bluuebunny/crossref_metadata_2025_split huggingface dataset:
In Vivo Endothelial Cell Gene Silencing by siRNA‐LNPs Tuned with Lipoamino Bundle Chemical and Ligand Targeting
Authors: Yazdi Mina , Pöhmerer Jana , Hasanzadeh Kafshgari Morteza , Seidl Johanna , Grau Melina , Höhn Miriam , Vetter Victoria , Hoch Cosima C. , Wollenberg Barbara , Multhoff Gabriele , Bashiri Dezfouli Ali , Wagner Ernst 
Published: June 2024

AbstractAlthough small‐interfering RNAs (siRNAs) are specific silencers for numerous disease‐related genes, their clinical applications still require safe and effective means of delivery into target cells. Highly efficient lipid nanoparticles (LNPs) are developed for siRNA delivery, showcasing the advantages of novel pH‐responsive lipoamino xenopeptide (XP) carriers. These sequence‐defined XPs are assembled by branched lysine linkages between cationizable polar succinoyl tetraethylene pentamine (Stp) units and apolar lipoamino fatty acids (LAFs) at various ratios into bundle or U‐shape topologies. Formulation of siRNA‐LNPs using LAF4‐Stp1 XPs as ionizable compounds led to robust cellular uptake, high endosomal escape, and successful in vitro gene silencing activity at an extremely low (150 picogram) siRNA dose. Of significance is the functional in vivo endothelium tropism of siRNA‐LNPs with bundle LAF4‐Stp1 XP after intravenous injection into mice, demonstrated by superior knockdown of liver sinusoidal endothelial cell (LSEC)‐derived factor VIII (FVIII) and moderate silencing of hepatocyte‐derived FVII compared to DLin‐MC3‐DMA‐based LNPs. Optimizing lipid composition following click‐modification of siRNA‐LNPs with ligand c(RGDfK) efficiently silenced vascular endothelial growth factor receptor‐2 (VEGFR‐2) in tumor endothelial cells (TECs). The findings shed light on the role of ionizable XPs in the LNP in vivo cell‐type functional targeting, laying the groundwork for future therapeutic applications.
Aptamer and Thiol Co‐Regulated Color‐Shifting Fluorophores via Dynamic Through‐Bond/Space Conjugation for Constructing Ratiometric RNA Sensor
Authors: Ma Xuan , Zhang Yangzi , Zhu Longjiao , Wu Yifan , Li Jun , Huang Kunlun , Xu Wentao 
Published: June 2024

AbstractFluorophores with color‐shifting characteristics have attracted enormous research interest in the quantitative application of RNA sensors. It reports here a simple synthesis, luminescent properties, and co‐transcription ability of de‐conjugated triphenylmethane leucomalachite green (LMG). This novel clusteroluminescence fluorophore is rapidly synthesized from malachite green (MG) in reductive transcription system containing dithiothreitol, emitting fluorescence in the UV region through space conjugation. The co‐transcribed MG RNA aptamer (MGA) bound to the ligand, resulting in red fluorescence from the through‐bond conjugation. Given the equilibrated color‐shifting fluorophores, they are rationally employed in a 3WJ‐based rolling circle transcription switch, with the target‐aptamer acting as an activator to achieve steric allosterism. This one‐pot system allows the target to compete continuously for allosteric sites, and the activated transcription switches continue to amplify MGA forward, achieving accurate Aflatoxin 1 quantification at the picomolar level in 1 h. Due to the programmability of this RNA sensor, the design method of target‐competitive aptamers is standardized, making it universally applicable.
Fluorinated Co‐Solvents Enable Excellent Performances of Practical Cells Comprising LixSiO‐Graphite Composite Anodes and LiNi0.89Co0.05Mn0.05Al0.01O2 (NCMA) Cathodes
Authors: Vangapally Naresh , Lusztig David , Rathod Suman , Bano Amreen , Scalar Hadar , Akella Sri Harsha , Noked Malachi , Major Dan.T. , Halalay Ion C. , Sriramulu Suresh , Luski Shalom , Aurbach Doron 
Published: June 2024

AbstractLi‐ion batteries based on high specific capacity LixSiO‐Graphite anodes and LiNi0.89Co0.05 Mn0.05Al0.01O2 (NCMA) cathodes may have numerous practical applications owing to high energy density without a necessary compromise on safety. SiO, which is an attractive Li insertion anode material, offers more cycling stability than Si and a higher capacity than graphite. Therefore, a new trend has emerged for developing composite C‐Si anodes, possessing the excellent cyclability of graphite coupled with high capacity SiO. The composite structure described herein prevents the volume expansion of SiO and maintains the structural integrity during prolonged cycling. However, graphite electrodes suffer from exfoliation in propylene carbonate (PC) based electrolyte solutions, which avoids well known safety benefits related to a possible use of PC based electrolyte solutions in all kinds of Li batteries. Herein, it is reported that trifluoro propylene carbonate (TFPC) is compatible with graphite anodes. New electrolyte formulations are developed and tested containing fluorinated co‐solvents and compared the performance of several electrolyte solutions, including conventional alkyl carbonates‐based solutions in full Li‐ion cells, which included LixSiO‐Graphite anodes and LiNi0.89Co0.05Mn0.05Al0.01O2 (NCMA) cathodes. Cells with new electrolyte solutions developed herein demonstrated nearly twice capacity retention in prolonged cycling experiments compared to similar reference cells containing conventional electrolyte solutions.
Strong and Robust Core–Shell Ceramic Fibers Composed of Highly Compacted Nanoparticles for Multifunctional Electronic Skin
Authors: Hu Yunfeng , Cheng Zhi , Gao Jie , Liu Yongping , Yan Peng , Ding Qi , Fan Yuchi , Jiang Wan 
Published: June 2024

AbstractFunctional fibers composed of textiles are considered a promising platform for constructing electronic skin (e‐skin). However, developing robust electronic fibers with integrated multiple functions remains a formidable task especially when a complex service environment is concerned. In this work, a continuous and controllable strategy is demonstrated to prepare e‐skin‐oriented ceramic fibers via coaxial wet spinning followed by cold isostatic pressing. The resulting core–shell structured fiber with tightly compacted Al‐doped ZnO nanoparticles in the core and highly ordered aramid nanofibers in the shell exhibit excellent tensile strength (316 MPa) with ultra‐high elongation (33%). Benefiting from the susceptible contacts between conducting ceramic nanoparticles, the ceramic fiber shows both ultrahigh sensitivity (gauge factor = 2141) as a strain sensor and a broad working range up to 70 °C as a temperature sensor. Furthermore, the tunable core–shell structure of the fiber enables the optimization of impedance matching and attenuation of electromagnetic waves for the corresponding textile, resulting in a minimum reflection loss of −39.1 dB and an effective absorption bandwidth covering the whole X‐band. Therefore, the versatile core–shell ceramic fiber‐derived textile can serve as a stealth e‐skin for monitoring the motion and temperature of robots under harsh conditions.
The skilled worker premium and labor's share of income: Recent trends in U.S. manufacturing
Authors: Sedgley Norman , Elmslie Bruce 
Published: June 2024

AbstractA well‐established literature documents the role of outsourcing and technological change in explaining the growing skills premium in the U.S. economy from the 1970s through the late 1990s. In the 1980s labor's share of income relative to capital also began to decline. These two trends continue through the great recession. Separate literatures identify different reasons for the change in the skills premium and the decline in labor's share. This article considers the explanations for these two phenomena in a unified framework. The analysis is based on U.S. manufacturing data from 2002 through 2017, a period when there were significant shifts in the rate of growth in outsourcing and market concentration.
Faecal volatile organic compounds differ according to inflammatory bowel disease sub‐type, severity, and response to treatment in paediatric patients
Authors: Belnour Salma , Slater Rachael , Tharmaratnam Kukatharmini , Karl‐Heinz Auth Marcus , Muhammed Rafeeq , Spray Christine , Wang Duolao , Zeeshan Ijaz Umer , Probert Chris , Allen Stephen 
Published: June 2024

AbstractBackgroundFaecal volatile organic compounds (VOCs) differ with disease sub‐type and activity in adults with established inflammatory bowel disease (IBD) taking therapy.ObjectiveTo describe patterns of faecal VOCs in children newly presented with IBD according to disease sub‐type, severity, and response to treatment.MethodsChildren presenting with suspected IBD were recruited from three UK hospitals. Children in whom IBD was diagnosed were matched with a non‐IBD child for age, sex, and recruitment site. Faecal VOCs were characterised by gas chromatography–mass spectrometry at presentation and 3 months later in children with IBD.ResultsIn 132 case/control pairs, median (inter‐quartile range) age in IBD was 13.3 years (10.2–14.7) and 38.6% were female. Compared with controls, the mean abundance of 27/62 (43.6%) faecal VOCs was statistically significantly decreased in Crohn's disease (CD), ulcerative colitis (UC) or both especially amongst ketones/diketones, fatty acids, and alcohols (p < 0.05). Short‐chain, medium chain, and branched chain fatty acids were markedly reduced in severe colitis (p < 0.05). Despite clinical improvement in many children with IBD, the number and abundance of almost all VOCs did not increase following treatment, suggesting persistent dysbiosis. Oct‐1‐en‐3‐ol was increased in CD (p = 0.001) and UC (p = 0.012) compared with controls and decreased following treatment in UC (p = 0.01). In CD, propan‐1‐ol was significantly greater than controls (p < 0.001) and extensive colitis (p = 0.001) and fell with treatment (p = 0.05). Phenol was significantly greater in CD (p < 0.001) and fell with treatment in both CD (p = 0.02) and UC (p = 0.01).ConclusionCharacterisation of faecal VOCs in an inception cohort of children with IBD reveals patterns associated with diagnosis, disease activity, and extent. Further work should investigate the relationship between VOCs and the microbiome in IBD and their role in diagnosis and disease monitoring.
Investigation of prevalence and antimicrobial resistance of Salmonella in pet dogs and cats in Turkey
Authors: Yildiz Merve , Demirbilek Serpil Kahya 
Published: June 2024

AbstractBackgroundAlthough salmonellosis is considered to be a foodborne zoonotic disease, pets can play a significant role in the dissemination of antimicrobial‐resistant Salmonella organisms to humans because of close contact with their owners.ObjectivesTo determine the prevalence, risk factors, virulence factors, serotypes, and antimicrobial resistance profile of Salmonella in pet dogs and cats in Turkey and to assess the public health risk. Furthermore, to perform macroscopic comparison of lactic acid bacteria (LAB) in Salmonella‐positive and Salmonella‐negative animals.MethodsInternational Standards Organization (ISO) 6579‐1:2017 and Food and Drug Administration (FDA) methods were used to compare the effectiveness of culture methods in the identification of Salmonella in 348 rectal swabs. Positive isolates were serotyped using the slide agglutination method according to the White‐Kauffmann‐Le Minor scheme and the presence of virulence genes (invA and stn) were evaluated by polymerase chain reaction (PCR). Antimicrobial activity was tested by Kirby–Bauer disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines.ResultsSalmonella prevalence was 5.73% (9/157) in dogs and 0.0% (0/191) in cats. Eight (8/9) isolates were cultured with the ISO method and 5 (5/9) isolates were cultured with the FDA method. Macroscopic results revealed that Salmonella agents had no effect on LAB. Three different serotypes were detected and all isolates were positive for virulence genes. Antibiotic resistance profiling indicated that 11.1% of the isolates were MDR and the highest resistance was found for ciprofloxacin. MDR‐resistant S. Virchow and carbapenem‐resistant S. Enteritidis were detected from dog isolates. There was a significant difference between raw meat consumption and Salmonella carriage (p < 0.01).ConclusionsDogs could be potential carriers of Salmonella infection. The isolation of Salmonella in healthy dogs instead of dogs suffering from diarrhoea indicates that attention should be paid to asymptomatic carriage. The emergence of resistance among zoonotic Salmonella isolates poses a significant threat to public health.
Comparison of two different electrosurgical devices in reduction mammoplasty: Monopolar electrocautery versus plasmakinetic cautery
Authors: Koplay Tugba Gun 
Published: June 2024

AbstractBackgroundMany devices are used for dissection and hemostasis during reduction mammoplasty. While one of the most common methods is monopolar electrocautery, tissue damage due to thermal spread remains a controversial topic. New devices have been designed to minimize this effect. In this study, plasmakinetic cautery was hypothesized to reduce sensation loss, drainage, and wound‐healing problems in reduction mammoplasty because it is less harmful to the surrounding tissues.MethodsSixty‐eight patients were evaluated in a matched pair design, with random (blinded) assignment of 34 patients with conventional monopolar electrocautery (Group A) and 34 patients with plasmakinetic cautery (group B). Postoperative drainage volume, drain duration, nipple‐areolar complex (NAC) sensation, and complications (dehiscence, seroma, ischemia, and nipple circulatory problems) were compared by the researcher, who was blinded to the device used for the patient.ResultsThe groups were comparable in terms of age, body mass index (BMI), comorbidities, and medications (p > 0.05). The mean age of the patients were 38.50 ± 9.14 years in group A and 37.54 ± 8.17 in group B. The mean BMI was 25.19 ± 3.22 kg/m2 in group A and 25.65 ± 2.96 kg/m2 in group B. No differences were detected between the groups in terms of drain duration time, NAC sensation, or complications, but the drainage volume was statistically lower with plasmakinetic cautery (p < 0.05).ConclusionThe study findings indicate that the main advantage of plasmakinetic cautery in reduction mammoplasty was a decrease in drainage volume when compared with monopolar electrocautery.
Revealing the hidden RBP–RNA interactions with RNA modification enzyme‐based strategies
Authors: Jin Hua , Li Chong , Jia Yunxiao , Qi Yuxuan , Piao Weilan 
Published: June 2024

AbstractRNA‐binding proteins (RBPs) are powerful and versatile regulators in living creatures, playing fundamental roles in organismal development, metabolism, and various diseases by the regulation of gene expression at multiple levels. The requirements of deep research on RBP function have promoted the rapid development of RBP–RNA interplay detection methods. Recently, the detection method of fusing RNA modification enzymes (RME) with RBP of interest has become a hot topic. Here, we reviewed RNA modification enzymes in adenosine deaminases that act on RNA (ADAR), terminal nucleotidyl transferase (TENT), and activation‐induced cytosine deaminase/ApoB mRNA editing enzyme catalytic polypeptide‐like (AID/APOBEC) protein family, regarding the biological function, biochemical activity, and substrate specificity originated from enzyme selves, their domains and partner proteins. In addition, we discussed the RME activity screening system, and the RME mutations with engineered enzyme activity. Furthermore, we provided a systematic overview of the basic principles, advantages, disadvantages, and applications of the RME‐based and cross‐linking and immunopurification (CLIP)‐based RBP target profiling strategies, including targets of RNA‐binding proteins identified by editing (TRIBE), RNA tagging, surveying targets by APOBEC‐mediated profiling (STAMP), CLIP‐seq, and their derivative technology.This article is categorized under:
RNA Interactions with Proteins and Other Molecules > Protein‐RNA Recognition
RNA Processing > RNA Editing and Modification
The Peculiar Molecular [(IF5)6(HF2)4]4− Anion of the Compound K4[(IF5)6(HF2)4]
Authors: Koch Björn N. , Graubner Tim , Kraus Florian 
Published: June 2024

AbstractK4[(IF5)6(HF2)4] was obtained from the reaction of K[HF2] with liquid IF5. The compound was analyzed by single crystal and powder X‐ray diffraction, Raman spectroscopy, and quantum‐chemical calculations. It contains the largest molecular IF5/HF2− complex anion known so far. Surprisingly, the crystal structure of the A4B compound can be related to the Hg4Pt structure type.
Developing a Whole (Pre)school Approach to Sustainability: Insights from Global Citizenship and Early Childhood Education Across Nordic Countries
Authors: Borg Farhana , Samuelsson Ingrid Pramling , Gericke Niklas 
Published: June 2024

AbstractEarly childhood education aims to support children to develop cognitive, social, and motor skills, alongside values and ethics central to sustainability. From a Nordic perspective, this chapter reflects on how global citizenship education in preschool can illuminate possibilities and barriers to developing a whole school approach to sustainability. A review of Nordic policy and research identified four perspectives rooted in the global citizenship tradition that can support a whole school approach to sustainability:The greening of the whole preschool; The whole child’s learning; Wholeness in preschool teaching;andThematic approach as a perspective on a whole preschool.We propose thatefforts to implement a whole school approach to sustainability could be aligned with these perspectives. Nonetheless, Nordic preschools currently lack robust engagement with society—a perspective that requires further cultivation. To advance, empirical studies are needed to explore how a whole school approach to sustainability can be developed and implemented within preschool education.
Activating the Petals in the ‘WSA Flower Model’: Critical Factors for Sustaining Schools’ Pathways Towards a Whole-School Approach
Authors: Eikeland Ingrid , Sinnes Astrid 
Published: June 2024

AbstractIn this chapter, we describe four Norwegian upper secondary schools’ paths towards a whole school approach (WSA) to sustainability. Since 2017, these schools have been part of a longitudinal university–school partnership, together with a school owner and a teacher education. The aim of the partnership was to develop the learning institutions’ sustainability education. The data have been analysed through the lens of the ‘WSA flower model’ (Wals & Mathie, 2022). As a result, we found that the four schools took different paths towards a WSA (e.g. they were initiated by enthusiastic teachers or the leader group). Nonetheless, what the schools had in common was that more petals in the WSA flower model were activated as the partnership evolved. Furthermore, the activation of more petals was related to more focus on robust sustainability in the schools. Thus, we conclude that schools may follow different paths towards sustainability, yet the crucial factor seems to be that more than one petal in the WSA flower model needs to be activated to sustain a school’s journey towards a WSA. We also acknowledge the important role the overarching partnership played as a catalyst for both activating the petals and keeping them active.
Critical Events in the Systematic Work at an Organizational Level Towards a Whole School Approach to Sustainability in a Swedish Municipality
Authors: Manni Annika , Knekta Eva , Åberg Erika 
Published: June 2024

AbstractIn this chapter, we report from a 4-year study of the systematic work at an organizational level towards a whole school approach to sustainability. Aiming to extend the individual good practices on Education for Sustainable Development (ESD) to include all schools in the municipality, a process of school development began in 2019, and was studied for 4 years until 2022. Through a narrative approach, we describe the process and its critical events. Different dimensions of the school improvement model devised by Scherp and Scherp are also identified and analysed. The results show that a cyclic process, including not only different actions but also reflections, is important for improvement and, furthermore, that the holistic idea is an important dimension that should guide structures as well as teachers’ professional development towards more successful work with ESD in a whole-school organization.
Weaving Curriculum, Assessment, and Pedagogy: Global Citizenship Experience Lab School’s Whole-School Approach to Sustainability and Global Citizenship Education
Authors: Leite Stephanie 
Published: June 2024

AbstractThis chapter presents a case study of Global Citizenship Experience Lab School in Chicago, USA—a secondary school dedicated to integrating curriculum, assessment, and pedagogy to promote real-world experiential learning. The chapter analyzes the school’s use of scaffolding to introduce students and teachers to a project-based learning model and examines the school’s commitment to global citizenship as an entry point for a whole-school approach to sustainability. Theoretical contributions to the field of education for sustainable development recommend integrated, whole-school approaches to sustainability. However, schools transitioning to such a holistic model face many challenges due to the wider systems in which they are embedded. This case study exemplifies how approaching school redesign as a process of simultaneously rethinking curriculum, assessment, and pedagogy may instill a more relational way of thinking, which is essential if we are to transcend and transform the social and ecological crises of today.
Practicing Climate Action in a K-12 School Using a Whole Institution Approach
Authors: Hargis Kristen 
Published: June 2024

AbstractMost climate change education and research have focused on instilling individual scientific cognitive clarity instead of learning how to take climate actions together. This study utilized a practice lens to adjust focus away from an individual learner’s knowledge to the climate action practices collectively “carried,” (un)equally shared, and mutually shaped. Shove and colleagues’ conceptualization of practices was used to examine climate action practices at a Kindergarten to Grade 12 school in Canada using a whole institution approach to climate change education. A whole institution approach includes climate change education within and across each domain of Overall Governance, Teaching and Learning, Community Partnerships, and Facilities and Operations. Methods included a sensory walk, observations, interviews, focus groups, document collection, and photography. This chapter describes key themes identified within each of the whole institution domains with implications for a whole institution approach to CCE overall, as well as for each domain.
A Whole School Approach: A Synthesis of Interconnected Policy, Practice, and Research Conceptualisations
Authors: Mathie Rosalie Gwen 
Published: June 2024

AbstractThis explorative study examines the principles, problematics, and prospects of a Whole School Approach (WSA) by comparing 27 distinct, but inter-connected, WSA conceptualisations purposively selected from curriculum frameworks, policy documents, and academic literature. A WSA from an Education for Sustainable Development perspective forms the departure point of the study, conceptualisations from Education for Health and Wellbeing and Global Citizenship Education are then introduced. The chapter concludes that if a WSA is adopted to integrate individual education initiatives, such as reducing bullying, or a whole school climate action, it still runs the risk of further fostering siloed thinking and creating competition over what is prioritised. Instead, an inclusive understanding of the role of a WSA is essential, to ensure synergies between different but interconnected educational innovations are made: By framing a WSA as a reflexive thinking tool forgeneral quality educational change processes,grounded in holistic, systemic, and sustainable perspectives, a WSA has potential to support the integration of continual educational innovation processes and foster a proactive ‘culture of change and reflexivity’ within schools.