bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence and Distribution of Plant Parasitic Nematodes in Mungbean in Jaipur District, Rajasthan, India
Authors: Meena Rahul , Chandrawat B.S. , Manisha  , Midha R.L. 
Published: July 2024

Aim: Mungbean (Vigna radiata (L.) R. Wilczek) is one of the important pulse crops grown in India. Considering the economic importance of nematodes as disease causing organism of mungbean the present investigation was carried out to know the distribution of plant parasitic nematodes in mungbean crops in Jaipur district of Rajasthan.
Methodology: A total 139 soil samples were collected from mungbean fields of 12 different localities in Jaipur district. The samples were processed using Cobb's sieving and decanting technique followed by Baermann's funnel technique and nematode population was counted in suspension and identified on the basis of their morphological characters.
Results: The samples were infected with mainly four genera of plant parasitic nematodes i.e., Meloidogyne spp., Helicotylenchus spp., Pratylenchus spp. and Tylenchorhynchus spp. etc. Out of 139 soil samples, 120 samples showed the presence of Meloidogyne javanica with 86.33 % occurrence, 18.82 % absolute density, 83.81 % relative density and 15.85 % prominence value and found most predominant in different surveyed localities in Jaipur district.
Sodium Dodecyl Sulphate-polyacrylamide Gel Electrophoresis (SDS-PAGE) Characterization of Protein Fractions in Ten Peanut Bunch Type Varieties
Authors: Gore V.B. , Kundan  , Thombre P.R. , Rathod P. J. , Kumar D. , Narale S.B. 
Published: July 2024

Aim: To isolate these fractions, a progressive centrifugation technique was employed, utilizing water, sodium chloride, phosphate buffer, and alcohol.
Place and Duration of Study: A study conducted in department of biochemistry Junagadh Agricultural University, Junagadh for analysis seeds of the bunch-type groundnut were supplied by the Main Oilseeds Research Station in Junagadh during 2020-2022.
The Methodology Used: Using SDS-PAGE separation method, four key protein fractions: albumin, prolamin, globulin, and glutelin were analyzed.
Results: The results revealed the highest albumin and globulin contents ranging from 15.05-20.81% and 69.91-77.99%, respectively. The globulin fraction was found to be significantly higher in proportion compared to the other three fractions. Glutelin and prolamin were found to be very low percentage-wise in bunch varieties but it was found to be in the range of 1.49-3.42 % in glutelin as well as prolamins were found to be in the range of 1.5-3.95%. The SDS-PAGE analysis of the ten-bunch type varieties revealed significant variations in protein profiles, as evidenced by the total number of bands and their respective molecular weight-retention factor (MW-Rf) values. The albumin and globulin fractions exhibited the highest MW-Rf values collectively, whereas glutelin and prolamin demonstrated minimal banding patterns.
Conclusion: This study concludes that the observed qualitative and quantitative differences in seed protein profiles are invaluable for varietal identification.
Sustained Beneficial Effects of a Protocolized Treat‐to‐Target Strategy in Very Early Rheumatoid Arthritis: Three‐Year Results of the Dutch Rheumatoid Arthritis Monitoring Remission Induction Cohort
Authors: Vermeer M. , Kuper H. H. , Moens H. J. Bernelot , Drossaers‐Bakker K. W. , van der Bijl A. E. , van Riel P. L. C. M. , van de Laar M. A. F. J. 
Published: February 2013

ObjectiveTreat‐to‐target (T2T) leads to improved clinical outcomes in early rheumatoid arthritis (RA). The question is whether these results sustain in the long term. Our objective was to investigate the 3‐year results of a protocolized T2T strategy in daily clinical practice.MethodsIn the Dutch Rheumatoid Arthritis Monitoring remission induction cohort, patients newly diagnosed with RA were treated according to a T2T strategy aimed at remission (Disease Activity Score in 28 joints [DAS28] <2.6). Patients were treated with methotrexate, followed by the addition of sulfasalazine, and exchange of sulfasalazine with anti–tumor necrosis factor α agents in case of failure. Primary outcomes were disease activity, Health Assessment Questionnaire (HAQ) score, Short Form 36 physical component summary (PCS) and mental component summary (MCS) scores, and the Sharp/van der Heijde score (SHS) after 3 years. Secondary outcomes were sustained DAS28 remission (≥6 months) and remission according to the provisional American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) definition.ResultsAfter 3 years (n = 342), 61.7% of patients were in DAS28 remission and 25.3% met the provisional ACR/EULAR definition of remission. Sustained remission was experienced by 70.5%, which in the majority was achieved with conventional disease‐modifying antirheumatic drugs only. The median scores were 0.4 (interquartile range [IQR] 0.0–1.0) for the HAQ, 45.0 (IQR 38.4–53.2) for the PCS, 53.1 (IQR 43.2–60.8) for the MCS, and 6.0 (IQR 3.0–13.0) for the total SHS.ConclusionIn very early RA, T2T leads to high (sustained) remission rates, improved physical function and health‐related quality of life, and limited radiographic damage after 3 years in daily clinical practice.
Development of MoS2–CNT Composite Thin Film from Layered MoS2 for Lithium Batteries
Authors: Wang Jia‐Zhao , Lu Lin , Lotya Mustafa , Coleman Jonathan N. , Chou Shu‐Lei , Liu Hua‐Kun , Minett Andrew I. , Chen Jun 
Published: February 2013

AbstractLayered MoS2 prepared by liquid‐phase exfoliation has been blended with single‐walled carbon nanotubes (SWNTs) to form novel composite thin films for lithium battery applications. The films were formed by vacuum filtration of blended dispersions onto nitrocellulose membranes. The resulting composite films were transferred onto Cu foil electrodes via a facile filtration/wet transfer technique from nitrocellulose membranes. The morphology of the film was characterised by field emission scanning electron microscopy, which suggests that the MoS2‐SWNT composite film shows good adherence to the Cu foil substrate. The MoS2‐SWNT composite thin films show strong electrochemical performance at different charge‐discharge rates. The capacity of a MoS2‐SWNT composite film with thickness of 1 μm is approximately 992 mAh g−1 after 100 cycles. The morphology study showed that the MoS2‐SWNT thin film retains structural integrity after 100 cycles, while the MoS2 thin film without SWNTs displays significant cracking. In addition, the novel composite thin film preparation and transfer protocols developed in this study could be extended to the preparation of various layered‐material‐based composite films, with the potential for new device designs for energy applications.
Cutaneous dyspigmentation in patients with ganglioside GM3 synthase deficiency
Authors: Wang Heng , Bright Alicia , Xin Baozhong , Bockoven J.R. , Paller Amy S. 
Published: February 2013

AbstractGanglioside GM3 synthase deficiency is a rare autosomal recessive metabolic disorder characterized by infantile onset of severe irritability and epilepsy, failure to thrive, developmental stagnation, and cortical blindness. Because of the lack of easily recognizable dysmorphism and specific neurologic manifestations, identification of patients with this condition is extremely challenging. Here we report on previously undescribed pigmentary abnormalities in 20 of 38 patients with GM3 synthase deficiency. All 20 of the patients showed freckle‐like hyperpigmented macules, ranging in size from 2 to 5 mm in diameter and usually found bilaterally on the extremities, especially the dorsal aspects of the hands and feet. Seven of these patients also had depigmented macules and patches, especially on the face and extremities. These cutaneous changes were asymptomatic, and were not associated with the severity or particular phenotype of the neurologic disease. They became visible only after the first years of life with an increased incidence with advancing age. These distinct pigmentary features are not identified in 54 normal siblings, and may provide a useful clue in identifying patients with ganglioside metabolic disorders. © 2013 Wiley Periodicals, Inc.
De novo mutations of the gene encoding the histone acetyltransferase KAT6B in two patients with Say‐Barber/Biesecker/Young‐Simpson syndrome
Authors: Szakszon Katalin , Salpietro Carmelo , Kakar Naseebullah , Knegt Alida C. , Oláh Éva , Dallapiccola Bruno , Borck Guntram 
Published: February 2013

AbstractThe Say‐Barber/Biesecker/Young‐Simpson (SBBYS) type of the blepharophimosis–mental retardation syndrome group (Ohdo‐like syndromes) is a multiple congenital malformation syndrome characterized by vertical narrowing and shortening of the palpebral fissures, ptosis, intellectual disability, hypothyroidism, hearing impairment, and dental anomalies. Mutations of the gene encoding the histone‐acetyltransferase KAT6B have been recently identified in individuals affected by SBBYS syndrome. SBBYS syndrome‐causing KAT6B mutations cluster in a ∼1,700 basepair region in the 3′ part of the large exon 18, while mutations located in the 5′ region of the same exon have recently been identified to cause the genitopatellar syndrome (GPS), a clinically distinct although partially overlapping malformation‐intellectual disability syndrome. Here, we present two children with clinical features of SBBYS syndrome and de novo truncating KAT6B mutations, including a boy who was diagnosed at the age of 4 months. Our results confirm the implication of KAT6B mutations in typical SBBYS syndrome and emphasize the importance of genotype–phenotype correlations at the KAT6B locus where mutations truncating the KAT6B protein at the amino‐acid positions ∼1,350–1,920 cause SBBYS syndrome. © 2013 Wiley Periodicals, Inc.
Transgenerational Effects of Variable Foraging Demand Stress in Female Bonnet Macaques
Authors: KINNALLY ERIN L. , FEINBERG CAROLINE , KIM DAVID , FERGUSON KEREL , COPLAN JEREMY D. , MANN J. JOHN 
Published: February 2013

AbstractStress coping is an important part of mammalian life, influencing somatic and mental health, social integration, and reproductive success. The experience of early psychological stress helps shape lifelong stress coping strategies. Recent studies have shown that the effects of early stress may not be restricted to the affected generation, but may also be transmitted to offspring. Understanding whether early stress influences development in subsequent generations may help us understand somewhat why many stress‐related traits and diseases, for which little genetic basis has been discovered, run in families. Experimental early life “variable foraging demand” (VFD) stress has been associated with behavioral hypo‐responsiveness to stress in infant and adolescent bonnet macaques. The present study examined the behavioral effects of experimental early VFD stress in adult bonnet macaques, and further investigated whether non‐exposed adolescent offspring of VFD macaques were also affected. Thirty female bonnet macaques from four rearing histories were observed for behavioral response during stress: adults which had been VFD reared as infants (n = 11), adults which had been Control reared as infants (n = 9), and foraging demand naïve adolescents whose mothers were VFD (n = 4) or Control reared (n = 6). Subjects were observed for behavioral response during two experimental stressor conditions, including: (1) relocation to a novel environment; and (2) relocation with exposure to a “human intruder” making eye contact. Factor analysis yielded five factors that described categories of behavior across stress conditions. While adult VFD and Control reared females unexpectedly did not differ significantly, non‐exposed adolescent offspring of VFD reared mothers displayed significant hypo‐responsiveness in all behavioral categories compared with non‐exposed adolescent offspring of Control females. We suggest that stress hypo‐responsiveness previously observed in adolescent VFD reared animals may abate with age, but is nonetheless observed in the next generation. We conclude that VFD stress affects behavioral development of subsequent generations in non‐human primates. Am. J. Primatol. 75:509‐517, 2013. © 2013 Wiley Periodicals, Inc.
Determination of ferulic acid by flow injection chemiluminescence analysis based on enhancement of the N‐bromobutanimide–eosin–CrCl3 system in alkaline solution
Authors: Shen Guopeng , Jia Xin , Jin Jianwen , Pang Li , Chen Zheng , Du Bin 
Published: February 2013

ABSTRACTA simple and sensitive flow injection chemiluminescence method has been developed for the determination of ferulic acid (FA) based on the significant enhancement effect of FA on the CL signal of the N‐bromobutanimide (NBS)–eosin–CrCl3 system in alkaline solution. Under optimum conditions, the enhanced CL intensity is linearly related to the concentration of FA in its pharmaceutical preparations and human plasma samples. The corresponding linear regression equations were established over the 4.0 × 10–10–1.0 × 10–7 g/mL for FA tablets and 2.0 × 10–10–1.0 × 10–7 g/mL for plasma samples. The limit of detection for FA tablets and limit of quantification for plasma samples were 2.8 × 10–10 g/mL (3 σ) and 3.04 × 10–10 g/mL (10 σ), respectively. A complete analysis could be performed within 40 s, including washing and sampling, giving a throughput of ≈90/h. The proposed method was successfully applied to the determination of FA in pharmaceutical preparations and human plasma samples with satisfactory results. The recoveries of pharmaceutical preparations and human plasma samples at three different concentrations were 97.8–102.6% and 96.7–104.0%, respectively. Furthermore, the possible mechanism of CL reactions was also discussed briefly. Copyright © 2013 John Wiley & Sons, Ltd.
Simultaneous determination of eleven major components in Fugan Fang using high‐performance liquid chromatography coupled with mass spectrometry
Authors: Jiang Peng , Shi Rong , Wang Qian , Ma Yue‐Ming , Cui Hong‐Yan , Liu Ping , Liu Cheng‐Hai 
Published: February 2013

ABSTRACTFugan fang (FGF) is a traditional Chinese medicine (TCM) prescription that has been used in treating hepatic illnesses for many years. In this study, an analytical method is developed for the quantitative analysis of the major components of FGF. This method is based on high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS) on a reverse‐phase C18 column. Results show that 0.01% acetic acid and acetonitrile is the optimum mobile phase in gradient elution. All compounds showed good linearity (r2 ≥ 0.9948). Recoveries measured at three concentration levels varied from 83.5 to 104.8%. The method was validated with respect to precision, repeatability and accuracy and was successfully applied in the quantification of the 11 components of FGF products. The validated HPLC‐MS method provides a basis for assessing the quality of TCM prescriptions containing many bioactive components. Copyright © 2013 John Wiley & Sons, Ltd.
Environmental Innovations as a Source of Competitive Advantage or Vice Versa?
Authors: Forsman Helena 
Published: February 2013

ABSTRACTThe aim of this paper is to examine the links between developed environmental innovations and the competitiveness of firms. It seeks answers to the question: Are the developed environmental innovations associated with the improved or impaired competitiveness of firms? In addition, it explores how competitive advantage is created along the innovation process. This will be done by comparing the successful and unsuccessful green innovators. The empirical evidence is based on the longitudinal dataset gathered from 128 Finnish firms which have developed one or more environmental innovations. The data covers nine years from 2002 to 2010. This study provides two contributions to academic literature. First, it deepens the existing knowledge of how environmental innovations are associated with competitive advantage. It identifies the types of competitive advantages as well as potential disadvantages along the innovation process. Second, this study demonstrates how the competitive advantage was enhanced along the successful innovation process. Copyright © 2013 John Wiley & Sons, Ltd and ERP Environment
Evaluation of 5‐year imatinib treatment of 458 patients with CP‐CML in routine clinical practice and prognostic impact of different BCR‐ABL cutoff levels
Authors: Klamová Hana , Poláková Kateřina Machová , Mužík Jan , Ráčil Zdeněk , Žáčková Daniela , Steinerová Kateřina , Karas Michal , Faber Edgar , Demečková Eva , Michalovičová‐Sninská Zuzana , Voglová Jaroslava , Demitrovičová Ľudmila , Mikušková Eva , Tóthová Elena , Chudej Juraj , Markuljak Imrich , Cmunt Eduard , Moravcová Jana , Dvořáková Dana , Michalová Kyra , Jarošová Marie , Šťastná Markéta Marková , Cetkovský Petr , DuŠek Ladislav , Koza Vladimír , Trněný Marek , Indrák Karel 
Published: February 2013

AbstractWe evaluated responses to the treatment and long‐term outcomes of chronic myeloid leukemia patients treated with imatinib as first‐line treatment in routine clinical setting from two countries with centralized tyrosine kinase inhibitors (TKIs) treatment. We assessed prognostic significance of European LeukemiaNet (ELN) 2006‐ and 2009‐defined responses and the prognostic value of molecular responses at defined time points on 5‐year survivals. Among the cumulative rates of incidence of hematologic, cytogenetic, and molecular responses and all important survival parameters, we evaluated the prognostic significance of different BCR‐ABL transcript‐level ratios (≤1%; >1%–≤10%; >10%) at 3, 6, 12, and 18 months (n = 199). The ELN optimal response criteria and their predictive role were significantly beneficial for event‐free survival at all given time points. We found significant improvement in survivals of patients with BCR‐ABL lower than 10% in the 6th and 12th months. Significantly better outcome was found in patients who achieved major molecular response (MMR) in the 12th month. The cumulative incidences of complete cytogenetic response (CCyR) and MMR were significantly associated with the molecular response in the 3rd month. The ELN response criteria and their predictive role were helpful at given time points; however, the 2009 definition did not significantly alter the prognostic accuracy compared with that of the 2006 definition. The significant value was observed for cytogenetic responses at the 6th and 12th month. Moreover, progression‐free and event‐free survivals were improved with MMR at the 12th month.
Quest for the Ideal Cancer Biomarker: An Update on Progress in Capture and Characterization of Circulating Tumor Cells
Authors: Harris Jennifer L. , Stocum Michael , Roberts Lisa , Jiang Chunsheng , Lin Jessie , Sprott Kam 
Published: February 2013

Abstract

Preclinical Research

The quest for a well‐validated, non‐invasive biomarker to aid in clinical decision making has remained elusive in the cancer space over the last 30 years. Much promise has been attached to circulating tumor cells (CTCs) as prognostic, predictive, and pharmacodynamic biomarkers with the potential to eliminate the need for invasive tumor biopsies and improve on the clinical value of other circulating tumor markers. The CellSearch® system (Veridex, LLC., Raritan, NJ, USA) cleared the U.S. Food and Drug Administration when the presence of CTCs was shown to have prognostic significance in patients with breast, prostate, and colorectal cancer. However, CTCs are not, at present, routinely being used in the clinic to guide treatment decisions. This paper discusses key attributes that a biomarker must possess, the status of other potential cancer biomarkers, and advancements in the capture and characterization of CTCs that will enable actualization of their potential as a reliable and efficient biomarker of disease diagnosis, progression, and response to therapy in the clinic.
Lack of Mycobacterium tuberculosis–specific interleukin‐17A–producing CD4+ T cells in active disease
Authors: Perreau Matthieu , Rozot Virginie , Welles Hugh C. , Belluti‐Enders Felicitas , Vigano Selena , Maillard Michel , Dorta Gian , Mazza‐Stalder Jesica , Bart Pierre‐Alexandre , Roger Thierry , Calandra Thierry , Nicod Laurent , Harari Alexandre 
Published: February 2013

Protective immunity to Mycobacterium tuberculosis (Mtb) is commonly ascribed to a Th1 profile; however, the involvement of Th17 cells remains to be clarified. Here, we characterized Mtb‐specific CD4+ T cells in blood and bronchoalveolar lavages (BALs) from untreated subjects with either active tuberculosis disease (TB) or latent Mtb infection (LTBI), considered as prototypic models of uncontrolled or controlled infection, respectively. The production of IL‐17A, IFN‐γ, TNF‐α, and IL‐2 by Mtb‐specific CD4+ T cells was assessed both directly ex vivo and following in vitro antigen‐specific T‐cell expansion. Unlike for extracellular bacteria, Mtb‐specific CD4+ T‐cell responses lacked immediate ex vivo IL‐17A effector function in both LTBI and TB individuals. Furthermore, Mtb‐specific Th17 cells were absent in BALs, while extracellular bacteria‐specific Th17 cells were identified in gut biopsies of healthy individuals. Interestingly, only Mtb‐specific CD4+ T cells from 50% of LTBI but not from TB subjects acquired the ability to produce IL‐17A following Mtb‐specific T‐cell expansion. Finally, IL‐17A acquisition by Mtb‐specific CD4+ T cells correlated with the coexpression of CXCR3 and CCR6, currently associated to Th1 or Th17 profiles, respectively. Our data demonstrate that Mtb‐specific Th17 cells are selectively undetectable in peripheral blood and BALs from TB patients.
The Linkage Between the Options and Credit Default Swap Markets During the Subprime Mortgage Crisis
Authors: Kim Tong Suk , Park Yuen Jung , Noh Jaesun 
Published: February 2013

AbstractThis study investigates the linkage between the options and credit default swap (CDS) markets around the subprime mortgage crisis period, using the unit recovery claim (URC). Through estimation of a well‐hedging strike price within a firm‐specific and time‐varying default corridor and consideration of the CDS term structure and firm‐specific recovery rate, modified URCs from the two markets are demonstrated to have a tighter linkage. The adjusted URCs show that, after the crisis, the effect of macroeconomic variable on deviations between the two markets increased and the options market's predictive power for the future movement of the CDS market was amplified. © 2013 Wiley Periodicals, Inc. Jrl Fut Mark
Drug‐Induced Liver Injury: The Role of Drug Metabolism and Transport
Authors: Corsini Alberto , Bortolini Michele 
Published: February 2013

AbstractMany studies have pinpointed the significant contribution of liver‐mediated drug metabolism and transport to the complexity of drug‐induced liver injury (DILI). Phase I cytochrome P450 (CYP450) enzymes can lead to altered drug metabolism and formation of toxic metabolites, whilst Phase II enzymes are also associated with DILI. The emerging role of hepatic transporters in regulating the movement of endogenous and exogenous chemicals (e.g., bile acids and drugs) across cellular and tissue membranes is critical in determining the pathophysiology of liver disease as well as drug toxicity and efficacy. Genetic and environmental factors can have a significant impact on drug metabolism and transporter proteins, consequently increasing the risk of DILI in susceptible individuals. The assessment of these factors therefore represents an important approach for predicting and preventing DILI, by better understanding the pharmacological profile of a specific drug. This review focuses on the mechanisms of DILI associated with drug metabolism and hepatic transport, and how they can be influenced by underlying factors.