bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective C‐Methylenation of N‐Unsubstituted Indoles Using CO2 Under NaBH4/I2 System
Authors: Guo Zhiqiang , Wu Jinglong , Wei Xuehong , Xi Chanjuan 
Published: August 2024

AbstractThe selective C‐methylenation of N‐unsubstituted indoles using CO2 as the C1 source to access diindolylmethane (DIM) and its derivatives is described. This reaction provides a novel method for four‐electron reductive functionalization of CO2 with N‐unsubstituted indoles via formation of C−CH2−C bonds, and a new access to molecular structures.
Mutation pressure mediates a pattern of substitution rates with latitude and climate in carnivores
Authors: Zhao Chao , Liu Guangshuai , Yang Xiufeng , Wang Xibao , Zhou Shengyang , Liu Zhao , Liu Kangning , Zhang Honghai 
Published: August 2024

AbstractThe evolutionary patterns of the mitochondrial genome are influenced by both adaptive and nonadaptive forces, with their contributions varying among taxa. There appears to be a correlation linking mutagenesis and latitude, which could be due to differences in metabolic rates. These discrepancies in metabolic rates exhibit a positive connection with mutation pressure. On this basis, we hypothesise that nonadaptive forces play a role in the differences in mutation rates observed along latitudinal gradients. In this study, we selected widely distributed carnivores as representatives of mammals to test our hypothesis. We examined the correlations between the dN/dS ratio (ω), as well as the substitution rates (dS and dN), of 13 PCGs in the mtDNA of 122 carnivores, and the latitude and climatic factors. We found that taxa distributed in higher latitudes tend to have higher substitution rates, but not ω values indicating selective pressure. Notably, dN shows a strong positive correlation with dS, although dS is primarily influenced by mutation pressure, while dN is also influenced by effective population size (Ne). Phylogenetic generalised least squares (PGLS) regression analyses showed that both substitution rates were correlated with climatic factors representing the temperature, precipitation and variability of climate. Based on our findings, we propose that the mutations are primarily influenced by nonadaptive forces (mutation pressure). This forms the fundamental premise for natural selection and speciation. Moreover, the correlation between substitution rates and latitudinal distribution and climate, which are outcomes of nonadaptive factors, can aid in comprehending the global distribution of species diversity.
Internal transcribed spacer as effective molecular marker for the detection of natural hybridization between the bivalves Pinna nobilis and Pinna rudis
Authors: Catanese Gaetano , Vázquez‐Luis Maite , Giacobbe Salvatore , García‐March José Rafael , Zotou Maria , Patricia Prado , Papadakis Orestis , Tena‐Medialdea José , Katsanevakis Stelios , Grau Amalia 
Published: August 2024

AbstractThe Pinna nobilis, a Mediterranean mollusc, has suffered population declines due to a massive mortality event associated with various factors including the parasite Haplosporidium pinnae. Some populations show resilience, possibly due to local environmental conditions. In this study, a molecular multiplex PCR method was developed using species‐specific primers targeting Internal Transcribed Spacer (ITS) regions of P. nobilis and P. rudis, allowing accurate species identification and hybrid detection. Samples from Mediterranean areas were analysed, including putative hybrids and individuals from five other bivalve species. DNA was isolated, ITS regions were amplified and sequenced, and phylogenetic analyses confirmed species differentiation and primer specificity. The multiplex‐PCR successfully identified P. nobilis, P. rudis, and their hybrids based on distinct amplicon patterns. This study highlights the value of molecular tools in species conservation, especially for monitoring and managing hybridization, supporting effective biodiversity conservation strategies.
Synthesis and Anticancer Properties of Heterobimetallic Fe(II)/Pd(II) Complexes Bearing Different Butadienyl Fragments
Authors: Bortolamiol Enrica , Dalla Pozza Maria , Demitri Nicola , Rizzolio Flavio , Visentin Fabiano , Gasser Gilles , Scattolin Thomas 
Published: August 2024

AbstractIn this work, we describe the synthesis of heterobimetallic Fe(II)/Pd(II) complexes bearing different halides and halide‐substituted butadienyl ligands. The synthetic approach involves the preparation of suitable Pd(II)‐butadienyl precursors containing a thioquinoline moiety, followed by the substitution of this relatively labile ligand with dppf (dppf=1,1′‐bis(diphenylphosphino)ferrocene). With the exception of complexes containing at least one iodine atom, all compounds were synthesized in high yields and purity, and thoroughly characterized using spectroscopic and diffractometric methods. The investigation of the antiproliferative activity of the synthesized organometallic complexes against ovarian and breast cancer cell lines revealed that all compounds exhibit noteworthy cytotoxicity, with IC50 values in the micromolar range and generally comparable to those of cisplatin. Interestingly, significant differences of cytotoxicity among the analysed compounds were observed in the case of MRC‐5 normal cells. Of particular interest is the dichloride derivative 3 a, which is essentially inactive towards non‐cancerous cells (IC50>100 μM), thus demonstrating promising in vitro selectivity that we believe warrants further investigation in the future.
An Electrochemical Sensor Based on 3D Graphene‐Cyclodextrin Nanohybrid for Enhanced Sensitivity Detection of Nitroaromatic Compounds
Authors: Di Ziao , Zhang Yu , Ding Zuhang , Huang Jiayu , Mao Lujia , Wei Hongjun , Zhao Jin 
Published: August 2024

AbstractThis study presents an electrochemical sensor for sensitive detection of nitroaromatic compounds using a three‐dimensional (3D) porous graphene with polyoxypropylene supported reduced graphene oxide (PEA‐RGO) and embedded β‐cyclodextrins (β‐CD). First, PEA‐RGO was synthesized by the condensation reaction between graphene oxide (GO) and amino‐terminated polyoxypropylene (PEA), followed by hydrazine hydrate reduction. Then, β‐CD was embedded into the porous of the above 3D graphene to obtain PEA‐RGO/β‐CD nanohybrid. Various characterization techniques, such as microscopy imaging, infrared spectrometry, thermal analysis and electrochemical measurements, were employed to confirm the successful synthesis and unique 3D architecture structure of PEA‐RGO/β‐CD. Afterwards, the glassy carbon electrode (GCE) was modified by PEA‐RGO/β‐CD, and its electrochemical detection performances were investigated for detecting nitroaromatic compounds, including nitrobenzene (NB), 2‐nitroaniline (2‐NA), 2‐chloronitrobenzene (2‐NCB), and 2‐nitrophenol (2‐NP). The results of electrochemical measurements exhibited relative higher sensitivities (1.88 μA/μM/cm2 for NB, 2.13 μA/μM/cm2 for 2‐NA, 2.35 μA/μM/cm2 for 2‐NP, 0.83 μA/μM/cm2 for 2‐NCB, respectively) and lower limits of detection (LOD) (39.4 nM for NB, 44.6 nM for 2‐NA, 75.6 nM for 2‐NP, 69.6 nM for 2‐NCB, respectively). Additionally, the proposed sensor was also applied in detection in real samples with recovery range of 88.13%–109.52 %. The enhanced detecting performances can be attributed to the synergistic effect of the high conductivity from RGO, recognition capabilities from β‐CD, and excellent properties from 3D structure. The present work would provide a robust tool for environmental monitoring and the detection of nitroaromatic pollutants.
All‐body concept and quantified limits of cooperativity and related effects in homodromic cyclic water clusters from a molecular‐wide and electron density‐based approach
Authors: Cukrowski Ignacy , Zaaiman Stéfan , Hussain Shahnawaz , de Lange Jurgens H. 
Published: August 2024

AbstractWe strongly advocate distinguishing cooperativity from cooperativity‐induced effects. From the MOWeD‐based approach, the origin of all‐body cooperativity is synonymous with physics‐ and quantum‐based processes of electron (e) delocalization throughout water clusters. To this effect, over 10 atom‐pairs contribute to the total e‐density at a BCP(H,O) between water molecules in a tetramer. Intermolecular all‐body e‐delocalization, that is, cooperativity, is an energy‐minimizing process that fully explains non‐additive increase in stability of a water molecule in clusters with an increase in their size. A non‐linear change in cooperativity and cooperativity‐induced effects, such as (i) structural (e.g., a change in d(O,O)) or topological intra‐ and intermolecular properties in water clusters (e.g., electron density or potential energy density at bond critical points) is theoretically reproduced by the proposed expression. It predicted the limiting value of delocalized electrons by a H2O molecule in homodromic cyclic clusters to be 1.58e. O‐atoms provide the vast majority of electrons that “travel throughout a cluster predominantly on a privileged exchange quantum density highway” (⋅⋅⋅O–H⋅⋅⋅O–H⋅⋅⋅O–H⋅⋅⋅) using Bader's classical bond paths as density bridges linking water molecules. There are, however, additional electron exchange channels that are not seen on molecular graphs as bond paths. A 3D visual representation of the “privileged” and “additional” exchange channels as well as detailed intra‐ and inter‐molecular patterns of e‐sharing and (de)localizing is presented. The energy stabilizing contribution made by three O‐atoms of neighboring water molecules was found to be large (−597 kcal/mol in cyclic hexamer) and 5 times more significant than that of a classical O–H⋅⋅⋅O intermolecular H‐bond.
Impact of oxytosis on the cross‐talk of mTORC with mitochondrial proteins in drug‐resistant cancer stem cells
Authors: Pandrangi Santhi L. , Chittineedi Prasanthi , Manthari Ram K. , Suhruth Balaji 
Published: August 2024

AbstractBy delivering the environmental inputs to transport nutrients and growth factors, Mechanistic Target of Rapamycin (mTOR) plays a significant role in the growth and metabolism of eukaryotic cells through the regulation of numerous elementary cellular processes such as autophagy, protein synthesis, via translation of mitochondrial protein transcription factor A mitochondrial, mitochondrial ribosomal proteins, and mitochondrial respiratory complexes I &V that are encoded in the nucleus with the help of translation initiation factor 4E–BP. These mitochondrial proteins are involved in cell signaling to regulate proper cell growth, proliferation, and death which are essential for tumor growth and proliferation. This suggests that tumor cells are dependent on mTORC1 for various metabolic pathways. However, this crucial regulator is activated and regulated by calcium homeostasis. Mounting evidence suggests the role of calcium ions in regulating mitochondrial enzymes and proteins. Hence, disrupting calcium homeostasis leads to calcium‐dependent cell death called “Oxytosis” through hampering the expression of various mitochondrial proteins. “Oxytosis” is a novel non‐apoptotic cell death characterized by glutamate cytotoxicity and ferritin degradation. The present review focuses on the crosstalk between mTORC1 and mitochondrial proteins in the cancer pathophysiology and the impact of calcium ions on disrupting mTORC1 leading to the induction of “Oxytosis.”
Anatomical biliary reconstruction as an ultimum refugium for selective cases—History and current state of knowledge
Authors: Sevcik Jan , Massaro Maria Stefania , Palek Richard , Moulisova Vladimira , Liska Vaclav 
Published: August 2024

AbstractReconstruction of extrahepatic bile ducts is a staple procedure of HPB surgery. The current standard for most cases is a nonanatomical bilioenteric reconstruction, a satisfactory option for the majority of patients. However, it cannot be used for a small number of selective cases (short bowel syndrome, severe abdominal adhesions), where an anatomical reconstruction with or without an interponate can be used. This review summarizes current knowledge about tissue and material usage for experimental and clinical anatomical bile duct reconstruction in the last 100 years. A Pubmed database was searched for published articles about anatomical extrahepatic bile duct reconstruction in experimental and clinical settings ranging from 1920 to 2022. To date, the truly optimal interponate material has not yet been found. However, evidence reveals important properties of such material, most importantly its biodegradability and neovascularization in the recipient's body. The role of internal bile duct stenting for anatomical reconstruction seems important for the outcome. Anatomical reconstruction of extrahepatic bile ducts is an uncommon but usable technique in unique cases when a nonanatomical reconstruction cannot be done. The optimal properties of interponate material for anatomical bile duct reconstruction have been more clarified, although further research is required.
Parental and child's psychosocial and financial burden living with an inherited metabolic disease identified by newborn screening
Authors: Schnabel‐Besson Elena , Garbade Sven F. , Gleich Florian , Grünert Sarah C. , Krämer Johannes , Thimm Eva , Hennermann Julia B. , Freisinger Peter , Burgard Peter , Gramer Gwendolyn , Morath Marina A. , Tuncel A. Tunç , Keßler Svenja , Hoffmann Georg F. , Kölker Stefan , Mütze Ulrike 
Published: August 2024

AbstractNewborn screening (NBS) is one of the most effective measures of secondary prevention. While the benefit of NBS on the clinical long‐term outcomes of children with inherited metabolic diseases (IMD) has been demonstrated, the potential burden of families living with an early diagnosed and treated child with an IMD has not been thoroughly investigated. The aim of this longitudinal questionnaire‐based study on 369 families living with a child with an IMD was to investigate the psychosocial and financial burden following a true‐positive NBS. The reported psychosocial burden differed between children and their parents, and was associated with the child's age, diagnosis, and treatment. At younger ages, parent‐reported burden was higher for the parents than for the individual child, while it increased for children and decreased for parents as the child grew older. Furthermore, psychosocial burden increased if the child required a strict dietary treatment and was at risk of metabolic decompensation. Regardless of diagnosis and treatment, the developmental delay of their child independently increased the parental psychosocial burden. Financial burden was reported by 24% of all families, and was higher in low‐income families and in families whose children required dietary treatment. In conclusion, a substantial psychosocial and financial burden was revealed for children and their families after true‐positive NBS. Since this burden is likely to have a negative impact on the long‐term individual health benefits of NBS, this study underlines the importance of regularly assessing the psychosocial and financial needs of these families.
Effect of glycosylation, acylation and pyranylation at cyanidin C‐ring on its interaction with vitamin C in apple juice beverage matrix
Authors: Huang Jinping , Yang Chen , Pan Xin , Wu Jihong , Lao Fei 
Published: August 2024

AbstractBACKGROUNDSynchronous degradation between anthocyanin and vitamin C was found in fruit and vegetable juice matrices. To investigate whether the C‐ring of anthocyanin is the key site of this interaction, cyanidin with four different C‐ring modifications (3‐glucosylation, 3,5‐diglucosylation, 6″‐malonylation, pyranylation) was added to vitamin C‐containing apple juice, and the changes of anthocyanin retention, vitamin C retention, color, antioxidative activity and differential metabolites were analyzed.RESULTSThe anthocyanin retention was in the order of pyranylation >6″‐malonylation >3,5‐diglucosylation >3‐glucosylation. The vitamin C retention was in the order of 6″‐malonylation > pyranylation >3,5‐diglucosylation >3‐glucosylation. The order of color stability was the same as that of anthocyanin retention, and the order of antioxidative activity was opposite to that of vitamin C retention. The results showed that modification at the C‐ring limited the activity of anthocyanin, and suggested that the C‐ring was one of the key sites for anthocyanin and vitamin C interaction. The shared differential metabolite of all apple juice matrices added with different anthocyanins was trans‐hinokiresinol, which was likely generated from anthocyanin skeleton reacted with certain compounds in apple juice.CONCLUSIONThis study showed that modification of the anthocyanin C‐ring could affect the anthocyanin and vitamin C interaction to some extent, which provided valuable insights for the application of anthocyanin C‐ring modification in shelf‐life quality control of typical fruit and vegetable beverages with the coexistence of anthocyanin and vitamin C. © 2024 Society of Chemical Industry.
Deer oil improves ulcerative colitis induced by DSS in mice by regulating the intestinal microbiota and SCFAs metabolism and modulating NF‐κB and Nrf2 signaling pathways
Authors: Li Hongyan , Han Lu , Zong Ying , Feng Ruyi , Chen Weijia , Geng Jianan , Li Jianming , Zhao Yan , Wang Yuqi , He Zhongmei , Du Rui 
Published: August 2024

AbstractBACKGROUNDDeer oil (DO), a byproduct of deer meat processing, possesses high nutritional value. This study aims to evaluate the protective effects of DO on dextran sulfate sodium (DSS)‐induced ulcerative colitis (UC) in mice and to explore its potential mechanisms of action.RESULTSDO was found to inhibit weight loss and colon shortening in colitis mice, significantly reduce disease activity index scores, and notably enhance the levels of tight junction proteins in colon tissues, thus improving intestinal barrier function. ELISA results indicated that DO markedly alleviated the mice's oxidative stress and inflammatory responses. Western blot analysis further demonstrated that DO significantly inhibited the phosphorylation of NF‐κB while up‐regulating the expression levels of Nrf2 and HO‐1 proteins. Additionally, DO increased the abundance of beneficial bacteria such as Odoribacter, Blautia, and Muribaculum, reduced the abundance of harmful bacteria such as Bacteroides, Helicobacter, and Escherichia‐Shigella, and promoted the production of short‐chain fatty acids.CONCLUSIONOur study provides the first evidence that DO can effectively improve DSS‐induced UC in mice. The underlying mechanisms may involve maintaining intestinal barrier function, inhibiting inflammation, alleviating oxidative stress, and modulation of gut microbiota. These findings offer valuable insights for developing DO as an adjunct treatment for UC and as a functional food. © 2024 Society of Chemical Industry.
GLIM‐defined malnutrition in patients with acute abdomen associated with poor prognosis and increased economic burden: A cross‐sectional study
Authors: Ma Wei , Cai Bin , Li Hua‐xin , Tan Xin , Deng Meng‐jie , Jiang Li , Sun Ming‐wei , Jiang Hua 
Published: August 2024

AbstractBackgroundThe Global Leadership Initiative on Malnutrition (GLIM) criteria have rapidly developed into a principal methodological framework for nutrition diagnosis. However, the applicability of the GLIM criteria in patients with acute abdomen has not been validated.MethodsThis is a cross‐sectional study conducted on patients diagnosed with acute abdomen and admitted to a tertiary hospital in southwest China. Nutrition risk screening was conducted using the Nutrition Risk Screening 2002, and patients identified with nutrition risk were assessed for malnutrition based on the GLIM criteria.ResultsWe enrolled a total of 440 patients with acute abdomen. The top three diagnoses of acute abdomen were intestinal obstruction (47.2%), acute appendicitis (23.1%), and digestive system perforation (8.8%). The prevalence of nutrition risk was 46.5%, with a malnutrition rate of 32.5% based on the GLIM. Patients with malnutrition according to the GLIM showed significantly higher rates of intensive care unit (ICU) admission (13.28% vs 7.07%; P = 0.003), increased hospitalization costs (median: 3315USD [interquartile range (IQR): 978–7852] vs 1641 [IQR: 816–3523] USD; P < 0.001), and longer length of hospital stay (LOS) (median: 8 [IQR: 5–13] vs 6 [IQR: 4–8] days; P < 0.001) compared with patients without malnutrition. Multivariate analysis indicated that GLIM‐defined malnutrition was an independent predictor of hospitalization costs, and severe malnutrition was an independent predictor of ICU admission.ConclusionGLIM criteria are applicable for diagnosing malnutrition in patients with acute abdomen. The prevalence of malnutrition was high in patients with acute abdomen. Malnutrition was associated with increased ICU admission and LOS, along with higher economic burden.
Combination Tonsillectomy and Hypoglossal Nerve Stimulation for Sleep Apnea Patients With Oropharyngeal Lateral Wall Collapse
Authors: Huyett Phillip , Wellman Andrew , Caruso Victoria , Sumner Jeffrey , Aishah Atqiya , Azarbarzin Ali , Sands Scott , Vena Daniel 
Published: August 2024

AbstractObjectiveThe efficacy of hypoglossal nerve stimulation (HGNS) therapy is limited by obstruction of the oropharyngeal lateral walls (OLWs). Our objective was to investigate the effect of palatine tonsillectomy on HGNS efficacy in obstructive sleep apnea (OSA) patients with OLW collapse.Study DesignCase‐control study of patients with moderate‐to‐severe OSA, complete‐or‐partial OLW collapse, and small tonsils (1 − 2+). Concomitant palatine tonsillectomy and HGNS (HGNS+T) were compared against a control group of patients who underwent HGNS alone.SettingSingle academic institution.MethodsStudy outcomes were measures of HGNS efficacy defined as a %reduction in apnea‐hypopnea index (AHI) (primary) and successful treatment response (50% AHI reduction to <15/h, logistic regression), respectively. Regression analyses quantified the additional effect of tonsillectomy (HGNS+T vs HGNS alone, independent variable) on HGNS efficacy. Analyses were adjusted for OLW collapse severity (complete vs partial), tonsil size, age, sex, body mass index, and baseline AHI.ResultsNineteen patients underwent HGNS+T and had follow‐up sleep testing for the current analysis. The control group (HGNS alone) consisted of 78 patients. Baseline demographics and OSA severity were similar between the groups, except HGNS+T group had increased prevalence of complete OLW collapse. Linear regression demonstrated that adding tonsillectomy resulted in an additional 22.9% [7.5, 35.2] reduction in AHI [95% confidence interval, CI] (P = .006), and 8.6 [1.7,43.4] (P = .010) greater odds [95% CI] of a successful treatment response with HGNS.ConclusionCompared to historically poorer outcomes of HGNS in patients with OLW collapse, these early results suggest combining tonsillectomy with HGNS may represent a promising strategy to improve success rates.