bluuebunny/crossref_metadata_2025_split huggingface dataset:
X-ray microtomography study of otic capsule deficiencies: three-dimensional modelling of the fissula ante fenestram
Authors: Lee J W , Sale P , Patel N P 
Published: August 2015

AbstractBackground:The postulated sites of perilymph fistulae involve otic capsule deficiencies, in particular, at the fissula ante fenestram. Histological studies have revealed this to be a channel extending from the middle ear, and becoming continuous with the inner ear medial to the anterior limit of the oval window. The relationship between a patent fissula and symptoms of perilymph fistula is contentious.Objective:The understanding of the anatomy of the fissula ante fenestram is incomplete. Histopathology is inherently destructive to the delicate ultrastructure of the middle and inner ear. Conversely, X-ray microtomography allows non-destructive examination of the otic capsule. In this study, we used X-ray microtomography to characterise the fissula ante fenestram.Materials and methods:We imaged cadaveric temporal bones with X-ray microtomography. We used the Avizo Fire (Visualization Science Group, Merignac Cedex, France) software to perform post-processing and image analysis.Results:Three-dimensional modelling of the fissula ante fenestram allowed stratification into four forms: rudimentary pit; partial fissula; complete occluded fissula; and complete patent fissula.Conclusion:X-ray microtomography showed that the fissula ante fenestram is present in various forms from rudimentary pit to complete deficiency of the otic capsule. This understanding may have implications for otologic surgery and clinical diagnosis of perilymph fistula.
American cars in Cotonou: culture in African entrepreneurship and the making of a globalising trade
Authors: Beuving Joost 
Published: August 2015

ABSTRACTTraders in Cotonou (Bénin), a prominent hub in the Euro–West African second-hand car trade, traditionally sold cars imported from Europe. Since the 2000s however, more and more cars are being imported from the US. Anthropological study of one group of entrepreneurs active in this new business, traders from Niger, reveals an African entrepreneurship at work that follows a distinct social pattern: traders are groomed in close kinship ties in West Africa and then develop new social ties with overseas migrants. Their trade thus becomes embedded in more globalised networks, yet at the same time it loosens and that works against profitable business. Close analysis of their careers reveals a cultural pattern that compels entrepreneurs to become traders, economic opportunity notwithstanding. Whether this is representative of Africa's changing place in the global economic order remains to be seen; however, this article suggests how culture in entrepreneurship may be key to understanding that.
China's example for Meles' Ethiopia: when development ‘models’ land
Authors: Fourie Elsje 
Published: August 2015

ABSTRACTThe past decade has seen the rapid rise of concepts such as the ‘China Model’ and the ‘Beijing Consensus’, yet more recent trends suggest a waning of their popularity. This article finds that the problem with the literature on the China model lies less with the concept itself than with a tendency to apply the term in an atheoretical and unempirical manner. From 2005 until at least 2012, Ethiopian elites from the upper echelons downwards were indeed engaged in a conscious and voluntary attempt to emulate aspects of China's perceived developmental successes. Drawing on interviews with 46 such elites, as well as on theories of lesson-drawing and cross-societal emulation, the study suggests that China may act as an example to countries seeking to achieve rapid modernisation and to navigate the perilous waters of political and economic globalisation. It is only by historicising and contextualising the ‘China Model’ within the older story of selective incorporation by certain ‘latecomer’ countries, however, that its true influence – and limits – can be understood.
Post-flood — Infectious Diseases in Mozambique
Authors: Kondo Hisayoshi , Seo Norimasa , Yasuda Tadashi , Hasizume Masahiro , Koido Yuichi , Ninomiya Norifumi , Yamamoto Yasuhiro 
Published: August 2015

AbstractIntroduction:The types of medical care required during a disaster are determined by variables such as the cycle and nature of the disaster. Following a flood, there exists the potential for transmission of water-borne diseases and for increased levels of endemic illnesses such as vector-borne diseases. Therefore, consideration of the situation of infectious diseases must be addressed when providing relief.The Japan Disaster Relief ( JDR) Medical Team was sent to Mozambique where a flood disaster occurred during January to March 2000. The team operated in the Hokwe area of the State of Gaza, in the mid-south of Mozambique where damage was the greatest.Methods:An epidemiological study was conducted. Information was collected from medical records by abstracting data at local medical facilities, interviewing in habitants and evacuees, and conducting analyses of water.Results:A total of 2,611 patients received medical care during the nine days. Infectious diseases were detected in 85% of all of patients, predominantly malaria, respiratory infectious diseases, and diarrhea. There was no outbreak of cholera or dysentery. Self-reports of the level of health decreased among the flood victims after the event. The incidence of malaria increased by four to five times over non-disaster periods, and the quality of drinking water deteriorated after the event.Conclusions:Both the number of patients and the incidence of endemic infectious diseases, such as malaria and diarrhea, increased following the flood. Also, there was a heightening of risk factors for infectious diseases such as an increase in population, deterioration of physical strength due to the shortage of food and the temporary living conditions for safety purposes, and turbid degeneration of drinking water. These findings support the hypotheses that there exists the potential for the increased transmission of water borne diseases and that there occurs increased levels of endemic illnesses during the post-flood period.
SENTIMENT AND MATERIALITY IN LATE VICTORIAN BOOK COLLECTING
Authors: Hanson David C. 
Published: August 2015

In the first half of the twentieth century, analytic bibliographers in Britain turned their attention to the systematic study of the nineteenth-century book. Developing their subject, they felt compelled to distance themselves from the Victorian book collector, who touched off a “suspicion . . . deeply ingrained in the mind of scholars and librarians” (Sadleir, “Development” 147). A new generation of bibliographers – Michael Sadleir, John Carter, and Graham Pollard – acknowledged that Victorian collecting had laid the foundations for the bibliographic study of books by “modern” (i.e., nineteenth-century) writers, as opposed to incunabula, the traditional focus of British book collecting. The contribution was regarded as fundamentally flawed, however, owing to a “sentimental element” in Victorian collecting (Carter and Pollard 101).
A scoping review to explore the suitability of interactive voice response to conduct automated performance measurement of the patient’s experience in primary care
Authors: Falconi Michael , Johnston Sharon , Hogg William 
Published: August 2015

IntroductionPractice-based performance measurement is fundamental for improvement and accountability in primary care. Traditional performance measurement of the patient’s experience is often too costly and cumbersome for most practices.Objective/MethodsThis scoping review explores the literature on the use of interactive voice response (IVR) telephone surveys to identify lessons for its use for collecting data on patient-reported outcome measures at the primary care practice level.ResultsThe literature suggests IVR could potentially increase the capacity to reach more representative patient samples and those traditionally most difficult to engage. There is potential for long-term cost effectiveness and significant decrease of the burden on practices involved in collecting patient survey data. Challenges such as low response rates, mode effects, high initial set-up costs and maintenance fees, are also reported and require careful attention.ConclusionThis review suggests IVR may be a feasible alternative to traditional patient data collection methods, which should be further explored.
CD163 interacts with TWEAK to regulate tissue regeneration after ischaemic injury
Authors: Akahori Hirokuni , Karmali Vinit , Polavarapu Rohini , Lyle Alicia N. , Weiss Daiana , Shin Eric , Husain Ahsan , Naqvi Nawazish , Van Dam Richard , Habib Anwer , Choi Cheol Ung , King Adrienne L. , Pachura Kimberly , Taylor W. Robert , Lefer David J. , Finn Aloke V. 
Published: August 2015

AbstractMacrophages are an essential component of the immune response to ischaemic injury and play an important role in promoting inflammation and its resolution, which is necessary for tissue repair. The type I transmembrane glycoprotein CD163 is exclusively expressed on macrophages, where it acts as a receptor for haemoglobin:haptoglobin complexes. An extracellular portion of CD163 circulates in the blood as a soluble protein, for which no physiological function has so far been described. Here we show that during ischaemia, soluble CD163 functions as a decoy receptor for TWEAK, a secreted pro-inflammatory cytokine of the tumour necrosis factor family, to regulate TWEAK-induced activation of canonical nuclear factor-κB (NF-κB) and Notch signalling necessary for myogenic progenitor cell proliferation. Mice with deletion of CD163 have transiently elevated levels of TWEAK, which stimulate muscle satellite cell proliferation and tissue regeneration in their ischaemic and non-ischaemic limbs. These results reveal a role for soluble CD163 in regulating muscle regeneration after ischaemic injury.
An epigenetic regulator emerges as microtubule minus-end binding and stabilizing factor in mitosis
Authors: Meunier Sylvain , Shvedunova Maria , Van Nguyen Nhuong , Avila Leonor , Vernos Isabelle , Akhtar Asifa 
Published: August 2015

AbstractThe evolutionary conserved NSL complex is a prominent epigenetic regulator controlling expression of thousands of genes. Here we uncover a novel function of the NSL complex members in mitosis. As the cell enters mitosis, KANSL1 and KANSL3 undergo a marked relocalisation from the chromatin to the mitotic spindle. By stabilizing microtubule minus ends in a RanGTP-dependent manner, they are essential for spindle assembly and chromosome segregation. Moreover, we identify KANSL3 as a microtubule minus-end-binding protein, revealing a new class of mitosis-specific microtubule minus-end regulators. By adopting distinct functions in interphase and mitosis, KANSL proteins provide a link to coordinate the tasks of faithful expression and inheritance of the genome during different phases of the cell cycle.
Greater serum carotenoid levels associated with lower prevalence of nonalcoholic fatty liver disease in Chinese adults
Authors: Cao Yi , Wang Cheng , Liu Jun , Liu Zhao-min , Ling Wen-hua , Chen Yu-ming 
Published: August 2015

AbstractPrevious studies have suggested that serum carotenoids may be inversely associated with liver injury, but limited data are available from population-based studies. We examined the relationship between serum carotenoid levels and the prevalence of nonalcoholic fatty liver disease (NAFLD) in Chinese adults. A total of 2935 participants aged 40–75 years were involved in this community-based cross-sectional study. General information, lifestyle factors, serum levels of carotenoid and the presence and degree of NAFLD were determined. After adjusting for potential covariates, we observed a dose-dependent inverse association between NAFLD risk and each individual serum carotenoid and total carotenoids (all p-values < 0.001). The ORs of NAFLD for the highest (vs. lowest) quartile were 0.44 (95% CI 0.35, 0.56) for α-carotene, 0.32 (95% CI 0.25, 0.41) for β-carotene, 0.62 (95% CI 0.49, 0.79) for β-cryptoxanthin, 0.54 (95% CI 0.42, 0.68) for lycopene, 0.56 (95% CI 0.44, 0.72) for lutein + zeaxanthin and 0.41 (95% CI 0.32, 0.53) for total carotenoids. Higher levels of α-carotene, β-carotene, lutein + zeaxanthin and total carotenoids were significantly associated with a decrease in the degree of NAFLD (p-trend: < 0.001 to 0.003). Serum carotenoids are inversely associated with prevalence of NAFLD in middle aged and elderly Chinese.
Simultaneous fingerprint and high-wavenumber fiber-optic Raman spectroscopy improves in vivo diagnosis of esophageal squamous cell carcinoma at endoscopy
Authors: Wang Jianfeng , Lin Kan , Zheng Wei , Yu Ho Khek , Teh Ming , Guan Yeoh Khay , Huang Zhiwei 
Published: August 2015

AbstractThis work aims to evaluate clinical value of a fiber-optic Raman spectroscopy technique developed for in vivo diagnosis of esophageal squamous cell carcinoma (ESCC) during clinical endoscopy. We have developed a rapid fiber-optic Raman endoscopic system capable of simultaneously acquiring both fingerprint (FP)(800–1800 cm−1) and high-wavenumber (HW)(2800–3600 cm−1) Raman spectra from esophageal tissue in vivo. A total of 1172 in vivo FP/HW Raman spectra were acquired from 48 esophageal patients undergoing endoscopic examination. The total Raman dataset was split into two parts: 80% for training; while 20% for testing. Partial least squares-discriminant analysis (PLS-DA) and leave-one patient-out, cross validation (LOPCV) were implemented on training dataset to develop diagnostic algorithms for tissue classification. PLS-DA-LOPCV shows that simultaneous FP/HW Raman spectroscopy on training dataset provides a diagnostic sensitivity of 97.0% and specificity of 97.4% for ESCC classification. Further, the diagnostic algorithm applied to the independent testing dataset based on simultaneous FP/HW Raman technique gives a predictive diagnostic sensitivity of 92.7% and specificity of 93.6% for ESCC identification, which is superior to either FP or HW Raman technique alone. This work demonstrates that the simultaneous FP/HW fiber-optic Raman spectroscopy technique improves real-time in vivo diagnosis of esophageal neoplasia at endoscopy.
Structural identification of the myo-inositol 1,4,5-trisphosphate-binding domain in rat brain inositol 1,4,5-trisphosphate 3-kinase
Authors: TOGASHI Shinji , TAKAZAWA Kazunaga , ENDO Toyoshi , ERNEUX Christophe , ONAYA Toshimasa 
Published: August 2015

A series of key amino acids involved in Ins(1,4,5)P3 (InsP3) binding and catalytic activity of rat brain InsP3 3-kinase has been identified. The catalytic domain is at the C-terminal end and restricted to a maximum of 275 amino acids [Takazawa and Erneux (1991) Biochem. J. 280, 125–129]. In this study, newly prepared 5′-deletion and site-directed mutants have been compared both for InsP3 binding and InsP3 3-kinase activity. When the protein was expressed from L259 to R459, the activity was lost but InsP3 binding was conserved. Another deletion mutant that had lost only four amino acids after L259 had lost InsP3 binding, and this finding suggests that these residues (i.e. L259DCK262) are involved in InsP3 binding. To further support the data, we have produced two mutants by site-directed mutagenesis on residues C261 and K262. The two new enzymes were designated M4 (C261S) and M5 (K262A). M4 showed similar Vmax and Km values for InsP3 and ATP to wild-type enzyme. In contrast, M5 was totally inactive but had kept the ability to bind to calmodulin–Sepharose. C-terminal deletion mutants that had lost five, seven or nine amino acids showed a large decrease in InsP3 binding and InsP3 3-kinase activity. One mutant that had lost five amino acids (M2) was purified to apparent homogeneity: Km values for both substrates appeared unchanged but Vmax was decreased approx. 40-fold compared with the wild-type enzyme. The results indicate that (1) a positively charged amino acid residue K262 is essential for InsP3 binding and (2) amino acids at the C-terminal end of the protein are necessary to act as a catalyst in the InsP3 3-kinase reaction.
Regulation of the biosynthesis of 4,7,10,13,16-docosapentaenoic acid
Authors: MOHAMMED B. Selma , LUTHRIA Devanand L. , BAKOUSHEVA Svetla P. , SPRECHER Howard 
Published: August 2015

It is now established that fatty acid 7,10,13,16-22:4 is metabolized into 4,7,10,13,16-22:5 as follows: 7,10,13,16-22:4 → 9,12,15,18-24:4 → 6,9,12,15,18-24:5 → 4,7,10,13,16-22:5. Neither C24 fatty acid was esterified to 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) by microsomes, whereas the rates of esterification of 4,7,10,13,16-22:5, 7,10,13,16-22:4 and 5,8,11,14-20:4 were respectively 135, 18 and 160 nmol/min per mg of microsomal protein. About four times as much acid-soluble radioactivity was produced when peroxisomes were incubated with [3-14C]9,12,15,18-24:4 compared with 6,9,12,15,18-24:5. Only [1-14C]7,10,13,16-22:4 accumulated when [3-14C]9,12,15,18-24:4 was the substrate, but both 4,7,10,13,16-22:5 and 2-trans-4,7,10,13,16-22:6 were produced from [3-14C]6,9,12,15,18-24:5. When the two C24 fatty acids were incubated with peroxisomes, microsomes and 1-acyl-GPC there was a decrease in the production of acid-soluble radioactivity from [3-14C]6,9,12,15,18-24:5, but not from [3-14C]9,12,15,18-24:4. The preferential fate of [1-14C]4,7,10,13,16-22:5, when it was produced, was to move out of peroxisomes for esterification into the acceptor, whereas only small amounts of 7,10,13,16-22:4 were esterified. By using 2H-labelled 9,12,15,18-24:4 it was shown that, when 7,10,13,16-22:4 was produced, its primary metabolic fate was degradation to yield esterified arachidonate. Collectively, the results show that an inverse relationship exists between rates of peroxisomal β-oxidation and of esterification into 1-acyl-GPC by microsomes. Most importantly, when a fatty acid is produced with its first double bond at position 4, it preferentially moves out of peroxisomes for esterification to 1-acyl-GPC by microsomes, rather than being degraded further via a cycle of β-oxidation that requires NADPH-dependent 2,4-dienoyl-CoA reductase.
Heparin inhibits the binding of basic fibroblast growth factor to cultured human aortic smooth-muscle cells
Authors: BONO Françoise , RIGON Patrice , LAMARCHE Isabelle , SAVI Pierre , SALEL Véronique , HERBERT Jean-Marc 
Published: August 2015

Basic fibroblast growth factor (bFGF) and its specific receptors have diverse roles on a variety of cell types, such as the induction of vascular smooth-muscle cell proliferation which contributes to restenosis after coronary balloon angioplasty. bFGF is also known to interact with heparan sulphate proteoglycans present on the cell surface or in the extracellular matrix. In this study, the binding of 125I-bFGF to human aortic smooth-muscle cells was investigated. 125I-bFGF binding to these cells was reversible and saturable. Scatchard analysis revealed the presence of two distinct binding sites: a high-affinity receptor (Kd = 38±7 pM; 1480±220 sites/cell) and a low-affinity non-saturable binding site (Kd= 8.0±2.0 nM). Pretreatment of the cells with heparinase resulted in a large reduction of 125I-bFGF binding to its low-affinity receptors, suggesting that they are heparin-like molecules. The specificity of the low- and high-affinity binding sites for bFGF was determined with acidic FGF, platelet-derived growth factor-BB and epidermal growth factor, which did not compete for 125I-bFGF binding. Expression of FGF receptor isoforms analysed by reverse transcriptase-PCR revealed the presence of only the type-1 receptor. Binding to low-affinity binding sites was antagonized by heparin, suramin, protamine sulphate and platelet factor 4. Unexpectedly, these molecules also reduced the binding of 125I-bFGF to its high-affinity sites. Consistent with these results, heparin, suramin, protamine sulphate and platelet factor 4 inhibited bFGF-induced proliferation of human aortic smooth-muscle cells. Heparin abrogated bFGF-induced release of tissue-type plasminogen activator by these cells. These observations suggest that the interaction of bFGF with human aortic smooth-muscle cells is different from that described for other cells such as endothelial cells, in which heparin acts as a potentiating factor of the mitogenic activity of bFGF.
The integrity of the RRGDL sequence of the proprotein convertase PC1 is critical for its zymogen and C-terminal processing and for its cellular trafficking
Authors: LUSSON Juliette , BENJANNET Suzanne , HAMELIN Josée , SAVARIA Diane , CHRÉTIEN Michel , SEIDAH Nabil G. 
Published: August 2015

In order to define the functional importance of the conserved RRGDL motif in the P-domain of the mammalian proprotein convertases (PCs) we generated and cellularly expressed three mutant PC1 vaccinia-virus (VV) recombinants: ARGDL-PC1, RAGDL-PC1 and RRGEL-PC1. Functionally, these mutants caused a decreased level of processing of pro-opiomelanocortin (POMC) into β-lipotropic pituitary hormone (β-LPH), especially in the constitutively secreting BSC40 cells. Pulse–chase analyses demonstrated that, in part, this effect was due to both an increased degradation of the mutant PC1s within the endoplasmic reticulum and to a diminished level of zymogen processing in the same compartment. In addition, within cells containing secretory granules such as PC12 and GH4C1 cells, such mutations prevented the C-terminal auto-processing of PC1 into the fully mature 66 kDa form stored in the secretory granules of regulated cells. Since the 66 kDa PC1 is the most active form of the enzyme, it is proposed that the RRGDL sequence is critical for the generation of maximal intracellular PC1 activity. In regulated cells, co-expression of POMC with PC1 or its mutants together with the general PC inhibitor α1-antitrypsin Portland (α1-PDX), which acts primarily within the constitutive secretory pathway, demonstrated that the latter completely inhibited the formation of β-LPH by PC1 mutants, whereas it only partially inhibited the ability of wild-type PC1 to process POMC. This suggests that RRGDL mutations prevent PC1 from entering secretory granules and hence the formation of the 66 kDa PC1, and result in the mis-sorting of PC1 mutants towards the constitutive secretory pathway. This conclusion was further supported by immunocytochemical data demonstrating that RRGDL mutants exhibit an intracellular localization pattern different from that of the granule-associated wild-type PC1, but similar to that of the Golgi-localized convertase PC5-B.