bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allopolyploidization events and immense paleogenome reshuffling underlying the diversification of plants and secondary metabolites in Oleaceae
Authors: Wang Jiaqi , Ding Yue , Li Yinfeng , Gao Xintong , Kong Xiangming , Long Feng , Feng Yishan , Zhang Yan , Li Yu , Yu Zijian , Lei Tianyu , Wang Li , Li Xiu‐Qing , Wang Jinpeng 
Published: July 2024

AbstractOleaceae, a eudicot family with great species diversity, has attracted much attention from botanists because it contains many plants with important economic, medicinal, and ornamental values. However, the history of polyploidization and ancestral genome reshuffling of Oleaceae remains unclear. Here, we clarified an Oleaceae‐common hexaploidization (OCH) event occurring at ~53–61 million years ago (Ma) common in all Oleaceae plants and an Oleaceae‐recent tetraploidization (ORT) event occurring at ~18–21 Ma shared by the lineages of Syringa, Olea, Osmanthus, and Fraxinus. We found that high‐frequency polyploidization events drove the frequency of gene loss in Oleaceae genomes and extended the size of regions containing adjacent gene loss, thereby promoting the degree of genome fragmentation. We revealed that biased fractionation between the OCH‐ and ORT‐produced subgenomes is likely attributed to the origin of allopolyploidization in the OCH and ORT events. Significantly, through paleochromosome rearrangement comparisons, we proposed a "two‐step" genome duplication model for OCH and determined the duplicated orders of OCH tripled genome. We reconstructed 11 protochromosomes of the most recent ancestral Oleaceae karyotype (AOK) and elucidated the trajectories of immense paleochromosome reorganization of Oleaceae species from ancestral eudicot karyotype. Notably, we tracked the diversification history of secondary metabolite synthesis genes in the Oleaceae and explored the effects of paleogenome evolution on specialized metabolite synthesis. Our findings provide new insights into the polyploidization and paleogenomic evolution of Oleaceae and have important scientific significance for understanding the genetic basis of species and secondary metabolic diversity in Oleaceae.
Oral cancer in India
Authors: Ray Cecily S. , Gupta Prakash C. 
Published: July 2024

AbstractObjectivesTo describe the epidemiological status of oral cancer (OC) in India along with updates on risk factors, advances and gaps in preventive measures, treatment, costs of care and monitoring.MethodsTwo national estimates of incidence and mortality were cited, one for 2016 by the Global Burden of Disease India Study and one based on projections for 2020 by GLOBOCAN. The National Cancer Registry Project provided local and regional incidence and trends using data from 2012–2016. Scientific literature, reports of the NCRP, the IARC and government press releases were also consulted.ResultsTobacco and areca nut habits are the major risk factors for OC in India. In the 1980s, intervention studies on oral screening, awareness generation and habit cessation in rural areas reduced tobacco use and oral leukoplakia. Lately, adoption of the tobacco control law and mass media communications have resulted in decreased tobacco use. However, the increasing popularity of highly carcinogenic tobacco products containing areca nut, with evasion of their bans, is increasing OC incidence.ConclusionDespite advances in care, there is growing incidence of OC, persisting poor awareness about the need to quit tobacco/areca nut/alcohol use and go for screening. Major efforts are needed to implement preventive activities.
Shining light on diurnal variation of non‐photochemical quenching: Impact of gradual light intensity patterns on short‐term NPQ over a day
Authors: Lazzarin Martina , Driever Steven , Wassenaar Maarten , Marcelis Leo F. M. , van Ieperen Wim 
Published: July 2024

AbstractMaximal sunlight intensity varies diurnally due to the earth's rotation. Whether this slow diurnal pattern influences the photoprotective capacity of plants throughout the day is unknown. We investigated diurnal variation in NPQ, along with NPQ capacity, induction, and relaxation kinetics after transitions to high light, in tomato plants grown under diurnal parabolic (DP) or constant (DC) light intensity regimes. DP light intensity peaked at midday (470 μmol m‐2 s‐1) while DC stayed constant at 300 μmol m‐2 s‐1 at a similar 12‐hour photoperiod and daily light integral. NPQs were higher in the morning and afternoon at lower light intensities in DP compared to DC, except shortly after dawn. NPQ capacity increased from midday to the end of the day, with higher values in DP than in DC. At high light ΦPSII did not vary throughout the day, while ΦNPQ varied consistently with NPQ capacity. Reduced ΦNO suggested less susceptibility to photodamage at the end of the day. NPQ induction was faster at midday than at the start of the day and in DC than in DP, with overshoot occurring in the morning and midday but not at the end of the day. NPQ relaxation was faster in DP than in DC. The xanthophyll de‐epoxidation state and reduced demand for photochemistry could not explain the observed diurnal variations in photoprotective capacity. In conclusion, this study showed diurnal variation in regulated photoprotective capacity at moderate growth light intensity, which was not explained by instantaneous light intensity or increasing photoinhibition over the day and was influenced by acclimation to constant light intensity.
Partisanship and public support for presidential norms
Authors: James Chandler 
Published: July 2024

AbstractA prominent scholarship contends that presidential norms are particularly vital to a well‐functioning democracy. However, scant empirical research exists on the topic. I investigate public attitudes toward a novel battery of what many scholars and journalists consider presidential norms and examine the extent to which partisan and policy concerns affect attitudes toward them. Original survey data show that Americans strongly support presidential norms. I argue that there are two salient dimensions of presidential norms—what I call structural norms and presentation norms. Structural norms promote and support constitutional government. Presentation norms concern expectations about when and how presidents should present themselves before the public. Partisans evaluate the two types of presidential norms differentially, with Republicans notably expressing lower support for structural norms. I then present experimental evidence that the public's support for presidential norms is malleable to partisan framing. These results suggest that the public supports presidential norms in the abstract, but compelling rhetoric can facilitate presidential norm violations.
Characteristics of unilateral and bilateral bovine twin foetuses
Authors: Agerholm Jørgen Steen , Buelund Lene Elisabeth , Graff Katrine , Sørensen Tenna Vanggaard , Secher Jan Bojsen‐Møller 
Published: July 2024

AbstractTwin pregnancy in cattle is undesirable for a number of reasons, including a higher abortion risk compared to pregnancies with a single foetus. Yet, the abortion risk is significantly influenced by the intrauterine location of the foetuses, that is, the abortion risk is several times higher if they are implanted in the same uterine horn (unilateral twin pregnancy) than if they are implanted with one foetus in each uterine horn (bilateral twin pregnancy). The reason for the higher abortion risk in unilateral twin pregnancies is unknown, but it may be related to malnutrition of the outermost foetus due to a limited placental capacity, as is the case for equine twin foetuses. A slaughterhouse study was performed and the foetuses of cattle pregnant with twins were measured. We identified 65 cases of twin pregnancies, of which 35 were unilateral twin pregnancies and 30 were bilateral twin pregnancies. There was no significant difference between the outermost and the more centrally located foetus in unilateral twin pregnancies in terms of body weight and length of the metacarpal diaphysis. Growth retardation of the outermost foetus could therefore not be confirmed as the cause of the higher abortion risk in unilateral bovine twin pregnancies. Four cases of pre‐slaughter foetal mortality were identified. In three of these cases, both twins were dead, of equal size and at a comparable level of degradation. In the fourth case, with approximately 40‐day‐old twin foetuses of equal size, only one of the foetuses showed signs of pre‐slaughter death.
Age‐related decline in GABAergic intracortical inhibition can be counteracted by long‐term learning of balance skills
Authors: Kuhn Yves‐Alain , Egger Sven , Bugnon Matteo , Lehmann Nico , Taubert Marco , Taube Wolfgang 
Published: July 2024

AbstractAgeing induces a decline in GABAergic intracortical inhibition, which seems to be associated not only with decremental changes in well‐being, sleep quality, cognition and pain management but also with impaired motor control. So far, little is known regarding whether targeted interventions can prevent the decline of intracortical inhibition in the primary motor cortex in the elderly. Therefore, the present study investigated whether age‐related cortical dis‐inhibition could be reversed after 6 months of balance learning and whether improvements in postural control correlated with the extent of reversed dis‐inhibition. The results demonstrated that intracortical inhibition can be upregulated in elderly subjects after long‐term balance learning and revealed a correlation between changes in balance performance and intracortical inhibition. This is the first study to show physical activity‐related upregulation of GABAergic inhibition in a population with chronic dis‐inhibition and may therefore be seminal for many pathologies in which the equilibrium between inhibitory and excitatory neurotransmitters is disturbed.
imageKey points
Ageing induces a decline in GABAergic intracortical inhibition.
So far, little is known regarding whether targeted interventions can prevent the decline of intracortical inhibition in the primary motor cortex in the elderly.
After 6 months of balance learning, intracortical inhibition can be upregulated in elderly subjects.
The results of this study also revealed a correlation between changes in balance performance and intracortical inhibition.
This is the first study to show physical activity‐related upregulation of GABAergic inhibition in a population with chronic dis‐inhibition.
Cannabis use and acute postoperative pain outcomes in older adults: a propensity matched retrospective cohort study
Authors: Sajdeya Ruba , Rouhizadeh Masoud , Cook Robert L , Ison Ronald L , Bai Chen , Jugl Sebastian , Gao Hanzhi , Mardini Mamoun T , Dasa Osama , Zandbiglari Kimia , Adiba Farzana I , Winterstein Almut G , Price Catherine C , Pearson Thomas A , Seubert Christoph N , Tighe Patrick J 
Published: July 2024

IntroductionCannabis use is increasing among older adults, but its impact on postoperative pain outcomes remains unclear in this population. We examined the association between cannabis use and postoperative pain levels and opioid doses within 24 hours of surgery.MethodsWe conducted a propensity score-matched retrospective cohort study using electronic health records data of 22 476 older surgical patients with at least 24-hour hospital stays at University of Florida Health between 2018 and 2020. Of the original cohort, 2577 patients were eligible for propensity-score matching (1:3 cannabis user: non-user). Cannabis use status was determined via natural language processing of clinical notes within 60 days of surgery and structured data. The primary outcomes were average Defense and Veterans Pain Rating Scale (DVPRS) score and total oral morphine equivalents (OME) within 24 hours of surgery.Results504 patients were included (126 cannabis users and 378 non-users). The median (IQR) age was 69 (65–72) years; 295 (58.53%) were male, and 442 (87.70%) were non-Hispanic white. Baseline characteristics were well balanced. Cannabis users had significantly higher average DVPRS scores (median (IQR): 4.68 (2.71–5.96) vs 3.88 (2.33, 5.17); difference=0.80; 95% confidence limit (CL), 0.19 to 1.36; p=0.01) and total OME (median (IQR): 42.50 (15.00–60.00) mg vs 30.00 (7.50–60.00) mg; difference=12.5 mg; 95% CL, 3.80 mg to 21.20 mg; p=0.02) than non-users within 24 hours of surgery.DiscussionThis study showed that cannabis use in older adults was associated with increased postoperative pain levels and opioid doses.
Optimization of cervical cord synthetic T1-weighted MRI for enhancing clinical application in neurodegenerative spinal cord disorders
Authors: Schading-Sassenhausen Simon , Seif Maryam , Weiskopf Nikolaus , Freund Patrick 
Published: July 2024

Abstract
Synthetic MRI offers the advantage of reducing acquisition time and enhancing flexibility through the reconstruction of various contrast weightings from a single set of MRI scans. However, the use of synthetic T1-weighted (synT1-w) MRI can lead to potentially biased measurements of the cross-sectional area (CSA) in the spinal cord when compared to conventionally acquired T1-weighted MRI. This disparity can have implications for comparability and sensitivity of MRI in assessing disease progression or treatment effects in neurodegenerative spinal cord disorders. Thus, this study aimed at improving the accuracy (i.e., difference between synthetic and acquired MRI) of cervical cord CSA measurements (C1-C3 level) based on synT1-w MRI implementing a longitudinal data set acquired from 23 acute spinal cord injury (SCI) patients and 21 healthy controls over 2 years. Moreover, the validity of using synT1-w MRI for tracking cervical cord atrophy following SCI over 2 years was verified. SynT1-w images were reconstructed from quantitative maps of proton density, longitudinal, and effective transverse relaxation rates derived from a multi-parameter mapping protocol. The results showed a minimal bias of -0.31 mm2 (-0.5%) in CSA measurements based on synT1-w compared to acquired MRI. Estimates of atrophy rates and average CSA were comparable between synthetic and acquired MRI. A sample size estimation for detecting treatment effects on CSA atrophy after 2 years following SCI revealed that the required sample size is reduced by 13.5% using synT1-w instead of acquired MRI. This study shows high accuracy of synT1-w MRI and demonstrates its applicability in clinical studies for optimizing long MRI protocols.
Machine Learning Approach for Spatiotemporal Multivariate Optimization of Environmental Monitoring Sensor Locations
Authors: Siddiquee Masudur R. , Meray Aurelien O. , Xu Zexuan , Gonzalez-Raymat Hansell , Danielson Thomas , Upadhyay Himanshu , Lagos Leonel E. , Eddy-Dilek Carol , Wainwright Haruko M. 
Published: July 2024

Abstract
Long-term environmental monitoring is critical for managing the soil and groundwater at contaminated sites. Recent improvements in state-of-the-art sensor technology, communication networks, and artificial intelligence have created opportunities to modernize this monitoring activity for automated, fast, robust, and predictive monitoring. In such modernization, it is required that sensor locations be optimized to capture the spatiotemporal dynamics of all monitoring variables as well as to make it cost-effective. The legacy monitoring datasets of the target area are important to perform this optimization. In this study, we have developed a machine-learning approach to optimize sensor locations for soil and groundwater monitoring based on ensemble supervised learning and majority voting. For spatial optimization, Gaussian process regression (GPR) is used for spatial interpolation, while the majority voting is applied to accommodate the multivariate temporal dimension. Results show that the algorithms significantly outperform the random selection of the sensor locations for predictive spatiotemporal interpolation. While the method has been applied to a four-dimensional dataset (with two-dimensional space, time, and multiple contaminants), we anticipate that it can be generalizable to higher-dimensional datasets for environmental monitoring sensor location optimization.
Taxonomic and phenotypic analysis of bifidobacteria isolated from IBD patients as potential probiotic strains
Authors: Bosselaar Sabine , Dhelin Lucile , Dautel Ellena , Titecat Marie , Duthoy Stéphanie , Stelmaszczyk Marie , Delory Nathan , De Sousa Violante Madeleine , Machuron François , Ait-Abderrahim Hassina , Desreumaux Pierre , Foligné Benoit , Monnet Céline 
Published: July 2024

Abstract
Background
Inflammatory Bowel Diseases (IBD) are a major public health issue with unclear aetiology. Changes in the composition and functionality of the intestinal microbiota are associated with these pathologies, including the depletion of strict anaerobes such as Feacalibacterium prausnitzii. Less evidence is observed for depletion in other anaerobes, among which bifidobacteria. This study characterized the taxonomic and functional diversity of bifidobacteria isolated from the human intestinal microbiota in active and non-active IBD patients by a culturomics approach and evaluated if these bifidobacteria might be used as probiotics for gut health.

Results
A total of 341 bifidobacteria were isolated from the intestinal microbiota of IBD patients (52 Crohn’s disease and 26 ulcerative colitis patients), with a high proportion of Bifidobacterium dentium strains (28% of isolated bifidobacteria). In ulcerative colitis, the major species identified was B. dentium (39% of isolated bifidobacteria), in active and non-active ulcerative colitis. In Crohn’s disease, B. adolescentis was the major species isolated from non-active patients (40%), while similar amounts of B. dentium and B. adolescentis were found in active Crohn’s disease patients. The relative abundance of B. dentium was increased with age, both in Crohn’s disease and ulcerative colitis and active and non-active IBD patients. Antibacterial capacities of bifidobacteria isolated from non-active ulcerative colitis against Escherichia coli LF82 and Salmonella enterica ATCC 14028 were observed more often compared to strains isolated from active ulcerative colitis. Finally, B. longum were retained as strains with the highest probiotic potential as they were the major strains presenting exopolysaccharide synthesis, antibacterial activity, and anti-inflammatory capacities. Antimicrobial activity and EPS synthesis were further correlated to the presence of antimicrobial and EPS gene clusters by in silico analysis.

Conclusions
Different bifidobacterial taxonomic profiles were identified in the microbiota of IBD patients. The most abundant species were B. dentium, mainly associated to the microbiota of ulcerative colitis patients and B. adolescentis, in the intestinal microbiota of Crohn’s disease patients. Additionally, the relative abundance of B. dentium significantly increased with age. Furthermore, this study evidenced that bifidobacteria with probiotic potential (antipathogenic activity, exopolysaccharide production and anti-inflammatory activity), especially B. longum strains, can be isolated from the intestinal microbiota of both active and non-active Crohn’s disease and ulcerative colitis patients.
Optimizing placebo and minimizing nocebo effects through communication: e-learning and virtual reality training development
Authors: Westendorp Janine , van Vliet Liesbeth M. , Meeuwis Stefanie H. , olde Hartman Tim C. , Sanders Ariëtte R. J. , Jutten Eric , Dirven Monique , Peerdeman Kaya J. , Evers Andrea W. M. 
Published: July 2024

Abstract
Background
The effects of many treatments in healthcare are determined by factors other than the treatment itself. Patients’ expectations and the relationship with their healthcare provider can significantly affect treatment outcomes and thereby play a major role in eliciting placebo and nocebo effects. We aim to develop and evaluate an innovative communication training, consisting of an e-learning and virtual reality (VR) training, for healthcare providers across all disciplines, to optimize placebo and minimize nocebo effects through healthcare provider-patient communication. The current paper describes the development, mid-term evaluation, optimization, and final evaluation of the communication training, conducted in The Netherlands.

Methods
The development of both the e-learning and the VR training consisted of four phases: 1) content and technical development, 2) mid-term evaluation by healthcare providers and placebo/communication researchers, 3) optimization of the training, and 4) final evaluation by healthcare providers. To ensure the success, applicability, authenticity, and user-friendliness of the communication training, there was ongoing structural collaboration with healthcare providers as future end users, experts in the field of placebo/communication research, and educational experts in all phases.

Results
Placebo/communication researchers and healthcare providers evaluated the e-learning positively (overall 7.9 on 0–10 scale) and the content was perceived as useful, accessible, and interesting. The VR training was assessed with an overall 6.9 (0–10 scale) and was evaluated as user-friendly and a safe method for practicing communication skills. Although there were some concerns regarding the authenticity of the VR training (i.e. to what extent the virtual patient reacts like a real patient), placebo and communication researchers, as well as healthcare providers, recognized the significant potential of the VR training for the future.

Conclusions
We have developed an innovative and user-friendly communication training, consisting of an e-learning and VR training (2D and 3D), that can be used to teach healthcare providers how to optimize placebo effects and minimize nocebo effects through healthcare provider-patient communication. Future studies can work on improved authenticity, translate the training into other languages and cultures, expand with additional VR cases, and measure the expected effects on providers communication skills and subsequently patient outcomes.