bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the role of a Tannerella forsythia HtrA protease in host protein degradation and inflammatory response
Authors: Bloch Susanne , Hager-Mair Fiona F. , Bacher Johanna , Tomek Markus B. , Janesch Bettina , Andrukhov Oleh , Schäffer Christina 
Published: July 2024

IntroductionDegradation of host proteins by bacterial proteases leads to the subversion of the host response and disruption of oral epithelial integrity, which is considered an essential factor in the progression of periodontitis. High-temperature requirement A (HtrA) protease, which is critical for bacterial survival and environmental adaptation, is found in several oral bacteria, including the periodontal pathogen Tannerella forsythia. This study investigated the proteolytic activity of HtrA from T. forsythia and its ability to modulate the host response.MethodsHtrA of T. forsythia was identified bioinformatically and produced as a recombinant protein. T. forsythia mutants with depleted and restored HtrA production were constructed. The effect of T. forsythia wild-type, mutants and recombinant HtrA on the degradation of casein and E-cadherin was tested in vitro. Additionally, the responses of human gingival fibroblasts and U937 macrophages to the different HtrA-stimuli were investigated and compared to those triggered by the HtrA-deficient mutant.ResultsT. forsythia wild-type producing HtrA as well as the recombinant enzyme exhibited proteolytic activity towards casein and E-cadherin. No cytotoxic effect of either the wild-type, T. forsythia mutants or rHtrA on the viability of host cells was found. In hGFB and U937 macrophages, both T. forsythia species induced an inflammatory response of similar magnitude, as indicated by gene and protein expression of interleukin (IL)-1β, IL-6, IL-8, tumour necrosis factor α and monocyte chemoattractant protein (MCP)-1. Recombinant HtrA had no significant effect on the inflammatory response in hGFBs, whereas in U937 macrophages, it induced a transient inflammatory response at the early stage of infection.ConclusionHtrA of T. forsythia exhibit proteolytic activity towards the host adhesion molecule E-cadherin and has the potential to influence the host response. Its role in the progression of periodontitis needs further clarification.
The roles of phosphorylation of signaling proteins in the prognosis of acute myeloid leukemia
Authors: Márton Adrienn , Veres Katalin Beáta , Erdődi Ferenc , Udvardy Miklós , Illés Árpád , Rejtő László 
Published: July 2024

Signaling pathways of Retinoblastoma (Rb) protein, Akt-kinase, and Erk-kinase (extracellular signal-regulated kinase) have an important role in the pathogenesis of acute myeloid leukemia. Constitutive activation of these proteins by phosphorylation contributes to cell survival by regulation of cell cycle, proliferation and proapoptotic signaling processes. According to previous data phosphorylated forms of these proteins represent a worse outcome for cancer patients. We investigated the presence of phosphorylated Rb (P-Rb), Akt (P-Akt) and Erk (P-Erk) proteins by Western blot technique using phospho-specific antibodies in bone marrow or peripheral blood samples of 69 AML patients, 36 patients with myelodysplastic syndrome (MDS) and 10 healthy volunteers. Expression level of PTEN (Phosphatase and tensin homolog) and PHLPP (PH domain and leucine-rich repeat Protein Phosphatase) phosphatases, the negative regulators of Akt kinase pathway were also examined. We tested the effect of these proteins on survival and on the correlation with known prognostic features in AML. We found 46.3% of AML patients had detectable P-Rb, 34.7% had P-Akt and 28.9% had P-Erk protein. 66.1% of patients expressing PTEN, 38.9% PHLPP, 37.2% both PTEN and PHLPP and 32.2% neither PTEN nor PHLPP phosphatases. Compared to nucleophosmin mutation (NPMc) negative samples P-Erk was significantly less in nucleophosmin mutated patients, P-Rb was significantly less in patients’ group with more than 30 G/L peripheral leukocyte count by diagnosis. PHLPP was significantly present in FAB type M5. The expression of P-Rb represented significant better overall survival (OS), while P-Akt represented significantly worse event-free survival (EFS) in unfavorable cytogenetics patients. The presence of both PHLPP and PTEN phosphatases contributes to better OS and EFS, although the differences were not statistically significant. We confirmed significant positive correlation between P-Akt and PHLPP. Assessing the phosphorylation of Rb, Akt and Erk may define a subgroup of AML patients who would benefit especially from new targeted treatment options complemented the standard chemotherapy, and it may contribute to monitoring remission, relapse or progression of AML.
An Improved Instance Segmentation Method for Fast Assessment of Damaged Buildings Based on Post-Earthquake UAV Images
Authors: Zou Ran , Liu Jun , Pan Haiyan , Tang Delong , Zhou Ruyan 
Published: July 2024

Quickly and accurately assessing the damage level of buildings is a challenging task for post-disaster emergency response. Most of the existing research mainly adopts semantic segmentation and object detection methods, which have yielded good results. However, for high-resolution Unmanned Aerial Vehicle (UAV) imagery, these methods may result in the problem of various damage categories within a building and fail to accurately extract building edges, thus hindering post-disaster rescue and fine-grained assessment. To address this issue, we proposed an improved instance segmentation model that enhances classification accuracy by incorporating a Mixed Local Channel Attention (MLCA) mechanism in the backbone and improving small object segmentation accuracy by refining the Neck part. The method was tested on the Yangbi earthquake UVA images. The experimental results indicated that the modified model outperformed the original model by 1.07% and 1.11% in the two mean Average Precision (mAP) evaluation metrics, mAPbbox50 and mAPseg50, respectively. Importantly, the classification accuracy of the intact category was improved by 2.73% and 2.73%, respectively, while the collapse category saw an improvement of 2.58% and 2.14%. In addition, the proposed method was also compared with state-of-the-art instance segmentation models, e.g., Mask-R-CNN and YOLO V9-Seg. The results demonstrated that the proposed model exhibits advantages in both accuracy and efficiency. Specifically, the efficiency of the proposed model is three times faster than other models with similar accuracy. The proposed method can provide a valuable solution for fine-grained building damage evaluation.
Brain Chlamydiosis in Patients with Schizophrenia (Lethal Cases from Practice)
Authors: Цинзерлинг В.А. , Луговская Н.А. , Семенова Н.Ю. , Вайншенкер Ю.И. , Орлова В.А. , Михайлова И.И. 
Published: July 2024

В работе описаны 2 клинических случая смерти: 37-летней женщины, на протяжении 27 лет страдавшей параноидной формой шизофрении и погибшей на фоне осложнений новой коронавирусной инфекции, и 47-летнего мужчины, длительное время страдавшего простой шизофренией с непрерывным течением и умершего в связи с осложнениями перфоративного калькулезного холецистита. При патологоанатомическом исследовании на основании гистологического и иммуногистохимического исследований у обоих пациентов диагностирована хламидийная инфекция (в первом случае генерализованная) с массивным поражением белого и серого вещества головного мозга. Обсуждается вопрос о возможной связи в данных наблюдениях шизофрении с хламидийной инфекцией.
The paper describes two clinical cases of death: a 37-year-old woman who suffered from a paranoid form of schizophrenia for 27 years and died due to complications of a new coronavirus infection, and a 47-year-old man who suffered from simple schizophrenia with a continuous course for a long time and died due to complications of perforated calculous cholecystitis. During the pathological examination, based on histological and immunohistochemical studies, both patients were diagnosed with chlamydia infection (generalized in the first case), with massive damage to the white and gray matter of the brain. The question of a possible connection in these observations of schizophrenia with chlamydia infection is discussed.
On the necessity of using QGIS geoinformation system for forest fire forecasting
Authors: Молодец С.Н. 
Published: July 2024

Лесные пожары представляют собой серьезную экологическую и экономическую угрозу по всему миру. Они не только уничтожают лесные массивы, но и оказывают негативное воздействие на биоразнообразие, изменяют экосистемы, ухудшают качество воздуха и воды, а также наносят значительный ущерб имуществу и инфраструктуре. В последние годы в связи с изменением климата и антропогенными факторами частота и интенсивность пожаров увеличиваются, что делает проблему еще более актуальной. В таких условиях возникает острая необходимость в разработке и внедрении эффективных методов прогнозирования и управления пожарами. Статья направлена на демонстрацию практической ценности QGIS в контексте мониторинга и предотвращения лесных и ландшафтных пожаров, а также на выявление перспектив дальнейшего использования этой системы в экологических исследованиях.
Forest fires are a serious environmental and economic threat worldwide. They not only destroy forests, but also have negative impacts on biodiversity, alter ecosystems, degrade air and water quality, and cause significant damage to property and infrastructure. In recent years, due to climate change and anthropogenic factors, the frequency and intensity of fires have increased, making the problem even more urgent. Under such conditions, there is an urgent need to develop and implement effective methods of fire prediction and management. The article aims to demonstrate the practical value of QGIS in the context of forest and landscape fire monitoring and prevention, as well as to identify the prospects for further use of this system in ecological research
Microcentrales hidroeléctricas con tecnología turbulent hydro para la generación de electricidad en comunidades aisladas
Authors: Cuichan-Paucar Santiago Homero , Vera-Santi Lizbeth Estefania , Heras-Heras Mayra Carolina , Quevedo-Amay Deisy Valeria 
Published: July 2024

El objetivo de la investigación fue realizar un análisis de microcentrales hidroeléctricas basadas en tecnología hidroeléctrica turbulenta como fuente de energía renovable. El diseño de investigación fue no experimental y nivel descriptivo. Los resultados reflejaron que la tecnología Turbulent Hydro ofrece una eficiencia del 70% al 90% de potencia al convertir la energía del agua en electricidad, produciendo un rango 5 a 70 kW en potencia con una sola turbina, lo que puede llegar a proveer de electricidad a un aproximado de 200 casas, generando una capacidad mayor del 50% en comparación con otras fuentes de energía, las cuales además de ocupar mayor espacio tienen más costos de instalación y mantenimiento. En conclusión, El costo de inversión de una micro central hidroeléctrica con tecnología Turbulent Hydro es de 750 euros o 834,64 dólares, teniendo en cuenta el rango estimado de funcionamiento de entre 15 a 30 años.
Uji Aktivitas Antioksidan Ekstrak Daun Amaranthus hybridus L. dalam Pelarut Heksana, Etil Asetat, dan Air
Authors: Halim Johni , Ranggaini Monica D. , Tedjokusumo Sabrina N. 
Published: July 2024

Abstract: Noncommunicable diseases, such diabetes mellitus, atherosclerosis and cancer, caused by inter alia imbalance between free radicals and antioxidants within the body, known as oxidative stress. Antioxidants from outside the body are required as antioxidants within the body are not enough to ward off excessive free radicals. Amaranthus hybridus L. plant is quite popular among Indonesian people due to its fast harvest and advantages for health, including its natural antioxidants potency. This study aimed to determine the comparison of antioxidant activities among n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. leaves. This was an experimental laboratory study. Amaranthus hybridus L. simplicia extracted with maceration in n-hexane, ethyl acetate and aquadest. Antioxidant activities of n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. were examined with 2.2-diphenyl-1-picrylhydrazyl (DPPH) in 517 nm wavelength by using microplate reader. The results showed that the IC50 values of A. hybridus L. n-hexane, ethyl acetate, and aqueous extract respectively were 131.81 mg/mL, 103.53 mg/mL, and 79.61 mg/mL. In conclusion, there are differences in antioxidant activities between n-hexane, ethyl acetate, and aqueous extract of A. hybridus L. This occur due to differences in the polarity of each solvent.
Keywords: Amaranthus hybridus L.; antioxidant activities; maceration; solvent polarity

Abstrak: Penyakit tidak menular, seperti diabetes melitus, aterosklerosis, dan kanker, disebabkan antara lain oleh ketidakseimbangan antara radikal bebas dan antioksidan dalam tubuh (stres oksidatif). Antioksidan dari luar tubuh diperlukan karena antioksidan dari dalam tubuh tidak cukup untuk menangkal radikal bebas berlebih. Tanaman Amaranthus hybridus L. cukup diminati masyarakat Indonesia karena waktu panen yang singkat dan manfaatnya bagi kesehatan, termasuk potensi antioksidan alami. Penelitian ini bertujuan untuk mengetahui perbedaan aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. Jenis penelitian ini berupa studi eksperimental laboratorik. Simplisia A. hybridus L. diekstraksi dengan metode maserasi dalam pelarut heksana, etil asetat, dan air. Aktivitas antioksidan ekstrak heksana, etil asetat, dan air daun A. hybridus L. diuji aktivitas antioksidan dengan metode 2,2-difenil-1-pikrilhidrazil (DPPH) pada panjang gelombang 517 nm dengan menggunakan microplate reader. Hasil penelitian menunjukkan nilai IC50 ekstrak heksana, etil asetat, dan air daun A. hybridus L. secara berturut-turut sebesar 131,81 mg/mL, 103,53 mg/mL, dan 79,61 mg/mL. Simpulan penelitian ini ialah terdapat perbedaan aktivitas antioksidan antara ekstrak heksana, etil asetat, dan air daun Amaranthus hybridus L. Hal ini terjadi karena perbedaan polaritas dari masing-masing pelarut.
Kata kunci: Amaranthus hybridus L.; aktivitas antioksidan; maserasi; polaritas pelarut
Wafer and chip-level characterization of edge-coupled photonic integrated circuits by cascaded grating couplers and spot-size converters
Authors: Eissa Moataz , Sasaki Ryuya , Horikawa Tsuyoshi , Amemiya Tomohiro , Nishiyama Nobuhiko 
Published: July 2024

Abstract
This study presents an efficient testing process for characterizing silicon photonic ICs. This process utilizes a coupling structure that integrates grating couplers and spot-size converters for efficient testing both at the chip and wafer levels, respectively. By leveraging wafer-level testing to estimate the characteristics of final chip-level devices, we anticipate a reduction in testing costs. To demonstrate the validity of the proposed testing process, we fabricated and measured silicon-on-insulator ring resonator devices on both wafer and chip levels. The results showed good agreement between the two levels of measurement, validating the effectiveness of our proposed testing process.
Inisiasi Produksi Minuman Probiotik untuk Meningkatkan Imunitas Masyarakat di Desa Cinangka, Banten
Authors: Heriatmo Nadia Larasinta , Marwati Umi , Faradiba Faradiba , Laksmitawati Dian Ratih 
Published: July 2024

Sebagai upaya peningkatan daya tahan tubuh dan imunitas masyaratakat adalah dengan meningkatkan kesehatan saluran cerna yang salah satu caranya dengan mengkonsumsi minuman kesehatan yaitu minuman probiotik. Pembuatan minuman ini dapat dilakukan dengan cara sederhana dan menggunakan perlengkapan skala rumah tangga yang dapat dipraktekkan pada lingkungan masyarakat. Sebagai usaha dalam meningkatkan potensi yang dikembangkan pada lingkup kesehatan dan ekonomi maka dilakukan inisiasi ekonomi kreatif melalui penyuluhan dan pelatihan produksi minuman probiotik kepada anggota kader posyandu dari beberapa desa di Kecamatan Cinangka, Banten. Metode kegiatan ini berupa edukasi materi dan praktek langsung. Pelaksanaan dimulai dengan evaluasi awal (pretest) untuk menilai pengetahuan umum dari masyarakat, pemaparan edukasi materi yang diberikan dengan cara presentasi dan dilanjutkan praktek secara langsung cara pembuatan minuman probiotik. Hasil menunjukkan edukasi dapat meningkatkan pemahaman responden mengenai masalah kesehatan dan produk minuman probiotik. Hasil analisa menunjukkan bahwa kegiatan pengabdian kepada masyarakat dapat meningkatkan pemahaman responden mengenai minuman probiotik sebanyak 29%. Seluruh responden berhasil membuat minuman probiotik menggunakan alat dan bahan yang diberikan langsung pada kegiatan ini. Kegiatan ini berpotensi dapat meningkatkan kesehatan dan pergerakan ekonomi masyarakat sekitar. Keberlanjutan dari kegiatan ini direkomendasikan mendapat dukungan dari berbagai pihak agar senantiasa berperan dalam pergerakan ekonomi mandiri sehingga bisa tercapainya standarisasi produk dan pendampingan berkelanjutan oleh pada akademisi dan pemerintahan khususnya di wilayah Kecamatan Cinangka, Banten.
PENGARUH LINGKUNGAN KERJA, PENDELEGASIAN WEWENANG DAN BONUS TERHADAP KEPUASAN KERJA PEGAWAI BPJS KETENAGAKERJAAN CABANG BATAM
Authors: Hakim Lukman , Mustaqim Mustaqim , Suyatni Suyatni , Nurhadiyanti Nurhadiyanti 
Published: July 2024

Penelitian ini bertujuan untuk memperoleh informasi mengenai keterkaitan antara variabel bebas yaitu; lingkungan kerja (X1), pendelegasian wewenang (X2), dan bonus pegawai (X3) dengan variabel terikat yaitu kepuasan kerja pegawai (Y) BPJS Ketenagakerjaan Cabang Batam, baik secara sendiri-sendiri maupun secara bersama-sama. Populasi dari penelitian ini adalah seluruh pegawai BPJS Ketenagakerjaan Cabang Batam yang berjumlah 35 orang. Dalam penelitian ini tidak diadakan klasifikasi perbedaan pegawai berdasarkan jenis atau status golongan, kedua jenis kepegawaian tersebut mempunyai perlakuan yang sama Sampel diambil dengan menggunakan teknik simple random sampling (acak sederhana). Dengan menggunakan rumus Slovin diperoleh sampel sebanyak 32 orang. Hasil penelitian menunjukkan terdapat pengaruh positif yang signifikan lingkungan kerja (X1), pendelegasian wewenang (X2), dan bonus (X3) terhadap kepuasan kerja pegawai (Y) yang ditunjukkan oleh nilai Fhitung sebesar 23,247. Nilai ini jauh lebih kecil dari pada nilai Fhitung pada taraf signifikansi alpha 0,05 yaitu 2,95, atau F = 23,247 < F0,05(3:28) = 2,95. Pola hubungan keempat variabel yang dinyatakan oleh persamaan regresi ganda = -6,812 + 0,469X1 + 0,547X2 + 0,278X3. Sebelumnya berdasarkan uji validitas dan reliabilitas, semua instrumen memenuhi syarat validitas dan reliabilitas. Jika dihitung per variabel, maka variabel yang paling banyak berpengaruh adalah variabel pendelegasian wewenang yaitu memiliki koefisien determinasi sebesar 52,8%. Hal ini berarti pendelegasian wewenang merupakan faktor yang paling mempengaruhi kepuasan kerja pegawai.
Anatomy of the abnormal growth in the number of Russian publications in conference proceedings in Scopus
Authors: Косяков Д.В. 
Published: July 2024

Представлен детальный анализ потока публикаций с российским участием типа Conference Paper в базе данных Scopus, результаты которого ставят под сомнение некоторые кажущиеся очевидными представления о низком качестве "домашних" конференций, значимости влияния зарубежных "мусорных" конференций и других подозрительных практик на развитие этого феномена.
Many researchers have noted the phenomenon of a rapid increase in the number of publications by Russian researchers in conference proceedings indexed in international databases. This phenomenon is often associated with the general publication pressure caused by the peculiarities of the national science policy and accompanied, among other things, by systemic violations of scientific ethics. The paper provides a detailed analysis of the flow of publications with Russian participation of Conference Paper document type in the Scopus database and related conferences. Its results cast doubt on some seemingly obvious ideas about the low quality of "home" conferences, and make it possible to stumble at the significance of the influence of foreign "garbage" conferences and other suspicious practices on the development of this phenomenon.
Pengetahuan Tentang HIV/AIDS Pada Siswa Kelas XII di SMA Negeri 1 Waru
Authors: Adlina Dhiyavia Yasmin , Soelistyowati Endang , Joeliantina Anita 
Published: July 2024

ABSTRAK

Pengetahuan tentang HIV/AIDS dan sikap terhadap kondisi ini sangat penting untuk pencegahan dan pengelolaan penyebarannya. Siswa adalah salah satu kelompok yang rentan terhadap risiko penularan HIV dan memiliki potensi besar untuk menjadi agen perubahan dalam upaya pencegahan. Tujuan penelitian ini untuk mengetahui pengetahuan tentang HIV/AIDS pada siswa kelas XII di SMA Negeri 1 Waru. Jenis penelitian ini menggunakan metode penelitian deskriptif dengan pendekatan cross sectional. Pengumpulan data dengan teknik quota sampling sebanyak 151 siswa. Alat pengumpulan data yang digunakan yaitu kuesioner tentang pengetahuan HIV/AIDS yang disebarkan kepada siswa Kelas XII. Hasil dari penelitian menunjukkan bahwa hampir seluruhnya siswa kelas XII memiliki pengetahuan yang baik, sebagian kecil siswa kelas XII memiliki pengetahuan yang cukup, sebagian kecil siswa kelas XII memiliki pengetahuan kurang tentang HIV/AIDS. Hasil penelitian ini diharapkan membantu dalam mengurangi stigma dan diskriminasi terhadap individu yang hidup dengan HIV/AIDS, serta meningkatkan kesadaran dan perilaku pencegahan di kalangan siswa

Kata Kunci : Pengetahuan, Remaja, HIV/AIDS

ABSTRACT

Knowledge of HIV/AIDS and attitudes towards this condition are essential for the prevention and management of its spread. Students are one of the groups that are vulnerable to the risk of HIV transmission and have great potential to become agents of change in prevention efforts. The purpose of this study was to determine the knowledge of HIV/AIDS among XII grade students at SMA Negeri 1 Waru. This type of research uses descriptive research methods with a cross sectional approach. Data collection using quota sampling technique as many as 151 students. The data collection tool used was a questionnaire on HIV/AIDS knowledge distributed to Class XII students. The results of the study showed that almost all students of class XII had good knowledge, a small proportion of class XII students had sufficient knowledge, a small proportion of class XII students had less knowledge about HIV/AIDS. The results of this study are expected to help in reducing stigma and discrimination against individuals living with HIV/AIDS, as well as increasing awareness and preventive behavior among students.