bluuebunny/crossref_metadata_2025_split huggingface dataset:
Validation of a mobility item bank for older patients in primary care
Authors: Cabrero-García Julio , Ramos-Pichardo Juan Diego , Muñoz-Mendoza Carmen Luz , Cabañero-Martínez María José , González-Llopis Lorena , Reig-Ferrer Abilio 
Published: December 2012

AbstractBackgroundTo develop and validate an item bank to measure mobility in older people in primary care and to analyse differential item functioning (DIF) and differential bundle functioning (DBF) by sex.MethodsA pool of 48 mobility items was administered by interview to 593 older people attending primary health care practices. The pool contained four domains based on the International Classification of Functioning: changing and maintaining body position, carrying, lifting and pushing, walking and going up and down stairs.ResultsThe Late Life Mobility item bank consisted of 35 items, and measured with a reliability of 0.90 or more across the full spectrum of mobility, except at the higher end of better functioning. No evidence was found of non-uniform DIF but uniform DIF was observed, mainly for items in the changing and maintaining body position and carrying, lifting and pushing domains. The walking domain did not display DBF, but the other three domains did, principally the carrying, lifting and pushing items.ConclusionsDuring the design and validation of an item bank to measure mobility in older people, we found that strength (carrying, lifting and pushing) items formed a secondary dimension that produced DBF. More research is needed to determine how best to include strength items in a mobility measure, or whether it would be more appropriate to design separate measures for each construct.
Establishment of a method to determine the magnetic particles in mouse tissues
Authors: Wu Yifan , Zhang Wuxu , Wang Yuxia , Li Qian , Gao Guo , Dong Na , Hu Hengyao , Wang Kan , Wu Junhua , Gao Zhongcai , Cui Daxiang 
Published: December 2012

Abstract
This work is aimed to evaluate a method to detect the residual magnetic nanoparticles (MNPs) in animal tissues. Ferric ions released from MNPs through acidification with hydrochloric acid can be measured by complexation with potassium thiocyanate. MNPs in saline could be well detected by this chemical colorimetric method, whereas the detected sensitivity decreased significantly when MNPs were mixed with mouse tissue homogenates. In order to check the MNPs in animal tissues accurately, three improvements have been made. Firstly, proteinase K was used to digest the proteins that might bind with iron, and secondly, ferrosoferric oxide (Fe3O4) was collected by a magnetic field which could capture MNPs and leave the bio-iron in the supernatant. Finally, the collected MNPs were carbonized in the muffle furnace at 420°C before acidification to ruin the groups that might bind with ferric ions such as porphyrin. Using this method, MNPs in animal tissues could be well measured while avoiding the disturbance of endogenous iron and iron-binding groups.
Co-delivery of docetaxel and endostatin by a biodegradable nanoparticle for the synergistic treatment of cervical cancer
Authors: Qiu Bo , Ji Minghui , Song Xiaosong , Zhu Yongqiang , Wang Zhongyuan , Zhang Xudong , Wu Shu , Chen Hongbo , Mei Lin , Zheng Yi 
Published: December 2012

Abstract
Cervical cancer remains a major problem in women's health worldwide. In this research, a novel biodegradable d-α-tocopheryl polyethylene glycol 1000 succinate-b-poly(ε-caprolactone-ran- glycolide) (TPGS-b-(PCL-ran-PGA)) nanoparticle (NP) was developed as a co-delivery system of docetaxel and endostatin for the synergistic treatment of cervical cancer. Docetaxel-loaded TPGS-b-(PCL-ran-PGA) NPs were prepared and further modified by polyethyleneimine for coating plasmid pShuttle2-endostatin. All NPs were characterized in size, surface charge, morphology, and in vitro release of docetaxel and pDNA. The uptake of coumarin 6-loaded TPGS-b-(PCL-ran-PGA)/PEI-pDsRED by HeLa cells was observed via fluorescent microscopy and confocal laser scanning microscopy. Endostatin expression in HeLa cells transfected by TPGS-b-(PCL-ran-PGA)/PEI-pShuttle2-endostatin NPs was detected using Western blot analysis, and the cell viability of different NP-treated HeLa cells was determined by MTT assay. The HeLa cells from the tumor model, nude mice, were treated with various NPs including docetaxel-loaded-TPGS-b-(PCL-ran-PGA)/PEI-endostatin NPs, and their survival time, tumor volume and body weight were monitored during regimen process. The tumor tissue histopathology was analyzed using hematoxylin and eosin staining, and microvessel density in tumor tissue was evaluated immunohistochemically. The results showed that the TPGS-b-(PCL-ran-PGA)/PEI NPs can efficiently and simultaneously deliver both coumarin-6 and plasmids into HeLa cells, and the expression of endostatin was verified via Western blot analysis. Compared with control groups, the TPGS-b-(PCL-ran-PGA)/PEI-pShuttle2-endostatin NPs significantly decreased the cell viability of HeLa cells (p < 0.01), inhibited the growth of tumors, and even eradicated the tumors. The underlying mechanism is attributed to synergistic anti-tumor effects by the combined use of docetaxel, endostatin, and TPGS released from NPs. The TPGS-b-(PCL-ran-PGA) NPs could function as multifunctional carrier for chemotherapeutic drugs and genetic material delivery, and offer considerable potential as an ideal candidate for in vivo cancer therapy.
Capacity-maximization threshold design for wideband sensing with guaranteed minimum primary user rate
Authors: Jia Peng , Le-Ngoc Tho 
Published: December 2012

Abstract
We investigate the design of optimal threshold for energy detection of primary user (PU) beacon signals in spectrum sensing. The weighted sum of the PU and secondary user (SU) link rates is used as the objective function with constraint on the guaranteed minimum PU link rate. SU with wideband sensing capability selects the idle frequency-slot with the lowest sensed power to be the candidate to access. Numerical results show that the wideband sensing capability with the proposed optimal threshold and accessing strategy can help the SU to meet the stringent PU rate constraints while achieving much higher SU link rate and network capacity.
Uncommon mandibular osteomyelitis in a cat caused by Nocardia africana
Authors: de Farias Marconi Rodrigues , Werner Juliana , Ribeiro Márcio Garcia , Rodigheri Sabrina Marin , Cavalcante Carolina Zaghi , Chi Kung Darh , Condas Larissa Anuska Zeni , Gonoi Tohru , Matsuzama Tetsuhiro , Yazama Katsukiyo 
Published: December 2012

Abstract

Background
Nocardiosis is an unusual infection in companion animals characterized by suppurative to pyogranulomatous lesions, localized or disseminated. Cutaneous-subcutaneous, pulmonary and systemic signs are observed in feline nocardiosis. However, osteomyelitis is a rare clinical manifestation in cats. Nocardia cyriacigeorgica (formerly N. asteroides sensu stricto), Nocardia brasiliensis, Nocardia otitidiscaviarum, and Nocardia nova are the most common pathogenic species identified in cats, based on recent molecular classification (16S rRNA gene). The present report is, to our knowledge, the first case of mandibular osteomyelitis in a cat caused by Nocardia africana, diagnosed based upon a combination of methods, including molecular techniques.

Case presentation
A one-year-old non-neutered female cat, raised in a rural area, was admitted to the Companion Animal Hospital-PUCPR, São José dos Pinhais, State of Paraná, Brazil, with a history a progressive facial lesion, difficulty apprehending food, loss of appetite, apathy and emaciation. Clinical examination showed fever, submandibular lymphadenitis, and a painless, 8 cm diameter mass, which was irregularly-shaped, of firm consistency, and located in the region of the left mandible. The skin around the lesion was friable, with diffuse inflammation (cellulitis), multiple draining sinuses, and exudation of serosanguinous material containing whitish “sulfur” granules.
Diagnosis was based initially in clinical signs, microbiological culture, cytological, and histopathological findings, and radiographic images. Molecular sequencing of 16S rRNA of isolate allowed diagnosis of Nocardia africana. Despite supportive care and antimicrobial therapy based on in vitro susceptibility testing the animal died.

Conclusion
The present report describes a rare clinical case of feline osteomyelitis caused by Nocardia africana, diagnosed based upon a combination of clinical signs, microbiological culture, cytological and histopathological findings, radiographic images, and molecular methods. The use of modern molecular techniques constitutes a quick and reliable method for Nocardia species identification, and may contribute to identification to new species of Nocardia that are virulent in cats.
Thymidylate synthase gene (TYMS) polymorphisms in sporadic and hereditary breast cancer
Authors: Junior José da Silva Nogueira , Marson Fernando Augusto de Lima , Bertuzzo Carmen Sílvia 
Published: December 2012

Abstract

Background
Breast cancer (BC) is a genetic disorder characterized by growth and proliferation of breast cells in a disorderly. In Brazil, there are approximately 49.240 new cases of BC, every year. The BC etiology is still poorly understood. The BC can be sporadic (SBC) or hereditary (HBC). Recent studies have correlated gene polymorphisms with the BC, such as alterations in thymidylate synthase gene (TYMS), which are used to improve diagnosis and prevention of the disease. Polymorphisms in the TYMS gene 5’-UTR region, usually present reps double (2R) and/or triple (3R). Studies have shown that homozygous 3R/3R is overexpressed compared with 2R/2R genotype, and these polymorphic variations may contribute to individual susceptibility to the development of BC. In this context, the objective of this study was to evaluate the frequency of the TYMS 2R and 3R polymorphisms, comparing genotypic and allelic distribution with SBC and HBC patients.

Methods
In this study we included a total of 204 subjects, 70 with BC (33 with SBC, and 37 with HBC) and 134 healthy subjects (controls). The Polymerase Chain Reaction was the method used.

Results
Results demonstrated a high frequency of the 3R allele at BC, SBC, and HBC groups. The frequency of genotype 2R/3R was significantly higher in BC group. This work showed association between the 2R/3R variants (OR = 4.14, CI95% = 1.77-9.71) in the development of SBC, and 2R/2R (OR = 0.233, CI95% = 1.63-7.65) and 2R/3R (OR = 3.53, CI95% = 0.06-0.81) for developing HBC. To BC, there was association with the genotype 2R/3R (OR: 3.79, CI95% = 2.03-7.08).

Conclusion
Our results show relation to the development of BC in association with the analyzed polymorphisms.
DNA methylation at the Igf2/H19 imprinting control region is associated with cerebellum mass in outbred mice
Authors: Pidsley Ruth , Fernandes Cathy , Viana Joana , Paya-Cano Jose L , Liu Lin , Smith Rebecca G , Schalkwyk Leonard C , Mill Jonathan 
Published: December 2012

Abstract

Background

Insulin-like growth factor 2 (Igf2) is a paternally expressed imprinted gene regulating fetal growth, playing an integral role in the development of many tissues including the brain. The parent-of-origin specific expression of Igf2 is largely controlled by allele-specific DNA methylation at CTCF-binding sites in the imprinting control region (ICR), located immediately upstream of the neighboring H19 gene. Previously we reported evidence of a negative correlation between DNA methylation in this region and cerebellum weight in humans.

Results
We quantified cerebellar DNA methylation across all four CTCF binding sites spanning the murine Igf2/H19 ICR in an outbred population of Heterogeneous Stock (HS) mice (n = 48). We observe that DNA methylation at the second and third CTCF binding sites in the Igf2/H19 ICR shows a negative relationship with cerebellar mass, reflecting the association observed in human post-mortem cerebellum tissue.

Conclusions
Given the important role of the cerebellum in motor control and cognition, and the link between structural cerebellar abnormalities and neuropsychiatric phenotypes, the identification of epigenetic factors associated with cerebellum growth and development may provide important insights about the etiology of psychiatric disorders.
SU11652 Inhibits tyrosine kinase activity of FLT3 and growth of MV-4-11 cells
Authors: Guo Yao , Chen Yun , Xu Xuesong , Fu Xueqi , Zhao Zhizhuang Joe 
Published: December 2012

Abstract

Background
FLT3-ITD and FLT3-TKD mutations are frequently found in acute myeloid leukemia (AML). This makes tyrosine kinase FLT3 a highly attractive target for therapeutic drug development. However, effective drugs have not yet emerged. This study is intended to identify and to characterize new FLT3 inhibitors.

Methods
By using the protein substrate GST-FLT3S to analyze kinase activity of recombinant proteins carrying the catalytic domain of wild type and mutant forms of FLT3, we screened a chemical library containing 80 known protein kinase inhibitors. We identified SU11652 as a potent FLT3 inhibitor and further employed FLT3-ITD-positive MV- 4–11 cells to study its effects on cell growth, apoptosis, cell cycles, and cell signaling.

Results
SU11652 strongly inhibited the activity of wild type, D835Y, and D835H mutant forms of FLT3 with IC50 values of 1.5, 16, and 32 nM, respectively. It effectively blocked the growth of FLT3-ITD -positive MV-4-11 cells at nanomolar concentrations but exhibited much less effects on several other cells which do not carry mutations of FLT3. SU11652 inhibited growth of MV-4-11 cells by inducing apoptosis, causing cell cycle arrest, and blocking activation of the ERK, Akt, and STAT signaling pathways.

Conclusion
SU11652 is a potent FLT3 inhibitor which selectively targets FLT3-ITD-positive cells. It should serve as a good candidate for development of therapeutic drugs to treat AML.
Tombusvirus P19 RNA silencing suppressor (RSS) activity in mammalian cells correlates with charged amino acids that contribute to direct RNA-binding
Authors: Liu Xiang , Houzet Laurent , Jeang Kuan-Teh 
Published: December 2012

Abstract

Background
Tombusvirus P19 is a protein encoded by tomato bushy stunt virus and related tombusviruses. Earlier studies have demonstrated that P19 is an RNA silencing suppressor (RSS) in plant cells. However, it has not been systematically investigated how P19 suppresses RNA interference in various mammalian cell settings.

Results
We have studied the RSS effect of P19 in mammalian cells, HEK293T, HeLa, and mouse embryonic fibroblasts. We have individually mutated 18 positively charged residues in P19 and found that 6 of these charged residues in P19 reduce its ability to suppress RNA interference. In each case, the reduction of silencing of RNA interference correlated with the reduced ability by these P19 mutants to bind siRNAs (small interfering RNAs).

Conclusions
Our findings characterize a class of RNA-binding proteins that function as RSS moieties. We find a tight correlation between positively charged residues in P19 accounting for siRNA-binding and their RSS activity. Because P19’s activity is conserved in plant and animal cells, we conclude that its RSS function unlikely requires cell type-specific co-factors and likely arises from direct RNA-binding.
MD Simulation Study of Ras/Raf Dissociation and the Resonating Structure of Deactivated Ras
Authors: Ogata Koji , Shen Jun-Wei , Sugawa Satoshi , Nakamura Shinichiro 
Published: December 2012

Abstract
Molecular dynamics (MD) simulations were used to investigate the dissociation of the Ras/Raf complex. Three models of Ras/Raf complexes were used in this study: two active GTP-bound forms with either GTP or GDP and one inactive GDP-bound form. The dissociation process in these short time scale (10 ns) MD simulations was accelerated by adding random velocities to phosphate groups in the GTP or GDP molecule. Only the complex with the inactive GDP-bound form heated with the random velocities showed dissociation. The Raf in it was unable to recover its initial position during the MD simulations. In simulations with and without added random velocities the Raf in the other complexes moved back to its initial position. The Raf in complexes with the active GTP-bound form recovered its initial position even when the ligands in the complexes were heated. The details of these mechanisms are discussed here.
Nondisjunctional Segregations in Drosophila Female Meiosis I Are Preceded by Homolog Malorientation at Metaphase Arrest
Authors: Gillies Shane C , Lane Fiona M , Paik Wonbeom , Pyrtel Khateriaa , Wallace Nneka T , Gilliland William D 
Published: December 2012

Abstract
The model of Drosophila female meiosis I was recently revised by the discovery that chromosome congression precedes metaphase I arrest. Use of the prior framework to interpret data from meiotic mutants led to the conclusion that chromosome segregation errors (nondisjunction, NDJ) occurred when nonexchange chromosomes moved out on the spindle in a maloriented configuration and became trapped there at metaphase arrest. The discovery that congression returns nonexchange chromosomes to the metaphase plate invalidates this interpretation and raises the question of what events actually do lead to NDJ. To address this, we have assayed an allelic series of ald (mps1) meiotic mutants that complete congression at wild-type rates, but have widely varying NDJ rates in an otherwise isogenic background, as well as a nod mutant background that primarily undergoes loss of chromosome 4. Using genetic assays to measure NDJ rates, and FISH assays to measure chromosome malorientation rates in metaphase-arrested oocytes, shows that these two rates are highly correlated across ald mutants, suggesting that malorientation during congression commits these chromosomes to eventually nondisjoin. Likewise, the rate of chromosome loss observed in nod is similar to the rate at which these chromosomes fail to associate with the main chromosome mass. Together these results provide a proximal mechanism for how these meiotic mutants cause NDJ and chromosome loss and improve our understanding of how prometaphase chromosome congression relates to anaphase chromosome segregation.
Adoption of IFRS in Brazil and the Consequences to Accounting Education
Authors: Carvalho L. Nelson , Salotti Bruno M. 
Published: December 2012

ABSTRACT:
Brazil is a rare case of a complete adoption of IFRS, not only for consolidated financial statements, but also for the individual ones. Very few countries dared to converge their accounting standards toward IFRS in the company-only financial statements, probably afraid of the tax or dividends impact. Brazil made the ambitious move, changing the Company law and altering the income tax legislation in such a way that a safe path was built to bridge from the old BR GAAP standards to IFRS.
Prolonged Curing Time Reduces the Effects of Simulated Pulpal Pressure on the Bond Strength of One-step Self-etch Adhesives
Authors: Feitosa VP , Watson TF , Vitti RP , Bacchi A , Correr-Sobrinho L , Correr AB , Sinhoreti MAC , Sauro S 
Published: December 2012

SUMMARY
The aim of this study was to evaluate the effects of extended light-curing procedures on the microtensile bond strength (μTBS) of one-step self-etch adhesives (1-SEAs) submitted to simulated pulpal pressure. Coronal deep-dentin specimens were bonded using Clearfil S3 Bond (S3), Adper Easy Bond (EB), or G-Bond Plus (GB) following the manufacturers' recommendations and light-cured for 10 seconds or 40 seconds. The dentin-bonded specimens were stored in distilled water for 24 hours without pulpal pressure (control) or submitted to 20 cm H2O simulated pulpal pressure for 24 hours. The specimens were cut into matchsticks and subjected to μTBS testing. The data were statistically analyzed using the three-way analysis of variance and Tukey's tests (p&lt;0.05). Debonded sticks were investigated through scanning electron microscopy. EB obtained higher bond strengths than GB and S3. However, prolonged light activation (40 seconds) provided higher μTBS for all adhesives when submitted to pulpal pressure. Conversely, pulpal pressure caused a drop in μTBS in EB and S3 when light-cured for 10 seconds. A mixed failure mode was mainly attained for the control groups, whereas the specimens submitted to pulpal pressure failed in the adhesive mode. The μTBS of GB was not affected by pulpal pressure when light-cured for 10 seconds. Adhesive was the most prevalent failure mode, except when light-cured for 40 seconds, which showed predominantly cohesive failure. Extended curing times improved the resistance of 1-SEAs to simulated pulpal pressure.
Long-term neurologic outcome of hemilaminectomy and disk fenestration for treatment of dogs with thoracolumbar intervertebral disk herniation: 831 cases (2000–2007)
Authors: Aikawa Takeshi , Fujita Hiroshi , Kanazono Shinichi , Shibata Mitsuhiro , Yoshigae Yuki 
Published: December 2012

Abstract
Objective—To determine the proportion of dogs with thoracolumbar intervertebral disk herniation (IVDH) that successfully recovered following hemilaminectomy and fenestration, the time to ambulation (TTA) in affected dogs after surgery, and the frequency of urinary and fecal incontinence in recovered dogs and to document long-term complications.
Design—Retrospective case series.
Animals—831 dogs with thoracolumbar IVDH treated by hemilaminectomy and concomitant disk fenestration by the same surgeon.
Procedures—For all dogs, neurologic deficits before surgery had been assessed with a modified grading system. Dogs were reexamined after surgery over a period of 3 to 6 months, and follow-up evaluation was performed at > 12 months. The proportion of dogs that neurologically improved after surgery, TTA, and incidence of fecal or urinary incontinence in recovered dogs were compared among dogs with various grades of neurologic dysfunction before surgery.
Results—Of 831 dogs, 122 had unsuccessful outcomes and 709 had successful outcomes. Of 620 dogs with intact deep nociception before surgery, 606 (97.7%) were ambulatory after surgery. Despite maintaining the ability to walk, 7 dogs were judged to have an unsuccessful outcome because the severity of ataxia did not improve. Of 211 paraplegic dogs with loss of deep nociception, 110 (52.1%) dogs became ambulatory after surgery. Long-term complications included incontinence, permanent neurologic deterioration, and self-mutilation. Dogs with paraplegia before surgery had a higher frequency of urinary or fecal incontinence, compared with dogs that were ambulatory.
Conclusions and Clinical Relevance—Prognosis for dogs with thoracolumbar IVDH that retain deep nociception in at least 1 of the pelvic limbs or tail before surgery was good.