bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete Genome Sequence of Solvent-Tolerant Clostridium beijerinckii Strain SA-1
Authors: Noar Jesse , Makwana S. T. , Bruno-Bárcena José M. 
Published: December 2014

ABSTRACT

We report the complete genome sequence of
Clostridium beijerinckii
SA-1, derived by directed evolution from
C. beijerinckii
NCIMB 8052, selecting for enhanced solvent tolerance. This sequence allows for accurate placement of SA-1 as
C. beijerinckii
, permits functional analyses of mutant phenotypes, and suggests methods for distinguishing SA-1 from its parent.
Draft Genome Sequence of Chelonobacter oris Strain 1662
T
, Associated with Respiratory Disease in Hermann's Tortoises
Authors: Kudirkiene Egle , Hansen Mie Johanne , Bojesen Anders Miki 
Published: December 2014

ABSTRACT

Chelonobacter oris
1662
T
is a type strain of the recently described species of the
Pasteurellaceae
family. The strain was isolated from the choanae of a captive tortoise with signs of respiratory tract infection. The genome reported here is approximately 2.6 Mb in size and has a G+C content of 47.1%.
Immediate Effect of Three Different Electroacupuncture Protocols on Fasting Blood Glucose in Obese Patients: A Pilot Study
Authors: Belivani Maria , Lundeberg Thomas , Cummings Mike , Dimitroula Charikleia , Belivani Nicole , Vasilakos Dimitris , Hatzitolios Apostolos 
Published: December 2014

Background Obesity is an increasing global health problem, and current methods of management are limited. Preliminary research data suggest that acupuncture may have an influence on metabolic parameters related to obesity. Objective To determine the electroacupuncture (EA) protocol to be used in a future clinical trial examining the effect of acupuncture on metabolic parameters related to obesity and to examine whether a single EA treatment can change fasting blood glucose in obese subjects. Methods 16 obese women aged 30–52 years with body mass index >30 kg/m2 were assigned consecutively into three groups and their fasting blood glucose was measured before and after administering a single session, lasting 30 min, of one of three EA treatment protocols. The Dorsal group received EA to dorsal segmental acupuncture points BL18–23 bilaterally (corresponding to the segmental levels innervating the pancreas); the Ear group received EA to ear points in the cavum conchae; and the Limb group received EA to points in the arms and legs (LI10–LI11, ST36– Zongping). Results After a single session of EA there was a statistically significant decrease in fasting blood glucose in the Dorsal and Limb groups, but there was no change and even a trend towards an increase in the glucose level in the Ear group. Conclusions The findings of this small pilot study suggest that EA to either dorsal segmental points corresponding to the pancreas or to muscle points in all four limbs may exert a beneficial effect on glucose metabolism in obese women.
The late rifting phase and continental break-up of the southern South Atlantic: the mode and timing of volcanic rifting and formation of earliest oceanic crust
Authors: Koopmann H. , Schreckenberger B. , Franke D. , Becker K. , Schnabel M. 
Published: December 2014

Abstract

Multichannel seismic and potential field data shed light on the final rifting stage in the southern South Atlantic. This was associated with major episodes of magmatism during the Early Cretaceous continental break-up. An asymmetrical simple shear-dominated variable strain rifting model is proposed with the margin asymmetry visible in shelf width, amplitude of magnetic anomalies, orientation of break-up-related sedimentary basins and basement slope angle. Along-margin rotation in spreading- and later rifting-direction from north–south to west–east are of great importance for the asymmetries. Such rotational opening may also explain why the southernmost segments of the South Atlantic are magma starved, with a sharp transition to a volcanic-rifted margin type northwards. Interpretation of pre-M5 (
c.
130 Ma) magnetic seafloor spreading lineations constrains the timing of excess break-up-related volcanism and transition to ‘normal’ seafloor spreading. Termination of magnetic anomalies within seaward-dipping reflector sequences points towards a deposition of the volcanics from south to north prior to and during the early rift and opening stages. Identification of previously unknown pre-M5 magnetic lineations offshore Argentina completes the lineation pattern in the southern South Atlantic. The oldest magnetic anomaly related to oceanic spreading is M9 (
c.
135 Ma). Older anomalies, previously identified as M11 (
c.
137 Ma) offshore Cape Town, are related to structural or magnetization variations within seaward-dipping reflector sequences.
Translational Impact of Nanoparticle–Drug Conjugate CRLX101 with or without Bevacizumab in Advanced Ovarian Cancer
Authors: Pham Elizabeth , Birrer Michael J. , Eliasof Scott , Garmey Edward G. , Lazarus Douglas , Lee Christina R. , Man Shan , Matulonis Ursula A. , Peters Christian G. , Xu Ping , Krasner Carolyn , Kerbel Robert S. 
Published: December 2014

Abstract
Purpose: Increased tumor hypoxia and hence elevated hypoxia-inducible factor-1α (HIF1α) is thought to limit the efficacy of vascular endothelial growth factor (VEGF) pathway–targeting drugs by upregulating adaptive resistance genes. One strategy to counteract this is to combine antiangiogenic drugs with agents able to suppress HIF1α. One such possibility is the investigational drug CRLX101, a nanoparticle–drug conjugate (NDC) containing the payload camptothecin, a known topoisomerase-I poison.
Experimental Design: CRLX101 was evaluated both as a monotherapy and combination with bevacizumab in a preclinical mouse model of advanced metastatic ovarian cancer. These preclinical studies contributed to the rationale for undertaking a phase II clinical study to evaluate CRLX101 monotherapy in patients with advanced platinum-resistant ovarian cancer.
Results: Preclinically, CRLX101 is highly efficacious as a monotherapy when administered at maximum-tolerated doses. Furthermore, chronic low-dose CRLX101 with bevacizumab reduced bevacizumab-induced HIF1α upregulation and resulted in synergistic efficacy, with minimal toxicity in mice. In parallel, initial data reported here from an ongoing phase II clinical study of CRLX101 monotherapy shows measurable tumor reductions in 74% of patients and a 16% RECIST response rate to date.
Conclusions: Given these preclinical and initial clinical results, further clinical studies are currently evaluating CRLX101 in combination with bevacizumab in ovarian cancer and warrant the evaluation of this therapy combination in other cancer types where HIF1α is implicated in pathogenesis, as it may potentially be able to improve the efficacy of antiangiogenic drugs. Clin Cancer Res; 21(4); 808–18. ©2014 AACR.
Angiotensin II Mobilizes Spleen Monocytes to Promote the Development of Abdominal Aortic Aneurysm in
Apoe
−/−
Mice
Authors: Mellak Safa , Ait-Oufella Hafid , Esposito Bruno , Loyer Xavier , Poirier Maxime , Tedder Thomas F. , Tedgui Alain , Mallat Ziad , Potteaux Stéphane 
Published: December 2014

Objective—
Abdominal aortic aneurysm (AAA) is widespread among elderly people and results in progressive expansion and rupture of the aorta with high mortality. Macrophages, which are the main population observed within the site of aneurysm, are thought to derive from circulating monocytes although no direct evidence has been provided to date. In this study, we were particularly interested in understanding the trafficking behavior of monocyte subsets in AAA and their role in disease pathogenesis.

Approach and Results—

Using bone marrow transplantation in
Apoe
−/−
mice, we showed that circulating monocytes give rise to abdominal aortic macrophages in hypercholesterolemic mice submitted to angiotensin II (AngII). Detailed monitoring of monocyte compartmentalization revealed that lymphocyte antigen 6C
high
and lymphocyte antigen 6C
low
monocytes transiently increase in blood early after AngII infusion and differentially infiltrate the abdominal aorta. The splenic reservoir accounted for the mobilization of the 2 monocyte subsets after 3 days of AngII infusion. Spleen removal or lymphocyte deficiency in
Apoe
−/−
Rag2
−/−
mice similarly impaired early monocyte increase in blood in response to AngII and protected against AAA development, independently of blood pressure. Reconstitution of
Apoe
−/−
Rag2
−/−
mice with total splenocytes but not with B-cell–depleted splenocytes restored monocyte mobilization in response to AngII and enhanced susceptibility to AAA.

Conclusions—

Taken together, the data show that lymphocyte antigen 6C
high
and lymphocyte antigen 6C
low
monocytes are mobilized from the spleen in response to AngII. Intriguingly, the process is dependent on the presence of B cells and significantly contributes to the development of AAA and the occurrence of aortic rupture.
Association of Postural Instability With Asymptomatic Cerebrovascular Damage and Cognitive Decline
Authors: Tabara Yasuharu , Okada Yoko , Ohara Maya , Uetani Eri , Kido Tomoko , Ochi Namiko , Nagai Tokihisa , Igase Michiya , Miki Tetsuro , Matsuda Fumihiko , Kohara Katsuhiko 
Published: December 2014

Background and Purpose—
Asymptomatic cerebral small-vessel disease (cSVD) in elderly individuals are potent risk factors for stroke. In addition to common clinical risk factors, postural instability has been postulated to be associated with cSVD in older frail patients. Here, we conducted a cross-sectional study to understand the possible link between postural instability and asymptomatic cSVD further, namely periventricular hyperintensity, lacunar infarction, and microbleeds, as well as cognitive function, in a middle-aged to elderly general population (n=1387).

Methods—
Postural instability was assessed based on one-leg standing time (OLST) and posturography findings. cSVD was evaluated by brain magnetic resonance imaging. Mild cognitive impairment was assessed using a computer-based questionnaire, and carotid intima-media thickness as an index of atherosclerosis was measured via ultrasonography.

Results—

Frequency of short OLST, in particular <20 s, increased linearly with severity of cSVD (lacunar infarction lesion: none, 9.7%; 1, 16.0%; >2, 34.5%; microbleeds lesion: none, 10.1%; 1, 15.3%; >2, 30.0%; periventricular hyperintensity grade: 0, 5.7%; 1, 11.5%; >2, 23.7%). The association of short OLST with lacunar infarction and microbleeds but not periventricular hyperintensity remained significant even after adjustment for possible covariates (lacunar infarction,
P
=0.009; microbleeds,
P
=0.003; periventricular hyperintensity,
P
=0.601). In contrast, no significant association was found between posturographic parameters and cSVD, whereas these parameters were linearly associated with OLST. Short OLST was also significantly associated with reduced cognitive function independent of covariates, including cSVD (
P
=0.002).

Conclusions—
Postural instability was found to be associated with early pathological changes in the brain and functional decline, even in apparently healthy subjects.
Recombinant Tissue-Type Plasminogen Activator Plus Eptifibatide Versus Recombinant Tissue-Type Plasminogen Activator Alone in Acute Ischemic Stroke
Authors: Adeoye Opeolu , Sucharew Heidi , Khoury Jane , Tomsick Thomas , Khatri Pooja , Palesch Yuko , Schmit Pamela A. , Pancioli Arthur M. , Broderick Joseph P. 
Published: December 2014

Background and Purpose—
The Combined Approach to Lysis Utilizing Eptifibatide and rt-PA in Acute Ischemic Stroke-Enhanced Regimen (CLEAR-ER) trial demonstrated safety of recombinant tissue-type plasminogen activator (r-tPA) plus eptifibatide in acute ischemic stroke (AIS). CLEAR-ER randomized AIS patients (5:1) to 0.6 mg/kg r-tPA plus eptifibatide versus standard r-tPA (0.9 mg/kg). Interventional Management of Stroke III randomized AIS patients to r-tPA plus endovascular therapy versus standard r-tPA. Albumin in Acute Stroke Part 2 randomized patients to albumin±r-tPA versus saline±r-tPA. Our aim was to compare outcomes in CLEAR-ER combination arm patients to propensity score-matched r-tPA only subjects in Albumin in Acute Stroke Part 2 and Interventional Management of Stroke III.

Methods—
The primary outcome was 90-day severity-adjusted modified Rankin score (mRS) dichotomization based on baseline National Institutes of Health Stroke Scale. Secondary outcomes were 90-day mRS dichotomization as excellent (mRS, 0–1); mRS dichotomization as favorable (mRS, 0–2); and nonparametric analysis of the ordinal mRS.

Results—

Eighty-five combination arm CLEAR-ER subjects were matched with 169 Albumin in Acute Stroke Part 2 and Interventional Management of Stroke III trials’ r-tPA only patients (controls). Median age in CLEAR-ER and control subjects was 68years; median National Institutes of Health Stroke Scale in the CLEAR-ER subjects was 11 and in control subjects 12. At 90 days, CLEAR-ER subjects had a nonsignificantly greater proportion of patients with favorable outcomes (45% versus 36%; unadjusted relative risks, 1.24; 95% confidence intervals, 0.91–1.69;
P
=0.18). Secondary outcomes were 52% versus 34% excellent outcomes (relative risks, 1.51; 95% confidence intervals, 1.13–2.02;
P
=0.007); 60% versus 53% favorable outcome (relative risks, 1.13; 95% confidence intervals, 0.90–1.41;
P
=0.31); and ordinal Cochran–Mantel–Haenszel
P
=0.10.

Conclusion—
r-tPA plus eptifibatide showed a favorable direction of effect that was consistent across multiple approaches for AIS outcome evaluation. A phase III trial to establish the efficacy of r-tPA plus eptifibatide for improving AIS outcomes is warranted.
Estimating Spatially Varying Severity Thresholds of a Forest Fire Danger Rating System Using Max-Stable Extreme-Event Modeling
Authors: Stephenson Alec G. , Shaby Benjamin A. , Reich Brian J. , Sullivan Andrew L. 
Published: December 2014

AbstractFire danger indices are used in many countries to estimate the potential fire danger and to issue warnings to local regions. The McArthur fire danger rating system is used in Australia. The McArthur forest fire danger index (FFDI) uses only meteorological elements. It combines information on wind speed, temperature, relative humidity, and recent rainfall to produce a weather index of fire potential. This index is converted into fire danger categories to serve as warnings to the local population and to estimate potential fire-suppression difficulty. FFDI values above the threshold of 75 are rated as extreme. The spatial behavior of large values of the FFDI is modeled to investigate whether a varying threshold across space may serve as a better guide for determining the onset of elevated fire danger. The authors modify and apply a statistical method that was recently developed for spatial extreme events, using a “max-stable” process to model FFDI data at approximately 17 000 data sites. The method that is described here produces a quantile map that can be employed as a spatially varying fire danger threshold. It is found that a spatially varying threshold may serve to more accurately represent high fire danger, and an adjustment is proposed that varies by local government area. Temporal change was also investigated, and evidence was found of a recent increase in extreme fire danger in southwestern Australia.
An Analysis of the Environmental Moisture Impacts of Western North Pacific Tropical Cyclones
Authors: Schenkel Benjamin A. , Hart Robert E. 
Published: December 2014

Abstract
The present study examines the environmental moisture anomalies present during western North Pacific tropical cyclone (TC) passage using storm-relative composites. Composited precipitable water anomalies reveal asymmetric anomalies with dry anomalies to the northwest and southwest of the TC and moist anomalies to the east of the TC. Precipitable water anomalies filtered in space and time suggest that the moisture anomalies in the northwest, southwest, and east regions (NWR, SWR, and ER, respectively) are partially due to the TC, while the anomalies in the SWR are also forced by a convectively suppressed Madden–Julian oscillation (MJO) and equatorial Rossby wave (ERW). Composited vertically integrated moisture budgets and backward parcel trajectories reveal that the moisture anomalies in the NWR, SWR, and ER are primarily due to the convergence of climatological mean moisture by the anomalous meridional wind. This convergence is induced by the secondary circulation of the TC in the NWR and ER and by inertial instability induced by the TC, MJO, and ERW in the SWR and ER as also suggested by prior work. Dry anomalies in the NWR are also forced by the advection of moisture by lower-tropospheric northerly wind anomalies associated with the primary circulation of the TC. Together with prior work, these results suggest that TCs can have significant impacts on their large-scale atmospheric environment extending well beyond the spatiotemporal scales of the lower-tropospheric cyclonic circulation of the TC.
Implications of Ural Blocking for East Asian Winter Climate in CMIP5 GCMs. Part I: Biases in the Historical Scenario
Authors: Cheung Hoffman H. N. , Zhou Wen 
Published: December 2014

Abstract
This study assesses the ability of the 25 GCMs from phase 5 of the Coupled Model Intercomparison Project (CMIP5) to simulate Ural blocking (UB) and its linkage with the East Asian winter climate [December–February (DJF)] in a historical run (1950/51–2004/05). A Ural blocking index (UBI) is defined as the DJF-mean blocking frequency over 45°–90°E for each winter.
Regression analyses suggest that the long-term mean bias of UBI is caused by the long-term mean circulation bias over the North Atlantic. On seasonal time scales, the GCMs simulating a positive bias of UBI are associated with a stronger Atlantic jet stream, as well as stronger westerly momentum fluxes from the North Atlantic to Europe. On synoptic time scales, however, these GCMs tend to be associated with a weaker Siberian high and East Asian trough during the evolution of a UB event. Altogether, there is no apparent linkage between the long-term mean bias of UB and the East Asian winter climate. Further studies are needed to explore the teleconnection between UB and the East Asian winter climate in the GCMs.
Preceding Factors of Summer Asian–Pacific Oscillation and the Physical Mechanism for Their Potential Influences
Authors: Liu Ge , Zhao Ping , Chen Junming , Yang Song 
Published: December 2014

Abstract
The authors explore the preceding factors of summertime Asian–Pacific Oscillation (APO) using observations and output from the NCEP Climate Forecast System version 2 (CFSv2). Results show that the winter and spring sea surface temperatures (SSTs) in the tropical central-eastern Pacific (TCEP) and the spring sea level pressure (SLP) over the north Indian Ocean (NIO) are significantly correlated with summer APO. The preceding TCEP SST anomaly tends to exert a delayed impact on summer APO through the following process. The previous winter TCEP SST anomaly persists until spring and results in SLP anomaly over the NIO in spring. The latter induces a vertical motion anomaly over the western Tibetan Plateau, which alters spring rainfall and underlying soil moisture in situ, further modulating local surface air temperature during the following summer and hence the summer APO. The CFSv2 has high skills in predicting the winter and spring TCEP SST and the spring NIO SLP and successfully captures the observed relationships of TCEP SST and NIO SLP with summer APO. This result explains why the CFSv2 is capable of predicting the summer APO teleconnection by several months in advance.
Variational Estimation of Wave-Affected Parameters in a Two-Equation Turbulence Model
Authors: Zhang Xuefeng , Han Guijun , Li Dong , Wu Xinrong , Li Wei , Chu Peter C. 
Published: December 2014

AbstractA variational method is used to estimate wave-affected parameters in a two-equation turbulence model with assimilation of temperature data into an ocean boundary layer model. Enhancement of turbulent kinetic energy dissipation due to breaking waves is considered. The Mellor–Yamada level 2.5 turbulence closure scheme (MY2.5) with the two uncertain wave-affected parameters (wave energy factor α and Charnock coefficient β) is selected as the two-equation turbulence model for this study. Two types of experiments are conducted. First, within an identical synthetic experiment framework, the upper-layer temperature “observations” in summer generated by a “truth” model are assimilated into a biased simulation model to investigate if (α, β) can be successfully estimated using the variational method. Second, real temperature profiles from Ocean Weather Station Papa are assimilated into the biased simulation model to obtain the optimal wave-affected parameters. With the optimally estimated parameters, the upper-layer temperature can be well predicted. Furthermore, the horizontal distribution of the wave-affected parameters employed in a high-order turbulence closure scheme can be estimated optimally by using the four-dimensional variational method that assimilates the upper-layer available temperature data into an ocean general circulation model.
Evaluation of bilirubin displacement effect by acetaminophen in vitro
Authors: Sugino Masahiro , Okada Hitoshi , Kusaka Takashi , Itoh Susumu 
Published: December 2014

Background The use of acetaminophen as a drug for pain control is expected to increase in the neonatal field. The displacement factor of acetaminophen in the reaction in the glucose oxidase peroxidase method is very high, but is also considered to be inaccurate based on physicochemical properties. Method Unbound bilirubin was measured using the erythrocyte-bound bilirubin measurement method and glucose oxidase peroxidase method by the addition of acetaminophen or sulfisoxazole. The displacement factor was measured using glucose oxidase peroxidase method with the addition of tert-butyl-p-hydroxyanisole. Results Acetaminophen did not increase erythrocyte-bound bilirubin, and the addition of tert-butyl-p-hydroxyanisole lowered its displacement factor. On the other hand, sulfisoxazole increased erythrocyte-bound bilirubin, while tert-butyl-p-hydroxyanisole did not change its displacement factor. Conclusion Acetaminophen is an accelerator of the reaction in the glucose oxidase peroxidase method and does not displace bilirubin from human serum albumin.
Effects of exercise training with traditional dancing on functional capacity and quality of life in patients with schizophrenia: a randomized controlled study
Authors: Kaltsatou A , Kouidi E , Fountoulakis K , Sipka C , Theochari V , Kandylis D , Deligiannis A 
Published: December 2014

Objective: To examine the effects of an eight-month exercise training programme with Greek traditional dancing on functional capacity and quality of life in patients with schizophrenia. Design: Randomized controlled trial. Setting: Sports Medicine Laboratory. Subjects: A total of 31 patients, aged 59.9 ± 14.1 years. Interventions: They were randomly assigned either to a Greek traditional dancing programme (Group A) or to a sedentary control group (Group B). Main measures: A functional capacity assessment was performed at baseline and the end of the study. Global Assessment of Functioning Scale and Positive and Negative Syndrome Scale were also used. Quality of life was examined using the Quality of Life and Satisfaction questionnaire. Results: After the eight months, Group A increased walking distance in the 6-minute walk test (328.4 ± 35.9 vs. 238.0 ± 47.6 m), sit-to-stand test (19.1 ± 1.8 vs. 25.1 ± 1.4 seconds), Berg Balance Scale score (53.1 ± 2.1 vs. 43.2 ± 6.7), lower limbs maximal isometric force (77.7 ± 25.7 vs. 51.0 ± 29.8 lb), Positive and Negative Syndrome Scale total score (77.0 ± 23.1 vs. 82.0 ± 24.4), Global Assessment of Functioning Scale total score (51.3 ± 15.5 vs. 47.7 ± 13.3) and Quality of Life total score (34.9 ± 5.2 vs. 28 ± 4.5), compared with Group B. Conclusions: Our results demonstrate that Greek traditional dances improve functional capacity and quality of life in patients with schizophrenia.