bluuebunny/crossref_metadata_2025_split huggingface dataset:
Photocatalytically induced hydrophilicity influences bone remodelling at longer healing periods: a rabbit study
Authors: Hayashi M. , Jimbo R. , Xue Y. , Mustafa K. , Andersson M. , Wennerberg A. 
Published: February 2013

AbstractObjectivesPreviously, we have reported that photocatalytically active hydrophilicity of the anatase titanium dioxide (TiO2) nanoparticles coated onto commercially pure titanium discs presented significantly improved hydrophilicity after ultraviolet irradiation. As hydrophilicity has shown enhancement of osseointegration, the in vivo responses were of great interest. The aim of this study was to evaluate whether or not the photo‐activated hydrophilicity generated at the time of implant placement has an effect on the longer healing periods for osseointegration.Materials and methodsPhotocatatytically active nanostructured TiO2 powder (Degussa P‐25), which consists of approximately 80% anatase and 20% rutile, was spin‐coated onto commercially pure titanium discs and was heat‐treated thereafter. These P25‐coated discs were irradiated with ultraviolet (UV) light for the test (+UV) group, and non‐irradiated discs were prepared for the control (−UV) group. Both groups of discs were placed in the rabbits' tibiae. After 12 weeks of healing period, histological analysis and gene expression analysis using real‐time RT‐PCR were performed.ResultsFrom the histological analyses, there were no specific differences between −UV and +UV groups. However, from the gene expression analysis, ALP, RUNX‐2 and IL‐10 were significantly upregulated for the +UV group compared with the −UV group.ConclusionsThe biologically enhancing effect to photocatalytically activated surfaces remained even after 12 weeks of healing time in terms of genetic responses.
Characterization and prognostic significance of mitochondrial DNA variations in acute myeloid leukemia
Authors: Silkjaer Trine , Nørgaard Jan Maxwell , Aggerholm Anni , Ebbesen Lene Hyldahl , Kjeldsen Eigil , Hokland Peter , Nyvold Charlotte Guldborg 
Published: February 2013

AbstractRecent studies have suggested that mutations in the mitochondrial genome (mtDNA) may play a role in the development and response to treatment for human cancer. The aim of this study was to investigate whether mtDNA variations have any prognostic relevance, to clarify the spectra of mtDNA variation and to determine whether there was any correlation to known prognostic factors in acute myeloid leukemia (AML). To elucidate this, we sequenced the entire mtDNA in 56 AML patients and 14 control subjects. When analyzing the biologic impact of the non‐synonymous variations in the mtDNA coding genes, we found an inferior disease‐free survival for patients exhibiting variations in the two most important catalytic genes of the complex IV of the oxidative phosphorylation complexes (OXPHOS), that is, the cytochrome c oxidase subunit I and the cytochrome c oxidase subunit II (hazard ratio 2.6, P = 0.03; multivariate analysis). In addition, the most frequent variation was the T16311C in the control region, which was found in 11 (20%) of the 56 patients. This observation was confirmed in another cohort of 173 diagnostic AML samples. In this expanded group, the T16311C variation tended to be associated with chromosomal abnormalities.
Canonical transient receptor potential channel subtype 3‐mediated hair cell Ca2+ entry regulates sound transduction and auditory neurotransmission
Authors: Wong Ann Chi Yan , Birnbaumer Lutz , Housley Gary D. 
Published: February 2013

AbstractThe physiological significance of canonical transient receptor potential (TRPC) ion channels in sensory systems is rapidly emerging. Heterologous expression studies show that TRPC3 is a significant Ca2+ entry pathway, with dual activation via G protein‐coupled receptor (GPCR)–phospholipase C–diacylglycerol second messenger signaling, and through negative feedback, whereby a fall in cytosolic Ca2+ releases Ca2+–calmodulin channel block. We hypothesised that the latter process contributes to cochlear hair cell cytosolic Ca2+ homeostasis. Confocal microfluorimetry with the Ca2+ indicator Fluo‐4 acetoxymethylester showed that, when cytosolic Ca2+ was depleted, Ca2+ re‐entry was significantly impaired in mature TRPC3−/− inner and outer hair cells. The impact of this disrupted Ca2+ homeostasis on sound transduction was assessed with the use of distortion product otoacoustic emissions (DPOAEs), which constitute a direct measure of the outer hair cell transduction that underlies hearing sensitivity and frequency selectivity. TRPC3−/− mice showed significantly stronger DPOAE (2f1 − f2) growth functions than wild‐type (WT) littermates within the frequency range of best hearing acuity. This translated to hyperacusis (decreased threshold) measured by the auditory brainstem response (ABR). TRPC3−/− and WT mice did not differ in the levels of temporary and permanent threshold shift arising from noise exposure, indicating that potential GPCR signaling via TRPC3 is not pronounced. Overall, these data suggest that the Ca2+ set‐point in the hair cell, and hence membrane conductance, is modulated by TRPC3s through their function as a negative feedback‐regulated Ca2+ entry pathway. This TPRC3‐regulated Ca2+ homeostasis shapes the sound transduction input–output function and auditory neurotransmission.
Effects of ozone in normal human epidermal keratinocytes
Authors: McCarthy James T. , Pelle Edward , Dong Kelly , Brahmbhatt Krupa , Yarosh Dan , Pernodet Nadine 
Published: February 2013

AbstractOzone is a tropospheric pollutant that can form at ground level as a result of an interaction between sunlight and hydrocarbon engine emissions. As ozone is an extremely oxidative reaction product, epidermal cells are in the outer layer of defense against ozone. We exposed normal human epidermal keratinocytes (NHEK) to concentrations of ozone that have been measured in cities and assayed for its effects. Hydrogen peroxide and IL‐1α levels both increased while ATP levels decreased. We found a decrease in the NAD‐dependent histone deacetylase, sirtuin 3. Lastly, we found that ozone increased DNA damage as evaluated by Comet assay. Taken together, our results show increased damage to NHEK that will ultimately impair normal cellular function as a result of an environmentally relevant ozone exposure.
Ca2+‐calmodulin interacts with DdCAD‐1 and promotes DdCAD‐1 transport by contractile vacuoles in Dictyostelium cells
Authors: Sriskanthadevan Shrivani , Brar Simuran K. , Manoharan Kumararaaj , Siu Chi‐Hung 
Published: February 2013

The Ca2+‐dependent cell–cell adhesion molecule DdCAD‐1, encoded by the cadA gene of Dictyostelium discoideum, is synthesized at the onset of development as a soluble protein and then transported to the plasma membrane by contractile vacuoles. Calmodulin associates with contractile vacuoles in a Ca2+‐dependent manner, and co‐localizes with DdCAD‐1 on the surface of contractile vacuoles. Bioinformatics analysis revealed multiple calmodulin‐binding motifs in DdCAD‐1. Co‐immunoprecipitation and pull‐down studies showed that only Ca2+‐bound calmodulin was able to bind DdCAD‐1. Structural integrity of DdCAD‐1, but not the native conformation, was required for its interaction with calmodulin. To investigate the role of calmodulin in the import of DdCAD‐1 into contractile vacuoles, an in vitro import assay consisting of contractile vacuoles derived from cadA− cells and recombinant proteins was employed. Prior stripping of the bound calmodulin from contractile vacuoles by EGTA impaired import of DdCAD‐1, which was restored by addition of exogenous calmodulin. The calmodulin antagonists W‐7 and compound 48/80 blocked the binding of calmodulin onto stripped contractile vacuoles, and inhibited the import of DdCAD‐1. Together, the data show that calmodulin forms a complex with DdCAD‐1 and promotes the docking and import of DdCAD‐1 into contractile vacuoles.Structured digital abstract

CaM physically interacts with DdCAD-1 by pull down (View Interaction: 1, 2)

DdCAD-1 binds to CaM by far western blotting (View interaction)

DdCAD-1 physically interacts with CaM by anti bait coimmunoprecipitation (View interaction)
Long‐term effects of aerobic training versus combined aerobic and resistance training in modifying cardiovascular disease risk factors in healthy elderly men
Authors: Sousa Nelson , Mendes Romeu , Abrantes Catarina , Sampaio Jaime , Oliveira José 
Published: February 2013

AimsThe purpose of the present study was to compare different modes of exercise in chronic modification of cardiovascular diseases risk factors.MethodsA total of 48 healthy elderly men were randomly assigned to an aerobic training group (n = 15, aged 71.7 ± 4.7 years), a combined (aerobic and resistance) training group (n = 16, aged 68.5 ± 3.5 years) and a control group (n = 17, aged 67.0 ± 5.8 years). Both training programs were of moderate‐to‐vigorous intensity, 3 days per week for 9 months. Primary outcome measures included body composition, blood pressure and lipid profile. Odds ratios (OR) between hypertension, obesity and dyslipidemia were calculated. The independence between risk factors, aggregation and group factor was tested (baseline vs post‐test).ResultsThere was significant aggregation between hypertension and obesity (OR 2.57, 95% CI 1.24–5.33). After 32 weeks, there was a significant change in the number of hypertensive (χ2 = 8.1, P = 0.004) and dyslipidemic (χ2 = 3.9, P = 0.049) participants, and also a favorable modification in the risk factors aggregation (χ2 = 7.9, P = 0.019), but only in the combined training group.ConclusionsCombined aerobic and resistance training is more effective in the chronic modification of blood pressure and lipid profile, and in the reduction of total risk factors aggregated. Geriatr Gerontol Int 2013; 13: 928–935.
‘From the time he took on the form of a servant’: The Christ's Pilgrimage of Faith
Authors: Allen Michael 
Published: February 2013

AbstractAddressing the pistis christou debate in New Testament studies, this article argues that in the eternal life of the perfect God, the divine Son pleases the Father in the Spirit and, therefore, the divine Son trusts the Father by the Spirit's power during his earthly pilgrimage. It roots soteriology in Christology and shows that the christological account flows forth from the eternal relations of the Trinity. The article concludes by noting implications for dogmatics and exegesis.
Extracorporeal shockwave lithotripsy for renal stones in pediatric patients: A multivariate analysis model for estimating the stone‐free probability
Authors: El‐Nahas Ahmed R , El‐Assmy Ahmed M , Awad Bassam A , Elhalwagy Samer M , Elshal Ahmed M , Sheir Khaled Z 
Published: February 2013

ObjectivesTo define factors affecting the stone‐free rate of extracorporeal shockwave lithotripsy in the treatment of pediatric renal calculi, and to establish a regression model for pretreatment prediction of stone‐free probability.MethodsFrom January 1999 through February 2012, 207 children with mean age 6.4 ± 3.8 years underwent shockwave lithotripsy with Dornier Lithotripter S for treatment of renal stones. The stone‐free rate was evaluated 3 months after the last shockwave lithotripsy session with non‐contrast computed tomography. Treatment success was defined as complete clearance of the stones with no residual fragments. Multivariate logistic regression analysis was used to identify independent risk factors and to predict the probability of being stone free.ResultsThe mean length of the stone was 11.6 ± 4 mm. The stone‐free rate was 71%. Independent factors that adversely affect stone‐free rate were increasing stone length and calyceal site of the stone. Relative risks for not being free of stones were 1.123 for stone length, 2.673 for stones in the upper or middle calyx and 4.208 for lower calyx stones.ConclusionStone length and location are prognostic factors determining stone‐free rate after shockwave lithotripsy for renal calculi in pediatric patients. Based on our analysis, shockwave lithotripsy should be recommended for renal pelvis stones up to 24 mm, upper or middle calyceal stones up to 15 mm and lower calyceal stones up to 11 mm.
Ambiguous genitalia and hypertension in a patient with congenital adrenal hyperplasia
Authors: Valsalan R. , Zimmermann A. 
Published: February 2013

AbstractCongenital adrenal hyperplasia (CAH) is an uncommon condition. Its clinical presentation with hypertension is rare. Deficiency of the steroid 11‐beta‐hydroxylase accounts for less than 10% of CAH. We report a case of a 19‐year‐old patient who presents with hypertension with ambiguous genitalia secondary to adrenal steroidogenesis dysfunction. We also discuss the defects in adrenal steroidogenesis and clinical phenotypes of CAH.
Renal Protective Effects of Early Continuous Venovenous Hemofiltration in Rhabdomyolysis: Improved Renal Mitochondrial Dysfunction and Inhibited Apoptosis
Authors: Tang Wanxin , Chen Zejun , Wu Weihua , Qiu Hongyu , Bo Hong , Zhang Ling , Fu Ping 
Published: February 2013

AbstractRhabdomyolysis (RM) and subsequent myoglobin (Mb) deposition can lead to acute kidney injury. Continuous venovenous hemofiltration (CVVH) can remove Mb, but direct renal protection is unclear. We hypothesized that CVVH can improve renal mitochondrial dysfunction in its early stage. Twenty‐four mongrel dogs were randomly divided into four groups: (A) control; (B) model; (C) model + CVVH (50 mL/kg/h); and (D) model + CVVH (30 mL/kg/h). RM was induced by glycerol via intramuscular injection. The dogs were closely monitored for urine flow and renal function. Mb, plasma tumor necrosis factor‐α (TNF‐α), and interleukin (IL)‐6 were measured by enzyme‐linked immunosorbent assay. After 8 h of CVVH, the morphological changes of renal mitochondria were observed and mitochondrial function indicators (reactive oxygen species, malondialdehyde, and respiratory control index) were detected. Western blot analysis was used to detect the expression of Mb, TNF‐α, and IL‐6 in renal tubules. The terminal deoxynucleotidyl transferase‐mediated dUTP‐biotin nick end labeling assay method and Western blot analysis were used to detect apoptosis and apoptosis‐related proteins. In group B, the dog urine output gradually decreased with increased blood creatinine. In groups C and D, the urine output was normal and stable. CVVH effectively eliminated Mb. High‐dose CVVH was significantly better for removal efficiency than low‐dose CVVH. CVVH significantly reduced the deposition of circulating Mb in the kidney in a dose‐dependent manner. The impact of CVVH on TNF‐α and IL‐6 were not observed. The morphological changes of mitochondria and function indicators were significantly improved in group C compared with groups D and B. Compared with group B, renal apoptosis and apoptosis‐related protein expression were inhibited in groups C and D. Group C was significantly better for mitochondrial improvement and apoptosis inhibition than group D. At the cellular and molecular level, CVVH can improve renal mitochondrial function and inhibit cell apoptosis. Early CVVH can protect from RM‐caused renal injuries in a dose‐dependent manner.
Comparison and Experimental Validation of Fluid Dynamic Numerical Models for a Clinical Ventricular Assist Device
Authors: Zhang Jiafeng , Zhang Pei , Fraser Katharine H. , Griffith Bartley P. , Wu Zhongjun J. 
Published: February 2013

AbstractWith the recent advances in computer technology, computational fluid dynamics (CFDs) has become an important tool to design and improve blood‐contacting artificial organs, and to study the device‐induced blood damage. Commercial CFD software packages are readily available, and multiple CFD models are provided by CFD software developers. However, the best approach of using CFD effectively to characterize fluid flow and to predict blood damage in these medical devices remains debatable. This study aimed to compare these CFD models and provide useful information on the accuracy of each model in modeling blood flow in circulatory assist devices. The laminar and five turbulence models (Spalart‐Allmaras, k‐ε (k‐epsilon), k‐ω (k‐omega), SST [Menter's Shear Stress Transport], and Reynolds Stress) were implemented to predict blood flow in a clinically used circulatory assist device, the CentriMag centrifugal blood pump. In parallel, a transparent replica of the CentriMag pump was constructed and selected views of the flow fields were measured with digital particle image velocimetry (DPIV). CFD results were compared with the DPIV experimental results. Compared with the experiment, all the selected CFD models predicted the flow pattern fairly well except the area of the outlet. However, quantitatively, the laminar model results were the most deviated from the experimental data. On the other hand, k‐ε renormalization group theory models and Reynolds Stress model are the most accurate. In conclusion, for the circulatory assist devices, turbulence models provide more accurate results than the laminar model. Among the selected turbulence models, k‐ε and Reynolds Stress Method models are recommended.
No increased sperm DNA fragmentation index in semen containing human papillomavirus or herpesvirus
Authors: Kaspersen M. D. , Bungum M. , Fedder J. , Bonde J. , Larsen P. B. , J Ingerslev H. , Höllsberg P. 
Published: February 2013

SummaryIt remains unknown whether human papillomaviruses (HPVs) or human herpesviruses (HHVs) in semen affect sperm DNA integrity. We investigated whether the presence of these viruses in semen was associated with an elevated sperm DNA fragmentation index. Semen from 76 sperm donors was examined by a PCR‐based hybridization array that identifies all HHVs and 35 of the most common HPVs. Sperm DNA integrity was determined by the sperm chromatin structure assay. HPVs or HHVs, or both, were found in 57% of semen samples; however, sperm DNA fragmentation index was not increased in semen containing these viruses.
Cardiovascular Magnetic Resonance Demonstrates Reversible Atrial Dysfunction After Catheter Ablation of Persistent Atrial Fibrillation
Authors: MUELLERLEILE KAI , GROTH MICHAEL , STEVEN DANIEL , HOFFMANN BORIS A. , SARING DENNIS , RADUNSKI ULF K. , LUND GUNNAR K. , ADAM GERHARD , ROSTOCK THOMAS , WILLEMS STEPHAN 
Published: February 2013

Reversible Dysfunction After Persistent AF AblationIntroductionThere is a paucity of data on atrial injury following ablation of persistent atrial fibrillation (AF). This study aimed at assessing reversibility of atrial dysfunction after successful persistent AF ablation using cardiovascular magnetic resonance (CMR).Methods and ResultsCMR was performed during sinus rhythm (SR) in 20 consecutive patients with persistent AF at baseline (BL) within 24 hours after ablation and after 6‐month follow‐up (FU). Catheter ablation included atrial substrate modification using the stepwise approach following pulmonary vein isolation (PVI) in order to attempt termination of persistent AF. Active left (LA) and right atrial (RA) function were quantified by calculating the active emptying fraction (AEF) from transvalvular flow profiles using velocity encoded (VENC) CMR. LA appendage (LAA) function was quantified by measurements of peak a‐wave velocities from flow profiles perpendicular to the LAA orifice. Peri‐atrial edema was assessed using black‐blood T2‐weighted CMR. A significant improvement was found in LA‐AEF from 18 (12–26)% at BL to 25 (22–35)% at FU (P = 0.0001). Furthermore, RA‐AEF significantly increased from 31 (19–35)% at BL to 40 (35–51)% at FU (P < 0.0001). A significant improvement was also found for LAA a‐wave velocities from 45 (31–65) cm/s at BL to 62 (49–75) cm/s at FU (P < 0.01). The area of peri‐atrial edema on T2‐weighted CMR decreased from 1393 (1098–1797) mm2 at BL to 24 (1–92) mm2 at FU (P < 0.0001).ConclusionCMR demonstrates reversibility of LA, LAA, and RA dysfunction associated with resorption of peri‐atrial edema in patients with SR after persistent AF ablation.
Characteristics of Atrial Fibrillation and Comorbidities in Familial Atrial Fibrillation
Authors: JURKKO RAIJA , PALOJOKI EEVA , HUTTUNEN HANNA , HOLM CHRISTIAN , LEHTO MIKA , HELIÖ TIINA , SWAN HEIKKI , TOIVONEN LAURI 
Published: February 2013

Familial Atrial FibrillationIntroductionOne‐third of lone atrial fibrillation (AF) presents as familial disorder. Heterogeneity of both genetic background and clinical manifestations remains largely uncharacterized. We aimed to evaluate the clinical characteristics and especially the triggering factors of familial AF.Methods and ResultsProbands were screened from 84 consecutive lone AF patients seen in our tertiary hospital arrhythmia clinic. Those confirmed to have at least 1 first‐degree relative with lone AF were included. 12‐lead ECG, Holter recording, and cardiac ultrasound were performed. Data concerning arrhythmias and other medical history were collected. Altogether 17 kindreds with 59 AF patients, 52 of whom had lone AF, were identified. Initiation of AF was atrial extrasystolia (PACs) related in 35%, and vagal or sympathetic in 30% of cases. Within any given family, the characteristics related to AF initiation were the same in two‐thirds of kindreds. AV conduction abnormalities were found in 2 families, sinus node dysfunction in 2 families, and both in 3 families. Frequent premature ventricular complexes (>1,000/24 hours) were observed in 9 families. Additional comorbidities included dilative cardiomyopathy and sudden death in 3 families.ConclusionsIn familial AF the proportion of PACs‐related AF is lower than expected. The arrhythmia triggers for lone AF in general are heterogeneous but often family specific. Concomitant rhythm disorders, as well as cardiomyopathies, are common in patients with familial AF. A positive family history for AF in an apparently lone AF patient may be a marker for wider spectrum of cardiac pathology.
Phenolic Composition Analysis and Gene Expression in Developing Seeds of Yellow‐ and Black‐seeded Brassica napus
Authors: Jiang Jinjin , Shao Yanlin , Li Aimin , Lu Chunliang , Zhang Yongtai , Wang Youping 
Published: February 2013

AbstractBreeders have focused on yellow‐seeded Brassica napus (rapeseed) for its better quality compared with the black‐seeded variety. Moreover, flavonoids have been associated with this kind of rapeseed. In this study, we applied lipid chromatography‐electrospray ionization mass spectrometry (LC‐ESI‐MSn) to compare flavonoids in developing seeds of natural black‐seeded B. napus and yellow‐seeded introgression lines selected from progenies of B. napus–Sinapis alba somatic hybrids. Aside from the most abundant phenolic compounds (sinapine and sinapic acid) and 1, 2‐disinapoylglucose, 16 different flavonoids were identified and quantified, including (‐)‐epicatechin, five monocharged oligomers of (‐)‐epicatechin ([DP 2]−, [DP 3]−, [DP 4] [DP 2]−B2 and [DP 2]−B5), quercetin, kaempferol, isorhamnetin‐dihexoside, kaempferol‐sinapoyl‐trihexoside, isorhamnetin‐sinapoyl‐trihexoside, isorhamnetin‐hexoside‐sulfate, and isorhamnetin‐3‐O‐glucoside. Most of the flavonoids accumulated with seed development, whereas some rapidly decreased during maturation. The content of these flavonoids were lower in the yellow‐seeded materials than in the black seeds. In addition, variations of insoluble procyanidin oligomers and soluble phenolic acids were observed among both rapeseed varieties. Transcriptome changes of genes participating in the flavonoid pathway were discovered by quantitative reverse transcription polymerase chain reaction analysis. Consistent with flavonoid changes identified by high performance liquid chromatography analysis, the expression of most genes in the flavonoid biosynthetic pathway was also downregulated.