bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing time from presentation to surgical intervention for testicular torsion: implementation of a quality improvement protocol
Authors: Richardson Shannon , Huen Kathy , Benga Tabitha , Fajardo Bridgette , Sturm Renea , Lerman Steven E. , Singer Jennifer S. 
Published: July 2024

IntroductionTimely surgical intervention for patients with testicular torsion is a quality benchmark set by the U.S. News and World Report (USNWR) for pediatric urology. In this study, we describe and evaluate a quality improvement initiative to reduce the time to surgical intervention for testicular torsion at a single institution through the implementation of a clinical care pathway called “code torsion.”MethodsData abstraction was performed through retrospective chart review to assess process measures. Patients &lt;21 years old with testicular torsion requiring surgical intervention were included. The clinical protocol “code torsion” was created by a multidisciplinary quality improvement workgroup with the primary goal of reducing the time from emergency department presentation to surgical intervention for testicular torsion. “Code torsion” was implemented in October 2021, which was assessed in addition to subsequent interventions through plan–do–study–act (PDSA) cycles.ResultsA total of 30 patients were identified prior to “code torsion” implementation and 14 thereafter. The mean time from triage to operating room (OR) was 228 min prior to “code torsion” compared with 180 min after protocol implementation (p = 0.047). The proportion of cases that had surgical intervention within the 4-h USNWR metric increased from 63% pre-protocol to 93% post-protocol (p = 0.07). Of the patients, 40% required orchiectomy prior to “code torsion” compared with 29% after implementation (p = 0.5). Patients requiring orchiectomy had a significantly longer time from symptom onset to surgical intervention (87 vs. 9.8 h, p &lt; 0.001).ConclusionImplementation of the protocol “code torsion” was successful in reducing the time from presentation to surgical intervention for testicular torsion. The rates of testicular salvage did not differ after “code torsion” implementation and were instead found to be dependent on the total ischemia time.
Implementation of Spore Display in Paenibacillus polymyxa with Different Hydrolytic Enzymes
Authors: Zander Maximilian , Schmid Jochen , Kabisch Johannes 
Published: July 2024

Biotechnological processes are essential for producing climate-friendly high-value chemicals or pharmaceutical compounds, which can include steps catalyzed by enzymes. Therefore, establishing new, robust, and cheap enzyme production processes is desirable. One possible way to enhance processes is through the use of the spore display method. Spore display can present heterologous proteins on the surface of bacterial spores, offering numerous advantages in a range of biotechnological applications. This study demonstrates the implementation of the spore display method in Paenibacillus polymyxa, achieved by modifying the spore surface, incorporating an anchoring protein, and attaching green fluorescent protein to it, allowing the visualization of fluorescent spores. Following the initial experiment, a native lipase (Lip3), a heterologous lipase (LipA) from Bacillus subtilis, a native esterase (PnbA) from P. polymyxa, and a lipoyl synthase were expressed during sporulation and displayed on the spore surface. The activity profiles were determined in the temperature range from 4 °C to 70 °C. The PnbA reached its optimum at 4 °C, whereas the LipA from B. subtilis showed 4.4-fold higher activity at 42 °C compared to the control. Furthermore, we explored a possible new technique for the purification of enzymes with the TEV cleavage site between the anchor and the protein of interest. Finally, we showed a not-yet-described side activity of the lipoyl synthase over a wide temperature range.
Aggravated Ulcerative Colitis via circNlgn-Mediated Suppression of Nuclear Actin Polymerization
Authors: Du William W. , Zhou Chi , Yang Hui , Wen Shuoyang , Chen Yu , Chen Eric X. , Yang Xiuwei H. , Li Feiya , Du Kevin Y. , Yuan Hui , Ye Ting , Qadir Javeria , Yang Burton B. 
Published: July 2024

Colitis is a chronic bowel disease characterized by damage to the lining of the large intestine, with its precise underlying causes remaining incompletely understood. In this study, we provide evidence that circular RNA circNlgn plays a pivotal role in promoting the development of colitis. Colitis patients produce significant higher levels of circNlgn. Transgenic mice expressing circNlgn exhibit heightened susceptibility to colitis development and progression, primarily attributed to the presence of the protein isoform Nlgn173 encoded by circNlgn. Nlgn173 undergoes translocation into cell nuclei, where it interacts with actin, impeding the binding of actin-related protein 2 and 3 (Arp2/3) complex to actin molecules. Consequently, this leads to a reduction in actin polymerization. Mechanistically, Nlgn173 enhances tyrosine-53 phosphorylation of nuclear actin, diminishing its capacity to interact with the Arp2/3 complex and causing a decrease in filamentous actin levels. These alterations in actin dynamics result in inhibited cell cycle progression, increased apoptosis, and decreased proliferation of colonic epithelial cells, thereby exacerbating colitis development and progression. In contrast, the silencing of circNlgn or the targeted inhibition of Nlgn173 translation and nuclear translocation leads to the promotion of nuclear actin polymerization, enhanced cell survival, and reduced apoptosis and ultimately improves the outcome of colitis in vivo. Interestingly, nuclear actin polymerization is highly related with expression of PIAS3, which modulates signal transducer and activator of transcription 3 and NF-κB activity in colitis. Strategies such as circNlgn knockdown and targeting nuclear actin polymerization of the colonic epithelium may explore a novel avenue for acute ulcerative colitis clinical intervention.
Anti-TNFR2 Antibody-Conjugated PLGA Nanoparticles for Targeted Delivery of Adriamycin in Mouse Colon Cancer
Authors: Li Ping , Yang Yang , Wang Yifei , Zheng Jingbin , Chen Fengyang , Jiang Mengmeng , Chou Chon-kit , Cong Weihong , Li Zongjin , Chen Xin 
Published: July 2024

High levels of tumor necrosis factor receptor type II (TNFR2) are preferentially expressed by immunosuppressive CD4
+
Foxp3
+
regulatory T cells (T
regs
), especially those present in the tumor microenvironment, as initially reported by us. There is compelling evidence that targeting TNFR2 markedly enhances antitumor immune responses. Furthermore, a broad spectrum of human cancers also expresses TNFR2, while its expression by normal tissue is very limited. We thus hypothesized that TNFR2 may be harnessed for tumor-targeted delivery of chemotherapeutic agents. In this study, we performed a proof-of-concept study by constructing a TNFR2-targeted PEGylated poly(
dl
-lactic-co-glycolic acid) (PLGA-PEG) nanodrug delivery system [designated as TNFR2-PLGA-ADR (Adriamycin)]. The results of in vitro study showed that this TNFR2-targeted delivery system had the properties in cellular binding and cytotoxicity toward mouse colon cancer cells. Further, upon intravenous injection, TNFR2-PLGA-ADR could efficiently accumulate in MC38 and CT26 mouse colon tumor tissues and preferentially bind with tumor-infiltrating T
regs
. Compared with ADR and ISO-PLGA-ADR, the in vivo antitumor effect of TNFR2-PLGA-ADR was markedly enhanced, which was associated with a decrease of TNFR2
+
T
regs
and an increase of IFNγ
+
CD8
+
cytotoxic T lymphocytes in the tumor tissue. Therefore, our results clearly show that targeting TNFR2 is a promising strategy for designing tumor-specific chemoimmunotherapeutic agent delivery system.
Psychological Aspects of Awake Surgery in Pediatric Neurosurgery
Authors: Талабаев М.В. , Чурило Н.В. , Соловьева А.Ю. 
Published: July 2024

Цель. Изучить психоэмоциональную переносимость операций в сознании у детей и выявить признаки развития посттравматического стрессового расстройства. Материалы и методы. Проанализированы психоэмоциональные аспекты, связанные с перенесенной операцией в сознании по поводу новообразований, расположенных в функционально значимых отделах коры головного мозга у 17 пациентов, проходивших обследование и нейрохирургическое лечение в РНПЦ неврологии и нейрохирургии с 2016 по 2022 год. В процессе исследования для оценки психоэмоционального состояния была разработана психометрическая шкала Лайкерта. Результаты. Анализ заполнения шкал Лайкерта показал, что до операции пациентов с высоким уровнем баллов психоэмоционального состояния было 7 (46,6%) человек, средним – 4 (26,7%), низким – 4 (26,7%) человека. Большинство, 73,3% пациентов, находились в достаточно комфортном психоэмоциональном состоянии. Послеоперационное тестирование, проведенное через 9–12 месяцев после операции, показало, что высокий уровень сохранился у 6 (40%) испытуемых, средний у 6 (40%), низкий у 3 (20%). Заключение. Наши результаты показывают, что операции в сознании у пациентов детского возраста безопасны и не явились значительным психотравмирующим событием.
Purpose. To study the psycho-emotional tolerance of awake operations in children and to identify signs of the development of post-traumatic stress disorder. Materials and methods. The psycho-emotional aspects associated with undergoing awake surgery for eloquent brain tumors were analyzed in 17 patients who underwent examination and neurosurgical treatment at the Republican Scientific and Practical Center of Neurology and Neurosurgery from 2016 to 2022. During the study, a psychometric Likert scale was developed to assess the psycho-emotional state. Results. Analysis of the completion of Likert scales showed that before surgery there were 7 (46.6%) patients with a high level of scores, 4 (26.7%) with an average level of scores, and 4 (26.7%) with a low level of scores. The majority of 73.3% of patients were in a fairly comfortable psycho-emotional state. Postoperative testing, carried out 9–12 months after surgery, showed that the high level remained in 6 (40%) subjects, medium in 6 (40%), low in 3 (20%). Conclusion. Our results demonstrate that awake surgery in pediatric patients is safe and was not a significant traumatic incident.
Drug-Resistant Epilepsy: Current State of the Problem and Prospects Treatment in Belarus
Authors: Марьенко И.П. , Лихачев С.А. , Костюкевич Е.П. , Терехов В.С. 
Published: July 2024

Введение. Проблема фармакорезистентной эпилепсии (ФРЭ) остается на сегодняшний день до конца нерешенной. Электростимуляция блуждающего нерва относится к немедикаментозным видам терапии ФРЭ. Стимулятор блуждающего нерва (vagus nerve stimulator – VNS) используется в качестве вспомогательного метода лечения для снижения частоты приступов у пациентов с ФРЭ. Цель. Оценить результаты VNS-терапии у пациентов с ФРЭ, находящихся под динамическим наблюдением с 2014 по 2024 г. Материалы и методы. В исследование были включены 53 пациента (мужчин – 22 (42%), женщин – 31 (58%), средний возраст 31 [33,7; 28,4] год) с ФРЭ и с имплантированной VNS-системой, находящихся под динамическим наблюдением с 2014 по 2024 г. Результаты. Применение VNS-терапии ФРЭ демонстрирует снижение частоты эпиприступов более чем у трети пациентов – в 19 (36%) случаях из 53, из них в 8 (15%) случаях наблюдалось снижении частоты серий приступов. Заключение. Полученные результаты демонстрируют перспективность использования VNS-терапии.
Introduction. The problem of Drug-resistant epilepsy (DRE) remains today is not fully resolved. Electrical stimulation of the vagus nerve is a non-drug type of EEG therapy. Vagus nerve stimulator VNS therapy is used as an adjuvant treatment to reduce the frequency of attacks in patients with EEG. Purpose. To evaluate the results of VNS therapy in patients with EEG who were under dynamic observation from 2014 to 2024. Materials and methods. The study included 53 patients (men – 22 (42%), women – 31 (58%), average31 [33.7; 28.4] years) whit DRE and an implanted VNS system, who were under dynamic observation from 2014 to 2024. Results. The use of VNS-therapy DRE demonstrates a decrease in the freguency of epileptic attacks in more than a third of patients in 19 (36%) cases out of 53, of which in 8 (15%) cases there was a decrease in the frequency of series of attacks. Conclusion. The results obtained show promise use of VNS therapy.
ANALISIS MANAJEMEN RISIKO KEAMANAN DAN KUALITAS PENGGUNAAN PERALATAN MEDIS DI RUMAH SAKIT UMUM DAERAH ARIFIN ACHMAD PROVINSI RIAU
Authors: Lasiyah Nani , Romi Mulyadi  , Okta Nola Yolanda iriani  
Published: July 2024

Peralatan elektro-medis memegang peran penting dalam penyelenggaraan pelayanan kesehatan masyarakat. Pelayanan kesehatan harus didukung oleh perangkat yang tersedia dan berfungsi sesuai UU RI Nomor 56 Tahun 2014 . Penelitian ini bertujuan menganalisis manajemen risiko terkait peralatan elektro-medis menjamin penggunaan yang aman dan tidak terjadi kecelakaan kerja selama penggunaan baik bagi pasien maupun tenaga medis lainnya sesuai ISO 14971:2019. Metode penelitian kuantitatif dengan pendekatan studi dokumen, lembar ceklist dan wawancara. Berdasarkan hasil penelitian yang dilakukan manajemen risiko peralatan elektro-medis di RSUD Arifin Achmad telah dilaksanakan dengan baik dan sesuai standar ISO 14971:2019 didapatkan presentase kesesuaian manajemen risiko peralatan elektro-medis 100%. Terdapat klasifikasi risiko peralatan elektro-medis low, medium, dan high. Evaluasi risiko dilakukan dengan labelisasi alat medis berbahaya, dan pengendalian risiko dilakukan melalui langkah-langkah preventif, pemeliharaan rutin, serta kalibrasi alat medis. Dokumentasi risiko terdapat kendala anggaran dan biaya dalam proses manajemen risiko peralatan elektro-medis. Berdasarkan dari data yang diperoleh dapat ditarik kesimpulan manajemen risiko peralatan elektro-medis di RSUD Arifin Achmad telah sesuai ISO 14971:2019 bahwa 100% sesuai.
KOMBINASI PUPUK KANDANG KAMBING DAN KALIUM NITRAT (KNO3) TERHADAP PERTUMBUHAN DAN KECEPATAN BERBUNGA JAGUNG MANIS (Zea mays saccharata)
Authors: Fitriyah Nur , Rahmatika Widyana , Melya Contesya Sherlin 
Published: July 2024

Zea mays Saccharata (Sweet corn) is a horticultural commodity that is have high carbohydrates and contains free sugars and starch (Wahditiya, 2016). sweet corn imports increased 42.46% from 517.5 thousand tons in the previous year to 737.2 thousand tons (BPS, 2021 in Amelia and Sugiharto, 2022). This high demand is not matched by availability. Efforts to meet this demand include improving cultivation techniques such as used of superior varieties and ecological management. The combination of organic and anorganic fertilizers can play an important role because it can maintain soil health, productivity and plant fertility. The combination of goat fertilizer and KNO3 is expected to increase vegetative and generative performance. The research method used RAKF. The first factor is the dose of goat manure, K1 = 5 tons/ha, K2 = 10 tons/ha, K3 = 15 tons/ha. The second factor is the application of white KNO3 fertilizer P1 = 50 kg/ha, P2 = 100 kg/ha, P3 = 150 kg/ha. There were 9 treatment combinations and repeated 3 times and 5 samples. The observed parameters include: Plant height, number of leaves, leaf area, and flowering age. Data obtained from the observations of each variable were subjected to the F test with the method of variance (ANOVA). If the treatment combination accepted H1 then the comparison test will be done BNT 5%. The results showed that there was no interaction between the application of goat manure and KNO3 on the growth and yield. The application of KNO3 has a significant effect on the vegetative period of the plant number of leaves, plant height, stem diameter, and early flowering age of the plant.
Hubungan Tingkat Pengetahuan dengan Sikap Pria terhadap Penggunaan Kontrasepsi Kondom di Kecamatan Tuminting Tahun 2023
Authors: Rampengan Derren D. C. H. , Turalaki Grace L. A. , Tendean Lidya E. N. 
Published: July 2024

Abstract: The government's efforts to control the rate of population growth is inter alia the family planning program using contraceptives; one of the them is condom. This study aimed to determine the relationship between men’s knowledge and attitudes with condom use at Tuminting in 2023. This was a quantitative and descriptive study with a cross-sectional design. Data were analyzed using the Pearson correlation test and regression analysis. The results showed that there were 394 men as respondents. Knowledge and attitude scores were higher in the group using condoms (46.8±0.79 and 20.11±2.39). Based on education level, the highest knowledge score was in the respondents of senior high school (4.67±0.91). Meanwhile, the highest behavior score was in the respondents of elementary school education (21.80±2.59). The correlation test obtained an r-value of 0.542 and a p-value of <0.005, indicating that there was a positive moderate significant correlation between knowledge and attitude towards the use of condom as contraceptive. In conclusion, there is a significant positive correlation between knowledge and attitude towards condom use among men at Tuminting in 2023.
Keywords: condom contraception; knowledge; attitude

Abstrak: Upaya pemerintah untuk mengendalikan laju pertumbuhan pendudukan ialah antara lain melalui program Keluarga Berencana (KB) dengan menggunakan kontrasepsi; salah satunya ialah penggunaan kondom. Penelitian ini bertujuan untuk mengetahui hubungan antara pengetahuan dan sikap pria dengan penggunaan kontrasepsi kondom di Kecamatan Tuminting tahun 2023. Jenis penelitian ialah kuantitatif dengan pendekatan deskriptif analitik dan desain potong lintang. Uji statistik menggunakan uji korelasi Pearson dan regresi linier. Hasil penelitian mendapatkan 394 pria dewasa sebagai responden. Skor pengetahuan dan perilaku lebih tinggi pada kelompok responden yang menggunakan kontrasepsi kondom (46,8±0,79 dan 20,11±2,39). Berdasarkan tingkat pendidikan, skor pengetahuan paling tinggi berada pada kelompok responden dengan tingkat pendidikan tertinggi SMA/SMK (4,67±0,91), sedangkan skor perilaku paling tinggi berada pada kelompok responden dengan tingkat pendidikan SD (21,80±2,59). Hasil uji korelasi Pearson mendapatkan nilai r=0,542 dengan nilai p<0,005, yang menunjukkan terdapat korelasi positif kekuatan sedang yang bermakna antara pengetahuan dengan sikap mengenai penggunaan kontrasepsi kondom. Simpulan penelitian ini ialah terdapat korelasi positif yang bermakna antara pengetahuan dengan sikap mengenai penggunaan kontrasepsi kondom pada pria di Kecamatan Tuminting tahun 2023.
Kata kunci: kontrasepsi kondom; pengetahuan; sikap
Analisis Kebijakan Pengelolaan Sampah Level Rumah Tangga di Kalurahan Baciro, Kota Yogyakarta
Authors: Kusuma Endah Ayu , Hamida Nindya Christy , Widhianta Nanda , Setiawan Qarra Salma , Widinugroho Yudistira 
Published: July 2024

The city of Yogyakarta is one of the contributors to waste at the Piyungan TPST. Waste production in the City of Yogyakarta in 2021 will reach 327 million tons per day with the largest composition of waste being household waste by 55.2%. Baciro Urban Village is one of the sources of waste with a volume of waste generated of 28.57 m3 per day. This study aims to determine the implementation of household waste management in Baciro Village before and after the Zero Inorganic Waste Movement Program policy, factors related to success and obstacles, and the relevance of waste management implementation to health conditions in Baciro Village. The research approach used is a mix method in the form of a combination of qualitative and quantitative approaches. Quantitative analysis was carried out simply by interpreting the data from the questionnaire results in the form of percentage graphs. The results of data processing and analysis show that Baciro Sub-district has implemented the Circular Letter from Pj. Mayor of Yogyakarta Number 660/6123/SE/2022 regarding the Zero Inorganic Waste Movement and succeeded in reducing the final amount of waste disposed of at TPST Piyungan. The existence of the Zero Inorganic Waste Movement Program has brought better development in household waste management in Baciro Kalurahan. The existence of this program has increased community empowerment and the implementation of the waste bank has been able to be monitored to the JSS (Jogja Smart Service) system. However, there are still challenges in terms of regeneration and the contribution of the younger generation. The inhibiting factors of the waste management process are the limited land and waste sorting bins, while the supporting factors for success are if there is sufficient land available and there is an increase in socialization or counseling regarding waste management. The problem of limited land in the implementation of waste banks poses a risk of decreasing public health.
Pengaruh Kualitas Cahaya Dan Frekuensi Aplikasi Paclobutrazol Terhadap Pertumbuhan Dan Hasil Tanaman Krisan (Chrysanthemum morifolium)
Authors: Ratno Dwi , Dyah Ully Parwati Wiwin , Theresia Maria Astuti Yohana , Ryan Ryan Firman Syah 
Published: July 2024

Abstract - This study was to determine the effect of light quality and frequency of paclobutrazol application on the growth and yield of chrysanthemum plants. The research was conducted from February to April 2023 using factorial design and arranged in Split Plot Design consisting of Main Plot and Sub Plot. Main Plot was the effect of light quality using three different light colors, namely white light, red light, and blue light. Sub Plot was the frequency of paclobutrazol application which consists of 4 kinds, namely 0 times, 1 time, 2 times, and 3 times. Data analysis used in this study was analysis of variance with 5% confidence level. The results showed an interaction between the quality of light and the frequency of paclobutrazol application on the parameters of stem diameter and leaf area. Light quality had a good effect on the parameters of plant height, flower color, flower quality, number of flowers, number of leaves and flower diameter. The frequency of paclobutrazol application had a good effect on the parameters of plant height, flower color, flower quality, number of flowers, number of leaves and flower diameter. The best plant height was 106.39 cm, the highest number of leaves was 15.5, the best leaf area was 89.42 cm2, the best stem diameter was 0.41 inches, the best flower color with a score of 2.5, the best flower quality with a score of 2.8, the largest flower diameter was 7.93 cm, and the largest number of flowers was 5 flowers.

Keywords: Light quality, Paclobutrazol, Chrysanthemum, Lamps