bluuebunny/crossref_metadata_2025_split huggingface dataset:
Supraspinal Characterization of the Thermal Grill Illusion with fMRI
Authors: Leung Albert , Shukla Shivshil , Li Eric , Duann Jeng-Ren , Yaksh Tony 
Published: March 2014

Background: Simultaneous presentation of non-noxious warm (40°C) and cold (20°C) stimuli in an interlacing fashion results in a transient hot burning noxious sensation (matched at 46°C) known as the thermal grill (TG) illusion. Functional magnetic resonance imaging and psychophysical assessments were utilized to compare the supraspinal events related to the spatial summation effect of three TG presentations: 20°C/20°C (G2020), 20°C/40°C (G2040) and 40°C/40°C (G4040) with corresponding matched thermode stimuli: 20°C (P20), 46°C (P46) and 40°C (P40) and hot pain (HP) stimuli. Results: For G2040, the hot burning sensation was only noted during the initial off-line assessment. In comparison to P40, G4040 resulted in an equally enhanced response from all supraspinal regions associated with both pain sensory/discriminatory and noxious modulatory response. In comparison to P20, G2020 presentation resulted in a much earlier diminished/sedative response leading to a statistically significantly (P < 0.01) higher degree of deactivation in modulatory supraspinal areas activated by G4040. Granger Causality Analysis showed that while thalamic activation in HP may cast activation inference in all hot pain related somatosensory, affective and modulatory areas, similar activation in G2040 and G2020 resulted in deactivation inference in the corresponding areas. Conclusions: In short, the transient TG sensation is caused by a dissociated state derived from non-noxious warm and cold spatial summation interaction. The observed central dissociated state may share some parallels in certain chronic neuropathic pain states.
Fibroblast growth factor receptor splice variants are stable markers of oncogenic transforming growth factor β1 signaling in metastatic breast cancers
Authors: Wendt Michael K , Taylor Molly A , Schiemann Barbara J , Sossey-Alaoui Khalid , Schiemann William P 
Published: March 2014

Abstract
Introduction
Epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial transition (MET) facilitate breast cancer (BC) metastasis; however, stable molecular changes that result as a consequence of these processes remain poorly defined. Therefore, with the hope of targeting unique aspects of metastatic tumor outgrowth, we sought to identify molecular markers that could identify tumor cells that had completed the EMT:MET cycle.

Methods
An in vivo reporter system for epithelial cadherin (E-cad) expression was used to quantify its regulation in metastatic BC cells during primary and metastatic tumor growth. Exogenous addition of transforming growth factor β1 (TGF-β1) was used to induce EMT in an in situ model of BC. Microarray analysis was employed to examine gene expression changes in cells chronically treated with and withdrawn from TGF-β1, thus completing one full EMT:MET cycle. Changes in fibroblast growth factor receptor type 1 (FGFR1) isoform expression were validated using PCR analyses of patient-derived tumor tissues versus matched normal tissues. FGFR1 gene expression was manipulated using short hairpin RNA depletion and cDNA rescue. Preclinical pharmacological inhibition of FGFR kinase was employed using the orally available compound BGJ-398.

Results
Metastatic BC cells undergo spontaneous downregulation of E-cad during primary tumor growth, and its expression subsequently returns following initiation of metastatic outgrowth. Exogenous exposure to TGF-β1 was sufficient to drive the metastasis of an otherwise in situ model of BC and was similarly associated with a depletion and return of E-cad expression during metastatic progression. BC cells treated and withdrawn from TGF-β stably upregulate a truncated FGFR1-β splice variant that lacks the outermost extracellular immunoglobulin domain. Identification of this FGFR1 splice variant was verified in metastatic human BC cell lines and patient-derived tumor samples. Expression of FGFR1-β was also dominant in a model of metastatic outgrowth where depletion of FGFR1 and pharmacologic inhibition of FGFR kinase activity both inhibited pulmonary tumor outgrowth. Highlighting the dichotomous nature of FGFR splice variants and recombinant expression of full-length FGFR1-α also blocked pulmonary tumor outgrowth.

Conclusion
The results of our study strongly suggest that FGFR1-β is required for the pulmonary outgrowth of metastatic BC. Moreover, FGFR1 isoform expression can be used as a predictive biomarker for therapeutic application of its kinase inhibitors.
Should we breathe quiet or noisy?
Authors: Putensen Christian , Muders Thomas 
Published: March 2014

AbstractExternal noise is introduced by computer-generated random levels of pressure assistance during noisy pressure support ventilation (PSV). In patients, noisy PSV was associated with higher tidal volume variability but not improved cardio-pulmonary function compared with conventional PSV. The potential role of noisy PSV in the management of critically ill patients requiring ventilatory support has to be explored further.
Highly enhanced optical properties of indocyanine green/perfluorocarbon nanoemulsions for efficient lymph node mapping using near-infrared and magnetic resonance imaging
Authors: Bae Pan Kee , Jung Juyeon , Chung Bong Hyun 
Published: March 2014

AbstractThe near-infrared (NIR) fluorescence probe has better tissue penetration and lower autofluorescence. Indocyanine green (ICG) is an NIR organic dye for extensive biological application, and it has been clinically approved for human medical imaging and diagnosis. However, application of this dye is limited by its numerous disadvantageous properties in aqueous solution, including its concentration-dependent aggregation, poor aqueous stability in vitro, and low quantum yield. Its use in molecular imaging probes is limited because it loses fluorescence after binding to nonspecific plasma proteins, leading to rapid elimination from the body with a half-life of 2 – 4 min. In this study, the multifunctional perfluorocarbon (PFC)/ICG nanoemulsions were investigated with the aim of overcoming these limitations. The PFC/ICG nanoemulsions as a new type of delivery vehicle for contrast agents have both NIR optical imaging and19 F-MR imaging moieties. These nanoemulsions exhibited less aggregation, increased fluorescence intensity, long-term stability, and physicochemical stability against external light and temperature compared to free aqueous ICG. Also, the PFC/ICG bimodal nanoemulsions allow excellent detection of lymph nodes in vivo through NIR optical imaging and19 F-MR imaging. This result showed the suitability of the proposed nanoemulsions for non-invasive lymph node mapping as they enable long-time detection of lymph nodes.
Circulating Tumor Cell Counts Are Prognostic of Overall Survival in SWOG S0421: A Phase III Trial of Docetaxel With or Without Atrasentan for Metastatic Castration-Resistant Prostate Cancer
Authors: Goldkorn Amir , Ely Benjamin , Quinn David I. , Tangen Catherine M. , Fink Louis M. , Xu Tong , Twardowski Przemyslaw , Van Veldhuizen Peter J. , Agarwal Neeraj , Carducci Michael A. , Monk J. Paul , Datar Ram H. , Garzotto Mark , Mack Philip C. , Lara Primo , Higano Celestia S. , Hussain Maha , Thompson Ian Murchie , Cote Richard J. , Vogelzang Nicholas J. 
Published: March 2014

Purpose Circulating tumor cell (CTC) enumeration has not been prospectively validated in standard first-line docetaxel treatment for metastatic castration-resistant prostate cancer. We assessed the prognostic value of CTCs for overall survival (OS) and disease response in S0421, a phase III trial of docetaxel plus prednisone with or without atrasentan. Patients and Methods CTCs were enumerated at baseline (day 0) and before cycle two (day 21) using CellSearch. Baseline counts and changes in counts from day 0 to 21 were evaluated for association with OS, prostate-specific antigen (PSA), and RECIST response using Cox regression as well as receiver operator characteristic (ROC) curves, integrated discrimination improvement (IDI) analysis, and regression trees. Results Median day-0 CTC count was five cells per 7.5 mL, and CTCs < versus ≥ five per 7.5 mL were significantly associated with baseline PSA, bone pain, liver disease, hemoglobin, alkaline phosphatase, and subsequent PSA and RECIST response. Median OS was 26 months for < five versus 13 months for ≥ five CTCs per 7.5 mL at day 0 (hazard ratio [HR], 2.74 [adjusting for covariates]). ROC curves had higher areas under the curve for day-0 CTCs than for PSA, and IDI analysis showed that adding day-0 CTCs to baseline PSA and other covariates increased predictive accuracy for survival by 8% to 10%. Regression trees yielded new prognostic subgroups, and rising CTC count from day 0 to 21 was associated with shorter OS (HR, 2.55). Conclusion These data validate the prognostic utility of CTC enumeration in a large docetaxel-based prospective cohort. Baseline CTC counts were prognostic, and rising CTCs at 3 weeks heralded significantly worse OS, potentially serving as an early metric to help redirect and optimize therapy in this clinical setting.
Multicenter Phase II Study of Mogamulizumab (KW-0761), a Defucosylated Anti-CC Chemokine Receptor 4 Antibody, in Patients With Relapsed Peripheral T-Cell Lymphoma and Cutaneous T-Cell Lymphoma
Authors: Ogura Michinori , Ishida Takashi , Hatake Kiyohiko , Taniwaki Masafumi , Ando Kiyoshi , Tobinai Kensei , Fujimoto Katsuya , Yamamoto Kazuhito , Miyamoto Toshihiro , Uike Naokuni , Tanimoto Mitsune , Tsukasaki Kunihiro , Ishizawa Kenichi , Suzumiya Junji , Inagaki Hiroshi , Tamura Kazuo , Akinaga Shiro , Tomonaga Masao , Ueda Ryuzo 
Published: March 2014

Purpose CC chemokine receptor 4 (CCR4) is expressed by peripheral T-cell lymphomas (PTCLs) and is associated with poor outcomes. Mogamulizumab (KW-0761) is a defucosylated humanized anti-CCR4 antibody engineered to exert potent antibody-dependent cellular cytotoxicity. This multicenter phase II study evaluated the efficacy and safety of mogamulizumab in patients with relapsed PTCL and cutaneous T-cell lymphoma (CTCL). Patients and Methods Mogamulizumab (1.0 mg/kg) was administered intravenously once per week for 8 weeks to patients with relapsed CCR4-positive PTCL or CTCL. The primary end point was the overall response rate, and the secondary end points included safety, progression-free survival (PFS), and overall survival (OS). Results A total of 38 patients were enrolled, and 37 patients received mogamulizumab. Objective responses were noted for 13 of 37 patients (35%; 95% CI, 20% to 53%), including five patients (14%) with complete response. The median PFS was 3.0 months (95% CI, 1.6 to 4.9 months), and the median OS was not calculated. The mean maximum and trough mogamulizumab concentrations (± standard deviation) after the eighth infusion were 45.9 ± 9.3 and 29.0 ± 13.3 μg/mL, respectively. The most common adverse events were hematologic events, pyrexia, and skin disorders, all of which were reversible and manageable. Conclusion Mogamulizumab exhibited clinically meaningful antitumor activity in patients with relapsed PTCL and CTCL, with an acceptable toxicity profile. Further investigation of mogamulizumab for treatment of T-cell lymphoma is warranted.
A Community-Based Study on the Association Between Insomnia and Hypothalamic-Pituitary-Adrenal Axis: Sex and Pubertal Influences
Authors: Zhang Jihui , Lam Siu-Ping , Li Shirley Xin , Ma Ronald C. W. , Kong Alice P. S. , Chan Michael H. M. , Ho Chung-Shun , Li Albert M. , Wing Yun-Kwok 
Published: March 2014

Context:The association between insomnia disorder and the hypothalamic-pituitary-adrenal (HPA) axis needs to be explored in both adults and adolescents.Objectives:Our objective was to investigate the associations of the HPA axis (via serial salivary cortisol) with insomnia disorder and subjective and objective sleep quality in a community-based study.Design and Setting:This was a community-based case-control family study.Participants:Participants included 205 adolescents (14.2 ± 2.8 years old, 51.7% females, and 57 with insomnia) and 244 adults (46.4 ± 4.1 years old, 52.8% females, and 69 with insomnia).Main Outcome Measures:Outcome measures included a diagnostic interview for assessment of insomnia disorder, 3-day actigraphy and sleep diary, and serial salivary cortisol measurement.Results:Adults with insomnia had a significantly greater cortisol awakening response (CAR) reference to increase (CARi) but a comparable CAR reference to ground and a comparable cortisol level during afternoon and evening when compared with noninsomniac adults. The association between insomnia disorder and larger CARi was also found in adolescents at late/post puberty but not in pre/early pubertal adolescents. There was an interaction effect between sex and insomnia disorder on CARi level with adult females having larger CARi than adult males. Among subjects with insomnia disorder, those with lower subjective sleep efficiency had higher cortisol levels in the late evening (10:00 pm) in both adults and adolescents.Conclusions:Our study suggests that a series of insomniac indices at both syndromal and symptomatic levels including clinical diagnosis and poor sleep quality are associated with dysfunction of the HPA axis. The association between insomnia and increased CARi emerges at late puberty, and the sex difference in this association occurs in adulthood but not in adolescence.
Loss of CDC14B Expression in Clear Cell Renal Cell Carcinoma
Authors: Kim Younghye , Choi Jung-Woo , Lee Ju-Han , Kim Young-Sik 
Published: March 2014

Abstract

Objectives:
To discover significant differentially expressed genes (DEGs) in clear cell renal cell carcinoma (ccRCC) that might be unidentified by single microarray analysis.

Methods:
The effect sizes of five ccRCC microarray data sets were combined using a random-effects model. The most downregulated gene was validated in paired 80 ccRCC tissues by immunohistochemistry.

Results:
CDC14B was the most downregulated gene among 1,761 DEGs. CDC14B was strongly expressed in the apical proximal tubules in the nonneoplastic tissues, while it was completely absent in 10 (12.5%) of 80 or downregulated in 70 (87.5%) of 80 ccRCC cases. The complete loss of CDC14B correlated with high T stage (P = .038), advanced TNM stage (P = .027), tumor recurrence (P = .038), and shorter recurrence-free survival (P = .046) compared with the partial loss of CDC14B.

Conclusions:
Microarray meta-analysis is a useful tool for pathologists. CDC14B expression is downregulated in ccRCC, suggesting its role in renal carcinogenesis.
Trends in RBC Ordering and Use After Implementing Adaptive Alerts in the Electronic Computerized Physician Order Entry System
Authors: McWilliams Brian , Triulzi Darrell J. , Waters Jonathan H. , Alarcon Louis H. , Reddy Vivek , Yazer Mark H. 
Published: March 2014

Abstract

Objectives:
To reduce unnecessary RBC transfusions, the computerized physician order entry (CPOE) system was programmed to require prescribers to select an indication for transfusion. An alert appeared if the patient’s hemoglobin (Hb) level was above the threshold determined by the selected indication (adaptive alerts).

Methods:
Data on RBC orders from the 4 months before the adaptive alerts were implemented were compared with the 10 months after implementation.

Results:
Significantly fewer alerts were generated after the implementation, and the rate at which the alerts were heeded also increased. There was a trend toward fewer RBC units transfused after adaptive alert implementation. A large number of RBCs were ordered using a nonspecific transfusion indication, although many of these patients had antecedent Hb values close to the threshold.

Conclusions:
Electronically generated alerts can reduce but are insufficient to eliminate non–evidence-based transfusions. Analysis of the alerts suggests areas for CPOE improvement.
CSF-1R Expression in Tumor-Associated Macrophages Is Associated With Worse Prognosis in Classical Hodgkin Lymphoma
Authors: Koh Young Wha , Park Chansik , Yoon Dok Hyun , Suh Cheolwon , Huh Jooryung 
Published: March 2014

Abstract

Objectives:
The aim of this study was to determine the prognostic relevance of colony-stimulating 1 receptor (CSF-1R) expression in both Hodgkin/Reed-Sternberg (HRS) cells and the surrounding cells (non-HRS cells) in patients with classical Hodgkin lymphoma (CHL) .

Methods:
Diagnostic tissues from 112 patients with CHL treated with doxorubicin, bleomycin, vinblastine, and dacarbazine were evaluated retrospectively by immunohistochemical analysis for CSF-1R and CD68 and CD163 for tissue-associated macrophages.

Results:
High numbers (≥30%) of non-HRS cells expressing CSF-1R conferred inferior event-free survival and overall survival in univariate and multivariate analysis. High numbers of non-HRS cells expressing CSF-1R were significantly associated with a high number of tumor-associated macrophages as detected by CD163 expression (P &lt; .001). In particular, coexpression of CSF-1R and CD163 was associated with a worse survival outcome than either CSF-1R or CD163 expression alone or no expression.

Conclusions:
Our data demonstrate that a high number of non-HRS cells expressing CSF-1R are correlated with an increased tumor macrophage content and worse survival.
Nutrient concentration of down woody debris in mixedwood forests in central Maine, USA
Authors: Saunders Mike , Fraver Shawn , Wagner Robert 
Published: March 2014

Both nutrient concentrations and pre- and post-harvest pool sizes were determined across down woody debris decay classes of several hardwood and softwood species in a long-term, natural disturbance based, silvicultural experiment in central Maine. Concentrations of N, P, Ca, Mg, Cu, Fe, and Zn generally increased 2- to 5-fold with increasing decay class. Concentrations of Mn, Al and B did not differ among decay classes, while K decreased by 20â44% from decay class 1 to class 4. C:N-ratios declined with increasing decay class, while N:P-ratios increased from decay class 1 to 2 and then plateaued with further decay. Within decay classes, softwoods generally had lower nutrient concentrations and higher C:N-ratios than hardwoods; N:P-ratios did not differ between hardwoods and softwoods. Although gap harvesting increased the size of the overall down woody debris nutrient pools, mostly through a large pulse of decay class 1 material, harvesting generally reduced the nutrients held in advanced decay classes. Pre-harvest down woody debris pools for N, P, K and Ca were 11.0, 0.6, 2.1 and 21.1 kg ha, respectively, while postharvest were 20.0, 1.3, 6.2 and 46.2 kgÂ ha, respectively. While the gap-based silvicultural systems sampled in this study doubled the size of the pre-harvest, downed woody debris nutrient pools, the post-harvest pools were estimated to be only 3.2â9.1% of aboveground nutrients.â1â1
Jumping pairs of Steiner bundles
Authors: Arrondo Enrique , Marchesi Simone 
Published: March 2014

Abstract
In this work we introduce the definition of Schwarzenberger bundle on a Grassmannian. Recalling the notion of Steiner bundle, we generalize the concept of jumping pair for a Steiner bundle on a Grassmannian. After studying the jumping locus variety and bounding its dimension, we give a complete classification of Steiner bundles with jumping locus of maximal dimension, which all are Schwarzenberger bundles.
Neonatal serum magnesium concentrations are determined by total maternal dose of magnesium sulfate administered for neuroprotection
Authors: Borja-Del-Rosario Pamela , Basu Sudeepta Kumar , Haberman Shoshana , Bhutada Alok , Rastogi Shantanu 
Published: March 2014

Abstract

Background: Antenatal magnesium in preterm labor for neuroprotection decreases the incidence of cerebral palsy. However, there are no guidelines on the dose and duration of magnesium infusion for neuroprotection. As increased neonatal serum magnesium concentrations may be related to higher risk of morbidity and mortality, the role of total amount of magnesium and maternal serum magnesium concentrations associated with safe neonatal serum magnesium concentrations is not known.

Methods: A retrospective study was conducted on 289 mothers who received antenatal magnesium for neuroprotection as a loading dose of 4–6 g infused over 30 min, followed by a maintenance infusion of 1–2 g/h. Total magnesium dose infused to the mother and maternal serum magnesium concentrations were correlated with neonatal serum magnesium concentrations.

Results: Of the 289 mothers, 192 mother/baby dyads had all three measurements (maternal total magnesium dose, and maternal and neonatal serum magnesium concentrations). Magnesium infusion was continued beyond 24 h in 60 mothers. Total maternal magnesium dose at 24 and 48 h of infusion correlated with neonatal serum magnesium concentrations (r=0.55, P<0.0001 and r=0.35, P<0.0001, respectively), but not with maternal serum magnesium concentrations (r=0.004, P=0.98 and r=0.14, P=0.21). However, there was no correlation between the maternal and neonatal serum magnesium concentrations (r=0.10, P=0.15).

Conclusion: Total dose of magnesium infused to the mother correlates with neonatal serum magnesium concentrations. To keep neonatal serum magnesium concentrations within a range that is effective for neuroprotection and safe for the neonates, the total dose received by the mother needs to be monitored and limited.
Melt grafting copolymerization of glycidyl methacrylate onto acrylonitrile-butadiene-styrene (ABS) terpolymer
Authors: Shao Huiju , Qin Shuhao , Guo Jianbing , Wu Bin 
Published: March 2014

AbstractThe grafting copolymerization of glycidyl methacrylate (GMA) onto acrylonitrile-butadiene-styrene (ABS) terpolymer was carried out using the melt grafting process. Infrared (IR) spectra,
Pediatric Data Sharing in Genomic Research: Attitudes and Preferences of Parents
Authors: Burstein Matthew D. , Robinson Jill Oliver , Hilsenbeck Susan G. , McGuire Amy L. , Lau Ching C. 
Published: March 2014

OBJECTIVE:
In the United States, data from federally funded genomics studies are stored in national databases, which may be accessible to anyone online (public release) or only to qualified researchers (restricted release). The availability of such data exposes participants to privacy risk and limits the ability to withdraw from research. This exposure is especially challenging for pediatric participants, who are enrolled in studies with parental permission. The current study examines genomic research participants’ attitudes to explore differences in data sharing (DS) preferences between parents of pediatric patients and adult patients.

METHODS:
A total of 113 parents of pediatric patients and 196 adult participants from 6 genomics studies were randomly assigned to 3 experimental consent forms. Participants were invited to a follow-up structured interview exploring DS preferences, study understanding, and attitudes. Descriptive analyses and regression models were built on responses.

RESULTS:
Most parents (73.5%) and adult participants (90.3%) ultimately consented to broad public release. However, parents were significantly more restrictive in their data release decisions, not because of understanding or perceived benefits of participation but rather autonomy and control. Parents want to be more involved in the decision about DS and are significantly more concerned than adult participants about unknown future risks.

CONCLUSIONS:
Parents have the same altruistic motivations and grasp of genomics studies as adult participants. However, they are more concerned about future risks to their child, which probably motivates them to choose more restrictive DS options, but only when such options are made available.