bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinical characteristics and effects of GH replacement therapy in adults with childhood-onset craniopharyngioma compared with those in adults with other causes of childhood-onset hypothalamic–pituitary dysfunction
Authors: Yuen Kevin C J , Koltowska-Häggström Maria , Cook David M , Fox Janet L , Jönsson Peter J , Geffner Mitchell E , Abs Roger 
Published: August 2013

ObjectiveAdults with childhood-onset (CO) craniopharyngioma (COCP) have poor quality of life (QoL) and clinical outcomes, but few studies have compared these patients with adults with other causes of CO hypothalamic–pituitary dysfunction. In this study, we compared baseline clinical characteristics and patient-reported outcomes before starting GH replacement therapy in adults with GH deficiency (GHD) due to COCP with those of adults either with CO idiopathic/congenital hypopituitarism (COH) or with CO extrasellar (COE) tumours, and evaluated the 1- and 5-year effects of GH replacement therapy.Subjects and methodsRetrospective analysis of the data recorded in KIMS (Pfizer International Metabolic Database) was carried out. Patients with COCP, COH and COE tumours were evaluated at baseline, and after 1 and 5 years of therapy.ResultsCompared with COH and COE patients, more COCP patients underwent surgery, had greater abnormalities of body composition and higher prevalence of pituitary hormone deficits (allP<0.001), but comparable fasting glucose, HbA1c, total cholesterol and LDL-cholesterol levels, marital status, parenthood, living arrangements, education, employment and annual sick-leave days. After 1 and 5 years of GH replacement therapy, similar changes were evident with regard to body composition, fasting glucose and HbA1c levels, QoL, and the level of and satisfaction with physical activity across the three groups.ConclusionsAdults with untreated COCP with GHD at baseline demonstrated more co-morbidities including greater abnormalities of body composition, pituitary hormone deficits and visual field defects. Overall, adults with COCP, COH and COE tumours responded comparably to short- and long-term GH replacement therapy, suggesting that patients with GHD due to COCP benefited from GH replacement therapy to a similar degree as those with other causes of CO hypothalamic–pituitary dysfunction did.
AMP-activated protein kinase signaling is upregulated in papillary thyroid cancer
Authors: Vidal Ana Paula , Andrade Bruno M , Vaisman Fernanda , Cazarin Juliana , Pinto Luis Felipe Ribeiro , Breitenbach Marisa Maria Dreyer , Corbo Rossana , Caroli-Bottino Adriana , Soares Fernando , Vaisman Mario , Carvalho Denise P 
Published: August 2013

AMP-activated protein kinase (AMPK) is activated by the depletion in cellular energy levels and allows adaptive changes in cell metabolism and cell survival. Recently, our group described that AMPK plays an important role in the regulation of iodide and glucose uptake in thyroid cells. However, AMPK signaling pathway in human thyroid carcinomas has not been investigated so far.ObjectiveTo evaluate the expression and activity of AMPK in papillary thyroid carcinomas.MethodsWe examined total and phosphorylated AMPK (tAMPK and pAMPK) and phosphorylated acetyl-CoA-carboxylase (pACC) expressions through imunohistochemistry, using a tissue microarray block composed of 73 papillary thyroid carcinomas (PAP CA) or microcarcinomas (PAP MCA) and six adenoma (AD) samples from patients followed at the Federal University Hospital. The expression levels were compared with the non-neoplastic tissues from the same patient. Two different pathologists analyzed the samples and attributed scores of staining intensity and the proportion of stained cells. A total index was obtained by multiplying the values of intensity and the proportion of stained cells (INTxPROP).ResultstAMPK, pAMPK, and pACC showed a predominant cytoplasmic staining in papillary carcinomas, adenomas, and non-neoplastic thyroid tissues. However, the intensity and the proportion of stained cells were higher in carcinomas, so that a significant increase was found in the INTxPROP score both in PAP CA and PAP MCA, when compared with their respective controls.ConclusionOur results show unequivocally that AMPK pathway is highly activated in papillary thyroid carcinomas; however, more studies are necessary to understand the pathophysiological significance of AMPK activation in thyroid carcinogenesis.
Influence of ovarian manipulation on reproductive endocrinology in polycystic ovarian syndrome and regularly cycling women
Authors: Hendriks M L , König T , Soleman R S , Korsen T , Schats R , Hompes P G A , Homburg R , Lambalk C B 
Published: August 2013

ObjectiveLittle is known about the function of the ovarian neuronal network in humans. In many species, copulation influences endocrinology through this network. As a first step, the possible influence of ovarian mechanical manipulation on pituitary and ovarian hormones was evaluated in polycystic ovarian syndrome (PCOS) and regularly cycling women. DesignProspective case–control study (2008–2010).MethodsTen PCOS women (Rotterdam criteria) undergoing ovulation induction with recombinant-FSH and ten normal ovulatory controls were included in an academic fertility clinic. In the late follicular phase blood was drawn every 10 min for 6 h. After 3 h the ovaries were mechanically manipulated by moving a transvaginal ultrasound probe firmly over each ovary ten times. Main outcome measures were LH and FSH pulsatility and ovarian hormones before and after ovarian manipulation.ResultsAll PCOS patients showed an LH decline after the ovarian manipulation (before 13.0 U/l and after 10.4 U/l, P<0.01), probably based on a combination of a longer LH pulse interval and smaller amplitude (P=0.07). The controls showed no LH change (before 9.6 U/l and after 9.3 U/l, P=0.67). None of the ovarian hormones (estradiol, progesterone, anti-Müllerian hormone, inhibin B, androstenedione and testosterone) changed in either group.ConclusionsOvarian mechanical manipulation lowers LH secretion immediately and typically only in preovulatory PCOS patients. The immediate LH change after the ovarian manipulation without any accompanying ovarian hormonal changes point to nonhormonal communication from the ovaries to the pituitary. A neuronal pathway from the ovaries communicating to the hypothalamic–pituitary system is the most reasonable explanation.
Effect of Cementation Technique of Individually Formed Fiber-Reinforced Composite Post on Bond Strength and Microleakage
Authors: Makarewicz Dominika , Le Bell-Rönnlöf Anna-Maria B , Lassila Lippo V.J. , Vallittu Pekka K. 
Published: August 2013

Objectives:
The aim of this study was to evaluate the effect of two different cementation techniques of individually formed E-glass fiber-reinforced composite (FRC) post on bond strength and microleakage.

Methods:
The crowns of extracted third molars were removed and post preparation was carried out with parapost drills (diameter 1.5 mm). After application of bonding agents individually formed FRC posts (everStick POST, diameter 1.5 mm) were cemented into the post spaces with either ParaCem®Universal or self-adhesive RelyX™Unicem, using two different cementation techniques: 1) an “indirect (traditional) technique” where the post was prepolymerized prior application of luting cement and insertion into the post space or 2) a “direct technique” where the uncured post was inserted to the post space with luting cement and light-polymerized in situ at the same time. After water storage of 48 hours, the roots (n = 10/group) were cut into discs of thickness of 2 mm. A push-out force was applied until specimen fracture or loosening of the post. A microleakage test was carried out on roots which were not subjected to the loading test (n= 32) to evaluate the sealing capacity of the post-canal interface. The microleakage was measured using dye penetration depth under a stereomicroscope.

Results:
Higher bond strength values (p<0.05) and less microleakage (p<0.05) were obtained with the “direct technique” compared to the “indirect technique”. None of the FRC posts revealed any dye penetration between the post and the cement.

Conclusions:
The “direct technique” seems to be beneficial when cementing individually formed FRC posts.
Identification of Telomeric Sequences in Pigs with Rearranged Karyotype Using Prins Technique / Identyfikacja sekwencji telomerowych u świń z rearanżacją kariotypu przy wykorzystaniu techniki PRINS
Authors: Danielak-Czech Barbara , Rejduch Barbara , Kozubska-Sobocińska Anna 
Published: August 2013

Abstract Using PRINS technique with oligonucleotide primer complementary to the telomere sequence repeats (TTAGGG )n, the interstitial telomeric signals in 6q22 and 7q13 unstable chromosome regions in pigs with t(7;13)(q13;q46) reciprocal translocation were identified. The intrachromosomal localization of telomeric repeats specific for 7q13 region were revealed to strictly correspond to breakpoint of this translocation as well as several others described earlier. The present study showed the usefulness of PRINS technique for identification of intrachromosomal telomeric sequences in pig genome regions, extremely prone to breakage and predisposed to chromosome reorganizations, especially with regard to the clinical cases of karyotype defects.
Temperature, Humidity and Air Movement Variations Inside a Free-Stall Barn During Heavy Frost / Zróżnicowanie warunków cieplno-wilgotnościowych i ruchu powietrza w oborze wolnostanowiskowej podczas silnych mrozów
Authors: Herbut Piotr 
Published: August 2013

Abstract The paper presents results of research on the variability of temperature, humidity and air movement in selected areas of a free-stall barn during heavy frost. The study revealed the occurrence of unfavourable factors influencing microclimate in the barn. Some areas of the barn suffered from draughts, some were excessively exposed to sun or too shaded, which resulted in significant temperature and relative humidity fluctuations during the day as well as changes in air movement velocity. All these factors exerted an impact on cattle welfare. The conclusion points out the need to determine zones in the barn characterized by clearly different microclimatic conditions. In the studied example, these conditions were influenced by weather changes and depended on the orientation of the building to cardinal points as well as wall construction materials.
Stimulatory Effect of hCG on Male American Mink (Neovison Vison) in the Breeding Season / Wpływ stymulacji hCG na samce norki amerykańskiej (Neovison vison) w okresie kryć
Authors: Lasota Bogdan , Masłowska Anna , Felska-Błaszczyk Lidia , Dziadosz Małgorzata , Seremak Beata , Skuratko Agata 
Published: August 2013

Abstract The aim of the study was to analyse the effect of a male mink single-dose hCG stimulation on the libido during the mating season and on blood testosterone levels after the season. The material involved males of American mink. The treatment-group males were administered a dose of 100, 150 or 200 U of hCG. Blood was collected twice, approx. 2 weeks prior to and on the completion of the mating season. The group receiving 100 U hCG had the highest percentage of males effectively copulating with females within the first 24 hours after stimulation, whereas males stimulated with a dose of 200 U hCG showed the lowest libido over the same period. On the other hand, males of the group stimulated with 150 U hCG mated to the highest number of females throughout the mating season. The mean plasma testosterone concentration in all the studied males on 18 February was 12.44 ng/ml. The drop in testosterone concentration at the end of the mating season was significant.
A Connection Between Mastitis During Early Lactation and Reproductive Performance of Dairy Cows – A Review / Związek mastitis we wczesnej laktacji z użytkowością reprodukcyjną krów mlecznych – artykuł przeglądowy
Authors: Olechnowicz Jan , Jaśkowski Jędrzej M. 
Published: August 2013

Abstract The objective of this study was to present a relationship between mastitis in the post-calving period and fertility traits of dairy cows. The threshold of 200,000 cells/ml for somatic cell count is used as a reference for healthy quarters of the cow’s udder. The genetic correlation between mastitis and somatic cell count is strong (from 0.7 to 0.8). Although heritability for fertility traits is low (from 0.01 to 0.02), the genetic standard deviation for mastitis varies from 1.2 to 7.0 percentage units, suggesting that genetic gain can be achieved by selecting for mastitis. Results of this study suggest that mastitis in the postpartum period can have a markedly negative impact on the reproductive performance of dairy cows. The synergistic effect of common conditions (somatic cell count, body condition score and lameness score) or other factors (e.g. heat stress, fertility management, the presence of repeat breeders) also lowers fertility of cows. Production of milk with fewer than 100,000 cells/ml leads to improved health and fertility in the cows.
The Relationship Between CRP Gene Polymorphism and the Serum Concentrations of C-Reactive Protein, Total Cholesterol and HDL Cholesterol in Suckling Piglets / Związek polimorfizmu genu CRP z koncentracją kodowanego białka C-reaktywnego, cholesterolu całkowitego i HDL-ch w surowicy krwi ssących prosiąt
Authors: Łaszyn Marek , Sielawa Hanna , Życzko Krystyna 
Published: August 2013

AbstractThe relationship between CRP gene (1271 G/A, 3’UTR) polymorphism and the serum levels of C-reactive protein (CRP), total cholesterol (Ch-T) and high density lipoprotein cholesterol (HDL-ch) was analysed in suckling crossbred [Polish Large White × Polish Landrace (♀) × × Duroc × Pietrain (♂)] piglets. CRP genotypes were identified by PCR-RFLP with Hinfi restriction enzyme. The levels of CRP, Ch-T, HDL-ch and white blood cell (WBC) counts were determined in blood samples collected from younger (21±3 days of age) and older piglets (35±3 days of age). There was a relationship between CRP gene (1271 G/A, 3’UTR) polymorphism and variations in the serum levels of CRP in piglets with normal WBC counts. The above relationship did not manifest itself in piglets with elevated WBC counts. The studied genotypes differed in their response to elevated WBC counts, and the noted differences were more pronounced in older piglets. The response of genotypes with weak CRP expression caused an increase in CRP levels and a decrease in the serum concentrations of Ch-T and HDL-ch. Such a response was not observed in the genotype with strong CRP expression.
Hygienic Aspects of Cattle Slurry Storage as the Most Popular and Cheapest Method of Handling Liquid Animal Excrements / Higieniczne aspekty składowania gnojowicy bydlęcej jako najpopularniejszej i najtańszej metody postępowania z płynnymi odchodami zwierzęcymi
Authors: Skowron Krzysztof , Olszewska Halina , Skowron Karolina Jadwiga , Paluszak Piotr , Breza-Boruta Barbara 
Published: August 2013

Abstract Slurry is a very valuable natural fertilizer, but its improper use in agriculture poses a serious sanitary threat. Therefore its treatment before use for fertilization is advisable. One of such methods, which is still the most popular, is storage of liquid excrements. The aim of this study was to estimate the sanitization effectiveness of storage at 4ºC and 20ºC based on parameters describing the kinetics of changes in the population of some indicator bacteria in cattle slurry with different dry matter content. The material for the study was fresh cattle slurry. The liquid excrements used in the experiment had a dry matter content of 2, 6 and 14%. Slurry was stored at 4ºC and 20ºC. Bacilli of Salmonella Dublin, E. coli and enterococci were used as indicator bacteria. Number of microorganisms was determined based on MPN method in a 3-tube design. Basic parameters of the bacteria inactivation kinetics were calculated and statistical analysis was made using the program SAS 9.2 PL. In stored slurry a gradual elimination of all the studied microorganisms was observed. Hygienization effect of storage was smaller at 4ºC than at 20ºC and in excrements with a high dry matter content. Depending on storage temperature and dry matter proportion, the theoretical times of survival ranged from 81.85 to 220.80 days for bacilli of Salmonella Dublin, from 74.93 to 199.36 days for E. coli, and from 118.67 to 335.84 days for enterococci. The study showed explicitly that statutory time of slurry storage is insufficient to ensure its complete hygienization.
Poultry Meat as Functional Food: Modification of the Fatty Acid Profile – A Review / Mięso drobiowe jako żywność funkcjonalna: modyfikacja profilu kwasów tłuszczowych – artykuł przeglądowy
Authors: Zduńczyk Zenon , Jankowski Jan 
Published: August 2013

Abstract Functional foods, defined as “foods that may provide health benefits beyond basic nutrition”, became increasingly popular in the past twenty years with numerous practical applications. In Europe, functional foods must be accompanied by scientifically substantiated health claims. Products which aspire to that category include poultry meat and processed meat products which have been modified through bird nutrition. This article reviews the existing knowledge about foods fortified with health-promoting additives. It discusses the physiological, economic and legal aspects of modifying poultry meat, including turkey meat which has been poorly investigated in this context. The addition of oils rich in PUFA (polyunsaturated fatty acids), e.g. linseed oil, to poultry diets has been found to increase LC n-3 PUFA (long-chain omega-3 PUFA) concentrations in chicken and turkey meat. LC n-3 PUFAs participate in many processes that condition metabolism and health, and the nutritional value of meat, including poultry, is most commonly enhanced by increasing the proportion of LC n-3 PUFAs in the product's fatty acid composition. However, it increases feed costs and may cause a deterioration in the sensory attributes and oxidative stability of meat. Turkey breast meat is characterized by a relatively low fat content, which is why the fulfilment of health claim requirements is difficult in the European Union.
Growth, yielding and susceptibility to fire blight of cider apples in the climatic conditions of Central Poland
Authors: Czynczyk Alojzy , Chlebowska Danuta , Epps Tim 
Published: August 2013

Abstract
The possibility of growing 8 cider apple cultivars on M.26, P 14 and Antonovka seedlings in the climatic conditions of Central Poland was studied over a period of 7 years (1996 - 2003). The results obtained showed that cultivars of cider apples are more susceptible to fire blight than standard cultivars ‘James Grieve’, ‘Wealthy’ and ‘Common Antonovka’. After seven years of growth, the most vigorously growing cultivars on the three rootstocks were ‘Ellis Bitter’ and ‘Major’, and on Antonovka seedlings also ‘Yarlington Mill’ and ‘Harry Masters’ Jersey’. The weakest growth was shown by ‘Ashton Bitter’ and ‘Michelin’ on all three rootstocks. Trees of cider cultivars ‘Dabinett’, ‘Michelin’, ‘Ashton Bitter’ and ‘Chisel Jersey’ were similar in size to standard cultivar ‘James Grieve’. After the seven-year period, the highest cumulative yields were obtained from ‘Dabinett’, ‘Chisel Jersey’ and ‘Yarlington Mill’ grown on all three rootstocks. A high cumulative yield was also obtained from ‘Major’ grafted on M.26 and P 14 and ‘Michelin on P 14 and Antonovka seedlings. ‘Dabinett’, ‘Major’ and ‘Chisel Jersey’ were high productive cultivars and can be recommended for growing on P 14 and Antonovka seedlings in commercial orchards in Poland.
Pathogenicity of selected soil-borne microorganisms for scorzonera seedlings (Scorzonera hispanica L.)
Authors: Patkowska Elżbieta , Konopiński Mirosław 
Published: August 2013

Abstract
The pathogenicity of A. alternata, F. culmorum, F. oxysporum, F. solani, P. irregulare, and R. solani isolated from oats, spring vetch, and tancy phacelia mulch soil to Scorzonera hispanica L. in the conditions of a growth chamber was studied. The pathogenicity of these microorganisms was established on the basis of the number of emerged plants and their healthiness. The studied species considerably decreased the emergencies and healthiness of the seedlings, e.g. necrosis of sprouts and roots. Differentiated harmfulness of particular microorganisms isolates was observed within a given species. The isolates of P. irregulare and F. oxysporum proved to be the most severe for the seedlings, while A. alternata the least.
Lavandula angustifolia Mill. as a natural host of Cucumber mosaic virus (CMV)
Authors: Kobyłko Tadeusz , Dańda Piotr , Hasiów Beata , Pospieszny Henryk , Borodynko Natasza 
Published: August 2013

Abstract
A virus was isolated from Lavandula angustifolia Mill. plants exhibiting yellow mottling and distortion of leaves. After mechanical inoculation it induced in the major part of used test plants symptoms characteristic for Cucumber mosaic virus (CMV). Its standard properties regarding the stability in crude plant sap were as follows: longevity in vitro 1-2 days, thermal inactivation point 55-60°C, dilution end point log10minus 3 - 4. The virus reacted positive with diagnostic antiserum against CMV in DAS-ELISA test. RT-PCR reaction revealed similarity between the investigated isolate and the isolate of CMV from the Netherlands belonging to subgroup II. In the light of the foregoing facts the isolated pathogen can be identified as the Cucumber mosaic virus and Lavandula angustifolia may be regarded as its natural host.
Pathogenicity of fungi colonising the soil after the cultivation of cover crops towards the seedlings of salsify Tragopogon porrifolius var. sativus (Gaterau) Br.
Authors: Patkowska Elżbieta , Konopiński Mirosław 
Published: August 2013

Abstract
The purpose of the present study was to conduct pathogenicity tests for salsify seedlings in a soil environment colonised by selected fungi species, after the cultivation of cover crops such as oats, common vetch and tancy phacelia. A growth chamber experiment used the seeds of ‘Mamut’ salsify and soil overgrown with Altenaria alternata, Fusarium culmorum, F. oxysporum, Pythium irregulare, Rhizoctonia solani and Sclerotinia sclerotiorum. The pathogenicity of these microorganisms towards salsify seedlings was established on the basis of the grown plants, their health and a disease index calculated on the basis of a fivedegree scale. The examined isolates considerably weakened the emergence and health of salsify. Based on pathogenicity tests, such species as R. solani, S. sclerotiorum and P. irregulare were considered as the major cause of seedling necrosis and root rot of the plants of the Tragopogon porrifolius var. sativus. Among the studied soil-borne plant pathogens, the most pathogenic ones towards salsify seedlings proved to be the isolates of R. solani, S. sclerotiorum and P. irregulare, whereas the least harmful included A. alternata and F. culmorum.
The morphogenetic capability and the viability of regenerants in micropropagated orchid hybrids infected with viral pathogens
Authors: Cybularz-Urban Teresa , Hanus-Fajerska Ewa 
Published: August 2013

Abstract
The micropropagation efficiency of four interspecific Cattleya hybrids (clones: 69, 75, 149 and 150) infected with Cymbidium mosaic (CyMV) and Odontoglossum ringspot (ORSV) viruses was assessed. The aim of experiments was to evaluate with that model to what extent viral infection affects the morphogenesis in vitro in orchid hybrids of different origin. The effectiveness of plant material exposure to therapeutic levels of plant growth regulators supplied with media in order to suppress infection was also verified. The vitality of proliferating infected shoot cultures was limited, and the symptoms of senility were frequently observed. Regardless genotype of the studied clone, during acclimation to ex vitro conditions considerable losses become visible what indicates the necessity of testing the donor material for possible latent viral infections. Infection with CyMV and ORSV mostly persisted in every tested clone.