bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting of tumor endothelial cells combining 2 Gy/day of X‐ray with Everolimus is the effective modality for overcoming clinically relevant radioresistant tumors
Authors: Kuwahara Yoshikazu , Mori Miyuki , Kitahara Shuji , Fukumoto Motoi , Ezaki Taichi , Mori Shiro , Echigo Seishi , Ohkubo Yasuhito , Fukumoto Manabu 
Published: January 2014

AbstractRadiotherapy is widely used to treat cancer because it has the advantage of physically and functionally conserving the affected organ. To improve radiotherapy and investigate the molecular mechanisms of cellular radioresistance, we established a clinically relevant radioresistant (CRR) cell line, SAS‐R, from SAS cells. SAS‐R cells continue to proliferate when exposed to fractionated radiation (FR) of 2 Gy/day for more than 30 days in vitro. A xenograft tumor model of SAS‐R was also resistant to 2 Gy/day of X‐rays for 30 days. The density of blood vessels in SAS‐R tumors was higher than in SAS tumors. Everolimus, a mammalian target of rapamycin (mTOR) inhibitor, sensitized microvascular endothelial cells to radiation, but failed to radiosensitize SAS and SAS‐R cells in vitro. Everolimus with FR markedly reduced SAS and SAS‐R tumor volumes. Additionally, the apoptosis of endothelial cells (ECs) increased in SAS‐R tumor tissues when both Everolimus and radiation were administered. Both CD34‐positive and tomato lectin‐positive blood vessel densities in SAS‐R tumor tissues decreased remarkably after the Everolimus and radiation treatment. Everolimus‐induced apoptosis of vascular ECs in response to radiation was also followed by thrombus formation that leads to tumor necrosis. We conclude that FR combined with Everolimus may be an effective modality to overcome radioresistant tumors via targeting tumor ECs.
Anticancer Potential of (Pentamethylcyclopentadienyl)chloridoiridium(III) Complexes Bearing κP and κP,κS‐Coordinated Ph2PCH2CH2CH2S(O)xPh (x=0–2) Ligands
Authors: Ludwig Gerd , Ranđelović Ivan , Maksimović‐Ivanić Danijela , Mijatović Sanja , Bulatović Mirna Z. , Miljković Djordje , Korb Marcus , Lang Heinrich , Steinborn Dirk , Kaluđerović Goran N. 
Published: January 2014

AbstractIridium(III) complexes of the type [Ir(η5‐C5Me5)Cl2{Ph2PCH2CH2CH2S(O)xPh‐κP}] (x=0–2; 1–3) and [Ir(η5‐C5Me5)Cl{Ph2PCH2CH2CH2S(O)xPh‐κP,κS}][PF6] (x=0–1; 4 and 5) with 3‐(diphenylphosphino)propyl phenyl sulfide, sulfoxide, and sulfone ligands Ph2PCH2CH2CH2S(O)xPh were designed, synthesized, and characterized fully, including X‐ray diffraction analyses for complexes 3 and 4. In vitro studies against human thyroid carcinoma (8505C), submandibular carcinoma (A253), breast adenocarcinoma (MCF‐7), colon adenocarcinoma (SW480), and melanoma (518A2) cell lines provided evidence for the high biological potential of the neutral and cationic iridium(III) complexes. Neutral iridium(III) complex 5 proved to be the most active, with IC50 values up to about 0.1 μM, representing activities of up to one order of magnitude higher than cisplatin. Using 8505C cells, apoptosis was shown to be the main mechanism through which complex 5 exerts its tumoricidal action. The described iridium(III) complexes represent potential leads in the search for novel metal‐based anticancer agents.
Iodinated AlIII‐Based Phthalocyanines are Promising Sensitizers for Dye‐Sensitized Solar Cells; A Theoretical Comparison Between ZnII, MgII, and AlIII‐Based Phthalocyanine Sensitizers
Authors: Yang Li‐Na , Sun Zhu‐Zhu , Chen Shi‐Lu , Li Ze‐Sheng 
Published: January 2014

AbstractTo design efficient dyes for dye‐sensitized solar cells (DSSCs), using a Zn‐coordinated phthalocyanine (TT7) as the prototype, a series of phthalocyanine dyes (Pcs) with different metal ions and peripheral/axial groups have been investigated by means of density functional theory (DFT) and time‐dependent DFT (TDDFT) methods. Computational results show that the iodinated Al‐based dye with a peripheral amino group (Al‐I‐NH2‐Pc) exhibits the largest redshift in the maximum absorbance (λmax). In addition, Al‐based dyes have appropriate energy‐level arrangements of frontier orbitals to keep excellent balance between electron injection and regeneration of oxidized dyes. Further, it has been found that the intermolecular π‐staking interaction in Al‐I‐Pc molecules is weaker than the other metal‐based Pcs, which may effectively reduce dye aggregation on the semi‐conductor surface. All these results suggest iodinated Al‐based Pcs (Al‐I‐Pcs) to be potentially promising sensitizers in DSSCs.
Peptide‐Nanofiber‐Supported Palladium Nanoparticles as an Efficient Catalyst for the Removal of N‐Terminus Protecting Groups
Authors: Maity Indrajit , Manna Manoj K. , Rasale Dnyaneshwar B. , Das Apurba K. 
Published: January 2014

AbstractSonication‐induced tryptophan‐ and tyrosine‐based peptide bolaamphiphile nanofibers have been used to synthesize and stabilize Pd nanoparticles under physiological conditions. The peptide bolaamphiphile self‐assembly process has been thoroughly studied by using several spectroscopic and microscopic techniques. The stiffness of the soft hydrogel matrix was measured by an oscillatory rheological experiment. FTIR and circular dichroism (CD) experiments revealed a hydrogen‐bonded β‐sheet conformation of peptide bolaamphiphile molecules in a gel‐phase medium. The π–π stacking interactions also played a crucial role in the self‐assembly process, which was confirmed by fluorescence spectroscopy. Electron (SEM and TEM) and atomic force microscopy (AFM) studies showed that the peptide bolaamphiphile molecules self‐assemble into nanofibrillar structures. Pd nanoparticles were synthesized in the hydrogel matrix in which redox‐active tryptophan and tyrosine residues reduce the metal ions to metal nanoparticles. The size of the Pd nanoparticles are in the range of 3–9 nm, and are stabilized by peptide nanofibers. The peptide‐nanofiber‐supported Pd nanoparticles have shown effective catalytic activity for the removal of N‐terminus protecting groups of amino acids and peptides.
Synthesis and Optical Properties of Imidazole‐Based Fluorophores having High Quantum Yields
Authors: Lessi Marco , Manzini Chiara , Minei Pierpaolo , Perego Luca A. , Bloino Julien , Egidi Franco , Barone Vincenzo , Pucci Andrea , Bellina Fabio 
Published: January 2014

AbstractA series of 1,4‐phenylene‐spaced bis‐imidazoles with fluorescence quantum yields up to 0.90 and large Stokes shifts have been designed and synthesized using recently developed regioselective direct CH arylation protocols. All the fluorophores show a bright blue‐green emission that is well retained in the solid state. DFT calculations attributed their excellent luminescence properties to the significant planarization of the molecules in the equilibrium structures of their excited electronic states.
Preliminary Evidence for a Nexus between Rumination, Behavioural Avoidance, Motive Satisfaction and Depression
Authors: Brockmeyer Timo , Holtforth Martin Grosse , Krieger Tobias , Altenstein David , Doerig Nadja , Zimmermann Johannes , Backenstrass Matthias , Friederich Hans‐Christoph , Bents Hinrich 
Published: January 2014

The present study tested a theoretically derived link between rumination and depressive symptoms through behavioural avoidance and reduced motive satisfaction as a key aspect of positive reinforcement. Rumination, behavioural avoidance, motive satisfaction and levels of depression were assessed via self‐report measures in a clinical sample of 160 patients with major depressive disorder. Path analysis‐based mediation analysis was used to estimate the direct and indirect effects as proposed by the theoretical model. Operating in serial, behavioural avoidance and motive satisfaction partially mediated the association between rumination and depressive symptoms, irrespective of gender, medication and co‐morbid anxiety disorders. This is the first study investigating the associations between behavioural avoidance, rumination and depression in a clinical sample of depressed patients. The findings are in line with an understanding of rumination in depression as also serving an avoidance function. Copyright © 2014 John Wiley & Sons, Ltd.Key Practitioner Message

Rumination, avoidance, motive satisfaction and levels of depressive symptoms were examined in a clinical sample of 160 outpatients with major depressive disorder.
Path analysis‐based mediation analysis revealed that, operating in serial, avoidance and motive satisfaction partially mediated the link between rumination and levels of depressive symptoms.
Findings support an understanding of rumination in depression as serving an avoidance function.
Burden of diabetic foot disorders, guidelines for management and disparities in implementation in Europe: a systematic literature review
Authors: van Acker Kristien , Léger Philippe , Hartemann Agnes , Chawla Abhineet , Siddiqui Mohd Kashif 
Published: January 2014

SummaryThe study aimed to assess the economic and quality of life burden of diabetic foot disorders and to identify disparities in the recommendations from guidelines and the current clinical practice across the EU5 (Spain, Italy, France, UK and Germany) countries. Literature search of electronic databases (MEDLINE®, Embase® and Cochrane Database of Systematic Reviews) was undertaken. English language studies investigating economic and resource burden, quality of life and management of diabetic foot disease in the EU5 countries were included. Additionally, websites were screened for guidelines and current management practices in diabetic foot complication in EU5. Diabetic foot complications accounted for a total annual cost of €509m in the UK and €430 per diabetic patient in Germany, during 2001. The cost of diabetic foot complications increased with disease severity, with hospitalizations (41%) and amputation (9%) incurring 50% of the cost. Medical devices (orthopaedic shoes, shoe lifts and walking aids) were the most frequently utilized resources. Patients with diabetic foot complications experienced worsened quality of life, especially in those undergoing amputations and with non‐healed ulcers or recurrent ulcers. Although guidelines advocate the use of multidisciplinary foot care teams, the utilization of multidisciplinary foot care teams was suboptimal. We conclude that diabetic foot disorders demonstrated substantial economic burden and have detrimental effect on quality of life, with more impairment in physical domain. Implementation of the guidelines and set‐up of multidisciplinary clinics for holistic management of the diabetic foot disorders varies across Europe and remains suboptimal. Hence, guidelines need to be reinforced to prevent diabetic foot complications and to achieve limb salvage if complications are unpreventable. Copyright © 2014 John Wiley & Sons, Ltd.
CuII‐Templated Threading of a Bis‐amide‐tris‐amine Macrocycle by Substituted 2,2′‐Bipyridyl Derivatives Assisted by Strong π–π Stacking and Second‐Sphere H‐Bonding Interactions
Authors: Saha Subrata , Santra Saikat , Ghosh Pradyut 
Published: January 2014

AbstractDerivatives of 5,5′‐substituted 2,2′‐bipyridine (BPy) ligands with bromo‐terminated aliphatic side chains with ester functionalities (BPy5Br), hydroxy‐terminated aliphatic side chains with amide functionalities (BPy5OH), and benzyl‐terminated side chains with amide functionalities (BPy5Bnz) were synthesized. The single‐crystal X‐ray structure analysis of the axle BPy5Br shows that it has a linear rodlike structure with a length of 15.75 Å. The above three ligands along with a methyl‐substituted BPy ligand (BPy5Me) are explored as axles for CuII‐templated threading of a bis‐amide‐tris‐amine macrocycle wheel (MC). High‐yield (70–80 %) syntheses of the [2]pseudorotaxanes PRT1, PRT2, PRT3, and PRT4 are obtained by reaction of MC with the axles BPy5Me, BPy5Br, BPy5OH, and BPy5Bnz, respectively, in the presence of CuII ions. These complexes were characterized by ESI‐MS, UV/Vis and EPR spectroscopy, and single‐crystal X‐ray diffraction studies. The solution‐state binding stoichiometry and the association constants for complex formation between MC–Cu2+ and the axles were studied by UV/Vis absorption titrations. Sigma‐fit curves indicate 1:1 stoichiometry, and the association constants range from 1.2 × 103 to 2.6 × 103 M–1. Single‐crystal X‐ray structure analysis of PRT1, PRT2, and PRT4 confirms the threading of MC by the respective axles, which are coordinated to the CuII center with Cu–N bond lengths of 1.992–2.203 Å. Interestingly, strong aromatic π–π stacking interactions between two parallel arene moieties of MC and the pyridyl unit of the axle with π–π interaction distances of 3.402–3.618 Å are observed in all three [2]pseudorotaxanes. However, for PRT4, a strong hydrogen‐bonding interaction is also operative between the carbonyl oxygen atom of BPy5Bnz and the amide proton of MC with a N–H···O distance of 2.200 Å. Thus, multiple template interactions such as metal coordination, π–π stacking, and second‐sphere hydrogen‐bonding interactions between MC and the axles play crucial roles for facile threading and high‐yield syntheses of [2]pseudorotaxanes.
Influence of carbon and metal oxide nanomaterials on aqueous concentrations of the munition constituents cyclotrimethylenetrinitramine (RDX) and tungsten
Authors: Brame Jonathon A. , Kennedy Alan J. , Lounds Christopher D. , Bednar Anthony J. , Alvarez Pedro J.J. , Scott Andrea M. , Stanley Jacob K. 
Published: January 2014

Abstract

There is an increasing likelihood of interactions between nanomaterials and munitions constituents in the environment resulting from the use of nanomaterials as additives to energetic formulations and potential contact in waste streams from production facilities and runoff from training ranges. The purpose of the present research was to determine the ability of nano-aluminum oxide (Al2O3) and multiwalled carbon nanotubes (MWCNTs) to adsorb the munitions constituents cyclotrimethylenetrinitramine (RDX) and tungsten (W) from aqueous solution as a first step in determining the long-term exposure, transport, and bioavailability implications of such interactions. The results indicate significant adsorption of RDX by MWCNTs and of W by nano-Al2O3 (but not between W and MWCNT or RDX and nano-Al2O3). Kinetic sorption and desorption investigations indicated that the most sorption occurs nearly instantaneously (&lt;5 min), with a relatively slower, secondary binding leading to statistically significant but relatively smaller increases in adsorption over 30 d. The RDX sorption that occurred during the initial interaction was irreversible, with long-term, reversible sorption likely the result of a secondary interaction; as interaction time increased, however, the portion of W irreversibly sorbed onto nano-Al2O3 also increased. The present study shows that strong interactions between some munitions constituents and nanomaterials following environmental release are likely. Time-dependent binding has implications for the bioavailability, migration, transport, and fate of munitions constituents in the environment. Environ Toxicol Chem 2014;33:1035–1042. © 2014 SETAC
Ecotoxicological effects of perfluorooctanoic acid on freshwater microalgae Chlamydomonas reinhardtii and Scenedesmus obliquus
Authors: Hu Changwei , Luo Qi , Huang Qingguo 
Published: January 2014

Abstract

As a persistent bioaccumulative compound, perfluorooctanoic acid (PFOA) is found in various ecosystems and receives growing attention. The acute toxicity of PFOA was tested on 2 freshwater microalgae, Chlamydomonas reinhardtii and Scenedesmus obliquus. The 96-h concentration for 50% of maximal effect (EC50) values were measured, physiological responses of the algae were investigated, and uptake of PFOA by the algae was quantified. The EC50 values for C. reinhardtii and S. obliquus were 51.9 ± 1.0 mg/L and 44.0 ± 1.5 mg/L PFOA, respectively. After 8-d exposure to PFOA ranging from 10 mg/L to 40 mg/L, the growth of C. reinhardtii was significantly inhibited, whereas that of S. obliquus was only slightly suppressed. Increases in malonaldehyde and proline levels were observed in the 2 algae when exposed to PFOA at certain concentrations, for instance, 20 mg/L and 40 mg/L, which is indicative of the trigger of a defensive mechanism. The percentage of PFOA that was adsorbed by the algae after 8-d exposure at a dosage between 5 mg/L and 20 mg/L ranged from 5.5% to 7.5%, and the uptake of PFOA by the algae exceeded 10%. Environ Toxicol Chem 2014;33:1129–1134. © 2014 SETAC
Vestibular loss promotes procedural response during a spatial task in rats
Authors: Machado Marie‐Laure , Lelong‐Boulouard Véronique , Philoxene Bruno , Davis Audrey , Denise Pierre , Besnard Stéphane 
Published: January 2014

ABSTRACTDeclarative memory refers to a spatial strategy using numerous sources of sensory input information in which visual and vestibular inputs are assimilated in the hippocampus. In contrast, procedural memory refers to a response strategy based on motor skills and familiar gestures and involves the striatum. Even if vestibular loss impairs hippocampal activity and spatial memory, vestibular‐lesioned rats remain able to find food rewards during complex spatial memory task. Since hippocampal lesions induce a switch from declarative memory to procedural memory, we hypothesize that vestibular‐lesioned rats use a strategy other than that of hippocampal spatial response to complete the task and to counterbalance the loss of vestibular information. We test, in a reverse T‐maze paradigm, the types of strategy vestibular‐lesioned rats preferentially uses in a spatial task. We clearly demonstrate that all vestibular‐lesioned rats shift to a response strategy to solve the spatial task, while control rats use spatial and response strategies equally. We conclude that the loss of vestibular informations leading to spatial learning impairments is not offset at the hippocampus level by integration process of other sense mainly visual informations; but favors a response strategy through procedural memory most likely involving the striatum, cerebellum, and motor learning. © 2014 Wiley Periodicals, Inc.
Sleeping Beauty–baculovirus hybrid vectors for long‐term gene expression in the eye
Authors: Turunen Tytteli Anni Kaarina , Laakkonen Johanna Päivikki , Alasaarela Laura , Airenne Kari Juhani , Ylä‐Herttuala Seppo 
Published: January 2014

AbstractBackgroundA baculovirus vector is capable of efficiently transducing many nondiving and diving cell types. However, the potential of baculovirus is restricted for many gene delivery applications as a result of the transient gene expression that it mediates. The plasmid‐based Sleeping Beauty (SB) transposon system integrates transgenes into target cell genome efficiently with a genomic integration pattern that is generally considered safer than the integration of many other integrating vectors; yet efficient delivery of therapeutic genes into cells of target tissues in vivo is a major challenge for nonviral gene therapy. In the present study, SB was introduced into baculovirus to obtain novel hybrid vectors that would combine the best features of the two vector systems (i.e. effective gene delivery and efficient integration into the genome), thus circumventing the major limitations of these vectors.MethodsWe constructed and optimized SB‐baculovirus hybrid vectors that bear either SB100x transposase or SB transposon in the forward or reverse orientations with respect to the viral backbone The functionality of the novel hybrid vectors was investigated in cell cultures and in a proof‐of‐concept study in the mouse eye.ResultsThe hybrid vectors showed high and sustained transgene expression that remained stable and demonstrated no signs of decline during the 2 months follow‐up in vitro. These results were verified in the mouse eye where persistent transgene expression was detected two months after intravitreal injection.ConclusionsOur results confirm that (i) SB‐baculovirus hybrid vectors mediate long‐term gene expression in vitro and in vivo, and (ii) the hybrid vectors are potential new tools for the treatment of ocular diseases. Copyright © 2014 John Wiley & Sons, Ltd.
Review of hepatopancreatoduodenectomy for biliary cancer: an extended radical approach of Japanese origin
Authors: Ebata Tomoki , Yokoyama Yukihiro , Igami Tsuyoshi , Sugawara Gen , Mizuno Takashi , Nagino Masato 
Published: January 2014

Abstract Cholangiocarcinomas exhibit various modes of local extension, and some tumors can only be completely resected by hepatopancreatoduodenectomy (HPD), which is defined as the resection of the whole extrahepatic biliary system with the adjacent liver and pancreatoduodenum. Since Takasaki et al. introduced HPD for locally advanced gallbladder cancer in 1980, Japanese hepatobiliary surgeons have aggressively challenged this extended procedure for advanced biliary tumors. Early experiences with HPD were frequently associated with liver failure and sequential mortality, leading to an underestimation of the survival benefit of HPD. However, with improvements in surgical techniques and perioperative patient care, including portal vein embolization, over the last two decades, the mortality rate after HPD has gradually decreased. Recent studies have demonstrated a favorable survival in cholangiocarcinoma, provided that R0 resection is achieved. In contrast, HPD for gallbladder cancer remains controversial because of the extremely poor survival, although the study populations have been limited. HPD can be performed with low mortality and offers a better probability of long‐term survival in patients with cholangiocarcinoma. We should consider HPD to be a standard approach for laterally advanced cholangiocarcinomas that are otherwise unresectable.
Recent advances in 3D computed tomography techniques for simulation and navigation in hepatobiliary pancreatic surgery
Authors: Uchida Masafumi 
Published: January 2014

Abstract A few years ago it could take several hours to complete a 3D image using a 3D workstation. Thanks to advances in computer science, obtaining results of interest now requires only a few minutes. Many recent 3D workstations or multimedia computers are equipped with onboard 3D virtual patient modeling software, which enables patient‐specific preoperative assessment and virtual planning, navigation, and tool positioning. Although medical 3D imaging can now be conducted using various modalities, including computed tomography (CT), magnetic resonance imaging (MRI), positron emission tomography (PET), and ultrasonography (US) among others, the highest quality images are obtained using CT data, and CT images are now the most commonly used source of data for 3D simulation and navigation image. If the 2D source image is bad, no amount of 3D image manipulation in software will provide a quality 3D image. In this exhibition, the recent advances in CT imaging technique and 3D visualization of the hepatobiliary and pancreatic abnormalities are featured, including scan and image reconstruction technique, contrast‐enhanced techniques, new application of advanced CT scan techniques, and new virtual reality simulation and navigation imaging.
Synthesis and Characterization of Poly(phenylacetylene)s with Ru(II) Bis‐Terpyridine Complexes in the Side‐Chain
Authors: Breul Alexander M. , Kübel Joachim , Häupler Bernhard , Friebe Christian , Hager Martin D. , Winter Andreas , Dietzek Benjamin , Schubert Ulrich S. 
Published: January 2014

An alkyne‐functionalized ruthenium(II) bis‐terpyridine complex is directly copolymerized with phenylacetylene by alkyne polymerization. The polymer is characterized by size‐exclusion chromatography (SEC), 1H NMR spectroscopy, cyclic voltammetry (CV) measurements, and thermal analysis. The photophysical properties of the polymer are studied by UV–vis absorption spectroscopy. In addition, spectro‐electrochemical measurements are carried out. Time‐resolved luminescence lifetime decay curves show an enhanced lifetime of the metal complex attached to the conjugated polymer backbone compared with the Ru(tpy)22+ model complex.
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