bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lipid stability during the frozen storage of fillets from silver catfish exposed in vivo to the essential oil of Lippia alba (Mill.) NE Brown
Authors: Veeck Ana Paula L , Klein Bruna , Ferreira Lauren F , Becker Alexssandro G , Heldwein Clarissa G , Heinzmann Berta M , Baldisserotto Bernardo , Emanuelli Tatiana 
Published: August 2012

AbstractBACKGROUND: Lippia alba is effective in sedating and reducing stress to fish during transportation. Because some in vitro studies have demonstrated the antioxidant activity of L. alba, we hypothesized that its use in vivo could result in antioxidant effects post mortem. Therefore, in this study we evaluated whether the essential oil of L. alba (EO) used as sedative for fish transport would increase the lipid stability of fillets from silver catfish during frozen storage.RESULTS: The exposure to the EO in vivo did not affect conjugated diene values. However, EO (30 and 40 µL L−1) delayed the peak formation of peroxides (from the third to the sixth month of storage) and thiobarbituric reactive substances (from the ninth to the twelfth month of storage) when compared to control fillets. After exposure to 40 µL L−1 EO the free fatty acid content was higher than for control at the start of fillet storage, with no differences among groups thereafter.CONCLUSION: The essential oil of L. alba used as sedative in the water to transport silver catfish can delay lipid oxidation of fillets during frozen storage. Thus L. alba may be a promising source of natural active compounds for use in aquaculture and the food industry. © 2012 Society of Chemical Industry
Quinocetone triggers oxidative stress and induces cytotoxicity and genotoxicity in human peripheral lymphocytes of both genders
Authors: Yang Wei , Fu Juan , Xiao Xiao , Yan Hong , Bao Wei , Wang Di , Hao Liping , Nussler Andreas K , Yao Ping , Liu Liegang 
Published: August 2012

AbstractBackgroundQuinocetone has been widely used as an animal growth promoter in China. However, available data showed that QCT has potential genotoxicity. This study was conducted to investigate the cytotoxicity and genotoxicity of QCT in human lymphocytes.ResultsCCK‐8 assay demonstrated the severe inhibitory effects by QCT in a dose‐ and time‐dependent manner. DNA damage analysis using alkalic Comet assay revealed a pronounced increase of DNA fragmentation in cells. In contrast, DNA damage was significantly decreased after incubation with S9 mix. This finding demonstrated that the intermediate metabolites of this drug exerted lower genotoxicity than its parent drugs. We further described chromosomal damage induced by this drug employing cytokinesis‐block micronucleus assay. The micronucleus frequency was significantly increased in quinocetone groups as compared to controls. Similar to the observation in Comet assay, incorporation of S9 mix in the cytokinesis‐block micronucleus assay could markedly alleviate the chromosomal damage. Moreover, QCT could invoke increase of reactive oxygen species generation in cells. Intriguingly, the toxicity of QCT was more prominent in samples from males than those from females under the same conditions.ConclusionQCT could induce potential cytotoxicity and genotoxicity in human lymphocytes.
Effects of Brazilian green propolis on double‐stranded RNA‐mediated induction of interferon‐inducible gene and inhibition of recruitment of polymorphonuclear cells
Authors: Hayakari Ryo , Matsumiya Tomoh , Xing Fei , Tayone Janeth C , Dempoya Junichi , Tatsuta Tetsuya , Aizawa‐Yashiro Tomomi , Imaizumi Tadaatsu , Yoshida Hidemi , Satoh Kei 
Published: August 2012

AbstractBackgroundPropolis is a bee product with various biological properties, including an antiviral activity when taken orally. However, its mechanisms at the cellular and molecular level are not well understood.ResultsWe investigated the effect of propolis on antiviral signaling in A549 cells transfected with double‐stranded RNA (dsRNA), a model for viral infection. Pretreatment of the cells with propolis inhibited poly I:C (synthetic dsRNA)‐induced interferon (IFN)‐β expression. Propolis had no effect on the dsRNA‐induced expression of RIG‐I‐like receptors (RLRs), which are known as intracellular viral RNA sensors. As to the effect on antiviral executor genes, propolis enhanced myxovirus resistance 1 (MX1) expression, whereas interferon‐inducible gene 6–16 (G1P3) and 2'‐5'‐oligoadenylate synthetase (OAS) were unaffected. All of these genes belong to the IFN‐inducible genes, suggesting that the effect of propolis on antiviral signaling is not necessarily mediated by the autocrine regulation by IFN‐β. Propolis pretreatment inhibited dsRNA‐induced interleukin‐8 (IL8) and CCL5 expression, and consequently lowered polymorphonuclear leukocyte (PMN) chemotactic activity in the cell‐conditioned medium.ConclusionTaken together, these results suggest that propolis may suppress excess inflammatory responses without affecting the innate immunity during viral infection.
Angiotensin I‐converting enzyme inhibitory peptides derived from bovine casein and identified by MALDI‐TOF‐MS/MS
Authors: Wu Zhen , Pan Daodong , Zhen Xiaoqun , Cao Jingxun 
Published: August 2012

AbstractBackgroundHypertension is a major and common threat to the health of individuals around the world. Although agents such as captopril have been shown to regulate high blood pressure effectively, they bring unfavourable side effects such as dry cough and angioedema. If angiotensin I‐converting enzyme (ACE) inhibitors derived from natural substances such as milk proteins can be shown to be safe and efficient at managing hypertension, such inhibitors will be a valuable auxiliary to agents such as captopril.ResultsLow‐molecular‐weight casein‐derived peptides hydrolysed by cell envelope proteinase of Lactobacillus casei subsp. casei (ATCC 15008) showed quite high ACE‐inhibitory activity. The peptide fraction from α‐casein with molecular weight between 5 and 10 kDa showed the highest ACE‐inhibitory activity of 82.35%, with a 50% inhibition concentration (IC50) of 2.36 mg mL−1. Peptides from β‐casein exhibited lower ACE‐inhibitory activity (56.67%, IC50 4.00 mg mL−1). Three distinct peptide sequences derived from α‐casein (αs1‐cn f95–105, f106–115 and f148–166) were identified using two‐dimensional gel electrophoresis coupled with matrix‐assisted laser desorption/ionisation time‐of‐flight tandem mass spectrometry.ConclusionThis work investigated the ACE‐inhibitory properties of casein‐derived peptides. Three distinct peptide sequences derived from α‐casein were identified. Characterisation of such peptides furthers the investigation of casein‐derived ACE‐inhibitory peptides from fermented dairy products.
Effects of dietary sweet potato leaf meal on the growth, non‐specific immune responses, total phenols and antioxidant capacity in channel catfish (Ictalurus punctatus)
Authors: Lochmann Rebecca T. , Islam Shahidul , Phillips Harold , Adam Zelalem , Everette Jace 
Published: August 2012

AbstractBackgroundTraditional energy sources in catfish diets have become costly, and economical alternatives are needed. Sweet potato leaves are underutilised agricultural by‐products that provide energy and substantial amounts of phenols, which affect animal and human health. There is little information on the effects of these compounds on catfish, or the capacity of catfish to accumulate dietary phenols. Catfish enriched with phenols have marketing potential as functional foods. This study investigated the effects of diets with sweet potato leaf meal (SPLM) on growth performance, health and total phenolic compounds in catfish.ResultsSPLM was substituted for wheat middlings in three diets fed to groups of juvenile catfish for 10 weeks. Weight gain, feed conversion, survival, alternative complement activity and lysozyme activity were similar among diets. Haematocrit was lower in fish fed diets with SPLM, but within the normal range. Total phenols and antioxidant capacity in the whole body were similar among treatments.ConclusionSPLM was an effective energy source for catfish up to the maximum level tested (230 g kg−1 diet). SPLM did not enhance total phenols in catfish, but there were no apparent antinutritional effects of the meal on catfish growth, health or survival.
Cochlear nerve deficiency in children with CHARGE syndrome
Authors: Holcomb Meredith A. , Rumboldt Zoran , White David R. 
Published: August 2012

AbstractObjectives/Hypothesis:To describe cochlear nerve deficiency and abnormalities of the internal auditory canal (IAC) associated with CHARGE (coloboma, heart defects, atresia choanae, retardation of growth and/or development, genital hypoplasia, and ear anomalies and/or deafness) syndrome.Study Design:Case series.Methods:In a tertiary children's hospital, children with CHARGE syndrome were evaluated between 2006 and 2009. Morphology of the inner ear, IAC, and cochlear nerves was evaluated along with auditory‐evoked brainstem response results.Results:Of the 17 patients with CHARGE diagnosis, 14 ears demonstrated profound sensorineural hearing loss (SNHL). The remaining ears had conductive hearing loss, mixed hearing loss, or mild to moderate SNHL. Computed tomography (CT) evaluation was refused in one case and delayed in one case due to parental decision. Of 30 ears evaluated with CT, 28 (93%) had substantial abnormalities of the inner ear including hypoplasia or aplasia of the semicircular canals and abnormalities of the cochlea and vestibule. CT evaluation revealed cochlear aperture narrowing or occlusion in 16 ears, one of which had normal hearing. The eight most recent patients with profound SNHL (six bilateral SNHL, two unilateral) underwent magnetic resonance imaging (MRI) of the IAC, and 13 of 14 ears with SNHL were noted to have absent or deficient cochlear nerves. CT scans in two ears with profound SNHL revealed normal cochlear apertures.Conclusions:The dysmorphic inner ear is well described in CHARGE patients; however, cochlear nerve deficiency has not been considered a common etiology for SNHL in these patients. Because of the implications of cochlear nerve deficiency in therapeutic decision making (i.e., cochlear implantation), MRI evaluation of the eighth nerve should be considered in CHARGE patients with profound SNHL. Laryngoscope, 2013
In vivo imaging of photoreceptor disruption associated with age‐related macular degeneration: A pilot study
Authors: Boretsky Adam , Khan Faraz , Burnett Garrett , Hammer Daniel X. , Ferguson R. Daniel , van Kuijk Frederik , Motamedi Massoud 
Published: August 2012

AbstractBackground and ObjectiveAge‐related macular degeneration is one of the leading causes of vision loss in the developed world. As the disease progresses, the central part of the retina, called the macula, is compromised leading to a disruption of both structure and visual function. In this study, we investigate the disruption of macular photoreceptor cells in vivo as a function of disease stage in patients with the dry form of age‐related macular degeneration AMD.Materials and MethodsAn investigational confocal Adaptive Optics Scanning Laser Ophthalmoscope (AO–SLO) was used to obtain high resolution images of the macular photoreceptor mosaic in patients previously diagnosed with AMD. Four patients were selected as representative cases, comprising each of the four clinical stages of AMD progression.ResultsAO–SLO imaging revealed slight disruption in the photoreceptor mosaic in early stage AMD due to focal drusen formation and identified several small drusen deposits that were not observed with standard clinical imaging techniques. An increase in photoreceptor disruption was visualized within the macula in direct correlation with the stage of AMD progression leading to a decrease in visual acuity. Large coalescent drusen and areas of geographic atrophy in advanced stage dry AMD exhibited a significant decrease in visible photoreceptor density. Significant decrease in photoreceptor counts (∼35–50%) were observed when comparing earlier stages of AMD progression (Categories I and II) to later stages of the disease (Categories III and IV).ConclusionsThis study demonstrates the capabilities of adaptive optics retinal imaging to monitor disruption of individual photoreceptor cells as a function of disease progression yielding valuable diagnostic findings in early stage AMD beyond what can be learned about the health of photoreceptors using conventional retinal imaging techniques. Lasers Surg. Med. 44: 603–610, 2012. © 2012 Wiley Periodicals, Inc.
Construction of Donor–Acceptor Chromophores in Platinum Polyyne Polymer by [2 + 2] Cycloaddition of Organic Acceptors
Authors: Yuan Yuping , Michinobu Tsuyoshi 
Published: August 2012

AbstractA thiophene‐containing platinum polyyne polymer was postfunctionalized by a [2 + 2] cycloaddition followed by a cycloreversion of tetracyanoethylene (TCNE) or 7,7,8,8‐tetracyanoquinodimethane (TCNQ), yielding narrow band gap polymers. The quantitative addition of the acceptor molecules was confirmed by combined analytical techniques. The postfunctionalized platinum polyyne polymers displayed charge‐transfer (CT) bands in the visible‐near IR region and potent redox activities. The TCNQ adduct has a stronger electron‐accepting feature than the TCNE adduct, as revealed by the more bathochromically shifted CT band and the lower first reduction potential. The systematic investigation revealed a clear correlation between the optical and electrochemical band gaps.
High‐Shear‐Strength Waterborne Polyurethane/Acrylic Soft Adhesives
Authors: Degrandi‐Contraires Elise , Lopez Aitziber , Reyes Yuri , Asua José M. , Creton Costantino 
Published: August 2012

AbstractUrethane/acrylic hybrid latex particles are prepared by miniemulsion polymerization for an application as soft adhesives. The polymerization of the acrylic monomers and grafting of an isocyanate functionalized PU on a hydroxyl functionalized monomer (HEMA) take place simultaneously, resulting in a complex PU/acrylic network while avoiding any macroscopic phase separation. Its structure can be tuned by changing the extent of grafting and a specific model is applied to analyze the final polymer microstructure. The resulting materials have a low level of adhesion but display an exceptionally high resistance to shear. Two parameters are varied: the fraction of HEMA in the monomer composition and the diol concentration. magnified image
Celebrities in Advertising: Looking for Congruence or Likability?
Authors: Fleck Nathalie , Korchia Michael , Le Roy Isabelle 
Published: August 2012

ABSTRACTThe choice of a celebrity endorser for a brand is an important topic in advertising and marketing, as considerable time and effort resources are dedicated to finding the right celebrity to represent a given organization. Celebrities used as endorsers in advertisements are often very popular ones. However, from a cognitive point of view (and a more academic one), congruence between brand and celebrity seems to be very important too. Based on affective and cognitive theories to explain endorsement effectiveness, congruence between brand and celebrity is shown to be as effective as celebrity likability. Moreover, congruence between brand and celebrity as well as celebrity likability have an impact on the predisposition toward the ad, which in turn influences brand beliefs and purchase intention.
Celebrity Endorsements and Beyond: New Avenues for Celebrity Branding
Authors: Keel Astrid , Nataraajan Rajan 
Published: August 2012

ABSTRACTThe authors assess the extant research in the area of celebrity endorsement and point out the need for continuing research in celebrity marketing. Suggestions for future research are made in a wide breadth of areas, spanning from celebrity endorsements to novel areas of celebrity branding. The authors propose three celebrity‐branding strategies and discuss the factors that may influence their effectiveness. Other areas for future research in celebrity marketing that are discussed include ethical marketing to vulnerable consumers and social marketing. The authors conclude with suggestions regarding theories and methods that can be used for future research in celebrity marketing.
A subset of papillary thyroid carcinomas contain KRAS mutant subpopulations at levels above normal thyroid
Authors: Myers Meagan B. , McKim Karen L. , Parsons Barbara L. 
Published: August 2012

AbstractThe molecular pathogenesis of papillary thyroid carcinoma (PTC) is largely attributed to chromosomal rearrangements and point mutations in genes within the MAPK pathway (i.e., BRAF and RAS). Despite KRAS being the 6th most frequently mutated gene for all cancers, the reported frequency in thyroid cancer is only 2%. This may be due, in part, to the use of insensitive mutation detection methods such as DNA sequencing. Therefore, using the sensitive and quantitative ACB‐PCR approach, we quantified KRAS codon 12 GGT → GAT and GGT → GTT mutant fraction (MF) in 20 normal thyroid tissues, 17 primary PTC, 2 metastatic PTC, and 1 anaplastic thyroid carcinoma. We observed measurable levels of KRAS codon 12 GAT or GTT mutation in all normal thyroid tissues. For PTCs, 29.4% and 35.3% had KRAS codon 12 GAT and GTT MF above the 95% upper confidence interval for the corresponding MFs in normal thyroid. The highest observed KRAS codon 12 GTT MFs were associated with tumors with follicular characteristics and relatively high levels of tumor necrosis. The results indicate KRAS mutant subpopulations are present in a large number of thyroid tumors, a fact previously unrecognized. The presence of KRAS mutation may indicate a tumor with an aggressive phenotype, thus directing the course of clinical treatment. © 2012 Wiley Periodicals, Inc.
Immediate force loss after eccentric contractions is increased with l‐name administration, a nitric oxide synthase inhibitor
Authors: Corona Benjamin T. , Ingalls Christopher P. 
Published: August 2012

AbstractIntroductionNitric oxide (NO) signaling regulates many biological processes in skeletal muscle, wherein aberrant signaling contributes to myopathic conditions (e.g., Duchenne muscular dystrophy). NO has been shown to play a role in muscle regeneration after injury. However, less is known about its role during injury. In this study we aimed to determine whether NO synthase (NOS) inhibition exacerbates functional deficits immediately after the performance of eccentric contractions.MethodsWild‐type mouse extensor digitorum longus (EDL) muscles underwent in vitro functional testing in the presence or absence of a non‐specific NOS inhibitor (L‐NAME, 10 mM) before and after performance of 10 eccentric contractions.ResultsAfter eccentric contractions, Po was reduced by ∽25% for muscle in regular physiological solution but by ∽50% with the addition of L‐NAME (P = 0.009).ConclusionsNon‐specific blockade of NOS exacerbates functional deficits immediately after eccentric contractions, suggesting that NO signaling protects skeletal muscle from excessive injury in healthy muscle. Muscle Nerve, 2013
Falls and spinal muscular atrophy: Exploring cause and prevention
Authors: Montes Jacqueline , Mcisaac Tara L. , Dunaway Sally , Kamil‐Rosenberg Shirit , Sproule Douglas , Garber Carol Ewing , De vivo Darryl C. , Rao Ashwini K. 
Published: August 2012

AbstractIntroduction: Falls can cause injury and may compromise function in spinal muscular atrophy (SMA) patients. Weakness and gait variability are associated with falls in other neurological disorders, and fatigue is well documented in SMA. The relationship of weakness, fatigue, and gait variability to falls has never been investigated. Methods: Seven ambulatory patients with SMA completed a falls history questionnaire, 6MWT, gait analysis, and strength testing. Pearson correlation coefficients were used to examine associations between these variables. Results: All 7 subjects reported falls in the previous year. Stride‐length variability was significantly associated with falls, unlike strength, fatigue, or other gait variables. Conclusions: Stride‐length variability was the key variable associated with falls. Preventive strategies to avoid falls should be incorporated into patient management plans. Gait analysis provides actionable information not revealed by standard assessments and should be included in clinical trials designed to address the prevention of falls in the SMA population. Muscle Nerve, 2013
A kinematic hardening soil model considering the principal stress rotation
Authors: Yang Yunming , Yu Hai‐Sui 
Published: August 2012

SUMMARYIn this article, a typical and representative kinematic hardening soil model is first studied to investigate its capabilities to reproduce soil responses under principal stress rotations (PSR). It is found that the model is capable of reproducing the non‐coaxiality very well. It can qualitatively capture the trends of responses of different stress and strain components, without the capability to quantitatively reproduce these responses. Its prediction of volumetric responses is the poorest and can give wrong results in many cases. The underlying reasons for these capabilities and defects are analyzed in detail. The model is subsequently modified to better take into account the PSR influences. An additional new flow rule and plastic modulus for the PSR are developed. One important feature of the model is that it is developed in the general stress space with six stress variables. Therefore, it can take into account multiple PSRs at different directions. Another feature is that it retains the linear stress rate–strain rate relationship, which facilitates its numerical implementations. In addition, the universal characteristic of the theory makes it equally applicable to other kinematic hardening models. Copyright © 2012 John Wiley & Sons, Ltd.
Integrated job scheduling and network routing
Authors: Gamst Mette , Pisinger David 
Published: August 2012

AbstractWe consider an integrated job scheduling and network routing problem which appears in Grid Computing and production planning. The problem is to schedule a number of jobs at a finite set of machines, such that the overall profit of the executed jobs is maximized. Each job demands a number of resources which must be sent to the executing machine through a network with limited capacity. A job cannot start before all of its resources have arrived at the machine.The scheduling problem is formulated as a Mixed Integer Program (MIP) and proved to be
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‐hard. An exact solution approach using Dantzig‐Wolfe decomposition is presented. The pricing problem is the linear multicommodity flow problem defined on a time‐space network. Branching strategies are presented for the branch‐and‐price algorithm and three heuristics and an exact solution method are implemented for finding a feasible start solution. Finally, interior point stabilization is used to decrease the number of columns generated in the branch‐and‐price algorithm.The algorithm is experimentally evaluated on job scheduling instances for a Grid network. The Dantzig‐Wolfe algorithm with stabilization is clearly superior, being able to solve large instances with 1,000 jobs and 1,000 machines covering 24 hours of scheduling activity on a Grid network. The algorithm is also compared to simulations of a real‐life Grid, and results show that the solution quality significantly increases when solving the problem to optimality. The promising results indicate that the algorithm can be used as an actual scheduling algorithm in the Grid or as a tool for analyzing Grid performance when adding extra machines or jobs. © 2012 Wiley Periodicals, Inc. NETWORKS, 2013