bluuebunny/crossref_metadata_2025_split huggingface dataset:
WIS‐neuromath enables versatile high throughput analyses of neuronal processes
Authors: Rishal Ida , Golani Ofra , Rajman Marek , Costa Barbara , Ben‐Yaakov Keren , Schoenmann Zohar , Yaron Avraham , Basri Ronen , Fainzilber Mike , Galun Meirav 
Published: October 2012

AbstractAutomated analyses of neuronal morphology are important for quantifying connectivity and circuitry in vivo, as well as in high content imaging of primary neuron cultures. The currently available tools for quantification of neuronal morphology either are highly expensive commercial packages or cannot provide automated image quantifications at single cell resolution. Here, we describe a new software package called WIS‐NeuroMath, which fills this gap and provides solutions for automated measurement of neuronal processes in both in vivo and in vitro preparations. Diverse image types can be analyzed without any preprocessing, enabling automated and accurate detection of neurites followed by their quantification in a number of application modules. A cell morphology module detects cell bodies and attached neurites, providing information on neurite length, number of branches, cell body area, and other parameters for each cell. A neurite length module provides a solution for images lacking cell bodies, such as tissue sections. Finally, a ganglion explant module quantifies outgrowth by identifying neurites at different distances from the ganglion. Quantification of a diverse series of preparations with WIS‐NeuroMath provided data that were well matched with parallel analyses of the same preparations in established software packages such as MetaXpress or NeuronJ. The capabilities of WIS‐NeuroMath are demonstrated in a range of applications, including in dissociated and explant cultures and histological analyses on thin and whole‐mount sections. WIS‐NeuroMath is freely available to academic users, providing a versatile and cost‐effective range of solutions for quantifying neurite growth, branching, regeneration, or degeneration under different experimental paradigms. © 2012 Wiley Periodicals, Inc. Develop Neurobiol, 2013
(Iminophosphorane)copper(I) Complexes as Highly Efficient Catalysts for 1,3‐Dipolar Cycloaddition of Azides with Terminal and 1‐Iodoalkynes in Water: One‐Pot Multi‐Component Reaction from Alkynes and in situ Generated Azides
Authors: García‐Álvarez Joaquín , Díez Josefina , Gimeno Jose , Suárez Francisco J. , Vincent Cristian 
Published: October 2012

AbstractTreatment of water‐soluble phosphanes PTA (1) and DAPTA (2) with an equimolecular amount of azides (RO)2P(=S)N3 (R = Et, Ph) leads to high‐yield formation of the N‐thiophosphorylated iminophosphorane derivatives 3b and 4a,b, respectively. The reaction of these new iminophosphorane ligands with [Cu(NCCH3)4][PF6] (in a 2:1 molar ratio) has been investigated. The resulting (iminophosphorane)copper(I) complexes 5b and 6a,b are efficient catalysts for the three‐component cycloaddition reaction (organic halide, NaN3 and terminal alkynes) in aqueous media to afford regioselectively, under mild and aerobic conditions according to “click laws”, 1,4‐disubstituted triazoles with a broad substrate scope and functional compatibility. The unprecedented application of the analogous (iminophosphorane)copper(I) catalyst 5a to a one‐pot three‐component reaction with 1‐iodoalkynes as an internal alkyne in aqueous medium is also reported. ESI‐MS analysis of 5a in water and DFT theoretical calculations [B3LYP/6‐31G(d)] have been carried out providing valuable insight into the actual active species responsible for catalytic activity in water.
Reduced Graphene Oxide|Carbon Ceramic Electrode Modified with CdS‐Hemoglobin as a Sensitive Hydrogen Peroxide Biosensor
Authors: Mohammad‐Rezaei Rahim , Razmi Habib 
Published: October 2012

AbstractA sensitive hydrogen peroxide (H2O2) biosensor was developed based on a reduced graphene oxide|carbon ceramic electrode (RGO|CCE) modified with cadmium sulfide‐hemoglobin (CdS‐Hb). The electron transfer kinetics of Hb were promoted due to the synergetic function of RGO and CdS nanoparticles. The transfer coefficient (α) and the heterogeneous electron transfer rate constant (ks) were calculated to be 0.54 and 2.6 s−1, respectively, indicating a great facilitation achieved in the electron transfer between Hb and the electrode surface. The biosensor showed a good linear response to the reduction of H2O2 over the concentration range of 2–240 µM with a detection limit of 0.24 µM (S/N=3) and a sensitivity of 1.056 µA µM−1 cm−2. The high surface coverage of the CdS‐Hb modified RGO|CCE (1.04×10−8 mol cm−2) and a smaller value of the apparent MichaelisMenten constant (0.24 mM) confirmed excellent loading of Hb and high affinity of the biosensor for hydrogen peroxide.
Immunoglobulin G4–related sclerosing disease of the paranasal sinus
Authors: Lindau Robert H. , Su Yungpo Bernard , Kobayashi Roger , Smith Russell B. 
Published: October 2012

BackgroundImmunoglobulin G4 (IgG4)–related sclerosing disease is a systemic disease characterized by extensive IgG4‐positive plasma cells and T‐lymphocyte infiltration of various organs. We present a case of a 69‐year‐old man with maxillary sinus IgG4 sclerosing disease, with orbital invasion treated with rituximab and dexamethasone pulse therapy. Surgery was used as well to debulk the disease and to obtain tissue for diagnosis.MethodsA PubMed search using the key phrase “IgG4‐related Sclerosing Disease” was performed. There were 304 different articles regarding the disease for a multitude of different organ sites. Of the 304 articles, there were 3 articles that reported this disease in the paranasal sinuses.ConclusionsIgG4‐related sclerosing disease is a rare entity in the head and neck. There are documented reports of steroid therapy for this disease, but the patient presented here demonstrated clinical progression of disease with steroids alone. The use of combination therapy of surgery, dexamethasone, and rituximab provided clinical improvement and stable disease determined by radiographic means. © 2012 Wiley Periodicals, Inc. Head Neck 35: E321–E324, 2013
Epstein–Barr virus‐induced epigenetic alterations following transient infection
Authors: Queen Krista J. , Shi Mingxia , Zhang Fangfang , Cvek Urska , Scott Rona S. 
Published: October 2012

AbstractEpstein–Barr virus (EBV) is a known tumor virus associated with an increasing array of malignancies; however, the association of the virus with certain malignancies is often erratic. To determine EBV's contributions to tumorigenesis in a setting of incomplete association, a transient model of infection was established where a clonal CCL185 carcinoma cell line infected with recombinant EBV was allowed to lose viral genomes by withdrawal of selection pressure. Global gene expression comparing EBV‐negative, transiently infected clones to uninfected controls identified expression changes in more than 1,000 genes. Among downregulated genes, several genes known to be deoxyribonucleic acid (DNA) methylated in cancer were identified including E‐cadherin and PYCARD. A cadherin switch, increased motility and enhanced cellular invasiveness present in EBV‐positive cells were retained after viral loss, indicating an epigenetic effect. Repression of PYCARD expression was a result of increased promoter CpG methylation, whereas loss of E‐cadherin expression after transient EBV infection did not correlate with increased DNA methylation of the E‐cadherin promoter. Rather, repression of E‐cadherin was consistent with the formation of a repressive chromatin state. Decreased histone 3 or 4 acetylation at the promoter and 5′ end of the E‐cadherin gene was observed in an EBV‐negative, transiently infected clone relative to the uninfected controls. These results suggest that EBV can stably alter gene expression in a heritable fashion in formerly infected cells, whereas its own contribution to the oncogenic process is masked.
Tobacco smoking and risk of multiple myeloma: A meta‐analysis of 40 observational studies
Authors: Psaltopoulou Theodora , Sergentanis Theodoros N. , Kanellias Nick , Kanavidis Prodromos , Terpos Evangelos , Dimopoulos Meletios A. 
Published: October 2012

AbstractThis meta‐analysis aims to quantitatively synthesize all available data on the association between tobacco smoking and multiple myeloma (MM) risk. Eligible studies were identified and pooled effect estimates (odds ratios and relative risks) were calculated regarding ever, current and former smoking. Separate analyses were performed on case–control and cohort studies, as well as on males and females. Meta‐regression analysis with percentage of males, mean age, years of smoking, pack‐years, cigarettes per day, years since quit and age at onset was performed. Forty articles were deemed eligible; of them 27 used a case–control design (4,625 cases and 21,591 controls) and 13 used a cohort design (2,228 incident cases among a total cohort size equal to 1,852,763 subjects). Ever smoking was not associated with MM risk (pooled effect estimate = 0.92, 95% confidence interval (CI): 0.85–1.00); similar results were obtained for current (pooled effect estimate = 0.87, 95% CI: 0.74–1.03) and former smoking (pooled effect estimate = 1.04, 95% CI: 0.96–1.13). Regarding ever smoking, the null association was reproducible upon cohort studies (pooled effect estimate = 1.01, 95%CI: 0.89–1.15), whereas the inverse association in case–control studies (pooled effect estimate = 0.87, 95% CI: 0.78–0.96) was particularly due to the bias‐prone hospital‐based ones. Meta‐regression analysis did not yield statistically significant results. In conclusion, MM does not seem to be associated with tobacco smoking. There is a need to further explore how molecular mechanisms are involved in the resistance of MM progenitor cells toward smoking.
Mortality among offspring of women diagnosed with cancer: A population‐based cohort study
Authors: Verkooijen Helena M. , Ang Joella X. , Liu Jenny , Czene Kamila , Salim Agus , Hartman Mikael 
Published: October 2012

AbstractOne in five cancers in women is diagnosed prior to and during a woman's fertile years. Our study evaluates mortality risks in offspring of mothers with history of cancer. From the Swedish Multi‐generation Register and the Cancer Register, we identified all 174,893 children whose mother had been diagnosed with cancer between 1958 and 2001. We categorized offspring into those born before (>1 year before), around (within 1 year before and after diagnosis) and after (>1 year after) their mother's cancer diagnosis and compared their risks of death (standardized mortality ratios, SMRs) and causes of death to the background population. Overall, offspring of mothers diagnosed with cancer had no increased mortality risk (SMR, 1.00; 95% confidence interval [CI], 0.97–1.03). Increased mortality risks were found in offspring of mothers with tobacco‐related cancers (head and neck, thoracic and cervical) (SMR, 1.23; 95% CI, 1.13–1.33), in children born around their mother's diagnosis (SMR, 1.66; 95% CI, 1.25–2.13) and in children born after their mother's hematopoietic cancer diagnosis (SMR, 2.07; 95% CI, 1.10–3.35). Compared to the background population, children born around their mother's diagnosis were more likely to die of congenital and perinatal conditions. Overall, offspring of women diagnosed with cancer were not at increased risk of death, except for certain subgroups. Timing of pregnancy in relation to diagnosis and cancer site modifies mortality risks in the offspring.
Recurrent inactivating mutations of ARID2 in non‐small cell lung carcinoma
Authors: Manceau Gilles , Letouzé Eric , Guichard Cécile , Didelot Audrey , Cazes Aurelie , Corté Hélène , Fabre Elizabeth , Pallier Karine , Imbeaud Sandrine , Le Pimpec‐Barthes Françoise , Zucman‐Rossi Jessica , Laurent‐Puig Pierre , Blons Hélène 
Published: October 2012

AbstractIn eukaryotic cells, DNA is packaged into chromatin and this compact storage in the nucleus promotes transcriptional repression of genes. Chromatin remodeling complexes such as the SWI/SNF complex are involved in making DNA accessible to transcription factors and thereby are implicated in the regulation of gene expression. Mutations and altered expression of chromatin remodeling complex genes have been described in cancer cells. Indeed, non‐small cell lung cancer cell lines have been shown to harbor mutations in SMARCA4 (BRG1), a member of the SWI/SNF complex, but evidence has been less clear in primary tumors. Recently, inactivating mutations in AT‐rich interaction domain 2 (ARID2) were found in liver cancer related to HCV infection and in melanoma. Here, we explored, using a genome‐wide strategy and subsequent sequencing of targeted genes, whether chromatin remodeling is implicated in primary lung adenocarcinoma. Two genes were individualized from the genome screening as homozygously deleted in a set of samples: JARID2 and ARID2. Subsequent analysis of the entire coding sequences showed that ARID2 loss‐of‐function mutations were found in 5% of nonsmall cell lung cancers, thereby constituting one of the most frequently mutated genes in this cancer type after TP53, KRAS, EGFR, CDKN2A and STK11.
Combined effects of water temperature and nutrients concentration on periphyton respiration – implications of global change
Authors: Rosa João , Ferreira Verónica , Canhoto Cristina , Graça Manuel A. S. 
Published: October 2012

AbstractWith the increase in global mean surface temperature predicted for the near future, stream water temperature will also increase. Simultaneously, water quality is likely to decrease (e.g., due to increases in nutrient and pollutant concentrations). The objective of this study was to evaluate the individual and combined effects of increases in water temperature and nutrients concentration on periphyton respiration, as a surrogate for stream metabolism. Stones naturally colonized with periphyton in an unpolluted mountain stream in Central Portugal were sampled seasonally over a year, and incubated in the laboratory under two water temperatures (ambient and 4°C elevated) and two nutrients concentration levels (ambient and ∼6× higher inorganic dissolved nitrogen, ∼2× higher soluble reactive phosphorous concentrations). Overall, increases in water temperature stimulated periphyton respiration to a larger extent than did increases in nutrients concentration. In spring, the simultaneous increase in water temperature and nutrients concentration stimulated periphyton respiration beyond expected from the individual effect of each factor. These results indicate that synergistic interactions between factors might occur under certain environmental conditions, suggesting that care should be taken when predicting the combined effect of changes in multiple factors from their individual effects. The observed stimulation of periphyton respiration promoted by increased temperature and nutrients concentration can lead to changes in streams carbon budgets, with a positive feedback for global warming, as more CO2 might be released to the atmosphere.
In vivo genotoxicity and cytotoxicity assessment of a novel quinoxalinone with trichomonacide activity
Authors: Rivera Norma , Rojas Marcela , Zepeda Armando , Malagón Filiberto , Arán Vicente J. , Marrero‐Ponce Yovani , Rivera Ernesto , Fortoul Teresa I. 
Published: October 2012

ABSTRACTThe compound VAM2‐6 (1‐methyl‐7‐nitro‐4‐(5‐(piperidin‐1‐yl)pentyl)‐3,4‐dihydroquinoxalin‐2(1H)‐one) has previously been shown to have an in vitro efficacy of 100% at a concentration of 100 µg ml–1 against Trichomonas vaginalis, a protozoon parasite that causes the sexually transmitted disease trichomoniasis. Because VAM2‐6 is a quinoxaline derivative and given the lack of studies on the genotoxic activity of this compound, the present study was undertaken to evaluate its ability to induce DNA damage in the peripheral blood of mice using single‐cell gel electrophoresis (SCGE or comet assay) and the micronucleus (MN) assay. Cell viability was assessed using a fluorochrome‐mediated viability test. The compound was tested on CD1 mice at 60, 40 and 10 mg kg–1 body weight administrated intraperitoneal (i.p.) in a single dose. Peripheral blood samples were collected 24 and 48 h after treatment. N‐Ethyl‐N‐nitrosourea (ENU) was used as a positive control for the comet and micronucleus assays. The results showed that i.p. VAM2‐6 induced single‐strand DNA breaks and increased the average number of micronuclei in the treated mice in a dose‐dependent manner at 60, 40 and 10 mg kg–1. Cell viability decreased at 24 h but recovered at 48 h for all three evaluated doses. Therefore, the chemical structure of VAM2‐6 should be modified to reduce its genotoxic potential. Copyright © 2012 John Wiley & Sons, Ltd.
Combined effects of metal contamination and temperature on the potworm Enchytraeus doerjesi (Oligochaeta)
Authors: Voua Otomo Patricks , Reinecke Sophie A. , Reinecke Adriaan J. 
Published: October 2012

ABSTRACTCadmium (Cd) and zinc (Zn) toxicity in Enchytraeus doerjesi after 28‐day exposure experiments was studied at 15, 20, and 25 °C. Cd lethal concentration (LC)50 decreased with increasing temperature (293.01 mg kg–1 at 15 °C, 261.62 mg kg–1 at 20 °C, 231.79 mg kg–1 at 25 °C). In contrast, Zn LC50 increased with increasing temperature (420.21 mg kg–1 at 15 °C and 518.42 mg kg–1 at 20 °C). At 25 °C, a Zn LC50 could not be computed owing to the lack of sufficient adult mortality (LC50 > 640 mg kg–1). The reproductive output at 20 and 25 °C was similar for each metal. Cd EC50 for reproduction increased between 15 and 20 °C (from 23.93 to 35.11 mg kg–1) and decreased between 20 °C and 25 °C (to 16.84 mg/kg). Zn EC50 increased dramatically from 15 °C (41.47 mg kg–1) to 20 °C (111.75 mg kg–1), and then slightly from 20 °C to 25 °C (112.17 mg kg–1). This indicated that metal–temperature effects on E. doerjesi may vary depending on the endpoint. The deleterious effects of Cd on the survival of E. doerjesi increased steadily with increasing temperature. With regard to reproduction, Cd toxicity could be exacerbated by extreme temperatures (15 and 25 °C) although conflicting pressures between high temperature and Cd toxicity may have prevented higher reproduction beyond 20 °C. The effects of Zn toxicity on survival decreased consistently with increasing temperature. The marginal increase in Zn EC50 between 20 and 25 °C may explain the lack of a significant change in reproduction between the two temperatures. Energy redistribution between the below organism and higher organization endpoints in response to metal contamination may further explain these results. Copyright © 2012 John Wiley & Sons, Ltd.
How do leadership motives affect informal and formal leadership emergence?
Authors: Luria Gil , Berson Yair 
Published: October 2012

SummaryTo what extent and in what ways do leadership motives of potential leaders predict their informal and formal leadership assignments? To address these questions, we conducted two studies in a military setting. In the first study (n = 215), we examined a mediated‐moderation model in which we hypothesized that the motivation to lead (MTL) of candidates to an elite unit would predict their teamwork behaviors and their tendency to emerge as leaders of their peers. We further hypothesized that cognitive ability would interact with MTL to predict teamwork behaviors and that teamwork behaviors would mediate the relationship between this interaction and leadership emergence. In Study 2, we followed up 60 candidates who were selected to the unit and examined whether MTL would predict the extent to which they achieved formal leadership roles. The findings of Study 1 supported the hypotheses included in the moderated mediation model. In Study 2, as expected, MTL predicted formal leadership emergence. We discuss several theoretical implications of these findings. Copyright © 2012 John Wiley & Sons, Ltd.
Groups, group actions and fields definable in first‐order topological structures
Authors: Wencel Roman 
Published: October 2012

AbstractGiven a group (G, ·), G⊆Mm, definable in a first‐order structure \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$\mathcal {M}=(M,\ldots )$\end{document} equipped with a dimension function and a topology satisfying certain natural conditions, we find a large open definable subset V⊆G and define a new topology τ on G with which (G, ·) becomes a topological group. Moreover, τ restricted to V coincides with the topology of V inherited from Mm. Likewise we topologize transitive group actions and fields definable in \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$\mathcal {M}$\end{document}. These results require a series of preparatory facts concerning dimension functions, some of which might be of independent interest.
Repeating blood cultures in neutropenic children with persistent fevers when the initial blood culture is negative
Authors: Rosenblum Jeremy , Lin Juan , Kim Mimi , Levy Adam S. 
Published: October 2012

AbstractBackgroundFebrile neutropenia is a common reason for the hospitalization of pediatric oncology patients. The initiation of antibiotics and the overall decline in rates of bacteremia, would predict a low yield of detection of bacteremia in repeated blood cultures. Despite little evidence supporting the utility of serial cultures, repeat culturing with fever persists.ProcedureTo determine the rate of follow‐up blood culture growth when the initial blood culture showed no bacterial growth and patient risk factors for this occurrence, we reviewed the records of oncology patients admitted to the Children's Hospital at Montefiore Pediatric Hematology/Oncology service for febrile neutropenia from 2004 to 2009.ResultsWe identified 457 febrile neutropenia episodes in 137 patients. The initial blood culture was positive in 84 episodes (18.4%). In 220 episodes comprising 105 patients, the initial blood culture was negative and a subsequent culture was obtained. In 24 episodes (10.9%), bacterial growth was detected in the repeat culture. Risk factors included a previous history of bacteremia and hospitalization for more than 48 hours prior to onset of fever.ConclusionsIn patients with febrile neutropenia, bacteremia is detected nearly twice as frequently in initial blood cultures than in repeat blood cultures obtained when the initial blood culture is negative. Despite an initial negative blood culture, bacteremia can be detected in more than 10% of episodes when a repeat blood culture is obtained. The risk more than doubles for patients with a previous history of bacteremia or hospitalized for more than 48 hours prior to the onset of fever. Pediatr Blood Cancer 2013; 60: 923–927. © 2012 Wiley Periodicals, Inc.
Luminescence characteristics of Sr1−xBaxSi2O2N2:Eu2+ phosphors for white light emitting diodes
Authors: Anoop G. , Cho I. H. , Suh D. W. , Yoo J. S. 
Published: October 2012

AbstractSr1−xBaxSi2O2N2:Eu2+ phosphors were synthesized using high temperature solid state reaction. The effect of Ba incorporation on the structural and luminescence characteristics of SrSi2O2N2:Eu2+ phosphors were studied. The phosphors were crystallized in triclinic crystal structure and the cell volume increases monotonically with Ba addition. The PL emission peak wavelength red shifts with Ba up to x = 0.50 beyond which no red shift is observed. The XPS analysis shows that nitrogen is being incorporated into the host lattice along with Ba addition up to x = 0.50. The as synthesized phosphors show high thermal stability. Phosphor converted light emitting diodes were realized using Sr1−xBaxSi2O2N2:Eu2+ phosphors (x = 0 and x = 0.40) showing luminance efficacies of 108 and 101 lm W−1. magnified imageThe CIE chromaticity coordinates of Sr1−xBaxSi2O2N2:Eu (x = 0 and x = 0.40) phosphors.