bluuebunny/crossref_metadata_2025_split huggingface dataset:
ATM, THMS, and RRM1 protein expression in nasopharyngeal carcinomas treated with curative intent
Authors: Ko Jenny Jaeeun , Klimowicz Alexander C. , Jagdis Amanda , Phan Tien , Laskin Janessa , Lau Harold Y. , Siever Jodi E. , Petrillo Stephanie K. , Thomson Thomas A. , Rose M. Sarah , Bebb Gwyn , Magliocco Anthony M. , Hao Desirée 
Published: January 2015

AbstractBackgroundIn advanced nasopharyngeal carcinoma (NPC), biomarkers may help predict survival.MethodsTumoral expression of ataxia‐telangiectasia mutated (ATM), thymidylate synthetase (THMS), and ribonucleotide reductase subunit M1 (RRM1), was correlated with survival in patients with nonmetastatic NPC using quantitative fluorescence immunohistochemistry with automated quantitative digital image analysis.ResultsOf the 146 patients included, 58 patients (40%) received concurrent chemoradiation therapy; the remainder was treated with radiation. Overall survival (OS) at 5 years was 71% (95% confidence interval [CI], 62% to 78%); disease‐free survival (DFS) was 48% (95% CI, 39% to 57%). OS worsened for increasing values of ATM (hazard ratio [HR], 2.83; 95% CI, 1.01–7.94; p = .049) for values greater than the 75th percentile compared to less than the 25th percentile, but improved for tumors with higher THMS levels (HR, 0.44; 95% CI, 0.20–0.94; p = .033) for values greater than the 25th percentile compared to less than or equal to the 25th percentile. RRM1 was not associated with OS (p = .748). No biomarkers were associated with DFS.ConclusionIn our cohort, relative overexpression of ATM and low THMS levels were associated with worse OS. © 2015 Wiley Periodicals, Inc. Head Neck 38: E384–E391, 2016
Intratumoral lymphatic vessel density as a predictor of progression‐free and overall survival in locally advanced laryngeal/hypopharyngeal cancer
Authors: Hurst Newton J. , Dominello Michael , Dyson Gregory , Jaratli Hayan , Sharma Meenu , Ahmed Yasin K. , Melkane Antoine E. , Rose Christopher , Jacobs John , Giorgadze Tamar , Kim Harold 
Published: January 2015

AbstractBackgroundLymphatic vessel density (LVD) has been shown to be an important predictor of survival in head and neck cancers. We report the predictive value of LVD for progression‐free survival (PFS) and overall survival (OS) in laryngeal/hypopharyngeal cancer.MethodsFifty‐five untreated patients with T3/T4 laryngeal and T4 hypopharyngeal cancer underwent laryngectomy between 1999 and 2010. Surgical specimens were immunostained with D2‐40, a specific lymphatic marker. LVDs were determined in tumor vessel “hot spots.” Recursive partitioning analysis identified LVD thresholds for both peritumoral (LVDpt) and intratumoral (LVDit) vessels for association with PFS and OS.ResultsPatients with mean LVDit of <11 vessels/mm2 had 2‐year PFS and OS rates of 58% and 65%, respectively, compared to 13% and 13% for those with LVDit ≥11 vessels/mm2 (p = .06 and .04, respectively).ConclusionIntratumoral lymphatic vessel density is predictive of PFS and OS in locally advanced laryngeal/hypopharyngeal cancer. © 2015 Wiley Periodicals, Inc. Head Neck 38: E417–E420, 2016
The effect of strip thinning on spatial and temporal variability of throughfall in a Japanese cypress plantation
Authors: Sun Xinchao , Onda Yuichi , Chiara Stefani , Kato Hiroaki , Gomi Takashi 
Published: January 2015

AbstractWe examined the effect of strip thinning on the spatio‐temporal variability of throughfall (TF) in a mature Japanese cypress (Chamaecyparis obtusa) stand. Strip thinning, which removed 50% of stems, was performed in a catchment in October 2011. TF was examined by setting up 20 tipping‐bucket rain gauges in an experimental plot. Canopy interception parameters were derived from high‐temporal‐resolution TF data. Observations were obtained during the growing season in the pre‐thinning and post‐thinning periods. The results demonstrated that after thinning, the TF rate increased from 64.3 to 76.2%, and the mean coefficient of variation of the TF rate significantly decreased from 27.3 ± 9.7 to 22.5 ± 6.8%. The spatial TF patterns were largely related to the rainfall class. There was no significant difference in the TF rate for the rainfall amount >25 mm between the periods. The temporal persistence of the normalized TF was slightly stable in the post‐thinning period and was not significantly related to canopy cover or the distance to the nearest trunk of each rain gauge in either period. Additionally, thinning resulted in an increase in the proportion of direct TF (from 0.14 ± 0.07 to 0.33 ± 0.11) and a decrease in canopy water storage (from 2.4 ± 0.7 to 1.5 ± 0.5 mm). The quantification of the canopy interception parameters can aid in modelling the changes in interception loss and its processes induced by thinning. These findings also help recognize the changes in spatial TF patterns under different forest management practices. Copyright © 2015 John Wiley & Sons, Ltd.
Modelling Technical Efficiency in Cross Sectionally Dependent Stochastic Frontier Panels
Authors: Mastromarco Camilla , Serlenga Laura , Shin Yongcheol 
Published: January 2015

SummaryThis paper proposes a unified framework for accommodating both time and cross‐sectional dependence in modelling technical efficiency in stochastic frontier models by combining the exogenously driven factor‐based approach and an endogenous threshold efficiency regime selection mechanism. This approach is able to deal with both weak and strong cross‐sectional dependence in a flexible manner. Using a dataset of 26 OECD countries over 1970–2010, we provide satisfactory estimation results for the production technology parameters and the associated efficiency ranking of individual countries. We find a positive spillover effect on efficiency, supporting the hypothesis that knowledge spillover is more likely to be induced by technological proximity. Furthermore, our approach enables us to identify efficiency clubs endogenously. Copyright © 2015 John Wiley & Sons, Ltd.
Quantitative toxicoproteomic analysis of zebrafish embryos exposed to a retinoid X receptor antagonist UVI3003
Authors: Zheng Liang , Yu Jianlan , Shi Huahong , Xia Liang , Xin Qi , Zhang Qiang , Zhao Heng , Luo Ji , Jin Wenhai , Li Daoji , Zhou Junliang 
Published: January 2015

AbstractRetinoid X receptor (RXR) antagonists, including some environmental endocrine disruptors, have a teratogenic effect on vertebrate embryos. To investigate the toxicological mechanism on the protein expression level, a quantitative proteomic study was conducted to analyze the proteome alterations of zebrafish (Danio rerio) embryos exposed to gradient concentrations of a representative RXR antagonist UVI3003. Using isobaric Tags for Relative and Absolute Quantitation (iTRAQ) labeling coupled nano high‐performance liquid chromatography‐tandem mass spectrometry (nano HPLC‐MS/MS), in total 6592 proteins were identified, among which 195 proteins were found to be differentially expressed by more than a two‐fold change in exposed groups compared with the control. Gene ontology analysis showed that these differential proteins were mostly involved in anatomical structure development, biosynthetic process, ion binding and oxidoreductase activity. Moreover, the biological pathways of translation, lipoprotein metabolism, cell survival and gluconeogenesis were intensively inhibited after exposure. Some significantly downregulated proteins such as apolipoprotein A‐I and vitellogenin and upregulated proteins such as calcium activated nucleotidase 1b, glutathione S‐transferase and glucose 6‐dehydrogenases showed a strong dose‐dependent response. The results provided new insight into the molecular details of RXR antagonist‐induced teratogenicity and added novel information of pathways and potential biomarkers for evaluation of RXR interfering activity. Copyright © 2015 John Wiley & Sons, Ltd.
Disruption of Scube2 Impairs Endochondral Bone Formation
Authors: Lin Yuh-Charn , Roffler Steve R. , Yan Yu-Ting , Yang Ruey-Bing 
Published: January 2015

ABSTRACT

Signal peptide-CUB-EGF domain-containing protein 2 (SCUBE2) belongs to a secreted and membrane-tethered multidomain SCUBE protein family composed of three members found in vertebrates and mammals. Recent reports suggested that zebrafish scube2 could facilitate sonic hedgehog (Shh) signaling for proper development of slow muscle. However, whether SCUBE2 can regulate the signaling activity of two other hedgehog ligands (Ihh and Dhh), and the developmental relevance of the SCUBE2-induced hedgehog signaling in mammals remain poorly understood. In this study, we first showed that as compared with SCUBE1 or SCUBE3, SCUBE2 is the most potent modulator of IHH signaling in vitro. In addition, gain and loss-of-function studies demonstrated that SCUBE2 exerted an osteogenic function by enhancing Ihh-stimulated osteoblast differentiation in the mouse mesenchymal progenitor cells. Consistent with these in vitro studies and the prominent roles of Ihh in coordinating skeletogenesis, genetic ablation of Scube2 (–/–) caused defective endochondral bone formation and impaired Ihh-mediated chondrocyte differentiation and proliferation as well as osteoblast differentiation of –/– bone-marrow mesenchymal stromal-cell cultures. Our data demonstrate that Scube2 plays a key regulatory role in Ihh-dependent endochondral bone formation. © 2015 American Society for Bone and Mineral Research.
Adverse Effects of Osteocytic Constitutive Activation of ß-Catenin on Bone Strength and Bone Growth
Authors: Chen Sixu , Feng Jianquan , Bao Quanwei , Li Ang , Zhang Bo , Shen Yue , Zhao Yufeng , Guo Qingshan , Jing Junjun , Lin Shuxian , Zong Zhaowen 
Published: January 2015

Abstract

The activation of the canonical Wnt/β-catenin signaling pathway in both mesenchymal stem cells and osteoblasts has been demonstrated to increase bone mass, showing promise for the treatment of low bone volume conditions such as osteoporosis. However, the possible side effects of manipulating this pathway have not been fully addressed. Previously, we reported that the constitutive activation of ß-catenin in osteoblasts impaired vertebral linear growth. In the present study, β-catenin was constitutively activated in osteocytes by crossing Catnb+/lox(exon 3) mice with dentin matrix protein 1(DMP1)-Cre transgenic mice, and the effects of this activation on bone mass, bone growth and bone strength were then observed. DMP1-Cre was found to be predominantly expressed in osteocytes, with weak expression in a small portion of osteoblasts and growth plate chondrocytes. After the activation, the cancellous bone mass was dramatically increased, almost filling the entire bone marrow cavity in long bones. However, bone strength decreased significantly. Thinner and more porous cortical bone along with impaired mineralization were responsible for the decrease in bone strength. Furthermore, the mice showed shorter stature with impaired linear growth of the long bones. Moreover, the concentration of serum phosphate decreased significantly after the activation of ß-catenin, and a high inorganic phosphate (Pi) diet could partially rescue the phenotype of decreased mineralization level and impaired linear growth. Taken together, the constitutive activation of β-catenin in osteocytes may increase cancellous bone mass; however, the activation also had adverse effects on bone strength and bone growth. These adverse effects should be addressed before the adoption of any therapeutic clinical application involving adjustment of the Wnt/β-catenin signaling pathway. © 2015 American Society for Bone and Mineral Research.
Direct fermentation of potato starch and potato residues to lactic acid by Geobacillus stearothermophilus under non‐sterile conditions
Authors: Smerilli Marina , Neureiter Markus , Wurz Stefan , Haas Cornelia , Frühauf Sabine , Fuchs Werner 
Published: January 2015

AbstractBACKGROUNDLactic acid is an important biorefinery platform chemical. The use of thermophilic amylolytic microorganisms to produce lactic acid by fermentation constitutes an efficient strategy to reduce operating costs, including raw materials and sterilization costs.RESULTSA process for the thermophilic production of lactic acid by Geobacillus stearothermophilus directly from potato starch was characterized and optimized. Geobacillus stearothermophilus DSM 494 was selected out of 12 strains screened for amylolytic activity and the ability to form lactic acid as the major product of the anaerobic metabolism. In total more than 30 batches at 3–l scale were run at 60 °C under non‐sterile conditions. The process developed produced 37 g L−1 optically pure (98%) L‐lactic acid in 20 h from 50 g L−1 raw potato starch. As co‐metabolites smaller amounts (<7% w/v) of acetate, formate and ethanol were formed. Yields of lactic acid increased from 66% to 81% when potato residues from food processing were used as a starchy substrate in place of raw potato starch.CONCLUSIONSPotato starch and residues were successfully converted to lactic acid by G. stearothermophilus. The process described in this study provides major benefits in industrial applications and for the valorization of starch‐rich waste streams. © 2015 The Authors.Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Phosphate removal from aqueous solution using a hybrid impregnated polymeric sorbent containing hydrated ferric oxide (HFO)
Authors: You Xialei , Guaya Diana , Farran Adriana , Valderrama César , Cortina José Luis 
Published: January 2015

AbstractBACKGROUNDThis study evaluated a polymeric ion exchanger impregnated with nanoparticles of hydrated ferric oxide as a selective sorbent for anionic P(V) species. The hybrid impregnated anion exchanger (HAIX) employed as sorbent combines the durability and mechanical strength of a polymeric weak base anion exchange resin with the high sorption affinity of hydrated ferric oxide towards P(V) species.RESULTSThe P(V) loading capacity of the sorbent at a pH simulating the expected conditions in treated wastewaters was 92 ± 3 mg P‐PO4 g−1 in single component solutions. The sorption of P(V) is affected slightly by the common ions typically present in these effluents such as Cl−, SO42− and NO3−, and a reduction of only 5% was observed (89 ± 2 mg P‐PO4 g−1) in multicomponent solutions. The sorbent can be regenerated (95 ± 3%) using 4% (w/w) sodium hydroxide solution.CONCLUSIONSHAIX displays high selectivity for phosphate and small affinity for SO42−, NO3− and Cl− ions, which provides excellent conditions for its application at pH values typical of secondary wastewater effluents. HAIX exhibits higher and competitive phosphate removal capacity compared with data reported in the literature for materials based on HFO and for adsorbents prepared by impregnation of HFO onto polymeric supports. © 2015 Society of Chemical Industry
Influence of polymeric materials on the performance of a mesophilic biotrickling filter treating an α‐pinene contaminated gas stream
Authors: Montes María , Veiga María C , Kennes Christian 
Published: January 2015

AbstractBACKGROUNDRecent studies have characterized the most effective absorbent polymers for the removal of volatile hydrophobic pollutants from air. This study evaluates the performance of a laboratory scale α‐pinene‐degrading biotrickling filter (BTF) packed with lava rock. A second solid phase, Hytrel® G3548L (5%, v/v), was added in order to check its effect on the performance of the system during long‐term operation.RESULTSThe biodegradation profile was similar in both bioreactor configurations, with or without solid polymers, reaching a maximum elimination capacity (ECmax) around 25.6 g m−3 h−1 when an inlet loading rate of 57.2 g m−3 h−1 was applied. Tracer studies were carried out in order to determine the effect of the liquid and gas velocities, and to analyze the gas distribution with the different packing materials.CONCLUSIONSDeviation from ideal flow caused by channelling of the fluid was observed from tracer studies. No better performance was observed under steady‐state conditions in the presence of polymeric Hytrel material. Further considerations are needed for the selection of an appropriate absorbent polymer for α‐pinene removal. © 2015 Society of Chemical Industry
Adsorption kinetics of DowexTM OptiporeTM L493 for the removal of the furan 5‐hydroxymethylfurfural from sugar
Authors: IJzer Anne Corine , Vriezekolk Erik , Ðekic Zivkovic Tanja , Nijmeijer Kitty 
Published: January 2015

AbstractBACKGROUNDRecently much research has been focused on the production and refinery of biobased fuels. The production of biofuels derived from lignocellulosic biomass is recognized as a promising route to produce biobased fuels responsibly. Often, product streams (e.g. glucose) still contain small amounts of undesired components (e.g. furans such as HMF). This study focuses on the removal of furans produced during the fermentation. In earlier work, styrene based resins have been identified as promising materials for this separation. In this work the kinetic properties of the most promising resin: DowexTM OptiporeTM L493 are studied.RESULTSThe diffusion coefficient of 5 mg L−1 HMF was ∼8 × 10−12 m2 s−1 in water and 3.0 × 10−12 m2 s−1 in a glucose solution. The reduced diffusion coefficient in the particle when glucose is present is caused by the higher viscosity of the glucose solution and it indicates that diffusion is controlled by surface and pore diffusion. The breakthrough curves of HMF on Optipore showed that the column is very efficient under conditions of interest.CONCLUSIONThis study shows that Optipore is a much more efficient resin for HMF removal than currently used resins. Its fast kinetics and capacity make it possible to efficiently remove HMF from glucose solutions. © 2015 Society of Chemical Industry
Kinetics and mechanisms of adsorption/desorption of the ionic liquid 1‐buthyl‐3‐methylimidazolium bromide into mordenite
Authors: Chanie Yewilsaw , Díaz Isabel , Pérez Eduardo 
Published: January 2015

AbstractBACKGROUNDKinetics of adsorption and desorption associated with diffusion mechanisms is of crucial importance to use zeolites as catalysts. In particular, diffusion of ionic liquids (IL) is of interest when using them as solvent for reactions or preparing composite IL–Zeolite. This work investigated the kinetics of adsorption/desorption of 1‐buthyl‐3‐methylimidazolium bromide ([BMIM]+Br−) into mordenite (MOR), a channel‐like zeolite.RESULTSThe adsorbed IL is characterized and quantified using infrared spectroscopy (FTIR), thermogravimetrical analysis (TGA) and CHN elemental analysis. The results show rapid adsorption of c. 0.7 mol of [BMIM]+Br− per gram of MOR but most of it remains in the pores of the zeolite upon desorption because only 9% is desorbed. CHN and TGA also suggest that adsorption of [BMIM]+Br− into the ammonium form of MOR can follow an ionic exchange mechanism. Experimental results can be fitted to a pseudo‐second‐order kinetic equation.CONCLUSIONThe kinetics and mechanisms of adsorption and desorption of [BMIM]+Br− into MOR have been studied. Adsorption kinetics is relatively quick; but only partial desorption occurs probably due to the strength of the [BMIM]+Br−–MOR interaction. The observed differences between the adsorption of [BMIM]+Br− into the different forms of MOR give important information on the interaction mechanisms. © 2015 Society of Chemical Industry
Coupled ARTP and ALE strategy to improve anaerobic cell growth and succinic acid production by Escherichia coli
Authors: Ma Jiang‐feng , Wu Ming‐ke , Zhang Chang‐qing , He Ai‐yong , Kong Xiang‐ping , Li Gan‐lu , Wei Ce , Jiang Min 
Published: January 2015

AbstractBACKGROUNDEscherichia coli AFP111, a facultative anaerobic strain, shows a very low cell growth and succinic acid production rate in minimal medium during exclusively anaerobic fermentation. Therefore, atmospheric and room temperature plasma (ARTP) and adaptive laboratory evolution (ALE) strategies were adopted to improve cell growth and succinic acid production under anaerobic conditions.RESULTSA mutant, BER208, was obtained by ARTP and ALE used successively. When cultured in a 3 L stirred bioreactor, the specific growth rate of BER208 was increased 3.12‐fold compared with AFP111. Also, it consumed 40 g L−1 glucose and produced 27.9 g L−1 succinic acid with a glucose utilization rate of 0.54 g L−1 h−1 and succinic acid productivity of 0.38 g L−1 h−1, which were 3.7‐fold and 2.5‐fold higher than those of the original strain. Further investigation showed that the activities of glucokinase and phosphoenolpyruvate carboxykinase (PCK) were increased 3.3‐fold and 16‐fold in the mutant compared with the original strain.CONCLUSIONThe combined strategy of ARTP and ALE was an effective approach to obtain mutants with improved performance. © 2015 Society of Chemical Industry
Insight on the fundamentals of advanced oxidation processes. Role and review of the determination methods of reactive oxygen species
Authors: Fernández‐Castro Pablo , Vallejo Marta , San Román Mª Fresnedo , Ortiz Inmaculada 
Published: January 2015

AbstractAdvanced oxidation processes (AOPs) have known increased application to treat wastewaters containing recalcitrant compounds that are hardly degraded by conventional technologies. AOPs are characterized by the formation of strong oxidants such as hydroxyl radicals, superoxide anions, hydroperoxyl radicals and singlet oxygen, which react with the contaminant, contributing to its degradation. This paper provides an overview of the determination methods of reactive oxygen species, ROS, in the application of AOPs; the methods developed in the available literature for the detection and quantification of ROS are reviewed as a first step in the assessment and detailed description of the mechanisms involved in the oxidation reactions, focusing on the critical analysis of the main strengths and weaknesses presented by the probe molecules employed in the evaluated studies. © 2015 Society of Chemical Industry
Effect of hydraulic retention time, temperature, and organic load on a horizontal subsurface flow constructed wetland treating cheese whey wastewater
Authors: Sultana Mar‐Yam , Mourti Christina , Tatoulis Triantafyllos , Akratos Christos S , Tekerlekopoulou Athanasia G , Vayenas Dimitrios V 
Published: January 2015

AbstractBACKGROUNDOne of the main components of dairy wastewaters is cheese whey. Although different technologies have been used extensively in the past for cheese‐whey treatment, constructed wetlands (CWs) applications are limited. Furthermore, the effect of crucial operational parameters (e.g. temperature, pollutant loading rate) have not been thoroughly studied. Having this in mind, two horizontal subsurface flow pilot‐scale CW units (one planted and one unplanted) were used to treat secondary cheese whey, in order to examine the effect of different chemical oxygen demand (COD) influent concentrations (1200 to 7200 mg L−1), hydraulic residence times (8, 4, 2 and 1 day) and temperature (2.4 to 32.9 °C).RESULTSDuring a 2‐year operating period both pilot‐scale units successfully removed organic matter, with COD removal efficiencies recorded at 91% and 77.2% for the planted and the unplanted unit, respectively. Hydraulic residence time affected COD removal efficiency only when limited to 1 day. Temperature significantly affected COD removal only in the unplanted unit.CONCLUSIONSConstructed wetlands could successfully treat secondary cheese whey and provide COD effluent concentrations below EU legislation, when hydraulic residence time is above 2 days and COD influent concentration ranges from 1200 to 3500 mg L−1. © 2015 Society of Chemical Industry