bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lithium and venlafaxine poisoning — a case report
Authors: Sinkovič Andreja , Vrbnjak Matej , Svensek Franci , Kirbis Simona 
Published: June 2013

Abstract
In treatment of manic-depressive conditions long-term lithium therapy may be combined with an effective and relatively safe antidepressant venlafaxine. Combined overdose may increase the risk of early toxicity of both drugs and of delayed lithium intoxication, responding to symptomatic and renal replacement therapy. We present a patient with combined lithium and venlafaxine self-poisoning with nothing but delayed signs of lithium intoxication with the emphasis on early and late treatment. 41-year old woman attempted suicide by large amount of lithium and venlafaxine. On admission she was asymptomatic, but with increased serum lithium over 5mmol/L. After gastric lavage, active charcoal and laxative administration she was receiving IV fluids. After a delay of 63 hours she deteriorated acutely by disorientation, confusion, fasciculation and tremor and was readmitted to Intensive care unit. In spite serum lithium decreased to 2mmol/L clinical signs were attributed to delayed lithium intoxication. After symptomatic and renal replacement therapy the patient’s condition improved after few days. We conclude that decontamination procedures are effective in particular for venlafaxine poisoning. If increased serum lithium levels are noted renal replacement therapy may be started even in asymptomatic patients as delayed lithium intoxication is most likely after few days.
Effect of exopolymeric substances on the kinetics of sorption and desorption of trivalent chromium in soil
Authors: Kantar Cetin , Demir Aydeniz , Koleli Nurcan 
Published: June 2013

AbstractLaboratory batch sorption-desorption and column experiments were performed to better understand the effects of microbial exopolymeric substances (EPS) on Cr(III) sorption/desorption rates in the soil-water system. The experiments were carried out in two different modes: one mode (sorption) in which Cr(III) and EPS were applied simultaneously, and the other (desorption) included the sequential application of Cr(III) and EPS to the soil-water system. The batch sorption and desorption experiments showed that, while chromium(III) desorption was significantly enhanced in the presence of EPS relative to non-EPS-containing systems, the desorption rates were much smaller than the sorption rates, and the fraction dissolved by EPS accounted for only a small portion of the total chromium initially sorbed onto soil minerals. Similarly, the column experiments suggested that, while the microbial EPS led to an increase in Cr dissolution relative to non-EPS-containing systems, only a small portion of the total chromium initially added to the soil was mobilised. The differences observed in Cr sorption and desorption rates can be explained through the very low solubility and strong interactions of chromium species with soil minerals as well as the mass transfer effects associated with low diffusion rates. The overall results suggest that, while microbial EPS may play an important role in microbial Cr(VI) treatment in sub-surface systems due to the formation of soluble Cr-EPS complexes, the extent and degree of Cr mobilisation are highly dependent on the type of initial Cr sorption.
Simultaneous determination of 32 antibiotics in aquaculture products using LC-MS/MS
Authors: Fedorova Ganna , Nebesky Vaclav , Randak Tomas , Grabic Roman 
Published: June 2013

AbstractAn analytical multiclass, multi-residue method for the determination of antibiotics in aquaculture products was developed and validated. A fast, cheap, and straightforward extraction procedure followed by liquid chromatography-tandem mass spectrometry analysis was proposed. This method covers 32 antibiotics of different classes, which are frequently used in aquaculture. Three different extraction procedures were compared, and the extraction with acetonitrile (0.1 vol. % formic acid) showed the best results. The selected extraction procedure was validated at four different fortification levels (10 μg kg−1, 25 μg kg−1, 50 μg kg−1, and 100 μg kg−1). Recoveries of the tested antibiotics ranged from 70 % to 120 %, with the relative standard deviation (RSD) of triplicates lower than 20 %. The limits of quantification (LOQ) ranged from 0.062 μg kg−1 to 4.6 μg kg−1, allowing for the analysis of trace levels of these antibiotics in aquaculture products. The method was applied to the analysis of selected antibiotics in fish and shrimp meat available in the Czech market.
Keratitis, Ichthyosis and Deafness (KID) Syndrome – a Case Report
Authors: Stepanović Milica , Paravina Mirjana , Janković Goran , Janjić Spasić Danica 
Published: June 2013

AbstractKeratitis, ichthyosis and deafness (KID) syndrome is a rare congenital ectodermal dysplasia characterized by ichthyosiform hyperkeratosis of the skin, neurosensory hearing loss and vascularizing keratitis. It is inherited as an autosomal dominant trait, now known to be due to mutations in the connexin gene.This paper presents a case of a 20-year-old male patient with erythrokeratodermia and mild scaling since birth. He presented mild hearing impairment at the age of two and often suffered from eye inflammations. On admission, his clinical picture was typical of KID syndrome with erythrokeratodermia, neurosensory deafness, vascularizing keratitis, alopecia, palmoplantar keratosis, and nail dystrophy. The patient also had a history of recurrent infections, especially bacterial and candidal infections of the skin, auditory canals and eyes. Despite extensive skin, ocular, ear and hair manifestations, his physical and psychomotor growth and development were normal.Adjuvant balneotherapy in Prolom Spa, along with emollient creams, significantly reduced cutaneous manifestations in our patient.
Focal thyroid incidental uptake detected by 18F-fluorodeoxyglucose positron emission tomography
Authors: Treglia G. , Bertagna F. , Sadeghi R. , Verburg F. A. , Ceriani L. , Giovanella L. 
Published: June 2013

SummaryAim: To perform a meta-analysis of published data on the prevalence and risk of malignancy of focal thyroid incidental uptake (FTIs) detected by Fluorine-18-Fluorodeoxyglucose (18F-FDG) positron emission tomography (PET) or PET/CT. Methods: A comprehensive literature search of studies published up to and including December 2012 was performed. Pooled prevalence and malignancy risk of FTIs were calculated, including a sub-analysis for the geographic areas of origin of the studies. Results: 34 studies including 215 057 patients were selected. Pooled prevalence of FTIs was 1.92% (95% confidence interval [95%CI]: 1.87–1.99%). Overall, 1522 FTIs underwent histopathology evaluation. Pooled risk of malignancy was 36.2% (95%CI: 33.8–38.6%), without significant differences among various geographic areas. Conclusions: FTIs are observed in about 2% of 18F-FDG-PET or PET/CT scans and carry a significant risk of malignancy. Therefore, further investigation is warranted whenever FTIs are detected by 18F-FDG-PET or PET/CT.
Sport Injuries in Elite Paralympic Swimmers With Visual Impairment
Authors: Silva Marilia Magno e , Bilzon James , Duarte Edison , Gorla Jose , Vital Roberto 
Published: June 2013

Context:Visually impaired athletes sustain overuse injuries in the lower limbs, but the pattern of injuries may vary according to the sport. The characteristics of sports injuries in visually impaired swimmers are unknown.Objective:To determine the characteristics and epidemiologic measures (prevalence, clinical incidence, and incidence rate) of sports injuries in visually impaired elite national swimmers and to assess differences among visual classes and between sexes.Design:Descriptive epidemiology study.Setting:International Paralympic competitions held between 2004 and 2008.Patients or Other Participants:Twenty-eight elite, visually impaired swimmers (19 males, 9 females) from the Brazilian Paralympic Team participated in this study. Twelve were visual class S11 (blind swimmers), 12 were S12 (low-vision swimmers), and 4 were S13 (low-vision swimmers).Intervention(s):A standardized report form was used to collect data during 5 competitions. This was endorsed by 2 Brazilian sports governing bodies.Main Outcome Measures:The characteristics, prevalence, clinical incidence, and incidence rate of injuries were calculated.Results:Eighteen athletes reported 41 sports injuries, with a prevalence of 64%, clinical incidence of 1.5 injuries per athlete, and an incidence rate of 0.3 injuries per athlete per competition. Overuse injuries (80%) were more frequent than traumatic injuries (20%). The clinical incidence and prevalence varied according to sex and visual class, but no statistical differences were observed (P &gt; .05). The highest proportion of injuries was in the trunk (46.34%), followed by the upper limbs (34.15%). The shoulders (29.27%) were most affected, followed by the thoracic (21.95%) and lumbar spine (17.07%). Spasm (36.59%) was the most frequent diagnosis, followed by tendinopathy (26.83%).Conclusions:Visually impaired swimmers had a relatively high proportion of overuse injuries, predominantly associated with muscle spasm in the spine and tendinopathy in the shoulders. No differences were apparent in injury prevalence and clinical incidence among visual classes or between sexes.
Nearly Singular Integrals in 3D Stokes Flow
Authors: Tlupova Svetlana , Beale J. Thomas 
Published: June 2013

AbstractA straightforward method is presented for computing three-dimensional Stokes flow, due to forces on a surface, with high accuracy at points near the surface. The flow quantities are written as boundary integrals using the free-space Green’s function. To evaluate the integrals near the boundary, the singular kernels are regularized and a simple quadrature is applied in coordinate charts. High order accuracy is obtained by adding special corrections for the regularization and discretization errors, derived here using local asymptotic analysis. Numerical tests demonstrate the uniform convergence rates of the method.
Arbitrary-Lagrangian-Eulerian One-Step WENO Finite Volume Schemes on Unstructured Triangular Meshes
Authors: Boscheri Walter , Dumbser Michael 
Published: June 2013

AbstractIn this article we present a new class of high order accurate Arbitrary-Eulerian-Lagrangian (ALE) one-step WENO finite volume schemes for solving nonlinear hyperbolic systems of conservation laws on moving two dimensional unstructured triangular meshes. A WENO reconstruction algorithm is used to achieve high order accuracy in space and a high order one-step time discretization is achieved by using the local space-time Galerkin predictor proposed in. For that purpose, a new element-local weak formulation of the governing PDE is adopted on moving space-time elements. The space-time basis and test functions are obtained considering Lagrange interpolation polynomials passing through a predefined set of nodes. Moreover, a polynomial mapping defined by the same local space-time basis functions as the weak solution of the PDE is used to map the moving physical space-time element onto a space-time reference element. To maintain algorithmic simplicity, the final ALE one-step finite volume scheme uses moving triangular meshes withstraightedges. This is possible in the ALE framework, which allows a local mesh velocity that is different from the local fluid velocity. We present numerical convergence rates for the schemes presented in this paper up to sixth order of accuracy in space and time and show some classical numerical test problems for the two-dimensional Euler equations of compressible gas dynamics.
Determination of Ochratoxin A in Feed by Immunoaffinity Cleanup and Liquid Chromatography
Authors: Ding Ping , Mi Ziyou , Hou Yali , He Yigang , Xie Jianhua 
Published: June 2013

Abstract
A method using LC was developed for determination of ochratoxin A (OTA) in feeds. The extracted samples were cleaned up by an immunoaffinity column prepared by covalently coupling polyclonal antibodies against OTA to cyanogen bromide-activated Sepharose 4B. The eluates were determined by LC with fluorescence detection. Recoveries of OTA from fortified samples of 1–10 μg/kg levels ranged from 84.3 to 90.0%, with CVs of 3.3–7.8%. The detection limit was 0.045 μg/kg based on an S/N of 3:1. A total of 65 feed samples were screened for OTA with the proposed method. The results showed that only nine samples were contaminated with OTAs at low levels. The presented method was successfully applied to quantify OTAs in real feed samples.
Validation of Artemisia Genus-Specific Gene, GEL4, for Use as an Endogenous Reference Gene in Qualitative PCR Detection of Putative Transgenic Artemisia annua
Authors: Liu Hua , Jiang Lingxi , Tan Furong , Zhao Xinghai , Lin Heyan , Zhu Hong , Wang Jinbin , Zhao Kai , Li Peng , Pan Aihu , Jia Junwei , Tang Xueming 
Published: June 2013

Abstract
Artemisia annua L. is a popular medical plant used against malaria. Transgenic A. annua plants have been developed to increase the artemisinin content using genetic engineering. To develop a reliable PCR method of detecting transgenic A. annua, one Artemisia specific gene, GEL4, was selected and validated as suitable. Qualitative PCR methods were assayed with 16 different A. annua ecotypes and four Artemisia plants, and identical amplified products were obtained. No amplified products were observed when genomic DNA samples were extracted from 15 different plant species. An LOD of 0.05 ng of A. annua DNA was also determined. Furthermore, the results of a Southern blot method showed that the GEL4 gene was in three copies of the six A. annua ecotypes tested. To determine whether there was intraspecific variability in the GEL4 region between different A. annua ecotypes, the GEL4 region corresponding to the 10 A. annua ecotypes was cloned and sequenced. Alignment of the obtained sequences presented a highly conserved amplification product region in 10 A. annua ecotypes. Therefore, it is concluded that the GEL4 gene can be used as an endogenous reference gene of A. annua, and that the qualitative PCR system was reliable for the detection of transgenic A. annua.
Evaluation of a Commercial ELISA for Detection of Ruminant Processed Animal Proteins in Non-Ruminant Processed Animal Proteins
Authors: Bremer Maria G E G , Margry Rob J C F , Vaessen Judith C H , Van Doremalen Anke M H , Van Der Palen Johan G P , Van Kaathoven Robert G C , Kemmers-Vonken Anniek E M , Van Raamsdonk Leo W D 
Published: June 2013

Abstract
Due to a growing aquaculture industry, demand for high-quality proteins for aquatic feeds is increasing. Non-ruminant processed animal proteins (PAPs) have shown great potential for this purpose. Safe reintroduction of non-ruminant PAPs in aqua feed requires methods that can discriminate ruminant and non-ruminant PAPs at contamination levels at or below 2%. Because the official European Union method lacks species specificity, the performance of MELISA-TEK™ Ruminant, a commercial immunoassay, combined with the MELISA-TEK High Sensitivity Sample Extraction kit was evaluated. Various non-ruminant PAPs spiked with ruminant PAPs (processed at 133, 137, 141, and 145°C) were analyzed. Results showed an overall specificity of 99%, indicating no cross-reaction with non-ruminant PAPs. The sensitivity of the assay strongly depended on both processing temperature and proportion of muscle fibers of the ruminant PAPs. Overall sensitivity of samples with 1 and 2% ruminant PAPs was 92 and 100%, respectively. For ruminant PAPs processed at 133 and 137°C, the sensitivity was 100% for both 1 and 2% ruminant spikes. Overall accuracies were 96 and 99% for 1 and 2% ruminant spikes, respectively. In conclusion, the MELISA-TEK Ruminant assay showed satisfactory results, which makes it a suitable candidate method to enable safe reintroduction of non-ruminant PAPs in aqua feed.
Rapid Determination of Chloramphenicol in Honey by Laser Diode Thermal Desorption Using Atmospheric Pressure Chemical Ionization-Tandem Mass Spectrometry
Authors: Blachon Grégory , Picard Pierre , Tremblay Patrice , Demers Sarah , Paquin Réal , Babin yves , Fayad Paul B 
Published: June 2013

Abstract
A high-throughput, rapid, and reliable method based on laser diode thermal desorption (LDTD) and atmospheric pressure chemical ionization coupled to tandem MS (APCI-MS/MS) was used to identify and quantify chloramphenicol (CAP) residues in honey. Sample pretreatment consisted of a liquid–liquid extraction of diluted honey in water with ethyl acetate containing stearic acid. After extraction, a 2 μL aliquot of the organic phase was deposited into a 96-well plate prior to detection by LDTD-APCI-MS/MS. The total analysis time was less than 6 s compared to several minutes for traditional chromatographic methods used for CAP detection, since no chromatographic separation was necessary. The extraction and analysis were made with honey samples of different color grading ranging from extra white to dark amber. The method showed good linearity (R2 = 0.99995) within a concentration range of 0.1 to 500 ng/g for CAP with D5-CAP as an internal standard. The RSD varied between 8 and 24% over the calibration range (n = 4). The method LOD for CAP in honey was 0.19 ng/g. This work demonstrates that LDTD-APCI-MS/MS could be used for fast and effective quantification of CAP in honey samples.
Online Preconcentration System Using 6-(2-Thienyl)-2-Pyridine Carboxaldehyde Functionalized Amberlite XAD-4 Resin for the Determination of Trace Elements in Waters by Flame Atomic Absorption Spectrometry
Authors: Karadaş Cennet , Kara Derya 
Published: June 2013

Abstract
An online flow injection method was developed for the determination of trace elements in waters using 6-(2-thienyl)-2-pyridinecarboxaldehyde functionalized Amberlite XAD-4 resin. The metals were eluted with 1.0 M HNO3 directly to a flame atomic absorption spectrometry determination system. The sorption capacities of the resin for metal ions were 74.6, 113.7, 48.3, 131.6, and 88.6 μmol/g for Cd(II), Ni(II), Pb(II), Co(II), and Cu(II), respectively. The resin was found to be very stable and capable of being used for more than 250 retention and elution cycles without major loss of performance. Detection limits (3SD) were determined to be 0.14 μg/L for Cd, 0.39 μg/L for Cu, 0.82 μg/L for Ni, 0.88 μg/L for Co, and 2.54 μg/L for Pb using 10 mL sample loading. The accuracy of the proposed method was checked using different certified reference materials [estuarine water (SLEW-3), riverine water (SRLS-5), 19-element aqueous standard (QCS-19), wheat flour (NBS 1567 a), and rice flour unpolished high level Cd (NIES 10c)]. Results were in agreement with certified values. The proposed method was applied to the determination of Cu, Ni, Co, Cd, and Pb in natural water samples. The recovery values for spiked water samples were between 91.9 and 107.4%.
Fatty Acid Analysis of Saw Palmetto (Serenoa repens) and Pygeum (Prunus africana) in Dietary Supplements by Gas Chromatography/Mass Spectrometry in the Selected Ion Monitoring Mode
Authors: Wang Mei , Avula Bharathi , Wang Yan-Hong , Zhao Jianping , Parcher Jon F , Khan Ikhlas A 
Published: June 2013

Abstract
A GC/selected ion monitoring mode-MS (GC/SIM-MS) method was developed to chemically distinguish saw palmetto and pygeum in dietary supplements. A sample set including authenticated plant samples, commercial plant extracts, a National Institute of Standards and Technology standard reference material extract, and commercial dietary supplements purported to contain either saw palmetto, pygeum, or both was investigated. Fatty acid methyl esters (FAMEs) were analyzed by GC/SIM-MS. The ability to selectively monitor each solute allowed accurate quantitation of all detected FAMEs, even those that were not chromatographically resolved. The major fatty acid components that were common to both saw palmetto and pygeum were oleic, linoleic, and palmitic acids. The major component characteristic of pygeum was stearic acid. Lauric and myristic acids were observed predominantly in saw palmetto samples. Principal component analysis was used for interpretation of the analytical results. A distinct cluster was observed for the samples containing pygeum alone. A separate cluster was observed for the samples containing saw palmetto alone or mixed with pygeum. The proposed GC/MS method is useful for chemical fingerprint analysis and QC of dietary supplements claiming to contain pygeum and/or saw palmetto.
Development and Validation of a Method for the Simultaneous Determination and Confirmation of Melamine and Cyanuric Acid in Fish Kidney by LC/MS/MS
Authors: Nochetto Cristina B , Stine Cynthia B , Reimschuessel Renate 
Published: June 2013

Abstract
A method was validated to simultaneously determine and confirm melamine and cyanuric acid in fish kidneys by LC/MS/MS. This method is capable of detecting both compounds in a single procedure, whether present as free compounds or bound together as the melamine–cyanurate complex in both channel catfish (Ictalurus punctatus) and rainbow trout (Oncorhynchus mykiss) kidneys. Residues are extracted with no additional cleanup and analyzed by LC/MS/MS using external standard calibration. The method is capable of quantifying residues over a range of 0.4 to 50 μg/g. For both compounds and species of fish tested, the LOD is estimated to be 0.1 μg/g and the LOQ 0.4 μg/g. Recoveries and RSDs are 83 to 101% and 2 to 8%, respectively. In catfish, matrix effects are higher for melamine than cyanuric acid. In rainbow trout, similar matrix effects are found for both compounds.
iQ-Check Listeria spp. Real-Time PCR Test Kit
Authors: Lauer Wendy F , Tourniaire Jean-Philippe 
Published: June 2013

Abstract
A comparative evaluation study of the Bio-Rad® iQ-Check™Listeria species Kit (Bio-Rad Laboratories, Hercules, CA) was conducted at Q Laboratories, Inc., Cincinnati, OH. iQ-Check is a rapid method based on real-time PCR amplification and detection of all species of Listeria, including L. grayi, in food and environmental samples. The iQ-Check method was compared to the Health Canada MFHPB-30 reference method for the analysis of five ready-to-eat meats—deli turkey, hot dogs, liver paté, raw fermented sausage, and deli ham—and one stainless steel surface. Each food matrix was analyzed at two contamination levels: a low level at 0.2–2 CFU/25 g and a high level at 2–5 CFU/25 g. The environmental surfaces were analyzed at a low level of 0.2–2 CFU/5 cm2 sampling area and a high level of 2–5 CFU/5 cm2 sampling area. There were 20 replicates per contamination level and five control replicates at 0 CFU/25 g or 0 CFU/5 cm2 sampling area (uninoculated). All samples that were detected by iQ-Check were subsequently confirmed by reference method protocol. There was no significant difference in the number of positive samples detected by the iQ-Check Listeria spp. Kit in comparison to the Health Canada MFHPB-30 method for all matrixes tested.
Preparation and characterization of polylactide nanofibers via melt extrusion of polylactide/copolyester blends
Authors: Liu Peng , Zhu Keying , Ouyang Yang , Xiao Ru 
Published: June 2013

ABSTRACTIn this article, polymer blends of polylactide (PLA) and co‐polyester (co‐PET) were prepared at various weight ratios of PLA/co‐PET, such as 10/90, 20/80, 30/70, and 40/60, through a twin‐screw extruder. The PLA nanofibers were fabricated by removal of the co‐PET matrix in water at 80°C. The morphology development of PLA dispersed phase obtained from the three different sample connections and the die of the twin‐screw extruder were investigated by Scanning Electron Microscopy (SEM). It was found that the uniformed PLA nanofibers with averaged diameters less than 500 nm were fabricated by the suitable processing parameters. The processing immiscibility and rheological behavior of PLA/co‐PET blends were also studied by means of Differential Scanning Calorimeter (DSC) and Capillary Rheometer. The test of Fourier Transform Infrared spectroscopy (FTIR) demonstrated that the co‐PET was removed clearly in water at 80°C. © 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 130: 2832–2838, 2013