bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pemanfaatan Kotoran Hewan dan Limbah Pertanian sebagai Kompos dalam Usaha Peningkatan Produksi Sayuran di Desa Ake Ara Jailolo Selatan Halmahera Barat
Authors: Sofyan Adnan , Rachman Idris Abd , Hartono Gunawan , Hasan Asrul Dedy Ali 
Published: July 2024

Abstrak
Pemanfaatan limbah pertanian dan kotoran hewan sebagai kompos belum maksimal dilakukan oleh masyarakat tani dalam mengatasi kelangkaan pupuk di masyarakat tani. Pembuatan kompos merupakan alternatif dalam menciptakan pupuk alami yang dibuat oleh petani sendiri sehingga menjawab kelangkaan pupuk yang pada gilirannya meningkatkan kualitas kesehatan tanah, menghasilkan produk tanaman yang sehat, dan meningkatkan pendapatan petani. Solusi yang ditawarkan dalam program yaitu penyajian materi pembuatan kompos, cara aplikasi ke tanaman, pendampingan dalam kegiatan usaha budidaya tanaman, penangan pascapanen sehingga produk tanaman bisa terjaga secara sehat. Diharapkan petani sebagai mitra yang didampingi dapat menghasilkan pupuk organik kompos tersebut dapat meningkatkan produksi tanaman dan peningkatan pendapatannya. Tujuan program ini untuk meningkat ketrampilan petani dalam memanfaatkan serasah tanaman, limbah pertanian, kotoran hewan untuk dijadikan pupuk kompos, yang nantinya pupuk kompos tersebut dapat dijual dipasaran maupun digunakan petani sendiri dalam peningkatan usaha budidaya tanaman sayuran. Tahapan kegiatan dari sosialisasi, penyajian materi penyuluhan, pembuatan demonstrasi plot (demplot) pupuk kompos dan demplot budidaya tanaman dengan tahapan penyiapan lahan, persemaian, pembuatan petakan, penanaman, pemupukan, pengairan, pemeliharaan tanaman sampai panen dan pemasaran hasil budidaya tanaman yang diusahakan.
Optimalisasi Pemberdayaan Masyarakat Melalui Pembibitan Tanaman Obat (Biofarmaka)
Authors: Salatalohy Asiah , Nurhikmah  , Marasabessy Muh. Hidayah , Tidore Muh Faedly 
Published: July 2024

Upaya masyarakat dalam mengobati penyakit di Dusun Bangko Desa Bobaneigo Halmahera Barat lebih banyak dilakukan melalui pengobatan tradisional dengan memanfaatkan tumbuhan obat. Namun kebanyakan mereka belum melakukan pembudidayaan tanaman obat melalui pembibitan. Hal ini disebabkan kurangnya informasi, motivasi dan pengetahuan masyarakat tentang manfaat dan pembibitan tumbuhan obat tersebut. Jenis tumbuhan obat yang ditanam warga di Dusun Bangko Desa Bobaneigo pun dalam kuantitas sangat kurang memadai di sekitar rumah sekaligus tanaman hias seperti daun miyana, kembang sepatu dan kumis kucing. Kegiatan pembibitan untuk jenis lainnya belum dilakukan. Kegiatan pengabdian masyarakat ini secara umum bertujuan untuk membantu warga masyarakat dalam membuat pembibitan tanaman obat (biofarmaka). Hal ini merupakan upaya untuk meningkatkan pendapatan keluarga. Secara khusus kegiatan ini bertujuan agar setelah selesai mengikuti kegiatan, peserta akan mampu memahami dan termotivasi dalam membuat pembibitan tanaman obat, memiliki pengetahuan dalam pembuatan pembibitan serta mengolah dan memasarkan tanaman obat sebagai upaya untuk meningkatkan pendapatan keluarga. Dalam pembibitan tanaman obat jenis yang dikembangkan adalah jahe merah (Zingiber officinale), kunyit (Curcuma longa Linn), dan sereh (Cymbopogon citratus). Tanaman tersebut sangat menjanjikan di saat situasi Pandemi covid tahun 2020 lalu. Metode yang digunakan dalam kegiatan ini yaitu dengan membuat demplot langsung di lahan sekitar kampus 4 Universitas Khairun dengan pendekatan Pendidikan Orang Dewasa (POD), ceramah, diskusi dan bimbingan. Dengan adanya usaha pengelolaan ini diharapkan akan turut meningkatkan pengetahuan dan pendapatan masyarakat.
Kata Kunci: biofarmaka, optimalisasi, pemberdayaan, tanaman obat
LITERATURE REVIEW OF THE USE OF YOUTUBE TO MOTIVATE STUDENTS IN LEARNING ENGLISH
Authors: Putri Alyssa Keysha , Nugroho Inayah Putri , Anditirta Aldriani , Nuraini Hery 
Published: July 2024

YouTube is an online media platform containing a variety of videos. It is also a place to post, share, and download videos.Its users range from children, teenagers, adults to parents, and no exception including students. This study aims to investigate the effect of using YouTube on students’ motivation in learning English. A qualitative method was used in this study, its study literature review. This research used descriptive research design to process the data and to draw the conclusions. Researcher gathered data from collecting literature to describe the situation related to the use of Youtube to motivate students in learning English. The result of this study was found out the implementation of YouTube videos in learning English was showed a positive impact on students to be more motivated.
EEKSISTENSI TRADISI MELEPAS AYAM PADA PERKAWINAN ADAT JAWA : PERSPEKTIF HUKUM ISLAM
Authors: Nafs Laila Shafa'un , Afif Abdullah 
Published: July 2024

Salah satu upacara perkawinan adat jawa yang masih kita lihat di desa Bedahlawak Kecamatan Tembelang Kabupaten Jombang yakni tradisi melepas ayam pada perkawinan jawa. Penelitian ini bertujuan untuk memahami pelaksanaan tradisi melepas ayam dalam perkawinan adat Jawa di desa Bedahlawak, serta pandangan masyarakat muslim terhadap tradisi tersebut dan kaitannya dengan hukum islam. Melalui penelitian kualitatif lapangan yang bersifat empiris dengan pendekatan sosiologi hukum, dengan menggunakan teknik pengumpulan data primer dari lapangan berupa observasi, wawancara dan dakumentasi serta data sekunder.Proses pelaksanaan tradisi ini dilakukan pada saat iring iring pengantin yang mana rumah mempelai pengantin dipisahkan dengan sungai besar. pelaksanaan tradisi ini cukup singkat dimulai dari pembacaan doa dan sebelum menyeberangi jembatan sungai besar ayam akan dilepaskan di tepi jembatan. Terdapat masyarakat yang melakukan dan meninggalkan tradisi ini. Kemudian ditemukan variasi. Jika tradisi dilakukan dengan niat meminta pertolongan kepada selain Allah SWT, dapat dianggap sebagai praktik yang haram dilakukan dan termasuk urf’ fasid, namun jika tujuannya baik dan hanya memohon keselamatan kepada Allah SWT tanpa tujuan yang menimpang, mak termasuk dalam praktik yang sesuai dengan hukum islam dan termasuk urf’ shahih.
Endophthalmitis: a bibliometric study and visualization analysis from 1993 to 2023
Authors: Fu Xiangyu , Du Wenyu , Huang Ling , Ren Xiang , Chen Danian 
Published: July 2024

AimsThis study is designed to generalize and depict the research hotspots of endophthalmitis through bibliometric methods and software and analyze the evolutive tendency of the work on this severe disease over the past 30 years.MethodsThis study employed a rigorous bibliometric approach. We identified all endophthalmitis-related literature by conducting a comprehensive search of the Science Citation Index Expanded database under the Web of Science Core Collection. The data was then analyzed and visualized using CiteSpace and VOSviewer, two widely recognized software tools in the field of bibliometrics. CiteSpace was used to analyze the country distributions, dual map overlay of journals, keyword bursts, and co-cited references. VOSviewer was employed to describe the authors and co-cited authors, the journals, the co-cited journals, and the keywords co-occurrence network. This robust methodology ensures the reliability and validity of the study’s findings.ResultsA total of 2960 publications, including 2695 articles and 265 reviews, were included in this bibliometric study. There has been no shortage of endophthalmitis-related publications since 1993, with an apparent upward trend during recent years. Possible correlations with the COVID-19 pandemic are also analyzed. These studies were finished by 11,048 authors from 75 countries worldwide, with the United States in the lead. In the keyword co-occurrence network, except for the endophthalmitis term, cataract surgery becomes the keyword with the highest frequency. Different categories of endophthalmitis, including postoperative, post-injection, post-traumatic, and endogenous endophthalmitis, and antibacterial and anti-inflammatory therapies of infectious endophthalmitis, are discussed by categories. From the perspective of the timeline, postoperative and post-injection endophthalmitis were the dominant forms before and after the year 2000, respectively. Co-citation analyses reveal that the Endophthalmitis Vitrectomy Study (EVS) conducted in 1995 provides pivotal guidance for later research. Diverse pathogenic bacteria (e.g., Coagulase-negative Staphylococci, Propionibacterium acnes, Viridians Streptococci, and Bacillus cereus) or fungi (e.g., Candida, Aspergillus, and Fusarium) contribute to varying treatment principles and clinical prognosis, which should be taken seriously. In addition, intravitreal and intracameral antibiotics are the mainstay for treating and preventing infectious endophthalmitis, respectively.ConclusionOur bibliometric analysis provides an overview of dynamic evolution and structural relationships in the research field of endophthalmitis. The displayed hotspots and developmental directions have reference values for future investigation.
Analysis of Amblyomma americanum microRNAs in response to Ehrlichia chaffeensis infection and their potential role in vectorial capacity
Authors: Kumar Deepak , Budachetri Khemraj , Rikihisa Yasuko , Karim Shahid 
Published: July 2024

BackgroundMicroRNAs (miRNAs) represent a subset of small noncoding RNAs and carry tremendous potential for regulating gene expression at the post-transcriptional level. They play pivotal roles in distinct cellular mechanisms including inhibition of bacterial, parasitic, and viral infections via immune response pathways. Intriguingly, pathogens have developed strategies to manipulate the host’s miRNA profile, fostering environments conducive to successful infection. Therefore, changes in an arthropod host’s miRNA profile in response to pathogen invasion could be critical in understanding host-pathogen dynamics. Additionally, this area of study could provide insights into discovering new targets for disease control and prevention. The main objective of the present study is to investigate the functional role of differentially expressed miRNAs upon Ehrlichia chaffeensis, a tick-borne pathogen, infection in tick vector, Amblyomma americanum.MethodsSmall RNA libraries from uninfected and E. chaffeensis-infected Am. americanum midgut and salivary gland tissues were prepared using the Illumina Truseq kit. Small RNA sequencing data was analyzed using miRDeep2 and sRNAtoolbox to identify novel and known miRNAs. The differentially expressed miRNAs were validated using a quantitative PCR assay. Furthermore, a miRNA inhibitor approach was used to determine the functional role of selected miRNA candidates.ResultsThe sequencing of small RNA libraries generated &gt;147 million raw reads in all four libraries and identified a total of &gt;250 miRNAs across the four libraries. We identified 23 and 14 differentially expressed miRNAs in salivary glands, and midgut tissues infected with E. chaffeensis, respectively. Three differentially expressed miRNAs (miR-87, miR-750, and miR-275) were further characterized to determine their roles in pathogen infection. Inhibition of target miRNAs significantly decreased the E. chaffeensis load in tick tissues, which warrants more in-depth mechanistic studies.ConclusionsThe current study identified known and novel miRNAs and suggests that interfering with these miRNAs may impact the vectorial capacity of ticks to harbor Ehrlichia. This study identified several new miRNAs for future analysis of their functions in tick biology and tick-pathogen interaction studies.
Persistence, changes and robustness of nest webs along a latitudinal gradient in the Canadian boreal forest
Authors: Cadieux Philippe , Drapeau Pierre , Fouillet Alexandre , Deschênes Réjean 
Published: July 2024

IntroductionIn eastern Canada, the boreal forest is associated with an important latitudinal shift in forest composition and structure, which occurs in the transition between the mixed southern boreal forest and the coniferous northern boreal forest. Along this transition, upland mixedwood stands with large deciduous trees (important for cavity-dependent vertebrate species) are gradually replaced by forests with smaller conifer trees, primarily black spruce (Picea mariana). Concomitantly, the availability of lowland forests flooded by the American beaver (Castor canadensis), which can provide adequate conditions for tree-cavity users, is also decreasing.MethodsWe hypothesized that this latitudinal gradient would bring important changes in the functional diversity and network structure of vertebrate cavity-using communities. Along this latitudinal gradient we used a nest web approach to analyze the structure and robustness of networks of cavity users in upland forests and in lowland forests flooded by beavers.ResultsDespite their low availability in the northern forest region, we found that mixedwood stands persisted throughout the boreal forest in being the main drivers of nest webs network structure of upland forests whereas old black spruce stands contribution was low. In lowland forests, beaver ponds harbored nest webs with a rich and complex structure in both forest regions. Species removal simulations revealed that across our latitudinal gradient upland and lowland forest nest webs responded differently. In upland forests, the removal of trembling aspen and the Pileated Woodpecker (Dryocopus pileatus) caused the highest proportions of secondary extinctions, showing low robustness of nest webs given that these two species were highly connected to the other species. Contrastingly, nest webs in beaver ponds were more robust mainly because excavator species used a higher diversity of tree species despite the removal of the Northern Flicker (Colaptes auratus) which induced numerous secondary extinctions. The Pileated Woodpecker remained the pivotal species across the two forest regions in upland forests whereas the Northern Flicker became the main large cavity provider in beaver ponds across the latitudinal gradient.DiscussionWe discuss how mixedwood forests and beaver ponds, which are key habitat types for the cavity-using vertebrate community across our latitudinal gradient, should be maintained and protected in landscapes under industrial timber harvesting.
Characterization of latently infected EBV+ antibody-secreting B cells isolated from ovarian tumors and malignant ascites
Authors: Zhang Lixin , Strange Mary , Elishaev Esther , Zaidi Syed , Modugno Francesmary , Radolec Mackenzy , Edwards Robert P. , Finn Olivera J. , Vlad Anda M. 
Published: July 2024

IntroductionIntra-tumoral B cells mediate a plethora of immune effector mechanisms with key roles in anti-tumor immunity and serve as positive prognostic indicators in a variety of solid tumor types, including epithelial ovarian cancer (EOC). Several aspects of intra-tumoral B cells remain unclear, such as their state of activation, antigenic repertoires, and capacity to mature into plasma cells.MethodsB lymphocytes were isolated from primary EOC tissue and malignant ascites and were maintained in cell culture medium. The stably maintained cell lines were profiled with flow cytometry and B cell receptor sequencing. Secreted antibodies were tested with a human proteome array comprising more than 21,000 proteins, followed by ELISA for validation. Originating tumor samples were used for spatial profiling with chip cytometry.ResultsAntibody-secreting B lymphocytes were isolated from the ovarian tumor microenvironment (TME) of four different EOC patients. The highly clonal cell populations underwent spontaneous immortalization in vitro, were stably maintained in an antibody-secreting state, and showed presence of Epstein-Barr viral (EBV) proteins. All originating tumors had high frequency of tumor-infiltrating B cells, present as lymphoid aggregates, or tertiary lymphoid structures. The antigens recognized by three of the four cell lines are coil-coil domain containing protein 155 (CCDC155), growth factor receptor-bound protein 2 (GRB2), and pyruvate dehydrogenase phosphatase2 (PDP2), respectively. Anti-CCDC155 circulating IgG antibodies were detected in 9 of 20 (45%) of EOC patients’ sera. Tissue analyses with multiparameter chip cytometry shows that the antibodies secreted by these novel human B cell lines engage their cognate antigens on tumor cells.DiscussionThese studies demonstrate that within the tumor-infiltrating lymphocyte population in EOC resides a low frequency population of antibody-secreting B cells that have been naturally exposed to EBV. Once stably maintained, these novel cell lines offer unique opportunities for future studies on intratumor B cell biology and new target antigen recognition, and for studies on EBV latency and/or viral reactivation in the TME of non-EBV related solid tumors such as the EOC.
Circulating immune cells and risk of osteosarcoma: a Mendelian randomization analysis
Authors: Li Lan , Sun Yeqi , Luo Jia , Liu Mengjiao 
Published: July 2024

ObjectivesOsteosarcoma (OS) is the primary bone tumor originating from transformed mesenchymal cells. It is unclear whether associations between specific circulating immune cells and OS are causal or due to bias. To clarify whether predicted genetically altered circulating immune cells are associated with OS development, we performed a two-sample Mendelian randomization (MR) analysis.MethodsThe genetic variants strongly associated with immune cell traits as instrumental variables (IVs) were used to perform MR analyses. The effect of specific immune cells on OS risk was measured using the summary statistics from the genome-wide association studies (GWAS).ResultsOur findings indicate that CD80 on CD62L+ myeloid dendritic cell and CD28−CD4−CD8− T-cell absolute count are positively associated with OS (CD80 on CD62L+ myeloid dendritic cell, OR: 3.41 [95% CI: 1.40 to 8.31], p = 0.007; CD28−CD4−CD8− T-cell absolute count, OR: 4.49 [95% CI: 1.29 to 15.62], p = 0.018). It is also found that CD20 has a negative effect on CD24+CD27+ B cell on OS (OR: 0.32 [95% CI: 0.14 to 0.72], p = 0.006) and a similar impact on IgD+ CD38− B cell on OS (OR: 0.19 [95% CI: 0.05 to 0.68], p = 0.011).ConclusionsThese findings illustrate that the genetic predisposition to specific immune cells can exert a causal effect on OS risk, which confirms the crucial role played by immunity in OS development. Particularly, the causal association between immune cells and OS underscores the evidence for exploring the new treatment strategy for OS in the future.
Protective role of the CD73-A2AR axis in cirrhotic cardiomyopathy through negative feedback regulation of the NF-κB pathway
Authors: Zhao Ning , Shao Zhenhao , Xia Guoqing , Liu Huanhuan , Zhang Lei , Zhao Xiaoxi , Dang Shipeng , Qian Lingling , Xu Wentao , Yu Zhiming , Wang Ruxing 
Published: July 2024

BackgroundMyocardial inflammation and apoptosis induced by cirrhosis are among the primary mechanisms of cirrhotic cardiomyopathy. CD73, a common extracellular nucleotidase also known as 5’-nucleotidase, is associated with the progression of inflammation and immunity in multiple organs. However, the mechanism by which CD73 contributes to myocardial inflammation and apoptosis in cirrhosis remains unclear.MethodsIn this study, a cirrhotic cardiomyopathy model in mice was established by bile duct ligation. Myocardial-specific overexpression of CD73 was achieved by tail vein injection of AAV9 (adeno-associated virus)-cTNT-NT5E-mCherry, and cardiac function in mice was assessed using echocardiography. Myocardial inflammation infiltration and apoptosis were evaluated through pathological observation and ELISA assays. The expression of CD73, A2AR, apoptotic markers, and proteins related to the NF-κB pathway in myocardial tissue were measured.ResultsIn the myocardial tissue of the cirrhotic cardiomyopathy mouse model, the expression of CD73 and A2AR increased. Overexpression of CD73 in the myocardium via AAV9 injection and stimulation of A2AR with CGS 21680 inhibited myocardial inflammation and cardiomyocyte apoptosis induced by cirrhosis. Additionally, overexpression of CD73 suppressed the activation of the NF-κB pathway by upregulating the expression of the adenosine receptor A2A.ConclusionOur study reveals that the CD73/A2AR signaling axis mitigates myocardial inflammation and apoptosis induced by cirrhosis through negative feedback regulation of the NF-κB pathway.
Elucidating the multifaceted role of MGAT1 in hepatocellular carcinoma: integrative single-cell and spatial transcriptomics reveal novel therapeutic insights
Authors: Li Yang , Chen Yuan , Wang Danqiong , Wu Ling , Li Tao , An Na , Yang Haikun 
Published: July 2024

BackgroundGlycosyltransferase-associated genes play a crucial role in hepatocellular carcinoma (HCC) pathogenesis. This study investigates their impact on the tumor microenvironment and molecular mechanisms, offering insights into innovative immunotherapeutic strategies for HCC.MethodsWe utilized cutting-edge single-cell and spatial transcriptomics to examine HCC heterogeneity. Four single-cell scoring techniques were employed to evaluate glycosyltransferase genes. Spatial transcriptomic findings were validated, and bulk RNA-seq analysis was conducted to identify prognostic glycosyltransferase-related genes and potential immunotherapeutic targets. MGAT1’s role was further explored through various functional assays.ResultsOur analysis revealed diverse cell subpopulations in HCC with distinct glycosyltransferase gene activities, particularly in macrophages. Key glycosyltransferase genes specific to macrophages were identified. Temporal analysis illustrated macrophage evolution during tumor progression, while spatial transcriptomics highlighted reduced expression of these genes in core tumor macrophages. Integrating scRNA-seq, bulk RNA-seq, and spatial transcriptomics, MGAT1 emerged as a promising therapeutic target, showing significant potential in HCC immunotherapy.ConclusionThis comprehensive study delves into glycosyltransferase-associated genes in HCC, elucidating their critical roles in cellular dynamics and immune cell interactions. Our findings open new avenues for immunotherapeutic interventions and personalized HCC management, pushing the boundaries of HCC immunotherapy.