bluuebunny/crossref_metadata_2025_split huggingface dataset:
A study of bacteria producing carbonic anhydrase enzyme for CaCO3 precipitation and soil biocementation
Authors: Mwandira Wilson , Mavroulidou Maria , Timmermans Martijn , Gunn Michael John , Gray Christopher , Pantoja-Muñoz Leonardo , Purchase Diane 
Published: July 2024

AbstractWe study the carbonic anhydrase (CA) pathway using autochthonous CA-producing bacteria as a means of inducing calcite precipitation, which acts as a biocement to improve the engineering soil properties. Forty different microbial strains producing CA were isolated from the foundation soil of a railway embankment in Prickwillow, UK. Three of the best CA-producing strains were selected and identified by DNA sequencing as Bacillus licheniformis, Bacillus toyonensis and Bacillus pumilus with CA activity values respectively of 1.79 U/ml, 1.42 U/ml and 1.55 U/ml. To optimise the treatments, we investigated the effect of pH, temperature, zinc co-factor and cementation solution molarity on the growth and CA activity and bioprecipitates, with CO2 added in the form of bicarbonate. Scanning electron microscope (SEM) analysis of the bioprecipitates showed that these had characteristic morphologies of calcite and vaterite crystals. The formation of calcite was further corroborated by FT-IR and Raman analysis of bioprecipitates. The precultured bacteria were injected into the fine-grained soil together with cementation solution. Unconfined compressive strength in treated soil increased up to 1 MPa, and its calcium carbonate content increased by 2.78%. This, as well as the stability of the treated soil upon water immersion, proved the biocementation of the fine-grained soil. These findings suggest the potential of employing the CA biocementation route for soil stabilisation pending further development of the technique.
Long-term impact of basin-wide wastewater management on faecal pollution levels along the entire Danube River
Authors: Kirschner Alexander K. T. , Schachner-Groehs Iris , Kavka Gerhard , Hoedl Edith , Kovacs Adam , Farnleitner Andreas H. 
Published: July 2024

AbstractThe Danube River is, at 2857 km, the second longest river in Europe and the most international river in the world with 19 countries in its catchment. Along the entire river, faecal pollution levels are mainly influenced by point-source emissions from treated and untreated sewage of municipal origin under base-flow conditions. In the past 2 decades, large investments in wastewater collection and treatment infrastructure were made in the European Union (EU) Member States located in the Danube River Basin (DRB). Overall, the share of population equivalents with appropriately biologically treated wastewater (without disinfection) has increased from 69% to more than 85%. The proportion of tertiary treatment has risen from 46 to 73%. In contrast, no comparable improvements of wastewater infrastructure took place in non-EU Member States in the middle and lower DRB, where a substantial amount of untreated wastewater is still directly discharged into the Danube River. Faecal pollution levels along the whole Danube River and the confluence sites of the most important tributaries were monitored during four Danube River expeditions, the Joint Danube Surveys (JDS). During all four surveys, the longitudinal patterns of faecal pollution were highly consistent, with generally lower levels in the upper section and elevated levels and major hotspots in the middle and lower sections of the Danube River. From 2001 to 2019, a significant decrease in faecal pollution levels could be observed in all three sections with average reduction rates between 72 and 86%. Despite this general improvement in microbiological water quality, no such decreases were observed for the highly polluted stretch in Central Serbia. Further improvements in microbiological water quality can be expected for the next decades on the basis of further investments in wastewater infrastructure in the EU Member States, in the middle and lower DRB. In the upper DRB, and due to the high compliance level as regards collection and treatment, improvements can further be achieved by upgrading sewage treatment plants with quaternary treatment steps as well as by preventing combined sewer overflows. The accession of the Western Balkan countries to the EU would also significantly boost investments in wastewater infrastructure and water quality improvements in the middle section of the Danube. Continuing whole-river expeditions such as the Joint Danube Surveys is highly recommended to monitor the developments in water quality in the future.
Conversion of rice husks into carbonaceous materials with porous structures via hydrothermal process
Authors: Sugie Sayaka , Maeda Hirotaka 
Published: July 2024

AbstractCarbonaceous materials hydrothermally produced using waste biomass have small specific surface areas (SSA) and poor porosity properties. In this study, we prepare a novel carbonaceous material with excellent porosity properties by suppressing the formation of a secondary char phase (spheres) and promoting biomass hydrolysis by controlling the hydrothermal conditions. Rice husk powders, as the starting material, are hydrothermally treated using acidic solvents of different types and concentrations at 180 °C. The surfaces of the samples hydrothermally prepared using the acidic solvents have no spheres. In the case of 0.1–0.2 mol L−1 hydrochloric acid (HA), the amorphous carbonaceous materials contain numerous mesopores and exhibit a larger SSA (approximately 100 m2 g−1) than those prepared using acetic acid and distilled water. An increase in the hydrothermal temperature reduces the porosity properties of the materials. Finally, the high-porosity amorphous carbonaceous material showed excellent trimethylamine adsorption ability.
Enhancing middle school students’ computational thinking competency through game-based learning
Authors: Pan Yanjun , Adams Elizabeth L. , Ketterlin-Geller Leanne R. , Larson Eric C. , Clark Corey 
Published: July 2024

AbstractComputational thinking is acknowledged as an essential competency for everyone to learn. However, teachers find it challenging to implement the existing learning approaches in K-12 settings because the existing approaches often focus on teaching computing concepts and skills (i.e., programming skills) rather than on helping students develop their computational thinking competency—a competency that can be used across disciplinary boundaries in accordance with curriculum requirements. To address this need, the current study investigated how game-based learning influenced middle school students’ learning processes, particularly on the development of computational thinking competency, self-efficacy toward computational thinking, and engagement during gameplay. Additionally, the study examined how these outcomes were moderated by individual differences. We observed evidence that the gaming experience influenced students’ computational thinking self-efficacy, but not computational thinking competency or game-based engagement. Compared to age (grade) and prior gaming experience, gender tended to play a more important role in moderating students’ computational thinking competency, self-efficacy toward computational thinking competency, and game-based engagement. Implications and possible directions for future research regarding using game-based learning to enhance computational thinking competency are discussed.
Effect of Microstructure on Oxidation and Micro-mechanical Behavior of Arc Consolidated Mo-Ti-Si-(B) Alloys
Authors: Paul Bhaskar , Kumar Shubham , Kishor J. , Majumdar Sanjib 
Published: July 2024

AbstractThe present study deals with the development and characterization of Mo-35Ti-10Si and Mo-35Ti-10Si-2B (wt.%) alloy for ultra-high temperature applications beyond the temperature limit of existing super alloys. The microstructural characterization using scanning electron microscopy (SEM), energy dispersive spectrometry (EDS), electron back scattered diffraction (EBSD), x-ray diffraction (XRD) revealed that the Mo-35Ti-10Si-2B alloy was consisted of three phases, namely, (Mo, Ti)ss, (Mo, Ti)5SiB2 and (Ti, Mo)5Si3; whereas, Mo-35Ti-10Si alloy was found to be consisting of (Mo, Ti)ss, and (Mo,Ti)3Si phases. Since quantification of boron is difficult by EDS, Particle Induced Gamma-ray Emission (PIGE), a nuclear reaction analysis technique was used for chemical composition analysis of boron. The oxidation behavior of the Mo-35Ti-10Si-2B alloy in the temperature regime of 825-1250 °C was studied in detail and compared with boron-free Mo-35Ti-10Si alloy. Mo-35Ti-10Si-2B alloy exhibited superior oxidation behavior at intermediate temperatures of 825 °C, and excellent oxidation resistance at higher temperatures between 1000 and 1250 °C due to the formation of the protective borosilica and double oxide layers (TiO2 and duplex borosilica-TiO2), respectively. High-temperature oxidation mechanisms were discussed using detailed microstructural cross section analysis of the oxidized alloy samples. The micro-mechanical behavior of constitutive phases of the Mo-35Ti-10Si-2B alloy were studied by microhardness, nano-indentation and micropillar compression testing. The micropillar compression of (Mo, Ti)ss phase showed fairly ductile behavior with the evidence of activation of dislocation in the form of slip lines revealed through the post-deformation fractography. Deformation studies of (Mo, Ti)5SiB2 and (Ti, Mo)5Si3 phases were also carried out which showed large strain bursts indicating possibility of activation of dislocation activities even at room temperatures imparting low level of ductility.
Debonding of Porous Coating: A Late Failure Mode of Uncemented, Partially Threaded Acetabular Components—Retrieval Analysis
Authors: Łapaj Łukasz , Sulej-Chojnacka Joanna , Rozwalka Justyna , Alaouir Abdulrahim , Markiewicz Tomasz , Chodór Paweł , Kiryluk Jan , Mróz Adrian , Zabrzyński Jan 
Published: July 2024

AbstractTitanium plasma-sprayed (TPS) porous coatings have been used in total hip arthroplasty for decades. They are considered reliable, and very few failure cases have been described so far. This retrieval study described a series of 20 acetabular components—where total or partial debonding occurred during in vivo use and aimed to explain the underlying failure mechanisms. Implants were examined using optical and electron microscopy (SEM), metallographic sections of retrievals were prepared while pathologic samples of periprosthetic tissues were examined for presence of wear debris. Data from metallographic slides indicated that debonding was initiated at free borders of the coating and tended to progress at the interface between the TPS layer and the shell. In some cases, total debonding occurred leading material wear of both the TPS layer and acetabular shell leading to massive release of metallic debris and accelerated polyethylene wear in third body mechanism. SEM examination demonstrated that splats forming the TPS layer exhibited features suggesting a high temperature gradient between the plasma sprayed layer and the substrate material existed, leading to porosity of splats and suboptimal bonding strength. This study demonstrated that coating application parameters and certain design features (screw holes, fins) may promote long-term failure due to debonding. Surgeons should be aware of this complication as it is most likely underreported, while manufacturers should consider more rigorous pre-clinical testing as suboptimal coating bonding may result in failures during long-term clinical use.
The contribution of E3D imaging integrated with robotic navigation: analysis of the first 80 consecutive posterior spinal fusion cases
Authors: Stewart Jeffrey J. , Asdourian Paul , Moatz Bradley , Soda Mosope , Lemma Mesfin , Cunningham Bryan W. , Brooks Daina M. , McAfee Paul C. 
Published: July 2024

AbstractEighty consecutive complex spinal robotic cases utilizing intraoperative 3D CT imaging (E3D, Group 2) were compared to 80 age-matched controls using the Excelsius robot alone with C-arm Fluoroscopic registration (Robot Only, Group 1). The demographics between the two groups were similar—severity of deformity, ASA Score for general anesthesia, patient age, gender, number of spinal levels instrumented, number of patients with prior spinal surgery, and amount of neurologic compression. The intraoperative CT scanning added several objective factors improving patient safety. There were significantly fewer complications in the E3D group with only 3 of 80 (4%) patients requiring a return to the operating room compared to 11 of 80 (14%) patients in the Robot Only Group requiring repeat surgery for implant related problems (Chi squared analysis = 5.00, p = 0.025). There was a significant reduction the amount of fluoroscopy time in the E3D Group (36 s, range 4–102 s) compared to Robot only group (51 s, range 15–160 s) (p = 0.0001). There was also shorter mean operative time in the E3D group (257 ± 59.5 min) compared to the robot only group (306 ± 73.8 min) due to much faster registration time (45 s). A longer registration time was required in the Robot only group to register each vertebral level with AP and Lateral fluoroscopy shots. The estimated blood loss was also significantly lower in Group 2 (mean 345 ± 225 ml) vs Group 1 (474 ± 397 ml) (p = 0.012). The mean hospital length of stay was also significantly shorter for Group 2 (3.77 ± 1.86 days) compared to Group 1 (5.16 ± 3.40) (p = 0.022). There was no significant difference in the number of interbody implants nor corrective osteotomies in both groups—Robot only 52 cases vs. 42 cases in E3D group.Level of evidence: IV, Retrospective review.
Exploring impact of entrepreneurial orientation on firm performance – moderators’ variability under changing market conditions
Authors: Suder Marcin , Kusa Rafał , Duda Joanna , Karpacz Jarosław 
Published: July 2024

Abstract
Previous research has indicated that the impact of entrepreneurial orientation (EO) on performance is ambiguous. This relationship can be affected by numerous factors – both internal and external. This study aims to examine the moderating impact of inter-organizational cooperation, competitive behaviors, digitalization, diversification, and flexibility on the relationship between EO and performance; it also assesses the impact of market conditions on the relationships that were examined above. The sample was comprised of 150 small printing companies, and the moderating roles were analyzed with PLS-SEM. The results confirmed the strong positive impact of EO on firm performance under both non-crisis and crisis conditions. The results indicated that, under crisis conditions, the impact of EO on market performance is positively moderated by inter-organizational cooperation, digitalization, and diversification. However, these factors do not moderate the examined relationship under non-crisis conditions – they only become moderators during a crisis. When supported with the Welch-Satterthwait statistical test, these observations indicated the moderating role of market conditions on the other factors that were examined in this study. With its findings, this study contributes to the literature on entrepreneurship and crisis management. The originality of the study is two-fold: first, this study examines the moderating impact of several factors that have not been previously tested on the EO–performance relationship; and second, it compares the examined models (and the entrepreneurial behaviors that are reflected in these models) and tests the moderating roles of the examined factors under two different market conditions (non-crisis, and crisis). In this way, the study tests the moderating role of market conditions as it relates to the examined moderators.
The Venezuelan Migrant Population’s Right to Health in the Bucaramanga Metropolitan Area
Authors: Serrano Frattali Juan Pablo 
Published: July 2024

AbstractColombia has received the largest influx of Venezuelan refugees and migrants. Since 2015, more than 3 million Venezuelan migrants have entered the country. Those arriving in Cúcuta have several options for entering Colombian territory. One of the main routes involves a difficult and dangerous journey of nearly 200 km to the Metropolitan Area of Bucaramanga, which serves as a key territory for accessing various destinations. Because of its geographical location, this area serves as an important transit city, facilitating travel to other major cities in Colombia, such as Bogotá, Medellín, and Cali; it is also a residence destination for Venezuelan migrants. Among the specific reasons why immigrants choose Bucaramanga over other cities are factors like climate, the existence of various cooperation organizations that provide assistance, and the considerable number of migrant individuals previously settled in this area; thus, many new migrants have family or friendship connections there. Furthermore, for many, it is the safest option or the only place they can go to when the weather turns cold. In this study, we analyze the institutional response and identify barriers that limit the protection of Venezuelan migrants’ right to access to healthcare in this location where Venezuelan citizens have faced economic, legal, bureaucratic, and social barriers. This has profound consequences for migrants’ health and poses a risk to their lives. The article is primarily based on interviews and fieldwork conducted with migrant populations between 2022 and 2023 to identify the barriers and supporting elements they experienced in terms of health care services. Likewise, this study makes a significant contribution by directly examining the experiences of Venezuelan migrants in relation to the right to health in this area. This provides a useful understanding of the social phenomenon at both the academic and institutional levels.
Comparative analysis of SPI, SPEI, and RDI indices for assessing spatio-temporal variation of drought in Türkiye
Authors: Öz Fatma Yaman , Özelkan Emre , Tatlı Hasan 
Published: July 2024

AbstractThis research presents a comprehensive drought analysis using climate data obtained from 219 homogeneously distributed meteorological stations in Türkiye between 1991 and 2022. In this context, Standard Precipitation Index (SPI), Standardized Precipitation Evapotranspiration Index (SPEI) and Reconnaissance Drought Index (RDI) drought indices were used and comparative analysis was made. Türkiye. The study demonstrates that below-normal precipitation over extended periods and increasing temperatures have contributed to the increased frequency of meteorological drought events. Türkiye's topographic conditions, particularly its location in the Mediterranean basin, significantly influence drought occurrences. It is noted that over the past 20 years, Türkiye has been trending towards drier conditions, with rising temperatures reinforcing this trend. The study observes that the moderate drought class range is the most frequently recurring in the SPI, SPEI, and RDI methods utilized. Regarding atmospheric conditions affecting the climate in Türkiye, it is observed that increased drought severity stands out prominently in years when the North Atlantic Oscillation is positive. During these years, increased drought severity is evident in the SPI, SPEI, and RDI indices, particularly in winter and autumn, while a wide area experiences drought effects in the summer months. Long-term analyses emphasize that drought periods occur less frequently but have more prolonged impacts, attributed to variations in precipitation patterns from year to year and the influence of rising temperatures due to global climate change. The potential future increase in drought in the Mediterranean basin due to global climate change and Türkiye's vulnerability to this situation could have adverse effects on water resources, food security, energy sources, and ecosystems.
Development and validation of a neoadjuvant chemotherapy pathological complete remission model based on Reg IV expression in breast cancer tissues: a clinical retrospective study
Authors: Wei Jiao-fei , Li Fan , Lin Jia-wen , Dou Zi-ang , Li Shu-qin , Shen Jun 
Published: July 2024

Abstract
Objective
To develop and authenticate a neoadjuvant chemotherapy (NACT) pathological complete remission (pCR) model based on the expression of Reg IV within breast cancer tissues with the objective to provide clinical guidance for precise interventions.

Method
Data relating to 104 patients undergoing NACT were collected. Variables derived from clinical information and pathological characteristics of patients were screened through logistic regression, random forest, and Xgboost methods to formulate predictive models. The validation and comparative assessment of these models were conducted to identify the optimal model, which was then visualized and tested.

Result
Following the screening of variables and the establishment of multiple models based on these variables, comparative analyses were conducted using receiver operating characteristic (ROC) curves, calibration curves, as well as net reclassification improvement (NRI) and integrated discrimination improvement (IDI). Model 2 emerged as the most optimal, incorporating variables such as HER-2, ER, T-stage, Reg IV, and Treatment, among others. The area under the ROC curve (AUC) for Model 2 in the training dataset and test dataset was 0.837 (0.734–0.941) and 0.897 (0.775–1.00), respectively. Decision curve analysis (DCA) and clinical impact curve (CIC) further underscored the potential applications of the model in guiding clinical interventions for patients.

Conclusion
The prediction of NACT pCR efficacy based on the expression of Reg IV in breast cancer tissue appears feasible; however, it requires further validation.
Long-Term Follow-Up before and during Riluzole Treatment in Six Patients from Two Families with Spinocerebellar Ataxia Type 7
Authors: Suppiej Agnese , Ceccato Chiara , Tzekov Radouil , Cermakova Iveta , Parmeggiani Francesco , Bellucci Gianmarco , Salvetti Marco , Zesiewicz Theresa , Ristori Giovanni , Romano Silvia 
Published: July 2024

Abstract
Background
Currently no curative treatment exists for spinocerebellar ataxias (SCAs). Riluzole repurposing was proposed as a symptomatic treatment in different types of cerebellar ataxia. We report a long-term-follow up under riluzole treatment in SCA type 7.

Methods
Six patients received Riluzole 50 mg twice daily on a compassionate use program for a mean of 4.8 years (range 3.5-9). We measured ataxia onset and progression through the Scale for the Assessment and Rating of Ataxia (SARA), and collected extensive ophthalmological data before and after Riluzole treatment. Electrocardiogram and laboratory profile for drug safety were performed every six months.

Results
Riluzole treatment showed no effect on visual function in two patients with an advanced retinal damage. Improvements of visual function occurred in four patients followed by ophthalmologic stability up to 5 years after starting treatment. Two patients had a less steep deterioration of ataxia after treatment compared to pre-treatment, during the first 2,5 years of therapy. One showed soon after therapy an improvement of the SARA score, and then overall stability lasting 3,5 years, followed by ataxia worsening. One visually impaired patient without neurological impairment did not worse until the last visit after 3,5 years of follow-up. The remaining 2 patients showed an improvement of SARA scores soon after therapy, and an overall stability lasting respectively 5 and 3 years. No adverse event was registered during the observation period.

Discussion
This study suggests a possible beneficial action of Riluzole in SCA7 and provides a detailed description of the ophthalmologic profile of these patients.