bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bispecific and Trispecific Killer Cell Engagers Directly Activate Human NK Cells through CD16 Signaling and Induce Cytotoxicity and Cytokine Production
Authors: Gleason Michelle K. , Verneris Michael R. , Todhunter Deborah A. , Zhang Bin , McCullar Valarie , Zhou Sophia X. , Panoskaltsis-Mortari Angela , Weiner Louis M. , Vallera Daniel A. , Miller Jeffrey S. 
Published: October 2012

AbstractThis study evaluates the mechanism by which bispecific and trispecific killer cell engagers (BiKEs and TriKEs) act to trigger human natural killer (NK) cell effector function and investigates their ability to induce NK cell cytokine and chemokine production against human B-cell leukemia. We examined the ability of BiKEs and TriKEs to trigger NK cell activation through direct CD16 signaling, measuring intracellular Ca2+ mobilization, secretion of lytic granules, induction of target cell apoptosis, and production of cytokine and chemokines in response to the Raji cell line and primary leukemia targets. Resting NK cells triggered by the recombinant reagents led to intracellular Ca2+ mobilization through direct CD16 signaling. Coculture of reagent-treated resting NK cells with Raji targets resulted in significant increases in NK cell degranulation and target cell death. BiKEs and TriKEs effectively mediated NK cytotoxicity of Raji targets at high and low effector-to-target ratios and maintained functional stability after 24 and 48 hours of culture in human serum. NK cell production of IFN-γ, TNF-α, granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-8, macrophage inflammatory protein (MIP)-1α, and regulated and normal T cell expressed and secreted (RANTES) was differentially induced in the presence of recombinant reagents and Raji targets. Moreover, significant increases in NK cell degranulation and enhancement of IFN-γ production against primary acute lymphoblastic leukemia and chronic lymphocytic leukemia targets were induced with reagent treatment of resting NK cells. In conclusion, BiKEs and TriKEs directly trigger NK cell activation through CD16, significantly increasing NK cell cytolytic activity and cytokine production against tumor targets, showing their therapeutic potential for enhancing NK cell immunotherapies for leukemias and lymphomas. Mol Cancer Ther; 11(12); 2674–84. ©2012 AACR.
Rates and Predictors of 30-Day Readmission Among Commercially Insured and Medicaid-Enrolled Patients Hospitalized With Systolic Heart Failure
Authors: Allen Larry A. , Tomic Karen E. Smoyer , Smith David M. , Wilson Kathleen L. , Agodoa Irene 
Published: October 2012

Background—
Heart failure (HF) readmission rates are primarily derived from Medicare enrollees. Given increasing public scrutiny of HF readmissions, understanding the rate and predictors in populations covered by other payers is also important, particularly among patients with systolic dysfunction, for whom most HF-specific therapies are targeted.

Methods and Results—

MarketScan Commercial and Medicaid Administrative Claims Databases were used to identify all first hospitalizations with an International Classification of Diseases-9 discharge diagnosis code for HF (primary position) and systolic HF (any position) between January 1, 2005, and June 30, 2008. Among 4584 unique systolic HF index admissions (mean age 55 years), 30-day crude readmission rates were higher for Medicaid than commercially insured patients: all-cause 17.4% versus 11.8%; HF-related 6.7% versus 4.0%, respectively. In unadjusted analysis, higher comorbidity and prior healthcare utilization predicted readmission; age, sex, and plan type did not. After adjustment for case mix, the odds of all-cause and HF-related readmission were 32% and 68% higher, respectively, among Medicaid than commercially insured patients (
P
<0.02 for both). No significant differences in readmission rates were seen for managed care versus fee-for-service or capitated versus noncapitated plan types.

Conclusions—
Compared with commonly cited Medicare HF readmission rates of 20% to 25%, Medicaid patients with systolic HF had lower 30-day readmission rates, and commercially insured patients had even lower rates. Even after adjustment for case mix, Medicaid patients were more likely to be readmitted than commercially insured patients, suggesting that more attention should be focused on readmissions among socioeconomically disadvantaged populations.
Seasonal Changes in Solar Radiation and Relative Humidity in Europe in Response to Global Warming*
Authors: Ruosteenoja Kimmo , Räisänen Petri 
Published: October 2012

Abstract
Future seasonal changes in surface incident solar radiation and relative humidity (RH) over Europe and adjacent ocean areas were assessed based on phase 3 of the Coupled Model Intercomparison Project (CMIP3) model ensemble. Under the A1B scenario, by 2070–99, summertime solar radiation is projected to increase by 5%–10% in central and southern Europe. In winter, radiation decreases in most of northern and eastern Europe by 5%–15%. RH drops in summer in the southern European inland by 8%–12%, whereas in winter a small increase of 2%–3% is projected for northeastern Europe. In spring, the change is an intermediate between those in the extreme seasons, while in autumn the patterns resemble summer. Over the northern Atlantic Ocean, RH increases in all seasons by 1%–2%. The intermodel agreement on the sign of all these shifts is good, and the patterns recur in the responses to the A2 and B1 scenarios. Substantial changes are already simulated to occur before the midcentury, for example, in summer RH decreases by more than 5% in the inner Balkan Peninsula. Projected changes in these two variables agree well and are also mainly consistent with precipitation responses both in the multimodel mean and in individual models. According to all indicators, southern European summers become more arid, while winters, in the north particularly, become moister and darker. The increasing radiation and declining RH exacerbate summertime drought in southern Europe, whereas excessive humidity in the north may, for example, inflict moisture damages in constructions.
Does Knowing the Oceanic PDO Phase Help Predict the Atmospheric Anomalies in Subsequent Months?
Authors: Kumar Arun , Wang Hui , Wang Wanqiu , Xue Yan , Hu Zeng-Zhen 
Published: October 2012

Abstract
Based on analysis of a coupled model simulations with and without variability associated with the El Niño–Southern Oscillation (ENSO), it is demonstrated that knowing the current value of the ocean surface temperature–based index of the Pacific decadal oscillation (the OPDO index), and the corresponding atmospheric teleconnection pattern, does not add a predictive value for atmospheric anomalies in subsequent months. This is because although the OPDO index evolves on a slow time scale, it does not constrain the atmospheric variability in subsequent months, which retains its character of white noise stochastic variability and remains largely unpredictable. Further, the OPDO adds little to the atmospheric predictability originating from the tropical Pacific during ENSO years.
Evaluation of the Advanced Hurricane WRF Data Assimilation System for the 2009 Atlantic Hurricane Season
Authors: Cavallo Steven M. , Torn Ryan D. , Snyder Chris , Davis Christopher , Wang Wei , Done James 
Published: October 2012

Abstract
Real-time analyses and forecasts using an ensemble Kalman filter (EnKF) and the Advanced Hurricane Weather Research and Forecasting Model (AHW) are evaluated from the 2009 North Atlantic hurricane season. This data assimilation system involved cycling observations that included conventional in situ data, tropical cyclone (TC) position, and minimum SLP and synoptic dropsondes each 6 h using a 96-member ensemble on a 36-km domain for three months. Similar to past studies, observation assimilation systematically reduces the TC position and minimum SLP errors, except for strong TCs, which are characterized by large biases due to grid resolution. At 48 different initialization times, an AHW forecast on 12-, 4-, and 1.33-km grids is produced with initial conditions drawn from a single analysis member. Whereas TC track analyses and forecasts exhibit a pronounced northward bias, intensity forecast errors are similar to (lower than) the NWS Hurricane Weather Research Model (HWRF) and GFDL forecasts for forecast lead times ≤60 h (&gt;60 h), with the largest track errors associated with the weakest systems, such as Tropical Storm (TS) Erika. Several shortcomings of the data assimilation system are addressed through postseason sensitivity tests, including using the maximum 800-hPa circulation to identify the TC position during assimilation and turning off the quality control for the TC minimum SLP observation when the initial intensity is far too weak. In addition, the improved forecast of TS Erika relative to HWRF is shown to be related to having initial conditions that are more representative of a sheared TC and not using a cumulus parameterization.
The Bow and Arrow Mesoscale Convective Structure
Authors: Keene Kelly M. , Schumacher Russ S. 
Published: October 2012

Abstract
The accurate prediction of warm-season convective systems and the heavy rainfall and severe weather associated with them remains a challenge for numerical weather prediction models. This study looks at a circumstance in which quasi-stationary convection forms perpendicular to, and above the cold-pool behind strong bow echoes. The authors refer to this phenomenon as a “bow and arrow” because on radar imagery the two convective lines resemble an archer’s bow and arrow. The “arrow” can produce heavy rainfall and severe weather, extending over hundreds of kilometers. These events are challenging to forecast because they require an accurate forecast of earlier convection and the effects of that convection on the environment. In this study, basic characteristics of 14 events are documented, and observations of 4 events are presented to identify common environmental conditions prior to the development of the back-building convection. Simulations of three cases using the Weather Research and Forecasting Model (WRF) are analyzed in an attempt to understand the mechanisms responsible for initiating and maintaining the convective line. In each case, strong southwesterly flow (inducing warm air advection and gradual isentropic lifting), in addition to directional and speed convergence into the convective arrow appear to contribute to initiation of convection. The linear orientation of the arrow may be associated with a combination of increased wind speeds and horizontal shear in the arrow region. When these ingredients are combined with thermodynamic instability, there appears to be a greater possibility of formation and maintenance of a convective arrow behind a bow echo.
Cultural diversity in physical diseases among patients with mental illnesses
Authors: Larsen Jens I , Andersen Ulla A , Becker Thomas , Bickel Graziella G , Bork Bernhard , Cordes Joachim , Frasch Karel , Jacobsen Bent A , Jensen Signe O Wallenstein , Kilian Reinhold , Lauber Christoph , Mogensen Birthe , Nielsen Jørgen A , Rössler Wulf , Tsuchiya Kenji J , Uwakwe Richard , Munk-Jørgensen Povl 
Published: October 2012

Objective: People with psychiatric diseases have a severely increased risk for physical morbidity and premature death from physical diseases. The aims of the study were to investigate the occurrence of cardiovascular diseases (CVD), diabetes (DM) and obesity in schizophrenia and depression in three different geographical areas – Asia (Japan), Africa (Nigeria) and Western Europe (Switzerland, Germany and Denmark) – and to search for possible transcultural differences in these correlations, which would also reflect the differences between low-income areas in Africa (Nigeria) and high-income areas in Europe and Japan. Method: Patients with International Classification of Diseases (ICD-10) F2 diseases (schizophrenia spectrum disorders) and F3 diseases (affective disorders) admitted to one Nigerian, one Japanese, two Swiss, two German and six Danish centres during 1 year were included. Physical diseases in accordance with ICD-10 were also registered. Psychiatric and physical comorbidity were calculated and standardized rate ratio incidences of background populations were our primary measures. Results: Incidence rate ratios were increased for both CVD, DM and overweight in both F2 and F3 in all cultures (Western Europe, Nigeria and Japan) within the same ranges (however, the Japanese results should be interpreted conservatively owing to the limited sample size). Overweight among the mentally ill were marked in Nigeria. A parallelism of the incidence of overweight, CVD and diabetes with the occurrence in background populations was seen and was most marked in overweight. Conclusions: Overweight, CVD and DM were increased in schizophrenia spectrum disorders and affective disorders in all three cultures investigated (Western Europe, Nigeria and Japan). Lifestyle diseases were also seen in Nigeria and Japan. The results from this study indicate that cultural background might be seen as an important factor in dealing with lifestyle diseases among people with a severe mental illness, as it is in the general population.
Arthroscopic Agreement Among Surgeons on Anterior Cruciate Ligament Tunnel Placement
Authors: McConkey Mark O. , Amendola Annunziato , Ramme Austin J. , Dunn Warren R. , Flanigan David C. , Britton Carla L. ,   MOON Knee Group, Wolf Brian R. , Spindler Kurt P. , Carey James L. , Cox Charles L. , Kaeding Christopher C. , Wright Rick W. , Matava Matthew J. , Brophy Robert H. , Smith Matthew V. , McCarty Eric C. , Vidal Armando F. , Wolcott Michelle , Marx Robert G. , Parker Richard D. , Andrish Jack F. , Jones Morgan H. 
Published: October 2012

Background: Little is known about surgeon agreement and accuracy using arthroscopic evaluation of anterior cruciate ligament (ACL) tunnel positioning. Purpose: To investigate agreement on ACL tunnel position evaluated arthroscopically between operating surgeons and reviewing surgeons. We hypothesized that operating and evaluating surgeons would characterize tunnel positions significantly differently. Study Design: Controlled laboratory study. Methods: Twelve surgeons drilled ACL tunnels on 72 cadaveric knees using transtibial (TT), medial portal (MP), or 2-incision (TI) techniques and then completed a detailed assessment form on tunnel positioning. Then, 3 independent blinded surgeon reviewers each arthroscopically evaluated tunnel position and completed the assessment form. Statistical comparisons of tunnel position evaluation between operating and reviewing surgeons were completed. Three-dimensional (3D) computed tomography (CT) scans were performed and compared with arthroscopic assessments. Arthroscopic assessments were compared with CT tunnel location criteria. Results: Operating surgeons were significantly more likely to evaluate femoral tunnel position (92.6% vs 69.2%; P = .0054) and femoral back wall thickness as “ideal” compared with reviewing surgeons. Tunnels were judged ideal by reviewing surgeons more often when the TI technique was used compared with the MP and TT techniques. Operating surgeons were more likely to evaluate tibial tunnel position as ideal (95.5% vs 57.1%; P < .0001) and “acceptable” compared with reviewers. The ACL tunnels drilled using the TT technique were least likely to be judged as ideal on the tibia and the femur. Agreement among surgeons and observers was poor for all parameters (κ = −0.0053 to 0.2457). By 3D CT criteria, 88% of femoral tunnels and 78% of tibial tunnels were placed within applied criteria. Conclusion: Operating surgeons are more likely to judge their tunnels favorably than observers. However, independent surgeon reviewers appear to be more critical than results of 3D CT imaging measures. When subjectively evaluated arthroscopically, the TT technique yields more subjectively poorly positioned tunnels than the TI and MP techniques. Surgeons do not agree on the ideal placement for single-bundle ACL tunnels. Clinical Relevance: This study demonstrates that surgeons do not currently uniformly agree on ideal single-bundle tunnel placement and that the TT technique may yield more poorly placed tunnels.
A novel fibrous scaffold composed of electrospun porous poly(ɛ-caprolactone) fibers for bone tissue engineering
Authors: Nguyen Thi-Hiep , Bao Trinh Quang , Park Ihho , Lee Byong-Taek 
Published: October 2012

In this study, porous poly(ɛ-caprolactone) (PCL) fiber-based fibrous scaffolds are created using a suitable ratio of dimethyl chloride and acetone by using electrospinning. With the porous structure, it induced CaP particles to easily coat on the fibers after immersion in simulated body fluid solution. The morphology of the electrospun membranes was observed using scanning electron microscopic observation. The results showed that the CaP coated successfully, as examined by scanning electron microscopy, energy-dispersive spectroscopy, X-ray diffraction, transmission electron microscopic techniques and mass comparative analysis. The wettability of the coated fibrous scaffolds was tested using contact angle analysis. The in vitro cellular proliferation and cell interaction with fibrous scaffolds were investigated. In addition, the in vivo bone formation capacity of fibrous scaffolds including the non-porous (PCL-DCM), porous (PCL-DCM/Ace) and CaP coating on PCL/DCM-Ace for 2, 4, 8 and 12 h immersed in SBF solution were also investigated. By measuring the in vitro results, we verified that porous PCL fiber-based fibrous scaffold after 12 h of immersion in simulated body fluid (PCL-DCM/Ace-12) was excellent for cell interaction, growth and proliferation. The in vivo analyses showed that the PCL-DCM/Ace-12 fibrous scaffold enabled greater acceleration of bone formation than PCL/DCM and PCL/DCM-Ace fibrous scaffolds.
Hematologic and Clinical Parameters After Transcatheter Aortic Valve Implantation (TAVI) in Patients With Severe Aortic Stenosis
Authors: Gul Mehmet , Uyarel Huseyin , Akgul Ozgur , Uslu Nevzat , Yildirim Aydin , Eksik Abdurrahman , Aksu Hale Unal , Ozal Ender , Pusuroglu Hamdi , Erol Mustafa Kemal , Bakir Ihsan 
Published: October 2012

Background: Previous studies have demonstrated that platelet activation occurs in patients with aortic stenosis (AS). This study sought to evaluate the changes in hematologic and clinical parameters noted with the improvement in AS following transcatheter aortic valve implantation (TAVI) in patients with severe AS at high risk of surgery. Patients and Methods: The study included 33 patients who underwent TAVI. In addition to biochemical, clinical, and echocardiographic examinations, hematologic blood parameters were recorded before TAVI, at discharge, and at 1 and 4 months. Results: Mean platelet volume (MPV) showed a progressive decrease after TAVI. On echocardiography at 1 month, aortic valve area significantly increased, with significant decreases in peak and mean gradients. Progressive decreases were also noted in N-terminal proB-type natriuretic peptide levels. Conclusion: Our findings show that TAVI improves hemodynamic parameters of the valve with marked clinical and echocardiographic improvement, resulting in decreased platelet activation and MPV in patients with severe AS.
ACE2 gene polymorphisms and invasively measured central pulse pressure in cardiac patients indicated for coronarography
Authors: Vašků Anna , Bienertová-Vašků Julie , Pařenica Jiří , Goldbergová Monika Pávkova , Lipková Jolana , Zlámal Filip , Kala Petr. , Špinar Jindřich 
Published: October 2012

Background and aim: The objective of this research was to determine whether invasively measured central pulse pressure (PP) in patients indicated for coronarography is associated with two common polymorphisms in the ACE2 region (rs4646156 and rs4646174). Methods: A total of 307 patients were enrolled in the study. The genotyping of both SNPs from peripheral blood samples was carried out using 5′exonuclease (Taqman®) chemistry on the ABI Prism® 7000 system (Applied Biosystems, Foster City, CA, USA). Results: In both polymorphisms, the associations with central PP were found to be highly significant when all five possible genotypes in the population had been compared ( p = 0.0001). In men, there was a higher incidence of previous myocardial infarction in G0 genotype carriers of rs54646174 (OR ratio = 7; p = 0.005). The AA genotype of rs4646156 had a 7.81× higher risk of severe angina pectoris in women (OR = 7.81, p = 0.05). A significant difference in allelic frequency of ACE2rs4646174 was found between women with and without significant stenoses of the circumflex branch of the left coronary artery. Conclusion: More research into the role of ACE2 genetic variability in PP regulations is necessary for more detailed physiological and pathophysiological comprehension of PP regulation.
Notch is active in Langerhans cell histiocytosis and confers pathognomonic features on dendritic cells
Authors: Hutter Caroline , Kauer Max , Simonitsch-Klupp Ingrid , Jug Gunhild , Schwentner Raphaela , Leitner Judith , Bock Peter , Steinberger Peter , Bauer Wolfgang , Carlesso Nadia , Minkov Milen , Gadner Helmut , Stingl Georg , Kovar Heinrich , Kriehuber Ernst 
Published: October 2012

AbstractLangerhans cell histiocytosis (LCH) is an enigmatic disease defined by the accumulation of Langerhans cell-like dendritic cells (DCs). In the present study, we demonstrate that LCH cells exhibit a unique transcription profile that separates them not only from plasmacytoid and myeloid DCs, but also from epidermal Langerhans cells, indicating a distinct DC entity. Molecular analysis revealed that isolated and tissue-bound LCH cells selectively express the Notch ligand Jagged 2 (JAG2) and are the only DCs that express both Notch ligand and its receptor. We further show that JAG2 signaling induces key LCH-cell markers in monocyte-derived DCs, suggesting a functional role of Notch signaling in LCH ontogenesis. JAG2 also induced matrix-metalloproteinases 1 and 12, which are highly expressed in LCH and may account for tissue destruction in LCH lesions. This induction was selective for DCs and was not recapitulated in monocytes. The results of the present study suggest that JAG2-mediated Notch activation confers phenotypic and functional aspects of LCH to DCs; therefore, interference with Notch signaling may be an attractive strategy to combat this disease.