bluuebunny/crossref_metadata_2025_split huggingface dataset:
HLA ANTIGENS IN DERMATITIS HERPETIFORMIS AMONG JAPANESE
Authors: Hashimoto Koji , Miki Yoshiharu , Nishioka Kiyoshi , Nakata Seizo , Matsuyama Masamichi 
Published: April 2015

ABSTRACTHLA‐typing was performed on Japanese patients with dermatitis herpetiformis Duhring (DH). Only one of them showed granular deposition of IgA at dermal papillae whereas the rest showed linear IgA deposition. No statistically significant increase in the incidence of specific HLA antigen was noted. HLA‐B8 was absent in both patients and healthy controls.
THE ROLE OF MACROPHAGES IN INCONTINENTIA PIGMENTI HISTOLOGICA
Authors: Takematsu Hideaki , Seiji Makoto 
Published: April 2015

ABSTRACTIn an attempt to elucidate the mechanisms underlying the migration of macrophages towards the epidermis from the dermis in pigmentary incontinence, the chemotactic activities of a horny cell membrane fraction and melanosomes were measured using a modified Boyden chamber.Horny cell membrane fraction and melanosomes showed about twice as high a chemotactic activity for macrophages than did casein. These in vitro observations suggest that, in pigmentary incontinence, migration of macrophages into the epidermis, where they phagocytize degenerated keratinocytes and melanosomes, may be one of the consecutive steps.
AN ADULT CASE OF REACTIVE PERFORATING COLLAGENOSIS
Authors: Mohri Shinobu , Sano Tsutomu , Fukuda Shigeru 
Published: April 2015

ABSTRACTA case of reactive perforating collagenosis (RPC) is reported. The patient was a 20‐year‐old man with suspected Wegener's granulomatosis, who had asymptomatic papular lesions on his elbows. Histologically these papules were compatible with RPC. Electron microscopic studies showed masses of fine filamentous material around well‐developed histiocytes. The differentiation from collagenoma perforans verruciforme (CPV) is discussed.
DISSEMINATED ZOSTER
Authors: Nigam Pranesh , Dayal S.G. , Dubey A.L. 
Published: April 2015

ABSTRACTChicken pox and herpes zoster are different manifestations of infection by the same viral agent, but the simultaneous occurrence of both in the same patient is uncommon. In this communication we present seven cases of concurrent herpes zoster and chicken pox. Adults were frequently involved, with a male to female ratio of 2.5:1. Varicelliform eruptions followed unilateral herpetic attacks within a 5–8 day interval. Milliary tuberculosis was associated in two cases and cervical tuberculous lymphadenitis in one case. Fever and pain (localised to affected areas) appeared 5–7 days before (5 cases) or concomittant with the appearance of skin eruptions (2 cases). The skin lesions started with local redness followed by red papules which progressed to vesicular, pustular and crusting stages over the next two weeks. The lesions were arranged unilaterally in characteristic bandlike clusters, following radicular lines. The trigeminal nerve was involved in one case. Another patient developed Ramsay Hunt Syndrome (Facial nerve palsy) after seven days of herpetic lesions. Post‐herpetic neuralgia present in two cases was treated effectively with corticosteroids and residual post‐herpetic hyperasthesia improved gradually. Carbamezapine (Mazetol) 100–300 mg/day helped in relieving pain. Corticosteroids proved to be beneficial in reducing severity and morbidity of the disease.
ADENOSINE DEAMINASE ACTIVITY IN PERIPHERAL LYMPHOCYTES OF PSORIASIS AND SÉZARY'S SYNDROME
Authors: Iizuka Hajime , Koizumi Hiroko , Kimura Tetsunori , Miura Yusho 
Published: April 2015

ABSTRACTAdenosine deaminase (ADA) activity in peripheral lymphocytes were measured in 15 normal subjects, 15 psoriatic patients, and one patient with Sézary's syndrome.No significant differences were found in ADA activity between normal and psoriatic lymphocytes. A significant increase in ADA activity was observed in lymphocytes from the patient with Sézary's syndrome. The increased ADA activity in Sézary's syndrome was not accompanied by a change in the Km value for adenosine.The possible significance of the elevation of ADA activity in T‐lymphocyte dysfunction was discussed.
THE ROLE OF MITE, HOUSE DUST AND CANDIDA ALLERGENS IN CHRONIC URTICARIA
Authors: Numata Tsunemi , Yamamoto Shoso , Yamura Takuso 
Published: April 2015

ABSTRACTIntradermal skin tests and in vitro antigen‐induced histamine release from leucocytes were carried out using mite, house dust and candida allergens in 49 patients with chronic urticaria in whom the cause was not identifiable from the history. None of them had other atopic diseases. Among the 49 cases, the number of patients who gave positive skin reactions to different allergens was as follows: mite, 24; house dust, 18; candida, 17. Significant histamine release from leucocytes by mite antigen was observed in 8 out of 12 patients who had 2+ or 3+ skin reactions. The present results suggest that mite, house dust and candida allergens may play a role in chronic urticaria, and especially that the mite may be one of the possible allergens in chronic urticaria.
PROLIDASE ACTIVITY DURING HEALING OF SKIN BURNS IN RATS
Authors: Umemura Shigeo , Arata Jirō , Nohara Nozomi 
Published: April 2015

ABSTRACTThe activity of prolidase (Imidodipeptidase, EC 3.4.3.7.) in burned rat skin was assayed. A few days after burning, the prolidase activity in burned skin was higher than that in controls. The results showed that prolidase activity increased during wound healing.
GENERALIZED HERPES SIMPLEX
Authors: Otsuka Fujio , Niimura Michihito , Harada Shotaro , Ishibashi Yasumasa , Shishiba Takako 
Published: April 2015

ABSTRACTA 41‐year‐old Japanese man with a 11‐year history of Hailey‐Hailey's disease on his groin developed generalized herpes simplex on his groin, throat, lips and extremities which, to our knowledge, has not been previously described.Histological examinations using light and electron microscopes showed herpes virus infection of the skin. Virus culture and serological methods revealed this case to be a primary infection of type 1 herpes simplex virus.
FIBROBLASTS AND IMMUNE COMPLEXES
Authors: Nakaye Masako , Ueki Hiroaki 
Published: April 2015

ABSTRACTCultured human fibroblasts were incubated with in vitro‐prepared insoluble immune complexes consisting of horseradish‐peroxidase (HRP)‐anti HRP for 4, 24 or 48 hours. These incubated fibroblasts were then processed for electron microscopy. Peroxidase‐positive immune complexes were observed both on the surfaces and within the cytoplasms of the fibroblasts. However, the frequency of interiorized immune complexes or phagosomes was not high. It was speculated that, in connective tissue, fibroblasts can also adhere to and trap immune complexes in vivo, thus eliminating them from tissues under certain conditions.
FIBROBLASTS AND IMMUNE COMPLEXES
Authors: Ueki Hiroaki , Nakaye Masako 
Published: April 2015

ABSTRACTCultured human fibroblasts of 20 passages were incubated with in vitro‐prepared complement‐fixed insoluble immune complexes for 4 or 24 hours. Three lysosomal enzymes, β‐glucuronidase, N‐acetyl‐β‐glucosaminidase and acid phosphatase, were assayed in intra‐cellular and extra‐cellular samples. The three enzymes' activities were shown to increase remarkably in the intra‐cellular samples, but little in the extra‐cellular samples. These enzymes appeared to increase in or be released from the fibroblasts incubated with the immune complexes. Fibroblasts may play some role in eliminating immune complexes from the skin and in inducing inflammatory reactions.
RETINOID INHIBITS KERATIN FORMATION OF PIG SKIN EXPLANTS
Authors: Aoyagi Takashi , Kamigaki Katsuko , Saruta Motoji , Iizuka Hajime , Miura Yusho 
Published: April 2015

ABSTRACTUsing a pig explant culture system, the effects of retinoids on keratin formation in pig epidermal cells were studied. The SDS‐PAGE patterns of keratin extracted from the outgrowing epidermis in vitro were different from those of pig epidermis in vivo. The whole pig epidermis has four major keratin bands (M.W. 65,000, 60,000, 56,000 and 51,000), while the outgrowing epidermis has three major keratin bands (M.W. 63,000, 59,000 and 52,000). The SDS‐PAGE patterns of keratin proteins extracted from the outgrowing epidermis treated with Ro 10‐1670 were markedly altered. The highest molecular protein (M.W. 63,000) found in control explant was almost entirely absent in Ro 10‐1670‐treated explants and the second band (M.W. 59,000) found in control explants was split into two minor bands (M.W. 59,000 and 58,000).These results indicated that Ro 10‐1670 strongly modified keratin formation in vitro explant culture of pig skin.
ULTRASTRUCTURE OF SO‐CALLED ANGIOBLASTOMA OF THE SKIN BEFORE AND AFTER SOFT X‐RAY THERAPY
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTTissues from a two‐month‐old Japanese male infant with a typical angioblastoma of the skin on the right groin was examined by light and electron microscopy before and after soft X‐ray therapy. Before therapy, nest‐like proliferation of the tumor cells with frequent formation of vascular spaces was observed. Ultrastructurally, these tumor cells showed the morphological characteristics of vascular endothelial cells in their size, shape and usual organelles. These cells usually had an abundance of peculiar dense granules with a limiting membrane. Fibrin deposits and thrombosis with aggregations of platelets were observed in the lumina in situ. After soft X‐ray therapy with a total dose of 3,600 rads, disappearance of most of the tumor cell‐nests and prominent dilatation of the residual vessels were observed. Ultrastructurally, numbers of the vessels showed more or less degenerative and destructive changes. Vessels with ruptured endothelial cell linings, extravasated red cells, and hemosiderin deposits were also frequently observed. From these findings, it is concluded that angioblastoma of the skin should be considered as one of the benign hemangiomas of the skin, and that degenerative changes in the endothelial cells induced by irradiation are almost identical to those in other conditions.
PATHOGENESIS OF GENETIC HUMAN HAIR DISORDERS
Authors: Aoyagi Takashi , Miura Yusho , Porter Paul S. 
Published: April 2015

ABSTRACTSeveral distinct morphological abnormalities of hair were discussed. Most of congenital hair defects appear to be related to anatomic (defective cuticle formation) and biochemical (reduced sulfur content) properties.The major structural hair abnormalities include defects in the cuticle, cortex and medulla. Our studies have revealed that there were defects of the cuticle in each case of a major structural hair defect.
ATYPICAL TYROSINASE POSITIVE ALBINOS
Authors: Mizoguchi Masako , Ishibashi Yasumasa , Hsin‐Su Yu , Iijima Masafumi , Hori Yoshiaki , Kukita Atsushi 
Published: April 2015

ABSTRACTUsing light and electron microscopy, we studied five cases of tyrosinase positive albinism, with special reference to melanization of the hair‐bulbs before and after incubation with L‐tyrosine. Electron microscopic autoradiographs of the hair‐bulbs after incubation with 3H‐L‐tyrosine were also investigated. As a result, it was found that three cases could be categorized as ordinary tyrosinase positive albinos, but the other two could not. It was concluded that tyrosinase positive albinism might not be a homogeneous disease, but a disease which can be divided into several subclasses.
GLYCOSAMINOGLYCAN IN THE SKIN AND URINE OF A HUNTER SYNDROME PATIENT WITH A SPECIFIC SKIN LESION
Authors: Nakamura Toshitaka , Nishida Kenju , Matsuda Kazuko , Kitano Yukio , Sano Shigeharu , Yutaka Tohru 
Published: April 2015

ABSTRACTThe glycosaminoglycan (GAG) contents of the cutaneous papules, the non‐eruptive skin, and the urine of a patient with Hunter syndrome were examined. Both skin samples contained hyaluronic acid (HA) and dermatan sulfate (DS) as major components and heparan sulfate (HS) and chondroitin sulfate (CS) as minor components. The HA content of the papules was greatly increased, while that of the non‐eruptive skin was normal. The amounts of DS and HS were increased in all three samples; the papules, non‐eruptive skin, and urine. HS from the patient's skin and urine had an electrophoretic mobility different from that of authentic HS. It seemed interesting from a pathogenetic viewpoint that the components of GAG in the cutaneous papules differed from those in the non‐eruptive skin and urine.
AN ULTRASTRUCTURAL STUDY OF STEATOCYSTOMA MULTIPLEX AND THE NORMAL PILOSEBACEOUS APPARATUS
Authors: Kimura Shunji 
Published: April 2015

ABSTRACTIn order to clarify the site(s) from which steatocystoma multiplex (SCM) originates, five cases of SCM and the normal pilosebaceous apparatus were comparatively examined by electron microscopy. The cyst‐wall of SCM, consisting either of flattened or of undulating horny layer, contained a small number of tonofilaments, desmosomes, and tiny spherical keratohyalin granules. There were variable numbers of lamellar granules which were also frequently observed in the intercellular spaces of the horny layer. Three of the SCM cases included sebaceous cells. The contents of the cysts were mostly amorphous and short‐fibrillar, but sometimes keratinized cells and remnants of lamellar granules were observed. These findings were analogous to those of the normal pilosebaceous apparatus from the level of the lower part of the infundibulum to the outer root sheath Via the sebaceous duct, and were specifically more analogous to those of the sebaceous duct.
DETERMINATION OF HYALURONIC ACID IN THE URINE OF A PATIENT WITH WERNER'S SYNDROME
Authors: Maekawa Yoshihiro , Hayashibara Toshio 
Published: April 2015

ABSTRACTA 54‐year‐old Japanese male with Werner's syndrome was reported. We found increased excretion of hyaluronic acid in the urine, although the content and analyses of the glycosaminoglycans in the urine eluted by Dowex‐1 column chromatography was shown to be hyaluronic acid by comparison with standard hyaluronic acid. The Sephadex G‐200 gel filtration of the 0.5 M NaCl fraction caused separation into two fractions. It was revealed that there was not only an excessive production of hyaluronic acid, but also an excretion of lower molecular weight hyaluronic acid in the urine of this syndrome.
THREE CASES OF PRURIGO PIGMENTOSA
Authors: Yamasaki Reiko , Dekio Satoshi , Moriyasu Shojiro , Takagaki Kenji 
Published: April 2015

ABSTRACTThree cases of prurigo pigmentosa are presented. These patients had episodes of recurring pruritic red papules and gross reticular pigmentation, and responded remarkably to 4,4'‐diaminodiphenyl sulfone (DDS) or sulfisomezole. The possibility was suggested that this dermatosis is due to allergic reactions to substances similar in chemical structure to sulfonamides.
CONGENITAL ONYCHODYSPLASIA OF THE INDEX FINGERS
Authors: Kikuchi Ichiro , Ishii Yoshimitsu , Idemori Masahiro , Ogata Katsumi 
Published: April 2015

ABSTRACTTwo cases of congenital onychodysplasia of the index fingers (COIF), one of them with an abnormal range of active motion of the digits, were reported. The characteristic location of a nail, or nails in micronychia and polyonychia of COIF and the involvement of the nail and the bone may be explained by the anatomy of the digit. That is, the radially more pronounced involvement of the nail may be related to the relatively smaller size of the artery of the radial aspect. This may support the transient ischemia theory of Ohta and Haseda (1) in fetal life, though the true etiology of COIF still remains obscure.
THE INDUCTION AND ELICITATION OF DISEASE IN HUMAN SKIN
Authors: Shelley Walter B. 
Published: April 2015

ABSTRACTThe skin provides a unique laboratory in which to study disease under strictly controlled experimental conditions. In this laboratory, a wide variety of diseases have been experimentally replicated over the past two hundred years. Such experimental models have added greatly to our understanding of the pathogenesis and the nature of disease in general as well as the disease under study.Specific review is made of our own experience in the field of experimental skin disease in man. This includes the controlled reproduction of miliarias, hidradenitis suppurativa, pruitus, HalowaxR acne, immune granulomas, and erythema multiforme. From these studies come a powerful awareness of individual differences in susceptibility, the hazards of initiating certain diseases for which there is no specific treatment and a new understanding of sarcoidosis and erythema multiforme.
AN IN VIVO AND IN VITRO STUDY OF THE CYCLOPHOSPHAMIDE‐INDUCED ENHANCEMENT OF CONTACT SENSITIVITY TO 2,4‐DINITRO‐1‐CHLOROBENZENE (DNCB)
Authors: Ikezawa Zenro 
Published: April 2015

ABSTRACT
Enhancement of DNCB contact sensitivity (CS) reactions by cyclophosphamide (Cy) was observed in guinea pigs sensitized by painting or footpad injection of Freund's incomplete adjuvant, in which immunologic suppression of CS had been induced, but not in animals sensitized by footpad injection of Freund's complete adjuvant. The latter developed markedly strong CS reactions.
The effects of Cy on in vivo skin reactions and on in vitro endogenous and antigen‐induced DNA synthesis by the draining lymph node cells in animals sensitized topically with DNCB were studied on various days after sensitization. Cy treatment 3 days before sensitization augmented CS reactions. This augmentative effect was found from day 5 to day 21. Cy treatment enhanced in vitro antigen‐induced DNA synthesis as well as endogenous DNA synthesis by the draining lymph node cells. Cy‐sensitive cells and their progeny may be involved in suppression of the in vitro specific stimulation of the primed cells as well as endogenous cell proliferation for priming.
The effect of DNCB‐sensitization on the in vitro concanavalin A (ConA) dose‐response of the spleen cells and lymph node cells was studied. By day 7 after sensitization, the stimulation of the spleen cells by low doses of ConA had decreased, but the responses to high doses of mitogen had increased. This bidirectional effect of DNCB‐sensitization on ConA responses was discussed.
SYRINGOMA LIMITED TO GENITALIA OF A PREADOLESCENT GIRL
Authors: Dekio Satoshi , Maehama Yoshiko 
Published: April 2015

ABSTRACTSyringoma limited to the female genitalia is presented. Many small papules were seen on only the labia majora of a 9‐year‐old Japanese girl. Skin biopsy confirmed the diagnosis of syringoma.
URINARY PORPHYRIN ANALYSES IN PATIENTS WITH PORPHYRIA CUTANEA TARDA
Authors: Nonaka Shigeo , Ogami Taro , Yoshida Kazunori , Honda Tetsuzo , Murayama Fumio , Yoshida Hikotaro 
Published: April 2015

ABSTRACTUrinary porphyrin analyses were carried out in five patients with porphyria cutanea tarda and in a control group of 44 individuals. Quantitative analysis revealed that the mean value of coproporphyrin was 67.3 μg/l (range 13.1–189.1 μg/l) in 44 normal individuals and the mean value of uroporphyrin was 9.7 μg/l (range 5.0 to 13.7 μg/l) in 11 of 44 controls, but not detectable in the remainder. Four of the five patients with porphyria cutanea tarda, however, had markedly elevated coproporphyrin and uroporphyrin levels. Urinary porphyrin pattern analysis was carried out using one dimentional thin layer chromatography. The pattern in controls revealed that the mean value of coproporphyrin was 85.5%, while the other fractions consituted less than 6.0%. In the porphyria cutanea tarda group, however, hepta‐carboxyl porphyrin and uroporphyrin were predominant in two; all five fractions appeared in roughly equal amounts in one; and in the remaining two, coproporphyrin was decreased to a value of only about 50%.A patient treated by phlebotomy was also evaluated by this method. His urinary porphyrin pattern showed a shift from uroporphyrin to a high concentration of coproporphyrin during and after the treatment.With this method, it is very simple to obtain data for urinary porphyrin pattern analysis, and many samples can be analyzed simultaneously. The profile can aid in the diagnosis of porphyria cutanea tarda, especially when the patients show low levels of urinary porphyrins quantitatively.
A CASE OF MOSQUITO HYPERSENSITIVITY TERMINATING AS MALIGNANT HISTIOCYTOSIS
Authors: Mohri Shinobu , Kawashima Yoshio , Uchigata Yasuko , Seki Hidetoshi , Okuda Norihiko , Masuda Shinji , Fukushiro Ryoichi 
Published: April 2015

ABSTRACTA patient with a 5 year history of hypersensitivity to mosquitoes developed a skin nodule on the chest, high fever, and hepatosplenomegaly. The diagnosis of malignant histiocytosis was made, based upon histological examination of the skin and cytological study of the pleural effusion. Immunological studies revealed the impaired activity of both T and B cells. After four weeks of no response to chemotherapy, the patient died. Autopsy revealed malignant histiocytes invading the bone marrow, liver, spleen, lung, adrenal glands, lymph nodes and skin. The possible relationship of immunodeficiency to malignant histiocytosis is discussed.
SUBUNGUAL MYXOID CYST PRODUCING AN ULCER IN THE NAIL PLATE
Authors: Tomoda Tetsuro , Ono Tomomichi , Ohyama Katsuro , Kojo Yasuko 
Published: April 2015

ABSTRACTA case of cutaneous myxoid cyst with an ulcer on the nail plate of the right great toe is presented. This should be differentiated from subungual malignant melanoma.
BIOCHEMICAL ANALYSES OF STRATUM CORNEUM IN HYPERKERATOSIS LENTICULARIS PERSTANS
Authors: Negi Makoto , Kubo Yukari , Yoshiike Takashi , Manabe Motomu , Hirotani Tetsuya , Ogawa Hideoki 
Published: April 2015

ABSTRACTThe stratum corneum obtained from keratotic papules of hyperkeratosis lenticularis perstans was fractionated into keratin filamentous, water soluble, and membraneous fractions. SDS‐gel electrophoresis was used to examine the keratin filamentous and soluble fractions, and amino acid analysis was employed for all fractions.Electrophoresis of the keratin filamentous fraction revealed no remarkable changes in the pattern of gel bands, compared with normal controls. On the other hand, the pattern observed in the water soluble fraction showed a decrease in the number and intensity of staining bands with molecular weights within the range of 8,000–10,000.In the amino acid analysis, the half‐cystine contents of the patients' membraneous fractions were remarkably lower than those of normal membraneous fractions. Additionally, ornithine and citrulline, which are not usually found as components of protein, were determined to be moderately high in all fractions.
URTICARIAL VASCULITIS WITH BLISTER FORMATION: BULLOUS (VESICULAR) URTICARIAL VASCULITIS
Authors: Naito Yoshiko , Nishioka Kiyoshi , Sarashi Chizuko , Sano Shigeharu , Yonemasu Kunio 
Published: April 2015

ABSTRACTA 37‐year‐old woman developed blistering on urticarial erythematous lesions. She had multiple joint pain, subfever, alopecia, high antinuclear factor, and hypocomplementemia. She was diagnosed as having SLE. Her SLE symptoms responded well to steroid therapy, whereas her urticarial lesions responded to DDS but not to steroid. Histology of her skin lesions resembled that of dermatitis herpetiformis. We suggested the term “bullous (Vesicular) urticarial vasculitis” to distinguish such a case with peculiar manifestations in urticarial vasculitis.
ULTRASTRUCTURAL OBSERVATIONS OF FUNGAL ELEMENTS IN A TRICHOSPORON CUTANEUM INFECTION OF THE SKIN
Authors: Otsuka Fujio , Seki Yoshihito , Takizawa Kiyohiro , Kukita Atsushi 
Published: April 2015

ABSTRACTUsing electron microscopic methods, we observed the fine structure of fungal elements occurring in a facial granulomatous infection caused by Trichosporon cutaneum.Two types of fungal elements were recognized: (1) fungal elements attached to phagocytes or just ingested into phagocytes and (2) fungal elements located within the phagocytes in the process of being digested.All the fungal elements observed had been denatured to varying degrees. In these denatured fungal elements, the intracellular organelles had disappeared. Fibrillar structures derived from the cell wall were recognized.