bluuebunny/crossref_metadata_2025_split huggingface dataset:
The structure of the Montgomery–Åsberg depression rating scale over the course of treatment for depression
Authors: Quilty Lena C. , Robinson Jennifer J. , Rolland Jean‐Pierre , Fruyt Filip De , Rouillon Frédéric , Bagby R. Michael 
Published: August 2013

AbstractThe Montgomery–Åsberg Depression Rating Scale (MADRS) is a widely used clinician‐rated measure of depressive severity. Empirical support for the factor structure of the MADRS is mixed; further, the comparison of MADRS scores within and between patients requires the demonstration of consistent instrument properties. The objective of the current investigation was to evaluate MADRS factor structure as well as MADRS factorial invariance across time and gender. The MADRS was administered to 821 depressed outpatients participating in a large‐scale effectiveness study of combined pharmacotherapy and psychotherapy for depression. Treatment outcome did not differ across treatment groups. Factor structure and invariance was evaluated via confirmatory factor analysis. A four‐factor model consisting of Sadness, Negative Thoughts, Detachment and Neurovegetative symptoms demonstrated a good fit to the data. This four‐factor structure was invariant across time and gender. A hierarchical model, in which these four factors served as indicators of a general depression factor, was also supported. A limitation of the current study is the lack of comprehensive characterization of patient clinical features; results need to be replicated in more severely depressed or treatment refractory patients. Overall, evidence supported the use of the MADRS total score as well as subscales focused on affective, cognitive, social and somatic aspects of depression in male and female outpatients. Copyright © 2013 John Wiley & Sons, Ltd.
Wingless/Wnt signaling in Drosophila: The pattern and the pathway
Authors: Bejsovec Amy 
Published: August 2013

SUMMARYWnt signaling generates pattern in all animal embryos, from flies and worms to humans, and promotes the undifferentiated, proliferative state critical for stem cells in adult tissues. Inappropriate Wnt pathway activation is the major cause of colorectal cancers, a leading cause of cancer death in humans. Although this pathway has been studied extensively for years, large gaps remain in our understanding of how it switches on and off, and how its activation changes cellular behaviors. Much of what is known about the pathway comes from genetic studies in Drosophila, where a single Wnt molecule, encoded by wingless (wg), directs an array of cell‐fate decisions similar to those made by the combined activities of all 19 Wnt family members in vertebrates. Although Wg specifies fate in many tissues, including the brain, limbs, and major organs, the fly embryonic epidermis has proven to be a very powerful system for dissecting pathway activity. It is a simple, accessible tissue, with a pattern that is highly sensitive to small changes in Wg pathway activity. This review discusses what we have learned about Wnt signaling from studying mutations that disrupt epidermal pattern in the fly embryo, highlights recent advances and controversies in the field, and sets these issues in the context of questions that remain about how this essential signaling pathway functions. Mol. Reprod. Dev. 80: 882–894, 2013. © 2013 Wiley Periodicals, Inc.
Post‐hatching development of in vitro bovine embryos from day 7 to 14 in vivo versus in vitro
Authors: Machado G.M. , Ferreira A.R. , Pivato I. , Fidelis A. , Spricigo J.F. , Paulini F. , Lucci C.M. , Franco M.M. , Dode M.A. 
Published: August 2013

SUMMARYThis study evaluates the post‐hatching development of in vitro‐produced (IVP) embryos until Day 14. On Day 7, IVP embryos were either transferred to recipient uteruses or placed in a post‐hatching development (PHD) system. As a control group, in vivo‐produced (IVV), Day‐7 embryos were also transferred to recipient uteruses. All groups were collected on Day 14 and were morphologically evaluated. Day‐7 and Day‐14 IVV and IVP embryos were used for quantification of eight genes (PLAC8, CD9, SLC2A1, SLC2A3, KRT8, SOD2, HSP1A1, and IFNT2) by reverse transcriptase qPCR. Day‐14 embryos from the PHD system were smaller (2.92 ± 0.45 mm) and had a lower embryonic disk diameter (0.14 ± 0.00 mm) than those produced by IVV (24.18 ± 3.71; 0.29 ± 0.03 mm, respectively) or IVP (19.06 ± 2.43; 0.28 ± 0.01 mm) culture and transferred to the uterus (P > 0.05). Day‐7 IVP embryos had a higher expression of the HSP1A1, SCL2A1, and SCL2A3 genes than IVV embryos. When these embryos were cultured in the uterus, no differences in gene expression were observed on Day 14. Conversely, Day‐14 IVP embryos cultured in the PHD system showed a higher expression of PLAC8, SOD2, and SLC2A3 genes. It is concluded that Day‐7 IVP embryos are different from IVV embryos in regards to gene expression, although exposure to the uterine environment during the elongation period allowed the IVP embryos to overcome this difference. In contrast, IVP embryos cultured in the PHD system were morphologically and molecularly different, being of poorer quality than those cultured in the uterus. Mol. Reprod. Dev. 80: 936–947, 2013. © 2013 Wiley Periodicals, Inc.
Association of musculoskeletal complaints and gliptin use: review of spontaneous reports
Authors: Tarapués Mónica , Cereza Gloria , Figueras Albert 
Published: August 2013

ABSTRACTPurposeGliptins are new oral antidiabetic drugs that increase insulin levels through dipeptidyl peptidase‐4 inhibition. Recently, the association of serious musculoskeletal (MSk) adverse effects with the gliptin use has been suggested. The aim is to describe and analyze the characteristics of spontaneous reports related to these adverse effects and gliptin use.MethodsAll spontaneous reports with MSk disorders associated with gliptins gathered in the Spanish pharmacovigilance database until May 2012 were described and analyzed using a case/non‐case method.ResultsGliptins were reported as the suspected drug in 332 cases: 208 involved sitagliptin, 115 vildagliptin, and nine saxagliptin. In 34 patients (10.2% of total reports with gliptins), MSk reactions were described (22 women [64.7%] and 12 men [35.3%]). Their mean age was 65.1 years; 41.2% were younger than 65 years. Only seven cases were serious, but the gliptin had to be withdrawn in 22 patients because of intolerance and/or persistence of MSk discomfort, and patients recovered after its discontinuation. A positive re‐challenge was observed in two cases. In seven cases, the patients were on previous chronic treatment with statins, and began to present MSk complaints shortly after starting a gliptin. The reporting odds ratio (ROR) for myalgia and arthralgia were strongly associated with gliptin use (ROR 1.96 [95% CI 1.12–3.43], p < 0.05 and ROR 2.69 [95% CI 1.38–5.24], p < 0.05, respectively).ConclusionsMusculoskeletal disorders are adverse reactions strongly associated with gliptins that, despite not being serious, may impair the treatment adherence in patients with type 2 diabetes. Future observational studies could confirm these findings. Copyright © 2013 John Wiley & Sons, Ltd.
Effects of inspiratory muscle training on lung volumes, respiratory muscle strength, and quality of life in patients with ataxia telangiectasia
Authors: Félix Erika , Gimenes Ana Cristina , Costa‐Carvalho Beatriz Tavares 
Published: August 2013

SummaryBackgroundAtaxia telangiectasia (AT) is a genetic syndrome caused by a mutation of chromosome 11. The clinical features are cerebellar ataxia, telangiectasia, and progressive loss of muscular coordination, including an inefficient cough secondary to progression of neurological disease.ObjectiveTo evaluate the effects of inspiratory muscle training (IMT) on ventilation, lung volume, dyspnoea, respiratory muscle strength, and quality of life in patients with AT.MethodsA longitudinal study was conducted with 11 AT patients and nine healthy volunteers. Ventilometry, subjective sensation of dyspnoea, maximal inspiratory pressure (MIP), maximal expiratory pressure (MEP), and quality of life were assessed before and after a 24‐week IMT program. The IMT load used was set at 60% of the MIP, and the training was performed for 20 min daily.ResultsPatients with AT had lower height and weight and also had lower respiratory muscle strength and lung volume compared with healthy volunteers. Furthermore, patients with AT showed a significant improvement when pre‐ and post‐IMT were compared for ventilatory pattern: Vt (476.5 ± 135 ml vs. 583.3 ± 66 ml, P = 0.015) and f (23.3 ± 6 rpm vs. 20.4 ± 4 rpm, P = 0.018), and VC (1,664 ± 463 ml/kg vs. 2,145 ± 750 ml/kg, P = 0.002). IMT also significantly improved the sensation of dyspnoea (median 0.5; minimum 0; maximum 1.0; P = 0.022) and respiratory muscle strength: MIP (−22.2 ± 2 cmH2O vs. −38 ± 9 cmH2O, P < 0.001) and MEP (29 ± 7 cmH2O vs. 40 ± 8 cmH2O, P = 0.001). The health and vitality domains of the SF‐36 also showed significant improvement (P = 0.009 and P = 0.014, respectively) post‐IMT.ConclusionIMT was effective in improving ventilatory pattern, lung volume, respiratory muscle strength, and the health and vitality domains for quality of life in patients with AT. IMT may be an effective adjunct therapy to drug treatment for patients with AT. Pediatr Pulmonol. 2014; 49:238–244. © 2013 Wiley Periodicals, Inc.
Prediction of glycan motifs using quantitative analysis of multi‐lectin binding: Motifs on MUC1 produced by cultured pancreatic cancer cells
Authors: McCarter Calvin , Kletter Doron , Tang Huiyuan , Partyka Katie , Ma Yinjiao , Singh Sudhir , Yadav Jessica , Bern Marshall , Haab Brian B. 
Published: August 2013

PurposeLectins are valuable tools for detecting specific glycans in biological samples, but the interpretation of the measurements can be ambiguous due to the complexities of lectin specificities. Here, we present an approach to improve the accuracy of interpretation by converting lectin measurements into quantitative predictions of the presence of various glycan motifs.Experimental designThe conversion relies on a database of analyzed glycan array data that provides information on the specificities of the lectins for each of the motifs. We tested the method using measurements of lectin binding to glycans on glycan arrays and then applied the method to predicting motifs on the protein mucin 1 (MUC1) expressed in eight different pancreatic cancer cell lines.ResultsThe combined measurements from several lectins were more accurate than individual measurements for predicting the presence or absence of motifs on arrayed glycans. The analysis of MUC1 revealed that each cell line expressed a unique pattern of glycoforms, and that the glycoforms significantly differed between MUC1 collected from conditioned media and MUC1 collected from cell lysates.Conclusions and clinical relevanceThis new method could provide more accurate analyses of glycans in biological sample and make the use of lectins more practical and effective for a broad range of researchers.
Evaluation of Different Conditions of Contact for Caprolactam Migration from Multilayer Polyamide Films into Food Simulants
Authors: Félix Juliana S. , Manzoli José Eduardo , Padula Marisa , Monteiro Magali 
Published: August 2013

ABSTRACTCaprolactam, the polyamide 6 (PA‐6) monomer, can migrate during food processing. At cooking temperatures, migration is accelerated and plastic components could degrade, giving off low molecular mass compounds, which can migrate into food. In this work, caprolactam migration from multilayer films containing PA‐6 for meat foodstuffs and cheese packaging was performed at contact conditions of 40°C/10 days and 100°C/30 min. The migration into water ranged from 0.89 to 1.22 mg/dm2 and 0.92 to 1.21 mg/dm2, into 3% acetic acid from 1.29 to 1.74 mg/dm2 and 1.13 to 1.62 mg/dm2 and into olive oil from 1.18 to 1.98 mg/dm2 and 0.50 to 0.80 mg/dm2 for films intended for meat foodstuffs for 10 days at 40°C and 30 min at 100°C, respectively. Among PA‐6 films used for cheese, caprolactam migration into water ranged from 0.17 to 0.91 mg/dm2 and 0.74 to 1.04 mg/dm2, into 3% acetic acid from 1.15 to 1.26 mg/dm2 and 1.11 to 1.37 mg/dm2 and into olive oil from 0.23 to 0.83 mg/dm2 and 0.37 to 0.56 mg/dm2 for 10 days at 40°C and 30 min at 100°C, respectively. Caprolactam migration evaluation into water and 3% acetic acid at 100°C/30 min could replace the need to apply the test at 40°C/10 days, since similar results were obtained under both conditions. In the case of PA‐6 for meat foodstuffs, caprolactam migration into olive oil was highly affected by different conditions of contact, showing values two to three times higher at 40°C/10 days than at 100°C/30 min. For cheese films, caprolactam migration into olive oil was higher at 40°C for 10 days. Copyright © 2013 John Wiley & Sons, Ltd.
Entrepreneurship in Emerging Markets: New Insights and Directions for Future Research
Authors: Alon Ilan , Rottig Daniel 
Published: August 2013

AbstractIn recent years, the entrepreneurship and emerging markets research streams have intersected. Emerging markets provide an opportunity to examine entrepreneurship in different contexts and forms. This article discusses the current literature that interconnects both research streams in general, comments on the contributions of the articles published in the special issue on entrepreneurship and emerging markets in particular, and sketches out future avenues for research. These contributions span several theoretical lenses, including institutional theory, internationalization theory, transaction cost economics, and the resource‐based view, as well as multiple geographic regions, including China as the largest emerging economy and other countries in East Asia, Central Asia, South Asia, Eastern Europe, Latin America, the Caribbean, and the Middle East. We weave a discussion of the extant literature and these contributions inside three key themes: Emerging Markets and China's Uniqueness, Entrepreneurship in Emerging Markets, and Future Research on Emerging Markets Entrepreneurship. © 2013 Wiley Periodicals, Inc.
Internationalization of Chinese Entrepreneurial Firms
Authors: Alon Ilan , Yeheskel Orly , Lerner Miri , Zhang Wenxian 
Published: August 2013

AbstractThis article uses the resource‐based and internationalization theories to explain the export behavior of Chinese entrepreneurial firms. Based on multiyear data on Chinese firms from the Global Entrepreneurship Monitor (GEM), we show that contextualized resource‐based theory can adequately explain some of the variation in export behavior among young Chinese firms. Exports by small Chinese firms are driven by the social and intellectual capital of the entrepreneur and their entrepreneurial proclivity, and the innovativeness/uniqueness of the product/offering. © 2013 Wiley Periodicals, Inc.
Students' Close Reading of Science Texts
Authors: Lapp Diane , Grant Maria , Moss Barbara , Johnson Kelly 
Published: August 2013

AbstractA question being asked by teachers across the country is, How can I teach students to acquire both the skills and knowledge demanded by the Common Core State Standards (CCSS) while simultaneously guiding them to read challenging texts such as those presented in Appendix B of the CCSS? Answering this invites the integration of ideas and the innovation of novel instructional plans. Illustrating close reading procedures in two classrooms‐ one 1st grade and one 5th grade‐ this article provides an answer by detailing the manner in which a student might approach a challenging science text using annotated reading, partner talk, text‐based questioning and reflective writing to build competence and a capacity for reading, writing, listening, and speaking about informational texts.
Role of Cholecystectomy and Lymph Node Dissection in Patients with T2 Gallbladder Cancer
Authors: Kim Dong Hyun , Kim Sung Hoon , Choi Gi Hong , Kang Chang Moo , Kim Kyung Sik , Choi Jin Sub , Lee Woo Jung 
Published: August 2013

AbstractBackgroundIncidental findings of gallbladder cancer (GBCA) have dramatically increased as an initial presentation of the disease because of the expansion of laparoscopic cholecystectomy. However, the optimal management of T2 GBCA remains at issue.MethodsWe compared our 10‐year experience with the consensus surgical strategy for T2 GBCA. Between January 2000 and December 2009, 70 patients at Severance Hospital, Yonsei University Health System, Seoul, Korea, underwent surgical treatment for GBCA stage T2. The medical records of 70 patients with T2 GBCA were retrospectively reviewed.ResultsRadical cholecystectomy was performed on only 32 (45.8 %) patients. In patients with T2 GBCA and positive lymph nodes (LN), the overall survival rate between cholecystectomy with LN dissection and radical cholecystectomy did not show a significant difference. Twenty patients experienced recurrence during the follow‐up period. Among the 11 patients who underwent cholecystectomy with liver resection, only 2 (18.2 %) patients had an intrahepatic recurrence. Of the 9 patients who underwent cholecystectomy without liver resection, 3 (33.3 %) patients had an intrahepatic recurrence. However, recurrences at the gallbladder bed occurred only in one and two patients, respectively, and were not significantly different between the two groups.ConclusionsThere was a large gap between clinical practice and treatment guidelines. Though relatively few patients enrolled in this study experienced recurrence, cholecystectomy and LN dissection without liver resection showed similar survival and recurrence patterns compared with those of radical cholecystectomy. To improve consistency between clinical practice and consensus guidelines, the role of limited resection for T2 lesions needs further evaluation.
Optimization, validation, and application of a real-time PCR protocol for quantification of viable bacterial cells in municipal sewage sludge and biosolids using reporter genes andEscherichia coli
Authors: van Frankenhuyzen Jessica K , Trevors Jack T , Flemming Cecily A , Lee Hung , Habash Marc B 
Published: August 2013

AbstractBiosolids result from treatment of sewage sludge to meet jurisdictional standards, including pathogen reduction. Once government regulations are met, materials can be applied to agricultural lands. Culture-based methods are used to enumerate pathogen indicator microorganisms but may underestimate cell densities, which is partly due to bacteria existing in a viable but non-culturable physiological state. Viable indicators can also be quantified by real-time polymerase chain reaction (qPCR) used with propidium monoazide (PMA), a dye that inhibits amplification of DNA found extracellularly or in dead cells. The objectives of this study were to test an optimized PMA-qPCR method for viable pathogen detection in wastewater solids and to validate it by comparing results to data obtained by conventional plating. Reporter genes from genetically marked Pseudomonas sp. UG14Lr and Agrobacterium tumefaciens 542 cells were spiked into samples of primary sludge, and anaerobically digested and Lystek-treated biosolids as cell-free DNA, dead cells, viable cells, and mixtures of live and dead cells, followed by DNA extraction with and without PMA, and qPCR. The protocol was then used for Escherichia coli quantification in the three matrices, and results compared to plate counts. PMA-qPCR selectively detected viable cells, while inhibiting signals from cell-free DNA and DNA found in membrane-compromised cells. PMA-qPCR detected 0.5–1 log unit more viable E. coli cells in both primary solids and dewatered biosolids than plate counts. No viable E. coli was found in Lystek-treated biosolids. These data suggest PMA-qPCR may more accurately estimate pathogen cell numbers than traditional culture methods.
Production, Biochemical Characterization and Detergents Application of Keratinase from the Marine Actinobacterium Actinoalloteichus sp. MA‐32
Authors: Manivasagan Panchanathan , Sivakumar Kannan , Gnanam Selvaraj , Venkatesan Jayachandran , Kim Se‐Kwon 
Published: August 2013

AbstractThe present study is the first report on poultry feathers as a novel, inexpensive substrate for the production of a thermo‐ and detergent stable keratinase from a marine actinobacterium belonging to the genus Actinoalloteichus. Medium composition and culture conditions for the keratinase production by Actinoalloteichus sp. MA‐32 were optimized using two statistical methods: Plackett–Burman design was applied to find the key ingredients and conditions for the best yield of enzyme production and central composite design used to optimize the concentration of the five significant variables: whole chicken feather, soy flour, MgSO4·7H2O, KH2PO4 and NaCl. The medium optimization resulted in a 19.30‐fold increase with a 31.99 % yield with a specific activity of 3842.57 U mg−1 and the molecular weight was estimated as 66 kDa. The enzyme was optimally active at pH 8–10 and temperature 50–60 °C and it was most stable up to pH 12 and 10–14 % of NaCl concentration. The enzyme activity was reduced when treated with Hg2+, Pb2+, Tween‐80, 1,10‐phenanthroline and EDTA and stimulated by Fe2+, Mg2+, Cu2+, Ca2+, Ni2+, Mn2+, SDS, ethoxylated (9.5EO) octylphenol, DMSO, sodium sulfite and β‐mercaptoethanol. The keratinase exhibited a significant stability and compatibility with most of the tested commercial laundry detergents, demonstrating its feasibility for inclusion in laundry detergent formulation. These results suggest that this extracellular keratinase may be a useful alternative and eco‐friendly route for handling the abundant amount of waste feathers or for applications in detergent formulation and other industrial processes.
Psychological interventions for adolescent psychosis: A pilot controlled trial in routine care
Authors: Browning S. , Corrigall R. , Garety P. , Emsley R. , Jolley S. 
Published: August 2013

AbstractPurposeEvidence for the recommendation to deliver Cognitive Behavioural Therapy (CBT) and Family Interventions (FI) to under-18s with psychosis derives from adult research, and no previous study has focused exclusively on an adolescent population. We evaluated adaptations of these therapies for adolescent inpatients with psychosis (CBTpA and FIpA), delivered as an adjunct to inpatient standard care (SC).Subjects and methodsThirty adolescent inpatients with psychotic symptoms on admission were sequentially allocated to receive CBTpA + SC (n = 10); FIpA + SC (n = 10) or SC alone (n = 10). Psychotic symptoms and functioning were measured at admission and discharge.ResultsGroup comparisons did not reach conventional significance, but effect sizes in this pilot study showed a promising impact of CBTpA compared to SC alone, in reducing symptoms (ES: d = 0.6), with smaller effect sizes for functioning (d = 0.2) and for FIpA (symptoms, d = 0.1 and functioning, d = 0.4). There was no advantage of either additional treatment in reducing length of stay, but self-report satisfaction ratings were higher for both psychological therapies.Discussion and conclusionsThe study is the first to focus on an exclusively adolescent population, using appropriately adapted therapy protocols. Findings suggest that the interventions are feasible, acceptable and helpful for adolescents with psychosis. Larger randomised controlled trials are now needed.