bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Location Aware Mobile Tool for Direct and Indirect Climate Data Sensors
Authors: Ramos Francisco , Monfort Aida , Huerta Joaquin 
Published: June 2014

AbstractAccess to GIS data from mobile platforms continues to be a challenge and there is a wide range of fields where it is extremely useful. In this work, we combined three key aspects: climate data sensors, mobile platforms and spatial proximity operations. We published and made use of a web 2.0 network of climate data, where content is user‐collected, by means of their meteorological stations, and exposed as available information for the virtual community. Moreover, we enriched this data by giving the users the opportunity to directly inform the system with different climate measures. In general, management of this type of information from a mobile application could result in an important decision tool, as it enables us to provide climate‐related data according to a context and a geographical location. Therefore, we implemented a native mobile application for iPhone and iPad platforms by using ArcGIS SDK for iOS and by integrating a series of ArcGIS webmaps, which allows us to perform geospatial queries based on the user's location, offering, at the same time, access to all the data provided by the climate data sensor network and from direct users.
The BEL1‐type homeobox gene SH5 induces seed shattering by enhancing abscission‐zone development and inhibiting lignin biosynthesis
Authors: Yoon Jinmi , Cho Lae‐Hyeon , Kim Song Lim , Choi Heebak , Koh Hee‐Jong , An Gynheung 
Published: June 2014

SummarySeed shattering is an important trait that influences grain yield. A major controlling quantitative trait locus in rice is qSH1. Although the degree of shattering is correlated with the level of expression of qSH1, some qSH1‐defective cultivars display moderate shattering while others show a non‐shattering phenotype. Os05 g38120 (SH5) on chromosome 5 is highly homologous to qSH1. Although we detected SH5 transcripts in various organs, this gene was highly expressed at the abscission zone (AZ) in the pedicels. When expression of this gene was suppressed in easy‐shattering ‘Kasalath’, development of the AZ was reduced and thereby so was seed loss. By contrast, the extent of shattering, as well as AZ development, was greatly enhanced in moderate‐shattering ‘Dongjin’ rice when SH5 was overexpressed. Likewise, overexpression of SH5 in the non‐shattering ‘Ilpum’ led to an increase in seed shattering because lignin levels were decreased in the basal region of spikelets in the absence of development of an AZ. We also determined that two shattering‐related genes, SHAT1 and Sh4, which are necessary for proper formation of an AZ, were induced by SH5. Based on these observations, we propose that SH5 modulates seed shattering by enhancing AZ development and inhibiting lignin biosynthesis.
Cloning of theArabidopsisrwm1gene for resistance toWatermelon mosaic viruspoints to a new function for natural virus resistance genes
Authors: Ouibrahim Laurence , Mazier Marianne , Estevan Joan , Pagny Gaëlle , Decroocq Véronique , Desbiez Cécile , Moretti André , Gallois Jean‐Luc , Caranta Carole 
Published: June 2014

SummaryArabidopsis thalianarepresents a valuable and efficient model to understand mechanisms underlying plant susceptibility to viral diseases. Here, we describe the identification and molecular cloning of a new gene responsible for recessive resistance to several isolates ofWatermelon mosaic virus(WMV, genusPotyvirus) in the Arabidopsis Cvi‐0 accession.rwm1acts at an early stage of infection by impairing viral accumulation in initially infected leaf tissues. Map‐based cloning delimitedrwm1on chromosome 1 in a 114‐kb region containing 30 annotated genes. Positional and functional candidate gene analysis suggested thatrwm1encodescPGK2 (At1g56190), an evolutionary conserved nucleus‐encoded chloroplast phosphoglycerate kinase with a key role in cell metabolism. Comparative sequence analysis indicates that a single amino acid substitution (S78G) in the N‐terminal domain ofcPGK2 is involved inrwm1‐mediated resistance. This mutation may have functional consequences because it targets a highly conserved residue, affects a putative phosphorylation site and occurs within a predicted nuclear localization signal. Transgenic complementation in Arabidopsis together with virus‐induced gene silencing inNicotiana benthamianaconfirmed thatcPGK2corresponds torwm1and that the protein is required for efficient WMV infection. This work uncovers new insight into natural plant resistance mechanisms that may provide interesting opportunities for the genetic control of plant virus diseases.
Two CCAAT‐box‐binding transcription factors redundantly regulate early steps of the legume‐rhizobia endosymbiosis
Authors: Laloum Tom , Baudin Maël , Frances Lisa , Lepage Agnes , Billault‐Penneteau Benjamin , Cerri Marion R. , Ariel Federico , Jardinaud Marie‐Françoise , Gamas Pascal , de Carvalho‐Niebel Fernanda , Niebel Andreas 
Published: June 2014

SummaryDuring endosymbiotic interactions between legume plants and nitrogen‐fixing rhizobia, successful root infection by bacteria and nodule organogenesis requires the perception and transduction of bacterial lipo‐chitooligosaccharidic signal called Nod factor (NF). NF perception in legume roots leads to the activation of an early signaling pathway and of a set of symbiotic genes which is controlled by specific early transcription factors (TFs) including CYCLOPS/IPD3, NSP1, NSP2, ERN1 and NIN. In this study, we bring convincing evidence that the Medicago truncatula CCAAT‐box‐binding NF‐YA1 TF, previously associated with later stages of rhizobial infection and nodule meristem formation is, together with its closest homolog NF‐YA2, also an essential positive regulator of the NF‐signaling pathway. Here we show that NF‐YA1 and NF‐YA2 are both expressed in epidermal cells responding to NFs and their knock‐down by reverse genetic approaches severely affects the NF‐induced expression of symbiotic genes and rhizobial infection. Further over‐expression, transactivation and ChIP‐PCR approaches indicate that NF‐YA1 and NF‐YA2 function, at least in part, via the direct activation of ERN1. We thus propose a model in which NF‐YA1 and NF‐YA2 appear as early symbiotic regulators acting downstream of DMI3 and NIN and possibly within the same regulatory complexes as NSP1/2 to directly activate the expression of ERN1.
Draft Genome Sequences of Two Clinical Isolates of Streptococcus mutans
Authors: Zheng Hui , Guo Lihong , Du Ning , Lin Jiuxiang , Song Lai , Liu Guiming , Chen Feng 
Published: June 2014

ABSTRACT

We report the draft genome sequences of PKUSS-HG01 and PKUSS-LG01, two clinical isolates of
Streptococcus mutans
from human dental plaque. The genomics information will facilitate the study of the mechanisms of pathogenicity and evolution of
S. mutans
.
Genome Sequences of Porcine Epidemic Diarrhea Virus:
In Vivo
and
In Vitro
Phenotypes
Authors: Lawrence Paulraj K. , Bumgardner Eric , Bey Russell F. , Stine Douglas , Bumgarner Roger E. 
Published: June 2014

ABSTRACT

Since the outbreak of porcine epidemic diarrhea virus (PEDV) in May 2013, U.S. swine producers have lost almost five million baby pigs. In an attempt to understand the evolution of PEDV in the United States and possibly develop a control strategy, we compared the genome sequences of a PEDV strain isolated from an infected piglet against its
in vitro
adapted version. The original PEDV strain was grown in Vero cells and passed 10 times serially in a MARC145 cell line. The sequence analysis of the native PEDV strain and
in vitro
passaged virus shows that the cell culture adaptation specifically modifies PEDV spike protein whereas the open reading frame 1a/b (ORF1a/b)-encoded polyprotein, the nucleoprotein, NS3B (ORF3), and membrane and envelope proteins remain unchanged.
Genome Sequence of SCB34, a Sequence Type 131 Multidrug-Resistant Escherichia coli Isolate Causing Neonatal Early-Onset Sepsis
Authors: Chavez-Bueno Susana , Day Michael W. , Toby Inimary T. , Akins Darrin R. , Dyer David W. 
Published: June 2014

ABSTRACT

SCB34 is a sequence type 131, highly invasive, multidrug-resistant
Escherichia coli
isolate that produced neonatal bacteremia. Whole-genome sequencing was performed using a 250-bp library on the Illumina MiSeq platform; 5,910,264 reads were assembled
de novo
using the A5 assembly pipeline. The total contig length was 5,227,742 bp; the RAST server was used for annotation.
Genetic Characterization of HIV-1 Subtype D Near-Full-Length Proviral Genomes by Illumina Massively Parallel Sequencing Technology
Authors: Pessôa Rodrigo , Lopes Maria Esther , Sanabani Sabri S. 
Published: June 2014

ABSTRACT
This study describes the near-full-length genome deep sequencing of two HIV-1 subtype D strains identified in blood donors in Rio de Janeiro, Brazil, in what seems to have been a small restricted subtype D epidemic in the country.
Acupuncture for Treatment of Secondary Osteoporosis in Patients with Spinal Cord Injury: A Controlled Study
Authors: Meng Qingxi , Liu Xin , Shan Qunqun , Yu Peng , Mao Zhaohu , Zhang Fan , Li Jian , Zhao Tingbao 
Published: June 2014

Objective We explored the effect of adjunctive acupuncture on secondary osteoporosis in patients with spinal cord injury (SCI). Methods Patients with subacute SCI were recruited and divided into two groups by patient choice: group 1 patients received standard combination therapy and group 2 patients received combination therapy plus acupuncture for 3 months. The concentrations of IgG, IgM and tumour necrosis factor α (TNFα) in serum and the bone mineral density were measured before and after treatment. Result The decrease in the concentration of TNFα and IgM in patients in group 2 compared with those in group 1 was statistically significant. The IgG level showed no significant change in either group. Bone mineral density increased more after adjunctive acupuncture, but the difference was not significant. Conclusions Further research is needed to determine whether acupuncture as an adjunct to combination therapy can reduce osteoporosis in patients with subacute SCI. Trial Registration Number P153-2008-36
The Early Detection of Pancreatic Cancer: What Will It Take to Diagnose and Treat Curable Pancreatic Neoplasia?
Authors: Lennon Anne Marie , Wolfgang Christopher L. , Canto Marcia Irene , Klein Alison P. , Herman Joseph M. , Goggins Michael , Fishman Elliot K. , Kamel Ihab , Weiss Matthew J. , Diaz Luis A. , Papadopoulos Nickolas , Kinzler Kenneth W. , Vogelstein Bert , Hruban Ralph H. 
Published: June 2014

Abstract
Pancreatic cancer is the deadliest of all solid malignancies. Early detection offers the best hope for a cure, but characteristics of this disease, such as the lack of early clinical symptoms, make the early detection difficult. Recent genetic mapping of the molecular evolution of pancreatic cancer suggests that a large window of opportunity exists for the early detection of pancreatic neoplasia, and developments in cancer genetics offer new, potentially highly specific approaches for screening of curable pancreatic neoplasia. We review the challenges of screening for early pancreatic neoplasia, as well as opportunities presented by incorporating molecular genetics into these efforts. Cancer Res; 74(13); 3381–9. ©2014 AACR.
Validation of Plasma Proneurotensin as a Novel Biomarker for the Prediction of Incident Breast Cancer
Authors: Melander Olle , Belting Mattias , Manjer Jonas , Maisel Alan S. , Hedblad Bo , Engström Gunnar , Nilsson Peter , Struck Joachim , Hartmann Oliver , Bergmann Andreas , Orho-Melander Marju 
Published: June 2014

Abstract
Background: High fasting plasma proneurotensin concentration was associated with the development of breast cancer in the Malmö Diet and Cancer Study (MDCS). Here, we aimed at replicating the initial finding in an independent second cohort.
Methods: The Malmö Preventive Project (MPP) is a population study and comprised 18,240 subjects when examined in 2002–2006. Of women without history of breast cancer at examination, we included all who developed breast cancer during follow-up (n = 130) until December 31, 2010, and a random sample of women without breast cancer until the end of follow-up (n = 1,439) for baseline plasma proneurotensin assessment (mean age, 70.0 ± 4.4 years). Proneurotensin was measured in fasting plasma samples and was related to the risk of later breast cancer development using multivariate logistic regression.
Results: Proneurotensin [odds ratio (OR) per standard deviation (SD) increment of LN-transformed proneurotensin] was significantly related to incident breast cancer [OR, 2.09; 95% confidence interval (CI), 1.79–2.44; P &lt; 0.001; adjusted for age, body mass index (BMI), smoking, and hormone replacement therapy]. The effect estimate in the MPP was larger than in the discovery cohort (MDCS), with the main difference between the two cohorts being that women of the MPP study were on the average about 10 years older and follow-up time was shorter than that of the MDCS.
Conclusion: As initially found in the MDCS, fasting plasma proneurotensin was significantly associated with the development of breast cancer in the MPP study as well.
Impact: Measurement of plasma proneurotensin warrants further investigation as a blood-based marker for early breast cancer detection. Cancer Epidemiol Biomarkers Prev; 23(8); 1672–6. ©2014 AACR.
Chloroquine Inhibits Autophagy to Potentiate Antiestrogen Responsiveness in ER+ Breast Cancer
Authors: Cook Katherine L. , Wärri Anni , Soto-Pantoja David R. , Clarke Pamela AG. , Cruz M. Idalia , Zwart Alan , Clarke Robert 
Published: June 2014

Abstract
Purpose: Estrogen receptor-α (ERα)-targeted therapies including tamoxifen (TAM) or Faslodex (ICI) are used to treat ER+ breast cancers. Up to 50% of tumors will acquire resistance to these interventions. Autophagy has been implicated as a major driver of antiestrogen resistance. We have explored the ability of chloroquine (CQ), which inhibits autophagy, to affect antiestrogen responsiveness.
Experimental Design: TAM-resistant MCF7-RR and ICI-resistant/TAM cross-resistant LCC9 ER+ breast cancer cells were injected into mammary fat pads of female athymic mice and treated with TAM and/or ICI in combination with oral low-dose CQ.
Results: We show that CQ can increase antiestrogen responsiveness in MCF7-RR and LCC9 cells and tumors, likely through the inhibition of autophagy. However, the combination of ICI+CQ was less effective than CQ alone in vivo, unlike the TAM+CQ combination. Antiestrogen treatment stimulated angiogenesis in tumors but did not prevent CQ effectiveness. The lower efficacy of ICI+CQ was associated with ICI effects on cell-mediated immunity within the tumor microenvironment. The mouse chemokine KC (CXCL1) and IFNγ were differentially regulated by both TAM and ICI treatments, suggesting a possible effect on macrophage development/activity. Consistent with these observations, TAM+CQ treatment increased tumor CD68+ cells infiltration, whereas ICI and ICI+CQ reduced peripheral tumor macrophage content. Moreover, macrophage elimination of breast cancer target cells in vitro was reduced following exposure to ICI.
Conclusion: CQ restores antiestrogen sensitivity to resistant tumors. Moreover, the beneficial combination of TAM+CQ suggests a positive outcome for ongoing neoadjuvant clinical trials using this combination for the treatment of ER+ ductal carcinoma in situ lesions. Clin Cancer Res; 20(12); 3222–32. ©2014 AACR.
Strategic Planning to Reduce the Burden of Stroke Among Veterans
Authors: Lich Kristen Hassmiller , Tian Yuan , Beadles Christopher A. , Williams Linda S. , Bravata Dawn M. , Cheng Eric M. , Bosworth Hayden B. , Homer Jack B. , Matchar David B. 
Published: June 2014

Background and Purpose—
Reducing the burden of stroke is a priority for the Veterans Affairs Health System, reflected by the creation of the Veterans Affairs Stroke Quality Enhancement Research Initiative. To inform the initiative’s strategic planning, we estimated the relative population-level impact and efficiency of distinct approaches to improving stroke care in the US Veteran population to inform policy and practice.

Methods—
A System Dynamics stroke model of the Veteran population was constructed to evaluate the relative impact of 15 intervention scenarios including both broad and targeted primary and secondary prevention and acute care/rehabilitation on cumulative (20 years) outcomes including quality-adjusted life years (QALYs) gained, strokes prevented, stroke fatalities prevented, and the number-needed-to-treat per QALY gained.

Results—
At the population level, a broad hypertension control effort yielded the largest increase in QALYs (35 517), followed by targeted prevention addressing hypertension and anticoagulation among Veterans with prior cardiovascular disease (27 856) and hypertension control among diabetics (23 100). Adjusting QALYs gained by the number of Veterans needed to treat, thrombolytic therapy with tissue-type plasminogen activator was most efficient, needing 3.1 Veterans to be treated per QALY gained. This was followed by rehabilitation (3.9) and targeted prevention addressing hypertension and anticoagulation among those with prior cardiovascular disease (5.1). Probabilistic sensitivity analysis showed that the ranking of interventions was robust to uncertainty in input parameter values.

Conclusions—
Prevention strategies tend to have larger population impacts, though interventions targeting specific high-risk groups tend to be more efficient in terms of number-needed-to-treat per QALY gained.
Incidental Magnetic Resonance Diffusion-Weighted Imaging–Positive Lesions Are Rare in Neurologically Asymptomatic Community-Dwelling Adults
Authors: Batool Saima , O’Donnell Martin , Sharma Mukul , Islam Shofiqul , Dagenais Gilles R. , Poirier Paul , Lear Scott A. , Wielgosz Andreas , Teo Koon , Stotts Grant , McCreary Cheryl R. , Frayne Richard , DeJesus Jane , Rangarajan Sumathy , Yusuf Salim , Smith Eric E. 
Published: June 2014

Background and Purpose—
Incidental magnetic resonance diffusion-weighted imaging (DWI)–positive lesions, considered to represent small acute infarcts, have been detected in patients with cerebral small vessel diseases or cognitive impairment, but the prevalence in the community population is unknown.

Methods—
DWI sequences collected in 793 participants in the Prospective Urban Rural Epidemiological (PURE) study were reviewed for DWI lesions consistent with small acute infarcts.

Results—
No DWI-positive lesions were detected (0%, 95% confidence interval, 0–0.5).

Conclusions—
DWI-positive lesions are rare in an asymptomatic community population. The prevalence of DWI-positive lesions in the community seems to be lower than in patients with cerebral amyloid angiopathy, intracerebral hemorrhage, or cognitive impairment.
Randomized Controlled Trial on Hemifield Eye Patching and Optokinetic Stimulation in Acute Spatial Neglect
Authors: Machner Björn , Könemund Inga , Sprenger Andreas , von der Gablentz Janina , Helmchen Christoph 
Published: June 2014

Background and Purpose—
Right hemisphere stroke patients frequently experience spatial neglect, a severe lack of awareness for contralesional hemispace. Although neglect counts among the strongest predictors for poor functional outcome after stroke, there is no established therapy, particularly not for the acute stage.

Methods—
In a randomized controlled trial, we compared the combined treatment of hemifield eye patching and repetitive optokinetic stimulation in acute stroke patients with neglect to the spontaneous course. Outcome measures were a neuropsychological test battery for neglect as well as scales of functional independence and clinical impairment. Outcomes were assessed at baseline (day 1), post treatment (day 8), and at 1-month follow-up (day 30).

Results—
Final analysis included 21 acute right hemisphere stroke patients with neglect (23 enrolled, 2 lost to follow-up) allocated either to the treatment (1 week hemifield eye patching and daily sessions of optokinetic stimulation, n=11) or the control group (no neglect-specific treatment, n=10). At baseline, both groups did not differ in neuropsychological test performance, clinical impairment, or functional disability. At the post treatment session, both groups had improved in all these measures, and results were stable or further improved at follow-up. However, there was no significant difference in this change between the treatment and the control group.

Conclusions—
An early intervention of combined hemifield eye patching and optokinetic stimulation in acute stroke patients with spatial neglect has no additive effect to the spontaneous remitting course of the disorder.

Clinical Trial Registration—

URL:
http://www.clinicaltrials.gov
. Unique identifier: NCT01617343.
<span lang="EN-GB">Aristolochia gurinderii</span><span lang="EN-GB"> (Aristolochiaceae): a new species from Great Nicobar Island, India</span>
Authors: Ravikumar K , Tiwari Umeshkumar L , Balachandran N 
Published: June 2014

Aristolochia gurinderiidescribed here as a new species, was collected from North-South Road, Campbell Bay, Great Nicobar Island (Andaman and Nicobar Islands), India. Stem woody with corky fissured bark; lamina deltoid or hastate; cauliflorous inflorescences in scorpioid cyme up to 55 cm long, small size of flowers; seeds winged with distinctive ridge at the center are the distinguishable characters from its allied species A. jackii Steudel. With a detailed description, illustration, photo plates are provided.
Summer Arctic Atmospheric Circulation Response to Spring Eurasian Snow Cover and Its Possible Linkage to Accelerated Sea Ice Decrease
Authors: Matsumura Shinji , Zhang Xiangdong , Yamazaki Koji 
Published: June 2014

Abstract
Anticyclonic circulation has intensified over the Arctic Ocean in summer during recent decades. However, the underlying mechanism is, as yet, not well understood. Here, it is shown that earlier spring Eurasian snowmelt leads to anomalously negative sea level pressure (SLP) over Eurasia and positive SLP over the Arctic, which has strong projection on the negative phase of the northern annular mode (NAM) in summer through the wave–mean flow interaction. Specifically, earlier spring snowmelt over Eurasia leads to a warmer land surface, because of reduced surface albedo. The warmed surface amplifies stationary Rossby waves, leading to a deceleration of the subpolar jet. As a consequence, rising motion is enhanced over the land, and compensating subsidence and adiabatic heating occur in the Arctic troposphere, forming the negative NAM. The intensified anticyclonic circulation has played a contributing role in accelerating the sea ice decline observed during the last two decades. The results here provide important information for improving seasonal prediction of summer sea ice cover.