1c was 8.12 ± 0.09% at baseline and fell to 7.29 ± 0.11%, 6.98 ± 0.10%, and 7.05 ± 0.10% at 3, 6, and 12 months, respectively, all P < .001. Chart review of 16 subjects followed for 12 months demonstrated that hypoglycemia (symptoms and/or glucose <70 mg/dl) averaged less than 1 episode/patient/month, and there was no severe hypoglycemia. Conclusions: A novel decision support program improved A1c with little hypoglycemia. Use of this approach should allow primary care teams to keep patients well controlled, and reduce the need for specialist referrals.
Patient-Reported Outcomes and Their Predictors at Minimum 10 Years After Anterior Cruciate Ligament Reconstruction
Authors: Magnussen Robert A. , Verlage Megan , Flanigan David C. , Kaeding Christopher C. , Spindler Kurt P. 
Published: March 2015

Background: Long-term results after anterior cruciate ligament (ACL) reconstruction are being reported with greater frequency, allowing a detailed analysis of long-term patient-reported outcomes and predictors. Purpose: To summarize expected patient-reported outcomes at a minimum 10 years following ACL reconstruction and to explore patient and surgical factors that affect these results. Study Design: Systematic review; Level of evidence, 4. Methods: Prospective studies detailing patient-reported outcomes with a minimum follow-up of 10 years were identified. Average scores for each outcome measure were calculated. Factors identified in each paper as predictors of patient-reported outcomes were identified and described. Results: Thirteen studies met inclusion and exclusion criteria. Lysholm scores were reported in 317 of 406 patients (78.1%) in 6 studies, with a mean score of 91.7 ± 11.2. Subjective International Knee Documentation Committee scores were reported in 1726 of 2611 patients (66.1%) in 5 studies, with a mean score of 84.2 ± 15.5. Cincinnati knee scores were reported in 1323 of 1801 patients (73.5%) in 3 studies, with a mean score of 87.4 ± 14.4. Tegner activity scores were reported in 728 of 914 patients (79.6%) in 8 studies, with a mean score of 5.1. There was mixed evidence that meniscectomy and articular cartilage damage were associated with poorer patient-reported outcomes. Patient sex and graft choice did not affect patient-reported outcomes. Conclusion: Patient-reported outcomes are generally good at a minimum of 10 tears following ACL reconstruction. Further large prospective studies with regression modeling and consistent outcome reporting will clarify predictors of outcomes.
Prognostic value of FDG-PET prior to autologous stem cell transplantation for relapsed and refractory diffuse large B-cell lymphoma
Authors: Sauter Craig S. , Matasar Matthew J. , Meikle Jessica , Schoder Heiko , Ulaner Gary A. , Migliacci Jocelyn C. , Hilden Patrick , Devlin Sean M. , Zelenetz Andrew D. , Moskowitz Craig H. 
Published: March 2015

Key Points
FDG-PET–assessed response to ST according to Deauville criteria predicts outcome post-ASCT for rel/ref DLBCL.
Hematopoietic stem cells: concepts, definitions, and the new reality
Authors: Eaves Connie J. 
Published: March 2015

Abstract
Hematopoietic stem cell (HSC) research took hold in the 1950s with the demonstration that intravenously injected bone marrow cells can rescue irradiated mice from lethality by reestablishing blood cell production. Attempts to quantify the cells responsible led to the discovery of serially transplantable, donor-derived, macroscopic, multilineage colonies detectable on the spleen surface 1 to 2 weeks posttransplant. The concept of self-renewing multipotent HSCs was born, but accompanied by perplexing evidence of great variability in the outcomes of HSC self-renewal divisions. The next 60 years saw an explosion in the development and use of more refined tools for assessing the behavior of prospectively purified subsets of hematopoietic cells with blood cell–producing capacity. These developments have led to the formulation of increasingly complex hierarchical models of hematopoiesis and a growing list of intrinsic and extrinsic elements that regulate HSC cycling status, viability, self-renewal, and lineage outputs. More recent examination of these properties in individual, highly purified HSCs and analyses of their perpetuation in clonally generated progeny HSCs have now provided definitive evidence of linearly transmitted heterogeneity in HSC states. These results anticipate the need and use of emerging new technologies to establish models that will accommodate such pluralistic features of HSCs and their control mechanisms.
Planning for the future workforce in hematology research
Authors: Hoots W. Keith , Abkowitz Janis L. , Coller Barry S. , DiMichele Donna M. 
Published: March 2015

AbstractThe medical research and training enterprise in the United States is complex in both its scope and implementation. Accordingly, adaptations to the associated workforce needs present particular challenges. This is particularly true for maintaining or expanding national needs for physician-scientists where training resource requirements and competitive transitional milestones are substantial. For the individual, these phenomena can produce financial burden, prolong the career trajectory, and significantly influence career pathways. Hence, when national data suggest that future medical research needs in a scientific area may be met in a less than optimal manner, strategies to expand research and training capacity must follow. This article defines such an exigency for research and training in nonneoplastic hematology and presents potential strategies for addressing these critical workforce needs. The considerations presented herein reflect a summary of the discussions presented at 2 workshops cosponsored by the National Heart, Lung, and Blood Institute and the American Society of Hematology.
Controllable photoluminescence enhancement of CdTe/CdS quantum dots thin films incorporation with Au nanoparticles
Authors: Wang Hongyu , Xu Ling , Zhang Renqi , Ge Zhaoyun , Zhang Wenping , Xu Jun , Ma Zhongyuan , Chen Kunji 
Published: March 2015

Abstract
Au nanoparticles (Au NPs)/CdTe/CdS QDs nanocomposite films were fabricated by deposition of Au NPs and layer-by-layer self-assembly of colloidal CdTe/CdS QDs. Photoluminescence (PL) spectra showed that Au NPs incorporation resulted in an increase of PL intensity about 16-fold compared with that of the samples without Au NPs. PL enhancement of Au NPs/CdTe/CdS QDs nanocomposite films can be controlled by tuning the thickness of spacer layer between the metal nanoparticles (MNPs) and QDs. Optical absorption spectra exhibited the incorporation of Au NPs boosted the absorption of Au NPs/CdTe/CdS QDs nanocomposite films. The results of finite-difference time-domain (FDTD) simulation indicated that the increased sizes of Au NPs resulted in stronger localization of electric field, which boosted the PL intensity of QDs in the vicinity of Au NPs. We thought that these were mainly attributed to localized SP enhancement effects of the Au NPs. Our experiment results demonstrated that Au NPs/QDs nanocomposite films would be a promising candidate for optoelectronic devices application.

PACS

78.55.-m; 82.33.Ln; 68.65.Hb
Ultradeep analysis of tumor heterogeneity in regions of somatic hypermutation
Authors: Spence Janice M , Spence John P , Abumoussa Andrew , Burack W Richard 
Published: March 2015

AbstractTumor heterogeneity is of growing importance in the treatment of cancers. Mutational hot spots are prime locations for determining number and proportions of low variant allele frequency (VAF) tumor subclones by next generation sequencing. Low VAF detection is complicated by poor mapping efficiency in regions with high mutation density. Our Deep-Drilling with iterative Mapping (DDiMAP) method retains variant allele patterns to aid in single nucleotide variation detection and generation of additional reference alleles, with remapping increasing coverage of highly mutated regions to capture data critical to heterogeneity analysis and enhancing sensitivity. DDiMAP outputs variant patterns with frequencies, enabling rapid phylogenetic analysis of ongoing mutation.
HIF-1A and C/EBPs transcriptionally regulate adipogenic differentiation of bone marrow-derived MSCs in hypoxia
Authors: Jiang Chen , Sun Jun , Dai Yafei , Cao Pengfei , Zhang Liyang , Peng Shuping , Zhou Yanhong , Li Guiyuan , Tang Jingqun , Xiang Juanjuan 
Published: March 2015

Abstract
Introduction
Bone marrow-derived mesenchymal stem cells (BMSCs, also known as bone marrow-derived mesenchymal stromal cells) are known to be a component of the tumor microenvironment. BMSCs are multipotent stromal cells that can differentiate into a variety of cell types, including osteocytes, chondrocytes, adipocytes, epithelial cells and endothelial cells. Stem cells found in niches or transplanted into injured tissues constantly encounter hypoxic stress. Areas with very low to no oxygen pressure exist in solid tumors. The differentiation capacity of BMSCs under hypoxic conditions remains controversial.

Methods
In this study, a hypoxic workstation, set at an oxygen concentration of 0.2% was used to mimic the hypoxic microenvironment of cancer in vivo. Oil red O staining and alkaline phosphatase staining were used to examine the adipogenic or osteogenic differentiation, respectively, of BMSCs. Real-time PCR was performed to explore the expression of adipocyte- or osteocyte-specific genes. An RT2 Profiler™ PCR Array was used to screen a panel of 84 genes associated with human adipogenesis in BMSCs under normal and hypoxic conditions. A dual-luciferase reporter assay and chromatin immunoprecipitation (ChIP) were applied to analyze promoter activity to evaluate the possible regulatory mechanism of adipocyte-specific gene expression.

Results
We found that this extreme hypoxia impaired osteogenic differentiation as indicated by the attenuation of alkaline phosphatase (ALP) activity and the reduced expression of osteogenic markers osteocalcin and osteopontin. Moreover, extreme hypoxia enhanced adipogenic differentiation, as indicated by the accumulation of lipid droplets and the expression of the adipocyte-specific genes leptin, LPL, CFD, PGAR and HIG2. In the extreme hypoxic conditions (0.2% oxygen), the overexpression of CCAAT enhancer-binding proteins (C/EBPs), especially C/EBPδ, and HIF-1A upregulated the promoter activities of adipocyte-specific genes such as leptin, CFD, HIG2, LPL, PGAR. In the present study, peroxisome proliferator-activated receptor-gamma (PPARγ) exerted a negative effect on the differentiation of BMSCs into adipocytes.

Conclusions
In view of these findings, extreme hypoxia induced the adipogenic differentiation of BMSCs through HIF-1A and C/EBPs. These findings might provide clues regarding the roles of BMSCs in the cancer microenvironment.
Role of the Amide Carbonyl Groups in the Nitrile Hydratase Active Site for Nitrile Coordination Using Co(III) Complex with N2S3-type Ligand
Authors: Yano Takuma , Ikeda Tomohiro , Shibayama Tomonori , Inomata Tomohiko , Funahashi Yasuhiro , Ozawa Tomohiro , Masuda Hideki 
Published: March 2015

Abstract
The role of the amide carbonyl oxygens in the nitrile hydratase (NHase) active site for the nitrile coordination to the metal center was studied using a distorted square-pyramidal N2S3-type Co(III) complex at room temperature in the presence of a folded-sheet mesoporous material (FSM) or sodium cation.
Le séjour du poète grec André Calvos à Genève et Lausanne
Authors: Bouvier-Bron Michelle 
Published: March 2015

<p>L'objet de cet article était de recenser les renseignements concernant le poète grec André Calvos pendant son long séjour à Genève (mai 1821-décembre 1824) dans les Archives d'État (conditions de vie); dans les journaux genevois de l'époque (annonce de la publication de son premier recueil d'odes <em>La Lyre</em>, de celle des cours de littérature italienne, puis grecque qu'il se propose de donner); dans les registres de la Société de Lecture (contacts probables avec l'helléniste Élie-Ami Bétant, l'homme politique James Fazy et le comte Jean Capodistrias). Nous nous sommes interrogée sur les relations qu'il a pu avoir avec les réfugiés piémontais et lombards arrivés nombreux à Genève en 1821 et 1822, et surtout avec le célèbre révolutionnaire Filippo Buonarroti. Enfin, nous donnons une transcription diplomatique de la lettre de recommandation en faveur de Calvos que Jean Capodistrias, alors installé à Genève, adresse le 8 novembre 1824 à Lausanne à son ami Frédéric-César de La Harpe, ancien précepteur du tsar Alexandre et, à l'époque de Calvos, personnage hautement contestataire, proche de la Charbonnerie.</p><p> </p><p> </p>
The Influence of Darwinian Ideas on Greek Literary Writers of the Late Nineteenth and Early Twentieth Centuries: The Case of Emmanuel Roidis
Authors: Zarimis Maria 
Published: March 2015

<p>Darwin's works provoked an enormous response in many disciplines including the literary world. This paper presents a portion of my doctoral thesis3, which responds to a blind spot in Greek literary scholarship on evolutionary ideas in comparison to other Western countries. Little work to date focuses on modern Greek writers's responses to Darwinian and other evolutionary ideas. This paper explores the impact of Darwin in selected writings of Emmanuel Roidis and how Roidis satirised Darwinism in his essays and short stories, contributing to the Darwinian discourse on "man's place in nature" and by placing humanity on the same continuum as other primates. The year 2009 marks the 200th anniversary of Darwin's birth and the 150th anniversary of the first publication of his <em>The Origin of Species</em>. It is timely, then, to consider Darwin's impact on modern Greek literature.</p><p> </p><p> </p>