bluuebunny/crossref_metadata_2025_split huggingface dataset:
The landscape of cancer-associated transcript fusions in adult brain tumors: a longitudinal assessment in 140 patients with cerebral gliomas and brain metastases
Authors: Metellus Philippe , Camilla Clara , Bialecki Emilie , Beaufils Nathalie , Vellutini Christine , Pellegrino Eric , Tomasini Pascale , Ahluwalia Manmeet S. , Mansouri Alireza , Nanni Isabelle , Ouafik L’Houcine 
Published: July 2024

BackgroundOncogenic fusions of neurotrophic receptor tyrosine kinase NTRK1, NTRK2, or NTRK3 genes have been found in different types of solid tumors. The treatment of patients with TRK fusion cancer with a first-generation TRK inhibitor (such as larotrectinib or entrectinib) is associated with high response rates (&gt;75%), regardless of tumor histology and presence of metastases. Due to the efficacy of TRK inhibitor therapy of larotrectinib and entrectinib, it is clinically important to identify patients accurately and efficiently with TRK fusion cancer. In this retrospective study, we provide unique data on the incidence of oncogenic NTRK gene fusions in patients with brain metastases (BM) and gliomas.Methods140 samples fixed and paraffin-embedded tissue (FFPE) of adult patients (59 of gliomas [17 of WHO grade II, 20 of WHO grade III and 22 glioblastomas] and 81 of brain metastasis (BM) of different primary tumors) are analyzed. Identification of NTRK gene fusions is performed using next-generation sequencing (NGS) technology using Focus RNA assay kit (Thermo Fisher Scientific).ResultsWe identified an ETV6 (5)::NTRK3 (15) fusion event using targeted next-generation sequencing (NGS) in one of 59 glioma patient with oligodendroglioma–grade II, IDH-mutated and 1p19q co-deleted at incidence of 1.69%. Five additional patients harboring TMPRSS (2)::ERG (4) were identified in pancreatic carcinoma brain metastasis (BM), prostatic carcinoma BM, endometrium BM and oligodendroglioma (grade II), IDH-mutated and 1p19q co-deleted. A FGFR3 (17)::TACC3 (11) fusion was identified in one carcinoma breast BM. Aberrant splicing to produce EGFR exons 2-7 skipping mRNA, and MET exon 14 skipping mRNA were identified in glioblastoma and pancreas carcinoma BM, respectively.ConclusionsThis study provides data on the incidence of NTRK gene fusions in brain tumors, which could strongly support the relevance of innovative clinical trials with specific targeted therapies (larotrectinib, entrectinib) in this population of patients. FGFR3 (17)::TACC3 (11) rearrangement was detected in breast carcinoma BM with the possibility of using some specific targeted therapies and TMPRSS (2)::ERG (4) rearrangements occur in a subset of patients with, prostatic carcinoma BM, endometrium BM, and oligodendroglioma (grade II), IDH-mutated and 1p19q co-deleted, where there are yet no approved ERG-directed therapies.
Therapy with sirolimus in vascular anomalies: the experience of two Italian centers on 14 pediatric patients
Authors: Neirotti A. , Barat V. , Coppo P. , La Selva R. , Manicone R. , Cotti R. , Sensini M. , Mussa A. , Gatto M. , Farri F. , Basso M. E. , Fagioli F. 
Published: July 2024

IntroductionVascular anomalies (VAs) constitute a heterogeneous group of tumors and malformations capable of inducing significant clinical events in specific patients, such as the compression of vital organs, pain, functional impairment, or acquired coagulopathy. Molecular investigations into the underlying mechanisms of VAs have unveiled the frequent involvement of the PI3 K/AKT/mTOR pathway. Sirolimus, a specific mTOR inhibitor, has emerged as a potential therapeutic agent; however, its routine clinical application in complex VAs is currently restricted by a lack of extensive clinical experience.MethodsBetween 2015 and 2024, we administered sirolimus to 14 pediatric patients with various types of vascular anomalies in two Italian centers, subjecting them to clinical and instrumental follow-up to investigate its efficacy and the possible occurrence of adverse events.ResultsAn overall improvement in or stability of their vascular anomalies was reported by 86% of patients. We also assessed toxicity, noting a low prevalence of life-threatening adverse events: only one case of sepsis was reported in a patient with a severe clinical condition, and four cases of recurrent aphthosis (28%) were reported. The most common side effect was dyslipidemia, with 43% of patients developing hypercholesterolemia (21%) or hypertriglyceridemia (21%), although these patients generally did not reach severe levels.DiscussionIn line with data in the literature, according to our experience, medical therapy with sirolimus should be considered in pediatric patients affected by vascular anomalies.
Mapping the global research landscape on depression for patients with chronic kidney disease: a bibliometric and visualized analysis from 2006 to 2022
Authors: Xu Wenchao , Chen Zitong , Zhang Yurong , Zhao Jiayu , Huang Wenyong , Guo Xiao , Zhang Jianyu 
Published: July 2024

BackgroundChronic Kidney Disease (CKD), a complex and multifaceted health issue, significantly contributes to global mortality rates. Accompanying chronic conditions, depression notably exacerbates health outcomes, increasing both mortality risk and the burden on affected individuals. This study employs bibliometric and visual analytics to evaluate the evolution, current trends, and future research directions in the field of CKD and depression.MethodsWe conducted a thorough investigation using the Web of Science Core Collection, focusing on literature published from 2006 to 2022 that examines the interplay between CKD and depression. The analysis was enriched with bibliometric and visualization tools such as bibliometrix, CiteSpace, and VOSviewer to distill the essence of the research corpus.ResultsOur analysis incorporated 2,409 CKD-related publications, with significant contributions from the United States, China, and England. BMC Nephrology emerged as the leading publication outlet, while the American Journal of Kidney Diseases featured the most cited articles. Key terms such as “depression,” “quality-of-life,” “mortality,” “prevalence,” and “hemodialysis” dominated the keyword landscape, indicating the research focus areas.ConclusionThis bibliometric analysis offers an in-depth view of the research trajectory in CKD and depression. It provides valuable insights for researchers seeking relevant literature, potential collaborators, and an understanding of the field’s current hotspots and emerging frontiers. The findings of this study are instrumental in guiding and enriching future research endeavors in this domain.
Effects of salidroside on atherosclerosis: potential contribution of gut microbiota
Authors: Fei Si-Fan , Hou Can , Jia Fang 
Published: July 2024

Graphical AbstractThis review seeks to offer a comprehensive look at how salidroside impacts gut microbiota and its potential therapeutic role in treating atherosclerosis. (A) Salidroside has been shown to have a positive impact on atherosclerosis by promoting the growth of beneficial bacteria and decreasing the levels of harmful bacteria in the body. (B) Salidroside has been shown to enhance the integrity and function of the intestinal mucosal barrier through its ability to suppress NF-κB and p38 MAPK signaling pathways, modulate the NF-κB/MAPK/JAK-STAT3 signaling pathways and increase the expression of antimicrobial peptides HD-5 and HD-6. (C) Salidroside can reduce TMAO production through reducing the abundance of Firmicutes and Proteobacteria. (D) Salidroside can improve the expression of SCFAs, through increasing the abundance of some special bacteria. (E) Salidroside can reduce LPS-induced inflammation, which is associated with the inhibition of the ROS-mediated PI3K/AKT/mTOR signaling pathway, the downregulation of exosome miR-199a-5p, and the attenuation of the Notch-Hes signaling pathway. (F) Salidroside can inhibit NLRP3-associated gut-coronary axis, including TLR4/MyD88/NF-κB/NLRP3 signaling pathway, AMPK/NF-κB/NLRP3 signaling pathway, and P2X7/NF-κB/NLRP3 signaling pathway.
Efficacy, safety, and cost-effectiveness of pegylated PEG-rhg-CSF in pediatric patients receiving high-intensity chemotherapy: results from a phase II study
Authors: Huang Junting , Zhu Jia , Jiang Lian , Xu Jiaqian , Lin Xiheng , Chang Jian , Zhang Xiaohong , Lu Suying , Sun Feifei , Wang Juan , Que Yi , Ye Zhonglv , Yang Lihua , Yuan Xiuli , Cai Weisong , Tian Chuan , Wu Yanpeng , He Xiangling , Tang Yan-Lai , Zhang Yizhuo 
Published: July 2024

BackgroundHigh-intensity chemotherapy can cause life-threatening complications in pediatric patients. Therefore, this study investigated safety and efficacy of long-acting pegylated recombinant human granulocyte colony-stimulating factor (PEG-rhG-CSF; Jinyouli®) in children undergoing high-intensity chemotherapy.MethodsTreatment-naive patients received post-chemotherapy PEG-rhG-CSF as primary prophylaxis for two cycles. The primary endpoints were drug-related adverse events (AEs) and bone pain scores. Secondary endpoints included grade 3–4 neutropenia, duration of neutropenia recovery, absolute neutrophil count changes, febrile neutropenia (FN), reduced chemotherapy intensity, antibiotic usage, and AE severity. The cost-effectiveness of PEG-rhG-CSF was compared with that of rhG-CSF (Ruibai®).ResultsHere, 307 and 288 patients underwent one and two PEG-rhG-CSF cycles, respectively. Ninety-one patients experienced drug-related AEs, primarily bone pain (12.7%). Moreover, Grade 3–4 neutropenia and FN were observed. Median FN durations were 3.0 days in both cycles. No drug-related delays were observed during chemotherapy. One patient experienced grade 4 neutropenia-induced reduction in chemotherapy intensity during cycle 2. In total, 138 patients received antibiotics. PEG-rhG-CSF exhibited superior cost-effectiveness compared to rhG-CSF.ConclusionOur findings indicate that PEG-rhG-CSF is safe, efficient, and cost-effective in pediatric patients undergoing high-intensity chemotherapy, providing preliminary evidence warranting further randomized controlled trials.
Formation mechanism and regulation analysis of trumpet leaf in Ginkgo biloba L
Authors: Li Xin-hui , Kang Xiao-jing , Zhang Xin-yue , Su Li-ning , Bi Xing , Wang Rui-long , Xing Shi-yan , Sun Li-min 
Published: July 2024

IntroductionThe research on plant leaf morphology is of great significance for understanding the development and evolution of plant organ morphology. As a relict plant, the G. biloba leaf morphology typically exhibits bifoliate and peltate forms. However, throughout its long evolutionary history, Ginkgo leaves have undergone diverse changes.MethodsThis study focuses on the distinct “trumpet” leaves and normal fan-shaped leaves of G. biloba for analysis of their phenotypes, photosynthetic activity, anatomical observations, as well as transcriptomic and metabolomic analyses.ResultsThe results showed that trumpet-shaped G. biloba leaves have fewer cells, significant morphological differences between dorsal and abaxial epidermal cells, leading to a significantly lower net photosynthetic rate. Additionally, this study found that endogenous plant hormones such as GA, auxin, and JA as well as metabolites such as flavonoids and phenolic acids play roles in the formation of trumpet-shaped G. biloba leaves. Moreover, the experiments revealed the regulatory mechanisms of various key biological processes and gene expressions in the trumpet-shaped leaves of G. biloba.DiscussionDifferences in the dorsal and abdominal cells of G. biloba leaves can cause the leaf to curl, thus reducing the overall photosynthetic efficiency of the leaves. However, the morphology of plant leaves is determined during the primordia leaf stage. In the early stages of leaf development, the shoot apical meristem (SAM) determines the developmental morphology of dicotyledonous plant leaves. This process involves the activity of multiple gene families and small RNAs. The establishment of leaf morphology is complexly regulated by various endogenous hormones, including the effect of auxin on cell walls. Additionally, changes in intracellular ion concentrations, such as fluctuations in Ca2+ concentration, also affect cell wall rigidity, thereby influencing leaf growth morphology.
Pilot study on the effectiveness of the socialmind program for the rehabilitation of social cognition following acquired brain injury
Authors: Rivas-García Sandra , García-Bermúdez Olga , Catena Andrés , Caracuel Alfonso 
Published: July 2024

BackgroundPeople with acquired brain injury (ABI) often have Social Cognition (SC) deficits. Impairment of SC causes the individual to have difficulties in daily functioning and can lead to social isolation. Research aimed at rehabilitation of SC in individuals with ABI is scarce and almost always addresses only one component of this ability.ObjectiveThis pilot study aimed to assess the effectiveness of the new “SocialMind” program in improving all core components of SC in people with ABI.MethodThe study included 31 participants with ABI, divided into experimental and control groups. The study spanned 44 weeks, involving an initial meeting, evaluation, training, and final assessment phases. The SocialMind program, structured into four modules, each with a duration of 30 h, targeted each SC component through tailored exercises. The program addressed emotion recognition, social awareness, ToM, and empathy.ResultsThe SocialMind group demonstrated significant improvements in emotion recognition (p = 0.017), social knowledge (p &lt; 0.001), and empathy (p = 0.001) compared to the control group. ToM also showed a notable improvement that approached significance (p = 0.057).ConclusionThis pilot study suggests that the SocialMind program effectively enhances three of the four core components of SC in individuals with ABI.
Association of household food insecurity with sociodemographic factors and obesity in US youth: findings from the National Health and Nutrition Examination Survey 2017–2018
Authors: Mokari-Yamchi Amin , Faghfouri Amir Hossein , Gholami Samira , Nattagh-Eshtivani Elyas , Gheibi Shahsanam 
Published: July 2024

BackgroundThe objective is to determine the prevalence of household food insecurity (HFI) based on sociodemographic factors and their relationship to obesity in youth.MethodsThe study included a sample of 1,962 youth (aged 6–18) from the National Health and Nutrition Examination Survey (NHANES). The US Household Food Security Survey Module is used to measure food security over the past 12 months. Logistic regression models were used to estimate adjusted odds ratios (ORs) while controlling for covariates.ResultsIn total, 27.4% of the individuals surveyed experienced HFI. Youth from food insecure households were more likely to be obese (adjusted odds ratio [aOR]: 1.59 [95% confidence interval: 1.19–2.13]) and also having abdominal obesity (aOR: 1.56 [95% CI: 1.19–2.03]). however, factors such as non-Hispanic ethnicity, having a Head of household with a college degree, and households with an income exceeding 350% of the poverty line were associated with a reduced risk of facing HFI.ConclusionHispanic individuals, households with lower parental education levels, and lower family incomes, are disproportionately affected by food insecurity. Furthermore, HFI has been associated with an increased risk of overweight and abdominal obesity among youth. Addressing FI requires targeted policies and interventions that prioritize vulnerable groups.
Analysis of epidemiological characteristics of extrapulmonary tuberculosis from South-Central China
Authors: Yu Yanyan , Xiang Yu , Liu Haican , Yang Shuliu , Li Machao , Liu Binbin , Xu Da , Wu Yaning , Li Wenbin , Fang Tanwei , Li Jixiang , Xu Donglei , Wan Kanglin , Tan Yunhong , Yuan Xiuqin , Li Guilian 
Published: July 2024

ObjectivesThis study aimed to investigate the epidemiological and drug resistance (DR) characteristics of extrapulmonary tuberculosis (EPTB) in South-Central China.MethodsEPTB inpatients who were culture-positive for Mycobacterium tuberculosis were retrospectively included in a study at a provincial TB hospital in Hunan, a province in South-Central China, from January 2013 to December 2021. Demographic, clinical, and drug susceptibility data were retrieved from TB treatment records. Descriptive statistical methods and a Chi-squared test were used to analyze the epidemiological and DR characteristics of EPTB patients. A logistic regression model was used to explore the risk factors of rifampicin-resistant/multidrug-resistant (RR/MDR)-EPTB.ResultsA total of 1,324 cases were included. The majority of EPTB patients were in the age range of 20–29 years, were predominantly men (male-to-female ratio: 2.03), and were farmers (65.63%). Most EPTB cases were found in 2013 and 2017 from 2013 to 2021. The most prevalent subtypes of EPTB were lymphatic TB (29.83%, 395/1,324), multiple EPTB (20.85%, 276/1,324), and musculoskeletal TB (14.65%, 194/1,324). Musculoskeletal TB and genitourinary TB predominantly presented as exclusive EPTB forms, while lymphatic TB and pharyngeal/laryngeal TB often co-occurred with pulmonary TB (PTB). Drug susceptibility testing results showed that total DR rates (resistance to any of RFP, isoniazid [INH], streptomycin [STR], and/or ethambutol [EMB]) and RR/MDR rates in EPTB were 25.23% and 12.39%, respectively. Musculoskeletal TB exhibited the highest rates of total DR (31.40%), INH resistance (28.90%), STR resistance (20.10%), EMB resistance (6.20%), MDR (13.90%), and poly-DR (6.70%). The multivariable logistic regression model showed that patients aged from 20 to 59 years (compared to those aged 10 years), workers (compared to retirees), and EPTB patients from the south and west of Hunan (compared to those from the east of Hunan) were at an increased risk of developing RR/MDR EPTB (all OR values &gt; 1).ConclusionOur study provided a detailed account of the epidemiological and DR characteristics of EPTB in Hunan province, China. The significant DR rates, particularly in musculoskeletal TB cases, highlight the need for timely diagnosis, effective drug susceptibility testing, and the development of more effective treatment regimens for EPTB, especially targeting musculoskeletal TB treatments.
An international survey on canine urinary incontinence: case frequency, diagnosis, treatment and follow-up
Authors: Falceto M. V. , Caccamo R. , Garrido A. M. , Pisu M. C. , Tejedor M. T. , Trerotoli P. , Nicoli S. , Zagarella P. , Lippi I. , García-Pedraza E. , Rambaldi J. , Kirilova D. , Mitjana O. 
Published: July 2024

IntroductionUrinary incontinence (UI) consists of involuntary leakage of urine during the storage phase of urination.MethodsAn anonymous survey was given to Spanish and Italian veterinarians about canine UI treated cases, diagnosis, treatment, follow-up, and professional interest.Results and discussionMost veterinarians treated ≤3 cases/quarter, resulting in the percentage of incontinence males being lower than that of females (1-4% vs 0-24%). The percentage of spayed incontinent females was lower in Spain (0-24%) than in Italy (75-100%). Most diagnoses were based on a diagnostic algorithm (Spain: 88.7%; Italy: 65.3%); patient report and history, blood work, urinalysis and abdominal ultrasound. Urethral/bladder pressure measurement was unusual (Spain: 0.2%; Italy: 2.4%). In Spain, radiology with contrast medium and CT urography (26.3% and 34.4%, respectively) were more frequent than in Italy (11.6% and 22.7%, respectively). When suspecting urethral sphincter mechanism incompetence pharmacological trial (Spain: 93.2%; Italy: 78.9%). The first-choice medical treatment was Phenylpropanolamine, followed by Ephedrine and Deslorelin. When pharmacotherapy failed, the most frequent option was drug change, followed by increased drug dosage/frequency of administration, surgical therapy and colposuspension. A review was completed after the first week of treatment followed by periodic reviews. Most of the respondents participated in continuing education only if UI occurred in their everyday practice (Spain: 63.0%; Italy: 55.4%) and about 30% responders did it regardless of the number of UI cases treated (Spain: 30.5%; Italy: 37.4%).ConclusionSome recommendations in clinical practice were made. UI can be underestimated by owners; therefore, a complete history should be obtained by veterinarians. Veterinarians should carefully evaluate if spaying is advisable considering it could increase UI risk. A step-by-step approach is recommended and a specific diagnostic-therapeutic algorithm for UI in dogs is provided. Conservative approaches (regular exercise, weight loss in overweight dogs and observing an “incontinence diary” to identify abnormal patterns of urination) are advisable.
DEVELOPMENT OF HPLC-PDA METHOD FOR QUANTIFYING AMLODIPINE BESYLATE IN TABLETS
Authors: Thach Pha Thi Bo , Do Danh Nguyen Cong , Lam Kieu Thi Thuy 
Published: July 2024

Cardiovascular disease is a dangerous disease known to be the leading cause of death globally, and hypertension is considered one of the main risk factors contributing to the disease. Among the high blood pressure medications currently on the market, amlodipine besylate is a commonly used drug to treat high blood pressure and prevent related diseases. This study aims to develop a procedure for the quantification of amlodipine besylate in tablets and drug quality control methods, using highperformance liquid chromatography. The method used in this research was high-performance liquid chromatography (HPLC) combined with a photodiode aray detector (PDA) and then was subsequently evaluated according to the guidelines of the International Conference on Harmonization (ICH). Research results successfully developed a process for quantifying amlodipine besylate in tablets with specificity, linearity with a linear range from 8 to 32 µg/mL, high accuracy, high precision, and a short analysis time (5 minutes) that meets the requirements of thequantification process in drug testing and can be applied to check the quality of amlodipine drugs on the market.
-- PDF Article: https://drive.google.com/file/d/1P-TG0M9kxJlpiyh5-uknBaZsyhkOMeWf/view?usp=sharing
OVERVIEW OF THE CANINE PARVOVIRUS TYPE 2: ETIOLOGY, PATHOGENESIS, DIAGNOSIS, TREATMENT, AND PREVENTION
Authors: Nguyen Linh Thi Yen , Danh Ut 
Published: July 2024

Parvo in dogs is caused by canine parvovirus type 2 (CPV-2), a highly contagious viral disease in dogs that often causes high mortality and is mainly transmitted through the gastrointestinal tract. Nowadays, the variants identified include CPV-2a, CPV-2b, and CPV-2c. This pathogen has appeared in most countries around the world, including Vietnam. Its host range varies with each type of variant, and its relative frequency and genetic variability also vary between countries. The research provides an overview of key information regarding themolecular structure, origin, evolution, and pathophysiology of CPV-2, drawing on 30 published study results from various countries worldwide. This paper helps to identify risk factors that contribute to CPV-2 disease. In addition, the research also synthesizes research on advanced diagnosis, treatment, and CPV-2 vaccine development, serving as a basis for veterinarians, veterinary students, and breeders to control and limit the impact of upper enteritis caused by CPV-2.
-- PDF Article: https://drive.google.com/file/d/1ah4LKB4Hr1V1BiXSzSgKTinyDLei77RJ/view?usp=sharing