bluuebunny/crossref_metadata_2025_split huggingface dataset:
Limited hydrolysis of β‐casein by cell wall proteinase and its effect on hydrolysates's conformational and structural properties
Authors: Ren Xiaofen , Pan Daodong , Wu Zhen , Zeng Xiaoqun , Sun Yangying , Cao Jinxuan , Guo Yuxing 
Published: November 2014

SummaryOptimisation of enzymatic hydrolysis of β‐casein with cell envelope proteinase (CEP) from Lactobacillus acidophilus JQ‐1 to produce the angiotensin‐I‐converting enzyme (ACE) inhibitory peptides using response surface methodology (RSM). Under optimal conditions (enzyme‐to‐substrate ([E]/[S]) ratio (w/w) of 0.132 and pH of 8.00 at 38.8 °C), the ACE inhibitory activity of hydrolysates was 72.06% and the total peptides was 11.75 mg mL−1. Scanning electron microscopy (SEM) micrographs indicated that the tightness of the β‐casein surface structure was gradually weakened and small holes appeared after enzymatic treatment, while Fourier transform infrared spectroscopy (FTIR) spectra indicated remarkable changes in the chemical composition and macromolecular conformation of β‐casein after enzymatic hydrolysis. Differential scanning calorimetry (DSC) analysis indicated that the corresponding hydrolysates had higher thermal stability. The enzymatic hydrolysis also led to an increase in the free sulfhydryl content of β‐casein hydrolysates compared with raw β‐casein, which led to the increase in the antioxidant activity of β‐casein hydrolysates.
High resolution U‐Pb ages of Ca‐phosphates in Apollo 14 breccias: Implications for the age of the Imbrium impact
Authors: Merle R. E. , Nemchin A. A. , Grange M. L. , Whitehouse M. J. , Pidgeon R. T. 
Published: November 2014

AbstractPrevious age estimates of the Imbrium impact range from 3770 to 3920 Ma, with the latter being the most commonly accepted age of this basin‐forming event. The occurrence of Ca‐phosphates in Apollo 14 breccias, interpreted to represent ejecta formed by this impact, provides a new opportunity to date the Imbrium event as well as refining the impact history of the Moon. We present new precise U‐Pb analyses of Ca‐phosphates from impact breccia sample 14311 that are concordant and give a reliable weighted average age of 3938 ± 4 Ma (2σ). Comparison with previously published U‐Pb data on phosphate from Apollo 14 samples indicate that all ages are statistically similar and suggest phosphates could have been formed by the same impact at 3934 Ma ± 3 Ma (2σ). However, this age is older than the 3770 to 3920 Ma range determined for other samples and also interpreted as formed during the Imbrium impact. This suggests that several impacts occurred during a 20–30 Ma period around 3900 Ma and formed breccias sampled by the Apollo missions.
The Motion of Emotion: Idiodynamic Case Studies of Learners' Foreign Language Anxiety
Authors: Gregersen Tammy , Macintyre Peter D. , Meza Mario D. 
Published: November 2014

ABSTRACTLanguage learning is an emotionally and psychologically dynamic process that is influenced by a myriad of ever‐changing variables and emotional “vibes” that produce moment‐by‐moment fluctuations in learners' adaptation. This individual‐level study triangulates physiological, idiodynamic, interview, and self‐report survey data of three high and three low anxiety language learners to examine their language anxiety, its triggers, and the interpretations of rapidly changing affective reactions over a short period of time. Participants were videorecorded giving a presentation, while wearing heart monitors, in their Spanish as a Foreign Language class. Using the idiodynamic method, participants self‐rated their moment‐by‐moment anxiety 42 times over three and a half minutes and later explained their reactions in an interview. The strong relationship observed among the various converging data sources demonstrates the strength of considering language learners on an individual level using triangulated quantitative and qualitative approaches. The study generated pedagogical implications for dealing with both positive and negative emotions, facilitating the reinterpretation of physiological cues, planning “escape routes” that allow participants to remain active in communication exchanges, and invoking the positive power of preparation, planning, and rehearsal.
Longitudinal Association Between Physical Activity and Body Fat During Adolescence: A Systematic Review
Authors: Ramires Virgílio Viana , Dumith Samuel Carvalho , Gonçalves Helen 
Published: November 2014

Background:Physical activity (PA) practice has been inversely associated to body fat (BF) and recommended as a way to reduce and prevent obesity. The objective of this study was to conduct a systematic review on the association of PA and BF in adolescence.Methods:The review includes 18 longitudinal studies found in the PubMed database, comprising papers published from January 1990 to July 2014. Studies assessing BF only through body mass index were excluded.Results:Among the outcomes analyzed, waist circumference, skinfolds, and absolute and relative fat mass measurement were identified. Questionnaires were the more predominant way to evaluate PA. Most studies showed that PA promotes a protective effect against a higher BF gain.Conclusion:It was concluded that PA has a protective effect against BF with differences between the genders and according to the BF marker or measurement assessed; higher intensity PA leads to a greater effect against BF gain in both genders; and the maintenance or increase of PA level on BF observed through analysis of change in PA level yielded more consistent findings in the relation between PA and BF.
First Experience of a Multicenter External Quality Assessment of Molecular 16S rRNA Gene Detection in Bone and Joint Infections
Authors: Plouzeau Chloé , Bémer Pascale , Valentin Anne Sophie , Héry-Arnaud Geneviève , Tandé Didier , Jolivet-Gougeon Anne , Vincent Pascal , Kempf Marie , Lemarié Carole , Guinard Jérôme , Bret Laurent , Cognée Anne Sophie , Gibaud Sophie , Burucoa Christophe , Corvec Stéphane 
Published: November 2014

ABSTRACT
The objective of this study was to assess the performance of seven French laboratories for 16S rRNA gene detection by real-time PCR in the diagnosis of bone and joint infection (BJI) to validate a large multicenter study. External quality control (QC) was required owing to the differences in extraction procedures and the molecular equipment used in the different laboratories. Three proficiency sets were organized, including four bacterial DNA extracts and four bead mill-pretreated osteoarticular specimens. Extraction volumes, 16S rRNA gene primers, and sequencing interpretation rules were standardized. In order to assess each laboratory's ability to achieve the best results, scores were assigned, and each QC series was classified as optimal, acceptable, or to be improved. A total of 168 QCs were sent, and 160 responses were analyzed. The expected results were obtained for 93.8%, with the same proportion for extracts (75/80) and clinical specimens (75/80). For the specimens, there was no significant difference between manual and automated extraction. This QC demonstrated the ability to achieve good and homogeneous results using the same 16S rRNA gene PCR with different equipment and validates the possibility of high-quality multicenter studies using molecular diagnosis for BJI.
Development and Evaluation of a Dipstick Diagnostic Test for Neisseria meningitidis Serogroup X
Authors: Agnememel Alain , Traincard François , Dartevelle Sylvie , Mulard Laurence , Mahamane Ali Elhaji , Oukem-Boyer Odile Ouwe Missi , Denizon Mélanie , Kacou-N′douba Adèle , Dosso Mireille , Gake Bouba , Lombart Jean-Pierre , Taha Muhamed-Kheir 
Published: November 2014

ABSTRACT

The emergence of
Neisseria meningitidis
serogroup X (NmX) in the African meningitis belt has urged the development of diagnostic tools and vaccines for this serogroup, especially following the introduction of a conjugate vaccine against
N. meningitidis
serogroup A (NmA). We have developed and evaluated a new rapid diagnostic test (RDT) for detecting the capsular polysaccharide (cps) antigen of this emerging serogroup. Whole inactivated NmX bacteria were used to immunize rabbits. Following purification by affinity chromatography, the cpsX-specific IgG antibodies were utilized to develop an NmX-specific immunochromatography dipstick RDT. The test was validated against purified cpsX and meningococcal strains of different serogroups. Its performance was evaluated against that of PCR on a collection of 369 cerebrospinal fluid (CSF) samples obtained from patients living in countries within the meningitis belt (Cameroon, Côte d'Ivoire, and Niger) or in France. The RDT was highly specific for NmX strains. Cutoffs of 10
5
CFU/ml and 1 ng/ml were observed for the reference NmX strain and purified cpsX, respectively. Sensitivity and specificity were 100% and 94%, respectively. A high agreement between PCR and RDT (Kappa coefficient, 0.98) was observed. The RDT gave a high positive likelihood ratio and a low negative likelihood (0.07), indicating almost 100% probability of declaring disease or not when the test is positive or negative, respectively. This unique NmX-specific test could be added to the available set of RDT for the detection of meningococcal meningitis in Africa as a major tool to reinforce epidemiological surveillance after the introduction of the NmA conjugate vaccine.
Nuclear Import of Adenovirus DNA Involves Direct Interaction of Hexon with an N-Terminal Domain of the Nucleoporin Nup214
Authors: Cassany Aurélia , Ragues Jessica , Guan Tinglu , Bégu Dominique , Wodrich Harald , Kann Michael , Nemerow Glen R. , Gerace Larry 
Published: November 2014

ABSTRACT

In this study, we characterized the molecular basis for binding of adenovirus (AdV) to the cytoplasmic face of the nuclear pore complex (NPC), a key step during delivery of the viral genome into the nucleus. We used RNA interference (RNAi) to deplete cells of either Nup214 or Nup358, the two major Phe-Gly (FG) repeat nucleoporins localized on the cytoplasmic side of the NPC, and evaluated the impact on hexon binding and AdV infection. The accumulation of purified hexon trimers or partially disassembled AdV at the nuclear envelope (NE) was observed in digitonin-permeabilized cells in the absence of cytosolic factors. Both
in vitro
hexon binding and
in vivo
nuclear import of the AdV genome were strongly reduced in Nup214-depleted cells but still occurred in Nup358-depleted cells, suggesting that Nup214 is a major binding site of AdV during infection. The expression of an NPC-targeted N-terminal domain of Nup214 in Nup214-depleted cells restored the binding of hexon at the NE and the nuclear import of protein VII (pVII), indicating that this region is sufficient to allow AdV binding. We further narrowed the binding site to a 137-amino-acid segment in the N-terminal domain of Nup214. Together, our results have identified a specific region within the N terminus of Nup214 that acts as a direct NPC binding site for AdV.

IMPORTANCE
AdVs, which have the largest genome of nonenveloped DNA viruses, are being extensively explored for use in gene therapy, especially in alternative treatments for cancers that are refractory to traditional therapies. In this study, we characterized the molecular basis for binding of AdV to the cytoplasmic face of the NPC, a key step for delivery of the viral genome into the nucleus. Our data indicate that a 137-amino-acid region of the nucleoporin Nup214 is a binding site for the major AdV capsid protein, hexon, and that this interaction is required for viral DNA import. These findings provide additional insight on how AdV exploits the nuclear transport machinery for infection. The results could promote the development of new strategies for gene transfer and enhance understanding of the nuclear import of other viral DNA genomes, such as those of papillomavirus or hepatitis B virus that induce specific cancers.
Proteomic Analysis of Ubiquitin-Like Posttranslational Modifications Induced by the Adenovirus E4-ORF3 Protein
Authors: Sohn Sook-Young , Bridges Rebecca G. , Hearing Patrick 
Published: November 2014

ABSTRACT
Viruses interact with and regulate many host metabolic pathways in order to advance the viral life cycle and counteract intrinsic and extrinsic antiviral responses. The human adenovirus (Ad) early protein E4-ORF3 forms a unique scaffold throughout the nuclei of infected cells and inhibits multiple antiviral defenses, including a DNA damage response (DDR) and an interferon response. We previously reported that the Ad5 E4-ORF3 protein induces sumoylation of Mre11 and Nbs1, which are essential for the DDR, and their relocalization into E4-ORF3-induced nuclear inclusions is required for this modification to occur. In this study, we sought to analyze a global change in ubiquitin-like (Ubl) modifications, with particular focus on SUMO3, by the Ad5 E4-ORF3 protein and to identify new substrates with these modifications. By a comparative proteome-wide approach utilizing immunoprecipitation/mass spectrometry, we found that Ubl modifications of 166 statistically significant lysine sites in 51 proteins are affected by E4-ORF3, and the proteome of modifications spans a diverse range of cellular functions. Ubl modifications of 92% of these identified sites were increased by E4-ORF3. We further analyzed SUMO3 conjugation of several identified proteins. Our findings demonstrated a role for the Ad5 E4-ORF3 protein as a regulator of Ubl modifications and revealed new SUMO3 substrates induced by E4-ORF3.

IMPORTANCE
The adenovirus E4-ORF3 protein induces dynamic structural changes in the nuclei of infected cells and counteracts host antiviral responses. One of the mechanisms that accounts for this process is the relocalization and sequestration of cellular proteins into an E4-ORF3 nuclear scaffold, but little is known about how this small viral protein affects diverse cellular responses. In this study, we analyzed for the first time the global pattern of ubiquitin-like (Ubl) modifications, with particular focus on SUMO3, altered by E4-ORF3 expression. The results suggest a role for the Ad5 E4-ORF3 protein as a regulator of Ubl modifications and reveal new SUMO3 substrates targeted by E4-ORF3. Our findings propose Ubl modifications as a new mechanism by which E4-ORF3 may modulate cellular protein functions in addition to subnuclear relocalization.
Clinical user experiences of observation and response charts: focus group findings of using a new format chart incorporating a track and trigger system
Authors: Elliott Doug , Allen Emily , Perry Lin , Fry Margaret , Duffield Christine , Gallagher Robyn , Iedema Rick , McKinley Sharon , Roche Michael 
Published: November 2014

BackgroundOptimising clinical responses to deteriorating patients is an international indicator of acute healthcare quality. Observation charts incorporating track and trigger systems are an initiative to improve early identification and response to clinical deterioration. A suite of track and trigger ‘Observation and Response Charts’ were designed in Australia and initially tested in simulated environments. This paper reports initial clinical user experiences and views following implementation of these charts in adult general medical-surgical wards.MethodsAcross eight trial sites, 44 focus groups were conducted with 218 clinical ward staff, mostly nurses, who received training and had used the charts in routine clinical practice for the preceding 2–6 weeks. Transcripts of audio recordings were analysed for emergent themes using an inductive approach.FindingsIn this exploration of initial user experiences, key emergent themes were: tensions between vital sign ‘ranges versus precision’ to support decision making; using a standardised ‘generalist chart in a range of specialist practice’ areas; issues of ‘clinical credibility’, ‘professional autonomy’ and ‘influences of doctors’ when communicating abnormal signs; and ‘permission and autonomy’ when escalating care according to the protocol. Across themes, participants presented a range of positive, negative or mixed views. Benefits were identified despite charts not always being used up to their optimal design function. Participants reported tensions between chart objectives and clinical practices, revealing mismatches between design characteristics and human staff experiences. Overall, an initial view of ‘increased activity/uncertain benefit’ was uncovered.ConclusionsFindings particularly reinforced the significant influences of organisational work-based cultures, disciplinary boundaries and interdisciplinary communication on implementation of this new practice chart. Optimal use of all chart design characteristics will be possible when these broader cultural issues are addressed.
Metastases in Immune-Mediated Dormancy: A New Opportunity for Targeting Cancer
Authors: Romero Irene , Garrido Federico , Garcia-Lora Angel M. 
Published: November 2014

AbstractThe aim of any anticancer treatment is to avoid, control, or eliminate disseminated tumor cells. Clinical and experimental evidence has revealed that metastases can remain in a latency state, that is, metastasis dormancy. Three mechanisms are thought to be involved in cancer dormancy: cellular dormancy, angiogenic dormancy, and immune-mediated dormancy. Here, we review the mechanisms and cells involved in immune-mediated cancer dormancy and discuss current and future immunotherapeutic strategies. Recent results indicate that the immune system can restrain disseminated cancer cells, promoting their permanent dormancy. CD8+ T lymphocytes play a relevant role in maintaining immune equilibrium with metastatic dormant cells, and MHC class I surface expression on tumor cells may also be involved. Natural killer (NK) cells have an activator function that triggers a cytotoxic T lymphocyte (CTL) response. Furthermore, immune dormancy promotes cancer cell growth arrest and angiogenic control. Immunotherapeutic interventions in metastatic dormancy may help to control or eradicate cancer disease. Treatments that activate or increase the CTL immune response or reverse cancer cell–induced CTL immunosuppression might be useful to restrain or destroy metastatic cells. These objectives may be achieved by recovering or increasing MHC class I surface expression on cancer cells or even by activating NK cells. Immune-mediated metastasis dormancy provides an opportunity for targeting cancer in novel immune treatments. Cancer Res; 74(23); 6750–7. ©2014 AACR.
Bursaphelenchus tiliae sp. n. (Nematoda: Parasitaphelenchidae), a nematode associate of the bark beetle Ernoporus tiliae (Panz.) (Coleoptera: Curculionidae, Scolytinae), in small-leaved lime, Tilia cordata Mill.
Authors: Tomalak Marek , Malewski Tadeusz 
Published: November 2014

Bursaphelenchus tiliae sp. n. is described from the bark of dead branches of the small-leaved lime, Tilia cordata, infested with the lime bark beetle, Ernoporus tiliae. The nematode adults and propagative juveniles were present in larval galleries, while dispersal dauer juveniles colonised the Malpighian tubules of adult beetles. Bursaphelenchus tiliae sp. n. is characterised by the body length of 664 (568-817) μm in female and 622 (515-754) μm in male, moderately slender body (a = 41.0 (34.7-47.9) and 43.1 (37.8-51.9) in female and male, respectively), and spicules 20.0 (18.1-21.8) μm long. The extended anterior vulval lip in female, lateral fields with four incisures, long post-uterine sac, and number (7) and arrangement of male caudal papillae, may indicate that B. tiliae sp. n. is closely related to the xylophilus group. It differs from this group by smaller, arcuate spicules with relatively short rostrum, narrow capitulum (5.2-6.9 μm), and indistinct condylus in an almost continuous line with dorsal lamina. Distal third of spicule dorsal contour is usually almost straight. The spicules have a distinct, disk-like cucullus at the terminus. The status of the new species is confirmed by the unique molecular profile of the ITS region (ITS-RFLP) and its close relation to the xylophilus group by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The presence of B. tiliae sp. n. dauer juveniles in Malpighian tubules of their vector bark beetle, E. tiliae, caused limited pathogenicity expressed as a local expansion of the tubule basement membrane and partial degradation of the cellular epithelium.
Qualitative Evaluation of Fiducial Markers for Radiotherapy Imaging
Authors: Chan Maria F. , Cohen Gil’ad N. , Deasy Joseph O. 
Published: November 2014

Purpose: To evaluate visibility, artifacts, and distortions of various commercial markers in magnetic resonance imaging (MRI), computer tomography (CT), and ultrasound imaging used for radiotherapy planning and treatment guidance. Methods: We compare 2 solid gold markers, 4 gold coils, and 1 polymer marker from 3 vendors. Imaging modalities used were 3-T and 1.5-T GE MRIs, Siemens Sequoia 512 Ultrasound, Phillips Big Bore CT, Varian Trilogy linear accelerator (cone-beam CT [CBCT], on-board imager kilovoltage [OBI-kV], electronic portal imaging device megavoltage [EPID-MV]), and Medtronic O-ARM CBCT. Markers were imaged in a 30 × 30 × 10 cm3 custom bolus phantom. In one experiment, Surgilube was used around the markers to reduce air gaps. Images were saved in Digital Imaging and Communications in Medicine (DICOM) format and analyzed using an in-house software. Profiles across the markers were used for objective comparison of the markers’ signals. The visibility and artifacts/distortions produced by each marker were assessed qualitatively and quantitatively. Results: All markers are visible in CT, CBCT, OBI-kV, and ultrasound. Gold markers below 0.75 mm in diameter are not visible in EPID-MV images. The larger the markers, the more CT and CBCT image artifacts there are, yet the degree of the artifact depends on scan parameters and the scanner itself. Visibility of gold coils of 0.75 mm diameter or larger is comparable across all imaging modalities studied. The polymer marker causes minimal artifacts in CT and CBCT but has poor visibility in EPID-MV. Gold coils of 0.5 mm exhibit poor visibility in MRI and EPID-MV due to their small size. Gold markers are more visible in 3-T T1 gradient-recalled echo than in 1.5-T T1 fast spin-echo, depending on the scan sequence. In this study, all markers are clearly visible on ultrasound. Conclusion: All gold markers are visible in CT, CBCT, kV, and ultrasound; however, only the large diameter markers are visible in MV. When MR and EPID-MV imagers are used, the selection of fiducial markers is not straightforward. For hybrid kV/MV image-guided radiotherapy imaging, larger diameter markers are suggested. If using kV imaging alone, smaller sized markers may be used in smaller sized patients in order to reduce artifacts. Only larger diameter gold markers are visible across all imaging modalities.
Submandibular approach and use of Ligasure® system in a complex carotid paraganglioma
Authors: Rojas Esquivel Diego José , Stefanov Kiuri Stefan , Fernández Heredero Alvaro , Riera De Cubas Luis , Carretero José Cebrian 
Published: November 2014

Carotid paragangliomas are rare tumors of slow growth, progressive, and highly vascularized; surgery is the chosen treatment, and most surgeons perform an approach with a longitudinal cervicotomy. Objective: We present a case of carotid paraganglioma Shamblin III with skull base extension. Methods: Submandibular approach and vascular sealing device of the Ligasure® system were used in the surgery. Results: We achieved complete resection using these adjunctive techniques. Conclusions: The use of these adjunctive techniques can be useful to manage large carotid body tumors.