bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis of Pregna-1,4,16-triene-3,20-dione from a Mixture of Soybean Phytosterols using a Combination of Chemical and Microbiological Methods
Authors: Huy Luu Duc , Savinova Tatiana Stepanovna , Kazantsev Alexey Vitalievich , Fokina Victoria Valerievna , Donova Marina Viktorovna 
Published: August 2024

:
In this paper, we present an efficient procedure for the transformation of a soybean phytosterol mixture
into pregna-1,4,16-triene-3,20-dione, which is a key intermediate for the synthesis of valuable corticoids.
The possibility of using two alternative methods for 1(2)-dehydrogenation of pregna-4,16-diene-3,20-dione -
chemical and microbiological - was shown. Microbiological 3-keto-4-ene steroid 1(2)-dehydrogenation was
carried out using actinobacterial cells of Nocardioides simplex VKM Ac-2033D. The structures of the synthesized
compounds were confirmed by the IR, MS, and 1H-NMR methods.
Whole Genome Sequences of Cryptotympana Atrata Fabricius, 1775 (Hemiptera: Cicadidae) in the Korean Peninsula: Insights into Population Structure with Novel Pathogenic Or Symbiotic Candidates
Authors: Park Jeong Sun , Kim Jina , Kim Yeha , Kim Ki Hwan , Kwak Woori , Kim Iksoo 
Published: August 2024

Background:
The blackish cicada (Cryptotympana atrata) exhibits unique characteristics
and is one of the model cicadas found in the Korean Peninsula. It is a species of southern origin,
prefers high temperatures, and is listed as a climate-sensitive indicator species in South Korea.
Therefore, this species can be utilized to study the impact of climate change on the genetic diversity
and structure of populations. However, research on the genome of C. atrata is limited.

Methods:
We sequenced the genome of an individual collected from South Korea and constructed
a draft genome. Additionally, we collected ten specimens from each of the five regions in South
Korea and identified single nucleotide variants (SNVs) for population genetic analysis. The sequencing
library was constructed using the MGIEasy DNA Library Prep Kit and sequenced using
the MGISEQ-2000 platform with 150-bp paired-end reads.

Results:
The draft genome of C. atrata was approximately 5.0 Gb or 5.2 Gb, making it one of the
largest genomes among insects. Population genetic analysis, which was conducted on four populations
in South Korea, including both previously distributed and newly expanded regions, showed
that Jeju Island, a remote southern island with the highest average temperature, formed an independent
genetic group. However, there were no notable genetic differences among the inland populations
selected based on varying average temperatures, indicating that the current population genetic
composition on the Korean Peninsula is more reflective of biogeographic history rather than climate-
induced genetic structures. Additionally, we unexpectedly observed that most individuals of
C. atrata collected in a specific locality were infected with microbes not commonly found in insects,
necessitating further research on the pathogens within C. atrata.

Conclusion:
This study introduces the draft genome of C. atrata, a climate-sensitive indicator species
in South Korea. Population analysis results indicate that the current genetic structure of C.
atrata is driven by biogeographic history rather than just climate. The prevalence of widespread
pathogen infections raises concerns about their impact on C. atrata. Considering the scarcity of
publicly available genomic resources related to the family Cicadidae, this draft genome and population
data of C. atrata are expected to serve as a valuable resource for various studies utilizing cicada
genomes.
Targeting the Tumor Epigenetic Regulator SETDB1 for Tumor Therapy
Authors: Peng Cheng , Zhou Nini , Chen Tengjiang , Lei Jie , Chen Changwen , Zhu Shunqin 
Published: August 2024

Abstract:
Epigenetic alterations are implicated in the early stages of tumorigenesis and are widely recognized as a ubiquitous phenomenon in cancer development. Aberrant epigenetic modifications can alter the expression of target genes, induce heterochromatin formation, and gradually drive normal cells towards immortalized tumor cells with significant consequences. SETDB1 (SET domain bifurcated histone lysine methyltransferase 1), a typical histone me-thyltransferase, promotes the formation of heterochromatin and inhibits the transcription of genes by modifying the methylation of lysine 9 of histone 3. SETDB1 is usually highly ex-pressed in tumors with high copy numbers, accompanied by poor prognosis and low patient survival rates, which is a typical case of abnormal epigenetic modification. We discuss the mechanism of SETDB1 in a variety of cancers and review the epigenetic inhibitors that have been reported in recent years, along with their anti-tumor effects. In addition, we summarize the role of SETDB1 in a variety of diseases and cell functions.
Advancements in Combating Fungal Infections: A Comprehensive Review on the Development of Azole Hybrid Antifungals
Authors: Ankita  , Thakur Shorya , Verma Rupali , Kumar Rajesh , Khurana Navneet , Kaur Charanjit , Singh Gurvinder 
Published: August 2024

Objective:
In this review, we have summarized antifungal agents containing potent
azole analogues.

Data acquisition:
The provided literature is related to the development and application of azole
derivatives and has been accessed from electronic data bases such as Science direct, Google
Scholar, and Pubmed using keywords such as “design, synthesis and evaluation”, “azole hybrids”,
“diazole hybrids”, “indazole derivatives”, “imidazole derivatives”, “triazole derivatives”,
“tetrazole derivatives” and related combinations.

Result:
From this review, it was identified that azole derivatives with promising antifungal activity
play a vital role in drug discovery and development. The literature revealed that azole derivatives
can effectively fight several types of microorganisms, such as Candida albicans, Aspergillus
niger, and others. The rational design and structure‒activity relationship of these compounds
are discussed in this paper, highlighting their potential as effective therapeutic options
against various fungal pathogens. Moreover, this work addresses the challenges and future directions
in the development of azole hybrids. The results of docking studies of several of the hybrids
that the researchers provided are also summarized.

Conclusion:
The current work attempts to review such innovations, which may lead to the preparation
of novel therapeutics. More research is required to confirm their safety and effectiveness
in clinical practices.
The m6A Reader IGF2BP2 Promotes Oral Squamous Cell Carcinoma Progression by Maintaining UCA1 Stability
Authors: Zhang Duheyi , Mai Lianxi , Zhang Lizao , Huang Guoxin , Lin Zhaoyu , Wang Shuang , Rao Guangxin , Xie Shule , Pan Chaobin 
Published: August 2024

Introduction:
N6-methyladenosine (m6A) modifications of RNAs are associated with
many cancer types. Nevertheless, the function of the m6A reader IGF2BP2 in oral squamous cell
carcinoma (OSCC) has yet to be ascertained.

Aims:
The objective of this investigation was to elucidate the role of IGF2BP2 in OSCC and delineate
the associated mechanisms.

Method:
Elevated expression of IGF2BP2 was observed in OSCC, and this overexpression significantly
correlated with adverse prognostic outcomes in patients with OSCC. In vitro analyses demonstrated
that silencing of IGF2BP2 attenuated the proliferation, migration, and invasion capabilities
of oral cancer cells while concurrently promoting apoptosis.

Results:
In vivo experiments demonstrated that IGF2BP2 promoted OSCC growth. RNA-seq and
m6A-seq were utilized to elucidate the downstream targets of IGF2BP2. Through bioinformatic
analysis, we identified the long noncoding RNA (lncRNA) UCA1 as a target. IGF2BP2 was found
to maintain the stability of UCA1 in an m6A-dependent manner by binding to m6A-modified
UCA1 and plays an oncogenic role in OSCC through UCA1.

Conclusion:
In conclusion, we identified IGF2BP2 as a prognostic biomarker of OSCC, and the
IGF2BP2-UCA1 axis was found to promote OSCC progression and may perform as a novel therapeutic
target.
Exploring the Mitigation Actions of the Epidemic Impacts on Construction Projects in the Kingdom of Saudi Arabia
Authors: Abdullatif Abdullah M. , Abdulghafour Abdulrazak B. , Mlybari Ehab A. , Alsulami Badr T. 
Published: August 2024

Background
The construction industry has an important role in the economy of countries, as the construction industry represents 6.1 percent of the gross domestic product in the Kingdom of Saudi Arabia. A global disaster such as an epidemic causes long-term damage to society, the economy, and related industries. The recent COVID-19 epidemic is one of such disasters. The construction industry was greatly affected by the epidemic globally, as well as in the Kingdom of Saudi Arabia.

Methods
This research aims to determine the impact of the epidemic on construction projects and shed light on the actions taken to mitigate the impact of the epidemic through a multiple case study of three construction projects in the Kingdom of Saudi Arabia, where a questionnaire and a semi-structured interview were conducted with professionals in these projects. Descriptive analysis and the Relative Importance Index (RII) were used.

Results
The impact was of high importance on the project time throughout all projects, especially on the project schedule; the RII value was the maximum. Secondly for material delivery within time ranged from 0.8 – 0.91 for RII value and in terms of the mitigation actions taken, communication between project management and employees witnessed maximum effectiveness reached to 83% of participants reported a very effective response, with the help of modern technologies. Most factors ranged from limited effectiveness to ineffective.

Conclusion
This research contributes to determine the effects of the epidemic and the actions taken to mitigate it, which benefits the organizations and stakeholders in the field of construction and engineering project management, to develop and improve actions, and to prepare and plan for similar circumstances in the future.
KOMPARASI UPAH BORONGAN DAN UPAH HARIAN TERHADAP KINERJA
Authors: Queer Wafa Almira , Waluyowati Nur Prima 
Published: August 2024

The objective of this research is to compare the performance of workers paid by the piece and the performance of those paid by hour. The research object is CV Albizzia Sinar Gemilang. This company applies two payment system to its workers. The respondents of this research are 83 production employees. The hypotheses of this research were tested using T test. This study finds different performance between workers paid by the piece and those paid by the hour. It advised that CV Albizzia Sinar Gemilang improve the performance of their employees and apply piece rate pay to all of its workers. Furthermore, the company is suggested to increase production supervision to improve the quality of its products.
Pengaruh Brand Image, Price, Dan Perceived Value Terhadap Purchase Intention Di Masa Pandemi Covid-19
Authors: Bawana Rachmad Aji , Mugiono Mugiono 
Published: August 2024

The objective of this research is to identify the effects of brand image, price, and perceived value on the purchase intention of Ultra Milk consumers in Greater Blitar region during the Covid-19 pandemic. The outbreak has caused negative impacts on the economy, so companies are required to run various marketing strategies in order to survive the business competition and increase consumers’ purchase intention. The respondents of this explanatory research are 88 Ultra Milk consumers in Greater Blitar region, selected using non-probability sampling method and purposive sampling technique. This study uses primary data harvested 5-point Likert-scaled questionnaires and analyzed using multiple linear regression in SPSS version 25. The results of the analysis have led to a finding that brand image, price, and perceived value significantly influence the purchase intention. Therefore, it suggested that the company should maintain the brand image, price, and the perceived value of Ultra Milk in order to gain higher purchase intention.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh Brand Image, Price, dan Perceived Value terhadap Purchase Intention konsumen Ultra Milk di wilayah Blitar Raya pada pandemi COVID – 19. Pandemi COVID – 19 berdampak negatif terhadap sektor ekonomi, perusahaan dituntut untuk melakukan berbagai strategi pemasaran agar dapat bertahan dalam persaingan bisnis dan menimbulkan Purchase Intention/minat beli konsumen. Jenis penelitian yang digunakan dalam penelitian ini adalah explanatory research. Jenis pengambilan sampel yaitu secara non-probabilitas dengan menggunakan teknik purposive sampling. Sampel terdiri atas 88 konsumen Ultra Milk di wilayah Blitar Raya sebagai responden. Data yang digunakan adalah data primer yang berasal dari kuisioner dengan menggunakan Skala Likert lima poin. Penelitian ini menggunakan metode analisis regresi linier berganda dengan bantuan software IBM SPSS versi 25. Hasil penelitian ini menunjukkan bahwa Brand Image, Price, dan Perceived Value berpengaruh secara signifikan terhadap Purchase Intention. Penelitian ini menyarankan agar perusahaan dapat mempertahankan dan meningkatkan Brand Image, Price, dan Perceived Value pada produk Ultra Milk untuk mendapatkan Purchase Intention.
PENGARUH ELECTRONIC WORD OF MOUTH TERHADAP LOYALITAS KONSUMEN YANG DIMEDIASI OLEH BRAND IMAGE DAN TRUST
Authors: Fadhlurrahman Muhammad Rafii’ , Sunaryo Sunaryo 
Published: August 2024

One of the uses of internet media in business applications is electronic commerce (e-commerce) or often also called electronic commerce. One of most used e-commerce by the Indonesian people is Tokopedia. This study aims to determine the effect of electronic word of mouth on consumer loyalty mediated by brand image and trust in Tokopedia users. The type of research used in this research is explanatory research. This study uses 100 samples, the sample is Tokopedia users in Malang City. The sampling technique used is a non-probability sampling technique , namely purposive sampling. Data analysis technique using SmartPLS 3.3. The results of this study indicate that electronic word of mouth has a direct positive and significant effect on consumer loyalty, brand image, and trust. Brand image and trust have a direct positive and significant influence on consumer loyalty. Brand image and trust have a positive and significant indirect influence in moderating electronic word of mouth on consumer loyalty.
Pengaruh Negara Asal, Persepsi Kualitas, Persepsi Nilai, dan Citra Merek Terhadap Brand Smartphone Vivo
Authors: Maulana Muhammad Shindid , Rohman Fatchur 
Published: August 2024

The goal of this research is to identify the effects of country of origin, perceived quality, perceived value, and brand image on customer’s intention to purchase Vivo smartphones. The effect to be studied is the direct effect of country of origin, perceived quality, perceived value, and brand image on purchase intention. This study is categorized as explanatory research since it tries to explain the causal relationship between variables through hypothesis testing. Using purposive sampling technique, 101 prospective smartphone buyers in Malang city were selected as the respondents. Here research instrument test and classical assumption test were incorporated. This study finds that country of origin does not motivate consumers to purchase, that perceived quality and perceived value encourage their purchase intention, and that brand image does not make them buy Vivo smartphones.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh Negara Asal, Persepsi Kualitas, Persepsi Nilai, dan Citra Merek terhadap Niat Beli pada brand smartphone Vivo. Pengaruh yang ingin diketahui adalah pengaruh langsung Negara Asal, Persepsi Kualitas, Persepsi Nilai, dan Citra Merek terhadap Niat Beli. Jenis penelitian ini adalah explanatory research yang menjelaskan hubungan kausal antara variabel-variabel melalui pengujian hipotesis. Penelitian ini menggunakan sampel sebanyak 101 responden. Pengambilan sampel menggunakan purposive sampling dengan karakteristik merupakan calon pembeli smartphone di Kota Malang. Alat uji yang digunakan adalah uji instrumen penelitian dan uji asumsi klasik. Dari hasil pengujian ini dapat disimpulkan bahwa Negara Asal tidak dapat mendorong konsumen untuk membeli. Persepsi Kualitas dan Persepsi Nilai dapat mendorong konsumen untuk membeli. Sedangkan Citra Merek tidak dapat mendorong konsumen untuk membeli brand smartphone Vivo.
Pengaruh Social Media Marketing Activities Terhadap Purchase Intention
Authors: Khairunnisa Nadhiva Ayu , Hendrawan Dimas 
Published: August 2024

This research was conducted by focusing on the identification of the effects of social media marketing activities on Instagram, which consists of entertainment, interaction, trendiness, customization, and word-of-mouth, on the intention of people in Jakarta to purchase the products of BASE, a skincare brand. The sample of this quantitative explanatory study consists of 134 respondents, which were selected form the population of people in Jakarta who know about the brand. The results of the analysis using a series of assessment performed in IBM SPSS 26 indicate that trendiness and word-of-mouth do not affect the purchase intention and that entertainment, interaction and customization positively contribute to the purchase intention.

Abstrak
Penelitian ini dilakukan dengan tujuan untuk berfokus pada mengetahui pengaruh dimensi Social Media Marketing Activities yakni, Entertainment, Interaction, Trendiness, Customization, dan Word of Mouth pada Instagram terhadap Purchase Intention skincare BASE di Jakarta. Jenis penelitian ini adalah penelitian eksplanatif menggunakan pendekatan kuantitatif. Penelitian ini menggunakan sampel sebanyak 134 responden yang berasal dari populasi orang yang mengetahui BASE di Jakarta. Alat analisis data yang digunakan adalah IBM SPSS 26. Hasil penelitian yang dilakukan menggunakan beberapa rangkaian pengujian menunjukkan hasil yang dapat disimpulkan bahwa Trendiness dan Word of Mouth tidak mampu untuk berkontribusi terhadap Purchase Intention. Entertainment, Interaction dan Customization mampu memiliki kontribusi yang positif terhadap Purchase Intention.
Strategi Komunikasi Pemasaran dan Strategi Bisnis Usaha dalam Meningkatkan Jumlah Konsumen
Authors: Al Kautsar Muhammad Hanief , Kurniawati Desi Tri 
Published: August 2024

This study aims to determine and analyze the marketing communication strategy and business strategy of Burger Cubit Indonesia (BUCIN) in increasing the number of consumers in Malang City. This type of research is descriptive qualitative with data collection techniques using interviews and documentation. Researchers used 4 informants, the informant is the owner of the culinary business and 3 others are customers of BUCIN located in Malang City. The data analysis carried out is using Miles and Huberman Analysis. The results showed that the marketing communication strategy applied by BUCIN is to use social media as an advertising medium, promo activities in the form of discounts and giveaways as promotional tools, as well as sadaqah and pop up kitchen activities as public relations tools. The business strategy applied by BUCIN is to sell mini-sized burger products with variants of archipelago flavors that are different from other burger outlets, open the Kebab Pokemon (KEPO) line and provide affordable prices.

Abstrak
Penelitian ini bertujuan untuk mengetahui dan menganalisis strategi komunikasi pemasaran dan strategi bisnis Usaha Burger Cubit Indonesia (BUCIN) dalam meningkatkan jumlah konsumen di Kota Malang. Jenis penelitian ini adalah deskriptif kualitatif dengan teknik pengambilan data menggunakan wawancara dan dokumentasi. Peneliti menggunakan 4 informan, informan tersebut ialah pemilik dari usaha kuliner tersebut dan 3 lainnya merupakan pelanggan dari BUCIN yang bertempat di Kota Malang. Analisis data yang dilakukan adalah menggunakan Analisis Miles dan Huberman.Hasil penelitian menunjukan bahwa strategi komunikasi pemasaran yang diterapkan oleh BUCIN adalah menggunakan social media sebagai media periklanan, kegiatan promo berupa diskon dan giveaway sebagai alat promosi, serta kegiatan sadaqah dan pop up kitchen sebagai alat hubungan masyarakat. Strategi bisnis yang diterapkan oleh BUCIN adalah menjual produk burger berukuran mini dan regular dengan varian rasa nusantara yang berbeda dengan gerai burger lainnya, membuka lini Kebab Pokemon (KEPO) serta memberikan harga yang terjangkau.
Pengaruh Word of Mouth, Brand Awareness, dan Store Atmosphere Terhadap Keputusan Pembelian
Authors: Prawira Bajra Dhira , Arif Moh. Erfan 
Published: August 2024

This study aims to determine the effect of word of mouth, brand awareness, and store atmosphere on purchasing decisions at NikiKopi Kepanjen. This type of research is explanatory research which explains the causal relationship between research variables through hypothesis testing. The sampling technique used nonprobability sampling and purposive sampling method. The population in the study were consumers who visited the NikiKopi Kepanjen shop. The sample size used was 150 respondents. Based on the results of testing the three hypotheses, it can be concluded that, the word of mouth variable has a positive and significant effect on the purchasing decision variable, the brand awareness variable has a direct positive effect on the purchasing decision variable, the store atmosphere variable has a positive effect on the purchasing decision variable. In this study, it can be concluded that the variables of word of mouth, brand awareness, and store atmosphere have a positive effect on purchasing decision.

Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh word of mouth, brand awareness, dan store atmosphere terhadap keputusan pembelian pada NikiKopi Kepanjen. Jenis penelitian yaitu penelitian eksplanatori yang menjelaskan hubungan kausal antar variabel penelitian melalui pengujian hipotesis. Teknik pengambilan sampel menggunakan nonprobability sampling dan metode purposive sampling. Populasi dalam penelitian merupakan konsumen yang mengunjungi kedai NikiKopi Kepanjen. Ukuran sampel yang digunakan adalah 150 responden. Analisis data menggunakan alat analisis IBM SPSS 25. Berdasarkan dari hasil pengujian terhadap ketiga hipotesis dapat disimpulkan bahwa, variabel word of mouth memiliki pengaruh positif dan signifikan terhadap variabel keputusan pembelian, variabel brand awareness memiliki pengaruh positif secara langsung terhadap variabel keputusan pembelian, variabel store atmosphere berpengaruh positif terhadap variable keputusan pembelian. Dalam penelitian ini dapat disimpulkan bahwa variabel word of mouth, brand awareness, dan store atmosphere berpengaruh secara positif variabel keputusan pembelian.