bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sensitivity of Real-Data Simulations of the 3 May 1999 Oklahoma City Tornadic Supercell and Associated Tornadoes to Multimoment Microphysics. Part I: Storm- and Tornado-Scale Numerical Forecasts
Authors: Dawson Daniel T. , Xue Ming , Milbrandt Jason A. , Shapiro Alan 
Published: March 2015

Abstract
Numerical predictions of the 3 May 1999 Oklahoma City, Oklahoma, tornadic supercell are performed within a real-data framework utilizing telescoping nested grids of 3-km, 1-km, and 250-m horizontal spacing. Radar reflectivity and radial velocity from the Oklahoma City WSR-88D are assimilated using a cloud analysis procedure coupled with a cycled 3DVAR system to analyze storms on the 1-km grid for subsequent forecast periods. Single-, double-, and triple-moment configurations of a multimoment bulk microphysics scheme are used in several experiments on the 1-km and 250-m grids to assess the impact of varying the complexity of the microphysics scheme on the storm structure, behavior, and tornadic activity (on the 250-m grid). This appears to be the first study of its type to investigate single- versus multimoment microphysics within a real-data context.
It is found that the triple-moment scheme overall performs the best, producing the smallest track errors for the mesocyclone on the 1-km grid, and stronger and longer-lived tornado-like vortices (TLVs) on the 250-m grid, closest to the observed tornado. In contrast, the single-moment scheme with the default Marshall–Palmer rain intercept parameter performs poorly, producing a cold pool that is too strong, and only weak and short-lived TLVs. The results in the context of differences in latent cooling from evaporation and melting between the schemes, as well as implications for numerical prediction of tornadoes, are discussed. More generally, the feedbacks to storm thermodynamics and dynamics from increasing the prognostic detail of the hydrometeor size distributions are found to be important for improving the simulation and prediction of tornadic thunderstorms.
Climatological Characteristics and Observed Trends of Tropical Cyclone–Induced Rainfall and Their Influences on Long-Term Rainfall Variations in Hong Kong
Authors: Li Richard C. Y. , Zhou Wen , Lee Tsz Cheung 
Published: March 2015

Abstract
This study examines the climatological features of tropical cyclone (TC) rainfall in Hong Kong in association with different TC-related parameters, and investigates the changes in TC rainfall, non-TC rainfall, and total rainfall during the past few decades in Hong Kong. On average, rainfall induced by TCs can account for about 25% of the total precipitation during summer and fall, and the contribution can be even greater in extreme cases. Composite analysis suggests that extreme TC rainfall is often related to TCs in closer proximity to Hong Kong, with higher intensity, and is associated with stronger convection and moisture convergence in the vicinity of Hong Kong.
Evaluations of the observed trends of different rainfall indices suggest that the rainfall variability in Hong Kong is considerably affected by the TC rainfall, which has a decreasing trend in frequency and intensity in recent decades. Taking out the TC rainfall from the total rainfall reveals that there is an increasing trend in daily rainfall frequency and intensity for non-TC rainfall in Hong Kong. Moreover, time-dependent generalized extreme value analysis of non-TC rainfall also reveals an increase in the return values of the maximum daily rainfall in Hong Kong. Results of this study suggest that, in order to obtain a more comprehensive picture of the long-term rainfall variations in Hong Kong, the contributions of TC rainfall should definitely be taken into account in the analysis.
Arbitrary-Order Conservative and Consistent Remapping and a Theory of Linear Maps: Part I
Authors: Ullrich Paul A. , Taylor Mark A. 
Published: March 2015

Abstract
The design of accurate, conservative, consistent, and monotone operators for remapping scalar fields between computational grids on the sphere has been a persistent issue for global modeling groups. This problem is especially pronounced when mapping between distinct discretizations (such as finite volumes or finite elements). To this end, this paper provides a novel unified mathematical framework for the development of linear remapping operators. This framework is then applied in the development of high-order conservative, consistent, and monotone linear remapping operators from a finite-element discretization to a finite-volume discretization. The resulting scheme is evaluated in the context of both idealized and operational simulations and shown to perform well for a variety of problems.
Acute Kidney Injury in the Critically Ill Patient
Authors: Pakula Andrea M. , Skinner Ruby A. 
Published: March 2015

Purpose: A comprehensive review of the literature to provide a focused and thorough update on the issue of acute kidney injury (AKI) in the surgical patient. Methods: A PubMed and Medline search was performed and keywords included AKI, renal failure, critically ill, and renal replacement therapy (RRT). Principal Findings: A common clinical problem encountered in critically ill patients is AKI. The recent consensus definitions for the diagnosis and classification of AKI (ie, Risk, Injury, Failure, Loss of kidney function, and End-stage kidney disease/Acute Kidney Injury Network) have enabled us to standardize the severity of AKI and facilitate strategies for prevention. These strategies as well as treatment modalities of AKI are discussed. We provide a concise overview of the issue of renal failure. We describe strategies for prevention including types of fluids used for resuscitation, timing of initiation of RRT, and different treatment modalities currently available for clinical practice. Conclusions: Acute kidney injury is a common problem in the critically ill patient and is associated with worse clinical outcomes. A standardized definition and staging system has led to improved diagnosis and understanding of the pathophysiology of AKI. There are many trials leading to improved prevention and management of the disease.
A Pilot Curriculum to Integrate Standardized Patient Simulation into Clinical Pastoral Education
Authors: Ahmed Rami A. , Damore Deborah R. , Viti Joseph F. , Hughes Patrick G. , Miesle Rebecca , Ataya Ramsey , Atkinson S. Scott , Gable Brad 
Published: March 2015

Introduction: We describe a novel means of experiential learning for clinical pastoral care residents using standardized patient (SP) simulations. Methods: A prospective cohort study involving 7 clinical pastoral care residents was performed. All residents underwent 2 verbatim SP sessions and 2 simulation sessions. After all sessions, residents completed a self-evaluation. Faculty completed an evaluation and then provided a debriefing session to all residents. Results: Performance ratings were globally higher on simulated scenarios when compared to the verbatim sessions. Conclusions: More research in the field of pastoral care is needed to validate the learned professional skills that enhance a comprehensive training program through the use of medical simulation, verbatim reports, and clinical pastoral education (CPE) competencies. Medical simulation provides a promising teaching methodology for the training of CPE residents.
Psoriatic Nail Changes Are Associated With Clinical Outcomes in Psoriatic Arthritis
Authors: Sandre Matthew K. , Rohekar Sherry , Guenther Lyn 
Published: March 2015

Background: Nail changes are common in psoriatic arthritis (PsA), with varying relationships existing between skin, nail, and joint disease. Objective: To further characterize relationships between nail changes, psoriasis, and joint involvement in PsA patients. Methods: One hundred eighty-eight PsA patients had skin, fingernail, and rheumatological assessments completed. Hand and fingernail photographs were taken and reviewed by a dermatologist. Results: Higher swollen joint counts were associated with distal interphalangeal (DIP)/periungual psoriasis ( P = .001), more splinter hemorrhages ( P = .006), and any nail bed change ( P = .03). Higher tender joint counts were associated with rough onychorrhexis ( P < .001), DIP/periungual psoriasis ( P = .03), red lunula ( P = .001), nail crumbling ( P = .046), any nail matrix ( P = .03), and nail bed change ( P = .03). Joint involvement was associated with same-digit nail changes; strongest association was swollen or tender DIP with subungual hyperkeratosis, odds ratio = 26.6 (95% CI, 5.1-139.1). Conclusion: The DIP/periungual psoriasis and specific nail changes were associated with higher joint counts and certain nail changes with same-digit joint involvement.
Trends in Ambulatory Health Care Usage for Adult Acne
Authors: Davis Scott A. , Huang Karen E. , Feldman Steven R. , Fleischer Alan B. , Huang William W. 
Published: March 2015

Background: The prevalence of acne is increasing among young children but not well characterized in older patients. Objective: To determine whether ambulatory health care for adult acne is increasing and whether the average age of adult acne patients seeking care has been increasing over time. Methods: We performed a retrospective analysis of data from the 1993-2010 National Ambulatory Medical Care Survey for acne visits in ages 25 and older. Results: Average age of adult acne patients increased by 0.13 years per year over the study period. Visits per 100 000 population declined in the 25 to 34 age group ( P = .05) and did not change significantly in the 35 to 44 and 45 and older age groups. Conclusions: The hypothesis that adult acne visits are increasing was not supported, but the average age of adult acne patients seeking care has been rising.
The Rise and Fall of Oral Ketoconazole
Authors: Gupta Aditya K. , Lyons Danika C.A. 
Published: March 2015

Background: Ketoconazole was the first broad-spectrum oral antifungal agent available to treat systemic and superficial mycoses. Evidence of hepatotoxicity associated with its use emerged within the first few years of its approval. Growing evidence of serious side effects including endocrine dysregulation, several drug interactions, and death led to the review of oral ketoconazole in 2011. Objective: This article chronicles the use of oral ketoconazole from its introduction to its near replacement in medicine. Conclusion: Due to its hepatotoxic side effects, oral ketoconazole was withdrawn from the European and Australian markets in 2013. The United States imposed strict relabeling requirements and restrictions for prescription, with Canada issuing a risk communication echoing these concerns. Today, oral ketoconazole is only indicated for endemic mycoses, where alternatives are not available or feasible. Meanwhile, topical ketoconazole is effective, safe, and widely prescribed for superficial mycoses, particularly as the first-line treatment for tinea versicolor.
The Bruton tyrosine kinase inhibitor ibrutinib with chemoimmunotherapy in patients with chronic lymphocytic leukemia
Authors: Brown Jennifer R. , Barrientos Jacqueline C. , Barr Paul M. , Flinn Ian W. , Burger Jan A. , Tran Anh , Clow Fong , James Danelle F. , Graef Thorsten , Friedberg Jonathan W. , Rai Kanti , O’Brien Susan 
Published: March 2015

Key Points
Ibrutinib was well tolerated when administered with BR CIT in previously treated chronic lymphocytic leukemia/small lymphocytic lymphoma. Ibrutinib added to CIT was associated with a high degree of clinical activity that compares favorably to historical reports of CIT alone.
Intriguing photo-control of exchange bias in BiFeO3/La2/3Sr1/3MnO3 thin films on SrTiO3 substrates
Authors: Sung Kil Dong , Lee Tae Kwon , Jung Jong Hoon 
Published: March 2015

Abstract
To date, electric fields have been widely used to control the magnetic properties of BiFeO3-based antiferromagnet/ferromagnet heterostructures through application of an exchange bias. To extend the applicability of exchange bias, however, an alternative mechanism to electric fields is required. Here, we report the photo-control of exchange bias in BiFeO3/La2/3Sr1/3MnO3 thin films on an SrTiO3 substrate. Through an ex situ pulsed laser deposition technique, we successfully synthesized epitaxial BiFeO3/La2/3Sr1/3MnO3 thin films on SrTiO3 substrates. By measuring magnetoresistance under light illumination, we investigated the effect of light illumination on resistance, exchange bias, and coercive field in BiFeO3/La2/3Sr1/3MnO3 thin films. After illumination of red and blue lights, the exchange bias was sharply reduced compared to that measured in the dark. With increasing light intensity, the exchange bias under red and blue lights initially decreased to zero and then appeared again. It is possible to reasonably explain these behaviors by considering photo-injection from SrTiO3 and the photo-conductivity of La2/3Sr1/3MnO3. This study may provide a fundamental understanding of the mechanism underlying photo-controlled exchange bias, which is significant for the development of new functional spintronic devices.
Lectin RCA-I specifically binds to metastasis-associated cell surface glycans in triple-negative breast cancer
Authors: Zhou Shu-Min , Cheng Li , Guo Shu-Juan , Wang Yang , Czajkowsky Daniel M , Gao Huafang , Hu Xiao-Fang , Tao Sheng-Ce 
Published: March 2015

Abstract
Introduction
Triple-negative breast cancer (TNBC) patients often face a high risk of early relapse characterized by extensive metastasis. Previous works have shown that aberrant cell surface glycosylation is associated with cancer metastasis, suggesting that altered glycosylations might serve as diagnostic signatures of metastatic potential. To address this question, we took TNBC as an example and analyzed six TNBC cell lines, derived from a common progenitor, that differ in metastatic potential.

Methods
We used a microarray with 91 lectins to screen for altered lectin bindings to the six TNBC cell lines. Candidate lectins were then verified by lectin-based flow cytometry and immunofluorescent staining assays using both TNBC/non-TNBC cancer cells. Patient-derived tissue microarrays were then employed to analyze whether the staining of Ricinus communis agglutinin I (RCA-I), correlated with TNBC severity. We also carried out real-time cell motility assays in the presence of RCA-I. Finally, liquid chromatography-mass spectrometry/tandem spectrometry (LC-MS/MS) was employed to identify the membrane glycoproteins recognized by RCA-I.

Results
Using the lectin microarray, we found that the bindings of RCA-I to TNBC cells are proportional to their metastatic capacity. Tissue microarray experiments showed that the intensity of RCA-I staining is positively correlated with the TNM grades. The real-time cell motility assays clearly demonstrated RCA-I inhibition of adhesion, migration, and invasion of TNBC cells of high metastatic capacity. Additionally, a membrane glycoprotein, POTE ankyrin domain family member F (POTEF), with different galactosylation extents in high/low metastatic TNBC cells was identified by LC-MS/MS as a binder of RCA-I.

Conclusions
We discovered RCA-I, which bound to TNBC cells to a degree that is proportional to their metastatic capacities, and found that this binding inhibits the cell invasion, migration, and adhesion, and identified a membrane protein, POTEF, which may play a key role in mediating these effects. These results thus indicate that RCA-I-specific cell surface glycoproteins may play a critical role in TNBC metastasis and that the extent of RCA-I cell binding could be used in diagnosis to predict the likelihood of developing metastases in TNBC patients.
Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium
Authors: Li Zhenzhen , Han Shichao , Wang Xingqin , Han Fu , Zhu Xiongxiang , Zheng Zhao , Wang Hongtao , Zhou Qin , Wang Yunchuan , Su Linlin , Shi Jihong , Tang Chaowu , Hu Dahai 
Published: March 2015

Abstract
Introduction
Bone marrow mesenchymal stem cells (BMSCs), which have the ability to self-renew and to differentiate into multiple cell types, have recently become a novel strategy for cell-based therapies. The differentiation of BMSCs into keratinocytes may be beneficial for patients with burns, disease, or trauma. However, the currently available cells are exposed to animal materials during their cultivation and induction. These xeno-contaminations severely limit their clinical outcomes. Previous studies have shown that the Rho kinase (ROCK) inhibitor Y-27632 can promote induction efficiency and regulate the self-renewal and differentiation of stem cells. In the present study, we attempted to establish a xeno-free system for the differentiation of BMSCs into keratinocytes and to investigate whether Y-27632 can facilitate this differentiation.

Methods
BMSCs isolated from patients were cultured by using a xeno-free system and characterised by using flow cytometric analysis and adipogenic and osteogenic differentiation assays. Human primary keratinocytes were also isolated from patients. Then, the morphology, population doubling time, and β-galactosidase staining level of these cells were evaluated in the presence or absence of Y-27632 to determine the effects of Y-27632 on the state of the keratinocytes. Keratinocyte-like cells (KLCs) were detected at different time points by immunocytofluorescence analysis. Moreover, the efficiency of BMSC differentiation under different conditions was measured by quantitative real-time-polymerase chain reaction (RT-PCR) and Western blot analyses.

Results
The ROCK inhibitor Y-27632 promoted the proliferation and lifespan of human primary keratinocytes. In addition, we showed that keratinocyte-specific markers could be detected in BMSCs cultured in a xeno-free system using keratinocyte-conditioned medium (KCM) independent of the presence of Y-27632. However, the efficiency of the differentiation of BMSCs into KLCs was significantly higher in the presence of Y-27632 using immunofluorescence, quantitative RT-PCR, and Western blot analyses.

Conclusions
This study demonstrated that Y-27632 could promote the proliferation and survival of human primary keratinocytes in a xeno-free culture system. In addition, we found that BMSCs have the ability to differentiate into KLCs in KCM and that Y-27632 can facilitate this differentiation. Our results suggest that BMSCs are capable of differentiating into KLCs in vitro and that the ROCK pathway may play a critical role in this process.
Mesenchymal stem cell-derived microparticles ameliorate peritubular capillary rarefaction via inhibition of endothelial-mesenchymal transition and decrease tubulointerstitial fibrosis in unilateral ureteral obstruction
Authors: Choi Hoon Young , Lee Hyun Gyu , Kim Beom Seok , Ahn Sun Hee , Jung Ara , Lee Mirae , Lee Jung Eun , Kim Hyung Jong , Ha Sung Kyu , Park Hyeong Cheon 
Published: March 2015

Abstract
Introduction
Microparticles (MPs) derived from kidney-derived mesenchymal stem cells (KMSCs) have recently been reported to ameliorate rarefaction of peritubular capillaries (PTC) in ischemic kidneys via delivery of proangiogenic effectors. This study aimed to investigate whether KMSC-derived MPs show anti-fibrotic effects by ameliorating endothelial-to-mesenchymal transition (EndoMT) in human umbilical vein endothelial cells (HUVEC) in vitro and by preserving PTC in kidneys with unilateral ureteral obstruction (UUO) in vivo.

Methods
MPs isolated from the supernatants of KMSC were co-cultured with HUVEC to assess their in vitro biologic effects on endothelial cells. Mice were treated with MPs via the tail vein after UUO injury to assess their anti-fibrotic and PTC sparing effects. Renal tubulointerstitial damage and inflammatory cell infiltration were examined with Masson’s trichrome, F4/80 and α-smooth muscle actin (α-SMA) staining and PTC rarefaction index was determined by CD31 staining.

Results
KMSC-derived MPs significantly ameliorated EndoMT and improved in vitro proliferation of TGF-β1 treated HUVEC. In vivo administration of KMSC-derived MPs significantly inhibited EndoMT of PTC endothelial cells and improved PTC rarefaction in UUO kidneys. Furthermore, administration of KMSC-derived MPs inhibited inflammatory cell infiltration as well as tubulointerstitial fibrosis in UUO mice as demonstrated by decreased F4/80 and α-SMA-positive cells and Masson’s trichrome staining, respectively.

Conclusions
Our results suggest that KMSC-derived MPs ameliorate PTC rarefaction via inhibition of EndoMT and protect against progression of renal damage by inhibiting tubulointerstitial fibrosis.
Jagged-1 is required for the expansion of CD4+ CD25+ FoxP3+ regulatory T cells and tolerogenic dendritic cells by murine mesenchymal stromal cells
Authors: Cahill Emer F , Tobin Laura M , Carty Fiona , Mahon Bernard P , English Karen 
Published: March 2015

Abstract
Introduction
Mesenchymal stromal cells (MSC) have well defined immunomodulatory properties including the suppression of lymphocyte proliferation and inhibition of dendritic cell (DC) maturation involving both cell contact and soluble factors. These properties have made MSC attractive candidates for cellular therapy. However, the mechanism underlying these characteristics remains unclear. This study sought to investigate the mechanisms by which MSC induce a regulatory environment.

Method
Allogeneic bone marrow mesenchymal stromal cells were cultured with T cells or dendritic cells in the presence or absence of gamma secretase inhibitor to block Notch receptor signalling. T cells and dendritic cells were examined by flow cytometry for changes in phenotype marker expression. Stable knock down MSC were generated to examine the influence of Jagged 1 signalling by MSC. Both wildtype and knockdown MSC were subsequently used in vivo in an animal model of allergic airway inflammation.

Results
The Notch ligand Jagged-1 was demonstrated to be involved in MSC expansion of regulatory T cells (Treg). Additionally, MSC-induced a functional semi-mature DC phenotype, which further required Notch signalling for the expansion of Treg. MSC, but not Jagged-1 knock down MSC, reduced pathology in a mouse model of allergic airway inflammation. Protection mediated by MSC was associated with enhanced Treg in the lung and significantly increased production of interleukin (IL)-10 in splenocytes re-stimulated with allergen. Significantly less Treg and IL-10 was observed in mice treated with Jagged-1 knock down MSC.

Conclusions
The current study suggests that MSC-mediated immune modulation involves the education and expansion of regulatory immune cells in a Jagged-1 dependent manner and provides the first report of the importance of Jagged-1 signalling in MSC protection against inflammation in vivo.