bluuebunny/crossref_metadata_2025_split huggingface dataset:
The iteration deficit in responsive regulation: Are regulatory ambassadors an answer?
Authors: Braithwaite John , Hong Seung‐Hun 
Published: January 2014

AbstractOne reason that regulation is difficult is that repeated encounters between regulator and regulatee are rare. We suggest diplomacy as a model for reconfiguring regulatory institutions in response. Ambassadors for Regulatory Affairs who would be agents for all state regulatory agencies could be based in most large firms and small and medium enterprises that pose unusual regulatory risks. In rural towns, police would be trained as regulatory ambassadors. Just as aUSSecretary of State can launch a “diplomatic surge” inMyanmar from 2009, so regulatory surges are possible in market sectors of high risk or high opportunity. We propose strategies of indirect reciprocity as a way in which reciprocity that is only episodic in these strategic ways can promote more general responsiveness. Indirect reciprocity is reciprocity that we do not personally experience, but learn from the experience of a culture. This means that so long as we sustain regulation as a relational as opposed to a purely technocratic process, indirect reciprocity might civilize regulatory compliance in an historical process informed by the theories ofElias andPutnam.
Gender differences of cognitive function in migraine patients: evidence from event-related potentials using the oddball paradigm
Authors: Wang Rongfei , Dong Zhao , Chen Xiaoyan , Zhang Mingjie , Yang Fan , Zhang Xiaolan , Jia Weiquan , Yu Shengyuan 
Published: January 2014

Abstract

Background
Migraine shows gender-specific incidence and has a higher prevalence in females. Gender plays an important role in the prevalence of migraine, but few studies have investigated the effect of gender on the cognitive functions of migraine patients. This study investigated gender differences in the cognitive function of migraine patients without aura.

Methods
We recruited 29 migraine patients (15 females; mean age 25.4 y) during the interictal period and 28 healthy age-matched participants (14 females; mean age 24.8 y). We used an auditory oddball paradigm to analyze target processing using event-related potentials.

Results
We investigated the N2 and P3 components. The P3 amplitude was decreased in patients compared with the control, and this reduction was not modulated by gender. These results of the P3 provided a new evidence for the dysfunction of cognitive function in migraine patients. The N2 amplitude was larger for male than female migraine patients, and this gender effect was not found in the control group.

Conclusions
These results of the P3 provided a new evidence for the dysfunction of cognitive function in migraine patients. And those of N2 may explain that male patients have the super-sensitivity of cerebral function relevant to the early target-selection and response preparation. Our findings emphasize the importance of considering gender when researching the cognitive function of migraine patients.
Inferring the relation between transcriptional and posttranscriptional regulation from expression compendia
Authors: Ishchukov Ivan , Wu Yan , Van Puyvelde Sandra , Vanderleyden Jos , Marchal Kathleen 
Published: January 2014

AbstractBackgroundPublicly available expression compendia that measure both mRNAs and sRNAs provide a promising resource to simultaneously infer the transcriptional and the posttranscriptional network. To maximally exploit the information contained in such compendia, we propose an analysis flow that combines publicly available expression compendia and sequence-based predictions to infer novel sRNA-target interactions and to reconstruct the relation between the sRNA and the transcriptional network.ResultsWe relied on module inference to construct modules of coexpressed genes (sRNAs). TFs and sRNAs were assigned to these modules using the state-of-the-art inference techniques LeMoNe and Context Likelihood of Relatedness (CLR). Combining these expressions with sequence-based sRNA-target interactions allowed us to predict 30 novel sRNA-target interactions comprising 14 sRNAs. Our results highlight the role of the posttranscriptional network in finetuning the transcriptional regulation, e.g. by intra-operonic regulation.ConclusionIn this work we show how strategies that combine expression information with sequence-based predictions can help unveiling the intricate interaction between the transcriptional and the posttranscriptional network in prokaryotic model systems.
Interaction between forest biodiversity and people’s use of forest resources in Roviana, Solomon Islands: implications for biocultural conservation under socioeconomic changes
Authors: Furusawa Takuro , Sirikolo Myknee Qusa , Sasaoka Masatoshi , Ohtsuka Ryutaro 
Published: January 2014

AbstractBackgroundIn Solomon Islands, forests have provided people with ecological services while being affected by human use and protection. This study used a quantitative ethnobotanical analysis to explore the society–forest interaction and its transformation in Roviana, Solomon Islands. We compared local plant and land uses between a rural village and urbanized village. Special attention was paid to how local people depend on biodiversity and how traditional human modifications of forest contribute to biodiversity conservation.MethodsAfter defining locally recognized land-use classes, vegetation surveys were conducted in seven forest classes. For detailed observations of daily plant uses, 15 and 17 households were randomly selected in the rural and urban villages, respectively. We quantitatively documented the plant species that were used as food, medicine, building materials, and tools.ResultsThe vegetation survey revealed that each local forest class represented a different vegetative community with relatively low similarity between communities. Although commercial logging operations and agriculture were both prohibited in the customary nature reserve, local people were allowed to cut down trees for their personal use and to take several types of non-timber forest products. Useful trees were found at high frequencies in the barrier island’s primary forest (68.4%) and the main island’s reserve (68.3%). Various useful tree species were found only in the reserve forest and seldom available in the urban village. In the rural village, customary governance and control over the use of forest resources by the local people still functioned.ConclusionsHuman modifications of the forest created unique vegetation communities, thus increasing biodiversity overall. Each type of forest had different species that varied in their levels of importance to the local subsistence lifestyle, and the villagers’ behaviors, such as respect for forest reserves and the semidomestication of some species, contributed to conserving diversity. Urbanization threatened this human–forest interaction. Although the status of biodiversity in human-modified landscapes is not fully understood, this study suggested that traditional human modifications can positively affect biodiversity and that conservation programs should incorporate traditional uses of landscapes to be successful.
An ethnobiological study in Kala Chitta hills of Pothwar region, Pakistan: multinomial logit specification
Authors: Arshad Muhammad , Ahmad Mushtaq , Ahmed Ejaz , Saboor Abdul , Abbas Azhar , Sadiq Shumaila 
Published: January 2014

Abstract

Background
This paper constitutes an important ethnobiological survey in the context of utilizing biological resources by residents of Kala Chitta hills of Pothwar region, Pakistan. The fundamental aim of this research endeavour was to catalogue and analyse the indigenous knowledge of native community about plants and animals. The study is distinctive in the sense to explore both ethnobotanical and ethnozoological aspects of indigenous culture, and exhibits novelty, being based on empirical approach of Multinomial Logit Specifications (MLS) for examining ethnobotanical and ethnozoological uses of specific plants and animals.

Methods
To document the ethnobiological knowledge, the survey was conducted during 2011–12 by employing a semi-structured questionnaire and thus 54 informants were interviewed. Plant and animal specimens were collected, photographed and properly identified. Distribution of plants and animals were explored by descriptive and graphical examination. MLS were further incorporated to identify the probability of occurrence of diversified utilization of plants and animals in multipurpose domains.

Results
Traditional uses of 91 plant and 65 animal species were reported. Data analysis revealed more medicinal use of plants and animals than all other use categories. MLS findings are also in line with these proportional configurations. They reveal that medicinal and food consumption of underground and perennial plants was more as compared to aerial and annual categories of plants. Likewise, medicinal utilization of wild animals and domestic animals were more commonly observed as food items. However, invertebrates are more in the domain of medicinal and food utilization. Also carnivores are fairly common in the use of medicine while herbivores are in the category of food consumption.

Conclusion
This study empirically scans a good chunk of ethnobiological knowledge and depicts its strong connection with indigenous traditions. It is important to make local residents beware of conservation status of species and authentication of this knowledge needs to be done in near future. Moreover, Statistically significant findings impart novelty in the existing literature in the field of ethnobiology. Future conservation, phytochemical and pharmacological studies are recommended on these identified plants and animals in order to use them in a more sustainable and effective way.
A sheeppox outbreak in Morocco: isolation and identification of virus responsible for the new clinical form of disease
Authors: Zro Khalil , Zakham Fathiah , Melloul Marouane , El Fahime Elmostafa , Ennaji Moulay Mustapha 
Published: January 2014

Abstract

Background
Sheeppoxvirus (SPPV) is a member of the Capripoxvirus genus of the Poxviridae family, which causes significant economic losses in Morocco. The resurgence of the sheeppox disease during 2010 was characterized by an emergence of a classical nodular form for the first time in Morocco. However, little is known about the virus strain responsible for nodular form. In this study, thirty three sheep, from the eastern region of Morocco, clinically infected were examined and dead animals were autopsied.
A rapid diagnostic assay for SPPV using different type of clinical samples would be useful for outbreak management. The aim of this work was to isolate the virus strain responsible for nodular form and we identified and compared by phylogenetic analysis the field strain with Moroccan vaccine strain targeting the thymidine kinase (TK) gene and the chemokine analogue receptor of interleukin (IL8) gene. Further, it was important to investigate and validate a real-time PCR using different clinical and post-mortem samples to manage epidemic sheeppox disease.

Results
The nodular form of sheeppox disease observed in Morocco was clinically characterized by fever, depression, lacrimation, diarrhea in lambs and nodule. At necropsy, the most affected organ was the lung. The etiological strain was successfully isolated from lung nodule in a dead lamb and was identified by using real-time PCR that has been tested and validated on different types of clinical and post mortem samples from naturally infected animals. Sequence and phylogenetic analysis of TK and IL8 gene showed that there was a very close relationship between field and vaccine strain. They were clustered within other SPPV strains.

Conclusion
In the current study, we show for the first time the nodular form of sheeppox in Morocco. We demonstrate a robust real-time PCR-based diagnostic assay to detect the sheeppox virus in multiple sample that can be implemented to efficiently manage the disease outbreak. Our study also offers the prospect for future molecular studies to understand the clinical forms.
Design and analysis of LacI-repressed promoters and DNA-looping in a cyanobacterium
Authors: Camsund Daniel , Heidorn Thorsten , Lindblad Peter 
Published: January 2014

Abstract

Background
Cyanobacteria are solar-powered prokaryotes useful for sustainable production of valuable molecules, but orthogonal and regulated promoters are lacking. The Lac repressor (LacI) from Escherichia coli is a well-studied transcription factor that is orthogonal to cyanobacteria and represses transcription by binding a primary lac operator (lacO), blocking RNA-polymerase. Repression can be enhanced through DNA-looping, when a LacI-tetramer binds two spatially separated lacO and loops the DNA. Ptrc is a commonly used LacI-repressed promoter that is inefficiently repressed in the cyanobacterium Synechocystis PCC 6803. Ptrc 2O, a version of Ptrc with two lacO, is more efficiently repressed, indicating DNA-looping. To investigate the inefficient repression of Ptrc and cyanobacterial DNA-looping, we designed a Ptrc-derived promoter library consisting of single lacO promoters, including a version of Ptrc with a stronger lacO (Ptrc1O-proximal), and dual lacO promoters with varying inter-lacO distances (the Ptrc 2O-library).

Results
We first characterized artificial constitutive promoters and used one for engineering a LacI- expressing strain of Synechocystis. Using this strain, we observed that Ptrc 1O-proximal is similar to Ptrc in being inefficiently repressed. Further, the Ptrc 2O-library displays a periodic repression pattern that remains for both non- and induced conditions and decreases with longer inter-lacO distances, in both E. coli and Synechocystis. Repression of Ptrc 2O-library promoters with operators out of phase is less efficient in Synechocystis than in E. coli, whereas repression of promoters with lacO in phase is efficient even under induced conditions in Synechocystis. Two well-repressed Ptrc 2O promoters were highly active when tested in absence of LacI in Synechocystis.

Conclusions
The artificial constitutive promoters herein characterized can be utilized for expression in cyanobacteria, as demonstrated for LacI. The inefficient repression of Ptrc and Ptrc 1O-proximal in Synechocystis, as compared to E. coli, may be due to insufficient LacI expression, or differences in RNAP subunits. DNA-looping works as a transcriptional regulation mechanism similarly as in E. coli. DNA-looping contributes strongly to Ptrc 2O-library repression in Synechocystis, even though they contain the weakly-repressed primary lacO of Ptrc 1O-proximal and relatively low levels of LacI/cell. Hence, Synechocystis RNAP may be more sensitive to DNA-looping than E. coli RNAP, and/or the chromatin torsion resistance could be lower. Two strong and highly repressed Ptrc 2O promoters could be used without induction, or together with an unstable LacI.
Relationship between humoral response against hepatitis C virus and disease overcome
Authors: Brakha Carine , Arvers Philippe , Villiers Florent , Marlu Alice , Buhot Arnaud , Livache Thierry , Calemczuk Roberto , Zarski Jean-Pierre , Villiers Christian L , Marche Patrice N , Villiers Marie-Bernadette 
Published: January 2014

Abstract

Conclusion

Humoral response against hepatitis C virus linear epitopes is partly modified according to the disease state. This study highlights the importance of considering relative quantities of antibodies with different specificities rather than the amount of each antibody.
Hepatitis C virus infection leads to liver disease whose severity can range from mild to serious lifelong illness. However the parameters involved in the evolution of the disease are still unknown. Among other factors, the virus-elicited antibody profile is suspected to play a role in the outcome of the disease. Analysis of the relationship between anti-virus antibodies and disease state requires the analysis of a large number of serums from patients (hepatitis C virus+) and of epitopes from the viral proteins. Such a study would benefit from microarray-based screening systems that are appropriate for high-throughput assays.
We used a method combining peptide chips and surface plasmon resonance imaging previously shown to be suitable for analyzing complex mediums and detecting peptide-protein interactions. 56 peptides covering the entire viral proteome were grafted on chips and their interaction with antibodies present in the 68 injected serums from infected and non-infected donors was measured. Statistical analyses were conducted to determine a possible relationship between antibodies (specificity and amount) and disease states.
A good discrimination between infected and non-infected donors validated our approach, and several correlations between antibodies profiles and clinical parameters have been identified. In particular, we demonstrated that ratios between particular antibodies levels allow for accurate discrimination of patients according to their pathologic states.
Study protocol for examining job strain as a risk factor for severe unipolar depression in an individual participant meta-analysis of 14 European cohorts
Authors:   IPD-Work Consortium, Madsen Ida E. H. , Hannerz Harald , Nyberg Solja T. , Magnusson Hanson Linda L. , Ahola Kirsi , Alfredsson Lars , Batty G. David , Bjorner Jakob B. , Borritz Marianne , Burr Hermann , Dragano Nico , Ferrie Jane E. , Hamer Mark , Jokela Markus , Knutsson Anders , Koskenvuo Markku , Koskinen Aki , Leineweber Constanze , Nielsen Martin L. , Nordin Maria , Oksanen Tuula , Pejtersen Jan H. , Pentti Jaana , Salo Paula , Singh-Manoux Archana , Suominen Sakari , Theorell Töres , Toppinen-Tanner Salla , Vahtera Jussi , Väänänen Ari , Westerholm Peter J. M , Westerlund Hugo , Fransson Eleonor , Heikkilä Katriina , Virtanen Marianna , Rugulies Reiner , Kivimäki Mika 
Published: January 2014

Background: Previous studies have shown that gainfully employed individuals with high work demands and low control at work (denoted “job strain”) are at increased risk of common mental disorders, including depression. Most existing studies have, however, measured depression using self-rated symptom scales that do not necessarily correspond to clinically diagnosed depression. In addition, a meta-analysis from 2008 indicated publication bias in the field.Methods: This study protocol describes the planned design and analyses of an individual participant data meta-analysis, to examine whether job strain is associated with an increased risk of clinically diagnosed unipolar depression based on hospital treatment registers. The study will be based on data from approximately 120,000 individuals who participated in 14 studies on work environment and health in 4 European countries. The self-reported working conditions data will be merged with national registers on psychiatric hospital treatment, primarily hospital admissions. Study-specific risk estimates for the association between job strain and depression will be calculated using Cox regressions. The study-specific risk estimates will be pooled using random effects meta-analysis.Discussion: The planned analyses will help clarify whether job strain is associated with an increased risk of clinically diagnosed unipolar depression. As the analysis is based on pre-planned study protocols and an individual participant data meta-analysis, the pooled risk estimates will not be influenced by selective reporting and publication bias. However, the results of the planned study may only pertain to severe cases of unipolar depression, because of the outcome measure applied.
Seed evolution: parental conflicts in a multi-generational household
Authors: Pires Nuno D. 
Published: January 2014

AbstractSeeds are multi-generational structures containing a small embryonic plant enclosed in layers of diverse parental origins. The evolution of seeds was a pinnacle in an evolutionary trend towards a progressive retention of embryos and gametes within parental tissue. This strategy, which dates back to the first land plants, allowed an increased protection and nourishing of the developing embryo. Flowering plants took parental control one step further with the evolution of a biparental endosperm that derives from a second parallel fertilization event. The endosperm directly nourishes the developing embryo and allows not only the maternal genes, but also paternal genes, to play an active role during seed development. The appearance of an endosperm set the conditions for the manifestation of conflicts of interest between maternal and paternal genomes over the allocation of resources to the developing embryos. As a consequence, a dynamic balance was established between maternal and paternal gene dosage in the endosperm, and maintaining a correct balance became essential to ensure a correct seed development. This balance was achieved in part by changes in the genetic constitution of the endosperm and through epigenetic mechanisms that allow a differential expression of alleles depending on their parental origin. This review discusses the evolutionary steps that resulted in the appearance of seeds and endosperm, and the epigenetic and genetic mechanisms that allow a harmonious coinhabitance of multiple generations within a single seed.
Pseudo-parabolic regularization of forward-backward parabolic equations: Power-type nonlinearities
Authors: Bertsch Michiel , Smarrazzo Flavia , Tesei Alberto 
Published: January 2014

Abstract

We study a quasilinear parabolic equation of
forward-backward type, under assumptions on the
nonlinearity which hold for a wide class of mathematical models, using a
pseudo-parabolic regularization of power type. We prove existence and uniqueness
of positive solutions of the regularized problem in a space of Radon
measures. It is shown that these solutions satisfy suitable entropy inequalities.
We also study their qualitative properties, in
particular proving that the singular part of the solution with respect to the Lebesgue measure
is constant in time.
Analgesic, anti-inflammatory and antipyretic effects of Ixora coccinea
Authors: Adnan Md. Syed Ali , Al-Amin Md. Mamun , Nasir Uddin Mir Muhammad , Shohel M. , Bhattacharjee Rajib , Hannan J.M.A. , Das Biplab Kumar 
Published: January 2014

Abstract

Background: The present study was carried out to explore the potential of the ethanol extract of Ixora coccinea L. (IC) leaves as analgesic, anti-inflammatory and antipyretic agents using the hot-plate, acetic acid-induced writhing, carrageenan-induced paw edema and brewer’s yeast-induced pyrexia tests in rodents.

Methods: The extract was prepared by soaking the dried powdered leaves of IC in ethanol for 2 days. The filtrate thus obtained by filtration and evaporation was considered as a stock solution and was used in all experimental models.

Results: Oral administration of IC (250 and 500 mg/kg) significantly (p<0.05) increased the reaction time in the hot-plate test. Ixora coccinea (250 and 500 mg/kg) produced 56.14% and 63.16% inhibition (p<0.05) in acetic acid-induced writhing. It also (250 and 500 mg/kg) produced significant (p<0.05) inhibition of paw edema pronounced at 6 h after carrageenan injection. Intraperitoneal administration of IC (250 and 500 mg/kg) lowered the body temperature in brewer’s yeast-induced hyperthermia.

Conclusions: Based on the findings, it may be concluded that the IC leaves possessed analgesic, anti-inflammatory, and antipyretic activities. Phytochemical constituents of IC leaves such as flavonoids, tannins, and triterpenes in ethanol extract could be correlated with its observed biological activities.