bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilization of mental health services and associated factors among residents of southern Ethiopia; a community based cross-sectional study
Authors: Alamirew Birhanu , Darge Beniam D. , Terefe Bezabih , Gebremeskel Feleke 
Published: February 2025

Abstract

Background
In 2019, nearly one billion individuals worldwide were estimated to be living with some form of mental illness. This staggering figure underscores not only the widespread prevalence of mental health issues but also their significant negative impact. Despite the critical impact of mental health problems, there is a substantial gap in treatment at the global scale. Particularly in rural Ethiopia, there is a notable lack of data regarding the use of mental health services by community residents. This study was conducted with the aim of evaluating the utilization of mental health services and identifying factors that influence access to these services among the population of rural southern Ethiopia.

Methods
A community-based cross-sectional study was conducted on randomly selected adults in southern Ethiopia. A semi-structured questionnaire assessing the sociodemographic status of the participants was used. Depression and anxiety were measured with the Patient Health Questionnaire (PHQ-9) and the General Anxiety Disorders Scale (GAD-7), respectively. The data were then analyzed using StataMP version 18. Statistical tests such as frequency, percentage, mean, bivariate logistic regression, and multivariate analysis were performed. P values and confidence intervals were used to determine statistical significance.

Results
A total of 971 participants were enrolled in this study, and the mean age was 34.9 (± 11) years. A minority of the participants (152 [15.5%]) sought some form of help for mental health-related reasons. Only 24 (2.5%) of the participants used biomedical mental health services. The perceived need for any mental health service was 96 (9.9%). Thirty-three percent of the respondents with mild to moderate depression and 37.1% of those with severe depression sought care for their concerns. Similarly, 37% and 35% of individuals with mild to moderate anxiety and severe anxiety, respectively, utilized mental health services. A higher monthly income, psychoactive substance use, perceived need, and symptoms of depression and anxiety were significantly associated with mental health service utilization.

Conclusion
Our study revealed a significantly low level of utilization of mental health services among the residents of rural southern Ethiopia. More efforts to address the treatment gap for mental health problems in the country are needed.
Emergence of population heterogeneity in Klebsiella pneumoniae with a blaOXA-232-harboring plasmid: carbapenem resistance, virulence, and fitness
Authors: Cho Yun Young , Kim Sun Ju , Ko Kwan Soo 
Published: February 2025

Abstract

Background
This study aimed to investigate the population heterogeneity on carbapenem susceptibility in Klebsiella pneumoniae strains that acquired a bla
OXA-232-bearing ColE-type plasmid.

Methods
A bla
OXA-232-bearing plasmid was electroporated into two carbapenem-susceptible K. pneumoniae strains. High- and low-carbapenem-resistant subpopulations were identified and isolated using patch plating. The strains were subsequently subcultured in antibiotic-free media, yielding two distinct populations: a stable, high-level carbapenem-resistant strains and a heterogeneous strains. Antibiotic susceptibility tests, time-killing assays, and population profiles were conducted, along with a competition assay was performed and the growth curve analysis. To assess virulence, we performed human serum resistance and Galleria mellonella infection assays, and measured the expression of virulence genes using qRT-PCR. Additionally, whole genome sequencing was carried out for further anaysis.

Results
Introduction of pOXA-232 into carbapenem-susceptible K. pneumoniae strains resulted in two isogenic transformants with distinct resistance profiles: an unstable, high-level carbapenem-resistant (HCR), and highly virulent subpopulation; and a stable, low-level carbapenem-resistant (LCR), and low-virulence subpopulation. Whole genome and expression analyses revealed dysfunctionality of ompK36 in HCR subpopulations. Subculturing of HCR led to the re-emergence of heterogeneous populations with variations in carbapenem resistance and an additional compensatory mutation of 9,000 bp deletion in the genome. Thus, stable HCR strains featuring both mutations in ompK36 and compensatory mutations developed.

Conclusion
This study demonstrated that underlying heterogeneity can promote the emergence of stable, high-level antibiotic resistance, even with the introduction of a plasmid carrying a low-level antibiotic resistance gene, such as bla
OXA-232. This highlights the critical need to closely monitor bacterial population dynamics.
Lower limb pointing to assess intersegmental dynamics after incomplete spinal cord injury and the associated role of proprioceptive impairments
Authors: Malik Raza N. , Marigold Daniel S. , Chow Mason , Eginyan Gevorg , Lam Tania 
Published: February 2025

Abstract

Background
Disorders in the recovery of gait strategies in individuals with incomplete spinal cord injury (SCI) suggest difficulties in controlling lower limb intersegmental dynamics, which could relate to proprioceptive impairments. To probe discrete aspects of lower limb interjoint coordination, we present here a novel protocol to assess lower limb motor strategies and evaluate the influence of proprioceptive impairments following SCI.

Methods
Twelve able-bodied controls and 16 participants with SCI performed lower limb pointing to three targets that involved combined hip and knee flexion, or hip or knee flexion only while standing, with either full or obstructed visual feedback. We quantified lower limb proprioceptive sense in individuals with SCI using a robotic gait device. We used motion analysis to determine lower limb joint angles and foot trajectory, computed inverse dynamics to quantify joint and intersegmental dynamics, and derived muscle torque as an indicator of the motor strategies produced to control the motion to each target. We used linear mixed-effects models to assess differences between the control and SCI groups on end-point performance and muscle torque, and to assess the relationship of muscle torque with end-point performance and proprioceptive sense.

Results
Groups differed in motor strategies, but not end-point performance, when pointing to all three targets. Compared to controls, the SCI group had difficulty controlling knee muscle torque when performing the hip-flexion-only target (p = 0.008) or when flexing the hip and knee simultaneously (p = 0.0004). To complete the knee-flexion-only target, the SCI group had difficulties generating the required hip extensor muscle torque to maintain the thigh in neutral (p = 0.0001). These altered motor strategies in individuals with SCI were associated with proprioceptive impairments and end-point performance.

Conclusion
This novel lower limb pointing task can identify disordered motor strategies in individuals with SCI, especially at the knee, and are associated with proprioceptive impairment. Variations of this paradigm can be employed to further understand differences in motor strategies between controls and individuals with SCI, and the impact of proprioceptive deficits.
Engineered oncolytic virus coated with anti-PD-1 and alendronate for ameliorating intratumoral T cell hypofunction
Authors: Zhu Yufu , Zhang Xuefeng , Jin Jiaqi , Wang Xiaoqian , Liu Yang , Gao Jian , Hang Diancheng , Fang Lin , Zhang Hengzhu , Liu Hongmei 
Published: February 2025

Abstract

Background
Glioblastoma is a highly aggressive and devastating primary brain tumor that is resistant to conventional therapies. Oncolytic viruses represent a promising therapeutic approach for glioblastoma by selectively lysing tumor cells and eliciting an anti-tumor immune response. However, the clinical efficacy of oncolytic viruses is often hindered by challenges such as short persistence, host antiviral immune responses, and T cell dysfunction.

Methods
We have developed a novel therapeutic strategy by “dressing” oncolytic viruses with anti-PD-1 antibodies and alendronate (PD-1/Al@OV) to prevent premature clearance of the oncolytic viruses and enhance T cell function, thereby improving immunotherapy outcomes against glioma.

Results
We found that in the high reactive oxygen species environment of the tumor, PD-1/Al@OV disassembled to release oncolytic viruses, anti-PD-1, and alendronate. The released anti-PD-1 blocked the PD-1/PD-L1 pathway, activating T cells; the alendronate eliminated tumor-associated macrophages, increasing the concentration of oncolytic viruses; and the oncolytic viruses directly lysed cancer cells, enhancing intratumoral T cell infiltration.

Conclusion
This approach effectively improved the immunosuppressive microenvironment of glioblastoma and achieved a robust anti-tumor effect. Consequently, this study presents a novel strategy for immune combination therapy and the improvement of the glioblastoma immune microenvironment, thereby offering new prospects for the clinical application of oncolytic viruses.
Association among parents’ stress recovery experiences, parenting practices, and children’s behavioral problems: a cross-sectional study
Authors: Hosokawa Rikuya , Katura Toshiki 
Published: February 2025

Abstract

Background
Parents can experience much stress from parenting, work, and household responsibilities. Parents’ stress recovery experiences, or their lack thereof, can affect parenting practices and influence children’s behavioral problems, which may thereby lead to difficulties for children later in life. Therefore, the relationships among these three factors deserve consideration. This study tested a model of the mediating role of parenting practices in the relationship between parents’ stress recovery experiences and children’s behavioral problems.

Methods
Parents (N = 1,112) of 14-year-old children in the third year of junior high school in Japan completed a questionnaire, yielding 583 valid responses. To accurately determine the relationship among parents’ stress recovery experiences, parenting practices, and children’s behavioral problems, parents of children diagnosed with developmental disabilities and parents who did not respond to the required items in the questionnaire were excluded from the analysis. As a result, 536 of the 583 (89.0%) parents met the inclusion criteria. We conducted a path analysis, following the hypothesis that parents’ stress recovery experiences, via their parenting practices, are associated with children’s behavioral problems.

Results
The path analysis results indicated that parents’ stress recovery experiences of relaxation and mastery were positively associated with positive nurturing attitudes, whereas mastery and control were negatively associated with negative nurturing attitudes. Furthermore, positive nurturing attitudes were negatively associated with externalizing and internalizing problem behaviors, whereas negative nurturing attitudes were positively associated with externalizing and internalizing problem behaviors. In other words, the hypothesis that parents’ stress recovery experiences of relaxation, mastery, and control reduce children’s behavioral problems via promoting nurturing parental attitudes was supported.

Conclusions
The results indicate that the higher the level of parents’ stress recovery experiences, the lower the level of reported children’s behavioral problems. Parents’ stress recovery experiences correlated with parenting practices, which partially mediated the relationship of the parents’ stress recovery with children’s behavioral problems. The suggestion is that increasing parents’ stress recovery experiences, improving parenting practices and related behaviors, and strengthening the parent–child relationship are important measures that can be mutually beneficial for parents, children, and the overall family relationship.
Retinal ganglion cell vulnerability to pathogenic tau in Alzheimer’s disease
Authors: Davis Miyah R. , Robinson Edward , Koronyo Yosef , Salobrar-Garcia Elena , Rentsendorj Altan , Gaire Bhakta P. , Mirzaei Nazanin , Kayed Rakez , Sadun Alfredo A. , Ljubimov Alexander V. , Schneider Lon S. , Hawes Debra , Black Keith L. , Fuchs Dieu-Trang , Koronyo-Hamaoui Maya 
Published: February 2025

Abstract
Pathological tau isoforms, including hyperphosphorylated tau at serine 396 (pS396-tau) and tau oligomers (Oligo-tau), are elevated in the retinas of patients with mild cognitive impairment (MCI) due to Alzheimer’s disease (AD) and AD dementia. These patients exhibit significant retinal ganglion cell (RGC) loss, however the presence of tau isoforms in RGCs and their impact on RGC integrity, particularly in early AD, have not been studied. Here, we analyzed retinal superior temporal cross-sections from 25 MCI or AD patients and 16 age- and sex-matched cognitively normal controls. Using the RGC marker ribonucleic acid binding protein with multiple splicing (RBPMS) and Nissl staining, we found a 46–56% reduction in RBPMS+ RGCs and Nissl+ neurons in the ganglion cell layer (GCL) of MCI and AD retinas (P < 0.05–0.001). RGC loss was accompanied by soma hypertrophy (10–50% enlargement, P < 0.05–0.0001), nuclear displacement, apoptosis (30–50% increase, P < 0.05–0.01), and prominent expression of granulovacuolar degeneration (GVD) bodies and GVD-necroptotic markers. Both pS396-tau and Oligo-tau were identified in RGCs, including in hypertrophic cells. PS396-tau+ and Oligo-tau+ RGC counts were significantly increased by 2.1–3.5-fold in MCI and AD retinas versus control retinas (P < 0.05–0.0001). Tauopathy-laden RGCs strongly inter-correlated (r

P
=0.85, P < 0.0001) and retinal tauopathy associated with RGC reduction (r

P
=-0.40–(-0.64), P < 0.05–0.01). Their abundance correlated with brain pathology and cognitive deficits, with higher tauopathy-laden RGCs in patients with Braak stages (V–VI), clinical dementia ratings (CDR = 3), and mini-mental state examination (MMSE ≤   26) scores. PS396-tau+ RGCs in the central and mid-periphery showed the closest associations with disease status, while Oligo-tau+ RGCs in the mid-periphery exhibited the strongest correlations with brain pathology (NFTs, Braak stages, ABC scores; r

S
=0.78–0.81, P < 0.001–0.0001) and cognitive decline (MMSE; r

S
=-0.79, P = 0.0019). Overall, these findings identify a link between pathogenic tau in RGCs and RGC degeneration in AD, involving apoptotic and GVD-necroptotic cell death pathways. Future research should validate these results in larger and more diverse cohorts and develop RGC tauopathy as a potential noninvasive biomarker for early detection and monitoring of AD progression.
Spatiotemporal dynamics reveal high turnover and contrasting assembly processes in fungal communities across contiguous habitats of tropical forests
Authors: Lin Chieh-Ping , Lin Yu-Fei , Liu Yu-Ching , Lu Mei-Yeh Jade , Ke Huei-Mien , Tsai Isheng Jason 
Published: February 2025

Abstract

Background
The variation in fungal community composition within a single habitat space has been extensively studied in forest ecosystems. However, the spatial and temporal distribution of fungi across contiguous habitats, particularly at a local scale and in tropical regions, remains underexplored. In this study, we examined the fungal community composition across multiple habitats proximal to each other over two seasons in seven Fagaceae species in Taiwanese broadleaf forests. We tested how local spatial scale and habitat influence community assembly.

Results
Using a metabarcoding approach, we sequenced ITS2 regions from 864 samples collected from four distinct habitats—leaves, twigs, litter, and soil. We identified 11,600 fungal amplicon sequence variants (ASVs), with community composition differing significantly between habitats proximal to each other. Generalized dissimilarity modeling (GDM) revealed that spatial distance, interacting with precipitation, was the strongest predictor of fungal turnover, particularly in the phyllosphere. Normalized Stochasticity Ratio (NST) analyses further highlighted contrasting assembly processes, with deterministic influences dominating in the phyllosphere habitat, while stochasticity prevailed in soil and litter. Random forest analysis accurately classified habitats based on ASVs’ relative abundances, with strong predictors were mostly habitat-specific ASVs prevalent in soil. Misclassified samples were due to secondary contact of fungi between adjacent habitats. Co-occurrence network analysis revealed more complex and deterministic networks in leaf and twig habitats, while soil was driven by stochastic processes and contained most habitat-specific ASVs. A Cladosporium sp. emerged as a keystone species, maintaining network stability across forests.

Conclusion
This study reveals how local spatial variation and habitat shape distinct fungal communities in tropical forests, with deterministic processes dominating in some habitats and stochasticity playing a key role in others. We show extremely high turnover in fungal community are present over very short distances and that local fungal taxa are strong habitat predictors. These findings highlight the importance of studying coexisting habitats to gain a deeper understanding of fungal biogeography and ecosystem function.
Current status of forensic anthropology in the Middle East and North Africa (MENA) region
Authors: Mansour Cindy , Márquez-Grant Nicholas , Benito Sánchez María 
Published: February 2025

Abstract

Background
Forensic anthropology has evolved significantly, from its foundations in the nineteenth century to its formal establishment in the twentieth century and in particular with modern advancements from the 1970s onward. Its role in human rights investigations during the 1980s in Latin America and the 1990s in the Balkans, exemplifies its global impact. However, the practice and application of forensic anthropology in the Middle East and North Africa (MENA) region remain underexplored. This study assesses the current status of forensic anthropology in this region through a brief literature review and online interviews with academics and practitioners in forensic anthropology or closely related disciplines. The interviews addressed the medico-legal system structure, forensic science capabilities, training efforts, practitioner availability, case types (medico-legal and humanitarian), and resources like radiological imaging.

Results
The study revealed that forensic anthropology is largely underutilized in the MENA region’s medico-legal death investigation systems. Factors such as limited human capital, lack of discipline awareness, varying legal and procedural systems, and insufficient academic infrastructure hinder its integration. Challenges include political instability, safety concerns for practitioners, and inadequate resources. The study highlights ongoing efforts by practitioners to improve the field through theoretical and practical training, capacity building, and resource development.

Conclusion
The findings underscore the need for strategic investments to strengthen forensic anthropology in the MENA region. Recommendations include enhancing education and training, fostering interdisciplinary collaboration, disseminating scientific knowledge, increasing access to resources, and revising medico-legal frameworks. These measures can bridge existing gaps and advance forensic anthropology’s role in medico-legal and humanitarian contexts.
Vagus nerve stimulation combined with nerve rehabilitation therapy for upper limb paralysis after hemorrhagic stroke: a stroke-related epilepsy case
Authors: Xia Chunsheng , Xu Peng , Wang Lanlan , Zhang Dong , Qi Yinbao , Wu Ming , Qian Ruobing 
Published: February 2025

Abstract

Background
Hemorrhagic stroke has a high incidence, often leaving patients with significant complications such as limb mobility disorders after treatment. Traditional treatment methods for stroke patients mainly include limb function exercises and hyperbaric oxygen therapy, which have shown effective results. In recent years, there have been reports utilizing vagus nerve stimulation (VNS) to treat limb paralysis in ischemic stroke patients, achieving encouraging outcomes. However, there are rare related reports on hemorrhagic stroke.

Case presentation
This report presents a case of a patient who developed left upper limb hemiplegia and recurrent seizures after a hemorrhagic stroke. The patient showed a poor response to standard anti-epileptic treatment and was diagnosed with stroke-related epilepsy. To manage the recurrent seizures, VNS was performed. After the device was activated, the patient reported a significant reduction in abnormal muscle tone and increased mobility impairment in the affected upper limb. Parameters were adjusted, and intermittent stroke electrical stimulation was combined with upper limb rehabilitation exercises. After three months of active treatment, the patient’s seizures were well controlled, and there was significant improvement in upper limb function.

Conclusions
VNS has potential in the rehabilitative treatment of stroke patients with upper limb dysfunction. It is hoped that more patients will benefit from this advanced treatment method in the future, regaining their health and vitality. Additionally, future research needs to further explore the mechanisms and methods of brain remodeling to provide theoretical support and more effective treatment options for stroke patient rehabilitation.
Stability of Anti-Drug Antibodies Against Antibody Therapeutics
Authors: Sirohi Parul , Hainline Kelly , Bullock Heather , Wang Sihe , Konrad Robert J. , Wen Yi 
Published: February 2025

Abstract
Biotherapeutics have the potential to elicit unwanted immune responses that can lead to the production of anti-drug antibodies (ADA). It is critical that ADA responses are detected, characterized, and monitored to understand the safety and efficacy of a drug. ADA samples must remain stable in long- and short-term storage conditions to ensure reliable analysis. Whereas the stability of anti-vaccine antibodies has been well-studied, there are few reports examining the stability of anti-therapeutic antibodies using clinical samples. In this study, ADA samples from four clinical trials of antibody therapeutics were found to be stable after long-term storage (1–10 years) at -80°C and short-term storage (24 h to two weeks) at 4°C, 22°C, and 37°C. In addition, samples were stable after 16 freeze/thaw cycles. The results demonstrate the stability of ADA in clinical samples under various conditions. Consequently, the results observed herein suggest that the routine assessment of ADA sample stability may not be warranted.

Graphical Abstract