bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Unique Case of Cutaneous Calcinosis with Transepidermal Elimination
Authors: Majima Makiko , Kanai Takako , Oh‐i Tsunao 
Published: April 2015

AbstractA case of cutaneous calcinosis with unique clinical and histological features which occurred on the cheek of a 14‐year‐old girl, is reported. Our case had no abnormal findings in laboratory data. Serum calcium and phosphate were normal, there was no underlying disease, and the possibility of self‐inflicted dermatoses was denied. Transmission electron microscopy and X‐ray microanalysis showed calcium and phosphate deposited around collagen fibers, which were eliminated from the epidermis.
Trichilemmal Cyst: Report of a Case in Which the Cyst Was Connected with the Epidermis by an Epidermal Canal
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

AbstractAn 87‐year‐old Japanese woman with a trichilemmal cyst (TC) on the subnasal area is described. Histopathological examination revealed that the cyst was connected with the overlying epidermis by an epidermal canal. This has suggested that the pathogenesis of TCs could be that they originate from the isthmus or lower portion of the hair follicle.
Blood Vessels and Immunoreactive Substance P‐Containing Nerve Fibers in Rat Skin Treated Topically with Clobetasol Propionate, a Corticosteroid
Authors: Oura Hajimu , Takeda Katsuyuki , Daikoku Shigeo 
Published: April 2015

AbstractAfter applying topically a cream (0.1 ml) containing corticosteroid (clobetasol propionate), on rat back skin, we examined the morphological alterations of blood vessels, substance P‐containing nerve fibers, and cutaneous mast cells. After 3, 6, 10, 15, 30, and 60 min and 4 h, the skin treated was cut out with a sharp knife after killing the animals. The skin pieces were processed into conventional histological sections cut vertically and examined by staining immunohistochemically with anti‐substance P serum, by staining with toluidine blue for mast cell granules, and by estimating morphometrically the average areas of vascular cavity and the number of substance P fibers in the dermis. In the dermis and subcutaneous tissue of untreated skin, we found many immunoreactive SP‐containing nerve fibers and mast cells in close association. Three to ten min after the treatment, the average area of the vascular cavities steadily increased, and SP‐positive fibers became less frequent in the dermis. In concomitant with those events, cutaneous mast cells discharged their granules. Thereafter, the average area of vascular cavities gradually decreased to a minimum at 4 h after the treatment. In contrast, both SP‐containing fibers and mast cells reestablished their initial states after the same duration.
Photosensitivity in Atopic Dermatitis: Demonstration of Abnormal Response to UVB
Authors: Keong Chin‐Huai , Kurumaji Yuko , Miyamoto Chizu , Fukuro Shuhei , Kondo Seiji , Nishioka Kiyoshi 
Published: April 2015

AbstractIt is a well‐known fact among clinicians that sunlight may exacerbate atopic dermatitis (AD), but little is known beyond that. In a preliminary study investigating this phenomenon, 19 patients with AD were selected for phototests. All of them had a normal minimal erythema dose (MED). However, 3 patients (15.7%) demonstrated abnormal cutaneous responses 24–72 h after provocation with ultraviolet light B (UVB). None of the patients had a positive response to pure ultraviolet light A (UVA) irradiation of up to 9 J/cm2. The photobiological results of this study confirm the existence of photosensitivity in AD and indicate that UVB wavelengths are responsible for it.
Triple Cancers in the Urogenital Area of a Patient with Aplastic Anemia
Authors: Sato Kazuko C. , Furuta Yasushi , Takasu Tsuyoshi , Nagashima Kazuo , Fukaya Toru , Koizumi Hiroko , Ohkawara Akira , Yamashiro Katsushige , Kawashima Kunihiro , Yoshida Tetsunori 
Published: April 2015

AbstractThree epithelial neoplastic lesions, perineal Bowenoid papulosis, uterine cervical carcinoma, and bladder transitional cell carcinoma, which occurred in a mildly immunosuppressed patient who had aplastic anemia were studied for human papillomavirus (HPV) infection. In the Bowenoid papulosis, HPV type 16 DNA was identified by polymerase chain reaction (PCR) and by in situ hybridization (ISH). In contrast, in the uterine cervical carcinoma, HPV 16 was not detected, although possibly another unidentified type of HPV in the lesion was suggested by the ISH findings. In the bladder transitional cell carcinoma, neither papillomavirus genus‐specific (PGS) antigen nor HPV DNA was found.
Ingrown Toenails: An Evaluation of Elevating the Nail Bed‐Periosteal Flap
Authors: Umeda Tadashi , Nishioka Kiyoshi , Ohara Kazunori 
Published: April 2015

AbstractMany procedures to correct ingrown toenails are available; however, they cannot avoid recurrence and nail deformities. We have performed a procedure which corrects ingrown toenail without reducing the width of the nail plate by elevating the nail bed‐periosteal flap. Sixty patients were observed for longer than six months and exhibited excellent results. Only two cases of incurvated nails (2) required re‐operation.
Lichen Planus Annularis: An Immunohistochemical Study
Authors: Ohta Yukinori , Yonemoto Kohzoh , Asai Toshiko , Yaguchi Atsushi 
Published: April 2015

AbstractLichen planus annularis is a relatively rare skin manifestation of lichen planus. The mechanisms in the formation of annular lesions are not fully understood. We reported here a 57‐year‐old female with this disease. The eruption initially occurred as lichen‐papules, then enlarged (bean‐sized, umbilicated small plaques), and finally developed annular manifestations. We performed immunohistochemical examinations of specimens taken from different types of eruptions.In all specimens, HLA‐DR was expressed in the focal keratinocytes adjacent to the dermal HLA‐DR positive cell infiltration. Both in the initial papule and in the final annular lesion, expression of ICAM‐1 was present only in the keratinocytes above the dermal cell infiltration, similar to HLA‐DR. It is of interest that, in the umbilicated small plaques, the peripheral epidermis other than the central site extensively reacted to ICAM‐1. LFA‐1 expression was most prominent in the mononuclear cells impinging on the dermo‐epidermal junction in all specimens. In addition, in the periphery of the umbilicated small plaques, which showed no bandlike dense cell infiltration nor degeneration of basement membrane, TNF‐alpha, but not LFA‐1, was positive in the infiltrated cells of the upper dermis.These results suggest that expressions of ICAM‐1 and TNF‐alpha in the peripheral keratinocytes and dermal infiltrated cells are important molecular events in the mechanisms of formation of the annular lesions.
Restriction of Cicatricial Pemphigoid Antigens to the Lamina Densa: Confirmation by Indirect Immunoelectron Microscopy
Authors: Horiguchi Yuji , Tanaka Toshihiro , Akioka Narumi , Tachibana Takao , Azumi Hiroko , Furukawa Fukumi , Imamura Sadao 
Published: April 2015

AbstractCirculating anti‐basement membrane zone (BMZ) antibodies in a patient with cicatricial pemphigoid (CP) were examined using an indirect immunofluorescence test, indirect immunoperoxidase electron microscopy, and Western blot analysis. An indirect immunofluorescence test on salt‐split skin revealed that the anti‐BMZ antibodies reacted solely to the dermal side at the separating epidermal‐dermal interface, and indirect immunoelectron microscopy on intact skin indicated localization of the corresponding antigens (CP antigens) over the lamina densa and within the lower half of the lamina lucida; there were no CP antigens beneath a melanocyte. Indirect immunoelectron microscopy on salt‐split skin demonstrated that the CP antigens were partly dissociated from, but restricted to, the lamina densa. Western blot analysis showed no differences in molecular weight between the CP antigens and bullous pemphigoid (BP) antigens. CP antigens, as detected by this patient's serum, appear to be constituted of molecules quite similar to BP antigens, but with different epitopes. CP antigens may be shed from basal cells and locate in the area of anchoring filaments, where they play a role in connecting basal cells to the underlying lamina densa.
The Comparison of Sun Protection Factor Values with Different Light Sources
Authors: Noda Toshiaki , Kawada Akira , Hiruma Masataro , Ishibashi Akira , Arai Seiichi 
Published: April 2015

AbstractSun protection factors (SPFs) were evaluated with three light sources (sunlight, a xenon arc solar simulator, and fluorescent lamps) in indoor and outdoor studies. Two types of light, UV‐A+B and UV+Visible, were obtained from the solar simulator. The untanned backs of twenty‐four healthy male volunteers were used as test sites. A broad spectrum sunscreen containing SPF 6, according to the manufacturer, was used. The sunscreen tested was applied at 2 mg/cm2. The actual SPF values were 4.8 with sunlight, 6.0 with UV‐A+B, 4.9 with UV+Visible, and 11.8 with fluorescent lamps. There were no significant differences between the SPF values with sunlight and those with the solar simulator; the SPF value for fluorescent lamps was significantly higher. The SPF with UV‐A+B of the solar simulator was similar to that with sunlight; the use of this light served to reduce pain on tested subjects. Therefore, UV‐A+B from the solar simulator seems to be the most appropriate artificial light source for evaluating sunscreens.
Proteus Syndrome: Report of the First Japanese Case with Special Reference to Differentiation from Klippel‐Trenaunay‐Weber Syndrome
Authors: Hagari Yoshitaka , Aso Miki , Shimao Shuhei , Okano Tohru , Kurimasa Akihiro , Takeshita Kenzo 
Published: April 2015

AbstractThis is the first report of a Japanese girl with Proteus syndrome. She presented with growth acceleration and precocious development of the left breast as well as macrodactyly, hemihypertrophy, a subcutaneous preaxillary mass, portwine stains, connective tissue nevi, and a depigmented macule. All these abnormalities were confined to the left side of her body. Although most of the manifestations fit those of Proteus syndrome, the presence of the portwine stains and hemihypertrophy also suggested Klippel‐Trenaunay‐Weber syndrome. The findings in our patient suggest that the most important characteristic distinguishing Proteus syndrome from Klippel‐Trenaunay‐Weber syndrome is the presence of functional abnormalities such as a growth spurt and precocious breast development. Proteus syndrome may be genetically different from the Klippel‐Trenaunay‐Weber syndrome.
Electron Microscopic X‐ray Microanalysis of Metals Deposited in Oral Mucosa
Authors: Kanzaki Tamotsu , Eto Hikaru , Miyazawa Shichiro 
Published: April 2015

AbstractA 35‐year‐old woman exhibited bluish‐brown discoloration of her buccal mucosa suggesting malignant melanoma. Histopathological examination revealed that the pigment was not melanin but caused by metal deposits. Electron microscopically, metallic particles were located on the lamina densa of basal laminae at mucosal epithelium, nerve fibers, and bloodvessels and on the microfilaments of elastic fibers as well as in macrophages and fibroblasts. Electron microscopic point X‐ray microanalysis revealed that these metallic particles were composed of Ag, Se, Fe, Co, Cu, and S. Analysis suggests that these metals were derived from dental amalgam and that the discoloration was caused by amalgam tattoo.
Enhancing Effects of Fluorescein on β‐Lactam Rash II: Enhancing Effects of Fluorescein on Generalized Rash Induced by β‐Lactam Antibiotics in Guinea Pigs
Authors: Ikezawa Zenro , Sugihara Yoshiki , Ueno Junko 
Published: April 2015

AbstractHealthy volunteers, who were receiving intravenous injections of cefclidin (CFCL) with frequent concomitant use of fluorescein (F) and oxybuprocain (O) in the eyes for measurement of ocular tension, developed drug eruptions at the high frequency of 66.7%. The injection of CFCL alone induced the eruptions at an incidence of 2.8%. The cause of this high eruption rate was thought to be the simultaneous treatment with F and/or O. Therefore, we conducted experiments with CFCL‐induced generalized rash (GR) in guinea pigs. Guinea pigs treated with F and O during both the phases of immunization and intraperitoneal elicitation developed CFCL rashes at a high frequency. This CFCL‐rash was augmented by the treatment with F during either phase, but not by the treatment with O. Skin testing induced delayed type hypersensitivity (DTH) reaction to O in some animals, but the DTH to F was not induced in animals immunized with F in complete Freund's adjuvant. Furthermore, F augmented rashes induced not only by CFCL but also by other β‐lactam antibiotics such as cefsulodin and sulbenicillin.Accordingly, it is likely that F played a dominant role in the high incidence of drug eruptions during the volunteer trials with measurement of ocular tension.
Aneuploidy in Chronic Actinic Dermatitis
Authors: Bingsen Qiu , Kanghuang Liao , Hongzhen Ni 
Published: April 2015

AbstractDNA content and cell cycle distributions in paraffin‐embedded blocks of 19 skin biopsy specimens from 17 patients with chronic actinic dermatitis (CAD) [8 patients showed typical actinic reticuloid (AR)] were estimated by DNA cytometry. Sixty‐three percent (12/19) of the skin specimens showed aneuploidy. In the 4 cases with the highest DNA indices (DI), ranging from 1.65 to 1.88 (mean: 1.84), the proportions of cells in S and G2/M phases were increased, ranging from 15–48% (mean: 20%) and from 64–76% (mean: 70.5%), respectively. In 8 cases with DI ranging from 1.15–1.75 (mean: 1.5), the proportion of cells in S‐phase was also increased, ranging from 30–90% (mean: 81.1%). Histologically, it seems likely that the relatively high frequency of aneuploidy, DI, and proportions of cells in the S and/or G2/M phases were not proportional to epidermal or vascular endothelial hyperplasia, but they might be related to dermal lymphoid infiltration.
Angiokeratoma of the Clitoris: A Subtype of Angiokeratoma Vulvae
Authors: Yamazaki Masashi , Hiruma Masataro , Irie Hiroya , Ishibashi Akira 
Published: April 2015

AbstractA 24‐year‐old married woman had an angiokeratoma of the vulva measuring 3 × 3 × 4 mm localized at the clitoris. The nodule was noticed when she was pregnant with her first child. This tumor grew with occasional tenderness and bleeding during pregnancy, but its size did not change after delivery. The growth of this tumor might be attributable to an increase in venous pressure or serum progesterone levels; therefore this disorder might be classified as a subtype of angiokeratoma of the vulva. This is the first case of angiokeratoma localized at the clitoris.
Expression of CA 50 in Normal Human Skin: Comparative Study of CEA and CA 19‐9
Authors: Inaba Youichi , Egawa Kiyofumi , Kageshita Toshiro , Ono Tomomichi 
Published: April 2015

AbstractNormal skin specimens from different body sites were estimated for the expression of CA 50 in comparison with CA 19‐9 and CEA. Expression of CA 50 and CA 19‐9 was observed in the ductal system and a small part of the secretory portion of eccrine units, but never in any other skin components including apocrine units. CEA was expressed on the ductal and a small part of the secretory portion of apocrine units as well as on those of eccrine units. However, the expression pattern and intensity in eccrine units were different from those of CA 50 or CA 19‐9.We concluded that CA 50 was strongly expressed in the normal ductal system of eccrine units and hence could be standardized to stain the eccrine ductal portion and that immunohistochemical comparative studies with CA 19‐9 or CEA might be a good methodological technique for discriminating an eccrine or apocrine origin for some skin disorders.
Localized Basaloid Follicular Hamartoma
Authors: Kato Naoko , Ueno Hiroo , Nakamura Junnosuke 
Published: April 2015

AbstractWe report a 39‐year‐old Japanese woman with a 30 × 10 mm, skin‐colored, soft, hairless, irregularly elevated plaque in the left retroauricular area. Histologically, it consistently showed features of atypical hair follicles; some showed immaturely developed basaloid epithelial islands invaginated with condensed stromal cells, and others were replaced or associated with solid strands, branching cords, or lace‐like networks of undifferentiated basaloid cells. Although the clinical appearance of a typical localized basaloid follicular hamartoma is a plaque of alopecia, this case seems to be a variant, because the histological findings are so characteristic of the entity. The relationship of basaloid follicular hamartoma to other hair‐follicle derived tumors is also considered.
Structure, Function and Possible Clinical Application of Transforming Growth Factor‐β
Authors: Miyazono Kohei , Heldin Carl‐Henrik 
Published: April 2015

AbstractTransforming growth factor‐β (TGF‐β) is a family of multifunctional 25 kDa proteins. TGF‐β was originally identified because of its ability to induce the growth of normal rodent fibroblasts in soft agar, but is now known as a potent growth inhibitor for many different cell types. In addition, TGF‐β is known to regulate the differentiation of cells, induce chemotaxis of cells, and to induce the accumulation of extracellular matrix proteins. In vivo, TGF‐β stimulates the repair of soft as well as hard tissues. It also acts as a potent immunosuppressant.TGF‐β is produced as latent high molecular weight complexes; since it is produced by many different cell types, and most cells have receptors for TGF‐β, the activation of latent TGF‐β is likely to be an important step in the regulation of its action. TGF‐β exerts its effects by binding to specific cell surface receptors. The type I and type II TGF‐β receptors are suggested to be the most important for signal transduction; a recent report has disclosed that the type II receptor has a serine/threonine kinase domain.Since TGF‐β is a potent growth regulator with multifunctional activity, it may be useful in the treatment of certain clinical disorders. Local application of TGF‐β is shown to accelerate wound healing. Since an increase in TGF‐β activity is often observed in various fibrotic disorders, antagonists for TGF‐β might be valuable in the treatment of such diseases.
Stimulation of Keratinocyte Migration by Growth Factors
Authors: Tsuboi Ryoji , Sato Chiyo , Shi Chong‐Ming , Ogawa Hideoki 
Published: April 2015

AbstractMigration of keratinocytes from the wound edge is thought to be one of the critical features of reepithelialization. A quantitative migration assay was carried out using normal human keratinocytes. Keratinocytes, seeded on 12 well plates, were grown in serum free, keratinocyte growth medium (KGM, Curabo Co) with 0.08mM Ca++. The medium was switched from KGM to keratinocyte basal medium (KBM) 6 h prior to the wounding. Half of the plate's confluent monolayer of keratinocytes was removed with a razor blade, and the remaining keratinocytes were incubated in KBM for 16 hrs in the presence of indicated growth factors. After incubation, the cells were fixed and counted at 100 magnification. Migration was quantitated by counting the number of cells in ten successive 125‐μm zones.Transforming growth factor α (TGF‐α), acidic and basic fibroblast growth factor (aFGF, bFGF), keratinocyte growth factor (KGF), hepatocyte growth factor (HGF), and insulin‐like growth factor‐I (IGF‐I) stimulated the migration of keratinocytes, while TGF‐β suppressed it.
The Effects of Basic Fibroblast Growth Factor and Doxorubicin on Cultured Human Skin Fibroblasts: Relevance to Wound Healing
Authors: Sasaki Tetsuo 
Published: April 2015

AbstractThe effects of basic fibroblast growth factor (bFGF) and doxorubicin on cultured human skin fibroblasts were examined in order to determine their relevance to wound healing. bFGF is shown to stimulate fibroblast collagenase production per cell, and this effect in vitro seems to be one explanation for its efficacy in wound healing. Doxorubicin inhibited not only fibroblast proliferation but also collagen production by inactivating prolyl hydroxylase. This result may explain the reduced wound healing in patients undergoing treatment with doxorubicin. These studies indicate the importance of assessing the effects of growth factors on matrix metabolism in order to understand their roles in wound healing.
A Wound Healing Model Using Healing‐impaired Diabetic Mice
Authors: Tsuboi Ryoji , Shi Chong‐Ming , Rifkin Daniel B. , Ogawa Hideoki 
Published: April 2015

AbstractA quantitative histological approach was employed to evaluate the effects of basic fibroblast growth factor (bFGF) in healing‐impaired diabetic mice. The dorsal areas of female mutant diabetic mice, C57BL KsJ db/db (Jackson Lab.), were given two 6 mm‐size full thickness wounds with a punch biopsy instrument. After application of bFGF, the wounds were left open. 8 days after wounding, the mice were sacrificed, and histological sections were evaluated using several histological parameters, such as the degree of wound closure, granulation tissue thickness, matrix density, and capillary numbers.Application of 5 μg of bFGF for 5 days induced significant responses by all of these dermal parameters when compared to those of non‐treated db/db mice (p<0.001). A minimum of 0.5 μg bFGF per day was required for a significant effect. Time‐course experiments indicated that the granulation response in bFGF‐treated mice peaked between 8 and 12 days and decreased after 12 days, while matrix density continued to increase until the 18th day.
Alterations of Mitogenic Responses of Mononuclear Cells by Arsenic in Arsenical Skin Cancers
Authors: Yu Hsin‐Su , Chang Kee‐Lung , Wang Ching‐Ming , Yu Chia‐Li 
Published: April 2015

AbstractWe have studied the endemic occurrence of chronic arsenism in a limited area on the southwest coast of Taiwan. The effects of arsenic on the mitogenic responses of mononuclear cells (MNC) derived from patients with arsenical skin cancers in that area were evaluated. The subjects enrolled in this study included patients with 1) Bowen's disease, 2) arsenical skin cancers (basal cell carcinoma and squamous cell carcinoma), 3) non‐arsenical skin cancers (basal cell carcinoma and squamous cell carcinoma), 4) nasopharyngeal cancer and 5) healthy controls from endemic and non‐endemic areas. Phytohemagglutinin (PHA) stimulated [3H]thymidine incorporation in MNC in all groups except the arsenical skin cancer group. However, when a low concentration of As2O3 (2.5 × 10−7 M) was added to PHA‐stimulated MNC, a tremendous amplification of the uptake of [3H]thymidine was noticed in patients with arsenical skin cancer. In this study, this phenomenon did not occur in cancers not related to arsenic. This result shows that arsenical carcinomas are hyperreactive to its specific etiology—arsenic. Arsenic seems to play a role as a co‐stimulant of PHA similar to interleukin‐1.