bluuebunny/crossref_metadata_2025_split huggingface dataset:
Entorhinal cortex: a good biomarker of mild cognitive impairment and mild Alzheimer’s disease
Authors: Zhou Mengxi , Zhang Feng , Zhao Li , Qian Jin , Dong Chunbo 
Published: October 2015

Abstract
Entorhinal cortex (EC), thought to be the location of the earliest lesions in Alzheimer’s disease (AD), has been widely studied in recent years. With the irreversible pathological changes of AD, there is an urgent need to find biomarkers that can be used to predict the presence of the disease before it is clinically expressed. The aim of this review is to summarize and analyze recent findings that are relevant to the important role of EC in the diagnosis of mild cognitive impairment (MCI) and mild AD and to describe a range of neuroimaging techniques used to define the EC boundary. A comprehensive literature search for articles published up to May 2015 was performed. Our research highlights the finding that atrophy in EC reflects the early pathological changes of AD and can be a strong predictor of prodromal AD. The early changes in EC are a good imaging biomarker that can be used to discriminate individuals with MCI from normal control subjects. A larger degree of atrophy in EC predicts increased disease severity, and the right EC in patients with mild AD exhibited greater changes than the left side. In addition, the EC seems to have an obvious advantage over the hippocampus as a biomarker when predicting future conversion to AD in individuals with MCI, and it may be of help in following the course of disease progression. In this review, we also summarize the main differences observed between the hippocampus and the EC when differentiating diseases. These findings will hopefully provide an opportunity for the effective prevention and early treatment of AD.
The bridge between two worlds: psychoanalysis and fMRI
Authors: Marini Stefano , Di Tizio Laura , Dezi Sira , Armuzzi Silvia , Pelaccia Simona , Valchera Alessandro , Sepede Gianna , Girinelli Gabriella , De Berardis Domenico , Martinotti Giovanni , Gambi Francesco , Di Giannantonio Massimo 
Published: October 2015

AbstractIn recent years, a connection between psychoanalysis and neuroscience has been sought. The meeting point between these two branches is represented by neuropsychoanalysis. The goal of the relationship between psychoanalysis and neuroscience is to test psychoanalytic hypotheses in the human brain, using a scientific method. A literature search was conducted on May 2015. PubMed and Scopus databases were used to find studies for the inclusion in the systematic review. Common results of the studies investigated are represented by a reduction, a modulation, or a normalization of the activation patterns found after the psychoanalytic therapy. New findings in the possible and useful relationship between psychoanalysis and neuroscience could change the modalities of relating to patients for psychoanalysts and the way in which neuroscientists plan their research. Researchers should keep in mind that in any scientific research that has to do with people, neuroscience and a scientific method cannot avoid subjective interpretation.
Hydraulic Circuit of Mechanical Pruner Drive for Hops on Low Trellises
Authors: Hoffmann David , Heřmánek P. , Rybka A. , Jošt B. 
Published: October 2015

Abstract
A mechanical pruner serves for pruning new hopvine shoots in spring. The later yield depends on the right timing and quality of pruning. That is why hop pruning is one of the most important agrotechnical procedures. A double-disc mechanical pruner used on high trellises cannot be used on low trellises due to its large size. Abroad, for pruning hops on low trellises a specially adapted sprinkler is used (chemical pruning). With regard to the effort to minimize the chemical environmental burden, we opted for the design of the mechanical pruner. Firstly, the low trellis, mechanical pruner, and also elements used in the design of hydraulic circuit are described. Next part of the paper is devoted to the input requirements for both the hydraulic circuit and the mechanical pruner designs. Then a description of an adapted inter-axle carrier used for the experimental model of the hop mechanical pruner and of the effected field measurement follows, along with interpretation of the measured data. These data are depicted in clearly arranged graphs showing the dependency of pressure and hydraulic oil flow on the cutting disc rotational frequency.
Differences in the Financial Management of Conventional, Organic, and Biodynamic Farms
Authors: Vlašicová Eliška , Náglová Z. 
Published: October 2015

Abstract
The financial management of conventional, organic, and biodynamic farms was evaluated and compared. It is a highly specific issue filling in the gap namely in the area of economic research of biodynamic agriculture. Biodynamic agriculture is a less widespread concept of agriculture, the management of which meets the requirements of organic agriculture. Organic agriculture has still been gaining in importance in the Czech Republic, the number of organic farms has been growing, and availability of organic products has increased, too. Of the Czech farms receiving subsidies from the EU or state subsidies in 2007-2012, a total of 389 were selected for analysis (273 of which were conventional farms, 112 organic farms, and 4 farms were engaged in biodynamic agriculture). Subsidies, Total Costs, Operating Revenue, Profit and Gross Value Added indicators were selected for evaluation. The individual indicators within groups of companies were compared by means of a t-test. The analysis revealed significant differences in the economic indicators of individual types of farms. It was observed that organic enterprises have better economic results than conventional and biodynamic businesses. Subsidies help all types of farms achieve better results. We may hence assume dependence of these farms on subsidies.
Investigation of the ν
2 + 2ν
3 Subband in the Overtone Icosad of 13CH4 by Pulsed Supersonic Jet and Diode Laser Cavity Ring-Down Spectroscopy: Partial Rovibrational Analysis and Nuclear Spin Symmetry Conservation
Authors: Bjelobrk Zoran , Manca Tanner Carine , Quack Martin 
Published: October 2015

Abstract
We have investigated the ν
2 + 2ν
3 combination band of methane 13CH4 centered at
7493.15918 cm
–1 within the icosad of the overtone absorption. The jet-CRD setup combining supersonic jet
expansions and cavity ring-down spectroscopy which was already used for the reinvestigation of the same spectral region for the
main isotope of methane (12CH4) has been used to record spectra of the Q and R branches at room temperature as
well as at very low temperatures (down to 4 K). Based on our previous temperature-dependent investigations and the
present results, we provide a careful analysis and the assignment for lines involving angular momentum quantum numbers up to
J = 4. The analysis of the relative intensities in spectra taken at various rotational and effective translational temperatures
demonstrate conservation of nuclear spin symmetry for 13CH4 under the conditions of a supersonic jet expansion,
similar to our previous results regarding 12CH4 and also to further results using other techniques and covering
other spectral ranges. This is in agreement with theoretical expectation regarding very slow nuclear spin symmetry relaxation
under these conditions in supersonic jet expansions.
Cortical Low-Frequency Power and Progressive Phase Synchrony Precede Successful Memory Encoding
Authors: Haque Rafi U. , Wittig John H. , Damera Srikanth R. , Inati Sara K. , Zaghloul Kareem A. 
Published: October 2015

Neural activity preceding an event can influence subsequent memory formation, yet the precise cortical dynamics underlying this activity and the associated cognitive states remain unknown. We investigate these questions here by examining intracranial EEG recordings as 28 participants with electrodes placed for seizure monitoring participated in a verbal paired-associates memory task. We found that, preceding successfully remembered word pairs, an orientation cue triggered a low-frequency 2–4 Hz phase reset in the right temporoparietal junction with concurrent increases in low-frequency power across cortical regions that included the prefrontal cortex and left temporal lobe. Regions that exhibited a significant increase in 2–4 Hz power were functionally bound together through progressive low-frequency 2–4 Hz phase synchrony. Our data suggest that the interaction between power and phase synchrony reflects the engagement of attentional networks that in large part determine the extent to which memories are successfully encoded.SIGNIFICANCE STATEMENTHere we investigate the spatiotemporal cortical dynamics that precede successful memory encoding. Using intracranial EEG, we observed significant changes in oscillatory power, intertrial phase consistency, and pairwise phase synchrony that predict successful encoding. Our data suggest that the interaction between power and phase synchrony reflects the engagement of attentional networks that in large part determine the extent to which memories are successfully encoded.
TASK Channels on Basal Forebrain Cholinergic Neurons Modulate Electrocortical Signatures of Arousal by Histamine
Authors: Vu Michael T. , Du Guizhi , Bayliss Douglas A. , Horner Richard L. 
Published: October 2015

Basal forebrain cholinergic neurons are the main source of cortical acetylcholine, and their activation by histamine elicits cortical arousal. TWIK-like acid-sensitive K+(TASK) channels modulate neuronal excitability and are expressed on basal forebrain cholinergic neurons, but the role of TASK channels in the histamine-basal forebrain cholinergic arousal circuit is unknown. We first expressed TASK channel subunits and histamine Type 1 receptors in HEK cells. Application of histaminein vitroinhibited the acid-sensitive K+current, indicating a functionally coupled signaling mechanism. We then studied the role of TASK channels in modulating electrocortical activityin vivousing freely behaving wild-type (n= 12) and ChAT-Cre:TASKf/fmice (n= 12), the latter lacking TASK-1/3 channels on cholinergic neurons. TASK channel deletion on cholinergic neurons significantly altered endogenous electroencephalogram oscillations in multiple frequency bands. We then identified the effect of TASK channel deletion during microperfusion of histamine into the basal forebrain. In non-rapid eye movement sleep, TASK channel deletion on cholinergic neurons significantly attenuated the histamine-induced increase in 30–50 Hz activity, consistent with TASK channels contributing to histamine action on basal forebrain cholinergic neurons. In contrast, during active wakefulness, histamine significantly increased 30–50 Hz activity in ChAT-Cre:TASKf/fmice but not wild-type mice, showing that the histamine response depended upon the prevailing cortical arousal state. In summary, we identify TASK channel modulation in response to histamine receptor activationin vitro, as well as a role of TASK channels on cholinergic neurons in modulating endogenous oscillations in the electroencephalogram and the electrocortical response to histamine at the basal forebrainin vivo.SIGNIFICANCE STATEMENTAttentive states and cognitive function are associated with the generation of γ EEG activity. Basal forebrain cholinergic neurons are important modulators of cortical arousal and γ activity, and in this study we investigated the mechanism by which these neurons are activated by the wake-active neurotransmitter histamine. We found that histamine inhibited a class of K+leak channels called TASK channels and that deletion of TASK channels selectively on cholinergic neurons modulated baseline EEG activity as well as histamine-induced changes in γ activity. By identifying a discrete brain circuit where TASK channels can influence γ activity, these results represent new knowledge that enhances our understanding of how subcortical arousal systems may contribute to the generation of attentive states.
Reach Trajectories Characterize Tactile Localization for Sensorimotor Decision Making
Authors: Brandes Janina , Heed Tobias 
Published: October 2015

Spatial target information for movement planning appears to be coded in a gaze-centered reference frame. In touch, however, location is initially coded with reference to the skin. Therefore, the tactile spatial location must be derived by integrating skin location and posture. It has been suggested that this recoding is impaired when the limb is placed in the opposite hemispace, for example, by limb crossing. Here, human participants reached toward visual and tactile targets located at uncrossed and crossed feet in a sensorimotor decision task. We characterized stimulus recoding by analyzing the timing and spatial profile of hand reaches. For tactile targets at crossed feet, skin-based information implicates the incorrect side, and only recoded information points to the correct location. Participants initiated straight reaches and redirected the hand toward a target presented in midflight. Trajectories to visual targets were unaffected by foot crossing. In contrast, trajectories to tactile targets were redirected later with crossed than uncrossed feet. Reaches to crossed feet usually continued straight until they were directed toward the correct tactile target and were not biased toward the skin-based target location. Occasional, far deflections toward the incorrect target were most likely when this target was implicated by trial history. These results are inconsistent with the suggestion that spatial transformations in touch are impaired by limb crossing, but are consistent with tactile location being recoded rapidly and efficiently, followed by integration of skin-based and external information to specify the reach target. This process may be implemented in a bounded integrator framework.SIGNIFICANCE STATEMENTHow do you touch yourself, for instance, to scratch an itch? The place you need to reach is defined by a sensation on the skin, but our bodies are flexible, so this skin location could be anywhere in 3D space. The movement toward the tactile sensation must therefore be specified by merging skin location and body posture. By investigating human hand reach trajectories toward tactile stimuli on the feet, we provide experimental evidence that this transformation process is quick and efficient, and that its output is integrated with the original skin location in a fashion consistent with bounded integrator decision-making frameworks.
Fast Oscillatory Commands from the Motor Cortex Can Be Decoded by the Spinal Cord for Force Control
Authors: Watanabe Renato N. , Kohn Andre F. 
Published: October 2015

Oscillations in the beta and gamma bands (13–30 Hz; 35–70 Hz) have often been observed in motor cortical outputs that reach the spinal cord, acting on motoneurons and interneurons. However, the frequencies of these oscillations are above the muscle force frequency range. A current view is that the transformation of the motoneuron pool inputs into force is linear. For this reason possible roles for these oscillations are unclear, since if this transformation is linear, the high frequencies in the motoneuron inputs (e.g., 20 Hz from pyramidal tract neurons) would be filtered out by the muscle and have no effect on force control. A biologically inspired mathematical model of the neuromuscular system was used to investigate the impact of high-frequency cortical oscillatory activity on force control. The model simulation results evidenced that a typical motoneuron pool has a nonlinear behavior that enables the decoding of a high-frequency oscillatory input. An input at a single frequency (e.g., beta band) leads to an increase in the steady-state force generated by the muscle. When the input oscillation was amplitude modulated at a given low frequency, the force oscillated at this frequency. In both cases, the mechanism relies on the recruitment and derecruitment of motor units in response to the oscillatory descending drive. Therefore, the results from this study suggest a potential role in force control for cortical oscillations at frequencies at or above the beta band, despite the low-pass behavior of the muscles.SIGNIFICANCE STATEMENTThe role of cortical oscillations in motor control has been a long-standing question, one view being that they are an epiphenomenon. Fast oscillations are known to reach the spinal cord, and hence they have been thought to affect muscle behavior. However, experimental limitations have hampered further advances to explain how they could influence muscle force. An approach for such a challenge was adopted in the present research: to study the problem through computer simulations of an advanced biologically compatible mathematical model. Using such a model, we found that the well-known mechanism of recruitment and derecruitment of the spinal cord motoneurons can allow the muscle to respond to cortical oscillations, suggesting that these oscillations are not epiphenomena in motor control.
MaternalUbe3aLoss Disrupts Sleep Homeostasis But Leaves Circadian Rhythmicity Largely Intact
Authors: Ehlen J. Christopher , Jones Kelly A. , Pinckney Lennisha , Gray Cloe L. , Burette Susan , Weinberg Richard J. , Evans Jennifer A. , Brager Allison J. , Zylka Mark J. , Paul Ketema N. , Philpot Benjamin D. , DeBruyne Jason P. 
Published: October 2015

Individuals with Angelman syndrome (AS) suffer sleep disturbances that severely impair quality of life. Whether these disturbances arise from sleep or circadian clock dysfunction is currently unknown. Here, we explored the mechanistic basis for these sleep disorders in a mouse model of Angelman syndrome (Ube3am−/p+mice). Genetic deletion of the maternalUbe3aallele practically eliminates UBE3A protein from the brain ofUbe3am−/p+mice, because the paternal allele is epigenetically silenced in most neurons. However, we found that UBE3A protein was present in many neurons of the suprachiasmatic nucleus—the site of the mammalian circadian clock—indicating thatUbe3acan be expressed from both parental alleles in this brain region in adult mice. We found that whileUbe3am−/p+mice maintained relatively normal circadian rhythms of behavior and light-resetting, these mice exhibited consolidated locomotor activity and skipped the timed rest period (siesta) present in wild-type (Ube3am+/p+) mice. Electroencephalographic analysis revealed that alterations in sleep regulation were responsible for these overt changes in activity. Specifically,Ube3am−/p+mice have a markedly reduced capacity to accumulate sleep pressure, both during their active period and in response to forced sleep deprivation. Thus, our data indicate that the siesta is governed by sleep pressure, and thatUbe3ais an important regulator of sleep homeostasis. These preclinical findings suggest that therapeutic interventions that target mechanisms of sleep homeostasis may improve sleep quality in individuals with AS.SIGNIFICANCE STATEMENTAngelman syndrome (AS) is a severe neurodevelopmental disorder caused by loss of expression of the maternal copy of theUBE3Agene. Individuals with AS have severe sleep dysfunction that affects their cognition and presents challenges to their caregivers. Unfortunately, current treatment strategies have limited efficacy due to a poor understanding of the mechanisms underlying sleep disruptions in AS. Here we demonstrate that abnormal sleep patterns arise from a deficit in accumulation of sleep drive, uncovering theUbe3agene as a novel genetic regulator of sleep homeostasis. Our findings encourage a re-evaluation of current treatment strategies for sleep dysfunction in AS, and suggest that interventions that promote increased sleep drive may alleviate sleep disturbances in individuals with AS.
Synaptic Connectivity between Renshaw Cells and Motoneurons in the Recurrent Inhibitory Circuit of the Spinal Cord
Authors: Moore Niall J. , Bhumbra Gardave S. , Foster Joshua D. , Beato Marco 
Published: October 2015

Renshaw cells represent a fundamental component of one of the first discovered neuronal circuits, but their function in motor control has not been established. They are the only central neurons that receive collateral projections from motor outputs, yet the efficacy of the excitatory synapses from single and converging motoneurons remains unknown. Here we present the results of dual whole-cell recordings from identified, synaptically connected Renshaw cell-motoneuron pairs in the mouse lumbar spinal cord. The responses from single Renshaw cells demonstrate that motoneuron synapses elicit large excitatory conductances with few or no failures. We show that the strong excitatory input from motoneurons results from a high probability of neurotransmitter release onto multiple postsynaptic contacts. Dual current-clamp recordings confirm that single motoneuron inputs were sufficient to depolarize the Renshaw cell beyond threshold for firing. Reciprocal connectivity was observed in approximately one-third of the paired recordings tested. Ventral root stimulation was used to evoke currents from Renshaw cells or motoneurons to characterize responses of single neurons to the activation of their corresponding presynaptic cell populations. Excitatory or inhibitory synaptic inputs in the recurrent inhibitory loop induced substantial effects on the excitability of respective postsynaptic cells. Quantal analysis estimates showed a large number of converging inputs from presynaptic motoneuron and Renshaw cell populations. The combination of considerable synaptic efficacy and extensive connectivity within the recurrent circuitry indicates a role of Renshaw cells in modulating motor outputs that may be considerably more important than has been previously supposed.SIGNIFICANCE STATEMENTWe have recently shown that Renshaw cells mediate powerful shunt inhibition on motoneuron excitability. Here we complete a quantitative description of the recurrent circuit using recordings of excitatory synapses between identified motoneuron and Renshaw cell pairs. We show that the excitation is highly effective as a result of a high probability of neurotransmitter release onto multiple release sites and that efficient neurotransmission is maintained at physiologically relevant firing rates in motoneurons. Our results also show that both excitatory and inhibitory connections exhibit considerable convergence of inputs. Because evaluation of the determinants of synaptic strength and the extent of connectivity constitute fundamental parameters affecting the operation of the recurrent circuit, our findings are critical for informing any future models of motor control.