bluuebunny/crossref_metadata_2025_split huggingface dataset:
Superresolution STED microscopy reveals differential localization in primary cilia
Authors: Yang T. Tony , Hampilos Perry J. , Nathwani Bhavik , Miller Christine H. , Sutaria Nupur D. , Liao Jung‐Chi 
Published: November 2012

AbstractThe primary cilium is an organelle that serves as a signaling center of the cell and is involved in the cAMP, Wnt, and hedgehog signaling pathways. Adenylyl cyclase type III (ACIII) is enriched in primary cilia and acts as a marker that is involved in cAMP signaling, while also playing an important role in regulating ciliogenesis and sensory functions. Ciliary function relies on the transportation of molecules between the primary cilium and the cell, which is facilitated by intraflagellar transport (IFT). The detailed localization and interactions of these important proteins remain unclear due to the limited resolution of conventional microscopy. We conducted superresolution imaging of immunostained ACIII and IFT88 in human fibroblasts using stimulated emission depletion (STED) microscopy. Instead of a homogeneous distribution along a primary cilium, our STED images revealed that ACIII formed a periodic punctate pattern with a roughly equal spacing between groups of puncta. Superresolution imaging of IFT88, an important protein of the IFT complexes, demonstrated two novel distinct distribution patterns at the basal end: a triangle of three puncta with similar fluorescence intensities, and a Y‐shaped configuration of a bright punctum connected to two branches. We also performed STED imaging of IFT88 in mouse inner medullary collecting duct cells and mouse embryonic fibroblasts. The similar three‐puncta and Y‐shape patterns were observed in these cells, suggesting that these distribution patterns are common among primary cilia of different cell types. Our results demonstrate the ability of superresolution STED microscopy to reveal novel structural characteristics in primary cilia. © 2012 Wiley Periodicals, Inc
Resurrected Ceriodaphnia quadrangula highlight differences between pheno‐ and genotypic expressions
Authors: Reinikainen Marko , Åhlén Emma 
Published: November 2012

AbstractThe hatching of cladoceran ephippia from a 15‐cm long sediment core was investigated, and Ceriodaphnia quadrangula clones were isolated from different sediment layers. Bosmina microfossil data were also analyzed, and compared with the corresponding data from a Pb210 dated core, which allowed us to infer the age of the sediment layers. Using changes in Bosmina microfossil morphologies, we were, furthermore, able to infer the presence of different regimes of fish predation. C. quadrangula was found to hatch in layers with an inferred age of approximately a century. Newly hatched individuals had smaller eye‐size in sediment layers corresponding to high predation by young‐of‐the‐year perch. Newly hatched individuals also generally had a marked neck‐spine. In contrast, morphological characters of C. quadrangula clones reared in the laboratory over several generations showed no variation in relation to predation regime, indicating the absence of fixed genotype level changes. Furthermore, the laboratory grown clones only rarely produced a neck‐spine. The results suggest phenotypic variation in response to the regime under which ephippia were produced.
Is the change of winter wheat yield under warming caused by shortened reproductive period?
Authors: Hou Ruixing , Ouyang Zhu , Li Yunsheng , Wilson Glenn V. , Li Hanxia 
Published: November 2012

AbstractPrevious reports from laboratory‐controlled experiments and models considered that a shorter reproductive period could be the main reason for wheat yield reduction in the warmer world. However, this conclusion needs to be proved carefully by field‐scale experiments. In this study, a field‐scale continuous open‐warming experiment was conducted to quantify the adjustment of winter wheat growth and yield under conventional tillage (CT) and no‐till (NT) systems in the North China Plain (NCP). Canopy temperatures were warmed using infrared heaters between 1.0 and 1.6°C (daytime and nighttime, respectively) above the control. Wheat yields under CT were not significantly reduced over the two seasons (2010 and 2011), but yields under NT were 3.3% and 6.1% lower, respectively. The growing seasons for both CT and NT were shortened 6 days in 2010 and 11 days in 2011; however, the reproductive periods were maintained. The shortened days were due to a significantly shorter springtime re‐greening stage followed by minimal changes in other phenological stages (jointing, flag completed, heading, anthesis, and grain‐filling). The temporal advance by warming resulted in lower growing‐season mean air temperatures (MAT) for warmed plots than the control from 0.23 to 4.22°C for the same subsequent phenological stages. Warming increased the number of tillers m−2 and kernel weight, but tended to decrease the number of spikes m−2 in the two tillage systems. The heavier kernels offset the yield reduction from smaller number of spikes. Warming increased the wheat aboveground biomass from 10% to 20% suggesting the potential to sequester more CO2. This study suggests that winter wheat might adjust its growth (shortened vegetative period to maintain reproductive period) to partly compensate for the negative effects from global warming in this temperate irrigated cropland.
Changes in soil bacterial community triggered by drought‐induced gap succession preceded changes in soil C stocks and quality
Authors: Yuste Jorge Curiel , Barba Josep , Fernandez‐Gonzalez Antonio José , Fernandez‐Lopez Manuel , Mattana Stefania , Martinez‐Vilalta Jordi , Nolis Pau , Lloret Francisco 
Published: November 2012

AbstractThe aim of this study was to understand how drought‐induced tree mortality and subsequent secondary succession would affect soil bacterial taxonomic composition as well as soil organic matter (SOM) quantity and quality in a mixed Mediterranean forest where the Scots pine (Pinus sylvestris) population, affected by climatic drought‐induced die‐off, is being replaced by Holm‐oaks (HO; Quercus ilex). We apply a high throughput DNA pyrosequencing technique and 13C solid‐state Nuclear Magnetic Resonance (CP‐MAS 13C NMR) to soils within areas of influence (defined as an surface with 2‐m radius around the trunk) of different trees: healthy and affected (defoliated) pines, pines that died a decade ago and healthy HOs. Soil respiration was also measured in the same spots during a spring campaign using a static close‐chamber method (soda lime). A decade after death, and before aerial colonization by the more competitive HOs have even taken place, we could not find changes in soil C pools (quantity and/or quality) associated with tree mortality and secondary succession. Unlike C pools, bacterial diversity and community structure were strongly determined by tree mortality. Convergence between the most abundant taxa of soil bacterial communities under dead pines and colonizer trees (HOs) further suggests that physical gap colonization was occurring below‐ground before above‐ground colonization was taken place. Significantly higher soil respiration rates under dead trees, together with higher bacterial diversity and anomalously high representation of bacteria commonly associated with copiotrophic environments (r‐strategic bacteria) further gives indications of how drought‐induced tree mortality and secondary succession were influencing the structure of microbial communities and the metabolic activity of soils.
Exploring the genetics of nestling personality traits in a wild passerine bird: testing the phenotypic gambit
Authors: Brommer Jon E. , Kluen Edward 
Published: November 2012

AbstractWhen several personality traits covary, they form a behavioral syndrome. Understanding the evolutionary dynamics of a behavioral syndrome requires knowledge of its genetic underpinning. At present, our understanding of the genetic basis of behavioral syndromes is largely restricted to domestic and laboratory animals. Wild behavioral syndromes are mostly inferred on the basis of phenotypic correlations, and thus make the “phenotypic gambit” of assuming that these phenotypic correlations capture the underlying genetic correlations. On the basis of 3 years of reciprocal cross‐fostering of 2896 nestlings of 271 families within a pedigreed population, we show that the nestling personality traits handling aggression, breathing rate, and docility are heritable (h2 = 16–29%), and often have a pronounced “nest‐of‐rearing” variance component (10–15%), but a relatively small “nest‐of‐origin” variance component (0–7%). The three nestling personality traits form a behavioral syndrome on the phenotypic and genetic level. Overall, the phenotypic correlations provide a satisfactory description of the genetic ones, but significantly underestimate the magnitude of one of the pairwise genetic correlations, which mirrors the conclusion based on domestic and laboratory studies.
Rational Synthesis of a Family of Neutral Monomeric Heteroleptic Titanium Complexes Based on an Octahedral TiO4N2 Motif
Authors: Weekes David Michael , Baradel Nathalie , Kyritsakas Nathalie , Mobian Pierre , Henry Marc 
Published: November 2012

AbstractA rational approach leading to a novel family of monomeric heteroleptic titanium complexes formed with two biphenolate derivatives and six different bidentate nitrogenated chelates is proposed. Only one case of loss of structural predictability was observed in the case of a N,O‐coordinating ligand and led to a structure containing two (μ‐O)‐bridged titanium centres. Diffusion‐ordered NMR spectroscopy (DOSY) measurements have been systematically used to prove that the complexes characterised in the solid state remain identical in the liquid phase. All ligands have also been classified quantitatively according to their electron‐withdrawal capabilities. A quantitative thermodynamic analysis using the PACHA software showed that the entropically favoured displacement of two monodentate‐coordinated alcohol molecules needs to be assisted by solvation of the η2‐chelate product to overcome the enthalpically unfavourable replacement of two highly polar Ti–O bonds by less polar Ti–N bonds. The occurrence of dichloromethane molecules in the crystalline state and the existence of more than one crystallographically nonequivalent complex within the unit cell has been associated with a low dipolar moment of the complexes. Other complexes with large dipolar moments (μ > 2 D) seem to have enough dipolar energy in the solid state to remove all the solvating dichloromethane molecules.
A Molecular Precursor Approach to 3D Aligned CuInS2 Chalcopyrite Nanorod Arrays
Authors: Pashchanka Mikhail , Hoffmann Rudolf C. , Schneider Jörg J. 
Published: November 2012

AbstractPolycrystalline ternary CuInS2 (CIS) nanorods with stoichiometric composition have been synthesized by using a nanocasting approach employing track‐etched polycarbonate films as soft templates. The CuInS2 chalcopyrite phase was obtained by using molecular copper and indium oximato complexes and thiourea as single‐source precursors.
C,N‐Palladacycles Containing N‐Heterocyclic Carbene and Azido Ligands – Effective Catalysts for Suzuki–Miyaura Cross‐Coupling Reactions
Authors: Kim Yong‐Joo , Lee Jung‐Hyun , Kim Taejung , Ham Jungyeob , Zheng Zhen Nu , Lee Soon W. 
Published: November 2012

AbstractAzido‐palladacycles containing C,N‐donor and N‐heterocyclic carbene ligands, [(C,N‐L)Pd(N3)(NHC)] [NHC = IPr; 1,3‐bis(2,6‐diisopropylphenyl)imidazol‐2‐ylidene], were prepared from (i) IPr and dinuclear PdII azides, [Pd(μ‐N3)(C,N‐Ln)]2 [C,N‐L1H = N,N′‐dimethylbenzylamine; C,N‐L2H = 2‐(2′‐thienyl)pyridine; C,N‐L3H = azobenzene; C,N‐L4H = 2‐(p‐tolyl)pyridine], or from (ii) NaN3 and mononuclear PdII chloride, [Pd(Cl)(IPr)(C,N‐Ln)], in aqueous solution. The structures of two of these products were determined by X‐ray crystallography. The palladacycles showed high catalytic efficiency toward activated and nonactivated aryl bromides (at room temperature) as well as aryl chlorides (at 80 °C) in Suzuki–Miyaura cross‐coupling reactions. In addition, coupling reactions of aryl chlorides using potassium aryl trifluoroborates instead of phenyl boronic acid gave good‐to‐excellent product yields.
A Polyoxometalate‐Based Coordination Polymer with the Diamondoid Topology
Authors: Mandic Susanna , Healey Merinda R. , Gotthardt Jan M. , Alley Kerwyn G. , Gable Robert W. , Ritchie Chris , Boskovic Colette 
Published: November 2012

AbstractThe new coordination polymer [ϵ‐PMo12O37(OH)3{La(H2O)3}4(L)]·28H2O [H4L = tetrakis(4‐carboxyphenyl)silane] can be synthesized by combining equal quantities of two types of 4‐connecting building blocks: organic L4– ligands and inorganic polyoxometalate‐derived [ϵ‐PMo12O37(OH)3{La(H2O)4}4]4+ polyoxocations. The resulting coordination polymer exhibits the diamondoid topology, where both building blocks act as pseudotetrahedral nodes. The structural and spectroscopic characteristics of the parent polyoxocation are retained in the coordination polymer.
Synthesis of Cyclotriveratrylene–Phenylacetylene Derivatives and a Photophysical Investigation of Rigid Conjugated Cyclotriveratrylene Dendrimers
Authors: Han Xiao‐Na , Chen Jian‐Ming , Huang Zhi‐Tang , Zheng Qi‐Yu 
Published: November 2012

AbstractVarious functionalized host molecules and building blocks can be derived from the cone‐shaped cyclotriveratrylene (CTV). In this article, we report on the synthesis of CTV‐I3 derivatives 3a and 3b and their Sonogashira cross‐coupling with various terminal aryl‐alkynes, to synthesize CTV‐phenylacetylene derivatives in moderate to good yields. In this way, three star‐like conjugated dendrimers with rigid macrocyclic cores 4k–m, which showed good photophysical properties, were also synthesized. The band gaps of 4k–m are 3.49, 3.49 and 3.45 eV, and the fluorescence quantum yields are 0.64, 0.78, 0.48 (2‐aminopyridine as a standard and excited at 310 nm), respectively. Electrochemical studies revealed their HOMO and LUMO energy levels. Our research disclosed that these CTV dendrimers could potentially be used as host materials in OLEDs.
One‐Pot Catalyst‐Free Synthesis of β‐ and γ‐Hydroxy Sulfides Using Diaryliodonium Salts and Microwave Irradiation
Authors: Vaddula Buchi Reddy , Varma Rajender S. , Leazer John 
Published: November 2012

AbstractA simple and rapid one‐pot protocol is described for the preparation of α‐ and β‐hydroxy sulfides. The direct solvent‐free microwave irradiation of diaryliodonium salts, potassium thiocyanate, and ethylene glycol/β‐propylene glycol without any catalyst or base affords the final compounds in good yields (65–95 %) in around 10–25 min.
miR‐10b*, a master inhibitor of the cell cycle, is down‐regulated in human breast tumours
Authors: Biagioni Francesca , Bossel Ben‐Moshe Noa , Fontemaggi Giulia , Canu Valeria , Mori Federica , Antoniani Barbara , Di Benedetto Anna , Santoro Raffaela , Germoni Sabrina , De Angelis Fernanda , Cambria Anna , Avraham Roi , Grasso Giuseppe , Strano Sabrina , Muti Paola , Mottolese Marcella , Yarden Yosef , Domany Eytan , Blandino Giovanni 
Published: November 2012

AbstractDeregulated proliferation is a hallmark of cancer cells. Here, we show that microRNA‐10b* is a master regulator of breast cancer cell proliferation and is downregulated in tumoural samples versus matched peritumoural counterparts. Two canonical CpG islands (5 kb) upstream from the precursor sequence are hypermethylated in the analysed breast cancer tissues. Ectopic delivery of synthetic microRNA‐10b* in breast cancer cell lines or into xenograft mouse breast tumours inhibits cell proliferation and impairs tumour growth in vivo, respectively. We identified and validated in vitro and in vivo three novel target mRNAs of miR‐10b* (BUB1, PLK1 and CCNA2), which play a remarkable role in cell cycle regulation and whose high expression in breast cancer patients is associated with reduced disease‐free survival, relapse‐free survival and metastasis‐free survival when compared to patients with low expression. This also suggests that restoration of microRNA‐10b* expression might have therapeutic promise.
Personalised medicine: challenges in development and delivery
Authors: Montgomery David A , Heathfield Adam , Moledina Altaf 
Published: November 2012

AbstractOn 9 December 1969, in an open letter to President Nixon published in the Washington Post, Dr Sidney Farber, past president of the American Cancer Society was quoted as saying: ‘We are so close to a cure for cancer’.1 So what happened? Copyright © 2012 John Wiley & Sons, Ltd.
Drugs in development for the management of dyslipidaemia
Authors: Wierzbicki Anthony , Hardman Timothy , Viljoen Adie 
Published: November 2012

AbstractLipid‐lowering is established as proven intervention to reduce atherosclerosis. Statins form the basis of care but are not able to treat all aspects of dyslipidaemia. Many novel therapeutic compounds are being developed. These include additional therapeutics for low‐density lipo protein cholesterol (LDL‐C), eg antisense oligonucleotides or microsomal transfer protein inhibitors (MTPI); triglycerides, eg novel peroxisomal proliferator activating receptors (PPAR) agonists, MTPIs; HDL: delipidation strategies and cholesterol ester transfer protein inhibitors (CETPIs) and modulators of inflammation, eg phospholipase inhibitors. Copyright © 2012 John Wiley & Sons, Ltd.
Aflibercept – a novel agent for VEGF‐driven macular disease
Authors: Morphis George 
Published: November 2012

AbstractAflibercept is a novel vascular endothelial growth factor (VEGF) inhibitor that has recently been approved by the US Food and Drug Administration (FDA) for the treatment of wet age‐related macular degeneration (AMD). In this Drug profile Mr George Morphis presents the clinical data relating to its efficacy and adverse events, and discusses its potential place in therapy. Copyright © 2012 John Wiley & Sons, Ltd.
Diffuse reflectance spectroscopy in Barrett’s Esophagus: developing a large field‐of‐view screening method discriminating dysplasia from metaplasia
Authors: Douplik Alexandre , Zanati Simon , Saiko Guennadi , Streutker Catherine , Loshchenov Maxim , Adler Desmond , Cho Sarah , Chen Dean , Cirocco Maria , Marcon Norman , Fengler John , Wilson Brian C. 
Published: November 2012

AbstractWe evaluated diffuse reflectance spectroscopy implemented as a small field‐of‐view technique for discrimination of dysplasia from metaplasia in Barrett’s esophagus as an adjuvant to autofluorescence endoscopy. Using linear discriminant analysis on 2579 spectra measured in 54 patients identified an optimum a 4‐wavelength classifier (at 485, 513, 598 and 629 nm). Sensitivity and specificity for a test data set were 0.67 and 0.85, respectively. Spectroscopic results show that this technique could be implemented in wide‐field imaging mode to improve the accuracy of existing endoscopy techniques for finding early pre‐malignant lesions in Barrett’s esophagus. Results show that the discrimination occurs likely due to redistribution of blood content in the tissue sensed by the optical probing with the wavelength‐dependent sampling depth. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Characterisation of a label‐free biosensor based on long period grating
Authors: Chiavaioli Francesco , Trono Cosimo , Giannetti Ambra , Brenci Massimo , Baldini Francesco 
Published: November 2012

AbstractOptical fibre gratings, especially long period gratings, have been recently proposed as optical devices for biochemical sensing. A biochemical interaction along the grating portion induces a refractive index change and hence a change in the fiber transmission spectrum. This provides an alternative methodology with respect to other label‐free optical approaches, such as surface plasmon resonance, interferometric configurations and optical resonators. The fibre biofunctionalization has been carried out by means of a novel chemistry using Eudragit L100 copolymer as opposed to the commonly used silanization procedure. Antigen‐antibody interaction has been analysed by means of an IgG/anti‐IgG bioassay. The biosensor was fully characterised, monitoring the kinetics during the antibody immobilization and the antigen interaction and achieving the calibration curve of the assay. A comparison of the biosensor performance was made by using two different long period gratings with distinct periods. (© 2014 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Profiling pluripotent stem cells and organelles using synchrotron radiation infrared microspectroscopy
Authors: Sandt Christophe , Frederick Joni , Dumas Paul 
Published: November 2012

AbstractFTIR micro‐spectroscopy is a sensitive, non‐destructive and label‐free method offering diffraction‐limited resolution with high signal‐to‐noise ratios when combined with a synchrotron radiation source. The vibrational signature of individual cells was used to validate an alternative strategy for reprogramming induced pluripotent stem cells generated from amniocytes. The iPSC lines PB09 and PB10, were reprogrammed from the same amniocyte cell line using respectively the Oct54, Sox2, Lin28, and Nanog and the Oct4 and Sox2 transcription factor cocktail. We show that cells reprogrammed by the two different sets of transfection factors have similar spectral signatures after reprogramming, except for a small subpopulation of cells in one of the cell lines. Mapping HeLa cells at subcellular resolution, we show that the Golgi apparatus, the cytoplasm and the nucleus have a specific spectral signature. The CH3:CH2 ratio is the highest in the nucleus and the lowest in the Golgi apparatus/endoplasmic reticulum, in agreement with the membrane composition of these organelles. This is confirmed by specific staining of the organelles with fluorescent dyes. Subcellular differentiation of cell compartments is also demonstrated in living cells. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Quantitative analysis of dynamic behavior of osteoblasts during in vitro formation of micro‐mass cell cultures
Authors: Schäfer Susanne , Dekiff Markus , Plate Ulrich , Szuwart Thomas , Denz Cornelia , Dirksen Dieter 
Published: November 2012

AbstractImprovements in bone tissue engineering require an understanding of cellular and tissue level behavior of osteoblast‐like cells. Experiments indicate that in the absence of an anchoring material, intercellular adhesion may be based on signals that promote cell activity resulting in the formation of a spheroid cell‐matrix. The aim of the present study is to investigate the formation of scaffold‐free three‐dimensional micro‐mass cell spheroids in vitro, and to characterize quantitatively the cell movement. A new correlation based automated tracking method is evaluated in order to optimize the processing parameters and to identify statistical parameters that characterize the cell behavior. Results suggest that the temporal development of the mean distance of the cells to the center of gravity may be described by an exponential function, thus providing a characteristic time constant as a quantitative measure of cell dynamics. (© 2013 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
An Experimental and Theoretical Study on Influence of H‐bonding on the Synthesized Azo Derivatives Structures
Authors: Amiri Rahebeh , Haghdadi Mina , Golshani Ashkan , Amiri Maryam Mohamadian , Barikani Maryam 
Published: November 2012

AbstractWe have reported a simple and efficient reaction for the synthesis of some new functionalized azo structures which were prepared by electron deficient acetylenic compounds in the presence of triphenylphosphine. The characterization of the synthesized azo compounds has been determined by FTIR, UV‐Vis, 1H NMR, 13C NMR and Mass spectroscopic techniques. The influence of H‐bonding on the products has been shown by different experimental analysis. Also, the regioselectivity of the reaction, tautomerization equilibrium and the stability of products was investigated using DFT calculations at the B3LYP/6‐31G level of theory.