bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYCOBACTERIUM MARINUM INFECTION OF THE SKIN IN JAPAN
Authors: Arai Hiroko , Nakajima Hiroshi , Nagai Ryukichi 
Published: April 2015

ABSTRACTBased on several reviews in Japanese dermatological journals, seventy‐five cases of bacteriologically confirmed Mycobacterium marinum infection of the skin from 1969 through 1980 have been accumulated. These 75 cases accounted for 82% of all reported cases of atypical mycobacterial infections of the skin in Japan (1).The following features were noted: Approximately half of the cases were occupationally related to fish handling; the other half occurred among fish tank hobby enthusiasts. The clinical presentation usually suggested sporotrichosis, with the majority of the skin eruptions appearing on the hand and 96 % being unilateral. Minocycline is recommended as the drug of choice.
COMPARISON OF PREKERATIN AND KERATIN MOLECULES IN HUMAN PLANTAR EPIDERMIS
Authors: Katagata Yohtaro , Aso Kazuo 
Published: April 2015

ABSTRACTKeratin and prekeratin were extracted from human plantar epidermis and carried on SDS‐PAGE. Prekeratin in the basal as well as spinous cell layer of the epidermis was composed of 6–7 bands (M.W.: about 71,000‐49,500), while keratin in the surface of stratum corneum was composed of 3–4 bands (M.W.: about 66,500–53,000), indicating molecular modulation of prekeratin during epidermal cell differentiation.Amino acid compositions of prekeratin and keratin were similar, glycine, glutamic acid and serine being major three amino acids. Since the keratin molecule is known to be composed of a repeating amino acid sequence [Fuchs & Green: Cell, 15, 887–893 (1978)], our result indicates that the modulation of molecular weight of prekeratin during differentiation might be due to enzymatic splitting of peptides of these structures.
EPIDERMAL NUCLEAR IMMUNOFLUORESCENCE
Authors: Kent Lawrence T. , Hu Chung‐Hong , Michel Beno , Tan Eng M. , Moskowitz Roland W. 
Published: April 2015

ABSTRACTIn sixteen patients, the relationship of epidermal nuclear immunofluorescence to the spectrum of connective tissue diseases and to serum antibodies was investigated. The clinical diagnoses were mixed connective tissue disease (7 patients), systemic lupus erythematosus (5 patients), scleroderma (3 patients) and rheumatoid arthritis (1 patient). Detailed serologic evaluation in 13 of the 16 patients revealed positive rheumatoid factor (7 patients), antinuclear antibody (13 patients), anti‐DNA (2 patients), anti‐n‐RNP (11 patients), anti‐Sm (2 patients), anti‐SS‐A (3 patients) and anti‐RANA (5 patients). Anti‐SS‐B and anti‐Scl‐70 were absent in all. Epidermal nuclear immunofluorescence on skin biopsy correlated with serum anti‐n‐RNP and is a useful marker for mixed connective tissue disease. However, it did not appear to serve as a specific marker for any one connective tissue disease. We discuss the mechanism(s) for this immunofluorescent pattern and believe it is an in vivo phenomenon.
INTRANUCLEAR TONOFILAMENTS IN A CASE OF GIANT CONDYLOMATA ACUMINATA (BUSCHKE AND LOEWENSTEIN)
Authors: Hori Makoto , Hirose Ryoji , Fujita Kazuo , Toriyama Fumi , Nishimoto Katsutaro , Yoshida Hikotaro 
Published: April 2015

ABSTRACTIntranuclear filamentous inclusions were seen electron microscopically in a small number of cells of the granular layer in a patient with giant condylomata acuminata. These inclusions were identified as cytoplasmic tonofilaments in a keratinocyte by their electron‐dense bundles composed of fine fibers which measured about 60 angstroms in diameter, and by the lack of periodicity in these fibers.As far as we know, such inclusions have been reported in only three cases, including the present case.The authors suggest that the appearence of intranuclear inclusions are indicative of viral verruca cells turning to rapid proliferation and abnormal mitosis.
SUCCESSFUL TREATMENT OF ALOPECIA AREATA WITH DINITROCHLOROBENZENE IN A PATIENT WITH DOWN'S SYNDROME
Authors: Hatamochi Atsushi , Ueki Hiroaki 
Published: April 2015

ABSTRACTDNCB treatment was carried out in a patient with Down's syndrome suffering from alopecia uninversalis. The patient showed excellent regrowth of hair on the treated sites. DNCB treatment is considered to be an alternative for persistent refractory alopecia areata in patients with Down's syndrome.
A CASE OF MCTD WITH URTICARIA‐LIKE LESIONS
Authors: Shishiba Takako , Takehara Kazuhiko , Hasegawa Setsuo 
Published: April 2015

ABSTRACTA 39‐year‐old woman with MCTD developed urticaria‐like lesions on the arms, showing leuko‐cytoclastic angiitis histologically.
A BASAL CELL EPITHELIOMA ON ANOTHER BASAL CELL EPITHELIOMA
Authors: Kikuchi Ichiro , Ogata Katsumi , Tajiri Akihiko 
Published: April 2015

ABSTRACTA basal cell epithelioma (BCE) apparently developed on another BCE, a hitherto undescribed condition to our knowledge. Fifteen specimens were prepared at representative planes, but there were no connections between the two tumors. In order to indirectly verify that there were two different tumors, the speed of growth was calculated; it roughly agreed with the calculation by Ono's formula, attesting to the diagnosis of BCE.
STATISTICS OF NEVI IN 3‐YEAR‐OLD JAPANESE CHILDREN
Authors: Kikuchi Ichiro 
Published: April 2015

ABSTRACTA total of 1319 3‐year‐old Japanese children were examined for nevi. Moles (small nevocellular nevi) were found in 40% of those examined; blue nevi could not be found at this age. Common Mongolian spots (Mongolian spots over the back and buttocks) were found in 94.8% and aberrant Mongolian spots (Mongolian spots appearing in other areas) in 4.4%. Brown Mongolian spots, a phenomenon appearing in some of those in the disappearing phase of Mongolian spots, appeared in 15.8%. Salmon patches on the eyelids and forehead had disappeared by age 3, but some regressing strawberry marks could be observed at this age.
EFFECTS OF SERUM ALBUMIN ON COAGULASE RELEASE FROM STAPHYLOCOCCUS AUREUS DERIVED FROM BULLOUS IMPETIGO
Authors: Dekio Satoshi , Jidoi Joji 
Published: April 2015

ABSTRACTThree strains of Staphylococcus aureus derived from bullous impetigo were cultured in media either not containing or containing serum albumin. The coagulase titers in each medium were measured as a function of the cultivation time. In the early‐mid phase of growth, the coagulase titers were increased to the same degree in both media. After that, the coagulase titers were maintained at the high level in the medium containing serum albumin but decreased in the medium not containing serum albumin. It is proposed that Staphylococcus aureus strains capable of causing bullous impetigo infect the injured skin surface with accumulation of plasma‐like material, and then grow with continuous release of coagulase. Under the protection of the fibrin membranes formed by the coagulase, the bacteria are established, and then the bullous lesions are formed.
COMPARISON OF THE IN VITRO ANTIFUNGAL ACTIVITIES OF CLOTRIMAZOLE, MICONAZOLE, ECONAZOLE AND EXALAMIDE AGAINST CLINICAL ISOLATES OF DERMATOPHYTES
Authors: Kusunoki Toshio , Harada Seiichi 
Published: April 2015

ABSTRACTThe in vitro antifungal activities of clotrimazole, miconazole nitrate, econazole nitrate, and exalamide against 64 clinical isolates of various dermatophyte species obtained from out‐patients seen at the Nippon Medical School Hospital were simultaneously compared using a liquid microculture method. The rank order of activity against the 64 isolates of dermatophytes was clotrimazole > econazole nitrate > miconazole nitrate > exalamide. For a given antimycotic, the minimal inhibitory concentration was affected by the dermatophyte species.
ELECTRON MICROSCOPIC OBSERVATION OF DYSKERATOTIC CELLS IN ACQUIRED ZINC DEFICIENCY
Authors: Hanada Katsumi , Hada Tomoko , Hashimoto Shizuo Satoh Isao , Katabira Yasuo 
Published: April 2015

ABSTRACTThis paper describes the electron microscopic structure of dyskeratotic cells observed in cutaneous lesions of 3 cases with acquired zinc deficiency. Double‐zoned cytoplasm consisted of amorphous cell organelles and irregular tonofilaments occasionally containing lipid droplets and melanosomes. Small nuclei were seen in many dyskeratotic cells around the blister. Various degrees of alteration were noted in cells in erythematous lesions following the disappearance of all desmosomes. The halo formation of dyskeratotic cells with double‐zoned cytoplasm indicates an alteration in keratinocytes, which may be caused by superoxide radical anions resulting from inactivity of superoxide dismutase, a zinc‐dependent enzyme.
ESTABLISHMENT OF CELL LINES FROM HUMAN MALIGNANT MELANOMAS AND THEIR APPLICATION TO THE ASSESSMENT OF NATURAL KILLER‐LIKE CYTOTOXICITY
Authors: Kondo Shigeo , Sato Norio , Sato Kumi , Hozumi Yutaka , Aso Kazuo 
Published: April 2015

ABSTRACTTwo melanoma cell lines (HMY‐1 and HMY‐2) derived from two patients suffering from malignant melanomas have been established in vitro.A study of cellular immunity was conducted, with particular attention to natural killer cell cytotoxicity mediated in vitro in one patient with melanoma (HMY‐2) using the established cell lines. During the last stages of the clinical course of the metastasized HMY‐2 patient, both autologous and allogeneic natural killer‐like cytotoxicities of her lymphocytes weakened drastically. The natural killer‐like cytotoxicity of all autologous and allogeneic lymphocytes was enhanced after their preincubation for 3 days in the presence of BCG, PPD or PHA.
THE ROLE OF POLYMORPHONUCLEAR CELLS IN PSORIASIS
Authors: Tosca A. , Varelzidis A. , Kavadda H. , Michalopoulos M. , Hatzis J. , Stratigos J. 
Published: April 2015

ABSTRACTNeutrophil cell function in psoriasis was studied through polymorphonuclear cell adherence to nylon wool, chemotaxis under agarose, and phagocytosis ofCandida albicans. Differences in PMN adherence were not found between psoriatics and controls. The chemotactic response to zymosan activated serum (ZAS) did not differ between psoriatic and normal PMNs. The psoriatic serum exhibited chemoattracting properties equal to ZAS. Psoriatic serum was shown to be chemoattracting to psoriatic and normal PMNs whereas normal serum was only to psoriatic cells. Differences in recognition mechanisms between psoriatic and normal cells are proposed. Phagocytosis ofCandidaby psoriatic PMNS was earlier and more prolonged and candidacidal activity was earlier and definetely increased at given times compared to normal PMNs. A protective role of psoriatic PMNs in Munro's microabcesses against bacterial invasion is postulated.
GUINEA PIG ENDOTHELIAL CELLS IN CULTURE
Authors: Masuzawa Mikio , Suzuki Yuhsuke , Nishiyama Shigeo , Nishioka Kiyoshi , Ryan Terence J. 
Published: April 2015

ABSTRACTIn pursuit of a model for the studies of endothelial cells in a readily available experimental animal, culture methods and the in vitro characteristics of endothelial cells isolated from guinea pig aorta are described. Endothelial cells were harvested by trypsinization with perfusion techniques and cultured in Eagle's minimal essential medium supplemented with 10% fetal calf serum. Their passage cultivation was possible for more than six months and they have maintained the in vitro morphological characteristics of endothelial cells.The cell doubling time of the passaged endothelial cells was 115–131 hours. The growth of guinea pig aorta endothelial cells was satisfactory in Eagle's minimal essential medium containing guinea pig serum and fetal calf serum, but not horse and calf serum. Of the various concentrations of fetal calf serum in Eagle's minimal essential medium, 3% fetal calf serum did not activate cell growth and 10% fetal calf serum induced the best growth; in 20% concentration of fetal calf serum the cell growth rate decreased as compared with the rate in 10% fetal calf serum. DNA synthesis (3H‐thymidine uptake) of the cells in 20% fetal calf serum was less than that in 10% fetal calf serum. These results have no relation to cell generation nor to the cell density. With respect to the type of media supplemented with 10% fetal calf serum, Dulbecco's modified Eagle's medium, RPMI −1640 and Eagle's minimal essential medium were satisfactory for the cultivation of guinea pig aorta endothelial cells, but Medium‐199 was unsuitable. By adding endothelial cell growth supplement (75, 150 mcg) or fibroblast growth factor (100, 200 ng) to Eagle's minimal essential medium containing 3% fetal calf serum, the cell growth was stimulated approximately three times. Fibronectin‐coated wells did not activate cell growth.
PSORIASIS AMONG ARABS
Authors: Kanwar A.J. , Belhaj M.S. , Bharija S.C. , Malhotra Y.K. 
Published: April 2015

ABSTRACTPsoriasis constituted 1.46% of all the dermatological disorders seen during the one year period of the study. Males were slightly more frequently affected than females and most of the patients were in their 3rd and 4th decade. The mean age of males (36.95) was 7 years higher than that of females (29.83). Familial occurrence was recorded in 14.39% of the cases. Itching appeared to be a prominent feature; only 15.62% quoted worsening in colder months. Extensor surfaces were the commonest sites of involvement. More than one third of the cases showed nail changes of one type or the other. Psoriatic arthropathy and pustular psoriasis were rather rare.
DRUGS CAUSING FIXED ERUPTIONS
Authors: Kanwar A.J. , Belhaj M.S. , Bharija S.C. , Mohammed Mohgat 
Published: April 2015

ABSTRACTSeventy one patients having fixed drug eruptions were subjected to provocation tests. The greatest number of cases were due to acetylsalicylic acid (18). Other drugs responsible were hyposcine butylbromide (15), oxyphenbutazone (14), sulphadiazine (7), tetracycline hydrochloride (6), metamizole (5) and ibuprofen (3). Other drugs were less frequent. There was evidence of cross sensitivity between various tetracyclines and sulphonamides and also between oxyphenbutazone and phenylbutazone. One patient had two groups of lesions, each reacting to a different, chemically unrelated drug.
REDUCTION OF CHEMOTACTIC ACTIVITIES IN PSORIATIC LESIONS BY HYPERTHERMIA TREATMENTS
Authors: Isoda Midori , Oosaki Mitsuhiko , Yasumoto Shinichiro , Urabe Harukuni 
Published: April 2015

ABSTRACTThe effect of the local heat treatment on the chemotactic activity in psoriatic scales was studied by using a modified Boyden chamber method. Hyperthermic treatments were given to psoriasis plaques and scales were taken from these plaques before and after treatment. The psoriasis improved over a two week period of these treatments, but plaques were still present and scaling at that time. Treatment with local hyperthermia significantly reduced the activity for polymorphonuclear leukocytes in psoriatic scales (p<0.05).This result suggests that the inhibition of chemotactic activity may be involved in the action mechanism of hyperthermia in psoriatic lesions.
MONOSACCHARIDE IN HIGH FRUCTOSE SYRUP AS AN ETIOLOGICAL FACTOR OF URTICARIA
Authors: Nishioka Kiyoshi , Katayama Ichiro , Sano Shigeharu , Numata Tsunemi , Yamamoto Shoso 
Published: April 2015

ABSTRACTA 30 year‐old man developed urticaria by taking a refreshing drink. Two of its ingredients, invert sugar and high fructose syrup (GF sugar), induced urticarial attacks. GF sugar was analyzed by thin layer chromatography. A by‐product monosaccharide, psicose, was suspected as the responsible factor. Psicose‐rich sugar of Lobry de Bruyn transformation was prepared and skin‐tested. It and its psicose containing fraction of thin layer chromatography induced positive skin reactions. Furthermore, GF sugar released histamine from leukocytes presensitized with the patient's serum. It is therefore concluded that the urticarial reaction observed in this patient was mediated by Type I hypersensitivity against psicose.
LUPOID SYCOSIS WITH EXTENSIVE MUTILATIONS (AN INSTANCE OF THERAPEUTIC HELPLESSNESS)
Authors: Reddy B.S.N. , Selvaraju M. , Krishnaram A.S. , Garg B.R. 
Published: April 2015

ABSTRACTA case of lupoid sycosis occurring in an adult male is reported as an interesting entity because of its early onset, extensive involvement, rarity of the disease and paucity of literature. Therapeutic helplessness became evident when treatment with adequate appropriate antibiotics, steroid‐antibiotic combinations, and other empirical lines of treatment resulted in little satisfactory improvement. The disease is still persisting with areas of active lesions and widespread, mutilated, burnt‐out patches of depigmented atrophic and hypertrophic scars, cicatricial alopecia, ectropion, mild flexion deformity of the knees, personality changes, etc. Therapeutic suggestions are invited.
THE ANERGY OF ANNULARITY
Authors: Lobitz Walter C. , White Clifton R. , Cooper Kevin D. 
Published: April 2015

ABSTRACTWe explain the types and historical studies of annular and arciform lesions. In addition, we present a study of annular lesions on a patient with Erythema Annulare Centrifugum (EAC), proven to be caused by a delayed type hypersensitivity (DTH) to trichophyton antigen, using monoclonal antibodies to identify subsets of tissue monocytes outside the EAC lesion, in the inflamed border of the same EAC lesion and inside the circle (anergic area) of the same EAC lesion. Macrophages (M), Langerhans' cells (LC), pan‐T cells, and the T cell subsets of helper/inducer T cells, suppressor/cytotoxic T cells, activated T cells and Ia (HLA‐immune associated) T cells were identified, localized, and compared in the respective areas of the annular lesion.An adequate number of antigen presentor cells were present in the dermis (and epidermis) in all areas. Langerhans cells (LC) were outside the inflamed border. Macrophages were in the border and inside the circle. Also, there were an adequate number of antigen receptor cells in all areas; pan‐T cells were abundant outside, in the border and inside the circle. Although there were remarkably fewer helper and suppressor T cells inside the circle, the ratio of helper to suppressor T cells remained the same and unchanged in all the areas. None of the above could explain the anergy of EAC annularity.The Ia T cells were abundant in the epidermis and dermis outside the inflamed border and in the inflamed border. However, they were completely absent inside the circle. There were no Ia T cells inside of the EAC inflamed border even though the total number of T cells were abundant in that area. Apparently, the T cells had temporarily lost (4–6 weeks) their HLA histocompatibility inside of the annular and arciform EAC lesions. Having done so, these T cells could no longer receive antigen from M and Lc, could not become activated and thus, could not produce this DTH (Type IV) reaction. This defect may explain the anergy of DTH annularity.
KIMURA'S DISEASE
Authors: Sawada Yukimasa , Nomura Kazuo 
Published: April 2015

ABSTRACTSubcutaneous masses removed from periauricular lesions showed histologic features of Kimura's disease. These consist of remarkable vascular proliferation and lymphoid follicles in the subcutis rather than lower dermis, with numerous infiltrations of tissue eosinophils. Moderate eosinophilia and high IgE levels in the peripheral blood, and positive intradermal test and RAST with candida antigen were observed, which are also characteristic of Kimura's disease.However, the patient is a middle‐aged female without any swollen lymph nodes or systemic involvement. Moreover, a few vessels with prompt endothelial cells were recognized histologically. This resembles subcutaneous angiolymphoid hyperplasia with eosinophilia and its allied diseases.Review of the relevant literature and study of our own case suggested that Kimura's disease is highly similar to subcutaneous angiolymphoid hyperplasia with eosinophilia and its related diseases. We surmise that Kimura's disease belongs to this group.
STATUS OF FIBRIN DEGRADATION PRODUCTS IN LEPROSY
Authors: Saha Kunal , Agarwal Satish Kumar , Sehgal Virender Nath 
Published: April 2015

ABSTRACTPlasmafibrinogen and fibrin degradation products (FDP) were estimated in 45 control subjects and 45 patients with various types of leprosy including 16 patients with erythema nodosum leprosum (ENL). The levels of FDP were quantified by using a suspension ofStaphylococcus aureus(Newmans D2C strain). Significantly higher levels of plasma fibrinogen and FDP were observed in patients with lepromatous leprosy, especially with ENL, than in the controls. Only 3 lepromatous patients had plasma fibrinogen levels below the normal range; 2 of them had severe ENL and were suffering from nasal bleeding. Levels of D and E fragments were also estimated in another 20 control subjects and 13 lepromatous patients by haemagglutination inhibition technique and were found to be significantly elevated in the patients. It was inferred that raised FDP levels in such patients indicated an ongoing occult fibrinolysis during lepra reaction. A few lepromatous patients may show hypofibrinogenomia during severe ENL episodes.
A CASE OF CUTANEOUS PLASMACYTOSIS
Authors: Ishii Norihisa , Hayashi Masayuki , Nakajima Hiroshi , Nagai Ryukichi 
Published: April 2015

ABSTRACTA sixty‐six‐year‐old woman has had numerous macules all over her body for a year without any systemic involvements. Plasma cells had infiltrated into the dermis and perivascular region, but showed no mitotic figures nor atypicalities. Laboratory examinations for Bence Jones protein, serum gammaglobulin, and immunoglobulins demonstrated no abnormal findings. The surface markers of the plasma cells were polyclonal; they were stained by anti IgA, IgG, IgM, kappa, and lambda. We consider this case not to be one of a true tumor but one of rather benign reactive proliferation of plasma cells in the skin, although its etiology is unclear.
T Cell Receptor Expression by Dendritic Epidermal T Cells
Authors: Shimada Shinji 
Published: April 2015

AbstractDendritic epidermal T cells (DETC) in murine epidermis express the γδ T cell receptor (TCR). The major population of DETC utilize Vγ5 and Vδ1 without any junctional diversity, corresponding to the earliest fetal thymocytes which express TCRγδ. Using PCR, we recently found another population of DETC which express VγI‐Vδ6 with junctional diversity in addition to Vγ5‐Vδ1, although they exist in small numbers in normal mice. In athymic nude mice, Vγ1+ cells also exist. Therefore, this subset of γδ T cells is the product of an extrathymic pathway. These Vγ1 + cells may recognize mycobacterium antigen or heat shock protein (HSP), thus playing an important role in the first defense of the skin.In contrast to normal mice, in nude mice, we could not detect any DETC using anti CD3ε antibody (2C11). In order to solve this puzzle, we examined the components of TCR complex utilizing immunoprecipitation and northern blot analysis. TCR is composed of either αβ or γδ chains associated with CD3γ, δ, ε and ζ‐ζ chains. By immunoprecipitation of 125I labelled DETC cell lines using anti‐CD3ε antibody, we detected γδ chains and CD3γ and CD3ε chains, but not CD3δ or CD3ζ chains. Northern blot analysis showed that these cells express CD3γ, ε, and ζ chains, but not the CD3δ chain. We concluded that the lack of the CD3δ chain and/or CD3ζ protein may change the configuration of the TCR complex, leading to the lack of staining by anti‐CD3ε antibody in the DETC of nude mice. This defect of TCR in nude DETC may explain functional abnormalities such as poor response to mitogens and diminished killing activity. Moreover, in ζ‐chain deficient mice, full expression of TCR was observed. This fact suggested that DETC may utilize the FcεRIγ chain instead of the ζ chain for cell surface expression or signal transduction of TCR.