bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acetylcholinesterase inhibition mediated the larvicidal activity of Mangifera indica extract against Culex quinquefasciatus
Authors: Vadakkan Kayeen , Aravoor Sruthy Satheesan , Mundanttu Maya Rajan , Devanooru Bhavya Krishnamurthy , Puthiyamadathil Vidhya Mohanan 
Published: August 2024

AbstractThe study was formulated to identify the effect of Mangifera indica leaf extract in inhibiting the growth and metamorphosis of Culex quinquefasciatus larva. Bioassay-guided extraction identified the bioactive fraction, after which GC-MS characterized it. The larvicidal activity was analyzed by administrating extract in various concentrations and then subjecting the mortality rate for probit analysis. The morphological and physiological impact upon larvae was understood by histological analysis and acetylcholinesterase activity assay. The results suggested that the extract possessed a high degree of larvicidal activity, whereas the Dose50 was 225.158 ± 15.168 with a Total Chi-Square of 13.09 and p-value of 0.11. The histological studies revealed notable aberrations among the study subjects compared to the control group due to diminished abdominal tissue integrity.It was also observed that the extract could inhibit the acetylcholinesterase activity, with an LD 50 of 0.9512 µg/ml. The observations made in these studies may be utilized to develop a potential larvicidal agent that could act upon multiple targets.
Moving motivated by work or proximity to family and labour market outcomes in the Netherlands
Authors: Mulder Clara H. , Kooiman Niels 
Published: August 2024

AbstractThe classical human-capital model of migration posits that internal migration should lead to improved individual labour market outcomes. However, later empirical and theoretical work has emphasised that the outcomes of migration may be positive for some, but negative for others, depending on, for example, gender and the motive for moving. We investigate the labour market outcomes after moves motivated by work, moves motivated by proximity to family, and moves motivated by both work and family at the same time, compared with not moving. We use data from the Netherlands’ Housing Surveys of 2006, 2009, 2012, 2015, and 2018 matched with register data (N ~ 350,000 person-years). We find that men’s and women’s moves for work, and moves for both family and work, are positively associated with outcomes in terms of individual income, employment, and labour supply (hours worked). We do not find such positive associations for moves motivated by family proximity only, but we hardly find negative associations. We do not replicate the finding of previous research for Sweden that moves for family proximity were associated with an increased likelihood of transitions out of unemployment. However, we find some evidence that women’s moves motivated by both work and family proximity are associated with an increase in labour supply.
Optimization of transplantation methods using isolated mesenchymal stem/stromal cells: clinical trials of inflammatory bowel diseases as an example
Authors: Hisamatsu Daisuke , Ikeba Akimi , Yamato Taku , Mabuchi Yo , Watanabe Mamoru , Akazawa Chihiro 
Published: August 2024

AbstractMesenchymal stem/stromal cells (MSCs) are distributed in various tissues and are used in clinical applications as a source of transplanted cells because of their easy harvestability. Although MSCs express numerous cell-surface antigens, single-cell analyses have revealed a highly heterogeneous cell population depending on the original tissue and donor conditions, including age and interindividual differences. This heterogeneity leads to differences in their functions, such as multipotency and immunomodulatory effects, making it challenging to effectively treat targeted diseases. The therapeutic efficacy of MSCs is controversial and depends on the implantation site. Thus, there is no established recipe for the transplantation of MSCs (including the type of disease, type of origin, method of cell culture, form of transplanted cells, and site of delivery). Our recent preclinical study identified appropriate MSCs and their suitable transplantation routes in a mouse model of inflammatory bowel disease (IBD). Three-dimensional (3D) cultures of MSCs have been demonstrated to enhance their properties and sustain engraftment at the lesion site. In this note, we explore the methods of MSC transplantation for treating IBDs, especially Crohn’s disease, from clinical trials published over the past decade. Given the functional changes in MSCs in 3D culture, we also investigate the clinical trials using 3D constructs of MSCs and explore suitable diseases that might benefit from this approach. Furthermore, we discuss the advantages of the prospective isolation of MSCs in terms of interindividual variability. This note highlights the need to define the method of MSC transplantation, including interindividual variability, the culture period, and the transplantation route.
Synopsis of proposals on fungal nomenclature: a review of the proposals concerning Chapter F of the International Code of Nomenclature for algae, fungi, and plants submitted to the XII International Mycological Congress, 2024
Authors: May Tom W. , Bensch Konstanze 
Published: August 2024

AbstractA commentary is provided on the seven formally published proposals to modify the provisions of Chapter F of the International Code of Nomenclature for algae, fungi, and plants (ICNafp) that will be dealt with by the Fungal Nomenclature Session (FNS) of the 12th International Mycological Congress (IMC12) in August 2024. The proposals deal with: fungi whose morph-names have the same epithet; the listing of synonyms under entries for protected names in the Code Appendices; the processes of protection and rejection; the use of DNA sequences as nomenclatural types; the use of genomes as nomenclatural types; and the designation of fungi known only from DNA sequences. Information is also provided on the composition and role of the Fungal Nomenclature Bureau, the operation of the FNS and the pre-Congress Guiding vote.
From uncertain to certain—how to proceed with variants of uncertain significance
Authors: Banerjee Emili , Pal Suman , Biswas Abhijit , Bhattacharjee Koutilya 
Published: August 2024

AbstractWith the increased next generation sequencing (NGS) based genetic diagnosis due to technological boon, the biomedical world is getting a substantial number of single nucleotide variations (SNVs) every day along with other genetic variations. The detected SNVs may or may not have clinical significance. Based on different levels of study, these SNVs are categorized either as disease associated or not disease associated. However, there exists another category called as “uncertain” where the scientific literature has scanty of data. These “uncertain” or “variants of uncertain significance (VUS)” has become the greatest challenge for the diagnostic fraternity since no specific decision can be taken by them for the persons carrying the VUS. Therefore, there exists a huge knowledge gap that needs to be addressed for better patient care. The present study aims to find out the possible ways of investigation that may help in reducing this knowledge gap so that decisive approaches can be made against VUS for better and accurate patient care.
Relation between vitamin D and COVID-19 in Egyptian patients
Authors: Hammam Nour Hussein , Aziz Mona Ramadan Abdel , Soliman Alshaimaa Mohamed Mosaad , Aziz Sarah Younes Abdel , Altaweel Eman Hussein Soliman , Omran Asmaa M. A. , Nassar Mervat Ragab Abdel Rahman 
Published: August 2024

Abstract
Background
Insufficient vitamin D (VD) levels have been linked to a higher vulnerability to acute respiratory infections and the severity of COVID-19 sickness.

Objective
The purpose of this research is to investigate whether or not there is a connection between the amounts of VD produced by patients from Egypt and the severity of COVID-19, as well as the consequences of the disease.

Methods
This research used a case–control design and included a total of 90 adult patients who had been diagnosed with COVID-19, as well as 90 healthy controls who were matched in terms of age and sex. Patients were classified into mild, moderate, and severe categories according to clinical and radiological criteria. The study included measuring levels of VD and analyzing their relationships with illness severity, inflammatory markers, radiological findings, and outcomes.

Results
COVID-19 patient(s) had notably reduced levels of serum VD versus the control group (11.78 ± 3.24 ng/mL vs. 20.88 ± 7.76 ng/mL, p < 0.001). Lower VD levels were associated with more severe disease (p < 0.001), dyspnea (p < 0.001), radiological abnormalities (p = 0.001), and higher mortality (p < 0.001). A serum VD level ≤ 14.8 ng/mL could differentiate COVID-19 patients from controls with 86.67% sensitivity and 77.78% specificity (AUC = 0.881).

Conclusions
COVID-19 patients often had a deficiency of VD, which was linked to more severe illness, respiratory issues, aberrant radiological findings, and higher fatality rates. VD levels may be used as a biological surrogate marker to assess the risk and predict the outcome of COVID-19.
Ecofriendly treatments to improve pomegranate trees production and tolerance to climate change
Authors: Ahmed Mohamed F. , Ali Radwan Mohamed , Saad Zakaria Hassan , Ghaly Mohammed S. , Abd-Elhamed Waled , Alfawal Gamal Saleh 
Published: August 2024

Abstract

Background
Pomegranate is capable of growing in different agro-climatic conditions ranging from tropical to sub-tropical. Where vegetative growth, nutritional and physiological status of pomegranate plants oscillate at high temperatures waves in the summer season. While we can overcome the cracking and sunburn of fruits, which are the main problems confronting pomegranate cultivars due to their significant impact on the quality of fruits.

Methods

The present research was conducted over the course of two consecutive seasons in 2021 and 2022. The study focused on ten-year-old trees that were cultivated in sandy soil inside a private orchard situated 70 km away from Cairo on the Cairo-Alexandria desert road in Egypt (30
o
13′31.4″N30
o
39′09″E). The aim of the present work was to evaluate the effect of foliar spraying of Wonderful pomegranate trees with some nutrients (B, Zn, Fe, Mn, Ca and Cu), amino acids and irradiated licorice on vegetative growth, fruit physical, quality and chemical and leaf chemical parameters. A randomized full-block design with three duplicates was employed in the study. Using the recently computed least significant difference values at the 5% level, averages were compared.

Results
The results indicated that foliar spraying with nutrients, amino acids and licorice extract (irradiated or not) alone or in combination significantly increased the vegetative growth and leaf total chlorophyll content compared to the control. Also, they significantly increased fruit length, diameter, weight, and number/tree and thus yield. As compared to the control, all treatments significantly reduced the number of sunburned and cracked fruits leading to decrease the percentage of un-marketable fruits. The juice content of anthocyanin, total sugars and total soluble solids increased significantly in response to all treatments compared to the control while the acidity% greatly reduced. When these treatments were used instead of the control, the content of N, P, K, Fe, Zn, and Mn in the leaves was also positively increased.

Conclusions
In conclusion, foliar spraying of “Wonderful” pomegranate trees with a mixture of nutrients (B, Zn, Fe, Mn, Ca, Cu) and amino acids or irradiated licorice extract can be considered as a suitable management of the problem of sunburned or cracked fruits while improving the physical and chemical fruit quality with increased productivity.
Hot-electron dynamics in plasmonic nanostructures: fundamentals, applications and overlooked aspects
Authors: Khurgin Jacob , Bykov Anton Yu. , Zayats Anatoly V. 
Published: August 2024

AbstractLight absorption near a surface of conductive materials and nanostructures leads to the excitation of nonequilibrium, high-energy charge carriers: electrons above the Fermi level or holes below it. When remaining inside a material, these so-called hot carriers result in nonlinear, Kerr-type, optical effects important for controlling light with light. They can also transfer into the surroundings of the nanostructures, resulting in photocurrent, or they can interact with adjacent molecules and media, inducing photochemical transformations. Understanding the dynamics of hot carriers and related effects in plasmonic nanostructures is important for the development of ultrafast detectors and nonlinear optical components, broadband photocatalysis, enhanced nanoscale optoelectronic devices, nanoscale and ultrafast temperature control, and other technologies of tomorrow. In this review, we will discuss the fundamentals of plasmonically-engendered hot electrons, focusing on the overlooked aspects, theoretical descriptions and experimental methods to study them, and describe prototypical processes and examples of most promising applications of hot-electron processes at the metal interfaces.
A Multicenter Phase II Trial of Ruxolitinib for Treatment of Corticosteroid Refractory Sclerotic Chronic Graft-Versus-Host Disease
Authors: Bhatt Vijaya Raj , Shostrom Valerie K. , Choe Hannah K. , Hamilton Betty K. , Gundabolu Krishna , Maness Lori J. , Kumar Virender , Mahato Ram I. , Smith Lynette M. , Nishihori Taiga , Lee Stephanie J. 
Published: August 2024

PURPOSE
Sclerotic chronic graft-versus-host disease (cGVHD) represents a highly morbid and refractory form of cGVHD, and novel therapies for sclerotic cGVHD are critically needed. This study aimed to determine the efficacy of ruxolitinib in patients with corticosteroid refractory sclerotic cGVHD.

PATIENTS AND METHODS

In a single-arm multicenter phase II trial (N = 47), adults with sclerotic cGVHD refractory to corticosteroids and ≥one additional line of systemic therapy for cGVHD received ruxolitinib for ≥six months (ClinicalTrials.gov identifier:
NCT03616184
). The primary end point was complete or partial response (PR) in skin and/or joint defined according to the 2014 National Institute of Health cGVHD Consensus Criteria.

RESULTS
Following the use of ruxolitinib for a median of 11 months, PR in skin and/or joints was noted in 49% (95% CI, 34 to 64) at 6 months, with 45% having joint and fascia response and 19% having skin response. The duration of skin/joint response was 77% (95% CI, 48 to 91) at 12 months. Overall cGVHD PR was noted in 47% (95% CI, 32 to 61). Improvement in Lee Symptom Scale summary and skin subscale scores was noted in 38% of patients. With a cumulative incidence of treatment failure of 20.8% (95% CI, 10.0 to 34.1), nonrelapse mortality (NRM) of 2.2% (95% CI, 0.17 to 10.3), and no recurrent malignancy, failure-free survival (FFS) was 77.1% (95% CI, 61.3 to 87.0) at 12 months. Ruxolitinib was overall well tolerated with no new safety signals.

CONCLUSION
The use of ruxolitinib was associated with relatively high rates of skin/joint responses and overall cGVHD responses, improvement in patient-reported outcomes, low NRM, and high FFS in patients with refractory sclerotic cGVHD. Ruxolitinib offers an effective treatment option for refractory sclerotic cGVHD.
Non-Invasive, Continuous, Quantitative Detection of Solvent Content in Vacuum Tray Drying
Authors: Louge Michel Y. , Mandur Jasdeep , Grigorov Plamen , Blincoe William , Lamberto David , Bower Colton , Meyer Robert F. 
Published: August 2024

Abstract
A non-invasive capacitance instrument was embedded in the base of a vacuum-drying tray to monitor continuously the residual amount of solvent left in a pharmaceutical powder. Proof of concept was validated with Microcrystalline Cellulose laced with water, as well as water/acetone mixtures absorbed in a spray-dried Copovidone powder. To illustrate the role of impermeability of the base, we derive a model of vapor sorption that reveals the existence of a kinetic limit when solids are thinly spread, and a diffusion limit with greatly diminished effective diffusivity at large powder thickness. By monitoring the residual solvent content of powders, this new in situ technique offers advantages over indirect methods like mass spectrometry of vapor effluents, but without complications associated with probe fouling. To prescribe design guidelines and interpret signals, we model the electric field shed by the probe when a powder holds variable solvent mass fraction in the vertical direction.

Graphical abstract
Broadband seismic cloaks based on gradient index metamaterial
Authors: Tang Zepei , Zhang Yuexin , Linghu Zhiang , Yang Hongwu , Peng Pai , Liu Fengming , Du Qiujiao 
Published: August 2024

Abstract
In this work, we propose two gradient index seismic metamaterials (GRIN SMs) for realizing the directional cloaks composed of blind holes with varying radii in a semi-infinite soil substrate for seismic Rayleigh waves. The results demonstrate that the designed cloaks can transfer more than 50% of the incident wave energy before it reaches the target buildings. More importantly, the designed cloaks have the property of wide working bandwidth from 6 Hz to 12 Hz. At certain frequencies, the attenuation of incident waves by the cloak can reach 80%. Moreover, one of cloaks composed of four Luneburg lens can achieve a multi-directional seismic cloak.
Alteration of Bone Microarchitecture in Hereditary Distal RTA Patients With SLC4A1 Gene Mutation: Assessed by HR-pQCT
Authors: Chen Rong , Cui Lijia , Du Juan , Zhang Shujie , Jiang Yan , Li Mei , Xing Xiaoping , Wang Ou , Xia Weibo 
Published: August 2024

Abstract

Context
Hereditary distal renal tubular acidosis caused by SLC4A1 gene mutation (SLC4A1-dRTA) is a rare hereditary form of renal tubular acidosis. Rickets or osteomalacia is a common complication of SLC4A1-dRTA and seriously affects patients’ daily lives. However, studies on the bone microstructure in SLC4A1-dRTA are limited.

Objective
This work aimed to evaluate the bone microstructure of SLC4A1-dRTA patients, compared to age- and sex-matched healthy controls and X-linked hypophosphatemic rickets (XLH) patients.

Methods
This was a retrospective study of 11 SLC4A1-dRTA patients. Clinical manifestations and biochemical and radiographical examinations were characterized. Bone microstructure was examined in 7 SLC4A1-dRTA patients, 7 healthy controls, and 21 XLH patients using high-resolution peripheral quantitative computed tomography.

Results
Skeletal symptoms, including fracture, bone pain, and lower limb deformity, were present in 72.7% of SLC4A1-dRTA patients. Short stature was present in 63.6% of the patients. SLC4A1-dRTA patients had significantly lower volumetric bone mineral density in the distal tibia and more severe deteriorated trabecular bone in the distal radius and tibia than healthy controls. SLC4A1-dRTA patients had significantly more severely deteriorated trabecular bone in the distal radius and distal tibia compared to XLH patients. With long-term alkaline therapy, SLC4A1-dRTA patients had alleviated bone pain and increased height.

Conclusion
Skeletal lesions were common clinical manifestations in SLC4A1-dRTA patients. Compared with XLH, another common type of rickets, SLC4A1-dRTA patients had more severe trabecular bone microstructure damage, further supporting the necessity of early diagnosis and timely treatment of the disease.
Effects of Metabolic Syndrome on Pregnancy Outcomes in Women Without Polycystic Ovary Syndrome
Authors: Li Siyuan , Ma Shuxin , Yao Xiangyi , Liu Peihao 
Published: August 2024

Abstract

Context
Metabolic syndrome (MetS) is a cluster of metabolic risk factors that predict cardiovascular disease. Previous studies suggested that MetS impaired clinical outcomes in women with polycystic ovary syndrome (PCOS) undergoing in vitro fertilization (IVF).

Objective
To evaluate the effects of MetS on IVF/intracytoplasmic sperm injection (ICSI) outcomes in women without PCOS.

Methods
This retrospective study collected 8539 eligible women without PCOS who came for their first cycle of IVF/ICSI to the Institute of Women, Children and Reproductive Health, Shandong University, from 2017 to 2020, including 1147 subjects in the MetS group and 7392 in the control group. The primary outcome was live birth. Secondary outcomes included other pregnancy outcomes and the risk of maternal and neonatal complications.

Results
Women in the MetS group had a lower live birth rate (50.6% vs 54.9%, adjusted odds ratio [aOR] 0.87, 95% CI 0.75-1.00, P = .045) and higher risks of late miscarriage (5.8% vs 3.3%, aOR 1.52, 95% CI 1.02-2.27, P = .041), gestational diabetes mellitus (13.7% vs 7.0%, aOR 1.84, 95% CI 1.30-2.60, P = .001), hypertensive disorder of pregnancy (7.8% vs 3.5%, aOR 1.79, 95% CI 1.14-2.83, P = .012), and preterm birth (9.0% vs 4.4%, aOR 2.03, 95% CI 1.33-3.08, P = .001). Singleton newborns in the MetS group were at higher risk of large for gestational age (33.3% vs 20.5%, aOR 1.66, 95% CI (1.31-2.13), P &lt; .001) but at lower risk of small for gestational age (2.7% vs 6.2%, aOR 0.48, 95% CI 0.25-0.90, P = .023).

Conclusion
MetS was associated with adverse IVF/ICSI outcomes in women without PCOS.
Fragility Fractures and Cortisol Secretion in Patients With Nonfunctioning Adrenal Incidentalomas
Authors: Favero Vittoria , Cairoli Elisa , Eller-Vainicher Cristina , Morelli Valentina , Salcuni Antonio Stefano , Della Casa Silvia , Muscogiuri Giovanna , Columbu Carla , Pugliese Flavia , Corbetta Sabrina , Persani Luca , Scillitani Alfredo , Chiodini Iacopo 
Published: August 2024

Abstract

Context
The risk of vertebral fractures (VFx) in patients with nonfunctioning adrenal incidentalomas (NFAI) is unknown.

Objective
This work aimed to assess in NFAI patients the prevalence and incidence of VFx and a hormonal marker to identify patients at risk.

Methods
A retrospective, cross-sectional, and longitudinal study of outpatients was conducted. A total of 306 NFAI patients (cross-sectional arm) and 213 controls were evaluated for VFx prevalence; 85 NFAI patients (longitudinal arm, follow-up 30.3 ± 17.5 months) were evaluated for VFx incidence. Main outcome measures included serum cortisol after 1 mg-dexamethasone test (F-1mgDST), lumbar spinal (LS), and femoral neck (FN) bone mineral density (BMD) and VFx presence, by radiograph of the spine.

Results
Cross-sectional arm: prevalent VFx associated with F-1mgDST with a cutoff of 1.2 µg/dL (33 nmol/L, area under the curve 0.620 ± 0.39; P = .002). Compared with controls and NFAI patients with F-1mgDST less than 1.2 µg/dL (group A), NFAI patients with F-1mgDST greater than or equal to 1.2 µg/dL (group B) showed a higher VFx prevalence (10.8%, 12.6%, and 29.5%, respectively; P &lt; .001 all comparisons), which was associated with F-1mgDST greater than or equal to 1.2 µg/dL (odds ratio 3.02; 95% CI, 1.63-5.58; P &lt; .001) accounting to confounders. Longitudinal arm: the VFx incidence was higher in group B than in group A (19.3% vs 3.6%; P = .05). In group B, all incident VFx occurred in patients without low BMD. The F-1mgDST cutoff for predicting an incident VFx was 1.2 µg/dL, although statistical significance was not reached after adjustment for confounders (P = .061).

Conclusion
In NFAI patients, F-1mgDST levels greater than or equal to 1.2 µg/L (33 nmol/L) are associated with prevalent VFx and may identify patients at risk of incident VFx.